bluuebunny/crossref_metadata_2025 huggingface dataset:
Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica
Authors: Abdulrasheed Mansur , Zakaria Nur Nadhirah , Ahmad Roslee Ahmad Fareez , Shukor Mohd Yunus , Zulkharnain Azham , Napis Suhaimi , Convey Peter , Alias Siti Aisyah , Gonzalez-Rocha Gerardo , Ahmad Siti Aqlima
Published: April 2020

Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures.
Report from the COVID-19 Virtual Summit, Disaster Experts Speak Out, March 31, 2020
Authors: Phillips James P. , Ragazzoni Luca , Burel W. Greg , Burkle Frederick M. , Keim Mark
Published: April 2020

This article captures the webinar narrative on March 31, 2020 of four expert panelists addressing three questions on the current coronavirus disease 2019 (COVID-19) pandemic. Each panelist was selected for their unique personal expertise, ranging from front-line emergency physicians from multiple countries, an international media personality, former director of the US Strategic National Stockpile, and one of the foremost international experts in disaster medicine and public policy. The forum was moderated by one of the most widely recognized disaster medical experts in the world. The four panelists were asked three questions regarding the current pandemic as follows:

1.

What do you see as a particular issue of concern during the current pandemic?

2.

What do you see as a particular strength during the current pandemic?

3.

If you could change one thing about the way that the pandemic response is occurring, what would you change?
Challenging pollution and the balance problem from rare earth extraction: how recycling and environmental taxation matter
Authors: Ba Bocar Samba , Combes-Motel Pascale , Schwartz Sonia
Published: April 2020

Rare earth element extraction induces environmental damages and the balance problem. In this article, we show that recycling can challenge both problems in a two-period framework. We also find other results depending on the amount of scrap that can be recycled. If the recycling activity is not limited by available scrap, it does not change extraction in the first period. Environmental taxes on extracted quantities reduce extraction and favor recycling. But if the recycling is limited, the extractor reduces extraction in period one, adopting a foreclosure strategy, and environmental taxes can decrease recycling. In all cases, environmental taxes are never equal to the marginal damage from pollution, in order to take into account the recycling effect.
More to offer than books: stakeholder perceptions of a public library-based meal programme
Authors: De La Cruz Monica M , Phan Kimmy , Bruce Janine S
Published: April 2020

Objective:

To examine the perspectives of librarians and staff aboutLunch at the Library, a library-based summer meal programme for children. The study examines: (i) motivating factors behind implementing the meal programme; (ii) issues of feasibility; and (iii) perceived programme outcomes.

Design:

One-on-one semi-structured interviews with library stakeholders (librarians and staff) from a purposeful sample of California libraries.

Setting:

Twenty-two library jurisdictions across California that implemented theLunch at the Librarysummer meal programme in 2015 in areas of high financial need.

Participants:

Twenty-five library stakeholders representing twenty-two of the thirty-three Californian library jurisdictions that implementedLunch at the Libraryat their sites.

Results:

Library stakeholders recognised the need for a child meal programme during summer. Despite lack of sufficient resources and personnel, they were motivated to implement the programme not only to fill a community need but also to ensure children at their libraries were primed for learning over the summer. Library stakeholders also perceived the public library’s changing role in society as shifting from reference provision to social service provision either directly or by referral.

Conclusions:

The public library is an ideal place to provide social services because of its accessibility to all. Librarians and library staff are motivated to address the social needs of their communities. This study demonstrates the feasibility of implementing new social programmes at public libraries. Funding to support these programmes would increase the library’s capacity to address other community needs.
The importance of school lunches to the overall dietary intake of children in Sweden: a nationally representative study
Authors: Eustachio Colombo Patricia , Patterson Emma , Elinder Liselotte S , Lindroos Anna Karin
Published: April 2020

Objective:

School lunches have potential to foster healthy diets in all children, but data on their importance are relatively scarce. The current study aimed to describe the dietary intake from school lunches by sex and school grade, and to assess how the daily intake, school lunch intake and the daily intake provided by lunch differ by sex and parental education.

Design:

Cross-sectional. All foods and drinks consumed for 1–3 weekdays were self-reported. Energy, absolute and energy-adjusted intakes of nutrients and food groups were calculated per weekday and per school lunch. Mixed-effects linear models assessed sociodemographic differences in dietary intakes. Nutrient and energy density at lunch and during the rest of the day were compared.

Setting:

Seventy-nine Swedish primary schools.

Participants:

Pupils in grades 5 and 8 (N2002), nationally representative.

Results:

Lunch provided around half of daily vegetable intake and two-thirds of daily fish intake. Nutrient density was higher and energy density lower at lunch compared with the rest of the day (P< 0·001). Boys had greater energy-adjusted intakes of red/processed meat and lower intakes of vegetables and dietary fibre compared with girls (P< 0·001), overall and at lunch. Daily energy-adjusted intakes of most nutrients/food groups were lower for pupils of lower-educated parents compared with pupils of parents with higher education, but at lunch, only Fe and fibre intakes were significantly lower in this group.

Conclusions:

School lunches are making a positive contribution to the diets of Swedish children and may mitigate well-established sex differences and social inequalities in dietary intake.
The impact of maternal periodontitis in the development of asthma in the offspring
Authors: Rodrigues Grazielle , Wuo Alexandre do Valle , Klein Simone , de Almeida Patrícia , Damazo Amilcar Sabino , Marcos Rodrigo Labat , Horliana Anna Carolina Ratto Tempestini , Lino-dos-Santos-Franco Adriana
Published: April 2020

Clinical and experimental studies show an association between maternal periodontitis and adverse outcomes during gestation. On the other hand, there were no studies evaluating the impact of maternal periodontitis on the offspring. Thus, our objective was to investigate the repercussion of maternal periodontitis on the development of asthma in the offspring. Pregnant rats were submitted or not to periodontitis by ligature technique. Thirty days after birth, the puppies were sensitized and challenged with ovalbumin (OVA) in order to induce asthmatic response. Our results showed that maternal periodontitis reduced cellular infiltrate in the parenchyma of offspring, tracheal responsiveness, lung edema, and anti-OVA antibodies, without alter mucus as well as cytokines production. We concluded that periodontitis has relevant impact on the offspring’s immune system, blunting the response to allergic and inflammatory stimulus. This study shows the important role of oral health during pregnancy and opens possibilities for future studies in order to explain the effects of periodontitis during pregnancy in the offspring.
DOHaD in low- and middle-income countries: a systematic review exploring gaps in DOHaD population studies
Authors: Tu’akoi Siobhan , Vickers Mark H. , Bay Jacquie L.
Published: April 2020

Low- and middle-income countries (LMICs) are disproportionately affected by non-communicable diseases (NCDs), accounting for more than 80% of NCD-related deaths globally. Research into early-life influences on these diseases via the developmental origins of health and disease (DOHaD) paradigm has informed health promotion interventions and policies focused on optimising early-life health. However, little is known about where this research occurs and whether it reaches and reflects the countries most affected by NCDs. This review searched for DOHaD studies that investigated relationships between factors during pregnancy and at birth, with later-life NCD incidence, risk and related mortality. The aim of this review was to identify where DOHaD research has been conducted and whether this focus is appropriate and relevant, given the differential burden of NCDs. Embase, MEDLINE and Scopus were searched, and eligibility screening processes identified 136 final articles. This review found that 49.7% of DOHaD research was conducted on populations within Western Europe, 15.9% in East Asia, 12.7% in North America, 8.3% in Latin America and the Caribbean, and fewer in Australasia, South Asia, the Middle East, the Africas, and Central Asia. When categorised by income, this review found that 76.4% of studies were based in high-income countries, 19.1% in upper-middle-income and 4.5% in lower-middle-income countries. No studies were based in low-income countries. There is therefore a marked disconnect between where DOHaD research is undertaken and where the greatest NCD disease burden exists. Increasing DOHaD research capacity in LMICs is crucial to informing local strategies that can contribute to reducing the incidence of NCDs.
Crustal Structure of Sri Lanka Derived From Joint Inversion of Surface Wave Dispersion and Receiver Functions Using a Bayesian Approach
Authors: Dreiling Jennifer , Tilmann Frederik , Yuan Xiaohui , Haberland Christian , Seneviratne S. W. Mahinda
Published: April 2020

We study the crustal structure of Sri Lanka by analyzing data from a temporary seismic network deployed in 2016–2017 to shed light on the amalgamation process from a geophysical perspective. Rayleigh wave phase dispersion curves from ambient noise cross correlation and receiver functions were jointly inverted using a transdimensional Bayesian approach. The Moho depths in Sri Lanka range between 30 and 40 km, with the thickest crust (38–40 km) beneath the central Highland Complex (HC). The thinnest crust (30–35 km) is found along the west coast, which experienced crustal thinning associated with the formation of the Mannar Basin. VP/VS ratios lie within a range of 1.60–1.82 and predominantly favor a felsic to intermediate bulk crustal composition with a significant silica content of the rocks. A major intracrustal (18–27 km), slightly westward dipping (∼4.3°) interface with high VS (∼4 km/s) underneath is prominent in the central HC, continuing into the western Vijayan Complex (VC). The discontinuity might have been part of the respective units prior to the collision and could be an indicator for the proposed tilting of the Wanni Complex/HC crustal sections. It might also be related to the deep crustal HC/VC thrust contact with the VC as an indenting promontory of high VS. A low‐velocity zone in the central HC could have been caused by fluid influx generated by the thrusting process.
Fate of Internal Waves on a Shallow Shelf
Authors: Davis Kristen A. , Arthur Robert S. , Reid Emma C. , Rogers Justin S. , Fringer Oliver B. , DeCarlo Thomas M. , Cohen Anne L.
Published: April 2020

Internal waves strongly influence the physical and chemical environment of coastal ecosystems worldwide. We report novel observations from a distributed temperature sensing (DTS) system that tracked the transformation of internal waves from the shelf break to the surf zone over a narrow shelf slope region in the South China Sea. The spatially continuous view of temperature fields provides a perspective of physical processes commonly available only in laboratory settings or numerical models, including internal wave reflection off a natural slope, shoreward transport of dense fluid within trapped cores, and observations of internal rundown (near‐bed, offshore‐directed jets of water preceding a breaking internal wave). Analysis shows that the fate of internal waves on this shelf—whether transmitted into shallow waters or reflected back offshore—is mediated by local water column density structure and background currents set by the previous shoaling internal waves, highlighting the importance of wave‐wave interactions in nearshore internal wave dynamics.
Structure and Seasonal Variation of the Indian Ocean Tropical Gyre Based on Surface Drifters
Authors: Wu Wei , Du Yan , Qian Yu‐Kun , Cheng Xuhua , Wang Tianyu , Zhang Lianyi , Peng Shiqiu
Published: April 2020

Using the Gauss‐Markov decomposition method, this study investigates the mean structure and seasonal variation of the tropical gyre in the Indian Ocean based on the observations of surface drifters. In the climatological mean, the clockwise tropical gyre consists of the equatorial Wyrtki Jets (WJs), the South Equatorial Current (SEC), and the eastern and western boundary currents. This gyre system redistributes the water mass over the entire tropical Indian Ocean basin. Its variations are associated with the monsoon transitions, featuring a typical clockwise pattern in the boreal spring and fall seasons. The relative importance of the geostrophic and Ekman components of the surface currents as well as the role of eddy activity were further examined. It was found that the geostrophic component dominates the overall features of the tropical gyre, including the SEC meandering, the broad eastern boundary current, and the axes of the WJs in boreal spring and fall, whereas the Ekman component strengthens the intensity of the WJs and SEC. Eddies are active over the southeastern tropical Indian Ocean and transport a warm and fresh water mass westward, with direct impact on the southern branch of the tropical gyre. In particular, the trajectories of drifters reveal that during strong Indian Ocean Dipole or El Niño‐Southern Oscillation events, long‐lived eddies were able to reach the southwestern Indian Ocean with a moving speed close to that of the first baroclinic Rossby waves.
Ozone Production in the Soberanes Smoke Haze: Implications for Air Quality in the San Joaquin Valley During the California Baseline Ozone Transport Study
Authors: Langford Andrew O. , Alvarez Raul J. , Brioude J. , Caputi Dani , Conley Stephen A. , Evan S. , Faloona Ian C. , Iraci Laura T. , Kirgis Guillaume , Marrero Josette E. , Ryoo Ju‐Mee , Senff Christoph J. , Yates Emma L.
Published: April 2020

The Soberanes Fire burned 53,470 ha (132,127 acres) along the central California coast between 22 July and 12 October 2016, generating dense smoke and a variety of gaseous compounds that drifted eastward into the San Joaquin Valley Air Basin (SJVAB), an “extreme” nonattainment area for ozone (O3). These gases included nitrogen oxides (NOx) and volatile organic compounds, the photochemical precursors of O3. The fire started during the California Baseline Ozone Transport Study, a field campaign that brought aircraft, surface, and remote sensing measurements of O3 and related species to central California. In this paper, we use the California Baseline Ozone Transport Study measurements to assess the impact of the Soberanes Fire on ozone and particulate air quality in the SJVAB. We focus our analysis on 27 July to 2 August when the smoke haze was heaviest and the highest O3 concentrations in the SJVAB during 2016 were recorded. Our analyses suggest that while 40 to 60 ppbv of fire‐generated O3 was transported to the eastern SJVAB in the 1‐ to 3‐km‐altitude range, relatively little smoke or fire‐generated O3 reached the surface in the Visalia area.
Surface Radiative Flux Bias Reduction Through Regionally Varying Cloud Fraction Parameter Nudging in a Global Coupled Forecast System
Authors: Ridout James A. , Barton Neil P. , Janiga Matthew A. , Reynolds Carolyn A. , May Jackie C. , Rowley Clark , Bishop Craig H.
Published: April 2020

A simple parameter nudging procedure is described that systematically reduces near‐analysis time errors in the surface net shortwave flux in the Navy ESPC (Earth System Prediction Capability) system, a global coupled forecast system that is the product of a continuing development effort at the U. S. Naval Research Laboratory. The procedure generates geographically varying perturbations to one of the cloud fraction parameters in the atmospheric model component of the system during the data assimilation cycle, resulting in large improvements in near‐analysis time surface net shortwave flux biases. After a several week spin‐up period, the global RMSE of the succeeding 10‐day mean bias computed for lead times of 6–12 hours is reduced by 40 percent. Results from application of the approach in a series of 45‐day integrations show that improvements are realized at longer forecast lead times as well. The global RMSE of the surface net shortwave flux averaged over these integrations improves by 37 percent for lead times from 1–5 days, decreasing to 18 percent for lead times from 31–45 days. The corresponding longwave flux errors are slightly degraded, ranging from a 2 percent increase for lead times from 1–5 days to a 0.5 percent increase for lead times from 31–45 days. Global‐mean reductions in ground and sea surface temperature errors are obtained through most of the 45‐day integration period due to improvements over ocean and polar regions. Potential steps for extension and operational application of the method are discussed.
Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis
Authors: Tsukui Tatsuya , Sun Kai-Hui , Wetter Joseph B. , Wilson-Kanamori John R. , Hazelwood Lisa A. , Henderson Neil C. , Adams Taylor S. , Schupp Jonas C. , Poli Sergio D. , Rosas Ivan O. , Kaminski Naftali , Matthay Michael A. , Wolters Paul J. , Sheppard Dean
Published: April 2020

Collagen-producing cells maintain the complex architecture of the lung and drive pathologic scarring in pulmonary fibrosis. Here we perform single-cell RNA-sequencing to identify all collagen-producing cells in normal and fibrotic lungs. We characterize multiple collagen-producing subpopulations with distinct anatomical localizations in different compartments of murine lungs. One subpopulation, characterized by expression of Cthrc1 (collagen triple helix repeat containing 1), emerges in fibrotic lungs and expresses the highest levels of collagens. Single-cell RNA-sequencing of human lungs, including those from idiopathic pulmonary fibrosis and scleroderma patients, demonstrate similar heterogeneity and CTHRC1-expressing fibroblasts present uniquely in fibrotic lungs. Immunostaining and in situ hybridization show that these cells are concentrated within fibroblastic foci. We purify collagen-producing subpopulations and find disease-relevant phenotypes of Cthrc1-expressing fibroblasts in in vitro and adoptive transfer experiments. Our atlas of collagen-producing cells provides a roadmap for studying the roles of these unique populations in homeostasis and pathologic fibrosis.
Timing the initiation of multiple myeloma
Authors: Rustad Even H. , Yellapantula Venkata , Leongamornlert Daniel , Bolli Niccolò , Ledergor Guy , Nadeu Ferran , Angelopoulos Nicos , Dawson Kevin J. , Mitchell Thomas J. , Osborne Robert J. , Ziccheddu Bachisio , Carniti Cristiana , Montefusco Vittorio , Corradini Paolo , Anderson Kenneth C. , Moreau Philippe , Papaemmanuil Elli , Alexandrov Ludmil B. , Puente Xose S. , Campo Elias , Siebert Reiner , Avet-Loiseau Herve , Landgren Ola , Munshi Nikhil , Campbell Peter J. , Maura Francesco
Published: April 2020

The evolution and progression of multiple myeloma and its precursors over time is poorly understood. Here, we investigate the landscape and timing of mutational processes shaping multiple myeloma evolution in a large cohort of 89 whole genomes and 973 exomes. We identify eight processes, including a mutational signature caused by exposure to melphalan. Reconstructing the chronological activity of each mutational signature, we estimate that the initial transformation of a germinal center B-cell usually occurred during the first 2nd-3rd decades of life. We define four main patterns of activation-induced deaminase (AID) and apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) mutagenesis over time, including a subset of patients with evidence of prolonged AID activity during the pre-malignant phase, indicating antigen-responsiveness and germinal center reentry. Our findings provide a framework to study the etiology of multiple myeloma and explore strategies for prevention and early detection.
Nitrogen enrichment increases greenhouse gas emissions from emerged intertidal sandflats
Authors: Hamilton Dallas J. , Bulmer Richard H. , Schwendenmann Luitgard , Lundquist Carolyn J.
Published: April 2020

Unvegetated, intertidal sandflats play a critical role in estuarine carbon and nutrient dynamics. However, these ecosystems are under increasing threat from anthropogenic stressors, especially nitrogen enrichment. While research in this area typically focuses on sediment-water exchanges of carbon and nutrients during tidal inundation, there remain significant gaps in our understanding of GHG (Greenhouse Gas) fluxes during tidal emergence. Here we use in situ benthic chambers to quantify GHG fluxes during tidal emergence and investigate the impact of nitrogen enrichment on these fluxes. Our results demonstrate significant differences in magnitude and direction of GHG fluxes between emerged and submerged flats, demonstrating the importance of considering tidal state when estimating GHG emissions from intertidal flats. These responses were related to differences in microphytobenthic and macrofaunal activity, illustrating the important role of ecology in mediating fluxes from intertidal flats. Our results further demonstrate that nitrogen enrichment of 600 gN m−2 was associated with, on average, a 1.65x increase in CO2 uptake under light (photosynthetically active) conditions and a 1.35x increase in CO2 emission under dark conditions, a 3.8x increase in CH4 emission and a 15x increase in N2O emission overall. This is particularly significant given the large area intertidal flats cover globally, and their increasing exposure to anthropogenic stressors.
Comparison of subjective quality of life after endoscopic submucosal resection or surgery for early gastric cancer
Authors: Tae Chung Hyun , Shim Ki-Nam , Kim Byung-Wook , Kim Jie-Hyun , Hong Su Jin , Baik Gwang Ho , Song Hyun Joo , Kim Yong Sung , Jang Seung-Ho , Jung Hye-Kyung
Published: April 2020

Quality of life (QoL) has become an important issue after early gastric cancer (EGC) treatment. We aimed to compare the QoL of EGC survivors after ESD (n = 241) or laparoscopic subtotal gastrectomy (n = 241) without recurrence and to evaluate the QoL over the 5-year period after adjusting for various confounding factors related to QoL. QoL related to the gastric cancer subscale (GCS) was significantly higher in the ESD group than surgery group (p < 0.001). After adjusting for all possible confounding factors, survivors who underwent ESD still had higher QoL related to CSG than those who underwent surgery. On the analysis of interaction effects for all QoL subscales, higher QoL related to GCS of ESD group than those of surgery group has been kept over time (p = 0.983). Therefore, we concluded that EGC survivors who undergo ESD have significantly better QoL related to GCS over a 5-year period after treatment than those who undergo surgery. This may be a useful consideration when selecting treatment modalities for patients with EGC.
The blue mussel inside: 3D visualization and description of the vascular-related anatomy of Mytilus edulis to unravel hemolymph extraction
Authors: Eggermont Mieke , Cornillie Pieter , Dierick Manuel , Adriaens Dominique , Nevejan Nancy , Bossier Peter , Van den Broeck Wim , Sorgeloos Patrick , Defoirdt Tom , Declercq Annelies Maria
Published: April 2020

The blue mussel Mytilus edulis is an intensely studied bivalve in biomonitoring programs worldwide. The lack of detailed descriptions of hemolymph-withdrawal protocols, particularly with regard to the place from where hemolymph could be perfused from, raises questions regarding the exact composition of aspirated hemolymph and does not exclude the possibility of contamination with other body-fluids. This study demonstrates the use of high resolution X-ray computed tomography and histology combined with 3D-reconstruction using AMIRA-software to visualize some important vascular-related anatomic structures of Mytilus edulis. Based on these images, different hemolymph extraction sites used in bivalve research were visualized and described, leading to new insights into hemolymph collection. Results show that hemolymph withdrawn from the posterior adductor muscle could be extracted from small spaces and fissures between the muscle fibers that are connected to at least one hemolymph supplying artery, more specifically the left posterior gastro-intestinal artery. Furthermore, 3D-reconstructions indicate that puncturing hemolymph from the pericard, anterior aorta, atria and ventricle in a non-invasive way should be possible. Hemolymph withdrawal from the heart is less straightforward and more prone to contamination from the pallial cavity. This study resulted simultaneously in a detailed description and visualization of the vascular-related anatomy of Mytilus edulis.
Increased static dielectric constant in ZnMnO and ZnCoO thin films with bound magnetic polarons
Authors: Vegesna Sahitya V. , Bhat Vinayak J. , Bürger Danilo , Dellith Jan , Skorupa Ilona , Schmidt Oliver G. , Schmidt Heidemarie
Published: April 2020

A novel small signal equivalent circuit model is proposed in the inversion regime of metal/(ZnO, ZnMnO, and ZnCoO) semiconductor/Si3N4 insulator/p-Si semiconductor (MSIS) structures to describe the distinctive nonlinear frequency dependent capacitance (C-F) and conductance (G-F) behaviour in the frequency range from 50 Hz to 1 MHz. We modelled the fully depleted ZnO thin films to extract the static dielectric constant (εr) of ZnO, ZnMnO, and ZnCoO. The extracted enhancement of static dielectric constant in magnetic n-type conducting ZnCoO (εr ≥ 13.0) and ZnMnO (εr ≥ 25.8) in comparison to unmagnetic ZnO (εr = 8.3–9.3) is related to the electrical polarizability of donor-type bound magnetic polarons (BMP) in the several hundred GHz range (120 GHz for CdMnTe). The formation of donor-BMP is enabled in n-type conducting, magnetic ZnO by the s-d exchange interaction between the electron spin of positively charged oxygen vacancies $${V}_{o}^{+}$$Vo+ in the BMP center and the electron spins of substitutional Mn2+ and Co2+ ions in ZnMnO and ZnCoO, respectively. The BMP radius scales with the Bohr radius which is proportional to the static dielectric constant. Here we show how BMP overlap can be realized in magnetic n-ZnO by increasing its static dielectric constant and guide researchers in the field of transparent spintronics towards ferromagnetism in magnetic, n-ZnO.
Mediators of the association between low socioeconomic status and poor glycemic control among type 2 diabetics in Bangladesh
Authors: Rahman Mosiur , Nakamura Keiko , Hasan S. M. Mahmudul , Seino Kaoruko , Mostofa Golam
Published: April 2020

Although low socioeconomic status (SES) is related to poor glycemic control, the underlying mechanisms remain unclear. We examined potentially modifiable factors involved in the association between low SES and poor glycemic control using data from the baseline survey of a multicenter, prospective cohort study. Five hundred adult type 2 diabetes patients were recruited from three diabetes centers. Glycemic control was poorer in diabetic individuals with low SES than in those with higher SES. Adverse health-related behaviors, such as non-adherence to medication (adjusted odds ratio [AOR] = 1.07, 95% confidence interval [CI] 1.04–1.13) and diet (AOR = 1.04, 95% CI 1.02–1.06); existing comorbidities, such as depressive symptoms (AOR = 1.05, 95% CI 1.04–1.09); and non-adherence to essential health service-related practices concerning diabetes care, such as irregular scheduled clinic visits (AOR = 1.04, 95% CI 1.03–1.06) and not practicing self-monitoring of blood glucose (AOR = 1.05, 95% CI 1.03–1.07), mediated the relationship between social adversity and poor glycemic control specially in urban areas of Bangladesh. Those identified factors provide useful information for developing interventions to mitigate socioeconomic disparities in glycemic control.
Climatic and societal impacts of a “forgotten” cluster of volcanic eruptions in 1108-1110 CE
Authors: Guillet Sébastien , Corona Christophe , Ludlow Francis , Oppenheimer Clive , Stoffel Markus
Published: April 2020

Recently revised ice core chronologies for Greenland have newly identified one of the largest sulfate deposition signals of the last millennium as occurring between 1108 and 1113 CE. Long considered the product of the 1104 CE Hekla (Iceland) eruption, this event can now be associated with substantial deposition seen in Antarctica under a similarly revised chronology. This newly recognized bipolar deposition episode has consequently been deemed to reveal a previously unknown major tropical eruption in 1108 CE. Here we show that a unique medieval observation of a “dark” total lunar eclipse attests to a dust veil over Europe in May 1110 CE, corroborating the revised ice-core chronologies. Furthermore, careful evaluation of ice core records points to the occurrence of several closely spaced volcanic eruptions between 1108 and 1110 CE. The sources of these eruptions remain unknown, but we propose that Mt. Asama, whose largest Holocene eruption occurred in August 1108 CE and is credibly documented by a contemporary Japanese observer, is a plausible contributor to the elevated sulfate in Greenland. Dendroclimatology and historical documentation both attest, moreover, to severe climatic anomalies following the proposed eruptions, likely providing the environmental preconditions for subsistence crises experienced in Western Europe between 1109 and 1111 CE.
Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel
Authors: Ramadhan Wahyu , Kagawa Genki , Moriyama Kousuke , Wakabayashi Rie , Minamihata Kosuke , Goto Masahiro , Kamiya Noriho
Published: April 2020

In this report, a strategy for constructing three-dimensional (3D) cellular architectures comprising viable cells is presented. The strategy uses a redox-responsive hydrogel that degrades under mild reductive conditions, and a confluent monolayer of cells (i.e., cell sheet) cultured on the hydrogel surface peels off and self-folds to wrap other cells. As a proof-of-concept, the self-folding of fibroblast cell sheet was triggered by immersion in aqueous cysteine, and this folding process was controlled by the cysteine concentration. Such folding enabled the wrapping of human hepatocellular carcinoma (HepG2) spheroids, human umbilical vein endothelial cells and collagen beads, and this process improved cell viability, the secretion of metabolites and the proliferation rate of the HepG2 cells when compared with a two-dimensional culture under the same conditions. A key concept of this study is the ability to interact with other neighbouring cells, providing a new, simple and fast method to generate higher-order cellular aggregates wherein different types of cellular components are added. We designated the method of using a cell sheet to wrap another cellular aggregate the ‘cellular Furoshiki’. The simple self-wrapping Furoshiki technique provides an alternative approach to co-culture cells by microplate-based systems, especially for constructing heterogeneous 3D cellular microstructures.
α-synuclein inclusions are abundant in non-neuronal cells in the anterior olfactory nucleus of the Parkinson’s disease olfactory bulb
Authors: Stevenson Taylor J. , Murray Helen C. , Turner Clinton , Faull Richard L. M. , Dieriks Birger V. , Curtis Maurice A.
Published: April 2020

Reduced olfactory function (hyposmia) is one of the most common non-motor symptoms experienced by those living with Parkinson’s disease (PD), however, the underlying pathology of the dysfunction is unclear. Recent evidence indicates that α-synuclein (α-syn) pathology accumulates in the anterior olfactory nucleus of the olfactory bulb years before the motor symptoms are present. It is well established that neuronal cells in the olfactory bulb are affected by α-syn, but the involvement of other non-neuronal cell types is unknown. The occurrence of intracellular α-syn inclusions were quantified in four non-neuronal cell types – microglia, pericytes, astrocytes and oligodendrocytes as well as neurons in the anterior olfactory nucleus of post-mortem human PD olfactory bulbs (n = 11) and normal olfactory bulbs (n = 11). In the anterior olfactory nucleus, α-syn inclusions were confirmed to be intracellular in three of the four non-neuronal cell types, where 7.78% of microglia, 3.14% of pericytes and 1.97% of astrocytes were affected. Neurons containing α-syn inclusions comprised 8.60% of the total neuron population. Oligodendrocytes did not contain α-syn. The data provides evidence that non-neuronal cells in the PD olfactory bulb contain α-syn inclusions, suggesting that they may play an important role in the progression of PD.
Retinal phototoxicity and the evaluation of the blue light hazard of a new solid-state lighting technology
Authors: Jaadane Imene , Villalpando Rodriguez Gloria , Boulenguez Pierre , Carré Samuel , Dassieni Irene , Lebon Cecile , Chahory Sabine , Behar-Cohen Francine , Martinsons Christophe , Torriglia Alicia
Published: April 2020

Exposure Limit Values (ELV) for artificial lighting were defined in order to prevent light-induced damage to the retina. The evaluation of the lighting devices include the correction of their spectra by the B(λ) function or blue light hazard function, representing the relative spectral sensitivity of the human eye to the blue light. This weighting function peaks between 435 and 440 nm. In this study we evaluate a new generation of light emitting diode (LED), the GaN-on-GaN (gallium nitride on gallium nitride) LED, that present an emission peak in the purple part of the spectrum. Wistar rats were exposed to GaN-on-GaN and conventional diodes at different retinal doses (from 2.2 to 0.5 J/cm2). We show that GaN-on-GaN diodes are more toxic than conventional LED for the rat neural retina and the rat retinal pigment epithelium, indicating that the BLH (blue light hazard) weighting is not adapted to this type of diodes. One of the reasons of this increased toxicity is the effects of shorter wavelengths on mitochondria polarization. We also show that the threshold of phototoxic retinal dose in the rat (fixed at 11 J/cm2, BLH weighted) is overestimated, suggesting that the values used for regulations, calculated in primates using the same methods than in rats, should be revised.
Identification and molecular characterization of the first complete genome sequence of Human Parechovirus type 15
Authors: Fernandez-Garcia Maria Dolores , Simon-Loriere Etienne , Kebe Ousmane , Sakuntabhai A. , Ndiaye Kader
Published: April 2020

Using a metagenomics approach, we have determined the first full-length genome sequence of a human parechovirus type 15 (HPeV15) strain, isolated from a child with acute flaccid paralysis and co-infected with EV-A71. HPeV15 is a rarely reported type. To date, no full-length genome sequence of HPeV15 is available in the GenBank database, where only limited VP1 sequences of this virus are available. Pairwise comparisons of the complete VP1 nucleotide and deduced amino acid sequences revealed that the study strain belongs to type 15 as it displayed 79.6% nucleotide and 93.4% amino acid identity with the HPeV15 prototype strain. Comparative analysis of available genomic regions and phylogenetic analysis using the P2 and P3 coding regions revealed low nucleotide identity to HPeV reference genomes. Phylogenetic and similarity plot analyses showed that genomic recombination events might have occurred in the UTRs and nonstructural region during HPeV15 evolution. The study strain has high similarity features with different variants of HPeV3 suggesting intertypic recombination. Our data contributes to the scarce data available on HPeVs in Africa and provides valuable information for future studies that aim to understand the evolutionary history, molecular epidemiology or biological and pathogenic properties of HPeV15.
Data-driven segmentation of audiometric phenotypes across a large clinical cohort
Authors: Parthasarathy Aravindakshan , Romero Pinto Sandra , Lewis Rebecca M. , Goedicke William , Polley Daniel B.
Published: April 2020

Pure tone audiograms are used to assess the degree and underlying source of hearing loss. Audiograms are typically categorized into a few canonical types, each thought to reflect distinct pathologies of the ear. Here, we analyzed 116,400 patient records from our clinic collected over a 24-year period and found that standard categorization left 46% of patient records unclassified. To better account for the full spectrum of hearing loss profiles, we used a Gaussian Mixture Model (GMM) to segment audiograms without any assumptions about frequency relationships, interaural symmetry or etiology. The GMM converged on ten types, featuring varying degrees of high-frequency hearing loss, flat loss, mixed loss, and notched profiles, with predictable relationships to patient age and sex. A separate GMM clustering of 15,380 audiograms from the National Health and Nutrition Examination Survey (NHANES) identified six similar types, that only lacked the more extreme hearing loss configurations observed in our patient cohort. Whereas traditional approaches distill hearing loss configurations down to a few canonical types by disregarding much of the underlying variability, an objective probabilistic model that accounted for all of the data identified an organized, but more heterogenous set of audiogram types that was consistent across two large clinical databases.
Single crystal growth, optical absorption and luminescence properties under VUV-UV synchrotron excitation of type III Pr3+:KGd(PO3)4
Authors: Adell Irina , Pujol Maria Cinta , Solé Rosa Maria , Lancry Matthieu , Ollier Nadège , Aguiló Magdalena , Díaz Francesc
Published: April 2020

Scintillator materials are widely used for a variety of applications such as high energy physics, astrophysics and medical imaging. Since the ideal scintillator does not exist, the search for scintillators with suitable properties for each application is of great interest. Here, Pr3+-doped KGd(PO3)4 bulk single crystals with monoclinic structure (space group: P21) are grown from high temperature solutions and their structural, thermal and optical properties are studied as possible candidates for scintillation material. The change in the unit cell parameters as a function of the Pr3+ level of doping and temperature is studied. Differential thermal analysis reveals that KGd0.942Pr0.058(PO3)4 is stable until 1140 K. The 5d3, 5d2 and 5d1 levels of Pr3+ with respect to the 3H4 ground state are centred at 166, 196 and 218 nm, respectively, in this host. The luminescence of KGd0.990Pr0.010(PO3)4, by exciting these 5d levels, shows intense emissions centred at 256 and 265 nm from the 5d1 to 3F3,4 and 1G4 levels of Pr3+ with a short decay time of 6 ns. The 6P3/2,5/2,7/2 → 8S7/2 transitions of Gd3+ appear after exciting the 5d levels of Pr3+ and the 4 f levels of Gd3+, showing an energy transfer between Pr3+ and Gd3+.
Extended fed-batch fermentation of a C5/C6 optimised yeast strain on wheat straw hydrolysate using an online refractive index sensor to measure the relative fermentation rate
Authors: Knudsen Jan Dines , Rønnow Birgitte
Published: April 2020

In the production of 2nd generation ethanol, using Saccharomyces cerevisiae, the highest productivity obtained using C5/C6 fermenting yeast is in the co-fermentation phase, in which xylose and glucose are fermented simultaneously. Extending this phase in a fed-batch process increases the yield, rate and additionally reduces needed yeast amount for pitching. Extending this phase, as long as possible, would further enhance yield and economy of the process. To realise the concept a fermentation monitoring technique was developed and applied. Based on online measured refractive index an optimal residual sugar concentration could be maintained in the primary fermentor during the feed phase, requiring little knowledge of the nature of the substrate. The system was able to run stably for at least five fermentor volumes giving an ethanol yield >90% throughout the run. This was achieved with addition of only urea to the wheat straw hydrolysate and with an initial yeast pitch of 0.2 g/L total of finished broth. It has the potential to improve the fermentation technology used in fuel ethanol plants, which could help to meet the growing demand for more sustainable fuels.
Impulsivity is a heritable trait in rodents and associated with a novel quantitative trait locus on chromosome 1
Authors: Jupp Bianca , Pitzoi Silvia , Petretto Enrico , Mar Adam C. , Oliver Yolanda Pena , Jordan Emily R. , Taylor Stephanie , Atanur Santosh S. , Srivastava Prashant K. , Saar Kathrin , Hubner Norbert , Sommer Wolfgang H. , Staehlin Oliver , Spanagel Rainer , Robinson Emma S. , Schumann Gunter , Moreno Margarita , Everitt Barry J. , Robbins Trevor W. , Aitman Timothy J. , Dalley Jeffrey W.
Published: April 2020

Impulsivity describes the tendency to act prematurely without appropriate foresight and is symptomatic of a number of neuropsychiatric disorders. Although a number of genes for impulsivity have been identified, no study to date has carried out an unbiased, genome-wide approach to identify genetic markers associated with impulsivity in experimental animals. Herein we report a linkage study of a six-generational pedigree of adult rats phenotyped for one dimension of impulsivity, namely premature responding on the five-choice serial reaction time task, combined with genome wide sequencing and transcriptome analysis to identify candidate genes associated with the expression of the impulsivity trait. Premature responding was found to be heritable (h2 = 13–16%), with significant linkage (LOD 5.2) identified on chromosome 1. Fine mapping of this locus identified a number of polymorphic candidate genes, however only one, beta haemoglobin, was differentially expressed in both the founder strain and F6 generation. These findings provide novel insights into the genetic substrates and putative neurobiological mechanisms of impulsivity with broader translational relevance for impulsivity-related disorders in humans.
Association between milk consumption and child growth for children aged 6–59 months
Authors: Herber Christine , Bogler Lisa , Subramanian S. V. , Vollmer Sebastian
Published: April 2020

Apart from high levels of energy, proteins, micro- and macronutrients, milk contains calcium and the insulin-like growth factor-1 that are of major relevance for children’s development and growth. Using Demographic and Health Survey data between 1990 and 2017 with information on milk consumption and anthropometric measurements from all low- and middle-income countries available, we investigate whether milk consumption in childhood is associated with stunting, wasting, and underweight. We specify logistic regression models and adjust for a range of covariates and fixed effects on the primary sampling unit level. We analyze heterogeneity in the association by wealth quintiles and age groups and present country-specific estimates. The final samples for wasting, underweight and stunting include 668.463, 693.376, and 673.177 observations of children aged 6 to 59 months, respectively. Our results suggest that milk consumption is associated with a reduced probability of being underweight of 1.4 percentage points (95% confidence interval −0.02, −0.01) and a reduced probability of being stunted of 1.9 percentage points (95% confidence interval −0.02, −0.01). The association for wasting is not robust. The association is stronger for children from wealthier households, which might indicate that milk consumption is a proxy for better overall nutrition or socio-economic status.
Relationship between lumbar lordosis and the ratio of the spinous process height to the anterior spinal column height
Authors: Inanami Hirohiko , Iwai Hiroki , Kaneko Takeshi , Oshina Masahito , Manabe Nodoka , Takano Yuichi , Yuzawa Yohei , Segawa Tomohide , Yanagisawa Kazuyoshi , Hirai Shima , Saiki Fumiko , Fukushima Masayoshi , Oka Hiroyuki , Matsudaira Ko , Oshima Yasushi , Koga Hisashi
Published: April 2020

Purpose Global sagittal imbalance with lumbar hypo-lordosis leads to various problems in elderly populations and is often treated with long-segment fusion and osteotomy. These highly invasive procedures result in a wide range of rigid spines with a high rate of complications. Although some reports have mentioned the primary aetiology of hypo-lordosis, there is limited evidence. Thus, understanding the exact underlying mechanism is required for developing minimally invasive procedures. This study aimed to investigate the factors related to lumbar lordosis (LL) in elderly people. Methods A total of ninety consecutive patients aged ≥ sixty years at a single spine centre were included. We measured LL, the anterior spinal column height consisting of vertebral bodies and intervertebral discs from L1 to L5 (ASC-5) and the sum of the spinous process heights from L1 to L5 (SP-5) with computed tomography in a supine position. The relationship between LL and the SP-5/ASC-5 ratio, SP-5, and ASC-5 was analysed. Results The Pearson correlation coefficients between LL and the SP-5/ASC-5 ratio, SP-5, and ASC-5 were −0.80 (p < 0.001), −0.43 (p < 0.001) and 0.36 (p < 0.001), respectively. Conclusion LL was significantly related to the SP-5/ASC-5 ratio of the lumbar spine in elderly people. In addition to shortening of the ASC, elongation of the SP-5 also contributed to a loss of LL.