bluuebunny/crossref_metadata_2025 huggingface dataset:
Microscopy and Microanalysis of Reverse-Osmosis and Nanofiltration Membranes
Authors: Cahill David G. , Freger Viatcheslav , Kwak Seung-Yeop
Published: February 2011

The polyamide active layers of commercial reverse-osmosis and nanofiltration membranes are examples of nanoscale functional materials that challenge the state of the art of materials characterization. The active layer is only ∼100 nm thick, and because the active layer is formed by a process of interfacial polymerization, the structure and composition of the membrane is highly inhomogeneous. Even such basic physical and chemical properties of the membrane as the atomic density, swelling in water, distribution of charged species, and the mobility of water and ions, are poorly understood. In this article, we briefly review progress in the characterization of polyamide separation membranes using transmission electron microscopy, atomic force microscopy, vibrational spectroscopy, positron annihilation, nuclear magnetic resonance, and Rutherford backscattering spectrometry. Advances in the microanalysis methods applicable to these complex materials will advance fundamental understanding of the structure–property relationships of polymer membranes and further the long-term goal of synthesizing membranes with improved performance.
Three-Dimensional Materials Science: An Intersection of Three-Dimensional Reconstructions and Simulations
Authors: Thornton Katsuyo , Poulsen Henning Friis
Published: February 2011

The recent development of experimental techniques that rapidly reconstruct the three-dimensional microstructures of solids has given rise to new possibilities for developing a deeper understanding of the evolution of microstructures and the effects of microstructures on materials properties. Combined with three-dimensional (3D) simulations and analyses that are capable of handling the complexity of these microstructures, 3D reconstruction, or tomography, has become a powerful tool that provides clear insights into materials processing and properties. This introductory article provides an overview of this emerging field of materials science, as well as brief descriptions of selected methods and their applicability.
Accessing Inaccessible Interfaces: In Situ Approaches to Materials Tribology
Authors: Sawyer W. Gregory , Wahl Kathryn J.
Published: February 2011

The field of materials tribology has entered a phase of instrumentation and measurement that involves accessing and following the detailed chemical, structural, and physical interactions that govern friction and wear. Fundamental tribological research involves the development of new experimental methods capable of monitoring phenomena that occur within the life of a sliding contact. Measuring friction phenomena while the process is ongoing is a major improvement over earlier techniques that required the surfaces to be separated and analyzed, thereby interrupting the friction-causing event and modifying surface conditions. In the past, MRS Bulletin has highlighted how in situ approaches can greatly enhance our understanding of materials structure, processing, and performance. This issue highlights in situ approaches as applied to materials tribology, namely, the study of contacting surfaces and interfaces in relative motion.
Fundamental Studies of Nanometer-Scale Wear Mechanisms
Authors: Bennewitz R. , Dickinson J.T.
Published: February 2011

Fundamental processes of wear include the rupture of single chemical bonds and the displacement of atoms or small clusters by mechanical action. Experimental studies of such processes have become feasible with the development of scanning probe microscopy. The small volume affected in these experiments overlaps with the size scale of large atomistic simulations, making a direct comparison possible. The complexity of real-world wear processes is reduced in most nanometer-scale experiments, for example, by probing surfaces of single crystals or by establishing and maintaining carefully controlled environments, including ultraclean conditions. The studies address the onset and topography of wear, the formation of debris structures, the interplay of mechanical and chemical action, the role of ultrathin films, the role of crystal defects in wear processes, and temporal and thermal effects.
Carbon Nanotube Electroactive Polymer Materials: Opportunities and Challenges
Authors: Qu Liangti , Peng Qiang , Dai Liming , Spinks Geoffrey M. , Wallace Gordon G. , Baughman Ray H.
Published: February 2011

Carbon nanotubes (CNTs) with macroscopically ordered structures (e.g., aligned or patterned mats, fibers, and sheets) and associated large surface areas have proven promising as new CNT electroactive polymer materials (CNT-EAPs) for the development of advanced chemical and biological sensors. The functionalization of CNTs with many biological species to gain specific surface characteristics and to facilitate electron transfer to and from them for chemical- and bio-sensing applications is an area of intense research activity.

Mechanical actuation generated by CNT-EAPs is another exciting electroactive function provided by these versatile materials. Controlled mechanical deformation for actuation has been demonstrated in CNT mats, fibers, sheets, and individual nanotubes. This article summarizes the current status and technological challenges for the development of electrochemical sensors and electromechanical actuators based on carbon nanotube electroactive materials.
Factors That Will Influence Oil and Gas Supply and Demand in the 21st Century
Authors: Holditch Stephen A. , Chianelli Russell R.
Published: February 2011

A recent report published by the National Petroleum Council (NPC) in the United States predicted a 50–60% growth in total global demand for energy by 2030. Because oil, gas, and coal will continue to be the primary energy sources during this time, the energy industry will have to continue increasing the supply of these fuels to meet this increasing demand. Achieving this goal will require the exploitation of both conventional and unconventional reservoirs of oil and gas in an environmentally acceptable manner. Such efforts will, in turn, require advancements in materials science, particularly in the development of materials that can withstand high-pressure, high-temperature, and high-stress conditions.
Solar Energy Conversion Toward 1 Terawatt
Authors: Ginley David , Green Martin A. , Collins Reuben
Published: February 2011

The direct conversion of solar energy to electricity by photovoltaic cells or thermal energy in concentrated solar power systems is emerging as a leading contender for next-generation green power production. The photovoltaics (PV) area is rapidly evolving based on new materials and deposition approaches. At present, PV is predominately based on crystalline and polycrystalline Si and is growing at >40% per year with production rapidly approaching 3 gigawatts/year with PV installations supplying <1% of energy used in the world. Increased cell efficiency and reduced manufacturing expenses are critical in achieving reasonable costs for PV and solarthermal. CdTe thin-film solar cells have reported a manufactured cost of $1.25/watt. There is also the promise of increased efficiency by use of multijunction cells or hybrid devices organized at the nanoscale. This could lead to conversion efficiencies of greater than 50%. Solar energy conversion increasingly represents one of the largest new businesses currently emerging in any sector of the economy.
Engineered and Artificial Photosynthesis: Human Ingenuity Enters the Game
Authors: Gust Devens , Kramer David , Moore Ana , Moore Thomas A. , Vermaas Wim
Published: February 2011

All oxygen-dependent life depends on photosynthesis. In addition to breathing the oxygen produced by photosynthesis, humans have been harnessing energy from photosynthesis for millennia. Since the beginning of human societal structures, human needs have driven the evolution of agricultural production, and they continue to do so. Recently, it has been suggested that agriculture can contribute substantially to human technological (nonnutritional) energy needs. This possibility raises concern because the projections of human energy needs argue convincingly that without large increases in energy conversion effciency (ECE), land-grown biofuel production and food production will compete for land, a largely untenable compromise given the current nutritional status of the world's underdeveloped societies.
Road Transportation Vehicles
Authors: Carpenter Joseph A. , Gibbs Jerry , Pesaran Ahmad A. , Marlino Laura D. , Kelly Kenneth
Published: February 2011

In many industrial countries, road transportation accounts for a significant portion of the country's energy consumption. In developing countries, the use of energy for transportation is on the rise. The recent increase in petroleum prices, expanding world economic prosperity, the probable peaking of conventional petroleum production in the coming decades, and concerns about global climate changes require efforts to increase the efficiency of the use of, and develop alternatives for, petroleum-based fuels used in road transportation. The energy efficiency of a vehicle could be improved in several ways: lightweighting the vehicle structure and powertrain using advanced materials and designs, improving the efficiency of the internal combustion engine, reducing tire rolling resistance, and hybridization. Each of these efforts will require improvements in materials and processes.
Solid-State Lighting
Authors: Humphreys Colin J.
Published: February 2011

Electricity generation is the main source of energy-related greenhouse gas emissions and lighting uses one-fifth of its output. Solid-state lighting using light-emitting diodes (LEDs) is poised to reduce this value by at least 50%, so that lighting will then use less than one-tenth of all electricity generated. LED lighting will provide reductions of at least 10% in fuel consumption and carbon dioxide emissions from power stations within the next 5–10 years. Even greater reductions are likely on a 10–20-year timescale.
Nanoporous Metals for Catalytic and Optical Applications
Authors: Ding Yi , Chen Mingwei
Published: February 2011

Nanoporous metals (NPMs) made by dealloying represent a class of functional materials with the unique structural properties of mechanical rigidity, electrical conductivity, and high corrosion resistance. They also possess a porous network structure with feature dimensions tunable within a wide range from a few nanometers to several microns. Coupled with a rich surface chemistry for further functionalization, NPMs have great potential for applications in heterogeneous catalysis, electrocatalysis, fuel cell technologies, biomolecular sensing, surface-enhanced Raman scattering (SERS), and plasmonics. This article summarizes recent advances in some of these areas and, in particular, we focus on the discussion of microstructure, catalytic, and optical properties of nanoporous gold (NPG). With advanced electron microscopy, three-dimensional tomographic reconstructions of NPG have been realized that yield quantitative characterizations of key morphological parameters involved in the intricate structure. Catalytic and electrocatalytic investigations demonstrate that bare NPG is already catalytically active for many important reactions such as CO and glucose oxidation. Surface functionalization with other metals, such as Pt, produces very efficient electrocatalysts, which have been used as promising fuel cell electrode materials with very low precious metal loading. Additionally, NPG and related materials possess outstanding optical properties in plasmonics and SERS. They hold promise to act as highly active, stable, and economically affordable substrates in high-performance instrumentation applications for chemical inspection and biomolecular diagnostics. Finally, we conclude with some perspectives that appear to warrant future investigation.
Proton Conduction in Solids: Bulk and Interfaces
Authors: Norby Truls
Published: February 2011

Truly proton-conducting materials would have an immense impact on sustainable energy technologies for the 21st century, through efficient fuel cells, electrolyzers, and gas-separation membranes. However, proton conduction combined with materials stability seems difficult to achieve, and some hurdles and pathways are outlined in this article. Problems, possibilities, and artifacts of transport across and along interfaces are discussed, linked mainly to space-charge layer properties and engineering of the grain-boundary core and to water in nanovoids. The importance of protons in many semiconducting functional oxides is also explained. At lower temperatures and in humid environments, the presence of protonated cation vacancies (Ruetschi defects) is predicted and is expected to play an important role in photoelectrochemistry, catalysis, and surface transport.
Nonpolar/Semipolar GaN Technology for Violet, Blue, and Green Laser Diodes
Authors: Ohta Hiroaki , Okamoto Kuniyoshi
Published: February 2011

To achieve 520–532 nm green laser diodes (LDs), nonpolar and semipolar nitrides have attracted much attention because their usage leads to the elimination of the quantum-confined Stark effect and higher optical gains in this wavelength region. Since the breakthrough in the homoepitaxial growth technology for them, many nonpolar m -plane devices such as mW-class blue light-emitting diodes, violet 405 nm LDs, blue 460 nm LDs, and blue-green LDs beyond 490 nm have been announced. Advantages such as small blueshift and high slope efficiency (high output power to injected current ratio) have been confirmed for the first time in m -plane LDs beyond the blue region. On the other hand, the semipolar plane is also a candidate for green LDs. The pulsed operation of semipolar (1011) and (1122) violet LDs and lasing for a (1122) LD at 514 nm by optical pumping also have been reported. Such rapid progress in this research field will be reviewed.
In Situ Mechanical Testing of Biological and Inorganic Materials at the Micro- and Nanoscales
Authors: Eberl C. , Saif T.
Published: February 2011

A central goal of materials science is to reveal how a material deforms under mechanical stress and how the deformation is related to its microstructure. This goal is best achieved by “seeing” the evolving microstructure when the property is measured quantitatively. Mechanical testing methods have thus evolved over time to test materials at the micro- and nanoscale while observing the changes in the specimen. Recent advances in microtechnology offer a new generation of microscale sensors and actuators that allow in situ studies of both living and nonliving materials in analytical instruments. Such experiments are providing new and fundamental insights on the structure-property relations in materials and revealing remarkable links between the mechanical properties of living cells and their functions. This issue presents five articles that discuss state-of-the-art methodologies for in situ mechanical tests and highlights new findings through the use of these techniques.
Energy and expansion: The Tata story
Authors: Arunachalam V. S. , Rao Gopal R.
Published: February 2011

Tata is a household name in India. Starting well over a century ago, the Tatas began their foray into industry, and the brand represents more than a diversifed conglomerate. The Tatas are well-respected, having endeared themselves in every walk of Indian life. Their businesses are all-pervasive, from building automobiles and generating electric power to making steel and building supercomputers. Their charitable trusts and endowments are as extensive as their businesses. They have founded and funded outstanding educational institutions and human welfare organizations, and they have supported research on alleviating human suffering. The incumbent of the Tata Group is Ratan Naval Tata, a Cornell University graduate in architecture. In the 20 years since he took over the mantle, he has set the Tata industries on a steep growth trajectory, increasing the revenue of the Tata industries 12-fold, making automobiles—the famed Nano—available at very affordable prices in India, and introducing effcient steelmaking. The business empire he heads, the Tata Group, has over 90 companies with footholds in 80 countries. In the midst of his busy globe-trotting schedule, we managed to steal an hour of his time at the Tatas' “Bombay House” headquarters for him to tell us how he sees the global energy challenges and the opportunities they create.
New surprises “down below”: Recent successes in the synthesis of actinide materials
Authors: Sarrao J. L. , Haga Y. , Ward R. C. C.
Published: February 2011

Recent discoveries of novel electronic states, including relatively high-temperature superconductivity, in the actinides point to exciting prospects for future discoveries at the bottom of the periodic table. A key ingredient in all of the successes discussed here is the role of high-quality synthesis in enabling advances. Results on PuCoGa51, NpPd5Al2, and single crystal uranium are discussed.
The Role of RANK-Ligand Inhibition in Cancer: The Story of Denosumab
Authors: Castellano Daniel , Sepulveda Juan Manuel , García-Escobar Ignacio , Rodriguez-Antolín Alfredo , Sundlöv Anna , Cortes-Funes Hernán
Published: February 2011

The diagnosis of bone metastases is an event with certain consequences for the patient. They often mean pain and can also mean pathological fractures, hypercalcemia, and spinal cord compression, all synonymous with a diminished quality of life and often also hospitalization. Since the advent of the intravenous bisphosphonates, things began to look a bit brighter for patients with bone metastases—bone destruction was kept at bay a little longer. The next generation of bone metastasis treatments is well on its way in clinical development, and among them, the most advanced drug is denosumab. Denosumab is a fully human monoclonal antibody that inhibits osteoclast maturation, activation, and function by binding to receptor activator of nuclear factor kappa B ligand, with the final result being a reduced rate of bone resorption. In this review, we give an overview of relevant preclinical and clinical data regarding the use of denosumab in patients with solid tumors in general and prostate cancer in particular.
A Systematic Review and Meta-Analysis of Intravenous Palonosetron in the Prevention of Chemotherapy-Induced Nausea and Vomiting in Adults
Authors: Likun Zhou , Xiang Jing , Yi Ba , Xin Duan , Tao Zheng Liu
Published: February 2011

Objectives.

We performed a systematic review and meta-analysis to compare treatment effectiveness and adverse effects in cancer patients receiving chemotherapy with palonosetron to prevent chemotherapy-induced nausea and vomiting (CINV).

Methods.

We identified randomized controlled clinical trials (RCT) comparing palonosetron with first-generation 5-HT3RA in the prevention of CINV in cancer patients. Meta-analyses were performed on homogeneous studies. Fixed or random-effects models were used to combine data.

Results.

Eight eligible trials were identified, reporting outcomes on 3,592 patients. Meta-analyses showed statistically significant differences in favor of palonosetron compared with first-generation 5-HT3RA in prevention of acute CINV (p = .0003), delayed CINV (p < .00001), and overall phase of CINV (p < .00001). Subgroup analyses showed statistically significant differences in favor of both 0.25 mg and 0.75 mg of palonosetron in prevention of all phases of CINV. There were no statistically significant differences between 0.25 and 0.75 mg of palonosetron. Compared with the first-generation 5-HT3RA, 0.75 mg of palonosetron showed a statistically significant difference in the occurrence of constipation (p = .04).

Interpretation.

The use of palonosetron should be considered an integral part of adjuvant therapy for prevention of the acute, delayed, and overall phases of CINV. The 0.25 mg intravenous palonosetron dose is as effective as the 0.75 mg intravenous palonosetron dose. However, 0.75 mg intravenous palonosetron causes constipation more frequently than the first-generation 5-HT3RA.
Research frontiers in the analysis of coupled biogeochemical cycles
Authors: Finzi Adrien C , Cole Jonathan J , Doney Scott C , Holland Elisabeth A , Jackson Robert B
Published: February 2011

The analysis of coupled biogeochemical cycles (CBCs) addresses the scientific basis for some of today's major environmental problems. Drawing from information presented at a series of sessions on CBCs held at the 2009 Annual Meeting of the Ecological Society of America and from the research community's expertise, we identify several principal research themes that justify action and investment. Critical areas for research include: coupling of major element cycles to less studied yet equally important trace element cycles; analyzing CBCs across ecosystem boundaries; integrating experimental results into regional‐ and global‐scale models; and expanding the analysis of human interactions with CBCs arising from human population growth, urbanization, and geoengineering. To advance the current understanding of CBCs and to address the environmental challenges of the 21st century, scientists must maintain and synthesize data from existing observational and experimental networks, develop new instrumentation networks, and adopt emerging technologies.
The Accuracy of Identifying the Greater Palatine Neurovascular Bundle: A Cadaver Study
Authors: Fu Jia‐Hui , Hasso Dawlat G. , Yeh Chu‐Yuan , Leong Daylene J.M. , Chan Hsun‐Liang , Wang Hom‐Lay
Published: February 2011

Background: The palate is a common site for harvesting subepithelial connective tissue grafts (SCTG). The size of SCTG that can be harvested is dictated by the position of the greater palatine neurovascular bundle (GPB). The aims of this cadaver study are to assess the accuracy of predicting the location of the GPB on study models and to evaluate anatomic factors that might influence the predictability.

Methods: Eleven fully dentate or partially edentulous maxillary cadavers were used. Study models were fabricated after the greater palatine foramen was identified. The GPB was recognized after dissection, from which the distance to the cemento‐enamel junction of the first molar and premolar was measured. Eight periodontists and twelve periodontal residents were asked to estimate the location of the GPB on the study models and the same measurements were taken. Comparisons of the estimated and true GPB position were performed. The correlation between the palatal vault height and the variability of detecting the GPB was investigated.

Results: The most frequent greater palatine foramen location was between the second and third molars (66.6%). For most cases, there was an underestimation of the location of the GPB up to 4 mm. The interexaminer variability was positively correlated with the vault height.

Conclusions: The estimated location of the GPB was commonly closer to the cemento‐enamel junction of posterior teeth. Agreement on the location of the GPB was lowered with the presence of high palatal vaults. The results of this study could assist clinicians in planning the location for harvesting SCTG on the hard palate.
Electrochemical oxidation of tetracycline antibiotics using a Ti/IrO2 anode for wastewater treatment of animal husbandry
Authors: Miyata M. , Ihara I. , Yoshid G. , Toyod K. , Umetsu K.
Published: February 2011

In animal husbandry, antibiotics are widely used to treat and prevent diseases or to promote growth. The use of antibiotics for domestic animals enables to promote safety of livestock products and enhance productivity. Tetracycline antibiotics (TCs) are one of the primarily used groups of antibiotics for cattle and swine. However, the unintentional spreading of antibiotics from animal waste to the environment may leave out drug residues, promoting resistant strains of bacteria, and will adversely affect the ecosystem and human health. To prevent the spread of veterinary antibiotics in the environment, it is required to treat residual antibiotics in livestock wastewater. In this study, we investigated the electrochemical oxidation of TCs to treat livestock wastewater. The concentrations of TCs in aqueous solutions were reduced from 100 mg/L to less than 0.6 mg/L by 6 h of electrochemical treatment using a Ti/IrO2 anode with Na2SO4 electrolyte. The concentration of oxytetracycline (OTC) in livestock wastewater was also reduced from 100 mg/L to less than 0.7 mg/L by the same treatment. Thus, the electrochemical oxidation using a Ti/IrO2 anode with Na2SO4 electrolyte was found to be effective for degradation of TCs. The results suggest that the electrochemical oxidation method is a promising treatment for TCs in livestock wastewater.
The power efficiency of the Tajo-Segura transfer and desalination
Authors: Melgarejo J. , Montano B.
Published: February 2011

The origin of the water supply system of Tajo-Segura, in 1932, goes back to when it was the unique possible solution to the shortage of hydric resources in the Confederación Hidrográfica del Segura (CHS). Its energy consumption is not homogeneous; it could be bigger or smaller, depending on the different water destination. The energy cost average of water of the Tajo-Segura water supply system is 1.21 kWh/m3. The energy consumption of desalination depends on the type of process used to desalinate the water, the time of operation and the capacity of the plant, and also on the density of water to treat. In any case, the energy consumption of the desalination ranges between 3.8 and 4.2 kWh/m3; obviously that is bigger than those obtained of the Tajo-Segura water supply system (1.21 kWh/m3).
The inter-tidal zone is the pathway of input of enterococci to a subtropical recreational marine beach
Authors: Wright M. E. , Abdelzaher A. M. , Solo-Gabriele H. M. , Elmir S. , Fleming L. E.
Published: February 2011

Efforts were made to evaluate the source of enterococci levels at a recreational beach. Four monitoring efforts were implemented which included tidal studies, hourly sampling, runoff sampling, and spatially intensive sediment sampling. Spatially intensive sediment sampling indicated that enterococci concentrations consistently decreased away from the inter-tidal zone, both seaward and landward. During dry conditions, the highest concentrations in the water were observed during high tide (71±48 CFU/100 mL) and lower concentrations were observed during low tide (4±3 CFU/100 mL). Runoff was characterised by very high levels (11,700 CFU/100 mL). Results from these monitoring efforts collectively showed that the source of enterococci to the study beach is geographically located within the inter-tidal zone. Wash-in from the inter-tidal zone through tidal action and runoff plays a major role in controlling enterococci levels within the water column. Such results are significant in identifying the source and transport mechanisms of enterococci, which can subsequently be used as part of a modelling effort aimed at predicting enterococci levels at recreational beaches.
Perceived Benefits and Psychosocial Outcomes of a Brief Existential Family Intervention for Cancer Patients/Survivors
Authors: Garlan Robert W. , Butler Lisa D. , Siegel Ernest Rosenbaum Alison , Spiegel David
Published: February 2011

This study assessed a range of benefits from participation in a brief existential intervention consisting of a semi-structured videotaped interview with cancer patients and their families designed to illuminate a life legacy for the family (the Life Tape Project [LTP]). Results indicated the majority reported intervention-specific benefits, especially in the areas of symbolic immortality (passing on personal values and philosophy), self-reflection and growth, and improved family cohesion and communication. Participants, particularly those who had perceived their cancer as a threat of death, serious injury, or threat to their physical integrity, and responded with intense fear or helplessness, also reported more general reductions in mood disturbance, improvements in aspects of well-being (including overall quality of life), satisfaction with the understanding they received, and enhanced cancer-related posttraumatic growth. In short, the LTP is a brief, inexpensive, existential intervention that can yield broad positive psychosocial changes for a majority of participants.
The energy of integral circulant graphs with prime power order
Authors: Sander J.W. , Sander T.
Published: February 2011

The energy of a graph is the sum of the moduli of the eigenvalues of its
adjacency matrix. We study the energy of integral circulant graphs, also
called gcd graphs. Such a graph can be characterized by its vertex count n
and a set D of divisors of n such that its vertex set is Zn and its edge set
is {{a,b} : a, b ? Zn; gcd(a-b, n)? D}. For an integral circulant graph
on ps vertices, where p is a prime, we derive a closed formula for its
energy in terms of n and D. Moreover, we study minimal and maximal energies
for fixed ps and varying divisor sets D.
Polymerase I and Transcript Release Factor Regulates Lipolysis via a Phosphorylation-Dependent Mechanism
Authors: Aboulaich Nabila , Chui Patricia C. , Asara John M. , Flier Jeffrey S. , Maratos-Flier Eleftheria
Published: February 2011

OBJECTIVE

Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization.

RESEARCH DESIGN AND METHODS

PTRF expression was examined in different adipose depots of mice during fasting, refeeding, and after administration of catecholamines and insulin. Involvement of PTRF during lipolysis was studied upon PTRF knockdown and overexpression and mutation of PTRF phosphorylation sites in 3T3-L1 adipocytes.

RESULTS

PTRF expression in mouse white adipose tissue (WAT) is regulated by nutritional status, increasing during fasting and decreasing to baseline after refeeding. Expression of PTRF also is hormonally regulated because treatment of mice with insulin leads to a decrease in expression, whereas isoproterenol increases expression in WAT. Manipulation of PTRF levels revealed a role of PTRF in lipolysis. Lentiviral-mediated knockdown of PTRF resulted in a marked attenuation of glycerol release in response to isoproterenol. Conversely, overexpressing PTRF enhanced isoproterenol-stimulated glycerol release. Mass-spectrometric analysis revealed that PTRF is phosphorylated at multiple sites in WAT. Mutation of serine 42, threonine 304, or serine 368 to alanine reduced isoproterenol-stimulated glycerol release in 3T3-L1 adipocytes.

CONCLUSIONS

Our study is the first direct demonstration for a novel adipose tissue–specific function of PTRF as a mediator of lipolysis and also shows that phosphorylation of PTRF is required for efficient fat mobilization.
The ENPP1 Q121 Variant Predicts Major Cardiovascular Events in High-Risk Individuals
Authors: Bacci Simonetta , Rizza Stefano , Prudente Sabrina , Spoto Belinda , Powers Christine , Facciorusso Antonio , Pacilli Antonio , Lauro Davide , Testa Alessandra , Zhang Yuan-Yuan , Di Stolfo Giuseppe , Mallamaci Francesca , Tripepi Giovanni , Xu Rui , Mangiacotti Davide , Aucella Filippo , Lauro Renato , Gervino Ernest V. , Hauser Thomas H. , Copetti Massimiliano , De Cosmo Salvatore , Pellegrini Fabio , Zoccali Carmine , Federici Massimo , Doria Alessandro , Trischitta Vincenzo
Published: February 2011

OBJECTIVE

Insulin resistance (IR) and cardiovascular disease may share a common genetic background. We investigated the role of IR-associated ENPP1 K121Q polymorphism (rs1044498) on cardiovascular disease in high-risk individuals.

RESEARCH DESIGN AND METHODS

A prospective study (average follow-up, 37 months) was conducted for major cardiovascular events (myocardial infarction [MI], stroke, cardiovascular death) from the Gargano Heart Study (GHS; n = 330 with type 2 diabetes and coronary artery disease), the Tor Vergata Atherosclerosis Study (TVAS; n = 141 who had MI), and the Cardiovascular Risk Extended Evaluation in Dialysis (CREED) database (n = 266 with end-stage renal disease). Age at MI was investigated in cross-sectional studies of 339 type 2 diabetic patients (n = 169 from Italy, n = 170 from the U.S.).

RESULTS

Incidence of cardiovascular events per 100 person--years was 4.2 in GHS, 10.8 in TVAS, and 11.7 in CREED. Hazard ratios (HRs) for KQ+QQ versus individuals carrying the K121/K121 genotype (KK) individuals were 1.47 (95% CI 0.80–2.70) in GHS, 2.31 (95% CI 1.22–4.34) in TVAS, and 1.36 (95% CI 0.88–2.10) in CREED, and 1.56 (95% CI 1.15–2.12) in the three cohorts combined. In the 395 diabetic patients, the Q121 variant predicted cardiovascular events among obese but not among nonobese individuals (HR 5.94 vs. 0.62, P = 0.003 for interaction). A similar synergism was observed in cross-sectional studies, with age at MI being 3 years younger in Q121 carriers than in KK homozygotes among obese but not among nonobese patients (P = 0.035 for interaction).

CONCLUSIONS

The ENPP1 K121Q polymorphism is an independent predictor of major cardiovascular events in high-risk individuals. In type 2 diabetes, this effect is exacerbated by obesity. Future larger studies are needed to confirm our finding.
Ethnic Differences in Diabetes Management in Patients With and Without Comorbid Medical Conditions
Authors: Alshamsan Riyadh , Majeed Azeem , Vamos Eszter Panna , Khunti Kamlesh , Curcin Vasa , Rawaf Salman , Millett Christopher
Published: February 2011

OBJECTIVE

To examine ethnic disparities in diabetes management among patients with and without comorbid medical conditions after a period of sustained investment in quality improvement in the U.K.

RESEARCH DESIGN AND METHODS

This cross-sectional study examined associations between ethnicity, comorbidity, and intermediate outcomes for mean A1C, total cholesterol, and blood pressure levels in 6,690 diabetes patients in South West London.

RESULTS

The presence of ≥2 cardiovascular comorbidities was associated with similar blood pressure control among white and South Asian patients when compared with whites without comorbidity but with worse blood pressure control among black patients, with a mean difference in systolic blood pressure of +1.5, +1.4, and +6.2 mmHg, respectively.

CONCLUSIONS

Despite major reforms to improve quality, disparities in blood pressure management have persisted in the U.K., particularly among patients with cardiovascular comorbidities. Policy makers should consider the potential impacts of quality initiatives on high-risk groups.
Prediabetes and Prehypertension in Healthy Adults Are Associated With Low Vitamin D Levels
Authors: Gupta Alok K. , Brashear Meghan M. , Johnson William D.
Published: February 2011

OBJECTIVE

To determine whether modest elevations of fasting serum glucose (FSG) and resting blood pressure (BP) in healthy adults are associated with differential serum vitamin D concentrations.

RESEARCH DESIGN AND METHODS

Disease-free adults in the National Health and Nutrition Examination Survey 2001–2006 were assessed. Prediabetes (PreDM) and prehypertension (PreHTN) were diagnosed using American Diabetes Association and Seventh Report of the Joint National Committee on Prevention, Detection, and Treatment of High Blood Pressure criteria: FSG 100–125 mg/dL and systolic BP 120–139 mmHg and/or diastolic BP 80–89 mmHg. Logistic regression was used to assess the effects of low vitamin D levels on the odds for PreDM and PreHTN in asymptomatic adults (n = 1,711).

RESULTS

The odds ratio for comorbid PreDM and PreHTN in Caucasian men (n = 898) and women (n = 813) was 2.41 (P < 0.0001) with vitamin D levels ≤76.3 versus >76.3 nmol/L after adjusting for age, sex, and BMI.

CONCLUSIONS

This study strengthens the plausibility that low serum vitamin D levels elevate the risk for early-stage diabetes (PreDM) and hypertension (PreHTN).
Perceived Impact of Diabetes Genetic Risk Testing Among Patients at High Phenotypic Risk for Type 2 Diabetes
Authors: Markowitz Sarah M. , Park Elyse R. , Delahanty Linda M. , O’Brien Kelsey E. , Grant Richard W.
Published: February 2011

OBJECTIVE

Rapid advances in diabetes genetic epidemiology may lead to a new era of “personalized medicine” based on individual genetic risk assessment. There is minimal experience to guide how best to clinically implement such testing so that results (e.g., “higher” or “lower” relative genetic risk) improve rather than reduce patient motivation for behavior change.

RESEARCH DESIGN AND METHODS

Between November 2009 and May 2010, we conducted in-depth interviews with 22 overweight participants at high phenotypic risk for type 2 diabetes to explore perceptions of diabetes genetic risk testing compared with currently available prediction using nongenetic risk factors (e.g., family history, abnormal fasting glucose, obesity). We used hypothetical scenarios to specifically investigate the impact of both “higher” and “lower” relative genetic risk results on participants’ views about diabetes prevention.

RESULTS

Many participants conferred a unique value on personal genetic risk information relative to nongenetic risk based on the perceived scientific certainty and durability of genetic results. In contrast, other participants considered their genetic risk within the overall context of their other measured risk factors. Reactions to diabetes genetic test results differed by current motivation levels. Whereas most subjects reported that “higher” risk results would motivate behavior change, subjects with lower current motivation often reported that “lower” genetic risk results would further reduce their motivation to engage in diabetes prevention behaviors.

CONCLUSIONS

To be effective, future clinical implementation of type 2 diabetes genetic risk testing should be individualized based on each patient’s risk perception and current level of motivation to prevent diabetes.
Use of a meal challenge test to estimate peak postprandial triglyceride concentrations in dogs
Authors: Elliott Kathryn F. , Rand Jacquie S. , Fleeman Linda M. , Morton John M. , Markwell Peter J.
Published: February 2011

Objective—To develop a standardized meal challenge test by assessing associations between food-withheld preprandial (ie, fasting) and postprandial triglyceride concentrations, determining the most appropriate sampling time to detect the peak concentration (highest postprandial concentration), and estimating reference intervals for fasting and postprandial concentrations in healthy dogs.

Animals—12 lean healthy mixed-breed dogs.

Procedures—Dogs were fed a dry commercially available diet (fat, 31% metabolizable energy) for 3 weeks. After food was withheld for 23 to 24 hours, plasma triglyceride concentrations were measured 1 and 0.083 hours before and 1, 2, 3, 4, 5, 6, 9, and 12 hours after feeding of a standardized challenge meal (median amount eaten, 63 kcal/kg [127 kcal/kg0.75]). Correlation and agreement between concentrations at peak and other time points were assessed by use of correlation coefficients and Bland-Altman limits of agreement. Reference intervals were calculated by use of a robust method.

Results—Fasting and peak triglyceride concentrations were not closely associated. The highest concentration among samples obtained 2, 5, and 6 hours after meal consumption had closest agreement with peak concentration. In 5 of 12 dogs, concentrations 12 hours after eating were still significantly above baseline concentration (mean of each dog's fasting concentrations).

Conclusions and Clinical Relevance—Fasting triglyceride concentration could not be used to accurately predict peak concentration. When estimating peak concentration, multiple samples should be collected 2, 5, and 6 hours after consumption of a standardized meal. Food may need to be withheld for > 12 hours when assessing fasting concentrations in healthy dogs.
Refinement and initial validation of a multidimensional composite scale for use in assessing acute postoperative pain in cats
Authors: Brondani Juliana Tabarelli , Luna Stelio Pacca Loureiro , Padovani Carlos Roberto
Published: February 2011

Objective—To refine and test construct validity and reliability of a composite pain scale for use in assessing acute postoperative pain in cats undergoing ovariohysterectomy.

Sample Population—40 cats that underwent ovariohysterectomy in a previous study.

Procedures—In a previous randomized, double-blind, placebo-controlled study, a composite pain scale was developed to assess postoperative pain in cats that received a placebo or an analgesic (tramadol, vedaprofen, or tramadol-vedaprofen combination). In the present study, the scale was refined via item analysis (distribution frequency and occurrence), a nonparametric ANOVA, and item-to-total score correlation. Construct validity was assessed via factor analysis and known-groups discrimination, and reliability was measured by assessing internal consistency.

Results—Respiratory rate and respiratory pattern were rejected after item analysis. Factor analysis resulted in 5 dimensions (F1 [psychomotor change], posture, comfort, activity, mental status, and miscellaneous behaviors; F2 [protection of wound area], reaction to palpation of the surgical wound and palpation of the abdomen and flank; F3 [physiologic variables], systolic arterial blood pressure and appetite; F4 [vocal expression of pain], vocalization; and F5 [heart rate]). Internal consistency was excellent for the overall scale and for F1, F2, and F3; very good for F4; and unacceptable for F5. Except for heart rate, the identified factors and scale total score could be used to detect differences between the analgesic and placebo groups and differences among the analgesic treatments.

Conclusions and Clinical Relevance—Results provided initial evidence of construct validity and reliability of a multidimensional composite tool for use in assessing acute postoperative pain in cats undergoing ovariohysterectomy.
Evaluation of the cyclooxygenase selectivity of robenacoxib and its effect on recovery of ischemia-injured jejunal mucosa in horses
Authors: Marshall John F , Bhatnagar Adria S. , Bowman Susan G. , Howard Christina M. , Morris Natalie N. , Skorich Dinah A. , Redding Caitlyn D. , Blikslager Anthony T.
Published: February 2011

Objective—To determine the cyclooxygenase (COX) selectivity of robenacoxib and its effect on recovery of jejunal mucosa following ischemic injury in horses.

Animals—12 healthy horses.

Procedures—Half the maximal inhibition (EC50) of robenacoxib for COX-1 and COX-2 activity was established in bloods samples from 6 horses via measurement of thromboxane B2 (TXB2) and prostaglandin E2 concentrations, respectively; COX selectivity was subsequently calculated. Six other horses were anesthetized, and ischemia was induced in the jejunum for 2 hours. Control and ischemia-injured mucosa were collected and incubated with Ringer's solution (control treatment), flunixin meglumine (2.7 × 10−5M), or robenacoxib (2.7 × 10−5M). Transepithelial electrical resistance and mannitol flux were measured over a 4-hour recovery period. Bathing solution TXB2 and prostaglandin E metabolite concentrations were measured to assess COX-1 and COX-2 function, respectively.

Results—The mean ± SD EC50 value of robenacoxib for COX-1 and COX-2 was 11.46 ± 4.46μM and 0.19 ± 0.07μM, respectively, resulting in a COX selectivity ratio of 61.01. The transepithelial electrical resistance of ischemia-injured jejunum treated with flunixin meglumine was significantly lower than that of control and robenacoxib-treated tissues. A significant increase in concentrations of prostaglandin E metabolites and TXB2 was detected in control and robenacoxib-treated tissues but not flunixin meglumine—treated tissues.

Conclusions and Clinical Relevance—Robenacoxib selectively inhibited COX-2 and allowed recovery of barrier function in ischemia-injured equine jejunal tissue in vitro.
Characterization of the temporal and spatial distribution and reproductive ratio of vesicular stomatitis outbreaks in Mexico in 2008
Authors: Arroyo Montserrat , Perez Andres M. , Rodriguez Luis L.
Published: February 2011

Objective—To characterize the temporal and spatial distribution and reproductive ratio of vesicular stomatitis (VS) outbreaks reported in Mexico in 2008.

Animals—Bovine herds in Mexico in which VS outbreaks were officially reported and confirmed from January 1 through December 31, 2008.

Procedures—The Poisson model of the space-time scan statistic was used to identify periods and geographical locations at highest risk for VS in Mexico in 2008. The herd reproductive ratio (Rh) of the epidemic was computed by use of the doubling-time method.

Results—1 significant space-time cluster of VS was detected in the state of Michoacan from September 4 through December 10, 2008. The temporal extent of the VS outbreaks and the value and pattern of decrease of the Rh were different in the endemic zone of Tabasco and Chiapas, compared with findings in the region included in the space-time cluster.

Conclusions and Clinical Relevance—The large number of VS outbreaks reported in Mexico in 2008 was associated with the spread of the disease from the endemic zone in southern Mexico to areas sporadically affected by the disease. Results suggested that implementation of a surveillance system in the endemic zone of Mexico aimed at early detection of changes in the value of Rh and space-time clustering of the disease could help predict occurrence of future VS outbreaks originating from this endemic zone. This information will help prevent VS spread into regions of Mexico and neighboring countries that are only sporadically affected by the disease.
Pharmacokinetics and antinociceptive effects of oral tramadol hydrochloride administration in Greyhounds
Authors: KuKanich Butch , Papich Mark G.
Published: February 2011

Objective—To determine the pharmacokinetics of tramadol, the active metabolite O-desmethyltrcamadol, and the metabolites N-desmethyltramadol and N,O-didesmethyltramadol after oral tramadol administration and to determine the antinociceptive effects of the drug in Greyhounds.

Animals—6 healthy 2- to 3-year-old Greyhounds (3 male and 3 female), weighing 25.5 to 41.1 kg.

Procedures—A mean dose of 9.9 mg of tramadol HCl/kg was administered PO as whole tablets. Blood samples were obtained prior to and at various points after administration to measure plasma concentrations of tramadol and its metabolites via liquid chromatography with mass spectrometry. Antinociceptive effects were determined by measurement of pain-pressure thresholds with a von Frey device.

Results—Tramadol was well tolerated, and a significant increase in pain-pressure thresholds was evident 5 and 6 hours after administration. The mean maximum plasma concentrations of tramadol, O-desmethyltramadol, N-desmethyltramadol, and N,O-didesmethyltramadol were 215.7, 5.7, 379.1, and 2372 ng/mL, respectively. The mean area-under-the-curve values for the compounds were 592, 16, 1,536, and 1,013 h·ng/mL, respectively. The terminal half-lives of the compounds were 1.1, 1.4, 2.3, and 3.6 hours, respectively. Tramadol was detected in urine 5 days, but not 7 days, after administration.

Conclusions and Clinical Relevance—Oral tramadol administration yielded antinociceptive effects in Greyhounds, but plasma concentrations of tramadol and O-desmethyltramadol were lower than expected. Compared with the approved dose (100 mg, PO) in humans, a mean dose of 9.9 mg/kg, PO resulted in similar tramadol but lower O-desmethyltramadol plasma concentrations in Greyhounds.