bluuebunny/crossref_metadata_2025 huggingface dataset:
Building Virtual 3D City Model for Smart Cities Applications: A Case Study on Campus Area of the University of Novi Sad
Authors: Jovanović Dušan , Milovanov Stevan , Ruskovski Igor , Govedarica Miro , Sladić Dubravka , Radulović Aleksandra , Pajić Vladimir
Published: July 2020

The Smart Cities data and applications need to replicate, as faithfully as possible, the state of the city and to simulate possible alternative futures. In order to do this, the modelling of the city should cover all aspects of the city that are relevant to the problems that require smart solutions. In this context, 2D and 3D spatial data play a key role, in particular 3D city models. One of the methods for collecting data that can be used for developing such 3D city models is Light Detection and Ranging (LiDAR), a technology that has provided opportunities to generate large-scale 3D city models at relatively low cost. The collected data is further processed to obtain fully developed photorealistic virtual 3D city models. The goal of this research is to develop virtual 3D city model based on airborne LiDAR surveying and to analyze its applicability toward Smart Cities applications. It this paper, we present workflow that goes from data collection by LiDAR, through extract, transform, load (ETL) transformations and data processing to developing 3D virtual city model and finally discuss its future potential usage scenarios in various fields of application such as modern ICT-based urban planning and 3D cadaster. The results are presented on the case study of campus area of the University of Novi Sad.
Daily Water Level Prediction of Zrebar Lake (Iran): A Comparison between M5P, Random Forest, Random Tree and Reduced Error Pruning Trees Algorithms
Authors: Nhu Viet-Ha , Shahabi Himan , Nohani Ebrahim , Shirzadi Ataollah , Al-Ansari Nadhir , Bahrami Sepideh , Miraki Shaghayegh , Geertsema Marten , Nguyen Hoang
Published: July 2020

Zrebar Lake is one of the largest freshwater lakes in Iran and it plays an important role in the ecosystem of the environment, while its desiccation has a negative impact on the surrounded ecosystem. Despite this, this lake provides an interesting recreation setting in terms of ecotourism. The prediction and forecasting of the water level of the lake through simple but practical methods can provide a reliable tool for future lake water resource management. In the present study, we predict the daily water level of Zrebar Lake in Iran through well-known decision tree-based algorithms, including the M5 pruned (M5P), random forest (RF), random tree (RT) and reduced error pruning tree (REPT). We used five different water input combinations to find the most effective one. For our modeling, we chose 70% of the dataset for training (from 2011 to 2015) and 30% for model evaluation (from 2015 to 2017). We evaluated the models’ performances using different quantitative (root mean square error (RMSE), mean absolute error (MAE), coefficient of determination (R2), percent bias (PBIAS) and ratio of the root mean square error to the standard deviation of measured data (RSR)) and visual frameworks (Taylor diagram and box plot). Our results showed that water level with a one-day lag time had the highest effect on the result and, by increasing the lag time, its effect on the result was decreased. This result indicated that all the developed models had a good prediction capability, but the M5P model outperformed the others, followed by RF and RT equally and then REPT. Our results showed that these algorithms can predict water level accurately only with a one-day lag time in water level as an input and they are cost-effective tools for future predictions.
Correction: Li, et al. LncRNA NEAT1 Silenced miR-133b Promotes Migration and Invasion of Breast Cancer Cells. Int. J. Mol. Sci. 2019, 20, 3616
Authors: Li Xinping , Deng Siwei , Pang Xinyao , Song Yixiao , Luo Shiyu , Jin Liang , Pan Yi
Published: July 2020

The author wishes to make the following correction to this paper [...]
Molecular Basis of Mitochondrial and Peroxisomal Division Machineries
Authors: Imoto Yuuta , Itoh Kie , Fujiki Yukio
Published: July 2020

Mitochondria and peroxisomes are ubiquitous subcellular organelles that are highly dynamic and possess a high degree of plasticity. These organelles proliferate through division of pre-existing organelles. Studies on yeast, mammalian cells, and unicellular algae have led to a surprising finding that mitochondria and peroxisomes share the components of their division machineries. At the heart of the mitochondrial and peroxisomal division machineries is a GTPase dynamin-like protein, Dnm1/Drp1, which forms a contractile ring around the neck of the dividing organelles. During division, Dnm1/Drp1 functions as a motor protein and constricts the membrane. This mechanochemical work is achieved by utilizing energy from GTP hydrolysis. Over the last two decades, studies have focused on the structure and assembly of Dnm1/Drp1 molecules around the neck. However, the regulation of GTP during the division of mitochondrion and peroxisome is not well understood. Here, we review the current understanding of Dnm1/Drp1-mediated divisions of mitochondria and peroxisomes, exploring the mechanisms of GTP regulation during the Dnm1/Drp1 function, and provide new perspectives on their potential contribution to mitochondrial and peroxisomal biogenesis.
MicroRNA-Dependent Targeting of RSU1 and the IPP Adhesion Complex Regulates the PTEN/PI3K/AKT Signaling Pathway in Breast Cancer Cell Lines
Authors: Kim Yong-Chul , Cutler Mary L.
Published: July 2020

(1) Background: The microRNA (miR)-directed control of gene expression is correlated with numerous physiological processes as well as the pathological features of tumors. The focus of this study is on the role of miRs in the regulation of RSU1 and proteins in the IPP (integrin linked kinase, PINCH and parvin) complex. Because the IPP adaptor proteins link β integrins to actin cytoskeleton, and the RSU1 signaling protein connects the complex to the activation of cJun, ATF2 and the transcription of PTEN, their reduction by miRs has the potential to alter both adhesion and survival signaling. (2) Methods: Multiple database analyses were used to identify miRs that target RSU1 and PINCH1. miR transfection validated the effects of miRs on RSU1, PINCH1 and downstream targets in breast cancer cell lines. (3) Results: The miRs targeting RSU1 mRNA include miR-182-5p, -409-3p, -130a-3p, -221-3p, -744-5p and -106b-5p. Data show that miR-182-5p and -409-3p reduce RSU1, PINCH1 and inhibit the ATF2 activation of PTEN expression. miR-221-3p and miR-130a-3p target RSU1 and PINCH1 and, conversely, RSU1 depletion increases miR-221-3p and miR-130a-3p. (4) Conclusions: miRs targeting RSU1 and PINCH1 in mammary epithelial or luminal breast cancer cell lines reduced RSU1 signaling to p38 MAP kinase and ATF2, inhibiting the expression of PTEN. miR-221-3p, known to target PTEN and cell cycle regulators, also targets RSU1 and PINCH1 in luminal breast cancer cell lines.
FcεRI Signaling in the Modulation of Allergic Response: Role of Mast Cell-Derived Exosomes
Authors: Lecce Mario , Molfetta Rosa , Milito Nadia Domenica , Santoni Angela , Paolini Rossella
Published: July 2020

Mast cells (MCs) are immune cells that act as environment resident sentinels playing a crucial role in Th2-mediated immune responses, including allergic reactions. Distinguishing features of MCs are the presence of numerous cytoplasmic granules that encapsulate a wide array of preformed bio-active molecules and the constitutive expression of the high affinity receptor of IgE (FcεRI). Upon FcεRI engagement by means of IgE and multivalent antigens, aggregated receptors trigger biochemical pathways that ultimately lead to the release of granule-stored and newly synthesized pro-inflammatory mediators. Additionally, MCs are also able to release exosomes either constitutively or upon stimulation. Exosomes are nanosized vesicles of endocytic origin endowed with important immunoregulatory properties, and represent an additional way of intercellular communication. Interestingly, exosomes generated upon FcεRI engagement contain co-stimulatory and adhesion molecules, lipid mediators, and MC-specific proteases, as well as receptor subunits together with IgE and antigens. These findings support the notion that FcεRI signaling plays an important role in influencing the composition and functions of exosomes derived by MCs depending on their activation status.
Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies
Authors: Gois Beghini Daniela , Iwao Horita Samuel , Cascabulho Cynthia Machado , Anastácio Alves Luiz , Henriques-Pons Andrea
Published: July 2020

Induced pluripotent stem (iPS) cells are laboratory-produced cells that combine the biological advantages of somatic adult and stem cells for cell-based therapy. The reprogramming of cells, such as fibroblasts, to an embryonic stem cell-like state is done by the ectopic expression of transcription factors responsible for generating embryonic stem cell properties. These primary factors are octamer-binding transcription factor 4 (Oct3/4), sex-determining region Y-box 2 (Sox2), Krüppel-like factor 4 (Klf4), and the proto-oncogene protein homolog of avian myelocytomatosis (c-Myc). The somatic cells can be easily obtained from the patient who will be subjected to cellular therapy and be reprogrammed to acquire the necessary high plasticity of embryonic stem cells. These cells have no ethical limitations involved, as in the case of embryonic stem cells, and display minimal immunological rejection risks after transplant. Currently, several clinical trials are in progress, most of them in phase I or II. Still, some inherent risks, such as chromosomal instability, insertional tumors, and teratoma formation, must be overcome to reach full clinical translation. However, with the clinical trials and extensive basic research studying the biology of these cells, a promising future for human cell-based therapies using iPS cells seems to be increasingly clear and close.
Racial Disparities-Associated COVID-19 Mortality among Minority Populations in the US
Authors: Alcendor Donald J.
Published: July 2020

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a betacoronavirus that causes the novel coronavirus disease 2019 (COVID-19), is highly transmissible and pathogenic for humans and may cause life-threatening disease and mortality, especially in individuals with underlying comorbidities. First identified in an outbreak in Wuhan, China, COVID-19 is affecting more than 185 countries and territories around the world, with more than 15,754,651 confirmed cases and more than 640,029 deaths. Since December 2019, SARS-CoV-2 transmission has become a global threat, which includes confirmed cases in all 50 states within the United States (US). As of 25 July 2020, the Johns Hopkins Whiting School of Engineering Center for Systems Science and Engineering reports more than 4,112,651 cases and 145,546 deaths. To date, health disparities are associated with COVID-19 mortality among underserved populations. Here, the author explores potential underlying reasons for reported disproportionate, increased risks of mortality among African Americans and Hispanics/Latinos with COVID-19 compared with non-Hispanic Whites. The author examines the underlying clinical implications that may predispose minority populations and the adverse clinical outcomes that may contribute to increased risk of mortality. Government and community-based strategies to safeguard minority populations at risk for increased morbidity and mortality are essential. Underserved populations living in poverty with limited access to social services across the US are more likely to have underlying medical conditions and are among the most vulnerable. Societal and cultural barriers for ethnic minorities to achieve health equity are systemic issues that may be addressed only through shifts in governmental policies, producing long-overdue, substantive changes to end health care inequities.
Effect of an Enhanced Self-Care Protocol on Lymphedema Status among People Affected by Moderate to Severe Lower-Limb Lymphedema in Bangladesh, a Cluster Randomized Controlled Trial
Authors: Douglass Janet , Mableson Hayley , Martindale Sarah , Jhara Sanya Tahmina , Karim Mohammad Jahirul , Rahman Muhammad Mujibur , Kawsar Abdullah Al , Khair Abul , Mahmood ASM Sultan , Rahman AKM Fazlur , Chowdhury Salim Mahmud , Kim Susan , Betts Hannah , Taylor Mark , Kelly-Hope Louise
Published: July 2020

Background: Lymphatic filariasis (LF) is a major cause of lymphedema, affecting over 16 million people globally. A daily, hygiene-centered self-care protocol is recommended and effective in reducing acute attacks caused by secondary infections. It may also reverse lymphedema status in early stages, but less so as lymphedema advances. Lymphatic stimulating activities such as self-massage and deep-breathing have proven beneficial for cancer-related lymphedema, but have not been tested in LF-settings. Therefore, an enhanced self-care protocol was trialed among people affected by moderate to severe LF-related lymphedema in northern Bangladesh. Methods: Cluster randomization was used to allocate participants to either standard- or enhanced-self-care groups. Lymphedema status was determined by lymphedema stage, mid-calf circumference, and mid-calf tissue compressibility. Results: There were 71 patients in each group and at 24 weeks, both groups had experienced significant improvement in lymphedema status and reduction in acute attacks. There was a significant and clinically relevant between-group difference in mid-calf tissue compressibility with the biggest change observed on legs affected by severe lymphedema in the enhanced self-care group (∆ 21.5%, −0.68 (−0.91, −0.45), p < 0.001). Conclusion: This study offers the first evidence for including lymphatic stimulating activities in recommended self-care for people affected by moderate and severe LF-related lymphedema.
Prospective Observational Study to Evaluate the Effect of Different Levels of Positive End-Expiratory Pressure on Lung Mechanics in Patients with and without Acute Respiratory Distress Syndrome
Authors: Fiedler Mascha O. , Diktanaite Dovile , Simeliunas Emilis , Pilz Maximilian , Kalenka Armin
Published: July 2020

Background: The optimal level of positive end-expiratory pressure is still under debate. There are scare data examining the association of PEEP with transpulmonary pressure (TPP), end-expiratory lung volume (EELV) and intraabdominal pressure in ventilated patients with and without ARDS. Methods: We analyzed lung mechanics in 3 patient groups: group A, patients with ARDS; group B, obese patients (body mass index (BMI) > 30 kg/m2) and group C, a control group. Three levels of PEEP (5, 10, 15 cm H2O) were used to investigate the consequences for lung mechanics. Results: Fifty patients were included, 22 in group A, 18 in group B (BMI 38 ± 2 kg/m2) and 10 in group C. At baseline, oxygenation showed no differences between the groups. Driving pressure (ΔP) and transpulmonary pressure (ΔPL) was higher in group B than in groups A and C at a PEEP of 5 cm H2O (ΔP A: 15 ± 1, B: 18 ± 1, C: 14 ± 1 cm H2O; ΔPL A: 10 ± 1, B: 13 ± 1, C: 9 ± 0 cm H2O). Peak inspiratory pressure (Pinsp) rose in all groups as PEEP increased, but the resulting driving pressure and transpulmonary pressure were reduced, whereas EELV increased. Conclusion: Measuring EELV or TPP allows a personalized approach to lung-protective ventilation.
Intraventricular Hemorrhage in Very Preterm Infants: A Comprehensive Review
Authors: Gilard Vianney , Tebani Abdellah , Bekri Soumeya , Marret Stéphane
Published: July 2020

Germinal matrix-intraventricular-intraparenchymal hemorrhage (GMH-IVH-IPH) is a major complication of very preterm births before 32 weeks of gestation (WG). Despite progress in clinical management, its incidence remains high before 27 WG. In addition, severe complications may occur such as post-hemorrhagic hydrocephalus and/or periventricular intraparenchymal hemorrhage. IVH is strongly associated with subsequent neurodevelopmental disabilities. For this review, an automated literature search and a clustering approach were applied to allow efficient filtering as well as topic clusters identification. We used a programmatic literature search for research articles related to intraventricular hemorrhage in preterms that were published between January 1990 and February 2020. Two queries ((Intraventricular hemorrhage) AND (preterm)) were used in PubMed. This search resulted in 1093 articles. The data manual curation left 368 documents that formed 12 clusters. The presentation and discussion of the clusters provide a comprehensive overview of existing data on the pathogenesis, complications, neuroprotection and biomarkers of GMH-IVH-IPH in very preterm infants. Clinicians should consider that the GMH-IVH-IPH pathogenesis is mainly due to developmental immaturity of the germinal matrix and cerebral autoregulation impairment. New multiomics investigations of intraventricular hemorrhage could foster the development of predictive biomarkers for the benefit of very preterm newborns.
Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
Authors: Serena Carolina , Queipo-Ortuño Maribel , Millan Monica , Sanchez-Alcoholado Lidia , Caro Aleidis , Espina Beatriz , Menacho Margarita , Bautista Michelle , Monfort-Ferré Diandra , Terrón-Puig Margarida , Núñez-Roa Catalina , Maymó-Masip Elsa , Rodriguez M. Mar , Tinahones Francisco J. , Espin Eloy , Martí Marc , Fernández-Veledo Sonia , Vendrell Joan
Published: July 2020

Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.
Review of the Novel Investigational Antifungal Olorofim
Authors: Wiederhold Nathan P.
Published: July 2020

The incidence of invasive fungal infections caused by molds and endemic fungi is increasing. There is also concern regarding increased rates of reduced susceptibility or frank resistance among Aspergillus and Coccidioides species, while Scedosporium species, Lomentospora prolificans, and Fusarium species are inherently less susceptible or intrinsically resistant to clinically available antifungals. Olorofim (formerly F901318) is the first member of the orotomide class of antifungals to be evaluated clinically for the treatment of invasive mold infections. This agent inhibits dihydroorotate dehydrogenase, a key enzyme in the biosynthesis of pyrimidines. Olorofim has activity against many molds and thermally dimorphic fungi, including species that are resistant to azoles and amphotericin B, but lacks activity against yeasts and the Mucorales. It is currently being developed for both oral and intravenous administration. Although published clinical outcome data have been limited to case reports to date, the results against invasive and refractory infections are promising. This review describes the mechanism of action of olorofim, its in vitro spectrum of activity, and what is currently known about its pharmacokinetic profile and clinical efficacy.
Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin
Authors: Santos Carla , Santos da Silva Blenda Naara , Amorim Ferreira e Ferreira Ana Francisca Tibúrcia , Santos Cledir , Lima Nelson , Silva Bentes Jânia Lília da
Published: July 2020

Guarana plant is a native of the Amazon region. Due to its high amount of caffeine and tannins, the seed has medicinal and stimulating properties. The guarana industry has grown exponentially in recent years; however, little information is available about associated mycobiota, particularly endophytic fungi. The present study aimed to compare the distribution and diversity of endophytic fungi associated with the leaves and seeds of anthracnose-resistant and susceptible guarana plants produced in Maués and Manaus, Amazonas State, Brazil. A total of 7514 endophytic fungi were isolated on Potato Dextrose Agar, Sabouraud and Czapek media, and grouped into 77 morphological groups. Overall, fungal communities in guarana leaves and seeds were mainly composed by Colletotrichum and Fusarium genera, but also by Chondrostereum, Clonostachys, Curvularia, Hypomontagnella, Lentinus, Neopestalotiopsis, Nigrospora, Peroneutypa, Phyllosticta, Simplicillium and Tinctoporellus. Obtained results indicate that some members of Colletotrichum and Fusarium genera may have experienced dysbiosis during the guarana domestication process, suggesting that some individuals may behave as latent pathogens. The susceptible guarana genotype cultivated in Manaus presented higher fungal diversity. The relative abundance of taxa and diversity among samples suggests that communities are structured by genotype and geographic location. This is the first report of mycobiota in both guarana leaves and seeds.
The Design and Calibration of Instrumented Particles for Assessing Water Infrastructure Hazards
Authors: Al-Obaidi Khaldoon , Xu Yi , Valyrakis Manousos
Published: July 2020

The highly dynamical entrainment and transport processes of solids due to geophysical flows is a major challenge studied by water infrastructure engineers and geoscientists alike. A miniaturised instrumented particle that can provide a direct, non-intrusive, low-cost and accessible method compared to traditional approaches for the assessment of coarse sediment particle entrainment is developed, calibrated and tested. The instrumented particle presented here is fitted with inertial microelectromechanical sensors (MEMSs), such as a triaxial accelerometer, a magnetometer and angular displacement sensors, which enable the recording of the particle’s three-dimensional displacement. The sensor logs nine-axis data at a configurable rate of 200–1000 Hz and has a standard mode of deployment time of at least one hour. The data can be obtained and safely stored in an internal memory unit and are downloadable to a PC in an accessible manner and in a usable human-readable state. A plethora of improved design specifications have been implemented herein, including increased frequency, range and resolution of acceleration and gyroscopic sensing. Improvements in terms of power consumption, in comparison to previous designs, ensure longer periods of data logging. The embedded sensors are calibrated using simple physical motions to validate their operation. The uncertainties in the experiments and the sensors’ readings are quantified and an appropriate filter is used for inertial sensor fusion and noise reduction. The instrumented particle is tested under well-controlled lab conditions, where the beginning of the destabilisation of a bed surface in an open channel flow, is showcased. This is demonstrative of the potential that specifically designed and appropriately calibrated instrumented particles have in assessing the initiation and occurrence of water infrastructure hazards.
Rururban Partnerships: Urban Accessibility and Its Influence on the Stabilization of the Population in Rural Territories (Extremadura, Spain)
Authors: Gurría Gascón José Luis , Nieto Masot Ana
Published: July 2020

The process of population concentration in cities is a worldwide phenomenon—not yet finished—which has led to a widespread rural exodus and abandonment of rural areas. In Spain it occurred very abruptly from 1960, leaving numerous population centers abandoned in the northern half of the country. It is the so-called “empty Spain”. This problem has recently transcended from the local to the European level and has become part of all political agendas such as “the fight against the demographic challenge”, which the European Commission will finance in the next programming period 2021–2027. However, retaining the population in rural areas is a very complex problem that is difficult to solve. The aim of this article is to show that a polycentric system of towns, well distributed throughout the territory—as happens in Extremadura—has sufficient capacity to stabilize the population in the rural environment and is a viable and global alternative to the demographic challenge through the rururban partnerships and the integrated territorial investments. This article studies, as an empirical reality and demonstration effect, the autonomous community of Extremadura, an inland region bordering Portugal, in the southwest of the Iberian Peninsula, which has no abandoned nucleus and still maintains 50% of its population in rural areas, compared to a national average of less than 20%.
Study on the Sulfuration Mechanism of Concrete: Microstructure and Product Analysis
Authors: Niu Ditao , Lv Yao , Liu Xiguang , Chen Lei , Chen Guoxin , Zhang Binqiang
Published: July 2020

This paper presents an experimental investigation of the sulfuration mechanism of concrete. The microstructure, mineral phase composition, substance content, and pH of the concrete were determined using scanning electron microscopy, X-ray diffraction, comprehensive thermal analysis, and pore-solution pH test. It was observed that light-grey spots appeared on the surface of the specimen, and a large amount of powdery precipitated substances appeared. At the initial stage of sulfuration reaction, the formation of ettringite blocked the concrete pores and densified its cracks and voids. Subsequently, ettringite reacted with H+ to form gypsum, and the continuous increase in gypsum in the pores increased the number of cracks and broadened their width. Gypsum was the final product of the sulfuration reaction, and the mass percentage of gypsum in the powdery precipitated substances at different water–cement ratios was more than 50%. When the water–cement ratios was 0.37, 0.47, and 0.57, the highest Ca(OH)2 content was found for the lowest water–cement ratio. As the water–cement ratios increased, the amount of powdery precipitated substances decreased and the CaCO3 content and pH increased.
Surface Modeling from 2D Contours with an Application to Craniofacial Fracture Construction
Authors: Majeed Abdul , Abbas Muhammad , Miura Kenjiro T. , Kamran Mohsin , Nazir Tahir
Published: July 2020

Treating trauma to the cranio-maxillofacial region is a great challenge and requires expert clinical skills and sophisticated radiological imaging. The aim of reconstruction of the facial fractures is to rehabilitate the patient both functionally and aesthetically. Bio-modeling is an important tool for constructing surfaces using 2D cross sections. The aim of this manuscript was to show 3D construction using 2D CT scan contours. The fractured part of the cranial vault were constructed using a Ball curve with two shape parameters, later the 2D contours were flipped into 3D with an equidistant z component. The surface created was represented by a bi-cubic rational Ball surface with C2 continuity. At the end of this article, we present two real cases, in which we had constructed the frontal and parietal bone fractures using a bi-cubic rational Ball surface. The proposed method was validated by constructing the non-fractured part.
Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration
Authors: Sapoń Karolina , Maziarz Dominika , Janas Teresa , Sikorski Aleksander F. , Janas Tadeusz
Published: July 2020

We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-forming lipids (cholesterol, sphingolipids, and saturated phospholipids) and in GM1 ganglioside. CTB binds specifically and with high affinity to exosomal GM1 ganglioside residing in rafts only, and it has long been the probe of choice for membrane rafts. The CTB-gel filtration assay allows for detection of as little as 3 × 108 isolated exosomes/mL in a standard fluorometer, which has a sensitivity comparable to other methods using advanced instrumentation. The linear quantitation range for CTB-gel filtration assay extends over one order of magnitude in exosome concentration. Using 80 nM fluorescence-labeled CTB, we quantitated 3 × 108 to 6 × 109 exosomes/mL. The assay ranges exhibited linear fluorescence increases versus exosome concentration (r2 = 0.987). The assay was verified for exosomal liposomes. The assay is easy to use, rapid, and does not require any expensive or sophisticated instrumentation.
Effects of Maresin 1 (MaR1) on Colonic Inflammation and Gut Dysbiosis in Diet-Induced Obese Mice
Authors: León Irene C. , Quesada-Vázquez Sergio , Sáinz Neira , Guruceaga Elizabeth , Escoté Xavier , Moreno-Aliaga María Jesús
Published: July 2020

The aim of this study was to characterize the effects of Maresin 1 (MaR1), a DHA-derived pro-resolving lipid mediator, on obesity-related colonic inflammation and gut dysbiosis in diet-induced obese (DIO) mice. In colonic mucosa of DIO mice, the MaR1 treatment decreased the expression of inflammatory genes, such as Tnf-α and Il-1β. As expected, the DIO mice exhibited significant changes in gut microbiota composition at the phylum, genus, and species levels, with a trend to a higher Firmicutes/Bacteroidetes ratio. Deferribacteres and Synergistetes also increased in the DIO animals. In contrast, these animals exhibited a significant decrease in the content of Cyanobacteria and Actinobacteria. Treatment with MaR1 was not able to reverse the dysbiosis caused by obesity on the most abundant phyla. However, the MaR1 treatment increased the content of P. xylanivorans, which have been considered to be a promising probiotic with healthy effects on gut inflammation. Finally, a positive association was found between the Deferribacteres and Il-1β expression, suggesting that the increase in Deferribacteres observed in obesity could contribute to the overexpression of inflammatory cytokines in the colonic mucosa. In conclusion, MaR1 administration ameliorates the inflammatory state in the colonic mucosa and partially compensates changes on gut microbiota caused by obesity.
Evaluating the Antiparasitic Activity of Novel BPZ Derivatives Against Toxoplasma gondii
Authors: Hatton Olivia , Stitzlein Lea , Dudley Richard W. , Charvat Robert A.
Published: July 2020

Prevalence studies revealed that one-third of the human population is chronically infected with Toxoplasma gondii. Presently, such infections are without medical treatment that effectively eradicates the parasite once it is in its latent form. Moreover, the therapeutics used to treat acute infections are poorly tolerated by patients and also cause the parasite to convert into long-lasting tissue cysts. Hence, there is a dire need for compounds with antiparasitic activity against all forms of T. gondii. This study examines the antiparasitic capacity of nine novel bisphenol Z (BPZ) derivatives to determine whether they possessed any activity that prevented T. gondii replication. To begin assessing the efficacy of the novel derivatives, parasites were treated with increasing concentrations of the compounds, then doubling assays and MitoTracker staining were performed. Three of the nine compounds demonstrated strong inhibitory activity, i.e., parasite replication significantly decreased with higher concentrations. Additionally, many of the treated parasites exhibited decreases in fluorescent signaling and disruption of mitochondrial morphology. These findings suggest that bisphenol Z compounds disrupt mitochondrial function to inhibit parasite replication and may provide a foundation for the development of new and effective treatment modalities against T. gondii.
Collecting Agent–Mineral Interactions in the Reverse Flotation of Iron Ore: A Brief Review
Authors: Fan Guixia , Wang Liguang , Cao Yijun , Li Chao
Published: July 2020

Froth flotation has been widely used in upgrading iron ores. Iron ore flotation can be performed in two technical routes: direct flotation of iron oxides and reverse flotation of gangue minerals with depression of iron oxides. Nowadays, reverse flotation is the most commonly used route in iron ore flotation. This review is focused on the reverse flotation of iron ores, consisting of reverse cationic flotation and reverse anionic flotation. It covers different types of collecting agents used in reverse iron ore flotation, the surface characteristics of minerals commonly present in iron ores (e.g., iron oxides, quartz, alumina-bearing minerals, phosphorus-bearing minerals, iron-bearing carbonates, and iron-bearing silicates), and the adsorption mechanisms of the collecting agents at the mineral surface. The implications of collecting agent–mineral interactions for improving iron ore flotation are discussed.
Synthesis of a Rationally Designed Multi-Component Photocatalyst Pt:SiO2:TiO2(P25) with Improved Activity for Dye Degradation by Atomic Layer Deposition
Authors: Benz Dominik , Van Bui Hao , Hintzen Hubertus T. , Kreutzer Michiel T. , van Ommen J. Ruud
Published: July 2020

Photocatalysts for water purification typically lack efficiency for practical applications. Here we present a multi-component (Pt:SiO2:TiO2(P25)) material that was designed using knowledge of reaction mechanisms of mono-modified catalysts (SiO2:TiO2, and Pt:TiO2) combined with the potential of atomic layer deposition (ALD). The deposition of ultrathin SiO2 layers on TiO2 nanoparticles, applying ALD in a fluidized bed reactor, demonstrated in earlier studies their beneficial effects for the photocatalytic degradation of organic pollutants due to more acidic surface Si–OH groups which benefit the generation of hydroxyl radicals. Furthermore, our investigation on the role of Pt on TiO2(P25), as an improved photocatalyst, demonstrated that suppression of charge recombination by oxygen adsorbed on the Pt particles, reacting with the separated electrons to superoxide radicals, acts as an important factor for the catalytic improvement. Combining both materials into the resulting Pt:SiO2:TiO2(P25) nanopowder exceeded the dye degradation performance of both the individual SiO2:TiO2(P25) (1.5 fold) and Pt:TiO2(P25) (4-fold) catalysts by 6-fold as compared to TiO2(P25). This approach thus shows that by understanding the individual materials’ behavior and using ALD as an appropriate deposition technique enabling control on the nano-scale, new materials can be designed and developed, further improving the photocatalytic activity. Our research demonstrates that ALD is an attractive technology to synthesize multicomponent catalysts in a precise and scalable way.
Alternating Gyroid Network Structure in an ABC Miktoarm Terpolymer Comprised of Polystyrene and Two Polydienes
Authors: Moschovas Dimitrios , Manesi Gkreti-Maria , Karydis-Messinis Andreas , Zapsas George , Ntetsikas Konstantinos , Zafeiropoulos Nikolaos E. , Piryazev Alexey A. , Thomas Edwin L. , Hadjichristidis Nikos , Ivanov Dimitri A. , Avgeropoulos Apostolos
Published: July 2020

The synthesis, molecular and morphological characterization of a 3-miktoarm star terpolymer of polystyrene (PS, M¯n = 61.0 kg/mol), polybutadiene (PB, M¯n = 38.2 kg/mol) and polyisoprene (PI, M¯n = 29.2 kg/mol), corresponding to volume fractions (φ) of 0.46, 0.31 and 0.23 respectively, was studied. The major difference of the present material from previous ABC miktoarm stars (which is a star architecture bearing three different segments, all connected to a single junction point) with the same block components is the high 3,4-microstructure (55%) of the PI chains. The interaction parameter and the degree of polymerization of the two polydienes is sufficiently positive to create a three-phase microdomain structure as evidenced by differential scanning calorimetry and transmission electron microscopy (TEM). These results in combination with small-angle X-ray scattering (SAXS) and birefringence experiments suggest a cubic tricontinuous network structure, based on the I4132 space group never reported previously for such an architecture.
CO2 Conversion into N-Doped Porous Carbon-Encapsulated NiO/Ni Composite Nanomaterials as Outstanding Anode Material of Li Battery
Authors: Li Yayong , Xu Chunxiao , Liu Kaiyuan , Chen Pengwan , Gao Xin
Published: July 2020

N-doped porous carbon encapsulated NiO/Ni composite nanomaterials (N-doped NiO/Ni@C) was successfully obtained by a one-step solution combustion method. This study demonstrates a one-step combustion method to synthesize n-doped porous carbon encapsulated NiO/Ni composite nanomaterials, using carbon dioxide as the carbon source, nickel nitrate as the nickel source, and hydrazine hydrate as the reaction solution. Spherical NiO nanoparticles with a particle size of 20 nm were uniformly distributed in the carbon matrix. The load of NiO/Ni can be controlled by the amount of nickel nitrate. The range of carbon content of recovered samples is 69–87 at%. The content of incorporated nitrogen for recovered samples is 1.94 at%. As the anode of lithium ion battery, the composite material exhibits high capacity, excellent multiplier performance and stable circulation performance. N-doped NiO/Ni@C-2 was applied to lithium ion batteries, and its reversible capacity maximum is 980 mAh g−1 after 100 cycles at the current density of 0.1 A g−1. Its excellent electrochemical properties imply its high potential application for high-performance lithium-ion battery anode materials.
A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids
Authors: Wang Ziran , Hao Zhuang , Yu Shifeng , Huang Cong , Pan Yunlu , Zhao Xuezeng
Published: July 2020

A wearable and deformable graphene-based field-effect transistor biosensor is presented that uses aptamer-modified graphene as the conducting channel, which is capable of the sensitive, consistent and time-resolved detection of cytokines in human biofluids. Based on an ultrathin substrate, the biosensor offers a high level of mechanical durability and consistent sensing responses, while conforming to non-planar surfaces such as the human body and withstanding large deformations (e.g., bending and stretching). Moreover, a nonionic surfactant is employed to minimize the nonspecific adsorption of the biosensor, hence enabling cytokine detection (TNF-α and IFN-γ, significant inflammatory cytokines, are used as representatives) in artificial tears (used as a biofluid representative). The experimental results demonstrate that the biosensor very consistently and sensitively detects TNF-α and IFN-γ, with limits of detection down to 2.75 and 2.89 pM, respectively. The biosensor, which undergoes large deformations, can thus potentially provide a consistent and sensitive detection of cytokines in the human body.
Euglena Gracilis and β-Glucan Paramylon Induce Ca2+ Signaling in Intestinal Tract Epithelial, Immune, and Neural Cells
Authors: Yasuda Kosuke , Nakashima Ayaka , Murata Ako , Suzuki Kengo , Adachi Takahiro
Published: July 2020

The intestinal tract contains over half of all immune cells and peripheral nerves and manages the beneficial interactions between food compounds and the host. Paramylon is a β-1,3-glucan storage polysaccharide from Euglena gracilis (Euglena) that exerts immunostimulatory activities by affecting cytokine production. This study investigated the signaling mechanisms that regulate the beneficial interactions between food compounds and the intestinal tract using cell type-specific calcium (Ca2+) imaging in vivo and in vitro. We successfully visualized Euglena- and paramylon-mediated Ca2+ signaling in vivo in intestinal epithelial cells from mice ubiquitously expressing the Yellow Cameleon 3.60 (YC3.60) Ca2+ biosensor. Moreover, in vivo Ca2+ imaging demonstrated that the intraperitoneal injection of both Euglena and paramylon stimulated dendritic cells (DCs) in Peyer’s patches, indicating that paramylon is an active component of Euglena that affects the immune system. In addition, in vitro Ca2+ imaging in dorsal root ganglia indicated that Euglena, but not paramylon, triggers Ca2+ signaling in the sensory nervous system innervating the intestine. Thus, this study is the first to successfully visualize the direct effect of β-1,3-glucan on DCs in vivo and will help elucidate the mechanisms via which Euglena and paramylon exert various effects in the intestinal tract.
Detection of Epstein-Barr Virus DNA in Gastric Biopsies of Pediatric Patients with Dyspepsia
Authors: Morales-Sánchez Abigail , Torres Javier , Cardenas-Mondragón María G. , Romo-González Carolina , Camorlinga-Ponce Margarita , Flores-Luna Lourdes , Fuentes-Pananá Ezequiel M.
Published: July 2020

In this study, we assessed the presence of Epstein-Barr virus (EBV) in gastric samples derived from pediatric patients with dyspeptic symptoms, aiming to understand whether EBV participates in the development of early gastric lesions influencing chronic inflammation, in conjunction with the Helicobacter pylori (Hp) bacterium. We analyzed EBV load in 236 gastric biopsies derived from 186 pediatric patients with chronic dyspepsia and compared it with EBV serology, Hp load and serology, and with immune cell infiltration. We found that 7.5% of patients were positive for EBV load, ranging from 240 to 29,685 genomic copies/μg of DNA. Hp genomic sequences were found in 24.7% of patients. EBV positive samples did not correlate with Hp status and were characterized by absent to moderate immune cell infiltration. To our knowledge, this is the first study addressing EBV load in the stomach in a large cohort of pediatric patients with dyspeptic symptoms, providing evidence of EBV localization in the gastric mucosa in early inflammatory lesions. The lack of correlation between EBV and both Hp infection and inflammation is perhaps explained by independent pathogenic mechanisms or because of the randomness of the gastritis sampling. This is also supported by a moderate association between EBV load and serology.
A Sensitive, One-Way Sequential Sieving Method to Isolate Helminths’ Eggs and Protozoal Oocysts from Lettuce for Genetic Identification
Authors: Guggisberg Annina R. , Alvarez Rojas Cristian A. , Kronenberg Philipp A. , Miranda Nadia , Deplazes Peter
Published: July 2020

Different helminths and protozoa are transmitted to humans by oral uptake of environmentally resistant parasite stages after hand-to-mouth contact or by contaminated food and water. The aim of this study was to develop and validate a method for the simultaneous detection of parasite stages from fresh produce (lettuce) by a one-way isolation test kit followed by genetic identification (PCR, sequencing). Three sentinel zoonotic agents (eggs of Toxocara canis, Echinococcus multilocularis and oocysts of Toxoplasma gondii) were used to investigate the practicability and sensitivity of the method. The detection limits (100% positive results) in the recovery experiments were four Toxocara eggs, two E. multilocularis eggs and 18 T. gondii oocysts (in 4/5 replicates). In a field study, helminth DNA was detected in 14 of 157 lettuce samples including Hydatigera taeniaeformis (Syn. Taenia taeniaeformis) (four samples), T. polyacantha (three), T. martis (one), E. multilocularis (two) and Toxocara cati (four). Toxoplasma gondii was detected in six of 100 samples. In vivo testing in mice resulted in metacestode growth in all animals injected with 40–60 E. multilocularis eggs, while infection rates were 20–40% with 2–20 eggs. The developed diagnostic strategy is highly sensitive for the isolation and genetic characterisation of a broad range of parasite stages from lettuce, whereas the sensitivity of the viability tests needs further improvement.
Potentially Inappropriate Drug Prescribing in French Nursing Home Residents: An Observational Study
Authors: Qassemi Soraya , Pagès Arnaud , Rouch Laure , Bismuth Serge , Stillmunkes André , Lapeyre-Mestre Maryse , McCambridge Cécile , Cool Charlène , Cestac Philippe
Published: July 2020

Purpose: To identify the prevalence of potentially inappropriate drug prescription in a sample of nursing home residents in France, combining explicit criteria and implicit approach and to involve pharmacists in the multi-professional process of therapeutic optimization. Methods: A cross-sectional, observational, multicenter study was conducted during a five-month period in a sample of French nursing homes. Information on drug prescription, diseases, and socio-demographic characteristics of nursing home residents was collected. For each prescription, identification of potentially inappropriate drug prescription was done, based on explicit and implicit criteria. Results: Nursing home residents were administered an average of 8.1 (SD 3.2, range 0–20) drugs per day. Nearly 87% (n = 237) of the residents had polypharmacy with five or more drugs prescribed per day. Among the 274 nursing home residents recruited from five nursing homes, 212 (77.4%) had at least one potentially inappropriate drug prescription. According to the Laroche list, 84 residents (30.7%) had at least one drug with an unfavorable benefit–harm balance. An overdosing was found for 20.1% (n = 55) of the residents. Nearly 30% (n = 82) of the residents had a drug prescribed without valid medical indication. Conclusions: This study shows that potentially inappropriate drug prescriptions are highly prevalent among nursing home residents, nevertheless pharmacists can take part in drug utilization review in collaboration with the nursing home staff.
Digital Absolute Gene Expression Analysis of Essential Starch-Related Genes in a Radiation Developed Amaranthus cruentus L. Variety in Comparison with Real-Time PCR
Authors: Lancíková Veronika , Hricová Andrea
Published: July 2020

We investigated the expression pattern of four major starch genes at different seed developmental stages in the radiation-bred amaranth variety “Pribina” (Amaranthus cruentus L.) and corresponding control genotype “Ficha” (Amaranthus cruentus L.). Two platforms were used and compared for the gene expression analysis of GBSSI, SSSI, SBE, and DBE amaranth genes, including a standard quantitative real-time PCR (qPCR) technique and relatively novel droplet digital PCR (ddPCR) assay. In our conditions, both methods showed great accuracy and revealed higher expression of the investigated genes in the mutant variety than in the control genotype. Here we report for the first time, a ddPCR gene expression assay for the cultivated grain amaranth, as the most important group of the species in the genus Amaranthus.