bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics of heat and mass transfer: Ree-Eyring nanofluid flow over a Riga plate with bioconvention and thermal radiation
Authors: Loganathan K. , Alessa Nazek , Jain Reema , Ali Farhan , Zaib Aurang
Published: October 2022

Following improvements in devices used in biomedical engineering, cancer treatments, and thermal extrusion systems, this report explores the dynamics of Ree-Eyring nanofluid when subject to free convection, bioconvection, heat source, and thermal radiation over a convection-heated Riga plate. Bioconvection is assessed in light of the movement of the motile microorganisms that stabilize the dispersion of nanoparticles in the fluid. The impact of thermophoresis and Brownian motion, critical in the flow of heat and mass is also considered, together with the convective boundary condition. In many manufacturing sectors, non-Newtonian nanofluid flow is a crucial cooling component. Based on these factors, partial differential equations—the governing equations that model the transportation phenomena—are converted into nonlinear ordinary differential equations using the relevant relations. Finally, the nonlinear differential equations are solved using the homotopy analysis method (HAM), and the solutions are displayed in graphs representing distinct fluid flow parameters. It is conclusively found that the skin friction coefficient increases as the mixed convection parameter value rises, while the opposite effect is seen as the bioconvection Rayleigh number grows.
Dry immersion induced acute low back pain and its relationship with trunk myofascial viscoelastic changes
Authors: Plehuna Anastasija , Green David Andrew , Amirova Liubov E. , Tomilovskaya Elena S. , Rukavishnikov Ilya V. , Kozlovskaya Inessa B.
Published: October 2022

Microgravity induces spinal elongation and Low Back Pain (LBP) but the pathophysiology is unknown. Changes in paraspinal muscle viscoelastic properties may play a role. Dry Immersion (DI) is a ground-based microgravity analogue that induces changes in m. erector spinae superficial myofascial tissue tone within 2 h. This study sought to determine whether bilateral m. erector spinae tone, creep, and stiffness persist beyond 2 h; and if such changes correlate with DI-induced spinal elongation and/or LBP.

Ten healthy males lay in the DI bath at the Institute of Biomedical Problems (Moscow, Russia) for 6 h. Bilateral lumbar (L1, L4) and thoracic (T11, T9) trunk myofascial tone, stiffness and creep (MyotonPRO), and subjective LBP (0-10 NRS) were recorded before DI, after 1h, 6 h of DI, and 30min post. The non-standing spinal length was evaluated on the bath lifting platform using a bespoke stadiometer before and following DI.

DI significantly modulated m. erector spinae viscoelastic properties at L4, L1, T11, and T9 with no effect of laterality. Bilateral tissue tone was significantly reduced after 1 and 6 h DI at L4, L1, T11, and T9 to a similar extent. Stiffness was also reduced by DI at 1 h but partially recovered at 6 h for L4, L1, and T11. Creep was increased by DI at 1 h, with partial recovery at 6 h, although only T11 was significant. All properties returned to baseline 30 min following DI. Significant spinal elongation (1.17 ± 0.20 cm) with mild (at 1 h) to moderate (at 6 h) LBP was induced, mainly in the upper lumbar and lower thoracic regions. Spinal length increases positively correlated (Rho = 0.847, p = 0.024) with middle thoracic (T9) tone reduction, but with no other stiffness or creep changes. Spinal length positively correlated (Rho = 0.557, p = 0.039) with Max LBP; LBP failed to correlate with any m. erector spinae measured parameters.

The DI-induced bilateral m. erector spinae tone, creep, and stiffness changes persist beyond 2 h. Evidence of spinal elongation and LBP allows suggesting that the trunk myofascial tissue changes could play a role in LBP pathogenesis observed in real and simulated microgravity. Further study is warranted with longer duration DI, assessment of IVD geometry, and vertebral column stability.
ZmLBD2 a maize (Zea mays L.) lateral organ boundaries domain (LBD) transcription factor enhances drought tolerance in transgenic Arabidopsis thaliana
Authors: Jiao Peng , Wei Xiaotong , Jiang Zhenzhong , Liu Siyan , Guan Shuyan , Ma Yiyong
Published: October 2022

Maize (Zea mays L.) is an annual gramineous herb and is among the world’s most important crop species. Drought is the main factor contributing to maize yield reduction. The lateral organ boundaries domain (LBD) proteins belong to a class of higher-plant-specific transcription factors. LBD proteins usually include the highly conserved lateral organ boundaries (LOB) domains that play essential roles in plant growth and response to biotic stresses. However, few studies have addressed the biological functions of LBD genes associated with maize response to drought. Here we cloned the ZmLBD2 gene from maize and described its role in combating drought. Investigating ZmLBD2 subcellular localization, we show that it localizes to the cell nucleus and can specifically bind with inverted repeats of “GCGGCG”. Under drought stress, Arabidopsis thaliana overexpressing ZmLBD2 performed better than the wild-type plants in terms of seed germination rates, root length, relative water content, fresh weight, chlorophyll content, proline content, and antioxidant enzyme content. Arabidopsis overexpressing ZmLBD2 contained less MDA, H2O2, and O2− than the wild-type plants. Our protein-protein interaction results indicate an interaction between the ZmLBD2 and ZmIAA5 genes. In conclusion, the ZmLBD2 gene positively regulates H2O2 homeostasis in plants, strengthening drought resistance.
Different adaptive patterns of wheat with different drought tolerance under drought stresses and rehydration revealed by integrated metabolomic and transcriptomic analysis
Authors: Lv Liangjie , Chen Xiyong , Li Hui , Huang Jinan , Liu Yuping , Zhao Aiju
Published: October 2022

Wheat as a staple food crop is enduring ever-frequent intermittent and changing drought with the climate change. It is of great significance to highlight the adaptive approaches under such variable conditions at multiple levels to provide a comprehensive understanding of drought tolerance and facilitate the genetic breeding of wheat. Therefore, three wheat lines with different drought tolerance (drought-tolerant mutant Mu > common wheat CK > drought susceptible mutant mu) were analyzed under moderate and severe drought stresses as well as rehydration. Samples were subjected to transcriptomic and metabolomic profiling in combination with physiological and biochemical determination. The moderate drought stress rendered 198 and 115 differentially expressed metabolites (DEMs) in CK and Mu, respectively. The severe drought stress rendered 166, 151 and 137 DEMs in CK, Mu and mu, respectively. The rehydration rendered 150 and 127 DEMs in CK and Mu. 12,557 and 10,402 differentially expressed genes (DEGs) were identified for CK and Mu under moderate drought stress, respectively. 9,893, 7,924, and 9,387 DEGs were identified for CK, Mu, and mu under severe drought stress, respectively. 13,874 and 14,839 were identified in CK and Mu under rehydration, respectively. Metabolomics results showed that amino acid was the most differentially expressed metabolites, followed by phenolic acids. Flavonoids played an important role in drought tolerance. Most enriched pathways under drought included biosynthesis of secondary metabolites, metabolic pathways and photosynthesis. Metabolites and genes involved in osmotic regulation, antioxidase activities, and ABA signaling were more enriched in Mu than in CK and mu. Various drought-responsive genes and metabolites in Mu showed different trends with those in CK and mu. Increased amino acids biosynthetic capability and ROS scavenging ability resulted from higher antioxidase activities and increased flavonoids may be the mechanisms underlying the drought tolerance characteristic of Mu. Recovery from reversible ROS damage and rapid amino acid biosynthesis may contribute to the rapid recovery of Mu. The present study provides new insights for mechanisms of wheat under complex drought conditions.
Assessment of PGP traits of Bacillus cereus NDRMN001 and its influence on Cajanus cajan (L.) Millsp. phytoremediation potential on metal-polluted soil under controlled conditions
Authors: Narayanan Mathiyazhagan , Pugazhendhi Arivalagan , Ma Ying
Published: October 2022

The current study looked at the plant growth-promoting (PGP) traits of the pre-isolated and metal-tolerantBacillus cereusNDRMN001 as well as their stimulatory effect on the physiology, biomolecule content, and phytoremediation potential ofCajanus cajan(L.) Millsp. on metal-polluted soil. The bauxite mine, which is surrounded by farmland (1 km away), has been severely polluted by metals such as Cd (31.24 ± 1.68), Zn (769.57 ± 3.46), Pb (326.85 ± 3.43), Mn (2519.6 ± 5.71), and Cr (302.34 ± 1.62 mg kg−1) that exceeded Indian standards. The metal-tolerantB. cereusNDRMN001 had excellent PGP activities such as synthesis of hydrogen cyanide (HCN), siderophore, indole acetic acid (IAA), N2fixation, and P solubilization. Furthermore, the optimal growth conditions (temperature of 30°C, pH 6.5, 6% glucose, 9% tryptophan, and 1.5% tricalcium phosphate) for effective synthesis and expression of PGP traits inB. cereusNDRMN001 were determined. Such metal-tolerantB. cereusNDRMN001 traits can significantly reduce metals in polluted soil, and their PGP traits significantly improve plant growth in polluted soil. Hence, this strain (B. cereusNDRMN001) significantly improved the growth and phytoremediation potential ofC. cajan(L.) Millsp on metal-polluted soil without [study I: 2 kg of sieved and autoclaved metal-polluted soil seeded with bacterium-freeC. cajan(L.) Millsp. seeds] and with [study II: 2 kg of sieved and autoclaved metal-polluted soil seeded withB. cereusNDRMN001-coatedC. cajan(L.) Millsp. seeds]B. cereusNDRMN001 amalgamation. Fertile soil was used as control. The physiological parameters, biomolecule contents, and the phytoremediation (Cr: 7.74, Cd: 12.15, Zn: 16.72, Pb: 11.47, and Mn: 14.52 mg g−1) potential ofC. cajan(L.) Millsp. were significantly effective in study II due to the metal-solubilizing and PGP traits ofB. cereusNDRMN001. These results conclude that the test bacteriaB. cereusNDRMN001 considerably improved the phytoremediation competence ofC. cajan(L.) Millsp. on metal-polluted soil in a greenhouse study.
Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar
Authors: Cerny Martin , Berka Miroslav , Dvořák Miloň , Milenković Ivan , Saiz-Fernández Iñigo , Brzobohatý Břetislav , Ďurkovič Jaroslav
Published: October 2022

Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 × (Populus × canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.
Rootstock effects on bitter pit incidence in ‘Honeycrisp’ apples are associated with changes in fruit’s cell wall chemical properties
Authors: Islam Md Tabibul , Liu Jianyang , Das Protiva Rani , Singh Amritpal , Sherif Sherif M.
Published: October 2022

Bitter pit (BP) is a physiological disorder of apples that often appears during or after cold storage. Despite being defined as a calcium deficiency disorder, BP is a complex process that is not only affected by the total Ca2+ content in the fruit but also by the proper cellular Ca2+ homeostasis and partitioning. Early investigations have also suggested that rootstocks could affect BP development and severity. In the present study, rootstock effects on BP development were assessed on ‘Honeycrisp’ trees that were grafted on 14 different rootstocks (B.10, G.11, G.202, G.214, G.30, G.41, G.935, G.969, M.26 EMLA, M.9, V.1, V.5, V.6, and V.7). We evaluated BP incidence at harvest, and three months after cold storage for four, and three growing seasons, respectively. BP incidence was significantly reduced in ‘Honeycrisp’ trees on B.10 compared to other rootstocks, whereas trees on V.6 showed the highest percentage of BP at harvest and after cold storage. ‘Honeycrisp’ apples were collected from three different rootstocks (B.10, G.41, and V.6) two months after cold storage and evaluated for mineral nutrient composition, Ca2+ homeostasis, and cell wall properties, e.g., pectin content, pectin de-esterification rate and pectin methylesterase (PME) activity. Water-soluble and insoluble pectin content was markedly higher in fruits from B.10 than in G.41 and V.6. We also observed increased PME enzyme activity and a greater degree of water-insoluble pectin de-esterification in ‘Honeycrisp’ apples from V.6 compared to those from B.10. A significantly higher Ca2+ was found in the fruits from B.10 than G.41 and V.6. Higher Ca2+ and lower Mg2+ levels were also observed in the cell wall and water-insoluble pectin fractions of the fruits from B.10 compared to G.41 and V.6. However, the ratio of cell wall-bound Ca2+ to total Ca2+ was lower in B.10 compared to G.41 and V.6. Together, our results indicate that the tolerance of B.10 to BP could be attributed to a reduced PME activity and lower pectin de-esterification level, which in turn reduced the amount of Ca2+ cross-linked with pectin, and probably increased the apoplastic free calcium concentrations that is essential for maintaining cell membrane integrity and reducing BP development.
Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress
Authors: Zhang Fei , Lu Feng , Wang Yanqiu , Zhang Zhipeng , Wang Jiaxu , Zhang Kuangye , Wu Han , Zou Jianqiu , Duan Youhou , Ke Fulai , Zhu Kai
Published: October 2022

Sorghum is an important food crop with high salt tolerance. Therefore, studying the salt tolerance mechanism of sorghum has great significance for understanding the salt tolerance mechanism of C4 plants. In this study, two sorghum species, LRNK1 (salt-tolerant (ST)) and LR2381 (salt-sensitive (SS)), were treated with 180 mM NaCl salt solution, and their physiological indicators were measured. Transcriptomic and metabolomic analyses were performed by Illumina sequencing and liquid chromatography-mass spectrometry (LC-MS) technology, respectively. The results demonstrated that the plant height, leaf area, and chlorophyll contents in LRNK1 were significantly higher than in LR2381. Functional analysis of differently expressed genes (DEGs) demonstrated that plant hormone signal transduction (GO:0015473), carbohydrate catabolic processes (GO:0016052), and photosynthesis (GO:0015979) were the main pathways to respond to salt stress in sorghum. The genes of the two varieties showed different expression patterns under salt stress conditions. The metabolomic data revealed different profiles of salicylic acid and betaine between LRNK1 and LR2381, which mediated the salt tolerance of sorghum. In conclusion, LRNK1 sorghum responds to salt stress via a variety of biological processes, including energy reserve, the accumulation of salicylic acid and betaine, and improving the activity of salt stress-related pathways. These discoveries provide new insights into the salt tolerance mechanism of sorghum and will contribute to sorghum breeding.
Genome-wide identification and characterization of OVATE family proteins in Betula luminifera reveals involvement of BlOFP3 and BlOFP5 genes in leaf development
Authors: Borah Priyanka , Ni Fei , Ying Weiyang , Zhuang Hebi , Chong Sun-Li , Hu Xian-Ge , Yang Jun , Lin Er-pei , Huang Huahong
Published: October 2022

Ovate family proteins (OFP) are plant-specific transcription factors involved in regulating morphologies of the lateral organs, plant growth and development. However, the functional roles of OFP genes in Betula luminifera, an important timber tree species, are not well studied. In this study, we identified 20 BlOFP genes and analyzed their phylogenetic relationship, gene structure, conserved motifs, and cis-elements. Further, expression analysis indicates that BlOFP genes were up-regulated in leaves on the one-year-old branch compared to leaves on the current-year branch and bract, except BlOFP7, BlOFP11, BlOFP14 and BlOFP12. The overexpression of BlOFP3 and BlOFP5 in Arabidopsis thaliana not only resulted in a slower growth rate but also produced sawtooth shape, flatter and darker green rosette leaves. Further investigation showed that the leaf thickness of the transgenic plants was more than double that of the wild type, which was caused by the increasement in the number and size of palisade tissue cells. Furthermore, the expression analysis also indicated that the expressions of several genes related to leaf development were significantly changed in the transgene plants. These results suggested the significant roles of BlOFP3 and BlOFP5 in leaf development. Moreover, protein-protein interaction studies showed that BlOFP3 interacts with BlKNAT5, and BlOFP5 interacts with BlKNAT5, BlBLH6 and BlBLH7. In conclusion, our study demonstrates that BlOFP3 and BlOFP5 were involved in leaf shape and thickness regulation by forming a complex with BlKNAT5, BlBLH6 and BlBLH7. In addition, our study serves as a guide for future functional genomic studies of OFP genes of the B. luminifera.
CRISPR/Cas9-mediated genome editing of RsGL1a and RsGL1b in radish (Raphanus sativus L.)
Authors: Muto Naoki , Matsumoto Takashi
Published: October 2022

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a powerful tool widely used for genome editing in various organisms, including plants. It introduces and facilitates the study of rare genetic mutations in a short time and is a potent tool to assist in plant molecular breeding. Radish (Raphanus sativus L.) is an important Brassicaceae vegetable cultivated and consumed worldwide. However, the application of the CRISPR/Cas9 system is limited by the absence of an efficient transformation system in radish. This study aimed to establish a CRISPR/Cas9 system in radish employing the Agrobacterium-mediated genetic transformation system reported recently. For this purpose, we performed genome editing using the CRISPR/Cas9 system targeting the GLABRA1 (GL1) orthologs, RsGL1a and RsGL1b, that induces leaf trichome formation in radish. A Cas9/single guide RNA (sgRNA) vector with a common sgRNA corresponding to RsGL1a and RsGL1b was transferred. A total of eight T0 plants were analyzed, of which six (editing efficiency 75%) had a mutated RsGL1a, five (62.5%) had a mutated RsGL1b, and five showed mutations in both RsGL1a and RsGL1b. Most mutations in T0 plants were short (<3 bp) deletions or insertions, causing frameshift mutations that might produce non-functional proteins. Chimeric mutations were detected in several T0 generation plants. In the T1 generation, the hairless phenotype was observed only in plants with knockout mutations in both RsGL1a and RsGL1b. The majority of mutant alleles in T0 plants, with the exception of the chimeric mutant plants detected, were stably inherited in the T1 generation. In conclusion, we successfully knocked out RsGL1a and RsGL1b using the CRISPR/Cas9 system and demonstrated that both RsGL1a and RsGL1b independently contribute to the induction of leaf trichome formation in radish. In this study, genome-edited plants without T-DNA, which are useful as breeding material, were obtained. The findings prove the feasibility of genome editing in radish using a CRISPR/Cas9 system that could accelerate its molecular breeding to improve agronomically desirable traits.
Effect of nano-silicon on the regulation of ascorbate-glutathione contents, antioxidant defense system and growth of copper stressed wheat (Triticum aestivum L.) seedlings
Authors: Riaz Muhammad , Zhao Shaopeng , Kamran Muhammad , Ur Rehman Naveed , Mora-Poblete Freddy , Maldonado Carlos , Hamzah Saleem Muhammad , Parveen Aasma , Ahmed Al-Ghamdi Abdullah , Al-Hemaid Fahad M. , Ali Shafaqat , Elshikh Mohamed S.
Published: October 2022

Copper (Cu2+) toxicity can inhibit plant growth and development. It has been shown that silicon (Si) can relieve Cu2+ stress. However, it is unclear how Si-nanoparticles (SiNPs) relieve Cu2+ stress in wheat seedlings. Therefore, the current study was conducted by setting up four treatments: CK, SiNP: (2.5 mM), Cu2+: (500 µM), and SiNP+Cu2+: (2.5 mM SiNP+500 µM Cu2+) to explore whether SiNPs can alleviate Cu2+ toxicity in wheat seedlings. The results showed that Cu2+ stress hampered root and shoot growth and accumulated high Cu2+ concentrations in roots (45.35 mg/kg) and shoots (25.70 mg/kg) of wheat as compared to control treatment. Moreover, Cu2+ treatment inhibited photosynthetic traits and chlorophyll contents as well as disturbed the antioxidant defense system by accumulating malondialdehyde (MDA) and hydrogen peroxidase (H2O2) contents. However, SiNPs treatment increased root length and shoot height by 15.1% and 22%, respectively, under Cu2+ toxicity. Moreover, SiNPs application decreased MDA and H2O2 contents by 31.25% and 19.25%, respectively. SiNPs increased non-enzymatic compounds such as ascorbic acid-glutathione (AsA-GSH) and enhanced superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbic peroxidase (APX) activities by 77.5%, 141.7%, 68%, and 80%, respectively. Furthermore, SiNPs decreased Cu2+ concentrations in shoots by 26.2%, as compared to Cu2+ treatment alone. The results concluded that SiNPs could alleviate Cu2+ stress in wheat seedlings. The present investigation may help to increase wheat production in Cu2+ contaminated soils.
Sex-based metabolic and microbiota differences in roots and rhizosphere soils of dioecious papaya (Carica papaya L.)
Authors: Zhou Yongmei , Pang Ziqin , Yuan Zhaonian , Fallah Nyumah , Jia Haifeng , Ming Ray
Published: October 2022

Dioecious plant species have a high genetic variation that is important for coping with or adapting to environmental stress through natural selection. Intensive studies have reported dimorphism morphism in morphology, physiology, as well as biotic and abiotic stress responses in dioecious plants. Here, we demonstrated the dimorphism of metabolic profile and the preference of some microorganisms in the roots and rhizosphere soils of male and female papaya. The metabolic composition of roots were significantly different between the males and females. Some sex hormones occurred in the differential metabolites in roots and rhizosphere soils. For example, testosterone was up-regulated in male papaya roots and rhizosphere soils, whereas norgestrel was up-regulated in the female papaya roots, indicating a possible balance in papaya roots to control the sexual differentiation. Plant hormones such as BRs, JAs, SA and GAs were also detected among the differential metabolites in the roots and rhizosphere soils of dioecious papaya. In addition, some metabolites that have medicinal values, such as ecliptasaponin A, crocin, berberine and sapindoside A were also expressed differentially between the two sexes. Numerous differential metabolites from the papaya roots were secreted in the soil, resulting in the differences in microbial community structure in the roots and rhizosphere soils. Some nitrogen-fixing bacteria such as Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Brevundimonas and Microvirga were enriched in the male papaya roots or rhizosphere soils. While Candidatus Solibacter and Tumebacillus, which utilize organic matters, were enriched in the roots or rhizosphere soils of the female papaya. Some differences in the fungi abundance were also observed in both male and female papaya roots. These findings uncovered the effect of sex types on the metabolic and microbiota differences in roots and rhizosphere soils in papaya and will lead to investigations of underlining genomic and molecular mechanisms.
Grazing preference and isotopic contributions of kelp to Zostera marina mesograzers
Authors: Olson Angeleen M. , Prentice Carolyn , Monteith Zachary L. , VanMaanen Derek , Juanes Francis , Hessing-Lewis Margot
Published: October 2022

In seagrass food webs, small invertebrate mesograzers often exert top-down control on algal epiphytes growing on seagrass blades, which in turn releases the seagrass from competition for light and nutrients. Yet, nearshore habitat boundaries are permeable, and allochthonous subsidies can provide alternative food sources to in-situ production in seagrass meadows, which may in turn alter mesograzer-epiphyte interactions. We examined the contribution of allochthonous kelp (Nereocystis luetkeana), autochthonous epiphytic macroalgal (Smithora naiadum), Ulva lactuca, and seagrass production to mesograzer diets in a subtidal Zostera marina (eelgrass) meadow. In both choice feeding experiments and isotopic analysis, mesograzer diets revealed a preference for allochthonous N. luetkeana over Z. marina, S. naiadum, and U. lactuca. Notably, Idotea resecata showed an ~20x greater consumption rate for N. luetkeana in feeding experiments over other macrophytes. In the meadow, we found a positive relationship between epiphytic S. naiadum and gammarid amphipod biomass suggesting weak top-down control on the S. naiadum biomass. Epiphyte biomass may be driven by bottom-up factors such as environmental conditions, or the availability and preference of allochthonous kelp, though further work is needed to disentangle these interactions. Additionally, we found that gammarid and caprellid amphipod biomass were positively influenced by adjacency to kelp at seagrass meadow edges. Our findings suggest that N. luetkeana kelp subsidies are important to the diets of mesograzers in Z. marina meadows. Spatial planning and management of marine areas should consider trophic linkages between kelp and eelgrass habitats as a critical seascape feature if the goal is to conserve nearshore food web structure and function.
Substance accumulation of a wetland plant, Leersia japonica, during senescence in the Yihe and Shuhe River Basin, North China
Authors: Yang Xiuyi , Wang Guanqun , Lei Shutong , Li Zongfeng , Zeng Bo
Published: October 2022

Leersia japonica is a perennial Gramineae grass that is dominant in shallow wetlands of the Yihe and Shuhe River Basin, North China. Previous studies have shown that L. japonica recovers early (March), tillers strongly, and has an excellent ability to purify sewage in spring. This early revival might play a vital role in water purification function; however, whether the plant benefits from the physiological activities during senescence remains unclear. Therefore, in this study, an experiment was conducted during the winter of 2016 and in the following spring. Morphology (height, biomass, root morphology), physiology (root vitality, malondialdehyde [MDA], superoxide dismutase [SOD]), substance contents (soluble sugar, soluble protein) and substance transportation (activity of enzymes for transportation and energy supply) were determined during weeks 0, 2, 4, 6, and 8 of the senescence stage (October 11, 2016); as well as substance contents and bud increments during days 0,7, 14, 21, 31 and 41 of the revival period (February 22, 2017). The results revealed that (1) the root biomass of L. japonica increased significantly during senescence, even after the leaves withered. (2) The root diameter of L. japonica decreased significantly, while root weight per volume and root superficial area per volume increased significantly during senescence. The root vitality was relatively stable in winter, especially for root absorption area per volume. (3) No significant difference was observed in membrane stability of stems, rhizomes and roots of L. japonica in winter, with the MDA content remaining stable and SOD activity increasing significantly during senescence. (4) The soluble sugar content of all tissues of L. japonica increased sharply during senescence; while it decreased significantly in spring, especially for buds. (5) The enzymes for substance metabolism responded differently, with activities of H+-ATPase and pyruvate decarboxylase (PDC) decreasing, and alcohol dehydrogenase (ADH) increasing. Therefore, L. japonica has active morphological adaptation of roots, physiological regulation, and massive substance accumulation during senescence stage. The special life-history trait ensures L. japonica survival in winter and revival in early spring, which makes it being an excellent plant for purifying sewage in spring.
Genome-wide association studies provide genetic insights into natural variation of seed-size-related traits in mungbean
Authors: Liu Jinyang , Lin Yun , Chen Jingbin , Yan Qiang , Xue Chenchen , Wu Ranran , Chen Xin , Yuan Xingxing
Published: October 2022

Although mungbean (Vigna radiata (L.) R. Wilczek) is an important legume crop, its seed yield is relatively low. To address this issue, here 196 accessions with 3,607,508 SNP markers were used to identify quantitative trait nucleotides (QTNs), QTN-by-environment interactions (QEIs), and their candidate genes for seed length (SL), seed width, and 100-seed weight (HSW) in two environments. As a result, 98 QTNs and 20 QEIs were identified using 3VmrMLM, while 95, >10,000, and 15 QTNs were identified using EMMAX, GEMMA, and CMLM, respectively. Among 809 genes around these QTNs, 12 were homologous to known seed-development genes in rice and Arabidopsis thaliana, in which 10, 2, 1, and 0 genes were found, respectively, by the above four methods to be associated with the three traits, such as VrEmp24/25 for SL and VrKIX8 for HSW. Eight of the 12 genes were significantly differentially expressed between two large-seed and two small-seed accessions, and VrKIX8, VrPAT14, VrEmp24/25, VrIAR1, VrBEE3, VrSUC4, and Vrflo2 were further verified by RT-qPCR. Among 65 genes around these QEIs, VrFATB, VrGSO1, VrLACS2, and VrPAT14 were homologous to known seed-development genes in A. thaliana, although new experiments are necessary to explore these novel GEI-trait associations. In addition, 54 genes were identified in comparative genomics analysis to be associated with seed development pathway, in which VrKIX8, VrABA2, VrABI5, VrSHB1, and VrIKU2 were also identified in genome-wide association studies. This result provided a reliable approach for identifying seed-size-related genes in mungbean and a solid foundation for further molecular biology research on seed-size-related genes.
Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress in Nicotiana tabacum
Authors: Zhang Hui , Li Zefeng , Xu Guoyun , Bai Ge , Zhang Peipei , Zhai Niu , Zheng Qingxia , Chen Qiansi , Liu Pingping , Jin Lifeng , Zhou Huina
Published: October 2022

Proteins of the Nitrate Transporter 1/Peptide Transporter (NPF) family transport a diverse variety of substrates, such as nitrate, peptides, hormones and chloride. In this study, a systematic analysis of the tobacco (Nicotiana tabacum) NPF family was performed in the cultivated ‘K326’. In total, 143 NtNPF genes were identified and phylogenetically classified into eight subfamilies, NPF1 to NPF8, based on the classification of NPF families in other plant species. The chromosomal locations and structures of the NtNPF genes were analyzed. The expression profiles of NtNPF genes under NaCl stress were analyzed to screen the possible NPF genes involving in chloride regulation in tobacco. Most NtNPF6 genes responded to salt stress in the roots and leaves. The expression of NtNPF6.13 was significantly down-regulated after salt stress for 12h. The chloride content was reduced in the roots of ntnpf6.13 mutant. These findings support the participation of NtNPF6.13 in chloride uptake. Several other NtNPF genes that play potential roles in chloride metabolism of tobacco require further study.
A study of practical drawing skills and knowledge transferable skills of children based on STEAM education
Authors: Yu Lan , Li Yanfang
Published: October 2022

The STEAM education involves children’s ability to integrate and apply their knowledge of science, technology, engineering, arts, and mathematics. The application and transfer of interdisciplinary knowledge in practical activities is the structure of STEAM education. This study assesses children’s practical drawing skills and transferable skills based on the global features of their realistic figure drawing. The drawings incorporate the visual information and the multidisciplinary knowledge that children acquire. The assessment variables of the global features are observation perspectives, baseline, and comparison. The results showed that most children present their works through the front view. The children of different age groups show differences in express baseline and comparison features. Boys and girls show some variances in baseline features. Moreover, children are relatively unskilled at applying interdisciplinary knowledge in their drawings.
The influence of the literacy environment on children’s writing development in Chinese
Authors: Su Mengmeng , Fan Yi , Wu Jifeng , Qiao Bingyan , Zhou Wei
Published: October 2022

The present study investigated the influence of literacy environment on the performance of writing narratives for primary school students. Two hundred and fifty Chinese children participated in this study. There were 146 third graders (71 boys and 75 girls) and 104 fifth graders (53 boys and 51 girls). Results showed that children’s writing abilities differed at the word level and sentence level between third grade and fifth grade. Formal literacy experience (parent teaching of characters) predicted the writing performance of third graders, while informal literacy experience (the visiting frequency of various places) predicted the writing performance of fifth graders. After controlling the effect of reading efficiency on the writing skills, the prediction of formal and informal literacy experiences on the writing performance remained. The results suggest the importance of formal and informal literacy experiences on the writing development of primary school students.
Corporate social responsibility and workplace health promotion: A systematic review
Authors: Alonso-Nuez María-Jesús , Cañete-Lairla Miguel-Ángel , García-Madurga Miguel-Ángel , Gil-Lacruz Ana-Isabel , Gil-Lacruz Marta , Rosell-Martínez Jorge , Saz-Gil Isabel
Published: October 2022

The complex situation that global society is facing as a result of COVID-19 has highlighted the importance of companies committing to the principles of social responsibility. Among the internal initiatives, those related to the health of workers are, obviously, highly topical. The objective of our research is to provide concise knowledge of the relationship between workplace health promotion (WHP) and corporate social responsibility (CSR) so that the relevant specialized research was gathered in a single document that lays the foundations of its applicability. A systematic review, following the PRISMA method, has been carried out. Twenty-seven articles have been selected from the main scientific databases. Their qualitative analysis concludes that CSR and WHP are linked, have beneficial reciprocal effects, need committed leadership respectful of autonomy and voluntariness, and require the establishment of specific goals within the framework of the organizations' sustainability policies. Future studies should establish the impact of the pandemic on these aspects.
Creative thinking in the teaching of Chinese language and literature in colleges from the perspective of educational psychology
Authors: Zhong Li
Published: October 2022

In the major of Chinese language and literature, the reading of Chinese language and literature is the most basic part. With the development of internet technology, the teaching of Chinese language and literature has also ushered in new changes. In order to better innovate the teaching methods of Chinese language and literature education according to the emerging internet technology, this study applies the scaffolding teaching model based on educational psychology to Chinese language teaching. Firstly, the educational psychology and scaffolding teaching theory are analyzed, and a scaffolding teaching model of educational psychology is proposed. Secondly, this model is applied to the teaching of Chinese language and literature majors in colleges, and teaching experiments are carried out. The results show that the Chinese language and literature reading anxiety of the students in the experimental class has been alleviated to a certain extent. After using the scaffolding teaching model based on human-computer interaction and educational psychology to conduct teaching experiments, the average reading anxiety of the experimental class is 67.13 points, and the average reading anxiety of the control class is about 76.52 points. The average post-test reading anxiety of the experimental class is 9.39 points lower than that of the control class. The students’ Chinese language and literature scores improve after the experiment. Therefore, the scaffolding teaching model based on educational psychology can be used in the teaching of Chinese language and literature majors in colleges and universities. This study can realize the effective teaching inside and outside the classroom of Chinese language and literature in colleges and universities, promote the deep integration of educational psychology and the teaching of Chinese language and literature, and provide a new teaching idea for Chinese language and literature teachers.
Multidimensional relative poverty of rural women: Measurement, dynamics, and influencing factors in China
Authors: Peng Yan
Published: October 2022

Background

China has eliminated absolute poverty; however, relative poverty still exists. Specifically, the characteristic group of rural women is affected by traditional gender concepts and behavioral norms, and rural women are often relatively deprived of economics, rights, abilities, and information.

Objective

Therefore, studying their relative poverty is crucial for realizing the overall development of China and the shared prosperity of farmers.

Methods

This study utilizes the multidimensional poverty theory to construct an analytical framework for the multidimensional relative poverty of rural households using the Alkire-Foster multidimensional measurement method and the tracking survey data of the “China Family Tracking Survey” from 2010 to 2016. From the “individual perspective,” the six dimensions of economy, health, humanities, spiritual life, social relations, and rights are used to measure and compare the relative poverty of women and men in rural households in China.

Results

The results showed that the relative poverty of women in rural households is extensive and broader, especially in the economic, humanities, and rights dimensions, and is much higher than that of men. Education level, physical health, ideology, and family status are the primary factors affecting the multidimensional relative poverty of women.

Conclusion

This study finds that the relative poverty of rural women exists within the family and it is multi-dimensional. This finding provides a reference for promoting well-rounded human development and achieving common prosperity for everyone.
Relations between character strengths, school satisfaction, enjoyment of learning, academic self-efficacy, and school achievement: An examination of various aspects of positive schooling
Authors: Weber Marco , Harzer Claudia
Published: October 2022

This study is embedded in the theoretical framework of the engine model of positive schooling. Accordingly, relations were investigated between students’ endogenous input variables (i.e., character strengths), process variables (i.e., school satisfaction, enjoyment of learning, and academic self-efficacy), and school achievement as an outcome variable. A sample of 300 students (between 10 and 17 years of age) completed web-based self-report measures for all key variables. Specific character strengths (e.g., love of learning, zest, hope, perseverance, and perspective) were substantially positively related to school satisfaction, enjoyment of learning, academic self-efficacy, and/or school achievement. Exploratory mediation analyses supported the basic assumption that processes (i.e., school satisfaction, enjoyment of learning, and academic self-efficacy) mediate the relations between character strengths as input variables and school achievement as an outcome variable. The findings underline the benefit of studying inputs, processes, and outcomes simultaneously to better understand the interplay among relevant variables in the context of positive schooling.
Effects of the COVID-19 Pandemic on the Success of Traditional Small and Medium Enterprises (SMEs): An Investigation of the Footprints of Economic Crisis Attributable to COVID-19
Authors: Khaliq Anum , Ali Shunaid , Chen Ziyi , Kanwal Sehrish , Khan Farina , Niazi Abdul Aziz Khan , Chen Leping
Published: October 2022

The COVID-19 pandemic created a significant economic decline and altered market behavior, forcing buyers and dealers online. The traditional local market merchants are not fully equipped with e-commerce business techniques and strategies, which is a barrier to their e-commerce behavior and success. The study aims to help small-medium firms adapt to an uncertain economic environment instead of reducing or shutting down business-like in Pakistan. From health to education, economy to domestic and social protection, various researches have been done since 2020. The researcher used primary data sources and did a Quantitative study after collecting the 240 samples size of data from the successful e-commerce players of Pakistan. The results confirm that customer satisfaction is essential for entrepreneurs to succeed, as customers were not satisfied with online shopping during COVID-19. Customer knowledge management (CKM) and job satisfaction (JS) are studied as potential and realized capacity variables. CKM act as a strategic asset to collect and assimilate the external customer knowledge. In contrast, satisfied employees act as a valuable asset that dynamically responds to changing customer needs and business environment by efficiently utilizing their knowledge and skills and reaching business success which is mirrored in customer satisfaction. Conclusive results enable practitioners to perceive the business success during economic crises in the organization’s absorptive capacity.
Addictive disorders, psychiatric symptoms, and potentially traumatic events in individuals with disabilities
Authors: Marín-Navarrete Rodrigo , Sánchez-Domínguez Ricardo , Pérez-López Alejandro , Saracco-Alvarez Ricardo
Published: October 2022

Background/Objectives

Individuals with disabilities (IWD) have a higher risk of potentially traumatic events (PTEs) either in childhood or adulthood, increasing the risk of suicide attempts, mental disorders, and substance use disorder. The aim of this study was to explore the association between substance use, psychiatric symptoms and suicidal behavior with PTEs. A Multisite cross-sectional study was conducted.

Materials and methods

The sample includes 1,098 participants with any type of disability (motor, intellectual, visual, and mixed) located in Mexico City. Traumatic events, violence, discrimination, addictive disorders, and psychiatric disorders were examined. Multivariate logistic regression models were conducted. Data was collected between September–October 2014.

Results

People with motor or visual disability have a higher prevalence in nicotine use disorder (NUD), generalize anxiety disorder (GAD), mayor depression disorder (MDD), want to be dead, and lifetime suicide attempts. Intellectual disability group only presents GAD and MDD. All disability groups have a high prevalence of PTEs. Verbal violence in childhood, sexual abuse, discrimination and serious accidents had a strong impact in the development of NUD, psychiatric symptoms and suicidal behavior.

Conclusion

These findings show the relevance of develop specific tools for detection, referral and treatment, in order to improve the mental health of people with disabilities.
Taking gambles at face value: Effects of emotional expressions on risky decisions
Authors: Winkielman Piotr , Trujillo Jennifer L. , Bornemann Boris , Knutson Brian , Paulus Martin P.
Published: October 2022

Emotional facial expressions are ubiquitous and potent social stimuli that can signal favorable and unfavorable conditions. Previous research demonstrates that emotional expressions influence preference judgments, basic approach-avoidance behaviors, and reward learning. We examined whether emotional expressions can influence decisions such as choices between gambles. Based on theories of affective cue processing, we predicted greater risk taking after positive than negative expressions. This hypothesis was tested in four experiments across tasks that varied in implementation of risks, payoffs, probabilities, and temporal decision requirements. Facial expressions were presented unobtrusively and were uninformative about the choice. In all experiments, the likelihood of a risky choice was greater after exposure to positive versus neutral or negative expressions. Similar effects on risky choice occurred after presentation of different negative expressions (e.g., anger, fear, sadness, and disgust), suggesting involvement of general positive and negative affect systems. These results suggest that incidental emotional cues exert a valence-specific influence of on decisions, which could shape risk-taking behavior in social situations.