bluuebunny/crossref_metadata_2025 huggingface dataset:
A Telerehabilitation Approach to Chronic Facial Paralysis in the COVID-19 Pandemic Scenario: What Role for Electromyography Assessment?
Authors: de Sire Alessandro , Marotta Nicola , Agostini Francesco , Drago Ferrante Vera , Demeco Andrea , Ferrillo Martina , Inzitari Maria Teresa , Pellegrino Raffaello , Russo Ilaria , Ozyemisci Taskiran Ozden , Bernetti Andrea , Ammendolia Antonio
Published: March 2022

There is a lack of data on patient and diagnostic factors for prognostication of complete recovery in patients with peripheral facial palsy. Thus, the aim of this study was to evaluate the role of a telerehabilitave enhancement through the description of a case report with the use of short-wave diathermy and neuromuscular electrical stimulation combined to facial proprioceptive neuromuscular facilitation (PNF) rehabilitation in unrecovered facial palsy, in a COVID-19 pandemic scenario describing a paradigmatic telerehabilitation report. A 43-year-old woman underwent a facial rehabilitation plan consisting of a synergistic treatment with facial PNF rehabilitation, short-wave diathermy, and neuromuscular electrical stimulation (12 sessions lasting 45 min, three sessions/week for 4 weeks). Concerning the surface electromyography evaluation of frontal and orbicularis oris muscles, the calculated ratio between amplitude of the palsy side and normal side showed an improvement in terms of movement symmetry. At the end of the outpatient treatment, a daily telerehabilitation protocol with video and teleconsultation was provided, showing a further improvement in the functioning of a woman suffering from unresolved facial paralysis. Therefore, an adequate telerehabilitation follow-up seems to play a fundamental role in the management of patients with facial palsy.
Optimal Control Strategies for the Premium Policy of an Insurance Firm with Jump Diffusion Assets and Stochastic Interest Rate
Authors: Guerdouh Dalila , Khelfallah Nabil , Vives Josep
Published: March 2022

In this paper, we present a stochastic optimal control model to optimize an insurance firm problem in the case where its cash-balance process is assumed to be described by a stochastic differential equation driven by Teugels martingales. Noticing that the insurance firm is able to control its cash-balance dynamics by regulating the underlying premium rate, the aim of the policy maker is to select an appropriate premium in order to minimize the total deviation of the state process to some pre-set target level. As a part of stochastic maximum principle approach, a verification theorem is used to fulfill this achievement.
Evaluating Impact of Farmland Recessive Morphology Transition on High-Quality Agricultural Development in China
Authors: Lu Xinhai , Li Zhoumi , Wang Hongzheng , Tang Yifeng , Hu Bixia , Gong Mingyue , Li Yulong
Published: March 2022

Recessive morphology transition (RMT) involves the smooth transition of farmland property rights, input structure, quality, and function. China’s agriculture has changed from a period of high-speed growth to a period of high-quality development. Compared with dominant morphology transition (DMT) characterized by quantitative focus, it is of more practical significance to explore the impact of RMT on high-quality agricultural development (HAD). This paper firstly constructed a multidimensional index system to quantify HAD. Based on analysis of the impact mechanism of RMT on HAD, a spatial econometric model was established to explore the impact by making use of the panel data of 27 provinces in China from 2003–2017. The results indicated that RMT and HAD both have positive geospatial correlation. Furthermore, the spatial econometric model provides more accurate results of the impact of RMT on HAD than panel models. If the RMT in a local province increases by 1%, HAD could be augmented by 0.13%. Likewise, RMT has a strong positive spatial spillover effect on HAD. If the RMT in a certain province increases by 1%, HAD could add 1.22% in neighboring provinces. The analysis suggests that spatial coordination of farmland use is an important foundation for constructing high-quality development association of regional agriculture. It is necessary to strengthen intergovernmental cooperation in the process of farmland recessive morphology transition and high-quality agricultural development.
Investigating Change in the Willingness to Pay for a More Sustainable Tourist Destination in a World Heritage City
Authors: Jurado-Rivas Carlos , Sánchez-Rivero Marcelino
Published: March 2022

The willingness to pay for sustainable tourism products and services has been widely discussed in the scientific literature. However, change in the willingness to pay over time has rarely been analysed. Such studies are important for understanding the impact that the increasing debate on sustainability, including environmental, economic, and social aspects, may have had on the willingness of tourists to pay more in order to ensure the sustainability of destinations. The aim of this article is to assess how the willingness of tourists to pay for sustainable tourism services has developed in the Spanish city of Cáceres, declared a World Heritage Site in 1986. Data from surveys conducted in 2012 and 2016 were used for this purpose. Logistic regression was applied to determine whether sociodemographic characteristics of tourists who visit the city influenced their willingness to pay in each year. A Chow test was applied to elucidate whether the differences between the years were statistically significant. The results obtained indicate that only the level of education determined willingness to pay, while origin, gender and age showed no effect. No significant change in the willingness to pay for sustainability was found among tourists in Cáceres between 2012 and 2016. These findings indicate that the willingness to pay for more sustainable tourism services did not increase during the period studied. We propose some measures such as a ‘municipal observatory of sustainable tourism’ in order to increase willingness to pay in this type of destination.
Soil OC and N Stocks in the Saline Soil of Tunisian Gataaya Oasis Eight Years after Application of Manure and Compost
Authors: Brahim Nadhem , Ibrahim Hatem , Mlih Rawan , Bouajila Abdelhakim , Karbout Nissaf , Bol Roland
Published: March 2022

Soil organic matter plays an important role in improving soil properties, crop productivity and is a key constituent and driver of the global carbon cycle. Nevertheless, relatively limited quantitative information is available on the organic carbon (OC) stocks and the actual potentials for OC and total nitrogen (N) sequestration under arid cropping systems. In this study, we evaluated the immediate and long-term (after eight years) effects of compost or manure additions, at a rate of 100 t ha−1, on the soil OC and N stocks in the Gataaya oasis in Southern Tunisia. The oasis had been abandoned and no additions had taken place in the 10 years prior to experiment. Soil samples were taken systematically every 10 cm up to a depth of 50 cm. After adding compost (CMP) and manure (MAN) in 2013, the bulk density (BD) decreased in the surface layers, especially at the 0–10 cm soil layer where it declined from 1.53 g cm−3 to 1.38 g cm−3 under compost and 1.41 g cm−3 under manure. Soil OC and N stocks, however, increased after adding compost and manure. Manure contributed more to OC stock increase than compost, with +337 and +241%, respectively. Correspondingly, the N stock increased by + 47 and +12%, respectively, due to manure and compost. After four years, compared to 2013 stocks, the decrease in OC stock was almost identical with −43 (CMP) and −41% (MAN). However, N stock seemed more stable under compost compared to manure, with −2 and −19%, respectively. After eight years, the N stock remained higher in the deepest layer 30–50 cm compared to other layers. This suggested that high gypsum application can inhibit N mineralization. The initial enhanced OC stock after the organic amendment, both for compost and for manure, was very quickly lost and after eight years had virtually returned to the initial OC state by the end of the eight years. Therefore, these oasis ecosystems require a near annual supply of exogenous organic material to maintain OC at an enhanced level. After eight years, manure amendment was found to be better than compost for increasing soil OC (3.16 against 1.86 t/ha, respectively) and for increasing N (0.35 against 0.18 t/ha, respectively). However, the cost and availability make the amendment with compost more interesting in oasis (400 Tunisian dinars/t for compost against 1016 Tunisian dinars/t for manure).
Locating the Concept of Vulnerability in Canada’s Refugee Policies at Home and Abroad
Authors: Anderson Melissa Mary , Soennecken Dagmar
Published: March 2022

How is the concept of “vulnerability” employed in Canadian immigration law? This article presents findings from research conducted as part of the VULNER project (2019–23). We analyze how vulnerability is operationalized in Canada’s inland refugee (or asylum) determination procedures compared to its overseas resettlement program by first discussing some general principles, followed by an examination of the treatment of women and LGBTQI+ individuals seeking protection. We show that legal-bureaucratic logics have constructed two very heterogeneous worlds of vulnerability that may reproduce and exacerbate vulnerabilities rather than create a more inclusive, equitable protection regime in law, policy, and practice.
Management Strategies to Mitigate N2O Emissions in Agriculture
Authors: Hassan Muhammad Umair , Aamer Muhammad , Mahmood Athar , Awan Masood Iqbal , Barbanti Lorenzo , Seleiman Mahmoud F. , Bakhsh Ghous , Alkharabsheh Hiba M. , Babur Emre , Shao Jinhua , Rasheed Adnan , Huang Guoqin
Published: March 2022

The concentration of greenhouse gases (GHGs) in the atmosphere has been increasing since the beginning of the industrial revolution. Nitrous oxide (N2O) is one of the mightiest GHGs, and agriculture is one of the main sources of N2O emissions. In this paper, we reviewed the mechanisms triggering N2O emissions and the role of agricultural practices in their mitigation. The amount of N2O produced from the soil through the combined processes of nitrification and denitrification is profoundly influenced by temperature, moisture, carbon, nitrogen and oxygen contents. These factors can be manipulated to a significant extent through field management practices, influencing N2O emission. The relationships between N2O occurrence and factors regulating it are an important premise for devising mitigation strategies. Here, we evaluated various options in the literature and found that N2O emissions can be effectively reduced by intervening on time and through the method of N supply (30–40%, with peaks up to 80%), tillage and irrigation practices (both in non-univocal way), use of amendments, such as biochar and lime (up to 80%), use of slow-release fertilizers and/or nitrification inhibitors (up to 50%), plant treatment with arbuscular mycorrhizal fungi (up to 75%), appropriate crop rotations and schemes (up to 50%), and integrated nutrient management (in a non-univocal way). In conclusion, acting on N supply (fertilizer type, dose, time, method, etc.) is the most straightforward way to achieve significant N2O reductions without compromising crop yields. However, tuning the rest of crop management (tillage, irrigation, rotation, etc.) to principles of good agricultural practices is also advisable, as it can fetch significant N2O abatement vs. the risk of unexpected rise, which can be incurred by unwary management.
Trehalose Activates Hepatic and Myocardial Autophagy and Has Anti-Inflammatory Effects in db/db Diabetic Mice
Authors: Korolenko Tatiana A. , Ovsyukova Marina V. , Bgatova Nataliya P. , Ivanov Igor D. , Makarova Svetlana I. , Vavilin Valentin A. , Popov Alexey V. , Yuzhik Ekaterina I. , Koldysheva Elena V. , Korolenko Erik C. , Zavjalov Evgeny L. , Amstislavskaya Tamara G.
Published: March 2022

Db/db mice (carrying a mutation in the gene encoding leptin receptor) show autophagy suppression. Our aim was to evaluate the effect of autophagy inducer trehalose on liver and heart autophagy in db/db mice and to study inflammation dysregulation and the suitability of chitinases’ expression levels as diabetes markers. Thirty-eight male db/db mice and C57/BL mice (control) were used. The db/db model manifested inflammation symptoms: overexpression of TNF-α in the spleen and underexpression of IL-10 in the liver and spleen (cytokine imbalance). Simultaneously, we revealed decreased expression of chitotriosidase (CHIT1) and acid mammalian chitinase (CHIA) in the liver of db/db mice. CHIA expression in db/db mice is significantly lower only in the spleen. Trehalose treatment significantly reduced blood glucose concentration and glycated hemoglobin. Treatment of db/db mice by trehalose was followed by increased autophagy induction in the heart and liver (increased autolysosomes volume density studied by morphometric electron-microscopic method). Trehalose exerted beneficial cardiac effects possibly via increased lipophagy (uptake of lipid droplets). The autophagy activation by trehalose had several positive effects on the heart and liver of db/db mice; therefore, lipophagy activation seems to be a promising therapy for diabetes.
Tree Reconciliation Methods for Host-Symbiont Cophylogenetic Analyses
Authors: Libeskind-Hadas Ran
Published: March 2022

Phylogenetic reconciliation is a fundamental method in the study of pairs of coevolving species. This paper provides an overview of the underlying theory of reconciliation in the context of host-symbiont cophylogenetics, identifying some of the major challenges to users of these methods, such as selecting event costs and selecting representative reconciliations. Next, recent advances to address these challenges are discussed followed by a discussion of several established and recent software tools.
Determination of the Respiratory Compensation Point by Detecting Changes in Intercostal Muscles Oxygenation by Using Near-Infrared Spectroscopy
Authors: Contreras-Briceño Felipe , Espinosa-Ramirez Maximiliano , Keim-Bagnara Vicente , Carreño-Román Matías , Rodríguez-Villagra Rafael , Villegas-Belmar Fernanda , Viscor Ginés , Gabrielli Luigi , Andía Marcelo E. , Araneda Oscar F. , Hurtado Daniel E.
Published: March 2022

This study aimed to evaluate if the changes in oxygen saturation levels at intercostal muscles (SmO2-m.intercostales) assessed by near-infrared spectroscopy (NIRS) using a wearable device could determine the respiratory compensation point (RCP) during exercise. Fifteen healthy competitive triathletes (eight males; 29 ± 6 years; height 167.6 ± 25.6 cm; weight 69.2 ± 9.4 kg; V˙O2-máx 58.4 ± 8.1 mL·kg−1·min−1) were evaluated in a cycle ergometer during the maximal oxygen-uptake test (V˙O2-máx), while lung ventilation (V˙E), power output (watts, W) and SmO2-m.intercostales were measured. RCP was determined by visual method (RCPvisual: changes at ventilatory equivalents (V˙E·V˙CO2−1, V˙E·V˙O2−1) and end-tidal respiratory pressure (PetO2, PetCO2) and NIRS method (RCPNIRS: breakpoint of fall in SmO2-m.intercostales). During exercise, SmO2-m.intercostales decreased continuously showing a higher decrease when V˙E increased abruptly. A good agreement between methods used to determine RCP was found (visual vs NIRS) at %V˙O2-máx, V˙O2, V˙E, and W (Bland-Altman test). Correlations were found to each parameters analyzed (r = 0.854; r = 0.865; r = 0.981; and r = 0,968; respectively. p < 0.001 in all variables, Pearson test), with no differences (p < 0.001 in all variables, Student’s t-test) between methods used (RCPvisual and RCPNIRS). We concluded that changes at SmO2-m.intercostales measured by NIRS could adequately determine RCP in triathletes.
Alkanes as Membrane Regulators of the Response of Early Membranes to Extreme Temperatures
Authors: Misuraca Loreto , Caliò Antonino , LoRicco Josephine G. , Hoffmann Ingo , Winter Roland , Demé Bruno , Peters Judith , Oger Philippe M.
Published: March 2022

One of the first steps in the origin of life was the formation of a membrane, a physical boundary that allowed the retention of molecules in concentrated solutions. The proto-membrane was likely formed by self-assembly of simple readily available amphiphiles, such as short-chain fatty acids and alcohols. In the commonly accepted scenario that life originated near hydrothermal systems, how these very simple membrane bilayers could be stable enough in time remains a debated issue. We used various complementary techniques such as dynamic light scattering, small angle neutron scattering, neutron spin-echo spectroscopy, and Fourier-transform infrared spectroscopy to explore the stability of a novel protomembrane system in which the insertion of alkanes in the midplane is proposed to shift membrane stability to higher temperatures, pH, and hydrostatic pressures. We show that, in absence of alkanes, protomembranes transition into lipid droplets when temperature increases; while in presence of alkanes, membranes persist for longer times in a concentration-dependent manner. Proto-membranes containing alkanes are stable at higher temperatures and for longer times, have a higher bending rigidity, and can revert more easily to their initial state upon temperature variations. Hence, the presence of membrane intercalating alkanes could explain how the first membranes could resist the harsh and changing environment of the hydrothermal systems. Furthermore, modulating the quantity of alkanes in the first membranes appears as a possible strategy to adapt the proto-membrane behavior according to temperature fluctuations, and it offers a first glimpse into the evolution of the first membranes.
The Possible Role of the Type I Chaperonins in Human Insulin Self-Association
Authors: Pizzo Federica , Mangione Maria Rosalia , Librizzi Fabio , Manno Mauro , Martorana Vincenzo , Noto Rosina , Vilasi Silvia
Published: March 2022

Insulin is a hormone that attends to energy metabolism by regulating glucose levels in the bloodstream. It is synthesised within pancreas beta-cells where, before being released into the serum, it is stored in granules as hexamers coordinated by Zn2+ and further packaged in microcrystalline structures. The group I chaperonin cpn60, known for its assembly-assisting function, is present, together with its cochaperonin cpn10, at each step of the insulin secretory pathway. However, the exact function of the heat shock protein in insulin biosynthesis and processing is still far from being understood. Here we explore the possibility that the molecular machine cpn60/cpn10 could have a role in insulin hexameric assembly and its further crystallization. Moreover, we also evaluate their potential protective effect in pathological insulin aggregation. The experiments performed with the cpn60 bacterial homologue, GroEL, in complex with its cochaperonin GroES, by using spectroscopic methods, microscopy and hydrodynamic techniques, reveal that the chaperonins in vitro favour insulin hexameric organisation and inhibit its aberrant aggregation. These results provide new details in the field of insulin assembly and its related disorders.
Study on “Atypical” Migraine Auras in the Pediatric Age: The Role of Cortical Spreading Depression and the Physiopathogenetic Hypothesis Arising from Our Clinical Cases
Authors: Raieli Vincenzo , Capizzi Mariarita , Marino Antonio , Di Nardo Giovanni , Raucci Umberto , Parisi Pasquale
Published: March 2022

Migraine is a complex neurologic disorder by which several systems of the central nervous system (autonomous system, affective, cognitive, sensory and motor system) may be affected on different levels. About a fourth of migraine patients have migraine auras. The most common aura is the visual aura followed by the sensorial aura but motor deficits, as well as deficits of higher cortical centers (disorders of thinking, orientation, coherence, or concentration), may occur as well. In analogy with a headache diary, an aura diary can deliver important help in the diagnostic process of rare migraine manifestations and prevent the under-diagnosis of unusual migraine manifestations. Complex migraine manifestations are a diagnosis of exclusion, and a broad diagnostic work-up is necessary in order to exclude dangerous neurologic pathologies. In addition, here, we discuss the atypical clinical presentation and possible physio-pathogenetic related aspects of these atypical migraine aura features in the developmental age. In addition, we wanted to stress and analyze the clinical aspects of our children/adolescents with atypical auras, which seem to be more difficult to frame with the mechanisms originally proposed to explain the physio-pathogenetic relationship between CSD and aura. Finally, we discuss in detail the complex aspects of this topic on the basis of available data and propose new terminology: “Multiple, Synchronous and Asynchronous, Cortical and Subcortical Spreading Depression”.
Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
Authors: Dauda Wadzani Palnam , Abraham Peter , Glen Elkanah , Adetunji Charles Oluwaseun , Ghazanfar Shakira , Ali Shafaqat , Al-Zahrani Majid , Azameti Mawuli Kwamla , Alao Sheik Emmanuel Laykay , Zarafi Afiniki Bawa , Abraham Maryam Peter , Musa Hannatu
Published: March 2022

Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and secondary metabolism in organisms. Aspergillus species are among the most economically important fungal organisms in human medicine, industry, and agriculture worldwide. Exploring insight on the genome-wide annotations of cytochrome P450s in Aspergillus species is necessary for their biosynthetic applications. In this present study, we report the identification of 306 cytochrome P450s and their robust profiling in eight notable Aspergillus species (A. carbonarius, A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, and A. terreus). Based on the evolutionary relationship, the Aspergillus P450s families clustered into 15 clades, with clades V, I, and XIII recording higher percentages (17.3%, 15.00%, and 14.71%, respectively) of Cyp families. Cyps were classified into 120 families 64 clans, and their putative functions were also elucidated. P450s were predicted to be located in 13 subcellular components, but the endoplasm reticulum was the dominant location across the eight Aspergillus species. Cyps genes of Aspergillus species were associated with seven secondary metabolism-related gene clusters. Elucidating the genome-wide annotations of P450s enzymes in Aspergillus species will form vital potential biotechnological tools that could be harnessed for industrial, pharmaceutical, and agricultural use.
Cardiac Events in Childhood Cancer Survivors Treated with Anthracyclines: The Value of Previous Myocardial Strain Measurement
Authors: Pourier Milanthy , Merkx Remy , Loonen Jacqueline , van Cleef Alyssa , de Korte Chris , Bellersen Louise , Kapusta Livia , Mavinkurve-Groothuis Annelies
Published: March 2022

In echocardiographic surveillance of anthracycline-treated childhood cancer survivors (CCS), left ventricular ejection fraction (LVEF) has insufficient prognostic value for future cardiac events, whereas longitudinal strain may be more sensitive. We describe the long-term incidence of cardiac events in CCS after previous measurement of LVEF and myocardial strain. Echocardiography, including four-chamber view longitudinal strain (4CH-LS), of 116 anthracycline-treated CCS was obtained between 2005–2009 (index echocardiography). Follow-up was obtained at the late-effects clinic. Primary outcome was occurrence of cardiac events, defined as either symptomatic heart failure, life-threatening arrhythmias, LVEF < 40% or cardiac death, in CCS with normal versus abnormal index 4CH-LS. LVEF from subsequent echocardiograms was obtained to evaluate its natural course as a secondary outcome. After index echocardiography (median 13.1 years since childhood cancer diagnosis), our study added a median follow-up of 11.3 years (median last clinical contact 23.6 years since diagnosis). Only three CCS developed a cardiac event (6.2, 6.4 and 6.7 years after index echocardiography), resulting in a ten-year cumulative incidence of 2.7% (95%CI 0.9–8.2). All three CCS had a clearly reduced index 4CH-LS and relevant cardiovascular risk factors, whereas their index LVEFs were around the lower limit of normal. Index LVEF correlated with index 4CH-LS but mean long-term natural course of LVEF was comparable for CCS with abnormal versus normal index 4CH-LS. Absolute 10-year cumulative incidence of cardiac events in anthracycline-treated CCS during long-term follow-up was low. Sensitive echocardiographic measurements, such as 4CH-LS may be useful to tailor surveillance frequency in a selected group of CCS without cardiovascular disease.
The Anti-Genotoxic Activity of Wastewaters Produced after Water-Steam Distillation of Bulgarian Rosa damascena Mill. and Rosa alba L. Essential Oils
Authors: Gateva Svetla , Jovtchev Gabriele , Angelova Tsveta , Dobreva Ana , Mileva Milka
Published: March 2022

The steam distillation of valuable rose essential oil from R. damascena Mill. and R. alba L. generates large volumes of wastewaters. Although such wastewaters are bio-pollutants, they contain valuable bioactive compounds. In this study we investigated the cytotoxic/genotoxic and anti-cytotoxic/anti-genotoxic potential of these products. We used cytogenetic methods for induction of chromosome aberrations and micronuclei in two different experimental test-systems: ahigher plant and human lymphocyte cultures. Different experimental schemes of treatment with the waste products showed that the genotoxic activity of wastewater from the distillation of oils from R. alba and R. damascena was low in both test–systems. Human lymphocytes showed a higher sensitivity to the products than plant cells. Both types of waste products manifested anti-genotoxic effect against N-methyl-N′-nitro-N-nitrosoguanidine, a direct mutagen. The wastewaters obtained from steam distillation of rose essential oil have cytoprotective/genoprotective effect and could decrease DNA damage. Data are promising for further use of these products in pharmacy and other areas of human life.
Comparison of Auxenochlorella protothecoides and Chlorella spp. Chloroplast Genomes: Evidence for Endosymbiosis and Horizontal Virus-like Gene Transfer
Authors: Park Sang-Hyuck , Kyndt John A. , Brown Judith K.
Published: March 2022

Resequencing of the chloroplast genome (cpDNA) of Auxenochlorella protothecoides UTEX 25 was completed (GenBank Accession no. KC631634.1), revealing a genome size of 84,576 base pairs and 30.8% GC content, consistent with features reported for the previously sequenced A. protothecoides 0710, (GenBank Accession no. KC843975). The A. protothecoides UTEX 25 cpDNA encoded 78 predicted open reading frames, 32 tRNAs, and 4 rRNAs, making it smaller and more compact than the cpDNA genome of C. variabilis (124,579 bp) and C. vulgaris (150,613 bp). By comparison, the compact genome size of A. protothecoides was attributable primarily to a lower intergenic sequence content. The cpDNA coding regions of all known Chlorella species were found to be organized in conserved colinear blocks, with some rearrangements. The Auxenochlorella and Chlorella species genome structure and composition were similar, and of particular interest were genes influencing photosynthetic efficiency, i.e., chlorophyll synthesis and photosystem subunit I and II genes, consistent with other biofuel species of interest. Phylogenetic analysis revealed that Prototheca cutis is the closest known A. protothecoides relative, followed by members of the genus Chlorella. The cpDNA of A. protothecoides encodes 37 genes that are highly homologous to representative cyanobacteria species, including rrn16, rrn23, and psbA, corroborating a well-recognized symbiosis. Several putative coding regions were identified that shared high nucleotide sequence identity with virus-like sequences, suggestive of horizontal gene transfer. Despite these predictions, no corresponding transcripts were obtained by RT-PCR amplification, indicating they are unlikely to be expressed in the extant lineage.
A Simplified Approach for the Corrosion Fatigue Assessment of Steel Structures in Aggressive Environments
Authors: Milone Aldo , Landolfo Raffaele
Published: March 2022

Fatigue performance is often a key aspect when dealing with existing steel structures such as steel bridges or offshore constructions. This issue proves to be more critical as these structures are usually located in aggressive environments and are thus exposed to progressive degradation. Indeed, disruptive phenomena such as corrosion can severely worsen the fatigue performance of the steel components. Currently, the normative standards do not provide a codified procedure for the fatigue checks of steel structures subjected to ongoing corrosion. Within this framework, in this paper a simplified approach for the life-cycle assessment of corroded steel structures is proposed. For this purpose, the concept of “critical corrosion degree” is introduced, allowing the expression of corrosion fatigue checks in a more direct “demand vs. capacity” form with respect to the currently available methods. A first validation of such methodology is reported for the corrosion fatigue tests drawn from the literature. The predicted levels of critical corrosion are in good agreement with the values of artificially induced corrosion (i.e., 4, 8, and 12% of mass loss, respectively), with a maximum relative error of ≈9.3% for the most corroded specimen. Finally, parametrical analyses are performed, highlighting the influence of the model parameters on the corrosion fatigue performance of the steel elements.
Synthesis of In Situ ZrB2-SiC-ZrC Coating on ZrC-SiC Substrate by Reactive Plasma Spraying
Authors: Ma Bao-Xia , Wang Yang , Zhao Si-Cong , Wu Hao-Nan , Qiao Yang
Published: March 2022

In situ synthesis feasibility of ZrB2-SiC-ZrC composite coatings on ZrC ceramics by reactive plasma spraying (RPS) was investigated. To help to understand the phase evolution during plasma spraying process, reaction behavior in the ZrH2-Si-B4C system was explored carefully by differential scanning calorimetry. The results indicated that the phase transformation sequence in the ZrH2-Si-B4C system could be described as ZrH1.66, Zr3O, ZrC, ZrB2, Zr2Si, ZrSi, and SiC. The prior formation of ZrC was due to high diffusion rate of C atoms from B4C. ZrB2 was produced above 1100 °C. As the temperature increased, SiC were finally formed by the reaction of ZrC with ZrSi and B4C. The RPS composite coatings mainly consisted of ZrB2, SiC, and ZrC phases, except for a small fraction of ZrO2 phase. The microstructural characterization exhibited more dense melted splats, which appears to increase gradually with the increase in spraying currents and distances. The coatings had typical lamellar structure and adhered to the substrate well. The microhardness values were higher than 1000 HV1, but there were few variations with varying spraying currents and distances.
Attachment and Osteogenic Potential of Dental Pulp Stem Cells on Non-Thermal Plasma and UV Light Treated Titanium, Zirconia and Modified PEEK Surfaces
Authors: Guo Linna , Zou Ziang , Smeets Ralf , Kluwe Lan , Hartjen Philip , Gosau Martin , Henningsen Anders
Published: March 2022

Ultraviolet (UV) light and non-thermal plasma (NTP) treatment are chairside methods that can efficiently improve the biological aging of implant material surfaces caused by customary storage. However, the behaviors of stem cells on these treated surfaces of the implant are still unclear. This study aimed to investigate the effects of UV light and NTP treated surfaces of titanium, zirconia and modified polyetheretherketone (PEEK, BioHPP) on the attachment and osteogenic potential of human dental pulp stem cells (DPSCs) in vitro. Machined disks were treated using UV light and argon or oxygen NTP for 12 min each. Untreated disks were set as controls. DPSCs were cultured from the wisdom teeth of adults that gave informed consent. After 24 h of incubation, the attachment and viability of cells on surfaces were assessed. Cells were further osteogenically induced, alkaline phosphatase (ALP) activity was detected via a p-Nitrophenyl phosphate assay (day 14 and 21) and mineralization degree was measured using a Calcium Assay kit (day 21). UV light and NTP treated titanium, zirconia and BioHPP surfaces improved the early attachment and viability of DPSCs. ALP activity and mineralization degree of osteoinductive DPSCs were significantly increased on UV light and NTP treated surfaces of titanium, zirconia and also oxygen plasma treated Bio-HPP (p < 0.05). In conclusion, UV light and NTP treatments may improve the attachment of DPSCs on titanium, zirconia and BioHPP surfaces. Osteogenic differentiation of DPSCs can be enhanced on UV light and NTP treated surfaces of titanium and zirconia, as well as on oxygen plasma treated Bio-HPP.
Frost Resistance Investigation of Fiber-Doped Cementitious Composites
Authors: Ji Yongcheng , Zou Yunfei , Ma Yulong , Wang Haoxiang , Li Wei , Xu Wenyuan
Published: March 2022

Fibers used as reinforcement can increase the mechanical characteristics of engineering cementitious composites (ECC), but their frost resistance has received less attention. The mechanical properties of various fiber cementitious materials under the dual factors of freeze-thaw action and fiber dose are yet to be determined. This study examines the performance change patterns of cementitious composites, which contain carbon fiber, glass fiber, and polyvinyl alcohol (PVA) fiber at 0%, 0.5%, and 1% volume admixture in freeze-thaw tests. Three fiber cement-based materials are selected to do the compression and bending testing, and ABAQUS finite element modeling is used to assess the performance of fiber cement-based composite materials. The microscopic observation results show that the dispersion of glass and PVA fibers is higher than that of carbon fibers. As a result, the mechanical characteristics of the fiber-doped cementitious composites increase dramatically after freeze-thaw with increasing dosage. The compression test results show the frost resistance of carbon fiber > PVA fiber > glass fiber. In addition, the bending test results show the frost resistance of carbon fiber > glass fiber > PVA fiber. The 3D surface plots of the strength changes are established to observe the mechanical property changes under the coupling effect of admixture and freeze-thaw times. ABAQUS modeling is used to predict the strength of the cementitious composites under various admixtures and freeze-thaw cycles. The bending strength numerical equation is presented, and the bending and compressive strengths of three different fiber-cement matrix materials are accurately predicted.
Experimental Investigation on the Vertical Ductility of Rectangular CFST Columns Loaded Axially
Authors: Grzeszykowski Bartosz , Szmigiera Elżbieta Danuta
Published: March 2022

A total of 5 steel and 21 rectangular composite concrete-filled steel tube (CFST) columns of moderate slenderness were tested to investigate their ductility under axial compression. The importance of the vertical ductility of columns was discussed, and a novel ductility measure was proposed and utilized to examine the ductility of tested specimens. The analyses showed that the ductility of axially compressed CFST columns highly depends on their failure mode. The key feature influencing the ductility is their ability to dissipate the energy of imposed loads. The larger the volume of a material that may permanently deform and consequently dissipate the energy, the greater this ability. In consequence, the ductility of specimens exhibiting local failure mode was higher in comparison to the columns that underwent global or mixed global—local failure. It was found that both steel and composite columns were able to carry axial loads in the post-critical state; but due to the limitations of local buckling of the steel cross-section in the concrete core and concrete confinement, all tested composite columns showed greater ductility than their steel counterparts.
Fracture Behavior of Steel Slag Powder-Cement-Based Concrete with Different Steel-Slag-Powder Replacement Ratios
Authors: Zhuo Ke-Xian , Liu Guo-Tao , Lan Xue-Wei , Zheng Dong-Ping , Wu Si-Quan , Wu Pei-Zong , Guo Yong-Chang , Lin Jia-Xiang
Published: March 2022

The influence of different replacement ratios of steel-slag powder as cement-replacement material on the fracture performance of concrete is studied in this paper. A three-point bending fracture test is carried out on slag powder-cement-based concrete (SPC)-notched beams with steel-slag powder as cementitious materials, partially replacing cement (0%, 5%, 10%, 15%, and 20%). Load-deflection curves and load-crack-opening displacement curves of SPC-notched beams with five different replacement ratios of steel-slag powder were obtained. The effects of different steel-slag-powder replacement ratios on the fracture properties (fracture energy, fracture toughness, and double-K fracture parameters) of the SPC were analyzed and discussed. The results showed that the incorporation of appropriate steel-slag powder can affect the fracture performance of SPC. Compared with concrete without steel-slag powder, adding appropriate steel-slag powder can effectively improve the bond performance between aggregate and matrix because the steel-slag powder contains hydration activity substances such as calcium oxide and aluminium trioxide. The fracture energy and fracture toughness of SPC increased and then decreased with the increase in steel-slag-powder replacement ratios, and the SPC concrete showed best fracture performance with a 5% steel slag powder replacement ratio. Its fracture energy increases by 13.63% and fracture toughness increases by 53.22% compared with NC.
Magnetic Resonance Studies of Hybrid Nanocomposites Containing Nanocrystalline TiO2 and Graphene-Related Materials
Authors: Guskos Nikos , Zolnierkiewicz Grzegorz , Kusiak-Nejman Ewelina , Guskos Aleksander , Aidinis Konstantinos , Bobrowska Marta , Berczynski Paweł , Wanag Agnieszka , Pelech Iwona , Narkiewicz Urszula , Morawski Antoni W.
Published: March 2022

Nanocomposites based on nanocrystalline titania modified with graphene-related materials (reduced and oxidized form of graphene) showed the existence of magnetic agglomerates. All parameters of magnetic resonance spectra strongly depended on the materials’ modification processes. The reduction of graphene oxide significantly increased the number of magnetic moments, which caused crucial changes in the reorientation and relaxation processes. At room temperature, a wide resonance line dominated for all nanocomposites studied and in some cases, a narrow resonance line derived from the conduction electrons. Some nanocomposites (samples of titania modified with graphene oxide, prepared with the addition of water or butan-1-ol) showed a single domain magnetic (ferromagnetic) arrangement, and others (samples of titania modified with reduced graphene oxide) exhibited magnetic anisotropy. In addition, the spectra of EPR from free radicals were observed for all samples at the temperature of 4 K. The magnetic resonance imaging methods enable the capturing of even a small number of localized magnetic moments, which significantly affects the physicochemical properties of the materials.
An Experimental Investigation of Static Properties of Bio-Oils and SAE40 Oil in Journal Bearing Applications
Authors: Sadiq Muhammad Imran , Ghopa Wan Aizon , Nuawi Mohd Zaki , Rasani Mohammad Rasidi , Ibrahim Sofian
Published: March 2022

Considerable research has been conducted in the past decade and a half regarding the bio-lubricants potential to replace mineral-based lubricants as mainstream lubricants such as engine oil, hydraulic oil, compressor oil, and metalworking oil. This study studied several bio-lubricants (rapeseed oil, palm olein, and soybean oil) and a mineral-based lubricant, SAE40. The bio-lubricants have better physiochemical, tribological characteristics and environmental friendly nature, and are promising to replace mineral-based lubricants. In this study, a journal bearing test rig (JBTR) was developed in order to investigate the effect of journal speed on the temperature of oil film with time. Additionally, the load-carrying capacity of bio-oils was tested against the mineral-based lubricant SAE40 by adding a load on the journal. For all three speeds, i.e., 1000, 1500, and 2000 rpm, the bio-lubricants recorded minimum temperature. At 1000 rpm, rapeseed oil recorded a 9.2% lower temperature than SAE40. Similarly, at 2000 rpm, rapeseed oil recorded a minimum temperature that was 2.5% lower than SAE40; in comparison, at 1500 rpm, palm olein recorded a minimum temperature that was 1.8% less than SAE40. Overall, the results of this study revealed that bio-oils recorded a lower temperature rise than mineral oil. These results are very encouraging for further research in this area.
Softened Microstructure and Properties of 12 μm Thick Rolled Copper Foil
Authors: Feng Rui , Zhao Weichao , Sun Yumei , Wang Xiaowen , Gong Benkui , Chang Baoping , Feng Tianjie
Published: March 2022

Up to now, 12 μm thick rolled copper foil is the thinnest rolled copper foil that can be stably produced. The softened microstructure and properties of 12 μm thick rolled copper foil were systematically studied in this paper. The softened process consists of thermal treatment at 180 °C for different times. The results show that the softened annealing texture is mainly cubic texture, and the cubic texture fraction increases with the increase in annealing time. The cubic texture fraction reaches the highest (34.4%) after annealing for 60 min. After annealing for 1–5 min, the tensile strength and the bending times decrease significantly. After annealing for 10–60 min, the tensile strength tends to be stable, and the bending times increase slightly. With the increase in annealing time, the electrical conductivity increases gradually, reaching 92% International Annealed Copper Standard (IACS) after annealing for 60 min. Electrical conductivity can be used as a fast and effective method to analyze the microstructure of metals.
Evaluation of Design Procedure and Performance of Continuously Reinforced Concrete Pavement According to AASHTO Design Methods
Authors: Moharekpour Milad , Liu Pengfei , Schmidt Joshua , Oeser Markus , Jing Ruxin
Published: March 2022

The Guide for Design of Pavement Structures (AASHTO 86/93) and Mechanistic Empirical Pavement Design Guide (MEPDG) are two common methods to design continuously reinforced concrete pavement (CRCP) published by the American Association of State Highway and Transportation Officials (AASHTO) in the USA. The AASHTO 86/93 is based on empirical equations to assess the performance of highway pavements under moving loads with known magnitude and frequency derived from experiments on AASHTO road tests. The MEPDG is a pavement design method based on engineering mechanics and numerical models for analysis. It functions by incorporating additional attributes such as environment, material properties, and vehicle axle load to predict pavement performance and degradation at the selected reliability level over the intended performance period. In order to evaluate the CRCP design procedure and performance, crack width (CW) and crack spacing (CS) from five examined test tracks in Europe with different climate condition, base layer, geometry, and materials were collected in this paper and compared with predicted distresses as well as CW and CS from AASHTO 86/93 and MEPDG design methods. The results show that the interactions between geometrics, material properties, traffic, and environmental conditions in the MEPDG method are more pronounced than in the AASHTO 86/93 and the prediction of CS and CW based on MEPDG matched closely with the recorded data from sections.
Influence of Pulse Duration on X-ray Emission during Industrial Ultrafast Laser Processing
Authors: Holland Julian , Weber Rudolf , Sailer Marc , Graf Thomas
Published: March 2022

Soft X-ray emissions during the processing of industrial materials with ultrafast lasers are of major interest, especially against the background of legal regulations. Potentially hazardous soft X-rays, with photon energies of >5 keV, originate from the fraction of hot electrons in plasma, the temperature of which depends on laser irradiance. The interaction of a laser with the plasma intensifies with growing plasma expansion during the laser pulse, and the fraction of hot electrons is therefore enhanced with increasing pulse duration. Hence, pulse duration is one of the dominant laser parameters that determines the soft X-ray emission. An existing analytical model, in which the fraction of hot electrons was treated as a constant, was therefore extended to include the influence of the duration of laser pulses on the fraction of hot electrons in the generated plasma. This extended model was validated with measurements of H (0.07) dose rates as a function of the pulse duration for a constant irradiance of about 3.5 × 1014 W/cm2, a laser wavelength of 800 nm, and a pulse repetition rate of 1 kHz, as well as for varying irradiance at the laser wavelength of 1030 nm and pulse repetition rates of 50 kHz and 200 kHz. The experimental data clearly verified the predictions of the model and confirmed that significantly decreased dose rates are generated with a decreasing pulse duration when the irradiance is kept constant.
Cellulose Nanofibrils as a Damping Material for the Production of Highly Crystalline Nanosized Zeolite Y via Ball Milling
Authors: Nassrullah Haya , Anis Shaheen Fatima , Lalia Boor Singh , Hashaikeh Raed
Published: March 2022

Nanosized zeolite Y is used in various applications from catalysis in petroleum refining to nanofillers in water treatment membranes. Ball milling is a potential and fast technique to decrease the particle size of zeolite Y to the nano range. However, this technique is associated with a significant loss of crystallinity. Therefore, in this study, we investigate the effect of adding biodegradable and recyclable cellulose nanofibrils (CNFs) to zeolite Y in a wet ball milling approach. CNFs are added to shield the zeolite Y particles from harsh milling conditions due to their high surface area, mechanical strength, and water gel-like format. Different zeolite Y to CNFs ratios were studied and compared to optimize the ball milling process. The results showed that the optimal zeolite Y to CNFs ratio is 1:1 to produce a median particle size diameter of 100 nm and crystallinity index of 32%. The size reduction process provided accessibility to the zeolite pores and as a result increased their adsorption capacity. The adsorption capacity of ball-milled particles for methylene blue increased to 29.26 mg/g compared to 10.66 mg/g of the pristine Zeolite. These results demonstrate the potential of using CNF in protecting zeolite Y particles and possibly other micro particles during ball milling.