bluuebunny/crossref_metadata_2025 huggingface dataset:
A Spatio-Temporal Analysis of the Frequency of Droughts in Mexico’s Forest Ecosystems
Authors: López-Teloxa Leticia Citlaly , Monterroso-Rivas Alejandro Ismael
Published: July 2024

Droughts can affect forest ecosystems and lead to soil degradation, biodiversity loss, and desertification. Not all regions of Mexico are affected in the same way, as some areas are naturally more prone to drought due to their geographical location. Therefore, the objective of this work was to carry out a spatio-temporal analysis of the occurrence of droughts (severe and extreme) in Mexican forest systems, covering the period 2000–2021, and to study the area covered by these events in Mexican forest systems. This analysis was divided into three stages: the classification of land use and vegetation, spatial mapping and the classification of drought intensity, and an analysis of drought frequency and probability in forest systems. The results show that more than 46% of Mexico’s forest area experienced severe and extreme droughts during the 21-year period studied. Broadleaved forests were most affected by severe and extreme droughts, with a frequency of 6 years. The increasing frequency of droughts poses a major challenge to the resilience of forest ecosystems in Mexico, highlighting the need to implement climate change adaptation and forest management measures to protect the country’s biodiversity and natural resources.
Biomass and Carbon Stock Capacity of Robinia pseudoacacia Plantations at Different Densities on the Loess Plateau
Authors: Hu Yawei , Zhao Jiongchang , Li Yang , Tang Peng , Yang Zhou , Zhang Jianjun , Sun Ruoxiu
Published: July 2024

Forests make an important contribution to the global carbon cycle and climate regulation. Caijiachuan watershed false acacia (Robinia pseudoacacia Linn.) plantation forests have been created for 30 years, but a series of problems have arisen due to the irrationality of the density involved at that time. To precisely assess the contribution of R. pseudoacacia plantations with different densities to this cycle, we measured the diameter at breast height (DBH), tree height (H), biomass, and carbon stocks in trees, shrubs, herbs, litter, and soil across different density ranges, denoted as D1 = 900–1400, D2 = 1401–1900, D3 = 1901–2400, D4 = 2401–2900, and D5 = 2901–3400 trees ha−1. In order to achieve the purpose of accurately estimating the biomass, carbon stocks and the contribution rate of each part in different densities of R. pseudoacacia plantations were measured. The results are as follows: (1) Both DBH and H decreased with increasing density, and field surveys were much more difficult and less accurate for H than DBH. Based on the two allometric growth models, it was found that the determination coefficient of the biomass model that incorporated both H and DBH (0.90) closely resembled that of the model using only DBH (0.89), with an error margin of only 0.04%. (2) At the sample scale, stand density significantly affected R. pseudoacacia stem biomass and total biomass. At the individual plant scale, stand density significantly affected R. pseudoacacia organ biomass. Increasing stand densities promoted the accumulation of vegetation biomass within the sample plot but did not improve the growth of individual R. pseudoacacia trees. The stem biomass constituted the majority of the total R. pseudoacacia biomass (58.25%–60.62%); the total R. pseudoacacia biomass represented a significant portion of the vegetation biomass (93.02%–97.37%). (3) The total carbon stock in the sample plots tended to increase with increasing stand density, indicating a positive correlation between density and the carbon stock of the whole plantation forest ecosystem. Hence, in future R. pseudoacacia plantations, appropriate densities should be selected based on specific objectives. For wood utilization, a planting density of 900–1400 trees ha−1 should be controlled. For carbon fixation, an initial planting density of 2900–3400 trees ha−1 should be selected for R. pseudoacacia. This study provides theoretical support for local forest management and how to better sequester carbon.
A Comparative Literature Review of Machine Learning and Image Processing Techniques Used for Scaling and Grading of Wood Logs
Authors: Sandvik Yohann Jacob , Futsæther Cecilia Marie , Liland Kristian Hovde , Tomic Oliver
Published: July 2024

This literature review assesses the efficacy of image-processing techniques and machine-learning models in computer vision for wood log grading and scaling. Four searches were conducted in four scientific databases, yielding a total of 1288 results, which were narrowed down to 33 relevant studies. The studies were categorized according to their goals, including log end grading, log side grading, individual log scaling, log pile scaling, and log segmentation. The studies were compared based on the input used, choice of model, model performance, and level of autonomy. This review found a preference for images over point cloud representations for logs and an increase in camera use over laser scanners. It identified three primary model types: classical image-processing algorithms, deep learning models, and other machine learning models. However, comparing performance across studies proved challenging due to varying goals and metrics. Deep learning models showed better performance in the log pile scaling and log segmentation goal categories. Cameras were found to have become more popular over time compared to laser scanners, possibly due to stereovision cameras taking over for laser scanners for sampling point cloud datasets. Classical image-processing algorithms were consistently used, deep learning models gained prominence in 2018, and other machine learning models were used in studies published between 2010 and 2018.
Impact of Conservation in the Futian Mangrove National Nature Reserve on Water Quality in the Last Twenty Years
Authors: Luo Jin , Huang Qiming , Zhang Hongsheng , Xu Yanhua , Zu Xiaofang , Song Bin
Published: July 2024

Mangroves play a crucial role in improving the water quality of mangrove wetlands. However, current research faces challenges, such as the difficulty in quantifying the impact of mangroves on water quality and the unclear pathways of influence. This study utilized remote sensing imagery to investigate the long-term changes in mangrove forests in the Futian Mangrove National Nature Reserve and constructed a water quality index based on water quality data. Finally, structural equation modeling was employed to explore the pathways of influence and quantify the impact effects of mangroves, climate, and water quality. The study findings revealed several key points: (1) The mangrove forests in the Futian Mangrove National Nature Reserve exhibited a trend of expansion towards the ocean during this period. (2) The seasonal and annual characteristics of water quality in Shenzhen Bay indicated a significant improvement in water quality from 2000 to 2020. (3) Mangroves have significant direct and indirect impacts on water quality, which are more pronounced than the effects of climate factors. These findings not only offer insights for the environmental management and conservation of Shenzhen Bay but also provide support for future comprehensive studies on the response relationships between the morphology, species, and physiological characteristics of mangroves and water quality.
Effect of Mixed Cultures on Microbiological Development in Berliner Weisse Beer
Authors: Hübbe Thomas , Reitenbach Amanda Felipe , Burin Vívian Maria , Ghesti Grace Ferreira , Jürgen Frank
Published: July 2024

Sour beers play an important role in the brewing market, and their production has been growing exponentially. In light of this, six microorganisms directly related to this class of beer were studied, and the fermentation behavior of six strains used in the past for traditional commercial Berliner Weisse beer production was monitored. The microorganisms used were Lactobacillus brevis, Lactobacillus parabrevis, Brettanomyces bruxellensis, and Brettanomyces anomalus and two strains of Saccharomyces cerevisiae. The six microorganisms were selected in a previous work, and a comparison between single and mixed fermentations was carried out via daily measurements of the fermentation parameters like pH, extract, and cell count during 22 days. The ability to isolate a specific microorganism from a mixed culture was investigated using three commonly used nutrient media and aerobic/anaerobic growth conditions. Both Lactobacillus and Brettanomyces could be isolated; however, the conditions imposed were not sufficient in order to isolate Saccharomyces. Fermentations carried out with LAB and Brettanomyces showed a decrease in Lactobacillus growth if compared to pure fermentations, but no influence on the growth of Brettanomyces could be perceived. In general, fermentations carried out in the presence of Saccharomyces were dominated by this yeast. Its quick growth seems to be responsible for the high end pH values observed as well as the decrease in cell growth for both LAB and Brettanomyces. A decrease in the cell viability of Saccharomyces was followed by an increased growth of the other microorganisms involved, possibly meaning that the molecules released through apoptosis are used by both LAB and Brettanomyces as a valuable nutrient source. The volatile compound concentrations of the first group were higher in fermentations with Saccharomyces, whereas esters’ concentration was higher in fermentations carried out only with Brettanomyces and Lactobacillus. Furthermore, understanding how these microorganisms interact during the fermentation process can help brewers better control production and ensure the consistency in the quality of the final product. The end pH values and acidity reached levels acceptable for Berliner Weisse beer. This innovative approach certainly contributes to the evolution and refinement of the art of brewing.
NFT Technology for Enhanced Global Digital Registers: A Novel Approach to Tokenization
Authors: Kuznetsov Oleksandr , Frontoni Emanuele , Kuznetsova Kateryna , Shevchuk Ruslan , Karpinski Mikolaj
Published: July 2024

In the rapidly evolving field of digital asset management, centralized and decentralized global registries have become essential tools for organizing, tracking, and distributing digital assets. However, existing systems often face challenges regarding security, censorship resistance, interoperability, customizability, and scalability. This research paper aims to address these gaps by proposing a novel decentralized global registry system based on blockchain technology and non-fungible tokens (NFTs). The research paper makes several key contributions to the field of digital asset management. First, it provides a detailed system design for the proposed decentralized global registry, outlining its architectural components, functional modules, and integration with blockchain and NFT technologies. Second, it offers a thorough comparative analysis of the advantages and limitations of the proposed system in relation to existing centralized and decentralized registries. Finally, the paper presents potential use cases and practical applications of the proposed system in various industries, demonstrating its versatility and adaptability to different contexts and requirements. In conclusion, this research paper contributes significantly to the ongoing efforts to improve digital asset management by presenting a novel, decentralized global registry system based on blockchain technology and NFTs. The proposed system addresses the key limitations of existing solutions and offers a promising direction for future research and development in this critical field.
A Comparative Study of Fire Code Classifications of Building Materials
Authors: Sadaoui Amirouche , Dagenais Christian , Blanchet Pierre
Published: July 2024

Whether noncombustible or combustible construction is used, the presence of combustible materials is likely to be used for various reasons, such as interior finishes, flooring, and insulation. Consequently, how regulations consider the degree of combustibility in their fire classifications will influence the level of fire safety provided in these buildings and the exchanges between all actors in the construction sector. In North America, the regulation of combustibility is primarily governed by surface flame spread assessed through the Steiner tunnel test. While there is a growing prevalence of calorimetric methods globally, their incorporation into North American building codes remains notably limited. Based on ISO 5660-1 cone calorimeter test results of twenty commercially available North American building materials, a comparative study was conducted between the Canadian flame spread classification and the classifications in Japan, New Zealand and the European Union (Euroclass). The tests and their limitations are described herein, as well as the conceptual frameworks. The results suggest that as materials’ combustibility levels increase, the level of agreement between classifications decreases and remains binary. The choice between the material and system scales is crucial for determining the effective development and implementation of regulations.
Expression of Concern: Starkute et al. Characteristics of Unripened Cow Milk Curd Cheese Enriched with Raspberry (Rubus idaeus), Blueberry (Vaccinium myrtillus) and Elderberry (Sambucus nigra) Industry By-Products. Foods 2023, 12, 2860
Authors:   Foods Editorial Office
Published: July 2024

With this notice, the Foods Editorial Office alerts readers to concerns related to this article [...]
Ratanjot (Alkanna tinctoria L.) Root Extract, Rich in Antioxidants, Exhibits Strong Antimicrobial Activity against Foodborne Pathogens and Is a Potential Food Preservative
Authors: Das Annada , Biswas Subhasish , Satyaprakash Kaushik , Bhattacharya Dipanwita , Nanda Pramod Kumar , Patra Gopal , Moirangthem Sushmita , Nath Santanu , Dhar Pubali , Verma Arun K. , Biswas Olipriya , Tardi Nicole Irizarry , Bhunia Arun K. , Das Arun K.
Published: July 2024

Natural and sustainable plant-based antioxidants and antimicrobials are highly desirable for improving food quality and safety. The present investigation assessed the antimicrobial and antioxidant properties of active components from Alkanna tinctoria L. (herb) roots, also known as Ratanjot root. Two methods were used to extract active components: microwave-assisted hot water (MAHW) and ethanolic extraction. MAHW extract yielded 6.29%, while the ethanol extract yielded 18.27%, suggesting superior Ratanjot root extract powder (RRP) solubility in ethanol over water. The ethanol extract showed significantly higher antioxidant activity than the MAHW extract. Gas Chromatography–Mass Spectrometry analysis revealed three major phenolic compounds: butanoic acid, 3-hydroxy-3-methyl-; arnebin 7, and diisooctyl pthalate. The color attributes (L*, a*, b*, H°ab, C*ab) for the ethanolic and MAHW extracts revealed significant differences (p < 0.05) in all the above parameters for both types of extracts, except for yellowness (b*) and chroma (C*ab) values. The ethanol extract exhibited antimicrobial activity against 14 foodborne bacteria, with a significantly higher inhibitory effect against Gram-positive bacteria (Listeria monocytogenes and Staphylococcus aureus) than the Gram-negative bacteria (Salmonella enterica serovar Typhimurium and Escherichia coli). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both 25 mg/mL for the Gram-negative bacteria, while the MIC and MBC concentrations varied for Gram-positive bacteria (0.049–0.098 mg/mL and 0.098–0.195 mg/mL) and the antimicrobial effect was bactericidal. The antimicrobial activities of RRP extract remained stable under broad temperature (37–100 °C) and pH (2–6) conditions, as well as during refrigerated storage for 30 days. Application of RRP at 1% (10 mg/g) and 2.5% (25 mg/g) levels in a cooked chicken meatball model system prevented lipid oxidation and improved sensory attributes and retarded microbial growth during refrigerated (4 °C) storage for 20 days. Furthermore, the RRP extract was non-toxic when tested with sheep erythrocytes and did not inhibit the growth of probiotics, Lacticaseibacillus casei, and Lactiplantibacillus plantarum. In conclusion, the study suggests that RRP possesses excellent antimicrobial and antioxidant activities, thus making it suitable for food preservation.
Functional Nanostructured Lipid Carrier-Enriched Hydrogels Tailored to Repair Damaged Epidermal Barrier
Authors: Joukhadar Radwan , Nižić Nodilo Laura , Lovrić Jasmina , Hafner Anita , Pepić Ivan , Jug Mario
Published: July 2024

In this study, a functional nanostructured lipid carriers (NLCs)-based hydrogel was developed to repair the damaged epidermal skin barrier. NLCs were prepared via a high-energy approach, using argan oil and beeswax as liquid and solid lipids, respectively, and were loaded with ceramides and cholesterol at a physiologically relevant ratio, acting as structural and functional compounds. Employing a series of surfactants and optimizing the preparation conditions, NLCs of 215.5 ± 0.9 nm in size and a negative zeta potential of −42.7 ± 0.9 were obtained, showing acceptable physical and microbial stability. Solid state characterization by differential scanning calorimetry and X-ray powder diffraction revealed the formation of imperfect crystal NLC-type. The optimized NLC dispersion was loaded into the gel based on sodium hyaluronate and xanthan gum. The gels obtained presented a shear thinning and thixotropic behavior, which is suitable for dermal application. Incorporating NLCs enhanced the rheological, viscoelastic, and textural properties of the gel formed while retaining the suitable spreadability required for comfortable application and patient compliance. The NLC-loaded gel presented a noticeable occlusion effect in vitro. It provided 2.8-fold higher skin hydration levels on the ex vivo porcine ear model than the NLC-free gel, showing a potential to repair the damaged epidermal barrier and nourish the skin actively.
Incorporation of Locust Bean Gum and Solid Lipid Microparticles as Strategies to Improve the Properties and Stability of Calcium-Rich Soy Protein Isolate Gels
Authors: Brito-Oliveira Thais C. , Cavini Ana Clara M. , Ferreira Leticia S. , Moraes Izabel C. F. , Pinho Samantha C.
Published: July 2024

The present study aimed to investigate the properties of calcium-rich soy protein isolate (SPI) gels (14% SPI; 100 mM CaCl2), the effects of incorporating different concentrations locust bean gum (LBG) (0.1–0.3%, w/v) to the systems and the stability of the obtained gels. Also, the incorporation of solid lipid microparticles (SLMs) was tested as an alternative strategy to improve the system’s stability and, therefore, potential to be applied as a product prototype. The gels were evaluated regarding their visual aspect, rheological properties, water-holding capacities (WHCs) and microstructural organizations. The CaCl2-induced gels were self-supported but presented low WHC (40.0% ± 2.2) which was improved by LBG incorporation. The obtained mixed system, however, presented low stability, with high syneresis after 10 days of storage, due to microstructural compaction. The gels’ stability was improved by SLM incorporation, which decreased the gelled matrices’ compaction and syneresis for more than 20 days. Even though the rheological properties of the emulsion-filled gels (EFGs) were very altered due to the ageing process (which may affect the sensory perception of a future food originated from this EFG), the incorporation of SLMs increased the systems potential to be applied as a calcium-rich product prototype.
An Overview of N2O Emissions from Cropping Systems and Current Strategies to Improve Nitrogen Use Efficiency
Authors: Manco Antonio , Giaccone Matteo , Zenone Terenzio , Onofri Andrea , Tei Francesco , Farneselli Michela , Gabbrielli Mara , Allegrezza Marina , Perego Alessia , Magliulo Vincenzo , Vitale Luca
Published: July 2024

Arable soils significantly contribute to atmosphere pollution through N2O emissions due to the massive use of N-based fertilizers and soil managements. N2O formation in the soil occurs mainly through nitrification and denitrification processes, which are influenced by soil moisture, temperature, oxygen concentration, pH, and the amount of available organic carbon and nitrogen. This review synthetically presents the mechanisms of N2O formation and emission in arable land and some of the current strategies to improve crop nutrient use efficiency. Biological nitrification inhibitor-based agronomic strategies are also presented as future prospects for the sustainable management of crops, which is missing in most of the reviews.
Bibliometric Analysis on the Research of Geoscience Knowledge Graph (GeoKG) from 2012 to 2023
Authors: Hou Zhi-Wei , Liu Xulong , Zhou Shengnan , Jing Wenlong , Yang Ji
Published: July 2024

The geoscience knowledge graph (GeoKG) has gained worldwide attention due to its ability in the formal representation of spatiotemporal features and relationships of geoscience knowledge. Currently, a quantitative review of the state and trends in GeoKG is still scarce. Thus, a bibliometric analysis was performed in this study to fill the gap. Specifically, based on 294 research articles published from 2012 to 2023, we conducted analyses in terms of the (1) trends in publications and citations; (2) identification of the major papers, sources, researchers, institutions, and countries; (3) scientific collaboration analysis; and (4) detection of major research topics and tendencies. The results revealed that the interest in GeoKG research has rapidly increased after 2019 and is continually expanding. China is the most productive country in this field. Co-authorship analysis shows that inter-national and inter-institutional collaboration should be reinforced. Keyword analysis indicated that geoscience knowledge representation, information extraction, GeoKG construction, and GeoKG-based multi-source data integration were current hotspots. In addition, several important but currently neglected issues, such as the integration of Large Language Models, are highlighted. The findings of this review provide a systematic overview of the development of GeoKG and provide a valuable reference for future research.
Differential Expression of Circulating miRNAs and Carfilzomib-Related Cardiovascular Adverse Events in Patients with Multiple Myeloma
Authors: Tantawy Marwa , Langaee Taimour , Wang Danxin , Rubinstein Samuel M. , Cornell Robert F. , Lenihan Daniel , Fradley Michael G. , Gong Yan
Published: July 2024

This study investigates the association between circulating microRNA (miRNA) expression and cardiovascular adverse events (CVAE) in multiple myeloma (MM) patients treated with a carfilzomib (CFZ)-based regimen. A cohort of 60 MM patients from the Prospective Observation of Cardiac Safety with Proteasome Inhibitor (PROTECT) study was analyzed. Among these, 31 patients (51.6%) developed CVAE post-CFZ treatment. The Taqman OpenArray Human microRNA panels were used for miRNA profiling. We identified 13 differentially expressed miRNAs at baseline, with higher expressions of miR-125a-5p, miR-15a-5p, miR-18a-3p, and miR-152-3p and lower expression of miR-140-3p in patients who later developed CVAE compared to those free of CVAE, adjusting for age, gender, race, and higher B-type natriuretic peptide levels. We also identified three miRNAs, including miR-150-5p, that were differentially expressed in patients with and without CVAE post-treatment. Additionally, five miRNAs responded differently to CFZ treatment in CVAE vs. non-CVAE patients, including significantly elevated post-treatment expression of miR-140-3p and lower expressions of miR-598, miR-152, miR-21, and miR-323a in CVAE patients. Pathway enrichment analysis highlighted the involvement of these miRNAs in cardiovascular diseases and vascular processes. These findings suggest that specific miRNAs could serve as predictive biomarkers for CVAE and provide insights into the underlying mechanisms of CFZ-CVAE. Further investigation is warranted before these findings can be applied in clinical settings.
Following the Trace of Cyclodextrins on the Selenium and Tellurium Odyssey
Authors: Morán-Serradilla Cristina , Plano Daniel , Sharma Arun K. , Sanmartín Carmen
Published: July 2024

There is an urgent need to develop safer and more effective modalities for the treatment of numerous pathologies due to the increasing rates of drug resistance, undesired side effects, poor clinical outcomes, etc. Over the past decades, cyclodextrins (CDs) have gathered great attention as potential drug carriers due to their ability to enhance their bioactivities and properties. Likewise, selenium (Se) and tellurium (Te) have been extensively studied during the last decades due to their possible therapeutical applications. Although there is limited research on the relationship between Se and Te and CDs, herein, we highlight different representative examples of the advances related to this topic as well as give our view on the future directions of this emerging area of research. This review encompasses three different aspects of this relationship: (1) modification of the structure of the different CDs; (2) formation of host–guest interaction complexes of naïve CDs with Se and Te derivatives in order to overcome specific limitations of the latter; and (3) the use of CDs as catalysts to achieve novel Se and Te compounds.
Potential Consequences of the Use of Adipose-Derived Stem Cells in the Treatment of Hepatocellular Carcinoma
Authors: Gładyś Aleksandra , Mazurski Adam , Czekaj Piotr
Published: July 2024

Hepatocellular carcinoma (HCC) ranks as the most prevalent of primary liver cancers and stands as the third leading cause of cancer-related deaths. Early-stage HCC can be effectively managed with available treatment modalities ranging from invasive techniques, such as liver resection and thermoablation, to systemic therapies primarily employing tyrosine kinase inhibitors. Unfortunately, these interventions take a significant toll on the body, either through physical trauma or the adverse effects of pharmacotherapy. Consequently, there is an understandable drive to develop novel HCC therapies. Adipose-derived stem cells (ADSCs) are a promising therapeutic tool. Their facile extraction process, coupled with the distinctive immunomodulatory capabilities of their secretome, make them an intriguing subject for investigation in both oncology and regenerative medicine. The factors they produce are both enzymes affecting the extracellular matrix (specifically, metalloproteinases and their inhibitors) as well as cytokines and growth factors affecting cell proliferation and invasiveness. So far, the interactions observed with various cancer cell types have not led to clear conclusions. The evidence shows both inhibitory and stimulatory effects on tumor growth. Notably, these effects appear to be dependent on the tumor type, prompting speculation regarding their potential inhibitory impact on HCC. This review briefly synthesizes findings from preclinical and clinical studies examining the effects of ADSCs on cancers, with a specific focus on HCC, and emphasizes the need for further research.
Establishing Normal Serum Values of Neurofilament Light Chains and Glial Fibrillary Acidic Protein Considering the Effects of Age and Other Demographic Factors in Healthy Adults
Authors: Rodero-Romero Alexander , Monreal Enric , Sainz-Amo Raquel , García Domínguez José Manuel , Villarrubia Noelia , Veiga-González Jose Luís , Fernández-Velasco José Ignacio , Goicochea-Briceño Haydee , Rodríguez-Jorge Fernando , Sainz de la Maza Susana , Chico-García Juan Luís , Muriel Alfonso , Masjuan Jaime , Costa-Frossard Lucienne , Villar Luisa María
Published: July 2024

Multiple studies have shown the importance of blood-based biomarkers indicating axonal damage (serum neurofilament light chains [sNfL]) or astroglia activation (serum glial fibrillary acidic protein [sGFAP]) for monitoring different neurological diseases. However, normal values of these variables remain to be clearly defined, partly due to the influence of different demographic factors. We investigated demographic differences in a cohort of healthy volunteers. A cross-sectional study was conducted including 116 healthy controls with ages between 18 and 69 years (67.5% females; n = 79). sNfL and sGFAP concentrations were measured using single-molecule arrays. Age and body mass index affected sNfL values, and age was found to be the most important factor. The normal values changed with age, and we established normal values for individuals younger than 45 years as <10 pg/mL and for controls older than 45 years as <15 pg/mL. We established normal values at <10 pg/mL for individuals younger than 45 years and <15 pg/mL for older individuals. Alternatively, a Z-score of 1.5 was relevant for all controls. sGFAP was only affected by age. Differences in normal values were evident by 55 years. The highest normality limit for sGFAP was 140 pg/mL for controls under 55 years and 280 for older controls. We defined normal levels for sNfL and sGFAP and their corresponding age-associated changes. These data may contribute to the application of such variables in clinical practice.
Fabrication and Biomedical Application of Alginate Composite Hydrogels in Bone Tissue Engineering: A Review
Authors: Chen Xiuqiong , Wu Ting , Bu Yanan , Yan Huiqiong , Lin Qiang
Published: July 2024

Nowadays, as a result of the frequent occurrence of accidental injuries and traumas such as bone damage, the number of people causing bone injuries or fractures is increasing around the world. The design and fabrication of ideal bone tissue engineering (BTE) materials have become a research hotspot in the scientific community, and thus provide a novel path for the treatment of bone diseases. Among the materials used to construct scaffolds in BTE, including metals, bioceramics, bioglasses, biomacromolecules, synthetic organic polymers, etc., natural biopolymers have more advantages against them because they can interact with cells well, causing natural polymers to be widely studied and applied in the field of BTE. In particular, alginate has the advantages of excellent biocompatibility, good biodegradability, non-immunogenicity, non-toxicity, wide sources, low price, and easy gelation, enabling itself to be widely used as a biomaterial. However, pure alginate hydrogel as a BTE scaffold material still has many shortcomings, such as insufficient mechanical properties, easy disintegration of materials in physiological environments, and lack of cell-specific recognition sites, which severely limits its clinical application in BTE. In order to overcome the defects of single alginate hydrogels, researchers prepared alginate composite hydrogels by adding one or more materials to the alginate matrix in a certain proportion to improve their bioapplicability. For this reason, this review will introduce in detail the methods for constructing alginate composite hydrogels, including alginate/polymer composite hydrogels, alginate/bioprotein or polypeptide composite hydrogels, alginate/bioceramic composite hydrogels, alginate/bioceramic composite hydrogels, and alginate/nanoclay composite hydrogels, as well as their biological application trends in BTE scaffold materials, and look forward to their future research direction. These alginate composite hydrogel scaffolds exhibit both unexceptionable mechanical and biochemical properties, which exhibit their high application value in bone tissue repair and regeneration, thus providing a theoretical basis for the development and sustainable application of alginate-based functional biomedical materials.
Plant Hormone Pathway Is Involved in Regulating the Embryo Development Mechanism of the Hydrangea macrophylla Hybrid
Authors: Zhu Yali , Zeng Xiaoman , Zhu Tingting , Jiang Hui , Lei Penghu , Zhang Huijun , Chen Haixia
Published: July 2024

The research is aimed to elucidate the role of plant hormones in regulating the development of hybrid embryos in Hydrangea macrophylla. Fruits from the intraspecific cross of H. macrophylla ‘Otaksa’ × ‘Coerulea’ were selected at the globular, heart, and torpedo stages of embryo development. Transcriptome sequencing and differential gene expression analysis were conducted. The results showed that fruit growth followed a single “S-shaped growth curve, with globular, heart, and torpedo embryos appearing at 30, 40, and 50 d post-pollination, respectively, and the embryo maintaining the torpedo shape from 60 to 90 d. A total of 12,933 genes was quantified across the three developmental stages, with 3359, 3803, and 3106 DEGs in the S1_vs_S2, S1_vs_S3, and S2_vs_S3 comparisons, respectively. Among these, 133 genes related to plant hormone biosynthesis and metabolism were differentially expressed, regulating the synthesis and metabolism of eight types of plant hormones, including cytokinin, auxin, gibberellin, abscisic acid, and jasmonic acid. The pathways with the most differentially expressed genes were cytokinin, auxin, and gibberellin, suggesting these hormones may play crucial roles in embryo development. In the cytokinin pathway, CKX (Hma1.2p1_0579F.1_g182670.gene, Hma1.2p1_1194F.1_g265700.gene, and NewGene_12164) genes were highly expressed during the globular embryo stage, promoting rapid cell division in the embryo. In the auxin pathway, YUC (Hma1.2p1_0271F.1_g109005.gene and Hma1.2p1_0271F.1_g109020.gene) genes were progressively up-regulated during embryo growth; the early response factor AUX/IAA (Hma1.2p1_0760F.1_g214260.gene) was down-regulated, while the later transcriptional activator ARF (NewGene_21460, NewGene_21461, and Hma1.2p1_0209F.1_g089090.gene) was up-regulated, sustaining auxin synthesis and possibly preventing the embryo from transitioning to maturity. In the gibberellin pathway, GA3ox (Hma1.2p1_0129F.1_g060100.gene) expression peaked during the heart embryo stage and then declined, while the negative regulator GA2ox (Hma1.2p1_0020F.1_g013915.gene) showed the opposite trend; and the gibberellin signaling repressor DELLA (Hma1.2p1_1054F.1_g252590.gene) increased over time, potentially inhibiting embryo development and maintaining the torpedo shape until fruit maturity. These findings preliminarily uncover the factors affecting the development of hybrid H. macrophylla embryos, laying a foundation for further research into the regulatory mechanisms of H. macrophylla hybrid embryo development.
Environmental and Genetic Determinants of Ankylosing Spondylitis
Authors: Bilski Rafał , Kamiński Piotr , Kupczyk Daria , Jeka Sławomir , Baszyński Jędrzej , Tkaczenko Halina , Kurhaluk Natalia
Published: July 2024

Exposure to heavy metals and lifestyle factors like smoking contribute to the production of free oxygen radicals. This fact, combined with a lowered total antioxidant status, can induce even more damage in the development of ankylosing spondylitis (AS). Despite the fact that some researchers are looking for more genetic factors underlying AS, most studies focus on polymorphisms within the genes encoding the human leukocyte antigen (HLA) system. The biggest challenge is finding the effective treatment of the disease. Genetic factors and the influence of oxidative stress, mineral metabolism disorders, microbiota, and tobacco smoking seem to be of great importance for the development of AS. The data contained in this review constitute valuable information and encourage the initiation and development of research in this area, showing connections between inflammatory disorders leading to the pathogenesis of AS and selected environmental and genetic factors.
Selenium Nanoparticle and Melatonin Treatments Improve Melon Seedling Growth by Regulating Carbohydrate and Polyamine
Authors: Kang Lu , Jia Yujiao , Wu Yangliu , Liu Hejiang , Zhao Duoyong , Ju Yanjun , Pan Canping , Mao Jiefei
Published: July 2024

Bio-stimulants, such as selenium nanoparticles and melatonin, regulate melon growth. However, the effects of individual and combined applications of selenium nanoparticles and melatonin on the growth of melon seedlings have not been reported. Here, two melon cultivars were sprayed with selenium nanoparticles, melatonin, and a combined treatment, and physiological and biochemical properties were analyzed. The independent applications of selenium nanoparticles, melatonin, and their combination had no significant effects on the plant heights and stem diameters of Jiashi and Huangmengcui melons. Compared with the controls, both selenium nanoparticle and melatonin treatments increased soluble sugars (6–63%) and sucrose (11–88%) levels, as well as the activity of sucrose phosphate synthase (171–237%) in melon leaves. The phenylalanine ammonia lyase (29–95%), trans cinnamate 4-hydroxylase (32–100%), and 4-coumaric acid CoA ligase (26–113%), as well as mRNA levels, also increased in the phenylpropanoid metabolism pathway. Combining the selenium nanoparticles and melatonin was more effective than either of the single treatments. In addition, the levels of superoxide dismutase (43–130%), catalase (14–43%), ascorbate peroxidase (44–79%), peroxidase (25–149%), and mRNA in melon leaves treated with combined selenium nanoparticles and melatonin were higher than in controls. The results contribute to our understanding of selenium nanoparticles and melatonin as bio-stimulants that improve the melon seedlings’ growth by regulating carbohydrate, polyamine, and antioxidant capacities.
Analyzing the Impact of the Highest Expressed Epstein–Barr Virus-Encoded microRNAs on the Host Cell Transcriptome
Authors: Hohmann Tim , Hohmann Urszula , Dehghani Faramarz , Grisk Olaf , Jasinski-Bergner Simon
Published: July 2024

The Epstein–Barr virus (EBV) has a very high prevalence (>90% in adults), establishes a lifelong latency after primary infection, and exerts an oncogenic potential. This dsDNA virus encodes for various molecules, including microRNAs (miRs), which can be detected in the latent and lytic phases with different expression levels and affect, among others, immune evasion and malignant transformation. In this study, the different EBV miRs are quantified in EBV-positive lymphomas, and the impact on the host cell transcriptome of the most abundant EBV miRs will be analyzed using comparative RNA sequencing analyses. The EBV miRs ebv-miR-BART1, -BART4, -BART17, and -BHRF1-1 were most highly expressed, and their selective overexpression in EBV-negative human cells resulted in a large number of statistically significantly down- and up-regulated host cell genes. Functional analyses showed that these dysregulated target genes are involved in important cellular processes, including growth factor pathways such as WNT, EGF, FGF, and PDGF, as well as cellular processes such as apoptosis regulation and inflammation. Individual differences were observed between these four analyzed EBV miRs. In particular, ebv-miR-BHRF1-1 appears to be more important for malignant transformation and immune evasion than the other EBV miRs.
Preconception Physical Exercise Is Associated with Phenotype-Specific Cardiovascular Alterations in Women at Risk for Gestational Hypertensive Disorders
Authors: Dreesen Pauline , Volders Pauline , Lanssens Dorien , Nouwen Sandy , Vrancken Birgit , Janssen Febe , Eijnde Bert O. , Hansen Dominique , Ceulemans Michael , Soubry Adelheid , Gyselaers Wilfried
Published: July 2024

Background/Objectives: Gestational hypertensive disorders (GHD) pose significant maternal and fetal health risks during pregnancy. Preconception physical exercise has been associated with a lower incidence of GHD, but insights into the cardiovascular mechanisms remain limited. This study aimed to evaluate the effect of preconception physical exercise on the complete cardiovascular functions of women at risk for GHD in a subsequent pregnancy. Methods: A non-invasive hemodynamics assessment of arteries, veins, and the heart was performed on 40 non-pregnant women at risk for developing GHD in a subsequent pregnancy. Measurements of an electrocardiogram Doppler ultrasound, impedance cardiography and bio-impedance spectrum analysis were taken before and after they engaged in physical exercise (30–50 min, 3×/week, 4–6 months). Results: After a mean physical exercise period of 29.80 weeks, the total peripheral resistance (TPR), diastolic blood pressure and mean arterial pressure decreased in the total study population, without changing cardiac output (CO). However, in 42% (9/21) of women categorized with high or low baseline CO (>P75 or <P25 resp.), a shift in CO was observed towards the normal reference interquartile range (P25–P75). This was associated with improved hepatic venous and central arterial hemodynamic functions. Similar changes in TPR occurred in 38% (11/29) of women classified as having low or high baseline TPR. Conclusions: As in pregnancy, output- or resistance-dominant cardiovascular profiles already exist prior to conception. This study illustrates that preconception physical exercise shifts high or low CO and/or TPR towards the normal midrange, allowing women at risk for GHD to start a subsequent pregnancy with a more gestation-adaptable cardiovascular system.
Patients’ Identification, Management and Prevention of Adverse Drug Reactions: A Cross-Sectional Survey of Patients with Severe Adverse Drug Reactions
Authors: Srisuriyachanchai Warisara , Cox Anthony R. , Jarernsiripornkul Narumol
Published: July 2024

Background: Few studies have been conducted on how patients identify, manage, and prevent severe adverse drug reactions (ADRs). This study aimed to explore the reasoning that patients use to identify symptoms of severe ADRs and the methods they employ to manage and prevent them. Methods: A cross-sectional survey using structured telephone interviews was administered to patients with a self-assessed severe ADR and to patients with serious skin ADRs from a hospital medical record database (in-patient and out-patient) from 1 September 2016 to 1 September 2019. Patients identified via the medical records were asked to assess their ADR for severity, and only patients that rated their ADR as severe were followed up with a telephone interview. Structured telephone interviews were conducted with respondents by a research pharmacist and audio-recorded. Results: A total of 722 patients with a severe ADR were identified, with 300 completing the interview (41.6%). The most frequently cited reasons for classifying ADRs as severe was worsening ADR symptoms (58.3%), severe ADR symptoms (44.4%), and ADR symptoms interfering with their life (36.4%). Only severe ADR symptoms were significantly different between the questionnaire and the medical records database groups (p = 0.007). The most frequent method of ADR management was discontinuation of drug by physicians (88.3%). About 79.0% of patients stated that they increased their carefulness when using other drugs after experiencing ADRs. The main method patients used to prevent ADRs was informing healthcare professionals (HCPs) about their drug allergy history (65.7%). Conclusions: Worsening ADR symptoms were often used to identify severe ADRs. However, HCPs were mainly responsible for the management and prevention of severe ADRs. Increasing awareness of ADRs by HCPs, and providing additional drug information, may improve patient safety.
A Comprehensive Geriatric Workup and Frailty Assessment in Older Patients with Severe Aortic Stenosis
Authors: Brunetti Enrico , Lucà Fabiana , Presta Roberto , Marchionni Niccolò , Boccanelli Alessandro , Ungar Andrea , Rao Carmelo Massimiliano , Ingianni Nadia , Lettino Maddalena , Del Sindaco Donatella , Murrone Adriano , Riccio Carmine , Colivicchi Furio , Grimaldi Massimo , Gulizia Michele Massimo , Oliva Fabrizio , Bo Mario , Parrini Iris
Published: July 2024

Aortic stenosis (AS) represents a notable paradigm for cardiovascular (CV) and geriatric disorders owing to comorbidity. Transcatheter aortic valve replacement (TAVR) was initially considered a therapeutic strategy in elderly individuals deemed unsuitable for or at high risk of surgical valve replacement. The progressive improvement in TAVR technology has led to the need to refine older patients’ stratification, progressively incorporating the concept of frailty and other geriatric vulnerabilities. Recognizing the intricate nature of the aging process, reliance exclusively on chronological age for stratification resulted in an initial but inadequate tool to assess both CV and non-CV risks effectively. A comprehensive geriatric evaluation should be performed before TAVR procedures, taking into account both physical and cognitive capabilities and post-procedural outcomes through a multidisciplinary framework. This review adopts a multidisciplinary perspective to delve into the diagnosis and holistic management of AS in elderly populations in order to facilitate decision-making, thereby optimizing outcomes centered around patient well-being.
An Umbrella Review of Body Image Concerns, Disordered Eating, and Eating Disorders in Elite Athletes
Authors: Fatt Scott J. , George Emma , Hay Phillipa , Jeacocke Nikki , Gotkiewicz Emily , Mitchison Deborah
Published: July 2024

Background/Objectives: Several systematic reviews and meta-analyses have been conducted to date indicating a high prevalence of disordered eating in elite athletes and associated risk factors. However, the substantial time burden associated with locating and comparing these reviews, which are varied in methodology and sampling focus, may be a barrier for informing policy and best practice as well as directing future research. This umbrella review aimed to provide a summary of evidence across published reviews regarding the prevalence and risk factors for disordered eating (including body image concerns and eating disorders) in elite athletes. Methods: Five databases (CINAHL, PsycINFO, MEDLINE, Scopus, and SPORTDiscus) were systematically searched for peer-reviewed systematic reviews and meta-analyses that met the following inclusion criteria: (1) investigated prevalence and risk factors for disordered eating, (2) included a sample of elite athletes, and (3) available in English. The included studies underwent data extraction and risk of bias assessment using the AMSTAR 2. Results: The initial search identified 1828 articles that were screened for title and abstract and then full text, leaving 24 systematic reviews (including 10 meta-analyses). Disordered eating was prevalent across elite athletes, including males and females and across sport type. Elite athletes were at elevated risk for disordered eating and eating disorders but at lower risk for body image concerns versus non-athlete controls. Several risk factors were identified, including female gender, competing in lean sports, and experiencing career changes. Few reviews or meta-analyses examined perceived pressures within the sporting environment, and most had substantial risk of bias concerns. Conclusions: Elite athletes are at risk for the spectrum of disordered eating, and all should be considered for primary prevention and screening. Further research is needed regarding sport-specific versus general pressures and mediators of risk to better inform interventions.