bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanoparticle-Containing Wound Dressing: Antimicrobial and Healing Effects
Authors: Yudaev Pavel , Mezhuev Yaroslav , Chistyakov Evgeniy
Published: May 2022

The dressings containing nanoparticles of metals and metal oxides are promising types of materials for wound repair. In such dressings, biocompatible and nontoxic hydrophilic polymers are used as a matrix. In the present review, we take a look at the anti-microbial effect of the nanoparticle-modified wound dressings against various microorganisms and evaluate their healing action. A detailed analysis of 31 sources published in 2021 and 2022 was performed. Furthermore, a trend for development of modern antibacterial wound-healing nanomaterials was shown as exemplified in publications starting from 2018. The review may be helpful for researchers working in the areas of biotechnology, medicine, epidemiology, material science and other fields aimed at the improvement of the quality of life.
Development of Bigels Based on Date Palm-Derived Cellulose Nanocrystal-Reinforced Guar Gum Hydrogel and Sesame Oil/Candelilla Wax Oleogel as Delivery Vehicles for Moxifloxacin
Authors: Shaikh Hamid M. , Anis Arfat , Poulose Anesh Manjaly , Madhar Niyaz Ahamad , Al-Zahrani Saeed M.
Published: May 2022

Bigels are biphasic semisolid systems that have been explored as delivery vehicles in the food and pharmaceutical industries. These formulations are highly stable and have a longer shelf-life than emulsions. Similarly, cellulose-based hydrogels are considered to be ideal for these formulations due to their biocompatibility and flexibility to mold into various shapes. Accordingly, in the present study, the properties of an optimized guar gum hydrogel and sesame oil/candelilla wax oleogel-based bigel were tailored using date palm-derived cellulose nanocrystals (dp-CNC). These bigels were then explored as carriers for the bioactive molecule moxifloxacin hydrochloride (MH). The preparation of the bigels was achieved by mixing guar gum hydrogel and sesame oil/candelilla wax oleogel. Polarizing microscopy suggested the formation of the hydrogel-in-oleogel type of bigels. An alteration in the dp-CNC content affected the size distribution of the hydrogel phase within the oleogel phase. The colorimetry studies revealed the yellowish-white color of the samples. There were no significant changes in the FTIR functional group positions even after the addition of dp-CNC. In general, the incorporation of dp-CNC resulted in a decrease in the impedance values, except BG3 that had 15 mg dp-CNC in 20 g bigel. The BG3 formulation showed the highest firmness and fluidity. The release of MH from the bigels was quasi-Fickian diffusion mediated. BG3 showed the highest release of the drug. In summary, dp-CNC can be used as a novel reinforcing agent for bigels.
The Arkalochori Mw = 5.9 Earthquake of 27 September 2021 Inside the Heraklion Basin: A Shallow, Blind Rupture Event Highlighting the Orthogonal Extension of Central Crete
Authors: Ganas Athanassios , Hamiel Yariv , Serpetsidaki Anna , Briole Pierre , Valkaniotis Sotiris , Fassoulas Charalampos , Piatibratova Oksana , Kranis Haralambos , Tsironi Varvara , Karamitros Ioannis , Elias Panagiotis , Vassilakis Emmanuel
Published: May 2022

A strong, shallow earthquake occurred near Heraklion (Crete, Greece) on 27 September 2021. The earthquake produced significant ground deformation in the vicinity of Arkalochori village but without any evidence for surface ruptures of primary origin. We used geodetic (InSAR and GNSS) data to map motions of the Earth’s surface that occurred during and shortly after the earthquake. A 14 cm subsidence of the GNSS station ARKL and a maximum of 19 cm distance from the SAR satellite were recorded. The measured surface displacements were used to constrain the rupture geometry and slip distribution at depth. Our best-fitting inversion model suggests that the rupture occurred on a 13 km-long planar normal fault striking N195° E dipping 55° to the northwest, with major slip occurring to the east and updip of the hypocentre. The fault tip is located 1.2 km beneath the surface. The maximum coseismic slip occurred in the uppermost crust, in the depth interval of 4–6 km. A decrease in the fault offsets toward the Earth’s surface is likely caused by an increased frictional resistance of the shallow layers to rapid coseismic slip. Satellite observations made in the first month after the earthquake detected no post-seismic deformation (i.e., below one fringe or 2.8 cm). The seismic fault may be identified with the Avli (Lagouta) segment of the NNE-SSW striking, west-dipping, 23 km-long neotectonic Kastelli Fault Zone (KFZ). Part of the rupture occurred along the Kastelli segment, indicating a fault segment linkage and a history of overlapping ruptures along KFZ. Based on geological data and footwall topography we estimate an average slip rate between 0.17–0.26 mm/yr for the KFZ. The Arkalochori earthquake is a paradigm example for the on-going extension of Heraklion basin (central Crete) in the WNW-ESE direction, which is almost orthogonal to the E-W Messara graben and other active faults along the south coast of Crete.
Future Orientation and Symptoms of Anxiety and Depression in Italian University Students during the COVID-19 Pandemic: The Role of Resilience and the Perceived Threat of COVID-19
Authors: Servidio Rocco , Scaffidi Abbate Costanza , Costabile Angela , Boca Stefano
Published: May 2022

Several studies have already examined the psychological impact of COVID-19 on psychological well-being in samples of the general population. However, given the importance of future orientation for university students’ mental well-being, it is important to explore whether and how COVID-19 affected this vulnerable population. Therefore, the current study aims to investigate the protective role of future orientation and the mediating effect of resilience on negative emotional symptoms (anxiety and depression). An online questionnaire was administered to a sample of 244 non-infected Italian university students. The mediation analysis results indicated that resilience fully mediated the relationship between anxiety and depression. Finally, the perceived threat of death from COVID-19 moderated the association between resilience and anxiety. This study shows that university students with higher levels of future orientation exhibit higher resilience levels and, consequently, fewer symptoms of negative emotions.
Post-Pandemic Stress Disorder as an Effect of the Epidemiological Situation Related to the COVID-19 Pandemic
Authors: Łaskawiec Daria , Grajek Mateusz , Szlacheta Patryk , Korzonek-Szlacheta Ilona
Published: May 2022

According to many experts in the fields of psychology and psychiatry, the destabilization resulting from the coronavirus pandemic may not be as noticeable now as it will be after the pandemic period is over. Undoubtedly, the fact that the surrounding reality is standardized and normalized by many at present contributes to this. In the opinion of many researchers, the scale and degree of trauma experienced by society will only be noticed by many once the pandemic is over. Many also suggest that we will experience post-pandemic stress disorder. This literature review aims to bring together in one place the information that speaks to the nature of the problem, which is post-pandemic stress disorder. The main sections of the paper deal with exposure to the disorder in the general population and a review of the current literature on the subject. The second section deals with a group of medical personnel who are on the direct frontline in the fight against the COVID-19 pandemic; it is assumed here that they are those who are at much higher risk of developing post-pandemic stress disorder.
Review of Active Extracorporeal Medical Devices to Counteract Freezing of Gait in Patients with Parkinson Disease
Authors: Huerta Mónica , Barzallo Boris , Punin Catalina , Garcia-Cedeño Andrea , Clotet Roger
Published: May 2022

Parkinson Disease (PD) primarily affects older adults. It is the second-most common neurodegenerative disease after Alzheimer’s disease. Currently, more than 10 million people suffer from PD, and this number is expected to grow, considering the increasing global longevity. Freezing of Gait (FoG) is a symptom present in approximately 80% of advanced-stage PD’s patients. FoG episodes alter the continuity of gait, and may be the cause of falls that can lead to injuries and even death. The recent advances in the development of hardware and software systems for the monitoring, stimulus, or rehabilitation of patients with FoG has been of great interest to researchers because detection and minimization of the duration of FoG events is an important factor in improving the quality of life. This article presents a review of the research on non-invasive medical devices for FoG, focusing on the acquisition, processing, and stimulation approaches used.
A Research Based on Online Medical Platform: The Influence of Strong and Weak Ties Information on Patients’ Consultation Behavior
Authors: Zhang Yuting , Qiu Chutong , Zhang Jiantong
Published: May 2022

As an indispensable part of contemporary medical services, Internet-based medical platforms can provide patients with a full range of multi-disciplinary and multi-modal treatment services. Along with the emergence of many healthcare influencers and the increasing connection between online and offline consultations, the operation of individual physicians and their teams on Internet-based medical platforms has started to attract a lot of attention. The purpose of this paper is to, based on an Internet platform, study how the information on physicians’ homepages influences patients’ consultation behavior, so as to provide suggestions for the construction of physicians’ personal websites. We distinguish variables into strong- and weak-ties types, dependent on whether deep social interactions between physicians and patients have happened. If there exist further social interactions, we define the variable as the “strong ties” type, otherwise, “weak ties”. The patients’ consultation behavior will be expressed as the volume of online consultation, i.e., the number of patients. We obtained the strong and weak ties information of each physician based on EWM (entropy weight method), so as to establish a regression model with explained variable, i.e., the number of patients, and three explanatory variables, i.e., the strong and weak ties information, and their interaction term. The estimation results verified our hypotheses and proved to be robust. It showed that both strong and weak ties information can positively influence patients’ consultation behavior, and the influence of weak ties information is greater. Regarding the positive influence of strong and weak ties, we found a trade off effect between them. Based on the results, we finalize with some suggestions on how to improve a physician’s online medical consultation volume.
Symptoms of Burnout Syndrome among Physicians during the Outbreak of COVID-19 Pandemic—A Systematic Literature Review
Authors: Claponea Roxana Mihaela , Pop Lavinia Maria , Iorga Magdalena , Iurcov Raluca
Published: May 2022

Background: Studies in the recent decades show that the medical profession has a high risk to develop burnout due to constant exposure to mental and physical suffering or death. The pandemic period induced additional stress for healthcare professionals due to the likelihood of a high rate of infection, long working shifts, using protective equipment, staying away from family, implementing new medical procedures. The present study is focusing on assessing the prevalence of burnout among physicians working in the healthcare system during the COVID-19 pandemic, and discovering the main factors associated with burnout syndrome among the population of physicians. Material and methods: A systematic review was conducted by searching PubMed, Wiley, and Google Scholar in November 2021. A total of 35 studies were eligible for the evaluation. Results: The samples ranged from 39 to 3071 physicians, and the overall burnout ranged from 14.7% to 90.4%. Sociodemographic characteristics associated with a high prevalence of burnout were the female gender, less experienced, not having children, and single marital status, associated with high levels of anxiety, depression, and stress in the female gender. The highest level of burnout among all the studies was 90.4% on a sample of physicians from the Republic of Korea, 80.2% among psychiatrists in Saudi Arabia, followed by a study in Ireland with a 77% level of burnout among senior and specialist physicians, and 74.7% prevalence of burnout for emergency physicians in USA. Conclusions: During the pandemic, the factors that contribute to burnout are the lack of personal protective equipment and the violence of issues related to organizational health; the high prevalence of burnout symptoms is associated with anxiety, depression, and stress.
Forecasting Hospital Readmissions with Machine Learning
Authors: Michailidis Panagiotis , Dimitriadou Athanasia , Papadimitriou Theophilos , Gogas Periklis
Published: May 2022

Hospital readmissions are regarded as a compounding economic factor for healthcare systems. In fact, the readmission rate is used in many countries as an indicator of the quality of services provided by a health institution. The ability to forecast patients’ readmissions allows for timely intervention and better post-discharge strategies, preventing future life-threatening events, and reducing medical costs to either the patient or the healthcare system. In this paper, four machine learning models are used to forecast readmissions: support vector machines with a linear kernel, support vector machines with an RBF kernel, balanced random forests, and weighted random forests. The dataset consists of 11,172 actual records of hospitalizations obtained from the General Hospital of Komotini “Sismanogleio” with a total of 24 independent variables. Each record is composed of administrative, medical-clinical, and operational variables. The experimental results indicate that the balanced random forest model outperforms the competition, reaching a sensitivity of 0.70 and an AUC value of 0.78.
Deep Feature Extraction for Cymbidium Species Classification Using Global–Local CNN
Authors: Fu Qiaojuan , Zhang Xiaoying , Zhao Fukang , Ruan Ruoxin , Qian Lihua , Li Chunnan
Published: May 2022

Cymbidium is the most famous and widely distributed type of plant in the Orchidaceae family. It has extremely high ornamental and economic value. With the continuous development of the Cymbidium industry in recent years, it has become increasingly difficult to classify, identify, develop, and utilize orchids. In this study, a classification model GL-CNN based on a convolutional neural network was proposed to solve the problem of Cymbidium classification. First, the image set was expanded by four methods (mirror rotation, salt-and-pepper noise, image sharpening, and random angle flip), and then a cascade fusion strategy was used to fit the multiscale features obtained from the two branches. Comparing the performance of GL-CNN with other four classic models (AlexNet, ResNet50, GoogleNet, and VGG16), the results showed that GL-CNN achieves the highest classification prediction accuracy with a value of 94.13%. This model can effectively detect different species of Cymbidium and provide a reference for the identification of Cymbidium germplasm resources.
Origins of Sulfate in Groundwater and Surface Water of the Rio Grande Floodplain, Texas, USA and Chihuahua, Mexico
Authors: Eastoe Christopher , Hibbs Barry , Merino Mercedes , Dadakis Jason
Published: May 2022

Sulfate isotopes (δ34S, δ18OSO4) interpreted in conjunction with sulfate concentrations show that sulfate of both agricultural and geologic sources is present in groundwater and surface water in the Rio Grande flood plain within the Hueco Bolsón. From previous studies, water isotopes (δ2H, δ18O) in the study area indicate groundwater age relative to dam construction upstream. Surface water entering the Hueco Bolsón contains a mixture of soil-amendment sulfate and sulfate from deep-basin groundwater seeps at the terminus of Mesilla Valley. In the shallow Rio Grande alluvial aquifer within the Hueco Bolsón, ranges of δ34S in pre-dam (+2 to +9‰) and post-dam (0 to +6‰) groundwater overlap; the range for post-dam water coincides with common high-sulfate soil amendments used in the area. Most post-dam groundwater, including discharge into agricultural drains, has higher sulfate than pre-dam groundwater. In surface water downstream of Fabens, high-δ34S (>+10‰) sulfate, resembling Middle Permian gypsum, mixes with sulfate from upstream sources and agriculture. The high- δ34S sulfate probably represents discharge from the regional Hueco Bolsón aquifer. In surface water downstream of Fort Hancock, soil-amendment sulfate predominates, probably representing discharge from the Rio Grande alluvial aquifer near the basin terminus. The δ18OSO4 dataset is consistent with sulfate origins determined from the larger δ34S dataset.
Quality of Work Life and Work Process of Assistance Nurses
Authors: Parra-Giordano Denisse , Quijada Sánchez Denisse , Grau Mascayano Patricia , Pinto-Galleguillos Daniela
Published: May 2022

Background: The concept of Quality of Work Life (QWL) has been built multidimensionally through social reproduction; it is impacted by the perceptions of each individual and by the relationship between workers and the work environment. Objective: to analyze the Work Process and QWL of assisting nurses in public health. Methods: Research in a critical paradigm, descriptive, exploratory with a qualitative approach. The population corresponds to nurses who work in care work. Semi-structured guiding questions were applied and were analyzed with content analysis. Results: seven participants declared female; all Chilean; seven are young adults; six singles; only one has children, and one has a person dependent on her care; six are heads of household, and five receive help with housework. All have a nursing degree, five have a diploma, but none have a postgraduate. The work process has three subcategories: work object, instrument, organization, and work conditions; the QWL category has six subcategories: definition and perception of QWL, QWL potentiating factors, QWL exhausting factors, QWL improvement strategies, the emotional burden associated with QWL, and health problems. Conclusions: In this way, the lifestyle built by the assistance in the health area has repercussions on the quality of life and health in general.
Impact of the Pandemic on the Teaching and Research Staff at a Technological University in Spain: Deepening the Gender Gap
Authors: Peña Marta , Olmedo-Torre Noelia , Alcaraz Olga , Chavez-Dominguez Juan A. , López José , Mujica Luis Eduardo
Published: May 2022

The alteration of the educational model caused by the COVID-19 pandemic has not affected all university faculty equally. This work explores the academic, digital and gender inequalities caused by the pandemic on the teaching and research staff of a technological university for STEM (Science, Technology, Engineering and Mathematics) disciplines in Spain, the Universitat Politècnica de Catalunya—BarcelonaTech (UPC). The study considers an anonymous survey with a non-probabilistic voluntary sample (n = 355). The results of the survey reveal that, over these months, the teaching and research staff of the university, regardless of gender, has significantly increased its academic activity due especially to the number of hours devoted to virtual teaching compared to its teaching dedication in a situation of normalcy. This study shows that the lockdown has strongly affected women who are more vulnerable to crisis. In particular, the negative impact on research has been higher in female faculty staff from the UPC, who already face disparities regarding promotion and, during lockdown, stated more difficulties with household work reconciliation. From the results of this study, it is possible to conclude that the COVID-19 pandemic has deepened the gender gap in the academic field.
Level of Serum Fetuin-A Correlates with Heart Rate in Obstructive Sleep Apnea Patients without Metabolic and Cardiovascular Comorbidities
Authors: Reichert Elżbieta , Mosiewicz Jerzy , Myśliński Wojciech , Jaroszyński Andrzej , Stanek Agata , Brożyna-Tkaczyk Klaudia , Madejska-Mosiewicz Barbara
Published: May 2022

Obstructive sleep apnea (OSA) is the most common type of sleep-induced breathing disorder in the adult population and significantly affects the condition of the cardiovascular system. Fetuin-A (Fet-A) is a hepato- and adipokine, which prevents vessel calcification, and its level correlates with atherogenesis and metabolic disorders. The associations of cardiovascular diseases (CVD) both with OSA, which increases CVD risk, and Fet-A, which prevents CVD, justify the question of their mutual interactions in patients with OSA. Therefore, we sought to analyze Fet-A as an early biomarker of CVD risk in OSA patients without metabolic and cardiovascular comorbidities except for properly controlled arterial hypertension. We have found that in these patients, OSA does not appear to directly affect Fet-A levels. However, high Fet-A levels were more common in the group of patients with OSA, and the hypopnea index was significantly higher among subjects with the highest Fet-A levels. The level of Fet-A in OSA patients positively correlates with pulse rate, and it does not correlate with pulse pressure in this group unlike in the control group, where such a relationship exists. To our best knowledge, this is the first study to analyze this relationship in OSA patients without any significant cardiovascular comorbidities.
Alzheimer Caregiving Problems According to ADLs: Evidence from Facebook Support Groups
Authors: Bachmann Pavel , Hruska Jan
Published: May 2022

Background and study goal: Social media are a societal phenomenon today, including the oldest generation, yet they are seldom used in current health research to identify the needs of persons with Alzheimer’s disease (PADs) and their carers. There is an even bigger research gap in the analysis of caregivers’ communication in online support groups and its classification according to activities of daily living (ADLs). For this, the goal of this study is to identify real-life practices of informal caregivers who care for PADs based on the analysis of their communication in Facebook groups. Methods: A sample of 1603 contributions was obtained from support groups by keyword search, manual coding, and verification; thus, the contributions in the sample are relevant for the individual basic ADLs of PADs. Next, five main conversation topics were identified for each ADL. This was done using the topic extractor based on simple parallel threaded implementation of LDA with a sparse sampling scheme and data structure. Results: The qualitative dimension of research identified discussion topics as well as specific caregiver behavior patterns for each ADL. The quantitative dimension determining the level of engagement of group members in individual ADLs was also measured. The highest engagement was found in activities of feeding and drinking, followed by bathing. In contrast, the activities of dressing, continence, and toileting attract the lowest interest. Moreover, the causal links between the topics discussed within the areas of ADLs were identified. Conclusions: The acquired knowledge can help further research focus on the most problematic areas relevant for people with AD in order to increase their quality of life and at the same time reduce the caregiver burden. The study expands the information of the demands posed by the individual caregiver activities, specifically in the context of activity-based costing or time-based activity costing.
Connecting Healthcare with Income Maximisation Services: A Systematic Review on the Health, Wellbeing and Financial Impacts for Families with Young Children
Authors: Burley Jade , Samir Nora , Price Anna , Parker Anneka , Zhu Anna , Eapen Valsamma , Contreras-Suarez Diana , Schreurs Natalie , Lawson Kenny , Lingam Raghu , Grace Rebekah , Raman Shanti , Kemp Lynn , Bishop Rebecca , Goldfeld Sharon , Woolfenden Susan
Published: May 2022

Financial counselling and income-maximisation services have the potential to reduce financial hardship and its associated burdens on health and wellbeing in High Income Countries. However, referrals to financial counselling services are not systematically integrated into existing health service platforms, thus limiting our ability to identify and link families who might be experiencing financial hardship. Review evidence on this is scarce. The purpose of this study is to review “healthcare-income maximisation” models of care in high-income countries for families of children aged between 0 and 5 years experiencing financial difficulties, and their impacts on family finances and the health and wellbeing of parent(s)/caregiver(s) or children. A systematic review of the MEDLINE, EMBase, PsycInfo, CINAHL, ProQuest, Family & Society Studies Worldwide, Cochrane Library, and Informit Online databases was conducted according to the Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) statement. A total of six studies (five unique samples) met inclusion criteria, which reported a total of 11,603 families exposed to a healthcare-income maximisation model. An average annual gain per person of £1661 and £1919 was reported in two studies reporting one Scottish before–after study, whereby health visitors/midwives referred 4805 clients to money advice services. In another UK before–after study, financial counsellors were attached to urban primary healthcare centres and reported an average annual gain per person of £1058. The randomized controlled trial included in the review reported no evidence of impacts on financial or non-financial outcomes, or maternal health outcomes, but did observe small to moderate effects on child health and well-being. Small to moderate benefits were seen in areas relating to child health, preschool education, parenting, child abuse, and early behavioral adjustment. There was a high level of bias in most studies, and insufficient evidence to evaluate the effectiveness of healthcare-income maximisation models of care. Rigorous (RCT-level) studies with clear evaluations are needed to assess efficacy and effectiveness.
Health Impact Assessment (HIA) of a Daily Physical Activity Unit in Schools: Focus on Children and Adolescents in Austria Up to the 8th Grade
Authors: Movia Madlene , Macher Sandra , Antony Gabriele , Zeuschner Verena , Wamprechtsamer Gabriela , delle Grazie Judith , Simi Helmut , Fuchs-Neuhold Bianca
Published: May 2022

Physical activity has a variety of health benefits for young people. The World Health Organization (WHO) recommends that children and adolescents aged 5–17 years should be physically active for at least 60 min a day. This Health Impact Assessment (HIA) examined the potential impact of a daily physical activity unit in Austrian schools, with a focus on children and adolescents up to eighth grade. The HIA methodology systematically followed several stages: screening and scoping, appraisal, and formulation of recommendations. A literature review, an online survey (n = 712), focus group discussions (n = 4), and appraisal workshops (n = 2) have been carried out. The analyzed results indicate a primarily positive impact on the individual health of children and adolescents, on social and community-related networks, on living and working conditions, and on the system level. Recommendations for the implementation include ensuring equal opportunities and support for disadvantaged children and schools. Furthermore, the comprehensible selection of pilot regions and the nationwide resource planning should be considered. Additional important factors include the education and further training of pedagogical staff and coaches, and the availability of sufficient infrastructure. In the long term, the fixed integration of five additional teaching units of physical activity per week, and an increased focus on the elementary/nursery school sector were identified as significant.
Cystidicola farionis, a Swim Bladder Parasite of European Smelt: Characterization of the Nematode Trehalose Strategy
Authors: Dmitryjuk Małgorzata , Szczotko Magdalena , Kubiak Katarzyna , Dziekońska-Rynko Janina , Cichocka Joanna , Hliwa Piotr , Mierzejewska Katarzyna
Published: May 2022

The molecular identification of Cystidicola farionis (a swim bladder nematode of European smelt from the Vistula Lagoon in Poland) was performed. Their prevalence level was determined, and changes in the trehalose synthesis pathway in larvae and adult nematodes were demonstrated. The trehalose level was almost four times higher in adult nematodes than in larvae. In contrast, the activity of both enzymes (trehalose 6-phosphate synthase, TPS and trehalose 6-phosphate phosphatase, TPP) involved in the synthesis of trehalose was higher in larvae than in adults under optimal conditions. The optimum pH for TPS isolated from larvae and adults was pH 7.0. The optimum pH for TPP from larvae and adults was pH 7.0 and pH 8.0, respectively. The optimal temperature was 20 °C, and Mg2+ ions were an activator for trehalose-synthetizing enzymes from both sources. Enzymes isolated from adult nematodes were less susceptible to divalent ion chelator and inorganic phosphate than larval enzymes. The dynamic transformation of trehalose in the nematode developing inside the swim bladder of the smelt appears to be an important metabolic pathway in the nematode survival strategy. These studies are aimed at a better understanding of the issue of the metabolic adaptation of parasites, which, in the future, may indirectly contribute to the elimination of the parasite from aquacultures, which will impact public health.
“You Have to Go Gently”: Mentors’ Perspectives of a Peer Mentoring Empowerment Program to Reduce Marginalization in Refugee and Migrant Women
Authors: Gower Shelley , Jeemi Zakia , Dantas Jaya A. R.
Published: May 2022

The Empowerment and Peer Mentoring of Migrant and Refugee Women pilot program (EMPOWER) provides a mechanism for migrant women who have established lives in Australia to mentor newly arrived women to build the ability, confidence, and knowledge to overcome barriers to the social determinants of health such as employment. Female migrant mentors (n = 21) met with their mentees (n = 32) on a regular basis over a period of 3 to 12 months between September 2019 and November 2021. The individual mentoring was augmented by group workshops facilitated by content experts and the research team. The unique perspectives of the mentors were explored through individual interviews (n = 15) and analysis of journal entries (n = 58) submitted regularly by mentors throughout the program. Thematic analysis revealed that mentors were intrinsically motivated to build strong and trusting connections with their mentees, which were pivotal to reducing inequalities for mentees and their families. Mentors had high expectations of themselves and demonstrated commitment and flexibility to accommodate mentees’ needs. However, they sometimes struggled when supporting mentees who were overwhelmed by the systemic and other stressors associated with resettlement and pre-migration trauma. Regular networking and moral support for mentors would enhance future programs.
Special Issue Editorial: “Infections, Inflammation and Neurodegeneration in Alzheimer Disease” Infections, Neuronal Senescence, and Dementia
Authors: Licastro Federico
Published: May 2022

Alzheimer’s disease (AD) is a complex chronic disease of the brain characterized by several neurodegenerative mechanisms and is responsible for most dementia cases in the elderly. Declining immunity during ageing is often associated with peripheral chronic inflammation, and chronic neuroinflammation is a constant component of AD brain pathology. In the Special Issue published in 2021 eight papers were collected regarding different aspects of neurodegeneration associated with AD. Five papers presented and discussed infectious agents involved in brain AD pathology and three discussed data regarding receptors regulation and possible treatment of the disease. Below I will discuss and further elaborate on topics related to infections, inflammation, and neurodegenerative pathways in AD and brain senescence. The topic presented here may contribute to early intervention protocols for preventing or slowing the progression of cognitive deterioration in the elderly.
Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism
Authors: Takasawa Shin , Shobatake Ryogo , Takeda Yoshinori , Uchiyama Tomoko , Yamauchi Akiyo , Makino Mai , Sakuramoto-Tsuchida Sumiyo , Asai Keito , Ota Hiroyo , Itaya-Hironaka Asako
Published: May 2022

Sleep apnea syndrome (SAS), characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia (IH)), is a risk factor for hypertension and insulin resistance. We report a correlation between IH and insulin resistance/diabetes. However, the reason why hypertension is induced by IH is elusive. Here, we investigated the effect of IH on the expression of catecholamine-metabolizing enzymes using an in vitro IH system. Human and mouse neuroblastoma cells (NB-1 and Neuro-2a) were exposed to IH or normoxia for 24 h. Real-time RT-PCR revealed that IH significantly increased the mRNA levels of dopamine β-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in both NB-1 and Neuro-2a. Western blot showed that the expression of DBH and PNMT in the NB-1 cells was significantly increased by IH. Reporter assays revealed that promoter activities of DBH and PNMT were not increased by IH. The miR-375 level of IH-treated cells was significantly decreased relative to that of normoxia-treated cells. The IH-induced up-regulation of DBH and PNMT was abolished by the introduction of the miR-375 mimic, but not by the control RNA. These results indicate that IH stress increases levels of DBH and PNMT via the inhibition of miR-375-mediated mRNA degradation, potentially playing a role in the emergence of hypertension in SAS patients.
Mass Spectrometry-Based Disulfide Mapping of Lysyl Oxidase-like 2
Authors: Meier Alex A. , Go Eden P. , Moon Hee-Jung , Desaire Heather , Mure Minae
Published: May 2022

Lysyl oxidase-like 2 (LOXL2) catalyzes the oxidative deamination of peptidyl lysines and hydroxylysines to promote extracellular matrix remodeling. Aberrant activity of LOXL2 has been associated with organ fibrosis and tumor metastasis. The lysine tyrosylquinone (LTQ) cofactor is derived from Lys653 and Tyr689 in the amine oxidase domain via post-translational modification. Based on the similarity in hydrodynamic radius and radius of gyration, we recently proposed that the overall structures of the mature LOXL2 (containing LTQ) and the precursor LOXL2 (no LTQ) are very similar. In this study, we conducted a mass spectrometry-based disulfide mapping analysis of recombinant LOXL2 in three forms: a full-length LOXL2 (fl-LOXL2) containing a nearly stoichiometric amount of LTQ, Δ1-2SRCR-LOXL2 (SRCR1 and SRCR2 are truncated) in the precursor form, and Δ1-3SRCR-LOXL2 (SRCR1, SRCR2, SRCR3 are truncated) in a mixture of the precursor and the mature forms. We detected a set of five disulfide bonds that is conserved in both the precursor and the mature recombinant LOXL2s. In addition, we detected a set of four alternative disulfide bonds in low abundance that is not associated with the mature LOXL2. These results suggest that the major set of five disulfide bonds is retained post-LTQ formation.
Amentoflavone Promotes Cellular Uptake and Degradation of Amyloid-Beta in Neuronal Cells
Authors: Han Byung Hee , Cofell Brooke , Everhart Emily , Humpal Courtney , Kang Sam-Sik , Lee Sang Kook , Kim-Han Jeong Sook
Published: May 2022

Deposition of fibrillar forms of amyloid β-protein (Aβ) is commonly found in patients with Alzheimer’s disease (AD) associated with cognitive decline. Impaired clearance of Aβ species is thought to be a major cause of late-onset sporadic AD. Aβ secreted into the extracellular milieu can be cleared from the brain through multiple pathways, including cellular uptake in neuronal and non-neuronal cells. Recent studies have showed that the naturally-occurring polyphenol amentoflavone (AMF) exerts anti-amyloidogenic effects. However, its effects on metabolism and cellular clearance of Aβ remain to be tested. In the present study, we demonstrated that AMF significantly increased the cellular uptake of both Aβ1-40 and Aβ1-42, but not inverted Aβ42-1 in mouse neuronal N2a cells. Though AMF promoted internalization of cytotoxic Aβ1-42, it significantly reduced cell death in our assay condition. Our data further revealed that the internalized Aβ is translocated to lysosomes and undergoes enzymatic degradation. The saturable kinetic of Aβ uptake and our pharmacologic experiments showed the involvement of receptor-mediated endocytosis, in part, through the class A scavenger receptors as a possible mechanism of action of AMF. Taken together, our findings indicate that AMF can lower the levels of extracellular Aβ by increasing their cellular uptake and clearance, suggesting the therapeutic potential of AMF for the treatment of AD.
The Common Cellular Events in the Neurodegenerative Diseases and the Associated Role of Endoplasmic Reticulum Stress
Authors: Kim Soojeong , Kim Doo Kyung , Jeong Seho , Lee Jaemin
Published: May 2022

Neurodegenerative diseases are inseparably linked with aging and increase as life expectancy extends. There are common dysfunctions in various cellular events shared among neurogenerative diseases, such as calcium dyshomeostasis, neuroinflammation, and age-associated decline in the autophagy-lysosome system. However, most of all, the prominent pathological feature of neurodegenerative diseases is the toxic buildup of misfolded protein aggregates and inclusion bodies accompanied by an impairment in proteostasis. Recent studies have suggested a close association between endoplasmic reticulum (ER) stress and neurodegenerative pathology in cellular and animal models as well as in human patients. The contribution of mutant or misfolded protein-triggered ER stress and its associated signaling events, such as unfolded protein response (UPR), to the pathophysiology of various neurodegenerative disorders, including Alzheimer’s, Parkinson’s, and Huntington’s disease, amyotrophic lateral sclerosis, and prion disease, is described here. Impaired UPR action is commonly attributed to exacerbated ER stress, pathogenic protein aggregate accumulation, and deteriorating neurodegenerative pathologies. Thus, activating certain UPR components has been shown to alleviate ER stress and its associated neurodegeneration. However, uncontrolled activation of some UPR factors has also been demonstrated to worsen neurodegenerative phenotypes, suggesting that detailed molecular mechanisms around ER stress and its related neurodegenerations should be understood to develop effective therapeutics against aging-associated neurological syndromes. We also discuss current therapeutic endeavors, such as the development of small molecules that selectively target individual UPR components and address ER stress in general.
Pseudomonas putida Biofilm Depends on the vWFa-Domain of LapA in Peptides-Containing Growth Medium
Authors: Puhm Marge , Hendrikson Johanna , Kivisaar Maia , Teras Riho
Published: May 2022

The biofilm of Pseudomonas putida is complexly regulated by several intercellular and extracellular factors. The cell surface adhesin LapA of this bacterium is a central factor for the biofilm and, consequently, the regulation of lapA expression, for example, by Fis. It has been recently shown that peptides in growth media enhance the formation of P. putida biofilm, but not as a source of carbon and nitrogen. Moreover, the peptide-dependent biofilm appeared especially clearly in the fis-overexpression strain, which also has increased LapA. Therefore, we investigate here whether there is a relationship between LapA and peptide-dependent biofilm. The P. putida strains with inducible lapA expression and LapA without the vWFa domain, which is described as a domain similar to von Willebrand factor domain A, were constructed. Thereafter, the biofilm of these strains was assessed in growth media containing extracellular peptides in the shape of tryptone and without it. We show that the vWFa domain in LapA is necessary for biofilm enhancement by the extracellular peptides in the growth medium. The importance of vWFa in LapA was particularly evident for the fis-overexpression strain F15. The absence of the vWFa domain diminished the positive effect of Fis on the F15 biofilm.
Ameliorative Effects of Lactobacillus plantarum HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition
Authors: Kim Jong-Yeon , Park Eun-Jung , Lee Hae-Jeung
Published: May 2022

Lactobacillus plantarum HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of L. plantarum HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of L. plantarum HAC01 lysate and its associated mechanism of action. To induce lipid accumulation, 3T3-L1 cells were incubated in differentiation media with or without L. plantarum HAC01 lysate. Our results show that L. plantarum HAC01 lysate treatment not only reduced lipid accumulation during the differentiation of 3T3-L1 cells, but also decreased the expression of adipogenic and lipogenic genes involved in lipid metabolism in a dose-dependent manner. Additionally, L. plantarum HAC01 lysate inhibited CCAAT/enhancer-binding protein (C/EBP) beta within 4 h of differentiation induction and inhibited peroxisome proliferator-activated receptor gamma, C/EBP alpha, and sterol regulatory element-binding proteins within 2 d. Moreover, treatment with L. plantarum HAC01 lysate increased the phosphorylation of adenosine monophosphate-activated protein kinase, an important regulator of energy metabolism, and decreased the phosphorylation of mitogen-activated protein kinase. These results indicate that L. plantarum HAC01 lysate may have anti-adipogenic effects and support its potential as a useful agent for the treatment of obesity.
The Second Class of Tetrahydrofolate (THF-II) Riboswitches Recognizes the Tetrahydrofolic Acid Ligand via Local Conformation Changes
Authors: Zhang Minmin , Liu Guangfeng , Zhang Yunlong , Chen Ting , Feng Shanshan , Cai Rujie , Lu Changrui
Published: May 2022

Riboswitches are regulatory noncoding RNAs found in bacteria, fungi and plants, that modulate gene expressions through structural changes in response to ligand binding. Understanding how ligands interact with riboswitches in solution can shed light on the molecular mechanisms of this ancient regulators. Previous studies showed that riboswitches undergo global conformation changes in response to ligand binding to relay information. Here, we report conformation switching models of the recently discovered tetrahydrofolic acid-responsive second class of tetrahydrofolate (THF-II) riboswitches in response to ligand binding. Using a combination of selective 2′-hydroxyl acylation, analyzed by primer extension (SHAPE) assay, 3D modeling and small-angle X-ray scattering (SAXS), we found that the ligand specifically recognizes and reshapes the THF-II riboswitch loop regions, but does not affect the stability of the P3 helix. Our results show that the THF-II riboswitch undergoes only local conformation changes in response to ligand binding, rearranging the Loop1-P3-Loop2 region and rotating Loop1 from a ~120° angle to a ~75° angle. This distinct conformation changes suggest a unique regulatory mechanism of the THF-II riboswitch, previously unseen in other riboswitches. Our findings may contribute to the fields of RNA sensors and drug design.
Role of Cannabinoid CB2 Receptor in Alcohol Use Disorders: From Animal to Human Studies
Authors: García-Gutiérrez María Salud , Navarrete Francisco , Gasparyan Ani , Navarro Daniela , Morcuende Álvaro , Femenía Teresa , Manzanares Jorge
Published: May 2022

Cumulative evidence has pointed out cannabinoid CB2 receptors (CB2r) as a potential therapeutic key target for treating alcohol use disorder (AUD). This review provides the most relevant results obtained from rodent and human studies, including an integrative section focused on the involvement of CB2r in the neurobiology of alcohol addiction. A literature search was conducted using the electronic databases Medline and Scopus for articles. The search strategy was as follows: “Receptor, Cannabinoid, CB2” AND “Alcohol-Related Disorders” AND “human/or patients”; “Receptor, Cannabinoid, CB2” AND “Alcohol” OR “Ethanol” AND “rodents/or mice/or rats”. Pharmacological approaches demonstrated that the activation or blockade of CB2r modulated different alcohol-addictive behaviors. Rodent models of alcoholism revealed significant alterations of CB2r in brain areas of the reward system. In addition, mice lacking CB2r (CB2KO) show increased alcohol consumption, motivation, and relapse alterations. It has been stressed that the potential neurobiological mechanisms underlying their behavioral effects involve critical elements of the alcohol reward system. Interestingly, recent postmortem studies showed CNR2 alterations in brain areas of alcoholic patients. Moreover, although the number of studies is limited, the results revealed an association between some genetic alterations of the CNR2 and an increased risk for developing AUD. This review provides evidence that CB2r may play a role in alcohol addiction. Clinical studies are necessary to figure out whether CB2r ligands may prove useful for the treatment of AUD in humans.
Response of Poplar and Associated Fungal Endophytic Communities to a PAH Contamination Gradient
Authors: Gréau Lilian , Blaudez Damien , Heintz Dimitri , Zumsteg Julie , Billet David , Cébron Aurélie
Published: May 2022

Microbial populations associated to poplar are well described in non-contaminated and metal-contaminated environments but more poorly in the context of polycyclic aromatic hydrocarbon (PAH) contamination. This study aimed to understand how a gradient of phenanthrene (PHE) contamination affects poplar growth and the fungal microbiome in both soil and plant endosphere (roots, stems and leaves). Plant growth and fitness parameters indicated that the growth of Populus canadensis was impaired when PHE concentration increased above 400 mg kg−1. Values of alpha-diversity indicators of fungal diversity and richness were not affected by the PHE gradient. The PHE contamination had a stronger impact on the fungal community composition in the soil and root compartments compared to that of the aboveground organs. Most of the indicator species whose relative abundance was correlated with PHE contamination decreased along the gradient indicating a toxic effect of PHE on these fungal OTUs (Operational Taxonomic Units). However, the relative abundance of some OTUs such as Cadophora, Alternaria and Aspergillus, potentially linked to PHE degradation or being plant-beneficial taxa, increased along the gradient. Finally, this study allowed a deeper understanding of the dual response of plant and fungal communities in the case of a soil PAH contamination gradient leading to new perspectives on fungal assisted phytoremediation.