bluuebunny/crossref_metadata_2025 huggingface dataset:
PERAN SEKOLAH MENENGAH KEJURUAN DALAM MENGURANGI TINGKAT PENGANGGURAN
Authors: Akbar Muhammad Zaynul , Ichwanto Muhammad Aris , Fatahillah Muhammad Arjuna , Fadhilatuzzahro Hanif , Muthmainnah Muthmainnah
Published: September 2024

Abstrak : Tingginya tingkat pengangguran terbuka tahun 2022 yang di dominasi oleh siswa lulusan pendidikan menengah kejuruan menjadi perhatian penulis untuk melakukan penelitian tentang upaya yang harus dilakukan Sekolah Menengah Kejuruan (SMK) sehingga memiliki peran dalam mengurangi tingkat pengangguran. Penelitian ini menggunakan metode kualitatif dengan tinjauan literature. Beberapa peran yang dapat dilakukan Sekolah Menengah Kejuruan (SMK) terhadap lulusan untuk mengurangi tingkat pengangguran terbuka yaitu: pemenuhan kebutuhan tenaga kerja, pemanfaatan potensi daerah, keterampilan berwirausaha, dan melanjutkan jenjang pendidikan.
IMPLEMENTASI METODE AHP DAN MOORA UNTUK PENGAMBILAN KEPUTUSAN PENENTUAN KREDIT PADA KSPPS AMANAH INSANI MAKMUR BANTUL
Authors: Nuraska Dina , Dewa Weda Adistianaya , Sophia Evy
Published: September 2024

Abstrak : Koperasi Simpan Pinjam Pola Syariah (SPSLC) Amanah Insani Makmur yang berlokasi di Gedongan, Panjangrejo, Kecamatan Pundong, Kabupaten Bantul, Daerah Istimewa Yogyakarta, mengalami peningkatan kredit macet sebesar 32 percent akibat proses pengambilan keputusan yang kurang optimal, bersifat subjektif, dan tidak mempertimbangkan riwayat transaksi anggota. Untuk mengatasi masalah ini, diperlukan perubahan kebijakan dalam pengambilan keputusan dengan pendekatan berbasis data, yaitu dengan menggabungkan metode AHP (Analytical Hierarchy Process) untuk pembobotan kriteria dan MOORA (Multi-Objective Optimization on the basis of Ratio Analysis) untuk pemeringkatan alternatif. Pengujian dengan 30 sampel data menunjukkan bahwa pendekatan gabungan ini menghasilkan penilaian kredit yang lebih realistis dan objektif.
Dampak Penggunaan E-commerce Terhadap Kalangan Remaja pada Society 5.0
Authors: Saffa Dhamira Faradhila , Handayani Anik Nur
Published: September 2024

In the increasingly advanced era of Society 5.0, the use of e-commerce is becoming more widespread, including among teenagers. However, the impact of e-commerce use on teenagers is still a concern for many. This article discusses the impact of e-commerce use on teenagers in Society 5.0. Along with the increasing use of e-commerce by teenagers, this article discusses the positive and negative impacts of e-commerce use on teenagers and society. In addition, this article also discusses efforts that can be made to minimize the negative impacts of e-commerce use on teenagers. Through this article, it is hoped to gain a more complete understanding of e-commerce use among teenagers in Society 5.0 and to help society make wiser decisions in using this technology.
Dalam era Society 5.0 yang semakin maju, penggunaan e-commerce semakin meluas, termasuk di kalangan remaja. Namun, dampak penggunaan e-commerce terhadap kalangan remaja masih menjadi perhatian banyak orang. Artikel ini membahas tentang dampak penggunaan e-commerce terhadap kalangan remaja pada Society 5.0. Seiring dengan meningkatnya penggunaan e-commerce oleh remaja, artikel ini mengulas dampak positif dan negatif dari penggunaan e-commerce terhadap remaja dan masyarakat secara keseluruhan. Selain itu, artikel ini juga membahas tentang upaya yang dapat dilakukan untuk meminimalisir dampak negatif dari penggunaan e-commerce bagi kalangan remaja. Melalui artikel ini diharapkan dapat memperoleh pengetahuan yang lebih lengkap mengenai penggunaan e-commerce di kalangan remaja dalam era Society 5.0 dan membantu masyarakat untuk mengambil keputusan yang lebih bijak dalam menggunakan teknologi tersebut.
Pentingnya Pengetahuan dan Kemampuan di Era Society 5.0
Authors: Kurniawan Rizky Rizaldi , Wibawa Aji
Published: September 2024

This article aims to find out how to adapt in the area of society 5.0, where the way to adapt discussed in this article is with knowledge and abilities. The qualitative method is the method used in this article. This type of research is used because research discusses the importance of knowledge and abilities in the area of society 5.0, so it requires journals to carry out result and discussions and obtain conclusions. The procedure section in this study has 2 steps, namely: searching for journals related to knowledge and abilities in the area of society 5.0 and reading and analyzing these journals with these steps, result of journal analysis related to titles can be obtained so that they can be used in result and discussion. The data analysis instrumens and techniques in this article are documents. In this study, the results were obtained, namely knowledge and abilitied are important things to have so that they can adapt to the era of society 5.0. Knowledge is understood as wee as ability. This is so as not to be unrivaled by others so that they can adapt.
Artikel ini bertujuan untuk mengetahui cara beradaptasi di era society 5.0 yang dimana cara beradaptasi yang dibahas dalam artikel ini yaitu dengan adanya pengetahuan dan kemampuan. Metode kualitatif menjadi metode yang dipakai dalam artikel ini. Jenis penelitian ini digunakan karena penelitian membahas tentang pentingnya pengetahuan dan kemampuan pada era society 5.0 sehingga memerlukan jurnal-jurnal untuk melakukan hasil dan pembahasan serta memperoleh kesimpulan. Bagian prosedur pada penelitian ini terdapat 2 langkah yaitu: mencari jurnal yang berkaitan dengan pengetahuan dan kemampuan di era society 5.0 dan membaca serta menganalisis jurnal tersebut dengan langkah-langkah tersebut maka dapat diperoleh hasil analisis jurnal yang berkaitan dengan judul sehingga dapat digunakan dalam hasil dan pembahasan. Instrumen dan teknik analisis data dalam artikel ini yaitu dokumen. Pada penelitian ini memperoleh hasil yaitu pengetahuan dan kemampuan menjadi hal yang penting dimiliki sehingga dapat beradaptasi pada era society 5.0. Pengetahuan benar-benar dipahami begitu juga dengan kemampuan. Hal ini dikarenakan agar tidak kalah tersaingi dengan orang lain sehingga bisa beradaptasi.
Perdagangan Elektronik Pada Industri 4.0 dan Society 5.0
Authors: Naufal Farras Muhammad , Ar Rosyid Harits
Published: September 2024

The main topics of this paper are e-commerce, Industry 4.0, as well as Society 5.0, which includes terms, history, workflow, as well as technological aspects. A comparative analysis between Industry 4.0 and Society 5.0 was conducted to investigate their alignment impact towards e-commerce. After thorough examination and comparison, the study concluded that the technology in Society 5.0 is part of the continuation of Industry 4.0 technology. Furthermore, there is technological synergy between electronic commerce as well as the industry 4.0 period and Society 5.0, with electronic commerce technology adapting to the technology of each era. The development and progress of electronic commerce will benefit greatly from Industry 4.0 and Society 5.0.
Topik utama makalah ini adalah perdagangan elektronik, Industri 4.0, juga Masyarakat 5.0, yang termasuk dalam istilah, sejarah, rangkaian kerja, juga aspek teknologinya. Analisis komparatif antara Industri 4.0 dan Masyarakat 5.0 dilakukan untuk menyelidiki dampak keselarasan mereka yang mengarahkan kepada e-commerce. Setelah dilakukan pemeriksaan dan perbandingan secara menyeluruh, kajian tersebut menyimpulkan yaitu, teknologi yang ada pada Society 5.0 bagian dari lanjutan yang berasal dari teknologi Industri 4.0. Selanjutnya, terdapat sinergi teknologi antar perdagangan elektronik juga periode Industri 4.0 dengan Masyarakat 5.0, dengan teknologi perdagangan elektronik yang beradaptasi dengan teknologi setiap era. Perkembangan dan kemajuan perdagangan elektronik sangat diuntungkan oleh Industri 4.0 dan Masyarakat 5.0.
Kirkpatrick's Evaluation Model for The Implementation of Industrial Standard GTAW Welding in Vocational Teacher Training Center
Authors: Farida Khotimatul , Nurhadi Didik
Published: September 2024

The Vocational Teacher Training Center held industrial-based training by applying project-based learning to train Industrial Standard GTAW Welding. Then, this study was carried out to evaluate the effectiveness of training implementation of Industrial Standard GTAW Welding. This study used the Kirkpatrick Evaluation Model in reaction and learning levels. A questionnaire and interview were chosen to collect data. The data were analyzed by using descriptive technique, t-test, and qualitative data analysis with NVivo. The findings indicated that the participants' satisfaction scores in reaction and learning levels were very high. The assessment of the participants at the reaction level was seen from three perspectives: instructors’ reaction, reaction to the training delivery, and reaction to the training environment. Assessment of participants at the learning level was viewed from the impact of learning and knowledge training. The training proved that the program has been successful in developing the trainees’ skills. This also showed that the program has been running effectively. However, the final products from trainees did not meet the industry's standards. Optimizing this training requires additional time and standardization of participants' abilities using portfolios from training candidates.
Balai Diklat Guru SMK menyelenggarakan pelatihan berbasis industri dengan menerapkan pembelajaran berbasis proyek untuk melatih Pengelasan GTAW Standar Industri. Kemudian penelitian ini dilakukan untuk mengevaluasi efektivitas pelaksanaan pelatihan Pengelasan GTAW Standar Industri. Penelitian ini menggunakan Model Evaluasi Kirkpatrick pada tingkat reaksi dan pembelajaran. Kuesioner dan wawancara dipilih untuk mengumpulkan data. Data dianalisis dengan menggunakan teknik deskriptif, uji-t, dan analisis data kualitatif dengan NVivo. Temuan menunjukkan bahwa skor kepuasan peserta pada tingkat reaksi dan pembelajaran sangat tinggi. Penilaian peserta pada tingkat reaksi dilihat dari tiga sudut pandang yaitu reaksi instruktur, reaksi terhadap penyampaian pelatihan, dan reaksi terhadap lingkungan pelatihan. Penilaian peserta pada tingkat pembelajaran dilihat dari dampak pembelajaran dan pelatihan pengetahuan. Pelatihan tersebut membuktikan bahwa program ini berhasil mengembangkan keterampilan peserta pelatihan. Hal ini juga menunjukkan bahwa program tersebut telah berjalan efektif. Namun, produk akhir dari peserta pelatihan tidak memenuhi standar industri. Dalam mengoptimalkan pelatihan ini memerlukan tambahan waktu dan standarisasi kemampuan peserta dengan menggunakan portofolio dari calon peserta pelatihan.
Pengembangan Layout 3D Rest Area dan Wisata Alam Desa Pagersari Berbasis Country Care untuk Meningkatkan Daya Tarik Wisatawan
Authors: Sugandi R. Machmud , Al Ansyorie Mohammad Musthofa , Aris Ichwanto Muhammad , Suwarno Eko , Mega Maharani Nur Ira , Novianti Novianti
Published: September 2024

The location of Pagersari Village, which is in the highlands, makes it have a lot of potential natural resources that can be developed. This is also supported by the Pagersari Village area, which is mostly forest and very fertile land. Therefore, the development of the potential of Pagersari Village to be used as a rest area and nature tourism requires an exciting concept so that the existing potential can attract tourists. One idea that can be developed is to build a rest area and nature tourism in Pagersari Village using a country care basis. In other words, the development of the existing village potential is carried out by prioritizing the concept of caring for the village environment. In this 3D layout concept, tourist spots that can be developed are tourist spots related to nature or the environment of Pagersari Village itself. The natural tourist spot in question can be developed in the rest area, which will also be realized to increase the attractiveness of visitors, which will certainly be related to the welfare of the surrounding community.
Lokasi Desa Pagersari yang terletak di dataran tinggi membuatnya memiliki banyak potensi sumber daya alam yang dapat dikembangkan. Hal ini juga didukung oleh wilayah Desa Pagersari yang sebagian besar berupa hutan dan juga tanah yang sangat subur. Maka dari itu, pengembangan potensi Desa Pagersari untuk dijadikan rest area dan wisata alam memerlukan adanya suatu konsep yang menarik sehingga potensi-potensi yang ada ini dapat menarik minat wisatawan. Salah satu konsep yang dapat dikembangkan adalah membangun rest area dan wisata alam pada Desa Pagersari dengan menggunakan basis country care. Dengan kata lain, pengembangan potensi desa yang sudah ada dilakukan dengan mengedepankan konsep peduli lingkungan desa. Pada konsep layout 3D ini, spot-spot wisata yang dapat dikembangkan adalah spot wisata yang berkaitan dengan alam atau lingkungan Desa Pagersari sendiri. Spot wisata alam yang dimaksud dapat dikembangkan pada rest area yang nantinya juga akan direalisasikan untuk meningkatkan daya tarik pengunjung yang pastinya akan berkaitan dengan kesejahteraan masyarakat sekitar.
A clinicoepidemiological study of chronic and recurrent leg ulcers
Authors: Wilson Anushka , Muppalla Bandhavi , Koneru Yamini Vijaya Sai , Dangi Priyanshi , Nadkarni Nitin , Patil Sharmila , Godse Kiran Vasant
Published: September 2024

Chronic leg ulcers (CLU) are chronic diseases, which are associated with significant morbidity and exert health burden on the patients. Varied heterogeneity in the type of aetiology, risk factors, and comorbidities have been observed in individuals with CLU.

Aprospective, cross-sectional, observational type of descriptive study carried out in the department of Dermatology, Venereology and Leprosy at a tertiary care teaching hospital in Navi Mumbai. Adult patients with clinical evidence of chronic leg ulcer were enrolled. Patient’s quality of life (QOL) was assessed by Dermatology Life Quality Index (DLQI) scale.

The mean age of the sample was 42.07 ± 9.3. The male to female ratio was 1.38:1. The most common occupation encountered was farmers (35%), followed by housewives (13%), shopkeepers (8%), cook & students (7%). Around 76 % of the patients of leg ulcer were having associated co-morbidities among which Diabetes was commonly encountered. The most common etiological diagnosis were venous ulcers (31%), followed by diabetic ulcer (26%), trophic ulcers (25%), traumatic ulcer (7%), leucocytoclastic vasculitis (7%), arterial ulcer and pyoderma gangrenosum (4%). The leg ulcer had adversely impacted the QOL ranging from moderately to extremely large with majority (80%) of the patients having very large impact on QOL.

Chronic leg ulcers are long term conditions with varying aetiologies and risk factors. They are associated with significant impairment of patient’s quality of life.
Stylized Facts on the Quality of Banking Regulation in Latin America and the Caribbean
Authors: Celis Carolina , Galindo Arturo , Rojas-Suárez Liliana
Published: September 2024

This report presents findings from a comprehensive survey of 18 central banks and banking supervisor authorities in Latin America and the Caribbean, including major economies like Argentina, Brazil, Chile, Colombia, and Mexico. The survey aimed to assess the adoption of the Basel III standards across the region and revealed significant diversity in regulatory capital frameworks. Notably, while 75 percent of respondent countries have adopted Basel III for some financial intermediaries, 44 percent still maintain hybrid systems allowing for Basel I or II standards. These results highlight the region's varied approach to financial regulation, pointing to both progress in adopting international standards and the persistence of legacy regulatory regimes. The detailed findings and constructed indexes provide valuable insights into the state of financial regulation in the region, reflecting a landscape of both convergence and divergence in banking supervision practices.
The Impact of Labor Intermediation Training in High Informality Contexts. Evidence from Paraguay
Authors: Campos Nicolás , Chalup Miguel , Mitnik Oscar A. , Urquidi Manuel
Published: September 2024

We provide quasi-experimental estimates of the impact on formal employment of a labor intermediation and training program offered in Paraguay. The Program to Support Labor Insertion (PAIL for its Spanish name) revamped training program design in the country by offering courses aligned with the needs of the private sector, enhancing non-cognitive skills, and combining practical work within companies with classroom training. We combine administrative records which contain detailed information on the employment history and characteristics of all formal workers in Paraguay with an empirical strategy based on extensions of difference-in-differences models and synthetic difference-in-differences. We find that the probability of obtaining formal employment for women and men increases by 11 percentage points. Even two years after participating, the program has a lasting impact on women, an aspect not observed for men. Additionally, we find that the program's impact is positive only in the metropolitan area of Asunción; the program is less effective in areas far from the urban center, especially for men. The observed results suggest that supply-side interventions are ineffective if no formal jobs are available for the beneficiaries.
Artful Diffractions
Authors: Flint Maureen , Wang Yixuan
Published: September 2024

In this article, we explore the potential of artmaking as a reflexive practice, grounded in the work of feminist new materialist philosophers Karen Barad and Donna Haraway. Barad and Haraway’s philosophies emphasize our entanglement with more-than-human materialities and forces and consider our ethical responsibility to the world. We explore our own artful practices such as collaging, drawing, mapping, and creative writing as reflexive practices that help us consider who we are in relation to our research. These examples provide a grounded description for novice and veteran researchers alike who might be interested in using artful reflexive practices in their own work.
Assessing Flow, Sediment, and Salinity Patterns in Tidal-Affected Meandering Rivers: Insights from Kali Wonokromo
Authors: Patriadi Andi , Pattiraja Agustinus Haryanto , Sukmara Riyan Benny , Wahab Muhammad Fajrin
Published: September 2024

This study investigates the interplay between tidal influences and river meandering dynamics in the Wonokromo River, focusing on the alterations in flow patterns, sediment transport, and salinity levels. The research was driven by the need to understand how tidal phases and river meandering affect the geomorphological and ecological characteristics of tidal-affected meandering rivers. Field measurements were conducted across three strategic river cross-sections, employing instruments such as a TH-02 series current meter for flow velocity and a DH-48 type sampler for suspended sediment collection. Salinity was gauged using a Constant WT61 salinometer. Results indicated significant variability in flow velocity, suspended sediment concentration (SSC), and salinity across different river sections and tidal conditions. Notably, river bends showed pronounced asymmetries in flow velocity and SSC, intensified by tidal influences, with higher salinity observed closer to the estuary. The study found that mangrove vegetation along the riverbanks is crucial in stabilizing these parameters, mitigating typical erosion and deposition processes in other meandering rivers. These findings suggest that tidal phases significantly modulate river dynamics, further influenced by local geomorphological features such as bends and vegetation. For future research, exploring the long-term impacts of these dynamics on riverine ecosystems and the potential implications for river management practices, particularly in the context of climate change and human interventions is recommended. This research contributes to the broader understanding of estuarine and coastal river systems, offering insights into sustainable management and conservation strategies.
Aprendizagem autoral no contexto das TDICS: análise de uma Plataforma Educacional
Authors: Matoso Lara Gabriela , Giordani Estela Maris
Published: September 2024

O artigo problematiza a aprendizagem autoral no contexto de uma Plataforma Educacional (PE). O objetivo da pesquisa é analisar se, na visão de profissionais que atuam na área da Educação, as ferramentas, que integram a PE investigada, possibilitam interação, usabilidade, engajamento  e comprometimento entre os sujeitos, promovendo a autoralidade do aprendiz. É fundamental entender que o processo de aprendizagem é orientado pelas habilidades, conhecimentos e atitudes de cada indivíduo (Brasil, 2018), sendo papel da educação potencializar esse conjunto de competências. Isso é possível através do educar pela/para a pesquisa, no qual, dentre seus princípios, estão a autoralidade, autenticidade e protagonismo do aprendiz, garantidos no paradigma da aprendizagem (Demo, 2011; 2018). A pesquisa desenvolvida é de abordagem qualitativa, caracterizada como exploratória de campo. A coleta de dados foi realizada por meio de entrevista semiestruturada, com doze  profissionais da educação, denominados Especialistas. Os resultados demonstraram que, na compreensão dos Especialistas, a PE avaliada tem potencial para transformar as práticas pedagógicas, possibilitando a autoralidade dos aprendizes e o protagonismo responsável, em suas aprendizagens. Pretende-se, com a pesquisa, contribuir para as discussões a respeito da aprendizagem autoral, especialmente na sua relação com as tecnologias. Ainda pode fornecer parâmetros de análise a Plataformas Educacionais a serem implementadas em sistemas públicos e privados de educação, seguindo a Política Nacional de Educação Digital (PNED) (Brasil, 2023).
Origin of the mineralizing fluids involved in the formation of the scheelite skarn in the Beleleita area (Edough NE, Algeria): Fluid inclusion and stable S, O and C isotope study
Authors: Lekoui Abdelmalek , Laouar Rabah , Aissa Djamel Eddine , Boyce Adrian Joseph
Published: September 2024

This study investigates the first stable S-, O- and C-isotopes data on the Beleleita scheelite skarn deposit to assess the origin of the mineralising fluids involved in the formation of the skarn and related W-As-Bi-(Au) mineralisation. Two skarn bodies are embedded within the Neoproterozoic gneisses, south of the Edough metamorphic complex, NE Algeria. They show subparallel, discontinuous slabs (F1 and F2) approximately 700 m long and 10 m wide, striking NE–SW. The slabs were cross-cut by bore-hole drilling at a depth of 130 m during ORGM (Office de Recherche Géologique et Minière) mining exploration in the 1980s. Textural observations reveal two main cycles. Cycle I displays early classical zoned skarn assemblage, including clinopyroxene, garnet, plagioclase, pyrite, pyrrhotite, and chalcopyrite. Cycle II shows late lithiniferous and fluorinated skarn assemblage that corresponds to greisenised secant skarns, with fluorite, scheelite, lollingite, allanite, zinnwaldite series, sphene, wolframite, arsenopyrite, native bismuth, and quartz. Stable O-isotope analyses were carried out on both whole-rock samples and clinopyroxene, quartz, and garnet mineral separates, whereas C- and O-isotope analyses were conducted on gangue calcite. S-isotopes were carried out on sulphides (pyrite, chalcopyrite, lollingite and pyrrhotite). All the results display relatively narrow ranges (δ18OSMOW varying from +8.4 to +9.9‰; δ13CPDB ranging between -6.9 to -4.2‰ and δ34SCDT between -0.3to +5.3‰), indicating a homogeneous source of fluids with significantmagmatic signatures that contribute to skarn formation and ore deposition.Accordingly, the involved mineralising fluids most likely originated from theI-type magmatic event that prevailed during Burdigalian times in theEdough massif, similar to many other ore deposits in the area. Moreover,previous fluid inclusion studies carried out on fluorite, scheelite, and quartz of Cycle II show that the ores were deposited from hot (Th = 500°-520°C), highly saline magmatic fluids under low pressure (0.5–0.6 kb), and this complies well with the present stable isotope data.
Note sur une méthode de biopsie hépatique chez le jeune dromadaire
Authors: Cherrier Renaud , Sumburo M. , Faye Bernard
Published: September 2024

Une technique de biopsie hépatique réalisée sur des chamelons âgés d'environ deux ans est décrite, ainsi que les soins et les risques post-opératoires. La méthode proposée a permis de prélever de 100 à 500 mg de tissu frais dans 75 p. 100 des cas. Ce pourcentage peut s'améliorer quand l'opérateur est bien entraîné à ce type d'intervention.
Influencia de las variaciones climáticas en las infecciones parasitarias de la sierra Scomberomorus sierra (Jordan et Starks) en la Bahía de Acapulco, Guerrero, México
Authors: Santos-Bustos Nataly Graciela , Violante-González Juan , Villalba-Vásquez Princessa Jhossabeth , Salas-Villalobos Shirley Stephany , Gallegos-Navarro Yesenia , Díaz-Gallegos Aldo , Valencia-Cayetano Carlos , Carpio-Hernández Dolores Itzel
Published: September 2024

Los parásitos son considerados como componentes invisibles de los ecosistemas acuáticos y terrestres, los cuales tienen una gran importancia en la dinámica y el flujo de energía dentro de las redes tróficas. Existen pocos estudios enfocados a determinar los posibles efectos que pueden tener algunos fenómenos naturales oceanográficos, tales como El Niño-Oscilación del Sur (ENOS), sobre la estructuración de las comunidades de parásitos de peces marinos. Durante un periodo de nueve años (marzo 2011 a mayo 2019) se examinaron un total de 391 ejemplares de Scomberomorus sierra en la bahía de Acapulco, Guerrero. El objetivo del estudio fue examinar los efectos de eventos climáticos anómalos como La Niña y El Niño sobre las comunidades de parásitos de este pez marino. En el presente estudio se identificaron un total de 20 especies de parásitos metazoarios (15 helmintos y cinco crustáceos). Las comunidades se caracterizaron por una alta dominancia de ectoparásitos, los cuales representaron el 80 % del total de parásitos recolectados. Se registraron cambios importantes en la estructura y composición de especies de las comunidades de parásitos durante las fases fría y cálida del ENOS. Infecciones de algunos parásitos intestinales fueron más frecuentes durante la fase fría, en tanto que durante la cálida fueron más frecuentes e intensas las infecciones por copépodos ectoparásitos. Las variaciones en los niveles de infección de algunas especies de ecto y endoparásitos generaron cambios importantes en la estructura de las comunidades de parásitos de S. sierra en la bahía de Acapulco, durante la ocurrencia de estos eventos climáticos atípicos.
Unveiling Ecological Footprints in BRICS+ Nations: Analyzing Natural Resource Exploration, Renewable Energy Consumption, and Economic Growth
Authors: Riaz Mahmud Hasan , Alam Montasir , Ali Ayub , Raihan Asif , Ahmed Zobayer
Published: September 2024

This study examines the impact of natural resource exploitation, renewable energy consumption, economic growth, biocapacity, and information and communication technologies (ICT) on the ecological footprint of BRICS+ nations using panel data from 1991 to 2019. Advanced econometric techniques, including FMOLS, DOLS, CCR, and GMM, reveal significant findings relevant to sustainability-focused policymakers. The results show that natural resource exploitation significantly contributes to environmental degradation, while renewable energy consumption mitigates these effects to varying degrees across the studied countries. Although economic growth is crucial, it worsens ecological footprints unless accompanied by sustainable practices. ICT and biocapacity emerge as key factors in promoting environmental sustainability, with ICT enhancing innovation and efficiency to reduce ecological impacts. The study emphasizes the need for tailored policy frameworks that align economic growth with environmental sustainability, advocating for the adoption of renewable energy, eco-innovations, and green finance initiatives suited to the unique conditions of BRICS+ nations. The findings aim to guide policymakers in advancing sustainable development and mitigating the adverse environmental impacts of rapid industrialization and urbanization, while contributing to the global discourse on achieving a balance between economic growth and ecological well-being in developing countries.
IgG Antibody Titers against Ascaris lumbricoides, Strongyloides Stercolaris, and Toxocara canis in Venezuelan Patients with Asthma or COPD
Authors: De Sanctis Juan Bautista , Moreno Dolores , Larocca Nancy , Garmendia Jenny Valetina
Published: September 2024

It has been suggested that parasitic infections, common in Latin American populations, may amplify the inflammatory response of the airways. There are several reports in atopic and asthmatic patients, but few reports of parasitic infection in COPD patients. The study aimed to determine the prevalence of parasitic infections in COPD patients as compared to atopic and asthmatic patients attending the outpatient clinics of the Institute of Immunology and the pneumonology service of the University hospital. A case-control study was conducted: 100 patients with bronchial asthma, 100 patients with COPD, 100 individuals with atopy without respiratory symptoms, and 100 healthy individuals. Serum specific IgG antibody against the parasites Ascaris lumbricoides (Al), Strongyloides stercolaris (Ss) and Toxocara canis (Tc) were measured: by ELISA. IgE levels were used as an indirect indicator of atopy. Positive IgG for Al was observed in all groups, predominantly in atopic cohort, Ss positiveness was recorded only in 4 COPD patients and Tc positiveness was observed in all groups except in controls. There are significant correlations between the values of Al and IgE in controls, atopic and asthmatic patients not with COPD. No correlation was found for Tc. IgE levels and FEV1 correlate only in atopic and asthmatic patients. Parasitic infections are common in atopic patients and in moderate and severe asthmatic and COPD patients. Anti-inflammatory treatment may be responsible for the increase frequency of infection.
Cdk1 Deficiency Extends the Post-natal Window of Cardiomyocyte Proliferation and Restores Cardiac Function After Myocardial Infarction
Authors: Mahiny Donya , Hauck Ludger , Premsingh Benny , Grothe Daniela , Billia Filio
Published: September 2024

Cyclin-dependent kinase 1 (Cdk1) is a master regulator of the G2-M transition between DNA replication and cell division. This study investigates the regulation of cardiomyocyte (CM) proliferation during the early neonatal period and following ischemic injury in adult mice. We analyzed cell cycle dynamics with the assessment of DNA synthesis, and cytokinesis in murine hearts during the first 15 days after birth. A distinct proliferative block was observed at 1 day, followed by a second wave of DNA synthesis at 4 days, leading to CM binucleation (CMBN) by day 5. Genome-wide mRNA profiling revealed differential expression of cell cycle regulatory genes during this period, with a downregulation of factors involved in cell division and mitosis. The loss of Cdk1 impaired CMBN but extended the neonatal CM proliferation window until day 10 post-birth. In adult hearts, cardiac-specific ablation of Cdk1 triggered CM proliferation post-myocardial infarction (MI) in specific zones, driven by the activation of EGFR1 signaling and suppression of the anti-proliferative p38 and p53 signalling. This was accompanied by restoration of fractional shortening, mitochondrial function, and decreased reactive oxygen species. Additionally, cardiac hypertrophy was mitigated, and survival rates post-MI were increased in Cdk1 knockout mice. These findings reveal a novel role of Cdk1 in regulating cell cycle exit and re-entry in differentiated CM and offer insights into potential strategies for cardiac repair.
Bloodmeal, and Selected Indigenous Raw Materials as Cost-Effective Feeds for Milkfish Aquaculture
Authors: Macusi Edison D. , Jimenez John Edward M. , Vender Ethel Kate E. , Bong-as Henzel P. , Macusi Erna S. , Andam Michael B.
Published: September 2024

This study is based on the clamor of fish farmers for a cheaper fish feed alternative to current commercial feeds. At present, 70-80% of aquaculture costs come from feed cost, keeping farmers' income below their expectation. This study aimed to find alternative feeds or supplementary feeds that can perform similarly to the existing commercial feeds thereby reducing farmer expense and cutting down their cost of production and increasing their profitability. To do that, various indigenous raw materials were selected based on an earlier study which could result in a better feed alternative. The formulations for the study included four grow-out treatments mainly: control (commercial feed), taro+control (powdered Colocasia esculenta+commercial fish feed), bloodmeal (formulated fish feed based on bloodmeal), banana stem+control (chopped banana stem+commercial fish feed). A total of 12,000 fingerlings initially weighing 16 g (TL=10 cm) individually were assigned randomly to the four treatments (3000 in each cage measuring 6 x 6 m). Week zero was used for acclimation period, and fingerlings were fed with commercial fish feed. Then the first week used the treatment diets which were adjusted based on average body weight and sampled weekly (ABW=23g; TL=11 cm). The result of the study first showed significant differences between treatments on their fourth week in terms of the diet treatments (MS=0.103, df=3, F=4.12, P=0.0076) with the control and the taro+control showing no difference in terms of weight and length (TC vs CF; P=0.4524). But during the fifth week, the bloodmeal treatment showed increased weight and length for fish sampled (MS=0.109, df=3, F=4.10, P=0.0076) and provided similar weight and length as those from the taro+control and the control (TC vs CF; P=0.3721 and BM vs CF; P=0.0587). During the succeeding weeks, there were no significant differences between feed treatments from 6th weeks onwards, whether with weight [MS=0.130, df=3, F=1.14, P=0.333] or total length of the treatments [MS=0.008, df=3, F=0.40, P=0.75]. The FCR ratio showed taro (1.81) as lowest, followed by blood meal (2.09), and control (2.24) and then banana stem (2.84). In terms of weight gain, fish from the bloodmeal treatment gained the highest (236.97 g), followed by control (223.33 g), then taro (217.67 g) and banana stem (196.99 g). As a result, we find that the tested treatments such as bloodmeal formulated feed, taro+commercial feed, and banana stem+commercial feed can serve as an alternative to purely commercial fish feeds. Cost-benefit analysis showed that a better gross profit margin of 42% and cost-benefit ratio of 1.37 was observed in taro+commercial feed diet.
A Brief Review on the Cell Culture History: From Harrison to Organs-on-a-Chip
Authors: Moro Lincoln Gozzi , Guarnier Lucas Pires , Azevedo Maurício Fogaça , Fracasso Julia Amanda Rodrigues , Lucio Marco Aurélio , Castro Mateus Vidigal de , Dias Marlon Lemos , Lívero Francislaine Aparecida dos Reis , Ribeiro-Paes João Tadeu
Published: September 2024

This comprehensive overview of the historical milestones in cell culture underscores key breakthroughs that have shaped the field over time. It begins with Wilhelm Roux's seminal experiments in the 1880s, followed by the pioneering efforts of Ross Granville Harrison, who initiated groundbreaking experiments that fundamentally shaped the landscape of cell culture in the early 20th century. Carrel's influential contributions, notably the immortalization of chicken heart cells, mark a significant advancement in cell culture techniques. Holtfreter, Moscona, and Leighton introduced innovations such as 3D cell culture, laying the groundwork for further exploration. The advent of induced pluripotent stem cells by Takahashi and Yamanaka in 2006 was revolutionary, enabling the reprogramming of differentiated cells into a pluripotent state. Since then, recent innovations include spheroids, organoids, and organ-on-a-chip technologies, aiming to mimic the structure and function of tissues and organs in vitro, pushing the boundaries of biological modeling and disease understanding. In this review, we overview the history of cell culture shedding light on the main discoveries, pitfalls and hurdles that were overcome during the transition from 2D to 3D cell culture techniques. Finally, we discussed the future directions for cell culture research that may accelerate the development of more effective and personalized treatments.
The Seeds Combining Pyrrolidine Controlling the Framework Al Distribution of FER Zeolites to Enhance Its Performance in the Skeletal Isomerization of n-Butene
Authors: Fan Jinlong , Zhu Xuedong , Yang Fan , Xu Yarong , Chen Lantian
Published: September 2024

FER zeolites has a unique framework structure and acid distribution, and is widely studied as a catalyst for reactions such as n-butene skeletal isomerization and dimethyl ether carbonylation. The BAS located in the 10-MR of FER zeolites serves as the active site for the isomerization reaction of n-butene skeletal to produce isobutene. In contrast, the acid site found in the 8-MR is prone to side reactions, such as polymerization and cracking, which can adversely affect the selectivity of isobutene. This study prepared five types of FER zeolites using different method, inducing Na-form or H-form crystal seeds without organic structure directing agents (OSDAS), using pyridine alone as a template, and combining the seeds with pyridine. The differences in the structure and acid distribution of the five zeolites were investigated, as well as their catalytic performance for the the skeletal isomerization of n-Butene. Experiments and characterization results showed that under hydrothermal synthesis conditions, the FER-PY+SH zeolites synthesized by using both H-form zeolites seeds and pyridine exhibited the highest aluminum concentrations at T1 and T3 positions, along with the greatest BAS of 10-MR. This unique composition contributed to the highest selectivity of isobutene. The FER-PY+SH catalyst was continuously used for 720 hours at 350 ℃, 0.1 MPa, and n-butene mass space velocity of 2.0 h-1 for three recycles on 2160 h. During this period, the conversion of n-butene over 39%, while the selectivity of isobutene exceeded 95%.The catalyst exhibited excellent stability and activity.
Basic Guide for Approaching Drug Delivery with Extracellular Vesicles
Authors: Brezgin Sergey , Danilik Oleg , Yudaeva Alexandra , Kachanov Artyom , Kostyusheva Anastasiya , Karandashov Ivan , Ponomareva Natalia , Zamyatnin Jr. Andrey A , Parodi Alessandro , Chulanov Vladimir , Kostyushev Dmitry
Published: September 2024

Extracellular vesicles (EVs) are natural carriers of biomolecules that play a crucial role in cell-to-cell communication and tissue homeostasis under normal and pathological conditions, including inflammatory diseases and cancer. Since the discovery of the pro-regenerative and immune-modulating properties of EVs, EV-based therapeutics have entered clinical trials for conditions such as myocardial infarction and autoimmune diseases, among others. Due to their unique advantages—such as superior bioavailability, substantial packaging capacity, and the ability to traverse biological barriers—EVs are regarded as a promising platform for targeted drug delivery. However, achieving sufficient accumulation of therapeutic agents at the target site necessitates a larger quantity of EVs per dose compared to using EVs as standalone drugs. This challenge can be addressed by administering larger doses of EVs, increasing the drug dosage per administration, or enhancing the selective accumulation of EVs at target cells. In this review, we will discuss methods to improve the isolation and purification of EVs, approaches to enhance cargo packaging—including proteins, RNAs, and small-molecule drugs—and technologies for displaying targeting ligands on the surface of EVs to facilitate improved targeting. Ultimately, this guide can be applied to the development of novel classes of EV-based therapeutics and to overcoming existing technological challenges.
Response of Native and Non-native to Subarctic Plant Species to Continuous Illumination by Natural and Artificial Light
Authors: Shibaeva Tatjana G. , Sherudilo Elena G. , Rubaeva Alexandra A. , Shmakova Natalya Yu. , Titov Alexander F.
Published: September 2024

The study addressed the following questions: How does continuous lighting (CL) im-pact plant physiology, photosynthetic and stress response? Does the impact of CL de-pend on the source of light and other environmental factors (natural vs artificial)? Do responses to CL differ for native and non-native plant species in the Subarctic and, if differences exist, what physiological reasons might they be associated with? Experi-ments were conducted with three native to Subarctic (Geranium sylvaticum L., Geum rivale L., Potentilla erecta (L.) Raeusch.) and three non-native plant species (Geranium himalayense Klotzsch, Geum coccineum Sibth. & Sm., Potentilla atrosanguinea Loddiges ex D. Don) introduced in Polar-Alpine Botanic Garden (KPABG, 67°38′N). Obtained re-sults have shown that plants lack specific mechanisms of tolerance to CL. Protective responses are non-specific and induced by developing photo-oxidative stress. In cli-mate chambers under constant environmental conditions artificial CL causes leaf inju-ries due to oxidative stress, the main cause of which is circadian asynchrony. In nature plants are not photodamaged during the polar day as endogenous rhythms are main-tained due to daily fluctuations of several environmental factors (light intensity, spec-tral distribution, temperature and air humidity). The obtained data show that among possible non-specific protective mechanisms, plants use flavonoids to neutralize excess ROS generated under CL. In local subarctic plants, their photoprotective role is signif-icantly higher than in non-native introduced plant species.
Neutrophils Respond Selectively to Physical Cues: Roughness Modulates Its Granule Release, and NETosis
Authors: Elangovan Gayathiri , Cameron Andrew , Fernandes Daniel , Basu Souptik , De Mello Neto Joao Martins , Jiang Anna Peishan , Reher Peter , Hamlet Stephen , Figueredo Carlos Marcelo S
Published: September 2024

This study evaluated the impact of rough, smooth, and very smooth Titanium (Ti) and zirconia (Zr) surfaces on the neutrophil expression of elastase (NE), matrix metalloproteinase (MMP)-8, MMP-9 and extracellular traps (NETs). Ti and Zr disks with a diameter of 10mm and a thickness of 1.5mm were processed using diamond-impregnated polishing burs and paste to generate rough (R; Ra= > 3µm), smooth (S; Ra= ≥1 to 1.5µm) and very smooth (VS; Ra= <0.1µm) surfaces. Peripheral neutrophils from Wistar rats were cultured on these surfaces and the culture supernatants subsequently analyzed for the presence of NE, MMP-8, and MMP-9 by ELISA, while NETs formation was demonstrated immunohistochemically by staining neutrophils with CD16b and DNA with DAPI. Overall, the expression of NE and MMP-8 were significantly higher from neutrophils cultured on both on Ti and Zr rough surfaces compared to the very smooth surface (R>S>VS) after 2h and 4h of culture. The expression of MMP-9 also increased with culture time however no significant surface effects on expression were observed. Similarly, rough Ti and Zr surfaces (R & S) also showed significantly larger NETs formation compared to the very smooth surface (VS) after 4h and 8h culture. Our findings suggest that increasing surface roughness on Ti and Zr triggers higher secretion of NE, MMP-8 and NETs formation.
Platelet Function, Platelet Size and Content of Reticulated Platelets. Interactions in Patients Receiving Dual Antiplatelet Therapy
Authors: Bodrova Valeria V. , Shustova Olga N. , Golubeva Nina V. , Alieva Amina K. , Vlodzyanovsky Vladislav V. , Pevzner Dmitry V. , Mazurov Alexey V.
Published: September 2024

Increased platelet activity is a risk factor of thrombotic events in cardiovascular patients. We studied the relationship between platelet function, platelet size and content of reticulated platelets (RP) in patients with coronary heart disease (CHD, n = 55) and acute coronary syndrome (ACS, n = 95) receiving acetylsalicylic acid + clopidogrel or ticagrelor respectively. Control group consisted of patients with risk factors for CHD, but no CHD/ACS and free of antiplatelet drugs (n = 66). Platelet function was evaluated by exposure of activated glycoprotein (GP) IIb-IIIa and P-selectin. In the control group, platelets were activated by TRAP (Thrombin Receptor Activating Peptide) 10 µM, ADP 20, 5, 2.5 µM and in CHD/ACS groups, by TRAP 10 µM, ADP 20, 5 µM (+/- epinephrine 20 µM). Platelet size was assessed by mean volume, % large forms and forward scattering. RP were stained by thiazole orange. In the control group activated GP IIb-IIIa and P-selectin correlated with platelet size and RP content after platelet activation by all agonists. Despite the decrease of platelet activity by antiplatelet drugs, most correlations (primarily for activated GP IIb-IIIa) were preserved in CHD/ACS patients. In conclusion, increased platelet size and RP content are associated with increased platelet activity and reduced efficacy of antiplatelet therapy.
Waste Management and Environmental Health Impact – Sustainable Laboratory Medicine as Mitigating Response
Authors: Nwobi Nnenna Linda , Anetor Gloria Oiyahumen , Nwobi Joseph Chigbogu , Igharo Godwin Osaretin , Adeyemi Anuoluwapo Victor , Anetor John Ibhagbemien
Published: September 2024

Laboratories generate various forms of wastes which contribute to a complex network of profound and multifaceted environmental challenges and associated public health issues if not properly managed.Traditional laboratory practices particularly in developing countries, often lead to excessive waste generation, chemical contamination and resource depletion, coupled with inefficient waste management which poses risks to both ecosystem and public health. As the environmental impact of laboratory waste escalates, the need for effective waste management becomes increasingly important and urgent for safeguarding environmental health. This review examines the impact of laboratory waste management practices on environmental health and explores sustainable laboratory medicine as a mitigating response. Sustainable laboratory medicine adopts practices such as green laboratory principles involving waste minimization, resource conservation, the use of eco-friendly materials as well as energy-efficient protocols and innovative waste segregation and treatment technologies. This approach complies with regulatory standards as well as emphasizes proactive orientation towards environmental stewardship, operational efficiency and innovation while maintaining scientific integrity. Sustainable laboratory medicine appears to be a crucial framework that mitigates the environmental health impacts associated with laboratory-derived waste. This review advocates for a paradigm shift towards sustainable laboratory operations and emphasizes the need for comprehensive training, institutional commitment, and regulatory support.