bluuebunny/crossref_metadata_2025 huggingface dataset:
Granular Cell Tumour of the Breast Cancer: Challenging Clinical and Radiological Mimic of Cancer
Authors: Abolins Arnis , Strumfa Ilze , Vanags Andrejs , Trofimovics Genadijs , Gardovskis Janis
Published: July 2012

Granular cell tumours (GCT) are rare soft tissue neoplasms of unclear histogenesis affecting almost any organ or tissues. Approximately 5% of GCT cases are located in the breast. Although granular cell tumour is benign in most instances, it can mimic breast cancer by radiological and clinical traits. Increased awareness of GCT is necessary to avoid misinterpretation as cancer and subsequent overtreatment
Pathological Features of BRCA1/BRCA2 Mutation-Associated Breast Cancer: Implications for Diagnostics and Treatment
Authors: Melbarde-Gorkusa Inga , Strumfa Ilze , Vanags Andrejs , Trofimovics Genadijs , Gardovskis Janis
Published: July 2012

Pathological Features ofBRCA1/BRCA2Mutation-Associated Breast Cancer: Implications for Diagnostics and Treatment

BRCA1andBRCA2gene mutations are responsible for significant hereditary breast cancer burden.BRCA1/2mutation-associated breast cancers (furtherBRCA1orBRCA2cancers) are distinctive not only by family history but also by the biological features of the tumour influencing both diagnostic possibilities and response to different treatment modalities. Distinctive morphology and immunohistochemical phenotype of hereditary breast cancers may help to identify patients who are likely to carry germ line mutations inBRCA1orBRCA2gene. The efficacy of specific treatment options can be predicted as well. Additionally,BRCA1carcinomas have different histopathological manifestations fromBRCA2cancers. The cellular and molecular characteristics ofBRCA1/2breast cancer can explain the clinical data and provide prognostic and predictive information. Here, we discuss the peculiarities of breast cancer inBRCA1/2mutation carriers having significant implications in the diagnostics, surgical approach and overall planning of treatment.
Potential Role of Cytokines in Children with Acute Appendicitis and Acute Mesenteric Lymphadenitis
Authors: Zviedre Astra , Engelis Arnis , Kakar Mohit , Pētersons Aigars
Published: July 2012

Potential Role of Cytokines in Children with Acute Appendicitis and Acute Mesenteric Lymphadenitis

Although, AAP and AML have different etiological factors, clinical symptoms are very much similar but treatment tactics in both the disease differ a lot. In case of AML, treatment is more conservative and does not require hospitalization while in case of AAP immediate hospitalization and maybe further surgery can be mandatory. With the identification of group of cytokines serum inflammatory mediators IL-8, IL-10, IL-12[p70], IL-17, TNF-a and MCP-1, it is believed early and proper diagnosis of AAP in the near future. Research of cytokines-serum inflammatory mediators has opened new opportunities for an early detection and differentiation of these two diseases in children.
Evaluation of Oral Therapeuthical and Surgical Treatment Needs among Retirement Age Population in Different Countries
Authors: Krasta Ingrida , Vidzis Aldis , Brinkmane Anda , Cema Ingrida
Published: July 2012

Evaluation of Oral Therapeuthical and Surgical Treatment Needs among Retirement Age Population in Different Countries

Oral health in connection with quality of life is affected by such functional factors as dental decay and its complications, untreated tooth roots, oral mucosal diseases and inflammations, precancerous diseases, cancers, pain in temporomandibular joints, xerostomia and partially or fully edentulous jaws. It has been noted in literature that among retirement age population the number of remaining teeth has increased and the number of untreated decayed teeth in developed countries for the last 20 years has decreased. Despite this fact the need to improve measures of oral health remains actual in this age group due to increasing prevalence of diagnosed oral diseases and number of extracted teeth and roots. Oral health indicators among retirement age population living in nursing homes in such countries as Canada, USA, UK, Finland, Denmark, Germany, Turkey, Brazil, Australia and Lithuania differ from the same age group indicators among self-dependent old people able to take care of themself. Oral health indicators of nursing homes residents in many countries are significantly worse than oral health indicators of the corresponding age group population. The proposed evaluation data of oral hygiene, periodontal status, DMF-T index, quality of existing and needs of new prosthodontics as well as oral mucosal disorders among retirement age population provides an important insight into therapeutic and surgical treatment provision in different countries.
Management of BRCA1 Mutation Carrier with Breast Cancer
Authors: Melbarde-Gorkusa Inga , Strumfa Ilze , Irmejs Arvids , Abolins Arnis , Miklasevics Edvins , Gardovskis Andris , Subatniece Signe , Trofimovics Genadijs , Gardovskis Janis
Published: July 2012

Management ofBRCA1Mutation Carrier with Breast Cancer

BRCA1gene mutation carriers face high lifetime risk of breast cancer therefore specific diagnostic features should be considered and individualized treatment strategies should be applied. ForBRCA1mutation carriers with already developed breast cancer, extensive surgery - bilateral mastectomy - could be indicated due to the cancer risk in the contralateral breast.
Incidental Primary Non-Hodgkin's Lymphoma of the Prostate in a Patient with Prostate Cancer: A Case Report
Authors: Vjaters Egils , Strumfa Ilze , Vejins Mareks , Abele Andris , Trofimovics Genadijs , Gardovskis Janis
Published: July 2012

Incidental Primary Non-Hodgkin's Lymphoma of the Prostate in a Patient with Prostate Cancer: A Case Report

Primary lymphoma of the prostate is uncommon; it is extremely rare combination of adenocarcinoma and primary Non-Hodgkin's lymphoma of the prostate. We report a case of 69-years-old man who was treated with a radical surgery.
First Liver Transplantation in Latvia for Patient with Primary Sclerosing Cholangitis
Authors: Vilmanis Janis , Ozolins Arturs , Kisis Kaspars , Kovalovs Sergejs , Veiss Andris , Savlovskis Janis , Strike Eva , Gardovskis Janis
Published: July 2012

First Liver Transplantation in Latvia for Patient with Primary Sclerosing Cholangitis

This is the case of first orthotopic liver transplantation (LT) in Latvia. LT was done due to liver cirrhosis caused by primary sclerosing cholangitis (PSC). After LT patient had two complications - stenosis of portal anastamosis and necrosis of common bile duct (CBD), which were successfully solved with stenting of v.porta and reconstructive operation of CBD.
Method of Assessing the Impact of Material Properties on the Propagation of Vibrations Excited with a Single Force Impulse
Authors: Burdzik R. , Stanik Z. , Warczek J.
Published: July 2012

Method of Assessing the Impact of Material Properties on the Propagation of Vibrations Excited with a Single Force Impulse

The article provides a discussion on the studies comprising active experiments conducted on selected structural components, the purpose of which was to analyse the impact of properties of the material used on the dynamic state form. The vibration excitation was achieved by applying a force impulse of a specific value. The studies were conducted on excitations acting perpendicularly to the surface of the component being examined in strictly established geometric arrangements. Consequently, signals were obtained to be subsequently applied for the sake of analysis of the impact of material properties on the manner of the vibration energy propagation in the machine components examined. The signal processing method used was based on application of a time and frequency transformation which enables obtaining the information the signals carry in two domains simultaneously, namely those of time and frequency. The results provided and discussed evidence the applicability of the method described in the analysis of the material impact on dynamic properties of the given structural component.
Wrought Magnesium Alloys ZM21, ZW3 and WE43 Processed by Hydrostatic Extrusion with Back Pressure
Authors: Pachla W. , Mazur A. , Skiba J. , Kulczyk M. , Przybysz S.
Published: July 2012

Wrought Magnesium Alloys ZM21, ZW3 and WE43 Processed by Hydrostatic Extrusion with Back Pressure

Cold hydrostatic extrusion with and without back pressure of commercial ZM21, ZW3 and WE43 magnesium alloys has been performed at originally designed hydrostatic extrusion press operating up to 2000 MPa with back pressure up to 700 MPa. Alloys were cold extruded in one pass into rods between 5 and 9 mm in the outer diameter with product velocities between 1 and 10 m/min and extrusion ratios above 2. Application of back pressure extended formability of all magnesium alloys. It was due to hydrostatic pressure superimposed on the extruded product what inhibits the cracks generation and propagation. Cold deformation restrained the grain growth and softening processes while severe deformation in one pass increased grain refinement and density of internal defects. Ultimate tensile strength ranging from 370 MPa (ZM21) through 400 MPa (ZW3) up to 410 MPa (WE43), with respective yield stresses from 270 MPa through 300 MPa up to 350 MPa and the respective elongation from 13% through 12% to 7% were obtained in extruded rods, which are the best reported data in literature up to this day. Wrought magnesium alloys after hydrostatic extrusion can serve as semi-products for structures that call for high strength, for example as biodegradable implants or fastening components in form of bolts, rivets, nuts, pins, joints, etc.
In the Jewelry, Brazing and Laser Welding with Parameter Optimization
Authors: Kallek M. , Ataoglu S. , Yagci Y. , Bozkurt H. , Gulluoglu A.
Published: July 2012

In the Jewelry, Brazing and Laser Welding with Parameter Optimization

In this study, brazing and laser welding were experimentally compared for 9, 14 and 18 carat gold alloys in different color. The gold alloys were joined by laser welding and brazing and the microstructure, hardness, chemical composition, color of the weld joints were investigated. According to study, in laser welding, voltage, pulse duration, pulse diameter and frequency are the most important welding parameters that influence the quality of joints. Welded joints are characterized using three geometric parameters: penetration depth, width of heat affected zone and underfill defects. Finally, optimum laser welding parameters of different color of 9, 14 and 18 carat gold alloys were determined. It is observed that laser welding has numerous advantages compared to the other joining techniques used in the jewelry industry.
Kinetic Studies of Isothermal Decomposition of Unirradiated and Γ-Irradiated Gallium Acetylacetonate: New Route for Synthesis of Gallium Oxide Nanoparticles
Authors: Mahfouz R.M. , Al-Khamis Kh.M. , Siddiqui M.R.H. , Al-Hokbany N.S. , Warad I , Al-Andis N.M.
Published: July 2012

Isothermal decomposition of unirradiated and γ-irradiated gallium acetylacetonate Ga(acac)3 with 103 kGy total γ-ray dose was carried out in static air. The isothermal operating temperatures were 160, 170, 180 and 190°C. The kinetics of decomposition were followed using both model-fitting and model-free approaches. The results of model fitting application on the investigated data showed that the decomposition behaviour was best described by phase-boundary controlled reaction (R2). Kinetic parameters of the decomposition process were calculated and evaluated. Analysis of the data using model free approach signifies the dependency of Ea on extent of conversion (α). Pre-γ-irradiation of gallium acetylacetonate Ga(acac)3 with 103 kGy total γ-ray dose has almost no effect on the kinetic parameters.
Effect of Storage Containers and Storage Periods on The Seed Quality of French Bean (Phaseolus vulgaris)
Authors: Khalequzzaman KM , Rashid MM , Hasan MA , Reza MA
Published: July 2012

The experiment was conducted in the Laboratory, Department of Plant Pathology,  HSTU, Dinajpur during April  July 2010 to know the effect of abiotic and biotic  factors, storage periods and storage containers on the seed quality of French  bean. Tin container showed the highest germination, normal seedlings and  vigour index which were followed by polythene bag, where Gunny bag showed  the lowest germination, normal seedlings and vigour index upto 60 days after  storage. The highest 1000-seed weight, moisture content, abnormal seedlings,  seed rot and incidence of the Fusarium oxysporum were recorded in Gunny bag,  where the lowest of these parameters were recorded in Tin container. Seed  colour of Tin container were more or less same as initial colour, but seeds of  Gunny bag were changed into fade or brown colour at 60 days after storage.  Moisture content, 1000-seed weight, abnormal seedlings, seed rot, fungi  association were increased, but germination and normal seedlings were  decreased with the increase of storage periods. Among the three containers, Tin  container was the best and the Gunny bag was the worst storage containers upto  60 days of storage for French bean seed. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11221 Bangladesh J. Agril. Res. 37(2): 195-205, June 2012
Study on Intercropping Carrot with Groundnut Under Different Row Arrangements
Authors: Mondal MRI , Begum F , Alam MM
Published: July 2012

Burirhat, Rangpur during two consecutive seasons of 2004-05 and 2005-06, respectively, to find out suitable row arrangement of carrot with groundnut for higher yield and economic return. There were six treatments, such as sole groundnut, sole carrot, one row of carrot in between two normal rows of  groundnut, two rows of carrot in between two normal rows of groundnut, two  rows of groundnut alternated with two rows of carrot and three rows of  groundnut alternated with three rows of carrot. Results showed that monoculture  produced the highest yields of individual crops but in intercropping system the  highest groundnut equivalent yield (10.63 t/h and 11.10 t/ha) was obtained from two rows of carrot in between two rows of groundnut. The maximum land  equivalent ratio (1.67 and 1.74), the highest gross return (Tk.212600/ha and  Tk. 248400/ha) and net return (Tk.184881/ha and Tk.211680) were also obtained from the intercropping treatment with two rows carrot in between two normal rows of groundnut. But due to higher cost in this treatment, maximum benefit cost ratio (7.09 and 7.01) was obtained from the intercropping treatment    with one row carrot in between two normal rows of groundnut in both the years. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11223 Bangladesh J. Agril. Res. 37(2): 215-223, June 2012
Management of Organic Manure and Inorganic Fertilizer in The Maize-Mungbean/Dhaincha-T. Aman Rice Cropping Pattern for Increased Crop Production
Authors: Rahman MH , Islam MR , Jahiruddin M , Haque MQ
Published: July 2012

The study was conducted at the On Farm Research Division (OFRD) farm, Rangpur from 2002-2003 to 2004-2005 to see the effect of inorganic fertilizers along with organic manure and mungbean residue on soil properties and yield of crops. For the first crop (maize), there were five treatments. After harvest of maize, mungbean and dhaincha (Sesbania) seeds were sown as per treatments. For T. Aman rice (third crop), each of the treatments (T2 and T3 plots) were subdivided into six, so there were altogether 15 treatments. Integrated use of manure and inorganic fertilizers (IPNS basis) produced comparable seed yield of maize with the chemical fertilizers alone irrespective of moderate or high yield goal basis (MYG or HYG). The highest maize yield of 10.02 t/ha was obtained from the treatment T5, which produced significantly highest yield over all other treatments. During the growing period of mungbean, temperature coupled with rainfall encouraged vegetative growth of mungbean and as a result, pod formation was low. The incorporation of Sesbania biomass and mungbean residue along with inorganic fertilizers for MYG produced identical grain yields of T.Aman rice with the fertilizers alone for HYG. The highest grain yield 4.31 t/ha was found in IPNS dhaincha along with fertilizers for HYG treatment. There was no remarkable change in post harvest soil status during the growing period. It may be concluded that addition of mungbean residues or Sesbania biomass before T. Aman rice may ensure higher crop productivity and sustain soil fertility. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11224 Bangladesh J. Agril. Res. 37(2): 225-234, June 2012
Assessment of Technical Efficiency of Inbred HYV and Hybrid Rice Cultivation at Farm Level
Authors: Salam MA , Siddique MAB , Parvin J
Published: July 2012

This study is very important in the present perspective of rice economy as  comparative economics of inbred HYVs and hybrid rice production was very  scanty. As such, the present study was conducted to examine the relative  productivity, profitability, and comparative technical efficiency of inbred HYVs  and hybrid rice production in some selected areas of Gazipur district. The study  was conducted in four villages in Sadar Upzila under Gazipur district, namely  Kesurita, Martarchar, Harinal, and Samantapur. A total of 80 farmers from the  four villages were interviewed consisting of 40 farmers for hybrid and 40 for  inbred HYVs rice. Data were generated by personal interview using structured  questionnaire through conducting farm level survey. The analysis revealed  that there was no significant difference between farmers practices and  recommended rate of hybrid seed, TSP, and MP. But the farmers used  urea and seed of inbred HYVs significantly higher than recommend  rate. Hybrid farms incurred total cost of Tk. 63377/ha and inbred farms  incurred Tk. 61195/ha, respectively. Net returns obtained from hybrid rice was  Tk. 59,056/ha whereas it was Tk. 42,818/ha for inbred HYVs rice. Average net  return of inbred rice was 38% lower compared to that of hybrid rice.  Benefit cost ratio of inbred and hybrid production was estimated to be  1.93 and 1.70, respectively. The average yield of inbred HYV was 6.03 t/ha and  by product was 4.50 t/ha, while those of hybrid were 7.76 t/ha and 5.50 t/ha,  respectively. The estimates of technical inefficiency implied that education,  farming experience, extension contact, land type, seedling age, and number of  seedlings per hill were the major determinants of inefficiency for both inbred  and hybrid rice growers. The mean technical efficiency was about 80% for  inbred and 86% for hybrid rice producers, respectively, indicating hybrid rice  growers were technically more efficient than inbred growers. Higher-level of  education and more contact with extension agents were found to contribute in  reducing technical inefficiency of both inbred and hybrid rice producers.  Although, inbred and hybrid rice producers faced some problems, but it was  more severe for hybrid DOI: http://dx.doi.org/10.3329/bjar.v37i2.11225 Bangladesh J. Agril. Res. 37(2): 235-250, June 2012
Integrated Nutrient Management for Sustaining Soil Fertility Through Chickpea-Mungbean-T.Aman Cropping Pattern at Madaripur Region
Authors: Quddus MA , Rashid MH , Hossain MA , Naser HM , Mian J Abedin
Published: July 2012

A field experiment was conducted on Chickpea-Mungbean-T.Aman cropping pattern at Pulses Research Sub-Station, Madaripur under Low Ganges River Floodplain Soils (AEZ-12) during 2007-08 and 2008-09 to find out the suitable fertilizer doses for this pattern. Four treatments were set up for each crop. For chickpea and mungbean, the treatments were T1=Recommended fertilizer dose as per FRG, 2005 BARC (N15P18K10S<5Zn0.5B0.5); T2=Soil test based fertilizer dose   (N21P23K30S18Zn2B1.5); T3=Farmers practice (N23P15K8); and T4=Control (without fertilizer). For T.Aman, the treatments were T1= Recommended fertilizer dose as per FRG, BARC (N66P7K12S6Zn1); T2=Soil test based fertilizer dose (N130P14K76S6Zn1.5B1.0); T3=Farmer practice (N90P10K15); and T4=control.   Experimental results revealed that among the treatments the highest seed and stover/straw yields of chickpea (1524 kg/ha and 4049 kg/ha), mungbean (2208 kg/ha and 5121 kg/ha) and T.Aman (5414 kg/ha and 5615 kg/ha) were recorded in treatment T<2. This treatment was significant at 5% level except seed yield of   chickpea in 2008-09. T3 treatment showed significant difference with T4 treatment.  The lowest seed and stover/straw yields of all the crops were recorded in control treatment (T4). After completion of two years pattern cycle, the organic matter, total  nitrogen, phosphorus, sulphur, zinc, and boron were higher in treatment T2. The   economic analysis revealed that the highest BCR (2.57) was recorded in T2, while the lowest value (2.14) was noted in T4. Therefore, the soil test based   fertilizer dose may be considered as suitable dose for this cropping pattern that ensure higher yield and increase soil fertility. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11226 Bangladesh J. Agril. Res. 37(2): 251-262, June 2012
Physio-Morphological Features of Chilli Accessions Under Moisture Stress Conditions
Authors: Khan MAI , Hoque MA , Farooque AM , Habiba U , Rahim MA
Published: July 2012

An experiment was carried out at the Bangladesh Agricultural University, Mymensingh during October 2005 to March 2007 to study the effect of different soil water levels on the physio-morphological features of ten Chilli (Capsicum annuum L.) accessions viz. C-0277, C-0297, BM-1, C-0100, BM-2, C-0265, C- 0272, C-0275, BM-3, and C-0271. The experiment was set up in pots under glasshouse condition. The water treatments were applied at 4 vegetative growth stages following withholding method (crude method) starting from 25 days after sowing and thereafter at every 7 days interval until final stage. The water treatments applied were W1 = watering once a day; W2 = watering at 4 days interval; W3 = watering at 8 days of interval, and W0 = no watering. Moisture capacity of polybag (analogous to field capacity) was determined by subtracting leached water from the original amount of water applied. In most of the parameters studied, W2 gave the highest value, W1 and W0 gave the lowest, while W3 in between. Only a slight deviation was noticed in case of root volume and root dry weight, where W0 produced the 2nd highest and highest values, respectively. The effects of different water treatments on all the growth parameters studied was significant in case of all accessions and growth stages. The accessions C-0271, C-0277, BM-1, and C-0297 produced higher dry matter. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11229 Bangladesh J. Agril. Res. 37(2): 263-269, June 2012
Effects of Doses and Splits of Fertilizer Application on Harvesting Time, Yield and Quality of Mango CV. Amrapali
Authors: Sarker Babul C , Rahim MA
Published: July 2012

The experiment was carried out at the Germplasm Centre of Bangladesh   Agricultural University, Mymensingh during the fruiting season of 2005-06 to   investigate the effects of fertilizer and its installment of application on   harvesting time, yield and quality of fruits of 8 years old mango plant cv. Amrapali. Four fertilizer doses i.e. T1 : 50% of the fertilizer dose (cowdung 12.5 kg, urea 375 g, TSP200g, MoP125 g, gypsum 125 g and zinc sulphate 7.5 g per plant), T2 : 100% of the fertilizer dose (cowdung 25 kg, urea 750 g, TSP400 g, MoP 250 g, gypsum  250 g and zinc sulphate 15 g per plant), T3 : 150% of the fertilizer dose (cowdung  37.5 kg, urea 1125 g, TSP 600 g, MoP 375 g, gypsum 375 g and zinc sulphate 22.5 g per plant), and T4 : control (no fertilizer) and three splits of application i.e. A1 : One installment (whole fertilizer applied on 15 September), A2 : Two installments (15 September and 15 March) and A3 : Three installments (15 September, 15 March and 15 May) were included as treatments. Plants receiving 150% of the fertilizer dose in three installments caused delayed harvest by 11 days compared  to control than that of the control. Plants treated with 150% of fertilizer dose in combination with three installments produced the highest number of fruits (96/ plant) as well as the highest yield (19.55 kg/plant) as compared to control (23/   plant and 3.48 kg/plant). Applying fertilizer at 150% of the fertilizer dose in  three installments improved the fruit quality with regard to TSS, pH, titratable acidity, vitamin C, moisture content, dry matter content, reducing sugar, non reducing sugar and total sugar content over control. Thus, this treatment may be recommended for fertilizer management in mango cultivation. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11231 Bangladesh J. Agril. Res. 37(2): 279-293, June 2012
Adoption and Diffusion of Power Tillers in Bangladesh
Authors: Quayum MA , Ali Amin Muhammad
Published: July 2012

The present study examines the extent of adoption of power tillers (PT) in  Bangladesh and analyzes the variation in adoption across regions and across farms in selected areas to investigate the justification for wider use of power tillers. Primary and secondary data were used. Primary data were collected from 267 sample farmers from eight villages of four upazilas of four districts using proportionately stratified random sampling technique. Secondary data were collected from different published sources. The average growth rate of power tillers in Bangladesh was 21.0 percent during 1993-2003. Power tillers are unevenly distributed all over the country. The highest and the lowest adoption of power tillers were 44.4 and 3.6 percent in Rajshahi and Barisal divisions respectively. The percentage of area cultivated under power tiller is 69.6. Multiple regression analysis indicates that there is a significant relationship between number of PT and credit availability. The credit availability may be a decisive factor in increasing cropping intensity which requires reduction of turnaround time. Irrigated area, number of small farm holdings and credit availability are found to have significant and positive association with intensity of power tiller use in different regions of Bangladesh. Analysis of Logit Model applied to farm level data indicates that the educational level and income surplus of farmers have significant positive relationship with ownership of power tillers. The coefficients of adult family members and number of draught power owned by the farmers are however found to be significantly negative. The number of power tiller is increasing with the decreasing of draught animal power. Thus credit should be provided to the farmers and owners of all regions of the country  to buy power tillers to adopt evenly to increase crop production profitably. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11234 Bangladesh J. Agril. Res. 37(2): 307-325, June 2012
Identification of Rice Germplasm with Resistance to Bacterial Leaf Blight (Xanthomonas oryzae Pv. Oryzae)
Authors: Sharma B , Pandey MP
Published: July 2012

Not available DOI: http://dx.doi.org/10.3329/bjar.v37i2.11239 Bangladesh J. Agril. Res. 37(2): 349-353, June 2012
Influence of Weather Factors on the Incidence and Distribution of Pumpkin Beetle Infesting Cucurbits
Authors: Khan MMH , Alam MZ , Rahman MM , Miah MIH , Hossain MM
Published: July 2012

Not available DOI: http://dx.doi.org/10.3329/bjar.v37i2.11241 Bangladesh J. Agril. Res. 37(2): 361-367, June 2012
DETERMINATION OF SENSITIVITY AND SPECIFICITY OF IN-HOUSE SANDWICH ELISA FOR THE DETECTION OF INFECTIOUS BURSAL DISEASE VIRUSES
Authors: Saha PK , Ali MH , Rahman MB , Islam MA
Published: July 2012

The study was designed for the development of an In-House sandwich ELISA as a suitable serological method for the rapid detection of infectious bursal disease virus (IBDV). The test was also designed to compare and evaluate its sensitivity and specificity with other traditional methods used for the detection of IBDV from field outbreak cases prevalent among the poultry population of Bangladesh. To develop the In-House sandwich ELISA, hyper-immune serum was raised against live IBDV vaccine in rabbit which was used to coat each of the 96-well flat bottomed polystyrene microtitre plate whereas, hyper-immune sera raised in chickens against IBDV used as secondary antibody. The newly developed In-House sandwich ELISA was standardized by dispensing different dilutions (10-1 up to 10-4) of rabbit serum. Among them, the 10-2 dilution of serum showed most suitable reading for the detection of IBD virus and used to coat the plate to evaluate its sensitivity and specificity. Sensitivity test was done by different dilutions (10-0 to 10-4) of reference IBD virus. The virus dilution, 10-3 was the highest dilution having lowest capacity to bind with coated antibody of the ELISA plate which indicated that IBD viruses was absent in the dilutions of above 10-3. The cut-off value of negative control samples was determined as 0.937 which indicated titer of tested samples >0.937 was positive and <0.937 was negative. Specificity test was performed using different known viruses (IBDV and NDV) using different dilutions (10-1 up to 10-4). Only the IBDV showed positive result which indicated high specificity of newly developed ELISA plate. A total of 26 samples (feces, cloacal swab, spleen and bursa) from control group, experimental and natural IBDV outbreaks were used as field viral antigen for the evaluation of sensitivity and specificity of the newly developed In-House sandwich ELISA. In case of experimental infection, 5 (62.5%) of 8 feces sample but none of cloacal swab were positive for IBDV whereas, all bursa and spleen samples were positive by both InHouse sandwich ELISA and AGIDT. In case of natural outbreak cases, 6 of 6 bursal samples and 4 of 6 spleen samples were positive by In-House sandwich ELISA whereas, AGIDT detected all bursal and 3 spleen samples. No virus was detected from the samples of control group. The result showed 92.85% specificity of the developed sandwich ELISA for detection of IBDV with AGIDT which indicated that the developed ELISA is a sensitive, specific, cost effective and reliable tool for the detection of IBDV antigen from a large number of field samples.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11185 Bangl. J. Vet. Med. (2010). 8 (2) : 97-106
PATHOLOGICALSTUDY ON COLIBACILLOSIS IN CHICKENS AND DETECTION OF ESCHERICHIA COLI BY PCR
Authors: Tonu NS , Sufian MA , Sarker S , Kamal MM , Rahman MH , Hossain MM
Published: July 2012

The aim of the present study was to detect the pathogenic Escherichia coli (E. coli) through pathological study of the colibacillosis affected birds. These isolated E. coli were further confirmed by PCR using specific primer. For this purpose, a total of 20 swabs (10 from lung and 10 from intestine of 10 dead birds) were collected in sterile nutrient broth. The histopathological samples were collected in 10% buffered neutral formalin. The used methods were histopathology, isolation and identification of E. coli by conventional methods and as well as by PCR method. A total of 10 isolates of E. coli from 20 swabs of lung and intestine was characterized by conventional routine methods of bacteriology. Gross pathological lesions of all lungs in the present investigation were congested and consolidated. Duodenum showed congestion and hemorrhages with excess mucus in the luminal surface of it. Microscopically, all the lungs showed severe congestion, infiltration of heterophils, macrophages and lymphocytes in the wall of bronchus as well as in the peribronchial alveoli. E. coli infected all the duodenum showed severe infiltration of leukocytes mainly heterophils, lymphocytes and macrophages in the submucosa of the duodenal wall. In this study, DNA of 8 isolates out of 10 isolated E. coli organisms was amplified by PCR using ECO-f and ECO-r primer targeting 16S ribosomal DNA and found 585 bp amplicon which is specific for E. coli with enteroinvasive type confirmed by histopathological lesions in duodenum. Further investigation should be focused on serotyping and detection of genes of E. coli which are responsible for pathogenicity of the organism.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11205Bangl. J. Vet. Med. (2011). 9(1): 17-25
EXPERIMENTAL PRODUCTION OF NECROTIC ENTERITIS IN BROILER CHICKENS
Authors: Asaduzzaman M , Miah MS , Siddika A , Popy N , Hossain MM
Published: July 2012

The present study was conducted to isolate and identify the Clostridium perfringens, the etiology of necrotic enteritis (NE) from broiler chickens and experimental production of NE with this isolate. A total of 50 samples were collected from jejunum of necropsied birds for isolation and identification of Cl. perfringens. Out of 50 samples, only 4 isolates of Cl. perfringens were isolated and identified (prevalence 8%). In experimental NE, the birds of group A (orally administered with only 0.1 ml (1x103 sporulated Eimeria acervulina oocysts/bird) showed dullness, ruffled feather, vent feather soiled with bloody faeces after 1 week of coccidial challenge. The birds of group B (orally administered 1x103 sporulated Eimeria acervulina oocysts/bird and 1 ml of 2 days old broth culture of Cl. perfringens) showed severe depression, ruffled feathers, bloody faeces with fibrinous cast with 80% prevalence rate and 30% mortality in experimental NE. The birds of group C (orally inoculated with 1 ml of 2 days old broth culture of Cl. perfringens) showed no striking clinical, gross and histopathological lesions.  Postmortem changes in small intestine (duodenum) were congestion and haemorrhages specially for birds of group A. The most severe gross lesions comprised of ascites, enlarged liver and heart, intestinal distension, profuse haemorrhage, fibrinous cast, fragile intestinal wall and gas bubble formation in the small intestine (duodenum, jejunum and ileum) of birds of group B. Histologically, birds of group B showed hemorrhage and congestion in liver, heart and intestine, desquamation of intestinal epithelium and intense leukocytic infiltration in intestine, liver and heart. The findings obtained from this study showed that simultaneous coccidial infection enhanced the pathological lesions of NE. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11207Bangl. J. Vet. Med. (2011). 9(1): 3341
HISTOMORPHOLOGICAL STUDY OF THE MAJOR LYMPHOID TISSUES IN INDIGENOUS DUCKLINGS OF BANGLADESH
Authors: Sultana N , Khan MZI , Wares MA , Masum MA
Published: July 2012

A histomorphological study was performed in the major lymphoid tissues (thymus, bursa of Fabricus and spleen) of the six 21-day-old indigenous ducklings of Bangladesh by H & E staining method during the period from March to May 2011. In the present study, it was observed that the thymus was enclosed by a thin connective tissue capsule. Numerous fine septa of connective tissue originated from the capsule and divided the organ into incompletely separated lobules. Each lobule organized into a peripheral cortex and a central medulla. The bursa of Fabricus was consisted of mucosal folds (plicae). Numerous follicles filled the lamina propria of each fold and each bursal follicle was composed a peripheral cortex and a central medulla. A layer of undifferentiated epithelial cells occupied the periphery of the medulla, which was separated from the cortex by a capillary layer. The darkly stained cortex was composed of many closely packed small lymphocytes. The paler medulla contained fewer cells of various sizes. The spleen was surrounded by a thick splenic capsule and there were a small number of trabeculae. The white pulp was composed of network of reticular cells and reticular fibers within various size lymphocytes and plasma cells were diffusely distributed. The red pulp of the spleen was formed from venous sinuses and anastomosing cord of reticular cells, macrophages, lymphocytes and blood cells. The length and breadth of the thymic lobules, bursal follicles and white pulp of the spleen were 226.68 and 165.78cm, 204.45 and 138.23cm, and 129.05 and 103.43cm respectively. The result of the present work revealed that the immunocompetent cells were arranged scatteredly or densely as an unorganized lymphatic nodules in the lymphoid tissues. The length and breadth of the thymic lobules were higher followed by bursal follicle and splenic white pulps were varied within the lymphoid tissues and even one another in indigenous ducklings. The results of the present study indicate that the architecture and distribution of lymphocytes and lymphoid follicles of ducklings is very close to the chicken and this study might be helpful to understand the changes in the frequency of the population of immunocompetent cells in drug induced, vitamin and mineral supplemented or hormone treated duck in future.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11212 Bangl. J. Vet. Med. (2011). 9(1): 53-58
EFFECTS OF CHRONIC ARSENIC TOXICITY ON THE HAEMATOLOGY AND HISTOARCHITECTURE OF FEMALE REPRODUCTIVE SYSTEM OF BLACK BENGAL GOAT
Authors: Islam MT , Parvin S , Pervin M , Bari ASM , Khan MAHNA
Published: July 2012

This study was aimed to observe the effects of chronic arsenic toxicity on the haematology and histoarchitecture of female reproductive system of black Bengal goat. Adult She goats (N=4) were orally administered with sodium arsenite (4 mg/kg body weight) for first seven weeks followed by 5 mg/kg body weight for next eight weeks. Blood was collected from the treated goat at day0, 20, 40, 60, 80 and 100. There was significant difference of TEC, Hb concentration and TLC in goats which are chronically exposed with arsenic. Lymphocyte was decreased with the treatment of sodium arsenite but neutrophil, eosinophil and monocyte were increased. At the end of study the goats were euthanasized. The ovary, uterine tube, uterus, cervix and vagina were collected for gross and histopathological examinations. In histopathological examination the ovarian follicles appeared degenerated and there was much stromal thickening around the follicle. Thickened myometrial layer and shortened mucosal folds in the uterine tube were observed. There was reduction in the number and size of endometrial glands. The mucosal cell lines appeared degenerated. Thin vaginal mucosa and proliferation of connective tissue; shortened cervical crypts and thickened glandular epithelium of cervix were also observed. It may be concluded that chronic arsenic exposure might have adverse effects on the female reproductive system.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11214 Bangl. J. Vet. Med. (2011). 9(1): 59-66
PREVALENCE OF SUBCLINICAL MASTITIS IN DAIRY COWS IN SELECTED AREAS OF BANGLADESH
Authors: Islam MA , Islam MZ , Islam MA , Rahman MS , Islam MT
Published: July 2012

This research was carried out to determine the prevalence of subclinical mastitis in lactating Dairy Cow of Bangladesh Agricultural University dairy farm (BAUDF) and rural areas of Tangail sadar upazila of Bangladesh during the period of July 2009 to April 2010. A total of 200 milk samples (40 from BAUDF and 160 from Tangail sadar upazila) were collected for this study which were subjected to physical examination and subsequently screened for subclinical mastitis using three indirect tests viz. White Side Test (WST), California Mastitis Test (CMT), and Surf Field Mastitis Test (SFMT). Overall prevalence of subclinical mastitis (SCM) in lactating dairy cows found in this study was 29%. Cows were infected with SCM 29.5%, 27.5% and 25.5% detection by CMT, WST and SFMT respectively. Higher prevalence of SCM was detected in milch crossbred cows (36.36%) in comparison to local bred cows (24.61%) maintained under extensive management system in Rural area of Tangail sadar upazila. The prevalence of SCM was recorded in 31.58%, 30.76% and 68.75% in cows of local area of Tangail sadar upazila, and 25.0%, 40.0% and 71.42% in cows of BAU,DF during the early, mid and late stages of lactation respectively. The highest prevalence of SCM was recorded during the early lactation stage in both the local breed cows (30.0%) and cows of BAUDF (45.83%) in comparison to their respective mid and late stages of lactation. The prevalence of SCM was highest in lactating cows having third lactation and high yielding (cows produced >10 liter milk per day) both in local breed and crossbred cows.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11216 Bangl. J. Vet. Med. (2011). 9(1): 73-78
Preclinical Cancer Chemoprevention Studies Using Animal Model of Inflammation-Associated Colorectal Carcinogenesis
Authors: Tanaka Takuji
Published: July 2012

Inflammation is involved in all stages of carcinogenesis. Inflammatory bowel disease, such as ulcerative colitis and Crohn’s disease is a longstanding inflammatory disease of intestine with increased risk for colorectal cancer (CRC). Several molecular events involved in chronic inflammatory process are reported to contribute to multi-step carcinogenesis of CRC in the inflamed colon. They include over-production of free radicals, reactive oxygen and nitrogen species, up-regulation of inflammatory enzymes in arachidonic acid biosynthesis pathway, up-regulation of certain cytokines, and intestinal immune system dysfunction. In this article, firstly I briefly introduce our experimental animal models where colorectal neoplasms rapidly develop in the inflamed colorectum. Secondary, data on preclinical cancer chemoprevention studies of inflammation-associated colon carcinogenesis by morin, bezafibrate, and valproic acid, using this novel inflammation-related colorectal carcinogenesis model is described.
1,5-Diaminopentane As A Structure-Directing Agent for Zincophosphate Networks: Zn3(PO4)2(C5H14N2)2·3H2O and C5H16N2·Zn3(PO4)2(HPO4)·H2O
Authors: Harrison William T.A. , Currie William R.
Published: July 2012

The crystal structures of two zincophosphate networks prepared in the presence of 1,5-diaminopentane (dap) are described. In Zn3(PO4)2(C5H14N2)2·3H2O (1) the dap forms Zn–N coordinate bonds to generate an unusual three-dimensional “hybrid” framework constructed from ZnO3N, ZnO2N2 and PO4 tetrahedra with three different types of elongated channels occupied by water molecules. In C5H16N2·Zn3(PO4)2(HPO4)·H2O; (2) the doubly-protonated H2dap acts in a more typical way to template double layers of vertex-sharing ZnO4, PO4 and HPO4 tetrahedra incorporating 10-rings and interacts with the inorganic component via N–H O hydrogen bonds. Crystal data: 1 (C10H34N4O11P2Zn3), Mr = 644.46, monoclinic, C2 (No. 4), Z = 4, a = 25.302 (7) Å, b = 4.9327 (13) Å, c = 19.808 (6) Å, b = 107.377 (8)°, V = 2359.4 (12) Å3, R(F) = 0.054, wR(F2) = 0.139. 2 (C5H19N2O13P3Zn3), Mr = 604.24, monoclinic, P21/c (No. 14), Z = 4, a = 11.3275 (15) Å, b = 8.3235 (11) Å, c = 18.588 (2) Å, b = 96.979 (3)°, V = 1739.6 (4) Å3, R(F) = 0.056, wR(F2) = 0.119.
Microwave-Assisted Method for Simultaneous Extraction and Hydrolysis for Determination of Flavonol Glycosides in Ginkgo Foliage Using Brönsted Acidic Ionic-Liquid [HO3S(CH2)4mim]HSO4 Aqueous Solutions
Authors: Yao Huanhuan , Du Xinxuan , Yang Lei , Wang Wenjie , Yang Fengjian , Zhao Chunjian , Meng Xiangdong , Zhang Lin , Zu Yuangang
Published: July 2012

The Brönsted acidic ionic-liquid [HO3S(CH2)4mim] HSO4, a novel dual catalyst–solvent, has been successfully applied in simultaneous microwave-assisted extraction and hydrolysis for the determination of flavonol glycosides in Ginkgo foliage. The parameters, namely the [HO3S(CH2)4mim]HSO4 concentration, microwave-irradiation power, microwave-irradiation time, and solid–liquid ratio, were optimized. The optimum conditions were: an amount of 1.5 M [HO3S(CH2)4mim]HSO4, a microwave-irradiation power of 120 W, an irradiation time of 15 min, and a solid–liquid ratio of 1:30 g/mL. Compared with traditional methods the proposed approach demonstrates higher efficiency in a shorter operating time, and is an efficient, rapid, and simple sample preparation method.
Isolation and Characterization of Cross-Amplification Microsatellite Panels for Species of Procapra (Bovidae; Antilopinae)
Authors: Chen Jing , Li Chunlin , Yang Ji , Luo Zhenhua , Tang Songhua , Li Feng , Li Chunwang , Liu Bingwan , Jiang Zhigang
Published: July 2012

The three Procapra species, Tibetan gazelle (P. picticaudata), Mongolian gazelle (P. gutturosa) and Przewalski’s gazelle (P. przewalskii) are endemic to Asia. Several intraspecific genetic issues have been studied with species-specific microsatellite loci in these Asian gazelles. However, cross-species microsatellite panels are absent, which inhibits comparative conservation and evolutionary studies of the Procapra. In this study, we isolated 20 cross-species microsatellite loci for Procapra from both related species and the genomic library of P. przewalskii. Fifty-three samples of the three gazelles were used to characterize the markers. Allele numbers ranged from three to 20, with a mean of 7.93 per locus. Observed heterozygosity (HO) averaged 0.680 and expected heterozygosity (HE) 0.767. The mean polymorphic information content (PIC) was 0.757 for P. picticaudata, 0.803 for P. gutturosa and 0.590 for P. przewalskii. Nine loci were significantly deviated from Hardy-Weinberg (H-W) equilibrium in the three species. Significant linkage disequilibrium was detected in four pairs of loci in P. przewalskii, five pairs in P. gutturosa and 51 pairs in P. picticaudata. Considering the abundance of published loci and their high success rates of cross-amplification, testing and utilization of loci from related species is efficient for wild species of Bovidae. The cross-species microsatellite loci we developed will facilitate further interspecies genetic studies in Procapra.
Development of Metabolic Indicators of Burn Injury: Very Low Density Lipoprotein (VLDL) and Acetoacetate Are Highly Correlated to Severity of Burn Injury in Rats
Authors: Izamis Maria-Louisa , Uygun Korkut , Sharma Nripen S. , Uygun Basak , Yarmush Martin L. , Berthiaume Francois
Published: July 2012

Hypermetabolism is a significant sequela to severe trauma such as burns, as well as critical illnesses such as cancer. It persists in parallel to, or beyond, the original pathology for many months as an often-fatal comorbidity. Currently, diagnosis is based solely on clinical observations of increased energy expenditure, severe muscle wasting and progressive organ dysfunction. In order to identify the minimum number of necessary variables, and to develop a rat model of burn injury-induced hypermetabolism, we utilized data mining approaches to identify the metabolic variables that strongly correlate to the severity of injury. A clustering-based algorithm was introduced into a regression model of the extent of burn injury. As a result, a neural network model which employs VLDL and acetoacetate levels was demonstrated to predict the extent of burn injury with 88% accuracy in the rat model. The physiological importance of the identified variables in the context of hypermetabolism, and necessary steps in extension of this preliminary model to a clinically utilizable index of severity of burn injury are outlined.
Influence of ShuJinHuoXue Tablets on Ischemia Reperfusion Injury of Animals’ Skeletal Muscle
Authors: Tong Zhihong , Yu Fang , Liu Zhonghua , Liang Haidong
Published: July 2012

Ischemia-reperfusion (IR) can lead to serious tissue oxidative injury in animals. ShuJinHuoXue tablet (SJHXT) is a Chinese Traditional Medicine which can relax the muscles and stimulate the blood circulation and has been used as a clinical medicine. In the present study, we investigated the effects of SJHXT pretreatment on oxidative injury using an animal model of acute limb IR. Results showed that SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced serum endothelin-1 (ET-1), thromboxane B2 (TXB2) levels and thromboxane B2/6-keto- prostaglandin F1α (TXB2/6-Keto-PGF1α), wet weight/dried weight (W/D) ratio, myeloperoxidase (MPO), creatine kinase (CK), lactate dehydrogenase (LDH) activities, and increased serum nitric oxide (NO), 6-Keto-PGF1α levels and NO/ET-1 ratio in the IR+SJHXT groups. In addition, the SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced skeletal muscle Ca2+, malondialdehyde (MDA) levels, increased Na+-K+-ATPase, Ca2+-Mg2+-ATPase, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. Our results suggest that SJHXT pre-treatment may improve skeletal muscle blood vessel microcirculation, decrease skeletal muscle oxidative injury and enhance antioxidant enzymes activities in IR animals.