bluuebunny/crossref_metadata_2025 huggingface dataset:
Major Vault Protein/Lung Resistance-Related Protein: A Novel Biomarker for Inflammation and Acute Infections
Authors: Routsias John G. , Marinou Dionysia , Mavrouli Maria , Tsakris Athanasios , Pitiriga Vassiliki C.
Published: August 2024

Introduction: Vault particles are large cytoplasmic ribonucleoprotein particles that participate in inflammation. The aim of this study was to assess the diagnostic and prognostic value of major vault protein (MVP) in patients with inflammation, in order to determine whether MVP could be used as a biomarker for infection or inflammation. We also aimed to compare the diagnostic impact of MVP compared to other conventional measurements, such as CRP or white blood cell (WBC) counts. Methods: CRP and MVP levels were measured in 111 sera samples from 85 patients with inflammation admitted to a tertiary-care hospital and 26 healthy individuals during an 18-month period (2019–2020), using nephelometry and a custom MVP sandwich ELISA assay, respectively. In addition, WBC counts were measured using a commercial assay. Results: MVP levels were found to be elevated in patients with inflammation compared to healthy individuals (p < 0.0001). Moreover, MVP levels were higher in patients with inflammation due to an infectious etiology compared to those with non-infectious etiology (p = 0.0006). MVP levels significantly decreased during the first four days of infection in response to antibiotic treatment, while CRP levels showed a less-sensitive decline. An ROC curve analysis demonstrated that MVP and CRP have similarly high diagnostic accuracy, with AUCs of 0.955 and 0.995, respectively, followed by WBCs with an AUC of 0.805. Conclusions: The ROC curves demonstrated that MVP has the potential to serve as a diagnostic biomarker for inflammation and infection. Additionally, MVP levels may reflect the efficacy of antibiotic treatment.
AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans
Authors: Linghu Shao-Xiang , Zhang Yu , Zuo Jia-Fang , Mo Ming-He , Li Guo-Hong
Published: August 2024

Nematode-trapping (NT) fungi are a major resource for controlling parasitic nematodes. Arthrobotrys flagrans, as a typical NT fungus, can capture nematodes by producing three-dimensional nets. The APSES transcription factor Swi6 plays a vital role in fungal growth and the pathogenicity of pathogens. In this study, we characterized AfSwi6 via gene disruption using the homologous recombinant method and transcriptome sequencing. Knockout of the AfSwi6 gene caused defects in mycelial growth, trap formation and pathogenicity, chlamydospore production, and stress response. Moreover, the transcriptome data indicated that AfSwi6 was related to DNA repair, stress response, and plasma membrane fusion. The result showed that AfSwi6 has a significant effect on trap development and chlamydospore production in A. flagrans.
Evidence for the Presence of Borrelia burgdorferi Biofilm in Infected Mouse Heart Tissues
Authors: Thippani Sahaja , Patel Niraj Jatin , Jathan Jasmine , Filush Kate , Socarras Kayla M. , DiLorenzo Jessica , Balasubramanian Kunthavai , Gupta Khusali , Ortiz Aleman Geneve , Pandya Jay M. , Kavitapu Venkata V. , Zeng Daina , Miller Jennifer C. , Sapi Eva
Published: August 2024

Borrelia burgdorferi, the bacterium responsible for Lyme disease, has been shown to form antimicrobial-tolerant biofilms, which protect it from unfavorable conditions. Bacterial biofilms are known to significantly contribute to severe inflammation, such as carditis, a common manifestation of Lyme disease. However, the role of B. burgdorferi biofilms in the development of Lyme carditis has not been thoroughly investigated due to the absence of an appropriate model system. In this study, we examined heart tissues from mice infected with B. burgdorferi for the presence of biofilms and inflammatory markers using immunohistochemistry (IHC), combined fluorescence in situ hybridization FISH/IHC, 3D microscopy, and atomic force microscopy techniques. Our results reveal that B. burgdorferi spirochetes form aggregates with a known biofilm marker (alginate) in mouse heart tissues. Furthermore, these biofilms induce inflammation, as indicated by elevated levels of murine C-reactive protein near the biofilms. This research provides evidence that B. burgdorferi can form biofilms in mouse heart tissue and trigger inflammatory processes, suggesting that the mouse model is a valuable tool for future studies on B. burgdorferi biofilms.
Application of Nanofibrous Clay Minerals in Water-Based Drilling Fluids: Principles, Methods, and Challenges
Authors: Zhuang Guanzheng , Zhang Jiajun , Chen Jinrong , Liu Qian , Fan Wenxiao , Li Qiang
Published: August 2024

Nanofibrous clay minerals, specifically palygorskite (Pal) and sepiolite (Sep), have been becoming a new generation of rheological additives for drilling fluid systems due to their unique nanostructure, high performance, environmentally benign nature, and cost-effectiveness. These nanoclay minerals exhibit excellent colloidal and rheological properties in aqueous systems, even in saline and high-temperature environments. Although Pal and Sep have been employed as auxiliary rheological additives in a few cases to enhance the salt resistance of conventional water-based drilling fluids (WBDFs), these two clay minerals have not yet been used on a large scale due to a lack of understanding of their structures and properties, as well as the control of their performance. This paper presents a comprehensive review of the clay mineralogy, colloidal chemistry, rheological behaviors, and filtration properties of nanofibrous clay minerals in WBDFs, with critical comments. It also discusses the challenges and prospects for further research. This review provides new insights into fundamental and applied studies of nanofibrous clay minerals and helps promote the large-scale application of nanofibrous clay products in drilling fluids.
An Approach to Accurately Identifying Binders in Historic Mortars by the Combination of Microscopic and Microanalytical Techniques
Authors: Almeida Luís , Santos Silva António , Veiga Rosário , Mirão José
Published: August 2024

Mortars are among the most important materials in building construction. They are generally obtained by mixing aggregates with an inorganic binder. The identification of mortar constituents, particularly the binder type in historic buildings, is one of the essential aspects of building conservation, considering that the new conservation materials must be chemically, mechanically, and physically compatible with the old masonries. Among other techniques used to characterise binders, those related to optical and electronic microscopy are particularly important. Microscopy and combined techniques may be the key to this identification since the classic mineralogical and chemical-based identification approaches are not conclusive enough in investigating the types of hydraulic binders in mortars. This work presents an analysis procedure to identify mortar binders by combining EDS microanalysis and petrography. Mortar samples of known composition were used as a reference for analyzing mortars from historic buildings. The proposed methodology made it possible to identify the type of binder or a mixture of binders based on the identification of the binder features by petrography together with analysis of the chemical composition of the paste by X-ray microanalysis under a scanning electron microscope.
Cu–Polymetallic Deposit Exploration under Thick Cover in Gucheng–Yaxi Area Using Audio-Magnetotelluric and Spread-Spectrum-Induced Polarization
Authors: Ullah Farid , Zhou Xin , Chen Rujun , Yang Lunkai , Yao Hongchun , Hu Hao , Chen Siwen , Wang Quangong
Published: August 2024

Successful geophysical exploration projects in the Gucheng–Yaxi area located in Gaochun District, Jiangsu Province, China, have been limited partly due to the complex geological conditions of the area and high artificial noise in data acquired using electrical and electromagnetic methods. In this study, we deployed the new anti-interference spread-spectrum-induced polarization method (SSIP) and the audio-magnetotelluric (AMT) method to detect a copper–polymetallic deposit in the area. Two-dimensional inversion results in the Gucheng–Yaxi area revealed a high chargeability anomalous zone on the SSIP profile that coincided with a zone of moderate resistivity located between two resistor bodies on the AMT profile. A follow-up 1200 m drill hole was established at this high-chargeability, moderate-resistivity zone which encountered polymetallic (copper, lead, zinc, gold, and silver) mineralization at a depth of ≥400 m. Drill hole data analysis showed that mineralization occurred interspaced in the marble rock mass at varying depths. Furthermore, several low-resistivity, weak-chargeability sections were revealed and attributed to Cretaceous sediments and faults. These faults are thought to have played a critical role in the polymetallic mineralization genesis. In summary, this study demonstrated the successful of application of SSIP and AMT in detecting a metallic deposit in an area with high artificial noise. Hence, the geophysical prospection potential of the Gucheng–Yaxi area is great.
Elemental Geochemistry and Pb Isotopic Compositions of the Thick No. 7 Coal Seam in the Datun Mining Area, China
Authors: Meng Na , Xiao Qianlong , Li Wu
Published: August 2024

Thick coal seams recorded abundant petrological, geochemical, and mineralogical information regarding their formation, which in turn can reflect the characteristics of the coal-forming environments, provenance attributes, paleoclimate, and so on. In order to explore the geochemical and lead isotope characteristics of thick coal seams, the No. 7 coal seam in the Datun mining area, Jiangsu Province of China, was selected as the research object. In this work, 29 samples (including coal, roof, and floor rock samples) were collected from three coal mines in the Datun mining area. Through an analysis of the mineral composition and element geochemical characteristics in the coal samples, the enrichment degree of trace elements and modes of rare earth elements were determined. The genetic mechanism of abnormal enrichment of enriched elements is discussed, especially the modes of occurrence and isotope characteristics of Pb. The results showed the following: (1) The main minerals in the coal samples include quartz, potassium feldspar, plagioclase, calcite, dolomite, pyrite, gypsum, and clay minerals, with clay minerals, calcite, quartz, and dolomite being the most common. (2) The major element oxides in coal mainly include SiO2, Al2O3, Fe2O3, MgO, CaO, Na2O, K2O, TiO2, P2O5, and FeO. In the vertical direction, the variation of SiO2, Al2O3, Fe2O3, MgO, K2O, and FeO in coal samples from the three coal mines is consistent. The average value of Al2O3/TiO2 in the samples of Kongzhuang, Longdong, and Yaoqiao coal mines is 28.09–50.52, which basically locates the samples in the felsic source area, such that the sediment source is considered to be felsic source rock. (3) Elements U, La, Pb, and other elements are more enriched in Kongzhuang coal mine samples; elements Th, U, La, Pb, and other elements are more enriched in the Longdong coal mine samples; and elements Th, U, La, Pb, and other elements are more enriched in the Yaoqiao coal mine samples. Furthermore, W is enriched in Yaoqiao mine samples and is highly enriched in Longdong mine samples. The mining area is generally rich in the elements U, La, and Pb. The distribution curves of rare earth elements in the three mines are inclined to the right, with negative Eu anomalies. The enrichment is of the light rare earth enrichment type. (4) Pb isotope data show that the samples from the three mines are mainly distributed in the orogenic belt and the subduction zone lead source areas, where the upper crust and the mantle are mixed, with individual sample points distributed in the mantle and upper crust lead source areas.
The Characteristics and Enrichment Process of Dabu Ion-Adsorption Heavy Rare-Earth Element (HREE) Deposits in Jiangxi Province, South China
Authors: Xie Mingjun , Zhou Jian , Du Xuemiao , Wang Xueqiu , Zhang Bimin , Wu Hui , Hu Qinghai , Wang Wei , Tian Mi , Chen Binfeng , Mo Huohua , Wang Lijun
Published: August 2024

Ion-adsorption rare-earth deposits supply over 90% of the global market’s heavy rare-earth elements (HREEs). The genesis of these deposits, particularly HREE deposits, has garnered significant attention. To elucidate the metallogenic mechanisms of HREE deposits, a comprehensive study of the weathering profile of granite was conducted in Jiangxi Province, South China. This study focuses on the following two aspects: the petrogeochemistry of HREE-rich granite and the enrichment and fractionation of rare-earth elements (REEs) during the weathering process. The results suggest that the Dabu granites are a typical peraluminous, high-K, calc-alkaline granite series with high silica content (SiO2: 74.5%–76.4%), relatively low phosphorus content (P2O5: <0.05%), and high HREE content (ΣLREE/ΣHREE: 0.16–0.66). Weathering advances the decomposition of minerals and the release of elements. REEs are mainly fixed in the regolith by scavengers, mainly clays, Fe–Mn oxides, and carbonates, and ΣREE can reach 799 ppm in the B horizon. However, HREEs tend to migrate further and preferentially combine with Fe–Mn oxides and carbonates as compared to LREEs, leading to a significant fractionation of REEs in the regolith (ΣLREE/ΣHREE = 0.2–1.1). Additionally, the differential weathering of REE-bearing minerals and the precipitation of secondary REE-bearing minerals are also vital for REE fractionation.
Updating Geological Information about the Metallogenesis of the Iberian Pyrite Belt
Authors: Sáez Reinaldo , González Felipe , Donaire Teodosio , Toscano Manuel , Yesares Lola , de Almodóvar Gabriel Ruiz , Moreno Carmen
Published: August 2024

The Iberian Pyrite Belt (IPB) represents one of the largest districts of volcanogenic massive sulfide (VMS) deposits in the world, and is a critical source of base metals (Cu, Pb, and Zn) for Europe. Confirmed resources exceed 1700 Mt of massive sulfides with grades of around 1.2% Cu, 1% Pb, and 3% Zn as well as more than 300 Mt of stockwork-type copper mineralization. Significant resources of Sn, precious metals (Au and Ag), and critical metals (Co, Bi, Sb, In, and Se) have also been evaluated. The genesis of these deposits is related to a complex geological evolution during the late Devonian and Mississippian periods. The geological record of such evolution is represented by three main lithological units: Phyllite–Quartzite Group, the volcano–sedimentary Complex (VSC), and the so-called Culm Group. The sulfide deposits are located in the VSC, associated with felsic volcanic rocks or sedimentary rocks such as black shales. The massive sulfide deposits occur as tabular bodies and replacement masses associated with both volcanic and sedimentary rocks. Their mineralogical composition is relatively simple, dominated by pyrite, chalcopyrite, sphalerite, and galena. Their origin is related to three evolutionary stages at increasing temperatures, and a subsequent stage associated with the Variscan deformation. The present paper summarizes the latest developments in the IPB and revises research areas requiring further investigation.
Aggression to Biomembranes by Hydrophobic Tail Chains under the Stimulus of Reductant
Authors: Wang Sijia , Xu Huifang , Li Yuanyuan , Zhang Lingyi , Xu Shouhong
Published: August 2024

Stimulus-responsive materials hold significant promise for antitumor applications due to their variable structures and physical properties. In this paper, a series of peptides with a responsive viologen derivative, Pep-CnV (n = 1, 2, 3) were designed and synthesized. The process and mechanism of the interaction were studied and discussed. An ultraviolet–visible (UV) spectrophotometer and fluorescence spectrophotometer were used to study their redox responsiveness. Additionally, their secondary structures were measured by Circular Dichroism (CD) in the presence or absence of the reductant, Na2SO3. DPPC and DPPG liposomes were prepared to mimic normal and tumor cell membranes. The interaction between Pep-CnV and biomembranes was investigated by the measurements of surface tension and cargo leakage. Results proved Pep-CnV was more likely to interact with the DPPG liposome and destroy its biomembrane under the stimulus of the reductant. And the destruction increased with the length of the hydrophobic tail chain. Pep-CnV showed its potential as an intelligent antitumor agent.
Study on Soot and NOx Formation Characteristics in Ammonia/Ethylene Laminar Co-Flow Diffusion Flame
Authors: Li Shuanglong , Liu Qianqian , Zhang Feng , Sun Jingyun , Wang Yang , Gu Mingyan
Published: August 2024

The formation of soot and NOx in ammonia/ethylene flames with varying ammonia ratios was investigated through experimental and numerical analysis. The spatial distribution of the soot volume fraction and NOx concentrations along the flame central line were measured, and the mechanism of soot and NOx formation during ammonia/ethylene co-combustion was analyzed using CHEMKIN 17.0. The experimental results indicated that the soot volume fraction decreases with an increase in ammonia ratio, with the soot peak concentration occurring in the upper region of the flame. The distribution of NOx is complex. In the initial part of the flame, a higher concentration of NOx is generated, and the lower the ammonia ratio, the higher the concentration of NOx. As the combustion process progresses, the concentration of NOx initially decreases and then subsequently increases rapidly, with higher ammonia ratios leading to higher concentrations of NOx. The addition of ammonia results in a decrease in CH3, C2H2, and C3H3, and an increase in CN concentration. This leads to a transformation of carbon atoms within the combustion system, reducing the available carbon for soot formation and suppressing its generation. A higher ammonia ratio increases the likelihood that NH3 will be oxidized to N2, as well as increasing the probability that any generated NO will undergo reduction to N2 through the action of the free radicals NH2 and NH.
Measuring the Equilibrium Spreading Pressure—A Tale of Three Amphiphiles
Authors: Peychev Boyan , Arabadzhieva Dimitrinka , Minkov Ivan L. , Dimitrova Iglika M. , Mileva Elena , Smoukov Stoyan K. , Slavchov Radomir I.
Published: August 2024

A surfactant’s equilibrium spreading pressure (ESP) is the maximum decrease in surface tension achievable at equilibrium below the Krafft point. Difficulties in measuring the ESP have been noted previously but no well-established experimental protocols to overcome them exist. We present a case study of three solid amphiphiles with different propensities to spread on the air–water interface. Starting with the partially water soluble n-dodecanol (C12H25OH), which spreads instantaneously. The strong Marangoni flows associated with the spreading result in the dislocating of the Wilhelmy plate or crystals attaching to it. A temporary mechanical barrier in front of the spreading crystals mitigates the flows disturbing the plate. Presaturating the subphase with the amphiphile prevents the establishment of dynamic steady states, reduces the standard error by a factor of three and causes faster equilibration. The perfluoroalkylated analog of dodecanol (11:1 fluorotelomer alcohol, C11F23CH2OH) is slow spreading. With surfactant crystals on the interface, the surface pressure reaches a pre-equilibrium plateau within an hour, followed by equilibration on day-long timescales. We show that it is better to estimate the ESP by averaging the values of multiple pre-equilibrium plateaus rather than waiting for equilibrium to be established. Finally, the nonspreading amphiphile DPPC exhibits a large barrier for the mass transfer from the DPPC crystal to the aqueous surface. This was overcome by introducing a volatile, water-immiscible solvent deposited on the surface next to the crystals to facilitate the spreading process and leave behind a monolayer.
Copper(II) Methacrylate Complexes with Imidazole Derivatives—Structural, Spectral and Antitumor Features
Authors: Teodoru Dragoș Vlad , Olar Rodica , Maxim Cătălin , Bacalum Mihaela , Răileanu Mina , Iorgulescu Emilia-Elena , Vasile Scăețeanu Gina , Badea Mihaela
Published: August 2024

A series of five novel copper(II) complexes with imidazole derivatives having general core Cu(R-Im)2(Macr)2 (Macr = methacrylate anion; R-Im = 2-methylimidazole/2-MeIm, 4-methylimidazole/4-MeIm, 2-ethylimidazole/2-EtIm, 2-isopropylimidazole/2-iPrIm) has been synthesized and characterized by elemental analysis, Fourier Transform Infrared spectroscopy (FTIR), electronic reflectance spectroscopy, cyclic voltammetry, thermal analysis and single crystal X-ray diffraction. All complexes crystalize in a monoclinic crystal system and form a complex supramolecular network developed through hydrogen bonds. The stereochemistry of the copper ion is distorted octahedral except for the compound with 4-methylimidazole for which the geometry is square-pyramidal. The imidazole derivatives act as unidentate while methacrylate ions are chelated except for compound with 4-methylimidazole where is unidentate. All ligands and complexes inhibited B16 murine melanoma cells in a micromolar range, but the complex with 2-isopropylimidazole was more active. Furthermore, all species do not affect the healthy BJ cells in the concentration range used for assays.
Synthesis and Biological Evaluation of Novel Piperidine-3-Carboxamide Derivatives as Anti-Osteoporosis Agents Targeting Cathepsin K
Authors: Wang Yali , Guan Ting , Xiong Hegen , Hu Wenxin , Zhu Xianjian , Ma Yuanyuan , Zhang Zhiqing
Published: August 2024

A series of novel piperidamide-3-carboxamide derivatives were synthesized and evaluated for their inhibitory activities against cathepsin K. Among these derivatives, compound H-9 exhibited the most potent inhibition, with an IC50 value of 0.08 µM. Molecular docking studies revealed that H-9 formed several hydrogen bonds and hydrophobic interactions with key active-site residues of cathepsin K. In vitro, H-9 demonstrated anti-bone resorption effects that were comparable to those of MIV-711, a cathepsin K inhibitor currently in phase 2a clinical trials for the treatment of bone metabolic disease. Western blot analysis confirmed that H-9 effectively downregulated cathepsin K expression in RANKL-reduced RAW264.7 cells. Moreover, in vivo experiments showed that H-9 increased the bone mineral density of OVX-induced osteoporosis mice. These results suggest that H-9 is a potent anti-bone resorption agent targeting cathepsin K and warrants further investigation for its potential anti-osteoporosis values.
A Convenient Synthesis of Short α-/β-Mixed Peptides as Potential α-Amylase Inhibitors
Authors: Ahmed Naeem , Razzaq Fakhira , Arfan Muhammad , Gatasheh Mansour K. , Nasir Hammad , Ali Joham Sarfraz , Hafeez Hamna
Published: August 2024

Over the last decades, the increased incidence of metabolic disorders, such as type two diabetes and obesity, has motivated researchers to investigate new enzyme inhibitors. Inhibition of the α-amylase enzyme is one therapeutic approach in lowering glucose levels in the blood to manage diabetes mellitus. The objective of this study was to synthesize short α-/β-mixed peptides in the solution phase. The Boc-protected α-L-leucine was converted to β-analogue by using Arndt–Eistert synthesis with the advantage of no racemization and retention of configuration. Three novel short peptides were successfully synthesized: N(Boc)-Gly-β-Leu–OCH3(14), N(Boc)-O(Bz)α-Ser-β-Leu–OCH3(16), and N(Boc)-O(Bz)-α-Tyr-α-Gly-β-Leu–OCH3(17), characterized by FTIR and 1H NMR analysis. The synthesized peptide 16 showed highest inhibitory activity (45.22%) followed by peptide 14 (18.51%) and peptide 17 (17.05%), respectively. Intriguingly, peptide 16 showed higher inhibition on α-amylase compared with other α-/β-mixed peptides.
Heteroleptic Complexes of Ruthenium Nitrosyl with Pyridine and Bypiridine—Synthesis and Photoisomerization
Authors: Brovko Anastasiya O. , Kuratieva Natalya V. , Pishchur Denis P. , Kostin Gennadiy A.
Published: August 2024

The reaction of [RuNO(Py)2Cl2OH] with bipyridine in water–ethanol media results in trans-(NO, OH)-[RuNO(Py)(Bpy)ClOH]+ with an acceptable yield (60–70%) as hexafluorophosphate salt. Further treatment of the hydroxy-complex with concentrated HF quantitatively leads to trans-(NO, F)-[RuNO(Py)(Bpy)ClF]+. Despite the chirality of both coordination spheres, the hexafluorophosphate salts crystallized as racemates. A NO-linkage isomerism study of the obtained complexes was performed at 80 K with different excitation wavelengths (405, 450, 488 nm). The most favorable wavelengths for the MS1 isomer (Ru-ON) formation were 405 and 450 nm, where the linkage isomer populations were 17% and 1% for [RuNO(Py)(Bpy)ClOH]PF6 and [RuNO(Py)(Bpy)ClF]PF6. The shift of the excitation wavelength to the green (488 nm) sharply decreased the MS1 population. The IR-spectral signatures of MS1 were registered. Reverse-transformation Ru-ON (MS1)-Ru-NO (GS) was investigated for [RuNO(Py)(Bpy)ClOH]PF6 using IR and DSC techniques that made it possible to determine the kinetic parameters (Ea and k0) and decay temperature.
Parasitological Examination of the Digestive System of Wild Boar from a Practical Point of View—Endoparasitological Sampling under Field Conditions
Authors: Farkas Csaba , Juhász Alexandra , Fekete Balázs , Egri Borisz
Published: August 2024

From 2015 to 2023, we conducted a comprehensive study in the 11,893-hectare hunting area managed by the Marcal-Bitvaközi Hunting Company, characterised by its substantial wild boar population. The research was carried out across various settings, including a free-range wild boar garden during large-scale hunts and free-living areas during individual hunts. We examined 216 wild boars in total, with 173 individuals from free-living areas and 43 from free-range areas. Throughout the sample collection process, we encountered numerous technical challenges that are infrequently detailed in the professional literature, often mentioned only tangentially. This oversight in existing publications neglects the significance of addressing field sampling difficulties, which are crucial for ensuring the precision and accuracy of research. This paper details the equipment requirements, sampling methodologies, and practical solutions to streamline fieldwork. While our primary focus was on endoparasitic infections of the stomach and small intestine, the described methodologies and findings are broadly applicable to research involving all internal organs.
Hormonal Influences on Skeletal Muscle Function in Women across Life Stages: A Systematic Review
Authors: Kodete Chandra Shikhi , Thuraka Bharadwaj , Pasupuleti Vikram , Malisetty Saiteja
Published: August 2024

Skeletal muscle function is vital for locomotion, posture, and metabolism, significantly impacting overall health and preventing falls, morbidity, and mortality, especially in elderly populations. This systematic review investigates the influence of hormonal fluctuations on skeletal muscle function across different life stages in women, including adolescence, the reproductive years, and menopause. A comprehensive literature search was conducted using databases such as PubMed, Scopus, and Web of Science to identify relevant studies. This review includes 45 studies that met the inclusion criteria, examining the roles of estrogen, progesterone, and other hormones in muscle metabolism, strength, and recovery. The findings highlight significant stage-specific hormonal impacts on muscle function, revealing how puberty, menstrual cycles, pregnancy, and menopause uniquely affect muscle health. Effective hormonal and non-hormonal interventions tailored to each life stage were identified, offering insights for optimizing muscle function and health management in women. This synthesis aims to bridge the gaps in understanding the hormonal regulation of muscle function, providing a foundation for future research and guiding clinical practices.
The Effect of Muscle Stretching on Joint Position Sense in Active Young and Elderly Adults: A Comparative Study
Authors: Haab Thomas , Leinen Peter , Stanek Madeleine
Published: August 2024

Previous studies have revealed decreased proprioception and perception of joint position in elderly adults. Joint position sense, indicating an individual’s ability to perceive the position of limbs without visual aid, is essential for everyday movements. A few studies have shown a positive effect of muscle stretching on joint position sense (JPS). However, these studies have only been conducted with younger participants. The impact of stretching on JPS in elderly adults remains unclear. Therefore, the aim of this study is to compare the acute effects of muscle stretching on joint position sense in young and elderly adults. An experimental group of younger adults (n = 15; 25.4 ± 2.9 years) and an experimental group of active, healthy elderly adults (n = 15; 64 ± 3.5 years) stretched their knee flexor muscles. The absolute error (AE) of the joint position sense was calculated before and after muscle stretching. The results indicated no significant difference in the AE between groups (p > 0.05) after the stretching intervention. The similarity in the physical activity status of the subjects may have influenced the results. Even though no significant age-specific differences were found in this study, its results may not be applicable to non-active elderly adults.
Effects of Substrate and Annealing Conditions on the Ferroelectric Properties of Non-Doped HfO2 Deposited by RF Plasma Sputter
Authors: Lim Seokwon , Ahn Yeonghwan , Won Beomho , Lee Suwan , Park Hayoung , Kumar Mohit , Seo Hyungtak
Published: August 2024

In this study, the effect of annealing and substrate conditions on the ferroelectricity of undoped hafnium oxide (HfO2) was analyzed. Hafnium oxide was deposited on various substrates such as platinum, titanium nitride, and silicon (Pt, TiN, Si) through RF magnetron sputtering. Annealing was performed in a nitrogen atmosphere at temperatures ranging from 400 to 600 °C, and the process lasted anywhere from 1 to 30 min. As a result, it was confirmed that the orthorhombic phase, the main cause of ferroelectricity, was dominant after a post-anneal at 600 °C for 30 min. Additionally, it was observed that interface mixing between hafnium oxide and the substrate may degrade ferroelectricity. Accordingly, the highest remanent polarization, measured at 14.24 μC/cm2, was observed with the Pt electrode. This finding was further corroborated by piezo force microscopy and endurance tests, with the results being significant compared to previously reported values. This analysis demonstrates that optimizing substrate and annealing conditions, rather than doping, can enhance the ferroelectricity of hafnium oxide, laying the foundation for the future development of ferroelectric-based transistors.
Improving the Stationary Entanglement of a Laguerre–Gaussian Cavity Mode with a Rotating Mirror via Nonlinear Cross-Kerr Interactions and Parametric Interactions
Authors: Lai Guilin , Huang Sumei , Deng Li , Chen Aixi
Published: August 2024

Quantum entanglement is essential in performing many quantum information tasks. Here, we theoretically investigate the stationary entanglement between a Laguerre–Gaussian (LG) cavity field and a rotating end mirror in an LG-cavity optorotational system with a nonlinear cross-Kerr (CK) interaction and a degenerate optical parametric amplifier (OPA). We calculate the logarithmic negativity of the system to quantify the stationary entanglement. We examine the influence of various system parameters such as the cavity detuning, the strength of the nonlinear CK interaction, the parametric gain and phase of the OPA, the power of the input Gaussian laser, the topological charge of the LG-cavity field, the mass of the rotating end mirror, and the ambient temperature on the stationary entanglement. Under the combined effect of the nonlinear CK interaction and the OPA, we find that the stationary entanglement can be substantially enhanced at lower Gaussian laser powers, smaller topological charges of the LG-cavity field, and larger masses of the rotating end mirror. We show that the combination of the nonlinear CK interaction and the OPA can make the stationary entanglement more robust against the ambient temperature.
Mechanism for Adsorption, Dissociation, and Diffusion of Hydrogen in High-Entropy Alloy AlCrTiNiV: First-Principles Calculation
Authors: Zheng Weilong , Wu Liangliang , Shuai Qilin , Li Zhaoqiang , Wang Haoqi , Fu Wei , Jiang Zhenxiong , Zhao Chuang , Hua Qingsong
Published: August 2024

To investigate hydrogen behaviors in the high-entropy alloy AlCrTiNiV, density functional theory and transition state theory were used to explore the molecular H2 absorption and dissociation and the atomic H adsorption, diffusion, and penetration progress. The H2 molecule, where the H-H band is parallel to the surface layer, is more inclined to absorb on the top site of the Ti atom site of first atomic layer on the AlCrTiNiV surface, then diffuse into the hollow sites, through the bridge site, after dissociating into two H atoms. Atomic H is more likely to be absorbed on the hollow site. The absorption capacity for atomic H on the surface tends to decline with the increase in H coverage. By calculating the energy barriers of atomic H penetration in AlCrTiNiV, it was indicated that lattice distortion may be one important factor that impacts the permeation rate of hydrogen. Our theory research suggests that high-entropy alloys have potential for use as a hydrogen resistant coating material.
Pd Catalysts Supported on Mixed Iron and Titanium Oxides in Phenylacetylene Hydrogenation: Effect of TiO2 Content in Magnetic Support Material
Authors: Talgatov Eldar T. , Naizabayev Akzhol A. , Bukharbayeva Farida U. , Kenzheyeva Alima M. , Yersaiyn Raiymbek , Auyezkhanova Assemgul S. , Akhmetova Sandugash N. , Zhizhin Evgeniy V. , Brodskiy Alexandr R.
Published: August 2024

Recently, Pd catalysts supported on magnetic nanoparticles (MNPs) have attracted a great attention due to their ability of easy separation with an external magnet. Modification of MNPs is successfully used to obtain Pd magnetic catalysts with enhanced catalytic activity. In this work, we discussed the effect of titania content in TiO2/MNPs support materials on catalytic properties of Pd@TiO2/MNPs catalysts in phenylacetylene hydrogenation. TiO2/MNPs composites were prepared by simple ultrasound-assisted mixing of TiO2 and MNPs, synthesized by co-precipitation method. This was followed by deposition of palladium ions on the mixed metal oxides using NaOH as precipitant. The supports and catalysts were characterized using XRD, BET, STEM, EDX, XPS, and a SQUID magnetometer. Pd nanoparticles (5–6 nm) formed were found to be homogeneously distributed on support materials representing the well-mixed metal oxides with TiO2 content of 10, 30, 50, or 70%wt. Testing of the catalysts in phenylacetylene hydrogenation showed that their activity increased with increasing TiO2 content, and the process was faster in alkali medium (pH = 10). The hydrogenation rates of triple and double C–C bonds on Pd@70TiO2/MNPs achieved 9.3 × 10−6 mol/s and 23.1 × 10−6 mol/s, respectively, and selectivity to styrene was 96%. The catalyst can be easily recovered with an external magnet and reused for 12 runs without significant degradation in the catalytic activity. The improved catalytic properties of Pd@70TiO2/MNPs can be explained by the fact that the surface of the support is mainly composed of TiO2 particles, affecting the state and size of Pd species.
Exploring the Geroprotective Potential of Nutraceuticals
Authors: Rivero-Segura Nadia Alejandra , Zepeda-Arzate Emmanuel Alejandro , Castillo-Vazquez Selma Karime , Fleischmann-delaParra Patrick , Hernández-Pineda Jessica , Flores-Soto Edgar , García-delaTorre Paola , Estrella-Parra Edgar Antonio , Gomez-Verjan Juan Carlos
Published: August 2024

Aging is the result of the accumulation of a wide variety of molecular and cellular damages over time, meaning that “the more damage we accumulate, the higher the possibility to develop age-related diseases”. Therefore, to reduce the incidence of such diseases and improve human health, it becomes important to find ways to combat such damage. In this sense, geroprotectors have been suggested as molecules that could slow down or prevent age-related diseases. On the other hand, nutraceuticals are another set of compounds that align with the need to prevent diseases and promote health since they are biologically active molecules (occurring naturally in food) that, apart from having a nutritional role, have preventive properties, such as antioxidant, anti-inflammatory and antitumoral, just to mention a few. Therefore, in the present review using the specialized databases Scopus and PubMed we collected information from articles published from 2010 to 2023 in order to describe the role of nutraceuticals during the aging process and, given their role in targeting the hallmarks of aging, we suggest that they are potential geroprotectors that could be consumed as part of our regular diet or administered additionally as nutritional supplements.
Vitamin D and Gestational Diabetes Mellitus in the IEU OpenGWAS Project: A Two-Sample Bidirectional Mendelian Randomization Study
Authors: Bai Yuxuan , Wang Xiaoxiao , Xu Yaxuan , Jiang Chang , Liu Haoran , Xu Zixiu , Shen Jinping , Zhang Xumei , Zhang Qiang , Du Yue
Published: August 2024

Background: Gestational diabetes mellitus (GDM) is one of the most prevalent pregnancy problems, and there is still debate over the relationship between vitamin D and GDM. Objectives: Our objective is to investigate the correlation between vitamin D and GDM by employing Mendelian randomization (MR) with summary data obtained from genome-wide association studies (GWAS). Methods: Data on exposures and outcomes, namely vitamin D, vitamin D insufficiency, and GDM, were acquired from the IEU OpenGWAS Project. Bidirectional MR analysis was performed utilizing the inverse variance weighted (IVW) method as the principal analytical approach. The complementary approaches employed in this study encompassed weighted median, simple mode, weighted mode, and MR-Egger regression. A series of sensitivity analysis were conducted in order to assess the reliability of the obtained results. Results: The data were acquired from the IEU OpenGWAS Project. Following the application of the three assumptions of MR, 13 single nucleotide polymorphisms (SNPs) were included in the MR analysis for vitamin D levels and vitamin D deficiency on GDM, and 10 and 26 SNPs were included for GDM on vitamin D levels and deficiency, respectively. The findings from the IVW analysis revealed a significant positive correlation between vitamin D levels and GDM (OR = 1.057, 95% CI: 1.011–1.104, p = 0.015). Conversely, a negative correlation was seen between vitamin D deficiency and GDM (OR = 0.979, 95% CI: 0.959–0.999, p = 0.039). The results of the reverse MR study revealed no evidence of reverse causation between GDM and vitamin D. The findings from multiple MR approaches were in line with the direction of IVW analysis. Sensitivity analysis revealed no evidence of heterogeneity, pleiotropy, or outliers, suggesting the robustness of the results. Conclusions: There exists a causal association between vitamin D and GDM, whereby vitamin D levels serve as a risk factor for GDM.
Single-Person Households: Insights from a Household Survey of Fruit and Vegetable Purchases
Authors: Silva Andres , Rivera Maripaz , Durán-Agüero Samuel , Sactic Maria Isabel
Published: August 2024

Despite the efforts made to promote consumption, some countries are not increasing their fruit and vegetable intake, while household structures are undergoing relevant changes. Fruit and vegetable consumption is necessary but not sufficient for a healthy diet. Previous research has linked adequate fruit and vegetable consumption to a lower risk of cardiovascular diseases, type 2 diabetes, and some mental health conditions. Furthermore, millions of deaths are reported annually worldwide due to diets low in fruit and vegetables, highlighting their critical public health importance. This study aims to separately analyze the purchases of fruit and vegetables in single-person households. We used three waves of the Family Budget Survey, Encuesta de Presupuestos Familiares, in Chile, which is nationally representative of urban areas and includes over 10,000 households in each wave. We employed descriptive statistics to examine the characteristics of the head of household and the food shopper as well as the structure, composition, and overall characteristics of households. Additionally, we performed separate analyses for fruit and vegetable purchases, using these variables to determine the marginal effect on the probability of purchasing fruit or vegetables through probit models. Results show that, from 2011–2012 to 2021–2022, the share of households not purchasing fruit and vegetables increased from 5.0% to 8.4% and that, in single-person households, it rose from 11.2% to 19.1%. Male-headed, single-person households with low education and income were more likely not to purchase fruit, and these households also have decreasing vegetable purchases. Additionally, household income significantly impacts fruit purchases but does not significantly affect vegetable purchases. Our findings highlight the importance of considering single-person households as a target population segment for future public policies to promote fruit and vegetable consumption.
Nutritional Assessment in Outpatients with Heart Failure
Authors: López Guillén Regina , Argente Pla María , Micó García Andrea , Dura de Miguel Ángela , Gascó Santana Eva , Martín Sanchis Silvia , Merino Torres Juan Francisco
Published: August 2024

Introduction: Heart failure (HF) is associated with significant alterations in body composition, including malnutrition due to insufficient intake, chronic inflammation and increased energy expenditure. Identifying the prevalence of malnutrition and the risk of sarcopenia in patients with HF is crucial to improve clinical outcomes. Material and methods: This cross-sectional, single-center, observational study involved 121 outpatients diagnosed with HF. Nutritional status was assessed using the Mini Nutritional Assessment (MNA), the Malnutrition Universal Screening Tool (MUST), and the Subjective Global Rating (SGA). Sarcopenia was screened using the SARC-F (Strength, Assistance in walking, Rise from a chair, Climb stairs, Falls) questionnaire and diagnosed based on the European Working Group in Older People (EWGSOP2) criteria and functionality with the Short Performance Battery (SPPB) test. Malnutrition was diagnosed according to the Global Leadership Initiative on Malnutrition (GLIM) criteria. Results: The study found that 10.7% had cardiac cachexia and 45.4% of deceased patients had been in this condition (p = 0.002). Moderate-to-high risk of malnutrition was identified in 37.1%, 23.9%, and 31.4% of patients according to the MNA, MUST, and SGA tests, respectively. According to the GLIM criteria, 56.2% of patients were malnourished. Additionally, 24.8% of patients had a high probability of sarcopenia, and 57.8% were not autonomous according to SPPB. Patients with less than 30% quadriceps muscle contraction were at a high risk of sarcopenia. Conclusions: There is a high prevalence of malnutrition among outpatients with HF, which is associated with worse prognosis, increased risk of sarcopenia, and greater frailty. These findings underscore the importance of early nutritional and functional assessments in this population to improve clinical outcomes.
In Vivo Evaluation of Sepigel-Based Meglumine Antimoniate and Amphotericin B for Cutaneous Leishmaniasis Treatment
Authors: López-Arencibia Atteneri , Bethencourt-Estrella Carlos J. , Berenguer Diana , Domínguez-de-Barros Angélica , Alcover M. Magdalena , Sessa Marcella , Halbaut Lyda , Fisa Roser , Calpena-Campmany Ana Cristina , Córdoba-Lanús A. Elizabeth , Lorenzo-Morales Jacob , Riera Cristina , Piñero José E.
Published: August 2024

Cutaneous leishmaniasis (CL) poses a significant public health concern in endemic regions due to its increasing prevalence and substantial impact on affected individuals. This disease is primarily caused by the Leishmania protozoa, which are transmitted through insect bites, and it manifests as a range of symptoms, from self-healing lesions to severe disfigurement. Current treatments, which often involve the parenteral administration of antimonials, face challenges such as poor compliance and adverse effects. This study investigates the efficacy of topical formulations containing meglumine antimoniate (MA) and amphotericin B (AmB), using Sepigel as an excipient, for treating CL. In the in vivo study, BALB/c mice infected with L. amazonensis developed lesions at the injection site five weeks post-infection. Subsequently, the mice were divided into eight groups: untreated mice, mice treated orally with miltefosine, mice treated intraperitoneally with MA, and mice treated topically with 15%, 22.5%, and 30% MA-Sepigel, as well as those treated with AmB-Sepigel. Treatments were applied daily for two weeks, and the results revealed a significant reduction in lesion size and parasite burden following topical application, particularly with the AmB-Sepigel formulations and 30% MA-Sepigel. Additionally, Sepigel-based treatments demonstrated improved patient compliance and reduced toxicity compared to systemic therapies. These findings underscore the potential of Sepigel-based formulations as a promising alternative for CL treatment. They offer enhanced efficacy and tolerability, while reducing the systemic toxicity associated with conventional therapies.
First Molecular Evidence of Babesia caballi and Theileria equi in Imported Donkeys from Kyrgyzstan
Authors: Wu Xuanchen , Xu Jun , Su Lixin , Li Ente , Wang Suwen , Hornok Sándor , Liu Gang , Wang Yuanzhi
Published: August 2024

Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi, Theileria haneyi, and Babesia caballi. Nonetheless, there has been a scarcity of systematic reports on EP parasites in donkeys in Kyrgyzstan, Central Asia. In this study, piroplasms were screened in 1900 blood samples from imported donkeys from the Osh Oblast (southwestern Kyrgyzstan) by targeting partial 18S ribosomal RNA using the polymerase chain reaction (PCR). Through molecular and phylogenetic analyses, all positive samples were sequenced to identify the species and genotypes. The results indicated the presence of both B. caballi and T. equi, with prevalence rates of 8.4% (160/1900) and 12.2% (232/1900), respectively. By amplifying part of the Erythrocyte Merozoite Antigen 1 (EMA-1) and Rhoptry-Associated Protein (RAP-1) genes, B. caballi genotype B and T. equi genotype A were identified. To the best of our knowledge, this is the first report on piroplasm infection among donkeys from Kyrgyzstan.