bluuebunny/crossref_metadata_2025 huggingface dataset:
APLIKASI PENDAFTARAN EKSTRAKURIKULER BERBASIS WEB DENGAN METODE WATERFALL
Authors: Mulyanto Yudi , Susanto Eri Sasmita , Ilyas Muhammad
Published: June 2023

SMAN 1 Moyo Utara merupakan salah satu institusi pendidikan yang didalamnya terdapat pengembangan potensi dan bakat siswa berupa kegiatan ekstrakurikuler. Untuk mengikuti kegiatan tersebut para siswa diharuskan mendaftar terdahulu ekstrakurikuler yang diminati. Pendaftaran masih dilakukan secara manual menggunakan formulir pendaftaran. Setelah itu formulir tersebut di rekap satu per satu dengan menulis kembali ke dalam blangko pendaftaran. Hal tersebut dinilai sangat kurang efisien dan efektif. Berdasarkan permasalahan tersebut peneliti melakukan pendekatan kualitatif dan metode yang digunakan adalah metode pengembangan perangkat lunak waterfall, maka dibuatlah aplikasi berbasis website untuk proses pendaftaran ekstrakurikuler tersebut. Aplikasi di buat menggunakan bahasa pemrograman PHP dengan framework Codeigniter dan database Maria DB. Manfaat dari aplikasi ini adalah untuk membantu siswa untuk mendaftar ekstrakurikuler dalam mengurangi penggunaan kertas yang berlebihan dapat di atasi dan pembina tidak harus merekap satu persatu data yang masuk.
A Thick Green Line: Extracting Public Space from Infrastructure
Authors: Atkinson Cheryl
Published: June 2023

The speed and intensity of the current residential high-rise development of Toronto’s downtown core is unprecedented in North America. While this new population has significantly rejuvenated the street life and economy of this area, the public space to support it remains severely inadequate in quality, extent, and connectivity. This design proposal looks at the opportunities available to extract new public domain from existing remnant infrastructure within the historic core. By analyzing the urban design history of this area, and current pedestrian and traffic patterns, a new intervention is proposed that re-allocates space from cars to people. This proposal creates new amenity space and links a number of vestigial and derelict historic public squares into a larger comprehensive system. The proposed linear park establishes a needed cultural, civic, and community focus and encourages walking and cycling for this live-work population. The design is made present by a hyper-articulation of its horizontal surface—a repetitive and continuous paving with a memorable pattern and form. This strategy is a pragmatic but aggressive approach that recognizes the criticality of extracting a pedestrian realm within the emerging hyper-density of this particular car-oriented city. It makes use of available historic patterns, and existing redundant traffic infrastructure, to create place, meaning, and amenity.

 
ZERO HOUS[ING] 1:1 Prototype + Process: Collaborative and Experiential Education in the Global Housing and Climate Crisis
Authors: Atkinson Cheryl
Published: June 2023

Zero Hous[ing] is a recently built energy neutral midrise housing prototype that investigated alternative sites, and alternative forms of practice and production. It used prefabrication and a carbon sequestering palette to address the housing affordability and climate crisis. While it was produced and reads as a single family house— it was designed to work as housing for metropolitan areas. This project considers urban typology, architectural design for aesthetics, function, health and well-being, and innovative construction methodologies to look at this problem from the bottom-up and across sectors. The objective was to build demand with consumers and industry for net-zero energy and carbon sequestering housing by making a healthy and attractive architecture, creating site location and construction efficiencies, and demonstrating through this built prototype and its life-cycle cost and energy analysis, that it might be accessible to the many rather than the few. We realized this building with custom prefabrication and a deeply integrated design process engaging a cross-disciplinary team of professionals, educators, builders and students in all stages of the work. This project is remarkable for the ambition and scope of its definition of sustainable design (urban design, carbon footprint, energy use and construction methodology) and for its recognition that, in order to change existing paradigms we need to actively interact, and through experiments like this, develop new ways to design, build, and live collaboratively. This educational project integrated architecture, engineering, and business faculty and students at Ryerson University, Toronto Canada and an industry construction/education partner called The Endeavour Centre. We collaborated on this one-to-one scale prototype using Passive House principles, and prefabrication as an ethic. We built it using our industry partner’s team of apprentice carpenters and it is now being enjoyed as a full time residence for its owner while we monitor its performance.
Worry about professional education: Emotions and affect in the context of neoliberal change in postsecondary education
Authors: Moffatt Ken , Todd Sarah , Barnoff Lisa , Pyne Jake , Panitch Melanie , Parada Henry , McLeod Sandy , Young Nataleah Hunter
Published: June 2023

A growing literature deals with the role of emotions and affect in making sense of how neoliberal changes in governance are experienced. In our study of the effects of neoliberal changes within Canadian universities, we discovered that faculty members are experiencing emotional as well as cognitive responses, to these changes. This paper aims to contribute to the understanding of how neoliberalism is embodied in emotion, shaping the spaces in which we are located. The ways in which neoliberal ideology reorganizes workplaces results in people experiencing a range of affects that are then displayed in the form of emotions. We consider how space shapes affect and, in turn, emotional responses to neoliberal change. These emotions generate meaning and work as a form of power that aligns bodies with, and separates them from, others (Ahmed, 2004a).
Vacant City: AR 8101 Studio in Critical Practice
Authors: Floerke Paul , Atkinson Cheryl , Cleland Christopher , Gu Jessica , Kong Wincy , Maidankine Christian , Sabzevari Sam , Culmone Caleb , D'Penna Kristen , Ene Ana , Giannetti Joe , Ho Clark , Kang Steve , Kosiechenko Anna , Oh Anna , Rosano Bernadette , Shahmooradian Chantal , Tahir Nuvaira , Tandazo Jessica
Published: June 2023

What does 'Vacancy' mean in each of these cities of contested and valuable space? What are the various forms of vacancy? What are the forces producing and eliminating 'vacancies' historically? What opportunities and challenges are presented by the 'absence' of architecture?
“The fight is two times as hard”: A qualitative examination of a violence syndemic among young Black sexual minority men
Authors: Quinn Katherine G. , Edwards Travonne , Johnson Anthony , Spector Antoinette L. , Takahashi Lois , Dakin Andrea L. , Bouacha Nora , Valadez-Tapia Silvia , Voisin Dexter
Published: June 2023

Young Black men who have sex with men (YBMSM) are disproportionately impacted by violence, including violence rooted in anti-Black racism, sexual identity bullying, and neighborhood violence rooted in structural racism and inequities. These multiple forms of violence are frequently co-occurring and interactive creating syndemic conditions that can negatively impact HIV care. This qualitative study is based on in-depth interviews with 31 YBMSM, aged 16–30 years, living with HIV in Chicago, IL, to examine how violence has impacted their lives. Using thematic analysis, we identified five themes that reflect how YBMSM experience violence at the intersection of racism, homonegativity, socioeconomic status, and HIV status: (a) the experience of intersectional violence; (b) long histories of violence contributed to hypervigilance, lack of safety, and lack of trust; (c) making meaning of violence and the importance of strength; (d) normalizing violence for survival; and (e) the cyclical nature of violence. Our study highlights how multiple forms of violence can accumulate across an individual’s life and contribute to social and contextual situations that further contribute to violence and negatively impact mental health and HIV care.
COVID‑19, Retention in HIV Care, and Access to Ancillary Services for Young Black Men Living with HIV in Chicago
Authors: Voisin Dexter , Edwards Travonne , Takahashi Lois , Valadez-Tapia Silvia , Shah Habiba , Oselett Carter , Bouacha Nora , Dakin Andrea L. , Quinn Katherine G.
Published: June 2023

This study conducted 28 semi-structured, in-depth interviews with Young Black Men who have Sex with Men in Chicago to investigate the impact of COVID-19 on their HIV care and ancillary service access. The qualitative analysis identified both negative and positive effects. The negative effects included: (l) mixed disruptions in linkage to and receipt of HIV care and ancillary services, and (2) heightened concerns about police and racial tensions in Chicago following the murder of George Floyd, contributing to possible disruption of retention in care. The positive effects included: (1) the ability to reflect and socially connect, contributing to heightened self-care and retention in care, and (2) some improvements in receipt of medical care. These findings suggest that while COVID-19 disruptions in care reduced in-person use of HIV care, the expansion of telemedicine allowed more administrative tasks to be handled online and focused in-person interactions on more substantive interactions.
Civilian perceptions of police: A thematic analysis of non-physical encounters with law enforcement
Authors: Edwards Travonne , Sharpe Tanya L. , Sibblis Camisha , McPolland Megan , Bonomo Antonia , DeVylder Jordan
Published: June 2023

Literature pertaining to civilian-police relations within the United States primarily focuses on unjust physical treatment of civilians by law enforcement. However, research examining the ways in which adverse nonphysical encounters with law enforcement influence perceptions of police and compromise their relationship with civilians is less prevalent. This study utilizes a thematic approach to analyze 252 participants open-ended responses from the Police–Public Encounters survey. The Police–Public Encounters survey was designed to investigate the prevalence, demographic distribution, and psychological correlates of police victimization from adults across four US cities (Baltimore, New York, Philadelphia and Washington, DC), to understand their most notably adverse encounter with police. Study findings revealed four themes: 1) direct and indirect experiences of racial profiling, 2) fear and intimidation, 3) unjust treatment, and 4) poor quality of service. Findings highlight the relationship between nonphysical encounters with police and notions of procedural justice that influence civilian-police interactions. Implications for future research should continue to explore citizens’ perceptions of police as well as police perceptions of their encounters with civilians to examine how this may affect their ability to serve and protect communities.
AS NOVAS ENCRUZILHADAS DO TRABALHO PORTUÁRIO EM TEMPOS DE PANDEMIA
Authors: Queiroz Maria de Fátima , Barros Thiago Pereira de , Nunes Leandro , Lara Ricardo , Nunes João Renato Silva Nunes
Published: June 2023

A pandemia levou a população mundial a adotar regras de isolamento social e normas de segurança que têm por objetivo “impedir” a disseminação do vírus. Nessa condição pandêmica, o trabalho, como central para reprodução do modo capitalista de produção foi severamente afetado pela impossibilidade de se manter nos moldes anteriores ao processo pandêmico. Trabalhadores foram declarados como essenciais, incluindo os portuários. Assim, este artigo tem como objetivo refletir sobre o trabalho portuário no contexto da pandemia de Covid-19. O artigo apresenta um breve contexto do trabalho portuário em nível mundial e suas respectivas realidades frente à pandemia e, por conseguinte, o trabalho portuário no Brasil, suas transformações históricas sucedidas com a adoção das políticas neoliberais, e finaliza abordando a situação dos trabalhadores portuários brasileiros em tempos de pandemia. As considerações finais indicam que mesmo o país passando por um dos momentos mais críticos de sua história, imerso numa crise de saúde pública, o interesse com a saúde e condições de trabalho dos portuários não é a pauta no meio empresarial e do Estado. Nesse cenário, os trabalhadores portuários têm no horizonte um caminho ainda mais intenso de luta e, portanto, de recomposição de seu trabalho nos portos nacionais.
concept of jihad for education
Authors: Hoque Mesbahul , Jaim Mohammad Syazri , Mohamed Yuslina
Published: June 2023

Jihad has a very broad context that encompasses many fields, each with its own definition. The term Jihad is also widely used by Muslims for a positive purpose that is to strive for good to gain the pleasure of God. However, non-Muslims and even some Muslims have a misconception about the true meaning of jihad, conflating it with a violent struggle to uphold one's religious beliefs. In addition, many Muslims and non-Muslims alike give a limited definition of jihad, claiming that it only refers to "Holy War". Therefore, this study will examine the concept of Jihad in general through the views of reliable scholars. In addition, the researcher will specifically examine the merits of Jihad in seeking knowledge based on the arguments of the Quran and Sunnah that are appropriate for this Jihad seeking knowledge. This study will use a data-based library which the descriptive analysis of the text will be used as the main mechanism to analyze the content of the text to collect data related to the concept of Jihad. This analysis takes into account the opinions and views of the scholars that they conclude based on the Quran and Sunnah in their books. This study demonstrates that seeking knowledge is a component of Jihad, which is highly demanded by Islam. Furthermore, the study relating to the concept of Jihad demonstrates that Jihad has a very broad scope, encompassing Jihad against lust, the devil, with property for the sake of Islam, and more.
Keberkesanan aplikasi Wordwall dalam pengajaran dan pembelajaran gaya bahasa seloka boros dan jimat bagi pelajar tingkatan 4
Authors: Zakaria Norazimah , Anak Sili Evelyn
Published: June 2023

Kajian “Keberkesanan Aplikasi Wordwall dalam Pengajaran dan Pembelajaran Gaya Bahasa Seloka Boros dan Jimat bagi Pelajar Tingkatan 4” menumpukan kepada fungsi atau kelebihan teknologi ini dalam membantu guru menjalankan pengajaran dalam kelas. Kajian ini dilaksanakan dengan menggunakan Teori Kecerdasan Pelbagai (Verbal-Linguistik). Kajian terdiri daripada dua objektif iaitu pertama melihat kepada fungsi dan kelebihan aplikasi Wordwall dalam pengajaran dan pembelajaran gaya bahasa Seloka Boros dan Jimat. Objektif kedua pula melihat kepada keberkesanan penggunaan aplikasi ini dalam sesi pengajaran dan pembelajaran dalam kelas berdasarkan Teori Kecerdasan Pelbagai (Verbal-Linguistik). Kajian ini melibatkan tujuh responden yang terdiri daripada dua orang guru dan lima orang pelajar Tingkatan Empat yang mengambil subjek Kesusasteraan Melayu. Kajian ini merupakan kajian kualitatif iaitu melalui kaedah deskriptif, temu bual, pemerhatian dan analisis dokumen. Data kajian dikumpul dengan membuat sesi temu bual bersama dua orang guru, pemerhatian berdasarkan senarai semak serta menganalisis lembaran kerja yang telah disiapkan oleh pelajar. Analisis daripada dapatan kajian mendapati bahawa guru menggalakkan penggunaan teknologi dalam pengajaran subjek Kesusasteraan Melayu bagi meningkatkan kefahaman pelajar dalam sesebuah topik yang diajar. Kefahaman pelajar juga dapat dilihat dengan jelas sebelum dan selepas penggunaan aplikasi Wordwall dalam pengajaran gaya bahasa Seloka Boros dan Jimat. Terbukti bahawa pelajar lebih menyukai dan memahami isi pembelajaran hasil daripada lembaran kerja yang diberi. Pelajar juga dilihat menerapkan teori Verbal-Linguistik semasa sesi pembelajaran berlaku sama ada secara lisan atau tulisan. Melalui dapatan kajian diharapkan agar dapat membantu dalam meningkatkan kualiti pengajaran guru di dalam kelas.
Theoretical principles of restoration of the competitiveness of territorial communities and regions
Authors: Podzizei Oleh , Shmatkovska Tetiana
Published: June 2023

The article examines the theoretical aspects of ensuring the economic growth of territorial communities and regions of Ukraine. The set of problems that arise in certain regions and territories and negatively affect the dynamics of their socio-economic development is analyzed. The necessity of forming conceptual strategies for restoring the competitiveness of territorial communities in modern conditions is suggested. Approaches to understanding the essence of the concept of territorial competitiveness and its importance for the formation of territorial economic development plans are considered. Factors that directly affect the level of competitiveness of the regions of Ukraine are studied, among the key ones are the level of infrastructure development, the level of technological development of the region, the quality of the human capital of the region, etc. The problems that arise in regions and territorial communities, and which have a critical impact on the state of their competitiveness, are identified. A number of destructive factors have been identified that, under the influence of negative market dynamics formed as a result of the war, reduce the current state of competitiveness of territorial communities. Such factors include the lack of proper infrastructure, shortage of financial and material resources, decrease in the quality of life of the population, and others. The necessity of forming conceptual strategies for restoring the competitiveness of territorial communities and regions of Ukraine in the process of post-war development of the national economy was determined. Ways of improving the development of regional infrastructure through specialized targeted state programs, raising the level of education and professional training of the population, attracting investments on the basis of private-state partnership, etc. are proposed. The importance of continuing institutional changes in the field of local self-government to ensure the effective restoration of the competitiveness of regions and territorial communities has been proven. A set of theoretical provisions regarding the provision of solutions to the problems of socio-economic development of territorial communities and regions on the basis of ensuring the restoration of their competitiveness through the implementation of projects of regional significance on the basis of sustainable development are considered.
Investment potential of Ukraine in the conditions of war
Authors: Viblyi Petro , Blavt Anna
Published: June 2023

The article examines the issue of Ukraine's investment potential in wartime conditions. It was determined that considering the complex macroeconomic environment and the slowdown of the world economy, it is problematic to outline investment strategies in Ukraine in the conditions of war. It has been studied that today investments in Ukraine are mostly associated with financial instruments that allow attracting and directing capital to enterprises. The reasons that reduce Ukraine's investment attractiveness are analyzed, in particular, a high level of corruption and an insufficient regulatory framework. A number of measures are proposed to reduce these risks and ensure the stability of the investment climate in Ukraine. It has been established that Ukraine currently offers all interested parties a wide investment menu of potential opportunities worth more than $400 billion as part of the Advantage Ukraine campaign, which was launched in 2022 to attract potential foreign investors. With the support of the President of Ukraine, a special platform was created, where investment projects and opportunities are collected in 10 industries that have significant investment potential in the conditions of war in Ukraine. It has been studied that the extremely high level of adaptability of Ukrainian enterprises in the current situation of martial law ensures the formation of investment potential in 10 sectors: the defense-industrial complex ($43 billion), energy ($177 billion), logistics and infrastructure ($123 billion), agro-industrial complex ($34 billion), industrial production ($16 billion), innovative technologies ($11 billion), metallurgy and metalworking ($26 billion), pharmaceuticals ($19 billion), natural resources ($5.6 billion), woodworking and furniture production ($5 billion). It has been investigated that, taking into account the continuation of the war on the territory of Ukraine, the most important thing in the process of formation of investment potential is the assessment of the attractiveness of investing in a certain area as a source of profit on the invested capital, taking into account the guarantees of the security of investments in wartime and postwar times. According to the results of the conducted reconnaissance, it was established that Ukraine has a powerful investment potential, and therefore, broad prospects for attracting foreign investments even during a bloody and destructive war. Despite the military conflict, Ukraine has significant investment potential in sectors such as IT, agriculture, energy and tourism.
International labor migration and its impact on the labor market of Ukraine in the context of Russian military aggression
Authors: Bobrova Darіa , Don Olha
Published: June 2023

The modern development of the global economy is characterized by globalization, the tendency of economies towards openness, free movement of factors of production, including labor. The article discusses the phenomenon of international labor migration as the cross-border movement of individuals for the purpose of further employment. It is noted that it occurs under the influence of a range of interconnected factors, in conditions of growing differences between countries. The article analyzes the impact of mass labor migration of Ukrainians on the national labor market during the full-scale russian aggression, which the author considers as a crisis and destructive condition. It is proved that the unsatisfactory state of the labor market under the influence of crisis phenomena can also encourage migrants to leave and find employment in other countries. The conclusion is made that international labor migration and the national labor market are definitely interconnected and influence each other. It is noted that the consequences of international labor migration are ambiguous and have an impact on both the recipient and donor country. In particular, international labor migration can cause such obvious negative consequences for the national labor market as increased competition, lower overall wages in the receiving country, and brain drain in the country of origin. Using the example of Ukraine during the full-scale russian invasion, a labor shortage on the labor market due to the migration of workers abroad is noticed. It is emphasized that with appropriate comprehensive regulation, international labor migration becomes a real tool for improving the situation on national labor markets and has a positive impact on the well-being of states even in a crisis period. For example, foreign workers specially recruited from abroad or returning Ukrainian migrants can help overcome labor shortages in sectors where there is a significant lack of workforce Therefore, the necessary conditions for the formation of an effective migration policy during crisis conditions are: the study of current trends in international labor migration, the identification of the reasons that cause these trends, and the consequences of the impact of this phenomenon on the labor market in Ukraine.
دور العنوان الخبري التلفزيوني في جذب المشاهدين للنشرات الإخبارية
Authors: حسين يوسف محمد
Published: June 2023

يهدف البحث الى تحديد دور العنوان الخبري في جذب المشاهدين للنشرات الاخبارية، وكذلك أسباب ارتباط العنوان الخبري بمدى تقبل أو رفض الجمهور للخبر الصحفي المصاغ، والمقدم إليهم عبر تلك النشرات الاخبارية. عن طريق تحديد آليات كتابة وتحرير وطبيعة العنوان الصحفي من حيث الاسلوب والصياغة الماهرة والمشوقة. والتي بدورها تنعكس على تقبل وشد انتباه جمهور المشاهدين للخبر الصحفي عبر النشرات الاخبارية. فقد استخدم الباحث المنهج الوصفي، فضلاً عن توزيع استمارة الاستبانة بواقع 200 استمارة لفحص واستكشاف أفكار واتجاهات الجمهور بشأن موضوع البحث. وأسفرت الدراسة عن مجموعة من النتائج الرئيسة، منها: أن الجمهور يظهر اهتمامًا كبيرًا بمتابعة العناوين الرئيسة للنشرات الإخبارية، كما يُعدّ العنوان الصحفي عنصرًا هامًا يجذب الجمهور ويجعله يتابع النشرات الإخبارية. ويتأثر اهتمام الجمهور بالعناوين الصحفية بطريقة صياغتها وكتابتها بأسلوب تشويقي ومثير للاهتمام من قبل المحرر الصحفي.
Experimental and Numerical Comparative Study on RC Beam Flexurally Strengthened with CFRP Laminate.
Authors: Basit Md Abdul , Amin Md Tanvir Ehsan
Published: June 2023

In this article finite element model is prepared for reinforced concrete beam which is flexurally held together by means of carbon fiber reinforced polymer laminate. The 3D analysis is conducted using ABAQUS software and the results are investigated, where maximum load, deflection at mid span and strain characteristics of both reinforcement and concrete are analyzed numerically. Later these parameters are investigated experimentally in a similar way by keeping all the important conditions such as loading, reinforcement detailing, boundary condition etc. identical. This comparative assessment illustrates that, the using of CFRP has a significant advantage to improve flexure behavior of RC beams. Various types of failure mode such as deflection and stress for whole cross section and for only reinforcement are achieved from finite element analysis. In addition, typical deflection, stress and strain contours of a specified node are found for unstrengthen and CFRP strengthened beam. This article also represents both the reinforcement strain values and concrete strain values for strengthened and unstrengthen RC beams and found a precise similarity while comparing with finite element modelling results. Furthermore, in terms of ultimate load, the deviation in result is as narrow as 4% for flexurally strengthened reinforced concrete beam by CFRP laminate.
IUBAT Review—A Multidisciplinary Academic Journal, 6 (1): 13-24
Physiological causes of yield variation in similar durated mungbean genotypes
Authors: Mondal M Monjurul Alam , Haque Md Azadul , Islam Md Mohimenul
Published: June 2023

The experiment was conducted during February to May, 2019 at the research farm of Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202. The objective of the study was to evaluate the growth, reproductive characteristics, yield and yield components of four similar durated mungbean genotypes, namely BARI Mung-2, Binamoog-2, MB-29, and MB-43. At most of the growth stages, the mutant MB-29 showed superiority in terms of growth parameters such as total dry matter, leaf area index, absolute growth rate, relative growth rate, and net assimilation rate whilst MB-43 showed the inferiority. Furthermore, MB-29 outperformed the other three genotypes in yield attributing features, resulting in a higher seed yield than the other three genotypes. In comparison, the MB-43 mutant showed the lowest value for the yield parameters. The seed yield correlated positively and significantly with leaf area, total dry matter, absolute growth rate, and number of pods plant-1.
J. Bangladesh Acad. Sci. 47(1); 101-108: June 2023
Jute endophyte Grammothele lineata SDL-CO-2015-1 produces an anticancer compound epothilone B
Authors: Beg Mirza Ashikul , Hannan Sumaiya Binte , Rabeya - , Talukder Shafi Ahmad , Uddin Aftab , Akter Shammi , Reza Rifath Nehleen , Khan Haseena , Islam Mohammad Riazul
Published: June 2023

Jute (Corchorus olitorius) endophytic fungi Grammothele lineata SDL-CO-2015-1 was found to produce epothilone B, a well-reported anticancer compound. Both the genome and RNA-seq data of G. lineata revealed the presence of epothilone gene clusters. In silico mining using NaPDoS (Natural Product Domain Seeker) identified four genes matching with the epothilone gene cluster (Epo D). Epothilone was extracted from the extracellular extract of this fungus using XAD-16 resin. Different analytical techniques including thin layer chromatography (TLC), reverse phase high-performance liquid chromatography (RP-HPLC), and mass spectrometry (MS) were used to identify and characterize epothilone following its extraction. TLC bands were visible at 365 nm for both standard epothilone B and samples with identical Rf values. Extracted epothilone bands from the TLC plate were further purified using RP-HPLC. Using a linear gradient system with methanol and 0.1% formic acid, LC-MS spectroscopy was done with RP-HPLC purified fraction. The monoisotopic mass of epothilone (M+H+) = 508.1 Da was present in the mass chromatogram. The product ions of 508.1 Da were found with the m/z values at 320.2 Da, and 420.2 Da, the typical MS2 fragments for standard epothilone B. This finding suggests that the fungal endophyte, G. lineata can be a potential source of epothilone B.
J. Bangladesh Acad. Sci. 47(1); 91-99: June 2023
Computational analysis of functional single nucleotide polymorphisms associated with NPAS2 gene
Authors: Khan Sumaiya Farah , Rima Fahmida Sultana , Hassan Fuad
Published: June 2023

Circadian rhythm is a natural physiological process that regulates the sleep-wake cycle and occurs about every 24 hours. The fundamental molecular clock, NPAS2 (Neuronal PAS Domain Protein 2), generates and regulates mammalian circadian rhythms. Various diseases have been linked to single nucleotide polymorphisms (SNPs) in NPAS2. In this study, several computational approaches have been employed to predict the SNPs' functional and structural consequences and investigate the deleterious roles of nsSNPs. Two deleterious nsSNPs (F190L and S105I) were found with predicted functions linking to disease. Alongside, from the obtained ConSurf result, Q73, E61, K51, E167, L317, and R514 were expected as highly conserved and exposed, while T114, T328, and C299 were predicted as highly conserved and buried. However, to establish their role in disease pathogenesis, further studies need to be done on the deleterious mutations of the NPAS2 gene.
J. Bangladesh Acad. Sci. 47(1); 53-60: June 2023
Aktuálne otázky trestného práva z pohľadu európskeho a medzinárodného práva
Authors:   Univerzita Pavla Jozefa Šafárika v Košiciach, Marčoková Becková Dominika
Published: June 2023

Zborník príspevkov zo XVI. ročníka študentského sympózia Ústavu medzinárodného práva a európskeho práva sa venuje vybraným aspektom miesta a úlohy trestného práva z pohľadu súčasného európskeho a medzinárodného práva. Študenti a študentky sa vo svojich príspevkoch zaoberajú aktuálnymi témami trestného práva v práve Európskej únie a medzinárodnom práve. Z pohľadu práva Európskej únie je v tomto smere najaktuálnejšou problematika Európskej prokuratúry, orgánu Únie, ktorý je vybavený právomocou vyšetrovať a trestne stíhať trestné činy poškodzujúce finančné záujmy Únie. Tejto problematike je venovaná prvá časť príspevkov, ktoré sa zaoberajú dôvodmi vzniku Európskej prokuratúry, jej štruktúrou a úlohami, problematikou vedenia vyšetrovania Európskej prokuratúry v podmienkach Slovenskej republiky, ako aj vzťahom Európskej prokuratúry s jej najbližších partnerom, Európskym úradom pre boj proti podvodom. Druhá časť príspevkov reflektuje aktuálne otázky a výzvy trestného práva v medzinárodnom práve. Z hľadiska zameranie ide o príspevky, ktoré sa venujú problematike medzinárodného trestného tribunálu pre Ukrajinu, právnym problémom a výzvam spojeným s jeho vytvorením, problematike zločinu agresie pred Medzinárodným trestným súdom, ako i otázkam imunít predstaviteľov štátov pred vnútroštátnymi súdmi.
Spheroidization Heat Treatment Conditions with Data Analysis in Medium Carbon Cr-Mo Steel for Ultra High Strength Cold Heading
Authors: Jo Yong Deok , Lee Hui Ju , Yi Sung , Jang Byoung Lok
Published: June 2023

The degree to which parameters affect the spheroidization heat treatment of steel was calculated by setting the spheroidization heat treatment conditions of Cr-Mo steel and using data analysis such as S/N ratio and ANOVA. After analyzing the transformation temperatures of the steel, Ac1 and Ac3 , using a DSC, the conditions were set accordingly. The surface hardness was measured for the conditions and used as an evaluation index. The correlation was analyzed by comparing the spheroidized volume fraction and the surface hardness, and the Pearson correlation coefficient was -0.88, proving that a correlation existed between the two values. Using S/N ratio and ANOVA, the degree to which each control parameter affects the decrease in the surface hardness was analyzed, qualitatively and quantitatively. For the S/N ratio, priority affecting the surface hardness for each control parameter was analyzed. The 1st heating temperature was found to have a more preferential effect on the surface hardness than the 1st heating time and the 2nd heating temperature. Using ANOVA, the 1st heating temperature was determined to be a very significant factor with the greatest influence, contributing 73.2% to the surface hardness. Intercritical annealing is a suitable spheroidization heat treatment condition, so if the surface hardness of the steel needs to be reduced using Intercritical annealing, the 1st heating temperature and time should be designed as the priority.
Effect of Mg Content and Cooling Rate After Solidification on the Mechanical Properties of T5-treated Al-Si-Mg Alloy
Authors: Son Hyeon-Woo , Koo Tae-Min , Cho Young-Hee , Lee Jung-Moo
Published: June 2023

The effects of Mg content and cooling rate on the T5 treatment of Al-Si-Mg alloys have been investigated using Avrami kinetics and transmission electron microscopy. The alloys were super-heated at 800 oC for 1 hr followed by degassing treatment at 720 oC for 15 min, and poured into a metallic mold preheated to 250 oC. The as-cast alloys were then T5-treated at a temperature of 190 oC. As the Mg content and/or cooling rate increased, the strength of the T5-treated alloys increased while the elongation decreased over the entire aging time. However, some T5 treatment conditions were effective to at enhancinge elongation as well as strength. The alloy with low Mg content showed excellent elongation in the early stage of aging. B, but as the aging time increased, h. However, the elongation decreased rapidly so that there was showed no significant difference from with the alloy with high Mg content in the over-aging stage. This was due to a coarse and agglomerated precipitate structure, which were likely to be formed by the rapid precipitation and agglomeration of the β′ phase. The alloy with a high cooling rate exhibited superior strength with similar elongation over the entire aging time compared to the slowly cooled alloy. The reason for the decrease in strength for in the slowly cooled alloy was attributed to the coarsening of precipitates and suppression of the formation of fine precipitates during cooling.
Microstructural Evolutions and Strengthening Mechanism according to the Aging Temperatures of a High Si Cast Aluminum Alloy
Authors: Shin Won-Sang , Kim Yoon-Jun
Published: June 2023

A356 cast aluminum alloy contains 7 at.% Si and 0.3 at.% Mg, producing an approximately 50% eutectic microstructure. This high Si content and various casting conditions play a significant role in strengthening A356 alloy, by controlling the eutectic morphology and precipitates of other intermetallic compounds. Understanding how Si-related precipitates and clusters are soluble in the α-matrix is necessary to provide high strength and fatigue resistance to A356 alloys. The aging heat-treatment temperature in the A356 alloy most likely promotes the formation of these precipitates and clusters. The A356 samples were differently aged at temperatures of 110 oC and 130 oC for 2 h, and were labeled 110A, and 130A, respectively. 110A was found to have improved mechanical properties, such as high strength and elongation, compared to 130A, which may be attributed to the formation of secondary phases in the α-phase matrix. Scanning and transmission electron microscopy and atom probe tomography analyses demonstrated Ti2Si precipitation and various-sized cluster formations in 110A. In contrast, 130A had fewer clusters than 110A. Therefore, different aging heat-treatment temperatures relate to a change in the behavior of atoms, affecting the mechanical properties.
The influence of multi-stakeholder networks on the uptake of system of rice intensification among smallholder rice farmers in western Kenya
Authors: Ouma Matilda A. , Ouma Luke O. , Ombati Justus M. , Onyango Christopher O.
Published: June 2023

Multi-stakeholder platforms are useful in driving technology uptake by bringing together stakeholders in a value chain for joint agenda-setting and learning to address a common goal. In this paper, we analyze how multi-stakeholder networks unfold in an innovation platform for networking and capacity building to drive the uptake of the system of rice intensification (SRI) in Oluch irrigation scheme in western Kenya. An innovation platform (IP) was launched at Oluch irrigation scheme in Kenya to enhance networking among stakeholders and facilitate capacity building among farmers. We performed a network analysis to understand the patterns of knowledge exchange among stakeholders in the IP. We further explore the association between innovation platform characteristics and the uptake of SRI practices using regression analyses, correlation analyses and chi-square-tests. Our findings reveal that there was a substantial increase in the uptake of SRI that was largely driven by strategic networks. Extensionists and farmer groups emerged as the most important actors while farmer-to-farmer networks are still nascent in promoting SRI than expected but growing. We conclude by describing the describe a theory of change developed and experienced in the IP. While multi-stakeholder networks may be important drivers of technology uptake, there is a need for careful analysis of strategic networks within the IP that are core to achieving IP goals such as uptake of the technology and ensuring sustainability of the IP.
Computational analysis of heat shock protein (HSP) in Citrus X Sinensis
Authors: Nawaz Samia , Rasool Lubna , Rauf Abdur , Rasul Sumaira
Published: June 2023

Heat shock proteins (HSPs) are molecular chaperones and one of the cell’s most important regulatory proteins present in all species. HSPs are a multigene family classified into six families according to their molecular weight range between 8KDa to 110KDa: HSP100, HSP90, HSP70, HSP60, small heat shock proteins (sHSPs), and ubiquitin. Citrus X Sinensis is very economical, and widely grown in the world. It is one of the best sources of nutrition, vitamin C, fiber, thiamine, folate, and antioxidants. It has a variety of health benefits i.e., protects against cell damage, boosts the immune system, heals wounds, etc. For highlighting the aspect of HSPs of citrus in computing protein, this work has great significance. Present studies are based on the identification and characterization of heat shock proteins in Citrus sinuses. In silico research was performed; Arabidopsis thaliana protein sequences of HSP60, HSP70 and HSP90 were used as a reference to identify these molecular chaperones in Citrus sinuses through Blast Phytozome. Furthermore, physiochemical properties, multiple sequence alignment, Multiple Expectation Maximization for Motif Elicitation (MEME) and phylogeny were constructed for 44 sequences of HSP60, 22 sequences of HSP70 and 23 for HSP90. The best expression of HSPs was found in Endoplasmic Reticulum. The findings of the present studies provide a good understanding of the HSP60, HSP70 and HSP90 families' function and expression site in the cell of Citrus sinuses. It also provides the basic knowledge for further investigations of the putative structure of dynamic proteins.
The DNA threat probing of some chromophores using UV/VIS spectroscopy
Authors: Khan Shahab , Rahman Faizan Ur , Zahoor Muhammad , Haq Azmat Ul , Shah Abdul Bari , Rahman Mudassir Ur , Rahman Hummad Ur
Published: June 2023

Chromosphere such as triaryl methane family of dyes used in various industries can affect the body of living organism system upon the interaction with the most biochemical molecule is the DNA. In this research, the binding mode of triaryl methane family of chromophore dyes that is Bromothymol blue, Bromophenol blue, Bromocresol green and Malachite green with double strand DNA in phosphate buffer at pH 6.9 was investigated through UV/VIS spectroscopy. The study shows that Bromothymol blue undergo non covalent interaction with DNA, bromocresol green interact through groove binding and malachite green and bromophenol blue interact through intercalative and partially through electrostatic mode of interaction with DNA. The studies on the binding nature of chemicals/dyes, which are regarded as small molecules to DNA are important and fundamental issues in life sciences. These dyes were found to be more toxic and care should be taken during disposing them off. Proper disposal of these dyes is needed for safe environment and healthy life.
Influence of the SARS-CoV-2 Omicron (B.1.1.529) variant and booster vaccine doses on the seroprevalence of specific IgG antibodies in the staff of the Slovak Academy of Sciences
Authors: Kajanova Ivana , Jelenska Lenka , Lukacikova Lubomira , Grossmannova Katarina , Belisova Martina , Istvanova Nikola , Gasparovicová Natalia , Zelnik Vladimir , Pastorekova Silvia , Radikova Zofia , Kopacek Juraj
Published: June 2023

The presented seroprevalence study focused on specific antibodies to the SARS-CoV-2 virus is the second survey conducted among SAS employees. Its realization enabled monitoring of the impact of booster vaccination doses and the spread of the Omicron variant in a defined group of people. The total seropositivity of the involved SAS employees in autumn 2022 was 96.04%. In the group of vaccinated participants (1,189) the seropositivity rate was 99.5%, while among unvaccinated participants (176) it was 72.73%. By October 2022, when the study was conducted, 65.05% (888) of the participants have had a positive PCR/Ag test for SARS-CoV-2 at least once. Based on the presence of antibodies against the nucleoprotein (NCP) of SARS-CoV-2 it was proven, that 27.39% of participants (25.12% of vaccinated; 51.22% of non-vaccinated) who have never had a positive PCR/Ag test for SARS-CoV-2, overcame the COVID-19. According to self-assessment of the disease course, it was shown that a severe course occurred in 6.31% of the participants who overcame the disease without prior vaccination and in 1.44% of the participants who overcame COVID-19 after completing the baseline vaccination scheme. The most significant finding of the study is the evidence of significantly lower levels of specific antibodies after overcoming the Omicron variant of SARS-CoV-2, and thus its reduced immunogenicity compared to ancestral virus and earlier variants of concern.
Dietary intake of xylose impacts the transcriptome and proteome of tissues involved in xylose metabolism in swine
Authors: Huntley Nichole F. , de Souza Marcela M. , Schulte Matthew D. , Beiki Hamid , de Lima Andressa O. , Jantzi Abigail E. , Lonergan Steven M. , Huff-Lonergan Elisabeth J. , Patience John F. , Koltes James E.
Published: June 2023

Xylose is a primary component of arabinoxylan in swine diets. As arabinoxylan is a significant component of fiber, and fiber is generally rising in practical pig diets globally, the study of arabinoxylan and xylose is of increasing interest. However, the mechanisms by which free xylose may be absorbed and the pathways impacted by xylose have yet to be elucidated in pigs. The objective of this study was to determine the impact of xylose supplementation on gene expression and protein abundance in jejunum, kidney, liver, and muscle tissues which have previously been identified as possible sites of xylose absorption or metabolism. This study aimed to expand the preliminary understanding of dietary xylose metabolism and utilization in pigs. One study, replicated twice with 24 crossbred gilts, was used to assess two dietary treatments: a xylose-free (0%) control and 8% D-xylose. The impact of xylose on growth was monitored by measuring initial and final body weight, serum IGF-1, and liver glycogen concentrations. The rate and efficiency of weight gain were reduced on the xylose diet but not to a level that would occur if xylose was not used at all; the detection of xylose systemically further supports this conclusion. This study confirmed that pigs can utilize dietary xylose. To determine the impact of xylose on tissue metabolism, samples were collected from all four tissues for gene expression analysis by RNA-sequencing, and kidney and liver samples were subjected to proteomic analysis using 2D-DIGE and mass spectrometry. The majority of differentially expressed (DE) genes were identified in the kidney samples (n = 157), with a few identified in the jejunum (n = 16), liver (n = 1), and muscle (n = 20) samples. The DE genes in the kidney were mainly identified as being involved in lipid biosynthesis and fatty acid metabolism. Proteomic results corroborated these findings. Although the inclusion of xylose in a diet at practical levels is shown to impact energy metabolic processes, it has been confirmed that this five-carbon sugar can support levels of growth only slightly below those of glucose, a six-carbon sugar that is more commonly utilized as an energy source in pig diets.
Pullulanase: unleashing the power of enzyme with a promising future in the food industry
Authors: Naik Bindu , Kumar Vijay , Goyal S. K. , Dutt Tripathi Abhishek , Mishra Sadhna , Joakim Saris Per Erik , Kumar Akhilesh , Rizwanuddin Sheikh , Kumar Vivek , Rustagi Sarvesh
Published: June 2023

Pullulanases are the most important industrial group of enzymes in family 13 glycosyl hydrolases. They hydrolyze either α-1,6 and α-1,4 or both glycosidic bonds in pullulan as well as other carbohydrates to produce glucose, maltose, and maltotriose syrups, which have important uses in food and other related sectors. However, very less reports are available on pullulanase production from native strains because of low yield issues. In line with the increasing demands for pullulanase, it has become important to search for novel pullulanase-producing microorganisms with high yields. Moreover, high production costs and low yield are major limitations in the industrial production of pullulanase enzymes. The production cost of pullulanase by using the solid-state fermentation (SSF) process can be minimized by selecting agro-industrial waste. This review summarizes the types, sources, production strategies, and potential applications of pullulanase in different food and other related industries. Researchers should focus on fungal strains producing pullulanase for better yield and low production costs by using agro-waste. It will prove a better enzyme in different food processing industries and will surely reduce the cost of products.