bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenotypic Plasticity: What Has DNA Methylation Got to Do with It?
Authors: Duncan Elizabeth J. , Cunningham Christopher B. , Dearden Peter K.
Published: January 2022

How does one genome give rise to multiple, often markedly different, phenotypes in response to an environmental cue? This phenomenon, known as phenotypic plasticity, is common amongst plants and animals, but arguably the most striking examples are seen in insects. Well-known insect examples include seasonal morphs of butterfly wing patterns, sexual and asexual reproduction in aphids, and queen and worker castes of eusocial insects. Ultimately, we need to understand how phenotypic plasticity works at a mechanistic level; how do environmental signals alter gene expression, and how are changes in gene expression translated into novel morphology, physiology and behaviour? Understanding how plasticity works is of major interest in evolutionary-developmental biology and may have implications for understanding how insects respond to global change. It has been proposed that epigenetic mechanisms, specifically DNA methylation, are the key link between environmental cues and changes in gene expression. Here, we review the available evidence on the function of DNA methylation of insects, the possible role(s) for DNA methylation in phenotypic plasticity and also highlight key outstanding questions in this field as well as new experimental approaches to address these questions.
Sociodemographic Disparities and Parity in Relation to Urinary Incontinence: A Nationwide Primary Healthcare Cohort Study (1997–2018)
Authors: Sundqvist Christoffer , Li Xinjun , Sundquist Kristina , Jansåker Filip
Published: January 2022

Objectives: Urinary incontinence (UI) is a very common condition in the primary healthcare settings. Few studies have investigated whether sociodemographic factors are related to UI. This nationwide study aimed to investigate whether there is a relationship between sociodemographic factors and UI in women. Methods: A nationwide open cohort study included 2,044,065 women aged 15–50 years. Several national population-based (Sweden) databases including nationwide primary healthcare data were used. The outcome was the time to the first event of any UI diagnosis during the study period (1997–2018). Cox regression models were used to test for associations between individual sociodemographic factors and UI. Results: The study identified 44,250 UI events. These corresponded to 2.16% of the study population and an incidence rate (IR) per 1000 person-years of 1.85 (95% CI 1.84–1.87). In the fully adjusted model, a high age, low education level, and being born outside of Sweden were independently associated with a higher UI risk, while rural living was associated with a lower risk. The income level did not seem to have a large impact. Most notably, women born in the Middle East/North Africa and Latin America/Caribbean had a substantially higher risk of UI with HRs of 2.41 (95% CI 2.33–2.49) and 2.30 (95% CI 2.17–2.43), respectively. Parity was strongly and independently associated with UI. Conclusion: This study presents novel risk factors associated with UI. The findings provide new knowledge concerning the burden of this disease among women, which could be used to provide more equal healthcare for these patients in the future. Previous research allied with these findings suggests using a comprehensive approach targeting health disparities.
Digital Online Anaesthesia Patient Informed Consent before Elective Diagnostic Procedures or Surgery: Recent Practice in Children—An Exploratory ESAIC Survey (2021)
Authors: Neumann Claudia , Schleifer Grigorij , Strassberger-Nerschbach Nadine , Kamp Johannes , Massoth Gregor , Görtzen-Patin Alexandra , Cudian Dishalen , Velten Markus , Coburn Mark , Schindler Ehrenfried , Wittmann Maria
Published: January 2022

Background: One undisputed benefit of digital support is the possibility of contact reduction, which has become particularly important in the context of the COVID-19 pandemic. However, to the best of our knowledge, there is currently no study assessing the Europe-wide use of digital online pre-operative patient information or evaluation in the health sector. The aim of this study was to give an overview of the current status in Europe. Methods: A web-based questionnaire covering the informed consent process was sent to members of the European Society of Anaesthesia and Intensive Care Medicine (ESAIC) in 47 European countries (42,433 recipients/930 responses). Six questions related specifically to the practice in paediatrics. Results: A total of 70.2% of the respondents indicated that it was not possible to obtain informed consent via the Internet in a routine setting, and 67.3% expressed that they did not know whether it is in line with the legal regulations. In paediatric anaesthesia, the informed consent of only one parent was reported to be sufficient by 77.6% of the respondents for simple interventions and by 63.8% for complex interventions. Just over 50% of the respondents judged that proof of identity of the parents was necessary, but only 29.9% stated that they ask for it in clinical routine. In the current situation, 77.9% would favour informed consent in person, whereas 60.2% could imagine using online or telephone interviews as an alternative to a face-to-face meeting if regulations were changed. Only 18.7% participants reported a change in the regulations due to the current pandemic situation. Conclusion: Whether informed consent is obtained either online or on the telephone in the paediatric population varies widely across Europe and is not currently implemented as standard practice. For high-risk patients, such as the specific cohort of children with congenital heart defects, wider use of telemedicine might provide a benefit in the future in terms of reduced contact and reduced exposure to health risks through additional hospital stays.
Topological Voting Method for Image Segmentation
Authors: Nguyen Nga T. T. , Le Phuong B.
Published: January 2022

Image segmentation is one of the main problems in image processing. In order to improve the accuracy of segmentation, one often creates a number of masks (annotations) for a same image and then uses some voting methods on these masks to obtain a more accurate mask. In this paper, we propose a voting method whose voting rule is not pixel-wise but takes into account the natural geometric-topological properties of the masks. On three concrete examples, we show that our voting method outperforms the usual arithmetical voting method.
Low-Density Lipoprotein Cholesterol Is a Possible Blood Biomarker of Schizoid Personality Traits among Females
Authors: Hayakawa Kohei , Watabe Motoki , Horikawa Hideki , Sato-Kasai Mina , Shimokawa Norihiro , Nakao Tomohiro , Kato Takahiro A.
Published: January 2022

Lower serum levels of low-density lipoprotein cholesterol (LDL-C) have been suggested to indicate higher suicide risk and various psychiatric symptoms. Previously, we reported that lower serum LDL-C levels are associated with loneliness, social phobia, isolated life with little social support, and lower trust in others among young non-clinical females. Thus, we hypothesize that schizoid personality traits may be associated with lower serum LDL-C. We here verified this hypothesis using non-clinical data and clinical data with schizophrenia. Using the database from the Midlife in Japan (MIDJA), a cohort of residents living in Tokyo, we analyzed whether schizoid-related interpersonal characteristics were associated with LDL-C. In addition, we assessed the association between blood biomarkers including LDL-C and schizoid personality traits in 101 adult non-clinical volunteers. Finally, we evaluated the interaction between LDL-C and social decision making of patients with schizophrenia. In female non-clinical volunteers, serum LDL-C level was a predictive factor and negatively correlated with schizoid personality traits. Female patients with schizophrenia, whose serum LDL-C levels were lower, tended not to trust other females. The present findings suggest that LDL-C may influence schizoid personality traits in females, which provide a basis for further investigation into the biological aspects of schizoid personality disorder.
Land Use Change in the Cross-Boundary Regions of a Metropolitan Area: A Case Study of Tongzhou-Wuqing-Langfang
Authors: Dai Linlin , Zhan Zixin , Shu Yeshuo , Rong Xiao
Published: January 2022

Since the 1980s, metropolitan areas have increased worldwide due to urbanization and regionalization. While the spatial integration of the labor and housing markets has benefitted the development of cities within metropolitan areas, they have also brought great challenges for land governance; this is particularly evident in cross-boundary regions due to the complex relations between the markets and the regulations and between governments at different levels. Extensive research has been conducted on the city-level analysis of socioeconomic integration, land use development, and urban governance within metropolitan areas; yet, it is insufficient for understanding the intricate interplay between the various forces in such regions. This study aims to reveal the dynamics of land use change from 1990–2020 and its driving forces in the recent decade in the Tongzhou-Wuqing-Langfang (TWL) region—a typical cross-boundary area between Beijing, Tianjin, and the Hebei Metropolitan Area—using Landsat imagery. We employed the land-use dynamic degree, kernel density analysis, principal component analysis, and multiple linear regression to explore the spatiotemporal patterns of land use change and its driving factors at the district/county level. The results show that the general land use changes from cultivated and forest land to urban and rural construction land across the region. The speed of the trend varies considerably over time between different areas as the land use policies and regulations of each local government change. The population growth and the tertiary and secondary industry growth are the main driving factors for the change in construction land across the whole TWL region, while the urbanization rate and fixed asset investment have different impacts across the cross-boundary region. The results suggest that expanding the integration of land use policies and regulations in the cross-boundary region is urgently required.
Silver Nanoparticle Production by the Cyanobacterium Cyanothece sp.: De Novo Manipulation of Nano-Biosynthesis by Phytohormones
Authors: Mohamed Maged E. , El Semary Nermin A. , Younis Nancy S.
Published: January 2022

Background: Numerous cyanobacteria have the potential to reduce metallic ions to form pure metal nanoparticles in a green biosynthesis process. Aim: To investigate the production capacity of silver nanoparticles by the cyanobacterium Cyanothece sp. and to examine the effect of five different phytohormones, indole acetic acid, kinetin; gibberellic acid; abscisic acid; and methyl jasmonate, on this capacity. Methods: The cyanobacterial strain was grown for 60 days and the harvested cyanobacterium biomass was incubated with 0.1 mM of AgNO3. Percentage conversion of Ag+ to Ag0 was calculated to indicate the AgNPs’ production capacity. Different concentrations of the five phytohormones were added to cultures and the AgNP production was monitored throughout different time intervals. Results: Cyanothece sp. biosynthesized spherical AgNPs (diameter range 70 to 140 nm, average diameter 84.37 nm). The addition of indole acetic acid and kinetin provoked the maximum conversion (87.29% and 55.16%, respectively) of Ag+ to Ag0, exceeding or slightly below that of the control (56%). Gibberellic and abscisic acids failed to elevate the Ag+ to Ag0 conversion rate (45.23% and 47.95%, respectively) above that of the control. Methyl jasmonate increased the Ag+ to Ag0 conversion rate to 90.29%, although nearly all the cyanobacterial cultures died at the end. Conclusion: Phytohormones could be used to induce or inhibit the green production of AgNPs with the cyanobacterium Cyanothece sp. This novel manipulation technique may have several applications in agriculture or biomedicine.
The Effects of Persistent Olfactory and Gustatory Dysfunctions on Quality of Life in Long-COVID-19 Patients
Authors: Vaira Luigi Angelo , Gessa Claudia , Deiana Giovanna , Salzano Giovanni , Maglitto Fabio , Lechien Jerome R. , Saussez Sven , Piombino Pasquale , Biglio Andrea , Biglioli Federico , Boscolo-Rizzo Paolo , Hopkins Claire , Parma Valentina , De Riu Giacomo
Published: January 2022

(1) Background: Persistent olfactory (POD) and gustatory (PGD) dysfunctions are one of the most frequent symptoms of long-Coronavirus Disease 2019 but their effect on the quality of life (QoL) of patients is still largely unexplored. (2) Methods: An online survey was administered to individuals who reported to have had SARS-CoV-2 infection at least 6 months prior with persisting COVID-19 symptoms (using the COVID symptom index), including ratings of POD and PGD, and their physical (PCS) and mental (MCS) components of quality of life were assessed using the standardized short form 12 questionnaire (SF-12). (3) Results: Responses from 431 unique individuals were included in the analyses. The most frequent persistent symptoms were: fatigue (185 cases, 42.9%), olfactory dysfunction (127 cases, 29.5%), gustatory dysfunction (96 cases, 22.3%) and muscle pain (83 cases, 19.3%). Respondents who reported persisting muscle pain, joint pain, fatigue, headache, gastrointestinal disturbances, and dyspnea had significantly worse PCS. Those experiencing persistent fatigue and dyspnea also showed significantly lower MCS. Respondents reporting POD or PGD showed significantly worse QoL, but only pertaining to the MCS. Multiple regressions predicted MCS based on olfactory and marginally on gustatory ratings, but not PCS. Age significantly affected the prediction of PCS but not MCS, and gender and temporal distance from the COVID-19 diagnosis had no effect. (4) Conclusions: POD and PGD are frequent symptoms of the long-COVID-19 syndrome and significantly reduce QoL, specifically in the mental health component. This evidence should stimulate the establishment of appropriate infrastructure to support individuals with persistent CD, while research on effective therapies scales up.
An Increase in Peripheral Temperature following Cocaine Administration Is Mediated through Activation of Dopamine D2 Receptor in Rats
Authors: Chang Suchan , Ryu Yeonhee , Bang Se Kyun , Jang Han Byeol , Ahn DanBi , Kim Hyung Kyu , Lee Hubert , Kim Sang Chan , Lee Bong Hyo , Kim Hee Young
Published: January 2022

Drug addiction has become a worldwide problem, affecting millions of people across the globe. While the majority of mechanistic studies on drug addiction have been focused on the central nervous system, including the mesolimbic dopamine system, the peripheral actions of drugs of abuse remain largely unknown. Our preliminary study found that the systemic injection of cocaine increased peripheral skin temperature. This led us to our present study, which investigated the mechanisms underlying the increase in peripheral temperature following cocaine injection. Male Sprague Dawley rats were anesthetized with pentobarbital sodium, and peripheral skin temperature measurements were taken using a thermocouple needle microprobe and an infrared thermal camera. Cocaine injection caused an acute rise in peripheral body temperature, but not core body temperature, about 10 min after injection, and the temperature increases were occluded by systemic injection of dopamine D2 receptor antagonist L741,626, but not D1 receptor antagonist SCH23390. In addition, systemic administration of bromocriptine, a dopamine D2 receptor agonist, significantly increased peripheral temperature. Infrared thermal imaging showed that the thermal increases following cocaine injection were predominantly in the distal areas of the forelimbs and hindlimbs, relative to core body temperature. Treatment with cocaine or bromocriptine decreased the size of skin blood vessels without affecting the expression of dopamine D2 receptors. These results suggest that increased peripheral temperature in skin following cocaine injection is associated with the activation of the dopamine D2 receptor.
Analysis of the Physicochemical Properties of Some Selected Non-Edible Vegetable Oil-Based Cutting Fluids Using the Design of Experiment (DOE) Approach
Authors: Kazeem Rasaq A. , Fadare David A. , Ikumapayi Omolayo M. , Akinlabi Stephen A. , Afolalu Sunday A. , Akinlabi Esther T.
Published: January 2022

The pursuit of environmentally friendly cutting fluids is ongoing as the problems of traditional cutting fluids become more apparent. The bulk of vegetable oils used in studies are consumable and, if marketed, may conflict with human utilization, making cutting fluids more costly. However, few investigations on the use of lesser-known vegetable oils as cutting fluids have been conducted. As a result, the goal of this research is to examine how effective some lesser-known vegetable oils will be if used as a cutting fluid. Based on some physicochemical properties such as acid value and viscosity, the developed vegetable oil-based cutting fluids (watermelon and jatropha) were statistically analyzed using 24 full factorial techniques. This paper aimed at determining the interaction of the physicochemical properties on the developed cutting fluids. Emulsifiers, antifoam agents, biocide, and anti-corrosive agents were considered as natural variables. The effect of the natural variables on the acidity index and viscosity of cutting fluids were fully analyzed with ANOVA, contour, and surface plots. From the responses obtained from the experiment as explained by the independent variables (emulsifying agent, anti-corrosion agent, biocide, and antifoam agent), the watermelon seed oil-based cutting fluid was 89% accurate, while the jatropha oil-based cutting fluid was 75% accurate.
Reductive Modification of Carbon Nitride Structure by Metals—The Influence on Structure and Photocatalytic Hydrogen Evolution
Authors: Alwin Emilia , Wojcieszak Robert , Kočí Kamila , Edelmannová Miroslava , Zieliński Michał , Suchora Agata , Pędziński Tomasz , Pietrowski Mariusz
Published: January 2022

Pt, Ru, and Ir were introduced onto the surface of graphitic carbon nitride (g-C3N4) using the wet impregnation method. A reduction of these photocatalysts with hydrogen causes several changes, such as a significant increase in the specific surface area, a C/N atomic ratio, a number of defects in the crystalline structure of g-C3N4, and the contribution of nitrogen bound to the amino and imino groups. According to the X-ray photoelectron spectroscopy results, a transition layer is formed at the g-C3N4/metal nanoparticle interphase, which contains metal at a positive degree of oxidation bonded to nitrogen. These structural changes significantly enhanced the photocatalytic activity in the production of hydrogen through the water-splitting reaction. The activity of the platinum photocatalyst was 24 times greater than that of pristine g-C3N4. Moreover, the enhanced activity was attributed to significantly better separation of photogenerated electron–hole pairs on metal nanoparticles and structural distortions of g-C3N4.
New Graphene Composites for Power Engineering
Authors: Knych Tadeusz , Mamala Andrzej , Kwaśniewski Paweł , Kiesiewicz Grzegorz , Smyrak Beata , Gniełczyk Marek , Kawecki Artur , Korzeń Kinga , Sieja-Smaga Eliza
Published: January 2022

Intensive research is underway worldwide to develop new conductive materials for applications in the power industry. Such tests aim to increase the electrical conductivity of materials for conductors and cables, thus increasing the current carrying capacity of the line and reducing the loss of electricity transmission. The scientific discovery of recent years, graphene, one of the allotropic types of carbon with very high electrical and thermal conductivity and mechanical strength, creates great opportunities for designing and producing new materials with above-standard operational properties. This project concentrates on developing technology for manufacturing aluminum-graphene and copper-graphene composites intended to be used to produce a new generation of power engineering conductors. In particular, we present the results of the research on the mechanical synthesis of aluminum-graphene and copper -graphene composites, as well as the results of the electric, mechanical, and structural properties of rods obtained after the extrusion process and wires after the drawing process.
A Possibility for Quantitative Detection of Mechanically-Induced Invisible Damage by Thermal Property Measurement via Entropy Generation for a Polymer Material
Authors: Sakai Takenobu , Takase Naohiro , Oya Yutaka , Koyanagi Jun
Published: January 2022

Entropy generation from a mechanical and thermal perspective are quantitatively compared via molecular dynamic (MD) simulations and mechanical and thermal experiments. The entropy generation values regarding mechanical tensile loading—which causes invisible damage—of the Polyamide 6 (PA6) material are discussed in this study. The entropy values measured mechanically and thermally in the MD simulation were similar. To verify this consistency, mechanical and thermal experiments for measuring entropy generation were conducted. The experimentally obtained mechanical entropy was slightly less than that calculated by MD simulation. The thermal capacity is estimated based on the specific heat capacity measured by differential scanning calorimetry (DSC), applying the assumed extrapolation methods. The estimated entropy generation was higher than the aforementioned values. There is a possibility that the entropy-estimating method used in this study was inappropriate, resulting in overestimations. In any case, it is verified that entropy increases with mechanical loading and material invisible damage can be qualitatively detected via thermal property measurements.
Dual-Facets Emissive Quantum-Dot Light-Emitting Diode Based on AZO Electrode
Authors: Chen Jing , Huang Qianqian , Lei Wei
Published: January 2022

We report on a green, dual emissive quantum-dot light-emitting diode (QLED) using alumina (Al)-doped ZnO (AZO) to adjust the band offset between the cathode and QD-emitting layers. The dual emissive QLED structure was designed by enhancing the efficient hole injection/transfer and slowing down the electron injection/transfer from AZO to the QD. The QLEDs presented a maximum luminance of 9450 cd/m2, corresponding to a power efficiency of 15.7 lm/W, a current efficiency of 25.5 cd/A, as well as a turn-on voltage of 2.3 V. It is worth noting that the performance of the dual emissive QLED is comparable to that of a single emissive QLED. Therefore, there is a 1.3-fold enhancement in the performance of the QLED based on the AZO cathode due to the balanced charge injection/transfer.
Study of the EDM Process of Bimetallic Materials Using a Composite Electrode Tool
Authors: Ablyaz Timur Rizovich , Shlykov Evgeny Sergeevich , Muratov Karim Ravilevich , Zhurin Alexander Valentinovich
Published: January 2022

New types of profile products make complex use of bimetals. These materials possess a set of properties such as strength, corrosion resistance, thermal conductivity, heat resistance, wear resistance. For the processing of such products, it is advisable to use electrophysical processing methods, one of which is the technology of copy-piercing electrical discharge machining (EDM). Currently, EDM is one of the most common methods for processing products from modern bimetal materials. An urgent task is to study the EDM process of bimetallic materials. The aim of the work was to increase the efficiency and accuracy of the EDM process of bimetallic products using electrode-tools with different physical and mechanical properties. Bimetal—weld coated steel backing, base material—09G2S steel, surfacing material—M1 copper were used. The processing of the bimetallic workpiece was carried out on an Electronica Smart CNC copy-piercing EDM machine. EI used graphite, copper, and composite. A theoretical model was developed that allows calculation of the amount of removal of bimetallic material of steel–copper depending on the EDM modes and the ET (electrode tool) material. During the processing of the steel layer, regardless of the EI material, microcracks were formed along the grain boundaries, and during the processing of the copper layer, enlarged holes resulted.
Soft Chemistry Synthesis and Characterization of CoFe1.8RE0.2O4 (RE3+ = Tb3+, Er3+) Ferrite
Authors: Gingasu Dana , Mindru Ioana , Ianculescu Adelina-Carmen , Diamandescu Lucian , Surdu Vasile-Adrian , Marinescu Gabriela , Bartha Cristina , Preda Silviu , Popa Marcela , Chifiriuc Mariana Carmen
Published: January 2022

Nanosized CoFe1.8RE0.2O4 (RE3+ = Tb3+, Er3+) ferrites were obtained through wet ferritization method. These ferrites were characterized by X-ray diffraction (XRD), scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM/HR-TEM), Fourier transform infrared spectroscopy (FTIR), Mössbauer spectroscopy and magnetic measurements. The XRD results revealed that the average crystallite size is 5.77 nm for CoFe1.8Tb0.2O4 and 6.42 nm for CoFe1.8Er0.2O4. Distribution of metal cations in the spinel structure estimated from X-ray diffraction data showed that the Tb3+ and Er3+ ions occupy the octahedral sites. TEM images indicated the presence of polyhedral particles with average size 5.91 nm for CoFe1.8Tb0.2O4 and 6.80 nm for CoFe1.8Er0.2O4. Room temperature Mössbauer spectra exhibit typical nanoscaled cobalt ferrite spectra in good agreement with XRD and TEM data. The saturation magnetization value (Ms) is 60 emu/g for CoFe1.8Tb0.2O4 and 80 emu/g for CoFe1.8Er0.2O4. CoFe1.8RE0.2O4 nanoparticles showed similar antimicrobial efficacy against the five tested microbial strains, both in planktonic and biofilm state. The results highlight the promising potential of these types of nanoparticles for the development of novel anti-biofilm agents and materials.
Nitrate Removal by Donnan Dialysis and Anion-Exchange Membrane Bioreactor Using Upcycled End-of-Life Reverse Osmosis Membranes
Authors: Lejarazu-Larrañaga Amaia , Ortiz Juan M. , Molina Serena , Pawlowski Sylwin , Galinha Claudia F. , Otero Vanessa , García-Calvo Eloy , Velizarov Svetlozar , Crespo João G.
Published: January 2022

This work explores the application of Reverse Osmosis (RO) upcycled membranes, as Anion Exchange Membranes (AEMs) in Donnan Dialysis (DD) and related processes, such as the Ion Exchange Membrane Bioreactor (IEMB), for the removal of nitrate from contaminated water, to meet drinking water standards. Such upcycled membranes might be manufactured at a lower price than commercial AEMs, while their utilization reinforces the commitment to a circular economy transition. In an effort to gain a better understanding of such AEMs, confocal µ-Raman spectroscopy was employed, to assess the distribution of the ion-exchange sites through the thickness of the prepared membranes, and 2D fluorescence spectroscopy, to evaluate alterations in the membranes caused by fouling and chemical cleaning The best performing membrane reached a 56% average nitrate removal within 24 h in the DD and IEMB systems, with the latter furthermore allowing for simultaneous elimination of the pollutant by biological denitrification, thus avoiding its discharge into the environment. Overall, this work validates the technical feasibility of using RO upcycled AEMs in DD and IEMB processes for nitrate removal. This membrane recycling concept might also find applications for the removal and/or recovery of other target negatively charged species.
The Evaluation of Counter Diffusion CVD Silica Membrane Formation Process by In Situ Analysis of Diffusion Carrier Gas
Authors: Ishii Katsunori , Nomura Mikihiro
Published: January 2022

A new evaluation method for preparing silica membranes by counter diffusion chemical vapor deposition (CVD) was proposed. This is the first attempt to provide new insights, such as the decomposition products, membrane selectivity, and precursor reactivity. The permeation of the carrier gas used for supplying a silica precursor was quantified during the deposition reaction by using a mass spectrometer. Membrane formation processes were evaluated by the decrease of the permeation of the carrier gas derived from pore blocking of the silica deposition. The membrane formation processes were compared for each deposition condition and precursor, and the apparent silica deposition rates from the precursors such as tetramethoxysilane (TMOS), hexyltrimethoxysilane (HTMOS), or tetraethoxysilane (TEOS) were investigated by changing the deposition temperature at 400–600 °C. The apparent deposition rates increased with the deposition temperature. The apparent activation energies of the carrier gas through the TMOS, HTMOS, and TEOS derived membranes were 44.3, 49.4, and 71.0 kJ mol−1, respectively. The deposition reaction of the CVD silica membrane depends on the alkoxy group of the silica precursors.
Modified Poly(vinylidene fluoride) by Diethylenetriamine as a Supported Anion Exchange Membrane for Lithium Salt Concentration by Hybrid Capacitive Deionization
Authors: Siekierka Anna , Bryjak Marek
Published: January 2022

This paper shows the investigation for the optimal anion exchange membranes (AEM) supporting the desorption step of the HCDI process. The chemical modification of PVDF by diethylene triamine created the AEM. To confirm the ion-exchange character of materials, the chemical analysis with FTIR, SEM, surface energetics, and transportation analysis were applied. Next, the investigated membranes were applied for the sorption and desorption of lithium chloride. The specific sorptive parameters were higher according to the incorporation of the nitrogen groups into polymeric chains. Considering the desorption efficiency, membranes modified by four days were selected for further evaluation. The application in the HCDI process allowed reaching the desorption efficiency at 90%. The system composed of PVDF-DETA4 membrane was suitable for sorption 30 mg/g of salt. By applying the PVDF-DETA4 membrane, it is possible to concentrate LiCl with four factors. The anion exchange character of the developed membrane was confirmed by adsorption kinetics and isotherms of chlorides, nitrates, sodium, and lithium. The prepared membrane could be considered a perspective material suitable for concentration salt with electro-driven technologies for the above reasons.
Data Driven Performance Prediction in Steel Making
Authors: Boto Fernando , Murua Maialen , Gutierrez Teresa , Casado Sara , Carrillo Ana , Arteaga Asier
Published: January 2022

This work presents three data-driven models based on process data, to estimate different indicators related to process performance in a steel production process. The generated models allow the optimization of the process parameters to achieve optimal performance and quality levels. A new approach based on ensembles has been developed with feature selection methods and four state-of-the-art regression approximations (random forest, gradient boosting, xgboost and neural networks). The results show that the proposed approach makes the prediction more stable reducing the variance for all cases, even in one case, slightly reducing the bias. Furthermore, from the four machine learning paradigms presented, random forest is the one with the best results in a quantitative way, obtaining a coefficient of determination of 0.98 as a maximum, depending on the target sub-process.
Effect of Nickel Contents on Fatigue Crack Growth Rate and Fracture Toughness for Nickel Alloy Steels
Authors: Park Jeong Yeol , Kim Byoung Koo , Nam Dae Geun , Kim Myung Hyun
Published: January 2022

In terms of steel alloying elements, generally, nickel is used as an austenite stabilizing element to increase the toughness of steel. The low temperature materials, such as nickel alloy steels with a nickel content of 3.5% to 9%, stainless steel and Invar, show excellent toughness at low (173 K) and cryogenic (108 K) temperatures. In particular, in the shipbuilding industry, it is mainly used for liquefied ethane and Liquefied Natural Gas (LNG) carriers, and research on low-temperature steels are attracting attention again as regulations on environmental issues are strengthened in recent years. Therefore, in this study, fatigue and fracture performances of nickel alloy steel containing 9% or less among nickel alloy steels are evaluated. Moreover, we assess the Fatigue Ductile to Brittle Transition (FDBT) of nickel alloy steels based on crack tip opening displacement (CTOD). In order to discuss the fatigue and fracture performances of nickel alloy steels, microstructure analysis carried out. As a result, CTOD and Fatigue Crack Growth Rate (FCGR) of nickel alloy steels increases as nickel contents increase. In addition, FDBT of 9% nickel alloy steel is the lowest compared to other nickel alloy steels.
Phylogenetic, Functional and Safety Features of 1950s B. infantis Strains
Authors: Duboux Stéphane , Ngom-Bru Catherine , De Bruyn Florac , Bogicevic Biljana
Published: January 2022

Strains of Bifidobacterium longum subsp. infantis (B. infantis) are amongst the first to colonize the infant gut, partly due to their capacity to metabolize complex human milk oligosaccharides (HMO), and are proposed to play a key role in the development of the infant gut. Since early life, B. infantis supplementation is of high interest, and detailed phylogenetic, functional and safety characterization of the selected strains should be pursued. Using a combination of long and short-read sequencing technologies, we first decipher the genetic distance between different isolates of the same B. infantis strain. Using the same approach, we show that several publicly available genomes recapitulate this strain-level distance as compared to two of the first strains obtained in the 1950s. Furthermore, we demonstrate that the two 1950s B. infantis strains display different functional and safety attributes, as ATCC 15697 is resistant to streptomycin and shows a preference towards lacto-N-tetraose LNT and sialylated HMOs, while LMG 11588 is sensitive to all tested antibiotics and shows a preference towards fucosylated HMOs. Overall, our work highlights that the current diversity observed in B. infantis is likely underestimated and that strain selection within this subspecies must be the subject of scientific pursuit and associated evaluation.
Microplastics (Polystyrene) Exposure Induces Metabolic Changes in the Liver of Rare Minnow (Gobiocypris rarus)
Authors: Wang Chunling , Hou Miaomiao , Shang Kunyu , Wang Huanshan , Wang Jianwei
Published: January 2022

Microplastics are environmental contaminants and an emergent concern. Microplastics are abundant in freshwater and can cause biochemical stress in freshwater organisms. In the current study, rare minnows (Gobiocypris rarus) were exposed to 1μm polystyrene microplastics at 200 μg/L concentration. We observed various sublethal effects after four weeks of exposure but no mortality. Numerous cellular and tissue alterations were observed in the liver. Differential metabolites and differentially expressed genes between control and exposure groups were identified and mapped to pathways in the Kyoto Encyclopedia of Genes and Genomes. The combination of transcriptomic and metabolomic analyses revealed significantly varied metabolic pathways between the two groups. These pathways were involved in glucolipid, amino acid, and nucleotide metabolism. Results demonstrated that MP exposure induced immune reaction, oxidative stress, and disturbed glycolipid and energy metabolism. The current study provided novel insights into the molecular and metabolic mechanisms of microplastic ecotoxicology in rare minnow.
Comparison Studies on Several Ligands Used in Determination of Cd(II) in Rice by Flame Atomic Absorption Spectrometry after Ultrasound-Assisted Dispersive Liquid–Liquid Microextraction
Authors: Sun Qian , Cui Xinyu , Wang Yanfeng , Zhang Pingping , Lu Wenjuan
Published: January 2022

Ligands plays an important role in the extraction procedures for the determination of cadmium in rice samples by using flame atomic absorption spectrometry (FAAS). In the present study, comparative evaluation of 10 commercially available ligands for formation of Cd(II)-ligand complex and determination of cadmium in rice samples by ultrasound-assisted dispersive liquid–liquid microextraction (UADLLME) combined with FAAS was developed. Sodium diethyldithiocarbamate (DDTC) provided a high distribution coefficient as well as a good absorbance signal, therefore DDTC was used as a ligand in UADLLME. A low density and less toxic solvent, 1-heptanol, was used as the extraction solvent and ethanol was used as the disperser solvent. In addition, the experimental conditions of UADLLME were optimized in standard solution first and then applied in rice, such as the type and volume of extractant and dispersant, pH, extraction time, and temperature. Under the optimal experimental conditions, the detection limit (3σ) was 0.69 μg/L for Cd(II). The proposed method was applied for the determination of Cd(II) in three different rice samples (polished rice, brown rice, and glutinous rice), the recovery test was carried out, and the results ranged between 96.7 to 113.6%. The proposed method has the advantages of simplicity, low cost, and accurate and was successfully applied to analyze Cd(II) in rice.
Prognostic Value of Immunohistochemical Markers for Locally Advanced Rectal Cancer
Authors: Taha Anas , Taha-Mehlitz Stephanie , Petzold Stephanie , Achinovich Sergey L. , Zinovkin Dmitry , Enodien Bassey , Pranjol Md Zahidul I. , Nadyrov Eldar A.
Published: January 2022

The aim of this study is to reveal the potential roles of apoptosis markers (Bcl2 and p53), proliferation markers (Ki-67 and CyclD1), and the neuroendocrine marker Chromogranin A as markers for the radioresistance of rectal cancer. Statistically significant differences were found in the expression of p53, Ki-67, and Chromogranin A in groups of patients with and without a favorable prognosis after radiotherapy. The survival analysis revealed that the marker of neuroendocrine differentiation, Chromogranin A, also demonstrated a high prognostic significance, indicating a poor prognosis. Markers of proliferation and apoptosis had no prognostic value for patients who received preoperative radiotherapy. Higher Chromogranin A values were predictors of poor prognosis. The results obtained from studying the Chromogranin A expression suggest that the secretion of biologically active substances by neuroendocrine cells causes an increase in tumor aggressiveness.
Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots
Authors: Giordano Ady , Morales-Tapia Pablo , Moncada-Basualto Mauricio , Pozo-Martínez Josué , Olea-Azar Claudio , Nesic Aleksandra , Cabrera-Barjas Gustavo
Published: January 2022

Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
Prion Strains Differ in Susceptibility to Photodynamic Oxidation
Authors: Kostelanska Marie , Holada Karel
Published: January 2022

Prion disorders, or transmissible spongiform encephalophaties (TSE), are fatal neurodegenerative diseases affecting mammals. Prion-infectious particles comprise of misfolded pathological prion proteins (PrPTSE). Different TSEs are associated with distinct PrPTSE folds called prion strains. The high resistance of prions to conventional sterilization increases the risk of prion transmission in medical, veterinary and food industry practices. Recently, we have demonstrated the ability of disulfonated hydroxyaluminum phthalocyanine to photodynamically inactivate mouse RML prions by generated singlet oxygen. Herein, we studied the efficiency of three phthalocyanine derivatives in photodynamic treatment of seven mouse adapted prion strains originating from sheep, human, and cow species. We report the different susceptibilities of the strains to photodynamic oxidative elimination of PrPTSE epitopes: RML, A139, Fu-1 > mBSE, mvCJD > ME7, 22L. The efficiency of the phthalocyanine derivatives in the epitope elimination also differed (AlPcOH(SO3)2 > ZnPc(SO3)1-3 > SiPc(OH)2(SO3)1-3) and was not correlated to the yields of generated singlet oxygen. Our data suggest that the structural properties of both the phthalocyanine and the PrPTSE strain may affect the effectiveness of the photodynamic prion inactivation. Our finding provides a new option for the discrimination of prion strains and highlights the necessity of utilizing range of prion strains when validating the photodynamic prion decontamination procedures.
Preparation of Deuterium Labeled Compounds by Pd/C-Al-D2O Facilitated Selective H-D Exchange Reactions
Authors: Kokel Anne , Kadish Dora , Török Béla
Published: January 2022

The chemo/regioselective H-D exchange of amino acids and synthetic building blocks by an environmentally benign Pd/C-Al-D2O catalytic system is described. Due to the importance of isotope labeled compounds in medicinal chemistry and structural biology, notably their use as improved drug candidates and biological probes, the efficient and selective deuteration methods are of great interest. The approach is based on selective H-D exchange reactions where the deuterium source is simple D2O. D2 gas is generated in situ from the reaction of aluminum and D2O, while the commercially available palladium catalyst assists the H-D exchange reaction. The high selectivity and efficiency, as well as the simplicity and safe nature of the procedure make this method an environmentally benign alternative to current alternatives.
Multidrug Resistance (MDR): A Widespread Phenomenon in Pharmacological Therapies
Authors: Catalano Alessia , Iacopetta Domenico , Ceramella Jessica , Scumaci Domenica , Giuzio Federica , Saturnino Carmela , Aquaro Stefano , Rosano Camillo , Sinicropi Maria Stefania
Published: January 2022

Multidrug resistance is a leading concern in public health. It describes a complex phenotype whose predominant feature is resistance to a wide range of structurally unrelated cytotoxic compounds, many of which are anticancer agents. Multidrug resistance may be also related to antimicrobial drugs, and is known to be one of the most serious global public health threats of this century. Indeed, this phenomenon has increased both mortality and morbidity as a consequence of treatment failures and its incidence in healthcare costs. The large amounts of antibiotics used in human therapies, as well as for farm animals and even for fishes in aquaculture, resulted in the selection of pathogenic bacteria resistant to multiple drugs. It is not negligible that the ongoing COVID-19 pandemic may further contribute to antimicrobial resistance. In this paper, multidrug resistance and antimicrobial resistance are underlined, focusing on the therapeutic options to overcome these obstacles in drug treatments. Lastly, some recent studies on nanodrug delivery systems have been reviewed since they may represent a significant approach for overcoming resistance.
Analytical Evaluation of Carbamate and Organophosphate Pesticides in Human and Environmental Matrices: A Review
Authors: Mdeni Nonkululeko Landy , Adeniji Abiodun Olagoke , Okoh Anthony Ifeanyi , Okoh Omobola Oluranti
Published: January 2022

Pesticides are synthetic compounds that may become environmental contaminants through their use and application. The high productivity achieved in the agricultural industry can be credited to the use and application of pesticides by way of pest and insect control. As much as pesticides have a positive impact on the agricultural industry, some disadvantages come with their application in the environment because they are intentionally toxic, and this is more towards non-target organisms. They are grouped into chlorophenols, organochlorines, synthetic pyrethroid, carbamates, and organophosphorus based on their structure. The symptoms of exposure to carbamate (CM) and organophosphates (OP) are similar, although poisoning from CM is of a shorter duration. The analytical evaluation of carbamate and organophosphate pesticides in human and environmental matrices are reviewed using suitable extraction and analytical methods.
Inverse Identification of Single-Crystal Plasticity Parameters of HCP Zinc from Nanoindentation Curves and Residual Topographies
Authors: Nguyen Pham T. N. , Abbès Fazilay , Lecomte Jean-Sébastien , Schuman Christophe , Abbès Boussad
Published: January 2022

This paper investigates the orientation-dependent characteristics of pure zinc under localized loading using nanoindentation experiments and crystal plasticity finite element (CPFEM) simulations. Nanoindentation experiments on different grain orientations exhibited distinct load–depth responses. Atomic force microscopy revealed two-fold unsymmetrical material pile-up patterns. Obtaining crystal plasticity model parameters usually requires time-consuming micromechanical tests. Inverse analysis using experimental and simulated loading–unloading nanoindentation curves of individual grains is commonly used, however the solution to the inverse identification problem is not necessarily unique. In this study, an approach is presented allowing the identification of CPFEM constitutive parameters from nanoindentation curves and residual topographies. The proposed approach combines the response surface methodology together with a genetic algorithm to determine an optimal set of parameters. The CPFEM simulations corroborate with measured nanoindentation curves and residual profiles and reveal the evolution of deformation activity underneath the indenter.