bluuebunny/crossref_metadata_2025 huggingface dataset:
An empirical study of supporting executive function in family education with mental abacus
Authors: Watanabe Nobuki
Published: May 2023

In the field of childcare, the focus has primarily been on executive function (EF) (working memory [WM]), which is a critical factor in daily life. The Wechsler Intelligence Scale for Children’s (WISC-IV) WM Index subtests of the digit span (forward and backward) and letter–number sequencing tasks are used to assess EF (WM). Theoretically, the letter–number sequencing task is more demanding on EF (WM) than the digit span task. Additionally, there is some suggestion of a relationship between EF (WM) and mental abacus (MA) and that MA can easily be performed in daily life. One challenge of supporting EF (WM) is that it is hard to conduct general evaluations and daily activities at home. This study examines the following questions through an empirical study: (1) Can Mental Abacus be used to support executive function in family education? In this study, I measured the cerebral blood flow of one child at home using the HOT-2000 for the tasks of MA and the WISC-IV. As a result, I was able to show that for that child, MA could have an effect on EF(WM). Although this is only one example, for parents, it has been important in showing that it is not impossible in the field of home education where there are many different kinds of children. The findings provide a new perspective regarding evaluating EF (WM) in cognitive psychology and neuropsychology and are valuable in breaking through previous research.
Aversive effects of volatiles from intact plants help to fine tune host-searching behavior of the parasitoid wasp, Cotesia kariyai
Authors: Ross Socheat , Matsuyama Shigeru , Furukawa Seiichi , Kuramitsu Kazumu , Kainoh Yooichi
Published: May 2023

Herbivore-induced plant volatiles (HIPVs) from infested plants are exploited by parasitoid wasps as cues to search for host habitat. However, uninfested (intact) and infested plants are often intermingled in nature, and how volatiles released by intact plants affect host-searching behavior in natural habitats remains unknown. In a previous study, using a wind tunnel, we found that intact maize plants negatively influenced landing behavior of a parasitoid wasp, Cotesia kariyai (Hymenoptera: Braconidae), when they were placed downwind of infested plants. To determine whether intact plant volatiles affect host searching by C. kariyai, we collected volatiles from intact plants using a headspace volatile collection technique. In a wind tunnel, wasps landed less frequently on a green paper model treated with intact plant volatiles placed in front of an infested plant, than on a green paper model treated with the solvent, hexane. In a 4-arm olfactometer, wasps spent significantly less time in an arena containing mixed volatiles from intact and infested plants, compared to the same arena treated only with volatiles from infested plants. Both results indicate that intact plant volatiles re-direct host-searching behavior in C. kariyai females. Chemical analyses suggest differences in volatiles between intact and infested plants. In terms of parasitoid adaptations for effective searching for hosts in complex environments, aversive effects of intact plant volatiles may help C. kariyai to locate patches of infested plants.
Refined expression quantitative trait locus analysis on adenocarcinoma at the gastroesophageal junction reveals susceptibility and prognostic markers
Authors: Zhong Ce , Wu Chen , Lin Yuan , Lin Dongxin
Published: May 2023

Objectives: This study aimed to explore cell type level expression quantitative trait loci (eQTL) in adenocarcinoma at the gastroesophageal junction (ACGEJ) and identify susceptibility and prognosis markers.

Methods: Whole-genome sequencing (WGS) was performed on 120 paired samples from Chinese ACGEJ patients. Germline mutations were detected by GATK tools. RNA sequencing (RNA-seq) data on ACGEJ samples were taken from our previous studies. Public single-cell RNA sequencing (scRNA-seq) data were used to produce the proportion of epithelial cells. Matrix eQTL and a linear mixed model were used to identify condition-specific cis-eQTLs. The R package coloc was used to perform co-localization analysis with the public data of genome-wide association studies (GWASs). Log-rank and Cox regression tests were used to identify survival-associated eQTL and genes. Functions of candidate risk loci were explored by experimental validation.

Results: Refined eQTL analyses of paired ACGEJ samples were performed and 2,036 potential ACGEJ-specific eQTLs with East Asian specificity were identified in total. ACGEJ-gain eQTLs were enriched at promoter regions more than ACGEJ-loss eQTLs. rs658524 was identified as the top eQTL close to the transcription start site of its paired gene (CTSW). rs2240191–RASAL1, rs4236599–FOXP2, rs4947311–PSORS1C1, rs13134812–LOC391674, and rs17508585–CDK13-DT were identified as ACGEJ-specific susceptibility eQTLs. rs309483–LINC01355 was associated with the overall survival of ACGEJ patients. We explored functions of candidate eQTLs such as rs658524, rs309483, rs2240191, and rs4947311 by experimental validation.

Conclusion: This study provides new risk loci for ACGEJ susceptibility and effective disease prognosis biomarkers.
The impact of microbially modified metabolites associated with obesity and bariatric surgery on antitumor immunity
Authors: Wang Meng , Huang Yuhong , Xin Meiling , Li Tianxing , Wang Xueke , Fang Yini , Liang Shufei , Cai Tianqi , Xu Xiaoxue , Dong Ling , Wang Chao , Xu Zhengbao , Song Xinhua , Li Jingda , Zheng Yanfei , Sun Wenlong , Li Lingru
Published: May 2023

Obesity is strongly associated with the occurrence and development of many types of cancers. Patients with obesity and cancer present with features of a disordered gut microbiota and metabolism, which may inhibit the physiological immune response to tumors and possibly damage immune cells in the tumor microenvironment. In recent years, bariatric surgery has become increasingly common and is recognized as an effective strategy for long-term weight loss; furthermore, bariatric surgery can induce favorable changes in the gut microbiota. Some studies have found that microbial metabolites, such as short-chain fatty acids (SCFAs), inosine bile acids and spermidine, play an important role in anticancer immunity. In this review, we describe the changes in microbial metabolites initiated by bariatric surgery and discuss the effects of these metabolites on anticancer immunity. This review attempts to clarify the relationship between alterations in microbial metabolites due to bariatric surgery and the effectiveness of cancer treatment. Furthermore, this review seeks to provide strategies for the development of microbial metabolites mimicking the benefits of bariatric surgery with the aim of improving therapeutic outcomes in cancer patients who have not received bariatric surgery.
Mycobacterium tuberculosis cell-wall and antimicrobial peptides: a mission impossible?
Authors: Jacobo-Delgado Yolanda M. , Rodríguez-Carlos Adrian , Serrano Carmen J. , Rivas-Santiago Bruno
Published: May 2023

Mycobacterium tuberculosis (Mtb) is one of the most important infectious agents worldwide and causes more than 1.5 million deaths annually. To make matters worse, the drug resistance among Mtb strains has risen substantially in the last few decades. Nowadays, it is not uncommon to find patients infected with Mtb strains that are virtually resistant to all antibiotics, which has led to the urgent search for new molecules and therapies. Over previous decades, several studies have demonstrated the efficiency of antimicrobial peptides to eliminate even multidrug-resistant bacteria, making them outstanding candidates to counterattack this growing health problem. Nevertheless, the complexity of the Mtb cell wall makes us wonder whether antimicrobial peptides can effectively kill this persistent Mycobacterium. In the present review, we explore the complexity of the Mtb cell wall and analyze the effectiveness of antimicrobial peptides to eliminate the bacilli.
Effects of transgenerational photoperiod experience on the reproduction and development of Anastatus orientalis, an egg parasitoid of the spotted lanternfly
Authors: Bao Ke-xin , Wang Xiao-yi , Cao Liang-ming , Xin Bei , Broadley Hannah J. , Gould Juli R.
Published: May 2023

Transgenerational experience can affect a range of natural enemies’ life-history traits and can be involved in the control of developmental plasticity. As a major egg parasitoid of the spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), the wasp Anastatus orientalis (Hymenoptera: Eupelmidae) is effective at suppressing its host populations. The reproductive and developmental traits of A. orientalis is known to depend on photoperiod conditions, but transgenerational photoperiodic effects have yet to be evaluated. To evaluate the transgenerational photoperiodic effects on A. orientalis, we assessed wasp adult longevity, female fecundity, sex ratio, and diapause rate over three consecutive generations under different experimental photoperiods (L16:D8, L12:D12, and L8:D16), using Antheraea pernyi (Lepidoptera: Saturniidae) eggs as hosts. The results suggest that transgenerational experience significantly impacts several biological parameters of progeny. All parasitoids entered a diapause under the long photoperiod condition (i.e., L16:D8), after which the number of female parasitoids and fecundity of the 2nd and 3rd generations increased significantly as compared to the 1st generation. With the long photoperiod conditions, the female ratio rose from 68.1% (1st generation) to 86.0% (3rd generation) and the progeny per females increased from 35.8 to 75.7. However, adult longevity of females and males were shortened significantly. With the intermediate photoperiod (L12:D12) conditions, fecundity and sex ratio of the 2nd and 3rd generations increased significantly as compared to the 1st generation. With the short photoperiod (L8:D16) conditions, there were no significant differences in fecundity among three generations, but sex ratio of the 2nd and 3rd generations increased significantly as compared to the 1st generation. These results on transgenerational photoperiodic effects can be applied to improve laboratory rearing efficiency of parasitoids and to better understand population dynamics in the field across a latitudinal gradient.
The importance of chest CT severity score and lung CT patterns in risk assessment in COVID-19-associated pneumonia: a comparative study
Authors: Szabó Miklós , Kardos Zsófia , Kostyál László , Tamáska Péter , Oláh Csaba , Csánky Eszter , Szekanecz Zoltán
Published: May 2023

Introduction

Chest computed tomography (CT) is suitable to assess morphological changes in the lungs. Chest CT scoring systems (CCTS) have been developed and use in order to quantify the severity of pulmonary involvement in COVID-19. CCTS has also been correlated with clinical outcomes. Here we wished to use a validated, relatively simple CTSS to assess chest CT patterns and to correlate CTSS with clinical outcomes in COVID-19.

Patients and methods

Altogether 227 COVID-19 cases underwent chest CT scanning using a 128 multi-detector CT scanner (SOMATOM Go Top, Siemens Healthineers, Germany). Specific pathological features, such as ground-glass opacity (GGO), crazy-paving pattern, consolidation, fibrosis, subpleural lines, pleural effusion, lymphadenopathy and pulmonary embolism were evaluated. CTSS developed by Pan et al. (CTSS-Pan) was applied. CTSS and specific pathologies were correlated with demographic, clinical and laboratory data, A-DROP scores, as well as outcome measures. We compared CTSS-Pan to two other CT scoring systems.

Results

The mean CTSS-Pan in the 227 COVID-19 patients was 14.6 ± 6.7. The need for ICU admission (p < 0.001) and death (p < 0.001) were significantly associated with higher CTSS. With respect to chest CT patterns, crazy-paving pattern was significantly associated with ICU admission. Subpleural lines exerted significant inverse associations with ICU admission and ventilation. Lymphadenopathy was associated with all three outcome parameters. Pulmonary embolism led to ICU admission. In the ROC analysis, CTSS>18.5 significantly predicted admission to ICU (p = 0.026) and CTSS>19.5 was the cutoff for increased mortality (p < 0.001). CTSS-Pan and the two other CTSS systems exerted similar performance. With respect to clinical outcomes, CTSS-Pan might have the best performance.

Conclusion

CTSS may be suitable to assess severity and prognosis of COVID-19-associated pneumonia. CTSS and specific chest CT patterns may predict the need for ventilation, as well as mortality in COVID-19. This can help the physician to guide treatment strategies in COVID-19, as well as other pulmonary infections.
Functional limitation as a mediator of the relationship between multimorbidity on health-related quality of life in Australia: evidence from a national panel mediation analysis
Authors: Lee John Tayu , Ishida Marie , Haregu Tilahun , Pati Sanghamitra , Zhao Yang , Palladino Raffaele , Anindya Kanya , Atun Rifat , Oldenburg Brian , Marthias Tiara
Published: May 2023

Objective

The inverse relationships between chronic disease multimorbidity and health-related quality of life (HRQoL) have been well-documented in the literature. However, the mechanism underlying this relationship remains largely unknown. This is the first study to look into the potential role of functional limitation as a mediator in the relationship between multimorbidity and HRQoL.

Methods

This study utilized three recent waves of nationally representative longitudinal Household, Income, and Labor Dynamics in Australia (HILDA) surveys from 2009 to 2017 (n = 6,814). A panel mediation analysis was performed to assess the role of functional limitation as a mediator in the relationship between multimorbidity and HRQoL. The natural direct effect (NDE), indirect effect (NIE), marginal total effect (MTE), and percentage mediated were used to calculate the levels of the mediation effect.

Results

This study found that functional limitation is a significant mediator in the relationship between multimorbidity and HRQoL. In the logistic regression analysis, the negative impact of multimorbidity on HRQoL was reduced after functional limitation was included in the regression model. In the panel mediation analysis, our results suggested that functional limitation mediated ~27.2% (p < 0.05) of the link between multimorbidity and the composite SF-36 score for HRQoL. Functional limitation also mediated the relationship between the number of chronic conditions and HRQoL for each of the eight SF-36 dimensions, with a proportion mediated ranging from 18.4 to 28.8% (p < 0.05).

Conclusion

Functional status has a significant impact on HRQoL in multimorbid patients. Treatment should concentrate on interventions that improve patients' functioning and mitigate the negative effects of multimorbidity.
Supplementation of Lactobacillus plantarum ATCC14917 mitigates non-alcoholic fatty liver disease in high-fat-diet-fed rats
Authors: Wen Xingjian , Liu Hejing , Luo Xiaoling , Lui Li , Fan Jiuyu , Xing Yajing , Wang Jia , Qiao Xingfang , Li Na , Wang Guixue
Published: May 2023

Atherosclerosis and non-alcoholic fatty liver disease (NAFLD) have been increasing at an alarming rate worldwide. Many clinical studies have underlined the link between NAFLD and atherosclerosis. Our previous experiments have discovered that Lactobacillus (L.) plantarum ATCC14917 supplementation could decrease the progression of atherosclerotic lesion formation. In this study, we aimed to investigate the role of supplementation of L. plantarum ATCC14917 mitigates liver injury in rats fed with a high-fat diet (HFD, 45% kcal from fat). A total of 32 rats were randomly divided into four groups, including two intervention groups, who fed with HFD and administering either 1 × 107 or 1 × 109 colony forming units (CFU) of L. plantarum ATCC14917, the normal control group, and the HFD control group. The results showed that supplementation with low-dose and high-dose of L. plantarum ATCC14917 for 8 weeks could alleviate the body weight gain (p < 0.05), hepatic steatosis, and serum lipid metabolism (p < 0.05) in HFD-fed rats. Moreover, supplementation of L. plantarum ATCC 14917 decreased total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels (p < 0.05) in serum, and improved HFD-associated inflammation (p < 0.05). Furthermore, cecal contents were analyzed by high-throughput 16S ribosomal RNA sequencing. The results indicated that supplementation of L. plantarum ATCC 14917 could ameliorate HFD-induced gut dysbiosis. In summary, our findings suggest that supplementation of L. plantarum ATCC 14917 could mitigate NAFLD in rats, suggesting it may be considered as a probiotic agent for preventing HFD-induced obesity.
Aniline-induced production of aniline-containing polyketides and related bicyclic polyketides by the Yellow River wetland-derived fungus Talaromyces funiculosus
Authors: Wang Zhenhui , Li Zhanlin , Niu Chao , Yang Lanping , Zhai Yangyang , Li Dehai , Wu Guangwei , Zhang Zhenzhen , He Xueqian
Published: May 2023

Introduction and Methods

Silencing gene activation can effectively enrich the diversity of fungal secondary metabolites.

Results and Discussion

Cultivation of the Yellow River wetland-derived fungus Talaromyces funiculosus HPU-Y01 with aniline led to the isolation of one new aniline-containing polyketide tanicutone A (1), two new bicyclic polyketides tanicutones B-C (2–3), a new related trienoic acid 8-methyldeca-2,4,6-trienoic acid (5), and a known compound 4. The planar structures and configurations of 1–5 were determined by NMR, MS, and ECD calculations. Compound 2 featured a key aldehyde group and showed promising inhibitory activity against Vibrio parahaemolyticus with a minimum inhibitory concentration (MIC) value of 0.17 μg/mL. This is a rare report of aniline-induced fungal production of tetrahydronaphthone polyketides.
Classification of gait phases based on a machine learning approach using muscle synergy
Authors: Park Heesu , Han Sungmin , Sung Joohwan , Hwang Soree , Youn Inchan , Kim Seung-Jong
Published: May 2023

The accurate detection of the gait phase is crucial for monitoring and diagnosing neurological and musculoskeletal disorders and for the precise control of lower limb assistive devices. In studying locomotion mode identification and rehabilitation of neurological disorders, the concept of modular organization, which involves the co-activation of muscle groups to generate various motor behaviors, has proven to be useful. This study aimed to investigate whether muscle synergy features could provide a more accurate and robust classification of gait events compared to traditional features such as time-domain and wavelet features. For this purpose, eight healthy individuals participated in this study, and wireless electromyography sensors were attached to four muscles in each lower extremity to measure electromyography (EMG) signals during walking. EMG signals were segmented and labeled as 2-class (stance and swing) and 3-class (weight acceptance, single limb support, and limb advancement) gait phases. Non-negative matrix factorization (NNMF) was used to identify specific muscle groups that contribute to gait and to provide an analysis of the functional organization of the movement system. Gait phases were classified using four different machine learning algorithms: decision tree (DT), k-nearest neighbors (KNN), support vector machine (SVM), and neural network (NN). The results showed that the muscle synergy features had a better classification accuracy than the other EMG features. This finding supported the hypothesis that muscle synergy enables accurate gait phase classification. Overall, the study presents a novel approach to gait analysis and highlights the potential of muscle synergy as a tool for gait phase detection.
Central nervous system tumors in patients coming from areas of conflict in the Middle East/North Africa region: an experience from King Hussein Cancer Center
Authors: Obeidat Mouness , Nazzal Jamil , Al Sharie Sarah , Al-Azzam Ahmed Mahmoud , Maswadeh Ahmad , Al-Abdallat Haneen , Ismail Layan , Alkderat Marah , Hzayen Ro’ya , Al-Sheble Yasmeen , Mansour Asem , Al-Hussaini Maysa
Published: May 2023

Introduction

The global cancer burden has been disproportionately shifting towards low- and middle-income countries (LMICs). Limited availability and accessibility to screening, treatment and surveillance, increase in the prevalence and lack of control of risk factors, and underdeveloped healthcare infrastructures have greatly contributed to the disparity in the global cancer burden.

Methods

A retrospective cohort study was conducted that included adult and pediatric patients with an established diagnosis of Central Nervous System (CNS) tumors including brain or spinal tumors of which different demographic, clinical characteristics, and financial burden were presented.

Results

749 patients were included stemming from various countries in the Middle East/North Africa (MENA) region including Libya (34.2%), Palestine (19.8%), Iraq (15.4%), Syria (14.6%) Yemen (14.5%), and Sudan (1.5%). Most patients were adults (66%) with a median age of 34-year-old. 104 patients had died (13.9%), 80 patients were still alive (10.7%) and most of the patients (n= 565, 75.5%) were lost to follow-up. The added cost of managing these patients is 10,172,935 Jordanian Dinars (JOD), with King Hussein Cancer Foundation (KHCF) covering around 34.3% of the total cost.

Conclusion

Our study aimed at taking a closer look at patients coming from areas of conflict in the MENA region diagnosed and treated for CNS tumors at King Hussein Cancer Center (KHCC) over a 12-year period. It was found that even with the contributions of the Jordanian sources almost half of the patients were faced with the entire financial burden of treatment alone.
Transcriptomic signature defines two subtypes of locally advanced PCa with distinct neoadjuvant therapy benefits
Authors: Zhu Yinjie , Fan Liancheng , Zhu Hanjing , Gong Yiming , Chi Chenfei , Wang Yanqing , Pan Jiahua , Dong Baijun , Xue Wei
Published: May 2023

Background

Patients with locally advanced prostate cancer (LAPCa) received docetaxel-based neoadjuvant chemo-hormonal therapy (NCHT) had better clinical outcomes after surgery compared to neoadjuvant hormonal therapy (NHT) groups, but not all patients experienced favorable clinical outcomes with NCHT, raising the necessity for potential biomarker assessment. The transcriptomic profiling offers a unique opportunity to interrogate the accurate response to NCHT and NHT treatment and to identify the predictive biomarkers for neoadjuvant therapy.

Methods

The whole transcriptomic profiling was performed on baseline biopsies and surgical tissue specimens from 64 patients with LAPCa at Renji Hospital between 2014 and 2018. Biochemical progression-free survival (bPFS)-based gene-by-treatment interaction effects were used to identify predictive biomarkers for guiding treatment selection.

Results

Comparing the transcriptome profiling of pre- and post-treatment LAPCa specimens, NHT and NCHT shared 1917 up- and 670 down-regulated DEGs at least 2-fold. Pathway enrichment analysis showed up-regulated pathways in response to NHT and NCHT were both enriched in cytokine receptor interaction pathways, and down-regulated pathways in response to NCHT were enriched in cell cycle pathways. By comprehensive transcriptome profiling of 64 baseline specimens, ten predictive markers were identified. We integrated them into the signature to evaluate the relative benefits of neoadjuvant therapy, which categorizes patients into two subgroups with relative bPFS benefits from either NHCT or NHT. In the high-score (≥ -95.798) group (n = 37), NCHT treatment led to significantly longer bPFS (P< 0.0001), with a clear and early separation of the Kaplan–Meier curves. In the low-score (< -95.798) group (n = 27), NHT also led to significantly longer bPFS (P=0.0025).

Conclusions

In this study, we proposed the first predictive transcriptomic signature might potentially guide the effective selection of neoadjuvant therapy in LAPCa and might provide precise guidance toward future personalized adjuvant therapy.

Trial registration

The study was approved by the Ethics Committee of Renji Hospital affiliated to Shanghai Jiao Tong University (Ky2019-087).
Medication use in Italian nursing homes: preliminary results from the national monitoring system
Authors: Zito S. , Poluzzi E. , Pierantozzi A. , Onder G. , Da Cas R. , Ippoliti I. , Lunghi C. , Cangini A. , Trotta F.
Published: May 2023

Background: The aging population has increased concerns about the affordability, quality, and nature of long-term care for older people, emphasizing the role of nursing homes. Unlike acute hospital and primary care, there is a lack of drug consumption data in long-term care to understand regional or national healthcare policies.

Objectives: This study aimed to describe medication consumption by older adults and expenditure in Italian nursing homes (NHs).

Methods: Data on drug consumption and costs from the administrative medicine informational flows that detect medicines packages supplied to patients in health facilities and NHs were used. Data on the characteristics of the healthcare residence were from the Italian Health Ministry. Records for the year 2019, selecting the nursing homes exclusively providing elderly or mixed (elderly and disabled) were used.

Results: In 2019, the total expenditure on medicines in NHs amounted to 25.38 million euros, the average cost to 1.30 and the expenditure per bed to 436.18 euros. Cardiovascular drugs were the highest-consuming therapeutic class (177.0 defined daily doses—DDDs/100 days of NH stay; 22.2% of total) followed by drugs acting on the alimentary tract and metabolism (167.6% and 21.0%) and blood drugs (160.4% and 20.1%). The treatment of hypertension and heart failure was widely the most frequently used, with the consumption being driven mainly by furosemide and ramipril. Antiulcer drugs were used on average in more than half of the days of NH stay (58.5 DDDs/100 days of NH stay), representing a therapeutic category for which deprescribing initiatives are recommended. On average, almost all patients received a dose of benzodiazepines, antipsychotics and antidepressants (37.6, 35.9, and 17.7 DDDs/100 days of NH stay, respectively), confirming the high prevalence of use for these medicines. Antibiotics reached 6.8 DDDs/100 days of NH stay.

Conclusion: The availability of data in this specific setting allows the identification of the main interventions toward improving appropriateness and represents a challenge for drug utilization research. Data from this study suggest that proton pump inhibitors (PPIs), benzodiazepines and antibacterials can be areas of improving prescribing appropriateness.
Integrated machine learning and bioinformatic analyses used to construct a copper-induced cell death-related classifier for prognosis and immunotherapeutic response of hepatocellular carcinoma patients
Authors: Zhao Shuai , Chen Shuxian , Liu Wangrui , Wei Shiyin , Wu Xinrui , Cui Dan , Jiang Lifeng , Chen Siyu , Wang Jian
Published: May 2023

Background: Copper as phytonutrient has powerful activity against health diseases. A newly discovered mechanism of cell death that affects energy metabolism by copper (“cuproptosis”) can induce multiple cuproptosis-related genes. Hepatocellular carcinoma (HCC) is a poorly prognosed widespread cancer having danger of advanced metastasis. Therefore, earlier diagnosis followed by the specific targeted therapy are required for improved prognosis. The work herein constructed scoring system built on ten cuproptosis-related genes (CRGs) to predict progression of tumor and metastasis more accurately and test patient reaction toward immunotherapy.

Methods: A comprehensive assessment of cuproptosis patterns in HCC samples from two databases and a real-world cohort was performed on ten CRGs, that were linked to immune cell infiltration signatures of TME (tumor microenvironment). Risk signatures were created for quantifying effect of cuproptosis on HCC, and the effects of related genes on cellular function of HCC were investigated, in addition to the effects of immunotherapy and targeted therapy drugs.

Results: Two distinct cuproptosis-associated mutational patterns were identified, with distinct immune cell infiltration characteristics and survival likelihood. Studies have shown that assessment of cuproptosis-induced tumor mutational patterns can help predict tumor stage, phenotype, stromal activity, genetic diversity, and patient prognosis. High risk scores are characterized by lower survival and worse treatment with anti-PD-L1/CTAL4 immunotherapy and first-line targeted drugs. Cytological functional assays show that CDKN2A and GLS promote proliferation, migration and inhibit copper-dependent death of HCC cells.

Conclusion: HCC patients with high-risk scores exhibit significant treatment disadvantage and survival rates. Cuproptosis plays a non-negligible role in the development of HCC. Quantifying cuproptosis-related designs of tumors will aid in phenotypic categorization, leading to efficient personalized and targeted therapeutics and precise prediction of prognosis and metastasis.
The mechanism of vitamin D3 in preventing colorectal cancer through network pharmacology
Authors: Rong Kang , He Qingmin , Chen Shu , Yu Yong , Mei Lu , Mi Yang , Mu Liufan , Zhu Mingyang , Nan Mengjiao , Zhang Xiaoyang , Wan Zhaoyang , Huang Huang , Zheng Pengyuan
Published: May 2023

Objective: Colorectal cancer (CRC) is a common cancer that cannot be detected at an early stage and is a major challenge in oncology research. Studies have shown that vitamin D3 has some anti-cancer and preventive effects on colorectal cancer, but the exact anti-cancer mechanism is not clear. We applied the relevant research methods of network pharmacology to speculate and validate the possible potential pharmacological mechanisms of vitamin D3 for the prevention of colorectal cancer, and to provide more theoretical support for the clinical anticancer effects of vitamin D3.

Methods: The relevant targets for vitamin D3 and CRC were obtained from the database of drug and disease targets, respectively. The target of vitamin D3 and the target of colorectal cancer were taken to intersect to obtain common targets. Then, the PPI network was constructed. In addition, the pathways of drug-disease interactions were predicted by GO and KEGG enrichment analysis. Finally, the obtained results were verified to ensure the reliability of the experiments.

Results: 51 targets of vitamin D3 for the prevention of colorectal cancer were obtained. The 10 core targets were obtained from the PPI network. The 10 core targets include: ALB, SRC, MMP9, PPARG, HSP90AA1, IGF1, EGFR, MAPK1, MAP2K1 and IGF1R. The core targets were further validated by molecular docking and animal experiments. The results suggest that vitamin D3 plays a key role in the prevention of CRC through core targets, PI3K-Akt pathway, HIF-1 pathway, and FoxO pathway.

Conclusion: This study will provide more theoretical support for vitamin D3 to reduce the incidence of CRC and is important to explore more pharmacological effects of vitamin D3.
Genome-wide identification of NF-Y gene family in maize (Zea mays L.) and the positive role of ZmNF-YC12 in drought resistance and recovery ability
Authors: Cao Liru , Ma Chenchen , Ye Feiyu , Pang Yunyun , Wang Guorui , Fahim Abbas Muhammad , Lu Xiaomin
Published: May 2023

Nuclear factor Y (NF-Y) genes play important roles in many biological processes, such as leaf growth, nitrogen nutrition, and drought resistance. However, the biological functions of these transcription factor family members have not been systematically analyzed in maize. In the present study, a total of 52ZmNF-Ygenes were identified and classified into three groups in the maize genome. An analysis of the evolutionary relationship, gene structure, and conserved motifs of these genes supports the evolutionary conservation of NF-Y family genes in maize. The tissue expression profiles based on RNA-seq data showed that all genes apart fromZmNF-Y16,ZmNF-YC15, andZmNF-YC17were expressed in different maize tissues. A weighted correlation network analysis was conducted and a gene co expression network method was used to analyze the transcriptome sequencing results; six core genes responding to drought and rewatering were identified. A real time fluorescence quantitative analysis showed that these six genes responded to high temperature, drought, high salt, and abscisic acid (ABA) treatments, and subsequent restoration to normal levels.ZmNF-YC12was highly induced by drought and rewatering treatments. The ZmNF-YC12 protein was localized in the nucleus, and the Gal4-LexA/UAS system and a transactivation analysis demonstrated thatZmNF-YC12in maize (Zea mays L.) is a transcriptional activator that regulates drought resistance and recovery ability. SilencingZmNF-YC12reduced net photosynthesis, chlorophyll content, antioxidant (superoxide dismutase, catalase, peroxidase and ascorbate peroxidase) system activation, and soluble protein and proline contents; it increased the malondialdehyde content, the relative water content, and the water loss rate, which weakened drought resistance and the recoverability of maize. These results provide insights into understanding the evolution of ZmNF-Y family genes in maize and their potential roles in genetic improvement. Our work provides a foundation for subsequent functional studies of the NF-Y gene family and provides deep insights into the role of theZmNF-YC12regulatory network in controlling drought resistance and the recoverability of maize.
Comparison of the volatile organic compounds in Citrus reticulata ‘Chachi’ peel with different drying methods using E-nose, GC-IMS and HS-SPME-GC-MS
Authors: Wang Min , Li Xue , Ding Haiyan , Chen Hongping , Liu Youping , Wang Fu , Chen Lin
Published: May 2023

Introduction

Citrus reticulata ‘Chachi’ peel (CRCP), which is named “Guangchenpi” in China, is a geographical indication product with unique flavor properties. CRCP has been used for centuries as a traditional genuine herb because of its excellent therapeutic effects. In addition, owing to its unique odor and high nutrition, it is widely used in various food preparations. Volatile organic compounds (VOCs) are regarded as an important quality marker for CRCP and are highly susceptible to effects in the drying process due to their thermal instability.

Methods

In the current study, the main VOCs in CRCP were processed using different drying methods, including sun-drying, hot air drying, and vacuum-freeze drying. The VOCs were identified by the electronic nose (E-nose), gas chromatography-ion mobility spectrometry (GC-IMS), and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS).

Results

The results showed that the CRCP dried by vacuum-freeze exhibited the highest VOCs contents and retained the richest compounds compared to those dried by other methods, which indicated that vacuum-freeze drying is the most suitable for CRCP production. Furthermore, the chemometrics analysis revealed that the primary differential metabolites of the samples generated using different drying methods were terpenes and esters.

Discussion

Overall, our study would help better understand the VOCs present in CRCP with different drying methods. The outcomes of the current study would guide the drying and processing of CRCP, which is beneficial for large-scale storage and industrial production of CRCP.
The quality of working life: gap between perception and idealization impact of gender and status
Authors: Salès-Wuillemin Edith , Minondo-Kaghad Brigitte , Chappé Julien , Gélin Morgan , Dolard Adrien
Published: May 2023

Background

Many studies show the existence of gender inequalities at work. For example, in France, only 37% of women have a managerial role, which is far from parity. Among these gender inequalities, the present study considers the Quality of Working Life (QWL) for women and managers.

Method

This study measures the Quality of Working Life (QWL) perceived by individuals according to their gender (Women vs. Men) and their status (Managers vs. Co-workers). A questionnaire was distributed to 1,321 employees. It comprised two scales: the WRQoL scale and the QUALTRA-Scale. The QUALTRA-Scale permits the calculation of an index δ that measures the gap between the ideal QWL and the perceived QWL.

Results

The ANOVA (2×2) revealed an impact of status on the perceived QWL on both scales. There was no gender effect. However, there was an effect of both gender and status on the index δ of the QUALTRA-Scale. In particular, for the Women group, δ was higher for the Social Relationships at Work whereas for the Co-workers, it was higher for the Work Environment.

Conclusion

These results are discussed, highlighting the value of measuring the ideal QWL as a reference point for assessing the QWL.
Resting state connectivity in people living with HIV before and after stopping heavy drinking
Authors: Gullett Joseph M. , DeFelice Jason , Richards Veronica L. , Porges Eric C. , Cohen Ronald A. , Govind Varan , Salan Teddy , Wang Yan , Zhou Zhi , Cook Robert L.
Published: May 2023

Background

Heavy alcohol use in people living with HIV (PLWH) has widespread negative effects on neural functioning. It remains unclear whether experimentally-induced reduction in alcohol use could reverse these effects. We sought to determine the effects of 30-days drinking cessation/reduction on resting state functional connectivity in people with and without HIV.

Methods

Thirty-five participants (48.6% PLWH) demonstrating heavy alcohol use attempted to stop drinking for 30 days via contingency management (CM). MRI was acquired at baseline and after thirty days, and functional connectivity across five resting-state fMRI (rsfMRI) networks was calculated with the Conn toolbox for Matlab and examined in relation to transdermal alcohol concentration (TAC) recorded by the ankle-worn secure continuous remote alcohol monitor (SCRAM) and self-reported alcohol use (timeline follow-back; TLFB). Associations between alcohol use and reduction, HIV status, functional connectivity, and change in functional connectivity across five major rsfMRI networks were determined relative to the pre- and post-CM timepoints.

Results

Baseline resting-state functional connectivity was not significantly associated with average TAC-AUC during the pre-CM period, though higher self-reported alcohol use over the preceding 30 days was significantly associated with higher baseline connectivity within the Dorsal Attention Network (DAN; p-FDR < 0.05). Baseline connectivity within the Salience network was significantly negatively related to objective drinking reduction after intervention (DAN; p-FDR < 0.05), whereas baseline connectivity within the Limbic network was positively associated with self-reported drinking reduction (p-FDR < 0.05). Change in between-networks functional connectivity after intervention was significantly positively associated with biosensor-confirmed drinking reduction such that higher reduction was associated with stronger connectivity between the limbic and fronto-parietal control networks (p-FDR < 0.05). PLWH with lower DAN connectivity at baseline demonstrated poorer alcohol reduction than those with higher DAN connectivity at baseline.

Discussion

Lower resting-state functional connectivity of the Salience network significantly predicted stronger drinking reduction across all participants, suggesting a potential biomarker for reduced susceptibility to the environmental and social cues that often make alcohol use reduction attempts unsuccessful. Increased between-networks connectivity was observed in participants with higher alcohol reduction after CM, suggesting a positive benefit to brain connectivity associated with reduced drinking. PLWH with lower baseline DAN connectivity may not benefit as greatly from CM for alcohol reduction.
Can post stroke walking improve via telerehabilitation? A systematic review in adults with stroke
Authors: Deshmukh Shravni , Madhavan Sangeetha
Published: May 2023

Objectives

The purpose of this systematic review is to analyze primary studies investigating the effects of telerehabilitation on walking outcomes for the treatment of adult stroke survivors.

Methods

Data sources included PubMed, Embase and CINAHL searched until August 2022, using combinations of several keywords such as “telerehabilitation”, “stroke”, and “gait”. Studies were required to have bidirectional form of videoconferencing with assessor presence, and include assessment of walking function (speed, endurance and/or balance). Data extraction was performed from each full text by one author, and quality and bias were assessed using the Physiotherapy Evidence Database (PEDro).

Results

Eight studies involving 248 participants met the inclusion criteria. Seven reported significant improvements in outcomes of balance and two showed improvements in endurance after telerehabilitation. Two studies observed greater balance improvements in the telerehabilitation group compared to control and/or in-person therapy. Differences in frequency, training duration, intervention type, and absence of an in-person therapy control group were identified as causes of variation between studies.

Conclusions

The effectiveness of telerehabilitation as a mode of therapy for walking could not be definitively determined due to the limited number of studies that directly measured walking speed or endurance. However, strong evidence was found for the use of telerehabilitation for balance improvements, which has implications for walking recovery.

Impact statement

Telerehabilitation appears to be safe, feasible and demonstrated high adherence. Our results highlighted limited studies using real-time supervision to administer telerehabilitation and lack of studies focusing on outcomes of walking speed and endurance, needed to fully determine the role of telerehabilitation for gait recovery.

Systematic review registration number

PROSPERO number CRD42021238197.
Towards a circular built environment – Focus on the new M.Sc. program in real estate and facility management
Authors: Wrase Isabelle , Haase Matthias , Wang-Speiser Zifei
Published: May 2023

Introduction

The sustainability transformation of the built environment is one of the mega tasks of the twenty first century. Real estate professionals who manage sustainability transformation must be acknowledged in all disciplines that reside around the lifecycle of a property. In addition, these managers must have expert knowledge of the megatrend of sustainability and possess the highest level of interpersonal skills to enable society and organizations to manage the associated change.

Methods

Considering the current challenges and the critical role of education to empower real estate (RE) and facility management (FM) students to explore new paths of sustainable development and grow into active citizens, conscious producers, and consumers, this contribution investigates new and elective ways of teaching sustainability concepts, specifically circular economy (CE). To this end, 122 European university degree programs were examined based on a review of all course titles to determine whether the relevant keywords and content for enabling future real estate managers to drive the sustainability transformation toward a circular built environment are present.

Results

The results show that topics on sustainability are covered significantly. Graduates are empowered in several disciplines that evolve around the real estate lifecycle, but not in all the required ones. There seem to be gaps in teaching especially in the subject area of CE.

Discussion

The extent to which students are also socially empowered is hard to trace. Furthermore, this article describes and critically discusses how the introduction of a holistic approach to the management of real estate for master-level students could help them gain a sustainability perspective and prepare them to act circularly in the future.
Humans need only 200 ms to generate posture-specific muscle activation patterns for successful vertical jumps in reaction to an auditory trigger
Authors: Bobbert Maarten F. , Koopman Axel S.
Published: May 2023

Introduction

It is currently unknown how the central nervous system controls ballistic whole-body movements like vertical jumps. Here we set out to study the time frame of generating muscle activation patterns for maximum-effort jumps from different initial postures.

Methods

We had ten healthy male participants make a slow countermovement from an upright position and initiate a maximal vertical jump as soon as possible following an auditory trigger. The trigger was produced when hip height dropped below one of three preselected values, unknown in advance to the participant, so that the participant was uncertain about the posture from which to initiate the jump. Furthermore, we determined the ensuing bottom postures reached during jumps, and from these postures had the participants perform maximum-effort squat jumps in two conditions: whenever they felt ready, or as soon as possible following an auditory trigger. Kinematics and ground reaction forces were measured, and electromyograms were collected from gluteus maximus, biceps femoris, rectus femoris, vastus lateralis, gastrocnemius and soleus. For each muscle, we detected activation onsets, as well as reaction times defined as the delay between trigger onset and activation onset.

Results

In the jumps preceded by a slow countermovement, the posture from which to initiate the jump was unknown before trigger onset. Nevertheless, in these jumps, posture-specific muscle activation patterns were already released within 200 ms after trigger onset and reaction times were not longer and jump heights not less than in squat jumps from corresponding bottom postures.

Discussion

Our findings suggest that the generation of muscle activation patterns for jumping does not start before trigger onset and requires only about 200 ms.
Erythema multiforme in a Central American tapir (Tapirus bairdii) calf, clinical case report
Authors: Hernández Wolters Luana , Striedinger Cardona Alejandro
Published: May 2023

A 7-month-old male Central American tapir with a history of recurrent respiratory diseases and repeated prolonged administration of amoxicillin with clavulanic acid presented with lethargy, severe hyperalgesia, and interscapular ulcerating vesicular lesions with serosanguineous content, as well as dorsal skin peeling, oral ulcers, and thoracic limb, abdominal, and submandibular pustules with surrounding erythema and alopecia. The histopathological analysis and clinical manifestations were compatible with erythema multiforme, which was treated with daily wound cleaning and silver sulfadiazine cream application, as well as oral prednisolone for 15 days, with five daily tapering doses, achieving clinical improvement and an adequate cure of the disease. Vesicular dermatitis syndrome of tapirs is a disease complex including erythema multiforme, a condition rarely diagnosed, since the analyses required for a definitive diagnosis are almost never performed; therefore, it is important to carry out the necessary tests that allow the differentiation of the dermatopathies included in this complex of vesicular skin diseases.