bluuebunny/crossref_metadata_2025 huggingface dataset:
The experiences of familial mental illness stigma among individuals living with mental illnesses
Authors: Adu Joseph , Oudshoorn Abram , Anderson Kelly , Marshall Carrie Anne , Stuart Heather
Published: April 2024

Persons with mental illnesses may experience stigma from their immediate family members in addition to other forms of stigma. Using semi-structured interviews, we investigated experiences of familial mental illness stigma among 15 people diagnosed with mental illnesses in a mid-sized city in Canada. We identified five themes that speak to participants’ experiences of familial mental illness stigma and ways to reduce it. The themes include the following: diagnosis as a ‘double-edged sword,’ potential familial isolation, familial stigma as societal stigma localized, stories of acceptance, and confronting potential familial mental illness stigma. Participants’ narratives indicate that familial mental illness stigma is rooted in the broader social or public stigma, which sees its way into familial relations as well. This stigma takes various forms, including relationship bias or unfair treatment, breakdown in romantic relationships, loss of status, verbal and emotional abuse, exclusion from decision-making, and alienation within their immediate and extended families. Familial mental illness stigma experiences negatively impact participant’s psychological well-being and personal empowerment. However, participants also shared ways that family members create supportive environments or actively confront or prevent stigma. Overall, this study has contributed to knowledge on mental illness stigma, particularly familial mental illness stigma from the perspective of participants living with a mental illness in a high-income country. Suggestions for future research include a focus on strategies to prevent ongoing familial mental illness stigma and large-scale studies to explore familial mental illness stigma to understand why families might perpetrate stigma.
Value-based payment for high-cost treatments in Singapore: a qualitative study of stakeholders’ perspectives
Authors: Bayani Diana Beatriz , Wee Hwee Lin
Published: April 2024

Objectives

The rising costs of drugs have necessitated the exploration of innovative payment methods in healthcare systems. Risk-sharing agreements (RSAs) have been implemented in many countries as a value-based payment mechanism to manage the uncertainty associated with expensive technologies. This study aimed to investigate stakeholder perspectives on value-based payment in the Singaporean context, providing insights for future directions in health technology assessment and financing.

Methods

This descriptive qualitative inquiry involved participant interviews conducted between October 2021 and April 2022. Thematic analysis was conducted in two phases to analyze the interview transcripts.

Results

Seventeen respondents participated in the study, and five key themes emerged from the analysis. Stakeholders viewed RSAs as moderately positive, despite limited experience with them. They emphasized the importance of clearly defining objectives and establishing transparent criteria for implementing these schemes. The current data infrastructure was identified as both a barrier and facilitator, as RSAs impose administrative burdens. To successfully implement these payment mechanisms, capacity building, and effective stakeholder engagement that fosters mutual trust and cocreation are crucial.

Conclusion

This study confirms previously identified barriers and facilitators to successful RSA implementation while contextualizing them within the Singaporean setting. The findings suggest that value-based payment has the potential to address uncertainty and improve access to healthcare technologies, but these barriers must be addressed for the schemes to be effective.
High-risk pregnancy and its relationship with the neurodevelopment and behavior of 2-year-old children
Authors: Mariño-Narvaez Carolina , Puertas-Gonzalez Jose A. , Romero-Gonzalez Borja , Cruz-Martinez Milagros , Gonzalez-Perez Raquel , Juncosa-Castro Yaima , Peralta-Ramirez Maria Isabel
Published: April 2024

High-risk pregnancies elevate maternal stress, impacting offspring neurodevelopment and behavior. This study, involving 112 participants, aimed to compare perceived stress, neurodevelopment, and behavior in high-risk and low-risk pregnancies. Two groups, high-risk and low-risk, were assessed during pregnancy for stress using hair cortisol and psychological analysis. At 24 months post-birth, their children’s neurodevelopment and behavior were evaluated. Results revealed higher perceived stress and pregnancy-related concerns in high-risk pregnancies, contrasting with low-risk pregnancies. Offspring from high-risk pregnancies displayed elevated internalizing behavior scores, while low-risk pregnancies showed higher externalizing behavior scores. Additionally, women in low-risk pregnancies exhibited increased cortisol concentrations 24 months post-delivery. These findings underscore the necessity for early stress detection and prevention programs during pregnancy, particularly in high-risk cases, to enhance maternal and infant health.
The future of childhood maltreatment research: Diversity and equity-informed perspectives for inclusive methodology and social justice
Authors: Narayan Angela J. , Brown Michelle P. , Lawler Jamie M.
Published: April 2024

A long-standing practice in clinical and developmental psychology research on childhood maltreatment has been to consider prospective, official court records to be the gold standard measure of childhood maltreatment and to give less weight to adults’ retrospective self-reports of childhood maltreatment, sometimes even treating this data source as invalid. We argue that both formats of assessment – prospective and retrospective – provide important information on childhood maltreatment. Prospective data drawn from court records should not necessarily be considered the superior format, especially considering evidence of structural racism in child welfare. Part I overviews current maltreatment definitions in the context of the developmental psychopathology (DP) framework that has guided maltreatment research for over 40 years. Part II describes the ongoing debate about the disproportionalities of minoritized children at multiple decision-making stages of the child welfare system and the role that racism plays in many minoritized families’ experience of this system. Part III offers alternative interpretations for the lack of concordance between prospective, official records of childhood maltreatment and retrospective self-reports, and for the differential associations between each format of data with health outcomes. Moving forward, we recommend that future DP research on childhood maltreatment apply more inclusive, diversity and equity-informed approaches when assessing and interpreting the effects of childhood maltreatment on lifespan and intergenerational outcomes. We encourage future generations of DP scholars to use assessment methods that affirm the lived experiences of individuals and families who have directly experienced maltreatment and the child welfare system.
IN THE BEGINNING: THE GENESIS AND EARLY YEARS OF THE HES BULLETIN
Authors: Vaughn Karen
Published: April 2024

In 1978, the Executive Committee of the History of Economics Society voted to begin publishing the HES Bulletin to serve as a regular means of communication among members. It was intended to include short notes, research inquiries, and reports about events important to Society members. Karen Vaughn of George Mason University was its first editor, a position she held until 1983. During those years, the content grew too include, in addition to notes and research inquiries, reports on the annual meeting, abstracts of papers presented, and publication of the annual Presidential and Distinguished Scholar Addresses. In 1983, the editorship passed on the William Thweat who expanded the content to include more scholarly papers, leading the way to the eventual establishment of the Journal of the History of Economic Thought.
Modeling the Winter Heat Conduction Through the Sea Ice System During MOSAiC
Authors: Zampieri Lorenzo , Clemens‐Sewall David , Sledd Anne , Hutter Nils , Holland Marika
Published: April 2024

Models struggle to accurately simulate observed sea ice thickness changes, which could be partially due to inadequate representation of thermodynamic processes. We analyzed co‐located winter observations of the Arctic sea ice from the Multidisciplinary Drifting Observatory for the Study of the Arctic Climate for evaluating and improving thermodynamic processes in sea ice models, aiming to enable more accurate predictions of the warming climate system. We model the sea ice and snow heat conduction for observed transects forced by realistic boundary conditions to understand the impact of the non‐resolved meter‐scale snow and sea ice thickness heterogeneity on horizontal heat conduction. Neglecting horizontal processes causes underestimating the conductive heat flux of 10% or more. Furthermore, comparing model results to independent temperature observations reveals a ∼5 K surface temperature overestimation over ice thinner than 1 m, attributed to shortcomings in parameterizing surface turbulent and radiative fluxes rather than the conduction. Assessing the model deficiencies and parameterizing these unresolved processes is required for improved sea ice representation.
Ultra‐Low Velocity Zones Beneath the Southern Hemisphere Imaged With Double‐Array Stacking of PcP Waveforms
Authors: Agboola Kayode J. , Hansen Samantha E. , Garnero Edward J. , Rost Sebastian , Li Mingming , Shim Sang‐Heon
Published: April 2024

Ultra‐low velocity zones (ULVZs) are anomalous structures, generally associated with decreased seismic velocity and sometimes an increase in density, that have been detected in some locations atop the Earth's core‐mantle boundary (CMB). A wide range of ULVZ characteristics have been reported by previous studies, leading to many questions regarding their origins. The lowermost mantle beneath Antarctica and surrounding areas is not located near currently active regions of mantle upwelling or downwelling, making it a unique environment in which to study the sources of ULVZs; however, seismic sampling of this portion of the CMB has been sparse. Here, we examine core‐reflected PcP waveforms recorded by seismic stations across Antarctica using a double‐array stacking technique to further elucidate ULVZ structure beneath the southern hemisphere. Our results show widespread, variable ULVZs, some of which can be robustly modeled with 1‐D synthetics; however, others are more complex, which may reflect 2‐D or 3‐D ULVZ structure and/or ULVZs with internal velocity variability. Our findings are consistent with the concept that ULVZs can be largely explained by variable accumulations of subducted oceanic crust along the CMB. Partial melting of subducted crust and other, hydrous subducted materials may also contribute to ULVZ variability.
Laboratory Earthquake Ruptures Contained by Velocity Strengthening Fault Patches
Authors: Song Jun Young , McLaskey Gregory C.
Published: April 2024

Many natural faults are believed to consist of velocity weakening (VW) patches surrounded by velocity strengthening (VS) sections. Numerical studies routinely employ this framework to study earthquake sequences including repeating earthquakes. In this laboratory study, we made a VW asperity, of length L, from a bare Poly(methyl methacrylate) PMMA frictional interface and coated the surrounding interface with Teflon to make VS fault sections. Behavior of this isolated asperity was studied as a function of L (ranging from 100 to 400 mm) and the critical nucleation length, , which is inversely proportional to the applied normal stress (2–16 MPa). Consistent with recent numerical simulations, we observed aseismic slip for  < 2, periodic slip for 2 <  < 6, and non‐periodic slip for 10 < . Furthermore, we compared the experiments where L was contained by VS material to standard stick‐slip events where L was bounded by free surfaces (i.e., L = the total sample length). The free surface case produced ∼10 times larger slip during stick‐slip events compared to the contained fault ruptures, even with identical . This disparity highlights how standard, complete‐rupture stick‐slip events differ from contained events expected in nature, due to both the free surface conditions and the heterogeneous normal stress along the fault near the free ends, as confirmed by Digital Image Correlation analysis. This study not only introduces the Teflon coating experimental technique for containing laboratory earthquake ruptures, but also highlights the utility of as a predictive parameter for earthquake behavior.
Climatology and Diurnal Variation of Ozone Column Abundances for 2.5 Mars Years as Measured by the NOMAD‐UVIS Spectrometer
Authors: Mason J. P. , Patel M. R. , Holmes J. A. , Wolff M. J. , Alday J. , Streeter P. , Olsen K. S. , Brown M. A. J. , Sellers G. , Marriner C. , Willame Y. , Thomas I. , Ristic B. , Daerden F. , Vandaele A. C. , Lopez‐Moreno J.‐J. , Bellucci G.
Published: April 2024

The distribution of Mars ozone (O3) is well established; however, our knowledge on the dayside diurnal variation of O3 is limited. We present measurements of Mars O3 column abundances, spanning Mars Year (MY) 34 to the end of MY 36, by the Ultraviolet and VIsible Spectrometer (UVIS), part of the Nadir and Occultation for MArs Discovery (NOMAD) instrument, aboard the ExoMars Trace Gas Orbiter. UVIS provides the capability to measure dayside diurnal variations of O3 and for the first time, a characterization of the dayside diurnal variations of O3 is attempted. The observed O3 climatology for Mars Years (MY) 34–36 follows the established seasonal trends observed through previous O3 measurements. At aphelion, the equatorial O3 distribution is observed to be strongly correlated with the water ice distribution. We show that the early dust storm in MY 35 resulted in a near‐global reduction in O3 during northern spring and the O3 abundances remained 14% lower in northern summer compared to MY36. Strong latitudinal and longitudinal variation was observed in the diurnal behavior of O3 around the northern summer solstice. In areas with a weak O3 upper layer, O3 column abundance peaks in the mid‐morning, driven by changes in the near‐surface O3 layer. In regions with greater O3 column abundances, O3 is observed to gradually increase throughout the day. This is consistent with the expected diurnal trend of O3 above the hygropause and suggests that in these areas an upper O3 layer persists throughout the Martian day.
Neuroimaging of posttraumatic stress disorder in adults and youth: progress over the last decade on three leading questions of the field
Authors: Hinojosa Cecilia A. , George Grace C. , Ben-Zion Ziv
Published: April 2024

Almost three decades have passed since the first posttraumatic stress disorder (PTSD) neuroimaging study was published. Since then, the field of clinical neuroscience has made advancements in understanding the neural correlates of PTSD to create more efficacious treatment strategies. While gold-standard psychotherapy options are available, many patients do not respond to them, prematurely drop out, or never initiate treatment. Therefore, elucidating the neurobiological mechanisms that define the disorder can help guide clinician decision-making and develop individualized mechanisms-based treatment options. To this end, this narrative review highlights progress made in the last decade in adult and youth samples on three outstanding questions in PTSD research: (1) Which neural alterations serve as predisposing (pre-exposure) risk factors for PTSD development, and which are acquired (post-exposure) alterations? (2) Which neural alterations can predict treatment outcomes and define clinical improvement? and (3) Can neuroimaging measures be used to define brain-based biotypes of PTSD? While the studies highlighted in this review have made progress in answering the three questions, the field still has much to do before implementing these findings into clinical practice. Overall, to better answer these questions, we suggest that future neuroimaging studies of PTSD should (A) utilize prospective longitudinal designs, collecting brain measures before experiencing trauma and at multiple follow-up time points post-trauma, taking advantage of multi-site collaborations/consortiums; (B) collect two scans to explore changes in brain alterations from pre-to-post treatment and compare changes in neural activation between treatment groups, including longitudinal follow up assessments; and (C) replicate brain-based biotypes of PTSD. By synthesizing recent findings, this narrative review will pave the way for personalized treatment approaches grounded in neurobiological evidence.
Polygenic scores for cardiovascular risk factors improve estimation of clinical outcomes in CCB treatment compared to pharmacogenetic variants alone
Authors: Türkmen Deniz , Bowden Jack , Masoli Jane A. H. , Delgado João , Kuo Chia-Ling , Melzer David , Pilling Luke C.
Published: April 2024

Pharmacogenetic variants are associated with clinical outcomes during Calcium Channel Blocker (CCB) treatment, yet whether the effects are modified by genetically predicted clinical risk factors is unknown. We analyzed 32,000 UK Biobank participants treated with dihydropiridine CCBs (mean 5.9 years), including 23 pharmacogenetic variants, and calculated polygenic scores for systolic and diastolic blood pressures, body fat mass, and other patient characteristics. Outcomes included treatment discontinuation and heart failure. Pharmacogenetic variant rs10898815-A (NUMA1) increased discontinuation rates, highest in those with high polygenic scores for fat mass. The RYR3 variant rs877087 T-allele alone modestly increased heart failure risks versus non-carriers (HR:1.13, p = 0.02); in patients with high polygenic scores for fat mass, lean mass, and lipoprotein A, risks were substantially elevated (HR:1.55, p = 4 × 10−5). Incorporating polygenic scores for adiposity and lipoprotein A may improve risk estimates of key clinical outcomes in CCB treatment such as treatment discontinuation and heart failure, compared to pharmacogenetic variants alone.
ELK3 destabilization by speckle-type POZ protein suppresses prostate cancer progression and docetaxel resistance
Authors: Lee Cheol-Jung , Lee Heejung , Kim Seo Ree , Nam Soo-Bin , Lee Ga-Eun , Yang Kyeong Eun , Lee Guk Jin , Chun Sang Hoon , Kang Han Chang , Lee Joo Young , Lee Hye Suk , Cho Sung-Jun , Cho Yong-Yeon
Published: April 2024

Accumulating evidence demonstrates that the activity regulation of ELK3, a member of the E26 transformation-specific oncogene family, is critical to regulating cell proliferation, migration, and survival in human cancers. However, the molecular mechanisms of how ELK3 induces chemoresistance in prostate cancer (PCa) have not been elucidated. In this study, we found that SPOP and ELK3 are an interacting partner. The interaction between SPOP and ELK3 resulted in increased ELK3 ubiquitination and destruction, assisted by checkpoint kinase-mediated ELK3 phosphorylation. Notably, the modulation of SPOP-mediated ELK3 protein stability affected the c-Fos-induced cell proliferation and invasion of PCa cells. The clinical involvement of the SPOP-ELK3 axis in PCa development was confirmed by an immunohistochemical assay on 123 PCa tissues, with an inverse correlation between increased ELK3 and decreased SPOP being present in ~80% of the specimens. This observation was supported by immunohistochemistry analysis using a SPOP-mutant PCa specimen. Finally, docetaxel treatment induced cell death by activating checkpoint kinase- and SPOP-mediated ELK3 degradation, while SPOP-depleted or SPOP-mutated PCa cells showed cell death resistance. Notably, this observation was correlated with the protein levels of ELK3. Taken together, our study reveals the precise mechanism of SPOP-mediated degradation of ELK3 and provides evidence that SPOP mutations contribute to docetaxel resistance in PCa.
Methane emission from a cool brown dwarf
Authors: Faherty Jacqueline K. , Burningham Ben , Gagné Jonathan , Suárez Genaro , Vos Johanna M. , Alejandro Merchan Sherelyn , Morley Caroline V. , Rowland Melanie , Lacy Brianna , Kiman Rocio , Caselden Dan , Kirkpatrick J. Davy , Meisner Aaron , Schneider Adam C. , Kuchner Marc Jason , Bardalez Gagliuffi Daniella Carolina , Beichman Charles , Eisenhardt Peter , Gelino Christopher R. , Gharib-Nezhad Ehsan , Gonzales Eileen , Marocco Federico , Rothermich Austin James , Whiteford Niall
Published: April 2024

Beyond our Solar System, aurorae have been inferred from radio observations of isolated brown dwarfs1,2. Within our Solar System, giant planets have auroral emission with signatures across the electromagnetic spectrum including infrared emission of H3
+ and methane. Isolated brown dwarfs with auroral signatures in the radio have been searched for corresponding infrared features, but only null detections have been reported3. CWISEP J193518.59-154620.3. (W1935 for short) is an isolated brown dwarf with a temperature of approximately 482 K. Here we report James Webb Space Telescope observations of strong methane emission from W1935 at 3.326 μm. Atmospheric modelling leads us to conclude that a temperature inversion of approximately 300 K centred at 1–10 mbar replicates the feature. This represents an atmospheric temperature inversion for a Jupiter-like atmosphere without irradiation from a host star. A plausible explanation for the strong inversion is heating by auroral processes, although other internal and external dynamical processes cannot be ruled out. The best-fitting model rules out the contribution of H3
+ emission, which is prominent in Solar System gas giants. However, this is consistent with rapid destruction of H3
+ at the higher pressure where the W1935 emission originates4.
The economic commitment of climate change
Authors: Kotz Maximilian , Levermann Anders , Wenz Leonie
Published: April 2024

Global projections of macroeconomic climate-change damages typically consider impacts from average annual and national temperatures over long time horizons1–6. Here we use recent empirical findings from more than 1,600 regions worldwide over the past 40 years to project sub-national damages from temperature and precipitation, including daily variability and extremes7,8. Using an empirical approach that provides a robust lower bound on the persistence of impacts on economic growth, we find that the world economy is committed to an income reduction of 19% within the next 26 years independent of future emission choices (relative to a baseline without climate impacts, likely range of 11–29% accounting for physical climate and empirical uncertainty). These damages already outweigh the mitigation costs required to limit global warming to 2 °C by sixfold over this near-term time frame and thereafter diverge strongly dependent on emission choices. Committed damages arise predominantly through changes in average temperature, but accounting for further climatic components raises estimates by approximately 50% and leads to stronger regional heterogeneity. Committed losses are projected for all regions except those at very high latitudes, at which reductions in temperature variability bring benefits. The largest losses are committed at lower latitudes in regions with lower cumulative historical emissions and lower present-day income.
Seismological evidence for a multifault network at the subduction interface
Authors: Chalumeau Caroline , Agurto-Detzel Hans , Rietbrock Andreas , Frietsch Michael , Oncken Onno , Segovia Monica , Galve Audrey
Published: April 2024

Subduction zones generate the largest earthquakes on Earth, yet their detailed structure, and its influence on seismic and aseismic slip, remains poorly understood. Geological studies of fossil subduction zones characterize the seismogenic interface as a 100 m–1 km thick zone1–3 in which deformation occurs mostly on metres-thick faults1,3–6. Conversely, seismological studies, with their larger spatial coverage and temporal resolution but lower spatial resolution, often image the seismogenic interface as a kilometres-wide band of seismicity7. Thus, how and when these metre-scale structures are active at the seismic-cycle timescale, and what influence they have on deformation is not known. Here we detect these metres-thick faults with seismicity and show their influence on afterslip propagation. Using a local three-dimensional velocity model and dense observations of more than 1,500 double-difference relocated earthquakes in Ecuador, we obtain an exceptionally detailed image of seismicity, showing that earthquakes occur sometimes on a single plane and sometimes on several metres-thick simultaneously active subparallel planes within the plate interface zone. This geometrical complexity affects afterslip propagation, demonstrating the influence of fault continuity and structure on slip at the seismogenic interface. Our findings can therefore help to create more realistic models of earthquake rupture, aseismic slip and earthquake hazard in subduction zones.
Hybrid speciation driven by multilocus introgression of ecological traits
Authors: Rosser Neil , Seixas Fernando , Queste Lucie M. , Cama Bruna , Mori-Pezo Ronald , Kryvokhyzha Dmytro , Nelson Michaela , Waite-Hudson Rachel , Goringe Matt , Costa Mauro , Elias Marianne , Mendes Eleres de Figueiredo Clarisse , Freitas André Victor Lucci , Joron Mathieu , Kozak Krzysztof , Lamas Gerardo , Martins Ananda R. P. , McMillan W. Owen , Ready Jonathan , Rueda-Muñoz Nicol , Salazar Camilo , Salazar Patricio , Schulz Stefan , Shirai Leila T. , Silva-Brandão Karina L. , Mallet James , Dasmahapatra Kanchon K.
Published: April 2024

Hybridization allows adaptations to be shared among lineages and may trigger the evolution of new species1,2. However, convincing examples of homoploid hybrid speciation remain rare because it is challenging to demonstrate that hybridization was crucial in generating reproductive isolation3. Here we combine population genomic analysis with quantitative trait locus mapping of species-specific traits to examine a case of hybrid speciation in Heliconius butterflies. We show that Heliconius elevatus is a hybrid species that is sympatric with both parents and has persisted as an independently evolving lineage for at least 180,000 years. This is despite pervasive and ongoing gene flow with one parent, Heliconius pardalinus, which homogenizes 99% of their genomes. The remaining 1% introgressed from the other parent, Heliconius melpomene, and is scattered widely across the H. elevatus genome in islands of divergence from H. pardalinus. These islands contain multiple traits that are under disruptive selection, including colour pattern, wing shape, host plant preference, sex pheromones and mate choice. Collectively, these traits place H. elevatus on its own adaptive peak and permit coexistence with both parents. Our results show that speciation was driven by introgression of ecological traits, and that speciation with gene flow is possible with a multilocus genetic architecture.
Control of working memory by phase–amplitude coupling of human hippocampal neurons
Authors: Daume Jonathan , Kamiński Jan , Schjetnan Andrea G. P. , Salimpour Yousef , Khan Umais , Kyzar Michael , Reed Chrystal M. , Anderson William S. , Valiante Taufik A. , Mamelak Adam N. , Rutishauser Ueli
Published: April 2024

Retaining information in working memory is a demanding process that relies on cognitive control to protect memoranda-specific persistent activity from interference1,2. However, how cognitive control regulates working memory storage is unclear. Here we show that interactions of frontal control and hippocampal persistent activity are coordinated by theta–gamma phase–amplitude coupling (TG-PAC). We recorded single neurons in the human medial temporal and frontal lobe while patients maintained multiple items in their working memory. In the hippocampus, TG-PAC was indicative of working memory load and quality. We identified cells that selectively spiked during nonlinear interactions of theta phase and gamma amplitude. The spike timing of these PAC neurons was coordinated with frontal theta activity when cognitive control demand was high. By introducing noise correlations with persistently active neurons in the hippocampus, PAC neurons shaped the geometry of the population code. This led to higher-fidelity representations of working memory content that were associated with improved behaviour. Our results support a multicomponent architecture of working memory1,2, with frontal control managing maintenance of working memory content in storage-related areas3–5. Within this framework, hippocampal TG-PAC integrates cognitive control and working memory storage across brain areas, thereby suggesting a potential mechanism for top-down control over sensory-driven processes.
Control of neuronal excitation–inhibition balance by BMP–SMAD1 signalling
Authors: Okur Zeynep , Schlauri Nadia , Bitsikas Vassilis , Panopoulou Myrto , Ortiz Raul , Schwaiger Michaela , Karmakar Kajari , Schreiner Dietmar , Scheiffele Peter
Published: April 2024

Throughout life, neuronal networks in the mammalian neocortex maintain a balance of excitation and inhibition, which is essential for neuronal computation1,2. Deviations from a balanced state have been linked to neurodevelopmental disorders, and severe disruptions result in epilepsy3–5. To maintain balance, neuronal microcircuits composed of excitatory and inhibitory neurons sense alterations in neural activity and adjust neuronal connectivity and function. Here we identify a signalling pathway in the adult mouse neocortex that is activated in response to increased neuronal network activity. Overactivation of excitatory neurons is signalled to the network through an increase in the levels of BMP2, a growth factor that is well known for its role as a morphogen in embryonic development. BMP2 acts on parvalbumin-expressing (PV) interneurons through the transcription factor SMAD1, which controls an array of glutamatergic synapse proteins and components of perineuronal nets. PV-interneuron-specific disruption of BMP2–SMAD1 signalling is accompanied by a loss of glutamatergic innervation in PV cells, underdeveloped perineuronal nets and decreased excitability. Ultimately, this impairment of the functional recruitment of PV interneurons disrupts the cortical excitation–inhibition balance, with mice exhibiting spontaneous epileptic seizures. Our findings suggest that developmental morphogen signalling is repurposed to stabilize cortical networks in the adult mammalian brain.
Immobilized lipase enzyme on green synthesized magnetic nanoparticles using Psidium guava leaves for dye degradation and antimicrobial activities
Authors: Fahim Yosri A. , El-Khawaga Ahmed M. , Sallam Reem M. , Elsayed Mohamed A. , Assar Mohamed Farag Ali
Published: April 2024

Zinc ferrite nanoparticles (ZnF NPs) were synthesized by a green method using Psidium guava Leaves extract and characterized via structural and optical properties. The surface of ZnF NPs was stabilized with citric acid (CA) by a direct addition method to obtain (ZnF-CA NPs), and then lipase (LP) enzyme was immobilized on ZnF-CA NPs to obtain a modified ZnF-CA-LP nanocomposite (NCs). The prepared sample’s photocatalytic activity against Methylene blue dye (MB) was determined. The antioxidant activity of ZnF-CA-LP NCs was measured using 1,1-diphenyl-2-picryl hydrazyl (DPPH) as a source of free radicals. In addition, the antibacterial and antibiofilm capabilities of these substances were investigated by testing them against gram-positive Staphylococcus aureus (S. aureus ATCC 25923) and gram-negative Escherichia coli (E. coli ATCC 25922) bacterial strains. The synthesized ZnF NPs were discovered to be situated at the core of the material, as determined by XRD, HRTEM, and SEM investigations, while the CA and lipase enzymes were coated in this core. The ZnF-CA-LP NCs crystallite size was around 35.0 nm at the (311) plane. Results obtained suggested that 0.01 g of ZnF-CA-LP NCs achieved 96.0% removal of 5.0 ppm of MB at pH 9.0. In-vitro zone of inhibition (ZOI) and minimum inhibitory concentration (MIC) results verified that ZnF-CA-LP NCs exhibited its encouraged antimicrobial activity against S. aureus and E. coli (20.0 ± 0.512, and 27.0 ± 0.651 mm ZOI, respectively) & (1.25, and 0.625 μg/ml MIC, respectively). ZnF-CA-LP NPs showed antibiofilm percentage against S. aureus (88.4%) and E. coli (96.6%). Hence, ZnF-CA-LP NCs are promising for potential applications in environmental and biomedical uses.
Comparative study of eco-friendly wire mesh configurations to enhance sustainability in reinforced concrete structures
Authors: Mebrahtom Misgina , Fissha Yewuhalashet , Ali Mujahid , Gebretsadik Angesom , Kide Yemane , Nguse Zaid , Gebrehiwot Zemicael , Flores Erick Saavedra , Avudaiappan Siva , Ikeda Hajime
Published: April 2024

Recent and past studies mainly focus on reducing the dead weight of structure; therefore, they considered lightweight aggregate concrete (LWAC) which reduces the dead weight but also affects the strength parameters. Therefore, the current study aims to use varied steel wire meshes to investigate the effects of LWAC on mechanical properties. Three types of steel wire mesh are used such as hexagonal (chicken), welded square, and expanded metal mesh, in various layers and orientations in LWAC. Numerous mechanical characteristics were examined, including energy absorption (EA), compressive strength (CS), and flexural strength (FS). A total of ninety prisms and thirty-three cubes were made. For the FS test, forty-five 100 × 100 × 500 mm prism samples were poured, thirty-three 150 × 150 × 150 mm cube samples were made, and forty-five 400 × 300 × 75 mm EA specimens were costed for fourteen days of curing. The experimental findings demonstrate that the FS was enhanced by adding additional forces that spread the forces over the section. One layer of chicken, welded, and expanded metal mesh enhances the FS by 52.96%, 23.76%, and 22.2%, respectively. In comparison to the remaining layers, the FS in a single-layer hexagonal wire mesh has the maximum strength, 29.49 MPa. The hexagonal wire mesh with a single layer had the greatest CS, measuring 36.56 MPa. When all three types of meshes are combined, the CS does not vary in this way and is estimated to be 29.79 MPa. In the combination of three layers, the chicken and expanded wire mesh had the most energy recorded prior to final failure, which was 1425.6 and 1108.7 J, whereas it was found the highest 752.3 J for welded square wire mesh. The energy absorption for the first layer with hexagonal wire mesh increased by 82.81% prior to the crack and by 88.34% prior to the ultimate failure. Overall, it was determined and suggested that hexagonal wire mesh works better than expanded and welded wire meshes.
The prognostic value of sialylation-related long non-coding RNAs in lung adenocarcinoma
Authors: Wang Beiru , Hou Chengyu , Yu Xiang , Liu Jiaxin , Wang Jiyong
Published: April 2024

There has been increasing interest in the role of epigenetic modification in cancers recently. Among the various modifications, sialylation has emerged as a dominant subtype implicated in tumor progression, metastasis, immune evasion, and chemoresistance. The prognostic significance of sialylation-related molecules has been demonstrated in colorectal cancer. However, the potential roles and regulatory mechanisms of sialylation in lung adenocarcinoma (LUAD) have not been thoroughly investigated. Through Pearson correlation, univariate Cox hazards proportional regression, and random survival forest model analyses, we identified several prognostic long non-coding RNAs (lncRNAs) associated with aberrant sialylation and tumor progression, including LINC00857, LINC00968, LINC00663, and ITGA9-AS1. Based on the signatures of four lncRNAs, we classified patients into two clusters with different landscapes using a non-negative matrix factorization approach. Collectively, patients in Cluster 1 (C1) exhibited worse prognoses than those in Cluster 2 (C2), as well as heavier tumor mutation burden. Functional enrichment analysis showed the enrichment of several pro-tumor pathways in C1, differing from the upregulated Longevity and programmed cell death pathways in C2. Moreover, we profiled immune infiltration levels of important immune cell lineages in two subgroups using MCPcounter scores and single sample gene set enrichment analysis scores, revealing a relatively immunosuppressive microenvironment in C1. Risk analysis indicated that LINC00857 may serve as a pro-tumor regulator, while the other three lncRNAs may be protective contributors. Consistently, we observed upregulated LINC00857 in C1, whereas increased expressive levels of LINC00968, LINC00663, and ITGA9-AS1 were observed in C2. Finally, drug sensitivity analysis suggested that patients in the two groups may benefit from different therapeutic strategies, contributing to precise treatment in LUAD. By integrating multi-omics data, we identified four core sialylation-related lncRNAs and successfully established a prognostic model to distinguish patients with different characterizations. These findings may provide some insights into the underlying mechanism of sialylation, and offer a new stratification way as well as clinical guidance in LUAD.
Simple and inexpensive microwave setup for industrial based applications: Quantification of flower honey adulteration as a case study
Authors: Hasar Ugur C. , Hasar Hafize , Ozturk Hamdullah , Korkmaz Huseyin , Kaya Yunus , Ozkaya Mehmet Akif , Ebrahimi Amir , Barroso Joaquim J. , Nayyeri Vahid , Ramahi Omar M.
Published: April 2024

A simple and inexpensive microwave measurement setup based on measurements of magnitudes of transmission properties ($$|S_{21}|_{\text {dB}}$$

|

S
21

|

dB

) is proposed for industrial-based microwave aquametry (moisture or water content) applications. An easy-to-apply calibration procedure based on normalization is implemented to eliminate systematic errors in the measurement system. As a case study, we applied this setup for the quantification of water-adulteration in flower honey. After validating this system by distilled water and pure flower honey measurements, $$|S_{21}|_{\text {dB}}$$

|

S
21

|

dB

measurements of the pure flower honey with various adulteration percentages ($$\delta$$
δ
) up to 9% are conducted to examine the performance of the measurement setup for quantification of water adulteration. A multi-dimensional fitting procedure is implemented to predict $$\delta$$
δ
using the proposed inexpensive microwave measurement setup. It is shown that it is possible to quantify an adulteration level with an accuracy better than $${\mp } 1$$

∓
1

% by the proposed measurement setup and the applied multi-dimensional fitting procedure.
Convolutional spiking neural networks for intent detection based on anticipatory brain potentials using electroencephalogram
Authors: Lutes Nathan , Nadendla Venkata Sriram Siddhardh , Krishnamurthy K.
Published: April 2024

Spiking neural networks (SNNs) are receiving increased attention because they mimic synaptic connections in biological systems and produce spike trains, which can be approximated by binary values for computational efficiency. Recently, the addition of convolutional layers to combine the feature extraction power of convolutional networks with the computational efficiency of SNNs has been introduced. This paper studies the feasibility of using a convolutional spiking neural network (CSNN) to detect anticipatory slow cortical potentials (SCPs) related to braking intention in human participants using an electroencephalogram (EEG). Data was collected during an experiment wherein participants operated a remote-controlled vehicle on a testbed designed to simulate an urban environment. Participants were alerted to an incoming braking event via an audio countdown to elicit anticipatory potentials that were measured using an EEG. The CSNN’s performance was compared to a standard CNN, EEGNet and three graph neural networks via 10-fold cross-validation. The CSNN outperformed all the other neural networks, and had a predictive accuracy of 99.06% with a true positive rate of 98.50%, a true negative rate of 99.20% and an F1-score of 0.98. Performance of the CSNN was comparable to the CNN in an ablation study using a subset of EEG channels that localized SCPs. Classification performance of the CSNN degraded only slightly when the floating-point EEG data were converted into spike trains via delta modulation to mimic synaptic connections.
Changes in concentration performance and alternating attention after short-term virtual reality training in E-athletes: a pilot study
Authors: Lachowicz Maciej , Żurek Alina , Jamro Dariusz , Serweta-Pawlik Anna , Żurek Grzegorz
Published: April 2024

In the dynamic landscape of e-sports, where intense competitive gaming demands high cognitive abilities, concentration performance and alternating attention play a pivotal role. E-sports encompass diverse genres, each requiring specific cognitive functions. Maintaining unwavering focus is crucial, as split-second decisions can determine victory. The study explores the potential of Virtual Reality (VR) training to enhance concentration performance and alternating attention, shedding light on the importance and possibilities of optimizing cognitive abilities for e-athletes. VR training emerges as a promising intervention, offering immersive environments for cognitive exercises. The study investigates the impact of VR training on concentration performance and alternating attention in amateur e-athletes, utilizing standardized tests. A randomized controlled trial with 66 participants reveals significant improvements in the VR training group, highlighting the adaptability and plasticity of cognitive processes. The findings suggest that VR training can enhance concentration abilities, providing valuable insights for e-sports and potentially extending to other fields requiring sustained attention and rapid task-switching. The study underscores the convergence of cognitive psychology, neuroscience, and VR technology, paving the way for innovative training methodologies and advancements in e-sports performance.
Clinical characteristics and cytological changes in mucinous obstruction diagnosed by dacryoendoscopy
Authors: Kim Doah , Lew Helen
Published: April 2024

To analyze the clinical characteristics of mucinous obstruction diagnosed by dacryoendoscopy and compared the cytological changes with membranous obstruction using a modified liquid-based thin prep cytology method. A retrospective chart review was conducted on 53 eyes of 51 patients with mucus obstruction based on dacryoendoscopic findings from January 2022 to October 2022. Liquid-based thin-prep cytology was performed by irrigating the inside of the nasolacrimal drainage system with saline during dacryoendoscopy-guided silicone tube intubation. Pathological findings were analyzed through a comparison of mucinous obstruction with membranous obstruction as determined by dacryoendoscopic findings. The modified liquid-based thin prep cytology technique had a higher cytology detection rate across all cases. Mucinous obstruction exhibited a significantly higher number of successful canalicular irrigation test cases compared to membranous obstruction. In mucinous obstruction, epithelial squamous cells were more frequently detected in pre-sac obstruction, whereas columnar epithelial cells were predominant in post-sac obstruction. Inflammatory cells showed a stronger correlation with primary change and post-sac obstruction. Bacterial colonies were observed exclusively in cases of mucinous obstruction. The use of a modified liquid-based thin prep cytology method enables the examination of histopathological changes in the lacrimal passage in primary acquired nasolacrimal duct obstruction (PANDO), particularly in cases of mucinous obstruction, without the need for invasive biopsies. These findings enhance the understanding of the etiopathogenesis of mucinous obstruction, complementing knowledge of membranous obstruction in PANDO.
Coprophagia in early life tunes expression of immune genes after weaning in rabbit ileum
Authors: Cauquil L. , Beaumont M. , Schmaltz-Panneau B. , Liaubet L. , Lippi Y. , Naylies C. , Bluy L. , Poli M. , Gress L. , Lencina C. , Duranthon V. , Combes S.
Published: April 2024

Coprophagia by suckling rabbits, i.e. ingestion of feces from their mother, reduces mortality after weaning. We hypothesized that this beneficial effect of coprophagia is immune-mediated at the intestinal level. Therefore, this study investigated immune development after weaning by analyzing the ileal transcriptome at day 35 and 49 in rabbits with differential access to coprophagia in early life. Rabbit pups had access between day 1 and 15 to (i) no feces (NF) or (ii) feces from unrelated does (Foreign Feces, FF) or (iii) feces from unrelated does treated with antibiotics (FFab). 350 genes were differentially expressed between day 35 and day 49 in suckling rabbits with access to coprophagia. These genes coded for antimicrobial peptides, a mucin, cytokines and chemokines, pattern recognition receptors, proteins involved in immunoglobulin A secretion and in interferon signaling pathway. Strikingly, prevention of coprophagia or access to feces from antibiotic-treated does in early life blunted immune development between day 35 et 49 in the ileum of rabbits. Thus, coprophagia might be crucial for the maturation of intestinal immunity in rabbits and could explain why this behavior improves survival.
The influence of electrical high-speed rotation on mandibular third molar surgeries: a prospective, randomized, split-mouth clinical and radiographic study
Authors: Sol Izabella , Tonini Karen Rawen , dos Reis Karen Santin , Hadad Henrique , Ponzoni Daniela
Published: April 2024

The aim of this split-mouth randomized clinical trial was to evaluate the clinical outcomes (operative time, edema, trismus, and pain), the immediate histological effects, the alveolar repair (2 and 4 months), and the quality of life after the extraction of impacted third molars using high-speed pneumatic and electrical rotation. Sixteen patients underwent extraction of the two mandibular third molars with a minimum interval of 15 days. On one side of the participant’s mouth, high-speed pneumatic rotation was used (Control Group—CG) while for the other side, high-speed electrical rotation was used (Study Group—SG). Statistical analysis included ANOVA repeated measures and Pearson correlations. SG group showed: shorter operative time (p = 0.019), less pain (p = 0.034), swelling (p < 0.001) and trismus (p = 0.025) on the 1st postoperative day; less pain (p = 0.034) and trismus (p = 0.010) on the 3rd postoperative day; less trismus (p = 0.032) on the 7th postoperative day; and better quality of life (p = 0.007). No differences were observed for peripheral bone damage or bone density of alveolar repair at 2 and 4 months between groups. Electric high-speed rotation provided better postoperative clinical parameters of pain, edema and trismus when compared with pneumatic high-speed rotation for mandibular third molar surgery.

Trial registration: Brazilian Registry of Clinical Trials registration number RBR-4xyqhqm (https://ensaiosclinicos.gov.br/rg/RBR-4xyqhqm).