bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Modulation of Heat-Inducible Genes Across Diverse Genotypes and Molecular Cloning of a sHSP From Pearl Millet [Pennisetum glaucum (L.) R. Br.]
Authors: Mukesh Sankar S. , Tara Satyavathi C. , Barthakur Sharmistha , Singh Sumer Pal , Bharadwaj C. , Soumya S. L.
Published: July 2021

The survival, biomass, and grain yield of most of the crops are negatively influenced by several environmental stresses. The present study was carried out by using transcript expression profiling for functionally clarifying the role of genes belonging to a small heat shock protein (sHSP) family in pearl millet under high-temperature stress. Transcript expression profiling of two high-temperature-responsive marker genes, Pgcp70 and PgHSF, along with physio-biochemical traits was considered to screen out the best contrasting genotypes among the eight different pearl millet inbred lines in the seedling stage. Transcript expression pattern suggested the existence of differential response among different genotypes upon heat stress in the form of accumulation of heat shock-responsive gene transcripts. Genotypes, such as WGI 126, TT-1, TT-6, and MS 841B, responded positively toward high-temperature stress for the transcript accumulation of both Pgcp70 and PgHSF and also indicated a better growth under heat stress. PPMI-69 showed the least responsiveness to transcript induction; moreover, it supports the membrane stability index (MSI) data for scoring thermotolerance, thereby suggesting the efficacy of transcript expression profiling as a molecular-based screening technique for the identification of thermotolerant genes and genotypes at particular crop growth stages. The contrasting genotypes, such as PPMI-69 (thermosusceptible) and WGI-126 and TT-1 (thermotolerant), are further utilized for the characterization of thermotolerance behavior of sHSP by cloning a PgHSP16.97 from the thermotolerant cv. WGI-126. In addition, the investigation was extended for the identification and characterization of 28 different HSP20 genes through a genome-wide search in the pearl millet genome and an understanding of their expression pattern using the RNA-sequencing (RNA-Seq) data set. The outcome of the present study indicated that transcript profiling can be a very useful technique for high-throughput screening of heat-tolerant genotypes in the seedling stage. Also, the identified PgHSP20s genes can provide further insights into the molecular regulation of pearl millet stress tolerance, thereby bridging them together to fight against the unpredicted nature of abiotic stress.
Alternative Measures of Political Efficacy: The Quest for Cross-Cultural Invariance With Ordinally Scaled Survey Items
Authors: Scotto Thomas J. , Xena Carla , Reifler Jason
Published: July 2021

In this paper, we examine the measurement of citizens’ beliefs that politicians and political systems are responsive (external efficacy) and that citizens see themselves sufficiently skilled to participate in politics (internal efficacy). This paper demonstrates techniques that allow researchers to establish the cross-context validity of conceptually important ordinal scales. In so doing, we show an alternative set of efficacy indicators to those commonly appearing on cross-national surveys to be more promising from a validity standpoint. Through detailed discussion and application of multi-group analysis for ordinal measures, we demonstrate that a measurement model linking latent internal and external efficacy factors performs well in configural and parameter invariance testing when applied to representative samples of respondents in the United States and Great Britain. With near full invariance achieved, differences in latent variable means are meaningful and British respondents are shown to have lower levels of both forms of efficacy than their American counterparts. We argue that this technique may be particularly valuable for scholars who wish to establish the suitability of ordinal scales for direct comparison across nations or cultures.
Disease Avoidance Model Explains the Acceptance of Cohabitation With Bats During the COVID-19 Pandemic
Authors: Lundberg Piia , Ojala Ann , Suominen Kati M. , Lilley Thomas , Vainio Annukka
Published: July 2021

Bats and humans have a close relationship based on cohabitation, with bats taking roost in buildings. It has been suggested that bats function as a reservoir of the SARS-CoV-2 virus that causes the COVID-19 disease in humans. A misconception that bats can spread SARS-CoV-2 to humans may have increased negative emotions toward bats and reduced individuals’ acceptance of cohabitation with bats during the COVID-19 pandemic. By applying the disease avoidance model, we tested whether knowledge about bats would be associated with reduced negative emotions toward bats, which in turn would be associated with increased acceptance of cohabitation with bats. Moreover, we tested whether previous experiences of bats, perceived COVID-19 risk, age, gender and level of education would be associated with negative emotions and acceptance of bats. A quantitative survey (N= 577) collected during the COVID-19 pandemic in Finland was analyzed with multiple linear regression. The results supported the disease avoidance model. Negative emotions toward bats reduced the acceptance of cohabitation with bats. However, knowledge about bats was associated with increased acceptance of bats both directly, as well as indirectly,viareduced negative emotions. Moreover, perceived COVID-19 risk was associated with increased negative emotions toward bats, and reduced acceptance of bats. Females were more likely than other respondents to report negative emotions, and reduced acceptance of cohabitation with bats. Prior experience of bats was associated with increased acceptance of bats as neighbors. These findings suggest that COVID-19 pandemic may threaten the existence of bats if no action is taken. The findings highlight the importance of correcting misunderstandings about non-human species as transmitters of diseases to humans.
Low Altruism as a Cause of Ostracism
Authors: Huang Lianqiong , Zhu Yingge , Zhang Denghao
Published: July 2021

This study focused on the causes of ostracism and explored the relationship between altruistic personality traits and ostracism. Using a combination of questionnaire surveys and laboratory experiments, results showed that: individuals with lower altruism were more vulnerable to be ostracized than those with higher altruism (Study 1 and Study 2). The relationship between altruism and ostracism was partially mediated by social responsibility (Study 3). When facing a low-altruistic target, the source would infer that the social responsibility level of this target was also low, thereby leading the ostracism intention to the target. Empathy did not moderate the relationship between altruism and ostracism. On the one hand, empathy did not moderate the direct effect of altruism on ostracism (Study 2); on the other hand, it also did not moderate the mediating role of social responsibility (Study 3). The strengths and limitations of this research are also discussed.
Normal Face Detection Over a Range of Luminance Contrasts in Adolescents With Autism Spectrum Disorder
Authors: Norton Daniel J. , McBain Ryan K. , Murray Grace E. , Khang Juna , Zong Ziqing , Bollacke Hannah R. , Maher Stephen , Levy Deborah L. , Ongur Dost , Chen Yue
Published: July 2021

Face recognition is impaired in autism spectrum disorders (ASDs), but the reason for this remains unclear. One possibility is that impairments in the ability to visually detect faces might be a factor. As a preliminary study in this vein, we measured face detection ability as a function of visual contrast level in 13 individuals with ASD, aged 13–18, and 18 neurotypical controls (NCs) in the same age range. We also measured contrast sensitivity, using sinusoidal grating stimuli, as a control task. Individuals with ASD did not differ from controls in face detection (p > 0.9) or contrast detection (p > 0.2) ability. Performance on contrast and face detection was significantly correlated in ASD but not in NC. Results suggest that the ability to visually detect faces is not altered in ASD overall, but that alterations in basic visual processing may affect face detection ability in some individuals with ASD.
Anxiety-Depressive Syndrome and Binge-Watching Among Young Adults
Authors: Starosta Jolanta , Izydorczyk Bernadetta , Wontorczyk Antoni
Published: July 2021

In recent years, binge-watching becomes a highly popular way of spending free time. Even though binge-watching usually is related to entertainment, there are concerns about some negative and unhealthy outcomes of excessive form of this behavior. The study examined the predictive value of anxiety-depressive syndrome in explaining the symptoms of problematic binge-watching and the tendency to adopt a specific motivation to watch series. Research group consists of 645 Polish young adults. The State-Trait Anxiety Inventory, Depression Measurement Questionnaire, Viewing Motivation Scale, and Questionnaire of Excessive Binge-Watching were used in this study. The results of the path analysis show that anxiety-depressive syndrome and motivation to watch TV series are the significant factors in the manifestation of all symptoms of problematic binge-watching. Moreover, there is a significant relation between anxiety-depressive syndrome and motivation to watch TV series, which especially concerns escape motivation and motivation to deal with loneliness. Furthermore, motivation to deal with loneliness, escape motivation, and motivation to spend free time have mediating effect on the relationship between anxiety-depressive syndrome and problematic binge-watching. Results of this research show that there could be not only normative binge-watching behavior but also unhealthy and problematic form of this behavior.
The Multivariable Multiaxial Suggestibility Inventory-2 (MMSI-2): A Psychometric Alternative to Measure and Explain Supernatural Experiences
Authors: Escolà-Gascón Álex , Dagnall Neil , Gallifa Josep
Published: July 2021

This paper presents the English adaptation of the Multivariable Multiaxial Suggestibility Inventory-2 (MMSI-2), a questionnaire developed specifically for psychological assessment and prediction of anomalous phenomena. The sample consisted of 613 respondents from England (47.6% were women and 52.4% men). All of them were of legal age (mean = 34.5; standard deviation = 8.15). An exploratory factor analysis was applied, and three confirmatory factor models were adjusted. Omega coefficients and test-retest designs were used for reliability analysis. The MMSI-2 has a valid internal structure consisting of five macrofactors: Clinical Personality Tendencies (CPT), Anomalous Perceived Phenomena (APP), Incoherent Manipulations (IMA), Altered States of Consciousness (ASC), and Openness (OP). Omega coefficients for CPT and OP factors were low but acceptable. Furthermore, test-retest trials were excellent for all scales and factors. The psychological factors CPT, IMA, and ASC predicted 18.3% of the variance of anomalous experiences (APP). The authors concluded the English MMSI-2 was a valid and reliable test for the evaluation of anomalous phenomena but recommend that subsequent research reviews the predictive quality of the underlying model.
Exploring the Potential Impact of a Virtual Body Scan Meditation Exercise Conducted With Pet Dogs on Recipients and Facilitators
Authors: Kogan Lori R. , Bussolari Cori
Published: July 2021

Numerous recent studies have shown that COVID-19 and the accompanying mandated lifestyle changes have resulted in significant negative effects on people’s mental health. To meet the increased need for mental health support, while also maintaining physical safety, a variety of telehealth services have been created or expanded. A body scan mindfulness program is an intervention that can easily be modified to be offered virtually. This study was designed to determine if a virtual body scan mindfulness exercise, with participants’ holding their dog or a pillow/blanket, could reduce their stress and anxiety as well as that of the facilitators. Significant differences in pre/post-State Anxiety Assessment scores for participants and facilitators were found. These results are discussed within the framework of the human animal bond and the potential of this form of intervention as a useful virtual tool for participants and facilitators alike.
The Relationship Between Chinese English Major Students' Learning Anxiety and Enjoyment in an English Language Classroom: A Positive Psychology Perspective
Authors: Fang Fan , Tang Xiaofei
Published: July 2021

Many studies have explored learner psychology in relation to second language acquisition (SLA) in order to understand the effectiveness and difficulties of language learning. In the last two decades, emotional factors in students' language learning have garnered much attention in the field of SLA. However, more recently, studies have begun to focus on enjoyment and its relationship with anxiety. By collecting data at a provincial key university in southeast China, the study discussed in this paper investigated English major university students' emotions related to learning English. By collecting questionnaire responses from 140 English major undergraduates and conducting interviews with six students, the findings revealed that the participants' levels of foreign language enjoyment (FLE) were significantly higher than their levels of foreign language classroom anxiety (FLCA) and that they experienced FLE more frequently than FLCA. It was also found that the participants' FLE was more related to their teachers and peers and their FLCA was more related to their emotions, such as fear of a negative evaluation and speaking without sufficient preparation. In addition, this study also provides a few pedagogical implications for improving foreign language learning outcomes and teaching efficiency in English teaching and learning.
Lifestyle Interventions Improving Cannabinoid Tone During COVID-19 Lockdowns May Enhance Compliance With Preventive Regulations and Decrease Psychophysical Health Complications
Authors: Brugnatelli Viola , Facco Enrico , Zanette Gastone
Published: July 2021

Studies investigating the psychosomatic effects of social isolation in animals have shown that one of the physiologic system that gets disrupted by this environment-affective change is the Endocannabinoid System. As the levels of endocannabinoids change in limbic areas and prefrontal cortex during stressful times, so is the subject more prone to fearful and negative thoughts and aggressive behavior. The interplay of social isolation on the hypothalamic-pituitary-adrenal axis and cannabinoid tone triggers a vicious cycle which further impairs the natural body's homeostatic neuroendocrine levels and provokes a series of risk factors for developing health complications. In this paper, we explore the psychosomatic impact of prolonged quarantine in healthy individuals, and propose management and coping strategies that may improve endocannabinoid tone, such as integration of probiotics, cannabidiol, meditation, and physical exercise interventions with the aim of supporting interpersonal, individual, and professional adherence with COVID-19 emergency public measures whilst minimizing their psycho-physical impact.
mRNA Levels of MAOA and 5-HT2A Receptor in Patients With Pathological Internet Use: Correlations With Comorbid Symptoms
Authors: Qiu Mimi , Zhang Chenchen , Dai Yu , Zhang Lingrui , Wang Yang , Peng Wei , Chen Yalin , Wen Chao , Li Hui , Zhu Tianmin
Published: July 2021

Objective: Uncontrolled internet use may lead to the emergence of pathological internet use (PIU). PIU has become a global public health concern that can cause a range of psychotic symptoms, including anxiety, depression, and impulse control disorder. To date, we know very little about the principal biological factors related to PIU. Monoamine oxidase type A (MAOA) and serotonin (5-HT) 5-HT2A receptor (5-HT2AR) play critical roles in the development of behavioural and drug addictions. Thus, the aim of this study was to measure the relative expression of mRNA of MAOA and 5-HT2AR in peripheral blood mononuclear cells (PBMCs) of patients with PIU and to determine the correlations between these biological indicators and the comorbid symptoms of patients with PIU.

Methods: In this study, the mRNA of MAOA and 5-HT2AR was detected using real-time PCR in PBMCs of the patients with PIU (n = 24) and healthy controls (HCs, n = 25). The relationship between the mRNA levels of MAOA and 5-HT2AR and clinical symptoms in patients with PIU was further investigated.

Results: MAOA mRNA in PBMCs was significantly upregulated in patients with PIU compared with that in HCs. mRNA levels of 5-HT2AR were not found to differ significantly between HCs and patients with PIU. Correlation analyses further revealed a significant positive correlation between the relative expression of MAOA mRNA in PBMCs of patients with PIU and the Young's Internet Addiction Test and Self-Rating Depression Scale scores.

Conclusion: The present study revealed upregulated expression of MAOA mRNA in patients with PIU and an association between the expression of MAOA mRNA and clinical symptoms of PIU, suggesting that the neurobiological changes may be similar between PIU and substance addiction. Additionally, this study demonstrated a potential association between comorbid symptoms and mRNA levels of MAOA.
Heterogeneity Within Youth With Childhood-Onset Conduct Disorder in the ABCD Study
Authors: Brislin Sarah J. , Martz Meghan E. , Cope Lora M. , Hardee Jillian E. , Weigard Alexander , Heitzeg Mary M.
Published: July 2021

The purpose of this study was to examine if personality traits can be used to characterize subgroups of youth diagnosed with childhood-onset conduct disorder (CD). Participants were 11,552 youth from the Adolescent Brain Cognitive Development study. Data used in this report came from doi: 10.15154/1504041 (M age 9.92; 45.3% female, 49.6% white, 19.0% Hispanic). A subset of this sample (n = 365) met criteria for CD. Latent profile analyses (LPA) were performed on this subgroup (n = 365) to define profiles of individuals with CD based on self-report measures of impulsivity, punishment sensitivity, reward response, and callous-unemotional traits. Follow up analyses determined if these groups differed on clinically relevant variables including psychopathology, environmental risk factors, social risk factors, and neurocognitive functioning. Participants with a CD diagnosis scored significantly higher on psychological, environmental, social, and neurocognitive risk factors. The LPA revealed three unique profiles, which differed significantly on liability for broad psychopathology and domain-specific liability for externalizing psychopathology but were largely matched on environmental and social risk factors. These unique configurations provide a useful way to further parse clinically relevant subgroups within youth who meet criteria for childhood-onset CD, setting the stage for prospective longitudinal research using these latent profiles to better understand the development of youth with childhood-onset CD.
Excess Mortality in Italy During the COVID-19 Pandemic: Assessing the Differences Between the First and the Second Wave, Year 2020
Authors: Dorrucci Maria , Minelli Giada , Boros Stefano , Manno Valerio , Prati Sabrina , Battaglini Marco , Corsetti Gianni , Andrianou Xanthi , Riccardo Flavia , Fabiani Massimo , Vescio Maria Fenicia , Spuri Matteo , Urdiales Alberto Mateo , Martina Del Manso , Onder Graziano , Pezzotti Patrizio , Bella Antonino
Published: July 2021

COVID-19 dramatically influenced mortality worldwide, in Italy as well, the first European country to experience the Sars-Cov2 epidemic. Many countries reported a two-wave pattern of COVID-19 deaths; however, studies comparing the two waves are limited. The objective of the study was to compare all-cause excess mortality between the two waves that occurred during the year 2020 using nationwide data. All-cause excess mortalities were estimated using negative binomial models with time modeled by quadratic splines. The models were also applied to estimate all-cause excess deaths “not directly attributable to COVD-19”, i.e., without a previous COVID-19 diagnosis. During the first wave (25th February−31st May), we estimated 52,437 excess deaths (95% CI: 49,213–55,863) and 50,979 (95% CI: 50,333–51,425) during the second phase (10th October−31st December), corresponding to percentage 34.8% (95% CI: 33.8%–35.8%) in the second wave and 31.0% (95%CI: 27.2%–35.4%) in the first. During both waves, all-cause excess deaths percentages were higher in northern regions (59.1% during the first and 42.2% in the second wave), with a significant increase in the rest of Italy (from 6.7% to 27.1%) during the second wave. Males and those aged 80 or over were the most hit groups with an increase in both during the second wave. Excess deaths not directly attributable to COVID-19 decreased during the second phase with respect to the first phase, from 10.8% (95% CI: 9.5%–12.4%) to 7.7% (95% CI: 7.5%–7.9%), respectively. The percentage increase in excess deaths from all causes suggests in Italy a different impact of the SARS-CoV-2 virus during the second wave in 2020. The decrease in excess deaths not directly attributable to COVID-19 may indicate an improvement in the preparedness of the Italian health care services during this second wave, in the detection of COVID-19 diagnoses and/or clinical practice toward the other severe diseases.
“It’s Time To Get Leave,” Says Fetuin-A to Calcium Phosphate
Authors: Jahnen-Dechent Willi , Moshkova Irina
Published: July 2021

Calcium and phosphate are important components of your teeth and bones. Proper incorporation of calcium and phosphate into teeth and bones is called mineralization. If there is too much calcium or phosphate in your blood stream, your body keeps mineralization under control with a protein called fetuin-A. Fetuin-A accompanies calcium and phosphate as they travel through the blood stream, preventing dangerous mineralization from happening in other bodily tissues and helping the excess calcium and phosphate to leave the body through the kidneys.
Exploiting Scanning Surveillance Data to Inform Future Strategies for the Control of Endemic Diseases: The Example of Sheep Scab
Authors: Geddes Eilidh , Mohr Sibylle , Mitchell Elizabeth Sian , Robertson Sara , Brzozowska Anna M. , Burgess Stewart T. G. , Busin Valentina
Published: July 2021

Scanning surveillance facilitates the monitoring of many endemic diseases of livestock in Great Britain, including sheep scab, an ectoparasitic disease of major welfare and economic burden. There is, however, a drive to improve the cost-effectiveness of animal health surveillance, for example by thoroughly exploiting existing data sources. By analysing the Veterinary Investigation Diagnosis Analysis (VIDA) database, this study aimed to enhance the use of existing scanning surveillance data for sheep scab to identify current trends, highlighting geographical “hotspots” for targeted disease control measures, and identifying a denominator to aid the interpretation of the diagnostic count data. Furthermore, this study collated and assessed the impact of past targeted disease control initiatives using a temporal aberration detection algorithm, the Farrington algorithm, to provide an evidence base towards developing cost-effective disease control strategies. A total of 2,401 positive skin scrapes were recorded from 2003 to 2018. A statistically significant decline in the number of positive skin scrapes diagnosed (p < 0.001) occurred across the study period, and significant clustering was observed in Wales, with a maximum of 47 positive scrapes in Ceredigion in 2007. Scheduled ectoparasite tests was also identified as a potential denominator for the interpretation of positive scrapes by stakeholders. Across the study period, 11 national disease control initiatives occurred: four in Wales, three in England, and four in Scotland. The majority (n = 8) offered free diagnostic testing while the remainder involved knowledge transfer either combined with free testing or skills training and the introduction of the Sheep Scab (Scotland) Order 2010. The Farrington algorithm raised 20 alarms of which 11 occurred within a period of free testing in Wales and one following the introduction of the Sheep Scab (Scotland) Order 2010. In summary, our analysis of the VIDA database has greatly enhanced our knowledge of sheep scab in Great Britain, firstly by identifying areas for targeted action and secondly by offering a framework to measure the impact of future disease control initiatives. Importantly this framework could be applied to inform future strategies for the control of other endemic diseases.
Prenatal and Early Postnatal Behavioural Programming in Laying Hens, With Possible Implications for the Development of Injurious Pecking
Authors: De Haas Elske N. , Newberry Ruth C. , Edgar Joanne , Riber Anja B. , Estevez Inma , Ferrante Valentina , Hernandez Carlos E. , Kjaer Joergen B. , Ozkan Sezen , Dimitrov Ivan , Rodenburg T. Bas , Janczak Andrew M.
Published: July 2021

Injurious pecking (IP) represents a serious concern for the welfare of laying hens (Gallus gallus domesticus). The risk of IP among hens with intact beaks in cage-free housing prompts a need for solutions based on an understanding of underlying mechanisms. In this review, we explore how behavioural programmingviaprenatal and early postnatal environmental conditions could influence the development of IP in laying hens. The possible roles of early life adversity and mismatch between early life programming and subsequent environmental conditions are considered. We review the role of maternal stress, egg conditions, incubation settings (temperature, light, sound, odour) and chick brooding conditions on behavioural programming that could be linked to IP. Brain and behavioural development can be programmed by prenatal and postnatal environmental conditions, which if suboptimal could lead to a tendency to develop IP later in life, as we illustrate with a Jenga tower that could fall over if not built solidly. If so, steps taken to optimise the environmental conditions of previous generations and incubation conditions, reduce stress around hatching, and guide the early learning of chicks will aid in prevention of IP in commercial laying hen flocks.
Impact of Packing Density on the Bacterial Community, Fermentation, and In Vitro Digestibility of Whole-Crop Barley Silage
Authors: Sun Lin , Na Na , Li Xiaomei , Li Ziqin , Wang Chao , Wu Xiaoguang , Xiao Yanzi , Yin Guomei , Liu Sibo , Liu Zhiping , Xue Yanlin , Yang Fuyu
Published: July 2021

Packing density has a significant influence on the outcome of ensiling forage. In this study, we aim to investigate the effect of packing density on the ensiling properties, microbiome, and in vitro digestibility of barley silages. Barley was ensiled in polyethylene drum silos (30 L) with respective densities of 600, 650, 700, and 750 kg/m3 fresh matter (FM), and stored for 60 days. The bacterial communities, fermentation quality, and in vitro digestibility were analyzed. Fresh barley had a low count of lactic acid bacteria (LAB, 104 cfu/g of FM), and Lactobacillus was nearly undetectable (<1%). Increasing the packing density decreased the pH and the content of ammonia nitrogen (NH3-N), ethanol, neutral detergent fiber (NDF), and acid detergent fiber (ADF) of barley silage (p < 0.05), and increased in vitro digestibility of dry matter, NDF, ADF, and DM recovery (p < 0.05). A higher packing density decreased the abundances of Enterobacter (from 47.4% to 35.4%) and Clostridium (from 13.5% to 3.8%), and increased the abundance of Lactobacillus (from 1.8% to 17.0%). Thus, packing density positively correlated with Lactobacillus (p < 0.05) but negatively correlated with Enterobacter (p < 0.05). The pH and the content of ethanol were positively correlated with Enterobacter (p < 0.05) but negatively correlated with Lactobacillus (p < 0.05). In conclusion, the density of 750 kg/m3 FM resulted in the highest silage quality of the densities tested.
The Potential of Small Grains Crops in Enhancing Biofortification Breeding Strategies for Human Health Benefit
Authors: Shelenga Tatyana V. , Kerv Yulia A. , Perchuk Irina N. , E. Solovyeva Alla , Khlestkina Elena K. , G. Loskutov Igor , Konarev Alexey V.
Published: July 2021

Nutrition is a source of energy, and building material for the human organism. The quality of food has an effect on the quality of individual life. Minerals and vitamins participate in various catalytic and regulatory functions of the main metabolic processes: absorption, transport, redox and biosynthesis of organic compounds, genetic information transfer, etc. Regular consumption of dietary fibers like β-glucans and oat-specific phenolics, antioxidants, and avenanthramides, stimulate innate and acquired immunity, prevent cancer, obesity, reduce glucose, total cholesterol and triglyceride blood levels and regulate the expression of cholesterol-related genes. Thus, all those compounds are vitally important for the normal functional status of the human body. A deficiency in one or another essential nutrient causes disruptions in human metabolism, thus leading to serious illnesses. Plants are the main source of essential nutrients that are bioavailable for humans. One of the most popular groups of staple crops are the small grains crops (SGC), so these crops are most often used for biofortification purposes. Exploiting the potential of plant resources, biofortification is a long-term strategy, aimed at increasing the number of essential micro- and macronutrients in major food sources and ensuring their bioavailability. The most productive way to implement such strategy is the active use of the possibilities offered by collections of plant genetic resources, including SGC, concentrated in various countries of the world. The collections of plant resources contain both cultivated plants and their wild relatives that possess the required composition of micro- and macronutrients. A complex scientific approach to studying plant germplasm collections, together with agricultural practices (soil enrichment with fertilizers with a required composition), genetic biofortification (traditional breeding, marker-assisted selection or genetic engineering tactics), and their combinations will lead to the development of new biofortified cultivars and improvement of old ones, which can be used to solve the problems of unbalanced nutrition (malnutrition or hidden hunger) in different regions of the world.
Cultivated and Wild Juvenile Thick-Lipped Grey Mullet, Chelon labrosus: A Comparison from a Nutritional Point of View
Authors: García-Márquez Jorge , Galafat Alba , Alarcón Francisco Javier , Figueroa Félix L. , Martínez-Manzanares Eduardo , Arijo Salvador , Abdala-Díaz Roberto Teófilo
Published: July 2021

The thick-lipped grey mullet (Chelon labrosus) is a nominee fish species for aquaculture diversification in Spain because it is an omnivore and euryhaline species, but limited knowledge about the nutritional attributes of this species is available. This study aimed to characterize the chemical composition of wild and cultured fish. The muscle proximate composition, and fatty acid and amino acid profiles were assessed. The cultivated specimens showed a higher lipid content and lower protein and ash contents compared with the wild specimens. The predominant tissue fatty acids in both the wild and cultivated fish were palmitic acid (16:0), oleic acid (18:1n-9) and docosahexaenoic acid (DHA, 22:6n-3). A higher content of arachidonic acid (ARA, 20:4n-6), eicosapentaenoic acid (20:5n-3) and DHA were detected in the muscle of wild mullets, while the fish supplied with commercial pellets showed higher quantities of monounsaturated fatty acids, and lower quantities of saturated fatty acids and polyunsaturated fatty acids (PUFAs). Regarding PUFAs, n-3 fatty acids were predominant in wild mullets, while n-6 and n-9 were more abundant in farmed fish. In terms of amino acid composition, except for histidine in wild specimens, the amino acid amounts were higher than the FAO/WHO standard. In conclusion, C. labrosus may contribute to improving the dietary intake of highly polyunsaturated n-3 fatty acids, with a benefit to human health, owing to that fact that a 100-g fillet portion of cultivated and wild C. labrosus can provide 770 mg and 1160 mg of EPA and DHA, respectively, which exceeds the 250 mg dietary daily intake recommended by the FAO/WHO.
Dietary Supplementation with Saccharomyces cerevisiae, Clostridium butyricum and Their Combination Ameliorate Rumen Fermentation and Growth Performance of Heat-Stressed Goats
Authors: Cai Liyuan , Yu Jiangkun , Hartanto Rudy , Qi Desheng
Published: July 2021

This study aimed to evaluate the effects of Saccharomyces cerevisiae, and their combination on rumen fermentation and growth performance of heat-stressed goats. Twelve heat-stressed goats (20.21 ± 2.30 kg) were divided equally into four groups: control group (CG, fed the basal diet, Saccharomyces cerevisiae supplemented group (SC, 0.60% Saccharomyces cerevisiae added to the basal diet), Clostridium butyricum supplemented group (CB, 0.05% Clostridium butyricum added to the basal diet), and their combination supplemented group (COM 0.60% Saccharomyces cerevisiae and 0.05% Clostridium butyricum added to the basal diet) and were assigned to a 4 × 3 incomplete Latin square design. The rumen fluid and feces were collected for fermentation parameters and feed digestibility analysis, and animal growth performance was also assessed during all the experiment periods. The results showed that rumen pH, rumen cellulolytic enzymes (avicelase, CMCaes, cellobiase, and xylanase) activities, and the concentrations of rumen total volatile fatty acid (TVFA), acetic acid, and propionic acid were significantly increased with Saccharomyces cerevisiae, Clostridium butyricum, and their combination supplementation (p < 0.05). Besides, the dry matter intake (DMI), average daily gain (ADG), and the digestibility of dry matter (DM), neutral detergent fiber (NDF), and acidic detergent fiber (ADF) were significantly increased (p < 0.05) with supplemented these probiotics. However, the ammonia nitrogen (NH3-N) concentration only significantly increased in CB and A/P ratio (acetic acid to propionic acid ratio) only significantly increased in SC and CB. These results indicated that the supplementation with these probiotics could ameliorate rumen fermentation and growth performance of heat-stressed goats.
Differential Scanning Calorimetry as a New Method to Evaluate the Effectiveness of Rejuvenating Agents in Bitumens
Authors: Caputo Paolino , Oliviero Rossi Cesare
Published: July 2021

To date, few methods allow distinguishing a fluxing effect of an additive for bitumen from a regenerating effect. This research aims at identifying a method to accurately establish whether an oxidized bitumen has been regenerated or has simply been fluxed by a softener. Oxidized bitumens, simulating the aging process that results in road pavement lifetime, were prepared by the Rolling thin film oven test (RTFOT) procedure for 225 min and the Pressure Aging Vessel (PAV) procedure. Their asphaltene parts were extracted and analyzed by calorimetry (Differential Scanning Calorimetry DSC), and the results were compared with the presence and absence of a fluxing agent and real rejuvenators. The self-consistent results showed that the thermal properties of the asphaltene fractions is a sound probe to monitor the effect of rejuvenation clearly distinguishable from the mere fluxing effect. This preliminary study might allow the creation of standard protocols capable of identifying a priori the rejuvenating effect of an additive in the future. Furthermore, given the widespread use of calorimetry for the characterization, it tends to become a widely accessible and useful tool for this purpose in material characterization laboratories.
Integration of DE Algorithm with PDC-APF for Enhancement of Contour Path Planning of a Universal Robot
Authors: Kazim Issraa Jwad , Tan Yuegang , Qaseer Layth
Published: July 2021

In the robotic engineering field, the main target, especially in industry, manufacturing, and surgical operations, is reaching the optimal performance of manipulators. The purpose of this paper is to quantify the contour tracking performance of collaborative universal manipulator robot (UR5) by setting the gain of position domain controller. In order to improve and enhance the track of manipulator in experimental applications we utilize differential evolution (DE) optimization, using MATLAB toolbox with an applied robot operating system (ROS). The adopted current approach does not only optimize the gain of position domain controller but also prevent collisions by detecting a “border crossing” without turning off the manipulator and allowing the automation agent to be on the scene, coexisting in harmonic mode and avoiding collisions. This requires the implementation of an algorithm that detects an obstacle to avoid anticipated collisions. For this purpose, the adopted algorithm uses the DE algorithm to modify the artificial potential field (APF). The results of this paper present that on one hand, meta-heuristic optimization algorithm features give the best performance indices for linear and non-linear contours, and on the other hand, DE algorithm features give good modification to APF to generate collision free contour path planning.
Detection of Respiratory Phases in a Breath Sound and Their Subsequent Utilization in a Diagnosis
Authors: Skalicky David , Koucky Vaclav , Hadraba Daniel , Viteznik Martin , Dub Martin , Lopot Frantisek
Published: July 2021

Detection of lung sounds and their propagation is a powerful tool for analysing the behaviour of the respiratory system. A common approach to detect the respiratory sounds is lung auscultation, however, this method has significant limitations including low sensitivity of human ear or ambient background noise. This article targets the major limitations of lung auscultation and presents a new approach to analyse the respiratory sounds and visualise them together with the respiratory phases. The respiratory sounds from 41 patients were recorded and filtered to eliminate the ambient noise and noise artefacts. The filtered signal is processed to identify the respiratory phases. The article also contains an approach for removing the noise that is very difficult to filter but the removal is crucial for identifying the respiratory phases. Finally, the respiratory phases are overlaid with the frequency spectrum which simplifies the orientation in the recording and additionally offers the information on the inter-individual ratio of the inhalation and exhalation phases. Such interpretation provides a powerful tool for further analysis of lung sounds, simplifythe diagnosis of various types of respiratory tract dysfunctions, and returns data which are comparable among the patients.
Experimental Study of Explosion Mitigation by Deployed Metal Combined with Water Curtain
Authors: Schunck Thérèse , Eckenfels Dominique
Published: July 2021

In this paper, protective barriers made of perforated plates with or without a water cover were investigated. In urban areas, such barriers could be envisaged for the protection of facades. An explosive-driven shock tube, combined with a retroreflective shadowgraph technique, was used to visualize the interaction of a blast wave profile with one or two plates made of expanded metal. Free-field air blast experiments were performed in order to evaluate the solution under real conditions. Configurations with either one or two grids were investigated. The transmitted pressure was measured on a wall placed behind the plate(s). It was observed that the overpressure and the impulse downstream of the plate(s) were reduced and that the mitigation performance increased with the number of plates. Adding a water layer on one grid contributed to enhance its mitigation capacity. In the setup with two plates, the addition of a water cover on the first grid induced only a modest improvement. This blast mitigation solution seems interesting for protection purposes.
Deep Morphological Anomaly Detection Based on Angular Margin Loss
Authors: Kim Taehyeon , Hong Eungi , Choe Yoonsik
Published: July 2021

Deep anomaly detection aims to identify “abnormal” data by utilizing a deep neural network trained on a normal training dataset. In general, industrial visual anomaly detection systems distinguish between normal and “abnormal” data through small morphological differences such as cracks and stains. Nevertheless, most existing algorithms emphasize capturing the semantic features of normal data rather than the morphological features. Therefore, they yield poor performance on real-world visual inspection, although they show their superiority in simulations with representative image classification datasets. To address this limitation, we propose a novel deep anomaly detection algorithm based on the salient morphological features of normal data. The main idea behind the proposed algorithm is to train a multiclass model to classify hundreds of morphological transformation cases applied to all the given data. To this end, the proposed algorithm utilizes a self-supervised learning strategy, making unsupervised learning straightforward. Additionally, to enhance the performance of the proposed algorithm, we replaced the cross-entropy-based loss function with the angular margin loss function. It is experimentally demonstrated that the proposed algorithm outperforms several recent anomaly detection methodologies in various datasets.
Design of an Intelligent Variable-Flow Recirculating Aquaculture System Based on Machine Learning Methods
Authors: Chen Fudi , Du Yishuai , Qiu Tianlong , Xu Zhe , Zhou Li , Xu Jianping , Sun Ming , Li Ye , Sun Jianming
Published: July 2021

A recirculating aquaculture system (RAS) can reduce water and land requirements for intensive aquaculture production. However, a traditional RAS uses a fixed circulation flow rate for water treatment. In general, the water in an RAS is highly turbid only when the animals are fed and when they excrete. Therefore, RAS water quality regulation technology based on process control is proposed in this paper. The intelligent variable-flow RAS was designed based on the circulating pump-drum filter linkage working model. Machine learning methods were introduced to develop the intelligent regulation model to maintain a clean and stable water environment. Results showed that the long short-term memory network performed with the highest accuracy (training set 100%, test set 96.84%) and F1-score (training 100%, test 93.83%) among artificial neural networks. Optimization methods including grid search, cuckoo search, linear squares, and gene algorithm were proposed to improve the classification ability of support vector machine models. Results showed that all support vector machine models passed cross-validation and could meet accuracy standards. In summary, the gene algorithm support vector machine model (accuracy: training 100%, test 98.95%; F1-score: training 100%, test 99.17%) is suitable as an optimal variable-flow regulation model for an intelligent variable-flow RAS.
Low Power Cusped Field Thruster Developed for the Space-Borne Gravitational Wave Detection Mission in China
Authors: Liu Hui , Zeng Ming , Niu Xiang , Huang Hongyan , Yu Daren
Published: July 2021

The microthruster is the crucial device of the drag-free attitude control system, essential for the space-borne gravitational wave detection mission. The cusped field thruster (also called the High Efficiency Multistage Plasma Thruster) becomes one of the candidate thrusters for the mission due to its low complexity and potential long life over a wide range of thrust. However, the prescribed minimum of thrust and thrust noise are considerable obstacles to downscaling works on cusped field thrusters. This article reviews the development of the low power cusped field thruster at the Harbin Institute of Technology since 2012, including the design of prototypes, experimental investigations and simulation studies. Progress has been made on the downscaling of cusped field thrusters, and a new concept of microwave discharge cusped field thruster has been introduced.
The Team Handball Game-Based Performance Test Is Better than the Yo-Yo Intermittent Recovery Test to Measure Match-Related Activities in Female Adult Top-Elite Field Team Handball Players
Authors: Michalsik Lars Bojsen , Fuchs Patrick , Wagner Herbert
Published: July 2021

In team handball, suitable tests determining the match-related physical performance are essential for the planning of optimal physical training regimens. Thus, the aims of the present study were (a) to determine the relationships between the physical and physiological test results from a team handball game-based performance test (GBPT), the Yo-Yo intermittent recovery test, level 1 (Yo-Yo IR1 test) and a separate linear 30-m single sprint performance test (SSPT) in female adult top-elite field team handball players, in order to establish the significance (validity) of tests for measuring relevant elements for team handball match-play; and (b) to compare and evaluate the results from the aforementioned tests for the same players in relation to the different playing positions. Twenty-three female adult top-elite field team handball players from the Danish Premier Female Team Handball League performed the GBPT, the Yo-Yo IR1 test and the 30-m SSPT test on separate days. As main findings, significant correlations between the GBPT and the Yo-Yo IR1 test in about 1/3 of the variables were found, indicating that the Yo-Yo IR1 test is reflecting most of the locomotive match activities in female adult top-elite team handball. However, the Yo-Yo IR1 test results were not correlated to the GBPT in any of the match-related activities in the team handball GBPT that included technical playing actions such as tackles, passes, jumping and shooting during specialized movements in offence and defence. Overall, the results revealed that the GBPT is better than the Yo-Yo IR1 test to evaluate female adult top-elite field team handball players’ ability to perform physical match-related activities including both locomotive and technical playing actions executed as during competitive match-play. Similar to the Yo-Yo IR1 test, the 30-m SSPT was not correlated to any of the team handball GBPT activities, which included technical playing actions. These data suggest that the SSPT only to a certain extent can measure the individual sprint capacity of elite team handball players. In addition, unexpectedly no significant differences between the various playing positions were found neither for the GBPT, the SSPT nor the Yo-Yo IR1 test. However, several effects sizes indicated that the lack of positional differences primarily was due to the relatively small sample size in each playing position and the composition of the specific group of players. In conclusion, this study clearly indicated that team handball specific physical performance, as measured by the GBPT, and general physical performance, as measured by the Yo-Yo IR1 test and the 30-m SSPT, are different components. This must be taken in consideration when using physical test results for the planning of optimal physical training regimens in elite team handball.