In-depth analysis of the treatment effect and synergistic mechanism of TanReQing injection on clinical multi-drug resistant
Pseudomonas aeruginosa
Authors: Li Dongying , Li Yueyi , Wang Jingyi , Yang Weifeng , Cui Kaiyu , Su Renjing , Li Lu , Ren Xing , Li Xianyu , Wang Yi 
Published: February 2024

Antibiotic resistance is a recognized and concerning public health issue. Gram-negative bacilli, such as
Pseudomonas aeruginosa
(
P. aeruginosa
), are notorious for their rapid development of drug resistance, leading to treatment failures. TanReQing injection (TRQ) was chosen to explore its pharmacological mechanisms against clinical multidrug-resistant
P. aeruginosa
(MDR-PA), given its antibacterial and anti-inflammatory properties. We revealed the expression of proteins and genes in
P. aeruginosa
after co-culture with TRQ. This study developed an assessment method to evaluate clinical resistance of
P. aeruginosa
using MALDI-TOF MS identification and Biotyper database searching techniques. Additionally, it combined MIC determination to investigate changes in MDR-PA treated by TRQ. TRQ effectively reduced the MICs of ceftazidime and cefoperazone and enhanced the confidence scores of MDR-PA as identified by mass spectrometry. Using this evaluation method, the fingerprints of standard
P. aeruginosa
and MDR-PA were compared, and the characteristic peptide sequence (Seq-PA No. 1) associated with flagellum was found. The phenotypic experiments were conducted to confirm the effect of TRQ on the motility and adhesion of
P. aeruginosa
. A combination of co-immunoprecipitation and proteome analysis was employed, and 16 proteins were significantly differentially expressed and identified as potential candidates for investigating the mechanism of inhibiting resistance in
P. aeruginosa
treated by TRQ. The candidates were verified by quantitative real-time PCR analysis, and TRQ may affect these core proteins (MexA, MexB, OprM, OprF, OTCase, IDH, and ASL) that influence resistance of
P. aeruginosa
. The combination of multiple methods helps elucidate the synergistic mechanism of TRQ in overcoming resistance of
P. aeruginosa
.

IMPORTANCE

Pseudomonas aeruginosa
is an opportunistic pathogen closely associated with various life-threatening acute and chronic infections. The presence of antimicrobial resistance and multidrug resistance in
P. aeruginosa
infections significantly complicates antibiotic treatment. The expression of β-lactamase, efflux systems such as MexAB-OprM, and outer membrane permeability are considered to have the greatest impact on the sensitivity of
P. aeruginosa
. The study used a method to assess the clinical resistance of
P. aeruginosa
using matrix-assisted laser desorption ionization time of flight mass spectrometry identification and Biotyper database search techniques. TanReQing injection (TRQ) effectively reduced the MICs of ceftazidime and cefoperazone in multidrug-resistant
P. aeruginosa
(MDR-PA) and improved the confidence scores for co-cultured MDR-PA. The study found a characteristic peptide sequence for distinguishing whether
P. aeruginosa
is resistant. Through co-immunoprecipitation and proteome analysis, we explored the mechanism of TRQ overcoming resistance of
P. aeruginosa
.