bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-, Inter-, and Transdisciplinarity within the Public Health Workforce: A Scoping Review to Assess Definitions and Applications of Concepts
Authors: Sell Kerstin , Hommes Franziska , Fischer Florian , Arnold Laura
Published: September 2022

In light of the current public health challenges, calls for more inter- and transdisciplinarity in the public health workforce are increasing, particularly to respond to complex and intersecting health challenges, such as those presented by the climate crisis, emerging infectious diseases, or military conflict. Although widely used, it is unclear how the concepts of multi-, inter-, and transdisciplinarity are applied with respect to the public health workforce. We conducted a scoping review and qualitative content analysis to provide an overview of how the concepts of multi-, inter-, and transdisciplinarity are defined and applied in the academic literature about the public health workforce. Of the 1957 records identified, 324 articles were included in the review. Of those, 193, 176, and 53 mentioned the concepts of multi-, inter-, and transdisciplinarity, respectively. Overall, 44 articles provided a definition. Whilst definitions of multidisciplinarity were scarce, definitions of inter- and transdisciplinarity were more common and richer, highlighting the aim of the collaboration and the blurring and dissolution of disciplinary boundaries. A better understanding of the application of multi-, inter-, and transdisciplinarity is an important step to implementing these concepts in practice, including in institutional structures, academic curricula, and approaches in tackling public health challenges.
Consistency Analysis in Medical Empathy Intervention Research
Authors: Lee Meng-Lin , Hsieh Ton-Lin , Yang Chih-Wei , Chen Jou-Chieh , Ju Yu-Jeng , Hsueh I-Ping
Published: September 2022

Various studies have examined the effectiveness of interventions to increase empathy in medical professionals. However, inconsistencies may exist in the definitions, interventions, and assessments of empathy. Inconsistencies jeopardize the internal validity and generalization of the research findings. The main purpose of this study was to examine the internal consistency among the definitions, interventions, and assessments of empathy in medical empathy intervention studies. We also examined the interventions and assessments in terms of the knowledge–attitude–behavior aspects. We conducted a literature search for medical empathy intervention studies with a design of randomized controlled trials and categorized each study according to the dimensions of empathy and knowledge–attitude–behavior aspects. The consistencies among the definitions, interventions, and assessments were calculated. A total of 13 studies were included in this study. No studies were fully consistent in their definitions, interventions, and assessments of empathy. Only four studies were partially consistent. In terms of knowledge–attitude–behavior aspects, four studies were fully consistent, two studies were partially consistent, and seven studies were inconsistent. Most medical empathy intervention studies are inconsistent in their definitions, interventions, and assessments of empathy, as well as the knowledge–attitude–behavior aspects between interventions and assessments. These inconsistencies may have affected the internal validity and generalization of the research results.
Can the Participation in Quality Certification of Agricultural Products Drive the Green Production Transition?
Authors: Li Xiaojing , Xia Xianli , Ren Jiazhen
Published: September 2022

The green production transition in agriculture is all about the quality of agricultural products at the source of production. Whether the product quality certification can accelerate the green production transition in agriculture is an issue of concern. We have measured the degree of green production transition of kiwifruit growers using a finite mixture model in this paper, and use research data from the main kiwifruit production areas in Shaanxi and Sichuan provinces to verify the impact of conducting product quality certification on the green production transition of kiwifruit growers. Besides, we use a multi-valued treatment effects model to verify the differences in the degree of green production transition among kiwifruit growers in the face of different certification types. Our findings are mainly as follows: the degree of green production transition among kiwifruit growers is not high, with an average of only 36.3%. Product quality certification can significantly promote the green production transition of kiwifruit growers, and the promotion effect of different certification methods in green production transition of kiwifruit growers significantly varies. The promotion effect of organic certification is greater than that of green certification and pollution-free certification. Further, the mechanism test analysis reveals that product quality certification can influence the green production transition of kiwifruit growers through three mechanisms: quality monitoring, market premium, and market access threshold. Based on this, this paper proposes policy recommendations to advance quality certification and green production transition among kiwifruit growers to increase the certification, enhance the willingness to green transition, and boost the differentiated certification system.
Promotion of Food and Beverages by German-Speaking Influencers Popular with Adolescents on TikTok, YouTube and Instagram
Authors: Winzer Eva , Naderer Brigitte , Klein Simeon , Lercher Leah , Wakolbinger Maria
Published: September 2022

The promotion of nutritionally poor food and beverages (F&B) has a proven effect on children’s eating preferences and, therefore, plays a significant role in today’s childhood obesity epidemic. This study’s objective was to assess the prevalence (exposure) and context (power) of the F&B cues in influencer content across three platforms: TikTok, YouTube, and Instagram. The selected influencers were popular with adolescents, with a combined total of more than 34 million followers/subscribers. We employed the YouTube Influencer Marketing Protocol from the World Health Organization (WHO) as our basis for coding. We analysed a total of 360 videos/posts and, of these, 24% contained F&B cues, which is equivalent to 18.1 F&B cues/hour. In total, 77% of the cues were not permitted for children’s advertising, according to WHO criteria, and this was stable across all platforms, with chocolate and sugary confectionery (23%) as the most frequently featured products. Not-permitted F&B had a four-times higher chance of being branded, a five-times higher chance of being described positively, and received significantly more ‘likes’. In 62% of the analysed presentations, the branded product was mentioned, yet only 6% of the content was labelled as advertising. The present analysis delivers further grounds for discussion for policies and regulations of influencer marketing.
The Importance of Local and Global Social Ties for the Mental Health and Well-Being of Recently Resettled Refugee-Background Women in Australia
Authors: Murray Kate E. , Lenette Caroline , Brough Mark , Reid Katherine , Correa-Velez Ignacio , Vromans Lyn , Schweitzer Robert D.
Published: September 2022

Social connections are foundational to the human condition and are inherently disrupted when people are forcibly displaced from their home countries. At a time of record high global forced migration, there is value in better understanding how refugee-background individuals engage theirsocial supports or ties in resettlement contexts. A mixed methods research design aimed to understand the complexities of how 104 refugee-background women experienced their social networks in the first few months of resettlement in Australia. One of the research activities involved participants completing a survey with both quantitative and qualitative components. The quantitative analyses identified the impact of post-migration living difficulties that represented social stressors (worry about family, loneliness and boredom, feeling isolated, and racial discrimination) on the women’s mental health outcomes in the months following resettlement. The qualitative data highlighted the complexities of social relationships serving as both stressors and sources of support, and the importance of recognizing extended families and supports around the globe. The findings point to the need for nuanced accounts of the social contexts surrounding refugee resettlement as important influences able to promote trauma-informed and gender sensitive practices to support mental health and well-being in new settings.
Exploring the Impact of Digital Inclusive Finance on Agricultural Carbon Emission Performance in China
Authors: Sun Le , Zhu Congmou , Yuan Shaofeng , Yang Lixia , He Shan , Li Wuyan
Published: September 2022

This paper attempts to reveal the impact and mechanisms of digital inclusive finance (DIF) on agricultural carbon emission performance (ACEP). Specifically, based on the provincial panel data in China from 2011 to 2020, a super slacks-based measure (Super SBM) model is applied to measure ACEP. The panel regression model and spatial regression model are used to empirically analyze the impact of DIF on ACEP and its mechanism. The results show that: (1) during the study period, China’s ACEP exhibited a continuous growth trend, and began to accelerate after 2017. The high-value agglomeration areas of ACEP shifted from the Huang-Huai-Hai plain and the Pearl River Delta to the coastal regions and the Yellow River basin, the provincial differences displayed an increasing trend from 2011 to 2020. (2) DIF was found to have a significant positive impact on ACEP. The main manifestation is that the development of the coverage breadth and depth of use of DIF helps to improve the ACEP. (3) The positive impact of DIF on ACEP had a significant spatial spillover effect, that is, it had a positive effect on the improvement of ACEP in the surrounding provinces. These empirical results can help policymakers better understand the contribution of DIF to low-carbon agriculture, and provide them with valuable information for the formulation of supportive policies.
Effect of Eyeglasses on Student Academic Performance: What Matters? Evidence from a Randomized Controlled Trial in China
Authors: Du Kang , Wang Huan , Ma Yue , Guan Hongyu , Rozelle Scott
Published: September 2022

Although eyeglasses have been considered a cost-effective way to combat myopia, the empirical evidence of its impacts on improving learning outcomes is inconsistent. This paper provides empirical evidence examining the effect of providing eyeglasses on academic performance between provinces with a different economic level in western China. Overall, we find a significant impact in Intention-to-Treat analysis and a large and significant local average treatment effect of providing free eyeglasses to students in the poor province but not in the other. The difference in impact between the two provinces is not a matter of experimental design, implementation, or partial compliance. Instead, we find that the lack of impact in the wealthier provinces is mainly due to less blackboard usage in class and wealthier households. Our study found that providing free eyeglasses to disadvantaged groups boosted their academic performance more than to their counterparts.
Nurses Coping with Stressful Situations—A Cross-Sectional Study
Authors: Iwanowicz-Palus Grażyna , Mróz Mariola , Kowalczuk Krystyna , Szlendak Beata , Bień Agnieszka , Cybulski Mateusz
Published: September 2022

Nursing belongs to the group of professions particularly exposed to stress. Since the ability to cope with stress is an important aspect of mental health, the aim of this study was to identify the types of nurses’ behaviours in terms of different coping styles used when dealing with work-related and psychosocial stress. The study was conducted among 1223 Polish nurses by means of a diagnostic survey, using the Coping in Stressful Situations Questionnaire (CISS), the Generalised Self-Efficacy Scale (GSES) and a questionnaire of the author’s own design. Three types of nurses were distinguished: Type 1 (non-harmonious/organised)—nurses with lower professional education, longer work experience, at least average severity of stress related to working conditions, the lowest GSES scores, and worse psychophysical condition, who focused on their own emotional state when faced with stressful situations. Type 2 (harmonious)—nurses with higher education, the lowest intensity of work-related stresses, the highest GSES scores, positive self-reported psycho-physical condition, most often using the task-oriented coping style in stressful situations. Type 3 (non-harmonious/disorganised)—nurses with shorter length of service in the profession, the highest intensity of work-related stress, average GSES scores, and poorer self-reported psycho-physical condition. The presented results may provide a basis for preventive measures to minimise stress and increase competence in coping strategies, thus contributing to improved psychological and physical well-being of nurses.
Psychological and Biochemical Effects of an Online Pilates Intervention in Pregnant Women during COVID-19: A Randomized Pilot Study
Authors: Kim Hyun-Bin , Hyun Ah-Hyun
Published: September 2022

The purpose of this study was to analyze the effect of real-time online Pilates exercise during COVID-19 on women’s body composition, blood lipids, and psychological health after childbirth. The participants were 16 pregnant women (24–28 weeks pregnant) enrolled at the C Women’s Culture Center in Seoul, South Korea, classified into online Pilates groups and non-exercise groups (PE, n = 8; CON, n = 8). The online Pilates program was conducted for 8 weeks, twice a week, and 50 min a day using a real-time video chat app. Participants visited the hospital twice for body composition and blood tests. Questionnaires on postpartum depression, sleep disorder, and stress were conducted at 6 weeks and 12 weeks after childbirth. We found a significant difference between groups in body composition. The weight, percentage of body fat, body fat mass, and BMI of the PE group decreased. Blood lipids showed significant differences between the groups in TC, TG, LDL and CRP, while insulin and HDL showed no difference. All blood lipids, insulin, and CRP in the PE group were reduced. There were significant differences between the groups in postpartum depression, sleep disorders, and perceived stress indices performed in the post-test, and the serotonin concentration in the PE group increased. Serotonin levels were significantly correlated with postpartum depression, body fat mass, and body fat rate. Pregnant women’s online Pilates in this study was effective at reducing weight and depression in women after childbirth and should be used to promote women’s mental health during COVID-19.
How Work Stress Impacts Emotional Outcomes of Chinese College Teachers: The Moderated Mediating Effect of Stress Mindset and Resilience
Authors: Yu Tao , Li Jiayuan , He Lidong , Pan Xiaofu
Published: September 2022

Based on the job demands-resources model and conservation of resource theory, this study investigated 456 Chinese college teachers’ work stress, stress mindset, resilience, emotional exhaustion, positive affect, and negative affect. The results of mediation analysis showed that resilience played a partial mediation role between work stress and emotional outcomes (emotional exhaustion, positive affect, and negative affect). Moreover, the results of a moderated mediation analysis showed that stress mindset moderated the relationship between work stress and resilience, and moderated the mediating effect of resilience between work stress and emotional outcomes (emotional exhaustion, positive affect, and negative affect). Specifically, work stress had a significant negative predictive effect on resilience when stress mindset is low (β = −0.54, p < 0.001); work stress could also negatively predict resilience when the stress mindset is high (β = −0.47, p < 0.001), but its effect decreased, and stress mindset negatively moderated the path between work stress and resilience. Finally, we discussed theoretical implications, practical implications, limitations, and future directions.
Acute Effect of Exposure to Extreme Heat (100 ± 3 °C) on Lower Limb Maximal Resistance Strength
Authors: Bartolomé Ignacio , Toro-Román Víctor , Siquier-Coll Jesús , Muñoz Diego , Robles-Gil María , Maynar-Mariño Marcos
Published: September 2022

The aim of this study was to evaluate the acute effect of a single dry sauna bath lasting twelve minutes on the indirect determination of the one maximum repetition (1RM) leg press among trained and untrained participants. Thirty young men participated in the study, a trained group (TG; n = 15; age: 20.97 ± 0.44 years) and an untrained group (UG; n = 15; age: 21.03 ± 0.11 years). Subjects in the TG had performed resistance training for at least two years before the beginning of the experiment. All participants performed two indirect tests of their one maximum repetition leg press on two different days, with a rest period of three weeks between tests. Additionally, anthropometric, body composition, blood pressure, body temperature, and rated perceived exertion were evaluated. On the second testing day, all of the participants took a dry sauna bath lasting 12 min immediately before performing the leg press test. In the second evaluation (pre-heating in the sauna), the UG experienced increases in absolute RM (178.48 ± 56.66 to 217.60 ± 59.18 kg; p < 0.05; R = 0.798), relative RM (2.65 ± 0.61 to 3.24 ± 0.58 kg·g body mass−1; p < 0.05; R = 0.798), and muscular RM (5.64 ± 1.20 to 6.77 ± 1.14 kg·kg muscle mass−1; p < 0.05; R = 0.797). The TG also increased their values on the second day in absolute RM (284.96 ± 62.41 to 314.92 ± 1.04 kg; p < 0.01; R = 0.886), in relative RM (3.61 ± 0.88 to 3.99 ± 1.85 kg*kg body mass−1; p < 0.01; R = 0.886), and muscular RM (7.83 ± 1.69 to 8.69 ± 1.85 kg·kg muscle mass−1; p < 0.01; R = 0.854). A passive, extreme-heat sauna bath lasting 12 min taken immediately before a relative maximum repetition test seems to provoke clear positive responses for the development of strength.
Work from Home during the COVID-19 Pandemic—The Impact on Employees’ Self-Assessed Job Performance
Authors: Kifor Claudiu Vasile , Săvescu Roxana Florența , Dănuț Raluca
Published: September 2022

This study investigates the impact of remote workplace factors on employees’ social and technical self-assessed performance during the COVID-19 pandemic. The impact of the variables belonging to the employee’s profile, organizational environment, and work-life balance categories on social and technical performance were analyzed, based on a survey of 801 Romanian employees, using ordinary least squares and quantile regression techniques. While the first method provided summary point estimates that calculated the average effect of the explanatory variables for the “average employee”, the second approach allowed us to focus on the effects explanatory variables have on the entire conditional distribution of the response variables, taking into account that this effect can be different for employees with different levels of performance. Job autonomy, engagement, communication skills, trust in co-workers, occupational self-efficacy, and family-work conflict, significantly influence both social and technical performance. PhD education and trust in management significantly influence social performance, while motivation, stress, the share of time spent in remote work, organizational commitment, children in the household, and household size, influence only technical performance.
Contribution of Fine Roots to Soil Organic Carbon Accumulation in Different Desert Communities in the Sangong River Basin
Authors: Tian Sihui , Liu Xin , Jin Baocheng , Zhao Xuechun
Published: September 2022

This study explored the relationship between soil organic carbon (SOC) and root distribution, with the aim of evaluating the carbon stocks and sequestration potential under five plant communities (Alhagi sparsifolia, Tamarix ramosissima, Reaumuria soongorica, Haloxylon ammodendron, and Phragmites communis) in an arid region, the Sangong River watershed desert ecosystem. Root biomass, ecological factors, and SOC in different layers of a 0–100 cm soil profile were investigated. The results demonstrated that almost all living fine root biomass (11.78–34.41 g/m2) and dead fine root biomass (5.64–15.45 g/m2) levels were highest in the 10–20 cm layer, except for the P. communis community, which showed the highest living and dead fine root biomass at a depth of 60–70 cm. Fine root biomass showed strong seasonal dynamics in the five communities from June to October. The biomass levels of the A. sparsifolia (138.31 g/m2) and H. ammodendron (229.73 g/m2) communities were highest in August, whereas those of the T. ramosissima (87.76 g/m2), R. soongorica (66.29 g/m2), and P. communis (148.31 g/m2) communities were highest in September. The SOC of the five communities displayed strong changes with increasing soil depth. The mean SOC value across all five communities was 77.36% at 0–30 cm. The highest SOC values of the A. sparsifolia (3.08 g/kg), T. ramosissima (2.35 g/kg), and R. soongorica (2.34 g/kg) communities were found in June, and the highest value of the H. ammodendron (2.25 and 2.31 g/kg, p > 0.05) community was found in June and September. The highest SOC values of the P. communis (1.88 g/kg) community were found in July. Fine root production and turnover rate were 50.67–486.92 g/m2/year and 1.25–1.98 times per year. The relationships among SOC, fine root biomass, and ecological factors (soil water content and soil bulk density) were significant for all five communities. Based on the results, higher soil water content and soil conductivity favored the decomposition of root litter and increased fine root turnover, thereby facilitating SOC formation. Higher pH and bulk density levels are not conducive to soil biological activity and SOC mineralization, leading to increased SOC levels in desert regions.
Use of Telemedicine Technology among General Practitioners during COVID-19: A Modified Technology Acceptance Model Study in Poland
Authors: Walczak Renata , Kludacz-Alessandri Magdalena , Hawrysz Liliana
Published: September 2022

During the COVID-19 pandemic, telehealth became a popular solution for the remote provision of primary care by General Practitioners (GPs) in Poland. This study aimed to assess the GPs’ acceptance of telehealth during the COVID-19 pandemic in Poland and to explain the factors that drive GPs’ need to implement a telehealth system in primary care using the modified Technology Acceptance Model (TAM). In Poland, 361 GPs from a representative sample of 361 clinics drawn from 21,500 outpatient institutions in Poland participated in the empirical study. Structural equation modelling (SEM) was used to evaluate the causal relationships that were formulated in the proposed model. Research has shown that Polish GPs reported a positive perception and high acceptance of the telehealth system during the COVID-19 pandemic. Overall, the results show that the social factors (image, decision autonomy, perception of patient interaction) significantly positively influence the technological factors (perceived ease of use and perceived usefulness) that influence the need to implement a telehealth system. The proposed socio-technological model can serve as a theoretical basis for future research and offer empirical predictions for practitioners and researchers in health departments, governments, and primary care settings.
Effect of Seed Sludge Type on Aerobic Granulation, Pollutant Removal and Microbial Community in a Sequencing Batch Reactor Treating Real Textile Wastewater
Authors: Zou Jinte , Yang Jiaqi , He Hangtian , Wang Xiaofei , Mei Rongwu , Cai Lei , Li Jun
Published: September 2022

The aerobic granulation, pollutant removal, and microbial community in real textile wastewater (TWW) treatment were compared using conventional activated sludge (CAS) and preformed aerobic granular sludge (AGS) in synthetic wastewater as seed in two reactors, reactor-1 (R1) and reactor-2 (R2), respectively. The results showed that complete granulation was achieved in R1 (sludge volume index at 5 min (SVI5) and 30 min (SVI30): 19.4 mL/g; granule size: 210 μm) within 65 days, while it only required 28 days in R2 (SVI5 and SVI30: 27.3 mL/g; granule size: 496 μm). The removal of COD, NH4+-N and TN in R1 (49.8%, 98.8%, and 41.6%) and R2 (53.6%, 96.9%, and 40.8%) were comparable in 100% real TWW treatment, but stable performance was achieved much faster in R2. The real TWW had an inhibitory effect on heterotrophic bacteria activity, but it had no inhibition on ammonia-oxidizing bacteria activity. AGS with a larger particle size had a higher microbial tolerance to real TWW. Furthermore, filamentous Thiothrix in the AGS in R2 disappeared when treating real TWW, leading to the improvement of sludge settleability. Thus, seeding preformed AGS is suggested as a rapid start-up method for a robust AGS system in treating real TWW.
The Relationship between Healthcare Providers and Preventive Practices: Narratives on Access to Cancer Screening
Authors: Lemmo Daniela , Martino Maria Luisa , Donizzetti Anna Rosa , Freda Maria Francesca , Caso Daniela
Published: September 2022

Cancer screening programs are public health interventions beneficial to early diagnoses and timely treatments. Despite the investment of health policies in this area, many people in the recommended age groups do not participate. While the literature is mainly focused on obstacles and factors enabling access to health services, a gap from the point of view of the target population concerns healthcare providers. Within the “Miriade” research–action project, this study aims to explore the dimensions that mediate the relationship between healthcare providers and preventive practices through the narrations of 52 referents and healthcare providers involved in breast, cervical and colorectal cancer screening. We conducted ad hoc narrative interviews and used theory-driven analysis based on Penchansky and Thomas’ conceptualization and Saurman’s integration of six dimensions of healthcare access: affordability, availability, accessibility, accommodation, acceptability and awareness. The results show that 21 thematic categories were representative of the access dimensions, and 5 thematic categories were not; thus, we have classified the latter as the dimension of affection. The results suggest trajectories through which psychological clinical intervention might be constructed concerning health, shared health decisions and access to cancer screening.
Urban Environment, Green Urban Areas, and Life Quality of Citizens—The Case of Warsaw
Authors: Stangierska Dagmara , Kowalczuk Iwona , Juszczak-Szelągowska Ksenia , Widera Katarzyna , Ferenc Weronika
Published: September 2022

The increased migration of people from rural areas to cities has prompted researchers to take an interest in the problem of the quality of life (QOL) of the urban population in different contexts. The aim of the study was to determine the relationship between the level of satisfaction of Warsaw residents with urban infrastructure (SUI) and their QOL, the impact of the SUI on the perception of a neighborhood as an ideal place to live and the relationship between the amount of green areas and and the SUI of Warsaw residents and their QOL. The quantitative survey was conducted using the CAWI method on a sample of 381 adults. The WHOQOL-BREF questionnaire was used to measure QOL, the scale used in earlier surveys was used to assess SUI, areas of of Warsaw with different amounts of green space were distinguished using cluster analysis. The study showed a relationship between the SUI declared by residents and their QOL, mainly in the environmental domain. The discriminant analysis showed that satisfaction with greenery is one of the most important determinants of the subjective perception of a neighborhood as an ideal place to live. There was no direct effect of the amount of green areas in objective terms on the QOL of Warsawians, but a relationship was noted between the amount of green areas and SUI, with the highest level of satisfaction noted for the Green-balanced Cluster, characterized by the most favorable combination of quality and utility of urban area.
Elevated Oxytocin Receptor Blood Concentrations Predict Higher Risk for, More, and Earlier 24-Month Hospital Readmissions after In-Patient Detoxification in Males with Alcohol Use Disorder
Authors: Mühle Christiane , Mazza Massimiliano , Weinland Christian , von Zimmermann Claudia , Bach Patrick , Kiefer Falk , Grinevich Valery , Zoicas Iulia , Kornhuber Johannes , Lenz Bernd
Published: September 2022

Alcohol use disorder (AUD) is a major global mental health challenge. Knowledge concerning mechanisms underlying AUD and predictive biomarkers of AUD progression and relapse are insufficient. Recently, addiction research is focusing attention on the oxytocin system. However, to our knowledge, blood concentrations of the oxytocin receptor (OXTR) have not yet been studied in AUD. Here, in sex-separated analyses, OXTR serum concentrations were compared between early-abstinent in-patients with AUD (113 men, 87 women) and age-matched healthy controls (133 men, 107 women). The OXTR concentrations were correlated with sex hormone and oxytocin concentrations and alcohol-related hospital readmissions during a 24-month follow-up. In male patients with AUD, higher OXTR concentrations were found in those with an alcohol-related readmission than in those without (143%; p = 0.004), and they correlated with more prospective readmissions (ρ = 0.249; p = 0.008) and fewer days to the first readmission (ρ = −0.268; p = 0.004). In men and women, OXTR concentrations did not significantly differ between patients with AUD and controls. We found lower OXTR concentrations in smokers versus non-smokers in female patients (61%; p = 0.001) and controls (51%; p = 0.003). In controls, OXTR concentrations correlated with dihydrotestosterone (men, ρ = 0.189; p = 0.030) and testosterone concentrations (women, ρ = 0.281; p = 0.003). This clinical study provides novel insight into the role of serum OXTR levels in AUD. Future studies are encouraged to add to the available knowledge and investigate clinical implications of OXTR blood concentrations.
Transcription Factor MAFB as a Prognostic Biomarker for the Lung Adenocarcinoma
Authors: Samir Omar , Kobayashi Naohiro , Nishino Teppei , Siyam Mennatullah , Yadav Manoj Kumar , Inoue Yuri , Takahashi Satoru , Hamada Michito
Published: September 2022

MAFB is a basic leucine zipper (bZIP) transcription factor specifically expressed in macrophages. We have previously identified MAFB as a candidate marker for tumor-associated macrophages (TAMs) in human and mouse models. Here, we analyzed single-cell sequencing data of patients with lung adenocarcinoma obtained from the GEO database (GSE131907). Analyzed data showed that general macrophage marker CD68 and macrophage scavenger receptor 1 (CD204) were expressed in TAM and lung tissue macrophage clusters, while transcription factor MAFB was expressed specifically in TAM clusters. Clinical records of 120 patients with lung adenocarcinoma stage I (n = 57), II (n = 21), and III (n = 42) were retrieved from Tsukuba Human Tissue Biobank Center (THB) in the University of Tsukuba Hospital, Japan. Tumor tissues from these patients were extracted and stained with anti-human MAFB antibody, and then MAFB-positive cells relative to the tissue area (MAFB+ cells/tissue area) were morphometrically quantified. Our results indicated that higher numbers of MAFB+ cells significantly correlated to increased local lymph node metastasis (nodal involvement), high recurrence rate, poor pathological stage, increased lymphatic permeation, higher vascular invasion, and pleural infiltration. Moreover, increased amounts of MAFB+ cells were related to poor overall survival and disease-free survival, especially in smokers. These data indicate that MAFB may be a suitable prognostic biomarker for smoker lung cancer patients.
Liquid Biopsy as a Tool for the Diagnosis, Treatment, and Monitoring of Breast Cancer
Authors: Freitas Ana Julia Aguiar de , Causin Rhafaela Lima , Varuzza Muriele Bertagna , Calfa Stéphanie , Hidalgo Filho Cassio Murilo Trovo , Komoto Tatiana Takahasi , Souza Cristiano de Pádua , Marques Márcia Maria Chiquitelli
Published: September 2022

Breast cancer (BC) is a highly heterogeneous disease. The treatment of BC is complicated owing to intratumoral complexity. Tissue biopsy and immunohistochemistry are the current gold standard techniques to guide breast cancer therapy; however, these techniques do not assess tumoral molecular heterogeneity. Personalized medicine aims to overcome these biological and clinical complexities. Advances in techniques and computational analyses have enabled increasingly sensitive, specific, and accurate application of liquid biopsy. Such progress has ushered in a new era in precision medicine, where the objective is personalized treatment of breast cancer, early screening, accurate diagnosis and prognosis, relapse detection, longitudinal monitoring, and drug selection. Liquid biopsy can be defined as the sampling of components of tumor cells that are released from a tumor and/or metastatic deposits into the blood, urine, feces, saliva, and other biological substances. Such components include circulating tumor cells (CTCs), circulating tumor DNA (ctDNA) or circulating tumor RNA (ctRNA), platelets, and exosomes. This review aims to highlight the role of liquid biopsy in breast cancer and precision medicine.
The Landscape of Accessible Chromatin during Yak Adipocyte Differentiation
Authors: Zhang Zhilong , Zhang Yongfeng , Bao Qi , Gu Yarong , Liang Chunnian , Chu Min , Guo Xian , Bao Pengjia , Yan Ping
Published: September 2022

Although significant advancement has been made in the study of adipogenesis, knowledge about how chromatin accessibility regulates yak adipogenesis is lacking. We here described genome-wide dynamic chromatin accessibility in preadipocytes and adipocytes by using the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and thus revealed the unique characteristics of open chromatin during yak adipocyte differentiation. The chromatin accessibility of preadipocytes and adipocytes exhibited a similar genomic distribution, displaying a preferential location within the intergenic region, intron, and promoter. The pathway enrichment analysis identified that genes with differential chromatin accessibility were involved in adipogenic metabolism regulation pathways, such as the peroxisome proliferator activated receptor-γ (PPAR) signaling pathway, wingless-type MMTV integration site (Wnt) signaling pathway, and extracellular matrix-receptor (ECM–receptor) interaction. Integration of ATAC-seq and mRNA-seq revealed that genes with a high expression were associated with high levels of chromatin accessibility, especially within 1 kb upstream and downstream of the transcription start site. In addition, we identified a series of transcription factors (TFs) related to adipogenesis and created the TF regulatory network, providing the possible interactions between TFs during yak adipogenesis. This study is crucial for advancing the understanding of transcriptional regulatory mechanisms of adipogenesis and provides valuable information for understanding the adaptation of plateau species to high-altitude environments by maintaining whole body homeostasis through fat metabolism.
The Role of Tryptophan Metabolites in Neuropsychiatric Disorders
Authors: Davidson Majid , Rashidi Niloufar , Nurgali Kulmira , Apostolopoulos Vasso
Published: September 2022

In recent decades, neuropsychiatric disorders such as major depressive disorder, schizophrenia, bipolar, etc., have become a global health concern, causing various detrimental influences on patients. Tryptophan is an important amino acid that plays an indisputable role in several physiological processes, including neuronal function and immunity. Tryptophan’s metabolism process in the human body occurs using different pathways, including the kynurenine and serotonin pathways. Furthermore, other biologically active components, such as serotonin, melatonin, and niacin, are by-products of Tryptophan pathways. Current evidence suggests that a functional imbalance in the synthesis of Tryptophan metabolites causes the appearance of pathophysiologic mechanisms that leads to various neuropsychiatric diseases. This review summarizes the pharmacological influences of tryptophan and its metabolites on the development of neuropsychiatric disorders. In addition, tryptophan and its metabolites quantification following the neurotransmitters precursor are highlighted. Eventually, the efficiency of various biomarkers such as inflammatory, protein, electrophysiological, genetic, and proteomic biomarkers in the diagnosis/treatment of neuropsychiatric disorders was discussed to understand the biomarker application in the detection/treatment of various diseases.
The Development of Honey Recognition Models Based on the Association between ATR-IR Spectroscopy and Advanced Statistical Tools
Authors: David Maria , Hategan Ariana Raluca , Berghian-Grosan Camelia , Magdas Dana Alina
Published: September 2022

The newly developed prediction models, having the aim to classify Romanian honey samples by associating ATR-FTIR spectral data and the statistical method, PLS-DA, led to reliable differentiations among the samples, in terms of botanical and geographical origin and harvesting year. Based on this approach, 105 out of 109 honey samples were correctly attributed, leading to true positive rates of 95% and 97% accuracy for the harvesting differentiation model. For the botanical origin classification, 83% of the investigated samples were correctly predicted, when four honey varieties were simultaneously discriminated. The geographical assessment was achieved in a percentage of 91% for the Transylvanian samples and 85% of those produced in other regions, with overall accuracy of 88% in the cross-validation procedure. The signals, based on which the best classification models were achieved, allowed the identification of the most significant compounds for each performed discrimination.
Development and Characterization of a Novel Soil Amendment Based on Biomass Fly Ash Encapsulated in Calcium Alginate Microspheres
Authors: Vinceković Marko , Šegota Suzana , Jurić Slaven , Harja Maria , Ondrasek Gabrijel
Published: September 2022

Biomass fly ash (BFA) from a biomass cogeneration plant was encapsulated into calcium alginate microspheres (ALG/Ca) and characterized. An FTIR analysis indicated that BFA loading weakened molecular interactions between ALG/Ca constituents (mainly hydrogen bonding and electrostatic interactions), thus changing the crosslinking density. SEM and AFM analyses revealed a wrinkled and rough surface with elongated and distorted granules. The in vitro release of BFA’s main components (K, Ca, and Mg) was controlled by diffusion through the gel-like matrix, but the kinetics and released amounts differed significantly. The smaller released amounts and slower release rates of Ca and Mg compared to K resulted from the differences in the solubility of their minerals as well as from the interactions of divalent cations with alginate chains. The physicochemical properties of the novel microsphere formulation reveal significant potential for the prolonged delivery of nutrients to crops in a safe manner.
Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis
Authors: Matloch Zdenek , Mraz Milos , Kasperova Barbora Judita , Kratochvilova Helena , Svoboda Petr , Pleyerova Iveta , Reznickova Katerina , Norman Sarah , Hlavacek Daniel , Mahrik Jakub , Ivak Peter , Lacinova Zdenka , Netuka Ivan , Haluzik Martin
Published: September 2022

(1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-α and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.
Transgenic Overexpression of Myocilin Leads to Variable Ocular Anterior Segment and Retinal Alterations Associated with Extracellular Matrix Abnormalities in Adult Zebrafish
Authors: Atienzar-Aroca Raquel , Ferre-Fernández Jesús-José , Tevar Angel , Bonet-Fernández Juan-Manuel , Cabañero María-José , Ruiz-Pastor María-José , Cuenca Nicolás , Aroca-Aguilar José-Daniel , Escribano Julio
Published: September 2022

Myocilin is an enigmatic glaucoma-associated glycoprotein whose biological role remains incompletely understood. To gain novel insight into its normal function, we used transposon-mediated transgenesis to generate the first zebrafish line stably overexpressing myocilin [Tg(actb1:myoc-2A-mCherry)]. qPCR showed an approximately four-fold increased myocilin expression in transgenic zebrafish embryos (144 hpf). Adult (13 months old) transgenic animals displayed variable and age-dependent ocular anterior segment alterations. Almost 60% of two-year-old male, but not female, transgenic zebrafish developed enlarged eyes with severe asymmetrical and variable abnormalities in the anterior segment, characterized by corneal limbus hypertrophy, and thickening of the cornea, iris, annular ligament and lens capsule. The most severe phenotype presented small or absent ocular anterior chamber and pupils, due to iris overgrowth along with dysplastic retinal growth and optic nerve hypertrophy. Immunohistochemistry revealed increased presence of myocilin in most altered ocular tissues of adult transgenic animals, as well as signs of retinal gliosis and expanded ganglion cells and nerve fibers. The preliminary results indicate that these cells contributed to retinal dysplasia. Visual impairment was demonstrated in all old male transgenic zebrafish. Transcriptomic analysis of the abnormal transgenic eyes identified disrupted expression of genes involved in lens, muscular and extracellular matrix activities, among other processes. In summary, the developed transgenic zebrafish provides a new tool to investigate this puzzling protein and provides evidence for the role of zebrafish myocilin in ocular anterior segment and retinal biology, through the influence of extracellular matrix organization and cellular proliferation.
Automatically Generated Visual Profiles of Code Solutions as Feedback for Students
Authors: Swacha Jakub
Published: September 2022

Providing students feedback on their exercise solutions is a crucial element of computer programming education. Such feedback can be generated automatically and can take various forms. This paper introduces and proposes the use of visual profiles of code solutions as a form of automatically generated feedback to programming students. The visual profiles are based on the frequency of code elements belonging to six distinct classes. The core idea is to visually compare a profile of a student-submitted solution code to the range of profiles of accepted solutions (including both reference solutions provided by instructors and solutions submitted by students who successfully passed the same exercise earlier). The advantages of the proposed approach are demonstrated on a number of examples based on real-world data.
Genomic-Phenomic Reciprocal Illumination: Desyopone hereon gen. et sp. nov., an Exceptional Aneuretine-like Fossil Ant from Ethiopian Amber (Hymenoptera: Formicidae: Ponerinae)
Authors: Boudinot Brendon E. , Richter Adrian K. , Hammel Jörg U. , Szwedo Jacek , Bojarski Błażej , Perrichot Vincent
Published: September 2022

Fossils are critical for understanding the evolutionary diversification, turnover, and morphological disparification of extant lineages. While fossils cannot be sequenced, phenome-scale data may be generated using micro-computed tomography (µ-CT), thus revealing hidden structures and internal anatomy, when preserved. Here, we adduce the male caste of a new fossil ant species from Miocene Ethiopian amber that resembles members of the Aneuretinae, matching the operational definition of the subfamily. Through the use of synchrotron radiation for µ-CT, we critically test the aneuretine-identity hypothesis. Our results indicate that the new fossils do not belong to the Aneuretinae, but rather the Ponerini (Ponerinae). Informed by recent phylogenomic studies, we were able to place the fossils close to the extant genus Cryptopone based on logical character analysis, with the two uniquely sharing absence of the subpetiolar process among all ponerine genera. Consequently, we: (1) revise the male-based key to the global ant subfamilies; (2) revise the definitions of Aneuretinae, Ponerinae, Platythyreini, and Ponerini; (3) discuss the evolution of ant mandibles; and (4) describe the fossils as †Desyopone hereon gen. et sp. nov. Our study highlights the value of males for ant systematics and the tremendous potential of phenomic imaging technologies for the study of ant evolution.
Ultrastructure Characteristics and Sexual Dimorphism of Antennal Sensilla in Tirathaba rufivena (Lepidoptera: Pyralidae)
Authors: Guo Jixing , Du Zimeng , Cui Guangchao , Wang Zheng , Wang Junfang , Zhou Xiang
Published: September 2022

Tirathaba rufivena Walker, a major insect pest of Areca catechu L., has severely threatened areca nut cultivation in Hainan, China. To improve our understanding of the communication mechanism in host plant seeking and mate-finding for T. rufivena, we described and further characterized the external morphology and internal sensilla structures using scanning electron microscopy and transmission electron microscopy in this study. The antennal morphology was similar between males and females, and there was no significant difference in length between the two sexes. In total, nine sensilla types were identified: sensilla trichodea (Str), sensilla chaetica (Sch), sensilla basiconica (Sba), sensilla auricillica (Sau), sensilla coeloconica (Sco), sensilla styloconica (Sst), Böhm sensilla (Bs), uniporous peg sensilla (Ups) and sensilla squamiformia (Ssq). Sexual dimorphism mainly occurs in variation in the length of Sba, Sch, Sco1 and Bs, and the abundance of Sba, Sau1 and Sau2. The Sba had larger size and numbers on female antennae than that on males, suggesting that these sensilla might have important roles in locating host plants. Both Sau1 and Sau2 were significantly more abundant in females and were probably associated with the detection of mates and host plant for oviposition. These data were important for ongoing studies on host plant seeking and mate-finding behavior in T. rufivena and provided a theoretical foundation to further studies of semiochemical control for this pest.