bluuebunny/crossref_metadata_2025 huggingface dataset:
IMPLIKASI PENANGANAN MASALAH COMFORT WOMEN TERHADAP HUBUNGAN JEPANG DAN KOREA SELATAN PADA TAHUN 2015-2019 [THE IMPACT OF RESOLVING THE COMFORT WOMEN ISSUE TO JAPAN AND SOUTH KOREA RELATIONS DURING 2015 - 2019]
Authors: Gracellia Jennifer
Published: June 2020

The issue of comfort women that has occured since 1932 continues to impact bilateral relations between Japan dan South Korea. Various efforts have been made by two countries to deal with this issue, one of which is the agreement in 2015 that stating the comfort women issue has been completed and this agreement cannot be canceled. Instead of solving the problem, this agreement marked as the beginning of a worsening relation between the two countries. Poor relations led to several implications which then became a new problem to Japan and South Korea relations. This research finds that the comfort women issue has given three implications for the relations between Japan and South Korea. First is the Japan-South Korea Trade War in 2019, second is the withdrawal of South Korea from General Security of Military Information Agreement (GSOMIA) and the last is the boycott of Japanese products conducted by South Korean society. The unresolved issue has affected the economy, national security and the social life of the people of both countries.

BAHASA INDONESIA ABSTRAK: Permasalahan comfort women yang terjadi sejak tahun 1932 terus memberikan implikasi yang kuat kepada hubungan bilateral antara Jepang dan Korea Selatan. Berbagai usaha telah dilakukan oleh kedua negara untuk menangani permasalahan ini, dimana salah satunya adalah perjanjian pada tahun 2015 yang menyatakan permasalahan comfort women telah selesai dan perjanjian ini tidak dapat dibatalkan. Bukannya menyelesaikan masalah, perjanjian ini menjadi awal dari hubungan kedua negara yang semakin memburuk. Hubungan yang buruk kemudian menimbulkan beberapa implikasi yang menjadi masalah baru di dalam hubungan Jepang dengan Korea Selatan. Berdasarkan hasil penelitian yang telah dilakukan, ditemukan bahwa permasalahan comfort women memberikan tiga implikasi kepada hubungan Jepang dan Korea Selatan yaitu Perang Dagang Jepang-Korea Selatan 2019, penarikan Korea Selatan dari General Security of Military Information Agreement (GSOMIA) dan pemboikotan produk Jepang yang dilakukan oleh masyarakat Korea Selatan. Permasalahan comfort women yang tidak kunjung terselesaikan telah mempengaruhi perekonomian, keamanan nasional, hingga kehidupan sosial masyarakat kedua negara.
Bermain bunga kamboja sambil berhitung pada anak usia 5-6 tahun
Authors: Abdurrahman Abdurrahman , Suarti Ni Ketut Alit , Gunawan I Made
Published: June 2020

[Bahasa]: Pelaksanaan pengabdian bagi Masyarakat ini bertujuan untuk memberikan pemahaman kepada orang tua tentang manfaat bunga kamboja dapat menjadikan anak cerdasa dalam berhitung di lingkungan masyarakat, Kota Mataram NTB. Diharpkan melalui kegiatan ini, dapat meningkatkan pemahaman tentang pengetahuan media ajar serta perkembangan anak hususnya pada kecerdasan berhitung (logic matematik) yang diterapkan sejak dini. Pelaksanaan pengabdian  ini direncanakan akan berjalan dengan sasaran utama adalah masyarakat/orang tua, anak didik sekolah PAUD di lingkuangan masyarakt. Metode pelaksanaan kegiatan ini melalui sosialisai, persiapan kegiatan, penyuluhan, pelatihan serta pendampingan. Sedangkan prosedur kegiatan yakni perencanaan (planning), Pelaksanaan (actuating) yang meliputi (tahap I, II, dan III) dan pengawasan (controlling), serta diadakan evalusi dan keberlanjutannya dengan menggunakan instrumen yang sudah disiapkan. Hasil dari kegiatan ini adalah pemahaman orang tua tentang media ajar serta perkembangan berhitung anak.

Kata Kunci: pelatihan, media ajar dengan tanah liat, kemampuan berhitung anak
Magnetic Attachment Retained Complete Overdenture as Treatment for Maintaning Alveolar Ridge Height – A case report
Authors: Angdrijono Anita , Herdijantini Nike , Eka Hanoem
Published: June 2020

Background:  Magnetic attachment can be used as  a retentive devices in overdenture technique in prostheses. The magnets are attached with remaining root structure transfer the occlusal load to the bone through the periodontal ligament of retained roots and then prevent resorption of the  alveolar ridge. Alvelar ridge plays an important role for the retention and stability of the denture as they will support the denture base. Magnetic assembly consist of magnet and coping with a keeper on the remaining tooth structure  since magnetic attachment can provide support, stability and retention. Purpose: The purpose of this case report was to report magnetic attachment retained complete overdenture design and fabrication as a preserve to alveolar bone height to achieve retention and stability of the denture.  Case: A 69 years old female patient, a baker and private English teacher with partial edentulous ridge came to Dental Hospital of Airlangga University asked for dentures. Clinical examination shown a complete edentulous in the upper jaw and partial edentulous in the lower jaw. The remaining teeth were 33, 34, 35, 43 and 44. 33 and 35 are chronic gangrene radix, 35 shown a slightly mobility. 34 non vital, 44 and 45 are vital with moderate resorption. Case management : All the left teeth in the lower jaw were consulted for endodontic treatment. Magnetic attachment retained complete overdenture was choosen for the lower jaw and complete denture for the upper jaw. The remaining teeth in the lower jaw kept the alveolar ridge from resorption and the magnet provided extra retention for the complete overdenture. Discussion: Overdentures supported by magnet attachment achieved greater satisfaction, gives better retention  and stability for the denture. On the other side, the natural abutment teeth in overdentures  preserved  better proprioception and psychologically beneficial as the patient had not undergone extraction. As a conclusion, overdenture supported by magnetic attachment can be used to increase support, retention and stability especially in the lower jaw and also  prevent further alveolar ridge resorption. Conclusion: Overdenture supported by magnetic attachment can be used to increase retention and the residual root also prevent further alveolar ridge resorption.
Analysis of Soft Tissue Cephalometry in Skeletal Class I with Post Operation Unilateral and Bilateral CLP
Authors: Fitrianto Ardan , Hamid Thalca , Narmada Ida Bagus
Published: June 2020

Background: Facial appearance is an important diagnostic criterion that must be considered in orthodontics treatment plan. Orthodontics treatment is one of the dental treatments to prevent or correct tooth position abnormalities so that optimal function can be achieved including occlusion, proportional arrangement of the teeth and facial profile, as well as the harmony of facial profiles. Common facial abnormality cases include cleft lip and palate. Cleft lip and palate are caused by congenital defects and environmental factors. Purpose: The study was aimed to determine post-operative soft tissue cephalometric analysis of skeletal class I with post-operative of unilateral and bilateral CLP. Methods: This was a descriptive observational study. The subjects were secondary data from radiographic cephalometry obtained from the CLP Center Premier Hospital Surabaya and Universitas Airlangga Dental Hospital. Result: There was a significant difference in line angle parameters in both groups with a significant value of 0.002 (p <0.05). There were also significant differences in the Li-H line parameters in both groups with a significant value of 0.000 (p <0.05). There were H line angle and Li-H line differences in soft tissue cephalometric analysis between skeletal class I group with post-operative unilateral and bilateral CLP group. Conclusion: There was no difference in soft tissue cephalometric analysis between the post-operative of unilateral CLP and bilateral CLP on all parameters.
The Effect of Combination of Soy Milk and Calcium in Orthodontic Tooth Movements on Female Rats With Estrogen Deficiency
Authors: Ramadhani Vanda , Narmada Ida Bagus , Hamid Thalca
Published: June 2020

Background: Orthodontic treatments in elderly patients are influenced by hormonal changes and their effects on the process and prognosis of the treatment. Estrogen deficiency in the post-menopausal period results in bone loss. The process of bone remodeling during Orthodontic treatment for elderly patients is also influenced by the balance of resorption and bone formation processes. Purpose: This study aimed to determine the effect of soymilk combination calcium on orthodontic tooth movement in female rats with estrogen deficiency. Methods: Twenty rats were divided into 5 groups with 4 samples for each group. All samples were performed bilateral ovariectomy. The control group (K) was observed on day 0, P1 group was observed on day 7, P2 group was observed on day 14, P3 group was observed on day 7, and P4 group was observed on day 14. NiTi closed coil spring was placed between the incisor and maxillary molars to move the molars toward the mesial. Osterix and ALP expression were analyzed using SPSS program. Results: The results showed that the highest number of osteoblasts, Osterix and ALP expression was found from the group soy milk and calcium intake in 14 days observation (P4) with a mean ± SD Osterix: 15.75 ± 0.2600, and ALP with a value of 12.60 ± 1,220. Conclusion: Soy milk combination calcium has a very good effect to balance the hormonal condition and bone remodelling for elderly patient or menopause, maintaining bone stability and density during orthodontic treatment.
The Effect of Addition of Bambusa Arundinacea (Retz.) Willd. Fibers to Heat-Cured Acrylic Resin on Impact Strength
Authors: Elonianty Chanita , Agustantina Titien Hary , Nirwana Intan
Published: June 2020

Background: The prevalence of acrylic resin dentures fracture due to accidental drop is relatively high especially when it is being cleaned by patient. The effort to strengthen acrylic resin denture was done by adding Bambusa arundinacea (Retz.) Willd fibers, which was based on previous research. It has been proven that the addition of bamboo fiber to self-cured acrylic resin can increase the tensile strength. It has acceptable tensile properties, relatively low cost, high toughness, and good thermal properties. Purpose: To determine the impact strength of heat-cured acrylic resin after the addition of Bambusa arundinacea (Retz.) Willd. fibers at various lengths and weights. Methods: Bambusa arundinacea (Retz.) Willd. fibers with lengths of 2 mm, 6 mm, 12 mm and weights of 3wt%, 6wt%, 9wt% were added to heat-cured acrylic resin. The impact strength of heat-cured acrylic resin was tested using the Charpy method with mini impact tester KRY type. The test results were calculated using the impact strength formula. The research data were analyzed using one-way anova test and Tukey HSD test. Results: The data analysis showed that there was a significant difference in impact strength of heat-cured acrylic resin after the addition of Bambusa arundinacea (Retz.) Willd fibers. Conclusion: Addition of Bambusa arudinacea (Retz.) Willd. fibers increased the impact strength of heat-cured acrylic resin respective to the increment of length and weight of Bambusa arudinacea (Retz.) Willd. fibers.
Multifonctionnalité des prairies : comment articuler marché et politiques publiques ?
Authors: LE GOFFE P.
Published: June 2020

Du point de vue de la société et de l’efficacité économique, les agriculteurs n’utilisent pas assez les surfaces en prairies en raison d’incitations inexistantes ou contradictoires. D’une part, les aides directes de la PAC les incitent à favoriser les surfaces en cultures au détriment des prairies. D’autre part, l’agriculteur, qui cherche à maximiser son profit, ne prend pas en compte les externalités des prairies (positives) et des cultures (négatives), en raison de l’absence d’incitations marchandes. Le marché pourrait pourtant contribuer à l’utilisation des surfaces en prairies que la société attend, car le lait et la viande produits à l’herbe ont des caractéristiques particulières qui peuvent être valorisées auprès des consommateurs. Par ailleurs, les prairies rendent de nombreux services collectifs à la société : lutte contre l’érosion, amélioration du régime et de la qualité des eaux, biodiversité… Ces caractéristiques de bien public des prairies font que des défaillances du marché demeurent et que des politiques publiques sont nécessaires. La conception de politiques de la multifonctionnalité des prairies nécessite l’étude des mécanismes de la production conjointe de produits animaux et de biens publics associés aux prairies, et celle des coûts de transaction. Ces coûts, qui sont relatifs aux transactions marchandes mais aussi à la mise en œuvre des politiques publiques, conditionnent largement l’efficacité du marché et des politiques. Les différentes formes de prime à l’herbe permettent probablement de générer des bénéfices environnementaux sans augmenter exagérément les coûts de transaction par rapport à des politiques de soutien des prix.
Efficacité des traitements de synchronisation des chaleurs chez les bovins
Authors: GRIMARD B. , HUMBLOT P. , PONTER A.A. , CHASTANT S. , CONSTANT F. , MIALOT J.P.
Published: June 2020

En France, trois types de traitements hormonaux permettent de synchroniser les chaleurs chez les bovins. Les traitements à base de prostaglandine F2α ou de ses analogues (2 injections à 11-14 jours d’intervalle), les traitements associant GnRH et PGF2α (Ovsynch : GnRH à J0, PGF2α à J7, GnRH à J9), les traitements à base de progestagènes (dispositif libérant de la progestérone ou du norgestomet associé à un œstrogène et/ou à des PGF2α et de l’eCG). La prostaglandine F2α et ses analogues provoquent la lutéolyse et les chaleurs qui suivent la deuxième injection s’étalent sur plusieurs jours l’insémination sur chaleurs observées après ce traitement améliore les résultats de fertilité. Les associations GnRH et prostaglandine et progestagène/œstrogène/eCG agissent à la fois sur la croissance folliculaire et sur la lutéolyse ; les chaleurs qui apparaissent après traitement sont bien groupées et il est possible d’inséminer en aveugle. Compte tenu de leur mécanisme d’action, le premier traitement est réservé aux animaux cyclés alors que les deux traitements GnRH + prostaglandine (Ovsynch) et progestagènes peuvent être utilisés sur des animaux cyclés ou non (anoestrus). La fertilité à l’oestrus induit est variable, de 20 à 70 % sur de grands lots d’animaux. Certains facteurs de variation sont connus mais non maîtrisables dans la pratique au moment de la mise à la reproduction (jour du cycle au moment du traitement, cyclicité avant traitement, rang de vêlage, conditions de vêlage, note d’état corporel…). D’autres peuvent être utilisés pour améliorer la fertilité des animaux à risque (allonger l’intervalle vêlage-traitement, pratiquer un flushing, séparer temporairement le veau). Ainsi, une bonne connaissance des mécanismes physiologiques expliquant l’effet de chaque traitement, une évaluation des facteurs de risque présentés par les animaux à synchroniser et l’application de mesures correctrices devraient permettre d’adapter les traitements aux situations rencontrées (cyclées, anoestrus, suboestrus, troupeau laitier ou allaitant …), afin d’améliorer la fertilité et de réduire la variabilité de la réponse au traitement.
L’élevage bovin et l’environnement en France : le diagnostic justifie-t-il des alternatives techniques ?
Authors: CHATELLIER V. , VERITE R.
Published: June 2020

Les relations entre l’élevage bovin et l’environnement sont complexes avec des implications positives (occupation du territoire, forte autonomie du système alimentaire, maintien de la biodiversité) et d’autres négatives (augmentation des teneurs en nitrates et en phosphore des eaux, émission de gaz à effet de serre). Ces relations sont historiquement et localement étroitement dépendantes de facteurs techniques (niveau d’intensification des superficies fourragères, productivité des facteurs de production, plans de fertilisation, conditions de stockage des effluents d’élevage…). Elles sont également fortement influencées par certaines considérations économiques (évolution de la consommation, prise en compte dans le prix des produits du respect d’engagements environnementaux…) et politiques (fixation de normes environnementales, mode d’intervention des pouvoirs publics dans la gestion collective de l’offre et dans la répartition territoriale des productions, mécanisme d’attribution des droits à primes…). L’analyse de ces relations est rendue délicate par la diversité des systèmes de production et l’imbrication des problèmes de gestion du végétal et de l’animal. Outre les incitations incombant aux politiques agricoles, plusieurs évolutions techniques pourraient interagir dans le sens d’une amélioration de la relation à l’environnement. Ces évolutions techniques, ponctuelles (suppression des gaspillages et ajustement plus précis des intrants, gestion des déjections…) ou plus radicales (modification des systèmes des production), seront possibles si elles s’accompagnent d’une amélioration sociale et/ou économique à l’échelle de l’exploitation.
La conduite de la reproduction du troupeau de vaches allaitantes : modélisation des prises de décision
Authors: INGRAND S. , COURNUT S. , DEDIEU B. , ANTHEAUME F.
Published: June 2020

Ce travail, de type méthodologique, a comme objectif d’analyser les prises de décision pour la conduite du troupeau de vaches dans les élevages bovins allaitants : règles, occurrences, inter-relations, liens avec le projet de production. La conduite du troupeau a été décrite et formalisée à l’issue d’enquêtes (2 passages) réalisées dans 7 élevages de type naisseur et 5 élevages de type naisseur/engraisseur en zone Charolaise (Saône-et-Loire, Allier). Trois catégories d’indicateurs mobilisés par les éleveurs ont été répertoriées : indicateurs de l’état instantané des animaux, indicateurs différés portant sur la carrière antérieure de l’animal et indicateurs exogènes, indépendants des animaux (état de la ressource alimentaire, contexte économique). Selon le projet de production, ces indicateurs sont mobilisés différemment par les éleveurs et 4 prototypes de conduite du troupeau ont été définis. Les informations ainsi répertoriées doivent être utilisées dans le cadre de la construction d’un simulateur informatique du fonctionnement des troupeaux dans ces élevages.
(only in French) Caractéristiques nutritionnelles des produits laitiers et variations selon leur origine
Authors: COULON J.B. , ROCK E. , NOËL Y.
Published: June 2020

Au cours des dernières années de nombreux travaux ont été réalisés sur les caractéristiques sensorielles des fromages d’AOC, dans le cadre général de l’objectivation de leur liaison à leur terroir (voir Martin et al 2003, dans ce même dossier). De plus en plus, et au-delà des produits AOC, les consommateurs sont à la recherche d’information sur les caractéristiques nutritionnelles des aliments qu’ils consomment, et des produits laitiers en particulier. Les fromages d’AOC sont particulièrement concernés par cette question, dans la mesure où les conditions de leur production peuvent conduire à des caractéristiques nutritionnelles spécifiques. Celles-ci relèvent schématiquement de deux domaines distincts, d’une part les caractéristiques liées à la microbiologie des laits et des fromages et d’autre part celles liées à leur teneur en macro ou micro-constituants d’intérêt nutritionnel (protéines, lipides, vitamines, minéraux…).L’objectif de ce texte est de fournir quelques points de repères sur le rôle des micronutriments et de la microflore sur la santé, sur les liens entre la microflore digestive de l’Homme et son système immunitaire, et sur les actions déjà engagées ou en projet pour identifier et comprendre le rôle de la consommation de fromages au lait cru sur la santé humaine. Voir la suite de l'article à l'adresse :https://www6.inrae.fr/productions-animales_eng/content/download/3821/39526/version/1/file/Prod_Anim_2003_16_4_05.pdf
CRISPR-Based Assays for Rapid Detection of SARS-CoV-2
Authors: Javalkote Vivek S. , Kancharla Nagesh , Bhadra Bhaskar , Shukla Manish , Soni Badrish , Sapre Ajit , Goodin Michael , Bandyopadhyay Anindya , Dasgupta Santanu
Published: June 2020

COVID-19 represents an unprecedented threat to global public health and economies. Assays are urgently needed for rapid detection and diagnosis of SARS-CoV-2-infected patients in order to inform treatment and quarantine strategies. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid-based tests are considered to be more sensitive with respect to serological tests, but the present gold standard RT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. Novel CRISPR- and other nuclease-based techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here we discuss and present compilation of state-of-the-art CRISPR based detection techniques for use in COVID diagnosis and epidemiology.
Avaliação de diferentes fontes de carboidratos para o sistema de bioflocos e crescimento do camarão branco
Authors: GANDINI Fernanda Alves , NASCIMENTO júnior José Ricardo de Oliveira , MEDEIROS Cataline Soares , OSHIRO Lidia Miyako Yoshii , SANT'ANA Nivaldo De Faria
Published: June 2020

O trabalho teve como objetivo avaliar o resíduo de cervejaria e outras diferentes fontes de carboidratos para o sistema de bioflocos e  diferentes densidades para o crescimento do camarão branco, Litopenaeus vannamei (peso inicial de 0,35 ± 0,05g). O experimento foi realizado durante o período de 55 dias, utilizando 6 tratamentos com 3 repetições cada, com duas densidades de estocagem (300 e 500 camarões/m²) e três substratos (resíduo de cervejaria, melaço de cana e farinha de mandioca). Os parâmetros abióticos foram monitorados diariamente e as biometrias ocorreram semanalmente, para a avaliação do desempenho. Os parâmetros físico-químicos da água encontraram-se adequados para a espécie, entretanto os compostos nitrogenados alcançaram níveis pouco elevados, porém os índices zootécnicos foram satisfatórios, apresentando médias de sobrevivência, ganho de peso e peso final entre 74-82%; 3,5-5,0g e 4,2-5,7g, respectivamente. Verificou-se que tratamentos com o resíduo de cervejaria apresentaram parâmetros zootécnicos superiores e também, maior formação de flocos microbianos. Os tratamentos com menores densidades apresentaram melhores índices zootécnicos. Os resultados demonstraram que a produtividade dos flocos nos diferentes tratamentos é eficiente como complemento alimentar para o crescimento de L. vannamei e para a qualidade da água, e que entre os substratos, o resíduo de cervejaria revela-se como excelente composto orgânico para a formação do bioflocos.
Quem emergirá? o País ou os contaminantes: um estudo sobre a presença de parabenos em ambientes lacustres costeiros.
Authors: Vedana Jaísa , Alves Thiago , Beaumord Antônio
Published: June 2020

Os parabenos são conservantes químicos comumente encontrados em produtos de higiene pessoal, medicamentos e alimentos. Eles são introduzidos no meio ambiente através de atividades antropogênicas. Esse contaminante afeta negativamente o meio ambiente e todos os organismos (incluindo o humano). As incertezas sobre quantidades, usos e descarte de contaminantes são assuntos de grandes preocupações. Portanto, esta pesquisa tem como objetivo avaliar a ocorrência de parabenos e sua possível relação com a urbanização e o clima em ambientes aquáticos. Foram investigadas duas massas de água doce, Lagoa Pequena e Lagoa do Peri, seus canais de transbordamento e pontos marinhos próximos aos canais, em 14 pontos de amostragem. A análise da quantificação de parabenos foi realizada utilizando cromatografia líquida de alta eficiência (HPLC-DAD). Os resultados indicaram que em todos os pontos de amostragem, nos dois ambientes, as concentrações de parabenos foram associadas à intensidade da ocupação antropogênica. Nos pontos de amostragem altamente perturbados em Lagoa Pequena e Lagoa do Peri, as concentrações de parabenos variam de 5,5 a 6,9 ɳg.L-1, respectivamente. Devido à ausência de limites desses poluentes na legislação ambiental brasileira, não é possível estabelecer uma referência sobre os valores observados nesses ambientes e, portanto, a extensão de seus efeitos nos organismos em curto e longo prazo.
Gelo Marinho Antártico e a Trajetória de Ciclones sobre o Oceano Atlântico do Sul
Authors: Gandra Mariana , Quadro Mário Francisco Leal de
Published: June 2020

Os ciclones desempenham um papel importante na circulação geral da atmosfera, possibilitando os transportes meridionais de calor, umidade e momento excedentes das baixas latitudes para altas latitudes. No Atlântico Sul as áreas mais favoráveis para a formação de ciclones são próximo à costa leste da América do Sul (30°S a 45°S) e Península Antártica. Um dos fatores que favorecem a formação da ciclogênese na costa leste da América do Sul é o contraste de temperatura entre o oceano Atlântico Sul com a camada de ar superficial. Enquanto que a ciclogênese próxima à Península Antártica pode estar associada a instabilidades baroclínicas pré existentes em níveis mais altos da atmosfera. O objetivo deste trabalho é investigar qual o papel da Península Antártica no comportamento dos ciclones extratropicais que atuam sobre a América do sul e oceanos adjacentes. Para tal, foi aplicado o algoritmo TRACK para rastreamento dos ciclones. A interação oceano-atmosfera será investigada por meio do modo oceano-atmosfera do modelo acoplado COAWST. A execução do TRACK se mostrou satisfatória, uma vez que os resultados encontrados corroboram com a literatura da região.
Association of complete uncinate process removal on 2-year assessment of radiologic outcomes: subsidence and sagittal balance in patients receiving one-level anterior cervical discectomy and fusion
Authors: Noh Sung Hyun , Park Jeong Yoon , Kuh Sung Uk , Chin Dong Kyu , Kim Keun Su , Cho Yong Eun , Kim Kyung Hyun
Published: June 2020

Background: Many patients with cervical radiculopathy experience stenosis of the neural foramens due to cumulative osteophyte or uncovertebral joint hypertrophy. For cervical foraminal stenosis, complete uncinate process resection (UPR) is often conducted concurrently with anterior discectomy and fusion (ACDF). The aim of this study was to assess the clinical and radiological outcomes of ACDF with complete UPR versus ACDF without UPR. Methods: In total, 105 patients who performed one-level ACDF with a cage-and-plate construct between 2011 and 2015 were retrospectively reviewed. Among them, 37 patients had ACDF with complete UPR, and 68 patients had ACDF without UPR. Radiologic outcomes of disc height, C2–C7 lordosis, T1 slope, C2–C7 sagittal vertical axis (SVA), center of the sella turcica–C7 SVA (St-SVA), spino-cranial angle (SCA), and fusion rate were evaluated on plain X-ray at pre-operation, immediately post-operation, and at 2-year follow-up. For statistically matched pairs analysis, ACDF with UPR group (24 patients) and ACDF without UPR (24 patients) were compared. Results: All of the clinical parameters improved at the 2-year follow up (P<0.0001). Improvement in visual analogue scale (VAS) scores for arm pain was significantly improved in the ACDF with complete UPR group immediately post-operation. All cervical sagittal parameters, including cervical lordosis, segmental angle, disc height, C2-C7 SVA, St-SVA, T1 slope, and SCA, except for preoperative St-SVA, SCA, and disc height of 2 years follow-up, were similar between the ACDF with complete UPR and ACDF without UPR groups. Differences in disc height, C2-C7 SVA, and SCA at 2-year follow up after preoperative examination, however, were statistically significant (p<0.05). Subsidence occurred in 9 patients (ACDF with complete UPR: 8 cases [33%] versus ACDF without UPR: 1 cases [4%]; p < 0.05). Conclusions: Cervical sagittal alignment after ACDF with complete UPR is not significantly different from that achieved with ACDF without UPR. However, subsidence appears to occur more often after ACDF with complete UPR than after ACDF without UPR, although with little to no clinical impact. More precise and careful selection of patients is needed when deciding on additional complete UPR.
Interference of functional dual-tasks on gait in untrained people with Parkinson’s disease and healthy controls: cross-sectional study
Authors: Valenzuela Constanza San Martín , Dueñas-Moscardó Lirios , López-Pascual Juan , Serra-Añó Pilar , Miguel José Manuel Tomás
Published: June 2020

Background: In Parkinson’s disease (PD) population, performing secondary tasks while walking further deteriorates gait and restrict mobility in functional contexts of daily life. This study (1) analyzed the interference of functional cognitive and motor secondary task on untrained people with PD and (2) compared their walking with healthy subjects. Methods: Forty people with PD (aged 66.72 [7.5] years, Hoehn and Yahr stage I-II-III, on-medication) composed the PD group (PDG) and 43 participants (aged 66.60 [8.75] years) formed the group of healthy counterparts (HG). Gait was evaluated through spatiotemporal, kinematic and kinetic outcomes in five conditions: single task (ST) and visual, verbal, auditory and motor dual-task (DT).Results: The velocity, stride length, and braking force performance of both groups was statistically higher in the ST condition than in verbal, auditory and motor DT (p < .05), and inferior in double support time and midstance force (p < .05). The same pattern was observed when compared the ST and visual DT condition, where participants showed a significantly higher stride length, double support time and braking force in the ST (p < .05). In addition, the PDG exhibited a significant shorter double support time and midstance force, and showed a higher braking force in the visual DT than in the verbal DT (p < .05). Similarly, the PDG showed a wider stride in the visual DT than in the motor DT condition (p < .05). PDG participants had a significantly lower performance than the HG in all the variables analyzed except for the maximum hip extension in the stance phase (p > .05). Conclusions: In untrained participants with PD, verbal and motor secondary tasks affect gait significantly, while auditory and visual tasks interfere to a lesser extent. Untrained people with PD have a poorer gait performance than their healthy counterparts, but in different grades according to the analyzed variables. Trial registration: The data in this paper are part of a single-blind, randomized, controlled trial and correspond to the evaluations performed before a physical rehabilitation program, retrospectively registered with the number at clinicaltrial.gov NCT04038866.
Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica
Authors: You Wuxin , Wei Li , Gong Yanhai , Hajjami Mohamed El , Xu Jian , Poetsch Ansgar
Published: June 2020

Background: Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data. Results: Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h. Conclusions: This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.
Neuroprotective Effects of Magnesium L-threonate in a Hypoxic Zebrafish Model
Authors: Kim Young Sung , Won Young Ju , Lim Byung Gun , Min Too Jae , Kim Yeon-Hwa , Lee Il-Ok
Published: June 2020

Background: Hypoxia inhibits the uptake of glutamate (a major neurotransmitter in the brain closely related to cognitive function) into brain cells, and the initial response of cells to cortical hypoxia depends on glutamate. Previous studies have suggested that magnesium may have protective effects against hypoxic injuries. In particular, magnesium L-threonate (MgT) may increase magnesium ion concentrations in the brain better than MgSO4 and improve cognitive function. Methods: We evaluated cell viability under hypoxic conditions in the MgT- and MgSO4-treated human SH-SY5Y neurons, in vivo behavior using the T-maze test following hypoxia in MgT-treated zebrafish, activity of brain mitochondrial dehydrogenase by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and protein expression of the excitatory amino acid transporter (EAAT) 4 glutamate transporter by western blotting.Results: Among the groups treated with hypoxia, cell viability significantly increased when pre-treated with 1 or 10 mM MgT (p=0.009 and 0.026, respectively). Despite hypoxic insult, MgT-treated zebrafish showed preferences for the red compartment (p=0.025 for distance and p=0.007 for frequency of entries), suggesting memory preservation. TTC staining showed reduced cerebral infarction and preserved absorbance in the MgT-treated zebrafish brain after hypoxia (p=0.010 compared to the hypoxia group). In addition, western blot showed upregulation of EAAT4 protein in the MgT treated group.Conclusions: Pre-treatment with MgT attenuated cell death and cerebral infarction due to hypoxia and protected cognitive function in zebrafish. In addition, MgT appeared to modulate expression of the glutamate transporter, EAAT4.
Mortality at the Pediatric Emergency Unit of the Mohammed VI Teaching Hospital of Marrakech
Authors: Lahmini Widad , bourrous mounir
Published: June 2020

Background: The death of a child at the emergency ward is one of the most difficult problems that the clinicians of these wards have to deal with. In our country the published data concerning the causes and the factors related to pediatric mortality especially in the pediatric emergency wards is very rare. This study aimed to study the epidemiology of the pediatric mortality in the pediatric emergency department (PED), to determine its rate and identify its most frequent causes. Methods: It is a retrospective and descriptive study, over five years (1st january 2012 and 31st december 2016) including all children aged from 0 to 15 years old who died at the PED in the Mohamed VI Hospital in Marrakech. Results: During the period of the study a total of 172.691 patients presented to the PED, among which 628 died (pediatric mortality rate : 3.63%). The masculine gender was predominant (n=383) with a gender ratio of 1.59. Two-thirds of the patients died in the first 24 hours (n=421). The median of time from admission to death was around 12 hours. Majority of the deceased children (n=471, 75%) were from a low socioeconomic status. The most frequent cause of admissions for deceased patients in the PED was respiratory distress (n=296, 47%) followed by neurological disorders (n=70, 11%). Neonatal mortality (≤ 1 month of age) was predominant (n=472, 75.1%), followed by postnatal mortality (1 month to 1 year old) (n=73, 11.6%). The most frequent causes of pediatric mortality, whatever the age range, were dominated by neonatal pathologies (n=391, 62.3%), followed by infecious causes bronchopulmonary infections included (n=49, 7.7%), birth deformities (n=46, 7.3%) while traumas were merely at 0.9% (n=6). The most frequent causes of neonatal mortality were neonatal infections (n=152, 32.2%), prematurity (n=115, 24.4%), perinatal asphyxia (n=71,15%) and neonatal respiratory distress (n=57, 12.1%). Conclusion: Our data once again underline the crucial importance of prevention. This requires correct follow-up of the pregnancies, an adequate assistance of births, and perfecting healthcare provision to newborns in order to attain proper assistance.
Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis
Authors: Rodríguez-Alarcón Carlos Arturo , Beristain-Ruiz Diana M. , Olivares-Muñoz Angélica , Quezada-Casasola Andrés , Pérez-Casio Federico , Álvarez-Martínez Jesús A. , Tapia-Alanís Jane , Lira-Amaya José J. , Rivera-Barreno Ramón , Cera-Hurtado Orlando S. , Ibancovichi-Camarillo José A. , Soon-Gómez Luis , Adame-Gallegos Jaime Raul , Figueroa-Millán Julio V.
Published: June 2020

Background: Nowadays, Ehrlichia canis receives more attention because of its great morbidity and mortality in animals. Dogs in the subclinical and chronic phases can be asymptomatic, and serologic tests show cross-reactivity and fail to differentiate between current and past infections. Moreover, there could be low parasitaemia, and E. canis might be found only in target organs, hence negative by PCR in blood. Methods: We evaluated by PCR the prevalence of E. canis in blood, liver, spleen, lymphatic nodules, and bone marrow in 59 recently euthanized dogs that had ticks but were clinically healthy. Results: In total, 52.55% of the blood PCRs for E. canis were negative, yet 61.30% yield positive results in tissue biopsies as follows: 63.15% from bone marrow, 52.63% from liver, 47.36% from spleen and 15.78% from lymphatic nodules. In addition, 33% had infection in three tissues (spleen, liver and bone marrow). Conclusions: Our results show prevalence of E. canis in tissue from dogs that were negative by PCR in blood. E. canis DNA in tissue was 30% lower in dogs that tested negative in blood samples by PCR, compared to those that were positive. However, it must be taken into account that some dogs with negative results were positive for E. canis in others tissues.
Evaluation of the association between presenteeism and perceived availability of social support among hospital doctors in Zhejiang, China
Authors: Xi Xiaoyu , Lu Qianni , Lu Mengqing , Xu Ailin , Hu Hao , Ung Carolina Oi Lam
Published: June 2020

Background: This study investigated the association between presenteeism and the perceived availability of social support among hospital doctors in China. Methods: A questionnaire was administered by doctors randomly selected from 13 hospital in Hangzhou China using stratified sampling. Logit model was used for data analysis. Results: The overall response rate was 88.16%. Among hospital doctors, for each unit increase of the perceived availability of social support, the prevalence of presenteeism was decreased by 8.3% (OR=0.91, P=0.000). In particular, if the doctors perceived availability of appraisal support, belonging support and tangible support as sufficient, the act of presenteeism was reduced by 20.2% (OR=0.806, P=0.000) 20.4% (OR=0.803, P=0.000) and 21.0% (OR=0.799, P=0.000) respectively with statistical differences. Conclusion: In China, appraisal support, belonging support and tangible support, compared to other social support, had a stronger negative correlation with presenteeism among hospital doctors. The benefits of social support in alleviating doctors’ presenteeism warrant further investigation.
Effect of Physical Exercise in Bariatric Surgery Patients: Protocol of a Randomized Controlled Clinical Trial
Authors: Herrera Andrea , Tabach Andrea , Andaur Karen , Zamunér Antonio
Published: June 2020

Background: Bariatric surgery is an effective approach to weight loss and long-term comorbidity resolution. Although recommended in several guidelines, supervised exercise has not been systematically prescribed after bariatric surgery. The aim of this study is to determine the effects of two types of exercise, moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT), on body composition, cardiopulmonary function and perceived quality of life in bariatric surgery patients.Methods: This randomized controlled clinical trial will include 75 adults of both sexes scheduled for bariatric surgery. They will be randomly assigned to one of three groups: 1) MICT, 2) HIIT, or 3) a control group. The intervention will occur 2 days a week for 4 months. Outcomes will be assessed at four points: 1) one week before surgery; 2) 21 days after surgery (baseline before the exercise program); 3) 8 weeks after beginning the exercise program; and 4) one week after the end of intervention. Primary outcomes will include body composition, heart rate variability, and six-minute walk test and quality of life scores. Secondary outcomes will be maximal respiratory pressure, flowmeter, hand dynamometry, and 30-second sit-to-stand test results.Discussion: Both exercise protocols in this study were developed according to evidence-based practice. It is expected that, after 16 weeks of intervention, body composition (measured by electrical bioimpedance), cardiopulmonary function (measured by heart rate variability, maximal inspiratory pressure, maximal expiratory pressure, peak expiratory flow, handgrip strength, and the six-minute walk test) and perceived quality of life (measured by the Moorehead-Ardelt quality of life questionnaire II and bariatric analysis and reporting outcome system scores) will improve, especially in the HIIT group.Trial registration: ClinicalTrials.gov, NCT04235842, registered 22 January 2020.
Enhanced Sensitivity of VEGF Detection Using Catalase-Mediated Chemiluminescence Immunoassay Based on CdTe QD/H2O2 System
Authors: Ghavamipour Fahimeh , Rahmani Hossein , Shanehsaz Maryam , Khajeh Khosro , Mirshahi Manouchehr , Sajedi Reza
Published: June 2020

Background: Since vascular endothelial growth factor (VEGF) is a major regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of H 2 O 2 -sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran system as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme. Results: Under the optimized experimental conditions, the CL-ELISA method can detect VEGF in the excellent linear range of 2–35000 pg mL -1 , with a detection limit (S/N = 3) of 0.5 pg mL -1 which was approximately 10 times lower than the commercial colorimetric immunoassay. This proposed method has been successfully applied to the clinical determination of VEGF in the human serum samples and the results illustrated the excellent correlation with the conventional ELISA method (R 2 = 0.997). The suitable recovery rate of the method in the serum ranged from 97% to 107%, with a relative standard deviation of 1.2% to 13.4%. Conclusions: The novel immunoassay proposes a highly sensitive, specific and stable method for very low levels detection of VEGF that can be used in primary diagnosis of tumor. With the well-designed sensing platform, this approach has a broad potential to be applied for quantitative analysis of numerous disease-related protein biomarkers for which antibodies are available.
Efficacy and Safety of Traditional Chinese Medicine in COVID-2019: A Study Protocol for an Open-Label, Multicenter Randomized Controlled Trial
Authors: Zeng Congcong , Yuan Zhengzhong , Pan Xiaoqiong , Zhang Jizhou , Zhu Jiahui , Zhou Fan , Shan Zhuocheng , Yuan Ye , Ye Ren , Cheng Jinguo
Published: June 2020

Introduction: The outbreak of a novel coronavirus (SARS-CoV-2) and associated COVID-19 disease in late December 2019 has led to a global pandemic. It directly leads to high morbidity and mortality, but also results in a devastating effect on the global economy. Unfortunately, there are no effective therapies or vaccines for it. Hence, we designed a randomized trial to evaluate the efficacy and safety of Traditional Chinese Medicine for treating patients with COVID-19.Methods and analysis: This is an open-label, multicenter randomized controlled clinical trial. One hundred and twelve patients infected by SARS-CoV-2 will be randomly assigned to the experimental or the control group in an equal ratio. The patients in control group will accept routine supportive clinically care including the therapies of anti-viral, anti-bacterial and ameliorating the related symptoms, while patients in the experimental group will be asked to take traditional Chinese medicine depending on the different stages of the disease for consecutive 14 days in addition to supportive care. All data will be gathered at baseline and on days 3, 7, 10 and 14. The primary outcome measures will be the time of Reverse Transcription PCR testing of respiratory tract sample turns to be negative. Secondary outcome measures will include Murray lung injury score, MuLBSTA score and TCM ( Traditional Chinese Medicine ) Syndrome Scoring System. A laboratory test will be taken before and after treatment to assess the safety of TCM.Discussion: The study may help to identify the the efficacy and safety of Traditional Chinese Medicine in treating COVID-2019.Trial registration: Chinese Clinical Trial Registry, ChiCTR2000030759.Registered on March 13th 2020-Retrospectively registered, http://www.chictr.org.cn/.