bluuebunny/crossref_metadata_2025 huggingface dataset:
Reciprocating Thermal Behavior in Multichannel Relaxation of Cobalt(II) Based Single Ion Magnets
Authors: Rajnák Cyril , Titiš Ján , Boča Roman
Published: May 2021

A series of mononuclear Co(II) complexes showing slow magnetic relaxation is assessed from the point of view of relaxation mechanisms. In certain cases, the reciprocating thermal behavior is detected: On cooling, the slow relaxation time is prolonged until a certain limit and then, unexpectedly, is accelerated. The low-temperature magnetic data can be successfully fitted by assuming Raman and/or phonon bottleneck mechanisms of the slow magnetic relaxation for the high-frequency relaxation channel. An additional term with the negative temperature exponent is capable of reproducing the whole experimental dataset.
Amended Criteria of Oscillation for Nonlinear Functional Dynamic Equations of Second-Order
Authors: Hassan Taher S. , El-Nabulsi Rami Ahmad , Abdel Menaem Amir
Published: May 2021

In this paper, the sharp Hille-type oscillation criteria are proposed for a class of second-order nonlinear functional dynamic equations on an arbitrary time scale, by using the technique of Riccati transformation and integral averaging method. The obtained results demonstrate an improvement in Hille-type compared with the results reported in the literature. Some examples are provided to illustrate the significance of the obtained results.
Fusarins G–L with Inhibition of NO in RAW264.7 from Marine-Derived Fungus Fusarium solani 7227
Authors: Luo Guangyuan , Zheng Li , Wu Qilin , Chen Senhua , Li Jing , Liu Lan
Published: May 2021

Six new fusarin derivatives, fusarins G–L (1–6), together with five known compounds (5–11) were isolated from the marine-derived fungus Fusarium solani 7227. The structures of the new compounds were elucidated by means of comprehensive spectroscopic methods (1D and 2D NMR, HRESIMS, ECD, and ORC) and X-ray crystallography. Compounds 5–11 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide, with IC50 values ranging from 3.6 to 32.2 μM. The structure–activity relationships of the fusarins are discussed herein.
Applications of Human Amniotic Membrane for Tissue Engineering
Authors: Fénelon Mathilde , Catros Sylvain , Meyer Christophe , Fricain Jean-Christophe , Obert Laurent , Auber Frédéric , Louvrier Aurélien , Gindraux Florelle
Published: May 2021

An important component of tissue engineering (TE) is the supporting matrix upon which cells and tissues grow, also known as the scaffold. Scaffolds must easily integrate with host tissue and provide an excellent environment for cell growth and differentiation. Human amniotic membrane (hAM) is considered as a surgical waste without ethical issue, so it is a highly abundant, cost-effective, and readily available biomaterial. It has biocompatibility, low immunogenicity, adequate mechanical properties (permeability, stability, elasticity, flexibility, resorbability), and good cell adhesion. It exerts anti-inflammatory, antifibrotic, and antimutagenic properties and pain-relieving effects. It is also a source of growth factors, cytokines, and hAM cells with stem cell properties. This important source for scaffolding material has been widely studied and used in various areas of tissue repair: corneal repair, chronic wound treatment, genital reconstruction, tendon repair, microvascular reconstruction, nerve repair, and intraoral reconstruction. Depending on the targeted application, hAM has been used as a simple scaffold or seeded with various types of cells that are able to grow and differentiate. Thus, this natural biomaterial offers a wide range of applications in TE applications. Here, we review hAM properties as a biocompatible and degradable scaffold. Its use strategies (i.e., alone or combined with cells, cell seeding) and its degradation rate are also presented.
A Simplified Layer-by-Layer Model for Prediction of Residual Stress Distribution in Additively Manufactured Parts
Authors: Pant Prabhat , Sjöström Sören , Simonsson Kjell , Moverare Johan , Proper Sebastian , Hosseini Seyed , Luzin Vladimir , Peng Rulin
Published: May 2021

With the improvement in technology, additive manufacturing using metal powder has been a go-to method to produce complex-shaped components. With complex shapes being printed, the residual stresses (RS) developed during the printing process are much more difficult to control and manage, which is one of the issues seen in the field of AM. A simplified finite element-based, layer-by-layer activation approach for the prediction of residual stress is presented and applied to L-shaped samples built in two different orientations. The model was validated with residual stress distributions measured using neutron diffraction. It has been demonstrated that this simplified model can predict the trend of the residual stress distribution well inside the parts and give insight into residual stress evolution during printing with time for any area of interest. Although the stress levels predicted are higher than the measured ones, the impact of build direction on the development of RS during the building process and the final RS distributions after removing the base plate could be exploited using the model. This is important for finalizing the print orientation for a complex geometry, as the stress distribution will be different for different print orientations. This simplified tool which does not need high computational power and time can also be useful in component design to reduce the residual stresses.
Identification of Circulating Diagnostic Biomarkers for Coronary Microvascular Disease in Postmenopausal Women Using Machine-Learning Techniques
Authors: Arredondo Eve Alicia , Tunc Elif , Liu Yu-Jeh , Agrawal Saumya , Erbak Yilmaz Huriye , Emren Sadık Volkan , Akyıldız Akçay Filiz , Mainzer Luidmila , Žurauskienė Justina , Madak Erdogan Zeynep
Published: May 2021

Coronary microvascular disease (CMD) is a common form of heart disease in postmenopausal women. It is not due to plaque formation but dysfunction of microvessels that feed the heart muscle. The majority of the patients do not receive a proper diagnosis, are discharged prematurely and must go back to the hospital with persistent symptoms. Because of the lack of diagnostic biomarkers, in the current study, we focused on identifying novel circulating biomarkers of CMV that could potentially be used for developing a diagnostic test. We hypothesized that plasma metabolite composition is different for postmenopausal women with no heart disease, CAD, or CMD. A total of 70 postmenopausal women, 26 healthy individuals, 23 individuals with CMD and 21 individuals with CAD were recruited. Their full health screening and tests were completed. Basic cardiac examination, including detailed clinical history, additional disease and prescribed drugs, were noted. Electrocardiograph, transthoracic echocardiography and laboratory analysis were also obtained. Additionally, we performed full metabolite profiling of plasma samples from these individuals using gas chromatography-mass spectrometry (GC–MS) analysis, identified and classified circulating biomarkers using machine learning approaches. Stearic acid and ornithine levels were significantly higher in postmenopausal women with CMD. In contrast, valine levels were higher for women with CAD. Our research identified potential circulating plasma biomarkers of this debilitating heart disease in postmenopausal women, which will have a clinical impact on diagnostic test design in the future.
Human Cytomegalovirus Reduces Endothelin-1 Expression in Both Endothelial and Vascular Smooth Muscle Cells
Authors: Yaiw Koon-Chu , Mohammad Abdul-Aleem , Taher Chato , Cui Huanhuan Leah , Costa Helena , Kostopoulou Ourania N. , Jung Masany , Assinger Alice , Wilhelmi Vanessa , Yang Jiangning , Strååt Klas , Rahbar Afsar , Pernow John , Söderberg-Nauclér Cecilia
Published: May 2021

Human cytomegalovirus (HCMV) is an opportunistic pathogen that has been implicated in the pathogenesis of atherosclerosis. Endothelin-1 (ET-1), a potent vasoconstrictive peptide, is overexpressed and strongly associated with many vasculopathies. The main objective of this study was to investigate whether HCMV could affect ET-1 production. As such, both endothelial and smooth muscle cells, two primary cell types involved in the pathogenesis of atherosclerosis, were infected with HCMV in vitro and ET-1 mRNA and proteins were assessed by quantitative PCR assay, immunofluorescence staining and ELISA. HCMV infection significantly decreased ET-1 mRNA and secreted bioactive ET-1 levels from both cell types and promoted accumulation of the ET-1 precursor protein in infected endothelial cells. This was associated with inhibition of expression of the endothelin converting enzyme-1 (ECE-1), which cleaves the ET-1 precursor protein to mature ET-1. Ganciclovir treatment did not prevent the virus suppressive effects on ET-1 expression. Consistent with this observation we identified that the IE2-p86 protein predominantly modulated ET-1 expression. Whether the pronounced effects of HCMV in reducing ET-1 expression in vitro may lead to consequences for regulation of the vascular tone in vivo remains to be proven.
Statherian (ca. 1714–1680 Ma) Extension-Related Magmatism and Deformation in the Southwestern Korean Peninsula and Its Geological Significance: Constraints from the Petrological, Structural, Geochemical and Geochronological Studies of Newly Identified Granitoids
Authors: Lee Byung Choon , Kee Weon-Seo , Byun Uk Hwan , Kim Sung Won
Published: May 2021

In this study, petrological, structural, geochemical, and geochronological analyses of the Statherian alkali feldspar granite and porphyritic alkali feldspar granite in the southwestern part of the Korean Peninsula were conducted to examine petrogenesis of the granitoids and their tectonic setting. Zircon U-Pb dating revealed that the two granites formed around 1.71 Ga and 1.70–1.68 Ga, respectively. The results of the geochemical analyses showed that both of the granites have a high content of K2O, Nb, Ta, and Y, as well as high FeOt/MgO and Ga/Al ratios. Both granites have alkali-calcic characteristics with a ferroan composition, indicating an A-type affinity. Zircon Lu-Hf isotopic compositions yielded negative εHf(t) values (−3.5 to −10.6), indicating a derivation from ancient crustal materials. Both granite types underwent ductile deformation and exhibited a dextral sense of shear with a minor extension component. Based on field relationships and zircon U-Pb dating, it was considered that the deformation event postdated the emplacement of the alkali feldspar granite and terminated soon after the emplacement of the porphyritic alkali feldspar granite in an extensional setting. These data indicated that there were extension-related magmatic activities accompanying ductile deformation in the southwestern part of the Korean Peninsula during 1.71–1.68 Ga. The Statherian extension-related events are well correlated with those in the midwestern part of the Korean and eastern parts of the North China Craton.
Effect of Land Reclamation on Soil Properties, Mineralogy and Trace-Element Distribution and Availability: The Example of Technosols Developed on the Tailing Disposal Site of an Abandoned Zn and Pb Mine
Authors: Tarnawczyk Magdalena , Uzarowicz Łukasz , Perkowska-Pióro Katarzyna , Pędziwiatr Artur , Kwasowski Wojciech
Published: May 2021

Land reclamation is a common practice leading to the restoration of areas affected by industrial activity. Soil studies in reclaimed areas are very useful to determine the effectiveness of reclamation works. The goal of the study was to investigate soil properties, mineral composition, total concentrations of Zn, Pb, Cd and As and chemical forms of these elements in order to assess the success of land reclamation of the abandoned mine tailing disposal site of the “Trzebionka” Zn-Pb mine in Trzebinia, southern Poland. The disposal site was reclaimed by covering tailings with a layer of inert material with a thickness up to 25 cm. The topsoil of the studied soil profiles was comprised of sandy loamy/loamy materials and the subsoil was comprised of sandy tailing materials. The soils were characterized by a neutral or slightly alkaline reaction due to the high content of carbonates. The dominant mineral in the subsoil was dolomite. The studied soils were considerably contaminated with Zn, Pb, Cd and As. A high load of mobile Zn, Pb and Cd was typical of the subsoil material. The reclamation layer does not provide sufficient isolation of toxic tailings from the environment and there is still a high risk of element uptake by plants.
The Carbonate-Hosted Tullacondra Cu-Ag Deposit, Mallow, Ireland
Authors: Silva Andressa A. , Cordeiro Pedro , Johnson Sean C. , Lagoeiro Leonardo E. , Corcoran Loretta , Simonetti Antonio , Meere Patrick A. , Unitt Richard , Colaço Laisa Stingelin , Santos Anderson M.
Published: May 2021

The Tullacondra Cu-Ag deposit is located on the southern margin of the Lower Carboniferous Irish Midlands orefield and contains historical reserves of approximately 4.2 Mt at 0.7% Cu and 27.5 ppm Ag. The deposit is hosted within the hanging wall of a feeder fault, the EW-trending Tullacondra Fault, where sulfides and sulfosalts containing elevated Cu, Ag, As, and Sb deposited, whereas Zn and Pb are nearly absent. The deposition of Cu sulfides in Tullacondra took place along bedding and bedding-parallel dissolution seams, suggesting an epigenetic mineralization that formed: (a) the Transition Series-hosted mineralized zone containing elevated Cu associated with Ag, As, and Sb; (b) the Lower Limestone Shale-hosted mineralized zone, Cu-dominated and depleted in other metals, and (c) a near-vertical mineralized zone associated with fractures related to the Tullacondra Fault. Some similarities are shared with Irish-type Zn-Pb deposits, such as structural and stratigraphic controls, and elevated Cu, Ag, As, and Sb within feeder-fault proximal zones (such as in Lisheen and Silvermines). Whether Tullacondra mineralization was part of the Irish-type system or not, our deposit geometry evaluation, whole-rock geochemistry, paragenetic sequence, and texture relationships indicate that Cu-Ag deposition involved the reaction of metal-bearing fluids with carbonate rocks.
Automated Optical Image Analysis of Iron Ore Sinter
Authors: Donskoi Eugene , Hapugoda Sarath , Manuel James Robert , Poliakov Andrei , Peterson Michael John , Mali Heinrich , Bückner Birgit , Honeyands Tom , Pownceby Mark Ian
Published: May 2021

Sinter quality is a key element for stable blast furnace operation. Sinter strength and reducibility depend considerably on the mineral composition and associated textural features. During sinter optical image analysis (OIA), it is important to distinguish different morphologies of the same mineral such as primary/secondary hematite, and types of silico-ferrite of calcium and aluminum (SFCA). Standard red, green and blue (RGB) thresholding cannot effectively segment such morphologies one from another. The Commonwealth Scientific Industrial Research Organization’s (CSIRO) OIA software Mineral4/Recognition4 incorporates a unique textural identification module allowing various textures/morphologies of the same mineral to be discriminated. Together with other capabilities of the software, this feature was used for the examination of iron ore sinters where the ability to segment different types of hematite (primary versus secondary), different morphological sub-types of SFCA (platy and prismatic), and other common sinter phases such as magnetite, larnite, glass and remnant aluminosilicates is crucial for quantifying sinter petrology. Three different sinter samples were examined. Visual comparison showed very high correlation between manual and automated phase identification. The OIA results also gave high correlations with manual point counting, X-ray Diffraction (XRD) and X-ray Fluorescence (XRF) analysis results. Sinter textural classification performed by Recognition4 showed a high potential for deep understanding of sinter properties and the changes of such properties under different sintering conditions.
Application of 3D Electrical Resistivity Tomography in the Yeoncheon Titanomagnetite Deposit, South Korea
Authors: Shin Youngjae , Shin Seungwook , Cho Seong-Jun , Son Jeong-Sul
Published: May 2021

The Yeoncheon titanomagnetite deposit formed by Precambrian magma differentiation is located in Gyeonggi-do, South Korea. Our team conducted an airborne magnetic survey for multiscale mineral exploration and then selected a promising survey area. An electrical resistivity survey was carried out in the potential area to image subsurface structure. Because ore minerals are mainly distributed in gabbro monzodiorite rather than quartz monzodiorite, we applied three-dimensional inversion of electrical resistivity tomography (ERT) data to identify lithology boundaries related to magma differentiation. The resistivity criterion distinguishing the lithologies of gabbro and quartz monzodiorite was determined from laboratory resistivity experimental results performed on drilling cores. The selected region for gabbro monzodiorite extends to the northeast direction, which is consistent with the geology map, magnetic anomaly, and drilling data. The inversion results of ERT can help in selecting the location of geophysical survey or drilling.
Lycopene Inhibits Toll-Like Receptor 4-Mediated Expression of Inflammatory Cytokines in House Dust Mite-Stimulated Respiratory Epithelial Cells
Authors: Choi Jiyeon , Lim Joo Weon , Kim Hyeyoung
Published: May 2021

House dust mites (HDM) are critical factors in airway inflammation. They activate respiratory epithelial cells to produce reactive oxygen species (ROS) and activate Toll-like receptor 4 (TLR4). ROS induce the expression of inflammatory cytokines in respiratory epithelial cells. Lycopene is a potent antioxidant nutrient with anti-inflammatory activity. The present study aimed to investigate whether HDM induce intracellular and mitochondrial ROS production, TLR4 activation, and pro-inflammatory cytokine expression (IL-6 and IL-8) in respiratory epithelial A549 cells. Additionally, we examined whether lycopene inhibits HDM-induced alterations in A549 cells. The treatment of A549 cells with HDM activated TLR4, induced the expression of IL-6 and IL-8, and increased intracellular and mitochondrial ROS levels. TAK242, a TLR4 inhibitor, suppressed both HDM-induced ROS production and cytokine expression. Furthermore, lycopene inhibited the HDM-induced TLR4 activation and cytokine expression, along with reducing the intracellular and mitochondrial ROS levels in HDM-treated cells. These results collectively indicated that the HDM induced TLR4 activation and increased intracellular and mitochondrial ROS levels, thus resulting in the induction of cytokine expression in respiratory epithelial cells. The antioxidant lycopene could inhibit HDM-induced cytokine expression, possibly by suppressing TLR4 activation and reducing the intracellular and mitochondrial ROS levels in respiratory epithelial cells.
β-Sitosterol-D-Glucopyranoside Mimics Estrogenic Properties and Stimulates Glucose Utilization in Skeletal Muscle Cells
Authors: Pandey Jyotsana , Dev Kapil , Chattopadhyay Sourav , Kadan Sleman , Sharma Tanuj , Maurya Rakesh , Sanyal Sabyasachi , Siddiqi Mohammad Imran , Zaid Hilal , Tamrakar Akhilesh Kumar
Published: May 2021

Estrogenic molecules have been reported to regulate glucose homeostasis and may be beneficial for diabetes management. Here, we investigated the estrogenic effect of β-sitosterol-3-O-D-glucopyranoside (BSD), isolated from the fruits of Cupressus sempervirens and monitored its ability to regulate glucose utilization in skeletal muscle cells. BSD stimulated ERE-mediated luciferase activity in both ERα and ERβ-ERE luc expression system with greater response through ERβ in HEK-293T cells, and induced the expression of estrogen-regulated genes in estrogen responsive MCF-7 cells. In silico docking and molecular interaction studies revealed the affinity and interaction of BSD with ERβ through hydrophobic interaction and hydrogen bond pairing. Furthermore, prolonged exposure of L6-GLUT4myc myotubes to BSD raised the glucose uptake under basal conditions without affecting the insulin-stimulated glucose uptake, the effect associated with enhanced translocation of GLUT4 to the cell periphery. The BSD-mediated biological response to increase GLUT4 translocation was obliterated by PI-3-K inhibitor wortmannin, and BSD significantly increased the phosphorylation of AKT (Ser-473). Moreover, BSD-induced GLUT4 translocation was prevented in the presence of fulvestrant. Our findings reveal the estrogenic activity of BSD to stimulate glucose utilization in skeletal muscle cells via PI-3K/AKT-dependent mechanism.
Oxidative Stress in Down and Williams-Beuren Syndromes: An Overview
Authors: Ferrari Marta , Stagi Stefano
Published: May 2021

Oxidative stress is the result of an imbalance in the redox state in a cell or a tissue. When the production of free radicals, which are physiologically essential for signaling, exceeds the antioxidant capability, pathological outcomes including oxidative damage to macromolecules, aberrant signaling, and inflammation can occur. Down syndrome (DS) and Williams-Beuren syndrome (WBS) are well-known and common genetic conditions with multi-systemic involvement. Their etiology is linked to oxidative stress with important causative genes, such as SOD-1 and NCF-1, respectively, of the diseases being primarily involved in the regulation of the redox state. Early aging, dementia, autoimmunity, and chronic inflammation are some of the main characteristics of these conditions that can be associated with oxidative stress. In recent decades, there has been a growing interest in the possible role of oxidative stress and inflammation in the pathology of these conditions. However, at present, few studies have investigated these correlations. We provide an overview of the current literature concerning the role of oxidative stress and oxidative damage in genetic syndromes with a focus on Down syndrome and WBS. We hope to provide new insights to improve the management of complications related to these diseases.
Pharmacological Insights into Halophyte Bioactive Extract Action on Anti-Inflammatory, Pain Relief and Antibiotics-Type Mechanisms
Authors: Giordano Rocco , Saii Zeinab , Fredsgaard Malthe , Hulkko Laura Sini Sofia , Poulsen Thomas Bouet Guldbæk , Thomsen Mikkel Eggert , Henneberg Nanna , Zucolotto Silvana Maria , Arendt-Nielsen Lars , Papenbrock Jutta , Thomsen Mette Hedegaard , Stensballe Allan
Published: May 2021

The pharmacological activities in bioactive plant extracts play an increasing role in sustainable resources for valorization and biomedical applications. Bioactive phytochemicals, including natural compounds, secondary metabolites and their derivatives, have attracted significant attention for use in both medicinal products and cosmetic products. Our review highlights the pharmacological mode-of-action and current biomedical applications of key bioactive compounds applied as anti-inflammatory, bactericidal with antibiotics effects, and pain relief purposes in controlled clinical studies or preclinical studies. In this systematic review, the availability of bioactive compounds from several salt-tolerant plant species, mainly focusing on the three promising species Aster tripolium, Crithmum maritimum and Salicornia europaea, are summarized and discussed. All three of them have been widely used in natural folk medicines and are now in the focus for future nutraceutical and pharmacological applications.
C-11, a New Antiepileptic Drug Candidate: Evaluation of the Physicochemical Properties and Impact on the Protective Action of Selected Antiepileptic Drugs in the Mouse Maximal Electroshock-Induced Seizure Model
Authors: Zagaja Mirosław , Szewczyk Aleksandra , Szala-Rycaj Joanna , Raszewski Grzegorz , Chrościńska-Krawczyk Magdalena , Abram Michał , Kamiński Krzysztof , Andres-Mach Marta
Published: May 2021

C-11 is a hybrid compound derived from 2-(2,5-dioxopyrrolidin-1-yl) propanamide, with a wide spectrum of anticonvulsant activity and low neurotoxicity. The aim of this study was to determine the effects of C-11 on the protective action of various antiepileptic drugs (i.e., carbamazepine CBZ, lacosamide LCM, lamotrigine LTG, and valproate VPA) against maximal electroshock-induced seizures (MES) in mice, as well as its neuroprotective and physicochemical/pharmacokinetic properties. Results indicate that C-11 (30 mg/kg, i.p.) significantly enhanced the anticonvulsant action of LCM (p < 0.001) and VPA (p < 0.05) but not that of CBZ and LTG in the MES test. Neither C-11 (30 mg/kg) alone nor its combination with other anticonvulsant drugs (at their ED50 values from the MES test) affected motor coordination; skeletal muscular strength and long-term memory, as determined in the chimney; grip strength and passive avoidance tests, respectively. Pharmacokinetic characterization revealed that C-11 had no impact on total brain concentrations of LCM or VPA in mice. Qualitative analysis of neuroprotective properties of C-11, after a single administration of pilocarpine, revealed no protective effect of this substance in the tested animals. Determination of physicochemical descriptors showed that C-11 meets the drug-likeness requirements resulting from Lipinski and Veber’s rules and prediction of gastrointestinal absorption and brain penetration, which is extremely important for the CNS-active compounds.
Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants
Authors: Kasperkiewicz Katarzyna , Major Roman , Sypien Anna , Kot Marcin , Dyner Marcin , Major Łukasz , Byrski Adam , Kopernik Magdalena , Lackner Juergen M.
Published: May 2021

The goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy focused both on improvement of antibacterial properties against bacteria adhesion and on wear and corrosion resistance of tools for spine surgery. Further, a ~35% decrease in implant and tool dimensions was expected by introducing ultrahigh-strength titanium alloys for less-invasive surgeries. The tested materials, in the form of thin, multi-layered coatings, showed nanocrystalline microstructures. Performed direct-cytotoxicity studies (including lactate dehydrogenase activity measurement) showed that there was a low probability of adverse effects on surrounding SAOS-2 (Homo sapiens bone osteosarcoma) cells. The microbiological studies (e.g., ISO 22196 contact tests) showed that implanting Ag nanoparticles into Ti/TixN coatings inhibited the growth of E. coli and S. aureus cells and reduced their adhesion to the material surface. These findings suggest that Ag-nanoparticles present in implant coatings may potentially minimize infection risk and lower inherent stress.
A Systematic Literature Review on the User Experience Design for Game-Based Interventions via 3D Virtual Worlds in K-12 Education
Authors: Pellas Nikolaos , Mystakidis Stylianos , Christopoulos Athanasios
Published: May 2021

A substantial body of literature has well-documented and demonstrated the potential of using three-dimensional (3D) virtual worlds (VWs) across various learning subjects and contexts in primary and secondary (K-12) education. However, little is known when it comes to issues related to child-interaction research and the impact that design decisions have on the user experience (UX), especially when game-based learning approaches are employed in 3DVWs. Hence, in this systematic literature review, we appraise and summarize the most relevant research articles (n = 30) conducted in K-12 settings, published between 2006–2020 and that elicit information related to (a) the interaction design (ID) of game events and trends associated with game elements and features that were utilized for the development and creation of game prototypes, (b) the research methods which were followed to empirically evaluate their teaching interventions, and (c) the design-related issues and factors affecting ID and UX by identifying the most frequent set of learning and game mechanics that were adopted in various game prototypes in different learning subjects. The vast majority of game prototypes enhanced students’ engagement and participation, affecting their achievements positively. This systematic literature review provides clear guidelines regarding the design decisions that educational stakeholders should consider, and provides recommendations on how to assess and evaluate the students’ learning experience (i.e., performance, achievements, outcomes) using 3DVWs.
Effect of Solvent on Fluorescence Emission from Polyethylene Glycol-Coated Graphene Quantum Dots under Blue Light Illumination
Authors: Yang Po-Chih , Ting Yu-Xuan , Gu Siyong , Ashraf Gandomi Yasser , Li Jianlin , Hsieh Chien-Te
Published: May 2021

To explore aggregate-induced emission (AIE) properties, this study adopts a one-pot hydrothermal route for synthesizing polyethylene glycol (PEG)-coated graphene quantum dot (GQD) clusters, enabling the emission of highly intense photoluminescence under blue light illumination. The hydrothermal synthesis was performed at 300 °C using o-phenylenediamine as the nitrogen and carbon sources in the presence of PEG. Three different solvents, propylene glycol methyl ether acetate (PGMEA), ethanol, and water, were used for dispersing the PEG-coated GQDs, where extremely high fluorescent emission was achieved at 530–550 nm. It was shown that the quantum yield (QY) of PEG-coated GQD suspensions is strongly dependent on the solvent type. The pristine GQD suspension tends to be quenched (i.e., QY: ~1%) when dispersed in PGMEA (aggregation-caused quenching). However, coating GQD nanoparticles with polyethylene glycol results in substantial enhancement of the quantum yield. When investigating the photoluminescence emission from PEG-coated GQD clusters, the surface tension of the solvents was within the range of from 26.9 to 46.0 mN/m. This critical index can be tuned for assessing the transition point needed to activate the AIE mechanism which ultimately boosts the fluorescence intensity. The one-pot hydrothermal route established in this study can be adopted to engineer PEG-coated GQD clusters with solid-state PL emission capabilities, which are needed for next-generation optical, bio-sensing, and energy storage/conversion devices.
On the Development of an Effective Method to Produce Conductive PCL Film
Authors: Damonte Giacomo , Vallin Alberto , Fina Alberto , Monticelli Orietta
Published: May 2021

The aim of this work was to develop an effective approach to improve the graphite dispersion and, consequently, the electrical conductivity of nanocomposites based on polycaprolactone (PCL) and graphite nanoplates (GNP). With this aim, a polymeric additive was designed to be compatible with the polymer matrix and capable of interacting with the graphite layers. Indeed, the compound consists of a low molecular mass PCL ending with a pyrene group (Pyr-PCL). The exploitation of such a molecule is expected to promote from one side specific interactions of the pyrene terminal group with the surface of graphite layers and from the other to guarantee the compatibility with PCL, having a chain with the same nature as the matrix. The features of the nanocomposites prepared by directly blending PCL with GNP were compared with those of the same systems also containing the additive. Moreover, a neat mixture, based on PCL and PCL-Pyr, was prepared and characterized. The specific interactions between the ad hoc synthesized compound and graphite were verified by UV measurements, while SEM characterization demonstrated a finer dispersion of GNP in the samples containing Pyr-PCL. GNP nucleating effect, proved by the increase in the crystallization temperature, was observed in all the samples containing the nanofiller. Moreover, a significant improvement of the electrical conductivity was found in the systems based on the pyrenyl terminated PCL. This peculiar and interesting phenomenon was related to the optimized nanofiller dispersion and to the ameliorated compatibility with the polymer matrix.
The Use of Bovine Colostrum in Sport and Exercise
Authors: Davison Glen
Published: May 2021

There has been a great deal of interest in bovine colostrum within sports nutrition over the last 25 years. Studies have investigated the effects on body composition, physical performance, recovery, gut damage and permeability, immune function, and illness risk. This narrative review considers available evidence in each of these areas. Although some studies have shown protection against performance decrements caused by periods of intensified training, there is limited evidence for effects on body composition and physical performance. There is stronger evidence for benefit on gut permeability and damage markers and on immune function and illness risk, especially during periods of intensified training. The balance of available evidence for gut permeability and illness risk is positive, but further research is required to fully determine all mechanisms responsible for these effects. Early suggestions that supplementation with bovine colostrum products could increase systemic IGF-1 levels are not supported by the balance of available evidence examining a range of doses over both short- and long-term periods. Nevertheless, dose–response studies would be valuable for determining the minimum efficacious dose, although this is complicated by variability in bioactivity between products, making any dose–response findings applicable only to the specific products used in such studies.
Application of Microbiome Management in Therapy for Clostridioides difficile Infections: From Fecal Microbiota Transplantation to Probiotics to Microbiota-Preserving Antimicrobial Agents
Authors: Chiu Chun-Wei , Tsai Pei-Jane , Lee Ching-Chi , Ko Wen-Chien , Hung Yuan-Pin
Published: May 2021

Oral vancomycin and metronidazole, though they are the therapeutic choice for Clostridioides difficile infections (CDIs), also markedly disturb microbiota, leading to a prolonged loss of colonization resistance to C. difficile after therapy; as a result, their use is associated with a high treatment failure rate and high recurrent rate. An alternative for CDIs therapy contains the delivery of beneficial (probiotic) microorganisms into the intestinal tract to restore the microbial balance. Recently, mixture regimens containing Lactobacillus species, Saccharomyces boulardii, or Clostridium butyricum have been extensively studied for the prophylaxis of CDIs. Fecal microbiota transplantation (FMT), the transfer of (processed) fecal material from healthy donors to patients for treating CDIs, combined with vancomycin was recommended as the primary therapy for multiple recurrent CDIs (rCDIs). Either probiotics or FMT have been utilized extensively in preventing or treating CDIs, aiming at less disturbance in the microbiota to prevent rCDIs after therapy cessation. Otherwise, many newly developed therapeutic agents have been developed and aim to preserve microbiota during CDI treatment to prevent disease recurrence and might be useful in clinical patients with rCDIs in the future.
Effects of Theileria orientalis Infection on Health Status and Productivity of Dairy Cows Reared inside Barns
Authors: Fukushima Yuki , Minamino Tomoya , Mikurino Yoko , Honkawa Kazuyuki , Horii Yoichiro , Taniguchi Takako , Mekata Hirohisa , Sasaki Yosuke
Published: May 2021

The objective of the present study was to investigate the effects of Theileria orientalis on the severity of anemia, the prevalence of disease within 21 days after calving and productivity in cows raised inside barns. This longitudinal observational study, which was conducted on a commercial dairy farm in Japan, involved 627 Holstein cows subjected to PCR analysis for T. orientalis. In study 1, we collected blood samples from 156 sick cows within 21 days after calving, and we found the prevalence of T. orientalis infection to be 65.4%. In study 2, we randomly selected 471 cows during the dry period and collected blood samples to conduct PCR analysis for T. orientalis and determined the prevalence of T. orientalis infection to be 69.0%. Compared with the values for the T. orientalis-uninfected group, the T. orientalis-infected cows had significantly decreased hemoglobin concentrations and hematocrit, but there were no differences in the other complete blood count indexes between the two groups. In addition, there were no differences in productivity and the prevalence of major diseases between the T. orientalis-infected and uninfected cows. In summary, T. orientalis had few effects on anemia, productivity and the health of cows raised inside a barn.
RETRACTED: Lipidic Nano-Sized Emulsomes Potentiates the Cytotoxic and Apoptotic Effects of Raloxifene Hydrochloride in MCF-7 Human Breast Cancer Cells: Factorial Analysis and In Vitro Anti-Tumor Activity Assessment
Authors: Aldawsari Hibah , Ahmed Osama , Alhakamy Nabil , Neamatallah Thikryat , Fahmy Usama , Badr-Eldin Shaimaa
Published: May 2021

Raloxifene hydrochloride (RLX), an antiosteoporotic agent, has been utilized for guarding against breast cancer and recently, for the disease management owing to its estrogen antagonist activity. Nevertheless, RLX exhibits poor bioavailability that could be attributed to reduced water solubility and first pass metabolism. To overcome these challenges, this study aimed at formulating and optimizing RLX emulsomes (RLX-EMLs) to enhance the drug antitumor activity. A 4131 factorial design was employed for assessing the effect of lipoid: solid lipid ratio and solid lipid type on the emulsomes characteristics. The anticancer potential of the optimized formulation and apoptotic parameters were assessed. Vesicle size, entrapment, and release efficiency were significantly influenced by both variables, while zeta potential was influenced by lipoid: solid lipid at p < 0.05. The optimal formulation exhibited vesicle size of 236 ± 8.6 nm, zeta potential of −18.6 ± 0.7 mV, drug entrapment of 98.9 ± 4.9%, and release efficiency of 42.7 ± 1.8%. MTT assay showed concentration-dependent inhibition of MCF-7 cells viability. In addition, cells treated with RLX-EMLs showed significant arrest at G2/M phase associated with significant increase in apoptotic and necrotic cells. The enhanced cytotoxic and anti-proliferative effect of RLX-EMLs relative to raw drug was authenticated through increased Bax/Bcl-2 ratio, caspase-9 activation and depletion of mitochondrial membrane potential.
Polishing the Therapy of Onychomycosis Induced by Candida spp.: Amphotericin B–Loaded Nail Lacquer
Authors: Souza Aleph M. S. , Ribeiro Renato C. A. , Pinheiro Gleyse K. L. O. , Pinheiro Francisco I. , Oliveira Wógenes N. , Souza Luanda B. F. C. , Silva André L. , Amaral-Machado Lucas , Alencar Éverton N. , Chaves Guilherme M. , Egito Eryvaldo S. T.
Published: May 2021

Onychomycosis induced by Candida spp. has several limitations regarding its treatment. Nail lacquers display the potential to overcome these drawbacks by providing therapeutic compliance and increasing local drug bioavailability. Thus, this work aimed to produce a nail lacquer loaded with Amphotericin B (AmB) and evaluate its performance. The AmB-loaded nail lacquer was produced and preliminarily characterized. An AmB quantification method was developed. Stability, drug release, permeability and anti-Candida activity assays were conducted. The analytical method validation met the acceptance criteria. The drug loading efficiency was 100% (0.02 mg/g of total product), whereas the AmB stability was limited to ≅7 days (≅90% remaining). The nail lacquer displayed a drying time of 187 s, non-volatile content of around 20%w/w, water-resistance of approximately 2%w/w of weight loss and satisfactory in vitro adhesion. Moreover, the in vitro antifungal activity against different Candida spp. strains was confirmed. The AmB release and the ex vivo permeability studies revealed that AmB leaves the lacquer and permeates the nail matrix in 47.76 ± 0.07% over 24 h. In conclusion, AmB-loaded nail lacquer shows itself as a promising extemporaneous dosage form with remarkable anti-Candida activity related to onychomycosis.
Biomimetic Nanotechnology: A Natural Path Forward for Tumor-Selective and Tumor-Specific NIR Activable Photonanomedicines
Authors: Prajapati Sushant , Hinchliffe Taylor , Roy Vinay , Shah Nimit , Jones Caroline N. , Obaid Girgis
Published: May 2021

The emergence of biomimetic nanotechnology has seen an exponential rise over the past decade with applications in regenerative medicine, immunotherapy and drug delivery. In the context of nanomedicines activated by near infrared (NIR) photodynamic processes (photonanomedicines; PNMs), biomimetic nanotechnology is pushing the boundaries of activatable tumor targeted nanoscale drug delivery systems. This review discusses how, by harnessing a unique collective of biological processes critical to targeting of solid tumors, biomimetic PNMs (bPNMs) can impart tumor cell specific and tumor selective photodynamic therapy-based combination regimens. Through molecular immune evasion and self-recognition, bPNMs can confer both tumor selectivity (preferential bulk tumor accumulation) and tumor specificity (discrete molecular affinity for cancer cells), respectively. They do so in a manner that is akin, yet arguably superior, to synthetic molecular-targeted PNMs. A particular emphasis is made on how bPNMs can be engineered to circumvent tumor cell heterogeneity, which is considered the Achilles’ heel of molecular targeted therapeutics. Forward-looking propositions are also presented on how patient tumor heterogeneity can ultimately be recapitulated to fabricate patient-specific, heterogeneity-targeting bPNMs.
Climate Change, Crop Yields, and Grain Quality of C3 Cereals: A Meta-Analysis of [CO2], Temperature, and Drought Effects
Authors: Ben Mariem Sinda , Soba David , Zhou Bangwei , Loladze Irakli , Morales Fermín , Aranjuelo Iker
Published: May 2021

Cereal yield and grain quality may be impaired by environmental factors associated with climate change. Major factors, including elevated CO2 concentration ([CO2]), elevated temperature, and drought stress, have been identified as affecting C3 crop production and quality. A meta-analysis of existing literature was performed to study the impact of these three environmental factors on the yield and nutritional traits of C3 cereals. Elevated [CO2] stimulates grain production (through larger grain numbers) and starch accumulation but negatively affects nutritional traits such as protein and mineral content. In contrast to [CO2], increased temperature and drought cause significant grain yield loss, with stronger effects observed from the latter. Elevated temperature decreases grain yield by decreasing the thousand grain weight (TGW). Nutritional quality is also negatively influenced by the changing climate, which will impact human health. Similar to drought, heat stress decreases starch content but increases grain protein and mineral concentrations. Despite the positive effect of elevated [CO2], increases to grain yield seem to be counterbalanced by heat and drought stress. Regarding grain nutritional value and within the three environmental factors, the increase in [CO2] is possibly the more detrimental to face because it will affect cereal quality independently of the region.
Selective Anticancer Properties, Proapoptotic and Antibacterial Potential of Three Asplenium Species
Authors: Petkov Venelin , Batsalova Tsvetelina , Stoyanov Plamen , Mladenova Tsvetelina , Kolchakova Desislava , Argirova Mariana , Raycheva Tsvetanka , Dzhambazov Balik
Published: May 2021

The ferns Asplenium ceterach L., Asplenium scolopendrium L. and Asplenium trichomanes L. have wide application in traditional medicine worldwide. However, the scientific research on their anticancer and antibacterial properties is insufficient. The present article aims to provide more information on this topic. Extracts derived from the aerial parts of A. ceterach, A. scolopendrium and A. trichomanes were examined using a panel of in vitro assays with different bacterial and mammalian cells. The cytotoxicity and anticancer activity of the samples were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Trypan blue assays with three human (A549, FL, HeLa) and three murine (3T3, TIB-71, LS48) cell lines. Inhibitory effects on the growth of Gram-positive (Bacillus cereus) and Gram-negative (Pseudomonas aeruginosa) bacteria were determined by the agar diffusion assay. Apoptosis-inducing properties of the extracts were analyzed by flow cytometry. Superoxide dismutase (SOD) activity in extract-treated cells was investigated by ELISA. The obtained results demonstrate selective anticancer activity of all three Asplenium species. The extract from A. ceterach displayed the strongest inhibitory properties against human cervical cancer cells and bacterial cells. It induced a lower level of cytotoxicity against mouse cell lines, indicating a species-specific effect. The extract from A. trichomanes demonstrated better anticancer and antibacterial properties than the sample from A. scolopendrium. Further experiments linked the mechanism of action of A. ceterach extract with oxidative stress-inducing potential and strong proapoptotic potential against the cervical cancer cell line HeLa. A. trichomanes and A. scolopendrium extracts appeared to be potent inducers of necrotic cell death.
Polymer Composites Filled with Metal Derivatives: A Review of Flame Retardants
Authors: Ilyas R. A. , Sapuan S. M. , Asyraf M. R. M. , Dayana D. A. Z. N. , Amelia J. J. N. , Rani M. S. A. , Norrrahim Mohd Nor Faiz , Nurazzi N. M. , Aisyah H. A. , Sharma Shubham , Ishak M. R. , Rafidah M. , Razman M. R.
Published: May 2021

Polymer composites filled with metal derivatives have been widely used in recent years, particularly as flame retardants, due to their superior characteristics, including high thermal behavior, low environmental degradation, and good fire resistance. The hybridization of metal and polymer composites produces various favorable properties, making them ideal materials for various advanced applications. The fire resistance performance of polymer composites can be enhanced by increasing the combustion capability of composite materials through the inclusion of metallic fireproof materials to protect the composites. The final properties of the metal-filled thermoplastic composites depend on several factors, including pore shape and distribution and morphology of metal particles. For example, fire safety equipment uses polyester thermoplastic and antimony sources with halogenated additives. The use of metals as additives in composites has captured the attention of researchers worldwide due to safety concern in consideration of people’s life and public properties. This review establishes the state-of-art flame resistance properties of metals/polymer composites for numerous industrial applications.