bluuebunny/crossref_metadata_2025 huggingface dataset:
Enzyme and Antioxidant Activities of Malted Bambara Groundnut as Affected by Steeping and Sprouting Times
Authors: Adetokunboh Adeola Helen , Obilana Anthony O. , Jideani Victoria A.
Published: March 2022

Bambara groundnut (BGN) is termed a complete food due to its nutritional composition and has been researched often for its nutritional constituents. Malting BGN seeds have shown improved nutritional and functional characteristics, which can be used to produce an amylase-rich product as a functional ingredient for food and beverage production in homes and industries. The aim of this study was to investigate the enzyme and antioxidant activities of malted BGN affected by steeping and sprouting times. BGN was malted by steeping in distilled water at 25–30 °C for 36 and 48 h and then sprouted for 144 h at 30 °C. Samples were drawn every 24 h for drying to study the effect of steeping and sprouting times on the moisture, sprout length, pH, colour, protein content, amylase, total polyphenols, and antioxidant activities of the BGN seeds. The steeping and sprouting times significantly affected the BGN malt colour quality and pH. The protein content of the malted BGN seeds was not significantly different based on steeping and sprouting times. Steeping and sprouting times significantly affected the α- and β-amylase activities of the BGN seeds. The activity of amylases for 36 and 48 h steeping times were 0.16 and 0.15 CU/g for α-amylase and were 0.22 and 0.23 BU/g for β-amylase, respectively. Amylase-rich BGN malt was produced by steeping for 36 h and sprouting for 96 h. Amylase-rich BGN malt can be useful as a functional food ingredient in food and beverage formulations.
Assessment of Drying Kinetics, Textural and Aroma Attributes of Mentha haplocalyx Leaves during the Hot Air Thin-Layer Drying Process
Authors: Guo Hui-Ling , Chen Ying , Xu Wei , Xu Meng-Tian , Sun Yong , Wang Xue-Cheng , Wang Xiao-Ya , Luo Jing , Zhang Hua , Xiong Yao-Kun
Published: March 2022

Since Mentha haplocalyx leaves are rich in bioactive constitutes, particularly volatile compounds, there are higher demands for high-quality dried medicinal and aromatic peppermint products. This study aimed to assess the drying kinetics of hot air thin layer drying Mentha haplocalyx leaves and exploring the effects of hot air-drying temperatures on the textural properties and sensory quality. According to our results, the Midilli model is the best model representing the hot air-drying process. The effective moisture diffusivity (Deff) and activation energy (Ea) of the hot air-drying process were determined as 7.51 × 10−9–3.03 × 10−8 m2/s and 57.98 KJ/moL, respectively. The changes of textural and aromatic profiles of dried Mentha haplocalyx leaves were subsequently evaluated by the SEM, GC–MS and E-nose technology. Changes in leaf cellular membrane structures were observed in this study, indicating that the loss of moisture content induced the shrinkage of leaf cells during the hot air-drying process. Moreover, the altered profile of volatile compounds was identified at the different drying temperatures. As a result of the GC-MS analysis, increasing the content of D-carvone from 61.89%, 69.25% and 78.2% resulted in drying temperatures of 35 °C, 45 °C and 55 °C, respectively; while a decreasing trend of other volatile compounds, including D-Limonene, cineole and l-caryophyllene was detected as drying temperature elevated. Finally, the aromatic profile was evaluated by E-nose, and results of the flavor radar fingerprint and PCA showed that aromatic profiles were significantly altered by the drying process. The overall results elucidated that the hot air thin layer drying at 35 °C efficiently improved the final quality of dried Mentha haplocalyx leaves by maintaining flavor properties.
Division of Labor by the HELQ, BLM, and FANCM Helicases during Homologous Recombination Repair in Drosophila melanogaster
Authors: Thomas Adam , Cox Julie , Wolfe Kelly B. , Mingalone Carrie Hui , Yaspan Haleigh R. , McVey Mitch
Published: March 2022

Repair of DNA double-strand breaks by homologous recombination (HR) requires a carefully orchestrated sequence of events involving many proteins. One type of HR, synthesis-dependent strand annealing (SDSA), proceeds via the formation of a displacement loop (D-loop) when RAD51-coated single-stranded DNA invades a homologous template. The 3′ end of the single-stranded DNA is extended by DNA synthesis. In SDSA, the D-loop is then disassembled prior to strand annealing. While many helicases can unwind D-loops in vitro, how their action is choreographed in vivo remains to be determined. To clarify the roles of various DNA helicases during SDSA, we used a double-strand gap repair assay to study the outcomes of homologous recombination repair in Drosophila melanogaster lacking the BLM, HELQ, and FANCM helicases. We found that the absence of any of these three helicases impairs gap repair. In addition, flies lacking both BLM and HELQ or HELQ and FANCM had more severe SDSA defects than the corresponding single mutants. In the absence of BLM, a large percentage of repair events were accompanied by flanking deletions. Strikingly, these deletions were mostly abolished in the blm helq and blm fancm double mutants. Our results suggest that the BLM, HELQ, and FANCM helicases play distinct roles during SDSA, with HELQ and FANCM acting early to promote the formation of recombination intermediates that are then processed by BLM to prevent repair by deletion-prone mechanisms.
Does the Expression and Epigenetics of Genes Involved in Monogenic Forms of Parkinson’s Disease Influence Sporadic Forms?
Authors: Lanore Aymeric , Lesage Suzanne , Mariani Louise-Laure , Menon Poornima Jayadev , Ravassard Philippe , Cheval Helene , Corti Olga , Brice Alexis , Corvol Jean-Christophe
Published: March 2022

Parkinson’s disease (PD) is a disorder characterized by a triad of motor symptoms (akinesia, rigidity, resting tremor) related to loss of dopaminergic neurons mainly in the Substantia nigra pars compacta. Diagnosis is often made after a substantial loss of neurons has already occurred, and while dopamine replacement therapies improve symptoms, they do not modify the course of the disease. Although some biological mechanisms involved in the disease have been identified, such as oxidative stress and accumulation of misfolded proteins, they do not explain entirely PD pathophysiology, and a need for a better understanding remains. Neurodegenerative diseases, including PD, appear to be the result of complex interactions between genetic and environmental factors. The latter can alter gene expression by causing epigenetic changes, such as DNA methylation, post-translational modification of histones and non-coding RNAs. Regulation of genes responsible for monogenic forms of PD may be involved in sporadic PD. This review will focus on the epigenetic mechanisms regulating their expression, since these are the genes for which we currently have the most information available. Despite technical challenges, epigenetic epidemiology offers new insights on revealing altered biological pathways and identifying predictive biomarkers for the onset and progression of PD.
Functional Similarities of Protein-Coding Genes in Topologically Associating Domains and Spatially-Proximate Genomic Regions
Authors: Zhao Chenguang , Liu Tong , Wang Zheng
Published: March 2022

Topologically associating domains (TADs) are the structural and functional units of the genome. However, the functions of protein-coding genes existing in the same or different TADs have not been fully investigated. We compared the functional similarities of protein-coding genes existing in the same TAD and between different TADs, and also in the same gap region (the region between two consecutive TADs) and between different gap regions. We found that the protein-coding genes from the same TAD or gap region are more likely to share similar protein functions, and this trend is more obvious with TADs than the gap regions. We further created two types of gene–gene spatial interaction networks: the first type is based on Hi-C contacts, whereas the second type is based on both Hi-C contacts and the relationship of being in the same TAD. A graph auto-encoder was applied to learn the network topology, reconstruct the two types of networks, and predict the functions of the central genes/nodes based on the functions of the neighboring genes/nodes. It was found that better performance was achieved with the second type of network. Furthermore, we detected long-range spatially-interactive regions based on Hi-C contacts and calculated the functional similarities of the gene pairs from these regions.
Challenges Facing Undergraduate Medical Education in Ambulatory Care Clinics at Tertiary Care Hospitals
Authors: Almushait Youssef B. , Alabdaljabar Mohamad S. , Alkhani Khalid , Abdalla Hesham M. , Alhayaza Raid , Temsah Mohamad-Hani , Alsohaibani Fahad
Published: March 2022

Background: Medical education has been rapidly growing and transforming due to the enormous evolution of medicine. There have been many proficient ways to learn in medicine, but academic lectures, attending wards, and ambulatory care clinics (ACC) remain the three main ways of gaining clinical knowledge and experience for medical students. Over the past decade, there has been a dramatic shift in care by focusing on ambulatory care rather than inpatient settings, which provides a golden opportunity to reinforce medical education. Purpose: Most of the published studies that have focused on the teaching barriers in ACC were descriptive rather than analytic studies. Herein, we aim to detect and determine the barriers to teaching in ACC settings using qualitative analysis. Methods: This is a cross-sectional, observational study, involving medical students in their clerkship years (i.e., fourth and fifth) from two different medical colleges in Riyadh, Saudi Arabia. Faculty who are involved in undergraduate medical education in both colleges were also included. Main Results: A total of 387 medical students studying at the two universities were enrolled in the study. Most of the participants preferred attending outpatient clinics with consultants (44.2%) and the majority preferred attending internal medicine (IM) and IM subspecialties clinics (40.4%). Regarding the challenges, students believe the top three barriers are related to: faculty (39%), environment (34.8%), and patients (14.8%). Faculty on the other hand see that the top three barriers are related to environment (55.6%), patients (24.4%), and faculty (20%). Conclusion: Undergraduate medical education in outpatient settings has many challenges. In our study, the most significant challenges were COVID-19-related restrictions, patient refusal, and insufficient time for teaching. Future studies are needed to investigate these barriers and explore potential solutions that can decrease their burden on undergraduate medical education.
Mangrove Conservation in Macao SAR, China: The Role of Environmental Education among School Students
Authors: Tagulao Karen Araño , Bernardo Allan B. I. , Kei Loi Hoi , Calheiros Cristina Sousa Coutinho
Published: March 2022

Mangrove forests are one of the most ecologically valuable ecosystems in the world and provide a wide variety of ecosystem services to coastal communities, including cities. Macao, a highly urbanized coastal city located on the southern coast of China west of the Pearl River, is home to several species of mangroves with many associated flora and fauna. Mangrove forests in Macao are vulnerable to threats due to pressure from rapid and massive urban developments in the area, which led to mangrove loss in the past decades. To address this issue, the local authorities established special Ecological Zones for the management of the local mangroves. To reinforce local conservation efforts, educating the local population about the value of mangroves, especially school students, is of utmost importance. To evaluate the impact of environmental education activities on the environmental orientation, knowledge, and values of students toward mangrove conservation in Macao, a quasi-experimental study was undertaken. The effectiveness of a mangroves exhibition and field visit were evaluated using the New Environmental Paradigm (NEP) Scale—Macao version in a group of local school students who participated in the activities. Overall, the results provided consistently positive evaluations of the impact of the environmental education program. The strongest improvements were found in the students’ pro-environmental orientations, knowledge about mangroves, and value for environmental protection.
An Exploration of Narcan as a Harm Reduction Strategy and User’s Attitudes toward Law Enforcement Involvement in Overdose Cases
Authors: Durieux Jared , Curtis Andrew , Mirka Melissa , Jefferis Eric , Felix Chaz , Essel Baaba
Published: March 2022

The street homeless, those who spend their nights either in shelters or unofficial camps, whether in tents on a street or in society’s hidden spaces such as beneath an overpass, face multiple challenges beyond finding a safe place to sleep. Of further concern is how official actions can worsen these situations, through day-to-day activities or planned intervention strategies. In this paper we explore how a planned intervention may be negatively perceived—even as a form of “structural violence”—and may prevent Narcan (naloxone) use to stop an overdose related death in the Skid Row of Los Angeles. Data for this study consisted of a combination of Spatial Video Geonarratives (SVGs) and 325 incident reports from the Homeless Health Care Los Angeles Center for Harm Reduction (HHCLA-HRC) between November 2014 and December 2015. Chi-square and simple logistic regression models were used to examine the association between fear-of-arrest and other covariates of interest. Mapping results are presented with different sets of shapefiles created for (1) all Narcan uses, (2) all homeless, (3) all homeless with a worry about being arrested, (4) all Narcan uses where an ambulance attended, (5) and the same as 4 but also with police attendance. In the multivariable model, the estimated adjusted odds of fear-of-arrest is over three times higher among Narcan users ages 30–39 when compared to users under the age of 30. Analyzing the association of calling 9-1-1 on Narcan user demographics, socio-contextual characteristics, and overdose victim demographics, the crude estimated probability of calling 9-1-1 for Narcan users aged 50 and older is nearly three times higher when compared to Narcan users aged 19–29. Conclusion: Results suggest that the fear-of-arrest and calling 9-1-1 during an overdose is still a concern among Narcan users despite protective legislation and access to harm reduction resources.
Cross-Country Adaptation of a Psychological Flexibility Measure: The Comprehensive Assessment of Acceptance and Commitment Therapy Processes
Authors: Giovannetti Ambra , Pöttgen Jana , Anglada Elisenda , Menéndez Rebeca , Hoyer Jürgen , Giordano Andrea , Pakenham Kenneth , Galán Ingrid , Solari Alessandra
Published: March 2022

Purpose: The Comprehensive assessment of Acceptance and Commitment Therapy (ACT) processes (CompACT) is a 23-item self-report questionnaire assessing psychological flexibility, which is the overarching construct underpinning the ACT framework. We conducted a two-phase project to develop validated versions of the CompACT in three languages: phase 1—cross-cultural adaptation; and phase 2—psychometric validation of the questionnaire for use in Italy, Germany and Spain. This article focuses on the first phase. Methods: We translated and culturally adapted the CompACT in the three target languages, following the ISPOR TCA Task Force guidelines. The process was overseen by a translation panel (three translators, at least two multiple sclerosis (MS) researchers and a lay person), ACT experts and clinicians from the research team of each country and the original CompACT developers. We debriefed the new questionnaire versions via face-to-face interviews with a minimum of four adults from the general population (GP) and four adults with MS in each country. Results: The translation-adaptation process went smoothly in the three countries, with some items (7 in Italy, 4 in Germany, 6 in Spain) revised after feedback from ACT experts. Cognitive debriefing showed that the CompACT was deemed easy to understand and score in each target country by both GP and MS adults. Conclusions: The Italian, German and Spanish versions of the CompACT have semantic, conceptual and normative equivalence to the original scale and good content validity. Our findings are informative for researchers adapting the CompACT and other self-reported outcome measures into multiple languages and cultures.
Are Work Demand, Support and Control Associated with Work Ability and Disability during Back Pain Treatment? A Prospective Explorative Study
Authors: Unsgaard-Tøndel Monica , Nordstoga Anne Lovise
Published: March 2022

Background: Low back pain is a multifactorial disease with consequences for work ability and social participation. Improved integration of the work domain in health care management is needed. The aim of this study was to explore the relation between working conditions with outcome of low back pain treatment. Methods: Observational study of 41 patients attending physiotherapy for low back pain. Work demands, support and control were registered at baseline and work ability and disability also at baseline, with follow up after three and nine months. We used mixed-effects models to estimate the longitudinal associations between working conditions and outcome. Results: Higher work demands were related to reduced work ability (−1.1 points, 95% CI: −2.1 to −0.1) and slightly increased disability (5.6 points, 95% CI: 0.5 to 10.7). Lack of social support from colleagues was associated with reduced work ability (−2.7 points, 95% CI: −0.2 to 1.5) and disability (14.0 points, 95% CI: 4.9 to 23.1). Conclusions: This explorative study found associations between work demands and support, and work ability and disability outcome. Screening for psychosocial working conditions may influence the work ability and disability treatment outcome. The results need replication in larger samples and may indicate that patients seeking primary care management for low back pain should be screened for work demands, support and control.
Post-Discharge Clostridioides difficile Infection after Arthroplasties in Poland, Infection Prevention and Control as the Key Element of Prevention of C. difficile Infections
Authors: Jachowicz Estera , Pac Agnieszka , Różańska Anna , Gryglewska Barbara , Wojkowska-Mach Jadwiga
Published: March 2022

Clostridioides difficile is still one of the most common causes of hospital-acquired infectious diarrhea (CDI), and the incidence of CDI is one of the indicators that allows conclusions to be derived on the correctness of antibiotic administration. The objective of this observational study was the analysis of post-discharge CDI incidence in patients undergoing hip or knee arthroplasty, in order to specify optimum conditions for the surgical procedures and outpatient postoperative care. One-year observational study. Public Polish hospitals. Retrospective records for 83,525 surgery patients having undergone hip or knee arthroplasty were extracted from the Polish National Health Fund databases. CDI and/or antibiotic prescriptions in the 30 day post-surgery period were expressed per 1000 surgeries with antibiotic prescription on discharge or in ambulatory care, respectively. The CDI incidence rate was 34.4 per 10,000 patients, and 7.7 cases per 100,000 post-surgery patient-days. Patients who were prescribed at least one antibiotic were diagnosed with CDI more often than patients who had no antibiotic treatment (55.0/1000 patients vs. 1.8/1000 patients). In the multifactorial analysis, the following factors were significant: being at least 65 years of age, trauma as the cause of surgery, length of stay over 7 days, HAIs other than CDI and taking beta-lactams and/or quinolones but not macrolides in the post-discharge period. Postoperative antibiotic prescription in patients undergoing joint replacement surgery is the main risk factor for CDI. These observations indicate the necessity of improvement of infection control programs as the key factor for CDI prevention.
Incidence of Influenza Virus Infection among Wroclaw’s Healthcare Workers in Pre-COVID-19 2019–2020 Influenza Season Using Novel Flu SensDx Device
Authors: Jędrzejek Michał Jacek , Mastalerz-Migas Agnieszka , Janicka Paulina
Published: March 2022

Background: Healthcare workers (HCWs) are more exposed to influenza infection, and the influenza vaccination is recommended each year, to reduce the risk of influenza infection and prevent influenza transmission. This study is a cross-sectional study and the objectives were to determine the rate of influenza virus infection among HCWs in the 2019–2020 influenza season. Methods: Between January and March 2020, a survey was carried out in 2 hospitals and 15 primary health-care settings (PHCS) in Wroclaw (Poland). The novel point-of-care testing Flu SensDx device was used, which detects the M1 protein of the influenza virus using electrochemical impedance spectroscopy from biological material (throat/nasal swabs). Results: A total of 150 samples were collected. The majority of participating HCWs by profession were 83 physicians (55.3%) and half (51.3%) of the participating HCWs worked in PHCS. Influenza vaccination coverage was 61.3% in 2019–2020 and 46.0% in the 2018–2019 season for all participants. Of the participating HCWs, 44.0% were positive tested by the Flu SensDx device. There were no statistically significant differences among the positive tested HCWs, their influenza immunization history, and the presence of symptoms of influenza-like illness (p > 0.05). Conclusion: Although the results of the present study suggest that influenza vaccination does not reduce the frequency of influenza virus detection by Flu SensDx testing in the HCWs participants, larger studies are needed to estimate the incidence of influenza virus infection among HCWs to understand the underlying mechanism and fine-tune policies aimed at reducing nosocomial infections.
Comparison of Interactive Teaching in Online and Offline Platforms among Dental Undergraduates
Authors: Veeraiyan Deepak Nallaswamy , Varghese Sheeja S. , Rajasekar Arvina , Karobari Mohmed Isaqali , Thangavelu Lakshmi , Marya Anand , Messina Pietro , Scardina Giuseppe Alessandro
Published: March 2022

In recent years, the educational system has focused more on the holistic development of an individual. Modern technology has changed the educational environment to provide students with better academic opportunities. Along with the education system, teaching techniques and learning tools have also changed with digital evolution. This research was undertaken to assess the academic performance of interactive teaching methods in offline and online platforms in Periodontics among BDS undergraduates at a dental college in India. This prospective study was conducted among 49 students: Group I (n = 24, online class through Zoom) and Group II (n = 25, offline classes). The subject was divided into three modules and was covered in one week. The topics covered, teaching methods, lectures, and activities were similar for both groups. A formative assessment mark was obtained from written tests during the module, whereas the summative assessment mark was recorded from exams conducted towards the end of the module. In the results, a statistically significant difference was not observed in terms of formative assessment between Group I (77.88 ± 12.89) and Group II (77.80 ± 16.09) (p = 0.98). In addition, a statistically significant difference was not observed in terms of summative assessment between Group I (80.54 ± 8.39) and Group II (80.28 ± 11.57) (p = 0.93). Overall, this study suggests that interactive teaching methods in both offline and online platforms in Periodontics showed equivalent performance by the undergraduate dental students.
The Impact of the COVID-19 Pandemic on Hospital Services for Patients with Cardiac Diseases: A Scoping Review
Authors: de Lange Mats , Carvalho Ana Sofia , Brito Fernandes Óscar , Lingsma Hester , Klazinga Niek , Kringos Dionne
Published: March 2022

This study aims to assess the impact of the COVID-19 pandemic on hospital cardiac care, as assessed by performance indicators. Scoping review methodology: performance indicators were extracted to inform on changes in care during January–June 2020. Database searches yielded 6277 articles, of which 838 met the inclusion criteria. After full-text screening, 94 articles were included and 1637 indicators were retrieved. Most of the indicators that provided information on changes in the number of admissions (n = 118, 88%) signaled a decrease in admissions; 88% (n = 15) of the indicators showed patients’ delayed presentation and 40% (n = 54) showed patients in a worse clinical condition. A reduction in diagnostic and treatment procedures was signaled by 95% (n = 18) and 81% (n = 64) of the indicators, respectively. Length of stay decreased in 58% (n = 21) of the indicators, acute coronary syndromes treatment times increased in 61% (n = 65) of the indicators, and outpatient activity decreased in 94% (n = 17) of the indicators related to outpatient care. Telehealth utilization increased in 100% (n = 6). Outcomes worsened in 40% (n = 35) of the indicators, and mortality rates increased in 52% (n = 31). All phases of the pathway were affected. This information could support the planning of care during the ongoing pandemic and in future events.
Predictive Model and Mortality Risk Score during Admission for Ischaemic Stroke with Conservative Treatment
Authors: Lea-Pereira María Carmen , Amaya-Pascasio Laura , Martínez-Sánchez Patricia , Rodríguez Salvador María del Mar , Galván-Espinosa José , Téllez-Ramírez Luis , Reche-Lorite Fernando , Sánchez María-José , García-Torrecillas Juan Manuel
Published: March 2022

Background: Stroke is the second cause of mortality worldwide and the first in women. The aim of this study is to develop a predictive model to estimate the risk of mortality in the admission of patients who have not received reperfusion treatment. Methods: A retrospective cohort study was conducted of a clinical–administrative database, reflecting all cases of non-reperfused ischaemic stroke admitted to Spanish hospitals during the period 2008–2012. A predictive model based on logistic regression was developed on a training cohort and later validated by the “hold-out” method. Complementary machine learning techniques were also explored. Results: The resulting model had the following nine variables, all readily obtainable during initial care. Age (OR 1.069), female sex (OR 1.202), readmission (OR 2.008), hypertension (OR 0.726), diabetes (OR 1.105), atrial fibrillation (OR 1.537), dyslipidaemia (0.638), heart failure (OR 1.518) and neurological symptoms suggestive of posterior fossa involvement (OR 2.639). The predictability was moderate (AUC 0.742, 95% CI: 0.737–0.747), with good visual calibration; Pearson’s chi-square test revealed non-significant calibration. An easily consulted risk score was prepared. Conclusions: It is possible to create a predictive model of mortality for patients with ischaemic stroke from which important advances can be made towards optimising the quality and efficiency of care. The model results are available within a few minutes of admission and would provide a valuable complementary resource for the neurologist.
Concrete Paving Slabs for Comfort of Movement of Mobility-Impaired Pedestrians—A Survey
Authors: Wojnowska-Heciak Magdalena , Heciak Jakub , Kłak Adam
Published: March 2022

People with locomotion difficulties encounter many barriers in a pedestrian environment. Pavement quality has been shown to substantially affect pedestrian satisfaction in general, and its optimal design may contribute to reducing the stigma put on people with impairments. Our research involved a survey assessing perception and attitudes towards pavement quality and characteristics for pedestrian comfort. The classic correlation of the variables based on contingency tables was used to analyse data and to test whether respondents’ perceptions depended on certain profile features. The completion of the statistical inference was the use of advanced algorithms of the correspondence analysis method. The relationships between the variables were assessed optionally using the chi2 test. The study results revealed desirable parameters and features of paving surfaces. People who use manual wheelchairs and declare limb loss prefer medium- and large-format concrete pavers with smooth finishing and no bevelling. People with skeletal abnormalities or peripheral neuropathy prefer concrete slabs and surfaces made of small- or medium-sized panels arranged in a regular pattern, made of smooth non-slip concrete with narrow joints. Further laboratory tests are necessary to investigate whether increased water permeability of pavements constructed on eco-friendly base layers can curb damage to the pavement surface and provide long-term durability.
The Health-Seeking Behavior among Malaysian Adults in Urban and Rural Areas Who Reported Sickness: Findings from the National Health and Morbidity Survey (NHMS) 2019
Authors: Mohd Noh Sarah Nurain , Jawahir Suhana , Tan Yeung R’ong , Ab Rahim Iqbal , Tan Ee Hong
Published: March 2022

Understanding care-seeking behavior among urban and rural populations can help to support the planning and implementation of appropriate measures to improve health in the community. This study aims to determine the factors associated with the health-seeking behavior among Malaysian adults in urban and rural areas who reported sickness. This study used data of Malaysian adults aged 18 years and over from the National Health and Morbidity Survey 2019; a cross-sectional, national household survey that targeted all non-institutionalized residents in Malaysia. Respondent’s characteristics and health-seeking behavior were described using complex sample descriptive statistics. Multivariable logistic regression analysis was conducted to examine the association between potential factors (sociodemographic characteristics, enabling, and health need) and health-seeking behaviors (seeking treatment from healthcare practitioners and self-medication). A total of 10,484 respondents, estimated to represent 18.9 million Malaysian adults aged 18 years and over, were included in the analysis. Prevalence of seeking treatment from healthcare practitioners and self-medication among Malaysian adults with self-reported sickness were 57.3% and 23.3%, respectively. Self-reported sickness among both the urban and rural populations who rated their health as poor to very poor was more likely to seek treatment than those who rated good to excellent. However, among the urban population, those who rated their health as poor to very poor were less likely to self-medicate. Among the urban population, government employees were more likely to seek treatment, and being without formal education significantly increased the likelihood to self-medicate. Among the rural population, those with at least one long-term condition were more likely to seek treatment than those with none. Understanding the factors which influence health-seeking behavior among the urban and rural population could close the gaps in healthcare utilization among the population in Malaysia.
Geography Education in a Collaborative Virtual Environment: A Qualitative Study on Geography Teachers
Authors: Jochecová Kateřina , Černý Michal , Stachoň Zdeněk , Švedová Hana , Káčová Natálie , Chmelík Jiří , Brůža Vojtěch , Kvarda Ondřej , Ugwitz Pavel , Šašinková Alžběta , Fořtová Nicola Catherine , Šašinka Čeněk
Published: March 2022

The presented study aspires to utilize the gradually validated immense potential of collaborative immersive virtual environments (CIVEs) in higher education when designing and conducting geography lessons. These particular lessons focused on hypsography. A Research through Design approach and relevant qualitative methodology were used as we engaged two groups of domain experts (experienced geography teachers) to validate both the learning scenarios and the virtual environment we used. The lessons were administered via eDIVE—a novel platform for collaborative learning and teaching in virtual reality of our own design. The teachers underwent a hypsography virtual lesson and were randomly divided into two groups to be compared, which differed in the level of structure given to the lesson (one group received detailed instructions on what task they were to solve, while the other was given a free hand in exploring the environment and activities it afforded). The teachers’ experiences were then summarized in a post-lesson reflection and a subsequent focus group following the tasks. The participants’ expertise allowed insights to be gained into their first-hand experience as students, as well as their expert view of the lesson from an educational point of view. Virtual reality’s implementation into teaching practice was the key topic of the discussion.
An Application of Improved MODIS-Based Potential Evapotranspiration Estimates in a Humid Tropic Brantas Watershed—Implications for Agricultural Water Management
Authors: Astuti Ike Sari , Wiwoho Bagus Setiabudi , Purwanto Purwanto , Wagistina Satti , Deffinika Ifan , Sucahyo Hetty Rahmawati , Herlambang Gilang Aulia , Alfarizi Imam Abdul Gani
Published: March 2022

The reliance on native MODIS-16 PET potential evapotranspiration (PET) in scarce-data-driven areas is growing in support among ecohydrological studies, yet information about its performance is limited or unknown as validation studies are mostly concentrated in developed countries. This study aimed to assess its performance at the monthly level using four ground measurements in a tropical watershed system with complex topography, applying a machine learning artificial neural network (ANN) to improve the estimates, and using the ANN-adjusted MODIS-16 PET to characterize the spatio-temporal patterns of PET in the Brantas watershed, as well as to understand the monthly patterns of water deficiency in areas under eight different vegetation covers. The results showed that the native MODIS-16 PET experienced overestimation with an RMSE of 37–66 mm/month and NRSME of up to 33%. The performance decreased in drier periods. The ANN-based adjustment using only one variable showed improved estimates with a reduction of RSME to only 14 mm and lower than 10% NRMSE. Sari-temporal patterns of PET in the Brantas watershed showed that the PET characteristics were not uniform. The southern part of the Brantas watershed has areas with relatively lower PET that are, thus, more prone to water deficiency. Complex topography and climate gradients within the watershed apparently became the multi-controllers of PET variations. The difference in vegetation cover also influenced the magnitudes of water deficiency.
Early Effects of Extracellular Vesicles Secreted by Adipose Tissue Mesenchymal Cells in Renal Ischemia Followed by Reperfusion: Mechanisms Rely on a Decrease in Mitochondrial Anion Superoxide Production
Authors: Lopes Jarlene A. , Collino Federica , Rodrigues-Ferreira Clara , Sampaio Luzia da Silva , Costa-Sarmento Glória , Wendt Camila H. C. , Almeida Fernando P. , Miranda Kildare R. , Kasai-Brunswick Tais H. , Lindoso Rafael S. , Vieyra Adalberto
Published: March 2022

Acute kidney injury (AKI) caused by ischemia followed by reperfusion (I/R) is characterized by intense anion superoxide (O2•−) production and oxidative damage. We investigated whether extracellular vesicles secreted by adipose tissue mesenchymal cells (EVs) administered during reperfusion can suppress the exacerbated mitochondrial O2•− formation after I/R. We used Wistar rats subjected to bilateral renal arterial clamping (30 min) followed by 24 h of reperfusion. The animals received EVs (I/R + EVs group) or saline (I/R group) in the kidney subcapsular space. The third group consisted of false-operated rats (SHAM). Mitochondria were isolated from proximal tubule cells and used immediately. Amplex Red™ was used to measure mitochondrial O2•− formation and MitoTracker™ Orange to evaluate inner mitochondrial membrane potential (Δψ). In vitro studies were carried out on human renal proximal tubular cells (HK-2) co-cultured or not with EVs under hypoxic conditions. Administration of EVs restored O2•− formation to SHAM levels in all mitochondrial functional conditions. The gene expression of catalase and superoxide dismutase-1 remained unmodified; transcription of heme oxygenase-1 (HO-1) was upregulated. The co-cultures of HK-2 cells with EVs revealed an intense decrease in apoptosis. We conclude that the mechanisms by which EVs favor long-term recovery of renal structures and functions after I/R rely on a decrease of mitochondrial O2•− formation with the aid of the upregulated antioxidant HO-1/Nuclear factor erythroid 2-related factor 2 system, thus opening new vistas for the treatment of AKI.
Propagation and Dissemination Strategies of Transmissible Spongiform Encephalopathy Agents in Mammalian Cells
Authors: Heumüller Stefanie-Elisabeth , Hornberger Annika C. , Hebestreit Alina S. , Hossinger André , Vorberg Ina M.
Published: March 2022

Transmissible spongiform encephalopathies or prion disorders are fatal infectious diseases that cause characteristic spongiform degeneration in the central nervous system. The causative agent, the so-called prion, is an unconventional infectious agent that propagates by converting the host-encoded cellular prion protein PrP into ordered protein aggregates with infectious properties. Prions are devoid of coding nucleic acid and thus rely on the host cell machinery for propagation. While it is now established that, in addition to PrP, other cellular factors or processes determine the susceptibility of cell lines to prion infection, exact factors and cellular processes remain broadly obscure. Still, cellular models have uncovered important aspects of prion propagation and revealed intercellular dissemination strategies shared with other intracellular pathogens. Here, we summarize what we learned about the processes of prion invasion, intracellular replication and subsequent dissemination from ex vivo cell models.
Antibody Responses to Transglutaminase 3 in Dermatitis Herpetiformis: Lessons from Celiac Disease
Authors: Kaunisto Helka , Salmi Teea , Lindfors Katri , Kemppainen Esko
Published: March 2022

Dermatitis herpetiformis (DH) is the skin manifestation of celiac disease, presenting with a blistering rash typically on the knees, elbows, buttocks and scalp. In both DH and celiac disease, exposure to dietary gluten triggers a cascade of events resulting in the production of autoantibodies against the transglutaminase (TG) enzyme, mainly TG2 but often also TG3. The latter is considered to be the primary autoantigen in DH. The dynamics of the development of the TG2-targeted autoimmune response have been studied in depth in celiac disease, but the immunological process underlying DH pathophysiology is incompletely understood. Part of this process is the occurrence of granular deposits of IgA and TG3 in the perilesional skin. While this serves as the primary diagnostic finding in DH, the role of these immunocomplexes in the pathogenesis is unknown. Intriguingly, even though gluten-intolerance likely develops initially in a similar manner in both DH and celiac disease, after the onset of the disease, its manifestations differ widely.
A Rule-Based Heuristic Methodology for Su-Field Analysis in Industrial Engineering Design
Authors: Yan Wei , Zanni-Merk Cecilia , Cavallucci Denis , Cao Qiushi , Zhang Liang , Ji Zengyan
Published: March 2022

Industrial engineering design is a crucial issue in manufacturing. To meet the competitive global market, manufacturers are continuously seeking solutions to design industrial products and systems inventively. Su-Field analysis, which is one of the TRIZ analysis tools for inventive design problems, has been used to effectively improve the performance of industrial systems. However, the inventive standards used for engineering design are summarized and classified according to a large number of patents in different fields. They are built on a highly abstract basis and are independent of specific application fields, making their use require much more technical knowledge than other TRIZ tools. To facilitate the use of invention standards, in particular to capture the uncertainty or imprecision described in the standards, this paper proposes a rule-based heuristic approach. First, Su-Field analysis ontology and fuzzy analysis ontology are constructed to represent precise and fuzzy knowledge in the process of solving inventive problems respectively. Then, SWRL (Semantic Web Rule Language) reasoning and fuzzy reasoning are executed to generate heuristic conceptual solutions. Finally, we develop a software prototype and elaborate the resolution of “Auguste Piccard’s Stratostat ” in the prototype.
A Multicenter, Randomized, Double-Blinded, Parallel-Group, Placebo-Controlled Phase I/IIa Study to Evaluate the Efficacy and Safety of a Single Intra-Articular Injection of YYD302 in Patients with Knee Osteoarthritis
Authors: In Yong , Ha Chul-Won
Published: March 2022

This study was a phase I/IIa, multicenter, randomized, double-blinded, parallel, placebo-controlled clinical trial that aimed to assess the efficacy and safety of a single intra-articular injection of YYD302, a novel high-molecular-weight hyaluronic acid with divinyl sulfone cross-linking. Thirty adults with knee osteoarthritis were randomized to receive a single 2 mL intra-articular injection of YYD302 (test group 1), 3 mL of YYD302 (test group 2), or 3 mL of the placebo (placebo group). We compared the changes from the baseline in the weight-bearing pain of 100 mm using the Visual Analog Scale (VAS), the Knee Injury and Osteoarthritis Outcome Score (KOOS), the Rheumatology-Osteoarthritis Research Society International (OMERACT-OASRSI) responder rates, and the use of rescue analgesics to assess the safety of this novel drug. A total of 26 subjects (10 in test group 1, 10 in test group 2, and 6 in the placebo group) were included in the full analysis set. At 12 weeks, only test groups 1 and 2 showed significant changes in the weight-bearing pain VAS scores (p = 0.0015 and p = 0.0085), symptoms, and average daily KOOS values compared to the baseline (p < 0.001, p = 0.0124, and p = 0.0018, p = 0.0426, respectively). While the rate and frequency of consuming the rescue drug continued to increase in the placebo group until 12 weeks, there was no change in the test groups. Our findings showed that YYD302, especially 2 mL of YYD302, reduced pain and improved knee joint function compared to the placebo.
Study on Applicability of Energy-Saving Devices to Hydrogen Fuel Cell-Powered Ships
Authors: Stark Callum , Xu Yunxin , Zhang Ming , Yuan Zhiming , Tao Longbin , Shi Weichao
Published: March 2022

The decarbonisation of waterborne transport is arguably the biggest challenge faced by the maritime industry presently. By 2050, the International Maritime Organization (IMO) aims to reduce greenhouse gas emissions from the shipping industry by 50% compared to 2008, with a vision to phase out fossil fuels by the end of the century as a matter of urgency. To meet such targets, action must be taken immediately to address the barriers to adopt the various clean shipping options currently at different technological maturity levels. Green hydrogen as an alternative fuel presents an attractive solution to meet future targets from international bodies and is seen as a viable contributor within a future clean shipping vision. The cost of hydrogen fuel—in the short-term at least—is higher compared to conventional fuel; therefore, energy-saving devices (ESDs) for ships are more important than ever, as implementation of rules and regulations restrict the use of fossil fuels while promoting zero-emission technology. However, existing and emerging ESDs in standalone/combination for traditional fossil fuel driven vessels have not been researched to assess their compatibility for hydrogen-powered ships, which present new challenges and considerations within their design and operation. Therefore, this review aims to bridge that gap by firstly identifying the new challenges that a hydrogen-powered propulsion system brings forth and then reviewing the quantitative energy saving capability and qualitive additional benefits of individual existing and emerging ESDs in standalone and combination, with recommendations for the most applicable ESD combinations with hydrogen-powered waterborne transport presented to maximise energy saving and minimise the negative impact on the propulsion system components. In summary, the most compatible combination ESDs for hydrogen will depend largely on factors such as vessel types, routes, propulsion, operation, etc. However, the mitigation of load fluctuations commonly encountered during a vessels operation was viewed to be a primary area of interest as it can have a negative impact on hydrogen propulsion system components such as the fuel cell; therefore, the ESD combination that can maximise energy savings as well as minimise the fluctuating loads experienced would be viewed as the most compatible with hydrogen-powered waterborne transport.
Development of a webGIS Application to Assess Conflicting Activities in the Framework of Marine Spatial Planning
Authors: Patera Anastasia , Pataki Zoi , Kitsiou Dimitra
Published: March 2022

Marine spatial planning (MSP) has been established as the appropriate policy framework to study and resolve conflicts that arise among various activities. A pre-requisite for the successful implementation of MSP is the availability of efficient tools to support decision-makers and enhance stakeholders’ engagement. In this paper, a webGIS application is proposed that is able to assess the intensity of conflicts among marine activities; the area of the Cyclades in the Aegean Sea was used as a case study. The webGIS application allows the visualization of existing activities, the delineation of conflicting activities, the detection of areas where multiple conflicts co-exist, and the delineation of areas of conflicts based on specific criteria. The webGIS application is available via a user-friendly interface as well as allowing interaction with users by providing them the opportunity to comment on the results and/or exchange ideas with other users of various groups; therefore, the participatory process, a creative stage in MSP, is further supported. The usefulness of such tools in coastal and marine planning and the decision-making process are further discussed.
Seventeen Ustilaginaceae High-Quality Genome Sequences Allow Phylogenomic Analysis and Provide Insights into Secondary Metabolite Synthesis
Authors: Ullmann Lena , Wibberg Daniel , Busche Tobias , Rückert Christian , Müsgens Andreas , Kalinowski Jörn , Blank Lars M.
Published: March 2022

The family of Ustilaginaceae belongs to the order of Basidiomycetes. Despite their plant pathogenicity causing, e.g., corn smut disease, they are also known as natural producers of value-added chemicals such as extracellular glycolipids, organic acids, and polyols. Here, we present 17 high-quality draft genome sequences (N50 > 1 Mb) combining third-generation nanopore and second-generation Illumina sequencing. The data were analyzed with taxonomical genome-based bioinformatics methods such as Percentage of Conserved Proteins (POCP), Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) analyses indicating that a reclassification of the Ustilaginaceae family might be required. Further, conserved core genes were determined to calculate a phylogenomic core genome tree of the Ustilaginaceae that also supported the results of the other phylogenomic analysis. In addition, to genomic comparisons, secondary metabolite clusters (e.g., itaconic acid, mannosylerythritol lipids, and ustilagic acid) of biotechnological interest were analyzed, whereas the sheer number of clusters did not differ much between species.