bluuebunny/crossref_metadata_2025 huggingface dataset:
Emerging Epigenetic Targets and Their Molecular Impact on Vascular Remodeling in Pulmonary Hypertension
Authors: Ranasinghe A. Dushani C. U. , Tennakoon T. M. Parinda B. , Schwarz Margaret A.
Published: January 2024

Pulmonary Hypertension (PH) is a terminal disease characterized by severe pulmonary vascular remodeling. Unfortunately, targeted therapy to prevent disease progression is limited. Here, the vascular cell populations that contribute to the molecular and morphological changes of PH in conjunction with current animal models for studying vascular remodeling in PH will be examined. The status quo of epigenetic targeting for treating vascular remodeling in different PH subtypes will be dissected, while parallel epigenetic threads between pulmonary hypertension and pathogenic cancer provide insight into future therapeutic PH opportunities.
Update on Targeted Therapy and Immunotherapy for Metastatic Colorectal Cancer
Authors: Underwood Patrick W. , Ruff Samantha M. , Pawlik Timothy M.
Published: January 2024

Metastatic colorectal cancer remains a deadly malignancy and is the third leading cause of cancer-related death. The mainstay of treatment for metastatic colorectal cancer is chemotherapy, but unfortunately, even with recent progress, overall survival is still poor. Colorectal cancer is a heterogeneous disease, and the underlying genetic differences among tumors can define the behavior and prognosis of the disease. Given the limitations of cytotoxic chemotherapy, research has focused on developing targeted therapy based on molecular subtyping. Since the early 2000s, multiple targeted therapies have demonstrated efficacy in treating metastatic colorectal cancer and have received FDA approval. The epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), and DNA mismatch repair pathways have demonstrated promising results for targeted therapies. As new gene mutations and proteins involved in the oncogenesis of metastatic colorectal cancer are identified, new targets will continue to emerge. We herein provide a summary of the updated literature regarding targeted therapies for patients with mCRC.
Gestational Intermittent Hypoxia Enhances Mammary Stem Cells and Alters Tumor Phenotype in Adult Female Offspring
Authors: Joshi Jaitri , Xiong Yue , Kuhn Molly , Radcliff Abigail B. , Baker Tracy L. , Watters Jyoti J. , Arendt Lisa M.
Published: January 2024

An adverse perinatal environment can increase long-term cancer risk, although the precise nature of associated perinatal triggers remain unknown. Sleep apnea is a common condition during pregnancy, characterized by recurrent cessations in breathing during sleep, and the potential consequences of sleep apnea during pregnancy as it relates to breast cancer risk in offspring have not been explored. To model sleep apnea, Sprague-Dawley dams were exposed during gestation to nightly intermittent hypoxia (GIH) or normoxia (GNx), and the mammary glands of female offspring were examined. GIH offspring demonstrated increased epithelial stem and progenitor cell populations, which are associated with diminished transforming growth factor beta (TGFβ) activity. Elevations in adipose tissue stem cells in the mammary gland were also identified in GIH offspring. In aging females, mammary tumors formed in GIH offspring. These tumors displayed a dramatic increase in stroma compared to tumors from GNx offspring, as well as distinct patterns of expression of stem cell-related pathways. Together, these results suggest that exposure to sleep apnea during pregnancy leads to lasting changes in the mammary glands of female offspring. Increased stem and progenitor cell populations as a result of GIH exposure could enhance long-term breast cancer risk, as well as alter the clinical behavior of resulting breast tumors.
Characterization of pSer129-αSyn Pathology and Neurofilament Light-Chain Release across In Vivo, Ex Vivo, and In Vitro Models of Pre-Formed-Fibril-Induced αSyn Aggregation
Authors: Hansen Maja L. , Ambjørn Malene , Harndahl Mikkel N. , Benned-Jensen Tau , Fog Karina , Bjerregaard-Andersen Kaare , Sotty Florence
Published: January 2024

Protein aggregation is a predominant feature of many neurodegenerative diseases, including synucleinopathies, which are characterized by cellular inclusions containing α-Synuclein (αSyn) phosphorylated at serine 129 (pSer129). In the present study, we characterized the development of αSyn pre-formed fibril (PFF)-induced pSer129-αSyn pathology in F28tg mice overexpressing human wild-type αSyn, as well as in ex vivo organotypic cultures and in vitro primary cultures from the same mouse model. Concurrently, we collected cerebrospinal fluid (CSF) from mice and conditioned media from ex vivo and in vitro cultures and quantified the levels of neurofilament light chain (NFL), a biomarker of neurodegeneration. We found that the intra-striatal injection of PFFs induces the progressive spread of pSer129-αSyn pathology and microglial activation in vivo, as well as modest increases in NFL levels in the CSF. Similarly, PFF-induced αSyn pathology occurs progressively in ex vivo organotypic slice cultures and is accompanied by significant increases in NFL release into the media. Using in vitro primary hippocampal cultures, we further confirmed that pSer129-αSyn pathology and NFL release occur in a manner that correlates with the fibril dose and the level of the αSyn protein. Overall, we demonstrate that αSyn pathology is associated with NFL release across preclinical models of seeded αSyn aggregation and that the pharmacological inhibition of αSyn aggregation in vitro also significantly reduces NFL release.
Mechanisms of Action and Preservation Effects of Packaging Systems for Mushrooms: Novel Approaches to Preserve Irish Edible Mushrooms
Authors: Shonte Tigist Tadesse , Mulla Mehraj Fatema , Foley Lorraine , Pathania Shivani
Published: January 2024

Mushrooms support the Irish economy, with a farm gate value of roughly EUR 130 million in 2022 and with 80%–85% of Irish production exported to the United Kingdom. In order to apply cutting edge technologies and offer creative solutions to increase the shelf life of mushrooms, it is essential to understand the mechanisms of action and preservation effects of the current trends in edible mushroom packaging systems. This review summarises the mechanisms of action for nanopackaging, biodegradable packaging, edible coatings, modified atmosphere packaging (MAP), and active packaging in terms of their enzyme activity, antimicrobial activity, antioxidant activity, and rate of respiration along with the changes in texture, colour, nutritional value, and shelf life of mushrooms reflected in the preservation effects of these packaging systems. SWOT analysis highlights the strengths, weaknesses, and threats of these packaging systems and provides potential opportunities for trialing innovative packaging materials for fresh edible mushrooms in Ireland.
Error Pattern Discovery in Spellchecking Using Multi-Class Confusion Matrix Analysis for the Croatian Language
Authors: Gledec Gordan , Sokele Mladen , Horvat Marko , Mikuc Miljenko
Published: January 2024

This paper introduces a novel approach to the creation and application of confusion matrices for error pattern discovery in spellchecking for the Croatian language. The experimental dataset has been derived from a corpus of mistyped words and user corrections collected since 2008 using the Croatian spellchecker available at ispravi.me. The important role of confusion matrices in enhancing the precision of spellcheckers, particularly within the diverse linguistic context of the Croatian language, is investigated. Common causes of spelling errors, emphasizing the challenges posed by diacritic usage, have been identified and analyzed. This research contributes to the advancement of spellchecking technologies and provides a more comprehensive understanding of linguistic details, particularly in languages with diacritic-rich orthographies, like Croatian. The presented user-data-driven approach demonstrates the potential for custom spellchecking solutions, especially considering the ever-changing dynamics of language use in digital communication.
Surface Modification of Bi2Te3 Nanoplates Deposited with Tin, Palladium, and Tin/Palladium Using Electroless Deposition
Authors: Kohashi Kaito , Okano Yutaro , Tanisawa Daiki , Kaneko Keisuke , Miyake Shugo , Takashiri Masayuki
Published: January 2024

Surface-modified nanoplate-shaped thermoelectric materials can achieve good thermoelectric performance. Herein, single-crystalline Bi2Te3 nanoplates with regular hexagonal shapes were prepared via solvothermal techniques. Surface modification was performed to deposit different metals onto the nanoplates using electroless deposition. Nanoparticle-shaped tin (Sn) and layer-shaped palladium (Pd) formed on the Bi2Te3 nanoplates via electroless deposition. For the sequential deposition of Sn and Pd, the surface morphology was mostly the same as that of the Sn-Bi2Te3 nanoplates. To assess the thermoelectric properties of the nanoplates as closely as possible, they were compressed into thin bulk shapes at 300 K. The Sn-Bi2Te3 and Sn/Pd-Bi2Te3 nanoplates exhibited the lowest lattice thermal conductivity of 1.1 W/(m·K), indicating that nanoparticle-shaped Sn facilitated the scattering of phonons. By contrast, the Pd-Bi2Te3 nanoplates exhibited the highest electrical conductivity. Thus, the highest power factor (15 μW/(m∙K2)) and dimensionless ZT (32 × 10−3) were obtained for the Pd-Bi2Te3 nanoplates. These thermoelectric properties were not as high as those of the sintered Bi2Te3 samples; however, this study revealed the effect of different metal depositions on Bi2Te3 nanoplates for improving thermoelectric performance. These findings offer venues for improving thermoelectric performance by sintering nanoplates deposited with appropriate metals.
Synthesis, Crystal Structure and Supramolecular Features of Novel 2,4-Diaminopyrimidine Salts
Authors: Bojarska Joanna , Łyczko Krzysztof , Mieczkowski Adam
Published: January 2024

The crystal structures and the supramolecular architectures of a series of novel salts originating from 2,4-diaminopyrimidine and four different chain dicarboxylic acids are reported. For this purpose, 2,4-diaminopyrimidin-1-ium 2,2′-thio(acetic)acetate (1), 2,4-diaminopyrimidin-1-ium monoglutarate (2), 2,4-diaminopyrimidin-1-ium 3,3′-dithio(propionic)propionate (3) and 2,4-diaminopyrimidin-1-ium suberate (4) were synthesized in good to high yields from 2,4-diaminopyrimidine and appropriate dicarboxylic acids (2,2′-thiodiacetic acid, glutaric acid, 3,3′-dithiodipropionic acid and suberic acid, respectively). Each of the compounds were formed as a monohydrate and compound 4 additionally co-crystallized with the suberic acid molecule. Despite the similar structures of compounds 1 and 2 as well as 3 and 4, subtle but important differences are observed in their crystal packing and H-bonding patterns, especially between 3 and 4. Supramolecular self-assemblies can be distinguished through different interactions considering anions, leading to diverse H-bonding motifs, which also include sulphur atoms in 1 and 3, at the upper level of supramolecular architecture. Notably, the basic motif is always the same—2,4-diaminopyrimidine-based homosynthon R22(8) via N-H∙∙∙N interactions. The impact of diverse types of intermolecular interactions was evaluated by Hirshfeld analysis, while the propensity of atom pairs of elements to build interactions was calculated using enrichment ratios. Although compounds 1 and 3 contain S-atoms, the percentage of S-derived interactions is rather low. In 1, the contribution of S∙∙∙H/H∙∙∙S, S∙∙∙C/C∙∙∙S, S∙∙∙N/N∙∙∙S intermolecular contacts is 5.7%. In 2, the contribution of S∙∙∙H/H∙∙∙S accounts for only 0.6%.
Dinuclear Molybdenum(VI) Complexes Based on Flexible Succinyl and Adipoyl Dihydrazones
Authors: Topić Edi , Damjanović Vladimir , Pičuljan Katarina , Rubčić Mirta
Published: January 2024

A series of molybdenum(VI) complexes with aryl-functionalized alkyl dihydrazones was prepared by the reaction of [MoO2(acac)2] and the appropriate dihydrazone in methanol. Their solid-state structures were elucidated via single-crystal X-ray diffraction (SC-XRD) and Fourier-transform infra-red (FTIR) spectroscopy, while the thermal stability of compounds was inspected by combined thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) experiments. The behaviour of complexes in DMSO-d6 solution was explored by nuclear magnetic resonance (NMR). The relevant data show that all complexes are dinuclear, with dihydrazones acting as ditopic hexadentate ligands. The in vitro cytotoxic activity of the prepared molybdenum(VI) complexes was evaluated on THP-1 and HepG2 cell lines, while their antibacterial activity was tested against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Moraxella catarrhalis bacteria. The majority of compounds proved to be non-cytotoxic, while some exhibited superior antibacterial activity in comparison to dihydrazone ligands.
Investigating the Influence of Three Different Atmospheric Conditions during the Synthesis Process of NMC811 Cathode Material
Authors: Tiozzo Arianna , Ghaseminezhad Keyhan , Mazzucco Asya , Giuliano Mattia , Rocca Riccardo , Dotoli Matteo , Nicol Giovanna , Nervi Carlo , Baricco Marcello , Sgroi Mauro Francesco
Published: January 2024

Lithium-ion batteries (LIBs) are fundamental for the energetic transition necessary to contrast climate change. The characteristics of cathode active materials (CAMs) strongly influence the cell performance, so improved CAMs need to be developed. Currently, Li(Ni0.8Mn0.1Co0.1)O2 (NMC811) is state-of-the-art among the cathodic active materials. The aim of this work is the optimization of the procedure to produce NMC811: two different syntheses were investigated, the co-precipitation and the self-combustion methods. For a better understanding of the synthesis conditions, three different types of atmospheres were tested during the calcination phase: air (partially oxidizing), oxygen (totally oxidizing), and nitrogen (non-oxidizing). The synthesized oxides were characterized by X-ray Powder Diffraction (XRPD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), Inductively Coupled Plasma (ICP), and Particle Size Distribution (PSD). The most promising materials were tested in a half-cell set up to verify the electrochemical performances. The procedure followed during this study is depicted in the graphical abstract. The oxidizing atmospheric conditions turned out to be the most appropriate to produce NMC811 with good electrochemical properties.
Analysis of Epicardial Adipose Tissue Texture in Relation to Coronary Artery Calcification in PCCT: The EAT Signature!
Authors: Mundt Peter , Hertel Alexander , Tharmaseelan Hishan , Nörenberg Dominik , Papavassiliu Theano , Schoenberg Stefan O. , Froelich Matthias F. , Ayx Isabelle
Published: January 2024

(1) Background: Epicardial adipose tissue influences cardiac biology in physiological and pathological terms. As it is suspected to be linked to coronary artery calcification, identifying improved methods of diagnostics for these patients is important. The use of radiomics and the new Photon-Counting computed tomography (PCCT) may offer a feasible step toward improved diagnostics in these patients. (2) Methods: In this retrospective single-centre study epicardial adipose tissue was segmented manually on axial unenhanced images. Patients were divided into three groups, depending on the severity of coronary artery calcification. Features were extracted using pyradiomics. Mean and standard deviation were calculated with the Pearson correlation coefficient for feature correlation. Random Forest classification was applied for feature selection and ANOVA was performed for group comparison. (3) Results: A total of 53 patients (32 male, 21 female, mean age 57, range from 21 to 80 years) were enrolled in this study and scanned on the novel PCCT. “Original_glrlm_LongRunEmphasis”, “original_glrlm_RunVariance”, “original_glszm_HighGrayLevelZoneEmphasis”, and “original_glszm_SizeZoneNonUniformity” were found to show significant differences between patients with coronary artery calcification (Agatston score 1–99/≥100) and those without. (4) Conclusions: Four texture features of epicardial adipose tissue are associated with coronary artery calcification and may reflect inflammatory reactions of epicardial adipose tissue, offering a potential imaging biomarker for atherosclerosis detection.
Comparative Cyto-Histological Genetic Profile in a Series of Differentiated Thyroid Carcinomas
Authors: Matos Maria de Lurdes , Pinto Mafalda , Alves Marta , Canberk Sule , Gonçalves Ana , Bugalho Maria João , Papoila Ana Luísa , Soares Paula
Published: January 2024

Introduction: Molecular tests can contribute to improve the preoperative diagnosis of thyroid nodules. Tests available are expensive and not adapted to different populations. Aim: This study aimed to compare the cyto-histological genetic profile and to evaluate the reliability of molecular tests using ultrasound-guided fine needle aspiration cytology (US-FNAC) in accurately diagnosing differentiated thyroid carcinomas (DTCs) and predicting biologic behavior of papillary thyroid carcinomas (PTCs). Materials and Methods: The series included 259 patients with paired cyto-histological samples totaling 518 samples. The genetic alterations were analyzed via PCR/Sanger sequencing. The association with clinicopathologic features was evaluated in PTCs. Results/Discussion: From the 259 patients included, histologies were 50 (19.3%) benign controls and 209 (80.7%) DTC cases, from which 182 were PTCs; cytologies were 5.8% non-diagnostic, 18.2% benign, 39% indeterminate, and 37.1% malignant. In histology, indeterminate nodules (n = 101) were 22.8% benign and 77.2% malignant. Mutation frequencies in cytology and histology specimens were, respectively, TERTp: 3.7% vs. 7.9%; BRAF: 19.5% vs. 25.1%; and RAS: 11% vs. 17.5%. The overall cyto-histological agreement of the genetic mutations was 94.9%, with Cohen’s k = 0.67, and in indeterminate nodules agreement was 95.7%, k = 0.64. The identified mutations exhibited a discriminative ability in diagnosing DTC with a specificity of 100% for TERTp and BRAF, and of 94% for RAS, albeit with low sensitivity. TERTp and BRAF mutations were associated with aggressive clinicopathological features and tumor progression in PTCs (p < 0.001). The obtained good cyto-histological agreement suggests that molecular analysis via US-FNAC may anticipate the genetic profile and the behavior of thyroid tumors, confirming malignancy and contributing to referring patients to surgery.
Screening for Asymptomatic Osteonecrosis of the Hip in Systemic Lupus Erythematous: A Systematic Review and Meta-Analysis of MRI-Based Prevalence
Authors: Ibad Hamza A. , Ghotbi Elena , Kasaeian Arta , Levin Adam S. , Jones Lynne C. , Anzai Yoshimi , Soltanolkotabi Maryam , Kapoor Neena , Johnson Pamela T. , Demehri Shadpour
Published: January 2024

Objective. This paper aims to estimate asymptomatic hip osteonecrosis prevalence in SLE patients using MRI examination and to determine the prevalence among higher risk subpopulations. Materials and Methods. PubMed, Embase, Cochrane, and SCOPUS were searched from inception to May 9th, 2023. Studies on patients who were clinically diagnosed with systemic lupus erythematosus without reported symptoms attributable to hip osteonecrosis were included. Two independent reviewers extracted data and assessed the risk of bias. Data collected from each study include the study year, the number of hips screened, the number of hips with osteonecrosis, demographics, laboratory data, medications, follow-up time, radiological protocols, and MRI-based osteonecrosis detection and grading criteria. Results. Eleven eligible studies including 503 participants (15–35 years old; 74–100% female) with SLE were identified. Significant risk of bias was determined in one study. The overall prevalence of osteonecrosis of the hip was found to be 14% (184/1006 hip joints, 95% confidence interval: 7–22%, number needed to scan: 7.1). SLE patients who received corticosteroid treatment had a higher prevalence of asymptomatic hip osteonecrosis (18%) compared to non-corticosteroid users (0%, p-value < 0.01). Additionally, meta-regression results revealed that daily corticosteroid dose was associated with increased prevalence of asymptomatic osteonecrosis (0.5%/milligram, p-value < 0.01). Conclusions. The high prevalence of asymptomatic hip osteonecrosis in SLE patients raises concerns about the timeliness of interventions. The limitations of this study include a relatively low number of identified studies; and one study lacked full-text availability.
The Relationship between Types of Os Trigonum and Findings of Conventional Ankle Magnetic Resonance Imaging: A Study Based on Three-Dimensional Magnetic Resonance Imaging
Authors: Lee Kyu-Hong , Lee Ro-Woon , Kim Yeo-Ju
Published: January 2024

This study aimed to investigate the dimensions and types of the os trigonum and evaluate their relationship with various pathologic conditions on the posterior ankle using ankle MRI images. A total of 124 non-contrast-enhanced ankle and foot MR images of 123 consecutive patients were included in this retrospective study. The images were presented randomly, and they contained no patient information. The MR images were retrospectively and independently reviewed by two reviewers with a fellowship-trained musculoskeletal radiologist. The images were classified as type I and II based on the ossicle’s medial border overlying the talus’s posterior process and the groove for the flexor hallucis longus tendon (FHL). The study revealed that patients with type II os trigonum had a longer transverse diameter of the ossicle than type I, and there were statistically significant differences. Detachment status tended to be less in type I than in type II os trigonum, and the differences between the groups were statistically significant. There were no significant differences between type I and II os trigonum regarding posterior talofibular ligament (PTFL) abnormality, bone marrow edema, FHL tenosynovitis, and posterior synovitis. The study concluded that the os trigonum is a common cause of posterior ankle impingement, and type II os trigonum has a longer transverse diameter of the ossicle than type I.
Diagnostic Differentiation between Pancreatitis and Pancreatic Cancer: A Scoping Review
Authors: Madela Fusi , Ferndale Lucien , Aldous Colleen
Published: January 2024

Pancreatitis, encompassing acute and chronic forms, and pancreatic cancer pose significant challenges to the exocrine tissue of the pancreas. Recurrence rates and complications following acute pancreatitis episodes can lead to long-term risks, including diabetes mellitus. Chronic pancreatitis can develop in approximately 15% of cases, regardless of the initial episode’s severity. Alcohol-induced pancreatitis, idiopathic causes, cigarette smoking, and hereditary pancreatitis contribute to the progression to chronic pancreatitis. Chronic pancreatitis is associated with an increased risk of pancreatic cancer, with older age at onset and smoking identified as risk factors. This scoping review aims to synthesise recent publications (2017–2022) on the diagnostic differentiation between pancreatitis and pancreatic cancer while identifying knowledge gaps in the field. The review focuses on biomarkers and imaging techniques in individuals with pancreatitis and pancreatic cancer. Promising biomarkers such as faecal elastase-1 and specific chemokines offer non-invasive ways to assess pancreatic insufficiency and detect early biomarkers for chronic pancreatitis. Imaging techniques, including computed tomography (CT), magnetic resonance imaging (MRI), endoscopic ultrasound (EUS), and positron emission tomography (PET), aid in differentiating between chronic pancreatitis and pancreatic cancer. However, accurately distinguishing between the two conditions remains a challenge, particularly when a mass is present in the head of the pancreas. Several knowledge gaps persist despite advancements in understanding the association between pancreatitis and pancreatic cancer, including the correlation between histopathological grading systems, non-invasive imaging techniques, and biomarkers in chronic pancreatitis to determine the risk of progression to pancreatic cancer, as well as differentiating between the two conditions. Further research is necessary to enhance our understanding of these aspects, which can ultimately improve the diagnosis and management of pancreatitis and pancreatic cancer.
Time-Sequential Monitoring of the Early Mesothelial Reaction in the Pleura after Cryoinjury
Authors: Kim Taeyun , Chae Yu-Kyung , Nam Sung-Jin , Lee Haeyoung , Hwang Sang-Suk , Park Eun-Kee , Ahn Yeh-Chan , Oak Chulho
Published: January 2024

(1) Background: An early mesothelial reaction of the pleura, leading to fibrosis, has been reported in animals after chemical or heavy metal exposure. However, the visual monitoring of early time-sequential mesothelial reaction-associated cryoinjury has not been fully investigated. Therefore, this study aimed to evaluate and visualize the early mesothelial reactions seen following cryoinjury using rabbit pleura. (2) Methods: We monitored the early mesothelial reaction in rabbit pleurae after cryoinjury using optical coherence tomography (OCT), in real-time, which was then compared with pathological images. Due to the penetration limit of OCT, we made a thoracic window to image the parietal and visceral pleurae in vivo. We also used an innovative technique for capturing the microstructure in vivo, employing a computer-controlled intermittent iso-pressure breath hold to reduce respiratory motion, increasing the resolution of OCT. We organized three sample groups: the normal group, the sham group with just a thoracic window, and the experimental group with a thoracic window and cryotherapy. In the experimental group, localized cryoinjury was performed. The mesothelial cells at the level of pleura of the cryotherapy-injured site were visualized by OCT within the first 30 min and then again after 2 days at the same site. (3) Results: In the experimental group, focal thickening of the parietal pleura was observed at the site of cryoinjury using OCT after the first injury, and it was then confirmed pathologically as focal mesothelial cell proliferation. Two days after cryoinjury, diffuse mesothelial cell proliferation in the parietal pleura was noted on the reverse side around the cryoinjured site in the same rabbit. In the sham group, no pleural reaction was found. The OCT and pathological examinations revealed different patterns of mesothelial cell reactions between the parietal and visceral pleurae: the focal proliferation of mesothelial cells was found in the parietal pleura, while only a morphological change from flat cells to cuboidal cells and a thickened monolayer without proliferation of mesothelial cells were found in the visceral pleural. (4) Conclusions: An early mesothelial reaction occurs following cryoinjury to the parietal and visceral pleurae.
Periodontal Health Status in Adults Exposed to Tobacco Heating System Aerosol and Cigarette Smoke vs. Non-Smokers: A Cross-Sectional Study
Authors: Mišković Ivana , Kuiš Davor , Špalj Stjepan , Pupovac Aleksandar , Prpić Jelena
Published: January 2024

Tobacco heating systems (THS) are new products on the market, advertised as a less harmful alternative for smokers, in which tobacco is heated and not burned like in conventional cigarettes. This research explored the effect on periodontal tissues in contact with heating and burning tobacco residual products (smoke and tobacco). Methods: The sample included 66 subjects, patients of the Clinic of Dentistry in Rijeka, Croatia, aged 26–56 (median 38), 64% females. Three age- and gender-matched groups were formed (each N = 22): non-smokers, classic cigarettes smokers and THS smokers. Probing depth (PD) and clinical attachment loss (CAL) were primary research parameters. Results: Three groups differed in average PD and CAL (p ≤ 0.002), with cigarette smokers having the highest and non-smokers the lowest values (p ≤ 0.002). THS consumers generally had lower values of periodontal indices than smokers, but only CAL differed significantly (p = 0.011). Periodontal indices CAL and PD were worse in THS consumers than non-smokers, but they did not reach a level of statistical significance. Cigarette smoking was the only predictor of periodontitis (average CAL ≥ 4 mm) in logistic regression models, with an odds ratio of 4.7 (95% confidence interval 1.2–18.3; p = 0.027). Conclusions: Exposure to nicotine-containing aerosol of THS in adults has a less harmful effect on periodontal tissues, measurable through periodontal indices (PD and CAL), compared to burning tobacco of conventional cigarettes. THS, presented as an alternative product to classic cigarettes, also has a detrimental effect on the periodontium.
On the Nuisance Parameter Elimination Principle in Hypothesis Testing
Authors: Flórez Rivera Andrés Felipe , Esteves Luis Gustavo , Fossaluza Victor , de Bragança Pereira Carlos Alberto
Published: January 2024

The Non-Informative Nuisance Parameter Principle concerns the problem of how inferences about a parameter of interest should be made in the presence of nuisance parameters. The principle is examined in the context of the hypothesis testing problem. We prove that the mixed test obeys the principle for discrete sample spaces. We also show how adherence of the mixed test to the principle can make performance of the test much easier. These findings are illustrated with new solutions to well-known problems of testing hypotheses for count data.
Open Problems within Nonextensive Statistical Mechanics
Authors: Nelson Kenric P.
Published: January 2024

Nonextensive statistical mechanics has developed into an important framework for modeling the thermodynamics of complex systems and the information of complex signals. To mark the 80th birthday of the field’s founder, Constantino Tsallis, a review of open problems that can stimulate future research is provided. Over the thirty-year development of NSM, a variety of criticisms have been published ranging from questions about the justification for generalizing the entropy function to the interpretation of the generalizing parameter q. While these criticisms have been addressed in the past and the breadth of applications has demonstrated the utility of the NSM methodologies, this review provides insights into how the field can continue to improve the understanding and application of complex system models. The review starts by grounding q-statistics within scale-shape distributions and then frames a series of open problems for investigation. The open problems include using the degrees of freedom to quantify the difference between entropy and its generalization, clarifying the physical interpretation of the parameter q, improving the definition of the generalized product using multidimensional analysis, defining a generalized Fourier transform applicable to signal processing applications, and re-examining the normalization of nonextensive entropy. This review concludes with a proposal that the shape parameter is a candidate for defining the statistical complexity of a system.
Improving Black Entrepreneurship through Cannabis-Related Education
Authors: Reid Alicia E. , Crump Micah E. S. , Singh Robert P.
Published: January 2024

Economic inequality is a significant and growing issue in the U.S., particularly within Black communities. Improving Black entrepreneurship is critical to addressing the economic gaps. In this paper, we discuss a unique, newly established educational program focused on cannabis education at Medgar Evers College (MEC) in New York. One of the goals of the new program is to increase Black entrepreneurship in the rapidly growing cannabis industry. For decades, cannabis use and distribution were criminal offenses that led to the disproportionate incarceration of Black individuals compared to White individuals. As it is being decriminalized across the country, and medical and recreational use spreads, the tool formerly used for Black incarceration can now become a tool for advancing Black entrepreneurship. To work and/or operate a venture in the cannabis industry requires students to understand a range of issues and build a strong foundation of broad knowledge. We discuss the emerging curricula and illustrate some of the elements that will help spur successful new venture creation led by Black and minority entrepreneurs in this exciting new field. Some early successes and lessons learned are discussed, which can help other educational institutions looking to create or improve their own cannabis-related curricula.
Assessing Disparities in Predictive Modeling Outcomes for College Student Success: The Impact of Imputation Techniques on Model Performance and Fairness
Authors: Nezami Nazanin , Haghighat Parian , Gándara Denisa , Anahideh Hadis
Published: January 2024

The education sector has been quick to recognize the power of predictive analytics to enhance student success rates. However, there are challenges to widespread adoption, including the lack of accessibility and the potential perpetuation of inequalities. These challenges present in different stages of modeling, including data preparation, model development, and evaluation. These steps can introduce additional bias to the system if not appropriately performed. Substantial incompleteness in responses is a common problem in nationally representative education data at a large scale. This can lead to missing data and can potentially impact the representativeness and accuracy of the results. While many education-related studies address the challenges of missing data, little is known about the impact of handling missing values on the fairness of predictive outcomes in practice. In this paper, we aim to assess the disparities in predictive modeling outcomes for college student success and investigate the impact of imputation techniques on model performance and fairness using various notions. We conduct a prospective evaluation to provide a less biased estimation of future performance and fairness than an evaluation of historical data. Our comprehensive analysis of a real large-scale education dataset reveals key insights on modeling disparities and the impact of imputation techniques on the fairness of the predictive outcome under different testing scenarios. Our results indicate that imputation introduces bias if the testing set follows the historical distribution. However, if the injustice in society is addressed and, consequently, the upcoming batch of observations is equalized, the model would be less biased.
A Multidimensional Analysis of a High-Stakes English Listening Test: A Corpus-Based Approach
Authors: Tao Xuelian , Aryadoust Vahid
Published: January 2024

The Gaokao, also known as China’s national college entrance exam, is a high-stakes exam for nearly all Chinese students. English has been one of the three most important subjects for a long time, and listening plays an important role in the Gaokao English test. However, relatively little research has been conducted on local versions of Gaokao’s English listening tests. This study analyzed the linguistic features and corresponding functional dimensions of the three different text types in the Gaokao’s listening test, investigating whether the papers used in three major regions of China were differentiated in terms of the co-occurrence patterns of lexicogrammatical features and dimensions of the transcripts. A corpus consisting of 170 sets of test papers (134,913 words) covering 31 provinces and cities from 2000 to 2022 was analyzed using a multidimensional analysis wherein six exclusive dimensions were extracted. The results showed that there were meaningful differences across short conversations, long conversations, and monologues with regard to the six dimensions’ scores, and regions further had significant differences in three dimensions: Syntactic and Clausal Complexity, Oral versus Literate Discourse, and Procedural Discourse, while Time Period was not associated with any differences. Implications for language teaching and assessment are discussed.
Functional Characterization of Terpene Synthases from Masson Pine (Pinus massoniana) under Feeding of Monochamus alternatus Adults
Authors: Wen Quanmin , Chen Ruixu , Xu Tian , Hao Dejun
Published: January 2024

Conifers have evolved sophisticated terpenoid defenses for protection against herbivores and pathogens. Pinus massoniana Lamb. is the most widely distributed pioneer afforestation and resin tree species in China, but is seriously harmed by pine wilt disease. Monochamus alternatus is the main vector of pine wilt disease in China. Monoterpenes, sesquiterpenes and diterpenes, the main secondary defensive compounds of P. massoniana, are catalyzed by different terpene synthases (TPSs), which participate in the important defense pathways against external biotic and abiotic stresses. Here, we aimed to identify the terpene synthases (TPSs) in P. massoniana, responding to the feeding of M. alternatus, and to characterize the functions and products of the mono-TPSs. We identified six differentially expressed TPS genes in the P. massoniana fed upon by M. alternatus, including four mono-TPS and two sesqui-TPS genes. The functions of the four mono-TPSs were verified by analysis of the main product and by-products of these mono-TPSs. (+)-α-Pinene, (−)-α-pinene, and limonene were the major products of TPS (+)-α-pinene, TPS (−)-α-pinene, and TPS limonene, respectively, but TPS (−)-β-pinene only catalyzed a trace amount of (−)-β-pinene in the products. Our findings shed light on the potential relationships between the structure of terpene synthases and their corresponding products.
Preliminary Tests of a Hybrid Cable Splice (Synthetic–Metal) to Innovate Timber Harvesting in the Mediterranean Forestry Sector
Authors: Alcoverro Gerard , Raddi Adriano , Picchi Gianni
Published: January 2024

Forest operations in the Mediterranean basin are still largely based on lowly mechanized systems. In this context, hauling is generally performed with equipment deploying steel cables, such as winches on farm tractors or skidders. In the last decades, several efforts focused on the substitution of steel cables with synthetic rope to improve safety, comfort and productivity. Despite the expected benefits, these efforts were generally frustrated due to the higher cost and lower duration of synthetic cables. These are relevant constraints, particularly for Mediterranean forest companies, which feature a relatively low financial capacity. As a possible solution, the present study introduces a hybrid splice of steel and synthetic cables, merging the benefits of the two materials. For that purpose, several possible splicing solutions were tested. The most efficient splice proved capable of withstanding up to 7.6 t of tension in the laboratory. In the forest, it allowed the extraction of about 450 t with a skidder before breaking. On a farm tractor, it extracted over 700 t without failure. Preliminary tests and the positive feedback of the operators proved the potential of this solution. Further research is ongoing to create a stronger splice and reduce its diameter to allow its deployment in any type of winch.
Branch Elongation, Bud Durability, and Wind-Generated Crown Movement Associated with Crown Abrasion in Deciduous Trees
Authors: Clatterbuck Wayne K. , Brannon Tyler M. L. , Yost Edward C.
Published: January 2024

Trees that grow in close proximity to other trees are subject to crown and branch abrasion, causing mechanical injury. The loss of branch tips and buds through abrasion can affect the architecture and growth of tree crowns. This research quantifies the impacts of crown abrasion between neighboring trees of several deciduous species and how crown abrasion may influence stand dynamics. Tree interactions were evaluated during the dormant and growing seasons to determine how wind-generated movement affects crowns under foliated and un-foliated conditions. Branch elongation was measured in tree crowns where growth was both inhibited and uninhibited by adjacent trees. Bud durability was evaluated by growing season for species with determinate and indeterminate shoot growth forms using a pendulum impact tester. Crown movement during wind events was assessed by using three-axial accelerometers in the outermost points of tree crowns. Accelerometers logged the movement of branches in the tree crown. By using both the crown sway acceleration and associated bud durability and mass data, the possible force necessary to break or abrade buds and branches was calculated at different wind speeds. Branch elongation was greater for most species on the exposed side of the crown that was not affected by adjacent trees. Preformed buds from the determinate growth form were determined to have greater durability than sustained growth or indeterminant buds. Acceleration from wind gusts increased more rapidly as wind speed intensified in the growing season when leaves were on the tree than in the dormant season. This research suggests that crown abrasion contributes to the development of mixed species stands by reducing crown size and growth therefore allowing slower-growing species with determinant growth to stratify above faster growing trees with indeterminant growth.
Unraveling the Relative Contributions of Deterministic and Stochastic Processes in Shaping Species Community Assembly in a Floodplain and Shallow Hillslope System
Authors: Mendoza-Arroyo Gustavo Enrique , Canché-Solís René Efraín , Morón-Ríos Alejandro , González-Espinosa Mario , Méndez-Toribio Moisés
Published: January 2024

Understanding the process underlying species coexistence is crucial in ecology. This challenge is relevant in tree communities inhabiting contrasting abiotic conditions, such as lowland floodplain and shallow hillslope karstic systems. We examined the influence of topographic variables and spatial factors on the structure of tree communities in the karstic system in Calakmul, Mexico. We measured 7050 trees (diameter at breast height ≥ 3 cm) in 152 circular plots and generated seven topographic variables from a digital elevation model. We employed redundancy analysis and variance partitioning to test the effects of environmental and spatial factors on tree communities. In addition, we used the null Raup–Crick model to uncover the relative importance of the deterministic and stochastic processes driving community assembly. Our study revealed significant floristic distinction between seasonally flooded and upland forests. The topographic wetness index (TWI) contribution to explaining the floristic differentiation in the studied tree assemblages was greater than that of the other topography-related variables. The explanatory power of the environmental and spatial factors varied slightly between datasets. The null model indicated a predominant influence of deterministic over stochastic processes. Our findings reaffirm the role of seasonal flooding as an abiotic filter. Additionally, the TWI can serve to identify flood-prone conditions within shallow depressions. The preservation of adjacent seasonally flooded and upland forests is relevant for the maintenance of tree diversity in the karst of the Yucatan Peninsula, since flooding drives the distribution of species.
Comprehensive Response of Daily Transpiration from Armeniaca sibirica Plantations to Meteorological and Soil Moisture/Temperature Conditions on the Semi-Arid Loess Plateau, China
Authors: Han Xinsheng , Liu Guangquan , Xu Hao , Dong Liguo , Wang Xiao
Published: January 2024

Forest transpiration plays a vital role in the regional water budget and water supply security of the semi-arid Loess Plateau of northwest China. A thorough understanding and accurate predictions of the variation in the transpiration of forests with important tree species, e.g., Armeniaca sibirica (L.) Lam., are critical for land and water management. Owing to the extreme climatic seasonality and interannual variability, detailed information on the seasonal variation in the transpiration of Armeniaca sibirica plantations and its response to climatic and soil moisture/temperature fluctuations is limited. Therefore, in this study, the sap flux density and meteorological and soil moisture/temperature conditions were continuously monitored during the growing season (May to October) in 2019–2020. The results show the four following features: (1) The mean daily transpiration of the Armeniaca sibirica plantation was 0.31 mm·day−1; (2) the daily transpiration varied nonlinearly with increasing potential evapotranspiration (PET). Transpiration first increased rapidly until reaching the PET threshold of 4 mm·day−1 and then slowly increased within the PET range of 4–8.5 mm·day−1, but thereafter, it decreased slightly when PET exceeded 8.5 mm·day−1; (3) the daily forest transpiration varied with increasing relative extractable soil water content (REW) and soil temperature (ST) following a saturated exponential function; i.e., it first increased until reaching a threshold of 0.5 of REW or 14 °C of ST, but thereafter tended to stabilize; (4) models for estimating the daily forest transpiration were established. According to these models, PET had the greatest limiting effect (32.17%) on forest transpiration during the observation period, while REW and ST showed lower limits at 7.03% and 3.87%, respectively. The findings of this study are useful for understanding and managing the hydrological effects of forests in the semi-arid Loess Plateau as a typical dryland with seasonal droughts.
A New Forest Growing Stock Volume Estimation Model Based on AdaBoost and Random Forest Model
Authors: Wang Xiaorui , Zhang Chao , Qiang Zhenping , Xu Weiheng , Fan Jinming
Published: January 2024

Forest growing stock volume is a crucial indicator for assessing forest resources. However, contemporary machine learning models used in estimating forest growing stock volume often exhibit fluctuating precision and are confined to specific tree species, lacking universality. This limitation impedes their capacity to provide comprehensive forest survey services. This study designed a novel model for predicting forest growing stock volume named RF-Adaboost. The model represented the inaugural application of the Adaboost algorithm in estimating forest growing stock volume. Additionally, the authors innovatively refined the Adaboost algorithm by integrating Random Forest as its weak learner. To substantiate the model’s effectiveness, the authors designed three data combination schemes at different scales and conducted regression estimation using the RF-Adaboost model, traditional Random Forest, and Adaboost models, respectively. The results indicated that the RF-Adaboost model consistently outperforms others across various data schemes. Furthermore, utilizing a combined data scheme of remote sensing and Continuous Forest Inventory, the RF-Adaboost model demonstrated optimal performance in estimating forest growing stock volume (R2 = 0.81, RMSE = 7.08 m3/site, MAE = 3.36 m3, MAPE = 8%). Finally, the RF-Adaboost model exhibits greater universality, eliminating the need for strict differentiation between tree species. This research presented an efficient and cost-effective approach to estimate forest growing stock, addressing the challenges associated with conventional survey methods.