bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Granulocyte Colony-Stimulating Factor in Patients with a Large Anterior Wall Acute Myocardial Infarction to Prevent Left Ventricular Remodeling: A 10-Year Follow-Up of the RIGENERA Study
Authors: Leone Antonio Maria , D’Amario Domenico , Cannata Francesco , Graziani Francesca , Borovac Josip A. , Leone Giuseppe , De Stefano Valerio , Basile Eloisa , Siracusano Andrea , Galiuto Leonarda , Locorotondo Gabriella , Porto Italo , Vergallo Rocco , Canonico Francesco , Restivo Attilio , Rebuzzi Antonio Giuseppe , Crea Filippo
Published: April 2020

Background: the RIGENERA trial assessed the efficacy of granulocyte-colony stimulating factor (G-CSF) in the improvement of clinical outcomes in patients with severe acute myocardial infarction. However, there is no evidence available regarding the long-term safety and efficacy of this treatment. Methods: in order to evaluate the long-term effects on the incidence of major adverse events, on the symptom burden, on the quality of life and the mean life expectancy and on the left ventricular (LV) function, we performed a clinical and echocardiographic evaluation together with an assessment using the Minnesota Living with Heart Failure Questionnaire (MLHFQ) and the Seattle Heart Failure Model (SHFM) at 10-years follow-up, in the patients cohorts enrolled in the RIGENERA trial. Results: thirty-two patients were eligible for the prospective clinical and echocardiography analyses. A significant reduction in adverse LV remodeling was observed in G-CSF group compared to controls, 9% vs. 48% (p = 0.030). The New York Heart Association (NYHA) functional class was lower in G-CSF group vs. controls (p = 0.040), with lower burden of symptoms and higher quality of life (p = 0.049). The mean life expectancy was significantly higher in G-CSF group compared to controls (15 ± 4 years vs. 12 ± 4 years, p = 0.046. No difference was found in the incidence of major adverse events. Conclusions: this longest available follow-up on G-CSF treatment in patients with severe acute myocardial infarction (AMI) showed that this treatment was safe and associated with a reduction of adverse LV remodeling and higher quality of life, in comparison with standard-of-care treatment.
Clinical and Histopathological Characteristics of Cutaneous Lymphoid Hyperplasia: A Comparative Study According to Causative Factors
Authors: Choi Myoung Eun , Lee Keon Hee , Lim Dong Jun , Won Chong Hyun , Chang Sung Eun , Lee Mi Woo , Choi Jee Ho , Lee Woo Jin
Published: April 2020

Cutaneous lymphoid hyperplasia (CLH) is a heterogeneous type of reactive lymphocytic infiltration resembling cutaneous lymphoma clinically and histopathologically. Few studies describe the relationship between the causative agents and histopathological and immunohistochemical characteristics of CLH. We investigated the clinical and histopathological characteristics of 50 patients with cutaneous CLH and analyzed them according to causative factors and predominant cell types (B or T cells). We retrospectively reviewed medical records to identify causative agents, and histopathological and immunohistochemical features. The majority of infiltrating lymphocytes were T cells (60%). T cell-dominant CLH showed papuloplaque lesions, whereas B cell-dominant CLH lesions were nodular. The infiltration pattern differed between T and B cells. In terms of prognosis, B-cell-predominant lesions tended to respond better to treatment than T-cell-predominant lesions. Hair dyes tended to be associated with multiple CLH lesions in older patients. CLH lesions associated with drugs were located on the head and neck. Insect bites were likely to cause a solitary papular lesion. Histopathologically, infiltration depth was located more superficially than other causes and featured intense eosinophilic infiltration. Thus, our study demonstrated that CLH presents different clinicopathological features according to causative agents and predominant lymphocytic types.
Statistical Shape Analysis of Ascending Thoracic Aortic Aneurysm: Correlation between Shape and Biomechanical Descriptors
Authors: Cosentino Federica , Raffa Giuseppe M , Gentile Giovanni , Agnese Valentina , Bellavia Diego , Pilato Michele , Pasta Salvatore
Published: April 2020

An ascending thoracic aortic aneurysm (ATAA) is a heterogeneous disease showing different patterns of aortic dilatation and valve morphologies, each with distinct clinical course. This study aimed to explore the aortic morphology and the associations between shape and function in a population of ATAA, while further assessing novel risk models of aortic surgery not based on aortic size. Shape variability of n = 106 patients with ATAA and different valve morphologies (i.e., bicuspid versus tricuspid aortic valve) was estimated by statistical shape analysis (SSA) to compute a mean aortic shape and its deformation. Once the computational atlas was built, principal component analysis (PCA) allowed to reduce the complex ATAA anatomy to a few shape modes, which were correlated to shear stress and aortic strain, as determined by computational analysis. Findings demonstrated that shape modes are associated to specific morphological features of aneurysmal aorta as the vessel tortuosity and local bulging of the ATAA. A predictive model, built with principal shape modes of the ATAA wall, achieved better performance in stratifying surgically operated ATAAs versus monitored ATAAs, with respect to a baseline model using the maximum aortic diameter. Using current imaging resources, this study demonstrated the potential of SSA to investigate the association between shape and function in ATAAs, with the goal of developing a personalized approach for the treatment of the severity of aneurysmal aorta.
Pharmacogenomic (PGx) Counseling: Exploring Participant Questions about PGx Test Results
Authors: Schmidlen Tara , Sturm Amy , Scheinfeldt Laura
Published: April 2020

As pharmacogenomic (PGx) use in healthcare increases, a better understanding of patient needs will be necessary to guide PGx result delivery. The Coriell Personalized Medicine Collaborative (CPMC) is a prospective study investigating the utility of personalized medicine. Participants received online genetic risk reports for 27 potentially actionable complex diseases and 7 drug–gene pairs and could request free, telephone-based genetic counseling (GC). To explore the needs of individuals receiving PGx results, we conducted a retrospective qualitative review of inquiries from CPMC participants who requested counseling from March 2009 to February 2017. Eighty out of 690 (12%) total GC inquiries were focused on the discussion of PGx results, and six salient themes emerged: “general help”, “issues with drugs”, “relevant disease experience”, “what do I do now?”, “sharing results”, and “other drugs”. The number of reported medications with a corresponding PGx result and participant engagement were significantly associated with PGx GC requests (p < 0.01 and p < 0.02, respectively). Our work illustrates a range of questions raised by study participants receiving PGx test results, most of which were addressed by a genetic counselor with few requiring referrals to prescribing providers or pharmacists. These results further support a role for genetic counselors in the team-based approach to optimal PGx result delivery.
Evaluating the Community Land Record System in Monwabisi Park Informal Settlement in the Context of Hybrid Governance and Organisational Culture
Authors: Barry Michael , Kingwill Rosalie
Published: April 2020

The study examined the effectiveness of a community-operated land record system (CRS), a product of an evolutionary information system planning approach under hybrid governance arrangements in Monwabisi Park informal settlement in Cape Town. To structure the analysis, the authors adapted an analytical framework for analysing land registration effectiveness to community records systems. It serves as a tool for analysing, designing and managing similar information systems. The CRS is an element of a participatory planning and development project involving a triad: (a) community-based organisations (CBOs); (b) a non-governmental organisation (NGO), which has acted as a change agent, facilitator and resource provider; and (c) the City of Cape Town. The hybrid governance institutions comprised a set of local community and government protocols. Of further significance are the organisational cultures of the CBOs, and the NGO’s information system team differs markedly from that of most land registries. The researchers examined the CRS database and operations management, interviewed key-informants and interviewed shack residents door-to-door. The CRS was effective because residents used it and largely adhered to the associated documented community protocols to defend their tenure and to effect transactions in shacks. Further contributors were the NGO and CBOs continually managed the institutional and leadership dynamics relevant to the CRS, factors often ignored in similar projects.
Latitudinal Gradient in Urban Pressure and Socio-Environmental Quality: The “Peninsula Effect” in Italy
Authors: Romano Bernardino , Fiorini Lorena , Di Dato Chiara , Tomei Vanessa
Published: April 2020

The purpose of this work is to synthesize, for an international audience, certain fundamental elements that characterize the Italian peninsular territory, through the use of a biogeographical model known as the “peninsula effect” (PE). Just as biodiversity in peninsulas tends to change, diverging from the continental margin, so do some socio-economic and behavioral characteristics, for which it is possible to detect a progressive and indisputable variation depending on the distance from the continental mass. Through the use of 14 indicators, a survey was conducted on the peninsular sensitivity (which in Italy is also latitudinal) of as many phenomena. It obtained confirmation results for some of them, well known as problematic for the country, but contradictory results for others, such as those related to urban development. In the final part, the work raises a series of questions, also showing how peninsular Italy, and in particular Central–Southern Italy, is not penalized so dramatically by its geography and morphology as many political and scientific opinions suggest. The result is a very ambiguous image of Italy, in which the country appears undoubtedly uniform in some aspects, while the PE is very evident in others; it is probably still necessary to investigate, without relying on simplistic and misleading equations, the profound reasons for some phenomena that could be at the basis of less ephemeral rebalancing policies than those practiced in the past.
On the Role and Production of Polyhydroxybutyrate (PHB) in the Cyanobacterium Synechocystis sp. PCC 6803
Authors: Koch Moritz , Berendzen Kenneth W. , Forchhammer Karl
Published: April 2020

The cyanobacterium Synechocystis sp. PCC 6803 is known for producing polyhydroxybutyrate (PHB) under unbalanced nutrient conditions. Although many cyanobacteria produce PHB, its physiological relevance remains unknown, since previous studies concluded that PHB is redundant. In this work, we try to better understand the physiological conditions that are important for PHB synthesis. The accumulation of intracellular PHB was higher when the cyanobacterial cells were grown under an alternating day–night rhythm as compared to continuous light. In contrast to previous reports, a reduction of PHB was observed when the cells were grown under conditions of limited gas exchange. Since previous data showed that PHB is not required for the resuscitation from nitrogen starvation, a series of different abiotic stresses were applied to test if PHB is beneficial for its fitness. However, under none of the tested conditions did cells containing PHB show a fitness advantage compared to a PHB-free-mutant (ΔphaEC). Additionally, the distribution of PHB in single cells of a population Synechocystis cells was analyzed via fluorescence-activated cell sorting (FACS). The results showed a considerable degree of phenotypic heterogeneity at the single cell level concerning the content of PHB, which was consistent over several generations. These results improve our understanding about how and why Synechocystis synthesizes PHB and gives suggestions how to further increase its production for a biotechnological process.
Study of Drilling Process by Cooling Compressed Air in Reinforced Polyether-Ether-Ketone
Authors: Domingo Rosario , de Agustina Beatriz , Marín Marta María
Published: April 2020

This study is focused on the application of a cooling compressed air system in drilling processes; this environmentally friendly technique allows removing material at very low temperatures, approximately up to −22 °C in the cutting area. The main goals are to find the most improve cutting conditions with less energy consumption, for the drilling of reinforced polyether-ether-ketone with glass fiber at 30% (PEEK-GF30) with cooling compressed air by a Ranque-Hilsch vortex tube, and to find a balance between environmental conditions and adequate process performance. Drilling tests were carried out on plates of PEEK-GF30 to analyze the influence of cutting parameters and environmental temperature (–22, 0 and 22 °C) on variables such as thrust forces, energy and material removed rate by the use of statistical methods; analysis of variance, analysis of means, response surface, and desirability function were employed to identify the optimum region that provides the most improved values of the aforementioned variables. Drill bit diameter was also analyzed to determine the quality of drilled holes. During the drilling processes, force signals were detected by a piezoelectric dynamometer connected to multichannel amplifier and a pyrometer was used to control the temperature. The diameters of the drilled holes were measured by a coordinate measuring machine. Cooling compressed air can be considered an adequate technique to improve the results from an environmental and efficient perspective; in particular, the maximum desirability function was found at a spindle speed of 7000 rpm, a feedrate of 1 mm/rev and a temperature close to −22 °C.
Effects of Surface Treatments of Glass Fiber-Reinforced Post on Bond Strength to Root Dentine: A Systematic Review
Authors: Mishra Lora , Khan Abdul Samad , Velo Marilia Mattar de Amoedo Campos , Panda Saurav , Zavattini Angelo , Rizzante Fabio Antonio Piola , Arbildo Vega Heber Isac , Sauro Salvatore , Lukomska-Szymanska Monika
Published: April 2020

The objective of this systematic review was to determine the influence of surface treatment of glass fiber posts on bond strength to dentine. Laboratory studies were searched in MEDLINE, PubMed, Embase, PubMed Central, Scopus, and Web of Science search engine. All authors interdependently screened all identified articles for eligibility. The included studies were assessed for bias. Because of the considerable heterogeneity of the studies, a meta-analysis was not possible. Twelve articles were found eligible and included in the review. An assessment of the risk of bias in the included studies provided a result that classified the studies as low, medium, and high risk of bias. The available evidence indicated that the coronal region of the root canal bonded better to the glass fiber post than apical regions. Phosphoric acid, hydrogen peroxide, and silane application enhance post’s retentiveness. In light of the current evidence, surface treatment strategies increase the bond strength of glass fiber post to dentine. However, recommendations for standardized testing methods and reporting of future clinical studies are required to maintain clinically relevant information and to understand the effects of various surface treatment of glass fiber post and their bond strength with dentine walls of the root canal.
A Study for Improving Compressive Strength of Cementitious Mortar Utilizing Magnetic Water
Authors: Yousry Omar M. M. , Abdallah Metwally A. , Ghazy Mariam F. , Taman Mohamed H. , Kaloop Mosbeh R.
Published: April 2020

This research investigates the means to improve the compressive strength of mortar mixtures through using novel mixtures. These mixtures include magnetic water (MW) and fly ash (FA). MW was obtained by circulating tap water (TW) through a magnetic field. The magnetization duration was represented by the number of cycles, the content of FA was replaced with cement, and the super plasticizer percentage (SP) and the curing age were used and evaluated experimentally for producing the mortar mixtures. Mortar flow, crushing compressive strength, and ultrasonic pulse velocity (UPV) tests were applied to evaluate the performances of mixing characteristics. The results demonstrate that the MW-treated mortar mixtures show higher compression strength results than those prepared by TW. The compressive strength was increased up to 60% with 150 cycles, a dose of 0.5% of SP and no FA content at the age of 56 days. The dose of SP can be cut down by a maximum of 40% to 50% in cementitious mortar. the workability was enhanced by a percentage of 70%.
Supported Evacuation for Disaster Relief through Lexicographic Goal Programming
Authors: Flores Inmaculada , Ortuño M. Teresa , Tirado Gregorio , Vitoriano Begoña
Published: April 2020

Disasters have been striking human-beings from the beginning of history and their management is a global concern of the international community. Minimizing the impact and consequences of these disasters, both natural and human-made, involves many decision and logistic processes that should be optimized. A crucial logistic problem is the evacuation of the affected population, and the focus of this paper is the planning of supported evacuation of vulnerable people to safe places when necessary. A lexicographic goal programming model for supported evacuation is proposed, whose main novelties are the classification of potential evacuees according to their health condition, so that they can be treated accordingly; the introduction of dynamism regarding the arrival of potential evacuees to the pickup points, according to their own susceptibility about the disaster and the joint consideration of objectives such us number of evacuated people, operation time and cost, among which no trade-off is possible. The performance of the proposed model is evaluated through a realistic case study regarding the earthquake and tsunami that hit Palu (Indonesia) in September 2018.
Hemicranial Cough-Induced Headache as a First Symptom of a Carotid-Cavernous Fistula-Case Report
Authors: Simić Svetlana , Radmilo Ljiljana , Villar José R. , Kopitović Aleksandar , Simić Dragan
Published: April 2020

Background and objectives: Spontaneous carotid-cavernous fistulas (CCFs) are rare, and they may be caused by an aneurysm rupture. Materials and Methods: A case of a man hospitalized for high-intensity hemicranial headache with sudden cough onset as part of an upper respiratory tract infection is presented. The pain was of a pulsating character, localized on the right, behind the eye, followed by nausea and vomiting. Neurological finding registered a wider rima oculi to the right and slight neck rigidity. Laboratory findings detected a mild leukocytosis with neutrophil predominance, while cytobiochemical findings of CSF and a computerized tomography (CT) scan of the endocranium were normal. Results: Magnetic resonance imaging (MRI) angiography indicated the presence of a carotid cavernous fistula with a pseudoaneurysm to the right. Digital subtraction angiography (DSA) was performed to confirm the existence of the fistula. The planned artificial embolization was not performed because a complete occlusion of the fistula occurred during angiographic examination. Patient was discharged without subjective complaints and with normal neurological findings. Conclusions: Hemicranial cough-induced headache may be the first sign of carotid cavernous fistula, which was resolved by a spontaneous thrombosis in preparation for artificial embolization.
Forming Complex Graded and Homogeneous Components by Joining Simple Presintered Parts of TRIP-Matrix Composite through Powder Forging
Authors: Kirschner Markus , Martin Stefan , Guk Sergey , Prahl Ulrich , Kawalla Rudolf
Published: April 2020

The ability to fabricate complex graded structures would be a significant step towards the manufacturing of material systems with properties tailored to individual applications. While powder metallurgy has had some success in this regard, it requires that the semi-finished products be exactly similar to the final component. However, it is significantly cheaper to produce simple, semi-finished products and then join them to form complex components with the desired graded structure through powder forging and simultaneous compaction. It is also essential that the graded structure of the semi-finished products is retained during the forming process. In this study, pre-sintered cylindrical semi-finished products consisting of identical homogeneous layers as well as graded components consisting of non-identical homogeneous layers were joined using powder forging at 1100 °C. The microstructures and densities as well as the mechanical properties of the final components were investigated. It was observed that, upon compaction, the components formed solid structures, in which the reinforcing ZrO2 particles were completely integrated within the transformation-induced plasticity steel matrix. Finally, it was confirmed that the graded structure of the semi-finished products was retained in the final components.
Effect of Tool Offset on the Microstructure and Properties of AA6061/AZ31B Friction Stir Welding Joints
Authors: Liu Huachen , Chen Yikun , Yao Zhenhua , Luo Feixiang
Published: April 2020

Friction stir welding was carried out on AA6061/AZ31B alloy, and the influence of tool offset on microstructures and mechanical properties of the joints was studied. The results showed that preferred properties were obtained when Mg was placed in the Advancing Side (AS) and offset was positioned into Mg. The distribution of Al3Mg2 and Al12Mg17 intermetallic compounds (IMCs) could be improved with offset in a certain range. The tensile strength of the joints was elevated with the increase of the offset due to the superior distribution and diminished size of IMCs, and when the offset was 1.5 mm, the joint strength reached the maximum value of 107 MPa. The microhardness of the stirring zone decreased with the increased offset.
Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor
Authors: Kim Kyung Ho , Lee Sang Hun , Seo Sung Eun , Bae Joonwon , Park Seon Joo , Kwon Oh Seok
Published: April 2020

Stress biomarkers such as hormones and neurotransmitters in bodily fluids can indicate an individual’s physical and mental state, as well as influence their quality of life and health. Thus, sensitive and rapid detection of stress biomarkers (e.g., cortisol) is important for management of various diseases with harmful symptoms, including post-traumatic stress disorder and depression. Here, we describe rapid and sensitive cortisol detection based on a conducting polymer (CP) nanotube (NT) field-effect transistor (FET) platform. The synthesized polypyrrole (PPy) NT was functionalized with the cortisol antibody immunoglobulin G (IgG) for the sensitive and specific detection of cortisol hormone. The anti-cortisol IgG was covalently attached to a basal plane of PPy NT through an amide bond between the carboxyl group of PPy NT and the amino group of anti-cortisol IgG. The resulting field-effect transistor-type biosensor was utilized to evaluate various cortisol concentrations. Cortisol was sensitively measured to a detection limit of 2.7 × 10−10 M (100 pg/mL), with a dynamic range of 2.7 × 10−10 to 10−7 M; it exhibited rapid responses (<5 s). We believe that our approach can serve as an alternative to time-consuming and labor-intensive health questionnaires; it can also be used for diagnosis of underlying stress-related disorders.
Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps
Authors: Nagai Moeto , Kato Keita , Soga Satoshi , Santra Tuhin Subhra , Shibata Takayuki
Published: April 2020

High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 Vpp, and VDC: −4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation.
Rheology of a Dilute Suspension of Aggregates in Shear-Thinning Fluids
Authors: Trofa Marco , D’Avino Gaetano
Published: April 2020

The prediction of the viscosity of suspensions is of fundamental importance in several fields. Most of the available studies have been focused on particles with simple shapes, for example, spheres or spheroids. In this work, we study the viscosity of a dilute suspension of fractal-shape aggregates suspended in a shear-thinning fluid by direct numerical simulations. The suspending fluid is modeled by the power-law constitutive equation. For each morphology, a map of particle angular velocities is obtained by solving the governing equations for several particle orientations. The map is used to integrate the kinematic equation for the orientation vectors and reconstruct the aggregate orientational dynamics. The intrinsic viscosity is computed by a homogenization procedure along the particle orbits. In agreement with previous results on Newtonian suspensions, the intrinsic viscosity, averaged over different initial orientations and aggregate morphologies characterized by the same fractal parameters, decreases by increasing the fractal dimension, that is, from rod-like to spherical-like aggregates. Shear-thinning further reduces the intrinsic viscosity showing a linear dependence with the flow index in the investigated range. The intrinsic viscosity can be properly scaled with respect to the number of primary particles and the flow index to obtain a single curve as a function of the fractal dimension.
Aesculus hippocastanum L. Extract Does Not Induce Fibroblast to Myofibroblast Conversion but Increases Extracellular Matrix Production In Vitro Leading to Increased Wound Tensile Strength in Rats
Authors: Kováč Ivan , Melegová Nikola , Čoma Matúš , Takáč Peter , Kováčová Katarína , Hollý Martin , Ďurkáč Ján , Urban Lukáš , Gurbáľová Miriam , Švajdlenka Emil , Mojžišová Gabriela , Zajíček Robert , Szabo Pavol , Mučaji Pavel , Gál Peter
Published: April 2020

The ability of horse chestnut extract (HCE) to induce contraction force in fibroblasts, a process with remarkable significance in skin repair, motivated us to evaluate its wound healing potential in a series of experiments. In the in vitro study of the ability of human dermal fibroblasts to form myofibroblast-like cells was evaluated at the protein level (Western blot and immunofluorescence). The in vivo study was conducted on male Sprague-Dawley rats with inflicted wounds (one open circular and one sutured incision) on their backs. Rats were topically treated with two tested HCE concentrations (0.1% and 1%) or sterile water. The control group remained untreated. The incisions were processed for wound tensile strength (TS) measurement whereas the open wounds were subjected to histological examination. On the in vitro level the HCE extract induced fibronectin-rich extracellular matrix formation, but did not induced α-smooth muscle actin (SMA) expression in dermal fibroblasts. The animal study revealed that HCE increased wound TS and improved collagen organization. In conclusion, the direct comparison of both basic wound models demonstrated that the healing was significantly increased following HCE, thus this extract may be found useful to improve healing of acute wounds. Nevertheless, the use of an experimental rat model warrants a direct extrapolation to the human clinical situation.
Simultaneous Analysis of Fenthion and Its Five Metabolites in Produce Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
Authors: Lee Jonghwa , Kim Jeong-Han
Published: April 2020

A simultaneous analytical method for the organophosphorus insecticide fenthion and its five metabolites (fenthion oxon, fenthion oxon sulfoxide, fenthion oxon sulfone, fenthion sulfoxide, and fenthion sulfone) was developed based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Five matrices (brown rice, chili pepper, orange, potato, and soybean) were selected to validate the method. The target compounds were analyzed using positive electrospray ionization in the multiple reaction monitoring mode. For the best sensitivity in regard to the detector response, water and methanol containing formic acid (0.1%) were selected as the mobile phase. The optimum extraction efficiency was obtained through a citrate-buffered QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. Recovery tests were carried out at three spiking levels (n = 3). At all fortification levels, the accuracy and precision results were between 70% and 120% with a relative standard deviation of ≤15%. The limit of quantitation was 0.01 mg/kg, and the correlation coefficients (r2) of the matrix-matched calibration curves were >0.99. Significant signal suppression in the detector responses were observed for all matrices, suggesting that a compensation method, such as matrix-matched calibration, is required to provide accurate quantitative results. The applicability of the presented method was confirmed for the simultaneous analysis of fenthion and its metabolites in various crops.
Depolarization Ratios of Methane Raman Bands as a Function of Pressure
Authors: Petrov Dmitry
Published: April 2020

In this work, we measured the intensities of Q-branches of the ν1, ν2 and ν3 bands in the polarized and depolarized methane Raman spectra in the pressure range of 1–60 atm. It was established that the pressure dependence of depolarization ratios of the ν2 and ν3 bands are negligible. In turn, the depolarization ratio of the ν1 band increases with increasing pressure and reaches approximately 0.0045 at 60 atm. These data are more precise than previously published ones because ν1 band intensities were determined taking into account the contribution of overlapping lines of ν3 band. The presented data will be useful in calculating the methane polarizabilities at high pressure, as well as in calculating methane Raman spectra for measuring the natural gas composition using Raman spectroscopy.
Cyclic Synthetic Peroxides Inhibit Growth of Entomopathogenic Fungus Ascosphaera apis without Toxic Effect on Bumblebees
Authors: Yaremenko Ivan A. , Syromyatnikov Mikhail Y. , Radulov Peter S. , Belyakova Yulia Yu. , Fomenkov Dmitriy I. , Popov Vasily N. , Terent’ev Alexander O.
Published: April 2020

In recent years, the number of pollinators in the world has significantly decreased. A possible reason for this is the toxic effects of agrochemicals reducing the immunity of insects that leads to their increased susceptibility to pathogens. Ascosphaera apis is a dangerous entomopathogenic fungus, afflicting both honeybees and bumblebees. We investigated fungicide activity of cyclic synthetic peroxides against A. apis isolated from Bombus terrestris L. The peroxides exhibited high mycelium growth inhibition of A. apis up to 94–100% at concentration 30 mg/L. EC50 values were determined for the most active peroxides. Two peroxides showed higher antifungal activity against A. apis than the commercial fungicide Triadimefon. The studied peroxides did not reduce the ability of bumblebees to fly and did not lead to the death of bumblebees. A new field of application for peroxides was disclosed.
Development and In Vitro and In Vivo Evaluation of Microspheres Containing Sodium N-[8-(2-hydroxybenzoyl)amino]caprylate for the Oral Delivery of Berberine Hydrochloride
Authors: Li Ying , Zhu Chunyan
Published: April 2020

Microspheres containing absorption enhancer (sodium N-[8-(2-hydroxybenzoyl)amino]caprylate, SNAC) were developed to enhance the oral bioavailability of berberine hydrochloride (BER) with poor intestinal membrane permeability. Microspheres were prepared and characterized by particle size measurements, scanning electron microscopy, differential scanning calorimetry, BER payload and release, Caco-2 cell monolayer transport, and rat pharmacokinetics. The microspheres were spherical and had uniform size, high encapsulation efficiency and high loading capacity. In vitro release studies showed that BER-loaded microspheres had good sustained release characteristics. The Caco-2 cell monolayer transport study proved that SNAC could significantly enhance permeability of BER 2–3-fold. Pharmacokinetic studies demonstrated a 9.87-fold increase in area under the curve (AUC) of BER mixed with SNAC and a 14.14-fold increase in AUC of microspheres compared with BER alone. These findings indicate that SNAC is a promising absorption enhancer for oral delivery of BER in the form of both solution and microspheres.
Biophenolic Compounds Influence the In-Mouth Perceived Intensity of Virgin Olive Oil Flavours and Off-Flavours
Authors: Genovese Alessandro , Mondola Ferdinando , Paduano Antonello , Sacchi Raffaele
Published: April 2020

In this study, the influence of phenolic compounds on the sensory scores attributed to extra virgin olive oil (EVOO) by panel test was investigated. Two model olive oils (MOOs) with identical concentrations of volatile compounds, differing only in the amount of biophenols (297 vs. 511 mg kg−1), were analysed by two official panels and by SPME-GC/MS. Six other MOOs set up by the two previous models were also tested and analysed. They were formulated separately with the addition of three off-flavours (‘rancid’, ‘winey–vinegary’ and ‘fusty–muddy’). While high levels of EVOO phenolic compounds did not produce any effect on the headspace concentration of volatile compounds, they did affect the scores of both positive and negative sensory attributes of EVOO, due to the well-known in-mouth interactions between EVOO phenols, saliva and volatile compounds. In particular, a decrease of about 39% in the positive fruity score was found in the presence of a higher concentration of phenols. Regarding EVOO off-flavours, the higher level of phenolic compounds decreased by about 23% the score of ‘fusty–muddy’ defect and increased the score of ‘winey–vinegary’ defect about 733%. No important effect of EVOO phenolics on the perceived intensity of the ‘rancid’ defect was found. These findings could be helpful in explaining some discrepancies of panel test responses observed during extra virgin olive oil shelf life.
Concise Synthesis of Catechin Metabolites 5-(3′,4′-Dihydroxyphenyl)-γ-valerolactones (DHPV) in Optically Pure Form and Their Stereochemical Effects on Skin Wrinkle-Reducing Activities
Authors: Hur Joonseong , Kim A-Ram , Kim Hyun Su , Lim Changjin , Kim Taewoo , Kim Tae-Aug , Sim Jaehoon , Suh Young-Ger
Published: April 2020

A concise and scalable synthetic route for optically pure (4S) and (4R)-5-(3′,4′-dihydroxyphenyl)-γ-valerolactones (DHPVs), catechin metabolites, has been developed via the efficient construction of a γ-valerolactone moiety from hexenol. Noticeably, the different skin wrinkle-reducing activities of each metabolite were revealed via our unique syntheses of DHPVs in an enantiomerically pure form.
Qualitative Phytochemical Fingerprint and Network Pharmacology Investigation of Achyranthes aspera Linn. Extracts
Authors: Sinan Kouadio Ibrahime , Zengin Gokhan , Zheleva-Dimitrova Dimitrina , Etienne Ouattara Katinan , Fawzi Mahomoodally Mohamad , Bouyahya Abdelhakim , Lobine Devina , Chiavaroli Annalisa , Ferrante Claudio , Menghini Luigi , Recinella Lucia , Brunetti Luigi , Leone Sheila , Orlando Giustino
Published: April 2020

Achyranthes aspera Linn. (Amaranthaceae), commonly known as the Prickly Chaff flower, is used as herbal medicine in the Ivorian’s culture, Africa. Nonetheless, there is currently a paucity of scientific information on A. aspera from the Ivory Coast. Herein, the antioxidant activity of A. aspera extracts (methanol, dichloromethane, ethyl acetate and infusion) as well as the enzymatic inhibitory potentials towards key enzymes in human diseases, namely Alzheimer’s disease, (cholinesterases: AchE and BChE), type 2 diabetes (α-glucosidase and α-amylase) and hyperpigmentation (tyrosinase) were assessed. The total phenolic (TPC) and flavonoid (TFC) content was determined using colorimetric methods and the individual compounds were characterized using ultra-high performance liquid chromatography coupled with hybrid quadrupole-Orbitrap high resolution mass spectrometry (UHPLC-HRMS). Furthermore, a network pharmacology analysis was conducted to predict putative targets of identified phenolic compounds. The highest TPC was observed in the infused extract (28.86 ± 0.12 mg GAE/g), while the dichloromethane extract (38.48 ± 1.48 mg RE/g) showed the highest level of TFC. UHPLC-HRMS analysis has revealed an abundance of fatty acids, flavonoids, phenols and acylquinic acids. Among tested extracts, the infused extract displayed the highest free radical quenching, reducing and metal-chelating ability. The extracts (except infusion) were effective as enzyme inhibitors against AChE, while only methanolic and infused extracts showed noteworthy anti-BChE effects. The methanolic extract showed a remarkable antityrosinase effect (56.24 ± 5.05 mg KAE/g), as well. Modest to moderate inhibitory activity was observed against α-amylase (all extracts) and α-glucosidase (only dichloromethane extract). Finally, the network pharmacology analysis suggested the carbonic anhydrase II enzyme as a putative target for explaining, at least in part, the traditional use of A. aspera preparations as diuretic and blood clotting agent. Data amassed herein tend to validate the use of A. aspera in traditional medicine, as well as act as a stepping stone for further studies in the quest for novel phytopharmaceuticals. In this context, it is desirable that this study will contribute to the validation of the traditional uses of this plant in the African herbal medicine, and to the valorization of the whole chain production of A. aspera, as a local and sustainable botanical resource.
Tailoring the Properties of Thermo-Compressed Polylactide Films for Food Packaging Applications by Individual and Combined Additions of Lactic Acid Oligomer and Halloysite Nanotubes
Authors: Rojas-Lema Sandra , Quiles-Carrillo Luis , Garcia-Garcia Daniel , Melendez-Rodriguez Beatriz , Balart Rafael , Torres-Giner Sergio
Published: April 2020

In this work, films of polylactide (PLA) prepared by extrusion and thermo-compression were plasticized with oligomer of lactic acid (OLA) at contents of 5, 10, and 20 wt%. The PLA sample containing 20 wt% of OLA was also reinforced with 3, 6, and 9 parts per hundred resin (phr) of halloysite nanotubes (HNTs) to increase the mechanical strength and thermal stability of the films. Prior to melt mixing, ultrasound-assisted dispersion of the nanoclays in OLA was carried out at 100 °C to promote the HNTs dispersion in PLA and the resultant films were characterized with the aim to ascertain their potential in food packaging. It was observed that either the individual addition of OLA or combined with 3 phr of HNTs did not significantly affect the optical properties of the PLA films, whereas higher nanoclay contents reduced lightness and induced certain green and blue tonalities. The addition of 20 wt% of OLA increased ductility of the PLA film by nearly 75% and also decreased the glass transition temperature (Tg) by over 18 °C. The incorporation of 3 phr of HNTs into the OLA-containing PLA films delayed thermal degradation by 7 °C and additionally reduced the permeabilities to water and limonene vapors by approximately 8% and 47%, respectively. Interestingly, the highest barrier performance was attained for the unfilled PLA film plasticized with 10 wt% of OLA, which was attributed to a crystallinity increase and an effect of “antiplasticization”. However, loadings of 6 and 9 phr of HNTs resulted in the formation of small aggregates that impaired the performance of the blend films. The here-attained results demonstrates that the properties of ternary systems of PLA/OLA/HNTs can be tuned when the plasticizer and nanofiller contents are carefully chosen and the resultant nanocomposite films can be proposed as a bio-sourced alternative for compostable packaging applications.
PERSEL, a Ready-to-Use PERsonality-Based User SELection Tool to Maximize User Experience Redesign Effectiveness
Authors: Filippi Stefano
Published: April 2020

Some literature has already demonstrated the widespread influence of human personality on product design. Nevertheless, most of the existing user experience (UX) design methods and tools do not fully exploit knowledge about user personality in selecting the best participants to maximize the effectiveness of the design efforts. This research tries to fill the gap by introducing PERSEL, the ready-to-use PERsonality-based SELector. PERSEL is a Microsoft Excel workbook, free to download, which allows expression of the objectives (needs) and assessment of the user personality; in turn, PERSEL suggests the best users to be involved in UX redesign activities and in what way, in order to get solutions answering to the needs in the best possible way. A comparison of the solutions generated by the first adoption of PERSEL in the field with those coming from the involvement of users selected without obeying any specific criterion, begins validating the research results, mainly in terms of PERSEL functioning and effectiveness.
Conductive Atomic Force Microscopy of Semiconducting Transition Metal Dichalcogenides and Heterostructures
Authors: Giannazzo Filippo , Schilirò Emanuela , Greco Giuseppe , Roccaforte Fabrizio
Published: April 2020

Semiconducting transition metal dichalcogenides (TMDs) are promising materials for future electronic and optoelectronic applications. However, their electronic properties are strongly affected by peculiar nanoscale defects/inhomogeneities (point or complex defects, thickness fluctuations, grain boundaries, etc.), which are intrinsic of these materials or introduced during device fabrication processes. This paper reviews recent applications of conductive atomic force microscopy (C-AFM) to the investigation of nanoscale transport properties in TMDs, discussing the implications of the local phenomena in the overall behavior of TMD-based devices. Nanoscale resolution current spectroscopy and mapping by C-AFM provided information on the Schottky barrier uniformity and shed light on the mechanisms responsible for the Fermi level pinning commonly observed at metal/TMD interfaces. Methods for nanoscale tailoring of the Schottky barrier in MoS2 for the realization of ambipolar transistors are also illustrated. Experiments on local conductivity mapping in monolayer MoS2 grown by chemical vapor deposition (CVD) on SiO2 substrates are discussed, providing a direct evidence of the resistance associated to the grain boundaries (GBs) between MoS2 domains. Finally, C-AFM provided an insight into the current transport phenomena in TMD-based heterostructures, including lateral heterojunctions observed within MoxW1–xSe2 alloys, and vertical heterostructures made by van der Waals stacking of different TMDs (e.g., MoS2/WSe2) or by CVD growth of TMDs on bulk semiconductors.
A Nano-Rattle SnO2@carbon Composite Anode Material for High-Energy Li-ion Batteries by Melt Diffusion Impregnation
Authors: Maharajan Sivarajakumar , Kwon Nam Hee , Brodard Pierre , Fromm Katharina M.
Published: April 2020

The huge volume expansion in Sn-based alloy anode materials (up to 360%) leads to a dramatic mechanical stress and breaking of particles, resulting in the loss of conductivity and thereby capacity fading. To overcome this issue, SnO2@C nano-rattle composites based on <10 nm SnO2 nanoparticles in and on porous amorphous carbon spheres were synthesized using a silica template and tin melting diffusion method. Such SnO2@C nano-rattle composite electrodes provided two electrochemical processes: a partially reversible process of the SnO2 reduction to metallic Sn at 0.8 V vs. Li+/Li and a reversible process of alloying/dealloying of LixSny at 0.5 V vs. Li+/Li. Good performance could be achieved by controlling the particle sizes of SnO2 and carbon, the pore size of carbon, and the distribution of SnO2 nanoparticles on the carbon shells. Finally, the areal capacity of SnO2@C prepared by the melt diffusion process was increased due to the higher loading of SnO2 nanoparticles into the hollow carbon spheres, as compared with Sn impregnation by a reducing agent.
Multi-Frequency Resonance Behaviour of a Si Fractal NEMS Resonator
Authors: Tzanov Vassil , Llobet Jordi , Torres Francesc , Perez-Murano Francesc , Barniol Nuria
Published: April 2020

Novel Si-based nanosize mechanical resonator has been top-down fabricated. The shape of the resonating body has been numerically derived and consists of seven star-polygons that form a fractal structure. The actual resonator is defined by focused ion-beam implantation on a SOI wafer where its 18 vertices are clamped to nanopillars. The structure is suspended over a 10 μm trench and has width of 12 μm. Its thickness of 0.040 μm is defined by the fabrication process and prescribes Young’s modulus of 76 GPa which is significantly lower than the value of the bulk material. The resonator is excited by the bottom Si-layer and the interferometric characterisation confirms broadband frequency response with quality factors of over 800 for several peaks between 2 MHz and 16 MHz. COMSOL FEM software has been used to vary material properties and residual stress in order to fit the eigenfrequencies of the model with the resonance peaks detected experimentally. Further use of the model shows how the symmetry of the device affects the frequency spectrum. Also, by using the FEM model, the possibility for an electrical read out of the device was tested. The experimental measurements and simulations proved that the device can resonate at many different excitation frequencies allowing multiple operational bands. The size, and the power needed for actuation are comparable with the ones of single beam resonator while the fractal structure allows much larger area for functionalisation.