bluuebunny/crossref_metadata_2025 huggingface dataset:
Laser adaptive processing technology for multilayer dissimilar materials
Authors: Yang Guiyang , Yan Hangrui , Liu Le , Wang Quanji , Chen Qiaodan , Xiong Wei , Deng Leimin , Liu Lei
Published: August 2023

We report a laser adaptive processing technology (LAPT) for the selective removal of Cu/Al multilayer dissimilar materials. Using the wavelength range and intensity distribution of the characteristic spectrum, the properties and content of multilayer dissimilar materials can be analyzed online based on laser-induced breakdown spectroscopy. The traditional low-speed spectral detection mode was transformed into a high-speed photoelectric detection method by using a scheme consisting of a bandpass filter with an avalanche photodetector (APD), and the in situ online detection of a 30 ns, 40 kHz high-frequency pulse signal during laser scanning was realized. Combined with a field programmable gate array (FPGA) digital control unit, online feedback and closed-loop control were achieved at the kHz level, and the adaptive intelligent control of material interfaces and laser processing parameters was achieved. This excellently demonstrated the feasibility and flexibility of LAPT for processing arbitrary multilayer dissimilar materials.
Optics Continuum recognizes the best paper prize winners: editorial
Authors: Omatsu Takashige , Vartianen Erik
Published: August 2023

The Editor-in-Chief and Deputy Editor of Optics Continuum announce the recipients of a new Best Paper Prize.
Real-timing processing of fiber bundle endomicroscopy images in Python using PyFibreBundle
Authors: Hughes Michael
Published: August 2023

Fiber imaging bundles allow the transfer of optical images from place-to-place along narrow and flexible conduits. Traditionally used extensively in medical endoscopy, bundles are now finding new applications in endoscopic microscopy and other emerging techniques. PyFibreBundle is an open source Python package for fast processing of images acquired through imaging bundles. This includes detection and removal of the fiber core pattern by filtering or interpolation, and application of background and flat-field corrections. It also allows images to be stitched together to create mosaics, and resolution to be improved by combining multiple shifted images. This article describes the technical implementation of PyFibreBundle and provides example results from three endomicroscopy imaging systems: color transmission, monochrome transmission and confocal fluorescence. This allows various processing options to be compared quantitatively and qualitatively, and benchmarking demonstrates that PyFibreBundle can achieve state-of-the-art performance in an open-source package. The article demonstrates core removal by interpolation and mosaicing at over 100 fps, real-time multi-frame resolution enhancement, and the first demonstration of endomicroscopy image capture and processing via a Raspberry Pi single board computer. This demonstrates that PyFibreBundle is potentially a valuable tool for the development of low-cost, high-performance fiber bundle imaging systems.
Assessing awareness and use of HIV self-testing kits after the introduction of a community-based HIV self-testing programme among men who have sex with men in Kenya
Authors: Shaw Souradet Y. , Leung Stella , Isac Shajy , Musyoki Helgar , Mugambi Mary , Kioko Japheth , Musimbi Janet , Olango Kennedy , Kuria Samuel , Ongaro Martin K. , Walimbwa Jeffrey , Melon Memory , Emmanuel Faran , Moses Stephen , Blanchard James F. , Pickles Michael , Lazarus Lisa , Lorway Robert R. , Becker Marissa L. , Mishra Sharmistha , Bhattacharjee Parinita
Published: August 2023

Men who have sex with men (MSM) bear a disproportionate burden of new HIV infections in Kenya, while experiencing discrimination, leading to suboptimal levels of HIV care. HIV self-testing (HIVST) is a tool to increase HIV screening and earlier diagnosis; however, questions remain regarding how best to scale-up HIVST to MSM in Kenya. The main objective of this study was to examine changes in knowledge and use of HIVST after implementation of a community-led HIVST project. Participants were MSM recruited from Kisumu, Mombasa, and Kiambu counties. Data were collected from two rounds (Round 1: 2019; Round 2: 2020) of serial cross-sectional integrated biological and behavioural assessments (IBBA), pre-, and post-project implementation. Two main outcomes were measured: 1) whether the respondent had ever heard of HIVST; and 2) whether they had ever used HIVST kits. Changes in outcomes between IBBA rounds were examined using modified multivariable Poisson regression models; adjusted prevalence ratios (aPR) and 95% confidence intervals (95% CI) are reported. A total of 2,328 respondents were included in main analyses. The proportion of respondents who had heard of HIVST increased from 75% in Round 1 to 94% in Round 2 (aPR: 1.2, 95% CI: 1.2–1.3), while those reporting using an HIVST kit increased from 20% to 53% (aPR: 2.3, 95% CI: 2.0–2.6). Higher levels of education and HIV programme awareness were associated with both outcomes. Awareness and use of HIVST kits increased after implementation of a community-led HIVST implementation project, demonstrating the importance of integration with existing community groups.
Failure to receive prescribed imaging is associated with increased early mortality after injury in Cameroon
Authors: Driban Matthew , Dissak-Delon Fanny N. , Carvalho Melissa , Mbianyor Mbiarikai , Etoundi-Mballa Georges A. , Kingue Thompson , Njock Richard L. , Nkusu Daniel N. , Tsiagadigui Jean-Gustave , Puyana Juan C. , Juillard Catherine , Chichom-Mefire Alain , Christie S. Ariane
Published: August 2023

Despite having the highest rates of injury-related mortality in the world, trauma system capacity in sub-Saharan Africa remains underdeveloped. One barrier to prompt diagnosis of injury is limited access to diagnostic imaging. As part of a larger quality improvement initiative and to assist priority setting for policy makers, we evaluated trauma outcomes among patients who did and did not receive indicated imaging in the Emergency Department (ED). We hypothesize that receiving imaging is associated with increased early injury survival. We evaluated patterns of imaging performance in a prospective multi-site trauma registry cohort in Cameroon. All trauma patients enrolled in the Cameroon Trauma Registry (CTR) between 2017 and 2019 were included, regardless of injury severity. Patients prescribed diagnostic imaging were grouped into cohorts who did and did not receive their prescribed study. Patient demographics, clinical course, and outcomes were compared using chi-squared and Kruskal-Wallis tests. Multivariate logistic regression was used to explore associations between radiologic testing and survival after injury. Of 9,635 injured patients, 47.5% (4,574) were prescribed at least one imaging study. Of these, 77.8% (3,556) completed the study (COMPLETED) and 22.2% (1,018) did not receive the prescribed study (NC). Compared to COMPLETED patients, NC patients were younger (p = 0.02), male (p<0.01), and had markers of lower socioeconomic status (SES) (p<0.01). Multivariate regression adjusted for age, sex, SES, and injury severity demonstrated that receiving a prescribed study was strongly associated with ED survival (OR 5.00, 95% CI 3.32–7.55). Completing prescribed imaging was associated with increased early survival in injured Cameroonian patients. In a resource-limited setting, subsidizing access to diagnostic imaging may be a feasible target for improving trauma outcomes.
Optimizing the delivery of self-disseminating vaccines in fluctuating wildlife populations
Authors: Schreiner Courtney L. , Basinski Andrew J. , Remien Christopher H. , Nuismer Scott L.
Published: August 2023

Zoonotic pathogens spread by wildlife continue to spill into human populations and threaten human lives. A potential way to reduce this threat is by vaccinating wildlife species that harbor pathogens that are infectious to humans. Unfortunately, even in cases where vaccines can be distributed en masse as edible baits, achieving levels of vaccine coverage sufficient for pathogen elimination is rare. Developing vaccines that self-disseminate may help solve this problem by magnifying the impact of limited direct vaccination. Although models exist that quantify how well these self-disseminating vaccines will work when introduced into temporally stable wildlife populations, how well they will perform when introduced into populations with pronounced seasonal population dynamics remains unknown. Here we develop and analyze mathematical models of fluctuating wildlife populations that allow us to study how reservoir ecology, vaccine design, and vaccine delivery interact to influence vaccine coverage and opportunities for pathogen elimination. Our results demonstrate that the timing of vaccine delivery can make or break the success of vaccination programs. As a general rule, the effectiveness of self-disseminating vaccines is optimized by introducing after the peak of seasonal reproduction when the number of susceptible animals is near its maximum.
Orchids reduce attachment of herbivorous snails with leaf trichomes
Authors: Wati Richa Kusuma , Gravendeel Barbara , Langelaan Rob , van Heuven Bertie Joan , Claessens Jean , Kleynen Jacques , Smets Erik F. , de Winter Anton J. , van der Meijden Arie
Published: August 2023

Protective structures in the epidermis are essential for land plants to defend themselves against herbivores. In this study, we investigated the effect of different types of trichomes of three orchids, Calanthe triplicata, Dendrochilum pallidiflavens and Trichotosia ferox, on attachment of herbivorous land snails, using histochemistry and centrifuge experiments. Size, ornamentation and histochemistry of epicuticular trichomes on the orchid leaves were assessed with light microscopy, scanning electron microscopy and transmission electron microscopy. Total forces needed to detach two differently shaped snail species, Subulina octona and Pleurodonte isabella, were measured using a turntable equipped with a synchronized strobe. Snails were placed in two positions, either perpendicular or parallel to the main veins on the orchid leaves, both on the adaxial (= upper) or abaxial (= lower) side. The results obtained provided three new insights. First, a perpendicular or parallel position of the snails to the main veins did not significantly affect the attachment performance of either species tested. Secondly, snails detached significantly easier on leaf sides covered with a high density of lignin filled epicuticular trichomes. Thirdly, the removal of glandular trichomes did not affect the attachment forces; however, the absence of lignified trichomes increased the attachment of the snails. Our study highlights the importance of studying micro-ornamentation in combination with performance for obtaining a better understanding of the defense mechanisms employed by different species of orchids to deter herbivorous snails.
Exposure notification system activity as a leading indicator for SARS-COV-2 caseload forecasting
Authors: Aronoff-Spencer Eliah , Mazrouee Sepideh , Graham Rishi , Handcock Mark S. , Nguyen Kevin , Nebeker Camille , Malekinejad Mohsen , Longhurst Christopher A.
Published: August 2023

Purpose

Digital methods to augment traditional contact tracing approaches were developed and deployed globally during the COVID-19 pandemic. These “Exposure Notification (EN)” systems present new opportunities to support public health interventions. To date, there have been attempts to model the impact of such systems, yet no reports have explored the value of real-time system data for predictive epidemiological modeling.

Methods

We investigated the potential to short-term forecast COVID-19 caseloads using data from California’s implementation of the Google Apple Exposure Notification (GAEN) platform, branded as CA Notify. CA Notify is a digital public health intervention leveraging resident’s smartphones for anonymous EN. We extended a published statistical model that uses prior case counts to investigate the possibility of predicting short-term future case counts and then added EN activity to test for improved forecast performance. Additional predictive value was assessed by comparing the pandemic forecasting models with and without EN activity to the actual reported caseloads from 1–7 days in the future.

Results

Observation of time series presents noticeable evidence for temporal association of system activity and caseloads. Incorporating earlier ENs in our model improved prediction of the caseload counts. Using Bayesian inference, we found nonzero influence of EN terms with probability one. Furthermore, we found a reduction in both the mean absolute percentage error and the mean squared prediction error, the latter of at least 5% and up to 32% when using ENs over the model without.

Conclusions

This preliminary investigation suggests smartphone based ENs can significantly improve the accuracy of short-term forecasting. These predictive models can be readily deployed as local early warning systems to triage resources and interventions.
GWAS of adventitious root formation in roses identifies a putative phosphoinositide phosphatase (SAC9) for marker-assisted selection
Authors: Wamhoff David , Patzer Laurine , Schulz Dietmar Frank , Debener Thomas , Winkelmann Traud
Published: August 2023

Rose propagation by cuttings is limited by substantial genotypic differences in adventitious root formation. To identify possible genetic factors causing these differences and to develop a marker for marker-assisted selection for high rooting ability, we phenotyped 95 cut and 95 garden rose genotypes in a hydroponic rooting system over 6 weeks. Data on rooting percentage after 3 to 6 weeks, root number, and root fresh mass were highly variable among genotypes and used in association mappings performed on genotypic information from the WagRhSNP 68 K Axiom SNP array for roses. GWAS analyses revealed only one significantly associated SNP for rooting percentage after 3 weeks. Nevertheless, prominent genomic regions/peaks were observed and further analysed for rooting percentage after 6 weeks, root number and root fresh mass. Some of the SNPs in these peak regions were associated with large effects on adventitious root formation traits. Very prominent were ten SNPs, which were all located in a putative phosphoinositide phosphatase SAC9 on chromosome 2 and showed very high effects on rooting percentage after 6 weeks of more than 40% difference between nulliplex and quadruplex genotypes. SAC9 was reported to be involved in the regulation of endocytosis and in combination with other members of the SAC gene family to regulate the translocation of auxin-efflux PIN proteins via the dephosphorylation of phosphoinositides. For one SNP within SAC9, a KASP marker was successfully derived and used to select genotypes with a homozygous allele configuration. Phenotyping these homozygous genotypes for adventitious root formation verified the SNP allele dosage effect on rooting. Hence, the presented KASP derived from a SNP located in SAC9 can be used for marker-assisted selection in breeding programs for high rooting ability in the future.
Interaction effects between insomnia and depression on risk of out-of-hospital cardiac arrest: Multi-center study
Authors: Jung Eujene , Ryu Hyun Ho , Kim Sung Wan , Lee Jung Ho , Song Kyoung Jun , Ro Young Sun , Cha Kyoung Chul , Hwang Sung Oh ,   Phase II Cardiac Arrest Pursuit Trial with Unique Registry and Epidemiologic Surveillance (CAPTURES-II investigators)
Published: August 2023

Background

Insomnia and depression have been known to be risk factors of several diseases, including coronary heart disease. We hypothesized that insomnia affects the out-of-hospital cardiac arrest (OHCA) incidence, and these effects may vary depending on whether it is accompanied by depression. This study aimed to determine the association between insomnia and OHCA incidence and whether the effect of insomnia is influenced by depression.

Methods

This prospective multicenter case-control study was performed using Phase II Cardiac Arrest Pursuit Trial with Unique Registration and Epidemiology Surveillance (CAPTURES-II) project database for OHCA cases and community-based controls in Korea. The main exposure was history of insomnia. We conducted conditional logistic regression analysis to estimate the effect of insomnia on the risk of OHCA incidence and performed interaction analysis between insomnia and depression. Finally, subgroup analysis was conducted in the patients with insomnia.

Results

Insomnia was not associated with increased OHCA risk (0.95 [0.64–1.40]). In the interaction analysis, insomnia interacted with depression on OHCA incidence in the young population. Insomnia was associated with significantly higher odds of OHCA incidence (3.65 [1.29–10.33]) in patients with depression than in those without depression (0.84 [0.59–1.17]). In the subgroup analysis, depression increased OHCA incidence only in patients who were not taking insomnia medication (3.66 [1.15–11.66]).

Conclusion

Insomnia with depression is a risk factor for OHCA in the young population. This trend was maintained only in the population not consuming insomnia medication. Early and active medical intervention for patients with insomnia may contribute to lowering the risk of OHCA.
Intimate partner violence and its associated factors among reproductive-age women in East Africa:-A generalized mixed effect robust poisson regression model
Authors: Tessema Zemenu Tadesse , Gebrie Worku Misganaw , Tesema Getayeneh Antehunegn , Alemneh Tesfa Sewunet , Teshale Achamyeleh Birhanu , Yeshaw Yigizie , Alem Adugnaw Zeleke , Ayalew Hiwotie Getaneh , Liyew Alemneh Mekuriaw
Published: August 2023

Background

The World Health Organization (WHO) has published estimates revealing that around one out of every three women across the globe has been a victim of either physical and/or sexual violence from an intimate partner or non-partner throughout their lifetime. The available evidence on intimate partner violence in East Africa is limited Consequently, the objective of this study was to evaluate the occurrence and factors linked to intimate partner violence in East Africa.

Methods

The study utilized the most recent data from the Demographic and Health Surveys (DHS) conducted between 2011 and 2018/19 in 11 countries in Eastern Africa. A total of 59,000 women were included in the study. Descriptive and inferential statistics were used to exmine factors associated with IPV. A mixed effect robust Poisson regression model was fitted to identify factors associated with intimate partner violence. The adjusted prevalence ratio (aPR) and its corresponding 95% confidence interval (CI) were employed to determine the presence of a significant association between intimate partner violence and the independent variables.

Results

In this study, the prevalence of intimate partner violence in East Africa was 43.72% with 95% CI 43.32% to 44.12%. In the mixed effect robust Poisson regression model:—Marital status, working status, parity, sex of household headed, wealth index, community poverty, and residence, were significantly associated with intimate partner violence.

Conclusion

The prevalence of intimate partner violence in East Africa is high as compared to the global prevalence 30%, which hinders The Sustainable Development Goals (SDGs), specifically goal 5, aim to attain gender equality and empower women and girls worldwide by the year 2030 Women being previously married and cohabitated, working, having a high number of children, rural residents were positively associated with IPV and household and community wealth index and sex of household headed were negatively related with IPV in East Africa. Therefore, we recommend establishing effective health and legal response using an integrated policy approach and Special attention should be given to women who live rural and poorest to reduce IPV and to achieve Sustainable Development Goals (SDGs) goal 5.
Structural-demographic analysis of the Qing Dynasty (1644–1912) collapse in China
Authors: Orlandi Georg , Hoyer Daniel , Zhao Hongjun , Bennett James S. , Benam Majid , Kohn Kathryn , Turchin Peter
Published: August 2023

This paper analyzes the collapse of the Qing dynasty (1644–1912) through the lens of the Structural Demographic Theory (SDT), a general framework for understanding the drivers of socio-political instability in state-level societies. Although a number of competing ideas for the collapse have been proposed, none provide a comprehensive explanation that incorporates the interaction of all the multiple drivers involved. We argue that the four-fold population explosion peaking in the 19th century, the growing competition for a stagnant number of elite positions, and increasing state fiscal stress combined to produce an increasingly disgruntled populace and elite, leading to significant internal rebellions. We find that while neither the ecological disasters nor the foreign incursions during the 19th century were sufficient on their own to bring down the Qing, when coupled with the rising internal socio-political stresses, they produced a rapid succession of triggering events that culminated in the Qing collapse.
How researchers calculate students’ grade point average in other courses has minimal impact
Authors: Young Nicholas T. , Matz Rebecca L. , Bell Eric F. , Hayward Caitlin
Published: August 2023

Grade point average in “other” courses (GPAO) is an increasingly common measure used to control for prior academic performance and to predict future academic performance. In previous work, there are two distinct approaches to calculating GPAO, one based on only courses taken concurrently (term GPAO) and one based on all previous courses taken (cumulative GPAO). To our knowledge, no one has studied whether these methods for calculating the GPAO result in equivalent analyses and conclusions. As researchers often use one definition or the other without comment on why that choice was made, if the two calculations of GPAO are different, researchers might be inducing systematic error into their results and publishing potentially inaccurate conclusions. We looked at more than 3,700 courses at a public, research-intensive university over a decade and found limited evidence that the choice of GPAO calculation affects the conclusions. At most, one in seven courses could be affected. Further analysis suggests that there may be situations where one form of GPAO may be preferred over the other when it comes to examining inequity in courses or predicting student grades. However, we did not find sufficient evidence to universally recommend one form of GPAO over the other.
Strengthening systems to provide long-acting reversible contraceptives (LARCs) in public sector health facilities in Uganda and Zambia: Program results and learnings
Authors: Kamanga Aniset , Lyazi Micheal , Prust Margaret L. , Medina-Jaudes Naomi , Ngosa Lupenshyo , Nalwabwe Margaret , Ndhlovu Martha , Kaluba Dynes , Mwiche Angel , Mugahi Richard , Batusa Joy , Zulu Morrison , Musoke Andrew , Shakwele Hilda , Glover Caitlin , Aldrich Emma
Published: August 2023

Introduction

In Uganda and Zambia, both supply- and demand-side factors hamper availability of long-acting reversible contraceptives (LARCs), including implants and intrauterine devices (IUDs), at public sector facilities. This paper discusses results of a program aimed at increasing access to and uptake of LARC services in public sector facilities through capacity building of government health workers, strengthening government supply chains, and client mobilization.

Methods

From 2018–2021, the Ministries of Health (MOHs) in Uganda and Zambia and Clinton Health Access Initiative (CHAI) worked to increase readiness to provide LARC services within 51 focal facilities in Uganda and 85 focal facilities in Zambia. Annual facility assessments of LARC-related resources were conducted and routine service delivery data were monitored.

Results

At baseline, few focal facilities had supplies and skilled staff to provide LARC services. At endline, over 90% of focal facilities in both countries had a provider trained to provide both implants and IUDs and 55% had the commodities and equipment needed for implant provision. In Uganda and Zambia, respectively, 65% and 38% of focal facilities had commodities and equipment for IUD provision at endline. Both programs observed significant increases in the number of implants provided at focal facilities; in Uganda implant volumes increased five-fold from 4,560 at baseline to 23,463 at endline, and in Zambia implant volumes increased nearly four-fold from 1,884 at baseline to 7,394 at endline. Uganda did not observe growth in IUD volumes, whereas Zambia observed significantly increased IUD service volumes from 251 at baseline to 3,866 at endline.

Conclusions

Public sector facilities can be rapidly and sustainably capacitated to provide LARCs when both catalytic and systems strengthening interventions are deployed for health worker capacity building, supply chain management, and community mobilization to ensure client flow. Investments should be intentionally sequenced and coordinated to generate a virtuous cycle that enables continued LARC service provision.
Attitudes to the use of animals in biomedical research: Effects of stigma and selected research project summaries
Authors: Cassaday Helen J. , Cavenagh Lucy , Aluthgamage Hiruni , Crooks Aoife , Bonardi Charlotte , Stevenson Carl W. , Waite Lauren , Muir Charlotte
Published: August 2023

Three groups of participants (largely recruited from the UK) completed a survey to examine attitudes to the use of animals in biomedical research, after reading the lay (N = 182) or technical (N = 201) summary of a research project, or no summary (N = 215). They then completed a survey comprising the animal attitude (AAS), animal purpose (APQ), belief in animal mind (BAM) and empathy quotient (EQ) scales. The APQ was adapted to assess attitudes towards the use of animals for research into disorders selected to be perceived as controllable and so ‘blameworthy’ and potentially stigmatised (addiction and obesity) and ‘psychological’ (schizophrenia and addiction) versus ‘physical’ (cardiovascular disease and obesity), across selected species (rats, mice, fish pigs and monkeys). Thus, the APQ was used to examine how the effects of perceived controllability and the nature of the disorder affected attitudes to animal use, in different species and in the three summary groups. As expected, attitudes to animal use as measured by the AAS and the APQ (total) correlated positively with BAM and EQ scores, consistent with the assumption that the scales all measured pro-welfare attitudes. Participants in the two research summary groups did not differentiate the use of rats, mice and fish (or fish and pigs in the technical summary group), whereas all species were differentiated in the no summary group. Participants given the lay summary were as concerned about the use of animals for schizophrenia as for addiction research. APQ ratings otherwise indicated more concern for animals used for addiction research (and for obesity compared to cardiovascular disease in all summary groups). Therefore, the information provided by a research project summary influenced attitudes to use of animals in biomedical research. However, there was no overall increase in agreement with animal use in either of the summary groups.
The association between religious participation and memory among middle-aged and older adults: A systematic review
Authors: Nath Bonita , Patel Priya , Oremus Mark
Published: August 2023

Objectives

Mounting evidence suggests religion plays an important role in maintaining cognition. No prior systematic review has focused on the specific association between religion and the memory domain of cognition in middle-aged and older adults. We carried out a systematic review to explore this association in depth.

Methods

We searched the PsycINFO, Scopus, and PubMed databases to identify articles assessing any means of measuring religion as the exposure and memory as the outcome. Articles had to report on studies with comparison groups to be eligible for inclusion in the review. We followed the PRISMA checklist to conduct the review (PROSPERO registration # CRD42022330389).

Results

Nine out of the 1648 citations retrieved in the literature search were included in the review. The majority of included articles had a moderate risk of bias. Most results showed positive associations between religion and memory.

Discussion

Despite consistency in the direction of association between religion and memory, the literature contained some important research gaps: the studies were cross-sectional; a lack of information existed regarding whether different faiths, sex/gender and depression affected the association; and underpowered studies prevented us from drawing firm conclusions about the direction or magnitude of effect. Longitudinal studies avoiding these issues are needed in this field.
The effects of Eisenia fetida and Metaphire guillelmi on the soil micro-food web in a microcosm experiment
Authors: Niu Xinli , Cheng Yongfan , Feng Xiaopei , Zhao Wei , Zhang Xi , Du Mengjun , Gu Yanfang
Published: August 2023

Numerous studies have shown that the function of earthworms may depend on their ecotype and density, which affects how they impact soil microbial and nematode communities. However, it is unclear how different earthworm species and densities alter the composition of soil microbial and nematode communities and how these modifications impact the soil micro-food web. The structural equation model (SEM) is a more accurate tool for identifying the intricate relationships between various trophic levels in the soil micro-food webs than the widely used bivariate data analysis. In order to ascertain the effects of earthworm species, including epigeic earthworm Eisenia fetida and anecic earthworm Metaphire guillelmi, as well as varying densities on the composition of main microbial groups, soil nematodes and their relationships, a microcosm experiment was conducted in a greenhouse. After nine weeks of observation, compared with the control treatments, Eisenia fetida increased the biomasses of total microorganism and bacteria, whereas Metaphire guillelmi decreased the biomasses of total microorganism, bacteria, and gram-positive bacteria, but showed an increase in AMF biomass. Additionally, both two earthworm species decreased the abundance of total soil nematode, bacterivores, and omnivore-predators, which is in contrast with the control treatments. The SEM results indicated that the addition of Eisenia fetida at different densities had indirect negative effects on the abundance of omnivore-predators, as it significantly increased the content of soil organic carbon, ammonium nitrogen, and nitrate nitrogen. The bottom-up effects were found to be the dominant forces, which promoted bacterial-dominated decomposition channels. The addition of Metaphire guillelmi with different density had direct negative impact on bacterivores and fungivores. Moreover, it had indirect negative effects on omnivore-predators by altering the soil properties. The dominant forces were still the bottom-up effects. Our study suggests that different earthworm species have distinct mechanisms that affect the soil micro-food web in different ways.
Morphometric responses of two zooxanthellate octocorals along a water quality gradient in the Cuban northwestern coast
Authors: Rey-Villiers Néstor , Sánchez Alberto , González-Díaz Patricia , Álvarez-Filip Lorenzo
Published: August 2023

Octocoral abundance is increasing on Caribbean reefs, and one of the possible causes is their vertical morphological plasticity that allows them to grow above the substrate to reduce the effect of processes that occur in it (e.g., scour by sediments) as well as adapt to environmental gradients. The aim of this study was to determine the morphometric response of two octocorals species (Eunicea flexuosa and Plexaura kükenthali) with different life strategies in a water quality gradient. The research was carried out between 2008 and 2016 on eight forereefs of northwest Cuba. Different morphometric indicators were measured in the colonies of both species found within a belt transect (100 x 2 m) randomly located at each site. The lowest means in height, diameter, number of terminal branches/colony, cover index, and least arborescent colonies of E. flexuosa were detected at the sites with the greatest anthropogenic pollution. The water quality gradient did not explain the variability of the five morphometric indicators of P. kükenthali. However, hydrodynamic stress was the factor that most negatively affected the morphometry of this species. The chronic effect of poor water quality over time resulted in more small sized colonies of E. flexuosa at the polluted site, probably due to higher mortality. The size distribution of P. kükenthali also showed the same trend but at the sites with greater hydrodynamic stress. These results show that the morphometric response of octocorals along a water quality gradient is species-specific. This study suggests that poor water quality decreases the size and thus availability of habitat provided by octocorals sensitive to that factor (e.g., E. flexuosa) while other tolerant species (e.g., P. kükenthali) could provide the habitat of several organisms in a scenario of increasing anthropogenic pollution.
Reversal of pathological motor behavior in a model of Parkinson’s disease by striatal dopamine uncaging
Authors: Zamora-Ursulo Miguel A. , Perez-Becerra Job , Tellez Luis A. , Saderi Nadia , Carrillo-Reid Luis
Published: August 2023

Motor deficits observed in Parkinson’s disease (PD) are caused by the loss of dopaminergic neurons and the subsequent dopamine depletion in different brain areas. The most common therapy to treat motor symptoms for patients with this disorder is the systemic intake of L-DOPA that increases dopamine levels in all the brain, making it difficult to discern the main locus of dopaminergic action in the alleviation of motor control. Caged compounds are molecules with the ability to release neuromodulators locally in temporary controlled conditions using light. In the present study, we measured the turning behavior of unilateral dopamine-depleted mice before and after dopamine uncaging. The optical delivery of dopamine in the striatum of lesioned mice produced contralateral turning behavior that resembled, to a lesser extent, the contralateral turning behavior evoked by a systemic injection of apomorphine. Contralateral turning behavior induced by dopamine uncaging was temporarily tied to the transient elevation of dopamine concentration and was reversed when dopamine decreased to pathological levels. Remarkably, contralateral turning behavior was tuned by changing the power and frequency of light stimulation, opening the possibility to modulate dopamine fluctuations using different light stimulation protocols. Moreover, striatal dopamine uncaging recapitulated the motor effects of a low concentration of systemic L-DOPA, but with better temporal control of dopamine levels. Finally, dopamine uncaging reduced the pathological synchronization of striatal neuronal ensembles that characterize unilateral dopamine-depleted mice. We conclude that optical delivery of dopamine in the striatum resembles the motor effects induced by systemic injection of dopaminergic agonists in unilateral dopamine-depleted mice. Future experiments using this approach could help to elucidate the role of dopamine in different brain nuclei in normal and pathological conditions.
Enhancing anomaly detection in distributed power systems using autoencoder-based federated learning
Authors: Kea Kimleang , Han Youngsun , Kim Tae-Kyung
Published: August 2023

The growing use of Internet-of-Things devices in electric power systems has resulted in increased complexity and flexibility, making monitoring power usage critical for effective system maintenance and detecting abnormal behavior. However, traditional anomalous power consumption detection methods struggle to handle the vast amounts of data generated by these devices. While deep learning and machine learning are effective in anomaly detection, they require significant amounts of training data collected on centralized servers. This centralized approach results in high response time delays and data leakage problems. To address these challenges, we propose an Autoencoder-based Federated Learning method that combines the AutoEncoder and Federated Learning networks to develop a high-accuracy algorithm for identifying anomalies of power consumption data in distributed power systems. The proposed method allows for decentralized training of anomaly detection models among IoT devices, reducing response time and eventually solving data leakage issues. Our experimental results demonstrate the effectiveness of the FLAE method in detecting anomalies without needing data transferring.
DNAJB3 attenuates ER stress through direct interaction with AKT
Authors: Islam Zeyaul , Diane Abdoulaye , Khattab Namat , Dehbi Mohammed , Thornalley Paul , Kolatkar Prasanna R.
Published: August 2023

Metabolic stress involved in several dysregulation disorders such as type 2 diabetes mellitus (T2DM) results in down regulation of several heat shock proteins (HSPs) including DNAJB3. This down regulation of HSPs is associated with insulin resistance (IR) and interventions which induce the heat shock response (HSR) help to increase the insulin sensitivity. Metabolic stress leads to changes in signaling pathways through increased activation of both c-jun N-terminal kinase-1 (JNK1) and the inhibitor of κB inflammatory kinase (IKKβ) which in turn leads to inactivation of insulin receptor substrates 1 and 2 (IRS-1 and IRS-2). DNAJB3 interacts with both JNK1 and IKKβ kinases to mitigate metabolic stress. In addition DNAJB3 also activates the PI3K-PKB/AKT pathway through increased phosphorylation of AKT1 and its substrate AS160, a Rab GTPase-activating protein, which results in mobilization of GLUT4 transporter protein and improved glucose uptake. We show through pull down that AK T1 is an interacting partner of DNAJB3, further confirmed by isothermal titration calorimetry (ITC) which quantified the avidity of AKT1 for DNAJB3. The binding interface was identified by combining protein modelling with docking of the AKT1-DNAJB3 complex. DNAJB3 is localized in the cytoplasm and ER, where it interacts directly with AKT1 and mobilizes AS160 for glucose transport. Inhibition of AKT1 resulted in loss of GLUT4 translocation activity mediated by DNAJB3 and also abolished the protective effect of DNAJB3 on tunicamycin-induced ER stress. Taken together, our findings provide evidence for a direct protein-protein interaction between DNAJB3 and AKT1 upon which DNAJB3 alleviates ER stress and promotes GLUT4 translocation.
Associations between police harassment and distrust in and reduced access to healthcare among Black sexual minority men: A longitudinal analysis of HPTN 061
Authors: Feelemyer Jonathan P. , Duncan Dustin T. , Remch Molly , Kaufman Jay S. , Cleland Charles M. , Geller Amanda B. , Dyer Typhanye V. , Scheidell Joy D. , Turpin Rodman E. , Brewer Russell A. , Hucks-Ortiz Christopher , Mazumdar Medha , Mayer Kenneth H. , Khan Maria R.
Published: August 2023

Objective

Evaluate associations between racialized and homophobia-based police harassment (RHBPH) and healthcare distrust and utilization among Black Sexual Minority Men (BSMM).

Methods

We utilized data from a longitudinal cohort study from HIV Prevention Trials Network (HPTN) 061 with baseline, six and 12 month follow-up assessments. Using multivariable analysis, we evaluated associations between RHBPH and healthcare distrust and utilization reported at the 6 and 12 month visits.

Results

Of 1553 BSMM present at baseline, 1160 were available at six-month follow-up. In multivariable analysis, increasing frequency of RHBPH was associated with increasing levels of distrust in healthcare providers (aOR 1.31, 95% CI: 1.00, 1.74) and missing 50% or more of healthcare visits at six-month follow-up (aOR 1.93, 95% CI: 1.09, 3.43).

Conclusions

Recent experiences of RHBPH are associated with reduced trust in and access to healthcare among BSMM, with more frequent RHBPH associated with greater vulnerability.
Cognitive development among children in a low-income setting: Cost-effectiveness analysis of a maternal nutrition education intervention in rural Uganda
Authors: Ahmed Montasir , Muhoozi Grace K. M. , Atukunda Prudence , Westerberg Ane C. , Iversen Per O. , Wangen Knut R.
Published: August 2023

Inadequate nutrition and insufficient stimulation in early childhood can lead to long-term deficits in cognitive and social development. Evidence for policy and decision-making regarding the cost of delivering nutrition education is lacking in low and middle-income countries (LMIC). In rural Uganda, we conducted a cluster-randomized controlled trial (RCT) examining the effect of a maternal nutrition education intervention on developmental outcomes among children aged 6–8 months. This intervention led to significantly improved cognitive scores when the children reached the age of 20–24 months. When considering the potential for this intervention’s future implementation, the desired effects should be weighed against the increased costs. This study therefore aimed to assess the cost-effectiveness of this education intervention compared with current practice. Health outcome data were based on the RCT. Cost data were initially identified by reviewing publications from the RCT, while more detailed information was obtained by interviewing researchers involved in processing the intervention. This study considered a healthcare provider perspective for an 18-months’ time horizon. The control group was considered as the current practice for the future large-scale implementation of this intervention. A cost-effectiveness analysis was performed, including calculations of incremental cost-effectiveness ratios (ICERs). In addition, uncertainty in the results was characterized using one-way and probabilistic sensitivity analyses. The ICER for the education intervention compared with current practice was USD ($) 16.50 per cognitive composite score gained, with an incremental cost of $265.79 and an incremental cognitive composite score of 16.11. The sensitivity analyses indicated the robustness of these results. The ICER was sensitive to changes in cognitive composite score and the cost of personnel. The education intervention can be considered cost-effective compared with the current practice. The outcome of this study, including the cost analysis, health outcome, cost-effectiveness, and sensitivity analysis, can be useful to inform policymakers and stakeholders about effective resource allocation processes in Uganda and possibly other LMIC.
Repeatability of pupil diameter measurements using three different topography devices
Authors: Saad Amr , Steinberg Johannes , Frings Andreas
Published: August 2023

Purpose

To evaluate the intra- and inter-device repeatability of pupil diameter measurements using three different devices in patients prior to corneal refractive surgery.

Methods

We examined preoperative measurements from a total of 204 eyes (102 patients) scheduled for corneal refractive surgery at two private centers between July and December 2021. Three consecutive scans were performed with three different devices (Sirius anterior segment analyzer, Pentacam HR, IOLMaster 500) in the same session by the same examiner under standardized conditions. To assess the intra- and inter-device repeatability, we calculated the Intraclass Correlation Coefficient (ICC) and demonstrated results using Bland-Altman plots.

Results

The measurement accuracy (intra-device repeatability) of Sirius and IOLMaster was comparable (ICC = 0.64 and 0.61, respectively), with almost no statistically significant differences. Sirius showed the highest measurement accuracy among the three devices. Pentacam measurements resulted in lower precision, with an ICC of 0.09. The agreement between the pairs of devices (inter-device repeatability) was low (wide LoA ranges, Table 5).

Conclusion

In this study, the intra-device repeatability of Sirius and IOLMaster was higher than that of the Pentacam, although it did not achieve an optimal level across all three devices. The three devices examined cannot be used interchangeably.
Uncovering the phenotypic consequences of multi-locus imprinting disturbances using genome-wide methylation analysis in genomic imprinting disorders
Authors: Kim Hwa Young , Shin Choong Ho , Shin Chang Ho , Ko Jung Min
Published: August 2023

Imprinted genes are regulated by DNA methylation of imprinted differentially methylated regions (iDMRs). An increasing number of patients with congenital imprinting disorders (IDs) exhibit aberrant methylation at multiple imprinted loci, multi-locus imprinting disturbance (MLID). We examined MLID and its possible impact on clinical features in patients with IDs. Genome-wide DNA methylation analysis (GWMA) using blood leukocyte DNA was performed on 13 patients with Beckwith–Wiedemann syndrome (BWS), two patients with Silver–Russell syndrome (SRS), and four controls. HumanMethylation850 BeadChip analysis for 77 iDMRs (809 CpG sites) identified three patients with BWS and one patient with SRS showing additional hypomethylation, other than the disease-related iDMRs, suggestive of MLID. Two regions were aberrantly methylated in at least two patients with BWS showing MLID: PPIEL locus (chromosome 1: 39559298 to 39559744), and FAM50B locus (chromosome 6: 3849096 to 3849469). All patients with BWS- and SRS-MLID did not show any other clinical characteristics associated with additional involved iDMRs. Exome analysis in three patients with BWS who exhibited multiple hypomethylation did not identify any causative variant related to MLID. This study indicates that a genome-wide approach can unravel MLID in patients with an apparently isolated ID. Patients with MLID showed only clinical features related to the original IDs. Long-term follow-up studies in larger cohorts are warranted to evaluate any possible phenotypic consequences of other disturbed imprinted loci.