bluuebunny/crossref_metadata_2025 huggingface dataset:
OTIMIMALISASI FILING SYSTEM DOKUMEN REKAM MEDIS DI PUSKESMAS MADAPANGGA DAN BOLO TAHUN 2022
Authors: Syamsuriansyah
Published: June 2023

Semua fasilitas pelayanan kesehatan wajib menyelenggarakan rekam medis, termasuk puskesmas. Dengan pelayanan rekam medis yang berkualitas pasien akan merasa puas, khususnya karena pasien dilayani dengan cepat, tepat dan aman oleh pihak puskesmas. Permasalahan yang sering ditemui pada bagian penyimpanan rekam medis adalah terjadinya misfile maupun duplikasi nomor rekam medis. Akibatnya, berkas rekam medis pasien lama sulit ditemukan sehingga prosespencarian berkas rekam medis pasien di rak penyimpanan membutuhkan waktu yang cukup lama. Tujuan pelaksanaan Pengabdian Kepada Masyarakat (PKM) agar sistem penyimpanan rekam medis menjadi optimal dalam rangka peningkatan kualitas pelayanan rekam medis pasien rawat jalan di Puskesmas Madapangga. Metode yang digunakan berupa sosialisasi tentang optimalisasi sistem penyimpanan rekam medis di Puskesmas Madapangga dan Bolo. Sebelum sosialisasi, dilakukan pre-test dan setelah sosialisasi dilakukan kegiatan post-test dengan tujuan mengetahui tingkat pemahaman mitra tentang materi sosialisasi. PKM ini dilakukan pada tanggal 15 September 2022 di Puskesmas Madapangga. Peserta dari kegiatan PKM ini adalah semua petugas rekam medis di Puskesmas Madapangga. Madapangga sebanyak 6 (enam) orang dan Bolo sebanyak   orang. Hasil dari kegiatan PKM ini adalah adanya peningkatan pengetahuan mitra tentang penyimpanan rekam medis dari nilai rata-rata 45 menjadi 85 point.
PENYULUHAN PENTINGNYA MAKANAN JAJANAN SEHAT DAN BERGIZI PADA SISWA MTS Al-IKHLASIYAH PERAMPUAN
Authors: Asri Roushandy , Abdurahim
Published: June 2023

Makanan adalah salah satu kebutuhan dalam hidup manusia yang esensial bagi proses pertumbuhan dan perkembangan serta sebagai dasar pemeliharaan kesehatan, baik bagi anak maupun orang dewasa. Setiap makanan yang masuk ke dalam tubuh kita bisa mempengaruhi kerja metabolisme tubuh. Banyak ditemukan jajanan makanan yang diperjual belikan di sekitar sekolah mengandung zat tambahanan seperti pengawet dan pewarna makanan yang tidak seharusnya dipergunakan pada makanan. Kebiasaan jajan pada anak sangat erat dikaitkan dengan ekonomi dan tingkat pengetahuan anak maupun orang tua mengenai pemilihan jajanan makanan yang sehat dan bergizi. Kegiatan kali ini memberikan penyuluhan tentang Kesehatan Mengenai Pentingnya Makanan Jajanan Sehat dan Bergizi yang dapat diidentifikasikan dalam beberapa permasalahan spesifik, pengaruh Penyuluhan Kesehatan Mengenai Pentingnya Makanan Jajanan Sehat dan Bergizi. Pentingnya penyuluhan yaitu Meningkatkan pengetahuan siswa mengenai pentingnya jajanan sehat dan bergizi bagi kesehatan dan Mendorong menerapkan program jajanan sehat dan bergizi. Peserta penyuluhan adalah siswa MTS Al-Ikhlashiyah Perampuan sebanyak 20 orang.
Re-presentação em aplicativos de relacionamento
Authors: Toledo Jeanine , Lousa Teresa
Published: June 2023

O presente trabalho parte de um fundamento teórico abrangente para uma cartografia artística que tem em vista representar perfis masculinos em aplicativos de relacionamento. Essa cartografia, representa uma radical transformação de linguagem, que é objeto de estudo neste projeto. Esses perfis, por meio de uma operação ambígua, representam os selfies contemporâneos, muitas vezes através de personas/simulacros, os quais modificam a compreensão da autorrepresentação com vista ao relacionamento, configurando assim uma nova paisagem sentimental contemporânea. 

É o resultado da minha dissertação de Mestrado em Pintura na FBAUL, Faculdade de Belas Artes da Universidade de Lisboa, em 2022.
A holistic perspective on hearing loss: first quality-of-life questionnaire (HL-QOL) for people with hearing loss based on the international classification of functioning, disability, and health
Authors: Illg Angelika , Amann Edda , Koinig Karin A. , Anderson Ilona , Lenarz Thomas , Billinger-Finke Mareike
Published: June 2023

Introduction

To date, there is no quality-of-life questionnaire for people with hearing loss based on a multidisciplinary framework. Therefore, this study aimed to develop and validate a comprehensive assessment tool that addresses quality of life in people with hearing loss who use a cochlear implant based on the International Classification of Functioning, Disability, and Health (ICF).

Methods

In a first step, the Quality of Life in People with Hearing Loss Questionnaire (HL-QoL) was developed and tested for face validity. In a second step, the HL-QoL was evaluated and validated. In a third step, the HL-QoL was finalized based on the outcomes of the evaluation and validation.

Results

Eighty-four study participants fully completed the HL-QoL. The result of the test-retest reliability analysis was high and highly significant (n = 63; r = 0.914; p < 0.001). The mean total HL-QoL score (100.7 ± SD 24.58) suggests an overall high level of quality-of-life in this sample of people with hearing loss using a cochlear implant. The final version of the HL-QoL contains 21 items.

Conclusion

The HL-QoL has shown to be a valid and reliable tool to assess quality of life in people with hearing loss who use a cochlear implant. In addition to the total score, it is possible to calculate subscales based on the ICF components Body Functions and Activities and Participation.
Preliminary study on mesenchymal stem cells in repairing nerve injury in pelvic floor denervation
Authors: Zhang Guorui , Dai Yuxin , Lang Jinghe
Published: June 2023

Introduction: Nerve injury is considered one of the causes of pelvic floor dysfunction. Mesenchymal stem cells (MSCs) transplantation provides new possibilities for refractory degenerative diseases. This study aimed to explore the possibility and strategy of mesenchymal stem cells in treating pelvic floor dysfunction nerve injury.

Methods: MSCs were isolated from human adipose tissue and cultured. A MSCs suspension (40 µL at 5 × 107/mL) was loaded on a gelatin scaffold. A rat model of anterior vaginal wall nerve injury was established by bilateral pudendal nerve denervation. The nerve tissue repair effect of mesenchymal stem cells transplanted into the anterior vaginal wall of a rat model was explored and compared in the following three groups: blank gelatin scaffold group (GS group), mesenchymal stem cell injection group (MSC group), and mesenchymal stem cells loaded on the gelatin scaffold group (MSC-GS group). Nerve fiber counting under a microscope and mRNA expression of neural markers were tested. Moreover, mesenchymal stem cells were induced into neural stem cells in vitro, and their therapeutic effect was explored.

Results: Rat models of anterior vaginal wall nerve injury induced by bilateral pudendal nerve denervation showed a decreased number of nerve fibers in the anterior vaginal wall. qRT-PCR revealed that the content of neurons and nerve fibers in the rat model began to decrease 1 week after the operation and this could continue for 3 months. In vivo experiments showed that MSC transplantation improved the nerve content, and MSCs loaded on the gelatin scaffold had an even better effect. mRNA expression analysis demonstrated that MSCs loaded on gelatin scaffolds induced a higher and earlier gene expression of neuron-related markers. Induced neural stem cell transplantation was superior in improving the nerve content and upregulating the mRNA expression of neuron-related markers in the early stage.

Conclusion: MSCs transplantation showed a promising repair capacity for nerve damage in the pelvic floor. The supporting role of gelatin scaffolds might promote and strengthen the nerve repair ability at an early stage. Preinduction schemes could provide an improved regenerative medicine strategy for innervation recovery and functional restoration in pelvic floor disorders in the future.
Low-intensity pulsed ultrasound regulates osteoblast-osteoclast crosstalk via EphrinB2/EphB4 signaling for orthodontic alveolar bone remodeling
Authors: Zhou Jie , Zhu Yanlin , Ai Dongqing , Zhou Mengjiao , Li Han , Fu Yiru , Song Jinlin
Published: June 2023

Background: The limited regenerative potential of periodontal tissue remains a challenge in orthodontic treatment, especially with respect to alveolar bone remodeling. The dynamic balance between the bone formation of osteoblasts and the bone resorption of osteoclasts controls bone homeostasis. The osteogenic effect of low-intensity pulsed ultrasound (LIPUS) is widely accepted, so LIPUS is expected to be a promising method for alveolar bone regeneration. Osteogenesis is regulated by the acoustic mechanical effect of LIPUS, while the cellular perception, transduction mode and response regulation mechanism of LIPUS stimuli are still unclear. This study aimed to explore the effects of LIPUS on osteogenesis by osteoblast-osteoclast crosstalk and the underlying regulation mechanism.

Methods: The effects of LIPUS on orthodontic tooth movement (OTM) and alveolar bone remodeling were investigated via rat model by histomorphological analysis. Mouse bone marrow mesenchymal stem cells (BMSCs) and bone marrow monocytes (BMMs) were purified and used as BMSC-derived osteoblasts and BMM-derived osteoclasts, respectively. The osteoblast-osteoclast co-culture system was used to evaluate the effect of LIPUS on cell differentiation and intercellular crosstalk by Alkaline phosphatase (ALP), Alizarin Red S (ARS), tartrate-resistant acid phosphatase (TRAP) staining, real-time quantitative PCR, western blotting and immunofluorescence.

Results: LIPUS was found to improve OTM and alveolar bone remodeling in vivo, promote differentiation and EphB4 expression in BMSC-derived osteoblasts in vitro, particularly when cells were directly co-cultured with BMM-derived osteoclasts. LIPUS enhanced EphrinB2/EphB4 interaction between osteoblasts and osteoclasts in alveolar bone, activated the EphB4 receptor on osteoblasts membrane, transduced LIPUS-related mechanical signals to the intracellular cytoskeleton, and gave rise to the nuclear translocation of YAP in Hippo signaling pathway, thus regulating cell migration and osteogenic differentiation.

Conclusions: This study shows that LIPUS modulates bone homeostasis by osteoblast-osteoclast crosstalk via EphrinB2/EphB4 signaling, which benefits the balance between OTM and alveolar bone remodeling.
Lactate based caproate production with Clostridium drakei and process control of Acetobacterium woodii via lactate dependent in situ electrolysis
Authors: Herzog Jan , Mook Alexander , Utesch Tyll , Bengelsdorf Frank R. , Zeng An-Ping
Published: June 2023

Syngas fermentation processes with acetogens represent a promising process for the reduction of CO2 emissions alongside bulk chemical production. However, to fully realize this potential the thermodynamic limits of acetogens need to be considered when designing a fermentation process. An adjustable supply of H2 as electron donor plays a key role in autotrophic product formation. In this study an anaerobic laboratory scale continuously stirred tank reactor was equipped with an All-in-One electrode allowing for in-situ H2 generation via electrolysis. Furthermore, this system was coupled to online lactate measurements to control the co-culture of a recombinant lactate-producing Acetobacterium woodii strain and a lactate-consuming Clostridium drakei strain to produce caproate. When C. drakei was grown in batch cultivations with lactate as substrate, 1.6 g·L−1 caproate were produced. Furthermore, lactate production of the A. woodii mutant strain could manually be stopped and reinitiated by controlling the electrolysis. Applying this automated process control, lactate production of the A. woodii mutant strain could be halted to achieve a steady lactate concentration. In a co-culture experiment with the A. woodii mutant strain and the C. drakei strain, the automated process control was able to dynamically react to changing lactate concentrations and adjust H2 formation respectively. This study confirms the potential of C. drakei as medium chain fatty acid producer in a lactate-mediated, autotrophic co-cultivation with an engineered A. woodii strain. Moreover, the monitoring and control strategy presented in this study reinforces the case for autotrophically produced lactate as a transfer metabolite in defined co-cultivations for value-added chemical production.
Rapid and non-invasive diagnostic techniques for embryonic developmental potential: a metabolomic analysis based on Raman spectroscopy to identify the pregnancy outcomes of IVF-ET
Authors: Meng Hui , Huang Shan , Diao Feiyang , Gao Chao , Zhang Jun , Kong Lingyin , Gao Yan , Jiang Chunyan , Qin Lianju , Chen Ying , Xu Mengna , Gao Li , Liang Bo , Hu Yanqiu
Published: June 2023

The non-invasive and rapid assessment of the developmental potential of embryos is of great clinical importance in assisted reproductive technology (ART). In this retrospective study, we analyzed the metabolomics of 107 samples provided by volunteers and utilized Raman spectroscopy to detect the substance composition in the discarded culture medium of 53 embryos resulting in successful pregnancies and 54 embryos that did not result in pregnancy after implantation. The culture medium from D3 cleavage-stage embryos was collected after transplantation and a total of 535 (107 × 5) original Raman spectra were obtained. By combining several machine learning methods, we predicted the developmental potential of embryos, and the principal component analysis–convolutional neural network (PCA-CNN) model achieved an accuracy rate of 71.5%. Furthermore, the chemometric algorithm was used to analyze seven amino acid metabolites in the culture medium, and the data showed significant differences in tyrosine, tryptophan, and serine between the pregnancy and non-pregnancy groups. The results suggest that Raman spectroscopy, as a non-invasive and rapid molecular fingerprint detection technology, shows potential for clinical application in assisted reproduction.
An integrative systems biology strategy to support the development of adverse outcome pathways (AOPs): a case study on radiation-induced microcephaly
Authors: Jaylet Thomas , Quintens Roel , Armant Olivier , Audouze Karine
Published: June 2023

Adverse Outcome Pathways (AOPs) are useful tools for assessing the potential risks associated with exposure to various stressors, including chemicals and environmental contaminants. They provide a framework for understanding the causal relationships between different biological events that can lead to adverse outcomes (AO). However, developing an AOP is a challenging task, particularly in identifying the molecular initiating events (MIEs) and key events (KEs) that constitute it. Here, we propose a systems biology strategy that can assist in the development of AOPs by screening publicly available databases, literature with the text mining tool AOP-helpFinder, and pathway/network analyses. This approach is straightforward to use, requiring only the name of the stressor and adverse outcome to be studied. From this, it quickly identifies potential KEs and literature providing mechanistic information on the links between the KEs. The proposed approach was applied to the recently developed AOP 441 on radiation-induced microcephaly, resulting in the confirmation of the KEs that were already present and identification of new relevant KEs, thereby validating the strategy. In conclusion, our systems biology approach represents a valuable tool to simplify the development and enrichment of Adverse Outcome Pathways (AOPs), thus supporting alternative methods in toxicology.
Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity
Authors: Patsula Vitalii , Mareková Dana , Jendelová Pavla , Nahorniak Mykhailo , Shapoval Oleksandr , Matouš Petr , Oleksa Viktoriia , Konefał Rafał , Vosmanská Magda , Machová-Urdziková Lucia , Horák Daniel
Published: June 2023

Large (120 nm) hexagonal NaYF4:Yb, Er nanoparticles (UCNPs) were synthesized by high-temperature coprecipitation method and coated with poly(ethylene glycol)-alendronate (PEG-Ale), poly (N,N-dimethylacrylamide-co-2-aminoethylacrylamide)-alendronate (PDMA-Ale) or poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The colloidal stability of polymer-coated UCNPs in water, PBS and DMEM medium was investigated by dynamic light scattering; UCNP@PMVEMA particles showed the best stability in PBS. Dissolution of the particles in water, PBS, DMEM and artificial lysosomal fluid (ALF) determined by potentiometric measurements showed that all particles were relatively chemically stable in DMEM. The UCNP@Ale-PEG and UCNP@Ale-PDMA particles were the least soluble in water and ALF, while the UCNP@PMVEMA particles were the most chemically stable in PBS. Green fluorescence of FITC-Ale-modified UCNPs was observed inside the cells, demonstrating successful internalization of particles into cells. The highest uptake was observed for neat UCNPs, followed by UCNP@Ale-PDMA and UCNP@PMVEMA. Viability of C6 cells and rat mesenchymal stem cells (rMSCs) growing in the presence of UCNPs was monitored by Alamar Blue assay. Culturing with UCNPs for 24 h did not affect cell viability. Prolonged incubation with particles for 72 h reduced cell viability to 40%–85% depending on the type of coating and nanoparticle concentration. The greatest decrease in cell viability was observed in cells cultured with neat UCNPs and UCNP@PMVEMA particles. Thanks to high upconversion luminescence, high cellular uptake and low toxicity, PDMA-coated hexagonal UCNPs may find future applications in cancer therapy.
Deep ensemble learning and transfer learning methods for classification of senescent cells from nonlinear optical microscopy images
Authors: Sorrentino Salvatore , Manetti Francesco , Bresci Arianna , Vernuccio Federico , Ceconello Chiara , Ghislanzoni Silvia , Bongarzone Italia , Vanna Renzo , Cerullo Giulio , Polli Dario
Published: June 2023

The success of chemotherapy and radiotherapy anti-cancer treatments can result in tumor suppression or senescence induction. Senescence was previously considered a favorable therapeutic outcome, until recent advancements in oncology research evidenced senescence as one of the culprits of cancer recurrence. Its detection requires multiple assays, and nonlinear optical (NLO) microscopy provides a solution for fast, non-invasive, and label-free detection of therapy-induced senescent cells. Here, we develop several deep learning architectures to perform binary classification between senescent and proliferating human cancer cells using NLO microscopy images and we compare their performances. As a result of our work, we demonstrate that the most performing approach is the one based on an ensemble classifier, that uses seven different pre-trained classification networks, taken from literature, with the addition of fully connected layers on top of their architectures. This approach achieves a classification accuracy of over 90%, showing the possibility of building an automatic, unbiased senescent cells image classifier starting from multimodal NLO microscopy data. Our results open the way to a deeper investigation of senescence classification via deep learning techniques with a potential application in clinical diagnosis.
Anti-inflammatory effect of euphane- and tirucallane-type triterpenes isolated from the traditional herb Euphorbia neriifolia L
Authors: Chang Stephen S. , Huang Hung-Tse , Wei Wen-Chi , Lo I-Wen , Lin Yu-Chi , Chao Chih-Hua , Liao Geng-You , Shen Yuh-Chiang , Chen Jih-Jung , Li Tsung-Lin , Lin Liang-Tzung , Tai Chen-Jei , Kuo Yao-Haur , Liaw Chia-Ching
Published: June 2023

The Euphorbiaceae plant Euphorbia neriifolia L. is distributed widely in India, Thailand, Southeastern China, and Taiwan and used as a carminative and expectorant to treat several inflammation-related diseases, such as gonorrhoea, asthma, and cancer. In the course of our search for potential anti-inflammatory agents from the titled plant, 11 triterpenes from the stem of E. neriifolia were isolated and reported in our previous endeavor. Given its rich abundance in triterpenoids, the ethanolic extract in this follow-up exploration has led to the isolation of additional eight triterpenes, including six new euphanes—neritriterpenols H and J–N (1 and 3–7)—one new tirucallane, neritriterpenol I (2), and a known compound, 11-oxo-kansenonol (8). Their chemical structures were elucidated on the basis of spectroscopic data, including 1D- and 2D NMR, and HRESIMS spectra. The absolute stereochemistry of neritriterpenols was determined by single-crystal X-ray diffraction analysis, ICD spectra, and DP4+ NMR data calculations. Compounds 1–8 were also evaluated for their anti-inflammatory activity by using lipopolysaccharide (LPS)-stimulated IL-6 and TNF-α on RAW 264.7 macrophage cells. Intriguingly, the euphane-type triterpenes (1 and 3–8) showed an inhibitory effect on LPS-induced IL-6 but not on TNF-α, while tirucallane-type triterpene 2 showed strong inhibition on both IL-6 and TNF-α.
Metabolic pathway prediction of core microbiome based on enterotype and orotype
Authors: Lee Song Hee , Lee Han , You Hee Sang , Sung Ho-joong , Hyun Sung Hee
Published: June 2023

Introduction

Identification of key microbiome components has been suggested to help address the maintenance of oral and intestinal health in humans. The core microbiome is similar in all individuals, whereas the diverse microbiome varies across individuals, based on their unique lifestyles and phenotypic and genotypic determinants. In this study, we aimed to predict the metabolism of core microorganisms in the gut and oral environment based on enterotyping and orotyping.

Materials and methods

Gut and oral samples were collected from 83 Korean women aged 50 years or older. The extracted DNA was subjected to next-generation sequencing analysis of 16S rRNA hypervariable regions V3–V4.

Results

Gut bacteria were clustered into three enterotypes, while oral bacteria were clustered into three orotypes. Sixty-three of the core microbiome between the gut and oral population were correlated, and different metabolic pathways were predicted for each type. Eubacterium_g11, Actinomyces, Atopobium, and Enterococcus were significantly positively correlated between the gut and oral abundance. The four bacteria were classified as type 3 in orotype and type 2 in enterotype.

Conclusion

Overall, the study suggested that collapsing the human body’s multidimensional microbiome into a few categories may help characterize the microbiomes better and address health issues more deeply.
Circular Health: exploiting the SDG roadmap to fight AMR
Authors: Mantegazza Luca , De Pascali Alessandra Mistral , Munoz Olga , Manes Costanza , Scagliarini Alessandra , Capua Ilaria
Published: June 2023

Circular Health is a novel approach to address complex health issues that is based on the expansion of the One Health Paradigm. Circular health recognizes the need for a multidisciplinary convergence effort to complement the biomedical dimension of health. Antimicrobial resistance (AMR) is one of the greatest global concerns for public health that is likely on the rise, given the extensive use of antibiotics during the early Covid-19 years. Prior to the Covid-19 pandemic, an expert group chaired by Jim O’Neill published “The Review on Antimicrobial Resistance”, which contains a final report and recommendations on how to tackle AMR. The report, for the first time, considers AMR from a multi-perspective viewpoint highlighting how it cannot be successfully addressed unless there is a converging approach encompassing many dimensions of the problem. In this perspective, we propose to include the recommendations from that seminal report and other more recent reviews which include the lessons learnt from the Covid-19 pandemic, into the operational framework of the sustainable development goals (SDGs). AMR represents a perfect case study to explore how the SDG roadmap has the potential of becoming the driving force and implementation tool to address complex health issues by pursuing the optimization of resources and actions via a convergent and multi-stakeholder approach. The implementation of health-related policies through the whole spectrum of the SDGs could be both a novel and a well-established framework to inform multi-dimensional policies for more sustainable health in the future.
Differential cellular and humoral immune responses in immunocompromised individuals following multiple SARS-CoV-2 vaccinations
Authors: Meredith Rhys T. , Bermingham Max D. , Bentley Kirsten , Agah Sayeh , Aboagye-Odei Abigail , Yarham Ross A. R. , Mills Hayley , Shaikh Muddassir , Hoye Neil , Stanton Richard J. , Chadwick David R. , Oliver Maria A.
Published: June 2023

Introduction

The heterogeneity of the immunocompromised population means some individuals may exhibit variable, weak or reduced vaccine-induced immune responses, leaving them poorly protected from COVID-19 disease despite receiving multiple SARS-CoV-2 vaccinations. There is conflicting data on the immunogenicity elicited by multiple vaccinations in immunocompromised groups. The aim of this study was to measure both humoral and cellular vaccine-induced immunity in several immunocompromised cohorts and to compare them to immunocompetent controls.

Methods

Cytokine release in peptide-stimulated whole blood, and neutralising antibody and baseline SARS-CoV-2 spike-specific IgG levels in plasma were measured in rheumatology patients (n=29), renal transplant recipients (n=46), people living with HIV (PLWH) (n=27) and immunocompetent participants (n=64) post third or fourth vaccination from just one blood sample. Cytokines were measured by ELISA and multiplex array. Neutralising antibody levels in plasma were determined by a 50% neutralising antibody titre assay and SARS-CoV-2 spike specific IgG levels were quantified by ELISA.

Results

In infection negative donors, IFN-γ, IL-2 and neutralising antibody levels were significantly reduced in rheumatology patients (p=0.0014, p=0.0415, p=0.0319, respectively) and renal transplant recipients (p<0.0001, p=0.0005, p<0.0001, respectively) compared to immunocompetent controls, with IgG antibody responses similarly affected. Conversely, cellular and humoral immune responses were not impaired in PLWH, or between individuals from all groups with previous SARS-CoV-2 infections.

Discussion

These results suggest that specific subgroups within immunocompromised cohorts could benefit from distinct, personalised immunisation or treatment strategies. Identification of vaccine non-responders could be critical to protect those most at risk.
Detection of honey bee viruses in larvae of Vespa orientalis
Authors: Power Karen , Martano Manuela , Ragusa Ernesto , Altamura Gennaro , Maiolino Paola
Published: June 2023

The Oriental hornet (Vespa orientalis) is one of the major predators of honey bees. It has been demonstrated that adults of V. orientalis can harbor honey bee viruses, however the transmission route of infection is still not clear. The aim of this study was to study the possible presence of honey bee viruses in V. orientalis larvae and honey bees collected from the same apiary. Therefore, 29 samples of V. orientalis larvae and 2 pools of honey bee (Apis mellifera). samples were analyzed by multiplex PCR to detect the presence of six honeybee viruses: Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Kashmir Bee Virus (KBV) and Sac Brood Virus (SBV). Biomolecular analysis of V. orientalis larvae revealed that DWV was present in 24/29 samples, SBV in 10/29, BQCV in 7/29 samples and ABPV in 5/29 samples, while no sample was found positive for CBPV or KBV. From biomolecular analysis of honey bee samples DWV was the most detected virus, followed by SBV, BQCV, ABPV. No honey bee sample was found positive for CBPV or KBV. Considering the overlapping of positivities between V.orientalis larvae and honey bee samples, and that V.orientalis larvae are fed insect proteins, preferably honey bees, we can suggest the acquisition of viral particles through the ingestion of infected bees. However, future studies are needed to confirm this hypothesis and rule out any other source of infection.
Recent applications and potential of near-term (interannual to decadal) climate predictions
Authors: O'Kane Terence J. , Scaife Adam A. , Kushnir Yochanan , Brookshaw Anca , Buontempo Carlo , Carlin David , Connell Richenda K. , Doblas-Reyes Francisco , Dunstone Nick , Förster Kristian , Graça Antonio , Hobday Alistair J. , Kitsios Vassili , van der Laan Larissa , Lockwood Julia , Merryfield William J. , Paxian Andreas , Payne Mark R. , Reader M. Catherine , Saville Geoffrey R. , Smith Doug , Solaraju-Murali Balakrishnan , Caltabiano Nico , Carman Jessie , Hawkins Ed , Keenlyside Noel , Kumar Arun , Matei Daniela , Pohlmann Holger , Power Scott , Raphael Marilyn , Sparrow Michael , Wu Bo
Published: June 2023

Following efforts from leading centres for climate forecasting, sustained routine operational near-term climate predictions (NTCP) are now produced that bridge the gap between seasonal forecasts and climate change projections offering the prospect of seamless climate services. Though NTCP is a new area of climate science and active research is taking place to increase understanding of the processes and mechanisms required to produce skillful predictions, this significant technical achievement combines advances in initialisation with ensemble prediction of future climate up to a decade ahead. With a growing NTCP database, the predictability of the evolving externally-forced and internally-generated components of the climate system can now be quantified. Decision-makers in key sectors of the economy can now begin to assess the utility of these products for informing climate risk and for planning adaptation and resilience strategies up to a decade into the future. Here, case studies are presented from finance and economics, water management, agriculture and fisheries management demonstrating the emerging utility and potential of operational NTCP to inform strategic planning across a broad range of applications in key sectors of the global economy.
Quantifying attention span across the lifespan
Authors: Simon Alexander J. , Gallen Courtney L. , Ziegler David A. , Mishra Jyoti , Marco Elysa J. , Anguera Joaquin A. , Gazzaley Adam
Published: June 2023

Introduction

Studies examining sustained attention abilities typically utilize metrics that quantify performance on vigilance tasks, such as response time and response time variability. However, approaches that assess the duration that an individual can maintain their attention over time are lacking.

Methods

Here we developed an objective attention span metric that quantified the maximum amount of time that a participant continuously maintained an optimal “in the zone” sustained attention state while performing a continuous performance task.

Results

In a population of 262 individuals aged 7–85, we showed that attention span was longer in young adults than in children and older adults. Furthermore, declines in attention span over time during task engagement were related to clinical symptoms of inattention in children.

Discussion

These results suggest that quantifying attention span is a unique and meaningful method of assessing sustained attention across the lifespan and in populations with inattention symptoms.
Deep learning and gradient-based extraction of bug report features related to bug fixing time
Authors: Noyori Yuki , Washizaki Hironori , Fukazawa Yoshiaki , Ooshima Keishi , Kanuka Hideyuki , Nojiri Shuhei
Published: June 2023

Bug reports typically contain detailed descriptions of failures, hints at the location of the corresponding defects, and discussions. Developers usually resolve bugs using comments in descriptions and discussions. The time to fix a bug varies greatly. Previous studies have investigated bug reports, but the influence of comments on bug fixing time is not well understood. This study adopts a convolutional neural network (CNN) and gradient-based visualization approach called Grad-cam to elucidate the impact of comments on bug fixing time and extract features. A feature represents an observed characteristic in a bug report when processing via deep learning. Specifically, CNN classifies bug reports, and then Grad-cam visualizes the decision basis of CNN by identifying the top 10 word sequences used in the prediction. Here, the features are major word sequences extracted by Grad-cam. In an experiment, the proposed method classified more than 36,000 actual bug reports from Bugzilla with an accuracy of 75%–80%. Additionally, the visualization highlighted differences in the stack trace and word abstraction by bug fixing time. Bug reports with short bug fixing times are concrete, whereas those with a long bug fixing time are abstract.
Plasma homocysteine levels and associated factors in community-dwelling adolescents: the EVA-TYROL study
Authors: Gande Nina , Hochmayr Christoph , Staudt Anna , Bernar Benoît , Stock Katharina , Kiechl Sophia J. , Geiger Ralf , Griesmacher Andrea , Scholl-Bürgi Sabine , Knoflach Michael , Pechlaner Raimund , Kiechl-Kohlendorfer Ursula ,   the (Early Vascular Ageing) EVA Study Group
Published: June 2023

Background

Homocysteine (Hcy) has been associated with an adverse cardiovascular risk profile in adolescents. Assessment of the association between plasma Hcy levels and clinical/laboratory factors might improve our understanding of the pathogenesis of cardiovascular disease.

Methods

Hcy was measured in 1,900 14- to 19-year-old participants of prospective population-based EVA-TYROL Study (44.3% males, mean age 16.4 years) between 2015 and 2018. Factors associated with Hcy were assessed by physical examination, standardized interviews, and fasting blood analysis.

Results

Mean plasma Hcy was 11.3 ± 4.5 µmol/L. Distribution of Hcy was characterized by extreme right skew. Males exhibited higher Hcy and sex differences increased with increasing age. Univariate associations with Hcy emerged for age, sex, body mass index, high-density lipoprotein cholesterol, and for factors pertaining to blood pressure, glucose metabolism, renal function, and diet quality, whereas the most important multivariate predictors of Hcy were sex and creatinine.

Discussion

Clinical and laboratory factors associated with Hcy in adolescents were manifold, with sex and high creatinine identified as strongest independent determinants. These results may aid when interpreting future studies investigating the vascular risk of homocysteine.
Cardiac T1 mapping enables risk prediction of LV dysfunction after surgery for aortic regurgitation
Authors: Sinn Martin , Petersen Johannes , Lenz Alexander , von Stumm Maria , Sequeira Groß Tatiana Maria , Huber Lukas , Reichenspurner Hermann , Adam Gerhard , Lund Gunnar , Bannas Peter , Girdauskas Evaldas
Published: June 2023

Background

To assess whether cardiac T1 mapping for detecting myocardial fibrosis enables preoperative identification of patients at risk for early left ventricular dysfunction after surgery of aortic regurgitation.

Methods

1.5 Tesla cardiac magnetic resonance imaging was performed in 40 consecutive aortic regurgitation patients before aortic valve surgery. Native and post-contrast T1 mapping was performed using a modified Look-Locker inversion-recovery sequence. Serial echocardiography was performed at baseline and 8 ± 5 days after aortic valve surgery to quantify LV dysfunction. Receiver operating characteristic analysis was performed to determine the diagnostic accuracy of native T1 mapping and extracellular volume for predicting postoperative LV ejection fraction decrease >−10% after aortic valve surgery.

Results

Native T1 was significantly increased in patients with a postoperatively decreased LVEF (n = 15) vs. patients with a preserved postoperative LV ejection fraction (n = 25) (i.e., 1,071 ± 67 ms vs. 1,019 ± 33 ms, p = .001). Extracellular volume was not significantly different between patients with preserved vs. decreased postoperative LV ejection fraction. With a cutoff-of value of 1,053 ms, native T1 yielded an area under the curve (AUC) of .820 (95% CI: .683–.958) for differentiating between patients with preserved vs. reduced LV ejection fraction with 70% sensitivity and 84% specificity.

Conclusion

Increased preoperative native T1 is associated with a significantly higher risk of systolic LV dysfunction early after aortic valve surgery in aortic regurgitation patients. Native T1 could be a promising tool to optimize the timing of aortic valve surgery in patients with aortic regurgitation to prevent early postoperative LV dysfunction.
Determination of lethal electric field threshold for pulsed field ablation in ex vivo perfused porcine and human hearts
Authors: Kos Bor , Mattison Lars , Ramirez David , Cindrič Helena , Sigg Daniel C. , Iaizzo Paul A. , Stewart Mark T. , Miklavčič Damijan
Published: June 2023

Introduction

Pulsed field ablation is an emerging modality for catheter-based cardiac ablation. The main mechanism of action is irreversible electroporation (IRE), a threshold-based phenomenon in which cells die after exposure to intense pulsed electric fields. Lethal electric field threshold for IRE is a tissue property that determines treatment feasibility and enables the development of new devices and therapeutic applications, but it is greatly dependent on the number of pulses and their duration.

Methods

In the study, lesions were generated by applying IRE in porcine and human left ventricles using a pair of parallel needle electrodes at different voltages (500–1500 V) and two different pulse waveforms: a proprietary biphasic waveform (Medtronic) and monophasic 48 × 100 μs pulses. The lethal electric field threshold, anisotropy ratio, and conductivity increase by electroporation were determined by numerical modeling, comparing the model outputs with segmented lesion images.

Results

The median threshold was 535 V/cm in porcine ((N = 51 lesions in n = 6 hearts) and 416 V/cm in the human donor hearts ((N = 21 lesions in n = 3 hearts) for the biphasic waveform. The median threshold value was 368 V/cm in porcine hearts ((N = 35 lesions in n = 9 hearts) cm for 48 × 100 μs pulses.

Discussion

The values obtained are compared with an extensive literature review of published lethal electric field thresholds in other tissues and were found to be lower than most other tissues, except for skeletal muscle. These findings, albeit preliminary, from a limited number of hearts suggest that treatments in humans with parameters optimized in pigs should result in equal or greater lesions.
Identification of two novel ferroptosis-associated targets in sepsis-induced cardiac injury: Hmox1 and Slc7a11
Authors: Xu Yushun , Bu Gang
Published: June 2023

Purpose

Sepsis-induced cardiac injury is a severe complication of sepsis and has a high mortality. Recent research has implicated ferroptosis as a contributing factor to myocardial cell death. This study is aimed at finding novel ferroptosis-associated targets in sepsis-induced cardiac injury.

Methods and results

In our study, a total of two Gene expression omnibus datasets (GSE185754 and GSE171546) were obtained for bioinformatics analysis. GSEA enrichment analysis demonstrated that ferroptosis pathway Z-score rapidly increased in the first 24 h and decreased gradually in the following 24–72 h. Fuzzy analysis was then used to obtain distinct clusters of temporal patterns and find genes in cluster 4 that exhibited the same trend with ferroptosis progression during the time points. After intersecting the differentially expressed genes, genes in cluster 4, and ferroptosis-related genes, three ferroptosis-associated targets were finally selected: Ptgs2, Hmox1, and Slc7a11. While Ptgs2 has been previously reported to be involved in the regulation of septic cardiomyopathy, this study is the first to demonstrate that downregulation of Hmox1 and Slc7a11 can alleviate ferroptosis in sepsis-induced cardiac injury.

Conclusion

This study reports Hmox1 and Slc7a11 as ferroptosis-associated targets in sepsis-induced cardiac injury, and both of them may become key therapeutic and diagnostic targets for this complication in the future.
A case report and literature review: pheochromocytoma-mediated takotsubo cardiomyopathy, which is similar to acute myocardial infarction
Authors: Zhang Zhiyu , Guo Xia , Wang Jingyue , Wang Shipeng , Wang Yushi
Published: June 2023

A 52-year-old Chinese woman was admitted to a cardiac intensive care unit (CCU) due to nausea, vomiting, and dyspnea, which began a day before her hospitalization. Metoprolol succinate and conventional treatment for acute myocardial infarction (AMI) were initially administered to the patient based on electrocardiogram (ECG) findings and elevated cardiac troponin I (cTnI). However, the following day, she developed aggravated nausea, vomiting, fever, sweating, a flushed face, a rapid heart rate, and a significant rise in blood pressure. Furthermore, ultrasonic cardiography (UCG) displayed takotsubo-like changes; nevertheless, ECG indicated inconsistent cTnI peaks with extensive infarction. After coronary computed tomography angiography (CTA) ruled out (AMI), and in conjunction with the uncommon findings, we strongly suspected that the patient had a secondary condition of pheochromocytoma-induced takotsubo cardiomyopathy (Pheo-TCM). In the meanwhile, the use of metoprolol succinate was promptly discontinued. This hypothesis was further supported by the subsequent plasma elevation of multiple catecholamines and contrast-enhanced computed tomography (CECT). After one month of treatment with high-dose Phenoxybenzamine in combination with metoprolol succinate, the patient met the criteria for surgical excision and successfully underwent the procedure. This case report demonstrated that pheochromocytoma could induce TCM and emphasized the significance of distinguishing it from AMI (in the context of beta-blocker usage and anticoagulant management).
Tracking timescales of magma reservoir recharge through caldera cycles at Santorini (Greece). Emphasis on an explosive eruption of Kameni Volcano
Authors: Polo-Sánchez Antonio , Flaherty Taya , Hervé Garance , Druitt Tim , Fabbro Gareth N. , Nomikou Paraskevi , Balcone-Boissard Helène
Published: June 2023

Pre-eruptive processes and their timescales are critical information for risk management at explosive volcanoes, and Santorini caldera (Greece) provides an excellent context in which to approach this subject. We ask two questions. First, are pre-eruptive processes the same for small and big eruptions? To investigate, we performed a multi-mineral diffusion timescale study of a small explosive eruption of Kameni Volcano and compared the results with those published for larger caldera-forming eruptions at Santorini. The Kameni dacite resembles products of larger eruptions in being crystal-poor, containing plagioclase with antecrystic cores and autocrystic rims, bearing orthopyroxene with sector zoning and phantom skeletal morphologies, and showing evidence for mixing of different silicic magmas prior to eruption. Diffusion timescales from Mg-Fe profiles in orthopyroxene and clinopyroxene phenocrysts are <1–23 years, and Mg diffusion modelling in plagioclase gives <10 years. Our physical model for the Kameni eruption is similar to those proposed for larger eruptions, where silicic melt produced in gabbroic to dioritic lower to middle crustal mush bodies is transferred (along with entrained mafic magma) to an upper crustal reservoir. Crystals grow in the hydrous silicic melts due to decompression, cooling, and magma mixing during ascent and injection into upper crust. We propose that large eruptions are preceded by similar processes as small ones, but on a larger scale. Our second question: do diffusion timescales relate to eruptive volume or position in a caldera cycle? For this, we obtained orthopyroxene Mg-Fe diffusion timescales for three additional eruptions, growing our orthopyroxene timescale database to seven eruptions of different sizes and cycle timings. No clear relationship exists between diffusion timescale and volume; however, timescales are systematically shorter (<0.01–10 years) early in a cycle and longer (1–5,000 years) late in a cycle. Thermal maturation and H2O-flushing of the crustal magma reservoir through the caldera cycle could explain this, as the reservoir would change from a rigid to more mushy state as the cycle progresses. This would change the mechanical response to melt input and allow accumulation of progressively larger melt layers in the upper crust, resulting in increasing crystal residence times.
Main controlling factors of natural fractures in tight reservoirs of the lucaogou formation in the jimsar sag, Xinjiang, China
Authors: Yu Yixin , Deng Ze , Gao Jinliang , Li Peng , Zhang Chen
Published: June 2023

Natural fractures act as critical flow channels and reservoir space in the Lucaogou Formation tight reservoir of the Jimsar Sag. It is essential to identify the main controlling factors of natural fractures in order to achieve efficient development of tight oil in this area. There are mainly three types of natural fractures, including tectonic fractures, diagenetic fractures, and abnormal overpressure-induced fractures. Diagenetic fractures are predominantly bedding seams. The fracture development is affected by multiple factors including brittle minerals, lithology, tectonic stress, bed thickness, and total organic carbon (TOC). Large tectonic stress, smaller bed thickness, and higher total organic carbon are all favorable for the development of tectonic fractures and bedding seams. The controls of brittle minerals and lithology on fracture development are different for tectonic fractures and bedding seams. Specifically, carbonate minerals stimulate the tectonic fracture development, while brittle minerals have no control over the bedding seam development; tectonic fractures are most developed in the dolomitic rocks, while bedding seams are most developed in the argillaceous rocks. The calculated fracture density variation coefficients reveal that tectonic stress and brittle minerals are the main controlling factors of tectonic fracture development; total organic carbon and lithology are the main control factors of bedding seam development.
GIS-based landslide susceptibility modeling using data mining techniques
Authors: Xia Liheng , Shen Jianglong , Zhang Tingyu , Dang Guangpu , Wang Tao
Published: June 2023

Introduction: Landslide is one of the most widespread geohazards around the world. Therefore, it is necessary and meaningful to map regional landslide susceptibility for landslide mitigation. In this research, landslide susceptibility maps were produced by four models, namely, certainty factors (CF), naive Bayes (NB), J48 decision tree (J48), and multilayer perceptron (MLP) models.

Methods: In the first step, 328 landslides were identified via historical data, interpretation of remote sensing images, and field investigation, and they were divided into two subsets that were assigned different uses: 70% subset for training and 30% subset for validating. Then, twelve conditioning factors were employed, namely, altitude, slope angle, slope aspect, plan curvature, profile curvature, TWI, NDVI, distance to rivers, distance to roads, land use, soil, and lithology. Later, the importance of each conditioning factor was analyzed by average merit (AM) values, and the relationship between landslide occurrence and various factors was evaluated using the certainty factor (CF) approach. In the next step, the landslide susceptibility maps were produced based on four models, and the effect of the four models were quantitatively compared by receiver operating characteristic (ROC) curves, area under curve (AUC) values, and non-parametric tests.

Results: The results demonstrated that all the four models can reasonably assess landslide susceptibility. Of these four models, the CF model has the best predictive performance for the training (AUC=0.901) and validating data (AUC=0.892).

Discussion: The proposed approach is an innovative method that may also help other scientists to develop landslide susceptibility maps in other areas and that could be used for geo-environmental problems besides natural hazard assessments.