bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Spatio-Temporal Variations in Water and Land Resources and Their Driving Mechanism Based on the Coupling Coordination Model: A Case Study in Western Jilin Province, China
Authors: Zhang Lujuan , Aihemaitijiang Guzailinuer , Wan Zihao , Li Mingtang , Zhang Jiquan , Zhang Feng , Zhao Chunli
Published: January 2025

Water and land resources (WLR) are the most important basic resources for social and economic development. The effective alignment of WLR is crucial for maximizing resource utilization and promoting sustainable regional development. This study focuses on Western Jilin Province (WJP), China, employing the degree of coupling coordination model, spatial autocorrelation, and the center of gravity transfer model to assess and characterize the spatio-temporal differentiation patterns of water and land resource matching from 2006 to 2020. Five indicators—annual average temperature (AAT), urbanization rate (UR), population density (PD), reclamation rate (RR), and water resource utilization rate (WRUR)—were selected as influencing factors. A Tobit model was constructed to elucidate the driving mechanisms behind the evolution of the WLR coupling coordination degree (CCD) in WJP. The results indicate the following: (1) From a temporal perspective, the coupling coordination degree of WLR in WJP has shown a year-on-year increase from 2006 to 2020, transitioning from a moderate imbalance to intermediate coordination, reflecting a trend of continuous improvement. (2) Regarding spatial distribution, the overall center of gravity of water and land resource coupling coordination remained relatively stable between 2006 and 2020; however, the direction of distribution gradually shifted from the northeast to the southwest and then from the northwest to the southeast. (3) The AAT, PD, and RR from 2006 to 2020 were all statistically significant at p < 0.01. Notably, the RR positively influences the CCD of WLR, whereas the AAT and PD exert a negative impact. In contrast, the UR and WRUR do not significantly affect the CCD of WLR.
Effects of Different Microplastics on Methane Production and Microbial Community Structure in Anaerobic Digestion of Cattle Manure
Authors: Zhang Mengjiao , Zhao Congxu , Yuan Tian , Wang Qing , Zhang Qiuxian , Yan Shuangdui , Guo Xiaohong , Cao Yanzhuan , Cheng Hongyan
Published: January 2025

Microplastics (MPs) are widely distributed in the environment, and they inevitably enter animal bodies during livestock and poultry farming, leading to their presence in livestock and poultry manure. However, there is limited research on the effects of different types of MPs on the anaerobic digestion (AD) performance of livestock and poultry manure. Herein, we investigated the impact of four types of MPs (polyvinyl chloride (PVC), polypropylene (PP), polyethylene (PE), and polyhydroxyalkanoate (PHA)) on AD performance using cattle manure as a substrate. Results demonstrated that the cumulative methane production in the PE group reached 5568.05 mL, exhibiting an 11.97% increase compared to the control group. Conversely, the cumulative methane production was decreased by 5.52%, 9.69%, and 14.48% in the PP, PVC, and PHA groups, respectively. Physicochemical analyses showed that MPs promoted organic matter hydrolysis on day 4 of AD, leading to the accumulation of volatile fatty acids (VFAs) in the initial stage. Specifically, the acetic acid content of PE was 44.48–92.07 mL/L higher than that of the control during the first 8 days. PE MPs also enriched microorganisms associated with methane production. The abundance of Firmicutes was enhanced by 2.89–17.57%, Methanosaeta by 8.42–12.48%, and Methanospirillum by 10.91–16.89% in comparison to the control; whereas PHA MPs decreased the abundance of Methanosaeta by 8.14–31.40%. Moreover, PHA MPs inhibited methane production by suppressing acetate kinase activity while promoting lactate dehydrogenase release from microorganisms involved in the AD process. Based on changes observed in key enzyme functional gene abundances, PHA MPs reduced acetyl-CoA carboxylase functional gene abundance, negatively affecting the acetone cleavage methanogenesis pathway. Meanwhile, PE MPs significantly increased acetate-CoA ligase abundance, thereby promoting the acetic acid methanogenesis pathway. The results provide novel insights into the influence exerted by MPs on AD performance when applied to livestock manure.
Soft Error-Tolerant and Highly Stable Low-Power SRAM for Satellite Applications
Authors: Oh Jong-Yeob , Jo Sung-Hun
Published: January 2025

As CMOS technology has advanced, the transistor integration density of static random-access memory (SRAM) cells has increased. This has led to a reduction in the critical charge of sensitive nodes, making the SRAM cells more susceptible to soft errors. When high-energy particles in space strike the sensitive nodes of an SRAM cell, a single-event upset (SEU) can occur, altering the stored data. Additionally, the charge-sharing effect between transistors can cause single-event multi-node upsets (SEMNUs). To address these challenges, this paper proposes a radiation-hardened 16T SRAM cell optimized for stability and power, referred to as RHSP16T. The performance of the proposed RHSP16T cell was compared with other radiation-hardened SRAM cells, including QUC-CE12T, WE-QUATRO, RHBD10T, RHD12T, and RSP14T. Simulation results indicate that the proposed RHSP16T cell exhibits higher read and write stability, along with lower-leakage power consumption. compared with all other cells. This demonstrates that RHSP16T ensures high reliability for stored data. Furthermore, EQM results show that the RHSP16T cell outperformed the compared designs in overall SRAM cell performance. The proposed integrated circuit was implemented in a 90 nm CMOS process and operated on 1 V supply voltage.
Evaluating the Thymoquinone Content and Antioxidant Properties of Black Cumin (Nigella sativa L.) Seed Oil During Storage at Different Thermal Treatments
Authors: Neunert Grażyna , Kamińska Wiktoria , Nowak-Karnowska Joanna
Published: January 2025

Black cumin seeds (Nigella sativa) and black cumin seed oil (BCSO) exhibit various pharmacological activities, most of which are attributed to the presence of thymoquinone (TQ). TQ, however, is characterized by low stability at elevated temperatures and instability in aqueous environments. In this study, the spectroscopic properties of TQ were used to monitor changes in TQ content in BCSO subjected to thermal exposure. Simultaneously, the influence of the presence of TQ on the antioxidant properties of this oil was determined. The used spectrofluorimetric and chromatographic method quantified the presence of TQ. The antiradical properties of the oil in different stages of thermal oxidation degradation were determined by the DPPH method. The measured antiradical activity of the oil, depending on the exposure conditions used, revealed the difference correlated with the content of the TQ. However, the presence in BCSO of other bioactive components, like phenols, had a more significant influence on its total antioxidant capacity. Furthermore, our study, for the first time, focused on the rise in TQ content in the oil during thermal storage, indicating a new method to enhance the TQ content in BCSO.
Valorization of Underutilized Mandarin Juice Byproduct Through Encapsulation of Flavonoids Using Freeze-Drying Technique
Authors: Banožić Marija , Filipović Adrijana , Ištuk Jozo , Kovač Mario , Ivanković Anita , Kajić Nikolina , Puljić Leona , Aladić Krunoslav , Jokić Stela
Published: January 2025

This study evaluated Maltodextrin (MD), Gum Arabic (GA), and Carboxymethylcellulose (CMC) in different ratios as coating materials to encapsulate citrus pomace phenolic compounds. Citrus encapsulates were obtained by ultrasound-assisted extraction followed by the freeze-drying process and were characterized regarding the microencapsulation efficiency, physical, and chemical properties. Carrier material choice reflected a significant effect on encapsulation efficiency, phenolic compounds retention, and reconstitution properties of encapsulated extract. The encapsulation efficiency of prepared encapsulates ranked from 50.909% to 84.000%, and it was strongly dependent upon CMC addition. A wide range of reconstitution parameters (water absorption index-WAI and water solubility index-WSI) suggested possible release mechanism modifications. HPLC analysis revealed the presence of three main phenolic compounds, namely Hesperidin, Naringin, and Rutin. A wall material mixture of MD, GA, and CMC in the same proportions was optimal for freeze-drying. This combination resulted in encapsulates with a low moisture content (1.936 ± 0.012%) and a low water activity (0.110 ± 0.001), indicating prolonged stability. Based on the obtained results, freeze-drying as an encapsulation technique should be considered as a promising solution to recover compounds from industry byproducts and protect them from environmental and gastrointestinal circumstances.
A Voting-Based Star Identification Algorithm Using a Partitioned Star Catalog
Authors: He Xu , Zhang Lei , He Jiawei , Mu Zhiya , Lv Zhuang , Wang Jun
Published: January 2025

With the rapid advancement of aerospace technology, the maneuverability of spacecraft has increasingly improved, creating a pressing demand for star sensors with a high attitude update rate and high precision. Star identification, as the most complex and time-consuming algorithm of star sensors, faces stringent requirements for enhanced identification speed and an enhanced identification rate. Furthermore, as the space environment is becoming more complex, the need for star sensors with heightened detection sensitivity is growing to facilitate real-time and accurate alerts for various non-cooperative targets, which has led to a sharp increase in the number of high-magnitude navigation stars in the star catalog, significantly impeding the speed and rate of star identification. Traditional methods are no longer adequate to meet the current demand for star sensors with high identification speed and a high identification rate. Addressing these challenges, a voting-based star identification algorithm using a partitioned star catalog is proposed. Initially, a uniform partitioning method for the star catalog is introduced. Building on this, a navigation feature library using partitioned catalog neighborhoods as a basic unit is constructed. During star identification, a method based on a voting decision is employed for feature matching in the basic unit. Compared to conventional methods, the proposed algorithm significantly simplifies the navigation feature library and narrows the retrieval region during star identification, markedly enhancing identification speed while effectively reducing the probability of redundant and false matching. The performance of the proposed algorithm is validated through a simulation experiment and nighttime star observation experiment. Experimental results indicate an average identification rate of 99.760% and an average identification time of 8.861 milliseconds, exhibiting high robustness against position errors, magnitude errors, and false stars. The proposed algorithm presents a clear advantage over other common star identification methods, meeting the current requirement for star sensors with high star identification speed and a high identification rate.
On Value Distribution for the Mellin Transform of the Fourth Power of the Riemann Zeta Function
Authors: Garbaliauskienė Virginija , Rimkevičienė Audronė , Stoncelis Mindaugas , Šiaučiūnas Darius
Published: January 2025

In this paper, the asymptotic behavior of the modified Mellin transform Z2(s), s=σ+it, of the fourth power of the Riemann zeta function is characterized by weak convergence of probability measures in the space of analytic functions. The main results are devoted to probability measures defined by generalized shifts Z2(s+iφ(τ)) with a real increasing to +∞ differentiable functions connected to the growth of the second moment of Z2(s). It is proven that the mass of the limit measure is concentrated at the point expressed as h(s)≡0. This is used for approximation of h(s) by Z2(s+iφ(τ)).
A Recursive Attribute Reduction Algorithm and Its Application in Predicting the Hot Metal Silicon Content in Blast Furnaces
Authors: Li Zhanqi , Cheng Pan , Yin Linzi , Guan Yuyin
Published: January 2025

For many complex industrial applications, traditional attribute reduction algorithms are often inefficient in obtaining optimal reducts that align with mechanistic analyses and practical production requirements. To solve this problem, we propose a recursive attribute reduction algorithm that calculates the optimal reduct. First, we present the notion of priority sequence to describe the background meaning of attributes and evaluate the optimal reduct. Next, we define a necessary element set to identify the “individually necessary” characteristics of the attributes. On this basis, a recursive algorithm is proposed to calculate the optimal reduct. Its boundary logic is guided by the conflict between the necessary element set and the core attribute set. The experiments demonstrate the proposed algorithm’s uniqueness and its ability to enhance the prediction accuracy of the hot metal silicon content in blast furnaces.
Editorial for the Special Issue on “Multidisciplinary Insights on Bone Healing (Volume II)”
Authors: Anesi Alexandre , Di Bartolomeo Mattia
Published: January 2025

As the population ages and differences among sexes and age groups become more pronounced, the research on bone healing and damage mechanisms continues to advance, with evaluation conducted in both pre-clinical and clinical settings [...]
Targeting Cutaneous Leishmaniasis with Thiadiazine Thione Derivatives: An In Vivo Study of Its Anti-Inflammatory, Anti-Pyretic, Anti-Nociceptive, and Anti-Sedative Properties
Authors: Sarwar Sarah , Sarwar Nadia , Ali Haleema , Rafiullah  , Khan Rasool , Ahmad Ajaz , Ullah Amin , Soonmin Ho , Ullah Nazif
Published: January 2025

Background/Objectives: Thiadiazine thione (THTT) has gained significant interest owing to its pharmacological potentials, particularly its antiparasitic and anti-inflammatory properties. Leishmaniasis is a clinical syndrome caused by infection with Leishmania species and is associated with an inflammatory response and nociception. The available treatments against leishmaniasis are inadequate, as they are associated with high cost, toxicity, and increased resistance. Methods: In the current study, the antileishmanial potential of five Thiadiazine thione derivatives (C1–C5) was evaluated in vivo against Leishmania tropica. Experiments were performed on BALB/c mice infected with promastigotes and treated with THTT derivatives for 15 days. Additionally, the derivatives were evaluated for their anti-inflammatory, antinociceptive, antipyretic, and antisedative properties using standardized models, including carrageenan-induced paw edema, acetic acid-induced abdominal writhes, yeast-induced fever, and white wood apparatus, respectively. Results: Of the tested derivatives, C5 exhibited the most promising results, with a 61.78% reduction in lesion size and significant decrease in parasite load. Among the derivatives, C1 showed the highest anti-inflammatory activity, with 63.66% inhibition in the paw edema test at the 5th hour post treatment. In the antipyretic assay, C1 and C5 were able to reduce body temperature to a normal level within 1 h of treatment. Furthermore, compounds C4, C2, and C1 showed high nociceptive activity, while C1 and C5 demonstrated the most notable antisedative effects (94 ± 2 and 92 ± 1, respectively), outperforming the standard drug diazepam (13 ± 1). Conclusion: These in vivo findings suggest that THTT derivatives have the potential to serve as a template for developing leishmanicidal drugs, with added anti-inflammatory and analgesic properties.
Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells
Authors: Hai Qimin , Bazeley Peter , Han Juying , Brubaker Gregory , Powers Jennifer , Diaz-Montero Claudia M. , Smith Jonathan D.
Published: January 2025

Background/Objectives: Macrophages play a pivotal role in various pathogenic processes, necessitating the development of efficient differentiation techniques to meet the high demand for these cells in research and therapy. Human macrophages can be obtained via culturing peripheral blood monocytes; however, this source has limited yields and requires patient contact for each proposed use. In addition, it would be difficult to perform gene editing on peripheral blood monocytes. The objectives of this study are to define a robust and consistent method for the differentiation of induced pluripotent stem cells (iPSCs) into macrophages that can address these needs for recurrent studies with high yields and the potential for gene editing. Methods: We refined the traditional embryoid body-based differentiation strategy to create a novel three-phase method that optimizes yield, consistent quality, and reproducibility. This approach incorporates microwell plates and cell filtration to standardize the production of embryoid bodies and subsequent macrophage progenitors. Using up to five independent iPSC donors, we performed several assays for macrophage functions and polarization, such as marker protein staining by flow cytometry, lipoprotein uptake, phagocytosis, cytokine release, inflammasome activation, and the effects of M1-like and M2-like polarization. RNA sequencing was performed to determine the segregation of cells at different stages of differentiation and by iPSC donor, as well as to identify marker genes for each stage of differentiation. Results: The iPSC-derived macrophages generated through this method exhibit characteristic features and cell marker proteins, as well as classical macrophage activities, including lipoprotein uptake, bacterial phagocytosis, cytokine release, and inflammasome activation. We demonstrate the effects of M1-like and M2-like polarization on cytokine release. The first three principal components of the RNA sequencing data showed clear clustering by differentiation stage. In contrast, the fourth and fifth principal components clustered the differentiated macrophages by their respective iPSC donor. Marker genes were identified for each stage of differentiation and polarization. Conclusions: The methods provide an optimized and simplified procedure to produce iPSC-derived macrophages. Our results demonstrate the reproducibility of this method in generating high-quality macrophages suitable for a variety of biomedical applications.
Eugenia uniflora Effects on the Depressive-like Behavior of MPTP-Exposed Female Rats: Apoptosis and α-Synuclein Modulation
Authors: Savall Anne Suély Pinto , De Mello Jhuly Dorneles , Fidelis Eduarda Monteiro , Bortolotto Vandreza Cardoso , Dahleh Mustafa Munir Mustafa , Guerra Gustavo Petri , Prigol Marina , Puntel Robson , Boldori Jean Ramos , Denardin Cristiane Casagrande , Sampaio Tuane Bazanella , Pinton Simone
Published: January 2025

Background: Parkinson’s disease (PD) is a neurodegenerative disorder marked by motor deficits and non-motor symptoms, such as depression, which are associated with dopaminergic loss and α-synuclein aggregation in the brain. Objectives: This study investigated the neuroprotective effects of a hydroalcoholic extract of the purple fruit of Eugenia uniflora (PFEU) on motor ability and depressive-like behaviors in a PD model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in female Wistar rats. Methods: Rats received intranasal administration of MPTP or vehicle, followed by 14 days of oral administration of PFEU (300 or 2000 mg/kg, administered once daily) or vehicle. Depressive-like behavior was assessed using the splash and forced swimming tests, while motor ability was evaluated using the rotarod and open field tests. On day 15, hippocampal tissue was collected for immunoreactivity analysis. Results: MPTP treatment induced depressive-like behavior, which was significantly reversed by PFEU, as evidenced by increased grooming and decreased immobility. No motor coordination or locomotion deficits were observed. Furthermore, PFEU treatment prevented the MPTP-induced increase in hippocampal α-synuclein, p-p53, and Bax while restoring Bcl-2 levels, suggesting neuroprotective effects through the modulation of apoptotic pathways and α-synuclein. Conclusions: These findings support PFEU’s potential as a neuroprotective agent for MPTP-induced depressive-like behavior in female rats, highlighting its molecular mechanisms.
An Optimization Study on Continuous Steel Box Girder Bridge Components
Authors: Wang Ang , Gao Ruiyuan , Chen Qingfeng , Jin Weizhun , Fang Pengfei , Wu Di
Published: January 2025

The steel box girder bridge is a structure composed of mutually vertical stiffening ribs (longitudinal ribs and transverse ribs) that carry the loads of vehicles. Since the external loads are usually complex and variable, the rational design of the bridge components is a topic that deserves more attention. The purpose of this study is to explore the optimal range of some of the component design parameters, expecting to reduce costs while ensuring the stress-carrying capacity. A finite element model (FEM) based on ABAQUS was built and the results were verified by laboratory experiments. The varied thicknesses of the bridge deck, diaphragm, and U-rib were explored based on the validated FEM. The simulation results fit well with the experimental results, which proved that the FEM was quite reliable. The stress analysis results demonstrated an optimal range of 18–20 mm for bridge deck thickness, 14–16 mm for diaphragm thickness, and 8–10 mm for U-rib thickness. The present study holds significant reference value for the design and optimization of multiple steel box girder bridge components, which could further provide a theoretical foundation for related research in this field.
Integrating Building Information Modelling into Construction Project Management Education in Australia: A Comprehensive Review of Industry Needs and Academic Gaps
Authors: Papuraj Xavier , Izadyar Nima , Vrcelj Zora
Published: January 2025

Integrating Building Information Modelling (BIM) into Construction Project Management (CPM) curricula is crucial for preparing industry-ready professionals with the digital competencies needed in the rapidly evolving, technology-driven construction sector. This systematic literature review evaluated gaps and challenges in BIM education within CPM courses, including limited faculty training, inconsistent curricula, and insufficient hands-on, interdisciplinary collaboration opportunities for students. These deficiencies hinder consistent BIM competency development among graduates, resulting in disparities in skill levels and readiness for industry demands. This study identified essential digital management skills and BIM competencies required for effective industry practice by examining global academic research. The findings revealed that despite advancing BIM adoption, significant gaps persist in its teaching, particularly the lack of collaborative education within project management disciplines, and need for enhanced collaboration between academia and the industry to bridge the skills gap. Industry professionals and academics emphasise the deficit in BIM knowledge among project management graduates and advocate for a cohesive educational framework aligning with industry requirements, emphasising hands-on experience and interdisciplinary collaboration. This study highlighted significant gaps and opportunities for integrating Building Information Modelling (BIM) into Construction Project Management (CPM) education, with the aim to enhance the competency and employability of future construction project managers. By proposing a phased approach and a BIM educational framework tailored to the Australian context, this review recommended the integration of BIM, supported by other emerging technologies, to better align educational outcomes with industry demands. The recommendations focus on curriculum design and implementation strategies to bridge the identified gaps.
Usefulness of Serum Biomarkers in Predicting Anastomotic Leakage After Gastrectomy
Authors: Ramos Diego , Gallego-Colón Enrique , Mínguez Javier , Bodega Ignacio , Priego Pablo , García-Moreno Francisca
Published: January 2025

Background/Objectives: Anastomotic leakage (AL) is one of the most concerning complications following gastrectomy. The aim of this study was to assess and compare the predictive accuracy of C-reactive protein (CRP), procalcitonin (PCT), the neutrophil-to-lymphocyte ratio (NLR), the platelet-to-lymphocyte ratio (PLR), fibrinogen, and the mean platelet volume (MPV) in the early diagnosis of post-gastrectomy AL. Methods: A prospective bicentric observational study was conducted including all patients undergoing elective gastrectomy between August 2018 and December 2022. The performance of the selected biomarkers in predicting the existence of AL within the first 7 postoperative days (PODs) was assessed. Results: A total of 107 patients were included for analysis. The incidence of AL was 20.56%, and the median day of diagnosis was on POD5 (interquartile range 4–6). CRP, PCT, the NLR, the PLR, and fibrinogen showed significant associations with the presence of AL (from POD2 for CRP and fibrinogen and from POD3 for PCT, NLR, and PLR). CRP demonstrated a superior predictive accuracy on POD4, with a threshold value of 181.4 mg/L (NPV 99%; AUC 0.87, p < 0.001); PCT demonstrated a superior predictive accuracy on POD7, with a threshold value of 0.13 μg/L (NPV 98%; AUC 0.84, p < 0.001); the NLR showed a superior predictive accuracy on POD6, with a threshold ratio of 6.77 (NPV 95%; AUC 0.86, p < 0.001); the PLR achieved a superior predictive accuracy on POD7, with a ratio of 234 (NPV 98%; AUC 0.71; p = 0.002); and fibrinogen demonstrated a superior predictive accuracy on POD5, with a threshold of 7.344 g/L (NPV 98%; AUC 0.74; p = 0.003). In the comparison of predictive accuracy, CPR, PCT, and the NLR were found to be superior to all other biomarkers. Conclusions: CRP, PCT, and the NLR are biomarkers with a sufficient predictive ability to clinically discard the presence of AL within the first postoperative week.
Gastric Intestinal Metaplasia in Children and Adolescents Is Reversible upon Reaching Adulthood—Results from a Long-Term Cohort Study
Authors: Drnovšek Jan , Zidar Nina , Jeruc Jera , Šmid Lojze M. , Vidmar Gaj , Štabuc Borut , Homan Matjaž
Published: January 2025

Background/Objectives: Gastric intestinal metaplasia (GIM) is considered an irreversible preneoplastic precursor for gastric adenocarcinoma in adults. However, its significance in children and the long-term outcome remain poorly understood. Methods: All children diagnosed with GIM between 2000 and 2020 were identified at a large tertiary referral centre. Upon reaching adulthood (≥18 years), the patients were invited to undergo follow-up esophagogastroduodenoscopy (using narrow-band imaging additionally to high-definition white light endoscopy), with gastric biopsies obtained according to the updated Sydney protocol. Childhood and adulthood gastric biopsies were re-evaluated by two experienced gastrointestinal pathologists using Kreyberg staining. Results: Paediatric GIM was diagnosed in 178/14,409 (1.2%) esophagogastroduodenoscopies performed during the study period. Fifty adult patients with childhood GIM agreed to participate in the study. The mean age at childhood and adulthood endoscopies were 14.3 years (median 15) and 25.2 years (median 24), respectively. The mean follow-up interval was 10.5 years. All childhood GIM cases were classified as complete-type. Notably, GIM completely resolved in 41/50 of patients (82%) by the time of adulthood follow-up. No dysplasia or carcinoma was detected in any patient. Childhood Helicobacter pylori infection, similar to other evaluated host-related factors, was not significantly associated with the persistence of GIM into adulthood (11.2% vs. 29.3%, p = 0.41). Conclusions: Childhood GIM was a rare finding but demonstrated a high rate of reversibility by adulthood regardless of Helicobacter pylori status, with no cases of dysplasia or carcinoma observed during long-term follow-up.
Exploring the Metabolic Impact of FLASH Radiotherapy
Authors: Geirnaert Febe , Kerkhove Lisa , Montay-Gruel Pierre , Gevaert Thierry , Dufait Inès , De Ridder Mark
Published: January 2025

FLASH radiotherapy (FLASH RT) is an innovative modality in cancer treatment that delivers ultrahigh dose rates (UHDRs), distinguishing it from conventional radiotherapy (CRT). FLASH RT has demonstrated the potential to enhance the therapeutic window by reducing radiation-induced damage to normal tissues while maintaining tumor control, a phenomenon termed the FLASH effect. Despite promising outcomes, the precise mechanisms underlying the FLASH effect remain elusive and are a focal point of current research. This review explores the metabolic and cellular responses to FLASH RT compared to CRT, with particular focus on the differential impacts on normal and tumor tissues. Key findings suggest that FLASH RT may mitigate damage in healthy tissues via altered reactive oxygen species (ROS) dynamics, which attenuate downstream oxidative damage. Studies indicate the FLASH RT influences iron metabolism and lipid peroxidation pathways differently than CRT. Additionally, various studies indicate that FLASH RT promotes the preservation of mitochondrial integrity and function, which helps maintain apoptotic pathways in normal tissues, attenuating damage. Current knowledge of the metabolic influences following FLASH RT highlights its potential to minimize toxicity in normal tissues, while also emphasizing the need for further studies in biologically relevant, complex systems to better understand its clinical potential. By targeting distinct metabolic pathways, FLASH RT could represent a transformative advance in RT, ultimately improving the therapeutic window for cancer treatment.
p63: A Master Regulator at the Crossroads Between Development, Senescence, Aging, and Cancer
Authors: Sadu Murari Lakshana Sruthi , Kunkel Sam , Shetty Anala , Bents Addison , Bhandary Aayush , Rivera-Mulia Juan Carlos
Published: January 2025

The p63 protein is a master regulatory transcription factor that plays crucial roles in cell differentiation, adult tissue homeostasis, and chromatin remodeling, and its dysregulation is associated with genetic disorders, physiological and premature aging, and cancer. The effects of p63 are carried out by two main isoforms that regulate cell proliferation and senescence. p63 also controls the epigenome by regulating interactions with histone modulators, such as the histone acetyltransferase p300, deacetylase HDAC1/2, and DNA methyltransferases. miRNA-p63 interactions are also critical regulators in the context of cancer metastasis. This review aims to elaborate on the diverse roles of p63, focusing on disease, development, and the mechanisms controlling genome organization and function.
Publisher’s Note: Journal of Craniomaxillofacial Trauma and Reconstruction (CMTR)
Authors: Roth Peter
Published: January 2025

We are excited to welcome the Journal of Craniomaxillofacial Trauma and Reconstruction (CMTR) to the MDPI family [...]
The Influence of an Alternating Current Field on Pack Boriding for Medium Carbon Steel at Moderate Temperature
Authors: Li Xiaoxiao , Liu Wei , Yuan Jianguang , Yuan Jiaye , Zhou Xiaobao , Pan Taijun , Ren Yanjie
Published: January 2025

The influence of alternating current (AC) field on the pack boriding process for medium carbon steel was investigated through characterization of microstructure, phase composition, microhardness, and corrosion resistance of the boride layer and its mechanism was revealed. Results showed that the boride layer obtained by AC field boriding was composed of the outer FeB and the inner Fe2B phase, which was similar to that of conventional boriding. Meanwhile, the effective thickness of the boride layer and proportion of Fe2B increased gradually with increasing current during AC field boriding. The introduction of an AC field during the boriding process served dual purposes. First, it facilitated the decomposition of the boriding medium, leading to an elevation in the concentration of active boron atoms. Second, it reduced the activation energy required for atomic diffusion, thereby accelerating the diffusion of both boron and iron atoms. These combined effects significantly enhanced the hardness distribution and corrosion resistance of the steel. Further insights into the process were gained by fitting the parabolic kinetics curves, which confirmed that the boriding process in an AC field was exclusively controlled by diffusion. This study also clarified the growth mechanism of the boride layer within an AC field.
Silver Nanoparticles–Chitosan Nanocomposites as Protective Coatings for Dental Remineralization Treatment: An In Vitro Study
Authors: de Rodríguez Katleen A. Aguirre , de González Wendy Y. Escobar , Castañeda Monroy Vianney , Murphy Sean , Martínez-Castañón Gabriel-Alejandro , Bach Horacio , Niño-Martínez Nereyda
Published: January 2025

Research with nanoparticles for the treatment and prevention of dental caries is of special interest given the high prevalence of the disease worldwide. Several studies support the use of nanoparticles associated with materials given their antimicrobial properties and potential demineralization reduction. This study aimed to evaluate the impact of the application of silver nanoparticles (AgNPs) and chitosan gel in combination with commercial fluoride varnish on the remineralization of dental enamel. Ninety-six tooth blocks were macroscopically evaluated via stereomicroscopy, ICDAS II, and laser fluorescence. Enamel blocks were subjected to artificial demineralization and divided into four exposure groups (24, 48, 120, and 168 h), and five different remineralizing agents were applied, namely, FV (fluoride varnish), FV + CG (fluoride varnish + chitosan gel), FV + AgNPs (fluoride varnish + AgNPs), FV + AgNPs + CG (fluoride varnish + AgNPs + chitosan gel), and AgNPs + CG (AgNPs + chitosan gel). Enamel surface changes were evaluated via laser fluorescence, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Laser fluorescence results obtained from demineralized blocks and subsequently exposed to remineralizing treatment indicate significant differences. After exposure to remineralizing agents, hydroxyapatite and modified apatite phases were identified mainly in the samples treated with FV + AgNPs + CG in the groups exposed for 24, 48, and 120 h. The FV + AgNPs + CG and AgNPs + CG indicate good performance in terms of the Ca/P ratio in in vitro demineralization compared to the group treated with fluorine varnish.
Enhancement of the Corrosion and Wear Resistance of an Epoxy Coating Using a Combination of Mullite Powder and PVB
Authors: Zhao Yan , Ma Huachao , Gao Zhenglu , Huang Ziyan , Wu Yuanyuan , Lv Kuilin
Published: January 2025

Currently, with the gradual development of corrosion-resistant materials, coatings often exhibit ultra-high hydrophobic properties while possessing corrosion resistance, complicating the preparation of corrosion-resistant coatings. To explore a novel coating that combines high corrosion resistance with simplified preparation methods, mullite/kaolin powder was stirred using ball milling, and polyvinyl butyral was added to serve as a binder, thereby preparing a hydrophilic and highly corrosion-resistant coating. The coating was characterized using SEM, IR, XRD, and other testing methods. The results revealed that the components of the coating are connected through physical crosslinking, avoiding chemical reactions. Regarding the coating’s performance, electrochemical and salt spray tests were conducted to characterize the prepared coating. According to electrochemical impedance spectroscopy tests, after immersion for 7 days, the electrochemical impedance spectroscopy impedance value of the A4C6EP coating reached 1.13 × 108 Ω·cm2, several times higher than that of other coatings, demonstrating its superior corrosion resistance. After a salt spray test for 2000 h, the coating surface showed neither bubbles, further validating the excellent corrosion resistance of the A4C6EP coating. The A4C6EP coating underwent an abrasion test using sandpaper and, after 100 cycles, the contact angle decreased by only 2.3°, with only slight scratches appearing on the surface, indicating very high mechanical abrasion resistance. This research demonstrates the successful preparation of a hydrophilic coating with excellent corrosion resistance and ultra-high mechanical abrasion resistance through a simple method, providing new insights for the development of hydrophilic corrosion-resistant coatings and reducing the cost of such coatings.
Therapeutic Capabilities of Triterpenes and Triterpenoids in Immune and Inflammatory Processes: A Review
Authors: Mantiniotou Martha , Athanasiadis Vassilis , Kalompatsios Dimitrios , Bozinou Eleni , Lalas Stavros I.
Published: January 2025

Plant-derived secondary metabolites such as triterpenes and triterpenoids are present in a wide range of plant species. These compounds are particularly attractive due to their extensive range of biological properties and potential applications as intermediates in the synthesis of novel pharmacologically promising medications. Saponins, which are glycosylated triterpenoids found in nature, exhibit the same properties. At this point, the effectiveness of saponins as an anti-inflammatory medication has been verified. This review article examines the primary connections between immune responses and anti-inflammatory activity, focusing specifically on the correlation between triterpenes and triterpenoids. These connections have been investigated in various cell models, as well as in vitro and in vivo studies. The present research provides a comprehensive overview of the current understanding of the therapeutic capabilities of triterpenes and triterpenoids in immune and inflammatory processes. It also highlights emerging standards and their potential utilization in pharmaceutical and clinical settings.
Computer-Aided Efficient Routing and Reliable Protocol Optimization for Autonomous Vehicle Communication Networks
Authors: Dafhalla Alaa Kamal Yousif , Elobaid Mohamed Elshaikh , Tayfour Ahmed Amira Elsir , Filali Ameni , SidAhmed Nada Mohamed Osman , Attia Tahani A. , Mohajir Badria Abaker Ibrahim , Altamimi Jawaher Suliman , Adam Tijjani
Published: January 2025

The rise of autonomous vehicles necessitates advanced communication networks for effective data exchange. The routing protocols Ad hoc On-Demand Distance Vector (AODV) and Greedy Perimeter Stateless Routing (GPSR) are vital in mobile networks (MANETs) and vehicular ad hoc networks (VANETs). However, their performance is affected by changing network conditions. This study examines key routing parameters—MaxJitter, Hello/Beacon Interval, and route validity time—and their impact on AODV and GPSR performance in urban and highway scenarios. The simulation results reveal that increasing MaxJitter enhances AODV throughput by 12% in cities but decreases it by 8% on highways, while GPSR throughput declines by 15% in cities and 10% on highways. Longer Hello intervals improve AODV performance by 10% in urban settings but reduce it by 6% on highways. Extending route validity time increases GPSR’s Packet Delivery Ratio (PDR) by 10% in cities, underscoring the need to optimize routing parameters for enhanced VANET performance.
Potential of Cissampelos pareira L. Pectin as a Bioactive Compound in Moisturizing and Anti-Aging Applications
Authors: Chanpirom Setinee , Khat-udomkiri Nuntawat , Tree-Udom Thapakorn , Ditthawutthikul Naphatsorn , Saewan Nisakorn , Vinardell Maria , Sripisut Tawanun
Published: January 2025

Cissampelos pareira is a plant known for its edible and medicinal properties, with bioactive potential in various applications. This study aimed to isolate and characterize pectin from C. pareira (PCP) and assess its safety, as well as its efficacy in skin moisturizing and anti-aging effects on human volunteers. The highest yield of PCP extraction was achieved after a 45 min extraction period. Chemical composition analysis using Fourier transform infrared (FT-IR) spectroscopy confirmed the presence of a characteristic pectin structure. Optimized PCP samples revealed high methoxy pectin content, degree of esterification, and galacturonic acid concentration, along with consistent physicochemical properties. PCP exhibited antioxidant activity and showed no cytotoxicity at 1 mg/mL, and effectively reduced hydrogen peroxide-induced oxidative damage in NIH/3T3 cells. Stability assessment indicated that PCP maintains its physical appearance and total polysaccharide content after heating–cooling periods. None of the PCP formulations caused skin irritation among volunteers. In an 8-week clinical trial, subjects who used serum formulations containing 0.5% w/w and 1.0% w/w PCP showed significant improvements in skin hydration and elasticity compared to the placebo serum group. While skin smoothness, scaliness, and wrinkling also improved, these anti-aging results were not statistically significant. The results indicated PCP is a promising candidate for skin care applications due to its safety, stability, antioxidant activity, and demonstrated efficacy in enhancing skin hydration and elasticity.
Sarcopenia Index Is Correlated with Osteoporosis in Patients with Chronic Kidney Disease
Authors: Kim Segi , Jeong Simho , Kim Kyeongmi , Sung Junhee , Kim Do Kyung , Lee Soonchul
Published: January 2025

Objectives: This study aimed to investigate the relationship between the sarcopenia index (SI), which is derived from serum creatinine and cystatin C levels, and osteoporosis in chronic kidney disease (CKD). Methods: This study initially included patients who underwent dual-energy X-ray absorptiometry (DXA) and serum creatinine and cystatin C testing between 2005 and 2022. Subsequently, patients diagnosed with CKD were selected for the final analysis, totaling 102 patients. Both traditional and new SI were calculated, with each participant categorized into one of two groups (non-osteoporosis and osteoporosis) according to bone mineral density. To enhance statistical validity, the patients were further divided into low- and high-index groups based on the median value of both indices for comparative analysis. The association between SI and the risk of osteoporosis was estimated using multivariable logistic regression analysis. Results: Participants with lower SI values had lower bone mineral density and a higher diabetes mellitus prevalence. The non-osteoporotic group exhibited significantly higher mean values for both traditional and new SI. Multivariable logistic regression analysis identified three statistically significant variables: both indices, sex, and diabetes mellitus. Both traditional and new SI yielded individual odds ratios of 0.906 with estimated areas under the curve of 0.847 for traditional SI and 0.833 for new SI. Conclusions: This study confirmed that both traditional and new SI are associated with osteoporosis in patients with CKD. Therefore, clinicians can raise the suspicion of osteoporosis based on traditional and new SI in patients with CKD, even when DXA results are unavailable.
Skin Cancer Detection Using Transfer Learning and Deep Attention Mechanisms
Authors: Alotaibi Areej , AlSaeed Duaa
Published: January 2025

Background/Objectives: Early and accurate diagnosis of skin cancer improves survival rates; however, dermatologists often struggle with lesion detection due to similar pigmentation. Deep learning and transfer learning models have shown promise in diagnosing skin cancers through image processing. Integrating attention mechanisms (AMs) with deep learning has further enhanced the accuracy of medical image classification. While significant progress has been made, further research is needed to improve the detection accuracy. Previous studies have not explored the integration of attention mechanisms with the pre-trained Xception transfer learning model for binary classification of skin cancer. This study aims to investigate the impact of various attention mechanisms on the Xception model’s performance in detecting benign and malignant skin lesions. Methods: We conducted four experiments on the HAM10000 dataset. Three models integrated self-attention (SL), hard attention (HD), and soft attention (SF) mechanisms, while the fourth model used the standard Xception without attention mechanisms. Each mechanism analyzed features from the Xception model uniquely: self-attention examined the input relationships, hard-attention selected elements sparsely, and soft-attention distributed the focus probabilistically. Results: Integrating AMs into the Xception architecture effectively enhanced its performance. The accuracy of the Xception alone was 91.05%. With AMs, the accuracy increased to 94.11% using self-attention, 93.29% with soft attention, and 92.97% with hard attention. Moreover, the proposed models outperformed previous studies in terms of the recall metrics, which are crucial for medical investigations. Conclusions: These findings suggest that AMs can enhance performance in relation to complex medical imaging tasks, potentially supporting earlier diagnosis and improving treatment outcomes.
The Interspecific Abundance–Occupancy Relationship in Invertebrate Metacommunities Associated with Intertidal Mussel Patches
Authors: Scrosati Ricardo A.
Published: January 2025

To explain the distribution and abundance of species, ecology searches for general models. A pattern often encountered in nature is the interspecific abundance–occupancy relationship (AOR), which describes how the mean local abundance of species relates to the proportion of local sites that each species occupies. Both are central variables in ecology and are often positively correlated, although exceptions have been found. As most AOR research has been conducted with terrestrial systems, recent studies are testing for its occurrence in marine systems. This contribution tests the AOR for invertebrate metacommunities associated with intertidal mussel patches. Using data from six coastal locations in Nova Scotia (Canada), this study shows that the negative binomial model properly describes the relationship between abundance and occupancy for these systems. The degree of wave exposure (wave-sheltered versus wave-exposed habitats) had some influence on the shape of the AOR. Overall, these findings extend the applicability of the AOR to intertidal invertebrate metacommunities. The raw data are included as part of this article to help future syntheses on the AOR, which will need data for a variety of terrestrial and aquatic environments.