bluuebunny/crossref_metadata_2025 huggingface dataset:
Sclerostin Modulation Holds Promise for Dental Indications
Authors: Hassan Mohamed G. , Zaher Abbas R. , Palomo Juan Martin , Palomo Leena
Published: November 2018

Sclerostin modulation is a novel therapeutic bone regulation strategy. The anti-sclerostin drugs, proposed in medicine for skeletal bone loss may be developed for jaw bone indications in dentistry. Alveolar bone responsible for housing dentition share common bone remodeling mechanisms with skeletal bone. Manipulating alveolar bone turnover can be used as a strategy to treat diseases such as periodontitis, where large bone defects from disease are a surgical treatment challenge and to control tooth position in orthodontic treatment, where moving teeth through bone in the treatment goal. Developing such therapeutics for dentistry is a future line for research and therapy. Furthermore, it underscores the interprofessional relationship that is the future of healthcare.
LODsyndesis: Global Scale Knowledge Services
Authors: Mountantonakis Michalis , Tzitzikas Yannis
Published: November 2018

In this paper, we present LODsyndesis, a suite of services over the datasets of the entire Linked Open Data Cloud, which offers fast, content-based dataset discovery and object co-reference. Emphasis is given on supporting scalable cross-dataset reasoning for finding all information about any entity and its provenance. Other tasks that can be benefited from these services are those related to the quality and veracity of data since the collection of all information about an entity, and the cross-dataset inference that is feasible, allows spotting the contradictions that exist, and also provides information for data cleaning or for estimating and suggesting which data are probably correct or more accurate. In addition, we will show how these services can assist the enrichment of existing datasets with more features for obtaining better predictions in machine learning tasks. Finally, we report measurements that reveal the sparsity of the current datasets, as regards their connectivity, which in turn justifies the need for advancing the current methods for data integration. Measurements focusing on the cultural domain are also included, specifically measurements over datasets using CIDOC CRM (Conceptual Reference Model), and connectivity measurements of British Museum data. The services of LODsyndesis are based on special indexes and algorithms and allow the indexing of 2 billion triples in around 80 min using a cluster of 96 computers.
Imaging Analysis and Digital Restoration of the Holy Face of Manoppello—Part II
Authors: De Caro Liberato , Matricciani Emilio , Fanti Giulio
Published: November 2018

In this second work on the Holy Face of Manoppello, known also as the Veil, we have performed a spectral analysis of the transmitted image. Its thin linen threads are translucent, presumably because starched, allowing light to pass through them. As a result, the yellowish colour of the ancient linen and starch contribute substantially to the final hues of the Holy Face, especially when the Veil is lit from the backside with grazing light. Spectrophotometry measurements have allowed calculating how the fabric absorbs the various chromatic components. Through these quantitative evaluations, it has been possible to compensate the colours of the transmitted Holy Face image, by subtracting the contribution due to the yellowish coloration of the thin linen threads. Furthermore, the rotational spectrum of the image has been studied after digital restoration. The linear fit of the power spectrum in bi-logarithmic scale with a power law f P provided a slope parameter P = − 3.49 ± 0.03 . This is a surprising value because it is typical of photographs of human faces, not of portraits of human faces painted by artists, which instead have statistical properties of fractal type, with values of slopes P = − 2.0 .
The Influence of Environment in the Alteration of the Stained-Glass Windows in Portuguese Monuments
Authors: Palomar Teresa , Redol Pedro , Cruz Almeida Isabel , Pereira da Silva Eduardo , Vilarigues Marcia
Published: November 2018

This work presents the results of the exposure of soda-lime, potash-lime and mixed-alkali silicate glasses during ten and twenty months in different Portuguese monuments with historical stained-glass windows to characterize the influence of local environmental conditions. The glass samples were exposed in the Monastery of Batalha (Batalha), the Monastery of Jerónimos (Lisbon), and the Cathedral of Évora (Évora). A set of analytical techniques to assess the physicochemical effects were used, including optical microscopy and Fourier transform infrared spectroscopy. All the samples presented crystalline deposits on their surface; however, their quantity and nature depended on the atmospheric conditions during the days before the collection. Potash-lime silicate glass was the most altered glass in comparison with soda-lime and mixed-alkali silicate glasses. The samples from the Cathedral of Évora showed a high content of dust and salts on their surface but without severe chemical pathologies; however, those samples exposed in the Monastery of Jerónimos and the Monastery of Batalha presented alteration layers due to a high humidity environment.
Introducing the HERACLES Ontology—Semantics for Cultural Heritage Management
Authors: Hellmund Tobias , Hertweck Philipp , Hilbring Désirée , Mossgraber Jürgen , Alexandrakis George , Pouli Paraskevi , Siatou Amalia , Padeletti Giuseppina
Published: November 2018

Cultural Heritage (CH) (In the context of this paper, we consider cultural heritage built tangible cultural heritage, such as buildings or monuments.) is an important source of identity for humankind and needs to be conserved for future generations. Climate change (CC) will morph the environmental landscape, thus leading to climate stress imposed on CH. Experts from different domains, including, but not limited to, material scientists, conservators and managers of cultural heritage collaborate to find out how CC affects CH and how potentially harmful impacts can be mitigated. To find and understand correlations and effects of different factors, researchers collect and analyse vast amounts of data. Still, experts often cannot exchange or make efficient use of data since it often is unstructured, incompatible, or its plain existence is simply unknown. This article introduces means to achieve consent about available knowledge, to exploit synergy effects through the combination of available information and to provide a flexible multisource information platform in collaborative cultural heritage management projects. In the context of the European project HERACLES (HERACLES—HEritage Resilience Against CLimate Events on Site. Further information: http://www.heracles-project.eu/), an application-ontology was developed. The ontology facilitates reuse and integration of data through structuring and representing its semantics. The involvement in the HERACLES project guaranteed end-user driven development, practical results and encompassment of all domains represented in the project.
Urinary 8-Hydroxy-2′-deoxyguanosine (8-OHdG) Concentrations and Menstrual Cycle Characteristics in Female University Students
Authors: Konishi Shoko , Yoshinaga Jun , Nishihama Yukiko , Onoda Yu , Chisaki Youichi , Imai Hideki
Published: November 2018

Higher concentrations of oxidative stress biomarkers are found in women with polycystic ovary syndrome (PCOS) and endometriosis, conditions linked to irregular menstrual cycles and menstrual pain. The aim of the present study was to test whether women with higher oxidative stress are more likely to show irregular menstrual cycles and severe menstrual pain compared with women with lower oxidative stress. A cross-sectional study was conducted targeting female university students with a mean (SD) age of 20.5 (1.8) years (n = 188). Participants completed a questionnaire on reproductive characteristics and anthropometry and kept a menstrual cycle diary for 5 consecutive months. Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), cotinine, and creatinine concentrations were measured once during the study period. The mean (SD) value of the urinary 8-OHdG concentration was 4.7 (2.0) μg/g of creatinine. A total of 1021 menstrual cycles were recorded. The participants were categorized as either having regular (68%) or irregular (18%) cycles or oligomenorrhea (13%) or polymenorrhea (1%). The urinary 8-OHdG concentration did not significantly differ across menstrual cycle regularity or pain categories. Even after adjusting for age, body mass index (BMI), and urinary cotinine concentrations, having irregular cycles or more severe menstrual pain was not associated with urinary 8-OHdG concentration.
Methyl and Total Mercury in Different Media and Associated Fluxes in a Watershed Forest, Southwest China
Authors: Du Hongxia , Ma Ming , Sun Tao , An Siwei , Igarashi Yasuo , Wang Dingyong
Published: November 2018

Mercury (Hg) deposition in the forest ecosystem is a significant source of input for methyl Hg (MeHg) and total Hg (THg) to the subtropical forest field and downstream aquatic systems. Wet deposition, litterfall, runoff, and fluxes with forest soil percolate of MeHg and THg were sampled for two years in a watershed forest of southwest China. Results showed that the depositions of THg and MeHg through litterfall and throughfall were 86 µg m−2 yr−1 and 0.8 µg m−2 yr−1 respectively, with litterfall acting as a predominant route for the input of both THg and MeHg. The estimated fluxes of THg and MeHg in the throughfall and litterfall were 3 and 4 times greater than those in the precipitation. Methylmercury in the decomposed litter migrates during its erosion by surface runoff and the concentrations of MeHg were quite consistent with that in the surface runoff. Methylmercury mainly accumulated in the lower layer of the litter and upper layer of the soil (Oi), and its transfer through the soil cross-section was delayed. THg retention was not consistent with MeHg, probably with lower soil layers (Oe and Oa) storing and enriching THg in the forest ecosystem. The forest floor of the lower soil is an effective sink for THg but not for MeHg. Methylmercury accumulated in decomposing litter and upper soil layer might transfer with soil percolate, possessing potential ecological risks for residents living around the downstream aquatic systems.
The Risk of Mortality among Psoriatic Patients with Varying Severity: A Nationwide Population-Based Cohort Study in Taiwan
Authors: Dai Ying-Xiu , Hsu Ming-Chun , Hu Hsiao-Yun , Chang Yun-Ting , Chen Tzeng-Ji , Li Chung-Pin , Wu Chen-Yi
Published: November 2018

Background: Previous studies showed conflicting results regarding the mortality risk in psoriasis patients with respect to disease severity and presence of psoriatic arthritis. This study aimed to determine the mortality risk in patients with mild and severe psoriasis and patients with psoriatic arthritis (PsA). Methods: A nationwide population-based cohort study was conducted based on data from the Taiwan National Health Insurance Research Database between 2002 and 2012. Incident psoriasis subjects were classified into two groups: psoriasis without arthritis and psoriasis with arthritis. Patients who had received systemic therapy and/or phototherapy were classified as having severe psoriasis; otherwise, patients were classified as having mild psoriasis. Control subjects without psoriasis were selected to match each psoriasis patient from the database within the same observational period. Cox proportional hazards analysis was used to compare the hazard ratio (HR) of time to death. Results: A total of 106,701 patients with psoriasis were included in this study. After controlling for demographics and comorbidities, psoriasis patients had a higher mortality risk compared with the control group (HR 1.41; 95% confidence interval (CI) 1.36 to 1.46). Compared with psoriasis alone, the mortality risk was not increased for PsA (HR = 1.01; 95% CI 0.93 to 1.10). Besides, severe psoriasis did not increase mortality risk compared with mild psoriasis (HR = 1.0; 95% CI 0.95 to 1.06). Conclusions: Patients with psoriasis had a higher mortality risk compared with control subjects, whereas psoriasis severity and presence of PsA had no impact on mortality risk in psoriasis patients.
Soft Tissue Contour Impression with Analogic or Digital Work Flow: A Case Report
Authors: Canullo Luigi , Di Domenico Andrea , Marinotti Fabio , Menini Maria , Pesce Paolo
Published: November 2018

Purpose: Transferring precise information to the dental laboratory is one of the key factors to achieving clinical success. The aim of the present study was to describe classical and digital work-flows used to rehabilitate an implant with a convergent collar in the aesthetic zone following the BOPT (biologically oriented preparation technique) approach and to report the three years follow-up outcomes of two patients rehabilitated following such procedures. Materials and methods: Two central incisors of two different patients were rehabilitated with a tissue-level implant with a convergent collar and, after a provisional and healing phase, one implant was “impressed” using a classical workflow and one using a digital one. The primary outcome measured was the mean bone loss. An intraoral radiograph was taken at crown delivery and at the three years follow-up visit. Secondary Esthetic outcomes pre-op and post-op were evaluated using the PES (pink esthetic score). Results: At the three years follow-up visit, radiographic analysis showed no signs of bone resorption. For the analogic procedure, the pre-and post-op PES scores were 8, whereas for the digital procedure the pre-op PES score was 4 and a post-op score of 9 was obtained. Conclusions: classical and digital work flows succeeded in giving precise and complete anatomical information of implant position, including the soft tissue contour. Minimum bone loss and an esthetic success were obtained in both procedures.
Assessment of Patient and Occupational Safety Culture in Hospitals: Development of a Questionnaire with Comparable Dimensions and Results of a Feasibility Study in a German University Hospital
Authors: Wagner Anke , Michaelis Martina , Luntz Luntz , Wittich Andrea , Schrappe Matthias , Lessing Constanze , Rieger Monika A.
Published: November 2018

(1) Background: Both patient and occupational safety cultures should be considered when promoting safety culture. To our knowledge, there are no studies that capture patient safety culture (PSC) and occupational safety culture (OSC) in hospitals while using a common questionnaire. The aim of this feasibility study in a German university hospital was to develop a questionnaire to assess both issues analogously. In addition to feasibility outcomes, we report results of PSC-OSC comparisons. (2) Methods: To assess PSC, we used the existing Hospital Survey on Patient Safety Culture (HSPSC) questionnaire. Developing new OSC “twin items” for certain parts of the HSPSC was supported by a previous literature review. Additionally, we developed multiple choice questions to examine knowledge and competencies regarding specific PS/OS aspects. (3) Results: Developing and implementing a combined PSC and OSC assessment instrument was feasible. The overall response rate was 33% (407 nurses, 140 physicians). In general, the statistical reliability of almost all scales was sufficient. Positive PSC perceptions (agreement rates 46–87%) were found in 16 out of 18 scales. Of the four twin scales, the PSC values were significantly better. Individual PS- and OS-related knowledge and competencies were lower than expected. (4) Conclusion: The comparative investigation of patient and occupational safety in a large hospital is a promising approach and can be recommended for further studies. We used our experiences that are presented here in an ongoing bicentric study on the associations between working conditions, occupational safety culture, patient safety culture, and patient safety outcomes (WorkSafeMed).
Whole Genome Sequencing of a Vietnamese Family from a Dioxin Contamination Hotspot Reveals Novel Variants in the Son with Undiagnosed Intellectual Disability
Authors: Nguyen Dang Ton , Nguyen Hai Ha , Nguyen Thuy Duong , Nguyen Thi Thanh Hoa , Nakano Kaoru , Maejima Kazuhiro , Sasaki-Oku Aya , Nguyen Van Ba , Nguyen Duy Bac , Le Bach Quang , Wong Jing Hao , Tsunoda Tatsuhiko , Nakagawa Hidewaki , Fujimoto Akihiro , Nong Van Hai
Published: November 2018

Although it has been a half-century since dioxin-contaminated herbicides were used to defoliate the landscape during the Vietnam War, dioxin contamination “hotspots” still remain in Vietnam. Environmental and health impacts of these hotspots need to be evaluated. Intellectual disability (ID) is one of the diseases found in the children of people exposed to the herbicides. This study aims to identify genetic alterations of a patient whose family lived in a dioxin hotspot. The patient’s father had a highly elevated dioxin concentration. He was affected with undiagnosed moderate ID. To analyze de novo mutations and genetic variations, and to identify causal gene(s) for ID, we performed whole genome sequencing (WGS) of the proband and his parents. Two de novo missense mutations were detected, each one in ETS2 and ZNF408 genes, respectively. Compound heterozygosity was identified in CENPF and TTN genes. Existing knowledge on the genes and bioinformatics analyses suggest that EST2, ZNF408, and CENPF might be promising candidates for ID causative genes.
A Rapid Method for the Detection of Sarcosine Using SPIONs/Au/CS/SOX/NPs for Prostate Cancer Sensing
Authors: Uhlirova Dagmar , Stankova Martina , Docekalova Michaela , Hosnedlova Bozena , Kepinska Marta , Ruttkay-Nedecky Branislav , Ruzicka Josef , Fernandez Carlos , Milnerowicz Halina , Kizek Rene
Published: November 2018

Background: Sarcosine is an amino acid that is formed by methylation of glycine and is present in trace amounts in the body. Increased sarcosine concentrations in blood plasma and urine are manifested in sarcosinemia and in some other diseases such as prostate cancer. For this purpose, sarcosine detection using the nanomedicine approach was proposed. In this study, we have prepared superparamagnetic iron oxide nanoparticles (SPIONs) with different modified surface area. Nanoparticles (NPs) were modified by chitosan (CS), and sarcosine oxidase (SOX). SPIONs without any modification were taken as controls. Methods and Results: The obtained NPs were characterized by physicochemical methods. The size of the NPs determined by the dynamic light scattering method was as follows: SPIONs/Au/NPs (100–300 nm), SPIONs/Au/CS/NPs (300–700 nm), and SPIONs/Au/CS/SOX/NPs (600–1500 nm). The amount of CS deposited on the NP surface was found to be 48 mg/mL for SPIONs/Au/CS/NPs and 39 mg/mL for SPIONs/Au/CS/SOX/NPs, and repeatability varied around 10%. Pseudo-peroxidase activity of NPs was verified using sarcosine, horseradish peroxidase (HRP) and 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. For TMB, all NPs tested evinced substantial pseudo-peroxidase activity at 650 nm. The concentration of SPIONs/Au/CS/SOX/NPs in the reaction mixture was optimized to 0–40 mg/mL. Trinder reaction for sarcosine detection was set up at 510 nm at an optimal reaction temperature of 37 °C and pH 8.0. The course of the reaction was linear for 150 min. The smallest amount of NPs that was able to detect sarcosine was 0.2 mg/well (200 µL of total volume) with the linear dependence y = 0.0011x − 0.0001 and the correlation coefficient r = 0.9992, relative standard deviation (RSD) 6.35%, limit of detection (LOD) 5 µM. The suggested method was further validated for artificial urine analysis (r = 0.99, RSD 21.35%, LOD 18 µM). The calculation between the detected and applied concentrations showed a high correlation coefficient (r = 0.99). NPs were tested for toxicity and no significant growth inhibition was observed in any model system (S. cerevisiae, S. aureus, E. coli). The hemolytic activity of the prepared NPs was similar to that of the phosphate buffered saline (PBS) control. The reaction system was further tested on real urine specimens. Conclusion: The proposed detection system allows the analysis of sarcosine at micromolar concentrations and to monitor changes in its levels as a potential prostate cancer marker. The whole system is suitable for low-cost miniaturization and point-of-care testing technology and diagnostic systems. This system is simple, inexpensive, and convenient for screening tests and telemedicine applications.
Engineered Mesenchymal Stem Cells Expressing Stromal Cell-derived Factor-1 Improve Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats
Authors: Jeon Seung Hwan , Zhu Guan Qun , Bae Woong Jin , Choi Sae Woong , Jeong Hyun Cheol , Cho Hyuk Jin , Ha U-Syn , Hong Sung-Hoo , Lee Ji Youl , Kwon Eun Bi , Kim Hyo-Jin , Lee Soon Min , Kim Hey-Yon , Kim Sae Woong
Published: November 2018

Effective therapies for erectile dysfunction (ED) associated with diabetes mellitus (DM) are needed. In this study, the effects of stromal cell-derived factor-1 (SDF-1)-expressing engineered mesenchymal stem cells (SDF-1 eMSCs) and the relevant mechanisms in the corpus cavernosum of a streptozotocin (STZ)-induced DM ED rat model were evaluated. In a randomized controlled trial, Sprague–Dawley (SD) rats (n = 48) were divided into four groups (n = 12/group): Normal (control), DM ED (diabetes induced by STZ), DM ED + BM-MSC (treated with bone marrow [BM]-derived MSCs), and DM ED + SDF-1 eMSC (treated with SDF-1-expressing BM-MSCs). After four weeks, intracavernosal pressure (ICP), an indicator of erectile function, was 0.75 ± 0.07 in the normal group, 0.27 ± 0.08 in the DM ED group, 0.42 ± 0.11 in the DM ED + BM-MSC group, and 0.58 ± 0.11 in the DM ED + SDF-1 eMSC group. BM-MSCs, especially SDF-1 eMSCs, improved ED (p < 0.05). SDF-1 eMSC treatment improved the smooth muscle content in the corpus cavernosum (p < 0.05). As SDF-1 expression increased, ED recovery improved. In the SDF-1 eMSC group, levels of neuronal nitric oxide synthase (nNOS) and phosphorylated endothelial NOS (p-eNOS) were higher than those in other groups (p < 0.05). In addition, high stromal cell-derived factor-1 (SDF-1) expression was associated with increased vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in DM ED rats (p < 0.05). Higher levels of phosphorylated protein kinase B (p-AKT)/protein kinase B (AKT) (p < 0.05) and B-cell lymphoma-2 (Bcl-2) and lower levels of the apoptosis factors Bcl2-associated x (Bax) and caspase-3 were observed in the MSC-treated group than in the DM ED group (p < 0.05). SDF-1 eMSCs showed beneficial effects on recovery from erectile function.
Underground Space Utilization in the Urban Land-Use Planning of Casablanca (Morocco)
Authors: Zerhouny Mariama , Fadil Abdelhamid , Hakdaoui Mustapha
Published: November 2018

With the rapid rate of population growth and economic development, cities face enormous challenges that require both optimal and integrated solutions to meet the needs of growth and to protect the environment and sustainable development. These urban dynamics, which change over time, extend not only horizontally and upward, but also downward. Thus, underground space has been utilized increasingly to relieve the urban surface and to ensure the exploitation of underground resources. The purpose of this study is to evaluate the possibilities of using this space in Casablanca as part of urban land-use planning and, consequently, to suggest an integrated model of exploitation of this space that is adapted to the specificities of the study area. Thus, an analysis of the use of underground spaces in a set of European cities has been performed. The study of the characteristics of this space in Casablanca has been realized according to the levels of geology and hydrogeology and two underground infrastructure projects. This work has led to the implementation of a prototype model named “Sub-Urban Information Modeling”. The model’s objective is to gather all the data and knowledge related to the relevant underground space in an integrated platform that can be shared and updated in order to facilitate the understanding of this environment and its interaction with the surface and to ensure the rational and efficient use of its resources.
Development of Laser-Based Powder Bed Fusion Process Parameters and Scanning Strategy for New Metal Alloy Grades: A Holistic Method Formulation
Authors: Bassoli Elena , Sola Antonella , Celesti Mattia , Calcagnile Sandro , Cavallini Carlo
Published: November 2018

In spite of the fast growth of laser-based powder bed fusion (L-PBF) processes as a part of everyday industrial practice, achieving consistent production is hampered by the scarce repeatability of performance that is often encountered across different additive manufacturing (AM) machines. In addition, the development of novel feedstock materials, which is fundamental to the future growth of AM, is limited by the absence of established methodologies for their successful exploitation. This paper proposes a structured procedure with a complete test plan, which defines step-by-step the standardized actions that should be taken to optimize the processing parameters and scanning strategy in L-PBF of new alloy grades. The method is holistic, since it considers all the laser/material interactions in different local geometries of the build, and suggests, for each possible interaction, a specific geometry for test specimens, standard energy parameters to be analyzed through a design of experiment, and measurable key performance indicators. The proposed procedure therefore represents a sound and robust aid to the development of novel alloy grades for L-PBF and to the definition of the most appropriate processing conditions for them, independent of the specific AM machine applied.
Injection Molding of Thermoplastic Cellulose Esters and Their Compatibility with Poly(Lactic Acid) and Polyethylene
Authors: Willberg-Keyriläinen Pia , Orelma Hannes , Ropponen Jarmo
Published: November 2018

Interest in biobased polymers from renewable resources has grown in recent years due to environmental concerns, but they still have a minimal fraction of the total global market. In this study, the injection molding of thermoplastic cellulose octanate (cellulose C8) and cellulose palmitate (cellulose C16) were studied. The mechanical properties of injection-molded test specimens were analyzed by using tensile testing, and the internal structure of injection-molded objects was studied by using a field emission scanning electron microscopy (FE-SEM). We showed that thermoplastic cellulose C8 and cellulose C16 were completely processable without the addition of a plasticizer, which is very unusual in the case of cellulose esters. The compatibility of cellulose esters with poly(lactic acid) (PLA) and biopolyethylene (bio-PE) was also tested. By compounding the cellulose esters with PLA, the elongation of PLA-based blends could be improved and the density could be reduced. The tested thermoplastic cellulose materials were fully biobased, and have good future potential to be used in injection molding applications.
The Use of the FEM to Identify the Optimal Groove Dimensions Ensuring the Least Stressed Connection between a Zirconia Coping and Veneering Ceramic
Authors: Śmielak Beata , Klimek Leszek , Świniarski Jacek
Published: November 2018

Background: To examine the influence of coping notches with varying groove widths and depths on the quality of the connection with ceramic. Methods: Ten rectangular sintered zirconia (3Y-TZP) samples were etched with a neodymium-yag laser Nd:YAG. Then, a profilometer was used to test the depths and spacing of the grooves. A notch profile was used to design the shapes and spacing of the grooves based on a finite element method (FEM) simulating zirconia. The following situations were simulated: an increase in groove width from 100% to 180% and depth from 40% and 80%; and a 40% depth and width. Results: An increase of 10% in the baseline width caused an insignificant reduction of the strain in the connection. A further increase in this dimension led to a 50% increase in strain with a 40% increase in width. An increase in the groove depth by 40% reduced the strain level by 13%, while an increase in the groove depth by 80% reduced the strain level by 22%. Simultaneous deepening and widening of the groove by 40% had no significant impact on the strain level. Conclusion: Maintaining the width of the groove bottom while increasing the depth offers fewer advantages than deepening and narrowing the groove bottom.
Using the Outlier Detection Task to Evaluate Distributional Semantic Models
Authors: Gamallo Pablo
Published: November 2018

In this article, we define the outlier detection task and use it to compare neural-based word embeddings with transparent count-based distributional representations. Using the English Wikipedia as a text source to train the models, we observed that embeddings outperform count-based representations when their contexts are made up of bag-of-words. However, there are no sharp differences between the two models if the word contexts are defined as syntactic dependencies. In general, syntax-based models tend to perform better than those based on bag-of-words for this specific task. Similar experiments were carried out for Portuguese with similar results. The test datasets we have created for the outlier detection task in English and Portuguese are freely available.
Ceratinadins E and F, New Bromotyrosine Alkaloids from an Okinawan Marine Sponge Pseudoceratina sp.
Authors: Kurimoto Shin-ichiro , Ohno Taito , Hokari Rei , Ishiyama Aki , Iwatsuki Masato , Ōmura Satoshi , Kobayashi Jun’ichi , Kubota Takaaki
Published: November 2018

Two new bromotyrosine alkaloids, ceratinadins E (1) and F (2), were isolated from an Okinawan marine sponge Pseudoceratina sp. as well as a known bromotyrosine alkaloid, psammaplysin F (3). The gross structures of 1 and 2 were elucidated on the basis of spectroscopic data. The absolute configurations of 1 and 2 were assigned by comparison of the NMR and ECD data with those of a known related bromotyrosine alkaloid, psammaplysin A (4). Ceratinadins E (1) and F (2) are new bromotyrosine alkaloids possessing an 8,10-dibromo-9-methoxy-1,6-dioxa-2-azaspiro[4.6]undeca-2,7,9-trien-4-ol unit with two or three 11-N-methylmoloka’iamine units connected by carbonyl groups, respectively. Ceratinadin E (1) exhibited antimalarial activities against a drug-resistant and a drug-sensitive strains of Plasmodium falciparum (K1 and FCR3 strains, respectively).
Preventive Effect of Lactobacillus fermentum CQPC03 on Activated Carbon-Induced Constipation in ICR Mice
Authors: Zhang Jing , Chen Benshou , Liu Baosi , Zhou Xianrong , Mu Jianfei , Wang Qiang , Zhao Xin , Yang Zhennai
Published: November 2018

Background and objectives: Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated Lactobacillus was investigated. Materials and Methods: The 16S rDNA technology was used for microbial identification. A mouse constipation model was established using activated carbon. After intragastric administration of Lactobacillus (109 CFU/mL), the mice were dissected to prepare pathological sections of the small intestine. Serum indicators were detected using kits, and the expression of small intestine-related mRNAs was detected by qPCR assay. Results: One strain of Lactobacillus was identified and named Lactobacillus fermentum CQPC03 (LF-CQPC03). Body weight and activated carbon propulsion rate were all higher in mice intragastrically administered with LF-CQPC03 compared with the control group, while the time to the first black stool in treated mice was lower than that in the control group. Serum assays showed that gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels were significantly higher in the LF-CQPC03-treated mice than in the control group, while somatostatin (SS) levels were significantly lower than in the control mice. Mouse small intestine tissue showed that c-Kit, stem cell factor (SCF), and glial cell-derived neurotrophic factor (GDNF) mRNA expression levels were significantly higher in the LF-CQPC03 treated mice than in control mice, while transient receptor potential cation channel subfamily V member 1 (TRPV1) and inducible nitric oxide synthase (iNOS) expression levels were significantly lower in the LF-CQPC03 treated mice than in control mice. Conclusions: There is a better effect with high-dose LF-CQPC03, compared to the lower dose (LF-CQPC03-L), showing good probiotic potential, as well as development and application value.
Similarities in Blood Mononuclear Cell Membrane Phospholipid Profiles during Malignancy
Authors: Hakobyan Gohar , Davtyan Hasmik , Harutyunyan Kristine , Alexanyan Knarik , Amirkhanyan Yelizaveta , Gharibyan Anna L. , Asatryan Liana , Tadevosyan Yuri
Published: November 2018

Phospholipids (PLs), key elements of cellular membranes, are regulated reciprocally with membrane proteins and can act as sensors for alterations in physiological or pathological states of cells including initiation and development of cancer. On the other hand, peripheral blood mononuclear cells (MNCs) play an important role in antitumor immune response by reacting to cancerous modifications in distant organs. In the current study, we tested the hypothesis that tumor initiation and development are reflected in the alteration pattern of the MNC PL component. We analyzed MNC membrane PL fractions in samples from healthy individuals and from patients with diverse types of cancers to reveal possible alterations induced by malignancy. Compared to healthy controls, the cancer samples demonstrated shifts in several membrane PL profiles. In particular, when analyzing cancer data pooled together, there were significantly higher levels in lysophosphatidylcholine, phosphatidylcholine, and phosphatidylethanolamine fractions, and significantly lower quantities in phosphatidylinositol, phosphatidylserine, and phosphatidic acid fractions in cancer samples compared to controls. The levels of sphingomyelins and diphosphatidylglycerols were relatively unaffected. Most of the differences in PLs were sustained during the analysis of individual cancers such as breast cancer and chronic lymphocytic leukemia. Our findings suggest the presence of a common pattern of changes in MNC PLs during malignancy.
Steering Algorithm for a Flexible Microrobot to Enhance Guidewire Control in a Coronary Angioplasty Application
Authors: Kafash Hoshiar Ali , Jeon Sungwoong , Kim Kangho , Lee Seungmin , Kim Jin-young , Choi Hongsoo
Published: November 2018

Magnetically driven microrobots have been widely studied for various biomedical applications in the past decade. An important application of these biomedical microrobots is heart disease treatment. In intravascular treatments, a particular challenge is the submillimeter-sized guidewire steering; this requires a new microrobotic approach. In this study, a flexible microrobot was fabricated by the replica molding method, which consists of three parts: (1) a flexible polydimethylsiloxane (PDMS) body, (2) two permanent magnets, and (3) a micro-spring connector. A mathematical model was developed to describe the relationship between the magnetic field and the deformation. A system identification approach and an algorithm were proposed for steering. The microrobot was fabricated, and the models for steering were experimentally validated under a magnetic field intensity of 15 mT. Limitations to control were identified, and the microrobot was steered in an arbitrary path using the proposed model. Furthermore, the flexible microrobot was steered using the guidewire within a three-dimensional (3D) transparent phantom of the right coronary artery filled with water, to show the potential application in a realistic environment. The flexible microrobot presented here showed promising results for enhancing guidewire steering in percutaneous coronary intervention (PCI).
Geochemistry and Petrogenesis of Mesoproterozoic Dykes of the Irkutsk Promontory, Southern Part of the Siberian Craton
Authors: Donskaya T. V. , Gladkochub D. P. , Ernst R. E. , Pisarevsky S. A. , Mazukabzov A. M. , Demonterova E. I.
Published: November 2018

We present new geochemical and Nd isotopic data on two Mesoproterozoic Listvyanka (1350 ± 6 Ma) and Goloustnaya (1338 ± 3 Ma) mafic dyke swarms located in the Irkutsk Promontory of the southern part of the Siberian craton. Listvyanka dykes are sub-vertical with NNE trend, while Goloustnaya dykes are characterized by prevailing W trend. Listvyanka and Goloustnaya dykes are composed of medium to coarse grained dolerites. All dolerites correspond to sub-alkaline tholeiitic basalts according to their major-element compositions with lower to moderate mg#, varying from 36 to 54. The trace and rare earth element abundances in Listvyanka and Goloustnaya dolerites are generally close to basalts of the oceanic island basalts (OIB) type. The Listvyanka dolerites demonstrate slightly positive εNd(t) values varying from +1.1 to +1.5, while the Goloustnaya dolerites are characterized by lower εNd(t) values ranging from −0.9 to +0.1. Geochemical and isotopic affinities of the Listvyanka dolerites suggest their enrichment by a mantle plume related source. For the Goloustnaya dolerites, we assume also some additional lithospheric input to their mantle plume-related source. The emplacement of both studied dolerites took place in intracontinental extensional setting, caused by a single rising mantle plume. Listvyanka and Goloustnaya dolerites are coeval to several mafic magmatic events in northern Laurentia and likely represent part of the Mesoproterozoic plumbing system of a Siberian–Laurentian Large Igneous Province.
Selenium, Selenoproteins, and Female Reproduction: A Review
Authors: Qazi Izhar Hyder , Angel Christiana , Yang Haoxuan , Pan Bo , Zoidis Evangelos , Zeng Chang-Jun , Han Hongbing , Zhou Guang-Bin
Published: November 2018

Selenium (Se) is an essential micronutrient that has several important functions in animal and human health. The biological functions of Se are carried out by selenoproteins (encoded by twenty-five genes in human and twenty-four in mice), which are reportedly present in all three domains of life. As a component of selenoproteins, Se has structural and enzymatic functions; in the latter context it is best recognized for its catalytic and antioxidant activities. In this review, we highlight the biological functions of Se and selenoproteins followed by an elaborated review of the relationship between Se and female reproductive function. Data pertaining to Se status and female fertility and reproduction are sparse, with most such studies focusing on the role of Se in pregnancy. Only recently has some light been shed on its potential role in ovarian physiology. The exact underlying molecular and biochemical mechanisms through which Se or selenoproteins modulate female reproduction are largely unknown; their role in human pregnancy and related complications is not yet sufficiently understood. Properly powered, randomized, controlled trials (intervention vs. control) in populations of relatively low Se status will be essential to clarify their role. In the meantime, studies elucidating the potential effect of Se supplementation and selenoproteins (i.e., GPX1, SELENOP, and SELENOS) in ovarian function and overall female reproductive efficiency would be of great value.
Identification of Ubiquinones in Honey: A New View on Their Potential Contribution to Honey’s Antioxidant State
Authors: Brudzynski Katrina , Maldonado-Alvarez Liset
Published: November 2018

Honey is composed of macromolecules arranged into multicomponent colloidal particles dispersed in a supersaturated sugar solution. The core part of colloidal particles in honey is made up of high-molecular weight protein-polyphenol complexes. We designed a multi-step extraction process to gain better insight into the phenolic compounds strongly bound to proteins in honey. Honeys were sequentially extracted by solvents of reduced polarities and the extraction process was monitored by LC-ESI-MS/MS. Unexpectedly, the results revealed ubiquinone-like compounds that partitioned to both, soluble supernatants and protein-bound insoluble residues from which they were released after the pronase-digestion of proteins. The accurate mass measurement and MS/MS fragmentation patterns using UPHLC-MS/MS coupled to quadrupole orbitrap confirmed their identification as ubiquinones. Distribution of ubiquinone-bound proteins was further investigated by the fractionation of honey protein-polyphenol complexes by size-exclusion chromatography followed by LC-ESI-MS analysis. Mass spectra revealed the presence of ubiquinones (UQs) in fractions of high polyphenol to protein ratio. The dominant mass peaks observed in these fractions were identified as UQ-3, UQ-5, and UQ-7. Since the quinone group of UQs is involved in redox reaction, we discuss the possibility that UQs may contribute to the antioxidant/proxidant activity of these complexes.
Antimicrobial Activity of Divaricatic Acid Isolated from the Lichen Evernia mesomorpha against Methicillin-Resistant Staphylococcus aureus
Authors: Oh Jong Min , Kim Yi Jeong , Gang Hyo-Seung , Han Jin , Ha Hyung-Ho , Kim Hoon
Published: November 2018

One hundred and seventy seven acetone extracts of lichen and 258 ethyl acetate extracts of cultured lichen-forming fungi (LFF) were screened for antimicrobial activity against Staphylococcus aureus and Enterococcus faecium using a disk diffusion method. Divaricatic acid was isolated from Evernia mesomorpha and identified by LC-MS, 1H-, 13C- and DEPT-NMR. Purified divaricatic acid was effective against Gram + bacteria, such as Bacillus subtilis, Staphylococcus epidermidis, Streptococcus mutans, and Enterococcus faecium, with the minimum inhibitory concentration (MIC) values ranging from 7.0 to 64.0 μg/mL, whereas vancomycin was effective in the MICs ranging from 0.78 to 25.0 μg/mL. Interestingly, the antibacterial activity of divaricatic acid was higher than vancomycin against S. epidermidis and E. faecium, and divaricatic acid was active against Candida albicans. In addition, divaricatic acid was active as vancomycin against S. aureus (3A048; an MRSA). These results suggested that divaricatic acid is a potential antimicrobial agent for the treatment of MRSA infections.
Combining Dietary Sulfur Amino Acid Restriction with Polyunsaturated Fatty Acid Intake in Humans: A Randomized Controlled Pilot Trial
Authors: Olsen Thomas , Øvrebø Bente , Turner Cheryl , Bastani Nasser , Refsum Helga , Vinknes Kathrine
Published: November 2018

Dietary and plasma total cysteine (tCys) have been associated with adiposity, possibly through interaction with stearoyl–CoA desaturase (SCD), which is an enzyme that is involved in fatty acid and energy metabolism. We evaluated the effect of a dietary intervention with low cysteine and methionine and high polyunsaturated fatty acids (PUFAs) on plasma and urinary sulfur amino acids and SCD activity indices. Fourteen normal-weight healthy subjects were randomized to a seven-day diet low in cysteine and methionine and high in PUFAs (Cys/Metlow + PUFA), or high in saturated fatty acids (SFA), cysteine, and methionine (Cys/Methigh + SFA). Compared with the Cys/Methigh + SFA group, plasma methionine and cystathionine decreased (p-values < 0.05), whereas cystine tended to increase (p = 0.06) in the Cys/Metlow + PUFA group. Plasma total cysteine (tCys) was not significantly different between the groups. Urinary cysteine and taurine decreased in the Cys/Metlow + PUFA group compared with the Cys/Methigh + SFA group (p-values < 0.05). Plasma SCD-activity indices were not different between the groups, but the change in cystine correlated with the SCD-16 index in the Cys/Metlow + PUFA group. A diet low in methionine and cysteine decreased plasma methionine and urinary cysteine and taurine. Plasma tCys was unchanged, suggesting that compensatory mechanisms are activated during methionine and cysteine restriction to maintain plasma tCys.
Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity
Authors: Vencioneck Dutra Jean Carlos , Moisés Ferreira Jean , Costalonga Pereira Paula Roberta , Ben-Hur de Oliveira Judá , Vitorino Gervásio Suiany , Bernardes Xavier Mirieli , Mantovanelli da Mota Mainã , Luz Anny Carolyne da , Rodrigues Pretti Irany , Seibert França Hildegardo , Masrouah Jamal Claudia , Pimentel Batitucci Maria do Carmo
Published: November 2018

Cereus jamacaru D.C. (mandacaru) is a cactus used as food and in the traditional medicine. In the present study, hydroalcoholic extract of C. jamacaru was evaluated for its chemical composition, antioxidant activity, cytotoxic and anti-cytotoxic effects in human lymphocytes and sarcoma 180 cells in vitro by MTT assay and antitumoral, mutagenic and cytotoxic effects on mice sarcoma-induced in vivo. Phytochemical characterization showed positive reactions for coumarin, flavanol and tyramine and total flavonoid content of 0.51 µg/mL. C. jamacaru showed antioxidant activity following DPPH (EC50 = 427.74 µg/mL), ABTS (EC50 = 270.57 µg/mL) and Fe2+ chelating ions assays (EC50 = 41.18 µg/mL). C. jamacaru induced significant decrease of sarcoma 180 viability at 24 h and 48 h of treatment, did not induce cytotoxicity in human lymphocytes and inhibits the cytotoxicity of cisplatin in vitro. Following in vivo assays, C. jamacaru promoted tumor reduction (86.07% of tumor inhibition), without inducing mutagenic or cytotoxic damage on mice blood cells. We propose that phenolic and alkaloid compounds in the extract are related to antioxidant activity, increasing its ability in metal chelating activity and promoting anti-cytotoxic activity against cisplatin, as well as these compounds may act on the cell cycle of the tumor cells in vitro and in vivo, leading to anticancer effects and tumor reduction.
Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length
Authors: Annenkov Vadim V. , Krishnan Uma Maheswari , Pal’shin Viktor A. , Zelinskiy Stanislav N. , Kandasamy Gayathri , Danilovtseva Elena N.
Published: November 2018

Amine containing polymers are extensively studied as special carriers for short-chain RNA (13–25 nucleotides), which are applied as gene silencing agents in gene therapy of various diseases including cancer. Elaboration of the oligonucleotide carriers requires knowledge about peculiarities of the oligonucleotide–polymeric amine interaction. The critical length of the interacting chains is an important parameter which allows us to design sophisticated constructions containing oligonucleotide binding segments, solubilizing, protective and aiming parts. We studied interactions of (TCAG)n, n = 1–6 DNA oligonucleotides with polyethylenimine and poly(N-(3-((3-(dimethylamino)propyl)(methyl)amino)propyl)-N-methylacrylamide). The critical length for oligonucleotides in interaction with polymeric amines is 8–12 units and complexation at these length can be accompanied by “all-or-nothing” effects. New dimethylacrylamide based polymers with grafted polyamine chains were obtained and studied in complexation with DNA and RNA oligonucleotides. The most effective interaction and transfection activity into A549 cancer cells and silencing efficiency against vascular endothelial growth factor (VEGF) was found for a sample with average number of nitrogens in polyamine chain equal to 27, i.e., for a sample in which all grafted chains are longer than the critical length for polymeric amine–oligonucleotide complexation.