bluuebunny/crossref_metadata_2025 huggingface dataset:
Aeroacoustic Coupling in Rectangular Deep Cavities: Passive Control and Flow Dynamics
Authors: Jabado Abdul Hamid , El Hassan Mouhammad , Hammoud Ali , Sakout Anas , Assoum Hassan H.
Published: August 2024

Deep cavity configurations are common in various industrial applications, including automotive windows, sunroofs, and many other applications in aerospace engineering. Flows over such a geometry can result in aeroacoustic coupling between the cavity shear layer oscillations and the surrounding acoustic modes. This phenomenon can result in a resonance that can lead to significant noise and may cause damage to mechanical structures. Flow control methods are usually used to reduce or eliminate the aeroacoustic resonance. An experimental set up was developed to study the effectiveness of both a cylinder and a profiled cylinder positioned upstream from the cavity in reducing the flow resonance. The cavity flow and the acoustic signals were obtained using particle image velocimetry (PIV) and unsteady pressure sensors, respectively. A decrease of up to 36 dB was obtained in the sound pressure levels (SPL) using the passive control methods. The profiled cylinder showed a similar efficacy in reducing the resonance despite the absence of a high-frequency forcing. Time-space cross-correlation maps along the cavity shear layer showed the suppression of the feedback mechanism for both control methods. A snapshot proper orthogonal decomposition (POD) showed interesting differences between the cylinder and profiled cylinder control methods in terms of kinetic energy content and the vortex dynamics behavior. Furthermore, the interaction of the wake of the control device with the cavity shear layer and its impact on the aeroacoustic coupling was investigated using the POD analysis.
Comprehensive Analysis of Allulose Production: A Review and Update
Authors: Wang Lei , Cui Yun , Lu Yujie , Zhao Zongpei
Published: August 2024

Advancements in D-allulose production have seen significant strides in recent years, focusing on enzymatic conversion methods. Key developments include traditional immobilization techniques, the discovery of novel enzymes, directed evolution studies, and biosynthesis through metabolic pathway modification. Enzymatic conversion, particularly utilizing D-allulose 3-epimerase, remains fundamental for industrial-scale production. Innovative immobilization strategies, such as functionalized nano-beads and magnetic MOF nanoparticles, have significantly enhanced enzyme stability and reusability. Directed evolution has led to improved enzyme thermostability and catalytic efficiency, while synthetic biology methods, including phosphorylation-driven and thermodynamics-driven pathways, have optimized production processes. High-throughput screening methods have been crucial in identifying and refining enzyme variants for industrial applications. Collectively, these advancements not only enhance production efficiency and cost-effectiveness but also adhere to sustainable and economically viable manufacturing practices. The past five years have witnessed critical developments with significant potential impact on the commercial viability and global demand for allulose.
Health Hazard Associated with the Presence of Clostridium Bacteria in Food Products
Authors: Bilska Agnieszka , Wochna Krystian , Habiera Małgorzata , Serwańska-Leja Katarzyna
Published: August 2024

Clostridium bacteria were already known to Hippocrates many years before Christ. The name of the Clostridium species is owed to the Polish microbiologist, Adam Prażmowski. It is now known that these Clostridium bacteria are widespread in the natural environment, and their presence in food products is a threat to human health and life. According to European Food Safety Authority (EFSA) reports, every year, there are poisonings or deaths due to ingestion of bacterial toxins, including those of the Clostridium spp. The strengthening of consumer health awareness has increased interest in consuming products with minimal processing in recent years, which has led to a need to develop new techniques to ensure the safety of microbiological food, including elimination of bacteria from the Clostridium genera. On the other hand, the high biochemical activity of Clostridium bacteria allows them to be used in the chemical, pharmaceutical, and medical industries. Awareness of microbiological food safety is very important for our health. Unfortunately, in 2022, an increase in infections with Clostridium bacteria found in food was recorded. Knowledge about food contamination should thus be widely disseminated.
Determination of Cannabinoids in Meat Products and Animal Feeds in Singapore Using Liquid Chromatography–Tandem Mass Spectrometry
Authors: Sin Jia En Valerie , Shen Ping , Huang Lifei , Wu Yuansheng , Chan Sheot Harn
Published: August 2024

There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns on the potential transfer of cannabinoids from hemp-based animal feed to animal products intended for human consumption and its health effects. As such, the detection and quantification of cannabinoids in meat and animal feeds would be desirable for monitoring purposes. In this study, a simple, rapid and sensitive method for the simultaneous quantification of four major cannabinoids (delta-9-tetrahydrocannabinol, cannabidiol, cannabinol and tetrahydrocannabinolic acid) in meat and animal feeds by liquid chromatography–tandem mass spectrometry (LC-MS/MS) was successfully developed and validated. The method was selective and sensitive, achieving limits of detection and quantification for the four cannabinoids from 5 to 7 µg/kg and 15 to 21 µg/kg, respectively. The overall recovery with matrix-matched calibration curves for the cannabinoids ranged from 87–115%. The coefficients of variation were between 2.17–13.38% for intraday precision and 3.67–12.14% for inter-day precision. The method was subsequently applied to monitor cannabinoids in 120 meat and 24 animal feed samples. No cannabinoid was detected, suggesting no imminent food safety concerns arising from the potential incorporation of hemp and by-products in animal feed and nutrition under the promotion of sustainable agricultural practices.
A Comprehensive Review on the Biogenic Amines in Cheeses: Their Origin, Chemical Characteristics, Hazard and Reduction Strategies
Authors: Natrella Giuseppe , Vacca Mirco , Minervini Fabio , Faccia Michele , De Angelis Maria
Published: August 2024

Most of the biogenic amines are naturally found in fermented foods as a consequence of amino acid decarboxylation. Their formation is ascribable to microorganisms (starters, contaminants and autochthonous) present in the food matrix. The concentration of these molecules is important for food security reasons, as they are involved in food poisoning illnesses. The most frequent amines found in foods are histamine, putrescine, cadaverine, tyramine, tryptamine, phenylethylamine, spermine and spermidine. One of the most risk-prone foods are cheeses, mostly ripened ones, which could easily accumulate amines due to their peculiar manufacturing process and ripening. Cheeses represent a pivotal food in our diet, providing for nutrients such as amino acids, calcium, vitamins and others; thus, since they are widely consumed, it is important to evaluate the presence of toxic molecules to avoid consumers’ poisoning. This review aimed to gather general information on the role of biogenic amines, their formation, the health issues and the microorganisms and processes that produce/reduce them, with a focus on their content in different types of cheese (from soft to hard cheeses) and the biotic and abiotic factors that influence their formation or reduction and concentration. Finally, a multivariate analysis was performed on the biogenic amine content, derived from data available in the literature, to obtain more information about the factors influencing their presence in cheeses.
Substitution of Animal Fat and Sodium Nitrite with Hemp Seed Oil: Effect on the Nutritional Value, Sensory Characteristics, and Shelf Life of Fermented Salami
Authors: Papatzimos Georgios , Basdagianni Zoitsa , Kasapidou Eleni
Published: August 2024

Recently, products of plant origin have been utilized to extend the shelf life of meat products. This study examined the impact of hemp seed oil as a replacement for animal fat and sodium nitrite on the nutritional, physicochemical, technological, and sensory traits of fermented salamis. Five treatments were prepared: S0 (100 mg/kg NaNO2), S1 (2% hemp oil and 50 mg/kg NaNO2), S2 (4% hemp oil and 50 mg/kg NaNO2), S3 (2% hemp oil), and S4 (4% hemp oil). The addition of hemp seed oil did not affect proximate composition but improved fatty acid composition and lipid quality nutritional indices. Microbial growth was consistent across all treatments. Active acidity (pH) and water activity (aw) were influenced by hemp seed oil and/or sodium nitrite. Salamis containing only hemp seed oil exhibited lower redness and chroma values during storage. Hemp seed oil led to higher lipid peroxidation, mitigated by sodium nitrite. The addition of hemp seed oil and varying levels of sodium nitrite significantly impacted salami texture. Sensory evaluation showed consumer acceptance of hemp seed oil-enhanced salamis. In conclusion, hemp seed oil can be used as a functional ingredient to improve the nutritional value and healthiness of fermented meat products when combined with reduced sodium nitrite content.
Impact of Drying Process on Grindability and Physicochemical Properties of Celery
Authors: Rudy Stanisław , Dziki Dariusz , Biernacka Beata , Polak Renata , Krzykowski Andrzej , Krajewska Anna , Stanisławczyk Renata , Rudy Mariusz , Żurek Jagoda , Rudzki Grzegorz
Published: August 2024

The objective of this study was to evaluate the impact of various drying methods: freeze drying, vacuum drying, convection drying, and convection-microwave drying at microwave powers of 50 W and 100 W, along with process temperatures (40 °C, 60 °C, and 80 °C), on the drying kinetics, selected physicochemical properties of dried celery stems, and their grindability. The Page model was employed to mathematically describe the drying kinetics across the entire measurement range. Convection-microwave drying significantly reduced the drying time compared to the other methods. The longest drying duration was observed with freeze drying at 40 °C. The product obtained through freeze drying at 40 °C exhibited the least alteration in color coordinates, the highest antioxidant capacity, and the greatest retention of chlorophylls and total carotenoids. At a specific temperature, the quality of the product obtained from vacuum drying was slightly lower compared to that from freeze drying. The most substantial changes in the physicochemical properties of the dried product were observed with convection-microwave drying at a microwave power of 100 W. The drying method selected had a significant impact on the energy consumption of grinding, average particle size, and the grinding energy index of the dried celery stems; these parameters worsened as the drying temperature increased. The product with the best quality characteristics and disintegration parameters was achieved using freeze drying at 40 °C.
Study on the Changes and Correlation of Microorganisms and Flavor in Different Processing Stages of Mianning Ham
Authors: Huang Yue , Wang Zhengli , Gan Ling , Zhang Jiamin , Wang Wei , Ji Lili , Chen Lin
Published: August 2024

(1) Background: Mianning ham is a dry-cured ham from Southwest China, known for its distinct regional characteristics and delicious taste. It is particularly favored by consumers due to its safety, as no artificial nitrites are added during processing. The microbial changes during its different processing stages significantly influence the final product’s flavor. This study aims to investigate the changes in microbial communities and flavor compounds across the nine stages of Mianning ham production, from raw material preparation to salting, drying, fermentation, and maturation, using 16S and ITS sequencing, as well as solid-phase microextraction–gas chromatography–mass spectrometry (SPME-GC-MS). The goal is to explore the correlation between these changes and provide a basis for process improvement from the initial raw material preparation. (2) Results: The microbiota of Mianning ham across different processing stages mainly consisted of Proteobacteria, Firmicutes, and Ascomycota. A total of 324 volatile compounds were identified, of which 27 were key contributors to the ham’s flavor. Aldehydes contributed the most to flavor, with octanal, trans-2-nonenal, and trans, trans-2,4-decadienal being the most significant contributors at various stages. Mature Mianning ham, fermented for 1–3 years, exhibited fresh grass and earthy aromas, buttery and fatty flavors, and a distinctive roasted potato note. Correlation analysis revealed that Cobetia was the primary bacterial contributor to the main flavor compounds, especially prominent in the second year of fermentation. Among fungi, Yamadazyma and Aspergillus positively influenced several key aldehyde flavor compounds throughout the processing stages, significantly contributing to the flavor profile of Mianning ham. (3) Conclusions: Correlation analysis showed that the Mianning ham that matured for two years had the richest and most characteristic flavor. The positive and consistent impact of fungi on the ham’s flavor suggests that they may warrant further research and application in Mianning ham production. This provides a theoretical basis for improving the flavor quality and enhancing the market competitiveness of Mianning ham. One of the key features of Mianning ham is its substantial accumulation of hydrocarbons, which surpasses that of hams from other regions in China. A notable characteristic of Mianning ham processing is the absence of artificially added nitrites as antioxidants and color fixatives. Whether this absence is a contributing factor to the significant accumulation of hydrocarbons warrants further investigation.
Critical Review of Food Colloidal Delivery System for Bioactive Compounds: Physical Characterization and Application
Authors: Wang Bijie , LvYe Jiayi , Yang Shaoming , Shi Ying , Chen Qihe
Published: August 2024

Bioactive compounds (BACs) have attracted much attention due to their potential health benefits. However, such substances have problems such as difficulty dissolving in water, poor stability, and low intestinal absorption, leading to serious limitations in practical applications. Nowadays, food colloidal delivery carriers have become a highly promising solution due to their safety, controllability, and efficiency. The use of natural macromolecules to construct delivery carriers can not only regulate the solubility, stability, and intestinal absorption of BACs but also effectively enhance the nutritional added value of functional foods, improve sensory properties, and extend shelf life. Moreover, smart-responsive colloidal delivery carriers can control the release characteristics of BACs, thus improving their absorption rate in the human body. This review describes the characteristics of several typical food colloid delivery carriers, focuses on their physical properties from static structure to dynamic release, summarizes their applications in delivery systems, and provides an outlook on the future development of food colloid delivery carriers. The different compositions and structures of food colloids tend to affect their stability and release behaviors, and the different surface properties and rheological characteristics of the carriers predestine their different application scenarios. The control of in vivo release properties and the effect on food media should be emphasized in the future exploration of safer and more controllable carrier systems.
Evaluating Wind Speed Forecasting Models: A Comparative Study of CNN, DAN2, Random Forest and XGBOOST in Diverse South African Weather Conditions
Authors: Mugware Fhulufhelo Walter , Sigauke Caston , Ravele Thakhani
Published: August 2024

The main source of electricity worldwide stems from fossil fuels, contributing to air pollution, global warming, and associated adverse effects. This study explores wind energy as a potential alternative. Nevertheless, the variable nature of wind introduces uncertainty in its reliability. Thus, it is necessary to identify an appropriate machine learning model capable of reliably forecasting wind speed under various environmental conditions. This research compares the effectiveness of Dynamic Architecture for Artificial Neural Networks (DAN2), convolutional neural networks (CNN), random forest and XGBOOST in predicting wind speed across three locations in South Africa, characterised by different weather patterns. The forecasts from the four models were then combined using quantile regression averaging models, generalised additive quantile regression (GAQR) and quantile regression neural networks (QRNN). Empirical results show that CNN outperforms DAN2 in accurately forecasting wind speed under different weather conditions. This superiority is likely due to the inherent architectural attributes of CNNs, including feature extraction capabilities, spatial hierarchy learning, and resilience to spatial variability. The results from the combined forecasts were comparable with those from the QRNN, which was slightly better than those from the GAQR model. However, the combined forecasts were more accurate than the individual models. These results could be useful to decision-makers in the energy sector.
Feedback Control Design Strategy for Stabilization of Delayed Descriptor Fractional Neutral Systems with Order 0 < ϱ < 1 in the Presence of Time-Varying Parametric Uncertainty
Authors: Aghayan Zahra Sadat , Alfi Alireza , Pahnehkolaei Seyed Mehdi Abedi , Lopes António M.
Published: August 2024

Descriptor systems are more complex than normal systems, which are modeled by differential equations. This paper derives stability and stabilization criteria for uncertain fractional descriptor systems with neutral-type delay. Through the Lyapunov–Krasovskii functional approach, conditions subject to time-varying delay and parametric uncertainty are formulated as linear matrix inequalities. Based on the established criteria, static state- and output-feedback control laws are designed to ensure regularity and impulse-free properties, together with robust stability of the closed-loop system under permissible uncertainties. Numerical examples illustrate the effectiveness of the control methods and show that the results depend on the range of variation in the delays and on the fractional order, leading to stability analysis results that are less conservative than those reported in the literature.
A Preliminary Hazard Assessment of Kolumbo Volcano (Santorini, Greece)
Authors: Katsigera Anna , Nomikou Paraskevi , Pavlopoulos Kosmas
Published: August 2024

Volcanic eruptions stand as destructive threats to adjacent communities, unleashing multiple hazards such as earthquakes, tsunamis, pyroclastic flows, and toxic gases. The imperative for proactive management of volcanic risks and communities’ adaptation cannot be overstated, particularly in densely populated areas where the potential for widespread devastation looms large. Kolumbo, an active submarine volcano located approximately 7 km northeast of Santorini Island in Greece, serves as a pertinent case. Its historical record is characterised by an eruption in 1650 CE that produced a catastrophic tsunami. The aftermath witnessed havoc on neighbouring islands, coupled with casualties stemming from noxious gases in Santorini. Eyewitness accounts mention maximum water run-up heights of 20 m on the southern coast of Ios, inundation of an area of 240 m inland on Sikinos, and a flooding of up to 2 km2 inland on the eastern coast of Santorini. Recent studies suggest that a potential future eruption of Kolumbo poses a substantial hazard to the northern and eastern coasts of Santorini. Unfortunately, the absence of a concrete management protocol leaves these areas vulnerable to an impending threat that demands immediate attention. Therefore, it is recommended that a comprehensive approach be adopted, involving scientific research (active monitoring, hazard maps), community engagement, preparedness planning with government agencies, and the development of timely response strategies to reduce the associated risks, prevent casualties, and mitigate the potential consequences on the region’s economy and infrastructure.
“And the Script Sounds”: Literary Hermeneutics and Imaginary Listening
Authors: Goebel Rolf J.
Published: August 2024

Friedrich Hölderlin’s late hymns Patmos (first version) and Mnemosyne (early draft) create an intriguing tension between the “solid letter” that must be deciphered faithfully and the evocation of a “sounding script” that, together with an equally enigmatic “echo”, refuses direct hermeneutic understanding. At the point where the reader’s interpretive desire threatens to fail, musical settings like Peter Ruzicka’s MNEMOSYNE: Remembrance and Forgetting can be listened to as an attempt to actualize what Hölderlin’s original writing must leave unrealizable: the presence of real sound. In this audio-hermeneutic transfer, the act of listening opens up possibilities of the audible that are promised by the literary text without being actualized. The present essay interrogates this intermedial translatability between letter and sound by isolating a few selected passages from the facsimile reproduction of Hölderlin’s palimpsestic manuscript of multiple revisions, as provided by the Frankfurter Ausgabe. Mindful of the discontinuities and gaps in the original poems, my own analysis foregrounds its own fragmentary mode of reading Hölderlin’s poetry and listening to Ruzicka’s music.
Utility of Stool-Based Tests for Colorectal Cancer Detection: A Comprehensive Review
Authors: Gómez-Molina Raquel , Suárez Miguel , Martínez Raquel , Chilet Marifina , Bauça Josep Miquel , Mateo Jorge
Published: August 2024

Colorectal cancer (CRC) is a significant global health issue where early detection is crucial for improving treatment outcomes and survival rates. This comprehensive review assesses the utility of stool-based tests in CRC screening, including traditional fecal occult blood tests (FOBT), both chemical (gFOBT) and immunochemical techniques (FIT), as well as multitarget stool DNA (mt-sDNA) as a novel and promising biomarker. The advancements, limitations and the impact of false positives and negatives of these methods are examined. The review analyzed various studies on current screening methods, focusing on laboratory tests and biomarkers. Findings indicate that while FIT and mt-sDNA tests offer enhanced sensitivity and specificity over traditional guaiac-based FOBT, they also come with higher costs and potential for increased false positives. FIT shows better patient adherence due to its ease to use, but incorrect usage and interpretation of FOBT can lead to significant diagnostic errors. In conclusion, despite the improvements in FOBT methods like FIT in CRC detection, careful consideration of each method’s benefits and drawbacks is essential. Effective CRC screening programs should combine various methods tailored to specific population needs, aiming for early detection and reduced mortality rates.
Improving Willingness to Care and Training Needs for PLWHA from the Perspective of Student Nurses in China: A Qualitative Study
Authors: Shi Chunhong , Cleofas Jerome V.
Published: August 2024

People living with HIV and AIDS (PLWHA) deserve equitable and high-quality care. Current HIV and AIDS nursing education may not adequately prepare student nurses for the complexities of caring for PLWHA, and the perspectives of student nurses have not been sufficiently revealed in nursing education research. This study aimed to explore the viewpoints of student nurses with AIDS care experience on methods to improve their care willingness for PLWHA and to identify their educational and training needs. A descriptive qualitative study design was employed, interviewing 18 undergraduate student nurses from 14 tertiary hospitals across 7 provinces in China. Content analysis of transcripts revealed insightful suggestions for improving nursing students’ willingness, such as increased HIV and AIDS education and training, psychological preparation, positive role modeling, raising awareness about AIDS patients, and fostering nursing professionalism. Highlighted education and training needs include progress in HIV and AIDS treatment, preventive measures, psychological support for PLWHA, post-exposure protocols, and HIV-infected risk behaviors. These findings highlight the need for HIV and AIDS education, psychological support training, and stigma-reduction strategies. This study provides valuable insights that could inform policymakers, educators, and healthcare providers on preparing future nurses to meet the complex needs of PLWHA.
Does Robotic Assisted Technique Improve Patient Utility in Total Knee Arthroplasty? A Comparative Retrospective Cohort Study
Authors: Ratti Matteo , Ceriotti Daniele , Rescinito Riccardo , Bibi Rabia , Panella Massimiliano
Published: August 2024

Background: Even if robotic assisted total knee arthroplasty (RATKA) is a widely used technique, there is still a gap of knowledge about whether this technology is effective in improving the patient utility. This measure is of paramount importance for conducting cost effectiveness analysis. The aim of this study was to compare the utility measure derived from self-reported outcomes questionnaires in patients who underwent RATKA compared to patients who underwent the manual surgery. Methods: We compared 72 patients operated with a robotic technique with 70 operated with traditional technique. The utility data were collected with the WOMAC (Western Ontario and McMaster University Osteoarthritis index) self-administrated questionnaire that investigates pain, stiffness and functionality of the patients, an then mapped to a utility value through a validated transforming function. We performed three surveys: the first one before the intervention (t0), the second one 1 year after the surgery (t1) and the third one at the 2 year follow up (t2). Results: we observed higher utility values in both groups. In detail, the mean utility score in the RATKA group increased from 0.37 to 0.71 (t1) and 0.78 (t2), while in the conventional group it increased from 0.41 to 0.78 (t1) and 0.78 (t2). The fixed effect coefficients of t1 and t2 were found to be 0.37 and 0.363 (p < 0.001 for both). The coefficient of the robotic technique, along with its interaction with the t1 and t2 time effect was non-significant. Conclusions: Even if at t1 the utility of patient who underwent RATKA were lower, at longer follow up (t2) we found no significant difference compared to traditional technique, leaving the superiority of robotic assisted technique yet to be proved. Our results may be useful for calculating the gained or lost Quality Adjusted Life Years (QALYs), so that the health care system (or an insurance company) could make an appropriate decision whether to fund the robotic approach or not, after a careful assessment of the incremental costs incurred.
Quantifying Uncertainty in Hypothetical 3D Reconstruction—A User-Independent Methodology for the Calculation of Average Uncertainty
Authors: Foschi Riccardo , Fallavollita Federico , Apollonio Fabrizio Ivan
Published: August 2024

A shared commitment to standardising the process of hypothetically reconstructing lost buildings from the past has characterised academic research in recent years and can manifest at various stages of the reconstructive process and with different perspectives. This research specifically aims to establish a user-independent and traceable procedure that can be applied at the end of the reconstructive process to quantify the average level of uncertainty of 3D digital architectural models. The procedure consists of applying a set of mathematical formulas based on numerical values retrievable from a given scale of uncertainty and developed to simplify reuse and improve transparency in reconstructive 3D models. This effort to assess uncertainty in the most user-independent way possible will contribute to producing 3D models that are more comparable to each other and more transparent for academic researchers, professionals, and laypersons who wish to reuse them. Being able to calculate a univocal numerical value that gives information on the global average uncertainty of a certain reconstructive model is an additional synthetic way, together with the more visual false-colour scale of uncertainty, to help disseminate the work in a clear and transmissible way. However, since the hypothetical reconstructive process is a process based on personal interpretation, which inevitably requires a certain level of subjectivity, it is crucial to define a methodology to assess and communicate this subjectivity in a user-independent and reproducible way.
Risk of Economic Violence: A New Quantification
Authors: D’Agostino Federica , Zacchia Giulia , Corsi Marcella
Published: August 2024

This paper defines the first internationally comparable measure of the risk of economic violence to acknowledge its prevalence in different countries and its geographical and gender heterogeneity. Thanks to the availability of micro-data from the OECD/International Network on Financial Education survey, currently used to track financial literacy in different countries, we define a measure of the risk of economic violence (REV) that takes into consideration three macro-areas: (a) the risk of being prevented from acquiring and accumulating financial resources; (b) the risk of being unaware and not having access to personal and/or household financial resources; and (c) the risk of financial dependency. The definition of the new economic violence risk measure (REV) then allows us to verify with real data the presence of women’s greater exposure to the risk of economic violence and the presence of gender differences in the determinants of economic violence risk. Finally, we verify that financial literacy protects individuals from the risk of economic violence, without gender differences.
SAMPLID: A New Supervised Approach for Meaningful Place Identification Using Call Detail Records as an Alternative to Classical Unsupervised Clustering Techniques
Authors: Mendoza-Hurtado Manuel , Romero-del-Castillo Juan A. , Ortiz-Boyer Domingo
Published: August 2024

Data supplied by mobile phones have become the basis for identifying meaningful places frequently visited by individuals. In this study, we introduce SAMPLID, a new Supervised Approach for Meaningful Place Identification, based on providing a knowledge base focused on the specific problem we aim to solve (e.g., home/work identification). This approach allows to tackle place identification from a supervised perspective, offering an alternative to unsupervised clustering techniques. These clustering techniques rely on data characteristics that may not always be directly related to classification objectives. Our results, using mobility data provided by call detail records (CDRs) from Milan, demonstrate superior performance compared to applying clustering techniques. For all types of CDRs, the best results are obtained with the 20 × 20 subgrid, indicating that the model performs better when supplied with information from neighboring cells with a close spatial relationship, establishing neighborhood relationships that allow the model to clearly learn to identify transitions between cells of different types. Considering that it is common for a place or cell to be labeled in multiple categories at once, this supervised approach opens the door to addressing the identification of meaningful places from a multi-label perspective, which is difficult to achieve using classical unsupervised methods.
Protein Charge Neutralization Is the Proximate Driver Dynamically Tuning Reflectin Assembly
Authors: Levenson Robert , Malady Brandon , Lee Tyler , Al Sabeh Yahya , Gordon Michael J. , Morse Daniel E.
Published: August 2024

Reflectin is a cationic, block copolymeric protein that mediates the dynamic fine-tuning of color and brightness of light reflected from nanostructured Bragg reflectors in iridocyte skin cells of squids. In vivo, the neuronally activated phosphorylation of reflectin triggers its assembly, driving osmotic dehydration of the membrane-bounded Bragg lamellae containing the protein to simultaneously shrink the lamellar thickness and spacing while increasing their refractive index contrast, thus tuning the wavelength and increasing the brightness of reflectance. In vitro, we show that the reduction in repulsive net charge of the purified, recombinant reflectin—either (for the first time) by generalized anionic screening with salt or by pH titration—drives a finely tuned, precisely calibrated increase in the size of the resulting multimeric assemblies. The calculated effects of phosphorylation in vivo are consistent with these effects observed in vitro. The precise proportionality between the assembly size and charge neutralization is enabled by the demonstrated rapid dynamic arrest of multimer growth by a continual, equilibrium tuning of the balance between the protein’s Coulombic repulsion and short-range interactive forces. The resulting stability of reflectin assemblies with time ensures a reciprocally precise control of the particle number concentration, encoding a precise calibration between the extent of neuronal signaling, osmotic pressure, and the resulting optical changes. The charge regulation of reflectin assembly precisely fine-tunes a colligative property-based nanostructured biological machine. A physical mechanism is proposed.
Gene Duplication and Functional Diversification of MADS-Box Genes in Malus × domestica following WGD: Implications for Fruit Type and Floral Organ Evolution
Authors: Wang Baoan , Xiao Yao , Yan Mengbo , Fan Wenqi , Zhu Yuandi , Li Wei , Li Tianzhong
Published: August 2024

The evolution of the MADS-box gene family is essential for the rapid differentiation of floral organs and fruit types in angiosperms. Two key processes drive the evolution of gene families: gene duplication and functional differentiation. Duplicated copies provide the material for variation, while advantageous mutations can confer new functions on gene copies. In this study, we selected the Rosaceae family, which includes a variety of fruit types and flower organs, as well as species that existed before and after whole-genome duplication (WGD). The results indicate that different fruit types are associated with different copies of MADS-box gene family duplications and WGD events. While most gene copies derived from WGD have been lost, MADS-box genes not only retain copies derived from WGD but also undergo further gene duplication. The sequences, protein structures, and expression patterns of these gene copies have undergone significant differentiation. This work provides a clear example of MADS-box genes in the context of gene duplication and functional differentiation, offering new insights into the evolution of fruit types and floral organs.
In Vitro Assay Development to Study Pulse Field Ablation Outcome Using Solanum Tuberosum
Authors: Narkar Akshay , Kaboudian Abouzar , Ardeshirpour Yasaman , Casciola Maura , Feaster Tromondae , Blinova Ksenia
Published: August 2024

Exposing cells to intense and brief electric field pulses can modulate cell permeability, a phenomenon termed electroporation. When applied in medical treatments of diseases like cancer and cardiac arrhythmias, depending on level of cellular destruction, it is also referred to as irreversible electroporation (IRE) or Pulsed Field Ablation (PFA). For ablation device testing, several pulse parameters need to be characterized in a comprehensive manner to assess lesion boundary and efficacy. Overly aggressive voltages and application numbers increase animal burden. The potato tuber is a widely used initial model for the early testing of electroporation. The aim of this study is to characterize and refine bench testing for the ablation outcomes of PFA in this simplistic vegetal model. For in vitro assays, several pulse parameters like voltage, duration, and frequency were modulated to study effects not only on 2D ablation area but also 3D depth and volume. As PFA is a relatively new technology with minimal thermal effects, we also measured temperature changes before, during, and after ablation. Data from experiments were supplemented with in silico modeling to examine E-field distribution. We have estimated the irreversible electroporation threshold in Solanum Tuberosum to be at 240 V/cm. This bench testing platform can screen several pulse recipes at early stages of PFA device development in a rapid and high-throughput manner before proceeding to laborious trials for IRE medical devices.
Factors Determining Epithelial-Mesenchymal Transition in Cancer Progression
Authors: Tomecka Paulina , Kunachowicz Dominika , Górczyńska Julia , Gebuza Michał , Kuźnicki Jacek , Skinderowicz Katarzyna , Choromańska Anna
Published: August 2024

Epithelial-mesenchymal transition (EMT) is a process in which an epithelial cell undergoes multiple modifications, acquiring both morphological and functional characteristics of a mesenchymal cell. This dynamic process is initiated by various inducing signals that activate numerous signaling pathways, leading to the stimulation of transcription factors. EMT plays a significant role in cancer progression, such as metastasis and tumor heterogeneity, as well as in drug resistance. In this article, we studied molecular mechanisms, epigenetic regulation, and cellular plasticity of EMT, as well as microenvironmental factors influencing this process. We included both in vivo and in vitro models in EMT investigation and clinical implications of EMT, such as the use of EMT in curing oncological patients and targeting its use in therapies. Additionally, this review concludes with future directions and challenges in the wide field of EMT.
Melanin as a Photothermal Agent in Antimicrobial Systems
Authors: Menichetti Arianna , Mordini Dario , Montalti Marco
Published: August 2024

Bacterial infection is one of the most problematic issues for human health and the resistance of bacteria to traditional antibiotics is a matter of huge concern. Therefore, research is focusing on the development of new strategies to efficiently kill these microorganisms. Recently, melanin is starting to be investigated for this purpose. Indeed, this very versatile material presents outstanding photothermal properties, already studied for photothermal therapy, which can be very useful for the light-induced eradication of bacteria. In this review, we present antibacterial melanin applications based on the photothermal effect, focusing both on the single action of melanin and on its combination with other antibacterial systems. Melanin, also thanks to its biocompatibility and ease of functionalization, has been demonstrated to be easily applicable as an antimicrobial agent, especially for the treatment of local infections.
Lupus Nephritis from Pathogenesis to New Therapies: An Update
Authors: Roveta Annalisa , Parodi Emanuele Luigi , Brezzi Brigida , Tunesi Francesca , Zanetti Valentina , Merlotti Guido , Francese Alessia , Maconi Antonio G. , Quaglia Marco
Published: August 2024

Lupus Nephritis (LN) still represents one of the most severe complications of Systemic Lupus Erythematosus (SLE) and a major risk factor for morbidity and mortality. However, over the last few years, several studies have paved the way for a deeper understanding of its pathogenetic mechanisms and more targeted treatments. This review aims to provide a comprehensive update on progress on several key aspects in this setting: pathogenetic mechanisms of LN, including new insight into the role of autoantibodies, complement, vitamin D deficiency, and interaction between infiltrating immune cells and kidney resident ones; the evolving role of renal biopsy and biomarkers, which may integrate information from renal histology; newly approved drugs such as voclosporin (VOC) and belimumab (BEL), allowing a more articulate strategy for induction therapy, and other promising phase III-immunosuppressive (IS) agents in the pipeline. Several adjunctive treatments aimed at reducing cardiovascular risk and progression of chronic renal damage, such as antiproteinuric agents, represent an important complement to IS therapy. Furthermore, non-pharmacological measures concerning general lifestyle and diet should also be adopted when managing LN. Integrating these therapeutic areas requires an effort towards a holistic and multidisciplinary approach. At the same time, the availability of an increasingly wider armamentarium may translate into improvements in patient’s renal outcomes over the next decades.
Carotenoid Supplementation for Alleviating the Symptoms of Alzheimer’s Disease
Authors: Flieger Jolanta , Forma Alicja , Flieger Wojciech , Flieger Michał , Gawlik Piotr J. , Dzierżyński Eliasz , Maciejewski Ryszard , Teresiński Grzegorz , Baj Jacek
Published: August 2024

Alzheimer’s disease (AD) is characterized by, among other things, dementia and a decline in cognitive performance. In AD, dementia has neurodegenerative features and starts with mild cognitive impairment (MCI). Research indicates that apoptosis and neuronal loss occur in AD, in which oxidative stress plays an important role. Therefore, reducing oxidative stress with antioxidants is a natural strategy to prevent and slow down the progression of AD. Carotenoids are natural pigments commonly found in fruits and vegetables. They include lipophilic carotenes, such as lycopene, α- and β-carotenes, and more polar xanthophylls, for example, lutein, zeaxanthin, canthaxanthin, and β-cryptoxanthin. Carotenoids can cross the blood–brain barrier (BBB) and scavenge free radicals, especially singlet oxygen, which helps prevent the peroxidation of lipids abundant in the brain. As a result, carotenoids have neuroprotective potential. Numerous in vivo and in vitro studies, as well as randomized controlled trials, have mostly confirmed that carotenoids can help prevent neurodegeneration and alleviate cognitive impairment in AD. While carotenoids have not been officially approved as an AD therapy, they are indicated in the diet recommended for AD, including the consumption of products rich in carotenoids. This review summarizes the latest research findings supporting the potential use of carotenoids in preventing and alleviating AD symptoms. A literature review suggests that a diet rich in carotenoids should be promoted to avoid cognitive decline in AD. One of the goals of the food industry should be to encourage the enrichment of food products with functional substances, such as carotenoids, which may reduce the risk of neurodegenerative diseases.
Toxic Features and Metabolomic Intervention of Glabrene, an Impurity Found in the Pharmaceutical Product of Glabridin
Authors: Li Xue , Jiang Haixin , Guo Dongxue , Huang Wen , Ren Houpu , Zhang Qiang
Published: August 2024

Glabridin is a widely used product in the cosmetics and pharmaceutical industry, which is generally isolated and purified from Licorice (Glycyrrhiza glabra) extract in industrial production. It has wide clinical applications, but significant toxicity has also been reported. The purity of glabridin raw material is generally between 90% and 98%. We have identified a toxic impurity, glabrene, in the industrial product glabridin. Our investigation using an AB wild-type zebrafish toxicity test showed that glabrene has a significant lethal effect with an LC10 of 2.8 μM. Glabrene induced obvious malformation and disrupted cartilage development in zebrafish larvae. Furthermore, the compound significantly reduced larval mobility and caused damage to brain neural tissues. Metabolic pathway analysis and neurotransmitter quantification via ELISA indicated abnormal activation of the phenylalanine metabolic pathway, resulting in elevated dopamine and acetylcholine levels in vivo. These findings provide insights into the potential risks of glabrene contamination and offer a new reference point for enhancing safety measures and quality controls in licorice-derived products.
Burn Wound Healing Activity of Hydroxyethylcellulose Gels with Different Water Extracts Obtained from Various Medicinal Plants in Pseudomonas aeruginosa-Infected Rabbits
Authors: Demyashkin Grigory , Sataieva Tatiana , Shevkoplyas Ludmila , Kuevda Tatyana , Ahrameeva Maria , Parshenkov Mikhail , Mimuni Alexander , Pimkin Georgy , Atiakshin Dmitrii , Shchekin Vladimir , Shegay Petr , Kaprin Andrei
Published: August 2024

Burn injuries represent a significant problem in clinical practice due to the high risk of infection and the prolonged healing process. Recently, more attention has been given to natural remedies such as water extracts of various medicinal plants, which possess anti-inflammatory and wound healing properties. The aim of this study is to evaluate the efficacy and safety of Satureja montana L. and other water extracts in a burn wound model. The study involved male Californian rabbits (n = 52) divided into eight groups. Burn wounds were modeled on the animals and subsequently treated with gels based on Satureja montana L. and other water extracts. The reparative potential of the epidermis (assessed by Ki-67 expression), the state of local immunity (measured by the number of CD-45 cells), and the anti-inflammatory role of mast cells (measured by tryptase levels) were evaluated. Bacteriological and morphological studies were conducted. The most pronounced bactericidal, reparative, and immunostimulatory effects were observed after the treatment using a gel mixture of water extracts from Satureja montana L., Salvia sclarea, Coriandrum sativum L., and Lavandula angustifolia in equal proportions (1:1:1:1). The other gels also demonstrated high efficacy in treating burn wounds, especially when using a strain of Pseudomonas aeruginosa resistant to several antibiotics. Immunohistochemical studies showed a significant increase in the number of Ki-67-positive cells in the basal layer of the epidermis and a decrease in the number of CD-45-positive cells, indicating improved proliferative activity and reduced inflammation. This study confirms the hypothesis that the use of water extract mixtures significantly enhances the reparative potential, improves the immune response in the treatment of burns, and promotes wound healing. These findings pave the way for further research and the application of complex phytotherapeutic agents, specifically water extracts of medicinal plants containing phenols and antioxidants in burn wound therapy.
Chronic Neuronal Hyperexcitation Exacerbates Tau Propagation in a Mouse Model of Tauopathy
Authors: Nishida Itaru , Yamada Kaoru , Sakamoto Asami , Wakabayashi Tomoko , Iwatsubo Takeshi
Published: August 2024

The intracerebral spread of tau is a critical mechanism associated with functional decline in Alzheimer’s disease (AD) and other tauopathies. Recently, a hypothesis has emerged suggesting that tau propagation is linked to functional neuronal connections, specifically driven by neuronal hyperactivity. However, experimental validation of this hypothesis remains limited. In this study, we investigated how tau propagation from the entorhinal cortex to the hippocampus, the neuronal circuit most susceptible to tau pathology in AD, is affected by the selective stimulation of neuronal activity along this circuit. Using a mouse model of seed-induced propagation combined with optogenetics, we found that the chronic stimulation of this neuronal connection over a 4-week period resulted in a significant increase in insoluble tau accumulation in both the entorhinal cortex and hippocampus. Importantly, the ratio of tau accumulation in the hippocampus relative to that in the entorhinal cortex, serving as an indicator of transcellular spreading, was significantly higher in mice subjected to chronic stimulation. These results support the notion that abnormal neuronal activity promotes tau propagation, thereby implicating it in the progression of tauopathy.