bluuebunny/crossref_metadata_2025 huggingface dataset:
Manejo de los síntomas dolorosos de la endometriosis con dispositivo intrauterino de levonorgestrel en una paciente con pelvis congelada: reporte de caso
Authors: De los Ríos José Fernando , Molina Erika
Published: April 2020

El manejo de los síntomas dolorosos de la endometriosis es un tema controvertido en nuestra especialidad. La mayoría de los tratamientos utilizados ofrecen diferentes grados de mejoría a las pacientes mientras los reciben, tienen múltiples efectos secundarios y es previsible una recaída pronto en el tiempo siguiente a su descontinuación. Muchos de los medicamentos utilizados son costosos y dados sus efectos adversos no deben ser utilizados por mucho tiempo. En algunas pacientes con paridad satisfecha la anexo-histerectomía es una alternativa terapéutica para mejorar la calidad de vida cuando han sufrido la enfermedad durante toda su etapa reproductiva. En casos avanzados de endometriosis, dicho tipo de cirugía puede ser difícil y poner en riesgo de daño a los órganos pélvicos. Describimos el caso de una paciente en quien fue imposible practicar la cirugía optándose por manejar sus síntomas con un dispositivo intrauterino de levonorgestrel (Mirena®) con un resultado satisfactorio.
Medición del consumo de oxígeno del miocardio en el puerperio normal: ejemplo de eficiencia fisiológica
Authors: Castro Alejandro , Barriga Adriana Paola , Perdomo Omar
Published: April 2020

Objetivo: determinar el consumo de oxígeno del miocardio (MVO2) en pacientes durante el puerperio temprano y comparar los resultados con los valores de MVO2 en adultos sanos publicados en otros estudios.Materiales y métodos: el MVO2 fue medido en 120 mujeres durante el puerperio temprano y sin ninguna patología agregada. La recolección de los datos se hizo en dos fases, durante las que se obtuvo un grupo retrospectivo y luego un grupo prospectivo. Se hizo un análisis de subgrupos según edad, número de partos y vía del parto. La formula EDOR fue empleada para los cálculos. Los resultados fueron comparados con los reportados para adultos sanos. Resultados: el MVO2 promedio de nuestro estudio fue 6,58 ml/min-1/100 g-1 de tejido, con un valor máximo de 7,33 ml/min-1/100 g-1 de tejido; estos resultados fueron significativa y estadísticamente menores en comparación con los reportados en adultos sanos. No hubo diferencias significativas en el MVO cuando se hizo el análisis en subgrupos según edad, número de partos o vía del parto. Conclusión: durante el puerperio, se observa gran eficiencia cardiaca, dada por aumento del gasto cardiaco con disminución del consumo de oxígeno del miocardio.
Predictors of increased blood pressure in women after induction of superovulation for extracorporeal fertilization
Authors: Vakareva V. V. , Avdeeva M. V. , Scheglova L. V.
Published: April 2020

Objective is to study the relationship of hemodynamic, hormonal and metabolic changes with increased blood pressure (BP) in women after the induction of superovulation for extracorporeal fertilization (ECF).

Design and methods. We examined 80 women aged from 25 to 38 years (mean age 32,3 ± 3,6 years). All patients underwent ECF at the Center for Reproductive Technologies of the St Petersburg Mariinsky Hospital. Daily monitoring of BP was carried out twice: before the induction of superovulation for ECF and on the first day after this manipulation.

Results. After induction of superovulation, an increase in average daily BP ≥ 135/85 mm Hg occurred in 52,5 % of women and an increase in average night BP ≥ 120/70 mm Hg was registered in 13,8 %. In women with initially increased average daily BP, the relative risk of its further increase on the first day after induction of superovulation was 1,46 (95 % confidence interval (CI) 0,79–2,68) compared with women who had normal average daily BP. In women with initially elevated average nightly BP, the relative risk of its further increase after induction of superovulation was 1,16 (95 % CI 1,06–1,27). An increase of BP after induction of superovulation may be associated with age, female factor infertility, the presence of chronic diseases of the genitourinary system in past, changes in the metabolic profile (increase in total cholesterol, urea, creatinine, glucose, estradiol; decreased thyroid-stimulating hormone level), heart rhythm disturbances, episodes of apnea/hypopnea during sleep, increase in heart rate, increased sympathetic activity.

Conclusions. Induction of superovulation during in vitro fertilization has an adverse effect on the circadian BP profile. While planning in vitro fertilization, the risk factors for BP increase should be identified and modified. This will help to avoid adverse cardiovascular reactions associated with the procedure. Women with the hypertensive reaction after the induction of superovulation require dynamic BP monitoring.
Highly effective lipid-lowering therapy on local vascular stiffness and symptoms of chronic heart failure in patients with postinfarction cardiosclerosis
Authors: Oleynikov V. E. , Khromova A. A. , Abramova E. A. , Salyamova L. I. , Babina A. V. , Tomashevsky N. A.
Published: April 2020

Objective. To study the effect of achieving the target level of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL–C) on the parameters of local vascular stiffness and the development of chronic heart failure (CHF) in patients with ST-elevation myocardial infarction (STEMI).

Design and methods. The study included 80 patients with myocardial infarction with ST segment elevation (STEMI) aged 35 to 65 years. On the 7–9th day of STEMI, at the 24th and 48th weeks of treatment with atorvastatin 40–80 mg clinical symptoms of heart failure were analyzed, the lipid profile and brain natriuretic peptide (BNP) were determined. Ultrasound of the common carotid arteries using high-frequency RF signal technology was performed. Depending on the effectiveness of treatment with atorvastatin, patients were retrospectively divided into 2 groups: 40 people who reached the target level of LDL–C (highly effective therapy, HET) and 37 patients who did not reach the target level of LDL–C (relatively effective therapy, RET).

Results. In patients who reached the target LDL–С level, the initial BNP values were 115,2 pg/ml, with the regression by 34,5 % at follow-up (p = 0,03). There was no significant change in the RET group. In the HET group, a decrease in IMT, an improvement in distensibility parameters, a decrease in local PWV and stiffness index of common carotid arteries, were found. Based on the 6-minute walk test, only patients in HET group showed an increase in exercise tolerance after 24th week (p = 0,04). Also, individuals who did not reach the target LDL–С level, showed a decrease in the quality of life 27,4 % (p = 0,03). Patients in the HET group showed no negative dynamics.

Conclusion. We showed that at the long-term follow-up after STEMI, the achievement of the target LDL cholesterol values was accompanied by a significant improvement in the parameters of local rigidity of the main arteries, and a decrease in BNP level, as well as by more favorable course of CHF.
Sleep-related breathing disorders in patients with pulmonary arterial hypertension and chronic thromboembolic pulmonary hypertension
Authors: Dyachenko M. A. , Simakova M. A. , Korostovtseva L. S. , Bochkarev M. V. , Goncharova N. S. , Sviryaev Yu. V. , Moiseeva O. M.
Published: April 2020

Background.The data evidence that in patients with pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) nocturnal hypoxemia is associated with poor prognosis. Although, data regarding sleep-related breathing disorders (SRBD) prevalence and their association with pulmonary hypertension (PH) severity are scarce.

Objective.To evaluate the prevalence and the structure of SRBD in patients with PAH and CTEPH and the relationship of SRBD with PH severity. Design and methods. In a prospective, single-center study we examined 31 patients (45 % male (n = 14)) with a verified diagnosis of precapillary PH: 22,6 % with IPAH; 9,7 % with PAH associated with congenital heart disease; 64,5 % with CTEPH; 3,2 % with PAH associated with connective tissue disease. Patients underwent a general clinical examination, questionnaires, respiratory tests, full videopolysomnography, electrocardiogram, and heart ultrasound (ECHO) examination, clinical and biochemical blood tests, including the assessment of ADMA and NT-proBNP levels.

Results.No differences in SRBD pattern in patients with PAH and CTEPH were observed as well as with the severity of PH. A positive correlation was found between the apnea-hypopnea index (AHI) and the end-diastolic left ventricular dimension (ρ = 0,54; p = 0,005); the ventricular diameter ratio (RV/LV) negatively correlated with AHI (ρ = –0,41; p = 0,05). Low peripheral blood oxygen saturation negatively correlated with NT-proBNP level (ρ = –0,40; p = 0.035). ADMA level was increased in all patients, nevertheless no association between ADMA and SRBD severity was found (χ2= 2,97; p = 0,085).

Conclusions.SRBD often occurs among patients with PAH and CTEPH, while the presence of SRBD is not associated with the severity of PH. The severity of SRBD is associated with left heart remodeling. The severity of nocturnal hypoxemia in our group is associated with the increased NT-proBNP level, which is consistent with the idea of a negative prognostic value of nocturnal hypoxemia in patients with PAH and CTEPH.
Biofungicides evaluation in two coffee cultivars for Hemileia vastatrix control
Authors: Fajardo-Franco Marja Liza , Aguilar-Tlatelpa Martin , Guzmán-Plazola Remigio A.
Published: April 2020

El objetivo del presente estudio fue evaluar el efecto de biofungicidas elaborados con Azadirachta indica, Melaleuca alternifolia y la combinación de Bacillus subtilis con A. indica y Syzygium aromaticum, en el control de la roya causada por Hemileia vastatrix, en las variedades Garnica y Typica. Se establecieron experimentos en condiciones de campo en tres localidades: Lipuntahuaca, Chilocoyo y Cinco de Mayo, con plantas de cinco meses de edad. Se encontraron diferencias significativas entre biofungicidas y el testigo para la incidencia (P=0.04), área bajo la curva del progreso de la severidad (ABCPE) (P=0.04) y tasa de infección aparente (P=0.03). No se encontraron diferencias en severidad excepto en la última evaluación. M. alternifolia indujo los valores más bajos de incidencia (34.4 %), ABCPE (61.8) y tasa de infección aparente (0.028), con porcentajes de reducción con respecto al testigo de 20.9, 14.2 y 39.1%, respectivamente. No se presentaron diferencias significativas entre las variedades Typica y Garnica para incidencia y severidad. Las plantas de la localidad de Lipuntahuaca tuvieron menor incidencia (26.9%) y severidad (2.3%) en comparación con Chilocoyo y Cinco de Mayo con 51.5 y 44.5 % (incidencia) y 4.2 y 3.4% (severidad), respectivamente (P<0.0001). Los biofungicidas no suprimieron la infección, pero los resultados sugieren que debe continuarse la investigación en este patosistema.
Multiple Aplasia Cutis Congenita Lesions of the Scalp: A Case Study
Authors: Whalen Mary
Published: April 2020

Aplasia cutis congenita (ACC) is a rare condition that presents at birth as an absence of skin that does not usually involve underlying structures. Occurring in 3/10,000 live births, ACC is evenly distributed between males and females; the risk of ACC increases to 7 percent in consanguineous marriages. Up to 86 percent of lesions are found on the scalp in the midline vertex position. Lesions can also be found on the trunk and limbs, as with Adams-Oliver syndrome or accompanying epidermolysis bullosa. ACC is associated with chromosomal abnormalities and 35–50 percent of the time with trisomy 13 (Patau syndrome). This case study presents an infant with multiple ACC lesions of the scalp. The pathophysiology, treatment, potential long-term complications, and nursing considerations are discussed.
Intrathecal Opioid Dosing During Spinal Anesthesia for Cesarean Section: An Integrative Review
Authors: Gomez Nicole A. Gonzaga , Warren Nicole , Labko Yuliya , Sinclair David R.
Published: April 2020

Approximately one in three women in the United States deliver via Cesarean section (CS), making it one of the most common surgical procedures in the country. Neuraxial (spinal or epidural) anesthesia is the most effective and common anesthetic approach for pain relief during a CS in the United States and often associated with adverse effects such as nausea, vomiting, and pruritus. While recommended dose ranges exist to protect patient safety, there are a lack of guidelines for opioid doses that both optimize postoperative pain management and minimize side effects. This integrative review synthesizes the evidence regarding best practice of opioid dosing in neuraxial anesthesia for planned CS. Evidence supports the use of lower doses of intrathecal (IT) opioids, specifically 0.1 morphine, to achieve optimal pain management with minimal nausea, vomiting, and pruritus. Lower IT doses have potential to achieve pain management and to alleviate preventable side effects in women delivering via CS.
Editorial
Authors: Coutinho Luciana Cristina Salvatti
Published: April 2020

A Revista HISTEDBR On-line, fruto de uma construção coletiva e plural, conta, nesse volume 19, primeiro em que adotamos a publicação contínua, com 04 artigos internacionais, 54 artigos nacionais e 1 resenha resultante de estudos e pesquisas com temáticas e abordagens variadas realizadas por pesquisadores vinculados a diferentes instituições do Brasil e do exterior, tais como: Universidad de Holguin (Cuba), Universidad Nacional de Córdoba (Argentina), El Colegio de San Luis (México), Facultad de Ciencias de la Educación (Argentina), UFSCar, USP, UFES, Unesp, UESC, Centro Universitário Leonardo da Vinci, Unicamp, UFPA, Secretaria Municipal de Educação de Belém/PA, UFV, UEL, IFMG, Fundação Herminio Ometto, Unimep, IFNMG, Unicentro, UNISO, UERJ, Secretaria Municipal de Educação de Duque de Caxias, Secretaria Municipal de Educação de Guarapuava/PR, UNISC, UFRJ, UEM, IFRS, UFRGS, IFG, UFC, UFPel, Secretaria Municipal de Educação de Pelotas, IFCE, UECE, Secretaria da Educação do Estado do Amapá, Centro Universitário Una, UCS, UFGD, Secretaria Estadual de Educação do Paraná/PR, ETERJ, PUC-RJ, IFAL, Unioeste, IFBA, Secretaria Municipal de Educação de Farroupilha-RS, Colégio Pedro II, UnB, UFSM, UFRRJ, UESB, Secretaria Municipal de Educação de Florianópolis/SC, UDESC, IFMA, UFG, UFMA.
Revisiting Early-stage COVID-19 Strategy Options
Authors: Machanick Philip
Published: April 2020

Background: Early-stage interventions in a potential pandemic are important tounderstand as they can make the difference between runaway exponential growththat is hard to turn back and stopping the spread before it gets that far. COVID-19 is an interesting case study because there have been very different outcomesin different localities. These variations are best studied after the fact if precisionis the goal; while a pandemic is still unfolding less precise analysis is of value inattempting to guide localities in the early stages to learn lessons of those that pre-ceded them. Methods: I examine three factors that could differentiate strategy: asymptomaticspread, differences in use of the Bacillus Calmette-Guerin (BCG) tuberculosis vac-cine and cloth face masks.Results:Differences in disease progression as well as the possibility of alternativestrategies to prevent COVID-19 from entering the runaway phase or damping itdown later can be elucidated by a study of asymptomatic infection. A study todemonstrate not only what fraction are asymptomatic but how contagious they arewill also inform policy on universal mask wearing. Conclusions: While a COVID-19 outbreak is at a level that makes accurate trace-and test possible, investigation of asymptomatic transmission is viable and shouldbe attempted to enhance understanding of spread and variability in the disease aswell as policy options for slowing the spread.
Neuroinvasive Potential of A Primary Respiratory Pathogen SARS-CoV2: Exploring the Underrecognized
Authors: Lahiri Durjoy , Mondal Ritwick , Deb Shramana , Bandopadhyay Deebya , Shome Gourav , Sarkar Sukanya , Biswas Subhas C.
Published: April 2020

After the emergence of Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV) and Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the last two decades, the world is facing its new challenge in SARS-CoV-2 pandemic with unfathomable global responses. The characteristic clinical symptoms for Coronavirus (COVID-19) affected patients are high fever, dry-cough, dyspnoea, lethal pneumonia whereas some patients also show few additional neurological signs such as headache, nausea, vomiting. The accumulative evidences suggest that Coronavirus is not only confined within the respiratory tract and that may also invade in central nervous system (CNS), peripheral nervous system (PNS) inducing some fatal Neurological diseases. Here we analyse the phylogenetic perspective of SARS-CoV2 with other strains of β-Coronaviridae from a standpoint of neurological spectrum disorders. Based on the existing case reports, literature and open data-bases, we also analyse the differential distribution of neurological impairments in COVID-19 positive patients along with angiotensin-converting enzyme-2(ACE2) expression dynamics in neuronal and non-neuronal tissue of central and peripheral nervous system. Besides, we discuss the need for modulations in clinical approach from a neurological point of view, as a measure towards reducing disease transmission, morbidity and mortality in SARS-CoV2 positive patients.
REPRESENTASI DALAM PEMBELAJARAN MATEMATIKA SISWA KELAS VII MATERI HIMPUNAN SMPK WIGNYA MANDALA TUMPANG
Authors: Ngedo David Ra , Prayitno Anton - , Octavianti Cynthia Tri
Published: April 2020

ABSTRAK.

Kemampuan representasi merupakan salah satu kemampuan yang mempunyai keterkaitan dengan pemahaman matematika, representasi merupakan hal terpenting dalam mengkonstruksi ide dan pemahaman siswa terkait dengan konsep-konsep matematika. Subjek dalam penelitian ini adalah seluruh siswa kelas VII SMPK Wignya Mandala Tumpang sebanyak 18 siswa akan tetapi hanya 3 subjek yang dinalisis. Pengambilan subjek dalam penelitian ini menggunakan teknik kualitatif berdasarkan representasi kesalahan siswa. Subjek dipilih terlebih dahuluh berdasarkan pertimbangan-pertimbangan peneliti sesuai tujuan penelitian,itu subjek diambil secara acak. Berdasarkan hasil penelitian yang diperoleh menunjukkan jenis-jenis representasi dalam pembelajaran matematika, kesulitan yang berkaitan dengan kesulitan tiga objek menentukan, membaca, dan membedakan. Presentase representasi kesulitan siswa menentukan sebesar 62,0% di kategorikan sedang, kesulitan membaca sebesar 29,% di kategorikan rendah dan kesulitan membedakan sebesar 72,2%) kategori tinggi. faktor penyebabnya kesalahan representasi kemampuan siswa yaitu sulit dalam membedakan bilangan asli, kurang dari serta sulit dalam menentukan irisan, dan dalam menggambar diagram venn, dari kesalahan tersebut siswa tidak dapat menentukan himpunan penyelesaian atau siswa tersebut kesulitan dalam memahami dan mencermati.
Persepsi Orangtua tentang Bermain Berisiko pada Anak Usia Dini
Authors: Buahana Baiq Nada
Published: April 2020

Risk play is outdoors play which involves a lot of physical activity. Parents' concerns will limit the range of movement to explore when playing risky. This research explores parents 'perceptions about playing risk as viewed from the child's age, gender, and reviews parents' knowledge about playing risk. The study was conducted in Purbalingga, Indonesia. The research subjects involved 33 mothers and 33 fathers of preschool children with ages ranging from 4 to 6 years. The study findings show that mothers and fathers have the same risk playing perception. Parents provide the highest support for children's play activities at risk such as climbing and jumping from a height. As for other activities, parents have concerns about the safety of children during these activities. Judging from the age of the father and mother allow children aged 4 years with 40%, 5 years 45% and 6 years 50% while seen from jrniskelmin, parents allow boys more than girls to play at risk with percentage of 55% compared to 40%. Perceptions of parents have an impact on providing risky playing opportunities.
Pengembangan Lampu Warna terhadap Perkembangan Kognitif Anak Kelompok A di TK PGRI 1 Bancaran
Authors: Kholidiyah Nurul , Adhani Dwi Nurhayati , Fitroh Siti Fadjryana
Published: April 2020

Researchers used a development model Borg Gall. Steps to be taken include the research and data collection, planning, early product development, initial test, revise the initial product, the main field testing and revision of the final product. Research and development is carried out through four stages of a trial that is testing subject matter experts, instructional media expert testing, field trials beginning, and major field trials. The subject of research and development that children in group A or 4-5 years in kindergarten PGRI 1 Bangcaran totaling 21 children. The data collection technique using a questionnaire (questionnaire subject matter experts, instructional media expert questionnaire, and the questionnaire responses of children) and documentation. The results showed that the values obtained from the color light matter experts for86.11% with the title of "high", 76.67% of instructional media expert with the title of "high", the initial field trials 83.9%with the title of "high", and the main field trials 86.9%with the title of "high". Based on the results of research and development has been done can be concluded that the media color lights as stimulationability to recognize the concept of color in group A at TK PGRI 1 Bancaran fit for use
A Formação Integral na Educação Profissional Tecnológica: Alguns Apontamentos
Authors: Appio Célia , Ewald Izilene , Silva Valdelino
Published: April 2020

A formação humana é fundamental para a concepção e desempenho da prática pedagógica. Assim, a formação integral, enquanto concepção teórica pressupõe o desenvolvimento integrado do sujeito durante seu percurso formativo, ou seja, possui em sua proposta de ensino potencialidades que pretende desenvolver cognitivamente por meio da mescla das mais diversas áreas do conhecimento inter-relacionando-as. Assim sendo, correlaciona e busca transmitir com clareza as conexões existentes entre: desejo, subjetividade, emoções, socialização, entre outros aspectos que não se resume exclusivamente à formação e desenvolvimento do intelecto. Nesse sentido, o presente trabalho objetiva discutir a importância da formação integral na Educação Profissional e Tecnológica. Na elaboração do presente trabalho, foi realizada uma pesquisa bibliográfica e documental, buscando identificar e analisar as diferentes abordagens envolvendo o termo educação integral. Assim, fez-se necessário observar qual o destaque da educação integral nos documentos oficiais brasileiros no que diz respeito à Educação Profissional e Tecnológica.
Materiais Didáticos Acessíveis para Alunos Surdos: Respeito linguístico no ensino médio integrado em modelagem do vestuário no IFSC - Jaraguá do Sul
Authors: Marquetti Cristiane , De Cesaro Humberto
Published: April 2020

Este artigo é um recorte da pesquisa sobre materiais didáticos acessíveis para alunos surdos que tem como objetivo principal desenvolver tutoriais em Língua Brasileira de Sinais – LIBRAS, para o curso do ensino médio integrado ao Técnico de Modelagem do Vestuário. Também aborda a importância da produção de materiais em Língua de Sinais para o acesso, permanência e êxito dos alunos surdos. A proposta deste artigo é explanar os conceitos abordados na legislação e na literatura fazendo um comparativo com a realidade vivida pelas pessoas surdas. Assim, os autores que fazem parte dessa abordagem são, Quadros e Souza (2008), Libâneo (2005), Freire (1996), Góes (2012), Lodi e Lacerda (2014), Sassaki (2010), Silva (2011), Frigotto (2001), Araujo e Frigotto (2005). Partindo de uma explanação sobre educação profissional e tecnológica – EPT no Brasil e suas práticas, dissertamos sobre as pessoas com deficiência dentro deste contexto, com foco nas pessoas surdas.
Contra-bandos: percursos dos sujeitos do PROEJA mapeados através do memorial
Authors: De Souza Vinicius , Suhr Inge
Published: April 2020

A presente pesquisa de caráter qualitativo, por meio de pesquisa participante, intenta contra-bandear para o currículo as histórias de vida dos estudantes do Programa Nacional de Integração a Educação Profissional com a Educação Básica na Modalidade de Educação de Jovens e Adultos – PROEJA, do Instituto Federal Catarinense – Campus Camboriú. A proposta surge a partir da seguinte problematização: as vivências dos estudantes do PROEJA são levadas em consideração na organização do trabalho pedagógico nessa modalidade de ensino? Na tentativa de responder a essa questão nos propomos ao uso do memorial como ferramenta pedagógica que favoreça o resgate das memórias dos estudantes do PROEJA, por meio do desenvolvimento de uma sequência didática. Para atingir estes objetivos pretende-se contextualizar historicamente o atendimento a alunos da Educação de Jovens e Adultos no âmbito da educação profissional e tecnológica; além de descrever a importância do reconhecimento da história de vida dos estudantes do PROEJA para a formação integral. A pesquisa se encontra dentro do macroprojeto inclusão e diversidade em espaços formais e não formais de ensino na educação profissional e tecnológica, que faz parte da linha de pesquisa de práticas educativas em Educação Profissional e Tecnológica.
A Oferta da Educação Especial sob a Perspectiva da Educação Inclusiva na Educação Profissional e Tecnológica
Authors: Tillmann Luana , Almeida Judith
Published: April 2020

Educação Especial e a Educação Profissional e Tecnológica são modalidades de ensino que se articulam de forma incipiente. Neste artigo, temos o objetivo de refletir sobre a oferta do Atendimento Educacional Especializado na EPT. Para isso, faremos uma pesquisa bibliográfica (RAUEN, 2002) com abordagem qualitativa (BOGDAN; BIKLEN, 1995), analisando simultaneamente legislações e documentos oficiais, bem como, discursos de teóricos que discorrem sobre o processo de inclusão social e escolar (SASSAKI, 1999) (MANTOAN, 2003, 2006) (CARVALHO, 2005). Consideramos que a inclusão de estudantes público-alvo da Educação Especial na Educação Profissional e Tecnológica se apresenta como um direito a emancipação, estando pautada na promoção de acessibilidade e de equidade de condições.
Immune response of horses to inactivated African horse sickness vaccines
Authors: Rodriguez Marina , Joseph Sunitha , Pfeffer Martin , Raghavan Rekha , Wernery Ulrich
Published: April 2020

Background: African horse sickness (AHS) is a devastating disease of equids that has been known for centuries. Many equids mainly in sub-Saharan Africa still die every year from this viral disease. This has an enormous economic impact on the horse industry, notwithstanding the dedication of horse owners in the daily care of their animals. Currently, prevention and control of the disease are based on live attenuated vaccines and control vector arthropods.Results: A total of 29 horses kept in an isolated desert area were divided into 2 groups and subcutaneously (sc) vaccinated. In Group 1, a total of 18 horses divided into 9 subgroups of 2 horses each were individually immunised with AHS serotypes 1 to 9. Eleven horses in Group 2 were immunised with all 9 serotypes simultaneously with 2 different injections by the combination of vaccine 1 containing 5 serotypes (1, 4, 7, 8, 9) and vaccine 2 containing 4 serotypes (2, 3, 5, 6). The vaccination experiment lasted 12 months. Blood samples were periodically drawn for serum antibody tests using ELISA and VNT. After each vaccination, blood was collected during a period of 2 weeks for PCR and virus isolation. After the booster vaccination, 27 horses seroconverted to the inactivated vaccines, but 2 horses responded poorly, as measured by ELISA. In Group 1, ELISA and VN antibodies declined between 5 and 7 months post vaccination (pv). Twelve months later, the antibody level in most of the horses dropped to the negative range, but after the annual booster, all horses again seroconverted strongly, as early as 1 week pv. In Group 2, ELISA antibodies turned positive after the first booster, and VN antibodies started to appear for some serotypes after primary vaccination. After the booster vaccination, VN antibodies increased in a different pattern for each serotype. Antibodies remained high for 12 months and increased strongly after the annual booster in 78% of the horses. PCR and virus isolation were negative.Conclusions: Horses vaccinated with single serotypes need a booster after 6 months, while simultaneously immunised horses need one after 12 months. Due to the non-availability of a facility in the UAE, no challenge infection could be carried out.
WITHDRAWN: Secretion of IFN-γ by transgenic mammary epithelial cells in vitro reduced mastitis infection risk in goats
Authors: Liu Ying , Zhang Hongyan , Dong Shasha , Li Boyu , Ma Weiming , Ge Lijiang , Hu Zhiyong , Su Feng
Published: April 2020

Background: Mastitis results in great economic loss to the dairy goat industry. Many approaches have attempted to decrease the morbidity associated with this disease, and among these, transgenic strategy have been recognized as a potential approach. A previous mammalian study reports that interferon-gamma (IFN-γ) has potential anti-bacterial bioactivity against infection in vitro; however, its capacity in vivo is ambiguous. Results: In this study, we first constructed targeting and homologous recombination vectors (containing the IFN-γ gene) and then transferred the vectors into goat mammary gland epithelial cells (GMECs). Enzyme digestion and sequencing analysis indicated that the vectors used in this study were built correctly. Subsequently, monoclonal cells were selected using puromycin and the polymerase chain reaction (PCR) test indicated that IFN-γ was correctly inserted downstream of the casein promoter. Monoclonal cells were then assessed for reducible expression, and reverse transcriptase-PCR (RT-PCR) and Western blot tests confirmed that monoclonal cells could express IFN-γ. Finally, anti-bacterial capacity was evaluated using bacterial counts and flow cytometry analysis. Decreased bacterial counts and cell apoptosis rates in transgenic GMECs demonstrated that the secretion of IFN-γ could inhibit bacterial proliferation and reduce the risk of mammary infection in goats. Conclusions: IFN-γ gene transfection in goat mammary epithelial cells could inhibit bacterial proliferation and reduce the risk of mammary infection in goats.
An. gambiae s.l. exhibit high intensity pyrethroid resistance throughout Southern and Central Mali (2016-2018); PBO or next generation LLINs may provide greater control
Authors: Sovi Arthur , Keita Chitan , Sinaba Youssouf , Dicko Abdourhamane , Traore Ibrahim , Cisse Moussa BM , Koita Ousmane , Dengela Dereje , Flatley Cecilia , Bankineza Elie , Mihigo Jules , Belemvire Allison , Carlson Jenny , Fornadel Christen , Oxborough Richard Martin
Published: April 2020

Background: Millions of pyrethroid LLINs have been distributed in Mali during the past 20 years which, along with agricultural use, has increased the selection pressure on malaria vector populations. This study investigated pyrethroid resistance intensity and susceptible status of malaria vectors to alternative insecticides to guide choice of insecticides for LLINs and IRS for effective control of malaria vectors. Methods: For 3 years between 2016 and 2018, susceptibility testing was conducted annually in 14-16 sites covering southern and central Mali. Anopheles gambiae s.l. were collected from larval sites and adult mosquitoes exposed in WHO tube tests to diagnostic doses of bendiocarb (0.1%) and pirimiphos-methyl (0.25%). Resistance intensity tests were conducted using CDC bottle bioassays (2016-17) and WHO tube tests (2018) at 1 × , 2 × , 5 × , and 10 × the diagnostic concentration of permethrin, deltamethrin and alpha-cypermethrin. WHO tube tests were conducted with pre-exposure to the synergist PBO followed by permethrin or deltamethrin. Chlorfenapyr was tested in CDC bottle bioassays at 100µg active ingredient per bottle and clothianidin at 2% in WHO tube tests. PCR was performed to identify species within the An. gambiae complex. Results: In all sites An. gambiae s.l. showed high intensity resistance to permethrin and deltamethrin in CDC bottle bioassay tests in 2016 and 2017. In 2018, WHO intensity tests resulted in survivors at all sites for permethrin, deltamethrin and alpha-cypermethrin when tested at 10× the diagnostic dose. Across all sites mean mortality was 33.7% with permethrin (0.75%) compared with 71.8% when pre-exposed to PBO (4%), representing a 2.13 fold increase in mortality. A similar trend was recorded for deltamethrin. There was susceptibility to pirimiphos-methyl, chlorfenapyr and clothianidin in all surveyed sites, including current IRS sites in Mopti Region. An. coluzzii was the primary species in 4 of 6 regions. Conclusions: Widespread high intensity pyrethroid resistance was recorded during 2016-18 and is likely to compromise the effectiveness of pyrethroid LLINs in Mali. PBO or chlrofenapyr LLINs should provide improved control of An. gambiae s.l. Clothianidin and pirimiphos-methyl insecticides are currently being used for IRS as part of a rotation strategy based on susceptibility being confirmed in this study.
Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity
Authors: Shkodra-Pula Blerina , Kretzer Christian , Jordan Paul M. , Klemm Paul , Koeberle Andreas , Pretzel David , Banoglu Erden , Lorkowski Stefan , Wallert Maria , Höppener Stephanie , Stumpf Steffi , Vollrath Antje , Schubert Stephanie , Werz Oliver , Schubert Ulrich
Published: April 2020

Background: Dual inhibitors of the 5-lipoxygenase-activating protein (FLAP) and the microsomal prostaglandin E2 synthase-1 (mPGES-1) may exert better anti-inflammatory efficacy and lower risks of adverse effects versus non-steroidal anti-inflammatory drugs. Despite these advantages, many dual FLAP/mPGES-1 inhibitors are acidic lipophilic molecules with low solubility and strong tendency for plasma protein binding that limit their bioavailability and bioactivity. Here, we present the encapsulation of the dual FLAP/mPGES-1 inhibitor BRP-187 into the biocompatible polymers acetalated dextran (Acdex) and poly(lactic-co-glycolic acid) (PLGA) via nanoprecipitation. Results: The nanoparticles containing BRP-187 were prepared by the nanoprecipitation method and analyzed by dynamic light scattering regarding their hydrodynamic diameter, by scanning electron microscopy for morphology properties, and by UV-VIS spectroscopy for determination of the encapsulation efficiency of the drug. Moreover, we designed fluorescent BRP-187 particles, which showed high cellular uptake by leukocytes, as analyzed by flow cytometry. Finally, BRP-187 nanoparticles were tested in human polymorphonuclear leukocytes and macrophages to determine drug uptake, cytotoxicity, and efficiency to inhibit FLAP and mPGES-1.Conclusion: Our results demonstrate that encapsulation of BRP-187 into Acdex and PLGA is feasible, and both PLGA- and Acdex-based particles loaded with BRP-187 are more efficient in suppressing 5-lipooxygenase product formation and prostaglandin E2 biosynthesis in intact cells as compared to the free compound, particularly after prolonged preincubation periods.
Detection of gray matter microstructural changes in Alzheimer’s disease continuum using fiber orientation
Authors: Lee Peter , Kim Hang-Rai , Jeong Yong
Published: April 2020

There have been several magnetic resonance (MR) imaging biomarkers of Alzheimer’s disease (AD) for early diagnosis. Cortical mean diffusivity (MD) is one of them for the study of the cortical microstructural change in AD. However, the feasibility of MD often remains in doubt as partial volume effects may overestimate the results. This study aims to investigate feasible gray matter microstructural biomarker with higher sensitivity for early AD detection. We propose diffusion tensor imaging (DTI) measure, ‘radiality’, for early AD biomarker. It is a dot product between cortical surface normal vector and primary diffusion direction, which reflects the fiber orientation within the cortical column.
Here, we gathered neuroimages from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database: 78 cognitive normal, 50 early mild cognitive impairment (EMCI), 34 late mild cognitive impairment (LMCI), and 39 AD patients. Then, we evaluated cortical thickness (CTh), MD, amount of amyloid and tau accumulations using positron emission tomography (PET), which are conventional AD biomarkers. Radiality was projected on gray matter surface to compare and validate the changes along other neuroimage biomarkers.
Results showed decreased radiality primarily in entorhinal, insula, frontal and temporal cortex as disease progresses onward. Especially, radiality could delineate the difference between cognitive normal and EMCI group while other biomarkers could not. We looked into the relationship between the radiality and other biomarkers to validate its pathological evidence in AD. Overall, radiality showed high association with conventional biomarkers. Additional ROI analysis exhibits dynamics of AD related changes as stages onward.
In conclusion, radiality in cortical gray matter showed AD specific changes and relevance with other conventional AD biomarkers with higher sensitivity. Besides, it could show group differences in early AD changes from EMCI which show advantage for early diagnosis than using conventional biomarkers. We provide the evidence of structure changes with cognitive impairment and suggest radiality as a sensitive biomarker for early AD.
Association of glucose variability with postoperative delirium in acute aortic dissection patients: an observational study
Authors: Lin Yanjuan , Lin Ling-Yu , Peng Yan-Chun , Zhang Hao-Ruo , Chen Liang-wan , Huang Xi-Zhen , Chen Qiong
Published: April 2020

Background

Blood glucose variability is associated with poor prognosis after cardiac surgery, but the relationship between glucose variability and postoperative delirium in patients with acute aortic dissection is unclear. The aim of this study is to investigate the association of blood glucose variability with postoperative delirium in acute aortic dissection patients.
Methods

We prospectively analyzed 257 patients including 103 patients with delirium. The patients was categorized into two groups according to whether delirium was present. The outcome measures were postoperative delirium, the length of Intensive Care Unit stay and the duration of hospital stay. Multivariable Cox competing risk survival models was used to assess.
Results

A total of 257 subjects were enrolled, including 103 patients with delirium. There were statistically significant differences between the two groups in age, body mass index, first admission blood glucose, white blood cell counts, Acute Physiology and Chronic Health Evaluation II score, hypoxemia, mechanical ventilation duration and the length of Intensive Care Unit stay (P < 0.05). The median of mean of blood glucose and standard deviation of blood glucose were higher in the delirium group than in the non-delirium group, and the difference was statistically significant (P < 0.05). In model 1, the adjusted hazard ratio of standard deviation of blood glucose was 1.436 (P < 0.05). In Model 2, the SDBG (AHR = 1.418, 95% CI = 1.195–1.681, P < 0.05) remained significant after adjusting for confounders (P < 0.05). The area under curve of the SDBG ROC was 0.763 (95% CI = 0.704–0.821, P < 0.01). The sensitivity was 81.6%, and the specificity was 57.8%.
Conclusions

Glucose variability is associated with the risk of delirium in patients after aortic dissection surgery, and high glycemic variability increases the risk of postoperative delirium.
Supporting rural citizens to participate in healthcare activities and interprofessional collaboration: Effects of an intervention in Japanese communities
Authors: Ohta Ryuichi , Ryu Yoshinori , Kitayuguchi Jun , Gomi Tetsunosuke , Katsube Takuji , Könings Karen D
Published: April 2020

Background To receive high-quality healthcare, citizens should actively participate in public healthcare programs and share decision-making with medical professionals, especially in rural areas; this requires support for citizens in the form of education. Few studies examine educational interventions’ effects on rural citizens’ motivation to participate in public healthcare efforts or interprofessional collaboration (IPC) perceptions. Thus, the current study investigates rural citizens’ participation and difficulties faced in healthcare efforts and educational intervention effects.Methods As a quasi-experimental, mixed-methods study, 277 (156=intervention group; 121=control group) rural Japanese participants were surveyed before and after a healthcare workshop, and scores were compared. Semi-structured interviews were also conducted with 17 participants post-workshop. The contents of the interviewed were analyzed based on the framework of thematic analysis.Results Regarding pretest–posttest difference, intervention-group participants scored significantly higher regarding participating in planning and managing self-care than control-group participants (p=0.001 and p=0.014, respectively); there was no difference regarding having a dialogue with healthcare staff and sharing knowledge. Interviews revealed three themes: ability to manage health conditions, relationship with medical professionals, and relationship among citizens. Participants reported difficulties in judging symptoms and communicating with medical professionals. Hierarchy and citizens’ low motivation to participate in IPC hindered collaboration. Furthermore, community conditions, including weakening relationships, privacy issues, and social norms, affected healthcare behaviors. Conclusions Educational interventions emphasizing healthcare and IPC may positively impact rural citizens’ healthcare perceptions. However, citizens may experience difficulties changing their community conditions, enhancing limited healthcare knowledge/skills, and maintaining good relationships with medical professionals and other citizens. Both continuous educational information and interventions and dialogue among medical professionals and rural citizens should be promoted.
Reducing Veterans' Risk for Suicidal Behaviors: A Qualitative Study to Inform Development of the RECLAIM Health Promotion Program
Authors: Shue Sarah , Brosmer Jayme , Matthias Marianne
Published: April 2020

Background In an effort to reduce the high rate of suicide among post-9/11 veterans, a collaborative team within the Department of Veterans Affairs (VA) has developed a holistic community-based health promotion program designed to facilitate social and self-connectedness. The purpose of this study was to elicit veteran and stakeholder feedback to prepare the program for piloting and implementation.Methods Focus groups and interviews were conducted with post-9/11 veterans and veteran stakeholders (e.g., VA clinicians) to elicit feedback regarding the health promotion program. Focus groups and interviews were audio-recorded and transcribed. Qualitative thematic analysis identified key themes emerging from the focus groups and interviews.Results Seven focus groups (3 Veteran groups, 4 stakeholder groups) and 3 interviews (2 Veterans, 1 stakeholder) were conducted with 41 participants (14 veterans, 27 stakeholders). Overall, participants had a positive perception of the program. Thematic analysis revealed shared perspectives that provided insight into 1) enhancing program recruitment and retention, 2) the perceived ability of a health promotion program to provide more holistic, veteran-centered care, and 3) using health promotion programs to help veterans establish structure in their daily lives.Conclusions Findings indicated an overall acceptance of the program, and participants’ perspectives on how to reduce barriers and enhance facilitators can inform the development of a larger-scale health promotion program that can be tested through future research. While discussion questions were specifically focused on the program in this study, findings can be considered more broadly for the design and implementation of related programs to effectively improve the health and wellness of post-9/11 veterans.
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines
Authors: Hansen Tom Johnny , Penman David , Glover Kevin Alan , Fraser Thomas , Vågseth Tone , Thorsen Anders , Sørvik Anne Grete Eide , Fjelldal Per Gunnar
Published: April 2020

Background

In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits. The Atlantic salmon is one of the best studied fish species globally, but all experiments are done on fish of wild or domesticated origin and access to standardized immortal fish lines would be of great benefit. Here, we describe the protocols developed to produce mitotic gynogenes, and from these the first clonal lines in Atlantic salmon.
Results

Atlantic salmon eggs fertilized with UV irradiated sperm combined with a pressure shock applied at 4700–4800 minC at 8 oC gave all homozygous (doubled haploid) gynogenetic progeny with high survival. From the six first maturing females, five all homozygous clonal lines were produced by meiotic gynogenesis and were verified as clonal and identical to their mother with microsatellite markers.
Conclusions

We have now produced the first documented cloned Atlantic salmon lines. This work demonstrates the potential for production of further Atlantic salmon clonal lines, potentially with distinct characteristics. Such lines will provide an important resource for further elucidation of phenotypic and genetic traits in this globally important species.
Elevated TG/HDL-C and Non-HDL-C/HDL-C Ratios Predict Mortality in Peritoneal Dialysis Patients
Authors: Xia Wenkai , Yao Xiajuan , Chen Yan , Lin Jie , Vielhauer Volker , Hu Hong
Published: April 2020

Background and Aims:
Dyslipidemia is common in patients with chronic kidney disease and particular prevalent in patients receiving peritoneal dialysis. However, whether markers of atherogenic dyslipidemia correlate with outcomes in dialysis patients as in the general population is uncertain. Here, we investigated the prognostic value of the serum triglyceride/HDL cholesterol (TG/HDL-C) ratio and non-HDL-C/HDL-C ratio in peritoneal dialysis patients to predict all-cause mortality.
Methods
214 PD patients were retrospectively analyzed from January 2011 to December 2015, with a median follow-up of 59 months. We used receiver operating curves (ROC) to determine the optimal threshold for TG/HDL-C and non-HDL/HDL-C ratios at baseline to predict OS during follow-up. Prognostic values were accessed by univariate and multivariate COX regression analysis and Kaplan-Meier curve. A predictive nomogram was developed to predict prognosis for overall survival, and the predictive accuracy was evaluated by concordance index (c-index).
Results
The optimal cut-off values for TG/HDL-C ratio and non-HDL-C/HDL-C ratio were 1.94 and 2.86, respectively. A high TG/HDL-C ratio and a high non-HDL-C/HDL-C ratio strongly correlated with worse OS in PD patients. Multivariate analysis demonstrated that elevated TG/HDL-C ratio as well as non-HDL/HDL-C ratios were independent markers to predict reduced OS. The TG/HDL-C ratio (HR 2.60, 95% CI 1.40–4.83, P = 0.002) was superior to non-HDL-C/HDL-C ratio based on hazard ratio (HR 2.43, 95% CI 1.09–5.40, P = 0.029).
Conclusion
TG/HDL-C ratio and non-HDL-C/HDL-C were identified as potential prognostic biomarkers in PD patients. The proposed nomograms can be utilized for prediction of OS in PD patients.