bluuebunny/crossref_metadata_2025 huggingface dataset:
A design method of volute profile of multi-blade centrifugal fan based on DRBF model
Authors: Zhang A. Tianle , Zhou B. Shuiqing , Mao C. Zijian , He D. Jiacheng , Fang E. Xiaohai
Published: December 2023

The forward-curved multi-blade centrifugal fan is widely used in the ventilation and household appliance industries with limited space due to its small size factor, high pressure coefficient, and large outlet flow coefficient. Conventional centrifugal fan structural designs rely on empirical data, resulting in drawbacks during usage such as low airflow velocity, high noise levels, and high energy consumption. In order to optimize fan structure, enhance aerodynamic performance, and seek new, effective approaches for energy efficiency and emissions reduction, this paper focuses on a combined design method that integrates scroll line design with aerodynamic performance and noise control. The goal is to propose a noise prediction and analysis method for multi-blade centrifugal fans, addressing the design contradiction between optimal fan functionality and minimal noise within limited design dimensions. The dynamic radial basis function method is introduced to investigate the geometric parameters of the scroll line, utilizing coherence analysis to identify the reasons for insufficient aerodynamic performance and noise reduction measures. Through the research, it is found that unreasonable design leads to a large turbulent vortex region within the blade passage and internal flow field, particularly involving circulation and backflow, which significantly reduces the fan's aerodynamic performance and operational efficiency. The design incorporates a composite scroll with a logarithmic spiral and circular arc integrated into one, and algorithm-based optimization of the scroll tongue, facilitating the transition of turbulence at the scroll outlet and the gap between the scroll tongue and impeller. Numerical results show an increase in airflow of 3.04 m3/min and a noise reduction of 3.79%. This indicates that the composite scroll design with a logarithmic spiral plays a dominant role in accelerating the aerodynamic performance of the scroll and reducing noise radiation.
An augmented invariant-based model of the pressure Hessian tensor using a combination of physics-assisted neural networks
Authors: Shikha Deep , Sinha Sawan S.
Published: December 2023

Modeling the velocity gradient dynamics in incompressible turbulence requires modeling two unclosed quantities: the pressure Hessian tensor and the viscous Laplacian tensor. In this work, we model the pressure Hessian tensor using a combination of two different physics-embedded deep neural networks. The first neural network is trained specifically to predict the alignment tendencies of the eigen-vectors of the pressure Hessian tensor, whereas the second neural network is trained only to predict the magnitude of the tensor. This separation of tasks allows us to define mathematically optimal and physics-informed customized loss functions separately for the two aspects (alignment and magnitude) of the tensor. Both neural networks take invariants of the velocity gradient tensor as inputs. Even though the training of the two networks is performed using direct numerical simulation database of an incompressible stationary isotropic turbulence at a particular Reynolds number, we extensively evaluate the model at different Reynolds numbers and in different kinds of flow fields. In incompressible flows, the proposed model shows significant improvements over the existing phenomenological model (the recent fluid deformation closure model or the RFD model) of the pressure Hessian tensor. While the improvements in the alignment tendencies are convincingly evident in the shapes of the probability density functions of the cosines of various angles between eigenvectors, the improvements in the prediction of the magnitude of the pressure Hessian tensor using the new model are quantifiable in the range of 28%–89% (depending on the type of the flow field) compared to the RFD model.
Optical Nonlinearity of Violet Phosphorus and Applications in Fiber Lasers
Authors: Yang 杨 Hui-ran 慧苒 , Qi 齐 Meng-ting 梦婷 , Li 李 Xu-peng 旭鹏 , Xue 薛 Ze 泽 , Lu 鲁 Chen-hao 晨浩 , Cheng 成 Jia-wei 嘉伟 , Han 韩 Dong-dong 冬冬 , Li 李 Lu 璐
Published: December 2023

A D-shaped fiber is coated with a new two-dimensional nanomaterial, violet phosphorus (VP), to create a saturable absorber (SA) with a modulation depth of 3.68%. Subsequently, the SA is inserted into a fiber laser, enabling successful generation of dark solitons and bright–dark soliton pairs through adjustment of the polarization state within the cavity. Through further study, mode-locked pulses are achieved, proving the existence of polarization-locked vector solitons. The results indicate that VP can be used as a polarization-independent SA.
Predicted Critical State Based on Invariance of the Lyapunov Exponent in Dual Spaces
Authors: Liu 刘 Tong 通 , Xia 夏 Xu 旭
Published: December 2023

Critical states in disordered systems, fascinating and subtle eigenstates, have attracted a lot of research interests. However, the nature of critical states is difficult to describe quantitatively, and in general, it cannot predict a system that hosts the critical state. We propose an explicit criterion whereby the Lyapunov exponent of the critical state should be 0 simultaneously in dual spaces, namely the Lyapunov exponent remains invariant under the Fourier transform. With this criterion, we can exactly predict a one-dimensional quasiperiodic model which is not of self-duality, but hosts a large number of critical states. Then, we perform numerical verification of the theoretical prediction and display the self-similarity of the critical state. Due to computational complexity, calculations are not performed for higher dimensional models. However, since the description of extended and localized states by the Lyapunov exponent is universal and dimensionless, utilizing the Lyapunov exponent of dual spaces to describe critical states should also be universal. Finally, we conjecture that some kind of connection exists between the invariance of the Lyapunov exponent and conformal invariance, which can promote the research of critical phenomena.
Enhancement and switch effect of equal-spin Andreev reflection in ferromagnet/insulator/Ising superconductor junctions
Authors: Li Wen , Yang De-Jing , Lu Wei-Tao
Published: December 2023

We study the property of equal-spin Andreev reflection (ESAR) in the ferromagnet/insulator/Ising superconductor junction where Ising spin–orbit coupling is taken into account in the insulator. It is found that the ESAR exhibits a regular oscillation with the insulating barrier, the amplitude and period of which can be effectively controlled by the chemical potentials. Compared to that in the ferromagnet/Ising superconductor junction, the ESAR is greatly increased due to the resonant mode, suggesting an enhanced spin-triplet pairing. As an application, the proposed junction may work as a switch to turn on and off the ESAR. Furthermore, the insulating barrier does not change the magnetoanisotropic period of ESAR because of the invariant symmetry of the system, however, the magnetoanisotropy is strengthened.
First-principles study of phase transition in the α-In2Se3/metal heterostructures
Authors: Yu Zhuo-Liang , Zhang Chen-Ye , Tan Qing-Shou , Tao Jia-You , Sun Xiao-Xiang , Luo Liang , Li Fen , Zhao Yu-Qing , Li Chang , Lang Lin
Published: December 2023

With the discovery of two-dimensional (2D) ferroelectric materials such as CuInP2S6 and α-In2Se3, the ferroelectric field effect transistors (Fe-FETs) based on these materials have entered a rapid-development period. The metal/semiconductor contact is an unavoidable topic in the construction of devices. In this paper, heterostructures α-In2Se3/metals (Pd, Pt, Cu, Ag and Au) are discussed. According to different stacking types, the structures and energy of 160 heterostructures are calculated and compared. When α-In2Se3 contacts with the Pd, Pt and Cu, the α-In2Se3 may transforms into β-In2Se3. This phenomenon has hardly been mentioned or analyzed in previous reports. Contacting with the Au and Ag, the α-In2Se3 maintains the original structure. The internal physical mechanism of phase transition is explained from the binding energy and the charge transfer. The paper provides sufficient theoretical support for research and development of the Fe-FETs based on α-In2Se3.
An efficient diagnostic strategy for intermittent faults in electronic circuit systems by enhancing and locating local features of faults
Authors: Jia Zhen , Wang Shengdong , Zhao Ke , Li Zhifei , Yang Qiqi , Liu Zhenbao
Published: December 2023

Due to their short duration, concealability, and random occurrence, intermittent faults have become the most dangerous hazard in electronic circuit systems. However, existing intelligent diagnostic methods often struggle to provide substantial effectiveness for such objects. This article proposes a scheme to enhance the output signal of electronic circuits to make the features more intuitive and facilitate fault feature mining, and designs a scheme to mine tiny fault features from global signals. First, the circuit’s output signal undergoes an S-transform to obtain its time-frequency domain characteristics. Next, a sequence and excitation networks attention module is employed to allocate weights to different channels. Finally, the aforementioned output is used as input to the Swin transformer framework to thoroughly explore fault features. Three electronic circuits are used as experimental circuits to test the proposed method. The experiment shows that the proposed diagnostic method is fast and has an accuracy of over 97%. Therefore, the effectiveness of the designed strategy that includes multiple attention mechanisms in mining intermittent fault features in electronic circuit systems has been demonstrated.
A novel pulse-echo piezoelectric transducer for detecting quasi-static component induced by an ultrasonic longitudinal wave
Authors: Lai Quanqing , Lu Lu , Xu Caibin , Hu Ning , Deng Mingxi
Published: December 2023

For effectively detecting the quasi-static component (QSC) of an ultrasonic longitudinal wave, which is closely related to the elastic nonlinearity of material, we proposed a novel pulse-echo piezoelectric transducer consisting of a high-frequency piezoelectric wafer, a frequency selective isolation layer, a low-frequency piezoelectric wafer, and an acoustic backing. The high-frequency wafer generates the primary longitudinal wave (PLW) tone burst, while the high- and low-frequency wafers receive the pulse echo containing both the PLW component and QSC, respectively. We analyze the pulse-echo formation of the high-frequency PLW tone burst in a specimen, and conduct numerical simulations and experiments to validate the effectiveness of the proposed transducer. The results demonstrate that the low-frequency receiver is more efficient at detecting the QSC, even though the high-frequency wafer can also receive the echo of the QSC pulse. Specifically, the QSC pulse can still be detected by the low-frequency receiver when the high-frequency PLW tone burst is completely attenuated. The novel pulse-echo transducer proposed in this paper expands the design perspectives for transducers used in ultrasonic non-destructive testing.
A 3D transfer learning approach for identifying multiple simultaneous errors during radiotherapy
Authors: van den Berg Kars , Wolfs Cecile J A , Verhaegen Frank
Published: December 2023

Objective. Deep learning models, such as convolutional neural networks (CNNs), can take full dose comparison images as input and have shown promising results for error identification during treatment. Clinically, complex scenarios should be considered, with the risk of multiple anatomical and/or mechanical errors occurring simultaneously during treatment. The purpose of this study was to evaluate the capability of CNN-based error identification in this more complex scenario. Approach. For 40 lung cancer patients, clinically realistic ranges of combinations of various treatment errors within treatment plans and/or computed tomography (CT) images were simulated. Modified CT images and treatment plans were used to predict 2580 3D dose distributions, which were compared to dose distributions without errors using various gamma analysis criteria and relative dose difference as dose comparison methods. A 3D CNN capable of multilabel classification was trained to identify treatment errors at two classification levels, using dose comparison volumes as input: Level 1 (main error type, e.g. anatomical change, mechanical error) and Level 2 (error subtype, e.g. tumor regression, patient rotation). For training the CNNs, a transfer learning approach was employed. An ensemble model was also evaluated, which consisted of three separate CNNs each taking a region of interest of the dose comparison volume as input. Model performance was evaluated by calculating sample F1-scores for training and validation sets. Main results. The model had high F1-scores for Level 1 classification, but performance for Level 2 was lower, and overfitting became more apparent. Using relative dose difference instead of gamma volumes as input improved performance for Level 2 classification, whereas using an ensemble model additionally reduced overfitting. The models obtained F1-scores of 0.86 and 0.62 on an independent test set for Level 1 and Level 2, respectively. Significance. This study shows that it is possible to identify multiple errors occurring simultaneously in 3D dose verification data.
On dosimetric characteristics of detectors for relative dosimetry in small fields: a multicenter experimental study
Authors: Casar Božidar , Mendez Ignasi , Gershkevitsh Eduard , Wegener Sonja , Jaffray David , Heaton Robert , Pesznyak Csilla , Stelczer Gabor , Bulski Wojciech , Chełminski Krzysztof , Smirnov Georgiy , Antipina Natalia , Beavis Andrew W , Harding Nicholas , Jurković Slaven , Hwang Min-Sig , Saiful Huq M
Published: December 2023

Objective. In this multicentric collaborative study, we aimed to verify whether the selected radiation detectors satisfy the requirements of TRS-483 Code of Practice for relative small field dosimetry in megavoltage photon beams used in radiotherapy, by investigating four dosimetric characteristics. Furthermore, we intended to analyze and complement the recommendations given in TRS-483. Approach. Short-term stability, dose linearity, dose-rate dependence, and leakage were determined for 17 models of detectors considered suitable for small field dosimetry. Altogether, 47 detectors were used in this study across ten institutions. Photon beams with 6 and 10 MV, with and without flattening filters, generated by Elekta Versa HDTM or Varian TrueBeamTM linear accelerators, were used. Main results. The tolerance level of 0.1% for stability was fulfilled by 70% of the data points. For the determination of dose linearity, two methods were considered. Results from the use of a stricter method show that the guideline of 0.1% for dose linearity is not attainable for most of the detectors used in the study. Following the second approach (squared Pearson’s correlation coefficient r
2), it was found that 100% of the data fulfill the criteria r
2 > 0.999 (0.1% guideline for tolerance). Less than 50% of all data points satisfied the published tolerance of 0.1% for dose-rate dependence. Almost all data points (98.2%) satisfied the 0.1% criterion for leakage. Significance. For short-term stability (repeatability), it was found that the 0.1% guideline could not be met. Therefore, a less rigorous criterion of 0.25% is proposed. For dose linearity, our recommendation is to adopt a simple and clear methodology and to define an achievable tolerance based on the experimental data. For dose-rate dependence, a realistic criterion of 1% is proposed instead of the present 0.1%. Agreement was found with published guidelines for background signal (leakage).
A versatile semi-active magnetorheological inerter with energy harvesting and active control capabilities
Authors: Cao Jing , Ning Donghong , Liu Pengfei , Sun Shuaishuai , Liu Guijie , Du Haiping
Published: December 2023

Semi-active devices typically adjust the system’s damping coefficient to control vibration, offering advantages such as excellent performance and low power consumption. However, the output force of the traditional variable damping (VD) device can only be opposite to the relative motion direction of the device’s two terminals, which limits the vibration control performance. This paper introduces a versatile semi-active magnetorheological (MR) inerter with three operating modes, the VD, energy harvesting, and active control modes, to break through the performance bottleneck of traditional semi-active devices. The MR inerter combines two MR dampers and a flywheel, acting as the controllable units and energy sink. The built prototype is tested, and its parameters are identified. When the innovative semi-active inerter works with a corresponding control strategy to regulate the current in two MR dampers, it can achieve vibration energy storage and release. The harvested energy can help to reduce the high dependency of the semi-active output force on external inputs. The proposed semi-active inerter has excellent potential in the future applications.
Demonstrating more than 2π phase modulation in non-Hermitian asymmetric multilayers
Authors: Simone Giuseppina
Published: December 2023

Phase modulation has come to be recognized as a fundamental paradigm for optical device design in applications involving the spatiotemporal control of optical wavefronts. Here, the cavities use a combination of absorptive and lossless layers with ideal conductive metal to control reflection and phase shift, similar to the Fabry–Pérot system, which has zero reflection and perfect transmission. Multilayers with different sequences and composed of several stack layers were examined for reflectivity when activated in a prism-coupled layout. The layer’s different arrangements, resulting in open and closed asymmetric multilayers, display reflectance spectra with distinct features as well as phase and shifts. In the open configuration, there is a strong coupling between the hybridized resonance mode and the exciton from the red dye. As a result, energy-based topological considerations identify a correlation between exciton/polariton strong coupling, topological darkness, and phase tuning, and they motivate the

3
π

phase shift range.
Convergence of digitized-counterdiabatic QAOA: circuit depth versus free parameters
Authors: Vizzuso Mara , Passarelli Gianluca , Cantele Giovanni , Lucignano Procolo
Published: December 2023

Recently, digitized-counterdiabatic (CD) quantum approximate optimization algorithm (QAOA) has been proposed to make QAOA converge to the solution of an optimization problem in fewer steps, inspired by Trotterized CD driving in continuous-time quantum annealing. In this paper, we critically revisit this approach by focusing on the paradigmatic weighted and unweighted one-dimensional MaxCut problem. We study two variants of QAOA with first and second-order CD corrections. Our results show that, indeed, higher order CD corrections allow for a quicker convergence to the exact solution of the problem at hand by increasing the complexity of the variational cost function. Remarkably, however, the total number of free parameters needed to achieve this result is independent of the particular QAOA variant analyzed for the problems considered.
The electron–phonon scattering and charge transport of the two-dimensional (2D) polar h-BX(X = P, As, Sb) monolayers
Authors: Zhang Xiaomei , Li Huike , Ma Xin , Zhou Xiaohui , Zheng Zhongtian , Shen Chenhai , Xia Congxin , Liu Yufang
Published: December 2023

In this paper, the electron–phonon scattering and phonon-limited transport properties of the two-dimensional polar h-BX(X = P, As, Sb) have been studied through first-principles calculations in combination with Boltzmann transport theory. The electron–phonon scattering in these three systems is systematically assessed. Remarkably, intravalley scattering and intervalley scattering are separately investigated, of which the contribution to total scattering is found to be relatively comparable. The carrier mobility is determined over a broad range of carrier concentrations. The results indicate that h-BX (BP, BAs, BSb) simultaneously possess ultrahigh electron mobilities (4097 cm2 V−1 s−1, 4141 cm2 V−1 s−1, 12 215 cm2 V−1 s−1) and hole mobilities (7563 cm2 V−1 s−1, 7606 cm2 V−1 s−1, 22 282 cm2 V−1 s−1) at room temperature as compared to the most known two-dimensional (2D) materials. Additionally, it is discovered that compressive strain can induce a further increase in carrier mobility. The exceptional charge transport properties exhibited by these 2D semiconductors are attributed to the small effective masses in combination with the significant suppression of scattering due to high optical longitudinal optical- and transverse optical-phonon frequencies. This is the first time that we have provided a systematic interpretation of the reason for the exceptional charge transport properties exhibited by the 2D h-BX(X = P, As, Sb) semiconductors. Our finding can provide a theoretical perspective regarding the search for 2D materials with the high carrier mobility.
Exact moments and re-entrant transitions in the inertial dynamics of active Brownian particles
Authors: Patel Manish , Chaudhuri Debasish
Published: December 2023

In this study, we investigate the behavior of free inertial active Brownian particles in the presence of thermal noise. While finding a closed-form solution for the joint distribution of positions, orientations, and velocities using the Fokker–Planck equation is generally challenging, we utilize a Laplace transform method to obtain the exact temporal evolution of all dynamical moments in arbitrary dimensions. Our expressions in d dimensions reveal that inertia significantly impacts steady-state kinetic temperature and swim pressure while leaving the late-time diffusivity unchanged. Notably, as a function of activity and inertia, the steady-state velocity distribution exhibits a remarkable re-entrant crossover from ‘passive’ Gaussian to ‘active’ non-Gaussian behaviors. We construct a corresponding ‘phase diagram’ using the exact expression of the d-dimensional kurtosis. Our analytic expressions describe steady states and offer insights into time-dependent crossovers observed in moments of velocity and displacement. Our calculations can be extended to predict up to second-order moments for run-and-tumble particles and the active Ornstein–Uhlenbeck process (AOUP). Additionally, the kurtosis shows differences from AOUP.
Characterizing the spontaneous collapse of a wavefunction through entropy production
Authors: Artini Simone , Paternostro Mauro
Published: December 2023

We investigate the phenomenology leading to the non-conservation of energy of the continuous spontaneous localization (CSL) model from the viewpoint of non-equilibrium thermodynamics, and use such framework to assess the equilibration process entailed by the dissipative formulation of the model (dCSL). As a paradigmatic situation currently addressed in frontier experiments aimed at investigating possible collapse theories, we consider a one-dimensional mechanical oscillator in a thermal state. We perform our analysis in the phase space of the oscillator, where the entropy production rate, a non-equilibrium quantity used to characterize irreversibility, can be conveniently analyzed. We show that the CSL model violates Clausius law, as it exhibits a negative entropy production rate, while the dCSL model reaches equilibrium consistently only under certain dynamical conditions, thus allowing us to identify the values—in the parameter space—where the latter mechanism can be faithfully used to describe a thermodynamically consistent phenomenon.
Multiscale numerical study of the ELM-induced sputtering on the tungsten castellated divertor gap
Authors: Niu Guo-jian , Kawamura Gakushi , Dai Shu-yu , Yang Zhong-shi , Ding Fang , Xu Qian , Wang Rong , He Tao , Luo Guang-Nan
Published: December 2023

Material sputtering and erosion are key issue in fusion science. In the steady state, tungsten sputtering can be maintained at a low level. However, tungsten is seriously sputtered by the hot plasma from edge localized mode (ELM) bursts. The time evolution and spatial distribution of sputtering on the divertor gap are still not clear. This unclearness influences the understanding of ELM-induced sputtering and the explanation of experimental phenomenon. In this study, the evolution of tungsten flux generated by ELM burst is obtained via hybrid multiscale simulation and a double-peak of tungsten flux is found. The first peak is produced by the ELM electrons, which provides great sheath potential, and the second peak is generated by the energetic ions from ELM. The castellated divertor gap (CDG) can influence the tungsten sputtering distribution due to the change of the impacting angle. The sputtering and erosion on CDG are more serious than that on the mono-block top, but the tungsten source from the gap is very insignificant because the gap size is small. The simulation results are compared with EAST experiments and a qualitative consistency is obtained.
Device comparison study to measure nasal nitric oxide in relation to primary ciliary dyskinesia
Authors: Jõgi Nils Oskar , Ersson Karin , Alving Kjell , Krantz Christina , Malinovschi Andrei
Published: December 2023

Primary ciliary dyskinesia (PCD) is a genetic respiratory disease characterized by chronic cough, recurrent respiratory infections, and rhinosinusitis. The measurement of nasal nitric oxide (nNO) against resistance has been suggested as a sensitive screening method. However, current recommendations argue for the use of expensive, chemiluminescence devices to measure nNO. This study aimed to compare nNO measurement using three different devices in distinguishing PCD patients from healthy controls and cystic fibrosis (CF) patients and to evaluate their diagnostic precision. The study included 16 controls, 16 PCD patients, and 12 CF patients matched for age and sex. nNO measurements were performed using a chemiluminescence device (Eco Medics CLD 88sp), and two devices based on electrochemical sensors (Medisoft FeNO+ and NIOX Vero) following standardized guidelines. Correlation estimation, Bland–Altman, ROC curve, and one-way ANOVA were used to assess device differences and diagnostic performance. Significantly lower nNO output values were observed in PCD and CF patients compared to controls during exhalation against resistance. The correlation analysis showed high agreement among the three devices. ROC curve analysis demonstrated 100% sensitivity and specificity at different cut-off values for all devices in distinguishing PCD patients from controls (optimal cut-offs: EcoMedics 73, Medisoft 92 and NIOX 87 (nl min−1 )). Higher nNO output values were obtained with the Medisoft and NIOX devices as compared to the EcoMedics device, with a bias of−19 nl min−1 (95% CI: −73–35) and −21 nl min−1 (−73–31) accordingly. These findings indicate that all three tested devices can potentially serve as diagnostic tools for PCD if device specific cut-off values are used. This last-mentioned aspect warrants further studies and consideration in defining optimal cut-offs for individual device.
Direct 4D printing of functionally graded hydrogel networks for biodegradable, untethered, and multimorphic soft robots
Authors: Cho Soo Young , Ho Dong Hae , Jo Sae Byeok , Cho Jeong Ho
Published: December 2023

Recent advances in functionally graded additive manufacturing (FGAM) technology have enabled the seamless hybridization of multiple functionalities in a single structure. Soft robotics can become one of the largest beneficiaries of these advances, through the design of a facile four-dimensional (4D) FGAM process that can grant an intelligent stimuli-responsive mechanical functionality to the printed objects. Herein, we present a simple binder jetting approach for the 4D printing of functionally graded porous multi-materials (FGMM) by introducing rationally designed graded multiphase feeder beds. Compositionally graded cross-linking agents gradually form stable porous network structures within aqueous polymer particles, enabling programmable hygroscopic deformation without complex mechanical designs. Furthermore, a systematic bed design incorporating additional functional agents enables a multi-stimuli-responsive and untethered soft robot with stark stimulus selectivity. The biodegradability of the proposed 4D-printed soft robot further ensures the sustainability of our approach, with immediate degradation rates of 96.6% within 72 h. The proposed 4D printing concept for FGMMs can create new opportunities for intelligent and sustainable additive manufacturing in soft robotics.
Cardiogenic shock after 5-fluorouracil administration: a case report and literature review
Authors: Vanoverbeke Lowie , Wouter Holvoet , François D’Heygere , Ivan Elegeert
Published: December 2023

Background

Cardiogenic shock is a rare adverse event of 5-fluorouracil (5-FU) administration. Because of its rare entity, little is known about epidemiologic and clinical features of 5-FU-induced cardiogenic shock, and recommendations about specific treatment are missing.

Case summary

We present a case of cardiogenic shock and ventricular arrhythmia due to 5-FU-induced toxic cardiomyopathy treated with vasopressor and inotropic drugs in combination with intra-aortic balloon pump. Because of persistent haemodynamic instability, veno-arterial extracorporeal membrane oxygenation (VA-ECMO) and Impella were implanted as a bridge to recovery. Systolic function recovered completely and the patient was weaned successfully.

Discussion

This case demonstrates toxic cardiomyopathy as a rare and potentially lethal cardiac adverse event of 5-FU administration. This case emphasizes the importance of mechanical support as bridging therapy to recovery of cardiac function.
Safer spaces in youth development programs and health in Canadian youth
Authors: Ramey Heather L , Lawford Heather L , Berardini Yana , Mahdy Sharif S , Khanna Nishad , Ross Madeleine D , von Hugo Tonia K
Published: December 2023

Engagement in youth programs is a potential means to promote health and well-being across populations of young people. Safer spaces in these youth programs are likely critical in fostering positive health outcomes, but current research on the links between safer spaces and health is limited. In this exploratory study, we examined links between program safety in youth development programs and minoritized status, and health-related quality of life (HRQoL) and psychosomatic health complaints. Participants (N = 282; Mean age = 16.97 years; SD = 2.97) self-identified across various minority status groups, including LGBTQ (30%) and a range of perceived income levels. We tested a statistical model in which safer spaces, LGBTQ status and perceived income predicted HRQoL and health complaints in youth development program participants. LGBTQ status and lower perceived income were related to lower HRQoL and more health complaints, and safer space in youth development programs was related to better HRQoL. We also found an interaction effect, such that safer spaces in youth programs appeared to be especially beneficial for HRQoL for youth with higher incomes. Findings reinforce past research on LGBTQ status and income as factors for youth wellness and mental health. Findings also suggest that perceived safer spaces in youth development programs support better HRQoL and lower health complaints, across populations of participating youth.
Setting the scene: a scoping review of gambling research in Ghana
Authors: Badu Emmanuel , Hallett Jonathan , Vujcich Daniel , Crawford Gemma , Bellringer Maria E
Published: December 2023

Ghana, like many other African countries, has seen a rapid expansion in gambling industry activity in the last decade with sports betting becoming a popular pastime among young Ghanaian males. The proliferation of land based, and online gambling operations presents a significant public health threat to the citizenry. Yet little is known about gambling participation and harms, the size of the gambling market and the role of the gambling industry in influencing and perpetuating gambling behaviour in Ghana. The aim of this study was to contribute to understanding the extent of gambling research in Ghana by mapping out what the current literature indicates. Six databases were searched for peer-reviewed journal articles that focused on gambling in Ghana. The findings from this review demonstrate gambling research in Ghana is in a nascent state with a predominant focus on individual factors and limited consideration of public health. The implications for future research are highlighted, including the requirement for a nationally representative study to understand the prevalence of gambling participation and harm, and the role of the gambling industry. Public health action grounded in sound evidence is urgently required to address the individual and community level problems associated with this harmful commodity.
A highly diversified NLR cluster in melon contains homologs that confer powdery mildew and aphid resistance
Authors: Boissot Nathalie , Chovelon Veronique , Rittener-Ruff Vincent , Giovinazzo Nathalie , Mistral Pascale , Pitrat Michel , Charpentier Myriam , Troadec Christelle , Bendahmane Abdelhafid , Dogimont Catherine
Published: December 2023

Podosphaera xanthii is the main causal agent of powdery mildew (PM) on Cucurbitaceae. In Cucumis melo, the Pm-w resistance gene, which confers resistance to P. xanthii, is located on chromosome 5 in a cluster of nucleotide-binding leucine-rich repeat receptors (NLRs). We used positional cloning and transgenesis, to isolate the Pm-wWMR 29 gene encoding a coiled-coil NLR (CC-NLR). Pm-wWMR 29 conferred high level of resistance to race 1 of PM and intermediate level of resistance to race 3 of PM. Pm-wWMR 29 turned out to be a homolog of the Aphis gossypii resistance gene Vat-1PI 161375. We confirmed that Pm-wWMR 29 did not confer resistance to aphids, while Vat-1PI 161375 did not confer resistance to PM. We showed that both homologs were included in a highly diversified cluster of NLRs, the Vat cluster. Specific Vat-1PI 161375 and Pm-wWMR 29 markers were present in 10% to 13% of 678 accessions representative of wild and cultivated melon types worldwide. Phylogenic reconstruction of 34 protein homologs of Vat-1PI 161375 and Pm-wWMR 29 identified in 24 melon accessions revealed an ancestor with four R65aa—a specific motif in the LRR domain, evolved towards aphid and virus resistance, while an ancestor with five R65aa evolved towards PM resistance. The complexity of the cluster comprising the Vat/Pm-w genes and its diversity in melon suggest that Vat homologs may contribute to the recognition of a broad range of yet to be identified pests and pathogens.
A telomere-to-telomere reference genome of ficus (Ficus hispida) provides new insights into sex determination
Authors: Liao Zhenyang , Zhang Tianwen , Lei Wenlong , Wang Yibin , Yu Jiaxin , Wang Yinghao , Chai Kun , Wang Gang , Zhang Huahao , Zhang Xingtan
Published: December 2023

A high-quality reference genome is indispensable for resolving biologically essential traits. Ficus hispida is a dioecious plant. A complete Ficus reference genome will be crucial for understanding their sex evolution and important biological characteristics, such as aerial roots, mutualistic symbiosis with ficus-wasps, and fruiting from old stems. Here, we generated a telomere-to-telomere (T2T) genome for F. hispida using PacBio HiFi and Oxford Nanopore Ultra-long sequencing technologies. The genome contiguity and completeness has shown improvement compared with the previously released genome, with the annotation of six centromeres and 28 telomeres. We have refined our previously reported 2-Mb male-specific region into a 7.2-Mb genomic region containing 51 newly predicted genes and candidate sex-determination genes AG2 and AG3. Many of these genes showed extremely low expression, likely attributed to hypermethylation in the gene body and promoter regions. Gene regulatory networks (GRNs) revealed that AG2 and AG3 are related to the regulation of stamen development in male flowers, while the AG1 gene is responsible for regulating female flowers’ defense responses and secondary metabolite processes. Comparative analysis of GRNs showed that the NAC, WRKY, and MYB transcription factor families dominate the female GRN, whereas the MADS and MYB transcription factor families are prevalent in the male GRN.
A biosynthetic network for protoberberine production in Coptis chinensis
Authors: Wu Linrui , Zhao Binxin , Deng Zixin , Wang Bin , Yu Yi
Published: December 2023

Protoberberine alkaloids are a group of tetracyclic isoquinoline compounds known for their well-established antimicrobial and anti-inflammatory properties. The richness and diversity of protoberberine alkaloids accumulated in the Coptis genus necessitate a comprehensive examination of the biosynthetic machinery to understand their ecological significance. Here, from Coptis chinensis we identified CcCYP719A1, which could install a methylenedioxy bridge on either ring A or ring D of the protoberberine backbone, thus diverging metabolite flux towards the biosynthesis of various protoberberine components. We also obtained CcCYP719A2 and CcCYP719A3, which underwent positive selection after diverging from CcCYP719A1 and maintained specific catalytic activity on ring D. Further, we resolved the biosynthetic pathway of jatrorrhizine by identifying two demethylases, which could also modulate protoberberine composition by removing the C-3 methyl group and methylenedioxy bridge of ring D, allowing demethylated metabolites to be redirected into different routes. Moreover, we characterized 2-O-methyltransferase CcOMT1 and flavin-dependent oxidase CcTHBO, respectively responsible for the commonly observed 2-O-methylation and aromatic ring-C assembly in protoberberine alkaloids. Overall, this study reveals an interconnected metabolite network from which diverse protoberberine alkaloids originate. It provides valuable insights into the existence of undiscovered protoberberine components, and paves the way for the targeted production of desired protoberberine components for potential therapeutic development.
A Ca2+ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage
Authors: Piao Yinglan , Li Shizhen , Chen Yiduo , Zhao Sisi , Piao Zhongyun , Wang Haiping
Published: December 2023

Clubroot disease caused by Plasmodiophora brassicae (P. brassicae) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane, which can mediate the cytosolic Ca2+ increase in plant cells upon pathogen attack. However, the downstream Ca2+ sensor and decoder are still unknown. In this study, we identified the virulent and avirulent P. brassicae isolates (Pbs) of two near isogenic lines, CR 3–2 and CS 3–2, with CR 3–2 harboring clubroot resistant gene BraCRa. The transcriptomic analysis was then conducted with CR 3–2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca2+-sensor encoding gene, BraCBL1.2, that was highly induced in CR 3–2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+ sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P. brassicae. In addition, we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis. Collectively, our data identified the involvement of a Ca2+ sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to P. brassicae.
Comprehensive dissection of variation and accumulation of free amino acids in tea accessions
Authors: Huang Rong , Wang Zhihua , Wen Weiwei , Yao Mingzhe , Liu Haoran , Li Fang , Zhang Shuran , Ni Dejiang , Chen Liang
Published: December 2023

Free amino acids (FAAs) positively determine the tea quality, notably theanine (Thea), endowing umami taste of tea infusion, which is the profoundly prevalent research in albino tea genetic resources. Therefore, 339 tea accessions were collected to study FAAs level for deciphering its variation and accumulation mechanism. Interestingly, alanine (Ala) and Thea which had the highest diversity index (H′) value among three varieties of Camellia sinensis (L.) O. Kuntze were significantly higher than wild relatives (P < 0.05). The intraspecific arginine (Arg) and glutamine (Gln) contents in C. sinensis var. assamica were significantly lower than sinensis and pubilimba varieties. Moreover, the importance of interdependencies operating across FAAs and chlorophyll levels were highlighted via the cell ultrastructure, metabolomics, and transcriptome analysis. We then determined that the association between phytochrome interacting factor 1 (CsPIF1) identified by weighted gene co-expression network analysis (WGCNA) and Thea content. Intriguingly, transient knock-down CsPIF1 expression increased Thea content in tea plant, and the function verification of CsPIF1 in Arabidopsis also indicated that CsPIF1 acts as a negative regulator of Thea content by mainly effecting the genes expression related to Thea biosynthesis, transport, and hydrolysis, especially glutamate synthase (CsGOGAT), which was validated to be associated with Thea content with a nonsynonymous SNP by Kompetitive Allele-Specific PCR (KASP). We also investigated the interspecific and geographical distribution of this SNP. Taken together, these results help us to understand and clarify the variation and profile of major FAAs in tea germplasms and promote efficient utilization in tea genetic improvement and breeding.
Fertility education: recommendations for developing and implementing tools to improve fertility literacy
Authors: Martins Mariana V , Koert Emily , Sylvest Randi , Maeda Eri , Moura-Ramos Mariana , Hammarberg Karin , Harper Joyce
Published: December 2023

Many recent societal trends have led to the need for fertility education, including the age at which individuals become parents, the development of new reproductive technologies, and family diversity. Fertility awareness has emerged as a concept very recently and is increasingly gaining recognition. However, fertility education is often neglected as there is no consensus on the appropriate content, target populations, or on who should provide it. This article attempts to provide an overview of the use of interventions to improve fertility education. We emphasize the importance of delivering evidence-based information on fertility and reproductive health through various methods while providing guidelines for their standardization and systematization. Recommendations are provided to aid the development and implementation of fertility education tools, including: the establishment of a comprehensive understanding of the target populations; the incorporation of theories of behavioural change; the inclusion of the users’ perspectives and the use of participatory research; and the use of specific guidelines for increasing engagement. By following these recommendations, it is expected that fertility education resources can contribute to improving fertility literacy, empowering individuals and couples to make informed reproductive decisions, and ultimately reducing the incidence of infertility and need for fertility treatment.
Subinhibitory effects of 2,4-diacetylphloroglucinol on filamentous fungus Aspergillus fumigatus
Authors: Stepanov Artyom A , Vasilchenko Anastasia V , Vasilchenko Alexey S
Published: December 2023

Aims

Aspergillus fungi are common members of the soil microbiota. Some physiological and structural characteristics of Aspergillus species make them important participants in soil ecological processes. In this study, we aimed to evaluate the impact of 2,4-diacetylphloroglucinol (2,4-DAPG), a common metabolite of soil and rhizosphere bacteria, on the physiology of Aspergillus fumigatus.

Methods and results

Integrated analysis using microscopy, spectrophotometry, and liquid chromatography showed the following effects of 2,4-DAPG on Aspergillus physiology. It was found that A. fumigatus in the biofilm state is resistant to high concentrations of 2,4-DAPG. However, experimental exposure led to a depletion of the extracellular polymeric substance, changes in the structure of the cell wall of the mycelium (increase in the content of α- and β-glucans, chitin, and ergosterol), and conidia (decrease in the content of DHN-melanin). 2,4-DAPG significantly reduced the production of mycotoxins (gliotoxin and fumagillin) but increased the secretion of proteases and galactosaminogalactan.

Conclusions

Overall, the data obtained suggest that 2,4-DAPG-producing Pseudomonas bacteria are unlikely to directly eliminate A. fumigatus fungi, as they exhibit a high level of resistance when in the biofilm state. However, at low concentrations, 2,4-DAPG significantly alters the physiology of aspergilli, potentially reducing the adaptive and competitive capabilities of these fungi.
Antibiofilm potential of nanonized eugenol againstPseudomonas aeruginosa
Authors: Ghosh Sourav , Sett Upasana , Pal Anabadya , Nandy Sanchita , Nandi Susmita , Chakrabarty Soumajit , Das Abhijit , Bandopadhyay Pathikrit , Basu Tarakdas
Published: December 2023

Aims

The purpose of this study was to synthesize a nanoform of eugenol (an important phytochemical with various pharmacological potentials) and to investigate its antibiofilm efficacy on Pseudomonas aeruginosa biofilm.

Methods and results

Colloidal suspension of eugenol-nanoparticles (ENPs) was synthesized by the simple ultrasonic cavitation method through the emulsification of hydrophobic eugenol into hydrophilic gelatin. Thus, the nanonization process made water-insoluble eugenol into water-soluble nano-eugenol, making the nanoform bioavailable. The size of the ENPs was 20–30 nm, entrapment efficiency of eugenol within gelatin was 80%, and release of eugenol from the gelatin cap was slow and sustained over 5 days. Concerning the clinically relevant pathogen P. aeruginosa, ENPs had higher antibiofilm (for both formation and eradication) activities than free eugenol. Minimal biofilm inhibitory concentration and minimal biofilm eradication concentration of ENP on P. aeruginosa biofilm were 2.0 and 4.0 mM, respectively. In addition, the measurement of P. aeruginosa biofilm biomass, biofilm thickness, amount of biofilm extra-polymeric substance, cell surface hydrophobicity, cell swarming and twitching efficiencies, cellular morphology, and biofilm formation in catheter demonstrated that the antibiofilm efficacy of nano-eugenol was 30%–40% higher than that of bulk eugenol.

Conclusion

These results signify that future pharmacological and clinical studies are very much required to investigate whether ENPs can act as an effective drug against P. aeruginosa biofilm-mediated diseases. Thus, the problem of intrinsic antibiotic tolerance of biofilm-forming cells may be minimized by ENPs. Moreover, ENP may be used as a potential catheter-coating agent to inhibit pseudomonal colonization on catheter surfaces and, therefore, to reduce catheter-associated infections and complications.