bluuebunny/crossref_metadata_2025 huggingface dataset:
Perspectives and Experiences of Public Safety Personnel Engaged in a Peer-Led Workplace Reintegration Program Post Critical Incident or Operational Stress Injury: A Qualitative Thematic Analysis
Authors: Jones Chelsea , Spencer Shaylee , O’Greysik Elly , Smith-MacDonald Lorraine , Bright Katherine S. , Beck Amy J. , Carleton R. Nicholas , Burback Lisa , Greenshaw Andrew , Zhang Yanbo , Sevigny Phillip R. , Hayward Jake , Cao Bo , Brémault-Phillips Suzette
Published: July 2024

Introduction: Public safety personnel (PSP) experience operational stress injuries (OSIs), which can put them at increased risk of experiencing mental health and functional challenges. Such challenges can result in PSP needing to take time away from the workplace. An unsuccessful workplace reintegration process may contribute to further personal challenges for PSP and their families as well as staffing shortages that adversely affect PSP organizations. The Canadian Workplace Reintegration Program (RP) has seen a global scale and spread in recent years. However, there remains a lack of evidence-based literature on this topic and the RP specifically. The current qualitative study was designed to explore the perspectives of PSP who had engaged in a Workplace RP due to experiencing a potentially psychologically injurious event or OSI. Methods: A qualitative thematic analysis analyzed interview data from 26 PSP who completed the RP. The researchers identified five themes: (1) the impact of stigma on service engagement; (2) the importance of short-term critical incident (STCI) program; (3) strengths of RP; (4) barriers and areas of improvement for the RP; and (5) support outside the RP. Discussion: Preliminary results were favorable, but further research is needed to address the effectiveness, efficacy, and utility of the RP. Conclusion: By addressing workplace reintegration through innovation and research, future initiatives and RP iterations can provide the best possible service and support to PSP and their communities.
Host Cells Upregulate Phosphate Transporter PIT1 to Inhibit Ehrlichia chaffeensis Intracellular Growth
Authors: Li Meifang , Yang Nan , Li Xiaoxiao , Duan Nan , Qin Shanhua , Wang Mengyao , Zhou Yuhong , Jin Yongxin , Wu Weihui , Jin Shouguang , Cheng Zhihui
Published: July 2024

Ehrlichia chaffeensis infects and proliferates inside monocytes or macrophages and causes human monocytic ehrlichiosis (HME), an emerging life-threatening tick-borne zoonosis. After internalization, E. chaffeensis resides in specialized membrane-bound inclusions, E. chaffeensis-containing vesicles (ECVs), to evade from host cell innate immune responses and obtain nutrients. However, mechanisms exploited by host cells to inhibit E. chaffeensis growth in ECVs are still largely unknown. Here we demonstrate that host cells recognize E. chaffeensis Ech_1067, a penicillin-binding protein, and then upregulate the expression of PIT1, which is a phosphate transporter and transports phosphate from ECVs to the cytosol to inhibit bacterial growth. We found that host cells upregulate the PIT1 expression upon E. chaffeensis infection using transcriptome sequencing, qRT-PCR and Western blotting, and PIT1 is localized on the ECV membrane in infected THP-1 cells using confocal microscopy. Silence of PIT1 using shRNA enhances E. chaffeensis intracellular growth. Finally, we found that E. chaffeensis Ech_1067 induces the upregulation of PIT1 expression through the MyD88-NF-κB pathway using recombinant protein for stimulation and siRNA for silence. Our findings deepen the understanding of the innate immune responses of host cells to inhibit bacterial intracellular growth and facilitate the development of new therapeutics for HME.
In Pursuit of Novel Markers: Unraveling the Potential of miR-106, CEA and CA 19-9 in Gastric Adenocarcinoma Diagnosis and Staging
Authors: Boicean Adrian , Boeras Ioana , Birsan Sabrina , Ichim Cristian , Todor Samuel Bogdan , Onisor Danusia Maria , Brusnic Olga , Bacila Ciprian , Dura Horatiu , Roman-Filip Corina , Ognean Maria Livia , Tanasescu Ciprian , Hasegan Adrian , Bratu Dan , Porr Corina , Roman-Filip Iulian , Neamtu Bogdan , Fleaca Sorin Radu
Published: July 2024

Gastric cancer stands as the fourth leading cause of cancer-related deaths globally, primarily comprising adenocarcinomas, categorized by anatomic location and histologic type. Often diagnosed at advanced stages, gastric cancer prognosis remains poor. To address the critical need for accurate tumoral markers for gastric cancer diagnosis, we conducted a study to assess classical markers like CEA and CA-19-9 alongside the novel marker miR-106. Our investigation revealed distinct dynamics of these markers compared to non-cancerous groups, although no disparities were observed across different disease stages. Univariable and multivariable logistic regression analyses demonstrated that elevated levels of miR-106, CEA and CA 19-9 were predictive of a positive histopathological exam, with the respective odds ratios of 12.032 (95% CI: 1.948–74.305), 30 (95% CI: 3.141–286.576), and 55.866 (95% CI: 4.512–691.687). Subsequently, we utilized predicted probabilities from regression models to construct receiver operating characteristic (ROC) curves, identifying CA 19-9 as the optimal predictor for gastric adenocarcinoma diagnosis when considering age and gender, with an area under the curve (AUC) of 0.936 (p < 0.001). Hence, classical markers exhibit superior performance compared to the novel marker miR-106 in predicting gastric adenocarcinoma.
Profound Properties of Protein-Rich, Platelet-Rich Plasma Matrices as Novel, Multi-Purpose Biological Platforms in Tissue Repair, Regeneration, and Wound Healing
Authors: Everts Peter A. , Lana José Fábio , Alexander Robert W. , Dallo Ignacio , Kon Elizaveta , Ambach Mary A. , van Zundert André , Podesta Luga
Published: July 2024

Autologous platelet-rich plasma (PRP) preparations are prepared at the point of care. Centrifugation cellular density separation sequesters a fresh unit of blood into three main fractions: a platelet-poor plasma (PPP) fraction, a stratum rich in platelets (platelet concentrate), and variable leukocyte bioformulation and erythrocyte fractions. The employment of autologous platelet concentrates facilitates the biological potential to accelerate and support numerous cellular activities that can lead to tissue repair, tissue regeneration, wound healing, and, ultimately, functional and structural repair. Normally, after PRP preparation, the PPP fraction is discarded. One of the less well-known but equally important features of PPP is that particular growth factors (GFs) are not abundantly present in PRP, as they reside outside of the platelet alpha granules. Precisely, insulin-like growth factor-1 (IGF-1) and hepatocyte growth factor (HGF) are mainly present in the PPP fraction. In addition to their roles as angiogenesis activators, these plasma-based GFs are also known to inhibit inflammation and fibrosis, and they promote keratinocyte migration and support tissue repair and wound healing. Additionally, PPP is known for the presence of exosomes and other macrovesicles, exerting cell–cell communication and cell signaling. Newly developed ultrafiltration technologies incorporate PPP processing methods by eliminating, in a fast and efficient manner, plasma water, cytokines, molecules, and plasma proteins with a molecular mass (weight) less than the pore size of the fibers. Consequently, a viable and viscous protein concentrate of functional total proteins, like fibrinogen, albumin, and alpha-2-macroglobulin is created. Consolidating a small volume of high platelet concentrate with a small volume of highly concentrated protein-rich PPP creates a protein-rich, platelet-rich plasma (PR-PRP) biological preparation. After the activation of proteins, mainly fibrinogen, the PR-PRP matrix retains and facilitates interactions between invading resident cells, like macrophages, fibroblast, and mesenchymal stem cells (MSCs), as well as the embedded concentrated PRP cells and molecules. The administered PR-PRP biologic will ultimately undergo fibrinolysis, leading to a sustained release of concentrated cells and molecules that have been retained in the PR-PRP matrix until the matrix is dissolved. We will discuss the unique biological and tissue reparative and regenerative properties of the PR-PRP matrix.
Metabolic Shift in Porcine Spermatozoa during Sperm Capacitation-Induced Zinc Flux
Authors: Weide Tyler , Mills Kayla , Shofner Ian , Breitzman Matthew W. , Kerns Karl
Published: July 2024

Mammalian spermatozoa rely on glycolysis and mitochondrial oxidative phosphorylation for energy leading up to fertilization. Sperm capacitation involves a series of well-regulated biochemical steps that are necessary to give spermatozoa the ability to fertilize the oocyte. Additionally, zinc ion (Zn2+) fluxes have recently been shown to occur during mammalian sperm capacitation. Semen from seven commercial boars was collected and analyzed using image-based flow cytometry before, after, and with the inclusion of 2 mM Zn2+ containing in vitro capacitation (IVC) media. Metabolites were extracted and analyzed via Gas Chromatography-Mass Spectrometry (GC-MS), identifying 175 metabolites, with 79 differentially abundant across treatments (p < 0.05). Non-capacitated samples showed high levels of respiration-associated metabolites including glucose, fructose, citric acid, and pyruvic acid. After 4 h IVC, these metabolites significantly decreased, while phosphate, lactic acid, and glucitol increased (p < 0.05). With zinc inclusion, we observed an increase in metabolites such as lactic acid, glucitol, glucose, fructose, myo-inositol, citric acid, and succinic acid, while saturated fatty acids including palmitic, dodecanoic, and myristic acid decreased compared to 4 h IVC, indicating regulatory shifts in metabolic pathways and fatty acid composition during capacitation. These findings underscore the importance of metabolic changes in improving artificial insemination and fertility treatments in livestock and humans.
Bicalutamide Enhances Conventional Chemotherapy in In Vitro and In Vivo Assays Using Human and Canine Inflammatory Mammary Cancer Cell Lines
Authors: Crespo Belen , Illera Juan Carlos , Silvan Gema , Lopez-Plaza Paula , Herrera de la Muela María , de la Puente Yague Miriam , Diaz del Arco Cristina , de Andrés Paloma Jimena , Illera Maria Jose , Caceres Sara
Published: July 2024

Human inflammatory breast cancer (IBC) and canine inflammatory mammary cancer (IMC) are highly aggressive neoplastic diseases that share numerous characteristics. In IBC and IMC, chemotherapy produces a limited pathological response and anti-androgen therapies have been of interest for breast cancer treatment. Therefore, the aim was to evaluate the effect of a therapy based on bicalutamide, a non-steroidal anti-androgen, with doxorubicin and docetaxel chemotherapy on cell proliferation, migration, tumor growth, and steroid-hormone secretion. An IMC-TN cell line, IPC-366, and an IBC-TN cell line, SUM149, were used. In vitro assays revealed that SUM149 exhibited greater sensitivity, reducing cell viability and migration with all tested drugs. In contrast, IPC-366 exhibited only significant in vitro reductions with docetaxel as a single agent or in different combinations. Decreased estrogen levels reduced in vitro tumor growth in both IMC and IBC. Curiously, doxorubicin resulted in low efficacy, especially in IMC. In addition, all drugs reduced the tumor volume in IBC and IMC by increasing intratumoral testosterone (T) levels, which have been related with reduced tumor progression. In conclusion, the addition of bicalutamide to doxorubicin and docetaxel combinations may represent a potential treatment for IMC and IBC.
Recombinant Expression and Characterization of a Novel Thermo-Alkaline Lipase with Increased Solvent Stability from the Antarctic Thermophilic Bacterium Geobacillus sp. ID17
Authors: Salas-Bruggink Diego , Guzmán Hardy , Espina Giannina , Blamey Jenny M.
Published: July 2024

Lipases are enzymes that hydrolyze long-chain carboxylic esters, and in the presence of organic solvents, they catalyze organic synthesis reactions. However, the use of solvents in these processes often results in enzyme denaturation, leading to a reduction in enzymatic activity. Consequently, there is significant interest in identifying new lipases that are resistant to denaturing conditions, with extremozymes emerging as promising candidates for this purpose. Lip7, a lipase from Geobacillus sp. ID17, a thermophilic microorganism isolated from Deception Island, Antarctica, was recombinantly expressed in E. coli C41 (DE3) in functional soluble form. Its purification was achieved with 96% purity and 23% yield. Enzymatic characterization revealed Lip7 to be a thermo-alkaline enzyme, reaching a maximum rate of 3350 U mg−1 at 50 °C and pH 11.0, using p-nitrophenyl laurate substrate. Notably, its kinetics displayed a sigmoidal behavior, with a higher kinetic efficiency (kcat/Km) for substrates of 12-carbon atom chain. In terms of thermal stability, Lip7 demonstrates stability up to 60 °C at pH 8.0 and up to 50 °C at pH 11.0. Remarkably, it showed high stability in the presence of organic solvents, and under certain conditions even exhibited enzymatic activation, reaching up to 2.5-fold and 1.35-fold after incubation in 50% v/v ethanol and 70% v/v isopropanol, respectively. Lip7 represents one of the first lipases from the bacterial subfamily I.5 and genus Geobacillus with activity and stability at pH 11.0. Its compatibility with organic solvents makes it a compelling candidate for future research in biocatalysis and various biotechnological applications.
Machine Learning to Estimate Workload and Balance Resources with Live Migration and VM Placement
Authors: Hidayat Taufik , Ramli Kalamullah , Thereza Nadia , Daulay Amarudin , Rushendra Rushendra , Mahardiko Rahutomo
Published: July 2024

Currently, utilizing virtualization technology in data centers often imposes an increasing burden on the host machine (HM), leading to a decline in VM performance. To address this issue, live virtual migration (LVM) is employed to alleviate the load on the VM. This study introduces a hybrid machine learning model designed to estimate the direct migration of pre-copied migration virtual machines within the data center. The proposed model integrates Markov Decision Process (MDP), genetic algorithm (GA), and random forest (RF) algorithms to forecast the prioritized movement of virtual machines and identify the optimal host machine target. The hybrid models achieve a 99% accuracy rate with quicker training times compared to the previous studies that utilized K-nearest neighbor, decision tree classification, support vector machines, logistic regression, and neural networks. The authors recommend further exploration of a deep learning approach (DL) to address other data center performance issues. This paper outlines promising strategies for enhancing virtual machine migration in data centers. The hybrid models demonstrate high accuracy and faster training times than previous research, indicating the potential for optimizing virtual machine placement and minimizing downtime. The authors emphasize the significance of considering data center performance and propose further investigation. Moreover, it would be beneficial to delve into the practical implementation and dissemination of the proposed model in real-world data centers.
Future Range Expansions of Invasive Wasps Suggest Their Increasing Impacts on Global Apiculture
Authors: Zhang Xueyou , Nie Peixiao , Hu Xiaokang , Feng Jianmeng
Published: July 2024

Until now, no study has examined the future range dynamics of major invasive wasp species to assess their future impacts on global apiculture. Here, we developed 12 species distribution models to calibrate the future range dynamics of 12 major invasive Vespidae wasp species under a unified framework. An increase in their habitat suitability was identified in more than 75% of global land. Substantial range expansions were detected for all 12 species, and they were primarily induced by future climate changes. Notably, Polistes dominula and Vespa crabro had the largest potential ranges under all scenarios, suggesting their greater impact on global apiculture. Polistes chinensis and Vespa velutina nigrithorax had the highest range expansion ratios, so they warrant more urgent attention than the other species. Polistes versicolor and P. chinensis are expected to exhibit the largest centroid shifts, suggesting that substantial shifts in prioritizing regions against their invasions should be made. Europe and the eastern part of the USA were future invasion hotspots for all major invasive wasp species, suggesting that apiculture might face more pronounced threats in these regions than in others. In conclusion, given their substantial range shifts, invasive wasps will likely have increasingly negative impacts on global apiculture in the future.
New Therapeutics for Heart Failure Worsening: Focus on Vericiguat
Authors: Russo Patrizia , Vitiello Laura , Milani Francesca , Volterrani Maurizio , Rosano Giuseppe M. C. , Tomino Carlo , Bonassi Stefano
Published: July 2024

Heart failure (HF) is a syndrome characterized by signs and symptoms resulting from structural or functional cardiac abnormalities, confirmed by elevated natriuretic peptides or evidence of congestion. HF patients are classified according to left ventricular ejection fraction (LVEF). Worsening HF (WHF) is associated with increased short- and long-term mortality, re-hospitalization, and healthcare costs. The standard treatment of HF includes angiotensin-converting enzyme inhibitors, angiotensin receptor–neprilysin inhibitors, mineralocorticoid-receptor antagonists, beta-blockers, and sodium-glucose-co-transporter 2 inhibitors. To manage systolic HF by reducing mortality and hospitalizations in patients experiencing WHF, treatment with vericiguat, a direct stimulator of soluble guanylate cyclase (sGC), is indicated. This drug acts by stimulating sGC enzymes, part of the nitric oxide (NO)–sGC–cyclic guanosine monophosphate (cGMP) signaling pathway, regulating the cardiovascular system by catalyzing cGMP synthesis in response to NO. cGMP acts as a second messenger, triggering various cellular effects. Deficiencies in cGMP production, often due to low NO availability, are implicated in cardiovascular diseases. Vericiguat stimulates sGC directly, bypassing the need for a functional NO-sGC-cGMP axis, thus preventing myocardial and vascular dysfunction associated with decreased sGC activity in heart failure. Approved by the FDA in 2021, vericiguat administration should be considered, in addition to the four pillars of reduced EF (HFrEF) therapy, in symptomatic patients with LVEF < 45% following a worsening event. Cardiac rehabilitation represents an ideal setting where there is more time to implement therapy with vericiguat and incorporate a greater number of medications for the management of these patients. This review covers vericiguat’s metabolism, molecular mechanisms, and drug–drug interactions.
Management of Malignant Bowel Obstruction in Patients with Gynaecological Cancer: A Systematic Review
Authors: Tóth Richárd , Tóth Zsófia , Lőczi Lotti , Török Marianna , Ács Nándor , Várbíró Szabolcs , Keszthelyi Márton , Lintner Balázs
Published: July 2024

Objectives: This systematic review aimed to evaluate current surgical and non-surgical management strategies for malignant bowel obstruction (MBO) in patients with gynaecological cancer. Methods: Comprehensive literature searches were conducted across MEDLINE, Embase, CENTRAL, and Scopus, without restrictions on language or publication date. Following the removal of duplicates, 4866 articles were screened, with 34 meeting the inclusion criteria. Results: Surgical intervention remains the definitive treatment for MBO, offering longer symptom-free periods and improved survival, particularly when conservative methods fail. However, the selection of surgical candidates is crucial due to the high risk of morbidity and the potential for significant complications. Non-surgical treatments, such as the use of Gastrografin, Octreotide, and Dexamethasone, along with invasive procedures like nasogastric tubing, percutaneous gastrostomy, and stent placement, offer varying degrees of symptom relief and are often considered when surgery is not feasible. Conclusions: In this article we provide a potential therapeutic algorithm for the management of patients with MBO. This review underscores the urgent need for high-quality research to develop clear, evidence-based guidelines for MBO management in patients with gynaecologic cancer. Establishing standardised protocols will improve patient outcomes by aiding clinicians in making informed, individualised treatment decisions.
Effectiveness of Clear Aligners on Sequential Maxillary Molar Distalization: Discrepancy between Treatment Goal and Outcome
Authors: Mamani Jatuphol , Sessirisombat Chidchanok , Hotokezaka Hitoshi , Yoshida Noriaki , Sirisoontorn Irin
Published: July 2024

Objectives: The purpose of this preliminary study was to determine the differences between planned and actual maxillary molar movements after the completion of treatment with an initial set of clear aligners including sequential maxillary molar distalization. Methods: The data records of 14 non-growing patients who completed orthodontic treatment with sequential maxillary molar distalization using clear aligners were retrospectively evaluated (n= 14, 4 males and 10 females, 33.61 ± 8.57 years). Data on planned tooth movements were obtained from ClinCheck software (ClinCheck Pro version 5.3). The amounts of actual tooth movements were obtained by performing superimposition of lateral cephalograms taken before and after treatment. The amounts of distal translation and tipping between planned and actual maxillary molar movements were compared with the paired Student’s t-test. Results: The statistically significant differences between planned and actual translation movements of maxillary first and second molars were shown after completing treatment with the first series of aligners (p < 0.05). The average actual amount of molar distalization on maxillary first molars was less than the planned amount by 1.32 ± 0.42 mm. Similarly, the average actual amount of molar distalization on maxillary second molars was less than the planned amount by 1.57 ± 0.45 mm. The accuracy for molar distalization, namely, the percentage of actual distal translation to planned movement, was 40.11% for maxillary first molars and 35.39% for maxillary second molars. However, the difference between the planned and actual angulation movements was not significant (p > 0.05). Conslusions: In conclusion, the amounts of actual distal translation of maxillary molars through the utilization of clear aligners were significantly lower than planned. However, there were no statistically significant differences between the degrees of actual and planned molar angulation movement.
Differences in Eotaxin Serum Levels between Polytraumatized Patients with and without Concomitant Traumatic Brain Injury—A Matched Pair Analysis
Authors: Negrin Lukas L. , Ristl Robin , Wollner Gregor , Hajdu Stefan
Published: July 2024

Background/Objectives: Early detection of traumatic brain injury (TBI) is crucial for minimizing secondary neurological damage. Our study aimed to assess the potential of IL-4, IL-6, IL-7, IL-8, IL-10, TNF, and eotaxin serum levels—as a single clinical tool or combined into a panel—for diagnosing TBI in multiple injured patients. Methods: Out of 110 prospectively enrolled polytrauma victims (median age, 39 years; median ISS, 33; 70.9% male) admitted to our level I trauma center over four years, we matched 41 individuals with concomitant TBI (TBI cohort) to 41 individuals without TBI (non-TBI cohort) based on age, gender, Injury Severity Score (ISS), and mortality. Patients’ protein levels were measured upon admission (day 0) and on days 1, 3, 5, 7, and 10 during routine blood withdrawal using one separation gel tube each time. Results: The median serum levels of IL-4, IL-6, IL-7, IL-8, IL-10, and TNF exhibited non-similar time courses in the two cohorts and showed no significant differences on days 0, 1, 3, 5, and 7. However, the median eotaxin levels had similar trend lines in both cohorts, with consistently higher levels in the TBI cohort, reaching significance on days 0, 3, and 5. In both cohorts, the median eotaxin level significantly decreased from day 0 to day 1, then significantly increased until day 10. We also found a significant positive association between day 0 eotaxin serum levels and the presence of TBI, indicating that for every 20 pg/mL increase in eotaxin level, the odds of a prevalent TBI rose by 10.5%. ROC analysis provided a cutoff value of 154 pg/mL for the diagnostic test (sensitivity, 0.707; specificity, 0.683; AUC = 0.718). Conclusions: Our findings identified the brain as a significant source, solely of eotaxin release in humans who have suffered a TBI. Nevertheless, the eotaxin serum level assessed upon admission has limited diagnostic value. IL-4, IL-6, IL-7, IL-8, IL-10, and TNF do not indicate TBI in polytraumatized patients.
Beyond Trauma-Induced Coagulopathy: Detection of Auto-Heparinization as a Marker of Endotheliopathy Using Rotational Thromboelastometry
Authors: Băetu Alexandru Emil , Mirea Liliana , Cobilinschi Cristian , Grințescu Ioana Cristina , Grințescu Ioana Marina
Published: July 2024

Background/Objectives: The complexity of trauma-induced coagulopathy (TIC) is a result of the unique interactions between the patient, trauma, and resuscitation-related causes. The main objective of trauma resuscitation is to create the optimal milieu for both the development of immediate reparatory mechanisms and the prevention of further secondary injuries. Endotheliopathy represents one of the hallmarks of trauma-induced coagulopathy, and comprises endothelial dysfunction, abnormal coagulation, and inflammation, all of which arise after severe trauma and hemorrhagic shock. Methods: We retrospectively and descriptively evaluated 217 patients admitted to the Bucharest Clinical Emergency Hospital who met the Berlin criteria for the diagnosis of multiple trauma. Patients with high suspicion of auto-heparinization were identified according to the dynamic clinical and para-clinical evolution and subsequently tested using rotational thromboelastometry (ROTEM). The ratio between the clot formation time (CT) was used, obtained on the two channels of interest (INTEM/HEPTEM). Results: Among the 217 patients with a mean age of 43.43 ± 15.45 years and a mean injury severity score (ISS) of 36.98 ± 1.875, 42 patients had a reasonable clinical and para-clinical suspicion of auto-heparinization, which was later confirmed by the INTEM/HEPTEM clotting time ratio in 28 cases (12.9% from the entire study population). A multiple linear regression analysis highlighted that serum lactate (estimated 0.02, p = 0.0098) and noradrenaline requirement (estimated 0.03, p = 0.0053) influenced the CT (INTEM/HEPTEM) ratio. Conclusions: There is a subset of multiple trauma patients in which the CT (INTEM/HEPTEM) ratio was influenced only by serum lactate levels and patients’ need for vasopressor use, reinforcing the relationship between shock, hypoperfusion, and clotting derangements. This emphasizes the unique response that each patient has to trauma.
Clinical Insights and Future Directions in Hypothermia for Severe Traumatic Brain Injury: A Narrative Review
Authors: Kobata Hitoshi
Published: July 2024

Fever control is essential in patients with severe traumatic brain injury (TBI). The efficacy of therapeutic hypothermia (TH) in severe TBI has been investigated over the last few decades; however, in contrast to experimental studies showing benefits, no evidence of efficacy has been demonstrated in clinical practice. In this review, the mechanisms and history of hypothermia were briefly outlined, while the results of major randomized controlled trials (RCTs) and meta-analyses investigating TH for adult TBI were introduced and discussed. The retrieved meta-analyses showed conflicting results, with a limited number of studies indicating the benefits of TH. Some studies have shown the benefits of long-term TH compared with short-term TH. Although TH is effective at lowering elevated intracranial pressure (ICP), reduced ICP does not lead to favorable outcomes. Low-quality RCTs overestimated the benefits of TH, while high-quality RCTs showed no difference or worse outcomes with TH. RCTs assessing standardized TH quality demonstrated the benefits of TH. As TBI has heterogeneous and complicated pathologies, applying a uniform treatment may not be ideal. A meta-analysis of young patients who underwent early cooling and hematoma removal showed better TH results. TH should not be abandoned, and its optimal usage should be advocated on an individual basis.
Revisiting Latex-Fruit Syndrome after 30 Years of Research: A Comprehensive Literature Review and Description of Two Cases
Authors: Gromek Weronika , Kołdej Natalia , Świtała Szymon , Majsiak Emilia , Kurowski Marcin
Published: July 2024

Thirty years have passed since the concept of latex-fruit syndrome (LFS) was first introduced. Since then, this phenomenon, characterized by cross-reactivity between natural latex rubber allergens and certain fruit allergens, has been extensively studied. This literature review sought to determine the prevalence of LFS in latex-allergic patients, identify the most common cross-reactions with fruit allergens in individuals with LFS, and understand the clinical manifestations of this syndrome. An extensive literature search was carried out using PubMed and Scopus databases, while applying the Preferred Reporting Items for Systematic Reviews and Meta-Analysis methodology. The analysis of original studies revealed a wide variation in LFS prevalence (4–88%) influenced by diverse diagnostic tools, different geographical regions, and the size of study populations. Our findings indicate that the most prevalent allergenic fruits in patients with LFS are banana, avocado, kiwifruit, and papaya. After evaluating the symptoms of the fruit hypersensitivity of patients with LFS, the clinical manifestation of hypersensitivity constituted 73% of systemic allergy symptoms and only 27% of reported symptoms described the localized allergy. Furthermore, the clinical picture of latex-fruit syndrome is illustrated through two cases, one typical and one with an unusual presentation. Their clinical features were assessed and contrasted utilizing different anaphylaxis severity grading criteria. To properly manage LFS, it is essential to establish standardized diagnostic criteria and severity grading systems, as these are crucial for accurate diagnosis and effective treatment.
Summary of the Therapeutic Options for Patients with Dry and Neovascular AMD
Authors: Śpiewak Dorota , Drzyzga Łukasz , Dorecka Mariola , Wyględowska-Promieńska Dorota
Published: July 2024

Age-related macular degeneration (AMD) is the leading cause of irreversible blindness worldwide and a severe medical and social problem. The steadily increasing number of patients is related to the aging of the population. So far, many factors affecting the development of AMD have been identified, which can be divided into non-modifiable, including genetic factors, age, and sex, and modifiable or environmental factors, such as smoking, poor diet, and hypertension. Early stages of age-related macular degeneration are characterized by fundus drusen and abnormalities in the retinal pigment epithelium. In late stages, geographic atrophy and choroidal neovascularization (CNV) are observed. The treatment of AMD, especially its advanced forms, is very challenging. Intensive research has made it possible to treat advanced stages of the dry form of AMD with pegcetacoplan and avacincaptad pegol, new drugs approved for use in the US. Pegcetacoplan targets the C3 and avacincaptad pegol targets the C5, the pivotal proteins of the complement cascade. The drugs are administered by intravitreal injection. The gold standard for neovascular AMD (nAMD) consists of intravitreal injections of anti-vascular endothelial growth factor (anti-VEGF) drugs such as bevacizumab, ranibizumab, aflibercept, brolucizumab, and faricimab. Treatment can be administered according to the fixed, pro-re-nata, and treat-and-extend regimens. The latter seems to have the best effect on improving visual acuity (VA) and the maximum therapeutic benefit. The search continues for the best ways to deliver intravitreal drugs. Current methods include sustained-release implants and hydrogel platforms for drug release, while the most promising future pathways for treating dry and nAMD are stem cell and gene therapy.
Antiplatelet Therapy for Elderly Patients with Acute Coronary Syndrome Undergoing Percutaneous Coronary Intervention
Authors: Fioretti Vincenzo , Sperandeo Luca , Gerardi Donato , Di Fazio Aldo , Stabile Eugenio
Published: July 2024

The elderly represent an increasing proportion of patients presenting with acute coronary syndrome (ACS). Various data have shown that the benefits of percutaneous coronary revascularization are maintained in elderly patients presenting with ACS. Conversely, the management of antiplatelet therapy remains challenging and controversial, because older patients are usually at a high risk of both ischemia and bleeding. Moreover, the recommended ischemic and bleeding risk scores in patients with ACS were developed from studies with a low representation of older patients. New antiplatelet strategies have been developed, but their evidence in elderly patients is limited because they are usually underrepresented in randomized clinical trials due to their clinical complexity. The aim of this review is to summarize the different factors associated with increased ischemic and/or bleeding risk and the scientific evidence about the different antiplatelet strategies in elderly patients presenting with ACS and undergoing percutaneous coronary revascularization.
Use of the CardioMEMS Device in Children and Patients with Congenital Heart Disease: A Literature Review
Authors: Piccinelli Enrico , Grutter Giorgia , Pilati Mara , Rebonato Micol , Scalera Silvia Teresa , Adorisio Rachele , Amodeo Antonio , Ingrasciotta Gessica , Mencarelli Erica , Galletti Lorenzo , Butera Gianfranco
Published: July 2024

The CardioMEMS HF System (Abbott, Abbott Park, IL) is the first FDA- and CE-Mark-approved device for monitoring patients with heart failure, significantly reducing hospitalizations and improving the quality of life for NYHA class III non-congenital adult patients. This device, implanted percutaneously, allows the direct monitoring of pulmonary arterial pressure with the wireless transfer of pressure data to the clinician, who can adjust the therapy remotely. Limited experience exists regarding its use in patients with congenital heart disease (CHD). CardioMEMS device implantation is feasible and safe in selected adults and children with CHD. The potential of the device to reduce heart failure hospitalizations in this population is enormous, but further multi-center studies are needed to demonstrate its efficacy.
Successful Treatment of Cutaneous Squamous Cell Cancer with Cemiplimab—A Report of Two Cases Demonstrating the Management of Pseudoprogression and Adverse Events
Authors: Żukowska Paulina , Ciepiela Katarzyna , Kudrymska Aleksandra , Kiełbowski Kajetan , Becht Rafał
Published: July 2024

Background: Cutaneous squamous cell carcinoma is a common malignancy, which frequently develops in the areas exposed to the sun. Patients with locally advanced disease in the head and neck region are frequently disqualified from surgical resection and require systemic treatment. Methods: In this report, we present the clinicopathological features and treatment of two patients who received cemiplimab, a monoclonal antibody targeting programmed cell death receptor 1 (PD-1). Results: An 80-year-old female and 82-year-old male patient were admitted to the hospital for the treatment of large tumors diagnosed as squamous cell carcinomas. In both patients, surgical treatment was not recommended due to the large dimensions of the tumors. These patients qualified for systemic treatment with cemiplimab. In the first patient, immunotherapy was interrupted due to adverse events. Nevertheless, a continuous regression of the tumor was observed despite treatment cessation. The second patient experienced a pseudoprogression, which is an increase in the tumor size caused by infiltration of immune cells. The treatment significantly reduced tumor size in both patients, which highly improved their quality of life. Conclusions: Cemiplimab offers clinical benefits in patients with cutaneous squamous cell carcinoma who are ineligible for surgical treatment. Systemic treatment can significantly improve the quality of life and reduce tumor diameters.
Exploratory Study into the Classification Agreement between Self-Reported Age of Menarche and Calculated Maturity Offset in Adolescent Girls: A Two-Year Follow-Up Study
Authors: Gerber Barry , Pienaar Anita E.
Published: July 2024

Menarche is a significant pubertal event influencing girls’ participation in physical activity. As menarche is a sensitive matter, a non-invasive substitute is needed to help classify girls’ maturity status and provide physical literacy to them in this regard. The objective of this exploratory study was to investigate the classification agreement between self-reported age of menarche and calculated maturity offset in adolescent girls from South Africa by making use of various statistical methods. Fifty-eight girls, n = 13 pre- and n = 45 post-menarche (Status Quo method) aged 13.51 ± 3.51 years at baseline, were analyzed (2010–2012). Independent t-testing, cross-tabulation, Roc Curve statistics and logistic regression were used to analyze the classification agreement between markers. All four statistical methods revealed the potential to categorize different maturity groups through the maturity offset equation, although the accuracy declined with increased age. A realized power of 0.92 was found for the group in the first year of the study, with a gradual and significant decline over time. Cross-tabs showed a significant moderate predictive effectiveness (Chi-square = 0.042) during T1, closer to PHV (13.51 years) although also declining significantly with increased age (T2, 14.51 years) beyond PHV (Chi-square = 0.459). Although positive results were found, caution must be used when using maturity offset equations in different homogenic populations due to their unique growth characteristics.
Size-Dependent Microplastic Fragmentation Model
Authors: Pérez-Muñuzuri Vicente
Published: July 2024

Plastic fragmentation alters the size distribution of plastic waste in aquatic habitats, which is accelerated by mechanical stress and weathering degradation processes. Microplastic pieces constitute the vast bulk of plastic pollution in terms of quantity. Their size distribution has been shown to follow a power-law for larger fragments. This work introduces a novel model inspired by raindrop formation, incorporating local oceanographic processes and fragment size, aiming to improve the understanding and prediction of plastic fragmentation in marine environments. Particles can fragment when they reach a certain size, or when shear forces become too strong. Plastic aging’s effect on size distribution is also investigated.
NUAM-Net: A Novel Underwater Image Enhancement Attention Mechanism Network
Authors: Wen Zhang , Zhao Yikang , Gao Feng , Su Hao , Rao Yuan , Dong Junyu
Published: July 2024

Vision-based underwater exploration is crucial for marine research. However, the degradation of underwater images due to light attenuation and scattering poses a significant challenge. This results in the poor visual quality of underwater images and impedes the development of vision-based underwater exploration systems. Recent popular learning-based Underwater Image Enhancement (UIE) methods address this challenge by training enhancement networks with annotated image pairs, where the label image is manually selected from the reference images of existing UIE methods since the groundtruth of underwater images do not exist. Nevertheless, these methods encounter uncertainty issues stemming from ambiguous multiple-candidate references. Moreover, they often suffer from local perception and color perception limitations, which hinder the effective mitigation of wide-range underwater degradation. This paper proposes a novel NUAM-Net (Novel Underwater Image Enhancement Attention Mechanism Network) that addresses these limitations. NUAM-Net leverages a probabilistic training framework, measuring enhancement uncertainty to learn the UIE mapping from a set of ambiguous reference images. By extracting features from both the RGB and LAB color spaces, our method fully exploits the fine-grained color degradation clues of underwater images. Additionally, we enhance underwater feature extraction by incorporating a novel Adaptive Underwater Image Enhancement Module (AUEM) that incorporates both local and long-range receptive fields. Experimental results on the well-known UIEBD benchmark demonstrate that our method significantly outperforms popular UIE methods in terms of PSNR while maintaining a favorable Mean Opinion Score. The ablation study also validates the effectiveness of our proposed method.
Short-Term Photovoltaic Power Prediction Based on a Digital Twin Model
Authors: Xiang Chuan , Li Bohan , Shi Pengfei , Yang Tiankai , Han Bing
Published: July 2024

Due to the influence of meteorological conditions, shipboard photovoltaic (PV) systems have problems such as large fluctuation and inaccurate prediction of the output power. In this paper, a short-term PV power prediction method based on a novel digital twin (DT) model and BiLSTM is proposed. Firstly, a PV mechanism model and a data-driven model were established, in which the data-driven model was updated iteratively in real time using the sliding time window update method; then, these two models were converged to construct a PV DT model according to the DS evidence theory. Secondly, a BiLSTM model was built to make short-term predictions of the PV power using the augmented dataset of the DT model as an input. Finally, the method was tested and verified by experiments and further compared with main PV prediction methods. The research results indicate the following: firstly, the absolute error of the DT model was smaller than that of the mechanism model and the data-driven model, being as low as 5.62 W after the data update of the data-driven model; thus, the DT model realized data augmentation and high fidelity. Secondly, compared to several main PV prediction models, the PV DT model combined with BiLSTM had the lowest RMSE, MAE, and MAPE; the best followability; and the smallest absolute error under different weather conditions, which was especially obvious under cloudy weather conditions. In summary, the method can accurately predict the shipboard PV power, which has great theoretical significance and application value for improving the economy and reliability of solar ship operation.
Transcriptomic and Metabolomic Analyses of Soybean Protein Isolate on Monascus Pigments and Monacolin K Production
Authors: Qin Xueling , Han Haolan , Zhang Jiayi , Xie Bin , Zhang Yufan , Liu Jun , Dong Weiwei , Hu Yuanliang , Yu Xiang , Feng Yanli
Published: July 2024

Monascus pigments (MPs) and monacolin K (MK) are important secondary metabolites produced by Monascus spp. This study aimed to investigate the effect of soybean protein isolate (SPI) on the biosynthesis of MPs and MK based on the analysis of physiological indicators, transcriptomes, and metabolomes. The results indicated that the growth, yellow MPs, and MK production of Monascus pilosus MS-1 were significantly enhanced by SPI, which were 8.20, 8.01, and 1.91 times higher than that of the control, respectively. The utilization of a nitrogen source, protease activity, the production and utilization of soluble protein, polypeptides, and free amino acids were also promoted by SPI. The transcriptomic analysis revealed that the genes mokA, mokB, mokC, mokD, mokE, mokI, and mokH which are involved in MK biosynthesis were significantly up-regulated by SPI. Moreover, the glycolysis/gluconeogenesis, pyruvate metabolism, fatty acid degradation, tricarboxylic acid (TCA) cycle, and amino acid metabolism were effectively up-regulated by SPI. The metabolomic analysis indicated that metabolisms of amino acid, lipid, pyruvate, TCA cycle, glycolysis/gluconeogenesis, starch and sucrose, and pentose phosphate pathway were significantly disturbed by SPI. Thus, MPs and MK production promoted by SPI were mainly attributed to the increased biomass, up-regulated gene expression level, and more precursors and energies.
Association of Peripheral Vestibular Disorder with Diabetes: A Population-Based Study
Authors: Yang Tzong-Hann , Chen Chao-Hung , Cheng Yen-Fu , Lin Herng-Ching , Chen Chin-Shyan
Published: July 2024

Background: This study aimed to investigate the association of peripheral vestibular disorders with type 1 and type 2 diabetes using a population-based dataset. Methods: The data for this study were obtained from Taiwan’s Longitudinal Health Insurance Database 2010. The sample consisted of 150,916 patients who were newly diagnosed with peripheral vestibular disorders as cases and 452,748 propensity-score-matching controls without peripheral vestibular disorders. We utilized multivariate logistic regression models to quantitatively evaluate the association between peripheral vestibular disorders and diabetes while considering factors such as sex, age, geographic location, monthly income, urbanization level of the patient’s residence, coronary heart disease, hypertension, and hyperlipidemia. Results: The chi-squared test indicates that diabetes was more common in the peripheral vestibular disorder group compared to controls (20.6% vs. 15.1%, p < 0.001). Of all sampled patients, the adjusted odds ratio for diabetes was 1.597 (95% CI = 1.570~1.623) for those with peripheral vestibular disorders when compared to controls, while patients with Ménière’s disease, benign paroxysmal positional vertigo, unilateral vestibulopathy, and other peripheral vestibular disorders had respective adjusted odds ratios of diabetes at 1.566 (95% CI = 1.498~1.638), 1.677 (95% CI = 1.603~1.755), 1.592 (95% CI = 1.504~1.685), and 1.588 (95% CI = l.555~1.621) in comparison to controls. Conclusions: Our research has revealed an association between diabetes and an increased susceptibility to peripheral vestibular disorders.
Community Life Circle, Neighbourly Interaction, and Social Cohesion: Does Community Space Use Foster Stronger Communities?
Authors: Jiang Min , Hu Jiaqi , Gao Xiaofeng
Published: July 2024

Neighbourhood social cohesion has emerged as a prominent concern in urban governance worldwide. As the primary domain of residents’ daily activities, the community life circle contributes to neighbourly interaction and social cohesion. This study investigates the role of space use within the community life circle in promoting social cohesion in Chongqing, China. Through an in-depth activity diary survey and structural equation model analysis, we empirically examine the interplay between community space use, neighbourly interaction, and social cohesion. Our findings suggest that the 15 min community life circle plays a crucial role in residents’ daily lives, particularly among senior residents. However, the contribution of community space use to social cohesion is fully mediated by neighbourly interaction. Furthermore, not all patterns of community space use contribute to social cohesion equally; space use engaged with diverse companions on workdays significantly enhances neighbourly interaction and social cohesion. The study enriches the existing literature by deepening our understanding of the role of the community life circle in fostering socially cohesive and sustainable neighbourhoods.