bluuebunny/crossref_metadata_2025 huggingface dataset:
To Predict, Prevent, and Manage Post-Traumatic Stress Disorder (PTSD): A Review of Pathophysiology, Treatment, and Biomarkers
Authors: Al Jowf Ghazi I. , Ahmed Ziyad T. , Reijnders Rick A. , de Nijs Laurence , Eijssen Lars M. T.
Published: March 2023

Post-traumatic stress disorder (PTSD) can become a chronic and severely disabling condition resulting in a reduced quality of life and increased economic burden. The disorder is directly related to exposure to a traumatic event, e.g., a real or threatened injury, death, or sexual assault. Extensive research has been done on the neurobiological alterations underlying the disorder and its related phenotypes, revealing brain circuit disruption, neurotransmitter dysregulation, and hypothalamic–pituitary–adrenal (HPA) axis dysfunction. Psychotherapy remains the first-line treatment option for PTSD given its good efficacy, although pharmacotherapy can also be used as a stand-alone or in combination with psychotherapy. In order to reduce the prevalence and burden of the disorder, multilevel models of prevention have been developed to detect the disorder as early as possible and to reduce morbidity in those with established diseases. Despite the clinical grounds of diagnosis, attention is increasing to the discovery of reliable biomarkers that can predict susceptibility, aid diagnosis, or monitor treatment. Several potential biomarkers have been linked with pathophysiological changes related to PTSD, encouraging further research to identify actionable targets. This review highlights the current literature regarding the pathophysiology, disease development models, treatment modalities, and preventive models from a public health perspective, and discusses the current state of biomarker research.
Unfolding Individual Domains of BmrA, a Bacterial ABC Transporter Involved in Multidrug Resistance
Authors: Oepen Kristin , Mater Veronika , Schneider Dirk
Published: March 2023

The folding and stability of proteins are often studied via unfolding (and refolding) a protein with urea. Yet, in the case of membrane integral protein domains, which are shielded by a membrane or a membrane mimetic, urea generally does not induce unfolding. However, the unfolding of α-helical membrane proteins may be induced by the addition of sodium dodecyl sulfate (SDS). When protein unfolding is followed via monitoring changes in Trp fluorescence characteristics, the contributions of individual Trp residues often cannot be disentangled, and, consequently, the folding and stability of the individual domains of a multi-domain membrane protein cannot be studied. In this study, the unfolding of the homodimeric bacterial ATP-binding cassette (ABC) transporter Bacillus multidrug resistance ATP (BmrA), which comprises a transmembrane domain and a cytosolic nucleotide-binding domain, was investigated. To study the stability of individual BmrA domains in the context of the full-length protein, the individual domains were silenced by mutating the existent Trps. The SDS-induced unfolding of the corresponding constructs was compared to the (un)folding characteristics of the wild-type (wt) protein and isolated domains. The full-length variants BmrAW413Y and BmrAW104YW164A were able to mirror the changes observed with the isolated domains; thus, these variants allowed for the study of the unfolding and thermodynamic stability of mutated domains in the context of full-length BmrA.
The Physio-Pathological Role of Group I Metabotropic Glutamate Receptors Expressed by Microglia in Health and Disease with a Focus on Amyotrophic Lateral Sclerosis
Authors: Balbi Matilde , Bonanno Giambattista , Bonifacino Tiziana , Milanese Marco
Published: March 2023

Microglia cells are the resident immune cells of the central nervous system. They act as the first-line immune guardians of nervous tissue and central drivers of neuroinflammation. Any homeostatic alteration that can compromise neuron and tissue integrity could activate microglia. Once activated, microglia exhibit highly diverse phenotypes and functions related to either beneficial or harmful consequences. Microglia activation is associated with the release of protective or deleterious cytokines, chemokines, and growth factors that can in turn determine defensive or pathological outcomes. This scenario is complicated by the pathology-related specific phenotypes that microglia can assume, thus leading to the so-called disease-associated microglia phenotypes. Microglia express several receptors that regulate the balance between pro- and anti-inflammatory features, sometimes exerting opposite actions on microglial functions according to specific conditions. In this context, group I metabotropic glutamate receptors (mGluRs) are molecular structures that may contribute to the modulation of the reactive phenotype of microglia cells, and this is worthy of exploration. Here, we summarize the role of group I mGluRs in shaping microglia cells’ phenotype in specific physio-pathological conditions, including some neurodegenerative disorders. A significant section of the review is specifically focused on amyotrophic lateral sclerosis (ALS) since it represents an entirely unexplored topic of research in the field.
The Genetic Basis of Future Pharmacological Strategies for the Management of Comorbid Obesity and Depression: A Scoping Review
Authors: Rajkumar Ravi Philip
Published: March 2023

Depression and obesity are highly comorbid with one another, with evidence of bidirectional causal links between each disorder and a shared biological basis. Genetic factors play a major role in influencing both the occurrence of comorbid depression and obesity, their courses, and their response to existing treatments. The current paper is a scoping review of studies that have evaluated the contribution of specific genetic variants to the comorbidity between obesity and depression. Based on a search of the PubMed and EMBASE databases, 28 studies were included in this review, covering 54 candidate genes. Positive associations were identified for 14 genetic loci (AKR1C2, APOA5, COMT, DAT1, FTO, KCNE1, MAOA, MC4R, MCHR2, NPY2R, NR3C1, Ob, PCSK9, and TAL1). Replicated findings across two or more independent samples were observed for the FTO and MC4R genes. Many of these gene products represent novel molecular targets for the pharmacological management of obesity that interact with each other and are not pharmacologically influenced by existing anti-obesity or antidepressant medications. The implications of these associations for future drug development are discussed, with an emphasis on recent evidence on the polygenic architecture of comorbid depression and obesity and on a precision-medicine approach to these conditions.
Quickening Data-Aware Conformance Checking through Temporal Algebras
Authors: Bergami Giacomo , Appleby Samuel , Morgan Graham
Published: March 2023

A temporal model describes processes as a sequence of observable events characterised by distinguishable actions in time. Conformance checking allows these models to determine whether any sequence of temporally ordered and fully-observable events complies with their prescriptions. The latter aspect leads to Explainable and Trustworthy AI, as we can immediately assess the flaws in the recorded behaviours while suggesting any possible way to amend the wrongdoings. Recent findings on conformance checking and temporal learning lead to an interest in temporal models beyond the usual business process management community, thus including other domain areas such as Cyber Security, Industry 4.0, and e-Health. As current technologies for accessing this are purely formal and not ready for the real world returning large data volumes, the need to improve existing conformance checking and temporal model mining algorithms to make Explainable and Trustworthy AI more efficient and competitive is increasingly pressing. To effectively meet such demands, this paper offers KnoBAB, a novel business process management system for efficient Conformance Checking computations performed on top of a customised relational model. This architecture was implemented from scratch after following common practices in the design of relational database management systems. After defining our proposed temporal algebra for temporal queries (xtLTLf), we show that this can express existing temporal languages over finite and non-empty traces such as LTLf. This paper also proposes a parallelisation strategy for such queries, thus reducing conformance checking into an embarrassingly parallel problem leading to super-linear speed up. This paper also presents how a single xtLTLf operator (or even entire sub-expressions) might be efficiently implemented via different algorithms, thus paving the way to future algorithmic improvements. Finally, our benchmarks highlight that our proposed implementation of xtLTLf (KnoBAB) outperforms state-of-the-art conformance checking software running on LTLf logic.
Collectable Single Pure-Pd Metal Membrane with High Strength and Flexibility Prepared through Electroplating for Hydrogen Purification
Authors: Endo Naruki , Kaneko Yumi , Dezawa Norikazu , Komo Yasuhiro , Higuchi Masanobu
Published: March 2023

Among the various film preparation methods, electroplating is one of the simplest and most economical methods. However, it is challenging to collect a dense single Pd film through plating, owing to the accumulation of stress in the film during the process. Therefore, the characteristics of a single plated film have not been clearly identified, although pure Pd is widely used in metallic-hydrogen-purification membranes. In this study, stress concentration in film during preparation was reduced by optimizing the plating process, and a dense single flat film was successfully collected. No impurities were detected. Thus, a high-purity Pd film was prepared. Its surface texture was found to be significantly different from that of the rolled film, and several approximately 5 μm sized aggregates were observed on the surface. The plated film is reported to have mechanical properties superior to those of the rolled film, with twice the displacement and four times the breaking point strength. The hydrogen permeabilities of the plated film (5.4 × 10−9–1.1 × 10−8 mol·m−1·s−1·Pa−1/2 at 250–450 °C) were comparable to those of the rolled and reported films, indicating that the surface texture does not have a strong effect on hydrogen permeability. The results of this study promote the practical use of Pd-based membranes through electroplating.
Long-Term Adverse Effects of Mild COVID-19 Disease on Arterial Stiffness, and Systemic and Central Hemodynamics: A Pre-Post Study
Authors: Podrug Mario , Koren Pjero , Dražić Maras Edita , Podrug Josip , Čulić Viktor , Perissiou Maria , Bruno Rosa Maria , Mudnić Ivana , Boban Mladen , Jerončić Ana
Published: March 2023

COVID-19-associated vascular disease complications are primarily associated with endothelial dysfunction; however, the consequences of disease on vascular structure and function, particularly in the long term (>7 weeks post-infection), remain unexplored. Individual pre- and post-infection changes in arterial stiffness as well as central and systemic hemodynamic parameters were measured in patients diagnosed with mild COVID-19. As part of in-laboratory observational studies, baseline measurements were taken up to two years before, whereas the post-infection measurements were made 2–3 months after the onset of COVID-19. We used the same measurement protocol throughout the study as well as linear and mixed-effects regression models to analyze the data. Patients (N = 32) were predominantly healthy and young (mean age ± SD: 36.6 ± 12.6). We found that various parameters of arterial stiffness and central hemodynamics—cfPWV, AIx@HR75, and cDBP as well as DBP and MAP—responded to a mild COVID-19 disease. The magnitude of these responses was dependent on the time since the onset of COVID-19 as well as age (pregression_models ≤ 0.013). In fact, mixed-effects models predicted a clinically significant progression of vascular impairment within the period of 2–3 months following infection (change in cfPWV by +1.4 m/s, +15% in AIx@HR75, approximately +8 mmHg in DBP, cDBP, and MAP). The results point toward the existence of a widespread and long-lasting pathological process in the vasculature following mild COVID-19 disease, with heterogeneous individual responses, some of which may be triggered by an autoimmune response to COVID-19.
The Potential Roles of Ferroptosis in Pathophysiology and Treatment of Musculoskeletal Diseases—Opportunities, Challenges, and Perspectives
Authors: Hu Yunxiang , Wang Yufei , Liu Sanmao , Wang Hong
Published: March 2023

Ferroptosis is different from other forms of cell death, such as apoptosis, autophagy, pyroptosis, and cuproptosis, mainly involving iron metabolism and lipid peroxidation. Ferroptosis plays an important role in various disease, such as malignant tumors, neuron-degenerative diseases, and cardiovascular diseases, and has become the focus of current research. Both iron overload and lipid peroxide accumulation contribute to the occurrence, development, and treatment of musculoskeletal diseases, such as osteoporosis, osteoarthritis, osteosarcoma, intervertebral disc degeneration, and spinal cord injury. For a better understanding of the potential roles ferroptosis may play in pathophysiology and treatment of common musculoskeletal disorders, this article briefly reviewed the relationship and possible mechanisms. Through an investigation of ferroptosis’ role in musculoskeletal diseases’ occurrence, development, and treatment, ferroptosis could offer new opportunities for clinical diagnosis and treatment.
Comparative Study of Dental Custom CAD-CAM Implant Abutments and Dental Implant Stock Abutments
Authors: Târtea Daniel Adrian , Ionescu Mihaela , Manolea Horia Octavian , Mercuț Veronica , Obădan Eugenia , Amărăscu Marina Olimpia , Mărășescu Petre Costin , Dăguci Luminița , Popescu Sanda Mihaela
Published: March 2023

The implementation of CAD-CAM systems in dentistry has significantly influenced the evolution of dental implantology and implant-supported prosthetics within the past three decades. Implant-supported prostheses are comfortable and aesthetic. The prosthetic abutment has also faced a rapid design evolution, from the individualization of standard stock abutments offered by various manufacturers to a modern customization process using CAD-CAM technology. This paper presents a comparative study between 20 dental custom CAD-CAM implant abutments and 20 dental implant stock abutments, based on a set of measurements performed on the digital casts obtained from 24 cases of prosthetic rehabilitation on implants. The statistical analysis (Mann–Whitney U test) revealed significant differences between these two types of abutments: the incisal margin line diameter dimensions for custom abutments were significantly improved compared to standard abutments at the cervical level (U = 343.00, z = 3.868, p < 0.0005) and the incisal/occlusal level (U = 352.00, z = 4.112, p < 0.0005), while the inclination angle of the custom abutments relative to the 0-axis was significantly smaller than that of standard abutments (U = 115.50, z = −2.286, p = 0.022). The use of custom abutments leads to an increase in the final size of the abutment, an improvement in the retention of the prosthetic work, and reduces the angulation of the abutment in relation to the implant axis, thus decreasing the risk of unscrewing or fracturing the dental screw.
Dyslipidemia Exacerbates Meibomian Gland Dysfunction: A Systematic Review and Meta-Analysis
Authors: Tomioka Yasufumi , Kitazawa Koji , Yamashita Yohei , Numa Kohsaku , Inomata Takenori , Hughes Jun-Wei B. , Soda Rina , Nakamura Masahiro , Suzuki Tomo , Yokoi Norihiko , Sotozono Chie
Published: March 2023

Dry eye is a multifactorial and common age-related ocular surface disease. Dyslipidemia has been reported to be involved in meibomian gland dysfunction (MGD). However, it has not been clearly identified which lipid abnormality is responsible for MGD. In this systematic review and meta-analysis, we discuss how lipid profile changes with aging is responsible for MGD development. Methods. An article search was performed in PubMed, EMBASE, and Web of Science. Eleven studies involving dyslipidemia in patients with MGD were identified. Five out of eleven studies were further analyzed with meta-analysis. The preferred reporting items for Systematic Reviews and Meta-Analyses (PRISMA) reporting guidelines were followed. Study-specific estimates (prevalence of dyslipidemia in MGD patients) were combined using one-group meta-analysis in a random-effects model. Results. Meta-analysis revealed that high total cholesterol (TC) and high triglycerides (TG) were significantly associated with MGD prevalence, with odds ratios of 5.245 (95% confidence interval [CI]: 1.582–17.389; p < 0.001) and 3.264 (95% CI: 1.047–10.181; p < 0.001), respectively, but high low-density lipoprotein (LDL) and low high-density lipoprotein (HDL) were not identified. Systematic review found that the percentage of MGD patients with TC ≥ 200 mg/dL ranged from 20.0–77.6%, TG ≥ 150 mg/dL ranged from 8.3–89.7%, whereas, in the aged-match-adjusted controls, TC range of 200 mg/dL or higher and TG range of 150 mg/dL was 6.1–45.1% and 1.1–47.8%, respectively. The severity of MGD was higher with dyslipidemia. Conclusion. Dyslipidemia and higher TC and TG are significant risk factors for MGD.
Radiation Workers and Risk Perceptions: Low Dose Radiation, Nuclear Power Production, and Small Modular Nuclear Reactors
Authors: Hurlbert Margot , Shasko Larissa , Condor Jose , Landrie-Parker Dazawray
Published: March 2023

People’s affective response in relation to radiation is important in their risk perceptions of low-dose radiation (LDR), medical interventions involving LDR, and acceptance of nuclear power production. Risk perception studies generally relate to the health field of LDR or nuclear power. This study combines risk perceptions and acceptance of both. While acceptance by those with an understanding of radiation is demonstrated in focus groups, survey results disproved this correlation. Emotional response to the word radiation together with greater perceptions of risk to X-rays, were predictors of acceptance of nuclear power production.
Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
Authors: Zhang Yuan , He Kai , Guo Xuhao , Jiang Jia , Qian Le , Xu Jianqiang , Che Zhiping , Huang Xiaobo , Liu Shengming
Published: March 2023

Fusarium pseudograminearum has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) was used to determine the different mechanisms of action of four frequently used fungicides including carbendazim, pyraclostrobin, tebuconazole, and phenamacril on F. pseudograminearum. In brief, 381, 1896, 842, and 814 differentially expressed genes (DEGs) were identified under the carbendazim, pyraclostrobin, tebuconazole, and phenamacril treatments, respectively. After the joint analysis, 67 common DEGs were obtained, and further functional analysis showed that the ABC transported pathway was significantly enriched. Moreover, FPSE_04130 (FER6) and FPSE_11895 (MDR1), two important ABC multidrug transporter genes whose expression levels simultaneously increased, were mined under the different treatments, which unambiguously demonstrated the common effects. In addition, Mfuzz clustering analysis and WGCNA analysis revealed that the core DEGs are involved in several critical pathways in each of the four treatment groups. Taken together, these genes may play a crucial function in the mechanisms of F. pseudograminearum‘s response to the fungicides stress.
Effects of Failure on California Sea Lion (Zalophus californianus) Gameplay Strategies and Interest in a Cognitive Task: Implications for Cognitive Enrichment in Pinnipeds
Authors: Roberts Danielle L. , Eskelinen Holli C. , Winship Kelley A. , Ramos Amber M. , Xitco Mark J.
Published: March 2023

Cognitive enrichment for professionally managed species has become more prevalent in recent years in both zoological and research settings and has been encouraged as a means of welfare enhancement. However, the task’s difficulty must be specifically tailored as it can impact the successful nature of the sessions, as tasks that are too simple or difficult may not be perceived as enriching by the animals. While pinnipeds are common in zoos, aquariums, and research facilities, few studies have explored the use of cognitively challenging enrichment in this species, and the level of difficulty and presence of failure on animal success and engagement in this type of session has not been assessed. In this study, gameplay strategies during computerized enrichment sessions were evaluated before and after a game that introduced failure, or the loss of opportunity to complete a level for a reward after an incorrect movement. Interest in participation during the session, measured as the latency without contact, was also tested as a proxy for this enrichment’s effect on welfare. When incorrect movements resulted in a short pause and removed the opportunity to finish individual levels for a reward, all three sea lions tested significantly reduced the amount of time spent on each of several strategies they employed, but significantly increased the number of button presses per strategy, suggesting the animals focused on more precise movements as their proficiency improved. Two sea lions also showed a significant decline in latency without contact following the introduction of failure in the form of a single opportunity to complete a task for a reward after previously having unlimited opportunities, while one maintained a low latency without contact across both test conditions. The results suggest that more cognitively challenging tasks incorporating failure did not cause a reduction in gameplay performance and session interest in sea lions. Individual variation was also noted in strategy use, emphasizing the importance of evaluating the individual in terms of enrichment provision.
Ascertaining the Knowledge of the General Public and Stakeholders in the Forestry Sector to Invasive Alien Species—A Pan-European Study
Authors: Bălăcenoiu Flavius , Japelj Anže , Bernardinelli Iris , Castagneyrol Bastien , Csóka György , Glavendekić Milka , Hoch Gernot , Hrasovec Boris , Krajter Ostoić Silvija , Paulin Marton , Williams David , Witters Johan , de Groot Maarten
Published: March 2023

Against the background of the phenomenon of globalisation, which has increasingly intensified in recent decades, invasive alien species (IAS) have led to biological invasions that have resulted in multiple negative effects on economies, human health, and especially on the environment. In order to control invasive alien species, preventive actions are considered the most effective methods. In this context, society can actively participate in the process of early detection and preventing the spread of these organisms, but there is a need to raise public awareness. In order for this process to take place in the most efficient way, it is necessary to initially evaluate the knowledge of the general public to IAS. Through a questionnaire that was circulated in ten European countries and had over two thousand respondents, this study aimed to investigate the level of knowledge of some stakeholders in the forestry sector regarding IAS. The results showed that a vast majority of respondents who participated in the study had heard about IAS and provided a correct definition of these organisms. Most of the respondents in this study heard for the first time about IAS from school, the Internet, or journal articles. Data analysis also showed that stakeholders in the forestry sector (foresters, forest owners, and members of environmental NGOs) were more likely than the other respondents to be aware of the impact of IAS. The results of this study offer an insight to researchers and decision makers assessing the differences of opinion regarding invasive alien species, and the necessary steps that could be adopted in the process of raising awareness in society.
Rollover Stability of Heavy-Duty AGVs in Turns Considering Variation in Friction Coefficient
Authors: Fu Weijie , Wang Xinyu , Zhang Xinming
Published: March 2023

This study analysed the impact of turning rate, the centre of mass height, and road adhesion coefficient on the rollover stability of heavy-duty automated guided vehicles (AGVs) using a multi-body dynamics simulation model. The lateral deflection angle of the centre of mass was used as a metric to evaluate the rollover behaviour of the AGVs, and the results were obtained quantitatively. The findings showed that the turning rate had the largest impact on AGV rollover stability, followed by the centre of mass height, while the road adhesion coefficient had the least impact. Despite having the lowest impact, the road adhesion coefficient was one of the key factors contributing to AGV slippage, and severe slippage could easily lead to rollover incidents. To further evaluate the rollover behaviour of the AGVs, a lateral-load transfer rate (LTR) index was derived from the change in wheel load generated by the lateral tilt angle during steering. The range of LTR values was determined for different ranges of turning rate, the centre of mass height, and road adhesion coefficient. The results indicated that the range of LTR values for turning rate was 0.23–0.45, for the centre of mass height was 0.323–0.393, and the minimum value of 0.337 was obtained for a road adhesion coefficient of 0.6.
Evaluation of Marginal and Internal Fit of Ceramic Laminate Veneers Fabricated with Five Intraoral Scanners and Indirect Digitization
Authors: Al-Dwairi Ziad N. , Al-Sardi Moataz , Goodacre Brian J. , Goodacre Charles J. , Al Hamad Khaled Q. , Özcan Mutlu , Al-Haj Husain Nadin , Baba Nadim Z.
Published: March 2023

The long-term success of ceramic laminate veneers (CLVs) is influenced by the marginal and internal fit of the restorations. However, studies comparing the fit of CLVs using different intraoral scanners or the indirect digitization technique are lacking. The purpose of this study was therefore to assess the marginal and internal fit of CAD/CAM-milled CLVs using different intraoral scanners and the indirect digitalization technique. An ivorine typodont maxillary left-central incisor was prepared; the tooth and the neighboring teeth were scanned and used as a template to print ninety 3D partial models. Thereafter, ceramic laminate veneers (CLVs) (N = 90) were milled from IPS-Emax CAD blocks and divided into six equal groups (15 specimens each) according to the type of intraoral scanner (IOS), as follows: Omnicam IOS, SC3600 IOS, Trios 3 IOS, Emerald IOS, I500 IOS. Fifteen further CLVs were fabricated using the conventional indirect digitalization technique. After cementation on the resin dies and embedding in clear epoxy resin, specimens were sectioned inciso-gingivally and mesio-distally. At the incisal and cervical positions, the marginal discrepancy was measured and evaluated in addition to the internal gap at six locations using SEM (200×). Differences between gap measurements among the six groups were determined using ANOVA. Games–Howell multiple comparisons for homogenous variances and LSD multiple comparisons for non-homogenous variances were used with 95% confidence intervals. The significance level was set at 0.05. The lowest mean absolute marginal gap at the incisal margins (AMGI) was recorded for Omnicam group (203.28 ± 80.14) µm, while the highest mean absolute marginal gap at the cervical margins (AMGC) was recorded for Omnicam group (147.16 ± 59.78) µm. The mean AMGC was reported to be significantly different between the conventional technique (146.75 ± 38.43) µm and Trios 3 (91.86 ± (35.51) µm; p = 0.001) and between Emerald (112.37 ± (50.31) µm; p = 0.042) and I500 (86.95 ± (41.55) µm; p < 0.001). The mean MGI was found to be significantly different between the conventional technique (114.11 ± (43.45) µm and I500 group (186.99 ± (73.84) µm) only (p = 0.035). However, no significant differences were found in the mean MGI between all types of IOSs. The means of AMG and MG were significantly different at incisal or cervical areas between the conventional technique and IOSs and within the scanner groups (p > 0.05). Marginal gaps were higher in the incisal region compared to the cervical region with both the indirect digitization technique and the IOSs. Ceramic laminate veneers (CLVs) fabricated using IOSs produced overall internal and marginal fit adaptation results comparable to CLVs fabricated from the indirect digitalization method, and both techniques produced clinically acceptable results.
Plasma Electrolytic Oxidation of Titanium in Ni and Cu Hydroxide Suspensions towards Preparation of Electrocatalysts for Urea Oxidation
Authors: Wala Marta , Łubiarz Dorota , Waloszczyk Natalia , Simka Wojciech
Published: March 2023

The increasing climate crisis requires an improvement in renewable energy technologies. One of them are fuel cells, devices that are capable of generating electricity directly from the chemical reaction that is taking place inside of them. Despite the advantages of these solutions, a lack of the appropriate materials is holding them back from commercialization. This research shows preliminary results from a simple way to prepare black TiO2 coatings, doped with Cu or Ni using the plasma electrolytic oxidation process, which can be used as anodes in urea-fueled fuel cells. They show activity toward urea oxidation, with a maximum current density of 130 μA cm−2 (@1 V vs. Hg|HgO) observed for Cu-enhanced TiO2 and low potential of only 0.742 V (Vs Hg|HgO) required for 50 μA cm−2 for Ni-enhanced TiO2. These results demonstrate how the PEO process can be used for the preparation of TiO2-based doped materials with electrocatalytic properties toward urea electrooxidation.
Scalar Variance and Scalar Correlation for Functional Data
Authors: Urbano-Leon Cristhian Leonardo , Escabias Manuel , Ovalle-Muñoz Diana Paola , Olaya-Ochoa Javier
Published: March 2023

In Functional Data Analysis (FDA), the existing summary statistics so far are elements in the Hilbert space L2 of square-integrable functions. These elements do not constitute an ordered set; therefore, they are not sufficient to solve problems related to comparability such as obtaining a correlation measurement or comparing the variability between two sets of curves, determining the efficiency and consistency of a functional estimator, among other things. Consequently, we present an approach of coherent redefinition of some common summary statistics such as sample variance, sample covariance and correlation in Functional Data Analysis (FDA). Regarding variance, covariance and correlation between functional data, our summary statistics lead to numbers instead of functions which is helpful for solving the aforementioned problems. Furthermore, we briefly discuss the functional forms coherence of some statistics already present in the FDA. We formally enumerate and demonstrate some properties of our functional summary statistics. Then, a simulation study is presented briefly, with evidence of the consistency of the proposed variance. Finally, we present the implementation of our statistics through two application examples.
An Analysis of PISA 2018 Mathematics Assessment for Asia-Pacific Countries Using Educational Data Mining
Authors: Bayirli Ezgi Gülenç , Kaygun Atabey , Öz Ersoy
Published: March 2023

The purpose of this paper is to determine the variables of high importance affecting the mathematics achievement of the students of 12 Asia-Pacific countries participating in the Program for International Student Assessment (PISA) 2018. For this purpose, we used random forest (RF), logistic regression (LR) and support vector machine (SVM) models to classify student achievement in mathematics. The variables affecting the student achievement in mathematics were examined by the feature importance method. We observed that the variables with the highest importance for all of the 12 Asia-Pacific countries we considered are the educational status of the parents, having access to educational resources, age, the time allocated to weekly lessons, and the age of starting kindergarten. Then we applied two different clustering analysis by using the variable importance values and socio-economic variables of these countries. We observed that Korea, Japan and Taipei form one group of Asia-Pacific countries, while Thailand, China, Indonesia, and Malaysia form another meaningful group in both clustering analyses. The results we obtained strongly suggest that there is a quantifiable relationship between the educational attainment and socio-economic levels of these 12 Asia-Pacific countries.
Correlation between Morphological Characteristics of Macular Edema and Visual Acuity in Young Patients with Idiopathic Intermediate Uveitis
Authors: Iannetti Ludovico , Scarinci Fabio , Alisi Ludovico , Armentano Marta , Sampalmieri Lorenzo , La Cava Maurizio , Gharbiya Magda
Published: March 2023

Background and Objectives: Macular edema (ME) is a common complication of intermediate uveitis (IU). It is often responsible for a decrease in visual acuity (VA). Three distinct patterns of macular edema have been described in intermediate uveitis, namely, cystoid macular edema (CME), diffuse macular edema (DME), and serous retinal detachment (SRD). The current study aims to describe the characteristics of macular edema in young patients with idiopathic intermediate uveitis and to correlate its features with VA using spectral domain optical coherence tomography (SD-OCT). Materials and Methods: A total of 27 eyes from 18 patients with idiopathic IU complicated by ME were included in this retrospective study. All patients underwent SD-OCT; data were gathered at the onset of ME. Best-corrected VA (BCVA) was correlated with the morphological features of ME. Results: BCVA was negatively correlated with Ellipsoid Zone (EZ) disruption (p = 0.00021), cystoid pattern (p = 0.00021), central subfield thickness (CST) (p < 0.001), and serous retinal detachment (0.037). Conclusions: In ME secondary to idiopathic IU, VA negatively correlates with Ellipsoid Zone disruption and increases in CST. Moreover, vision is influenced by the presence of cysts in the inner nuclear and outer nuclear layers and by the neuroepithelium detachment.
Autologous Collagen-Induced Chondrogenesis: From Bench to Clinical Development
Authors: Chun You Seung , Kim Seon Ae , Kim Yun Hwan , Lee Joong Hoon , Shetty Asode Ananthram , Kim Seok Jung
Published: March 2023

Microfracture is a common technique that uses bone marrow components to stimulate cartilage regeneration. However, the clinical results of microfracture range from poor to good. To enhance cartilage healing, several reinforcing techniques have been developed, including porcine-derived collagen scaffold, hyaluronic acid, and chitosan. Autologous collagen-induced chondrogenesis (ACIC) is a single-step surgical technique for cartilage regeneration that combines gel-type atelocollagen scaffolding with microfracture. Even though ACIC is a relatively new technique, literature show excellent clinical results. In addition, all procedures of ACIC are performed arthroscopically, which is increasing in preference among surgeons and patients. The ACIC technique also is called the Shetty–Kim technique because it was developed from the works of A.A. Shetty and S.J. Kim. This is an up-to-date review of the history of ACIC.
Fatigue Is Associated with Anxiety and Lower Health-Related Quality of Life in Patients with Inflammatory Bowel Disease in Remission
Authors: Stroie Tudor , Preda Carmen , Meianu Corina , Istrătescu Doina , Manuc Mircea , Croitoru Adina , Gheorghe Liana , Gheorghe Cristian , Diculescu Mircea
Published: March 2023

Background and Objectives: Inflammatory bowel diseases (IBD) are chronic conditions with an unpredictable course and a remitting–relapsing evolution. Fatigue is a frequent complaint in patients with IBD, affecting approximately half of the newly diagnosed patients with IBD. The aim of this study was to analyze fatigue in patients with IBD in remission. Materials and Methods: One hundred nineteen consecutive outpatients diagnosed with IBD for over 3 months that were in corticosteroid-free clinical and biochemical remission at the time of assessment were included in this cross-sectional study. Out of them, 72 (60.5%) were male; the median age was 39 years (IQR 30–47). Seventy-seven patients (64.7%) were diagnosed with Crohn’s disease and forty-two (35.3%) with ulcerative colitis, with a median disease duration of 6 years (IQR 2–10). Fatigue, health-related quality of life (HR-QoL), anxiety and depression were evaluated using the following self-administered questionnaires: FACIT Fatigue, IBDQ 32 and HADS. Results: The mean FACIT-Fatigue score was 41.6 (SD ± 8.62), and 38.7% of patients were revealed as experiencing fatigue when a cut-off value of 40 points was used. The mean IBDQ 32 score was 189.4 (SD ± 24.1). Symptoms of anxiety and depression were detected in 37% and 21% of the patients, respectively. In the multivariate analysis, fatigue was significantly associated with lower HR-QoL (OR 2.21, 95% CI: 1.42–3.44, p < 0.001), symptoms of anxiety (OR 5.04, 95% CI: 1.20–21.22, p = 0.008), female sex (OR 3.32, 95% CI: 1.02–10.76, p = 0.04) and longer disease duration (OR 1.13, 95% CI: 1.01–1.27, p = 0.04). Conclusions: Fatigue is highly prevalent even in patients with inactive IBD and is correlated with lower HR-QoL and anxiety, as well as with clinical factors such as longer disease duration and female sex.
Evaluation of Nutritional Status in an Acute Geriatric Unit: Retrospective Study and Analysis of Frailty Syndrome
Authors: Zulfiqar Abrar-Ahmad , Dembele Ibrahima Amadou , Andres Emmanuel
Published: March 2023

Introduction: The aim of our study is to evaluate the nutritional status of patients in an acute geriatric unit. Methods: Patients included in the study were hospitalized in an acute geriatric unit over a period of 6 months. The nutritional status of each patient was evaluated with anthropometric measurements (the BMI and MNA scales), and biological measurements (albumin). Frailty was evaluated using three scales: the Fried scale, the CFS and the modified SEGA scale. Results: A total of 359 patients were included, comprising 251 women (70%) with an average age of 85.28 years. The study showed that 102 elderly subjects were considered undernourished according to the BMI scale, 52 subjects were undernourished according to the MNA scale, and 50 subjects were undernourished according to their albumin levels. The relationships between undernutrition and frailty syndrome studied in our work show that elderly subjects who are undernourished according to the BMI and MNA scales are significantly frail according to Fried and Rockwood, whereas those who are undernourished according to their albumin levels are significantly frail according to Fried and the modified SEGA scale. Conclusion: The relationship between undernutrition and the frailty syndrome is close, and their joint screening is necessary, whether on an outpatient or in-hospital basis, in order to prevent negative events related to comorbidities and geriatric syndromes.
Health Technology Assessment of Different Glucosamine Formulations and Preparations Currently Marketed in Thailand
Authors: Bruyère Olivier , Detilleux Johann , Reginster Jean-Yves
Published: March 2023

Objective: The aim of this study was to evaluate the cost-effectiveness of different glucosamine formulations and preparations used for the management of osteoarthritis in Thailand compared with placebo. Methods: We used a validated model to simulate the individual patient Utility score from aggregated data available from 10 different clinical trials. We then used the Utility score to calculate the quality-adjusted life year (QALY) over 3 and 6 months treatment period. We used the public costs of glucosamine products available in Thailand in 2019 to calculate the incremental cost-effectiveness ratio. We separated the analyses for prescription-grade crystalline glucosamine sulfate (pCGS) and other formulations of glucosamine. A cost-effectiveness cut-off of 3.260 USD/QALY was considered. Results: Irrespective of the glucosamine preparation (tablet or powder/capsule), the data show that pCGS is cost-effective compared with placebo over a 3 and 6 months. However, the other glucosamine formulations (e.g., glucosamine hydrochloride) never reached the breakeven point at any time. Conclusions: Our data show that pCGS is cost-effective for the management of osteoarthritis in the Thai context while other glucosamine formulations are not.
Preparation of a Low-Protein-Fouling and High-Protein-Retention Membrane via Novel Pre-Hydrolysis Treatment of Polyacrylonitrile (PAN)
Authors: Xu Dong , Pan Guangyao , Ge Yutong , Yang Xuan
Published: March 2023

The attainment of high-protein-retention and low-protein-fouling membranes is crucial for industries that necessitate protein production or separation process. The present study aimed to develop a novel method for preparing polyacrylonitrile (PAN) membranes possessing a highly hydrophilic and negatively charged surface as well as interior structure. The method involved a pre-hydrolysis treatment during the preparation of the PAN dope solution, followed by phase inversion in an alkaline solution. Chemical and material characterization of the dopes and membranes uncovered that the cyclized PAN structure served as a reaction intermediate that facilitated strong hydrolysis effect during phase inversion and homogeneously formed carboxyl groups in the membrane’s interior structure. The resulting membrane showed a highly hydrophilic surface with a contact angle of 12.4° and demonstrated less than 21% flux decay and more than 95% flux recovery during multi-cycle filtration of bovine serum albumin (BSA) solution, with a high protein rejection rate of 96%. This study offers a facile and effective alternative for preparing PAN membranes with enhanced antifouling and protein-retention properties.
Metabolome × Microbiome Changes Associated with a Diet-Induced Reduction in Hepatic Fat among Adolescent Boys
Authors: Cohen Catherine C. , Huneault Helaina , Accardi Carolyn J. , Jones Dean P. , Liu Ken , Maner-Smith Kristal M. , Song Ming , Welsh Jean A. , Ugalde-Nicalo Patricia A. , Schwimmer Jeffrey B. , Vos Miriam B.
Published: March 2023

Dietary sugar reduction is one therapeutic strategy for improving nonalcoholic fatty liver disease (NAFLD), and the underlying mechanisms for this effect warrant further investigation. Here, we employed metabolomics and metagenomics to examine systemic biological adaptations associated with dietary sugar restriction and (subsequent) hepatic fat reductions in youth with NAFLD. Data/samples were from a randomized controlled trial in adolescent boys (11–16 years, mean ± SD: 13.0 ± 1.9 years) with biopsy-proven NAFLD who were either provided a low free-sugar diet (LFSD) (n = 20) or consumed their usual diet (n = 20) for 8 weeks. Plasma metabolomics was performed on samples from all 40 participants by coupling hydrophilic interaction liquid chromatography (HILIC) and C18 chromatography with mass spectrometry. In a sub-sample (n = 8 LFSD group and n = 10 usual diet group), 16S ribosomal RNA (rRNA) sequencing was performed on stool to examine changes in microbial composition/diversity. The diet treatment was associated with differential expression of 419 HILIC and 205 C18 metabolite features (p < 0.05), which were enriched in amino acid pathways, including methionine/cysteine and serine/glycine/alanine metabolism (p < 0.05), and lipid pathways, including omega-3 and linoleate metabolism (p < 0.05). Quantified metabolites that were differentially changed in the LFSD group, compared to usual diet group, and representative of these enriched metabolic pathways included increased serine (p = 0.001), glycine (p = 0.004), 2-aminobutyric acid (p = 0.012), and 3-hydroxybutyric acid (p = 0.005), and decreased linolenic acid (p = 0.006). Microbiome changes included an increase in richness at the phylum level and changes in a few genera within Firmicutes. In conclusion, the LFSD treatment, compared to usual diet, was associated with metabolome and microbiome changes that may reflect biological mechanisms linking dietary sugar restriction to a therapeutic decrease in hepatic fat. Studies are needed to validate our findings and test the utility of these “omics” changes as response biomarkers.
A Liquid Mirror Resonator
Authors: Haber Elad , Douvidzon Mark , Maayani Shai , Carmon Tal
Published: March 2023

We present the first experimental demonstration of a Fabry‒Perot resonator that utilizes total internal reflection from a liquid–gas interface. Our hybrid resonator hosts both optical and capillary waves that mutually interact. Except for the almost perfect reflection by the oil–air interface at incident angles smaller than the critical angle, reflections from the liquid-phase boundary permit optically examining thermal fluctuations and capillary waves at the oil surface. Characterizing our optocapillary Fabry‒Perot reveals optical modes with transverse cross-sectional areas of various shapes and longitudinal modes that are separated by the free spectral range. The optical finesse of our hybrid optocapillary resonator is Fo = 60, the optical quality factor is Qo = 20 million, and the capillary quality factor is Qc = 6. By adjusting the wavelength of our laser near the optical resonance wavelength, we measure the liquid’s Brownian fluctuations. As expected, the low-viscosity liquid exhibits a distinct frequency of capillary oscillation, indicating operation in the underdamped regime. Conversely, going to the overdamped regime reveals no such distinct capillary frequency. Our optocapillary resonator might impact fundamental studies and applications in surface science by enabling optical interrogation, excitation, and cooling of capillary waves residing in a plane. Moreover, our optocapillary Fabry‒Perot might permit photographing thermal capillary oscillation, which the current state-of-the-art techniques do not support.
Study on the Mechanism of Burr Formation by Simulation and Experiment in Ultrasonic Vibration-Assisted Micromilling
Authors: Zhang Yuanbin , Yuan Zhonghang , Fang Bin , Gao Liying , Chen Zhiyuan , Su Guosheng
Published: March 2023

Due to the strong plasticity of Inconel 718 and the significant size effect of micromachining, a large number of burrs will be produced in traditional processing. The addition of ultrasonic vibration during machining can reduce the burr problem. The mechanism of burr generation in traditional micromilling (TMM) and ultrasonic vibration-assisted micromilling (UVAMM) was analyzed by simulation, and verified by corresponding experiments. It is found that applying high-frequency ultrasonic vibration in the milling feed direction can reduce cutting temperature and cutting force, improve chip breaking ability, and reduce burr formation. When the cutting thickness will reach the minimum cutting thickness hmin, the chip will start to form. When A/ƒz > 1/2, the tracks of the two tool heads start to cut, and the chips are not continuous. Some of the best burr suppression effects were achieved under conditions of low cutting speed (Vc), feed per tooth (ƒz), and large amplitude (A). When A is 6 μm, the size and quantity of burr is the smallest. When ƒz reaches 6 μm, large continuous burrs appear at the top of the groove. The experimental results further confirm the accuracy of the simulation results and provide parameter reference.