bluuebunny/crossref_metadata_2025 huggingface dataset:
A Prospective, Cohort Study of SITOIGANAP to Treat Glioblastoma When Given in Combination With Granulocyte-Macrophage Colony-Stimulating Factor/Cyclophosphamide/Bevacizumab/Nivolumab or Granulocyte-Macrophage Colony-Stimulating Factor/Cyclophosphamide/Bevacizumab/Pembrolizumab in Patients Who Failed Prior Treatment With Surgical Resection, Radiation, and Temozolomide
Authors: Bota Daniela A. , Taylor Thomas H. , Lomeli Naomi , Kong Xiao-Tang , Fu Beverly D. , Schönthal Axel H. , Singer Samuel , Blumenthal Deborah T. , Senecal Frank M. , Linardou Helena , Rokas Evangelos , Antoniou Dimitris G. , Schijns Virgil E. J. C. , Chen Thomas C. , Elliot Joseph , Stathopoulos Apostolos
Published: June 2022

Background

Glioblastoma (GBM) is the most common primary, malignant brain tumor in adults and has a poor prognosis. The median progression-free survival (mPFS) of newly diagnosed GBM is approximately 6 months. The recurrence rate approaches 100%, and the case-fatality ratio approaches one. Half the patients die within 8 months of recurrence, and 5-year survival is less than 10%. Advances in treatment options are urgently needed. We report on the efficacy and safety of a therapeutic vaccine (SITOIGANAP: Epitopoietic Research Corporation) administered to 21 patients with recurrent GBM (rGBM) under a Right-to-Try/Expanded Access program. SITOIGANAP is composed of both autologous and allogeneic tumor cells and lysates.

Methods

Twenty-one patients with rGBM received SITOIGANAP on 28-day cycles in combination with granulocyte-macrophage colony-stimulating factor (GM-CSF), cyclophosphamide, bevacizumab, and an anti-programmed cell death protein-1 (anti-PD-1) monoclonal antibody (either nivolumab or pembrolizumab).

Results

The mPFS was 9.14 months, and the median overall survival (mOS) was 19.63 months from protocol entry. Currently, 14 patients (67%) are at least 6 months past their first SITOIGANAP cycle; 10 patients (48%) have received at least six cycles and have a mOS of 30.64 months and 1-year survival of 90%. The enrollment and end-of-study CD3+/CD4+ T-lymphocyte counts strongly correlate with OS.

Conclusions

The addition of SITOIGANAP/GM-CSF/cyclophosphamide to bevacizumab and an anti-PD-1 monoclonal antibody resulted in a significant survival benefit compared to historic control values in rGBM with minimal toxicity compared to current therapy.
Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B–Lamin B1 Interaction in Alcoholic Fatty Liver Disease
Authors: Qi Xiaoyu , Zheng Shuguo , Ma Mingyue , Lian Naqi , Wang Hongting , Chen Lerong , Song Anping , Lu Chunfeng , Zheng Shizhong , Jin Huanhuan
Published: June 2022

Recent studies indicated that hepatocyte senescence plays an important role in the development of alcoholic fatty liver disease (AFLD), suggesting that inhibition of hepatocyte senescence might be a potential strategy for AFLD treatment. The present study investigated the effect of curcumol, a component from the root of Rhizoma Curcumae, on hepatocyte senescence in AFLD and the underlying mechanisms implicated. The results showed that curcumol was able to reduce lipid deposition and injury in livers of ethanol liquid diet-fed mice and in ethanol-treated LO2 cells. Both in vivo and in vitro studies indicated that supplementation with curcumol effectively alleviated ethanol-induced cellular senescence as manifested by a decrease in senescence-associated β-galactosidase (SA-β-gal) activity, a downregulated expression of senescence-related markers p16 and p21, and dysfunction of the telomere and telomerase system. Consistently, treatment with curcumol led to a marked suppression of ethanol-induced formation of cytoplasmic chromatin fragments (CCF) and subsequent activation of cGAS-STING, resulting in a significant reduction in senescence-associated secretory phenotype (SASP)-related inflammatory factors’ secretion. Further studies indicated that curcumol’s inhibition of CCF formation might be derived from blocking the interaction of LC3B with lamin B1 and maintaining nuclear membrane integrity. Taken together, these results indicated that curcumol was capable of ameliorating AFLD through inhibition of hepatocyte senescence, which might be attributed to its blocking of LC3B and lamin B1 interaction and subsequent inactivation of the CCF-cGAS-STING pathway. These findings suggest a promising use of curcumol in the treatment of AFLD.
Effect of Exercise Training on Serum Transaminases in Patients With Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis
Authors: Hong Feng , Liu Yubo , Lebaka Veeranjaneya Reddy , Mohammed Arifullah , Ye Weibing , Chen Biqing , Korivi Mallikarjuna
Published: June 2022

Background/Purpose: Nonalcoholic fatty liver disease (NAFLD) constitutes a spectrum of liver diseases associated with various metabolic disorders. Exercise interventions reportedly manage the clinical outcomes of NAFLD, but their efficacy depends on exercise as well as characteristics of patient. We hypothesized that exercise could alleviate the elevated transaminases level, which may be associated with the characteristics of patients (age/bodyweight/sex) or exercise variables (frequency/intensity/duration). Therefore, we examined the effect of exercise on serum transaminases, and identified the variables influencing transaminases in NAFLD patients.

Methods: Article search was conducted using electronic databases (PubMed, Web of Science, EMBASE, ScienceDirect, Google Scholar) until December 2021. Studies that involved examination and comparison of the effect of an exercise intervention on alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in NAFLD/nonalcoholic steatohepatitis patients were included. We calculated pooled effect upon a meta-analysis, determined correlations (between transaminases and characteristics of patients/exercise) by meta-regression, and assessed the influencing variable through subgroup analysis.

Results: A total of 18 studies (22 trials) with 1098 NAFLD patients (exercise = 568; control = 530) were included. The pooled outcomes revealed that exercise intervention significantly decreased both ALT (p = 0.004) and AST (p = 0.001) levels in NAFLD patients. Meta-regression analysis showed decreased ALT (coef. = 1.138, p < 0.01) and AST (coef. = 0.459, p = 0.041) after intervention was correlated with the age of patients. Particularly, patients aged 30–39 years (MD: −25.89 U/L, 95% CI: −36.40 to −15.37, p < 0.00001) and 40–49 years (MD: −12.17 U/L, 95% CI: −20.38 to −3.96, p = 0.004) represented a substantial decrease in ALT levels. Additionally, the 50–59 years age group tended to have decreased ALT levels (MD: −3.94 U/L, 95% CI: −8.19 to 0.31, p = 0.07); however, patients above 60 years did not respond (p = 0.92) to exercise intervention. In contrast, exercise-induced AST reduction was found in only the 30–39 years age group (MD: −11.92 U/L, 95% CI: −16.78 to −7.06, p < 0.00001) and not in patients under the 40–49 (p = 0.19), and 50–59 groups (p = 0.12) and above 60 years (p = 0.15).

Conclusion: Our findings suggest that the age of NAFLD patients may be an important variable in improving the levels of serum transaminases, and clinically young patients may have greater benefits from exercise than older patients.
A Novel Combination of Genes Causing Temperature-Sensitive Hybrid Weakness in Rice
Authors: Than Kutay Soe  , Kunieda Mai , Sunohara Hidehiko , Inukai Yoshiaki , Reyes Vincent Pamugas , Nishiuchi Shunsaku , Doi Kazuyuki
Published: June 2022

Reproductive isolation is an obstacle for plant breeding when a distant cross is demanded. It can be divided into two main types based on different growth stages: prezygotic isolation and postzygotic isolation. The hybrid weakness, which is a type of postzygotic isolation, can become a problem in crop breeding. In order to overcome reproductive isolation, it is necessary to elucidate its mechanism. In this study, genetic analysis for low temperature-dependent hybrid weakness was conducted in a rice F2 population derived from Taichung 65 (T65, Japonica) and Lijiangxintuanheigu (LTH, Japonica). The weak and severe weak plants in F2 showed shorter culm length, late heading, reduced panicle number, decreased grain numbers per panicle, and impaired root development in the field. Our result also showed that hybrid weakness was affected by temperature. It was observed that 24°C enhanced hybrid weakness, whereas 34°C showed recovery from hybrid weakness. In terms of the morphology of embryos, no difference was observed. Therefore, hybrid weakness affects postembryonic development and is independent of embryogenesis. The genotypes of 126 F2 plants were determined through genotyping-by-sequencing and a linkage map consisting of 862 single nucleotide polymorphism markers was obtained. Two major quantitative trait loci (QTLs) were detected on chromosomes 1 [hybrid weakness j 1 (hwj1)] and 11 [hybrid weakness j 2 (hwj2)]. Further genotyping indicated that the hybrid weakness was due to an incompatible interaction between the T65 allele of hwj1 and the LTH allele of hwj2. A large F2 populations consisting of 5,722 plants were used for fine mapping of hwj1 and hwj2. The two loci, hwj1 and hwj2, were mapped in regions of 65-kb on chromosome 1 and 145-kb on chromosome 11, respectively. For hwj1, the 65-kb region contained 11 predicted genes, while in the hwj2 region, 22 predicted genes were identified, two of which are disease resistance-related genes. The identified genes along these regions serve as preliminary information on the molecular networks associated with hybrid weakness in rice.
Joint Transcriptomic and Metabolomic Analysis Reveals Differential Flavonoid Biosynthesis in a High-Flavonoid Strawberry Mutant
Authors: Lin Yuanxiu , Hou Guoyan , Jiang Yuyan , Liu Xiaoyang , Yang Min , Wang Liangxin , Long Yu , Li Mengyao , Zhang Yunting , Wang Yan , Chen Qing , Zhang Yong , Wang Xiaorong , Tang Haoru , Luo Ya
Published: June 2022

The enriched phenolic content attributes to the promising health benefit of strawberry fruits. On behalf of screening and seeking the breeding material with high phytochemical composition, a mutant (MT) of strawberry ‘Benihoppe’ (WT) with high total flavonoid content (TFC), especially anthocyanins and proanthocyanidins (PAs), was identified in this study. To investigate the possible reason for these disparities during strawberry fruit development, an integrated transcriptomic and metabolomic analysis was conducted using these two specific materials. As a result, a total of 113 flavonoid compounds were detected, a specific anthocyanin, namely, petunidin 3-O-rutinoside was detected for the first time in strawberry. By comparing with the WT fruits, a significant reduction of petunidin 3-O-rutinoside while around 24 times higher of cyanidin 3-O-rutinoside in MT fruits were observed. However, the cyanidin 3-glucoside content did not show obvious changes between MT and WT fruits, the pelargonidin and its derivatives were up-regulated only in partial red (PR) stage, but not in large green (LG) and fully red (FR) stages. Notably, the PAs such as procyanidin B2, procyanidin A1, catechin, gallocatechin gallate, epigallacatechin, and theaflavin were markedly up-regulated in MT. These results revealed a differential flavonoid biosynthesis between the two detected strawberry genotypes. A joint analysis with transcriptome data explained the up-regulation of cyanidin-based anthocyanins and PAs were caused by the down-regulation of F3′5′H, and up-regulation of F3'H and LAR expression, which might be regulated by the upregulation of potential TFs such as C3H, MADS, and AP2/ERF TFs. Metabolite correlation analysis suggested that it was PAs but not anthocyanins strongly correlated with the total phenolic content (TPC), indicated that PAs might contribute more to TPC than anthocyanins in our detected strawberry samples. This study not only potentially provided a new mutant for further breeding program to obtain high flavonoid content strawberry but also gave insights into strawberry flavonoid metabolic regulatory network, laid the foundation for identifying new flavonoid regulators in strawberry.
Differing Responses to Phytophthora cinnamomi Infection in Susceptible and Partially Resistant Persea americana (Mill.) Rootstocks: A Case for the Role of Receptor-Like Kinases and Apoplastic Proteases
Authors: Backer Robert , Engelbrecht Juanita , van den Berg Noëlani
Published: June 2022

The hemibiotrophic plant pathogen Phytophthora cinnamomi Rands is the most devastating pathogen of avocado (Persea americana Mill.) and, as such, causes significant annual losses in the industry. Although the molecular basis of P. cinnamomi resistance in avocado and P. cinnamomi virulence determinants have been the subject of recent research, none have yet attempted to compare the transcriptomic responses of both pathogen and host during their interaction. In the current study, the transcriptomes of both avocado and P. cinnamomi were explored by dual RNA sequencing. The basis for partial resistance was sought by the inclusion of both susceptible (R0.12) and partially resistant (Dusa®) rootstocks sampled at early (6, 12 and 24 hours post-inoculation, hpi) and late time-points (120 hpi). Substantial differences were noted in the number of differentially expressed genes found in Dusa® and R0.12, specifically at 12 and 24 hpi. Here, the partially resistant rootstock perpetuated defense responses initiated at 6 hpi, while the susceptible rootstock abruptly reversed course. Instead, gene ontology enrichment confirmed that R0.12 activated pathways related to growth and development, essentially rendering its response at 12 and 24 hpi no different from that of the mock-inoculated controls. As expected, several classes of P. cinnamomi effector genes were differentially expressed in both Dusa® and R0.12. However, their expression differed between rootstocks, indicating that P. cinnamomi might alter the expression of its effector arsenal based on the rootstock. Based on some of the observed differences, several P. cinnamomi effectors were highlighted as potential candidates for further research. Similarly, the receptor-like kinase (RLK) and apoplastic protease coding genes in avocado were investigated, focusing on their potential role in differing rootstock responses. This study suggests that the basis of partial resistance in Dusa® is predicated on its ability to respond appropriately during the early stages following P. cinnamomi inoculation, and that important components of the first line of inducible defense, apoplastic proteases and RLKs, are likely to be important to the observed outcome.
The Influence of Science Technology Engineering Arts Mathematics-Based Psychological Capital Combined With Ideological and Political Education on the Entrepreneurial Performance and Sports Morality of College Teachers and Students
Authors: Jin Ying
Published: June 2022

This study aims to alleviate the current tense employment situation and study the entrepreneurial situation of teachers and students in colleges and universities. Firstly, based on the educational concept of Science Technology Engineering Arts Mathematics (STEAM), Ideological and Political Education (IPE) is added to psychological capital to explore the effect of the combination of the two on entrepreneurial performance. An entrepreneurial performance impact model is constructed, and the questionnaire is set. Secondly, the influence of psychological capital combined with IPE on sports morality is explored, and a sports morality questionnaire is designed. Finally, the questionnaire results are analyzed. The results showed that most of the participants in the survey are under the age of 25. The 25- to 29-year-olds are the smallest among those surveyed. The respondents who participated in the survey had the most undergraduate degrees, accounting for 43.4%. The 40 items on entrepreneurial performance this time obey a normal distribution, and the scale has good reliability and validity. The main factor analysis results obtained by principal component analysis include 6 factors. Their total explanatory power exceeds 67%, and the six factors screened out this time are well represented. The model tolerance is between 0.45 and 0.5, and the Variance Inflation Factor (VIF) value is less than 10. The scale does not suffer from multi-collinearity issues. IPE significantly strengthens the effect of psychological capital on entrepreneurial performance. The influences of various sports on sportsmanship, bravery, self-confidence, and self-transcendence are significantly different, and the P value is less than 0.001. The difference in aggressiveness is less than 0.01, indicating that it is very significant. Different sports have significant differences in the cultivation of sports morality, and the P value is less than 0.05. The differences in self-discipline are also significant, and there are extremely significant differences in compliance with rules, and the P value here is less than 0.001. There are no significant differences in sporting qualities. IPE combined with psychological capital has a significant impact on entrepreneurial performance. These contents provide references for the entrepreneurship education of teachers and students in colleges and universities. The contribution lies in expanding the research on psychological capital in entrepreneurial performance and laying a foundation for the combination of psychological capital and IPE.
ICT Self-Efficacy, Organizational Support, Attitudes, and the Use of Blended Learning: An Exploratory Study Based on English Teachers in Basic Education
Authors: Ye Long , Kuang Manteng , Liu Song
Published: June 2022

The study aims to build a model that predicts the behavior of the use of blended learning by English teachers of basic education in China in the environment of repeated lockdowns during the COVID-19 pandemic. It examines the relationships between ICT self-efficacy, organizational support for blended learning, attitudes toward blended learning, and the use of blended learning. Data were collected from 562 teachers using a survey questionnaire. Employing partial least squares structural equation modeling (PLS-SEM), a hypothesized model was tested for path coefficients and predictive power. This study found that ICT self-efficacy and organizational support for blended learning are sound predictors of teachers' use of blended learning and that the former appeared to be a stronger determinant. In addition, attitudes toward blended learning can directly influence teachers' use of blended learning and mediate the relationship between ICT efficacy and the use of blended learning and between organizational support for blended learning and the use of blended learning. These three variables account for 54.7% of the variance in teachers' use of blended learning.
Anticipated Self and Public Stigma in Suicide Prevention Professionals
Authors: Roškar Saška , Kralj Domen , Andriessen Karl , Krysinska Karolina , Vinko Matej , Podlesek Anja
Published: June 2022

Background

Stigma about mental illness—both public and self—is one of the most important factors hindering help-seeking. Stigma can occur during an acute episode of mental illness or be anticipatory. One group affected by stigma, but often neglected, is mental health professionals. This study examined the anticipated form of mental-illness and help-seeking self-stigma and the anticipated form of public stigma of suicidal behavior among members of the International Association for Suicide Prevention. We hypothesized that suicidologists with a history of suicidality or mental illness would anticipate greater stigma from the public and self.

Methods

The study received ethical approval from the Commission for Medical Ethics of the Republic of Slovenia. Data from 83 participants who completed an online survey (February to May 2020) with informed consent were analyzed using path analysis. We tested a model predicting help-seeking self-stigma based on (i) personal experience of mental illness using anticipated self-stigma of mental illness as a mediating variable and (ii) history of suicidal behavior using anticipated public stigma of suicidal behavior as a mediating variable.

Results

Personal experience of mental illness predicted anticipation of self-stigma of mental illness (β = 0.26). History of suicidality predicted anticipation of public stigma of suicidal behavior (β = 0.29). Anticipated self-stigma of mental illness proved to be a stronger predictor of help-seeking self-stigma (β = 0.40) than anticipated public stigma of suicidal behavior (β = 0.07).

Conclusions

It is important to intentionally support the mental health of suicide prevention professionals, as they are not immune to mental illness or various types of stigma. Because our sample was small and diverse, further research to better understand stigma concepts in this population is warranted.
Analysis of Spatio-Temporal Characteristics of Urban Economic Resilience and Influencing Factors in Guangdong-Hong Kong-Macao Greater Bay Area
Authors: Zhang Yuanyuan , Chen Zehui , Tang Bo , Sun Hua
Published: June 2022

Due to the changes in the domestic and international economic situation in the post-pandemic era, the economic development of the Guangdong-Hong Kong-Macao Greater Bay Area has become unstable in many aspects. The paper adopted the Pressure-State-Response (PSR) model to build a regional economic resilience evaluation system from the perspective of public health emergencies. Then, the spatial and temporal evolution of the economic resilience of the Guangdong-Hong Kong-Macao Greater Bay Area and the influencing factors were explored by using entropy weight method, GIS and gray correlation method. The conclusions show that: (1) Temporally, the economic resilience of the Guangdong-Hong Kong-Macao Greater Bay Area has generally increased from 2010 to 2021, and is divided into three main stages: rapid development, adjustment to fluctuations and stable development. (2) Spatially, the overall pattern of economic resilience in the Guangdong-Hong Kong-Macao Greater Bay Area is high in the middle and south and low in the northwest, and shows a “stochastic—equalized—polarized” pattern of transformation. (3) In terms of influencing factors, economic status and economic response are the main dimensions affecting the resilience level of the economic system in the Guangdong-Hong Kong-Macao region. The level of scientific research and innovation, medical governance, government regulation and the rationalization of the industrial system are the key factors.
Federated Learning for Privacy-Aware Human Mobility Modeling
Authors: Ezequiel Castro Elizondo Jose , Gjoreski Martin , Langheinrich Marc
Published: June 2022

Human mobility modeling is a complex yet essential subject of study related to modeling important spatiotemporal events, including traffic, disease spreading, and customized directions and recommendations. While spatiotemporal data can be collected easily via smartphones, current state-of-the-art deep learning methods require vast amounts of such privacy-sensitive data to generate useful models. This work investigates the creation of spatiotemporal models using a Federated Learning (FL) approach—a machine learning technique that avoids sharing personal data with centralized servers. More specifically, we examine three centralized models for next-place prediction: a simple Gated Recurrent Unit (GRU) model, as well as two state-of-the-art centralized approaches, Flashback and DeepMove. Flashback is a Recurrent Neural Network (RNN) that utilizes historical hidden states with similar context as the current spatiotemporal context to improve performance. DeepMove is an attentional RNN that aims to capture human mobility's regularity while coping with data sparsity. We then implemented models based on FL for the two best-performing centralized models. We compared the performance of all models using two large public datasets: Foursquare (9,450 million check-ins, February 2009 to October 2010) and Gowalla (3,300 million check-ins, April 2012 to January 2014). We first replicated the performance of both Flashback and DeepMove, as reported in the original studies, and compared them to the simple GRU model. Flashback and GRU proved to be the best performing centralized models, so we further explored both in FL scenarios, including several parameters such as the number of clients, rounds, and epochs. Our results indicated that the training process of the federated models was less stable, i.e., the FL versions of both Flashback and GRU tended to have higher variability in the loss curves. The higher variability led to a slower convergence and thus a poorer performance when compared to the corresponding centralized models. Model performance was also highly influenced by the number of federated clients and the sparsity of the evaluation dataset. We additionally provide insights into the technical challenges of applying FL to state-of-the-art deep learning methods for human mobility.
Engaging Scientific Diasporas in STEAM Education: The Case of Science Clubs Colombia
Authors: Avendano-Uribe Bryann E. , Lombana-Bermudez Andres , Flórez Laura V. , Chaparro Elisa , Hernandez-Morales Adriana Carolina , Archbold Jorge , Buitrago-Casas Juan Camilo , Porras Ana Maria
Published: June 2022

Currently, there is limited insight on the role that scientific diasporas can play in STEAM education in Latin America. Here, we present the Science Clubs Colombia (Clubes de Ciencia Colombia-SCC) program, a pioneering STEAM capacity-building initiative led by volunteer scientists to engage youth and children from underserved communities in science. The program brings together researchers based in Colombia and abroad to lead intensive project-based learning workshops for young students in urban and rural areas. These projects focus on channeling the students' technical and cognitive scientific aptitudes to tackle challenges of both local and global relevance. The program provides high-quality STEAM education adapted to communities' needs and articulates long-lasting international collaborations using the mobility of the Colombian diaspora. The program's success is tangible via its sustained growth and adaptability. Since its first version in 2015, 722 volunteer scientists living abroad or in Colombia have collaborated to create 364 clubs with the participation of 9,295 students. We describe elements of the SCC program that lead to a scalable and reproducible outcome to engage science diasporas in STEAM education. Additionally, we discuss the involvement of multiple stakeholders and the generation of international networks as potential science diplomacy outcomes. The SCC program strengthens the involvement of Latin American youth in science, demonstrates the potential of engaging scientific diasporas in science education, and enriches connections between the Global South and the Global North.
Water-Saving Tips With a Visualized Indicator Related to the Environment
Authors: Otaki Yurina , Maeda Asahi
Published: June 2022

Water-saving tips are information-based interventions aimed at managing water demand. Studies of the effectiveness of qualitative water-saving tips to explain why and how water should be saved have not always found significant changes in water consumption. Some studies indicate that the addition of quantitative tips and videos are effective for water conservation. This study adds to the literature by attempting to verify the effectiveness of water-saving tips that visualize the amount of water that can be saved using illustrations of bathtubs. Furthermore, as not all people are interested in water saving, we include tips with a visualized indicator related to the environment, which is of more general interest. These tips used CO2 emissions reductions to demonstrate the effect of water saving. As a result, for high-consumption households, it is more effective to present water-saving tips that present information on how their actions can limit CO2 emissions than to illustrate the reduction in water consumption directly. At the same time, for low-consumption households, none of the tips had neither water-saving nor boomerang effects (i.e., increased the consumption while planning to reduce). Households were divided into high-and low-consumption using baseline water consumption per capita for their household size. The results of this study could serve as a tool for water demand management that can be easily utilized in many parts of the world.
Access to and Utilization of Wild Species for Food and Nutrition Security in Teso and Acholi Sub-regions of Uganda
Authors: Ekesa Beatrice , Fongar Andrea , Nasser Mulumba
Published: June 2022

Wild foods significantly contribute to the global food basket, and food and nutrition security. Worldwide, wild food species form an integral part of local diets and their widespread assimilation into local food culture suggests an untapped potential to ensure easy availability and access to micronutrients for sustainable food systems. However, wild species are often overlooked within nutrition-related policies, and their levels of availability remain unknown. This paper, therefore, focuses on understanding the changes in availability, access and utilization of wild animal and plant species in Teso and Acholi sub-regions of Uganda. A four-cell agrobiodiversity mapping protocol was applied through focus group discussions to establish the different wild animal and plant species that have played and still play a role within local communities' livelihoods in the two sub-regions. Findings showed that at the time of the study (2017), wild foods were considered to be important contributors to food and nutrition security, although the number of species reported to be available was slightly lower [91 (Acholi) and 103 (Teso)], compared to 20 years ago, where around 109 edible wild species were reportedly available in both the Acholi and Teso sub-regions. Reasons for the decline included (i) increased cultivations, and (ii) natural habitat destruction due to settlements and changes in land ownership. In the latter case, individual owners have further fragmented their land and do not allow villagers to freely participate in hunting and gathering. Lastly, rebel unrest increased feelings of insecurity, and thus prompted a decline in hunting and gathering. The noted presence and contribution of wild foods in Teso and Acholi calls for collective efforts to increase access to knowledge on the value of these wild foods for not only food and nutrition but also for their potential contribution to the social and cultural lives of the people.
Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep
Authors: Wu Xinmiao , Zhen Huimin , Liu Yan , Li Lu , Luo Yuzhu , Liu Xiu , Li Shaobin , Hao Zhiyun , Li Mingna , Hu Liyan , Qiao Lirong , Wang Jiqing
Published: June 2022

Circular RNAs (circRNAs) are a kind of non-coding RNA that have an important molecular function in mammary gland development and lactation of mammals. In our previous study, circ_015343 was found to be highly expressed in the ovine mammary gland tissue at the peak-lactation period by using RNA sequencing (RNA-seq). In the present study, the authenticity of circ_015343 was confirmed by using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and Sanger sequencing. The circ_015343 was derived from the complete 10 exons of aminoadipic semialdehyde synthase (AASS), ranging from exon 2 to exon 11 and mainly located in cytoplasm of ovine mammary epithelial cells. The circRNA was found to be expressed in eight ovine tissues, with the highest expression level in the mammary gland and the least expression in Longissimus dorsi muscle. The circ_015343 had a lower level of expression in a sheep breed with higher milk yield and milk fat content. The disturbed circ_015343 increased the viability and proliferation of the ovine mammary epithelial cells. The inhibition of circ_015343 also increased the expression levels of three milk fat synthesis marker genes: acetyl-coenzyme A carboxylase alpha (ACACA), fatty acid-binding protein 4 (FABP4), and sterol regulatory element-binding protein 1 (SREBP1), as well as three proliferation-related genes: cyclin dependent kinase 2 (CDK2), cyclin dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA), but decreased the expression level of its parent gene AASS. A circRNA-miRNA-mRNA interaction network showed that circ_015343 would bind some microRNAs (miRNAs) to regulate the expression of functional genes related to the development of mammary gland and lactation. This study contributes to a better understanding of the roles of circ_015343 in the mammary gland of sheep.
Integrated Development of a Topology-Optimized Compliant Mechanism for Precise Positioning
Authors: Hu Yaoyuan , Ju Bingfeng , Zhu Wule
Published: June 2022

A scheme for modelling and controlling a two-dimensional positioning system with a topology-optimized compliant mechanism is presented. The system is designed to ensure a relatively large workspace and exhibit robustness against system nonlinearities. A detailed design procedure based on topology optimization is presented, and a nonlinear description of the designed mechanism is developed as a starting point for further precise position control. The theoretical model is shown to be suitable for a considerably larger working range without losing consistency. A backstepping controller is employed to manipulate the nonlinearities in the model resulting from the geometrical and material nonlinearity of the mechanical structure. The hysteresis of the piezoelectric actuator is also taken into consideration. An experimental verification of the controller demonstrates that the proposed design approach improves the performance of compliant mechanism and satisfies the needs for precision positioning.
Study on the Method of Matched Splice Grafting for Melon Seedlings Based on Visual Image
Authors: Xu Pengyun , Zhang Tong , Chen Liping , Huang Wenqian , Jiang Kai
Published: June 2022

Due to the cutting mechanism of the existing grafting machine, it cannot adjust the cutting angle in real time, resulting in low fitting precision on the cutting surfaces between the rootstocks and scion seedlings and, thus, seriously affecting the survival rate and quality of the grafting seedlings. In this paper, a kind of splice grafting method based on visual image is proposed, aiming at maximizing the joint rate between cutting surfaces of rootstocks and scion seedlings and realizing precise cutting and grafting of grafting machine. After analysis, we determined that melon rootstock seedlings have a structure of pith cavity inside, and the solid structure from the top of the pith cavity to the left and right base points of a growing point forms the important area of a cutting surface. In order to obtain the geometric model of the cutting surfaces of the seedlings, a visual image analysis system was established to identify, analyze, and model the pith cavity structure inside the rootstock seedling, as well as the external morphological characteristics, and the ultimate cutting angle of the rootstock seedling and cutting surface parameters were determined. By measuring the length of minor axis of scion seedlings in order to achieve the maximum joint rate, the optimal cutting angle of the rootstocks and scion seedlings was determined. Then grafting and seedling cultivation tests were carried out. The test results showed that the range of ultimate cutting angle on rootstock seedlings (Cucurbita moschata) was 18.21 ± 1.92°; the cutting angles of the rootstock (Cucurbita moschata) and scion seedlings (watermelon) were 22° and 19.68°, respectively; the cutting surface length of the two was 4.96 mm; and the cutting surface thickness of the rootstock was 0.13 mm, all of which could satisfy the technological requirements of the matched splice grafting of melons. The research results can serve as a reference for the design in vision-guided precision cutting and real-time grafting operation on grafting robots.
Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato
Authors: Khapte Pratapsingh S. , Kumar Pradeep , Wakchaure Goraksha C. , Jangid Krishna Kumar , Colla Giuseppe , Cardarelli Mariateresa , Rane Jagadish
Published: June 2022

The cultivation of nutritionally and economically important crops like tomato are often threatened by dry spells due to drought as these crops largely depend on an assured water supply. The magnitude and intensity of drought is predicted to intensify under climate change scenarios, particularly in semi-arid regions, where water is already a scarce resource. Hence, it is imperative to devise strategies to mitigate the adverse effects of drought on tomato through improvement in the plant’s efficiency to utilise the moisture in the growth medium. Since the root is the entry point for water, its intrinsic structure and functions play a crucial role in maintaining the soil–water–plant continuum during moisture deficit at the rhizosphere. Grafting offers a great opportunity to replace the root system of the cultivated tomato plants with that of wild species and hence provide a rapid solution to modulate root system architecture in contrast to the time-consuming conventional breeding approach. However, the success in developing the best graft combination of cultivated tomato and rootstock depends on the source of rootstock and selection methods. In this study, we used a high throughput phenomics facility to assess the efficiency of tomato, grafted on the rootstocks of different genetic backgrounds, at different levels of moisture in the soil. Rootstocks included tomato cultivars and the hybrids, derived from the crosses involving wild relatives, as donor parents. Among the rootstocks, an interspecific (Solanum lycopersicum × S. pennellii) derivative RF4A was highly efficient in terms of productive use of water. The RF4A rootstock-grafted plants were more conservative in water use with higher plant water status through relatively better stomatal regulation and hence were more efficient in generating greater biomass under water stress conditions. These plants could maintain a higher level of PSII efficiency, signifying better photosynthetic efficiency even under water stress. The distinct response of interspecific rootstock, RF4A, to water stress can be ascribed to the effective root system acquired from a wild parent (S. pennellii), and hence efficient water uptake. Overall, we demonstrated the efficient use of a phenomics platform and developed a protocol to identify promising rootstock–scion combinations of tomato for optimization of water use.
Effects of Supplementing Finishing Goats with Mitragyna speciosa (Korth) Havil Leaves Powder on Growth Performance, Hematological Parameters, Carcass Composition, and Meat Quality
Authors: Chanjula Pin , Wungsintaweekul Juraithip , Chiarawipa Rawee , Phesatcha Kampanat , Suntara Chanon , Prachumchai Rittikeard , Pakdeechanuan Patcharin , Cherdthong Anusorn
Published: June 2022

The objective of this study was to see how dried Mitragyna speciosa Korth leaves (DKTL) affected growth, hematological parameters, carcass characteristics, muscle chemical composition, and fatty acid profile in finishing goats. In a randomized complete block design, twenty crossbred males (Thai Native x Boer) weaned goats (17.70 ± 2.50 kg of initial body weight (BW)) were provided to the experimental animals (5 goats per treatment) for 90 days. Individual dietary treatments of 0, 2.22, 4.44, and 6.66 g/d of DKTL on a dry matter basis were given to the goats. The diets were provided twice daily as total mixed rations ad libitum. In comparison to the control diet, DKTL supplementation had no effect on BW, average daily gain (ADG), feed conversion ratio (FCR), carcass composition, meat pH, or meat color (p > 0.05). After DKTL treatment, the hot carcass weight, longissimus muscle area, oleic acid (C18:1n9), monounsaturated fatty acid (MUFA), and protein content increased, but saturated fatty acids (SFA) and ether extract decreased (p < 0.05). To summarize, DKTL supplementation can improve goat meat quality.
Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen
Authors: Del Prete Chiara , Calabria Alfonso , Longobardi Valentina , Palumbo Veronica , Merlo Barbara , Iacono Eleonora , Tafuri Simona , Carotenuto Domenico , Ciani Francesca , Damiano Sara , Ciarcia Roberto , Cocchia Natascia
Published: June 2022

Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs were cooled for 7 days in the absence (control group) or in the presence of 10, 20 and 50 μL/mL of an aqueous extract of Maca. Sperm were evaluated for sperm viability, motility, DNA fragmentation and lipid peroxidation after 3 h, 24 h, 4 days and 7 days of storage. The addition of 10 μL/mL of Maca preserved sperm DNA and plasma membrane integrity at 3 h and increased sperm curvilinear velocity after 24 h. Treatment with 20 and 50 μL/mL of Maca increased the percentage of hyperactivated sperm after 3 h. Moreover, semen treated with 20 μL/mL of Maca decreased lipid peroxidation at 24 h. A significant reduction of sperm DNA and plasma membrane integrity as well as of kinetics parameters between 3 and 24 h of refrigerated storage with the higher concentration tested was observed. Although Maca was not able to protect canine semen with extended refrigeration storage time, it increased hyperactivation and preserved DNA integrity in short-term storage.
Oral Palatability Testing of a Medium-Chain Triglyceride Oil Supplement (MCT) in a Cohort of Healthy Dogs in a Non-Clinical Setting
Authors: Berk Benjamin Andreas , Packer Rowena Mary-Anne , Fritz Julia , Volk Holger Andreas
Published: June 2022

The oral palatability of functional foods such as medium-chain triglycerides (MCT) play a crucial role in owner and patient compliance when used as an adjunct in the management of health conditions such as epilepsy. Despite the promising benefits, the palatability of MCT has not undergone a more recent evaluation in dogs. The aim of this study was to assess the palatability and tolerance of short-term, daily supplementation of a 10% metabolic energy based MCT oil volume compared to a tasteless control oil in healthy dogs. An at-home, randomized, double-blinded, controlled single-bowl palatability test with three five-days phases was conducted. Data were collected from nineteen healthy dogs via study visits, feeding diary and eating questionnaires. No difference in the average food intake or intake ratio between food with and without oil supplementation or between the two oil groups was found. The mean food intake time was longer under MCT. In conclusion, MCT oil given as a short-term supplement is well tolerated and palatable in a healthy canine population, with only some changes in eating behaviour. Our results support earlier evidence that MCT oil is a well-tolerated additive in the nutritional management of different diseases such as epilepsy or dementia in dogs.
Oilseed Supplementation Improves Milk Composition and Fatty Acid Profile of Cow Milk: A Meta-Analysis and Meta-Regression
Authors: Plata-Pérez Genaro , Angeles-Hernandez Juan C. , Morales-Almaráz Ernesto , Del Razo-Rodríguez Oscar E. , López-González Felipe , Peláez-Acero Armando , Campos-Montiel Rafael G. , Vargas-Bello-Pérez Einar , Vieyra-Alberto Rodolfo
Published: June 2022

Oilseed supplementation is a strategy to improve milk production and milk composition in dairy cows; however, the response to this approach is inconsistent. Thus, the aim of this study was to evaluate the effect of oilseed supplementation on milk production and milk composition in dairy cows via a meta-analysis and meta-regression. A comprehensive and structured search was performed using the following electronic databases: Google Scholar, Primo-UAEH and PubMed. The response variables were: milk yield (MY), atherogenic index (AI), Σ omega-3 PUFA, Σ omega-6 PUFA, fat, protein, lactose, linoleic acid (LA), linolenic acid (LNA), oleic acid (OA), vaccenic acid (VA), conjugated linoleic acid (CLA), unsaturated fatty acid (UFA) and saturated fatty acid (SFA) contents. The explanatory variables were breed, lactation stage (first, second, and third), oilseed type (linseed, soybean, rapeseed, cottonseed, and sunflower), way (whole, extruded, ground, and roasted), dietary inclusion level, difference of the LA, LNA, OA, forage and NDF of supplemented and control rations, washout period and experimental design. A meta-analysis was performed with the “meta” package of the statistical program R. A meta-regression analysis was applied to explore the sources of heretogeneity. The inclusion of oilseeds in dairy cow rations had a positive effect on CLA (+0.27 g 100 g−1 fatty acids (FA); p < 0.0001), VA (+1.03 g 100 g−1 FA; p < 0.0001), OA (+3.44 g 100 g−1 FA; p < 0.0001), LNA (+0.28 g 100 g−1 FA; p < 0.0001) and UFA (+8.32 g 100 g−1 FA; p < 0.0001), and negative effects on AI (−1.01; p < 0.0001), SFA (−6.51; p < 0.0001), fat milk (−0.11%; p < 0.001) and protein milk (−0.04%; p < 0.007). Fat content was affected by animal breed, lactation stage, type and processing of oilseed and dietary NDF and LA contents. CLA, LA, OA and UFA, desirable FA milk components, were affected by type, processing, and the intake of oilseed; additionally, the concentrations of CLA and VA are affected by washout and design. Oilseed supplementation in dairy cow rations has a positive effect on desirable milk components for human consumption. However, animal response to oilseed supplementation depends on explanatory variables related to experimental design, animal characteristics and the type of oilseed.
Thiamine Demonstrates Bio-Preservative and Anti-Microbial Effects in Minced Beef Meat Storage and Lipopolysaccharide (LPS)-Stimulated RAW 264.7 Macrophages
Authors: Hsouna Anis Ben , Boye Alex , Ackacha Bouthaina Ben , Dhifi Wissal , Saad Rania Ben , Brini Faiçal , Mnif Wissem , Kačániová Miroslava
Published: June 2022

This study assessed the anti-inflammatory effect of thiamine (TA) in lipopolysaccharide-stimulated RAW264.7 cells and also assessed the preservative properties of TA in minced beef. TA demonstrated a concentration-dependent antimicrobial effect on microbial contaminants. Inhibition zones and MIC from the effect of TA on the tested bacterial strains were respectively within the ranges 15–20 mm and 62.5–700 µg/mL. TA significantly (p < 0.05) decreased all the pro-inflammatory factors [(nitric oxide (NO), prostaglandin E2 (PGE2), TNF-α, IL-6, IL-1β, and nuclear factor-κB (NF-κB)] monitored relative to LPS-stimulated RAW264.7 cells. TA inhibited the expression of both iNOS and COX-2. In minced beef flesh, the growth of Listeria monocytogenes was inhibited by TA. TA improved physicochemical and microbiological parameters of stored minced beef meat compared to control. Principal component analyses and heat maps elucidate the quality of the tested meats.
The Promise of Behavioral Tracking Systems for Advancing Primate Animal Welfare
Authors: Knaebe Brenna , Weiss Claudia C. , Zimmermann Jan , Hayden Benjamin Y.
Published: June 2022

Recent years have witnessed major advances in the ability of computerized systems to track the positions of animals as they move through large and unconstrained environments. These systems have so far been a great boon in the fields of primatology, psychology, neuroscience, and biomedicine. Here, we discuss the promise of these technologies for animal welfare. Their potential benefits include identifying and reducing pain, suffering, and distress in captive populations, improving laboratory animal welfare within the context of the three Rs of animal research (reduction, refinement, and replacement), and applying our understanding of animal behavior to increase the “natural” behaviors in captive and wild populations facing human impact challenges. We note that these benefits are often incidental to the designed purpose of these tracking systems, a reflection of the fact that animal welfare is not inimical to research progress, but instead, that the aligned interests between basic research and welfare hold great promise for improvements to animal well-being.