bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization and Prediction of the Drying and Quality of Turnip Slices by Convective-Infrared Dryer under Various Pretreatments by RSM and ANFIS Methods
Authors: Taghinezhad Ebrahim , Kaveh Mohammad , Szumny Antoni
Published: January 2021

Drying can prolong the shelf life of a product by reducing microbial activities while facilitating its transportation and storage by decreasing the product weight and volume. The quality factors of the drying process are among the important issues in the drying of food and agricultural products. In this study, the effects of several independent variables such as the temperature of the drying air (50, 60, and 70 °C) and the thickness of the samples (2, 4, and 6 mm) were studied on the response variables including the quality indices (color difference and shrinkage) and drying factors (drying time, effective moisture diffusivity coefficient, specific energy consumption (SEC), energy efficiency and dryer efficiency) of the turnip slices dried by a hybrid convective-infrared (HCIR) dryer. Before drying, the samples were treated by three pretreatments: microwave (360 W for 2.5 min), ultrasonic (at 30 °C for 10 min) and blanching (at 90 °C for 2 min). The statistical analyses of the data and optimization of the drying process were achieved by the response surface method (RSM) and the response variables were predicted by the adaptive neuro-fuzzy inference system (ANFIS) model. The results indicated that an increase in the dryer temperature and a decline in the thickness of the sample can enhance the evaporation rate of the samples which will decrease the drying time (40–20 min), SEC (from 168.98 to 21.57 MJ/kg), color difference (from 50.59 to 15.38) and shrinkage (from 67.84% to 24.28%) while increasing the effective moisture diffusivity coefficient (from 1.007 × 10−9 to 8.11 × 10−9 m2/s), energy efficiency (from 0.89% to 15.23%) and dryer efficiency (from 2.11% to 21.2%). Compared to ultrasonic and blanching, microwave pretreatment increased the energy and drying efficiency; while the variations in the color and shrinkage were the lowest in the ultrasonic pretreatment. The optimal condition involved the temperature of 70 °C and sample thickness of 2 mm with the desirability above 0.89. The ANFIS model also managed to predict the response variables with R2 > 0.96.
Influence of Bacterial Competitors on Salmonella enterica and Enterohemorrhagic Escherichia coli Growth in Microbiological Media and Attachment to Vegetable Seeds
Authors: Liu Da , Walcott Ronald , Mis Solval Kevin , Chen Jinru
Published: January 2021

Interests in using biological agents for control of human pathogens on vegetable seeds are rising. This study evaluated whether probiotic bacterium Lactobacillus rhamnosus GG, bacterial strains previously used as biocontrol agents in plant science, as well as a selected plant pathogen could compete with foodborne human pathogens, such as Salmonella enterica and enterohemorrhagic Escherichia coli (EHEC), for growth in microbiological media and attachment to vegetable seeds; and to determine whether the metabolites in cell-free supernatants of competitive bacterial spent cultures could inhibit the growth of the two pathogens. The results suggest that the co-presence of competitive bacteria, especially L. rhamnosus GG, significantly (p < 0.05) inhibited the growth of Salmonella and EHEC. Cell-free supernatants of L. rhamnosus GG cultures significantly reduced the pathogen populations in microbiological media. Although not as effective as L. rhamnosus GG in inhibiting the growth of Salmonella and EHEC, the biocontrol agents were more effective in competing for attachment to vegetable seeds. The study observed the inhibition of human bacterial pathogens by competitive bacteria or their metabolites and the competitive attachment to sprout seeds among all bacteria involved. The results will help strategize interventions to produce vegetable seeds and seed sprouts free of foodborne pathogens.
Sisymbrium Officinale (the Singers’ Plant) as an Ingredient: Analysis of Somatosensory Active Volatile Isothiocyanates in Model Food and Drinks
Authors: De Nisi Patrizia , Borgonovo Gigliola , Tramontana Samuele , Grassi Silvia , Picozzi Claudia , Scaglioni Leonardo , Mazzini Stefania , Mangieri Nicola , Bassoli Angela
Published: February 2021

Sisymbrium officinale (L.) Scop. (hedge mustard) is a wild common plant of the Brassicaceae family. It is known as “the singers’ plant” for its traditional use in treating aphonia and vocal disability. The plant is rich in glucosinolates and isothiocyanates; the latter has been demonstrated to be a strong agonist in vitro of the Transient Receptor Potential Ankirine 1 (TRPA1) channel, which is involved in the somatosensory perception of pungency as well as in the nociception pathway of inflammatory pain. Volatile ITCs are released by the enzymatic or chemical hydrolysis of GLSs (glucosinolates) during sample crushing and/or by the mastication of fresh plant tissues when the plant is used as an ingredient. Some functional food and drink model preparations have been realised: honey enriched with seeds and flowers, infusions, cold drink (voice drink), artisanal beer, and a fermented tea (kombucha). Using SPME-GCMS chromatography, we analysed samples of the plant and of the food preparations adopting conditions that simulate the release of isothiocyanates (ITCs) during oral assumption. Two active compounds, iso-propylisothiocyanate and 2-butylisothiocyanate, have been assayed. The concentration of ITCs varies according to temperature, pH, grinding conditions, and different plant organs used. Kombucha-type fermentation seems to eliminate the ITCs, whereas they are retained in beer. The ITCs’ concentration is higher when entire seeds and flowers are used.
Peroxidized Linoleic Acid, 13-HPODE, Alters Gene Expression Profile in Intestinal Epithelial Cells
Authors: Faizo Nisreen , Narasimhulu Chandrakala Aluganti , Forsman Anna , Yooseph Shibu , Parthasarathy Sampath
Published: February 2021

Lipid peroxides (LOOHs) abound in processed food and have been implicated in the pathology of diverse diseases including gut, cardiovascular, and cancer diseases. Recently, RNA Sequencing (RNA-seq) has been widely used to profile gene expression. To characterize gene expression and pathway dysregulation upon exposure to peroxidized linoleic acid, we incubated intestinal epithelial cells (Caco-2) with 100 µM of 13-hydroperoxyoctadecadienoic acid (13-HPODE) or linoleic acid (LA) for 24 h. Total RNA was extracted for library preparation and Illumina HiSeq sequencing. We identified 3094 differentially expressed genes (DEGs) in 13-HPODE-treated cells and 2862 DEGs in LA-treated cells relative to untreated cells. We show that 13-HPODE enhanced lipid metabolic pathways, including steroid hormone biosynthesis, PPAR signaling, and bile secretion, which alter lipid uptake and transport. 13-HPODE and LA treatments promoted detoxification mechanisms including cytochrome-P450. Conversely, both treatments suppressed oxidative phosphorylation. We also show that both treatments may promote absorptive cell differentiation and reduce proliferation by suppressing pathways involved in the cell cycle, DNA synthesis/repair and ribosomes, and enhancing focal adhesion. A qRT-PCR analysis of representative DEGs validated the RNA-seq analysis. This study provides insights into mechanisms by which 13-HPODE alters cellular processes and its possible involvement in mitochondrial dysfunction-related disorders and proposes potential therapeutic strategies to treat LOOH-related pathologies.
Prediction of Physicochemical Properties in Honeys with Portable Near-Infrared (microNIR) Spectroscopy Combined with Multivariate Data Processing
Authors: Escuredo Olga , Rodríguez-Flores María Shantal , Meno Laura , Seijo María Carmen
Published: February 2021

There is an increase in the consumption of natural foods with healthy benefits such as honey. The physicochemical composition contributes to the particularities of honey that differ depending on the botanical origin. Botanical and geographical declaration protects consumers from possible fraud and ensures the quality of the product. The objective of this study was to develop prediction models using a portable near-Infrared (MicroNIR) Spectroscopy to contribute to authenticate honeys from Northwest Spain. Based on reference physicochemical analyses of honey, prediction equations using principal components analysis and partial least square regression were developed. Statistical descriptors were good for moisture, hydroxymethylfurfural (HMF), color (Pfund, L and b* coordinates of CIELab) and flavonoids (RSQ > 0.75; RPD > 2.0), and acceptable for electrical conductivity (EC), pH and phenols (RSQ > 0.61; RDP > 1.5). Linear discriminant analysis correctly classified the 88.1% of honeys based on physicochemical parameters and botanical origin (heather, chestnut, eucalyptus, blackberry, honeydew, multifloral). Estimation of quality and physicochemical properties of honey with NIR-spectra data and chemometrics proves to be a powerful tool to fulfil quality goals of this bee product. Results supported that the portable spectroscopy devices provided an effective tool for the apicultural sector to rapid in-situ classification and authentication of honey.
Screening and Genetic Network Analysis of Genes Involved in Freezing and Thawing Resistance in DaMDHAR—Expressing Saccharomyces cerevisiae Using Gene Expression Profiling
Authors: Kim Il-Sup , Choi Woong , Son Jonghyeon , Lee Jun Hyuck , Lee Hyoungseok , Lee Jungeun , Shin Seung Chul , Kim Han-Woo
Published: February 2021

The cryoprotection of cell activity is a key determinant in frozen-dough technology. Although several factors that contribute to freezing tolerance have been reported, the mechanism underlying the manner in which yeast cells respond to freezing and thawing (FT) stress is not well established. Therefore, the present study demonstrated the relationship between DaMDHAR encoding monodehydroascorbate reductase from Antarctic hairgrass Deschampsia antarctica and stress tolerance to repeated FT cycles (FT2) in transgenic yeast Saccharomyces cerevisiae. DaMDHAR-expressing yeast (DM) cells identified by immunoblotting analysis showed high tolerance to FT stress conditions, thereby causing lower damage for yeast cells than wild-type (WT) cells with empty vector alone. To detect FT2 tolerance-associated genes, 3′-quant RNA sequencing was employed using mRNA isolated from DM and WT cells exposed to FT (FT2) conditions. Approximately 332 genes showed ≥2-fold changes in DM cells and were classified into various groups according to their gene expression. The expressions of the changed genes were further confirmed using western blot analysis and biochemical assay. The upregulated expression of 197 genes was associated with pentose phosphate pathway, NADP metabolic process, metal ion homeostasis, sulfate assimilation, β-alanine metabolism, glycerol synthesis, and integral component of mitochondrial and plasma membrane (PM) in DM cells under FT2 stress, whereas the expression of the remaining 135 genes was partially related to protein processing, selenocompound metabolism, cell cycle arrest, oxidative phosphorylation, and α-glucoside transport under the same condition. With regard to transcription factors in DM cells, MSN4 and CIN5 were activated, but MSN2 and MGA1 were not. Regarding antioxidant systems and protein kinases in DM cells under FT stress, CTT1, GTO, GEX1, and YOL024W were upregulated, whereas AIF1, COX2, and TRX3 were not. Gene activation represented by transcription factors and enzymatic antioxidants appears to be associated with FT2-stress tolerance in transgenic yeast cells. RCK1, MET14, and SIP18, but not YPK2, have been known to be involved in the protein kinase-mediated signalling pathway and glycogen synthesis. Moreover, SPI18 and HSP12 encoding hydrophilin in the PM were detected. Therefore, it was concluded that the genetic network via the change of gene expression levels of multiple genes contributing to the stabilization and functionality of the mitochondria and PM, not of a single gene, might be the crucial determinant for FT tolerance in DaMDAHR-expressing transgenic yeast. These findings provide a foundation for elucidating the DaMDHAR-dependent molecular mechanism of the complex functional resistance in the cellular response to FT stress.
Spindle Cell Hemangioma and Atypically Localized Juxtaglomerular Cell Tumor in a Patient with Hereditary BRIP1 Mutation: A Case Report
Authors: Papez Jan , Starha Jiri , Zerhau Pavel , Pavlovska Denisa , Jezova Marta , Jurencak Tomas , Slaba Katerina , Sterba Martin , Kerekes Arpad , Merta Tomas , Haluskova Terezia , Palova Hana , Slaby Ondrej , Sterba Jaroslav , Jabandziev Petr
Published: February 2021

Spindle cell hemangioma is a benign vascular tumor typically occurring in the dermis or subcutis of distal extremities as red–brown lesions that can grow in both size and number over time. They can be very painful and potentially disabling. A family history of cancer or previous history may be relevant and must be taken into consideration. Juxtaglomerular cell tumor (reninoma) is an extremely rare cause of secondary hypertension diagnosed mostly among adolescents and young adults. Excessive renin secretion results in secondary hyperaldosteronism. Subsequent hypokalemia and metabolic alkalosis, together with high blood pressure, are clues for clinical diagnosis. Histological examination of the excised tumor leads to a definitive diagnosis. Reninoma is found in subcapsular localization, in most cases as a solitary mass, in imaging studies of kidneys. Exceptionally, it can be located in another part of a kidney. Both spindle cell hemangioma and reninoma are extremely rare tumors in children and adolescents. Herein, the authors present a case report of a patient with hereditary BRCA1 interacting protein C-terminal helicase 1 (BRIP1) mutation, spindle cell hemangioma, and secondary hypertension caused by atypically localized reninoma.
Postmemory and Implication: Susanne Fritz Revisits the Post/War Period in Wie kommt der Krieg ins Kind (2018)
Authors: Eigler Friederike
Published: January 2021

After providing an introduction to German language family narratives of the past forty years and discussing the relevance of Michael Rothberg’s notion of the “Implicated Subject” for the study of these narratives, this article presents a detailed analysis of Susanne Fritz’s German-Polish family history Wie kommt der Krieg ins Kind (How does the war get into the child, 2018). Exemplifying the archival turn in postmemorial writings, the book draws on multiple sources and makes a compelling case for a broader public acknowledgment of the incarceration of German civilians (including the author’s mother) in post-war Polish labor camps, to this day a little-known aspect of German wartime suffering. The article examines on the one hand the intertwined nature of the mother’s wartime memories and the daughter’s postmemories and, on the other, questions of “implication” at the historical and the textual level (i.e., regarding the ancestors’ involvement in Nazi Germany and regarding the narrator’s positioning vis-à-vis her family history). The central challenge the narrative grapples with is how the suffering of Germans can be addressed within a larger perpetrator heritage. In its critical examination of archival materials and its multi-faceted examination of implication, the book makes a significant contribution to the collective memory of the (post-) war period as well as to the academic study of memory.
Bioelectrical Impedance Versus Biochemical Analysis of Hydration Status: Predictive Value for Prolonged Hospitalisation and Poor Discharge Destination for Older Patients
Authors: Wood Adrian D. , Edward Gillian D. , Cumming Kirsten , Kafri Mohannad W. , Soiza Roy L. , Hooper Lee , Potter John F. , Myint Phyo K.
Published: February 2021

Dehydration is prevalent in hospitalised patients and is associated with increased morbidity and mortality, particularly among the elderly (≥65 years). We aimed at comparing the performance of intracellular water to extracellular water ratio (ICW/ECW), calculated through a bioelectrical impedance analysis (BIA) of blood urea nitrogen, with the creatinine ratio (BUN/Cr) to predict poor outcomes in a cohort of prospectively identified patients. Data were combined from a cohort of elderly patients (≥65 years) admitted to hospital with fragility fracture (n = 125) and older adults aged ≥50 years admitted to hospital with stroke (n = 40). The association between hydration status and study outcomes (unfavourable discharge destination (rehabilitation, another ward, or death) and prolonged hospitalisation (>10 days)) was examined using logistic regression. The overall diagnostic accuracy of each hydration status measurement was assessed using the area under the receiver operating characteristic (ROC) curve. In 165 participants (mean age (SD) of 76.7 (9.2) years), an ICW/ECW ratio below the 25th percentile was associated with increased odds of poor discharge destination (OR (95% CI) = 4.25 (1.59–11.34)). Neither the relationship between the BUN/Cr ratio and prolonged stay nor discharge destination was significant. A BIA could be used utilised in conjunction with biochemical measurements to inform patient prognosis.
Assessing the Relationship between Helicobacter pylori and Chronic Kidney Disease
Authors: Hata Koichi , Koyama Teruhide , Ozaki Etsuko , Kuriyama Nagato , Mizuno Shigeto , Matsui Daisuke , Watanabe Isao , Uehara Ritei , Watanabe Yoshiyuki
Published: February 2021

The relationship between Helicobacter pylori infection and/or gastric disorders and chronic kidney disease (CKD) has not been elucidated. We investigated the relationship between Helicobacter pylori and/or atrophic gastritis (AG) and chronic kidney disease. In total, 3560 participants (1127 men and 2433 women) were eligible for this cross-sectional study. We divided participants into four study groups: with/without Helicobacter pylori infection and with/without AG. The HP (+) AG (−) group demonstrated a significant association with CKD compared with the HP (−) AG (−) group (adjusted odds ratio, 1.443; 95% confidence interval, 1.047–1.989). In contrast, the HP (+) AG (+) group showed significantly lower adjusted odds of CKD than the HP (−) AG (−) group (adjusted odds ratio, 0.608; 95% confidence interval, 0.402–0.920). H. pylori infection without AG might be associated with CKD in these participants. Conversely, the HP (+) AG (+) group had lower odds of CKD. Uncovering an association between gastric and renal conditions could lead to development of new treatment strategies.
The Quality of Carer–Patient Relationship Scale: Adaptation and Validation into Portuguese
Authors: Silva Rosa , Bobrowicz-Campos Elzbieta , Santos-Costa Paulo , Gil Isabel , Neves Hugo , Apóstolo João
Published: January 2021

Background: This study aimed to translate and adapt the Quality of the Carer–Patient Relationship (QCPR) scale into Portuguese and analyse both its psychometric properties and correlation with sociodemographic and clinical variables. Methods: Phase (1) Translate and culturally adapt the scale. Phase (2) Assess the scale’s confirmatory factorial analysis, internal consistency, construct validity, and correlations. Results: The experts classified the overall quality of the translation as adequate. A total of 53 dyads (cared-for person and carer) were assessed. In both versions, measures of central tendency and symmetry were also adequate, and the two factors under investigation had appropriate reliability, although in the conflict/critical factor, this was more fragile. Cronbach’s alpha values were 0.89 for the cared-for person version and 0.91 for the carer version. Conclusions: The QCPR scale showed satisfactory to good values of reliability. The assessment is essential to guarantee structured interventions by health professionals, since the quality of the dyads’ relationship seems to influence both older adults’ quality of life and carers’ health status. This study is a significant contribution to the introduction of the QCPR scale in the Portuguese clinical and scientific culture but also an opportunity to increase its use internationally.
Is the Occurrence of the Sticking Region in Maximum Smith Machine Squats the Result of Diminishing Potentiation and Co-Contraction of the Prime Movers among Recreationally Resistance Trained Males?
Authors: van den Tillaar Roland , Kristiansen Eirik Lindset , Larsen Stian
Published: February 2021

This study compared the kinetics, barbell, and joint kinematics and muscle activation patterns between a one-repetition maximum (1-RM) Smith machine squat and isometric squats performed at 10 different heights from the lowest barbell height. The aim was to investigate if force output is lowest in the sticking region, indicating that this is a poor biomechanical region. Twelve resistance trained males (age: 22 ± 5 years, mass: 83.5 ± 39 kg, height: 1.81 ± 0.20 m) were tested. A repeated two-way analysis of variance showed that Force output decreased in the sticking region for the 1-RM trial, while for the isometric trials, force output was lowest between 0–15 cm from the lowest barbell height, data that support the sticking region is a poor biomechanical region. Almost all muscles showed higher activity at 1-RM compared with isometric attempts (p < 0.05). The quadriceps activity decreased, and the gluteus maximus and shank muscle activity increased with increasing height (p ≤ 0.024). Moreover, the vastus muscles decreased only for the 1-RM trial while remaining stable at the same positions in the isometric trials (p = 0.04), indicating that potentiation occurs. Our findings suggest that a co-contraction between the hip and knee extensors, together with potentiation from the vastus muscles during ascent, creates a poor biomechanical region for force output, and thereby the sticking region among recreationally resistance trained males during 1-RM Smith machine squats.
Understanding Vocational Students’ Motivation for Dietary and Physical Activity Behaviors
Authors: Kuipers Annabelle , Kloek Gitte C. , de Vries Sanne I.
Published: February 2021

Unhealthy eating behaviors and low levels of physical activity are major problems in adolescents and young adults in vocational education. To develop effective intervention programs, more research is needed to understand how different types of motivation contribute to health behaviors. In the present study, Self-Determination Theory is used to examine how motivation contributes to dietary and physical activity behaviors in vocational students. This cross-sectional study included 809 students (mean age 17.8 ± 1.9 years) attending vocational education in the Netherlands. Linear multilevel regression analyses were used to investigate the association between types of motivation and dietary and physical activity behaviors. Amotivation was negatively associated with breakfast frequency and positively associated with diet soda consumption and high-calorie between-meal snacks. A positive association was found between autonomous motivation and water intake, breakfast frequency, fruit intake, and moderate-to-vigorous physical activity. Autonomous motivation was negatively associated with the consumption of unhealthy products. Controlled motivation was not associated with physical activity or dietary behaviors. Different types of motivation seem to explain either healthy or unhealthy dietary behaviors in vocational students. Autonomous motivation, in particular, was shown to be associated with healthy behaviors and could therefore be a valuable intervention target.
Heart Rate Variability and Physical Demands of In-Season Youth Elite Soccer Players
Authors: Sánchez-Sánchez Javier , Botella Javier , Felipe Hernández Jose Luis , León Manuel , Paredes-Hernández Víctor , Colino Enrique , Gallardo Leonor , García-Unanue Jorge
Published: February 2021

Monitoring fatigue and performance is important for adjusting training loads in soccer. Therefore, knowing the status of the player when applying a training stimulus is key to optimizing the players’ development. This study aims to evaluate the interaction between internal and external load, during training and matches, in an elite youth soccer team. Methods: seventeen youth players of the highest Spanish category were monitored with GPS devices during training and matches, as well as recording their nocturnal heart rate variability (HRV). We employed a linear mixed model to assess the physical demands between training and matches, and to compare the HRV variables. Results: a higher total distance (+2993.35–5746.56 m; ES = 1.4), distance at high intensity (+641.24–1907 m; ES = 1.5), sprint distance (+350.46–795.05 m; ES = 2.1), number of sprints (+18.38–41.58; ES = 1.9), and number of repeated sprints (+5.91–15.30; ES = 1.7) (all p < 0.001), but not in the number of accelerations, were reported during the matches when compared to the training sessions during the 11 weeks. The analysis of the HRV variables showed no significant differences between the accumulated values during a training week, providing similar results pre-match or post-match (p > 0.05). The LF/HFRATIO showed a negative influence on the total distance ran, distance at high intensity, distance in sprint, number of sprints, and repeated sprint. RRMEAN was positively related to the sprint number. Conclusion: the results of the present study suggest that nocturnal HRV variables are not different between pre-match and post-match. Furthermore, it suggests that LF/HFRATIO and RRMEAN during pre-match can determine the external load that the player will be able to complete during the match.
Occupational Stress in Spanish Police Officers: Validating the Effort-Reward Imbalance Questionnaire
Authors: Luceño-Moreno Lourdes , Talavera-Velasco Beatriz , Jaén-Díaz Marian , Martín-García Jesús
Published: February 2021

The Effort-Reward Imbalance Questionnaire (hereinafter, ERIQ) has been largely used worldwide to assess job stress, but it has not yet been applied in Spanish police. The objective of this study was to examine the construct validity and the internal consistency of the ERIQ in police officers. A cross-sectional study was carried out, using a nonprobability sampling (quota). A total of 217 Spanish police officers participated, 192 men (88.47%) and 25 women (11.53%). The mean age was 41 years (SD = 7.51). These police officers completed the ERIQ together with some other questionnaires (DECORE-21, MBI, GHQ and STAI) in order to provide evidence for validity based on the relationships to other constructs. A confirmatory factor analysis was performed and a matrix of correlations with the rest of constructs was created. The results showed an appropriate fit to the original model consisting of three scales. In addition, the scales of the ERIQ presented the expected relationship with the other constructs. The ERIQ is a valid instrument for assessing occupational stress in Spanish police officers and can improve the interventions in this professional group.
Validation and Screening Capacity of the European Portuguese Version of the SUNFRAIL Tool for Community-Dwelling Older Adults
Authors: Cardoso Ana Filipa , Bobrowicz-Campos Elzbieta , Teixeira-Santos Luísa , Cardoso Daniela , Couto Filipa , Apóstolo João
Published: February 2021

Early detection of frailty may prevent or delay adverse health outcomes in community-dwelling older adults. In Portugal, there are currently no valid multidimensional frailty screening tools. SUNFRAIL is a user-friendly multidimensional tool for frailty screening that can be used in primary care. Aims: (i) to determine the validity and reliability of the European Portuguese version of the SUNFRAIL tool for use in community-dwelling older adults; (ii) to assess the screening capacity of this version of SUNFRAIL using Fried’s phenotypic model criteria for frailty as a reference test. Methods: Cross-sectional pilot study in a convenience sample of 128 community-dwelling older adults. Objective and subjective data were collected. Internal consistency, concurrent validity, sensitivity, and specificity (ROC curve analysis) were examined. Results: Internal consistency was low. Significant moderate to strong correlations were found between different domains and the total score. The differences between robust, pre-frail, and frail older adults were significant. SUNFRAIL was also correlated with multimorbidity. Sensitivity and specificity were satisfactory. Conclusions: The European Portuguese version of the SUNFRAIL tool is a promising frailty screening tool for community-dwelling older adults to be routinely used in clinical practice. However, more consistent results on its validity and reliability are needed to be used nationwide.
The Reliability and Feasibility of the HESPER Web to Assess Perceived Needs in a Population Affected by a Humanitarian Emergency
Authors: Hugelius Karin , Nandain Charles , Semrau Maya , Holmefur Marie
Published: February 2021

Needs assessment is essential in the humanitarian response, and perceived needs can be associated with the levels of health in populations affected by humanitarian emergencies. This study aimed to evaluate the reliability and feasibility of The Humanitarian Emergency Settings Perceived Needs Web (HESPER Web) in a humanitarian context and to compare perceived needs of a random walk study sample with a self-selected study sample recruited though social media. The study context was the Dadaab refugee camp in Kenya. An alternate forms reliability evaluation and a feasibility evaluation was conducted. In total, 308 refugees participated in the study. HESPER Web was found to be reliable and usable for assessing needs, with an intraclass correlation coefficient (ICC) of 0.88, Cohen’s κ between 0.43 and 1.0 and a first priority need rating match of 81%. The HESPER Web was positively experienced, and the self-recruited study sample reported similar levels of needs and similar demographics as the randomized sample. The participants reported several unmet needs. HESPER Web offers a reliable tool for needs assessment in humanitarian emergencies where web-based surveys are considered as practical and suitable. It offers new possibilities for conducting remote assessments and research studies that include humanitarian populations that are rarely included in such evaluations.
The New Environmental Health in Australia: Failure to Launch?
Authors: Smith James C. , Whiley Harriet , Ross Kirstin E.
Published: February 2021

Background: The New Environmental Health is an approach to environmental health adopted in 1999. The new approach was in response to emerging health risks from the pressures that development placed on the environment, climate change, and increasing vulnerabilities of local communities. The new approach heralded a change in perception and roles within environmental health. Twenty years on, it seems these changes have not been embraced by local government. Methods: To determine whether this was the case, we assessed the use of the term “environmental health” in local government annual reports, and where environmental health functions sit within the organisational structure of councils. Results: We found that the New Environmental Health has not been adopted by councils and environmental health relates solely to the delivery of statutory services and legislative compliance. Conclusions: One result of this is local environmental health practitioners, who constitute the major health protection capability of councils, are defined by the narrow legislative obligations imposed on councils. This represents a significant lost opportunity as public health is not protected in the way that was envisaged with the adoption of the New Environmental Health.
Impact of Land Urbanization on Carbon Emissions in Urban Agglomerations of the Middle Reaches of the Yangtze River
Authors: Zhang Di , Wang Zhanqi , Li Shicheng , Zhang Hongwei
Published: February 2021

The urban agglomerations in the middle reaches of the Yangtze River (MYR-UA) are facing a severe challenge in reducing carbon emissions while maintaining stable economic growth and prioritizing ecological protection. The energy consumption related to land urbanization makes an important contribution to the increase in carbon emissions. In this study, an IPAT/Kaya identity model is used to understand how land urbanization affected carbon emissions in Wuhan, Changsha, and Nanchang, the three major cities in the middle reaches of the Yangtze River, from 2000 to 2017. Following the core idea of the Kaya identity model, sources of carbon emissions are decomposed into eight factors: urban expansion, economic level, industrialization, population structure, land use, population density, energy intensity, and carbon emission intensity. Furthermore, using the Logarithmic Mean Divisia Index (LMDI), we analyze how the different time periods and time series driving forces, especially land urbanization, affect regional carbon emissions. The results indicate that the total area of construction land and the total carbon emissions increased from 2000 to 2017, whereas the growth in carbon emissions decreased later in the period. Energy intensity is the biggest factor in restraining carbon emissions, followed by population density. Urban expansion is more significant than economic growth in promoting carbon emissions, especially in Nanchang. In contrast, the carbon emission intensity has little influence on carbon emissions. Changes in population structure, industrial level, and land use vary regionally and temporally over the different time period.
Editorial on the Special Issue on “Mountain Sports Activities: Injuries and Prevention”
Authors: Burtscher Martin , Niedermeier Martin , Gatterer Hannes
Published: February 2021

Recreational outdoor activities like mountain sports are gaining large popularity all over the world and particularly in the Alpine regions [...]
The Association between Participation in Organized Physical Activity and the Structure of Weekly Physical Activity in Polish Adolescents
Authors: Groffik Dorota , Frömel Karel , Ziemba Mateusz , Mitáš Josef
Published: February 2021

The main aim of the study was to determine the associations of participation in organized physical activity (OPA), and the structure of weekly physical activity (PA) with meeting the PA recommendations among Polish boys and girls. The research was conducted between 2012 and 2019 in the Silesian region of Poland among 3499 secondary school students. To determine the structure of PA (school, transportation, home, recreation, vigorous moderate, and walking), the International Physical Activity Questionnaire-Long Form (IPAQ-LF) questionnaire was used. Adolescents participating in OPA showed significantly more PA (p < 0.001) than non-participating adolescents. The strongest associations were observed between participation in OPA and vigorous PA. The weekly recommendation of vigorous PA was met by 61% of the students with three or more lessons of OPA per week, 29% of students with one or two lessons of OPA per week, and 24% of students not participating in OPA. Therefore, boys and girls with no OPA are at greatest risk of health issues. Schools, sports clubs, and leisure institutions should increase the participation of adolescents in OPA, especially non-participants. Comprehensive school PA programs should especially include those forms of OPA that respect health weaknesses, individual talents for specific types of PA, and preferred types of PA among adolescents.
Effects of a Clinical Simulation Course about Basic Life Support on Undergraduate Nursing Students’ Learning
Authors: Requena-Mullor María del Mar , Alarcón-Rodríguez Raquel , Ventura-Miranda María Isabel , García-González Jessica
Published: February 2021

Training in basic life support (BLS) using clinical simulation improves compression rates and the development of cardiopulmonary resuscitation (CPR) skills. This study analyzed the learning outcomes of undergraduate nursing students taking a BLS clinical simulation course. A total of 479 nursing students participated. A pre-test and post-test were carried out to evaluate theoretical knowledge of BLS through questions about anatomical physiology, cardiac arrest, the chain of survival, and CPR. A checklist was used in the simulation to evaluate practical skills of basic CPR. The learning outcomes showed statistically significant differences in the total score of the pre-test and after completing the BLS clinical simulation course (pre-test: 12.61 (2.30), post-test: 15.60 (2.06), p < 0.001). A significant increase in the mean scores was observed after completing the course in each of the four parts of the assessment protocol (p < 0.001). The increase in scores in the cardiac arrest and CPR sections were relevant (Rosenthal’s r: −0.72). The students who had prior knowledge of BLS scored higher on both the pre-test and the post-test. The BLS simulation course was an effective method of teaching and learning BLS skills.
Study for the Design of a Protocol to Assess the Impact of Stress in the Quality of Life of Workers
Authors: Concheiro-Moscoso Patricia , Groba Betania , Martínez-Martínez Francisco José , Miranda-Duro María del Carmen , Nieto-Riveiro Laura , Pousada Thais , Queirós Cristina , Pereira Javier
Published: February 2021

(1) Background: Work stress is one of the most relevant issues in public health. It has a significant impact on health, especially the development of mental disorders, causing occupational imbalance. There is a growing interest in the development of tools with a positive effect on workers. To this end, wearable technology is becoming increasingly popular, as it measures biometric variables like heartbeat, activity, and sleep. This information may be used to assess the stress a person is suffering, which could allow the development of stress coping strategies, both at a professional and personal level. (2) Methods: This paper describes an observational, analytical, and longitudinal study which will be set at a research center in A Coruña, Spain. Various scales and questionnaires will be filled in by the participants throughout the study. For the statistical analysis, specific methods will be used to evaluate the association between numerical and categorical variables. (3) Discussion: This study will lay the foundation for a bigger, more complete study to assess occupational stress in different work environments. This will allow us to begin to understand how occupational stress influences daily life activity and occupational balance, which could directly enhance the quality of life of workers if the necessary measures are taken.
Dating Violence Victimization among Adolescents in Europe: Baseline Results from the Lights4Violence Project
Authors: Vives-Cases Carmen , Sanz-Barbero Belén , Ayala Alba , Pérez-Martínez Vanesa , Sánchez-SanSegundo Miriam , Jaskulska Sylwia , Antunes das Neves Ana Sofia , Forjaz Maria João , Pyżalski Jacek , Bowes Nic , Costa Dália , Waszyńska Katarzyna , Jankowiak Barbara , Mocanu Veronica , Davó-Blanes María Carmen
Published: February 2021

Dating violence (DV) among adolescents is a public health issue because of its negative health consequences. In this study, we aimed to analyse the prevalence and the psychosocial and socioeconomic risk and protective factors associated DV among male and female adolescents in Europe. It was performed a cross-sectional study based on a non-probabilistic sample of 1555 students aged 13–16 years (2018–2019). The global prevalence of DV victimization was significantly greater among girls than boys (girls: 34.1%, boys: 26.7%; p = 0.012). The prevalence of DV in both girls and boys was greater for those over age 15 (girls: 48.5% p < 0.001; boys: 35.9%; p = 0.019). There was an increased likelihood of DV victimization among girls whose fathers did not have paid employment (p = 0.024), who suffered abuse in childhood, and reported higher Benevolent Sexism [PR (CI 95%): 1.01 (1.00–1.03)] and machismo [1.02 (1.00–1.05)]. In the case of boys, the likelihood of DV increased with abuse in childhood (p = 0.018), lower parental support [0.97 (0.96–0.99)], high hostile sexism scores (p = 0.019), lower acceptance of violence (p = 0.009) and high machismo (p < 0.001). Abuse in childhood was shown to be the main factor associated with being a victim of DV in both population groups, as well as sexism and machismo attitudes. These results may contribute to future DV prevention school programs for both, teenagers and children of elementary school ages.
Joint Promotion Partner Recommendation Systems Using Data from Location-Based Social Networks
Authors: Chen Yi-Chung , Huang Hsi-Ho , Chiu Sheng-Min , Lee Chiang
Published: January 2021

Joint promotion is a valuable business strategy that enables companies to attract more customers at lower operational cost. However, finding a suitable partner can be extremely difficult. Conventionally, one of the most common approaches is to conduct survey-based analysis; however, this method can be unreliable as well as time-consuming, considering that there are likely to be thousands of potential partners in a city. This article proposes a framework to recommend Joint Promotion Partners using location-based social networks (LBSN) data. We considered six factors in determining the suitability of a partner (customer base, association, rating and awareness, prices and star ratings, distance, and promotional strategy) and developed efficient algorithms to perform the required calculations. The effectiveness and efficiency of our algorithms were verified using the Foursquare dataset and real-life case studies.
Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve
Authors: D. Alvites Rui , V. Branquinho Mariana , Sousa Ana C. , Zen Federica , Maurina Monica , Raimondo Stefania , Mendonça Carla , Atayde Luís , Geuna Stefano , Varejão Artur S.P. , Maurício Ana C.
Published: January 2021

Thousands of people worldwide suffer from peripheral nerve injuries and must deal daily with the resulting physiological and functional deficits. Recent advances in this field are still insufficient to guarantee adequate outcomes, and the development of new and compelling therapeutic options require the use of valid preclinical models that effectively replicate the characteristics and challenges associated with these injuries in humans. In this study, we established a sheep model for common peroneal nerve injuries that can be applied in preclinical research with the advantages associated with the use of large animal models. The anatomy of the common peroneal nerve and topographically related nerves, the functional consequences of its injury and a neurological examination directed at this nerve have been described. Furthermore, the surgical protocol for accessing the common peroneal nerve, the induction of different types of nerve damage and the application of possible therapeutic options were described. Finally, a preliminary morphological and stereological study was carried out to establish control values for the healthy common peroneal nerves regarding this animal model and to identify preliminary differences between therapeutic methods. This study allowed to define the described lateral incision as the best to access the common peroneal nerve, besides establishing 12 and 24 weeks as the minimum periods to study lesions of axonotmesis and neurotmesis, respectively, in this specie. The post-mortem evaluation of the harvested nerves allowed to register stereological values for healthy common peroneal nerves to be used as controls in future studies, and to establish preliminary values associated with the therapeutic performance of the different applied options, although limited by a small sample size, thus requiring further validation studies. Finally, this study demonstrated that the sheep is a valid model of peripheral nerve injury to be used in pre-clinical and translational works and to evaluate the efficacy and safety of nerve injury therapeutic options before its clinical application in humans and veterinary patients.
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression
Authors: Lee Hanon , Lim Jiyeong , Oh Jang-Hee , Cho Soyun , Chung Jin Ho
Published: January 2021

Proteoglycan (PG) is a glycosaminoglycan (GAG)-conjugated protein essential for maintaining tissue strength and elasticity. The most abundant skin PGs, biglycan and decorin, have been reported to decrease as skin ages. Insulin-like growth factor-1 (IGF-1) is important in various physiological functions such as cell survival, growth, and apoptosis. It is well known that the serum level of IGF-1 decreases with age. Therefore, we investigated whether and how IGF-1 affects biglycan and decorin. When primary cultured normal human dermal fibroblasts (NHDFs) were treated with IGF-1, protein levels of biglycan and decorin increased, despite no difference in mRNA expression. This increase was not inhibited by transcription blockade using actinomycin D, suggesting that it is mediated by IGF-1-induced enhanced translation. Additionally, both mRNA and protein expression of ADAMTS5, a PG-degrading enzyme, were decreased in IGF-1-treated NHDFs. Knockdown of ADAMTS5 via RNA interference increased protein expression of biglycan and decorin. Moreover, mRNA and protein expression of ADAMTS5 increased in aged human skin tissues compared to young tissue. Overall, IGF-1 increases biglycan and decorin, which is achieved by improving protein translation to increase synthesis and preventing ADAMTS5-mediated degradation. This suggests a new role of IGF-1 as a regulator for biglycan and decorin in skin aging process.
Multifaceted Role of Matrix Metalloproteinases in Neurodegenerative Diseases: Pathophysiological and Therapeutic Perspectives
Authors: Behl Tapan , Kaur Gagandeep , Sehgal Aayush , Bhardwaj Shaveta , Singh Sukhbir , Buhas Camelia , Judea-Pusta Claudia , Uivarosan Diana , Munteanu Mihai Alexandru , Bungau Simona
Published: January 2021

Neurodegeneration is the pathological condition, in which the nervous system or neuron loses its structure, function, or both, leading to progressive degeneration or the death of neurons, and well-defined associations of tissue system, resulting in clinical manifestations. Neuroinflammation has been shown to precede neurodegeneration in several neurodegenerative diseases (NDs). No drug is yet known to delay or treat neurodegeneration. Although the etiology and potential causes of NDs remain widely indefinable, matrix metalloproteinases (MMPs) evidently have a crucial role in the progression of NDs. MMPs, a protein family of zinc (Zn2+)-containing endopeptidases, are pivotal agents that are involved in various biological and pathological processes in the central nervous system (CNS). The current review delineates the several emerging evidence demonstrating the effects of MMPs in the progression of NDs, wherein they regulate several processes, such as (neuro)inflammation, microglial activation, amyloid peptide degradation, blood brain barrier (BBB) disruption, dopaminergic apoptosis, and α-synuclein modulation, leading to neurotoxicity and neuron death. Published papers to date were searched via PubMed, MEDLINE, etc., while using selective keywords highlighted in our manuscript. We also aim to shed a light on pathophysiological effect of MMPs in the CNS and focus our attention on its detrimental and beneficial effects in NDs, with a special focus on Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), Alzheimer’s disease (AD), multiple sclerosis (MS), and Huntington’s disease (HD), and discussed various therapeutic strategies targeting MMPs, which could serve as potential modulators in NDs. Over time, several agents have been developed in order to overcome challenges and open up the possibilities for making selective modulators of MMPs to decipher the multifaceted functions of MMPs in NDs. There is still a greater need to explore them in clinics.