bluuebunny/crossref_metadata_2025 huggingface dataset:
Sugar Antennae for Guidance Signals: Syndecans and Glypicans Integrate Directional Cues for Navigating Neurons
Authors: Rhiner Christa , Hengartner Michael O.
Published: May 2007

Attractive and repulsive signals guide migrating nerve cells in all directions when the nervous system starts to form. The neurons extend thin processes, axons, that connect over wide distances with other brain cells to form a complicated neuronal network. One of the most fascinating questions in neuroscience is how the correct wiring of billions of nerve cells in our brain is controlled. Several protein families are known to serve as guidance cues for navigating neurons and axons. Nevertheless, the combinatorial potential of these proteins seems to be insufficient to sculpt the entire neuronal network and the appropriate formation of connections. Recently, heparan sulfate proteoglycans (HSPGs), which are present on the cell surface of neurons and in the extracellular matrix through which neurons and axons migrate, have been found to play a role in regulating cell migration and axon guidance. Intriguingly, the large number of distinct modifications that can be put onto the sugar side chains of these PGs would in principle allow for an enormous diversity of HSPGs, which could help in regulating the vast number of guidance choices taken by individual neurons. In this review, we will focus on the role of the cell surface HSPGs syndecan and glypican and specific HS modifications in promoting neuronal migration, axon guidance, and synapse formation.
Collagen Fibril Diameter in Relation to Bone Site and to Calcium/Phosphorus Ratio
Authors: Berillis Panagiotis , Emfietzoglou Dimitris , Tzaphlidou Margaret
Published: May 2007

The collagen fibril diameter was measured in cortical bone samples from the femoral neck, rear and front tibia of female and male rats and rabbits using electron microscopy analysis. In most cases, statistically significant differences in mean fibril diameter values between different bone sites were detected. The order of magnitude for the above structural parameter was the same for both genders in both experimental species. In rats, the greatest mean diameter value was that for the femoral, while in rabbits, the one for the rear tibia demonstrating a dependence on bone use and life style. An important aspect was the agreement between these observations and the mean values for Ca/P ratio, as observed in previous experiments, in the same bone sites and animals. Collagen fibril diameter and Ca/P ratio can both serve as indexes of bone quality.
Diaphragm Disease: The Limitation of Laparoscopy and Assessment of the Small Bowel for Strictures Using a Ball Bearing
Authors: Moffat C.E. , Khyan M.N. , Davies C.G. , Ghauri A.S.K. , Ranaboldo C.J.
Published: May 2007

Diaphragm disease is a rare cause of intestinal obstruction that will be seen with increasing frequency with the widespread use of nonsteroidal anti-inflammatory drugs (NSAIDs). We present a case study of a patient with diaphragm disease where the diagnosis was not apparent at laparoscopy, and passage of a steel ball through the small intestine was required to identify all strictures present. A high index of suspicion, recognition of the limitations of conventional diagnostic aids, and the need to assess the full length of the small bowel are all important in the surgical management of this condition.
Double Anonymity and the Peer Review Process
Authors: Brown Richard J.C.
Published: May 2007

The process of peer review for submissions to scientific journals is a well-established and widely used procedure. Review by one's peers is a well-recognised and long-standing method of appraisal. Throughout all branches of science, medicine, humanities, art, literature, politics, sport, and in fact almost all areas of human endeavour, the judgement of work by an individual or group of experts in similar fields of study is the most rigorous and valuable form of recognition. “Peer review”, as this process is commonly known, is an important method of assuring quality, relevance and novelty of work. However, is there still room for improvement in the procedural aspects of peer review?
Androgen Excess Disorders in Women: The Severe Insulin-Resistant Hyperandrogenic Syndrome, HAIR-AN
Authors: Rager Kristin M. , Omar Hatim A.
Published: May 2007

HAIR-AN syndrome (hyperandrogenism, insulin resistance, acanthosis nigricans) is a subset of the polycystic ovary syndrome, where the patients demonstrate severe insulin resistance. It is theorized that both genetic and environmental factors, such as obesity, give rise to the development of HAIR-AN. Diagnosis is primarily clinical, with laboratory values lending further support. Treatment is aimed at decreasing insulin resistance, regulating ovulation, and decreasing acne, acanthosis nigricans, and hirsutism.
Rett Syndrome. A Review with Emphasis on Clinical Characteristics and Intervention
Authors: Lotan Meir , Ben-Zeev Bruria
Published: May 2007

Rett syndrome (RS) is a genetic disorder affecting mainly females. In the majority of cases, it is caused by a mutation in MECP2, an X-linked gene, and considered the most common multidisabling genetic disorder in females after Down syndrome. This article is an introduction to RS. It presents the basic understanding of common characteristics typical of this disorder, and the variants from the classical expression of RS. The present article will review the current literature on RS, specially focusing on the clinical characteristics of the disorder. The intention of the article is to set a clear, up-to-date picture of the individual with RS to prepare the clinician for their future meetings with this population.
Using Protein Homology Models for Structure-Based Studies: Approaches to Model Refinement
Authors: Kairys V. , Gilson M.K. , Fernandes Miguel Xavier
Published: May 2007

Homology modeling is a computational methodology to assign a 3-D structure to a target protein when experimental data are not available. The methodology uses another protein with a known structure that shares some sequence identity with the target as a template. The crudest approach is to thread the target protein backbone atoms over the backbone atoms of the template protein, but necessary refinement methods are needed to produce realistic models. In this mini-review anchored within the scope of drug design, we show the validity of using homology models of proteins in the discovery of binders for potential therapeutic targets. We also report several different approaches to homology model refinement, going from very simple to the most elaborate. Results show that refinement approaches are system dependent and that more elaborate methodologies do not always correlate with better performances from built homology models.
Lipoxins and Annexin-1: Resolution of Inflammation and Regulation of Phagocytosis of Apoptotic Cells
Authors: Scannell Michael , Maderna Paola
Published: May 2007

Phagocytosis of apoptotic cells plays a pivotal role in developmental processes and in the resolution of inflammation. Failed or delayed clearance of apoptotic cells can result in chronic inflammation. Furthermore, clearance of apoptotic cells leads to release of anti-inflammatory cytokines. Recent evidence has shown that endogenous mediators can regulate such processes. In this article, we will review the recognition and signaling mechanisms involved in the phagocytosis of apoptotic cells as well as the role of endogenous compounds that play a relevant role in the modulation of inflammation. The first of these endogenous local mediators to be described are lipoxins (LXs). LXs and aspirin-triggered LXs (ATLs) are considered to act as “braking signals” in inflammation, limiting the entrance of leukocytes to the site of inflammation through inhibition of neutrophil and eosinophil trafficking. LXs are actively involved in resolution of inflammation, stimulating nonphlogistic phagocytosis of apoptotic cells by macrophages. LXA4and ATLs elicit cellular responses by interacting with a G protein -coupled receptor (ALXR) that is expressed in various cell types. ALXR, originally identified as a low-affinity N-formyl-methionyl-leucyl-phenylalanine receptor-like 1, can bind pleiotropic ligands, i.e., both lipid and peptides, including the glucocorticoid-inducible protein, annexin-1. Interestingly, a role for annexin-1 in phagocytosis has recently emerged. Understanding the role and mechanism of the powerful anti-inflammatory and proresolution actions of endogenous compounds can be a useful tool in the development of potential therapeutics in resolving inflammatory diseases.
The Digestive System and Nutritional Considerations for Individuals with Rett Syndrome
Authors: Lotan Meir , Zysman Lilit
Published: May 2007

Rett syndrome (RS) is a neurodevelopmental syndrome of genetic origin that mainly affects females. Individuals diagnosed with RS exhibit a variety of functional difficulties that impair their quality of life. One of the affected systems is the digestive system, where 74% of persons with RS have abnormal functioning. The affected digestive system causes this population to present an array of problems, such as gastroesophageal reflux (GER), constipation, and malnutrition, leading to failure to thrive (FTT), which resolves in reduced functional ability. Due to the severe effects of the dysfunctional digestive system of individuals with RS, this article will describe the problems common to this population, as well as propose some clinical suggestions for intervention. .
Integrated Confocal and Scanning Probe Microscopy for Biomedical Research
Authors: Haupt B.J. , Pelling A.E. , Horton M.A.
Published: May 2007

Atomic force microscopy (AFM) continues to be developed, not only in design, but also in application. The new focus of using AFM is changing from pure material to biomedical studies. More frequently, it is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy (CLSM) and fluorescent imaging, to provide a more comprehensive understanding of biological systems. To date, AFM has been used increasingly as a precise micromanipulator, probing and altering the mechanobiological characteristics of living cells and tissues, in order to examine specific, receptor-ligand interactions, material properties, and cell behavior. In this review, we discuss the development of this new hybrid AFM, current research, and potential applications in diagnosis and the detection of disease.
Perceived Parental Monitoring and Health Risk Behavior among Public Secondary School Students in El Salvador
Authors: Springer Andrew E. , Sharma Shreela , de Guardado Alba Margarita , Nava Francisco Vázquez , Kelder Steven H.
Published: May 2007

Although parental monitoring has received considerable attention in studies of U.S. adolescents, few published studies have examined how parents' knowledge of their children's whereabouts may influence health risk behaviors in adolescents living in Latin America. We investigated the association between perceived parental monitoring and substance use, fighting, and sexual behaviors in rural and urban Salvadoran adolescents (n = 982). After adjusting for several sociodemographic covariates, multilevel regression analyses indicated that students reporting low parental monitoring were between 2 to 3.5 times more likely to report risk behaviors examined. The promotion of specific parenting practices such as parental monitoring may hold promise for reducing adolescent risk behaviors in El Salvador.
Chromatin Modification and Remodeling in Heart Development
Authors: Delgado-Olguín Paul , Takeuchi Jun K. , Bruneau Benoit G.
Published: May 2007

In organogenesis, cell types are specified from determined precursors as morphogenetic patterning takes place. These events are largely controlled by tissue-specific transcription factors. These transcription factors must function within the context of chromatin to activate or repress target genes. Recent evidence suggests that chromatin-remodeling and -modifying factors may have tissue-specific function. Here we review the potential roles for chromatin-remodeling and -modifying proteins in the development of the mammalian heart.
Limb Regeneration inXenopus laevisFroglet
Authors: Suzuki Makoto , Yakushiji Nayuta , Nakada Yasuaki , Satoh Akira , Ide Hiroyuki , Tamura Koji
Published: May 2007

Limb regeneration in amphibians is a representative process of epimorphosis. This type of organ regeneration, in which a mass of undifferentiated cells referred to as the “blastema” proliferate to restore the lost part of the amputated organ, is distinct from morphallaxis as observed, for instance, in Hydra, in which rearrangement of pre-existing cells and tissues mainly contribute to regeneration. In contrast to complete limb regeneration in urodele amphibians, limb regeneration inXenopus, an anuran amphibian, is restricted. In this review of some aspects regarding adult limb regeneration inXenopus laevis, we suggest that limb regeneration in adultXenopus, which is pattern/tissue deficient, also represents epimorphosis.
Newt Opportunities for Understanding the Dedifferentiation Process
Authors: Chaar Ziad Y. , Tsilfidis Catherine
Published: May 2007

Urodele amphibians, such as the newtNotophthalmus viridescens, have the unique ability to regenerate limbs, spinal cord, eye structures, and many vital organs through a process called epimorphic regeneration. Although the cellular basis of regeneration has been studied in detail, we know relatively little about the molecular controls of the process. This review provides an overview of forelimb regeneration in the newt, addressing what we know about cellular and molecular aspects. Particular focus is placed on the dedifferentiation process, which yields a population of embryonic-like pluripotent cells that will eventually reform the lost structure. This cellular plasticity seems to be the key to regenerative ability. We discuss the dedifferentiation process in newt forelimb regeneration and outline the various studies that have revealed that mammalian cells also have the ability to dedifferentiate if given the appropriate triggers.
Clinical Holistic Medicine: The Dean Ornish Program (“Opening the Heart”) in Cardiovascular Disease
Authors: Ventegodt Søren , Merrick Efrat , Merrick Joav
Published: May 2007

Dean Ornish of the Preventive Medicine Research Institute in Sausalito, California has created an intensive holistic treatment for coronary heart patients with improved diet (low fat, whole foods, plant based), exercise, stress management, and social support that has proven to be efficient. In this paper, we analyze the rationale behind his cure in relation to contemporary holistic medical theory. In spite of a complex treatment program, the principles seem to be simple and in accordance with holistic medical theories, like the Antonovsky concept of rehabilitating the sense of coherence and the life mission theory for holistic medicine. We believe there is a need for the allocation of resources for further research into the aspects of holistic health and its methods, where positive and significant results have been proven and reproduced at several sites.
Histochemical Differential Diagnosis and Polarization Optical Analysis of Amyloid and Amyloidosis
Authors: Bély M.
Published: May 2007

Amyloidosis is characterized by extracellular deposition of protein fibrils of chemically heterogeneous composition. Early recognition and identification of amyloid deposits allows an early start of therapy, which may entail a better prognosis. Congo red staining according to Romhányi (1971) is a highly specific and sensitive method for early microscopic recognition of amyloidosis. The main and most important types of amyloidosis may be distinguished by classic histochemical methods of performate pretreatment according to Romhányi (1979), or by KMnO4oxidation according to Wright (1977) followed by Congo red staining and viewed under polarized light. Differences in the speed of breakdown (disintegration) of amyloid deposits according to Bély and Apáthy allow a more precise distinction of various types of amyloid.
Neuroscience of Meditation
Authors: Deshmukh Vinod D.
Published: May 2007

Dhyana-Yogais a Sanskrit word for the ancient discipline of meditation, as a means toSamadhior enlightenment. Samadhi is a self-absorptive, adaptive state with realization of ones being in harmony with reality. It is unitive, undifferentiated, reality-consciousness, an essential being, which can only be experienced by spontaneous intuition and self-understanding. Modern neuroscience can help us to better understand Dhyana-Yoga. This article discusses topics including brain-mind-reality, consciousness, attention, emotional intelligence, sense of self, meditative mind, and meditative brain. A new hypothesis is proposed for a better understanding of the meditative mind. Meditation is an art of being serene and alert in the present moment, instead of constantly struggling to change or to become. It is an art of efficient management of attentional energy with total engagement (poornata, presence, mindfulness) or disengagement (shunyata, silence, emptiness). In both states, there is an experience of spontaneous unity with no sense of situational interactive self or personal time. It is a simultaneous, participatory consciousness rather than a dualistic, sequential attentiveness. There is a natural sense of well being with self-understanding, spontaneous joy, serenity, freedom, and self-fulfillment. It is where the ultimate pursuit of happiness and the search for meaning of life resolve. One realizes the truth of ones harmonious being in nature and nature in oneself. It is being alive at its fullest, when each conscious moment becomes a dynamic process of discovery and continuous learning of the ever-new unfolding reality.
Process Evaluation of the Tier 1 Program of the Project P.A.T.H.S.
Authors: Shek Daniel T.L. , Ma Hing Keung , Lui Joyce H.Y. , Lung Daniel W.M.
Published: May 2007

To understand the implementation quality of the Tier 1 Program of the Project P.A.T.H.S., two observers carried out process evaluation in six schools randomly selected from the participating schools in the form of systematic observations of 12 units. Results showed that the overall level of program adherence was generally high, ranging from 50% to 95%, with an average of 84.5%. High implementation quality of the program in the areas of student interest, student participation and involvement, classroom control, use of interactive delivery method, use of strategies to enhance student motivation, instructors familiarity with the students, opportunity for reflection, degree of achievement of the objectives, quality of preparation, overall implementation quality, and success of implementation was also observed. The findings provide support for the implementation quality of the program.
Fixed Drug Eruption of the Penis Secondary to Sulfamethoxazole-Trimethoprim
Authors: Lawrentschuk Nathan , Pan David , Troy Andrew
Published: May 2007

Penile lesions are encountered in a variety of fields from family medicine practice through urology, to sexual health specialists. It is important that practitioners consider and recognize fixed drug eruptions of the penis while being able to initiate appropriate treatment in order to avoid misdiagnosis and avoidable stress. In summary, withdrawal of the offending medication and initiation of corticosteroid therapy remain the cornerstones of treatment of fixed drug eruptions of the penis.
Endothelin-2/Vasoactive Intestinal Contractor: Regulation of Expression via Reactive Oxygen Species Induced by CoCl22, and Biological Activities Including Neurite Outgrowth in PC12 Cells
Authors: Kotake-Nara Eiichi , Saida Kaname
Published: May 2007

This paper reviews the local hormone endothelin-2 (ET-2), or vasoactive intestinal contractor (VIC), a member of the vasoconstrictor ET peptide family, where ET-2 is the human orthologous peptide of the murine VIC. While ET-2/VIC gene expression has been observed in some normal tissues, ET-2 recently has been reported to act as a tumor marker and as a hypoxia-induced autocrine survival factor in tumor cells. A recently published study reported that the hypoxic mimetic agent CoCl2at 200 µM increased expression of the ET-2/VIC gene, decreased expression of the ET-1 gene, and induced intracellular reactive oxygen species (ROS) increase and neurite outgrowth in neuronal model PC12 cells. The ROS was generated by addition of CoCl2to the culture medium, and the CoCl2-induced effects were completely inhibited by the antioxidantN-acetyl cysteine. Furthermore, interleukin-6 (IL-6) gene expression was up-regulated upon the differentiation induced by CoCl2. These results suggest that expression of ET-2/VIC and ET-1 mediated by CoCl2-induced ROS may be associated with neuronal differentiation through the regulation of IL-6 expression. CoCl2acts as a pro-oxidant, as do Fe(II, III) and Cu(II). However, some biological activities have been reported for CoCl2that have not been observed for other metal salts such as FeCl3, CuSO4, and NiCl2. The characteristic actions of CoCl2may be associated with the differentiation of PC12 cells. Further elucidation of the mechanism of neurite outgrowth and regulation of ET-2/VIC expression by CoCl2may lead to the development of treatments for neuronal disorders.
Incisional Hernia in a 12-mm Nonbladed Trocar Site Following Laparoscopic Nephrectomy
Authors: Kouba Erik J. , Hubbard J. Slade , Wallen Eric , Pruthi Raj S.
Published: May 2007

Non-bladed trocars and radially dilating systems are considered less traumatic to the abdominal wall because they do not incise the fascia itself. Since the fascia is not cut, it has believed that the fascia closes by itself. Consequently, several authors have suggested that closure of the abdominal fascia may be unnecessary when such non-bladed laparoscopic trocars are used. We report of a case in which a port site hernia was diagnosed at the site of a 12 mm non-bladed trocar 11 days after laparoscopic nephrectomy. Although it may be true that in many cases port site closure is unnecessary and does not result in bowel herniation, this case along with a prior report serve as important reminders that port site hernias are possible even in the use of non-bladed or radial dilating systems, and that there exists a number of potential variables that may predispose to herniation and consequently the ability to predict such events in individual patients remains uncertain. As such, we recommend closing 10 mm or larger port sites irrespective of trocar design.
Superficial Granulomatous Pyoderma Gangrenosum of the Penis: A Case Report
Authors: Gopi Shyamala S. , Evans Alan T. , Raza Asif , Byrne Derek J.
Published: May 2007

Classic type of pyoderma gangrenosum (PG) is an uncommon ulceronecrotic cutaneous disease of uncertain aetiology characterised by broad zones of confluent ulceration with violaceous undermined margins. Some 50% of cases are associated with systemic diseases. The superficial granulomatous variant of pyoderma gangrenosum (SGPG) of the external genitalia is extremely rare Patients with this condition develop single or multiple ulcerated skin lesions often with sinus tract formation. The majority of these lesions were found on the trunk and limbs. SGPG is less likely to be associated with underlying disease processes than classic PG. We present a 58 year-old with recalcitrant penile ulceration demonstrated to be SGPG on biopsy. Although rare and poorly recognised, the histological features are sufficiently typical to allow the correct diagnosis to be established.
Anticholinesterase Toxicity and Oxidative Stress
Authors: Milatovic Dejan , Gupta Ramesh C. , Aschner Michael
Published: May 2007

Anticholinesterase compounds, organophosphates (OPs) and carbamates (CMs) are commonly used for a variety of purposes in agriculture and in human and veterinary medicine. They exert their toxicity in mammalian system primarily by virtue of acetylcholinesterase (AChE) inhibition at the synapses and neuromuscular junctions, leading into the signs of hypercholinergic preponderance. However, the mechanism(s) involved in brain/muscle damage appear to be linked with alteration in antioxidant and the scavenging system leading to free radical-mediated injury. OPs and CMs cause excessive formation of F2-isoprostanes and F4-neuroprostanes,in vivobiomarkers of lipid peroxidation and generation of reactive oxygen species (ROS), and of citrulline, a marker of NO/NOS and reactive nitrogen species (RNS) generation. In addition, during the course of these excitatory processes and inhibition of AChE, a high rate of ATP consumption, coupled with the inhibition of oxidative phosphorylation, compromise the cell's ability to maintain its energy levels and excessive amounts of ROS and RNS may be generated. Pretreatment withN-methyl D-aspartate (NMDA) receptor antagonist memantine, in combination with atropine sulfate, provides significant protection against inhibition of AChE, increases of ROS/RNS, and depletion of high-energy phosphates induced by DFP/carbofuran. Similar antioxidative effects are observed with a spin trapping agent, phenyl-N-tert-butylnitrone (PBN) or chain breaking antioxidant vitamin E. This review describes the mechanisms involved in anticholinesterase-induced oxidative/nitrosative injury in target organs of OPs/CMs, and protection by various agents.
Prostatic Adenosquamous Carcinoma Metastasizing to Testis
Authors: Baydar Dilek Ertoy , Kosemehmetoglu Kemal , Akdogan Bulent , Ozen Haluk
Published: May 2007

Adenosquamous carcinoma of the prostate is an unusual tumor with poor prognosis. Most arise after hormonal or radiotherapy of conventional prostatic adenocarcinoma. Sarcomatous transformation in them has been reported in only a few cases. Here, we present a unique case of “de novo prostatic adenosquamous carcinoma with focal sarcomatoid areas” that showed testicular metastasis, detected after scrotal orchiectomy.
Development of a Positive Youth Development Program: Promoting the Mental Health of Stressful Adolescents Using Principles of Problem Solving Therapy
Authors: Shek Daniel T.L. , Chow Joyce T.W.
Published: May 2007

This paper outlines the proposal for the development, implementation, and evaluation of a positive youth development program that attempts to promote the mental health of stressful Chinese adolescents using principles of Problem Solving Therapy (PST). There are two general aims of PST: to help clients identify life difficulties and resolve them, as well as to teach them skills on how to deal with future problems. The proposed project will utilize the principles of PST as the guiding framework to run two mental health promotion courses for adolescents who are experiencing disturbing stressful responses and students who want to improve their stress management style. Both objective and subjective outcome evaluation strategies will be carried out to assess the effectiveness of the intervention to promote the psychological well-being in adolescents who are experiencing stress. A related sample proposal is described that can give social workers some insight on how to prepare a proposal for developing the Tier 2 Program of the Project P.A.T.H.S. (Positive Adolescent Training through Holistic Social Programs).
Novel Therapeutic Approaches to the Treatment of Chronic Abdominal Visceral Pain
Authors: Patrizi Franca , Freedman Steven D. , Pascual-Leone Alvaro , Fregni Felipe
Published: May 2007

Chronic abdominal visceral pain (CAVP) has a significant clinical impact and represents one of the most frequent and debilitating disorders in the general population. It also leads to a significant economic burden due to workdays lost, reduced productivity, and long-term use of medications with their associated side effects. Despite the availability of several therapeutic options, the management of patients with CAVP is often inadequate, resulting in frustration for both patients and physicians. This may in part be explained by the lack of understanding of the mechanisms underlying chronic pain; in contrast with acute pain in which the pathophysiology is relatively well known and has several satisfactory therapeutic options. Recently, the development of tools for brain investigation, such as functional magnetic resonance imaging, has provided new insights on the pathophysiology of chronic pain. These new data have shown that plastic changes in the central and peripheral nervous system might play an important role in the maintenance of chronic pain. Therefore, approaches aimed at the modulation of the nervous system, rather than the ones interfering with the inflammatory pathways, may be more effective for chronic pain treatment. We propose that noninvasive central nervous system stimulation, with transcranial magnetic stimulation (TMS), might be a novel therapeutic option for CAVP. This paper will present an overview of the pathophysiology and the available therapies for CAVP, focusing on the recent advances in the treatment of this pathology.
Digital Three-Dimensional Atlas of Quail Development Using High-Resolution MRI
Authors: Ruffins Seth W. , Martin Melanie , Keough Lindsey , Truong Salina , Fraser Scott E. , Jacobs Russell E. , Lansford Rusty
Published: May 2007

We present an archetypal set of three-dimensional digital atlases of the quail embryo based on microscopic magnetic resonance imaging (μMRI). The atlases are composed of three modules: (1) images of fixedex ovoquail, ranging in age from embryonic day 5 to 10 (e05 to e10); (2) a coarsely delineated anatomical atlas of the μMRI data; and (3) an organ system-based hierarchical graph linked to the anatomical delineations. The atlas is designed to be accessed using SHIVA, a free Java application. The atlas is extensible and can contain other types of information including anatomical, physiological, and functional descriptors. It can also be linked to online resources and references. This digital atlas provides a framework to place various data types, such as gene expression and cell migration data, within the normal three-dimensional anatomy of the developing quail embryo. This provides a method for the analysis and examination of the spatial relationships among the different types of information within the context of the entire embryo.
Parameterization of the Stomatal Component of the DO3SE Model for Mediterranean Evergreen Broadleaf Species
Authors: Alonso Roccío , Elvira Susana , Sanz María J. , Emberson Lisa , Gimeno Benjamín S.
Published: May 2007

An ozone (O3) deposition model (DO3SE) is currently used in Europe to define the areas where O3concentrations lead to absorbed O3doses that exceed the flux-based critical levels above which phytotoxic effects would be likely recorded. This mapping exercise relies mostly on the accurate estimation of O3flux through plant stomata. However, the present parameterization of the modulation of stomatal conductance (gs) behavior by different environmental variables needs further adjustment if O3phytotoxicity is to be assessed accurately at regional or continental scales. A new parameterization of the model is proposed for Holm oak (Quercus ilex), a tree species that has been selected as a surrogate for all Mediterranean evergreen broadleaf species. This parameterization was based on a literature review, and was calibrated and validated using experimentally measured data of gsand several atmospheric and soil parameters recorded at three sites of the Iberian Peninsula experiencing long summer drought, and very cold and dry winter air (El Pardo and Miraflores) or milder conditions (Tietar). A fairly good agreement was found between modeled and measured data (R2= 0.64) at Tietar. However, a reasonable performance (R2= 0.47–0.62) of the model was only achieved at the most continental sites when gsand soil moisture deficit relationships were considered. The influence of root depth on gsestimation is discussed and recommendations are made to build up separate parameterizations for continental and marine-influenced Holm oak sites in the future.
Subjective Outcome Evaluation of the Project P.A.T.H.S.: Findings Based on the Perspective of the Program Participants
Authors: Shek Daniel T. L. , Ma Hing Keung
Published: May 2007

A total of 52 schools (n = 8679 students) participated in the experimental implementation phase of the project P.A.T.H.S. (Positive Adolescent Training through Holistic Social Programmes). After completion of the Tier 1 Program, students were invited to respond to the Subjective Outcome Evaluation Form (Form A) to assess their views of the program, instructors, and perceived effectiveness of the program. Based on the consolidated reports submitted by the schools to the funding body, the research team aggregated the consolidated data to form a “reconstructed” overall profile on the perceptions of the program participants. Results showed that high proportions of the respondents had positive perceptions of the program and the instructors, and roughly four-fifths of the respondents regarded the program as helpful to them. The present study provides additional support for the effectiveness of the Tier 1 Program of the P.A.T.H.S. Project in Hong Kong.
Intestinal Ileus as a Possible Cause of Hypobicarbonatemia
Authors: Serrano Andres , Chilakapati Rajani K. , Ghanayem Alexander J. , Yuan Yemin , Alberts Jeffery , Stephen Cathy , Rombola Giuseppe , Batlle Daniel
Published: May 2007

The possible occurrence of metabolic acidosis in patients with intestinal ileus is not well recognized. We describe a patient with acute alcohol-induced pancreatitis and a large transverse colon ileus in which plasma bicarbonate dropped rapidly in the absence of an increase in the plasma anion gap. The urinary anion gap and ammonium excretion were consistent with an appropriate renal response to metabolic acidosis and against the possibility of respiratory alkalosis. The cause of the falling plasma bicarbonate was ascribed to intestinal bicarbonate sequestration owing to the enhancement of chloride-bicarbonate exchange in a dilated paralyzed colon.
Impacts of Elevated Atmospheric CO2and O3on Paper Birch (Betula papyrifera): Reproductive Fitness
Authors: Darbah Joseph N. T. , Kubiske Mark E. , Nelson Neil , Oksanen Elina , Vaapavuori Elina , Karnosky David F.
Published: May 2007

Atmospheric CO2and tropospheric O3are rising in many regions of the world. Little is known about how these two commonly co-occurring gases will affect reproductive fitness of important forest tree species. Here, we report on the long-term effects of CO3and O3for paper birch seedlings exposed for nearly their entire life history at the Aspen FACE (Free Air Carbon Dioxide Enrichment) site in Rhinelander, WI. Elevated CO2increased both male and female flower production, while elevated O3increased female flower production compared to trees in control rings. Interestingly, very little flowering has yet occurred in combined treatment. Elevated CO2had significant positive effect on birch catkin size, weight, and germination success rate (elevated CO2increased germination rate of birch by 110% compared to ambient CO2concentrations, decreased seedling mortality by 73%, increased seed weight by 17%, increased root length by 59%, and root-to-shoot ratio was significantly decreased, all at 3 weeks after germination), while the opposite was true of elevated O3(elevated O3decreased the germination rate of birch by 62%, decreased seed weight by 25%, and increased root length by 15%). Under elevated CO2, plant dry mass increased by 9 and 78% at the end of 3 and 14 weeks, respectively. Also, the root and shoot lengths, as well as the biomass of the seedlings, were increased for seeds produced under elevated CO2, while the reverse was true for seedlings from seeds produced under the elevated O3. Similar trends in treatment differences were observed in seed characteristics, germination, and seedling development for seeds collected in both 2004 and 2005. Our results suggest that elevated CO2and O3can dramatically affect flowering, seed production, and seed quality of paper birch, affecting reproductive fitness of this species.
Subjective Outcome Evaluation of the Project P.A.T.H.S.: Findings Based on the Perspective of the Program Implementers
Authors: Shek Daniel T. L. , Siu Andrew M. H. , Lee Tak Yan
Published: May 2007

A total of 52 schools (n = 8679 students) participated in the experimental implementation phase of the Project P.A.T.H.S. (Positive Adolescent Training through Holistic Social Programmes). After completion of the Tier 1 Program, 344 instructors completed the Subjective Outcome Evaluation Form (Form B) to assess their views of the program, instructors, and perceived effectiveness of the program. Based on the consolidated reports submitted by the schools to the funding body, the research team aggregated the consolidated data to form a “reconstructed” overall profile on the perceptions of the program implementers. Results showed that high proportions of the workers had positive perceptions of the program and their own performance, and roughly 90% of the workers regarded the program as helpful to the program participants. The present study provides additional support for the effectiveness of the Tier 1 Program of the P.A.T.H.S. Project in Hong Kong.
Neurodegeneration in Schizophrenia: Evidence fromIn VivoNeuroimaging Studies
Authors: Csernansky John G.
Published: May 2007

Although schizophrenia is primarily considered to be a neurodevelopmental disorder, there is a growing consensus that the disorder may also involve neurodegeneration. Recent research using non-invasive neuroimaging techniques, such as magnetic resonance imaging, suggests that some patients with schizophrenia show progressive losses of gray matter in the frontal and temporal lobes of the brain. The cellular mechanisms responsible for such gray matter losses are unknown, but have been hypothesized to involve abnormal increases in apoptosis.
F-Waves – Physiology and Clinical Uses
Authors: Fisher Morris A.
Published: May 2007

F-waves are low amplitude responses produced by antidromic activation of motoneurons. They may not appear after each stimulus and are inherently variable in latency, amplitude, and configuration. Meaningful analysis of F-waves requires an appreciation of these characteristics of F-waves as well as an understanding of their physiology. These features of F-waves as well as their physiology are reviewed. This is important since F-waves are one of the most frequently used studies in clinical neurophysiology and much of the controversies surrounding the use of F-waves relates to a failure to adequately consider the requirements of F-wave analysis. These requirements include the number of F-waves that need to be recorded, the parameters that should be evaluated, and the muscle from which the F-waves are recorded. If analyzed correctly, current reports would indicate that F-waves are the most sensitive and reliable nerve conduction study for evaluating polyneuropathies, can be abnormal in focal proximal nerve dysfunction, can be at least as sensitive as needle electromyography for defining lumbosacral radiculopathies, and can provide a meaningful physiological window into disorders of the central nervous system. Reports supporting these statements and their clinical relevance are discussed.
Physician Satisfaction in a Cancer Prevention Program for Low-Income Women in Nevada
Authors: Cochran Christopher R. , Gorospe Emmanuel C.
Published: May 2007

Physicians and health care organizations that provide services to low-income patients are valuable partners in improving health care access for the uninsured and medically underserved. In this pilot study, we explored physicians' needs and factors for satisfaction in the Women's Health Connection (WHC), a breast and cervical cancer-screening program for low-income women in Nevada. Of the 126 physicians in the WHC program, 50 physicians completed a needs-and-satisfaction questionnaire. Survey data were subjected to factor analysis using Varimax rotation. The results yielded three components, which accounted for 65% of the variance. The three components or dimensions for physician satisfaction were: (1) appropriate administrative support and documentation, (2) availability of support for medical management, and (3) timeliness of diagnostic reports. Amount of reimbursement was not a significant factor. The respondents serving in this cancer prevention program for low-income women were satisfied in their involvement in the program. Further attention should be given on the identified issues for satisfaction among physicians, which could lead to quality improvement and serve as a model for other programs that serve low-income patients in cancer prevention.
Linking Increasing Drought Stress to Scots Pine Mortality and Bark Beetle Infestations
Authors: Dobbertin Matthias , Wermelinger Beat , Bigler Christof , Bürgi Matthias , Carron Mathias , Forster Beat , Gimmi Urs , Rigling Andreas
Published: May 2007

In the dry Swiss Rhone Valley, Scots pine forests have experienced increased mortality in recent years. It has commonly been assumed that drought events and bark beetles fostered the decline, however, whether bark beetle outbreaks increased in recent years and whether they can be linked to drought stress or increasing temperature has never been studied.

In our study, we correlated time series of drought indices from long-term climate stations, 11-year mortality trends from a long-term research plot, and mortality probabilities modeled from tree rings (as an indicator of tree vitality) with documented occurrences of various bark beetle species and a buprestid beetle, using regional Forest Service reports from 1902 to 2003 and advisory cases of the Swiss Forest Protection Service (SFPS) from 1984 to 2005. We compared the historical findings with measured beetle emergence from a 4-year tree felling and breeding chamber experiment.

The documented beetle-related pine mortality cases increased dramatically in the 1990s, both in the forest reports and the advisory cases. The incidents of beetle-related pine mortality correlated positively with spring and summer temperature, and with the tree-ring-based mortality index, but not with the drought index. The number of advisory cases, on the other hand, correlated slightly with summer drought index and temperature, but very highly with tree-ringbased mortality index. The tree-ring-based mortality index and observed tree mortality increased in years following drought. This was confirmed by the beetle emergences from felled trees. Following dry summers, more than twice as many trees were colonized by beetles than following wet summers.

We conclude that increased temperatures in the Swiss Rhone Valley have likely weakened Scots pines and favored phloeophagous beetle population growth. Beetles contributed to the increased pine mortality following summer drought. Among the factors not addressed in this study, changed forest use may have also contributed to increased beetle populations and Scots pine mortality, whereas air pollution seems to be of lesser importance.
Convergence of Subjective Outcome and Objective Outcome Evaluation Findings: Insights Based on the Project P.A.T.H.S.
Authors: Shek Daniel T. L. , Lee Tak Yan , Siu Andrew M. H. , Ma Hing Keung
Published: May 2007

A total of 546 students participated in the Tier 1 Program of the P.A.T.H.S. Project responded to the Chinese Positive Youth Development Scale (CPYDS) at pretest and posttest and the Subjective Outcome Scale (SOS) at posttest. Result showed that the SOS was internally consistent. The SOS total scores were significantly related to measures of global satisfaction and the participants' degree of sharing with others, thus giving support to its construct validity. Factor analysis revealed that there were three dimensions of the scale and the related subscales were significantly correlated among themselves. Based on the significant relationships between the SOS measures of perceived program effectiveness and posttest CPYDS scores as well as changes in CPYDS scores, the present study revealed the convergence of subjective outcome evaluation findings and objective outcome evaluation findings in the P.A.T.H.S. Project.
Galactosaminoglycan Function and Oligosaccharide Structure Determination
Authors: Seidler Daniela G. , Peter-Katalinic Jasna , Zamfir Alina D.
Published: May 2007

This review will discuss the importance of sequencing long chondroitin sulfate and dermatan sulfate chains specifically derived from decorin. Decorin is a member of the small leucine-rich repeat proteoglycans and ubiquitously expressed primarily in the skin. Sequence information and diverse function of glycosaminoglycans is further influenced by variable expression through the core protein indicating the importance to analyse glycosaminoglycans from specific proteoglycans.
Calculation of Theoretical and Empirical Nutrient N Critical Loads in the Mixed Conifer Ecosystems of Southern California
Authors: Breiner Joan , Gimeno Benjamin S. , Fenn Mark
Published: May 2007

Edaphic, foliar, and hydrologic forest nutrient status indicators from 15 mixed conifer forest stands in the Sierra Nevada, San Gabriel Mountains, and San Bernardino National Forest were used to estimate empirical or theoretical critical loads (CL) for nitrogen (N) as a nutrient. Soil acidification response to N deposition was also evaluated. Robust empirical relationships were found relating N deposition to plant N uptake (N in foliage), N fertility (litter C/N ratio), and soil acidification. However, no consistent empirical CL were obtained when the thresholds for parameters indicative of N excess from other types of ecosystems were used. Similarly, the highest theoretical CL for nutrient N calculated using the simple mass balance steady state model (estimates ranging from 1.4–8.8 kg N/ha/year) was approximately two times lower than the empirical observations. Further research is needed to derive the thresholds for indicators associated with the impairment of these mixed conifer forests exposed to chronic N deposition within a Mediterranean climate. Further development or parameterization of models for the calculation of theoretical critical loads suitable for these ecosystems will also be an important aspect of future critical loads research.
Smog Nitrogen and the Rapid Acidification of Forest Soil, San Bernardino Mountains, Southern California
Authors: Wood Yvonne A. , Fenn Mark , Meixner Thomas , Shouse Peter J. , Breiner Joan , Allen Edith , Wu Laosheng
Published: May 2007

We report the rapid acidification of forest soils in the San Bernardino Mountains of southern California. After 30 years, soil to a depth of 25 cm has decreased from a pH (measured in 0.01 M CaCl2) of 4.8 to 3.1. At the 50-cm depth, it has changed from a pH of 4.8 to 4.2. We attribute this rapid change in soil reactivity to very high rates of anthropogenic atmospheric nitrogen (N) added to the soil surface (72 kg ha–1 year–1) from wet, dry, and fog deposition under a Mediterranean climate. Our research suggests that a soil textural discontinuity, related to a buried ancient landsurface, contributes to this rapid acidification by controlling the spatial and temporal movement of precipitation into the landsurface. As a result, the depth to which dissolved anthropogenic N as nitrate (NO3) is leached early in the winter wet season is limited to within the top ~130 cm of soil where it accumulates and increases soil acidity.