bluuebunny/crossref_metadata_2025 huggingface dataset:
Medium-Sized Cities Facing the Demographic Challenge in Spain’s Low-Density Regions through Citizen Participation Projects
Authors: Rodríguez-Domenech María Ángeles
Published: April 2022

The roles of medium-sized cities in processes of demographic challenge have taken many different paths. New forms of urban sprawl, deconcentrating processes, and the emergence of the diffuse city have marked a change in the relations that Spanish medium-sized cities have traditionally had with their most directly influenced territories. In line with the theoretical framework of the European next generation urban regeneration programme, the main aim of this paper is to propose a methodology to develop a project that fosters resilience strategies and the revitalization of local environments. This will also benefit the institutions that are involved in promoting it. The innovative methodology employed has been denominated the “We Propose!” project and has received several national acknowledgments. This is a strategically designed civic participation urban renewal project and has been subject to geographical analysis through field trips and in situ research. A case study into urban renewal strategies was carried out in Ciudad Real, which is a medium-sized city in Spain’s third largest region. It includes an evaluation of both the design and implementation of what could be considered a successful case of urban renewal carried out in the city. This urban development initiative was undertaken by the public administration, but it was designed and proposed by local citizens.
Evaluation of a Novel Tool for Apical Plug Formation during Apexification of Immature Teeth
Authors: Tolibah Yasser Alsayed , Droubi Line , Alkurdi Saleh , Abbara Mohammad Tamer , Bshara Nada , Lazkani Thuraya , Kouchaji Chaza , Ahmad Ibrahim Ali , Baghdadi Ziad D.
Published: April 2022

The purpose of this study was to compare the sealing ability and time required for the formation of Biodentine and mineral trioxide aggregation (MTA) apical plugs, using three different delivery methods: an amalgam carrier (AC), the Micro Apical Placement (MAP) System or a novel tool using a modified cannula (MC). Materials and Methods: A total of 60 uniformed molar roots were divided into three main groups, according to the technique of apical plug formation: AC, MAP, and MC. Each group was divided into two subgroups, according to the filling material used: MTA and Biodentine. A timer was used to calculate the required time for apical plug formation. After setting the filling materials, the apical microleakage of the formed plugs was quantified using the dye extraction method and spectrophotometry. The differences between the groups were analyzed using the one-way ANOVA and LSD post hoc tests. The significance level was set at 0.05. Results: No significant differences were reported in the time required to form the apical plugs in all groups (p > 0.05). However, the apical plugs formed by the AC method had significantly higher microleakage than those formed using the MAP and MC methods (p < 0.05). Conclusion: Within the limitations of this study, the sealing ability of the apical plugs formed by the MC method is comparable to the MAP method and better than the AC method.
Type of Findings Generated by the Occupational Therapy Workforce Research Worldwide: Scoping Review and Content Analysis
Authors: Jesus Tiago S. , Mani Karthik , von Zweck Claudia , Kamalakannan Sureshkumar , Bhattacharjya Sutanuka , Ledgerd Ritchard ,   on behalf of the World Federation of Occupational Therapists
Published: April 2022

Occupational therapists are needed to meet the health and occupational needs of the global population, but we know little about the type of findings generated by occupational therapy workforce research conducted worldwide. We aim to synthesize these findings and their range of content to inform future investigations. A scoping review with content analysis was used. Six scientific databases, websites of official institutions, snowballing, and key informants were used for searches. Two independent reviewers took selection decisions against the eligibility criteria published a priori in the review protocol. Of the 1246 unique references detected, 57 papers were included for the last 25 years. A total of 18 papers addressed issues of attractiveness and retention, often in Australia, and 14 addressed the issues of supply, demand, and distribution, often in the US. Only these two categories generated subtopics. Many workforce issues were rarely addressed as a main topic (e.g., race/ethnic representation). Cross-national, cross-regional, or cross-professional studies generated more actionable findings. Overall, we found few discernable trends, minimal evidence of research programs, and various gaps in content coverage or in the use of contemporary research approaches. There is a need for a coordinated strengthening of the occupational therapy workforce research worldwide.
Sampling Inspection Plan to Test Daily COVID-19 Cases Using Gamma Distribution under Indeterminacy Based on Multiple Dependent Scheme
Authors: Aslam Muhammad , Rao Gadde Srinivasa , Albassam Mohammed
Published: April 2022

The purpose of this paper is to develop a multiple dependent state (MDS) sampling plan based on time-truncated sampling schemes for the daily number of cases of the coronavirus disease COVID-19 using gamma distribution under indeterminacy. The proposed sampling scheme parameters include average sample number (ASN) and accept and reject sample numbers when the indeterminacy parameter is known. In addition to the parameters of the proposed sampling schemes, the resultant tables are provided for different known indeterminacy parametric values. The outcomes resulting from various sampling schemes show that the ASN decreases as indeterminacy values increase. This shows that the indeterminacy parameter plays a vital role for the ASN. A comparative study between the proposed sampling schemes and existing sampling schemes based on indeterminacy is also discussed. The projected sampling scheme is illustrated with the help of the daily number of cases of COVID-19 data. From the results and real example, we conclude that the proposed MDS sampling scheme under indeterminacy requires a smaller sample size compared to the single sampling plan (SSP) and the existing MDS sampling plan.
Environmental Justice in Greater Los Angeles: Impacts of Spatial and Ethnic Factors on Residents’ Socioeconomic and Health Status
Authors: Jiang Yuliang , Yang Yufeng
Published: April 2022

Environmental justice advocates that all people are protected from disproportionate impacts of environmental hazards. Despite this ideal aspiration, social and environmental inequalities exist throughout greater Los Angeles. Previous research has identified and mapped pollutant levels, demographic information, and the population’s socioeconomic status and health issues. Nevertheless, the complex interrelationships between these factors remain unclear. To close this knowledge gap, we first measured the spatial centrality using sDNA software. These data were then integrated with other socioeconomic and health data collected from CalEnvironScreen, with census tract as the unit of analysis. Finally, structural equation modeling (SEM) was executed to explore direct, indirect, and total effects among variables. The results show that the White population tends to reside in the more segregated areas and lives closer to green space, contributing to higher housing stability, financial security, and more education attainment. In contrast, people of color, especially Latinx, experience the opposite of the environmental benefits. Spatial centrality exhibits a significant indirect effect on environmental justice by influencing ethnicity composition and pollution levels. Moreover, green space accessibility significantly influences environmental justice via pollution. These findings can assist decision-makers to create a more inclusive society and curtail social segregation for all individuals.
Quantitative Methods to Detect Suicide and Self-Harm Clusters: A Systematic Review
Authors: Benson Ruth , Rigby Jan , Brunsdon Christopher , Cully Grace , Too Lay San , Arensman Ella
Published: April 2022

Suicide and self-harm clusters exist in various forms, including point, mass, and echo clusters. The early identification of clusters is important to mitigate contagion and allocate timely interventions. A systematic review was conducted to synthesize existing evidence of quantitative analyses of suicide and self-harm clusters. Electronic databases including Medline, Embase, Web of Science, and Scopus were searched from date of inception to December 2020 for studies that statistically analyzed the presence of suicide or self-harm clusters. Extracted data were narratively synthesized due to heterogeneity among the statistical methods applied. Of 7268 identified studies, 79 were eligible for narrative synthesis. Most studies quantitatively verified the presence of suicide and self-harm clusters based on the scale of the data and type of cluster. A Poisson-based scan statistical model was found to be effective in accurately detecting point and echo clusters. Mass clusters are typically detected by a time-series regression model, although limitations exist. Recently, the statistical analysis of suicide and self-harm clusters has progressed due to advances in quantitative methods and geospatial analytical techniques, most notably spatial scanning software. The application of such techniques to real-time surveillance data could effectively detect emerging clusters and provide timely intervention.
Validation of the Spanish Version of the Double Knowledge Expectations and Received Knowledge Significant Other Scale for Informal Caregivers of People with Dementia (KESO-DEM/RKSO-DEM)
Authors: Frías Cindy E. , Casafont Claudia , Cabrera Esther , Zabalegui Adelaida
Published: April 2022

Dementia is associated with cognitive decline. Becoming an informal caregiver raises questions, requiring information and support from health professionals to guide home care. A multicenter, longitudinal study was carried out to validate the Spanish version of the double scale of expected and received knowledge for informal caregivers of people with dementia (KESO-DEM/RKSO-DEM), the analysis of the dimensional structure of the instrument, its validity and reliability, and temporary stability was carried out. An analysis of criterion and construct validity, internal consistency, and test–retest stability was performed. The evaluation of the interrelation between dimensions was statistically significant. Regarding internal consistency, the scale values were good both for the scale totals and for each dimension of knowledge, with Cronbach’s alpha coefficients of 0.97. For criterion validity, all items showed temporal stability for both questionnaires (p < 0.05). The availability of a valid, reliable tool for the measurement of expected and received knowledge in caregivers of people with dementia allows an approach based on the real needs of the family and the patient. It is important to design care protocols for people with dementia that are adapted to their needs and expectations and to their non-curative treatment, to improve the emotional well-being of patients and informal caregivers.
The Magnitude of NCD Risk Factors in Ethiopia: Meta-Analysis and Systematic Review of Evidence
Authors: Tesfay Fisaha Haile , Backholer Kathryn , Zorbas Christina , Bowe Steven J. , Alston Laura , Bennett Catherine M.
Published: April 2022

Background: Non-communicable Diseases (NCDs) and their risk factors are the leading contributors to morbidity and mortality globally, particularly in low- and middle-income countries including Ethiopia. To date, there has been no synthesis of the literature on the relative prevalence of NCD risk factors in Ethiopia. Methodology: We conducted a systematic review and meta-analysis of primary studies reporting on the prevalence of NCD risk factors in Ethiopia published in English from 2012 to July 2020. Pre-tested NCD search terms were applied to Medline, Embase, Scopus, CINAHL, and Global Health. Three reviewers screened and appraised the quality of the identified papers. Data extraction was conducted using a pilot tested proforma. Meta-analysis was conducted using Stata 16 and pooled prevalence estimated with associated 95% confidence intervals. Clinically heterogeneous studies that did not fulfil the eligibility criteria for meta-analysis were narratively synthesised. I2 was used to assess statistical heterogeneity. Results: 47 studies fulfilled the inclusion criteria and contributed 68 NCD risk factor prevalence estimates. Hypertension was the most frequently examined NCD risk factor, with a pooled prevalence of 21% (n = 27 studies). The pooled prevalence percentages for overweight and obesity were 19.2% and 10.3%, respectively (n = 7 studies each), with a combined prevalence of 26.8% (n = 1 study). It was not possible to pool the prevalence of alcohol consumption, smoking, metabolic disorders, or fruit consumption because of heterogeneity across studies. The prevalence of alcohol use, as reported from the included individual studies, ranged from 12.4% to 13.5% (n = 7 studies). More than 90% of participants met the WHO-recommended level of physical activity (n = 5 studies). The prevalence of smoking was highly variable, ranging between 0.8% and 38.6%, as was the prevalence of heavy alcohol drinking (12.4% to 21.1%, n = 6 studies) and metabolic syndrome (4.8% to 9.6%, n = 5 studies). Fruit consumption ranged from 1.5% up to the recommended level, but varied across geographic areas (n = 3 studies). Conclusion and recommendations: The prevalence of NCD risk factors in Ethiopia is relatively high. National NCD risk factor surveillance is required to inform the prioritisation of policies and interventions to reduce the NCD burden in Ethiopia.
Effects of Montmorency Tart Cherry and Blueberry Juice on Cardiometabolic and Other Health-Related Outcomes: A Three-Arm Placebo Randomized Controlled Trial
Authors: Sinclair Jonathan , Bottoms Lindsay , Dillon Stephanie , Allan Robert , Shadwell Gareth , Butters Bobbie
Published: April 2022

The current study aimed to investigate the influence of tart cherry and blueberry juices on cardiometabolic and other health indices following a 20-day supplementation period. Forty-five adults were randomly assigned to receive tart cherry, blueberry, or a placebo, of which they drank 60 mL per day for 20 days. The primary outcome, which was systolic blood pressure, and secondary measures, including anthropometric, energy expenditure, substrate oxidation, hematological, diastolic blood pressure/resting heart rate, psychological wellbeing, and sleep efficacy, were measured before and after the intervention. There were no statistically significant differences (p > 0.05) for systolic blood pressure; however, total and LDL cholesterol were significantly improved with blueberry intake (pre: total cholesterol = 4.36 mmol/L and LDL cholesterol = 2.71 mmol/L; post: total cholesterol = 3.79 mmol/L and LDL cholesterol = 2.23 mmol/L) compared to placebo (pre: total cholesterol = 4.01 mmol/L and LDL cholesterol = 2.45 mmol/L; post: total cholesterol = 4.34 mmol/L and LDL cholesterol = 2.67 mmol/L). Furthermore, psychological wellbeing indices measured using the Beck Depression Inventory, State Trait Anxiety Inventory, and COOP WONCA improved statistically in the blueberry arm compared to placebo. Given the clear association between lipid concentrations and the risk of cardiovascular disease as well as the importance of psychological wellbeing to health-related quality of life, this investigation indicates that it could be an effective approach to assist in managing cardiometabolic disease.
Mis–Dis Information in COVID-19 Health Crisis: A Narrative Review
Authors: Clemente-Suárez Vicente Javier , Navarro-Jiménez Eduardo , Simón-Sanjurjo Juan Antonio , Beltran-Velasco Ana Isabel , Laborde-Cárdenas Carmen Cecilia , Benitez-Agudelo Juan Camilo , Bustamante-Sánchez Álvaro , Tornero-Aguilera José Francisco
Published: April 2022

Background: In this narrative review, we address the COVID-19 pandemic mis–dis information crisis in which healthcare systems have been pushed to their limits, with collapses occurring worldwide. The context of uncertainty has resulted in skepticism, confusion, and general malaise among the population. Informing the public has been one of the major challenges during this pandemic. Misinformation is defined as false information shared by people who have no intention of misleading others. Disinformation is defined as false information deliberately created and disseminated with malicious intentions. Objective: To reach a consensus and critical review about mis–dis information in COVID-19 crisis. Methods: A database search was conducted in PsychINFO, MedLine (Pubmed), Cochrane (Wiley), Embase and CinAhl. Databases used the MeSH-compliant keywords of COVID-19, 2019-nCoV, Coronavirus 2019, SARS-CoV-2, misinformation, disinformation, information, vaccines, vaccination, origin, target, spread, communication. Results: Both misinformation and disinformation can affect the population’s confidence in vaccines (development, safety, and efficacy of vaccines, as well as denial of the severity of SARS-CoV infection). Institutions should take into account that a great part of the success of the intervention to combat a pandemic has a relationship with the power to stop the misinformation and disinformation processes. The response should be well-structured and addressed from different key points: central level and community level, with official and centralized communication channels. The approach should be multifactorial and enhanced by the collaboration of social media companies to stop misleading information, and trustworthy people both working or not working in the health care systems to boost the power of the message. Conclusions: The response should be well-structured and addressed from different key points: central level and community level, with official and clearly centralized communication channels. The approach should be multifactorial and enhanced from the collaboration of social media companies to stop misleading information, and trustworthy people both working and not working in the health care systems to boost the power of a message based on scientific evidence.
Assessing Urban Policies in a COVID-19 World
Authors: Śleszyński Przemysław , Legutko-Kobus Paulina , Rosenberg Mark , Pantyley Viktoriya , Nowak Maciej J.
Published: April 2022

The aim of this study was to identify how the literature analyzes (identifies, evaluates, forecasts, etc.) the relationship between health issues and urban policy in relation to the COVID-19 pandemic. Four main levels were identified in these cases: (1) direct demands for changes in health care, (2) social issues, (3) spatial organization and (4) redefining the tasks of public authority in the face of identified challenges. The basic working method used in the study assumed a critical analysis of the literature on the subject. The time scope of the search covered articles from January 2020 to the end of August 2021 (thus covering the period of three pandemic waves). Combinations of keywords in the titles were used to search for articles. The health perspective pointed to the need for urban policies to develop a balance between health and economic costs and for coordination between different professionals/areas. A prerequisite for such a balance in cities is the carrying out of social and spatial analyses. These should illustrate the diversity of the social situations in individual cities (and more broadly in urban areas, including, sometimes, large suburbs) and the diversity’s relationship (both in terms of causes and consequences) to the severity of pandemics and other health threats.
Development and Psychometric Evaluation of the Chinese Version of the Life Skills Scale for Physical Education
Authors: Ji Xiangbo , Zheng Shaofeng , Cheng Chuanyin , Cheng Liping , Cronin Lorcan
Published: April 2022

Research on life skills in physical education (PE) has gained great attention in recent years. However, there is a need to translate life skills measures for PE into other languages. This research adapted the Life Skills Scale for PE (LSSPE) into Chinese and provided evidence for its validity and reliability. In Study 1, the scale was cross-culturally adapted through translation and back-translation, expert feedback, pilot testing, and scale refinement to provide evidence for the content validity of the scale. Study 2 provided evidence for the factorial validity, internal consistency reliability, and test–retest reliability of the scale by testing it with 583 students. Study 3, with 390 students, provided evidence for the nomological validity of the measure, with results showing perceived teacher autonomy support and students’ basic need satisfaction were positively associated with life skills development in PE, and that controlling teaching and basic need frustration were negatively associated with life skills development. In conclusion, the results illustrate that the LSSPE can be used to evaluate Chinese-speaking students’ life skills development in PE.
Compliance with Standard Precaution and Its Relationship with Views on Infection Control and Prevention Policy among Chinese University Students during the COVID-19 Pandemic
Authors: Cheng Winnie Lai Sheung , Kwong Enid Wai Yung , Lee Regina Lai Tong , Tang Anson Chui Yan , Wong Lokki Lok Ki
Published: April 2022

Background: COVID-19 has placed tremendous pressure on the global public health system and has changed daily life. Aim: To examine the relationships between the perceived threat, perceived stress, coping responses and infection control practices towards the COVID-19 pandemic among university students in China. Methods: Using a cross-sectional survey, 4392 students were recruited from six universities in two regions of China. Methods: Data were collected via an online platform using self-reported questionnaires. Hierarchical multiple regression analyses were performed to predict the variables on COVID-19 infection control practices. Results: Pearson correlation coefficients showed a significant negative relationship between perceived stress and COVID-19 infection control practices. A significant positive relationship was observed between wishful thinking and empathetic responding, and infection control practices. Hierarchical multiple regression analyses revealed that gender, geographical location, perceived stress and emotion-focused and relationship-focused coping responses were predictors of COVID-19 infection control practices. Conclusions: The findings suggest that university students displayed moderate levels of stress, using wishful thinking and empathetic responses as coping strategies. Counselling services should therefore emphasise reassurance and empathy. Male university students tended to be less compliant with social distancing. Both counselling and public health measures should recognise the importance of gender differences. Nurses should integrate these findings into future health programme planning and interventions.
Steam-Exploded Pruning Waste as Peat Substitute: Physiochemical Properties, Phytotoxicity and Their Implications for Plant Cultivation
Authors: Yang Rui , Chen Xuejiao , Zhang Dongdong , Wang Hong , Zhou Wanlai , Lin Wei , Qi Zhiyong
Published: April 2022

Peat is a nonrenewable resource that we are using at alarming rates. Development of peat alternative from pruning waste (PW) could be a cost- and environment-friendly way of disposal. Steam explosion (SE) is a commonly used pretreatment of lignocellulosic biomass, but its impact on the properties of PW as a growing substrate is largely unknown. To address this issue, PW was treated using five SE temperatures (160, 175, 190, 205 and 220 °C) and three retention times (1, 3 and 5 min) and evaluated for key traits of growing substrate. Results indicate that bulk density, total porosity, EC, total carbon, and concentration of phytotoxins including phenol, flavonoid, and alkaloid significantly increased or tended to increase with increasing temperature and/or retention time. A reversed trend was observed for water-holding capacity, pH, content of hemicellulose and lignin, and germination index. Cation exchange capacity and total N showed minimal response to SE. Steam explosion had inconsistent impacts on acid soluble nutrients. Phytotoxicity was a major factor limiting the use of SE-treated PW as growing substrate. Higher pretreatment severity led to higher phytotoxicity but also facilitated subsequent phytotoxicity removal by torrefaction. Pruning waste treated by SE and torrefaction under certain conditions may be used as peat substitute for up to 40% (v/v).
Insights into Improving Risk and Safety Communication through Environmental Health Literacy
Authors: Lindsey Marti , Richmond Ben , Quintanar Daniel R. , Spradlin Jordan , Halili Loren
Published: April 2022

Messages and materials developed to communicate risk to the public are often misunderstood because the public misperceives risk, science information is too complex, leading to audience misunderstandings, and an overarching focus on the details of the problem without supplying solutions or actions to keep the public safe. This article describes the creation of a communication model to improve risk communication that includes safety information. The authors describe essential components of Risk and Safety Communication based on features of Environmental Health Literacy (EHL), which informed the creation of a protocol for developing risk communication messages and materials. An online training module was developed to aid communicators in creating information to enable the public to protect themselves, their family, and their community, leading to improved comprehension of how the environment impacts health. These principles were developed in a series of focus groups, identifying how the public perceives risk, how they prefer to receive communication, and how participants respond to materials developed using the principles. Important topics discussed are understanding the literacy levels of the target audience, applying that understanding to developing messages, how risk perception leads to misperceptions and how to address those misperceptions by using plain language when developing focused messages and materials.
Frequency of Breakfast Eating and Obesity Prevalence in Primary School Teachers
Authors: Uvacsek Martina , Simkó Georgina , Boda-Ujlaky Judit , Kneffel Zsuzsanna
Published: April 2022

There is a lack of research using objective measures about teachers’ physical characteristics and lifestyle. The purpose of the study was to evaluate the relationship between frequency of breakfast consumption and body size, body composition, blood pressure and lifestyle factors in teachers from Hungary. The study analyzed data collected from 99 female primary school teachers (50.6 ± 6.6 year) before the COVID-19 pandemic. Anthropometric and resting blood pressure measurements were taken for each participant. Questionnaires were used to assess lifestyle factors (i.e., physical activity level, smoking status and eating breakfast). The frequency of eating breakfast was classified as rarely or never (0–2 days), some days (3–5 days) and regularly (6–7 days). Sixty-five percent of female teachers consumed breakfast 6–7 days/week, and this regular eating habit was positively associated with a lower risk of obesity (OR 0.29; CI: 0.10–0.80). In our sample, a higher frequency of breakfast consumption was not significantly associated with smoking (OR 2.65; CI: 0.17–1.37), hypertension (OR 0.61; CI: 0.23–1.65) and inactivity (OR 2.80; CI: 0.26–1.84). A beneficial effect of eating breakfast regularly was found on body composition in female teachers. Further studies should focus on the health behaviors of teachers and their relationship with physical activity or diagnosed diseases in urban and rural areas.
Self-Reported Medication Use among Pregnant and Postpartum Women during the Third Wave of the COVID-19 Pandemic: A European Multinational Cross-Sectional Study
Authors: Gerbier Eva , Favre Guillaume , Tauqeer Fatima , Winterfeld Ursula , Stojanov Milos , Oliver Alison , Passier Anneke , Nordeng Hedvig , Pomar Léo , Baud David , Panchaud Alice , Meyer-Massetti Carla , Ceulemans Michael
Published: April 2022

Information on medication utilization among pregnant and postpartum women during the pandemic is lacking. We described the prevalence and patterns of self-reported medication use among pregnant and postpartum women during the third wave of the pandemic (June–August 2021). An online questionnaire was distributed in five European countries between June–August 2021. Pregnant women or women who had delivered in the three preceding months, and ≥18 years old, could participate. The prevalence of overall medication use, self-medication, and changes in chronic medication use were determined. A total of 2158 women out of 5210 participants (41.4%) used at least one medication. Analgesics (paracetamol), systemic antihistamines (cetirizine), and drugs for gastric disorders (omeprazole) were the three most used classes. Anti-infectives were less prevalent than during pre-pandemic times. Antidepressants and anxiety related medication use remained similar, despite a higher prevalence of these symptoms. Self-medication was reported in 19.4% of women, and 4.1% of chronic medication users reported that they changed a chronic medication on personal initiative due to the pandemic. In conclusion, medication use patterns in our cohort were mostly similar to those of the first COVID-19 wave and the pre-pandemic period. More studies are needed to explore factors associated with self-medication and changes in chronic medication use due to the pandemic in this perinatal population.
Intermittent Hypoxia as a Therapeutic Tool to Improve Health Parameters in Older Adults
Authors: Timon Rafael , González-Custodio Adrián , Vasquez-Bonilla Aldo , Olcina Guillermo , Leal Alejo
Published: April 2022

Aging is associated with metabolic alterations, and with a loss of strength, muscle and bone mass. Moderate intermittent hypoxia has been proposed as a new tool to enhance health-related function. The aim of this study was to evaluate the effect of moderate intermittent hypoxia exposures on parameters related to cardiovascular and bone health in older adults. A total of 38 healthy older adults (aged 65–75 years) were divided into two groups: control group (C), and hypoxia group (H) that was subjected to an intermittent hypoxia exposure (at simulated altitude of 2500 m asl) during a 24-week period (3 days/week). Body composition, blood pressure, metabolic parameters (Cholesterol, triglycerides and glucose), C-reactive protein (CRP), vascular cell adhesion molecule-1 (VCAM-1), interleukin 8 (IL-8), interleukin 10 (IL-10), N-terminal propeptide of type I procollagen (PINP) and beta C-terminal telopeptide of collagen bone formation (b-CTX) were analyzed before and after the intervention. A repeated measures analysis of variance was performed to evaluate between-group differences. The results showed that the hypoxia group achieved after the intervention a decrease in fat mass, CRP (pro-inflammatory biomarker) and b-CTX (bone resorption biomarker), as well as an increase in PINP (bone formation biomarker). In conclusion, the intermittent hypoxia might be a useful therapeutic tool to deal with problems associated with aging, such as the increase in body fat, the loss of bone mass or low-grade inflammation.
Nexus between Climate-Smart Livestock Production Practices and Farmers’ Nutritional Security in Pakistan: Exploring Level, Linkages, and Determinants
Authors: Shahbaz Pomi , Abbas Azhar , Aziz Babar , Alotaibi Bader Alhafi , Traore Abou
Published: April 2022

Livestock plays a vital role in humans’ food and nutrition security under rapidly changing climatic scenarios. This study investigates the nature and factors affecting livestock farmers’ choices of climate-smart livestock practices by using a multivariate probit model and then estimates the average effect of these adopted strategies on per capita daily dietary (calorie, protein, and calcium) intake among livestock herders. For this purpose, data were collected from 196 livestock farmers residing in the Punjab province of Pakistan, selected through multistage purposive and random sampling. The Simpson diversity index results revealed that farmers used diversified food in their daily diet. The results also showed that farmers consumed more protein-rich food items as compared to calorie and calcium-rich food items in their daily diet. Moreover, the average per capita calorie intake of livestock farmers was 2413.19 kcal/day. Livestock farmers adopting a higher number of climate-smart livestock practices consumed more daily per capita calories, protein, and calcium compared to those who adopted a lower number of climate-smart livestock practices on livestock farms. Moreover, climate-smart livestock practices produced more and better nutritional outcomes in combination with each other than in isolation. Livestock training was found to be positively associated with the adoption of more climate-smart practices. Therefore, livestock training is necessary to expedite the adoption of climate-smart practices and to improve the nutritional security of the farmers.
Age at Arrival and Depression among Mexican Immigrant Women in Alabama: The Moderating Role of Culture
Authors: Andrews Courtney , Oths Kathryn , Dressler William
Published: April 2022

Mexican-born women in the U.S. are at high risk of depression. While acculturation is the primary analytical framework used to study immigrant mental health, this research suffers from (1) a lack of specificity regarding how cultural models of living and being take shape among migrants converging in new destinations in the U.S., and (2) methods to empirically capture the impact of cultural positioning on individual health outcomes. Instead of relying on proxy measures of age at arrival and time in the U.S. to indicate where an individual is located on the acculturation spectrum, this study uses cultural consensus analysis to derive the substance and structure of a cultural model for la buena vida (the good life) among Mexican immigrant women in Birmingham, Alabama, and then assesses the extent to which respondents are aligned with the model in their everyday lives. This measure of ‘cultural consonance’ is explored as a moderating variable between age at arrival in the U.S. and number of depressive symptoms. Results demonstrate that for those who arrived at an older age, those with lower consonance are at the highest risk for depression, while those who are more aligned with la buena vida are at lower risk.
Application of Phosphate Materials as Constructed Wetland Fillers for Efficient Removal of Heavy Metals from Wastewater
Authors: Wu Xiaodan , Hong Ni , Cen Qingjing , Lu Jiaxin , Wan Hui , Liu Wei , Zheng Hongli , Ruan Roger , Cobb Kirk , Liu Yuhuan
Published: April 2022

Constructed wetlands are an environmentally friendly and economically efficient sewage treatment technology. Heavy metals (HMs) removal is always regarded as one of the most important tasks in constructed wetlands, which have aroused increasing concern in the field of contamination control in recent times. The fillers of constructed wetlands play an important role in HMs removal. However, traditional wetland fillers (e.g., zeolite, sand, and gravel) are known to be imperfect because of their low adsorption capacity. Regarding HMs removal, our work involved the selection of prominent absorbents, the evaluation of adsorption stability for various treatments, and then the possibility of applying this HM removal technology to constructed wetlands. For this purpose, several phosphate materials were tested to remove the heavy metals Cu and Zn. Three good phosphates including hydroxyapatite (HAP), calcium phosphate (CP), and physic acid sodium salt hydrate (PAS) demonstrated fast removal efficiency of HMs (Cu2+, Zn2+) from aqueous solution. The maximum removal rates of Cu2+ and Zn2+ by HAP, CP, and PAS reached 81.6% and 95.8%; 66.9% and 70.4%; 98.8% and 1.99%, respectively. In addition, better adsorption stability of these heavy metals was found to occur with a wide variation of desorption time and pH range. The most remarkable efficiency for heavy metal removal among tested phosphates was PAS, followed by HAP and CP. This study can provide a basis for the application of HMs removal in manmade wetland systems.
Spatiotemporal Evolution of the Urban Thermal Environment Effect and Its Influencing Factors: A Case Study of Beijing, China
Authors: Ren Ziqi , Li Zhe , Wu Feng , Ma Huiqiang , Xu Zhanjun , Jiang Wei , Wang Shaohua , Yang Jun
Published: April 2022

Rapid urbanization has led to significant changes in land surface temperature (LST), which in turn affect the urban thermal environment effect and the health of residents. Exploring the causes of the urban thermal environment effect will provide guidance for promoting sustainable urban development. The spatiotemporal evolution of the urban thermal environment effect within the sixth ring road of Beijing was analyzed by inversion of remote sensing data to obtain the LST in 2004, 2009, 2014, and 2019. In addition, based on multivariate spatial data, we applied the standard deviation ellipse (SDE), spatial principal component analysis (PCA), and other methods to analyze and identify the relationships between the urban thermal environment effect and its influencing factors. The results show that from 2004 to 2019, the spatial distribution of urban development and LST within the sixth ring road of Beijing were closely related, the heat island area showed a small increasing trend, and differences in the thermal environment effect between different administrative regions in different periods were obvious. The main factors affecting the urban thermal environment effect were urban construction intensity, vegetation and water bodies, socioeconomic activities, and geomorphology. It is noteworthy that human factors had a greater impact than natural factors. Among them, the positive effect of the normalized difference impervious surface index (NDBBI) and the negative effect of the fractional vegetation cover (FVC) were the most prominent. This study provides theoretical support for mitigating the urban thermal environment effect and promoting sustainable urban development.
Geographic Approach: Identifying Relatively Stable Tibetan Dialect and Subdialect Area Boundaries
Authors: Duan Mingyuan , Zhou Shangyi
Published: April 2022

Updating dialect maps requires extensive language surveys. Geographic methods can be applied to identify relatively stable boundaries of dialect and subdialect areas, allowing language surveys to focus on boundaries that may change and thereby reduce survey costs. Certain scholars have pointed out that the watershed boundary can be employed as the boundary of Tibetan dialect areas. This paper adds that the lowest-grade road breakpoint line and no-man’s-land boundary can also be used as essential indicators for determining stable (sub)dialect area boundaries. Combined with the revised First Law of Geography and the method of superposition analysis of geographic elements, this study identifies indicators that affect the stability of the Tibetan (sub)dialect area boundaries and evaluates the stability of each boundary segment. Due to the particularity of the study area, most Qinghai–Tibet Plateau (Chinese part) (sub)dialect area boundaries are stable. In addition, boundary inaccuracies caused by defects in the distribution of language survey samples can be identified by geographic approaches.
Long Non-Coding LEF1-AS1 Sponge miR-5100 Regulates Apoptosis and Autophagy in Gastric Cancer Cells via the miR-5100/DEK/AMPK-mTOR Axis
Authors: Zhang Huimin , Wang Jun , Wang Yundan , Li Jiapeng , Zhao Lili , Zhang Tongcun , Liao Xinghua
Published: April 2022

DEK and miR-5100 play critical roles in many steps of cancer initiation and progression and are directly or indirectly regulated by most promoters and repressors. LEF1-AS1 as a long non-coding RNA can regulate tumor development through sponge miRNA. The effect and regulatory mechanism of DEK on autophagy and apoptosis in gastric cancer (GC), and the role between miR-5100 and DEK or miR-5100 and LEF1-AS1 are still unclear. Our study found that DEK was highly expressed in gastric cancer tissues and cell lines, and knockdown of DEK inhibited the autophagy of cells, promoted apoptosis, and suppressed the malignant phenotype of gastric cancer. DEK regulates autophagy and apoptosis through the AMPK/mTOR signaling pathway. In addition, miR-5100 inhibits autophagy and promotes apoptosis in GC cells while LEF1-AS1 had the opposite effect. Studies have shown that miR-5100 acts by targeting the 3′UTR of DEK, and LEF1-AS1 regulates the expression of miR-5100 by sponging with mIR-5100. In conclusion, our results found that LEF1-AS1 and miR-5100 sponge function, and the miR-5100/DEK/AMPK/mTOR axis regulates autophagy and apoptosis in gastric cancer cells.
Effects of Module Truncation of a New Alginate Lyase VxAly7C from Marine Vibrio xiamenensis QY104 on Biochemical Characteristics and Product Distribution
Authors: Tang Luyao , Bao Mengmeng , Wang Ying , Fu Zheng , Han Feng , Yu Wengong
Published: April 2022

Alginate lyase has received extensive attention as an important tool for oligosaccharide preparation, pharmaceutical production, and energy biotransformation. Noncatalytic module carbohydrate-binding modules (CBM) have a major impact on the function of alginate lyases. Although the effects of two different families of CBMs on enzyme characteristics have been reported, the effect of two combined CBM32s on enzyme function has not been elucidated. Herein, we cloned and expressed a new multimodular alginate lyase, VxAly7C, from Vibrioxiamenensis QY104, consisting of two CBM32s at N-terminus and a polysaccharide lyase family 7 (PL7) at C-terminus. To explore the function of CBM32s in VxAly7C, full-length (VxAly7C-FL) and two truncated mutants, VxAly7C-TM1 (with the first CBM32 deleted) and VxAly7C-TM2 (with both CBM32s deleted), were characterized. The catalytic efficiency of recombinant VxAly7C-TM2 was 1.82 and 4.25 times higher than that of VxAly7C-TM1 and VxAly7C-FL, respectively, indicating that CBM32s had an antagonistic effect. However, CBM32s improved the temperature stability, the adaptability in an alkaline environment, and the preference for polyG. Moreover, CBM32s contributed to the production of tri- and tetrasaccharides, significantly affecting the end-product distribution. This study advances the understanding of module function and provides a reference for broader enzymatic applications and further enzymatic improvement and assembly.
RNA-Seq Analysis Identifies Transcription Factors Involved in Anthocyanin Biosynthesis of ‘Red Zaosu’ Pear Peel and Functional Study of PpPIF8
Authors: Ma Zhenyu , Wei Chuangqi , Cheng Yudou , Shang Zhonglin , Guo Xiulin , Guan Junfeng
Published: April 2022

Red-skinned pears are favored by people for their attractive appearance and abundance of anthocyanins. However, the molecular basis of anthocyanin biosynthesis in red pears remains elusive. Here, a comprehensive transcriptome analysis was conducted to explore the potential regulatory mechanism of anthocyanin biosynthesis in ‘Red Zaosu’ pear (Pyrus pyrifolia × Pyrus communis). Gene co-expression analysis and transcription factor mining identified 263 transcription factors, which accounted for 6.59% of the total number of transcription factors in the pear genome in two gene modules that are highly correlated with anthocyanin biosynthesis. Clustering, gene network modeling with STRING-DB, and local motif enrichment analysis (CentriMo) analysis suggested that PpPIF8 may play a role in anthocyanin biosynthesis. Furthermore, eight PIFs were identified in the pear genome, of which only PpPIF8 was rapidly induced by light. Functional studies showed that PpPIF8 localizes in the nucleus and is preferentially expressed in the tissue of higher levels of anthocyanin. The overexpression of PpPIF8 in pear peel and pear calli promotes anthocyanin biosynthesis and upregulates the expression of anthocyanin biosynthesis genes. Yeast-one hybrid and transgenic analyses indicated that PpPIF8 binds to the PpCHS promoter to induce PpCHS expression. The positive effect of PpPIF8 on anthocyanin biosynthesis is different from previously identified negative regulators of PyPIF5 and MdPIF7 in pear and apple. Taken together, our data not only provide a comprehensive view of transcription events during the coloration of pear peel, but also resolved the regulatory role of PpPIF8 in the anthocyanin biosynthesis pathway.
How to Heal the Gut’s Brain: Regeneration of the Enteric Nervous System
Authors: Rueckert Helen , Ganz Julia
Published: April 2022

The neural-crest-derived enteric nervous system (ENS) is the intrinsic nervous system of the gastrointestinal (GI) tract and controls all gut functions, including motility. Lack of ENS neurons causes various ENS disorders such as Hirschsprung Disease. One treatment option for ENS disorders includes the activation of resident stem cells to regenerate ENS neurons. Regeneration in the ENS has mainly been studied in mammalian species using surgical or chemically induced injury methods. These mammalian studies showed a variety of regenerative responses with generally limited regeneration of ENS neurons but (partial) regrowth and functional recovery of nerve fibers. Several aspects might contribute to the variety in regenerative responses, including observation time after injury, species, and gut region targeted. Zebrafish have recently emerged as a promising model system to study ENS regeneration as larvae possess the ability to generate new neurons after ablation. As the next steps in ENS regeneration research, we need a detailed understanding of how regeneration is regulated on a cellular and molecular level in animal models with both high and low regenerative capacity. Understanding the regulatory programs necessary for robust ENS regeneration will pave the way for using neural regeneration as a therapeutic approach to treating ENS disorders.
Primary Aldosteronism and Resistant Hypertension: A Pathophysiological Insight
Authors: Bioletto Fabio , Bollati Martina , Lopez Chiara , Arata Stefano , Procopio Matteo , Ponzetto Federico , Ghigo Ezio , Maccario Mauro , Parasiliti-Caprino Mirko
Published: April 2022

Primary aldosteronism (PA) is a pathological condition characterized by an excessive aldosterone secretion; once thought to be rare, PA is now recognized as the most common cause of secondary hypertension. Its prevalence increases with the severity of hypertension, reaching up to 29.1% in patients with resistant hypertension (RH). Both PA and RH are “high-risk phenotypes”, associated with increased cardiovascular morbidity and mortality compared to non-PA and non-RH patients. Aldosterone excess, as occurs in PA, can contribute to the development of a RH phenotype through several mechanisms. First, inappropriate aldosterone levels with respect to the hydro-electrolytic status of the individual can cause salt retention and volume expansion by inducing sodium and water reabsorption in the kidney. Moreover, a growing body of evidence has highlighted the detrimental consequences of “non-classical” effects of aldosterone in several target tissues. Aldosterone-induced vascular remodeling, sympathetic overactivity, insulin resistance, and adipose tissue dysfunction can further contribute to the worsening of arterial hypertension and to the development of drug-resistance. In addition, the pro-oxidative, pro-fibrotic, and pro-inflammatory effects of aldosterone may aggravate end-organ damage, thereby perpetuating a vicious cycle that eventually leads to a more severe hypertensive phenotype. Finally, neither the pathophysiological mechanisms mediating aldosterone-driven blood pressure rise, nor those mediating aldosterone-driven end-organ damage, are specifically blocked by standard first-line anti-hypertensive drugs, which might further account for the drug-resistant phenotype that frequently characterizes PA patients.