bluuebunny/crossref_metadata_2025 huggingface dataset:
Urolithin A Inactivation of TLR3/TRIF Signaling to Block the NF-κB/STAT1 Axis Reduces Inflammation and Enhances Antioxidant Defense in Poly(I:C)-Induced RAW264.7 Cells
Authors: Huang Wen-Chung , Liou Chian-Jiun , Shen Szu-Chuan , Hu Sindy , Chao Jane C-J , Hsiao Chien-Yu , Wu Shu-Ju
Published: April 2022

Urolithin A is an active compound of gut-microbiota-derived metabolites of polyphenol ellagic acid that has anti-aging, antioxidative, and anti-inflammatory effects. However, the effects of urolithin A on polyinosinic acid-polycytidylic acid (poly(I:C))-induced inflammation remain unclear. Poly(I:C) is a double-stranded RNA (dsRNA) similar to a virus and is recognized by Toll-like receptor-3 (TLR3), inducing an inflammatory response in immune cells, such as macrophages. Inflammation is a natural defense process of the innate immune system. Therefore, we used poly(I:C)-induced RAW264.7 cells and attenuated the inflammation induced by urolithin A. First, our data suggested that 1–30 μM urolithin A does not reduce RAW264.7 cell viability, whereas 1 μM urolithin A is sufficient for antioxidation and the decreased production of tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and C-C chemokine ligand 5. The inflammation-related proteins cyclooxygenase-2 and inducible nitric oxide synthase were also downregulated by urolithin A. Next, 1 μM urolithin A inhibited the levels of interferon (INF)-α and INF-β. Urolithin A was applied to investigate the blockade of the TLR3 signaling pathway in poly(I:C)-induced RAW264.7 cells. Moreover, the TLR3 signaling pathway, subsequent inflammatory-related pathways, and antioxidation pathways showed changes in nuclear factor-κB (NF-κB) signaling and blocked ERK/mitogen-activated protein kinase (MAPK) signaling. Urolithin A enhanced catalase (CAT) and superoxide dismutase (SOD) activities, but decreased malondialdehyde (MDA) levels in poly(I:C)-induced RAW264.7 cells. Thus, our results suggest that urolithin A inhibits TLR3-activated inflammatory and oxidative-associated pathways in macrophages, and that this inhibition is induced by poly(I:C). Therefore, urolithin A may have antiviral effects and could be used to treat viral-infection-related diseases.
Phylogeny and Biogeographic History of Parnassius Butterflies (Papilionidae: Parnassiinae) Reveal Their Origin and Deep Diversification in West China
Authors: Zhao Youjie , He Bo , Tao Ruisong , Su Chengyong , Ma Junye , Hao Jiasheng , Yang Qun
Published: April 2022

We studied 239 imagoes of 12 Parnassius species collected from the mountains of the Qinghai–Tibet Plateau (QTP) and its neighbouring areas in China. We selected three mitochondrial gene (COI, ND1, and ND5) sequences, along with the homologous gene sequences of other Parnassius species from GenBank, to reconstruct the phylogenetic tree and biogeographic history of this genus. Our results show that Parnassius comprises eight monophyletic subgenera, with subgenus Parnassius at the basal position; the genus crown group originated during the Middle Miocene (ca. 16.99 Ma), and species diversification continued during sustained cooling phases after the Middle Miocene Climate Optimum (MMCO) when the QTP and its neighbouring regions experienced rapid uplift and extensive orogeny. A phylogenetic network analysis based on transcriptomes from GenBank suggests that ancient gene introgression might have contributed to the spread of the Parnassius genus to different altitudes. Ancestral area reconstruction indicates that Parnassius most likely originated in West China (QTP and Xinjiang) and then spread to America in two dispersal events as subgenera Driopa and Parnassius, along with their host plants Papaveraceae and Crassulaceae, respectively. Our study suggests that extensive mountain-building processes led to habitat fragmentation in the QTP, leading to the early diversification of Parnassius, and climate cooling after MMCO was the driving mechanism for the dispersal of Parnassius butterflies from West China to East Asia, Europe, and North America.
Genetic Characteristics According to Subgroup of Acute Myeloid Leukemia with Myelodysplasia-Related Changes
Authors: Kang Dain , Jung Jin , Park Silvia , Cho Byung-Sik , Kim Hee-Je , Kim Yeojae , Lee Jong-Mi , Kim Hoon Seok , Ahn Ari , Kim Myungshin , Kim Yonggoo
Published: April 2022

Acute myeloid leukemia with myelodysplasia-related changes (AML-MRC) includes heterogeneous conditions such as previous history and specific cytogenetic and morphological properties. In this study, we analyze genetic aberrations using an RNA-based next-generation sequencing (NGS) panel assay in 45 patients with AML-MRC and detect 4 gene fusions of KMT2A-SEPT9, KMT2A-ELL, NUP98-NSD1, and RUNX1-USP42 and 81 somatic mutations. Overall, all patients had genetic aberrations comprising of not only cytogenetic changes, but also gene fusions and mutations. We also demonstrated several characteristic genetic mutations according to the AML-MRC subgroup. TP53 was the most commonly mutated gene (n = 11, 24%) and all were found in the AML-MRC subgroup with myelodysplastic syndrome-defining cytogenetic abnormalities (AML-MRC-C) (p = 0.002). These patients showed extremely poor overall survival not only in AML-MRC, but also within the AML-MRC-C subgroup. The ASXL1 (n = 9, 20%) and SRSF2 (n = 7, 16%) mutations were associated with the AML-MRC subgroup with >50% dysplasia in at least two lineages (AML-MRC-M) and were frequently co-mutated (55%, 6/11, p < 0.001). Both mutations could be used as surrogate markers to diagnose AML-MRC, especially when the assessment of multilineage dysplasia was difficult. IDH1/IDH2 (n = 13, 29%) were most commonly mutated in AML-MRC, followed by CEBPA (n = 5, 11%), PTPN11 (n = 5, 11%), FLT3 (n = 4, 9%), IDH1 (n = 4, 9%), and RUNX1 (n = 4, 9%). These mutations were not limited in any AML-MRC subgroup and could have more significance as a risk factor or susceptibility marker for target therapy in not only AML-MRC, but also other AML categories.
The Effects of Anthropogenic Electromagnetic Fields (EMF) on the Early Development of Two Commercially Important Crustaceans, European Lobster, Homarus gammarus (L.) and Edible Crab, Cancer pagurus (L.)
Authors: Harsanyi Petra , Scott Kevin , Easton Blair A. A. , de la Cruz Ortiz Guadalupe , Chapman Erica C. N. , Piper Althea J. R. , Rochas Corentine M. V. , Lyndon Alastair R.
Published: April 2022

Proposed offshore windfarm sites could overlap with the brooding and spawning habitats of commercially important crustacea, including European lobster, Homarus gammarus and Edible crab, Cancer pagurus. Concerns have been raised on the biological effects of Electromagnetic Fields (EMFs) emitted from subsea power cables on the early life history of these species. In this study, ovigerous female H. gammarus and C. pagurus were exposed to static (Direct Current, DC) EMFs (2.8 mT) throughout embryonic development. Embryonic and larval parameters, deformities, and vertical swimming speed of freshly hatched stage I lobster and zoea I crab larvae were assessed. EMF did not alter embryonic development time, larval release time, or vertical swimming speed for either species. Chronic exposure to 2.8 mT EMF throughout embryonic development resulted in significant differences in stage-specific egg volume and resulted in stage I lobster and zoea I crab larvae exhibiting decreased carapace height, total length, and maximum eye diameter. An increased occurrence of larval deformities was observed in addition to reduced swimming test success rate amongst lobster larvae. These traits may ultimately affect larval mortality, recruitment and dispersal. This study increases our understanding on the effects of anthropogenic, static EMFs on crustacean developmental biology and suggests that EMF emissions from subsea power cables could have a measurable impact on the early life history and consequently the population dynamics of H. gammarus and C. pagurus.
Recent Progress on Wave Energy Marine Buoys
Authors: Xu Ruijiang , Wang Hao , Xi Ziyue , Wang Weichen , Xu Minyi
Published: April 2022

This study aims to introduce and discuss the recent research, development and application of wave energy marine buoys. The topic becomes increasingly appealing after the observation that wave energy technologies have been evolving in the recent decades, yet have not reached convergence. The power supply is usually the bottleneck for marine distributed systems such as buoys. Wave energy technologies are especially useful in this sense, as they can capture and convert the promising “native” renewable energy in the ocean (i.e., wave energy) into electricity. The paper enumerates the recent developments in wave energy capture (e.g., oscillating bodies) and power take-off (e.g., nanogenerators). The study also introduces the typical marine buoys and discusses the applicability of wave energy technologies on them. It is concluded that the wave energy technologies could be implemented as a critical addition to the comprehensive power solution of marine distributed systems. Wave energy buoys are likely to differentiate into “wave energy converter buoys” and “wave-energy-powered buoys”, which is indicated by the ratio of the generated power to the load power.
Validation and Ecological Niche Investigation of a New Fungal Intraspecific Competitor as a Biocontrol Agent for the Sustainable Containment of Aflatoxins on Maize Fields
Authors: Spadola Giorgio , Giannelli Gianluigi , Magagnoli Serena , Lanzoni Alberto , Albertini Marco , Nicoli Riccardo , Ferrari Roberto , Burgio Giovanni , Restivo Francesco M. , Degola Francesca
Published: April 2022

Crop yield and plant products quality are directly or indirectly affected by climate alterations. Adverse climatic conditions often promote the occurrence of different abiotic stresses, which can reduce or enhance the susceptibility to pests or pathogens. Aflatoxin producing fungi, in particular, whose diffusion and deleterious consequences on cereals commodities have been demonstrated to highly depend on the temperature and humidity conditions that threaten increasingly larger areas. Biological methods using intraspecific competitors to prevent fungal development and/or toxin production at the pre-harvest level are particularly promising, even if their efficacy could be affected by the ecological interaction within the resident microbial population. A previously characterized Aspergillus flavus atoxigenic strain was applied in two maize fields to validate its effectiveness as a biocontrol agent against aflatoxin contamination. At one month post-application, at the harvest stage, its persistence within the A. flavus population colonizing the maize kernels in the treated area was assessed, and its efficacy was compared in vitro with a representation of the isolated atoxigenic population. Results proved that our fungal competitor contained the aflatoxin level on maize grains as successfully as a traditional chemical strategy, even if representing less than 30% of the atoxigenic strains re-isolated, and achieved the best performance (in terms of bio-competitive potential) concerning endogenous atoxigenic isolates.
Mechanistic Basis of Super-Infection: Influenza-Associated Invasive Pulmonary Aspergillosis
Authors: Robinson Keven Mara
Published: April 2022

Influenza infection is a risk factor for invasive pulmonary aspergillosis in both immunocompetent and immunocompromised hosts. The purpose of this review is to highlight the epidemiology of influenza-associated invasive pulmonary aspergillosis and the mechanistic studies that have been performed to delineate how influenza increases susceptibility to this invasive fungal infection.
Transcriptional Profiles Elucidate Differential Host Responses to Infection with Cryptococcus neoformans and Cryptococcus gattii
Authors: Holcomb Zachary E. , Steinbrink Julie M. , Zaas Aimee K. , Betancourt Marisol , Tenor Jennifer L. , Toffaletti Dena L. , Alspaugh J. Andrew , Perfect John R. , McClain Micah T.
Published: April 2022

Many aspects of the host response to invasive cryptococcal infections remain poorly understood. In order to explore the pathobiology of infection with common clinical strains, we infected BALB/cJ mice with Cryptococcus neoformans, Cryptococcus gattii, or sham control, and assayed host transcriptomic responses in peripheral blood. Infection with C. neoformans resulted in markedly greater fungal burden in the CNS than C. gattii, as well as slightly higher fungal burden in the lungs. A total of 389 genes were significantly differentially expressed in response to C. neoformans infection, which mainly clustered into pathways driving immune function, including complement activation and TH2-skewed immune responses. C. neoformans infection demonstrated dramatic up-regulation of complement-driven genes and greater up-regulation of alternatively activated macrophage activity than seen with C gattii. A 27-gene classifier was built, capable of distinguishing cryptococcal infection from animals with bacterial infection due to Staphylococcus aureus with 94% sensitivity and 89% specificity. Top genes from the murine classifiers were also differentially expressed in human PBMCs following infection, suggesting cross-species relevance of these findings. The host response, as manifested in transcriptional profiles, informs our understanding of the pathophysiology of cryptococcal infection and demonstrates promise for contributing to development of novel diagnostic approaches.
A Spatial Analysis of the Spread of Hyperendemic Sporotrichosis in the State of Rio de Janeiro, Brazil
Authors: Falcão Eduardo Mastrangelo Marinho , Romão Anselmo Rocha , Magalhães Mônica de Avelar Figueiredo Mafra , de Lima Filho José Berilo , do Valle Antonio Carlos Francesconi , Bastos Francisco Inácio , Gutierrez-Galhardo Maria Clara , Freitas Dayvison Francis Saraiva
Published: April 2022

Sporotrichosis is a subacute/chronic subcutaneous mycosis. Since the late 1990s, there has been a hyperendemic zoonotic transmission in the state of Rio de Janeiro, involving Sporothrix brasiliensis, the most virulent causative species, and a “belt” was described along the limits between the capital and its outskirts (“Baixada Fluminense”). This study analyzes the distribution of sporotrichosis using secondary data from the Notifiable Diseases Information System (Sinan) of the Rio de Janeiro State Health Department (SES/RJ) from 2011 to 2015 and from the INI Electronic Patient Record System (Sipec) from 2008 to 2015. Cases diagnosed since the onset of the hyperendemic exceed all previously reported case series of the disease and there is a progressive expansion in the state of Rio de Janeiro. The study suggests the spread of the mycosis to all regions of the state and the expansion of the previously described “belt”, despite public health measures and changes in its profile over the years, with great social impact.
Serum Cytokines Usefulness for Understanding the Pathology in Allergic Bronchopulmonary Aspergillosis and Chronic Pulmonary Aspergillosis
Authors: Ito Yuya , Takazono Takahiro , Obase Yasushi , Fukahori Susumu , Ashizawa Nobuyuki , Hirayama Tatsuro , Tashiro Masato , Yamamoto Kazuko , Imamura Yoshifumi , Hosogaya Naoki , Fukushima Chizu , Morinaga Yoshitomo , Yanagihara Katsunori , Izumikawa Koichi , Mukae Hiroshi
Published: April 2022

Allergic bronchopulmonary aspergillosis (ABPA) and chronic pulmonary aspergillosis (CPA) are important fungal infections caused by Aspergillus species. An overlap of ABPA and CPA has been reported; therefore, it is critical to determine whether the main pathology is ABPA or CPA and whether antifungals are required. In this study, we investigated whether the serum cytokine profile is useful for understanding the pathology and for differentiating between these diseases. We compared the various serum cytokine levels among healthy subjects and patients diagnosed with asthma, ABPA, or CPA at Nagasaki University Hospital between January 2003 and December 2018. In total, 14 healthy subjects, 19 patients with asthma, 11 with ABPA, and 10 with CPA were enrolled. Interleukin (IL) -5 levels were significantly higher in patients with ABPA than in those with CPA, and IL-33 and tumor necrosis factor (TNF) levels were significantly higher in patients with CPA than in those with asthma (p < 0.05, Dunn’s multiple comparison test). The sensitivity and specificity of the IL-10/IL-5 ratio (cutoff index 2.47) for diagnosing CPA were 70% and 100%, respectively. The serum cytokine profile is useful in understanding the pathology of ABPA and CPA, and the IL-10/IL-5 ratio may be a novel supplemental biomarker for indicating the pathology of CPA.
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH
Authors: Alves Mariana Fuinhas , Murray Patrick G.
Published: April 2022

Fungi’s ability to convert organic materials into bioactive products offers environmentally friendly solutions for diverse industries. In the nanotechnology field, fungi metabolites have been explored for green nanoparticle synthesis. Silver nanoparticle (AgNP) research has grown rapidly over recent years mainly due to the enhanced optical, antimicrobial and anticancer properties of AgNPs, which make them extremely useful in the biomedicine and biotechnology field. However, the biological synthesis mechanism is still not fully established. Therefore, this study aimed to evaluate the combined effect of time, temperature and pH variation in AgNP synthesis using three different fungi phyla (Ascomycota, Basidiomycota and Zygomycota) represented by six different fungi species: Cladophialophora bantiana (C. bantiana), Penicillium antarcticum (P. antarcticum), Trametes versicolor (T. versicolor), Trichoderma martiale (T. martiale), Umbelopsis isabellina (U. isabellina) and Bjerkandera adusta (B. adusta). Ultraviolet–visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM) results demonstrated the synthesis of AgNPs of different sizes (3 to 17 nm) and dispersity percentages (25 to 95%, within the same size range) using fungi extracts by changing physicochemical reaction parameters. It was observed that higher temperatures (90 °C) associated with basic pH (9 and 12) favoured the synthesis of monodisperse small AgNPs. Previous studies demonstrated enhanced antibacterial and anticancer properties correlated with smaller nanoparticle sizes. Therefore, the biologically synthesised AgNPs shown in this study have potential as sustainable substitutes for chemically made antibacterial and anticancer products. It was also shown that not all fungi species (B. adusta) secrete metabolites capable of reducing silver nitrate (AgNO3) precursors into AgNPs, demonstrating the importance of fungal screening studies.
Cell Lines of Circulating Tumor Cells: What Is Known and What Needs to Be Resolved
Authors: Shimada Yutaka , Sudo Tetsuo , Akamatsu Shusuke , Sunada Takuro , Myomoto Akira , Okano Kiyoshi , Shimizu Kazuharu
Published: April 2022

The importance of circulating tumor cells (CTC) is well recognized. However, the biological characteristics of CTC in the bloodstream have not yet been examined in detail, due to the limited number of CTC cell lines currently available. Thirty-nine CTC cell lines were reported by 2021. For successful cell culturing, these CTC cell lines were reviewed. Previous studies on short-term cultures of CTC also analyzed approaches for establishing the long-term culture of CTC. Negative selection, hypoxic conditions, three-dimensional conditions, and careful management are preferable for the long-term culture of CTC. However, the establishment of CTC cell lines is dependent on the specific characteristics of each cell type. Therefore, a method to establish CTC cell lines has not yet been developed. Further efforts are needed to resolve this issue.
A Comparative Analysis of the Economic Sustainability of Cultural Work in the UK since the COVID-19 Pandemic and Examination of Universal Basic Income as a Solution for Cultural Workers
Authors: Doustaly Cécile , Roy Vishalakshi
Published: April 2022

The COVID-19 pandemic and related lockdowns across the world have greatly affected an already vulnerable cultural economy and the structural precarity of many cultural workers. After documenting the impacts of the pandemic in the cultural sector and the effectiveness of governmental responses in the UK and in Europe, the article focuses on the visual arts and explores calls for reforms of the cultural economy. While the UK government’s recovery plan went against the country’s cultural policy tradition due to the plan’s interventionist and financially generous nature, it disproportionally benefitted organisations rather than individuals working in the sector, especially in England. The study, conducted on visual arts workers in the UK, shows that many were unable to access these financial recovery schemes and fell through the cracks of the complex criteria set for these funds. This article informs the current debate on measures that are potentially more economically sustainable and wellbeing protective than those currently in place for cultural workers, such as Universal Basic Income. Its applicability is explored with reference to the historic French and recent Irish examples.
Downscaling Switzerland Land Use/Land Cover Data Using Nearest Neighbors and an Expert System
Authors: Giuliani Gregory , Rodila Denisa , Külling Nathan , Maggini Ramona , Lehmann Anthony
Published: April 2022

High spatial and thematic resolution of Land Use/Cover (LU/LC) maps are central for accurate watershed analyses, improved species, and habitat distribution modeling as well as ecosystem services assessment, robust assessments of LU/LC changes, and calculation of indices. Downscaled LU/LC maps for Switzerland were obtained for three time periods by blending two inputs: the Swiss topographic base map at a 1:25,000 scale and the national LU/LC statistics obtained from aerial photointerpretation on a 100 m regular lattice of points. The spatial resolution of the resulting LU/LC map was improved by a factor of 16 to reach a resolution of 25 m, while the thematic resolution was increased from 29 (in the base map) to 62 land use categories. The method combines a simple inverse distance spatial weighting of 36 nearest neighbors’ information and an expert system of correspondence between input base map categories and possible output LU/LC types. The developed algorithm, written in Python, reads and writes gridded layers of more than 64 million pixels. Given the size of the analyzed area, a High-Performance Computing (HPC) cluster was used to parallelize the data and the analysis and to obtain results more efficiently. The method presented in this study is a generalizable approach that can be used to downscale different types of geographic information.
Suitability Prediction and Enhancement of Future Water Supply Systems in Barwon Region in Victoria, Australia
Authors: Zhang Shihao , Tan Junhe , Liu Junhang , Wang Jiaqi , Tara Ata
Published: April 2022

Intensive agricultural production accompanied by the climate change impacts in post-Colonial rural landscapes have continuously increased the demand for water resources and coastal areas, showing an unprecedented water supply crisis. By taking extreme weather conditions and rainfall events for future trends, a resilient water storage facility for the landscape requires the collaborative approach of natural systems and simulation modelling techniques to develop sustainable future scenarios. In this study, an ecological suitability model is used to identify potential sites for the construction of multi-purpose dams. As part of the model structure, multi factors are classified using the patterns of changing landscapes, and then weighted overlay analysis is conducted on a Geographic Information System (GIS) platform. Compared to previous studies, this paper derives its principal impact parameters and projections based on historical land cover information. The suitability maps that are generated visually guide the geographical location of the multi-purpose dams and indicate the areas from highly suitable to least suitable, clarifying the possibility of building blue infrastructure alongside the waterways in west-central Barwon. The workflow proposes a resilient water system based on existing land characteristics and measures that future water storage capacity will be a valid increase of approximately 1.5 times. This strategy alleviates water scarcity during the dry season to benefit traditional agricultural activities. Digital calculations are utilized to demonstrate the feasibility of the experimental results, providing a methodology for regulating the distribution and supply of river flows throughout the year while retaining runoff in a hierarchical pattern at precipitation periods.
The Spell-Out of Non-Heads in Spanish Compounds: A Nanosyntactic Approach
Authors: Marqueta Gracia Bárbara
Published: April 2022

This paper considers the morphophonological variants found in the non-head position of Spanish productive compound patterns. In the literature it has been noted that compounds resort to both I- and O-ending stems and words (even inflected ones) to spell-out compound non-heads. The study takes a nanosyntactic approach to compound syntax and examines the functional structure of the main classes of Spanish compounds. The goal of the analysis is to show that the functional structures of compounds determine to some extent which spell-outs are chosen in lexical innovations within the schemas, and also to address the issue of why certain allomorphs never appear in a given context while others seem to compete for lexical insertion. Research was conducted using a self-compiled sample of 1250 compounds extracted from contemporary Spanish written corpora. The results provide empirical support for syntacticist approaches to compounding, in that the proposed functional structures capture the predominantly compositional semantics of compounding, and also because traditional “linking vowels” are reconsidered to be the stable and systematic spell-outs for relational categories inside compounds.
Protecting Asylum Seekers and Migrants in the Context of the Rule of Law Crisis in EU Member States: The Recent Approach of the Court of Justice of the EU through the Lens of the Global Compacts on Refugees and Migration
Authors: Favi Alessandra
Published: April 2022

In recent times, the CJEU has started to develop its judicial response to the “rule of law crisis” in some Member States. On the one hand, this new trend has emerged also as a reaction to some national reforms concerning asylum and migration law. On the other hand, the CJEU in protecting the EU founding values has deployed its “traditional” competences attributed to it by the EU Treaties, namely the mechanisms of the preliminary ruling procedure and the infringement procedure. Against this background, this contribution aims at investigating this new CJEU’s jurisprudence through the lens of the Global Compacts on Refugees and Migration. This will lead us to reflect on how the CJEU’s caselaw could be seen as an effective tool to enhance the rule of law and protect third-country nationals, at least within the EU, and indirectly contributes to increasing compliance with some of the commitments laid down in the Global Compacts, regardless of the position taken by some recalcitrant EU Member States with respect to these documents.
Directing the Legal Radar at Forced Labour—Under Special Consideration of Male Victims in Norway
Authors: Lingaas Carola
Published: April 2022

Human trafficking in the form of labour exploitation appears to have gone under the legal radar domestically, regionally, and internationally, with ensuing grave consequences for the victims concerned. This paper critically discusses the current legal developments and interpretations of global and regional legal sources on forced labour and the challenges they face. A legal analysis is supplemented by information obtained through interviews with 14 presumed male victims of forced labour, who recently escaped a coercive work situation and were living in a safe house in Oslo (Norway). The paper will demonstrate the shortcomings of the law and its application, using the case of Norway and the affected men as an example. It examines the case law of the European Court of Human Rights using a vulnerability approach and argues that the inaction in preventing and prosecuting crimes committed towards people who are exploited for forced labour is a violation of their human rights and may be interpreted as granting impunity to their perpetrators. The situation for male victims of forced labour is particularly severe.
Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
Authors: Pawar Harshal D. , Mahajan Umesh B. , Nakhate Kartik T. , Agrawal Yogeeta O. , Patil Chandragouda R. , Meeran M. F. Nagoor , Sharma Charu , Ojha Shreesh , Goyal Sameer N.
Published: April 2022

Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-fat diet (HFD) throughout the experiment (30 days). Following 7 days of HFD feeding, streptozotocin was administered (150 mg/kg, intraperitoneal) to induce diabetes. From day 11 to 30, diabetic mice received either curcumin (100 or 200 mg/kg/day, oral), CB2R antagonist AM630 (1 mg/kg/day, intraperitoneal) or both, with concurrent isoproterenol (150 mg/kg, subcutaneous) administration on day 28 and 29. Diabetic mice with myocardial infarction showed an altered hemodynamic pattern and lipid profile, reduced injury markers, antioxidants with increased lipid peroxidation in the myocardium, and elevated glucose and liver enzymes in the blood. Moreover, an increased pro-inflammatory markers, histological severity, myonecrosis, and edema were observed. Curcumin compensated for hemodynamic fluctuations, restored biochemical markers, preserved antioxidant capacity, decreased cytokines levels, and restored cardiac functionality. However, the AM630 pre-treatment attenuated the effects of curcumin. The data suggest the involvement of CB2R in the actions of curcumin such as in the prevention of myocardial stress and in the improvement of the normal status of the myocardial membrane associated with diabetes.
Spleen: Reparative Regeneration and Influence on Liver
Authors: Elchaninov Andrey , Vishnyakova Polina , Sukhikh Gennady , Fatkhudinov Timur
Published: April 2022

This review considers experimental findings on splenic repair, obtained in two types of small animal (mouse, rat, and rabbit) models: splenic resections and autologous transplantations of splenic tissue. Resection experiments indicate that the spleen is able to regenerate, though not necessarily to the initial volume. The recovery lasts one month and preserves the architecture, albeit with an increase in the relative volume of lymphoid follicles. The renovated tissues, however, exhibit skewed functional profiles; notably, the decreased production of antibodies and the low cytotoxic activity of T cells, consistent with the decline of T-dependent zones and prolonged reduction in T cell numbers. Species–specific differences are evident as well, with the post-repair organ mass deficiency most pronounced in rabbit models. Autotransplantations of splenic material are of particular clinical interest, as the procedure can possibly mitigate the development of post-splenectomy syndrome. Under these conditions, regeneration lasts 1–2 months, depending on the species. The transplants effectively destroy senescent erythrocytes, assist in microbial clearance, and produce antibodies, thus averting sepsis and bacterial pneumonia. Meanwhile, cellular sources of splenic recovery in such models remain obscure, as well as the time required for T and B cell number reconstitution.
Construction of PIK3C3 Transgenic Pig and Its Pathogenesis of Liver Damage
Authors: Wang Jing , Khan Sami Ullah , Cao Pan , Chen Xi , Wang Fengchong , Zou Di , Li Honghui , Zhao Heng , Xu Kaixiang , Jiao Deling , Yang Chang , Zhu Feiyan , Zhang Yaxuan , Su Yanhua , Cheng Wenmin , Jia Baoyu , Qing Yubo , Jamal Muhammad Ameen , Zhao Hong-Ye , Wei Hong-Jiang
Published: April 2022

As a member of the PIKs family, PIK3C3 participates in autophagy and plays a central role in liver function. Several studies demonstrated that the complete suppression of PIK3C3 in mammals can cause hepatomegaly and hepatosteatosis. However, the function of PIK3C3 overexpression on the liver and other organs is still unknown. In this study, we successfully generated PIK3C3 transgenic pigs through somatic cell nuclear transfer (SCNT) by designing a specific vector for the overexpression of PIK3C3. Plasmid identification was performed through enzyme digestion and transfected into the fetal fibroblasts derived from Diannan miniature pigs. After 2 weeks of culturing, six positive colonies obtained from a total of 14 cell colonies were identified through PCR. One positive cell line was selected as the donor cell line for SCNT for the construction of PIK3C3transgenic pigs. Thirty single blastocysts were collected and identified as PIK3C3 transgenic-positive blastocysts. Two surrogates became pregnant after transferring the reconstructed embryos into four surrogates. Fetal fibroblasts of PIK3C3-positive fetuses identified through PCR were used as donor cells for SCNT to generate PIK3C3 transgenic pigs. To further explore the function of PIK3C3 overexpression, genotyping and phenotyping of the fetuses and piglets obtained were performed by PCR, immunohistochemical, HE, and apoptosis staining. The results showed that inflammatory infiltration and vacuolar formation in hepatocytes and apoptotic cells, and the mRNA expression of NF-κB, TGF-β1, TLR4, TNF-α, and IL-6 significantly increased in the livers of PIK3C3 transgenic pigs when compared with wild-type (WT) pigs. Immunofluorescence staining showed that LC3B and LAMP-1-positive cells increased in the livers of PIK3C3 transgenic pigs. In the EBSS-induced autophagy of the porcine fibroblast cells (PFCs), the accumulated LC3II protein was cleared faster in PIK3C3 transgenic (PFCs) thanWT (PFCs). In conclusion, PIK3C3 overexpression promoted autophagy in the liver and associated molecular mechanisms related to the activation of ULK1, AMBR1, DRAM1, and MTOR, causing liver damage in pigs. Therefore, the construction of PIK3C3 transgenic pigs may provide a new experimental animal resource for liver diseases.
A Concise Synthesis towards Antimalarial Quinazolinedione TCMDC-125133 and Its Anti-Proliferative Activity against MCF-7
Authors: Charoensutthivarakul Sitthivut , Lohawittayanan Duangporn , Kanjanasirirat Phongthon , Jearawuttanakul Kedchin , Seemakhan Sawinee , Borwornpinyo Suparerk , Phanchana Matthew
Published: April 2022

Quinazolinedione is one of the most notable pharmacophores in drug discovery due to its broad spectrum of biological activities including antimalarial, anticancer, anti-inflammatory, and others. TCMDC-125133, whose structure features a quinazolinedione core, exhibits promising antimalarial activity and low toxicity as described in the GlaxoSmithKline (GSK) report. Herein, a concise four-step synthesis towards quinazolinedione TCMDC-125133 is described using low cost goods and greener alternatives where possible. All synthesized compounds were characterized using polarimetry, IR, NMR, and mass spectrometry. The in-house synthesized TCMDC-125133 was evaluated for its antimalarial activity against P. falciparum 3D7 and antiproliferative activity against MCF-7 cell line.
Glassy Carbon Electrode Modified with N-Doped Reduced Graphene Oxide Sheets as an Effective Electrochemical Sensor for Amaranth Detection
Authors: Moradpour Hediyeh , Beitollahi Hadi , Nejad Fariba Garkani , Di Bartolomeo Antonio
Published: April 2022

Amaranth is one of the synthetic azo colorants used to improve the appearance and to increase the appeal of some foods and soft drinks. The excessive consumption of amaranth can be associated with health side effects, emphasizing the need to monitor this food dye. Accordingly, the present study aimed to introduce an electrochemical sensor of glassy carbon electrode (GCE) modified with N-doped reduced graphene oxide (N-rGO), N-rGO/GCE, to detect the amaranth sensitively and rapidly. Several electrochemical techniques such as differential pulse voltammetry (DPV), linear sweep voltammetry (LSV), chronoamperometry (CHA), and cyclic voltammetry (CV) are exploited for the evaluation of the efficiency of the developed electrode for the detection of amaranth. We found that N-rGO/GCE enhanced amaranth oxidation, thus significantly elevating the current signal. Amaranth showed that calibration curves ranged from 0.1 to 600.0 µM, and the limit of detection (LOD) (S/N = 3) was 0.03 μM. Finally, the developed sensor was effectively applied for real samples (tap water, apple juice, and orange juice) with acceptable recovery values from 96.0 to 104.3%.
Magnetic Anisotropy and Microstructure in Electrodeposited Quaternary Sn-Fe-Ni-Co Alloys with Amorphous Character
Authors: Kuzmann Ernő , Felner Israel , Sziráki Laura , Stichleutner Sándor , Homonnay Zoltán , El-Sharif Mahmoud R. , Chisholm Colin U.
Published: April 2022

Sn-Fe-Ni-Co quaternary alloys, in the composition range of 37–44 at% Sn, 35–39 at% Fe, 6–8 at% Ni and 13–17 at% Co, were prepared by direct current (DC) and pulse plating (PP) electrodeposition. The alloy deposits were characterized by XRD, 57Fe and 119Sn conversion electron Mössbauer spectroscopy, SEM-EDX and magnetization measurements. XRD revealed the amorphous character of the quaternary alloy deposits. The dominant ferromagnetic character of the deposits was shown by magnetization and Mössbauer spectroscopy measurements. Room temperature Mössbauer spectra showed minor paramagnetic phases, where their occurrences (~3–20%) are correlated to the electrodeposition parameters (Jdep from −16 to −23 mA/cm2 for DC, Jpulse from −40 to −75 mA/cm2 for PP), the composition and the saturation magnetization (~52–73 emu/g). A considerable difference was found in the magnetization curves applying parallel or perpendicular orientation of the applied fields, indicating magnetic anisotropy both in DC and pulse plated alloy coatings.
Effect of Various Fly Ash and Ground Granulated Blast Furnace Slag Content on Concrete Properties: Experiments and Modelling
Authors: Qu Zhiwei , Liu Zihao , Si Ruizhe , Zhang Yingda
Published: April 2022

Concrete is known as the most globally used construction material, but it releases a huge amount of greenhouse gases due to cement production. Recently, Supplementary Cementitious Materials (SCMs) such as fly ash and Ground Granulated Blast Furnace Slag (GGBFS) have been widely used in concrete to reduce the cement content. However, SCMs can alter the mechanical properties and time-dependent behaviors of concrete and the early age mechanical properties of concrete significantly affect the concrete cracking in the engineering field. Therefore, evaluation of the development of the mechanical properties of SCMs-based concrete is vital. In this paper, the time development of mechanical properties of concrete mixes with various fly ash and GGBFS was experimentally investigated. Four different cement replacement levels including 0%, 20%, 30%, and 40% by fly ash and GGBFS as well as ternary binders were considered. Compressive strength, splitting tensile strength, flexural strength, and elastic modulus of concrete were measured until 28 days. Three additional concrete mixes with ternary binders were also cast to investigate the early-age autogenous shrinkage development until 28 days. In addition, prediction models in existing standards were used and compared to experimental results. The comparison results showed that the prediction models overestimated the compressive strength but underestimated the splitting tensile strength development and autogenous shrinkage. As a result, a model capturing the effect of fly ash and GGBFS on the development of compressive and splitting tensile strength is proposed to improve the prediction accuracy for current standards and empirical models.
Influencing the Size and Shape of High-Energy Ball Milled Particle Reinforced Aluminum Alloy Powder
Authors: Trautmann Maik , Ahmad Husam , Wagner Guntram
Published: April 2022

High-energy ball milling represents an efficient process for producing composite powders consisting of ceramic particles dispersed in a metallic matrix. However, collision events, plastic deformations, and cold welding during the milling lead to a flake or block-like shape of the resulting composite powders. Further consolidation of such irregularly shaped powders by powder bed-based additive manufacturing technologies can be challenging because of their low flowability and low bulk density. In this work, different approaches, including milling process parameters (speed, process control agent atmosphere) and post-treatments (mechanical and thermal), are investigated on their suitability to influence the particle shape, especially concerning the roundness of the composite powders consisting of the aluminum alloy AlSi10Mg with 5 vol% SiC and Al2O3 reinforcement. It is found that milling with menthol as a process control agent leads to the finest composite powder compared to other milling parameters, with the lowest particle roundness of 0.39 (initial powders 0.84). No success in rounding the milled composite powder could be achieved through mechanical post-treatment in a planetary ball mill. On the other side, the thermal spraying of, e.g., SiC reinforced AlSi10Mg powder resulted in a 77–82% relative roundness. A remarkable change in the microstructure and the shape of the composite powders could also be observed after heat treatment in tube furnaces at a temperature above the melting point of AlSi10Mg. The best result in terms of improved roundness (relative to around 85%) was obtained for Al2O3 reinforced at 600 °C. A further increase of the temperature to 700 °C resulted in a moderate coarsening of powders with Al2O3 and extensive sintering of powders with SiC, presumably due to a different distribution inside the matrix.
Ash from Poultry Manure Incineration as a Substitute for Phosphorus Fertiliser
Authors: Cempa Magdalena , Olszewski Paweł , Wierzchowski Krzysztof , Kucharski Piotr , Białecka Barbara
Published: April 2022

The goal of the tests was to separate a phosphate concentrate from ash and to assess its fertiliser use efficiency in anthropogenic land. Ash obtained from poultry manure incineration is an interesting fertiliser, as it contains both of the necessary nutrients, i.e., phosphorus and potassium. The ash selected for the tests contained 15.73 wt% P2O5, and 6.75 wt% K2O. CaO also constituted the main component (44.79 wt%). Phosphorus in crystalline form was present as hydroxyapatite and carbonate apatite. The first stage, applied in order to separate a phosphate concentrate from ash, involved a number of physicochemical methods: (i) a method based on grain wettability differences; (ii) a method based on grain density differences; and (iii) methods based on size distribution differences. Wet sieving made it possible to separate a fraction with a P2O5 content of 24.56 wt%. The second stage, applied to assess fertiliser use efficiency, involved cassette tests as well as pot and field cultivation using as fertiliser, the obtained product as well as raw ash and commercial ones. Therefore, the conducted research allowed for the development of a methodology for the management of ash from the incineration of a poultry manure and their use as a substitute for phosphorus fertiliser. The tested material was applied in various doses. Using the obtained phosphate concentrate at a dose of 95 g/m3 resulted in a comparable yield as in the case of the commercial fertiliser at a dose recommended by the producer (75 g/m3). Unprocessed ash had to be used in larger amounts, i.e., 165 g/m3, to have a comparable yield as a commercial fertiliser.
Charge Accumulation in the Homo-Crosslinked-Polyethylene Bilayer
Authors: Zhao Wei , Li Huaqiang , Li Wenpeng , Chen Xin , Zhong Lisheng , Gao Jinghui
Published: April 2022

The homo-crosslinked-polyethylene (H-XLPE) bilayer simplifies the returned insulation structure of the factory joint in submarine cables, and its dielectric property is key to the reliability of the power transmission system. In this paper, we investigated the charge accumulation phenomenon in a secondary thermocompression H-XLPE bilayer using the pulse electro-acoustic method. The charge accumulation reduces its overall breakdown strength when compared with XLPE. According to X-ray diffraction measurement and thermal analysis results, the specimen forms a homo-junction region between the bilayers, which has overlapping spherulites with a thick lamella, high crystallinity, and high surface free energy. The charge accumulation can be ascribed to fused lamellas and the crystal imperfection of the homo-junction region, which restricts the charge transport process and exhibits a higher number of deep traps. This study emphasizes the importance of the homo-junction region in the H-XLPE bilayer, which should be considered in the design and operation of factory joint insulation.
Thermal Properties of Illite-Zeolite Mixtures up to 1100 °C
Authors: Csáki Štefan , Sunitrová Ivana , Lukáč František , Łagód Grzegorz , Trník Anton
Published: April 2022

Illitic clays are the commonly used material in building ceramics. Zeolites are microporous, hydrated crystalline aluminosilicates, they are widely used due to their structure and absorption properties. In this study, illitic clay (Füzérradvány, Hungary) was mixed with natural zeolite (Nižný Hrabovec, Slovakia) with up to 50 wt.% of zeolite content. The samples were submitted to thermal analyses, such as differential thermal analysis, differential scanning calorimetry, thermogravimetry, and dilatometry. In addition, the evolution of thermal diffusivity, thermal conductivity, and specific heat capacity in the heating stage of firing were measured and discussed. The amount of the physically bound water in the samples increased along with the amount of zeolite. The temperature of the illite dehydroxylation (peak temperature) was slightly shifted to lower temperatures, from 609 °C to 575 °C (for sample IZ50). On the other hand, the mass loss and the shrinkage of the samples significantly increased with the zeolite content in the samples. Sample IZ50 reached 10.8% shrinkage, while the sample prepared only from the illitic clay contracted by 5.8%. Nevertheless, the temperature of the beginning of the sintering (taken from the dilatometric curves) decreased from 1021 °C (for illitic clay) to 1005 °C (for IZ50). The thermal diffusivity and thermal conductivity values decreased as the amount of zeolite increased in the samples, thus showing promising thermal insulating properties.
The Supported Boro-Additive Effect for the Selective Recovery of Dy Elements from Rare-Earth-Elements-Based Magnets
Authors: Park Sangmin , Kim Dae-Kyeom , Hussain Javid , Song Myungsuk , Kim Taek-Soo
Published: April 2022

Liquid metal extraction (LME) for recycling rare-earth elements from magnets is studied, in the present study, to examine its suitability as an environmentally friendly alternative for a circular economy. While Nd (neodymium) extraction efficiency can easily reach almost 100%, based on the high reactivity of Mg (magnesium), Dy (dysprosium) extraction has been limited because of the Dy–Fe intermetallic phase as the main extractive bottleneck. In the present paper, the boro-additive effect is designed thermodynamically and examined in the ternary and quinary systems to improve the selectivity of recovery. Based on the strong chemical affinity between B (boron) and Fe, the effect of excess boron, which is produced by the depletion of B in FeB by Mg, successfully resulted in the formation of Fe2B instead of Dy–Fe bonding. However, the growth of the Fe2B layer, which is the reason for the isolated Mg, leads to the production of other byproducts, rare-earth borides (RB4, R = Nd and Dy), as the side effect. By adjusting the ratio of FeB, the extraction efficiency of Dy over 12 h with FeB addition is improved to 80%, which is almost the same extraction efficiency of the conventional LME process over 24 h.