bluuebunny/crossref_metadata_2025 huggingface dataset:
Entropic Statistical Description of Big Data Quality in Hotel Customer Relationship Management
Authors: González-Serrano Lydia , Talón-Ballestero Pilar , Muñoz-Romero Sergio , Soguero-Ruiz Cristina , Rojo-Álvarez José Luis
Published: April 2019

Customer Relationship Management (CRM) is a fundamental tool in the hospitality industry nowadays, which can be seen as a big-data scenario due to the large amount of recordings which are annually handled by managers. Data quality is crucial for the success of these systems, and one of the main issues to be solved by businesses in general and by hospitality businesses in particular in this setting is the identification of duplicated customers, which has not received much attention in recent literature, probably and partly because it is not an easy-to-state problem in statistical terms. In the present work, we address the problem statement of duplicated customer identification as a large-scale data analysis, and we propose and benchmark a general-purpose solution for it. Our system consists of four basic elements: (a) A generic feature representation for the customer fields in a simple table-shape database; (b) An efficient distance for comparison among feature values, in terms of the Wagner-Fischer algorithm to calculate the Levenshtein distance; (c) A big-data implementation using basic map-reduce techniques to readily support the comparison of strategies; (d) An X-from-M criterion to identify those possible neighbors to a duplicated-customer candidate. We analyze the mass density function of the distances in the CRM text-based fields and characterized their behavior and consistency in terms of the entropy and of the mutual information for these fields. Our experiments in a large CRM from a multinational hospitality chain show that the distance distributions are statistically consistent for each feature, and that neighbourhood thresholds are automatically adjusted by the system at a first step and they can be subsequently more-finely tuned according to the manager experience. The entropy distributions for the different variables, as well as the mutual information between pairs, are characterized by multimodal profiles, where a wide gap between close and far fields is often present. This motivates the proposal of the so-called X-from-M strategy, which is shown to be computationally affordable, and can provide the expert with a reduced number of duplicated candidates to supervise, with low X values being enough to warrant the sensitivity required at the automatic detection stage. The proposed system again encourages and supports the benefits of big-data technologies in CRM scenarios for hotel chains, and rather than the use of ad-hoc heuristic rules, it promotes the research and development of theoretically principled approaches.
The Einstein–Podolsky–Rosen Steering and Its Certification
Authors: Zhen Yi-Zheng , Xu Xin-Yu , Li Li , Liu Nai-Le , Chen Kai
Published: April 2019

The Einstein–Podolsky–Rosen (EPR) steering is a subtle intermediate correlation between entanglement and Bell nonlocality. It not only theoretically completes the whole picture of non-local effects but also practically inspires novel quantum protocols in specific scenarios. However, a verification of EPR steering is still challenging due to difficulties in bounding unsteerable correlations. In this survey, the basic framework to study the bipartite EPR steering is discussed, and general techniques to certify EPR steering correlations are reviewed.
Rolling 3D Laplacian Pyramid Video Fusion
Authors: Pavlović Rade , Petrović Vladimir
Published: April 2019

In this paper, we present a novel algorithm for video fusion of multi-sensor sequences applicable to real-time night vision systems. We employ the Laplacian pyramid fusion of a block of successive frames to add temporal robustness to the fused result. For the fusion rule, we first group high and low frequency levels of the decomposed frames in the block from both input sensor sequences. Then, we define local space-time energy measure to guide the selection based fusion process in a manner that achieves spatio-temporal stability. We demonstrate our approach on several well-known multi-sensor video fusion examples with varying contents and target appearance and show its advantage over conventional video fusion approaches. Computational complexity of the proposed methods is kept low by the use of simple linear filtering that can be easily parallelised for implementation on general-purpose graphics processing units (GPUs).
Autonomous Control of Unmanned Aerial Vehicles
Authors: Becerra Victor M.
Published: April 2019

Unmanned aerial vehicles (UAVs) are being increasingly used in different applications in both military and civilian domains [...]
Feasibility Study on Variable-Speed Air Conditioner under Hot Climate based on Real-Scale Experiment and Energy Simulation
Authors: Lim Jaehun , Yoon Myung Sup , Al-Qahtani Turki , Nam Yujin
Published: April 2019

It is well known that inverter-driven variable-speed compressor (or inverter) air conditioners are more efficient than constant-speed compressor air conditioners. Therefore, most countries have employed part-load assessment test standards such as ISO 16358, EN 14825 and ASHRAE 116 in addition to the conventional ISO 5151 full-load test standard to appropriately evaluate the part-load efficiencies of variable-speed air conditioners. However, many countries in the Middle East and South America still use the ISO 5151 standard owing to policy or high- temperature environmental considerations. In this study, we experimentally verify the energy saving effect of the inverter air conditioner with respect to the constant-speed air conditioner under the Korean climate with distinct temperature changes of four seasons and under the hot climate of Saudi Arabia throughout the year. ISO 5151 defines test conditions for a single temperature, whereas ISO 16358, EN 14825 and ASHRAE 116 simulate seasonal efficiencies using interpolation of several climate test results. Herein, we directly employ the environmental changes during a day or season in a qualified test room with specific dimension. Using extensive regional and seasonal climate data for Saudi Arabia and South Korea, the changes in temperature conditions are applied directly to the outdoor side and appropriate building cooling load conditions are applied to the indoor side of the air-enthalpy-type test room. The energy savings of the inverter air conditioner were analyzed experimentally according to the spatial and temporal temperature changes. The energy reduction effects of the inverter air conditioner largely depended on the temperature and cooling load changes for a day or season. Furthermore, a feasibility study based on an energy simulation showed that the variable-speed air conditioner could be economical even in hot climates.
Battery Storage Integration in Voltage Unbalance and Overvoltage Mitigation Control Strategies and Its Impact on the Power Quality
Authors: Bozalakov Dimitar , Mnati Mohannad J. , Laveyne Joannes , Desmet Jan , Vandevelde Lieven
Published: April 2019

The increased utilisation of distributed renewable energy sources in low voltage grids leads to power quality problems such as overvoltages and voltage unbalance. This imposes challenges to the distribution system operators to maintain the power quality in their grids. To overcome these issues, energy storage systems could be integrated together with the distributed energy resources and the stored energy could be used when needed to better improve power quality and achieve better grid performance. However, integrating an energy storage system introduces additional cost, therefore, determining the right capacity is essential. In this article, an energy storage system is combined with the classical positive-sequence control strategy and the three-phase damping control strategy. The three-phase damping control strategy is able to mitigate the voltage unbalance by emulating a resistive behaviour towards the zero- and negative-sequence voltage components. This resistive behaviour can be set on different values such that the desired voltage unbalance mitigation is achieved. Hence, the three-phase damping control strategy, equipped with the energy storage system is investigated under different values of the resistive behaviour. Both control strategies are investigated under the same conditions and the impact of the different capacities of the energy storage systems is investigated.
Wind Turbine Power Curve Upgrades: Part II
Authors: Astolfi Davide , Castellani Francesco
Published: April 2019

Wind turbine power upgrades have recently become a debated topic in wind energy research. Their assessment poses some challenges and calls for devoted techniques: some reasons are the stochastic nature of the wind and the multivariate dependency of wind turbine power. In this work, two test cases were studied. The former is the yaw management optimization on a 2 MW wind turbine; the latter is a comprehensive control upgrade (pitch, yaw, and cut-out) for 850 kW wind turbines. The upgrade impact was estimated by analyzing the difference between the post-upgrade power and a data-driven simulation of the power if the upgrade did not take place. Therefore, a reliable model for the pre-upgrade power of the wind turbines of interest was needed and, in this work, a principal component regression was employed. The yaw control optimization was shown to provide a 1.3% of production improvement and the control re-powering provided 2.5%. Another qualifying point was that, for the 850 kW wind turbine re-powering, the data quality was sufficient for an upgrade estimate based on power curve analysis and a good agreement with the model result was obtained. Summarizing, evidence of the profitability of wind turbine power upgrades was collected and data-driven methods were elaborated for power upgrade assessment and, in general, for wind turbine performance control and monitoring.
Performance of the Stator Winding Fault Diagnosis in Sensorless Induction Motor Drive
Authors: Tarchała Grzegorz , Wolkiewicz Marcin
Published: April 2019

This paper deals with the diagnosis of stator winding inter-turn faults for an induction motor drive operating without a speed sensor in a speed-sensorless mode. The rotor direct field oriented control structure (DFOC) was applied, its reference current and voltage component values were analyzed, and their selected harmonics were applied as effective fault indicators. To ensure robust speed estimation, a sliding mode model reference adaptive system (SM-MRAS) estimator was selected. The influence of load torque, reference speed, proportional-integral (PI) controller parameters, and short-circuit current on fault diagnosis and speed estimation performance was verified. Experimental test results obtained for a 3 kW induction motor drive are included.
Group Method of Data Handling (GMDH) Lithology Identification Based on Wavelet Analysis and Dimensionality Reduction as Well Log Data Pre-Processing Techniques
Authors: Shen Chuanbo , Asante-Okyere Solomon , Yevenyo Ziggah Yao , Wang Liang , Zhu Xiangfeng
Published: April 2019

Although the group method of data handling (GMDH) is a self-organizing metaheuristic neural network capable of developing a classification function using influential input variables, the results can be improved by using some pre-processing steps. In this paper, we propose a joint principal component analysis (PCA) and GMDH (PCA-GMDH) classifier method. We investigated well log data pre-processing techniques composed of dimensionality reduction (DR) and wavelet analysis (WA), using the southern basin of the South Yellow Sea as a case study, with the aim of improving the lithology classification accuracy of the GMDH. Our results showed that the dimensionality reduction method, which is composed of PCA and linear discriminant analysis (LDA), minimized the complexity of the classifier by reducing the number of well log suites to the relevant components and factors. On the other hand, the WA decomposed the well log signals into time-frequency wavelets for the GMDH algorithm. Of all the pre-processing methods, only the PCA was able to significantly increase the classification accuracy rate of the GMDH. Finally, the proposed joint PCA-GMDH classifier not only increased the accuracy but also was able to distinguish between all the classes of lithofacies present in the southern basin of the South Yellow Sea.
Analysis of the Spatial–Temporal Variation of the Surface Ozone Concentration and Its Associated Meteorological Factors in Changchun
Authors: Fang Chunsheng , Wang Liyuan , Wang Ju
Published: April 2019

Ozone (O3) pollution has become one of the most challenging problems in China, and high O3 concentrations have been a major air quality issue in Changchun. Based on continuous observation data of surface ozone concentrations from ten automatic air monitoring stations and meteorological data from the meteorological bureau in Changchun, the temporal and spatial variations of the O3 concentration and its relationships with meteorological factors were analyzed by correlation analysis during the period of 2013–2017. The results showed the following: A single apex model of the annual mean O3 concentrations of the daily maximum 8 h average (MDA8) was found from the data for 2013 to 2017 in Changchun, with the highest MDA8 O3 concentrations in 2015 and a slight decline from then until 2017. The O3 concentrations in the suburban areas and the south of Changchun were higher than those downtown and north of the city. The seasonal variation of O3 concentrations was obvious, following the order summer > spring > autumn > winter, which was similar to the results of neighboring cities and provinces in Changchun. The days on which O3 concentrations exceeded the standard were concentrated in summer and spring, and the total number of ozone excess days was 91 days; the maximum number of ozone excess days was in 2015. The O3 concentration exceeded the standard in Changchun mainly in March–August, and its monthly mean value curve showed a bimodal type in which the highest values appeared in May and July, while the lowest values appeared in December. The diurnal pattern of ozone showed a single peak mode, and the peak value usually appeared at 14:00–16:00 while the minimum value appeared at 07:00–08:00. O3 concentrations in Changchun and the six selected pollutants CO, NO, NO2, NOx, PM10, and PM2.5 were negatively correlated. Higher temperature is a necessary synoptic condition for ozone pollution in Changchun: when the temperature rose, O3 concentrations increased significantly; further, O3 concentrations were negatively correlated with relative humidity and atmospheric pressure and were positively correlated with temperature and solar radiation. The O3 concentrations were highest when the wind scale approached 14~20 km/h and the wind direction was S. Combined with the research results in the surrounding areas of Changchun, it is indicated that there may be an ozone contribution from south of Changchun through long-range pollution transport and tropospheric subsidence.
Distribution and Habitat Selection of Free-Ranging European Bison (Bison bonasus L.) in a Mosaic Landscape—A Lithuanian Case
Authors: Marozas Vitas , Kibiša Artūras , Brazaitis Gediminas , Jõgiste Kalev , Šimkevičius Kastytis , Bartkevičius Edmundas
Published: April 2019

In Lithuania, free-ranging European bison live sedentarily in the fragmented landscape of the central part of the country. The aim of this study was to analyze the distribution and habitat selection of European bison in a semi-isolated territory. Free-ranging European bison living sedentarily have formed six groups in the mosaic landscape of central Lithuania. A herd of 34 individuals of European bison entered into a new semi-isolated territory in 2016. During the snow-free season, we tracked the movements of the herd by fitting a global positioning system (GPS) collar to the leading cow. To evaluate the home range and habitat selection, we used ArcGIS software, Jacobs’ index, and chi-square testing to verify significant differences between proportions. The home range of European bison was largest in spring, decreased in summer, and increased again in autumn; this was associated with feeding and the food supply in agricultural lands. European bison spent more time in the forest, especially during the daytime, due to disturbance, but in summer time and at night, the bison did not avoid agricultural land. In the forests, European bison preferred clear-cut, small-leaved deciduous (aspen, grey alder) forests of middle and mature age, but they avoided broad-leaved deciduous (ash), coniferous, and young forests. The increased number of European bison caused damage to agricultural crops and increased conflict with farmers.
Heterodichogamy, Pollen Viability, and Seed Set in a Population of Polyploidy Cyclocarya Paliurus (Batal) Iljinskaja (Juglandaceae)
Authors: Mao Xia , Fu Xiang-Xiang , Huang Peng , Chen Xiao-Ling , Qu Yin-Quan
Published: April 2019

Research Highlights: Cyclocarya paliurus, native to the subtropical region of China, is a monoecious species with a heterodichogamous mating system. Its flowering phenology and low seed success characteristics differ from other typical heterodichogamous Juglandaceae species. This could be caused by the existence of polyploidy in the population. Background and Objectives: C. paliurus has been attracting more attention as a result of its medicinal value. To meet the needs for leaf harvest, cultivation expansion is required, but this is limited by a shortage of seeds. This study aims to profile the flowering phenology and the efficacy of pollen dispersal as well as elucidate on the mechanism of low seed success in the population. Materials and Methods: The flowering phenology pattern of C. paliurus was observed in a juvenile plantation containing 835 individuals of 53 families from 8 provenances at the individual (protandry, PA and protogyny, PG) and population levels for 5 consecutive years (2014–2018). Slides with a culture medium of 10% sucrose and 0.01% boric acid were used to estimate pollen density and viability in the population, and seeds were collected from 20 randomly selected PA and PG individuals to assess seed success during 2017–2018. Results: Four flowering phenotypes and strongly skewed ratios of PA/PG and male/female occurred in the juvenile population. Sexual type and ratio changed significantly with the growth of the population over the years, showing an increasing monoecious group (11.1% to 57.2%) and a decreasing unisexual group (33.6% to 16.3%), as well as a tendency for the sexual ratio to move towards equilibrium (5.42:1 to 1.39:1 for PG:PA). Two flowering phases and bimodality in gender were displayed, as in other heterodichogamous species. However, the high overlap of inter-phases and within individuals was quite different from many previous reports. Owing to the low pollen viability of C. paliurus (~30%), low seed success was monitored in the plantation, as well as in the investigated natural populations. Conclusions: Female-bias (PG and F) and a skewed ratio of mating types corresponded to nutrient accumulation in the juvenile population. Heterodichogamy in C. paliurus was verified, but was shown to be different from other documented species in Juglandaceae. The latest finding of major tetraploidy in a natural population could explain the characteristics of the flowering phenology and seed set of C. paliurus and also give rise to more questions to be answered.
Can Field Crews Telecommute? Varied Data Quality from Citizen Science Tree Inventories Conducted Using Street-Level Imagery
Authors: Berland Adam , Roman Lara A. , Vogt Jess
Published: April 2019

Street tree inventories are a critical component of urban forest management. However, inventories conducted in the field by trained professionals are expensive and time-consuming. Inventories relying on citizen scientists or virtual surveys conducted remotely using street-level photographs may greatly reduce the costs of street tree inventories, but there are fundamental uncertainties regarding the level of data quality that can be expected from these emerging approaches to data collection. We asked 16 volunteers to inventory street trees in suburban Chicago using Google Street ViewTM imagery, and we assessed data quality by comparing their virtual survey data to field data from the same locations. We also compared virtual survey data quality according to self-rated expertise by measuring agreement within expert, intermediate, and novice analyst groups. Analyst agreement was very good for the number of trees on each street segment, and agreement was markedly lower for tree diameter class and tree identification at the genus and species levels, respectively. Interrater agreement varied by expertise, such that experts agreed with one another more often than novices for all four variables assessed. Compared to the field data, we observed substantial variability in analyst performance for diameter class estimation and tree identification, and some intermediate analysts performed as well as experts. Our findings suggest that virtual surveys may be useful for documenting the locations of street trees within a city more efficiently than field crews and with a high level of accuracy. However, tree diameter and species identification data were less reliable across all expertise groups, and especially novice analysts. Based on this analysis, virtual street tree inventories are best suited to collecting very basic information such as tree locations, or updating existing inventories to determine where trees have been planted or removed. We conclude with evidence-based recommendations for effective implementation of this type of approach.
Stability of an Axisymmetric Liquid Metal Flow Driven by a Multi-Pole Rotating Magnetic Field
Authors: Tagawa Toshio , Song Kewei
Published: April 2019

The stability of an electrically conducting fluid flow in a cylinder driven by a multi-pole rotating magnetic field is numerically studied. A time-averaged Lorentz force term including the electric potential is derived on the condition that the skin effect can be neglected and then it is incorporated into the Navier-Stokes equation as a body force term. The axisymmetric velocity profile of the basic flow for the case of an infinitely long cylinder depends on the number of pole-pairs and the Hartmann number. A set of linearized disturbance equations to obtain a neutral state was successfully solved using the highly simplified marker and cell (HSMAC) method together with a Newton–Raphson method. For various cases of the basic flow, depending on both the number of pole-pairs and the Hartmann number, the corresponding critical rotational Reynolds numbers for the onset of secondary flow were obtained instead of using the conventional magnetic Taylor number. The linear stability analyses reveal that the critical Reynolds number takes its minimum at a certain value of the Hartmann number. On the other hand, the velocity profile for cases of a finite length cylinder having a no-slip condition at the flat walls generates the Bödewadt boundary layers and such flows need to be computed including the non-linear terms of the Navier-Stokes equation.
History of Management and Stratigraphy of Organizing. The Venice Arsenal between Tangible and Intangible Heritage
Authors: Zan Luca
Published: April 2019

The purpose of this contribution is to analyze the role of the Arsenal in the history of management, still underrepresented in international historiography, and link it to the literature on industrial heritage. It is this central role in the history of management that endows the Venice Arsenal with particular significance as intangible heritage. Discussing specific problems related to the research and interpretation of this intangible significance, in a context of stratification of levels of organization over time, this paper considers some implications for restoration of the Arsenal, as well as area to preserve this intangible heritage in terms of research, documentation, and presentation needs.
Data Collection Instruments for Obesogenic Environments in Adults: A Scoping Review
Authors: Martínez-García Alba , Trescastro-López Eva María , Galiana-Sánchez María Eugenia , Pereyra-Zamora Pamela
Published: April 2019

The rise in obesity prevalence has increased research interest in the obesogenic environment and its influence on excess weight. The aim of the present study was to review and map data collection instruments for obesogenic environments in adults in order to provide an overview of the existing evidence and enable comparisons. Through the scoping review method, different databases and webpages were searched between January 1997 and May 2018. Instruments were included if they targeted adults. The documents were categorised as food environment or built environment. In terms of results, 92 instruments were found: 46 instruments measuring the food environment, 42 measuring the built environment, and 4 that characterised both environments. Numerous diverse instruments have been developed to characterise the obesogenic environment, and some of them have been developed based on existing ones; however, most of them have not been validated and there is very little similarity between them, hindering comparison of the results obtained. In addition, most of them were developed and used in the United States and were written in English. In conclusion, there is a need for a robust instrument, improving or combining existing ones, for use within and across countries, and more sophisticated study designs where the environment is contemplated in an interdisciplinary approach.
Sedentary Behaviour and 12 Sleep Problem Indicators among Middle-Aged and Elderly Adults in South Africa
Authors: Pengpid Supa , Peltzer Karl
Published: April 2019

The aim of this investigation was to assess the association of sedentary behaviour with 12 different sleep problem indicators among rural middle-aged and elderly adults in South Africa. Cross-sectional data were analysed from the “Health and Aging in Africa: A Longitudinal Study of an INDEPTH community in South Africa” (HAALSI) baseline survey. Participants responded to a questionnaire, including sociodemographic, health, anthropometric measures, sedentary behaviour and 12 different sleep problem indicators. The sample included 4782 individuals 40 years and older (median 61 years, interquartile range = 20 years). Overall, participants engaged <4 h (55.9%), 4–<8 h (34.1%), 8 or more hours a day (9.9%) sedentary time a day. In adjusted multinomial logistic regression, 8 h of more sedentary time was associated with short and long sleep. In adjusted logistic regression analysis, high sedentary time was positively associated with snoring, gasping, breathing stops and restless sleep and negatively associated with insufficient sleep and sleep problems due to a traumatic event. In combined analysis, compared to persons with low or moderate sedentary behaviour and moderate or high physical activity, persons with high sedentary behaviour and low physical activity were more likely to have long sleep, insufficient sleep, snoring, gasping, breathing stops, and restless sleep and less likely to have sleep problems due to traumatic events. Findings show an association between sedentary behaviour and/or combined sedentary behaviour and low physical activity with seven of 12 sleep problem indicators (short sleep, long sleep, insufficient sleep, snoring, gasping, breathing stops, and restless sleep).
Fluoride Exposure Induces Inhibition of Sodium-and Potassium-Activated Adenosine Triphosphatase (Na+, K+-ATPase) Enzyme Activity: Molecular Mechanisms and Implications for Public Health
Authors: Waugh Declan Timothy
Published: April 2019

In this study, several lines of evidence are provided to show that Na + , K + -ATPase activity exerts vital roles in normal brain development and function and that loss of enzyme activity is implicated in neurodevelopmental, neuropsychiatric and neurodegenerative disorders, as well as increased risk of cancer, metabolic, pulmonary and cardiovascular disease. Evidence is presented to show that fluoride (F) inhibits Na + , K + -ATPase activity by altering biological pathways through modifying the expression of genes and the activity of glycolytic enzymes, metalloenzymes, hormones, proteins, neuropeptides and cytokines, as well as biological interface interactions that rely on the bioavailability of chemical elements magnesium and manganese to modulate ATP and Na + , K + -ATPase enzyme activity. Taken together, the findings of this study provide unprecedented insights into the molecular mechanisms and biological pathways by which F inhibits Na + , K + -ATPase activity and contributes to the etiology and pathophysiology of diseases associated with impairment of this essential enzyme. Moreover, the findings of this study further suggest that there are windows of susceptibility over the life course where chronic F exposure in pregnancy and early infancy may impair Na + , K + -ATPase activity with both short- and long-term implications for disease and inequalities in health. These findings would warrant considerable attention and potential intervention, not to mention additional research on the potential effects of F intake in contributing to chronic disease.
Factors Influencing the Response to Infectious Diseases: Focusing on the Case of SARS and MERS in South Korea
Authors: Lee Kyu-Myoung , Jung Kyujin
Published: April 2019

Following the 2003 the severe acute respiratory syndrome (SARS) and the 2015 Middle East Respiratory Syndrome (MERS) outbreak in South Korea, this research aims to explore and examine the factors influencing the response to infectious diseases, which encompasses both communicable and non-communicable diseases. Through a qualitative research method, this research categorizes the factors as inputs, processes and outputs and applies them into the 2003 SARS and MERS outbreak in South Korea. As the results conducted meta-analyses to comprehensively analyze the correlations of factors influencing disaster response from a Korean context, the findings show that the legislative factor had direct and indirect influence on the overall process of infectious disease response and that Leadership of the central government, establishment of an intergovernmental response system, the need for communication, information sharing and disclosure and onsite response were identified as key factors influencing effective infectious disease response.
Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3
Authors: Ronca Shannon E. , Gorchakov Rodion , Berry Rebecca , Alvarado R. Elias , Gunter Sarah M. , Murray Kristy O.
Published: April 2019

West Nile virus (WNV) is an arbovirus with important public health implications globally. This study characterizes a viral isolate, 2004Hou3, in comparison with the NY99 strain from the original WNV outbreak in New York, USA. NextGen sequencing was used to compare the viral isolates genetically, while wild-type C57/BL6 mice were used to compare pathogenicity and viral persistence. Significant differences in survival and clinical presentations were noted, with minor genetic variations between the two strains potentially offering an explanation. One notable difference is that 5 of 35 mice infected with the 2004Hou3 strain developed hind limb flaccid paralysis, suggesting its possible use as a small animal pathogenesis model for this clinical characteristic often observed in human WN neuroinvasive disease patients but not reported in other animal models of infection. Overall, this study suggests that 2004Hou3 is a less pathogenic strain with potential for use in long-term outcome studies using small animal models.
How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake
Authors: Henaux Loïc , Thibodeau Jacinthe , Pilon Geneviève , Gill Tom , Marette André , Bazinet Laurent
Published: April 2019

The valorization of by-products from natural organic sources is an international priority to respond to environmental and economic challenges. In this context, electrodialysis with filtration membrane (EDFM), a green and ultra-selective process, was used to separate peptides from salmon frame protein hydrolysate. For the first time, the simultaneous separation of peptides by three ultrafiltration membranes of different molecular-weight exclusion limits (50, 20, and 5 kDa) stacked in an electrodialysis system, allowed for the generation of specific cationic and anionic fractions with different molecular weight profiles and bioactivity responses. Significant decreases in peptide recovery, yield, and molecular weight (MW) range were observed in the recovery compartments depending on whether peptides had to cross one, two, or three ultrafiltration membranes. Moreover, the Cationic Recovery Compartment 1 fraction demonstrated the highest increase (42%) in glucose uptake on L6 muscle cells. While, in the anionic configuration, both Anionic Recovery Compartment 2 and Anionic Recovery Compartment 3 fractions presented a glucose uptake response in basal condition similar to the insulin control. Furthermore, Cationic Recovery Compartment 3 was found to contain inhibitory peptides. Finally, LC-MS analyses of the bioassay-guided bioactive fractions allowed us to identify 11 peptides from salmon by-products that are potentially responsible for the glucose uptake improvement.
PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803
Authors: Koch Moritz , Doello Sofía , Gutekunst Kirstin , Forchhammer Karl
Published: April 2019

Polyhydroxybutyrate (PHB) is a polymer of great interest as a substitute for conventional plastics, which are becoming an enormous environmental problem. PHB can be produced directly from CO2 in photoautotrophic cyanobacteria. The model cyanobacterium Synechocystis sp. PCC 6803 produces PHB under conditions of nitrogen starvation. However, it is so far unclear which metabolic pathways provide the precursor molecules for PHB synthesis during nitrogen starvation. In this study, we investigated if PHB could be derived from the main intracellular carbon pool, glycogen. A mutant of the major glycogen phosphorylase, GlgP2 (slr1367 product), was almost completely impaired in PHB synthesis. Conversely, in the absence of glycogen synthase GlgA1 (sll0945 product), cells not only produced less PHB, but were also impaired in acclimation to nitrogen depletion. To analyze the role of the various carbon catabolic pathways (EMP, ED and OPP pathways) for PHB production, mutants of key enzymes of these pathways were analyzed, showing different impact on PHB synthesis. Together, this study clearly indicates that PHB in glycogen-producing Synechocystis sp. PCC 6803 cells is produced from this carbon-pool during nitrogen starvation periods. This knowledge can be used for metabolic engineering to get closer to the overall goal of a sustainable, carbon-neutral bioplastic production.
Acute Effects of Single Doses of Bonito Fish Peptides and Vitamin D on Whole Blood Gene Expression Levels: A Randomized Controlled Trial
Authors: Guénard Frédéric , Jacques Hélène , Gagnon Claudia , Marette André , Vohl Marie-Claude
Published: April 2019

Fish contains high quality proteins and essential nutrients including 25-hydroxyvitamin D (25(OH)D). Fish peptide consumption can lower cardiovascular disease (CVD) risk factors, and studies have shown an association between 25(OH)D deficiency, CVD and CVD risk factors, such as diabetes. This study investigated acute effects of a single dose of cholecalciferol (VitD3), bonito fish peptide hydrolysate (BPH), or a combination of both on CVD risk factors and whole blood gene expression levels. A randomized, crossover, placebo controlled trial was conducted in 22 adults. They ingested, in random order and at 7-day intervals, 1000 IU of VitD3, 3 g of BPH, a combination of both, or a placebo. A 180 min oral glucose tolerance test was performed. Differences in whole-genome expression levels after versus before each supplementation were computed for 18 subjects. We observed that 16, 1 and 5 transcripts were differentially expressed post- vs. pre-ingestion for VitD3, BPH or VitD3 + BPH treatments, respectively. VitD3-containing treatments affected the expression of the solute carrier family 25 member 20 (SLC25A20) gene involved in fatty acid oxidation, various transcription factors and genes related to glucose metabolism. These results suggest that VitD3 rapidly modulates genes related to CVD risk factors in blood while BPH seems to moderately modulate gene expression levels.
Disruption of Structural Disulfides of Coagulation FXIII-B Subunit; Functional Implications for a Rare Bleeding Disorder
Authors: Singh Sneha , Akhter Mohammad Suhail , Dodt Johannes , Sharma Amit , Kaniyappan Senthilvelrajan , Yadegari Hamideh , Ivaskevicius Vytautas , Oldenburg Johannes , Biswas Arijit
Published: April 2019

Congenital FXIII deficiency is a rare bleeding disorder in which mutations are detected in F13A1 and F13B genes that express the two subunits of coagulation FXIII, the catalytic FXIII-A, and protective FXIII-B. Mutations in FXIII-B subunit are considerably rarer compared to FXIII-A. Three mutations in the F13B gene have been reported on its structural disulfide bonds. In the present study, we investigate the structural and functional importance of all 20 structural disulfide bonds in FXIII-B subunit. All disulfide bonds were ablated by individually mutating one of its contributory cysteine’s, and these variants were transiently expressed in HEK293t cell lines. The expression products were studied for stability, secretion, the effect on oligomeric state, and on FXIII-A activation. The structural flexibility of these disulfide bonds was studied using classical MD simulation performed on a FXIII-B subunit monomer model. All 20 FXIII-B were found to be important for the secretion and stability of the protein since ablation of any of these led to a secretion deficit. However, the degree of effect that the disruption of disulfide bond had on the protein differed between individual disulfide bonds reflecting a functional hierarchy/diversity within these disulfide bonds.
Construction of Dimeric Drug-Loaded Polymeric Micelles with High Loading Efficiency for Cancer Therapy
Authors: Yu Bing , Meng Qingye , Hu Hao , Xu Tao , Shen Youqing , Cong Hailin
Published: April 2019

Polymeric micelles (PMs) have been applied widely to transport hydrophobic drugs to tumor sites for cancer treatment. However, the low load efficiency of the drug in the PMs significantly reduces the therapeutic efficiency. We report here that disulfide-linked camptothecin (CPT) as a kind of dimeric drug can be effectively embedded in the core of poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCL–PEG–PCL) PMs for improving drug-loading efficiency, and PEG can be used as a hydrophilic shell. Moreover, the dimeric CPT-loaded PCL–PEG–PCL PMs exhibited excellent solubility in phosphate-buffered saline (PBS) media and significant cytotoxicity to cancer cells.
The Pathway to Cancer Cachexia: MicroRNA-Regulated Networks in Muscle Wasting Based on Integrative Meta-Analysis
Authors: Freire Paula Paccielli , Fernandez Geysson Javier , Cury Sarah Santiloni , de Moraes Diogo , Oliveira Jakeline Santos , de Oliveira Grasieli , Dal-Pai-Silva Maeli , dos Reis Patrícia Pintor , Carvalho Robson Francisco
Published: April 2019

Cancer cachexia is a multifactorial syndrome that leads to significant weight loss. Cachexia affects 50%–80% of cancer patients, depending on the tumor type, and is associated with 20%–40% of cancer patient deaths. Besides the efforts to identify molecular mechanisms of skeletal muscle atrophy—a key feature in cancer cachexia—no effective therapy for the syndrome is currently available. MicroRNAs are regulators of gene expression, with therapeutic potential in several muscle wasting disorders. We performed a meta-analysis of previously published gene expression data to reveal new potential microRNA–mRNA networks associated with muscle atrophy in cancer cachexia. We retrieved 52 differentially expressed genes in nine studies of muscle tissue from patients and rodent models of cancer cachexia. Next, we predicted microRNAs targeting these differentially expressed genes. We also include global microRNA expression data surveyed in atrophying skeletal muscles from previous studies as background information. We identified deregulated genes involved in the regulation of apoptosis, muscle hypertrophy, catabolism, and acute phase response. We further predicted new microRNA–mRNA interactions, such as miR-27a/Foxo1, miR-27a/Mef2c, miR-27b/Cxcl12, miR-27b/Mef2c, miR-140/Cxcl12, miR-199a/Cav1, and miR-199a/Junb, which may contribute to muscle wasting in cancer cachexia. Finally, we found drugs targeting MSTN, CXCL12, and CAMK2B, which may be considered for the development of novel therapeutic strategies for cancer cachexia. Our study has broadened the knowledge of microRNA-regulated networks that are likely associated with muscle atrophy in cancer cachexia, pointing to their involvement as potential targets for novel therapeutic strategies.
RNA-Binding Proteins HuB, HuC, and HuD are Distinctly Regulated in Dorsal Root Ganglia Neurons from STZ-Sensitive Compared to STZ-Resistant Diabetic Mice
Authors: Mustăciosu Cosmin Cătălin , Banciu Adela , Rusu Călin Mircea , Banciu Daniel Dumitru , Savu Diana , Radu Mihai , Radu Beatrice Mihaela
Published: April 2019

The neuron-specific Elav-like Hu RNA-binding proteins were described to play an important role in neuronal differentiation and plasticity by ensuring the post-transcriptional control of RNAs encoding for various proteins. Although Elav-like Hu proteins alterations were reported in diabetes or neuropathy, little is known about the regulation of neuron-specific Elav-like Hu RNA-binding proteins in sensory neurons of dorsal root ganglia (DRG) due to the diabetic condition. The goal of our study was to analyze the gene and protein expression of HuB, HuC, and HuD in DRG sensory neurons in diabetes. The diabetic condition was induced in CD-1 adult male mice with single-intraperitoneal injection of streptozotocin (STZ, 150 mg/kg), and 8-weeks (advanced diabetes) after induction was quantified the Elav-like proteins expression. Based on the glycemia values, we identified two types of responses to STZ, and mice were classified in STZ-resistant (diabetic resistant, glycemia < 260 mg/dL) and STZ-sensitive (diabetic, glycemia > 260 mg/dL). Body weight measurements indicated that 8-weeks after STZ-induction of diabetes, control mice have a higher increase in body weight compared to the diabetic and diabetic resistant mice. Moreover, after 8-weeks, diabetic mice (19.52 ± 3.52 s) have longer paw withdrawal latencies in the hot-plate test than diabetic resistant (11.36 ± 1.92 s) and control (11.03 ± 1.97 s) mice, that correlates with the installation of warm hypoalgesia due to the diabetic condition. Further on, we evidenced the decrease of Elav-like gene expression in DRG neurons of diabetic mice (Elavl2, 0.68 ± 0.05 fold; Elavl3, 0.65 ± 0.01 fold; Elavl4, 0.53 ± 0.07 fold) and diabetic resistant mice (Ealvl2, 0.56 ± 0.07 fold; Elavl3, 0.32 ± 0.09 fold) compared to control mice. Interestingly, Elav-like genes have a more accentuated downregulation in diabetic resistant than in diabetic mice, although hypoalgesia was evidenced only in diabetic mice. The Elav-like gene expression changes do not always correlate with the Hu protein expression changes. To detail, HuB is upregulated and HuD is downregulated in diabetic mice, while HuB, HuC, and HuD are downregulated in diabetic resistant mice compared to control mice. To resume, we demonstrated HuD downregulation and HuB upregulation in DRG sensory neurons induced by diabetes, which might be correlated with altered post-transcriptional control of RNAs involved in the regulation of thermal hypoalgesia condition caused by the advanced diabetic neuropathy.
UBIAD1 Plays an Essential Role in the Survival of Pancreatic Acinar Cells
Authors: Nakagawa Kimie , Fujiwara Kiyomi , Nishimura Akihiro , Murakami Chinami , Kawamoto Kanaha , Ichinose Chihiro , Kunitou Yumi , Suhara Yoshitomo , Okano Toshio , Hasegawa Hiroshi
Published: April 2019

UbiA prenyltransferase domain-containing protein 1 (UBIAD1) is a vitamin K2 biosynthetic enzyme. We previously showed the lethality of this enzyme in UBIAD1 knockout mice during the embryonic stage. However, the biological effects of UBIAD1 deficiency after birth remain unclear. In the present study, we used a tamoxifen-inducible systemic UBIAD1 knockout mouse model to determine the role of UBIAD1 in adult mice. UBIAD1 knockout resulted in the death of the mice within about 60 days of administration of tamoxifen. The pancreas presented with the most prominent abnormality in the tamoxifen-induced UBIAD1 knockout mice. The pancreas was reduced remarkably in size; furthermore, the pancreatic acinar cells disappeared and were replaced by vacuoles. Further analysis revealed that the vacuoles were adipocytes. UBIAD1 deficiency in the pancreatic acinar cells caused an increase in oxidative stress and autophagy, leading to apoptotic cell death in the tamoxifen-induced UBIAD 1 knockout mice. These results indicate that UBIAD1 is essential for maintaining the survival of pancreatic acinar cells in the pancreas.
Exposure to Insecticides Reduces Populations of Rhynchophorus palmarum in Oil Palm Plantations with Bud Rot Disease
Authors: Martínez Luis Carlos , Plata-Rueda Angelica , Rodríguez-Dimaté Francisco Andrés , Campos Juliana Mendonça , Santos Júnior Valdeir Celestino dos , Rolim Gabriela Da Silva , Fernandes Flavio Lemes , Silva Wiane Meloni , Wilcken Carlos Frederico , Zanuncio José Cola , Serrão José Eduardo
Published: April 2019

The South American palm weevil (SAPW), Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) is the main pest of Elaeis guineensis and damages palm trees with bud rot disease in the Americas. The effects of six neurotoxic insecticides (abamectin, carbaryl, deltamethrin, fipronil, imidacloprid and spinosad) were evaluated against SAPW for toxicity, survival, reproduction, and mortality. Abamectin (LC50 = 0.33 mg mL−1), Carbaryl (LC50 = 0.24 mg mL−1), deltamethrin (LC50 = 0.17 mg mL−1), and fipronil (LC50 = 0.42 mg mL−1) were the most toxic to SAPW. Adult survival was 95% without exposure to insecticides, decreasing to 78–65% in insects treated with the LC25 and 49–35% in insects exposed to LC50. Sublethal doses of carbaryl, fipronil and imidacloprid showed significant effect on the reproduction of this insect. Mortality of SAPW populations caused by insecticides had similar effects in the laboratory and field conditions. The results suggest that carbaryl, deltamethrin, fipronil, and imidacloprid caused significantly higher mortality as compared to the control in SAPW and may be used to control its populations in oil palm trees where bud rot appears as the key disease for SAPW attraction and infestation.