bluuebunny/crossref_metadata_2025 huggingface dataset:
Deciphering the M-cell niche: insights from mouse models on how microfold cells “know” where they are needed
Authors: Del Castillo Diana , Lo David D.
Published: May 2024

Known for their distinct antigen-sampling abilities, microfold cells, or M cells, have been well characterized in the gut and other mucosa including the lungs and nasal-associated lymphoid tissue (NALT). More recently, however, they have been identified in tissues where they were not initially suspected to reside, which raises the following question: what external and internal factors dictate differentiation toward this specific role? In this discussion, we will focus on murine studies to determine how these cells are identified (e.g., markers and function) and ask the broader question of factors triggering M-cell localization and patterning. Then, through the consideration of unconventional M cells, which include villous M cells, Type II taste cells, and medullary thymic epithelial M cells (microfold mTECs), we will establish the M cell as not just a player in mucosal immunity but as a versatile niche cell that adapts to its home tissue. To this end, we will consider the lymphoid structure relationship and apical stimuli to better discuss how the differing cellular programming and the physical environment within each tissue yield these cells and their unique organization. Thus, by exploring this constellation of M cells, we hope to better understand the multifaceted nature of this cell in its different anatomical locales.
Anti-NMDAR encephalitis in a child with long impaired consciousness and persistent antibodies: a case report and mini review
Authors: Zhang Wenhao , Cao Wenjia , Tao Wenhan , Wang Yufei , Tangzhu Chenchen , Shen Qinru , Shi Xulai
Published: May 2024

We described a challenging case of anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis in a young girl. Despite enduring months of reduced consciousness with ongoing antibody presence, she ultimately exhibited remarkable improvement within a 5-year follow-up period. Additionally, we conducted a concise review of relevant literature on anti-NMDAR encephalitis, with a specific focus on anti-NMDAR antibodies. Our findings enhance the clinical comprehension of anti-NMDAR encephalitis and offer valuable insights to clinicians for its management.
Advancements in ovarian cancer immunodiagnostics and therapeutics via phage display technology
Authors: Li Yang , Li Xiao-meng , Yang Kai-di , Tong Wei-hua
Published: May 2024

Ovarian cancer, ranking as the seventh most prevalent malignancy among women globally, faces significant challenges in diagnosis and therapeutic intervention. The difficulties in early detection are amplified by the limitations and inefficacies inherent in current screening methodologies, highlighting a pressing need for more efficacious diagnostic and treatment strategies. Phage display technology emerges as a pivotal innovation in this context, utilizing extensive phage-peptide libraries to identify ligands with specificity for cancer cell markers, thus enabling precision-targeted therapeutic strategies. This technology promises a paradigm shift in ovarian cancer management, concentrating on targeted drug delivery systems to improve treatment accuracy and efficacy while minimizing adverse effects. Through a meticulous review, this paper evaluates the revolutionary potential of phage display in enhancing ovarian cancer therapy, representing a significant advancement in combating this challenging disease. Phage display technology is heralded as an essential instrument for developing effective immunodiagnostic and therapeutic approaches in ovarian cancer, facilitating early detection, precision-targeted medication, and the implementation of customized treatment plans.
A learnable full-frequency transformer dual generative adversarial network for underwater image enhancement
Authors: Zheng Shijian , Wang Rujing , Zheng Shitao , Wang Liusan , Liu Zhigui
Published: May 2024

Underwater applications present unique challenges such as color deviation, noise, and low contrast, which can degrade image quality. Addressing these issues, we propose a novel approach called the learnable full-frequency transformer dual generative adversarial network (LFT-DGAN). Our method comprises several key innovations. Firstly, we introduce a reversible convolution-based image decomposition technique. This method effectively separates underwater image information into low-, medium-, and high-frequency domains, enabling more thorough feature extraction. Secondly, we employ image channels and spatial similarity to construct a learnable full-frequency domain transformer. This transformer facilitates interaction between different branches of information, enhancing the overall image processing capabilities. Finally, we develop a robust dual-domain discriminator capable of learning spatial and frequency domain characteristics of underwater images. Extensive experimentation demonstrates the superiority of the LFT-DGAN method over state-of-the-art techniques across multiple underwater datasets. Our approach achieves significantly improved quality and evaluation metrics, showcasing its effectiveness in addressing the challenges posed by underwater imaging. The code can be found at https://github.com/zhengshijian1993/LFT-DGAN.
Spatiotemporal structure of foraging and path integration errors by fiddler crabs, Leptuca pugilator
Authors: Chatterji Ruma , Layne John E.
Published: May 2024

Path integration is the navigational process by which animals construct a memory of a previous location by continuously measuring and summing their movements to form a single home vector pointing to the starting location. It is intrinsically error prone, subject to random errors and, potentially, to systematic errors in either measurement or the summing algorithm. Both types of errors lead to an incorrect vector memory and thus to an error in homing. Because the errors are incurred when animals move, they are theoretically predictable from the movements. We analyzed the behavior of fiddler crabs (Leptuca pugilator) as they performed foraging excursions followed by homing with varying degrees of error. From video recordings we measured body orientations and locations and computed these spatiotemporal path characteristics: duration, distance, turns, bearing and arc sector. These were analyzed for their effect on, separately, the magnitude, and the direction, of crabs’ homing error. The magnitude of the homing error was predicted by arc sector, Δbearing and path length, and several interactions. The direction of the homing error was predicted by interactions including arc sector x Δbearing, arc sector x turns, and Δbearing x turns. Covariance among these factors results in a path that traces a large arc while maintaining body orientation toward the burrow direction and leads to an error with the same clockwise/counterclockwise sign as the arc and the body turns. These results place L. pugilator’s path integration mechanism among others with known systematic errors.
Empowering health professions educators: enhancing curriculum delivery through customized e-tutorial training on fundamental digital tools
Authors: Sadiq Naushaba , Fatima Syeda Hanaa , Shabnam Nadia , Rauf Ayesha
Published: May 2024

Introduction

In the dynamic landscape of education, the fusion of technology and learning, commonly termed “technology-enhanced learning” (TEL), has emerged as a transformative phenomenon. This study focuses on the imperative integration of TEL in medical education, recognizing the diverse digital literacy levels of adult learners. The exploration introduces the innovative E-Portal training program, designed to empower health professions educators with essential skills for proficiently employing digital tools in instruction.

Methodology

A dedicated team of medical educationists conducted a thorough investigation into E-curriculum design and delivery, employing the Moodle Learning Management System as the foundation for the E-Portal training program. The training, spanning crucial stages such as course design, content delivery, self-paced teaching, and quality assessment, facilitated participant progression at individual paces, unlocking subsequent steps upon meeting stipulated prerequisites. A pre-training questionnaire gauged participants’ comprehension of distance learning, e-learning, synchronous and asynchronous teaching, and self-directed study. Subsequent focus group discussion post-training generated rich insights into participants’ experiences, reflections, and identified challenges.

Results

The results illuminate participants’ limited adeptness with e-learning terminology, successful assimilation of components and functionalities, and heightened confidence in online teaching practices. However, discerned challenges during implementation, such as technical hurdles and engagement issues, highlight the multifaceted nature of TEL integration. While the E-Portal training positively impacted preparedness, participant feedback advocates for improvements in assessment tools, technical training provisions, and exploration of alternative Learning Management Systems.

Discussion and conclusion

Study emphasizes the ongoing need for diverse training methodologies across Learning Management Systems, acknowledging the evolving nature of education and technology. This study underscores the transformative role of a tailored E-Portal training program in seamlessly integrating digital tools into medical education. The comprehensive insights provided contribute to a nuanced understanding of the advantages, obstacles, and potential avenues for enhancement in curriculum delivery through technology-enhanced learning, thereby propelling the field forward.
Microbial dysbiosis in systemic lupus erythematosus: a scientometric study
Authors: Zhao Miaomiao , Wen Xiaoting , Liu Ruiling , Xu Ke
Published: May 2024

Introduction

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. Mounting evidence suggests microbiota dysbiosis augment autoimmune response. This study aims to provide a systematic overview of this research field in SLE through a bibliometric analysis.

Methods

We conducted a comprehensive search and retrieval of literature related to microbial researches in SLE from the Web of Science Core Collection (WOSCC) database. The retrieved articles were subjected to bibliometric analysis using VOSviewer and Bibliometricx to explore annual publication output, collaborative patterns, research hotspots, current research status, and emerging trends.

Results

In this study, we conducted a comprehensive analysis of 218 research articles and 118 review articles. The quantity of publications rises annually, notably surging in 2015 and 2018. The United States and China emerged as the leading contributors in microbial research of SLE. Mashhad University of Medical Sciences had the highest publication outputs among the institutions. Frontiers in Immunology published the most papers. Luo XM and Margolles A were the most prolific and highly cited contributors among individual authors. Microbial research in SLE primarily focused on changes in microbial composition, particularly gut microbiota, as well as the mechanisms and practical applications in SLE. Recent trends emphasize “metabolites,” “metabolomics,” “fatty acids,” “T cells,” “lactobacillus,” and “dietary supplementation,” indicating a growing emphasis on microbial metabolism and interventions in SLE.

Conclusion

This study provides a thorough analysis of the research landscape concerning microbiota in SLE. The microbial research in SLE mainly focused on three aspects: microbial dysbiosis, mechanism studies and translational studies (microbiota-based therapeutics). It identifies current research trends and focal points, offering valuable guidance for scholars in the field.
Variations in different preceding crops on the soil environment, bacterial community richness and diversity of tobacco-planting soil
Authors: Liu Ming , Xue Rujun , Wang Dexun , Hu Yanxia , Gu Kaiyuan , Yang Liu , Zhao Jie , Guan Shuyue , Su Jiaen , Jiang Yonglei
Published: May 2024

Tobacco (Nicotiana tabacum L.) is a major cash crop, and soil quality played a significant role in the yield and quality of tobacco. Most farmers cultivate tobacco in rotation with other crops to improve the soil characteristics. However, the effects of different previous crops on the soil’s nutrient status and bacterial community for tobacco cultivation still need to be determined. Three treatments were assessed in this study, i.e., tobacco-planting soil without treatment (CK), soil with barley previously cultivated (T1), and soil with rapeseed previously cultivated (T2). The soil physical and chemical properties and the 16S rRNA gene sequence diversity of the bacterial community were analyzed. The effects of different crops on the physical and chemical properties of tobacco-planting soil and the diversity and richness of the bacterial community were comprehensively discussed. The results of this study showed that different previously cultivated crops altered the nutrient status of the soil, with changes in the ratio of NH4+-N to NO3−-N having the most significant impact on tobacco. In CK, the ratio of NH4+-N to NO3−-N was 1:24.2, T1–1:9.59, and T2–1:11.10. The composition of the bacterial community in tobacco-planting soil varied significantly depending on the previously cultivated crops. The richness and diversity of the bacterial community with different crops were considerably higher than without prior cultivation of different crops. The dominant bacteria in different treatments were Actinobacteriota, Proteobacteria, and Chloroflexi with their relative abundance differed. In conclusion, our study revealed significant differences in nutrient status, bacterial community diversity, and the richness of tobacco-planting soil after the preceding cultivation of different crops. Suitable crops should be selected to be previously cultivated in tobacco crop rotations in near future for sustainable agriculture.
Xylella phage MATE 2: a novel bacteriophage with potent lytic activity against Xylella fastidiosa subsp. pauca
Authors: Sabri Miloud , El Handi Kaoutar , Cara Orges , De Stradis Angelo , Valentini Franco , Elbeaino Toufic
Published: May 2024

Xylella fastidiosa (Xf) is a major phytosanitary threat to global agricultural production. The complexity and difficulty of controlling Xf underscore the pressing need for novel antibacterial agents, i.e., bacteriophages, which are natural predators of bacteria. In this study, a novel lytic bacteriophage of Xf subsp. pauca, namely Xylella phage MATE 2 (MATE 2), was isolated from sewage water in southern Italy. Biological characterization showed that MATE 2 possessed a broad-spectrum of antibacterial activity against various phytobacteria within the family Xanthomonadaceae, a rapid adsorption time (10 min), and high resistance to a broad range of pH (4–10) and temperatures (4–60°C). Most importantly, MATE 2 was able to suppress the growth of Xf subsp. pauca cells in liquid culture for 7 days, demonstrating its potential as an effective antibacterial agent against Xf. The genomic and electron microscopy analyses revealed that MATE 2 is a new species tentatively belonging to the genus Carpasinavirus within the class Caudoviricetes, with an isometric capsid head of 60 ± 5 nm along with a contractile tail of 120 ± 7.5 nm. Furthermore, the high-throughput sequencing and de novo assembly generated a single contig of 63,695 nucleotides in length; representing a complete genome composed of 95 Open Reading Frames. Bioinformatics analysis performed on MATE 2 genome revealed the absence of lysogenic mediated genes, and genes encoding virulence factors, antibiotic resistance, and toxins. This study adds a new phage to the very short list of Xf-infecting lytic phages, whose in-vitro antibacterial activity has been ascertained, while its efficacy on Xf-infected olive trees in the field has yet to be determined.
Exploratory analysis on the association of dietary live microbe and non-dietary prebiotic/probiotic intake with serum cotinine levels in the general adult population
Authors: Lin Shanhong , Zhu Ning , Zhu Yujing , Mao Haiping , Zhang Shengmin
Published: May 2024

Background

Previous research has indicated the potential involvement of the microbiota in smoking-related processes. The present study seeks to examine the relationship between dietary live microbes, as well as probiotic or prebiotic consumption, and serum cotinine levels.

Methods

This study used data from the National Health and Nutrition Examination Survey 1999–2018. Dietary intake information and probiotic/prebiotic intake data was collected through self-reported questionnaires. Participants were stratified into low, medium, and high intake groups according to their consumption of foods with varying microbial content. Multiple linear models were applied to explore the relationships of dietary live microbes, probiotic or prebiotic use with the serum cotinine level.

Results

A total of 42,000 eligible participants were included in the final analysis. The weighted median serum cotinine level was 0.05 (0.01, 10.90) ng/ml. Participants with low, medium, and high dietary microbe intake represented 35.4, 43.6, and 21.0% of the cohort, respectively. Furthermore, participants were stratified into three groups based on their overall consumption of foods with variable microbe contents. The association between dietary live microbe intake and serum cotinine levels remained robust across all models, with medium intake as the reference (Model 2: β = −0.14, 95% CI: −0.20, −0.07; High: β = −0.31, 95% CI: −0.39, −0.22). Moreover, both prebiotic and probiotic use exhibited an inverse relationship with serum cotinine levels (Prebiotic: β = −0.19, 95% CI: −0.37, −0.01; Probiotic: β = −0.47, 95% CI: −0.64, −0.30). Subgroup analyses revealed no discernible interactions between dietary live microbe, prebiotic, probiotic use, and serum cotinine levels.

Conclusion

Our findings suggest a negative correlation between dietary live microbe intake, as well as non-dietary prebiotic/probiotic consumption, and serum cotinine levels.
Performance of P16INK4a immunocytochemical stain in facilitating cytology interpretation of HSIL for HPV-positive women aged 50 and above
Authors: Hou Jun , Du Hui , Wang Chun , Song Fangbin , Qu Xinfeng , Wu Ruifang
Published: May 2024

Background

Few articles have focused on the cytological misinterpretation of high-grade squamous intraepithelial lesion (HSIL). Due to estrogen deficiency, cervical epithelial cells in postmenopausal women tend to show atrophic change that looks like HSIL on Papanicolaou-stained cytology slides, resulting in a higher rate of cytological misinterpretation. P16INK4a immunocytochemical staining (P16 cytology) can effectively differentiate diseased cells from normal atrophic ones with less dependence on cell morphology.

Objective

To evaluate the role of P16 cytology in differentiating cytology HSIL from benign atrophy in women aged 50 years and above.

Methods

Included in this analysis were women in a cervical cancer screening project conducted in central China who tested positive for high-risk human papillomavirus (hr-HPV) and returned back for triage with complete data of primary HPV testing, liquid-based cytology (LBC) analysis, P16 immuno-stained cytology interpretation, and pathology diagnosis. The included patients were grouped by age: ≥50 (1,127 cases) and <50 years (1,430 cases). The accuracy of LBC and P16 cytology in the detection of pathology ≥HSIL was compared between the two groups, and the role of P16 immuno-stain in differentiating benign cervical lesions from cytology ≥HSIL was further analyzed.

Results

One hundred sixty-seven women (14.8%; 167/1,127) in the ≥50 group and 255 (17.8%, 255/1,430) in the <50 group were pathologically diagnosed as HSIL (Path-HSIL). LBC [≥Atypical Squamous Cell Of Undetermined Significance (ASCUS)] and P16 cytology (positive) respectively detected 63.9% (163/255) and 90.2% (230/255) of the Path-≥HSIL cases in the <50 group and 74.3% (124/167) and 93.4% (124/167) of the Path-≥HSIL cases in the ≥50 group. LBC matched with pathology in 105 (41.2%) of the 255 Path-≥HSIL cases in the <50 group and 93 (55.7%) of the 167 Path-≥HSIL cases in the ≥50 group. There were five in the <50 group and 14 in the ≥50 group that were Path-≤LSIL cases, which were interpreted by LBC as HSIL, but negative in P16 cytology.

Conclusion

P16 cytology facilitates differentiation of Path-≤LSIL from LBC-≥HSIL for women 50 years of age and above. It can be used in the lower-resource areas, where qualified cytologists are insufficient, as the secondary screening test for women aged ≥50 to avoid unnecessary biopsies and misinterpretation of LBC primary or secondary screening.
Cancer incidence in immigrants by geographical area of origin: data from the Veneto Tumour Registry, Northeastern Italy
Authors: Ferroni Eliana , Guzzinati Stefano , Andreotti Alessandra , Baracco Susanna , Baracco Maddalena , Bovo Emanuela , Carpin Eva , Dal Cin Antonella , Greco Alessandra , Fiore Annarita , Memo Laura , Monetti Daniele , Rizzato Silvia , Stocco Jessica Elisabeth , Stocco Carmen , Zamberlan Sara , Zorzi Manuel
Published: May 2024

Objective

We investigated whether there are differences in cancer incidence by geographical area of origin in North-eastern Italy.

Methods

We selected all incident cases recorded in the Veneto Tumour Registry in the period 2015-2019. Subjects were classified, based on the country of birth, in six geographical areas of origin (Italy, Highly Developed Countries-HDC, Eastern Europe, Asia, Africa, South-central America). Age-standardized incidence rates and incidence rate ratio (IRR) were calculated, for all cancer sites and for colorectal, liver, breast and cervical cancer separately.

Results

We recorded 159,486 all-site cancer cases; 5.2% cases occurred in subjects born outside Italy, the majority from High Migratory Pressure Countries (HMPC) (74.3%). Incidence rates were significantly lower in subjects born in HMPC in both sexes. Immigrants, in particular born in Asia and Africa, showed lower rates of all site cancer incidence. The lowest IRR for colorectal cancer was observed in males from South-Central America (IRR 0.19, 95%CI 0.09-0.44) and in females from Asia (IRR 0.32, 95%CI 0.18-0.70). The IRR of breast cancer appeared significantly lower than Italian natives in all female populations, except for those coming from HDC. Females from Eastern Europe showed a higher IRR for cervical cancer (IRR 2.02, 95%CI 1.57-2.61).

Conclusion

Cancer incidence was found lower in subjects born outside Italy, with differences in incidence patterns depending on geographical area of origin and the cancer type in question. Further studies, focused on the country of birth of the immigrant population, would help to identify specific risk factors influencing cancer incidence.
The tumor-associated fibrotic reactions in microenvironment aggravate glioma chemoresistance
Authors: Xu Jiaqi , Zhang Ji , Chen Wubing , Ni Xiangrong
Published: May 2024

Malignant gliomas are one of the most common and lethal brain tumors with poor prognosis. Most patients with glioblastoma (GBM) die within 2 years of diagnosis, even after receiving standard treatments including surgery combined with concomitant radiotherapy and chemotherapy. Temozolomide (TMZ) is the first-line chemotherapeutic agent for gliomas, but the frequent acquisition of chemoresistance generally leads to its treatment failure. Thus, it’s urgent to investigate the strategies for overcoming glioma chemoresistance. Currently, many studies have elucidated that cancer chemoresistance is not only associated with the high expression of drug-resistance genes in glioma cells but also can be induced by the alterations of the tumor microenvironment (TME). Numerous studies have explored the use of antifibrosis drugs to sensitize chemotherapy in solid tumors, and surprisingly, these preclinical and clinical attempts have exhibited promising efficacy in treating certain types of cancer. However, it remains unclear how tumor-associated fibrotic alterations in the glioma microenvironment (GME) mediate chemoresistance. Furthermore, the possible mechanisms behind this phenomenon are yet to be determined. In this review, we have summarized the molecular mechanisms by which tumor-associated fibrotic reactions drive glioma transformation from a chemosensitive to a chemoresistant state. Additionally, we have outlined antitumor drugs with antifibrosis functions, suggesting that antifibrosis strategies may be effective in overcoming glioma chemoresistance through TME normalization.
Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant
Authors: Li Gengyun , Mo Yunrong , Lv Junheng , Han Shu , Fan Wei , Zhou Ying , Yang Zhengan , Deng Minghua , Xu Bin , Wang Yanyan , Zhao Kai
Published: May 2024

Verticillium wilt, caused by Verticillium dahliae, is a soil-borne disease affecting eggplant. Wild eggplant, recognized as an excellent disease-resistant resource against verticillium wilt, plays a pivotal role in grafting and breeding for disease resistance. However, the underlying resistance mechanisms of wild eggplant remain poorly understood. This study compared two wild eggplant varieties, LC-2 (high resistance) and LC-7 (sensitive) at the phenotypic, transcriptomic, and metabolomic levels to determine the molecular basis of their resistance to verticillium wilt. These two varieties exhibit substantial phenotypic differences in petal color, leaf spines, and fruit traits. Following inoculation with V. dahliae, LC-2 demonstrated significantly higher activities of polyphenol oxidase, superoxide dismutase, peroxidase, phenylalanine ammonia lyase, β-1,3 glucanase, and chitinase than did LC-7. RNA sequencing revealed 4,017 differentially expressed genes (DEGs), with a significant portion implicated in processes associated with disease resistance and growth. These processes encompassed defense responses, cell wall biogenesis, developmental processes, and biosynthesis of spermidine, cinnamic acid, and cutin. A gene co-expression analysis identified 13 transcription factors as hub genes in modules related to plant defense response. Some genes exhibited distinct expression patterns between LC-2 and LC-7, suggesting their crucial roles in responding to infection. Further, metabolome analysis identified 549 differentially accumulated metabolites (DAMs) between LC-2 and LC-7, primarily consisting of compounds such as flavonoids, phenolic acids, lipids, and other metabolites. Integrated transcriptome and metabolome analyses revealed the association of 35 gene–metabolite pairs in modules related to the plant defense response, highlighting the interconnected processes underlying the plant defense response. These findings characterize the molecular basis of LC-2 resistance to verticillium wilt and thus have potential value for future breeding of wilt-resistant eggplant varieties.
Poor sleep quality may trigger cognitive deficits after recovery from COVID-19
Authors: Carnes-Vendrell A. , Piñol-Ripoll G. , Ariza M. , Cano N. , Segura B. , Junque C. , Béjar J. , Barrue C. , Garolera M. ,   Nautilus Project Collaborative Group
Published: May 2024

Objective

In the present study, we aimed to assess the cognition of post-COVID-19 condition (PCC) participants in relation to their subjective sleep quality (Pittsburgh Sleep Quality Index, PSQI) and to analyse possible moderators of this effect, such as quality of life (European Quality of Life-5 Dimensions, EQ-5D), fatigue (Chadler Fatigue Questionnaire, CFQ), cognitive reserve (Cognitive Reserve Questionnaire, CRC), and subjective cognitive complaints (Memory Failures of Everyday Questionnaire, MFE-30).

Methods

We included 373 individuals with PCC and 126 healthy controls (HCs) from the NAUTILUS Project (NCT05307549 and NCT05307575) who were assessed with a comprehensive neuropsychological battery and various questionnaires.

Results

We found that PCC participants with poor sleep quality had a 4.3% greater risk of immediate verbal memory deficits than those with good sleep quality, as indicated by the greater odds ratio (OR) of 1.043 and confidence interval (CI) of 1.023–1.063. Additionally, their risk of immediate verbal memory disorders was multiplied by 2.4 when their EQ-5D score was low (OR 0.33; CI 0.145–0.748), and they had a lower risk of delayed visual memory deficits with a greater CRC (OR 0.963; CI 0.929–0.999). With respect to processing speed, PCC participants with poor sleep quality had a 6.7% greater risk of deficits as the MFE increased (OR 1.059; CI 1.024–1.096), and the risk of slowed processing speed tripled with a lower EQ-5D (OR 0.021; CI 0.003–0.141).

Conclusion

These results indicate that poor subjective sleep quality is a potential trigger for cognitive deficits. Therapeutic strategies to maximize sleep quality could include reducing sleep disturbances and perhaps cognitive impairment in PCC individuals.
Successful remote treatment of a client with Hikikomori using internet-delivered cognitive therapy for social anxiety disorder: a case report
Authors: Sakai Motohiro , Yoshinaga Naoki , Thew Graham R. , Clark David M.
Published: May 2024

Hikikomori (prolonged social withdrawal) has been discussed as a hidden worldwide epidemic and a significant social and healthcare issue. Social anxiety disorder is the most common psychiatric disorder preceding the onset of Hikikomori. Although studies exist suggesting the effectiveness of family-support interventions, little is known about psychotherapeutic approaches for Hikikomori individuals. Here, we present a case of Hikikomori wherein an internet-delivered cognitive therapy for social anxiety disorder (iCT-SAD) worked effectively in improving the client’s social anxiety symptoms and social interaction behaviors. This case study demonstrates the principle that evidence-based psychological interventions focusing on social anxiety can be effective for clients with Hikikomori. Furthermore, the online mode of treatment delivery, along with a variety of relevant modules, may facilitate clients’ engagement with treatment at home. The findings suggest that iCT-SAD might be a promising option for Hikikomori clients who have social anxiety problems, within the recommended stepped-intervention approach.
Identifying determinants of spatial agglomeration of healthcare resources by using spatial econometric methods: a longitudinal study in China
Authors: Dong Enhong , Sun Xiaoting , Xi Yueming , Wang Yijia , Wang Tao , Gao Weimin
Published: May 2024

Background

Healthcare resources are necessary for individuals to maintain their health. The Chinese government has implemented policies to optimize the allocation of healthcare resources and achieve the goal of equality in healthcare for the Chinese people since the implementation of the new medical reform in 2009. Given that no study has investigated regional differences from the perspective of healthcare resource agglomeration, this study aimed to investigate China’s healthcare agglomeration from 2009 to 2017 in China and identify its determinants to provide theoretical evidence for the government to develop and implement scientific and rational healthcare policies.

Methods

The study was conducted using 2009–2017 data to analyze health-resource agglomeration on institutions, beds, and workforce in China. An agglomeration index was applied to evaluate the degree of regional differences in healthcare resource allocation, and spatial econometric models were constructed to identify determinants of the spatial agglomeration of healthcare resources.

Results

From 2009 to 2017, all the agglomeration indexes of healthcare exhibited a downward trend except for the number of institutions in China. Population density (PD), government health expenditures (GHE), urban resident’s disposable income (URDI), geographical location (GL), and urbanization level (UL) all had positive significant effects on the agglomeration of beds, whereas both per capita health expenditures (PCHE), number of college students (NCS), and maternal mortality rate (MMR) had significant negative effects on the agglomeration of institutions, beds, and the workforce. In addition, population density (PD) and per capita gross domestic product (PCGDP) in one province had negative spatial spillover effects on the agglomeration of beds and the workforce in neighboring provinces. However, MMR had a positive spatial spillover effect on the agglomeration of beds and the workforce in those regions.

Conclusion

The agglomeration of healthcare resources was observed to remain at an ideal level in China from 2009 to 2017. According to the significant determinants, some corresponding targeted measures for the Chinese government and other developing countries should be fully developed to balance regional disparities in the agglomeration of healthcare resources across administrative regions.
The spatial spillover effect of green technology innovation on water pollution–evidence from 283 Chinese cities
Authors: Ruan Yuling , Zhang Anqi
Published: May 2024

While economic development brings serious environmental problems, technological advances can effectively reduce pollution, which helps to achieve the Sustainable Development Goals. Although the impact of green technology innovation on atmospheric pollutants and carbon emissions has been extensively studied, the effect of such innovation on pollutant reduction varies due to the diverse regional distribution characteristics of different pollutants. Thus, this paper contributes to the literature by examining the influence of green technology innovation on water pollution from a regional perspective, with a particular emphasis on the pronounced clustering of wastewater pollution in China's coastal areas. Both Fixed-Effect model and spatial analysis are adopted. Our findings reveal a significant U-shaped relationship between technology innovation and water pollution, as measured by both industrial wastewater and the ratio of unprocessed sewage. Interestingly, this pollution reduction effect also exhibits a U-shaped spatial spillover. Given the rapid development of the digital economy, it can further amplify the spatial spillover effect of green innovation, especially in eastern regions. This study also provides recent empirical evidence from China to the Environmental Kuznets Curve.
Impact of contract farming on green technological efficiency of farmers: a comparative study of two contract organizational models
Authors: Li Qi , Wang Zixuan
Published: May 2024

Introduction

Engaging in contract farming represents a crucial avenue for developing countries to integrate small farmers into modern agricultural practices. Existing research believes that contract farming offers a promising opportunity for fostering sustainable agricultural development. However, insufficient attention has been directed toward investigating the varying impacts of different organizational models within contract farming on farmers' transition to green production practices.

Methods

This study investigates the impact of contract farming on green technological efficiency of farmers using survey data from 719 wheat growers in Shandong Province, China. The propensity score matching method and multiple mediation effects models are employed for empirical analysis.

Results

The organizational model plays a pivotal role in determining whether participation in contract farming can improve the green technological efficiency of farmers. Participation in an integrated model demonstrates a significant effect on improving green technological efficiency, whereas participation in a quasi-integrated model does not show a significant improvement. Integrated contract farming can improve green technological efficiency through expanding the degree of land consolidation and enhancing the level of productive service, whereas quasi-integrated contract farming improves green technological efficiency only by enhancing production intensification. As an economic incentive measure, the effect of contract purchase price on strengthening the enhancement of green technological efficiency in the quasi-integrated model is moderate only when the contract purchase price exceeds the local average selling price of wheat by more than 19.3%. Conversely, the moderate effect of farmers' dividend income in the integrated model remains consistent, with higher dividend incomes correlating with a stronger moderating effect.

Discussion

The impact of contract farming on green technology efficiency correlates significantly with the organizational model. Compared with the quasi-integrated contract farming model, the integrated model distinctly excels in advancing farmers' green technology efficiency, evidenced by its ability to consolidate fragmented land, provide productive services, and offer economic incentives. Therefore, to unlock the latent potential of contract farming in driving agricultural green transformation, a shift toward organizational models with higher levels of integration is essential.
The DaNa projects: public communication of (nano)material safety data—from conspiracy theories to study quality
Authors: Kühnel Dana , Krug Harald F. , Steinbach Christoph , Nau Katja
Published: May 2024

In this perspective, the authors give their view on the developments and experiences on communicating on (nano)materials safety. We would like to share our experiences with the scientific community in order to make them useful for future communication activities. We present the long-term work of the science communication projects DaNa, DaNa2.0 and DaNa4.0, running from 2009 to 2023. Starting in the early 2000s with the beginnings of nanotechnology research, communication on the safety of nanomaterials with the public was still very new and faced the projects with many challenges. Today, science communication is indispensable for the dissemination of scientific findings and a fact-based approach like the DaNa “Knowledge Base Materials” creates a trustworthy dialogue with the public. This long-term project series has made a significant contribution to communication on the safety of nanomaterials, perhaps even the largest among publicly funded project series worldwide.
Adolescents’ Perspectives on Coping with Bullying in the Digital Environment
Authors: Paryente Bilha
Published: May 2024

This study examined the coping process of adolescents in the construction of a sense of coherence after experiencing bullying events in the digital environment. The content analysis of semi-structured interviews with 20 adolescents aged 14 to 18 (M = 16.85) revealed three themes: (1) the comprehension of the bullying as stemming from hatred and boredom; (2) managing the bullying by turning to the bully asking that they stop, deleting and forgiving after the event, and coping through self-isolation and with the support of family and friends; and (3) the emotional processing of anger, embarrassment, and sadness. All the data correlated with the a priori themes of the salutogenic approach, demonstrating a significant expression of the components of a sense of coherence. Understanding the adolescents’ coping process and their construction of a sense of coherence following their experience of cyberbullying may help practitioners design and implement tailored interventions for adolescents and their educators who are increasingly confronted with this issue.
Multiple-Bird-Strike Probability Model and Dynamic Response of Engine Fan Blades
Authors: Wang Siqi , Li Jinhui , Lin Haidong , Deng Zhenhong , Zhang Baoqiang , Luo Huageng
Published: May 2024

Bird strikes pose one of the most significant threats to aviation safety, often leading to substantial loss of life and economic damage. Many bird strike incidents involve multiple birds. However, in previous bird strike studies, the problem of multiple bird strikes has often been neglected. In this paper, the bird slicing process of a rotating engine fan is examined, and a probability model is introduced to assess the risk of multiple impacts on the fan blades. In addition, this paper utilized an implicit–explicit calculation method. The parameters of blade root stress, tip displacement, plastic deformation, and energy were selected to investigate the effects of the time interval and strike position of a bird strike on the dynamic response of and damage to the blades. The results indicated that the position of bird strikes has a more pronounced effect on blade damage compared to the time interval between impacts. Damage to a blade is most severe when the blade root is struck multiple times. Multiple bird strikes may not always lead to a significant increase in maximum blade tip displacement, and may even have a dampening effect.
Evolution of Industrial Quality Parameters of Wheat during Storage in White and Colored Silo Bags: A Field-Scale Study
Authors: Bartosik Ricardo Enrique , Cardoso Marcelo Leandro , Carpaneto Barbara Bettina , Astiz Valentina , Molfese Elena Rosa , de la Torre Diego Antonio
Published: May 2024

Over the past two decades, the silo bag system has gained popularity for storing grains and by-products under hermetic conditions. However, the impact of higher temperatures in the outer grain layer on key industrial parameters, such as wheat baking quality, remains insufficiently understood. Traditional silo bags are black on the inside and white on the outside to reflect sunlight, but colored bags, recently introduced to the market, absorb more heat, potentially warming the grain and causing damage. This study aimed to assess the effect of grain strata and bag color on grain temperature and quality under field conditions. Results showed a significant surface temperature increase in colored bags compared to white ones, approximately 3 °C, which affected the temperature of the peripheral grain layer. Moisture content slightly increased (0.2 percentage points) in the outer grain layer. However, many industrial quality parameters (protein content, P/L, W, and loaf volume) and the germination test for wheat, showed no significant differences between colored and white bags or between different strata after 120 days of storage, although the falling number increased and wet gluten decreased. These findings suggest that, despite surface temperature differences, the overall industrial quality of wheat remains unaffected by external bag coloration. The influence of ambient temperature on the peripheral layer was estimated to affect approximately 5–10% of the grain mass, indicating that adverse impacts on grain quality may go unnoticed without implementing stratified sampling techniques.
Isolation, Identification and Characterization of Leptosphaerulina trifolii, the Causative Agent of Alfalfa Leptosphaerulina Leaf Spot in Inner Mongolia, China
Authors: Huo Hongli , Huangfu Jiuru , Song Peiling , Zhang Dongmei , Shi Zhidan , Zhao Lili , Li Ziqin , Zhou Hongyou
Published: May 2024

Leptosphaerulina leaf spot, caused by Leptosphaerulina trifolii, is a major disease of alfalfa (Medicago sativa), leading to noticeable losses. From 2022 to 2023, we collected samples of alfalfa with symptoms of the disease from different locations in Inner Mongolia, China. Nine fungal isolates recovered from these samples were identified through morphological traits and a maximum likelihood phylogeny based on concatenated partial sequences of ITS, 28S, and rpb2. A pathogenicity test on alfalfa confirmed the pathogenicity of the isolates on alfalfa. Analysis of physiological traits of L. trifolii revealed optimal mycelium growth at 20 °C and a pH range of 5 to 7, with soluble starch as the preferred carbon source and yeast extract as the optimal nitrogen source. The pathogen thrived in V8-juice agar and oat agar media. This study confirms L. trifolii as the causative agent of Leptosphaerulina leaf spot of alfalfa in Inner Mongolia and provides valuable insights into its optimal growth conditions. These findings enhance the understanding and management of this disease in alfalfa fields.
Implications of Lairage and Environmental Enrichment on Behavioral Responses and Skin Lesions in Finishing Pigs in a Slaughterhouse
Authors: Sobral Vivian Schwaab , Silveira Robson Mateus Freitas , Guesine Giovane Debs , Arno Alessandra , de Azevedo Karen Airosa Machado , Lobos Cristian Marcelo Villegas , da Silva Iran José Oliveira
Published: May 2024

Different resources, such as environmental enrichment, are being evaluated in order to minimize animal stress and promote better conditions during the life cycle of animals, as consumers are increasingly concerned about animal welfare issues. Lairage represents an important stage in the swine production chain and is directly related to animal welfare. The aim of this study was to evaluate the effect of lairage time in the slaughterhouse and environmental enrichment on the level of skin lesions and behavioral responses in pigs. A total of 648 finishing pigs of both sexes were assessed before and after lairage at the slaughterhouse with a five-point scale (0 = none, to 4 = ≥16 superficial lesions or >10 deep lesions). After lairage (after slaughter), lesions were also classified according to their source (mounting, fighting, and handling). Pigs were distributed into two treatments groups during lairage: with environmental enrichment (EE) on the pen, with hanging metal chains, and with no enrichment (NE). Behavior was monitored during the first four hours of lairage. Proportional odds, mixed linear model for longitudinal data, and non-parametric Wilcoxon signed rank tests were used to analyze the relation between treatments, skin lesions, and behavior. The simple metal chains did not affect skin lesion score or pigs’ behavior (p > 0.05), whereas lairage duration influenced standing (SA), sitting (S), lying (L), idleness (I), and drinking water (D) (p < 0.001). The main source of skin lesions was handling, which did not differ between treatments (EE and NE) (p > 0.05). It was observed that the severity of the lesions (highest scores of 3, 4, and 5) increased in the different anatomical regions of the pigs when compared before and after slaughter, with the exception of the frontal area, which was the same (p = 0.7547). Lairage time has a proportional relation with skin lesions, and hanging chains at the slaughterhouse pens was not enough to reduce the number of lesions and to change pig behavior.
The Role of Curcumin in Oral Health and Diseases: A Systematic Review
Authors: Inchingolo Francesco , Inchingolo Alessio Danilo , Latini Giulia , Trilli Irma , Ferrante Laura , Nardelli Paola , Malcangi Giuseppina , Inchingolo Angelo Michele , Mancini Antonio , Palermo Andrea , Dipalma Gianna
Published: May 2024

Curcumin (Curcumin) belongs to the polyphenol family. It is extracted by drying the root of a plant of Asian origin, belonging to the Zingiberaceae family. The best-known species is Curcumincuma Longa. Curcumin has been recognized as having great therapeutic powers since ancient times. Studies on curcumin have since confirmed its powerful antioxidant properties, preventing both the formation of free radicals and their neutralization, having anti-inflammatory, antibacterial, immunological, and neuroprotective properties, as well as being a regulator of the intestinal microbiota with beneficial effects on the clinical manifestations of metabolic syndrome. Our study aimed to highlight how all these therapeutic aspects could benefit oral health, both preventing and improving the course of pathological processes. The effect of mouthwashes, and curcumin-based gels on the regulation of bacterial plaque and in the control of gingivitis, was largely comparable to that of using 0.20% chlorhexidine, with fewer side effects. Being a highly hydrophobic substance, it has a high permeability to cross the cell membrane. Bioavailability increases when combined with liposoluble substances (e.g., olive oil) and piperine, which improves absorption. Curcumin also has a negligible degree of toxicity, making it an excellent alternative to the use of gold standard products for oral disinfection.