bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors associated with the need for inotropic support following pulmonary artery banding surgery for CHD
Authors: Mastropietro Christopher W. , Clark Andrea B. , Loke Katie L. , Chaudhry Paulomi , Cossu Anne E. , Patel Jyoti K. , Herrmann Jeremy L.
Published: March 2023

Objective:

We aimed to identify factors independently associated with the need for inotropic support for low cardiac output or haemodynamic instability after pulmonary artery banding surgery for CHD.

Methods:

We performed a retrospective chart review of all neonates and infants who underwent pulmonary banding between January 2016 and June 2019 at our institution. Bivariate and multivariable analyses were performed to identify factors independently associated with the use of post-operative inotropic support, defined as the initiation of inotropic infusion(s) for depressed myocardial function, hypotension, or compromised perfusion within 24 hours of pulmonary artery banding.

Results:

We reviewed 61 patients. Median age at surgery was 10 days (25%,75%:7,30). Cardiac anatomy was biventricular in 38 patients (62%), hypoplastic right ventricle in 14 patients (23%), and hypoplastic left ventricle in 9 patients (15%). Inotropic support was implemented in 30 patients (49%). Baseline characteristics of patients who received inotropic support, including ventricular anatomy and pre-operative ventricular function, were not statistically different from the rest of the cohort. Patients who received inotropic support, however, were exposed to larger cumulative doses of ketamine intraoperatively – median 4.0 mg/kg (25%,75%:2.8,5.9) versus 1.8 mg/kg (25%,75%:0.9,4.5), p < 0.001. In a multivariable model, cumulative ketamine dose greater than 2.5mg/kg was associated with post-operative inotropic support (odds ratio 5.5; 95% confidence interval: 1.7,17.8), independent of total surgery time.

Conclusions:

Inotropic support was administered in approximately half of patients who underwent pulmonary artery banding and more commonly occurred in patients who received higher cumulative doses of ketamine intraoperatively, independent of the duration of surgery.
Plication for diaphragm paralysis after paediatric cardiac surgery: a single-centre experience
Authors: Simsek Baran , Ozyuksel Arda , Saygi Murat , Bilal Mehmet Salih
Published: March 2023

Objective:

Diaphragm paralysis is a well-known complication following surgery for CHDs, which increases morbidity, mortality, and length of hospital stay as well as costs. Herein, we present our experience with diaphragm plication following paralysis of the phrenic nerve encountered after paediatric cardiac surgery.

Methods:

This study retrospectively reviewed the medical records of 23 diaphragm plications in 20 patients who underwent paediatric cardiac surgery between January 2012 and January 2022. The patients were carefully selected based on aetiology and a combination of clinical manifestation and chest imaging characteristics including chest X-ray, ultrasonography, and fluoroscopy.

Results:

Twenty-three successful plications were performed in 20 patients (15 males and 5 females) out of a total of 1938 operations performed in our centre. Mean age and body weight were 18.2 ± 17.1 months and 8.3 ± 3.7 kg, respectively. The period between the cardiac surgery and diaphragmatic plication was 18.7 ± 15.1 days. The highest incidence of diaphragm paralysis was encountered in systemic to pulmonary artery shunt patients with 7 out of 152 patients (4.6%). Any mortality was not encountered during a mean follow-up period of 4.3 ± 2.6 years.

Conclusions:

Early results of plication of the diaphragm following phrenic nerve palsy in symptomatic patients who underwent paediatric cardiac surgery are encouraging. Evaluation of the diaphragmatic function should be a routine part of post-operative echocardiography. Diaphragm paralysis may be a consequence of dissection, contusion, stretching, and thermal injury both in terms of hypothermia and hyperthermia.
Immediate Medical Care Rendered by US Law Enforcement Officers after Officer-Involved Shootings – An Open-Access Public Domain Video Analysis
Authors: McGuire Sarayna S. , Keim Audrey , Blakeney Craig A. , Brand Shari I. , Klassen Aaron B. , Luke Anuradha , Maher Steven A. , Wood Jeffrey M. , Sztajnkrycer Matthew D.
Published: March 2023

Background:

After officer-involved shootings (OIS), rapid delivery of emergency medical care is critical but may be delayed due to scene safety concerns. The purpose of this study was to describe medical care rendered by law enforcement officers (LEOs) after lethal force incidents.

Methods:

Retrospective analysis of open-source video footage of OIS occurring from February 15, 2013 through December 31, 2020. Frequency and nature of care provided, time until LEO and Emergency Medical Services (EMS) care, and mortality outcomes were evaluated. The study was deemed exempt by the Mayo Clinic Institutional Review Board.

Results:

Three hundred forty-two (342) videos were included in the final analysis; LEOs rendered care in 172 (50.3%) incidents. Average elapsed time from time-of-injury (TOI) to LEO-provided care was 155.8 (SD = 198.8) seconds. Hemorrhage control was the most common intervention performed. An average of 214.2 seconds elapsed between LEO care and EMS arrival. No mortality difference was identified between LEO versus EMS care (P = .1631). Subjects with truncal wounds were more likely to die than those with extremity wounds (P < .00001).

Conclusions:

It was found that LEOs rendered medical care in one-half of all OIS incidents, initiating care on average 3.5 minutes prior to EMS arrival. Although no significant mortality difference was noted for LEO versus EMS care, this finding must be interpreted cautiously, as specific interventions, such as extremity hemorrhage control, may have impacted select patients. Future studies are needed to determine optimal LEO care for these patients.
Genetic diversity of quinoa (Chenopodium quinoa Willd.) from Cundinamarca, Colombia
Authors: Morillo-Coronado Ana Cruz , Manjarres-Hernández Elsa Helena , Pedreros-Benavides María Camila
Published: March 2023

Chenopodium quinoa W. is a species of South America with an exceptional nutritional content and wide agroclimatological adaptation. It has great genetic and phenotypic variability, however in Colombia there are few genetic improvement programmes that take advantage of its great genetic and productive potential. In Cundinamarca there are some adapted genotypes which have been selected by farmers. We evaluated 36 genotypes of Blanca de Jericó, Blanca Subachoque, Aurora, Púrpura and Tunkahuan from Cundinamarca, using eight ISSR markers. The analysis by the coefficient of Nei-Li at the level of similarity of 0.40 divided the population into three groups according their background genetic and the colour of oxalates. The percentage of polymorphic loci was higher than 90%. The average value of heterozygosity was 0.32, which is low given the selection processes that the evaluated germplasm has undergone. We found moderate genetic differentiation (Fst = 0.23). The analysis of molecular variance (AMOVA) showed higher variation (77%) groups than among groups (23%). The results revealed intra-population diversity, which suggests that farmers within their farms should undergo a more rigorous seed selection process. Our results demonstrate that ISSR markers are useful and powerful to assess the genetic relationships, polymorphism and genetic diversity of quinoa cultivars. The genetic characterization results reported in the present study will be promising for guiding the breeding of quinoa seed quality in Colombia.
Development of a set of InDel markers for characterizing three japonica rice (Oryza sativa L.) varieties
Authors: Wang Hongmei , Zhu Yue , Xiao Chujian , Yuan Jianming , Liu Yaoguang , Zhang Qunyu
Published: March 2023

The use of molecular markers is one of the most sensitive, powerful technologies for genetic purity assessment of seed lots. In this study, we aimed to develop a set of insertion and deletion (InDel) markers, through bioinformatics approaches, that may effectively distinguish three representative japonica rice (Oryza sativa L.) varieties, Nipponbare, Taichung 65 and Zhonghua11. The published whole-genome sequences of these varieties were aligned using BWA-MEM, followed by manual inspection for InDels of more than ten base pairs in size with the tview function of SAMtools. A set of ten InDel markers were thus identified and then validated by PCR in the three japonica rice varieties and their intercross F1 hybrids. Results showed that the InDel markers developed in this study could reliably distinguish these three japonica rice varieties. These molecular markers together with the detection method developed here can be applied to DNA-based genetic purity evaluation in rice breeding.
Interweaving influences and adaptations: sartorial endeavors of Okakura Kakuzō and M. K. Gandhi
Authors: Banerjee Maumita
Published: March 2023

Universalism, as a historical category, played an important ideological role in forging political solidarities beyond national boundaries in the modern period. The paper traces this idea in modern Asia through the sartorial styles of two intellectuals, Okakura Kakuzō and Mohandas Karamchand Gandhi. Clothing as a medium of inquiry offers a unique scholarly perspective to articulate the role of universalism vis-à-vis nationalism in colonial India and modern Japan. Since dress politics existed in Eastern and Western societies, it allows us to study lived experiences through a transregional dialog. Both men recognized clothing as an effective political lexicon to fashion the self and creatively include others within the ideological space. Due to their early exposure to various cultures, the clothing style adopted by Okakura and Gandhi was founded on notions of plurality and belonging to multiple places and people. Their positionality enabled them to establish a dialog with both national and imperial politics and dress in a style that was self-made and world-aware. The paper uses their photographs and writings from a period that engendered the practice of universalism and challenged the narratives of nationalism.
Effect of osmotic potential and temperature on germination of kochia (Bassia scoparia) populations from the U.S. Great Plains
Authors: Yadav Ramawatar , Jha Prashant , Kniss Andrew R. , Lawrence Nevin C. , Sbatella Gustavo M.
Published: March 2023

Development of integrated weed management strategies requires knowledge of weed emergence timing and patterns, which are regulated primarily by water and thermal requirements for seed germination. Laboratory experiments were conducted in fall 2017 to fall 2018 to quantify the effect of osmotic potential and temperature on germination of 44 kochia [Bassia scoparia (L.) A.J. Scott] populations under controlled conditions. Bassia scoparia populations were collected in fall 2016 from northern (near Huntley, MT, and Powell, WY) and southern (near Lingle, WY, and Scottsbluff, NE) regions of the U.S. Great Plains. Ten osmotic potentials from 0 to −2.1 MPa and eight constant temperatures from 4 to 26 C were evaluated. Response of B. scoparia populations to osmotic potential did not differ between the northern and southern regions. At an osmotic potential of 0 MPa, all B. scoparia populations had greater than 98% germination, and the time to achieve 50% germination (t50) was less than 1 d. At −1.6 MPa, 25% of seeds of all B. scoparia populations germinated. Osmotic potentials of −0.85 and −1.9 MPa reduced B. scoparia germination by 10% and 90%, respectively. Regardless of temperature regime, all populations exhibited greater than 88% germination. The germination rate was highest at temperatures between 15 to 26 C and did not differ between populations from northern versus southern regions. At this temperature range, all populations had a t50 of less than 1 d. However, at 4 C, B. scoparia populations from the northern region had a higher germination rate (5 h) and cumulative germination (7%) than populations from the southern region. Overall, these results indicate a wide range of optimum temperatures and osmotic potential requirements for B. scoparia germination.
Depth‐Dependent Slow Earthquakes Controlled by Temperature Dependence of Brittle‐Ductile Transitional Rheology
Authors: Ando Ryosuke , Ujiie Kohtaro , Nishiyama Naoki , Mori Yasushi
Published: March 2023

The discovery of slow earthquakes illuminates the existence of a strange depth dependence of seismogenesis, which contradicts the common understanding of smooth brittle/seismic‐ductile/aseismic transition as going deeper into the earth's surface layers. However, within the transitional layer on plate interfaces, observations have clarified slip velocities of slow earthquakes changing from those slower to faster with increasing depth, as described by the “seismogenic inversion layer.” We propose a new mechanical model that can consistently explain the classic brittle‐ductile transition and this inversion phenomenon by considering the heterogeneous fault zone composed of brittle blocks in the ductile matrix. The key mechanism is the interplay between the volumetric fraction of brittle blocks and the viscosity of the surrounding plastically deformed matrix, where the former and the latter decrease with increasing temperature. This model is extended to shallow‐slow earthquakes. Our results open a new pathway to infer the deformation mechanisms underlying slow earthquakes.
Increased retinoic acid signaling decreases lung metastasis in salivary adenoid cystic carcinoma by inhibiting the noncanonical Notch1 pathway
Authors: Zhou Meng-jiao , Yang Jia-jie , Ma Ting-yao , Feng Ge-xuan , Wang Xue-lian , Wang Li-Yong , Ge Yu-ze , Gao Ran , Liu Hong-liang , Shan Lin , Kong Lu , Chen Xiao-hong
Published: March 2023

MYB-NFIB fusion and NOTCH1 mutation are common hallmark genetic events in salivary gland adenoid cystic carcinoma (SACC). However, abnormal expression of MYB and NOTCH1 is also observed in patients without MYB-NFIB fusion and NOTCH1 mutation. Here, we explore in-depth the molecular mechanisms of lung metastasis through single-cell RNA sequencing (scRNA-seq) and exome target capture sequencing in two SACC patients without MYB-NFIB fusion and NOTCH1 mutation. Twenty-five types of cells in primary and metastatic tissues were identified via Seurat clustering and categorized into four main stages ranging from near-normal to cancer-based on the abundance of each cell cluster in normal tissue. In this context, we identified the Notch signaling pathway enrichment in almost all cancer cells; RNA velocity, trajectory, and sub-clustering analyses were performed to deeply investigate cancer progenitor-like cell clusters in primary tumor-associated lung metastases, and signature genes of progenitor-like cells were enriched in the “MYC_TARGETS_V2” gene set. In vitro, we detected the NICD1-MYB-MYC complex by co-immunoprecipitation (Co-IP) and incidentally identified retinoic acid (RA) as an endogenous antagonist of genes in the “MYC_TARGETS_V2” gene set. Following this, we confirmed that all-trans retinoic acid (ATRA) suppresses the lung metastasis of SACC by correcting erroneous cell differentiation mainly caused by aberrant NOTCH1 or MYB expression. Bioinformatic, RNA-seq, and immunohistochemical (IHC) analyses of primary tissues and metastatic lung tissues from patients with SACC suggested that RA system insufficiency partially promotes lung metastasis. These findings imply the value of the RA system in diagnosis and treatment.
Human brain organoid model of maternal immune activation identifies radial glia cells as selectively vulnerable
Authors: Sarieva Kseniia , Kagermeier Theresa , Khakipoor Shokoufeh , Atay Ezgi , Yentür Zeynep , Becker Katharina , Mayer Simone
Published: March 2023

Maternal immune activation (MIA) during critical windows of gestation is correlated with long-term neurodevelopmental deficits in the offspring, including increased risk for autism spectrum disorder (ASD) in humans. Interleukin 6 (IL-6) derived from the gestational parent is one of the major molecular mediators by which MIA alters the developing brain. In this study, we establish a human three-dimensional (3D) in vitro model of MIA by treating induced pluripotent stem cell-derived dorsal forebrain organoids with a constitutively active form of IL-6, Hyper-IL-6. We validate our model by showing that dorsal forebrain organoids express the molecular machinery necessary for responding to Hyper-IL-6 and activate STAT signaling upon Hyper-IL-6 treatment. RNA sequencing analysis reveals the upregulation of major histocompatibility complex class I (MHCI) genes in response to Hyper-IL-6 exposure, which have been implicated with ASD. We find a small increase in the proportion of radial glia cells after Hyper-IL-6 treatment through immunohistochemistry and single-cell RNA-sequencing. We further show that radial glia cells are the cell type with the highest number of differentially expressed genes, and Hyper-IL-6 treatment leads to the downregulation of genes related to protein translation in line with a mouse model of MIA. Additionally, we identify differentially expressed genes not found in mouse models of MIA, which might drive species-specific responses to MIA. Finally, we show abnormal cortical layering as a long-term consequence of Hyper-IL-6 treatment. In summary, we establish a human 3D model of MIA, which can be used to study the cellular and molecular mechanisms underlying the increased risk for developing disorders such as ASD.
Phosphorylation of PHF2 by AMPK releases the repressive H3K9me2 and inhibits cancer metastasis
Authors: Dong Ying , Hu Hao , Zhang Xuan , Zhang Yunkai , Sun Xin , Wang Hanlin , Kan Weijuan , Tan Min-jia , Shi Hong , Zang Yi , Li Jia
Published: March 2023

Epithelial to mesenchymal transition (EMT) plays a crucial role in cancer metastasis, accompanied with vast epigenetic changes. AMP-activated protein kinase (AMPK), a cellular energy sensor, plays regulatory roles in multiple biological processes. Although a few studies have shed light on AMPK regulating cancer metastasis, the inside epigenetic mechanisms remain unknown. Herein we show that AMPK activation by metformin relieves the repressive H3K9me2-mediated silencing of epithelial genes (e.g., CDH1) during EMT processes and inhibits lung cancer metastasis. PHF2, a H3K9me2 demethylase, was identified to interact with AMPKα2. Genetic deletion of PHF2 aggravates lung cancer metastasis and abolishes the H3K9me2 downregulation and anti-metastasis effect of metformin. Mechanistically, AMPK phosphorylates PHF2 at S655 site, enhancing PHF2 demethylation activity and triggering the transcription of CDH1. Furthermore, the PHF2-S655E mutant that mimics AMPK-mediated phosphorylation status further reduces H3K9me2 and suppresses lung cancer metastasis, while PHF2-S655A mutant presents opposite phenotype and reverses the anti-metastasis effect of metformin. PHF2-S655 phosphorylation strikingly reduces in lung cancer patients and the higher phosphorylation level predicts better survival. Altogether, we reveal the mechanism of AMPK inhibiting lung cancer metastasis via PHF2 mediated H3K9me2 demethylation, thereby promoting the clinical application of metformin and highlighting PHF2 as the potential epigenetic target in cancer metastasis.
CD36+ cancer-associated fibroblasts provide immunosuppressive microenvironment for hepatocellular carcinoma via secretion of macrophage migration inhibitory factor
Authors: Zhu Gui-Qi , Tang Zheng , Huang Run , Qu Wei-Feng , Fang Yuan , Yang Rui , Tao Chen-Yang , Gao Jun , Wu Xiao-Ling , Sun Hai-Xiang , Zhou Yu-Fu , Song Shu-Shu , Ding Zhen-Bin , Dai Zhi , Zhou Jian , Ye Dan , Wu Duo-Jiao , Liu Wei-Ren , Fan Jia , Shi Ying-Hong
Published: March 2023

Hepatocellular carcinoma (HCC) is an immunotherapy-resistant malignancy characterized by high cellular heterogeneity. The diversity of cell types and the interplay between tumor and non-tumor cells remain to be clarified. Single cell RNA sequencing of human and mouse HCC tumors revealed heterogeneity of cancer-associated fibroblast (CAF). Cross-species analysis determined the prominent CD36+ CAFs exhibited high-level lipid metabolism and expression of macrophage migration inhibitory factor (MIF). Lineage-tracing assays showed CD36+CAFs were derived from hepatic stellate cells. Furthermore, CD36 mediated oxidized LDL uptake-dependent MIF expression via lipid peroxidation/p38/CEBPs axis in CD36+ CAFs, which recruited CD33+myeloid-derived suppressor cells (MDSCs) in MIF- and CD74-dependent manner. Co-implantation of CD36+ CAFs with HCC cells promotes HCC progression in vivo. Finally, CD36 inhibitor synergizes with anti-PD-1 immunotherapy by restoring antitumor T-cell responses in HCC. Our work underscores the importance of elucidating the function of specific CAF subset in understanding the interplay between the tumor microenvironment and immune system.
Manipulating the ordered oxygen complexes to achieve high strength and ductility in medium-entropy alloys
Authors: Jiao Meiyuan , Lei Zhifeng , Wu Yuan , Du Jinlong , Zhou Xiao-Ye , Li Wenyue , Yuan Xiaoyuan , Liu Xiaochun , Zhu Xiangyu , Wang Shudao , Zhu Huihui , Cao Peipei , Liu Xiongjun , Zhang Xiaobin , Wang Hui , Jiang Suihe , Lu Zhaoping
Published: March 2023

Oxygen solute strengthening is an effective strategy to harden alloys, yet, it often deteriorates the ductility. Ordered oxygen complexes (OOCs), a state between random interstitials and oxides, can simultaneously enhance strength and ductility in high-entropy alloys. However, whether this particular strengthening mechanism holds in other alloys and how these OOCs are tailored remain unclear. Herein, we demonstrate that OOCs can be obtained in bcc (body-centered-cubic) Ti-Zr-Nb medium-entropy alloys via adjusting the content of Nb and oxygen. Decreasing the phase stability enhances the degree of (Ti, Zr)-rich chemical short-range orderings, and then favors formation of OOCs after doping oxygen. Moreover, the number density of OOCs increases with oxygen contents in a given alloy, but adding excessive oxygen (>3.0 at.%) causes grain boundary segregation. Consequently, the tensile yield strength is enhanced by ~75% and ductility is substantially improved by ~164% with addition of 3.0 at.% O in the Ti-30Zr-14Nb MEA.
Safety and immunogenicity of a thermostable ID93 + GLA-SE tuberculosis vaccine candidate in healthy adults
Authors: Sagawa Zachary K. , Goman Cristina , Frevol Aude , Blazevic Azra , Tennant Janice , Fisher Bridget , Day Tracey , Jackson Stephen , Lemiale Franck , Toussaint Leon , Kalisz Irene , Jiang Joe , Ondrejcek Lisa , Mohamath Raodoh , Vergara Julie , Lew Alan , Beckmann Anna Marie , Casper Corey , Hoft Daniel F. , Fox Christopher B.
Published: March 2023

Adjuvant-containing subunit vaccines represent a promising approach for protection against tuberculosis (TB), but current candidates require refrigerated storage. Here we present results from a randomized, double-blinded Phase 1 clinical trial (NCT03722472) evaluating the safety, tolerability, and immunogenicity of a thermostable lyophilized single-vial presentation of the ID93 + GLA-SE vaccine candidate compared to the non-thermostable two-vial vaccine presentation in healthy adults. Participants were monitored for primary, secondary, and exploratory endpoints following intramuscular administration of two vaccine doses 56 days apart. Primary endpoints included local and systemic reactogenicity and adverse events. Secondary endpoints included antigen-specific antibody (IgG) and cellular immune responses (cytokine-producing peripheral blood mononuclear cells and T cells). Both vaccine presentations are safe and well tolerated and elicit robust antigen-specific serum antibody and Th1-type cellular immune responses. Compared to the non-thermostable presentation, the thermostable vaccine formulation generates greater serum antibody responses (p < 0.05) and more antibody-secreting cells (p < 0.05). In this work, we show the thermostable ID93 + GLA-SE vaccine candidate is safe and immunogenic in healthy adults.
Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans
Authors: Hou Xinhao , Xu Mingjing , Zhu Chengming , Gao Jianing , Li Meili , Chen Xiangyang , Sun Cheng , Nashan Björn , Zang Jianye , Zhou Ying , Guang Shouhong , Feng Xuezhu
Published: March 2023

The chromatin organization modifier domain (chromodomain) is an evolutionally conserved motif across eukaryotic species. The chromodomain mainly functions as a histone methyl-lysine reader to modulate gene expression, chromatin spatial conformation and genome stability. Mutations or aberrant expression of chromodomain proteins can result in cancer and other human diseases. Here, we systematically tag chromodomain proteins with green fluorescent protein (GFP) using CRISPR/Cas9 technology in C. elegans. By combining ChIP-seq analysis and imaging, we delineate a comprehensive expression and functional map of chromodomain proteins. We then conduct a candidate-based RNAi screening and identify factors that regulate the expression and subcellular localization of the chromodomain proteins. Specifically, we reveal an H3K9me1/2 reader, CEC-5, both by in vitro biochemistry and in vivo ChIP assays. MET-2, an H3K9me1/2 writer, is required for CEC-5 association with heterochromatin. Both MET-2 and CEC-5 are required for the normal lifespan of C. elegans. Furthermore, a forward genetic screening identifies a conserved Arginine124 of CEC-5’s chromodomain, which is essential for CEC-5’s association with chromatin and life span regulation. Thus, our work will serve as a reference to explore chromodomain functions and regulation in C. elegans and allow potential applications in aging-related human diseases.
Single-vat single-cure grayscale digital light processing 3D printing of materials with large property difference and high stretchability
Authors: Yue Liang , Macrae Montgomery S. , Sun Xiaohao , Yu Luxia , Song Yuyang , Nomura Tsuyoshi , Tanaka Masato , Jerry Qi H.
Published: March 2023

Multimaterial additive manufacturing has important applications in various emerging fields. However, it is very challenging due to material and printing technology limitations. Here, we present a resin design strategy that can be used for single-vat single-cure grayscale digital light processing (g-DLP) 3D printing where light intensity can locally control the conversion of monomers to form from a highly stretchable soft organogel to a stiff thermoset within in a single layer of printing. The high modulus contrast and high stretchability can be realized simultaneously in a monolithic structure at a high printing speed (z-direction height 1 mm/min). We further demonstrate that the capability can enable previously unachievable or hard-to-achieve 3D printed structures for biomimetic designs, inflatable soft robots and actuators, and soft stretchable electronics. This resin design strategy thus provides a material solution in multimaterial additive manufacture for a variety of emerging applications.
Estimating immunity with mathematical models for SARS-CoV-2 after COVID-19 vaccination
Authors: Uwamino Yoshifumi , Nagashima Kengo , Yoshifuji Ayumi , Suga Shigeru , Nagao Mizuho , Fujisawa Takao , Ryuzaki Munekazu , Takemoto Yoshiaki , Namkoong Ho , Wakui Masatoshi , Matsushita Hiromichi , Hasegawa Naoki , Sato Yasunori , Murata Mitsuru
Published: March 2023

Tools that can be used to estimate antibody waning following COVID-19 vaccinations can facilitate an understanding of the current immune status of the population. In this study, a two-compartment-based mathematical model is formulated to describe the dynamics of the anti-SARS-CoV-2 antibody in healthy adults using serially measured waning antibody concentration data obtained in a prospective cohort study of 673 healthcare providers vaccinated with two doses of BNT162b2 vaccine. The datasets of 165 healthcare providers and 292 elderly patients with or without hemodialysis were used for external validation. Internal validation of the model demonstrated 97.0% accuracy, and external validation of the datasets of healthcare workers, hemodialysis patients, and nondialysis patients demonstrated 98.2%, 83.3%, and 83.8% accuracy, respectively. The internal and external validations demonstrated that this model also fits the data of various populations with or without underlying illnesses. Furthermore, using this model, we developed a smart device application that can rapidly calculate the timing of negative seroconversion.
Rupturing aromaticity by periphery overcrowding
Authors: Saha Promeet K. , Mallick Abhijit , Turley Andrew T. , Bismillah Aisha N. , Danos Andrew , Monkman Andrew P. , Avestro Alyssa-Jennifer , Yufit Dmitry S. , McGonigal Paul R.
Published: March 2023

The balance between strain relief and aromatic stabilization dictates the form and function of non-planar π-aromatics. Overcrowded systems are known to undergo geometric deformations, but the energetically favourable π-electron delocalization of their aromatic ring(s) is typically preserved. In this study we incremented the strain energy of an aromatic system beyond its aromatic stabilization energy, causing it to rearrange and its aromaticity to be ruptured. We noted that increasing the steric bulk around the periphery of π-extended tropylium rings leads them to deviate from planarity to form contorted conformations in which aromatic stabilization and strain are close in energy. Under increasing strain, the aromatic π-electron delocalization of the system is broken, leading to the formation of a non-aromatic, bicyclic analogue referred to as ‘Dewar tropylium’. The aromatic and non-aromatic isomers have been found to exist in rapid equilibrium with one another. This investigation demarcates the extent of steric deformation tolerated by an aromatic carbocycle and thus provides direct experimental insights into the fundamental nature of aromaticity.
Future warming from global food consumption
Authors: Ivanovich Catherine C. , Sun Tianyi , Gordon Doria R. , Ocko Ilissa B.
Published: March 2023

Food consumption is a major source of greenhouse gas (GHG) emissions, and evaluating its future warming impact is crucial for guiding climate mitigation action. However, the lack of granularity in reporting food item emissions and the widespread use of oversimplified metrics such as CO2 equivalents have complicated interpretation. We resolve these challenges by developing a global food consumption GHG emissions inventory separated by individual gas species and employing a reduced-complexity climate model, evaluating the associated future warming contribution and potential benefits from certain mitigation measures. We find that global food consumption alone could add nearly 1 °C to warming by 2100. Seventy five percent of this warming is driven by foods that are high sources of methane (ruminant meat, dairy and rice). However, over 55% of anticipated warming can be avoided from simultaneous improvements to production practices, the universal adoption of a healthy diet and consumer- and retail-level food waste reductions.
Accuracy and social motivations shape judgements of (mis)information
Authors: Rathje Steve , Roozenbeek Jon , Van Bavel Jay J. , van der Linden Sander
Published: March 2023

The extent to which belief in (mis)information reflects lack of knowledge versus a lack of motivation to be accurate is unclear. Here, across four experiments (n = 3,364), we motivated US participants to be accurate by providing financial incentives for correct responses about the veracity of true and false political news headlines. Financial incentives improved accuracy and reduced partisan bias in judgements of headlines by about 30%, primarily by increasing the perceived accuracy of true news from the opposing party (d = 0.47). Incentivizing people to identify news that would be liked by their political allies, however, decreased accuracy. Replicating prior work, conservatives were less accurate at discerning true from false headlines than liberals, yet incentives closed the gap in accuracy between conservatives and liberals by 52%. A non-financial accuracy motivation intervention was also effective, suggesting that motivation-based interventions are scalable. Altogether, these results suggest that a substantial portion of people’s judgements of the accuracy of news reflects motivational factors.
Puddle formation and persistent gaps across the non-mean-field breakdown of superconductivity in overdoped (Pb,Bi)2Sr2CuO6+δ
Authors: Tromp Willem O. , Benschop Tjerk , Ge Jian-Feng , Battisti Irene , Bastiaans Koen M. , Chatzopoulos Damianos , Vervloet Amber H. M. , Smit Steef , van Heumen Erik , Golden Mark S. , Huang Yinkai , Kondo Takeshi , Takeuchi Tsunehiro , Yin Yi , Hoffman Jennifer E. , Sulangi Miguel Antonio , Zaanen Jan , Allan Milan P.
Published: March 2023

The cuprate high-temperature superconductors exhibit many unexplained electronic phases, but the superconductivity at high doping is often believed to be governed by conventional mean-field Bardeen–Cooper–Schrieffer theory1. However, it was shown that the superfluid density vanishes when the transition temperature goes to zero2,3, in contradiction to expectations from Bardeen–Cooper–Schrieffer theory. Our scanning tunnelling spectroscopy measurements in the overdoped regime of the (Pb,Bi)2Sr2CuO6+δ high-temperature superconductor show that this is due to the emergence of nanoscale superconducting puddles in a metallic matrix4,5. Our measurements further reveal that this puddling is driven by gap filling instead of gap closing. The important implication is that it is not a diminishing pairing interaction that causes the breakdown of superconductivity. Unexpectedly, the measured gap-to-filling correlation also reveals that pair breaking by disorder does not play a dominant role and that the mechanism of superconductivity in overdoped cuprate superconductors is qualitatively different from conventional mean-field theory.
Evinacumab in severe hypertriglyceridemia with or without lipoprotein lipase pathway mutations: a phase 2 randomized trial
Authors: Rosenson Robert S. , Gaudet Daniel , Ballantyne Christie M. , Baum Seth J. , Bergeron Jean , Kershaw Erin E. , Moriarty Patrick M. , Rubba Paolo , Whitcomb David C. , Banerjee Poulabi , Gewitz Andrew , Gonzaga-Jauregui Claudia , McGinniss Jennifer , Ponda Manish P. , Pordy Robert , Zhao Jian , Rader Daniel J.
Published: March 2023

Severe hypertriglyceridemia (sHTG) is an established risk factor for acute pancreatitis. Current therapeutic approaches for sHTG are often insufficient to reduce triglycerides and prevent acute pancreatitis. This phase 2 trial (NCT03452228) evaluated evinacumab (angiopoietin-like 3 inhibitor) in three cohorts of patients with sHTG: cohort 1, familial chylomicronemia syndrome with bi-allelic loss-of-function lipoprotein lipase (LPL) pathway mutations (n = 17); cohort 2, multifactorial chylomicronemia syndrome with heterozygous loss-of-function LPL pathway mutations (n = 15); and cohort 3, multifactorial chylomicronemia syndrome without LPL pathway mutations (n = 19). Fifty-one patients (males, n = 27; females, n = 24) with a history of hospitalization for acute pancreatitis were randomized 2:1 to intravenous evinacumab 15 mg kg−1 or placebo every 4 weeks over a 12-week double-blind treatment period, followed by a 12-week single-blind treatment period. The primary end point was the mean percent reduction in triglycerides from baseline after 12 weeks of evinacumab exposure in cohort 3. Evinacumab reduced triglycerides in cohort 3 by a mean (s.e.m.) of −27.1% (37.4) (95% confidence interval −71.2 to 84.6), but the prespecified primary end point was not met. No notable differences in adverse events between evinacumab and placebo treatment groups were seen during the double-blind treatment period. Although the primary end point of a reduction in triglycerides did not meet the prespecified significance level, the observed safety and changes in lipid and lipoprotein levels support the further evaluation of evinacumab in larger trials of patients with sHTG. Trial registration number: ClinicalTrials.gov NCT03452228.
Protein interactors of 3-O sulfated heparan sulfates in human MCI and age-matched control cerebrospinal fluid
Authors: Ferreira Andreia , Timmerman Evy , Staes An , Vuylsteke Marnik , De Muynck Louis , Gevaert Kris
Published: March 2023

Heparan sulfates (HS) proteoglycans are commonly found on the cell surface and mediate many processes. Binding of HS ligands is determined by the sulfation code on the HS chain that can be N-/2-O/6-O- or 3-O-sulfated, generating heterogenous sulfation patterns. 3-O sulfated HS (3S-HS) play a role in several (patho)physiological processes such as blood coagulation, viral pathogenesis and binding and internalization of tau in Alzheimer’s disease. However, few 3S-HS-specific interactors are known. Thus, our insight into the role of 3S-HS in health and disease is limited, especially in the central nervous system. Using human CSF, we determined the interactome of synthetic HS with defined sulfation patterns. Our affinity-enrichment mass spectrometry studies expand the repertoire of proteins that may interact with (3S-)HS. Validating our approach, ATIII, a known 3S-HS interactor, was found to require GlcA-GlcNS6S3S for binding, similar to what has been reported. Our dataset holds novel, potential HS and 3S-HS protein ligands, that can be explored in future studies focusing on molecular mechanisms that depend on 3S-HS in (patho)physiological conditions.
Association between triglyceride to high-density lipoprotein cholesterol ratio and type 2 diabetes risk in Japanese
Authors: Wang Huijuan , Wang Changming , Xuan Xiuping , Xie Zhouni , Qiu Yuanyuan , Qin Huiping , Xiaoning Zhong
Published: March 2023

Abnormal lipid metabolism is known to increases the risk for metabolic diseases, such as type 2 diabetes mellitus(T2DM). The relationship between baseline ratio of triglyceride to HDL cholesterol (TG/HDL-C) and T2DM in Japanese adults was investigated in this study. Our secondary analysis included 8419 male and 7034 female Japanese subjects who were free of diabetes at baseline. The correlation between baseline TG/HDL-C and T2DM was analyzed by a proportional risk regression model, the nonlinear correlation between baseline TG/HDL-C and T2DM was analyzed by a generalized additive model (GAM), and the threshold effect analysis was performed by a segmented regression model. We conducted subgroup analyses in different populations. During the median 5.39 years follow-up, 373 participants, 286 males and 87 females, developed diabetes mellitus. After full adjustment for confounders, the baseline TG/HDL-C ratio positively correlated with the risk of diabetes (hazard ratio 1.19, 95% confidence interval 1.09–1.3), and smoothed curve fitting and two-stage linear regression analysis revealed a J-shaped relationship between baseline TG/HDL-C and T2DM. The inflection point for baseline TG/HDL-C was 0.35. baseline TG/HDL-C > 0.35 was positively associated with the development of T2DM (hazard ratio 1.2, 95% confidence interval 1.10–1.31). Subgroup analysis showed no significant differences in the effect between TG/HDL-C and T2DM in different populations. A J-shaped relationship was observed between baseline TG/HDL-C and T2DM risk in the Japanese population. When TG/HDL-C was higher than 0.35, there was a positive relationship between baseline TG/HDL-C and the incidence of diabetes mellitus.
Comparative effects of desflurane and sevoflurane on intraoperative peripheral perfusion index: a retrospective, propensity score matched, cohort study
Authors: Oh Chahyun , Lee Seounghun , Song Byong-Sop , Kwon Sanghun , Kim Yoon-Hee , Yoon Seok-Hwa , Shin Yong Sup , Ko Youngkwon , Lim Chaeseong , Hong Boohwi
Published: March 2023

Desflurane is known to have a larger vasodilatory effect than that of sevoflurane. However, its generalizability and effect size in actual clinical practice are yet to be proven. Patients aged ≥ 18 years who underwent noncardiac surgery under general anesthesia using inhalation anesthetics (desflurane or sevoflurane) were matched 1:1 by propensity score. The mean intraoperative perfusion index (PI) of each patient were compared between the two groups. Propensity score matching of 1680 patients in the study cohort identified 230 pairs of patients. PI was significantly higher in the desflurane group (median of paired difference, 0.45; 95% CI 0.16 to 0.74, p = 0.002). PI durations below 1.0 and 1.5 were significantly longer in the sevoflurane group. Mean arterial pressure (MAP) and durations of low MAP did not differ significantly between the two groups. Generalized linear mixed models revealed that the use of sevoflurane, mean MAP, mean heart rate, age, and duration of anesthesia had significant negative effects (lower PI), whereas mean age-adjusted minimum alveolar concentration of inhalation agent had a positive effect on PI (higher value). Intraoperative PI was significantly higher in patients administered desflurane than sevoflurane. However, the impact of the choice between desflurane and sevoflurane on intraoperative PI in this clinical setting was minimal.
Assessment of the variability of the morphological traits and differentiation of Cucurbita moschata in Cote d’Ivoire
Authors: Seka Dagou , Kouago Badié Arnaud , Bonny Beket Severin
Published: March 2023

With its predisposition to adapt to different environments, Cucurbita moschata grows well in various ecosystems. It is not a very exigent plant and has an inherent capacity for plasticity that underlies its large variability. An assessment of the accessions of C. moschata in Cote d’Ivoire shows that the plant exhibits large morphological and phenological variability for all the 28 measured traits. There are outliers among most measured traits. Further analysis indicates the emergence of three ecotypes in congruence with the three distinct ecosystems and their respective bioclimatic characteristics. In the savannah region, characterized by a short rainy season followed by a long dry season, a total yearly rainfall of 900 mm, an elevated daily temperature of 29 °C, and a high relative humidity of 80%, the cline of C. moschata is long and thin, with small leaves, small peduncles and small fruits. It has a high growth rate and accelerated phenology. The mountain region has a very long rainy season followed by a short dry season, a total pluviometry of 1400 mm, an average daily temperature of 27 °C and a relative humidity of 69%. The cline of C. moschata in the mountain region is characterized by a delayed flowering and a delayed fruit maturity, large number of small seeds and large fruits. The forest region has a favorable climate for the growth of C. moschata in Cote d’Ivoire. It has two rainy seasons that alternate with two dry seasons of unequal durations, an annual rainfall of 1200 mm, an average daily temperature of 27 °C and a relative humidity of 70%. The cline of C. moschata in that region has a large girth size, large dimensions of the leaves, long peduncles and larger and heavier fruits. The seeds are also large but in small number. It appears that the anatomy and physiology of the clines are differentiated primarily in response to soil water content and availability for the ontogeny of the plant.
First record of Jacobsoniidae (Coleoptera) on the African continent in Holocene copal from Tanzania: biogeography since the Cretaceous
Authors: Peris David , Hammel Jörg U. , Cai Chenyang , Solórzano-Kraemer Mónica M.
Published: March 2023

Neither fossil nor living Jacobsoniidae are found in abundance. Derolathrus cavernicolus Peck, 2010 is recorded here preserved in Holocene copal from Tanzania with an age of 210 ± 30 BP years. This leads us to three interesting conclusions: (1) This is the first time the family was found on the African continent, extending the family’s distribution range to hitherto unknown localities. Derolathrus cavernicolus in Holocene copal from Tanzania expands the known distribution of the species, previously only recorded in the USA (Hawaii and Florida), Barbados, and Japan, both spatially and temporally. (2) All fossil specimens of the family have been found preserved in amber, which might be due to the small size of the specimens that prevents their discovery in other types of deposits. However, we here add a second aspect, namely the occurrence of this cryptic and currently scarce family of beetles in resinous environments, where they live in relationship with resin-producing trees. (3) The discovery of a new specimen from a family unknown on the African continent supports the relevance of these younger resins in preserving arthropods that lived in pre-Anthropocene times. Although we cannot demonstrate their extinction in the region, since it is possible that the family still survives in the already fragmented coastal forests of East Africa, we are detecting a loss of local biodiversity during the so-called Anthropocene, probably due to human activity.
Myofascial force transmission between the calf and the dorsal thigh is dependent on knee angle: an ultrasound study
Authors: Mohr Lisa , Vogt Lutz , Thiel Christian , Behringer Michael , Wilke Jan
Published: March 2023

A recent in-vivo experiment has shown that force can be transmitted between the gastrocnemius and the hamstring muscles due to a direct tissue continuity. However, it remains unclear if this mechanical interaction is affected by the stiffness of the structural connection. This study therefore aimed to investigate the impact of the knee angle on myofascial force transmission across the dorsal knee. A randomized, cross-over study was performed, including n = 56 healthy participants (25.36 ± 3.9 years, 25 females). On two separate days, they adopted a prone position on an isokinetic dynamometer (knee extended or 60° flexed). In each condition, the device moved the ankle three times from maximal plantarflexion to maximal dorsal extension. Muscle inactivity was ensured using EMG. High-resolution ultrasound videos of the semimembranosus (SM) and the gastrocnemius medialis (GM) soft tissue were recorded. Maximal horizontal tissue displacement, obtained using cross-correlation, was examined as a surrogate of force transmission. SM tissue displacement was higher at extended (4.83 ± 2.04 mm) than at flexed knees (3.81 ± 2.36 mm). Linear regression demonstrated significant associations between (1) SM and GM soft tissue displacement (extended: R2 = 0.18, p = 0.001; flexed: R2 = 0.17, p = 0.002) as well as (2) SM soft tissue displacement and ankle range of motion (extended: R2 = 0.103, p = 0.017; flexed: R2 = 0.095, p = 0.022). Our results further strengthen the evidence that local stretching induces a force transmission to neighboring muscles. Resulting remote exercise effects such as increased range of motion, seem to depend on the stiffness of the continuity.

Trial registration: DRKS (Deutsches Register Klinischer Studien), registration number DRKS00024420, first registered 08/02/2021, https://drks.de/search/de/trial/DRKS00024420.