bluuebunny/crossref_metadata_2025 huggingface dataset:
A GRASP-Based Approach for Planning UAV-Assisted Search and Rescue Missions
Authors: Pedersen Casper Bak , Nielsen Kasper Gaj , Rosenkrands Kasper , Vasegaard Alex Elkjær , Nielsen Peter , El Yafrani Mohamed
Published: December 2021

Search and Rescue (SAR) missions aim to search and provide first aid to persons in distress or danger. Due to the urgency of these situations, it is important to possess a system able to take fast action and effectively and efficiently utilise the available resources to conduct the mission. In addition, the potential complexity of the search such as the ruggedness of terrain or large size of the search region should be considered. Such issues can be tackled by using Unmanned Aerial Vehicles (UAVs) equipped with optical sensors. This can ensure the efficiency in terms of speed, coverage and flexibility required to conduct this type of time-sensitive missions. This paper centres on designing a fast solution approach for planning UAV-assisted SAR missions. The challenge is to cover an area where targets (people in distress after a hurricane or earthquake, lost vessels in sea, missing persons in mountainous area, etc.) can be potentially found with a variable likelihood. The search area is modelled using a scoring map to support the choice of the search sub-areas, where the scores represent the likelihood of finding a target. The goal of this paper is to propose a heuristic approach to automate the search process using scarce heterogeneous resources in the most efficient manner.
A Hybrid Taguchi-Regression Algorithm for a Fuel Injection Control System
Authors: Tsai Wen-Chang
Published: December 2021

The fuel injection system is one of the key components of an in-cylinder direct injection engine. Its performance directly affects the economy, power and emission of the engine. Previous research found that the Taguchi method can be used to optimize the fuel injection map and operation parameters of the injection system. The electronic control injector was able to steadily control the operation performance of a high-pressure fuel injection system, but its control was not accurate enough. This paper conducts an experimental analysis for the fuel injection quantity of DI injectors using the Taguchi-Regression approach, and provides a decision-making analysis to improve the design of electronic elements for the driving circuit. In order to develop a more stable and energy-saving driver, a functional experiment was carried out. The hybrid Taguchi-regression algorithm for injection quantity of a direct injection injector was examined to verify the feasibility of the proposed algorithm. This paper also introduces the development of a high-pressure fuel injection system and provides a new theoretical basis for optimizing the performance of an in-cylinder gasoline direct injection engine. Finally, a simulation study for the fuel injection control system was carried out under the environment of MATLAB/Simulink to validate the theoretical concepts.
Virtualizing Project-Based Learning: An Abrupt Adaptation of Active Learning in the First Days of the COVID-19 Pandemic, with Promising Outcomes
Authors: Pereira Marco Antonio Carvalho , Ignácio Luísa Miranda Nunes da Costa , Rodrigues Reis Cristiano E.
Published: December 2021

Project-based learning (PBL) is renowned as an active learning practice that promotes the application of the accumulation of knowledge through real-world and often open-ended problems. This work assesses a case evaluation of an introductory PBL course in a typical industrial engineering curriculum in Brazil. This course is taken during the first semester, in which students must develop solutions for a single given problem through weekly meetings. The case presented herein highlights the forced abrupt virtualization of the learning process, which imposed an unprecedented scenario on the students and instructors, especially with regard to this course, which is based primarily on presented discussions and activities. The first weeks of class following the abrupt virtualization of activities encountered misinformation and a lack of clarity about the adaptation of the activities. Fortunately, through rapid iterations, the adjustment process resulted in time invested by the students and classes, with an active discussion, using the tools made available by the university. This work aims to present the forced abrupt changes applied to this first-semester course, highlighting the challenges faced, and the positive outcomes obtained and observed by both the students and the instructor, and to make a comparison to the evolution of the course over the past years.
Challenge of Using Groundwater for Buildings Air Conditioning in Subtropical Areas
Authors: Kuo Chih-Ping , Liao Hung-Jiun
Published: December 2021

Using circulating groundwater to cool air-conditioning is not new in high latitude regions but difficult in subtropical areas. Different from only using fans to remove the heat from indoor air for drier air in the high latitude region, the latent heat inside the humid air in subtropical areas makes the operation more difficult. Latent heat inside the humid air must remove away by air-conditioning including compressor and fan for cooling indoor air, which means more electrical power is required for the operation. To save total electrical power for the air-conditioning system is the main goal of this study. To use the advantage of groundwater with lower temperature to lower down the work of compressor, this research compared two ways, close/open types of water/groundwater circulation, both using groundwater to remove the heat generated by a 15RT (45 kW) air-conditioning. Full-scale tests and simulations were performed in this study to evaluate the efficiency of transferring the heat produced by air-conditioning systems to stably flowing groundwater in a grave stratum under Taipei Basin. With a closed circulating cooling water system, this study found that a 15RT air conditioner could only operate continuously for 4 h before it had to be shut down due to overheating. Additionally, groundwater must carry the heat away within the following 20 h. In changing the closed circulating water system to an open one, a system that uses a circulatory method to extract groundwater upwards and conduct heat exchange with an air conditioning system can enable the continuous operation of such a system with the same heat production condition. Numerical simulations for the heat dissipation behavior of two circulatory systems were performed herein. The results verified the aforementioned phenomena observed from both tests. The result showed both systems can provide air-conditioning working well. The total electrical power for a 15RT air-conditioning in sub-tropical areas can be reduced by 22% using circulating groundwater. Considering the system optimization, the total power consumption can be reduced by about 28%.
The Potential for Hydrolysed Sheep Wool as a Sustainable Source of Fertiliser for Irish Agriculture
Authors: Gillespie Gary D. , Dada Oyinlola , McDonnell Kevin P.
Published: December 2021

Suppressed wool prices in Ireland over the last number of years has led to situations where the cost of shearing animals is greater than the wools’ value, leading to net losses per animal for farmers. Populations of sheep in Ireland and nutrient values of wool from literature sources were used to determine the quantity of nutrients that could be produced on an annual basis using hydrolysis techniques. Results of this study suggest that up to 15.8% of the nitrogen required to produce Ireland’s cereal crops can be met annually using hydrolysed sheep wool in an economically feasible manner along with considerable amounts of sulphur, zinc, and copper. Most of the cost associated with the process is the purchasing of wool from farmers at an economically favourable level for farmers. Based on the spatial distribution of these animals, the town of Athlone is the most suitable location for a processing facility.
Why Do Companies Need Operational Flexibility to Reduce Waste at Source?
Authors: Elalem Yara Kayyali , Bicer Isik , Seifert Ralf W.
Published: December 2021

We analyze the environmental benefits of operational flexibility that emerge in the form of less product waste during the sourcing process by reducing overproduction. We consider three different options for operational flexibility: (1) lead-time reduction, (2) quantity-flexibility contracts, and (3) multiple sourcing. We use a multiplicative demand process to model the evolutionary dynamics of demand uncertainty. We then quantify the impact of key modeling parameters for each operational-flexibility strategy on the waste ratio, which is measured as the ratio of excess inventory when a certain operational-flexibility strategy is employed to the amount when an offshore supplier is utilized without any operational flexibility. We find that the lead-time reduction strategy has the maximum capability to reduce waste in the sourcing process of buyers, followed by the quantity-flexibility and multiple-sourcing strategies, respectively. Thus, our results indicate that operational-flexibility strategies that rely on the localization of production are key to reducing waste and improving environmental sustainability at source.
Combination of Coagulation, Adsorption, and Ultrafiltration Processes for Organic Matter Removal from Peat Water
Authors: Elma Muthia , Pratiwi Amalia Enggar , Rahma Aulia , Rampun Erdina Lulu Atika , Mahmud Mahmud , Abdi Chairul , Rosadi Raissa , Yanto Dede Heri Yuli , Bilad Muhammad Roil
Published: December 2021

The high content of natural organic matter (NOM) is one of the challenging characteristics of peat water. It is also highly contaminated and contributes to some water-borne diseases. Before being used for potable purposes, peat water must undergo a series of treatments, particularly for NOM removal. This study investigated the effect of coagulation using aluminum sulfate coagulant and adsorption using powdered activated carbon (PAC) as a pretreatment of ultrafiltration (UF) for removal of NOM from actual peat water. After preparation and characterization of polysulfone (Psf)-based membrane, the system’s performance was evaluated using actual peat water, particularly on NOM removal and the UF performances. The coagulation and adsorption tests were done under variable dosings. Results show that pretreatment through coagulation–adsorption successfully removed most of the NOM. As such, the UF fouling propensity of the pretreated peat water was substantially lowered. The optimum aluminum sulfate dosing of 175 mg/L as the first pretreatment stage removed up to 75–78% NOM. Further treatment using the PAC-based adsorption process further increased 92–96% NOM removals at an optimum PAC dosing of 120 mg/L. The final UF-PSf treatment reached NOM removals of 95% with high filtration fluxes of up to 92.4 L/(m2.h). The combination of three treatment stages showed enhanced UF performance thanks to partial pre-removal of NOM that otherwise might cause severe membrane fouling.
Multi-Objective Spatial Suitability Evaluation and Conflict Optimization Considering Productivity, Sustainability, and Livability in Southwestern Mountainous Areas of China
Authors: Fang Yishu , Ai Dong , Yang Yuting , Sun Weijian , Zu Jian
Published: December 2021

Space is the fundamental carrier for production, living, and ecological activities, and optimizing the spatial pattern is of vital importance to promote regional sustainable development. To achieve this goal, the core issues are to identify the risks of resource and environmental constraints of development and to realize the rational distribution of human living space. Based on the integration of multisource heterogeneous data, taking Yunnan Province, a typical mountainous area in China, as an example, this research proposes a multi-object suitability evaluation method based on 50 × 50 m grid data at the provincial scale. We build a spatial conflict analysis model to identify production–living–ecological space (PLES) and propose governance suggestions for different functional areas. The results show that (1) areas suitable for ecology make up the greatest proportion of Yunnan Province, but areas with living and ecological functions show obvious spatial complementarity; (2) areas suitable for production are restricted by steep slope, geological hazards and fragmented pattern; (3) areas suitable for living is rare, and they are mainly concentrated in the plains of central Yunnan; and (4) twenty-seven percent of area has potential spatial conflicts, among which 4.38% of the area is all suitable for production–living–ecological. The production–living advantage areas are concentrated in the central Yunnan UA (Urban agglomeration), which has a high spatial overlap. These results are expected to provide valuable insights to support comprehensive multifunctional spatial utilization and sustainable development in mountainous areas.
Evaluating the Efficacy of Sustainability Initiatives in the Canadian Port Sector
Authors: MacNeil Jennifer L. , Adams Michelle , Walker Tony R.
Published: December 2021

Maritime ports are critical nodes in the Canadian resource-based economy that can have significant environmental impacts near coastal communities and marine ecosystems. To address these impacts, Canadian Port Authorities (CPAs) assess their environmental performance using the Green Marine Environmental Program (GMEP). Reliance on this program necessitates its evaluation as an effective initiative to address sustainability in its broader context. An analysis was performed to identify links between United Nations Sustainable Development Goals (UN SDG) targets relevant to the Canadian Port Sector and GMEP performance indicators. Results indicate that there are significant gaps in the GMEP, with only 14 of 36 relevant SDG targets directly linked to the program. Findings suggest either an expansion of the GMEP to incorporate these broader sustainability goals, or the development and inclusion of a new framework for CPAs to bridge gaps between the GMEP and SDG targets to improve sustainability in their maritime port operations.
Treatment Technologies for Cooling Water Blowdown: A Critical Review
Authors: Soliman Mariam , Eljack Fadwa , Kazi Monzure-Khoda , Almomani Fares , Ahmed Elalim , El Jack Ziad
Published: December 2021

Cooling water blowdown (CWBD) generated from different industries and district cooling facilities contains high concentrations of various chemicals (e.g., scale and corrosion inhibitors) and pollutants. These contaminants in CWBD streams deem them unsuitable for discharge into surface water and some wastewater treatment plants. The pollutants present in CWBD, their sources, and the corresponding impacts on the ecosystem are discussed. The international and regional (Gulf states) policies and regulations related to contaminated water discharge standards into water bodies are examined. This paper presents a comprehensive review of the existing and emerging water treatment technologies for the treatment of CWBD. The study presents a comparison between the membrane (membrane distillation (MD), reverse osmosis (RO), nanofiltration (NF), and vibratory shear enhanced membrane process (VSEP)) and nonmembrane-based (electrocoagulation (EC), ballasted sand flocculation (BSF), and electrodialysis (ED)) technologies on the basis of performance, cost, and limitations, along with other factors. Results from the literature revealed that EC and VSEP technologies generate high treatment performance (EC~99.54% reduction in terms of silica ions) compared to other processes (membrane UF with reduction of 65% of colloidal silica). However, the high energy demand of these processes (EC~0.18–3.05 kWh/m3 and VSEP~2.1 kWh/m3) limit their large-scale applications unless connected with renewable sources of energy.
Bidirectional Interface Resonant Converter for Wide Voltage Range Storage Applications
Authors: Arazi Mouncif , Payman Alireza , Camara Mamadou Baïlo , Dakyo Brayima
Published: December 2021

In this paper, a bidirectional zero voltage switching (ZVS) resonant converter with narrow control frequency deviation is proposed. Wide input–output voltage range applications, such as flywheel or supercapacitors storage units are targeted. Due to symmetrical topology of resonant circuit interfaces, the proposed converter has similar behavior in bidirectional operating mode. We call it Dual Active Bridge Converter (DABC). The proposal topology of the converter is subjected to multi resonant circuits which make it necessary to study with multiscale approaches. Thus, first harmonic approximation and use of selective per unit parameters are established in (2) Methods. Then, the forward direction and backward direction of power flux exchange are detailed according to switching sequences. Switching frequency control must be completed within a narrow range. So, the frequency range deterministic parameters are emphasized in the design procedure in (3) Methods. A narrow range of switching frequency and a wide range voltage control must be ensured to suit for energy storage units, power electronic devices capabilities and electromagnetic compatibility. A 3 kW test bench is used to validate operation principles and to proof success of the developed design procedure. The interest of proposed converter is compared to other solutions from the literature in (4) Results.
Can a Science, Technology, Engineering, and Mathematics (STEM) Approach Enhance Students’ Mathematics Performance?
Authors: Kong Suik Fern , Mohd Matore Mohd Effendi Ewan
Published: December 2021

The STEM approach is a student-centred teaching and learning process that involves an inquiry process in problem-solving questions. This study was conducted to examine the effectiveness of the STEM approach in enhancing students’ mathematics performance. The study used a quasi-experimental design of unbalanced groups through the pre-test and post-test for treatment and control groups. The instrument included a set of questionnaires on student attitudes towards STEM implementation and the three-dimensional geometric shapes achievement test. The research samples included 14-year-old students from one of the private secondary schools in Kuching, which involved 68 students who were selected using purposive sampling. The results showed that student attitudes towards the implementation of the STEM approach were at a moderate level. In addition, the results illustrated a significant and moderate difference in mathematics performance between students who participated in the STEM approach and the conventional method using pre-test and post-test. The mean score of the post-test performance for the STEM approach was higher compared to the mean score of post-test performance for the conventional method. The results of the study demonstrated that the implementation of the STEM approach that involved both inquiry-based learning and problem-based learning was effective and able to improve the students’ academic performance. This can help teachers to vary their teaching and learning methods by increasing student interactions and engagement. Improvements can be made in the future by adding more research samples to expand the context of the study and prolong the treatment duration. Researchers can also add research variables in future studies by examining student interests and motivations towards the STEM approach.
Pedestrian-Level Wind Environment Assessment of Shenyang’s Residential Areas through Numerical Simulations
Authors: Zhang Jiuhong , Zhang Xiaoqian
Published: December 2021

In recent decades, increasing urbanization has increased construction land shortages, which has made people pay more attention to the utilization of vertical space. The emergence of more and more high-rise buildings has affected the wind environment at the pedestrian level, especially in residential areas. In this research, the typical patterns of the layouts of residential buildings in Shenyang were investigated and summarized, and the wind environment of the residential areas of different architecture layouts was simulated according to the climatic conditions in Shenyang. After analyzing the simulation results, a typical layout mode for the residential areas in Shenyang was developed to facilitate the establishment of a favorable wind environment. In comparison with different building layouts, a staggered layout of slab buildings, half-enclosed layout of point buildings with openings on the south side, slab-point combined buildings with slab buildings on the north side, and point buildings on the south side were found to be the most suitable layouts for Shenyang’s climate. Thus, this study can provide guidance to designers and urban planners in addition to practical suggestions for residential planning.
Assessing the Impact of Climate Resilient Technologies in Minimizing Drought Impacts on Farm Incomes in Drylands
Authors: Samuel Josily , Rao Chitiprolu Anantha Rama , Raju Bellapukonda Murali Krishna , Reddy Anugu Amarender , Pushpanjali  , Reddy Ardha Gopala Krishna , Kumar Ramaraju Nagarjuna , Osman Mohammed , Singh Vinod Kumar , Prasad Jasti Venkata Naga Satya
Published: December 2021

Asia is the region most vulnerable to climate change and India is ranked as one of the most climate vulnerable countries in the world, frequently affected by natural disasters. In this study, we investigated the impact of drought on crop productivity, farmer’s employment and income. The difference-in-difference model (DID) and stepwise multiple linear regression (MLR) were employed to quantify the impact of adopting climate resilient technologies (CRTs) on farm household income during a drought. The factors influencing farm incomes were analyzed using MLR. The study used survey data collected from the drought prone district of Telangana, India. Sixty farmers each from a village adopted under the National Innovations in Climate Resilient Agriculture (NICRA) program and a control village were interviewed. Primary data on the socio-economic characteristic of farmers, cropping pattern, income composition, productivity of major crops, employment and climate resilient interventions adopted by farmers were collected using a well-structured schedule. The results reveal that income crop cultivation was the major contributor to household income (60%) followed by livestock rearing. Farmers reported that droughts decreased the income from crops by 54 per cent and income from livestock rearing by 40 per cent. The farmers belonging to the climate resilient village had 35 per cent higher incomes compared to those in the control village and it was estimated to be Rs. 31,877/farm household/year during droughts using the DID estimate. Farm size, livestock possession, adoption of CRTs and investment in agriculture were the determining factors influencing farm income. Thus, farmers especially in drought prone regions need to be encouraged and supported to adopt cost effective, location specific climate resilient technologies.
Soil Communities: Who Responds and How Quickly to a Change in Agricultural System?
Authors: Coller Emanuela , Oliveira Longa Claudia Maria , Morelli Raffaella , Zanoni Sara , Cersosimo Ippolito Marco Cristiano , Pindo Massimo , Cappelletti Cristina , Ciutti Francesca , Menta Cristina , Zanzotti Roberto , Ioriatti Claudio
Published: December 2021

The use of conservation and sustainable practices could restore the abundance and richness of soil organisms in agroecosystems. Fitting in this context, this study aimed to highlight whether and how different soil living communities reacted to the conversion from an integrated to an organic orchard. The metataxonomic approach for fungi and bacteria and the determination of biological forms of diatoms and microarthropods were applied. Soil analyses were carried out in order to evaluate the effect of soil chemical features on four major soil living communities. Our results showed that the different taxa reacted with different speeds to the management changes. Fungi responded quickly to the changes, suggesting that modification in agricultural practices had a greater impact on fungal communities. Bacteria and microarthropods were more affected by abiotic parameters and less by the management. The diatom composition seemed to be affected by seasonality but the highest H’ (Shannon index) value was measured in the organic system. Fungi, but also diatoms, seemed to be promising for monitoring changes in the soil since they were sensitive to both the soil features and the anthropic impact. Our study showed that soil biodiversity could be affected by the conversion to sustainable management practices from the early years of an orchard onwards. Therefore, better ecological orchard management may strengthen soil sustainability and resilience in historically agricultural regions.
Quartz Mining Waste for Concrete Production: Environment and Public Health
Authors: de Carvalho Frank A. , Nobre Juliana N. P. , Cambraia Rosana P. , Silva Alexandre C. , Fabris José D. , dos Reis Arlete B. , Prat Bernat V.
Published: December 2021

Brazil, one of the largest ore producers in the world, holds 95% of the world’s quartz reserves. The aim of this research is to enhance mitigation measures in quartz ore exploration common in the Serra do Espinhaço Meridional region through the specific study of a mining venture. The three (3) phases of the study were: (1) characterization of the project under study and evaluation of the impacts generated; (2) evaluation of the economic feasibility of using the waste as a coarse aggregate for concrete production; (3) evaluation of the technical feasibility of reusing this waste through analysis of compressive strength. The results of the study show potential negative impacts on occupational health from the piles of disposed waste, specifically silicosis, caused by silica dust dispersed in the air. In the economic analysis, a decrease of 49.05% was verified in coarse aggregate cost through the use of the residue. The compressive strength of the concrete was 26.80 MPa when quartz residue was used and 29.2 MPa when limestone was used. The quartz residue generated by the venture can be reused as aggregate for the production of concrete, generating improvements in environmental and health aspects.
Application of Geoelectrical Survey and Time-Lapse Resistivity with Groundwater Data in Delineating a Groundwater Potential Map: A Case Study from Phuket Island, Thailand
Authors: Puttiwongrak Avirut , Men Ratha , Vann Sakanann , Hashimoto Kiyota , Suteerasak Thongchai
Published: December 2021

Phuket is well-known around the world as a popular tourist destination. Tourism-related population growth depends more on groundwater as the only available source of potable water in Phuket. The proper precautions must be taken to reduce the risk of spending large sums of money in sinking abortive boreholes, and a groundwater potential map would enhance the success rate of future groundwater exploration and exploitation in the study area. Geoelectrical surveys were carried out in this study to collect electrical properties of the subsurface, and the Dar-Zarrouk parameters (DZP) were calculated using the geoelectrical data. The first thematic groundwater potential map was constructed using the interpretation of DZP, while the second thematic groundwater potential map was created using the basis maps of the depth-of-basement and aquifer thickness parameters. Finally, two thematic maps based on geographic information system (GIS) environments were overlaid on a groundwater potential map of Phuket. However, in order to provide a reliable assessment of groundwater potential, time-lapse electrical resistivity imaging was used to confirm the area of the high-potential zone indicated on the map. The map created by this study is aimed to act as a reference for future groundwater exploration and exploitation, preventing water supplies from becoming unsustainable through botched borehole drilling for groundwater production.
Geometric Morphometrics of Bilateral Asymmetry in Eunotia bilunaris (Eunotiales, Bacillariophyceae) as a Tool for the Quantitative Assessment of Teratogenic Deviations in Frustule Shapes
Authors: Woodard Katerina , Neustupa Jiri
Published: December 2021

A number of pennate diatom genera typically have teratogenic deformations of their siliceous frustules due to the effects of environmental stress, such as high concentrations of heavy metals and low pH. However, the quantitative assessment of these deformations has rarely been applied. One species in which aberrations have frequently been reported is Eunotia bilunaris, which typically has bilaterally symmetric frustules with dorso-ventral differentiation. In this study, we aimed to illustrate the geometric morphometric analysis of symmetry as a tool for assessing the severity of teratogenic deformations. These were quantified by Procrustes superimposition of equidistant points placed along the valvar outlines in pairs of configurations based on their bilateral reflection symmetry. The shape deformations were mostly confined to central parts of the ventral outlines and were captured both by the symmetric and asymmetric subspaces of the variation. The amount of bilateral asymmetry in individual cells was negatively related to frustule size via the allometric power law relationship, illustrating that asymmetry increased in the asexual diminution series. The presented analysis provides a framework for the quantitative assessment of frustule deformations in eunotioid diatoms that can be used for the comparative scoring of teratogenic deviations among cells, populations, or species.
Twistor Bundle of a Neutral Kähler Surface
Authors: Jelonek Włodzimierz
Published: December 2021

In this paper, we characterize neutral Kähler surfaces in terms of their positive twistor bundle. We prove that an O+,+(2,2)-oriented four-dimensional neutral semi-Riemannian manifold (M,g) admits a complex structure J with ΩJ∈⋀−M, such that (M,g,J) is a neutral-Kähler manifold if and only if the twistor bundle (Z1(M),gc) admits a vertical Killing vector field.
Tracking and Monitoring of the Alignment System Used for Nuclear Physics Experiments
Authors: Borza Paul Nicolae , Vlase Sorin
Published: December 2021

The ELI-NP (Extreme Light Intensity—Nuclear Physics) project, developed at the Horia Hulubei National Institute for RD in Physics and Nuclear Engineering (IFIN-HH), has included one component dedicated to the study of interactions between brilliant gamma-ray and matter, with applications in nuclear physics and the science of materials. The paper is focused on the interaction chamber, an important part of the facility which hosts the experiment’s samples. The interaction chamber is endowed with a mobile sample support (holder), which automatically tracks the γ-ray beam. The γ-ray radiation source presents a slight variation of the direction of the emitted radiation in time. The built system ensures the permanent collimation between the γ-ray beam and the sample that is being investigated. This is done with two electric motors, which have a symmetrical movement with respect to the center of a rectangle. The specific measures taken by the design and implementation that permit to reach performances of tracking system are emphasized in the paper. The methodology considers the relative displacement between the detectors with which the laboratory is equipped and the absolute position in space of the sample boundary. The control of this motion is designed to respect the symmetry of the system. Both facets of the project (hardware and software) are detailed, emphasizing the way in which the designers ensured compliance with the system of real-time operation conditions of the tracking and monitoring system.
First Description of the Female of the Trinidadian Theraphosine Spinosatibiapalpus trinitatis (Pocock, 1903) (Araneae: Theraphosidae)
Authors: Sherwood Danniella , Guadanucci José Paulo Leite , Gabriel Ray
Published: December 2021

The hitherto unknown female of the theraphosine Spinosatibiapalpus trinitatis (Pocock, 1903) is herein described based on the paralectotype series of the schismatotheline Neoholothele incei (F. O. Pickard-Cambridge, 1899) housed in the collections of the Natural History Museum, London.
Alimentary Infections by Tick-Borne Encephalitis Virus
Authors: Ličková Martina , Fumačová Havlíková Sabína , Sláviková Monika , Klempa Boris
Published: December 2021

Tick-borne encephalitis virus (TBEV) causes serious the neurological disease, tick-borne encephalitis (TBE). TBEV can be transmitted to humans by ticks as well as by the alimentary route, which is mediated through the consumption of raw milk products from infected ruminants such as sheep, goats, and cows. The alimentary route of TBEV was recognized in the early 1950s and many important experimental studies were performed shortly thereafter. Nowadays, alimentary TBEV infections are recognized as a relevant factor contributing to the overall increase in TBE incidences in Europe. This review aims to summarize the history and current extent of alimentary TBEV infections across Europe, to analyze experimental data on virus secretion in milk, and to review possible alimentary infection preventive measures.
Merkel Cell Polyomavirus: Oncogenesis in a Stable Genome
Authors: Ahmed Mona M. , Cushman Camille H. , DeCaprio James A.
Published: December 2021

Merkel cell polyomavirus (MCV) is the causative agent for the majority of Merkel cell carcinoma (MCC) cases. Polyomavirus-associated MCC (MCCP) is characterized by the integration of MCV DNA into the tumor genome and a low tumor mutational burden. In contrast, nonviral MCC (MCCN) is characterized by a high tumor mutational burden induced by UV damage. Since the discovery of MCV, much work in the field has focused on understanding the molecular mechanisms of oncogenesis driven by the MCV tumor (T) antigens. Here, we review our current understanding of how the activities of large T (LT) and small T (ST) promote MCC oncogenesis in the absence of genomic instability. We highlight how both LT and ST inhibit tumor suppressors to evade growth suppression, an important cancer hallmark. We discuss ST interactions with cellular proteins, with an emphasis on those that contribute to sustaining proliferative signaling. Finally, we examine active areas of research into open questions in the field, including the origin of MCC and mechanisms of viral integration.
Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori
Authors: Liu Yujia , Chen Dongbin , Zhang Xiaoqian , Chen Shuqing , Yang Dehong , Tang Linmeng , Yang Xu , Wang Yaohui , Luo Xingyu , Wang Manli , Hu Zhihong , Huang Yongping
Published: December 2021

The silkworm Bombyx mori is an economically important insect. The sericulture industry is seriously affected by pathogen infections. Of these pathogens, Bombyx mori nucleopolyhedrovirus (BmNPV) causes approximately 80% of the total economic losses due to pathogen infections. We previously constructed a BmNPV-specific CRISPR/Cas9 silkworm line with significantly enhanced resistance to BmNPV. In order to optimize the resistance properties and minimize its impact on economic traits, we constructed an inducible CRISPR/Cas9 system for use in transgenic silkworms. We used the 39k promoter, which is induced by viral infection, to express Cas9 and the U6 promoter to express four small guide RNA targeting the genes encoding BmNPV late expression factors 1 and 3 (lef-1 and lef-3, respectively), which are essential for viral DNA replication. The system was rapidly activated when the silkworm was infected and showed considerably higher resistance to BmNPV infection than the wild-type silkworm. The inducible system significantly reduced the development effects due to the constitutive expression of Cas9. No obvious differences in developmental processes or economically important characteristics were observed between the resulting transgenic silkworms and wild-type silkworms. Adoption of this accurate and highly efficient inducible CRISPR/Cas9 system targeting BmNPV DNA replication will result in enhanced antivirus measures during sericulture, and our work also provides insights into the broader application of the CRISPR/Cas9 system in the control of infectious diseases and insect pests.
Inhibitory Effect of IL-1β on HBV and HDV Replication and HBs Antigen-Dependent Modulation of Its Secretion by Macrophages
Authors: Delphin Marion , Faure-Dupuy Suzanne , Isorce Nathalie , Rivoire Michel , Salvetti Anna , Durantel David , Lucifora Julie
Published: December 2021

Co-infection with the hepatitis B virus and hepatitis delta virus (HDV) leads to the most aggressive form of viral hepatitis. Using in vitro infection models, we confirmed that IL-1β, a crucial innate immune molecule for pathogen control, was very potent against HBV from different genotypes. Additionally, we demonstrated for the first time a strong and rapid antiviral effect induced by very low doses of IL-1β against HDV. In parallel, using co-culture assays, we demonstrated that monocytes exposed to HBV, and in particular to HBsAg, during differentiation into pro-inflammatory macrophages secreted less IL-1β. Altogether, our data emphasize the importance of developing combined antiviral strategies that would, for instance, reduce the secretion of HBsAg and stimulate the immune system to produce endogenous IL-1β efficient against both HBV and HDV.
Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece
Authors: Fontana Stefano , Buttinelli Gabriele , Fiore Stefano , Amato Concetta , Pataracchia Marco , Kota Majlinda , Aćimović Jela , Blažević Mia , Mulaomerović Mirsada , Nikolaeva-Glomb Lubomira , Mentis Andreas , Voulgari-Kokota Androniki , Gashi Luljeta , Kaçaniku-Gunga Pranvera , Barbara Christopher , Melillo Jackie , Protic Jelena , Filipović-Vignjevic Svetlana , O’Connor Patrick M. , D’Alberto Alessandra , Orioli Riccardo , Siddu Andrea , Saxentoff Eugene , Stefanelli Paola
Published: December 2021

Here we analyzed six years of acute flaccid paralysis (AFP) surveillance, from 2015 to 2020, of 10 countries linked to the WHO Regional Reference Laboratory, at the Istituto Superiore di Sanità, Italy. The analysis also comprises the polio vaccine coverage available (2015–2019) and enterovirus (EV) identification and typing data. Centralized Information System for Infectious Diseases and Laboratory Data Management System databases were used to obtain data on AFP indicators and laboratory performance and countries’ vaccine coverage from 2015 to 2019. EV isolation, identification, and typing were performed by each country according to WHO protocols. Overall, a general AFP underreporting was observed. Non-Polio Enterovirus (NPEV) typing showed a high heterogeneity: over the years, several genotypes of coxsackievirus and echovirus have been identified. The polio vaccine coverage, for the data available, differs among countries. This evaluation allows for the collection, for the first time, of data from the countries of the Balkan area regarding AFP surveillance and polio vaccine coverage. The need, for some countries, to enhance the surveillance systems and to promote the polio vaccine uptake, in order to maintain the polio-free status, is evident.
Immunogenicity of a Candidate DTacP-sIPV Combined Vaccine and Its Protection Efficacy against Pertussis in a Rhesus Macaque Model
Authors: Wang Xiaoyu , Gao Na , Wen Jiana , Li Jingyan , Ma Yan , Sun Mingbo , Liang Jiangli , Shi Li
Published: December 2021

The research and development of a pertussis-combined vaccine using a novel inactivated poliovirus vaccine made from the Sabin strain (sIPV) is of great significance in the polio eradication project and to address the recent resurge in pertussis. In the present study, we compared the immunogenicity and efficacy of a candidate DTacP-sIPV with those of a commercial DTacP-wIPV/Hib, DTaP/Hib, pertussis vaccine, and aluminum hydroxide adjuvant control in the rhesus macaque model with a 0-, 1-, and 2-month immunization schedule. At day 28 after the third dose, rhesus macaques were challenged with aerosol pertussis and the antibody and cellular response together with pertussis clinical symptoms were determined. The production of anti-PT, anti-PRN, anti-FHA, anti-DT, anti-TT, and polio type I, II, III antibodies was induced by the candidate DTacP-sIPV, which was as potent as commercial vaccines. In comparison with the control group that showed typical pertussis symptoms of humans after the aerosol challenge, the DTacP-sIPV group did not exhibit obvious clinical pertussis symptoms and had higher neutralization titers of anti-PT, anti-PRN, and anti-FHA. In conclusion, the DTacP-sIPV vaccine was able to induce immunity in rhesus macaques to prevent pertussis infections after immunization. The developed vaccine was as efficient as other commercial vaccines.
Sociodemographic Predictors Associated with the Willingness to Get Vaccinated against COVID-19 in Peru: A Cross-Sectional Survey
Authors: Vizcardo David , Salvador Linder Figueroa , Nole-Vara Arian , Dávila Karen Pizarro , Alvarez-Risco Aldo , Yáñez Jaime A. , Mejia Christian R.
Published: December 2021

During the race for the development of a vaccine against COVID-19, even before its commercialization, part of the population has already shown a growing fear of its application. We designed an analytical cross-sectional study using an anonymous survey in the 25 departments of Peru. We surveyed whether the participants were planning on getting vaccinated, as well as other characteristics that were cross-checked in a uni-, bi- and multivariate manner. Of the 1776 respondents, 70% (1251) stated that they were planning to be vaccinated, 20% (346) did not know yet or doubted it, and 10% (179) did not want to be vaccinated. We observed that those who did not get infected with COVID-19 exhibited a higher frequency to not wanting or were uncertain about getting vaccinated (aPR: 1.40; 95% CI: 1.09–1.81; p-value = 0.008). In contrast, there was a lower frequency of vaccine refusal among university students (aPR: 0.75; 95% CI: 0.61–0.92; p-value = 0.005) and healthcare workers (aPR: 0.59; 95% CI: 0.44–0.80; p-value = 0.001); adjusted by place of residence. There is still an important percentage of respondents who do not want to be vaccinated or are hesitant to do it, which was associated with educational level, being a healthcare worker and if they were previously infected with COVID-19. Our results could offer useful information about COVID-19 vaccination campaigns.
Evaluating the Geographical Accessibility and Equity of COVID-19 Vaccination Sites in England
Authors: Duffy Catherine , Newing Andy , Górska Joanna
Published: December 2021

We assess the geographical accessibility of COVID-19 vaccination sites—including mass vaccination centers and community-level provision—in England utilizing open data from NHS England and detailed routing data from HERE Technologies. We aim to uncover inequity in vaccination site accessibility, highlighting small-area inequality hidden by coverage figures released by the NHS. Vaccination site accessibility measures are constructed at a neighborhood level using indicators of journey time by private and public transport. We identify inequity in vaccination-site accessibility at the neighborhood level, driven by region of residence, mode of transport (specifically availability of private transport), rural-urban geography and the availability of GP-led services. We find little evidence that accessibility to COVID-19 vaccination sites is related to underlying area-based deprivation. We highlight the importance of GP-led provision in maintaining access to vaccination services at a local level and reflect on this in the context of phase 3 of the COVID-19 vaccination programme (booster jabs) and other mass vaccination programmes.
Prevalence, Risk Factors, Antimicrobial Resistance and Molecular Characterization of Salmonella in Northeast Tunisia Broiler Flocks
Authors: Oueslati Walid , Rjeibi Mohamed Ridha , Benyedem Hayet , Mamlouk Aymen , Souissi Fatma , Selmi Rachid , Ettriqui Abdelfettah
Published: December 2021

This study was conducted in northeastern Tunisia to estimate both the prevalence and the risk factors of Salmonella in broiler flocks as well as to characterize the isolated multidrug-resistant (MDR) Salmonella strains. In the present study, a total number of 124 farms were sampled; Salmonella isolates were identified by the alternative technique VIDAS Easy Salmonella. The susceptibility of Salmonella isolates was assessed against 21 antimicrobials using the disk diffusion method on Mueller–Hinton agar using antimicrobial discs. Some antimicrobial resistance genes were identified using PCR. The prevalence rate of Salmonella infection, in the sampled farms, was estimated at 19.9% (64/322). Moreover, a total number of 13 different serotypes were identified. High rate of resistance was identified against nalidixic acid (82.85%), amoxicillin (81.25%), streptomycin (75%), and ciprofloxacin (75%). Alarming level of resistance to ertapenem (12.5%) was noticed. A total of 87.5% (56/64) of isolated strains were recognized as MDR. Three MDR strains were extended-spectrum β-lactamases (ESBL)-producers and three MDR strains were cephalosporinase-producers. The blaCTX-M gene was amplified in all the three ESBL strains. The qnrB gene was not amplified in fluoroquinolones-resistant strains. The tetA and tetB genes were amplified in 5% (2/40) and 2.5% (1/40) of tetracycline-resistant strains, respectively. The dfrA1 gene was amplified in five of the 20 trimethoprim-resistant strains. The mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 genes were not amplified in any of the phenotypically colistin-resistant strains. In terms of integrase genes int1 and int2, only gene class 2 was amplified in 11% (7/64) of analyzed strains. Risk factors, such as the poor level of cleaning and disinfection, the lack of antimicrobial treatment at the start of the breeding, and a crawl space duration lower than 15 days, were associated with high Salmonella infection in birds. These data should be considered when preparing salmonellosis control programs in Tunisian broiler flocks.
Diversity of Silica-Scaled Chrysophytes in Central Vietnam
Authors: Gusev Evgeniy , Martynenko Nikita
Published: December 2021

This paper focuses on the flora of scale-bearing chrysophytes from eight provinces located in the central part of Vietnam. Khanh Hoa, Phu Yen, Binh Dinh, Thua Thien Hue, Quang Tri, and Quang Binh provinces are located in the coastal area of Vietnam. Lam Dong and Dak Lak provinces represent mountain territories with an elevation of 500–2000 metres above sea level. In total, 212 water bodies of different origins were studied. Samples were obtained from swamp areas, lakes, rivers, reservoirs, ponds, and small temporary water bodies. In total, 76 taxa were identified by electron microscopic observations of samples. A total of 54 taxa were found in the mountainous provinces, while 73 were found in the coastal provinces. Of these, 51 species are common for both areas. The most diverse was the genus Mallomonas with 66 species, varieties, and forms; followed by Synura with 7 taxa; Chrysosphaerella with 2; and Spiniferomonas with 1. Seven taxa of the genus Mallomonas were not identified to the lower rank. All these unidentified specimens may potentially represent new species for science. Ten taxa are reported for the first time in Vietnam.
British hybrids involving Epilobium tetragonum subsp tournefortii (Onagraceae), Tournefort’s Willowherb
Authors: Kitchener Geoffrey , Leslie Alan , Leonard Peter
Published: December 2021

A study of populations of Tournefort’s Willowherb, Epilobium tetragonum L. subsp. tournefortii (Michalet) Lév., a non-native taxon naturalised in Cambridgeshire, revealed the presence of plants which on the basis of their morphology were attributed to hybrids with native willowherbs Epilobium parviflorum Schreb. (Hoary Willowherb), Epilobium tetragonum L. subsp. tetragonum (Square-stalked Willowherb) and Epilobium hirsutum L. (Great Willowherb). Details of distinguishing characters are given. The hybrid taxa have not previously been recorded as such in the British Isles.