bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship of Graduate Students with their Academic Supervisors: A Study of Public and Private Sector Universities of Pakistan
Authors: Sohail Dania , Alam Malik Shahab
Published: July 2021

This study is aimed to observe different aspects of working relationship between supervisees with their academic supervisors from the supervisees’ perspective. Respondents were inquired about networking, instrumental, and psychosocial help received from their supervisors; their satisfaction level with their supervisors; and about their behavioural intentions. Supervisees’ satisfaction was used as a mediator here. A descriptive study was carried out in public and private sector universities. Respondents were 350 MS and PhD level students. Linear Regression was used to analyze relationships among variables. Full mediation was observed in private sector universities, whereas partial mediation was found in public sector universities. Independent sample t-test was employed to observe significant mean differences between public and private sector universities. Significant mean scores differences were observed in independent samples t-test in instrumental and psychosocial help between these two types of universities.
Contemporary dance as being and becoming in the age of aging
Authors: Ferm Almqvist Cecilia
Published: July 2021

This article takes experiences from a contemporary multi-artistic dance project as a starting-point. The aim is to describe and explore how such a project can offer possibilities for being and becoming among elderly amateur dancers, based on a phenomenological way of thinking. The phenomenon of the investigation is self-conceptualization. The multi-artistic process and context is defined as an adult educational situation. To come close to the lived experiences of the dancers, the rehearsals as well as the performance were observed, and documented. Six of the participants were also interviewed. The material was analyzed in a hermeneutical phenomenological manner, and de Beauvoir’s thinking regarding aging, was used as a theoretical lens. The results show how the self-images of the participants change throughout the project. The dance activities seem to give the elderly possibilities to remain themselves, even if they become different. They learn to know themselves, each other and the world.
The Synaptic Vesicle Protein 2A Interacts With Key Pathogenic Factors in Alzheimer’s Disease: Implications for Treatment
Authors: Kong Yanyan , Huang Lin , Li Weihao , Liu Xuanting , Zhou Yinping , Liu Cuiping , Zhang Shibo , Xie Fang , Zhang Zhengwei , Jiang Donglang , Zhou Weiyan , Ni Ruiqing , Zhang Chencheng , Sun Bomin , Wang Jiao , Guan Yihui
Published: July 2021

Alzheimer’s disease (AD), a serious neurodegenerative disease, is pathologically characterized by synaptic loss and dysfunction. Synaptic vesicle protein 2A (SV2A) is an indispensable vesicular protein specifically expressed in synapses and can be used as a biomarker for synaptic density. We found that the expression of SV2A was down-regulated in the hippocampus of AD patients, yet the relation of SV2A to other hallmarks of AD pathology such as amyloid precursor protein (APP), β-amyloid (Aβ), and Tau protein is not thoroughly clear. In addition, SV2A colocalized with APP and was down-regulated at Aβ deposition. Moreover, we found that SV2A deficiency leads to a simultaneous increase in Aβ and Tau hyperphosphorylation, while SV2A overexpression was associated with downregulation of β-site APP cleaving enzyme 1 and apolipoprotein E genes. In addition, evidence gained in the study points to the phosphatidylinositol 3-kinase signaling pathway as a possible mediator in SV2A regulation influencing the incidence and development of AD. With limited effective diagnostic methods for AD, a close interplay between SV2A and AD-related proteins demonstrated in our study may provide novel and innovative diagnostic and therapeutic opportunities.
NTNG1 Modulates Cisplatin Resistance in Epithelial Ovarian Cancer Cells via the GAS6/AXL/Akt Pathway
Authors: Fang Shanyu , Luo Yuanyuan , Zhang Ying , Wang Houmei , Liu Qianfen , Li Xinya , Yu Tinghe
Published: July 2021

Cisplatin resistance is a challenge in the treatment of epithelial ovarian cancer. Here, clinical data showed that the level of netrin-G1 (NTNG1) in cisplatin-resistant cancer was higher than that in cisplatin-sensitive cancer (2.2-fold, p = 0.005); patients with a high NTNG1 level in cancer tissues had shorter progression-free survival (11.0 vs. 25.0 months, p = 0.010) and platinum-free interval (5.0 vs. 20.0 months, p = 0.021) compared with patients with a low level. Category- or stage-adjusted analyses demonstrated that the association between the NTNG1 level and prognosis occurred in type II or FIGO III/IV cancer. The basal level of NTNG1 in SKOV3/DDP cells (a cisplatin-resistant subline) was higher than that in SKOV3 cells; therefore, NTNG1 was overexpressed in SKOV3 cells, or silenced in SKOV3/DDP cells. Knocking in NTNG1 reduced the action of cisplatin to decrease cell death and apoptosis of SKOV3 cells, accompanied by upregulation of p-AXL, p-Akt and RAD51; however, opposite effects were observed in SKOV3/DDP cells after knocking down NTNG1. Co-immunoprecipitation demonstrated that NTNG1 bound GAS6/AXL. Silencing NTNG1 enhanced cisplatin effects in vivo, decreasing tumor volume/mass. These data suggested that a high NTNG1 level can result in cisplatin resistance in ovarian cancer cells via the GAS6/AXL/Akt pathway and that NTNG1 may be a useful target to overcome resistance.
Distinct Gene Expression Patterns of Two Heat Shock Protein 70 Members During Development, Diapause, and Temperature Stress in the Freshwater Crustacean Daphnia magna
Authors: Chen Luxi , Gómez Rocío , Weiss Linda C.
Published: July 2021

Dormancy is a lifecycle delay that allows organisms to escape suboptimal environmental conditions. As a genetically programmed type of dormancy, diapause is usually accompanied by metabolic depression and enhanced tolerance toward adverse environmental factors. However, the drivers and regulators that steer an organism’s development into a state of suspended animation to survive environmental stress have not been fully uncovered. Heat shock proteins 70 (HSP70s), which are often produced in response to various types of stress, have been suggested to play a role in diapause. Considering the diversity of the Hsp70 family, different family members may have different functions during diapause. In the present study, we demonstrate the expression of two hsp70 genes (A and B together with protein localization of B) throughout continuous and diapause interrupted development of Daphnia magna. Before and after diapause, the expression of Dmhsp70-A is low. Only shortly before diapause and during diapause, Dmhsp70-A is significantly upregulated and may therefore be involved in diapause preparation and maintenance. In contrast, Dmhsp70-B is expressed only in developing embryos but not in diapausing embryos. During continuous development, the protein of this Hsp70 family member is localized in the cytosol. When we expose both embryo types to heat stress, expression of both hsp70 genes increases only in developing embryos, and the protein of family member B is translocated to the nucleus. In this stress formation, this protein provides effective protection of nucleoplasmic DNA. As we also see this localization in diapausing embryos, it seems that Daphnia embryo types share a common subcellular strategy when facing dormancy or heat shock, i.e., they protect their DNA by HSP70B nuclear translocation. Our study underlines the distinctive roles that different Hsp70 family members play throughout continuous and diapause interrupted development.
Synthesis and Structure of Oxygen Deficient Lead-Technetium Pyrochlore, the First Example of a Valence V Technetium Oxide
Authors: Kennedy Brendan J. , Ablott Timothy A. , Avdeev Maxim , Carter Melody L. , Losurdo Linda , Saura-Muzquiz Matilde , Thorogood Kevin J. , Ting Jimmy , Wallwork Kia S. , Zhang Zhaoming , Zhu Hanliang , Thorogood Gordon J.
Published: July 2021

The structure of lead-technetium pyrochlore has been refined in space group Fd3¯m with a = 10.36584(2) Å using a combination of synchrotron X-ray and neutron powder diffraction data and confirmed via Electron Diffraction. The oxide is found to be oxygen deficient with a stoichiometry of Pb2Tc2O7-d. Displacive disorder of the Pb cations is evident from the refinements, as has been observed in Bi2Tc2O7-d. X-ray absorption spectroscopic measurements at the Tc K-edge demonstrate the valence of the Tc is greater than 4.0 as anticipated from the refined oxygen stoichiometry. Raman spectroscopy confirms the presence of disorder leading us to conclude that this pyrochlore is the first example of a valence V technetium oxide.
Moral Expressions in 280 Characters or Less: An Analysis of Politician Tweets Following the 2016 Brexit Referendum Vote
Authors: van Vliet Livia
Published: July 2021

Ideas about morality are deeply entrenched into political opinions. This article examines the online communication of British parliamentarians from May 2017-December 2019, following the 2016 referendum that resulted in Britain's exit (Brexit) from the European Union. It aims to uncover how British parliamentarians use moral foundations to discuss the Brexit withdrawal agreement on Twitter, using Moral Foundations Theory as a classification basis for their tweets. It is found that the majority of Brexit related tweets contain elements of moral reasoning, especially relating to the foundations of Authority and Loyalty. There are common underlying foundations between parties, but parties express opposing viewpoints within a single foundation. The study provides useful insights into Twitter’s use as an arena for moral argumentation, as well as uncovers the politician’s uses of moral arguments during Brexit agreement negotiations on Twitter. It contributes to the limited body of work focusing on the moral arguments made by politicians through Twitter.
Local and Remote Atmospheric Circulation Drivers of Arctic Change: A Review
Authors: Henderson Gina R. , Barrett Bradford S. , Wachowicz Lori J. , Mattingly Kyle S. , Preece Jonathon R. , Mote Thomas L.
Published: July 2021

Arctic Amplification is a fundamental feature of past, present, and modelled future climate. However, the causes of this “amplification” within Earth’s climate system are not fully understood. To date, warming in the Arctic has been most pronounced in autumn and winter seasons, with this trend predicted to continue based on model projections of future climate. Nevertheless, the mechanisms by which this will take place are numerous, interconnected. and complex. Will future Arctic Amplification be primarily driven by local, within-Arctic processes, or will external forces play a greater role in contributing to changing climate in this region? Motivated by this uncertainty in future Arctic climate, this review seeks to evaluate several of the key atmospheric circulation processes important to the ongoing discussion of Arctic amplification, focusing primarily on processes in the troposphere. Both local and remote drivers of Arctic amplification are considered, with specific focus given to high-latitude atmospheric blocking, poleward moisture transport, and tropical-high latitude subseasonal teleconnections. Impacts of circulation variability and moisture transport on sea ice, ice sheet surface mass balance, snow cover, and other surface cryospheric variables are reviewed and discussed. The future evolution of Arctic amplification is discussed in terms of projected future trends in atmospheric blocking and moisture transport and their coupling with the cryosphere. As high-latitude atmospheric circulation is strongly influenced by lower-latitude processes, the future state of tropical-to-Arctic teleconnections is also considered.
Lagomorpha as a Model Morphological System
Authors: Kraatz Brian , Belabbas Rafik , Fostowicz-Frelik Łucja , Ge De-Yan , Kuznetsov Alexander N. , Lang Madlen M. , López-Torres Sergi , Mohammadi Zeinolabedin , Racicot Rachel A. , Ravosa Matthew J. , Sharp Alana C. , Sherratt Emma , Silcox Mary T. , Słowiak Justyna , Winkler Alisa J. , Ruf Irina
Published: July 2021

Due to their global distribution, invasive history, and unique characteristics, European rabbits are recognizable almost anywhere on our planet. Although they are members of a much larger group of living and extinct mammals [Mammalia, Lagomorpha (rabbits, hares, and pikas)], the group is often characterized by several well-known genera (e.g.,Oryctolagus,Sylvilagus,Lepus, andOchotona). This representation does not capture the extraordinary diversity of behavior and form found throughout the order. Model organisms are commonly used as exemplars for biological research, but there are a limited number of model clades or lineages that have been used to study evolutionary morphology in a more explicitly comparative way. We present this review paper to show that lagomorphs are a strong system in which to study macro- and micro-scale patterns of morphological change within a clade that offers underappreciated levels of diversity. To this end, we offer a summary of the status of relevant aspects of lagomorph biology.
Novel 61-bp Indel of RIN2 Is Associated With Fat and Hatching Weight Traits in Chickens
Authors: Lin Wujian , Ren Tuanhui , Li Wangyu , Liu Manqing , He Danlin , Liang Shaodong , Luo Wen , Zhang Xiquan
Published: July 2021

The Ras and Rab interactor 2 (RIN2) gene, which encodes RAS and Rab interacting protein 2, can interact with GTP-bound Rab5 and participate in early endocytosis. This study found a 61-bp insertion/deletion (indel) in the RIN2 intron region, and 3 genotypes II, ID, and DD were observed. Genotype analysis of mutation sites was performed on 665 individuals from F2 population and 8 chicken breeds. It was found that the indel existed in each breed and that yellow feathered chickens were mainly of the DD genotype. Correlation analysis of growth and carcass traits in the F2 population of Xinghua and White Recessive Rock chickens showed that the 61-bp indel was significantly correlated with abdominal fat weight, abdominal fat rate, fat width, and hatching weight (P < 0.05). RIN2 mRNA was expressed in all the tested tissues, and its expression in abdominal fat was higher than that in other tissues. In addition, the expression of the RIN2 mRNA in the abdominal fat of the DD genotype was significantly higher than that of the II genotype (P < 0.05). The transcriptional activity results showed that the luciferase activity of the pGL3-DD vector was significantly higher than that of the pGL3-II vector (P < 0.01). Moreover, the results indicate that the polymorphisms in transcription factor binding sites (TFBSs) of 61-bp indel may affect the transcriptional activity of RIN2, and thus alter fat traits in chicken. The results of this study showed that the 61-bp indel was closely related to abdominal fat-related and hatching weight traits of chickens, which may have reference value for molecular marker-assisted selection of chickens.
Distinct Microbiomes of Gut and Saliva in Patients With Systemic Lupus Erythematous and Clinical Associations
Authors: Liu Fengping , Ren Tianli , Li Xiaodi , Zhai Qixiao , Xu Xifeng , Zhang Nan , Jiang Peng , Niu Yaofang , Lv Longxian , Shi GuoXun , Feng Ninghan
Published: July 2021

Alterations in the microbiome of the gut and oral cavity are involved in the etiopathogenesis of systemic lupus erythematosus (SLE). We aimed to assess whether both microbiome compositions in feces and saliva were specific in patients with SLE. A total of 35 patients with SLE, as well as sex- and age-matched asymptomatic subjects as healthy control (HC) group were recruited. Fecal swabs and saliva samples were collected from the participants. 16S ribosomal RNA gene sequencing was performed on the samples. Compared with the HC group, reduced bacterial richness and diversity were detected in the feces of patients with SLE, and increased bacterial diversity in their saliva. Both feces and saliva samples explained the cohort variation. The feces were characterized by enrichment of Lactobacillus, and depletion of an unclassified bacterium in the Ruminococcaceae family and Bifidobacterium. Lack of Bifidobacterium was observed in patients with arthritis. Akkermansia and Ruminococcus negatively correlated with the serum levels of C3. In saliva, Veillonella, Streptococcus, and Prevotella were dominant, and Bacteroides was negatively associated with disease activity. These findings can assist us to comprehensively understand the bacterial profiles of different body niches in SLE patients.
STING Agonist Combined to a Protein-Based Cancer Vaccine Potentiates Peripheral and Intra-Tumoral T Cell Immunity
Authors: Rossi Matteo , Carboni Susanna , Di Berardino-Besson Wilma , Riva Erika , Santiago-Raber Marie-Laure , Belnoue Elodie , Derouazi Madiha
Published: July 2021

Combining different immunotherapy approaches is currently building the future of immunotherapy, with the view to maximize anti-tumoral efficacy for larger patient population. The KISIMA™ platform allows the development of protein-based cancer vaccines able to induce tumor-specific T cell response resulting in anti-tumoral efficacy in various mouse models. Intra-tumoral administration of stimulator of interferon gene agonists (STINGa) was shown to induce a potent inflammatory response leading to the development of tumor-specific immunity. Here, we explored the efficacy and mechanisms of action of subcutaneous STINGa treatment combined with therapeutic vaccination in various mouse tumor models. This combinatory treatment highly enhanced frequency and effector function of both peripheral and intra-tumoral antigen-specific CD8 T cells, promoting potent IFNγ and TNFα production along with increased cytotoxicity. Moreover, combination therapy favorably modulated the tumor microenvironment by dampening immune-suppressive cells and increasing CD4 T cell infiltration together with their polarization toward Th1 phenotype. Combination with STINGa treatment improved the effect of therapeutic vaccination, resulting in a prolonged control and slower growth of B16-OVA and TC-1 tumors. Altogether, the results presented here highlight the potential of combining STINGa with a therapeutic protein vaccine for cancer treatment.
Microglia, Cytokines, and Neural Activity: Unexpected Interactions in Brain Development and Function
Authors: Ferro Austin , Auguste Yohan S. S. , Cheadle Lucas
Published: July 2021

Intercellular signaling molecules such as cytokines and their receptors enable immune cells to communicate with one another and their surrounding microenvironments. Emerging evidence suggests that the same signaling pathways that regulate inflammatory responses to injury and disease outside of the brain also play powerful roles in brain development, plasticity, and function. These observations raise the question of how the same signaling molecules can play such distinct roles in peripheral tissues compared to the central nervous system, a system previously thought to be largely protected from inflammatory signaling. Here, we review evidence that the specialized roles of immune signaling molecules such as cytokines in the brain are to a large extent shaped by neural activity, a key feature of the brain that reflects active communication between neurons at synapses. We discuss the known mechanisms through which microglia, the resident immune cells of the brain, respond to increases and decreases in activity by engaging classical inflammatory signaling cascades to assemble, remodel, and eliminate synapses across the lifespan. We integrate evidence from (1) in vivo imaging studies of microglia-neuron interactions, (2) developmental studies across multiple neural circuits, and (3) molecular studies of activity-dependent gene expression in microglia and neurons to highlight the specific roles of activity in defining immune pathway function in the brain. Given that the repurposing of signaling pathways across different tissues may be an important evolutionary strategy to overcome the limited size of the genome, understanding how cytokine function is established and maintained in the brain could lead to key insights into neurological health and disease.
Targeting Mitochondrial-Derived Reactive Oxygen Species in T Cell-Mediated Autoimmune Diseases
Authors: Chávez Miranda D. , Tse Hubert M.
Published: July 2021

Mitochondrial dysfunction resulting in oxidative stress could be associated with tissue and cell damage common in many T cell-mediated autoimmune diseases. Autoreactive CD4 T cell effector subsets (Th1,Th17) driving these diseases require increased glycolytic metabolism to upregulate key transcription factors (TF) like T-bet and RORγt that drive differentiation and proinflammatory responses. However, research in immunometabolism has demonstrated that mitochondrial-derived reactive oxygen species (ROS) act as signaling molecules contributing to T cell fate and function. Eliminating autoreactive T cells by targeting glycolysis or ROS production is a potential strategy to inhibit autoreactive T cell activation without compromising systemic immune function. Additionally, increasing self-tolerance by promoting functional immunosuppressive CD4 T regulatory (Treg) cells is another alternative therapeutic for autoimmune disease. Tregs require increased ROS and oxidative phosphorylation (OxPhos) for Foxp3 TF expression, differentiation, and anti-inflammatory IL-10 cytokine synthesis. Decreasing glycolytic activity or increasing glutathione and superoxide dismutase antioxidant activity can also be beneficial in inhibiting cytotoxic CD8 T cell effector responses. Current treatment options for T cell-mediated autoimmune diseases such as Type 1 diabetes (T1D), multiple sclerosis (MS), rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) include global immunosuppression, antibodies to deplete immune cells, and anti-cytokine therapy. While effective in diminishing autoreactive T cells, they can also compromise other immune responses resulting in increased susceptibility to other diseases and complications. The impact of mitochondrial-derived ROS and immunometabolism reprogramming in autoreactive T cell differentiation could be a potential target for T cell-mediated autoimmune diseases. Exploiting these pathways may delay autoimmune responses in T1D.
Thymic Egress Is Regulated by T Cell-Derived LTβR Signal and via Distinct Thymic Portal Endothelial Cells
Authors: Xia Huan , Zhong Suijuan , Zhao Yixiao , Ren Boyang , Wang Zhongnan , Shi Yaoyao , Chai Qian , Wang Xiaoqun , Zhu Mingzhao
Published: July 2021

Thymic blood vessels at the perivascular space (PVS) are the critical site for both homing of hematopoietic progenitor cells (HPCs) and egress of mature thymocytes. It has been intriguing how different opposite migrations can happen in the same place. A subset of specialized thymic portal endothelial cells (TPECs) associated with PVS has been identified to function as the entry site for HPCs. However, the cellular basis and mechanism underlying egress of mature thymocytes has not been well defined. In this study, using various conventional and conditional gene-deficient mouse models, we first confirmed the role of endothelial lymphotoxin beta receptor (LTβR) for thymic egress and ruled out the role of LTβR from epithelial cells or dendritic cells. In addition, we found that T cell-derived ligands lymphotoxin (LT) and LIGHT are required for thymic egress, suggesting a crosstalk between T cells and endothelial cells (ECs) for thymic egress control. Furthermore, immunofluorescence staining analysis interestingly showed that TPECs are also the exit site for mature thymocytes. Single-cell transcriptomic analysis of thymic endothelial cells suggested that TPECs are heterogeneous and can be further divided into two subsets depending on BST-1 expression level. Importantly, BST-1hi population is associated with thymic egressing thymocytes while BST-1lo/− population is associated with HPC settling. Thus, we have defined a LT/LIGHT-LTβR signaling–mediated cellular crosstalk regulating thymic egress and uncovered distinct subsets of TPECs controlling thymic homing and egress, respectively.
Genetic Structure of the Goniopora lobata and G. djiboutiensis Species Complex Is Better Explained by Oceanography Than by Morphological Characteristics
Authors: Yasuda Nina , Kitano Yuko F. , Taninaka Hiroki , Nagai Satoshi , Mezaki Takuma , Yamashita Hiroshi
Published: July 2021

Species delimitation of closely related corals is often challenging due to high intraspecies morphological variation and phenotypic plasticity with a lack of characteristic features and scarcity of relevant molecular markers. Goniopora spp. are one such coralline group, and the species status of Goniopora lobata and Goniopora djiboutiensis, an Indian and Pacific Ocean hermatypic coral species complex, has been questioned on the basis of previous molecular and morphological analyses. To further examine the species boundaries between G. lobata and G. djiboutiensis in Japan, specimens collected from areas spanning from Ryukyu Island to temperate Japanese regions were morphologically identified based on traditional morphological descriptions. Then, the genetic structure of the G. lobata and G. djiboutiensis species complex was examined using six newly developed microsatellite markers. The majority of the collected specimens had intermediate morphologies, and a STRUCTURE analysis using the LOCPRIOR model based on typical G. lobata and G. djiboutiensis morphology indicated that there were no genetic differences between these morphologies. On the other hand, STRUCTURE analysis based on oceanographic regions revealed that there was a genetic break between the temperate and subtropical regions. This weak genetic break corresponded with the Kuroshio-associated barrier, which prevents larval transport between subtropical and temperate regions. This study confirms that the current morphological identification criteria for G. lobata and G. djiboutiensis do not match the existing genetic boundaries and thus the two should be regarded as a species complex. This study also highlighted the robustness of using a multi-locus population genetic approach, including a geographic context, to confirm the species boundaries of closely related species.
Megafaunal Ecology of the Western Clarion Clipperton Zone
Authors: Durden Jennifer M. , Putts Meagan , Bingo Sarah , Leitner Astrid B. , Drazen Jeffrey C. , Gooday Andrew J. , Jones Daniel O. B. , Sweetman Andrew K. , Washburn Travis W. , Smith Craig R.
Published: July 2021

The Clarion Clipperton Zone (CCZ) is a vast area of the central Pacific Ocean where the abyssal seabed is a focus for future polymetallic nodule mining. Broad-scale environmental gradients occur east-to-west across the CCZ seabed, including organic matter supply and nodule abundance, factors that influence benthic faunal community structure and function. A network of protected areas across the CCZ, called Areas of Particular Environmental Interest (APEIs), has been designated to cover this variation. Most previous studies of the benthic environment and megafaunal communities have focussed on the eastern CCZ, leaving the impact of these large-scale gradients unexamined and the network design untested. Seamounts are a further source of heterogeneity in the region. We examined the benthic megafaunal ecology of three APEIs in the western CCZ, spanning a range of environmental conditions. We used a combination of seabed photography and direct sampling to assess the environment and megafauna on the soft sediment habitats on the abyssal plain in three APEIs, and seamounts in two of those APEIs. We found that environmental conditions on abyssal plains differed between the three APEIs in terms of water depth, nodule abundance and coverage, sediment particle size distribution, and estimated organic matter flux. Megafauna were low density and high diversity, with few common morphotypes between sites and many morphotypes being observed only once. Xenophyophores dominated the assemblages. The density and diversity of invertebrates were greater at the sites with lower organic matter inputs and greater nodule abundance. Seamounts in the same APEIs were nodule-free and had coarser sediments than on the plain. Invertebrate megafaunal diversity was lower on the seamounts than on the plains, and most morphotypes recorded on the seamounts were only found on seamounts. Low morphotype overlap also suggests little connectivity between APEIs, and between seamounts and adjacent abyssal plains. Our results provide the first evaluation of the seabed habitats and megafaunal ecology in the western CCZ, highlighting environmental gradients that influence benthic communities, and are important for evaluating the design of the network of protected areas.
Reshaping of Gut Microbiota in Gilthead Sea Bream Fed Microbial and Processed Animal Proteins as the Main Dietary Protein Source
Authors: Solé-Jiménez Paula , Naya-Català Fernando , Piazzon M. Carla , Estensoro Itziar , Calduch-Giner Josep À. , Sitjà-Bobadilla Ariadna , Van Mullem Danny , Pérez-Sánchez Jaume
Published: July 2021

The present study aimed to unravel the effects of partial (50%; 50LSAqua) and total (100%; 100LSAqua) replacement of fish meal (FM) by a commercial protein source (LSAqua SusPro) made of bacterial and processed animal proteins (PAP) in farmed juveniles of gilthead sea bream (Sparus aurata). The trial lasted 8 weeks, and the feasibility of replacement was assessed in terms of growth performance, histopathological scoring and composition of mucosal adherent microbiota from anterior intestine (AI). Specific growth rates (SGR) of 50LSAqua fish were undistinguishable from the CTRL group, whereas a slight but significant growth impairment was found with the total replacement. Histological signs of inflammation across the intestine were more evident at the highest level of FM replacement, and the total concentration of short chain fatty acids (SCFA) in stripped feces decreased in a dose dependent manner. Illumina sequencing of gut mucosal microbiota yielded a mean of 130,439 reads per sample assigned to 1,567 OTUs at 97% identity threshold. The bacterial richness was similar in all groups, but a significantly higher Simpson diversity index was found in 100LSAqua fish. At the phylum level, Proteobacteria were the most abundant in all groups, whereas Firmicutes decreased and Actinobacteria increased with the FM replacement. At a closer look, pro-inflammatory Gammaproteobacteria of the genus Psychrobacter, and Acinetobacter decreased with FM replacement, whereas the anti-inflammatory Paracoccus, Arthrobacter, and Actinomycetales increased, with a remarkable presence of the Propioniciclava genus in LSAqua groups. The inferred metagenome analysis suggested that these discriminant bacteria could be implicated in a counter-regulatory anti-inflammatory response. It also pointed to an over-representation of mucosal microbes that can potentially be involved in the natural production of antimicrobials in fish fed the experimental diets.
Effect of Welding Heat Input on the Microstructure and Impact Toughness of HAZ in 420 MPa-Grade Offshore Engineering Steel
Authors: Liu Dekun , Yang Jian , Zhang Yinhui , Qiu Yunlong , Cheng Guoguang , Yao Meiyi , Dong Jianxin
Published: July 2021

In the present work, the effect of the welding heat input on the microstructure, martensite–austenite (M–A) constituents, and impact toughness of the coarse-grained heat-affected zone (CGHAZ) in offshore engineering steel with Ca deoxidation is studied. With the heat input increased from 50 to 100 kJ/cm, the HAZ toughness decreased rapidly, while the measured microhardness decreases steadily. The grain sizes are increased from 52 to 132 μm, and the width of bainite lath increased from 0.4 to 2 μm. The area fraction of lath bainite (LB) decreased, while the area fraction of granular bainite (GB) increased. The average width of M–A constituents grows from 0.3 to 0.6 μm, and the average length grows from from 0.5 to 0.9 μm. Its area fraction is increased from 5.3 to 8.6% and then decreased to 6.1%, and its number density decreased from 0.7 to 0.2 μm−2. The morphologies of M–A constituents change from dot-like to slender and blocky, which are deleterious to impact toughness. The fracture mechanism changes from ductile to quasicleavage and cleavage as the heat input is increased. As the M–A constituents are always found as the cleavage initiation, they should be responsible for the decrease in HAZ toughness when the heat input is above 100 kJ/cm.
Computational Design of Sensitized Combustion Chemistry Experiments
Authors: Ising Cody , Rodriguez Pedro , Lopez Daniel , Santner Jeffrey
Published: July 2021

In combustion chemistry experiments, reaction rates are often extracted from complex experiments using detailed models. To aid in this process, experiments are performed such that measurable quantities, such as species concentrations, flame speed, and ignition delay, are sensitive to reaction rates of interest. In this work, a systematic method for determining such sensitized experimental conditions is demonstrated. An open-source python script was created using the Cantera module to simulate thousands of 0D and hundreds of 1D combustion chemistry experiments in parallel across a broad, user-defined range of mixture conditions. The results of the simulation are post-processed to normalize and compare sensitivity values among reactions and across initial conditions for time-varying and steady-state simulations, in order to determine the “most useful” experimental conditions. This software can be utilized by researchers as a fast, user-friendly screening tool to determine the thermodynamic and mixture parameters for an experimental campaign. We demonstrate this software through two case studies comparing results of the 0D script against a shock tube experiment and results of the 1D script against a spherical flame experiment. In the shock tube case study we present mixture conditions compared to those used in the literature to study H + O2 (+M)→HO2(+M). In the flame case study, we present mixture conditions compared to those in the literature to study formyl radical (HCO) decomposition and oxidation reactions. The systematically determined experimental conditions identified in the present work are similar to the conditions chosen in the literature.
Use of Almitrine and Inhaled Nitric Oxide in ARDS Due to COVID-19
Authors: Laghlam Driss , Rahoual Ghilas , Malvy Julien , Estagnasié Philippe , Brusset Alain , Squara Pierre
Published: July 2021

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is manifested by an acute respiratory distress syndrome (ARDS) with intense inflammation and endothelial dysfunction leading to particularly severe hypoxemia. We hypothesized that an impaired hypoxic pulmonary vasoconstriction aggravates hypoxemia. The objective of the study was to test the effect of two pulmonary vasoactive drugs on patient oxygenation.

Methods: Observational, single-center, open-label study in one intensive care unit (ICU) of the Paris area, realized in April 2020. Eligible patients had coronavirus disease 2019 (COVID-19) and moderate to severe ARDS [arterial partial pressure of oxygen/fraction of inspired oxygen (PaO2/FiO2) <200 mmHg] despite conventional protective ventilation. Exclusion criteria included pulmonary artery hypertension defined by a pulmonary artery systolic pressure (PAPs) >45 mmHg. The assessment of oxygenation was based on PaO2/FiO2 at (1) baseline, then after (2) 30 min of inhaled nitric oxide (iNO) 10 ppm alone, then (3) 30 min combination of iNO + almitrine infusion 8 μg/kg/min, then (4) 30 min of almitrine infusion alone.

Results: Among 20 patients requiring mechanical ventilation during the study period, 12 met the inclusion criteria. Baseline PaO2/FiO2 was 146 ± 48 mmHg. When iNO was combined with almitrine, PaO2/FiO2 rose to 255 ± 90 mmHg (+80 ± 49%, p = 0.005), also after almitrine alone: 238 ± 98 mmHg (+67 ± 75%, p = 0.02), but not after iNO alone: 185 ± 73 mmHg (+30 ± 5%, p = 0.49). No adverse events related to almitrine infusion or iNO was observed.

Conclusion: Combining iNO and infused almitrine improved the short-term oxygenation in patients with COVID-19-related ARDS. This combination may be of interest when first-line therapies fail to restore adequate oxygenation. These findings argue for an impaired pulmonary hypoxic vasoconstriction in these patients.
NAFLD or MAFLD: Which Has Closer Association With All-Cause and Cause-Specific Mortality?—Results From NHANES III
Authors: Huang Qi , Zou Xiantong , Wen Xin , Zhou Xianghai , Ji Linong
Published: July 2021

Background: The recent change of terminology from non-alcoholic fatty liver disease (NAFLD) to metabolic dysfunction-associated fatty liver disease (MAFLD) has raised heated discussion. We aim to investigate the association of MAFLD or NAFLD with all-cause and cause-specific mortality to compare the outcomes of the two diagnostic criteria in population-based study.

Methods: We recruited 12,480 participants from the Third National Health and Nutrition Examination Survey (NHANES III) with matched mortality data in 2015. Participants were divided into four groups for survival analysis: without NAFLD or MAFLD, with only NAFLD, only MAFLD. Cox proportional hazard regression was used to estimate multivariable-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) for all-cause and cause-specific mortality. Subgroup analysis were applied in MAFLD patients.

Results: The weighted prevalence of MAFLD and NAFLD was relatively 27.4 and 27.9%. Participants with NAFLD or MAFLD were largely overlapped (weighted Cohen's kappa coefficient 0.76). MAFLD increased the overall risk for total mortality in a greater magnitude than NAFLD [HR 2.07 (95% CI 1.86, 2.29) vs. 1.47 (1.20, 1.79)], However, the difference was non-significant after metabolic parameters were adjusted. Risks for cardiovascular, neoplasm, and diabetes-related mortality were similar between MAFLD and NAFLD. Referring to individuals without both NAFLD and MAFLD, individuals with only NAFLD showed reduced total mortality [HR 0.48 (0.34, 0.68)] and neoplasm mortality [HR 0.46 (0.24, 0.89)] in crude. Nevertheless, individuals with only MAFLD independently increased the risk for total mortality [adjusted HR 1.47 (1.22, 1.77)] and neoplasm mortality [aHR 1.58 (1.09, 2.28)]. The risk for overall mortality in MAFLD was consistent between subgroups except for race-ethnicity and whether secondary to viral hepatitis.

Conclusions: Participants with MAFLD or NAFLD were highly concordant. MAFLD showed greater risk for all-cause mortality and equal risk for cause-specific mortality referring to NAFLD. The new terminology excluded participants with lower mortality risk and included participants with higher risk. Drug development for MAFLD should consider ethnic differences.
Association of the Risk of Primary Sjögren's Syndrome With Fibrocystic Breast Disease: A Nationwide, Population-Based Study
Authors: Chen Hsin-Hua , Chen Hsian-Min , Lin Ching-Heng , Tang Kuo-Tung , Chen Der-Yuan , Wei James Cheng-Chung , Chao Wen-Cheng
Published: July 2021

Objective: Primary Sjögren's syndrome (pSS) is characterized by exocrine glandular inflammation; however, the association between preceding mammary-gland-inflammation-related diseases and newly diagnosed pSS remains unexplored.

Methods: We used the 2003–2013 data retrieved from Taiwan's National Health Insurance Research Database (NHIRD) to conduct the present population-based study. We identified newly diagnosed pSS female patients during the 2001–2013 period, as well as age-matched (1:20) and propensity-score-matched (1:2) non-SS individuals (as controls). We explored the associations between pSS and a history of mastitis and fibrocystic breast disease by determining adjusted odds ratios (aORs) with 95% confidence intervals (CIs) using a conditional logistical regression analysis after controlling for potential confounders.

Results: We identified 9,665 patients with pSS and 193,300 age-matched non-SS controls, as well as 9,155 SS cases and 18,310 propensity-score-matched non-SS controls. We found that fibrocystic breast disease (aOR, 1.75; 95% CI, 1.63–1.88) were independently associated with incident SS, whereas mastitis and childbirth-associated breast infections were not associated with incident SS. We also found positive associations between SS and previously reported SS-associated diseases, including cardiovascular diseases, thyroid diseases, pancreatitis, bronchiectasis, infectious diseases, osteoporosis, and ankylosing spondylitis. In the propensity-score-matched populations, the associations between pSS and fibrocystic breast disease (aOR, 1.74; 95% CI, 1.58–1.91) remained consistent.

Conclusion: The present population-based study revealed a previously unexplored association between pSS and history of fibrocystic breast disease, and the finding highlights the need to survey pSS in patients with mammary-gland-inflammation-associated diseases.
ROS and Oxidative Response Systems in Plants Under Biotic and Abiotic Stresses: Revisiting the Crucial Role of Phosphite Triggered Plants Defense Response
Authors: Mohammadi Mohammad Aqa , Cheng Yan , Aslam Mohammad , Jakada Bello Hassan , Wai Myat Hnin , Ye Kangzhuo , He Xiaoxue , Luo Tiantian , Ye Li , Dong Chunxing , Hu Bin , Priyadarshani S. V. G. N. , Wang-Pruski Gefu , Qin Yuan
Published: July 2021

Phosphite (Phi) is a chemical analog of orthophosphate [HPO43−]. It is a systemic pesticide generally known to control the prevalence of oomycetes and soil-borne diseases such as Phytophthora, Pythium, and Plasmopora species. Phi can also control disease symptoms and the spread of pathogenic bacteria, fungi, and nematodes. Phi plays critical roles as a fungicide, pesticide, fertilizer, or biostimulator. Overall, Phi can alleviate the severity of the disease caused by oomycete, fungi, pathogenic bacteria, and nematodes (leave, stem, fruit, tuber, and root) in various plants (vegetables, fruits, crops, root/tuber crops, ornamental plants, and forests). Advance research in molecular, physiological, and biochemical approaches has approved the key role of Phi in enhancing crop growth, quantity, and quality of several plant species. Phi is chemically similar to orthophosphate, and inside the cells, it is likely to get involved in different features of phosphate metabolism in both plants and pathogens. In plants, a range of physiobiochemical alterations are induced by plant pathogen stress, which causes lowered photosynthesis activities, enzymatic activities, increased accumulation of reactive oxygen species (ROS), and modification in a large group of genes. To date, several attempts have been made to study plant-pathogen interactions with the intent to minimize the loss of crop productivity. Phi’s emerging function as a biostimulant in plants has boost plant yield and tolerance against various stress factors. This review discusses Phi-mediated biostimulant effects against biotic and abiotic stresses.
Structure and Dynamics of the Gut Bacterial Community Across the Developmental Stages of the Coffee Berry Borer, Hypothenemus hampei
Authors: Mejía-Alvarado Fernan Santiago , Ghneim-Herrera Thaura , Góngora Carmenza E. , Benavides Pablo , Navarro-Escalante Lucio
Published: July 2021

The coffee berry borer (CBB); Hypothenemus hampei (Coleoptera: Curculionidae), is widely recognized as the major insect pest of coffee crops. Like many other arthropods, CBB harbors numerous bacteria species that may have important physiological roles in host nutrition, detoxification, immunity and protection. To date, the structure and dynamics of the gut-associated bacterial community across the CBB life cycle is not yet well understood. A better understanding of the complex relationship between CBB and its bacterial companions may provide new opportunities for insect control. In the current investigation, we analyzed the diversity and abundance of gut microbiota across the CBB developmental stages under field conditions by using high-throughput Illumina sequencing of the 16S ribosomal RNA gene. Overall, 15 bacterial phyla, 38 classes, 61 orders, 101 families and 177 genera were identified across all life stages, including egg, larva 1, larva 2, pupa, and adults (female and male). Proteobacteria and Firmicutes phyla dominated the microbiota along the entire insect life cycle. Among the 177 genera, the 10 most abundant were members of Ochrobactrum (15.1%), Pantoea (6.6%), Erwinia (5.7%), Lactobacillus (4.3%), Acinetobacter (3.4%), Stenotrophomonas (3.1%), Akkermansia (3.0%), Agrobacterium (2.9%), Curtobacterium (2.7%), and Clostridium (2.7%). We found that the overall bacterial composition is diverse, variable within each life stage and appears to vary across development. About 20% of the identified OTUs were shared across all life stages, from which 28 OTUs were consistently found in all life stage replicates. Among these OTUs there are members of genera Pantoea, Erwinia, Agrobacterium, Ochrobactrum, Pseudomonas, Acinetobacter, Brachybacterium, Sphingomonas and Methylobacterium, which can be considered as the gut-associated core microbiota of H. hampei. Our findings bring additional data to enrich the understanding of gut microbiota in CBB and its possible use for development of insect control strategies.
Genomic Analysis of Sarcomyxa edulis Reveals the Basis of Its Medicinal Properties and Evolutionary Relationships
Authors: Tian Fenghua , Li Changtian , Li Yu
Published: July 2021

Yuanmo [Sarcomyxa edulis (Y.C. Dai, Niemelä & G.F. Qin) T. Saito, Tonouchi & T. Harada] is an important edible and medicinal mushroom endemic to Northeastern China. Here we report the de novo sequencing and assembly of the S. edulis genome using single-molecule real-time sequencing technology. The whole genome was approximately 35.65 Mb, with a G + C content of 48.31%. Genome assembly generated 41 contigs with an N50 length of 1,772,559 bp. The genome comprised 9,364 annotated protein-coding genes, many of which encoded enzymes involved in the modification, biosynthesis, and degradation of glycoconjugates and carbohydrates or enzymes predicted to be involved in the biosynthesis of secondary metabolites such as terpene, type I polyketide, siderophore, and fatty acids, which are responsible for the pharmacodynamic activities of S. edulis. We also identified genes encoding 1,3-β-glucan synthase and endo-1,3(4)-β-glucanase, which are involved in polysaccharide and uridine diphosphate glucose biosynthesis. Phylogenetic and comparative analyses of Basidiomycota fungi based on a single-copy orthologous protein indicated that the Sarcomyxa genus is an independent group that evolved from the Pleurotaceae family. The annotated whole-genome sequence of S. edulis can serve as a reference for investigations of bioactive compounds with medicinal value and the development and commercial production of superior S. edulis varieties.