bluuebunny/crossref_metadata_2025 huggingface dataset:
ID:2030 Malnutrition and Quality of Life in Patients with Non-Small-Cell Lung Cancer at Thai Nguyen Cancer center
Authors: Nguyen Hoang Cong , Ngo Tinh Thi , Hoang Cuong Quoc
Published: September 2017

Nowadays Progressive weight loss, common reduces performance and quality of life in patients with advanced lung cancer. However, there is a paucity of studies that focus on nutritional status and quality of life of non-small cell lung cancer (NSCLC) patients. The present study seeks to determine the nutritional status, and its relation to quality of life, of NSCLC patients. One hundred NSCLC patients (mean age 60,1 years) were evaluated during therapy at the Lower Silesian Center of Lung Diseases in Thai Nguyen – Vietnam. Nutritional status was evaluated by means of the Mini-Nutritional Assessment (MNA) and quality of life by means of two instruments developed by the European Organization for the Research and Treatment of Cancer (EORTC): QLQ-C30 and QLQ-LC13 questionnaires. The MNA revealed that up to 49,1% of patients were undernourished, 24,9% were at risk of malnutrition, and only 26.0% showed a normal nutrition. The well-nourished respondents evaluated their quality of life better in all functional scaQLQ-C30 and specific LC13 questionnaires. In univariate analysis, malnutrition significantly correlated with decreased quality of life and the intensity of symptoms in both questionnaires. In multivariate analysis, malnutrition was an independent determinant of decreased quality of life in physical functioning domain (β ¼ 0.015; p < 0.001). We conclude that malnutrition has an impact on quality of life and on the presentation of symptoms in NSCLC patients. Therefore, nutritional care should be integrated into the global oncology as an adjunct to symptomatic treatment. les (33.3 vs. 41.7 vs. 66.7, respectively) and presented less intensive symptoms in general, research has contributed to the current viewpoint cancer treatment.
ID2033 Precision-cut tissue slices as a novel ex-vivo model for evaluating the efficacy of potential drugs
Authors: Iswandana Raditya , Pham Bao Tung , Luangmonkon Theerut , Mutsaers Henricus A.M. , Olinga Peter
Published: September 2017

Background: Recently, we developed a novel model for drug screening by culturing ex-vivo precision-cut tissue slices (PCTS). The tissue slice consists of multiple cell types still in their normal matrix environment and structure provides numerous advantages compare to other models. Our objective was to use this model to investigate the effect of various potential compounds. In this study precision-cut intestinal slices (PCIS) were used to evaluate some transforming growth factor (TGF- β) and platelet-derived growth factor (PDGF) pathway inhibitors. TGF-β and PDGF are key cytokines in fibrotic and cancer diseases and are the main targets for treatment.

Methods: Murine PCIS were cultured for 48 h in the presence of profibrotic and/or antifibrotic compounds. The fibrotic process was studied on gene and protein level using a variety of markers including (pro)collagen 1a1 (Col1a1), heat shock protein 47 (Hsp47), fibronectin (Fn2) and plasminogen activator inhibitor-1 (PAI-1). The effects of potential drugs mainly inhibiting the TGFb pathway i.e. valproic acid, tetrandrine, pirfenidone, SB203580 and LY2109761 as well as compounds mainly acting on the PDGF pathway i.e. imatinib, sorafenib and sunitinib were assessed in the model at maximum non-toxic concentrations.

Results: Murine PCIS remained viable for 48 h and the onset of fibrosis was observed during culture, as demonstrated by an increased expression of, amongst others, Hsp47, Fn2 and Pai-1. Furthermore, TGFb1 stimulated fibrogenesis while PDGF had no effect. Regarding the tested antifibrotics, pirfenidone, LY2109761 and sunitinib had the most pronounced impact on fibrogenesis, both in the absence and presence of profibrotic factors, as illustrated by reduced levels of Col1a1, Hsp47, Fn2 and Pai-1 following treatment. Moreover, LY2109761 significantly reduced fibronectin protein expression in the presence of TGFb1.

Conclusion: PCIS can successfully be used to test drug efficacy. Using the model we demonstrated that tetrandrine, pirfenidone, LY2109761 and sunitinib showed potential antifibrotic effects on a gene level, warranting further evaluation of these compounds for the treatment of fibrosis disease. By using tissue extracted from patient, PCIS could also be a promising model to screen drug for personalized treatment in fibrotic and cancer disease.
ID:2034 Structural and Functional Polymorphism in Hepatitis B Virus Oncogene, HBx
Authors: Jabeen Nusrat , Hussain Mushtaq
Published: September 2017

Background: HBV is now firmly associated with the incidence of Hepatocellular Carcinoma. This oncogenic potential of HBV is primarily due to an oncogene referred to as HBx. The present study deals with the sequential and structural variations found in the HBx protein encoded by the genomes of different HBV genotypes.

 

Methods: In total 5991 sequences of HBx belonging to different genotypes of HBV were aligned to procure consensus sequences of each genotype. The sequence was than used to construct molecular structure of HBx employing iterative threading alignment. The models were optimized and refined for the structural and thermodynamic parameters. The selected models were used to develop structures of HBx of each genotype which were in turn compared for their structural attributes, dimer formation and molecular interactions with p53.

 

Results: The sequence alignment reveals considerable conservation in the HBx sequences of different genotypes. The strongly conserved C-terminal region is a component of p53 interaction region of HBx. The most diverged region was found to be dimerization region spanning 25 to 50 aa. Structurally, HBx molecule has found to be nearly disordered except the C-terminal p53 binding region that adopts helical conformation. This in turn may explain in part the promiscuous nature of HBx in terms of binding with multiple binding partners. These observations have been further verified by GlobPlot analysis. The disordered region potentially rendered variations in the intra and inter molecular complexes of HBx as observed by molecular docking analysis.

 

Conclusion: The data highlights the variations in the structural characteristics of HBx that underscores the variations in the pathological and oncogenic consequences of HBV infections rendered by different HBV genotypes.
ID:2037 Molecular mechanisms underlying resistance to MEK1/2 inhibitor in BRAF-mutated colorectal cancer
Authors: Duong Hong-Quan
Published: September 2017

Colorectal carcinomas are characterized by multiple genetic alterations, including constitutive Wnt activity and gain-of-function mutations in K-RAS and B-RAF. BRAF encodes a Ser/Thr kinase acting in the Ras/MEK/ERK pathway and the V600E mutation found in 11% of colorectal cancers renders this kinase constitutively active. B-RAF mutated colorectal carcinomas represents a very aggressive entity with a poor prognosis. Understanding the molecular mechanisms activated downstream of mutated B-RAF is urgently needed to design new therapeutic avenues to treat B-ARF mutated colorectal carcinomas and to circumvent resistance to therapies targeting the Ras/Raf/MEK1/ERK1/2 pathway. In a search for candidates that critically contribute to both intrinsic and acquired resistance to MEK1 inhibition in B-RAF mutated colorectal cancer cells, we identified one scaffold protein whose expression is driven by both NF-kB and AP-1 families of transcription factors. This scaffold protein promotes the expression of HER2 and HER3 in colorectal cancer cells subjected to MEK1 or B-RAF inhibition (Selumetinib and Vemurafenib, respectively) and, as such, is critically involved in the intrinsic resistance to these targeted therapies. The same scaffold protein is also strongly induced in B-RAF but not K-RAS mutated colorectal cancer cells showing acquired resistance to MEK1 inhibition. Interfering with the expression of this scaffold protein circumvents both intrinsic and acquired resistance to Selumetinib in B-RAF mutated colorectal cancer cells. Our study defines a new molecular actor critically involved in oncogenic signaling pathways triggered by mutated B-RAF. Our study also defineS new combinatory therapies to better treat B-RAF-mutated colorectal carcinomas.
ID:2043 Response to a 2-Indolinones laboratory based regimen analogous in cancerous-infected cells
Authors: Hajare Rahul
Published: September 2017

2-Indolinone is multifunctional expressed by drug discovery series of novel 97 compounds were synthesized for anticancer and cytotoxic activity against different 60 cell line cultures [1]. 07 compounds were selected on molecular recognition and screened for anticancer study [2]. Among those is screened compounds RH8, RH 42, RH84, RH89, RH90, RH 94, RH97 found good quality of anticancer activity against most of the cancer cell line with ranges from -77.23 to 55.85% growth. Target compound 5-Flouro-1-benzyl isatin -2, 3-b-quinoxaline (RH-89) display -77.23 to 7.31% growth & another target compound RH 94 display -64.78 to 65.14% growth against most of the leukemic cell lines, while RH-08 has shown very good activity against CCRF-CEM cell line with -31.85% growths and weak anticancer activity against breast cancer cell line with 53.47% growth, but failed to respond against other leukemic cell lines. RH-89 has also shown moderate activity against non-small cell-lung cancer (HOP-62) with 34.69% growth. RH-90 has shown good anticancer activity against leukemic cell line SR with 18.70% growth and weak activity against Non small cell lung cancer HOP-92 and NCL-H322M with 57.14 and 57.20% growth respectively. The cytotoxic property [3] of 2-indolinone analogs, among 3-benzohydrazide (RH 06-64) series, RH-22 and 28 were found to be most cytotoxic against L-1210 cell line (9 and 6 µg/ml). RH-33 was found to be cytotoxic against CEM/0 cell line (6.9 µg/ml). Among 3-isonicotinohydrazide derivatives (RH 65-72), RH-70 was found to have better cytotoxic property against all three types of cell lines (6.1-6.7 µg/ml). 

From 3-quinazolin-4(3H)-one series (RH 74-82), RH-75 was found to be better cytotoxic against Molt4/8 cell line (6.0 µg/ml) and RH-77 was found to have better cytotoxicity against L1210 cell line (9µg/ml) remaining compounds RH-79, 80, 82 have shown moderate to weak cytotoxic property. Among 6H-indolo [2,3-b]quinoxaline derivatives (RH 83-96), RH-92 have shown better cytotoxic property against L1210 cell line(7.2 µg/ml).
ID:2048 Computational Methods for Personalized Medicine in Cancer Research
Authors: Le Duc-Hau
Published: September 2017

Personalized medicine is a medical procedure that stratify patients into different groups with specific treatments relied on their predicted response or risk of disease. To support researches in personalized medicine and manufacture viral vaccines and other products of biotechnology (e.g., drug), both tumors and cell lines, which are grown from the tumors, are used. With rapid growth of biomedical and clinical data, many computational methods have been proposed to identify candidate disease-associated cellular components, to predict novel targets of drugs, to predict response of drugs, or to repurpose the use of drug for other diseases, etc.… Specially for personalized medicine, computational methods have been proposed to classify patients into different subtypes according to genomic, epigenomic profiles and/or drug responses on cell lines or patient tumors [1-5], and therefore able to predict response of untested drugs [6], drug synergy [7] as well as to identify predictive genomic features and treatment for each group of patients. Among diseases, cancer is a complex genetic disease and has been received most focus in personalized medicine research [8]. In addition, many data resources for are publicly available for the research such as CCLE [9], COSMIC [10], TCGA [11] and GDSC [12].
ID:2052 MANUFACTURING BIOLOGICAL MEMBRANE FROM BOVINE PERICARDIUM EXTRACELLULAR MATRIX TOWARD THE PERIODONTAL APPLICATION
Authors: Nguyen Thi Ngoc My
Published: September 2017

Collagen membrane that was mainly from the bovine and porcine pericardium has been studied and widely applied for most manufacturers of bioprothetic materials, especially periodontal therapeutics. As many studied, bovine pericardium has higher collagen content than that from porcine, although both membranes have no significant different in calcification when treated with glutaraldehyde. In this study, acellular bovine was used to fabricated a biomembrane for periodontal reconstruction and regeneration. Acellular bovine pericardium was treated with glutaraldehyde at different concentrations and time points. The treatment of 0,1% glutaraldehyde for 6 hours was determined as the optimal fabrication protocol according to membrane properties, including thickness (0.2mm-0.5mm), tensile strength (5MPa-12MPa), tensile strain (15%-50%), around 5µm pore size, limited in vitro degradation (1%-5%), which met all criteria parameters for periodontal treatment. The fabricated membrane presented in vitro biocompatibility to human fibroblasts according to ISO10993-5. Moreover, the membrane promoted the in vitro migration of humane gingival fibroblasts, which indicated the application as a guided biomembrane in periodontal reconstuction and regeneration.
ID: 1008 Quercus infectoria semi-purified fractions promoted BMP-2, Runx2 and osteopontin expression in human fetal osteoblastic cell line
Authors: Abdullah Amira Raudhah , Hapidin Hermizi , Abdullah Hasmah
Published: September 2017

Quercus infectoria (QI) is traditional medicines in Asia which contain many active phytochemical compounds that have the potential to stimulate bone formation. However, the precise mechanism of the therapeutic effect of QI on osteoblast has not been elucidated. The present study was carried out to extract the active compound of QI through a series of purification in order to obtain semi-purified fractions of QI followed by examining its phytochemical profile through liquid chromatography mass spectrometry (LC-MS) as well as to delineate the molecular mechanism of QI semi-purified fraction-enhanced bone formation by investigating the protein expression of bone morphogenic protein-2 (BMP-2), Runx2 and osteopontin (OPN) in the hFOB 1.19 human fetal osteoblastic cell line. The semi-purified fractions of QI were produced in series of column chromatography and thin layer chromatography (TLC) technique. The obtained fractions were then tested in a series of bio-guided assay through MTT assay. Three most potent fractions with lowest EC50 value (Fraction A-10.85 µg/ml, Fraction B-12.00 µg/ml and Fraction C-11.60 µg/ml) were selected to be treated with hFOB1.19 cell followed by quantification of BMP-2, Runx2 and OPN protein expression at day 1, 3 and 7. Compound profiling through LC-MS analysis showed that the chosen fractions consist of main active component known as gallic acid that has been showed previously to promote bone formation. Results show that QI semi-purified fractions increased the activity of BMP-2, Runx2 and OPN at day 1 and day 3. Meanwhile as the expression of BMP-2 and Runx2 continue to increase at day 7, the expression of OPN shows a down regulation at day 7. Furthermore, the study showed that combination of Fraction A, B and C wit osteoporotic drug (pamidronate) further increase the expression of BMP-2 and Runx2 contrary to OPN expression. These finding demonstrated that semi-purified fraction of QI enhanced bone formation through expression of investigated bone-related marker.
ID: 1014 Expanded autologous adipose derived stem cell transplantation for type 2 diabetes mellitus
Authors: Le Phuong Thi-Bich , Pham Phuc Van , Vu Ngoc Bich , Dang Loan Thi-Tung , Phan Ngoc Kim
Published: September 2017

Introduction: Type 2 diabetes mellitus (T2D) is the most common form of diabetes mellitus, accounting for 90% of diabetes mellitus in patients. At the present time, although T2D can be treated by various drugs and therapies using insulin replacement, reports have shown that complications including microvascular, macrovascular complications and therapy resistance can occur in patients on long term treatment. Stem cell therapy is regarded as a promising therapy for diabetes mellitus, including T2D. The aim of this study was to evaluate the safety and therapeutic effect of expanded autologous adipose derived stem cell (ADSC) transplantation for T2D treatment; the pilot study included 3 patients who were followed for 3 months. Methods: The ADSCs were isolated from stromal vascular fractions, harvested from the belly of the patient,and expanded for 21 days per previously published studies. Before transplantation, ADSCs were evaluated for endotoxin, mycoplasma contamination, and karyotype. All patients were transfused with ADSCs at 1-2x106 cells/kg of body weight.Patients were evaluated for criteria related to transplantation safety and therapeutic effects; these included fever, blood glucose level before transplantation of ADSCs, and blood glucose level after transplantation (at 1, 2 and 3 months). Results: The results showed that all samples of ADSCs exhibited the MSC phenotype with stable karyotype (2n=46), there was no contamination of mycoplasma, and endotoxin levels were low (<0.25 EU/mL). No adverse effects were detected after 3 months of transplantation. Decreases of blood glucose levels were recorded in all patients. Conclusion: The findings from this initial study show that expanded autologous ADSCs may be a promising treatment for T2D.
ID: 1015 Taraxacum officinale (dandelion) extract efficiently inhibited the breast cancer stem cell proliferation
Authors: Trinh Ngu Van , Dang Nghi Doan-Phuong , Tran Diem Hong , Pham Phuc Van
Published: September 2017

Introduction:

Breast cancer stem cells (BCSCs) play an important role in breast cancer initiation, metastasis, recurrence, and drug resistance. Therefore, targeting BCSCs is an essential strategy to suppress cancer growth. This study aimed to evaluate the effects of dandelion Taraxacum officinale extracts on BCSC proliferation in vitro in 2D and 3D cell culture platforms.

Methods:

The BCSCs were maintained understandard conditions, verified for expression of CD44 and CD24 surface markers, and transfected with GFP before use in experiments. In the 2D model, the BCSCs were cultured as adherent cells in standard culture plates; in the 3D model, the BCSCs were cultured on low-adherent plates to form spheroids. The effect of Dandelion extracts on proliferation of BCSC was assessed by evaluating induction of cell death, expression of genes of death receptor signaling pathways, and production of reactive oxygen species (ROS) by BCSCs.

Results:

BCSCs formed spheroids as microtumors in vitro and exhibited some in vivo characteristics of tumors, such as increased expression of N-cadherin and Slug, decreased expression of E-cadherin, capacity to invade into the extracellular matrix (ECM), and presence of a hypoxic environment at the core of tumor spheroids. The dandelion extracts significantly inhibited BCSC proliferation in both two-dimensional (2D) and three-dimensional (3D) models of BCSCs. However, the IC50 value of dandelion extracts in BCSCs in the 3D model was much higher than that in the 2D model. The results also demonstrated that BCSCs treated with Dandelion extracts showed increased expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and TRAIL receptor 2 (TRAILR2; i.e. death receptor 5;DR5). Moreover, treatment induced expression of DR4. Treatment with methanol dandelion extract enhanced production of ROS in BCSCs.

 Conclusion:

Dandelion extracts are promising extracts for the treatment of breast tumors. The effect of methanol dandelion extract was better than that for ethanol extract. Importantly, BCSCs in 3D exhibited stronger drug resistance than those in 2D. In summary, our results indicate the strong potential of dandelion extracts as anti-cancer agents and rational use for drug development.
ID: 1023 All-trans retinoic acid targets gastric cancer stem cells and inhibits patient-derived gastric carcinoma tumor growth
Authors: Nguyen P H , Giraud J , Staedel C , Chambonnier L , Dubus P , Chevret E , Boeuf H , Gauthereau X , Rousseau B , Fevre M , Soubeyran I , Belleannée G , Evrad S , Collet D , Mégraud F , Varon C
Published: September 2017

Gastric carcinoma is the third leading cause of cancer-related death worldwide. This cancer, most of the time metastatic, is essentially treated by surgery associated with conventional chemotherapy, and has a poor prognosis. The existence of cancer stem cells (CSC) expressing CD44 and a high aldehyde dehydrogenase (ALDH) activity has recently been demonstrated in gastric carcinoma and has opened new perspectives to develop targeted therapy. In this study, we evaluated the effects of all-transretinoic acid (ATRA) on CSCs in human gastric carcinoma. ATRA effects were evaluated on the proliferation and tumorigenic properties of gastric carcinoma cells from patient-derived tumors and cell lines in conventional 2D cultures, in 3D culture systems (tumorsphere assay) and in mouse xenograft models. ATRA inhibited both tumorspheres initiation and growth in vitro, which was associated with a cell-cycle arrest through the upregulation of cyclin-dependent kinase (CDK) inhibitors and the downregulation of

cell-cycle progression activators. More importantly, ATRA downregulated the expression of the CSC markers CD44 and ALDH as well as stemness genes such as Klf4 and Sox2 and induced differentiation of tumorspheres. Finally, 2 weeks of daily ATRA treatment were sufficient to inhibit gastric tumor progression in vivo, which was associated with a decrease in CD44, ALDH1, Ki67 and PCNA expression in the remaining tumor cells. Administration of ATRA appears to be a potent strategy to efficiently inhibit tumor growth and more importantly to target gastric CSCs in both intestinal and diffuse types of gastric carcinoma.
ID: 1026 Reconstruction of the major defects of the tissue scalp
Authors: Muradov M.I. , Mukhamedkerim K.B. , ABaiguzeva A. , Kazantaev K.E. , Koshkarbaev D.Zh.
Published: September 2017

Background: To provide quantitative objective data demonstrating the longevity and amount of volume augmentation in the fatty dystrophy of the facial tissue obtained with autologous lipofilling.

 

Methods: In our clinic had been operated 8 patients for last 2 years with fatty dystrophy of the facial tissue. A prospective analysis of all patients who underwent at our private practice and were followed up for at least 1,5 year. Surgery was performed under general anesthesia it is necessary for clear results tissue correction. We based on the literature has seen numerous clinical reports highlighting the benefits of autologous fat transfer for face from that areas, fat was collected from the abdomen (most frequently used donor site), hips, outer thighs (saddle-bags), internal knee or thigh, with quantitative volume measurements evaluating the amount of postoperative volume change.

 

Results: Twenty eight patients were included in the study. The mean follow-up time was 18 months. The mean amount of autologous fat injected into each face region was 10-70 mL. Hypercorrection was performed after 3 months and it was 20-50% of the initial injected fat volume. Overall, the mean absolute volume augmentation measured at their last (after 6 month) post operative visit was 10-25%. There was variability between patients in the volume amount and percentage that remained. The resorption process was observed in two patients after 6 month. We made correction with hyaluronic acid and silicone implants.

 

Conclusion: To our knowledge, this study is the first clinical quantification in our practise of autologous fat transfer and/or grafting in the literature that provides definitive evidence on the amount as well as the resultant longevity in the face. Autologous fat transfer to the face has definite long-term volume augmentation results. On average, approximately 25-35% of the injected volume remains at 18 months. However, some variability exists in the percentage of  volume that remains that may require a touch-up procedure.
ID: 1027 High-Throughput, Single Cell Whole Transcriptome Sequencing Analysis of Cancer Cells with the New BD FACSMelody™ Cell Sorter and BD™ Precise assay
Authors: Wang Xiaoyang (Alice) , Lomas Chip , Betts Craig , Walker Aaron , Fan Christina , Weaver Suzanne
Published: September 2017

Gene expression studies performed on bulk samples might obscure the understanding of complex samples. Gene expression analyses performed on single cells, however, can offer a powerful method to resolve sample heterogeneity and reveal hidden biology. Optimal sample preparation is critical to obtain high quality gene expression data from single cells.Historically, single cells or small numbers of cells were isolated and prepared by limiting dilutions, laser capture microdissection, or microfluidics technologies, or fluorescence-activated cell sorting (FACS). FACS sorting enables highthroughput processing of a heterogeneous mixture of cells and ensures the delivery of single cells or a small number ofcells into a chosen receptacle to meet the selection criteria at a purity level that is unmatched by other approaches.Furthermore, by FACS, the single cell selection criteria can be based on surface marker expression, cell size, and granularity(represented by scatter). Sorted cells can be used for any downstream application including next generation sequencing(NGS).In this study, the new, easy-to-use BD FACSMelody™ sorter was applied to sort individual cancer cells. Jurkat cells (a Tleukemia cell line), and T47D cells (a breast cancer cell line) were mixed, stained, analyzed, and sorted on a BD FACSMelody system. The individual cell’s whole transcriptome was interrogated using BD™ Precise Single Cell WTA (whole transcriptome amplification) Assay. Principal component analysis was applied to cluster the sorted Jurkat and T47D-cell populations.
ID: 1029 Effects of carnosine on regulation of migration and invasion in human colorectal cancer cells
Authors: Lai Po-Yu , Hsieh Shu-Chen , Wu Chih-Chung , Hsieh Shu-Ling
Published: September 2017

Colorectal cancer is the third most commonly diagnosed cancer in the word. Carnosine is an endogenous dipeptide found in vertebrate skeletal muscles. It is known to have anti-fatigue, antioxidative, antihypertensive, antidiabetic, and cancer inhibiting effects. However, little research has been done regarding its influence on the metastasis of colon cancer. This study cultivated HCT-116 human colon cancer cells as a test model in order to investigate the impact of carnosine on the migration and invasion of human colon cancer cells. The results showed that 48-hour treatments of HCT-116 cells with 0.5, 1, or 5 mM carnosine each significantly inhibited the migration ability of the cells (P < 0.05). The 48-hour treatments with 0.5, 1, or 5 mM carnosine were also found to significantly reduce MMP-9 activity (P < 0.05), but not MMP-2 expression. Furthermore, when HCT-116 cells treated with 1 or 5 mM carnosine, invasion ability are significantly decreased and significantly increased E-cadherin expression (P < 0.05). On the other hand, the protein of TIMP-1, an inhibitor of MMP-9, is signification increased after 1 or 5 mM carnosine treatment (P<0.05). In addition, the u-PA protein level are significantly decreased after carnosine treatment.

The results indicate that carnosine can regulate the migration and invasion by regulating MMPs and its regulator molecular expression in HCT-116 cells.
ID: 1031 Synthesis of Polymeric Nanoparticles of α-mangostin and its Cytotoxicity to Human Cancer Cell Lines
Authors: Nguyen Phuong T.M. , Tran Lam D. , Dang Nguyen K. , Tran Mai T. , Nguyen Anh V.T. , Bolhuis Albert
Published: September 2017

α-Mangostin (AMG) extracted from Garcinia mangostana L., has potent anti-cancer properties, however its clinical application is limited due to poor aqueous-solubility. Here, we successfully synthesised and characterised water-soluble ß-cyclodextrin-coated AMG 30-50 nm-size nanoparticles (NMG). Similar cytotoxic activities against LU-1 (human lung adenocarcinoma) and HL-60 (human promyelocytic leukemia) cancer cells of water-soluble NMG and organic-solvent soluble AMG were found with IC50 of 8.86 and 9.86 µg/mL, respectively. The data demonstrates that the nanoparticles improve bioavailability while maintain anti-cancer activity of AMG.
ID: 1033 Case report: Results of the first case of Stem cell transplantation from cord blood cryopreservation for β-thalassemia patient at Vinmec International Hospital
Authors: NT Phu , Dam Phuong T.M. , NT Dung , PT Lan , NT Liem
Published: September 2017

Background: To report the result of first case of β thalassemia, which is successfully transplanted by using allogeneic cord blood at the Vinmec International Hospital.

 Methods: Male patient, born in January 2014, was diagnosed with β thalassemia at 3 months of age. Patient was received cord blood transplantation from his younger sibling who matches HLA index of 10/10 in August 2016.

 Results: The number of cells in cord blood met high quality and after 30 days of transplantation, engraftment of patient fully developed with the Chimerism test was 100%. After 3 months of follow-up, it was demonstrated that the transplantation was successful.

 Conclusion: We present an effective case of hematopoietic stem cell transplantation from human umbilical cord blood of sibling for treatment of thalassemia.
ID: 1037 Effect of fluorescent nanodiamonds on umbilical cord mesenchymal stem cell differentiation into hepatocyte-like cell
Authors: Do Trung Kien , Nga Nguyen Thi Thanh , Anh Nguyen Quynh , Pham Dinh Minh , Ha Chu Hoang
Published: September 2017

Fluorescent nanodiamond (FND) indicated that it has excellent biocompatibility and photostability,so it well suited for long-term labeling and tracking of stem cells. There are many reports concerning the factors controlling stem cell differentiation. However, still little knowledge about the biomaterials properties influence stem cell alive, growth and differentiation processing. In this study, we evaluate the effect of fluorescent nanodiamond in in vitro culture and differentiation of ucMSC into hepatocyte-like cell. Mesenchymal stem cells (MSCs) were isolated from the umbilical cord (UC) and CD markers were analyzed by flow cytometry and genes expression. For hepatic differentiation of UC-MSCs, cells were induced with HGF and DMSO treated. FND was supply in the experimental group which 10 g/ml in 4 hours. The FND uptake was detected of fluorescence intensity of FND in cells by flow cytometry and laser scan microscopy. The effect of FND into UCMSCs was not only evaluated by the cell alive and growth assay but also effective differentiation throughout morphology charging or gene expression levels of AFP, ALB, and HNF4 were determined by RT-PCR and real-time PCR. The result showed that the FND was well uptake in UCMSCs. It was no affected into ability of the cell alive and growth. The existence of FNDs does not disturb the functions of UC-MSCs differentiation into hepatocyte-like cell. FND can be utilized for the labeling and tracking of UC-MSCs and hepatocyte-like cell in homing research.
ID: 1041 Effectiveness of human adipose-derived stem cell therapy pretreated with hepatocyte growth factor in liver fibrosis mouse model
Authors: Vo Ngoc Hong , Le Trinh Van , Nguyen Nam Hai , Nguyen Yen K.T. , Dang Thanh Minh , Do Huy Quang
Published: September 2017

Background: Adipose-derived stem cells (ADSCs) have the potential therapeutic impact on the liver fibrosis. Hepatocyte growth factor (HGF) is pivotal for damage repair with its anti-apoptotic, anti-fibrotic and cell migration-promoting effect. In this study, the therapy with HGF-pretreated hADSCs was expected to enhance the therapeutic effect in the amelioration of liver fibrosis compared with untreated hADSCs.

 

Method: HGF-hADSCs were prepared by culturing hADSCs for seven days in the medium added HGF.

HGF-hADSCs transplantation was performed to investigate the therapeutic effect of these cells on carbon tetrachloride (CCl4)-induced liver fibrosis in a mouse model. 

Results: After seven days and fourteen days of cell transfusion, HGF-hADSCs ameliorated the liver fibrosis. The results showed the attenuation of the liver injury (ALT level), the down-regulation of procollagen-1 (7 days, 3-fold; 14 days, 6.7-fold) and alpha -smooth muscle actin (alpha-SMA) expression (7 days, 10-fold; 14 days, 2-fold), and the histological improvement. Notably, there was the significant difference in the procollagen-1 between HGF-hADSCs and untreated hADSCs groups. Thus, the therapy with HGF-hADSCs was more efficient in the liver fibrosis treatment compared with untreated hADSCs. 

Conclusion: HGF pretreated hADSCs have a potential therapy in the treatment of liver fibrosis
ID: 1046 Antifungal prophylaxis in hematopoietic stem cell transplantation recipients: drug use evaluation at Vietnam National Institute of Hemotology and Blood transfusion
Authors: T. Son Nguyen , Nguyen Hoa M. , Nguyen Anh H. , Nguyen Tan D. , Vo Binh T.T.
Published: September 2017

*For correspondence:

tungson94@gmail.com

 

Competing interests: The authors declare that no competing interests exist.

Received: 2017-07-13 

Accepted: 2017-08-04

Published: 2017-09-05

 

Copyright The Author(s) 2017. This article is published with open access by BioMedPress (BMP).

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.    

Background: Hematopoietic stem cell transplantation (HSCT) recipients were known to be in high risk of fungal infection. However, not all HSCT patients need antifungal prophylaxis. The aim of this study was to describe the characteristics of the use of antifungal prophylaxis in hematopoietic stem cell transplantation recipients and to evaluate the appropriateness of use.

 Methods: In this observational prospective study, adult hematopoietic stem cell transplantation recipients without using antifungal treatment were recruited from start of stem cell transfusion in Vietnam National Institute of Hematology and Blood Transfusion (from April to December 2016) to end of antifungal prophylaxis or start of antifungal treatment. Appropriateness was defined based on National Comprehensive Cancer Network (NCCN) guidelines. Clinically significant Drug-drug interactions related to antifungals were defined based on Hansten and Horn’s Drug interaction and management 2013.

 Results: 38 patients (mean age: 35.7 ± 12.0; 57.9% male) were admitted, included 21 autologous and 17 allogeneic HSCT recipients. After HSCT, 3 deaths, 1 to ICU, 1 IFI. Azoles prophylaxis was given to 100% of patients with median 17.5 days (13 - 47.5). Fluconazole was the most used antifungal agents, in 94.7% of patients and 74.5% of episodes. 10 patients had been changed antifungal agents. Indication was appropriate in 44.7%, 0% in autologous and 100% in allogeneic group. In patients with appropriate indication, choice of antifungal agents was 100% appropriate. 100% of patients used inappropriate dosage, mostly lower than recommeded dosage with azoles. 12.7% had appropriate prophylactic time. Potential drug-drug interactions were identified in 92.1% of patients. Most frequent interactions involved azoles-diazepam (80.4% of episodes) and azoles-immunosuppressive drugs (49% of episodes).

 Conclusion: Our evaluation revealed a high proportion of inappropriate of antifungal prophylaxis. An antifungal stewardship programme is needed to strengthen rational use of antifungal in this specific circumstance.
ID: 1047 1. National Comprehensive Cancer Network (2015), "Prevention and Treatment of Cancer-Related Infections (Version 2.2015)". 2. National Comprehensive Cancer Network (2016), "Prevention and Treatment of Cancer-Related Infections (Version 2.2016)". 3. Hanste
Authors: Chi Bao Bui , Huynh Nga Nguyen Thi , Minh Hiep Nguyen , Diem My Vu , Ngoc Duy Pham , Bach Yen Pham Thi , Huy Nam Nguyen
Published: September 2017

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a hereditary disorder of the cardiac muscle characterised by ventricular arrhythmias, cardiac failure and sudden cardiac death. Desmosomes - the intercellular junctions of both epithelial and cardiovascular tissues that connect intermediate filaments of adjacent cells, generating a large and mechanically resilient network - are disordered in ARVC. Here, we exploit new insights into desmoplakin (DP), a critical component of desmosome structures. Indeed, both patient skin and keratinocytes expressing DP mutant construct showed large intercellular aggregates and a decrease in the amount of junctional proteins at areas of cell-cell contact. Moreover, experiments with DP knockout mice indicated that mislocalization of another junctional protein, connexin 43 was ameliorated by b-blocker (beta-blocker), or b-adrenergic receptor blocker - known to interfere with the binding to the receptor of epinephrine and other stress hormones to weaken the effects of stress hormones. Thus, these novel findings fortify the genetic and cellular mechanisms behind the marked heterogeneity of the disease and provide new therapeutic interventions that target intercellular junctions.
ID: 1049 Variation of mitochondrial DNA HV1 and HV2 of the Vietnamese population
Authors: Quy Linh Nguyen , Pham Le Anh Tuan , Trinh Anh Hong , Nguyen Thuy Thi , Tran Thinh Huy , Tran Anh Van , Nguyen Hinh Duc , Ta Van Thanh , Tran Van Khanh
Published: September 2017

The sequence polymorphism of mitochondrial DNA (mtDNA) hypervariable Segment 1 (HV1) and hypervariable Segment 2 (HV2) are studied and applied to genetic diversity and human evolution assessment, forensic genetics, consanguinity determination, and mitochondrial disease diagnosis.

Nucleotide sequence variations in HV1 and HV2, two hypervariable segments of the noncoding control region of human mitochondrial DNA (mtDNA), in selected ethnics of the Vietnamese population were elucidated through sequencing. In this study, we define the variations of HV1 and HV2 of 517 unrelated Vietnamese individuals in Kinh, Muong, Cham, and Khmer ethnic. We found 50 haplogroups: F1a haplogroup frequency is the highest at 15.7%; B5a haplogroup frequency is 10.8%, M haplogroup frequency is 8.9%, M7b1 haplogroup frequency is 7.7%; B6, D4e, D5a, E, F1c, F2a, F3a, G2a, M9b, N, N21 and U5a haplogroup frequencies are the lowest (1%). The frequency of SNP A263G are 100%; A73G is 99.6%, 315insC is 96%; 309insC is 56%; C16223T is 41%, and T16189C is 39%. We have assessed the genetic polymorphism of mtDNA HV1 and HV2 of 517 Kinh, Muong, Cham, Khmer ethnic samples.
ID: 1053 Characterisation, proliferation and differentiation potential of long term cultured Wharton’s Jelly derived mesenchymal stem cells
Authors: Ajak Warda Abdul , Simat Siti Fatimah , Eng How Siew , Lasimbang Helen Benedict , Lin Teoh Peik
Published: September 2017

Background:Mesenchymal stem cells (MSCs) have a promising role in regenerative medicine with their self-renewal and multilineage differentiation abilities. However, cell expansion is essential before their application and reports have showed that long term culture of MSCs can alter their stem cell characteristics. Wharton’s jelly derived MSCs (WJ-MSCs) as favorable source of MSCs need to be examined in long term culture before used in clinical settings. In this study, WJ-MSCs were isolated via enzymatic digestion using collagenase type 1. Cells at P5, P10 and P15 were observed for their morphology and growth kinetics where the findings showed that the extensive culture of WJ-MSCs can reach an average of 40 population doubling time with slight changes in their fibroblast-like morphology. The analysis of clonogenic activity showed no significant difference in WJ-MSCs’ ability in forming colony at early passage and later passage. Oil Red O and Von Kossa staining results for in vitro differentiation assays of WJ-MSCs into adipocytes and osteocytes showed WJ-MSCs were easily differentiated at P5 compared to P15. The reduction in both proliferation and differentiation potentials of WJ-MSCs were observed at later passages (P15). These suggested that as the passage numbers increases cells loss the ability in maintaining their plasticity. In conclusion, long term culture of WJ-MSCs can impair their stem cell properties therefore improvement in culture method to maintain these properties is essential
ID: 1055 Adipose-derived stem cells and platelet rich plasma ameliorate liver cirrhotic circumstance in CCl4-induced mice: a new approach for liver cirrhosis treatment
Authors: Truong Nhung Hai , Nguyen Nam Hai , Le Trinh Van , Huynh Nghia , Ngo Dat Quoc , Nguyen Thanh Van , Phan Ngoc Kim , Pham Phuc Van
Published: September 2017

Background: Stem cell therapy in liver cirrhosis treatment is attracting the attention of the scientific community. Adipose tissue-derived mesenchymal stem cells are a potential source of cells because they have self-renewal, high proliferation, and differentiation into a variety of cell types, including hepatocytes as potential cell sources for cirrhosis treatment. Platelet-rich plasma (PRP) growth factors contribute to regeneration and wound healing. We test the hypothesis that PRP co-administration enhances MSC treatment for mouse cirrhosis.

Method: Male Swiss mice were treated orally with olive oil or CCl4 for 11 weeks. PRP was obtained from healthy mice. Mouse adipose-derived stem cells (mADSCs) from adipose tissue of 3 weeks CCl4 mice were cultured for three passages (P3-mADSCs) before the transfer by tail vein injection with or without PRP into 11 weeks CCl4 mice. Mice were divided into six groups (n=10 each group). 1) normal, 2) cirrhotic, 3) cirrhotic /PBS; 4) cirrhotic/PRP (0.2 ml/mice with PRP from healthy mice), 5) cirrhotic/mADSCs (5 x 105 cells/mice), and 6) cirrhotic/mADSC-PRP.

 

Result: mADSCs were highly positive for CD44, CD90, and CD105. Relative to liver cells, P3-mADSCs highly expressed Alb, Ck18, Ck19, Tnf, c-met, Cyp1a1, Afp, Muc1, Ldl receptor. mADSCs were strongly positive for Cyp1a1 (98.21±1.57%) and Hgf (95.55±3.11%); moderately positive for alfa-fetoprotein (45.99±2.08%), Aat (44.43±7.79%), Alb (57.81±8.49%) and differentiated into hepatocyte-like cells under induction medium. After transplantation, CFDA-transplanted cells into CCl4-treated mice were found in the liver at day 21 st. Compared to mADSCs, mADSCs and PRP co-treatment most effectively improved serum AST/ALT/bilirubin and albumin levels in day seven cirrhotic mice (p<0.05); and significantly down-regulated procollagen (104-fold less) and TGF-beta 1 (10-fold less) in day 21 cirrhotic liver. Histology index and collagen deposition were improved in 100% of mADSC/PRP- and mADSCs- cirrhotic mice compared to 33.3% of PBS- or PRP- cirrhotic liver (p<0.05).

Conclusion: Cultured mADSCs express hepatocyte enriched markers. PRP coadministration enhances mADSC effects to improve liver function further, and further reduce fibrosis.
ID1056 Synthesis and biological evaluation of novel curcumin derivative with water-soluble phosphate group as potential antitumor agent
Authors: Nguyen Dinh Luyen , Nguyen Van Giang , Nong Thi Bich Van , Pham Thi Hien , Nguyen Van Hai
Published: September 2017

Background: Curcumin (diferuloylmethane) is the main natural active polyphenol from the rhizome of Curcuma longa (turmeric) and in others Curcuma spp. The main pharmacological effects include anti-tumor, anti-inflammatory, anti-oxidation, anti-fungal and anti-bacterial activities. However, the clinical application of curcumin is limited by its main drawbacks such as low solubility, poor bioavailability and rapid excretion from the body. In this study, the chemical modification approach was performed to improve the solubility of curcumin in water, at the same time increase the anti-tumor activity.

Methods: The water-soluble compound (2-(curcumin-O-yl)ethyl dihydrophosphate, LH-42017) was synthesized by two classical reactions: O-alkylation with 2-bromoethanol to obtain mono-O-(2-hydroxyethyl)-curcumin, followed by esterification using phosphoryl chloride in water and pyridine. The chemical structure was confirmed by IR-, NMR-spectroscopies and mass-spectrometry. The anti-tumor activity of LH-42017 was assessed against hepatocellular carcinoma Hep-G2, human breast cancer MCF-7, human leukemia K562 and human cervical carcinoma HeLa cell lines based on the sulforhodamine B (SRB) assay.

Results: LH-42017 was successfully synthesized from curcumin by overall yield of 28.43%. IC50 of original curcumin was 63.55, 90.88, 104.99 and 60.24 µM on Hep-G2, MCF-7, K562 and Hela, respectively. IC50 of LH-42017 was 36.82, 51.60, 60.80 and 26.14 µM on Hep-G2, MCF-7, K562 and Hela, respectively.

Conclusion: The novel synthesized compound LH-42017 showed the antitumor activity in 1.7-2.3 times better than original curcumin. The water-soluble phosphate group plays an important role in development of natural curcumin as potential antitumor agent.
ID: 1058 Effective bone formation through BMP-2-immobilized highly porous GBR membrane
Authors: Kim Min Ji , Park Jin Hyun , Kim Ho Yong , Byun June Ho , Lee Jin Ho , Oh Se Heang
Published: September 2017

Sound healing of large bone defects is critical challenges in most of clinical fields. In general healing process of bone regeneration, the rapid infiltration of connective tissue, whereas relatively slow bone regeneration in bone defect leads to the incomplete bone formation. To solve this drawback, guide bone regeneration (GBR) membrane which could prevent rapid infiltration of connective tissue into bone defect, thus GBR membrane is feasible for compact bone regeneration in clinical fields. In recent, the most researchers believed that bioactive molecules-grafted GBR membranes may enhance the bone regeneration. To allow graft of bioactive molecules from porous membrane, chemically modified scaffolds are commonly used. This modification leads to sufficient interaction with active sites of bioactive molecules to stable the immobilization of bioactive molecules in the body. However it is hard to apply to clinical applications because of the toxicity of chemical residue used for the modification. In this study, we developed a GBR membrane with leaf-stacked structure which can allow sustained release of bone morphogenetic protein-2 (BMP-2) without any additional modification. The morphology, mechanical property, BMP-2 release profile, osteogenic differentiation of human periosteum-derived cells, and new bone formation efficiency of the BMP-2-loaded GBR membrane compared with commercial product were investigated.
ID: 1059 Biomimetic release of growth factor from porous beads for enhanced bone regeneration
Authors: Kim Ho Yong , Byun June Ho , Lee Jin Ho , Oh Se Heang
Published: September 2017

Three dimensional scaffolds which allow sustained release of bioactive molecules have been considered as an essential component for enhanced bone regeneration. In recent years, combination system of various bioactive molecules has been extensively investigated to reconstruct more sophisticated bone regeneration. It is well-known that if the certain bioactive molecules with appropriate dose and stimulation periods are applied to bone defect, the physiological processes in bone regeneration may be accelerated. However, many studies were not concerned stimulation periods of bioactive molecules occurred in body during new bone formation because of absence of proper release system for the bioactive molecules. In this study, we selected three bioactive molecules (PDGF-BB, VEGF, BMP-2) which widely used for effective bone healing, and developed a porous bead with leaf-stacked structure which can allow controlled release of growth factors by concentration and composition of Pluronics. The morphology, each growth factor release profiles, migration (boyden chamber assay), angiogenic differentiation (tube formation, ICC, and RT-PCR), osteogenic differentiation (ALP activity, calcium deposition, alizarin/ALP staining, ICC, and RT-PCR) of human periosteum-derived cells and new bone formation of rat calvarial defect model by controlled release of growth factors were estimated