bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcription factor NFATc1 impairs the MDM2-p53 feedback loop to drive colorectal cancer growth
Authors: Xu Guangyao , Cao Tianyu , Zhang Jiandong , Yu Fei , Okotel Moses , Wu Mingyue , Zhang Suyang , Jin Haodong , Wan Yuting , Wu Shourong , Kasim Vivi , Huang Can
Published: July 2024

Background
Colorectal cancer (CRC) represents approximately 10% of all cancer cases and ranks as the second leading cause of cancer-related death. Existing clinical targeted therapies for CRC lack efficacy, highlighting the urgent need for identifying novel target sites.
Methods
Using the Kaplan-Meier Plotter analyzed the correlation between the expression level of NFATc1 and the prognosis/stage of CRC. Using Cell Viability Assay kit, flow cytometry, calculation of micronucleus and abnormal chromosome number, transwell system and Xenograft on nude mice, we detected the effects of NFATc1 on the cell viability, division, migration and tumor-forming ability of CRC cells. Using ChIP assay, site-directed mutagenesis and dual luciferase assay analyzed the mechanism of NFATc1 on MDM2-p53 loop. Tumor treatment with NIFE and oxaliplatin test the effect of NIFE on the anti-tumor effect of oxaliplatin.
Results
The expression level of NFATc1 in CRC tissues is significantly increased compared with that in adjacent tissues and NFATc1 expression level is closely correlated to the poor prognosis of CRC patients with advanced clinical stage. Silencing NFATc1 markedly decreased the viability, abnormal cell cycle, migration, and tumor-forming ability of CRC cells. Mechanistically, NFATc1 not only upregulate the expression of MDM2, but also disrupt the feedback activation of p53 on MDM2 by directly binding the p1 and p2 promoter regions of MDM2, thus blocking MDM2-p53 loop. Consequently, the high expression of NFATc1 leads to sustained MDM2 activation, and diminished p53 stability. Finally, nifedipine inhibited CRC tumors growth by inhibiting the NFATc1/MDM2 pathway, and significantly improve the anti-colorectal cancer effect of the clinical drug oxaliplatin.
Conclusions
NFATc1 impairs the MDM2-p53 feedback loop to drive colorectal cancer growth and Nifedipine inhibited CRC tumors growth by inhibiting the NFATc1/MDM2 pathway.
Accurate Prediction of Three-Dimensional Humanoid Avatars for
Anthropometric Modeling
Authors: Heymsfield Steven , McCarthy Cassidy , Wong Michael , Brown Jasmine , Ramirez Sophia , Yang Shengping , Bennett Jonathan , Shepherd John
Published: July 2024

Objective
To evaluate the hypothesis that anthropometric dimensions derived from a person’s manifold-regression predicted three-dimensional (3D) humanoid avatar are accurate when compared to their actual circumference, volume, and surface area measurements acquired with a ground-truth 3D optical imaging method. Avatars predicted using this approach, if accurate with respect to anthropometric dimensions, can serve multiple purposes including patient metabolic disease risk stratification in clinical settings.
Methods
Manifold regression 3D avatar prediction equations were developed on a sample of 570 adults who completed 3D optical scans, dual-energy X-ray absorptiometry (DXA), and bioimpedance analysis (BIA) evaluations. A new prospective sample of 84 adults had ground-truth measurements of 6 body circumferences, 7 volumes, and 7 surface areas with a 20-camera 3D reference scanner. 3D humanoid avatars were generated on these participants with manifold regression including age, weight, height, DXA %fat, and BIA impedances as potential predictor variables. Ground-truth and predicted avatar anthropometric dimensions were quantified with the same software.
Results
Following exploratory studies, one manifold prediction model was moved forward for presentation that included age, weight, height, and %fat as covariates. Predicted and ground-truth avatars had similar visual appearances; correlations between predicted and ground-truth anthropometric estimates were all high (R2s, 0.75–0.99; all p < 0.001) with non-significant mean differences except for arm circumferences (%D ~ 5%; p < 0.05). Concordance correlation coefficients ranged from 0.80–0.99 and small but significant bias (p < 0.05 − 0.01) was present with Bland-Altman plots in 13 of 20 total anthropometric measurements. The mean waist to hip circumference ratio predicted by manifold regression was non-significantly different from ground-truth scanner measurements.
Conclusions
3D avatars predicted from demographic, physical, and other accessible characteristics can produce body representations with accurate anthropometric dimensions without a 3D scanner. Combining manifold regression algorithms into established body composition methods such as DXA, BIA, and other accessible methods provides new research and clinical opportunities.
A CT-based 3D radiomic signatures combined with clinical airway examinations model for evaluation of mask ventilation in patients undergoing oral and maxillofacial surgeries
Authors: Ren He , Qu Ling , Shi Weiwei , Li Ping , Wang Jiayi
Published: July 2024

Objective
The objective of this study is to develop a model that incorporates clinical measurements with 3D radiomic signatures extracted from CT images of oral and maxillofacial surgery patients to evaluate mask ventilation.
Methods
A prospective cohort trial was conducted to enroll patients scheduled for oral and maxillofacial surgery. After obtaining informed consent, clinical measurements and head and neck CT images were collected. The anesthesiologist who managed the airway graded the mask ventilation, with difficult mask ventilation defined as mask ventilation provided without an oral airway or other adjuvant. For radiomics analysis, 3D airway segmentation was extracted and calculated 3D radiomic signatures and corresponding radiological features. Subsequently, features in the clinical measurements model and radiomic signatures model were determined using the least absolute shrinkage and selection operator (LASSO) classifier. A mixed model was developed that incorporated both radiomic signature features and clinical measurement features.
Results
A total of 716 patients were enrolled in the study. The mixed model combined the five 3D radiomic signatures and six clinical measurements, and was found to have the highest predictive accuracy. In the validation group, the mixed group had an area under the curve (AUC) of 0.851, which was higher than the AUC of 0.812 in the clinical measurements model and 0.827 in the radiomic signatures model.
Conclusions
This study developed a mixed model that combines 3D radiomic signatures and clinical measurements. Its application in clinical practice can assist in identifying patients at risk of experiencing difficult mask ventilation during oral and maxillofacial surgeries.
Dissecting Secondary Immunodeficiency: Identification of Primary Immunodeficiency within B-Cell Lymphoproliferative Disorders
Authors: Palacios-Ortega María , Guerra-Galán Teresa , Jiménez-Huete Adolfo , García-Aznar José María , Pérez-Guzmán Marc , Mansilla-Ruiz Maria Dolores , Mendiola Ángela Villegas , López Cristina Pérez , Hornero Elsa Mayol , Rodriguez Alejandro Peixoto , Cortijo Ascensión Peña , Polo Marta , Morales Marta Mateo , Mandly Eduardo Anguita , Cárdenas Mª Cruz , Carrero Alejandra , García Carlos Jiménez , Bolaños Estefanía , Íñigo Belén , Medina Fiorella , Fuente-Muñoz Eduardo de la , Ochoa-Grullón Juliana , García-Solís Blanca , García-Carmona Yolanda , Fernández-Arquero Miguel , Benavente-Cuesta Celina , Diego Rebeca Pérez , Rider Nicholas , Sánchez-Ramón Silvia
Published: July 2024

Distinguishing between primary (PID) and secondary (SID) immunodeficiencies, particularly in relation to hematological B-cell lymphoproliferative disorders (B-CLPD), poses a major clinical challenge. We aimed to analyze and define the clinical and laboratory variables in SID to B-CLPD, identifying overlaps with late-onset PIDs, which could potentially improve diagnostic precision and prognostic assessment. We studied 37 clinical/laboratory variables in 151 patients with SID to B-CLPD. Patients were classified as “Suspected PID Group” when having recurrent-severe infections prior to the B-CLPD and/or hypogammaglobulinemia according to ESID criteria for PID. Bivariate association analyses showed significant statistical differences between “Suspected PID”- and “SID”-groups in 10 out of 37 variables analyzed, with “Suspected PID” showing higher frequencies of childhood recurrent-severe infections, family history of B-CLPD, significantly lower serum Free Light Chain (sFLC) and immunoglobulin concentrations, and lower total leukocyte, and switch-memory B-cell counts at baseline. Rpart machine learning algorithm was performed to potentially create a model to differenciate both groups. The model developed a decision tree with two major variables in order of relevance: sum κ + λ and history of severe-recurrent infections in childhood, with high sensitivity 89.5%, specificity 100%, and accuracy 91.8% for PID prediction. Identifying significant clinical and immunological variables can aid in the difficult task of recognizing late-onset PIDs among patients with SID to B-CLPD, emphasizing the value of a comprehensive immunological evaluation. The differences between “Suspected PID” and SID groups, highlights the need of early, tailored diagnostic and treatment strategies for personalized patient management and follow up.
Exploring Invasive Traits: Symphyotrichum squamatum versus Symphyotrichum ciliatum in Romania – An ESDM, Reproductive, and Morpho-Structural Approach
Authors: Cîșlariu Alina Georgiana , Dumitrașcu Mioara , Mihai Daniela Clara , Andronache Marius Nicu , Camen-Comănescu Petronela , Nagodă Eugenia , Sârbu Anca
Published: July 2024

Symphyotrichum squamatum (Spreng.) G.L.Nesom, a South American species, was first introduced to Europe in 1905 and has since become invasive in numerous countries. In Romania, it was first documented in Bucharest in 2015 and it has since spread to various urban and peri-urban areas. This study aims to assess the invasive potential of Symphyotrichum squamatum in Romania through a comprehensive approach that includes modeling the species’ potential distribution, evaluating its reproductive success, and analyzing morpho-structural characteristics of its vegetative body. Additionally, we compared the species’ traits with those of another alien species from the same genus, Symphyotrichum ciliatum (Ledeb.) G.L.Nesom, which is currently invasive in Romania, thereby demonstrating its potential for invasiveness. The potential distribution of both Symphyotrichum squamatum and Symphyotrichum ciliatum in Romania was modeled using the R software, with the SSDM package and environmental variables corresponding to the species’ ecological preferences, such as climatic variables, local topographic, pedological factors, and anthropogenic impact. Reproductive success of Symphyotrichum squamatum was assessed through germination tests on individuals from different populations. The modeling results suggested that Symphyotrichum squamatum’ distribution in Romania is largely influenced by the anthropogenic impact index and soil classes. Furthermore, the high percentages obtained from germination tests, along with the structural characteristics which highlighted its ability to adapt to a wide range of environmental conditions, indicate that Symphyotrichum squamatum is a species with expansion potential if it establishes under favorable environmental conditions, primarily represented by wetlands along watercourses and marshy areas.
Effect of station selection on VLBI-to-GNSS orientation transfer using space-ties onboard a next generation Galileo satellite
Authors: Sert Hakan , Hugentobler Urs , Karatekin Özgür , Dehant Veronique
Published: July 2024

Each geodetic technique realizes its specific reference frame and traditionally these independent frames are linked through the local-ties at collocation sites. Space ties onboard of a spacecraft can be also used to connect different frames as another complementary approach. Observations of a broadband VLBI transmitter (VT) onboard Galileo satellite by a VLBI ground station network can create such a link between GNSS and VLBI reference frames. In this study, we simulate observations of a VT onboard Galileo satellites and investigate the effect of VT observing network/station selection on the rotation transformation parameters between the VLBI and GNSS frames. We find that the rotation transformation uncertainties can be inflated by more than 25% when a geometrically critical station is removed from the network of 21 ground stations. We additionally analyze various VLBI networks consisting of 8-9 ground stations dedicated to IVS R1/R4 sessions. We report that by adding individual stations into some networks uncertainties are reduced by up to a factor of three in daily solutions and up to 50% for all orientation components when combining solutions covering a Galileo repeat cycle of ten days.
Karadeniz Bölgesi’nde Kurak ve Nemli Dönemlerin SPI Yöntemi Kullanılarak Belirlenmesi
Authors: Deniz Öztürk Yasemin , Ünlü Ramazan
Published: July 2024

Karadeniz bölgesi Türkiye’nin en çok yağış alan bölgesidir. Ancak Karadeniz Bölgesi’nde yağışlar hem yıllar arasında hem de bölge içerisinde önemli farklılıklara sahiptir. Bu durum bölgede kuraklıkların yaşanabilmesine ve kurak-nemli dönemlerin birbirini takip etmesine neden olmaktadır. Bu çalışmada yıllık ve 12 aylık SPI değerlerine göre Karadeniz bölgesinde yaşanan kurak ve nemli dönemlerin belirlenmesi amaçlanmıştır. Bölge genelinden seçilen 26 istasyonun 1960-2020 yılları arasındaki ortalama yağış verilerine göre standardize yağış indeksi (SPI) değerleri hesaplanmıştır. Tespit edilen kurak ve nemli dönemlerin eğilimleri Mann-Kendall trend analizi kullanılarak tespit edilmiştir. Ayrıca ısı haritası kullanılarak Karadeniz Bölgesi kıyı ve iç kesimleri olarak ayrılıp kurak ve nemli dönemleri saptanmıştır. Analiz sonuçlarına göre 1966, 1969, 1974-1977, 1984-1986, 1993-1994, 2006-2007 ve 2019-2020 yıllarının normalden daha az yağış aldığı ve birçok istasyonun kuraklığı şiddetli şekilde olduğu saptanmıştır. 1967, 1988, 1996-1997, 1999, 2009 ve 2016 yıllarının ise normalden fazla yağış aldığını yani nemli karakterde olduğunu göstermektedir. Mann-Kendall trend analiz sonuçlarına göre Batı Karadeniz Bölgesinin kıyı kesimlerinde azalma eğilimde olduğu saptanmamıştır. Fakat azalışta anlamlılık bulunamamıştır. Orta ve Doğu Karadeniz bölgesinde ise artış eğilimi göstermekle birlikte bu eğilim bazı istasyonlarda anlamlı bulunmuştur. Bölgenin yer şekilleri dolayısıyla genel bir kurak ve genel bir nemli dönem olmadığı, doğu-batı doğrultusu ve kıyı-iç kesimlerde kurak ve nemli dönemlerin farklılık gösterdiği saptanmıştır.
Ações de Ensino/Aprendizagem de Restaurações Indiretas por meio de Resina Direta Tratada Termicamente - RDTT: Relato de Experiência
Authors: Santana Ivone Lima , Raposo Carolina Carramilo , Franco Marcela Mayana Pereira
Published: July 2024

Considerando o atendimento odontológico aos pacientes de baixa renda e o aprimoramento dos materiais restauradores, é possível confeccionar restaurações indiretas utilizando resinas compostas diretas. Relata-se a experiência de técnica restauradora, para reabilitar dentes extensamente destruídos, como estratégia de ensino em clínica-escola, e criação de protocolo de atendimento. A técnica, criada em doutorado, aprimorada em projeto de pesquisa clínica e projeto de extensão, atualmente é a principal ferramenta no processo ensino/aprendizagem de restaurações indiretas em um curso de Odontologia de uma Universidade Federal no Nordeste brasileiro. Como estratégias de ensino, são utilizadas aulas expositivas dialogadas, aprendizagem baseada em problemas, sala de aula invertida, protocolo da técnica e treinamento prático em laboratório além de portfólio do caso clínico a ser desenvolvido. Na fase de pesquisa clínica houve treinamento técnico e prático em laboratório antes do atendimento. Após 3 anos, o atendimento de crianças e adolescentes de baixa renda da referida pesquisa foi encerrado e a técnica começou a ser praticada em disciplina curricular na Universidade, onde o planejamento dos casos clínicos são delineados em portfólio elaborado pelo aluno para ser executado na clínica-escola. Ao longo do atendimento, todas as etapas são registradas e acrescidas ao portfólio ilustrado com fotos. Uma importante etapa é a utilização da metodologia ativa Simulação do preparo em gesso, que garante maior segurança ao aluno para executar o preparo planejado e, quando autorizado, preparado em boca. Isso permite concluir que a técnica insere o aluno como sujeito da aprendizagem e o professor como facilitador e mediador do processo.
A rare variant of primary immunodeficiency with a STAT1 GOF defect in the practice of a pulmonologist
Authors: Mizernitskiy Yu. L. , Zorina I. E. , Shudueva A. R. , Bogdanova D. V. , Yukhacheva D. V. , Fadeeva M. S. , Pershin D. E. , Rodina Yu. A. , Shcherbina A. Yu.
Published: July 2024

Chronic mucocutaneous candidiasis is a complication occurring in patients with congenital immune disorders, characterized by recurrent infections of the skin, nails, and mucous membranes caused by C. albicans. The STAT1 gain of function (GOF) defect is a primary immunodeficiency condition resulting from heterozygous gain of function mutations in the STAT1 gene. STAT1 is a regulatory transcription factor and a key component of the JAK-STAT pathway mediating interferon-α/β/γ signaling. GOF mutations in the STAT1 gene lead to hyperphosphorylation of the protein of the same name and increased signaling along the JAK-STAT pathway, which also leads to impaired development of type 17 T helper cells (Th17). This disease most often debuts in childhood, and clinically, it is characterized by chronic mucocutaneous candidiasis, multiorgan autoimmune complications and an increased risk of infectious complications. The article describes the clinical observation of a girl with a rare variant of primary immunodeficiency STAT1 GOF.
Pathogenetic aspects of the development of autism spectrum disorders
Authors: Stupak V. P. , Keshishyan E. S. , Garina S. V.
Published: July 2024

There is currently an increase in the number of patients diagnosed with autism spectrum disorders due to the broad interpretation of the criteria for this diagnosis and an actual increase in the number of children with impaired communication and behavioral functions. There are different in their cause, but clinically similar conditions that are attributed to this group. However, the difference in pathogenetic causes may require different approaches to treatment — selection of pharmacological and pedagogical methods of therapy and rehabilitation of these clinical conditions.

In this article, we plan to discuss possible causes of idiopathic (primary) autism spectrum disorders complex, i.e., when there is no indication that the child has conditions or diseases that may lead to the autism spectrum disorders symptom complex (syndromal autism): perinatal disorders, microanomalies of brain structures, sluggish infections (e.g., CMV infection with smoldering encephalitis), and autoimmune brain damage, chromosomal and genetic diseases with an identified gene with pathogenic significance. When discussing autism spectrum disorders or autism without the above conditions, a genetic model is also assumed, but with the inclusion of a large number of candidate genes, without specifying a clear contribution of each gene to pathogenicity.

Numerous studies show that the mechanism of these disorders in autochthonous disease is related to the disruption of synaptic transmission, changes in the ontogenesis of the nervous system in the context of combinations of genetic disorders, as well as the resulting mechanisms of autoinflammatory changes in the structures of the central nervous system. Changes in the permeability of the hematoencephalic barrier, inflammation and disturbance of the glymphatic system are also considered as probable mechanisms of autism spectrum disorders pathophysiology. As a result of impaired synaptogenesis, differentiation and neurogenesis, the resulting excitotoxicity of neurotransmitters and their metabolites, reliably contribute to the formation of the maintenance of this process.
Prevalence and risk factors for dilatation of sinus of Valsalva in boys with X-linked Alport syndrome
Authors: Aksenova M. E. , Tutelman K. M. , Anikalchuk L. L.
Published: July 2024

X-linked Alport syndrome is multisystem disease caused by mutation in COL4A5. Aortic dilatation described in X-linked Alport syndrome is considered a specific manifestation of the disease.

Purpose. To define prevalence and risk factors for aortic dilatation in boys with X-linked Alport syndrome.

Methods. Retrospective cross-section single center study included boys with X-linked Alport syndrome (n=67, age 10.2±4.6), comparison group consisted of boys with congenital urinary tract abnormalities (n=20, age12.2±4.8). All patients underwent on clinical-laboratory examination and echocardiography. Aorta was measured in the parasternal long-axis view at level of the sinus of Valsalva, aortic dilatation was determined by z-score >2 for BSA.

Results. The prevalence of sinus of Valsalva dilatation did not differ between two groups (0.1 vs 0.15; p=0.47). The sinus of Valsalva dilatation was associated with body mass index (p=0.019), left ventricular diastolic diameter (p=0.01) and left ventricular mass (p=0.01) in children with congenital urinary tract abnormalities, with body mass index (p=0.02) and left ventricular diastolic diameter (p=0.03) in boys with Alport syndrome. No statistically significant effect of blood pressure level, proteinuria, eGFR and type of COL4A5 mutation on aortic dilatation has been demonstrated.

Conclusion. The prevalence of aortic dilatation in boys with X-linked Alport syndrome is higher than in general population, but comparable to children with congenital urinary tract abnormalities. The body mass index and left ventricular diastolic diameter were associated with aortic dilatation in Alport syndrome males. We did not show the relationship between blood pressure load, proteinuria, eGFR and aortic dilatation. Study limitations: small sample size, prevalence of young patients with chronic kidney diseases stage 1–2 and missense mutations in the COL4A5 gene.
Course and outcome of pregnancy in women with Alport syndrome
Authors: Aksenova M. Е. , Dlin V. V.
Published: July 2024

Pregnancy in patients with CKD is associated with maternal and fetal risks.

Purpose. To evaluate course and outcome of pregnancy in Alport syndrome women.

Material and methods. Single-center retrospective study included 88 women with genetically confirmed disease. The information about clinical data at conception, course of pregnancy, delivery (preterm delivery <37 gestation weeks; early preterm delivery <34 gestation weeks), fetus characteristics (intrauterine fetal growth restriction: height <2 z-score for gestation age; small for gestation age: weight <2 z-score) were obtained from medical charts and a cross-sectional survey of women.

Results. Information about 117 term pregnancies (2 — in 26, 3 — in 3 patients) was obtained. The 1/3 of women (q=0.37) had complications of pregnancy: proteinuria (q=0.23), blood hypertension (q=0.1), threat of miscarriage (q=0.21). Preterm and early preterm delivery were seen in 1/4 of women (q=0.26), including early preterm in 3% of cases. Intrauterine fetal growth restriction and small for gestation age were recorded in 9% and 11% neonates, respectively; 7% of babies required a neonatal intensive care unit stay. Proteinuria was the risk factor for preeclampsia (OR=42.35 3±1.1; p<0.001), preterm delivery (OR=11.8±0.5; p<0.001), intrauterine fetal growth restriction (OR=12.2±0.7; p<0.001), small for gestation age (OR=7.2±0.6; p<0.001).

Conclusion. The risk of preeclampsia and fetal growth restriction in women with Alport syndrome and normal kidney function appears comparable to that in the general population. But the disease should be considered as a potential risk factor for preterm delivery. Proteinuria is associated with unfavorable pregnancy and fetal outcome in Alport syndrome.
Clinical variability of the neonatal form of Marfan syndrome in patients with FBN1 gene mutations
Authors: Gritsevskaya D. Yu. , Kuramagomedova R. G. , Vasiliev E. V. , Shkolnikova M. A. , Voinova V. Yu.
Published: July 2024

Neonatal Marfan syndrome (ORPHA:284979) is a severe form of the syndrome that manifests in infancy and rapidly progresses in childhood. The causative variant of the disease is most often localized in exons 24–32 of the FBN1 gene, in the so-called “neonatal region.” The range of clinical manifestations and their severity depend on the type of mutation, its location and the influence of genetic modifiers. Four clinical cases of the neonatal form of Marfan syndrome are presented. Two patients with the same missense mutations and different clinical presentations, a milder patient with a splice site mutation leading to protein shortening, and a girl with severe skeletal damage with deletion of exons 25–29. The purpose of this publication is to analyze the genotype-phenotype correlation of neonatal Marfan syndrome patients with mutations in exons 24–32 of the FBN1 gene.
Sudden cardiac death in children with hypertrophic cardiomyopathy: approaches to prevention
Authors: Leontyeva I. V.
Published: July 2024

Hypertrophic cardiomyopathy is the leading cause of sudden cardiac death in children and adolescents, which in most cases is caused by life-threatening arrhythmias. The article highlights the main risk factors and problems of preventing sudden cardiac death in children with hypertrophic cardiomyopathy. The modern problems of stratification of the risk of sudden cardiac death in children based on the assessment of risk factors and multifactorial mathematical models of risk are considered. The clinical, functional and genetic markers of the risk of sudden death in children in comparison with the adult population are considered. Indications for cardioverter defibrillator implantation for the prevention of sudden death are presented and its effectiveness is evaluated.
TUVA TÜRKÇESİNDE KESİN DIŞILIK İŞARETLEYİCİLERİ: OLASILIK KİPLİĞİ
Authors: Sarıkaya Aksoy Tuğba
Published: July 2024

Kiplik (modality), konuşurun bir önermeyle ilgili yaklaşım ve tutumunu yansıtan dil ile ilgili işaretleyicilerdir ve genellikle önerme ve olay kipliği olmak üzere ikiye ayrılır. Olasılık kipliği de daha çok önerme kipliğinin bir alt başlığı olan bilgi kipliği içinde konumlanmıştır. Olasılık kipliğinde konuşurun önermeyle ilgili tahmin, muhtemellik, varsayım, tereddüt, şüphe, kararsızlık, kesinlik ve kesin dışılık gibi kipsel alanlarıyla ilgili farklı olasılık dereceleri ortaya çıkar. Bu çalışmada Tuva Türkçesinde olasılık bildiren işaretleyiciler Rubin’in (2010) kesinlik derecelendirmesi tablosu temel alınarak incelenmiştir. Bu doğrultuda Tuva Türkçesi üzerine yazılmış gramerler, ders kitapları, sözlükler ve edebî türlerden taranan örneklem neticesinde olasılık kipliğinin biçimsel, biçim-sözdizimsel, sözlüksel ve sözdizimsel işaretleyicilerden oluştuğu tespit edilmiştir. Her bir işaretleyici ile ilgili verilen örnekten sonra önermenin hangi olasılık derecelendirmesi (kesinlik-yüksek olasılık-orta olasılık-düşük olasılık-kesin dışılık) içinde değerlendirildiği belirtilmiştir. Bu çalışma, Tuva Türkçesinde olasılık kipliği işaretleyicilerini belirlemek ve alandaki eksikliği gidermek üzere hazırlanmıştır.
influence of transformational leadership style and work motivation on the performance of PT Tara Outsourcing Employees in PT Bukit Asam Maintenance Work Unit Kertapati Dock Unit
Authors: Oktariadi Satria , Widiyanti Marlina , Yusuf Muhammad , Hanafi Agustina
Published: July 2024

This study was conducted to determine the effect of transformational leadership style and work motivation on the performance of outsourced employees of PT Tara in the maintenance work unit of PT Bukit Asam Kertapati Dock Unit. The population and sample in this study were all outsourced employees of PT Tara in the maintenance work unit of PT Bukit Asam Kertapati Dock Unit in 2023-2024 as many as 33 employees using the census method. The results of multiple linear regression analysis show that transformational leadership style and work motivation have a positive and significant effect on employee performance. Transformational leadership style, it is expected that the role of superiors can provide opportunities for employees to give opinions. Work motivation, it is expected that superiors can provide fair input to employees so that fellow employees can establish good relationships.
Data Integrity Issues With Web-Based Studies: An Institutional Example of a Widespread Challenge
Authors: French Blandine , Babbage Camilla , Bird Katherine , Marsh Lauren , Pelton Mirabel , Patel Shireen , Cassidy Sarah , Rennick-Egglestone Stefan
Published: July 2024

This paper reports on the growing issues experienced when conducting web-based–based research. Nongenuine participants, repeat responders, and misrepresentation are common issues in health research posing significant challenges to data integrity. A summary of existing data on the topic and the different impacts on studies is presented. Seven case studies experienced by different teams within our institutions are then reported, primarily focused on mental health research. Finally, strategies to combat these challenges are presented, including protocol development, transparent recruitment practices, and continuous data monitoring. These strategies and challenges impact the entire research cycle and need to be considered prior to, during, and post data collection. With a lack of current clear guidelines on this topic, this report attempts to highlight considerations to be taken to minimize the impact of such challenges on researchers, studies, and wider research. Researchers conducting web-based research must put mitigating strategies in place, and reporting on mitigation efforts should be mandatory in grant applications and publications to uphold the credibility of web-based research.
Patient Perspectives on AI for Mental Health Care: Cross-Sectional Survey Study
Authors: Benda Natalie , Desai Pooja , Reza Zayan , Zheng Anna , Kumar Shiveen , Harkins Sarah , Hermann Alison , Zhang Yiye , Joly Rochelle , Kim Jessica , Pathak Jyotishman , Reading Turchioe Meghan
Published: July 2024

Background

The application of artificial intelligence (AI) to health and health care is rapidly increasing. Several studies have assessed the attitudes of health professionals, but far fewer studies have explored the perspectives of patients or the general public. Studies investigating patient perspectives have focused on somatic issues, including those related to radiology, perinatal health, and general applications. Patient feedback has been elicited in the development of specific mental health care solutions, but broader perspectives toward AI for mental health care have been underexplored.

Objective

This study aims to understand public perceptions regarding potential benefits of AI, concerns about AI, comfort with AI accomplishing various tasks, and values related to AI, all pertaining to mental health care.

Methods

We conducted a 1-time cross-sectional survey with a nationally representative sample of 500 US-based adults. Participants provided structured responses on their perceived benefits, concerns, comfort, and values regarding AI for mental health care. They could also add free-text responses to elaborate on their concerns and values.

Results

A plurality of participants (245/497, 49.3%) believed AI may be beneficial for mental health care, but this perspective differed based on sociodemographic variables (all P<.05). Specifically, Black participants (odds ratio [OR] 1.76, 95% CI 1.03-3.05) and those with lower health literacy (OR 2.16, 95% CI 1.29-3.78) perceived AI to be more beneficial, and women (OR 0.68, 95% CI 0.46-0.99) perceived AI to be less beneficial. Participants endorsed concerns about accuracy, possible unintended consequences such as misdiagnosis, the confidentiality of their information, and the loss of connection with their health professional when AI is used for mental health care. A majority of participants (80.4%, 402/500) valued being able to understand individual factors driving their risk, confidentiality, and autonomy as it pertained to the use of AI for their mental health. When asked who was responsible for the misdiagnosis of mental health conditions using AI, 81.6% (408/500) of participants found the health professional to be responsible. Qualitative results revealed similar concerns related to the accuracy of AI and how its use may impact the confidentiality of patients’ information.

Conclusions

Future work involving the use of AI for mental health care should investigate strategies for conveying the level of AI’s accuracy, factors that drive patients’ mental health risks, and how data are used confidentially so that patients can determine with their health professionals when AI may be beneficial. It will also be important in a mental health care context to ensure the patient–health professional relationship is preserved when AI is used.
Cooperative Virtual Reality Gaming for Anxiety and Pain Reduction in Pediatric Patients and their Caregivers during Painful Medical Procedures: Research Protocol for a Randomized Control Trial (Preprint)
Authors: Liszio Stefan , Bäuerlein Franziska , Hildebrand Jens , van Nahl Carolin , Masuch Maic , Basu Oliver
Published: July 2024

BACKGROUND

The hospital experience is often marked by fear and pain, particularly for children undergoing medical procedures. Sedation is commonly used to alleviate patient anxiety, but it poses additional health risks. Caregivers, usually the parents, also experience emotional distress during the child's hospital stay, which can further exacerbate the child's anxiety and pain. While various interventions exist to ease patient distress, few consider the emotional well-being of caregivers.

OBJECTIVE

This study aims to explore the effectiveness of a cooperative virtual reality (VR) game as a novel non-pharmacological solution to reduce anxiety and pain for both pediatric patients and their caregivers during medical procedures. Specifically, we investigate whether the VR game "Sweet Dive VR" (SDVR), designed for children aged 6-12 years to play with one caregiver, can alleviate anxiety and pain during different types of needle punctures and Kirschner-wire removal.

METHODS

A prospective multicenter randomized clinical trial is conducted. Eligible participants are identified by scanning the hospital information system, and group allocation follows stratified randomization. During the medical procedure, patients in the VR condition play SDVR with a caregiver present, while patients in the control group listen to a recording of gently crashing waves. Data collection will be carried out through self-reports of patients and caregivers using visual analog scales and questionnaires at two measurement time points: pre- and post-intervention. Additionally, observation by the interviewers occurs during the intervention to capture emotional and pain reactions as well as interaction quality between patients and caregivers and smoothness of the procedure flow using a structured observation protocol. The measured variables encompass patient affect and pain, caregiver affect, player experience, patient experience, and the flow of the procedure.

RESULTS

The study results are expected to be published in March 2025.

CONCLUSIONS

Cooperative VR gaming, as exemplified by SDVR, emerges as a promising intervention to address anxiety and pain in pediatric patients while involving caregivers to support the emotional well-being of both parties. Our approach strives to foster positive shared experiences and to maintain trust between children and caregivers during emotionally challenging medical situations.

CLINICALTRIAL

German Clinical Trials Register DRKS00033544 (https://drks.de/search/de/trial/DRKS00033544)
Effectiveness of 3D Box Model Trainers in Laparoscopic Cholecystectomy Training: A Controlled Study (Preprint)
Authors: Cabrera John , Myrick Makenna Lorryn , Klejc Claudia , Patel Sharad Ronak
Published: July 2024

BACKGROUND

Laparoscopic cholecystectomies (LCs) are a common surgical procedure with significant variability in patient anatomy, making effective training crucial. Traditional training methods, such as cadaver and porcine models, have limitations including high costs, low reusability, and ethical concerns. 3D surgical training models have increasingly been used to supplement the surgical training of medical students. This study introduces a protocol to test such a training model for laparoscopic cholecystectomies, focusing on the capacity of the model to enhance surgical skills, confidence, and understanding among surgical residents.

OBJECTIVE

This study aims to develop a protocol for testing the effectiveness of 3D box model trainers in enhancing surgical residents' technical skills and confidence in performing laparoscopic cholecystectomies.

METHODS

First year surgical residents at the University of Florida will be randomly assigned to either an experimental group using 3D box model trainers or a control group with no training. Both groups will complete pre and post-training assessments, including confidence surveys, procedural step ordering, and anatomical structure labeling. Paired sample t-tests (P = .05) will be used for data analysis.

RESULTS

As of July 2024, our 3D model has been completed, and we have received an IRB exempt study status.

CONCLUSIONS

This study presents a protocol for testing a 3D box model trainer for laparoscopic cholecystectomies. Results from this protocol could support the incorporation of 3D box model trainers into surgical training programs, highlighting their potential to improve technical skills, confidence, and understanding of laparoscopic cholecystectomies among surgical residents.
Protocol for a population health and lifestyle survey of a coastal city in South East England: Health Counts 2024 (Preprint)
Authors: Sherriff Nigel S , Gilchrist Kate , Mirandola Massimo , Huber Jorg , Aicken Catherine , Galvin Kathleen , Sawyer Alexandra , Davidson Susannah L , Gray Ciara , Vass Caroline , Knight Louise , Llewellyn Carrie D
Published: July 2024

UNSTRUCTURED

Background
Health and lifestyle population surveys are important in public health to identify trends, provide data to monitor the effectiveness and reach of public health initiatives and policies, and help allocate health resources more equitably. Surveys are a methodologically robust way of examining the inequalities in health outcomes or access to resources across a number of socio-demographic groups in a defined geographic area. This particular public health survey will provide information that cannot be obtained from other sources.

Aim
The aim of the study will be to provide comprehensive public health relevant data from an adult population in a defined geographic coastal area of South East England. We describe the development of the survey prior to commencing data analyses.

Methods
A cross-sectional, non-interventional (observational) health and lifestyle population survey was developed using a mobile first design approach to recruitment, with the content drawing on a previous iteration of the survey in 2012. Previous Health Counts surveys in this region have been conducted approximately every ten years since 1992 to provide data about trends over time. Extensive rounds of consultation and testing took place between October 2023 and February 2024. The final survey comprised of 102 questions structured around 13 contemporary public health issues in the UK.
Survey distribution was by two rounds of text messaging through all General Practices in Brighton and Hove, to all adults registered with a GP, with a mobile phone who had not opted out of communications; advertising across a range of public facing initiatives; and included targeted outreach activities for potentially marginalised groups e.g. in public libraries and community groups. Enrolment took place between 18th March and 28th April 2024.

Results
A total of 26014 eligible people responded. Data analysis has yet to begin, results will be reported later in 2024/2025.

Conclusions
Health Counts 2024 survey data will help researchers identify population level health inequalities which can be used to inform decisions about future prevention initiatives and health and wellbeing services to meet residents’ needs. Findings will be published in peer reviewed journals and will form evidence to support local and regional public health initiatives.
Mobile health-based motivational interviewing to promote SARS-CoV-2 vaccination in rural adults: Protocol for a pilot randomized controlled trial (Preprint)
Authors: Braun Ashlea , Corcoran Sarah , Tu Doan Khue , Jernigan Cameron , Moriasi Cate , Businelle Michael , Bui Thanh
Published: July 2024

BACKGROUND

Despite documented efficacy, the public health impact of vaccinations is severely limited by misperceptions, hesitancy, and poor acceptance. Messaging from healthcare providers has not yet been optimized to overcome these barriers and has not been tailored to groups that face health disparities, such as rural Americans. Because vaccines have become controversial, as illustrated by the public response to the SARS-CoV-2 vaccines, traditional approaches that use persuasive education or advice to change perspectives are unlikely to have long-term effects and may even be counterproductive. Alternatively, motivational interviewing (MI) is a conversational approach to address modifiable behavior and its empathic nature can be useful when navigating challenging topics. Although MI has been found to be efficacious for improving vaccination rates among children and adolescents, it is unknown whether MI can reduce vaccine hesitancy and health disparities among underserved rural adults. Further, the ideal mode of delivery for MI is unknown, especially “dose,” “intensity,” and integration with mobile health (mHealth). Therefore, it is essential to investigate the efficacy of MI in promoting vaccine uptake in rural populations to reduce health disparities.

OBJECTIVE

Develop and evaluate the feasibility, acceptability, and preliminary efficacy of our mHealth-based motivational interviewing intervention to promote SARS-CoV-2 vaccination (MOTIVACC).

METHODS

Pilot mixed methods 2-phase study: convening a community advisory panel to understand barriers and facilitators to vaccination and mHealth uptake among adults (phase 1, n=16-20), and a pilot 3-group single-blind randomized controlled trial (RCT) for 8 weeks (phase 2, n=60). In the RCT, we recruit adults who have received no previous dose of the COVID-19 vaccine, and randomize them into one of three arms: Standard MI (SMI; n=20), Intensive MI (IMI; n=20), or mHealth-based MOTIVACC (n=20). The primary RCT outcomes are positive change in vaccine hesitancy and intention to obtain the vaccines, measured on Likert scales. The secondary RCT outcome is actual vaccine receipt.

RESULTS

Phase 1 of this study was approved by the ethics committees of both the University of Oklahoma and Oklahoma State University in July, 2022, and was completed June 2022. Phase 2 of this study was approved by the ethics committee at the University of Oklahoma in April, 2024.

CONCLUSIONS

This randomized trial will evaluate the preliminary efficacy of MI for targeting SARS-CoV-2 vaccine hesitancy, as well as compare traditional MI versus mHealth-based MI. This will provide pivotal data on scalable strategies to assist in navigating vaccine hesitancy, including in rural populations.

CLINICALTRIAL

ClinicalTrials.gov NCT05977192
Ensuring Safety: Groundwater Quality and Its Potential Health Effects in the Mgbede Oil Fields Environment of South-South Nigeria. (Preprint)
Authors: Clinton-Ezekwe Ijeoma .Catherine , Raimi Morufu Olalekan , Ezekwe Ifeanyichukwu Clinton , Osu Charles Ikenna , Ordinioha Best
Published: July 2024

BACKGROUND

The quality of groundwater in the Mgbede Oil Fields of South-south Nigeria has become a concern due to potential contamination from industrial activities, particularly gas flaring. Assessing the impact of these activities on groundwater quality is crucial for ensuring the health and safety of local communities and the environment.

OBJECTIVE

This study aimed to evaluate the quality of groundwater in the Mgbede Oil Fields, specifically examining whether the water meets international drinking water standards and identifying any contaminants present due to gas flaring.

METHODS

Purposeful selection of water sampling points was employed to ensure the objectives of the study were met. Groundwater samples were collected from various locations within the Mgbede Oil Fields and analyzed for key quality parameters, including pH, manganese, and turbidity, among others.

RESULTS

The analysis revealed that while most groundwater samples were within acceptable international drinking water limits, there were notable exceptions. pH levels ranged from 5.17 to 6.16, manganese concentrations from 0.001 to 0.136 mg/L, and turbidity levels from 0.00 to 825 mg/L. All sampled locations exhibited traces of manganese and high acidity, indicating particulate pollution likely resulting from gas flaring activities.

CONCLUSIONS

The study concluded that the groundwater quality in the Mgbede Oil Fields is negatively impacted by continuous gas flaring. Contaminants such as manganese and increased acidity levels exceed acceptable international standards, posing potential risks to environmental health. To mitigate these risks, it is recommended that immediate measures be taken to reduce gas flaring and its associated particulate emissions. Regular monitoring of groundwater quality should be implemented to track improvements and ensure compliance with international standards. Additionally, introducing filtration systems or alternative water sources could help protect the health of local communities. This study underscores the significant impact of industrial activities, specifically gas flaring, on groundwater quality in the Mgbede Oil Fields. The findings highlight the urgent need for regulatory measures and continuous monitoring to safeguard environmental health and ensure the availability of safe drinking water.
Висвітлення конференції Американського коледжу кардіології 2024 року
Authors: Authors No
Published: July 2024

No abstract
Менеджмент артеріальної гіпертензії в пацієнток жіночої статі під час особливих станів: вагітність і клімактерій (за рекомендаціями Європейського товариства гіпертензії 2023 року). Методичні рекомендації Київ, 2024
Authors: Authors No
Published: July 2024

No abstract
Экспериментальное исследование и моделирование высокочастотных характеристик Ge-фотодиода для интегральных схем оптических приемников СВЧ-диапазона
Authors: Коколов А.А. , Шеерман Ф.И. , Бабак Л.И. , Конкин Д.А. , Убайчин А.В. , Коряковцев А.С. , Шутов Е.А.
Published: July 2024

Рассмотрена методика измерений высокочастотных характеристик интегрального
Ge-фотодиода с учетом его реального окружения в фотонной (ФИС) или фотонно-электронной (ФЭИС) интегральной схеме и зондовая установка для ее реализации.
Особенностью методики является использование двух источников когерентного оптического излучения с отличающимися длинами волн. Представлены результаты
экспериментального исследования коэффициента оптоэлектронного преобразования Ge-фотодиода в специально разработанной измерительной ФИС, изготовленной по фотонно-электронной SiGe БиКМОП технологии. При длине волны 1 550 нм полоса частот Ge-фотодиода достигает ~30 ГГц, что дает возможность использовать его в составе интегральных оптических приемников со скоростью передачи данных не менее 25 Гбит / с. С использованием электромагнитного моделирования разработана малосигнальная модель Ge-фотодиода, размещенного в ФИС или ФЭИС, которая позволяет провести расчет характеристик монолитно-нтегрированного оптического приемника.