bluuebunny/crossref_metadata_2025 huggingface dataset:
The efficacy outcomes in non-small cell lung cancer patients treated with PD axis inhibitor agents - a population-based study of the Vojvodina region
Authors: Lalić Nensi , Bojović Marko , Bursać Daliborka , Bokan Darijo , Čeriman Krstić Vesna , Kuhajda Ivan , Parapid Biljana , Tomić Sanja , Šipka Aleksandar
Published: July 2024

Background: By 2021, the FDA approved the use of the drugs pembrolizumab and atezolizumab in the first-line treatment of patients with high positivity of programmed death ligand-1 (PD-L1) in locally advanced and metastatic non-small-cell-lung cancer (NSCLC). This approval was the result of statistically significant evidence from international, multicentric clinical studies that all reported increasing progression-free survival (PFS) and overall survival (OS) in these patients.

Methods: In our study, we reported the demographic and clinical characteristics of 79 patients diagnosed with NSCLC with expression of PD-L1 ≥50% from January 2019 to December 2022 at the Institute for Pulmonary Diseases of Vojvodina, who received pembrolizumab therapy as the first-line treatment. Patients were divided according to the histological type of lung cancer as adenocarcinoma (ADC) or squamous cell carcinoma (SCC) of the lung. In 52 of the 79 patients, PFS and in 32 of them overall survival (censored OS) was shown according to the histological type of tumor, the tumor proportion score (TPS) of PDL-1 expression, and the metastatic status within the Tumor Nodes Metastasis (TNM) disease classification. Independent factors of death outcome were shown by multivariable proportional hazard regression analysis.

Results: The study included 79 patients diagnosed with NSCLC with an expression of PD-L1 ≥50%, 50 (63.3%) patients with ADC, and 29 (36.7%) patients with SCC, whose 55 (69.6%) PDL-1 expression was obtained from broncho biopsy (BB). The majority of patients, 49 (62%), had a TPS PD-L1 score of 51%–79%. Median, PFS for adenocarcinoma was 22 months and censored OS was 27 months, while for squamous cell carcinoma, median PFS was 12 months, and censored OS was 21 months. M1b disease stage, which was the most common in patients, had a PFS of 16 months and a censored OS of 18 months.

Conclusion: Pembrolizumab monotherapy in patients with NSCLC in the fourth stage of the disease and with the positivity of the immune checkpoint protein TPS PD-L1 above 50% represents a safe therapy that allows a satisfactory period without disease progression and overall survival with acceptable treatment complications.
Importance of Competitive Dynamics of Strategic Groups: Opportunities and Challenges
Authors: Rosário Albérico , Raimundo Ricardo
Published: July 2024

The competitive dynamics of strategic groups perform a major role in shaping the competitive background of industries. Strategic groups are sets of companies within an industry that follow related strategies or have comparable characteristics, such as product offerings, target markets, or geographic scale. Understanding these dynamics is central for firms to spot opportunities and cope with challenges effectively. This paper reviews the literature on the competitive dynamics of strategic groups. It analyses the theoretical underpinnings of competitive dynamics of strategic groups and discusses the central dimensions of the concept, underscoring the importance of competitive dynamics for strategic groups. Our aim is to sum up these perspectives while providing a clear and integrated overview through a systematic literature review. Such systematic literature review was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method. PRISMA is a framework with evidence-based data designed to help authors carry out a systematic literature review. This systematic literature review sought to consolidate knowledge on this topic. A discussion on the theme is carried out, an ensuing conclusion came up and, finally, avenues for further research are recommended.
Dynamics of Microbial Community Structure and Metabolites during Mulberry Ripening
Authors: Bian Minghong , Fang Yuli , Yuan Tianmeng , Xu Qiang , Xia Yubin , Tang Hongyi , Feng Yiling , Han Baolin
Published: July 2024

In this experiment, we explored the chemical composition and community structure of Mulberry “Wuhedashi” (Morus alba L., hereinafter referred to as WHDS) in different stages and obtained data support for its resource utilisation. Five ripening stages were established: S1, deep-red stage; S2, red with black stage; S3, black with red stage; S4, black stage; S5, overripe stage. The results showed that there were significant differences in the indicators of WHDS in the different stages. Immature WHDS contains high levels of amino acids (2.14 ± 0.15 mg/g), organic acids (43.10 ± 0.75 mg/g), K (3019.52 ± 78.00 mg/L), and Ca (1617.86 ± 24.45 mg/L) and is an important source of amino acid and mineral supplementation for the body. Total phenolic compounds (26.15 ± 0.43 g/L), total flavonoids (2.46 ± 0.03 g/L), total anthocyanins (587.60 ± 7.38 mg/L), the ABTS radical scavenging rate (94.20 ± 2.18%), the DPPH radical scavenging rate (95.13 ± 1.44%), and total flavour compounds (1279.09 ± 33.50 mg/L) peaked at S4, which is the optimal stage for the consumption and processing of WHDS. High-throughput sequencing identified 10 dominant genera, including Bacillus, Brevibacterium, Pseudomonas, and Tatumella. Nesterenkonia was the most highly associated micro-organism. Bacterial genera such as Pantoea and Pseudomonas were significantly positively correlated with esters, enhancing the floral and fruity flavours of wine. The results of the study revealed the characteristics of WHDS in different stages, which can help to target the development of nutritious mulberry derivatives and provide a reference for enhancing the added value of mulberry.
Incidence of Photosensitization in Husbandry Animals: A Meta-Study on the Effects of Feed Diversity and Feed Choice
Authors: Moritz Rieke , Aboling Sabine
Published: July 2024

As this is a meta-study, we examined whether plant species diversity in the feed and the possibility of feed choice would influence the number of cases of photosensitization in farm animals. We evaluated 110 scientific references which described 172 cases of photosensitization worldwide, mainly in husbandry animals between 1926 and 2022. More than 50% of the cases occurred in South America and Australia. Among the animal species, sheep and cattle were statistically overrepresented. A total of 35 organisms were revealed to be phototoxic: 24 herbs, 2 grasses, 7 woody species, and 2 kinds of fungi. Animals developed mainly secondary photosensitization due to fresh feed (71.8%) of normal quality (88.1%), indicating that the phototoxic agents are from liver-toxic plants such as the grass Brachiaria and the herb Froelichia. Horses fell ill chiefly with primary photosensitization due to directly acting phototoxic agents of plant species such as the herbs Medicago and Pastinaca, both in fresh and conserved feed. Goats manage to avoid phototoxic plants under both high and low feed diversity if they still have free choice between plant species. High feed diversity reduced the incidence 2.4-fold, while enabled selection possibility even reduced it 7.5-fold. Since the lack of choice between forage plants was revealed to be the main cause of photosensitization, this knowledge could be used to prevent the disease in livestock.
Gα Solicits OsNYC4 and GW2-WG1-OsbZIP47 Modules to Regulate Grain Size in Rice (Oryza sativa L.)
Authors: Ma Shiwei , Sun Yiqiong , Chen Xuan , Guo Jiayi , Wu Shuhong , Wu Guofeng , Huang Guanpeng , Kabore Manegdebwaoga Arthur Fabrice , Woldegiorgis Samuel Tareke , Ai Yufang , Zhang Lina , Liu Wei , He Huaqin
Published: July 2024

Grain size is one of the critical factors determining rice yield. Previous studies have found the grain-size-regulating function of Gα in rice. However, the regulatory mechanism underlying the development of rice grain mediated by Gα is still unclear. To reveal the functional mechanism of Gα in grain size regulation, a mutant of Gα (Gα-Cas9) was firstly constructed through a CRISPR/Cas9 strategy and was then grown in a greenhouse and field. The results showed that the seed length, plant height, 1000-grain weight, and spike length were significantly decreased in Gα-Cas9 compared to wild-type (WT) Pi-4b. During the grain filling stage, the increase in the grain dry weight of Pi-4b occurred earlier than that of Gα-Cas9. The total starch content and amylose content of matured grains of Pi-4b were higher than those of Gα-Cas9. Secondly, transcriptome sequencing analysis of Gα-Cas9 and Pi-4b during grain filling was performed to elucidate the functional pathways regulated by Gα. In total, 2867 and 4534 differentially expressed genes (DEGs) were discovered at 5 DAF and 10 DAF, and the starch and sucrose metabolism pathway enriched by DEGs was involved in grain size regulation mediated by Gα. Gα regulated the expression of starch-synthesis-related genes during grain filling, and the Gα protein interacted with OsNYC4 to trigger the sugar signaling pathway to promote starch accumulation in grain. Additionally, the GW2-WG1-OsbZIP47 pathway was switched off by Gα to relieve the inhibition of rice grain development. In this study, the results should provide new insights into the G protein signal transduction pathway.
Effects of Drought at Anthesis on Flag Leaf Physiology and Gene Expression in Diverse Wheat (Triticum aestivum L.) Genotypes
Authors: Duvnjak Jurica , Sarcevic Hrvoje , Vukovic Rosemary , Spanic Valentina
Published: July 2024

The current study aimed to quantify the effects of two drought intensities achieved by deprivation of watering for 45 and 65% of the volumetric soil moisture content (VSMC) for 14 days after wheat anthesis to identify physio-biochemical and molecular changes associated with drought tolerance in six genotypes with different drought tolerance. Drought at 65% of VSMC induced a significant decrease in the chlorophyll a content in the drought-sensitive genotype, which indicated a strong loss of photosynthetic reaction centres. Further, in the drought-tolerant genotype, the content of carotenoids, which could play a vital role in resisting water shortage stress, tended to increase. The increased production of malondialdehyde showed that the antioxidant system in the drought-sensitive genotypes was not properly activated. A significant decrease in catalase (CAT; EC 1.11.1.6) was observed at a 45% reduction in VSMC, compared to the control, in the drought-sensitive genotype, and at a reduction in VSMC of 65%, in all medium sensitive genotypes. Further, the drought-tolerant and -medium tolerant genotypes responded to drought with a decline in total glutathione concentrations with the intention to reinforce their defence system. Thereby, dehydroascorbate reductase (DHAR; EC 1.8.5.1), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), and glutathione reductase (GR; EC 1.6.4.2) were critical enzymes involved in the ascorbate–glutathione cycle together with CAT, showing their main role in the detoxification of ROS produced with the reduction in VSMC by 65%. The results of gene expression analysis showed that severe drought increased the levels of the DHN5 and WZY2 genes (that were significantly positively correlated) in the drought-tolerant genotype, whose grain weight, area, and length did not change in maturity. Also, it was seen that DHN5 expression showed a significant positive correlation with grain length and proline content at a 45% reduction in VSMC. The identification of different mechanisms under drought can contribute to the selection of drought-tolerant genotypes.
Cover Crop Species Selection, Seeding Rate, and Termination Timing Impacts on Semi-Arid Cotton Production
Authors: White Clayton David Ray , Burke Joseph Alan , Lewis Katie Lynn , Keeling Will Stewart , DeLaune Paul Bradley , Williams Ryan Blake , Keeling Jack Wayne
Published: July 2024

By improving soil properties, cover crops can reduce wind erosion and sand damage to emerging cotton (Gossypium hirsutum L.) plants. However, on the Texas High Plains, questions regarding cover crop water use and management factors that affect cotton lint yield are common and limit conservation adoption by regional producers. Studies were conducted near Lamesa, TX, USA, in 2017–2020 to evaluate cover crop species selection, seeding rate, and termination timing on cover crop biomass production and cotton yield in conventional and no-tillage systems. The no-till systems included two cover crop species, rye (Secale cereale L.) and wheat (Triticum aestivum L.) and were compared to a conventional tillage system. The cover crops were planted at two seeding rates, 34 and 68 kg ha−1, and each plot was split into two termination timings: optimum, six to eight weeks prior to the planting of cotton, and late, which was two weeks after the optimum termination. Herbage mass was greater in the rye than the wheat cover crop in three of the four years tested, while the 68 kg ha−1 seeding rate was greater than the low seeding rate in only one of four years for both rye and wheat. The later termination timing produced more herbage mass than the optimum in all four years. Treatments did not affect cotton plant populations and had a variable effect on yield. In general, cover crop biomass production did not reduce lint production compared to the conventional system.
Antifungal and Allelopathic Effects of Essential Oil from Calyptranthes concinna DC. Dried Leaves and of Its Major Constituent Elemicin
Authors: Fernandes Cassia C. , Dias Alline L. B. , Santos Jaciel G. dos , da Silva Irles J. M. M. , Miranda Mayker L. D.
Published: July 2024

Essential oils (EOs) are natural products widely used in sustainable agrochemistry, not only because they are biodegradable and safe but also because they are regarded as alternatives to chemical fungicides against fungal species that attack crops. Allelopathy, another field of study, falls within the most recent and sustainable strategies applied to weed suppression to replace synthetic herbicides. Therefore, this study reports the chemical composition and allelopathic and antifungal effects of the EOs extracted from Calyptranthes concinna dried leaves (Cc-EO) and its pure major constituent elemicin. Their antifungal activities were evaluated by the disk diffusion method (DDM) at doses between 0.05 mg/mL and 0.4 mg/mL of Cc-EO and elemicin. The allelopathic effect was evaluated by studying the inhibition of germination and the growth of Lactuca sativa seeds. The chemical composition of Cc-EO was determined by GC-MS and GC-FID analyses. The major constituents of Cc-EO were elemicin (60.5%), α-cadinol (9.0%) and caryophyllene oxide (8.3%). Cc-EO and elemicin were assayed in vitro against 17 fungi of agronomic interest (Aspergillus niger, A. flavus, A. nomius, Penicillium digitatum, P. expansum, Sclerotinia sclerotiorum, S. rolfsii, S. minor, Fusarium graminearum, Myrothecium verrucaria, Corynespora cassiicola, Erwinia psidii, Colletotrichum musae, Alternaria carthami, Rhizoctonia solani, Rhizopus stolonifer and Macrophomina phaseolina). The concentration of Cc-EO (0.4 mg/mL) inhibited 100% of the mycelium growth of seven strains, equal to the fungicide fluazinam, which was used as a positive control. Elemicin showed antifungal activity against all fungi at all concentrations under investigation (above 50%). A strong allelopathic effect was recorded for Cc-EO and elemicin at the dose of 0.28 mg/mL, with the almost total inhibition of germination. This study revealed, for the first time, the strong and remarkable fungicidal and allelopathic effects of Cc-EO and elemicin, an important finding for the agrochemical field.
Deficit Irrigation as an Effective Way to Increase Potato Water Use Efficiency in Northern China: A Meta-Analysis
Authors: Niu Yining , Zhang Ke , Khan Khuram Shehzad , Fudjoe Setor Kwami , Li Lingling , Wang Linlin , Luo Zhuzhu
Published: July 2024

Water scarcity poses a significant threat to the sustainable production of crops in Northern China. Despite this, the effect of water management practices, such as deficit irrigation, on the yield and WUE of potatoes has been rarely explored. Based on the meta-analysis of field experiment data, this study evaluated the influence of deficit irrigation on potato yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) under variable soil types, water-saving ratio, irrigation methods, soil organic carbon (SOC) content, and fertilizer rate in Northern China. Here, we determined that potato WUE and IWUE were significantly increased by 10.0 and 31.6%, respectively, under deficit irrigation, while ET was significantly decreased by 26.3% compared to full irrigation. Conclusively, deficit irrigation significantly reduced potato yields by 16.4% compared to full irrigation. Furthermore, SOC content played a vital role in improving the WUE and alleviating potato yield losses under deficit irrigation. Our study suggested that maximum WUE with lower potato yield losses under deficit irrigation can be achieved in the Central Plains region of China or in yellow loam soil, brown soil, and meadow soil under alternate root-zone irrigation when the water-saving ratio was less than 45% and fertilizer application rates were 300 kg N ha−1, >240 kg P2O5 ha−1, and 181–300 kg K2O ha−1. Overall, these findings highlight the need for a comprehensive understanding of various agricultural management practices and local environmental conditions to optimize the benefits of deficit irrigation in potato-growing regions across Northern China.
A 16S RNA Analysis of Yangzhou Geese with Varying Body Weights: Gut Microbial Difference and Its Correlation with Body Weight Parameters
Authors: Xu Xinlei , Fan Suyu , Wu Hao , Li Haoyu , Shan Xiaoyu , Wang Mingfeng , Zhang Yang , Xu Qi , Chen Guohong
Published: July 2024

China is a major goose-raising country, and the geese industry plays a significant role in animal husbandry. Therefore, goose growth performance (body weight) is a critical topic. Goose gut microbiota influences weight gain by regulating its energy metabolism and digestion. Additionally, the impact of cecal microbial community structure on goose growth and development, energy metabolism, and immunity has been examined. However, most studies have used different additives or feeds as variables. Improving the understanding of the dynamic changes in gut microbial communities in geese of different body weights during their growth and development and their correlation with the host’s body weight is necessary. In this study, the cecal microbiota of healthy Yangzhou geese with large (L) and small (S) body weights, all at the same age (70 days old) and under the same feeding conditions, were sequenced using 16S rRNA. The sequencing results were annotated using QIIME2 (classify-sklearn algorithm) software, and the linkET package was used to explore the correlation between intestinal microorganisms and the body weight of the Yangzhou goose (Spearman). At the phylum level, the Firmicutes/Bacteroidetes ratio in the large body weight group was approximately 20% higher than that in the small body weight group, with Bacteroidetes and Firmicutes exhibiting a highly significant negative correlation. At the genus level, Bacteroides constituted the most abundant microbial group in both groups, although the Prevotellaceae_Ga6A1_group exhibited a higher abundance in the large than the small weight group. Spearman correlation analysis and the linkET package were used to analyze the correlation between cecal microflora and production performance indicators that showed significant differences between the two groups and showed that birth weight was significantly positively correlated with Deferribacterota at the phylum level. At the genus level, leg and chest muscle weights exhibited significant positive correlations with Prevotellace-ae_Ga6A1_group, suggesting its critical role in promoting the growth and development of goose leg and chest muscles. A significant negative correlation was observed between [Ruminococ-cus]_torque and Prevotellaceae_Ga6A1_group. These findings offer a crucial theoretical foundation for the study of gastrointestinal microorganisms and provide insights into the development and formulation of poultry probiotics.
Detection and Phenotypic Antimicrobial Susceptibility of Salmonella enterica Serotypes in Dairy Cattle Farms in the Po Valley, Northern Italy
Authors: Parolini Francesca , Ventura Giordano , Rosignoli Carlo , Rota Nodari Sara , D’incau Mario , Marocchi Leonardo , Santucci Giovanni , Boldini Massimo , Gradassi Matteo
Published: July 2024

The presence of Salmonella spp. in dairy cattle farms poses a major risk to animal health and welfare. This study focused on Salmonella detection in dairy farms located in the Cremona and Mantua provinces (northern Italy) in samples collected and submitted to laboratories in 2021–2022. A total of 2710 samples from different sources, including calf carcasses/organs (n = 128), rectal swabs (n = 1937), feces (n = 390), bulk milk (n = 93), and overshoes/swabs (n = 127) for environmental sampling, were analyzed for the presence of Salmonella spp. and were included in the present study. Our results indicate that Salmonella was most commonly firstly identified from calf carcasses and organs (61.67%) and that the serotypes most frequently detected in dairies were S. Dublin (38.33%), S. Typhimurium (23.33%), and S. Typhimurium monophasic variant (14.17%). The most common pathological findings in calf carcasses were enteritis, hepatosplenomegaly, and pneumonia. The antimicrobial resistance pattern analyzed using the MIC assay of 51 Salmonella isolates revealed the presence of multi-resistant strains, which pose a major risk to public and animal health.
Validation of the Residual Feed Intake Model in Brangus Heifers: Determination of the Optimal Days on Feed Interval to Estimate Dry Matter Intake and Average Daily Gain
Authors: Mahler Lauren E. , Mullenix Mary Kimberly , Brandebourg Terry D. , Kriese-Anderson Lisa A.
Published: July 2024

Brangus cattle are gaining popularity in the Southeast U.S. due to the desirable heat tolerance from their Brahman influence combined with the superior carcass merit aspects of Angus genetics. However, little is known about the optimal evaluation conditions for this hybrid breed when placed on test for Residual Feed Intake (RFI), a heritable measure of feed efficiency that allows improvement in performance without altering carcass traits. To address this, dry matter intake (DMI) was measured on Brangus heifers for 70-d to determine the optimal days on feed required to estimate feed intake and ADG and assess if inclusion of ultrasound measures of carcass merit into the model impact RFI rankings for this breed. The 56-d test period had a regression coefficient of 0.96 (p < 0.0001), R2 = 0.94, rp = 0.97 (p < 0.0001), and rs = 0.97 (p < 0.0001), indicating little change in rank of cattle for DMI compared to a 70-d test. ADG was the limiting factor in determining test duration. Based upon examining only heifers that calved, ultrasound backfat measures should be included in the RFI model to normalize for differences in heifer maturity. Results from this study indicate that a test duration of 56-d is sufficient to accurately estimate DMI in this population. This data indicates on-test duration can be shortened, enhancing the rate of genetic change by reducing cost and increasing the number of animals that can be tested annually.
The Future Is Not Bright: Evaluation of Rat Preferences for Color and Intensity of Light
Authors: Swan Melissa , Horvath Aidan , Pritchett Rebecca K. , Barabas Amanda J. , Hickman Debra , Gaskill Brianna N.
Published: July 2024

Light is a key factor influencing the welfare of laboratory rodents, but little is known about their optimal lighting condition. It i common knowledge that rats prefer dim light, so bright light is mitigated with red-tinted shelters or cages, which alter both the color and intensity of light. Because both aspects are altered, the contribution of each feature to rodent preference is unknown. Further, it is unknown if this preference is influenced by previous experience. We hypothesized that rats would prefer lower light intensity and that their preferences would be influenced by their housing environment. Breeder pairs of rats were randomly separated into four treatments groups: red 200 lux, red 25 lux, clear 200 lux, and clear 25 lux. The breeders’ offspring were tested three times in an apparatus that offered access to each environment, and their preferences were analyzed. Generally, the rats preferred the lower-lux environments and showed no color preference. However, the rats from the clear, 200 lux cages, preferred clear caging and only showed a preference for 25 lux conditions during the second and third preference tests. These results suggest that the light intensity, more than color, should be considered when designing rodent housing and testing facilities.
Effects of Dietary Lonicera flos and Sucutellaria baicalensis Mixed Extracts Supplementation on Reproductive Performance, Umbilical Cord Blood Parameters, Colostrum Ingredients and Immunoglobulin Contents of Late-Pregnant Sows
Authors: Fang Chengkun , Tang Xiaopeng , Zhang Qingtai , Yu Qifang , Deng Shengting , Wu Shusong , Fang Rejun
Published: July 2024

The present study aimed to determine the effects of dietary Lonicera flos and Sucutellaria baicalensis mixed extract (LSE) supplementation during the late-pregnancy period on the reproductive performance, umbilical cord blood hematological parameters, umbilical cord serum biochemical parameters, immune indices, hormone levels, colostrum ingredients, and immunoglobulin contents of sows. A total of 40 hybrid pregnant sows were randomly assigned to the control group (CON; sows fed a basal diet) and LSE group (LSE; sows fed a basal diet supplemented with 500 g/t PE). The results indicated that dietary LSE supplementation significantly increased (p < 0.05) the number of alive and healthy piglets and the litter weight at birth, and significantly increased (p < 0.05) the platelet counts in umbilical cord blood. Dietary LSE supplementation significantly increased (p < 0.05) the levels of prolactin (PRL) and growth hormone (GH), and the content of interleukin 2 (IL-2) in umbilical cord serum. Moreover, immunoglobulin A (IgA) and immunoglobulin M (IgM) in the colostrum were increased with PE supplementation (p < 0.05). In conclusion, dietary LSE supplementation in late-pregnancy sows could improve reproductive performance and colostrum quality, and could also regulate the levels of reproductive hormone in umbilical cord serum.
Immune-Related Adverse Events Associated with Atezolizumab: Insights from Real-World Pharmacovigilance Data
Authors: Frey Connor , Etminan Mahyar
Published: July 2024

The advancement of immuno-oncology has brought about a significant shift in cancer treatment methods, with antibody-based immune checkpoint inhibitors like atezolizumab leading the way in this regard. However, the use of this checkpoint blockade can result in immune-related adverse events due to increased T-cell activity. The full spectrum of these events is not yet completely understood. In this study, the United States FDA Adverse Event Reporting System (FAERS) was utilized to investigate immune-related adverse events linked with the use of atezolizumab. The study identified forty-nine immune-related adverse events that affected multiple organ systems, including cardiovascular, respiratory, hematologic, hepatic, renal, gastrointestinal, neurologic, musculoskeletal, dermatologic, endocrine, and systemic disorders. The strongest signals for relative risk occurred for immune-mediated encephalitis (RR = 93.443), autoimmune myocarditis (RR = 56.641), immune-mediated hepatitis (RR = 49.062), immune-mediated nephritis (RR = 40.947), and autoimmune arthritis (RR = 39.382). Despite the morbidity associated with these adverse events, emerging evidence suggests potential associations with improved survival outcomes. Overall, this report sheds light on the widespread immune-related adverse events that cause significant morbidity and mortality in patients with cancer being treated with atezolizumab and brings attention to them for the clinicians treating these patients.
The Oral Cavity—Another Reservoir of Antimicrobial-Resistant Staphylococcus aureus?
Authors: Chmielewski Marek , Załachowska Oliwia , Komandera Dominika , Albert Adrian , Wierzbowska Maria , Kwapisz Ewa , Katkowska Marta , Gębska Alina , Garbacz Katarzyna
Published: July 2024

Staphylococcus aureus is one of the most common potentially pathogenic bacteria that may asymptomatically colonize many sites of healthy carriers. Non-nasal carriage, especially in the oral cavity, and its role in transmitting antimicrobial-resistant S. aureus strains in the healthcare community, is poorly understood. This study aimed to assess the prevalence and antimicrobial susceptibility of S. aureus in both oral and nasal cavities among preclinical dentistry students. A total of 264 oral and nasal swabs were taken from 132 participants, and all specimens were cultured using standard diagnostic procedures and antimicrobial susceptibility testing (EUCAST). The prevalence of S. aureus exclusively in the nasal (11.4%) or oral (9.1%) cavity was comparable, while concurrent oral and nasal carriage was present in 27.3% of participants. Although antibiotic resistance rates observed in both oral and nasal isolates were similar (ranging from 2.7% to 95.5%), 16.7% of carriers exhibited distinct antibiotic resistance profiles between oral and nasal isolates. Three (2.7%) methicillin-resistant S. aureus (MRSA) were isolated from the mouth and nose but multidrug resistance (27.3%) was more frequent in the oral than in the nasal isolates: 34% and 21.1%, respectively. This study demonstrated that preclinical dentistry students have a similar rate of oral S. aureus carriage as the nasal carriage rate, and that the oral cavity can be colonized by antimicrobial-resistant strains that do not originate from the nose. Consequently, the oral cavity seems to be an unjustly overlooked body site in screening for S. aureus carriage.
Screening Tests for the Interaction of Rubus idaeus and Rubus occidentalis Extracts with Antibiotics against Gram-Positive and Gram-Negative Human Pathogens
Authors: Hałasa Rafał , Mizerska Urszula , Kula Marta , Krauze-Baranowska Mirosława
Published: July 2024

WHO (World Health Organization) reports from recent years warn about the growing number of antibiotic-resistant bacterial strains. Therefore, there is an urgent need to constantly search for new substances effective in the fight against microorganisms. Plants are a rich source of chemical compounds with antibacterial properties. These compounds, classified as secondary metabolites, may act independently or support the action of currently used antibiotics. Due to the large number of metabolites isolated from the plant kingdom and new plant species being studied, there is a need to develop new strategies/techniques or modifications of currently applied methods that can be used to select plant extracts or chemical compounds isolated from them that enter into positive, synergistic interactions with currently used antibiotics. One such method is the dual-disk synergy test (DDST). It involves the diffusion of active compounds in the agar environment and influencing the growth of microorganisms grown on it. The method was used to assess the interaction of extracts from the fruit and shoots of some cultivated varieties of Rubus idaeus and Rubus occidentalis with selected antibiotics. The research was conducted on strains of bacteria pathogenic to humans, including Staphylococcus aureus, Corynebacterium diphtheriae, Escherichia coli, Pseudomonas aeruginosa, Helicobacter pylori, and Candida albicans, showing synergy, antagonism, or lack of interaction of the tested substances—plant extract and antibiotic. As a result, it was found that the diffusion method is useful in screening tests to assess the impact of antibiotic–herbal substance interactions on Gram-positive and Gram-negative microorganisms.
Impact of Intermittent Fasting and Dietary Restriction on Redox State, Energetic Metabolism, and Liver Injury in Common Bile Duct Ligation Model
Authors: Semenovich Dmitry S. , Zorova Ljubava D. , Abramicheva Polina A. , Andrianova Nadezda V. , Elchaninov Andrey V. , Petrukhina Aleksandra S. , Pevzner Irina B. , Manskikh Vasily N. , Zorov Dmitry B. , Plotnikov Egor Y.
Published: July 2024

The aim of this work was to test whether we can treat cholestasis with dietary approaches applied after the onset of the disease. The effects of intermittent fasting and dietary restriction on liver damage caused by common bile duct ligation (BDL) in rats were studied, with particular attention paid to changes in the activity of enzymes of energy metabolism and antioxidant protection. Morphological changes in liver tissue and serum markers of liver damage were assessed in rats with BDL kept for one month on ad libitum diet, intermittent fasting, or 35% dietary restriction. We studied parameters of glucose metabolism (activity of glycolysis and gluconeogenesis enzymes), TCA cycle, and indicators of oxidative stress and redox status of the liver tissue. Dietary restriction resulted in an increase in gluconeogenesis activity, antioxidant capacity, and autophagy activation. When implemented after BDL, none of the dietary restriction protocols reduced the level of oxidative stress, detrimental morphological and biochemical alterations, or the fibrosis progression. Thus, under severe damage and oxidative stress developing in cholestasis, dietary restrictions are not hepatoprotective and can only be used in a pre-treatment mode.
The Effect of Selected Nitric Oxide Synthase Polymorphisms on the Risk of Developing Diabetic Nephropathy
Authors: Król-Kulikowska Magdalena , Banasik Mirosław , Kepinska Marta
Published: July 2024

Background: Nitric oxide synthase (NOS) is an enzyme that catalyzes the formation of nitric oxide (NO), the altered production of which is characteristic of diabetic nephropathy. NOS exists in three isoforms: NOS1, NOS2, and NOS3. Moreover, there are reports about the potential role of NOS3 polymorphisms in the development of diabetes complications. The aim of this study was to assess the role of selected NOS polymorphisms—rs3782218 (NOS1), rs1137933 (NOS2), rs1799983, rs2070744, and rs61722009 (NOS3)—in the risk of developing diabetic nephropathy and in the likelihood of renal replacement therapy. Methods: The studied polymorphisms were analyzed in a group of 232 patients divided into three groups. Four polymorphisms (rs3782218, rs1137933, rs1799983, rs2070744) were genotyped using the PCR-RFLP, while the rs61722009 polymorphism was genotyped using the PCR. Results: The C/C genotype and the C allele of the rs3782218 polymorphism (NOS1) were associated with an increased risk of developing diabetic nephropathy and an increased likelihood of renal replacement therapy. In turn, the G allele of the rs1137933 polymorphism (NOS2) reduces the likelihood of renal replacement therapy. Conclusions: The specific genotypes or alleles of the rs3782218 (NOS1) and rs1137933 (NOS2) polymorphisms seem to be potential risk factors for diabetic nephropathy and renal replacement therapy.
Hearing Loss and Oxidative Stress: A Comprehensive Review
Authors: Maniaci A. , La Via L. , Lechien J. R. , Sangiorgio G. , Iannella G. , Magliulo G. , Pace A. , Mat Q. , Lavalle S. , Lentini M.
Published: July 2024

Hearing loss is a prevalent condition affecting millions of people worldwide. Hearing loss has been linked to oxidative stress as a major factor in its onset and progression. The goal of this thorough analysis is to investigate the connection between oxidative stress and hearing loss, with an emphasis on the underlying mechanisms and possible treatments. The review addressed the many forms of hearing loss, the role of reactive oxygen species (ROS) in causing damage to the cochlea, and the auditory system’s antioxidant defensive mechanisms. The review also goes over the available data that support the use of antioxidants and other methods to lessen hearing loss brought on by oxidative stress. We found that oxidative stress is implicated in multiple types of hearing loss, including age-related, noise-induced, and ototoxic hearing impairment. The cochlea’s unique anatomical and physiological characteristics, such as high metabolic activity and limited blood supply, make it particularly susceptible to oxidative damage. Antioxidant therapies have shown promising results in both animal models and clinical studies for preventing and mitigating hearing loss. Emerging therapeutic approaches, including targeted drug delivery systems and gene therapy, offer new possibilities for addressing oxidative stress in the auditory system. The significance of this review lies in its comprehensive analysis of the intricate relationship between oxidative stress and hearing loss. By synthesizing current knowledge and identifying gaps in understanding, this review provides valuable insights for both researchers and clinicians. It highlights the potential of antioxidant-based interventions and emphasizes the need for further research into personalized treatment strategies. Our findings on oxidative stress mechanisms may also affect clinical practice and future research directions. This review serves as a foundation for developing novel therapeutic approaches and may inform evidence-based strategies for the prevention and treatment of hearing loss, ultimately contributing to improved quality of life for millions affected by this condition worldwide.
Research on High-Precision Dynamic Modeling and Performance Evaluation of Inertially Stabilized Platforms
Authors: Li Baoyu , Xie Xin , Liao Yuwen , Fan Dapeng
Published: July 2024

The complex influence of various disturbances on an inertially stabilized platform (ISP) restricts the further improvement of its servo performance. This article investigates the mapping relationship between internal and external disturbances and servo performance by establishing a high-precision dynamics model of the servo device with simulation and experiment. For internal disturbances, a nonlinear model of friction and backlash is established based on a BP neural network, and the transmission error is reconstructed based on the principle of main order invariance. For external disturbances, the road disturbance torque, changing inertia, and mass imbalance torque are modeled. The quantitative mapping relationship between internal and external disturbances and servo performance is obtained through simulation, in which friction and road disturbance are the largest internal and external factors affecting the servo performance, respectively. These conclusions are verified by load simulation experiments on a certain type of servo device, in which the servo performance is improved by 17% for every 25% reduction of friction torque, and the servo performance is reduced by 12% for every 33% increase of road disturbance torque. The research results provide a reference for servo device selection, performance indicator assignment, and performance prediction of the ISP.
Experimental Investigation on the Dynamic Characteristics of Bubble-in-Chain Near a Vertical Wall
Authors: Cai Runze , Sun Jiao , Chen Wenyi
Published: July 2024

The motion of near-wall bubble-in-chain, which is a crucial aspect of the study of near-wall bubble flows, involves not only the wall effect but also the interactions between bubbles. However, there have been few studies on this topic. In this study, we investigated the motion of near-wall bubble-in-chain using a dual-camera orthogonal shadow method and tracked bubbles using image processing and feature matching techniques. Considering both the wall effect and bubble generation frequency, we discussed the statistical characteristics, motion modes, dynamic characteristics, and energy evolution of bubbles. The results demonstrate that an increase in bubble generation frequency leads to a greater deviation of bubble trajectories from the wall and an increase in trajectory amplitude while weakening the suppression of bubble speed by the wall. Furthermore, changes in both bubble equivalent diameter and drag coefficient reveal how bubble generation frequency affects their shape stability during motion as well as regulation by the wall effect. The drag coefficient decreases with increasing Reynolds number for bubbles; however, an increase in bubble generation frequency broadens its distribution range. Additionally, it is evident that the wall effect significantly impacts drag characteristics for bubbles: uncollided bubbles experience increased drag coefficients with greater distance from the wall while collided bubbles exhibit decreased drag coefficients. In cases of high generation frequency, the conversion of kinetic energy to surface energy during bubble collisions, especially the enhancement of the peak of surface energy, indicates an increase in the bubble’s energy storage capacity and energy conversion efficiency. The findings not only enhance comprehension of behavior exhibited by near-wall bubbles but also offer a novel perspective for regulating near-wall bubble flows in industrial applications.
Numerical and Experimental Demonstration of a Silicon Nitride-Based Ring Resonator Structure for Refractive Index Sensing
Authors: Butt Muhammad A. , Kozłowski Łukasz , Golas Michał , Slowikowski Mateusz , Filipiak Maciej , Juchniewicz Marcin , Bieniek-Kaczorek Aleksandra , Dudek Michał , Piramidowicz Ryszard
Published: July 2024

In optical communication and sensing, silicon nitride (SiN) photonics plays a crucial role. By adeptly guiding and manipulating light on a silicon-based platform, it facilitates the creation of compact and highly efficient photonic devices. This, in turn, propels advancements in high-speed communication systems and enhances the sensitivity of optical sensors. This study presents a comprehensive exploration wherein we both numerically and experimentally display the efficacy of a SiN-based ring resonator designed for refractive index sensing applications. The device’s sensitivity, numerically estimated at approximately 110 nm/RIU, closely aligns with the experimental value of around 112.5 nm/RIU. The RR sensor’s Q factor and limit of detection (LOD) are 1.7154 × 104 and 7.99 × 10−4 RIU, respectively. These congruent results underscore the reliability of the two-dimensional finite element method (2D-FEM) as a valuable tool for accurately predicting and assessing the device’s performance before fabrication.
The Role of the Microalgae–Bacteria Consortium in Biomass Formation and Its Application in Wastewater Treatment Systems: A Comprehensive Review
Authors: Satiro Josivaldo , dos Santos Neto Antonio G. , Marinho Talita , Sales Marcos , Marinho Idayana , Kato Mário T. , Simões Rogério , Albuquerque Antonio , Florencio Lourdinha
Published: July 2024

The optimization of wastewater treatment technologies using biological processes is no longer limited to improving the removal of organic matter and nutrients, as it is possible to reduce area and energy consumption, and recover value-added by-products. In this context, the microalgae–bacteria consortium is an alternative for reducing costs, as microalgae produce the oxygen required by bacteria to oxidize organic matter through photosynthesis. Additionally, it is possible to extract different by-products such as lipids, biofertilizers, biogas, alginate-type exopolymers, and others. Furthermore, bioflocculation occurs naturally through the adhesion of microalgae to the surface of bacterial flocs, without the addition of chemical products. This review discusses the main systems that utilize the microalgae–bacteria consortium, the metabolism of the microalgae–bacteria consortium, and its performance in removing organic matter and nutrients, as well as the effect of operating conditions on the physical properties of the biomass. Among the highlighted systems are sequencing batch and single-batch reactors, high-rate ponds, and continuous flow reactors. Among the systems discussed in this work, the sequential batch reactor configurations found better biomass formation and production of extracellular polymeric substances and the continuous flow reactors showed lower installation and operating costs. From this perspective, the potential for full-scale application of each system can be evaluated once the optimum operating conditions have been defined and the limitations of each system have been understood.
Enhancing Survival Analysis Model Selection through XAI(t) in Healthcare
Authors: Berloco Francesco , Marvulli Pietro Maria , Suglia Vladimiro , Colucci Simona , Pagano Gaetano , Palazzo Lucia , Aliani Maria , Castellana Giorgio , Guido Patrizia , D’Addio Giovanni , Bevilacqua Vitoantonio
Published: July 2024

Artificial intelligence algorithms have become extensively utilized in survival analysis for high-dimensional, multi-source data. However, due to their complexity, these methods often yield poorly interpretable outcomes, posing challenges in the analysis of several conditions. One of these conditions is obstructive sleep apnea, a sleep disorder characterized by the simultaneous occurrence of comorbidities. Survival analysis provides a potential solution for assessing and categorizing the severity of obstructive sleep apnea, aiding personalized treatment strategies. Given the critical role of time in such scenarios and considering limitations in model interpretability, time-dependent explainable artificial intelligence algorithms have been developed in recent years for direct application to basic Machine Learning models, such as Cox regression and survival random forest. Our work aims to enhance model selection in OSA survival analysis using time-dependent XAI for Machine Learning and Deep Learning models. We developed an end-to-end pipeline, training several survival models and selecting the best performers. Our top models—Cox regression, Cox time, and logistic hazard—achieved good performance, with C-index scores of 0.81, 0.78, and 0.77, and Brier scores of 0.10, 0.12, and 0.11 on the test set. We applied SurvSHAP methods to Cox regression and logistic hazard to investigate their behavior. Although the models showed similar performance, our analysis established that the results of the log hazard model were more reliable and useful in clinical practice compared to those of Cox regression in OSA scenarios.
Virtual Streamline Traction: Formation Cooperative Obstacle Avoidance Based on Dynamical Systems
Authors: Liu Yiping , Zhang Jianqiang , Zhang Yuanyuan , Wang Jiarui
Published: July 2024

Formation obstacle avoidance is a critical aspect of cooperation among unmanned surface vehicles (USVs). In practical scenarios involving multiple USVs, managing obstacle avoidance during formation assembly and navigation is essential to ensure the success of cooperative tasks. This study devised a formation cooperative obstacle-avoidance scheme utilizing dynamical systems (DS). The traditional interfered fluid dynamical system (IFDS) applied in two-dimensional planes was enhanced to address local minima issues. Furthermore, robust virtual structure patterns were implemented to effectively decouple velocity vectors. Streamlines were optimized by adjusting velocity amplitudes within specific distance intervals, facilitating precise formation assembly amidst multiple obstacles. Additionally, a novel inter-vehicle disturbance method, distinct from the IFDS, was developed to enhance inter-vehicle collision avoidance. The effectiveness of the proposed method in enabling USV formations to adeptly navigate obstacles while maintaining formation integrity and collision-avoidance capabilities was analyzed theoretically and confirmed through simulation.