bluuebunny/crossref_metadata_2025 huggingface dataset:
The differences of body measurements in captive Sumatran elephant on different captivity management and sex
Authors: WIBOWO DENY SETYO , IBRAHIM ALEK , WIDAYANTI RINI , NURCAHYO WISNU , WAHYU MUHAMMAD , MICHAUX JOHAN R. , WIJAYANTO HERY
Published: August 2021

Abstract. Wibowo DS, Ibrahim A, Widayanti R, Nurcahyo W, Wahyu M, Michaux J, Wijayanto H. 2021. The differences of body measurements in captive Sumatran elephant on different captivity management and sex. Biodiversitas 22: 2619-2624. Sumatran elephants are one of wild animals in Indonesia that currently received critically endangered status. This study aimed to measure the body size of both sexes of Sumatran elephants in the semi-captive (ECCs) and full captive (Zoo) management for conservation purposes. This study was conducted in ECCs in Sumatran island and Zoo on Java island. Body measurements (Chest Girth, Length, Height, Tarsal and Carpal Circumference) were collected from a total of 49 samples of captive Sumatran elephants (25 from EECs and 24 from Zoo). Data were analyzed using a completely randomized factorial design with different management and sex as the factors. The results showed that BL (304.92 vs 311.13) and TC (76.44 vs 77.50) were significantly different (P<0.05) across captivity managements. Sex categories (male and female) showed significant results (P<0.01) on CG (321.71 vs 302.46), BL (303.50 vs 272.49), BH (230.14 vs 208.23) and TC (82.29 vs 74.83). No interaction was identified between captive management and sex on body measurements. In conclusion, there were significant differences in body size of male and female elephants. This result contributed well-researched information to evaluate the conservation management based on body size and for further research on Sumatran elephant conservation.
Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia
Authors: Puspitarini Retno Dyah , AFANDHI AMINUDIN , FERNANDO ITO
Published: August 2021

Abstract. Puspitarini RD, Afandhi A, Fernando I. 2021. Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia. Biodiversitas 22: 2648-2655. The broad mite Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae) has been known to cause a great deal of injury on Jatropha curcas (L.) plantation in Indonesia. Isolation of indigenous fungal entomopathogens from rhizosphere soils, followed by pathogenicity assay was conducted to find an effective isolate for controlling P. latus. Additionally, the potential combination of the selected isolate and soursop (Annona muricata L.) aqueous leaf extract (SLE) was investigated. A total of 24 isolates were obtained by using the insect bait method. Four fungal isolates, namely Paecilomyces sp. 1., Lecanicillium sp., Beauveria sp., and Fusarium sp. 1, showed high conidial viability and were chosen as representatives to assess their pathogenicity against P. latus. Among the tested isolates, Beauveria sp. which had the highest conidial viability among the tested isolates, needed a shorter period to completely kill the tested mites. However, the compatibility test revealed the deleterious effect of SLE on Beauveria sp. SLE at all concentrations showed a very toxic effect on the fungus, therefore the two must be applied separately. Our results provide useful information on the effectiveness of indigenous entomopathogenic fungi Beauveria sp. and aqueous leaf extract of A. muricata as an alternative tool to control the broad mite P. latus on the J. curcas plantation.
Regression model analysis for prediction of body weight from body measurements in female Batur sheep of Banjarnegara District, Indonesia
Authors: Ibrahim Alek , Artama Wayan Tunas , Budisatria I Gede Suparta , Yuniawan Ridwan , Atmoko Bayu Andri , Widayanti Rini
Published: August 2021

Abstract. Ibrahim A, Artama WT, Budisatria IGS, Yuniawan R, Atmoko BA, Widayanti R. 2021. Regression model analysis for prediction of body weight from body measurements in female Batur sheep of Banjarnegara District, Indonesia. Biodiversitas 22: 2723-2730. Bodyweight is an important aspect of livestock management. The present study was undertaken to estimate correlation coefficients between biometric traits and identify best predictor of body weight in female Batur sheep from body measurements. Data on body weight and body measurements (body length: BL, chest girth: CG and withers height: WH) were collected from 73 female Batur sheep in Batur Village, Banjarnegara District, Central Java Province, Indonesia. Batur sheep were grouped into 3 categories based on their age, namely groups <1.5 years, 1.5-2.5 years and >2.5 years. The data were analyzed using simple, multiple, and automatic linear regression methods using the SPSS computer software version 25 platform. The correlation coefficient, coefficient determination, adjusted coefficient determination, residual standard error, Akaike information criterion, Bayesian information criterion, and Akaike information criterion corrected were used to determine the best regression formula for the prediction of BW. The average BW (kg), BL (cm), CG (cm), and WH (cm) of 49.27, 63.11, 91.41, and 56.82, respectively was observed in the present study. The correlation coefficients of 0.433, 0.866, and 0.369 for BW with BL, CG, and WH were observed in the present study. The best prediction of BW using two predictors (BL and GC) was BW =-56.522 + 0.509BL + 0.843CG, followed by using three predictors (BL, CG, and WH) was BW =-57.897+ 0.505BL + 0.839CG + 0.034WH, and using the only one predictor (CG) was BW =-28.443 + 0.905CG. The study revealed that CG and its combination with other linear body measurements can effectively define the body weight in Batur sheep. However, the highest R2 of 0.782 was observed when CG and BL were used as predictors.
Analysis of morphological characteristics and phenetic relationship of ebony (Diospyros spp.) in Indonesia
Authors: Rindyastuti Ridesti , Hapsari Lia , Wibowo Anjar Tri
Published: August 2021

Abstract. Rindyaastuti R, Hapsati L, Wibowo AT. 2021. Analysis of morphological characteristics and phenetic relationship of ebony (Diospyros spp.) in Indonesia. Biodiversitas 22: 2738-2753. Diospyros L. (Ebenaceae) is an economically important genus that covers over 500 plant species. Members of this group are known to produce hard, dark, high-quality timbers known as ebony trees, while other members are known as persimmon trees. There is limited information on the morphological characters and phenetic relationship of this genus from Indonesian archipelago. In this work, we reported the phenetic clustering of 14 species of Indonesian Diospyros, based on plant habit, stem, leaves, and fruit characters, which was analysed using UPGMA and Jaccard similarity index. The phenetic dendrogram divided the 14 species of Diospyros into two main clusters which separating Subgen. Maba and Eudiospyros. Further, subgen. Maba was clustered into a single cluster while subgen. Eudiospyros was divided into 3 sub-clusters as sister groups. The clustering in Eudiospyros was supported by low SI and bootstrap value, demonstrating the high morphological variation of the subgenus. The sectional separation of Diospyros spp. was considered as paraphyletic. In general, our phenetic clustering exhibited suitability and relevancy with previous classification. Fruit size and trichomes are very important in this phenetic clustering, especially to form Eudiospyros subgenus. Fruit trichomes were also special characters related to biogeographical distribution following the Wallace line theory.
Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal
Authors: Sari Siti Lusi Arum , Triyanto Triyanto , Zuprizal Zuprizal , Prijambada Irfan Dwidya
Published: August 2021

Abstract. Sari SLA, Triyanto T, Zuprifal Z, Prijambada ID. 2021. Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal. Biodiversitas 22: 2829-2837. Palm kernel meal (PKM) is potential to be used as feed, but its high fiber content causes PKM meal difficult to be digested by monogastric animals. Ruminants are especially effective in digesting plant fibers because of the presence of microbes in their rumens. Based on those facts, this research was conducted to obtain mannanolytic and cellulolytic bacteria from buffalo rumens (Bubalus babalis, Linnaeus, 1758), which can degrade fibers in PKM. Bacteria were isolated from buffalo rumen by using PKM- isolation media. Screening of hydrolytic activities was done based on clear zone formation on screening media. A total of five bacterial isolates with the highest hydrolytic activities were then assayed quantitatively for their abilities to degrade mannan and cellulose, then identified based on 16S rRNA gene sequences. This research successfully isolated 34 bacterial isolates. The screening result demonstrated that all isolates could hydrolyze mannan, cellulose and polysaccharide in PKM. Isolate BR25 showed the highest hydrolytic ability on PKM and mannan screening media with clear zone diameter/colony diameter ratio (dz/dc ratio) of 2.99 and 3.53, respectively.  Isolate BR31 showed the highest cellulolytic ability with dz/dc ratio value of 2.22. Five isolates with the highest hydrolytic activity, i.e. BR14, BR16, BR23, BR25, and BR30 showed the ability to grow on submerged media which contain locust bean gum (LBG) and microcrystalline cellulose (MCC) respectively, as single carbon source and isolate BR25 showed the highest ability to degrade mannan and cellulose. Based on the gene sequence of 16S rRNA, isolates BR14, BR16, BR23, BR25, and BR30 were identified to be closely related to Exiguobacterium acetylicum, Bacillus cereus, Klebsiella quasipneumoniae, Paenibacillus polymyxa, and Acinetobacter baumannii with 98.57-100% level of similarity.
Ethnobotanical study of medicinal plants used by community in Jambur Labu Village, East Aceh, Indonesia
Authors: ELFRIDA ELFRIDA , TARIGAN NINTA SABRINA , Suwardi Adi Bejo
Published: August 2021

Abstract. Elfrida, Tarigan NS, Suwardi AB. 2021. Ethnobotanical study of medicinal plants used by community in Jambur Labu Village, East Aceh, Indonesia. Biodiversitas 22: 2893-2900. Jambur Labu is a village in East Aceh that is home to various ethnic groups. The community in the village has used various plant species as a traditional medicine to treat diseases and disorders. The aim of this study was to investigate the medicinal plants used by the community in the Jambur Labu Village, Birem Bayeun Sub-district, East Aceh, Indonesia. This study was based on field surveys, plant collection, and interviews with the local people. Interviews were performed with 30 informants selected using the Snowball Sampling technique. A total of 107 medicinal plant species, belonging to 87 families, were documented to be used by the local community in Jambur Labu Village. Leaves were the most used plant part (38%), followed by fruit (22%), whole plant (9%), rhizome (8%), roots (7%), flowers and bark (4% each), sap (3%), stem and tuber (2% each), and seeds (1%) and the main mode of preparations are decoction (39%). Breynia androgyna (L.) Chakrab.dan N.P.Balakr., Moringa oleifera Lam, Jatropha multifida L., Zingiber montanum (J.Koenig) Link ex A.Dietr., Zingiber officinale Roscoe., and Tagetes erecta L. had the highest UV value (0.96), indicating the high importance of such species for the community. The high informant consensus factor (ICF=0.978) was assigned to the diseases of the blood and blood-forming organs and certain disorders involving the immune mechanism (DBF) category. The results of this study can enrich the existing study of medicinal uses of plant by communities, in this context is the use by a community in Aceh.
Physicochemical property of oil palm leaves and utilization of cellulose microfiber as probiotic encapsulant
Authors: Pato Usman , AYU DEWI FORTUNA , RIFTYAN EMMA , RESTUHADI FAJAR , PAWENANG WASISSO TUNGGUL , FIRDAUS ROYYAN , RAHMA ANNISA , SURONO INGRID SURYANTI , JASWIR IRWANDI
Published: August 2021

Abstract. Pato U, Ayu DF, Riftyan E, Restuhadi F, Pawenang WT, Firdaus R, Rahma A, Surono IS, Jaswir I. 2021. Physicochemical property of oil palm leaves and utilization of cellulose microfiber as probiotic encapsulant. Biodiversitas 22: 2937-2944. The vast land of oil palm (Elaeis guineensis) in Indonesia has a huge potential for oil palm solid waste, which can be used for various human needs. The physiochemical analysis performed was proximate analysis and fiber content, FTIR and X-ray diffraction analysis, an in vitro test on viability and resistance to acid and bile during storage at room and refrigerated temperatures. The main content of oil palm leaves was carbohydrates, especially fiber and followed by ash, protein, and fat. Fiber from oil palm leaves is mainly composed of lignin followed by cellulose and hemicellulose. X-ray diffraction analysis showed that the crystal index of cellulose from oil palm leaves was 10.1%. FTIR analysis showed that the enormous absorption value, which was the stretching vibrations of the -OH group ranging from 2919.17 to 2914.82 cm-1. Cellulose microfiber from oil palm leaves maintained the viability of L. fermentum InaCC B 1295 for up to 28 d of storage at room and refrigerated temperatures. The survival of strain B1295 at low pH and presence of bile was very high, characterized by a decrease in the number of cells by less than 0.5 log CFU/mL during storage of 35 d at room and refrigerated temperatures. Therefore, Cellulose microfiber from oil palm leaves has the potential to be used as an encapsulant for probiotics.
Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance
Authors: Basundari Fransiska Renita Anon , Sulistyaningsih Endang , Murti Rudi Hari , Nuringtyas Tri Rini
Published: August 2021

Abstract. Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species includes numerous cultivars with unique characteristics, such as harvesting time, yield, taste, odor, and bulb color. Tuk Tuk and Trisula, the cultivars used in this experiment, have different bulb colors. Tuk Tuk’s bulb color is light red, while that of Trisula is dark red. This color difference may be attributed to variations in the metabolite contents of the shallot bulbs. The present experiment aimed to reveal differences in the metabolite contents of the two shallot cultivars by using Nuclear Magnetic Resonance (NMR) and then determine the specific metabolites contributing to these differences. Bulbs of Tuk Tuk and Trisula were planted in the field under the same conditions and then collected. The bulb samples were powdered, freeze-dried, and subjected to a two-phase extraction method with CDCl3 and D2O as solvents to separate the nonpolar metabolites from polar ones. The extracts were analyzed by 1H-NMR, and the spectra collected were analyzed using MNOVA software and Metaboanalyst.ca. A total of 23 metabolites were successfully identified and characterized in this experiment. The contents of eight of these metabolites, namely, sucrose, glutamine, citric acid, choline, methiin, propiin, threonine, and formic acid, were significantly higher in Trisula than in Tuk Tuk. These differences may be correlated with variations in the color intensity, pungency, and other traits of the cultivars. The results demonstrate that NMR metabolite profiling could effectively differentiate metabolite profile variations among shallot cultivars.
Evaluation of heavy metal content in water and removal of metals using native isolated bacterial strains
Authors: Ibrahim Laila , RASHED REZAN OMER , MUHAMMED SIRWAN MUHSIN
Published: August 2021

Abstract. Saleh LIF, Rashed RO, Muhammed SM. 2021. Evaluation of heavy metal content in water and removal of metals using native isolated bacterial strains. Biodiversitas 22: 3163-3174. Natural surface water sources are susceptible to chemical contamination, including heavy metals. The goals of this study were to isolate naturally occurring heavy metal-tolerant bacteria by evaluating their ability to remove heavy metals from Tanjaro River and assessing the river’s water quality. The potency of bacteria to remove heavy metals (Cd, Pb, Cu, Cr, Ni, Zn, Co, and Fe) from the medium was conducted by inductively coupled plasma-optical emission spectrometry. Results of physicochemical parameters of the water samples were as follows: temperature 11.9–31°C, pH 6.1–8.64, total dissolved solids 268–464 mg.l-1, total hardness 232–485 mg.l-1, and alkalinity 122–324.3 mg.l-1. The overall mean concentrations of the heavy metals were in the order of Zn >Cu> Pb> Ni> Co> Fe> Cr> Cd), with values of 0.086, 0.073, 0.71, 0.068, 0.051, 0.056, 0.031, and 0.024ppm, respectively Moreover, the concentrations were generally exceeded the water quality criteria of WHO in some observations. The isolates included both gram-negative (56.5%) and gram-positive (43.5%) bacteria. The isolates displayed different degrees of resistance to heavy metals with maximum tolerable concentrations (MTCs) ranging from (10-250) ppm. Bacillus safensis and Leucobacter chromiiresistens were respectively able to tolerate (80, 90 ppm) Cd, (250, 160 ppm) Pb, (210,100 ppm) Cr, (110, 90 ppm) Ni and (160, 170 ppm) Co, while, Proteus mirabilis could tolerate 90 ppm Cd. The isolates were able to remove 55.4%Pb, 53.1%Cr, 53.7%Ni, 47.7Fe, 61.4%Co, and 55.8% Cu from a medium supplemented with the mixture of these heavy metals. The finding of this study indicated that Tanjaro River was undergoing frequent changes in water quality. Native bacterial isolates possessed considerable tolerance to selected heavy metals, which provides promising candidates for use in bioremediation.
Short Communication: New insights into the morphology and distribution of the Philippine endemic Etlingera pilosa (Zingiberaceae)
Authors: Naive Mark Arcebal , Demayo Cesar , Alejandro Grecebio Jonathan
Published: August 2021

Abstract. Naive MAK, Demayo CG, Alejandro GJD. 2021. Short Communication: New insights into the morphology and distribution of the Philippine endemic Etlingera pilosa (Zingiberaceae). Biodiversitas 22: 3175-3179. Etlingera pilosa Poulsen & Docot, only known in Negros Oriental and Negros Occidental of Visayas islands, Philippines, is recorded in the island of Mindanao for the first time, thus, extending its distributional range. The species is characterized by having sericeous ligule; leaves subsessile, narrowly obovate, coriaceous, glabrous adaxially, densely pubescent abaxially; inflorescence up to 12 cm long; flowers yellow, 3.0–3.2 cm long; subglobose to obovoid, pilose, with wart like blunt spines fruit; and seeds irregularly subglobose to oblate, black, fully embedded in juicy sour-sweet white aril. This species closely resembles E. pubimarginata but differs in having densely pubescent abaxial leaf, sericeous ligule and subglobose to obovoid, pilose, with wart like blunt spines fruit. Here, we provide a detailed description, comparison with its allied species, colored photographs to aid identification, a distribution map, an IUCN conservation assessment and a taxonomic key to the Mindanao Etlingera species.
The status of the Dactylorhiza incarnata populations in the Kalba Altai, Kazakhstan
Authors: Danilova Alevtina , Sumbembayev Aidar
Published: August 2021

Abstract. Danilova AN, Sumbembayev AA. 2021. The status of the Dactylorhiza incarnata populations in the Kalba Altai, Kazakhstan. Biodiversitas 22: 3180-3195. The article provides a botanical description of the current state of populations of threatened species Dactylorhiza incarnata (L.) Soo in the Kalba. The ecological analysis of the flora, the ecological optimum of the species, the vitality of plant communities, and the degree of anthropogenic load are presented in the research. On the basis of field surveys, four main populations were identified in 13 typical plant communities. Flora of plant communities of D. incarnata on the Kalba Ridge includes 117 species belonging to 30 families and 80 genera. The ecological optimum of D. incarnata can be spotted on the southeastern slopes with diffused lighting and moderate wind impact. The plant communities with optimal conditions are Filipendulo-Sanguisorbo-Thalictrosum and Carexoto-Equiseto-Filipendulo-Festucosum characterized by highly humid meadows and forest edges with low density and competition. Self-maintenance and reproduction of the species are fulfilled mainly by seeds, less often by vegetative reproduction of the finger root. Due to analysis of the age composition of populations and the dynamics and stability of plant communities the recovery index is within the optimum. Typical indicator species are Thalictrum simplex L., Salix viminalis L., Filipendula ulmaria (L.) Maxim., Sanguisorba officinalis L., Geranium pratense L., Festuca pratensis Huds.
The genetic diversity and reproductive dynamics of sandalwood in Gunung Sewu (Java, Indonesia) in 2012-2019: designing conservation strategies in a continuous versus fragmented landrace
Authors: Ratnaningrum Yeni , Indrioko Sapto , Karrin Amellita , Kurniawan Aditya , PUTRI ANGGI D.C.
Published: August 2021

Abstract. Ratnaningrum YWN, Indrioko S, Karrin A, Kurniawan A, Putri ADC. 2021. The genetic diversity and reproductive dynamics of sandalwood in Gunung Sewu (Java, Indonesia) in 2012-2019: designing conservation strategies in a continuous versus fragmented landrace. Biodiversitas 22: 3219-3229. This research aimed to compare the dynamics of the genetic diversity, mating systems, and reproductive outputs of the most fragmented (Bejiharjo) and continuous (Bleberan) sandalwood landraces in Gunung Sewu in the flowering periods of 2012-2019. Among the different sandalwood landraces found in Gunung Sewu, Bejiharjo has the highest levels of santalol; unfortunately, this landrace also requires the most attention because of heavy habitat fragmentation resulting from overexploitation, urbanization, and cave-tourism activities. Compared with Bejiharjo, Bleberan demonstrated a wider genetic base and greater outcrossing. Mature and flowering individuals in Bejiharjo decreased from 2012 to 2019 as fragmentation increased, particularly in 2019, during which extreme individual reduction due to illegal logging and land conversion was noted. Recent years consisted of more-clonalized stands, as indicated by the finding of fewer distinct genotypes and higher clonality rates. Increases in clonality and narrowing of the genetic base over time increased selfing rates and reduced offspring heterozygosity and seedling recruitment. The dynamics of genetic diversity in Bleberan, which was relatively undisturbed, appeared to be highly affected by fluctuations in the number of parental genetic bases (i.e., flowering parents) in each flowering season. Some parents bore flowers in one flowering season but remained in a vegetative state in the following season. This irregularity of flowering individuals caused fluctuations in actual population size, resulting in a different genetic base, mating system, and reproductive outputs in every season. All six loci (i.e., Dia-1, Dia-2, Est-1, Est-2, Est-3, and Skd-1) examined in Bleberan are polymorphic sites, and no missing alleles were noted in any of the observation years. Est-2 was monomorphic in Bejiharjo, where rare and missing alleles were more apparent, particularly at the seedling level. Genetic differentiation over time was observed between Bejiharjo and Bleberan, and increased fragmentation was noted in Bejiharjo. The migration rate between these two landraces also decreased over time. Focusing on maintaining the reproductive and genetic processes of each population by implementing different strategies based on the genetic base, mating systems, and degree of fragmentation and clonality of the landrace is recommended.
Short Communication: Nematodes associated with Robusta coffee plantations in Malang District, East Java, Indonesia
Authors: TARNO HAGUS , MARSUDI EYIK WIDYANSYAFICHA , WIDJAYANTI TITI , Setiawan Yogo
Published: August 2021

Abstract. Tarno H, Marsudi EW, Widjayanti T, Setiawan Y. 2021. Short Communication: Nematodes associated with Robusta coffee plantations in Malang District, East Java, Indonesia. Biodiversitas 22: 3306-3312. Coffee (Coffea spp.) is an important commodity in Indonesia. Nematodes have different roles, such as plant parasites and non-parasites, and are commonly found in the soil of coffee plantations. This research determined the ecological role, diversity, and abundance of nematodes in three coffee plantations in Malang District, East Java. We used a Whitehead tray to extract and isolate nematodes from the soil and root samples. Nematodes were identified based on their morphological characteristics. Analysis of variance and diversity indices were used to identify the differences among three coffee plantations. We identified eight nematode genera in the Ngantang sub-district site. In the Jatikerto Agrotechnopark and University of Brawijaya Forest sites, six genera were identified. Criconemoides, Pratylenchus, Xiphinema, Helicotylenchus are plant-parasitic nematodes, and Mononchus, Dorylaimus, Rhabditis, and Aphanolaimus are non-plant parasitic nematodes. Non-plant parasitic nematodes were more abundant than plant-parasitic nematodes in all sites. Non-plant parasitic nematode, Dorylaimus was the dominant genus in this study (272 individuals), ca. 35% of the total nematodes collected. In this study, the differences between coffee plantations and soil pH influence nematode abundance. The nematode abundance increases when the soil pH is lower.
A spatial model of forest and land fire vulnerability level in the Dairi District, North Sumatra, Indonesia
Authors: Thoha Achmad Siddik , Triani Hesty
Published: August 2021

Abstract. Thoha AS, Triani H. 2021. A spatial model of forest and land fire vulnerability level in the Dairi District, North Sumatra, Indonesia. Biodiversitas 22: 3319-3326. Fires often occur every dry season and have a significant impact on ecosystems and human activities. One of the important roles in reducing the risk of forest and land fires is the availability of updated vulnerability level maps in all vulnerable areas. The objective of this study was to determine the relationship between the driving factors for forest and land fires and hotspots and to obtain a spatial model of the distribution of vulnerability to forest and land fires in the Dairi District of North Sumatra Province. This study uses a composite mapping analysis method to obtain a spatial model and the distribution of vulnerable areas to forest and land fires. Six variables in the form of maps were used in building the model, including land cover, population density, distance from the road, distance from the river, and distance from the settlement. This study showed that the most important variable for vulnerability level model of forest and land fires was the distance from the settlement. This study also found that open land, the farthest distance to the road, the farthest distance to the river, the farthest distance to the settlement, and the densest population were the driving factors for increased fire activity. The spatial model of the vulnerability level to forest and land fires in Dairi District was Y = 0.022X1 + 0.214X2 + 0.113X3 + 0.482X4 + 0.169X5. Land cover having high-very high vulnerability level belonged to open land dominated by grass. The largest areas with a high-very high forest fire vulnerability level in Dairi District were spread over Tanah Pinem Sub-district.
Co-infection with Trichodina (Ciliophora: Trichodinidae) and Aeromonas caviae synergistically changes the hematology and histopathology of Asian seabass Lates calcarifer
Authors: Sufardin Sufardin , Sriwulan Sriwulan , Anshary Hilal
Published: August 2021

Abstract. Sufardin, Sriwulan, Anshary H. 2021. Co-infection with Trichodina (Ciliophora: Trichodinidae) and Aeromonas caviae synergistically changes the hematology and histopathology of Asian seabass Lates calcarifer. Biodiversitas 22: 3371-3382. Consequential interaction contributed by parasitic and bacterial infections in fish has received little attention and impact of co-infection is mostly undescribed. This study identifies and describes notable damage arising from the co-infection of Trichodina and Aeromonas caviae infecting the seabass Lates calcarifer. A completely randomized experiment was performed with 4 infection treatments (healthy fish; fish naturally infected with Trichodina sp.; healthy fish injected with A. caviae; fish naturally infected with Trichodina sp. and injected with A. caviae) and 3 replicates. Fish were obtained from the Takalar Brackish Aquaculture Institute, South Sulawesi, Indonesia. The data were statistically tested using linear regression analysis. The results showed bacterial pathogenicity, lymphocyte percentage, and histopathological quantification were statistically different (P < 0.05). Meanwhile, the number of erythrocytes and leukocytes, the percentages of monocytes and neutrophils were not significantly different (P > 0.05) between treatments. The non-infected fish showed no cell inflammation and necrosis, very little hemorrhage (liver and gills), negligible hemorrhage and melano-macrophages (kidney). Kidneys and liver were the most damaged organs of co-infected fish, with a large number of inflammatory cells, hemorrhages, vacuoles, melano-macrophages, scar tissue, inflammation and necrosis. Infection with Trichodina sp. presented less damage than the co-infection of A. caviae and Trichodina sp. In conclusion, single infection showed a mild pathological impact, meanwhile, the co-infection of Trichodina sp and A. caviae contribute significantly to fish’s health.
Effectiveness of green betel leaf and lime extract against Staphylococcus aureus and Escherichia coli
Authors: Gloria R.Y , YULIYANI RAHMA , ASROR MIA MAR'ATUSH SHOLIHA
Published: August 2021

Abstract. Gloria RY, Yuliyani R, Asror MMS. 2021. Effectiveness of green betel leaf and lime extract against Staphylococcus aureus and Escherichia coli. Biodiversitas 22: 3452-3457. Indonesian society utilizes biodiversity as source of medicinal herbs. Betel leaf and lime are included in traditional Indonesian medicinal plants that have antibacterial and antioxidant properties. One of the benefits of herbal medicinal plants of betel leaf and lime is that it can be used as a natural hand sanitizer. The purpose of this study was to test the effectiveness of naturally made hand sanitizer against Staphylococcus aureus and Escherichia coli by the combination of green betel leaf extract and lime extract. The experiment was performed by the Kirby-Bauer disc diffusion method. Results showed that a combination of 50% betel leaf extract and 50% lime extract inhibited the growth of S. aureus whereas, 75% betel leaf extract and 100% lime extract exhibited strong inhibition than 70% alcohol.
Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand
Authors: Saensouk Surapon , Saensouk Piyaporn
Published: August 2021

Abstract. Saensouk S, Saensouk P. 2021. Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand. Biodiversitas 22: 3458-3466. Comprehensive karyotype analysis of three species of Allium from Thailand has not been reported. This work aims to study karyotype analysis of three species of Allium from Thailand. It is confusing due to the use of the common name or local name or local Thai name and morphologies. The karyotype analysis of three species with two variations in the genus Allium from Thailand were studied from root tips and observed under a microscope then karyotype was obtained from 10 metaphase plates. The chromosome numbers of them were found 2n = 16. The karyotype formulas of them were constructed as 14m + 2st (Allium ascalonicum L. or shallot), 12m + 2sm + 2st (A. cepa L. or onion), 10m + 6sm (1 sat) (A. cepa L. or red onion), 10m + 4sm + 2st (A. sativum L. or big garlic or Chinese garlic) and 8m + 6sm + 2st (A. sativum L. or small garlic or Thai garlic). Chromosome structure differences among the three species appeared in the number of m, sm and st chromosomes and the satellites at the end of the short arm of A. cepa L. (red onion). This is new data on chromosome structure of the genus Allium. Moreover, the karyotype formulas, chromosome structures and satellites of this study should be used for classification of Allium from Thailand.
Mangrove diversity and community structure of Mimika District, Papua, Indonesia
Authors: Setyadi Gesang , PRIBADI RUDHI , WIJAYANTI DIAH PERMATA , SUGIANTO DENNY NUGROHO
Published: August 2021

Abstract. Setyadi G, Pribadi R, Wijayanti DP, Sugianto DN. 2021. Mangrove diversity and community structure of Mimika District, Papua, Indonesia. Biodiversitas 22: 3562-3571. Despite being one the largest mangrove forest in the world, there are few studies on mangrove diversity and community structure in Mimika, Papua Province. The study collected data from 4 estuaries of Mimika Region, i.e. Kamora, Tipuka, Ajkwa and Minajerwi. Mangrove species, tree diameter at breast height and canopy height data were collected in 10 m x 10 m plot with three replicates for distance, specifically 0, 250, and 500m perpendicular to the riverbank. This study found 66 mangroves species consisting of 20 major, 10 minor, and 36 associate mangrove species. Their density ranged from 577-1,345 trees ha-1 with diversity Shannon-Weiner diversity indices (H’) ranging from 0.62-1.19 and Evenness Index (J’) ranging from 0.09-0.18. The canopy height of the mangrove trees was ranged from 9-42.5 m. The highest density species was Rhizophora apiculata (333 ha-1) followed by Avicennia marina, Bruguiera gymnorrhiza, and Camptostemon schultzii, while B. parviflora, S. alba, and X. granatum were the least dominant. Regarding basal area, R. apiculata and B. gymnorrhiza were the highest with medians of 7,853 cm2 ha-1 and 5,201 cm2 ha-1 respectively. In the low tidal zone area, mangrove was dominated by A. marina, Sonneratia alba and R. mucronata. Medium low to medium high tidal zones were dominated by B. gymnorrhiza, R. apiculata, and B. parviflora. The high tidal zone was more dominated by Nypa fruticans.
A social-ecological system approach to Bali cattle raising in Timor Island, Indonesia
Authors: Firman Achmad , NONO OBED HABA
Published: August 2021

Abstract. Firman A, Nono OH. 2021. A social-ecological system approach to Bali cattle raising in Timor Island, Indonesia. Biodiversitas 22: 3585-3593. Cattle raising in East Nusa Tenggara play important role in the daily life of local people including to fulfill nutritional needs, to generate cash income, to develop social relationships, and to maintain religious activities. On the island of Timor, the type of cattle mostly kept by farmers is Bali cattle. This type of cattle perfectly suits the environmental and social conditions of the island. The purpose of this study was to investigate the interaction of social-ecological systems (SESs) and other systems that were able to maintain the sustainability of Bali cattle raising in Timor Island.  All components in the system that interact with each other were studied through in-depth interviews with informants (15 cattle farmers, five community leaders, five cattle traders, and 10 staff of livestock services district and province levels). The results showed that the SESs approach could provide an explanation on the relationship between resource systems, resource units, governance systems, and users as well as systems that are outside SESs, namely the market and government policies, which altogether were able to improve Bali cattle raising sustainability. Farmers had an important role in Bali cattle raising and their habitat environment. Local and export markets played a role in providing value for Bali cattle raising. The local government maintained the balance of supply and demand for Bali cattle through the East Nusa Tenggara Governor Regulations No. 78 of 2019. Therefore, the research has succeeded in identification of feed resources in savanna and steppe and provide an opportunity to increase the cattle population on the island of Timor. The interaction between farmers and their environment has been well established, however, it is necessary to improve the quality of forage feed in grazing land.
Evaluation of bilateral interdigitated Pacman flap for reconstruction of skin defects in different parts of the body
Authors: Albayati WK , Qassim YN
Published: August 2021

Restorative surgical repair using different surgical modalities is required on the excision of skin lesions or when treating traumatic lacerations; each modality has advantages and drawbacks. In this article, we evaluate the efficacy of using a bilateral interdigitated Pacman flap to reconstruct circular and oval cutaneous defects on different parts of the body. Fourteen patients with soft tissue defects on different parts of the body underwent reconstruction using a bilateral interdigitated Pacman flap. The design of this flap is similar to that of a conventional bilateral V–Y advancement flap; however, the limbs of the V are drawn as slightly curved convex lines. All patients were followed-up for more than 6 months. All soft tissue defects were reconstructed completely using this technique, and no significant complications were noticed except in one patient who developed partial flap necrosis. However, aesthetic issues remain a limiting factor in using this technique. This flap is a reliable, practical and effective option for closure of circular and oval soft tissue defects, although there are certain limitations when used on the face and in younger patients.
Basilar Invagination: Surgical Treatment by Novel Anterior Implant
Authors: Patkar Sushil V , Patkar Pradnya
Published: August 2021

Introduction: Anterior retropharyngeal realignment, distraction, and atlantoaxial fixation are an option for the treatment of symptomatic basilar invagination (BI). The anterior implants for distraction and fixation for atlantoaxial joints are still evolving. We share our experience using a novel implant which can easily, safely, and rigidly fix both lateral masses to the body of the axis.
Methods: After exposing both the atlantoaxial joints anteriorly, the joints were prepared, distracted with wedge shaped autologous tricorticate bone grafts and realigned to correct the cervicomedullary strain. The atlantoaxial joints were fixed using a novel titanium plate by passing screws upwards and laterally into the lateral masses of the atlas and centrally into the body of the axis. Post-operative imaging showed effective correction of BI and atlantoaxial dislocation. Post-operative dynamic X-ray images confirmed maintenance of rigid fixation at 6 months.
Conclusion: This new plate screw construct is safe, easy, cost-efficient, and biomechanically appealing option for the treatment of symptomatic BI.
Keywords: Basilar invagination, atlantoaxial dislocation, vertebral artery injury, atlantoaxial fixation, atlantoaxial instability.
Metastatic Adenocarcinoma of Lungs, Mimicking Primary Scapular Tumor: A Case Report and Review of Literature
Authors: Macwan Anson Albert , Nanda Saurav Narayan , Mishra Debasish , Purohit Ashish , Patel Eshwar , Gachhayat Ashok
Published: August 2021

Introduction: Cancer metastasis to the scapula and tibia is an infrequent clinical presentation. Lung cancer is one of the leading causes of death in the world. Around 80–85% of lung cancer are non-small cell lung cancer and around 30–35% of them are adenocarcinoma which metastasize to bone. Scapular metastasis is a rare event and presents with bone pain.
Case Report: In this case report of 56-year-old male, presented with swelling to the scapula as a first symptom followed by swelling in the tibia. On further investigation, it was found to be multiple metastasis primaries as an adenocarcinoma of the lung. He was managed with palliative chemotherapy, which enhanced the patient’s quality of life, slowing down the disease process and clinical improvement.
Conclusion: Lung cancer is the most common cancer which is progressing rapidly and spreading commonly to bones. When patients present with a mass or any suspicion of tumor, careful clinical examination and diagnostic tools often catch malignancy.
Key words: Scapular metastasis, adenocarcinoma lung, bone metastasis, palliative chemotherapy, non-small cell lung carcinoma.
Coherent self-control of free-space optical beams with integrated silicon photonic meshes
Authors: Milanizadeh Maziyar , Toso Fabio , Ferrari Giorgio , Jonuzi Tigers , Miller David A. B. , Melloni Andrea , Morichetti Francesco
Published: August 2021

In technologies operating at light wavelengths for wireless communication, sensor networks, positioning, and ranging, a dynamic coherent control and manipulation of light fields is an enabling element for properly generating and correctly receiving free-space optical (FSO) beams even in the presence of unpredictable objects and turbulence in the light path. In this work, we use a programmable mesh of Mach–Zehnder (MZI) interferometers to automatically control the complex field radiated and captured by an array of optical antennas. The implementation of local feedback control loops in each MZI stage, without global multivariable optimization techniques, enables an unlimited scalability. Several functionalities are demonstrated, including the generation of perfectly shaped beams with nonperfect optical antennas, the imaging of a desired field pattern through an obstacle or a diffusive medium, and the identification of an unknown obstacle inserted in the FSO path. Compared to conventional devices used for the manipulation of FSO beams, such as spatial light modulators, our programmable device can self-configure through automated control strategies and can be integrated with other functionalities implemented onto the same photonic chip.
Ultrabroadband and multiband infrared/terahertz photodetectors with high sensitivity
Authors: Zhu Jiaqi , Zhu He , Liu Mengjuan , Wang Yao , Xu Hanlun , Ali Nasir , Deng Huiyong , Tan Zhiyong , Cao Juncheng , Dai Ning , Wu Huizhen
Published: August 2021

Broadband response is pursued in both infrared (IR) and terahertz (THz) detection technologies, which find their applications in both terrestrial and astronomical realms. Herein, we report an ultrabroadband and multiband IR/THz detector based on blocked-impurity-band detecting principle. The detectors are prepared by implanting phosphorus into germanium (Ge:P), where photoresponses with a P impurity band, a self-interstitial defect band, and a vacancy-P (V-P) pair defect band are realized simultaneously. The response spectra of the detectors show ultrabroad and dual response bands in a range of 3–28 μm (IR band) and 40–165 μm (THz band), respectively. Additionally, a tiny mid-IR (MIR) band within 3–4.2 μm is embedded in the IR band. The THz band arises from the P impurity band, whereas the IR and the MIR bands are ascribed to the two defect bands. At 150 mV and 4.5 K, the peak detectivities of the three bands are obtained as

2.9
×

10

12

Jones (at 3.9 μm),

6.8
×

10

12

Jones (at 16.3 μm), and

9.9
×

10

12

Jones (at 116.5 μm), respectively. The impressive coverage and sensitivity of the detectors are promising for applications in IR and THz detection technologies.
Genotypic and phenotypic diversity of Mycobacterium tuberculosis complex genotypes prevalent in West Africa
Authors: Osei-Wusu Stephen , Otchere Isaac Darko , Morgan Portia , Musah Abdul Basit , Siam Ishaque Mintah , Asandem Diana , Afum Theophilus , Asare Prince , Asante-Poku Adwoa , Kusi Kwadwo Asamoah , Gagneux Sebastien , Yeboah-Manu Dorothy
Published: August 2021

Findings from previous comparative genomics studies of the Mycobacterium tuberculosis complex (MTBC) suggest genomic variation among the genotypes may have phenotypic implications. We investigated the diversity in the phenotypic profiles of the main prevalent MTBC genotypes in West Africa. Thirty-six whole genome sequenced drug susceptible MTBC isolates belonging to lineages 4, 5 and 6 were included in this study. The isolates were phenotypically characterized for urease activity, tween hydrolysis, Thiophen-2-Carboxylic Acid Hydrazide (TCH) susceptibility, nitric oxide production, and growth rate in both liquid (7H9) and solid media (7H11 and Löwenstein–Jensen (L-J)). Lineage 4 isolates showed the highest growth rate in both liquid (p = 0.0003) and on solid (L-J) media supplemented with glycerol (p<0.001) or pyruvate (p = 0.005). L6 isolates optimally utilized pyruvate compared to glycerol (p<0.001), whereas L5 isolates grew similarly on both media (p = 0.05). Lineage 4 isolates showed the lowest average time to positivity (TTP) (p = 0.01; Average TTP: L4 = 15days, L5 = 16.7days, L6 = 29.7days) and the highest logCFU/mL (p = 0.04; average logCFU/mL L4 = 5.9, L5 = 5.0, L6 = 4.4) on 7H11 supplemented with glycerol, but there was no significant difference in growth on 7H11 supplemented with pyruvate (p = 0.23). The highest release of nitrite was recorded for L5 isolates, followed by L4 and L6 isolates. However, the reverse was observed in the urease activity for the lineages. All isolates tested were resistant to TCH except for one L6 isolate. Comparative genomic analyses revealed several mutations that might explain the diverse phenotypic profiles of these isolates. Our findings showed significant phenotypic diversity among the MTBC lineages used for this study.
Evaluation of novel LCI CAD EYE system for real time detection of colon polyps
Authors: Neumann Helmut , Kreft Andreas , Sivanathan Visvakanth , Rahman Fareed , Galle Peter R.
Published: August 2021

Background

Linked color imaging (LCI) has been shown to be effective in multiple randomized controlled trials for enhanced colorectal polyp detection. Recently, artificial intelligence (AI) with deep learning through convolutional neural networks has dramatically improved and is increasingly recognized as a promising new technique for enhancing colorectal polyp detection.

Aim

This study aims to evaluate a newly developed computer-aided detection (CAD) system in combination with LCI for colorectal polyp detection.

Methods

First, a convolutional neural network was trained for colorectal polyp detection in combination with the LCI technique using a dataset of anonymized endoscopy videos. For validation, 240 polyps within fully recorded endoscopy videos in LCI mode, covering the entire spectrum of adenomatous histology, were used. Sensitivity (true-positive rate per lesion) and false-positive frames in a full procedure were assessed.

Results

The new CAD system used in LCI mode could process at least 60 frames per second, allowing for real-time video analysis. Sensitivity (true-positive rate per lesion) was 100%, with no lesion being missed. The calculated false-positive frame rate was 0.001%. Among the 240 polyps, 34 were sessile serrated lesions. The detection rate for sessile serrated lesions with the CAD system used in LCI mode was 100%.

Conclusions

The new CAD system used in LCI mode achieved a 100% sensitivity per lesion and a negligible false-positive frame rate. Note that the new CAD system used in LCI mode also specifically allowed for detection of serrated lesions in all cases. Accordingly, the AI algorithm introduced here for the first time has the potential to dramatically improve the quality of colonoscopy.