bluuebunny/crossref_metadata_2025 huggingface dataset:
Gender heterogeneity in dyslipidemia prevalence, trends with age and associated factors in middle age rural Chinese
Authors: Wang Minmin , Liu Mengfei , Li Fenglei , Guo Chuanhai , Liu Zhen , Pan Yaqi , Liu Ying , Liu Fangfang , Cai Hong , Wu Yangfeng , He Zhonghu , Ke Yang
Published: June 2020

Background

Heterogeneity should be carefully addressed to facilitate establishment of effective population-level blood lipid management. The primary aim of the study was to investigate gender heterogeneity in prevalence of dyslipidemia, including trends with age and associated factors in middle age rural Chinese.

Methods

This is a cross-sectional study based on a baseline investigation of a population-based randomized controlled trial in rural China, involving 26,378 permanent residents of age 45–69. The age-specific prevalence of dyslipidemia was estimated for men and women, and the trends of prevalence with age were compared. Logistic regression was used to explore the factors associated with prevalent risk of dyslipidemia.

Results

The overall prevalence of dyslipidemia was significantly higher in females than in males for borderline high and above (BHA) total cholesterol (TC ≥ 200 mg/dL), BHA triglycerides (TG ≥ 150 mg/dL) and BHA low-density lipoprotein cholesterol (LDL-C ≥ 130 mg/dL), but was lower for low high-density lipoprotein cholesterol (HDL-C < 40 mg/dL) in females than the corresponding prevalence in males. The prevalence of borderline high and above TC, TG and LDL-C all rose with age in females, but was stable or even decreased with age in males. In contrast, graphic representation of the prevalence of low HDL-C showed no striking age related trend in both genders. Risk of dyslipidemia was associated predominantly with obesity in males, but was more predominantly associated with hypertension in females.

Conclusion

Heterogeneity was found in comparing the prevalence of dyslipidemia in men and women, and gender heterogeneity was found in its trend with age and associated factors in middle aged rural Chinese. The effectiveness of population-level blood lipid management and CVD primary prevention programs in China is expected to be improved if gender heterogeneity is considered.
A focused netnographic study exploring experiences associated with counterfeit and contaminated anabolic-androgenic steroids
Authors: Frude Evelyn , McKay Fiona H. , Dunn Matthew
Published: June 2020

Background

A primary consequence of illicit drug markets and the absence of regulation is the variable quality or purity of the final product. Analysis of anabolic-androgenic steroid seizures shows that these products can contain adulterated products, product not included on the label, or product of unsatisfactory standard. While the potential negative effects of counterfeit anabolic-androgenic steroids (AAS) use is a recognised risk associated with use, no study has explored personal experiences associated with use. The aim of the present study was to use online discussion forums to investigate and explore the experiences associated with the purchase and consumption of counterfeit AAS among consumers.

Methods

An online search was conducted to identify online forums that discussed counterfeit or contaminated AAS; three were deemed suitable for the study. The primary source of data for this study was the ‘threads’ from these online forums, identified using search terms including ‘counterfeit’, ‘tampered’, and ‘fake’. Threads were thematically analysed for overall content, leading to the identification of themes.

Results

Data from 134 threads (2743 posts from 875 unique avatars) was included. Two main themes were identified from the analysis: (1) experiences with counterfeit product and (2) harms and benefits associated with counterfeit product.

Conclusions

The use of counterfeit or contaminated substances represents a public health concern. Those who report using performance and image enhancing drugs such as AAS for non-medical purposes report consuming these substances and experiencing harm as a result. Consumers take steps to limit coming into contact with counterfeit or contaminated product, though recognise that many of these have limitations. The implementation of accessible drug safety checking services may provide an opportunity to provide consumers with information to assist them with making healthier choices.
Development of the Thalassaemia Adult Life Index (ThALI)
Authors: Kantaris Xenya , Shevlin Mark , Porter John , Myers Lynn
Published: June 2020

Background

Beta Thalassaemia Major (βTM) is a chronic genetic illness whereby the challenges faced by patients exposes them to increased risk of psychosocial issues. Despite this, a disease-specific tool to measure the impact of this illness on adult patients has yet to be developed.

Methods

In collaboration with βTM adult patients, this study aimed to develop a comprehensive, disease-specific, easy to use psychometrically sound tool to measure the impact of chelation and transfusion dependent βTM in a cross-cultural patient group in England.The Thalassaemia Life Index (ThALI) was developed in two stages – item generation and pre-testing and item reduction – in collaboration with service users. Recruited adult patients shaped the design of the instrument including its statements and subscales. Standard item reduction techniques were used to develop the instrument.

Results

The final version of the ThALI encompasses 35 statements and five sub-scales - general physical health, coping, body image, appearance and confidence, social relationships and autonomy. This endorses the multidimensionality of quality of life (QoL). The factor structure of the ThALI is highly stable and its internal consistency is high (alpha = 0.87 for the overall scale; 0.83–0.94 for its subscales). The ThALI has sound scaling assumptions, acceptability and score variability. Content validity was confirmed by experts and service user interviewees. The loadings for the items retained were adequate and the item discriminant validity sound.

Conclusions

The ThALI covers the impact of βTM in adult patients. Preliminary testing shows its multidimensionality to be reliable and valid. The national authentication of the tool with patients treated in Centres of Excellence will aim to provide further evidence regarding the ThALI’s psychometric properties. Once authenticated, the ThALI may be utilised in research and in clinical settings to assess the effects of new therapies and/or interventions from the patients’ perspective to inform practice and/or to identify areas of concern.
Oral impacts on daily performances and its socio-demographic and clinical distribution: a cross-sectional study of adolescents living in Maasai population areas, Tanzania
Authors: Simangwa Lutango D. , Johansson Ann-Katrin , Johansson Anders , Minja Irene K. , Åstrøm Anne N.
Published: June 2020

Background

In a global perspective, oral health among adolescents has improved during recent decades. However, oral problems still persist especially in many underprivileged societies. This study aimed to estimate the prevalence of oral impacts in adolescents and to identify important clinical- and socio-demographic covariates. In addition, this study compares Maasai and non-Maasai adolescents regarding any association of socio-demographic and clinical covariates with oral impacts on daily performances.

Methods

A total of 989 adolescents were invited from 23 randomly selected public primary schools in Monduli and Longido districts, Tanzania. All adolescents attending 6th grade classes were invited to participate. A total of 930 accepted and of those 24 were excluded, leaving 906 (91.6%) participants for the study.

Results

A total of 143/906 (15.8%) had at-least one oral impact on daily performances (OIDP > 0), 14.6% among the Maasai and 20.5% among the non-Maasai ethnic group. Cluster adjusted logistic regression revealed that: adolescents from Longido district (OR = 0.4) and adolescents with good oral hygiene (OR = 0.7) were less likely to report OIDP> 0 and; non Maasai (OR = 1.6), those with least poor parents (OR = 2.0), DMFT> 0 (OR = 3.1) and those with positive answers to questions regarding TMD pain, 2Q/TMD > 0 (OR = 3.9) were more likely to report OIDP> 0. Stratified logistic regression by ethnicity revealed that, among the non-Maasais, older adolescents (OR = 3.7, 95% CI 1.1–12.8), those with DMFT> 0 (OR = 3.3 (1.2–9.0) and 2Q/TMD > 0 (OR = 9.0, 95% CI 3.3–25.0) were more likely to report at least one OIDP. The corresponding figures among the Maasais were (OR = 0.9, 95% CI 0.5–1.7), (OR = 2.8, 95% CI 1.4–5.5) and (OR = 3.0, 95% CI 1.7–5.2), respectively.

Conclusions

The prevalence of oral impacts was moderate but higher among the non-Maasai- than Maasai-adolescents attending rural primary schools in the Maasai population areas of Tanzania. This study also confirmed socioeconomic and oral clinical disparities in OIDP, some of which differed according to ethnicity. Caries experience and self-reported TMD pain associated more strongly with OIDP among the non-Maasais than among the Maasais. These results are important for public oral health decision makers who plan strategies for optimal primary oral health care and quality of life among adolescents belonging to minority groups in Tanzania.
Global prevalence estimates of three chronic musculoskeletal conditions: club foot, juvenile idiopathic arthritis and juvenile systemic lupus erythematosus
Authors: Dave Mona , Rankin Judith , Pearce Mark , Foster Helen E.
Published: June 2020

Background

Musculoskeletal (MSK) conditions are a major source of morbidity and disability. There is a lack of global comparable data on the burden of MSK conditions in children and young people. Our aim was to estimate the global prevalence of three MSK conditions - Talipes Equinovarus (Clubfoot), Juvenile Idiopathic Arthritis (JIA) and Juvenile Systemic Lupus Erythematosus (JSLE).

Methods

Using reported prevalence rates, age-stratified population data within the World Bank Data Bank in 2017 and United Nations country classification, we estimated the prevalence of these MSK conditions in < 5 year olds (clubfoot) and < 16 year olds (JIA and JSLE) across the world.

Results

We estimated that in 2017, there were ~ 675,061 < 5 year olds with clubfoot among 675,100,000 < 5 year olds, ~ 2,069,246 < 16 year olds with JIA and ~ 206,931 < 16 year olds with JSLE per 2,069,000,000 < 16 year olds, totalling ~ 2,951,238 with one of these conditions. Disease prevalence was greatest in Asia (South Asia), followed by Africa, Americas, Europe and Oceania.

Conclusions

An estimated 3 million children and young people globally are currently living with either clubfoot, JIA or JSLE; many in Asia and Africa. Further work is needed urgently to engage with global stakeholders to work together to improve access to effective care for the many who are affected and reduce the otherwise adverse lifelong impact on their health, quality of life and the impact on society.
Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
Authors: Shaqura Mohammed , Li Li , Mohamed Doaa M. , Li Xiongjuan , Treskatsch Sascha , Buhrmann Constanze , Shakibaei Mehdi , Beyer Antje , Mousa Shaaban A. , Schäfer Michael
Published: June 2020

Background

Recently, mineralocorticoid receptors (MR) were identified in peripheral nociceptive neurons, and their acute antagonism was responsible for immediate and short-lasting (non-genomic) antinociceptive effects. The same neurons were shown to produce the endogenous ligand aldosterone by the enzyme aldosterone synthase.

Methods

Here, we investigate whether endogenous aldosterone contributes to inflammation-induced hyperalgesia via the distinct genomic regulation of specific pain signaling molecules in an animal model of Freund’s complete adjuvant (FCA)-induced hindpaw inflammation.

Results

Chronic intrathecal application of MR antagonist canrenoate-K (over 4 days) attenuated nociceptive behavior in rats with FCA hindpaw inflammation suggesting a tonic activation of neuronal MR by endogenous aldosterone. Consistently, double immunofluorescence confocal microscopy showed abundant co-localization of MR with several pain signaling molecules such as TRPV1, CGRP, Nav1.8, and trkA whose enhanced expression of mRNA and proteins during inflammation was downregulated following i.t. canrenoate-K. More importantly, inhibition of endogenous aldosterone production in peripheral sensory neurons by continuous intrathecal delivery of a specific aldosterone synthase inhibitor prevented the inflammation-induced enhanced transcriptional expression of TRPV1, CGRP, Nav1.8, and trkA and subsequently attenuated nociceptive behavior. Evidence for such a genomic effect of endogenous aldosterone was supported by the demonstration of an enhanced nuclear translocation of MR in peripheral sensory dorsal root ganglia (DRG) neurons.

Conclusion

Taken together, chronic inhibition of local production of aldosterone by its processing enzyme aldosterone synthase within peripheral sensory neurons may contribute to long-lasting downregulation of specific pain signaling molecules and may, thus, persistently reduce inflammation-induced hyperalgesia.
Pressure pain threshold and temporal summation in adults with episodic and persistent low back pain trajectories: a secondary analysis at baseline and after lumbar manipulation or sham
Authors: Aspinall Sasha L. , Jacques Angela , Leboeuf-Yde Charlotte , Etherington Sarah J. , Walker Bruce F.
Published: June 2020

Background

People with chronic low back pain (LBP) typically have increased pain sensitivity compared to healthy controls, however its unknown if pain sensitivity differs based on LBP trajectory at baseline or after manual therapy interventions. We aimed to compare baseline pressure pain threshold (PPT) and temporal summation (TS) between people without LBP, with episodic LBP, and with persistent LBP, and to compare changes over time in PPT and TS after a lumbar spinal manipulation or sham manipulation in those with LBP.

Methods

Participants were aged 18–59, with or without LBP. Those with LBP were categorised as having either episodic or persistent LBP. PPT and TS were tested at baseline. LBP participants then received a lumbar spinal manipulation or sham, after which PPT and TS were re-tested three times over 30 min. Generalised linear mixed models were used to analyse data.

Results

One hundred participants (49 female) were included and analysed. There were 20 non-LBP participants (mean age 31 yrs), 23 episodic LBP (mean age 35 yrs), and 57 persistent LBP (mean age 37 yrs). There were no significant differences in PPT or TS between groups at baseline. There was a non-significant pattern of lower PPT (higher sensitivity) from the non-LBP group to the persistent LBP group at baseline, and high variability. Changes in PPT and TS after the interventions did not differ between the two LBP groups.

Discussion

We found no differences between people with no LBP, episodic LBP, or persistent LBP in baseline PPT or TS. Changes in PPT and TS following a lumbar manual therapy intervention do not appear to differ between LBP trajectories.

Trial registration

The trial was prospectively registered with ANZCTR (ACTRN12617001094369).
On the applicability of the Tubulin-Based Polymorphism (TBP) genotyping method: a comprehensive guide illustrated through the application on different genetic resources in the legume family
Authors: Braglia Luca , Gavazzi Floriana , Morello Laura , Gianì Silvia , Nick Peter , Breviario Diego
Published: June 2020

Background

Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method suitable for assessing plant diversity.

Results

Exploiting one of the most valuable features of TBP, that is the convenient and immediate application of the assay to groups of individuals that may belong to different taxa, we show that the TBP method can successfully discriminate different agricultural species and their crop wild relatives within the Papilionoideae subfamily. Detection of intraspecific variability is demonstrated by the genotyping of 27 different accessions of Phaseolus vulgaris.

Conclusions

These data illustrate TBP as a useful and versatile tool for plant genotyping. Since its potential has not yet been fully appreciated by the scientific community, we carefully report all the experimental details of a successful TBP protocol, while describing different applications, so that the method can be replicated in other laboratories.
Stepwise external wrapping procedure for Stanford type A aortic dissection in extremely high-risk patients: case reports
Authors: Suematsu Yoshihiro , Nishi Satoshi , Arima Daisuke , Yoshimoto Akihiro
Published: June 2020

Background

Acute aortic dissection (AAD) is a rare, but a life-threatening condition which can lead to coronary, brachiocephalic or branch vessel malperfusion, as well as aortic valve insufficiency, or aortic rupture. Mortality of surgical treatment in high-risk or elderly patients with Type A Acute aortic dissection (TAAAD) still remains high, and treatment for such patients remains controversial.

Case presentation

A new surgical approach which entails “stepwise external wrapping (SEW)” using a zero-porosity artificial graft was developed in extremely high-risk patients with TAAAD. Herein, we described its surgical details and showed two representative cases which was successfully done.

Conclusions

Our SEW procedure is a feasible alternative to conventional graft replacement for TAAAD in extremely high-risk or aged patients, although the gold standard consists of surgical replacement of the dissected aorta. (129 words).
Juvenile xanthogranuloma as a new type of skin lesions in tuberous sclerosis complex
Authors: Lu Qian , Shi Xiu-Yu , Wang Yang-Yang , Zhang Meng-Na , Wang Wen-Ze , Wang Jing , Wang Qiu-Hong , Chen Hui-Min , Zou Li-Ping
Published: June 2020

Objective

Tuberous sclerosis complex (TSC) is a rare autosomal dominant genetic disease with many manifestations, and it involves any organ. In this study, we report a TSC patient with new type skin lesions.

Methods

A 7-month-old TSC boy with multiple cutaneous nodules was admitted in our hospital. We collected the clinical data of the patient. We performed biopsy of cutaneous nodules and whole-exome sequencing in both paraffin block tissue and blood samples.

Results

The patient presented with a 2 month history of gradual growth multiple cutaneous nodules. He had cardiac rhabdomyoma, subependymal giant cell astrocytoma (SEGA) and hypomelanotic macules. The pathological finding of cutaneous nodules was consistent with juvenile xanthogranuloma (JXG). After 3 months of sirolimus treatment, the multiple nodules disappeared. The whole-exome sequencing identified TSC1 (c.2356C > T, p.R786*) mutation in both paraffin block tissue and blood samples. We overturned the original pathological diagnosis and finally identified JXG as a new type of skin lesions in TSC.

Conclusion

This is the first report on the occurrence of JXG skin lesions in TSC patient. Genetic testing is necessary in JXG. These findings expand the phenotype of skin in patients with TSC and contribute to the elucidation of JXG pathogenesis and treatment.
Costs of illness in amyotrophic lateral sclerosis (ALS): a cross-sectional survey in Germany
Authors: Schönfelder Erik , Osmanovic Alma , Müschen Lars Hendrik , Petri Susanne , Schreiber-Katz Olivia
Published: June 2020

Background

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder. Consequently, patients undergo a multidisciplinary treatment that often requires intensive use of medical resources. This study provides an estimate on the cost of illness depending on the clinical severity while also analysing the patients’ health-related quality of life.

Methods

Primary data from patients and caregivers was collected through a standardised questionnaire. Direct medical, direct non-medical and indirect costs were calculated using the latest German health economic guidelines. Patients were divided into five groups according to the King’s staging system. Health-related quality of life was assessed using EuroQoL Group EQ-5D-5L™ questionnaire. Influencing factors on both total cost and quality of life were examined.

Results

The mean annual total cost of illness was 78,256€ per patient while the lifetime cost per patient was estimated at 246,184€. The prevalence based total burden yearly therefore was 519,776,352€ in Germany. Nearly half of the costs were attributable to informal care. With increase of the clinical severity stage, costs rose and quality of life decreased. The score of the revised Amyotrophic Laterals Sclerosis Functional Rating Scale was identified as one major influencing factor on total costs, while subjective impairment in daily activities and classification into a care level as opposed to having no care level influenced patients’ quality of life.

Conclusion

It is essential to understand the socioeconomic burden of a disease. These data can be used to improve patient care standards and quality of life while also serving as a basis for cost-benefit analyses during the approval process of new treatments.
Effects of nusinersen after one year of treatment in 123 children with SMA type 1 or 2: a French real-life observational study
Authors: Audic Frédérique , de la Banda Marta Gomez Garcia , Bernoux Delphine , Ramirez-Garcia Paola , Durigneux Julien , Barnerias Christine , Isapof Arnaud , Cuisset Jean-Marie , Cances Claude , Richelme Christian , Vuillerot Carole , Laugel Vincent , Ropars Juliette , Altuzarra Cécilia , Espil-Taris Caroline , Walther-Louvier Ulrike , Sabouraud Pascal , Chouchane Mondher , Vanhulle Catherine , Trommsdorff Valérie , Pervillé Anne , Testard Hervé , Lagrue Emmanuelle , Sarret Catherine , Avice Anne-Laude , Beze-Beyrie Pierre , Pauly Vanessa , Quijano-Roy Susana , Chabrol Brigitte , Desguerre Isabelle
Published: June 2020

Background

Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord. Nusinersen has been covered by public healthcare in France since May 2017. The aim of this article is to report results after 1 year of treatment with intrathecal nusinersen in children with SMA types 1 and 2 in France. Comparisons between treatment onset (T0) and after 1 year of treatment (Y1) were made in terms of motor function and need for nutritional and ventilatory support. Motor development milestone achievements were evaluated using the modified Hammersmith Infant Neurologic Examination–Part 2 (HINE-2) for patients under 2 years of age and Motor Function Measure (MFM) scores for patients over 2 years of age.

Results

Data on 204 SMA patients (type 1 or 2) were retrospectively collected from the 23 French centers for neuromuscular diseases. One hundred and twenty three patients had been treated for at least 1 year and were included, 34 of whom were classified as type 1 (10 as type 1a/b and 24 as type 1c) and 89 as type 2. Survival motor Neuron 2 (SMN2) copy numbers were available for all but 6 patients.

Patients under 2 years of age (n = 30), had significantly higher HINE-2 scores at year 1 than at treatment onset but used more nutritional and ventilatory support. The 68 patients over 2 years of age evaluated with the Motor Function Measure test had significantly higher overall scores after 1 year, indicating that their motor function had improved. The scores were higher in the axial and proximal motor function (D2) and distal motor function (D3) parts of the MFM scale, but there was no significant difference for standing and transfer scores (D1). No child in either of the two groups achieved walking.

Conclusion

Nusinersen offers life-changing benefits for children with SMA, particularly those with more severe forms of the disorder. Caregiver assessments are positive. Nevertheless, patients remain severely disabled and still require intensive support care. This new treatment raises new ethical challenges.
Ultrasonographic documentation of type-3 abomasal ulcer in a cow with left displacement of the abomasum
Authors: Gerspach Christian , Oschlies Carina , Kuratli Jasmin , Braun Ueli
Published: June 2020

Background

Ultrasonographic documentation of perforated abomasal ulcer has not been published till now. This report describes the clinical, ultrasonographic and postmortem findings in a Jersey cow with type-3 abomasal ulcer and left displacement of the abomasum (LDA).

Case report

The main clinical findings were abnormal demeanour, rumen atony and tympany, positive foreign body tests, positive auscultation and simultaneous ballottement on the left side, abdominal guarding and loss of negative pressure in the abdominal cavity. The tentative diagnosis was peritonitis and LDA. Abdominal ultrasonography produced images typical of LDA, and in one location between the abdominal wall and abomasum there was a layer of fibrin, a fibrin clot, a break in the abomasal contour, suggestive of a perforated ulcer, and partial obstruction of this gap with fibrin. The diagnosis of perforated abomasal ulcer with subsequent peritonitis was confirmed during postmortem examination.

Conclusions

The examination of this case shows that under certain circumstances, ultrasonographic imaging of a perforated abomasal ulcer in a cow is possible. Antemortem diagnosis of type-3 abomasal ulcer is preferable to relying on exploratory laparotomy and/or post-mortem examination.
Correction to: Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting
Authors: Abasalizadeh Farhad , Moghaddam Sevil Vaghefi , Alizadeh Effat , Akbari Elahe , Kashani Elmira , Fazljou Seyyed Mohammad Bagher , Torbati Mohammadali , Akbarzadeh Abolfazl
Published: June 2020

An amendment to this paper has been published and can be accessed via the original article.
The BDNF-FoxO1 Axis in the medial prefrontal cortex modulates depressive-like behaviors induced by chronic unpredictable stress in postpartum female mice
Authors: Liu Jing , Meng Fantao , Dai Juanjuan , Wu Min , Wang Wentao , Liu Cuilan , Zhao Di , Wang Hongcai , Zhang Jingyan , Li Chen
Published: June 2020

Postpartum depression (PPD) is a serious psychiatric disorder, affecting not only the childbearing women but also the health of their offsprings. The brain-derived neurotrophic factor (Bdnf) gene is an important target gene for the study of depression and antidepressant therapy. FoxO1, belonging to the FoxO subfamily is involved in the development of major depressive disorders. However, the role of BDNF and its functional brain regions involved in PPD remains unknown. Here, we report that chronic unpredictable stress (CUS) can produce depression-associated behaviors in postpartum female mice. CUS can decrease totalBdnfmRNA and exon specific mRNAs in the medial prefrontal cortex (mPFC), accompanied by reduced protein levels, that were correlated with depression-related behaviors. Moreover, postpartum, not virgin female mice showed increased susceptibility to subthreshold stress-induced depression-related behaviors. Selective deletion of BDNF in the mPFC induced anhedonia as indicated by reduced sucrose preference and increased latency to food in the novelty suppressed food test in postpartum, but not in virgin female mice. Furthermore, we found that FoxO1 is also decreased in CUS-treated postpartum female mice with a significant correlation with depression-related behaviors. BDNF-specific knockout in the mPFC decreased FoxO1 expression in female mice. Our results indicate that the BDNF-FoxO1 axis in mPFC can regulate depression-related behaviors and stress vulnerability in postpartum female mice.
Extended doxycycline treatment versus salpingectomy in the management of patients with hydrosalpinx undergoing IVF-ET
Authors: Fouda Usama M. , Elshaer Hesham S. , Youssef Mohamed A. , Darweesh Fatma F.
Published: June 2020

Background

The aim of this study was to determine whether the treatment with doxycycline before and after oocyte retrieval is as effective as salpingectomy in minimizing the detrimental effect of hydrosalpinx on the outcomes of IVF-ET.

Methods

A retrospective analysis was done for the outcomes of the IVF-ET cycles of patients with hydrosalpinx who underwent laparoscopic salpingectomy prior to IVF cycle (n = 260) or were treated with extended doxycycline treatment during the IVF cycle (n = 45). In doxycycline group, doxycycline (100 mg twice daily) was started 1 week before anticipated oocyte retrieval and was continued for 1 week after oocyte retrieval. In salpingectomy group, the mesosalpinx was coagulated as close as possible to the fallopian tube.

Results

The implantation, clinical pregnancy, ongoing pregnancy and live birth rates were significantly higher in the salpingectomy group (20.87% Vs. 9.91%, P value =0.007, 44.62% Vs. 20%, P value = 0.002, 39.62% Vs. 17.78%, P value = 0.005 and 37.31% Vs. 15.56%, P value = 0.005 respectively).

Conclusion

Salpingectomy is more effective than extended doxycycline treatment in improving the outcomes of IVF-ET in patients with hydrosalpinx undergoing IVF-ET. Further, larger well designed randomized controlled trials should be conducted to confirm the findings of this study.
The interaction between arterial oxygenation and carbon dioxide and hospital mortality following out of hospital cardiac arrest: a cohort study
Authors: McGuigan Peter J. , Shankar-Hari Manu , Harrison David A. , Laffey John G. , McAuley Danny F.
Published: June 2020

Background

Outcomes following out of hospital cardiac arrest (OHCA) are poor. The optimal arterial oxygen and carbon dioxide (PaCO2) levels for managing patients following OHCA are unknown. We hypothesized that abnormalities in arterial oxygenation (PaO2/FiO2ratio or PaO2) and PaCO2would be associated with hospital mortality following OHCA. We hypothesized that PaCO2would significantly modify the oxygenation–mortality relationship.

Methods

This was an observational cohort study using data from OHCA survivors admitted to adult critical care units in England, Wales and Northern Ireland from 2011 to 2018. Logistic regression analyses were performed to assess the relationship between hospital mortality and oxygenation and PaCO2.

Results

The analysis included 23,625 patients. In comparison with patients with a PaO2/FiO2 > 300 mmHg, those with a PaO2/FiO2 ≤ 100 mmHg had higher mortality (adjusted OR, 1.79; 95% CI, 1.48 to 2.15;P < 0.001). In comparison to hyperoxemia (PaO2 > 100 mmHg), patients with hypoxemia (PaO2 < 60 mmHg) had higher mortality (adjusted OR, 1.34; 95% CI, 1.10 to 1.65;P = 0.004). In comparison with normocapnia, hypercapnia was associated with lower mortality. Hypocapnia (PaCO2 ≤ 35 mmHg) was associated with higher mortality (adjusted OR, 1.91; 95% CI, 1.63 to 2.24;P < 0.001). PaCO2modified the PaO2/FiO2–mortality and PaO2–mortality relationships, though these relationships were complex. Patients who were both hyperoxic and hypercapnic had the lowest mortality.

Conclusions

Low PaO2/FiO2ratio, hypoxemia and hypocapnia are associated with higher mortality following OHCA. PaCO2modifies the relationship between oxygenation and mortality following OHCA; future studies examining this interaction are required.
An outbreak of bovine babesiosis in February, 2019, triggered by above average winter temperatures in southern England and co-infection with Babesia divergens and Anaplasma phagocytophilum
Authors: Johnson Nicholas , Paul Phipps L. , McFadzean Harriet , Barlow Alex M.
Published: June 2020

Background

Bovine babesiosis, commonly known as redwater fever, is a sporadic tick-borne disease in the United Kingdom. Outbreaks occur during the spring, summer and autumn months when ticks are active. This study reports the findings of an investigation of an outbreak of bovine babesiosis during the winter month of February, 2019.

Methods

DNA from blood, organ and tick samples taken from affected cattle were tested for the presence of piroplasm and Anaplasma phagocytophilum DNA using PCRs directed to the 18S rRNA gene and msp2 gene respectively. The species of piroplasm was confirmed by sequencing.

Results

Babesia divergens DNA was detected in the blood of five cattle displaying clinical signs of babesiosis within a herd of twenty. This parasite was also detected in three of ten ticks removed from one of the affected cattle. In addition, A. phagocytophilum was detected in three cattle tested and two of ten of the ticks.

Conclusions

An outbreak of bovine babesiosis during February is unusual as the tick vector, Ixodes ricinus, does not generally become active until temperatures rise later in the year. February of 2019 was unusual as average temperatures during the first week of the month reached over 10 °C, well above historical averages that are typically below 5 °C, and a temperature at which ticks can become active. This unusual weather event is likely to have triggered tick questing, that combined with a co-infection with two tick-borne pathogens caused the severe outbreak of disease.
First epidemiological survey of Angiostrongylus vasorum in domestic dogs from Spain
Authors: Carretón Elena , Morchón Rodrigo , Falcón-Cordón Yaiza , Matos Jorge , Costa-Rodríguez Noelia , Montoya-Alonso José A.
Published: June 2020

Background

Angiostrongylus vasorumis the causative agent of canine angiostrongylosis, a disease that mainly affects domestic dogs and other wild carnivores. In Europe, the number of infected individuals is increasing, being located in central and southern countries. In Spain, several studies have reported high prevalence ofA. vasorumin wild animals. However, there are no studies addressing the current situation of the disease or its distribution in domestic dogs, and reports from veterinary personnel are very limited. Considering these facts, the objective of the present study was to evaluate the prevalence ofA. vasorumin different areas of Spain.

Methods

Between November 2018 and October 2019, blood was sampled from a total of 2024 domestic dogs from six zones of Spain with a climate that favours the establishment of the disease, where all dogs included in the study lived outdoors or had regular access to areas with vegetation and none had travelled outside the study area of interest in the past year. Details about their sex and age were collected. All dogs were tested for the presence ofA. vasorumcirculating antigens using Angio DetectTM.

Results

The overall prevalence of canine angiostrongylosis in the studied areas of Spain was 1.73%. No differences in overall prevalence were found between males and females, neither between age groups. Regarding eco-epidemiological areas, the highest prevalence was recorded in the zones located in the north and northwest of Spain (1.86–2.74%), which correspond to the wetter climates and most abundant vegetation, and the lowest prevalence was detected in the zones located in the center and west of Spain (0.93–0.99%).

Conclusions

Our data suggest that angiostrongylosis is present in Spain in domestic dogs where previously infected wild animals existed or where climatic conditions are favourable for the establishment of the disease.
Low food and nutrition literacy (FNLIT): a barrier to dietary diversity and nutrient adequacy in school age children
Authors: Doustmohammadian Azam , Omidvar Nasrin , Keshavarz-Mohammadi Nastaran , Eini-Zinab Hassan , Amini Maryam , Abdollahi Morteza , Amirhamidi Zeinab , Haidari Homa
Published: June 2020

Objective

This study aims to assess the relationship between Food and Nutrition Literacy (FNLIT) and dietary diversity score (DDS); FNLIT and nutrient adequacy (NAR%, MAR%) in school-age children in Iran.

Results

This cross-sectional study was undertaken on 803 primary school students in Tehran, Iran. Socio-economic, as well as three 24-h dietary recalls were collected through interviewing students and their mothers/caregivers. FNLIT was measured by a self-administered locally designed and validated questionnaire. Low level of FFNL was significantly associated with higher odds of low DDS (OR = 2.19, 95% CI 1.32–3.62), the first tertile of fruit diversity score (OR = 3.88, 95% CI 2.14–6.99), and the first tertile of dairy diversity score (OR = 9.60, 95% CI 2.07–44.58). Low level of IFNL was significantly associated with probability of lower meat diversity score (OR = 1.73, 95% CI 1.07–2.81). Low level of FLL was also significantly associated with probability of lower DDS (OR = 1.81, 95% CI 1.11–2.94), dairy diversity score (OR = 2.01, 95% CI 1.02–3.98), and meat diversity score (OR = 2.14, 95% CI 1.32–3.45).Low FNLIT and its subscales were associated with higher odds of low level of NAR of protein, calcium, vitamin B3, B6, B9, as well as the probability of lower level of MAR.
Infinitely many positive solutions for a nonlocal problem with competing potentials
Authors: Yang Jing
Published: June 2020

The present paper deals with a class of nonlocal problems. Under some suitable assumptions on the decay rate of the coefficients, we derive the existence of infinitely many positive solutions to the problem by applying reduction method. Comparing to the previous work, we encounter some new challenges because of competing potentials. By doing some delicate estimates for the competing potentials, we overcome the difficulties and find infinitely many positive solutions.
Correction: GM604 regulates developmental neurogenesis pathways and the expression of genes associated with amyotrophic lateral sclerosis
Authors: Swindell William R. , Bojanowski Krzysztof , Kindy Mark S. , Chau Raymond M. W. , Ko Dorothy
Published: June 2020

An amendment to this paper has been published and can be accessed via the original article.
Application of machine learning procedures for mechanical system modelling: capabilities and caveats to prediction-accuracy
Authors: Groensfelder Thomas , Giebeler Fabian , Geupel Marco , Schneider David , Jaeger Rebecca
Published: June 2020

This article presents an investigation about prediction accuracy of multi-parametric models derived from numerical data. Three different mechanical test-cases are used for the generation of the numerical data. From this data, models are derived for the prediction of characteristic variation to arbitrary changes of the input parameters. Different modeling approaches are evaluated regarding their prediction accuracy. Polynomial matrix equations are compared to regression models and neural network models provided by Machine-Learning toolboxes. Similarities and differences of the models are worked out. An exponential matrix-equation-model is proposed to increase accuracy for certain applications. Influences and their causes to the prediction accuracy for the model predictions are evaluated. From this minimum requirements for deriving valuable models are defined. Leading to a comparison of the modelling approaches in relation to physical plausibility and model efficiency. Where efficiency is related to the effort for data creation and training-procedure. For one of the sample cases, a prediction-model is applied to demonstrate the model application and capabilities. The model equation is used to calculate the value of a penalty function in a multi-input/multi-output optimization task. As outcome of the optimization, four natural frequencies are fitted to measured values by updating material parameters. For all other cases sensitivity-studies including verification to numerical results are conducted.
Child passenger safety education in the emergency department: teen driving, car seats, booster seats, and more
Authors: Smola Cassi , Sorrentino Annalise , Shah Nipam , Nichols Michele , Monroe Kathy
Published: June 2020

Background

The leading cause of death in children less than 19 years old is motor vehicle crashes (MVC). Non-use or improper use of motor vehicle car seats significantly adds to the morbidity and mortality. Emergency department (ED) encounters provide an opportunity for caregiver education. Our objective was to determine the effect of an educational intervention on knowledge and counseling behaviors of pediatric ED nurses regarding child passenger safety (CPS).

Methods

A pre/post educational intervention study was conducted with nursing staff in an urban ED. Responses to CPS related knowledge and counseling behaviors were collected using surveys administered before and after the intervention. The ED nurse education intervention was a one-hour lecture based on the American Academy of Pediatrics (AAP) CPS guidelines and Alabama state law regarding ages for each car seat type and teen driving risky behaviors. Individual data from pre and post surveys were matched, and nominal variables in pre-post matched pairs were analyzed using McNemar’s test. To compare categorical variables within pre or post test data, we used the Chi-square test.

Results

Pretests were administered to 83/110 ED nurses; 64 nurses received the educational intervention and posttest. On the pretests, nurses reported “never” or “occasionally” counseling about CPS for the following: 56% car seats, 62% booster seat, 56% teen driving, 32% seat belts. When comparing the pretest CPS knowledge between nurses working 0-1 year vs. ≥ 2 years there was no statistically significant difference. Two CPS knowledge questions did not show significance due to a high correct baseline knowledge rate (> 98%), including baseline knowledge of MVC being the leading cause of death. Of the remaining 7 knowledge questions, 5 questions showed statistically significant improvement in knowledge: age when children can sit in front seat, state GDL law details, seat belt state law for back seat riders, age for booster seat, and rear facing car seat age. All four counseling behavior questions showed increases in intent to counsel families; however, only intent to counsel regarding teen driving reached statistical significance.

Conclusions

Educational efforts improved pediatric ED nursing knowledge regarding CPS. Intent to counsel was also improved following the education.
Expansion of a multi-pronged safe sleep quality improvement initiative to three children’s hospital campuses
Authors: Leong Traci , Roome Kerryn , Miller Terri , Gorbatkin Olivia , Singleton Lori , Agarwal Maneesha , Lazarus Sarah Gard
Published: June 2020

Background

The American Academy of Pediatrics (AAP) recommends infants should be Alone, on their Back, and in a clear Crib to combat relatively stagnant rates of sudden unexpected infant death (SUID). These are referred to as the ABCs of safe sleep. Studies have shown these recommendations are not consistently followed in the hospital setting, but further investigation would determine how to improve the rate of adherence. The objective of this study was to evaluate the impact of an expanded safe sleep initiative at three Georgia free-standing children’s hospital campuses before and after a multipronged safe sleep initiative.

Methods

A quality improvement program with a pre/post analysis was performed using a convenience method of sampling. Infants < 12 months old in three inpatient pediatric campuses were analyzed pre- and post- interventions. The intervention included: 1) nursing education, 2) identification of nurse “safe sleep” champions, 3) crib cards, 4) crib audits, and 5) weekly reporting of data showing nursing unit ABC compliance via tracking boards. The goal was ABC compliance of ≥25% for the post-intervention period. A standardized crib audit tool evaluated sleep position/location, sleep environment, and ABC compliance (both safe position/location and environment). Chi square analysis, Fisher’s exact test, and logistic regression were used to compare safe sleep behaviors before and after the interventions.

Results

There were 204 cribs included pre-intervention and 274 cribs post-intervention. Overall, there was not a significant change in sleep position/location (78.4 to 76.6%, p = 0.64). There was a significant increase in the percent of infants sleeping in a safe sleep environment following the intervention (5.9 to 39.8%, p < 0.01). Overall ABC compliance, including both sleep position/location and environment, improved from 4.4% pre-intervention to 32.5% post-intervention (p < 0.01). There was no significant variability between the hospitals (p = 0.71, p = 1.00).

Conclusions

The AAP’s safe sleep recommendations are currently not upheld in children’s hospitals, but safer sleep was achieved across three children’s campuses in this study. Significant improvements were made in sleep environment and overall safe sleep compliance with this multi-pronged initiative.
Dynamic precise orbit determination of Hayabusa2 using laser altimeter (LIDAR) and image tracking data sets
Authors: Yamamoto Keiko , Otsubo Toshimichi , Matsumoto Koji , Noda Hirotomo , Namiki Noriyuki , Takeuchi Hiroshi , Ikeda Hitoshi , Yoshikawa Makoto , Yamamoto Yukio , Senshu Hiroki , Mizuno Takahide , Hirata Naru , Yamada Ryuhei , Ishihara Yoshiaki , Araki Hiroshi , Abe Shinsuke , Yoshida Fumi , Higuchi Arika , Sasaki Sho , Oshigami Shoko , Tsuruta Seiitsu , Asari Kazuyoshi , Shizugami Makoto , Ogawa Naoko , Ono Go , Mimasu Yuya , Yoshikawa Kent , Takahashi Tadateru , Takei Yuto , Fujii Atsushi , Yamaguchi Tomohiro , Kikuchi Shota , Watanabe Sei-ichiro , Tanaka Satoshi , Terui Fuyuto , Nakazawa Satoru , Saiki Takanao , Tsuda Yuichi
Published: June 2020

The precise orbit of the Hayabusa2 spacecraft with respect to asteroid Ryugu is dynamically determined using the data sets collected by the spacecraft’s onboard laser altimeter (LIght Detection And Ranging, LIDAR) and automated image tracking (AIT). The LIDAR range data and the AIT angular data play complementary roles because LIDAR is sensitive to the line-of-sight direction from Hayabusa2 to Ryugu, while the AIT is sensitive to the directions perpendicular to it. Using LIDAR and AIT, all six components of the initial state vector can be derived stably, which is difficult to achieve using only LIDAR or AIT. The coefficient of solar radiation pressure (SRP) of the Hayabusa2 spacecraft and standard gravitational parameter (GM) of Ryugu can also be estimated in the orbit determination process, by combining multiple orbit arcs at various altitudes. In the process of orbit determination, the Ryugu-fixed coordinate of the center of the LIDAR spot is determined by fitting the range data geometrically to the topography of Ryugu using the Markov Chain Monte Carlo method. Such an approach is effective for realizing the rapid convergence of the solution. The root mean squares of the residuals of the observed minus computed values of the range and brightness-centroid direction of the image are 1.36 m and 0.0270°, respectively. The estimated values of the GM of Ryugu and a correction factor to our initial SRP model are 29.8 ± 0.3 m3/s2 and 1.13 ± 0.16, respectively.