bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Profiled Unsupervised Horizontal Iterative Attack against Hardware Elliptic Curve Scalar Multiplication Using Machine Learning
Authors: Aftowicz Marcin , Kabin Ievgen , Dyka Zoya , Langendörfer Peter
Published: January 2024

While IoT technology makes industries, cities, and homes smarter, it also opens the door to security risks. With the right equipment and physical access to the devices, the attacker can leverage side-channel information, like timing, power consumption, or electromagnetic emanation, to compromise cryptographic operations and extract the secret key. This work presents a side channel analysis of a cryptographic hardware accelerator for the Elliptic Curve Scalar Multiplication operation, implemented in a Field-Programmable Gate Array and as an Application-Specific Integrated Circuit. The presented framework consists of initial key extraction using a state-of-the-art statistical horizontal attack and is followed by regularized Artificial Neural Networks, which take, as input, the partially incorrect key guesses from the horizontal attack and correct them iteratively. The initial correctness of the horizontal attack, measured as the fraction of correctly extracted bits of the secret key, was improved from 75% to 98% by applying the iterative learning.
A Systematic Comparative Study on the Physicochemical Properties, Volatile Compounds, and Biological Activity of Typical Fermented Soy Foods
Authors: Guo Qingyan , Peng Jiabao , He Yujie
Published: January 2024

Fermented soy foods can effectively improve the unpleasant odor of soybean and reduce its anti-nutritional factors while forming aromatic and bioactive compounds. However, a differential analysis of characteristic flavor and function among different fermented soy foods has yet to be conducted. In this study, a systematic comparison of different fermented soy foods was performed using E-nose, HS-SMPE-GC×GC-MS, bioactivity validation, and correlation analysis. The results showed that soy sauce and natto flavor profiles significantly differed from other products. Esters and alcohols were the main volatile substances in furu, broad bean paste, douchi, doujiang, and soy sauce, while pyrazine substances were mainly present in natto. Phenylacetaldehyde contributed to the sweet aroma of furu, while 1-octene-3-ol played a crucial role in the flavor formation of broad bean paste. 2,3-Butanediol and ethyl phenylacetate contributed fruity and honey-like aromas to douchi, doujiang, and soy sauce, respectively, while benzaldehyde played a vital role in the flavor synthesis of douchi. All six fermented soy foods demonstrated favorable antioxidative and antibacterial activities, although their efficacy varied significantly. This study lays the foundation for elucidating the mechanisms of flavor and functionality formation in fermented soy foods, which will help in the targeted development and optimization of these products.
Hydrogel Extrusion Speed Measurements for the Optimization of Bioprinting Parameters
Authors: Arjoca Stelian , Bojin Florina , Neagu Monica , Păunescu Andreea , Neagu Adrian , Păunescu Virgil
Published: January 2024

Three-dimensional (3D) bioprinting is the use of computer-controlled transfer processes for assembling bioinks (cell clusters or materials loaded with cells) into structures of prescribed 3D organization. The correct bioprinting parameters ensure a fast and accurate bioink deposition without exposing the cells to harsh conditions. This study seeks to optimize pneumatic extrusion-based bioprinting based on hydrogel flow rate and extrusion speed measurements. We measured the rate of the hydrogel flow through a cylindrical nozzle and used non-Newtonian hydrodynamics to fit the results. From the videos of free-hanging hydrogel strands delivered from a stationary print head, we inferred the extrusion speed, defined as the speed of advancement of newly formed strands. Then, we relied on volume conservation to evaluate the extrudate swell ratio. The theoretical analysis enabled us to compute the extrusion speed for pressures not tested experimentally as well as the printing speed needed to deposit hydrogel filaments of a given diameter. Finally, the proposed methodology was tested experimentally by analyzing the morphology of triple-layered square-grid hydrogel constructs printed at various applied pressures while the printing speeds matched the corresponding extrusion speeds. Taken together, the results of this study suggest that preliminary measurements and theoretical analyses can simplify the search for the optimal bioprinting parameters.
The Intriguing Mystery of RPA Phosphorylation in DNA Double-Strand Break Repair
Authors: Fousek-Schuller Valerie , Borgstahl Gloria
Published: January 2024

Human Replication Protein A (RPA) was historically discovered as one of the six components needed to reconstitute simian virus 40 DNA replication from purified components. RPA is now known to be involved in all DNA metabolism pathways that involve single-stranded DNA (ssDNA). Heterotrimeric RPA comprises several domains connected by flexible linkers and is heavily regulated by post-translational modifications (PTMs). The structure of RPA has been challenging to obtain. Various structural methods have been applied, but a complete understanding of RPA’s flexible structure, its function, and how it is regulated by PTMs has yet to be obtained. This review will summarize recent literature concerning how RPA is phosphorylated in the cell cycle, the structural analysis of RPA, DNA and protein interactions involving RPA, and how PTMs regulate RPA activity and complex formation in double-strand break repair. There are many holes in our understanding of this research area. We will conclude with perspectives for future research on how RPA PTMs control double-strand break repair in the cell cycle.
Extra Virgin Olive Oil Phenolic Compounds Modulate the Gene Expression of Biomarkers Involved in Fibroblast Proliferation and Differentiation
Authors: González-Acedo Anabel , Illescas-Montes Rebeca , de Luna-Bertos Elvira , Ruiz Concepción , Ramos-Torrecillas Javier , García-Martínez Olga , Melguizo-Rodríguez Lucía
Published: January 2024

Extra virgin olive oil phenolic compounds have been identified as possible biostimulant agents against different pathological processes, including alterations in healing processes. However, there is little evidence on the molecular mechanisms involved in this process. The aim was to analyse the effect of hydroxytyrosol, tyrosol, and oleocanthal on fibroblast gene expression. PCR was used to determine the expression of different differentiation markers, extracellular matrix elements, and growth factors in cultured human fibroblasts CCD-1064Sk treated with different doses of hydroxytyrosol (10−5 M and 10−6 M), tyrosol (10−5 M and 10−6 M), and oleocanthal (10−6 M and 10−7 M). After 24 h of hydroxytyrosol treatment, increased expression of connective tissue growth factor, fibroblast growth factor (FGF), platelet-derived growth factor, vascular endothelial growth factor, transforming growth factor β1 (TGF-β1), and their receptors was observed. Tyrosol and olecanthal modulated the expression of FGF and TGFβR1. All phytochemicals tested modified the expression of differentiation markers and extracellular matrix elements, increasing gene expression of actin, fibronectin, decorin, collagen I, and III. Phenolic compounds present in extra virgin olive could have a beneficial effect on tissue regeneration by modulating fibroblast physiology.
Research Progress of Group II Intron Splicing Factors in Land Plant Mitochondria
Authors: Li Xiulan , Jiang Yueshui
Published: January 2024

Mitochondria are important organelles that provide energy for the life of cells. Group II introns are usually found in the mitochondrial genes of land plants. Correct splicing of group II introns is critical to mitochondrial gene expression, mitochondrial biological function, and plant growth and development. Ancestral group II introns are self-splicing ribozymes that can catalyze their own removal from pre-RNAs, while group II introns in land plant mitochondria went through degenerations in RNA structures, and thus they lost the ability to self-splice. Instead, splicing of these introns in the mitochondria of land plants is promoted by nuclear- and mitochondrial-encoded proteins. Many proteins involved in mitochondrial group II intron splicing have been characterized in land plants to date. Here, we present a summary of research progress on mitochondrial group II intron splicing in land plants, with a major focus on protein splicing factors and their probable functions on the splicing of mitochondrial group II introns.
Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂
Authors: Zhao Yun , Zhou Junming , Dong Yanzhen , Xu Dayong , Qi Dongming
Published: January 2024

Artificial hybrid breeding can optimize parental traits to cultivate excellent hybrids with enhanced economic value. In this study, we investigated the growth performance and transcriptomes of Gymnocypris przewalskii (♀) and Gymnocypris eckloni (♂) and their F1 hybrid fishes. Hatched individuals of G. przewalskii (GP) and G. eckloni (GE) of the same size and their F1 hybrids (GH) were separately cultured for eight months in three cement tanks (n = 3). The growth indexes were measured, which showed that the growth rate of the groups was GE > GH > GP, while the survival rate was GH > GE > GP. The RNA-Seq data analysis of the muscles from the three Gymnocypris fish strains revealed that gene transcription has a significant impact on F1 hybrid fish and its parents. The differentially expressed genes (DEGs) in GH show less differences with GP, but more with GE. qRT-PCR was used to confirm the expression profiles of the chosen DEGs, and the results showed positive correlations with the RNA-seq data. KEGG enrichment results indicated that the DEGs were related to a variety of molecular functions, such as glycolysis/gluconeogenesis, arachidonic acid formation, citrate cycle, and the MAPK, PI3K-Akt, or mTOR signal pathways. Subsequent analysis indicated that there may be a significant correlation between the differential expression of IGF2 and a difference in the growth of GE and GP.
Optimization of Structural Design in Steel Buildings Based on the Site-Specific Design Spectra of the Mexico Seismic Regulations
Authors: Ramos-Sánchez Juan Antonio , Díaz Sergio A. , Mora-Ortiz René Sebastián , Polanco-Sotomayor Gabriel , Magaña-Hernández Francisco
Published: January 2024

Seismic risk management in urban areas requires accurate prediction of the expected seismic hazard. The seismic design standards in the world provide the seismic design spectra (DS). These are crucial for estimating seismic forces on a structure, are typically derived from theoretical models in deterministic or probabilistic seismic hazard studies, especially for bedrock soils. Characterizing soil dynamic amplification frequencies or periods is necessary to establish site-specific design spectra (DSsite). Geotechnical and geophysical studies, along with environmental vibration records, determine soil stratigraphy characteristic features and their dominant frequency or period. These parameters improve our understanding of seismic wave behavior from bedrock to surface soil during earthquakes. This article details the utilization of geotechnical, geophysical studies, and environmental vibration records to estimate DSsite in accordance with Mexican seismic regulation and examines the cost–benefit aspects of using the Dssite in optimizing the structural design of a medium-rise steel building in southeastern Mexico, characterized by soft soil and a moderate seismic hazard. The case study demonstrates an 18% cost savings in the structural elements of the building by employing the DSsite with more rational spectral ordinates for study site.
Antifungal and Antioomycete Activities of a Curcuma longa L. Hydroethanolic Extract Rich in Bisabolene Sesquiterpenoids
Authors: Cruz Adriana , Sánchez-Hernández Eva , Teixeira Ana , Martín-Ramos Pablo , Cunha Ana , Oliveira Rui
Published: January 2024

Curcuma longa, known for its anti-inflammatory, antioxidant, and antimicrobial properties, has been a staple in traditional medicine for centuries. In the pursuit of natural alternatives to synthetic preservatives, the extracts of C. longa have garnered attention for their efficacy in extending shelf life and preventing the spoilage of diverse agricultural products. This study aims to assess the antifungal and antioomycete activities and plant protection capabilities of a hydroethanolic C. longa extract as a natural product against crop pathogens. The phytochemical profile of the C. longa extract was elucidated through gas chromatography-mass spectrometry. The extract exhibited a richness in bisabolene sesquiterpenoids, notably (+)-β-turmerone, α-turmerone, (+)-(S)-ar-turmerone, and included minor phytoconstituents, such as α-atlantone, γ-curcumene, zingiberene, isoelemicin, and gibberellin A3. Radial growth inhibition assays demonstrated the C. longa extract’s effectiveness against various phytopathogenic fungi, including Botrytis cinerea, Colletotrichum acutatum, and Diplodia corticola, as well as the oomycetes Phytophthora cactorum and Phytophthora cinnamomi. In ex situ tests, the C. longa extract demonstrated remarkable protection capabilities for Malus domestica excised stems against P. cinnamomi-induced necrosis. Furthermore, the C. longa extract exhibited non-toxicity towards lettuce seed germination and did not impact early lettuce seedling growth, indicating its safety for crop protection. These findings contribute to expanding the potential applications of C. longa as an antimicrobial agent, particularly for safeguarding economically significant trees against the destructive oomycete P. cinnamomi.
Assessment of Beef Manure Economic Value by the Method of Equivalent Green and Mineral Fertilizer Substitution
Authors: Šarac Veljko , Milić Dragan , Vukelić Nataša , Novaković Tihomir , Novaković Dragana , Ljubojević Mirjana , Rodić Vesna
Published: January 2024

The imperative of sustainable agricultural development, coupled with growing challenges related to climate change reaffirms the importance of manure and increases the demand for it. Due to the underdeveloped market for manure, there is a problem in assessing its economic value, requiring appropriate research on this topic. Thus, this research aimed to assess the manure’s economic value using the method of equivalent substitution. For this purpose, the chemical composition of manure was determined by standard agrochemical analyses concerning the content of the most important mineral elements—nitrogen (N), phosphorus (P2O5), and potassium (K2O). These elements ranged from 0.49–0.60%, 0.15–1.10%, and 0.70–1.07%, respectively, and the content of organic matter ranging from 20.88 to 27.00%. Subsequently, the cost of equivalent substitution was calculated taking into account market prices for commercial fertilizers and organic matter based on the cost of an adequate quantity of white mustard—Sinapis alba L. fresh mass. The average cost of equivalent substitution for manure was determined to be 28.60 USD/t. The results of the applied t-test indicate that there is no statistically significant difference between the market prices of beef manure and the calculated prices of its substitution (t = −1.4069; df = 12; p = 0.1848), which implies that white mustard green manure could replace the deficit in animal-derived manure. Since both animal and green manures unambiguously should be prioritized over chemical fertilizers, future studies will reveal which other crops are applicable for further increments of green manure to make up for insufficient availability of animal manure.
Examining an Alternate Care Pathway for Mental Health and Addiction Prehospital Emergencies in Ontario, Canada: A Critical Analysis
Authors: Meijer Petra , Ford-Jones Polly , Carter Dustin , Duhaney Patrina , Adam Simon , Pomeroy Danielle , Thompson Sheryl
Published: January 2024

Paramedics in Ontario have largely been limited to transporting those with mental health or addiction (MHA)-related emergencies to the emergency department (ED). The ED has repeatedly been identified as a problematic and challenging setting for people with MHA needs. This article examines an innovative patient care model (PCM) established by the Middlesex–London Paramedic Service and its partners for specific MHA emergencies where patients were given options for care that included transportation to a Canadian Mental Health Association (CMHA) Crisis Centre or information for support. Qualitative and quantitative data that were utilized for regular reporting to the Ministry were included in the analysis. The findings indicated that the goals of reducing pressures on EDs and paramedic services, enhancing paramedics’ ability to address MHA calls, and improving patient care experiences were met. This model improves patient autonomy and options for care, improves the means for addressing patients’ social determinants of health, and offers transportation to a non-medicalized facility.
Piloting an Alternative Implementation Modality for a School-Based Child Sexual Abuse Prevention Curriculum
Authors: Mozid Nusrat E , Espinosa Rebeca N. , Grayson Corinne , Falode Oluwatumininu , Yang Yilei , Glaudin Christelle , Guastaferro Kate
Published: January 2024

Background: In the U.S., the most pervasive child sexual abuse (CSA) prevention strategy involves school-based prevention programs; however, the reach of these programs is limited due to implementation constraints, such as budgets or turnover. This is notable as standard delivery of often requires two facilitators in the classroom. Leveraging a natural experiment in the implementation of Safe Touches, the current study sought to explore the feasibility of implementation with a single facilitator using pre-recorded videos compared to the standard in-person delivery. Methods: A six-item CSA-related knowledge questionnaire was delivered to (N = 1480) second-graders post-workshop. An independent-samples t-test was used to compare the mean of CSA-related knowledge item responses for each delivery modality. Student-level data were paired with teacher evaluations and an interview with the facilitator. Results: Across workshops delivered in 25 schools, there was no significant difference in knowledge based on CSA-related questions by workshop modality. Teachers indicated the facilitators responded effectively to the children’s questions and comments in both delivery modalities. Input from the facilitator was positive. Conclusions: Triangulation of student knowledge, teacher input, and facilitator experience indicates the viability and feasibility of this implementation strategy for Safe Touches, and potentially other school-based CSA prevention programs. To ensure equitable access to the CSA prevention program, the empirical examination of, and investment in, alternative implementation options for school-based CSA preventive programs is encouraged.
Cognitive Impairment and Non-Communicable Diseases in Egypt’s Aging Population: Insights and Implications from the 2021–2022 Pilot of “A Longitudinal Study of Egyptian Healthy Aging” “AL-SEHA”
Authors: Moustafa Sara A. , Deif Reem , Gaballah Nada , Salama Mohamed
Published: January 2024

As the global population ages, the prevalence of cognitive impairment among older individuals has been steadily rising. Like many countries, Egypt is grappling with the challenges an aging demographic poses. The global network of longitudinal aging studies, modeled after the US Health and Retirement Study (HRS), includes over 40 countries but lacks representation from the Arab/North African region. The proposed ‘A Longitudinal Study of Egyptian Healthy Aging’ (AL-SEHA) will address this gap by providing data on aging in Egypt, the largest Arab/North African country, shedding light on the intricate relationship between cognitive impairment and non-communicable diseases (NCDs) in Egypt’s aging population between 2021 and 2022. This study took place in five governments in Egypt and recruited 299 participants from a population of 50+. The results of the study are from the pilot stage of the original longitudinal study (AL-SEHA).
Enhancing the K-Means Algorithm through a Genetic Algorithm Based on Survey and Social Media Tourism Objectives for Tourism Path Recommendations
Authors: Damos Mohamed A. , Zhu Jun , Li Weilian , Khalifa Elhadi , Hassan Abubakr , Elhabob Rashad , Hm Alaa , Ei Esra
Published: January 2024

Social media platforms play a vital role in determining valuable tourist objectives, which greatly aids in optimizing tourist path planning. As data classification and analysis methods have advanced, machine learning (ML) algorithms such as the k-means algorithm have emerged as powerful tools for sorting through data collected from social media platforms. However, traditional k-means algorithms have drawbacks, including challenges in determining initial seed values. This paper presents a novel approach to enhance the k-means algorithm based on survey and social media tourism data for tourism path recommendations. The main contribution of this paper is enhancing the traditional k-means algorithm by employing the genetic algorithm (GA) to determine the number of clusters (k), select the initial seeds, and recommend the best tourism path based on social media tourism data. The GA enhances the k-means algorithm by using a binary string to represent initial centers and to apply GA operators. To assess its effectiveness, we applied this approach to recommend the optimal tourism path in the Red Sea State, Sudan. The results clearly indicate the superiority of our approach, with an algorithm optimization time of 0.01 s. In contrast, traditional k-means and hierarchical cluster algorithms required 0.27 and 0.7 s, respectively.
Epigenetic Methylation Changes in Pregnant Women: Bisphenol Exposure and Atopic Dermatitis
Authors: Kim Seung Hwan , Yu So Yeon , Choo Jeong Hyeop , Kim Jihyun , Ahn Kangmo , Hwang Seung Yong
Published: January 2024

Bisphenol is a chemical substance widely used in plastic products and food containers. In this study, we observed a relationship between DNA methylation and atopic dermatitis (AD) in the peripheral blood mononuclear cells (PBMCs) of pregnant women exposed to bisphenol A (BPA) and its alternatives, bisphenol S (BPS) and bisphenol F (BPF). DNA methylation is an epigenetic mechanism that regulates gene expression, which can be altered by environmental factors, and affects the onset and progression of diseases. We found that genes belonging to the JAK-STAT and PI3K-AKT signaling pathways were hypomethylated in the blood of pregnant women exposed to bisphenols. These genes play important roles in skin barrier function and immune responses, and may influence AD. Therefore, we suggest that not only BPA, but also BPS and BPF, which are used as alternatives, can have a negative impact on AD through epigenetic mechanisms.
Performance Measures and Plasma Biomarker Levels in Patients with Multiple Sclerosis after 14 Days of Fampridine Treatment: An Explorative Study
Authors: Thorning Maria , Lambertsen Kate Lykke , Jensen Henrik Boye , Frich Lars Henrik , Madsen Jonna Skov , Olsen Dorte Aalund , Holsgaard-Larsen Anders , Nielsen Helle Hvilsted
Published: January 2024

Peripheral cytokine levels may serve as biomarkers for treatment response and disease monitoring in patients with multiple sclerosis (pwMS). The objectives were to assess changes in plasma biomarkers in PwMS after 14 days of fampridine treatment and to explore correlations between changes in performance measures and plasma biomarkers. We included 27 PwMS, 14 women and 13 men, aged 52.0 ± 11.6 years, with a disease duration of 17 ± 8.5 years, and an Expanded Disability Status Scale of 6 [IQR 5.0/6.5]. Gait and hand function were assessed using performance tests completed prior to fampridine and after 14 days of treatment. Venous blood was obtained, and chemiluminescence analysis conducted to assess plasma cytokines and neurodegenerative markers. All performance measures demonstrated improvements. Biomarkers showed decreased tumor necrosis factor (TNF) receptor-2 levels. Associations were found between change scores in (i) Six Spot Step Test and Interleukin (IL)-2, IL-8, and IL-17 levels; (ii) timed 25-foot walk and interferon-γ, IL-2, IL-8, TNF-α, and neurofilament light levels, and (iii) 12-Item Multiple Sclerosis Walking Scale and IL-17 levels. The associations may reflect increased MS-related inflammatory activity rather than a fampridine-induced response or that a higher level of inflammation induces a better response to fampridine.
The Anti-Inflammatory Potential of an Ethanolic Extract from Sarcopoterium spinosum Fruits for Protection and/or Counteraction against Oxidative Stress in Dysfunctional Endothelial Cells
Authors: Zbeeb Hawraa , Baldini Francesca , Zeaiter Lama , Vergani Laura
Published: January 2024

Plants and plant extracts are a relevant source of bioactive compounds widely employed as functional foods. In the Mediterranean area, the shrub Sarcopoterium spinosum is traditionally used as an herbal medicine for weight loss and a diabetes treatment. Inflammation is a protective mechanism involved in the development of many pathological conditions, including cardiovascular diseases. The present study aimed to investigate in vitro the antioxidant and cytoprotective properties of an ethanolic extract from S. spinosum fruits (SEE) in a cellular model of endothelium dysfunction. Corilagin and quercetin are two polyphenols abundant in SEE and were tested for comparison. The exposure of HECV cells for 24 h to 30 µM hydrogen peroxide (H2O2) lead to an oxidative stress condition. When HECV cells were treated with 10 µg/mL of SEE or single compounds after or before the oxidative insult, the results showed their ability to (i) decrease the reactive oxygen species (ROS) production quantified using fluorometric analysis and the lipid peroxidation measured with a spectrophotometric assay; (ii) rescue both the glutathione reduced to oxidized (GSH/GSSG) ratio and nitric oxide impair and the protein denaturation; and (iii) accelerate the wound repair measured using a T-scratch assay. Taken together, our findings indicate that the ethanolic extract from S. spinosum fruits could be a potential candidate for nutraceutical application.
Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
Authors: Olmo Rocío , Quijada Narciso M. , Morán-Diez María Eugenia , Hermosa Rosa , Monte Enrique
Published: January 2024

The tomato (Solanum lycopersicum) is an important crop worldwide and is considered a model plant to study stress responses. Small RNAs (sRNAs), 21–24 nucleotides in length, are recognized as a conserved mechanism for regulating gene expression in eukaryotes. Plant endogenous sRNAs, such as microRNA (miRNA), have been involved in disease resistance. High-throughput RNA sequencing was used to analyze the miRNA profile of the aerial part of 30-day-old tomato plants after the application of the fungus Trichoderma atroviride to the seeds at the transcriptional memory state. Compared to control plants, ten differentially expressed (DE) miRNAs were identified in those inoculated with Trichoderma, five upregulated and five downregulated, of which seven were known (miR166a, miR398-3p, miR408, miR5300, miR6024, miR6027-5p, and miR9471b-3p), and three were putatively novel (novel miR257, novel miR275, and novel miR1767). miRNA expression levels were assessed using real-time quantitative PCR analysis. A plant sRNA target analysis of the DE miRNAs predicted 945 potential target genes, most of them being downregulated (84%). The analysis of KEGG metabolic pathways showed that most of the targets harbored functions associated with plant–pathogen interaction, membrane trafficking, and protein kinases. Expression changes of tomato miRNAs caused by Trichoderma are linked to plant defense responses and appear to have long-lasting effects.
Topical Skin Application of Small-Molecule Antiplatelet Agent against Pressure Injury in Rat Models
Authors: Yuan Yuan , Takashi En , Hou Ping , Kamijo Akio , Miura Daiji , Ten Hirotomo
Published: January 2024

Due to prolonged forced positioning, the incidence of intraoperative pressure injuries is high. This study aimed to explore the impact of small-molecule antiplatelet drugs on pressure injuries by locally applying them before an injury occurs. In the first part of this study, water-soluble tracers with different molecular weights were applied to normal and early-stage pressure-injured skin. Through digital cameras, spectrophotometers, and histological observations, the penetration of tracers into the epidermis was clarified. In the second part of this study, a water-soluble antiplatelet drug called Trapidil (molecular weight = 205 Da) was applied to the left side of the back of a rat before, during, and after compression, and the contralateral side served as a non-intervention control group. The differences in pressure injuries between the two groups were observed through a digital camera, an ultraviolet camera, and temperature measurement, and skin circulation and perfusion were assessed via an intravenous injection of Evans Blue. The first part of this study found that water-soluble tracers did not easily penetrate normal skin but could more easily penetrate pressure-damaged skin. The smaller the molecular weight of the tracer, the easier it penetrated the skin. Therefore, in the next step of research, water-soluble drugs with smaller molecular weights should be selected. The second part of this study found that, compared with the control group, the occurrence rates and areas of ulcers were lower, the gray value was higher, and the skin temperature was lower in the Trapidil group (p < 0.05). After the intravenous Evans Blue injection, skin circulation and perfusion in the Trapidil group were found to be better. In conclusion, this study found that the topical skin application of a small-molecule antiplatelet agent may have significant effects against pressure injuries by improving post-decompression ischemia, providing new insights into the prevention and treatment of intraoperative pressure injuries.
Involvement of the Na+, K+-ATPase α1 Isoform and Endogenous Cardiac Steroids in Depression- and Manic-like Behaviors
Authors: Horesh Noa , Pelov Ilana , Pogodin Ilana , Zannadeh Hiba , Rosen Haim , Mikhrina Anastasiia Leonidovna , Dvela-Levitt Moran , Sampath Vishnu Priya , Lichtstein David
Published: January 2024

Bipolar disorder (BD) is a severe and common chronic mental illness characterized by recurrent mood swings between depression and mania. The biological basis of the disease is poorly understood, and its treatment is unsatisfactory. Na+, K+-ATPase is a major plasma membrane transporter and signal transducer. The catalytic α subunit of this enzyme is the binding site for cardiac steroids. Three α isoforms of the Na+, K+-ATPase are present in the brain. Previous studies have supported the involvement of the Na+, K+-ATPase and endogenous cardiac steroids (ECS) in the etiology of BD. Decreased brain ECS has been found to elicit anti-manic and anti-depressive-like behaviors in mice and rats. However, the identity of the specific α isoform involved in these behavioral effects is unknown. Here, we demonstrated that decreasing ECS through intracerebroventricular (i.c.v.) administration of anti-ouabain antibodies (anti-Ou-Ab) decreased the activity of α1+/− mice in forced swimming tests but did not change the activity in wild type (wt) mice. This treatment also affected exploratory and anxiety behaviors in α1+/− but not wt mice, as measured in open field tests. The i.c.v. administration of anti-Ou-Ab decreased brain ECS and increased brain Na+, K+-ATPase activity in wt and α1+/− mice. The serum ECS was lower in α1+/− than wt mice. In addition, a study in human participants demonstrated that serum ECS significantly decreased after treatment. These results suggest that the Na+, K+-ATPase α1 isoform is involved in depressive- and manic-like behaviors and support that the Na+, K+-ATPase/ECS system participates in the etiology of BD.
The Genus Cladosporium: A Prospective Producer of Natural Products
Authors: Li Yanjing , Wang Yifei , Wang Han , Shi Ting , Wang Bo
Published: January 2024

Cladosporium, a genus of ascomycete fungi in the Dematiaceae family, is primarily recognized as a widespread environmental saprotrophic fungus or plant endophyte. Further research has shown that the genus is distributed in various environments, particularly in marine ecosystems, such as coral reefs, mangroves and the polar region. Cladosporium, especially the marine-derived Cladosporium, is a highly resourceful group of fungi whose natural products have garnered attention due to their diverse chemical structures and biological activities, as well as their potential as sources of novel leads to compounds for drug production. This review covers the sources, distribution, bioactivities, biosynthesis and structural characteristics of compounds isolated from Cladosporium in the period between January 2000 and December 2022, and conducts a comparative analysis of the Cladosporium isolated compounds derived from marine and terrestrial sources. Our results reveal that 34% of Cladosporium-derived natural products are reported for the first time. And 71.79% of the first reported compounds were isolated from marine-derived Cladosporium. Cladosporium-derived compounds exhibit diverse skeletal chemical structures, concentrating in the categories of polyketides (48.47%), alkaloids (19.21%), steroids and terpenoids (17.03%). Over half of the natural products isolated from Cladosporium have been found to have various biological activities, including cytotoxic, antibacterial, antiviral, antifungal and enzyme-inhibitory activities. These findings testify to the tremendous potential of Cladosporium, especially the marine-derived Cladosporium, to yield novel bioactive natural products, providing a structural foundation for the development of new drugs.
Characterization of a Novel N4-Methylcytosine Restriction-Modification System in Deinococcus radiodurans
Authors: Shi Chenxiang , Wang Liangyan , Xu Hong , Zhao Ye , Tian Bing , Hua Yuejin
Published: January 2024

Deinococcus radiodurans is an extremophilic microorganism that possesses a unique DNA damage repair system, conferring a strong resistance to radiation, desiccation, oxidative stress, and chemical damage. Recently, we discovered that D. radiodurans possesses an N4-methylation (m4C) methyltransferase called M.DraR1, which recognizes the 5′-CCGCGG-3′ sequence and methylates the second cytosine. Here, we revealed its cognate restriction endonuclease R.DraR1 and recognized that it is the only endonuclease specially for non-4C-methylated 5′-CCGCGG-3′ sequence so far. We designated the particular m4C R.DraR1-M.DraR1 as the DraI R-M system. Bioinformatics searches displayed the rarity of the DraI R-M homologous system. Meanwhile, recombination and transformation efficiency experiments demonstrated the important role of the DraI R-M system in response to oxidative stress. In addition, in vitro activity experiments showed that R.DraR1 could exceptionally cleave DNA substrates with a m5C-methlated 5′-CCGCGG-3′ sequence instead of its routine activity, suggesting that this particular R-M component possesses a broader substrate choice. Furthermore, an imbalance of the DraI R-M system led to cell death through regulating genes involved in the maintenance of cell survival such as genome stability, transporter, and energy production. Thus, our research revealed a novel m4C R-M system that plays key roles in maintaining cell viability and defending foreign DNA in D. radiodurans.
Review on the Application of the Attention Mechanism in Sensing Information Processing for Dynamic Welding Processes
Authors: Xu Jingyuan , Liu Qiang , Xu Yuqing , Xiao Runquan , Hou Zhen , Chen Shanben
Published: January 2024

Arc welding is the common method used in traditional welding, which constitutes the majority of total welding production. The traditional manual and manual teaching welding method has problems with high labor costs and limited efficiency when faced with mass production. With the advancement in technology, intelligent welding technology is expected to become a solution to this problem in the future. To achieve the intelligent welding process, modern sensing technology can be employed to effectively simulate the welder’s sensory perception and cognitive abilities. Recent studies have advanced the application of sensing technologies, leading to the advancement in intelligent welding process. The review is divided into two aspects. First, the theory and applications of various sensing technologies (visual, sound, arc, spectral signal, etc.) are summarized. Then, combined with the generalization of neural networks and attention mechanisms, the development trends in welding sensing information processing and modeling technology are discussed. Based on the existing research results, the feasibility, advantages, and development direction of attention mechanisms in the welding field are analyzed. In the end, a brief conclusion and remarks are presented.
The Ecology of Non-Candida Yeasts and Dimorphic Fungi in Cetaceans: From Pathogenicity to Environmental and Global Health Implications
Authors: Garcia-Bustos Victor , Acosta-Hernández Begoña , Cabañero-Navalón Marta Dafne , Pemán Javier , Ruiz-Gaitán Alba Cecilia , Rosario Medina Inmaculada
Published: January 2024

Cetaceans, which are integral to marine ecosystems, face escalating anthropogenic threats, including climate change and pollution, positioning them as critical sentinel species for ocean and human health. This review explores the neglected realm of non-Candida yeasts in cetaceans, addressing the gaps in the understanding of their prevalence, pathogenicity, and environmental impacts. By examining identified species such as Cryptococcus spp., Paracoccidioides spp., and several dimorphic fungi, this review emphasizes global prevalence, epidemiology and ecology, pathogenicity, and potential zoonotic implications. It also discusses the fine line between yeast commensalism and pathogenicity by considering environmental influences such as pollution, climate shifts, and immune suppression. Environmental impact discussions delve into how rising ocean temperatures and pollution can modify yeast mycobiota, potentially affecting marine host health and broader ecosystem dynamics. The cetacean’s unique physiology and ecological niches are considered, highlighting potential impacts on behaviors, reproductive success, and survival rates. Identifying crucial knowledge gaps, the review calls for intensified research efforts, employing advanced molecular techniques to unravel the cetacean mycobiome. Systematic studies on yeast diversity, antifungal susceptibility, and their influence on environmental and ecosystem health are proposed, and the balance between commensal and pathogenic species emphasizes the significance of the One Health approach. In conclusion, as marine mammals face unprecedented challenges, unveiling non-Candida yeasts in cetaceans emerges as a critical endeavor with far-reaching implications for the conservation of marine ecosystems and for both animal and human public health.
Pins Gene Table v2.0: An Online Genome Database of 37 Pythium insidiosum Strains for Gene Content Exploration and Phylogenomic Analysis
Authors: Kittichotirat Weerayuth , Patumcharoenpol Preecha , Rujirawat Thidarat , Tangphatsornruang Sithichoke , Yurayart Chompoonek , Krajaejun Theerapong
Published: January 2024

Unlike most pathogenic oomycetes, Pythium insidiosum infects humans and animals instead of plants. P. insidiosum has three clinically relevant genotypes/clades that cause a severe disease called pythiosis. To develop strategies for infection control, it is necessary to understand the biology and pathogenesis of this pathogen. Investigating the evolutionary mechanisms behind the host-specific adaptation is vital, and comparative genomic analysis can help with this. To facilitate genomic analysis, an online bioinformatics tool called P. insidiosum (Pins) Gene Table v2.0 was developed. This tool includes genomic data from 37 genetically diverse P. insidiosum strains and four related species. The database contains 732,686 genes, grouped into 80,061 unique clusters and further divided into core and variable categories at genus, species, and genotype levels. A high-resolution phylogenomic relationship among P. insidiosum strains and other oomycetes was projected through hierarchical clustering and core gene analyses. 3156 P. insidiosum-specific genes were shared among all genotypes and may be responsible for causing disease in humans and animals. After comparing these species-specific genes to the MvirDB database, 112 had significant matches with 66 known virulence proteins, some of which might be involved in vascular occlusion, which is a pathological feature of pythiosis. The correlation of genotypes, geographic origins, and affected hosts of P. insidiosum suggests that clade-I strains are more specific to animals, while clade-II/III strains are more specific to humans. The clade-specific genes might link to host preference. In summary, Pins Gene Table v2.0 is a comprehensive genome database accessible to users with minimal bioinformatics experience for the analysis of P. insidiosum genomes.
Freehand Technique for Pedicle Screw Placement during Surgery for Adolescent Idiopathic Scoliosis Is Associated with Less Ionizing Radiation Compared to Intraoperative Navigation
Authors: Obid Peter , Zahnreich Sebastian , Frodl Andreas , Rahim Tamim , Niemeyer Thomas , Mayr Moritz
Published: January 2024

Purpose: We aim to compare radiation exposure and implant-related complications of the freehand (FH) technique versus intraoperative image-guided navigation (IN) for pedicle screw placement in adolescent idiopathic scoliosis (AIS) and estimate associated lifetime attributable cancer risks. Methods: A retrospective analysis of prospectively collected data from 40 consecutive AIS patients treated with pedicle screw instrumentation using the FH technique was performed. The dose area product (DAP) and effective dose (ED) were calculated. Screw-related complications were analysed, and the age- and gender-specific lifetime attributable cancer risks were estimated. The results were compared to previously published data on IN used during surgery for AIS. Results: There were no implant-related complications in our cohort. Implant density was 86.6%. The mean Cobb angle of the main curve was 75.2° (SD ± 17.7) preoperatively and 27.7° (SD ± 10.8) postoperatively. The mean ED of our cohort and published data for the FH technique was significantly lower compared to published data on the IN technique (p < 0.001). The risk for radiogenic cancer for our FH technique AIS cohort was 0.0014% for male patients and 0.0029% for female patients. Corresponding risks for IN were significantly higher (p < 0.001), ranging from 0.0071 to 0.124% and from 0.0144 to 0.253% for male and female patients, respectively. Conclusion: The routine use of intraoperative navigation in AIS surgery does not necessarily reduce implant-related complications but may increase radiation exposure to the patient.
Complementizer Agreement and the Licensing of DPs: An Account in Terms of Referential Anchoring
Authors: Hinterhölzl Roland
Published: January 2024

In this paper, I argue that the phenomenon of complementizer agreement in West Germanic and the distribution of DPs in German can be given a common explanation in terms of an approach in which context values are not freely assigned via an interpretive function operation, as is assumed in standard accounts of formal semantics, but rather, they become accessible in a specific functional head in the C-domain.
Chemical Analysis and Biological Activities of Extracts Isolated from Symbiotic L. japonicus Plants
Authors: Kalousi Foteini D. , Tsakos Michail , Nikolaou Christina N. , Georgantopoulos Achilleas , Psarra Anna-Maria G. , Tsikou Daniela
Published: January 2024

Plants produce a wide variety of secondary metabolites, including compounds with biological activities that could be used for the treatment of human diseases. In the present study, we examined the putative production of bioactive molecules in the legume plant Lotus japonicus, which engages into symbiotic relationships with beneficial soil microorganisms. To monitor the production of secondary metabolites when the plant develops beneficial symbiotic relationships, we performed single and double inoculations with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing Rhizobium bacteria. Plant extracts from non-inoculated and inoculated plants were chemically characterized and tested for anti-proliferative, apoptotic, and anti-inflammatory effects on human HEK-293 cells. Both shoot and root extracts from non-inoculated and inoculated plants significantly reduced the HEK-293 cell viability; however, a stronger effect was observed when the root extracts were tested. Shoot and root extracts from Rhizobium-inoculated plants and shoot extracts from AMF-inoculated plants showed apoptotic effects on human cells. Moreover, both shoot and root extracts from AMF-inoculated plants significantly reduced TNFα-induced NF-κB transcriptional activity, denoting anti-inflammatory activity. These results suggest that symbiotic L. japonicus plants are enriched with metabolites that have interesting biological activities and could be further explored for putative future use in the pharmaceutical sector.
Tirbanibulin 1% Ointment for Actinic Keratosis: Results from a Real-Life Study
Authors: Li Pomi Federica , Vaccaro Mario , Pallio Giovanni , Rottura Michelangelo , Irrera Natasha , Borgia Francesco
Published: January 2024

Background and Objectives: Tirbanibulin 1% ointment is a novel synthetic anti-proliferative agent that inhibits tubulin polymerization. It is approved for treating actinic keratosis (AK) on the face and scalp in adults. It has demonstrated good efficacy, an adequate safety profile and excellent patient adherence in the phase 3 clinical trials, however data about its real-life efficacy and safety are lacking. Here we report the experience of the dermatology unit of the University Hospital of Messina. Materials and Methods: We performed a spontaneous open-label, prospective non-randomized study to assess the effectiveness and safety of tirbanibulin 1% ointment for the treatment of 228 AKs in 38 consecutive patients—28 males (73%) and 10 females (26%)—aged between 52 and 92 years (mean age: 72 ± 8.92 years). Results: Total clearance was recorded in 51% of lesions, while partial clearance was recorded in 73% of lesions. An excellent tolerability profile and high compliance rate were observed, with no treatment discontinuation due to the onset of adverse events. Conclusion: Our real-life experience confirms the effectiveness and safety of tirbanibulin ointment for the treatment of AKs.