bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Sow Maternal Behavior on the Growth of Piglets and a Genome-Wide Association Study
Authors: Liu Xin , Li Hanmei , Wang Ligang , Zhang Longchao , Wang Lixian
Published: December 2023

Sows’ maternal behavior is important for improving piglet survival and growth; thus, breeding for good mothering sows is necessary for pig production. However, there is little research on the genetic mechanism of maternal behavior. In this study, a comparative analysis of piglets’ growth traits between good and bad maternal behavior groups and a genome-wide association study (GWAS) was performed to elucidate the impact of sows’ maternal behavior on piglet growth and identify candidate genes and markers of sow’s maternal behaviors. Comparing the growth traits of piglets between good and bad sows’ maternal behavior groups, the results showed that the growth traits of piglets from sows with good maternal behavior were better than those from sows with bad maternal behavior and especially for the multiparous sows group, this comparative difference was significant. For the intensive study of the genetic mechanisms of sows’ maternal behavior, a total of 452 sows were genotyped using the Illumina Porcine 50K SNP Chip, and 4 traits, including biting piglets (BP), crushing piglets (CP), trampling piglets (TP) and screaming test (ST), were examined. Using a GWAS, 20 single nucleotide polymorphisms (SNPs) were found to be associated with these traits. Within 1 Mb upstream and downstream of the significant SNPs screened, 138 genes were obtained. After pathway enrichment and gene annotation, HIP1, FZD9 and HTR7 were identified as important candidate genes affecting sows’ maternal behaviors. These findings preliminarily elucidate the genetic basis of sows’ maternal behavior traits and provide candidate genes and markers for molecular breeding in pigs.
Population Genetic Divergence among Worldwide Gene Pools of the Mediterranean Mussel Mytilus galloprovincialis
Authors: Ouagajjou Yassine , Aghzar Adil , Presa Pablo
Published: December 2023

The Mediterranean mussel Mytilus galloprovincialis is distributed in both hemispheres either natively or introduced. The updated population genetic distribution of this species provides a useful knowledge against which future distribution shifts could be assessed. This study, performed with seven microsatellite markers and three reference species (M. edulis, M. chilensis and M. trossulus), aimed to determine the scenario of genetic divergence between 15 samples of M. galloprovincialis from 10 localities in Europe, Africa, Asia, Australia, North America and South America. In agreement with previous data, M. trossulus was the most divergent taxon of the genus, but M. chilensis appeared as an intermediate taxon between M. edulis and M. galloprovincialis, though closer to this latter. M. galloprovincialis from the Atlantic Northeast appears as the most likely source of worldwide exotic settlements instead of the previously thought Mediterranean population. The successful worldwide establishment of M. galloprovincialis suggests it is a flexible evolutionary species (FES), i.e., a species or population whose genetic background allows it to rapidly adapt to changing environments. This natural endowed plastic adaptation makes it a candidate resilient species amidst the ongoing climatic change.
Impact of In Vitro Digestion on the Digestibility, Amino Acid Release, and Antioxidant Activity of Amaranth (Amaranthus cruentus L.) and Cañihua (Chenopodium pallidicaule Aellen) Proteins in Caco-2 and HepG2 Cells
Authors: Serena-Romero Gloricel , Ignot-Gutiérrez Anaís , Conde-Rivas Osvaldo , Lima-Silva Marlenne Y. , Martínez Armando J. , Guajardo-Flores Daniel , Cruz-Huerta Elvia
Published: December 2023

This study evaluated the impact of in vitro gastrointestinal digestion on the digestibility, amino acid release, and antioxidant activity of proteins from amaranth (Amarantus cruentus L.) and cañihua (Chenopodium pallidicaule Aellen). Antioxidant activity was assessed using ORAC, ABTS, DPPH, and cellular antioxidant activity (CAA) assays in human intestinal Caco-2 and hepatic Hep-G2 cell lines. The results showed that amaranth had higher protein digestibility (79.19%) than cañihua (71.22%). In addition, intestinal digestion promoted the release of essential amino acids, such as leucine, lysine, and phenylalanine, in both protein concentrates. Concentrations of amaranth and cañihua proteins, ranging from 0.125 to 1.0 mg mL−1, were non-cytotoxic in both cell lines. At a concentration of 0.750 mg mL−1, simulated gastrointestinal digestion enhanced cellular antioxidant activity. Intestinal digest fractions containing peptides >5 kDa were the principal contributors to CAA in both cell lines. Notably, cañihua proteins exhibited high CAA, reaching values of 85.55% and 82.57% in Caco-2 and HepG2 cells, respectively, compared to amaranth proteins, which reached 84.68% in Caco-2 and 81.06% in HepG2 cells. In conclusion, both amaranth and cañihua proteins, after simulated gastrointestinal digestion, showcased high digestibility and released peptides and amino acids with potent antioxidant properties, underscoring their potential health benefits.
Optimising Amber Processing Using 3D Scanning: New Perspectives in Cultural Heritage
Authors: Korga Sylwester , Dziedzic Krzysztof , Skulimowski Stanisław , Gnapowski Sebastian
Published: December 2023

This article aims to present the practical and functional application of advanced 3D scanning technologies in the process of designing products made of amber. The processing of amber is complex and expensive because mass or serial production is not possible. Moreover, processing is challenging because each product is treated as unique and requires individual handling. The optimisation process is illustrated using the example of amber blocks that were scanned to obtain virtual 3D models. The scope of this work includes the characterisation of three-dimensional jewellery design processes, along with a description of a wide range of methods and functions used in computer-aided jewellery design. The use of computer-aided design software for developing virtual models of decorative products used in goldsmithing is described in this paper. The obtained sets of models underwent engineering analysis using Boolean algebra and data science calculations. In this way, a design methodology was developed regarding the selection of input material as the unprocessed stone for jewellery modelling. The developed method allows for the minimisation of material waste, which is associated with reducing costs in the processing of amber. This article also describes the differences between previous traditional methods and the authors’ proprietary method. The proposed methodology can be implemented in jewellery workshops specialising in the processing of materials.
Method for Controlling Full-Frequency Band Environment Vibration by Coordinating Metro Vibration Sources and Propagation Paths
Authors: Tan Xinyu , Jiang Bolong , Qi Chunyu , Ma Meng , Liu Jizhao , Hu Wenlin , Wang Shaolin
Published: December 2023

Floating slab tracks (FSTs) are used to reduce the impact of vibration on precision instruments and historical relics along metro lines; however, ground vibration is universally amplified at the natural frequency of the tracks. In this study, a full-frequency control method that considers frequency matching for environmental vibrations, in combination with metro vibration sources and propagation paths, was developed based on the bandgap theory of the periodic structure. The effectiveness of this method was analysed by establishing a three-dimensional metro train–FST coupled model and a finite element analysis model of track bed–tunnel–soil–row piles. The results show that ground vibration can be reduced by approximately 3–5 dB at the natural frequency of the FST by adjusting the bandgap range of the periodic piles to 7–9 Hz, eliminating the adverse effect of vibration amplification at the natural frequency of the FSTs. The proposed control method shows good vibration control effects and can effectively minimise ground vibration in the full-frequency range.
Microbial Diversity of the Produced Waters from the Oilfields in the Republic of Tatarstan (Russian Federation): Participation in Biocorrosion
Authors: Ziganshina Elvira E. , Mohammed Waleed S. , Ziganshin Ayrat M.
Published: December 2023

A variety of microorganisms grow in oil reservoirs, where they participate in the biodegradation of oil and lead to changes in oil quality. Today, our knowledge about microbial processes in oil reservoirs is largely limited, and among the urgent tasks for the oil industry, monitoring and controlling microbial activity (including the activity of microbes responsible for biocorrosion) are important. In this work, we aimed to characterize the bacterial community structure of the produced waters from oilfields in the south of the Republic of Tatarstan (Russian Federation) using cultural and molecular methods of analysis. Bacteria from produced water samples from various oil wells were enriched, and 16S rRNA gene amplicon sequencing was used to assess the phylogenetic diversity of the bacterial communities. Thus, analysis of the bacterial communities revealed the presence of various members within the genera Desulfotomaculum, Clostridium, Acetobacterium, Desulfovibrio, Dethiosulfovibrio, Desulfomicrobium, Fusibacter, Dethiosulfatibacter, Thermovirga, Sphaerochaeta, and Desulfoplanes, but in different produced water samples. The role of the bacterial associations in biocorrosion was separately assessed in experiments on monitoring and stimulating biocorrosion. The bacterial community receiving additional nutrition was shown to have a higher corrosion potential, and scanning electron microscopy analysis confirmed changes in the surface of the metal coupons during immersion testing. The practical value of this research lies in identifying the potential contribution of microbial communities to oil pipeline corrosion.
Underwater Image Super-Resolution via Dual-aware Integrated Network
Authors: Shi Aiye , Ding Haimin
Published: December 2023

Underwater scenes are often affected by issues such as blurred details, color distortion, and low contrast, which are primarily caused by wavelength-dependent light scattering; these factors significantly impact human visual perception. Convolutional neural networks (CNNs) have recently displayed very promising performance in underwater super-resolution (SR). However, the nature of CNN-based methods is local operations, making it difficult to reconstruct rich features. To solve these problems, we present an efficient and lightweight dual-aware integrated network (DAIN) comprising a series of dual-aware enhancement modules (DAEMs) for underwater SR tasks. In particular, DAEMs primarily consist of a multi-scale color correction block (MCCB) and a swin transformer layer (STL). These components work together to incorporate both local and global features, thereby enhancing the quality of image reconstruction. MCCBs can use multiple channels to process the different colors of underwater images to restore the uneven underwater light decay-affected real color and details of the images. The STL captures long-range dependencies and global contextual information, enabling the extraction of neglected features in underwater images. Experimental results demonstrate significant enhancements with a DAIN over conventional SR methods.
Research on Improved Fault Detection Method of Rolling Bearing Based on Signal Feature Fusion Technology
Authors: Fang Zhenggaoyuan , Wu Qing-E , Wang Wenjing , Wu Shuyan
Published: December 2023

Rolling bearings are the core transmission components of large-scale equipment. Once a fault occurs, the consequences may be catastrophic, posing a serious threat to life and the safety of the property. Aimed at the problem of rolling bearing faults, this paper analyzes the characteristics of different fault signals and proposes a fault diagnosis method based on fuzzy signals. Based on the definition of an incomplete mapping of a new connotation, this paper proposes a fuzzy fault diagnosis method by fuzzy mapping, gives a fuzzy signal processing algorithm, and discusses two judgment principles. Further, it carries out a vibration signal analysis of the rolling bearing. According to the fault diagnosis method in this paper, the fault rolling bearing is diagnosed. The experimental results show that the proposed method can effectively diagnose rolling bearing faults closer to their natural attributes and solves the problem of traditional generator bearing fault diagnosis that requires complex models and poor diagnosis speed. Further, it can be seen that the average time consumption of this method is reduced and the fault recognition accuracy rate is increased. Compared with the existing related methods, this proposed diagnosis method is superior to that of several existing methods. It not only has higher precision, stronger anti-noise capacity, and faster diagnosis speed, but also has lower effective information loss.
Modeling and Experimental Verification of the Electrical Efficiency for Variable-Frequency Rolling Piston Compressor under Variable Suction Conditions
Authors: Cheng Zheming , Tao Leren , Huang Lihao , Yu Zhongyang
Published: December 2023

The frequency and the suction refrigerant state are complex in the actual operation of variable compressors, which leads to the variability of compressor performance under different operating conditions. The characterization and the modeling of the compressor have become hot research topics. For modeling, the electrical efficiency model of the compressor, which could be applied to the suction refrigerant state at both the superheated and the two-phase stages, the compressor frequency, the pressure ratio, and the evaporation temperature, is analyzed through experiments in this paper. The results show that the linear electrical efficiency decreases with the declining superheated temperature and the increasing suction vapor quality when the system is at the fixed and evaporating temperature. Moreover, the slope of the two-phase suction is above that of the superheated suction. In addition, the system’s electrical efficiency at the fixed pressure ratio is inversely proportional to the evaporation temperature, and the electrical efficiency of the system at the fixed evaporating temperature is also inversely proportional to the pressure ratio. The higher the variation of the pressure ratio, the smaller the evaporation temperature influences. To demonstrate the precision of the model, the theoretical value is compared with a confirmatory experiment in this paper. The maximum relative error is 1.81%, and the minimum is 0.035%.
Performance Evaluation Model of Landport and Seaport Collaboration Using the Support Vector Machine
Authors: Xu Dehong , Zhang Yue , Liang Chengji
Published: December 2023

This study delves into the pivotal role of shipper satisfaction in cultivating efficacious cooperation between dry ports and seaports. The research endeavors herein present a comprehensive indicator system tailored to evaluate the efficacy of the cooperation of the sea ports and dry ports. This system encompasses 14 key indicators, thoughtfully categorized into four dimensions, offering a holistic perspective on the multifaceted factors that underscore the synergy between dry ports and seaports. The establishment of a performance evaluation model for this collaborative nexus draws upon the support vector machine (SVM) technique, a choice substantiated by its suitability for the available dataset and its relevance within the research context. Leveraging the indicator data as a training dataset, the SVM approach culminates in the construction of a cooperative matrix, ultimately facilitating the discernment of performance rankings within dry port and seaport collaboration. This research methodology not only yields valuable insights into the evaluation of this distinct collaboration but also presents a versatile framework with potential application for addressing various challenges encountered within the cooperative milieu of dry ports and seaports.
The Status of Space Weather Infrastructure and Research in Africa
Authors: Baki Paul , Rabiu Babatunde , Amory-Mazaudier Christine , Fleury Rolland , Cilliers Pierre J. , Adechinan Joseph , Emran Anas , Bounhir Aziza , Cesaroni Claudio , Dinga J. Bienvenue , Doherty Patricia , Gaye Idrissa , Ghalila Hassen , Grodji Franck , Habarulema John-Bosco , Kahindo Bruno , Mahrous Ayman , Messanga Honoré , Mungufeni Patrick , Nava Bruno , Nigussie Melessew , Olwendo Joseph , Sibanda Patrick , Loua René Tato , Uwamahoro Jean , Zaourar Naima , Zerbo Jean-Louis
Published: December 2023

Space weather science has been a growing field in Africa since 2007. This growth in infrastructure and human capital development has been accompanied by the deployment of ground-based observing infrastructure, most of which was donated by foreign institutions or installed and operated by foreign establishments. However, some of this equipment is no longer operational due to several factors, which are examined in this paper. It was observed that there are considerable gaps in ground-based space-weather-observing infrastructure in many African countries, a situation that hampers the data acquisition necessary for space weather research, hence limiting possible development of space weather products and services that could help address socio-economic challenges. This paper presents the current status of space weather science in Africa from the point of view of some key leaders in this field, focusing on infrastructure, situation, human capital development, and the research landscape.
Defining the Role of Metastasis-Initiating Cells in Promoting Carcinogenesis in Ovarian Cancer
Authors: Wang Ji , Ford James C. , Mitra Anirban K.
Published: December 2023

Ovarian cancer is the deadliest gynecological malignancy with a high prevalence of transcoelomic metastasis. Metastasis is a multi-step process and only a small percentage of cancer cells, metastasis-initiating cells (MICs), have the capacity to finally establish metastatic lesions. These MICs maintain a certain level of stemness that allows them to differentiate into other cell types with distinct transcriptomic profiles and swiftly adapt to external stresses. Furthermore, they can coordinate with the microenvironment, through reciprocal interactions, to invade and establish metastases. Therefore, identifying, characterizing, and targeting MICs is a promising strategy to counter the spread of ovarian cancer. In this review, we provided an overview of OC MICs in the context of characterization, identification through cell surface markers, and their interactions with the metastatic niche to promote metastatic colonization.
Sulfated Galactans from Agarophytes: Review of Extraction Methods, Structural Features, and Biological Activities
Authors: Chumsook Khosook , Praiboon Jantana , Fu Xiaoting
Published: December 2023

Agarophytes are important seaweeds of the Rhodophyta type, which have been highly exploited for industrial use as sources of a widely consumed polysaccharide of agar. In addition to that, sulfated galactans (SGs) from agarophytes, which consist of various functional sulfate groups, have attracted the attention of scientists in current studies. SGs possess various biological activities, such as anti-tumor, anticoagulant, anti-inflammatory, antioxidant, anti-obesity, anti-diabetic, anti-microbial, anti-diarrhea, and gut microbiota regulation properties. Meanwhile, the taxonomy, ecological factors, i.e., environmental factors, and harvest period, as well as preparation methods, i.e., the pretreatment, extraction, and purification conditions, have been found to influence the chemical compositions and fine structures of SGs, which have, further, been shown to have an impact on their biological activities. However, the gaps in the knowledge of the properties of SGs due to the above complex factors have hindered their industrial application. The aim of this paper is to collect and systematically review the scientific evidence about SGs and, thus, to pave the way for broader and otherwise valuable industrial applications of agarophytes for human enterprise. In the future, this harvested biomass could be sustainably used not only as a source of agar production but also as natural materials in functional food and pharmaceutical industries.
Blockade of Inflammatory Markers Attenuates Cardiac Remodeling and Fibrosis in Rats with Supravalvular Aortic Stenosis
Authors: Duchatsch Francine , Miotto Danyelle S. , Tardelli Lidieli P. , Dionísio Thiago J. , Campos Dijon S. , Santos Carlos F. , Okoshi Katashi , Amaral Sandra L.
Published: December 2023

Since cardiac inflammation has been considered an important mechanism involved in heart failure, an anti-inflammatory treatment could control cardiac inflammation and mitigate the worsening of cardiac remodeling. This study evaluated the effects of dexamethasone (DEX) and ramipril treatment on inflammation and cardiac fibrosis in an experimental model of heart failure induced by supravalvular aortic stenosis. Wistar rats (21d) were submitted to an aortic stenosis (AS) protocol. After 21 weeks, an echocardiogram and a maximal exercise test were performed, and after 24 weeks, rats were treated with DEX, ramipril or saline for 14d. The left ventricle (LV) was removed for histological and inflammatory marker analyses. The AS group showed exercise intolerance (−32% vs. Sham), higher relative wall thickness (+63%), collagen deposition and capillary rarefaction, followed by cardiac disfunction. Both treatments were effective in reducing cardiac inflammation, but only DEX attenuated the increased relative wall thickness (−17%) and only ramipril reduced LV fibrosis. In conclusion, both DEX and ramipril decreased cardiac inflammatory markers, which probably contributed to the reduced cardiac fibrosis and relative wall thickness; however, treated AS rats did not show any improvement in cardiac function. Despite the complex pharmacological treatment of heart failure, treatment with an anti-inflammatory could delay the patient’s poor prognosis.
Mobile Point-of-Care Device Using Molecularly Imprinted Polymer-Based Chemosensors Targeting Interleukin-1β Biomarker
Authors: Park Rowoon , Jeon Sangheon , Lee Jae Won , Jeong Jeonghwa , Kwon Young Woo , Kim Sung Hyun , Jang Joonkyung , Han Dong-Wook , Hong Suck Won
Published: December 2023

Molecularly imprinted polymers (MIPs) have garnered significant attention as a promising material for engineering specific biological receptors with superior chemical complementarity to target molecules. In this study, we present an electrochemical biosensing platform incorporating MIP films for the selective detection of the interleukin-1β (IL-1β) biomarker, particularly suitable for mobile point-of-care testing (POCT) applications. The IL-1β-imprinted biosensors were composed of poly(eriochrome black T (EBT)), including an interlayer of poly(3,4-ethylene dioxythiophene) and a 4-aminothiophenol monolayer, which were electrochemically polymerized simultaneously with template proteins (i.e., IL-1β) on custom flexible screen-printed carbon electrodes (SPCEs). The architecture of the MIP films was designed to enhance the sensor sensitivity and signal stability. This approach involved a straightforward sequential-electropolymerization process and extraction for leaving behind cavities (i.e., rebinding sites), resulting in the efficient production of MIP-based biosensors capable of molecular recognition for selective IL-1β detection. The electrochemical behaviors were comprehensively investigated using cyclic voltammograms and electrochemical impedance spectroscopy responses to assess the imprinting effect on the MIP films formed on the SPCEs. In line with the current trend in in vitro diagnostic medical devices, our simple and effective MIP-based analytical system integrated with mobile POCT devices offers a promising route to the rapid detection of biomarkers, with particular potential for periodontitis screening.
Parametric Investigation of Self-Centering Prestressed Concrete Frame Structures with Variable Friction Dampers
Authors: Huang Linjie , Qian Zhendong , Meng Yuan , Jiang Kaixi , Zhang Jingru , Sang Chenxu
Published: December 2023

To enhance the structural stiffness and energy-dissipating capacity after the decompression of beam-to-column connections for self-centering prestressed concrete (SCPC) frames, this study presents the seismic performance of a new type of SCPC frame with variable friction dampers (VFDs). The structure is characterized by a third stiffness and a variable energy-dissipating capacity. A 5-story and an 8-story VFD-SCPC frame were selected as the analytical cases, and their numerical models were built based on OpenSees 3.3.0 finite-element software. Sixteen ground-motion records were selected as excitations for the analyses, and the influence of the second stiffness and the third stiffness for the VFD-SCPC connections, as well as the second activation for VFD, on the seismic performance of the structures, was studied. The results showed that increasing the stiffness (number) of prestressed strands and their distance to the center of the beam section can obviously increase the second stiffness of the structures, thus decreasing their displacement, while the distribution mode of inter-story drift along the building’s height cannot be changed. Increasing the third stiffness of the connections (the angle of slope sliding parts and the stiffness for the combination of disc springs) can effectively reduce the deformation of the structures under MCE (maximum-considered earthquakes) seismic levels and improve the energy-dissipation capacity of structures significantly. The premature secondary activation of VFD can enhance the loading capacity and energy-dissipation capacity of structures under both DBE (design-basis earthquakes) and MCE seismic levels, and reduce the inter-story drift of structures effectively.
Analysis of the Impact of Layout Mode on the Wind Environment of Dormitories in Coastal Universities in Southern Fujian Province
Authors: Wu Zhigang , Li Benli , Shi Feng , Xiao Zehong , Hong Xinchen
Published: December 2023

The building wind environment in coastal areas is highly complex and variable, particularly during winter when strong winds prevail. Understanding the influence of winter winds on human comfort and addressing health issues caused by wind noise in human settlements are very valuable research topics. This study focuses on developing strategies to optimize the wind environment in dormitory buildings located in the southern coastal area, where prolonged periods of strong winds have a significant impact. Specifically, we investigated the current wind in the teacher–student dormitory at Jinjiang Campus of Fuzhou University through questionnaire surveys and field measurements. Additionally, computational fluid dynamics (CFD) simulations were employed to evaluate different dormitory layouts. The research results indicate that it is preferable to position the dormitory areas downwind of large-scale buildings, adopt a staggered layout instead of a regular determinant layout to mitigate the narrow tube effect, avoid designing large-scale squares in the center of the dormitory areas, and enclose the dormitory groups with low-level corridors. These findings provide valuable guidance for the optimizing of winter windproof designs of university buildings located in the same climatic zone along the southeast coast.
Dual-Level Framework for OpenBIM-Enabled Design Collaboration
Authors: Jin Ming , Li Baizhan
Published: December 2023

Design coordination and collaboration are crucial in the architecture, engineering, and construction (AEC) industries, necessitating the integration of diverse disciplines and expertise to achieve unified and functional designs. Traditionally, these disciplines operate in isolation, leading to a fragmented design process. Building Information Modeling (BIM), recognized for its collaborative capabilities, presents an opportunity to revolutionize traditional design practices. However, existing research on BIM primarily assumes an ideal environment where all major participants use BIM models, overlooking the dynamics of mixed 2D Computer-aided Design (CAD) and 3D BIM environments. Addressing this research gap, this study aims to establish a dual-level OpenBIM-enabled collaborative design framework, enhancing the design process across various disciplines. The study employs a case study approach, applying this framework to an airport project in Chengdu, China. The airport includes different corridors that are similar in scope and scale but distinct enough to allow for a comparative study. The results demonstrate a 27% faster completion rate, a 98% reduction in design errors, and improved user satisfaction with the proposed method. The paper concludes by discussing the limitations of the study and suggesting avenues for future research.
Changes of C, H, and N Elements of Corn Straw during the Microwave Heating Process
Authors: Liu Zhihong , Cao Weitao , Zhang Man , Zhao Wenke , Zhang Yaning
Published: December 2023

Due to the rapid growth of the global economy, energy consumption has been steadily increasing, leading to increasing issues such as energy shortages and environmental concerns. Biomass energy, a critical renewable energy source, plays a vital role in advancing low-carbon energy development and resource sustainability. In this study, experiments were conducted to study the migration of C, H, and N elements of corn straw during the microwave heating process, and the effects of residence time, heating temperature, and microwave power were also investigated. The results showed that when the temperature rose, both the proportion of C and H elements fluctuated slightly. Specifically, when the temperature rose from 75 °C to 275 °C, there was a 1.02% increase in the proportion of the C element and a 0.25% decrease in the proportion of the H element. Residence time appeared to be a significant factor influencing the changes in C, H, and N elements. For a 40 min residence time, the proportion of the C element increased from 31.77% to 35.36%, while the proportion of the H element decreased from 4.50% to 3.83%. When there was an increase in the microwave power between 160 W and 200 W, higher temperatures were reached in the samples, leading to the carbonization process of corn straw being more complete. Consequently, the proportion of the C element rose with extended residence time, whereas the proportion of the H element decreased as the residence time increased.
Impact of Modern Low Dose Involved Site Radiation Therapy on Normal Tissue Toxicity in Cervicothoracic Non-Hodgkin Lymphomas: A Biophysical Study
Authors: Roers Julian , Rolf Daniel , Baehr Andrea , Pöttgen Christoph , Stickan-Verfürth Martina , Siats Jan , Hering Dominik A. , Moustakis Christos , Grohmann Maximilian , Oertel Michael , Haverkamp Uwe , Stuschke Martin , Timmermann Beate , Eich Hans T. , Reinartz Gabriele
Published: December 2023

This biophysical study aimed to determine fitting parameters for the Lyman–Kutcher–Burman (LKB) dose–response model for normal tissue complication probability (NTCP) calculations of acute side effects and to investigate the impact of reduced radiation doses on the probability of their occurrence in supradiaphragmatic non-Hodgkin lymphoma (NHL) irradiation. A cohort of 114 patients with NHL in the cervicothoracic region, treated between 2015 and 2021 at the University Hospitals of Münster, Hamburg, and Essen, with involved site radiation therapy (ISRT), were included. Among them, 68 patients with aggressive NHL (a-NHL) received consolidative radiation therapy with 24–54 Gy following (R-)CHOP chemotherapy. Additionally, 46 patients with indolent NHL (i-NHL) underwent radiotherapy with 22.5–45.0 Gy. Two treatment plans were prospectively created for each patient (a-NHL: 30.0/40.0 Gy; i-NHL: 24.0/30.0 Gy). NTCP were then calculated using the optimized LKB model. The adapted dose–response models properly predicted the patient’s probability of developing acute side effects when receiving doses ≤ 50 Gy. In addition, it was shown that reduced radiation doses can influence the NTCP of acute side effects depending on the aggressiveness of NHL significantly. This study provided a foundation to prospectively assess the probability of adverse side effects among today’s reduced radiation doses in the treatment of NHL.
Non-Intrinsic, Systemic Mechanisms of Cellular Senescence
Authors: Schwartz Rachael E. , Conboy Irina M.
Published: December 2023

Cellular senescence is believed to contribute to aging and disease through the activity of secreted factors that promote inflammation, remodel the extracellular matrix, and adversely modify the behavior of non-senescent cells. While the markers and properties of senescent cells are still under investigation, it is postulated that cellular senescence manifests in vivo as the consequence of cellular damage that accumulates and becomes exacerbated with time. Yet, the notions that senescence has a solely intrinsic and time-dependent nature are questioned by the rapid induction of senescence in young mice and young cells in vitro by exposure to blood from aged animals. Here, we review some of the research on the systemically present factors that increase with age and may contribute to extrinsically induced senescence or “bystander senescence”. These include proteins, reactive oxygen species, lipids, and nucleic acids, which may be present in individual soluble form, in vesicles, and in non-membranous multi-component macromolecules.
Corticotropin-Releasing Hormone: A Novel Stimulator of Somatolactin in Teleost Pituitary Cells
Authors: Du Ruixin , Shi Xuetao , Chen Feng , Wang Li , Liang Hongwei , Hu Guangfu
Published: December 2023

Corticotropin-releasing hormone (CRH) is known for its crucial role in the stress response system, which could induce pituitary adrenocorticotropic hormone (ACTH) secretion to promote glucocorticoid release in the adrenal gland. However, little is known about other pituitary actions of CRH in teleosts. Somatolactin is a fish-specific hormone released from the neurointermediate lobe (NIL) of the posterior pituitary. A previous study has reported that ACTH was also located in the pituitary NIL region. Interestingly, our present study found that CRH could significantly induce two somatolactin isoforms’ (SLα and SLβ) secretion and synthesis in primary cultured grass carp pituitary cells. Pharmacological analysis further demonstrated that CRH-induced pituitary somatolactin expression was mediated by the AC/cAMP/PKA, PLC/IP3/PKC, and Ca2+/CaM/CaMK-II pathways. Finally, transcriptomic analysis showed that both SLα and SLβ should play an important role in the regulation of lipid metabolism in primary cultured hepatocytes. These results indicate that CRH is a novel stimulator of somatolactins in teleost pituitary cells, and somatolactins may participate in the stress response by regulating energy metabolism.
Imidacloprid Induces Lysosomal Dysfunction and Cell Death in Human Astrocytes and Fibroblasts—Environmental Implication of a Clinical Case Report
Authors: Eriksson Ida , Ward Liam J. , Vainikka Linda , Sultana Nargis , Leanderson Per , Flodin Ulf , Li Wei , Yuan Xi-Ming
Published: December 2023

Imidacloprid (IMI), a neonicotinoid insecticide, has potential cytotoxic and genotoxic effects on human and experimental models, respectively. While being an emerging environmental contaminant, occupational exposure and related cellular mechanisms are unknown. Herein, we were motivated by a specific patient case where occupational exposure to an IMI-containing plant protection product was associated with the diagnosis of Bell’s palsy. The aim was to investigate the toxic effects and cellular mechanisms of IMI exposure on glial cells (D384 human astrocytes) and on human fibroblasts (AG01518). IMI-treated astrocytes showed a reduction in cell number and dose-dependent cytotoxicity at 24 h. Lower doses of IMI induced reactive oxygen species (ROS) and lysosomal membrane permeabilisation (LMP), causing apoptosis and autophagic dysfunction, while high doses caused significant necrotic cell death. Using normal fibroblasts, we found that IMI-induced autophagic dysfunction and lysosomal damage, activated lysophagy, and resulted in a compensatory increase in lysosomes. In conclusion, the observed IMI-induced effects on human glial cells and fibroblasts provide a possible link between IMI cytotoxicity and neurological complications observed clinically in the patient exposed to this neonicotinoid insecticide.
Open-Label Sulforaphane Trial in FMR1 Premutation Carriers with Fragile-X-Associated Tremor and Ataxia Syndrome (FXTAS)
Authors: Santos Ellery , Clark Courtney , Biag Hazel Maridith B. , Tang Si Jie , Kim Kyoungmi , Ponzini Matthew D. , Schneider Andrea , Giulivi Cecilia , Montanaro Federica Alice Maria , Gipe Jesse Tran-Emilia , Dayton Jacquelyn , Randol Jamie L. , Yao Pamela J. , Manolopoulos Apostolos , Kapogiannis Dimitrios , Hwang Ye Hyun , Hagerman Paul , Hagerman Randi , Tassone Flora
Published: December 2023

Fragile X (FMR1) premutation is a common mutation that affects about 1 in 200 females and 1 in 450 males and can lead to the development of fragile-X-associated tremor/ataxia syndrome (FXTAS). Although there is no targeted, proven treatment for FXTAS, research suggests that sulforaphane, an antioxidant present in cruciferous vegetables, can enhance mitochondrial function and maintain redox balance in the dermal fibroblasts of individuals with FXTAS, potentially leading to improved cognitive function. In a 24-week open-label trial involving 15 adults aged 60–88 with FXTAS, 11 participants successfully completed the study, demonstrating the safety and tolerability of sulforaphane. Clinical outcomes and biomarkers were measured to elucidate the effects of sulforaphane. While there were nominal improvements in multiple clinical measures, they were not significantly different after correction for multiple comparisons. PBMC energetic measures showed that the level of citrate synthase was higher after sulforaphane treatment, resulting in lower ATP production. The ratio of complex I to complex II showed positive correlations with the MoCA and BDS scores. Several mitochondrial biomarkers showed increased activity and quantity and were correlated with clinical improvements.
Exploring Strategies for Copper Removal from Nickel Anolytes: A Review
Authors: Tang Xiaowei , Ju Kunyu
Published: December 2023

Various methods, such as electrochemical purification, chemical precipitation, solvent extraction, and ion-exchange resins, have been extensively employed for the removal of copper from nickel anolytes. However, these methods exhibit several significant drawbacks when applied in industrial settings. For instance, electrochemical purification fails to efficiently manage nickel anolyte solutions with low copper content. Chemical precipitation presents challenges in residue management and incurs high production costs for precipitants. Solvent extraction raises concerns related to toxicity, while the use of ion-exchange resins demands meticulous selection of suitable materials. In this review, we present a comprehensive review of the nickel removal methods used for nickel anolyte purification, electrochemical purification, chemical precipitation, solvent extraction, and ion-exchange resins. We also examine the suitability and benefits of each technique in industrial settings. The ion-exchange method has drawn significant attention due to its strong selectivity and small adsorption quantity. The ion-exchange separation process does not generate any slag, and the ion-exchange resin can be recycled and reused; this method has great potential in a wide range of applications.
Laser-Scribed Pencil Lead Electrodes for Amperometric Quantification of Indapamide
Authors: Oliveira Thawan G. , Lima Irlan S. , Ameku Wilson A. , Gonçalves Josué M. , Souza Rodrigo S. , Toma Henrique E. , Angnes Lúcio
Published: December 2023

Laser engraving is a convenient, fast, one-step, and environmentally friendly technique used to produce more conductive surfaces by local pyrolysis. The laser’s thermal treatment can also remove non-conductive materials from the electrode surfaces and improve electrochemical performance. The improvement was assessed by electrochemical tools such as cyclic voltammograms and electrochemical impedance spectroscopy using [Fe(CN)6]3−/4− and dopamine as redox probes. The electrochemical results observed showed that a treated surface showed an improvement in electron transfer and less resistance to charge transfer. To optimize the electrode performance, it was necessary to search for the most favorable graphite mines and optimize the parameters of the laser machine (laser power, scan rate, and output distance). The resultant material was adequately characterized by Raman spectroscopy and scanning electron microscopy (SEM), where an irregular surface composed of crystalline graphite particles was noticed. Furthermore, as a proof-of-concept, it was applied to detect indapamide (IND) in synthetic urine by flow injection analysis (FIA), a diuretic drug often used by athletes to alter urine composition to hide forbidden substance consumption in doping tests.
Metabolic Interplay in the Tumor Microenvironment: Implications for Immune Function and Anticancer Response
Authors: Youssef Reem , Maniar Rohan , Khan Jaffar , Mesa Hector
Published: December 2023

Malignant tumors exhibit rapid growth and high metabolic rates, similar to embryonic stem cells, and depend on aerobic glycolysis, known as the “Warburg effect”. This understanding has enabled the use of radiolabeled glucose analogs in tumor staging and therapeutic response assessment via PET scans. Traditional treatments like chemotherapy and radiotherapy target rapidly dividing cells, causing significant toxicity. Despite immunotherapy’s impact on solid tumor treatment, gaps remain, leading to research on cancer cell evasion of immune response and immune tolerance induction via interactions with the tumor microenvironment (TME). The TME, consisting of immune cells, fibroblasts, vessels, and the extracellular matrix, regulates tumor progression and therapy responses. TME-targeted therapies aim to transform this environment from supporting tumor growth to impeding it and fostering an effective immune response. This review examines the metabolic disparities between immune cells and cancer cells, their impact on immune function and therapeutic targeting, the TME components, and the complex interplay between cancer cells and nontumoral cells. The success of TME-targeted therapies highlights their potential to achieve better cancer control or even a cure.
Impact of Hypoxia-Induced miR-210 on Pancreatic Cancer
Authors: Lian Mutian , Mortoglou Maria , Uysal-Onganer Pinar
Published: December 2023

Pancreatic cancer (PC) poses significant clinical challenges, with late-stage diagnosis and limited therapeutic options contributing to its dismal prognosis. A hallmark feature of PC is the presence of a profoundly hypoxic tumour microenvironment, resulting from various factors such as fibrotic stroma, rapid tumour cell proliferation, and poor vascularization. Hypoxia plays a crucial role in promoting aggressive cancer behaviour, therapeutic resistance, and immunosuppression. Previous studies have explored the molecular mechanisms behind hypoxia-induced changes in PC, focusing on the role of hypoxia-inducible factors (HIFs). Among the myriad of molecules affected by hypoxia, microRNA-210 (miR-210) emerges as a central player. It is highly responsive to hypoxia and regulated by HIF-dependent and HIF-independent pathways. miR-210 influences critical cellular processes, including angiogenesis, metastasis, and apoptosis, all of which contribute to PC progression and resistance to treatment. Understanding these pathways provides insights into potential therapeutic targets. Furthermore, investigating the role of miR-210 and its regulation in hypoxia sheds light on the potential development of early diagnostic strategies, which are urgently needed to improve outcomes for PC patients. This review delves into the complexities of PC and introduces the roles of hypoxia and miR-210 in the progression of PC.
The Translation, Validation and Cultural Adaptation of Questionnaires Assessing the Quality of Life and Fatigue among Patients with Sjogren’s Syndrome for the Romanian Context
Authors: Mardale Denise-Ani , Opriș-Belinski Daniela , Bojincă Violeta , Bojincă Mihai , Păsăran Emilia , Săulescu Ioana , Berghea Florian , Bălănescu Andra
Published: December 2023

About 70% of patients with Sjogren’s syndrome suffer from fatigue, and for a large proportion of patients, it is one of the most noticeable manifestations leading to disability. To date, no study has been conducted in Romania to determine the quality of life of patients and the impact of fatigue in patients with Sjogren’s syndrome. The present work proposes the translation, cultural adaptation, and validation of two questionnaires for the Romanian context, namely the ‘Profile of Fatigue and Discomfort–Sicca Symptoms Inventory’ (PROFAD-SSI-SF) and ‘Primary Sjogren’s Syndrome—Quality of Life’ (PSS-QoL), whose purpose is to assess quality of life and fatigue in patients with Sjogren’s syndrome. These two questionnaires were administered to 52 patients with Sjogren’s syndrome diagnosed according to the 2016 ACR-EULAR criteria. Subsequently, the conceptual, semantic, and operational analyses of the data were performed with the aim of cultural adaptation. The data obtained were statistically analyzed using indices of measurement accuracy such as internal consistency. Based on statistical analyses, this pilot study shows that the Romanian versions of the PROFAD-SSI and PSS-QoL questionnaires are as reliable as their English counterparts.
Protecting Street Art from Outdoor Environmental Threats: What Are the Challenges?
Authors: Pagnin Laura , Guarnieri Nicolò , Izzo Francesca Caterina , Goidanich Sara , Toniolo Lucia
Published: December 2023

Street Art is an artistic expression in constant development, whose interest has grown in recent years among society, public administrations, conservators, and heritage scientists. This growing awareness has given rise to a series of debates between professionals with the intent to expand the knowledge relating to conservation practices and possible protection solutions. Additionally, the paint materials used by contemporary artists are in constant development; therefore, difficulty has emerged in the identification of their degradation processes when exposed to environmental conditions and in the consequent selection of a specific protection system. This review presents an overview of the recent literature and experiences in the field of knowledge and preservation of Street Art, focusing on the type and nature of paint formulations, the main deterioration processes of painted artworks in outdoor conditions, and the most recent advances in materials and methods for the conservation and protection of Street Art. This review aims to emphasise how the approach to the challenge of preservation of Street Art is complex, aspiring to the need for optimised diagnostic protocols for the development of innovative and effective protective coatings. This paper is a starting point to provide suggestions and indications for the development of further research projects within the framework of preservation and protection of contemporary muralism.