bluuebunny/crossref_metadata_2025 huggingface dataset:
Elucidating the Influence of Electric Fields toward CO2 Activation on YSZ (111)
Authors: Ulumuddin Nisa , Che Fanglin , Yang Jung-Il , Ha Su , McEwen Jean-Sabin
Published: February 2021

Despite its high thermodynamic stability, the presence of a negative electric field is known to facilitate the activation of CO2 through electrostatic effects. To utilize electric fields for a reverse water gas shift reaction, it is critical to elucidate the role of an electric field on a catalyst surface toward activating a CO2 molecule. We conduct a first-principles study to gain an atomic and electronic description of adsorbed CO2 on YSZ (111) surfaces when external electric fields of +1 V/Å, 0 V/Å, and −1 V/Å are applied. We find that the application of an external electric field generally destabilizes oxide bonds, where the direction of the field affects the location of the most favorable oxygen vacancy. The direction of the field also drastically impacts how CO2 adsorbs on the surface. CO2 is bound by physisorption when a +1 V/Å field is applied, a similar interaction as to how it is adsorbed in the absence of a field. This interaction changes to chemisorption when the surface is exposed to a −1 V/Å field value, resulting in the formation of a CO3− complex. The strong interaction is reflected through a direct charge transfer and an orbital splitting within the Olatticep-states. While CO2 remains physisorbed when a +1 V/Å field value is applied, our total density of states analysis indicates that a positive field pulls the charge away from the adsorbate, resulting in a shift of its bonding and antibonding peaks to higher energies, allowing a stronger interaction with YSZ (111). Ultimately, the effect of an electric field toward CO2 adsorption is not negligible, and there is potential in utilizing electric fields to favor the thermodynamics of CO2 reduction on heterogeneous catalysts.
Progress in Catalytic Hydrodechlorination
Authors: Martin-Martinez Maria , Gómez-Sainero Luisa M.
Published: February 2021

Catalytic hydrodechlorination (HDC) is a technology with great potential for the treatment and valorization of organic chlorinated wastes [...]
Synthesis of Ethylene/1-Octene Copolymers with Ultrahigh Molecular Weights by Zr and Hf Complexes Bearing Bidentate NN Ligands with the Camphyl Linker
Authors: Feng Chunyu , Gou Qingqiang , Liu Shaofeng , Gao Rong , Li Zhibo
Published: February 2021

Ultrahigh molecular weight polyethylene (UHMWPE) is a class of high-performance engineering plastics, exhibiting a unique set of properties and applications. Although many advances have been achieved in recent years, the synthesis of UHMWPE is still a great challenge. In this contribution, a series of zirconium and hafnium complexes, [2,6-(R1)2-4-R2-C6H2-N-C(camphyl)=C(camphyl)-N-2,6-(R1)2-4-R2-C6H2]MMe2(THF) (1-Zr: R1 = Me, R2 = H, M = Zr; 2-Zr: R1 = Me, R2 = Me, M = Zr; 1-Hf: R1 = Me, R2 = H, M = Hf; 2-Hf: R1 = Me, R2 = Me, M = Hf), bearing bidentate NN ligands with the bulky camphyl backbone were synthesized by the stoichiometric reactions of α-diimine ligands with MMe4 (M = Hf or Zr). All Zr and Hf metal complexes were analyzed using 1H and 13C NMR spectroscopy, and the molecular structures of complexes 1-Zr and 1-Hf were determined by single-crystal X-ray diffraction, revealing that the original α-diimine ligand was selectively reduced into the ene-diamido form and generated an 1,3-diaza-2-metallocyclopentene ring in the metal complexes. Zr complexes 1-Zr and 2-Zr showed moderate activity (up to 388 kg(PE)·mol−1(M)·h−1), poor copolymerization ability, but unprecedented molecular weight capability toward ethylene/1-octene copolymerization. Therefore, copolymers with ultrahigh molecular weights (>600 or 337 × 104 g∙mol−1) were successfully synthesized by 1-Zr or 2-Zr, respectively, with the borate cocatalyst [Ph3C][B(C6F5)4]. Surprisingly, Hf complexes 1-Hf and 2-Hf showed negligible activity under otherwise identical conditions, revealing the great influence of metal centers on catalytic performances.
Status Update on Bioelectrochemical Systems: Prospects for Carbon Electrode Design and Scale-Up
Authors: Herkendell Katharina
Published: February 2021

Bioelectrochemical systems (BES) employ enzymes, subcellular structures or whole electroactive microorganisms as biocatalysts for energy conversion purposes, such as the electrosynthesis of value-added chemicals and power generation in biofuel cells. From a bioelectrode engineering viewpoint, customizable nanostructured carbonaceous matrices have recently received considerable scientific attention as promising electrode supports due to their unique properties attractive to bioelectronics devices. This review demonstrates the latest advances in the application of nano- and micro-structured carbon electrode assemblies in BES. Specifically, in view of the gradual increase in the commercial applicability of these systems, we aim to address the stability and scalability of different BES designs and to highlight their potential roles in a circular bioeconomy.
Influence of Recombinant Codon-Optimized Plasmid DNA Encoding VEGF and FGF2 on Co-Induction of Angiogenesis
Authors: Salafutdinov Ilnur I. , Gazizov Ilnaz M. , Gatina Dilara K. , Mullin Ruslan I. , Bogov Alexey A. , Islamov Rustem R. , Kiassov Andrey P. , Masgutov Ruslan F. , Rizvanov Albert A.
Published: February 2021

Several methods for the stimulation of skin wound repair have been proposed over the last few decades. The most promising among them are gene and stem cell therapy. Our present experiments combined several approaches via the application of human umbilical cord blood mononuclear cells (hUCB-MC) that were transfected with pBud-VEGF165-FGF2 plasmid (gene-cell therapy) and direct gene therapy using pBud-VEGF165-FGF2 plasmid to enhance healing of full thickness skin wounds in rats. The dual expression cassette plasmid pBud-VEGF165-FGF2 encodes both VEGF and FGF2 therapeutic genes, expressing pro-angiogenic growth factors. Our results showed that, with two weeks post-transplantation, some transplanted cells still retained expression of the stem cell and hematopoietic markers C-kit and CD34. Other transplanted cells were found among keratinocytes, hair follicle cells, endothelial cells, and in the derma. PCNA expression studies revealed that transplantation of transfected cells terminated proliferative processes in regenerating wounds earlier than transplantation of untransfected cells. In the direct gene therapy group, four days post-operatively, the processes of flap revascularization, while using Easy LDI Microcirculation Camera, was higher than in control wounded skin. We concluded that hUCB-MC can be used for the treatment of skin wounds and transfection these cells with VEGF and FGF2 genes enhances their regenerative abilities. We also concluded that the application of pBud-VEGF165-FGF2 plasmids is efficient for the direct gene therapy of skin wounds by stimulation of wound revascularization.
Macrophages in Lung Injury, Repair, and Fibrosis
Authors: Cheng Peiyong , Li Shuangyan , Chen Huaiyong
Published: February 2021

Fibrosis progression in the lung commonly results in impaired functional gas exchange, respiratory failure, or even death. In addition to the aberrant activation and differentiation of lung fibroblasts, persistent alveolar injury and incomplete repair are the driving factors of lung fibrotic response. Macrophages are activated and polarized in response to lipopolysaccharide- or bleomycin-induced lung injury. The classically activated macrophage (M1) and alternatively activated macrophage (M2) have been extensively investigated in lung injury, repair, and fibrosis. In the present review, we summarized the current data on monocyte-derived macrophages that are recruited to the lung, as well as alveolar resident macrophages and their polarization, pyroptosis, and phagocytosis in acute lung injury (ALI). Additionally, we described how macrophages interact with lung epithelial cells during lung repair. Finally, we emphasized the role of macrophage polarization in the pulmonary fibrotic response, and elucidated the potential benefits of targeting macrophage in alleviating pulmonary fibrosis.
Inflammation-Related Risk Loci in Genome-Wide Association Studies of Coronary Artery Disease
Authors: Mauersberger Carina , Schunkert Heribert , Sager Hendrik B.
Published: February 2021

Although the importance of inflammation in atherosclerosis is now well established, the exact molecular processes linking inflammation to the development and course of the disease are not sufficiently understood. In this context, modern genetics—as applied by genome-wide association studies (GWAS)—can serve as a comprehensive and unbiased tool for the screening of potentially involved pathways. Indeed, a considerable proportion of loci discovered by GWAS is assumed to affect inflammatory processes. Despite many well-replicated association findings, however, translating genomic hits to specific molecular mechanisms remains challenging. This review provides an overview of the currently most relevant inflammation-related GWAS findings in coronary artery disease and explores their potential clinical perspectives.
Tribocorrosion Performance of Cr/CrN Hybrid Layer as a Coating for Machine Components Used in a Chloride Ions Environment
Authors: Kowalski Marcin , Stachowiak Arkadiusz
Published: February 2021

The aim of the article was to identify the effect of material hardness on the tribocorrosion process by comparing two material solutions. The analysis concerned the assessment of the process intensity and the identification of the mechanisms responsible for material loss. Possible mechanisms of tribocorrosion common for materials of high hardness were determined. Two classic material solutions (based on AISI 1045 steel) ensuring high hardness of the subsurface layers were tested: nitriding with an additional oxidation and impregnation process, and Physical Vapour Deposition (PVD) coating. In order to better identify the impact of hardness on the tribocorrosion process in each individual test, the pressures in the contact zone were increased. The tribocorrosion tests were carried out in 3.5% NaCl with free corrosion potential (OCP) for the ball-on-plate system. The results of the tribocorrosion tests presented in the article indicate that the synergy effect of friction and corrosion can be generated by the same mechanisms of material removal in both the material solutions tested. The intensity of these mechanisms is determined by material hardness. The likely mechanism of generating the synergy effect may be related to the formation of local pits along the friction path. The corrosion processes that are initiated by the cracking of the hard surface layer create local cavities, which most probably intensify frictional wear in successive time intervals. The area around the cavities facilitates plastic deformation, the initiation of cracking of the cyclically deformed layer and the tearing of larger pieces of material (especially at higher unit pressures in the frictional contact zone).
Performance and Tolerability of a New Topical Dexpanthenol-Containing Emollient Line in Subjects with Dry Skin: Results from Three Randomized Studies
Authors: Stettler Hans , de Salvo Raffaella , Olsavszky Rozalia , Nanu Elena Alina , Dumitru Veronica , Trapp Sonja
Published: February 2021

Three studies were conducted with three new dexpanthenol-containing emollients containing increasing lipid contents (Emollients 1–3) to assess their performances in healthy adults with dry skin. All three studies (N = 42 each) followed virtually the same design. A single skin application of the study product was performed followed by once-daily usage. Skin hydration, transepidermal water loss (TEWL), skin biomechanical properties, and lipid content of the stratum corneum (SC) were regularly assessed over the 28-day study period; a subset (N = 22) underwent a sodium lauryl sulfate (SLS) challenge prior to product application. All three emollients were well tolerated and showed good performances with only minor differences in instrumental measurements. After single and prolonged once-daily applications of Emollients 1–3 to dry skin and dry SLS-damaged skin, skin hydration significantly increased from baseline (BL) (by 38.1–72.4% in unchallenged skin, p < 0.001 for all three). This was paralleled by significant increases in skin elasticity parameters. Usage of Emollients 1 and 3 caused increases from BL in SC cholesterol (by 9.8–12.5%, p < 0.05 for both) and SC free fatty acid levels (by 3.7–26.3%, p < 0.05 for both) at the end of the study. No sustained effects on TEWL were recorded. Our findings support the once-daily use of all three emollients in adults with dry skin.
Encapsulation of Calcium Phosphates on Electrospun Nanofibers for Tissue Engineering Applications
Authors: Nathanael Arputharaj Joseph , Oh Tae Hwan
Published: February 2021

In the field of tissue engineering, electrospinning is a versatile technique that provides nanofibers with structure similar to that of the extracellular matrix owing to their flexible functionalization. Considerable developments in electrospinning have been made to produce engineered electrospun nanofibers for different biomedical applications. Various biopolymers possess good biocompatibility and biodegradability and are nontoxic in nature. Modification of these biopolymers can enhance or elicit certain properties. One technique of modification is the incorporation of certain inorganic ions or components that can enhance its specific functional characteristics such as mineralization, osseointegration, and bioactivity. Incidentally, calcium phosphate (CaP) materials have proven to be suitable and versatile for biopolymer incorporation and exploration because of their inherent bioactivity and being key mineral constituents of bone and teeth. The addition of CaP materials to polymers enhances cell infiltration, differentiation, and biomineralization. We aim to provide a broad overview of CaP material (particularly hydroxyapatite (HA))-incorporated electrospun nanocomposite fibers and their possible applications in tissue engineering. Some key polymer/HA composites were discussed in detail, and a brief discussion on other polymer/HA composites was also provided. Finally, we discussed the future perspectives of this interesting and emerging composite material fabricated via electrospinning.
Effect of the Dimensions of Coplanar Inner Floating Ring Electrode on the Performance of Liquid Crystal Lenses
Authors: Hsu Yung-Hsiang , Chen Bo-Yu , Sheu Chia-Rong
Published: February 2021

In this study, we mainly investigated the effect of the dimensions of a coplanar inner floating ring (CIFR) on the lens performance in CIFR–hole-patterned electrode liquid crystal lenses (HPELCLs) at 100 Hz of the electrical driving frequency. The operation and threshold voltages in CIFR–HPELCLs are approximately 76% compared with those of the conventional HPELCL. The diameter of the CIFR with 360 μm in relation to imaging capabilities and those of the conventional glass lens and HPELCL were analyzed via the modulation transfer function. The relative mechanisms of the CIFR dimensions and the lens performance were also examined. An electric circuit model was used to analyze and illustrate the experimental results.
Phase Behaviour of Methane Hydrates in Confined Media
Authors: Bian Hao , Ai Lu , Hellgardt Klaus , Maitland Geoffrey C. , Heng Jerry Y. Y.
Published: February 2021

In a study designed to investigate the melting behaviour of natural gas hydrates which are usually formed in porous mineral sediments rather than in bulk, hydrate phase equilibria for binary methane and water mixtures were studied using high-pressure differential scanning calorimetry in mesoporous and macroporous silica particles having controlled pore sizes ranging from 8.5 nm to 195.7 nm. A dynamic oscillating temperature method was used to form methane hydrates reproducibly and then determine their decomposition behaviour—melting points and enthalpies of melting. Significant decreases in dissociation temperature were observed as the pore size decreased (over 6 K for 8.5 nm pores). This behaviour is consistent with the Gibbs–Thomson equation, which was used to determine hydrate–water interfacial energies. The melting data up to 50 MPa indicated a strong, essentially logarithmic, dependence on pressure, which here has been ascribed to the pressure dependence of the interfacial energy in the confined media. An empirical modification of the Gibbs–Thomson equation is proposed to include this effect.
Influence of HAP on the Morpho-Structural Properties and Corrosion Resistance of ZrO2-Based Composites for Biomedical Applications
Authors: Barabás Réka , Fort Carmen Ioana , Turdean Graziella Liana , Bizo Liliana
Published: February 2021

In the present work, ZrO2-based composites were prepared by adding different amounts of antibacterial magnesium oxide and bioactive and biocompatible hydroxyapatite (HAP) to the inert zirconia. The composites were synthesized by the conventional ceramic processing route and morpho-structurally analyzed by X-ray powder diffraction (XRPD) and scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). Two metallic dental alloys (i.e., Ni–Cr and Co–Cr) coated with a chitosan (Chit) membrane containing the prepared composites were exposed to aerated artificial saliva solutions of different pHs (i.e., 4.3, 5, 6) and the corrosion resistances were investigated by electrochemical impedance spectroscopy technique. The obtained results using the two investigated metallic dental alloys shown quasi-similar anticorrosive properties, having quasi-similar charge transfer resistance, when coated with different ZrO2-based composites. This behavior could be explained by the synergetic effect between the diffusion process through the Chit-composite layer and the roughness of the metallic electrode surface.
Optimized Identification of High-Grade Prostate Cancer by Combining Different PSA Molecular Forms and PSA Density in a Deep Learning Model
Authors: Gentile Francesco , Ferro Matteo , Della Ventura Bartolomeo , La Civita Evelina , Liotti Antonietta , Cennamo Michele , Bruzzese Dario , Velotta Raffaele , Terracciano Daniela
Published: February 2021

After skin cancer, prostate cancer (PC) is the most common cancer among men. The gold standard for PC diagnosis is based on the PSA (prostate-specific antigen) test. Based on this preliminary screening, the physician decides whether to proceed with further tests, typically prostate biopsy, to confirm cancer and evaluate its aggressiveness. Nevertheless, the specificity of the PSA test is suboptimal and, as a result, about 75% of men who undergo a prostate biopsy do not have cancer even if they have elevated PSA levels. Overdiagnosis leads to unnecessary overtreatment of prostate cancer with undesirable side effects, such as incontinence, erectile dysfunction, infections, and pain. Here, we used artificial neuronal networks to develop models that can diagnose PC efficiently. The model receives as an input a panel of 4 clinical variables (total PSA, free PSA, p2PSA, and PSA density) plus age. The output of the model is an estimate of the Gleason score of the patient. After training on a dataset of 190 samples and optimization of the variables, the model achieved values of sensitivity as high as 86% and 89% specificity. The efficiency of the method can be improved even further by training the model on larger datasets.
Comprehensive Analysis of the Expression of Key Genes Related to Hippo Signaling and Their Prognosis Impact in Ovarian Cancer
Authors: Kubelac Paul , Braicu Cornelia , Raduly Lajos , Chiroi Paul , Nutu Andreea , Cojocneanu Roxana , Budisan Liviuta , Berindan-Neagoe Ioana , Achimas-Cadariu Patriciu
Published: February 2021

The Hippo signaling pathway, one of the most conserved in humans, controlling dimensions of organs and tumor growth, is frequently deregulated in several human malignancies, including ovarian cancer (OC). The alteration of Hippo signaling has been reported to contribute to ovarian carcinogenesis and progression. However, the prognostic roles of individual Hippo genes in OC patients remain elusive. Herein we investigated the expression level and prognostic value of key Hippo genes in OC using online databases, followed by a qRT-PCR validation step in an additional patient cohort. Using the GEPIA database, we observed an increased level for TP53 and reduced expression level for LATS1, LATS2, MST1, TAZ, and TEF in tumor tissue versus normal adjacent tissue. Moreover, LATS1, LATS2, TP53, TAZ, and TEF expression levels have prognostic significance correlated with progression-free survival. The qRT-PCR validation step was conducted in an OC patient cohort comprising 29 tumor tissues and 20 normal adjacent tissues, endorsing the expression level for LATS1, LATS2, and TP53, as well as for two of the miRNAs targeting the TP53 gene, revealing miR-25-3p upregulation and miR-181c-5p downregulation. These results display that there are critical prognostic value dysregulations of the Hippo genes in OC. Our data demonstrate the major role the conserved Hippo pathway presents in tumor control, underlying potential therapeutic strategies and controlling several steps modulated by miRNAs and their target genes that could limit ovarian cancer progression.
Diagnostic Strategies for Treatment Selection in Advanced Prostate Cancer
Authors: McNevin Ciara S. , Baird Anne-Marie , McDermott Ray , Finn Stephen P.
Published: February 2021

Prostate Cancer (PCa) is a leading cause of morbidity and mortality among men worldwide. For most men with PCa, their disease will follow an indolent course. However, advanced PCa is associated with poor outcomes. There has been an advent of new therapeutic options with proven efficacy for advanced PCa in the last decade which has improved survival outcomes for men with this disease. Despite this, advanced PCa continues to be associated with a high rate of death. There is a lack of strong evidence guiding the timing and sequence of these novel treatment strategies. This paper focuses on a review of the strategies for diagnostic and the current evidence available for treatment selection in advanced PCa.
An Efficient and Accurate Depth-Wise Separable Convolutional Neural Network for Cybersecurity Vulnerability Assessment Based on CAPTCHA Breaking
Authors: Dankwa Stephen , Yang Lu
Published: February 2021

Cybersecurity practitioners generate a Completely Automated Public Turing test to tell Computers and Humans Apart (CAPTCHAs) as a form of security mechanism in website applications, in order to differentiate between human end-users and machine bots. They tend to use standard security to implement CAPTCHAs in order to prevent hackers from writing malicious automated programs to make false website registrations and to restrict them from stealing end-users’ private information. Among the categories of CAPTCHAs, the text-based CAPTCHA is the most widely used. However, with the evolution of deep learning, it has been so dramatic that tasks previously thought not easily addressable by computers and used as CAPTCHA to prevent spam are now possible to break. The workflow of CAPTCHA breaking is a combination of efforts, approaches, and the development of the computation-efficient Convolutional Neural Network (CNN) model that attempts to increase accuracy. In this study, in contrast to breaking the whole CAPTCHA images simultaneously, this study split four-character CAPTCHA images for the individual characters with a 2-pixel margin around the edges of a new training dataset, and then proposed an efficient and accurate Depth-wise Separable Convolutional Neural Network for breaking text-based CAPTCHAs. Most importantly, to the best of our knowledge, this is the first CAPTCHA breaking study to use the Depth-wise Separable Convolution layer to build an efficient CNN model to break text-based CAPTCHAs. We have evaluated and compared the performance of our proposed model to that of fine-tuning other popular CNN image recognition architectures on the generated CAPTCHA image dataset. In real-time, our proposed model used less time to break the text-based CAPTCHAs with an accuracy of more than 99% on the testing dataset. We observed that our proposed CNN model has efficiently improved the CAPTCHA breaking accuracy and streamlined the structure of the CAPTCHA breaking network as compared to other CAPTCHA breaking techniques.
Research on the Cascade Vehicle Detection Method Based on CNN
Authors: Hu Jianjun , Sun Yuqi , Xiong Songsong
Published: February 2021

This paper introduces an adaptive method for detecting front vehicles under complex weather conditions. In the field of vehicle detection from images extracted by cameras installed in vehicles, backgrounds with complicated weather, such as rainy and snowy days, increase the difficulty of target detection. In order to improve the accuracy and robustness of vehicle detection in front of driverless cars, a cascade vehicle detection method combining multifeature fusion and convolutional neural network (CNN) is proposed in this paper. Firstly, local binary patterns, Haar-like and orientation gradient histogram features from the front vehicle are extracted, then principal-component-analysis dimension reduction and serial-fusion processing are performed on the input image. Furthermore, a preliminary screening is conducted as the input of a support vector machine classifier based on the acquired fusion features, and the CNN model is employed to validate cascade detection of the filtered results. Finally, an integrated data set extracted from BDD, Udacity, and other data sets is utilized to test the method proposed. The recall rate is 98.69%, which is better than the traditional feature algorithm, and the recall rate of 97.32% in a complex driving environment indicates that the algorithm possesses good robustness.
Novel Switching Frequency FCS-MPC of PMSG for Grid-Connected Wind Energy Conversion System with Coordinated Low Voltage Ride Through
Authors: Ghany Asmaa A. , Shehata E. G. , Elsayed Abo-Hashima M. , Mohamed Yahia S. , Haes Alhelou Hassan , Siano Pierluigi , Diab Ahmed A. Zaki
Published: February 2021

The integration of wind energy systems (WECS) into the power grid through power electronic converters should ensure the high performance of the control system. In spite of several advantages of conventional Finite control set-model predictive controller (FCS-MPC), variable switching frequency and high computational burden are considered its main drawbacks. In this paper, a fast FCS-MPC of a machine side converter (MSC) of direct-driven permanent magnet synchronous generator (PMSG) based wind turbines for wind energy conversion system is proposed. The wind energy conversion system has been realized using a direct driven PMSG and a full-scale back-to-back power converter. The proposed controller is designed to reduce the required calculations in each horizon. In addition, the performance of conventional FCS-MPC is compared with the proposed method, and an improvement in total harmonic distortion spectra and simulation time required even when imposing a lower sampling frequency was found. To overcome the variable switching frequency problem, a modulation algorithm is introduced in the minimization process of modulated FCS-MPC. To keep the proposed system attached to the utility during a fault, a coordinated pitch angle control and low voltage-ride through (LVRT) algorithm is designed and inserted in the vector control of the grid side converter (GSC) to supply reactive power to the grid during fault for ensuring safe operation of the inverter and meeting the grid code requirements. The effectiveness of the proposed controller is illustrated using simulation results under different operating conditions.
The Impact of Terroir on the Flavour of Single Malt Whisk(e)y New Make Spirit
Authors: Kyraleou Maria , Herb Dustin , O’Reilly Grace , Conway Neil , Bryan Tom , Kilcawley Kieran N.
Published: February 2021

The impact of barley variety and its geographical growth location (environment) on the flavour of new make spirit was investigated to determine if “terroir” can be applied in the production of single malt whisk(e)y. New make spirits were produced at laboratory scale under controlled conditions from two different barley varieties (Olympus and Laureate) grown at two distinct environments (Athy, Co Kildare and Bunclody, Co Wexford) in Ireland over two consecutive seasons (2017 and 2018). The spirit samples were analysed by gas chromatography mass spectrometry olfactometry and descriptive sensory analysis. Forty-two volatiles were detected with eight deemed as very influential and fifteen deemed as influential to the aroma of new make spirit. Sensory attributes were influenced by barley variety, environment, and the interactions thereof over both seasons, with environment and the interaction of variety x environment having a greater impact than variety alone. Chemometric analysis of the olfactometry and sensory data found that both environment and season had a greater impact on the aromatic sensory perception of the new make spirits than variety alone. Therefore, this study clearly demonstrates a “terroir” impact on the flavour of new make spirit and highlights its potential importance especially in relation to single malt whisk(e)y.
Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia
Authors: Saddoud Debbabi Olfa , Rahmani Mnasri Sameh , Ben Amar Fathi , Ben Naceur M’barek , Montemurro Cinzia , Miazzi Monica Marilena
Published: February 2021

Among the countries of the Mediterranean Basin, Tunisia is located at the crossroad for the immigration of several civilizations over the last two millennia, becoming a strategic place for gene flow, and a secondary center of diversity for olive species. Olive is one of the principal crop species in Tunisia and now it strongly characterizes the rural landscape of the country. In recent years, collecting missions on farm and in situ were carried out by various institutes, with special emphasis given to ex situ collections serving as a reference for the identification of olive germplasm. Simple Sequence Repeats (SSRs) represent the easiest and cheapest markers for olive genetic fingerprinting and have been the tool of choice for studying the genetic diversity of this crop in Tunisia, to resolve cases of homonymy and synonymy among the commercialized varieties, to identify rare cultivars, to improve knowledge about the genetic variability of this crop, to identify a hot spot of olive biodiversity in the Tunisian oasis of Degache, and to enrich the national reference collection of olive varieties. The present review describes the state of the art of the genetic characterization of the Tunisian olive germplasm and illustrate the progress obtained through the SSR markers, in individuating interesting genotypes that could be used for facing incoming problems determined by climate changes.
Validation of an Index for Susceptibility to Earthquake-Induced Landslides Derived from Helicopter-Borne Electromagnetic Resistivity and Digital Elevation Data
Authors: Nonomura Atsuko , Hasegawa Shuichi , Abe Tatsuya , Mukoyama Sakae , Kaneda Yoshiyuki
Published: February 2021

Catastrophic earthquake-induced landslides can occur on slopes composed of loosened and fractured rock masses. Although it is impossible to prevent such landslides, estimation of the susceptibility of slopes to earthquake-induced landslides is useful for risk management. An index of susceptibility to earthquake-induced landslides (ISEL) was developed by using helicopter-borne electromagnetic resistivity data. However, the ISEL has not yet been validated through the analysis of pre-earthquake data. In this study, ISEL values were estimated from resistivity and digital elevation data obtained in 2013 around Mt. Aso, Kyushu, before the 2016 Kumamoto earthquake. Although most of the landslides around Mt. Aso during the 2016 Kumamoto earthquake were mass movements of volcanic tephra layers, some of them occurred on loosened rock masses. Landslide susceptible areas at loosened rock masses are the target for ISEL value estimation. Our results validated the effectiveness of the ISEL as a predictor of earthquake-induced rock mass landslides.
Reframing Entrepreneurship via Identity, Techné, and Material Culture
Authors: Ruiz Victoria E.
Published: February 2021

Entrepreneurship is typically understood as capitalist, but new models are emerging; these new models, like Welter et al.’s “everyday-entrepreneur,” can be understood in the tradition of techné, in which entrepreneurship is an embodied practice balancing the sociality of identity politics and the materiality of objects and infrastructures. With no English equivalent, techné is typically understood as either art, skill or craft, but none of the placeholders provide a suitable encapsulation of the term itself (Pender). Examining identity against the backdrop of entrepreneurship illuminates the rhetorical ways entrepreneurs cultivate and innovate the processes of making, especially in terms of the material cultures that this process springs from and operates within. Intersectional issues related to entrepreneurial identity present opportunities for diversification and growth in the existing scholarship. A reframing of entrepreneurial identity and continued development of Welter et al.’s everyday-entrepreneurship is argued for, showing how social biases render gender and objects invisible. The article uses data from an on-going study to demonstrate how reframing entrepreneurial identity uncovers the ways in which systemic biases are embedded in the relationship between identity and everyday things. The case study delves into connections between identity, technology, and innovation illustrating how entrepreneurial identity can be seen as a kind of techné, which helps readers better understand identity in relation to material objects and culture—including the biases at work there.
ECG Enhancement and R-Peak Detection Based on Window Variability
Authors: Wu Lu , Xie Xiaoyun , Wang Yinglong
Published: February 2021

In ECG applications, the correct recognition of R-peaks is extremely important for detecting abnormalities, such as arrhythmia and ventricular hypertrophy. In this work, a novel ECG enhancement and R-peak detection method based on window variability is presented, and abbreviated as SQRS. Firstly, the ECG signal corrupted by various high or low-frequency noises is denoised by moving-average filtering. Secondly, the window variance transform technique is used to enhance the QRS complex and suppress the other components in the ECG, such as P/T waves and noise. Finally, the signal, converted by window variance transform, is applied to generate the R-peaks candidates, and the decision rules, including amplitude and kurtosis adaptive thresholds, are applied to determine the R-peaks. A special squared window variance transform (SWVT) is proposed to measure the signal variability in a certain time window, and this technique reduces false detection rate caused by the various types of interference presented in ECG signals. For the MIT-BIH arrhythmia database, the sensitivity of R-peak detection can reach 99.6% using the proposed method.
Quality of Information for Skin Cancer Prevention: A Quantitative Evaluation of Internet Offerings
Authors: Uter Wolfgang , Eversbusch Christina , Gefeller Olaf , Pfahlberg Annette
Published: February 2021

(1) Background: Different sources of information are used by the population regarding skin cancer prevention. The pertinent quality of information that can be retrieved via an internet search engine needs assessment; (2) Methods: Four topical searches in common language were entered into Google™. The first 200 “hits” were stored for further use. Eligible websites were evaluated using content-based criteria based on the current German medical guideline “Skin cancer prevention” and employing generic (DISCERN, HONcode) quality criteria. (3) Results: Overlap between the four search results was between 0 and 7 of 200. The completeness of relevant content was scored with a median of 10 points (first quartile (Q1):6; Q3:14) and thus, it was much lower than the theoretical maximum of 43 points. Global quality, with a maximum of 10 points, was 3 in median (Q1:2; Q3:4). Quality and completeness, respectively, were somewhat higher in the higher ranks of search results. The generic quality was moderate. (4) Conclusions: A direct comparison with other sources of information (print, audio-visual, presentation, or personal counselling) is not possible, but important deficits concerning the quality and scope of relevant information on the internet are demonstrated.
Attunement and Paternal Characteristics in Care Relationships in the Presence of Children Diagnosed with Autism
Authors: Di Renzo Magda , Guerriero Viviana , Pagnacco Andrea , Petrillo Massimiliano , Racinaro Lidia , D’Errico Simona , Bianchi di Castelbianco Federico
Published: February 2021

Parents of children with autism spectrum disorder (ASD) have to address various challenges mainly due to their children’s atypia related to communication, emotion regulation and behaviors, arising also within the relationship with their caregivers. Several studies have pointed out that children with ASD can exhibit many difficulties regarding initiating and maintaining meaningful relationships with others. To date, little research has explored the interactions between children with ASD and their fathers, focusing more on mothers. In the context of parent–child interactions in the presence of autism, recent studies have highlighted the importance of parental attunement but there is little research considering solely the affective-bodily dimension. Therefore, the aim of the present study was to explore the parental attunement in fathers of children with ASD observed during play interactions and to investigate the relationship between paternal attunement and the perception of their psychological characteristics related to care relationships. The results highlight that fathers who describe themselves as better in affective care and sensitivity toward others more likely have an absence of paternal attunement during play interactions. The data presented are discussed in the light of intervention hypotheses, aimed at improving the relationship between fathers and children with autism.
The Impact of Equity Information as An Important Factor in Assessing Business Performance
Authors: Tasáryová Katarína , Pakšiová Renáta
Published: February 2021

Assessing the business performance is an important aspect of almost all economic decisions at the microeconomic and macroeconomic level, in the short and long term. Information about the partners’ relationship to the business, their interest in the evaluation of investments can be explained by various indicators. It is relevant to understand the dependencies of the business performance and the amount of equity, while negative equity can be considered as critical information of existence. The purpose of quantitative research is to identify the relationship between reported negative equity and the business performance in Slovakia on an exhaustive sample of financial data of businesses with negative equity in the period 2014–2018. The business performance with negative equity is assessed through the Altman Z-score and the IN05 index, by classifying businesses into bankruptcy, prosperity and gray zones. Pearson’s correlation analysis between negative equity and Altman Z-score performance confirms the strong direct relationship between negative equity and the bankruptcy zone, the weaker indirect relationship between negative equity and the gray zone, and almost no dependence of negative equity and prosperity zone. In the case of the IN05 index, a low correlation was found between negative equity and all three zones. Although businesses with negative equity are in a bankruptcy zone, they do not have to close automatically, but they have to improve resource management, in particular to increase equity, for example by making a profit and good financial management.
Unraveling the Mode of Action of Cordyceps fumosorosea: Potential Biocontrol Agent against Plutella xylostella (Lepidoptera: Plutellidae)
Authors: Lei Yanyuan , Hussain Abid , Guan Zhaoying , Wang Desen , Jaleel Waqar , Lyu Lihua , He Yurong
Published: February 2021

The entomopathogenic fungus, Cordyceps fumosorosea is a potential eco-friendly biocontrol agent. The present study revealed the entire course of infection of P. xylostella by C. fumosorosea with particular reference to cuticular penetration. Comparative studies on the infection of Plutella xylostella larvae by two strains of C. fumosorosea with different pathogenicity were carried out using light, scanning, and transmission electron microscopy. We found that C. fumosorosea tended to adhere to the cuticle surfaces containing protrusions. Although conidia of the lower pathogenic strain IFCF-D58 germinated, they failed to penetrate and complete the development cycle. In contrast, the higher pathogenic strain IFCF01 began to germinate within 4 h and attached to the cuticle by a thin mucilaginous matrix within 8 h post-inoculation. After 24 h post-inoculation, germ tubes and penetrating hyphae reached the cuticular epidermis and began to enter the haemocoel. Within 36 h post-inoculation, the hyphal bodies colonized the body cavity. Hyphae penetrated from inside to outside of the body after 48 h and sporulated the cadavers. After 72 h post-inoculation, numerous conidia emerged and the mycelial covered the entire cuticular surface. The two strains showed similarities in terms of conidial size and germination rate. However, IFCF-D58 exhibited significantly fewer appressoria and longer penetrating hyphae compared to the more infective IFCF01 on all surface topographies. The current pathogen invasion sequence of events suggested that the aggressive growth and propagation along with rapid and massive in vivo production of blastospores facilitate the conidia of IFCF01 to quickly overcome the diamondback moth’s defense mechanism.
The SADDEN DEATH Study: Results from a Pilot Study in Non-ICU COVID-19 Spanish Patients
Authors: Pérez-García Carlos Nicolás , Enríquez-Vázquez Daniel , Méndez-Bailón Manuel , Olmos Carmen , Gómez-Polo Juan Carlos , Iguarán Rosario , Ramos-López Noemí , García-Klepzig José Luis , Ferrández-Escarabajal Marcos , Jerónimo Adrián , Martínez-Gómez Eduardo , Font-Urgelles Judit , Fragiel-Saavedra Marcos , Paz-Arias Pilar , Romero-Delgado Teresa , Gómez-Álvarez Zaira , Playán-Escribano Julia , Jaén Esther , Vargas Gianna , González Elizabeth , Orviz Eva , Burruezo Irene , Calvo Alberto , Nieto Ángel , Molino Ángel , Lorenzo-Villalba Noël , Andrès Emmanuel , Macaya Carlos , Vilacosta Isidre
Published: February 2021

Introduction: The worldwide pandemic, coronavirus disease 2019 (COVID-19) is a novel infection with serious clinical manifestations, including death. Our aim is to describe the first non-ICU Spanish deceased series with COVID-19, comparing specifically between unexpected and expected deaths. Methods: In this single-centre study, all deceased inpatients with laboratory-confirmed COVID-19 who had died from March 4 to April 16, 2020 were consecutively included. Demographic, clinical, treatment, and laboratory data, were analyzed and compared between groups. Factors associated with unexpected death were identified by multivariable logistic regression methods. Results: In total, 324 deceased patients were included. Median age was 82 years (IQR 76–87); 55.9% males. The most common cardiovascular risk factors were hypertension (78.4%), hyperlipidemia (57.7%), and diabetes (34.3%). Other common comorbidities were chronic kidney disease (40.1%), chronic pulmonary disease (30.3%), active cancer (13%), and immunosuppression (13%). The Confusion, BUN, Respiratory Rate, Systolic BP and age ≥65 (CURB-65) score at admission was >2 in 40.7% of patients. During hospitalization, 77.8% of patients received antivirals, 43.3% systemic corticosteroids, and 22.2% full anticoagulation. The rate of bacterial co-infection was 5.5%, and 105 (32.4%) patients had an increased level of troponin I. The median time from initiation of therapy to death was 5 days (IQR 3.0–8.0). In 45 patients (13.9%), the death was exclusively attributed to COVID-19, and in 254 patients (78.4%), both COVID-19 and the clinical status before admission contributed to death. Progressive respiratory failure was the most frequent cause of death (92.0%). Twenty-five patients (7.7%) had an unexpected death. Factors independently associated with unexpected death were male sex, chronic kidney disease, insulin-treated diabetes, and functional independence. Conclusions: This case series provides in-depth characterization of hospitalized non-ICU COVID-19 patients who died in Madrid. Male sex, insulin-treated diabetes, chronic kidney disease, and independency for activities of daily living are predictors of unexpected death.
Agonism of Prostaglandin E2 Receptor 4 Ameliorates Tubulointerstitial Injury in Nephrotoxic Serum Nephritis in Mice
Authors: Aringer Ida , Artinger Katharina , Schabhüttl Corinna , Bärnthaler Thomas , Mooslechner Agnes A. , Kirsch Andrijana , Pollheimer Marion , Eller Philipp , Rosenkranz Alexander R. , Heinemann Akos , Eller Kathrin
Published: February 2021

Selectively targeting the E-type prostanoid receptor 4 (EP4) might be a new therapeutic option in the treatment of glomerulonephritis (GN), since the EP4 receptor is expressed on different immune cells, resident kidney cells, and endothelial cells, which are all involved in the pathogenesis of immune-complex GN. This study aimed to evaluate the therapeutic potential and to understand the mode of action of EP4 agonist in immune-complex GN using the murine model of nephrotoxic serum nephritis (NTS). In vivo, NTS mice were treated two times daily with two different doses of an EP4 agonist ONO AE1-329 or vehicle for 14 days total. The effect of PGE2 and EP4 agonism and antagonism was tested on murine distal convoluted tubular epithelial cells (DCT) in vitro. In vivo, the higher dose of the EP4 agonist led to an improved NTS phenotype, including a reduced tubular injury score and reduced neutrophil gelatinase-associated lipocalin (NGAL) and blood urea nitrogen (BUN) levels. EP4 agonist treatment caused decreased CD4+ T cell infiltration into the kidney and increased proliferative capacity of tubular cells. Injection of the EP4 agonist resulted in dose-dependent vasodilation and hypotensive episodes. The low-dose EP4 agonist treatment resulted in less pronounced episodes of hypotension. In vitro, EP4 agonism resulted in cAMP production and increased distal convoluted tubular (DCT) proliferation. Taken together, EP4 agonism improved the NTS phenotype by various mechanisms, including reduced blood pressure, decreased CD4+ T cell infiltration, and a direct effect on tubular cells leading to increased proliferation probably by increasing cAMP levels.
Antibody-Oligonucleotide Conjugates: A Twist to Antibody-Drug Conjugates
Authors: Dugal-Tessier Julien , Thirumalairajan Srinath , Jain Nareshkumar
Published: February 2021

A summary of the key technological advancements in the preparation of antibody–oligonucleotide conjugates (AOCs) and the distinct advantages and disadvantages of AOCs as novel therapeutics are presented. The merits and demerits of the different approaches to conjugating oligonucleotides to antibodies, antibody fragments or other proteins, mainly from the perspective of AOC purification and analytical characterizations, are assessed. The lessons learned from in vitro and in vivo studies, especially the findings related to silencing, trafficking, and cytotoxicity of the conjugates, are also summarized.