bluuebunny/crossref_metadata_2025 huggingface dataset:
CXCL12/SDF-1 in IgG4-Related Disease
Authors: Capecchi Riccardo , Croia Cristina , Puxeddu Ilaria , Pratesi Federico , Cacciato Andrea , Campani Daniela , Boggi Ugo , Morelli Luca , Tavoni Antonio , Migliorini Paola
Published: October 2021

Background: SDF-1/CXCL12 is a chemokine with pleiotropic functions in hematopoietic stem cell niche homeostasis, germinal center architecture, B cell maturation, neoangiogenesis, and fibrosis. Recently, the CXCL12/CXCR4/CXCR7 axis was associated with cancer metastasis and autoimmune diseases. The IgG4-related disease (IgG4-RD) is a pathological condition characterized by IgG4+ plasma cells infiltrating fibrotic lesions. The aim of this research is to investigate the relevance of SDF-1/CXCL12 in IgG4-RD.

Materials and Methods: Peripheral blood samples were collected before therapy from a single-center cohort of 28 IgG4-RD patients, fulfilling the ACR-EULAR classification criteria. Clinical and serological data were obtained for each patient. In total, 14 healthy donors (NHS), 9 patients with pancreatic ductal adenocarcinoma (PDAC), and 9 with Sjogren syndrome (SSj) were recruited as controls and screened for circulating SDF-1/CXCL12 by ELISA. Moreover, paraffin-embedded pancreatic biopsies obtained from patients with IgG4-RD (n = 7), non-autoimmune pancreatitis (n = 3), PDAC (n = 5), and control tissues (n = 4) were analyzed to study the tissue expression and localization of SDF-1/CXCL12 and one of its receptors, CXCR4, and their potential relation with neutrophil extracellular traps (NETs).

Results: IgG4-RD patients had higher serum levels of SDF-1/CXCL12 than normal controls (p = 0.0137). Cytokine levels did not differ between the IgG4-RD autoimmune pancreatitis (AIP) and retroperitoneal fibrosis nor between the single- and multiple-organ involvement. No correlation was seen with the IgG4-RD Responder Index, IgG4 levels, white blood cells, or inflammatory markers in the serum. When compared to SSj, the IgG4-RD AIP subgroup presents higher amounts of serum SDF-1/CXCL12 (p = 0.0275), while no differences are seen in comparison with PDAC. The expression of SDF-1/CXCL12 in the tissue was significantly higher in the IgG4-RD tissue than the normal pancreas, and the tissue with the high SDF-1/CXCL12 expression is characterized by the overall inflammatory cell infiltration, fibrosis, and high level of NETs.

Conclusion: Modulating B cell development, neoangiogenesis and fibrosis, and SDF-1/CXCL12 may play a role in IgG4-RD. The higher levels observed in IgG4-RD, as compared to SSj, which closely mimics the disease, can be related to a different pattern of lesions, with prevalent fibrosis seen in IgG4-RD. Taken together, these findings suggest that drugs acting on the CXCL12/CXCR4/CXCR7 axis may affect IgG4-RD.
Educational Psychology-Based Strategy for Instrumental Music Teaching in Normal College
Authors: Li Yang
Published: October 2021

The study is intended to explore the teaching mode of instrumental music teaching in normal college, so as to have qualified instrumental music teaching talents. Based on relevant content of educational psychology, the current instrumental music teaching model of normal college should be reformed in terms of curriculum content, teaching mode, and teaching methods, and a more systematic and standardized education model should be established. As per Orff’s music teaching method, a music recommendation model is established based on the convolutional neural network model to provide students with a positive and happy learning environment for instrumental music, and music materials that meet their personal preferences and performance level through user data. The outcomes show that the designed music recommendation model has a music recommendation accuracy rate of 0.3 and a recall rate of 0.29 when the recommendation list is 30, which conforms to the general rules of the music recommendation system. The study is expected to provide reference for establishment of a standardized and systematic instrumental music teaching strategy in normal college.
The Moderating Role of Empathy Profiles in the Relationship Between Knowledge About Aging and Attitudes Toward Older Adults Among Nursing Students
Authors: Yao Gui-Ying , Luo Yan-Yan , Zhao Zhi-Min , Zhu Bo , Gao Min
Published: October 2021

Knowledge about aging (KA) and empathy affect nursing students’ attitudes toward older adults. However, little is known about the mechanisms underlying this phenomenon from an integrated, person-centered perspective. The purposes of the present study were (1) to identify empathy profiles based on the Interpersonal Reactivity Index (IRI) among Chinese nursing students and (2) to explore whether these latent empathy profiles moderate the association between KA and attitudes toward older people. A cross-sectional survey design was used, and a battery of questionnaires – including those on demographic information, the Chinese version of Palmore’s Facts on Aging Quiz (C-FAQ), the Chinese version of Kogan’s Attitude Toward Older People Scale (C-KAOP), and the IRI – was filled in by 622 Chinese nursing students (Mage 21.76; SD = 1.33). The mean total scores on KAOP and C-FAQ were 164.96 ± 18.32 and 10.436 ± 3.015, respectively, indicating relatively positive attitudes toward older people but low KA among Chinese nursing students. Latent profile analysis was used to identify a three-profile solution characterized by distinct levels of four dimensions of empathy, namely average empathy (AE, n = 399), high empathy (HE, n = 42), and low empathy (LE, n = 181). Subsequent linear regression analysis revealed that the LE rather than the HE profile predicted positive attitudes toward older adults. It is worth noting that the LE profile played a remarkable moderating role in associations between KA and negative attitudes toward older adults after controlling for covariant variables. Both the identification of distinct empathy profiles and the interplay between the LE profile and KA are of significance in reducing negative attitudes toward older adults among Chinese nursing students. Nursing educators should combine improving nursing students’ levels of KA and fostering greater empathy to reduce negative attitudes toward older adults. Such training should give priority to nursing students with LE.
Posttraumatic Growth and Coping Strategies Among Patients With Head and Neck Cancer: Do Approach Coping and Avoidant Coping Predict Posttraumatic Growth Over Time?
Authors: Nik Jaafar Nik Ruzyanei , Abd Hamid Norhaliza , Hamdan Nur Amirah , Rajandram Rama Krsna , Mahadevan Raynuha , Mohamad Yunus Mohd Razif , Zakaria Hazli , Leong Bin Abdullah Mohammad Farris Iman
Published: October 2021

Despite an enormous number of studies addressing the importance of posttraumatic growth (PTG) among cancer patients, the literature lacks data regarding how different coping strategies affect PTG among head and neck cancer (HNC) patients over time. This longitudinal study investigated the PTG trend and coping over 5–7months among a cohort of HNC patients within the first year after their diagnosis. It determined an association between coping strategies and PTG over time. The study’s HNC respondents were administered a socio-demographic and clinical characteristics questionnaire during their baseline assessments. Additionally, the Malay versions of the “PTG Inventory-Short Form” (PTGI-SF) and the “Brief Coping Orientation to Problems Experienced Inventory” (Brief COPE) were administered during respondents’ baseline assessments and follow-up assessments (5–7months after the baseline assessments). In total, 200 respondents reported an increasing PTG trend and approach coping (active coping, planning, positive reframing, acceptance, emotional support, and instrumental support) and a decreasing trend of avoidant coping (self-distraction and denial) over time. Two approach coping strategies (acceptance and planning) significantly increased PTG while denial was the only avoidant coping strategy that significantly lowered PTG, after controlling for socio-demographic and clinical characteristics, over time. Our study’s findings identified the need to incorporate psychosocial interventions that enhance approach coping and reduce avoidant coping into HNC patients’ treatment regimes.
Development and Psychometric Properties of the Synthetic Drug Dependence Scale in a Chinese Sample
Authors: Li Mei-Ting , Zhang Jun , Zhang Dong-Cheng , Che Qing-Qing , Liu Ze-Lan , Yang Pei-Wen , Luo Xin-Wei , Cai Tai-Sheng
Published: October 2021

Objective: In contrast to the drug situation in the rest of the world, synthetic drugs, rather than traditional drugs, have been the dominant abused drugs in China since 2019. However, the public misconception that synthetic drugs are not as addictive as traditional drugs, such as opioids and the scarcity of specific measurement instruments, have hindered the clinical diagnosis and treatment of synthetic drug abusers, thus the development of a localized instrument to evaluate dependence on synthetic drugs is in urgently needed.

Method: Using a sample of 618 Chinese synthetic drug abusers (Mean age = 34.69 years; 44.17% female), the present study developed and examined the psychometric properties of a self-reporting instrument, the Synthetic Drug Dependence Scale (SDDS), which consists of four subscales: physical dependence, psychological dependence, health injury, and social function injury.

Results: The SDDS revealed a three-factor model structure (weighted root mean square residual (WRMR) = 0.876, comparative fit index (CFI) = 0.965, Tucker–Lewis index (TLI) = 0.953, and Root mean square error of approximation (RMSEA) = 0.070), with good internal consistency (composite reliability = 0.912, alfa = 0.801) and convergent validity. Elevated scores on the SDDS were associated with a higher level of reward sensitivity, punishment sensitivity, and stronger impulsivity. Interestingly, psychological dependence was the only significant predictor (p < 0.05) of criterion variables compared with the other three subscales, implying the important role of psychological factors in synthetic drugs dependence. Adequate measurement equivalence across sex, age (18–30 and 31–57 years old), and employment group (employed and unemployed) was also established.

Conclusion: The SDDS appears to be an effective and reliable instrument that could be used to further investigate the characteristics of synthetic and traditional drug dependence, promoting a deeper understanding of the physical and psychological roles in drug dependence.
Discourse-Level Information Recall in Early and Late Bilinguals: Evidence From Single-Language and Cross-Linguistic Tasks
Authors: Chou Isabelle , Hu Jiehui , Muñoz Edinson , García Adolfo M.
Published: October 2021

Bilingualism research indicates that verbal memory skills are sensitive to age of second language (L2) acquisition (AoA). However, most tasks employ disconnected, decontextualized stimuli, undermining ecological validity. Here, we assessed whether AoA impacts the ability to recall information from naturalistic discourse in single-language and cross-linguistic tasks. Twenty-four early and 25 late Chinese-English bilinguals listened to real-life L2 newscasts and orally reproduced their information in English (Task 1) and Chinese (Task 2). Both groups were compared in terms of recalled information (presence and correctness of idea units) and key control measures (e.g., attentional skills, speech rate). Across both tasks, information completeness was higher for early than late bilinguals. This occurred irrespective of attentional speed, speech rate, and additional relevant factors. Such results bridge the gap between classical memory paradigms and ecological designs in bilingualism research, illuminating how particular language profiles shape information processing in daily communicative scenarios.
Palestinian and Norwegian Kindergarten Teachers' Perspectives on Psychosocial Support: A Qualitative Study
Authors: El-Khodary Basel , Christensen Ingrid , Abou-Dagga Sanaa , Raji Shawqi , Lyden Susan
Published: October 2021

The current qualitative case study aims to explore and map the concepts and the conditions for providing psychosocial support in kindergarten across two vastly different countries, Palestine and Norway. The global challenge of providing psychosocial support toward children is increasingly acknowledged. Although education is described as crucial for psychosocial support from the health sector, studies dealing with the educational perspective on this topic are rare. Data from 26 participants (10 from Gaza, 10 from Hebron and 6 from Norway) were collected in qualitative semi-structured interviews. Despite vastly different contexts, the analysis showed some important common features. Kindergarten teachers in both countries link psychosocial support conceptually to psychological and emotional knowledge. The teachers in both countries are concerned about the psychosocial support being performed repeatedly in everyday situations, such as establishing routines, paying extra attention, and calming children and creating everyday safe spaces. They give detailed descriptions of the quality of their long-term, yet professional relations with the child. Time and space are crucial challenges in both countries, and they call for more knowledge on mental health. A main difference between the two countries was the role of the community and relation to parents. The Palestinian teachers defined psychosocial support as a “set of community services,” the teachers were frustrated with the lack of parental collaboration. The Norwegian teachers downscaled or overlooked the importance of community or parents and community. The findings give overall presentations of the concepts and the conditions for providing psychosocial support in education Palestine and Norway. We argue that education not only represents sites for conducting health-directed interventions but represents important resources for developing the field of psychosocial support in collaboration with community services. Education – and especially kindergarten provides other values, knowledge, and structural resources for the development programs and knowledge on psychosocial support.
Yale Global Tic Severity Scale (YGTSS): Psychometric Quality of the Gold Standard for Tic Assessment Based on the Large-Scale EMTICS Study
Authors: Haas Martina , Jakubovski Ewgeni , Fremer Carolin , Dietrich Andrea , Hoekstra Pieter J. , Jäger Burkard , Müller-Vahl Kirsten R. ,   The EMTICS Collaborative Group
Published: October 2021

The Yale Global Tic Severity Scale (YGTSS) is a clinician-rated instrument considered as the gold standard for assessing tics in patients with Tourette's Syndrome and other tic disorders. Previous psychometric investigations of the YGTSS exhibit different limitations such as small sample sizes and insufficient methods. To overcome these shortcomings, we used a subsample of the large-scale “European Multicentre Tics in Children Study” (EMTICS) including 706 children and adolescents with a chronic tic disorder and investigated convergent, discriminant and factorial validity, as well as internal consistency of the YGTSS. Our results confirm acceptable convergent and good to very good discriminant validity, respectively, indicated by a sufficiently high correlation of the YGTSS total tic score with the Clinical Global Impression Scale for tics (rs = 0.65) and only low to medium correlations with clinical severity ratings of attention deficit/hyperactivity symptoms (rs = 0.24), obsessive–compulsive symptoms (rs = 27) as well as internalizing symptoms (rs = 0.27). Internal consistency was found to be acceptable (Ω = 0.58 for YGTSS total tic score). A confirmatory factor analysis supports the concept of the two factors “motor tics” and “phonic tics,” but still demonstrated just a marginal model fit (root mean square error of approximation = 0.09 [0.08; 0.10], comparative fit index = 0.90, and Tucker Lewis index = 0.87). A subsequent analysis of local misspecifications revealed correlated measurement errors, suggesting opportunities for improvement regarding the item wording. In conclusion, our results indicate acceptable psychometric quality of the YGTSS. However, taking the wide use and importance of the YGTSS into account, our results suggest the need for further investigations and improvements of the YGTSS. In addition, our results show limitations of the global severity score as a sum score indicating that the separate use of the total tic score and the impairment rating is more beneficial.
Comorbidity and Treatment in Older Psychiatric In-patients—A Retrospective Study in a Chinese Psychiatric Hospital
Authors: Liu Hongmei , Zhu Yuncheng , Wu Xiaohui , He Kan , Wang Xiaoxiao , Sun Ping , Zhao Jie , Yao Yamin , Ren Juanjuan , Mao Ruizhi , Yang Tao , Yang Lu , Sun Xiujia , Jiang Ping , Zhang Chen , Fang Yiru
Published: October 2021

Background: Comorbid somatic diseases increase the death risk and affect the condition, treatment, and prognosis of older psychiatric patients. We investigated the comorbidity and drug treatment in older patients with psychosis.

Methods: This retrospective study used data from 3,115 older psychiatric in-patients hospitalized at the Shanghai Mental Health Center Affiliated to Shanghai Jiaotong University School of Medicine, China discharged from 2005 to 2015. Descriptive analyses of patients' age, sex, treatment drugs, diagnoses (based on ICD-10), and time trend were performed.

Results: Patients' median age was 56 (range, 50-98) years; 1,824 (58.6%) were female. The top five first-level diagnoses were schizophrenia (F20) (n = 1,818, 58.3%), depressive episode (F32) (n = 457, 14.6%), bipolar affective disorder (F31) (n = 151, 4.8%), manic episode (F30), (n = 143, 4.6%), and vascular dementia (F01) (n = 136, 4.4%). Mental (99.9%), central nervous system (85.2%), digestive system (83.5%), cardiovascular system (72.5%), and anti-infective (59.6%) drugs had the highest prescription rates. The combined use of antidepressants, anti-anxiety, anti-arrhythmic, hormones and endocrine system drugs were significantly higher in female than in male patients, while mood stabilizers and genitourinary system drugs significantly more frequent in men. With increasing age, the F20-F29 patients decreased, while F00-F09 patients increased, with the corresponding changes to prescription in those patients. In comparison to that in 2005-2010, the combined prescriptions for genitourinary and cardiovascular drugs increased between 2011 and 2015, and F00-F09 and F40-F48 older patients doubled, accordingly anti-Alzheimer's disease drugs and antidepressants more than doubled. F30-F39 patients increased by 49.1%, and anti-anxiety drugs, mood stabilizers, etc. increased by ≥50%; F20-F29 older patients decreased by 26.7%, while antipsychotics only increased by 4.4%.

Conclusions: This study found the combined drug treatment of somatic diseases, particularly for central nervous, digestive, cardiovascular, respiratory and genitourinary drugs were extremely common among older psychiatric in-patients in China. With increasing age, the F20-F29 patients decreased, while F00-F09 patients increased; the antipsychotics prescriptions decreased, and almost all comorbidity drugs increased. Compared with that in 2005-2010, the older patients with all diagnosis except F20-F29 increased in 2011-2015, and the prescriptions for psychotropic, genitourinary, and cardiovascular drugs increased.
Mental Health Disorders in Nurses During the COVID-19 Pandemic: Implications and Coping Strategies
Authors: Riedel Brittney , Horen Sydney R. , Reynolds Allie , Hamidian Jahromi Alireza
Published: October 2021

Nurses caring for patients who contract coronavirus disease 2019 (COVID-19) have experienced significant traumas in the form of increased workloads, negative patient outcomes, and less social support system access. Nurses should be provided with information regarding early detection, coping skills and treatment for anxiety, depression, post-traumatic stress syndrome (PTSS)/post-traumatic stress disorder (PTSD), and other mental health disorders. Early intervention is important as mental health disorders can cause dysfunction, internal suffering, and in the most extreme situations, lead to death if not properly cared for. Healthcare corporations should consider providing coverage for mental health treatment for employees who experience COVID-19 traumas. With the implementation of healthy coping skills and therapeutic intervention, nurses will be able to let go of the negative impacts that the COVID-19 pandemic has caused and reintegrate into their roles as caring and entrusted health care providers. The current paper evaluates the mental health disorders encountered by nurses in the COVID-19 era based on the current medical literature and aims to provide practical coping strategies.
Challenges of Developing Robust AI for Intrapartum Fetal Heart Rate Monitoring
Authors: O’Sullivan M. E. , Considine E. C. , O'Riordan M. , Marnane W. P. , Rennie J. M. , Boylan G. B.
Published: October 2021

Background:CTG remains the only non-invasive tool available to the maternity team for continuous monitoring of fetal well-being during labour. Despite widespread use and investment in staff training, difficulty with CTG interpretation continues to be identified as a problem in cases of fetal hypoxia, which often results in permanent brain injury. Given the recent advances in AI, it is hoped that its application to CTG will offer a better, less subjective and more reliable method of CTG interpretation.

Objectives:This mini-review examines the literature and discusses the impediments to the success of AI application to CTG thus far. Prior randomised control trials (RCTs) of CTG decision support systems are reviewed from technical and clinical perspectives. A selection of novel engineering approaches, not yet validated in RCTs, are also reviewed. The review presents the key challenges that need to be addressed in order to develop a robust AI tool to identify fetal distress in a timely manner so that appropriate intervention can be made.

Results:The decision support systems used in three RCTs were reviewed, summarising the algorithms, the outcomes of the trials and the limitations. Preliminary work suggests that the inclusion of clinical data can improve the performance of AI-assisted CTG. Combined with newer approaches to the classification of traces, this offers promise for rewarding future development.
Reconfigurable Intelligent Surface-Assisted Bluetooth Low Energy Link in Metal Enclosure
Authors: Lodro Mir , Gradoni Gabriele , Gros Jean-Baptiste , Greedy Steve , Lerosey Geoffroy
Published: October 2021

Reconfigurable intelligent surface (RIS) technology is at the forefront for its transformative role in future wireless communication systems such as wireless local area networks (WLAN), sixth-generation (6G) communication, and internet-of-things (IoT). This paper presents RIS-assisted Bluetooth low energy (BLE) communication links in neighbor discovery mode. We optimized the packet error rate (PER) performance of the BLE communication link in a highly reflecting metal enclosure environment. We used one RIS for the PER optimization of four BLE physical (PHY) modes. Then, we used two RISs simultaneously in a distributed and centralized manner to further optimize the PER of all BLE PHY modes. We found PER optimization using two RISs is better than the PER optimization using one RIS. Additionally, PER optimization using a centralized arrangement of RISs outperformed PER optimization using distributed arrangement. We found the coded BLE modes i.e., LE500K and LE125K show lower PER than the uncoded counterpart i.e., LE1M and LE2M. This is because uncoded BLE PHY modes have higher data rates than the coded BLE PHY modes. Because of additional channel power gains introduced by RIS-based passive beamforming, the PER of coded and uncoded BLE PHY modes is further reduced.
Endometriosis and Uterine Fibroids (Leiomyomata): Comorbidity, Risks and Implications
Authors: Uimari Outi , Nazri Hannah , Tapmeier Thomas
Published: October 2021

Uterine Fibroids (leiomyomata) and endometriosis affect millions of women world-wide. Although aetiology and natural history of the conditions are markedly different, symptoms can overlap and make differential diagnoses necessary, often using invasive methods such as laparoscopy. Considerable comorbidity exists between the two conditions and needs to be taken into account when treating fibroids and/or endometriosis. The genetic foundations of both uterine fibroids and endometriosis remain to be fully understood but recent evidence suggest common underpinnings. Here, we discuss the comorbidity of uterine fibroids and endometriosis and the implications for diagnosis, treatment and risks.
The Importance of Monitoring River Water Discharge
Authors: Depetris P. J.
Published: October 2021

River discharge time series, originally recorded to anticipate floods and water scarcity, later became indispensable to design hydroelectric dams. Presently, discharge monitoring aids in detecting climatic and environmental change, because the discharge and quality of river water are functions of many climatic, biological, geological, and topographic variables coexisting in the basin. Climate change is altering the atmospheric precipitation distribution pattern-both, in time and space-as well as the occurrence of extreme climatic events. It is important the global upgrading of river gauging networks to unveil hydrological trends and changing atmospheric patterns. In so doing, discharge monitoring stations–and the resulting time series-may be, as well, invaluable in revealing the role played by significant environmental variables.
“Non-Triangle Plane” Surgical Technique of Video-Assisted Thoracic Surgery Atypical Segmentectomy for Stage IA Non-Small-Cell Lung Cancer: Early Experience
Authors: Zhou Chao , Qian Jun , Li Wentao
Published: October 2021

Objectives: To evaluate the safety and feasibility of a novel surgical technique (“non-triangle plane” technique) of two-port (mini-utility) video-assisted thoracic surgery (VATS) atypical segmentectomy (S3+S1+2c) with tunneling stapler for small-sized non-small-cell lung cancers (NSCLCs) located in left S3 close to the intersegmental plane between S3 and S1+2c.

Materials and Methods: This retrospective descriptive study included 16 patients who, between April 2016 and December 2019, underwent a single two-port (mini-utility) VATS atypical segmentectomy (S3+S1+2c) with tunneling stapler technique for small-sized NSCLCs with a ground-glass opacity (GGO) rate of more than 50% by a constant surgical team in two hospitals. Perioperative data and survival data were collected and retrospectively analyzed. Postoperative follow-up was performed every 6 months.

Results: Six patients were with adenocarcinoma in situ, and ten were with minimally invasive adenocarcinoma. The mean surgical margin was 14.06 ± 3.02 mm. The mean operation time was 53.88 ± 9.76 min. The mean duration of chest tube drainage was 4 ± 1.21 days, and the median length of postoperative hospital stay was 4 days. There was no perioperative morbidity and mortality. The median follow-up was 47.5 months (17–61 months). No recurrences occurred, and all patients were still alive at the last registered follow-up (May 31, 2021).

Conclusion: Two-port (mini-utility) VATS atypical segmentectomy (S3+S1+2c) with tunneling stapler technique is a safe and feasible option for the treatment of small-sized NSCLCs located in left S3 close to the intersegmental plane between S3 and S1+2c.
Case Report: Primary Bilateral Minimally Invasive Adenocarcinoma of the Lungs in an 11-Year-Old Child: A Rare Case
Authors: Lei Xing , Zheng Yongfei , Zhang Guohua , Zheng Hailan
Published: October 2021

There are many types of benign and malignant tissue, but primary lung tumor is very rare in children and often remains undiagnosed until after distant metastasis has occurred. Few cases of early lung adenocarcinoma in children have been reported. However, this case concerns an 11-year-old child with primary bilateral minimally invasive adenocarcinoma. As far as we know, this is the youngest reported case of its type.
Effects of Slaughter Age on Myosin Heavy Chain Isoforms, Muscle Fibers, Fatty Acids, and Meat Quality in Longissimus Thoracis Muscle of Tibetan Sheep
Authors: Bao Gaoliang , Liu Xiu , Wang Jiqing , Hu Jiang , Shi Bingang , Li Shaobin , Luo Yuzhu
Published: October 2021

Tibetan sheep is one of the dominant livestock at Qinghai-Tibet Plateau, which is the main food source of local people. In order to investigate the effect of slaughter age on meat quality, fatty acid profile and expression of myosin heavy chain (MyHC) isoform genes were analyzed in Tibetan sheep. A total of 24 Tibetan sheep including 4 months old (4 m), 1.5 years old (1.5 y), 3.5 years old (3.5 y), and 6 years old (6 y) were randomly selected. The results indicated that the MyHC IIx and MyHC IIb mRNAs increased with age, whereas MyHC IIa mRNA decreased. MyHC I mRNA was highest at 3.5 y. There were differences in the muscle fiber types of Tibetan sheep at different ages. Intramuscular fat (IMF) was highest at 1.5 y, the pH45min and pH24h value of 6 y sheep were lower than the other groups, the shear force increased with age (p < 0.05), and drip loss increased with age (p < 0.01). Tibetan sheep at 1.5 y had lower saturated fatty acid (SFA) contents and higher monounsaturated fatty acid (MUFA) contents (p < 0.05). Different muscle fiber types influence the meat quality and fatty acid composition of Tibetan sheep with increasing age. These results demonstrated the effect of age on meat quality of Tibetan sheep through regulation of expression of the MyHC isoforms which changed the myofiber types, and 1.5 y Tibetan sheep meat was more suitable for a healthy human diet.
A Computational Reverse Vaccinology Approach for the Design and Development of Multi-Epitopic Vaccine Against Avian Pathogen Mycoplasma gallisepticum
Authors: Mugunthan Susithra Priyadarshni , Mani Chandra Harish
Published: October 2021

Avian mycoplasma is a bacterial disease causing chronic respiratory disease (CRD) in poultry industries with high economic losses. The eradication of this disease still remains as a challenge. A multi-epitope prophylactic vaccine aiming the antigenic proteins of Mycoplasma gallisepticum can be a capable candidate to eradicate this infection. The present study is focused to design a multi-epitope vaccine candidate consisting of cytotoxic T-cell (CTL), helper T-cell (HTL), and B-cell epitopes of antigenic proteins, using immunoinformatics strategies. The multi-epitopic vaccine was designed, and its tertiary model was predcited, which was further refined and validated by computational tools. After initial validation, molecular docking was performed between multi-epitope vaccine construct and chicken TLR-2 and 5 receptors, which predicted effective binding. The in silico results specify the structural stability, precise specificity, and immunogenic response of the designed multi-epitope vaccine, and it could be an appropriate vaccine candidate for the M. gallisepticum infection.
Aberrant Expression of β-Catenin Correlates with Infiltrating Immune Cells and Prognosis in NSCLC
Authors: Zheng Hongmei , Ning Yue , Yang Yang , Zhan Yuting , Wang Haihua , Wen Qiuyuan , Peng Jinwu , Fan Songqing
Published: October 2021

Aims: β-catenin is a critical regulating factor of the Wnt pathway, which is closely linked to tumorigenesis, tumor growth, metastasis, and tumor immunity. Our study focused on exploring the relationship between β-catenin and clinicopathological features, prognosis, as well as infiltrating immune cells and immune scores, so as to illustrate its clinical significance in NSCLC.

Materials and Methods: The β-catenin mRNA (CTNNB1) and protein expression data were downloaded from the UALCAN and the UCSC Xena website, respectively. All tumor-immune infiltrating cells’ data were downloaded from the TIMER platform and immune scores were downloaded from ESTIMATE website. The expression of β-catenin protein in our cohort was measured by immunohistochemistry.

Results: β-catenin mRNA level was higher in lung adenocarcinoma (LUAD) compared to normal tissues (p < 0.001) and was related to overall survival (OS) (p < 0.001) and post-progression survival (PPS) (both p = 0.049) in LUAD. Aberrant β-catenin protein expression was higher in male and lung squamous cell carcinoma (LUSC) patients (both p = 0.001). Also, it was considered to be a prognosis factor independently (p = 0.034). In addition, β-catenin protein was negatively correlated with CD8+T cells (r = −0.128, p = 0.008), neutrophils (r = −0.198, p < 0.001), immune score (r = −0.109, p = 0.024), stromal score (r = −0.097, p = 0.045), and ESTIMATE score (r = −0.113, p = 0.020).

Conclusions: Aberrant β-catenin protein expression was evidently higher in NSCLC and might serve as a biomarker for poor prognosis. Most importantly, β-catenin protein might play an important part in tumor immunity and the tumor microenvironment by inhibiting the infiltration of CD8+ T cells and neutrophils.
Alleviating Soil Acidification and Increasing the Organic Carbon Pool by Long-Term Organic Fertilizer on Tobacco Planting Soil
Authors: Dai Peigang , Cong Ping , Wang Peng , Dong Jianxin , Dong Zhaorong , Song Wenjing
Published: October 2021

Long term tobacco planting leads to soil acidification. A ten-year experiment with various fertilization treatments (no fertilization (CK), chemical fertilizer (CF), organic-inorganic compound fertilizer (OCF), and organic fertilizer (OF)) was carried out between 2010 and 2020 in a continuous cropping system of Nicotiana tabacum in the brown soil of eastern China, to assess the effects of organic fertilizer on the improvement of tobacco planting soil acidification. The results indicated that treatments OCF and OF reduced the soil exchangeable acid content, of which the exchangeable aluminum showed the largest reduction by 51.28% with the OF treatment. In contrast, treatment CF showed more significant increases in exchangeable aluminum (Al) and Al saturation, and also apparently increased soil NO3−-N, NH4+-N and nitrification potential (NP) than other treatments. Treatments of OCF and OF significantly increased the total amount of exchangeable base (EBC) by 37.19% and 42.00% compared with CF, respectively. Redundancy analysis (RDA) showed that NP, NH4+-N, and NO3−-N were the important factors indicating soil acidification, while EBC and exchangeable K were the significant factors restricting soil acidification. Inevitably, OCF could improve the soil organic carbon pool and labile organic carbon pool. The structural equation model (SEM) showed that OCF treatment increased the soil organic carbon pool mainly by inhibiting soil nitrification and reducing the content of exchangeable Al. In conclusion, both OF and OCF treatments were effective methods to alleviate tobacco planting soil acidification, however OCF had more advantages in improving soil organic carbon pool.
Dietary Resin Acid Concentrate Improved Performance of Broiler Chickens and Litter Quality in Three Experiments
Authors: Lipiński Krzysztof , Vuorenmaa Juhani , Mazur-Kuśnirek Magdalena , Sartowska-Żygowska Katarzyna , Kettunen Hannele
Published: October 2021

Dietary coniferous resin acids have previously been suggested to support the intestinal integrity of broiler chickens by reducing mucosal collagen degradation. The present study examined the effects of resin acid concentrate (RAC) on broiler performance and litter quality. In trial 1, RAC was added to diets at 0, 125, 250, or 1250 g/ton, while in trials 2 and 3, RAC dosing was 0 or 175 g/ton. Bird weight, feed consumption, mortality, feed conversion ratio (FCR), European Efficiency Index (EEI), litter moisture, and footpad dermatitis (FPD) lesions were measured. In trial 1, RAC at 125 and 250 g/ton improved weight gain and EEI, while RAC at 1250 g/ton group did not differ from control. Feed consumption, FCR, FPD scores and mortality were similar in all treatments, but litter quality was improved by all doses of RAC. In trials 2 and 3, RAC increased the final weight of birds, improved FCR, EEI, and litter quality, but had no effects in other parameters. In summary, RAC at 125–250 g/ton improved bird performance and thus shows promise as a feed additive. The dryer litter in RAC treatments may suggest improved intestinal condition as a response to in-feed resin acids.
Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable Staphylococcus aureus Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
Authors: Gogoi-Tiwari Jully , Dorji Dorji , Tiwari Harish Kumar , Shirolkar Gayatri , Aleri Joshua W. , Mukkur Trilochan
Published: October 2021

Non-typeable (NT) Staphylococcus aureus strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT S. aureus on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT S. aureus strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the S. aureus load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (p ≤ 0.05) in those infected with the strong biofilm forming NT S. aureus strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT S. aureus infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT S. aureus and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of S. aureus advancing the time of mortality in mice warrants further investigation.
Research Concerning the Bending Properties of Reconstituted Spruce Lumber Boards, Obtained by Edge-Cutting at 45° and Gluing
Authors: Porojan Mihaela , Brandstetter Markus , Ispas Mihai , Bedelean Bogdan , Campean Mihaela
Published: October 2021

The best-possible valorization of each cut log is a priority. The aim of this research was to evaluate the potential of a solution for increasing the conversion efficiency of tapered logs, by edge-cutting the narrowest sideboards at 45° and joining them by gluing, in order to obtain usable lumber boards. The modality of obtaining the glued elements was described and the values of the density and bending properties (MOE and MOR) of these elements compared to solid wood elements taken from the same logs and the same position within each log was determined. The envisaged outcome was to determine if the bonded products are suited to be used as cores within laminated products for construction.
Exploring the Bioethanol Production Potential of Miscanthus Cultivars
Authors: Turner William , Greetham Darren , Mos Michal , Squance Michael , Kam Jason , Du Chenyu
Published: October 2021

Miscanthus is a fast-growing perennial grass that attracts significant attention for its potential application as a feedstock for bioethanol production. This report explores the difference in the lignocellulosic composition of various Miscanthus cultivars, including Miscanthus × giganteus cultivated at the same location (mainly Lincoln, UK). It also assesses the sugar release profiles and mineral composition profiles of five Miscanthus cultivars harvested over a growing period from November 2018 to February 2019. The results showed that Miscanthus × giganteus contains approximately 45.5% cellulose, 29.2% hemicellulose and 23.8% lignin (dry weight, w/w). Other cultivars of Miscanthus also contain high quantities of carbohydrates (cellulose 41.1–46.0%, hemicellulose 24.3–32.6% and lignin 21.4–24.9%). Pre-treatment of Miscanthus using dilute acid followed by enzymatic hydrolysis released 63.7–80.2% of the theoretical glucose content. Fermentation of a hydrolysate of Miscanthus × giganteus using Saccharomyces cerevisiae NCYC2592 produced 13.58 ± 1.11 g/L of ethanol from 35.13 ± 0.46 g/L of glucose, corresponding to a yield of 0.148 g/g dry weight Miscanthus biomass. Scanning electron microscopy was used to study the morphology of raw and hydrolysed Miscanthus samples, which provided visual proof of Miscanthus lignocellulose degradation in these processes. The sugar release profile showed that a consequence of Miscanthus plant growth is an increase in difficulty in releasing monosaccharides from the biomass. The potassium, magnesium, sodium, sulphur and phosphorus contents in various Miscanthus cultivars were analysed. The results revealed that these elements were slowly lost from the plants during the latter part of the growing season, for a specific cultivar, until February 2019.
Characterization of the Microstructure and Surface Roughness Effects on Fatigue Life Using the Tanaka–Mura–Wu Model
Authors: Wu Xijia , Kanz Philippe , Mahmoud Hassan , Millar Jason , Shabani Peyman , Torres Jose Martinez
Published: October 2021

Additive manufacturing (AM) has drawn tremendous interest in engineering applications because it offers almost unlimited possibilities of innovative structural design to save weight and optimize performance. However, fatigue properties are one of the limiting factors for structural applications of AM materials. The recently developed Tanaka–Mura–Wu (TMW) model is modified to include the microstructure and surface roughness factors, in addition to the material’s elastic modulus, surface energy and Burgers vector, to predict the fatigue curves as functions of stress or plastic strain for several typical AM materials as well as their conventional (wrought) counterpart. Furthermore, with statistical characterization of the microstructural effect, the model can be established to evaluate fatigue design allowables.
Quality Assessment of Dry Soil Mixing Columns in Soft Soil Areas of Eastern China
Authors: Pan Huangsong , Du Guangyin , Xia Han , Wang Haiyuan , Qin Da
Published: October 2021

To investigate the quality of dry soil mixing (DSM) columns in different soft soil areas of east China, a large number of laboratory test results and field test results of DSM columns were collected and analyzed statistically. Furthermore, a quality assessment method for DSM columns is proposed in this paper. The hardness description (HD), standard penetration test (SPT), unconfined compressive strength (UCS), and soil-cement column quality designation (SCQD) are used as assessment indexes. The statistical analysis showed that the test results of SPT, UCS, and SCQD were scattered, particularly in shallow ground. The mean values of the SPT blow count, UCS, and SCQD of the DSM columns decreased with depth: the greater the depth, the worse the quality of DSM columns. The quality assessment results showed that the proportion of the great quality columns was 64.84%, and the proportion of the unqualified columns was 1.4%. The proportion of DSM columns with great quality in the lagoon soft soil area was greater than in other areas. The proportion of unqualified columns in the lacustrine soft soil area was the largest. For all soft soil areas, the proportions of great quality, good quality, general quality, and unqualified soil decreased in that order. The quality assessment of 8627 DSM columns showed the proportions of great quality and unqualified were 64.84% and 1.4%, respectively. It was found that the greater the depth of the DSM column, the more unqualified DSM columns, and the more difficult it was to control the quality of DSM columns.
Identification of Close Modes on Frequency in Rotating Systems
Authors: Figueroa-Díaz Rafael A. , Cruz-Alcantar Pedro , Balvantín-García Antonio de J.
Published: October 2021

In the area of modal balancing, it is essential to identify the vibration modes to be balanced in order to obtain the different modal parameters that will allow knowing the correction weight and its position in the balance planes. However, in some cases, a single mode is apparently observed in the polar response diagrams used for this process, which actually contains at least two modes and which, when added vectorially, shows only one apparent mode. In these cases, in addition to the intrinsic errors when using a modal parameter extraction tool, there will be errors in determining the correction weight for the modes, as well as for the placement angle. In this work, an identification methodology is presented which, through the use of coordinate transformation and a modal parameter extraction tool, allows identifying characteristic patterns of close modes in frequency and which, when applied in the study of a system in the field, offers robustness and applicability.
A Critical Review of Unmanned Aerial Vehicles (UAVs) Use in Architecture and Urbanism: Scientometric and Bibliometric Analysis
Authors: Videras Rodríguez Marta , Melgar Sergio Gómez , Cordero Antonio Sánchez , Márquez José Manuel Andújar
Published: October 2021

In recent years the use of UAVs (Unmanned aerial vehicles) have proliferated in the civil sector for purposes such as search and rescue, remote sensing or real-time monitoring of road traffic, among others. In the architecture, engineering and construction fields (AEC) UAVs have demonstrated to be an ideal technology due to their optimal performance in terms of time, precision, safety and cost. Given the rapid growth of interest in this technology, this research presents a critical review of the literature on the use of UAVs in architecture and urbanism to define the most widely used techniques and delimit the fields of application based on the experimentation published by the scientific community. A scientific mapping was carried out in two stages using the VOSviewerTM software: a scientometric and a bibliometric analysis. This technique allowed us to analyse a large body of literature and bibliographic data to obtain trends, patterns and directions of this domain of knowledge. Then, a literature review was presented, highlighting the relevant information identified in the previous analysis. The fields of application of UAVs were delimited and the most commonly used payload types and the most appropriate post-processing techniques were specified, depending on the aerial mission objective. The fields of application identified included different techniques related to the generation of 3D models, land mapping, construction site monitoring, building surveying to detect structural damage and energy losses and urban remote sensing. The literature review showed that UAVs provide a useful multi-tasking tool at any stage of an architectural project. These techniques can be applied to buildings or public spaces from the design and construction processes when the project is initiated to the later stages of maintenance and inspection of the building during its life cycle.
Classification Schemes and Identification Methods for Urban Functional Zone: A Review of Recent Papers
Authors: Liu Baihua , Deng Yingbin , Li Miao , Yang Ji , Liu Tao
Published: October 2021

Urbanization is accelerating due to economic and societal development. The accurate identification of urban functional zones is significant for urban structure optimization, urban planning, and resource allocation. This paper reviews the scholarly literature on urban functional zone identification. Based on the retrieval results of databases, we analyzed the overview and current status. The identification methods and classification schemes are summarized from the existing research. The following results were obtained: (1) point of interest (POI) data are widely used for functional zone identification; (2) the block is the most common unit for functional zone identification; (3) cluster analysis is the main approach for urban functional zone identification; (4) most of the classification schemes are based on the dominant land use and characteristics of data sources. We predict future trends of urban functional zone identification based on the reviewed literature. Our findings are expected to be valuable for urban studies.