bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic Extraction of Recurrent Patterns of High Dominant Frequency Mapping During Human Persistent Atrial Fibrillation
Authors: Li Xin , Chu Gavin S. , Almeida Tiago P. , Vanheusden Frederique J. , Salinet João , Dastagir Nawshin , Mistry Amar R. , Vali Zakariyya , Sidhu Bharat , Stafford Peter J. , Schlindwein Fernando S. , Ng G. André
Published: March 2021

Purpose: Identifying targets for catheter ablation remains challenging in persistent atrial fibrillation (persAF). The dominant frequency (DF) of atrial electrograms during atrial fibrillation (AF) is believed to primarily reflect local activation. Highest DF (HDF) might be responsible for the initiation and perpetuation of persAF. However, the spatiotemporal behavior of DF remains not fully understood. Some DFs during persAF were shown to lack spatiotemporal stability, while others exhibit recurrent behavior. We sought to develop a tool to automatically detect recurrent DF patterns in persAF patients.

Methods: Non-contact mapping of the left atrium (LA) was performed in 10 patients undergoing persAF HDF ablation. 2,048 virtual electrograms (vEGMs, EnSite Array, Abbott Laboratories, USA) were collected for up to 5 min before and after ablation. Frequency spectrum was estimated using fast Fourier transform and DF was identified as the peak between 4 and 10 Hz and organization index (OI) was calculated. The HDF maps were identified per 4-s window and an automated pattern recognition algorithm was used to find recurring HDF spatial patterns. Dominant patterns (DPs) were defined as the HDF pattern with the highest recurrence.

Results: DPs were found in all patients. Patients in atrial flutter after ablation had a single DP over the recorded time period. The time interval (median [IQR]) of DP recurrence for the patients in AF after ablation (7 patients) decreased from 21.1 s [11.8 49.7 s] to 15.7 s [6.5 18.2 s]. The DF inside the DPs presented lower temporal standard deviation (0.18 ± 0.06 Hz vs. 0.29 ± 0.12 Hz, p < 0.05) and higher OI (0.35 ± 0.03 vs. 0.31 ± 0.04, p < 0.05). The atrial regions with the highest proportion of HDF region were the septum and the left upper pulmonary vein.

Conclusion: Multiple recurrent spatiotemporal HDF patterns exist during persAF. The proposed method can identify and quantify the spatiotemporal repetition of the HDFs, where the high recurrences of DP may suggest a more organized rhythm. DPs presented a more consistent DF and higher organization compared with non-DPs, suggesting that DF with higher OI might be more likely to recur. Recurring patterns offer a more comprehensive dynamic insight of persAF behavior, and ablation targeting such regions may be beneficial.
Plant in vitro Culture Technologies; A Promise Into Factories of Secondary Metabolites Against COVID-19
Authors: Khan Tariq , Khan Mubarak Ali , Karam Kashmala , Ullah Nazif , Mashwani Zia-ur-Rehman , Nadhman Akhtar
Published: March 2021

The current pandemic has caused chaos throughout the world. While there are few vaccines available now, there is the need for better treatment alternatives in line with preventive measures against COVID-19. Along with synthetic chemical compounds, phytochemicals cannot be overlooked as candidates for drugs against severe respiratory coronavirus 2 (SARS-CoV-2). The important role of secondary metabolites or phytochemical compounds against coronaviruses has been confirmed by studies that reported the anti-coronavirus role of glycyrrhizin from the roots of Glycyrrhiza glabra. The study demonstrated that glycyrrhizin is a very promising phytochemical against SARS-CoV, which caused an outbreak in 2002–2003. Similarly, many phytochemical compounds (apigenin, betulonic acid, reserpine, emodin, etc.) were isolated from different plants such as Isatis indigotica, Lindera aggregate, and Artemisia annua and were employed against SARS-CoV. However, owing to the geographical and seasonal variation, the quality of standard medicinal compounds isolated from plants varies. Furthermore, many of the important medicinal plants are either threatened or on the verge of endangerment because of overharvesting for medicinal purposes. Therefore, plant biotechnology provides a better alternative in the form of in vitro culture technology, including plant cell cultures, adventitious roots cultures, and organ and tissue cultures. In vitro cultures can serve as factories of secondary metabolites/phytochemicals that can be produced in bulk and of uniform quality in the fight against COVID-19, once tested. Similarly, environmental and molecular manipulation of these in vitro cultures could provide engineered drug candidates for testing against COVID-19. The in vitro culture-based phytochemicals have an additional benefit of consistency in terms of yield as well as quality. Nonetheless, as the traditional plant-based compounds might prove toxic in some cases, engineered production of promising phytochemicals can bypass this barrier. Our article focuses on reviewing the potential of the different in vitro plant cultures to produce medicinally important secondary metabolites that could ultimately be helpful in the fight against COVID-19.
Dissection of Drought Tolerance in Upland Cotton Through Morpho-Physiological and Biochemical Traits at Seedling Stage
Authors: Zahid Zobia , Khan Muhammad Kashif Riaz , Hameed Amjad , Akhtar Muhammad , Ditta Allah , Hassan Hafiz Mumtaz , Farid Ghulam
Published: March 2021

Cotton is an important fiber and cash crop. Extreme water scarceness affects the growth, quality, and productivity of cotton. Water shortage has threatened the future scenario for cotton growers, so it is imperative to devise a solution to this problem. In this research, we have tried to machinate a solution for it. 23 genotypes have been screened out against drought tolerance at the seedling stage by evaluating the morphological, physiological, and biochemical traits in a triplicate completely randomized design plot experiment with two water regimes [50 and 100% field capacity]. Genotypic differences for all the morphological and physiological traits revealed highly significant differences except transpiration rate (TR). Moreover, the interaction between genotype and water regime (G × W) was highly significant for root length (RL, 5.163), shoot length (SL, 11.751), excised leaf water loss (ELWL, 0.041), and stomatal conductance (SC, 7.406). A positively strong correlation was found in TR with relative water content (RWC; 0.510) and SC (0.584) and RWC with photosynthesis (0.452) under drought conditions. A negative correlation was found in SC with SL (−0.428) and photosynthesis (−0.446). Traits like RL, SL, SC, photosynthesis, proline, catalase, and malondialdehyde were visible indicators, which can differentiate drought-tolerant genotypes from the susceptible ones. A wide range of diversity was found in all the morpho-physiological traits with the cumulative variance of four principal components (PCs) 83.09% and three PCs 73.41% under normal and water-stressed conditions, respectively, as per the principal component analysis. Hence, selection criteria can be established on the aforementioned traits for the development of drought-tolerant cultivars. Moreover, it was found that out of 23 experimental varieties, NIAB-135, NIAB-512, and CIM-554 could be used to devise breeding strategies for improving drought tolerance in cotton.
Molecular Evolution and Organization of Ribosomal DNA in the Hawkweed Tribe Hieraciinae (Cichorieae, Asteraceae)
Authors: Fehrer Judith , Slavíková Renáta , Paštová Ladislava , Josefiová Jiřina , Mráz Patrik , Chrtek Jindřich , Bertrand Yann J. K.
Published: March 2021

Molecular evolution of ribosomal DNA can be highly dynamic. Hundreds to thousands of copies in the genome are subject to concerted evolution, which homogenizes sequence variants to different degrees. If well homogenized, sequences are suitable for phylogeny reconstruction; if not, sequence polymorphism has to be handled appropriately. Here we investigate non-coding rDNA sequences (ITS/ETS, 5S-NTS) along with the chromosomal organization of their respective loci (45S and 5S rDNA) in diploids of the Hieraciinae. The subtribe consists of genera Hieracium, Pilosella, Andryala, and Hispidella and has a complex evolutionary history characterized by ancient intergeneric hybridization, allele sharing among species, and incomplete lineage sorting. Direct or cloned Sanger sequences and phased alleles derived from Illumina genome sequencing were subjected to phylogenetic analyses. Patterns of homogenization and tree topologies based on the three regions were compared. In contrast to most other plant groups, 5S-NTS sequences were generally better homogenized than ITS and ETS sequences. A novel case of ancient intergeneric hybridization between Hispidella and Hieracium was inferred, and some further incongruences between the trees were found, suggesting independent evolution of these regions. In some species, homogenization of ITS/ETS and 5S-NTS sequences proceeded in different directions although the 5S rDNA locus always occurred on the same chromosome with one 45S rDNA locus. The ancestral rDNA organization in the Hieraciinae comprised 4 loci of 45S rDNA in terminal positions and 2 loci of 5S rDNA in interstitial positions per diploid genome. In Hieracium, some deviations from this general pattern were found (3, 6, or 7 loci of 45S rDNA; three loci of 5S rDNA). Some of these deviations concerned intraspecific variation, and most of them occurred at the tips of the tree or independently in different lineages. This indicates that the organization of rDNA loci is more dynamic than the evolution of sequences contained in them and that locus number is therefore largely unsuitable to inform about species relationships in Hieracium. No consistent differences in the degree of sequence homogenization and the number of 45S rDNA loci were found, suggesting interlocus concerted evolution.
Mechanism of Resistance to S-metolachlor in Palmer amaranth
Authors: Rangani Gulab , Noguera Matheus , Salas-Perez Reiofeli , Benedetti Lariza , Roma-Burgos Nilda
Published: March 2021

Herbicides are major tools for effective weed management. The evolution of resistance to herbicides in weedy species, especially contributed by non-target-site-based resistance (NTSR) is a worrisome issue in crop production globally. Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) is one of the extremely difficult weeds in southern US crop production. In this study, we present the level and molecular basis of resistance to the chloroacetamide herbicide, S-metolachlor, in six field-evolved A. palmeri populations that had survivors at the recommended field-dose (1.1 kg ai ha−1). These samples were collected in 2014 and 2015. The level of resistance was determined in dose-response assays. The effective dose for 50% control (ED50) of the susceptible population was 27 g ai ha−1, whereas the ED50 of the resistant populations ranged from 88 to 785 g ai ha−1. Therefore, A. palmeri resistance to S-metolachlor evolved in Arkansas as early as 2014. Metabolic-inhibitor and molecular assays indicated NTSR in these populations, mainly driven by GSTs. To understand the mechanism of resistance, selected candidate genes were analyzed in leaves and roots of survivors (with 1 × S-metolachlor). Expression analysis of the candidate genes showed that the primary site of S-metolachlor detoxification in A. palmeri is in the roots. Two GST genes, ApGSTU19 and ApGSTF8 were constitutively highly expressed in roots of all plants across all resistant populations tested. The expression of both GSTs increased further in survivors after treatment with S-metolachlor. The induction level of ApGSTF2 and ApGSTF2like by S-metolachlor differed among resistant populations. Overall, higher expression of ApGSTU19, ApGSTF8, ApGSTF2, and ApGSTF2like, which would lead to higher GST activity in roots, was strongly associated with the resistant phenotype. Phylogenetic relationship and analysis of substrate binding site of candidate genes suggested functional similarities with known metolachlor-detoxifying GSTs, effecting metabolic resistance to S-metolachlor in A. palmeri. Resistance is achieved by elevated baseline expression of these genes and further induction by S-metolachlor in resistant plants.
Which Task Characteristics Do Students Rely on When They Evaluate Their Abilities to Solve Linear Function Tasks? – A Task-Specific Assessment of Self-Efficacy
Authors: Siefer Katharina , Leuders Timo , Obersteiner Andreas
Published: March 2021

Self-efficacy is an important predictor of learning and achievement. By definition, self-efficacy requires a task-specific assessment, in which students are asked to evaluate whether they can solve concrete tasks. An underlying assumption in previous research into such assessments was that self-efficacy is a one-dimensional construct. However, empirical evidence for this assumption is lacking, and research on students’ performance suggests that it depends on various task characteristics (e.g., the representational format). The present study explores the potential multi-dimensionality of self-efficacy in the topic of linear functions. More specifically, we investigate how three task characteristics – (1) the representational format, (2) embedding in a real-life context, or (3) the required operation – are related to students’ self-efficacy. We asked 8th and 9th graders (N = 376) to evaluate their self-efficacy on specific linear function tasks which systematically varied along the three dimensions of task characteristics. Using confirmatory factor analysis, we found that a two-dimensional model which includes the task characteristic of real-life context (i.e., with vs. without a real-life context) fitted the data better than other two-dimensional models or a one-dimensional model. These results suggest that self-efficacy with linear functions is empirically separable with respect to tasks with vs. without a real-life context. This means that in their self-evaluation of linear function tasks students particularly rely on whether or not the linear function task is embedded in a real-life context. This study highlights the fact that even within a specific content domain students’ self-efficacy can be considered a multi-dimensional construct.
Context Breeds False Memories for Indeterminate Sentences
Authors: Riven Levi , de Almeida Roberto G.
Published: March 2021

What are the roles of semantic and pragmatic processes in the interpretation of sentences in context? And how do we attain such interpretations when sentences are deemed indeterminate? Consider a sentence such as “Lisa began the book” which does not overtly express the activity that Lisa began doing with the book. Although it is believed that individuals compute a specified event to enrich the sentential representation – yielding, e.g., “began [reading] the book” – there is no evidence that a default event meaning is attained. Moreover, if indeterminate sentences are enriched, it is not clear where the information required to generate enriched interpretations come from. Experiment 1 showed that, in isolation, there is no default interpretation for indeterminate sentences. The experiment also showed that biasing contexts constrain event interpretations and improve plausibility judgments, suggesting that event representations for indeterminate sentences are generated by context. In Experiment 2, participants heard biasing discourse contexts and later falsely recognized foil sentences containing the biased events (“Lisa began reading the book”) at the same proportion and with the same confidence as the original indeterminate sentence (“Lisa began the book”). We suggest that indeterminate sentences trigger event-enriching inferences but only in sufficiently constraining contexts. We also suggest that indeterminate sentences create two memory traces, one for the proposition consistent with the denotational, compositional meaning, and another for the proposition that is enriched pragmatically over time.
Effects of Facilitation vs. Exhibit Labels on Caregiver-Child Interactions at a Museum Exhibit
Authors: Letourneau Susan M. , Meisner Robin , Sobel David M.
Published: March 2021

In museum settings, caregivers support children's learning as they explore and interact with exhibits. Museums have developed exhibit design and facilitation strategies for promoting families' exploration and inquiry, but these strategies have rarely been contrasted. The goal of the current study was to investigate how prompts offered through staff facilitation vs. labels printed on exhibit components affected how family groups explored a circuit blocks exhibit, particularly whether children set and worked toward their own goals, and how caregivers were involved in children's play. We compared whether children, their caregivers, or both set goals as they played together, and the actions they each took to connect the circuits. We found little difference in how families set goals between the two conditions, but did find significant differences in caregivers' actions, with caregivers in the facilitation condition making fewer actions to connect circuits while using the exhibit, compared to caregivers in the exhibit labels condition. The findings suggest that facilitated and written prompts shape the quality of caregiver-child interactions in distinct ways.
Staying Ahead of the Epidemiologic Curve: Evaluation of the British Columbia Asthma Prediction System (BCAPS) During the Unprecedented 2018 Wildfire Season
Authors: Henderson Sarah B. , Morrison Kathryn T. , McLean Kathleen E. , Ding Yue , Yao Jiayun , Shaddick Gavin , Buckeridge David L.
Published: March 2021

Background: The modular British Columbia Asthma Prediction System (BCAPS) is designed to reduce information burden during wildfire smoke events by automatically gathering, integrating, generating, and visualizing data for public health users. The BCAPS framework comprises five flexible and geographically scalable modules: (1) historic data on fine particulate matter (PM2.5) concentrations; (2) historic data on relevant health indicator counts; (3) PM2.5 forecasts for the upcoming days; (4) a health forecasting model that uses the relationship between (1) and (2) to predict the impacts of (3); and (5) a reporting mechanism.

Methods: The 2018 wildfire season was the most extreme in British Columbia history. Every morning BCAPS generated forecasts of salbutamol sulfate (e.g., Ventolin) inhaler dispensations for the upcoming days in 16 Health Service Delivery Areas (HSDAs) using random forest machine learning. These forecasts were compared with observations over a 63-day study period using different methods including the index of agreement (IOA), which ranges from 0 (no agreement) to 1 (perfect agreement). Some observations were compared with the same period in the milder wildfire season of 2016 for context.

Results: The mean province-wide population-weighted PM2.5 concentration over the study period was 22.0 μg/m3, compared with 4.2 μg/m3 during the milder wildfire season of 2016. The PM2.5 forecasts underpredicted the severe smoke impacts, but the IOA was relatively strong with a population-weighted average of 0.85, ranging from 0.65 to 0.95 among the HSDAs. Inhaler dispensations increased by 30% over 2016 values. Forecasted dispensations were within 20% of the observed value in 71% of cases, and the IOA was strong with a population-weighted average of 0.95, ranging from 0.92 to 0.98. All measures of agreement were correlated with HSDA population, where BCAPS performance was better in the larger populations with more moderate smoke impacts. The accuracy of the health forecasts was partially dependent on the accuracy of the PM2.5 forecasts, but they were robust to over- and underpredictions of PM2.5 exposure.

Conclusions: Daily reports from the BCAPS framework provided timely and reasonable insight into the population health impacts of predicted smoke exposures, though more work is necessary to improve the PM2.5 and health indicator forecasts.
Comparison of Retrospective and Prospective Falls Reporting Among Community-Dwelling Older People: Findings From Two Cohort Studies
Authors: Romli Muhammad Hibatullah , Mackenzie Lynette , Tan Pey June , Chiew Re On , Tan Shun Herng , Tan Maw Pin
Published: March 2021

Background:While prospective recording is considered as the gold standard, retrospective recall is widely utilized for falls outcomes due to its convenience. This brings about the concern on the validity of falls reporting in Southeast Asian countries, as the reliability of falls recall has not previously been studied. This study aimed to evaluate the reliability of retrospective falls recall compared to prospective falls recording.

Methods:A secondary analysis of data from two prospective recording methods, falls diary and falls calendar, from two different research projects were obtained and analyzed. Retrospective falls recall was collected either through phone interview or follow-up clinic by asking the participants if they had fallen in the past 12 months.

Results:Two-hundred-sixty-eight and 280 elderly participated in the diary and calendar groups, respectively. Moderate (46%) and poor (11%) return rates were found on completed diary and calendar recording. Under-(32%) and overreporting (24%) of falls were found in diary compared to only 4% of overreporting for the calendar. Retrospective recall method achieved 57% response rate for the diary group (followed up at clinic) and 89% for the calendar group (followed up via telephone interview). Agreement between retrospective and prospective reporting was moderate for the diary (kappa =0.44;p< 0.001) and strong for the calendar (kappa = 0.89;p< 0.001).

Conclusion:Retrospective recall is reliable and acceptable in an observation study within healthy community older adults, while the combination of retrospective and prospective falls recording is the best for an intervention study with frailer older population. Telephone interview is convenient, low cost, and yielded a high response rate.
Women Face to Fear and Safety Devices During the COVID-19 Pandemic in Italy: Impact of Physical Distancing on Individual Responsibility, Intimate, and Social Relationship
Authors: Parisi Rosa , Lagomarsino Francesca , Rania Nadia , Coppola Ilaria
Published: March 2021

The COVID-19 pandemic of 2020 in Italy had its first epidemic manifestations on January 31, 2020. The socio-sanitary rules imposed by the government concerned the social distance and management of intimate relationships, the sense of individual responsibility toward public health. Physical distancing and housing isolation have produced new representations of intrafamily, generational, neighborhood, community responsibility, bringing out a new “medicalized dimension” of society. In light of this contextual framework, the research aims are to analyze how: the perception of individual responsibility for public and familial health and physical distancing has redrawn the relation between subjects-family-community; the State's technical-health intervention has reformulated the idea of social closeness, but also how the pandemic fear and social confinement has re-evaluated a desire for community, neighborhood, proximity; during the lockdown families, friends, neighbors have reconstructed feelings of closeness and forms of belonging. The methodology used is quanti-qualitative and involved 300 women through an online questionnaire. The data collected highlight how the house during the lockdown is perceived as a safe place and how women implement both the recommendations and the behaviors aimed at preventing contagion, but also ways that allow coping with the situation from a perspective of well-being. Furthermore, the data show how the dimension of distancing has loosened the relational dimension outside the family unit, with a greater distancing compared to pre-pandemic data. However, the majority of women report that they have joined solidarity initiatives, demonstrating that they want to maintain ties and participate actively in community life.
Do Drug Accessibility and OOP Burden Affect Health-Related Quality of Life of Patients With Chronic Diseases? — EQ-5D-5L Evaluation Evidence From Five Districts in China
Authors: Tang Shaoliang , Gong Ying , Liu Meixian , Yang Duoer , Tang Kean
Published: March 2021

Purpose: The dependence of patients with chronic diseases on drugs may affect their health-related quality of life (HRQoL). This study aims to assess the relationship between the direct economic burden caused by out-of-pocket (OOP) payments, drug accessibility, sociodemographic characteristics, and health-related quality of life.

Methods: 1,055 patients with chronic diseases from Gansu, Hebei, Sichuan, Zhejiang, and Tianjin were investigated. Data collection included basic conditions and economic and health insurance conditions of patients with chronic diseases. The CLAD and Tobit regression models were used to analyze and compare the health-related quality of life and influencing factors of patients with chronic diseases in five districts. Differentiated analysis was conducted through sub-sample regression to explore the variable health effects of patients with single and multiple diseases.

Results: A total of 1,055 patients with chronic diseases participated in the study, 54.4% of whom were women. The overall average utility score was 0.727, of which Sichuan Province was the highest with 0.751. Participants reported the highest proportion of pain/discomfort problems, while patients reported the least problems with self-care. The improvement of drug accessibility and the reduction of the burden of out-of-pocket expenses have significant positive effects on HRQoL. Various sociodemographic factors such as age and gender also have significant impact on HRQoL of patients with chronic diseases. HRQoL of patients with multiple chronic diseases is more affected by various influencing factors than that of patients with single disease.

Conclusion: In order to improve the quality of life of patients with chronic diseases, it is of great importance to ensure the accessibility of drugs and reduce patients' medication burden. Future focus should shift from preventing and controlling chronic diseases as individual diseases to meeting the comprehensive health needs of people suffering from multiple diseases.
A Novel Program to Improve Cognitive Function in Individuals With Dementia Using Transcranial Alternating Current Stimulation (tACS) and Tutored Cognitive Exercises
Authors: Moussavi Zahra , Kimura Kazushige , Kehler Lonnie , de Oliveira Francisco Cristina , Lithgow Brian
Published: March 2021

The effects of cognitive exercises on the healthy aging population is controversial. Transcranial alternating current stimulation (tACS) is considered a promising tool for modulating brain oscillation. Research is lacking on its long-lasting cognitive/therapeutic effect. This is the first pilot study to explore the effect of a regimen of cognitive exercises with and without tACS on older adults with dementia. The study groups were 28 individuals (age 56–83 years) enrolled into two groups: Exr Group, who received cognitive exercises only and the Exr + tACS Group who received tACS at 40 Hz simultaneously with cognitive exercises for a period of 4 consecutive weeks, 5 days/week, two 30 min-sessions/day; all the training sessions were tutored. The cognitive exercises were applied using the MindTriggers app. They were assessed at pre and post intervention and also one month after the end of trial (follow-up) with an independent assessment (WMS-IV) as the primary outcome measure. The results show significant cognitive improvement at post-intervention in both groups, while the Exr + tACS protocol lead to superior cognitive improvement at follow-up session. The most important outcomes of this study are: 1) The tutored repeated practice of the MindTriggers app exercises does significantly improve the cognitive functions of older adults with dementia and that that improvement lasts for at least one month after the end of the intervention, and 2) The application of tACS increases the positive effects of cognitive exercises with the positive effect lasting an even longer period of time than exercises alone; in other words we speculate that it may lead to long-term potentiation.
Modeling and Reconstruction of State Variables for Low-Level Control of Soft Pneumatic Actuators
Authors: Ibrahim Serhat , Krause Jan Christoph , Olbrich Alexander , Raatz Annika
Published: March 2021

To further advance closed-loop control for soft robotics, suitable sensor and modeling strategies have to be investigated. Although there are many flexible and soft sensors available, the integration into the actuator and the use in a control loop is still challenging. Therefore, a state-space model for closed-loop low-level control of a fiber-reinforced actuator using pressure and orientation measurement is investigated. To do so, the integration of an inertial measurement unit and geometric modeling of actuator is presented. The piecewise constant curvature approach is used to describe the actuator’s shape and deformation variables. For low-level control, the chamber’s lengths are reconstructed from bending angles with a geometrical model and the identified material characteristics. For parameter identification and model validation, data from a camera tracking system is analyzed. Then, a closed-loop control of pressure and chambers’ length of the actuator is investigated. It will be shown, that the reconstruction model is suitable for estimating the state variables of the actuator. In addition, the use of the inertial measurement unit will demonstrate a cost-effective and compact sensor for soft pneumatic actuators.
A Novel Anastomosis Technique for Laparoscopic Pancreaticoduodenectomy: Case Series of Our Center's Experience
Authors: Sun Pi-Jiang , Yu Yan-Hua , Li Jian-Wei , Cui Xi-Jun
Published: March 2021

Background: Laparoscopic pancreaticoduodenectomy has developed rapidly in recent years. Postoperative pancreatic fistula is still the most dangerous complication of laparoscopic pancreaticoduodenectomy. Baumgart pancreaticojejunostomy is considered one of the safest anastomosis procedures, with low rates of pancreatic fistula. We modified Blumgart pancreaticojejunostomy and applied the modified procedure during laparoscopic pancreaticoduodenectomy. The modified procedure entailed a longitudinal U-shaped suture through the pancreas for anastomosis of the pancreatic duct and the jejunal mucosa.

Methods: We prospectively collected and retrospectively analyzed the data of 120 patients who underwent laparoscopic pancreaticoduodenectomy from January 2016. The total operative time, time for complete pancreaticojejunostomy, postoperative pancreatic fistula rate, postoperative delayed gastric emptying, postoperative bleeding, postoperative length of hospital stays, and mortality within 90 days after surgery were analyzed. An analysis of laparoscopic pancreaticojejunostomy compared with open pancreaticojejunostomy is also reported.

Results: In the laparoscopic pancreaticojejunostomy group, the average total operative time, the average time for complete pancreaticojejunostomy, and the average intraoperative blood loss were 271 min, 35.3 min, and 184 ml, respectively. The total postoperative clinically relevant pancreatic fistula rate was 9.2% (Grade B and C fistulas). The incidence rates of postoperative delayed gastric emptying and postoperative biliary fistula were ~2.5 and 1.7%, respectively. The postoperative bleeding rate was 0.83%, and the average postoperative indwelling time of the abdominal drainage tube was 7.3 days. The postoperative length of hospital stay was 10.8 days, and the mortality rate within 90 days after surgery was 0.83%. The rates of clinically relevant postoperative clinically relevant pancreatic fistula are comparable between laparoscopic and open surgery, there were no other severe postoperative complications in either group. The mean postoperative length of hospital stay was significantly shorter in the laparoscopic pancreaticojejunostomy group.

Conclusion: The modified laparoscopic-adapted Blumgart anastomosis simplifies and facilitates the creation of the pancreaticojejunostomy in laparoscopic pancreaticoduodenectomy. The rates of clinically relevant postoperative pancreatic fistula are comparable with those obtained by open surgery, and length of stay are shoter.
Diversity and Toxigenicity of Mycobiota in Grain Corn: A Case Study at Pioneer Grain Corn Plantations in Terengganu, Malaysia
Authors: Yazid Siti Nur Ezzati , Ng Wan Jing , Selamat Jinap , Ismail Siti Izera , Samsudin Nik Iskandar Putra
Published: March 2021

Malaysia has begun to locally mass-cultivate grain corn to reduce import dependency for animal feed industries. Since the Malaysian tropical climate constantly exposes grain corn to fungal colonization and mycotoxin production by mycotoxigenic species, it is, therefore, important to investigate the presence of fungal species, especially the mycotoxigenic strains in the Malaysian grain corn agroecosystem. In the present work, corn kernel, tassel, plant debris, and soil were collected from two pioneer grain corn farms (Kampong Dadong, KD; Rhu Tapai, RT), and morphological and molecular identifications were conducted. A total of 131 fungal isolates from 30 fungal species were recovered. Both KD and RT yielded log 4.7–6.7 CFU/g total fungal loads. Fusarium verticillioides was predominant in both farms, followed by the phytopathogenic Lasiodiplodia theobromae and the mycotoxigenic Aspergillus flavus, A. niger, F. incarnatum, and F. proliferatum. Mycotoxin analyses by high-performance liquid chromatography revealed that among 30 mycotoxigenic isolates tested for aflatoxins, deoxynivalenol, fumonisins, HT-2, T-2, ochratoxins A, and zearalenone, approximately 25 of the isolates could produce at least one mycotoxin in vitro. The present work serves as a baseline for more comprehensive research to better predict and control fungal contamination and the subsequent mycotoxin accumulation in Malaysian grain corn agroecosystems.
The Deviation between Dairy Cow Metabolizable Energy Requirements and Pasture Supply on a Dairy Farm Using Proximal Hyperspectral Sensing
Authors: Duranovich Federico , López-Villalobos Nicolás , Shadbolt Nicola , Draganova Ina , Yule Ian , Morris Stephen
Published: March 2021

This study aimed at determining the extent to which the deviation of daily total metabolizable energy (MEt) requirements of individual cows from the metabolizable energy (ME) supplied per cow (DME) varied throughout the production season in a pasture-based dairy farm using proximal hyperspectral sensing (PHS). Herd tests, milk production, herbage and feed allocation data were collected during the 2016–2017 and 2017–2018 production seasons at Dairy 1, Massey University, New Zealand. Herbage ME was determined from canopy reflectance acquired using PHS. Orthogonal polynomials were used to model lactation curves for yields of milk, fat, protein and live weights of cows. Daily dietary ME supplied per cow to the herd and ME requirements of cows were calculated using the Agricultural Food and Research Council (AFRC) energy system of 1993. A linear model including the random effects of breed and cow was used to estimate variance components for DME. Daily herd MEt estimated requirements oscillated between a fifth above or below the ME supplied throughout the production seasons. DME was mostly explained by observations made within a cow rather than between cows or breeds. Having daily estimates of individual cow requirements for MEt in addition to ME dietary supply can potentially contribute to achieving a more precise fit between supply and demand for feed in a pasture-based dairy farm by devising feeding strategies aimed at reducing DME.
The Nexus between Rural Household Livelihoods and Agricultural Functions: Evidence from China
Authors: Liu Bencheng , Fang Yangang
Published: March 2021

Understanding the relationship between households’ livelihoods and agricultural functions is important for regulating and balancing households’ and macrosocieties’ agricultural functional needs and formulating better agricultural policies and rural revitalization strategies. This paper uses peasant household survey data obtained from the China Family Panel Studies (CFPS) and statistical analysis methods, to analyze the differences in livelihood assets and agricultural functions of households with different livelihood strategies and the relationship between livelihood assets and agricultural functions. Households are categorized based on their livelihood strategies as full-time farming households, part-time farming I households, part-time farming II households, and non-farming households. The agricultural product supply and negative effects of the ecological service function of full-time farming households are higher than those of part-time farming and non-farming households. Part-time farming I households have the strongest social security function, while non-farming households have the weakest social security function. Non-farming households have the strongest leisure and cultural function, while part-time farming I households have the weakest leisure and cultural function. Households’ demand for agricultural functions is affected by livelihood assets. Effective measures should be taken to address contradictions in the agricultural functional demands of households and macrosocieties.
Construction and Experimental Verification of Sloped Terrain Soil Pressure-Sinkage Model
Authors: Pan Guanting , Sun Jingbin , Wang Xiaole , Yang Fuzeng , Liu Zhijie
Published: March 2021

The construction of a scientific and effective soil pressure-sinkage model under sloped terrain condition has important guiding significance for the investigation of the soil compaction effect. It is also important for the theoretical calculation of driving resistance and design optimization of the undercarriage structure of hillside metal-tracked tractors (HMTs). The classic Bekker’s pressure-sinkage model does not consider the influence of the soil water content, bulk density, slope angle, and other factors; therefore, it cannot be directly used to investigate the relationship between the soil compaction and its sinkage under sloped terrain conditions. To solve this problem, this study first verified that the soil water content and bulk density exert significant effects on the pressure–sinkage relationship under flat terrain condition. Secondly, a pressure-sinkage test was carried out using the quadratic rotation orthogonal combination design method, and the soil water content, density, and slope angle were considered. The pressure-sinkage curves of sloped terrain soils from Yangling and Yangxian in Shaanxi Province, and Huining and Jingning in Gansu Province were obtained. Then the pressure–sinkage parameters (sinkage exponent, cohesive modulus, and frictional modulus) were calculated using the weighted least-squares method. Thirdly, the mathematical relationship between the parameters and the soil water content, bulk density, and slope angle was obtained. Then Bekker’s model was modified to obtain the pressure–sinkage model of sloped terrain. Finally, the control variable method under slope angle of 10°, soil water content of 10%, and bulk density of 2 mg·m−3 were used to validate the model. The results revealed that the root-mean-square error between the calculated pressure value of the model and the measured value of the film pressure sensor was 1.614, 1.601, and 0.822, respectively. In the dynamic operation of a hillside tractor prototype, the calculated pressures between the supporting wheels were close to the measured values. It indicates that the modified soil pressure–sinkage model is more suitable for calculating the force at the bottom of the track between the supporting wheels. It can also provide an important theoretical basis for accurately calculating the pressure–sinkage parameters of sloped terrain soil. Additionally, this approach could provide theoretical and technical support for the rational arrangement of HMT undercarriages to reduce the soil sinkage and driving resistance.
Comprehensive Analysis of the Histone Deacetylase Gene Family in Chinese Cabbage (Brassica rapa): From Evolution and Expression Pattern to Functional Analysis of BraHDA3
Authors: Eom Seung Hee , Hyun Tae Kyung
Published: March 2021

Histone deacetylases (HDACs) are known as erasers that remove acetyl groups from lysine residues in histones. Although plant HDACs play essential roles in physiological processes, including various stress responses, our knowledge concerning HDAC gene families and their evolutionary relationship remains limited. In Brassica rapa genome, we identified 20 HDAC genes, which are divided into three major groups: RPD3/HDA1, HD2, and SIR2 families. In addition, seven pairs of segmental duplicated paralogs and one pair of tandem duplicated paralogs were identified in the B. rapa HDAC (BraHDAC) family, indicating that segmental duplication is predominant for the expansion of the BraHDAC genes. The expression patterns of paralogous gene pairs suggest a divergence in the function of BraHDACs under various stress conditions. Furthermore, we suggested that BraHDA3 (homologous of Arabidopsis HDA14) encodes the functional HDAC enzyme, which can be inhibited by Class I/II HDAC inhibitor SAHA. As a first step toward understanding the epigenetic responses to environmental stresses in Chinese cabbage, our results provide a solid foundation for functional analysis of the BraHDAC family.
Seasonal Dynamics of Soil Moisture in an Integrated-Crop-Livestock-Forestry System in Central-West Brazil
Authors: Glatzle Sarah , Stuerz Sabine , Giese Marcus , Pereira Mariana , de Almeida Roberto Giolo , Bungenstab Davi José , Macedo Manuel Claudio M. , Asch Folkard
Published: March 2021

Integrated-crop-livestock-forestry (ICLF) systems are currently promoted as a measure for sustainable intensification of agricultural production. However, due to complex interactions among ICLF components, we are still lacking evidence about the system’s resilience regarding water availability, especially for regions characterized by pronounced wet and dry seasons and frequent droughts. For a mature ICLF system in the Cerrado biome of central-west Brazil comprising rows of eucalyptus trees (Eucalyptus grandis x Eucalyptus urophylla, H13 clone) at a spacing of 22 m in combination with Brachiaria brizantha cv. BRS Piatã pasture we continuously measured soil moisture (SM) until 1 m depth and supported this data with measurements of photosynthetically active radiation (PAR) and aboveground green grass biomass (AGBM) across transects between the tree rows for almost two years. Across the seasons a distinct gradient was observed with SM being lower close to the tree rows than in the space between them. During winter SM decreased to critical values near the tree lines in the topsoil. During spring and summer, incident PAR was 72% and 86% lower close to the trees than at the center point. For autumn and winter PAR was more evenly distributed between the tree rows due to inclination with notably up to four times more radiation input near the tree lines compared to spring and summer. AGBM showed a clear distribution with maximum values in the center and about half of the biomass close to the tree rows. Our data suggest that, restrictions in AGBM accumulation shifted among seasons between water limitations in winter and light limitations during summer. Interestingly, SM changes during wetting and drying events were most pronounced in subsoils near the tree rows, while the topsoil showed much less fluctuations. The subsoil in central position showed the lowest SM dynamics in response to drought maintaining a relative high and constant SM content, therefore functioning as important water reservoirs likely improving the resilience of the system to drought stress. Results of this study could help to improve management and the design of ICLF systems in view of sustainability and resistance to (water) crises but should be further supported by in depth analysis of soil water dynamics as affected by climate gradients, soil types and different management practices.
Sustainable Transfer of Tomato Landraces to Modern Cropping Systems: The Effects of Environmental Conditions and Management Practices on Long-Shelf-Life Tomatoes
Authors: Casals Joan , Martí Montserrat , Rull Aurora , Pons Clara
Published: March 2021

The individual effects of biotic and abiotic factors on tomatoes have been widely reported. However, under commercial conditions, multiple interactions between factors occur, masking or even changing the direction of their effects in some cases. Here we report a comprehensive analysis of preharvest factors affecting yield, quality (soluble solids content, fruit color, and firmness), and shelf-life of long-shelf-life Mediterranean varieties of tomatoes. We studied five long-shelf-life genotypes under 16 growing environments, including tunnel and open-air systems and suboptimal to excessive fertigation (22–142% crop evapotranspiration). The results enabled us to classify traits into three groups according to the importance of the contributions of different types of factors: mainly genotype (ripening earliness and firmness), genotype plus environment (yield, fruit weight, water-use efficiency (WUE)), or genotype plus environment plus the interaction between genotype and environment (cracking, soluble solids content, and shelf-life). Under similar management practices, open-air conditions optimized yields, and high fertigation doses improved yield and marketability (firmness), but reduced quality (redness and soluble solids content). WUE was maximized under low-input cropping systems (comparable to traditional agrosystems), and the balance between WUE and yield was optimized when fertigation was adjusted to the requirements of the crop. Shelf-life was negatively correlated with high-yielding environments, and day–night amplitude in relative humidity was strongly correlated with the incidence of fruit cracking. The present study sheds light on the contributions of environment and management practices on tomato yield and quality, and provides a basis on which to select better management practices for the novel commercial group of European long-shelf-life tomato landraces.
Sweet Corn Research around the World 2015–2020
Authors: Revilla Pedro , Anibas Calli M. , Tracy William F.
Published: March 2021

Modern sweet corn is distinguished from other vegetable corns by the presence of one or more recessive alleles within the maize endosperm starch synthesis pathway. This results in reduced starch content and increased sugar concentration when consumed fresh. Fresh sweet corn originated in the USA and has since been introduced in countries around the World with increasing popularity as a favored vegetable choice. Several reviews have been published recently on endosperm genetics, breeding, and physiology that focus on the basic biology and uses in the US. However, new questions concerning sustainability, environmental care, and climate change, along with the introduction of sweet corn in other countries have produced a variety of new uses and research activities. This review is a summary of the sweet corn research published during the five years preceding 2021.