bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of suppression of internal microflora by tetracycline on the level of manifestation of the hybrid dysgenesis of Drosophila melanogaster
Authors: Gorodnyanski I. D. , Vorobyova L. I.
Published: March 2020

Aim. The main goal of this article was to assess changes in the frequency of the development of hybrid dysgenesis in the Oregon-R line of Drosophila melanogaster, observed under the influence of tetracycline. Methods. The line of Drosophila melanogaster Oregon R (Cambridge), in the genome of which there are full-sized and functionally active copies of the MGE hobo, was investigated. Also, this line is infected with wolbachia of the wMel strain. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. This article presents the results of studying the effect of suppression of the internal microflora of Drosophila melanogaster – in particular, the parasitic bacterium Wolbachia pipientis - on the level of manifestation of hybrid dysgenesis. After exposure to tetracycline, a significant increase in the proportion of dysgenic individuals within the line was observed. Conclusions. The results obtained may be associated with a violation of the system of specific interactions of the host organism and internal microflora.

Keywords: mobile genetic elements, hybrid dysgenesis, Drosophila melanogaster, Wolbachia pipientis.
Indexes of natural selection in Kharkiv population
Authors: Kozak N. O. , Atramentova L. A.
Published: March 2020

Aim. Reproductive behavior of women has particular importance in the reproduction of the population and depends on cultural characteristics and religious views. Number of birth and medical abortions become artificially regulated; nevertheless selection in human populations does not disappear. Therefore, the aim of this task to study the direction and intensity of indexes of natural selection and the dynamics of the gene pool in the Kharkiv population for two adjacent generations. Methods. The material was collected in 2017. Anonymous questionnaires were conducted for women in postreproductive age. The questionnaire includes questions which reflect a woman's gynecological and obstetrical anamnesis. The number of pregnancies and their outcomes was calculated: medical and spontaneous abortions, ectopic pregnancies, number of stillbirths and live births. Results. In one generation, the total selection index decrease from 0.29 to 0.28, while the differential fertility index is much higher than the differential mortality index. The average number of pregnancies and medical abortions reduced in the younger generation with statistically significant difference. Conclusions. The Kharkov population is characterized by a narrow type of reproduction. The dynamics of Crow's indexes are in line with global trends. From the older generation to the younger the total selection index decreases gradually.Keywords: reproductive characteristics,Kharkov population, Crow's index, selection, generations.
Protein polimorphism of cross components at development of common wheat lines with introgression from Triticum migushovae Zhir.
Authors: Pasichnyk T. V. , Antonyuk M. Z. , Ternovska T. K.
Published: March 2020

Aim. Determine presence/absence of polymorphism at genes coding wheat proteins with known chromosome localization in order to determine cross components for development of introgressive lines T. aestivum/T. miguschovae, which are optimal for screening progeny for studied proteins. Methods. Protein electrophoresis in PAAG, visualization and comparison of spectra. Results. Electrophoretic spectra components which could be used as markers of chromosomes of Ab, G, D genomes of Migushova wheat, and A, B and D genomes of four cultivars of common wheat were identified for 1-st (gliadins, glutenins), 3-rd (leaf and seed esterase, peroxidase), 4-th (beta-amylase, acid phosphatase), 6-th (gliadins, alfa-amylase), 7-th (alfa-amylase) groups of homeological chromosomes. Conclusions. Progeny from any of the four common wheat cultivars can be studied for the presence of Migushova wheat chromosomes that substituted common wheat chromosomes of 1-st, 3-rd, 4-th, 6-th, and 7-th homeological groups, however, effectiveness of studied protein markers varied for different cultivars.Keywords: wheat introgression, Fusarium head blight, Triticum miguschovae, storage proteins, isoenzymes.
The lifespan of starving flies of different Drosophila melanogaster stocks: effects of caffeine and He-Ne laser light
Authors: Gorenskaya O. V. , Filiponenko N. S. , Shckorbatov Yu. G.
Published: March 2020

Aim. Analysis of the chronic effects of caffeine and laser radiation on starvation resistance in Drosophila melanogaster, in depends on the genotype. Methods. The experiments were carried out in wild type stocks of Drosophila melanogaster: Canton-S and Oregon; mutant stock ebony and stocks with mutation ebony saturated by stocks Canton-S and Oregon – ebonyC-S and ebonyOr. Caffeine was applied in concentration of 0.5 mg/ml in the cultural medium. Flies were exposed to He-Ne laser light of wavelength 632.8 nm and surface power density 0.03 mW/cm2 for 5 minutes. Control flies were grown under standard living conditions. Results. The lifespan during starvation dependence on a genotype (17.62 % and 19.51 %), external factors (h = 17.02 % and 19.64 %) and on the combined factors – h = 4.37 % and 2.42 % (for males and males, respectively) was shown. Conclusions. Almost in all experimental variants caffeine and laser light induced the extension lifespan during starvation. The simultaneous applying of both factors (caffeine+laser light) induced the maximal lifespan extension in Drosophila stocks C-S, ebony, and ebonyOr. Such additive effect was not observed in ebonyC-S flies. We connect the observed effects of caffeine and laser light with hormesis effect.Keywords: drosophila, genotype, lifespan during starvation, mutation, stress tolerance.
Analysis of cellular localization of РН domain of Bcr-Abl with USP1 protein and development of software for estimation of their phosphorylation sites
Authors: Antonenko S. V. , Gurianov D. S. , Kravchuk I. V. , Telegeev G. D.
Published: March 2020

Aim. This work was dedicated to investigation of cellular localization РН domain of Bcr-Abl with USP1 protein and to develop useful tool to aggregate information about protein phosphorylation sites. Methods. Mammalian cell transfection. Primers design. Widefield and STED fluorescent microscopy. Basic software development in Python. Results. Co-localization between USP1 and PH domain of Bcr-Abl has been detected. Primers for main domains of USP1 have been designed. Integration tool phospho-aggregate has been developed. Super-resolution image of intracellular distribution of PH domain of Bcr was obtained. Conclusions. Nuclear localization of USP1 in 293T cells and also point co-localizations of USP1 and РН domain of Bcr-Abl oncoloprotein show strong association of investigated proteins with specific cellular structures. Identification of these cellular structures can help to find out biological functions of of USP1 in CML. Tool phospho-aggregate can be used to simplify analysis and comparison of protein phosphorylation sites. Subdiffraction imaging of intracellular distribution of PH domain of Bcr shows that it clusters in structures that resemble shape of clathrin-coated vesicles.Keywords: Bcr-Abl, USP1, PH domain, cortactin, cellular localization, phosphorylation sites.
Polymorphism in the sequence of Glu1 gene in populations of Thinopyrum intermedium as a possible adaptive trait
Authors: Antonyuk M. Z. , Iefimenko T. S. , Ternovska T. K.
Published: March 2020

Aim. Compare structures of Glu genes, extracted from Th. intermedium plants grown in different microgeographic conditions. Methods. PCR with DNA samples of Th. intermedium and primers to Glu-1Dx and Glu-1Dy gene regions, sequencing and comparative analysis of polymorphic amplicons and sequences from databases. Results. Central fragments of Glu-1Dx and Glu-1Dy from plants grown in different microgeographic conditions had significant differences compared to corresponding sequences of Aegilops tauschii (Glu-1Dx), Thinopyrum intermedium (Glu-1St*2x), Triticum aestivum (Glu-1Dy) obtained from databases. Both genes had point mutations, deletions and insertions, which transformed glutamine coding triplets characteristic for storage proteins to stop-codons, also codons for cysteine, methionine and proline appeared. These aminoacids affect protein surface charge, hydrophilic/hydrophobic properties, conformation density, and structure stability. Conclusions. Changes in high molecular weight glutenin structure, which were caused by the observed mutations, could affect protease ability to effectively hydrolases them, which consequently could affect seed germination rate. Slow seed germination might be one of the mechanisms for survival of wild cereal species Thinopyrum intermedium under unfavorable environmental conditions.Keywords: Thinopyrum intermedium, Glu-1Dх, Glu-1Dy, sequence, point mutations, stop codon.
Intravarietal intron-length polymorphism of β-tubulin genes in belorussian landraces of Linum usitatissimum L.
Authors: Rabokon A. M. , Pirko Ya. B. , Kalafat L. O. , Guzenko Ye. V. , Bogdanova M. V. , Sakovich V. I. , Lemesh V. A. , Blume Ya. B.
Published: March 2020

Aim. The aim of the work was to evaluate the possibilities of using the β-tubulin intron length (TBP, Tubulin Based Polymorphism) for genetic differentiation of ancient flax varieties (landraces), plants that was historically formed in Belarus. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments (β-tubulin introns) were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The size of the amplified fragments varied from 400 bp to 1900 bp. It was found that 25 of 30 studied varieties (landraces) were genetically heterogeneous. The total number of allele phenotypes was 7, and the value of PIC (Polymorphism Information Content) varied from 0.0 to 0.72. Conclusions. The data obtained make it possible to recommend landraces as a source of genes for increasing the genetic diversity of the existing flax gene pool, and the TBP method can be applied both in molecular-phylogenetic analysis and in molecular selection of flax.

Keywords: Linum usitatissimum, landrace, molecular markers, DNA fingerprinting, β-tubulin genes, intron polymorphism.
Adaptation of corn plants to chronic ultraviolet irradiation
Authors: Zhuk V. V. , Mikhieiev A. N. , Ovsyannikova L. G.
Published: March 2020

Aim. The adaptation of young corn plants (Zea mays L.) to the effect of chronic ultraviolet B (UV-B) irradiation during vegetative growth and formation of photosynthetic apparatus of leaves was studied. Methods. Corn plants hybrid Dostatok 300 MB in the phase of two developed leaves was irradiated with UV-B in dose of 2 kJ/m2 per day with a power of 1 W/m2 in long day conditions during 12 days. During this period, the growth of plants was measured, content of photosynthetic pigments and endogenous hydrogen peroxide (HP) was determined in the leaves. Results. It was established that the effect of chronic UV‑B irradiation caused the temporary inhibition of leaf growth, synthesis of photosynthetic pigments and increasing of HP content in them. Then the growth processes and pigment complex recovered to the level of no irradiated plants and exceed it, indicating the induction of adaptive reactions in young corn plants. Conclusions. It was shown that the effect of a low dose of chronic UV-B irradiation caused a delay in growth processes and the formation of a photosynthetic complex with subsequent adaptation to stress in young corn plants. Keywords: UV-B irradiation, Zea mays L., photosynthetic pigments, adaptation.
The frequency of T-DNA integration during the genetic transformation of tobacco (Nicotiana tabacum L.)
Authors: Komisarenko A. G. , Mykhalska S. I. , Khrystan O. O.
Published: March 2020

Aim. Investigation of the is integration frequency of the genes and their components (elements of genetic constructions) in transgenic tobacco-regenerating plants. Methods. Agrobacterium-mediated transformation of tobacco (Nicotiana tabacum L.) in vitro. Results. There were obtained tobacco plants with integrated target and selective genes. It was found that a complete copy of T-DNA or its certain elements were embedded. Conclusions. The possibility of the integration of complete and incomplete DNA copies during in vitro Agrobacterium-mediated transformation of the Nicotiana tabacum were established. This event were observed both during manipulations with pBi2E plasmid with the double-stranded RNA PHD gene suppressor and pBi-OAT plasmid with OAT gene. The transformation frequency with the complete integration of the genetic construction was 91 %. The integration frequencies for various plasmids were: LBA4404 pBi2E – 78 %; AGLO pBi2E – 100 %; AGLO pBi-OAT – 94 %.

Keywords: Nicotiana tabacum L., Agrobacterium-mediated transformation in vitro, transgenic plants, T-DNA.
Microclonal propagation and callus induction of some species of Carlina L. genus
Authors: Kravets N. B. , Tulaidan N. V. , Mosula M. Z. , Drobyk N. M.
Published: March 2020

Aim. The aim of the research was to choose the conditions for microclonal propagation and obtain callus cultures from Carlina аcaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl plants in vitro. Methods. For microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia we used rosettes of 2–3-month specimens and planted them on semi-solid Murashige and Skoog (MS) medium with decreased macro- and microsalts concentrations (MS/2) supplemented with kinetin (Кin) (from 1–3 mg/l) and 0.1 mg/l of 1-naphthaleneacetic acid (NAA). For induction of callus formation, we used root, stem explants from С. acaulis, C. cirsioides and C. onopordіfolia, and planted them on nutrient media MS, MS/2, and Gamborg and Eveleigh (В5) supplemented with different concentrations of cytokinins – 6-benzylaminopurine (BAP) or Кin and auxins – 2.4-dichlorophenoxyacetic acid (2.4-D) or NAA and indole-3-acetic acid (IAA). Results. MS/2 medium supplemented with growth regulators of NAA and Кin were the most efficient to provide the formation of microclones. For C. сirsioides plants, this indicator was 6.6–6.8 rosettes per graft after 6 months of cultivation and for С. acaulis and C. onopordіfolia – 4.2–5.0 and 4.8–5.2 respectively. To raise the percentage of rooting for microclones of Carlina species, it was expedient to steep them preliminarily in the solution of indole-3-butyric acid (IBA) with 1000 mg/l concentration for a minute. Optimal for obtaining callus tissue from Carlina plants was nutrient medium MS supplemented with 3 mg/l IAA, 0.5 mg/l NAA and 0.5 mg/l Kin and MS/2 with 0.1 mg/l BAP and 0.5 mg/l 2.4-D; under such conditions the percentage of callus induction exceeded 90 % for all types of explants. Conclusions. There were chosen the conditions for microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia and worked out the schemes for enrooting obtained microclones in vitro. Capable of growing rapidly callus cultures from root and stem explants of the investigated plant species were obtained.

Keywords: Carlina аcaulis L., Carlina cirsioides  Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, microclonal propagation, callus induction.
Cloning of sequence of homologous crt-cluster in Streptomyces globisporus 1912-бп
Authors: Lukyanchuk V. V. , Polishchuk L. V.
Published: March 2020

Aim. The aim was to set influence to the additional homologous crt-cluster on carotenogenesis of cells of streptomycete recipient. Methods. For this purpose transformation by the hybrid plasmid pWC 9,6 was conducted. This plasmid contained the fragment of the crt-cluster sequence (9576 bp) of Crt+-mutant S. globisporus Crt4 in a Crt--recipient S. globisporus 1912-бп. To construct this hybrid plasmid, a fragment of PLR-copies of sequence of the crt-cluster of mutant S. globisporus 1912 Crt4 was cloned in the shuttle vector pWHM4 (6.6 kb). Insertion was done into unique restriction sites for endonucleases XbaI and HindIII in a polylinker of this vector. These endonucleases have not restriction sites into the crt-cluster sequence. Results. The plasmid pWC 9,6 (16.2 kb) that contains the crt-cluster sequence (9576 bp) of the Crt+-variant Crt4 of the strain S. globisporus 1912 was constructed. The plasmid successfully functions in the cells of both recipients (E. coli XL1 Blue and S. globisporus 1912-бп). It provides to them resistance to the corresponding antibiotics. The plasmid pWC 9,6 stably keeps its molecular size (16.2 kb). However, indisputable proofs of expression of the crt-clusters in transformants were not got. Conclusions. The plasmid pWC 9,6, that is able to transform and stably function in the cells of both recipient microorganisms (Streptomyces and E. coli) was constructed.

Keywords: crt-cluster, shuttle vector, cloning, resistance, PCR.
Optimization of conteining dermal coverages production for burn wound treatment at in vivo model
Authors: Matsevych L. L. , Papuga A. Ye. , Ruban T. P. , Beregova T. V. , Lukash L. L.
Published: March 2020

Aim. The aim was to estimate the influence of cell suspension quality on the therapeutic efficiency of cell-containing dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with different quality of cellular compound on the third degree burns of ICR line mice. In the negative control group animals were treated with fresh medium-containing gel, and in positive control – by gel containing high quality cell suspension. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing between positive control and both experimental groups. The decreasing of alive cell fraction in prepared gel within 5 hours has been shown as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells on the cell compound quality, in particular, from the viability and integrity of cells. These results are important for developing of clinical protocols using such cell‑containing dermal equivalents.

Keywords: burn wound, dermal equivalent, stem cells, skin substitute, tissue engineering.
Cell selection with heavy metal ions for obtaining wheat and maize forms tolerant to osmotic stresses
Authors: Sergeeva L. E. , Bronnikova L. I.
Published: March 2020

Aim. The obtaining tolerant to salt and water stresses wheat and maize variants via cell selection with barium and cadmium heavy metal cations were the idea. Barium cations disrupt the cell potassium transport. Cadmium cations affect the dehydrins. The maintenance of these parameters under stress conditions provides the plant tolerance. Methods. Modified media for initial selection were developed by the supplement of the lethal doses of heavy metal ions. Wheat and maize suspension cultures were plated on selective media. The relative fresh biomass growth was the marker of variant stress tolerance. Results. Ba-resistant and Cd‑resistant plant cell lines were obtained. Those variants were tested under salinity and water stress pressure at lethal for wild type cells doses. Cell lines demonstrated the combined tolerance to abiotic stresses. Conclusions. The cell selection with heavy metal ions – is a new approach for isolation cell variants with higher levels of osmotic stresses tolerance.

Keywords: cereals, osmotic stresses, cell selection with heavy metal ions, combined tolerance.
Assessment of structural peculiarities of glaziovianin A interaction with human α-, β and γ-tubulins
Authors: Karpov P. A. , Spivak S. I. , Rayevsky O. V. , Nyporko O. Yu. , Ozheredov S. P. , Blume Ya. B.
Published: March 2020

Aim. To determine the features of the ligand-protein interaction of glaziovianin A and human α-, β- and γ-tubulin. Methods. Protein and ligand spatial structure modelling (I-Tasser, Grid), molecular docking (CCDC Gold), molecular dynamics simulation (GROMACS). Results. Using the method of molecular docking in CCDC Gold ligand-protein complexes of glaziovianin A and human α-, β- and γ-tubulin were reconstructed. Studied ligand interactions in GTP/GDP-exchange and colchicine binding sites of different tubulin isotypes. The built ligand-protein complexes were studied using molecular dynamics simulations. Conclusions. Binding of glaziovianin A with human tubulin was confirmed exposing its derivatives as perspective tubulin effectors. The binding energies of ligand-protein interaction confirm higher affinity for β-tubulin molecules, and it was suggested that glazovianin A binding may occur at two alternative sites: GTP/GDP-exchange site and site of colchicine binding.

Keywords: tubulin, glaziovianin A, binding, antitumor activity.
Evolution of the stochastic and structural type ecosystem
Authors: Mikhyeyev A. N. , Protasov A. A.
Published: March 2020

The article attempts to apply a systematic approach to the consideration of types of existing systems to identify the specific evolution of ecosystems at different levels of organization. The conceptual main consideration of the problem of the specifics of the evolution of biotic systems at different levels of organization was the idea of the alternation of the types of their organization, namely, the statistical (C-system) and deterministic (D-system). For evolutionary transformations of C-systems (for example, populations), the influence of altered environmental factors is of primary importance, and for internal systems, for systems (for example, communities). The idea that the D-systems evolve according to the type of nomogenesis, and the C-system – selectogenesis is substantiated. Within the framework of the proposed isolation of different types of systems, the evolution of the biosphere probably proceeds according to the type of evolution of D-systems. Structural elements of the biosphere are diverse biogeocenoses, each of which represents a C-type system formed by the corresponding communities and elements of the environment.

Keywords: evolution, levels of organization, ecosystems, biosphere.
Genetic analysis of oleic acid сontent in the oil of maize on the basis of mutation Sugary-1
Authors: Tymchuk D. S. , Potapenko G. S. , Tymchuk N. F. , Myzhylko V. V.
Published: March 2020

Aim. Genetic analysis of oleate content in the carriers of maize  mutation su1. Methods. Experiments were conducted on a series of inbreds – carriers of mutation su1, as well as hybrids derived from their diallel and top cross crossings. Results. Inbreds – carriers of mutation su1 were notable as having the increased content of oleate, which was inherited by the type of incomplete dominance with the prevailing contribution of additive effects to the variance. Conclusions. Increasing the content of the oleic acid glycerides in the carriers of mutation su1 caused by the spatial coupling of the locus su1 with the oleate – encoding locus, the effect of which can be modified by the polygenic complex.

Keywords: Zea mays L., mutation su1, oleic acid, genetic analysis.
Susceptibility of siberian hybrids new races of zarazhi
Authors: Hablak S. G. , Abdullaeva Ya. A. , Ryabovol L. O. , Ryabovol Ya. S.
Published: March 2020

Aim. A study of the racial composition of broomrape on sunflower crops in the northern part of the Steppe of Ukraine. Methods. Assessment of the resistance of hybrids and sunflower test lines to broomrape was carried out by a modified soil method. Results. The broomrape parasitizing on the fields of sunflower has a high degree of virulence, which overcomes the immunity of the best hybrids of domestic and foreign breeding, resistant to the E, F and G races of this parasite. Conclusions. The emergence of new very aggressive broomrape races (E, F and G) indicates the important need to solve the problem of creating breeding material resistant to new races of this parasitic plant. Intensive with the accumulation of parasite races E, F and G in the sunflower crops is associated with the disruption of crop rotations and the saturation of fields with hybrids of this culture, resistant mainly to 4 (D) and 5 (E) races of the parasite.

Keywords: Orobanche сumana Wallr., race, sunflower, hybrid, root system, root allocation, srigolactones.
Mutation 6174delT in the BRCA2 gene in men with prostate cancer in Ukraine
Authors: Gorodetska I. V. , Serga S. V. , Stakhovsky E. O. , Kononenko O. A. , Stakhovsky O. E. , Pikul M. V. , Demydov S. V. , Kozeretska I. A.
Published: March 2020

Aim. Mutations in BRCA1/2 genes are known to increase the risk of human breast cancer, pancreatic cancer and prostate cancer. In Ukraine only mutations in woman BRCA1/2 were studied, thus the situation with these diseases related to disturbance of these genes in Ukrainian men remains poorly explored. 6174delT is a frameshift mutation and is results in stop of transcription and translation of the functional protein BRCA2. Methods. Genomic DNA was purified from peripheral blood.We performed PCR analysis of 116 DNA from men with prostate cancer in order to detect presence of mutation in BRCA2 gene (6174delT). To detect the mutation by mutagenically separated PCR, we used two PCR reactions. The first reaction used one general primer and one primer specific to the mutation, and the second reaction was with one general primer and one primer specific to the wild type allele. Results. Among screened samples no mutations in the BRCA2 gene were found in prostate cancer patients. Conclusions. Our results suggest that the incidence of this event is not more than 0.9% among men with prostate cancer inUkraine. Keywords: mutation, 6174delT, BRCA2, prostate cancer, Ukraine.
Microsatellite alteration and metylation of RASSF1 gene in renal cell carcinoma
Authors: Dubrovska H. V. , Onyshchenko K. V. , Pereta L. V. , Kashparova O. V. , Grygorenko V. M. , Skrypkina I. Ya.
Published: March 2020

Aim. It becomes more and more relevant to study as the promising diagnostic and prognostic markers the epigenetic and genetic changes that determine the malignant transformation of cells and biological behavior of tumors. The purpose of our research was to study the loss of heterozygosity of the RASSF1 gene and the status of methylation of the locus of the RASSF1A gene in malignant tumors of the kidneys and to assess the potential for its use as a marker in clinical practice. Methods. The determination of the allele imbalance in the RASSF1 gene locus was performed by PCR using highly polymorphic markers D3S966, D3S1568 for renal cancer (RC) in paired samples of tumor and conditionally normal tissue and the PCR products detection in polyacrylamide gels. The epigenetic variability analysis of the RASSF1A gene was performed by using methyl-specific PCR, which was preceded by bisulfite DNA treatment. The statistical significance of the differences between the study groups was analyzed by using F-test and the U-test. Results. Our studies showed the predictive value of inactivation of RASSF1A in RC. The methylation level of the genomic DNA of the RASSF1A loci of the RASSF gene was 72 %. The loss of the heterozygosity of the RASSF1 gene in this type of oncological disease was about 35.4 %. Conclusions. The results may indicate that the gene RASSF1can become a candidate for inclusion in the prognostic system of clinical course of the disease.

Keywords: kidney cancer, epigenetic changes, methylation, loss of heterozygosity, RASSF1.
Genetic features of the glutathyon system of long livers of Carpathian region with comorbid pathology: arterial hypertension and osteoarthrosis
Authors: Kozoviy R. V. , Kovalchuk L. Ye. , Erstenyuk H. M.
Published: March 2020

Aim. To study the polymorphism of GSTM1, GSTT1 genes. To study the activity of enzymes encoding these genes in long-livers with arterial hypertension (AG) and osteoarthritis (OA). Methods. The determination of the frequency of polymorphous variants of gene deletions GSTT1 and GSTM1 and the activity of glutathione S-transferase (GST), glutathione reductase (GRD), glutathione peroxidase (GPO) was performed in long-livers, who were separated depending on the presence AG and OA. Results. In the group of patients with hypertension and OA genotype GSTM1«+»/GSTT1«+» met the GSTM1«+»/GSTT1«-» 3.0 times more often. The genotype GSTM1«-»/GSTT1«+» in comparison with GSTM1«-»/GSTT1«-» in this study group was 3.17 times. The study of GPO, GRD, and GST enzymes has shown a tendency to depend on its level from the health of long-livers. Conclusions. The advantage of genotypes with the active form of the gene GSTT1«+» was spotted among healthy long livers and patients with hypertension and OA. It indicates a better functioning of the antioxidant defense system. The activity of the enzymes of the glutathione system GPO, GRD, and GST was higher in healthy long-livers livers than in patients with comorbid pathology. Keywords: glutathione system, longevity, arterial hypertension, osteoarthritis.
Cytogenetic aspects instability to the genome in newborns from different ecological districts of Ivano-Frankivsk region
Authors: Kocherha Z. P. , Kovalchuk L. Ye.
Published: March 2020

Aim. Оf the research work was to establish the frequency and spectrum of chromosomal aberrations, associations of acrocentric chromosomes in infants from different ecological regions of Ivano-Frankivsk region. Methods. Metaphase plates of 187 infants from different ecological districts have been analyzed with the aim to determine the frequency and spectrum of chromosomal aberrations, associations of acrocentric chromosomes in newborns. Results. The obtained results of the frequency of associations of acrocentric chromosomes correlated with the frequency indices of chromosomal aberrations (r correlated from 0.68 to 0.84), that proved the negative influence of ecological living conditions on immunogenetic status and adaptive human capabilities. Conclusions.It was determined that the total number of chromosomal aberrations in newborns from districts of ecological comfort was 2.0 and 2.3 times lower as compared to newborns from zones with chemical and radiation contamination. The frequency of cells with associations of acrocentric chromosomes was 7.8 % higher in children from zones of chemical contamination and 9.9 % from zones of radiation pollution as compared to the children from districts of ecological comfort.

Keywords: chromosomal aberrations, associations of acrocentric chromosomes, newborns, ecological zones.
EMT-mechanizm induces the leukemic stemness phenotype in myeloid leukemias
Authors: Shvachko L. P.
Published: March 2020

Aim. To study the targeted expression EMT-induced markers N-cadherin, Snail and Twist in patients with the chronic and acute myeloid leukemias. Methods. RT-PCR, electroforesic in agarose gel, TotalLab v. 2.01 densitometry. Results. Have been investigated the relative levels of mRNA expression of N-cadherin and transcriptional factors Snail and Twist, associated with epithelial-to-mesenchymal induction (EMT) in patients with the essential polycytemia (EP), the chronic mieloid leukemia CML), the acute myeloid leukemia (AML) and the acute lymphoblastic leukemia (ALL). Conclusions. Have been highlighted the EMT stemness mechanism in Leukemic stem cell progression.

Keywords: the epithelial-to-mesencymal transition (EMT), EMT-inducer marker, N-cadherin, Snail, Twist, myeloid leukemias, leuklemic stem cell progression.
The determination of the stability of nitrogen-fixing microorganisms of the soil of Ecuador to toxic metals CrO42–, Ni2+, Cu2+
Authors: Bielikova O. Yu. , Matvieieva N. A. , Yastremskaya L. S. , Tashyrev A. B.
Published: March 2020

Aim. The aim of the work was to determine the stability of nitrogen-fixing microorganisms isolated from the rhizosphere of bromelia (Ecuador), to the effect of toxic metals. Methods. Microorganisms were isolated on the Ashby nutrient medium. The selected strains were cultured on a medium with Cu2+ (Cu (ІІ) citrate) from 50 to 500 mg/l by cation in steps of 50; Ni2+ (NiCl2) from 20 to 200 mg/l by cation in steps of 20; Cr (VI) (K2CrO4) from 20 to 100 mg/l with Cr (VI) in increments of 20. The growth of microorganisms in the presence of metals was characterized by the maximum permissible concentration (MPC) of metals, duration of lag phase and the number of colony-forming units (CFU) of nitrogen-fixing microorganisms with increasing concentration of toxic metals. Results. The selected dominant nitrogen-fixing microorganisms from the soil of Ecuador were resistant to toxic metals (Cu2+, Ni2+, Cr (VI)) in high concentrations. It was found that MPC for microbial communities of nitrogen-fixing microorganisms were: 40 mg/l Cr (VI), 300 mg/l Cu2+ and 100 mg/l Ni2+. Conclusions. It was shown that the selected dominant nitrogen-fixing microorganisms from the Ecuadorian soil were resistant to toxic metals (Cu2+, Ni2+, Cr (VI)) in high concentrations, which in 4–30 times exceed the damage or bactericidal concentrations for the majority of known organophosphate microorganisms of natural ecosystems.

Keywords: metalresistance, nitrogen-fixing microorganisms, Cu2+, Ni2+, Cr (VI).
Use of DNA markers for studying the genetic polymorphism of the bread wheat (T. aestivum Desf.) genotypes of Azerbaijan
Authors: Hajiyev E. S. , Akparov Z. I. , Aliyev R. T. , Babayeva S. M. , Izzatullayeva V. I. , Abbasov M. A. , Mamedova A. D.
Published: March 2020

Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected from different regions of Azerbaijan. Methods. Molecular-genetic. Results. The mean polymorphism level for the studied genotypes of bread wheat, using 8 ISSR markers, was 72 %. An index of genetic diversity calculated for the studied collection of T. aestivum specie was equal to 0.91. Among the bread wheat accessions, the highest genetic diversity was established for botanical variety var. erythrospermum (GDI=0.90). Conclusions. Primers UBC 112, UBC 841, UBC 857 and UBC 873 were identified to be more effective in identification of bread wheat accessions. As a result of the GGE biplot analysis, a trait collection was created by selecting 20 bread wheat samples with broad genetic variation. А core collection of bread wheat was created, which coversthe most distant genotypes and represents the genetic diversity of all 110 accessions studied.

Keywords: Triticum aestivum Desf., genetic polymorphism, ISSR markers.
Dependence of grain productivity of the main shoot ear on the stem dry matter weight in varieties of winter wheat
Authors: Zborivska O. V. , Priadkina G. O. , Sokolovska-Sergienko O. G. , Makharinska N. M.
Published: March 2020

Aim. Estimation of the mass of dry matter of the stem of the main shoots of different varieties of winter wheat in the phases of flowering and full ripeness as a criterion for the grain productivity of its ear in years with different meteorological conditions. Methods. Field, morphometric, mathematical and statistical. Results. The mass of dry matter of the stem of the main shoots of winter wheat varieties was studied in years that differed significantly in weather conditions in the flowering phase and at full grain maturity. It was established that the mass of dry matter of the stem of the main shoot at the flowering and the full grain maturity varied significantly, respectively 0.6–1.2 g and 0.5–1.0 g in 2014, and 1.0–2.0 g and 0.8–1.5 g in 2016 depending on the variety, and under different growing conditions. Limits of variation of grain yield of the ear in 2014, ranged from 0.9 to 2.2 g and from 1.5 to 2.5 g in 2016. It was established that at the flowering, the correlation between the mass of grain from the head and the mass of the dry substance of the stem was significant at 99 % probability under growing conditions (r = 0.8–0.9). Conclusions. It is shown that grain productivity of main shoot in winter wheat is related to the ability of its stem to store assimilates during the vegetative period. It was established that the mass of the dry matter of the stem of the main shoots in the flowering phase can serve as a criterion for assessing its grain productivity.

Keywords: Triticum aestivum L., mass of stem, grain yield.
Biochemical analysis and assessment of modern monogamous varieties of hemp Cannabis sativa L. by the content of cannabinoid substances
Authors: Polyakova A. S.
Published: March 2020

Aim. Defining the peculiarities of forming the cannabinoid substances in modern monogamous varieties of hemp is of both theoretical and practical importance. Methods. Modern monogamous varieties of hemp bred in Hlukhiv, Zolotonosha and specimens receive from France; were used for the chromatographically analysis of «damp» and dried in the shadow plant samples was conducted by the method of thin layer chromatography (TLC). Results. It was founding, that in the initial phase of growth and development the Ukrainian hemp varieties with the practical absence of neutral compounds and the French varieties with the sufficient content of these substances formed only natural acids. During the generative phase of growth and development, the varieties from France formed not only natural acids but also neutral compounds. The Ukrainian varieties demonstrated only insufficient increase of natural acids. Conclusions. In the initial phase of growth and development, the investigated hemp varieties containing both large and insignificant amount of cannabinoid substances formed only natural acids. Separate plants demonstrated the decrease of natural acids up to their total absence, which proves their biological interconnections and possible forming cannabidiol, tetrahydrocannabinol and сannabinol by way of fermentative decarboxylation the respective acids.

Keywords: Cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA).
Genetic diversity of spring common wheat varieties at storage protein loci
Authors: Sobko T. O. , Sirant L. V. , Lisova G. M.
Published: March 2020

Aim. The aim of this study was to investigate genetic diversity of storage protein loci in spring common wheat varieties licensed for growing in Ukraine in 2017 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the gliadin loci Gli-1 and the high-molecular-weight glutenin subunit loci Glu-1. Results. Genotypes at the gliadin loci Gli A1, Gli B1, Gli D1 and glutenin loci Glu A1, Glu B1, Glu D1 were identified in 31 spring common wheat. The wheat-rye translocation AL/1RS was identified in two varieties Etyud and Struna mironivsyka. Differences in frequencies of alleles at storage protein loci were revealed. Conclusions. The high level of allelic variation was observed at the Gli-1 loci in spring common wheat varieties. Predominant alleles (one or two per locus) were revealed: Gli A1f, Gli A1а, Gli B1e, Gli D1b, Gli D1і, Gli D1f , Glu А1а, Glu Bс , Glu D1d, Glu D1a.

Keywords: Triticum aestivum L., spring wheat, storage protein.
Actinomycetes of Juniperus excelsa Bield. rhizosphere – antagonists of phytopathogenic microbiota
Authors: Tistechok S. I. , Syrvatka V. Ya. , Fedorenko V. O. , Gromyko O. M.
Published: March 2020

Aim. Phytopathogenic microorganisms are one of the main causes of agricultural productivity losses. Thereby, the goal of this study was to evaluate actinomycetes strains, isolated from Juniperus excelsa Bield. rhizosphere, antagonistic activity against plant pathogenic bacteria and fungi. Methods. In this study we used microbiological methods for isolation actinomycetes from rhizosphere. Antagonistic activity was evaluated by using the dual culture method. Results. 372 actinomycete stains were isolated from J. excelsa Bield. rhizosphere. More than 60 % actinomyces isolates showed antibacterial activity against to lest one of the tested phytopathogenic bacteria genus Pseudomonas, Pectobacterium, Agrobacterium, Erwinia, Xanthomonas and 20.5 % of the tested phytopathogenic fungi genus Aspergillus, Alternaria, Fusarium, Botrytis. Only 2 strains had antagonistic activity to the all of the tested microorganisms and 62 strains, which had antagonistic activity to the one test-microorganism. Conclusions. Actinomicetes of J. excelsa Bield. rhizosphere are source for bioactive compounds against phytopatogenic microorganisms and showed good biotechnology potential. These results are the first step to the screening new biopesticides for controlling phytopatogenic diseases in plan. Keywords: actynomicetes, phytopathogens, biocontrol.
Polymorphism upstream the coding region of the Ppd-D1 gene in Aegilops tauschii Coss.
Authors: Chebotar G. O. , Sechnyak V. E. , Yurganova T. Yu. , Chebotar S. V.
Published: March 2020

Aim. Evaluate time of heading and flowering in Aegilops tauschii Coss. accessions, donor of  the bread wheat D genome, that are differentiated by polymorphism at upstream the coding region of the Ppd-D1 gene. Methods. NA extraction, allele-specific PCR, electrophoresis in polyacrylamide gel, two-factor analysis of variance. Results. We have found polymorphism upstream the coding region of Ppd-D1 gene with the help of the allele-specific PCR with primers to the Ppd-D1b allele in the accessions from the collection of Ae. tauschii Fragment 414 bp was detected in the samples: k-78, k-396, k-529, k-531, k-1144, k-1162, k-1235, k-1273, k-1283, fragment 429 bp have been revealed in the samples  k-187, k-968, k-2178, and 453 bp – in the samples k-80, k-2358, Tetra tauschii. The results of a three-year field experiment showed a significant effect of the detected polymorphisms on the time of heading and flowering. Conclusions. Among the Ae. tauschii accessions by allele-specific PCR with primers to the Ppd-D1b allele have been tested amplification fragments 414 bp, 429 bp and 453 bp. Allele Ppd-D1a was not found in the collection of Ae. tauschii accessions. Samples of aegilops that are carrier of insertions 24 + 15 bp (for which the fragment 453 bp was detected by allele-specific PCR) – were characterized by earlier heading and flowering time in certain years.

Keywords: time of heading and flowering, Ppd-D1 gene, Aegilops tauschiі Coss.
Mercury-resistant bacteria in Antarctic ecosystems
Authors: Sioma I. B. , Hovorukha V. M. , Tashyrev O. B.
Published: March 2020

Aim. The research was focused on the assessment of the possible resistance of Antarctic microorganisms to mercury ions. Methods. Conventional microbiological methods of introducing of decimal dilutions to agar medium. Results. Microorganisms resistant to Hg2+ in high concentrations (up to 500 mg/l) were discovered among samples from Antarctica. The majority of Hg2+-resistant microorganisms was found in moss-lichen and soil ecosystems. The least of Hg2+-resistant microorganisms was found in organics-rich humus ecosystems. Conclusions. In Antarctic microbiocenoses were discovered for the first time microorganisms resistant to mercury in “bactericidal” concentrations in spite of absence of this element in the natural ecosystems. Apparently, the genome of these microorganisms contains genes that provide the mechanisms of microbial resistance to toxic mercury.

Keywords: mercury, metal resistance, Antarctic microorganisms.
Puffing activity in Drosophila melanogaster Meig. political chromosomes after exposure to microwave radiation
Authors: Sheyka M. N. , Strashnyuk V. Yu.
Published: March 2020

Aim. The aim of the work was to study the effect of microwave radiation of varying intensity on the polytene chromosomes puffing activity in larvae salivary glands of Drosophila melanogaster. Methods. The wild type outbred strain Oregon-R was used as the material. Microwave radiation with a frequency of 36.64 GHz and a power density of 0.1 and 1 W / m2 was used. Exposure to microwaves was applied in early embryogenesis after 3-hour oviposition. Exposure time was 30 sec. The puff sizes were studied on the squashed preparations of larvae salivary glands stained with acetoorcein. Dimensions of four puffs were investigated^ 2B5-6 (X chromosome); 62E, 71CE and 72CD (chromosome 3L). The measurements were carried out using an ocular-micrometer. Results. There were no significant changes in the size of the puffs in any of the four loci studied, regardless of the applied power density. Conclusions. Microwave radiation in early embryogenesis at a frequency of 36.64 GHz, a power density of 0.1 and 1 W/m2, and an exposure of 30 sec does not have a significant effect on the puff sizes in the Drosophila polytene chromosomes.

Keywords: Drosophila melanogaster Meig., giant chromosomes, puff sizes, non-ionizing radiation.
Studying the radioprotective properties of some phytoextracts from Azerbaijan pharmaceutical flora
Authors: Shamilov E. N. , Abdullaev A. S. , Shamilli V. E. , Azizov I. V.
Published: March 2020

Aim. The aim of this work is to evaluate the radioprotective properties of the extract from the flowers of calendula officinalis (Calendula officinalis L.) alone and phytoextracts (CDY) consisting of calendula officinalis (Calendula officinalis), dandelion officinalis (Taráxacum officinále) and yarrow (Achillea millefolium) obtained from medicinal flora of Azerbaijan. Methods. The medicinal plants included in the collection were dried and the extract obtained in a water-alcohol mixture. The extracts were injected intraperitoneally in mice at a concentration of 300 mg / kg 30 min before irradiation at a dose of 9 Gy. Results. It was found out that the extract of calendula possesses radioprotective activity (55%), somewhat inferior to the activity of the protector-standard of cystamine (80%), and phytoextracts (CDY) decreasing the lethal effect of ionizing radiation, has radioprotective activity (62%) and increases survival and life expectancy lethally irradiated mice more than the extract of calendula (55%) and less cystamine (80%). Conclusions. The data obtained by us testify to the prospects of using the extract of calendula separately and in a complex of extracts of dandelion and yarrow in the development of medicinal compositions intended for preventive purposes and in order to prevent local radiation injuries.

Keywords: mice, survival, irradiation, extract, cystamine.
Hybride necrosis genes in bread wheat varieties (Triticum aestivum L.) of Ukraine
Authors: Galaev A. V. , Galaeva M. V.
Published: March 2020

Hybrid necrosis is the gradual premature death of leaves or plants in certain F1-2hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, necrotic genotypes in most varieties of Ukrainian breeding have not been identified. Aim. This study was conducted to determine the necrotic genotypes in varieties Ukrainian breeding of different regions. Methods. Microsatellite analysis, PAAgel-electrophoresis. Results. 150 genotypes of bread wheat varieties from Ukrainian breeding were identified by loci Xbarc74-5В and Xbarc55-2В closely linked to hybrid necrosis genes Ne1 and Ne2, respectively. Conclusions. The most common in the South of Ukraine is the genotypes Ne1wNe2w/m and Ne1mNe2w/m were revealed. The most of varieties in the North of Ukraine had the genotypes ne1Ne2ms. These genotypes can have breeding and adaptive value for specific geographical conditions.

Keywords: Triticum aestivum L., hybride necrosis genes Ne1 and Ne2, microsatellite analysis.