bluuebunny/crossref_metadata_2025 huggingface dataset:
A negative stiffness inertial nonlinear energy sink
Authors: Zhang Zhen , Gao Yuan , Zhang Ye-Wei , Fang Bo
Published: October 2022

In this paper, a novel negative stiffness inertial nonlinear energy sink (NSI-NES) is proposed. The main structure is simulated as a single-degree-of-freedom linear oscillator. Newton's second law is applied to derive the kinematic equations of the coupled system. Based on the Runge–Kutta numerical solution, the complex dynamical behaviors of the system coupled with the NSI-NES are explored. Moreover, the quasiperiodic solution exhibited a strongly modulated response (SMR). The steady-state response of the system is obtained using the Runge–Kutta and harmonic balance methods and is cross-corroborated. Compared with the inertial nonlinear energy sink (I-NES) and the positive stiffness inertial nonlinear energy sink (PSI-NES), the damping effects of the NSI-NES are highlighted based on various excitations. The results show that the NSI-NES has a damping effect of up to 90%. In addition, the effect of the NSI-NES parameters on the damping effect is discussed. In general, the negative stiffness element can significantly improve NES performance. Therefore, this study promotes the application of negative stiffness and inerter in engineering.
Screening for climate adaptative loci using seascape genomics resolves phenotypic variation in heat tolerance of the coral Acropora millepora.
Authors: DENIS Hugo , Selmoni Oliver , Gossuin Hugues , Jauffrais Thierry , Lecellier Gaël , Berteaux-Lecellier Véronique
Published: October 2022

One of the main challenges in coral reef conservation and restoration is the identification of coral populations resilient under global warming. Seascape genomics is a powerful tool to uncover genetic markers potentially involved in heat tolerance among large populations without prior information on phenotypes. Here, we aimed to confirm the role of several candidate heat associated loci identified using this method in driving the phenotypic response of Acropora millepora from New Caledonia to thermal stress. We subjected 7 colonies to a long-term ex-situ heat stress assay (4°C above the Maximum Monthly Mean) and investigated their physiological response along with their Symbiodiniaceae communities and genotypes. Despite having similar thermal histories and associated symbionts, these conspecific individuals differed greatly in their tolerance to heat stress. More importantly, the clustering of individuals based on their alleles at the candidate loci was able to resolve most of the phenotypic variation in heat tolerance. Colonies harboring a higher proportion of beneficial alleles sustained on average lower mortality, higher Symbiodiniaceae/chlorophyll concentrations and photosynthetic efficiency under prolonged heat stress. Together these results support the relevance of seascape genomics to reveal candidate loci for heat adaptation in corals and develop predictive models of heat tolerance.
The use of blockchain as an efficient medical record-keeping system in s health care
Authors: Ngutshane Dimakatso , Magida Ayanda
Published: October 2022

Background
Blockchain technology has sparked widespread interest, with applications ranging from data management to financial services and the healthcare business. The use of blockchain technologies to deliver safe and secure health data management has sparked much attention. Blockchain is transforming traditional healthcare methods into a more dependable means of treatment and diagnosis by providing secure and safe data sharing. Blockchain could be a technology that, in the future, helps with tailored, genuine, and secure healthcare by combining all of a patient's real-time clinical data and organising it in an up-to-date secure healthcare setting. This study explores the feasibility of adopting blockchain for effective patient medical records management in South Africa’s healthcare industry.
Methods
Qualitative interviews were conducted with six medical systems analysis experts and probed deeper for their viewpoints and a greater understanding of the field. Finally, a thematic analysis was conducted under the pretext of research, in which the overlaps of various specialists were examined.
Findings
Three themes emerged from the findings: misconceptions about blockchain technology in healthcare, Legacy Filing Systems, and the blockchain's benefits. All participants view blockchain as providing transparency, traceability, and immutability, decreasing risk. Hospitals and healthcare professionals must first demonstrate a willingness to adopt and accept blockchain technology and resolve any obstacles.
Conclusion
The findings suggest blockchain could be a good solution for managing patient medical records. However, the South African healthcare sector is not ready to utilise blockchain, and education is required.
Citrus Pulp in Broilers Feeding (1-42 Days)
Authors: Diaz-Vargas Mayra , Murakami Alice Eiko , Zanetti Leonardo Henrique , Lipori Humberto , Sakamoto Márcia Izumi , Nascimento Guilherme
Published: October 2022

The aim of this study to determine the chemical composition, the metabolizable energy, and digestibility of citrus pulp; furthermore, evaluate the effect of using this product on performance, carcass yield, and blood parameters of broilers from 1 to 42 days of age. In the first experiment, it was using a total of 108 21-days-old male Cobb 500 birds weighing 926 g ± 50g was distributed in a completely randomized design with three treatments (basal diet and basal diet + 10 or 20% of testing feed), and six replicates of six birds each. In the second experiment, it was using a total of 966 1-day-old Cobb 500 male broiler chicks was distributed in a completely randomized design with six treatments (0, 2, 4, 6, 8 and 10% inclusion of citrus pulp) and seven replicates of 23 birds each. The citrus pulp presented 1311 kcal of AME/kg DM, 4.62% crude protein, 30.85% neutral detergent fiber, 36.93% acid detergent fiber and 23.45% pectin. The citrus pulp can be used up to 10.0% of inclusion in broiler diets in the period from 1 to 42 days old, with no damage to performance, carcass yield, blood parameters and with better economic indices.
Combined Metabolic Activators Improve Cognitive Functions in Alzheimer’s Disease Patients: A Randomised, Double-Blinded, Placebo-Controlled Phase-II Trial
Authors: YULUG BURAK , ALTAY OZLEM , LI XIANGYU , HANOGLU LUTFU , CANKAYA SEYDA , LAM SIMON , VELIOGLU HALIL AZIZ , YANG HONG , COSKUN EBRU , IDIL EZGI , NOGAYLAR RAHIM , OZSIMSEK AHMET , BAYRAM CEMIL , BOLAT ISMAIL , ONER SENA , TOZLU OZLEM OZDEMIR , ARSLAN MEHMET ENES , HACIMUFTUOGLU AHMET , YILDIRIM SERKAN , ARIF MUHAMMAD , SHOAIE SAEED , ZHANG CHENG , NIELSEN JENS , TURKEZ HASAN , BOREN JAN , UHLEN MATHIAS , Mardinoglu Adil
Published: October 2022

Background Alzheimer’s disease (AD) is associated with metabolic abnormalities linked to critical elements of neurodegeneration. We recently administrated Combined Metabolic Activators (CMA) to the AD rat model and observed that administration of CMA activated the mitochondrial functions and eventually improved the AD-associated histological parameters in the animals. CMA consists of NAD + and glutathione precursors and includes L-serine, nicotinamide riboside, N-acetyl-L-cysteine, and L-carnitine tartrate. Methods Here, we designed a randomised, double-blinded, placebo-controlled phase-II trial and studied the effect of CMA administration on the global metabolism of AD patients. The primary endpoint was on the difference in cognitive and daily living activity scores between the placebo and the treatment arms. The secondary aim of this study was to evaluate the safety and tolerability of CMA. We also performed a comprehensive human plasma metabolome and proteome analysis. Results Based on our analysis, we showed a significant decrease of ADAS-Cog scores on Day 84 vs Day 0 (p = 0.00001, 29% improvement) in the CMA group. Moreover, there was a significant enhancement (p = 0.0073) in ADAS-Cog scores between CMA and placebo groups in patients with higher ADAS-Cog scores. Improved cognitive functions were endorsed with relevant hippocampal volumes and cortical thickness alterations. Moreover, the plasma levels of proteins and metabolites associated with NAD + and glutathione metabolism are significantly improved after treatment. Conclusion In conclusion, our results show that treating AD patients with CMA leads to enhanced cognitive functions associated with the improved metabolome, proteome and structural neuroimaging parameters, suggesting a role for such a therapeutic regimen in treating patients, especially with severe AD. Trial registration: ClinicalTrials.gov NCT04044131 Registered 17 July 2019, https://clinicaltrials.gov/ct2/show/NCT04044131
Analysis of resident mental health and its influencing factors: a single-centre cross-sectional study
Authors: Luo Jiahui , Yang Lingli , Zhang Changlin , Zhang Huaxia , Yu Jiale , Bao Lingbo , Liu Fang
Published: October 2022

Background: With a high rate of positive psychological problem detections and as one of the main reasons why residents are unable to complete the required amount of training, the mental health of residents has attracted more attention in recent years. This study aims to comprehend the psychological well-being of Chinese residents and investigate the factors affecting their mental health.
Methods: A self-administered questionnaire containing the General Information Questionnaire and the Depression-Anxiety-Stress Scale (DASS-21) was used in a cross-sectional study at the First Affiliated Hospital of Kunming Medical University to survey residents and compare differences in the mental health status of residents with various characteristics. Univariate analyses and logistic regression analysis were performed to identify said factors.
Results: Out of the 386 valid questionnaires that were collected, univariate analyses showed that daily sleep time, weekly frequency movement, income, training department, gender impact on the resident’s mental health(P<0.05). Logistic regression analysis showed that sleeping less than 6 hours, internships in other departments, and male trainees are associated with the level of mental health.
Conclusion: These results imply that residents' mental health issues may be closely related to residency training characteristics and the training environment, or that routine psychological therapy for important targets will improve residents' mental health and guarantee successful training completion.
Uremic toxins, inflammation biomarkers and hepcidin in older CKD patients switched from high flux HD to OL-HDF
Authors: Fanchini Vanessa , Morales Armando , Silva Luana Cristina Almeida , Gonçalves Thiago José Martins , Kussi Fernanda , Nakao Lia Sumie , Elias Rosilene Motta , Dalboni Maria Aparecida
Published: October 2022

Anemia is a common complication that is associated with mortality in patients with chronic kidney disease (CKD). Despite the use of erythropoiesis-stimulating agents (ESAs) to correct anemia, some patients are hyporesponsive due to the state of micro-inflammation caused by uremic toxins and hepcidin, a hormone that plays a central role in iron homeostasis. Hemodiafiltration (OL-HDF) has been associated with better clearance of uremic toxins, such as indoxyl sulfate (IS), p-cresyl sulfate (PCS) and indole acetic ascorbic (IAA) than conventional hemodialysis (HD). The aim of the study was to evaluate the effect of OL-HDF treatment on the concentration of IS, PCS, IAA, hepcidin and inflammatory biomarkers in CKD patients. Thirty-one patients (> 65 years old) incident in OL-HDF were followed for 6 months. IS, PCS, IAA, biochemical parameters, hepcidin and inflammatory cytokines were evaluated at baseline and after 6 months. We observed a significant decrease in IS and CRP plasma concentration, an increase in hemoglobin and hematocrit after 6 months of treatment with OL-HDF (p <0.05). This prospective observational study demonstrated that OL-HDF was capable to reduce IS and CRP in older patients. Whether this reduction may have an impact on clinical outcomes must be investigated in a future study.
Polyglycerol-amine Covered Nanosheets Target Cell-free DNA to Attenuate Acute Kidney Injury
Authors: Wu Kefei , Lu Xiaohui , Li Yi , Wang Yating , Liu Ming , Li Hongyu , Li Huiyan , Chen Wei , Liu Qinghua , Zhou Yi , Tu Zhaoxu , Mao Haiping
Published: October 2022

Increased levels of circulating cell-free DNA (cfDNA) are associated with poor clinical outcomes in patients with acute kidney injury (AKI). Scavenging cfDNA by nanomaterials has been regarded as a promising remedy for cfDNA-associated diseases. Nevertheless, the nanomaterials-based cfDNA scavenging tactic has not yet been reported in AKI treatment. Herein, polyglycerol-amine (PGA) covered MoS2 nanosheet with medium size (M-PGA-M) was erected to bind negatively charged cfDNA for AKI treatment. M-PGA-M exhibited excellent cfDNA scavenging efficacy and low cytotoxicity, which effectively reduced serum cfDNA levels, dominantly accumulated in kidney to inhibit neutrophil extracellular trap formation and inflammation, thereby alleviating LPS-induced AKI in mice. The renoprotective effects of M-PGA-M were superior to corresponding polymer PGA attributed to its unique two-dimensional nanostructure. Further, M-PGA-M showed to decrease serum cfDNA from AKI patients. Collectively, M-PGA-M could serve as a potent cfDNA scavenger, suggesting its potential application in treating AKI and other cfDNA-associated diseases. Teaser Scavenging cell-free DNA (cfDNA) with polyglycerol-amine (PGA)-covered MoS2 nanosheets may represent a novel treatment for AKI.
DNMT3A promotes the proliferation and metastasis of esophageal squamous cell carcinoma via upregulating HDAC9
Authors: Pan Minghong , Feng Yingtong , Ding Peng , Ning Jiayi , Wang Yuanyong , Wang Zhaoyang , Duan Hongtao , Shao Changjian , Jiang Menglong , Ren Xiaoya , Lu Di , Yang Hanyi , Zhou Xin , Zhang Yimeng , Han Jing , Ma Zhiqiang , Yan Xiaolong
Published: October 2022

Background Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide and is characterized by high morbidity and mortality. However, the detailed molecular mechanisms underlying malignant progression of ESCC remain unclear. Methods 140 patients with esophageal squamous cell carcinoma who underwent surgery were enrolled in this study. Clinicopathologic characteristics and Kaplan–Meier survival analysis were performed to investigate DNMT3A expression and HDAC9 expression. Cell functional experiments were implemented both in vivo and in vitro to investigate the effects on ESCC cell proliferation and metastasis. Recombinant lentivirus–meditated gene overexpression or knockdown showed that HDAC9 participated in DNMT3A-mediated ESCC progression in vivo and in vitro, and this was further confirmed by HDAC inhibitor (SAHA) and immunohistochemical. Results We first demonstrated that DNMT3A expression was significantly higher in ESCC tissues than in corresponding adjacent non-cancerous tissues, and high DNMT3A expression was associated with poor prognosis. Then we confirmed that DNMT3A promoted proliferation and metastasis of ESCC cells in vivo and in vitro, suggesting that DNMT3A may be a promising therapeutic target for preventing esophageal cancer. Additionally, we found that DNMT3A up-regulated HDAC9, and HDAC9 overexpression significantly reversed the inhibitory effect caused by DNMT3A gene knockdown. In addition, we further confirmed the involvement of HDAC9 in DNMT3A-mediated carcinogenesis using HDAC inhibitor (SAHA). At last, we also validated HDAC9 expression was significantly higher in ESCC tissues than in corresponding adjacent non-tumour tissues, and high HDAC9 expression was positively associated with poor prognosis. Interestingly, Spearman correlation analysis confirmed a significant positive correlation between DNMT3A and HDAC9 expression. and ESCC patients with simultaneous DNMT3A and HDAC9 expression have the worst prognosis. Conclusions These results suggest that HDAC9 is involved in DNMT3A-mediated progression of ESCC, and targeting DNMT3A and HDAC9 may be a potential ESCC therapeutic strategy.
DBE-KT22: A Knowledge Tracing Dataset Based on Online Student Evaluation
Authors: Abdelrahman Ghodai , Abdelfattah Sherif , Wang Qing , Lin Yu
Published: October 2022

Online education has gained an increasing importance over the last decade for providing affordable high quality education to students worldwide. This has been further magnified during the global pandemic as more students switched to study online. The majority of online education tasks, e.g., course recommendation, exercise recommendation, or automated evaluation, depends on tracking students' knowledge progress. This is known as the Knowledge Tracing problem in the literature. Addressing this problem requires collecting student evaluation data that can reflect their knowledge evolution over time. In this paper, we propose a new knowledge tracing dataset named Database Exercises for Knowledge Tracing (DBE-KT22) that is collected from an online student exercise system in a course taught at the Australian National University in Australia. We discuss the characteristics of the DBE-KT22 dataset and contrast it with the existing datasets in the knowledge tracing literature. Our dataset is available for public access through the Australian Data Archive platform upon proper citation to the authors.
Characterization of Cellulose Nanocrystals (CNCs) Derived from Microcrystalline Cellulose (MCC) Synthesized Using Acid Hydrolysis Method.
Authors: Whba Fathyah , Mohamed Faizal , Idris Mohd Idzat , Yahya Mohd Syukri
Published: October 2022

This paper documented the synthesis of cellulose nanocrystals (CNCs) extracted from commercially-sourced microcrystalline cellulose (MCC) via acid hydrolysis and the subsequent characterization analyses of the yield. The hydrolysis process was completed using 64% sulphuric acid at 45°C for 1 hour. The yield of (CNCs) exhibited prominent physicochemical properties. The X-ray diffraction (XRD) analysis suggested that the crystallinity degree of the CNCs was 8.94% higher than MCC sample. Meanwhile, zeta potential (ζ) analysis showed that the CNCs sample possesses highly negative surface charges of -25.6±8.24 mV. Further investigation by transmission electron microscopy (TEM) analysis revealed the rod-like morphology of the CNCs sample with average diameter of 9.36±3.81nm and 83.84% crystallinity index. Whereas, via field emission scanning electron microscopy (SEM) analysis confirmed considerable reduction in particle size and changes in surface morphology post-acid hydrolysis of the CNCs, with diameter of 7.65±2.56 nm. It is envisaged that the acid hydrolysis technique is promising in synthesizing CNCs of high crystallinity with smaller size for various possible applications, potentially as a contrast agent.
Dynamical modeling and Identification of Li-ion batteries based on Squirrel Search Optimization Algorithm for Electric Vehicle Applications
Authors: DALI Ali , MESBAHI Tedjani , BARTHOLOMEÜS Patrick , Chaaban Khaled
Published: October 2022

This paper presents an advanced method for modeling and parameter identification of the lithium-ion (Li-ion) battery using an experimental characterization. A comparison study between two Li-ion models and a proposed one are performed where each model parameters are identified using three different algorithms. The obtained models are then tested and validated using experimental data obtained from a real test bench. The identification methods are implemented using a precise nonlinear model based on electric equivalent circuit of Li-ion battery and the parameter identification process formulated as a nonlinear optimization problem. In order to compare the capability of each model to represent effectively the Li-ion battery regardless of the optimization method, three optimization algorithms are involved: Squirrel Search Algorithm (SS), Particle Swarm Optimization (PSO) and Genetic Algorithm (GA). Numerical simulations along with experimental validation are performed on 20 Ah Li-ion battery. The models are compared according to the lowest fitness function levels of each algorithm and their limitation were also discussed in this paper. The obtained results show that the proposed models are able to simulate the dynamic behavior of Li-ion battery with good performances.
SPOCD1 serves as a prognostic marker in colon cancer and is associated with immune infiltration
Authors: Hui Juan , Liu Hao , An Guangzhou , Zhou Yun , Liang Junrong , He Yangsong , Wang Pei , Wang Boda , Wang Xin , Lu Yuanyuan , Zhao Xiaodi
Published: October 2022

Purpose Colon cancer is among the most common malignant tumors with poor prognosis in the world. SPOCD1 encodes transcription factor S-II family proteins whose aberrant expression is linked to tumor progression and immune regulation. However, it remains unclear whether SPOCD1 contributes to the progression and immunoregulation of colon cancer. Therefore, we sought to explore and confirm the expression of SPOCD1 in colon cancer and its association with immune regulation in this study.
Methods Based on RNA-seq data from the TCGA colon cancer project, we explored the expression of SPOCD1 and its relationship with the OS, DSS, and PFI of patients with colon cancer. Moreover, the connection between SPOCD1 and the infiltration level of immune cells, immunomodulators and chemokines and receptors in colon cancer was comprehensively investigated utilizing the TIMER, GEPIA and TISIDB databases. Finally, we confirmed the expression of SPOCD1 in colon cancer and its correlation with immune cell infiltration by immunohistochemistry and multiplex immunohistochemistry.
Results We found that SPOCD1 expression was increased in colon cancer and had tremendously diagnostic and prognostic value. The area under the curve (AUC) for SPOCD1 in COAD is 0.910 (95% CI 0.875–0.946) and high expression of SPOCD1 indicates a shorter OS, DSS and PFI in patients with colon cancer. Additionally, the increased expression of SPOCD1 in colon cancer is linked to the infiltration levels of immunomodulators, chemokines and receptors, neutrophils, macrophages and cancer-associated fibroblasts. We demonstrated that the expression of SPOCD1 was elevated in colon cancer tissues and expressed in both the nucleus and cytoplasm by immunohistochemistry. Through a multiplex immunohistochemistry experiment, we further confirmed the positive correlation between SPOCD1 expression and the expression of immune markers, including CD11b, CD163 and αSMA.
Conclusion Taken together, our results suggest that SPOCD1 can be utilized as a promising indicator for diagnosis and prognosis evaluation and has tremendous potential to modulate the immune microenvironment in the progression of colon cancer.
The impact of climate change on Quaternary glaciers of Gharaghom Basin in Iran
Authors: Azirani Tayebeh Akbari , Ghorbani Hossein
Published: October 2022

This research has mapped the quaternary equilibrium line altitude (ELA) using Whrite’s and Porter’s methods to reconstruct past and present temperatures using a regression model and to detect the potential impact of climate change on glaciers using an artificial neural network in the Gharaghom basin located in northeastern Iran. Defining a regression model between temperature and altitude based on available observation data and reconstructed data indicated that the present temperature lapse rate of -0.41°C would occur for every hundred meters of increasing altitude in stations. The reconstruction of the past temperature of the Pleistocene revealed an increase of about 10.36–10.5 ˚C for the mean annual temperature of the current temperature in the basin. The recent ELA rises from the Pleistocene 2236 m and 2200 m a.s.l according to Whrite and Porter’s methods respectively. So, considering the present temperature lapse rate and the highest altitude of 3271m in the basin, the maximum Pleistocene ELA depression would be about 800m due to modern temperature increase of 10.36–10.5°C compared to the past Pleistocene temperature. Based on the neural network prediction model, linear growth in temperature would occur for the next three decades (2021–2051) in the Gharaghom basin. Based on trend analysis, the minimum temperature has been much more affected than the maximum temperature, so global warming has caused an increase in the monthly minimum and maximum temperature in most seasons in different parts of the Basin. The average temperature rise of 0.19°C for future decades would consequently affect the water resources in the basin.
Energy System Analysis with a Focus on Future Energy Demand Projections: the case of Norway
Authors: Malka Lorenc , Bidaj Flamur , Jaku Aldona , Roçi Rexhina , Gebremedhin Alemayehu , Kuriqi Alban
Published: October 2022

Post Covid-19 pandemic and the Ukrainian war is having a significant impact on energy systems worldwide, faltering investments and threatening to throttle the expansion of primary clean energy technologies even in the case of a well-structured and managed energy system, such as Norway. This unprecedented crisis requires deeper analyses of different national energy systems. Hence, providing and highlighting needed interventions and improvements in the actual energy system in the case of Norway is crucial. The focus of this study is to analyze demand-side in households, industry, and transport sectors. LEAP model, a powerful demand-side energy system analysis tool, was used to conduct the analysis. The energy demand projections for 2050 are estimated firstly by considering a population growth rate of 0.8%. Secondly, Norway has set itself an ambitious target of decreasing GHG emissions in 2030 by 55% compared to 1990 levels and 90–95% by the year 2050. It aims the diversification of the overall national energy system. From the perspective of climate change mitigation, EVs include an attractive option, other sustainable fuel sources such as H2, biofuel mixed with diesel, the use of excess heat to cover households' heating demand supplied by industry, and integration of large-scale heat pumps driven by RES during off-peak demand is applied. Energy demand projections are uncertain, and the main goal is to show how different scenario projections up to 2050 affect the energy system of Norway, showing that the combined global warming potential (GWP) will be around 28.9 million metric CO2 from 66 million metric CO2 tones released in the current account scenario.
In-Hospital Outcomes and Biomarkers of Acute Myocardial Infarction With Concomitant Bacterial infection：A Retrospective Study
Authors: Qian Lu , Zhang Suqin , Lin Jiafeng , Li Zhuoyuan
Published: October 2022

Background
As a trigger of acute myocardial infarction (AMI), bacterial infection usually accompanies with AMI, and will lead to worse outcomes of AMI patients. We aimed to assess the prognosis of AMI with concomitant bacterial infection and find out the best laboratory examinations to recognize it.
Methods
All patients hospitalized for an AMI in cardiology department were prospectively included. Patients were stratified into those with or without concomitant bacterial infection. Outcomes and laboratory examinations were compared between groups in unadjusted and adjusted analyses.
Results
Among the 456 patients hospitalized for AMI, 120 (26%) had a concomitant diagnosis of bacterial infection. Out-comes in hospital were worse in patients with bacterial infection (more acute heart failure: 61.7% vs. 22.6%, p < 0.001, and higher all-cause mortality in hospital: 15% vs. 3.9%, p < 0.001). In the Receiver Operating Curves (ROC) of biomarkers of AMI and concomitant bacterial infection, Areas under the Receiver Operating Curves (AUC) for c-reaction protein (CRP) and CRP to Platelet Ratio (CRP/PLA) were higher than Neutrophil to Lymphocyte Ratio (NLR) and leucocyte count. (0.852(0.81–0.89) mg/L, 0.848(0.81–0.89) *10− 9mg, p < 0.001). The sensitivity of CRP and the Specificity of CRP/PLA were the highest (80% and 88%). After adjusting for confounders, CRP/PLA (> 0.08) was associated with a fivefold increased risk of bacterial infection when compared with other biomarkers (OR (95%CI) = 5.62 (2.64–11.96), p < 0.001). CRP (> 8.05) was also associated with a higher risk of bacterial infection (OR (95%CI) = 4.02 (1.81–5.85), p = 0.001).
Conclusions
Bacterial infection will lead to worse outcomes of AMI patients, including in-hospital mortality and heart failure. It is the first time to use the CRP/PLA to distinguish AMI with concomitant bacterial infection from other AMI patients. The combination of CRP > 8.05 mg/L and CRP/PLA > 0.08*10− 9mg was the best hallmark of AMI with concomitant bacterial infection.
Expression of Src kinase in hypertrophic cardiomyocytes of the left ventricle of exercising rats
Authors: Liu Mengmeng , Li Pengge , Li Lijin , Sun Mengjiao , Hou Suyun , Li Zhen , Sun Xinxin , Hua Shaohua
Published: October 2022

Objective: To identify the downstream target protein Src kinase of the SDF-1/CXCR-4 pathway in exercise rat hypertrophic cardiomyocytes.
Methods: The cardiomyocytes of 14 successfully modeled swimming rats were used as the control group, and the CXCR-4 inhibitor AMD3100 was applied to the cardiomyocytes of swimming rats for 30 min as the experimental group. The expression levels of Src kinase in the cardiomyocytes of both groups were measured using immunoblotting assay (westernblot, WB).
Results: Compared with the control group, the expression of Src kinase was significantly lower in the experimental group (P < 0.05).
Conclusion: The downstream target protein of the SDF-1/CXCR-4 pathway in exercising rat cardiomyocytes is Src kinase, which is involved in the remodeling process of the left ventricle in exercising rats.
Analysis of the differences in water chemistry characteristics and spatial-temporal variation patterns in typical areas in northern and southern Xinjiang – a case study in Fukang City and Cele County
Authors: 马 玉 , Liu Xiaolong , Bai Xiang
Published: October 2022

Water chemistry characterization of natural water bodies is important for the study of rational water resources utilization in arid zones. In this paper, the hydrochemical characteristics of natural water bodies in the south (Cele) and north (Fukang) parts of Xinjiang, China, during 2006-2016 were analysed. It was found that the ion content of surface water in Cele in South Xinjiang was higher than that in Fukang in North Xinjiang, while the opposite was true for groundwater ion content; there was a good correlation between TDS and each ion content, and the mineralisation of natural water bodies was controlled by Na+ and SO42-; the water chemistry types in Cele were Na+-Cl- and mixed, and those in Fukang were Ca2+-HCO3-, Na+-Cl- and mixed; the water bodies are mainly influenced by the control of rock weathering, with the groundwater in Fukang being controlled by evaporative crystallisation; the water bodies in Cele are mostly suitable for irrigation water, and the surface water in Fukang is more suitable for irrigation water compared to groundwater.
Silver nanoparticle toxicity on Artemia parthenogenetica nauplii hatched on axenic tryptic soy agar solid medium
Authors: Do Minh Anh , Pham Hong Lam Thi , Tran Tuyet Anh , Le Van Cam Thi , Young Tim , Le Dung Viet , Dang Hoa Thi , Doan Nhinh Thi
Published: October 2022

The use of gnobiotic brine shrimp (Artemia spp.) for ecotoxicology and bacteria-host interaction studies is common. However, requirements for axenic culture and matrix effects of seawater media can be an obstacle. Thus, we investigated the hatching ability of Artemia cysts on a novel sterile Tryptic Soy Agar (TSA) medium. We further optimized the culture conditions for temperature and salinity and assessed this culture system for toxicity screening of silver nanoparticles (AgNPs) across multiple biological endpoints. Maximal hatching of embryos occurred at 28°C and without addition of sodium chloride. When capsulated cysts were cultured on TSA solid medium Artemia were negatively impacted by AgNPs at 30–50 mgL-1 in terms of embryo hatching rate, umbrella- to nauplii-stage transformation rate, and nauplii-stage growth. At 50–100 mgL-1 AgNPs and higher, evidence of damage to lysosomal storage was recorded. At 500 mgL-1 AgNPs, development of the eye was inhibited and locomotory behavior impeded. Our study reveals that Artemia cysts can hatch on TSA solid medium which offers more controllability of axenic requirements.
High levels of m6A methylation and histone acetylation modification patterns contribute to the survival of LUAD patients
Authors: Jia LiGuo , Gao ZhenZhen , Chen Jing
Published: October 2022

Background
The m6A methylation-regulated histone acetylation modification affects the proliferation and differentiation of mouse embryonic neural stem cells, and recent studies have shown that the deacetylase SIRT1 regulates RNA m6A methylation to promote hepatocellular carcinogenesis. However, the interrelationship between the m6A methylation and histone acetylation, and the potential roles as well as interactions of related regulators in TME cell infiltration and drug sensitivity have not been explored at a holistic level.
Methods
Unsupervised clustering method was used to identify lung adenocarcinoma m6A modification patterns based on 14 m6A regulators and histone acetylation modification patterns based on 37 histone acetylation regulators. Individual samples were then quantified based on their differential gene construction models. Finally, the models were analysed in relation to transcriptional background and TME characteristics to predict potential target drugs and explore the association of m6A methylation with histone acetylation.
Result
Three histone acetylation patterns as well as two m6A methylation patterns were identified. Patients with LUAD in the low-score group had poorer overall survival times and more active cancer-related malignant pathways. m6A methylation was strongly associated with histone acetylation, and high levels of m6A methylation and histone acetylation patients had significantly superior survival and immunoreactivity. VX-680 and GW843682X may be potential drugs available for the low level m6A group.
Conclusion
This work revealed that m6A modifications and histone acetylation modifications have a non-negligible role in the development of TME diversity and complexity. We found that both m6A methylation and histone acetylation are closely associated with tumor malignant pathways. Combined m6A methylation and histone acetylation analysis will help to obtain the understanding of tumor internal regulation and provide new therapeutic directions.
Exergy and Energy Analysis for a Feasibility Trigeneration System at Kocaeli University Umuttepe Campus
Authors: KADIOĞLU Ebru KOLAY , ALTINAY Meral
Published: October 2022

In this study, a feasibility of the trigeneration system, which is one of the on-site energy production methods, was prepared for Kocaeli University Umuttepe Campus, and energy and exergy analyses were made for each point of the system and exergy destruction in the lines was found. It is aimed to be able to produce the energy, which is the most basic indicator of sustainability, at the closest point to the consumption place, thus keeping the distribution and transmission losses at a minimum level and dimensioning the designed system. The key point of the gains, losses and efficiency analyses in the lines is presented with engineering calculations based on thermodynamic proofs, exergetic efficiency and losses, the energy and balance equations of the whole system, and the feasibility of the entire trigeneration system, including mathematical modeling, energy analysis, the whole system in II. Law Efficiency, COP, Thermal efficiency were calculated. The seasonal working methods were shown by the numerical analyses prepared in the summer and winter working disciplines of the system effectively and the seasonal working methods were found and the working conditions and operating load values ​​suitable for the current season of the system were found. In addition, by performing separate mathematical models for summer and winter mode, in the light of the above-mentioned technical studies, both the reduction in the energy production costs of the country thanks to the energy that the university will produce in-house, the reduction of foreign dependency in energy demand, a feasibility report ready for investors and an academic. In this sense, it is aimed to present a ready-to-use design of a medium-sized power plant that will set an example for trigeneration studies. From the designed system; 66% exergic efficiency in winter, thermal efficiency 0.87411 with 2.05 MW thermal capacity, 63% exergic efficiency in summer, COP 0.82 and 1.5 MW cooling capacity, and 2.02 MWe instantaneous electrical power was found in summer and winter. The results have shown us that the designed system can be a facility that is open to development and worth establishing with the results obtained technically. Based on the current applications made so far, it has been determined that there are similar results between the studies in the literature and the exergy and energy analyses of the trigeneration system, which we have made feasibility in Umuttepe campus and are close to the findings obtained in the calculations in the literature.
The Novel Role of Midbody-Associated mRNAs in Regulating Abscission
Authors: Farmer Trey , Han Ke-Jun , Vaeth Katherine , Taliaferro Matthew , Prekeris Rytis
Published: October 2022

Midbodies (MBs) have been shown to function during telophase as a recruiting hub, especially for ESCRT-III complex subunits, including CHMP4B, to regulate the abscission step of cytokinesis. However, the molecular machinery governing specific protein targeting and activation at the MB remains poorly understood. Until recently, it was thought that abscission regulating proteins, such as ESCRT-III complex subunits, accumulate at the MB by directly or indirectly binding to the MB resident protein, CEP55. However, recent studies have shown that depletion of CEP55 does not fully block ESCRT-III targeting to the MB, and cells in CEP55 knock-out mice divide normally. Additionally, since MBs are microtubule-rich, proteinaceous structures, it is conceptually hard to imagine how large protein complexes, such as the ESCRT-III complex, can successfully diffuse into the MB from the cytosol in a rapid and highly regulated manner. Here, we show that MBs contain mRNAs and that these MB-associated mRNAs can be locally translated, thus, resulting in the accumulation of abscission-regulating proteins. We also demonstrate that localized MB-associated translation of CHMP4B is required for it’s targeting to the abscission site. Finally, we demonstrate that 3’-UTR-dependent CHMP4B mRNA targeting to the MB is required for successful completion of cytokinesis. Based on all this data, we propose a novel method of regulating cytokinesis and abscission by MB-associated targeting and localized translation of selective mRNAs.
An Improved Route to the Regioselective Synthesis of 1,7-dibenzyl-1,4,7,10-tetraazacyclododecane
Authors: Zhang Xiaoting , Li Huirui , Kong Dehao , Wan Fuxian
Published: October 2022

1,7-dibenzyl-1,4,7,10-tetraazacyclododecane is a crucial intermediate in the preparation of tetra-armed 1,4,7,10-tetraazacyclododecane (two benzyl groups at N1- and N7- positions and two other aromatic groups at N4- and N10- positions), 1,7-disubstituted-1,4,7,10-tetraazacyclododecane, and DO2A metal chelators, which find widespread use in medicine. Current frequently-used synthesis is costly and involves multi-steps under harsh reaction conditions, delivering low yields. We present a simpler, highly regioselective synthesis of the 1,7-dibenzyl-1,4,7,10-tetraazacyclododecane that operates under mild reaction conditions.
Aromatic plants rich in phenolic compounds as a valuable basis for dietary supplements in intestinal motility disorders
Authors: García-Giménez M.D. , Quilez-Guerrero A. , Fernandez-Arche M.A. , Puerta R. De la
Published: October 2022

Plant extracts can be an important adjuvant treatment in gastrointestinal diseases where intestinal transit is involved. Because transit disorders are often associated with infections and inflammation, in our investigation we have selected five aromatic Mediterranean plants with antimicrobial, antioxidant and anti-inflammatory activities which in certain areas are also used for culinary reasons. We evaluated the intestinal transit in mice after oral administration of hydro-alcoholic extracts of Achillea millefolium L. Sideritis angustifolia Lag., Rosmarinus officinalis L. Matricaria chamomile L., and Aloysia citriodora Palau. Total content of phenols and flavonoids and their antioxidant activity were previously determined. Rosmarinus officinalis showed the highest antioxidant capacity (p < 0.001) in the DPPH and ABTS methods with IC50 of 48.89 ± 2.98 and 27.28 ± 1.83 µg/mL respectively, in agreement with the highest phenol content. Oral administration of the extracts to mice and rats showed no signs or symptoms of toxicity in any case. The extracts of A. millefolium, R. officinalis, and M. chamomilla significantly inhibited intestinal transit (p < 0.01 and p < 0.05) when compared to the castor oil control group, with a percentage of intestinal transit similar to that of the reference antidiarrheal loperamide drug (47.8 ± 3.6%). These non-toxic plant extracts constitute a valuable basis to produce dietary supplements for intestinal motility disorders.
Ex-PRESS implantation versus trabeculectomy for long-term maintenance of low intraocular pressure in open angle glaucoma patients
Authors: Tokumo Kana , Okada Naoki , Onoe Hiromitsu , Komatsu Kaori , Masuda Shun , Okumichi Hideaki , Hirooka Kazuyuki , Asaoka Ryo , Kiuchi Yoshiaki
Published: October 2022

Purpose
To compare the efficacy of Ex-PRESS implantation (EXP) to trabeculectomy (TLE) with mitomycin C, for maintaining low target intraocular pressure (IOP) in patients with open-angle glaucoma.
Methods
Patients were randomly assigned to receive EXP or TLE. Surgical success was defined according to three target mean IOP ranges (5 mmHg ≤ IOP ≤ 18 mmHg [criterion A], 5 mmHg ≤ IOP ≤ 15 mmHg [criterion B], and 5 mmHg ≤ IOP ≤ 12 mmHg [criterion C]) representing reductions of at least 20% below baseline on two consecutive follow-up visits 3 months post-surgery, with or without antiglaucoma medication and without further glaucoma surgery.
Results
A total of 73 patients, including 30 in the EXP group and 43 in the TLE group, were included in the study. The baseline IOP was 20.4 ± 4.9 mmHg in the EXP group and 21.9 ± 7.9 mmHg in the TLE group. There were no significant differences in baseline ocular or demographic characteristics between the two groups. There was no statistical difference in IOP every 6 months. After the 3-year follow-up, success rates were A) 60.0% and 60.2%, B) 45.7% and 58.1%, and C) 31.5% and 40.5% for the EXP and TLE groups, respectively. A greater number of glaucoma medications before surgery was associated with a higher failure rate in the TLE group but not the EXP group.
Conclusions
Both procedures resulted in similar IOP reductions and success rates for low target IOP. The number of preoperative glaucoma medications was a risk factor for TLE failure.
Sex differences in the association between the effect of bariatric surgery and cancer risk: A meta-analysis including 18 million individuals
Authors: Li Ya-Dong , Huang hao , Gou Yuan-Qing , Liu Chuan
Published: October 2022

Background
Studies have suggested an association between bariatric surgery for obesity with cancer risk. Whether the effect of bariatric surgery could reduce the same excess risk of overall cancer in women and men is unknown.
Methods
PubMed, EMBASE, and Web of Science were searched to identify cohort studies up to 15 September 2022. Included studies reported the association between bariatric surgery for obesity and cancer risk in men and/or female. Random-effects meta-analyses weighted by the inverse of the variances were applied to pool sex-specific RRs and women-to-men ratios of RRs (RRRs) for overall cancer risk.
Results
18 studies involved with 18,603,812 patients (18,028,898 controls and 573,914 bariatric patients) met the inclusion criteria and were eligible for analysis. The results showed that bariatric surgery was significantly associated with decreased overall cancer risk (RR = 0.68, 95% CI 0.60, 0.78; P < 0.001) in women, but not associated with overall cancer risk (RR = 0.94, 95% CI 0.79, 1.12; P = 0.470) in men. The pooled women-to-men RRR showed an 18% lower reduction of overall cancer associated with bariatric surgery in women than in men (RRR = 0.82, 95% CI 0.75–0.90; P < 0.001). The pooled women-to-men RRR showed an 18% lower reduction of overall cancer associated with bariatric surgery in women than in men RRR = 0.82, 95% CI 0.74–0.92; P < 0.001).
Conclusion
The effect of bariatric surgery is significantly associated with a lower reduction of cancer for women compared to men.
Criterion-related validity of the International Physical Activity Questionnaire (IPAQ) in gastrointestinal cancer patients receiving outpatient chemotherapy
Authors: Iwakoshi Megumi , Tsuji Tetsuya , Takaishi Hiromasa , Hamamoto Yasuo , Sukawa Yasutaka , Hirata Kenro , Muraoka Kaori , Liu Meigen
Published: October 2022

Purpose
To investigate the criterion-related validity of the International Physical Activity Questionnaire (IPAQ) in gastrointestinal cancer patients undergoing outpatient chemotherapy.
Methods
The subjects were gastrointestinal cancer patients receiving outpatient chemotherapy. The correlations of daily activity energy expenditure (AEE) according to the IPAQ-Short-Form(IPAQ-SF), daily AEE with an activity monitor, and energy expenditure from walking were analyzed.
Results
The subjects were 50 people with a mean age of 65.6±10.5 years. The median daily AEE was 86.0 kcal with the IPAQ-SF and 374.0 kcal with the activity monitor, with no correlation between the two. The median daily energy expenditure from walking was 84.3 kcal with the IPAQ-SF and 82.3 kcal with the activity monitor, with a significant correlation (r=0.378) between the two. With a one-week chemotherapy administration interval, significant correlations were seen with daily AEE (r=0.766) and energy expenditure from walking (r=0.888), but no significant correlations were seen with intervals of two weeks or longer.
Conclusions
Using the IPAQ-SF in measurements of energy expenditure from walking during chemotherapy was found to be valid. With respect to AEE, however, when using the IPAQ-SF, it is necessary to evaluate the amount of activity for the most recent week, not a usual week.
Implications for cancer survivors
This study verified the validity of the IPAQ-SF as a screening tool to simply assess physical activity (PA) during outpatient chemotherapy. Implementation of evaluations of PA may facilitate the maintenance of daily exercise habits and assure PA, and improve the continuation of therapy and prolong survival.