bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolic fingerprinting for discrimination of DNA-authenticated Atractylodes plants using 1H NMR spectroscopy
Authors: Shirahata Tatsuya , Ishikawa Hiroshi , Kudo Teruhisa , Takada Yumiko , Hoshino Azusa , Taga Yui , Minakuchi Yusaku , Hasegawa Tomoko , Horiguchi Rina , Hirayama Takehiro , Konishi Takahiro , Takemoto Hiroaki , Sato Noriko , Aragane Masako , Oikawa Tetsuro , Odaguchi Hiroshi , Hanawa Toshihiko , Kodaira Eiichi , Fukuda Tatsuo , Kobayashi Yoshinori
Published: February 2021

Identifying different species of the genus Atractylodes which are commonly used in Chinese and Japanese traditional medicine, using chromatographic approaches can be difficult. 1H NMR metabolic profiling of DNA-authenticated, archived rhizomes of the genus Atractylodes was performed for genetic and chemical evaluation. The ITS region of the nuclear rDNA was sequenced for five species, A. japonica, A. macrocephala, A. lancea, A. chinensis, and A. koreana. Our samples had nucleotide sequences as previously reported, except that part of the A. lancea cultivated in Japan had a type 5, hybrid DNA sequence. Principal component analysis (PCA) using 1H NMR spectra of extracts with two solvent systems (CD3OD, CDCl3) was performed. When CDCl3 extracts were utilized, the chemometric analysis enabled the identification and classification of Atractylodes species according to their composition of major sesquiterpene compounds. The 1H NMR spectra using CD3OD contained confounding sugar peaks. PCA removal of these peaks gave the same result as that obtained using CDCl3 and allowed species distinction. Such chemometric methods with multivariate analysis of NMR spectra will be useful for the discrimination of plant species, without specifying the index components and quantitative analysis on multi-components.

Graphic abstract
Supporting interdisciplinary collaborative concept mapping with individual preparation phase
Authors: Tan Esther , de Weerd Jacob Gerolf , Stoyanov Slavi
Published: February 2021

Concept mapping facilitates the externalisation and internalisation of knowledge by individuals during collaborative knowledge construction. However, not much is known about the individual and collaborative learning processes during collaborative concept mapping (CCM) in interdisciplinary knowledge construction. Premised on literature on collaboration scripts to scaffold the collaboration process, this study investigates the effect of an individual preparation phase prior to collaborative work on the epistemic and social processes of knowledge co-construction, as well as the degree of interdisciplinary knowledge integration in collaborative concept mapping. A total of N = 42 third year university students were put into one of the two experimental conditions: with individual preparation phase (WIP) and without individual preparation phase (WOIP). Students worked on a collaborative assignment to integrate interdisciplinary knowledge in collaborative concept mapping. Data for analysis was derived from audio recordings of the collaborative discourse in both experimental conditions. Chi-square test was conducted to investigate if there were significant differences between the effects of WIP and WOIP on the epistemological and social dimension. Findings showed that groups in the WIP condition showed significantly more verification, clarification and positioning statements in the epistemic dimension and also significantly more integration-oriented and conflict-oriented consensus building in the social dimension as compared to groups in the WOIP condition. On the degree of interdisciplinary knowledge integration, independent sample t-tests showed that there was no significant difference for concepts, domains and cross-links between the two experimental conditions. However, there was significant difference in types of cross-links for the CCMs in the WIP condition.
The role of renewables in the Japanese power sector: implications from the EMF35 JMIP
Authors: Shiraki Hiroto , Sugiyama Masahiro , Matsuo Yuhji , Komiyama Ryoichi , Fujimori Shinichiro , Kato Etsushi , Oshiro Ken , Silva Diego Herran
Published: February 2021

The Japanese power system has unique characteristics with regard to variable renewable energies (VREs), such as higher costs, lower potentials, and less flexibility with the grid connection compared to other major greenhouse-gas-emitting countries. We analyzed the role of renewable energies (REs) in the future Japanese power sector using the results from the model intercomparison project Energy Modeling Forum (EMF) 35 Japan Model Intercomparison Project (JMIP) using varying emission reduction targets and key technological conditions across scenarios. We considered the uncertainties for future capital costs of solar photovoltaics, wind turbines, and batteries in addition to the availability of nuclear and carbon dioxide capture and storage. The results show that REs supply more than 40% of electricity in most of the technology sensitivity scenarios (median 51.0%) when assuming an 80% emission reduction in 2050. The results (excluding scenarios that assume the continuous growth of nuclear power and/or the abundant availability of domestic biomass and carbon-free hydrogen) show that the median VRE shares reach 52.2% in 2050 in the 80% emission reduction scenario. On the contrary, the availability of newly constructed nuclear power, affordable biomass, and carbon-free hydrogen can reduce dependence on VREs to less than 20%. The policy costs were much more sensitive to the capital costs and resource potential of VREs than the battery cost uncertainties. Specifically, while the doubled capital costs of VRE resulted in a 13.0% (inter-model median) increase in the policy cost, the halved capital costs of VREs reduced 8.7% (inter-model median) of the total policy cost. These results imply that lowering the capital costs of VREs would be effective in achieving a long-term emission reduction target considering the current high Japanese VRE costs.
A Review of Complement Activation in SLE
Authors: Weinstein Arthur , Alexander Roberta V. , Zack Debra J.
Published: February 2021

Purpose of Review

Complement activation is a key event in the pathogenesis of tissue inflammation and injury in systemic lupus erythematosus (SLE). This review is aimed at comparing the usefulness of measurement of complement proteins in serum/plasma (C3, C4) to complement activation (split) products in plasma and on circulating blood cells for SLE diagnosis, disease monitoring, and prognosis.

Recent Findings

Complement split products, C3dg, iC3b, and C4d, are elevated in SLE, and C3dg/C3 and iC3b/C3 ratios correlate with active SLE. C4d also is higher in patients with lupus nephritis. An elevated level of the alternative pathway split product, Bb, in early lupus pregnancy is a predictor of adverse outcomes in SLE patients with antiphospholipid antibodies. Elevated levels of cell-bound complement activation products (CB-CAPs), namely, B cell-bound C4d (BC4d) and erythrocyte-bound C4d (EC4d), within a multiparameter assay panel, may predict transition to SLE more than other lupus biomarkers. EC4d better correlates with lupus disease activity than low plasma complement levels. Elevated platelet-bound C4d (PC4d) correlates with thrombosis in SLE. Both EC4d and PC4d are increased in primary and secondary anti-phospholipid syndrome, and anti-beta2glycoproteinI antibodies may directly activate the complement system.

Abnormal levels of plasma complement split products and CB-CAPs support complement activation as an important pathogenetic mechanism in SLE and the antiphospholipid syndromes. These tests show promise for the diagnosis of SLE and monitoring of disease activity.
Application of back-propagation neural networks in the distribution of oil sand reservoirs—a case study from the western slope of the Songliao Basin, China
Authors: Sun Jing , Zhao Yuting , Yang ChangQing , Shan Xuanlong
Published: February 2021

High-quality hydrocarbon source rocks are present in the upper Cretaceous layer in the western slope of the Songliao Basin. Oil and gas have accumulated in these rocks at the shallow edge of the basin, which has led to the formation of oil sand resources. This study uses the back-propagation (BP) neural network method to predict the distribution of oil sand reservoirs and is the first study of its kind in China. First, based on the basic data collected by core sample, well log and geochemical analyses, and the reasonable selection of samples, the cores are divided into mudstone, siltstone, fine sandstone, medium sandstone, and sand, according to lithology. Second, a three-layer BP neural network model is constructed with two hidden S-type layers and one linear output layer. Third, through a comparison of the effect of different numbers of training sessions of the sample data, we demonstrate that the accuracy of the model can be increased to 90% after training the network 100,000 times. Then, the log-derived data of rocks with unknown lithologies are input into the neural network to predict whether they contain oil sands. We show that the BP neural network method can predict the distribution of oil sand reservoirs in the target horizon of the study area, and the results are consistent with research results on the corresponding sand reservoirs and sedimentary facies. Thus, we conclude that it is feasible to use the BP neural network method to predict the distribution of oil sand reservoirs.
Electron Beam Susceptibility of Enteric Viruses and Surrogate Organisms on Fruit, Seed and Spice Matrices
Authors: Butot Sophie , Galbusera Luca , Putallaz Thierry , Zuber Sophie
Published: February 2021

The objective of this study was to use high-energy electron beam (HEEB) treatments to find surrogate microorganisms for enteric viruses and to use the selected surrogates as proof of concept to investigate low-energy electron beam (LEEB) treatments for enteric virus inactivation at industrial scale on frozen blueberries. Six food matrices inoculated with HAV (hepatitis A virus), MNV S99 (murine norovirus), bacteriophages MS2 and Qβ, and Geobacillus stearothermophilus spores were treated with HEEB at 10 MeV using 4, 8 and 16 kGy doses. G. stearothermophilus spores showed the highest inactivation on all matrices except on raisins, with a dose-dependent effect. HAV reached the maximum measurable log10 reduction (> 3.2 log10) when treated at 16 kGy on raisins. MNV showed the highest resistance of all tested microorganisms, independent of the dose, except on frozen blueberries. On frozen blueberries, freeze-dried raspberries, sesame seeds and black peppercorns, HAV showed a mean inactivation level in between those of MS2 and G. stearothermophilus. Based on this, we selected both surrogate organisms as first approximation to estimate HAV inactivation on frozen blueberries during LEEB treatment at 250 keV using 16 kGy. Reductions of 3.1 and 1.3 log10 were measured for G. stearothermophilus spores and MS2, respectively, suggesting that a minimum reduction of 1.4 log10 can be expected for HAV under the same conditions.
Circulating microRNAs associated with prediabetes and geographic location in Latinos
Authors: Flowers Elena , Ramírez-Mares Juan-Daniel , Velazquez-Villafaña Marion , Rangel-Salazar Ruben , Sucher Anatol , Kanaya Alka M. , Aouizerat Bradley E. , de la Vega Monroy Maria Luisa Lazo
Published: February 2021

Background

Globally, type 2 diabetes is highly prevalent in individuals of Latino ancestry. The reasons underlying this high prevalence are not well understood, but both genetic and lifestyle factors are contributors. Circulating microRNAs are readily detectable in blood and are promising biomarkers to characterize biological responses (i.e., changes in gene expression) to lifestyle factors. Prior studies identified relationships between circulating microRNAs and risk for type 2 diabetes, but Latinos have largely been under-represented in these study samples.

Aims/hypothesis

The aim of this study was to assess for differences in expression levels of three candidate microRNAs (miR-126, miR-146, miR-15) between individuals who had prediabetes compared to normal glycemic status and between individuals who self-identified with Latino ancestry in the United States (US) and native Mexicans living in or near Leon, Mexico.

Methods

This was a cross-sectional study that included 45 Mexicans and 21 Latino participants from the US. Prediabetes was defined as fasting glucose 100–125 mg/dL or 2-h post-glucose challenge between 140 and 199 mg/dL. Expression levels of microRNAs from plasma were measured by qPCR. Linear and logistic regression models were used to assess relationships between individual microRNAs and glycemic status or geographic site.

Results

None of the three microRNAs was associated with risk for type 2 diabetes. MiR-146a and miR-15 were significantly lower in the study sample from Mexico compared to the US. There was a significant interaction between miR-146a and BMI associated with fasting blood glucose.

Conclusions/interpretation

This study did not replicate in Latinos prior observations from other racial groups of associations between miR-126, miR-146a, and miR-15 and risk for type 2 diabetes. Future studies should consider other microRNAs related to different biological pathways as possible biomarkers for type 2 diabetes in Latinos.
Supporting and leveraging science as a social activity: The value of sharing ideas early and often
Authors: Regehr Glenn
Published: February 2021

None
Applications of sum-frequency generation vibrational spectroscopy in friction interface
Authors: Liu Zhifeng , Liu Mengmeng , Zhang Caixia , Chu Hongyan , Ma Liran , Cheng Qiang , Cai Hongyun , Chen Junmin
Published: February 2021

Sum-frequency generation (SFG) vibrational spectroscopy is a second-order nonlinear optical spectroscopy technique. Owing to its interfacial selectivity, SFG vibrational spectroscopy can provide interfacial molecular information, such as molecular orientations and order, which can be obtained directly, or molecular density, which can be acquired indirectly. Interfacial molecular behaviors are considered the basic factors for determining the tribological properties of surfaces. Therefore, owing to its ability to detect the molecular behavior in buried interfaces in situ and in real time, SFG vibrational spectroscopy has become one of the most appealing technologies for characterizing mechanisms at friction interfaces. This paper briefly introduces the development of SFG vibrational spectroscopy and the essential theoretical background, focusing on its application in friction and lubrication interfaces, including film-based, complex oil-based, and water-based lubricating systems. Real-time detection using SFG promotes the nondestructive investigation of molecular structures of friction interfaces in situ with submonolayer interface sensitivity, enabling the investigation of friction mechanisms. This review provides guidance on using SFG to conduct friction analysis, thereby widening the applicability of SFG vibrational spectroscopy.
Informed-Decision Regarding Global Warming and Climate Change Among High School Students in the United Kingdom
Authors: Kurup Premnadh M. , Levinson Ralph , Li Xia
Published: February 2021

Global warming and climate change are serious issues facing humanity at present and education needs to focus on including informed-decision in classroom practices. The conceptual framework used in this study has provided interconnections that influence beliefs and understandings in providing a knowledge base for making “informed-decision” among high school students. This study was conducted in three year 9 classes in two high schools in the UK and among 65 students. An inquiry intervention model was developed using the 5E instructional model (Engage, Explore, Explain, Elaborate, and Evaluate) to identify beliefs and understanding and to strengthen students’ knowledge base. This study used a design-based research setting and utilised a mixed methodology. The Wilcoxon signed-rank tests were computed to examine the pre-post-difference among questionnaire items, and structural equation modelling (SEM) was utilised to explore the relationship between belief, understanding, and intention. Data analysis of the intervention revealed that students developed a strong understanding of the causes and effects of global warming. There is evidence that students used that knowledge to “inform-decision” in relation to global warming and climate change. Promoting informed decision-making through science teaching can encourage responsible action in the future. The real gap identified in this study is that the regular school curriculum does not engage socio-scientific issues in the real world and has no opportunity to organise an inquiry-based instructional sequence for informed decision-making.
Impact of solar heat enhanced by the use of black polypropylene sheets on the development of Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae) eggs and germinabilty of cowpea seeds
Authors: Ajayi F. A. , Peter E. , Okrikata E. , Emmanuel R. A. L. , Dattijo S. A. , Kayode E. A
Published: February 2021

Post-harvest losses caused by Callosobruchus maculatus is a major constraint to cowpea production and the improper use of synthetic insecticides by most farmers have resulted in environmental, health hazards, and development of insecticide resistance in insects. This study evaluates the efficacy of solar heat treatment using double-layered black polypropylene sheets in suppressing the development of the cowpea bruchid eggs and the effect of the heat absorbed on the germination potential of cowpea seeds. Five pairs of 2 – 3-day old adult C. maculatus were allowed to oviposit for ten days on 50 g cowpea seeds in a glass jar in the laboratory before exposure to 24, 48, 72 and 96 h solar heat treatment regimes. Infested cowpea seeds but not exposed to solarization was set along with the treatments while uninfested cowpea seeds was also set along and used as part of the germinability test. The experiment was laid out in a completely randomized design and treatments were replicated four times. The results showed that there were no significant differences among the number of eggs laid by adult C. maculatus on the cowpea seeds before solar heat treatment. Solar heat treatment of the cowpea seed at 24 h achieved 100% egg mortality of C. maculatus thus preventing adult emergence. The effect of solarization on seed viability showed that there were no significant differences between the infested cowpea seeds exposed to different solar radiation regimes when compared to uninfested cowpea seeds – range; 74% – 99% germination. The results showed that 24 h solar heat exposure of cowpea seeds in double-layered black polypropylene sheets could be the thermal death point of C. maculatus eggs on the seeds in the study area.
Profiling Chromatin Accessibility at Single-Cell Resolution
Authors: Sinha Sarthak , Satpathy Ansuman T. , Zhou Weiqiang , Ji Hongkai , Stratton Jo A. , Jaffer Arzina , Bahlis Nizar , Morrissy Sorana , Biernaskie Jeff A.
Published: February 2021

How distinct transcriptional programs are enacted to generate cellular heterogeneity and plasticity, and enable complex fate decisions are important open questions. One key regulator is the cell’s epigenome state that drives distinct transcriptional programs by regulating chromatin accessibility. Genome-wide chromatin accessibility measurements can impart insights into regulatory sequences (in)accessible to DNA-binding proteins at a single-cell resolution. This review outlines molecular methods and bioinformatic tools for capturing cell-to-cell chromatin variation using single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) in a scalable fashion. It also covers joint profiling of chromatin with transcriptome/proteome measurements, computational strategies to integrate multi-omic measurements, and predictive bioinformatic tools to infer chromatin accessibility from single-cell transcriptomic datasets. Methodological refinements that increase power for cell discovery through robust chromatin coverage and integrate measurements from multiple modalities will further expand our understanding of gene regulation during homeostasis and disease.
A Novel Frameshift Mutation in the ITGB3 Gene Leading to Glanzmann’s Thrombasthenia in a Saudi Arabian Family
Authors: Alharbi Asma , Hashmi Jamil A. , Alharby Essa , Albalawi Alia M. , Ramzan Khushnooda , Basit Sulman
Published: February 2021

Glanzmann’s thrombasthenia (GT) is an autosomal recessive congenital bleeding disorder of platelet aggregation. Mutations in ITGA2B and ITGB3 genes result in quantitative and/or qualitative abnormalities of the glycoprotein receptor complex IIb/IIIa (integrin αIIbβ3), which in turn impairs platelet aggregation and lead to GT. In this study, whole genome single nucleotide polymorphism (SNP) genotyping as well as whole exome sequencing was performed in a large family segregating GT. Analysis of the genotypes localized the disease region to chromosome 17q21.2–q21.3. Filtration of whole exome data and candidate variants prioritization identified a pathogenic variant in the ITGB3 gene. The single nucleotide deletion variant (c.2113delC) in exon 13 of the ITGB3 gene is predicted to cause a frameshift and absence of vital C-terminal domains including the transmembrane helix and the cytoplasmic domain. Clinical variability of the bleeding phenotype in affected individuals with the same mutation suggests that other genetic and nongenetic factors are responsible for determining GT features.
Foundations of Quantum Mechanics
Authors: Adlam Emily
Published: February 2021

Quantum mechanics is an extraordinarily successful scientific theory. But more than 100 years after it was first introduced, the interpretation of the theory remains controversial. This Element introduces some of the most puzzling questions at the foundations of quantum mechanics and provides an up-to-date and forward-looking survey of the most prominent ways in which physicists and philosophers of physics have attempted to resolve them. Topics covered include nonlocality, contextuality, the reality of the wavefunction and the measurement problem. The discussion is supplemented with descriptions of some of the most important mathematical results from recent work in quantum foundations, including Bell's theorem, the Kochen-Specker theorem and the PBR theorem.
Champions need an iron will: How employees use their dispositional self-control to overcome workplace incivility
Authors: De Clercq Dirk , Belausteguigoitia Imanol
Published: February 2021

This paper investigates how employees' experience of workplace incivility may steer them away from idea championing, with a special focus on the mediating role of their desire to quit their jobs and the moderating role of their dispositional self-control. Data collected from employees who work in a large retail organization reveal that an important reason that exposure to rude workplace behaviors reduces employees' propensity to champion innovative ideas is that they make concrete plans to leave. This mediating effect is mitigated when employees are equipped with high levels of self-control though. For organizations, this study accordingly pinpoints desires to seek alternative employment as a critical factor by which irritations about resource-draining incivility may escalate into a reluctance to add to organizational effectiveness through dedicated championing efforts. It also indicates how this escalation can be avoided, namely, by ensuring employees have access to pertinent personal resources.
City limits to partisan polarization in the American public
Authors: Jensen Amalie , Marble William , Scheve Kenneth , Slaughter Matthew J.
Published: February 2021

How pervasive is partisan sorting and polarization over public policies in the American public? We examine whether the barriers of partisan sorting and polarization seen in national politics extend to important local policies that shape economic development. To describe the extent of partisan sorting and polarization over local development policies, we employ conjoint survey experiments in representative surveys of eight US metropolitan areas and a hierarchical modeling strategy for studying heterogeneity across respondents. We find that strong partisans are sorted by party in some of their policy opinions, but rarely polarized. The same voters who disagree about national issues have similar preferences about local development issues suggesting a greater scope for bipartisan problem solving at the local level.
THE CONGRESS SYSTEM AND THE FRENCH INVASION OF ALGIERS, 1827–1830
Authors: DE LANGE ERIK
Published: February 2021

The Congress system that arose in Europe after the Napoleonic Wars facilitated European imperial expansionism throughout the nineteenth century. Yet, the ties between that system and expansionism have rarely been unwound and studied in detail. Taking the French invasion of Algiers in 1830 as a case in point, this article shows how the Congress system's shared discourses of security and threat perceptions as well as its common practices of concerted diplomacy fostered European imperialism in North Africa. The article emphasizes obscured continuities and understudied multilateral diplomatic efforts. It uncovers the ways in which the post-1815 system decisively shaped the aims, justifications, and execution of the French war against Algiers. European, North African, and Ottoman actors each furthered or contested the idea that the invasion was part of an international legacy dating back to the Congress of Vienna, related to the concerted repression of North African ‘Barbary piracy’. In bringing these connections to light, it becomes apparent that the post-1815 international system cannot be understood in isolation from nineteenth-century imperialism.
Psychosocial and medical predictors of 14-year mortality and morbidity in male and female coronary artery bypass graft recipients: a prospective observational study
Authors: Linden Wolfgang , Young Sandra , Ignaszewski Andrew , Campbell Tavis
Published: February 2021

Background

Psychosocial factors may influence mortality and morbidity after coronary bypass surgery (CABG), but it is unclear when, post-surgery, they best predict the outcome, if they interact, or whether results differ for men and women.

Methods

This prospective, observational study assessed depression symptoms, social support, marital status, household responsibility, functional impairment, mortality and need for further coronary procedures over 14 years of follow-up. Data were collected in-hospital post-CABG and at home 1-year later. Mortality and subsequent cardiac procedure data were extracted from a Cardiac Registry.

Results

Of 296 baseline participants, 78% (43% were women) completed data at 1-year post-CABG. Long-term survival was shorter with 1-year depression and lower household responsibility but that was not true for the measures taken at baseline [HR for depression = 1.27; 95% CI 1.02–1.59 v. 0.99 (0.78–1.25), and HR = 0.71; 95% CI 0.52–0.97 v. 0.97 (0.80–1.16)] for household responsibility. An interaction between depression symptoms and social support at year 1 [χ2 (11) = 111.05, p < 0.001] revealed a greater hazard of mortality d with increased depression only at mean (HR = 1.67; 95% CI 1.21–2.26) and high social support (HR = 2.23; 95% CI 1.46–3.40). Depression also accounted for increased event recurrence. There were no significant interactions of sex with medical long-term outcomes.

Conclusions

In a sex-balanced sample, depression and household responsibility measured at 1-year post-CABG were associated with significant variance in unadjusted and adjusted predictor models of long-term mortality whereas the same indices determined right after the procedure were not significant predictors.
Seasonal shift in epidemics of respiratory syncytial virus infection in Japan
Authors: Miyama Takeshi , Iritani Nobuhiro , Nishio Takayuki , Ukai Tomohiko , Satsuki Yuka , Miyata Hiromi , Shintani Ayumi , Hiroi Satoshi , Motomura Kazushi , Kobayashi Kazuo
Published: February 2021

In Japan, respiratory syncytial virus (RSV) infection generally has occurred during autumn and winter. However, a possible change in the seasonal trend of RSV infection has been observed recently. The current study was conducted to determine whether the epidemic season of RSV infection in Japan has indeed changed significantly. We used expectation-based Poisson scan statistics to detect periods with high weekly reported RSV cases (epidemic cluster), and the epidemic clusters were detected between September and December in the 2012–2016 seasons while those were detected between July and October in the 2017–2019 seasons. Non-linear and linear ordinary least squares regression models were built to evaluate whether there is a difference in year trend in the epidemic seasonality, and the epidemic season was shifted to earlier in the year in 2017–2019 compared to that in 2012–2016. Although the reason for the shift is unclear, this information may help in clinical practice and public health.
A waveform design method for frequency diverse array systems based on diversity linear chirp waveforms
Authors: Wang Zhonghan , Song Yaoliang
Published: February 2021

A frequency diverse array (FDA) radar has attracted wide attention, due to its capability to provide a range-angle dependent beampattern and to scan the spatial without phase shifters or rotating arrays. However, FDA systems will suffer from low echo signal energy or high sidelobe peaks when detecting targets by beamforming based on existing receivers. To reduce the sidelobe peak of detection results while increasing the echo signal energy, in this paper, we propose an FDA radar system based on diversity linear frequency modulation waveforms. Correspondingly, we propose a receiver architecture with a time-variant beamforming chain. The proposed system retains the ability of the FDA system to automatically spatial beam scanning, owing to the frequency increment across elements. By increasing the pulse duration of transmitted signals, we enhance the echo signal energy. By applying the artificial bee colony algorithm to design the bandwidth of each chirp signal, the proposed system reduces the sidelobe level of detection results while increasing pulse width. Numerical simulation results are presented to demonstrate the effectiveness of the proposed system.
High-gain and broadband SIW cavity-backed slots antenna for X-band applications
Authors: El khamlichi Dahbi , Amar Touhami Naima , Elhamadi Tajeddin , Ali Ennasar Mohammed
Published: February 2021

Substrate-integrated waveguide (SIW) technology has recently drawn attention to its benefits in the microwave field, such as integration in planar microwave circuits, low manufacturing cost, and high-quality factor compared to other technologies. In this paper, a broadband and high gain SIW cavity-backed L-shaped slot antenna structure has been designed and made for X-band applications. Three pairs of L-shaped half-wave resonators are placed on the lower wall of the cavity (backed-slots) to further expand bandwidth and improve gain. The final antenna designed operates on a band ranging from 9.4 to 10.5 GHz with a bandwidth of 11%. Moreover, the gain reaches a value of 9.5 dBi. The final antenna is realized on a Rogers RT/Duroid 5870 substrate. The gain, the reflection coefficient, and the radiation patterns are measured and compared to the EM simulation results and a very good agreement is obtained. The proposed cavity-backed L-shaped slot antenna gives a good compromise between a high gain and a large bandwidth.
CME Magnetic Structure and IMF Preconditioning Affecting SEP Transport
Authors: Palmerio Erika , Kilpua Emilia K. J. , Witasse Olivier , Barnes David , Sánchez‐Cano Beatriz , Weiss Andreas J. , Nieves‐Chinchilla Teresa , Möstl Christian , Jian Lan K. , Mierla Marilena , Zhukov Andrei N. , Guo Jingnan , Rodriguez Luciano , Lowrance Patrick J. , Isavnin Alexey , Turc Lucile , Futaana Yoshifumi , Holmström Mats
Published: February 2021

Coronal mass ejections (CMEs) and solar energetic particles (SEPs) are two phenomena that can cause severe space weather effects throughout the heliosphere. The evolution of CMEs, especially in terms of their magnetic structure, and the configuration of the interplanetary magnetic field (IMF) that influences the transport of SEPs are currently areas of active research. These two aspects are not necessarily independent of each other, especially during solar maximum when multiple eruptive events can occur close in time. Accordingly, we present the analysis of a CME that erupted on May 11, 2012 (SOL2012‐05‐11) and an SEP event following an eruption that took place on May 17, 2012 (SOL2012‐05‐17). After observing the May 11 CME using remote‐sensing data from three viewpoints, we evaluate its propagation through interplanetary space using several models. Then, we analyze in‐situ measurements from five predicted impact locations (Venus, Earth, the Spitzer Space Telescope, the Mars Science Laboratory en route to Mars, and Mars) in order to search for CME signatures. We find that all in‐situ locations detect signatures of an SEP event, which we trace back to the May 17 eruption. These findings suggest that the May 11 CME provided a direct magnetic connectivity for the efficient transport of SEPs. We discuss the space weather implications of CME evolution, regarding in particular its magnetic structure, and CME‐driven IMF preconditioning that facilitates SEP transport. Finally, this work remarks the importance of using data from multiple spacecraft, even those that do not include space weather research as their primary objective.
Sulfur bacteria promote dissolution of authigenic carbonates at marine methane seeps
Authors: Leprich Dalton J , Flood Beverly E , Schroedl Peter R , Ricci Elizabeth , Marlow Jeffery J , Girguis Peter R , Bailey Jake V
Published: February 2021

Carbonate rocks at marine methane seeps are commonly colonized by sulfur-oxidizing bacteria that co-occur with etch pits that suggest active dissolution. We show that sulfur-oxidizing bacteria are abundant on the surface of an exemplar seep carbonate collected from Del Mar East Methane Seep Field, USA. We then used bioreactors containing aragonite mineral coupons that simulate certain seep conditions to investigate plausible in situ rates of carbonate dissolution associated with sulfur-oxidizing bacteria. Bioreactors inoculated with a sulfur-oxidizing bacterial strain, Celeribacter baekdonensis LH4, growing on aragonite coupons induced dissolution rates in sulfidic, heterotrophic, and abiotic conditions of 1773.97 (±324.35), 152.81 (±123.27), and 272.99 (±249.96) μmol CaCO3 • cm−2 • yr−1, respectively. Steep gradients in pH were also measured within carbonate-attached biofilms using pH-sensitive fluorophores. Together, these results show that the production of acidic microenvironments in biofilms of sulfur-oxidizing bacteria are capable of dissolving carbonate rocks, even under well-buffered marine conditions. Our results support the hypothesis that authigenic carbonate rock dissolution driven by lithotrophic sulfur-oxidation constitutes a previously unknown carbon flux from the rock reservoir to the ocean and atmosphere.
Early identification of postpartum depression using demographic, clinical, and digital phenotyping
Authors: Hahn Lisa , Eickhoff Simon B. , Habel Ute , Stickeler Elmar , Schnakenberg Patricia , Goecke Tamme W. , Stickel Susanne , Franz Matthias , Dukart Juergen , Chechko Natalia
Published: February 2021

Postpartum depression (PPD) and adjustment disorder (AD) affect up to 25% of women after childbirth. However, there are no accurate screening tools for either disorder to identify at-risk mothers and enable them to benefit from early intervention. Combinations of anamnestic, clinical, and remote assessments were evaluated for an early and accurate identification of PPD and AD. Two cohorts of mothers giving birth were included in the study (N = 308 and N = 193). At baseline, participants underwent a detailed sociodemographic-anamnestic and clinical interview. Remote assessments were collected over 12 weeks comprising mood and stress levels as well as depression and attachment scores. At 12 weeks postpartum, an experienced clinician assigned the participants to three distinct groups: women with PPD, women with AD, and healthy controls (HC). Combinations of these assessments were assessed for an early an accurate detection of PPD and AD in the first cohort and, after pre-registration, validated in a prospective second cohort. Combinations of postnatal depression, attachment (for AD) and mood scores at week 3 achieved balanced accuracies of 93 and 79% for differentiation of PPD and AD from HC in the validation cohort and balanced accuracies of 87 and 91% in the first cohort. Differentiation between AD and PPD, with a balanced accuracy of 73% was possible at week 6 based on mood levels only with a balanced accuracy of 73% in the validation cohort and a balanced accuracy of 76% in the first cohort. Combinations of in clinic and remote self-assessments allow for early and accurate detection of PPD and AD as early as three weeks postpartum, enabling early intervention to the benefit of both mothers and children.
Withaferin A alleviates fulminant hepatitis by targeting macrophage and NLRP3
Authors: Xia Yangliu , Wang Ping , Yan Nana , Gonzalez Frank J. , Yan Tingting
Published: February 2021

Fulminant hepatitis (FH) is an incurable clinical syndrome where novel therapeutics are warranted. Withaferin A (WA), isolated from herbWithania Somnifera, is a hepatoprotective agent. Whether and how WA improves D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced FH is unknown. This study was to evaluate the hepatoprotective role and mechanism of WA in GalN/LPS-induced FH. To determine the preventive and therapeutic effects of WA, wild-type mice were dosed with WA 0.5 h before or 2 h after GalN treatment, followed by LPS 30 min later, and then killed 6 h after LPS treatment. To explore the mechanism of the protective effect, the macrophage scavenger clodronate, autophagy inhibitor 3-methyladenine, or gene knockout mouse lines NLR family pyrin domain containing 3 (Nlrp3)-null, nuclear factor-erythroid 2-related factor 2 (Nrf2)-null, liver-specific AMP-activated protein kinase (Ampk)a1knockout (Ampka1ΔHep) and liver-specific inhibitor of KB kinase β (Ikkb) knockout (IkkbΔHep) mice were subjected to GalN/LPS-induced FH. In wild-type mice, WA potently prevented GalN/LPS-induced FH and inhibited hepatic NLRP3 inflammasome activation, and upregulated NRF2 and autophagy signaling. Studies withNrf2-null,Ampka1ΔHep, andIkkbΔHepmice demonstrated that the hepatoprotective effect was independent of NRF2, hepatic AMPKα1, and IκκB. Similarly, 3-methyladenine cotreatment failed to abolish the hepatoprotective effect of WA. The hepatoprotective effect of WA against GalN/LPS-induced FH was abolished after macrophage depletion, and partially reduced inNlrp3-null mice. Consistently, WA alleviated LPS-induced inflammation partially dependent on the presence of NLRP3 in primary macrophage in vitro. Notably, WA potently and therapeutically attenuated GalN/LPS-induced hepatotoxicity. In conclusion, WA improves GalN/LPS-induced hepatotoxicity by targeting macrophage partially dependent on NLRP3 antagonism, while largely independent of NRF2 signaling, autophagy induction, and hepatic AMPKα1 and IκκB. These results support the concept of treating FH by pharmacologically targeting macrophage and suggest that WA has the potential to be repurposed for clinically treating FH as an immunoregulator.
Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis
Authors: Lou Yuqing , Xu Jianlin , Zhang Yanwei , Zhang Wei , Zhang Xueyan , Gu Ping , Zhong Hua , Wang Huimin , Lu Jun , Han Baohui
Published: February 2021

Epidermal growth factor receptor (EGFR) is a key oncogene in lung adenocarcinoma (LUAD). Resistance to EGFR tyrosine kinase inhibitors is a major obstacle for EGFR-mutant LUAD patients. Our gene chip array, quantitative polymerase chain reaction validation, and shRNA-based high-content screening identified the Akt kinase lanthionine synthetase C-like protein 2 (LANCL2) as a pro-proliferative gene in the EGFR-mutant LUAD cell line PC9. Therefore, we investigated whether LANCL2 plays a role in promoting cell proliferation and drug resistance in EGFR-mutant LUAD. In silico clinical correlation analysis using the Cancer Genome Atlas Lung Adenocarcinoma dataset revealed a positive correlation between LANCL2 and EGFR expression and an inverse relationship between LANCL2 gain-of-function and survival in LUAD patients. The EGFR-mutant LUAD cell lines PC9 and HCC827 displayed higher LANCL2 expression than the non-EGFR-mutant cell line A549. In addition, LANCL2 was downregulated following gefitinib+pemetrexed combination therapy in PC9 cells. LANCL2 knockdown reduced proliferation and enhanced apoptosis in PC9, HCC827, and A549 cells in vitro and suppressed murine PC9 xenograft tumor growth in vivo. Notably, LANCL2 overexpression rescued these effects and promoted gefitinib + pemetrexed resistance in PC9 and HCC827 cells. Pathway analysis and co-immunoprecipitation followed by mass spectrometry of differentially-expressed genes in LANCL2 knockdown cells revealed enrichment of several cancer signaling pathways. In addition, Filamin A and glutathione S-transferase Mu 3 were identified as two novel protein interactors of LANCL2. In conclusion, LANCL2 promotes tumorigenic proliferation, suppresses apoptosis, and promotes gefitinib+pemetrexed resistance in EGFR-mutant LUAD cells. Based on the positive association between LANCL2, EGFR, and downstream Akt signaling, LANCL2 may be a promising new therapeutic target for EGFR-mutant LUAD.
Increase in searches for erectile dysfunction during winter: seasonal variation evidence from Google Trends in the United States
Authors: Garijo Belén Mora , Katz Jonathan E. , Greer Aubrey , Gonzalgo Mia , López Alejandro García , Deane Leslie , Ramasamy Ranjith
Published: February 2021

Several diseases associated with erectile dysfunction (ED), such as type 2 diabetes mellitus (T2DM) and coronary artery disease (CAD), are known to have seasonal variation, with increased incidence during winter months. However, no literature exists on whether this chronological-seasonal evolution is also present within ED symptomatology. We hypothesized ED would follow the seasonal pattern of its lifestyle-influenced comorbid conditions and exhibit increased incidence during winter months. In order to investigate the seasonal variation of ED in the United States between 2009 and 2019, Internet search query data were obtained using Google Trends. Normalized search volume was determined during the winter and summer seasons for ED, other diseases known to be significantly associated with ED (T2DM and CAD), kidney stones (positive control), and prostate cancer (negative control). There were significantly more internet search queries for ED during the winter than during the summer (p = 0.001). CAD and T2DM also had significantly increased search volume during winter months compared to summer months (p < 0.001 and p = 0.011, respectively). By contrast, searches for kidney stones were significantly increased in the summer than in the winter (p < 0.001). There was no significant seasonal variation in the relative search frequency for prostate cancer (p = 0.75). In conclusion, Google Trends internet search data across a ten-year period in the United States suggested a seasonal variation in ED, which implies an increase in ED during winter. This novel finding in ED epidemiology may help increase awareness of ED’s associated lifestyle risk factors, which may facilitate early medical evaluation and treatment for those at risk of both ED and cardiovascular disease.
Molecular cannibalism: Sacrificial materials as precursors for hollow and multidomain single crystals
Authors: di Gregorio Maria Chiara , Elsousou Merna , Wen Qiang , Shimon Linda J. W. , Brumfeld Vlad , Houben Lothar , Lahav Michal , van der Boom Milko E.
Published: February 2021

The coexistence of single-crystallinity with a multidomain morphology is a paradoxical phenomenon occurring in biomineralization. Translating such feature to synthetic materials is a highly challenging process in crystal engineering. We demonstrate the formation of metallo-organic single-crystals with a unique appearance: six-connected half-rods forming a hexagonal-like tube. These uniform objects are formed from unstable, monodomain crystals. The monodomain crystals dissolve from the inner regions, while material is anisotropically added to their shell, resulting in hollow, single-crystals. Regardless of the different morphologies and growth mechanism, the crystallographic structures of the mono- and multidomain crystals are nearly identical. The chiral crystals are formed from achiral components, and belong to a rare space group (P622). Sonication of the solvents generating radical species is essential for forming the multidomain single-crystals. This process reduces the concentration of the active metal salt. Our approach offers opportunities to generate a new class of crystals.
Active methanogenesis during the melting of Marinoan snowball Earth
Authors: Zhao Zhouqiao , Shen Bing , Zhu Jian-Ming , Lang Xianguo , Wu Guangliang , Tan Decan , Pei Haoxiang , Huang Tianzheng , Ning Meng , Ma Haoran
Published: February 2021

Geological evidence indicates that the deglaciation of Marinoan snowball Earth ice age (~635 Myr ago) was associated with intense continental weathering, recovery of primary productivity, transient marine euxinia, and potentially extensive CH4 emission. It is proposed that the deglacial CH4 emissions may have provided positive feedbacks for ice melting and global warming. However, the origin of CH4 remains unclear. Here we report Ni isotopes (δ60Ni) and Yttrium-rare earth element (YREE) compositions of syndepositional pyrites from the upper most Nantuo Formation (equivalent deposits of the Marinoan glaciation), South China. The Nantuo pyrite displays anti-correlations between Ni concentration and δ60Ni, and between Ni concentration and Sm/Yb ratio, suggesting mixing between Ni in seawater and Ni from methanogens. Our study indicates active methanogenesis during the termination of Marinoan snowball Earth. This suggests that methanogenesis was fueled by methyl sulfides produced in sulfidic seawater during the deglacial recovery of marine primary productivity.
Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host–microbiota interactions
Authors: Romoli Ottavia , Schönbeck Johan Claes , Hapfelmeier Siegfried , Gendrin Mathilde
Published: February 2021

The mosquito microbiota impacts the physiology of its host and is essential for normal larval development, thereby influencing transmission of vector-borne pathogens. Germ-free mosquitoes generated with current methods show larval stunting and developmental deficits. Therefore, functional studies of the mosquito microbiota have so far mostly been limited to antibiotic treatments of emerging adults. In this study, we introduce a method to produce germ-free Aedes aegypti mosquitoes. It is based on reversible colonisation with bacteria genetically modified to allow complete decolonisation at any developmental stage. We show that, unlike germ-free mosquitoes previously produced using sterile diets, reversibly colonised mosquitoes show no developmental retardation and reach the same size as control adults. This allows us to uncouple the study of the microbiota in larvae and adults. In adults, we detect no impact of bacterial colonisation on mosquito fecundity or longevity. In larvae, data from our transcriptome analysis and diet supplementation experiments following decolonisation suggest that bacteria support larval development by contributing to folate biosynthesis and by enhancing energy storage. Our study establishes a tool to study the microbiota in insects and deepens our knowledge on the metabolic contribution of bacteria to mosquito development.