bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of mesenchymal stem cells on Treg cells in rats with colitis
Authors: Zhang Heng , Cai Wei , Xu Dan , Liu Jing , Zhao Qiu , Shao Su’E
Published: July 2023

The aim was to investigate the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSC) on dextran sulfate sodium (DSS) induced colitis in rats and its effect on regulatory T cells (Treg). A model of DSS-induced colitis was established. BM-MSC was isolated and cultured to observe the efficacy of BM-MSC on colitis, including general vital signs, weight changes, colonic length changes, colonic histopathological changes, and colonic tissue MPO activity. The expression of inflammatory factors (IFN-γ, IL-4, IL-17, TGF-β) in colonic tissues was measured by real-time PCR. The amount of CD4 + CD25 + Treg was detected by flow cytometry. Real-time PCR was used to detect Foxp3+mRNA in CD4 + CD25 + Treg, western to detect Foxp3+protein expression in CD4 + CD25 + Treg, and ELISA was used to detect IL-35 and IL-10 cytokines in CD4 + CD25 + Treg culture supernatant. Results show that intravenous injection of BM-MSC significantly improved the clinical manifestations and histopathological changes in rats with experimental DSS colitis; significantly down-regulated the expression of inflammatory factors IFN-γ, IL-4, and IL-17 and up-regulated the expression of TGF-β in colon tissues; BM-MSC also increased the number of CD4+CD25+Foxp3+Treg and enhanced the function of CD4+CD25+Foxp3+Treg in colon tissues, and up-regulated the expression of IL-35. In conclusion, BM-MSC has a certain therapeutic effect on DSS-induced colitis. It can improve the general signs of colitis rats and reduce intestinal injury and inflammatory response. The immunoregulatory effect of BM-MSC is achieved by enhancing the function of CD4+CD25+Foxp3+Treg and up-regulating the secretion of immunosuppressive inflammatory factors.
Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats
Authors: Li Hangqi , Zhou Qiuying , Chen Yanlu , Hu Huijie , Gao Liang , Takahata Toru
Published: July 2023

We previously revealed the presence of ocular dominance columns (ODCs) in the primary visual cortex (V1) of pigmented rats. On the other hand, previous studies have shown that the ipsilateral-eye domains of the dorsal lateral geniculate nucleus (dLGN) are segregated into a handful of patches in pigmented rats. To investigate the three-dimensional (3D) topography of the eye-specific patches of the dLGN and its relationship with ODCs, we injected different tracers into the right and left eyes and examined strain difference, development, and plasticity of the patches. Furthermore, we applied the tissue clearing technique to reveal the 3D morphology of the LGN and were able to observe entire retinotopic map of the rat dLGN at a certain angle. Our results show that the ipsilateral domains of the dLGN appear mesh-like at any angle and are developed at around time of eye-opening. Their development was moderately affected by abnormal visual experience, but the patch formation was not disrupted. In albino Wistar rats, ipsilateral patches were observed in the dLGN, but they were much fewer, especially near the central visual field. These results provide insights into how ipsilateral patches of the dLGN arise, and how the geniculo-cortical arrangement is different between rodents and primates.
Relating white matter microstructure in theoretically defined addiction networks to relapse in alcohol use disorder
Authors: Roediger Donovan J , Griffin Claire , Marin Frances V , Verdoorn Hannah , Fiecas Mark , Mueller Bryon A , Lim Kelvin O , Camchong Jazmin
Published: July 2023

Theoretical models group maladaptive behaviors in addiction into neurocognitive domains such as incentive salience (IS), negative emotionality (NE), and executive functioning (EF). Alterations in these domains lead to relapse in alcohol use disorder (AUD). We examine whether microstructural measures in the white matter pathways supporting these domains are associated with relapse in AUD. Diffusion kurtosis imaging data were collected from 53 individuals with AUD during early abstinence. We used probabilistic tractography to delineate the fornix (IS), uncinate fasciculus (NE), and anterior thalamic radiation (EF) in each participant and extracted mean fractional anisotropy (FA) and kurtosis fractional anisotropy (KFA) within each tract. Binary (abstained vs. relapsed) and continuous (number of days abstinent) relapse measures were collected over a 4-month period. Across tracts, anisotropy measures were typically (i) lower in those that relapsed during the follow-up period and (ii) positively associated with the duration of sustained abstinence during the follow-up period. However, only KFA in the right fornix reached significance in our sample. The association between microstructural measures in these fiber tracts and treatment outcome in a small sample highlights the potential utility of the three-factor model of addiction and the role of white matter alterations in AUD.
Evaluation of the default mode network using nonnegative matrix factorization in patients with cognitive impairment induced by occupational aluminum exposure
Authors: Zhang Feifei , Liu Bo , Shao Yinbo , Tan Yan , Niu Qiao , Wang Xiaochun , Zhang Hui
Published: July 2023

Aluminum (Al) is an important environmental pathogenic factor for neurodegenerative diseases, especially mild cognitive impairment (MCI). The aim of this study was to evaluate the gray matter volume of structural covariance network alterations in patients with Al-induced MCI. Male subjects who had been exposed to Al for >10 years were included in the present study. The plasma Al concentration, Montreal cognitive assessment (MoCA) score, and verbal memory assessed by the Rey auditory verbal learning test (AVLT) score were collected from each participant. Nonnegative matrix factorization was used to identify the structural covariance network. The neural structural basis for patients with Al-induced MCI was investigated using correlation analysis and group comparison. Plasma Al concentration was inversely related to MoCA scores, particularly AVLT scores. In patients with Al-induced MCI, the gray matter volume of the default mode network (DMN) was considerably lower than that in controls. Positive correlations were discovered between the DMN and MoCA scores as well as between the DMN and AVLT scores. In sum, long-term occupational Al exposure has a negative impact on cognition, primarily by affecting delayed recognition. The reduced gray matter volume of the DMN may be the neural mechanism of Al-induced MCI.
Shedding a light on sex disparity in clinical trials in CKD patients
Authors: Mallamaci Francesca , Tripepi Giovanni
Published: July 2023

Clinical studies do not include an adequate proportion of female participants, and research data on drug efficacy and safety are generally collected from studies including a majority of men and extrapolated to women. This article describes the imbalance of male and female distribution in clinical studies, including patients with chronic kidney disease. The lack of sex equity in clinical research is a real ‘public health problem’ because not reporting sex-specific results may result in the loss of information on how a drug works according to sex. Therefore, it is essential to plan more research in the field of sex disparities in clinical studies to identify why women are underrepresented and to promote initiatives to expand women’s participation in clinical studies.
Quantification of chlamydiae in the endangered Houston toad (Anaxyrus houstonensis)
Authors: Villamizar Andrea , Guerra Trina , Tocidlowski Maryanne E , Forstner Michael R J , Hahn Dittmar
Published: July 2023

Two primer set/probe combinations targeting variable regions on the 23S rRNA gene were designed to detect and quantify chlamydiae in DNA extracted from brain swabs of the endangered Houston toad (Anaxyrus houstonensis) using SYBRGreen- and Taqman-based quantitative polymerase chain reaction (qPCR). Prevalence and abundance values for samples were generally different between SYBRGreen- and Taqman-based detection methods, with higher specificity observed for Taqman-based detection. Of the 314 samples analyzed, initial screening with SYBRGreen-based qPCR retrieved 138 positive samples, of which 52 were confirmed by Taqman-based analyses as chlamydiae. All of these samples were subsequently identified as Chlamydia pneumoniae by specific qPCR and confirmed by comparative sequence analyses of 23S rRNA gene amplicons. These results demonstrate the usefulness of our developed qPCR methods to screen for and verify prevalence of chlamydiae in DNA of brain swabs, and ultimately specifically identify and quantify chlamydiae, specifically C. pneumoniae in these samples.
Multidrug-resistant phenotypes among biofilm-producing staphylococcal isolates from farm-raised fish: a molecular scrutiny
Authors: Hamza Muneeb , Sivaraman Gopalan Krishnan , Mothadaka Mukteswar Prasad
Published: July 2023

Aims

To describe the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from farm-raised fish.

Methods and results

Fish samples (n = 180) collected from aquaculture farms in three major districts of Kerala, yielded 45 methicillin-resistant staphylococci (MRS) (25%) isolates. All of the isolates were resistant to tested beta-lactams, and 19 (42.22%) were resistant to flouroquinolones. According to the resistance profile of each isolate, 33 of 45 (73.33%) are identified as multidrug-resistant (MDR) isolates, with an average multiple antibiotic resistance (MAR) index of 0.41 ± 0.09. Notably, virulence determinants such as classical enterotoxin (sea & seb), and SE-like toxin (seg & sei) genes co-existed with Antimicrobial Resistance Genes (ARGs) in isolates studied. Regardless of the isolates being strong biofilm producers, only 23 (51.1%) harbored icaA and icaD genes. The heterogeneity of MRSA (n = 17) clones were evident as the species belonged to three major spa types (t345, t2526, and t1309) and sequence types (STs), viz., ST772, ST88, and ST672.

Conclusion

The current study on molecular characterization of the MRS isolates sheds light on the preventive measures that need to be implemented to limit the spread of AMR pathogens in aquaculture.
Novel resources of chitinolytic bacteria isolated from Yok Don National Park, Vietnam
Authors: Tran Dinh Minh , Huynh To Uyen , Nguyen Thi Huyen , Do Tu Oanh , Nguyen Quang Vinh , Nguyen Anh Dzung
Published: July 2023

Aims

This study focused on the isolation and characterization of chitinolytic bacteria from Yok Don National Park, Vietnam for future studies regarding biofertilizers and biocontrol agents.

Methods and results

Chitinolytic bacteria were isolated from soils and chitin flakes soaked in river water at the National Park. On the basis of the halo zones caused by colloidal chitin degradation and colony morphologies, 12 chitinolytic strains were chosen from 15 700 isolates for various examinations. Findings from 16S rDNA analysis indicated that among these strains, 10 could be identified as different species, and the remaining 2 showed less identity to known species and genera. The 12 bacteria possess numerous properties concerning plant growth promotion and/or phytopathogenic biocontrol. Paenibacillus chitinolyticus YSY-3.1, which exhibited the highest chitinase activity and remarkable properties for plant growth, was chosen for sequencing and draft genome analysis. The results showed that the genome is 6571 781 bp in length with 6194 coding sequences, 52.2% G + C, and 96.53% ANI value. It harbors the chitinolytic system comprising 22 enzymes. Among these enzymes, PcChiQ has a loop structure different from that of known family 19 chitinases, PcChiA contains two GH18 catalytic domains rarely found in microorganisms, and PcChiF contains three GH18 catalytic domains that have never been reported.

Conclusions

The 12 identified chitinolytic bacteria exhibit great potential for further studies on plant growth-promoting and/or biocontrol properties. Among these bacteria, two strains might be good candidates for next examinations concerning novel species and/or genera, and strain YSY-3.1 could possess a novel chitinolytic system.
Cancer survivors’ health behaviors and outcomes: a population-based study of sexual and gender minorities
Authors: Boehmer Ulrike , Chang Shine , Sanchez Nelson F , Jesdale Bill M , Schabath Matthew B
Published: July 2023

Background

Most case-control studies compare cancer survivors with general population controls without considering sexual orientation or gender identity. This case-control analysis compared health risk behaviors and health outcomes among sexual and gender minority cancer survivors to those of matched sexual and gender minority participants without cancer (controls).

Methods

Using data from the 2014-2021 Behavioral Risk Factor Surveillance System, a population-based sample of 4507 cancer survivors who self-identified as transgender, gay men, bisexual men, lesbian women, or bisexual women were 1:1 propensity score matched, using age at survey, race and ethnicity, marital status, education, access to health care, and US census region. Within each sexual and gender minority group, behaviors and outcomes were compared between survivors and participants without cancer, and survivors’ odds ratios and 95% confidence intervals calculated.

Results

Gay male survivors had higher odds of depression, poor mental health, limited usual activities, difficulty concentrating, and fair or poor health. Few differences were observed between bisexual male survivors and participants without cancer. Compared with controls, lesbian female survivors had greater odds of overweight–obese status, depression, poor physical health, and fair or poor health. Bisexual female survivors had the highest rates of current smoking, depression, poor mental health, and difficulty concentrating across all sexual and gender minority groups. Statistically significantly different from transgender controls, transgender survivors had greater odds of heavy alcohol use, physical inactivity, and fair or poor health.

Conclusions

This analysis revealed an urgent need to address the high prevalence of engaging in multiple health risk behaviors and not following guidelines to avoid second cancers, additional adverse outcomes, and cancer recurrences among sexual and gender minority cancer survivors.
Comparing Cognitive Disengagement Syndrome Growth in Youth With and Without Spina Bifida
Authors: Darow Eva L , Flax Marcus A , Clark Olivia E , Holmbeck Grayson N , Smith Zoe R
Published: July 2023

Objective

Cognitive disengagement syndrome (CDS; formally known as sluggish cognitive tempo), difficulties with social engagement, and lower levels of autonomy have been identified as maladaptive comorbidities in youth with spina bifida (SB). This study compared growth curves of CDS for youth with and without SB and examined whether these trajectories were associated with later functioning.

Methods

Longitudinal data spanning 8 years included youth with SB (n = 68, Mage = 8.34) and a demographically matched sample of typically developing (TD) peers (n = 68, Mage = 8.49). Adolescents, along with their caregivers and teachers, reported on youth social skills, behavioral functioning, and CDS. Growth curve models were examined by comparing CDS trajectories by SB status.

Results

Growth curves indicated that youth with SB had higher levels of teacher-reported CDS at ages 8 and 9, but growth curves were relatively stable for both groups. When predicting social skills, higher levels of teacher-reported (but not mother-reported) CDS at baseline predicted worse social functioning for both youth with and without SB in adolescence. For the slope findings, higher rates of mother-reported CDS over time predicted worse social skills (β = −0.43) and lower levels of youth decision-making (β = −0.43) for the SB group, while higher rates of teacher-reported CDS predicted worse social skills for the TD group.

Conclusion

Next steps include understanding the impact that impaired social functioning and restricted autonomy have on youth with and without SB due to CDS to inform interventions. Additionally, advocacy for increased awareness of CDS-related impairment is needed, particularly for youth with chronic health conditions.
(219) AUTOLOGOUS PLATELET-RICH PLASMA IMPROVES ENDOTHELIAL AND TADALAFIL-INDUCED RELAXATIONS IN CORPUS CAVERNOSUM FROM PATIENTS WITH ERECTILE DYSFUNCTION
Authors: Javier Angulo , Dr. Esaú Fernández-Pascual , Dr. Raphael Curvo , Dr. Argentina Fernández , Luis Bueno José , Ignacio Martínez-Salamanca Juan ,   Funding: Ministry of Economy and Competitiveness and co-financed by FEDER funds, Instituto de Salud Carlos III, PI17/02001, Spanish Government
Published: July 2023

Objectives

Available clinical evidence suggests the potential therapeutic use of intracavernosal injections of platelet-rich plasma (PRP) to treat erectile dysfunction (ED). However, the effects of PRP on human cavernosal function have not been elucidated. Our aim was to evaluate the effects of acute exposure to autologous PRP on relaxant capacity of human corpus cavernosum (HCC) from patients with ED.

Methods

HCC strips were obtained from four ED patients undergoing penile prosthesis insertion and giving informed consent. Previous to intervention, PRP and platelet-poor plasma (PPP) were obtained from patients by apheresis and stored at -20°C until functional procedures. Endothelial relaxation to acetylcholine (ACh) was evaluated before and after 60 min exposure to PRP or PPP (1% v/v) in organ chamber. Relaxant response to the PDE5 inhibitor, tadalafil, was also evaluated in PRP or PPP (1%) treated HCC from the same patient.

Results

Average age of patients was 67-years-old (range 61-71) while hypertension and smoking history was present in 3/4, diabetes in 2/4, and obesity in 1/4. Mean platelet content in PRP was 1,096x10 9 /L (range 786 to 1,284 x109/L) while negligible platelet content was obtained in any PPP. Exposure to autologous PRP but not PPP significantly enhanced (p<0.0001 by two-factors ANOVA) ACh-induced endothelial relaxations (pEC50 5.75±0.65 vs. 7.16±0.50; Emax 57.3±14.8% vs. 88.5±2.0%; before and after 1%PRP, respectively). Additionally, HCC treated with autologous PRP displayed augmented relaxant responses to tadalafil (p=0.0018 by two-factors ANOVA) with respect to those treated with PPP (pEC50 6.08±0.5 vs. 6.82±0.51 for PPP and PRP, respectively).

Conclusions

Acute exposure to autologous PRP improves endothelial function and functional response to PDE5 inhibition. These results support the reported clinical improvement of erectile function in patients with ED and suggest that PRP injection might induce a functional effect on cavernosal tissue in addition to the claimed restorative/regenerative effect.

Conflicts of Interest

None
(47) MAJORITY OF ERECTILE DYSFUNCTION PATIENTS WOULD HAVE PREFERRED EARLIER IMPLANTATION OF THEIR PENILE PROSTHESIS: VALIDATION OF THE RECENTLY CHANGED EAU GUIDELINES
Authors: Dr. Andries Van Huele , Dr. Jonas Mennes , Prof. Eric Chung , Prof. Koenraad Van Renterghem ,   Accepted in the “International Journal of Impotence Research - Nature” with the same title
Published: July 2023

Objectives

Until 2019, the European Association of Urology (EAU) guidelines recommended the implantation of a penile prosthesis as a third-line therapy, which has since then changed to "if other treatments fail or depending on the patient's preference", thus a more patient oriented approach. The primary endpoint of this study was to assess whether patients with a penile prosthesis and their partner would have preferred earlier implantation. Secondary endpoints were how much earlier and why patient and partner wanted earlier implantation, satisfaction of patient and partner and if patient and partner would have recommended the intervention.

Methods

We selected patients operated between April 2013 and December 2019. Inclusion criteria were a virgin implantation of an inflatable penile prosthesis (IPP) and a signed informed consent with agreement for a telephonic interview. Exclusion criteria were combined implants and follow-up less than one year. This resulted in a series of 155 patients and 65 partners. Telephonic interview was conducted in a structured manner. Further data were collected retrospectively.

Results

The preference for earlier implantation was reported in 59.4% of patients and 46.2% of partners. Of them, respectively 53.2% and 46.6% would have wanted the prosthesis more than five years earlier. Satisfaction was seen in 83.2% of patients and 73.8% of partners. Respectively 82.6% and 78.4% of patients and partners would recommend the procedure.

Conclusions

The majority of erectile dysfunction patients would have preferred earlier implantation of their IPP. Our results indicate that a more patient-oriented approach with good counseling is desirable and that the “three-level” concept must be abandoned.

Conflicts of Interest

The authors declare no competing interests.
(46) VASECTOMY: PREVALENCE AND SEX LIFE
Authors: Prof. Kathleen Herkommer , Matthias Jahnen , Anna Rechberger , Stefan Schiele , Dr. Christian Leiber-Caspers , Helga Schulwitz , Prof. Juergen E Gschwend
Published: July 2023

Objectives

Although vasectomy is a very safe and effective contraceptive procedure with few side effects, men often fear that this procedure could have a negative impact on their sex life. The aim of this study was to assess the prevalence of vasectomy among middle-aged men in Germany and to investigate possible associations between a previous vasectomy and factors of sex life.

Methods

Data on sociodemographics, sexual activity, and satisfaction with sex life from heterosexual men of the Bavarian Men's Health-Study were analyzed. Validated questionnaires, such as the International Index of Erectile Function (IIEF-6), the Erection-Hardness-Score and the Sexual-Complaints-Screener for Men were used to record the presence of the following sexual dysfunctions: Erectile dysfunction, premature ejaculation, and low libido.

Results

The analysis included 2,330 men with a mean age of 50.4 ± 0.8 years. Vasectomy was performed in 12.6% (n=294) on average 9.2 ± 5.8 years ago. 88.2% had a steady partner (with vasectomy 95.9%, without vasectomy 87.1%; p<0.001) and 73.3% had children (with vasectomy 86.7%, without vasectomy 71.4%; p<0.001). 80.8% were satisfied with their sex life (with vasectomy 85.6%, without vasectomy 80.1%; p=0.027).

Erectile dysfunction was recorded in 13.5% of vasectomized men (without vasectomy 20.5%; p=0.005), premature ejaculation in 6.7% (without vasectomy 5.1%; p=0.289) and low libido in 4.8% (without vasectomy: 7.0%; p=0.159). In multiple logistic regression vasectomy was associated with a lower risk of erectile function (OR 0.61; 95%CI 0.42-0.89), there was no association with premature ejaculation (OR 1.49; 95%CI 0.86-2.59) and low libido (OR 0.66; 95%CI 0.36-1.18) respectively.

Conclusions

The results of this study can allay patients’ fears about vasectomy. Vasectomy represents a safe contraceptive method with no evidence of negative impact on men's sexual function, sexual frequency or satisfaction with sex life.

Conflicts of Interest

none
(51) DO WOMEN WISH TO TALK ABOUT SEX?
Authors: Joanna Ghigo
Published: July 2023

Objectives

Our study aims at establishing the prevalence of sexual dysfunction in women attending gynecology clinics, and explore women’s preferences about the routine introduction of sexual function questions in general gynecological consultations.

Methods

120 Caucasian women referred to gynaecology outpatients were identified and invited to complete an anonymous questionnaire. Written consent was sought.

Questions about frequency of sexual activity, dysparunia, problems with desire, arousal, orgasm and affect on Quality of Life were included. Participants were asked whether they agreed to the gynaecologist asking questions related to sexual function during their consultation, and whether they felt comfortable with their answers being recorded in their hospital notes.

Results

108 participants completed the questionnaires. Respondents were divided in 2 groups: a premenopausal group aged less than 50 years - 62 patients (57%) and a postmenopausal group aged over 50 years -46 (43%).

55 (89%) premenopausal patients reported regular sexual activity, compared to 32 (69%) postmenopausal patients. 26 (42%) premenopausal patients reported sexual dysfunction, with pain (65%) and problems with orgasm (33%) being most frequent whilst postmenopausal patients complained of vaginal dryness (85%), pain (70%) and difficulty in arousal (68%).

70 % of all respondents were favourable to the gynaecologist asking sexual function questions. A statistically significant difference between the two groups was noted.(p=0.033). Only 25 (23%) respondents agreed to this information be kept in their hospital notes.

Overall, women were more likely to discuss sexual dysfunction if they were affected. Being asked questions by the gynaecologist made women feel that it ‘normalized the issue.’ Postmenopausal women expressed some reluctance; possibly due to their decreased interest in sexual activity but also their more conservative values.

Conclusions

Our study confirms that women would like to be asked sexual dysfunction questions during their gynaecological consultation in a sensitive manner.

Conflicts of Interest

I have no conflict of interest.
(94) A COGNITIVE BEHAVIORAL INTERVENTION FOR FEMALE SEXUAL AROUSAL DISORDERS USING A VIBRATING DEVICE
Authors: Dr. Jesús E Rodríguez , Lucrecia Martínez , Natalia Bonachera
Published: July 2023

Objectives

We will investigate the safety and preliminary evidence of the effectiveness of a new Cognitive Behavioral Therapy (CBT) in combination with a vibrating device for the treatment of Female Sexual Arousal Disorders (FSAD).

Methods

A one-group pretest-posttest multicenter design was used. Main outcome measure was assessed using the Female Sexual Function Index (FSFI). A total of 16 patients aged between 19 and 48 years of age (mean = 28.12 years, standard deviation (SD) = 1.92) met criteria and were instructed in the use of a small, portable vibration device over 12 weeks. The inclusion criteria were: Premenopausal women between 18 and 50 years of age who were diagnosed with FSAD and were in a stable monogamous relationship with a male or female partner. Exclusion criteria were: If they were pregnant or nursing or were diagnosed with or being treated for depression, anxiety, psychosis, bipolar disorder, or substance abuse within 6 months before screening. The CBT consists of three educational sessions to provide patients with greater knowledge, awareness, and control of the excitation reflex using a bendable vibration prior to sexual intercourse called Crescendo®.

Results

There was a significant improvement score in domain of FSFI Arousal (mean pre = 1.31 (SD) = 0.41, mean post = 2.27 (SD) = 0.55, Wilcoxon test pre vs. post p-value = 0.02) and Lubrication (mean pre = 1.23 (SD) = 0.41, mean post = 2.38 (SD) = 0.60, Wilcoxon test pre vs. post p-value = 0.02).

Conclusions

In this study, CBT in combination with a vibrating device is a potential treatment for sexual arousal difficulties. It was associated with a significantly increased from baseline in Arousal and Lubrication domain score of the FSFI, with no adverse effects related to the treatment. Future studies with, larger group of patients, with larger follow-up and group control are needed.

Conflicts of Interest

The authors declare that they have no known competing financial interests.
(44) SHAEER'S CAVERNOTOME: THE FIRST CORING / GRINDING CAVERNOTOME
Authors: Prof. Osama Shaeer , Prof. Kamal Shaeer
Published: July 2023

Objectives

Fibrosis of the corpora cavernosa follows removal of an infected implant or protracted / recurrent priapism. Penile prosthesis implantation in cases with fibrosis is both difficult and risky. Complications include perforation and/or failure. Shaeer’s Cavernotome (patent application number PCT/EG2021/050003) is a coring / grinding cavernotome that relies on rotational movement without force instead of forward stabbing notorious for complications.

Methods

Eighteen patients with severe corporal scarring were operated upon. Surgery is performed through a peno-scrotal incision with urethral catheter in place. Two cm long core of fibrous tissue is excised with scalpel, excavating the corporotomies. Shaeer’s Cavernotome (ShC) is lodged-in against the fibrous tissue. Rotational coring is performed proximally and distally with the penis on stretch. ShC doubles as a sizer. Following coring, penile prosthesis implantation proceeds.

Results

Dilation of the corpora cavernosa was successful up to either girth 11 or 13 Hegar with a single pass, in 17 out of 18 patients. Average coring time was 8 minutes ±3.2. No perforations or infections were encountered

Conclusions

Shaeer’s Cavernotome facilitates excavation of scarred corporal bodies without forward stabbing, thereby decreasing the difficulty and risk.

Conflicts of Interest

Author is the inventor of the instrument.
(93) SURGICAL OUTCOMES OF URETHRAL LENGTHENING IN THE CONTEXT OF GENITAL GENDER-CONFIRMING SURGERY IN TRANSGENDER MEN
Authors: Marco Falcone , Mirko Preto , Ilaria Ferro , Cirigliano Lorenzo , Peretti Federica , Plamadeala Natalia , Ceruti Carlo , Gontero Paolo
Published: July 2023

Objectives

Our study aim is to analyze the surgical and functional outcomes after urethral reconstruction in consecutive series of transgender men who underwent genital gender-confirming surgery.

Methods

A retrospective monocentric cohort study was conducted. From 2015 to 2022, patients who underwent urethral lengthening in the context of GGCS were enrolled.

Surgical outcomes and postoperative complications were reported. Three questionnaires were administered to evaluate patients’ urinary function (CCO-EPIC, ICIQ-SF) and patients’ psychological well-being (BDI - scale of depression). A 6-item ad hoc created questionnaire was administered to inquire patients’ reported outcomes.

Results

31 patients were included in the study. 11 underwent metoidioplasty (Group A), while 20 underwent phalloplasty with different techniques (Group B). The descriptive characteristics between the two groups were comparable, except for the BMI resulting greater in Group B (p-value 0.012). Average follow-up was 18 months (±18SD). Mean operative time was 160 minutes (±43 SD). No significant intraoperative complications occurred. Early postoperative complication rate resulted significantly higher for group B (83.3% p-value 0.034). The most frequent complication, urethral fistula, healed in all cases after prolonged catheterization. Overall 9.6% of patients needed a surgical revision to address urethral complications. CCO-EPIC and ICIQ-SF administered preoperatively and 1-year after surgery showed worsening in urinary function (p-value = 0.04 and p-value = 0.02 respectively), BDI score showed a significant improvement in postoperative settings (p-value= 0.02). 82 % of the patients after 1-year follow-up were satisfied with the procedure and 81 % declared that surgery had a positive impact on their quality of life. The main limitations of the study are the limited follow-up, the non-randomized nature of the study and the small number of patients.

Conclusions

Urethral reconstruction in the context of GGCS is a challenging reconstructive surgery burdened by high incidence of postoperative complications. Despite that, it may contribute to improve the psychological well-being of transgender men.

Conflicts of Interest

This study is free from commercial influence or bias and the authors had no conflict of interest.
(243) PERCEPTION OF HEALTHCARE FOR TRANSGENDER PEOPLE IN CROATIA
Authors: Iva Zegura , Priv-Doz. Andrea Jambrosic Sakoman , Karlo Dalić
Published: July 2023

Objectives

No systemic care under specific gender clinics for transgender people exists in Croatia. There is a National list of experts in the health care of transgender people which did not expand since its establishment in 2015 with new competent professionals. The main focus of the study was to find out how experts and NGOs perceive the existing level of healthcare for transgender people.

Methods

The online study is conducted in 2021 on a sample of experts from the National list of experts in healthcare for transgender people and two main NGOs that are dealing with transgender rights. The results are analyzed mainly using the qualitative methodology and to a lesser extent quantitative methodology. The thematic analysis was performed by two independent researchers.

Results

The analysis has shown three main themes: 1) Positive aspects of healthcare for transgender people; 2) Negative aspects of healthcare for transgender people; 3) Improvements in the healthcare of transgender people. Perceived positive aspects are: the existence of care options for trans people within the health care system; achieved progress in the implementation of Standards of care; availability of information regarding health care. Perceived negative aspects are a lack of competent professionals; geographically centralized health care; experts working in different hospitals; no systematic education for new professionals. Proposed improvements relate to establishing a professional association of experts for transgender health, education of new experts, decentralization of health care, and standardization of procedures of assessment and treatment within each profession.

Conclusions

Obligatory systematic education regarding the health care of trans people is necessary on graduate and postgraduate levels as well as establishing standardized procedures in diagnostic assessment of trans people for each profession included in interdisciplinary teams. A new round of discussion between professionals, transgender NGOs, and stakeholders should be made in order to remove existing barriers to complete and affirmative health care for transgender people in Croatia.

Conflicts of Interest

The authors declare no conflict of interest.
(187) MAX AND AVERAGE FLOW RATE ARE CORRELATED WITH SEVERITY OF ERECTILE DYSFUNCTION
Authors: Dr. Mohamad Habous , Dr. Simone Giona , Prof. Saleh Binsaleh , Prof. Anthony Mundy
Published: July 2023

Objectives

Numerous cross-sectional epidemiological studies have reported a clear and clinically significant association between lower urinary tract symptoms (LUTSs) and erectile dysfunction (ED) in aging men worldwide.

We aimed to investigate the severity of ED symptoms with flow rate specifically.

Methods

All patients came to our urology clinic, complaining of LUTSs for at least 3 months were prospectively included in this study, in one year. Complete medical history including international index of erectile function (IIEF-5) and international prostate symptom score (IPSS) questionnaires, physical examination and comorbidity profile were collected. Patients were stratified according to IIEF-5 score into 4 groups: no or mild ED, mild to moderate ED, moderate ED and sever ED. Standard uroflowmetry test was done, and prostate size was calculated through transrectal ultrasound (TRUS). Patients with middle lobe, urethral stricture and previous lower urinary tract surgery were excluded from the study. Database was done with Microsoft Excel and statistical analysis was done by SPSS software.

Results

166 patients had complete data and were included in this analysis. The mean age was 57,and 26% were diabetic. When running Kruskal-Wallis Test it, showed that there is a statistical difference between the groups, the lower is the IIEF score (sever ED), the lower is the max flow (P<0.049) and the average flow (P<0.025). There was an inverse correlation between max flow rate and Prostate volume, the bigger is the prostate the lower tend to be the max flow (p<0.000), and same for average flow (P<0.001). There was a slight but significant (P<0.006) increase in severity of ED with the increase in prostate volume. The older were the patients the worse is the ED and the worse the flow rate (p<0.000). The flow rate was nor correlated neither with dyslipidemia nor with BMI

Conclusions

Flow rate is correlated with severity of ED symptoms and prostate volume.

Conflicts of Interest

None
(245) FLACCID PENILE COLOUR-DOPPLER ULTRASOUND REPRESENTS A BETTER MARKER OF SYSTEMIC CARDIOVASCULAR STATUS THAN CAROTID COLOUR-DOPPLER ULTRASOUND
Authors: Prof. Francesco Lotti , Dr. Sarah Cipriani , Dr. Beatrice Gasperetti , Dr. Carolina Baraddi , Dr. Viviana Ciambrone , Prof. Mario Maggi
Published: July 2023

Objectives

To assess the correlations between flaccid penile colour-Doppler ultrasound (fPCDU) parameters, carotid colour-Doppler ultrasound (CCDU) parameters and history of major adverse cardiovascular events (MACE) in men with erectile dysfunction (ED).

Methods

A consecutive series of 269 men (55.3±11.6 years) complaining of ED were evaluated with fPCDU (assessing cavernosal artery peak systolic velocity [CAPSV] and acceleration [CAA]) and CCDU (evaluating intima-media thickness [IMT], the presence of atherosclerotic plaques [AP] at the origin of the internal carotid artery [ICA], and the ratio between ICA-PSV and common carotid artery-PSV [ICA/CCA-PSV ratio], the latter widely used to extimate hemodynamic abnormalities at the ICA level). In addition, the history of MACE of the patients was collected.

Results

At fPCDU, the mean CAPSV was 13.9±4.2 cm/s, the mean CAA was 1.92±0.66 m/s2. At CCDU, the mean IMT was 0.8±0.3 cm/s, 37% of men had ICA-AP and the median ICA/CCA-PSV ratio was 0.7 [0.6-0.8]. Overall, 7.9% of men had a history of MACE (3.7% acute myocardial infarction, 2.4% NSTEMI, 0.7% stroke, 1.1% peripheral arterial thrombosis). At fPCDU, mean CAPSV was positively associated with mean CAA (p<0.0001). At CCDU, mean IMT was positively associated with mean ICA/CCA-PSV ratio (p<0.001). Both mean CAPSV and CAA were negatively associated with mean IMT and ICA/CCA-PSV ratio, respectively (all p < 0.001). Men with ICA-AP showed lower CAPSV and CAA than those without (both p <0.001). Men with a history of MACE showed lower penile CAPSV and CAA (both p<0.05), but no difference in IMT, ICA-AP or ICA/CCA-PSV ratio compared to those without. Al data were confirmed after adjusting for age.

Conclusions

Although fPCDU and CCDU blood flow parameters are associated, subjects with MACE history show worse fPCDU, but not CCDU, blood flow parameters, than those without MACE history. Hence, fPCDU represent a better marker of a worse systemic cardiovascular status respect to CCDU.

Conflicts of Interest

No
(79) SURGICAL AND FUNCTIONAL OUTCOMES OF PENILE PROSTHESIS IMPLANTATION IN MEN WITH NEUROLOGICAL CONDITIONS
Authors: Marco Falcone , Paolo Capogrosso , Lorenzo Cirigliano , Mirko Preto , Massimiliano Timpano , Federica Peretti , Ilaria Ferro , Nicolò Schifano , Carlo Ceruti , Edoardo Pescatori , Enrico Caraceni , Fulvio Colombo , Maurizio Carrino , Carlo Bettocchi , Antonio Vitarelli , Matteo Paradiso , Federico Dehò , Tommaso Cai , Franco Giorgio , Alessandro Palmieri , Prof. Paolo Gontero
Published: July 2023

Objectives

Pharmacological treatment for erectile dysfunction (ED) is not always effective in patients with neurological disease; as such, penile prosthesis implant (PPI) can be a valid treatment option. We investigated outcomes of PPI in neurological patients using the Italian multi-institutional national registry of penile prostheses (INSIST-ED)

Methods

INSIST-ED registry, relating to patients implanted from 2014 to 2021, was analyzed. All data were prospectively recorded by 45 surgeons on a dedicated website (www.registro.andrologiaitaliana.it) and revised by a data-manager. We selected patients with neurological disease undergoing PPI for ED. Follow-up was scheduled at 3,6 and 12 months, then annually. We evaluated postoperative complications and functional outcomes through validated questionnaires (IIEF and EDITS). A non-validated questionnaire was administered to evaluate patient’satisfaction.

Results

33 patients were included with a median age of 52 (IQR: 44.75-55.25). Peno-scrotal approach for PPI was performed in most cases (90.9%) while infra-pubic was used in 3 cases (9.1%). An inflatable and a malleabel device was implanted in 30 (90.9 %) and 3 cases (9.1 %), respectively. Median follow-up was 83 months (IQR: 67-99.5). Intraoperative complications occurred in one case (3%). Early postoperative complications (<90 days) were observed in 4 cases (12.1%): 3 peno-scrotal hematoma (Clavien-Dindo G1), 1 device infection requiring prosthesis explant (Clavien-Dindo G3a). At the last follow-up, 29 prostheses (87.9%) were still in place and well-functioning. Mechanical failure of inflatable PP occurred in 3 cases (9.1%). Median IIEF before surgery was 32 (IQR 30.25-36). At the last follow-up, IIEF was 61.5 (IQR 60.25-64.25), and median EDITS was 45.5 (IQR 39-46.75). Overall, 29 patients (87.9%) affirmed to be fully satisfied with the PPI.

Conclusions

Although PPI in the neurological population has been historically limited by the evidence of common complications and infections, our study showed that PPI represents a safe and effective procedure to treat ED in neurological patients.

Conflicts of Interest

The Authors have no conflict of interest.
(88) PREVALENCE AND PREDICTING FACTORS FOR COMMONLY NEGLECTED SEXUAL SIDE EFFECTS TO LOW-DOSE RATE BRACHYTHERAPY FOR PROSTATE CANCER
Authors: Dr. Sami Beji , Dr. Alexander B Nolsøe , Dr. Christian F Jensen , Peter B Østergren , Jens Sønksen , Rasmus Bisbjerg , Henrik Jakobsen , Mikkel Fode
Published: July 2023

Objectives

To investigate orgasmic dysfunction, urinary incontinence during sexual activity (UIS), changes in penile morphology, and sensory disturbances in the penis following Low-dose Rate Brachytherapy (LDR-B) for prostate cancer.

Methods

Questionnaire-based study in patients who underwent LDR-B at Herlev Hospital from March 2010 through February 2020. The questionnaire included the International Index of Erectile Function (IIEF) and a series of questions on orgasm, UIS, changes in penile morphology, and sensory disturbances in the penis

Results

Overall, 178 patients responded to the questionnaire. The median age was 70 years (51 to 83 years), and the median time since LDR-B was 93 months (21 to 141 months).

Overall, 142 (80%) were sexually active and 126 (70.8%) had erectile dysfunction (ED). Of the sexually active patients, 8 (5.6%) reported anejaculation and 7 (4.9%) reported anorgasmia. Another 67 (46.9%) had decreased orgasmic intensity, while 69 (49.3%) reported an increased time to orgasm. Twenty-six patients (18.3%) had experienced orgasm associated pain with a median visual analog pain score of 2. Considering overlap 44 patients (31.0%) had an unchanged orgasmic function.

Six (3.3%) patients had experienced UIS at least a few times. Penile length loss was reported by 45 patients (25.2%). Seventeen (9.6%) patients reported an altered curvature of their penis and 9 (5%) reported that they had experience painful erection. Thirty-three patients (18.5%) had experienced decreased sensitivity in the penis.

On multivariate analyses ED was the only independent risk factor for altered perception of orgasm (OR of 6.6, p<0.0001), orgasmic pain (OR 5.5, p=0.008), and penile shortening (OR 4.2, p<0.0056). No independent risk factors were identified for UIS or sensory penile disturbances.

Conclusions

Orgasmic dysfunction, changes in penile morphology, and sensory disturbances in the penis are common side effects of LDR-B for prostate cancer. Meanwhile, UIS is only experienced by a small minority.

Conflicts of Interest

None
(64) INTEGRATION OF THE PSYCHO-SEXOLOGICAL ASPECTS IN THE MEDICAL TREATMENT OF A PERSON WITH SYPHILIS INFECTION
Authors: Dr. Mylene Bolmont , Julie Cloppet Mouchet , Mélanie Michaud , Penelope Bornhauser , Laurence Toutous-Trellu
Published: July 2023

Objectives

Syphilis bears the image of a great sexually transmitted disease. Sexual health of the infected person may be affected. Yet, no study explored this aspect. The aim of the present research is to investigate the sexological impact of syphilis in order to improve our knowledge on this aspect, and consequently, to improve the quality of care for our patients and messages on prevention.

Methods

43 patients, with confirmed syphilis were recruited at the venerology consultation of University Hospital of Geneva. They had to participate in a two-step experiment. First, they had to fill in questionnaires concerning their sexual function. Participants were then invited to discuss with the psycho-sexologist during a semi structured interview, to collect detailed qualitative data on the way they experienced their infection sexologically. The study was accepted in the local ethical committee.

Results

All the patients full filled the questionnaires. 8 women and 35 men, aged from 20 to 57 (39.12±10.56) were included. 13/43 were co-infected with HIV.

The results showed that 25/43 (58.1 %) of the patients reported a negative impact of syphilis on sexual life: blockage feeling for a new sexual intercourse, lack of sexual desire, loss of confidence in the actual and or future sexual partners. We also noted changes in sexual practices for 20/43 (46.51%) of the patients, with safer sex or abstinence, but all these changes only lasted for the duration of the treatment. However, results revealed that only 7/43 of the patients (16.3%) reported a short-term impact of syphilis on mental health, and especially feeling of anxiety, limited to the announcement of the diagnosis.

Conclusions

Because there is a short term impact of syphilis on the sexual sphere of the patients, offering an urgent sexological counselling during the early treatment phase will allow better resilience of people encountering sexual difficulties.

Conflicts of Interest

no conflicts of interest.
(300) SEXUAL FUNCTION IN WOMEN WITH POLYCYSTIC OVARY SYNDROME: AN UPDATED SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Hester Pastoor , Hanneke Bolt , Tania Burgert , Jillian Tay , Aya Mousa , Prof. Helena Teede
Published: July 2023

Objectives

To present a comprehensive review of the evidence on sexual function in women with polycystic ovary syndrome (PCOS) compared to women without PCOS.

Methods

A systematic review and meta-analysis of the published literature was conducted. Eight databases were searched from inception to August 24th 2022. Two authors independently screened articles based on pre-defined inclusion (e.g. adequate PCOS diagnosis) and exclusion criteria (e.g. hyperandrogenism caused by other diseases) and assessed their quality, including grading the certainty of the evidence. Meta-analyses, forest plots and publication bias were assessed using Review Manager.

Results

Twenty-seven articles were included, of which 23 used questionnaires and four used visual analogue scales (VAS). The questionnaires showed worse sexual function across most domains in women with PCOS, including arousal (weighted mean difference [WMD]= -0.36, P= 0.002), lubrication (WMD= -0.47, P <0.001), and orgasm (WMD= -0.35, P <0.001). Total sexual function was also significantly lower in PCOS (WMD= -2.42, P <0.001), as was sexual satisfaction (WMD= -1.48, P <0.001). VAS results were not pooled in meta-analysis due to using the same control group across the included studies. However, effects within individual VAS studies demonstrated the negative impact of excess body hair on sexuality, lower sexual attractiveness and lower sexual satisfaction in women with PCOS compared with controls, with no differences in the perceived importance of a satisfying sex life. Certainty of evidence was graded low for all included studies.

Conclusions

Women with PCOS place similar importance on sexual satisfaction as women without PCOS, yet have lower sexual function and sexual satisfaction across a number of domains. Sexual function assessment, and where relevant, psychosexual counseling should be considered as part of standard care in women with PCOS. Findings from this review have directly informed the international PCOS guideline on sexual function assessment and management.

Conflicts of Interest

HP, JB, TSB, CTT, AM, HJT: no conflicts to report.