bluuebunny/crossref_metadata_2025 huggingface dataset:
Hermeneutika Al-Qur'an dan Debat Tafsir Modern: Implementasinya dengan Masa Kini
Authors: Malik Rif'atul Khoiriah
Published: August 2019

The interpretation of the Quran which triggered controversial themes in the post-modern era is now an interesting study to be studied. As in this paper, hermeneutics as a new method of interpretation that emerged in Ancient Greece has become part of the interpretation method of the Quran. From this arises a debate over the emergence of the hermeneutical method if applied to the Quran. Will the hermeneutic approach be used in the interpretation of the Quran? How does hermeneutics answer the modern interpretation debate? From this question, the author examines some views on hermeneutics among interpreters, accompanied by the interpretation of several controversial themes that occur in modern societies. Through this paper, it will be seen that, the knowledge that continues to be a reformer in the modern era is something that should not be excluded or rejected. With the aim of scientific upgrading, it is increasingly evolving in time and places, but from that it does not forget the old traditions of returning to the Quran and Hadith. Keywords: Hermeneutics, Alquran, Modern Interpretation.
Acceptance and Commitment Therapy Preceded by Attention Bias Modification on Residual Symptoms in Depression: A 12-Month Follow-Up
Authors: Østergaard Tom , Lundgren Tobias , Rosendahl Ingvar , Zettle Robert D. , Jonassen Rune , Harmer Catherine J. , Stiles Tore C. , Landrø Nils Inge , Haaland Vegard Øksendal
Published: August 2019

Depression is a highly recurrent disorder with limited treatment alternatives for reducing risk of subsequent episodes. Acceptance and commitment therapy (ACT) and attention bias modification (ABM) separately have shown some promise in reducing depressive symptoms. This study investigates (a) if group-based ACT had a greater impact in reducing residual symptoms of depression over a 12-month follow-up than a control condition, and (b) if preceding ACT with ABM produced added benefits. This multisite study consisted of two phases. In phase 1, participants with a history of depression, currently in remission (N= 244), were randomized to either receive 14 days of ABM or a control condition. In phase 2, a quasi- experimental design was adopted, and only phase-1 participants from the Sørlandet site (N= 124) next received an 8-week group-based ACT intervention. Self-reported and clinician-rated depression symptoms were assessed at baseline, immediately after phase 1 and at 1, 2, 6, and 12 months after the conclusion of phase 1. At 12-month follow-up, participants who received ACT exhibited fewer self-reported and clinician-rated depressive symptoms. There were no significant differences between ACT groups preceded by ABM or a control condition. There were no significant differences between ACT groups preceded by ABM or a control condition. Group-based ACT successfully decreased residual symptoms in depression over 12 months, suggesting some promise in preventing relapse.
Machine Learning-Based Spectral Library for Crop Classification and Status Monitoring
Authors: Zhang Jingcheng , He Yuhang , Yuan Lin , Liu Peng , Zhou Xianfeng , Huang Yanbo
Published: August 2019

The establishment and application of a spectral library is a critical step in the standardization and automation of remote sensing interpretation and mapping. Currently, most spectral libraries are designed to support the classification of land cover types, whereas few are dedicated to agricultural remote sensing monitoring. Here, we gathered spectral observation data on plants in multiple experimental scenarios into a spectral database to investigate methods for crop classification (16 crop species) and status monitoring (tea plant and rice growth). We proposed a set of screening methods for spectral features related to plant classification and status monitoring (band reflectance, vegetation index, spectral differentiation, spectral continuum characteristics) that are based on ISODATA and JM distance. Next, we investigated the performance of different machine learning classifiers in the spectral library application, including K-nearest neighbor (KNN), Random Forest (RF), and a genetic algorithm coupled with a support vector machine (GA-SVM). The optimal combination of spectral features and the classifier with the highest classification accuracy were selected for crop classification and status monitoring scenarios. The GA-SVM classifier performed the best, which produced an accuracy of OAA = 0.94, Kappa = 0.93 for crop classification in a complex scenario (crops mixed with 71 non-crop plant species), and promising accuracies for tea plant growth monitoring (OAA = 0.98, Kappa = 0.97) and rice growth stage monitoring (OAA = 0.92, Kappa = 0.90). Therefore, the establishment of a plant spectral library combined with relevant feature extraction and a classification algorithm effectively supports agricultural monitoring by remote sensing.
Effects of Dietary Aged Maize with Oxidized Fish Oil on Growth Performance, Antioxidant Capacity and Intestinal Health in Weaned Piglets
Authors: Luo Bin , Chen Daiwen , Tian Gang , Zheng Ping , Yu Jie , He Jun , Mao Xiangbin , Luo Yuheng , Luo Junqiu , Huang Zhiqing , Yu Bing
Published: August 2019

This study aimed to determine the effects of dietary aged maize with supplementation of different levels of oxidized fish oil on growth performance, nutrient digestibility, serum antioxidant activity and gut health in piglets. Forty-two piglets were arranged in 2 × 3 factorial treatments in a complete randomized block design with seven replicates per treatment and one pig per replicate for 28 d. Diets included twp types of maize (normal maize or aged maize) and three levels of oxidized fish oil (OFO) (3% non-oxidized fish oil (0% OFO), 1.5% OFO and 1.5% non-oxidized fish oil (1.5% OFO), and 3% OFO (3% OFO). Results showed that dietary aged maize did not affect growth performance, diarrhea, and the apparent total tract digestibility (ATTD) of nutrients in piglets (p > 0.05). However, aged maize increased malonaldehyde (MDA) content and decreased total antioxidant capacity (T-AOC) in serum on both 14th and 28th days (p < 0.05) compared to the normal maize groups. Meanwhile, compared with normal maize, dietary aged maize showed a slight, but not significant (p > 0.10) decrease in total superoxide dismutase (T-SOD) activity and VE content in serum on the 14th day. In addition, aged maize significantly decreased GLUT2 mRNA expression (p < 0.05) and tended to increase (p < 0.10) TNF-α and IL-6 mRNA expression in jejunal mucosa. Compared with non-oxidized fish oil, oxidized fish oil resulted in the decrease of the 14–28 d and 0–28 d ADG, as well as the ATTD of dry matter (DM), ether extract (EE), organic matter (OM) (p < 0.05), whereas the increase in diarrhea index (p < 0.05) and F/G of the whole period (p < 0.05). Oxidized fish oil decreased serum T-AOC on both the 14th and the 28th days (p < 0.05), and decreased serum T-SOD activity and VE content on the 28th day (p < 0.05), whereas increased serum MDA content on the 28th day (p < 0.05) and 14th day (p < 0.10) compared with fresh fish oil. Meanwhile, MUC2 (p < 0.05) and SGLT1 (p < 0.10) mRNA expression in jejunal mucosa were decreased compared with non-oxidized fish oil. In addition, dietary oxidized fish oil tended to decrease 14–28 d ADFI and the ATTD of CP (p < 0.10), and piglets fed oxidized fish oil significantly decreased 14–28 d ADFI, the ATTD of CP, GLUT2 and SGLT1 mRNA expressions in jejunal mucosa when piglet also fed with aged maize (p < 0.05). Collectively, these results indicated that dietary oxidized fish oil decreased growth performance and nutrients digestibility of piglets fed with aged maize. This nutrient interaction may be mediated by inhibiting intestinal nutrient transporter, inducing intestinal inflammation, and reducing antioxidant capacity.
Effects of Supplementation with Royal Poinciana Seed Meal (Delonix regia) on Ruminal Fermentation Pattern, Microbial Protein Synthesis, Blood Metabolites and Mitigation of Methane Emissions in Native Thai Beef Cattle
Authors: Cherdthong Anusorn , Prachumchai Rittikeard , Wanapat Metha , Foiklang Suban , Chanjula Pin
Published: August 2019

The object of this present work was to determine the effects of supplementation with pellets containing royal poinciana seed meal (PEREM) on feed use, ruminal fermentation efficiency, microbial protein synthesis, blood metabolites and mitigation of methane (CH4) emissions in cattle. The animals used in this experiment were four male Thai native beef cattle (Bos indicus) with initial body weights (BWs) of 125 ± 5.0 kg. Each of the animals were randomly assigned to receive PEREM doses at 0, 50, 100 and 150 g/d, respectively, according to a 4 × 4 Latin square design. Concentrates were fed at 0.5% BW daily, and rice straw was fed ad libitum. There were no significant differences (p > 0.05) on intakes of rice straw, concentrate and total diet. The intake of nutrients did not change among the levels of PEREM supplementation (p > 0.05), except for an intake of crude protein, which was linearly enhanced when increasing the dose of PEREM (p < 0.05). The inclusion of different doses of PEREM did not adversely affect the digestibility of dry matter, organic matter, crude protein, neutral detergent fiber and acid detergent fiber (p > 0.05). Adding various doses of PEREM did not alter ruminal pH and ruminal temperature, while concentrations of ammonia-nitrogen were significantly increased with an increased dose of PEREM supplementation (p < 0.01). The increasing doses of PEREM linearly reduced protozoal numbers (p < 0.01), with the lowest concentration when PEREM was added at 150 g. PEREM supplementation did not change (p > 0.05) the concentration of acetic acid or butyric acid or the ratio of acetic acid to propionic acid. Nevertheless, the total volatile fatty acid and propionic acid content were changed among PEREM levels (p < 0.05), which were linearly increased with an increasing dose of PEREM. At 4 h post feeding, the CH4 concentrations in the rumen of the animal were linearly reduced when the dose of pellets was increased (p < 0.01). In addition, the inclusion of PEREM did not adversely affect other blood metabolites, namely total protein, creatinine and albumin (p > 0.05). Furthermore, microbial crude protein and efficiency of microbial N synthesis were linearly enhanced when increasing levels of PEREM were added. The feeding of PEREM at 150 g/d might be an alternative with the potential to improve rumen fermentation efficiency and reduce the environmental effects produced by ruminants.
Proposal of Laser-Induced Ultrasonic Guided Wave for Corrosion Detection of Reinforced Concrete Structures in Fukushima Daiichi Nuclear Power Plant Decommissioning Site
Authors: Furusawa Akinori , Takenaka Yusuke , Nishimura Akihiko
Published: August 2019

Remote-controlled, non-destructive testing is necessary to detect corrosion of the reinforced concrete structures at the Fukushima Daiichi Nuclear Power Plant (NPP) de-commissioning site. This work aims to demonstrate that laser-induced ultrasonic guided wave technology can be applied to achieve this task. Hence, accelerated electrolytic corrosion is performed on a reinforced concrete specimen fabricated by embedding a steel rod into mortar. Waveforms of the laser-induced ultrasonic guided wave on the rod are measured with a previously employed piezoelectric transducer (PZT) probe, for each fixed corrosion time. Based on the results of Fourier and wavelet transforms of the waveforms, issues concerning the detection and extent of rebar corrosion are discussed. It is exhibited that the changes in bonding strength due to corrosion are distinguishable in the frequency domain of the ultrasonic signal.
Application of Non-Thermal Plasma on Biofilm: A Review
Authors: Gupta Tripti Thapa , Ayan Halim
Published: August 2019

The formation of bacterial biofilm on implanted devices or damaged tissues leads to biomaterial-associated infections often resulting in life-threatening diseases and implant failure. It is a challenging process to eradicate biofilms as they are resistant to antimicrobial treatments. Conventional techniques, such as high heat and chemicals exposure, may not be suitable for biofilm removal in nosocomial settings. These techniques create surface degradation on the treated materials and lead to environmental pollution due to the use of toxic chemicals. A novel technique known as non-thermal plasma has a great potential to decontaminate or sterilize those nosocomial biofilms. This article aims to provide readers with an extensive review of non-thermal plasma and biofilms to facilitate further investigations. A brief introduction summarizes the problem caused by biofilms in hospital settings with current techniques used for biofilm inactivation followed by the literature review strategy. The remainder of the review discusses plasma and its generation, the role played by plasma reactive species, various factors affecting the antimicrobial efficacy of non-thermal plasma and summarizes many studies published in the field.
Learning to Build Natural Audio Production Interfaces
Authors: Pardo Bryan , Cartwright Mark , Seetharaman Prem , Kim Bongjun
Published: August 2019

Improving audio production tools provides a great opportunity for meaningful enhancement of creative activities due to the disconnect between existing tools and the conceptual frameworks within which many people work. In our work, we focus on bridging the gap between the intentions of both amateur and professional musicians and the audio manipulation tools available through software. Rather than force nonintuitive interactions, or remove control altogether, we reframe the controls to work within the interaction paradigms identified by research done on how audio engineers and musicians communicate auditory concepts to each other: evaluative feedback, natural language, vocal imitation, and exploration. In this article, we provide an overview of our research on building audio production tools, such as mixers and equalizers, to support these kinds of interactions. We describe the learning algorithms, design approaches, and software that support these interaction paradigms in the context of music and audio production. We also discuss the strengths and weaknesses of the interaction approach we describe in comparison with existing control paradigms.
The Persistence of Primitivism: Equivocation in Ernesto Neto’s A Sacred Place and Critical Practice
Authors: Maroja Camila
Published: August 2019

During the 2017 Venice Biennale, the area dubbed the “Pavilion of the Shamans” opened with A Sacred Place, an immersive environmental work created by the Brazilian artist Ernesto Neto in collaboration with the Huni Kuin, a native people of the Amazon rainforest. Despite the co-authorship of the installation, the artwork was dismissed by art critics as engaging in primitivism and colonialism. Borrowing anthropologist Eduardo Viveiros de Castro’s concept of equivocation, this article examines the incorporation of both indigenous and contemporary art practices in A Sacred Place. The text ultimately argues that a more equivocal, open interpretation of the work could lead to a better understanding of the work and a more self-reflexive global art history that can look at and learn from at its own comparative limitations.
Spatial Distributions and Sources of Inorganic Chlorine in PM2.5 across China in Winter
Authors: Luo Li , Zhang Yong-Yun , Xiao Hua-Yun , Xiao Hong-Wei , Zheng Neng-Jian , Zhang Zhong-Yi , Xie Ya-Jun , Liu Cheng
Published: August 2019

Chlorine is an important atmospheric photochemical oxidant, but few studies have focused on atmospheric chlorine. In this study, PM2.5 samples were collected from urban and rural sites across China in January 2018, and concentrations of Cl− and other water-soluble ions in PM2.5 were analyzed. The size-segregated aerosol Cl− data measured across Chinese cities by other studies were compiled for comparison. The observed data demonstrated that the Cl− concentrations of PM2.5 in northern cities (5.0 ± 3.7 µg/m3) were higher than those in central (1.9 ± 1.2 µg/m3) and southern cities (0.84 ± 0.54 µg/m3), suggesting substantial chlorine emissions in northern cities during winter. The concentrations of Cl− in aerosol were significantly higher in urban regions (0.11–26.7 µg/m3) compared to than in rural regions (0.03–0.61 µg/m3) across China during winter, implying strong anthropogenic chlorine emission in cities. Based on the mole ratios of Cl−/Na+, Cl−/K+ and Cl−/ SO 4 2 − and the PMF model, Cl− in northern and central cities was mainly sourced from the coal combustion and biomass burning, but in southern cities, Cl− in PM2.5 was mainly affected by the equilibrium between gas-phase HCl and particulate Cl−. The size-segregated statistical data demonstrated that particulate Cl− had a bimodal pattern, and more Cl− was distributed in the fine model than that in the coarse mode in winter, with the opposite pattern was observed in summer. This may be attributed to both sources of atmospheric Cl− and Cl− involved in chemical processes. This study reports the concentrations of aerosol Cl− on a national scale, and provides important information for modeling the global atmospheric reactive chlorine distribution and the effects of chlorine on atmospheric photochemistry.
In-house Implementation of Tumor Mutational Burden Testing to Predict Durable Clinical Benefit in Non-small Cell Lung Cancer and Melanoma Patients
Authors: Heeke Simon , Benzaquen Jonathan , Long-Mira Elodie , Audelan Benoit , Lespinet Virginie , Bordone Olivier , Lalvée Salomé , Zahaf Katia , Poudenx Michel , Humbert Olivier , Montaudié Henri , Dugourd Pierre-Michel , Chassang Madleen , Passeron Thierry , Delingette Hervé , Marquette Charles-Hugo , Hofman Véronique , Stenzinger Albrecht , Ilié Marius , Hofman Paul
Published: August 2019

Tumor mutational burden (TMB) has emerged as an important potential biomarker for prediction of response to immune-checkpoint inhibitors (ICIs), notably in non-small cell lung cancer (NSCLC). However, its in-house assessment in routine clinical practice is currently challenging and validation is urgently needed. We have analyzed sixty NSCLC and thirty-six melanoma patients with ICI treatment, using the FoundationOne test (FO) in addition to in-house testing using the Oncomine TML (OTML) panel and evaluated the durable clinical benefit (DCB), defined by >6 months without progressive disease. Comparison of TMB values obtained by both tests demonstrated a high correlation in NSCLC (R2 = 0.73) and melanoma (R2 = 0.94). The association of TMB with DCB was comparable between OTML (area-under the curve (AUC) = 0.67) and FO (AUC = 0.71) in NSCLC. Median TMB was higher in the DCB cohort and progression-free survival (PFS) was prolonged in patients with high TMB (OTML HR = 0.35; FO HR = 0.45). In contrast, we detected no differences in PFS and median TMB in our melanoma cohort. Combining TMB with PD-L1 and CD8-expression by immunohistochemistry improved the predictive value. We conclude that in our cohort both approaches are equally able to assess TMB and to predict DCB in NSCLC.
Characterizing Human Cell Types and Tissue Origin Using the Benford Law
Authors: Morag Sne , Salmon-Divon Mali
Published: August 2019

Processing massive transcriptomic datasets in a meaningful manner requires novel, possibly interdisciplinary, approaches. One principle that can address this challenge is the Benford law (BL), which posits that the occurrence probability of a leading digit in a large numerical dataset decreases as its value increases. Here, we analyzed large single-cell and bulk RNA-seq datasets to test whether cell types and tissue origins can be differentiated based on the adherence of specific genes to the BL. Then, we used the Benford adherence scores of these genes as inputs to machine-learning algorithms and tested their separation accuracy. We found that genes selected based on their first-digit distributions can distinguish between cell types and tissue origins. Moreover, despite the simplicity of this novel feature-selection method, its separation accuracy is higher than that of the mean-expression level approach and is similar to that of the differential expression approach. Thus, the BL can be used to obtain biological insights from massive amounts of numerical genomics data—a capability that could be utilized in various biomedical applications, e.g., to resolve samples of unknown primary origin, identify possible sample contaminations, and provide insights into the molecular basis of cancer subtypes.
Modified Diamide and Phosphine Oxide Extracting Compounds as Membrane Components for Cross-Sensitive Chemical Sensors
Authors: Khaydukova Maria , Militsyn Danila , Karnaukh Mikhail , Grüner Bohumir , Selucký Pavel , Babain Vasily , Wilden Andreas , Kirsanov Dmitry , Legin Andrey
Published: August 2019

This research is devoted to the development and study of novel cross-sensitive sensors based on modified extracting ligands. According to the previous results of liquid extraction studies, the chemical modification of membrane active components would change the analytical characteristics of a sensor comprising them. The sensing elements of the studied sensors consisted of various derivatives of N,N,N′,N′-tetraoctyldiamide of diglycolic acid (TODGA) and di-phenyl-N,N-di-i-sobutylcarbamoylmethylen phoshine oxide (CMPO) used as neutral carriers, CCD (chlorinated cobalt dicarbollide) as a lipophilic additive, different plasticizers, and poly(vinyl chloride) (PVC) as a polymer. TODGA-based sensors demonstrated a stable and reproducible response towards rare earth cations in acidic media (pH = 2). Changing the concentrations and ratio of neutral carriers and the lipophilic additive, it is possible to modify the sensitivity and selectivity of the sensors towards the same target ions. Bonded ligands, such as cobalt dicarbollide covalently attached to TODGA and CMPO, exhibited lower selectivity and sensitivity to rare earth cations. A possibility to vary the cross-sensitivity patterns of the sensors in a wide range might be of great interest for the development of multisensor systems allowing the simultaneous determination of several analytes in multicomponent solutions.
Lossy Mode Resonance-Based Glucose Sensor with High-κ Dielectric Film
Authors: Lin Yu-Cheng , Chen Liang-Yu , Chiu Fu-Chien
Published: August 2019

In the past, high-κ dielectrics gained much attention because of the constant demand for increasingly smaller semiconductors. At the same time, in the field of optical sensing, high-κ dielectrics are key materials. This study presents the experimental investigations on a lossy mode resonance-based optical planar waveguide (LMROPW) sensor coated with a high-κdielectric of an indium tin oxide (ITO) layer. Two types of sensing structures were fabricated by coating (i) only a single-layer ITO (or bared LMROPW) and (ii) an ITO layer with glucose probes onto the optical planar waveguide (or boronic LMROPW) to detect glucose molecules. The sensing characteristics of these two types of sensors toward the surrounding analyte were determined using different concentrations of glucose solutions. It was found that the bared LMROPW sensor is only suitable for a higher concentration of glucose; the boronic LMROPW sensor with glucose probes on ITO could be applied to a lower-concentration solution to monitor glucose adsorption onto the sensing surface. Furthermore, with the advantages of a simple structure, easy alignment, and suitable production, the LMROPW sensor with a high-κ dielectric surface could be applied in clinical testing and diagnostics.
Nuclear Orthologs Derived from Whole Genome Sequencing Indicate Cryptic Diversity in the Bemisia tabaci (Insecta: Aleyrodidae) Complex of Whiteflies
Authors: de Moya Robert S. , Brown Judith K. , Sweet Andrew D. , Walden Kimberly K. O. , Paredes-Montero Jorge R. , Waterhouse Robert M. , Johnson Kevin P.
Published: August 2019

The Bemisia tabaci complex of whiteflies contains globally important pests thought to contain cryptic species corresponding to geographically structured phylogenetic clades. Although mostly morphologically indistinguishable, differences have been shown to exist among populations in behavior, plant virus vector capacity, ability to hybridize, and DNA sequence divergence. These differences allow for certain populations to become invasive and cause great economic damage in a monoculture setting. Although high mitochondrial DNA divergences have been reported between putative conspecifics of the B. tabaci species complex, there is limited data that exists across the whole genome for this group. Using data from 2184 orthologs obtained from whole genome sequencing (Illumina), a phylogenetic analysis using maximum likelihood and coalescent methodologies was completed on ten individuals of the B. tabaci complex. In addition, automatic barcode gap discovery methods were employed, and results suggest the existence of five species. Although the divergences of the mitochondrial cytochrome oxidase I gene are high among members of this complex, nuclear divergences are much lower in comparison. Single-copy orthologs from whole genome sequencing demonstrate divergent population structures among members of the B. tabaci complex and the sequences provide an important resource to aid in future genomic studies of the group.
Autonomy-Supportive and Controlling Teaching in the Classroom: A Video-Based Case Study
Authors: Jiang Jingwen , Vauras Marja , Volet Simone , Salo Anne-Elina , Kajamies Anu
Published: August 2019

This study explored teachers’ autonomy-supportive and controlling behaviors through video-taped observation in the classroom. Four lessons by two teachers from a secondary school in Finland were videotaped and analyzed using a rigorous coding protocol. It was found that teachers employed both autonomy-supportive and controlling teaching during the same lesson, and even combined them in the same instructional sequence. This finding suggests the complexity of the use of autonomy support and control in the classroom, as well as their context-dependent aspects. The novel finding from this study was that teachers showed error tolerance and creativity to support students’ autonomy. Showing error tolerance and teaching creatively have not been investigated from the perspective of autonomy support in previous research. Furthermore, this study suggested that indirect control and its negative effects on students’ learning and well-being should arouse more concern in future research. Implications for teaching practice concerning supporting students’ autonomy have been provided.
Innovative Strategy for Mixer Design Optimization Based on gm/ID Methodology
Authors: Piccinni Giovanni , Talarico Claudio , Avitabile Gianfranco , Coviello Giuseppe
Published: August 2019

This work introduces a process to optimize the design of a down-conversion mixer using an innovative strategy based on the gm/ID methodology. The proposed process relies on a set of technology-oriented lookup tables to optimize the trade-off between gain, power dissipation, noise, and distortion. The design is implemented using a 0.13 μm CMOS technology, and to the best of our knowledge, it possesses the best (post-layout simulation) figure of merit (FOM) among the works presented in literature. The FOM is defined as the product of gain and third-order intercept divided the product between average noise figure and power dissipation. Finally, the core of the mixer takes only 31 µm by 28 µm and it draws a current of 1 mA from the 1.5 V DC supply.
Design, Analysis, and Modeling of an Isolated Constant-Current to Constant-Voltage Converter in Cabled Underwater Information Networks
Authors: Zhang Zheng , Zhou Xuejun , Wang Xichen , Wu Tianshu
Published: August 2019

The underwater electric energy conversion modules are the equipment for distributing electric energy to the electric instruments in the cabled underwater information networks (CUINs), which are also related to the normal and stable operation of the underwater systems, the energy foundation and an important part of the network platform. Based on the classic push-pull high frequency switching circuit of constant voltage conversion, an isolated constant-current to constant-voltage (CC–CV) converter based on pulse width modulation (PWM) feedback was proposed in this paper. Through the theoretical analysis of the working principle of the CC–CV converter and the modeling of the converter circuit based on the state space method, it was verified that the designed converter can achieve the constant current to constant voltage transformation by high-frequency PWM feedback. Based on Saber simulation software, the circuit model of the CC–CV converter was built, and the feasibility of the design was proved by simulation analysis. According to the isolated high-frequency switching CC–CV converter circuit, the physical prototype was made. By adjusting the resistance value of the output load, the constant voltage (CV) characteristic of the output voltage of the converter was analyzed. Furthermore, the results show that the output voltage of the designed CC–CV converter is stable and the ripple of output voltage is less than 5%, when the input current or output load resistance is adjusted under the CC output status, which has the CV output characteristic. The CC–CV converter has good rationality and feasibility, and is suitable for the future development of the constant current remote power supply system of CUINs.
Autophagy Related Gene (ATG3) is a Key Regulator for Cell Growth, Development, and Virulence of Fusarium oxysporum
Authors: Khalid A. Rehman , Lv Xiulan , Naeem Muhammad , Mehmood Khalid , Shaheen Hamayun , Dong Pan , Qiu Dan , Ren Maozhi
Published: August 2019

Fusarium oxysporum is the most important pathogen of potatoes which causes post-harvest destructive losses and deteriorates the market value of potato tubers worldwide. Here, F. oxysporum was used as a host pathogen model system and it was revealed that autophagy plays a vital role as a regulator in the morphology, cellular growth, development, as well as the pathogenicity of F. oxysporum. Previous studies based upon identification of the gene responsible for encoding the autophagy pathway components from F. oxysporum have shown putative orthologs of 16 core autophagy related-ATG genes of yeast in the genome database which were autophagy-related and comprised of ubiquitin-like protein atg3. This study elucidates the molecular mechanism of the autophagy-related gene Foatg3 in F. oxysporum. A deletion (∆) mutants of F. oxysporum (Foatg3∆) was generated to evaluate nuclear dynamics. As compared to wild type and Foatg3 overexpression (OE) strains, Foatg3∆ strains failed to show positive MDC (monodansylcadaverine) staining which revealed that Foatg3 is compulsory for autophagy in F. oxysporum. A significant reduction in conidiation and hyphal growth was shown by the Foatg3∆ strains resulting in loss of virulence on potato tubers. The hyphae of Foatg3∆ mutants contained two or more nuclei within one hyphal compartment while wild type hyphae were composed of uninucleate hyphal compartments. Our findings reveal that the vital significance of Foatg3 as a key target in controlling the dry rot disease in root crops and potato tubers at the postharvest stage has immense potential of disease control and yield enhancement.
Identification of Glyceraldehyde-3-Phosphate Dehydrogenase Gene as an Alternative Safe Harbor Locus in Pig Genome
Authors: Han Xiaosong , Xiong Youcai , Zhao Changzhi , Xie Shengsong , Li Changchun , Li Xinyun , Liu Xiangdong , Li Kui , Zhao Shuhong , Ruan Jinxue
Published: August 2019

The ectopic overexpression of foreign genes in animal genomes is an important strategy for gain-of-function study and establishment of transgenic animal models. Previous studies showed that two loci (Rosa26 and pH11) were identified as safe harbor locus in pig genomes, which means foreign genes can be integrated into this locus for stable expression. Moreover, integration of a transgene may interfere with the endogenous gene expression of the target locus after the foreign fragments are inserted. Here, we provide a new strategy for efficient transgene knock-in in the endogenous GAPDH gene via CRISPR/Cas9 mediated homologous recombination. This strategy has no influence on the expression of the endogenous GAPDH gene. Thus, the GAPDH locus is a new alternative safe harbor locus in the pig genome for foreign gene knock-ins. This strategy is promising for agricultural breeding and biomedical model applications.
Experimental Study on the Influence of Polypropylene Fiber on the Swelling Pressure Expansion Attributes of Silica Fume Stabilized Clayey Soil
Authors: Tiwari Nitin , Satyam Neelima
Published: August 2019

Expansive soil shows dual swell–shrink which is not suitable for construction. Several mitigating techniques exist to counteract the problem promulgated by expansive clayey soils. This paper explored the potential mecho-chemical reinforcement of expansive clayey soil to mitigate the effect of upward swelling pressure and heave. The polypropylene fiber is randomly distributed in the soil for mechanical stabilization, and the industrial residual silica fume is used as a chemical stabilizer. The experimental analysis was made in three phases which involved tests on mechanically-reinforced expansive soil, using randomly distributed polypropylene fibers with different percentages (0.25%, 0.50%, and 1.00%), and which were 12 mm length. The second phase of experiments was carried out on chemical stabilized expansive soil with different percentages (2%, 4%, and 8%) of silica, and the next phase of the experiment focused on the combination of mecho-chemical stabilization of the expansive soil with different combinations of silica (i.e., 2%, 4%, and 8%) and polypropylene fibers (i.e., 0.25%, 0.50%, and 1.00%). Maximum dry density (MDD), optimum moisture content (OMC), liquid limit (LL), plastic limit (PL), plastic index (PI), grain size, and constant volume swelling pressure tests were performed on unreinforced and reinforced expansive soil, to investigate the effects of polypropylene fiber and silica fume on the engineering properties of expansive clayey soil. The experimental results illustrate that the inclusion of polypropylene fiber has a significant effect on the upward swelling pressure and expansion property of expansive soil. The reduction in the upward swelling pressure and expansion is a function of fiber content. These results also indicated that the use of silica fume caused a reduction in upward swelling potential, and its effect was considerably more than the influence of fiber.
Proposal for an Organ-Specific Chronic Inflammation–Remodeling–Carcinoma Sequence
Authors: Okayasu Isao , Ichinoe Masaaki , Yoshida Tsutomu
Published: August 2019

An organ-specific chronic inflammation–remodeling–carcinoma sequence has been proposed, mainly for the alimentary tract. As representative diseases, gastroesophageal reflux disease, chronic gastritis and inflammatory bowel disease (ulcerative colitis and Crohn’s disease of the colitis type) were adopted for this discussion. Tissue remodeling is such an important part of tumorigenesis in this sequence that an organ-specific chronic inflammation–remodeling–carcinoma sequence has been proposed in detail. Chronic inflammation accelerates the cycle of tissue injury and regeneration; in other words, cell necrosis (or apoptosis) and proliferation result in tissue remodeling in long-standing cases of inflammation. Remodeling encompasses epithelial cell metaplasia and stromal fibrosis, and modifies epithelial–stromal cell interactions. Further, the accumulation of genetic, epigenetic and molecular changes—as well as morphologic disorganization—also occurs during tissue remodeling. The expression of mucosal tissue adapted to chronic inflammatory injury is thought to occur at an early stage. Subsequently, dysplasia and carcinoma develop on a background of remodeling due to continuous, active inflammation. Accordingly, organ-specific chronic inflammation should be ameliorated or well controlled with appropriate monitoring if complete healing is unachievable.
Loss of Glycine N-Methyltransferase Associates with Angiopoietin-Like Protein 8 Expression in High Fat-Diet-Fed Mice
Authors: Huang Jian-Wei , Chen Chao-Ju , Yen Chia-Hung , Chen Yi-Ming Arthur , Liu Yu-Peng
Published: August 2019

Imbalance of lipid metabolism is a main cause of metabolic syndrome leading to life-threatening metabolic diseases. Angiopoietin-like protein 8 (Angptl8) was recently identified as a liver and adipose tissue-released hormone that is one of the molecules involved in triglyceride metabolism. However, the regulatory mechanism of Angptl8 is largely unknown. A high fat diet (HFD)-fed mouse model, which showed high cholesterol, high triglyceride, and high insulin in the blood, revealed the upregulation of hepatic and plasma Angptl8 and the downregulation of hepatic glycine N-methyltransferase (GNMT). The inverse correlation of hepatic Angptl8 and GNMT expression in the livers of HFD-fed mice was also confirmed in a publicly available microarray dataset. The mechanistic study using primary hepatocytes showed that the Angptl8 expression could be induced by insulin treatment in a dose- and time-dependent manner. Inhibition of PI3K/Akt pathway by the specific inhibitors or the dominant-negative Akt blocked the insulin-induced Angptl8 expression. Moreover, knockout of GNMT promoted the Akt activation as well as the Angptl8 expression. These results suggested that GNMT might be involved in insulin-induced Angptl8 expression in HFD-mediated metabolic syndrome.
The Reason for Growth Inhibition of Ulmus pumila ‘Jinye’: Lower Resistance and Abnormal Development of Chloroplasts Slow Down the Accumulation of Energy
Authors: Zuo Lihui , Zhang Shuang , Liu Yichao , Huang Yinran , Yang Minsheng , Wang Jinmao
Published: August 2019

Ulmus pumila ‘Jinye’, the colorful leaf mutant of Ulmus pumila L., is widely used in landscaping. In common with most leaf color mutants, U. pumila ‘Jinye’ exhibits growth inhibition. In this study, U. pumila L. and U. pumila ‘Jinye’ were used to elucidate the reasons for growth inhibition at the physiological, cellular microstructural, and transcriptional levels. The results showed that the pigment (chlorophyll a, chlorophyll b, and carotenoids) content of U. pumila L. was higher than that of U. pumila ‘Jinye’, whereas U. pumila ‘Jinye’ had a higher proportion of carotenoids, which may be the cause of the yellow leaves. Examination of the cell microstructure and RNA sequencing analysis showed that the leaf color and growth inhibition were mainly due to the following reasons: first, there were differences in the structure of the thylakoid grana layer. U. pumila L. has a normal chloroplast structure and clear thylakoid grana slice layer structure, with ordered and compact thylakoids. However, U. pumila ‘Jinye’ exhibited the grana lamella stacking failures and fewer thylakoid grana slice layers. As the pigment carrier and the key location for photosynthesis, the close stacking of thylakoid grana could combine more chlorophyll and promote efficient electron transfer promoting the photosynthesis reaction. In addition, U. pumila ‘Jinye’ had a lower capacity for light energy absorption, transformation, and transportation, carbon dioxide (CO2) fixation, lipopolysaccharide biosynthesis, auxin synthesis, and protein transport. The genes related to respiration and starch consumption were higher than those of U. pumila L., which indicated less energy accumulation caused the growth inhibition of U. pumila ‘Jinye’. Finally, compared with U. pumila ‘Jinye’, the transcription of genes related to stress resistance all showed an upward trend in U. pumila L. That is to say, U. pumila L. had a greater ability to resist adversity, which could maintain the stability of the intracellular environment and maintain normal progress of physiological metabolism. However, U. pumila ‘Jinye’ was more susceptible to changes in the external environment, which affected normal physiological metabolism. This study provides evidence for the main cause of growth inhibition in U. pumila ‘Jinye’, information for future cultivation, and information on the mutation mechanism for the breeding of colored leaf trees.
New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation
Authors: Ohyama Takashi
Published: August 2019

Metal cations are associated with many biological processes. The effects of these cations on nucleic acids and chromatin were extensively studied in the early stages of nucleic acid and chromatin research. The results revealed that some monovalent and divalent metal cations, including Mg2+, profoundly affect the conformations and stabilities of nucleic acids, the folding of chromatin fibers, and the extent of chromosome condensation. Apart from these effects, there have only been a few reports on the functions of these cations. In 2007 and 2013, however, Mg2+-implicated novel phenomena were found: Mg2+ facilitates or enables both self-assembly of identical double-stranded (ds) DNA molecules and self-assembly of identical nucleosomes in vitro. These phenomena may be deeply implicated in the heterochromatin domain formation and chromatin-based phase separation. Furthermore, a recent study showed that elevation of the intranuclear Mg2+ concentration causes unusual differentiation of mouse ES (embryonic stem) cells. All of these phenomena seem to be closely related to one another. Mg2+ seems to be a key regulator of chromatin dynamics and chromatin-based biological processes.
Xenobiotic Receptors and Their Mates in Atopic Dermatitis
Authors: Minzaghi Deborah , Pavel Petra , Dubrac Sandrine
Published: August 2019

Atopic dermatitis (AD) is the most common inflammatory skin disease worldwide. It is a chronic, relapsing and pruritic skin disorder which results from epidermal barrier abnormalities and immune dysregulation, both modulated by environmental factors. AD is strongly associated with asthma and allergic rhinitis in the so-called ‘atopic march’. Xenobiotic receptors and their mates are ligand-activated transcription factors expressed in the skin where they control cellular detoxification pathways. Moreover, they regulate the expression of genes in pathways involved in AD in epithelial cells and immune cells. Activation or overexpression of xenobiotic receptors in the skin can be deleterious or beneficial, depending on context, ligand and activation duration. Moreover, their impact on skin might be amplified by crosstalk among xenobiotic receptors and their mates. Because they are activated by a broad range of endogenous molecules, drugs and pollutants owing to their promiscuous ligand affinity, they have recently crystalized the attention of researchers, including in dermatology and especially in the AD field. This review examines the putative roles of these receptors in AD by critically evaluating the conditions under which the proteins and their ligands have been studied. This information should provide new insights into AD pathogenesis and ways to develop new therapeutic interventions.
Effective Treatment of Metastatic Melanoma by Combining MAPK and PI3K Signaling Pathway Inhibitors
Authors: Nymark Aasen Synnøve , Parajuli Himalaya , Hoang Tuyen , Feng Zichao , Stokke Krister , Wang Jiwei , Roy Kislay , Bjerkvig Rolf , Knappskog Stian , Thorsen Frits
Published: August 2019

Malignant melanoma is the most aggressive type of skin cancer and is closely associated with the development of brain metastases. Despite aggressive treatment, the prognosis has traditionally been poor, necessitating improved therapies. In melanoma, the mitogen activated protein kinase and the phosphoinositide 3-kinase signaling pathways are commonly altered, and therapeutically inhibiting one of the pathways often upregulates the other, leading to resistance. Thus, combined treatment targeting both pathways is a promising strategy to overcome this. Here, we studied the in vitro and in vivo effects of the PI3K inhibitor buparlisib and the MEK1/2 inhibitor trametinib, used either as targeted monotherapies or in combination, on patient-derived melanoma brain metastasis cell lines. Scratch wound and trans-well assays were carried out to assess the migratory capacity of the cells upon drug treatment, whereas flow cytometry, apoptosis array and Western blots were used to study apoptosis. Finally, an in vivo treatment experiment was carried out on NOD/SCID mice. We show that combined therapy was more effective than monotherapy. Combined treatment also more effectively increased apoptosis, and inhibited tumor growth in vivo. This suggests a clinical potential of combined treatment to overcome ceased treatment activity which is often seen after monotherapies, and strongly encourages the evaluation of the treatment strategy on melanoma patients with brain metastases.
Cyclin–CDK Complexes are Key Controllers of Capacitation-Dependent Actin Dynamics in Mammalian Spermatozoa
Authors: Bernabò Nicola , Ramal-Sanchez Marina , Valbonetti Luca , Machado-Simoes Juliana , Ordinelli Alessandra , Capacchietti Giulia , Taraschi Angela , Barboni Barbara
Published: August 2019

Mammalian spermatozoa are infertile immediately after ejaculation and need to undergo a functional maturation process to acquire the competence to fertilize the female egg. During this process, called capacitation, the actin cytoskeleton dramatically changes its organization. First, actin fibers polymerize, forming a network over the anterior part of the sperm cells head, and then it rapidly depolymerizes and disappears during the exocytosis of the acrosome content (the acrosome reaction (AR)). Here, we developed a computational model representing the actin dynamics (AD) process on mature spermatozoa. In particular, we represented all the molecular events known to be involved in AD as a network of nodes linked by edges (the interactions). After the network enrichment, using an online resource (STRING), we carried out the statistical analysis on its topology, identifying the controllers of the system and validating them in an experiment of targeted versus random attack to the network. Interestingly, among them, we found that cyclin-dependent kinase (cyclin–CDK) complexes are acting as stronger controllers. This finding is of great interest since it suggests the key role that cyclin–CDK complexes could play in controlling AD during sperm capacitation, leading us to propose a new and interesting non-genomic role for these molecules.
Correlations and How to Interpret Them
Authors: Atmanspacher Harald , Martin Mike
Published: August 2019

Correlations between observed data are at the heart of all empirical research that strives for establishing lawful regularities. However, there are numerous ways to assess these correlations, and there are numerous ways to make sense of them. This essay presents a bird’s eye perspective on different interpretive schemes to understand correlations. It is designed as a comparative survey of the basic concepts. Many important details to back it up can be found in the relevant technical literature. Correlations can (1) extend over time (diachronic correlations) or they can (2) relate data in an atemporal way (synchronic correlations). Within class (1), the standard interpretive accounts are based on causal models or on predictive models that are not necessarily causal. Examples within class (2) are (mainly unsupervised) data mining approaches, relations between domains (multiscale systems), nonlocal quantum correlations, and eventually correlations between the mental and the physical.