bluuebunny/crossref_metadata_2025 huggingface dataset:
Forensic Post-Mortem Investigation in AAS Abusers: Investigative Diagnostic Protocol. A Systematic Review
Authors: Esposito Massimiliano , Licciardello Gabriele , Privitera Federico , Iannuzzi Salvatore , Liberto Aldo , Sessa Francesco , Salerno Monica
Published: July 2021

Anabolic–androgenic steroids (AASs) are a group of synthetic molecules derived from testosterone and its precursors. AASs are widely used illicitly by adolescents and athletes, especially by bodybuilders; AASs are among the most used drugs for improving physical performance, as well as for aesthetic purposes. The use of AASs by professional and recreational athletes is increasing worldwide. This review focused on deaths related to AAS abuse and to investigation of the autopsy results and histopathological findings using a rigorous methodology protocol covering: a complete autopsy, histological analysis, and a broad toxicological investigation. Moreover, we aimed to define an investigative diagnostic protocol supporting forensic pathologists during the post-mortem investigation of AAS abusers. This review was conducted using PubMed Central and Google Scholar databases to find articles published between 1 January 1968 and 30 June 2021, using the following key terms: “(anabolic-androgenic steroids) AND (autopsy); (anabolic-androgenic steroids) AND (forensic)”. A total of 939 articles were screened and 926 did not meet the inclusion criteria. In conclusion, 14 articles were included in this systematic review, reporting 137 fatal cases of AAS abuse in total. The histopathologic studies showed myocardial damage characterized by myocyte hypertrophy, focal myocyte damage with myofibrillar loss, interstitial fibrosis, mostly subepicardial, and small vessel disease. Indeed, in AAS-related cases, autopsy plays a pivotal role in the study of AAS adverse effects and organ damage related to their use or abuse. This systematic review aimed to define a specific workflow in death cases related to AASs, suggesting important future insights to better clarify sudden deaths related to AASs, such as the use of miRNAs. The forensic community needs a unified approach in cases of suspected death related to the use of AASs. There are several occasions to apply this workflow, for example in cases of death of bodybuilders and of young people who die in gymnasiums or during sports activities.
Walk Your Talk: Real-World Adherence to Guidelines on the Use of MRI in Multiple Sclerosis
Authors: Tortora Mario , Tranfa Mario , D’Elia Anna Chiara , Pontillo Giuseppe , Petracca Maria , Bozzao Alessandro , Caranci Ferdinando , Cervo Amedeo , Cosottini Mirco , Falini Andrea , Longo Marcello , Manara Renzo , Muto Mario , Porcu Michele , Roccatagliata Luca , Todeschini Alessandra , Saba Luca , Brunetti Arturo , Cocozza Sirio , Elefante Andrea
Published: July 2021

(1) Although guidelines about the use of MRI sequences for Multiple Sclerosis (MS) diagnosis and follow-up are available, variability in acquisition protocols is not uncommon in everyday clinical practice. The aim of this study was to evaluate the real-world application of MS imaging guidelines in different settings to clarify the level of adherence to these guidelines. (2) Via an on-line anonymous survey, neuroradiologists (NR) were asked about MRI protocols and parameters routinely acquired when MS patients are evaluated in their center, both at diagnosis and follow-up. Furthermore, data about report content and personal opinions about emerging neuroimaging markers were also retrieved. (3) A total of 46 participants were included, mostly working in a hospital or university hospital (80.4%) and with more than 10 years of experience (47.9%). We found a relatively good adherence to the suggested MRI protocols regarding the use of T2-weighted sequences, although almost 10% of the participants routinely acquired 2D sequences with a slice thickness superior to 3 mm. On the other hand, a wider degree of heterogeneity was found regarding gadolinium administration, almost routinely performed at follow-up examination (87.0% of cases) in contrast with the current guidelines, as well as a low use of a standardized reporting system (17.4% of cases). (4) Although the MS community is getting closer to a standardization of MRI protocols, there is still a relatively wide heterogeneity among NR, with particular reference to contrast administration, which must be overcome to guarantee an adequate quality of patients’ care in MS.
Severe Cardiac and Metabolic Pathology Induced by Steroid Abuse in a Young Individual
Authors: Tirla Adrian , Vesa Cosmin Mihai , Cavalu Simona
Published: July 2021

Androgenic-Anabolic Steroids (AAS) abuse is known to play an important role in causing the systemic inflammatory response and multiple-organ dysfunction in healthy individuals. Although many of the undesirable effects of steroid abuse have been reported, at present, little is known about the effect of anabolic supplements and the correlation between cardiac and metabolic pathology. This paper presents a case of a 25 year old patient with a complex medical history after 6 months of steroid administration. Myocardial infraction, dyslipidemia, obesity, hyperuricemia, secondary diabetes, and chronic renal disease were identified after clinical and para-clinical examinations. The particularities of this case were interpreted in the context of a literature review, highlighting the effect of multi-organ damage as a result of the uncontrolled use of anabolic steroid supplements.
The Comparative Analysis of Phase Shifting Transformers
Authors: Albrechtowicz Paweł , Szczepanik Jerzy
Published: July 2021

Phase shifting transformers (PSTs) are currently widely used in power systems to control power flow. In this manuscript, the results of the asymmetrical PST (APST) with the in-phase PST (called asymmetrical controllable PST-ACPST) were compared, allowing to control both longitudinal and quadrature voltage. The MATLAB simulation model of the ACPST was built to obtain influence of PST in selected models for selected parameters. Then the 30A laboratory PST device was built in the laboratory. The parameters of the MATLAB model were then adjusted to parameters of the real life PST model. This allowed verifying the results of the real life and computer simulations. Based on the ACPST simulation model, the APST model was built; for the given work conditions, the influence of both PSTs were compared. APST construction always resulted in higher output voltage than the input one. ACPST achieved the same power transfer for the lower output PST voltage, which is its main advantage. This dependency is a result of the greater ACPST angle compared to the classical APST. The ACPST also allowed adjusting longitudinal and quadrature voltages; therefore, this PST type can be installed in places where high flexibility is required, especially in systems with high renewable energy sources penetration.
A Study on the Corrosion Characteristics of Internal Combustion Engine Materials in Second-Generation Jatropha Curcas Biodiesel
Authors: Shahabuddin M. , Mofijur M. , Shuvho Md. Bengir Ahmed , Chowdhury M. A. K. , Kalam M. A. , Masjuki H. H. , Chowdhury M. A.
Published: July 2021

The corrosiveness of biodiesel affects the fuel processing infrastructure and different parts of an internal combustion (IC) engine. The present study investigates the corrosion behaviour of automotive materials such as stainless steel, aluminium, cast iron, and copper in 20% (B20) and 30% (B30) by volume second-generation Jatropha biodiesel using an immersion test. The results were compared with petro-diesel (B0). Various fuel properties such as the viscosity, density, water content, total acid number (TAN), and oxidation stability were investigated after the immersion test using ASTM D341, ASTM D975, ASTM D445, and ASTM D6751 standards. The morphology of the corroded materials was investigated using optical microscopy and scanning electron microscopy SEM), whereas the elemental analysis was carried out using energy-dispersive X-ray spectroscopy (EDS). The highest corrosion using biodiesel was detected in copper, while the lowest was detected in stainless steel. Using B20, the rate of corrosion in copper and stainless steel was 17% and 14% higher than when using diesel, which further increased to 206% and 86% using B30. After the immersion test, the viscosity, water content, and TAN of biodiesel were increased markedly compared to petro-diesel.
Measurement Tests and FEM Calculations of DC Excited Flux Switching Motor Prototype
Authors: Drabek Tomasz , Kara Dawid , Kołacz Tomasz , Lerch Tomasz , Skwarczyński Jerzy
Published: July 2021

The paper presents the results of laboratory tests and FEM 2D calculations of the DCEFSM (Direct Current Excited Flux Switching Machine) electric motor prototype, made based on the stator ferromagnetic sheets of the induction motor. Static measurements of torques, currents, power, efficiency, electromotive forces, and voltages of the motor under various operating conditions were performed, as well as the recordings of its currents and back-EMFs waveforms. FEM calculations of measured values were also performed. The obtained results allow us to conclude that the motor has the operating properties of an under-excited synchronous cylindrical motor and can be vector controlled like the PMSM motor. The results of measurements and calculations indicate the need to redesign the magnetic circuit of the stator.
Trade, Climate and Energy: A New Study on Climate Action through Free Trade Agreements
Authors: Dent Christopher M.
Published: July 2021

Efforts to tackle climate change are taking place on multiple fronts. This includes trade, an increasingly important defining feature of the global economy. In recent years, free trade agreements (FTAs) have become the primary mechanism of trade policy and diplomacy. This study examines the development of climate action measures in FTAs and discusses what difference they can make to tackling climate change. Its primary source research is based on an in-depth examination of FTAs in force up to 2020. This paper is structured around a number of research questions forming around three main inter-related areas of enquiry. Firstly, to what extent are these provisions in FTAs essentially derivative of energy’s connections with climate change, and thus part of a wider trade–climate–energy nexus? Secondly, what kinds of climate action are FTAs specifically promoting, and how effective a potential positive impact may we expect these to have? Thirdly, are certain climate action norms being promoted by trade partners in FTAs and if so, then who are the norm leaders, what is motivating them, and to what extent are they extending their influence over other trade partners? In addressing these questions, this study offers new insights and analysis regarding a potentially important emerging trend in the trade–climate–energy nexus. Its international political economy approach and latest empirical research also provide a further distinctive contribution to knowledge in this inter-disciplinary area, developing new comprehensions of the relationship between trade, climate action and energy.
Positioning Bio-Based Energy Systems in a Hypercomplex Decision Space—A Case Study
Authors: Oláh Judit , Popp József , Duleba Szabolcs , Kiss Anna , Lakner Zoltán
Published: July 2021

The optimization of the energy portfolio for a small, open, landlocked economy with rather limited fossil resources is a complex task because it must find a long-range, sustainable balance between the various goals of society under the constant pressure of different interest groups. The opinions of independent, informed experts could be an essential input in the decision-making process. The goal of this research was to determine the relative importance of the values and goals potentially accompanying projects, based on the utilization of bioenergy. The current research is based on a wide-ranging survey of 65 non-partisan experts, applying the Pareto analytic hierarchy process to ensure the unbiased prioritization of project segments. The results of the survey put a spotlight on the importance of the economic role of bioenergy projects. Contrary to previous expectations and considerations, the social functions of these projects have hitherto been given relatively little importance. The results highlight the importance of bioenergy in increasing the income-generating capacity of agricultural producers by optimal utilization of natural resources for agricultural production. This can be achieved without considerable deterioration of the natural environment. Modern agricultural production is characterized by high levels of mechanization and automatization. Under these conditions, the social role of bioenergy projects (job creation) is rather limited.
A Novel Method for Structural Lightweight Design with Topology Optimization
Authors: Xue Hongjun , Yu Haiyang , Zhang Xiaoyan , Quan Qi
Published: July 2021

Topological optimization is an innovative method to realize the lightweight design. This paper proposes a hybrid topology optimization method that combines the SIMP (solid isotropic material with penalization) method and genetic algorithm (GA), called the SIMP-GA method. In the method, SIMP is used to update the chromosomes, which can accelerate convergence. The filtering scheme in the SIMP method can filter unconnected elements to ensure the connectivity of the structure. We studied the influence of varying the filtering radius on the optimized structure. Simultaneously, in the SIMP-GA method, each element is regarded as a gene, which controls the population number to a certain extent, reduces the amount of calculation, and improves the calculation efficiency. The calculation of some typical examples proves that the SIMP-GA method can obtain a better solution than the gradient-based method. Compared with the conventional genetic algorithm and GA-BESO (Bi-directional Evolutionary Structural Optimization) method, the calculation efficiency of the proposed method is higher and similar results are obtained. The innovative topology optimization method could be an effective way for structural lightweight design.
Experimental Studies of the Effect of Design and Technological Solutions on the Intensification of an Underground Coal Gasification Process
Authors: Bazaluk Oleg , Lozynskyi Vasyl , Falshtynskyi Volodymyr , Saik Pavlo , Dychkovskyi Roman , Cabana Edgar
Published: July 2021

This paper represents the results of experimental studies of physical modeling of the underground coal gasification process in terms of implementation of design and technological solutions aimed at intensification of a gasification process of thin coal seams. A series of experimental studies were performed in terms of a stand unit with the provided criteria of similarity to field conditions as well as kinetics of thermochemical processes occurring within a gas generator. Hard coal (high volatile bituminous coal) was selected as the raw material to be gasified, as that coal grade prevails in Ukrainian energy balance since it is represented by rather great reserves. Five blow types were tested during the research (air, air–steam, oxygen–steam, oxygen–enriched, and carbon dioxide and oxygen). As a result, the effect of tightness of a gas generator on the quantitative and qualitative parameters of coal gasification while varying the blow by reagents and changing the pressure in a reaction channel has been identified. Special attention was paid to the design solutions involving blow supply immediately into the combustion face of a gas generator. The experimental results demonstrate maximum efficiency of the applied gas generator design involving flexible pipelines and activator in the reaction channel and a blow direction onto the reaction channel face combined with blow stream reversing which will make it possible to improve caloricity of the generator gas up to 18% (i.e., from 8.4 to 12.8 MJ/m3 depending upon a blow type). Consideration of the obtained results of physical modelling can be used with sufficient accuracy to establish modern enterprises based on the underground coal seam gasification; this will help develop more efficiently the substandard coal reserves to generate heat energy as well as power-producing and chemical raw material. The research conclusions can provide technical reference for developing a new generation of underground coal gasification technology.
A Family of Transformerless Quadratic Boost High Gain DC-DC Converters
Authors: Zaid Mohammad , Lin Chang-Hua , Khan Shahrukh , Ahmad Javed , Tariq Mohd , Mahmood Arshad , Sarwar Adil , Alamri Basem , Alahmadi Ahmad
Published: July 2021

This paper presents three new and improved non-isolated topologies of quadratic boost converters (QBC). Reduced voltage stress across switching devices and high voltage gain with single switch operation are the main advantages of the proposed topologies. These topologies utilize voltage multiplier cells (VMC) made of switched capacitors and switched inductors to increase the converter’s voltage gain. The analysis in continuous conduction mode is discussed in detail. The proposed converter’s voltage gain is higher than the conventional quadratic boost converter, and other recently introduced boost converters. The proposed topologies utilize only a single switch and have continuous input current and low voltage stress across switch, capacitors, and diodes, which leads to the selection of low voltage rating components. The converter’s non-ideal voltage gain is also determined by considering the parasitic capacitance and ON state resistances of switch and diodes. The efficiency analysis incorporating switching and conduction losses of the switching and passive elements is done using PLECS software (Plexim, Zurich, Switzerland). The hardware prototype of the proposed converters is developed and tested for verification.
Comparative TCO Analysis of Battery Electric and Hydrogen Fuel Cell Buses for Public Transport System in Small to Midsize Cities
Authors: Kim Hanhee , Hartmann Niklas , Zeller Maxime , Luise Renato , Soylu Tamer
Published: July 2021

This paper shows the results of an in-depth techno-economic analysis of the public transport sector in a small to midsize city and its surrounding area. Public battery-electric and hydrogen fuel cell buses are comparatively evaluated by means of a total cost of ownership (TCO) model building on historical data and a projection of market prices. Additionally, a structural analysis of the public transport system of a specific city is performed, assessing best fitting bus lines for the use of electric or hydrogen busses, which is supported by a brief acceptance evaluation of the local citizens. The TCO results for electric buses show a strong cost decrease until the year 2030, reaching 23.5% lower TCOs compared to the conventional diesel bus. The optimal electric bus charging system will be the opportunity (pantograph) charging infrastructure. However, the opportunity charging method is applicable under the assumption that several buses share the same station and there is a “hotspot” where as many as possible bus lines converge. In the case of electric buses for the year 2020, the parameter which influenced the most on the TCO was the battery cost, opposite to the year 2030 in where the bus body cost and fuel cost parameters are the ones that dominate the TCO, due to the learning rate of the batteries. For H2 buses, finding a hotspot is not crucial because they have a similar range to the diesel ones as well as a similar refueling time. H2 buses until 2030 still have 15.4% higher TCO than the diesel bus system. Considering the benefits of a hypothetical scaling-up effect of hydrogen infrastructures in the region, the hydrogen cost could drop to 5 €/kg. In this case, the overall TCO of the hydrogen solution would drop to a slightly lower TCO than the diesel solution in 2030. Therefore, hydrogen buses can be competitive in small to midsize cities, even with limited routes. For hydrogen buses, the bus body and fuel cost make up a large part of the TCO. Reducing the fuel cost will be an important aspect to reduce the total TCO of the hydrogen bus.
Can Nuclear Batteries Be Economically Competitive in Large Markets?
Authors: Buongiorno Jacopo , Carmichael Ben , Dunkin Bradley , Parsons John , Smit Dirk
Published: July 2021

We introduce the concept of the nuclear battery, a standardized, factory-fabricated, road transportable, plug-and-play micro-reactor. Nuclear batteries have the potential to provide on-demand, carbon-free, economic, resilient, and safe energy for distributed heat and electricity applications in every sector of the economy. The cost targets for nuclear batteries in these markets are 20–50 USD/MWht (6–15 USD/MMBTU) and 70–115 USD/MWhe for heat and electricity, respectively. We present a parametric study of the nuclear battery’s levelized cost of heat and electricity, suggesting that those cost targets are within reach. The cost of heat and electricity from nuclear batteries is expected to depend strongly on core power rating, fuel enrichment, fuel burnup, size of the onsite staff, fabrication costs and financing. Notional examples of cheap and expensive nuclear battery designs are provided.
Heat Transfer with Phase Change in a Multilayer Construction: Simulation versus Experiment
Authors: Kułakowski Tomasz , Krempski-Smejda Michał , Heim Dariusz
Published: July 2021

The latent heat storage in the layer of phase change material (PCM) exposed to dynamic changes in boundary temperature was investigated numerically and experimentally. The original numerical model of heat transfer with phase change using a mushy volume approach was proposed and validated. The main improvement in the proposed model in comparison to others is that the compaction of the mesh and longitude of the time step were chosen after analysis of its impact in the field of error. The model was tested in the case of thin layer structure of the triple glazing window with one cavity filled with phase change material paraffin RT18HC. The experimental validation was carried out in the climatic chamber under dynamic changes in external temperature (from 10 to 50 °C) in a daily cycle. The highest accuracy was obtained for space discretization of the control volume 1 mm thick (12 CV for 12 mm of PCM layer) and 5 min time step. The obtained RMSE values, although they cannot be directly compared because of the very different approaches to the simulations, show that the proposed algorithm is sufficiently accurate for the assessment of energy storage in the PCM window. Both the simulation and experiment proved that, under specific conditions, implementation of the PCM into the structure resulted in delaying the peak for around 4 h.
An Original Control Strategy of Storage Systems for the Frequency Stability of Autonomous Grids with Renewable Power Generation
Authors: Ippolito Mariano G. , Massaro Fabio , Musca Rossano , Zizzo Gaetano
Published: July 2021

This work examines the operation of the autonomous power system of a geographical island assuming the integration of significant generation shares from renewable energy sources and the installation of the required storage systems. The frequency stability of the system is investigated considering different operating conditions, in terms of load demand and renewable power generation. The main focus of the work is an original control strategy specifically designed for power converters interfacing storage units to the grid. The proposed strategy is based on an extended frequency droop control, which selects specific droop settings depending on the operating mode—charge or discharge—of the storage unit. A simulation model of the whole electrical system is developed for dynamic analysis. The model also implements the possibility of including specific auxiliary frequency controls for synthetic inertia and primary reserve. The results of the simulation and analysis indicate that the proposed control strategy has a significant positive effect, making the storage units able to provide a fundamental and more effective support to the frequency stability of the system. The application of the proposed control strategy to storage units also reduces the need for a contribution to the frequency control from intermittent and variable sources, making the whole system more robust, stable and reliable.
Bacteria Energy Recovery System Using Natural Soil Bacteria in Microbial Fuel Cells
Authors: Brochu Nathaniel , Belanger-Huot Benjamin , Humeniuk Dmytro , Gong Lingling , Amirdehi Mehran Abbaszadeh , Greener Jesse , Miled Amine
Published: July 2021

This paper describes a two-cycle bacteria energy recovery system (BERS) to power two embedded sensors: an ultra-low portable pH sensor and a sound sensor. The designed unit can handle up to seven microbial fuel cells (MFCs) to charge a super-capacitor. This allows the BERS to provide a constant 0.14 mW without further electrical components for signal conditioning. The two cycles were driven with a 100 kΩ load and a 10 Hz frequency. The BERS is also self-powered with an integrated start-up unit to be self-activated when the MFCs charge the energy-storing unit after three days. The BERS powered pH sensor has an error below 5% at 25 ∘C and is able to work continuously while being activated for 4 h. The performances of the pH and sound sensors were determined based on a compromise between accuracy and power consumption.
Neural Network-Based Control for Hybrid PV and Ternary Pumped-Storage Hydro Plants
Authors: Nag Soumyadeep , Lee Kwang Y.
Published: July 2021

The growth in renewable energy integration over the past few years, primarily fueled by the drop in capital cost, has revealed the requirement for more sustainable methods of integration. This paper presents a collocated hybrid plant consisting of solar photovoltaic (PV) and Ternary pumped-storage hydro (TPSH) and designs controls that integrate the PV plant such that the behavior and the controllability of the hybrid plant are similar to those of a conventional plant within operational constraints. The PV array control and hybrid plant control implement a neural–network-based framework to coordinate the response, de-loading, and curtailment of multiple arrays with the response of the TPSH. With the help of the designed controls, a symbiotic relationship is developed between the two energy resources, where the PV compensates for the TPSH nonlinearities and provides required speed of response, while the TPSH firms the PV system and allows the PV to be integrated using its existing infrastructure. Simulations demonstrate that the designed controls enable the PV system to track references, while the TPSH’s firming and shifting transforms the PV system into a base load plant for most of the day and extends its hours of operation.
Assessment of Energy Transition Policy in Taiwan—A View of Sustainable Development Perspectives
Authors: Wang Chun-Kai , Lee Chien-Ming , Hong Yue-Rong , Cheng Kan
Published: July 2021

Energy transition has become a priority for adaptive policy and measures taken in response to climate change around the world. This is an opportunity and a challenge for the Taiwan government to establish a climate-resilient power generation mixed to ensure electricity security as well as climate change mitigation. This study adopted a sustainable development perspective and applied optimal control theory to establish a cost-effective model to evaluate a long-term (2050), climate-resilient power generation mix for Taiwan. Furthermore, this study applies the STIRPAT approach to predict the demand of electricity by 2050 for the demand side management. The results not only showed the share of various power generation mixed, but also recommended the trajectory of electricity saving by 2050.
Hydraulic Evaluation of the Levee System Evolution on the Kurobe Alluvial Fan in the 18th and 19th Centuries
Authors: Ishikawa Tadaharu , Senoo Hiroshi
Published: July 2021

The development process and flood control effects of the open-levee system, which was constructed from the mid-18th to the mid-19th centuries, on the Kurobe Alluvial Fan—a large alluvial fan located on the Japan Sea Coast of Japan’s main island—was evaluated using numerical flow simulation. The topography for the numerical simulation was determined from an old pictorial map in the 18th century and various maps after the 19th century, and the return period of the flood hydrograph was determined to be 10 years judging from the level of civil engineering of those days. The numerical results suggested the followings: The levees at the first stage were made to block the dominant divergent streams to gather the river flows together efficiently; by the completed open-levee system, excess river flow over the main channel capacity was discharged through upstream levee openings to old stream courses which were used as temporary floodways, and after the flood peak, a part of the flooded water returned to the main channel through the downstream levee openings. It is considered that the ideas of civil engineers of those days to control the floods exceeding river channel capacity, embodied in their levee arrangement, will give us hints on how to control the extraordinary floods that we should face in the near future when the scale of storms will increase due to the global climate change.
Dynamic Top Height Growth Models for Eight Native Tree Species in a Cool-Temperate Region in Northeast China
Authors: Hipler Sandra-Maria , Spiecker Heinrich , Wu Shuirong
Published: July 2021

In this study, we developed dynamic top height growth models for the eight important Chinese tree species Larix gmelinii var. principis-rupprechtii, Pinus tabuliformis Carr., Pinus sylvestris var. mongolica Litv., Picea asperata Mast., Quercus mongolica Fisch. ex Ledeb, Betula platyphylla Suk., Betula dahurica Pall. and Populus davidiana Dode based on age-height relationships. For this purpose, commonly growth data from long-term observations of permanent experimental plots are used, which ideally cover all development stages from stand establishment to final harvest. As such data were not available in the research area of Hebei Province in Northeast China, we used stem analysis data as well as tree height and annual shoot length measurements. The dataset consisted of 72 stands, 233 dominant trees and 10,195 observations of stem discs and annual shoot length measurements. Five dynamic base-age invariant top height growth models were derived from four base models with the generalized algebraic difference approach and fitted to our age-height data using nested regression techniques. According to biological plausibility and model accuracy the Chapman–Richards model showed the best performance for Picea asperata. This selected model accounted for 99% of the total variance in age-height relationship with average absolute bias of 0.2322 m, root mean square error of 0.3337 m and Radj2 of 0.9979, respectively. The distribution of the residuals was scattered around 0 and without visible trends, indicating that the fitness of the models was good. All developed models are able to generate top height growth curves representing the analyzed height growth data and can be utilized for predicting height growth on the base of current height and age of dominant trees. Additionally, they are the base for calculating the development of other relevant stand attributes such as basal area and volume growth. The determination of potential site productivity by the use of top height growth curves is a practical and convenient method for a simplified presentation of complex growth processes in stands and helps to create growth models, which facilitate implementing sustainable forest management practices in Mulan Forest.
Fast and Green Method to Control Frauds of Geographical Origin in Traded Cuttlefish Using a Portable Infrared Reflective Instrument
Authors: Currò Sarah , Balzan Stefania , Serva Lorenzo , Boffo Luciano , Ferlito Jacopo Carlo , Novelli Enrico , Fasolato Luca
Published: July 2021

An appropriate seafood origin identification is essential for labelling regulation but also economic and ecological issues. Near infrared (NIRS) reflectance spectroscopy was employed to assess the origins of cuttlefish caught from five fishing FAO areas (Adriatic Sea, northeastern and eastern central Atlantic Oceans, and eastern Indian and western central Pacific Oceans). A total of 727 cuttlefishes of the family Sepiidae (Sepia officinalis and Sepiella inermis) were collected with a portable spectrophotometer (902–1680 nm) in a wholesale fish plant. NIR spectra were treated with standard normal variate, detrending, smoothing, and second derivative before performing chemometric approaches. The random forest feature selection procedure was executed to select the most significative wavelengths. The geographical origin classification models were constructed on the most informative bands, applying support vector machine (SVM) and K nearest neighbors algorithms (KNN). The SVM showed the best performance of geographical classification through the hold-out validation according to the overall accuracy (0.92), balanced accuracy (from 0.83 to 1.00), sensitivity (from 0.67 to 1.00), and specificity (from 0.88 to 1.00). Thus, being one of the first studies on cuttlefish traceability using NIRS, the results suggest that this represents a rapid, green, and non-destructive method to support on-site, practical inspection to authenticate geographical origin and to contrast fraudulent activities of cuttlefish mislabeled as local.
Heat and Mass Transfer Modeling to Predict Temperature Distribution during Potato Frying after Pre-Treatment with Pulsed Electric Field
Authors: Gholamibozanjani Gohar , Leong Sze Ying , Oey Indrawati , Bremer Phil , Silcock Patrick , Farid Mohammed
Published: July 2021

Based on unsteady state heat conduction, a mathematical model has been developed to describe the simultaneous heat and moisture transfer during potato frying. For the first time, the equation was solved using both enthalpy and Variable Space Network (VSN) methods, based on a moving interface defined by the boiling temperature of water in a potato disc during frying. Two separate regions of the potato disc namely fried (crust) and unfried (core), were considered as heat transfer domains. A variable boiling temperature of the water in potato discs was required as an input parameter for the model as the water is evaporated during frying, resulting in an increase in the soluble solid concentration of the potato sample. Pulsed electric field (PEF) pretreatment prior to frying had no significant effect on the measured moisture content, thermal conductivity or frying time compared to potatoes that did not receive a PEF pretreatment. However, a PEF pretreatment at 1.1 kV/cm and 56 kJ/kg reduced the temperature variation in the experimentally measured potato center by up to 30%. The proposed heat and moisture transfer model based on unsteady state heat conduction successfully predicted the experimental measurements, especially when the equation was solved using the enthalpy method.
Interaction of Bioactive Mono-Terpenes with Egg Yolk on Ice Cream Physicochemical Properties
Authors: Gouda Mostafa , Sheng Long , Aadil Rana Muhammad , Liu Yuanyuan , Ma Meihu , Li Xiaoli , He Yong , Munekata Paulo E. S. , Lorenzo José M.
Published: July 2021

Using natural multi-function phytochemicals could be one of the best solutions for clean-label production. In this study, dairy ice creams were prepared containing 14% egg yolk and 0.1% of thymol (THY), trans-cinnamaldehyde (TC), menthol (MEN), or vanillin (VAN). Then, the physical, chemical, and structural characteristics were evaluated. Magnetic resonance imaging (MRI) analysis (a rapid, chemical-free, and non-invasive tool) was carried out to evaluate the water distribution. A multivariate analysis was conducted among all studied variables. According to the results, the overrun of the MEN ice cream was significantly increased as compared to the control sample. The density was also reduced in the MEN sample. Meanwhile, the spreadability (%) of VAN was significantly increased after 6 min as compared to the control treatment. MRI analysis revealed that water distribution was significantly changed in the THY group. The firmness and viscosity of THY samples were significantly increased (p < 0.05). Multivariate analysis indicated that viscosity index and consistency were the top parameters affected by THY. The authors concluded that THY and VAN are promising stabilizers for ice-cream clean production.
Inside OCD: Perspectives on the Value of Storytelling with Individuals with OCD and Family Members
Authors: Pufahl Jeffrey , Nainaparampil Jaison , Mathews Carol A.
Published: July 2021

The Center for Arts in Medicine at the University of Florida (UF) partnered with the UF Center for OCD, Anxiety, and Related Disorders to develop a storytelling program for individuals with obsessive compulsive disorder (OCD) and their families. Over ten weeks, participants shared stories regarding their experiences with OCD and engaged in theater and storytelling exercises. In collaboration with each other and the facilitators, participants workshopped and transformed their stories into a cohesive theatrical performance. Participants performed in front of a live audience and engaged in a post-show discussion with the audience, which focused on the diagnosis of OCD, stigma regarding the illness, and the benefits of the program. Program members participated in a post-program focus group and completed a qualitative and quantitative online survey. Participants reported improved understanding of their OCD, more acceptance from family and friends, less shame and guilt related to their OCD, and more confidence about sharing their OCD stories. Although the program was not designed to be therapeutic, participants also reported therapeutic value. Preliminary findings of this study suggest storytelling programs can lead to a reduction in both self-stigma and community stigma; improvement of understanding of the lived experience of OCD by families, loved ones, and clinicians; and facilitation of interpersonal connections.
Subjectively Measured Streetscape Perceptions to Inform Urban Design Strategies for Shanghai
Authors: Qiu Waishan , Li Wenjing , Liu Xun , Huang Xiaokai
Published: July 2021

Recently, many new studies applying computer vision (CV) to street view imagery (SVI) datasets to objectively extract the view indices of various streetscape features such as trees to proxy urban scene qualities have emerged. However, human perception (e.g., imageability) have a subtle relationship to visual elements that cannot be fully captured using view indices. Conversely, subjective measures using survey and interview data explain human behaviors more. However, the effectiveness of integrating subjective measures with SVI datasets has been less discussed. To address this, we integrated crowdsourcing, CV, and machine learning (ML) to subjectively measure four important perceptions suggested by classical urban design theory. We first collected ratings from experts on sample SVIs regarding these four qualities, which became the training labels. CV segmentation was applied to SVI samples extracting streetscape view indices as the explanatory variables. We then trained ML models and achieved high accuracy in predicting scores. We found a strong correlation between the predicted complexity score and the density of urban amenities and services points of interest (POI), which validates the effectiveness of subjective measures. In addition, to test the generalizability of the proposed framework as well as to inform urban renewal strategies, we compared the measured qualities in Pudong to other five urban cores that are renowned worldwide. Rather than predicting perceptual scores directly from generic image features using a convolution neural network, our approach follows what urban design theory has suggested and confirmed as various streetscape features affecting multi-dimensional human perceptions. Therefore, the results provide more interpretable and actionable implications for policymakers and city planners.
MYC Ran Up the Clock: The Complex Interplay between MYC and the Molecular Circadian Clock in Cancer
Authors: Burchett Jamison B. , Knudsen-Clark Amelia M. , Altman Brian J.
Published: July 2021

The MYC oncoprotein and its family members N-MYC and L-MYC are known to drive a wide variety of human cancers. Emerging evidence suggests that MYC has a bi-directional relationship with the molecular clock in cancer. The molecular clock is responsible for circadian (~24 h) rhythms in most eukaryotic cells and organisms, as a mechanism to adapt to light/dark cycles. Disruption of human circadian rhythms, such as through shift work, may serve as a risk factor for cancer, but connections with oncogenic drivers such as MYC were previously not well understood. In this review, we examine recent evidence that MYC in cancer cells can disrupt the molecular clock; and conversely, that molecular clock disruption in cancer can deregulate and elevate MYC. Since MYC and the molecular clock control many of the same processes, we then consider competition between MYC and the molecular clock in several select aspects of tumor biology, including chromatin state, global transcriptional profile, metabolic rewiring, and immune infiltrate in the tumor. Finally, we discuss how the molecular clock can be monitored or diagnosed in human tumors, and how MYC inhibition could potentially restore molecular clock function. Further study of the relationship between the molecular clock and MYC in cancer may reveal previously unsuspected vulnerabilities which could lead to new treatment strategies.
Cardiomyocyte Proliferation as a Source of New Myocyte Development in the Adult Heart
Authors: Johnson Jaslyn , Mohsin Sadia , Houser Steven R.
Published: July 2021

Cardiac diseases such as myocardial infarction (MI) can lead to adverse remodeling and impaired contractility of the heart due to widespread cardiomyocyte death in the damaged area. Current therapies focus on improving heart contractility and minimizing fibrosis with modest cardiac regeneration, but MI patients can still progress to heart failure (HF). There is a dire need for clinical therapies that can replace the lost myocardium, specifically by the induction of new myocyte formation from pre-existing cardiomyocytes. Many studies have shown terminally differentiated myocytes can re-enter the cell cycle and divide through manipulations of the cardiomyocyte cell cycle, signaling pathways, endogenous genes, and environmental factors. However, these approaches result in minimal myocyte renewal or cardiomegaly due to hyperactivation of cardiomyocyte proliferation. Finding the optimal treatment that will replenish cardiomyocyte numbers without causing tumorigenesis is a major challenge in the field. Another controversy is the inability to clearly define cardiomyocyte division versus myocyte DNA synthesis due to limited methods. In this review, we discuss several studies that induced cardiomyocyte cell cycle re-entry after cardiac injury, highlight whether cardiomyocytes completed cytokinesis, and address both limitations and methodological advances made to identify new myocyte formation.
Molecular Characterization of Carbonic Anhydrase Genes in Lotus japonicus and Their Potential Roles in Symbiotic Nitrogen Fixation
Authors: Wang Longlong , Liang Jianjun , Zhou Yu , Tian Tao , Zhang Baoli , Duanmu Deqiang
Published: July 2021

Carbonic anhydrase (CA) plays a vital role in photosynthetic tissues of higher plants, whereas its non-photosynthetic role in the symbiotic root nodule was rarely characterized. In this study, 13 CA genes were identified in the model legume Lotus japonicus by comparison with Arabidopsis CA genes. Using qPCR and promoter-reporter fusion methods, three previously identified nodule-enhanced CA genes (LjαCA2, LjαCA6, and LjβCA1) have been further characterized, which exhibit different spatiotemporal expression patterns during nodule development. LjαCA2 was expressed in the central infection zone of the mature nodule, including both infected and uninfected cells. LjαCA6 was restricted to the vascular bundle of the root and nodule. As for LjβCA1, it was expressed in most cell types of nodule primordia but only in peripheral cortical cells and uninfected cells of the mature nodule. Using CRISPR/Cas9 technology, the knockout of LjβCA1 or both LjαCA2 and its homolog, LjαCA1, did not result in abnormal symbiotic phenotype compared with the wild-type plants, suggesting that LjβCA1 or LjαCA1/2 are not essential for the nitrogen fixation under normal symbiotic conditions. Nevertheless, the nodule-enhanced expression patterns and the diverse distributions in different types of cells imply their potential functions during root nodule symbiosis, such as CO2 fixation, N assimilation, and pH regulation, which await further investigations.
Untangling the Cooperative Role of Nuclear Receptors in Cardiovascular Physiology and Disease
Authors: Paredes Ana , Santos-Clemente Rocio , Ricote Mercedes
Published: July 2021

The heart is the first organ to acquire its physiological function during development, enabling it to supply the organism with oxygen and nutrients. Given this early commitment, cardiomyocytes were traditionally considered transcriptionally stable cells fully committed to contractile function. However, growing evidence suggests that the maintenance of cardiac function in health and disease depends on transcriptional and epigenetic regulation. Several studies have revealed that the complex transcriptional alterations underlying cardiovascular disease (CVD) manifestations such as myocardial infarction and hypertrophy is mediated by cardiac retinoid X receptors (RXR) and their partners. RXRs are members of the nuclear receptor (NR) superfamily of ligand-activated transcription factors and drive essential biological processes such as ion handling, mitochondrial biogenesis, and glucose and lipid metabolism. RXRs are thus attractive molecular targets for the development of effective pharmacological strategies for CVD treatment and prevention. In this review, we summarize current knowledge of RXR partnership biology in cardiac homeostasis and disease, providing an up-to-date view of the molecular mechanisms and cellular pathways that sustain cardiomyocyte physiology.
Anandamide Concentration-Dependently Modulates Toll-Like Receptor 3 Agonism or UVB-Induced Inflammatory Response of Human Corneal Epithelial Cells
Authors: Angyal Ágnes , Pénzes Zsófia , Alimohammadi Shahrzad , Horváth Dorottya , Takács Lili , Vereb György , Zsebik Barbara , Bíró Tamás , Tóth Kinga Fanni , Lisztes Erika , Tóth Balázs István , Oláh Attila , Szöllősi Attila Gábor
Published: July 2021

Photodamage-induced and viral keratitis could benefit from treatment with novel nonsteroid anti-inflammatory agents. Therefore, we determined whether human corneal epithelial cells (HCECs) express members of the endocannabinoid system (ECS), and examined how the endocannabinoid anandamide (AEA, N-arachidonoyl ethanolamine) influences the Toll-like receptor 3 (TLR3) agonism- or UVB irradiation-induced inflammatory response of these cells. Other than confirming the presence of cannabinoid receptors, we show that endocannabinoid synthesizing and catabolizing enzymes are also expressed in HCECs in vitro, as well as in the epithelial layer of the human cornea in situ, proving that they are one possible source of endocannabinoids. p(I:C) and UVB irradiation was effective in promoting the transcription and secretion of inflammatory cytokines. Surprisingly, when applied alone in 100 nM and 10 μM, AEA also resulted in increased pro-inflammatory cytokine production. Importantly, AEA further increased levels of these cytokines in the UVB model, whereas its lower concentration partially prevented the transcriptional effect of p(I:C), while not decreasing the p(I:C)-induced cytokine release. HCECs express the enzymatic machinery required to produce endocannabinoids both in vitro and in situ. Moreover, our data show that, despite earlier reports about the anti-inflammatory potential of AEA in murine cornea, its effects on the immune phenotype of human corneal epithelium may be more complex and context dependent.