bluuebunny/crossref_metadata_2025 huggingface dataset:
An integrative approach for exploring the nature of fibroepithelial neoplasms
Authors: Yun Jihui , Heo Woohang , Lee Eun-Shin , Na Deukchae , Kang Wonyoung , Kang Jinjoo , Chae Jeesoo , Lee Dakyung , Lee Woochan , Hwang Jinha , Yoo Tae-Kyung , Hong Bok Sil , Son Hye-Youn , Noh Dong-Young , Lee Charles , Moon Hyeong-Gon , Kim Jong-Il
Published: December 2022

Background

Malignant phyllodes tumour (MPT) is a rare breast malignancy with epithelial and mesenchymal features. Currently, there are no appropriate research models or effective targeted therapeutic approaches for MPT.

Methods

We collected fresh frozen tissues from nine patients with MPT and performed whole-exome and RNA sequencing. Additionally, we established patient-derived xenograft (PDX) models from patients with MPT and tested the efficacy of targeting dysregulated pathways in MPT using the PDX model from one MPT.

Results

MPT has unique molecular characteristics when compared to breast cancers of epithelial origin and can be classified into two groups. The PDX model derived from one patient with MPT showed that the mouse epithelial component increased during tumour growth. Moreover, targeted inhibition of platelet-derived growth factor receptor (PDGFR) and phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) by imatinib mesylate and PKI-587 showed in vivo tumour suppression effects.

Conclusions

This study revealed the molecular profiles of MPT that can lead to molecular classification and potential targeted therapy, and suggested that the MPT PDX model can be a useful tool for studying the pathogenesis of fibroepithelial neoplasms and for preclinical drug screening to find new therapeutic strategies for MPT.
Inhibition of branched-chain alpha-keto acid dehydrogenase kinase augments the sensitivity of ovarian and breast cancer cells to paclitaxel
Authors: Ibrahim Suad Lateef , Abed Mohammed Najim , Mohamed Gehad , Price Joshua C. , Abdullah Marwan Ibrahim , Richardson Alan
Published: December 2022

Context

Many cancer patients who initially respond to chemotherapy eventually develop chemoresistance, and to address this, we previously conducted a RNAi screen to identify genes contributing to resistance. One of the hits from the screen was branched-chain α-keto acid dehydrogenase kinase (BCKDK). BCKDK controls the metabolism of branched-chain amino acids (BCAAs) through phosphorylation and inactivation of the branched-chain α-keto acid dehydrogenase complex (BCKDH), thereby inhibiting catabolism of BCAAs.

Methods

We measured the impact on paclitaxel sensitivity of inhibiting BCKDK in ovarian and breast cancer cell lines.

Results

Inhibition of BCKDK using siRNA or two chemical inhibitors (BCKDKi) was synergistic with paclitaxel in both breast and ovarian cancer cells. BCKDKi reduced levels of BCAA and the addition of exogenous BCAA suppressed this synergy. BCKDKi inactivated the mTORC1-Aurora pathway, allowing cells to overcame M-phase arrest induced by paclitaxel. In some cases, cells almost completed cytokinesis, then reverted to a single cell, resulting in multinucleate cells.

Conclusion

BCKDK is an attractive target to augment the sensitivity of cancer cells to paclitaxel.
Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma
Authors: Cao Yu-Fei , Xie Lei , Tong Bei-Bei , Chu Man-Yu , Shi Wen-Qi , Li Xiang , He Jian-Zhong , Wang Shao-Hong , Wu Zhi-Yong , Deng Dan-Xia , Zheng Ya-Qi , Li Zhi-Mao , Xu Xiu-E , Liao Lian-Di , Cheng Yin-Wei , Li Li-Yan , Xu Li-Yan , Li En-Min
Published: December 2022

Anillin (ANLN) is a mitosis-related protein that promotes contractile ring formation and cytokinesis, but its cell cycle-dependent degradation mechanisms in cancer cells remain unclear. Here, we show that high expression of ANLN promotes cytokinesis and proliferation in esophageal squamous cell carcinoma (ESCC) cells and is associated with poor prognosis in ESCC patients. Furthermore, the findings of the study showed that the deubiquitinating enzyme USP10 interacts with ANLN and positively regulates ANLN protein levels. USP10 removes the K11- and K63-linked ubiquitin chains of ANLN through its deubiquitinase activity and prevents ANLN ubiquitin-mediated degradation. Importantly, USP10 promotes contractile ring assembly at the cytokinetic furrow as well as cytokinesis by stabilizing ANLN. Interestingly, USP10 and the E3 ubiquitin ligase APC/C co-activator Cdh1 formed a functional complex with ANLN in a non-competitive manner to balance ANLN protein levels. In addition, the macrolide compound FW-04-806 (F806), a natural compound with potential for treating ESCC, inhibited the mitosis of ESCC cells by targeting USP10 and promoting ANLN degradation. F806 selectively targeted USP10 and inhibited its catalytic activity but did not affect the binding of Cdh1 to ANLN and alters the balance of the USP10-Cdh1-ANLN complex. Additionally, USP10 expression was positively correlated with ANLN level and poor prognosis of ESCC patients. Overall, targeting the USP10-ANLN axis can effectively inhibit ESCC cell-cycle progression.
Optical coherence tomography imaging biomarkers associated with neovascular age-related macular degeneration: a systematic review
Authors: Hanson Rachel L. W. , Airody Archana , Sivaprasad Sobha , Gale Richard P.
Published: December 2022

The aim of this systematic literature review is twofold, (1) detail the impact of retinal biomarkers identifiable via optical coherence tomography (OCT) on disease progression and response to treatment in neovascular age-related macular degeneration (nAMD) and (2) establish which biomarkers are currently identifiable by artificial intelligence (AI) models and the utilisation of this technology. Following the PRISMA guidelines, PubMed was searched for peer-reviewed publications dated between January 2016 and January 2022. Population: Patients diagnosed with nAMD with OCT imaging. Settings: Comparable settings to NHS hospitals. Study designs: Randomised controlled trials, prospective/retrospective cohort studies and review articles. From 228 articles, 130 were full-text reviewed, 50 were removed for falling outside the scope of this review with 10 added from the author’s inventory, resulting in the inclusion of 90 articles. From 9 biomarkers identified; intraretinal fluid (IRF), subretinal fluid, pigment epithelial detachment, subretinal hyperreflective material (SHRM), retinal pigmental epithelial (RPE) atrophy, drusen, outer retinal tabulation (ORT), hyperreflective foci (HF) and retinal thickness, 5 are considered pertinent to nAMD disease progression; IRF, SHRM, drusen, ORT and HF. A number of these biomarkers can be classified using current AI models. Significant retinal biomarkers pertinent to disease activity and progression in nAMD are identifiable via OCT; IRF being the most important in terms of the significant impact on visual outcome. Incorporating AI into ophthalmology practice is a promising advancement towards automated and reproducible analyses of OCT data with the ability to diagnose disease and predict future disease conversion.

Systematic Review Registration: This review has been registered with PROSPERO (registration ID: CRD42021233200).
Exploration of the effects of Coriolis force and thermal radiation on water-based hybrid nanofluid flow over an exponentially stretching plate
Authors: Oke A. S. , Prasannakumara B. C. , Mutuku W. N. , Gowda R. J. Punith , Juma B. A. , Kumar R. Naveen , Bada O. I.
Published: December 2022

Hybrid nanofluids’ enhanced thermophysical properties make them applicable in a plethora of mechanical and engineering applications requiring augmented heat transfer. The present study focuses on a three-dimensional Copper-Aluminium Oxide$$\left( Cu\text{- }Al_{2}O_{3}\right)$$Cu-Al2O3-water based hybrid nanofluid flow within the boundary layer with heat transfer over a rotating exponentially stretching plate, subjected to an inclined magnetic field. The sheet rotates at an angular velocity$$\Omega$$Ωand the angle of inclination of the magnetic field is$$\gamma$$γ. Employing a set of appropriate similarity transformation reduces the governing PDEs to ODEs. The resulting ODEs are solved with the finite difference code with Shooting Technique. Primary velocity increases at large rotation but the secondary velocity reduces as the rotation increases. In addition, the magnetic field is found to oppose the flow and thereby causing a reduction in both the primary and secondary velocities. Increasing the volume fraction reduces the skin friction coefficient and enhances the heat transfer rate.
Impact of leukoaraiosis or blood pressure on clinical outcome, mortality and symptomatic intracerebral hemorrhage after mechanical thrombectomy in acute ischemic stroke
Authors: Göthel-Ezzeiani Annika , Jansen Olav , Austein Friederike , Hofmann Amelie , Berg Daniela , Meyne Johannes , Hensler Johannes
Published: December 2022

We aimed to study the impact of leukoaraiosis (LA) and blood pressure (BP) on clinical outcome, mortality and symptomatic intracerebral hemorrhage (sICH) in acute ischemic stroke (AIS) patients treated with mechanical thrombectomy (MT). We analyzed data retrospectively from 521 patients with anterior large vessel occlusion treated with MT. LA was dichotomized in 0–2 (absent-to-moderate) versus 3–4 (moderate-to-severe) according to the van Swieten scale. Various systolic (SBP) and diastolic (DBP) BP parameters during the first 24 h were collected. Multivariable logistic regressions were performed to identify predictors of a poor 90-day outcome, mortality and sICH. LA was significantly associated with poor outcome (OR 3.2; p < 0.001) and mortality (OR 3.19; p = 0.008), but not sICH (p = 0.19). Higher maximum SBP was significantly associated with poor outcome (OR per 10 mmHg increase = 1.21; p = 0.009) and lower mean DBP was a predictor of mortality (OR per 10 mmHg increase = 0.53; p < 0.001). In the univariate analysis high SBP variability was associated with poor outcome, mortality and sICH, but not in the multivariate model. There was no association between BP and sICH. Severity of LA, SBP variability, high maximum SBP and low DBP are associated with either poor outcome or mortality in AIS patients undergoing MT. However, neither LA nor BP were associated with sICH in our cohort. Thus, mechanisms of the negative impact on outcome remain unclear. Further studies on impact of BP course and its mechanisms and interventions are needed to improve outcome in patients undergoing MT.
Dietary supplementation with Moringa oleifera leaves extract reduces the impacts of sub-lethal fipronil in Nile tilapia, Oreochromis niloticus
Authors: Mahmoud Hemat K. , Farag Mayada R. , Reda Fayiz M. , Alagawany Mahmoud , Abdel-Latif Hany M. R.
Published: December 2022

This study assessed the restorative dietary effects of Moringa oleifera (MO) leaves extract against the negative impacts of sub-lethal fipronil (FIP) toxicity in Nile tilapia. To achieve this purpose, the growth, body composition, haemato-biochemical measurements, serum immunity, and antioxidant condition of Nile tilapia have been examined. Fish were arranged into 6 experimental groups in quadruplicates. Three groups were fed on diets supplemented with 0.0 (reference group), 1.0 (MO1), and 2.0 (MO2) g kg−1 of MO leaf extract. The other three groups were fed on the same MO levels and concomitantly subjected to a sub-lethal FIP concentration (4.2 µg L−1 for 3 h only per day) and defined as FIP, FIP + MO1, and FIP + MO2. The experiment lasted for 8 weeks. Results unveiled that growth parameters were significantly decreased alongside an increased feed conversion ratio in the FIP-intoxicated group. The moisture and crude protein (%) were decreased significantly together with a significant increase of the crude lipids (%) in the fish body of the FIP group. Sub-lethal FIP toxicity induced hypochromic anemia, leukopenia, hypoproteinemia, hypoalbuminemia, hypoglobulinemia, and hepato-renal failure (increased urea and creatinine concentrations, as well as ALT and AST enzymes). Exposure to sub-lethal FIP also induced (a) immunosuppression manifested by a decline in total IgM, complement C3, and lysozyme activities, (b) enzymatic antioxidant misbalance manifested by decreases in SOD and CAT activities, and (c) oxidative stress (declined T-AOC and elevated of MDA concentrations). On the other side, dietary supplementation with MO leaf extract in FIP + MO1 and FIP + MO2 groups noticeably modulated the aforementioned parameters. Therefore, we can conclude that dietary MO could reduce sub-lethal FIP toxicity in Nile tilapia with a possible recommendation for regular prophylaxis supplementation in Nile tilapia diets.
Clinical outcomes following surgical management of deep infiltrating endometriosis
Authors: Leborne Perrine , Huberlant Stephanie , Masia Florent , de Tayrac Renaud , Letouzey Vincent , Allegre Lucie
Published: December 2022

The main aim of the study was to evaluate severe post-operative complications following deep endometriosis surgery in a tertiary referral centre. This is a retrospective cohort study that included women who had surgery for deep infiltrating endometriosis between 1st January 2013 and 31st December 2019. Endometriosis was diagnosed based on clinical, imaging and histological parameters. We evaluated the rates of post-operative complications, potential risk factors for such complications and postoperative pregnancy rates. A total of 165 patients were included in the final analysis. The median follow-up was 63 (25–106) months. Thirty-seven patients (22.42%) had hysterectomy, 60 (36.81%) had ureterolysis and 44 (26.67%) had colorectal surgery. The overall and severe rates of post-operative complications were 16.20% (n = 23) and 2.42% (n = 4) respectively. Of the variables assessed, operative time and age were the only statistically significant risk factor for complications on multivariate analysis. Among women operated on for infertility, 34.5% (n = 20/58) got pregnant following surgery with 30% of these spontaneously. This study demonstrates acceptable overall and severe post-operative complications and pregnancy rates after deep endometriosis surgery. This information should help clinicians when counselling women to enable them making an informed choice about their management.
Development and validation of a new multiplex for upgrading Y-STRs population databases from 12 to 23 markers and its forensic casework application
Authors: Navarro-López Belén , Baeta Miriam , Granizo-Rodríguez Eva , Moreno-López O. , Kleinbielen Tamara , Ferragut Joana Francesca , Picornell Antònia , de Pancorbo Marian M.
Published: December 2022

Y chromosomal short tandem repeats (Y-STRs) are used in forensic investigations as a useful complementary tool to autosomal markers. The ongoing development of new kits with an increasing number of markers makes it necessary to update populations typed in the Y-STR Haplotype Reference Database to reach at least 23 Y-STRs. A novel Y-STR multiplex panel was developed to offer a cost-efficient alternative to update Y-STR haplotypes from 12 to 23 loci. This panel includes the eleven markers, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS576, DYS481, DYS549, DYS533, DYS570 and DYS643, as well as DYS385a/b for traceability purpose. Developmental validation of this panel was conducted following the recommendations of the Scientific Working Group on DNA Analysis Methods (SWGDAM), showing high sensitivity, tolerance to common inhibitors as well as high species specificity. It was efficient for degraded DNA samples and for detection of male mixtures. When applying it for extending the current data of the Ibiza population, both the discrimination capacity and the haplotype diversity increased from 0.5952 to 0.9048 and from 0.9808 to 0.9977, respectively. Together, the study demonstrates the suitability of this panel in forensic casework.
Blast resistance of RC tubular structure under internal ANFO explosion
Authors: Choi Seung-Jai , Lee Tae-Hee , Noor Norhazilan Md. , Kim Jang-Ho Jay
Published: December 2022

The degree of structural damage is significantly more severe when a blast occurs inside than outside of a structure. However, existing designs for RC structures such as reinforced concrete containment vessels (RCCV) do not include design features to protect the structure for internal blast. Therefore, the internal blast resistance capacity of RC structures is evaluated by performing internal blast tests on RC tubular structures. The main objective of the study was to observe and document the basic structural behavior data obtained from internal detonation tests. ANFO explosive charge weights of 15.88, 20.41, 22.68 and 24.95 kg were selected for a charge detonating at a cross section center of the mid-span of the specimen, giving a standoff distance to the inner wall surface of 1000 mm. The data acquisitions include blast pressure, deflection, strain, and crack pattern. When the explosive charge weight increased from 15.88 to 24.95 kg, the peak incident pressure and time duration increased from 0.1718 to 0.3394 MPa and from 5.856 to 5.981 ms, respectively. Then, the test data were used to predict the internal charge weight required to fail a real scale RCCV using simple assumptions and the test data. The results of the study are discussed in detail in the paper.
Development of a machine-learning algorithm to predict in-hospital cardiac arrest for emergency department patients using a nationwide database
Authors: Kim Ji Hoon , Choi Arom , Kim Min Joung , Hyun Heejung , Kim Sunhee , Chang Hyuk-Jae
Published: December 2022

In this retrospective observational study, we aimed to develop a machine-learning model using data obtained at the prehospital stage to predict in-hospital cardiac arrest in the emergency department (ED) of patients transferred via emergency medical services. The dataset was constructed by attaching the prehospital information from the National Fire Agency and hospital factors to data from the National Emergency Department Information System. Machine-learning models were developed using patient variables, with and without hospital factors. We validated model performance and used the SHapley Additive exPlanation model interpretation. In-hospital cardiac arrest occurred in 5431 of the 1,350,693 patients (0.4%). The extreme gradient boosting model showed the best performance with area under receiver operating curve of 0.9267 when incorporating the hospital factor. Oxygen supply, age, oxygen saturation, systolic blood pressure, the number of ED beds, ED occupancy, and pulse rate were the most influential variables, in that order. ED occupancy and in-hospital cardiac arrest occurrence were positively correlated, and the impact of ED occupancy appeared greater in small hospitals. The machine-learning predictive model using the integrated information acquired in the prehospital stage effectively predicted in-hospital cardiac arrest in the ED and can contribute to the efficient operation of emergency medical systems.
Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol
Authors: Zhou Tian , Liang Zhiying , Marchisio Mario Andrea
Published: December 2022

The human estrogen receptor has been used for about thirty years, in the yeast S. cerevisiae, as a component of chimeric transcription factors. Its ligand, β-estradiol, permits to control the protein translocation into the nucleus and, as a consequence, the expression of the gene(s) targeted by the synthetic transcription factor. Activators that are orthogonal to the yeast genome have been realized by fusing the human estrogen receptor to an activation and a DNA-binding domain from bacteria, viruses, or higher eukaryotes. In this work, we optimized the working of a β-estradiol-sensing device—in terms of detection range and maximal output signal—where the human estrogen receptor is flanked by the bacterial protein LexA and either the strong VP64 (from herpes simplex virus) or the weaker B42 (from E. coli) activation domain. We enhanced the biosensor performance by thoroughly engineering both the chimeric activator and the reporter protein expression cassette. In particular, we constructed a synthetic promoter—where transcription is induced by the chimeric activators—based on the core sequence of the yeast CYC1 promoter, by tuning parameters such as the length of the 5′ UTR, the distance between adjacent LexA binding sites (operators), and the spacing between the whole operator region and the main promoter TATA box. We found a configuration that works both as a highly sensitive biosensor and a sharp switch depending on the concentration of the chimeric activator and the strength of its activation domain.
Timing of hypoxia PET/CT imaging after 18F-fluoromisonidazole injection in non-small cell lung cancer patients
Authors: Bourigault Pauline , Skwarski Michael , Macpherson Ruth E. , Higgins Geoff S. , McGowan Daniel R.
Published: December 2022

Positron emission tomography (PET)/computed tomography (CT) using the radiotracer 18F-Fluoromisonidazole (FMISO) has been widely employed to image tumour hypoxia and is of interest to help develop novel hypoxia modifiers and guide radiation treatment planning. Yet, the optimal post-injection (p.i.) timing of hypoxic imaging remains questionable. Therefore, we investigated the correlation between hypoxia-related quantitative values in FMISO-PET acquired at 2 and 4 h p.i. in patients with non-small cell lung cancer (NSCLC). Patients with resectable NSCLC participated in the ATOM clinical trial (NCT02628080) which investigated the hypoxia modifying effects of atovaquone. Two-hour and four-hour FMISO PET/CT images acquired at baseline and pre-surgery visits (n = 58) were compared. Cohort 1 (n = 14) received atovaquone treatment, while cohort 2 (n = 15) did not. Spearman’s rank correlation coefficients (ρ) assessed the relationship between hypoxia-related metrics, including standardised uptake value (SUV), tumour-to-blood ratio (TBR), and tumour hypoxic volume (HV) defined by voxels with TBR ≥ 1.4. As the primary imaging-related trial endpoint used to evaluate the action of atovaquone on tumour hypoxia in patients with NSCLC was change in tumour HV from baseline, this was also assessed in patients (n = 20) with sufficient baseline 2- and 4-h scan HV to reliably measure change (predefined as ≥ 1.5 mL). Tumours were divided into four subregions or distance categories: edge, outer, inner, and centre, using MATLAB. In tumours overall, strong correlation (P < 0.001) was observed for SUVmax ρ = 0.87, SUVmean ρ = 0.91, TBRmax ρ = 0.83 and TBRmean ρ = 0.81 between 2- and 4-h scans. Tumour HV was moderately correlated (P < 0.001) with ρ = 0.69 between 2- and 4-h scans. Yet, in tumour subregions, the correlation of HV decreased from the centre ρ = 0.71 to the edge ρ = 0.45 (P < 0.001). SUV, TBR, and HV values were consistently higher on 4-h scans than on 2-h scans, indicating better tracer-to-background contrast. For instance, for TBRmax, the mean, median, and interquartile range were 1.9, 1.7, and 1.6–2.0 2-h p.i., and 2.6, 2.4, and 2.0–3.0 4-h p.i., respectively. Our results support that FMISO-PET scans should be performed at 4 h p.i. to evaluate tumour hypoxia in NSCLC.

Trial registration: ClinicalTrials.gov, NCT02628080. Registered 11/12/2015, https://clinicaltrials.gov/ct2/show/NCT02628080.
A novel MPPT design based on the seagull optimization algοrithm for phοtovοltaic systems operating under partial shading
Authors: Chalh Abdelilah , chaibi Redouane , Hammoumi Aboubakr El , Motahhir Saad , Ghzizal Abdelaziz El , Al-Dhaifallah Mujahed
Published: December 2022

The use of a maximum power point (MPP) tracking (MPPT) controller is required for photovoltaic (PV) systems to extract maximum power from PV panels. However, under partial shading conditions, the PV cells/panels do not receive uniform insolation due to several power maxima appear on the PV array's P–V characteristic, a global MPP (GMPP) and two or more local MPPs (LMPPs). In this scenerio, conventional MPPT methods, including pertub and observe (P&O) and incremental conductance (INC), fail to differentiate between a GMPP and a LMPP, as they converge on the MPP that makes contact first, which in most cases is one of the LMPPs. This results in considerable energy loss. To address this issue, this paper introduces a new MPPT method based on the Seagull Optimization Algorithm (SOA) to operate PV systems at GMPP with high efficiency. The SOA is a new member of the bio-inspired algorithms. When compared to other evolutionary techniques, it uses fewer operators and modification parameters, which is advantageous when considering the rapid design process. In this paper, the SOA-based MPPT scheme is first proposed and then implemented for an 80 W PV system using the MATLAB/SIMULINK environment. The effectiveness of the SOA based MPPT method is verified by comparing its performance with P& O and PSO (particle swarm optimization) based MPPT methods under different shading scenarios. The results demonstrated that the SOA based MPPT method performs better in terms of tracking accuracy and efficiency.
Association of serum xanthine oxidase levels with hypertension: a study on Bangladeshi adults
Authors: Miah Rakib , Fariha Khandaker Atkia , Sony Sabrina Amita , Ahmed Shamim , Hasan Mahmudul , Mou Ananya Dutta , Barman Zitu , Hasan Akibul , Mohanto Nayan Chandra , Ali Nurshad
Published: December 2022

Xanthine oxidase (XO) is a metalloflavoenzyme associated with the uric acid formation in purine metabolism. Serum XO has been suggested to be associated with liver and kidney dysfunction, diabetes and cardiovascular diseases. However, there is limited information on the relationship between serum XO levels and hypertension. This study aimed to assess the relationship between serum XO levels and hypertension in Bangladeshi adults. In this study, fasting blood samples were collected from 312 participants (225 males and 87 females), aged ≥ 20 years. Serum levels of XO were determined by ELISA and other biochemical parameters including serum uric acid (SUA) were measured by colorimetric methods. Hypertension was defined as SBP ≥ 140 mmHg and/or DBP ≥ 90 mmHg or self-reported recent use of anti-hypertensive medications. Association between serum XO levels and hypertension was evaluated by multinomial logistic regression analysis. The mean level of XO was significantly higher (p < 0.001) in females (5.8 ± 3.2 U/L) than in males (3.9 ± 2.5 U/L). When the participants were divided by blood pressure levels, the mean level of serum XO was significantly higher (p < 0.01) in the hypertensive group (5.0 ± 2.7 U/L) compared to the normotensive control group (4.0 ± 2.7 U/L). An increasing trend for SBP and DBP levels was observed across the XO quartiles (at least p < 0.01 for both cases). A significant positive correlation was found for XO with SBP and DBP (p < 0.01). In regression analysis, the serum levels of XO showed a significant and independent association with hypertension prevalence. In conclusion, the mean level of serum XO was significantly higher in hypertensive individuals and XO was independently associated with the prevalence of hypertension. Our results indicate that XO may have a potential role in the pathophysiology of elevated blood pressure through generating of reactive oxygen species. Further large-scale longitudinal studies are needed to determine the underlying mechanisms between XO and hypertension.
Location management for the supply of PD fluid via large neighborhood search based virus optimization algorithm
Authors: Atthirawong Walailak , Luangpaiboon Pongchanun
Published: December 2022

The facility location problem is extended by a new two-stage zero-one programming system (2S-ZOPS). It is a type of design optimization issue that exists in logistics implementations such as supply chain planning in healthcare or agriculture. Along with concerns regarding PD delivery time manner for connecting logistics centers and customers, recent studies have considered the zero-one location design model. This research discussed a route selection model for the 2S-ZOPS that did not exist in the published studies by taking into account the level of risk associated with physical appearance. The mathematical models were developed in response to a PD supply chain design that occurred in Thailand’s National Health Insurance Program. By combining the virus optimization algorithm (VOA) with a large neighborhood search (LNS), we created a hybrid metaheuristic method for solving the 2S-ZOPS. Experiments with real-world data demonstrated that the hybrid algorithm was efficient in terms of time consumption and solution quality, saving approximately 6% on total costs. The presented practice benefits not only the healthcare industry but also various other businesses.
Rail induced lateral migration of particles across intact co-flowing liquids
Authors: Ziemecka Iwona , de Hemptinne Amaury , Misko Vyacheslav R. , Briet Matthieu , Gelin Pierre , Bihi Ilyesse , Maes Dominique , De Malsche Wim
Published: December 2022

This paper presents a rail guided method to apply a Layer-by-Layer (LbL) coating on particles in a microfluidic device. The passive microfluidic approach allows handling suspensions of particles to be coated in the system. The trajectory of the particles is controlled using engraved rails, inducing lateral movement of particles while keeping the axially oriented liquid flow (and the interface of different liquids) undisturbed. The depth and angle of the rails together with the liquid velocity were studied to determine a workable geometry of the device. A discontinuous LbL coating procedure was converted into one continuous process, demonstrating that the chip can perform seven consecutive steps normally conducted in batch operation, further easily extendable to larger cycle numbers. Coating of the particles with two bilayers was confirmed by fluorescence microscopy.
Vulcanimicrobium alpinus gen. nov. sp. nov., the first cultivated representative of the candidate phylum “Eremiobacterota”, is a metabolically versatile aerobic anoxygenic phototroph
Authors: Yabe Shuhei , Muto Kiyoaki , Abe Keietsu , Yokota Akira , Staudigel Hubert , Tebo Bradley M
Published: December 2022

The previously uncultured phylum “Candidatus Eremiobacterota” is globally distributed and often abundant in oligotrophic environments. Although it includes lineages with the genetic potential for photosynthesis, one of the most important metabolic pathways on Earth, the absence of pure cultures has limited further insights into its ecological and physiological traits. We report the first successful isolation of a “Ca. Eremiobacterota” strain from a fumarolic ice cave on Mt. Erebus volcano (Antarctica). Polyphasic analysis revealed that this organism is an aerobic anoxygenic photoheterotrophic bacterium with a unique lifestyle, including bacteriochlorophyll a production, CO2 fixation, a high CO2 requirement, and phototactic motility using type IV-pili, all of which are highly adapted to polar and fumarolic environments. The cells are rods or filaments with a vesicular type intracytoplasmic membrane system. The genome encodes novel anoxygenic Type II photochemical reaction centers and bacteriochlorophyll synthesis proteins, forming a deeply branched monophyletic clade distinct from known phototrophs. The first cultured strain of the eighth phototrophic bacterial phylum which we name Vulcanimicrobium alpinus gen. nov., sp. nov. advances our understanding of ecology and evolution of photosynthesis.
An ion mobility-mass spectrometry study of copper-metallothionein-2A: binding sites and stabilities of Cu-MT and mixed metal Cu–Ag and Cu–Cd complexes
Authors: Fan Liqi , Russell David H.
Published: December 2022

Cu(i) binds to human metallothionein MT2 to form stable Cu6- and Cu10-MT complexes that exhibit high gas-phase stabilities.
Evaluation of grade and invasiveness of bladder urothelial carcinoma using infrared imaging and machine learning
Authors: Kujdowicz Monika , Perez-Guaita David , Chlosta Piotr , Okon Krzysztof , Malek Kamilla
Published: December 2022

We combined infrared (IR) microscopy of bladder sections with machine learning (partial least squares-discriminant analysis) to provide an automated methodology for the prediction of the presence of cancer, invasiveness and grade.
Isolation and quantification of synthetic cannabinoid receptor agonists in human urine using membrane-assisted solvent extraction followed by liquid chromatography-tandem mass spectrometry
Authors: Suárez-Oubiña Cristian , Álvarez-Freire Iván , Cabarcos Pamela , Bermejo Ana María , Bermejo-Barrera Pilar , Moreda-Piñeiro Antonio
Published: December 2022

Membrane assisted solvent extraction (MASE) is an appealing environmental-friendly pre-treatment for isolating trace targets. MASE applicability in the forensic laboratory is demonstrated by assessing synthetic cannabinoid receptor agonists in urine.
QbD-based fabrication of transferrin-anchored nanocarriers for targeted drug delivery to macrophages and colon cells for mucosal inflammation healing
Authors: Zeeshan Mahira , Ain Qurat Ul , Sunny Ahad , Raza Faisal , Mohsin Muhammad , Khan Salman , Weigmann Benno , Ali Hussain
Published: December 2022

QbD-designed PLGA/transferrin nanocarriers (P/tfr NCs) specifically deliver the drug to the colon by targeting colon epithelial cells and macrophagesviaoverexpressed surface transferrin receptor (TFR-1) to ameliorate inflammationviaoral route.
Facile iodine-promoted synthesis of bis(1-imidazo[1,5-a]pyridyl)arylmethanes and exploration of applications
Authors: Phuc Ban Van , Nguyen Nina Thi , Van Nguyen Thi Hong , Nguyen Thanh Luan , Nguyen Van Ha , Tran Cong Minh , Nguyen Hien , Nguyen Minh Tho , Hung Tran Quang , Dang Tuan Thanh
Published: December 2022

I2-promoted synthesis of BIMPYs.
Mixed-valent palladium(iv/ii)-oxoanion, [PdIVO6PdII6((CH3)2AsO2)6]2−
Authors: Ma Xiang , Bhattacharya Saurav , Nisar Talha , Müller Anja B. , Wagner Veit , Kuhnert Nikolai , Kortz Ulrich
Published: December 2022

We report on the synthesis and structural characterizaton of the first example of a PdIV-containing polyoxopalladate(ii).
Pd-catalyzed access to mono- and di-fluoroallylic amines from primary anilines
Authors: Wang Xingben , Patureau Frederic W.
Published: December 2022

The Pd-catalyzed highly selective synthesis of mono- and di-2-fluoroallylic amines is herein described from gem-difluorocyclopropanes and simple primary anilines.
Endogenous G-quadruplex-forming RNAs inhibit the activity of SARS-CoV-2 RNA polymerase
Authors: Endoh Tamaki , Takahashi Shuntaro , Sugimoto Naoki
Published: December 2022

Guanine-rich RNA sequences selected from endogenous transcriptome library based on the interaction with RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 could inhibit RNA polymerization catalyzed by RdRp depending of the formation of G-quadruplex.
Real-time visualization of the fluctuations in HOBr with AIE fluorescent probes during myocardial ischemia-reperfusion injury
Authors: Zhang Jian , Xie Yingying , Ma Jushuai , Liu Kaiqiang , Ding Yunshu , Li Yong , Jiao Xiaoyun , Xie Xilei , Wang Xu , Tang Bo
Published: December 2022

Two AIE fluorescent probes (QM-S and QM-Se) have been developed to monitor the increased intracellular HOBr in cardiomyocytes during MIRI. It was also indicated that MIRI might be alleviated by combating oxidative stress as well as inhibiting inflammation and ferroptosis.
CuII-mediated DNA base pairing of a triazole-4-carboxylate nucleoside prepared by click chemistry
Authors: Hu Lingyun , Takezawa Yusuke , Shionoya Mitsuhiko
Published: December 2022

Triazole-4-carboxylate nucleosides synthesized by the click reaction form a CuII-mediated artificial base pair within DNA duplexes and stabilize the duplex in a CuII-specific manner.
Metal-free FRET macrocycles of perylenediimide and aza-BODIPY for multifunctional sensing
Authors: Rani Kavita , Sengupta Sanchita
Published: December 2022

Multichromophoric metal-free FRET macrocycles M1 [1+1] and M2 [2+2] composed of PDI and ABDP have been synthesized and showed distinct stimuli responsiveness towards temperature and redox potential variation.
Synthesis, structure and magnetic properties of two bis(oxalato)cuprate(ii) salts with pyridinium type counter ions
Authors: Dazem Cyrielle L. F. , Inge A. Ken , Luneau Dominique , Öhrström Lars , Nenwa Justin
Published: December 2022

In the Cu(ii) oxalate salts of (C5H7N2O)2[Cu(C2O4)2] 1 and C13H16N2[Cu(C2O4)2] 2, the oxalate adopts different coordination modes: the common bidentate chelating oxalate in 1 and the relatively scarce μ-oxalato-κ3O1,O2:O1′ in 2.
Partner effect in accelerating pincer-co catalyzed nitrile hydroboration reactions
Authors: Ma Yumiao , Hussein Aqeel A.
Published: December 2022

A partner strategy of using an additive to reversibly bind the ligand of a catalyst is proposed by DFT calculations, showing an improvement in the catalytic reactivity without ligand engineering.
Excited-state and charge-carrier dynamics in binary conjugated polymer dots towards efficient photocatalytic hydrogen evolution
Authors: Liu Aijie , Wang Sicong , Song Hongwei , Liu Yawen , Gedda Lars , Edwards Katarina , Hammarström Leif , Tian Haining
Published: December 2022

Tuning the ratio of the donor and acceptor in heterojunction Pdots can greatly influence the charge and energy transfer pathways and dynamics, therefore exhibiting a significant impact on photocatalytic hydrogen production.
Structural and dynamical insights into SilE silver binding from combined analytical probes
Authors: Monneau Yoan , Arrault Cyrielle , Duroux Coraline , Martin Marie , Chirot Fabien , Mac Aleese Luke , Girod Marion , Comby-Zerbino Clothilde , Hagège Agnès , Walker Olivier , Hologne Maggy
Published: December 2022

Structural and dynamical insights into silver-bound SilE have been revealed by an ensemble of analytical and biophysical techniques.
Energy dispersive anti-anharmonic effect in a Fano intervened semiconductor: revealed through temperature and wavelength-dependent Raman scattering
Authors: Rani Chanchal , Kandpal Suchita , Ghosh Tanushree , Bansal Love , Tanwar Manushree , Kumar Rajesh
Published: December 2022

Dissociation energy taken to break the electron-phonon bound state (Fano-interferons) varies with excitation photon energy. The Fano interaction weakens by decreasing wavelength or increasing temperature as seen in the Raman thermal map.
Unveiling the correlation between structural and magnetic ordering in nano Co1−xNixTeO4
Authors: Patel Akhilesh Kumar , Samatham S. Shanmukharao , Rani Ekta , Suresh K. G. , Singh Harishchandra
Published: December 2022

Nanomaterials with unique structures and exotic magnetic phenomena are always intriguing; however, the direct correlation of structural and magnetic ordering up to a few nanometers remains critical.
Direct reuse of aluminium and copper current collectors from spent lithium-ion batteries
Authors: Zhu Pengcheng , Driscoll Elizabeth H. , Dong Bo , Sommerville Roberto , Zorin Anton , Slater Peter R. , Kendrick Emma
Published: December 2022

Direct reuse of current collectors can not only effectively tackle LIB waste issues but also skip long conventional recycling processes, bringing environmental and economic benefits.
Cells function as a ternary logic gate to decide migration direction under integrated chemical and fluidic cues
Authors: Moon Hye-ran , Saha Soutick , Mugler Andrew , Han Bumsoo
Published: December 2022

A reverse-engineered systems biology framework of directed cell migration in which cells function as a ternary logic gate to decode entangled chemical and fluidic cues to determine migration direction.
Enhanced two-step two-frequency upconversion luminescence in a core/shell/shell nanostructure
Authors: Han Dongcheng , Yang Shizhi , Zhao Qiang , Zhang Liangliang , Deng Yan
Published: December 2022

The designed UCNPs were firstly applied in enhancing TSTF UCL. The amplified UCL was attributed to reduction of surface quenching and improvement of 1550 nm absorption. The design provided a novel strategy for studying the mechanism of TSTF UCL.
A polydopamine modified resin loaded nZVI composite for hexavalent chromium removal from aqueous solution
Authors: Zhong Dengjie , Gao Wei , Xu Yunlan
Published: December 2022

PDA/nZVI/D201 was successfully prepared, and it obtained 184.9 mg g−1 of maximum Cr(vi) adsorption capacity due to the fact that carrier D201 and surface coating PDA improved the dispersibility and the oxidation resistance of nZVI, respectively.
Exploring the electrochemical stability mechanism of a SnS2-based composite in dimethoxyethane electrolytes for potassium ion batteries
Authors: Zhang Jizu , Chen Sibo , Huang Zuhang , Zhang Wanggang , Yuan Zhicong , Liu Yiming , Mai Wenjie , Li Jinliang
Published: December 2022

Dimethoxyethane-based electrolytes with high K salt concentration achieves stable cycling of SnS2-containing composite in potassium-ion batteries.
Correction: Optical properties and photoactivity of carbon nanodots synthesized from olive solid wastes at different carbonization temperatures
Authors: Sawalha Shadi , Assali Mohyeddin , Nasasrah Ameerah , Salman Maha , Nasasrah Majd , Jitan Madleen , Hilal Hikmat S. , Zyoud Ahed
Published: December 2022

Correction for ‘Optical properties and photoactivity of carbon nanodots synthesized from olive solid wastes at different carbonization temperatures’ by Shadi Sawalha et al., RSC Adv., 2022, 12, 4490–4500, https://doi.org/10.1039/D1RA09273A.
Correction: Solution processed high performance perovskite solar cells based on a silver nanowire-titanium dioxide hybrid top electrode
Authors: Mahmood Khalid , Akhtar Hafiz Husnain , Qutab Haji Ghulam , Ramzan Naveed , Sharif Rabia , Rehman Abdul , Khalid Arshi , Mehran Muhammad Taqi
Published: December 2022

Correction for ‘Solution processed high performance perovskite solar cells based on a silver nanowire-titanium dioxide hybrid top electrode’ by Khalid Mahmood et al., RSC Adv., 2022, 12, 35350–35357, https://doi.org/10.1039/D2RA06778A.
Diagnosis of cancer using carbon nanomaterial-based biosensors
Authors: Das Suman , Saha Bijay , Tiwari Manisha , Tiwari Dhermendra K.
Published: December 2022

Basic principle of sensing technique of various carbon nanomaterial-based biosensors.
A monocarboxylic acid induction strategy to prepare tough and thermo-reversible poly(vinyl alcohol) physical gels with high transparency
Authors: Zhong Weifeng , Song Yufang , Yang Shuai , Gong Lihao , Shi Dongjian , Dong Weifu , Zhang Hongji
Published: December 2022

Herein, we report a novel monocarboxylic acid induction strategy to construct tough and thermo-reversible PVA physical gels with high transparency.
Gradient hydrogel actuator with fast response and self-recovery in air
Authors: Liu E. , Xia Xuehuan , Chen Qiuyue , Xu Shimei
Published: December 2022

A gradient HA/PNIPAM hydrogel actuator prepared by an asymmetric mold method exhibits fast response above LCST in air and unusual self-recovery without the need of further temperature stimuli.
Controlling dielectric properties of Nb + X (X = Al, Ga, In) co-doped and Nb-doped rutile-type TiO2 single crystals
Authors: Kakimoto Shota , Hashimoto Yujiro , Kuwano Taro , Kimura Koji , Hayashi Kouichi , Hagiwara Manabu , Deguchi Kazuhiko , Taniguchi Hiroki
Published: December 2022

Dielectric properties of Nb-doped TiO2 single crystals measured before and after the special post-annealing with Ga2O3 embedding powder, shedding light on local structure engineering for permittivity boosting in dielectric materials.
Dysregulated haemostasis in thrombo-inflammatory disease
Authors: Klavina Paula A. , Leon Gemma , Curtis Annie M. , Preston Roger J.S.
Published: December 2022

Inflammatory disease is often associated with an increased incidence of venous thromboembolism in affected patients, although in most instances, the mechanistic basis for this increased thrombogenicity remains poorly understood. Acute infection, as exemplified by sepsis, malaria and most recently, COVID-19, drives ‘immunothrombosis’, where the immune defence response to capture and neutralise invading pathogens causes concurrent activation of deleterious prothrombotic cellular and biological responses. Moreover, dysregulated innate and adaptive immune responses in patients with chronic inflammatory conditions, such as inflammatory bowel disease, allergies, and neurodegenerative disorders, are now recognised to occur in parallel with activation of coagulation. In this review, we describe the detailed cellular and biochemical mechanisms that cause inflammation-driven haemostatic dysregulation, including aberrant contact pathway activation, increased tissue factor activity and release, innate immune cell activation and programmed cell death, and T cell-mediated changes in thrombus resolution. In addition, we consider how lifestyle changes increasingly associated with modern life, such as circadian rhythm disruption, chronic stress and old age, are increasingly implicated in unbalancing haemostasis. Finally, we describe the emergence of potential therapies with broad-ranging immunothrombotic functions, and how drug development in this area is challenged by our nascent understanding of the key molecular and cellular parameters that control the shared nodes of proinflammatory and procoagulant pathways. Despite the increasing recognition and understanding of the prothrombotic nature of inflammatory disease, significant challenges remain in effectively managing affected patients, and new therapeutic approaches to curtail the key pathogenic steps in immune response-driven thrombosis are urgently required.
Unravelling the microscopic characteristics of intrinsically disordered proteins upon liquid–liquid phase separation
Authors: Wu Si , Wen Jitao , Perrett Sarah
Published: December 2022

Biomolecular condensate formation via liquid–liquid phase separation (LLPS) has emerged as a ubiquitous mechanism underlying the spatiotemporal organization of biomolecules in the cell. These membraneless condensates form and disperse dynamically in response to environmental stimuli. Growing evidence indicates that the liquid-like condensates not only play functional physiological roles but are also implicated in a wide range of human diseases. As a major component of biomolecular condensates, intrinsically disordered proteins (IDPs) are intimately involved in the LLPS process. During the last decade, great efforts have been made on the macroscopic characterization of the physicochemical properties and biological functions of liquid condensates both in vitro and in the cellular context. However, characterization of the conformations and interactions at the molecular level within phase-separated condensates is still at an early stage. In the present review, we summarize recent biophysical studies investigating the intramolecular conformational changes of IDPs upon LLPS and the intermolecular clustering of proteins undergoing LLPS, with a particular focus on single-molecule fluorescence detection. We also discuss how these microscopic features are linked to the macroscopic phase transitions that are relevant to the physiological and pathological roles of the condensates.
Retracted: Using Markov predictions for handling and allocating task to virtual machines in clouds data centres
Authors: Zavieh Hadi , Javadpour Amir , Li Yuan , Ja'fari Forough , Sangaiah Arun Kumar , Zhang Weizhe , Nasseri Seyed Hadi
Published: December 2022

The above article from IET Communications, published online on 16 December 2022 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the Interim Editor‐in‐Chief, Jian Ren, the Institution of Engineering and Technology (the IET) and John Wiley and Sons Ltd. This article was published as part of a Guest Edited special issue. Following an investigation, the IET and the journal have determined that the article was not reviewed in line with the journal's peer review standards and there is evidence that the peer review process of the special issue underwent systematic manipulation. Accordingly, we cannot vouch for the integrity or reliability of the content. As such we have taken the decision to retract the article. The authors have been informed of the decision to retract.