bluuebunny/crossref_metadata_2025 huggingface dataset:
The Bilateral Precuneus as a Potential Neuroimaging Biomarker for Right Temporal Lobe Epilepsy: A Support Vector Machine Analysis
Authors: Huang Chunyan , Zhou Yang , Zhong Yi , Wang Xi , Zhang Yunhua
Published: June 2022

Background and Objective

While evidence has demonstrated that the default-mode network (DMN) plays a key role in the broad-scale cognitive problems that occur in right temporal lobe epilepsy (rTLE), little is known about alterations in the network homogeneity (NH) of the DMN in TLE. In this study, we used the NH method to investigate the NH of the DMN in TLE at rest, and an support vector machine (SVM) method for the diagnosis of rTLE.

Methods

A total of 43 rTLE cases and 42 healthy controls (HCs) underwent resting-state functional magnetic resonance imaging (rs-fMRI). Imaging data were analyzed with the NH and SVM methods.

Results

rTLE patients have a decreased NH in the right inferior temporal gyrus (ITG) and left middle temporal gyrus (MTG), but increased NH in the bilateral precuneus (PCu) and right inferior parietal lobe (IPL), compared with HCs. We found that rTLE had a longer performance reaction time (RT). No significant correlation was found between abnormal NH values and clinical variables of the patients. The SVM results showed that increased NH in the bilateral PCu as a diagnostic biomarker distinguished rTLE from HCs with an accuracy of 74.12% (63/85), a sensitivity 72.01% (31/43), and a specificity 72.81% (31/42).

Conclusion

These findings suggest that abnormal NH of the DMN exists in rTLE, and highlights the significance of the DMN in the pathophysiology of cognitive problems occurring in rTLE, and the bilateral PCu as a neuroimaging diagnostic biomarker for rTLE.
Epidemiology of Constipation in Elderly People in Parts of China: A Multicenter Study
Authors: Du Xiaoshan , Liu Shuai , Jia Peifei , Wang Xiaodan , Gan Jinghuan , Hu Wenzheng , Zhu Han , Song Yehua , Niu Jianping , Ji Yong
Published: June 2022

Purpose

To investigate the epidemiological characteristics of constipation in people aged 65 years and older in several regions of China.

Patients and Methods

A cross-sectional study based on a cluster sampling design was conducted in four cities of China: Tianjin, Xiamen, Cangzhou and Harbin. A total of 5,222 cases (age ≥ 65 years) were recruited, and the survey was conducted via centralized and household questionnaires that included the following: basic demographic characteristics such as sex, age, education, marital status, living status and occupation; social activities; duration of sleep at night; duration of menstruation and delivery times (in females); and if the participant had constipation symptoms, the severity of constipation. Constipation was diagnosed according to the Rome IV criteria.

Results

Of the 5,222 participants, 919 were diagnosed with constipation. The prevalence of constipation was 17.60% in elderly people ≥65 years old. Prevalence increased with age and was significantly higher in females than males (P < 0.05). Prevalence was lower in the manual compared to the non-manual worker group, and significantly increased with decreasing duration of night sleep (P < 0.05). Older age, female sex and shorter sleep duration at night were risk factors for constipation in elderly people.

Conclusion

The prevalence of constipation in the elderly people in four cities of China was 17.60%, and was significantly affected by age, sex and sleep duration at night.
Sources of Conflict and Prevention Proposals in User Violence Toward Primary Care Staff: A Qualitative Study of the Perception of Professionals
Authors: Pina David , Peñalver-Monteagudo Carmen María , Ruiz-Hernández José Antonio , Rabadán-García José Antonio , López-Ros Paloma , Martínez-Jarreta Begoña
Published: June 2022

Background

Some studies indicate that at least one in four cases of workplace violence occurs in the health sector, with a higher incidence in Emergency Departments, Mental Health Services or Primary Care. Unlike other professional groups, healthcare workers perceive this type of behavior mainly from users or patients. This is the reason why both the detection of conflict between users and professionals and the ways to face and reduce these conflicts has been and is one of the main fields of study in this population. The aim of this study was to delve into the sources of conflict between users and professionals in Primary Care from the perspective of the professionals themselves. In addition, the aim was to explore the proposals for intervention/prevention of this conflict that the professionals perceived as necessary to improve the work environment.

Methods

This study uses qualitative methodology conducting 8 focus groups with professionals related to Primary Health Care. The final sample was composed of 44 workers who were part of the regional management, labor unions, area coordinators, center coordinators and representatives of the professional groups of these centers (medicine, nursing and administration). Thematic analysis was used to extract topics and subtopics.

Results

The results are divided into areas of conflict and intervention proposals. The professionals detect a lack of training or education in themselves, absence of functional multidisciplinary teams or competencies to improve the patient-professional relationship, among others. To address these shortcomings, they propose the creation of protocols for action in the face of aggression, the formation of spaces and channels of communication both among the center's own workers and between them and other organizations (e.g., hospitals), fostering a positive relationship with the user community and ongoing training in various topics such as self-safety, management of emotions, empathy or interpersonal communication.

Conclusions

This study allows to highlight specific areas of user-professional conflict in Primary Care. Furthermore, the inclusion of intervention proposals by the professionals allows to propose starting points for the development of complete plans.
Assessing Vaccination Prioritization Strategies for COVID-19 in South Africa Based on Age-Specific Compartment Model
Authors: Zuo Chao , Meng Zeyang , Zhu Fenping , Zheng Yuzhi , Ling Yuting
Published: June 2022

The vaccines are considered to be important for the prevention and control of coronavirus disease 2019 (COVID-19). However, considering the limited vaccine supply within an extended period of time in many countries where COVID-19 vaccine booster shot are taken and new vaccines are developed to suppress the mutation of virus, designing an effective vaccination strategy is extremely important to reduce the number of deaths and infections. Then, the simulations were implemented to study the relative reduction in morbidity and mortality of vaccine allocation strategies by using the proposed model and actual South Africa's epidemiological data. Our results indicated that in light of South Africa's demographics, vaccinating older age groups (>60 years) largely reduced the cumulative deaths and the “0–20 first” strategy was the most effective way to reduce confirmed cases. In addition, “21–30 first” and “31–40 first” strategies have also had a positive effect. Partial vaccination resulted in lower numbers of infections and deaths under different control measures compared with full vaccination in low-income countries. In addition, we analyzed the sensitivity of daily testing volume and infection rate, which are critical to optimize vaccine allocation. However, comprehensive reduction in infections was mainly affected by the vaccine proportion of the target age group. An increase in the proportion of vaccines given priority to “0–20” groups always had a favorable effect, and the prioritizing vaccine allocation among the “60+” age group with 60% of the total amount of vaccine consistently resulted in the greatest reduction in deaths. Meanwhile, we observed a significant distinction in the effect of COVID-19 vaccine allocation policies under varying priority strategies on relative reductions in the effective reproduction number. Our results could help evaluate to control measures performance and the improvement of vaccine allocation strategy for COVID-19 epidemic.
Views of Pharmacists and Government Representatives Toward the Pilot Chief Pharmacist System in Chinese Hospitals: A Multicenter Exploratory Qualitative Study
Authors: Yang Ruomeng , Li Qian , Hayat Khezar , Zhai Panpan , Liu Wenchen , Chen Chen , Saeed Amna , Chang Jie , Li Pengchao , Du Qianqian , Xu Sen , Wen Jun , Fang Yu
Published: June 2022

Background

In China, the pharmacy departments of most hospitals have changed their main focus from drug procurement and distribution to providing pharmaceutical care services. Various regions of China have successively implemented the pilot Chief Pharmacist System (CPS) to help improve pharmaceutical care services and rational drug use in hospitals. This study was designed to explore the perspectives of pharmacists and government officials on CPS, including the advantages and barriers to the successful implementation of CPS.

Methods

A qualitative study, based on semi-structured interviews, was conducted from October 1, 2018 to March 1, 2019. The interview data were gathered from 18 pharmacy staff and government representatives working in five distinct regions of China using purposive sampling. A thematic analysis approach and NVivo version 12 Plus was utilized to code and analysis of all interviews.

Results

Five broad themes were identified: the role of the chief pharmacist; their attitudes toward the CPS; the advantages and results of the CPS; the barriers toward CPS; and their suggestions toward CPS. Most of the participants believed that the chief pharmacist played a vital role in a hospital. Under CPS, the hospital pharmacy department pays more attention to prescription review, medication monitoring, and pharmaceutical consultation. However, an insufficient number of pharmacy personnel, unclear authority, and inadequate salaries were the main barriers to the implementation of the CPS.

Conclusion

The attitudes of most of the participants were found to be positive toward CPS in China. The CPS can enhance the prestige of the hospital pharmacy department, improve the quality of hospital pharmaceutical care services, and promote rational drug use. Nevertheless, certain barriers highlighted in this study should be addressed promptly.
Regular Exercise Decreases the Risk of Osteoporosis in Postmenopausal Women
Authors: Chang Chu-Fen , Lee Jia-In , Huang Shu-Pin , Geng Jiun-Hung , Chen Szu-Chia
Published: June 2022

Regular exercise can regulate bone maintenance and improve bone health. However, large-scale epidemiological studies on the association between regular exercise and incident osteoporosis in menopausal women are still lacking. We aimed to examine the relationship between exercise and the risk of osteoporosis in menopausal women. In cross-sectional analysis, we enrolled 30,046 postmenopausal women with available information from the database of the Taiwan Biobank (TWB). We divided them into two groups according to their status of regular exercise, i.e., no exercise and regular exercise groups. A t-score of −2.5 or more standard deviations (SDs) below that of a young adult was defined as osteoporosis. Logistic regression after adjusting for confounding factors was used to analyze the association between regular exercise and the prevalence of osteoporosis. Furthermore, the risk of incident osteoporosis development was analyzed in a longitudinal cohort of 6,785 postmenopausal women without osteoporosis at baseline using a Kaplan-Meier analysis and a log-rank test. The mean age of subjects in the cross-sectional cohort was 59 years old. Fifty-six percent of them were exercising regularly. Osteoporosis was observed in 1,886 (14.2%) and 2,254 (13.4%) participants in the no exercise and regular exercise groups. Lower risk of osteoporosis was noted in postmenopausal women with regular exercise when compared with those without regular exercise [odds ratio (OR), 0.76; 95% confidence interval (95% CI), 0.71–0.81]. In the longitudinal cohort, incident osteoporosis was found in 430 (10.5%) women with regular exercise and 299 (11.2%) women without exercise during a mean follow-up of 45 months. Cox regression analysis revealed that the risk for incident osteoporosis was lower in postmenopausal women with regular exercise than those without exercise [hazard ratio (HR), 0.83; 95% CI, 0.71–0.97]. Our study suggests that regular exercise is associated with a reduced risk of osteoporosis in postmenopausal women and strengthens the importance of exercise for the prevention of osteoporosis.
The Association Between Cognitive Impairment and Subsequent Falls Among Older Adults: Evidence From the China Health and Retirement Longitudinal Study
Authors: Zhou Rong , Li Jiayu , Chen Meiling
Published: June 2022

Introduction

Previous studies have suggested that cognitive impairment is associated with falls in older adults. However, the consistency of results among different subgroups defined by multiple characteristics of the elderly has not yet been tested. Additionally, results are inconsistent regarding the effects of different cognitive domains on falls. Therefore, this study sought to use representative data from a nationwide study to better understand the longitudinal association between cognitive impairment and subsequent falls in older adults.

Methods

The current study was conducted based on the China Health and Retirement Longitudinal Study (CHARLS) data of respondents aged ≥60 years in 2015 and the fall data in 2018. The respondents were divided into subgroups according to different demographic characteristics. Multiple logistic regression analysis was conducted to adjust for various confounding factors and evaluate the association between cognitive impairment and falls.

Results

Of the 5,110 respondents included in this study, 1,093 (21.39%) had falls within the last 2 years. A significant association was found between cognitive impairment and subsequent falls (OR = 0.97, 95% CI 0.95–0.99, P = 0.001) after adjusting for all of the covariates related to falls. Analysis of different cognitive domains showed that orientation (OR = 0.94, 95% CI 0.90–0.99, P = 0.013) and memory (OR = 0.93, 95% CI 0.90–0.97, P = 0.001) were significantly associated with falls. In subgroup analysis, the ORs of people aged 60–74 years, male, with lower education level were 0.97 (95% CI 0.95–0.99, P = 0.008), 0.96 (95% CI 0.93–0.98, P = 0.001), and 0.97 (95% CI 0.95–0.99, P = 0.001), respectively, suggesting that the associations were also statistically significant in these subgroups. There was also a significant association both in urban (OR = 0.97, 95% CI 0.95–0.99, P = 0.001) and in rural residents (OR = 0.97, 95% CI 0.95–0.99, P = 0.003).

Conclusions

Our results suggest that the associations between cognition and falls vary depending on the different demographic characteristics of older adults. These findings may be useful for designing more accurate identification and intervention for the fall risk for specific high-risk groups.
Physical Activity Impacts of an Activity-Friendly Community: A Natural Experiment Study Protocol
Authors: Zhu Xuemei , Ory Marcia G. , Xu Minjie , Towne Samuel D. , Lu Zhipeng , Hammond Tracy , Sang Huiyan , Lightfoot J. Timothy , McKyer E. Lisako J. , Lee Hanwool , Sherman Ledric D. , Lee Chanam
Published: June 2022

Background

Stakeholders from multiple sectors are increasingly aware of the critical need for identifying sustainable interventions that promote healthy lifestyle behaviors. Activity-friendly communities (AFCs) have been known to provide opportunities for engaging in physical activity (PA) across the life course, which is a key to healthy living and healthy aging.

Purpose

Our purpose is to describe the study protocol developed for a research project that examines: (a) the short- and long-term changes in total levels and spatial and temporal patterns of PA after individuals move from non-AFCs to an AFC; and (b) what built and natural environmental factors lead to changes in PA resulting from such a move, either directly or indirectly (e.g., by affecting psychosocial factors related to PA).

Methods

This protocol is for a longitudinal, case-comparison study utilizing a unique natural experiment opportunity in Austin, Texas, USA. Case participants were those adults who moved from non-AFCs to an AFC. Matching comparison participants were residents from similar non-AFCs who did not move during the study period. Recruitment venues included local businesses, social and print media, community events, and individual referrals. Objectively measured moderate-to-vigorous PA and associated spatial and temporal patterns served as the key outcomes of interest. Independent (e.g., physical environments), confounding (e.g., demographic factors), and mediating variables (e.g., psychosocial factors) were captured using a combination of objective (e.g., GIS, GPS, Tanita scale) and subjective measures (e.g., survey, travel diary). Statistical analyses will be conducted using multiple methods, including difference-in-differences models, repeated-measures linear mixed models, hierarchical marked space-time Poisson point pattern analysis, and hierarchical linear mixed models.

Conclusion

Natural experiment studies help investigate causal relationships between health and place. However, multiple challenges associated with participant recruitment, extensive and extended data collection activities, and unpredictable intervention schedules have discouraged many researchers from implementing such studies in community-based populations. This detailed study protocol will inform the execution of future studies to explore how AFCs impact population health across the life course.
Assisting Forearm Function in Children With Movement Disorders via A Soft Wearable Robot With Equilibrium-Point Control
Authors: Realmuto Jonathan , Sanger Terence D.
Published: June 2022

Wearable robots are envisioned to amplify the independence of people with movement impairments by providing daily physical assistance. For portable, comfortable, and safe devices, soft pneumatic-based robots are emerging as a potential solution. However, due to the inherent complexities, including compliance and nonlinear mechanical behavior, feedback control for facilitating human–robot interaction remains a challenge. Herein, we present the design, fabrication, and control architecture of a soft wearable robot that assists in supination and pronation of the forearm. The soft wearable robot integrates an antagonistic pair of pneumatic-based helical actuators to provide active pronation and supination torques. Our main contribution is a bio-inspired equilibrium-point control scheme for integrating proprioceptive feedback and exteroceptive input (e.g., the user’s muscle activation signals) directly with the on/off valve behavior of the soft pneumatic actuators. The proposed human–robot controller is directly inspired by the equilibrium-point hypothesis of motor control, which suggests that voluntary movements arise through shifts in the equilibrium state of the antagonistic muscle pair spanning a joint. We hypothesized that the proposed method would reduce the required effort during dynamic manipulation without affecting the error. In order to evaluate our proposed method, we recruited seven pediatric participants with movement disorders to perform two dynamic interaction tasks with a haptic manipulandum. Each task required the participant to track a sinusoidal trajectory while the haptic manipulandum behaved as a Spring-Dominate system or Inertia-Dominate system. Our results reveal that the soft wearable robot, when active, reduced user effort on average by 14%. This work demonstrates the practical implementation of an equilibrium-point volitional controller for wearable robots and provides a foundational path toward versatile, low-cost, and soft wearable robots.
Towards Autonomous Robotic Biopsy—Design, Modeling and Control of a Robot for Needle Insertion of a Commercial Full Core Biopsy Instrument
Authors: Sajadi Seyed MohammadReza , Karbasi Seyed Mojtaba , Brun Henrik , Tørresen Jim , Elle Ole Jacob , Mathiassen Kim
Published: June 2022

This paper presents the design, control, and experimental evaluation of a novel fully automated robotic-assisted system for the positioning and insertion of a commercial full core biopsy instrument under guidance by ultrasound imaging. The robotic system consisted of a novel 4 Degree of freedom (DOF) add-on robot for the positioning and insertion of the biopsy instrument that is attached to a UR5-based teleoperation system with 6 DOF. The robotic system incorporates the advantages of both freehand and probe-guided biopsy techniques. The proposed robotic system can be used as a slave robot in a teleoperation configuration or as an autonomous or semi-autonomous robot in the future. While the UR5 manipulator was controlled using a teleoperation scheme with force controller, a reinforcement learning based controller using the Deep Deterministic Policy Gradient (DDPG) algorithm was developed for the add-on robotic system. The dexterous workspace analysis of the add-on robotic system demonstrated that the system has a suitable workspace within the US image. Two sets of comprehensive experiments including four experiments were performed to evaluate the robotic system’s performance in terms of the biopsy instrument positioning, and the insertion of the needle inside the ultrasound plane. The experimental results showed the ability of the robotic system for in-plane needle insertion. The overall mean error of all four experiments in the tracking of the needle angle was 0.446°, and the resolution of the needle insertion was 0.002 mm.
Comparative Analysis of Primary Prostate Cancer Treatment and Subsequent Metastatic Disease
Authors: Shahait Mohammed , Hamieh Nadine , Dobbs Ryan W. , Nguyen Tuan , Alshannaq Hamzeh , Kim Jessica , El-Fahmawi Ayah , Lee Daniel J. , Lee David I.
Published: June 2022

Objective

To compare the association between previous local treatment modalities and the progression to castrate-resistant prostate cancer (CRCP) and overall survival (OS) in men with newly diagnosed metastatic prostate cancer.

Methods

We conducted a retrospective analysis using a nationwide, de-identified electronic health record (EHR)-derived database (Flatiron). Eligible patients had previously received radiation therapy (RT) or radical prostatectomy (RP) for their local disease, and had progressed to metastatic disease. Stratified Kaplan-Meier estimates by local treatment were used to measure OS from the date of metastasis diagnosis. Cox proportional models were used to test the association between prior local treatment, progression to CRPC, and death, after adjusting for patient-and disease-specific parameters. Also, we conducted a propensity score-matched analysis.

Results

Of the 1,338 patients who met the inclusion criteria, 46% underwent RP with or without adjuvant RT and 54% received RT. Median follow up for RP group and RT group were 38.6 months (32.6-45.4) and 26.0 months (I24.3-29.9), respectively. After adjusting for patient-and disease-specific parameters, the patients who received RT had a higher risk of developing CRPC than those in the RP group 1.36 [1.05-1.76]. After propensity score matching and adjusting for patient and disease-specific parameters, men who received RT had higher risk of death compared to their counterparts (HR:1.36, 95% CI:1.1-1.65, P= 0.003)

Conclusion

Real-world data suggest that patients with metastatic disease who had undergone prior RP might have a lower risk of developing a castrate-resistant state and improved OS compared to patients who had received RT. Significant amount of bias limits validity and strength of our findings. Whether type of local treatment influence the disease behavior remains open question and should be answered only within randomized trial.
Secularism as a Project of Free and Equal Citizenship: Reflections on the Turkish Case
Authors: Gülalp Haldun
Published: June 2022

This article undertakes a defense of secularism, much maligned by postmodernists and multiculturalists. First, secularism as a normative political principle is conceptually distinguished from the discredited sociological theory of secularization and, second, it is treated as a project of free and equal citizenship. The conceptual discussion is complemented by an assessment of the Turkish case, falsely presented in the literature as a radical form of secularism. The article aims to show that a religious political movement, opposed to secularism, tends to be authoritarian and intolerant of diversity.
Accuracy vs. Practicality of Inertial Measurement Unit Sensors to Evaluate Motor Competence in Children
Authors: Lander Natalie , Nahavandi Darius , Toomey Nicole G. , Barnett Lisa M. , Mohamed Shady
Published: June 2022

The TGMD (i.e., Test of Gross Motor Development) has been considered as one of the gold standards of assessment tools for analysis of motor competence in children. However, it is rarely used by teachers in schools because the time, resources, and expertise required for one teacher to assess a class of students is prohibitive in most cases. A potential solution may be to automate the testing protocol using objective measures and inertial measurement unit sensors. An accurate method using 17 sensors to capture full body motion profiles and machine learning methods to objectively assess proficiency has been developed; however, feasibility of this method was low. Subsequently, a simplified method using four sensors (i.e., attached to wrists and ankles) was found to be effective, efficient, and potentially highly feasible for use in school settings. For some skills, however, not all skill criteria could be assessed. Additionally, misclassification on occasion, marred results. In the present paper we consider a previous experiment that used wireless motion capture to assess criteria from the TGMD-3. We discuss the advantages alongside the disadvantages of testing motor competence in children using sensors and consider the question—Can a compromise be struck between accuracy and feasibility?
Where Rabies Is Not a Disease. Bridging Healthworlds to Improve Mutual Understanding and Prevention of Rabies
Authors: Nadal Deborah , Hampson Katie , Lembo Tiziana , Rodrigues Rebecca , Vanak Abi Tamim , Cleaveland Sarah
Published: June 2022

Deeply embedded in local social, cultural, and religious settings, traditional healing is part of dog bite and rabies management in many rabies endemic countries. Faith healing, which usually encompasses a more holistic approach to health including physical, mental and social dimensions, is rare in the context of rabies. In Gujarat, Western India, the Hindu goddess Hadkai Mata is worshiped by low-caste communities as the Mother of Rabies in the event of a dog bite to a person or their livestock. This belief might influence people's attitudes and behaviors toward rabies prevention but has never been investigated. Through 31 in-depth interviews with healers and staff of Hadkai Mata temples, this paper explores the system of knowledge around dog and human rabies that is built and shared in these places of worship and healing. Qualitative and quantitative data were analyzed looking for convergences and divergences with the recently launched National Action Plan for dog-mediated Rabies Elimination. Results suggest that while the etiology of human rabies as a social illness is usually explained as the goddess's wish to correct misbehaving people and restore positive interpersonal relations, there is some appreciation for the biological processes of infection that lead to rabies as a physical disease. Hadkai Mata is believed to cure rabies if her patients undergo the necessary process of moral growth. Although conventional post-exposure prophylaxis is not opposed per se, it is often delayed by patients who seek traditional treatment first. Some reluctance was expressed toward mass dog vaccination because it is seen as an interference in how the goddess controls dogs, by enraging them—hence infecting them with rabies—and sending them to bite wrongdoers. Addressing these cultural perceptions is likely to be critical in achieving effective control of dog rabies in this region. The study highlights the value of multidisciplinary approaches in the control and elimination of rabies, as well as other zoonoses. This includes the importance of understanding different culturally- and religiously- mediated ways in which humans relate to animals; and looking for points of convergence and mutual understanding, upon which context-tailored, linguistically-accurate, locally acceptable, feasible and effective strategies can be designed.
11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells
Authors: Wang Lei , Sun Zhifeng , Xie Weina , Peng Chenglu , Ding Hongyan , Li Yu , Feng Shibin , Wang Xichun , Zhao Chang , Wu Jinjie
Published: June 2022

11S glycinin is a major soybean antigenic protein, which induces human and animal allergies. It has been reported to induce intestinal porcine epithelial (IPEC-J2) cell apoptosis, but the role of pyroptosis in 11S glycinin allergies remains unknown. In this study, IPEC-J2 cells were used as an in vitro physiological model to explore the mechanism of 11S glycinin-induced pyroptosis. The cells were incubated with 0, 1, 5, and 10 mg·ml−1 11S glycinin for 24 h. Our results revealed that 11S glycinin significantly inhibited cell proliferation, induced DNA damage, generated active oxygen, decreased mitochondrial membrane potential, and increased the NOD-like receptor protein 3 (NLRP-3) expression of IPEC-J2 cells in a dose-dependent manner. Further, IPEC-J2 cells were transfected with designed sh-NLRP-3 lentivirus to silence NLRP-3. The results showed that 11S glycinin up-regulated the silenced NLRP-3 gene and increased the expression levels of apoptosis-related spot-like protein (ASC), caspase-1, the cleaved gasdermin D, and interleukin-1β. The IPEC-J2 cells showed pyrolysis morphology. Moreover, we revealed that N-acetyl-L-cysteine can significantly inhibit the production of reactive oxygen species and reduce the expression levels of NLRP-3 and the cleaved gasdermin D. Taken together, 11S glycinin up-regulated NLRP-3-induced pyroptosis by triggering reactive oxygen species in IPEC-J2 cells.
Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China
Authors: Shi Kaichuang , Liu Huixin , Yin Yanwen , Si Hongbin , Long Feng , Feng Shuping
Published: June 2022

African swine fever virus (ASFV) causes contagious hemorrhagic disease of pigs with high morbidity and mortality. To identify the molecular characteristics of ASFV strains circulating in Guangxi province, southern China, a total of 336 tissue samples collected from 336 domestic pigs that died as a result of severe hemorrhagic disease during 2019–2020 were tested for ASFV. Furthermore, 66 ASFV strains were genetically characterized by sequence analysis of the C-terminal region of B646L (p72) gene, the complete E183L (p54) gene, the variable region of EP402R (CD2v) gene, the central variable region (CVR) of B602L gene, the full MGF505-2R gene, and the tandem repeat sequence (TRS) within intergenic region (IGR) between the I73R and I329L (I73R/I329L) genes. Phylogenetic analysis revealed that the ASFV strains from Guangxi province belonged to genotypes I and II based on the B646L (p72) and E183L (p54) genes, and there were eight different tetrameric TRS variants based on the CVR of B602L gene. Phylogenetic analysis of the EP402R (CD2v) gene revealed that these ASFV strains belonged to serogroups 4 and 8. Eight of the 66 strains belonged to genotype I and serogroup 4, and showed deletion of whole MGF505-2R gene. The sequence analysis of the IGR between the I73R/I329L genes showed that IGR II and III variants were co-circulating in Guangxi province. The results indicated that ASFV strains circulating in Guangxi province during 2019–2020 outbreaks showed high genetic diversity, of which genotypes I and II, as well as serogroups 4 and 8, were simultaneously circulating in Guangxi province, and there existed wild-type and naturally gene-deleted strains in the field. This is the first detailed report on the molecular characterization of the ASFV strains circulating in southern China, and serogroup 4 in China.
Soundscape Approach in the Seaport of Ancona: A Case Study
Authors: Di Loreto Samantha , Serpilli Fabio , Lori Valter
Published: June 2022

Today, the art of knowing how to listen is more urgent than ever. The perceptive sound system of the human being is stimulated daily by countless artificial sounds that dominate natural ones. When it comes to the idea of the soundscape, the terminology was initially referred to by composer and environmentalist Raymond Murray Shafer, who defined “soundscape” as a relationship between the ear, humans, built environments, and society. This paper aims to apply the sound landscape approach in the seaport area of Ancona (Italy); a large area, frequented daily by many people, which is divided into passenger and ferry terminals, container terminals, plants for solid bulk, and commercial and recreational activities. The purpose of the study was to evaluate the perception that a human has of the urban layout of the port area by correlating the parameters of traditional acoustics with psychoacoustics. To evaluate the subjective parameters, a questionnaire was developed and applied, enabling the analysis of demographic and behavioral factors such as age, visit frequency, and length of stay of the participants in the clusters of the seaport. This way, it was possible to give an indication of the sound quality of the different clusters of the port area, from an acoustic and emotional point of view, and this information could be particularly helpful in the planning phase of new attractions for the Ancona seaport.
Performance-Optimized Dielectric Elastomer Actuator System with Scalable Scissor Linkage Transmission
Authors: Bruch Daniel , Willian Tobias Pascal , Schäfer Hendrik Cornelius , Motzki Paul
Published: June 2022

Thanks to their outstanding properties, in the last few years Dielectric Elastomer Actuators (DEAs) have increasingly attracted the interest of the scientific community and generated a surge in the effort devoted to their industrialization. Compared to conventional actuator systems, DEAs are based on inexpensive and widely available polymeric materials, which make them potentially attractive from a market perspective. However, DEA systems with a given layout and dimensions have a fixed force-stroke response that is only suitable for a specific load profile. This leads to a wide variety of designs combined with small production volumes and high costs, limiting the competitive advantage. This work addresses this issue by proposing a combination of DEA systems with compliant scissor linkage transmission mechanisms, which provide linear stroke and force scaling and simultaneously maintain performance optimization by leaving the convertible energy density of the DEA unaffected. For this purpose, three systems are designed, based on a same strip-shaped DEA combined with inclined buckled beam biasing mechanisms. Two of the systems are coupled with scissor linkages that offer transmission ratios of 3:1 and 1:3, respectively, to adapt the system to different load profiles. The system design is explained in detail, and the functional principle is validated through experiments.
Spatial Evolution, Driving Mechanism, and Patch Prediction of Grain-Producing Cultivated Land in China
Authors: Zhu Zhiyuan , Duan Jiajia , Li Ruolan , Feng Yongzhong
Published: June 2022

China has implemented strict policies for protecting cultivated land, and the Chinese government has focused on the non-grain production (NGP) of cultivated land. This study aimed to analyze the spatial evolution law of grain-producing cultivated land (GPCL) in China between 2000 and 2018, explore the mechanism of GPCL, and simulate the spatial characteristics of GPCL in 2036. We used the Geographic Information System (GIS) and a patch-generating land-use simulation model, a new model that proposes a land expansion analysis strategy by improving previous rule-mining methods. China’s grain production rate (GPrate) shows a gradual upward trend between 2000 (36.98%) and 2018 (47.18%). The mutual conversion of GPCL and non-grain-producing cultivated land (NGPCL) are the primary transfer types. The evolution of GPCL is driven by climatic, economic, and social factors, of which population density is the most important factor. GPCL expansion patches are distributed in densely populated, economically developed, and warm and humid plain areas. Further, the simulation results showed that the GPrate in 2036 is estimated to be 41.39%, with GPCL transfer-in significantly exceeding the amount transferred out. Our results further cultivated land evolution-associated research and provide a basis for formulating scientific land-use policies for cultivated land protection for other countries.
Design and Experiment of Full-Tray Grafting Device for Grafted Melon Seedling Production
Authors: Fu Xiaohu , Shi Jiahao , Huang Yuan , Zhu Enzhao , Bie Zhilong , Lin Weiguo
Published: June 2022

At present, the existing vegetable grafting machines are cutting and grafting operations for a single plant or row. They need to manually or automatically grab the seedlings, and their grafting efficiency is not significantly higher than that of manual grafting techniques. In this paper, grafted melon seedlings were the subject of the research. Based on the splice-grafting method, we designed a clamping and positioning device for full-tray seedlings and a mechanical device to realize full-tray grafting by locating the vegetable seedlings and completing the full-tray grafting process without damaging the seedlings. The results show that the cutting success rate for pumpkin rootstock and for melon scion reached 100% and 92%, respectively. The lengths of the long axis of the section of the rootstock and the scion were 6.2–7.7 mm, and the cutting angle was maintained at 30 degrees, thereby able to meet the requirements of the grafting method. The average grafting efficiency for the rootstock and scion were 2134 plants/h, and the average grafting success rate was 67%.
Estimation of Cotton Nutrient Uptake Based on the QUEFTS Model in Xinjiang
Authors: Yibati Halihashi , Zhang Yan , Li Qingjun , Xu Xingpeng , He Ping
Published: June 2022

The Xinjiang cotton-producing region is the main cotton-growing region in China; however, the combination of excessive application but low utilization of fertilizers has restricted its agricultural development. Estimating the balanced nutrient requirements for cotton is essential to increase its yield and nutrient use efficiency. The results from 372 field experiments performed between 1996 and 2019 were used to build a QUEFTS (quantitative evaluation of the fertility of tropical soils) model to investigate relationships between cotton seed yield and nutrient accumulation, with the data from 2017 to 2020 used to validate the model. The results demonstrated that the QUEFTS model predicted a linear relationship between target yield and nutrient uptake until the target yield reached about 60–70% of the potential yield. To produce 1000 kg of seed cotton, 28.3 kg N, 6.1 kg P, and 29.6 kg K were required for cotton, and the corresponding ratio of N, P, and K was 4.64:1:4.85. The corresponding internal efficiencies (IEs) for N, P, and K were 35.4, 163.2, and 33.7 kg/kg, respectively. Field validation indicated that the QUEFTS model could be used to estimate nutrient uptake at a targeted yield and contribute to developing a fertilizer recommendation strategy for Xinjiang cotton production.
Monitoring of Iron Ore Quality through Ultra-Spectral Data and Machine Learning Methods
Authors: Silva Ana Cristina Pinto , Coimbra Keyla Thayrinne Zoppi , Filho Levi Wellington Rezende , Pessin Gustavo , Correa-Pabón Rosa Elvira
Published: June 2022

Currently, most mining companies conduct chemical analyses by X-ray fluorescence performed in the laboratory to evaluate the quality of Fe ore, where the focus is mainly on the Fe content and the presence of impurities. However, this type of analysis requires the investment of time and money, and the results are often available only after the ore has already been sent by the processing plant. Reflectance spectroscopy is an alternative method that can significantly contribute to this type of application as it consists of a nondestructive analysis technique that does not require sample preparation, in addition to making the analyses available in more active ways. Among the challenges of working with reflectance spectroscopy is the large volume of data produced. However, one way to optimize this type of approach is to use machine learning techniques. Thus, the main objective of this study was the calibration and evaluation of models to analyze the quality of Fe from Sinter Feed collected from deposits in the Carajás Mineral Province, Brazil. To achieve this goal, machine learning models were tested using spectral libraries and X-ray fluorescence data from Sinter Feed samples. The most efficient models for estimating Fe were the Adaboost and support vector machine and our results highlight the possibility of application in the samples without the need for preparation and optimization of the analysis time, providing results in a timely manner to contribute to decision-making in the production chain.
Assessment of Neurodegenerative Changes in Turkeys Fed Diets with Different Proportions of Arginine and Methionine Relative to Lysine
Authors: Krauze Magdalena , Ognik Katarzyna , Mikulski Dariusz , Jankowski Jan
Published: June 2022

We postulated that the use of optimal levels and proportions of Arg and Met relative to a low or high concentration of Lys in diets for meat turkeys would reduce the occurrence of metabolic disturbances in the nervous tissue that can lead to neurodegenerative changes. The aim of the study was to determine the effect of various proportions of Lys, Arg, and Met in diets for turkeys, with a low content of Lys in accordance with NRC (Experiment 1) recommendations, and in diets with high Lys levels that are close to the recommendations of breeding companies (Experiment 2) on selected indicators of potential neurodegenerative effects in the brain and liver of turkeys. The Experiment 1 and Experiment 2 was conducted using 864 day-old turkey chicks randomly assigned to six groups, in eight replicates (6 groups × 18 birds × 8 replicates). A full description of the methodology can be found in previously published papers using the same experimental design. Indicators informing about the presence or advancement of neurodegenerative processes in the nervous tissue were determined in the brain and liver (level of: AChE, amyloid-β, GAChE, Tau protein, LRP1, and the degree of DNA methylation). It was established that in the case of both a low (National Research Council, NRC) and a high (British United Turkeys, BUT) level of Lys in the diet of turkeys, the Arg level can be reduced to 90% of the Lys level and Met to 30% of the Lys level, because this does not cause neurodegenerative changes in turkeys. Unfavorable neurodegenerative changes may appear if the Arg level is increased from 100 to 110% of the Lys level recommended by the NRC. However, due to the lack of such a relationship when Arg is increased from 100 to 110% of the Lys level recommended by BUT, at this stage of research no definitive conclusions can be drawn regarding the risk of neurodegenerative changes caused by increasing Arg in the diet of turkeys.
Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by 1H-NMR Spectroscopy
Authors: Zhu Chenglin , Jin Lei , Luo Bo , Zhou Qiang , Dong Li , Li Xiaoyan , Zhang Hemin , Huang Yan , Li Caiwu , Zou Likou , Laghi Luca
Published: June 2022

As an assisted breeding technique, artificial insemination has become the main effective practical approach in the captive breeding programs of giant panda worldwide. The composition of seminal plasma plays an important role in the success of breeding. The present work is the first attempt to characterize, by proton magnetic resonance spectroscopy (1H-NMR), the metabolome of healthy giant panda seminal plasma. A total of 35 molecules were quantified, with the concentration of 2,3-butanediol being significantly different between individuals younger than 8 years and older than 13 years, and other distinct age-related trends were highlighted by a multivariate analysis. Isopropanol’s concentration was significantly linked to estrus stages. Besides, the variations in the metabolome’s profile during storage were also evaluated. This study may serve as a reference for further research wishing to shed light on the biological mechanisms affecting giant panda sperm’s overall quality and may ultimately lead to novel approaches to giant panda artificial insemination.
Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
Authors: Zhang Qingxun , Zhong Zhenyu , Xia Zhiqiang , Meng Qinghui , Shan Yunfang , Guo Qingyun , Cheng Zhibin , Zhang Peiyang , He Hongxuan , Bai Jiade
Published: June 2022

Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause zoonotic diseases in humans and animals. Despite receiving increasing attention in relation to domestic animals, there has been limited information on the infection burden of E. bieneusi in cervids. Altogether, 215 fecal samples collected from four deer species in Beijing, China were examined by nested- Polymerase Chain Reaction (PCR)targeting the internal transcribed spacer (ITS) region. The overall prevalence of E. bieneusi in deer was 21.9% (47/215), with 30.0% (24/80) in Pere David’s deer, 27.3% (15/55) in fallow deer, 12.5% (5/40) in sika deer, and 7.5% (3/40) in Chinese water deer. Thirteen E. bieneusi genotypes were identified, including six known (HLJD-V, MWC_d1, BEB6, CGC2, JLD-XV, and HND-I) and seven novel genotypes (BJED-I to BJED-V, BJFD, and BJCWD). A phylogenetic analysis showed that 38.3% of the isolates belonged to zoonotic Group 1. In addition, E. bieneusi infection was first detected in fallow deer and Chinese water deer, which could act as potential zoonotic reservoirs. Our findings suggest that E. bieneusi circulates in deer and might be of importance to public health.
The Role of Peroxisome Proliferator-Activated Receptors in PGF2α-Induced Luteolysis in the Bovine Corpus Luteum
Authors: Socha Barbara Maria , Łada Piotr , Jończyk Agnieszka Walentyna , Korzekwa Anna Justyna , Skarżyński Dariusz Jan
Published: June 2022

The participation of peroxisome proliferator-activated receptors (PPARs) in ovarian function in cattle is still not fully understood. The aim of this in vitro study was to determine: (i) the immunolocalization, mRNA expression and tissue concentration of PPARα, PPARδ and PPARγ in the bovine corpus luteum (CL) (n = 40) throughout the estrous cycle, and (ii) the involvement of PPAR in PGF2α-induced processes related to luteolysis. CL (n = 9) explants were cultured in the presence of PPAR antagonists (10−5 M) in combination with or without PGF2α receptor antagonist (10−5 M) and PGF2α (10−6 M). The mRNA and protein expression of PPARs was evaluated through qPCR, IHC, and ELISA, respectively. The results showed that PPAR mRNA and protein expression differed according to the luteal stages. PGF2α upregulated PPARδ and PPARγ mRNA expression in the bovine CL in vitro, whereas PPARγ increased the inhibitory effect of PGF2α by decreasing progesterone secretion and the mRNA expression of hydroxy-delta-5-steroid dehydrogenase, 3 β- and steroid delta-isomerase 1 (HSD3B1) in the CL explants; mRNA transcription of tumor necrosis factor α (TNFα) and inducible nitric oxide synthase (iNOS) was increased. The obtained results indicate that the mRNA and protein expression of PPARs changes in the bovine CL throughout the estrous cycle and under the influence of PGF2α. We suggest that isoform γ, among all examined PPARs, could be a factor involved in the regulation of PGF2α-induced processes related to luteolysis in the bovine CL. Further studies are needed to understand the role of PPAR in luteal regression in the CL of cattle.