bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacto da gestão do consumo de água no Hospital Estadual Américo Brasiliense: um estudo piloto
Authors: Neri Júnior Wilson Antônio , Varallo Fabiana , Nadai Tales
Published: May 2020

Objetivos: Avaliar a redução da extração de água no poço artesiano após a instalação de medidores de vazão em pontos de distribuição hídrica num hospital. Métodos: Conduziu-se estudo piloto de intervenção do tipo antes e depois no Hospital Estadual Américo Brasiliense, localizado no interior do estado de São Paulo. A gestão da água iniciou-se em 2010 e realizou-se por meio do monitoramento de sete hidrômetros pré-equipados para sistema de telemetria, com relojoaria mecânica e sistema de turbinas, dos quais quatro foram instalados em 2016. Em março de 2017, foram instalados chuveiros e torneiras para limpeza com redutores de vazão em todo o hospital e arejadores em todas as torneiras para higienização das mãos. Analisou-se o impacto dos acessórios comparando-se a captação de água (m3) do poço artesiano. Estimou-se o retorno financeiro após a implantação das medidas. Resultados: Observou-se redução entre 21% e 42% (600 e 1.444 m³) do total do consumo hídrico e em 8.000 m3 de água por ano na extração do aquífero. Com a economia entre 800 e 1.000 m3 /mês de água, obteve-se retorno dos investimentos em 13 dias da implantação dos acessórios, pois o montante investido para compra e instalação dos equipamentos foi de 10.955 mil reais e a economia advinda da redução do consumo hídrico foi de 24.302 mil reais. Conclusão: A instalação de medidores de vazão é custo-efetiva para economia de água e financeira em hospitais. As melhorias contribuem para aumentar a credibilidade do desenvolvimento de projetos sustentáveis e a viabilidade de investimentos em ações ambientais.
Educación como disciplina y como objeto de estudio: aportes para un debate
Authors: Mora-Olate María Loreto
Published: May 2020

El ensayo tiene por objetivo dar cuenta de un debate que parece persistir acerca del rango científico de la educación o de si es más correcto hablar de «ciencia» o «ciencias de la educación». Mediante una revisión bibliográfica se intenta responder a la pregunta: ¿en qué se reconoce la educación? Se concluye que, debido a la complejidad del fenómeno educativo, hablar de «ciencias de la educación» no constituiría subsidiariedad, sino más bien una sensata y necesaria interdependencia con las demás disciplinas, que permiten estudiar la educación en su real dimensión social, histórica, biológica y psicológica.
How methodological frameworks are being developed: Evidence from a scoping review
Authors: McMeekin Nicola , Wu Olivia , Germeni Evi , Briggs A
Published: May 2020

Background: A methodological framework is a structured guide to completing a process or procedure. Although the benefits of using methodological frameworks are increasingly recognised, to date, there is no formal definition of what constitutes a ‘methodological framework’, nor is there any published guidance on how to develop one. This study’s aims are to: (a) map the existing landscape on the use of methodological frameworks; (b) identify approaches used for the development of methodological frameworks and terminology used; and (c) provide suggestions for developing future methodological frameworks. We took a broad view and did not limit our study to methodological frameworks in research and academia.Methods: A scoping review was conducted, drawing on Arksey and O’Malley’s methods and more recent guidance. We systematically searched two major electronic databases (MEDLINE and Web of Science), as well as grey literature sources and the reference lists and citations of all relevant papers. Study characteristics and approaches used for development of methodological frameworks were extracted from included studies. Descriptive analysis was conducted.Results: We included a total of 30 studies, representing a wide range of subject areas. The most commonly reported approach for developing a methodological framework was ‘Based on existing methods and guidelines’ (66.7%), followed by ‘Refined and validated’ (33.3%), ‘Experience and expertise’ (30.0%), ‘Literature review’ (26.7%), ‘Data synthesis and amalgamation’ (23.3%), ‘Data extraction’ (10.0%), ‘Iteratively developed’ (6.7%) and ‘Lab work results’ (3.3%). There was no consistent use of terminology; the studies included a range of terms for ‘methodological framework’, which were also used interchangeably within studies.Conclusions: Although no formal guidance exists on how to develop a methodological framework, this scoping review found an overall consensus in approaches used, which can be broadly divided into three phases: (a) identifying data to inform the methodological framework; (b) developing the methodological framework; and (c) validating, testing and refining the methodological framework. Based on these phases, we provide suggestions to facilitate the development of future methodological frameworks.Trial registration: Not applicable
Evaluationof Integrated Modular Teaching in Chinese Ophthalmology Trainee Courses
Authors: Xin Wei , Zou Yuxian , Ao Yong , Cai Yu , Huang Zheqian , Li Miaoling , Xu Chaochao , Jia Yu , Yang Ying , Yang Yangfan , Lin Haotian
Published: May 2020

Background: Before attending ophthalmology trainee courses in Zhongshan Ophthalmic Centre, the medical students from Sun Yat-sen University had finished two years of premedical education after the six-year medical courses including basic medical courses, clinical medical courses, clerkship, and research training in medical college. Integrated modular teaching using different problem-based teaching methods in ophthalmology was designed by the teaching steering committee of Zhongshan Ophthalmic Centre. This study aimed to evaluate the effectiveness and satisfaction scales of the integrated modular teaching among the trainee students. Methods: A total of 100 medical students attending ophthalmology trainee courses in Zhongshan Ophthalmic Centre were enrolled and randomly allocated into 4 groups according to the teaching arrangement. The trainee courses consisted of several sessions delivered in multiple methods, such as "flipped classroom" session and team-based learning session. The pre- and post-class tests were delivered to evaluate the effectiveness of the integrated modular teaching. The satisfaction survey questionnaire was collected from all participants to investigate the degree of satisfaction. Results: Compared with the first-day-test score, the total last-day-test score was significantly improved by a paired t-test (t=3.288, P=0.001). Nineteen students obtained a significant improvement in ranking increased by more than 10 in the last-day-test, whereas they failed to obtain a higher average score for daily performance than other students (t=0.469, P=0.654). According to the participant satisfaction questionnaires, these innovative teaching methods were considered as effective and satisfactory. Conclusions: Integrated modular teaching in ophthalmology trainee courses is effective and widely recognized by the medical college students.
Institutional Delivery Service Utilization and Associated Factors in Ethiopia: A Systematic Review and Meta Analysis
Authors: Nigusie Adane , Azale Telake , Yitayal Mezgebu
Published: May 2020

Background: Different primary studies in Ethiopia showed the weight of institutional delivery service utilization and the factors associated with it. However, variation among those studies was seen. This study was aimed to estimate the pooled national prevalence and associated factors of Institutional delivery service utilization in Ethiopia. Methods: PubMed, Cochrane library, Web of Science, and Google Scholar were searched. A funnel plot and Egger’s regression test were used to see publication bias. I-squared statistic was applied to check heterogeneity of studies. A weighted Dersimonian laired random effect model was applied to estimate the pooled national prevalence and the effect size of associated factors. Result: A total of twenty four studies were included in this review. The pooled prevalence of Institutional delivery service utilization was 31% (95% Confidence interval (CI): 30%, 31.2%; I 2 = 0.00%). Attitude towards institutional delivery of 3 studies(Adjusted Odd Ratio (AOR)=2.83; 95% CI 1.35,5.92 ), maternal age at first pregnancy of 4 studies(AOR= 3.59; 95% CI 2.27,5.69 ), residence setting of 7 studies (AOR= 3.84; 95% CI 1.31, 11.25), educational status of 5 studies(AOR=2.91;95% 1.88,4.52), availability of information source of 6 studies(AOR= 1.80;95% CI 1.16,2.78), ANC follow-up of 13 studies((AOR= 2.57 95% CI 1.46,4.54 )), frequency of ANC follow up of 4 studies(AOR= 4.04;95% CI 1.21,13.46), knowledge of institutional delivery & danger signs during pregnancy of 11 studies((AOR=3.04;95% CI 1.76 ,5.24 )) and place of birth the most recent birth of 4 studies(AOR=8.44;95% CI 5.75,12.39) were identified factors of institutional delivery service utilization. Conclusions: The findings of this review found several modifiable factors such as empowering women through education, promoting antenatal care to prevent home delivery, increasing awareness of women through mass media and making services more accessible would likely to increase utilization of institutional delivery.
Association of Dietary Inflammatory Index with Cardiovascular Disease in Kurdish Adults: Results of a Prospective Study on Ravansar Non-Communicable Diseases
Authors: Ayenehpour Azad , Nazar Mehdi Moradi , Samadi Mehnoosh , Hamzeh Behrooz , Najafi Farid , Karimi Sheno , Faraji Fakhereh , Darbandi Mitra , Pasdar Yahya
Published: May 2020

Background: Various diets and dietary compounds, through their inflammatory properties, are involved in the pathogenesis of chronic diseases including Cardiovascular Diseases (CVDs). Dietary Inflammatory Index (DII) can evaluate the inflammatory properties of diet. The purpose of this study was to determine the association between DII and CVDs in participants of the Ravansar Non-Communicable Diseases (RaNCD)cohort study, Kermanshah, Iran. Materials: The present cross-sectional study was conducted using the recruitment phase data of the RaNCD cohort study on 6369 participants aged 35 to 65 years. The Food Frequency Questionnaire (FFQ) was used to assess diet. The DII scores were calculated using FFQ data. Participants with a history of myocardial infarction, stroke and coronary artery disease, and/or taking medications for the CVDs were considered as the CVDs patients. Results: Of the 6369 studied participants, 9% (n=579) had CVDs history. The mean DII score in this study was -0.84±1.6. Odds ratio (OR) of CVDs in female was 1.6 times higher than in male (CI 95%=1.3-1.9), which this association was remained after adjusting for confounding variables (OR=1.5, CI%=1.2-1.9). The risk of CVDs in the fourth quartile of DII was 1.4 times higher than the first quartile of DII (OR: 1.4, CI 95%=1.1-1.8). We found that higher adhere to DII was associated with risk of CVDs. Conclusion: Given the role of diet through inflammatory properties on the risk of CVDs, it is highly recommended to use DII as an appropriate index to measure the effect of diet on CVDs. In addition, a diet with lower DII is healthier diet for cardiovascular health.
The adverse maternal and perinatal outcomes of adolescent pregnancy: a cross sectional study in Hebei, China
Authors: Zhang Ting , Wang Huien , Wang Xinling , Yang Yue , Zhang Yingkui , Tang Zengjun , Wang Li
Published: May 2020

Background: The adverse pregnancy outcomes caused by teenage pregnancy are major public health problems with significant social impact. While China is the most populous country in the world, and 8.5% of the women aged 10-50 years are adolescent women, we aimed to analyze the adverse maternal and perinatal outcomes of the adolescent pregnancy in Hebei Province, China.Methods: There were 238,598 singleton pregnant women aged 10-34 years from January 1, 2013 to December 31, 2017 in the database of Hebei Province Maternal Near Miss Surveillance System (HBMNMSS). The 238,598 pregnant women were divided into two groups: adolescent group (aged 10-19 years) and adult group (aged 20-34 years). The adolescent group was divided into two subgroups (aged 10-17 years, aged 18-19 years), the adult group was divided into two subgroups (aged 20-24 years, aged 25-34 years). We compared the risk of adverse pregnancy outcomes using univariate and multivariate logistic regression. We also made a stratified analysis of nulliparous and multiparous adolescent pregnancy. Results: Compared with women aged 20-34 years, women aged 10-19 years had lower risk of cesarean delivery [adjusted risk ratio (aRR): 0.75, 95% confidence interval (CI): 0.70-0.80], gestational diabetes mellitus (GDM) (aRR: 0.55, 95%CI: 0.41-0.73). Women aged 10-19 years had higher risk of preterm delivery (aRR: 1.76, 95%CI: 1.54-2.01), small for gestational age (SGA) (aRR: 1.19, 95%CI: 1.08-1.30), stillbirth (aRR: 2.58, 95%CI: 1.83-3.62), neonatal death (aRR: 2.63, 95%CI: 1.60-4.32). The adolescent women aged 10-17 years had significantly higher risk of stillbirth (aRR: 5.69, 95%CI: 3.36-9.65) and neonatal death (aRR: 7.57, 95%CI: 3.74-15.33) compared with the women aged 25-34 years. Younger adults (20-24 years) also had higher risks of preterm delivery (aRR: 1.26, 95%CI: 1.20-1.32), stillbirth (aRR: 1.45, 95%CI: 1.23-1.72), and neonatal death (aRR: 1.51, 95%CI: 1.21-1.90) compared with women aged 25-34 years. The structural equation model showed that preterm delivery and cesarean delivery had an indirect effect on neonatal death in adolescent pregnancy.Conclusions: The adolescent pregnancy was related to adverse perinatal (fetal and neonatal) outcomes, such as preterm delivery, stillbirth and neonatal death, especially in younger adolescent pregnancies.
Depression and relevant influencing factors among elderly adults with different sleep quality in pension institutions in Northeastern China
Authors: Zhang Jun , Zhang Yingying , Luan Zhenggang , Zhang Xiujie , Jiang Haoran , Wang Aiping
Published: May 2020

Background: China has the largest aged population in the world, and the elderly adults who live in pension institutions are more likely to suffer from mental disorders than other elderly adults. The current study aimed to explore the distribution of the depression among the aged population with different sleep conditions in pension institutions in Northeastern China.Methods: We conducted a cross-sectional study in Northeastern China from May to September 2017 using a multistage sampling method and 507 elderly people without cognitive impairment in six pension institutions were interviewed. The Pittsburgh Sleep Quality Index (PSQI) and Geriatric Depression Scale (GDS) were adopted to collected the information of sleep quality and depression. We used logistic regression to analyze the factors influencing depression among the elderly adults with poor or good sleep quality.Results: The overall depression rate among elderly adults was 21.7%. The logistic regression analysis revealed that marital status, chronic disease, regular exercise, physical pain, filial piety and chewing ability had significant effects on the depression of the elderly with good sleep quality. Loneliness, self-care ability, chewing ability and chronic diseases had significant effects on depression of the elderly with poor sleep quality. Conclusion: The prevalence of depressive symptoms in the elderly is not high. However, different factors influenced depression among elderly adults depending on their sleep quality. Therefore, the risk factors of depression among elderly adults should be analyzed separately.
Low cyclosporine concentrations in children and time to acute graft versus host disease
Authors: Chung Eun Kyung , Yee Jeong , Kim Jae Youn , Gwak Hye sun
Published: May 2020

Background: Achievement of target blood concentrations of cyclosporine (CsA) early after transplantation is known to be highly effective for reducing the incidence of acute graft versus host disease (aGVHD). However, no research has been conducted for predicting aGVHD occurrence with low CsA concentrations at different time periods. The objective of this study was to investigate the risk of aGVHD according to low CsA concentrations at lag days in children with allogenic hematopoietic stem cell transplantation (HSCT). Methods: The records of 61 consecutive children who underwent allogeneic HSCT and received CsA as prophylaxis against aGVHD between May 2012 and March 2015 were retrospectively evaluated. The main outcome was any association between low CsA concentrations at lag days and aGVHD occurrence, which was examined for the first month after transplantation. Mean CsA concentrations at three lag periods were calculated: lag days 0-6, 7-13, and 14-20 before aGVHD occurrence.Results: Patients whose mean CsA concentrations at lag days 0-6 did not reach the initial target concentration had 11.0-fold (95% confidence interval [CI]: 2.3-51.9) greater incidence of aGVHD. In addition, the AORs of low CsA concentrations at lag days 7-13 and 14-20 for developing aGVHD were 108.2 (95% CI: 7.7-1515.5) and 12.1 (95% CI: 1.1-138.1), respectively. Conclusions: After low CsA concentrations are detected, careful attention needs to be paid to prevent aGVHD.
Overexpression of an aquaporin gene PvPIP2;9 increased biomass yield and protein content, improved drought tolerance and water use efficiency and affected other PIP2 genes’ expression in switchgrass (Panicum virgatum L.)
Authors: Zhang Jing , Wen Wuwu , Li Hui , Lu Qiyu , Xu Bin , Huang Bingru
Published: May 2020

Background: Switchgrass ( Panicum virgatum L.) is a prime candidate for non-grain-based bioenergy feedstock production. Improved drought tolerance and higher water use efficiency are important for its successful field establishment and production, especially on marginal lands. Aquaporins are key channels and regulators for water transportation and maintenance of cellular water status. In this study, the functional role of an aquaporin gene, PvPIP2;9 , in switchgrass was studied. Results: Expression of PvPIP2;9 was regulated by diurnal oscillation and osmotic stress. Constitutive over-expressing PvPIP2;9 in switchgrass significantly improved its leaf length, plant height, above-ground biomass, biomass protein contents, and cellulose contents in stressed plants. Under 21 days of drought treatment, transgenic plants showed less electrolyte leakage rates, but higher relative water contents, photochemical efficiencies, and chlorophyll contents, indicating that PvPIP2;9 positively regulated plant drought tolerance and water use efficiency. Moreover, expression patterns of all 14 switchgrass PIP2 subfamily genes were checked during the drought treatment, and the result showed that over-expressing PvPIP2;9 also affected transcript levels of most other PIP2 genes. Conclusions: Together, this study showed that improved biomass yield, drought tolerance and higher water use efficiency can be achieved by manipulating the expression level of PvPIP2;9 and also suggested PIP2 subfamily genes were transcriptionally regulated in a coordinated manner.
Potentialities of multi-b-values diffusion-weighted imaging for predicting efficacy of concurrent chemoradiotherapy in cervical cancer patients
Authors: Liu Bing , Ma Wan-Ling , Zhang Guang-Wen , Sun Zhen , Wei Meng-Qi , Hou Wei-Huan , Hou Bing-Xin , Wei Li-Chun , Huan Yi
Published: May 2020

Purpose: To testify whether multi-b-values DWI can be used to ultra-early predict treatment response of CCRT in cervical cancer patients and to assess the predictive ability of concerning parameters. Methods: 53 patients with biopsy proved cervical cancer were retrospectively recruited in this study. All patients underwent pelvic multi-b-values DWI before and at the 3 rd day during treatment. Tumor diffusion parameters were calculated by mono-exponential (apparent diffusion coefficient, ADC), bi-exponential (D slow , D fast and f p ) and stretched exponential (distributed diffusion coefficient, DDC and α) models. Treatment response was assessed based on RECIST v1.1 at 1 month after the completion of whole CCRT. Parameters were compared using independent t test or Mann-Whitney U test as appropriate. Receiver operating characteristic (ROC) curves was used for statistical evaluations. Results: ADC-T0 (p=0.02), D slow -T0 (p<0.01), DDC-T0 (p=0.03), ADC-T1 (p<0.01), D slow -T1 (p<0.01), ΔADC (p=0.04) and Δα (p<0.01) were significant lower in non-CR group patients. ROC analyses showed that ADC-T1 and Δα exhibited high prediction value, with area under the curves of 0.880 and 0.869, respectively. Conclusion: Multi-b-values DWI can be used as a noninvasive technique to assess and predict treatment response in cervical cancer patients at the 3 rd day of CCRT. ADC-T1 and Δα can be used to differentiate good responders from poor responders.
PSD-93 mediates the crosstalk between neuron and microglia and facilitates acute ischemic stroke injury by binding to CX3CL1
Authors: zhang qingxiu , He Lei , Chen Mo , Yang Hui , Cao Xiaowei , Liu Xiaomei , Hao Qi , Chen Zhengwei , Liu Tengfei , Wei Xiu-e , Rong Liangqun
Published: May 2020

Background:Postsynaptic density 93 (PSD-93) mediates glutamate excitotoxicity induced by ischemic brain injury, which then induces microglial inflammatory response. However, the underlying mechanisms of how PSD-93 mediates the crosstalk between neurons and microglia in the postsynaptic dense region remain elusive. CX3 chemokine ligand 1 (CX3CL1) is a chemokine specifically expressed in neurons while its receptor CX3CR1 is highly expressed in microglia. In this study, we aimed to investigate the role of PSD-93 and CX3CL1 interaction in the crosstalk between neuron and microglia in acute ischemic stroke.Methods:Male C57BL/6 mice were used to establish middle cerebral artery occlusion model and co-immunoprecipitation and immunoblotting were used to detect the binding of PSD-93 and CX3CL1 at different time points following cerebral ischemic/reperfusion (I/R). ELISA was used to detect soluble CX3CL1. Yeast two-hybrid and co-immunoprecipitation were used to identify special amino acid sequences responsible for the interaction between PSD-93 and CX3CL1. Finally, a fusion small peptide Tat-CX3CL1 was designed to inhibit PSD-93 and CX3CL1 interaction.Results:The binding of PSD-93 and CX3CL1 peaked at 6 h after I/R. The binding sites were located in the 420-535 amino acid sequence of PSD-93 and 357-395 amino acid sequence of CX3CL1. Tat-CX3CL1 (357-395aa) could inhibit the interaction of PSD-93 and CX3CL1 and inhibited the pro-inflammatory cytokine IL-1β and TNF-α expression and provided neuroprotection following reperfusion.Conclusions:PSD-93 binds CX3CL1 to activate microglia and initiate neuroinflammation. Specific blockade of PSD-93-CX3CL1 interaction reduces I/R induced neuronal cell death, and provides a new therapeutic target for ischemic stroke.
Can we walk away from cardiovascular disease risk or do we have to ‘huff and puff’? A cross-sectional compositional accelerometer data analysis among adults and older adults in the Copenhagen City Heart Study
Authors: Johansson Melker Staffan , Søgaard Karen , Prescott Eva , Marott Jacob Louis , Schnohr Peter , Holtermann Andreas , Korshøj Mette
Published: May 2020

Background: It is unclear whether walking can decrease cardiovascular disease (CVD) risk or if high intensity physical activity (HIPA) is needed, and whether the association is modified by age. We investigated how sedentary behaviour, walking, and HIPA, were associated with systolic blood pressure (SBP), waist circumference (WC), and low-density lipoprotein cholesterol (LDL-C) among adults and older adults in a general population sample using compositional data analysis. Specifically, the measure of association was quantified by reallocating time between sedentary behaviour and 1) walking, and 2) HIPA.Methods: Cross-sectional data from the fifth examination of the Copenhagen City Heart Study was used. Using the software Acti4, we estimated daily time spent in physical behaviours from accelerometer data worn 24 h/day for 7 days (i.e., right frontal thigh and iliac crest; median wear time: 6 days, 23.8 h/day). SBP, WC, and LDL-C were measured during a physical examination. Inclusion criteria were ≥5 days with ≥16 h of accelerometer recordings per day, and no use of antihypertensives, diuretics or cholesterol lowering medicine. The 24-hour physical behaviour composition consisted of sedentary behaviour, standing, moving, walking, HIPA (i.e., sum of climbing stairs, running, cycling and rowing), and time in bed. We used fitted values from linear regression models to predict the difference in outcome given the investigated time reallocations relative to the group-specific mean composition.Results: Among 1053 eligible participants, we found an interaction between the physical behaviour composition and age. Age-stratified analyses (i.e., </≥65 years; 773 adults, 280 older adults) indicated that less sedentary behaviour and more walking was associated with lower SBP among older adults only. For less sedentary behaviour and more HIPA, the resultsi)indicated an association with lower SBP irrespective of age,ii)showed an association with a smaller WC among adults, andiii)showed an association with a lower LDL-C in both age groups.Conclusions: Less sedentary behaviour and more walking seems to be associated with lower CVD risk among older adults, while HIPA types are associated with lower risk among adults. Therefore, to reduce CVD risk, the modifying effect of age should be considered in future physical activity-promoting initiatives.
The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells
Authors: Lee Eun Young , Nam You Jin , Kang Sangjin , Choi Eun Ju , Han Inbo , Kim Jinwan , Kim Dong Hyun , An Ji Hae , Lee Sunghou , Lee Min Ho , Chung Ji Hyung
Published: May 2020

Background: Stress is an important cause of skin disease, including hair loss. The hormonal response to stress is due to the HPA axis, which comprises hormones such as corticotropin releasing factor (CRF) , adrenocorticotropic hormone (ACTH) , and cortisol. Many reports have shown that CRF, a crucial stress hormone, inhibits hair growth and induces hair loss. However, the underlying mechanisms are still unclear. The aim of this study was to examine the effect of CRF on human dermal papilla cells (DPCs) as well as hair follicles and to investigate whether the HPA axis was established in cultured human DPCs. Results: CRF inhibited hair shaft elongation and induced early catagen transition in human hair follicles. Hair follicle cells, both human DPCs and human ORSCs, expressed CRF and its receptors and responded to CRF. CRF inhibited the proliferation of human DPCs through cell cycle arrest at G2/M phase and induced the accumulation of reactive oxygen species (ROS) . Anagen-related cytokine levels were downregulated in CRF-treated human DPCs. Interestingly, increases in proopiomelanocortin (POMC) , ACTH, and cortisol were induced by CRF in human DPCs, and antagonists for the CRF receptor blocked the effects of this hormone. Conclusion: The results of this study showed that stress can cause hair loss by acting through stress hormones. Additionally, these results suggested that a fully functional HPA axis exists in human DPCs and that CRF directly affects human DPCs as well as human hair follicles under stress conditions.
Community health volunteers’ contribution to Tuberculosis patients notified to National Tuberculosis program through contact investigation in Kenya
Authors: Abongo Tabitha , Ulo Benson , Karanja Sarah
Published: May 2020

Background: Contact investigation is important in finding contacts of people who have Tuberculosis (TB) disease so that they can be given treatment and stop further transmission. The main objective of this study was to assess the contribution of community health volunteers (CHVs) to the number of TB patients notified to the National program in Kenya through household contact screening and referral of persons with TB signs and symptoms to the facilities for further investigation.Methods: This was a retrospective desk review of project reports submitted to Amref Health Africa in Kenya by the sub recipients implementing activities in the 33 counties with Case Notification Rate (CNR) of less 175/100,000 and Treatment Success Rate (TRS) of less than 88% as per the National strategic plan 2015-2018. Data for this study covered a time period between January and December 2016. Data on the notified TB patients was obtained from the National Tuberculosis Information Basic Unit (TIBU). The study population included all the TB index cases whose households were visited by CHVs for contact screening. Data was recorded into excel spreadsheets where descriptive analysis was done, proportions calculated and summarized in a table.Results: Community health volunteers visited a total of 26,307 TB patients (index cases) in their households for contact screening. A total of 44,617 household members were screened for TB with 43,012 (96.40%) coming from households of bacteriologically confirmed TB patients and 1,606 (3.60%) from households of children under 5 years. The proportion of the persons referred in relation to the number screened was 19.6 % for those over 5 years and 21.9 % from under 5 years with almost the same percentages for male and female at 19.2% and 19.7 % respectively. The percentage of (TB) cases identified through tracing of contacts in these counties improved to 10% (5,456) of the 54,913 cases notified to the National TB Program while the percentage of notified TB cases, all forms contributed though community referrals improved to 6 % (3,365)Conclusions: This study showed that in the 33 counties of Global Fund TB project implementation the percentage of TB cases identified through tracing of contacts improved from 6% to 10% while the percentage of notified TB cases; all forms contributed through community referrals improved from 4% to 8%. Community health volunteers can play an effective role in household contact screening and referrals for identification of TB.
Hypophosphataemia after ferric carboxymaltose is unrelated to symptoms, intestinal inflammation or vitamin D status
Authors: Fang Wendy , Kenny Rachel , Rizvi Qurat-ul-Ain , McMahon Lawrence P. , Garg Mayur
Published: May 2020

Background Intravenous iron replacement is recommended for iron-deficient patients with inflammatory bowel disease (IBD), but may be associated with hypophosphataemia, predisposing to osteomalacia and fractures. This study aimed to evaluate the incidence and risk factors for hypophosphataemia following intravenous ferric carboxymaltose (FCM) in patients with IBD.Methods This prospective observational study of patients with and without IBD evaluated serum phosphate for 28 days following intravenous FCM, and assessed associations with symptoms, markers of inflammation and vitamin D status.Results 24 patients with IBD (11 with Crohn’s disease [CD], 13 with ulcerative colitis [UC], mean age 45 years [range 19-90], 7 female), and 20 patients without IBD (mean age 56 [22-88]y, 11 female), were included. Overall, serum phosphate declined by a mean of 36% at Day 7, with a mean fall of 42% (SD 19%) at some time point over 28 days (p<0.001). Twenty-four of 44 (55%) patients developed moderate to severe hypophosphataemia (serum phosphate <0.6 mmol/L). No differences between patients with and without IBD were seen, but patients with CD had greater decline in phosphate than those with UC. There was no association between hypophosphataemia and symptomatic adverse events, faecal calprotectin, C-reactive protein, albumin, platelet count, 25(OH) vitamin D, or 1,25(di-OH) vitamin D. Serum phosphate <1.05mmol/L on Day 2 predicted susceptibility to moderate-severe hypophosphataemia (OR 7.0). Conclusions Hypophosphataemia following FCM is common, unrelated to symptomatic adverse events, baseline intestinal or systemic inflammation, or vitamin D status.
The role of community leaders on adolescent HIV and Sexual Reproductive Health and Rights in Mulanje, Malawi
Authors: Chimatiro Chancy Skenard , Hajison Precious , Muula Adamson S.
Published: May 2020

Background: We conducted this study to understand roles of community leaders on adolescents HIV and Sexual and Reproductive Health (SRH) rights in Mulanje-Malawi. We assessed how each role influence health seeking behaviour and improve SRH rights among adolescents from the local perspective. Methods: A qualitative study approach was used. We conducted 12 Focus Group Discussions (FGDs) and 17 Key Informant Interviews (KIIs) with community leaders. Purposive sampling technique was used to select study participants for both KIIs and FGDs. Random sampling technique was used to identify one Traditional Authority (TA) and 12 villages where FGDs were conducted. Inductive thematic content analysis was done guided by the study objectives to generate emerging themes. Results: Community leaders have many roles on adolescents HIV and SRH. These roles include advisory, encouragement, regulating and restricting cultural practices, formulating bye-laws and handling sexual abuse complaints. However, community leaders with religious affiliation have shown to have different views with those representing other institutions not affiliated to religion. In addition, the majority of community leaders indicated low level of knowledge on adolescent’s SRH rights. Conclusion: We suggest that the roles of community leaders differ depending on the position held and institution represented. Those community leaders not affiliated to religious can encourage certain behaviour in adolescents while those from religious background are discouraging it. Stakeholders involved in the fight against HIV and SRH rights should invest more on capacity building among the community leaders.
Tolerability and Safety of Urotainer® Polihexanide 0.02% in Long-Term Catheterized Patients: A Prospective Cohort Study
Authors: Pannek Jürgen , Everaert Karel , Möhr Sandra , Vance Will , Aa Frank Van der , Kesselring Jürg
Published: May 2020

Background: In patients with long-term indwelling bladder catheters, bacterial colonization is inevitable, leading to urinary tract infections or encrustations with subsequent catheter blockage. Currently, bladder irrigations are the most frequently used prophylactic means, but the best solution remains yet to be determined. In vitro studies demonstrate that polihexanide is a promising option for catheter irrigation, but no data about safety and tolerability exist.Methods: In a prospective observational study in patients with long-term (> 2 weeks) indwelling bladder catheter, a 0.02% polihexanide solution was used to rinse the catheter on five consecutive days. Adverse events, tolerability and vital signs were assessed before, during, after and at the end of the treatment period. Results: There was no serious adverse event in the study. A total of 28 adverse events (AEs) in 15 (46.88%) participants were experienced. Absolute changes in pain scores were not clinically relevant. No incidences of either flushing or sweating were found during instillation. Bladder spasms during instillation were reported in two cases during a single instillation. Mean pulse rates did not change by more than 3 beats per minute. Mean changes in body temperature did not exceed 0.12 °C. Clinically relevant changes in blood pressure were recorded for 3 patients.Conclusions: This is the first study to demonstrate that a 0.02% polihexanide solution can safely be used for catheter irrigation.
Levels of support for the licensing of tobacco retailers in Australia: Findings from the National Drug Strategy Household Survey 2004-2016
Authors: Baker John , Masood Mohd , Rahman Muhammad Aziz , Begg Stephen
Published: May 2020

Assessing public opinion towards tobacco policies is important, particularly when determining the possible direction of future public health policies. The aim of this study was to describe the implementation of tobacco retailer licensing systems by state and territory governments in Australia, and to use the National Drug Strategy Household Survey (NDSHS) to assess levels of public support for a retailer licensing system in each jurisdiction over time and by a range of socio-demographic and behavioural attributes. National and state/territory estimates of public support for a tobacco retailer licensing system were derived as proportions using NDSHS data from 2004 to 2016. The effect of one’s jurisdiction of residence on the likelihood of supporting such an initiative in 2016 was assessed using logistic regression while controlling for various socio-demographic and behavioural characteristics. Public support for a tobacco retailer licensing system ranged from a high of 67.2% (95% CI 66.5% – 67.9%) nationally in 2007 and declined to 59.5% (95% CI 58.9% – 60.2%) in 2016. In 2016, support was greatest amongst those from Tasmania, those aged 50 years and older, females, those from the least disadvantaged areas, those living in major cities, never-smokers and never-drinkers. After adjusting for the socio-demographic and behavioural attributes of respondents, those from Queensland were significantly less likely to support a licensing system (adjusted OR = 0.85, 95% CI 0.77 – 0.94) compared to those from other jurisdictions, while those from Tasmania were significantly more likely to support a licensing system compared to those from other jurisdictions (adjusted OR = 1.29 , 95% CI 1.09 – 1.52). A clear majority of the public support a tobacco retailer licensing system, regardless of whether or not such a system is already in place in their jurisdiction of residence. Tobacco control initiatives other than a retailer licensing system may explain some of the residual variations in support observed between jurisdictions.
Dynamics of antimalarial antibodies in non-immune patients during and after a first and unique P. falciparum malaria episode.
Authors: Bisoffi Zeno , Bertoldi Marco , Silva Ronaldo , Bertoli Giulia , Ursini Tamara , Marocco Stefania , Piubelli Chiara , Pomari Elena , Buonfrate Dora , Gobbi Federico
Published: May 2020

Background Malaria is a major travel medicine issue. Retrospective confirmation of a malaria episode diagnosed in an endemic area can have relevant implications in trasfusional medicine in Europe, where blood donors are excluded from donation on the basis of positive malaria serology. However, there is scarce evidence on the dynamics of antimalarial antibodies after a first malaria episode in non immune individuals. First aim of this study was to describe the dynamics of antimalarial antibodies in a first malaria episode in non immune travellers. Secondary objectives were to assess the sensitivity of serology for a retrospective diagnosis in non immune travellers diagnosed while abroad and to discuss the implications in transfusional medicine. Methods Retrospective analysis of the results of an indirect fluorescence antibody test (IFAT) for malaria available for patients with a first malaria episode by Plasmodium falciparum and admitted at the IRCCS Sacro Cuore Don Calabria hospital in a 14-year period. The antibody titres were collected at baseline and during further follow up visits. Epidemiological, demographic and laboratory test results (including full blood count and malaria parasite density) were anonymously recorded in a study specific electronic Case Report Form created with OpenClinica software. Statistical analysis was performed with SAS software version 9.4. Results Thirty-six patients were included. Among them, all but two were Europeans (one African and one American). Median length of fever before diagnosis was two days (IQR 1-3). Thirty-five patients had seroconversion between day 1 and day 4 from admission, and the titre showed a sharply rising titre, often to a very high level in a few days. Only a single patient remained negative in the first 5 days from admission, after which he was no more tested. Six patients were followed up for at least two months, and they all showed a decline in IFAT titre, tending to seroreversion (confirmed in one patient with the longest follow up, almost four years). Conclusions Serology demonstrated reliable for retrospective diagnosis in non immune travellers. The decline in the antimalarial titre might be included in the screening algorithms of blood donors, but further studies are needed.
Comparison of senescence-related changes between three- and two-dimensional cultured adipose-derived mesenchymal stem cells
Authors: Yin Qiliang , Xu Na , Xu Dong sheng , Dong Ming xin , Shi Xiu min , Wang Yan , Hao Zhuo , Zhu Shuang shuang , Zhao Dong hai , Jin Hao fan , Liu Wen sen
Published: May 2020

Background: Adipose-derived mesenchymal stem cells ( ADMSCs ) have attracted widespread interest as cell-based tissue repair systems. To obtain adequate quantities of ADMSCs for therapeutic applications, extensive in vitro expansion is required. However, under current two-dimensional (2D) approaches , ADMSCs rapidly undergo replicative senescence , and cell growth is impeded and stem cell properties are eliminated by mechanisms that are poorly understood . These issues limit the extensive applications of ADMSCs . In this study, we investigated senescence-related changes in mesenchymal stem cells (MSCs) isolated from human adipose tissue in 2D and three-dimensional (3D) cultures. Methods: We studied cell growth over a given period (21 days) to determine if modes of culture were associated with ADMSCs senescence . ADMSCs were isolated from healthy females by liposuction surgery and then were grew in 2D and 3D cultures. The cell morphology was observed during cell culture. Every other time of culture, senescence-associated β-galactosidase (SA-β-gal) expression, cell viability, proliferation, and differentiation potential of ADMSCs from 2D and 3D cultures were detected. Also, senescence and stemness related genes expression, telomere length, telomerase activity, and energy metabolism of ADMSCs for different culture time were evaluated. Results: With long-term propagation, we observed significant changes in cell morphology, proliferation, differentiation abilities and energy metabolism, which were associated with increases in SA-β-gal activity, and decreases in telomere length and telomerase activity . Notably, when cultured in 3D, these changes were improved. Conclusions: Our results indicate that 3D culture is able to ameliorate senescence-related changes in ADMSCs.
The association of depression and child maltreatments among Indonesian adolescents
Authors: Dhamayanti Meita , Noviandhari Anindita , Masdiani Nina , Pandia Veranita , Sekarwana Nanan
Published: May 2020

Background: Depression is one of the most prevalent emotional mental health problem among adolescents. Mental health problem might be the result of child maltreatment considering their prevalence are increasing simultaneously in Indonesia. The aim of this study is determining the association between depression and history of maltreatment among adolescents. Methods: An analytic cross-sectional study was conducted to 786 junior high school students of Bandung City, West Java, Indonesia. Subject was selected using two stage cluster sampling. The questionnaire of Children’s Depression Inventory (CDI) and ISPCAN Child Abuse Screening Tool (ICAST) were applied to assess depression and history of maltreatment respectively. Depression confirmation was diagnosed by psychiatry based on scored positive in CDI. Data were analyzed using chi-square and multiple regression test. Results: History of child maltreatment was associated with depressive disorder in adolescents (p = 0.03). All dimension of child maltreatment had significant association with depression (p <0.05). Psychological violence had the highest risk factor for the occurrence of depressive disorders (PR = 6.51), followed by violence exposure and physical violence. Sexual violence was not a common dimension of child maltreatment among students. The history of psychological violence had the strongest association with depression, which three times more likely to develop depression (POR = 3.302, p = 0.004)Conclusion: Psychological violence was proven as a strong risk factor in developing depression symptom for adolescents student. Early intervention to prevent maltreatment and its consequences is critical, as well.
Pharyngeal metastasis following living-donor liver transplantation for hepatocellular carcinoma: case report and review of literature
Authors: Tohyama Taiji , Sakamoto Katsunori , Tamura Kei , Nakamura Taro , Watanabe Jota , Wakisaka Hiroyuki , Takada Yasutsugu
Published: May 2020

Background: The most common sites of recurrence after liver transplantation for hepatocellular carcinoma (HCC) have been reported to be the liver, lung, bone, and adrenal glands, but there have also been many reports of cases of multiple recurrence. The prognosis after recurrence is poor, with reported median survival after recurrence of HCC ranging from 9 to 19 months. Here, we report a case of long-term survival after recurrence of pharyngeal metastasis following living-donor liver transplantation (LDLT) for HCC within the Milan criteria, by resection of the metastatic region and cervical lymph node dissection. Case presentation: A 47-year-old man with a Model End-stage Liver Disease (MELD) score of 11 underwent LDLT for HCC within the Milan criteria for liver cirrhosis associated with hepatitis B virus infection, with his 48-year-old elder brother as the living donor. One year and 10 months after liver transplantation, he visited a nearby hospital with a chief complaint of discomfort on swallowing. A pedunculated polyp was found in the hypopharynx, and biopsy revealed HCC metastasis. We performed pharyngeal polypectomy. Two years later, cervical lymph nodes metastasis appeared, and neck lymph node dissection was performed. Although recurrence subsequently occurred three times in the grafted liver, the patient is still alive 12 years and 10 months after recurrence of pharyngeal metastasis. He is now a tumor-free outpatient taking sorafenib. Conclusion: It is necessary to recognize that the nasopharyngeal region is a potential site of HCC metastasis. Prognostic improvement can be expected with close follow-up, early detection, and multidisciplinary treatment, including radical resection.
Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton
Authors: Zhu De , Li Ximei , Wang Zhiwei , You Chunyuan , Nie Xinhui , Sun Jie , Zhang Xianlong , Zhang Dawei , Lin Zhongxu
Published: May 2020

Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization.Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1,211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.