bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual effects of gonadotropin-inhibitory hormone on testicular development in prepubertal Minxinan Black rabbits (Oryctolagus cuniculus)
Authors: Sang Lei , Sun Shikun , Wang Jinxiang , Gao Chengfang , Chen Dongjin , Xie Xiping
Published: January 2024

Gonadotropin-inhibitory hormone (GnIH) is a neurohormone that not only suppresses reproduction at the brain level but also regulates steroidogenesis and gametogenesis at the gonad level. However, its function in gonadal physiology has received little attention in rabbits. The main objective of this study was to evaluate the effects of GnIH on testicular development and function in prepubertal Minxinan Black rabbits (Oryctolagus cuniculus). In the present study, we investigated the serum reproductive hormone concentration, testicular parameters, morphology of seminiferous tubules, apoptosis of testicular cells, and expression of reproductive-related genes in male prepubertal Minxinan Black rabbits intraperitoneally administered with 0, 0.5, 5, or 50 μg quail GnIH-related peptides (qGnIH) for 10 days. Compared with the vehicle, administration with 5 μg of qGnIH downregulated the serum testosterone concentration and mRNA levels of spermatogenic genes (PCNA, FSHR, INHβA, HSF1, and AR) and upregulated the apoptosis rate of testicular cells; administration with 50 μg of qGnIH decreased the serum testosterone concentration and hypothalamic GnIH gene mRNA level and increased the serum LH concentration, pituitary LHβ gene mRNA level, testicular weight, gonadosomatic index (GSI), and spermatogenic cell layer thickness. It is concluded that GnIH could exert dual actions on testicular development depending on the male prepubertal rabbits receiving different intraperitoneal doses.
Emerging role of extracellular vesicles in veterinary practice: novel opportunities and potential challenges
Authors: Xiong Yindi , Lou Peng , Xu Chuang , Han Bo , Liu Jingping , Gao Jian
Published: January 2024

Extracellular vesicles are nanoscale vesicles that transport signals between cells, mediating both physiological and pathological processes. EVs facilitate conserved intercellular communication. By transferring bioactive molecules between cells, EVs coordinate systemic responses, regulating homeostasis, immunity, and disease progression. Given their biological importance and involvement in pathogenesis, EVs show promise as biomarkers for veterinary diagnosis, and candidates for vaccine production, and treatment agents. Additionally, different treatment or engineering methods could be used to boost the capability of extracellular vesicles. Despite the emerging veterinary interest, EV research has been predominantly human-based. Critical knowledge gaps remain regarding isolation protocols, cargo loading mechanisms, in vivo biodistribution, and species-specific functions. Standardized methods for veterinary EV characterization and validation are lacking. Regulatory uncertainties impede veterinary clinical translation. Advances in fundamental EV biology and technology are needed to propel the veterinary field forward. This review introduces EVs from a veterinary perspective by introducing the latest studies, highlighting their potential while analyzing challenges to motivate expanded veterinary investigation and translation.
Nowe nazwy potraw, napojów i produktów spożywczych w polszczyźnie – formalno-genetyczne mechanizmy nominacyjne (rekonesans leksykograficzny
Authors: Witaszek-Samborska Małgorzata
Published: January 2024

This article is an attempt to present the formal and genetic nominative mechanisms in the new nomenclature of dishes, beverages, and foods in the Polish language. The author adopts a comparative perspective, making the description of these mechanisms in the monograph Studia nad słownictwem kulinarnym we współczesnej polszczyźnie (Studies on the culinary vocabulary in the contemporary Polish language) of 2005 the basis for her review. The contemporary material comes from two online lexicographical sources that are being developed on an ongoing basis. An analysis of the formal and genetistructure of the most recent names of dishes, beverages, and foods with the structure of the analogous lexical and semantic field from almost 20 years ago leads to the conclusion that the nominative mechanisms are characterised by flexible stability, and the predominance of the activity of the word-formation system over the importation of foreign lexemes that is observed currently (in contrast to the early 21st century) results from extralinguistic changes.
Simultaneous Calibration of European Option Volatility and Fractional Order under the Time Fractional Vasicek Model
Authors: Du Yunkang , Xu Zuoliang
Published: January 2024

In this paper, we recover the European option volatility function σ(t) of the underlying asset and the fractional order α of the time fractional derivatives under the time fractional Vasicek model. To address the ill-posed nature of the inverse problem, we employ Tikhonov regularization. The Alternating Direction Multiplier Method (ADMM) is utilized for the simultaneous recovery of the parameter α and the volatility function σ(t). In addition, the existence of a solution to the minimization problem has been demonstrated. Finally, the effectiveness of the proposed approach is verified through numerical simulation and empirical analysis.
Hybrid Rocket Engine Burnback Simulations Using Implicit Geometry Descriptions
Authors: Zeriadtke Jan Erik , Martin Joël , Wartemann Viola
Published: January 2024

The performance of hybrid rocket engines is significantly influenced by the fuel geometry. Burnback simulations, to determine the fuel surface and fluid volume, are therefore an important tool for preliminary design. This work presents a method for the simulation of spatially constant burn-ups on arbitrary geometries. An implicit surface definition by means of a signed distance function is used to represent the fluid volume and the fuel block on tetrahedral meshes. Two methods each are used to determine the fluid volume and the burning surface. The first method is based on a direct integration of the signed distance function with the Heaviside function or the Dirac delta distribution, respectively. The second method linearly interpolates the position of an isosurface and thus reconstructs the fuel surface. Both methods are compared and validated with analytical results of four example geometries. Both calculations of the fluid volume and the calculation of the surface content with the interpolation method are characterized as first-order methods. With practicable mesh resolutions of one million computational cells, errors below two percent can be achieved. With the interpolation method, numerical meshes can also be exported for any time points of the burn. Finally, the application of the program to the fuel geometry of the Viserion hybrid rocket engine is demonstrated.
RBFNN-Based Anti-Input Saturation Control for Hypersonic Vehicles
Authors: Zhang Bangchu , Liang Yiyong , Rao Shuitao , Kuang Yu , Zhu Weiyu
Published: January 2024

In hypersonic flight control, characterized by challenges posed by input saturation, model parameter uncertainties, and external disturbances, this paper introduces a pioneering anti-input saturation control method based on RBFNN adaptivity. We have developed adaptive laws to enhance control system adaptability and robustness by integrating mission profiles, actuator saturation failure modes, and self-evolving neural network design. Furthermore, our approach introduces a novel anti-input saturation auxiliary system, effectively addressing input saturation constraints. This innovation ensures system stability and precise tracking, even in severe input saturation constraints. The results reveal that the system’s steady-state tracking error remains under 2% under input saturation constraints, and the convergence speed demonstrates an impressive 20% improvement. These findings underscore this research’s substantial advancement in hypersonic flight control. It may significantly enhance the controllability and performance of hypersonic vehicles in real-world scenarios.
Estimating the Light Interception and Photosynthesis of Greenhouse-Cultivated Tomato Crops under Different Canopy Configurations
Authors: Zhang Yue , Henke Michael , Li Yiming , Sun Zhouping , Li Weijia , Liu Xingan , Li Tianlai
Published: January 2024

Understanding the spatial heterogeneity of light and photosynthesis distribution within a canopy is crucial for optimizing plant growth and yield, especially in the context of greenhouse structures. In previous studies, we developed a 3D functional-structural plant model (FSPM) of the Chinese solar greenhouse (CSG) and tomato plants, in which the greenhouse was reconstructed as a 3D mockup and implemented in the virtual scene. This model, which accounts for various environmental factors, allows for precise calculations of radiation, temperature, and photosynthesis at the organ level. This study focuses on elucidating optimal canopy configurations for mechanized planting in greenhouses, building upon the commonly used north–south (N–S) orientation by exploring the east–west (E–W) orientation. Investigating sixteen scenarios with varying furrow distance (1 m, 1.2 m, 1.4 m, 1.6 m) and row spacing (0.3 m, 0.4 m, 0.5 m, 0.6 m), corresponding to 16 treatments of plant spacing, four planting patterns (homogeneous row, double row, staggered row, incremental row) and two orientations were investigated. The results show that in Shenyang city, an E–W orientation with the path width = 0.5 (furrow distance + row distance) = 0.8 m (homogeneous row), and a plant distance of 0.32 m, is the optimal solution for mechanized planting at a density of 39,000 plants/ha. Our findings reveal a nuanced understanding of how altering planting configurations impacts the light environment and photosynthesis rate within solar greenhouses. Looking forward, these insights not only contribute to the field of CSG mechanized planting, but also provide a basis for enhanced CSG planting management. Future research could further explore the broader implications of these optimized configurations in diverse geographic and climatic conditions.
The Probiotic Potential and Metabolite Characterization of Bioprotective Bacillus and Streptomyces for Applications in Animal Production
Authors: Evangelista Alberto Gonçalves , Nazareth Tiago de Melo , Luz Carlos , Dopazo Victor , Moreno Ana , Riolo Mario , Meca Giuseppe , Luciano Fernando Bittencourt
Published: January 2024

Probiotics are increasingly recognized for their potential in managing bacterial challenges in animal production. This study aimed to evaluate the probiotic potential of Bacillus and Streptomyces strains, specifically their bioprotective ability against Salmonella. In agar inhibition assays, these bacteria supported Salmonella-inhibition zones, ranging from 2.5 ± 0.5 to 6.3 ± 2.0 mm. Analyses of antimicrobial metabolites revealed their capacity to produce compounds with anti-Salmonella properties, except for Bacillus subtilis MLB2. When Salmonella was exposed to lyophilized metabolites, inhibition occurred in both liquid (at concentrations between 250 and 500 g/L) and solid cultures (at 500 g/L). To confirm their probiotic potential, the S. griseus and Bacillus strains underwent evaluations for antimicrobial resistance, bile salt tolerance, auto- and co-aggregation, pH resistance, and their ability to adhere to and inhibit Salmonella in Caco-2 cells. These assessments confirmed their probiotic potential. The probiotic strains were further encapsulated and subjected to simulated swine and poultry digestion. They demonstrated survival potential through the gastrointestinal tract and significantly reduced the Salmonella population. Thus, these strains exhibit considerable promise for producing biotechnological products aimed at controlling Salmonella in animal production. This approach ensures the health and hygiene of farming facilities, mitigates the spread of zoonotic bacteria, and contributes positively to public health.
Agreement between Clinical Assessment and Laboratory Diagnosis of Ringworm in Calves at Auction Markets
Authors: Spergser Joachim , Neuhuber Thiemo , Haupt Herfried , Kaltenegger Gerd , Wittek Thomas
Published: January 2024

To limit the spread of bovine ringworm, control measures such as movement restrictions are highly recommended. In this context, calves at auction markets in Styria, Austria, displaying skin lesions characteristic for bovine ringworm, are excluded from the auctions. To investigate whether these clinical assessments correspond to laboratory diagnosis, a total of 166 samples taken from skin lesions assigned to the three clinical categories ‘ringworm very likely (v), likely (l) or unlikely (u)’ were mycologically examined using microscopy, culture, and nested PCR followed by amplicon sequencing. Further, the relationships of isolated dermatophytes were determined through multi-locus sequence typing (MLST). Overall, a high agreement between clinical assessment and laboratory results were observed with microscopy and nested PCR, providing more consistent results and molecular detection possessing an analytical sensitivity superior to that of cultural isolation (culture 21.7% vs. nested PCR 48.2%). Phylogenetic analyses revealed that most of the isolated dermatophytes belong to a unique Trichophyton verrucosum MLST genotype. In conclusion, clinical assessments were largely confirmed through laboratory diagnosis with nested PCR and sequencing, providing rapid, sensitive, and species-specific detection of dermatophytes in calves at auction markets displaying skin lesions typical for ringworm; this seems to be predominantly caused by a single Trichophyton verrucosum strain.
Evaluation of Extended Storage of Swine Complete Feed for Inactivation of Viral Contamination and Effect on Nutritional, Microbiological, and Toxicological Profiles
Authors: Gebhardt Jordan T. , Dee Scott A. , Little Erin , Scales Brittney N. , Kern Doug R.
Published: January 2024

The extended storage of feed ingredients has been suggested as a method to mitigate the risk of pathogen transmission through contaminated ingredients. To validate the approach of extended storage of complete swine feed for the inactivation of swine viruses, an experiment was conducted wherein swine feed was inoculated with 10 mL of 1 × 105 TCID50/mL of porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and Senecavirus A (SVA) and stored for 58 d at 23.9 °C. Measures of feed quality were also evaluated at the initiation and conclusion of the storage period including screening for mycotoxins, characterization of select microbiological measures, and stability of phytase and dietary vitamins. Storing feed for 58 d under either ambient or anaerobic and temperature-controlled storage conditions did not result in substantial concerns related to microbiological profiles. Upon exposure to the feed following 58 d of storage in a swine bioassay, previously confirmed naïve pigs showed no signs of PEDV or SVA replication as detected by the PCR screening of oral fluids and serum antibody screening. Infection with SVA was documented in the positive control room through diagnostic testing through the State of Minnesota. For PRRSV, the positive control room demonstrated infection. For rooms consuming inoculated feed stored for 58 d, there was no evidence of PRRSV infection with the exception of unintentional aerosol transmission via a documented biocontainment breach. In summary, storing feed for 58 d at anaerobic and temperature-controlled environmental conditions of 23.9 °C validates that the extended storage of complete swine feed can be a method to reduce risks associated with pathogen transmission through feed while having minimal effects on measures of nutritional quality.
Health Effects of Electrolyzed Hydrogen Water for the Metabolic Syndrome and Pre-Metabolic Syndrome: A 3-Month Randomized Controlled Trial and Subsequent Analyses
Authors: Moribe Reiko , Minami Marina , Hirota Ryoji , J-P Naw Awn , Kabayama Shigeru , Eitoku Masamitsu , Yamasaki Keiko , Kuroiwa Hajime , Suganuma Narufumi
Published: January 2024

We studied the effect of three months’ use of electrolyzed hydrogen water (EHW, Electrolyzed Hydrogen Water conditioner produced by Nihon Trim Co., Ltd.) on metabolic and pre-metabolic syndrome groups. This research was carried out jointly by Susaki City; Nihon Trim Co., Ltd.; and Kochi University as part of a local revitalization project with health as a keyword. A randomized, placebo-controlled, double-blind, parallel-group trial was conducted to evaluate the clinical impact of EHW on participants who suffered from metabolic syndrome or pre-metabolic syndrome. EHW was produced via electrolysis using a commercially available apparatus (Nihon Trim Co., Ltd., Osaka, Japan). During exercise, oxidative stress increases, and active oxygen species increase. In this study, we examined 181 subjects with metabolic syndrome or pre-metabolic syndrome. Among the group that drank EHW for 3 months, those who also engaged in a high level of physical activity showed a significant difference in waist circumference reduction. Although no significant difference was observed, several positive results were found in the participants who engaged in a high level of physical activity. Urinary 8-OHdG, urinary nitrotyrosine, HbA1c, and blood glucose levels increased in the filtered water (FW) group but decreased in the EHW group. High-sensitivity CRP increased less in the EHW group. 8-Isoprostane decreased more in the EHW group. In subgroup analysis, the EHW group showed a significantly greater reduction in waist circumference than the FW group only when controlled for high physical activity. Based on the result, we suggest that, among participants in the study who suffered from metabolic syndrome and pre-metabolic syndrome in which the level of active oxygen species is said to be higher than in healthy subjects, the group that consumed EHW and also engaged in a high level of physical activity experienced a suppressed or reduced increase in active oxygen species.
Effect of Digested Selected Food Items on Markers of Oxidative Stress and Inflammation in a Caco-2-Based Human Gut Epithelial Model
Authors: Vahid Farhad , Krischler Pit , Leners Bernadette , Bohn Torsten
Published: January 2024

The human gut epithelium presents a crucial interface between ingested food items and the host. Understanding how different food items influence oxidative stress and inflammation in the gut is of great importance. This study assessed the impact of various digested food items on oxidative stress, inflammation, and DNA/RNA damage in human gut epithelial cells. Differentiated Caco-2 cells were exposed to food items and their combinations (n = 22) selected from a previous study, including sausage, white chocolate, soda, coffee, orange juice, and curcumin. Following stimulation with TNF-α/IFN-1β/LPS and H2O2 for 4 h, the cells were exposed to digested food items or appropriate controls (empty digesta and medium) for a further 16 h. Cell viability, antioxidant capacity (ABTS, FRAP), IL-6, IL-8, F2-isoprostanes, lipid peroxidation (MDA), and DNA/RNA oxidative damage were assessed (3 independent triplicates). The ABTS assay revealed that cells treated with “white chocolate” and “sausage + coffee” exhibited significantly reduced antioxidant capacity compared to stimulated control cells (ABTS = 52.3%, 54.8%, respectively, p < 0.05). Similar results were observed for FRAP (sausage = 34.9%; white chocolate + sausage = 35.1%). IL-6 levels increased in cells treated with “white chocolate + sausage” digesta (by 101%, p < 0.05). Moreover, MDA levels were significantly elevated in cells treated with digested “sausage” or sausage in combination with other food items. DNA/RNA oxidative damage was found to be higher in digesta containing sausage or white chocolate (up to 550%, p < 0.05) compared to stimulated control cells. This investigation provides insights into how different food items may affect gut health and underscores the complex interplay between food components and the epithelium at this critical interface of absorption.
Protective Effects of Four Structurally Distinct Sanshools Ameliorate Dextran Sodium Sulfate-Induced Ulcerative Colitis by Restoring Intestinal Barrier Function and Modulating the Gut Microbiota
Authors: Chen Zhaojun , Wang Hui , Tan Lulin , Liu Xiong
Published: January 2024

Hydroxy-α-sanshool (HAS), hydroxy-β-sanshool (HBS), hydroxy-γ-sanshool (HRS), and γ-sanshool (RS) are the key components from the Zanthoxylum genus, processing a range of pharmacological activities. The present study investigated the protective capacities of four sanshools on a dextran sulfate sodium (DSS)-induced model of ulcerative colitis (UC). The results showed that sanshool administration alleviated the colitis symptoms by reducing body weight loss and disease activity index (DAI) score, increasing the colon length, and improving colonic injury and the change in immune organ weight. Furthermore, sanshools enhanced the antioxidant enzyme activities, and RS exhibited the lowest effect on the improvement in total antioxidative capacity (T-AOC) and antioxidant abilities compared to the other three sanshools. The p65 nuclear factor κB (p65 NFκB) signaling pathway was inhibited to prevent hyperactivation and decreased the production of inflammatory factors. The gut barrier function in DSS-induced mice was restored by increasing goblet cell number and levels of tight junction proteins (zonula occludens-1, occludin, and claudin-1), and the levels of protein in HAS and HRS groups were higher than that in the HBS group, significantly. The analysis of gut microbiota suggested that sanshool administration significantly boosted the abundance of Lachnospiraceae, Muribaculaceae, Oscillospiraceae, and Alistipes and reduced the level of Buchnera in colitis mice. Collectively, the sanshool treatment could ameliorate colitis by resisting colon injury and regulating intestinal barrier dysfunction and gut microbiota dysbiosis; meanwhile, HRS and HAS have better improvement effects.
Digital Twins in Software Engineering—A Systematic Literature Review and Vision
Authors: Guinea-Cabrera Miguel A. , Holgado-Terriza Juan A.
Published: January 2024

Digital twins are a powerful consequence of digital transformation. In fact, they have been applied to many industries to enhance operations, predict needs, improve decision making, or optimize performance, even though the definition of digital twins is still evolving. However, their impact on the software industry is still limited. Thus, this work aims to analyze the current adoption of digital twins in the software industry as a potential path to integrate them into application lifecycle management. To achieve this objective, first, the significant characteristics of current digital twins are analyzed in their application to manufacturing to understand how the knowledge and the lessons learned can be transferred to the software industry. Second, a systematic literature review was conducted on Scopus, the Web of Science, and the ScienceDirect database. The literature review revealed 93 documents after data screening and cleaning 251 initial documents. Our main findings are that digital twins are already influencing and will significantly affect the software industry, revolutionizing various aspects of the software development lifecycle. This study tackles what identifies a digital twin in the software industry, the specific domains and areas where they can be applied in the software lifecycle, and the proposed approaches explored to build digital twins for developing, deploying, and maintaining software systems. Finally, this study proposes some guidelines for building digital twins in the context of application lifecycle management. Determining an appropriate roadmap shortly is essential to achieve a widespread applicability to building suitable digital twins and preparing organizations for the software industry.
Emergent and Known Honey Bee Pathogens through Passive Surveillance in the Republic of Kosovo
Authors: Hulaj Beqë , Granato Anna , Bordin Fulvio , Goga Izedin , Merovci Xhavit , Caldon Mauro , Cana Armend , Zulian Laura , Colamonico Rosa , Mutinelli Franco
Published: January 2024

In recent years, honey bee colony losses in the Republic of Kosovo remained largely unknown. From 2019 to 2021, 81 apiaries with different disease suspicions were investigated in the framework of honey bee disease passive surveillance. Fifty-nine of the eighty-one apiaries were tested for Vairimorpha ceranae, Vairimorpha apis, trypanosomatids Lotmaria passim, and Crithidia mellificae. All samples were positive for V. ceranae (100%) whereas L. passim was found with a lower frequency (11.9%). V. apis and C. mellificae were not found. Thirteen of the eighty-one apiaries were tested for seven viruses (ABPV, CBPV, DWV, BQCV, SBV, IAPV, KBV) and five of them were found (ABPV, CBPV, DWV, BQCV, SBV). The most frequently detected viruses in honey bees and Varroa mites were DWV (100%) followed by BQCV, ABPV, SBV, and CBPV (92.3%, 69.2%, 30.8%, and 7.7%, respectively). Varroa mite samples had different degrees of co-infection by viruses. Nine of the eighty-one apiaries consisted of brood combs with larvae, eight of them were AFB positive, ERIC I genotype, and one EFB positive. This paper represents the first molecular investigation (PCR) and detection of the honey bee viruses ABPV, CBPV, DWV, BQCV, and SBV as well as V. ceranae, L. passim, and M. plutonius in the Republic of Kosovo.
Exploring Heuristic and Optimization Approaches for Elevator Group Control Systems
Authors: Gharbi Atef
Published: January 2024

This paper undertakes an examination of elevator car dispatching methods in response to hall calls. Firstly, our study focuses on the establishment of a representation of an elevator group control system as a finite-state machine to understand the dynamics of elevator group control. Secondly, two primary heuristics are explored, with the first advocating directional continuity unless the highest or lowest floor has been reached, while the second permits direction change upon completing the final call, regardless of floor extremes. Identified inefficiencies in these heuristic solutions lead us to explore enhanced alternatives. Consequently, we delve into genetic algorithm (GA) and simulated annealing (SA) methodologies. Our focus initially centers on devising solution representations and determining fitness evaluations for both approaches. We employ a simulation-based optimization approach to identify the optimal parameter values for both simulated annealing and genetic algorithms. A subsequent comparative analysis is conducted to ascertain the most effective approach among these diverse solutions. A comparative analysis reveals that the GA-based approach significantly outperforms both existing heuristics and the SA-based method in minimizing average passenger waiting time at the cost of longer computational time.
Assessment of Cyber-Physical Inverter-Based Microgrid Control Performance under Communication Delay and Cyber-Attacks
Authors: Ali Ola , Nguyen Tung-Lam , Mohammed Osama A.
Published: January 2024

The integration of communication infrastructures into traditional power systems, transforming them into cyber-physical power systems (CPPS), accentuates the significance of communication in influencing system performance and sustainability. This paper presents a versatile, innovative cyber-physical co-simulation framework that integrates the physical power system and communication networks, uniting OPAL-RT, a network simulator (ns3), and Docker containers into a sophisticated platform, facilitating intensive studies into CPPS dynamics. The proposed experimental study provides an innovative way to assess the frequency control response of a cyber-physical inverter-based microgrid (MG), addressing the MG sustainability challenges. We consider diverse real-world scenarios, focusing on communication delays and distributed denial of service (DDoS) attacks within the communication channels. We propose a precise ns3-based communication model that bridges the MG’s primary and secondary control layers, an aspect often overlooked in previous studies; this is a noteworthy contribution to elucidating the adverse impacts of communication latency on MG frequency performance. The experimental results demonstrate the effectiveness of the centralized secondary controller in eliminating the frequency deviations. Furthermore, the findings offer insights into stable and unstable regions, revealing how the communication delay value affects the frequency stability under different operating conditions. In addition, the developed real-time DDoS attacks model within the proposed communication surface unveils crucial insights into the MG’s resilience to cyber threats. This work’s revelations offer a foundational awareness of MG vulnerabilities, paving the way for designing robust and resilient communication networks and control strategies within the cyber-physical inverter-based microgrids.
Gear Integrated Error Determination Using the Gaussian Template Convolution-Facet Method
Authors: Fang Yiming , Shi Zhaoyao , Sun Yanqiang , Zhang Pan
Published: January 2024

A gear integrated error, a combination of individual and composite errors, carries richer information and has long been a key target of classic gear error measurement techniques. However, in the age of intelligent manufacturing, the classic methods for gear integrated error measurement are no longer able to meet the emerging requirements of large-scale gears and real-time online measurement. To address this gap, a novel approach to obtaining the gear integrated error based on GTC−Facet (Gaussian template convolution-Facet) is proposed. This method accurately pinpoints the sub-pixel contour of gears in images, enabling a quick derivation of the gear integrated error curve. From this curve, other individual and composite errors can be analyzed. The gear error information obtained through our method has higher measurement accuracy, achieving a positioning accuracy of 3.6 μm for the gear profile. Moreover, during the measurement process, the measured gear remains unclamped, and the entire measurement process can be completed within 0.35 s, which is much faster than classic methods. Our method meets the demands of online measurements and provides a new avenue for gear error measurement.
Identification of Adulterants in Chili Powder Based on the Histogram of Oriented Gradients Algorithm by Using an Electronic Nose
Authors: Peng Peng , Ba Fushuai , Zhang Yafei , Jiang Feiyang , Zhao Yongli
Published: January 2024

Driven by economic interests, adding adulterations in chili powder is a problem which threatens people’s health. Thanks to its nondestructive, rapid, and portable advantages, electronic nose has more potential to be used for adulteration detection than the traditional methods. An approach for identifying the adulterants in chili powder was proposed in this paper. Firstly, an electronic nose system with 10 gas sensors was designed, and then the response images were drawn based on the response signals of the electronic nose. Afterwards, gas features were extracted from those response images by using a histogram of oriented gradients (HOG) algorithm. Finally, an SVM-based identification model was constructed to achieve the identification of plant adulterants in chili powder. The experimental results showed that the identification accuracy of the adulterant categories (almond shell, red beetroot, and tomato peel) based on the HOG features could reach up to 98.3%, and the identification results for adulterant content were 94.2%, 93.3%, and 95%, respectively. Furthermore, in order to compare the efficiency of the proposed identification approach, the widely used model AlexNet was also investigated and discussed.
Date-Palm-Based Sustainable Hybrid Composite with Cotton and Kevlar Fibre Participation
Authors: Zeleniuc Octavia , Mazaherifar Mohammad Hassan , Coșereanu Camelia , Suciu Andrei
Published: January 2024

This research aims to evaluate the physical and mechanical performance of three types of hybrid composites made of date palm (Phoenix dactylifera L.) (DP), additional layers of cotton (DP/C) and Kevlar fibres (DP/K). The fibres were formed into flat sheets and employed as reinforcement layers embedded in a polyester matrix. Three-layer and five-layer hybrid composites were created using the hand layup method. The layers have alternative longitudinal–transversal orientation. The composites were investigated for density, thickness swelling (TS), water absorption (WA), flexural strength and modulus of elasticity (MOE) properties. Moreover, the composites were subjected to cycles of water immersion, freezing and drying, and the changes in mass and mechanical performance were analysed before and after the cyclic testing. The hybrid composite with Kevlar as the inner layers displayed better physical and mechanical properties when compared to the other two hybrid composites. A stereo-microscopic investigation revealed that poor adhesion between the layers of composites contributed to a reduction in the mechanical properties of DP/C and DP hybrid composites. The DP/C composite had the highest thickness swelling and water absorption, with the water uptake more pronounced than in the cases of the other composites. The hybridisation of date palms with Kevlar fibres improved the properties of the hybrid composites.
Potnia’s Participants: Considering the Gala, Assinnu, and Kurgarrû in an Aegean Context
Authors: Pareja Marie N.
Published: January 2024

The wall paintings from the site of Akrotiri, Thera, are often considered to be instrumental to understanding elements of life in the Bronze Age. This is partially due to their high degree of preservation. The large-scale detail present in the scenes allows for a more detailed and nuanced understanding of the imagery that survives in glyptic art that, considered together with the surviving wall paintings, helps to better inform one’s understanding of Aegean life. Many of the iconographic elements and themes, however, remain at least partially enigmatic. This is particularly the case for Xeste 3, a cultic building at Akortiri, where the wall paintings contribute to a larger, programmatic cultic narrative. The current investigation seeks to better understand the monkeys scene from Room 2 of the first floor by deconstructing and examining each visual element via comparative analyses. They are first contextualized within the Aegean, then considered in light of Mesopotamian comparanda. This method allows for possible parallels between the monkeys from Xeste 3 and at least three priestly classes known from contemporary Mesopotamian tradition: the gala, assinnu, and kurgarrû. Each of these priestly classes belonged to the adaptable and widespread cult of Inanna, one of the most powerful and popular deities in Mesopotamia.
ECMWF Lightning Forecast in Mainland Portugal during Four Fire Seasons
Authors: Campos Cátia , Couto Flavio T. , Santos Filippe L. M. , Rio João , Ferreira Teresa , Salgado Rui
Published: January 2024

The study evaluated the ECMWF model ability in forecasting lightning in Portugal during four fire seasons (2019–2022). The evaluation was made based on lightning data from the national lightning detector network, which was aggregated into resolutions of 0.5° and 1° over 3 h periods and analyzed from statistical indices using two contingency tables. The results showed that the model overestimates the lightning occurrence, with a BIAS greater than 1, with a success rate of 57.7% (49%) for a horizontal resolution of 1° (0.5°). The objective analysis was complemented by the spatial lightning distribution analysis, which indicated a time lag between the two data, i.e., the model started predicting lightning before its occurrence and finished the prediction earlier. Furthermore, such analysis revealed the lightning distribution being consistent with some weather patterns. The findings of this study provide insights into the applicability of the ECMWF lightning forecast data in the context of forecasting natural forest fires in Portugal.
Role of Mitochondrial ROS for Calcium Alternans in Atrial Myocytes
Authors: Oropeza-Almazán Yuriana , Blatter Lothar A.
Published: January 2024

Atrial calcium transient (CaT) alternans is defined as beat-to-beat alternations in CaT amplitude and is causally linked to atrial fibrillation (AF). Mitochondria play a significant role in cardiac excitation–contraction coupling and Ca signaling through redox environment regulation. In isolated rabbit atrial myocytes, ROS production is enhanced during CaT alternans, measured by fluorescence microscopy. Exogenous ROS (tert-butyl hydroperoxide) enhanced CaT alternans, whereas ROS scavengers (dithiothreitol, MnTBAP, quercetin, tempol) alleviated CaT alternans. While the inhibition of cellular NADPH oxidases had no effect on CaT alternans, interference with mitochondrial ROS (ROSm) production had profound effects: (1) the superoxide dismutase mimetic MitoTempo diminished CaT alternans and shifted the pacing threshold to higher frequencies; (2) the inhibition of cyt c peroxidase by SS-31, and inhibitors of ROSm production by complexes of the electron transport chain S1QEL1.1 and S3QEL2, decreased the severity of CaT alternans; however (3) the impairment of mitochondrial antioxidant defense by the inhibition of nicotinamide nucleotide transhydrogenase with NBD-Cl and thioredoxin reductase-2 with auranofin enhanced CaT alternans. Our results suggest that intact mitochondrial antioxidant defense provides crucial protection against pro-arrhythmic CaT alternans. Thus, modulating the mitochondrial redox state represents a potential therapeutic approach for alternans-associated arrhythmias, including AF.
Silent Myocardial Ischemia: From Pathophysiology to Diagnosis and Treatment
Authors: Theofilis Panagiotis , Antonopoulos Alexios , Sagris Marios , Papanikolaou Aggelos , Oikonomou Evangelos , Tsioufis Konstantinos , Tousoulis Dimitris
Published: January 2024

Silent myocardial ischemia (SMI), characterized by a lack of overt symptoms despite an inadequate blood supply to the myocardium, remains a challenging entity in cardiovascular medicine. The pathogenesis involves intricate interactions of vascular, neurohormonal, and metabolic factors, contributing to perfusion deficits without the characteristic chest pain. Understanding these mechanisms is pivotal for recognizing diverse clinical presentations and designing targeted interventions. Diagnostic strategies for SMI have evolved from traditional electrocardiography to advanced imaging modalities, including stress echocardiography, single-photon emission computed tomography (SPECT), positron emission tomography (PET), and cardiac magnetic resonance imaging (MRI). Treating SMI is a matter of ongoing debate, as the available evidence on the role of invasive versus medical management is controversial. This comprehensive review synthesizes current knowledge of silent myocardial ischemia, addressing its pathophysiology, diagnostic modalities, and therapeutic interventions.
A Systematic Review and Meta-Analysis of Mean Platelet Volume and Platelet Distribution Width in Patients with Obstructive Sleep Apnoea Syndrome
Authors: Di Lorenzo Biagio , Scala Chiara , Mangoni Arduino A. , Zoroddu Stefano , Paliogiannis Panagiotis , Pirina Pietro , Fois Alessandro G. , Carru Ciriaco , Zinellu Angelo
Published: January 2024

Obstructive sleep apnoea syndrome (OSAS) is a highly prevalent yet underestimated disorder caused by the complete or partial obstruction of the upper airways. Although polysomnography is the gold standard for OSAS diagnosis, there is an active search for easily accessible biomarkers of disease presence and severity, particularly those reflecting morphological changes in specific blood cells. We investigated the associations between the presence and severity of OSAS, continuous positive airway pressure (CPAP) treatment, mean platelet volume (MPV), and platelet distribution width (PDW), routinely assessed as part of the complete blood count. From 262 retrieved records from PubMed, the Web of Science, Scopus, and Google Scholar, 31 manuscripts were selected for a final analysis, 30 investigating MPV and 15 investigating PDW. MPV was not statistically different between OSAS patients and healthy controls; however, it progressively increased with disease severity. By contrast, OSAS patients had significantly higher PDW values than controls (SMD = 0.40, 95% CI: 0.25 to 0.56; p ˂ 0.001), and the difference increased with disease severity. In a univariate meta-regression, there were significant associations between the MPV and publication year, the apnoea–hypopnea index, and diabetes mellitus, while no associations were observed with the PDW. No significant between-group differences were observed in the subgroup analyses. These data suggest that PDW, and to a lesser extent, MPV, are potential biomarkers of OSAS and require further research to ascertain their pathophysiological significance (PROSPERO, CRD42023459413).
Do Double-Expressor High-Grade B-Cell Lymphomas Really Need Intensified Treatment? A Report from the Real-Life Series of High-Grade B-Cell Lymphomas Treated with Different Therapeutic Protocols at the Institute of Oncology Ljubljana
Authors: Boltežar Lučka , Rožman Samo , Gašljević Gorana , Grčar Kuzmanov Biljana , Jezeršek Novaković Barbara
Published: January 2024

High-grade B-cell lymphomas with MYC and BCL2 and/or BCL6 rearrangements are known for their aggressive clinical course and so are the ones with MYC and BCL2 protein overexpression. The optimal therapy for these lymphomas remains to be elucidated. A retrospective analysis of all diffuse large B-cell lymphomas and high-grade B-cell lymphomas with MYC and BCL2 and/or BCL6 rearrangements diagnosed between 2017 and 2021 at the Institute of Oncology Ljubljana, Slovenia, has been performed. Only patients with double-expressor lymphoma (DEL), double-hit lymphoma (DHL), or triple-hit lymphoma (THL) were included. Demographic and clinical parameters were assessed, as well as progression-free survival (PFS) and overall survival (OS). In total, 161 cases out of 309 (161/309; 52,1%) were classified as DEL. Sixteen patients had DHL, MYC/BCL2 rearrangement was observed in eleven patients, and MYC/BCL6 rearrangement was observed in five patients. Five patients were diagnosed with THL. Out of 154 patients (according to inclusion/exclusion criteria) included in further evaluation, one-hundred and thirty-five patients had double-expressor lymphoma (DEL), sixteen patients had DHL, and three patients had THL. In total, 169 patients were treated with R-CHOP, 10 with R-CHOP and intermediate-dose methotrexate, 19 with R-DA-EPOCH, and 16 with other regimens. The median follow-up was 22 months. The 5-year OS for the whole DEL group was 57.1% (95% CI 45.9–68.3%) and the 5-year PFS was 76.5% (95% CI 72.6–80.4%). The log-rank test disclosed no differences in survival between treatment groups (p = 0.712) while the high-risk international prognostic index (IPI) carried a significantly higher risk of death (HR 7.68, 95% CI 2.32–25.49, p = 0.001). The 5-year OS for DHL patients was 32.4% (95% CI 16.6–48.2%) while all three TH patients were deceased or lost to follow-up. Our analyses of real-life data disclose that the R-CHOP protocol with CNS prophylaxis is a successful and curative treatment for a substantial proportion of DEL patients.
A Comparison of Normalization Techniques for Individual Baseline-Free Estimation of Absolute Hypovolemic Status Using a Porcine Model
Authors: Lambert Tamara P. , Chan Michael , Sanchez-Perez Jesus Antonio , Nikbakht Mohammad , Lin David J. , Nawar Afra , Bashar Syed Khairul , Kimball Jacob P. , Zia Jonathan S. , Gazi Asim H. , Cestero Gabriela I. , Corporan Daniella , Padala Muralidhar , Hahn Jin-Oh , Inan Omer T.
Published: January 2024

Hypovolemic shock is one of the leading causes of death in the military. The current methods of assessing hypovolemia in field settings rely on a clinician assessment of vital signs, which is an unreliable assessment of hypovolemia severity. These methods often detect hypovolemia when interventional methods are ineffective. Therefore, there is a need to develop real-time sensing methods for the early detection of hypovolemia. Previously, our group developed a random-forest model that successfully estimated absolute blood-volume status (ABVS) from noninvasive wearable sensor data for a porcine model (n = 6). However, this model required normalizing ABVS data using individual baseline data, which may not be present in crisis situations where a wearable sensor might be placed on a patient by the attending clinician. We address this barrier by examining seven individual baseline-free normalization techniques. Using a feature-specific global mean from the ABVS and an external dataset for normalization demonstrated similar performance metrics compared to no normalization (normalization: R2 = 0.82 ± 0.025|0.80 ± 0.032, AUC = 0.86 ± 5.5 × 10−3|0.86 ± 0.013, RMSE = 28.30 ± 0.63%|27.68 ± 0.80%; no normalization: R2 = 0.81 ± 0.045, AUC = 0.86 ± 8.9 × 10−3, RMSE = 28.89 ± 0.84%). This demonstrates that normalization may not be required and develops a foundation for individual baseline-free ABVS prediction.
Digital PCR for Single-Cell Analysis
Authors: Fang Weibo , Liu Xudong , Maiga Mariam , Cao Wenjian , Mu Ying , Yan Qiang , Zhu Qiangyuan
Published: January 2024

Single-cell analysis provides an overwhelming strategy for revealing cellular heterogeneity and new perspectives for understanding the biological function and disease mechanism. Moreover, it promotes the basic and clinical research in many fields at a single-cell resolution. A digital polymerase chain reaction (dPCR) is an absolute quantitative analysis technology with high sensitivity and precision for DNA/RNA or protein. With the development of microfluidic technology, digital PCR has been used to achieve absolute quantification of single-cell gene expression and single-cell proteins. For single-cell specific-gene or -protein detection, digital PCR has shown great advantages. So, this review will introduce the significance and process of single-cell analysis, including single-cell isolation, single-cell lysis, and single-cell detection methods, mainly focusing on the microfluidic single-cell digital PCR technology and its biological application at a single-cell level. The challenges and opportunities for the development of single-cell digital PCR are also discussed.