bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Seaweed Flour (Kappaphycus alvarezii) as an Innovation in the Manufacture of High Fiber Fettucine Paste Products
Authors: Pramestika D , Pujiastuti D Y , Patmawati
Published: July 2022

Fettucine is one type of pasta that is generally made from wheat flour with a mixture of eggs and water. Wheat flour is rich in carbohydrates and low in fiber can cause obesity. One of the alternatives to overcome this problem is to increase fiber consumption by utilizing fishery commodities such as seaweed to be the main ingredient in making fettucine, namely Kappaphycus alvarezii seaweed flour. This study used experimental laboratory research methods to determine the effect of adding Kappaphycus alvarezii seaweed flour as a source of fiber to the nutritional value and organoleptic characteristics of fettucine. The design used was a completely randomized design (CRD) with one factor, consisting of 4 concentration treatments and 3 replications. The results showed that there was a significant difference between treatments (P<0.05). The highest nutritional value is obtained from the addition of 50% containing water (26,18%), ash (3,58%), protein (3,04%), fat (5,83%), fiber (3,29%), and carbohydrate (58,08%). Meanwhile, organoleptic test results with hedonic parameters showed the best treatment at the addition of 20% with an average color value (6,50%), aroma (6,77%), texture (7,17%), and taste (6,87%).
Quality Enhancement in Seaweed Dodol Using Edible Film Carrageenan Packaging
Authors: Savitri N A , Masithah E D , Tjahjaningsih W
Published: July 2022

The addition of seaweed raw materials to produce a varied taste of dodol, the texture of dodol is softer and rich in fiber, especially soluble fiber. To maintain the quality of dodol with edible film packaging materials, it can reduce packaging waste, maintain aroma and appearance, and prevent contamination and microorganisms. The purpose of this study was to determine the effect of the application of carrageenan edible film packaging used on the quality of seaweed dodol. This study used a completely randomized design (CRD) with three treatments and four replications. The parameters tested were the measurement of the total number of bacteria, water content, and organoleptic. Data were analyzed using ANOVA test and continued with Duncan's test. The results showed that packaging with edible film carrageenan was able to maintain the total number of bacteria in dodol according to SNI until the 48th hour at room temperature storage, which was less than 5.0x105 colonies/g. The total number of bacteria in the seaweed dodol packaged in edible film was 1.61x105 colonies/g, which was lower than the seaweed dodol using baking paper packaging. The packaging of seaweed dodol using edible film can maintain the sensory value of the product for up to three days at room temperature.
Analysis of Blood Glucose Levels and The Development of Ectoparasite Infestation on Pacific White Shrimp (Litopenaeus vannamei) Which Were Given Crude Protein Zoothamnium penaei at High Stocking Densities
Authors: Mahasri G , Satyantini W H , Mukti A T
Published: July 2022

The main constraint in pacific white shrimp culture with high stocking density (super intensive), can cause a decrease in water quality and shrimp were stress and are susceptible to disease. The purpose of this study was to analyze stress levels (blood glucose levels) and the development of ectoparasite infestations. In this experimental study, used 2 ponds with an area of 2000 m2 per pond, stocking densities in pond I were 150 individuals/m2 and 350 individuals/ m2 in pond II, with a maintenance period of 90 days. The results showed that the shrimp was stressed during rearing, with blood glucose levels of white shrimp at a high stocking density of 350 individuals/ m2 ranging from 43-54 mg/dL significantly different from blood glucose levels at stocking density of 150 individuals/ m2 (p<0.05). The ectoparasites found were the genera Zoothamnium, Epistylis and Vorticella. The development of the infestation of the three genera showed an increase starting on the 30th, 60th and 90th days, the degree of infestation from mild to moderate, but on the 90th day there was a significant difference (p<0.05), with a moderate degree of infestation in the stocking density. 100 individuals/m2 and weight at stocking density 350 individuals/ m2.
Mitigation and adaptation to climate change through sustainable mangrove management on the coast of Rembang Regency
Authors: Sutanto H A , Susilowati I , Iskandar D D , Waridin
Published: July 2022

Climate change is very influential on the mangrove ecosystem. Adaptation and mitigation are urgent needs to minimize the impact of climate change. This study aims to analyze the sustainable management of mangroves in mitigating and adapting to climate change. This research uses a descriptive qualitative approach. The results showed that most of the community stated that the condition of the mangroves on the coast of Rembang Regency was in good condition, but in general the community had not been fully involved in Mangrove management. In mangrove ecosystems management, active community involvement is needed to maintain its sustainability. Good mangrove conditions can mitigate climate change. To adapt climate change can be done with programs that can maintain mangrove ecosystems such as using environmentally friendly fishing gear, not cutting down mangroves, developing ecotourism, and maintaining local wisdom
Acute Toxicity of domestic landfill leachate to carp fish (Cyprinus carpio)
Authors: Pratiwi H C , Rahardja B S , Tjahjaningsih W
Published: July 2022

Leachate has the potential to pollute rivers because it contains high organic and inorganic materials. Acute toxicity to fish may occur in the short term. This study aims to determine the effect of acute leachate toxicity on mortality and histopathological features of carp gill organs. This study used the Completely Randomized Design (CRD) used five leachate treatments (0.5%; 1%; 1.5%; 2% and 2.5%) with four replications. Each treatment used ten fish. The results showed that the higher the leachate concentration, the higher the mortality of carp. Even mortality reached 100% occurred in groups of fish exposed to 2.5% leachate for 96 hours. Histopathological changes in carp gill organs showed hyperplasia, hypertrophy, edema, and congestion due to exposure to leachate.
Quality improvement formulation of salak chili paste paste based on curly red chili (Capsicum annuum var. hailux) and pondoh salak (Salacca edulis reinw var. semeru) using sensory evaluation, activity evaluation of bioactive compounds and microbiological evaluation
Authors: Kusnadi Joni , Saputra Arinta Julia Buwana , Wulan Siti Narsito , Arumingtyas Estri Laras
Published: July 2022

Red Curly Chili (Capsicum annum) and Salak Pondoh (Salacca edulis Reinw) are one of Indonesia’s agricultural commodities that have high economic value. This research aims to determine the effect of salak chili paste paste formulation on its sensory properties, the effects of salak chili paste paste formulation on the levels of bioactive compounds (capsaicin, flavonoids, phenols, and antioxidant activity, and the effect of salak chili paste paste formulation on total microbes during storage. This research was divided into three stages, which is (1) sensory analysis by trained panelists, (2) analysis of bioactive compounds using a Microplate Reader, (3) analysis of the number of microbes (TPC). The results showed that (1) formulation 37.5 % Curly Red Chili var. Hailux and 12.5 % Salak Pondoh var. Semeru was preffered by the panelists, and had the highest levels of capsaicin bioactive compounds (0.539 mg/g), phenol (0.361 mg GAE/g), and flavonoids (0.462 mg QE/g). (2) formulation 12.5 % Red Chili Curly var. Hailux, and 50 % Salak Pondoh var. Semeru has the highest antioxidant activity (99.87%). (3) formulation of 50 % Salak Pondoh var. Semeru has the antimicrobial activity of 1.4 x 106 CFU/ ml.
Broodstock rearing techniques brown-marbled grouper (epinephelus fuscoguttatus) at balai perikanan budidaya air payau (BPBAP) situbondo, east java
Authors: Septian Dimas Khoironi Nur , Budi Darmawan Setia , Suciyono  , Lutfiyah Lailatul
Published: July 2022

Grouper fish has a fast growing nature so it can be mass produced to serve market demand. The purpose of implementing field work practices is to determine the technique of raising brown-marbled grouper broodstock at the Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo, East Java and to find out the problems that are often faced in the maintenance techniques of brown-marbled grouper broodstock. This research was carried out on 23 December 2019-25 January 2020. The method used is the descriptive method with data collection including primary data and secondary data. The maintenance technique for brown-marbled grouper broodstock includes several stages of activities, namely preparation of containers, procurement of broodstock and parent selection, water quality management, feeding, pest and disease control. The obstacles that exist in the maintenance technique for brown-marbled grouper broodstock are erratic weather and power outages.
The effect of barnacles powder as a source of protein feed substitution against the digestibility of crude protein and crude fiber on male rabbits rex
Authors: Agustono B , Maulana F , Warsito S H , Al-Arif M A
Published: July 2022

The aim of this study was to determine the effect of barnacle powder as a feed substitution in diet on digestibility of crude protein and crude fiber in 100 rex male rabbits. It was an experimental study using a completely randomized design with four treatments and five replications. The substitution of barnacle flour was given for four weeks and each diet was 0%, 2.5%, 5%, 7.5%. Data collection was carried out in the fourth week (for seven days) which included data on consumption, manure weight and analysis of treated feed and manure analysis. The data were analyzed using analysis of variance and Duncan test. The average crude protein digestibility (%) of P0, P1, P2, and P3 for each treatment were 76.84±0.81; 76.45±1.41; 76.09±0.60; and 77.47±1.01. The results showed that there were no significant differences (p> 0.05) in crude protein digestibility but the results showed a significant difference (p> 0.05) in crude fiber digestibility. The average crude fiber digestibility (%) of P0, P1, P2, and P3 for each treatment were 36.67±2.86; 50.81±1.57; 49.42±1.30; and 45.93±2.77. It showed that substitution of barnacle flour in diet can replace the source of protein in fish meal for the complete diet of rabbits.
Identification of Escherichia coli and Salmonella on Fishery Product from Juanda Airport, East Java, Indonesia
Authors: Hidayah R , Ulkhaq M F , Wilis R
Published: July 2022

Fishery products are foodstuffs that are prone to pathogenic microbial contamination which can cause food poisoning and disease in consumers. Therefore, fishery products need attention related to food safety. Pathogenic bacteria that often contaminate fishery products include E. coli and Salmonella which can cause disease in humans. This study aims to examine E. coli and Salmonella bacteria that contaminate fishery products that will be exported from Juanda Airport, East Java. The research method used was microscopic examination using conventional methods consisting of isolation and biochemical tests (for E. coli bacteria); prediction test, affirmation test and IMVIC test (for Salmonella bacteria). The results showed that fishery products consisting of Barramundi Skin On, Frozen Vannamei, Frozen Black Tiger Shrimp, Breaded Shrimp, Classic Frog Thighs, Skip Jack, Frozen Octopus, Frozen Mackarel, Frozen Sardine, and Frozen Squid were contaminated by bacteria that are not E. coli and Salmonella groups.
Evaluation of the culture of Spirulina sp. with Walne nutrient plus vitamin B12, KCl, NPK, ZA CaO and urea
Authors: Suyoso A L A , Sari L A , Sari P D W , Nindarwi D D , Arsad dan S
Published: July 2022

Spirulina sp. is a blue green microalga (Cyanophyceae) with cells that colonize to form spiral-shaped filaments. Spirulina has active ingredients that can function as antioxidants. Spirulina can be processed into products that have economic value and various benefits. The aim of this study was to investigate the evaluation of the culture of Spirulina sp. both on the laboratory scale and semi-mass scale, using Walne nutrient plus vitamin B12, KCl, NPK, ZA, CaO and Urea. The working method started from the preparation of tools and materials, culture media, stock of fertilizers, sterilization of tools, seed maintenance, measurements of water quality parameter and growth calculations. Culture on a laboratory scale was done with a volume of 2 L of water, while culture on a semi-mass scale was done with a volume of 80 L of water. Culture of Spirulina sp. While reached its peak of population on day 9, accounting for 9.065 cells/mL, but then the figure decreased on the following days, while on a semi-mass scale the growth continued to show an increase until day 15 with a density of 23.275 cells/mL and had not experienced any signs of decline in population.
Potential feed substitute of Cirripedia sp. flour on body weight gain, feed conversion ratio, feed consumption of buck
Authors: Agustono B , Agustin D A , Sabdoningrum E K , Yunita M N , Dewi R T , Ivani I R T
Published: July 2022

The aim of this study was to determine the effect of Cirripedia sp. flour as a fish flour substitution on body weight gain, feed conversion ratio and feed consumption of the rabbit. The experimental design used was 4 treatments and 5 replication each. This research were 100 Rex buck aged 4 months. The feed treatments used were P0 (complete feed 100% with 15% fish flour), P1(complete feed 100% with 12.5% fish flour and 2.5% Cirripedia sp. flour), P2 (complete feed 100% with 10% fish flour and 5% Cirripedia sp. flour), P3 (complete feed 100% with 7.5% fish flour and 7.5% Cirripedia sp. flour). The data were recorded at the fourth week after observation based on feed consumption, body weight gain, and feed conversion ratio. Data were analyzed with ANOVA (Analysis of Variance). Average feed consumption of P0 to P3 were 114,54, 112,09, 114,04, 112,71 g. Average of body weight gain of P0 to P3 were 20,01, 20,87, 20,72 and 20,75g. Average of feed conversion ratio of P0 to P3 were 5,79, 5,41, 5,62 and 5,48. The result showed with no significant difference (p>0.05) among the treatment in feed consumption, body weight gain, and feed conversion ratio in the rabbit.
Analysis of polycyclic aromatic hydrocarbons (PAHs) bioremediation by hydrocarbonoclastic degrading bacteria (Gordonia terrae)
Authors: Amin A. A. , Wahyuni A. R. T. , Ekawati A.W , Kurniawan A.
Published: July 2022

One of the main focuses in developing bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) is to optimize the hydrocarbonoclastic degrading bacteria such as Gordonia terrae. However, the analysis of the bacteria's capability to degrade PAHs in different concentrations is sparsely explored. This study aims to evaluate the remediation of PAHs by Gordonia terrae by analyzing bacterial activity, PAH degradation, pH, BOD, and COD. The initial PAH concentrations used were 15 ppm, 30 ppm, and 45 ppm for 14 days of incubation. The results show bacterial activity gradually increases in each concentration up to 10-day incubation and decreases in up to 14-day incubation. Moreover, the total PAHs were gradually decreased to 54%, 69%, and 77% in the 15 ppm, 30 ppm, and 45 ppm of initial concentrations, respectively. The final pH values were 6 for all concentrations. At the same time, the BOD and COD values of each concentration gradually decreased until the end of the experiments. This study shows that Gordonia terrae can degrade PAHs, which was achieved optimally after 10 days of incubation. Furthermore, this study indicates that PAH degradation is influenced by bacterial activity, pH, BOD, and COD.
Utilization of mangrove fruit (Rhizophora stylosa) as an alternative to low caffeinated drinks
Authors: Marctyas S A , Pujiastuti D Y , Saputra E
Published: July 2022

A drink that is synonymous with high caffeine content, namely coffee. One way to produce low-caffeine drinks is to make drinks from mangroves (Rhizophora stylosa). The content of compounds in mangrove fruit (Rhizophora stylosa) can be used as processed materials that can be consumed and provide benefits to human health. The purpose of this study was to determine the effect of roasting time on caffeine content. This research was experimental using a Completely Randomized Design (CRD). The treatments used were variations of roasting time, namely P0 (0 minutes), P1 (45 minutes), P2 (50 minutes) and P3 (55 minutes). The results of roasting time has a significant effect on each parameter tested. The treatment at the roasting time of 55 minutes (P3) had the best value on the parameters of colour, flavour, texture, moisture content and caffeine content. In this study, it was found that all treatments of roasting time from P0, P1, P2 and P3 had caffeine content values far below 2%, according to SNI 01-3542-2004 with a maximum of 2% and a minimum of 0,9%. The best content value was at P3 (treatment with roasting time 55 minutes) of 0,814%, which is the best result of low-caffeine mangrove drinks.
Sublethal Toxicity Insecticide Organochlorine Endosulfan on The Value of Blood Sugar Levels and The Level of Life in Tilapia (Oreochromis niloticus) Seeds
Authors: Nurhadi S , Mashitah E D , Sari dan L A
Published: July 2022

Abstrak

The use of pesticides in agriculture continues to increase. One of the classifications of pesticides is organochlorine endosulfan insecticide that can cause river pollution. One type of fish that is often cultivated in ponds is tilapia. River pollution can trigger stress and even fish death. The stress level of the fish can be seen from the value of its blood sugar levels. This research was conducted on March 20 - April 28, 2021, in Microbiology Laboratory and Anatomy-Aquaculture Laboratory, Faculty of Fisheries and Marine, Universitas Airlangga, to determine the toxicity effect of sublethal endosulfan organochlorine insecticide on blood sugar level value and tilapia seed life rate. This study used experimental methods with CRD through exposure to different organochlorine concentrations, namely P0 (0%), P1 (10%), P2 (30%), P3 (50%), P4 (70%) with four replays. Analysis of the data was processed using ANOVA and DMRT, which showed that sublethal toxicity of endosulfan organochlorine insecticides had an effect (p<0.05) on blood sugar levels and the level of tilapia seed resistance. Using only a concentration of 10% has increased blood sugar levels significantly on the 7th day and continues to increase until the 28th day (114.50 mg/dL). Also, the level of fish life insecurity indicates a value below the Indonesian National Standard (72.50 %).
Chitosan from snapper fish scale waste (Lutjanus spp.) for edible coating
Authors: Florencia A , Putra S E D , Mukti Y P
Published: July 2022

Indonesia is known for its rich fisheries sector. The portion of fish that can be consumed is only 40-50% of the total, the rest is disposed of as waste. Chitosan can be found in fish scales. This study aims to determine the effect of chitosan edible coating from scale waste in extending the shelf life of grapes and the level of consumer acceptance of chitosan edible coating. Chitosan was extracted through deproteination, demineralization, and deacetylation process. Chitosan solutions were made with various concentrations. Based on the results, the chitosan obtained had a yield of 36,32%, water content 3,86%, and ash content 89,54%. In the antibacterial activity test, the inhibition zone formed by chitosan was smaller than 0% chitosan. In the calculation of the De Garmo test obtained the concentration of 0,5% was the best concentration of chitosan. Based on the results of the shelf life estimation test, chitosan coating treatment still cannot replace paraffin wax in extending the shelf life of grapes. In the organoleptic results between samples with coating compared to samples without coating, the results show that the provision of chitosan coating can be accepted by consumers without changing the organoleptic characteristics of the grapes.
Characterization of Tilapia collagen-loaded chitosan nanofibers synthesized by electrospinning method for wound dressing
Authors: Rani C A M , Safira A , Suryadiningrat M , Fikri F , Wardhana D K , Purnama M T E
Published: July 2022

Burns are traumatic injuries that require intensive therapy. Tilapia skin is known to contain high collagen properties and was developed for wound dressing. This study aimed to investigate the characterization of Tilapia collagen-loaded chitosan nanofibers synthesized by electrospinning for wound dressing. This study evaluated three group materials, i.e. (G1) chitosan, (G2) Tilapia collagen, and (G3) Tilapia collagen-loaded chitosan. The electrospun method was performed by alloy the chitosan nanofibers containing 8% w/v Tilapia collagen. The mix solution was characterized using scanning electron microscopy (SEM), Fourier- transform infrared (FTIR) spectroscopy, pH level, swelling test, water capacity test, and cytotoxicity test. As result, Tilapia collagen-loaded chitosan showed the better swelling test, water capacity test, cytotoxicity test, and FTIR evaluation compared to the collagen or chitosan group. However, all group materials showed similarities in pH level and SEM evaluation. In conclusion, Tilapia collagen-loaded chitosan nanofibers could be considered as a wound dressing based on positive results during characterization.
The Effect of Laserpuncture on Accelerate Gonadal Maturity of Female Striped Catfish (Pangasianodon hypophthalmus)
Authors: Patmadevi M , Mukti A T , Mubarak A S , Astuti S D
Published: July 2022

This study aimed to determine the effect of different laserpuncture doses on the gonadal maturity of female striped catfish. The fish 800 to 1000 g with I gonad maturity were used. This study was used a red diode soft-laser with doses of 0.2, 0.4, 0.6, and 0.8 Joule. The positive control was injected chorionic gonadotropin hormone (CGH) and ovaprim™ intramuscular and the negative control was not used any treatment. Red diode soft-laser was shooted at the reproductive point every week for three weeks, while the CGH and ovaprim™ were injected in the final week. Gonad maturity level and gonadosomatic index (GSI) were measured in the last week. The result showed that laserpuncture had significant effect (P<0.05) on the gonadal maturity and GSI of female striped catfish. A dose of 0.4 Joule laserpuncture have the best gonadal maturity level in stage IV. Meanwhile, highest GSI were treated at dose 0.4 Joule laserpuncture, which was 3.4%. Induction of laserpuncture at the reproductive point once a week for three weeks accelerate gonadal maturity of female striped catfish.
The Intensive Program of Hatchery Aquaculture Performance of Gourami (Osphronemus gouramy) in IPB Pandaan
Authors: Pribawastuti V B , Samara S H
Published: July 2022

Gourami is an important economic fish in Indonesia. The increasing demand for gouramy must be balanced with increased seed production by aquaculture activity. One of the important activities in the gouramy aquaculture is hatchery. This study aims to determine the performance of gouramy hatchery at IPB Pandaan. The working method used in this study was descriptive method by utilizing primary and secondary data. The hatchery gouramy in IPB showed good performance with fertilization rate with value of 72.36% and hatching rate of 85.55%. The water quality parameters were also suitable for hatchery gouramy activity. The temperature ranged from 28-29°C, pH levels ranged from 7.1-7.9, DO level ranged from 4.32-5.79 mg/L.
The effect of different maceration solutions towards characteristic gelatin from bone of common carp (Cyprinus carpio)
Authors: Ristyanti E , Triastuti J , Saputra E
Published: July 2022

Gelatin is a derivative of collagen which is found in connective tissue, skin and bones. Gelatin can be obtained from cow, pig or fish. Teleost fish bones have collagen protein content ranging from 15-17%. Common carp belong to the teleost fish group and research on gelatin from carp bones using different maceration solutions has never been done before. The purpose of this researchment was to determine the effect of different maceration solutions on the characteristics of gelatin from carp bones and to determine their conformity with the standard. This research is a laboratory experimental with Completely Randomized (CRD) consisting of 3 different designs of maceration solutions (NaOH; C6H8O7and a mixture of NaOH and C6H8O7). The results showed that there was an effect in different maceration solutions on the characteristics of carp bone gelatin which was tested with the best treatment using a mixture of NaOH and C6H8O7solutions. Some of the characteristics of gelatin in this researchment are accordance with standards including water content, ash content, viscosity, gel strength, as well as color and pH in several treatments.
The Effect of Rumen’s Cattle Waste as Alternative Nutrient Sources for Thalasiossira sp Aquaculture
Authors: Santanumurti M B , Ogara M I , Santoso L , Hudaidah S
Published: July 2022

The liquid from rumen’s cattle is expected to be used as nutrients in microalgae culture. This study aimed to examine the use of waste of rumen’s cattle as a source of nutrients with different doses in the culture ofThalasiossirasp. The research design used was a completely randomized design (CRD) with 5 treatments with 3 replications of (A) 1 ml conwey/l of seawater, (B) 1 ml of waste/l of sea water, (C) 10 ml of waste/l of seawater, (D) 1 ml of waste + 0.4 gr NaN03/l of seawater, and (E) 10 ml of waste + 0.4 gr NaN03/l of seawater. The calculated parameter were the population density ofThalasiossirasp., diatom diameter, protein content and water quality. The results showed that the waste of rumen’s cattle could be used as an alternative source of nutrients in the culture ofThalasiossirasp. and the peak of the population was reached on day 3. Measurement of protein content ranged from 9.9 to 11.8% and the diameter of the diatoms ranged from 5-10 m. The measurement of the water quality of the media was in the range of temperature 24-26oC, pH value 7 and salinity 32-34 ppt.
Inhibitory Rate of Chitosan Against Vibrio harveyi use in vitro method
Authors: Haryagita  , Kusdarwati R , Suprapto H
Published: July 2022

The intensification of the penaeid shrimp culture industry have been accompanied over the last two decades by an increased incidence of infectious pathogens. Vibrio sp. has become a major source of concern for shrimp culture because of their close association with low survival rates in hatcheries or growout ponds. The antibioctic use to aim this disease but it harmful to use continuously. Resistance of pathogen and retention are such side effect left after antibiotic used. Chitosan is a biocompatible and biodegradable natural polymer with established antimicrobial properties against specific microorganisms. This research demonstrates its antibacterial activity to inhibit growth of Vibrio harveyi comparing with oxoninic acid. Inhibition growth of Vibrio harveyi was tested using diffusion method on TSA added 2% NaCl by additional disc paper which has been soaked in chitosan suspension consentrations 0,8; 1,0; 1,2; 1,4; 1,6% (w/v), asetic acid 1%, and oxolonic acid disc (2μg), each treatment was repeated three times. In this study chitosan showed an antibacterial activity. The lowest antibacterial activity showed by the lowest concentration 0,8% (w/v) and the higest antibacterial activity showed by the highest concentration 1,6% (w/v).
Physical characteristic and antioxidant activities of liquid bath soap with substitution of β-carotene crude extract from Gracilaria sp.
Authors: Ircham M M , Mubarak A S , Saputra E
Published: July 2022

Synthetic antioxidant BHT is widely used in bath soap products as an antioxidant and prevents rancidity in bath soaps, but BHT is harmful to the skin if the addition is excessive. So we need natural antioxidants that can replace the role of BHT as an antioxidant. The purpose of this study was to determine the effect of substitution of BHT with β-carotene Gracilaria sp. on the physical properties of liquid bath soap and determine the best concentration of BHT substitution with β-carotene Gracilaria sp. added to liquid bath soap formulations. This study used a completely randomized design (CRD) consisting of 3 substitution treatments for BHT with β-carotene, namely (1g BHT : 0g β-carotene), (0,5g BHT : 0,5g β-carotene), and (0g BHT : 1g β-carotene) in 100 ml of liquid soap, with 6 repetitions each. Parameters observed were pH, foam stability, viscosity, hedonic, physical stability, and antioxidant activity. The results of this study the substitution of β-carotene Gracilaria sp. the liquid bath soap had a significant effect (p<0.05) on the pH parameter and no significant effect (p<0.05) on the foam stability test and viscosity test parameters. Based on the hedonic test value, soap with the addition of 0,5 g BHT: 0,5g β-carotene has the highest test value to produce the best quality soap.
Cultivation of Phytase-Producing Bacteria as Probiotic Candidate on Molasses and Tempe-Processing Waste
Authors: Gifari Zaid Al , Agistna Ihza , Anwar Khairil , Rosyidi Anwar , Ali Muhamad , Amin Muhamad
Published: July 2022

Microbes that produce phytase enzymes are needed for livestock and fisheries. Animal feed treated with phytase will increase the availability of minerals, amino acids, and energy. For this reason, it is essential to growing phytase-producing bacteria on alternative media that are cheap and easy to obtain. This study aims to determine the ability of some bacteria that produce phytase to grow on alternative media such as molasses and tempeh waste. The indicators used were the growth rate of several phytase-producing bacteria within 3, 4, 5, and 6 hours of culture. The results showed that some bacteria were able to grow on alternative media of tempeh and molasses waste, although slower than growth on commercial media (LB). For this reason, tempeh and molasses waste can be used as alternative media to grow phytase-producing bacteria to produce cheap probiotics for livestock and fisheries.
Reproductive ability of endemic species of Lake Ajamaru Melanotaenia ajamaruensis Allen & Cross, 1980 on spawning at various sex ratio
Authors: Said D S , Mayasari N , Kadarini dan T
Published: July 2022

Melanotaenia ajamaruensis or Ajamaru Rainbowfish is endemic species of Lake Ajamaru, Papua, Indonesia. It belongs to Rainbowfish group, with data deficient status. They have attractive color, especially on male and has potential as an ornamental fish, but the percentage of male is low. A study was conducted to find out their reproductive ability at various sex ratios (male:female = 1:1; 1:2; or 1:3) with two replications for 40 days (January-February 2021) at Research Center for Limnology, LIPI. Observations were also conducted on sexual maturity age, egg diameter, size at a certain age. The ratio of 1:3 have the highest value on number of spawning (NoS): 25 times, number of eggs (NoE)/female: 54 eggs, number of larvae (NoL)/female: 49 larvae, fertilization rate/FR (%): 97.16; hatching rate/HR (%): 97.93; larvae survival at seven days /SR7 (%): 90.34, and eggs incubation time/EIT (days): 5.24. The sexual maturity on 8 months old. Egg diameter (mm): 0.98±0.043, larvae size (mm) at (0 day), and at 1, 2, 4, 8, 12 weeks old were: 3.2±0.68; and 6±0.74; 11.70±1.77; 19.48±1.86; 21.65±2.64; 25.0±2.94 respectively. Daily growth rate 0.26 mm/day That is indicates that M ajamaruensi could adapted well to ex-situ habitat and the reproduction could takes place with a small number of males (1:3).
Length-weight relationships and condition factors of fifteen fish species from Pabean Bay, West Java, Indonesia
Authors: Syafei L S , Sudinno D , Parawangsa I N Y
Published: July 2022

Length-weight relationships (LWR) and condition factors (CF) were calculated for thirteen fish species using 621 specimens from Pabean Bay, West Java, Indonesia. This study is the first reference on length–parameters and condition factors for fifteen species, namely: Ambassis vaschelli, Arius sp., Asterhombus intermedius, Butis amboinensis, Eleuthronema tetradactylum, Gerres abbreviatus, Johnius coitor, Glosogobius sirkumspelitus, Karalla dussumieri, Lutjanus ruselli, Pomadasys kaakan, Pseudorhombus arsius, Sardinella gibbosa, Strongylura strongylura, and Terapon jarbua. Fish caught montly from January to December 2015, using gillnet and set net. The results of LWR and CF from fifteen fish species indicated that the growth pattern was: 3 species were allometric positive (b>3), 1 species was isometric (b=3), and 11 species were allometric negative (b<3).
Study on the potential of sea urchin Tripneustes gratilla (Linnaeus, 1758) as a bioindicator dangerous plastic pollution in environment of gunungkidul beach Yogyakarta
Authors: Pratita S D , Gavintri M B , Rizkyta A N , Khasanah L U , Ponkiyawati F A , Retnoaji B
Published: July 2022

Yogyakarta has a lot of beaches, especially in the Gunungkidul district. Sepanjang and Nglolang are tourist beaches in Gunungkidul, while Porok is a research station for the Faculty of Biology UGM. One common types of waste in the ocean is microplastic. The presence of microplastics in aquatic ecosystems in high numbers can threaten the population of benthic organisms and the stability of the ecosystem. Tripneustes gratilla, one of the sea urchins, can be used as an indicator of environmental pollution because it eats algae and detritus at the bottom of the water. This study aims to determine the microplastic content in seawater, beach substrates, and Tripneustes gratilla organs. Sampling by purposive random sampling, Analysis of microplastics in water, substrate, intestinal and gonads organs. The results obtained that there were four types of microplastics found: fragments, films, fiber, and monofilaments. The most common type of microplastic found is film. The abundance of microplastics on the substrate is higher than that of water. The abundance of microplastics in the intestinal organs of T. gratilla was higher than its gonads. Based on these results, T. gratilla has the potential as a bioindicator of microplastic pollution in the coastal environment of Gunungkidul, Yogyakarta.
Opportunities and its problem for the development of Tilapia aquaculture business in Banjar Regency, South Kalimantan
Authors: Nurlaili N , Hikmah H
Published: July 2022

This paper aims to provide an overview of the potential opportunities for developing tilapia aquaculture and its development problems. The research was conducted in Karang Intan District, Banjar Regency, South Kalimantan in 2021. The method used was a sensus method to 150 respondents and conducting in-depth interviews with representatives of tilapia hatchery business actors in four villages, namely Karang Intan, Padang Panjang, Pandak Daun, and Jingah Habang Ilir. The results of the study indicate that the potential opportunities for developing hatchery aquaculture businesses in Karang Intan District include opportunities for developing aquaculture in Banjar Regency are human resources related to higher education, productive age, mastery of assets and own capital, as well as the availability of adequate land area, potential for a large enough land area owned by business actors, the enthusiasm for work from business actors and the opening of the market. The problems faced include the availability of superior broodstock, the threat of drought, a pandemic disaster due to restrictions, and a flood that only occurred in January this year.
The dominance and proportions of plankton in Pacific white shrimp (Litopenaeus vannamei) ponds cultivated with the intensive system in Bulukumba Regency, South Sulawesi, Indonesia
Authors: Sani M D , Wiradana P A , Maharani A Y , Mawli R E , Mukti A T
Published: July 2022

This study aimed to evaluate plankton in Pacific white shrimp (Litopenaeus vannamei) cultivation in Bulukumba Regency, South Sulawesi. The study was conducted in six intensive ponds for 84 days from November 2019 to February 2020. Plankton samples were collected every 10 days for 8 weeks based on the day of culture (DOC) of Pacific white shrimp since the first rearing in the pond using plankton net (mesh size of 25 μm). Then, planktons were preserved to 5% formalin buffer in 250 mL of sterile plastic. Next, the plankton densities and compositions were analyzed quantitatively and qualitatively. The results showed that plankton dominance in Chlorophyta species and the presence was evenly distributed across all shrimp ponds in the field. The number was relatively stable in all shrimp DOCs and was the highest proportion as well; Chlorophyta (73 to 83%), Diatom group (7.75 to 15.63%), and blue-green algae (BGA) group (7.13 to 13.50%). Plankton can be used as a biomonitor of pollution and shrimp health in dominance and the percentage proportion of each species. Regular monitoring is highly recommended to minimize plankton growth, especially the BGA type that can harm shrimp health in the intensive system.
The effect of bromelain enzyme on pineapple core on the relationship between platelet count and hematocrit value in carp (Cyprinus carpio) infested with Argulus japonicus
Authors: Fahturohman A B , Kismiyati  , Rahmawati Y
Published: July 2022

Carp (Cyprinus carpio) is a freshwater fish commodity that has high economic value and is growing rapidly it is widely cultivated in Indonesia. Based on data from the Maritime and Fisheries, carp production in Indonesia during 2015-2019 increased by 12.09%. Bromelain enzyme gives the best results in hematology, which can affect blood coagulation by increasing the ability of serum fibrinolytic. Purpose of this study was to determine the effect of bromelain enzyme, infestation Argulus japonicus, interaction and relationship between platelet count and hematocrit value in carp. Analysis of data used in this study is Analyze of Variance, while to determine the relationship between platelet count and hematocrit value using the Spearman Correlation. Results statistical tests showed that there was no effect (p>0.05) between different dosage enzyme bromelain in pineapple core on the platelet count, but there was an effect (p<0.05) on hematocrit value. Statistical test results showed that there was no effect (p>0.05) between infestations Argulus japonicus differentiation platelet count and hematocrit value. Results statistical tests also showed that there was no interaction and relationship between platelet count and hematocrit value in carp that were infested with Argulus japonicus and given the enzyme bromelain in pineapple core.
Molecular and phylogenetic analysis of Sardinella lemuru Bleeker 1835 at fishing ground Canggu-Bali inferred D-loop mutations of mtDNA
Authors: Winata V A , Kasitowati R D , Iranawati F , Pranowo W S , Sartimbul A
Published: July 2022

Sardinella lemuru resources are abundant, nutritious, high omega-3 and have high economic value in artisanal fisheries. Its production volume landed for over a decade, but the most significant increase was peak southeast monsoon 2019 at Canggu fishing ground. However, prior-conventional kinship assessments genetically need to be confirmed. These research objectives encompass both molecular identification and genetic relationship of Canggu S. lemuru with the others. Similar morphological samples had collected of ten individuals. Chelex 10% was used for genomic DNA extraction, CRK-CRE primer with Hotstart-50 PCR thermal profile had applied for amplification. 1% agarose electrophoresis and Sanger sequencing had examined for both quantity and quality of D-loop amplicon. Identification using the algorithm of BLASTn. Phylogenetic tree analysis uses the neighbor-joining method. The genetic identity of 10 D-loop Canggu samples was high (97.46%-98.47%) as Philippines S. lemuru (MK579633.1-MK579742.1). The phylogenetic significantly shows low barrier inter-waters from low to moderate genetic distance. Inter-waters samples are very close in intraclade, but it still has a different mutation rate in another clade. Means suggested that Canggu S. lemuru is more diverse in nucleotide base substitutions encouraging high variation. These study results provide needed information that southern translocation occurred among waters, its dispersal connection.
Description of Silver Barb Barbonymus gonionotus (Bleeker, 1849) (Cypriniformes: Cyprinidae) From Madura Island, Indonesia
Authors: Valen F S , Hasan V , Ottoni F P , Nafisyah A L , Erwinda M , Annisa A N
Published: July 2022

Barbonymus gonionotus, a fish species in the family Cyprinidae, is known from Western Indonesia. In Java, it was previously known only from the mainland and had never been reported from Madura Island, east of Java This paper provides the first description of B. gonionotus from the Kemuning River in Madura Island. The specimens of B. gonionotus were characterized as follows: dorsal fin rays 12; ventral fin rays 9; pectoral-fin rays 15-16; anal-fin rays 9. Description of specific morphological characters of sampled specimens is provided.
The effects of different feeding rates on the growth of silver rasbora (Rasbora argyrotaenia)
Authors: Nisak L , Agustono  , Budi D S
Published: July 2022

This study was purposed to know the effects of feeding rate on growth and feed conversion ratio and obtain the optimal feeding rate in silver rasbora (Rasbora argyrotaenia) culture. The study was conducted on January-March 2019 in the aquaculture facility and laboratory of Universitas Airlangga, Banyuwangi Campus. A total of 400 silver rasbora fry with a total length of 2.5 ± 0.5 cm and body weight of 266.1 ± 9.51 mg was obtained from the aquaculture facility of Universitas Airlangga, Banyuwangi Campus. There are 5 feeding rate treatments (4 replicates) in this study including at satiation, 1 %, 3 %, 5 %, and 7 % from fish biomass. All treatments and replication were conducted on 20 glass aquaria (30 × 40 × 50 cm3) with a 20 fish/aquarium stocking density for 40 days. Based on the results, the feeding rate significantly (P < 0.05) effect to all parameters. The optimum feeding rate in this study is 3 %, where lower feeding consumption obtained the same growth with other treatments.
Extraction of Stingray Liver Oil (Dasyatis Sp) with Alkaline Digestion Method
Authors: Joesidawati M I
Published: July 2022

Stingray liver oil extraction method (Dasyatis sp) using alkaline digestion method with the addition of 2% concentrated NaOH reagent and HCL to maintain pH 8-9 by heating at 50°C for 3 hours can produce a yield of 35.74±0.38%. then with the addition of 3% magnesol XL adsorbent and centrifuged for 15 minutes at a speed of 5000 rpm can improve the quality of stingray liver oil (Dasyatis sp) by producing an FFA value of 0.40%±0.02, PV of 2.37±0, 05 mEq/kg, p-anisidin 5.72±1.95 mEq/kg, TOTOX 8.27±1.80 mEq/kg, AV 1.74±0.01 mg NaOH/kg, clarity 75, 19±1.7%.
The effect of fortification Moringa oleifera leaves powder on calcium content in otak-otak products of Clarias sp.
Authors: Sulistiyati T D , Suprayitno E , Djamaludin H , Tambunan J E , Muchayaroh U
Published: July 2022

Clarias sp. is one of the most common types of freshwater fish. The utilization of Clarias sp. is only limited to fresh consumption, whereas the nutritional content of Clarias sp. is potential as a source of nutrition. High protein and low-fat content make Clarias sp. can be processed into diversification of traditional food such as otak-otak products. The otak-otak products can be used as functional food, but there is a weakness i.e. the low of Calcium of otak-otak products. This study aims to determine (1) fortification of Moringa oleifera leaves on organoleptic and (2) mineral content of Calcium the otak-otak products. The method used is an experimental method with a simple Completely Randomized Design. Independent variables of this study were concentration of Moringa oleifera leaves powder 4%, 8% and 12% while dependent variable was Calcium content. The results showed that fortification of Moringa oleifera leaves powder had a significant effect on organoleptic aroma, taste, texture, appearance and Calcium content of the otak-otak products. The best fortification of Moringa oleifera leaves powder is 4% with the results of aroma of 41.00; taste 37.25; texture 38.00 and appearance of 38.05. Calcium content of otak-otak products reached 3.09±0.099 mg.
Application of Modified Starch in the Carragenan-Based Biodegradable Packaging from Eucheuma cottonii on Biodegradablility and Mechanical Properties
Authors: Islamiyah H S , Alamsjah M A , Pujiastuti D Y
Published: July 2022

The massive plastic production in Indonesia is an effort to meet the demand for the food packaging sector, which is nearly 40% of the total plastic demand. Plastics are not environmentally friendly because they are not degradable. Carrageenan-based biodegradable packaging in the form of bioplastic is an alternative, but it has disadvantages such as low resistance to water. Modified starch can be used to improve bioplastic characteristics. This research aimed to determine the application of modified starch in biodegradable packaging to its biodegradability and mechanical properties. This research is experimental which consists of primary parameters and supporting parameters. The independent variables used were modified starch (0, 1, 1.5, and 2 g). The experimental design used a completely randomized design (CRD) and data analysis using the Analysis of Variance (ANOVA) and the DMRT follow-up test. The results showed that the application of modified starch in biodegradable packaging based on carrageenan had a significant effect (p <0.05) on water resistance and the best test results were 2 g of modified starch (P3) with a water resistance value of 50.96% ± 4.86. Meanwhile, the results of biodegradability, tensile strength, and elongation were not significantly different.
First country record of the bearded gudgeon Pogoneleotris heterolepis (Günther, 1869) (Teleostei: Eleotridae) from Indonesia
Authors: Valen Fitri Sil , Hasan Veryl , Ottoni Felipe P. , Nafisyah Ayu Lana , Erwinda Mugi , Annisa Ana Nur , Adis M A
Published: July 2022

A bearded gudgeon Pogoneleotris heterolepis (Günther, 1869) with c. 19 cm of total length was photographed on 10 June 2021 in Kapuas river, West Kalimantan province, Indonesia. This rare finding is represent a first country record of P. heterolepis in Indonesia after previously only recorded in Sarawak, Malaysia.
The Effect of Turmeric (Curcuma domestica) Addition ont The Consumer Acceptance Level of Milkfish Crackers
Authors: Aulia Nabila , Andriyono Sapto , Saputra Eka
Published: July 2022

Milkfish (Chanos chanos) crackers are Indonesian snacks made from fish, which are quite popular among communities. Indonesia has many spices that can be used as food additives, such as turmeric for natural coloring. Turmeric (Curcuma domestica) has an aromatic odor and contains curcuminoids which are beneficial for the body. This study was aimed to determine the consumer acceptance of milkfish crackers added with different amount of turmeric concentration. This research was conducted by using Completely Randomized Design (CRD) with one factor which is the different amount of turmeric added consisting of 4 concentration with 3 replications. The results showed that there was a significant difference between treatments (P<0.05), the results of nutritional value showed the best treatment for milkfish crackers with the addition 0.05 g turmeric with an average value of water content (2.64%), ash content (1.75%), protein content (10.54%), fat content (7.51%), and carbohydrate content (77.53%). The result of the organoleptic test showed the best treatment for the milkfish crackers with the addition 0.5 g of turmeric with an average value of color (8.6), aroma (8.4), taste (8.53), and texture (8.8).