bluuebunny/crossref_metadata_2025 huggingface dataset:
Latest Performance Improvement Strategies and Techniques Used in 5G Antenna Designing Technology, a Comprehensive Study
Authors: Ahmad Iftikhar , Tan Wenhao , Ali Qasim , Sun Houjun
Published: April 2022

In the recent era, fifth-generation technology (5G) has not been fully implemented in the realm of wireless communication. To have excellent accessible bandwidth feasibility, and in order to achieve the aims of 5G standards, such as higher data rates and ultrahigh-definition video streaming, the millimeter wave (mmWave) band must be employed. Services with minimal latency and many other features are feasible only in the mmWave spectrum. To avoid numerous communication complexities such as high connection losses, short wavelength, and restricted bandwidth, as well as path-loss challenges in the mmWave range, an antenna with wide bandwidth, high gain, narrow steerable beam, high isolation, low side-lobe levels, and multiband features is required to alleviate these difficulties and meet 5G communication standards. To overcome these challenges, specific strategies and techniques should be employed in the traditional antenna designing procedure to excellently improve the performance of the antenna in terms of bandwidth, gain, and efficiency and to reduce the mutual coupling effect between the closely colocated antenna elements in MIMOs and arrays. The researchers reported on a variety of bandwidth and gain improvement approaches. To gain broader coverage, traditional antenna design techniques must be modified. In this study, the latest state-of-the-art work is reviewed, such as the role of the metamaterials (MMTs), parasitic patches, hybrid feeding, EBG structure, impact of the slots with different geometrical shapes in the radiator to achieve the goal of wide bandwidth, boosted gain, reduced side-lobes level, as well as stable radiation properties. Mutual coupling reduction techniques are also briefly reported. The role of reconfigurability is focused on in this study, and at the end, the future challenges in the field of antenna design and possible remedies to such issues are reviewed.
Endosymbiotic Bacterial Diversity of Corn Leaf Aphid, Rhopalosiphum maidis Fitch (Hemiptera: Aphididae) Associated with Maize Management Systems
Authors: Csorba Artúr Botond , Fora Ciprian George , Bálint János , Felföldi Tamás , Szabó Attila , Máthé István , Loxdale Hugh D. , Kentelky Endre , Nyárádi Imre-István , Balog Adalbert
Published: April 2022

In this study, different maize fields cultivated under different management systems were sampled to test corn leaf aphid, Rhopalosiphum maidis, populations in terms of total and endosymbiotic bacterial diversity. Corn leaf aphid natural populations were collected from traditionally managed maize fields grown under high agricultural and natural landscape diversity as well as conventionally treated high-input agricultural fields grown in monoculture and with fertilizers use, hence with low natural landscape diversity. Total bacterial community assessment by DNA sequencing was performed using the Illumina MiSeq platform. In total, 365 bacterial genera were identified and 6 endosymbiont taxa. A high abundance of the primary endosymbiont Buchnera and secondary symbionts Serratia and Wolbachia were detected in all maize crops. Their frequency was found to be correlated with the maize management system used, probably with fertilizer input. Three other facultative endosymbionts (“Candidatus Hamiltonella”, an uncultured Rickettsiales genus, and Spiroplasma) were also recorded at different frequencies under the two management regimes. Principal components analyses revealed that the relative contribution of the obligate and dominant symbiont Buchnera to the aphid endosymbiotic bacterial community was 72%, whereas for the managed system this was only 16.3%. When facultative symbionts alone were considered, the effect of management system revealed a DNA diversity of 23.3%.
Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective
Authors: Silva Vanessa , Correia Susana , Rocha Jaqueline , Manaia Célia , Silva Adriana , García-Díez Juan , Pereira José , Semedo-Lemsaddek Teresa , Igrejas Gilberto , Poeta Patrícia
Published: April 2022

Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.
Impact of Grain-Coating Clays on Porosity Preservation in Paleocene Turbidite Channel Sandstones: Nelson Oil Field, UK Central North Sea
Authors: Bello Abdulwahab Muhammad , Jones Stuart J. , Gluyas Jon , Al-Ramadan Khalid
Published: April 2022

The Forties Sandstone Member is an important deep-water reservoir in the Central North Sea. The role of depositional characteristics, grain-coating clays, and diagenesis in controlling the reservoir quality of the sandstones is poorly understood. The main aim of the study is to understand the role of depositional characteristics, grain-coating and pore-filling clays, and diagenesis in controlling the reservoir quality evolution of turbidite-channel sandstones. The study employed a multi-disciplinary technique involving thin section petrography and scanning electron microscopy (SEM) to investigate the impact of grain size, clay matrix content, mode of occurrence of grain-coating chlorite and illite, and their impact in arresting quartz cementation and overall reservoir quality in the sandstones. Results of our study reveal that porosity evolution in the sandstones has been influenced by both primary depositional characteristics and diagenesis. Sandstones with coarser grain size and lower pore-filling clay content have the best reservoir porosity (up to 28%) compared to those with finer grain size and higher pore-filling clay content. Quartz cement volume decreases with increasing clay-coating coverage. Clay coating coverage of >40% is effective in arresting quartz cementation. Total clay volume of as low as 10% could have a deleterious impact on reservoir quality. The Forties Sandstone Member could potentially be a suitable candidate for physical and mineralogical storage of CO2. However, because of its high proportion (>20%) of chemically unstable minerals (feldspar, carbonates, and clays), their dissolution due to CO2 injection and storage could potentially increase reservoir permeability by an order of magnitude, thereby affecting the geomechanical and tensile strength of the sandstones. Therefore, an experimental study investigating the amount of CO2 to be injected (and at what pressure) is required to maintain and preserve borehole integrity. The findings of our study can be applied in other reservoirs with similar depositional environments to improve their reservoir quality prediction.
Synthesis of Iron Nanomaterials for Environmental Applications from Hydrometallurgical Liquors
Authors: Mystrioti Christiana , Papassiopi Nymphodora , Xenidis Anthimos
Published: April 2022

Hydrometallurgical leaching solutions are often rich in iron, which was traditionally considered a major impurity. However, iron can be selectively separated and recovered by applying appropriate solvent extraction and stripping techniques, and the resulting solutions can be valorized for the synthesis of high-added-value products, such as magnetic iron oxide nanoparticles (mIONPs). The aim of this study was to synthesize high-quality mIONPs from solutions simulating the composition of two alternative stripping processes. The precursor solutions consisted of Fe(II) in an acidic sulfate environment and Fe(III) in an acidic chloride medium. The Fe(II)-SO4 solution was treated with a mixture of KNO3-KOH reagents, and the product (M(II)) was identified as pure magnetite with a high specific magnetization of 95 emu·g−1. The treatment of Fe(III)-Cl solution involved the partial reduction of Fe(III) using metallic iron and the co-precipitation of iron cations with base addition combined with microwave-assisted heating. The product (M(III)) was a powder, which consisted of two phases, e.g., maghemite (75%) and magnetite (25%), and also had a high magnetic saturation of 80 emu·g−1. The nanopowders were evaluated for their effectiveness in removing Cr(VI) from contaminated waters. The maximum adsorption capacity was found to be equal to 11.4 and 17.4 mg/g for M(II) and M(III), respectively. The magnetic nanopowders could be easily separated from treated waters, a property that makes them promising materials for the water treatment sector.
In Situ FIB-TEM-TOF-SIMS Combination Technique: Application in the Analysis of Ultra-Light and Trace Elements in Phyllosilicates
Authors: Tai Kairui , Li Yang , Liu Shen , Guo Zhuang , Li Chen , Du Lin , Zhao Sizhe , Li Xiongyao , Liu Jianzhong
Published: April 2022

At present, a single technical method has difficulty in obtaining microscopic data of ultra-light elements, trace elements, and crystal structures in samples simultaneously. This work combined an in situ focused ion beam—transmission electron microscopy—time of flight secondary ion mass spectrometry (FTT) technique and analyzed the composition and crystal structure of four phyllosilicate samples. These materials were comprised of antigorite, clinochlore, and cookeite phases. An FIB sample preparation technique was found to provide a sample thickness suitable for TEM observations and a degree of surface roughness appropriate for TOF-SIMS analysis. In addition, the relative amounts and distributions of various elements could be obtained, as well as crystal structure data, such that the composition and crystal structure of each specimen were determined. The in situ FTT method demonstrated herein successfully combines the advantages of all three analytical techniques and offers unique advantages with regard to analyzing ultra-light and trace elements as well as the structural data of phyllosilicates.
Geological, Mineralogical and Geochemical Study of the Aquamarine-Bearing Yamrang Pegmatite, Eastern Nepal with Implications for Exploration Targeting
Authors: Bhandari Sushmita , Qin Kezhang , Zhou Qifeng , Evans Noreen J.
Published: April 2022

The Yamrang Pegmatite in the Ikhabu Pegmatite Field, Eastern Nepal is Nepal’s primary source of aquamarine. This paper reports detailed mineralogy and whole rock granite and pegmatite geochemistry, and major and trace element data for alkali feldspar and muscovite in order to classify the aquamarine-bearing Yamrang Pegmatite, elucidate beryl-saturation processes and evaluate potential geochemical exploration tools for beryl-pegmatites. Five internal mineralogical/textural zones were identified in the Yamrang Pegmatite; zone 1 (saccharoidal albite); zone 2 (blocky perthitic microcline); zone 3 (muscovite–microcline–quartz); zone 4 (beryl-quartz), and zone 5 (miarolitic cavities). Zones 1–4 represent the magmatic stage, while zone 5 formed during the hydrothermal stage of pegmatite genesis. Spectacular aquamarines are recovered from miarolitic zone 5, while beryl saturation is found in zones 3, 4, and 5. Based on beryllium (Be) content, Be partition among co-existing minerals at the magmatic stage is beryl > muscovite > tourmaline > alkali feldspar > quartz. In contrast, the sequence at the hydrothermal stage is beryl > muscovite > albite > tourmaline > quartz. The Be content in rock-forming minerals decreases from pegmatite margin to core, and tourmaline could have played a significant role in Be enrichment processes in the marginal pegmatite zone. High temperature, a low degree of fractionation, and the dominance of Be-compatible mineral phases such as muscovite, calcium-rich alkali feldspar and tourmaline resulted in beryl undersaturation in marginal zones. However, low temperature, high fractional crystallization, and low abundance of Be-compatible mineral phases resulted in beryl saturation in inner zones. The strongly peraluminous nature, low total REE content (<500 ppm), mineral assemblage of beryl, tourmaline, spessartine, columbite-tantalite, depletion of Ba, Nb, and enrichment of Pb, Rb, Cs in the primitive mantle normalized multi-element plots suggest that the beryl-bearing Yamrang Pegmatite corresponds to the LCT pegmatite family. Alkali feldspar with K/Rb values of 30–150, Rb ~3000 ppm, Cs >100 ppm, and muscovite, with K/Rb ranging 18–50, Rb ~6000 ppm, Cs > 500 ppm, and Ta > 65 ppm in inner zones (3–5), indicate that the Yamrang Pegmatite is an intermediate-fractionated, beryl-type rare-element (REL) pegmatite. It is probable that whole rock Be content of >10 ppm could be considered an exploration guide to beryl mineralization in the region.
Dioscin-Mediated Autophagy Alleviates MPP+-Induced Neuronal Degeneration: An In Vitro Parkinson’s Disease Model
Authors: Azam Shofiul , Haque Md. Ezazul , Cho Duk-Yeon , Kim Joon-Soo , Jakaria Md. , Kim In-Su , Choi Dong-Kug
Published: April 2022

Autophagy is a cellular homeostatic process by which cells degrade and recycle their malfunctioned contents, and impairment in this process could lead to Parkinson’s disease (PD) pathogenesis. Dioscin, a steroidal saponin, has induced autophagy in several cell lines and animal models. The role of dioscin-mediated autophagy in PD remains to be investigated. Therefore, this study aims to investigate the hypothesis that dioscin-regulated autophagy and autophagy-related (ATG) proteins could protect neuronal cells in PD via reducing apoptosis and enhancing neurogenesis. In this study, the 1-methyl-4-phenylpyridinium ion (MPP+) was used to induce neurotoxicity and impair autophagic flux in a human neuroblastoma cell line (SH-SY5Y). The result showed that dioscin pre-treatment counters MPP+-mediated autophagic flux impairment and alleviates MPP+-induced apoptosis by downregulating activated caspase-3 and BCL2 associated X, apoptosis regulator (Bax) expression while increasing B-cell lymphoma 2 (Bcl-2) expression. In addition, dioscin pre-treatment was found to increase neurotrophic factors and tyrosine hydroxylase expression, suggesting that dioscin could ameliorate MPP+-induced degeneration in dopaminergic neurons and benefit the PD model. To conclude, we showed dioscin’s neuroprotective activity in neuronal SH-SY5Y cells might be partly related to its autophagy induction and suppression of the mitochondrial apoptosis pathway.
Chemical Diversity and Potential Target Network of Woody Peony Flower Essential Oil from Eleven Representative Cultivars (Paeonia × suffruticosa Andr.)
Authors: Lei Gaoming , Song Chaoying , Wen Xinyue , Gao Guoyu , Qi Yanjie
Published: April 2022

Woody peony (Paeonia × suffruticosa Andr.) has many cultivars with genetic variances. The flower essential oil is valued in cosmetics and fragrances. This study was to investigate the chemical diversity of essential oils of eleven representative cultivars and their potential target network. Hydro-distillation afforded yields of 0.11–0.25%. Essential oils were analyzed by GC-MS and GC-FID which identified 105 compounds. Three clusters emerged from multivariate analysis, representative of phloroglucinol trimethyl ether (‘Caihui’), citronellol (‘Jingyu’, ‘Zhaofen’ and ‘Baiyuan Zhenghui’) and mixed (the rest of the cultivars) chemotypes. ‘Zhaofen’ and ‘Jingyu’ also exhibited low levels of other rose-related compounds. The main components were subjected to a target network approach. Drug-likeness screening gave 20 compounds with predictive blood–brain barrier permeation. Compound target network identified six key compounds, namely nerol, citronellol, geraniol, geranic acid, cis-3-hexen-1-ol and 1-hexanol. Top enriched terms in GO, KEGG and DisGeNET were mostly related to the central nervous system (CNS). Protein—protein interactions revealed a core network of 14 targets, 11 of which were CNS-related (targets for antidepressants, analgesics, antipsychotics, anti-Alzheimer’s and anti-Parkinson’s agents). This work provides useful information on the production of woody peony essential oils with specific chemotypes and reveals their potential importance in aromatherapy for alternative treatment of CNS disorders.
Interactions among Endothelial Nitric Oxide Synthase, Cardiovascular System, and Nociception during Physiological and Pathophysiological States
Authors: Sarmah Niribili , Nauli Andromeda M. , Ally Ahmmed , Nauli Surya M.
Published: April 2022

Nitric oxide synthase (NOS) plays important roles within the cardiovascular system in physiological states as well as in pathophysiologic and specific cardiovascular (CV) disease states, such as hypertension (HTN), arteriosclerosis, and cerebrovascular accidents. This review discusses the roles of the endothelial NOS (eNOS) and its effect on cardiovascular responses that are induced by nociceptive stimuli. The roles of eNOS enzyme in modulating CV functions while experiencing pain will be discussed. Nociception, otherwise known as the subjective experience of pain through sensory receptors, termed “nociceptors”, can be stimulated by various external or internal stimuli. In turn, events of various cascade pathways implicating eNOS contribute to a plethora of pathophysiological responses to the noxious pain stimuli. Nociception pathways involve various regions of the brain and spinal cord, including the dorsolateral periaqueductal gray matter (PAG), rostral ventrolateral medulla (RVLM), caudal ventrolateral medulla, and intermediolateral column of the spinal cord. These pathways can interrelate in nociceptive responses to pain stimuli. The alterations in CV responses that affect GABAergic and glutamatergic pathways will be discussed in relation to mechanical and thermal (heat and cold) stimuli. Overall, this paper will discuss the aggregate recent and past data regarding pain pathways and the CV system.
Semi-Synthesis of N-Aryl Amide Analogs of Piperine from Piper nigrum and Evaluation of Their Antitrypanosomal, Antimalarial, and Anti-SARS-CoV-2 Main Protease Activities
Authors: Wansri Rattanaporn , Lin Aye Chan Khine , Pengon Jutharat , Kamchonwongpaisan Sumalee , Srimongkolpithak Nitipol , Rattanajak Roonglawan , Wilasluck Patcharin , Deetanya Peerapon , Wangkanont Kittikhun , Hengphasatporn Kowit , Shigeta Yasuteru , Liangsakul Jatupol , Suroengrit Aphinya , Boonyasuppayakorn Siwaporn , Chuanasa Taksina , De-eknamkul Wanchai , Hannongbua Supot , Rungrotmongkol Thanyada , Chamni Supakarn
Published: April 2022

Piper nigrum, or black pepper, produces piperine, an alkaloid that has diverse pharmacological activities. In this study, N-aryl amide piperine analogs were prepared by semi-synthesis involving the saponification of piperine (1) to yield piperic acid (2) followed by esterification to obtain compounds 3, 4, and 5. The compounds were examined for their antitrypanosomal, antimalarial, and anti-SARS-CoV-2 main protease activities. The new 2,5-dimethoxy-substituted phenyl piperamide 5 exhibited the most robust biological activities with no cytotoxicity against mammalian cell lines, Vero and Vero E6, as compared to the other compounds in this series. Its half-maximal inhibitory concentration (IC50) for antitrypanosomal activity against Trypanosoma brucei rhodesiense was 15.46 ± 3.09 μM, and its antimalarial activity against the 3D7 strain of Plasmodium falciparum was 24.55 ± 1.91 μM, which were fourfold and fivefold more potent, respectively, than the activities of piperine. Interestingly, compound 5 inhibited the activity of 3C-like main protease (3CLPro) toward anti-SARS-CoV-2 activity at the IC50 of 106.9 ± 1.2 μM, which was threefold more potent than the activity of rutin. Docking and molecular dynamic simulation indicated that the potential binding of 5 in the 3CLpro active site had the improved binding interaction and stability. Therefore, new aryl amide analogs of piperine 5 should be investigated further as a promising anti-infective agent against human African trypanosomiasis, malaria, and COVID-19.
Green Synthesized ZnO Nanoparticles as Biodiesel Blends and Their Effect on the Performance and Emission of Greenhouse Gases
Authors: Kavalli Kiran , Hebbar Gurumoorthy S. , Shubha Jayachamarajapura Pranesh , Adil Syed Farooq , Khan Mujeeb , Hatshan Mohammad Rafe , Almutairi Adibah Mukhlid , Shaik Baji
Published: April 2022

Pollution and global warming are a few of the many reasons for environmental problems, due to industrial wastes and greenhouse gases, hence there are efforts to bring down such emissions to reduce pollution and combat global warming. In the present study, zinc oxide nanoparticles are green synthesized using cow dung as fuel, through combustion. Synthesized material was characterized by FTIR, XRD, UV, and FESEM. The as-prepared ZnO-GS NPs were employed as a transesterification catalyst for the preparation of biodiesel from discarded cooking oil. The biodiesel obtained is termed D-COME (discarded cooking oil methyl ester), which is blended with 20% commercial diesel (B20). Additionally, this blend, i.e., B20, is further blended with varying amounts of as-prepared ZnO-GS NPs, in order to ascertain its effects on the quality of emissions of various greenhouse gases such as hydrocarbons, COx, NOx. Moreover, the brake thermal efficiency (BTHE) and brake specific fuel consumption (BSFC) were studied for their blends. The blend (B20) with 30 mg of ZnO-GS, i.e., B20-30, displays the best performance and reduced emissions. Comparative studies revealed that the ZnO-GS NPs are as efficient as the ZnO-C NPs, indicating that the green synthetic approach employed does not affect the efficiency of the ZnO NPs.
Dynamic Phenomena and Complexation Effects in the α-Lithiation and Asymmetric Functionalization of Azetidines
Authors: Musci Pantaleo , Colella Marco , Altomare Angela , Romanazzi Giuseppe , Sheikh Nadeem S. , Degennaro Leonardo , Luisi Renzo
Published: April 2022

In this work it is demonstrated that enantiomerically enriched N-alkyl 2-oxazolinylazetidines undergo exclusive α-lithiation, and that the resulting lithiated intermediate is chemically stable but configurationally labile under the given experimental conditions that afford enantioenriched N-alkyl-2,2-disubstituted azetidines. Although this study reveals the configurational instability of the diastereomeric lithiated azetidines, it points out an interesting stereoconvergence of such lithiated intermediates towards the thermodynamically stable species, making the overall process highly stereoselective (er > 95:5, dr > 85:15) after trapping with electrophiles. This peculiar behavior has been rationalized by considering the dynamics at the azetidine nitrogen atom, the inversion at the C-Li center supported by in situ FT-IR experiments, and DFT calculations that suggested the presence of η3-coordinated species for diastereomeric lithiated azetidines. The described situation contrasted with the demonstrated stability of the smaller lithiated aziridine analogue. The capability of oxazolinylazetidines to undergo different reaction patterns with organolithium bases supports the model termed “dynamic control of reactivity” of relevance in organolithium chemistry. It has been demonstrated that only 2,2-substituted oxazolinylazetidines with suitable stereochemical requirements could undergo C=N addition of organolithiums in non-coordinating solvents, leading to useful precursors of chiral (er > 95:5) ketoazetidines.
Antiinflammation Derived Suzuki-Coupled Fenbufens as COX-2 Inhibitors: Minilibrary Construction and Bioassay
Authors: Farn Shiou-Shiow , Lai Yen-Buo , Hua Kuo-Fong , Chen Hsiang-Ping , Yu Tzu-Yi , Lo Sheng-Nan , Shen Li-Hsin , Sheu Rong-Jiun , Yu Chung-Shan
Published: April 2022

A small fenbufen library comprising 18 compounds was prepared via Suzuki Miyara coupling. The five-step preparations deliver 9–17% biphenyl compounds in total yield. These fenbufen analogs exert insignificant activity against the IL-1 release as well as inhibiting cyclooxygenase 2 considerably. Both the para-amino and para-hydroxy mono substituents display the most substantial COX-2 inhibition, particularly the latter one showing a comparable activity as celecoxib. The most COX-2 selective and bioactive disubstituted compound encompasses one electron-withdrawing methyl and one electron-donating fluoro groups in one arene. COX-2 is selective but not COX-2 to bioactive compounds that contain both two electron-withdrawing groups; disubstituted analogs with both resonance-formable electron-donating dihydroxy groups display high COX-2 activity but inferior COX-2 selectivity. In silico simulation and modeling for three COX-2 active—p-fluoro, p-hydroxy and p-amino—fenbufens show a preferable docking to COX-2 than COX-1. The most stabilization by the p-hydroxy fenbufen with COX-2 predicted by theoretical simulation is consistent with its prominent COX-2 inhibition resulting from experiments.
Gastric Cancer Pre-Stage Detection and Early Diagnosis of Gastritis Using Serum Protein Signatures
Authors: Aziz Shahid , Rasheed Faisal , Zahra Rabaab , König Simone
Published: April 2022

Background: A gastric cancer (GC) diagnosis relies on histopathology. Endoscopy rates are increasing. Helicobacter pylori infection is a major GC risk factor. In an effort to elucidate abundant blood biomarkers, and potentially reduce the number of diagnostic surgical interventions, we investigated sera and biopsies from a cohort of 219 H. pylori positive and negative patients diagnosed with GC, gastritis, and ulcers. This allowed the comparative investigation of the different gastroduodenal diseases, and the exclusion of protein changes resulting from bacterial infection or inflammation of the gastric mucosa when searching for GC-dependent proteins. Methods: High-definition mass spectrometry-based expression analysis of tryptically digested proteins was performed, followed by multivariate statistical and network analyses for the different disease groups, with respect to H. pylori infection status. Significantly regulated proteins differing more than two-fold between groups were shortlisted, and their role in gastritis and GC discussed. Results: We present data of comparative protein analyses of biopsies and sera from patients suffering from mild to advanced gastritis, ulcers, and early to advanced GC, in conjunction with a wealth of metadata, clinical information, histopathological evaluation, and H. pylori infection status. We used samples from pre-malignant stages to extract prospective serum markers for early-stage GC, and present a 29-protein marker panel containing, amongst others, integrin β-6 and glutathione peroxidase. Furthermore, ten serum markers specific for advanced GC, independent of H. pylori infection, are provided. They include CRP, protein S100A9, and kallistatin. The majority of these proteins were previously discussed in the context of cancer or GC. In addition, we detected hypoalbuminemia and increased fibrinogen serum levels in gastritis. Conclusion: Two protein panels were suggested for the development of multiplex tests for GC serum diagnostics. For most of the elements contained in these panels, individual commercial tests are available. Thus, we envision the design of multi-protein assays, incorporating several to all of the panel members, in order to gain a level of specificity that cannot be achieved by testing a single protein alone. As their development and validation will take time, gastritis diagnosis based on the fibrinogen to albumin serum ratio may be a quick way forward. Its determination at the primary/secondary care level for early diagnosis could significantly reduce the number of referrals to endoscopy. Preventive measures are in high demand. The protein marker panels presented in this work will contribute to improved GC diagnostics, once they have been transferred from a research result to a practical tool.
Compact Ga2O3 Thin Films Deposited by Plasma Enhanced Atomic Layer Deposition at Low Temperature
Authors: Yang Yue , Zhang Xiao-Ying , Wang Chen , Ren Fang-Bin , Zhu Run-Feng , Hsu Chia-Hsun , Wu Wan-Yu , Wuu Dong-Sing , Gao Peng , Ruan Yu-Jiao , Lien Shui-Yang , Zhu Wen-Zhang
Published: April 2022

Amorphous Gallium oxide (Ga2O3) thin films were grown by plasma-enhanced atomic layer deposition using O2 plasma as reactant and trimethylgallium as a gallium source. The growth rate of the Ga2O3 films was about 0.6 Å/cycle and was acquired at a temperature ranging from 80 to 250 °C. The investigation of transmittance and the adsorption edge of Ga2O3 films prepared on sapphire substrates showed that the band gap energy gradually decreases from 5.04 to 4.76 eV with the increasing temperature. X-ray photoelectron spectroscopy (XPS) analysis indicated that all the Ga2O3 thin films showed a good stoichiometric ratio, and the atomic ratio of Ga/O was close to 0.7. According to XPS analysis, the proportion of Ga3+ and lattice oxygen increases with the increase in temperature resulting in denser films. By analyzing the film density from X-ray reflectivity and by a refractive index curve, it was found that the higher temperature, the denser the film. Atomic force microscopic analysis showed that the surface roughness values increased from 0.091 to 0.187 nm with the increasing substrate temperature. X-ray diffraction and transmission electron microscopy investigation showed that Ga2O3 films grown at temperatures from 80 to 200 °C were amorphous, and the Ga2O3 film grown at 250 °C was slightly crystalline with some nanocrystalline structures.
Recent Advancements of Polyaniline/Metal Organic Framework (PANI/MOF) Composite Electrodes for Supercapacitor Applications: A Critical Review
Authors: Vinodh Rajangam , Babu Rajendran Suresh , Sambasivam Sangaraju , Gopi Chandu V. V. Muralee , Alzahmi Salem , Kim Hee-Je , de Barros Ana Lucia Ferreira , Obaidat Ihab M.
Published: April 2022

Supercapacitors (SCs), also known as ultracapacitors, should be one of the most promising contenders for meeting the needs of human viable growth owing to their advantages: for example, excellent capacitance and rate efficiency, extended durability, and cheap materials price. Supercapacitor research on electrode materials is significant because it plays a vital part in the performance of SCs. Polyaniline (PANI) is an exceptional candidate for energy-storage applications owing to its tunable structure, multiple oxidation/reduction reactions, cheap price, environmental stability, and ease of handling. With their exceptional morphology, suitable functional linkers, metal sites, and high specific surface area, metal–organic frameworks (MOFs) are outstanding materials for electrodes fabrication in electrochemical energy storage systems. The combination of PANI and MOF (PANI/MOF composites) as electrode materials demonstrates additional benefits, which are worthy of exploration. The positive impacts of the two various electrode materials can improve the resultant electrochemical performances. Recently, these kinds of conducting polymers with MOFs composites are predicted to become the next-generation electrode materials for the development of efficient and well-organized SCs. The recent achievements in the use of PANI/MOFs-based electrode materials for supercapacitor applications are critically reviewed in this paper. Furthermore, we discuss the existing issues with PANI/MOF composites and their analogues in the field of supercapacitor electrodes in addition to potential future improvements.
Optical and Spin Properties of NV Center Ensembles in Diamond Nano-Pillars
Authors: Volkova Kseniia , Heupel Julia , Trofimov Sergei , Betz Fridtjof , Colom Rémi , MacQueen Rowan W. , Akhundzada Sapida , Reginka Meike , Ehresmann Arno , Reithmaier Johann Peter , Burger Sven , Popov Cyril , Naydenov Boris
Published: April 2022

Nitrogen-vacancy (NV) color centers in diamond are excellent quantum sensors possessing high sensitivity and nano-scale spatial resolution. Their integration in photonic structures is often desired, since it leads to an increased photon emission and also allows the realization of solid-state quantum technology architectures. Here, we report the fabrication of diamond nano-pillars with diameters up to 1000 nm by electron beam lithography and inductively coupled plasma reactive ion etching in nitrogen-rich diamonds (type Ib) with [100] and [111] crystal orientations. The NV centers were created by keV-He ion bombardment and subsequent annealing, and we estimate an average number of NVs per pillar to be 4300 ± 300 and 520 ± 120 for the [100] and [111] samples, respectively. Lifetime measurements of the NVs’ excited state showed two time constants with average values of τ1 ≈ 2 ns and τ2 ≈ 8 ns, which are shorter as compared to a single color center in a bulk crystal (τ ≈ 10 ns). This is probably due to a coupling between the NVs as well as due to interaction with bombardment-induced defects and substitutional nitrogen (P1 centers). Optically detected magnetic resonance measurements revealed a contrast of about 5% and average coherence and relaxation times of T2 [100] = 420 ± 40 ns, T2 [111] = 560 ± 50 ns, and T1 [100] = 162 ± 11 μs, T1 [111] = 174 ± 24 μs. These pillars could find an application for scanning probe magnetic field imaging.
Intracellular Trafficking and Distribution of Cd and InP Quantum Dots in HeLa and ML-1 Thyroid Cancer Cells
Authors: Zhang Min , Kim Daniel S. , Patel Rishi , Wu Qihua , Kim Kyoungtae
Published: April 2022

The study of the interaction of engineered nanoparticles, including quantum dots (QDs), with cellular constituents and the kinetics of their localization and transport, has provided new insights into their biological consequences in cancers and for the development of effective cancer therapies. The present study aims to elucidate the toxicity and intracellular transport kinetics of CdSe/ZnS and InP/ZnS QDs in late-stage ML-1 thyroid cancer using well-tested HeLa as a control. Our XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) viability assay (Cell Proliferation Kit II) showed that ML-1 cells and non-cancerous mouse fibroblast cells exhibit no viability defect in response to these QDs, whereas HeLa cell viability decreases. These results suggest that HeLa cells are more sensitive to the QDs compared to ML-1 cells. To test the possibility that transporting rates of QDs are different between HeLa and ML-1 cells, we performed a QD subcellular localization assay by determining Pearson’s Coefficient values and found that HeLa cells showed faster QDs transporting towards the lysosome. Consistently, the ICP-OES test showed the uptake of CdSe/ZnS QDs in HeLa cells was significantly higher than in ML-1 cells. Together, we conclude that high levels of toxicity in HeLa are positively correlated with the traffic rate of QDs in the treated cells.
Stabilization and Dispersion of OSA Starch-Coated Titania Nanoparticles in Kappa-Carrageenan-Based Solution
Authors: Chen Xingyu , Wu Kai , Zeng Sili , Chen Da , Yao Lingyun , Song Shiqing , Wang Huatian , Sun Min , Feng Tao
Published: April 2022

Titania is a white pigment used widely in papermaking, paints and cosmetic industries. Dispersion and stabilization of high concentration titania in water-based system remains a great bottleneck in industry nowadays, because aggregation of titania nanoparticles results in severe adverse effects to gloss, opacity, tint strength, color distribution and storage stability of end products. Because kappa-carrageenan (κ-CG) has excellent rheological properties such as emulsification, gelation, stability and so on, it has the ability to form gel and increase the viscosity of aqueous solution. In this work, Octenyl succinic anhydride (OSA) starch was utilized as wall material to encapsulate titania pigments using electrostatic spray drying processing. Transmission electron microscopy (TEM) showed that titania pigments were coated by OSA starch, with a final form of nanoparticle. Accelerating stability test found that around 60% OSA starch–titania particles were stably dispersed in κ-CG-based solution. All materials used in this work were natural ingredient, which would be preferred by cosmetic industry and consumers. The technique used in the present study could potentially be extended to other pigments for similar purpose.
Optical Properties of Polyisocyanurate–Polyurethane Aerogels: Study of the Scattering Mechanisms
Authors: Merillas Beatriz , Martín-de León Judith , Villafañe Fernando , Rodríguez-Pérez Miguel Ángel
Published: April 2022

Highly transparent polyisocyanurate–polyurethane (PUR–PIR) aerogels were synthesized, and their optical properties were studied in detail. After determining the density and structural parameters of the manufactured materials, we analyzed their optical transmittance. It was demonstrated that the catalyst content used to produce the aerogels can be employed to tune the internal structure and optical properties. The results show that the employment of lower catalyst amounts leads to smaller particles forming the aerogel and concomitantly to higher transmittances, which reach values of 85% (650 nm) due to aerogel particles acting as scattering centers. Thus, it was found that the lower this size, the higher the transmittance. The effect of the sample thickness on the transmittance was studied through the Beer–Lambert law. Finally, the scattering mechanisms involved in the light attenuation were systematically evaluated by measuring a wide range of light wavelengths and determining the transition between Rayleigh and Mie scattering when the particles were larger. Therefore, the optical properties of polyurethane aerogels were studied for the first time, opening a wide range of applications in building and energy sectors such as glazing windows.
Context-Dependent Regulation of Gene Expression by Non-Canonical Small RNAs
Authors: Plawgo Kinga , Raczynska Katarzyna Dorota
Published: April 2022

In recent functional genomics studies, a large number of non-coding RNAs have been identified. It has become increasingly apparent that noncoding RNAs are crucial players in a wide range of cellular and physiological functions. They have been shown to modulate gene expression on different levels, including transcription, post-transcriptional processing, and translation. This review aims to highlight the diverse mechanisms of the regulation of gene expression by small noncoding RNAs in different conditions and different types of human cells. For this purpose, various cellular functions of microRNAs (miRNAs), circular RNAs (circRNAs), snoRNA-derived small RNAs (sdRNAs) and tRNA-derived fragments (tRFs) will be exemplified, with particular emphasis on the diversity of their occurrence and on the effects on gene expression in different stress conditions and diseased cell types. The synthesis and effect on gene expression of these noncoding RNAs varies in different cell types and may depend on environmental conditions such as different stresses. Moreover, noncoding RNAs play important roles in many diseases, including cancer, neurodegenerative disorders, and viral infections.
Monotropein Improves Dexamethasone-Induced Muscle Atrophy via the AKT/mTOR/FOXO3a Signaling Pathways
Authors: Wang Piao , Kang Seok Yong , Kim Su Jin , Park Yong-Ki , Jung Hyo Won
Published: May 2022

The present study aimed to investigate the effects of monotropein (MON) on improving dexamethasone (DEX)-induced muscle atrophy in mice and C2C12 mouse skeletal muscle cells. The body weights, grip strengths, and muscle weights of mice were assessed. The histological change in the gastrocnemius tissues was also observed through H&E staining. The expression of myosin heavy chain (MyHC), muscle ring finger 1 (MuRF1), and muscle atrophy F-box (Atrogin1) and the phosphorylation of AKT, mTOR, and FOXO3a in the muscle tissues of mice and C2C12 myotubes were analyzed using Western blotting. MON improved muscle atrophy in mice and C2C12 myotubes by regulating catabolic states via the AKT/mTOR/FOXO3a signaling pathways, and enhanced muscle function by the increases of muscle mass and strength in mice. This suggests that MON could be used for the prevention and treatment of muscle atrophy in patients.
Effect of a Combination of Magnesium, B Vitamins, Rhodiola, and Green Tea (L-Theanine) on Chronically Stressed Healthy Individuals—A Randomized, Placebo-Controlled Study
Authors: Noah Lionel , Morel Veronique , Bertin Claire , Pouteau Etienne , Macian Nicolas , Dualé Christian , Pereira Bruno , Pickering Gisèle
Published: May 2022

The effect of a combination of magnesium, vitamins B6, B9, B12, rhodiola and green tea/L-theanine (Mg-Teadiola) on stress was evaluated in chronically stressed, otherwise healthy individuals. Effects on stress-related quality-of-life parameters (sleep and perception of pain) were also explored. Adults with stress for ≥1 month, scoring ≥14 points on the Depression Anxiety Stress Scale (DASS)-42 questionnaire, were randomized (1:1) to receive oral Mg-Teadiola (n = 49) or a placebo (n = 51), for 28 days, with a follow-up assessment on Day 56 (NCT04391452). The primary endpoint was the change in the DASS-42 stress score from baseline to Day 28 with Mg-Teadiola versus placebo. The DASS-42 stress scores significantly decreased from baseline to Day 28 with Mg-Teadiola versus placebo (effect size, 0.29; 95% CI [0.01, 0.57]; p = 0.04). Similar reductions were observed on Day 14 (p = 0.006) and Day 56 (p = 0.02). A significant reduction in sensitivity to cold pain (p = 0.01) and a trend for lower sensitivity to warm pain was observed (p = 0.06) on Day 28. Improvements in daytime dysfunction due to sleepiness (Pittsburgh Sleep Quality Index-7 component score) were reported on Day 28, and were significant on Day 56 (p < 0.001). Mg-Teadiola is effective in managing stress in otherwise healthy individuals. Its beneficial effects on sleep and pain perception need further investigation.
Bioelectrical Impedance Analysis for the Assessment of Body Composition in Sarcopenia and Type 2 Diabetes
Authors: Sbrignadello Stefano , Göbl Christian , Tura Andrea
Published: May 2022

Sarcopenia is emerging as a severe complication in type 2 diabetes (T2DM). On the other hand, it has been documented that nutritional aspects, such as insufficient protein or total energy intake, increase sarcopenia risk. The analysis of body composition is a relevant approach to assess nutritional status, and different techniques are available. Among such techniques, bioelectrical impedance analysis (BIA) is particularly interesting, since it is non-invasive, simple, and less expensive than the other techniques. Therefore, we conducted a review study to analyze the studies using BIA for body composition analysis in T2DM patients with sarcopenia or at risk of catching it. Revised studies have provided important information concerning relationships between body composition parameters (mainly muscle mass) and other aspects of T2DM patients’ conditions, including different comorbidities, and information on how to avoid muscle mass deterioration. Such relevant findings suggest that BIA can be considered appropriate for body composition analysis in T2DM complicated by sarcopenia/muscle loss. The wide size of the patients’ cohort in many studies confirms that BIA is convenient for clinical applications. However, studies with a specific focus on the validation of BIA, in the peculiar population of patients with T2DM complicated by sarcopenia, should be considered.
Development of the Hedonic Overeating–Questionnaire (HEDO–Q)
Authors: Hilbert Anja , Witte Veronica , Meule Adrian , Braehler Elmar , Kliem Soeren
Published: May 2022

Addictive-like eating is prevalent, but a clear conceptualization and operationalization outside of an addiction framework is lacking. By adopting a biopsychological framework of food reward, this study sought to develop and evaluate a brief self-report questionnaire for the trait assessment of hedonic overeating and dyscontrol. Items in the Hedonic Overeating–Questionnaire (HEDO–Q) were constructed following a rational approach and psychometrically evaluated in a large random sample from the German population (N = 2531). A confirmatory factor analysis supported the unidimensional nature of the six-item HEDO–Q with the three postulated components of wanting, liking, and dyscontrol. Psychometric properties were favorable with good corrected item-total correlations, acceptable item difficulty and homogeneity, and high internal consistency. Population norms were provided. The HEDO–Q revealed strict measurement invariance for sex and partial invariance for age and weight status. Discriminant validity was demonstrated in distinguishing participants with versus without eating disturbances or obesity. Associations with the established measures of eating disorder and general psychopathology supported the convergent and divergent validity of the HEDO–Q. This first evaluation indicates good psychometric properties of the HEDO–Q in the general population. Future validation work is warranted on the HEDO–Q’s stability, sensitivity to change, and predictive and construct validity.
Screening for Low Energy Availability in Male Athletes: Attempted Validation of LEAM-Q
Authors: Lundy Bronwen , Torstveit Monica K. , Stenqvist Thomas B. , Burke Louise M. , Garthe Ina , Slater Gary J. , Ritz Christian , Melin Anna K.
Published: May 2022

A questionnaire-based screening tool for male athletes at risk of low energy availability (LEA) could facilitate both research and clinical practice. The present options rely on proxies for LEA such screening tools for disordered eating, exercise dependence, or those validated in female athlete populations. in which the female-specific sections are excluded. To overcome these limitations and support progress in understanding LEA in males, centres in Australia, Norway, Denmark, and Sweden collaborated to develop a screening tool (LEAM-Q) based on clinical investigations of elite and sub-elite male athletes from multiple countries and ethnicities, and a variety of endurance and weight-sensitive sports. A bank of questions was developed from previously validated questionnaires and expert opinion on various clinical markers of LEA in athletic or eating disorder populations, dizziness, thermoregulation, gastrointestinal symptoms, injury, illness, wellbeing, recovery, sleep and sex drive. The validation process covered reliability, content validity, a multivariate analysis of associations between variable responses and clinical markers, and Receiver Operating Characteristics (ROC) curve analysis of variables, with the inclusion threshold being set at 60% sensitivity. Comparison of the scores of the retained questionnaire variables between subjects classified as cases or controls based on clinical markers of LEA revealed an internal consistency and reliability of 0.71. Scores for sleep and thermoregulation were not associated with any clinical marker and were excluded from any further analysis. Of the remaining variables, dizziness, illness, fatigue, and sex drive had sufficient sensitivity to be retained in the questionnaire, but only low sex drive was able to distinguish between LEA cases and controls and was associated with perturbations in key clinical markers and questionnaire responses. In summary, in this large and international cohort, low sex drive was the most effective self-reported symptom in identifying male athletes requiring further clinical assessment for LEA.