bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Effect of Age on the Reproductive and Stress Physiology of Octopus bimaculoides Using Dermal Hormones
Authors: Chancellor Stephanie , Grasse Bret , Sakmar Taylor , Scheel David , Brown Joel S. , Santymire Rachel M.
Published: October 2023

Our goal was to validate the use of dermal swabs to evaluate both reproductive and stress physiology in the California two-spot octopus, Octopus bimaculoides. Our objectives were to (1) use dermal swabs to evaluate glucocorticoids and reproductive hormones of O. bimaculoides; (2) determine the influence of life stage on hormone production (glucocorticoids in all individuals; testosterone, estrogen, and progesterone in females; and testosterone in males) of reproductive (n = 4) and senescent (n = 8) individuals to determine the effect of age on hormonal patterns; and (3) determine whether these hormones change significantly in response to an acute stressor. For the stress test, individuals were first swabbed for a baseline and then chased around the aquarium with a net for 5 min. Afterward, individuals were swabbed for 2 h at 15 min intervals to compare to the pre-stress test swab. Reproductive individuals responded to the stressor with a 2-fold increase in dermal cortisol concentrations at 15 and 90 min. Six of the eight senescent individuals did not produce a 2-fold increase in dermal cortisol concentrations. Reproductive individuals had significantly higher sex hormone concentrations compared to senescent individuals (progesterone and estradiol measured in females, and testosterone for both sexes). After the stressor, only reproductive males produced a 2-fold increase in dermal testosterone concentrations, while sex hormones in females showed no change. The stress hormone cortisol was significantly higher in senescent than in reproductive individuals, independent of sex. Dermal corticosterone concentrations were highest in senescent females followed by senescent males, and lowest in reproductive individuals regardless of sex. Dermal swabs provide an effective and noninvasive means for evaluating octopus hormones. Application of these indicators may be imperative as cephalopods are more commonly cultured in captivity for experimentation, display, and consumption.
Genotype by Environment Interactions (G*E) of Chickens Tested in Ethiopia Using Body Weight as a Performance Trait
Authors: de Kinderen Maud A. J. , Sölkner Johann , Mészáros Gábor , Alemu Setegn W. , Esatu Wondmeneh , Bastiaansen John W. M. , Komen Hans , Dessie Tadelle
Published: October 2023

Ethiopia is a developing nation that could highly benefit from securing food via improved smallholder poultry farming. To support farmer and breeding decisions regarding which chicken strain to use in which Ethiopian environment, G*E analyses for body weight (BW) of growing male and female chickens were conducted. Research questions were (1) if a G*E is present for BW and (2) which strain performs best in which environment in terms of predicted BW. Analyses were performed using predicted BW at four different ages (90, 120, 150, and 180 days) of five strains (Horro, Koekoek, Kuroiler, Sasso-Rhode Island Red (S-RIR), and Sasso) tested in five Ethiopian regions (Addis Ababa, Amhara, Oromia, South Region, and Tigray) that are part of three Agro-Ecological Zones (AEZ) (cool humid, cool sub-humid, and warm semi-arid). The indigenous Horro strain was used as a control group to compare four other introduced tropically adapted strains. The dataset consisted of 999 female and 989 male farm-average BW measurements. G*E was strongly present (p < 0.001) for all combinations of strain and region analyzed. In line with previous research, Sasso was shown to have the highest predicted BW, especially at an early age, followed by Kuroiler. Horro had the lowest predicted BW at most ages and in most regions, potentially due to its young breeding program. The highest predicted BW were observed in Tigray, Oromia, and Amhara regions, which are in the main part of the cool sub-humid AEZ.
The Impact of Antibiotic Prophylaxis on a Retrospective Cohort of Hospitalized Patients with COVID-19 Treated with a Combination of Steroids and Tocilizumab
Authors: Membrillo de Novales Francisco Javier , Ramírez-Olivencia Germán , Mata Forte Maj. Tatiana , Zamora Cintas María Isabel , Simón Sacristán Maj. María , Sánchez de Castro María , Estébanez Muñoz Miriam
Published: October 2023

Objectives: In the context of COVID-19, patients with a severe or critical illness may be more susceptible to developing secondary bacterial infections. This study aims to investigate the relationship between the use of prophylactic antibiotic therapy and the occurrence of bacterial or fungal isolates following the administration of tocilizumab in hospitalized COVID-19 patients who had previously received steroids during the first and second waves of the pandemic in Spain. Methods: This retrospective observational study included 70 patients hospitalized with COVID-19 who received tocilizumab and steroids between January and December 2020. Data on demographics, comorbidities, laboratory tests, microbiologic results, treatment, and outcomes were collected from electronic health records. The patients were divided into two groups based on the use of antibiotic prophylaxis, and the incidence of bacterial and fungal colonizations/infections was analyzed. Results: Among the included patients, 45 patients received antibiotic prophylaxis. No significant clinical differences were observed between the patients based on prophylaxis use regarding the number of clinically diagnosed infections, ICU admissions, or mortality rates. However, the patients who received antibiotic prophylaxis showed a higher incidence of colonization by multidrug-resistant bacteria compared to that of the subgroup that did not receive prophylaxis. The most commonly isolated microorganisms were Candida albicans, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis. Conclusions: In this cohort of hospitalized COVID-19 patients treated with tocilizumab and steroids, the use of antibiotic prophylaxis did not reduce the incidence of secondary bacterial infections. However, it was associated with an increased incidence of colonization by multidrug-resistant bacteria.
An Artificial Intelligence-Based Method for Crack Detection in Engineering Facilities around Subways
Authors: Ding Zhikun , Luo Liwei , Wang Xinrui , Liu Yongqi , Zhang Wei , Wu Huanyu
Published: October 2023

While the construction and operation of subways have brought convenience to commuters, it has also caused ground subsidence and cracks of facilities around subways. The industry mainly adopts traditional manual detection methods to monitor these settlements and cracks. The current approaches have difficulties in achieving all-weather, all-region dynamic monitoring, increasing the traffic burden of the city during the monitoring work. The study aims to provide a large-scale settlement detection approach based on PS-InSAR for the monitoring of subway facilities. Meanwhile, this paper proposes a crack detection method that is based on UAVs and the VGG16 algorithm to quantify the length and width of cracks. The experimental data of Shenzhen University Section of Metro Line 9 are used to verify the proposed settlement model and to illustrate the monitoring process. The developed model is innovative in that it can monitor the settlement of large-scale facilities around the subway with high accuracy around the clock and automatically identify and quantify the cracks in the settled facilities around the subway.
Evolution Characteristics of Plastic Zone in Jointed Rock Mass of High-Temperature Hydraulic Tunnel
Authors: Li Kangbin , Jiang Haibo , Wei Gang , Xiang Pengfei
Published: October 2023

The evolution characteristics and the extent of the plastic zone in rock mass can reflect the failure characteristics and destruction degree of a hydraulic tunnel. In this research, we derived the equation of a plastic zone range based on a gateway-like structure high-temperature tunnel through a theoretical analysis. On-site monitoring and discrete element model simulation were combined to analyze the temperature field law and plastic zone evolution characteristics of the jointed rock mass at high temperatures. The results show that the joints affect the temperature field variation in rock mass, and the vertical and horizontal joint groups pose significantly greater influence than the inclined joint group on temperature field. The sensitivity of the joint internal friction angle and thermal expansion coefficient to the range of the plastic zone is relatively small. Under various joint spacings, the influence of horizontal and inclined joint groups on the plastic zone morphology decreases, while the vertical joint group exhibits an incremental influence on the plastic zone morphology. Similar to the influence of the temperature field, the vertical and horizontal joint groups have a significantly greater influence on the plastic zone range than the inclined joint group. Under various rock mass temperatures, the plastic zone in rock mass results in the occurrence of uneven expansions along the direction of the joint dip angle, which changes the potential failure direction of rock mass and increases the potential destruction degree of rock mass, whereas it exhibits a smaller uniform expansion perpendicular to the joint dip angle, and the boundary of the plastic zone coincides with the joint surface, relatively hindering the potential destruction degree of rock mass. The research results of this study have certain reference values for the stability control of jointed rock mass in high-temperature hydraulic tunnels.
WSREB Mechanism: Web Search Results Exploration Mechanism for Blind Users
Authors: Naseer Snober , Rashid Umer , Saddal Maha , Khan Abdur Rehman , Abbas Qaisar , Daadaa Yassine
Published: October 2023

In the contemporary digital landscape, web search functions as a pivotal conduit for information dissemination. Nevertheless, blind users (BUs) encounter substantial barriers in leveraging online services, attributable to intrinsic deficiencies in the information structure presented by online platforms. A critical analysis reveals that a considerable segment of BUs perceive online service access as either challenging or unfeasible, with only a fraction of search endeavors culminating successfully. This predicament stems largely from the linear nature of information interaction necessitated for BUs, a process that mandates sequential content relevancy assessment, consequently imposing cognitive strain and fostering information disorientation. Moreover, the prevailing evaluative metrics for web service efficacy—precision and recall—exhibit a glaring oversight of the nuanced behavioral and usability facets pertinent to BUs during search engine design. Addressing this, our study introduces an innovative framework to facilitate information exploration, grounded in the cognitive principles governing BUs. This framework, piloted using the Wikipedia dataset, seeks to revolutionize the search result space through categorical organization, thereby enhancing accessibility for BUs. Empirical and usability assessments, conducted on a cohort of legally blind individuals (N = 25), underscore the framework’s potential, demonstrating notable improvements in web content accessibility and system usability, with categorical accuracy standing at 84% and a usability quotient of 72.5%. This research thus holds significant promise for redefining web search paradigms to foster inclusivity and optimized user experiences for BUs.
Simulations of Deformation Failure Process and Refinement of Reinforcement Scheme in the Dabenliu Quarry Slope Using Discontinuous Deformation Analysis
Authors: Hou Wei-Hua , Xiong Feng , Zhang Qi-Hua
Published: October 2023

For this study, the geological engineering features and possible failure modes of the Dabenliu quarry slope in the Jinping-I Hydropower Station were qualitatively analyzed before a method for setting viscous boundary and an algorithm for modeling pre-stressed cables were embedded into a DDA (Discontinuous Deformation Analysis) computer code to analyze the deformation of the slope under seismic loading. Our simulation results revealed that the middle and upper parts of the slope slipped along the bedding joints (interlayer shear zones) and that the lower part buckled and collapsed after the slope was excavated. This is a typical slipping–buckling failure mode characterized by upper-slipping followed by lower-buckling. Based on the distribution of the simulated internal force of the anchor cables, the reinforcement scheme was adjusted by strengthening the support for the middle and lower parts of the slope, whereas the length and pre-stress of the anchor cables were reduced for the upper part of the slope. The adjusted reinforcement scheme can ensure the stability of the slope under the action of a magnitude 7 earthquake, and the slope may lose stability with no evident collapse under the action of a magnitude 8 earthquake. Finally, the simulation results were verified via a comparison with the monitoring data regarding the slope.
Metabolically Active Microbial Communities in Oilfields: A Systematic Review and Synthesis of RNA Preservation, Extraction, and Sequencing Methods
Authors: Gomes Rosimeire Floripes , García Glen Jasper Yupanqui , Dutra Joyce da Cruz Ferraz , Cardoso Mariana Santos , Costa Eduardo Almeida , de Abreu Waldow Vinicius , Groposo Claudia Julia , Akamine Rubens Nobumoto , de Sousa Maira Paula , Figueiredo Henrique , Azevedo Vasco Ariston de Carvalho , Góes-Neto Aristóteles
Published: October 2023

Characterizing metabolically active microorganisms using RNA-based methods is a crucial tool for monitoring and mitigating operational issues, such as oil biodegradation and biocorrosion of pipelines in the oil and gas industry. Our review, a pioneering study, addresses the main methods used to preserve, isolate, and sequence RNA from oilfield samples and describes the most abundant metabolically active genera studied. Using the MEDLINE/PubMed, PubMed Central, Scopus, and Web of Science databases, 2.561 potentially eligible records were identified. After screening, 20 studies were included in our review, underscoring the scarcity of studies related to the subject. Data were extracted and reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). These studies evaluated different samples, including produced water (PW), injection water (IW), solid deposits (SD), oil (OIL), and oily sludge (OS) collected from oilfields located in Australia, China, India, Mexico, and the United Arab Emirates. Environmental samples accounted for 55% of the studies, while enriched cultures and microbial consortia represented 35% and 15% of studies, respectively. PW was the most frequently studied sample, comprising 72% of all samples. Filtration and centrifugation were the only processes employed to concentrate the biomass present in samples. For RNA preservation, the most used method was a solution composed of 95:5 v/v ethanol/TRIzol, while for RNA isolation, the TRIzol reagent was the most cited. The Sanger sequencing method was used in all studies evaluating functional genes (alkB, dsrA, aprA, assA, and mcrA), and the Next-Generation Sequencing (NGS) method was employed in studies for sequencing transcripts of the 16S rRNA gene and metatranscriptomes. Pseudomonas (16S rRNA = PW: 2%; IW: 8%; metatranscriptome = PW: 20%) and Acinetobacter (16S rRNA = PW: 1%; IW: 4%; metatranscriptome = PW: 17%) were the most abundant genera. This study outlined the primary methods employed in researching metabolically active microorganisms. These data provide a foundation for future research. However, it is essential to note that we cannot yet determine the most effective method. We hope that this study will inspire further research related to the standardization of RNA preservation, extraction, and sequencing methods and significantly contribute to our understanding of active microbial communities in oilfields.
Accelerated Burn Healing in a Mouse Experimental Model Using α-Gal Nanoparticles
Authors: Galili Uri
Published: October 2023

Macrophages play a pivotal role in the process of healing burns. One of the major risks in the course of burn healing, in the absence of regenerating epidermis, is infections, which greatly contribute to morbidity and mortality in such patients. Therefore, it is widely agreed that accelerating the recruitment of macrophages into burns may contribute to faster regeneration of the epidermis, thus decreasing the risk of infections. This review describes a unique method for the rapid recruitment of macrophages into burns and the activation of these macrophages to mediate accelerated regrowth of the epidermis and healing of burns. The method is based on the application of bio-degradable “α-gal” nanoparticles to burns. These nanoparticles present multiple α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R), which bind the abundant natural anti-Gal antibody that constitutes ~1% of immunoglobulins in humans. Anti-Gal/α-gal nanoparticle interaction activates the complement system, resulting in localized production of the complement cleavage peptides C5a and C3a, which are highly effective chemotactic factors for monocyte-derived macrophages. The macrophages recruited into the α-gal nanoparticle-treated burns are activated following interaction between the Fc portion of anti-Gal coating the nanoparticles and the multiple Fc receptors on macrophage cell membranes. The activated macrophages secrete a variety of cytokines/growth factors that accelerate the regrowth of the epidermis and regeneration of the injured skin, thereby cutting the healing time by half. Studies on the healing of thermal injuries in the skin of anti-Gal-producing mice demonstrated a much faster recruitment of macrophages into burns treated with α-gal nanoparticles than in control burns treated with saline and healing of the burns within 6 days, whereas healing of control burns took ~12 days. α-Gal nanoparticles are non-toxic and do not cause chronic granulomas. These findings suggest that α-gal nanoparticles treatment may harness anti-Gal for inducing similar accelerated burn healing effects also in humans.
Determination of Climate-Based Daylight Metrics under 15 CIE (International Commission on Illumination) Standard Skies and Three Representative Skies
Authors: Li Shuyang , Li Danny , Chen Wenqiang , Tsang Ernest , Lou Siwei , Wang Zhenyu
Published: October 2023

Daylighting serves as a crucial sustainable strategy in assessing the built environment. Climate-based daylight metrics (CBDMs) have been widely employed to evaluate the dynamic performance of daylight. However, conventional CBDM calculations heavily rely on time-consuming full-scale computer simulations. In addition, the CBDMs need representative annual daylight data that are essential for CBDM analysis, and can pose challenges in many locations. Even when suitable daylight data are available, they may not accurately reflect current trends and conditions. This study aims to determine the various CBDMs using both the 15 CIE Standard Skies and the three representative skies specific to Hong Kong. All data were simulated from a software named RADIANCE (version 5.3). The R2s of the CBDMs under both the 15 CIE Standard Skies and the three representative skies were more than 0.89, and the MBEs and RMSEs were not more than 5.4% and 9.1%, respectively, when the outdoor illuminance measured in 2004 was employed. The findings could be adopted for other locations where the required daylight parameters were not systematically recorded.
A Thia-Analogous Indirubin N-Glycoside Disrupts Mitochondrial Function and Causes the Death of Human Melanoma and Cutaneous Squamous Cell Carcinoma Cells
Authors: Wendt Franziska , Wittig Felix , Rupprecht Anne , Ramer Robert , Langer Peter , Emmert Steffen , Frank Marcus , Hinz Burkhard
Published: October 2023

Skin cancer is the most common malignant disease worldwide and, therefore, also poses a challenge from a pharmacotherapeutic perspective. Derivatives of indirubin are an interesting option in this context. In the present study, the effects of 3-[3′-oxo-benzo[b]thiophen-2′-(Z)-ylidene]-1-(β-d-glucopyranosyl)-oxindole (KD87), a thia-analogous indirubin N-glycoside, on the viability and mitochondrial properties of melanoma (A375) and squamous cell carcinoma cells (A431) of the skin were investigated. In both cell lines, KD87 caused decreased viability, the activation of caspases-3 and -7, and the inhibition of colony formation. At the mitochondrial level, a concentration-dependent decrease in both the basal and ATP-linked oxygen consumption rate and in the reserve capacity of oxidative respiration were registered in the presence of KD87. These changes were accompanied by morphological alterations in the mitochondria, a release of mitochondrial cytochrome c into the cytosol and significant reductions in succinate dehydrogenase complex subunit B (SDHB, subunit of complex II) in A375 and A431 cells and NADH:ubiquinone oxidoreductase subunit B8 (NDUFB8, subunit of complex I) in A375 cells. The effect of KD87 was accompanied by a significant upregulation of the enzyme heme oxygenase-1, whose inhibition led to a partial but significant reduction in the metabolic-activity-reducing effect of KD87. In summary, our data show a mitochondria-targeting effect of KD87 as part of the cytotoxic effect of this compound on skin cancer cells, which should be considered in future studies with this class of compounds.
Efficiency of an Alternative Physical Education Program for the Lower Grades of Elementary School Children
Authors: Marković Jovan , Bubanj Saša , Šekeljić Goran , Pavlović Slobodan , Radenković Marko , Stanković Dušan , Petković Emilija , Aksović Nikola , Radenković Oliver , Preljević Adem , Bjelica Bojan , Petrović Vladan , Sinanović Šćepan , Tomović Milena
Published: October 2023

(1) Introduction: This research was conducted with the aim of assessing whether an alternative physical education (PE) program could effectively substitute for the traditional PE curriculum and aid in accomplishing the essential goals and objectives of PE among younger elementary school children. (2) Materials and Methods: This longitudinal 6-month study included third-grade elementary school children of both genders, who were healthy, lived in urban areas, and were involved in an alternative PE program, as well as the regular PE classes. The sample of participants comprised 214 students, with 105 participants in the experimental group and 109 participants in the control group, who underwent pre- and post-intervention measurements. For the purposes of this study, 11 variables were applied to assess the parameters of growth and development, motor abilities, and physical and health education. MANCOVA and ANCOVA methods were used to determine the effects resulting from the alternative and regular PE programs, and differences between the groups, respectively. The data are reported as the mean and standard deviations, and were analyzed using the statistical package SPSS version 20.0 (SPSS Inc., Armonk, NY, USA). (3) Results: Based on the research results obtained for motor abilities and physical and health education, it was concluded that both the alternative and regular PE programs had positive effects in achieving the goals and objectives of PE, but without statistical significance at the multivariate and univariate levels. The differences between the two groups were found to be negligible (effect size, ES < 0.2). (4) Discussion: Sports and PE have distinct objectives and approaches. While sports emphasize competition and winning, PE aims to impart fundamental skills and knowledge, prioritizing inclusivity among younger students. Success in PE is measured by the number of students meeting these goals, potentially affecting both talented and struggling learners. (5) Conclusions: The results obtained from the conducted research indicate that both the alternative PE program and the regular PE program influence changes in motor abilities and physical and health education to a limited extent. The alternative PE program proposed in this study, with its modifications to the structure of existing, regular PE program, can fully replace the latter in schools that do not meet the required spatial and material standards.
Influence of Cathodic Polarization on Plasma Electrolytic Oxidation of Magnesium and AZ31 and AZ91 Magnesium Alloys
Authors: Le Zhengzhou , Liu Zihua , He Xiaorui , Cheng Yulin , Hu Panfeng , Cheng Yingliang
Published: October 2023

In this study, the influence of cathodic polarization on the plasma electrolytic oxidation (PEO) behaviors of pure magnesium and AZ31 and AZ91 magnesium alloys with varied Al alloying contents was systematically examined in a dilute alkaline silicate electrolyte by adjusting the cathodic-to-anodic current density ratio (R = jc/ja) from 0 to 3.2. The results show that moderate cathodic polarization (R = 0.6) led to the thickest coatings on the Mg and Mg alloys, and the coatings grew in an outward-and-inward mode compared with the inward growth at R = 0. Excessive cathodic polarization (high R ratios) differently influenced the PEO behaviors of the magnesium alloys. For the pure magnesium and AZ31 alloy, the coatings blistered or peeled off when the R ≥ 0.9. However, the tolerance to cathodic polarization was significantly improved for the AZ91 Mg alloy. The coatings were undamaged even with the highest R ratio of 3.2, and their compactness was further improved as the R ratio increased to 0.9 and 1.2. An increase in cathodic polarization led to a reduction in the anodic potential and spark softening but did not result in an improvement in the coating quality. Optical emission spectroscopy identified two spectral lines at 559.79 and 570.11 nm, which are assigned to the Mg species but not found in databases or the literature. The corrosion and wear resistance of the PEO coatings were also investigated. The coating formed on the AZ91 magnesium alloy at R = 1.2 displayed the narrowest wear track due to its high compactness.
Double Duty: Complete Pathologic Response of Two Colonic Primaries with Mosaicism of a Novel MLH1 Mutation to Neoadjuvant Pembrolizumab
Authors: Preti Beatrice , Schenkel Laila , Cecchini Matthew , Romagnoli Tommaso , Sanatani Michael Susmoy , French Karissa , Colquhoun Patrick , Vincent Mark David
Published: October 2023

We present a fascinating case of a 57-year-old male with a novel mutation in MLH1 (MLH1:c.1288G > T, p.(Glu430*)), who presented with two synchronous colonic tumours, initially deemed unresectable, and experienced a complete pathological response on neoadjuvant pembrolizumab. Extensive genetic testing revealed post-zygotic mosaicism from the novel mutation.
Two Steps for Improving Reduced Graphene Oxide/Activated Durian Shell Carbon Composite by Hydrothermal and 3-D Ball Milling Process for Symmetry Supercapacitor Device
Authors: Ngamjumrus Nantikron , Silakaew Kanyapak , Thompho Somphob , Sriwong Chaval , Ruttanapun Chesta
Published: October 2023

Durian shell waste was used to fabricate activated carbon (AC) using a hydrothermal process and three-dimensional (3-D) ball milling. Reduced graphene oxide (rGO) was composited with activated durian shell carbon (DC) to enhance the electrochemical properties for fabricating a supercapacitor (SC) device. Scanning electron microscopic (SEM) examination of the AC from hydrothermally processed durian shell carbon (AC–HDC) and AC–HDC that was 3D ball milled for 15 min (rGO/AC–HDC–3D15M) showed compacted and uniformly distributed particles with good porosity. The rGO/AC–HDC–3D15M sample exhibited high specific surface area (SSA) using the Brunauer–Emmett–Teller (BET) methodology, 2311 m2/g, and an average pore size of 1.88 nm. Electrochemical results showed that the rGO/AC–HDC–3D15M sample had the highest specific capacitance (Cs) of 545.78 F/g, power density (Pd) of 260.834 W/kg and energy density (Ed) of 60.834 Wh/kg. A coin cell SC device using an rGO/AC–HDC3D15M electrode with a 3M KOH electrolyte exhibited a high Cs of 65.585 F/g with a high energy density of 5.123 W h/kg and power density of 47.286 W/kg. Thus, the novelty of this manuscript is that (1) the structure of the rGO/AC–HDC–3D15M composite could promote fast ionic and electronic migration during charging and discharging and (2) a rGO/AC–HDC–3D15M composite, which showed electric double-layer capacitor (EDLC) could produce a positive synergistic effect for efficient electrochemical reactions. Moreover, the high surface area of the rGO/AC–HDC–3D15M composite may mitigate the volume expansion of electrodes during cycling. Thus, this work shows that an rGO/AC–HDC–3D15M composite prepared using a hydrothermal process with 3-D ball milling can show enhanced electrochemical performance for the fabrication of an EDLC supercapacitor device.
Technological Insights on Glycerol Valorization into Propanediol through Thermocatalytic and Synthetic Biology Approaches
Authors: Yahyazadeh Arash , Bot Austin , Nanda Sonil , Dalai Ajay K.
Published: October 2023

The adverse effects of climate change, predominantly propelled by greenhouse gas emissions from fossil fuels, underscore the urgency of seeking sustainable alternatives to fossil fuel use. Amid growing concerns about climate change caused by fossil fuels and petrochemicals, this review focuses on sustainable solutions through the conversion of glycerol into value-added biochemicals. Glycerol, as the main byproduct of biodiesel production, is a particularly attractive chemical due to its potential to be upgraded into value-added building blocks and biochemicals. This review provides a detailed analysis of different thermochemical (catalytic) and synthetic biology (fermentative) pathways for the conversion of glycerol into 1,2-propanediol and 1,3-propanediol, which have proven industrial and commercial applications globally. The synthesis of propanediol from glycerol hydrogenolysis and other catalytic processes using different active metals and acidic oxides is reviewed. The reaction mechanism involved in hydrogenolysis reactions concerning the surface reaction mechanism is systematically discussed. The metabolic activities of promising microorganisms in fermenting glycerol, as the carbon source used to produce propanediol, are illustrated and elaborated. Combining these insights, this review is a comprehensive resource that can foster a better understanding of glycerol transformation into propanediol and its implications for sustainable chemistry and industrial practices. This exploration of alternative methods emphasizes the potential of sustainable approaches to reshape production practices and contribute to climate change mitigation.
Effect of Biobased Cling Films on Cheese Quality: Color and Aroma Analysis for Sustainable Food Packaging
Authors: Chirilli Chiara , Torri Luisa
Published: October 2023

Biobased and biodegradable polymeric materials are a sustainable alternative to the conventional plastics used in food packaging. This study investigated the possible effect of biobased cling films derived from renewable and circular and sustainable sources on key cheese sensory parameters (appearance and odor) able to influence consumer acceptance or rejection of a food product over time. For this purpose, a semi-hard cheese was selected as food model and stored for 14 days at 5 °C wrapped with five cling films: two bio-plastic materials from renewable circular and sustainable sources (R-BP1 and R-BP2), one bio-plastic film from a non-renewable source (NR-BP), and two conventional cling films (LDPE and PVC). Three analytical approaches (image analysis, electronic nose, and sensory test) were applied to evaluate the variation and the acceptability in terms of appearance and odor of the cheese. In preserving cheese color, the R-BP1 and RBP2 films were comparable to LDPE film, while NR-BP film was comparable to PVC film. In terms of odor preservation, R-BP2 film was comparable to LDPE and PVC. The consumer test showed that appearance and odor scores were higher for cheeses stored in R-BP1 and R-BP2 films than NR-BP film. Moreover, in terms of odor, R-BP1 film performed better than conventional films. This study shows how biodegradable cling films from renewable circular and sustainable resources could have comparable performance to conventional plastics (LDPE and PVC) used in the food sector.
Burden of Care of Family Caregivers for People Diagnosed with Serious Mental Disorders in a Rural Health District in Kwa-Zulu-Natal, South Africa
Authors: Ndlovu Jabulile Thembelihle , Mokwena Kebogile Elizabeth
Published: October 2023

An estimated 6% of the world population has serious mental illness, with one in four families having a member with some form of psychiatric disorder, who is mostly cared for by their relatives within a family setting. Although care-giving in a home setting is reported to be associated with significant mental distress, the burden of such distress is rarely measured. The purpose of this study was to quantify the burden of care among family caregivers of relatives with serious mental disorders, as well as to explore possible associations between the caregiver burden of care and a range of caregiver and Mental Health Care User (MHCU) variables in a rural district in Kwa-Zulu Natal, South Africa. The Zarit Burden Interview (ZBI) scale was used to collect data from 357 caregivers, and STATA 14 was used to analyze data. The ages of the sample ranged from 18 to 65 years, with a mean of 50.29, and the majority (86%) were female and unemployed (83%). The ZBI scores ranged from 8 to 85, with a mean of 41.59. The majority (91%) were found to be affected by family caregiver burden, which ranged from mild to severe. Using the Pearson Chi-square test of association (p = 0.05), variables that were significantly associated with the burden of care were clinically related (caregiver self-reported depression, MHCU diagnosis, recent relapse of the MHCU), socio-economic (caregiver family monthly income, MHCU disability grant status and MHCU employment status) and socio-demographic (MHCU gender and MHCU level of education). The prevalence of the burden of care is high and severe, and the scarcity of resources in families and communities contributes to the high burden of care in these rural communities.
A Soil Moisture Profile Conceptual Framework to Identify Water Availability and Recovery in Green Stormwater Infrastructure
Authors: Shakya Matina , Hess Amanda , Wadzuk Bridget M. , Traver Robert G.
Published: October 2023

The recovery of soil void space through infiltration and evapotranspiration processes within green stormwater infrastructure (GSI) is key to continued hydrologic function. As such, soil void space recovery must be well understood to improve the design and modeling and to provide realistic expectations of GSI performance. A novel conceptual framework of soil moisture behavior was developed to define the soil moisture availability at pre-, during, and post-storm conditions. It uses soil moisture measurements and provides seven critical soil moisture points (A, B, C, D, E, F, F″) that describe the soil–water void space recovery after a storm passes through a GSI. The framework outputs a quantification of a GSI subsurface hydrology, including average soil moisture, the duration of saturation, soil moisture recession, desaturation time, infiltration rates, and evapotranspiration (ET) rates. The outputs the framework provide were compared to the values that were obtained through more traditional measurements of infiltration (through spot field infiltration testing), ET (through a variety of methods to quantify GSI ET), soil moisture measurements (through the soil water characteristics curve), and the duration of saturation/desaturation time (through a simulated runoff test), all which provided a strong justification to the framework. This conceptual framework has several applications, including providing an understanding of a system’s ability to hold water, the post-storm recovery process, GSI unit processes (ET and infiltration), important water contents that define the soil–water relationship (such as field capacity and saturation), and a way to quantify long-term changes in performance all through minimal monitoring with one or more soil moisture sensors. The application of this framework to GSI design promotes a deeper understanding of the subsurface hydrology and site-specific soil conditions, which is a key advancement in the understanding of long-term performance and informing GSI design and maintenance.
Do Locations of Employment and Residence Influence whether People Use Virtual Social Networks? A Case Study of Residents in Wuhan, China
Authors: Deng Huixia , Niu Qiang , Wu Lei
Published: October 2023

High-speed information technology development has made virtual social networking (VSN) a social interaction trend. Studies have been carried out to investigate the spatial clustering characteristics of the locations where there is online social interaction, but they have not yet concentrated on the geographic phenomenon associated with the distribution of occupational and residential locations of citizens who use VSN. According to usage statistics gathered from China Unicom for people living in the Wuhan metropolitan development area, there are geographical characteristics for the sites of employment and residence of virtual social application (VSA) users. Compared with people who live or work in the central city, suburban citizens are more willing to conduct virtual social networking, and those who are most likely to do so are concentrated in the suburbs 20–30 km from the main city. Additionally, we used geographically weighted regressions to evaluate the relationship between the density of physical social facilities and the possibility of the usage of VSAs, revealing the influence of various conventional social conveniences on the propensity to use the VSA. Residents are more inclined to engage in VSN in places where traditional social interaction is inconvenient, particularly in suburbs, indicating that VSN is an addition to traditional social interaction. Nonetheless, neither an improvement in, nor the replacement of, VSN activities is apparent in places where conventional socializing is practical. This study identified the clustering of virtual social users’ places of employment and residence in metropolitan areas and concluded that virtual social interaction offers new social channels for people who lack access to adequate physical social facilities; that is, it complements traditional social interaction. These results can deepen the understanding of the relationship between traditional social interaction and VSN. They also offer a fresh viewpoint on facility planning for the potential future creation of a more balanced and diverse social interaction environment through the joint planning of virtual and physical social facilities.
Melatonin and Vitamins as Protectors against the Reproductive Toxicity of Bisphenols: Which Is the Most Effective? A Systematic Review and Meta-Analysis
Authors: Peña-Corona Sheila I. , Chávez-Corona Juan I. , Pérez-Caltzontzin Luis E. , Vargas-Estrada Dinorah , Mendoza-Rodríguez C. Adriana , Ramos-Martínez Edgar , Cerbón-Gutiérrez Jose L. , Herrera-Barragán José A. , Quintanar-Guerrero David , Leyva-Gómez Gerardo
Published: October 2023

Bisphenols such as bisphenol A (BPA), S (BPS), C (BPC), F (BPF), AF (BPAF), tetrabromobisphenol, nonylphenol, and octylphenol are plasticizers used worldwide to manufacture daily-use articles. Exposure to these compounds is related to many pathologies of public health importance, such as infertility. Using a protector compound against the reproductive toxicological effects of bisphenols is of scientific interest. Melatonin and vitamins have been tested, but the results are not conclusive. To this end, this systematic review and meta-analysis compared the response of reproductive variables to melatonin and vitamin administration as protectors against damage caused by bisphenols. We search for controlled studies of male rats exposed to bisphenols to induce alterations in reproduction, with at least one intervention group receiving melatonin or vitamins (B, C, or E). Also, molecular docking simulations were performed between the androgen (AR) and estrogen receptors (ER), melatonin, and vitamins. About 1234 records were initially found; finally, 13 studies were qualified for review and meta-analysis. Melatonin plus bisphenol improves sperm concentration and viability of sperm and increases testosterone serum levels compared with control groups; however, groups receiving vitamins plus bisphenols had lower sperm concentration, total testis weight, and testosterone serum levels than the control. In the docking analysis, vitamin E had the highest negative MolDock score, representing the best binding affinity with AR and ER, compared with other vitamins and melatonin in the docking. Our findings suggest that vitamins could act as an endocrine disruptor, and melatonin is most effective in protecting against the toxic effects of bisphenols.
Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells
Authors: Möller Jack K. S. , Linowiecka Kinga , Gagat Maciej , Brożyna Anna A. , Foksiński Marek , Wolnicka-Glubisz Agnieszka , Pyza Elżbieta , Reiter Russel J. , Tulic Meri K. , Slominski Andrzej T. , Steinbrink Kerstin , Kleszczyński Konrad
Published: October 2023

Melatonin (N-acetyl-5-methoxytryptamine, MEL), its kynurenic (N1-acetyl-N2-formyl-5-methoxykynurenine, AFMK) and indolic derivatives (6-hydroxymelatonin, 6(OH)MEL and 5-methoxytryptamine, 5-MT) are endogenously produced in human epidermis. Melatonin, produced by the pineal gland, brain and peripheral organs, displays a diversity of physiological functions including anti-inflammatory, immunomodulatory, and anti-tumor capacities. Herein, we assessed their regulatory effect on melanogenesis using amelanotic (A375, Sk-Mel-28) and highly pigmented (MNT-1, melanotic) human melanoma cell lines. We discovered that subjected compounds decrease the downstream pathway of melanin synthesis by causing a significant drop of cyclic adenosine monophosphate (cAMP) level, the microphthalmia-associated transcription factor (MITF) and resultant collapse of tyrosinase (TYR) activity, and melanin content comparatively to N-phenylthiourea (PTU, a positive control). We observed a reduction in pigment in melanosomes visualized by the transmission electron microscopy. Finally, we assessed the role of G-protein-coupled seven-transmembrane-domain receptors. Obtained results revealed that nonselective MT1 and MT2 receptor antagonist (luzindole) or selective MT2 receptor antagonist (4-P-PDOT) did not affect dysregulation of the melanin pathway indicating a receptor-independent mechanism. Our findings, together with the current state of the art, provide a convenient experimental model to study the complex relationship between metabolites of melatonin and the control of pigmentation serving as a future and rationale strategy for targeted therapies of melanoma-affected patients.
Diaphragm Fatigue in SMNΔ7 Mice and Its Molecular Determinants: An Underestimated Issue
Authors: Cadile Francesca , Recchia Deborah , Ansaldo Massimiliano , Rossi Paola , Rastelli Giorgia , Boncompagni Simona , Brocca Lorenza , Pellegrino Maria Antonietta , Canepari Monica
Published: October 2023

Spinal muscular atrophy (SMA) is a genetic disorder characterized by the loss of spinal motor neurons leading to muscle weakness and respiratory failure. Mitochondrial dysfunctions are found in the skeletal muscle of patients with SMA. For obvious ethical reasons, the diaphragm muscle is poorly studied, notwithstanding the very important role that respiratory involvement plays in SMA mortality. The main goal of this study was to investigate diaphragm functionality and the underlying molecular adaptations in SMNΔ7 mice, a mouse model that exhibits symptoms similar to that of patients with intermediate type II SMA. Functional, biochemical, and molecular analyses on isolated diaphragm were performed. The obtained results suggest the presence of an intrinsic energetic imbalance associated with mitochondrial dysfunction and a significant accumulation of reactive oxygen species (ROS). In turn, ROS accumulation can affect muscle fatigue, cause diaphragm wasting, and, in the long run, respiratory failure in SMNΔ7 mice. Exposure to the antioxidant molecule ergothioneine leads to the functional recovery of the diaphragm, confirming the presence of mitochondrial impairment and redox imbalance. These findings suggest the possibility of carrying out a dietary supplementation in SMNΔ7 mice to preserve their diaphragm function and increase their lifespan.
Management of Open Pediatric Fractures: Proposal of a New Multidisciplinary Algorithm
Authors: Aulisa Angelo Gabriele , Marsiolo Martina , Basiglini Luca , Aletto Cristian , Giordano Marco , Falciglia Francesco
Published: October 2023

Background: An algorithm for managing open fractures in children is still being debated; the present study suggests an evidence-based way to manage these patients in the emergency department. Methods: The literature on “Open fractures in children” was carefully analyzed using keywords. The primary sources were The Cochrane Library, PubMed, and Researchgate. Conclusion: We proposed an evidence-based algorithm for managing open fractures in children to standardize clinical practice and improve the care of these patients.
Predictions of Wave Overtopping Using Deep Learning Neural Networks
Authors: Tsai Yu-Ting , Tsai Ching-Piao
Published: October 2023

Deep learning techniques have revolutionized the field of artificial intelligence by enabling accurate predictions of complex natural scenarios. This paper proposes a novel convolutional neural network (CNN) model that involves deep learning technologies, such as the bottleneck residual block, layer normalization, and dropout layer, to predict wave overtopping at coastal structures under a wide range of conditions. To optimize the performance of the CNN model, the hyperparameter tuning process via Bayesian optimization is used. The results of validation demonstrate that the proposed CNN model is highly accurate in estimating wave overtopping discharge from hydraulic and structural parameters. The testing accuracy of the overtopping predictions using a prototype dataset shows that the proposed CNN model outperforms those existing machine learning models. An example application of the CNN model is presented for predicting prototype overtopping considering various crest freeboards of coastal structures.
A Comprehensive Review on Material Compatibility and Safety Standards for Liquid Hydrogen Cargo and Fuel Containment Systems in Marine Applications
Authors: Kim Myung-Sung , Chun Kang Woo
Published: October 2023

As the maritime industry’s emphasis on sustainable fuels has increased, liquid hydrogen (LH2) has emerged as a promising alternative due to its high energy density and zero-emission characteristics. While the experience of using natural gas in ships can serve as a basis for the introduction of hydrogen, the different risks associated with the two fuels must also be considered. This review article provides a methodology for selecting suitable metal materials for shipboard LH2 storage and piping systems based on operational requirements. The effects of both liquid and gaseous hydrogen environments on metal materials are first comprehensively reviewed. The minimum requirements for metal materials in liquefied natural gas (LNG) storage systems, as stipulated in the IGC and IGF codes, were used as a baseline to establish minimum requirements for liquid hydrogen. The applicability of austenitic stainless steel, a representative metal material for cryogenic use, to a liquid hydrogen environment according to nickel content was examined. In order to apply liquid hydrogen to the marine environment, the minimum requirements for liquid hydrogen were organized based on the minimum requirements for metal materials in the LNG storage system covered by the IGC and IGF codes. Finally, to expand the material selection criteria for low-temperature cargo and fuel storage facilities at sea, slow strain tensile testing, fatigue life, and fracture toughness considering the hydrogen environment and cryogenic temperature were derived as evaluation items.