bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishment of a Lung Cancer Discriminative Model Based on an Optimized Support Vector Machine Algorithm and Study of Key Targets of Wogonin in Lung Cancer
Authors: Wang Lin , Zhang Jianhua , Shan Guoyong , Liang Junting , Jin Wenwen , Li Yingyue , Su Fangchu , Ba Yanhua , Tian Xifeng , Sun Xiaoyan , Zhang Dayong , Zhang Weihua , Chen Chuan liang
Published: September 2021

An optimized support vector machine model was used to construct a lung cancer diagnosis model based on serological indicators, and a molecular regulation model of Wogonin, a component of Scutellaria baicalensis, was established. Serological indexes of patients were collected, the grid search method was used to identify the optimal penalty coefficient C and parameter g of the support vector machine model, and the benign and malignant auxiliary diagnosis model of isolated pulmonary nodules based on serological indicators was established. The regulatory network and key targets of Wogonin in lung cancer were analyzed by network pharmacology, and key targets were detected by western blot. The relationship between serological susceptibility genes and key targets of Wogonin was established, and the signaling pathway of Wogonin regulating lung cancer was constructed. After support vector machine parameter optimization (C = 90.597, g = 32), the accuracy of the model was 90.8333%, with nine false positives and two false negative cases. Ontology functional analysis of 67 common genes between Wogonin targets and lung cancer–related genes showed that the targets were associated with biological processes involved in peptidye-serine modification and regulation of protein kinase B signaling; cell components in the membrane raft and chromosomal region; and molecular function in protein serine/threonine kinase activity and heme binding. Kyoto Encyclopedia of Genes and Genomes analysis showed that the regulation pathways involved the PI3K-Akt signaling pathway, ERBB signaling pathway, and EGFR tyrosine kinase inhibitor resistance. In vitro analyses using lung cancer cells showed that Wogonin led to significantly increased levels of cleaved caspase-3 and Bad and significantly decreased Bcl-2 expression in a concentration-dependent manner. ErbB4 expression also significantly decreased in lung cancer cells after treatment with Wogonin. A regulatory network of Wogonin regulating lung cancer cell apoptosis was constructed, including the participation of serological susceptibility genes. There is a certain regulatory effect between the serological indexes that can be used in the diagnosis of lung cancer and the key targets of Chinese herbal medicine treatment of lung cancer, which provides a new idea for the diagnosis, treatment and prognosis of clinical lung cancer.
Studies on Activities and Chemical Characterization of Medicinal Plants in Search for New Antimalarials: A Ten Year Review on Ethnopharmacology
Authors: Ceravolo Isabela P. , Aguiar Anna C. , Adebayo Joseph O. , Krettli Antoniana U.
Published: September 2021

Malaria is an endemic disease that affected 229 million people and caused 409 thousand deaths, in 2019. Disease control is based on early diagnosis and specific treatment with antimalarial drugs since no effective vaccines are commercially available to prevent the disease. Drug chemotherapy has a strong historical link to the use of traditional plant infusions and other natural products in various cultures. The research based on such knowledge has yielded two drugs in medicine: the alkaloid quinine from Cinchona species, native in the Amazon highland rain forest in South America, and artemisinin from Artemisia annua, a species from the millenary Chinese medicine. The artemisinin-based combination therapies (ACTs), proven to be highly effective against malaria parasites, and considered as “the last bullet to fight drug-resistant malaria parasites,” have limited use now due to the emergence of multidrug resistance. In addition, the limited number of therapeutic options makes urgent the development of new antimalarial drugs. This review focuses on the antimalarial activities of 90 plant species obtained from a search using Pubmed database with keywords “antimalarials,” “plants” and “natural products.” We selected only papers published in the last 10 years (2011–2020), with a further analysis of those which were tested experimentally in malaria infected mice. Most plant species studied were from the African continent, followed by Asia and South America; their antimalarial activities were evaluated against asexual blood parasites, and only one species was evaluated for transmission blocking activity. Only a few compounds isolated from these plants were active and had their mechanisms of action delineated, thereby limiting the contribution of these medicinal plants as sources of novel antimalarial pharmacophores, which are highly necessary for the development of effective drugs. Nevertheless, the search for bioactive compounds remains as a promising strategy for the development of new antimalarials and the validation of traditional treatments against malaria. One species native in South America, Ampelozyzyphus amazonicus, and is largely used against human malaria in Brazil has a prophylactic effect, interfering with the viability of sporozoites in in vitro and in vivo experiments.
Tempol Reverses the Negative Effects of Morphine on Arterial Blood-Gas Chemistry and Tissue Oxygen Saturation in Freely-Moving Rats
Authors: Baby Santhosh M. , Discala Joseph F. , Gruber Ryan , Getsy Paulina M. , Cheng Feixiong , Damron Derek S. , Lewis Stephen J.
Published: September 2021

We have reported that pretreatment with the clinically approved superoxide dismutase mimetic, Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), blunts the cardiorespiratory depressant responses elicited by a subsequent injection of fentanyl, in halothane-anesthetized rats. The objective of the present study was to determine whether Tempol is able to reverse the effects of morphine on arterial blood-gas (ABG) chemistry in freely-moving Sprague Dawley rats. The intravenous injection of morphine (10 mg/kg) elicited substantial decreases in pH, pO2 and sO2 that were accompanied by substantial increases in pCO2 and Alveolar-arterial gradient, which results in diminished gas-exchange within the lungs. Intravenous injection of a 60 mg/kg dose of Tempol 15 min after the injection of morphine caused minor improvements in pO2 and pCO2 but not in other ABG parameters. In contrast, the 100 mg/kg dose of Tempol caused an immediate and sustained reversal of the negative effects of morphine on arterial blood pH, pCO2, pO2, sO2 and Alveolar-arterial gradient. In other rats, we used pulse oximetry to determine that the 100 mg/kg dose of Tempol, but not the 60 mg/kg dose elicited a rapid and sustained reversal of the negative effects of morphine (10 mg/kg, IV) on tissue O2 saturation (SpO2). The injection of morphine caused a relatively minor fall in mean arterial blood pressure that was somewhat exacerbated by Tempol. These findings demonstrate that Tempol can reverse the negative effects of morphine on ABG chemistry in freely-moving rats paving the way of structure-activity and mechanisms of action studies with the host of Tempol analogues that are commercially available.
Circulating MicroRNAs in the Second Trimester From Pregnant Women Who Subsequently Developed Preeclampsia: Potential Candidates as Predictive Biomarkers and Pathway Analysis for Target Genes of miR-204-5p
Authors: Luizon Marcelo R. , Conceição Izabela M. C. A. , Viana-Mattioli Sarah , Caldeira-Dias Mayara , Cavalli Ricardo C. , Sandrim Valeria C.
Published: September 2021

MicroRNAs (miRNAs) play an important role in the pathophysiology of preeclampsia (PE). However, the expression of circulating miRNAs was not analyzed in the second trimester of pregnancy, a period of major relevance to identify predictive biomarkers for PE. Therefore, we examined the expression profiles of 84 circulating miRNAs using a PCR array in plasma collected between 20 and 25 weeks of gestation from pregnant women, who subsequently developed PE and those who remained healthy during pregnancy, randomly selected from a prospective cohort. Overall, 23 miRNAs had a fold change > 2.0 and were considered to be upregulated in plasma from pregnant women who subsequently developed PE, even before the onset of clinical symptoms of PE. However, only miR-204-5p was statistically significant (P = 0.0082). Experimentally validated interactions for the target genes of miR-204-5p extracted from miRTarBase were used in the gene set functional enrichment analysis to identify Reactome pathways. The network connecting the 37 target genes for miR-204-5p revealed pathways of known pathophysiological relevance during the early development of PE and included key genes related to PE, such as BDNF, MMP-9, MALAT1, TGFBR2, and SIRT1. We further depicted downstream targets of SIRT1 that are related to the vascular endothelial function or implicated in the pathophysiology of PE, namely, FOXO1, NFκB, HIF-1α, NOS3, and PPAR-γ. Our novel findings provide for circulating miRNAs upregulated in the second trimester on plasma from pregnant women who subsequently developed PE that is potentially related to the early development of PE, which may guide further studies focused on the validation of potential predictive biomarkers in PE.
POD-Enhanced Deep Learning-Based Reduced Order Models for the Real-Time Simulation of Cardiac Electrophysiology in the Left Atrium
Authors: Fresca Stefania , Manzoni Andrea , Dedè Luca , Quarteroni Alfio
Published: September 2021

The numerical simulation of multiple scenarios easily becomes computationally prohibitive for cardiac electrophysiology (EP) problems if relying on usual high-fidelity, full order models (FOMs). Likewise, the use of traditional reduced order models (ROMs) for parametrized PDEs to speed up the solution of the aforementioned problems can be problematic. This is primarily due to the strong variability characterizing the solution set and to the nonlinear nature of the input-output maps that we intend to reconstruct numerically. To enhance ROM efficiency, we proposed a new generation of non-intrusive, nonlinear ROMs, based on deep learning (DL) algorithms, such as convolutional, feedforward, and autoencoder neural networks. In the proposed DL-ROM, both the nonlinear solution manifold and the nonlinear reduced dynamics used to model the system evolution on that manifold can be learnt in a non-intrusive way thanks to DL algorithms trained on a set of FOM snapshots. DL-ROMs were shown to be able to accurately capture complex front propagation processes, both in physiological and pathological cardiac EP, very rapidly once neural networks were trained, however, at the expense of huge training costs. In this study, we show that performing a prior dimensionality reduction on FOM snapshots through randomized proper orthogonal decomposition (POD) enables to speed up training times and to decrease networks complexity. Accuracy and efficiency of this strategy, which we refer to as POD-DL-ROM, are assessed in the context of cardiac EP on an idealized left atrium (LA) geometry and considering snapshots arising from a NURBS (non-uniform rational B-splines)-based isogeometric analysis (IGA) discretization. Once the ROMs have been trained, POD-DL-ROMs can efficiently solve both physiological and pathological cardiac EP problems, for any new scenario, in real-time, even in extremely challenging contexts such as those featuring circuit re-entries, that are among the factors triggering cardiac arrhythmias.
Protective Role of Vitamin B1 in Doxorubicin-Induced Cardiotoxicity in Rats: Focus on Hemodynamic, Redox, and Apoptotic Markers in Heart
Authors: Rankovic Marina , Draginic Nevena , Jeremic Jovana , Samanovic Andjela Milojevic , Stojkov Svetlana , Mitrovic Slobodanka , Jeremic Nevena , Radonjic Tanja , Srejovic Ivan , Bolevich Sergey , Svistunov Andrey , Jakovljevic Vladimir , Turnic Tamara Nikolic
Published: September 2021

Up until now, the specific mechanisms involved in doxorubicin (DOX)-induced cardiotoxicity have not been fully elucidated. Since thiamine deficiency is associated with myocardial dysfunction and it may lead to cardiomyopathy, we aimed to investigate whether thiamine (Vitamin B1) treatment provides cardioprotection and modulates DOX mediated subchronic cardiotoxicity as well as to determine possible mechanisms of its effects. The study involved 48 Wistar albino rats divided into four groups: healthy non-treated rats and healthy rats treated with thiamine and DOX rats without treatment and DOX rats treated with thiamine. DOX was applied as a single i.p.injection (15mg/kg), while thiamine treatment lasted 7days (25mg/kg/dayi.p.). Before and after the treatment hemodynamic changes were monitoredin vivoby echocardiography. When the protocol was completed, animals were sacrificed and rat hearts were isolated in order to evaluate parameters of cardiac oxidative stress [superoxide anion radical-O2−, hydrogen peroxide-H2O2, nitric oxide-NO−, index of lipid peroxidation-thiobarbituric acid (TBA) reactive substances (TBARS), superoxide dismutase – SOD, catalase (CAT), and reduced glutathione-GSH] and apoptosis (Bax, Bcl-2, caspases). DOX treatment significantly reduced the ejection fraction, while thiamine treatment led to its minor increase in the DOX-treated group. In that sense, heart oxidative stress markers were significantly increased in DOX-treated rats, while therapeutic dose of thiamine decreased the levels of free radicals. Our study demonstrated the promising ameliorative effects of thiamine against DOX-induced cardiotoxicity through modulation of oxidative stress, suppression of apoptosis, and possibility to improve myocardial performance and morphometric structure of rats` hearts.
Acceptability of Artificial Intelligence in Poultry Processing and Classification Efficiencies of Different Classification Models in the Categorisation of Breast Fillet Myopathies
Authors: Siddique Aftab , Shirzaei Samira , Smith Alice E. , Valenta Jaroslav , Garner Laura J. , Morey Amit
Published: September 2021

Breast meat from modern fast-growing big birds is affected with myopathies such as woody breast (WB), white striping, and spaghetti meat (SM). The detection and separation of the myopathy-affected meat can be carried out at processing plants using technologies such as bioelectrical impedance analysis (BIA). However, BIA raw data from myopathy-affected breast meat are extremely complicated, especially because of the overlap of these myopathies in individual breast fillets and the human error associated with the assignment of fillet categories. Previous research has shown that traditional statistical techniques such as ANOVA and regression, among others, are insufficient in categorising fillets affected with myopathies by BIA. Therefore, more complex data analysis tools can be used, such as support vector machines (SVMs) and backpropagation neural networks (BPNNs), to classify raw poultry breast myopathies using their BIA patterns, such that the technology can be beneficial for the poultry industry in detecting myopathies. Freshly deboned (3–3.5 h post slaughter) breast fillets (n = 100 × 3 flocks) were analysed by hand palpation for WB (0-normal; 1-mild; 2-moderate; 3-Severe) and SM (presence and absence) categorisation. BIA data (resistance and reactance) were collected on each breast fillet; the algorithm of the equipment calculated protein and fat index. The data were analysed by linear discriminant analysis (LDA), and with SVM and BPNN with 70::30: training::test data set. Compared with the LDA analysis, SVM separated WB with a higher accuracy of 71.04% for normal (data for normal and mild merged), 59.99% for moderate, and 81.48% for severe WB. Compared with SVM, the BPNN training model accurately (100%) separated normal WB fillets with and without SM, demonstrating the ability of BIA to detect SM. Supervised learning algorithms, such as SVM and BPNN, can be combined with BIA and successfully implemented in poultry processing to detect breast fillet myopathies.
Current Advancements and Limitations of Gene Editing in Orphan Crops
Authors: Venezia Matthew , Creasey Krainer Kate M.
Published: September 2021

Gene editing provides precise, heritable genome mutagenesis without permanent transgenesis, and has been widely demonstrated and applied in planta. In the past decade, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) has revolutionized the application of gene editing in crops, with mechanistic advances expanding its potential, including prime editing and base editing. To date, CRISPR/Cas has been utilized in over a dozen orphan crops with diverse genetic backgrounds, leading to novel alleles and beneficial phenotypes for breeders, growers, and consumers. In conjunction with the adoption of science-based regulatory practices, there is potential for CRISPR/Cas-mediated gene editing in orphan crop improvement programs to solve a plethora of agricultural problems, especially impacting developing countries. Genome sequencing has progressed, becoming more affordable and applicable to orphan crops. Open-access resources allow for target gene identification and guide RNA (gRNA) design and evaluation, with modular cloning systems and enzyme screening methods providing experimental feasibility. While the genomic and mechanistic limitations are being overcome, crop transformation and regeneration continue to be the bottleneck for gene editing applications. International collaboration between all stakeholders involved in crop improvement is vital to provide equitable access and bridge the scientific gap between the world’s most economically important crops and the most under-researched crops. This review describes the mechanisms and workflow of CRISPR/Cas in planta and addresses the challenges, current applications, and future prospects in orphan crops.
Public and Stakeholder Engagement in Developing Human Heritable Genome Editing Policies: What Does it Mean and What Should it Mean?
Authors: Iltis Ana S. , Hoover Sarah , Matthews Kirstin R. W.
Published: September 2021

As scientific research pushes the boundaries of knowledge, new discoveries and technologies often raise ethical and social questions. Public responses vary from surprise, to unrealistic optimism about imminent new treatments, confusion, and absolute opposition. Regardless of the intent, the use of a precise gene editing tool on human embryos, such as CRISPR-Cas9, is an example of such a controversial emerging technology. Substantive disagreement about the appropriate research pathways and permissible clinical applications is to be expected. Many ethical concerns, especially related to genetic manipulation of human embryos, are rooted in deeply held moral, religious, or ideological beliefs that science alone cannot address. Today, more scientists and scientific societies as well as policy makers are calling for public and stakeholder engagement in developing guidelines and policies governing scientific practice. We conducted a critical interpretive review of the literature on public and stakeholder engagement in science policy development regarding emerging technologies to determine the ideals that should guide engagement efforts of entities developing recommendations or guidelines on policy for such technologies. We identify and describe five ideals. To illustrate possible applications of these ideals, we review the engagement efforts described in three reports on heritable human genome editing and assess those efforts in light of these ideals. Finally, we recommend possible avenues for engagement that would advance those goals.
Levels of Conceptualization and Municipal Politics: Replication in a New Context
Authors: Matthews J. Scott , McGregor R. Michael , Stephenson Laura B.
Published: September 2021

Since Angus Campbell and colleagues introduced the Levels of Conceptualization (LoC) framework as a measure of political sophistication, only a very small number of scholars have applied this approach to understanding how electors view political actors. In 2008, Michael Lewis-Beck and colleagues replicated this foundational study and found similar results using much more recent data on American national elections. In this brief research report, we replicate the work of Lewis-Beck and colleagues in the Canadian municipal context. Using survey data from the Canadian Municipal Election Study, we make use of open-ended responses about attitudes towards mayoral candidates to conduct a qualitative examination of the manner in which survey respondents from eight Canadian cities view mayoral candidates. Despite the relative dearth of ideological cues at the local level, we nevertheless find that a noteworthy portion of the electorate views candidates in ideological terms. Like previous work on the subject, we find that high levels of conceptualization are positively associated with turnout, education, political knowledge, and ‘political involvement’.
The Factor Structure and Rasch Analysis of the Fear of COVID-19 Scale (FCV-19S) Among Chinese Students
Authors: Chen Wei , Liang Yuxin , Yin Xingyu , Zhou Xingrong , Gao Rongfen
Published: September 2021

The Fear of COVID-19 Scale (FCV-19S) is a new one-dimensional scale used to measure fear of an individual about the COVID-19. Given the seriousness of the COVID-19 situation in China when our study was taking place, our aim was to translate and examine the applicability of the FCV-19S in Chinese students. The sample used for validation comprised 2,445 Chinese students. The psychometrical characteristics of the Chinese FCV-19S (FCV-19S-C) were tested using Rasch analysis. Principal component analysis (PCA) proved the unidimensional structure of the model. Both infit and outfit mean square (MNSQ) values (0.69–1.31) and point-measure correlations (0.82–0.86) indicated a good model fit. Person-item separation and reliability values indicated good reliability of the scale. The person-item map revealed an acceptable level of match between the persons and the items. Differential item functioning of the FCV-19S-C showed no differences with respect to age or gender. FCV-19S-C scores were significantly associated with anxiety, stress, depression, ego-resilience, and general health. The FCV-19S-C was proven to be effective in measuring fear of Chinese students about the COVID-19.
The Emergence of Lying for Reputational Concerns in 5-Year-Olds
Authors: Klafka Mareike , Liszkowski Ulf
Published: September 2021

Research suggests that even young children engage in strategic behaviors to manipulate the impressions others form of them and that they manage their reputation in order to cooperate with others. The current study investigated whether young children also lie in order to manage their, or their group’s, reputation in front of ingroup and outgroup members. Five-year old children (n=55) were randomly assigned to an individual reputation condition or a group reputation condition. Then, they played a mini dictator game in which they could share privately any number of their or their group’s stickers with an anonymous child. Participants then met ingroup and outgroup members, established through a minimal group design, via a pre-recorded, staged Skype call. Group members asked the participant how many stickers she, or her group, had donated. Results revealed that children stated to peers to have donated more than their actual donation, with no differences between conditions and no difference toward ingroup and outgroup members. Findings suggest that by 5years of age, children use lying as a strategy to manage their reputation.
Dutch Forensic Flexible Assertive Community Treatment: Operating on the Interface Between General Mental Health Care and Forensic Psychiatric Care
Authors: Smeekens Marjam V. , Sappelli Fedde , de Vries Meike G. , Bulten Berend H.
Published: September 2021

In the Netherlands, Forensic Flexible Assertive Community Treatment (ForFACT) is used as a specialized form of outpatient intensive treatment. This outreaching type of treatment is aimed at patients with severe and long lasting psychiatric problems that are at risk of engaging in criminal behavior. In addition, these patients often suffer from addiction and experience problems in different areas of their life (e.g., financial debt, unemployment, or lack of daytime activities). The aim of this exploratory study was to gain more insight into the characteristics of the ForFACT patient population. More knowledge about these patients may enhance the effectiveness of ForFACT and therefore (further) reduce the risk of recidivism. Data on 132 ForFACT patients were gathered by studying electronic patient records, criminal records, and by conducting semi-structured interviews with practitioners and patients. Additionally, as part of a cognitive screening, two screening instruments were conducted to gain insight into intelligence and possible mild cognitive impairments. This article gives a broad description of the ForFACT patient population, including demographic data and context variables, diagnostics, recidivism risk and offense history, and aspects related to care. Furthermore, several recommendations are given to further improve ForFACT. Based on the results it can be concluded that the ForFACT patient population shows a high degree of diversity in complex care needs and responsivity issues. Therefore, this article highlights the necessity for ForFACT to collaborate with other mental health institutions, as well as probation officers, and forensic or criminal justice institutions. Moreover, it is important to continually check the inclusion and exclusion criteria when admitting patients to ForFACT, and to examine whether ForFACT is still the most adequate care for patients or if they need to be referred. In addition, the results emphasize the importance of cognitive screening for forensic outpatients. Finally, this study zooms in on the interface between forensic psychiatric care and general mental health care.
Psychometric Validation of a Questionnaire to Assess Perception and Knowledge About Exposure to Pesticides in Rural Schoolchildren of Maule, Chile
Authors: Muñoz-Quezada María Teresa , Lucero Boris , Castillo Benjamín , Bradman Asa , Zúñiga Liliana , Baumert Brittney O. , Iglesias Verónica , Muñoz María Pía , Buralli Rafael J. , Antini Carmen
Published: September 2021

Exposure to pesticides during infancy is associated with numerous adverse health outcomes. The assessment of knowledge and perception of pesticides exposure and risk among children has not been thoroughly studied. The aim of the study was to evaluate the reliability and validity of a questionnaire that measures the knowledge and perception of exposure to organophosphate pesticides among rural schoolchildren. The questionnaire was administered to 151 schoolchildren between 9 and 13years from four Chilean rural schools. An internal consistency analysis of the ordinal alpha coefficient and a polychoric factor analysis for categorical data were used. The results show that the ordinal alpha was 0.95. Polychoric matrices of rotated components show the 17 questions summarized pesticide knowledge in five factors extracted after promax rotation. This factorial model explains 56.3% of the variance. The questions were grouped as follows: knowledge about pesticides (Factor 1); knowledge of health effects related to pesticides exposure (Factor 2); pesticide exposure through the growing of fruits and vegetables (Factor 3); perception and action against pesticides exposure at school (Factor 4); and perception and action against pesticides exposure at home (Factor 5). The questionnaire provides a useful tool for examining pesticide exposure in agricultural regions, allowing younger community members to participate.
Greater Social Interest Between Autistic and Non-autistic Conversation Partners Following Autism Acceptance Training for Non-autistic People
Authors: Jones Desiree R. , Morrison Kerrianne E. , DeBrabander Kilee M. , Ackerman Robert A. , Pinkham Amy E. , Sasson Noah J.
Published: September 2021

Bi-directional differences in social communication and behavior can contribute to poor interactions between autistic and non-autistic (NA) people, which in turn may reduce social opportunities for autistic adults and contribute to poor outcomes. Historically, interventions to improve social interaction in autism have focused on altering the behaviors of autistic people and have ignored the role of NA people. Recent efforts to improve autism understanding among NA adults via training have resulted in more favorable views toward autistic people, yet it remains unknown whether these benefits extend to real-world interactions between autistic and NA people. The current study explores whether a brief autism acceptance training (AAT) program can improve social interactions between autistic and NA adults. Thirty-nine NA males were randomly assigned to complete AAT or a no-training control condition, then participated in a 5-min unstructured conversation with an unfamiliar autistic male (n = 39). Following the conversation, participants rated their perceptions of interaction quality, first impressions of their partner, and their interest in future interactions with their partner. In dyads where the NA individual completed AAT, both the autistic and NA person endorsed greater future interest in hanging out with their partner relative to dyads in which the NA adult did not complete AAT. However, other social interaction outcomes, including ratings of interaction quality and first impressions of autistic partners, largely did not differ between training and no-training conditions, and assessments of the interaction were largely unrelated for autistic and NA partners within dyads. Results also indicated that NA participants, but not autistic participants, demonstrated substantial correspondence between evaluations of their partner and the interaction, suggesting that autistic adults may place less weight on trait judgments when assessing the quality of an interaction. These findings suggest that the brief AAT for NA adults used in this study may increase mutual social interest in real-world interactions between NA and autistic adults, but more systematic changes are likely needed to bridge divides between these individuals. Future work with larger, more diverse samples is recommended to further explore whether interventions targeting NA adults are beneficial for improving autistic experiences within NA social environments.
Autistic People's Access to Bilingualism and Additional Language Learning: Identifying the Barriers and Facilitators for Equal Opportunities
Authors: Davis Rachael , Fletcher-Watson Sue , Digard Bérengère G.
Published: September 2021

Bilingualism is a valuable tool that enriches and facilitates cultural, social and lived experiences for autistic and non-autistic people alike. Research consistently finds no negative effects of bilingualism and highlights the potential for positive effects across cognitive and socio-cultural domains for autistic and non-autistic children. Yet parents of autistic children remain concerned that bilingualism will cause delays in both cognitive and language development and are still frequently advised by practitioners to raise their child monolingually. Evidently, findings from research are not reflected in practice or subsequent advice, and it is essential to identify ways to ensure equal access to additional language learning. We briefly summarise the existing literature on bilingualism and autism, considering perspectives from the bilingual autistic community, and experimental research. We identify the most pertinent barriers to participation for autistic bilingual children in terms of familial, clinical and educational perspectives. We propose novel solutions to promote additional language learning and suggest changes to practice that will contribute to an evidence base for families and practitioners. This commentary makes innovative recommendations at both the individual and societal level to ensure that autistic bilingual people have equal rights and opportunities to language learning and are optimally supported in accessing them.
The Interaction Between Typically Developing Students and Peers With Autism Spectrum Disorder in Regular Schools in Ghana: An Exploration Using the Theory of Planned Behaviour
Authors: Opoku Maxwell Peprah , Nketsia William , J-F  , Mprah Wisdom Kwadwo , Agyei-Okyere Elvis , Safi Mohammed
Published: September 2021

The purpose of this study is to assess the intention of typically developing peers towards learning in the classroom with students with Autism Spectrum Disorder (ASD). In developing countries, such as Ghana, the body of literature on the relationship between students with disabilities and typically developing peers has been sparsely studied. Using Ajzen's theory of planned behaviour as a theoretical framework for this study, 516 typically developing students completed four scales representing belief constructs, attitudes, subjective norms, and perceived behavioural controls (self-efficacy), hypothesised to predict behavioural intention. The data were subjected to a t-test, analysis of variance, and structural equation modelling. The modelling confirmed the combining ability of attitude, subjective norms, and perceived behavioural controls to predict intention. We conclude by revealing the need for policymakers to consider designing programmes aimed towards promoting social relationships between students with ASD and typically developing peers.
Implications of Inflammatory States on Dysfunctional Immune Responses in Aging and Obesity
Authors: Thomas Alyssa L. , Alarcon Pablo C. , Divanovic Senad , Chougnet Claire A. , Hildeman David A. , Moreno-Fernandez Maria E.
Published: September 2021

Aging and obesity are two conditions characterized by chronic, low-grade inflammation. While both conditions are also associated with dysfunctional immune responses, the shared and distinct underlying mechanisms are just starting to be uncovered. In fact, recent findings have suggested that the effects of obesity on the immune system can be thought of as a state of accelerated aging. Here we propose that chronic, low-grade inflammation seen in obesity and aging is complex, affects multiple cell types, and results in an altered basal immune state. In aging, part of this altered state is the emergence of regulatory immune populations that lead to further immune dysfunction in an attempt to reduce chronic inflammation. While in obesity, part of the altered state is the effect of expanding adipose tissue on immune cell function. Thus, in this review, we compare, and contrast altered immune states in aging and obesity and discuss their potential contribution to a shared clinical problem- decreased vaccine responsiveness.
Oocyte Cryopreservation at a Young Age Provides an Effective Strategy for Expanding Fertile Lifespan
Authors: Poli Maurizio , Capalbo Antonio
Published: September 2021

With an upward trend in delaying parenthood, women across the world face an increasing risk of age-related infertility and involuntary childlessness. Elective oocyte banking strategies offer women the possibility to protect part of their reproductive potential until personal finances, personal relationship, or career have stabilized. Timely collection and cryopreservation of oocytes when they are most competent and chromosomal abnormality rates have not yet escalated are crucial for achieving high live births throughin vitrofertilization (IVF) treatment at a later stage. To promote reproductive autonomy, women shall be informed about the decrease in fertility rates that sharply intensifies from the age of 35 years and the strategies available to maintain their reproductive potential. Together with this information, women should also recognize the limitations of available strategies including expected live birth rates, costs of the procedures, and overall approach performance, which is mainly associated with age at cryopreservation, number of oocytes banked, and age at accessing the banked oocytes. Evidence-based statistics are not yet available due to the relatively short period in which oocyte cryopreservation has been offered for elective purposes and the scarce number of patients returning for accessing their oocytes. However, to evaluate the applicability of fertility cryopreservation on a large scale, several theoretical models have been proposed to assess the expected efficacy and overall cost-effectiveness of different oocyte banking strategies. In this study, we review current oocyte cryopreservation methodologies, their applications, and outcomes. Moreover, we summarize current evidence regarding known parameters affecting oocyte banking efficacy. Finally, we discuss key points that could play a role in improving access to the service and optimization of oocyte banking frameworks.
Asymmetry and Variability in the Transmission Spectra of Tidally Locked Habitable Planets
Authors: Song Xinyi , Yang Jun
Published: September 2021

Spatial heterogeneity and temporal variability are general features in planetary weather and climate, due to the effects of planetary rotation, uneven stellar flux distribution, fluid motion instability, etc. In this study, we investigate the asymmetry and variability in the transmission spectra of 1:1 spin–orbit tidally locked (or called synchronously rotating) planets around low-mass stars. We find that for rapidly rotating planets, the transit atmospheric thickness of the evening terminator (east of the substellar region) is significantly larger than that of the morning terminator (west of the substellar region). The asymmetry is mainly related to the spatial heterogeneity in ice clouds, as the contributions of liquid clouds and water vapor are smaller. The underlying mechanism is that there are always more ice clouds on the evening terminator, due to the combined effect of coupled Rossby–Kelvin waves and equatorial superrotation that advect vapor and clouds to the east, especially at high levels of the atmosphere. For slowly rotating planets, the asymmetry reverses (the morning terminator has a larger transmission depth than the evening terminator), but the magnitude is small or even negligible. For both rapidly and slowly rotating planets, there is strong variability in the transmission spectra. The asymmetry signal is nearly impossible to be observed by the James Webb Space Telescope (JWST), because the magnitude of the asymmetry (about 10 ppm) is smaller than the instrumental noise and the high variability further increases the challenge.
Dayside Diffuse Aurora and the Cold-Plasma Structuring: A Brief Review
Authors: Han De-Sheng
Published: September 2021

Diffuse aurora is generated by the precipitation of hot electrons from the central plasma sheet due to wave-particle interaction. Near magnetic local noon (MLN), the diffuse aurora was often observed in structured forms, such as in stripy or patchy. In the magnetosphere, when the hot electrons meet with a cold plasma structure, the threshold of resonance energy for the electrons in the cold plasma region can be lowered, leading to more electrons being involved in the wave-particle interaction and being scattered into the loss cone. As a result, stronger diffuse aurora can be produced in the correspondent region. Based on this mechanism, the structured dayside diffuse auroras have been suggested to correspond to the cold plasma structures in the dayside outer magnetosphere. This brief review focuses on showing that 1) the stripy diffuse auroras observed near MLN are specifically informative, 2) there are two types of diffuse aurora near MLN, which may correspond to cold plasmas originating from inside and outside the magnetosphere, respectively, and 3) we can study the inside-outside coupling by using the interaction between diffuse and discrete auroras observed near MLN.
Feasibility and Short-Term Outcomes of One-Step and Two-Step Sleeve Gastrectomy as Revision Procedures for Failed Adjustable Gastric Banding Compared With Those After Primary Sleeve Gastrectomy
Authors: Thaher Omar , Driouch Jamal , Hukauf Martin , Köckerling Ferdinand , Stroh Christine
Published: September 2021

Background: The practice of bariatric surgery was studied using the German Bariatric Surgery Registry (GBSR). The focus of the study was to evaluate whether revision surgery One-Step (OS) or Two-Step (TS) sleeve gastrectomy (SG) has a large benefit in terms of perioperative risk in patients after failed Adjustable Gastric Banding (AGB).

Methods: The data collection includes patients who underwent One-Step SG (OS-SG) or Two-Step SG (TS-SG) as revision surgery after AGB and primary SG (P-SG) between 2005 and 2019. Outcome criteria were perioperative complications, comorbidities, 30-day mortality, and operating time.

Results: The study analyzed data from 27,346 patients after P-SG, 320 after OS-SG, and 168 after TS-SG. Regarding the intraoperative complication, there was a significant difference in favor of P-SG and TS-SG compared to OS-SG (p < 0.001). The incidence of pulmonary complications was significantly higher in the OS-SG (p < 0.001). There was also a significant difference in occurrence of staple line stenosis in favor of TS-SG (p = 0.005) and the occurrence of sepsis (p = 0.008). The mean operating time was statistically longer in the TS-SG group than in the OS-SG group (p < 0.001). The 30-day mortality was not significantly different between the three groups (p = 0.727).

Conclusion: In general, our study shows that converting a gastric band to a SG is safe and feasible. However, lower complications were obtained with TS-SG compared to OS-SG. Despite acceptable complication and mortality rates of both procedures, we cannot recommend any surgical method as a standard procedure. Proper patient selection is crucial to avoid possible adverse effects.
A Retrospective Evaluation of Chemotherapy Overdoses in Dogs and Cats
Authors: Musser Margaret L. , Curran Kaitlin M. , Flesner Brian K. , Johannes Chad M.
Published: September 2021

Chemotherapy overdoses (ODs) are severe complications that can occur following the use of antineoplastics. However, little is known about chemotherapy ODs in veterinary medicine. The goals of this retrospective study were to report the occurrence, type, and cause of known chemotherapy ODs in companion animal medicine. The American College of Veterinary Internal Medicine oncology and internal medicine listservs were solicited for chemotherapy OD cases in dogs and cats. An OD was defined as administration of a chemotherapy dose 10% higher than intended, or at a shorter interval than planned. Twelve non-anthracycline ODs in 11 dogs, and 3 cat ODs, were collected. Overdoses in dogs included carboplatin, cyclophosphamide, L-asparaginase, lomustine, mustargen, vincristine, and vinorelbine. The cat ODs included doxorubicin and vincristine. In dogs, the median OD was 2.1x (range: 1.2–10x) the intended dose. All dogs survived the OD and developed a variety of gastrointestinal and hematologic toxicities of varying grades. Both cats with a 2.4x vincristine OD died despite supportive care. The cat who received a 2x OD of doxorubicin survived the event, experiencing Veterinary Cooperative Oncology Group–common terminology criteria for adverse events (VCOG) grade I thrombocytopenia and anemia, and VCOG grade II neutropenia. Chemotherapy ODs appear to be rare in veterinary medicine and are typically 2–3xs the intended dose. Clinical effects include VCOG grade I and II gastrointestinal distress and VCOG grade III and IV hematologic effects. With appropriate supportive care, most patients will survive the event. Life-threatening events are more common in cats following vincristine ODs.
The Impact of Entrepreneurial Education on Technology-Based Enterprises Development: The Mediating Role of Motivation
Authors: Dana Leo-Paul , Tajpour Mehdi , Salamzadeh Aidin , Hosseini Elahe , Zolfaghari Mahnaz
Published: September 2021

Technology-based enterprises play a paramount role in blooming a country economically. Nevertheless, according to a society’s capacity to launch such enterprises in various eras, their volume is less than expected in many economies. Therefore, establishing such enterprises is necessary for developing any country, although its innovation system contributes to establishing them. This paper considers the impact of entrepreneurial education on technology-based enterprise development, including motivation as a mediator variable, in Esfahan Scientific and Industrial Town. Despite much research investigating the correlation between entrepreneurial education and technology-based enterprises’ progress, it seems that no study has already considered this correlation with remarking the motivation as a mediator variable. This applied research follows a quantitative research design. The statistical population includes 500 enterprises in the Esfahan Scientific and Industrial Town, and for sampling, Cochran’s formula was applied (n = 217). Additionally, the researcher-made questionnaire and PLS3 software were used for data gathering and analysis. The results demonstrated that entrepreneurial education elements (including entrepreneurial skill, entrepreneurial learning, and entrepreneurial intention) positively affect technology-based enterprises’ development, considering motivation as a mediator variable. However, the impact of entrepreneurial intention on technology-based enterprises was not supported. It reveals that the entrepreneurial intention of motivated individuals could have a meaningful effect on the development of technology-based enterprises. Therefore, motivation is a critical issue to be considered by managers and policymakers while considering entrepreneurial education-related policies and initiatives.
Identification of the Causative Genes of Calcium Deficiency Disorders in Horticulture Crops: A Systematic Review
Authors: Kuronuma Takanori , Watanabe Hitoshi
Published: September 2021

The occurrence of calcium (Ca2+) deficiency disorders is a severe problem in the production of horticulture crops. Recently, several studies have investigated the role of gene expression in Ca2+ deficiency disorders and/or Ca2+ accumulation, providing an indication of the mechanism of Ca2+ deficiency disorders at the genetic level. To determine the relation between gene expression and the occurrence of Ca2+ deficiency disorders, we conducted a systematic review of the literature using the Preferred Reporting Items for Systematic Reviews and Meta-analyses protocol. In our initial search, we extracted studies investigating the relationships between Ca2+ deficiency disorders (tipburn and blossom-end rot) and gene expression. In our second search, we extracted studies involving functional analyses of the genes associated with Ca2+ deficiency and/or Ca2+ accumulation in plant organs. Thirty-seven articles were extracted from both searches. Studies on Ca2+ movement-related genes (Ca2+ antiporters, calreticulin, Ca2+ pumps, Ca2+ channels, and pectin methylesterases) accounted for the majority of these articles. Particularly, the effects of the expression of CAXs (Ca2+/H+ antiporters) and CRT (calreticulin) on the occurrence of Ca2+ deficiency disorders were demonstrated in studies extracted from both searches. Further research focusing on these genes may reveal the causative genes for Ca deficiency disorders in different horticulture crops. We hope that the knowledge synthesized in this systematic review will contribute to the accumulation of further knowledge and elucidation of the causes of Ca2+ deficiency disorders.