bluuebunny/crossref_metadata_2025 huggingface dataset:
The radical right in post-communist Europe. Comparative perspectives on legacies and party competition
Authors: Bustikova Lenka , Kitschelt Herbert
Published: November 2009

What role do legacies of past mobilization under late communist rule play in the success of the radical right parties in Eastern Europe? This article considers two major legacies: the legacy of national-accommodative communism and the legacy of patrimonial communism. We investigate the effect of welfare retrenchment on vote support for radical right in 2000s. Social policy reform retrenchment in universalistic welfare systems has a highly incendiary potential for political conflict and radical parties. In countries with a legacy of national accommodative communism, early differentiation of major parties on socio-cultural issues and strategies of social policy compensation kept reform losers at bay, which limited voter success of radical parties. Highly polarized patrimonial regimes, on the contrary, are the most fertile breeding ground for the radical right due to the high levels of inequality and dissatisfaction resulting from a rapid dismantling of the welfare state. The ethnic composition of countries plays an important role in the radical right mobilization as well. Radical right parties benefit from a situation in which the titular majority faces a small ethno-cultural minority.
Confessions as Proof of Innocence
Authors: Hirst Michael
Published: November 2009

In recent years, a number of criminal cases have been reported in which defendants have sought to rely upon the confessions of their co-defendants as proof of their own innocence. The co-defendant may perhaps have admitted that he alone was responsible, or that he and some third party were responsible. His confession may have been made to the police or to some other person. In one case, the father of a girl charged with robbery covertly tape-recorded admissions made to him by one of her two co-defendants. These admissions were damning to that co-defendant, but tended to exonerate the girl.
White-matter markers for psychosis in a prospective ultra-high-risk cohort
Authors: Bloemen O. J. N. , de Koning M. B. , Schmitz N. , Nieman D. H. , Becker H. E. , de Haan L. , Dingemans P. , Linszen D. H. , van Amelsvoort T. A. M. J.
Published: November 2009

Background

Subjects at ‘ultra high risk’ (UHR) for developing psychosis have differences in white matter (WM) compared with healthy controls. WM integrity has not yet been investigated in UHR subjects in relation to the development of subsequent psychosis. Hence, we investigated a prospective cohort of UHR subjects comparing whole brain fractional anisotropy (FA) of those later developing psychosis (UHR-P) to those who did not (UHR-NP).

Method

We recruited 37 subjects fulfilling UHR criteria and 10 healthy controls. Baseline 3 Tesla magnetic resonance imaging (MRI) scans and Positive and Negative Syndrome Scale (PANSS) ratings were obtained. UHR subjects were assessed at 9, 18 and 24 months for development of frank psychosis. We compared baseline FA of UHR-P to controls and UHR-NP subjects. Furthermore, we related clinical data to MRI outcome in the patient population.

Results

Of the 37 UHR subjects, 10 had transition to psychosis. UHR-P subjects showed significantly lower FA values than control subjects in medial frontal lobes bilaterally. UHR-P subjects had lower FA values than UHR-NP subjects, lateral to the right putamen and in the left superior temporal lobe. UHR-P subjects showed higher FA values, compared with UHR-NP, in the left medial temporal lobe. In UHR-P, positive PANSS negatively correlated to FA in the left middle temporal lobe. In the total UHR group positive PANSS negatively correlated to FA in the right superior temporal lobe.

Conclusions

UHR subjects who later develop psychosis have differences in WM integrity, compared with UHR subjects who do not develop psychosis and to healthy controls, in brain areas associated with schizophrenia.
Genome-wide association studies: a primer
Authors: Corvin A. , Craddock N. , Sullivan P. F.
Published: November 2009

There have been nearly 400 genome-wide association studies (GWAS) published since 2005. The GWAS approach has been exceptionally successful in identifying common genetic variants that predispose to a variety of complex human diseases and biochemical and anthropometric traits. Although this approach is relatively new, there are many excellent reviews of different aspects of the GWAS method. Here, we provide a primer, an annotated overview of the GWAS method with particular reference to psychiatric genetics. We dissect the GWAS methodology into its components and provide a brief description with citations and links to reviews that cover the topic in detail.
Continual Fascination: The Oscillating Universe in Modern Cosmology
Authors: Kragh Helge
Published: November 2009

Argument

Until the mid-nineteenth century the age-old idea of a cyclic or oscillating universe belonged to philosophical and religious discourse, with little connection to the physical and astronomical sciences. It was only with the emergence of thermodynamics, and even more so with the general theory of relativity, that it became possible to discuss the hypothesis within a scientific context. This paper follows the development of oscillating cosmological models in the twentieth century, when they were often discussed if rarely taken very seriously. How is it that this conception of the universe was seen as both fascinating and controversial? Can the persistence of such models be explained on a scientific basis alone? The idea of an oscillating universe has been in crisis several times, but recently it has experienced a spectacular revival in the form of models inspired by string theory and other theories of quantum gravity. This paper argues that the current revival of interest in cyclic models is not primarily rooted in their “aesthetic” qualities, as used to be the case. With the maturation of cosmology following the discovery of the cosmic microwave background in 1965, the hypothesis has become part of the discourse of modern theoretical cosmology, and as such it is discussed within a normal scientific context. On the other hand, extrascientific considerations continue to play a role, and they probably always will. The heritage from the past is still visible.
LIBERALISM WITHOUT HUMANISM: MICHEL FOUCAULT AND THE FREE-MARKET CREED, 1976–1979
Authors: BEHRENT MICHAEL C.
Published: November 2009

This article challenges conventional readings of Michel Foucault by examining his fascination with neoliberalism in the late 1970s. Foucault did not critique neoliberalism during this period; rather, he strategically endorsed it. The necessary cause for this approval lies in the broader rehabilitation of economic liberalism in France during the 1970s. The sufficient cause lies in Foucault's own intellectual development: drawing on his long-standing critique of the state as a model for conceptualizing power, Foucault concluded, during the 1970s, that economic liberalism, rather than “discipline,” was modernity's paradigmatic power form. Moreover, this article seeks to clarify the relationship between Foucault's philosophical antihumanism and his assessment of liberalism. Rather than arguing (as others have) that Foucault's antihumanism precluded a positive appraisal of liberalism, or that the apparent reorientation of his politics in a more liberal direction in the late 1970s entailed a partial retreat from antihumanism, this article contends that Foucault's brief, strategic, and contingent endorsement of liberalism was possible precisely because he saw no incompatibility between antihumanism and liberalism—but only liberalism of the economic variety. Economic liberalism alone, and not its political iteration, was compatible with the philosophical antihumanism that is the hallmark of Foucault's thought.
WHAT THE OCCULT REVEALS
Authors: TREITEL CORINNA
Published: November 2009

Where does occultism fit on the map of modernity? Frank Miller Turner proposed an intriguing answer in his 1974 study Between Science and Religion: The Reaction to Scientific Naturalism in Late Victorian England. The book examined the lives and struggles of six Victorian men: the philosophers Henry Sidgwick and James Ward, the scientists Alfred Russel Wallace and George John Romanes, and the writers Frederic W. H. Myers and Samuel Butler. Of the six, three cultivated a serious and sustained interest in the occult. Sidgwick and Myers engaged in psychical research, while Wallace immersed himself in phrenology and spiritualism. Raised as Christians, all of them came to find Christian belief inadequate. Yet the scientific naturalism that might have provided an alternative pole for their allegiance, that was the alternative pole of allegiance for much of their generation, failed to entice them. All had ethical qualms about its refusal to comment on God's existence or on life after death. All, too, wondered about the soul and bemoaned the reluctance of scientists to investigate the immaterial and subjective aspects of human nature. Caught between the Christianity of their upbringing and the scientific naturalism of their adulthood, Turner argued, these men “came to dwell between the science that beckoned them and the religion they had forsaken.”
MEASUREMENT AND MERITOCRACY: AN INTELLECTUAL HISTORY OF IQ
Authors: PORTER THEODORE M.
Published: November 2009

Is intelligence a fit topic for intellectual history? The creation and institutionalization of IQ (the initials have become self-sufficient, and no longer stand for “intelligence quotient”) have been a favorite topic in the history of psychology, and have even achieved some standing in social histories of class, race, and mobility, especially in the United States. The campaign to quantify intelligence tended to remove it from the domain of intellectual history, which after all has traditionally emphasized ideas and interpretations. Measurement, and not alone of the mind, was pursued as a way to rein in the intellect by making it more rigorous. What was pushed out the door, however, returned through the window in the form of debates about what intelligence means; in what sense and with what tools it can be measured; and how these measures relate to other ways of comprehending mind, thought, and reason. Quantification, a potent strategy for releasing science from the grip of history, is itself profoundly historical, as a half-century of modern scholarship has demonstrated. This historicizing of the antihistorical embodies what we may call counterreflexivity, and, as such, is partly about puncturing illusions, though it need not take a negative view of the social role of science. The perspective of history is all the more essential because the depoliticization of merit through science entails a consequential moral and political choice. Measurement, by rationalizing and stabilizing the idea of intelligence, enabled it more readily to enter everyday discourse and to be put to work in schools, businesses, and bureaucracies.
Evidence that solar wind fluctuations substantially affect the strength of dayside ionospheric convection
Authors: Kim H.‐J. , Lyons L. R. , Zou S. , Boudouridis A. , Lee D.‐Y. , Heinselman C. , McCready M.
Published: November 2009

Ionospheric convection is occasionally observed to be substantially enhanced even when the interplanetary magnetic field (IMF) is not strongly southward and the IMF By is not large. Such enhanced convection flows tend to exhibit large oscillations with ∼10–30 min periodicity. We have considered the solar wind characteristics that lead to these oscillatory convection enhancements. We have used an extensive set of Sondrestrom radar observations of ionospheric convection within the dayside polar cap. We find that IMF ULF power is closely associated with the strength of dayside convection. Convection flows during periods of large north–south IMF fluctuations are observed to be as strong as for steady and large southward IMF periods. Enhanced convection is also observed during northward IMF intervals when the interplanetary magnetic field exhibits high ULF power. We find that ULF power enhances the convection strength, independent of an observed direct effect from the solar wind speed. These observations thus suggest that IMF ULF fluctuations can significantly influence ionospheric convection. Therefore, in addition to the well‐established contributions from the direction and magnitude of the IMF and the solar wind dynamic pressure, ULF fluctuations may also be an important contributor to coupling of the solar wind to the magnetosphere‐ionosphere system. We speculate that resonance between IMF fluctuations and natural magnetospheric oscillation frequencies or magnetopause boundary oscillations might be responsible for the connection between ionospheric convection and IMF ULF power. We have also found evidence for a connection between the ULF power in the solar wind dynamic pressure and the strength of convection.
Geological Controls on Water Resource Variability in Minnesota, USA
Authors: Mooers Howard , Kanivetsky Roman , Nieber John , Peterson Heidi , Johnston Carol , Shmagin Boris
Published: November 2009

Sustainable management of water resources requires quantitative description of spatio-temporal variability, and the map is a universal medium to reflect the spatio-temporal distribution of water resources. The long history of cartography and the recent digital revolution have culminated in the Google Earth web portal with unprecedented frequency of daily use. System analysis with combination of a cyber model of landscapes, multidimensional methods of data analysis, and GIS cartography of water resources in Minnesota started in 1996 with support from faculty of Department of Geology University of Minnesota-Duluth and has continued ever since. The “Water Resource Sustainability” project, funded by the Legislative Citizens Commission on Minnesota Resources (2007-2009) was the most resent phase of the research. Research using river flow monitoring data available from USGS for Minnesota and bordering areas of North Dakota, South Dakota, Iowa and Wisconsin was completed for the territory. Analysis of landscapes properties for watersheds taken from maps - Bailey’s Ecological Provinces, Soil Taxonomy Order, topographic characteristics (average altitude, average watershed slope, total, intermittent, and perennial drainage density), thickness of quaternary sediments, and Hydrogeological Hierarchical Regionalization - revealed control of geological conditions on water resource variability. The trends of interannual patterns and seasonality of river runoff depend on bedrock type and presence or absence of thick depositions of quaternary sediments in NE and SE of research territory and also on thickness of quaternary sediments in NW. The same parts of territory have main differences in annual and February monthly yields for interval of observations 1955-1978. The numbers of river discharge yield reach difference from 5 to 20 times. The control over water resource distribution and variability belongs to geological boundaries for types of bedrocks, lithology, and thickness of quaternary sediments. Groups of watersheds recognized by mutual landscape properties (geological conditions) with statistically proven influence on hydrologic characteristics provide a basis for regionalization and creation of a water resource map. The regionalization on the water resource map opens the way to study and climate change for regional level.
Malaria Diagnosis and the Plasmodium Life Cycle: the BFO Perspective
Authors: Ceusters Werner , Smith Barry
Published: November 2009

Definitive diagnosis of malaria requires the demonstration through laboratory tests of the presence within the patient of malaria parasites or their components. Since malaria parasites can be present even in the absence of malaria, and since symptoms of malaria can be manifested even in the absence of malaria parasites, malaria diagnosis raises important issues for the adequate understanding of disease, etiology and diagnosis. One approach to the resolution of these issues adopts a realist view, according to which the needed clarifications will be derived from a careful representation of the entities on the side of the patient which form the ultimate truthmakers for clinical statements. We address a challenge to this realist approach relating to the diagnosis of malaria, and show how this challenge can be resolved by appeal to Basic Formal Ontology (BFO) and to the Ontology for General Medical Science (OGMS) constructed in its terms.
Metabolic control exerted by the 2-oxoglutarate dehydrogenase reaction: a cross-kingdom comparison of the crossroad between energy production and nitrogen assimilation
Authors: Bunik Victoria I. , Fernie Alisdair R.
Published: November 2009

Mechanism-based inhibitors and both forward and reverse genetics have proved to be essential tools in revealing roles for specific enzymatic processes in cellular function. Here, we review experimental studies aimed at assessing the impact of OG (2-oxoglutarate) oxidative decarboxylation on basic cellular activities in a number of biological systems. After summarizing the catalytic and regulatory properties of the OGDHC (OG dehydrogenase complex), we describe the evidence that has been accrued on its cellular role. We demonstrate an essential role of this enzyme in metabolic control in a wide range of organisms. Targeting this enzyme in different cells and tissues, mainly by its specific inhibitors, effects changes in a number of basic functions, such as mitochondrial potential, tissue respiration, ROS (reactive oxygen species) production, nitrogen metabolism, glutamate signalling and survival, supporting the notion that the evolutionary conserved reaction of OG degradation is required for metabolic adaptation. In particular, regulation of OGDHC under stress conditions may be essential to overcome glutamate excitotoxicity in neurons or affect the wound response in plants. Thus, apart from its role in producing energy, the flux through OGDHC significantly affects nitrogen assimilation and amino acid metabolism, whereas the side reactions of OGDHC, such as ROS production and the carboligase reaction, have biological functions in signalling and glyoxylate utilization. Our current view on the role of OGDHC reaction in various processes within complex biological systems allows us a far greater fundamental understanding of metabolic regulation and also opens up new opportunities for us to address both biotechnological and medical challenges.
Molecular biology, genetics and biochemistry of the repulsive guidance molecule family
Authors: Severyn Christopher J. , Shinde Ujwal , Rotwein Peter
Published: November 2009

RGMs (repulsive guidance molecules) comprise a recently discovered family of GPI (glycosylphosphatidylinositol)-linked cell-membrane-associated proteins found in most vertebrate species. The three proteins, RGMa, RGMb and RGMc, products of distinct single-copy genes that arose early in vertebrate evolution, are ∼40–50% identical to each other in primary amino acid sequence, and share similarities in predicted protein domains and overall structure, as inferred by ab initio molecular modelling; yet the respective proteins appear to undergo distinct biosynthetic and processing steps, whose regulation has not been characterized to date. Each RGM also displays a discrete tissue-specific pattern of gene and protein expression, and each is proposed to have unique biological functions, ranging from axonal guidance during development (RGMa) to regulation of systemic iron metabolism (RGMc). All three RGM proteins appear capable of binding selected BMPs (bone morphogenetic proteins), and interactions with BMPs mediate at least some of the biological effects of RGMc on iron metabolism, but to date no role for BMPs has been defined in the actions of RGMa or RGMb. RGMa and RGMc have been shown to bind to the transmembrane protein neogenin, which acts as a critical receptor to mediate the biological effects of RGMa on repulsive axonal guidance and on neuronal survival, but its role in the actions of RGMc remains to be elucidated. Similarly, the full spectrum of biological functions of the three RGMs has not been completely characterized yet, and will remain an active topic of ongoing investigation.
Regulation of protease‐activated receptor‐2 expression in gingival fibroblasts and Jurkat T cells by Porphyromonas gingivalis
Authors: Belibasakis Georgios N. , Bostanci Nagihan , Reddi Durga
Published: November 2009

Periodontal disease destroys the tooth‐supporting tissues as a result of chronic inflammation elicited by bacterial accumulation on tooth surfaces. Porphyromonas gingivalis is a major periodontal pathogen, with a significant capacity to perturb connective tissue homeostasis and immune responses in the periodontium, attributed to its virulence factors, including a group of secreted cysteine proteases (gingipains). PAR‐2 (protease‐activated receptor‐2) is a G‐protein‐coupled receptor activated upon proteolytic cleavage, mediating intracellular signalling events related to infection and inflammation, such as cytokine production. GF (gingival fibroblasts) and T cells have central roles in periodontal inflammation, which can potentially be mediated by PAR‐2. The aims of this study were to investigate the effects of P. gingivalis on PAR‐2 gene expression in human GF and Jurkat T cells, using quantitative real‐time PCR, and to evaluate the involvement of gingipains. After 6 h of challenge with ascending concentrations of P. gingivalis, PAR‐2 expression was up‐regulated in both cell types by approximately 5‐fold, compared with the control. The P. gingivalis concentration required for maximal PAR‐2 induction was 4‐fold greater in GF than Jurkat T cells. Heat inactivation or chemical inhibition of cysteine proteases abolished the capacity of P. gingivalis to induce PAR‐2 expression in Jurkat T cells. In conclusion, P. gingivalis can induce PAR‐2 expression in GF and Jurkat T cells, potentially attributed to its gingipains. These findings denote that P. gingivalis may perturb the host immune and inflammatory responses by enhancing PAR‐2 expression, thus contributing to the pathogenesis of periodontal disease.
Effects of auricular electrical stimulation on vagal activity in healthy men: evidence from a three-armed randomized trial
Authors: La Marca Roberto , Nedeljkovic Marko , Yuan Lizhuang , Maercker Andreas , Ehlert Ulrike
Published: November 2009

The activity of the VN (vagus nerve) is negatively associated with risk factors such as stress and smoking, morbidity and mortality. In contrast, it is also a target of therapeutic intervention. VN stimulation is used in depression and epilepsy. Because of its high invasivity and exclusive application to therapy-resistant patients, there is interest in less invasive methods affecting the VN. Several studies examining acupuncture report beneficial effects on vagal activity. However, findings are inconsistent, and applied methods are heterogeneous resulting in difficulties in interpretation. The purpose of the present study was evaluation of the effects of acupuncture on vagal activity in a three-armed randomized trial while controlling several disturbing factors. Fourteen healthy men participated in random order in four examinations: a control condition without intervention, a condition with placebo, manual acupuncture and electroacupuncture. Acupuncture was conducted on the concha of the ear, as there is neuroanatomical evidence for vagal afferents. Each examination took place once, with a week's time between examinations. RSATR (respiratory sinus arrhythmia adjusted for tidal volume) indicating vagal activity was measured continuously. The study was conducted partially blind in accordance with recommendations. After controlling for respiration, condition-specific pain sensation, individual differences in belief of acupuncture effectiveness and time effects not attributable to the interventions, electroacupuncture but not manual acupuncture was found to have a positive effect on RSATR. The results underline the potential role of auricular electrical stimulation to induce an increase in vagal activity, and it therefore might be used as preventive or adjuvant therapeutic intervention promoting health.
Phytolith analysis of Cyperaceae from the Pampean region, Argentina
Authors: Honaine Mariana Fernández , Zucol Alejandro F. , Osterrieth Margarita L.
Published: November 2009

Cyperaceae, along with Poaceae, is the main silica accumulator. Although the anatomical-taxonomic and palaeobotanical relevance of phytoliths has been well established, there are no studies that deal with the qualitative and quantitative aspects of the phytolith production or differentiate phytoliths from the different organs in Cyperaceae. Toward the construction of a detailed database of phytolith production, we describe in the present paper the phytoliths of leaves, culms and fruits of Cyperaceae from Pampean region (Argentina). Phytoliths were extracted by a calcination technique. Qualitative and quantitative characters and percentages of phytolith morphotypes were subject to principal component analysis to analyse their taxonomic relevance. For some sedges, values of phytolith content and a comparative analysis of leaves, culms and fruit phytoliths are presented here for the first time. Diverse tissues such as epidermis, xylem, parenchyma and sclerenchyma produced phytoliths. The most abundant morphotypes were conical phytoliths, which showed differences in the base shape and in their size between organs. Leaf/culm cones have a rounded, rectangular or square base; typical fruit cones have a polygonal base and they are bigger and more robust. PCA showed that quantitative and qualitative characters of cones, along with the percentages of morphotypes, allowed group distinction.
Subalpine plants show short-term positive growth responses to experimental warming and fire
Authors: Jarrad Frith C. , Wahren Carl-Henrik , Williams Richard J. , Burgman Mark A.
Published: November 2009

Climate warming has the potential to directly affect plant growth rates by accelerating plant processes, and through intermediate affects associated with increased length of the growing season and changes to soil processes. Alpine and subalpine ecosystems may be particularly vulnerable to climate warming because species are adapted to a cold environment and have limited upslope refugia in Australia. In the present study, the vegetative growth of seven subalpine open-heath species was examined in response to 3 years of warming and a wildfire. The warming experiment was established in late 2003 on the Bogong High Plains, Australia, using the protocols of the International Tundra Experiment (ITEX). During the growing seasons (snow-free periods) in 2004/2005 and 2005/2006 leaves and stems were monitored on common or widespread species from each of the major vascular plant growth forms. Plants were monitored inside and outside passively warmed open-topped chambers, at sites that were burnt in early 2003 and sites that escaped fire. In the short-term, warming had significant positive relationships with relative growth rates of three species, including Celmisia pugioniformis (forb; P = 0.09), Carex breviculmis (graminoid; P = 0.004) and Asterolasia trymalioides (shrub; P = 0.02). Burning had significant positive effects (P < 0.05) on the relative growth rates of two of these species, C. pugioniformis and C. breviculmis, as well as for Plantago euryphylla, Poa hiemata and Pimelea alpina. For P. euryphylla and P. alpina, the interaction of warming and burning showed significant relationships with relative growth rates, a negative relationship in P. euryphylla (P = 0.03) and a positive relationship in P. alpina (P = 0.07). Year and season were also found to affect the relative growth rates of most species (P < 0.05). These findings agree with previous northern hemisphere ITEX and other warming experiment results; that is, warming has a positive effect on species’ growth responses. In the present study, it is likely that continued climate warming may result in positive growth responses in other subalpine species across growth forms. Our findings emphasise the value of examining multiple species in climate-change studies.
Improvement in Lupinus luteus (Fabaceae) protoplast culture - stimulatory effect of agarose embedding and chemical nursing on protoplast divisions
Authors: Wiszniewska Alina , Pindel Anna
Published: November 2009

Comprehensive studies were undertaken to determine optimal conditions for yellow-lupin (Lupinus luteus L.) protoplast culture. The adopted isolation procedure resulted in a high yield of protoplasts obtained from hypocotyls, cotyledons and young leaves. The usefulness of liquid and agarose-solidified media was evaluated in relation to promoting survivability and morphogenetic responses of protoplasts. The influence of different plant-growth regulators and anti-oxidative agents was also examined. Cultivar ‘Parys’ was the most promising material for manipulations. The solidification of medium enhanced the development of cultures initiated from hypocotyls and cotyledons, significantly increasing the division rate. In both liquid and solid media, mitoses were arrested after the initial division and daughter cells failed to divide. An important breakthrough in the development of cultures appeared in media supplemented with 0.1% activated charcoal. Bypassing the mitosis suppression led to the formation of small aggregates from hypocotyl protoplasts. This is the first report on protocolony formation in protoplast cultures of L. luteus. Notable progress achieved here indicates that, although this species is considered as recalcitrant to in vitro manipulations, it is possible to obtain responsive cultures with higher morphogenetic potential and thus provide new material for breeding programs of lupins.
Germinability of seeds stored in capsules on plants of two myrtaceous shrubs: differences among age cohorts and between species
Authors: Kim Jae-hyeun , Walck Jeffrey L. , Hidayati Siti N. , Merritt David J. , Dixon Kingsley W.
Published: November 2009

Canopy-stored seed banks are a common trait among members of several plant families in sclerophyllous woodlands of Australia and South Africa, with their fruits usually opening in response to damage or fire. Unknown is whether the degree of dormancy and of germination differs among age cohorts in seeds stored on the mother plant. We examined the extent and speed of germination from two intensely serotinous myrtaceous species, Callistemon glaucus and Calothamnus quadrifidus, for seed held in capsules for up to 9 years. Germination of both species differed significantly among age cohorts (P < 0.0001). However, no consistent increase in germination over a range of temperatures with storage was found, suggesting that no after-ripening occurred and that seeds were non-dormant at maturity. Differences among cohorts may be due to pre-conditioning. Significant (P ≤ 0.0214) differences occurred between the small-seeded Callistemon and the large-seeded Calothamnus. Germination was (1) optimum at ≥20°C for Callistemon but at <20°C for Calothamnus, (2) 9–12 days earlier for Callistemon than for Calothamnus, and (3) higher in light than in darkness for Callistemon but equal in both light conditions for Calothamnus. While germination of the species differed in important features, we would expect synchronous germination of all age cohorts to occur following fire and the onset of regular rainfall.
Epicuticular waxes and plant primary metabolites on the surfaces of juvenile Eucalyptus globulus and E. nitens (Myrtaceae) leaves
Authors: Steinbauer Martin J. , Davies Noel W. , Gaertner Cyril , Derridj Sylvie
Published: November 2009

Our knowledge of the composition of the waxes on the surfaces of Eucalyptus leaves is growing but that of plant primary metabolites has been completely overlooked. The diffusion of primary metabolites above the cuticle exposes them to a variety of herbivorous taxa and has the potential to influence their responses to that plant. Juvenile leaves of two families of Eucalyptus globulus Labill. ssp. globulus and two families of E. nitens (Deane & Maiden) Maiden had 11 out of 16 of the epicuticular waxes that were detected in common. However, two phenylethyl esters (waxes) were only detected on leaves of one family of E. globulus and two benzyl esters (waxes) were not detected or were uncommon in samples from E. nitens. Wax compounds were generally found in samples from both leaf surfaces but a few were only detected in samples from particular sides. Species and families of eucalypt did not differ significantly in the concentrations of free sugars, polyols, malic acid or γ-aminobutyric acid (GABA) (all plant primary metabolites) collected from the surfaces of leaves. However, concentrations of all these metabolites were usually higher in collections from the upper surfaces of leaves. High wax abundance, especially on the lower surfaces of E. globulus leaves, is suspected to have hindered dissolution of all the primary metabolites quantified. Several free amino acids exhibited significant species-level differences in concentrations, namely the aromatic, amide and sulfur-containing amino acids as well as proline; family-level differences in amino acid concentrations were not significant. Australian and overseas evidence showing that differences in waxes and primary metabolites can be influential in plant susceptibility to herbivorous taxa is considered with respect to the threats posed by the autumn gum moth and Mycosphaerella leaf spot fungi.
Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry
Authors: Praça Milene Miranda , Carvalho Carlos Roberto , Novaes Carolina Ribeiro Diniz Boaventura
Published: November 2009

Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.
Number-Processing Skills in Adults with Dyslexia
Authors: Göbel Silke M. , Snowling Margaret J.
Published: November 2009

The present study investigated basic numerical skills and arithmetic in adults with developmental dyslexia. Participants performed exact and approximate calculation, basic numerical tasks (e.g., counting; symbolic number comparison; spatial–numerical association of response codes, SNARC), and visuospatial tasks (mental rotation and visual search tasks). The group with dyslexia showed a marginal impairment in counting compared to age- and IQ-matched controls, and they were impaired in exact addition, in particular with respect to speed. They were also significantly slower in multiplication. In basic number processing, however, there was no significant difference in performance between those with dyslexia and controls. Both groups performed similarly on subtraction and approximate addition tasks. These findings indicate that basic number processing in adults with dyslexia is intact. Their difficulties are restricted to the verbal code and are not associated with deficits in nonverbal magnitude representation, visual Arabic number form, or spatial cognition.
Examination of the Responses of Different Genotypes of Citrus to Huanglongbing (Citrus Greening) Under Different Conditions
Authors: Folimonova Svetlana Y. , Robertson Cecile J. , Garnsey Stephen M. , Gowda Siddarame , Dawson William O.
Published: November 2009

Citrus Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide. The causal agent of HLB in Florida is thought to be ‘Candidatus Liberibacter asiaticus’. In this work, we examined the responses of 30 different genotypes of citrus to Florida isolates of ‘Ca. L. asiaticus’ under controlled conditions in the greenhouse or growth room. Although ‘Ca. L. asiaticus’ was able to multiply in all of the plants, a wide range of responses was observed among different hosts. Based on the symptoms developed and the ability of plants to continue growth, the different genotypes were grouped into four categories: sensitive, which exhibited severe chlorosis on leaves, greatly reduced growth, and eventual death; moderately tolerant, which exhibited some scattered distinct symptoms but little or no growth reduction and no plant death; tolerant, which exhibited very minimal symptoms; and genotypes, which exhibited variable reactions. Interestingly, although ‘Ca. L. asiaticus’ was unevenly distributed within each particular plant, comparison of titers of the bacterium in different citrus genotypes revealed that most accumulated similar levels of ‘Ca. L. asiaticus’, demonstrating that there is no strict correlation between bacterial titer and severity of disease. Incubation of infected plants in the growth room with continuous light greatly affected symptoms production by reducing the time before distinctive symptoms developed and significantly increasing severity of chlorosis of leaves of all citrus genotypes. These results provide additional evidence of the correlation between disruption of phloem translocation of carbohydrates during HLB infection and the appearance of chlorotic symptoms in leaves of infected trees. We also examined interaction between ‘Ca. L. asiaticus’ and Citrus tristeza virus, which usually occurs in trees that become infected with HLB, and found no synergistic effect of the two pathogens. We trust that observations reported here will provide reagents for further examination of the ‘Ca. L. asiaticus’–citrus interaction to advance the understanding of how ‘Ca. L. asiaticus’ causes disease and to develop methods or trees to overcome the disease.
Economic Impact from Unrestricted Spread of Potato Cyst Nematodes in Australia
Authors: Hodda M. , Cook D. C.
Published: November 2009

Potato cyst nematodes (PCN) (Globodera spp.) are quarantine pests with serious potential economic consequences. Recent new detections in Australia, Canada, and the United States have focussed attention on the consequences of spread and economic justifications for alternative responses. Here, a full assessment of the economic impact of PCN spread from a small initial incursion is presented. Models linking spread, population growth, and economic impact are combined to estimate costs of spread without restriction in Australia. Because the characteristics of the Australian PCN populations are currently unknown, the known ranges of parameters were used to obtain cost scenarios, an approach which makes the model predictions applicable generally. Our analysis indicates that mean annual costs associated with spread of PCN would increase rapidly initially, associated with increased testing. Costs would then increase more slowly to peak at over AUD$20 million per year ≈10 years into the future. Afterward, this annual cost would decrease slightly due to discounting factors. Mean annual costs over 20 years were $18.7 million, with a 90% confidence interval between AUD$11.9 million and AUD$27.0 million. Thus, cumulative losses to Australian agriculture over 20 years may exceed $370 million without action to prevent spread of PCN and entry to new areas.
Mutations in a β-Tubulin Confer Resistance of Gibberella zeae to Benzimidazole Fungicides
Authors: Chen Chang-Jun , Yu Jun-Jie , Bi Chao-Wei , Zhang Yan-Nan , Xu Jian-Qiang , Wang Jian-Xin , Zhou Ming-Guo
Published: November 2009

Wheat head blight caused by Gibberella zeae (anamorph: Fusarium graminearum) is a threat to food safety in China because of mycotoxin contamination of the harvested grain, the frequent occurrence of the disease, and the failure of chemical control in some areas due to benzimidazole resistance in the pathogen population. The molecular resistance mechanism, however, of G. zeae to benzimidazole fungicides (especially carbendazim; active ingredient: methyl benzimidazol-2-yl carbamate [MBC]) is poorly understood. DNA sequences of a β-tubulin gene (β2tub) (GenBank access number FG06611.1) in G. zeae were analyzed. Mutations in β2tub in moderately resistant strains (MBCMR) included TTT (Phe)→TAT (Tyr) at codon 167 or TTC (Phe)→TAC (Tyr) at codon 200. A highly resistant strain (MBCHR) had two point mutations, one at codon 73, CAG (Gln)→CGG (Arg), and the other at codon 198, GAG (Glu)→CTG (Leu). To confirm that mutations in the β2tub confer resistance to benzimidazole fungicides, the entire β2tub locus was deleted from MBCMR and MBCHR strains of G. zeae. The resulting Δβ2tub mutants from both MBCMR and MBCHR strains grew normally on MBC-free potato dextrose agar medium and were supersensitive to MBC. Complementation of the Δβ2tub mutants by transformation with a copy of the intact β2tub locus from their parent strains exhibited less resistance than the original strains, and complementation of the Δβ2tub mutants by transformation with a copy of the intact β2tub locus from sensitive strains restored MBC sensitivity. The results indicated that the mutations in the β2tub gene conferred resistance of G. zeae to benzimidazole fungicides and this gene can be used as a genetic marker in G. zeae.
The Importance of Phenolic Metabolism to Limit the Growth of Phakopsora pachyrhizi
Authors: Lygin Anatoliy V. , Li Shuxian , Vittal Ramya , Widholm Jack M. , Hartman Glen L. , Lozovaya Vera V.
Published: November 2009

Understanding the metabolic responses of the plant to a devastating foliar disease, soybean rust, caused by Phakopsora pachyrhizi, will assist in development of cultivars resistant to soybean rust. In this study, differences in phenolic metabolism were analyzed between inoculated and noninoculated plants using two susceptible and three resistant soybean genotypes with known resistance genes. Rust infection resulted in increased accumulation of isoflavonoids and flavonoids in leaves of all soybean genotypes tested. Although the soybean phytoalexin glyceollin was not detected in leaves of uninfected plants, accumulation of this compound at marked levels occurred in rust-infected leaves, being substantially higher in genotypes with a red-brown resistant reaction. In addition, there was inhibition of P. pachyrhizi spore germination by glyceollin, formononetin, quercetin, and kaempferol. However, there was no correlation between concentrations of flavonoids quercetin and kaempferol and rust-induced isoflavonoid formononetin in soybean leaves and rust resistance. Lignin synthesis also increased in all inoculated soybean genotypes whereas there was no significant difference in all noninoculated soybean genotypes. Cell wall lignification was markedly higher in inoculated resistant lines compared with inoculated susceptible lines, indicating a possible protective role of lignin in rust infection development.
Fungus Gnat Feeding and Mechanical Wounding Inhibit Pythium aphanidermatum Infection of Geranium Seedlings
Authors: Braun S. E. , Sanderson J. P. , Nelson E. B. , Daughtrey M. L. , Wraight S. P.
Published: November 2009

A series of laboratory tests were conducted to investigate potential effects of fungus gnat (Bradysia impatiens) feeding damage on susceptibility of geranium seedlings (Pelargonium × hortorum) to infection by the root rot pathogen Pythium aphanidermatum. Effects were compared with those from similar tests in which the seedlings were mechanically wounded by severing the root tip with a scalpel. Assays of geranium seedlings in petri dishes revealed a pronounced negative fungus gnat–Pythium interaction, with exposure to fungus gnat larvae 24 h prior to inoculation with P. aphanidermatum zoospores resulting in up to 47% fewer seedling deaths than would have been expected if the two agents had acted independently. Similar results were observed when seedlings were subjected to mechanical wounding 24 h prior to zoospore inoculation. In contrast, no interaction occurred when seedlings were mechanically wounded immediately prior to inoculation. The degree of plant damage inflicted by the feeding activities of the larval fungus gnats had no significant effect on the combined damage from fungus gnats and Pythium in petri dishes. Ancillary studies showed that Pythium development on V8 agar was not inhibited by the presence of fungus gnat-associated microorganisms, nor were seedlings inoculated with these microbes less susceptible to Pythium infection. The precise mechaism or mechanisms underlying the observed interactions were not elucidated; however, the results strongly suggest that both fungus gnat feeding and mechanical wounding activated systemic defenses that made the seedlings more resistant to Pythium infection.
A randomized controlled trial of an internet‐based intervention for alcohol abusers
Authors: Cunningham John A. , Wild T. Cameron , Cordingley Joanne , Van Mierlo Trevor , Humphreys Keith
Published: November 2009

Objective  Misuse of alcohol imposes a major public health cost, yet few problem drinkers are willing to access in‐person services for alcohol abuse. The development of brief, easily accessible ways to help problem drinkers who are unwilling or unable to seek traditional treatment services could therefore have significant public health benefit. The objective of this project is to conduct a randomized controlled evaluation of the internet‐based Check Your Drinking (CYD) screener ( http://www.CheckYourDrinking.net).

Method  Participants (n = 185) recruited through a general telephone population survey were assigned randomly to receive access to the CYD, or to a no‐intervention control group.

Results  Follow‐up rates were excellent (92%). Problem drinkers provided access to the CYD displayed a six to seven drinks reduction in their weekly alcohol consumption (a 30% reduction in typical weekly drinking) at both the 3‐ and 6‐month follow‐ups compared to a one drink per week reduction among control group respondents.

Conclusions  The CYD is one of a growing number of internet‐based interventions with research evidence supporting its efficacy to reduce alcohol consumption. The internet could increase the range of help‐seeking options available because it takes treatment to the problem drinker rather than making the problem drinker come to treatment.