bluuebunny/crossref_metadata_2025 huggingface dataset:
Human Papillomavirus Infection and Vaccination: Knowledge and Attitudes among Nursing Students in Italy
Authors: Pelullo Concetta Paola , Esposito Maria Rosaria , Di Giuseppe Gabriella
Published: May 2019

This cross-sectional study assessed nursing students’ knowledge and attitudes about Human papillomavirus (HPV) infection and vaccination in Italy. The survey was conducted among a sample of 556 nursing students. Almost all reported that they had heard about HPV infection, while only 36.5% knew the risk factors of HPV infection and that this could be prevented by the HPV vaccine. Those who had heard about HPV infection during their degree program were more likely to know risk factors of HPV infection and that this could be prevented by the HPV vaccine. The majority of students (65.3%) reported that they would be willing to receive the HPV vaccine. Moreover, 91.7% of participants reported that they were willing, as future health care operators, to recommend the HPV vaccine to others. Those who knew risk factors of HPV infection and that this could be prevented by the HPV vaccine, and those who knew that cervical cancer could be prevented by the HPV vaccine expressed this positive attitude about willingness to recommend the HPV vaccine. These results highlight the need to supplement nursing students’ specific education, to improve their knowledge and awareness of HPV vaccination.
Health Status of the Elderly and Its Influence on Their Activities of Daily Living in Shangrao, Jiangxi Province
Authors: Xu Rudan , Zhou Xueqing , Cao Shiling , Huang Boshu , Wu Chiyu , Zhou Xiaojun , Lu Yuanan
Published: May 2019

To investigate the activities of daily living (ADL) and influencing factors, this survey study was conducted in Shangrao of Jiangxi. A total of 1087 elderly subjects in a long-term care (LTC) program participated in this study and their physical function, cognitive ability, self-rated health, and chronic disease were recorded during March 2017 and April 2018. The ADL scale was used to evaluate the health status of the elderly. F-test and multiple linear regression showed that the average ADL of the participants was 15.12 ± 17.59. The incidence of visual and verbal impairment was 68.6% and 14.1%, respectively. Over 74% of the elderly had severe cognitive impairment; and the prevalence of chronic disease was 84.5%. Multivariate analysis revealed that age, education, BMI (Body Mass Index), low income, verbal and cognitive ability, visual status, health self-evaluation, and some chronic diseases were related to self-care ability (p < 0.05). In summary, this study revealed that the ADL score is lower in this region and identified several influencing factors. These new findings will be useful for the local government to enhance the current LTC program for the elderly population.
Depression among Patients with HIV/AIDS: Research Development and Effective Interventions (GAPRESEARCH)
Authors: Tran Bach Xuan , Ho Roger C. M. , Ho Cyrus S. H. , Latkin Carl A. , Phan Hai Thanh , Ha Giang Hai , Vu Giang Thu , Ying Jiangbo , Zhang Melvyn W. B.
Published: May 2019

Depression in people living with HIV (PLWH) has become an urgent issue and has attracted the attention of both physicians and epidemiologists. Currently, 39% of HIV patients are reported to suffer from depression. This population is more likely to experience worsening disease states and, thus, poorer health outcomes. In this study, we analyzed research growth and current understandings of depression among HIV-infected individuals. The number of papers and their impacts have been considerably grown in recent years, and a total of 4872 publications published from 1990–2017 were retrieved from the Web of Science database. Research landscapes related to this research field include risk behaviors and attributable causes of depression in HIV population, effects of depression on health outcomes of PLWH, and interventions and health services for these particular subjects. We identified a lack of empirical studies in countries where PLWH face a high risk of depression, and a modest level of interest in biomedical research. By demonstrating these research patterns, highlighting the research gaps and putting forward implications, this study provides a basis for future studies and interventions in addressing the critical issue of HIV epidemics.
Associations between Meteorological Factors and Visceral Leishmaniasis Outbreaks in Jiashi County, Xinjiang Uygur Autonomous Region, China, 2005–2015
Authors: Li Yi , Zheng Canjun
Published: May 2019

Although visceral leishmaniasis disease is controlled overall in China, it remains a serious public health problem and remains fundamentally uncontrolled in Jiashi County, Xinjiang Uygur Autonomous Region. During 2005–2015, there were two outbreaks in Jiashi County. Assessing the influence of meteorological factors on visceral leishmaniasis incidence is essential for its monitoring and control. In this study, we applied generalized estimating equations to assess the impact of meteorological factors on visceral leishmaniasis risk from 2005 to 2015. We also compared meteorological factors among years with Kruskal–Wallis test to explore possible reasons behind the two outbreaks that occurred during our study period. We found that temperature and relative humidity had very significant associations with visceral leishmaniasis risk and there were interactions between these factors. Increasing temperature or decreasing relative humidity could increase the risk of visceral leishmaniasis events. The outbreaks investigated might have been related to low relative humidity and high temperatures. Our findings will support the rationale for visceral leishmaniasis control in China.
Performance and Pacing of Age Groups in Half-Marathon and Marathon
Authors: Nikolaidis Pantelis Theodoros , Cuk Ivan , Rosemann Thomas , Knechtle Beat
Published: May 2019

The aim of the present study was to examine the age-related differences in performance and pacing in a half-marathon compared to a marathon. All finishers (n = 9137) in the Ljubljana 2017 half-marathon (n = 7258) and marathon (n = 1853) with available data on split times during the races, were analysed for pacing. Half-marathoners were slower than marathoners among women, (2.77 ± 0.35 versus 2.86 ± 0.39 m·s−1 respectively, p < 0.001), but faster among men (3.14 ± 0.45 versus 3.08 ± 0.46 m·s−1 respectively, p < 0.001). In both race distances, the <25 age group was the fastest and the >54 age group the slowest (p < 0.001). All age groups presented a positive pacing in both race distances and genders, with each segment being slower than the previous one. However, an end spurt was observed in the marathon, but not in the half-marathon. A more even pace in the half-marathon than in the marathon was shown for most age groups. In summary, age-group finishers in the half-marathon decreased running speed across the race, presented a more even pacing than marathoners, and did not show an end spurt.
Dietary and Activity Factors Influence Poor Sleep and the Sleep-Obesity Nexus among Children
Authors: Morrissey Bridget , Allender Steven , Strugnell Claudia
Published: May 2019

Background: Behavioral factors such as physical activity, sedentary behavior and diet have previously been found to be key modifiable determinants of childhood overweight and obesity, yet require further investigation to provide an understanding of their potential influence on sleep outcomes along with the sleep-obesity nexus. Methods: The study included 2253 students (ages 8.8–13.5) from two monitoring studies across regional Victoria. Students completed a self-report electronic questionnaire on demographic characteristics, health behaviors (including sleep, physical activity, screen time and diet) and well-being, and were invited to have anthropometric measurements (height and weight) taken. Regression models were used to assess the associations between sleep, behavioral factors and BMI z-scores. Results: Screen time (particularly in bed) and sugar-sweetened beverage (SSB) consumption were shown to increase the likelihood of having more than three sleep problems, while physical activity and other dietary factors were not. After controlling for these behaviors, significance remained for having two or more than three sleep problems and an increased odds of overweight/obesity. Conclusions: This study highlights how the usage of screen devices and SSB consumption behaviors might influence children’s weight status via the sleep-obesity nexus.
Combining Water Fraction and DEM-Based Methods to Create a Coastal Flood Map: A Case Study of Hurricane Harvey
Authors: Li Xiaoxuan , Cummings Anthony , Alruzuq Ali , Matyas Corene , Amanambu Amobichukwu
Published: May 2019

Tropical cyclones are incredibly destructive and deadly, inflicting immense losses to coastal properties and infrastructure. Hurricane-induced coastal floods are often the biggest threat to life and the coastal environment. A quick and accurate estimation of coastal flood extent is urgently required for disaster rescue and emergency response. In this study, a combined Digital Elevation Model (DEM) based water fraction (DWF) method was implemented to simulate coastal floods during Hurricane Harvey on the South Texas coast. Water fraction values were calculated to create a 15 km flood map from multiple channels of the Advanced Technology Microwave Sound dataset. Based on hydrological inundation mechanism and topographic information, the coarse-resolution flood map derived from water fraction values was then downscaled to a high spatial resolution of 10 m. To evaluate the DWF result, Storm Surge Hindcast product and flood-reported high-water-mark observations were used. The results indicated a high overlapping area between the DWF map and buffered flood-reported high-water-marks (HWMs), with a percentage of more than 85%. Furthermore, the correlation coefficient between the DWF map and CERA SSH product was 0.91, which demonstrates a strong linear relationship between these two maps. The DWF model has a promising capacity to create high-resolution flood maps over large areas that can aid in emergency response. The result generated here can also be useful for flood risk management, especially through risk communication.
Obstacle-Aware Indoor Pathfinding Using Point Clouds
Authors: Díaz-Vilariño Lucía , Boguslawski Pawel , Khoshelham Kourosh , Lorenzo Henrique
Published: May 2019

With the rise of urban population, updated spatial information of indoor environments is needed in a growing number of applications. Navigational assistance for disabled or aged people, guidance for robots, augmented reality for gaming, and tourism or training emergency assistance units are just a few examples of the emerging applications requiring real three-dimensional (3D) spatial data of indoor scenes. This work proposes the use of point clouds for obstacle-aware indoor pathfinding. Point clouds are firstly used for reconstructing semantically rich 3D models of building structural elements in order to extract initial navigational information. Potential obstacles to navigation are classified in the point cloud and directly used to correct the path according to the mobility skills of different users. The methodology is tested in several real case studies for wheelchair and ordinary users. Experiments show that, after several iterations, paths are readapted to avoid obstacles.
Anisotropic Diffusion for Improved Crime Prediction in Urban China
Authors: Tang Yicheng , Zhu Xinyan , Guo Wei , Wu Ling , Fan Yaxin
Published: May 2019

As a major social issue during urban development, crime is closely related to socioeconomic, geographic, and environmental factors. Traditional crime prediction models reveal the spatiotemporal dynamics of crime risks, but usually ignore the environmental context of the geographic areas where crimes occur. Therefore, it is difficult to enhance the spatial accuracy of crime prediction. We propose the use of anisotropic diffusion to include environmental factors of the evaluated geographic area in the traditional crime prediction model, thereby aiming to predict crime occurrence at a finer scale regarding spatiotemporal aspects and environmental similarity. Under different evaluation criteria, the average prediction accuracy of the proposed method is 28.8%, improving prediction accuracy by 77.5%, as compared to the traditional methods. The proposed method can provide strong policing support in terms of conducting targeted hotspot policing and fostering sustainable community development.
Molecular Advances on Phytases in Barley and Wheat
Authors: Madsen Claus Krogh , Brinch-Pedersen Henrik
Published: May 2019

Phytases are pro-nutritional enzymes that hydrolyze phytate and make associated nutrients, such as phosphorous, iron, and zinc, bioavailable. Single-stomached animals and humans depend on phytase supplied through the diet or the action of phytase on the food before ingestion. As a result, phytases—or lack thereof—have a profound impact on agricultural ecosystems, resource management, animal health, and public health. Wheat, barley and their Triticeae relatives make exceptionally good natural sources of phytase. This review highlights advances in the understanding of the molecular basis of the phytase activity in wheat and barley, which has taken place over the past decade. It is shown how the phytase activity in the mature grains of wheat and barley can be ascribed to the PAPhy_a gene, which exists as a single gene in barley and in two or three homeologous copies in tetra- and hexaploid wheat, respectively. It is discussed how understanding the function and regulation of PAPhy_a may support the development of improved wheat and barley with even higher phytase activity.
Structural Insights into the Molecular Evolution of the Archaeal Exo-β-d-Glucosaminidase
Authors: Mine Shouhei , Watanabe Masahiro
Published: May 2019

The archaeal exo-β-d-glucosaminidase (GlmA), a thermostable enzyme belonging to the glycosidase hydrolase (GH) 35 family, hydrolyzes chitosan oligosaccharides into monomer glucosamines. GlmA is a novel enzyme in terms of its primary structure, as it is homologous to both GH35 and GH42 β-galactosidases. The catalytic mechanism of GlmA is not known. Here, we summarize the recent reports on the crystallographic analysis of GlmA. GlmA is a homodimer, with each subunit comprising three distinct domains: a catalytic TIM-barrel domain, an α/β domain, and a β1 domain. Surprisingly, the structure of GlmA presents features common to GH35 and GH42 β-galactosidases, with the domain organization resembling that of GH42 β-galactosidases and the active-site architecture resembling that of GH35 β-galactosidases. Additionally, the GlmA structure also provides critical information about its catalytic mechanism, in particular, on how the enzyme can recognize glucosamine. Finally, we postulate an evolutionary pathway based on the structure of an ancestor GlmA to extant GH35 and GH42 β-galactosidases.
Systemic Inflammation in Metabolic Syndrome: Increased Platelet and Leukocyte Activation, and Key Role of CX3CL1/CX3CR1 and CCL2/CCR2 Axes in Arterial Platelet-Proinflammatory Monocyte Adhesion
Authors: Marques Patrice , Collado Aida , Martinez-Hervás Sergio , Domingo Elena , Benito Esther , Piqueras Laura , Real José T. , Ascaso Juan F. , Sanz Maria-Jesus
Published: May 2019

Background: Metabolic syndrome is associated with low-grade systemic inflammation, which is a key driver of premature atherosclerosis. We characterized immune cell behavior in metabolic syndrome, its consequences, and the potential involvement of the CX3CL1/CX3CR1 and CCL2/CCR2 chemokine axes. Methods: Whole blood from 18 patients with metabolic syndrome and 21 age-matched controls was analyzed by flow cytometry to determine the leukocyte immunophenotypes, activation, platelet-leukocyte aggregates, and CX3CR1 expression. ELISA determined the plasma marker levels. Platelet-leukocyte aggregates adhesion to tumor necrosis factor-α (TNFα)-stimulated arterial endothelium and the role of CX3CL1/CX3CR1 and CCL2/CCR2 axes was investigated with the parallel-plate flow chamber. Results: When compared with the controls, the metabolic syndrome patients presented greater percentages of eosinophils, CD3+ T lymphocytes, Mon2/Mon3 monocytes, platelet-eosinophil and -lymphocyte aggregates, activated platelets, neutrophils, eosinophils, monocytes, and CD8+ T cells, but lower percentages of Mon1 monocytes. Patients had increased circulating interleukin-8 (IL-8) and TNFα levels and decreased IL-4. CX3CR1 up-regulation in platelet-Mon1 monocyte aggregates in metabolic syndrome patients led to increased CX3CR1/CCR2-dependent platelet-Mon1 monocyte adhesion to dysfunctional arterial endothelium. Conclusion: We provide evidence of generalized immune activation in metabolic syndrome. Additionally, CX3CL1/CX3CR1 or CCL2/CCR2 axes are potential candidates for therapeutic intervention in cardiovascular disorders in metabolic syndrome patients, as their blockade impairs the augmented arterial platelet-Mon1 monocyte aggregate adhesiveness, which is a key event in atherogenesis.
Circulating Soluble CD36 is Similar in Type 1 and Type 2 Diabetes Mellitus versus Non-Diabetic Subjects
Authors: Castelblanco Esmeralda , Sanjurjo Lucía , Falguera Mireia , Hernández Marta , Fernandez-Real José-Manuel , Sarrias Maria-Rosa , Alonso Nuria , Mauricio Didac
Published: May 2019

The aim of this study was to determine whether plasma concentrations of sCD36 (soluble CD36) are associated with the presence of type 1 or type 2 diabetes. Plasma levels of sCD36 were analysed in 1023 subjects (225 type 1 diabetes (T1D) patients, 276 type 2 diabetes (T2D) patients, and 522 non-diabetic control subjects) using an enzyme-linked immunosorbent assay (ELISA). Multinomial and logistic regression models were performed to evaluate associations with sCD36 and its association with diabetes types. There were no significant differences in sCD36 (p = 0.144) among study groups, neither in head-to-head comparisons: non-diabetic versus T1D subjects (p = 0.180), non-diabetic versus T2D subjects (p = 0.583), and T1D versus T2D patients (p = 0.151). In the multinomial model, lower sCD36 concentrations were associated with older age (p < 0.001), tobacco exposure (p = 0.006), T2D (p = 0.020), and a higher-platelets count (p = 0.004). However, in logistic regression models of diabetes, sCD36 showed only a weak association with T2D. The current findings show a weak association of circulating sCD36 with type 2 diabetes and no association with T1D.
Decreasing Trends of Secondary Primary Colorectal Cancer among Women with Uterine Cancer: A Population-Based Analysis
Authors: Matsuo Koji , Mandelbaum Rachel S. , Machida Hiroko , Yoshihara Kosuke , Muggia Franco M. , Roman Lynda D. , Wright Jason D.
Published: May 2019

The current study examined trends, characteristics, and outcomes of women with uterine cancer who had secondary colorectal cancer. This is a retrospective study utilizing the Surveillance, Epidemiology, and End Results Program between 1973–2013. Among uterine cancer (n = 246,272) and colorectal cancer (n = 421,312) cohorts, women with both diagnoses were identified, and clinico-pathological factors and survival were extracted and analyzed. There were 6862 women with both cancer diagnoses, representing 2.8% of the uterine cancer cohort and 1.6% of the colorectal cancer cohort. Among 123,940 women with uterine cancer survivors, the number with postcedent colorectal cancer decreased from 5.3% to 0.7% between 1981–2008 (relative risk reduction 87.0% p < 0.001). Similarly, of 141,801 women with colorectal cancer survivors, the number with postcedent uterine cancer decreased from 1.7% to 0.5% between 1973–2008 (relative risk reduction 71.6%, p < 0.001). In the uterine cancer cohort, women with antecedent/synchronous colorectal cancer had more high-grade tumors and advanced-stage disease resulting in poorer survival, whereas those who had postcedent colorectal cancer had more low-grade tumors and early-stage disease resulting in superior survival compared to those without secondary colorectal cancer (all, p < 0.05). In conclusion, the development of postcedent colorectal cancer following uterine cancer has decreased in recent years in the United States.
Very Low Protein Diet for Patients with Chronic Kidney Disease: Recent Insights
Authors: Di Micco Lucia , Di Lullo Luca , Bellasi Antonio , Di Iorio Biagio R.
Published: May 2019

Use of nutritional therapy (NT) in chronic kidney disease (CKD) patients is still debated among nephrologists, but it represents a fundamental point in the conservative treatment of CKD. It has been used for years and it has new goals today, such as (1) the reduction of edema, diuretics, and blood pressure values with a low sodium-content diet; (2) the dose reduction of phosphate levels and phosphate binders; (3) the administration of bicarbonate with vegetables in order to correct metabolic acidosis and delay CKD progression; (4) the reduction of the number and the doses of drugs and chemical substances; and (5) the lowering of urea levels, the cure of intestinal microbioma, and the reduction of cyanates levels (such as indoxyl-sulphate and p-cresol sulphate), which are the most recent known advantages achievable with NT. In conclusion, NT and especially very low protein diet (VLPD) have several beneficial effects in CKD patients and slows the progression of CKD.
Methods for Assessment and Monitoring of Light Pollution around Ecologically Sensitive Sites
Authors: Barentine John C.
Published: May 2019

Since the introduction of electric lighting over a century ago, and particularly in the decades following the Second World War, indications of artificial light on the nighttime Earth as seen from Earth orbit have increased at a rate exceeding that of world population growth during the same period. Modification of the natural photic environment at night is a clear and imminent consequence of the proliferation of anthropogenic light at night into outdoor spaces, and with this unprecedented change comes a host of known and suspected ecological consequences. In the past two decades, the conservation community has gradually come to view light pollution as a threat requiring the development of best management practices. Establishing those practices demands a means of quantifying the problem, identifying polluting sources, and monitoring the evolution of their impacts through time. The proliferation of solid-state lighting and the changes to source spectral power distribution it has brought relative to legacy lighting technologies add the complication of color to the overall situation. In this paper, I describe the challenge of quantifying light pollution threats to ecologically-sensitive sites in the context of efforts to conserve natural nighttime darkness, assess the current state of the art in detection and imaging technology as applied to this realm, review some recent innovations, and consider future prospects for imaging approaches to provide substantial support for darkness conservation initiatives around the world.
Improved Double-Layer Soil Consolidation Theory and Its Application in Marine Soft Soil Engineering
Authors: Chen Deqiang , Luo Junhui , Liu Xianlin , Mi Decai , Xu Longwang
Published: May 2019

Marine soft soil foundation is a double-layer foundation structure with a crust layer and soft substratum. Moreover, it is common that there are various forms of drainage. Accordingly, based on Terzaghi’s consolidation theory and the continuous drainage boundary conditions theory of controllable drainage conditions, an improved double-layer soil consolidation theory considering continuous drainage boundary conditions was proposed. To improve the computational efficiency and accuracy, the Laplace transform and the Stehfest algorithm was used to deduce the numerical solution of the improved double-layer soil consolidation theory considering continuous drainage boundary conditions and to compile a computer program. Subsequently, the theory was validated and analyzed by the degenerated model of the perfectly permeable boundary conditions and the semi-permeable boundary conditions, respectively, which showed that this theory has higher accuracy. Simultaneously, the analysis of double-layer consolidation settlement under continuous drainage boundary conditions for marine soft soil foundation of Guangxi Binhai Highway was carried on. The result showed that the consolidation settlement calculated by the improved double-layer consolidation theory presented is basically consistent with the field measurement results, and that the correlation coefficient between them is higher. Accordingly, the research results can provide useful basic information for marine soft foundation engineering.
A Theoretical Framework and Conceptual Design for Engaging Children in Therapy at Home—The Design of a Wearable Breathing Trainer
Authors: Siering Lara , Ludden Geke D.S. , Mader Angelika , van Rees Hellen
Published: May 2019

Wearable technologies are being implemented in the health and medical context with increasing frequency. Such technologies offer valuable opportunities to stimulate self-management in these domains. In this context, engagement plays a crucial role. An engaged patient is a patient who is emotionally involved and committed to the therapy or care process. Particularly for children who have to follow some sort of therapy, engagement is important to ensure a successful outcome of the therapy. To design for engagement, a framework based on theories of motivation in child therapy was developed. This framework was applied to the design of a wearable breathing trainer for children with asthma and dysfunctional breathing. As such, the present paper provides knowledge about the implementation of theory on engagement and motivation in design. Expert and first user evaluations found that the resulting prototype is appealing, perceived as useful, and may engage children in breathing training and stimulate self-management.
Landscape Notions among Greek Engineering Students: Exploring Landscape Perceptions, Knowledge and Participation
Authors: Terkenli Theano S. , Daras Tryfon , Maria Efpraxia-Aithra
Published: May 2019

The objective of this paper is to explore and critically analyze the basic notions of landscape and their change through time, among Greek engineering students, from all academically formative years of their undergraduate studies, at the Technical University of Crete. Specifically, it probes into their perspectives vis-à-vis the landscape at large and their everyday-life landscapes in particular, regarding their landscape perceptions, behavior, and education. This study takes place in two stages (2012 and 2017) and is placed in the context of continued scientific investigation into the interrelationships of various “publics” with various types of landscapes and landscape development ideas, perceptions, and preferences—and specifically those professionals-in-the-making who are bound to become key future agents in Greek landscape stewardship. Our aims serve the European Landscape Convention’s purposes of landscape research, education, and awareness-raising; they also cater to the need for geographically targeted place-specific application of the European Landscape Convention (ELC). Our findings reaffirm widely and long-held landscape notions, emphasizing the natural, the visual, and the aesthetic in landscape perception and conceptualization, but also point to landscape education deficiencies in the Greek educational system. These constitute significant findings in the context of the country’s efforts to lay out the blueprints for its future landscapes, by contributing to Greek lay landscape awareness and conscience building, but especially by informing future landscape-related professionals.
Large Contractions on Quasi-Metric Spaces with an Application to Nonlinear Fractional Differential Equations
Authors: Karapınar Erdal , Fulga Andreea , Rashid Maliha , Shahid Lariab , Aydi Hassen
Published: May 2019

In this manuscript, we introduce a new notion: a Berinde type ( α , ψ ) -contraction mapping. Thereafter, we investigate not only the existence, but also the uniqueness of a fixed point of such mappings in the setting of right-complete quasi-metric spaces. The result, presented here, not only generalizes a number of existing results, but also unifies several ones on the topic in the literature. An application of nonlinear fractional differential equations is given.
Common Fixed Point under Nonlinear Contractions on Quasi Metric Spaces
Authors: Shatanawi Wasfi , Abodayeh Kamaleldin
Published: May 2019

We introduce in this article the notion of ( ψ , ϕ ) - quasi contraction for a pair of functions on a quasi-metric space. We also investigate the existence and uniqueness of the fixed point for a couple functions under that contraction.
Profiling of Small Molecular Metabolites in Nostoc flagelliforme during Periodic Desiccation
Authors: Gao Xiang , Liu Bin , Ji Boyang
Published: May 2019

The mass spectrometry-based metabolomics approach has become a powerful tool for the quantitative analysis of small-molecule metabolites in biological samples. Nostoc flagelliforme, an edible cyanobacterium with herbal value, serves as an unexploited bioresource for small molecules. In natural environments, N. flagelliforme undergoes repeated cycles of rehydration and dehydration, which are interrupted by either long- or short-term dormancy. In this study, we performed an untargeted metabolite profiling of N. flagelliforme samples at three physiological states: Dormant (S1), physiologically fully recovered after rehydration (S2), and physiologically partially inhibited following dehydration (S3). Significant metabolome differences were identified based on the OPLS-DA (orthogonal projections to latent structures discriminant analysis) model. In total, 183 differential metabolites (95 up-regulated; 88 down-regulated) were found during the rehydration process (S2 vs. S1), and 130 (seven up-regulated; 123 down-regulated) during the dehydration process (S3 vs. S2). Thus, it seemed that the metabolites’ biosynthesis mainly took place in the rehydration process while the degradation or possible conversion occurred in the dehydration process. In addition, lipid profile differences were particularly prominent, implying profound membrane phase changes during the rehydration–dehydration cycle. In general, this study expands our understanding of the metabolite dynamics in N. flagelliforme and provides biotechnological clues for achieving the efficient production of those metabolites with medical potential.
Could Hyperbaric Oxygen Be a Solution in the Treatment of Spinal Infections?
Authors: Körpınar Şefika
Published: May 2019

Background and Objective: Pyogenic spinal infections are rare and potentially devastating, requiring prompt recognition and management. Parallel to the ever-increasing number of invasive spinal procedures, its incidence is on a steady rise, particularly in an expanding elderly population. The aim of this study was to evaluate the efficacy of hyperbaric oxygen (HBO2) therapy in the treatment of this heterogeneous group of disorders. Materials and Methods: Nineteen patients who were referred to our center for HBO2 with a clinical diagnosis of spinal infections (vertebral osteomyelitis, pyogenic spondylitis, spondylodiscitis, surgical site infection following spine surgery, epidural abscess) were retrospectively reviewed. Results: Infection resolution was adequately achieved in 12 of 13 patients (92.3%) on magnetic resonance imaging at the end of HBO2 treatment or during the first month of follow-up. The mean follow-up period was 11 months (range 1 month to 3 years). Conclusions: This study suggests that HBO2 therapy is efficacious in patients with pyogenic spinal infections complicated by primary therapy failure or by medical comorbidities that may impede the eradication of microbial infection and delay wound healing. HBO2 therapy may be useful for reducing long hospital stays, repeated surgeries, and morbidities.
Dietary and Pharmacological Treatment of Nonalcoholic Fatty Liver Disease
Authors: Jeznach-Steinhagen Anna , Ostrowska Joanna , Czerwonogrodzka-Senczyna Aneta , Boniecka Iwona , Shahnazaryan Urszula , Kuryłowicz Alina
Published: May 2019

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the developed world. Simple hepatic steatosis is mild, but the coexistence of steatohepatitis (NASH) and fibrosis increases the risk of hepatocellular carcinoma. Proper dietary and pharmacological treatment is essential for preventing NAFLD progression. The first-line treatment should include dietary intervention and increased physical activity. The diet should be based on the food pyramid, with a choice of products with low glycemic index, complex carbohydrates in the form of low-processed cereal products, vegetables, and protein-rich products. Usage of insulin-sensitizing substances, pro- and prebiotics, and vitamins should also be considered. Such a therapeutic process is intended to support both liver disease and obesity-related pathologies, including insulin resistance, diabetes, dyslipidemia, and blood hypertension. In the pharmacological treatment of NAFLD, apart from pioglitazone, there are new classes of antidiabetic drugs that are of value, such as glucagon-like peptide 1 analogs and sodium/glucose cotransporter 2 antagonists, while several other compounds that target different pathogenic pathways are currently being tested in clinical trials. Liver biopsies should only be considered when there is a lack of decline in liver enzymes after 6 months of the abovementioned treatment. Dietary intervention is recommended in all patients with NAFLD, while pharmacological treatment is recommended especially for those with NASH and showing significant fibrosis in a biopsy.
In Vitro Effect of the Traditional Medicine Hainosan (Painongsan) on Porphyromonas gingivalis
Authors: Minami Masaaki , Takase Hiroshi , Taira Masayo , Makino Toshiaki
Published: May 2019

Background: Hainosan (Painongsan) is a traditional Japanese and Chinese medicine that is used to treat several purulent diseases, including gingivitis and periodontitis. This formulation contains three crude drug components: The dried immature fruit of Citrus aurantium (Aurantii Fructus Immaturus), the dried root of Paeonia lactiflora (Paeoniae Radix), and the dried root of Platycodon grandiflorum (Platycodi Radix). Here we evaluated the in vitro antibacterial effects of hainosan extract (HNS) and extracts of its three components against Porphyromonas gingivalis, one of the pathogenic bacteria that causes periodontitis. Methods: The antibacterial activities of HNS and its components were examined by counting the number of colony-forming units (CFUs) and through transmission electron microscopy. Results: We found that HNS had direct antibacterial activity against three P. gingivalis isolates (JCM12257, JCM8525, and JCM19600), with HNS-treated cells being significantly smaller than those of untreated bacteria. Extracts of Platycodi Radix and Paeoniae Radix significantly suppressed the growth of P. gingivalis in a dose-dependent manner, with Platycodi Radix extract having the greatest antibacterial effect. In addition, P. gingivalis that were treated with Platycodi Radix extract were significantly larger than those treated with Aurantii Fructus Immaturus or Paeoniae Radix extracts. Further analysis showed that platycodin D, which is one of the ingredients of Platycodi Radix, reduced bacterial growth. Conclusions: Platycodi Radix is the active component in Hainosan and may represent a useful agent for the treatment of P. gingivalis-induced gingivitis and periodontitis.
A New Time–Frequency Feature Extraction Method for Action Detection on Artificial Knee by Fractional Fourier Transform
Authors: Wang Tianrun , Liu Ning , Su Zhong , Li Chao
Published: May 2019

With the aim of designing an action detection method on artificial knee, a new time–frequency feature extraction method was proposed. The inertial data were extracted periodically using the microelectromechanical systems (MEMS) inertial measurement unit (IMU) on the prosthesis, and the features were extracted from the inertial data after fractional Fourier transform (FRFT). Then, a feature vector composed of eight features was constructed. The transformation results of these features after FRFT with different orders were analyzed, and the dimensions of the feature vector were reduced. The classification effects of different features and different orders are analyzed, according to which order and feature of each sub-classifier were designed. Finally, according to the experiment with the prototype, the method proposed above can reduce the requirements of hardware calculation and has a better classification effect. The accuracies of each sub-classifier are 95.05%, 95.38%, 91.43%, and 89.39%, respectively; the precisions are 78.43%, 98.36%, 98.36%, and 93.41%, respectively; and the recalls are 100%, 93.26%, 86.96%, and 86.68%, respectively.
The Role of Organic Matter on Uranium Precipitation in Zoovch Ovoo, Mongolia
Authors: Rallakis Dimitrios , Michels Raymond , Brouand Marc , Parize Olivier , Cathelineau Michel
Published: May 2019

The Zoovch Ovoo uranium deposit is located in East Gobi Basin in Mongolia. It is hosted in the Sainshand Formation, a Late Cretaceous siliciclastic reservoir, in the lower part of the post-rift infilling of the Mesozoic East Gobi Basin. The Sainshand Formation corresponds to poorly consolidated medium-grained sandy intervals and clay layers deposited in fluvial-lacustrine settings. The uranium deposit is confined within a 60- to 80-m-thick siliciclastic reservoir inside aquifer driven systems, assimilated to roll-fronts. As assessed by vitrinite reflectance (%Rr < 0.4) and molecular geochemistry, the formation has never experienced significant thermal maturation. Detrital organic matter (type III and IV kerogens) is abundant in the Zoovch Ovoo depocenter. In this framework, uranium occurs as: (i) U-rich macerals without any distinguishable U-phase under SEM observation, containing up to 40 wt % U; (ii) U expressed as UO2 at the rims of large (several millimeters) macerals and (iii) U oxides partially to entirely replacing macerals, while preserving the inherited plant texture. Thus, uranium is accumulated gradually in the macerals through an organic carbon–uranium epigenization process, in respect to the maceral’s chemistry and permeability. Most macerals are rich in S and, to a lesser extent, in Fe. Frequently, Fe and S contents do not fit the stoichiometry of pyrite, although pyrite also occurs as small inclusions within the macerals. The organic matter appears thus as a major redox trap for uranium in this kind of geological setting.
Aza-Amino Acids Disrupt β-Sheet Secondary Structures
Authors: McMechen Michael A. , Willis Evan L. , Gourville Preston C. , Proulx Caroline
Published: May 2019

Cα to N substitution in aza-amino acids imposes local conformational constraints, changes in hydrogen bonding properties, and leads to adaptive chirality at the nitrogen atom. These properties can be exploited in mimicry and stabilization of peptide secondary structures and self-assembly. Here, the effect of a single aza-amino acid incorporation located in the upper β-strand at a hydrogen-bonded (HB) site of a β-hairpin model peptide (H-Arg-Tyr-Val-Glu-Val-d-Pro-Gly-Orn-Lys-Ile-Leu-Gln-NH2) is reported. Specifically, analogs in which valine3 was substituted for aza-valine3 or aza-glycine3 were synthesized, and their β-hairpin stabilities were examined using Nuclear Magnetic Resonance (NMR) spectroscopy. The azapeptide analogs were found to destabilize β-hairpin formation compared to the parent peptide. The aza-valine3 residue was more disruptive of β-hairpin geometry than its aza-glycine3 counterpart.
Optimization of Cation Exchange for the Separation of Actinium-225 from Radioactive Thorium, Radium-223 and Other Metals
Authors: Fitzsimmons Jonathan , Foley Bryan , Torre Bryna , Wilken Megan , Cutler Cathy S. , Mausner Leonard , Medvedev Dmitri
Published: May 2019

Actinium-225 (225Ac) can be produced with a linear accelerator by proton irradiation of a thorium (Th) target, but the Th also underdoes fission and produces 400 other radioisotopes. No research exists on optimization of the cation step for the purification. The research herein examines the optimization of the cation exchange step for the purification of 225Ac. The following variables were tested: pH of load solution (1.5–4.6); rinse steps with various concentrations of HCl, HNO3, H2SO4, and combinations of HCl and HNO3; various thorium chelators to block retention; MP50 and AG50 resins; and retention of 20–45 elements with different rinse sequences. The research indicated that HCl removes more isotopes earlier than HNO3, but that some elements, such as barium and radium, could be eluted with ≥2.5 M HNO3. The optimal pH of the load solution was 1.5–2.0, and the optimized rinse sequence was five bed volumes (BV) of 1 M citric acid pH 2.0, 3 BV of water, 3 BV of 2 M HNO3, 6 BV of 2.5 M HNO3 and 20 BV of 6 M HNO3. The sequence recovered >90% of 225Ac with minimal 223Ra and thorium present.
Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
Authors: Nagai Noriaki , Iwai Yoshie , Deguchi Saori , Otake Hiroko , Kanai Kazutaka , Okamoto Norio , Shimomura Yoshikazu
Published: May 2019

We previously found the instillation of sericin to be useful as therapy for keratopathy with or without diabetes mellitus. In this study, we investigated whether a combination of solid magnesium hydroxide nanoparticles (MHN) enhances epithelial corneal wound healing by sericin using rabbits, normal rats and type 2 diabetes mellitus rats with debrided corneal epithelium (ex vivo and in vivo studies). Ophthalmic formulations containing sericin and MHN (N-Ser) were prepared using a bead mill method. The mean particle size of the N-Ser was 110.3 nm at the time of preparation, and 148.1 nm one month later. The instillation of N-Ser had no effect on the amount of lacrimal fluid in normal rabbits (in vivo), but the MHN in N-Ser was found to expand the intercellular space in ex vivo rat corneas. In addition, the instillation of N-Ser increased the phosphorylation of Extracellular Signal-regulated Kinase (ERK)1/2, a factor involved in cell adhesion and cell proliferation in the corneal epithelium, in comparison with the instillation of sericin alone. The combination with MHN enhanced epithelial corneal wound healing by sericin in rat debrided corneal epithelium (in vivo). This study provides significant information to prepare potent drugs to cure severe keratopathy, such as diabetic keratopathy.
Nanogenerators as a Sustainable Power Source: State of Art, Applications, and Challenges
Authors: Sripadmanabhan Indira Sridhar , Aravind Vaithilingam Chockalingam , Oruganti Kameswara Satya Prakash , Mohd Faizal , Rahman Saidur
Published: May 2019

A sustainable power source to meet the needs of energy requirement is very much essential in modern society as the conventional sources are depleting. Bioenergy, hydropower, solar, and wind are some of the well-established renewable energy sources that help to attain the need for energy at mega to gigawatts power scale. Nanogenerators based on nano energy are the growing technology that facilitate self-powered systems, sensors, and flexible and portable electronics in the booming era of IoT (Internet of Things). The nanogenerators can harvest small-scale energy from the ambient nature and surroundings for efficient utilization. The nanogenerators were based on piezo, tribo, and pyroelectric effect, and the first of its kind was developed in the year 2006 by Wang et al. The invention of nanogenerators is a breakthrough in the field of ambient energy-harvesting techniques as they are lightweight, easily fabricated, sustainable, and care-free systems. In this paper, a comprehensive review on fundamentals, performance, recent developments, and application of nanogenerators in self-powered sensors, wind energy harvesting, blue energy harvesting, and its integration with solar photovoltaics are discussed. Finally, the outlook and challenges in the growth of this technology are also outlined.
MXene Boosted CoNi-ZIF-67 as Highly Efficient Electrocatalysts for Oxygen Evolution
Authors: Wen Yangyang , Wei Zhiting , Ma Chang , Xing Xiaofei , Li Zhenxing , Luo Dan
Published: May 2019

Oxygen evolution reaction (OER) is a pivotal step for many sustainable energy technologies, and exploring inexpensive and highly efficient electrocatalysts is one of the most crucial but challenging issues to overcome the sluggish kinetics and high overpotentials during OER. Among the numerous electrocatalysts, metal-organic frameworks (MOFs) have emerged as promising due to their high specific surface area, tunable porosity, and diversity of metal centers and functional groups. It is believed that combining MOFs with conductive nanostructures could significantly improve their catalytic activities. In this study, an MXene supported CoNi-ZIF-67 hybrid (CoNi-ZIF-67@Ti3C2Tx) was synthesized through the in-situ growth of bimetallic CoNi-ZIF-67 rhombic dodecahedrons on the Ti3C2Tx matrix via a coprecipitation reaction. It is revealed that the inclusion of the MXene matrix not only produces smaller CoNi-ZIF-67 particles, but also increases the average oxidation of Co/Ni elements, endowing the CoNi-ZIF-67@Ti3C2Tx as an excellent OER electrocatalyst. The effective synergy of the electrochemically active CoNi-ZIF-67 phase and highly conductive MXene support prompts the hybrid to process a superior OER catalytic activity with a low onset potential (275 mV vs. a reversible hydrogen electrode, RHE) and Tafel slope (65.1 mV∙dec−1), much better than the IrO2 catalysts and the pure CoNi-ZIF-67. This work may pave a new way for developing efficient non-precious metal catalyst materials.
Inflammatory Bowel Diseases and Food Additives: To Add Fuel on the Flames!
Authors: Marion-Letellier Rachel , Amamou Asma , Savoye Guillaume , Ghosh Subrata
Published: May 2019

Inflammatory bowel diseases (IBDs) develop in genetically predisposed individuals in response to environmental factors. IBDs are concomitant conditions of industrialized societies, and diet is a potential culprit. Consumption of ultra-processed food has increased over the last decade in industrialized countries, and epidemiological studies have found associations between ultra-processed food consumption and chronic diseases. Further studies are now required to identify the potential culprit in ultra-processed food, such as a poor nutritional composition or the presence of food additives. In our review, we will focus on food additives, i.e., substances from packaging in contact with food, and compounds formed during production, processing, and storage. A literature search using PubMed from inception to January 2019 was performed to identify relevant studies on diet and/or food additive and their role in IBDs. Manuscripts published in English from basic science, epidemiological studies, or clinical trials were selected and reviewed. We found numerous experimental studies highlighting the key role of food additives in IBD exacerbation but epidemiological studies on food additives on IBD risk are still limited. As diet is a modifiable environmental risk factor, this may offer a scientific rationale for providing dietary advice for IBD patients.