bluuebunny/crossref_metadata_2025 huggingface dataset:
Expanded Sampling Across Ontogeny in Deltasuchus motherali (Neosuchia, Crocodyliformes)
Authors: Drumheller Stephanie K. , Adams Thomas L. , Maddox Hannah , Noto Christopher R.
Published: April 2021

New material attributable to Deltasuchus motherali, a neosuchian from the Cenomanian of Texas, provides sampling across much of the ontogeny of this species. Detailed descriptions provide information about the paleobiology of this species, particularly with regards to how growth and development affected diet. Overall snout shape became progressively wider and more robust with age, suggesting that dietary shifts from juvenile to adult were not only a matter of size change, but of functional performance as well. These newly described elements provide additional characters upon which to base more robust phylogenetic analyses. The authors provide a revised diagnosis of this species, describing the new material and discussing incidents of apparent ontogenetic variation across the sampled population. The results of the ensuing phylogenetic analyses both situate Deltasuchus within an endemic clade of Appalachian crocodyliforms, separate and diagnosable from goniopholidids and pholidosaurs, herein referred to as Paluxysuchidae. This title is also available as Open Access on Cambridge Core.
Self-Representation of Marginalized Groups: A New Way of Thinking through W. E. B. Du Bois
Authors: Chowdhury Rashedur
Published: April 2021

I address an interesting puzzle of how marginalized groups gain self-representation and influence firms’ strategies. Accordingly, I examine the case of access to low-cost HIV/AIDS drugs in South Africa by integrating W. E. B. Du Bois’s work into stakeholder theory. Du Bois’s scholarly work, most notably his founding contribution to Black scholarship, has profound significance in the humanities and social sciences disciplines and vast potential to inspire a new way of thinking and doing research in the management and organization fields, including business ethics research. By drawing on Du Bois’s works, I argue that through reconstruction of their selves—knowing their souls—marginalized groups know their capabilities better, enabling them to overcome their political and strategic limitations and ensure their true self-representation. They are also empowered to use political imagination and strategies of resistance against more powerful opponents. This influences powerful actors to accept the demands of marginalized groups.
Ten Years After: From UN Guiding Principles to Multi-Fiduciary Obligations
Authors: RUGGIE John Gerard , REES Caroline , DAVIS Rachel
Published: May 2021

For the first time in four decades, leading business associations, corporations, and the corporate law and governance community are seriously debating moving beyond shareholder primacy toward some form of ‘stakeholder governance. But the how question unveils significant differences of opinion as well as difficulties. We focus on a pathway that complements the ambition of stakeholder governance, but which current reform proposals have largely overlooked. We draw on practical experience in the field of business and human rights, where leading companies are increasingly embedding human rights due diligence processes into their strategic decision-making. We contend that as human rights due diligence is made mandatory for companies, which it is in a growing number of jurisdictions, including for foreign firms with a significant business presence in them, risks to stakeholders become a material corporate governance issue. That makes it necessary for firms to address stakeholder concerns and to demonstrate that they are, with possible legal consequences for having failed to do where harm occurs. Such changes by themselves may not constitute a full-blown system of multi-fiduciary obligations, but they mark substantial strides on the path toward it, and they are doing it in the relatively near-term.
Bearing Witness: Creating the Conditions of Justice for First Nations Children
Authors: Ariss Rachel
Published: April 2021

In 2016, the Canadian Human Rights Tribunal found that Canada’s management of child welfare discriminates against First Nations children. The First Nations Child and Family Caring Society, one of the complainants, maintains a web-based campaign called “I Am A Witness,” providing details on the hearings and legal materials and asking visitors to act towards ending discrimination against First Nations children. What does it mean to bear witness to such discrimination? The concept of “witnessing” circulates through Indigenous oral traditions, communication and media theories, and the common law. This article explores the I Am A Witness campaign, arguing that as it evokes various theories of witnessing and builds public awareness of legal processes, it shifts spaces of and perspectives on legality beyond Western categories, creating a public that is enabled to bear witness to, and respond to, ongoing injustices against Indigenous peoples.
Increasing face-mask compliance among healthcare personnel during the coronavirus disease 2019 (COVID-19) pandemic
Authors: Datta Rupak , Glenn Keith , Pellegrino Anthony , Tuan Jessica , Linde Brian , Kayani Jehanzeb , Patel Kavin , Calo Lisbeysi , Dembry Louise Marie , Fisher Ann
Published: May 2021

Objective:

Prior studies of universal masking have not measured face-mask compliance. We performed a quality improvement study to monitor and improve face-mask compliance among healthcare personnel (HCP) during the coronavirus disease 2019 (COVID-19) pandemic.

Design:

Mixed-methods study.

Setting:

Tertiary-care center in West Haven, Connecticut.

Patients:

HCP including physicians, nurses, and ancillary staff.

Methods:

Face-mask compliance was measured through direct observations during a 4-week baseline period after universal masking was mandated. Frontline and management HCP completed semistructured interviews from which a multimodal intervention was developed. Direct observations were repeated during a 14-week period following implementation of the multimodal intervention. Differences between units were evaluated with χ2 testing using the Bonferroni correction. Face-mask compliance between baseline and intervention periods was compared using time-series regression.

Results:

Among 1,561 observations during the baseline period, median weekly face-mask compliance was 82.2% (range, 80.8%–84.4%). Semistructured interviews were performed with 16 HCP. Qualitative analysis informed the development of a multimodal intervention consisting of audit and passive feedback, active discussion, and increased communication from leadership. Among 2,651 observations during the intervention period, median weekly face-mask compliance was 92.6% (range, 84.6%–97.9%). There was no difference in weekly face-mask compliance between COVID-19 and non–COVID-19 units. The multimodal intervention was associated with an increase in face-mask compliance (β = 0.023; P = .002).

Conclusions:

Face-mask compliance remained suboptimal among HCP despite a facility-wide mandate for universal masking. A multimodal intervention consisting of audit and passive feedback, active discussion, and increased communication from leadership was effective in increasing face-mask compliance among HCP.
Assessing vulnerability and resistance to plant invasions: a native community perspective
Authors: Ibáñez Inés , Liu Gang , Petri Laís , Schaffer-Morrison Sam , Schueller Sheila
Published: May 2021

Risk assessments of biological invasions rarely account for native species performance and community features, but the assessment presented here could provide additional insights for management aimed at decreasing vulnerability or increasing resistance of a plant community to invasions. To gather information on the drivers of native plant communities’ vulnerability and resistance to invasion, we conducted a literature search and meta-analysis. Using the data we collected, we compared native and invasive plant performance between sites with high and low levels of invasion. We then investigated conditions under which native performance increased, decreased, or did not change with respect to invasive plants. We analyzed data from 214 publications summing to 506 observations. There were six main drivers of vulnerability to invasion: disturbance, decrease in resources, increase in resources, lack of biotic resistance, lack of natural enemies, and differences in propagule availability between native and invasive species. The two mechanisms of vulnerability to invasion associated with a strong decline in native plant performance were propagule availability and lack of biotic resistance. Native plants marginally benefited from enemy release and from decreases in resources, while invasive plants strongly benefited from both increased resources and lack of enemies. Fluctuation of resources, decreases and increases, were strongly associated with higher invasive performance, while native plants varied in their responses. These differences were particularly strong in instances of decreasing water or nutrients and of increasing light and nutrients. We found overall neutral to positive responses of native plant communities to disturbance, but natives were outperformed by invasive species when disturbance was caused by human activities. We identified ecosystem features associated with both vulnerability and resistance to invasion, then used our results to inform management aimed at protecting the native community.
SAME-SEX RELATIONS AND LEGAL TRADITIONS IN CAMEROON AND SOUTH AFRICA
Authors: Bongmba Elias Kifon
Published: April 2021

This essay revisits the debates and legal contests that grew in Cameroon at the turn of the millennium but failed to bring justice for members of the lesbian, gay, bisexual, transgender, and queer (LGBTQ) community. Several members of sexual minorities were tried in Cameroon courts and sentenced to serve jail time. In order to reflect on the state of legal limbo for LGBTQ people in Cameroon, I also revisit the South African caseMinister of Home Affairs and the Director General of Home Affairs versus Marie Adrianna Fourie and Cecelia Johanna Bonthuys, which led to legalization of same-sex relationships and marriage in South Africa. In the first and longer part of the essay, I discuss the situation in Cameroon and South Africa. In the second part, I briefly discuss the different legal outcomes in the two countries. I conclude with a brief discussion of signs of hope in the critical dialogue on justice in the debate on same-sex relations in Africa. My goal in this essay is not to offer expert opinion on the legal entanglements on the question of same-sex relations, but to demonstrate that legal and constitutional protections offer the best chance for gaining the rights of LGBTQ people in Africa.
Physical limits to meltwater penetration in firn
Authors: Humphrey Neil F. , Harper Joel T. , Meierbachtol Toby W.
Published: April 2021

Processes governing meltwater penetration into cold firn remain poorly constrained. Here, in situ experiments are used to develop a grain-scale model to investigate physical limitations on meltwater infiltration in firn. At two sites in Greenland, drilling pumped water into cold firn to >75 m depth, and the thermo-hydrologic evolution of the firn column was measured. Rather than filling all available pore space, the water formed perched aquifers with downward penetration halted by thermal and density conditions. The two sites formed deep aquifers at ~40 m depth and at densities considerably less than the air pore close-off density (~725 kg m−3at −18°C, and ~750 kg m−3at −14°C), demonstrating that some pore space at depth remains inaccessible. A geometric grain-scale model of firn is constructed to quantify the limits of a descending fully saturated wetting front in cold firn. Agreement between the model and field data implies the model includes the first-order effects of water and heat flow in a firn lattice. The model constrains the relative importance of firn density, temperature and grain/pore size in inhibiting wetting front migration. Results imply that deep infiltration, including that which leads to firn aquifer formation, does not have access to all available firn pore space.
Democratizing the Party: The Effects of Primary Election Reforms in Ghana
Authors: Ichino Nahomi , Nathan Noah L.
Published: April 2021

The recent expansion of the primary electorate by one of Ghana's major parties offers a rare opportunity to assess the effects of franchise extensions in contemporary new democracies. Using an original dataset on candidate entry and nominations, this article shows that expanding the primary electorate opened paths to office for politicians from social groups that were previously excluded, such as women and ethnic groups outside the party's core national coalition. The authors propose that democratizing candidate selection has two consequences in patronage-oriented political systems: vote buying will become a less effective strategy and the electorate will become more diverse. These changes, in turn, affect the types of politicians who seek and win legislative nominations. This suggests that a simple shift in who votes in intraparty primaries can be a key institutional mechanism for improving the descriptive representation of women and other under-represented groups.
Echolocating the Social: Silence, Voice, and Affect in China's Hundred Flowers and Anti-Rightist Campaigns, 1956–58
Authors: Lekner Dayton
Published: April 2021

What is the catalytic element that brings about widespread participation in a mass campaign? Is it ideology? Self-interest? Emotional states of fear, hatred, or love? Taking into account the recent proliferation of sound studies approaches to the history of the People's Republic of China, this article explores this question through the sonic experience of the campaign. Previous studies of the soundscapes of the Mao era have focused upon state initiatives of sound-borne propaganda and their role in the transmission of revolutionary ideas. Using a case study of the Hundred Flowers and Anti-Rightist campaigns of 1956–58, I examine the reception of such propaganda with a focus on silence, sound, and voice and their affective qualities. Through the use of diaries, memoirs, contemporary newspapers, and interviews, I explore the extra-linguistic aspects of the campaign to ask what, outside of revolutionary words and emotions, brought the subjects of a campaign from silence to vocal participation.
Evaluating the technological properties of lactic acid bacteria in Wagyu cattle milk
Authors: Tsuda Harutoshi , Kodama Kana
Published: May 2021

This paper reveals the technological properties of lactic acid bacteria isolated from raw milk (colostrum and mature milk) of Wagyu cattle raised in Okayama Prefecture, Japan. Isolates were identified based on their physiological and biochemical characteristics as well as 16S rDNA sequence analysis. Streptococcus lutetiensis and Lactobacillus plantarum showed high acid and diacetyl-acetoin production in milk after 24 h of incubation at 40 and 30°C, respectively. These strains are thought to have potential for use as starter cultures and adjunct cultures for fermented dairy products.
Retinal microglia polarization in diabetic retinopathy
Authors: Li Xin , Yu Zi-Wei , Li Hui-Yao , Yuan Yue , Gao Xin-Yuan , Kuang Hong-Yu
Published: May 2021

Microglia, the main immune cell of the central nervous system (CNS), categorized into M1-like phenotype and M2-like phenotype, play important roles in phagocytosis, cell migration, antigen presentation, and cytokine production. As a part of CNS, retinal microglial cells (RMC) play an important role in retinal diseases. Diabetic retinopathy (DR) is one of the most common complications of diabetes. Recent studies have demonstrated that DR is not only a microvascular disease but also retinal neurodegeneration. RMC was regarded as a central role in neurodegeneration and neuroinflammation. Therefore, in this review, we will discuss RMC polarization and its possible regulatory factors in early DR, which will provide new targets and insights for early intervention of DR.
Antioxidants in smokers
Authors: Astori Emanuela , Garavaglia Maria L. , Colombo Graziano , Landoni Lucia , Portinaro Nicola M. , Milzani Aldo , Dalle-Donne Isabella
Published: April 2021

Cigarette smoke (CS) is likely the most common preventable cause of human morbidity and mortality worldwide. Consequently, inexpensive interventional strategies for preventing CS-related diseases would positively impact health systems. Inhaled CS is a powerful inflammatory stimulus and produces a shift in the normal balance between antioxidants and oxidants, inducing oxidative stress in both the respiratory system and throughout the body. This enduring and systemic pro-oxidative state within the body is reflected by increased levels of oxidative stress and inflammation biomarkers seen in smokers. Smokers might benefit from consuming antioxidant supplements, or a diet rich in fruit and vegetables, which can reduce the CS-related oxidative stress. This review provides an overview of the plasma profile of antioxidants observable in smokers and examines the heterogeneous literature to elucidate and discuss the effectiveness of interventional strategies based on antioxidant supplements or an antioxidant-rich diet to improve the health of smokers. An antioxidant-rich diet can provide an easy-to-implement and cost-effective preventative strategy to reduce the risk of CS-related diseases, thus being one of the simplest ways for smokers to stay in good health for as long as possible. The health benefits attributable to the intake of antioxidants have been observed predominantly when these have been consumed within their natural food matrices in an optimal antioxidant-rich diet, while these preventive effects are rarely achieved with the intake of individual antioxidants, even at high doses.
Negative parental emotional environment increases the association between childhood behavioral problems and impaired recognition of negative facial expressions
Authors: Burley Daniel T. , Hobson Christopher W. , Adegboye Dolapo , Shelton Katherine H. , van Goozen Stephanie H.M.
Published: April 2021

Impaired facial emotion recognition is a transdiagnostic risk factor for a range of psychiatric disorders. Childhood behavioral difficulties and parental emotional environment have been independently associated with impaired emotion recognition; however, no study has examined the contribution of these factors in conjunction. We measured recognition of negative (sad, fear, anger), neutral, and happy facial expressions in 135 children aged 5–7 years referred by their teachers for behavioral problems. Parental emotional environment was assessed for parental expressed emotion (EE) – characterized by negative comments, reduced positive comments, low warmth, and negativity towards their child – using the 5-minute speech sample. Child behavioral problems were measured using the teacher-informant Strengths and Difficulties Questionnaire (SDQ). Child behavioral problems and parental EE were independently associated with impaired recognition of negative facial expressions specifically. An interactive effect revealed that the combination of both factors was associated with the greatest risk for impaired recognition of negative faces, and in particular sad facial expressions. No relationships emerged for the identification of happy facial expressions. This study furthers our understanding of multidimensional processes associated with the development of facial emotion recognition and supports the importance of early interventions that target this domain.
STATE-DEPENDENT FORMATION OF INFLATION EXPECTATIONS
Authors: Gwak Bopjun
Published: April 2021

The learning-from-experience model of Malmendier and Nagel [(2016) Quarterly Journal of Economics 131, 53–87] successfully reproduces the heterogeneity in inflation expectations across age groups. However, inflation expectations presumably depend on not only age and experience but also inflation regime. Therefore, this paper proposes an extended learning-from-experience model, in which expectation formation depends on inflation regime. Estimating the model with US data, I find that when inflation is higher and more volatile, households place more weight on recent data when making private forecasts. Moreover, in this regime, households rely more heavily on private forecasts than on public information.
Identifying opportunities for strengthening advice to enhance vegetable liking in the early years of life: qualitative consensus and triangulation methods
Authors: Bell Lucinda K , Gardner Claire , Kumar Saravana , Wong Hoi Y , Johnson Brittany , Byrne Rebecca , Campbell Karen J , Liem Djin Gie , Russell Catherine (Georgie) , Denney-Wilson Elizabeth , Netting Merryn , Bishop Lola , Cox David N , Poelman Astrid AAM , Arguelles Jennifer , Golley Rebecca K
Published: May 2021

Objective:

To prioritise and refine a set of evidence-informed statements into advice messages to promote vegetable liking in early childhood, and to determine applicability for dissemination of advice to relevant audiences.

Design:

A nominal group technique (NGT) workshop and a Delphi survey were conducted to prioritise and achieve consensus (≥70 % agreement) on thirty evidence-informed maternal (perinatal and lactation stage), infant (complementary feeding stage) and early years (family diet stage) vegetable-related advice messages. Messages were validated via triangulation analysis against the strength of evidence from an Umbrella review of strategies to increase children’s vegetable liking, and gaps in advice from a Desktop review of vegetable feeding advice.

Setting:

Australia.

Participants:

A purposeful sample of key stakeholders (NGT workshop,n8 experts; Delphi survey,n23 end users).

Results:

Participant consensus identified the most highly ranked priority messages associated with the strategies of:‘in-utero exposure’(perinatal and lactation,n56 points) and‘vegetable variety’(complementary feeding,n97 points; family diet,n139 points). Triangulation revealed two strategies (‘repeated exposure’ and ‘variety’) and their associated advice messages suitable for policy and practice, twelve for research and four for food industry.

Conclusions:

Supported by national and state feeding guideline documents and resources, the advice messages relating to ‘repeated exposure’ and ‘variety’ to increase vegetable liking can be communicated to families and caregivers by healthcare practitioners. The food industry provides a vehicle for advice promotion and product development. Further research, where stronger evidence is needed, could further inform strategies for policy and practice, and food industry application.
Othering in Media Representations of Elderly Care: Using the Social Justice Framework to Make Sense of Public Discourses on Migrants and Culture
Authors: Lindblom Jonas , Torres Sandra
Published: April 2021

Population ageing and international migration are two of the major societal trends challenging European elderly care regimes at present. Virtually no research has addressed how public discourses about the implications of these trends for elderly care are shaped in different countries. This article addresses this knowledge gap, examining how Swedish daily newspaper (SvD and DN) reporting on elderly care between 1995 and 2017 (N=370) depicts the impact of increased ethno-cultural diversity on this sector. Through content analysis, this article brings attention to the representations of migrants and culture that this reporting has deployed, and the rhetorical practices that the reporting has relied on (i.e. genre stratification, hegemonisation, homogenisation, normative referencing and idealisation/ diminishment). The article exposes how the ‘Othering’ of migrants is accomplished in Sweden’s daily newspaper reporting on elderly care, and problematizes the ethea of inclusiveness and equality of care with which we have come to associate this welfare sector.
Evidence on the Conceptual Distinctness of Normal Grief From Depression
Authors: Wittkowski Joachim , Scheuchenpflug Rainer
Published: May 2021

Abstract. Background: The distinctness of grief from depression has been the subject of a long scholarly debate, even influencing definitions of diagnostic criteria. Aims: This study aims at clarifying the issue by a multifaceted analysis of data from a large German sample. Method: A community sample of 406 bereaved persons answered the Wuerzburg Grief Inventory (WGI), a multidimensional grief questionnaire designed to measure normal grief in the German language, and the General Depression Scale – Short Version (GDS-S), a self-report depression scale. Data were analyzed by factor analysis to identify structural (dis-)similarities of the constructs, and analysis of variance (ANOVA) to identify the influence of the factors relationship to the deceased, type of death, and time since loss on grief measures and depression scores. Results: Factor analysis clustered items referring to grief-related impairments and depression into one factor, items referring to other dimensions of grief on separate factors, however. Relationship to the deceased influenced the grief measures impairments and nearness to the deceased, but not depression scores if controlled for impairments. Type of death showed specific effects on grief scores, but not on depression scores. Time since loss influenced grief scores, but not depression scores. Limitations: The analysis is based on a self-selected community sample of grieving persons, self-report measures, and in part, on cross-sectional data. Conclusion: Factor analysis and objective data show a clear distinction of dimensions of grief and depression. The human experience of grief contains a sense of nearness to the lost person, feelings of guilt, and positive aspects of the loss experience in addition to components resembling depression.
Combined Effect of the PDO and ENSO on the Date of the First Tropical Cyclone Landfall in Continental East Asia
Authors: Li Shuang , Xiao Ziniu , Zhao Yuchun
Published: May 2021

The date of the first tropical cyclone (TC) landfall (DFTCL) is important for TC disaster prevention. This study analyzes the variation in the characteristics of the DFTCL in continental East Asia and investigates the possible effect of the Pacific Decadal Oscillation (PDO) and the El Niño‐Southern Oscillation (ENSO) on the DFTCL. Results show that the combined effect of the PDO and ENSO plays an important role in whether TC landfall occurs relatively earlier or later. During negative PDO phases, the DFTCL is impacted significantly by ENSO events. TCs tend to make landfall later (earlier) in the years following El Niño (La Niña). However, little influence is noticeable for the ENSO events during positive PDO phases. The combined effect is possibly associated with the anomalous anticyclone (cyclone) in the western North Pacific (South China Sea) which is modulated by different PDO phases asymmetrically. During negative PDO phases, the tropical Indian Ocean (TIO) has a strong response to El Niño events in winter. The warm anomaly persists in the following months until early summer. This induces the anomalous anticyclone in the western North Pacific which is enhanced by the anomalous meridional circulation. The anomalous anticyclone can suppress TC genesis and landfall. On the other hand, the TIO has a significant cooling response to La Niña events and induces the anomalous cyclone near the South China Sea. The anomalous cyclone favors earlier than usual TC genesis and landfall. However, this relationship cannot be detected in the positive PDO phase.
Meridional Migration of Eastern North Pacific Tropical Cyclogenesis: Joint Contribution of Interhemispheric Temperature Differential and ENSO
Authors: Zhao Haikun , Zhao Kai , Cao Jian , Klotzbach Philip J. , Raga Graciela B. , Ma Zhanhong
Published: May 2021

This study finds that the meridional migration of tropical cyclogenesis (TCG) between June and November over the eastern North Pacific is mainly due to changes in the Hadley circulation and associated changes in the location of the intertropical convergence zone as modulated by ENSO and the interhemispheric temperature differential (ITD). These two factors are largely independent of each other and play significant and nearly equivalent roles in the meridional migration of TCG over the ENP from 1979 to 2018. Through the use of regression models, we find that the combination of these two factors has significant skill in capturing annual TCG meridional distribution over the ENP basin. June–October ENSO, as defined by Niño‐3.4, can be skillfully predicted with ∼1 season lead time, while ITD can be skillfully predicted ∼3 seasons in advance. This predictability allows for the potential for seasonal outlooks of TCG meridional movement over the ENP. The influence of ITD on TCG meridional migration over the ENP has repercussions on climate change timescales as well, due to the potential TCG meridional migration over the ENP in response to global warming. Under the Shared Socioeconomic Pathways 2–4.5 scenario, the ITD shows a robust increase among the 23 models of version 6 of the Coupled Model Intercomparison Project. However, the considerable uncertainty in the model response of ENSO to global warming reduces confidence in projections of TCG meridional migration over the ENP.
Constructing Shapes and Mixing Structures of Black Carbon Particles With Applications to Optical Calculations
Authors: Wang Yuanyuan , Pang Yuner , Huang Jin , Bi Lei , Che Huizheng , Zhang Xiaoye , Li Weijun
Published: May 2021

Black carbon (BC) aerosols strongly absorb the solar radiation, affecting the regional and global climate through direct and indirect radiative forcing. The optical properties of BC are critical factors to estimate their radiative forcing. However, the optical absorption of BC is still under controversy partially due to the weakness in quantifying their complex morphology and mixing structures. Although a Discrete Dipole Approximation (DDA) can calculate optical properties of fine particles with arbitrary shapes, an appropriate definition of realistic BC shape models for optical simulation is essentially required. Here we present a novel Electron‐Microscope‐to‐BC‐Simulation (EMBS) tool to construct realistic BC shape models with various morphology and mixing structures for optical calculation using DDA. The optical properties of BC particles with different particle morphology, coating thickness, and embedded fraction (F) are estimated based on electron microscope. We find that absorption enhancement (Eabs) of the realistic irregular model is larger than that of the present commonly used spherical model (i.e., BC aggregate with spherical coating). The BC core morphology greatly influences Eabs of the embedded BC particles with irregular coating when the volume‐equivalent‐diameter ratio of particle to core (Dp/Dc) is larger than 1.8. The F significantly influences Eabs of BC particles, suggesting that the mixing structure between coating and core is an important factor to determine the optical absorption of aged BC particles. The study highlights that the BC core morphology, coating shape, coating thickness, and mixing structures influence their optical properties and should be considered as important variables in climate models.
Symptomatic and preventive effects of the novel phosphodiesterase-9 inhibitor BI 409306 in an immune-mediated model of neurodevelopmental disorders
Authors: Scarborough Joseph , Mattei Daniele , Dorner-Ciossek Cornelia , Sand Michael , Arban Roberto , Rosenbrock Holger , Richetto Juliet , Meyer Urs
Published: May 2021

BI 409306, a phosphodiesterase-9 inhibitor under development for treatment of schizophrenia and attenuated psychosis syndrome (APS), promotes synaptic plasticity and cognition. Here, we explored the effects of BI 409306 treatment in the polyriboinosinic-polyribocytidilic acid (poly[I:C])-based mouse model of maternal immune activation (MIA), which is relevant to schizophrenia and APS. In Study 1, adult offspring received BI 409306 0.2, 0.5, or 1 mg/kg or vehicle to establish an active dose. In Study 2, adult offspring received BI 409306 1 mg/kg and/or risperidone 0.025 mg/kg, risperidone 0.05 mg/kg, or vehicle, to evaluate BI 409306 as add-on to standard therapy for schizophrenia. In Study 3, offspring received BI 409306 1 mg/kg during adolescence only, or continually into adulthood to evaluate preventive effects of BI 409306. We found that BI 409306 significantly mitigated MIA-induced social interaction deficits and amphetamine-induced hyperlocomotion, but not prepulse inhibition impairments, in a dose-dependent manner (Study 1). Furthermore, BI 409306 1 mg/kg alone or in combination with risperidone 0.025 mg/kg significantly reversed social interaction deficits and attenuated amphetamine-induced hyperlocomotion in MIA offspring (Study 2). Finally, we revealed that BI 409306 1 mg/kg treatment restricted to adolescence prevented adult deficits in social interaction, whereas continued treatment into adulthood also significantly reduced amphetamine-induced hyperlocomotion (Study 3). Taken together, our findings suggest that symptomatic treatment with BI 409306 can restore social interaction deficits and dopaminergic dysfunctions in a MIA model of neurodevelopmental disruption, lending preclinical support to current clinical trials of BI 409306 in patients with schizophrenia. Moreover, BI 409306 given during adolescence has preventive effects on adult social interaction deficits in this model, supporting its use in people with APS.
Microbial iron and carbon metabolism as revealed by taxonomy-specific functional diversity in the Southern Ocean
Authors: Sun Ying , Debeljak Pavla , Obernosterer Ingrid
Published: May 2021

Marine microbes are major drivers of all elemental cycles. The processing of organic carbon by heterotrophic prokaryotes is tightly coupled to the availability of the trace element iron in large regions of the Southern Ocean. However, the functional diversity in iron and carbon metabolism within diverse communities remains a major unresolved issue. Using novel Southern Ocean meta-omics resources including 133 metagenome-assembled genomes (MAGs), we show a mosaic of taxonomy-specific ecological strategies in naturally iron-fertilized and high nutrient low chlorophyll (HNLC) waters. Taxonomic profiling revealed apparent community shifts across contrasting nutrient regimes. Community-level and genome-resolved metatranscriptomics evidenced a moderate association between taxonomic affiliations and iron and carbon-related functional roles. Diverse ecological strategies emerged when considering the central metabolic pathways of individual MAGs. Closely related lineages appear to adapt to distinct ecological niches, based on their distribution and gene regulation patterns. Our in-depth observations emphasize the complex interplay between the genetic repertoire of individual taxa and their environment and how this shapes prokaryotic responses to iron and organic carbon availability in the Southern Ocean.
Metabolic flexibility allows bacterial habitat generalists to become dominant in a frequently disturbed ecosystem
Authors: Chen Ya-Jou , Leung Pok Man , Wood Jennifer L , Bay Sean K , Hugenholtz Philip , Kessler Adam J , Shelley Guy , Waite David W , Franks Ashley E , Cook Perran L M , Greening Chris
Published: May 2021

Ecological theory suggests that habitat disturbance differentially influences distributions of habitat generalist and specialist species. While well-established for macroorganisms, this theory has rarely been explored for microorganisms. Here we tested these principles in permeable (sandy) sediments, ecosystems with much spatiotemporal variation in resource availability and physicochemical conditions. Microbial community composition and function were profiled in intertidal and subtidal sediments using 16S rRNA gene amplicon sequencing and metagenomics, yielding 135 metagenome-assembled genomes. Community composition and metabolic traits modestly varied with sediment depth and sampling date. Several taxa were highly abundant and prevalent in all samples, including within the orders Woeseiales and Flavobacteriales, and classified as habitat generalists; genome reconstructions indicate these taxa are highly metabolically flexible facultative anaerobes and adapt to resource variability by using different electron donors and acceptors. In contrast, obligately anaerobic taxa such as sulfate reducers and candidate lineage MBNT15 were less abundant overall and only thrived in more stable deeper sediments. We substantiated these findings by measuring three metabolic processes in these sediments; whereas the habitat generalist-associated processes of sulfide oxidation and fermentation occurred rapidly at all depths, the specialist-associated process of sulfate reduction was restricted to deeper sediments. A manipulative experiment also confirmed habitat generalists outcompete specialist taxa during simulated habitat disturbance. Together, these findings show metabolically flexible habitat generalists become dominant in highly dynamic environments, whereas metabolically constrained specialists are restricted to narrower niches. Thus, an ecological theory describing distribution patterns for macroorganisms likely extends to microorganisms. Such findings have broad ecological and biogeochemical ramifications.
Association of co-occurring opioid or other substance use disorders with increased healthcare utilization in patients with depression
Authors: Vekaria Veer , Bose Budhaditya , Murphy Sean M. , Avery Jonathan , Alexopoulos George , Pathak Jyotishman
Published: May 2021

Substance use disorders (SUDs) commonly co-occur with mental illness. However, the ongoing addiction crisis raises the question of how opioid use disorder (OUD) impacts healthcare utilization relative to other SUDs. This study examines the utilization patterns of patients with major depressive disorder (MDD) and: (1) co-occurring OUD (MDD-OUD); (2) a co-occurring SUD other than OUD (MDD-NOUD); and (3) no co-occurring SUD (MDD-NSUD). We analyzed electronic health records (EHRs) derived from multiple health systems across the New York City (NYC) metropolitan area between January 2008 and December 2017. 11,275 patients aged ≥18 years with a gap of 30–180 days between 2 consecutive MDD diagnoses and an antidepressant prescribed 0–180 days after any MDD diagnosis were selected, and prevalence of any SUD was 24%. Individuals were stratified into comparison groups and matched on age, gender, and select underlying comorbidities. Prevalence rates and encounter frequencies were measured and compared across outpatient, inpatient, and emergency department (ED) settings. Our key findings showed that relative to other co-occurring SUDs, OUD was associated with larger increases in the rates and odds of using substance-use-related services in all settings, as well as services that integrate mental health and substance abuse treatments in inpatient and ED settings. OUD was also associated with larger increases in total encounters across all settings. These findings and our proposed policy recommendations could inform efforts towards targeted OUD interventions, particularly for individuals with underlying mental illness whose treatment and recovery are often more challenging.
Vitamin B6 deficiency hyperactivates the noradrenergic system, leading to social deficits and cognitive impairment
Authors: Toriumi Kazuya , Miyashita Mitsuhiro , Suzuki Kazuhiro , Yamasaki Nao , Yasumura Misako , Horiuchi Yasue , Yoshikawa Akane , Asakura Mai , Usui Noriyoshi , Itokawa Masanari , Arai Makoto
Published: May 2021

We have reported that a subpopulation of patients with schizophrenia have lower levels of vitamin B6 (VB6) in peripheral blood than do healthy controls. In a previous study, we found that VB6 level was inversely proportional to the patient’s positive and negative symptom scale (PANSS) score for measuring symptom severity, suggesting that the loss of VB6 might contribute to the development of schizophrenia symptoms. In the present study, to clarify the relationship between VB6 deficiency and schizophrenia, we generated VB6-deficient (VB6(−)) mice through feeding with a VB6-lacking diet as a mouse model for the subpopulation of schizophrenia patients with VB6 deficiency. After feeding for 4 weeks, plasma VB6 level in VB6(−) mice decreased to 3% of that in control mice. The VB6(−) mice showed social deficits and cognitive impairment. Furthermore, the VB6(−) mice showed a marked increase in 3-methoxy-4-hydroxyphenylglycol (MHPG) in the brain, suggesting enhanced noradrenaline (NA) metabolism in VB6(−) mice. We confirmed the increased NA release in the prefrontal cortex (PFC) and the striatum (STR) of VB6(−) mice through in vivo microdialysis. Moreover, inhibiting the excessive NA release by treatment with VB6 supplementation into the brain and α2A adrenoreceptor agonist guanfacine (GFC) suppressed the increased NA metabolism and ameliorated the behavioral deficits. These findings suggest that the behavioral deficits shown in VB6(−) mice are caused by enhancement of the noradrenergic (NAergic) system.
The hypoxia sensitive metal transcription factor MTF-1 activates NCX1 brain promoter and participates in remote postconditioning neuroprotection in stroke
Authors: Valsecchi Valeria , Laudati Giusy , Cuomo Ornella , Sirabella Rossana , Del Prete Annalisa , Annunziato Lucio , Pignataro Giuseppe
Published: April 2021

Remote limb ischemic postconditioning (RLIP) is an experimental strategy in which short femoral artery ischemia reduces brain damage induced by a previous harmful ischemic insult. Ionic homeostasis maintenance in the CNS seems to play a relevant role in mediating RLIP neuroprotection and among the effectors, the sodium-calcium exchanger 1 (NCX1) may give an important contribution, being expressed in all CNS cells involved in brain ischemic pathophysiology. The aim of this work was to investigate whether the metal responsive transcription factor 1 (MTF-1), an important hypoxia sensitive transcription factor, may (i) interact and regulate NCX1, and (ii) play a role in the neuroprotective effect mediated by RLIP through NCX1 activation. Here we demonstrated that in brain ischemia induced by transient middle cerebral occlusion (tMCAO), MTF-1 is triggered by a subsequent temporary femoral artery occlusion (FAO) and represents a mediator of endogenous neuroprotection. More importantly, we showed that MTF-1 translocates to the nucleus where it binds the metal responsive element (MRE) located at −23/−17 bp of Ncx1 brain promoter thus activating its transcription and inducing an upregulation of NCX1 that has been demonstrated to be neuroprotective. Furthermore, RLIP restored MTF-1 and NCX1 protein levels in the ischemic rat brain cortex and the silencing of MTF-1 prevented the increase of NCX1 observed in RLIP protected rats, thus demonstrating a direct regulation of NCX1 by MTF-1 in the ischemic cortex of rat exposed to tMCAO followed by FAO. Moreover, silencing of MTF-1 significantly reduced the neuroprotective effect elicited by RLIP as demonstrated by the enlargement of brain infarct volume observed in rats subjected to RLIP and treated with MTF-1 silencing. Overall, MTF-dependent activation of NCX1 and their upregulation elicited by RLIP, besides unraveling a new molecular pathway of neuroprotection during brain ischemia, might represent an additional mechanism to intervene in stroke pathophysiology.
ITIH5, a p53-responsive gene, inhibits the growth and metastasis of melanoma cells by downregulating the transcriptional activity of KLF4
Authors: Liu Jia , Cao Feng , Li Xiaojie , Zhang Li , Liu Zhengrong , Li Xiaodong , Lin Jingrong , Han Chuanchun
Published: May 2021

ITIH5, a member of the inter-α-trypsin inhibitory (ITI) gene family, acts as a putative tumour-suppressor gene in many cancers. However, its role and the regulatory mechanism in melanoma are still unclear. Here, we found that the expression of ITIH5 was decreased in melanoma tissues compared with normal skin tissues. Decreased expression of ITIH5 was correlated with clinicopathological features and predicted poor prognosis in patients with melanoma. Forced expression of ITIH5 significantly inhibited melanoma cell proliferation and metastasis in vitro and ex vivo while knockdown of ITIH5 expression enhanced the malignant behaviour of melanoma cells. In further mechanistic studies, we showed that p53 can directly bind to the promoter of ITIH5 and thus promotes transcription of ITIH5 in melanoma cells. Additionally, we found that ITIH5 interacted with Krüppel-like factor 4 (KLF4) and inhibited its transcriptional activity. Collectively, our data not only identified a tumour-suppressive role of ITIH5 in melanoma but also revealed that upregulation of ITIH5 by p53 suppressed melanoma cell growth and migration likely by downmodulating the transcriptional activity of KLF4.
A novel Lnc408 maintains breast cancer stem cell stemness by recruiting SP3 to suppress CBY1 transcription and increasing nuclear β-catenin levels
Authors: Wen Siyang , Qin Yilu , Wang Rui , Yang Liping , Zeng Huan , Zhu Pengpeng , Li Qiao , Qiu Yuxiang , Chen Shanchun , Liu Yongcan , Hou Yixuan , Tang Xi , Liu Manran , Tu Gang
Published: May 2021

Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). The complicated mechanisms underlying the maintenance of CSCs are keeping in illustration. Long noncoding RNAs (lncRNAs), due to their multifunction in various biological processes, have been indicated to play a crucial role in CSC renewal and stemness maintenance. Using lncRNA array, we identified a novel lncRNA (named lnc408) in epithelial–mesenchymal transition-related breast CSCs (BCSCs). The lnc408 is high expressed in BCSCs in vitro and in vivo. The enhanced lnc408 is critical to BCSC characteristics and tumorigenesis. Lnc408 can recruit transcript factor SP3 to CBY1 promoter to serve as an inhibitor in CBY1 transcription in BCSCs. The high expressed CBY1 in non-BCSC interacts with 14-3-3 and β-catenin to form a ternary complex, which leads a translocation of the ternary complex into cytoplasm from nucleus and degradation of β-catenin in phosphorylation-dependent pattern. The lnc408-mediated decrease of CBY1 in BCSCs impairs the formation of 14-3-3/β-catenin/CBY1 complex, and keeps β-catenin in nucleus to promote CSC-associated CD44, SOX2, Nanog, Klf4, and c-Myc expressions and contributes to mammosphere formation; however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. In shortly, these findings highlight that the novel lnc408 functions as an oncogenic factor by recruiting SP3 to inhibit CBY1 expression and β-catenin accumulation in nucleus to maintain stemness properties of BCSCs. Lnc408–CBY1–β-catenin signaling axis might serve as a new diagnostic and therapeutic target for breast cancer.