bluuebunny/crossref_metadata_2025 huggingface dataset:
Unusual Giant Syringomyelia with Neurocutaneous Melanosis
Authors: Shrestha R , Cohen AR
Published: April 2015

A two year old male presented with feeding intolerance and lethargy. Mother had normal delivery with term baby. The patient denied previous trauma or recent weight loss. Physical examination revealed neurocutaneous melanosis (NCM) over lower back side of body and no signs of peripheral motor deficit or abnormal reflexes. Hematological investigations showed no abnormality. Magnetic resonance (MR) imaging found a large, cystic cavity that was eccentric in the cervicothoracic spine extending from C3 to L1. Suboccipital decompressive craniectomy, bilateral C1 laminectomy, and occipital duraplasty were done. J Nepal Paediatr Soc 2014;34(3):250-251 DOI: http://dx.doi.org/10.3126/jnps.v34i3.11165
Exploring the composition of the searchable web: a corpus-based taxonomy of web registers
Authors: Biber Douglas , Egbert Jesse , Davies Mark
Published: April 2015

One major challenge for Web-As-Corpus research is that a typical Web search provides little information about the register of the documents that are searched. Previous research has attempted to address this problem (e.g., through the Automatic Genre Identification initiative), but with only limited success. As a result, we currently know surprisingly little about the distribution of registers on the web.

In this study, we tackle this problem through a bottom-up user-based investigation of a large, representative corpus of web documents. We base our investigation on a much larger corpus than those used in previous research (48,571 web documents), and obtained through random sampling from across the full range of documents that are publically available on the searchable web. Instead of relying on individual expert coders, we recruit typical end-users of the Web for register coding, with each document in the corpus coded by four different raters. End-users identify basic situational characteristics of each web document, coded in a hierarchical manner. Those situational characteristics lead to general register categories, which eventually lead to lists of specific sub-registers. By working through a hierarchical decision tree, users are able to identify the register category of most Internet texts with a high degree of reliability.

After summarising our methodological approach, this paper documents the register composition of the searchable web. Narrative registers are found to be the most prevalent, while Opinion and Informational Description/Explanation registers are also found to be extremely common. One of the major innovations of the approach adopted here is that it permits an empirical identification of ‘hybrid’ documents, which integrate characteristics from multiple general register categories (e.g., opinionated-narrative). These patterns are described and illustrated through sample Internet documents.
A Study on the Fuzzy-Logic-Based Solar Power MPPT Algorithms Using Different Fuzzy Input Variables
Authors: Shiau Jaw-Kuen , Wei Yu-Chen , Chen Bo-Chih
Published: April 2015

Maximum power point tracking (MPPT) is one of the key functions of the solar power management system in solar energy deployment. This paper investigates the design of fuzzy-logic-based solar power MPPT algorithms using different fuzzy input variables. Six fuzzy MPPT algorithms, based on different input variables, were considered in this study, namely (i) slope (of solar power-versus-solar voltage) and changes of the slope; (ii) slope and variation of the power; (iii) variation of power and variation of voltage; (iv) variation of power and variation of current; (v) sum of conductance and increment of the conductance; and (vi) sum of angles of arctangent of the conductance and arctangent of increment of the conductance. Algorithms (i)–(iv) have two input variables each while algorithms (v) and (vi) use a single input variable. The fuzzy logic MPPT function is deployed using a buck-boost power converter. This paper presents the details of the determinations, considerations of the fuzzy rules, as well as advantages and disadvantages of each MPPT algorithm based upon photovoltaic (PV) cell properties. The range of the input variable of Algorithm (vi) is finite and the maximum power point condition is well defined in steady condition and, therefore, it can be used for multipurpose controller design. Computer simulations are conducted to verify the design.
Convergence Aspects for Generalizations of q-Hypergeometric Functions
Authors: Ernst Thomas
Published: April 2015

In an earlier paper, we found transformation and summation formulas for 43 q-hypergeometric functions of 2n variables. The aim of the present article is to find convergence regions and a few conjectures of convergence regions for these functions based on a vector version of the Nova q-addition. These convergence regions are given in a purely formal way, extending the results of Karlsson (1976). The Γq-function and the q-binomial coefficients, which are used in the proofs, are adjusted accordingly. Furthermore, limits and special cases for the new functions, e.g., q-Lauricella functions and q-Horn functions, are pointed out.
Neuroendocrine Carcinomas of the Gastroenteropancreatic System: A Comprehensive Review
Authors: Ilett Emma , Langer Seppo , Olsen Ingrid , Federspiel Birgitte , Kjær Andreas , Knigge Ulrich
Published: April 2015

To date, empirical literature has generally been considered lacking in relation to neuroendocrine carcinomas (NECs), the highly malignant subgroup of neuroendocrine neoplasms. NECs are often found in the lungs or the gastroenteropancreatic (GEP) system and can be of small or large cell type. Concentrating on GEP-NECs, we can conclude that survival times are poor, with a median of only 4–16 months depending on disease stage and primary site. Further, this aggressive disease appears to be on the rise, with incidence numbers increasing while survival times are stagnant. Treatment strategies concerning surgery are often undecided and second-line chemotherapy is not yet established. After an analysis of over 2600 articles, we can conclude that there is indeed more empirical literature concerning GEP-NECs available than previously assumed. This unique review is based on 333 selected articles and contains detailed information concerning all aspects of GEP-NECs. Namely, the classification, histology, genetic abnormalities, epidemiology, origin, biochemistry, imaging, treatment and survival of GEP-NECs are described. Also, organ-specific summaries with more detail in relation to disease presentation, diagnosis, treatment and survival are presented. Finally, key points are discussed with directions for future research priorities.
Melting Behavior and Thermolysis of NaBH4−Mg(BH4)2 and NaBH4−Ca(BH4)2 Composites
Authors: Ley Morten , Roedern Elsa , Thygesen Peter , Jensen Torben
Published: April 2015

The physical properties and the hydrogen release of NaBH4–Mg(BH4)2 and NaBH4−Ca(BH4)2 composites are investigated using in situ synchrotron radiation powder X-ray diffraction, thermal analysis and temperature programmed photographic analysis. The composite, xNaBH4–(1 − x)Mg(BH4)2, x = 0.4 to 0.5, shows melting/frothing between 205 and 220 °C. However, the sample does not become a transparent molten phase. This behavior is similar to other alkali-alkaline earth metal borohydride composites. In the xNaBH4–(1 − x)Ca(BH4)2 system, eutectic melting is not observed. Interestingly, eutectic melting in metal borohydrides systems leads to partial thermolysis and hydrogen release at lower temperatures and the control of sample melting may open new routes for obtaining high-capacity hydrogen storage materials.
Evaluating the Ecological Integrity of Structural Stand Density Management Models Developed for Boreal Conifers
Authors: Newton Peter
Published: April 2015

Density management decision-support systems (e.g., modular-based structural stand density management models (SSDMMs)), which are built upon the modeling platform used to develop stand density management diagrams, incorporate a number of functional relationships derived from forest production theory and quantitative ecology. Empirically, however, the ecological integrity of these systems has not been verified and hence the degree of their compliance with expected ecological axioms is unknown. Consequently, the objective of this study was to evaluate the ecological integrity of six SSDMMs developed for black spruce (Picea mariana) and jack pine (Pinus banksiana) stand-types (natural-origin and planted upland black spruce and jack pine stands, upland natural-origin black spruce and jack pine mixtures, and natural-origin lowland black spruce stands). The assessment included the determination of the biological reasonableness of model predictions by determining the degree of consistency between predicted developmental patterns and those expected from known ecological axioms derived from even-aged stand dynamics theoretical constructs, employing Bakuzis graphical matrices. Although the results indicated the SSDMMs performed well, a notable departure from expectation was a possible systematic site quality effect on the asymptotic yield-density relationships. Combining these results with confirmatory evidence derived from the literature suggest that the site-invariant self-thinning axiom may be untenable for certain stand-types.
Taking Stock of Carbon Rights in REDD+ Candidate Countries: Concept Meets Reality
Authors: Loft Lasse , Ravikumar Ashwin , Gebara Maria , Pham Thu , Resosudarmo Ida , Assembe Samuel , Tovar Jazmín , Mwangi Esther , Andersson Krister
Published: April 2015

In the discourses on who should benefit from national REDD+ implementation, rights-based approaches are prominent across various countries. Options on how to create viable property rights arrangements are currently being debated by scholars, policy makers and practitioners alike. Many REDD+ advocates argue that assigning carbon rights represents a solution to insecure individual and community property rights. But carbon rights, i.e., the bundle of legal rights to carbon sequestered in biomass, present their own set of theoretical and practical challenges. We assess the status and approaches chosen in emerging carbon-rights legislations in five REDD+ countries based on a literature review and country expert knowledge: Peru, Brazil, Cameroon, Vietnam and Indonesia. We find that most countries assessed have not yet made final decisions as to the type of benefit sharing mechanisms they intend to implement and that there is a lack of clarity about who owns rights to carbon as a property and who is entitled to receive benefits. However, there is a trend of linking carbon rights to land rights. As such, the technical and also political challenges that land tenure clarification has faced over the past decades will still need to be addressed in the context of carbon rights.
Effect of a Multi-Level Education Intervention Model on Knowledge and Attitudes of Accidental Injuries in Rural Children in Zunyi, Southwest China
Authors: Cao Bo-Ling , Shi Xiu-Quan , Qi Yong-Hong , Hui Ya , Yang Hua-Jun , Shi Shang-Peng , Luo Li-Rong , Zhang Hong , Wang Xin , Yang Ying-Ping
Published: April 2015

Objective: To explore the effect of a school-family-individual (SFI) multi-level education intervention model on knowledge and attitudes about accidental injuries among school-aged children to improve injury prevention strategies and reduce the incidence of pediatric injuries. Methods: The random sample of rural school-aged children were recruited by using a multistage, stratified, cluster sampling method in Zunyi, Southwest China from 2012 to 2014, and 2342 children were randomly divided into intervention and control groups. Then children answered a baseline survey to collect knowledge and attitude scores (KAS) of accidental injuries. In the intervention group, children, their parents/guardians and the school received a SFI multi-level education intervention, which included a children’s injury-prevention poster at schools, an open letter about security instruction for parents/guardians and multiple-media health education (Microsoft PowerPoint lectures, videos, handbooks, etc.) to children. Children in the control group were given only handbook education. After 16 months, children answered a follow-up survey to collect data on accidental injury types and accidental injury-related KAS for comparing the intervention and control groups and baseline and follow-up data. Results: The distribution of gender was not significantly different while age was different between the baseline and follow-up survey. At baseline, the mean KAS was lower for the intervention than control group (15.37 ± 3.40 and 18.35 ± 5.01; p < 0.001). At follow-up, the mean KAS was higher for the intervention than control group (21.16 ± 3.05 and 20.02 ± 3.40; p < 0.001). The increase in KAS in the intervention and control groups was significant (p < 0.001; KAS: 5.79 vs. 1.67) and suggested that children’s injury-related KAS improved in the intervention group. Moreover, the KAS between the groups differed for most subtypes of incidental injuries (based on International Classification of Diseases 10, ICD-10) (p < 0.05). Before intervention, 350 children had reported their accident injury episodes, while after intervention 237 children had reported their accidental injury episodes in the follow-up survey. Conclusions: SFI multi-level education intervention could significantly increase KAS for accidental injuries, which should improve children’s prevention-related knowledge and attitudes about such injuries. It should help children change their risk behaviors and reduce the incidence of accidental injuries. Our results highlight a new intervention model of injury prevention among school-aged children.
Interactive Presentation of Geo-Spatial Climate Data in Multi-Display Environments
Authors: Eichner Christian , Nocke Thomas , Schulz Hans-Jörg , Schumann Heidrun
Published: April 2015

The visual analysis of complex geo-spatial data is a challenging task. Typically, different views are used to communicate different aspects. With changing topics of interest, however, novel views are required. This leads to dynamically changing presentations of multiple views. This paper introduces a novel approach to support such scenarios. It allows for a spontaneous incorporation of views from different sources and to automatically layout these views in a multi-display environment. Furthermore, we introduce an enhanced undo/redo mechanism for this setting, which records user interactions and, in this way, enables swift reconfigurations of displayed views. Hence, users can fluently switch the focus of visual analysis without extensive manual interactions. We demonstrate our approach by the particular use case of discussing geo-spatial climate data.
The WRKY Transcription Factor Genes in Eggplant (Solanum melongena L.) and Turkey Berry (Solanum torvum Sw.)
Authors: Yang Xu , Deng Cao , Zhang Yu , Cheng Yufu , Huo Qiuyue , Xue Linbao
Published: April 2015

WRKY transcription factors, which play critical roles in stress responses, have not been characterized in eggplant or its wild relative, turkey berry. The recent availability of RNA-sequencing data provides the opportunity to examine WRKY genes from a global perspective. We identified 50 and 62 WRKY genes in eggplant (SmelWRKYs) and turkey berry (StorWRKYs), respectively, all of which could be classified into three groups (I–III) based on the WRKY protein structure. The SmelWRKYs and StorWRKYs contain ~76% and ~95% of the number of WRKYs found in other sequenced asterid species, respectively. Positive selection analysis revealed that different selection constraints could have affected the evolution of these groups. Positively-selected sites were found in Groups IIc and III. Branch-specific selection pressure analysis indicated that most WRKY domains from SmelWRKYs and StorWRKYs are conserved and have evolved at low rates since their divergence. Comparison to homologous WRKY genes in Arabidopsis revealed several potential pathogen resistance-related SmelWRKYs and StorWRKYs, providing possible candidate genetic resources for improving stress tolerance in eggplant and probably other Solanaceae plants. To our knowledge, this is the first report of a genome-wide analyses of the SmelWRKYs and StorWRKYs.
Phenotypic and Molecular Convergence of 2q23.1 Deletion Syndrome with Other Neurodevelopmental Syndromes Associated with Autism Spectrum Disorder
Authors: Mullegama Sureni , Alaimo Joseph , Chen Li , Elsea Sarah
Published: April 2015

Roughly 20% of autism spectrum disorders (ASD) are syndromic with a well-established genetic cause. Studying the genes involved can provide insight into the molecular and cellular mechanisms of ASD. 2q23.1 deletion syndrome (causative gene, MBD5) is a recently identified genetic neurodevelopmental disorder associated with ASD. Mutations in MBD5 have been found in ASD cohorts. In this study, we provide a phenotypic update on the prevalent features of 2q23.1 deletion syndrome, which include severe intellectual disability, seizures, significant speech impairment, sleep disturbance, and autistic-like behavioral problems. Next, we examined the phenotypic, molecular, and network/pathway relationships between nine neurodevelopmental disorders associated with ASD: 2q23.1 deletion Rett, Angelman, Pitt-Hopkins, 2q23.1 duplication, 5q14.3 deletion, Kleefstra, Kabuki make-up, and Smith-Magenis syndromes. We show phenotypic overlaps consisting of intellectual disability, speech delay, seizures, sleep disturbance, hypotonia, and autistic-like behaviors. Molecularly, MBD5 possibly regulates the expression of UBE3A, TCF4, MEF2C, EHMT1 and RAI1. Network analysis reveals that there could be indirect protein interactions, further implicating function for these genes in common pathways. Further, we show that when MBD5 and RAI1 are haploinsufficient, they perturb several common pathways that are linked to neuronal and behavioral development. These findings support further investigations into the molecular and pathway relationships among genes linked to neurodevelopmental disorders and ASD, which will hopefully lead to common points of regulation that may be targeted toward therapeutic intervention.
Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced Dermal Elastase Activity, Triggers Wrinkling and Sagging
Authors: Imokawa Genji , Ishida Koichi
Published: April 2015

The repetitive exposure of skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat skin there is a similar tortuous deformation of elastic fibers together with decreased skin elasticity, whose magnitudes are greater in UVB-exposed skin than in UVA-exposed skin. Comparison of skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal skin and human facial skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of skin fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of skin fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the skin via the impairment of elastic fiber configuration and the subsequent loss of skin elasticity.
Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging II: Over-Expression of Neprilysin Plays an Essential Role
Authors: Imokawa Genji , Nakajima Hiroaki , Ishida Koichi
Published: April 2015

Our previous studies strongly indicated that the up-regulated activity of skin fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the skin via the impairment of elastic fiber configuration and the subsequent loss of skin elasticity. Fortunately, we succeeded in identifying human skin fibroblast-derived elastase as a previously known enzyme, neprilysin or neutral endopeptidase (NEP). We have also characterized epithelial-mesenchymal paracrine cytokine interactions between UVB-exposed-keratinocytes and dermal fibroblasts and found that interleukin-1α and granulocyte macrophage colony stimulatory factor (GM-CSF) are intrinsic cytokines secreted by UVB-exposed keratinocytes that stimulate the expression of neprilysin by fibroblasts. On the other hand, direct UVA exposure of human fibroblasts significantly stimulates the secretion of IL-6 and also elicits a significant increase in the gene expression of matrix metallo-protease(MMP)-1 as well as neprilysin (to a lesser extent), which is followed by distinct increases in their protein and enzymatic activity levels. Direct UVA exposure of human keratinocytes also stimulates the secretion of IL-6, IL-8 and GM-CSF but not of IL-1 and endothelin-1. These findings suggest that GM-CSF secreted by UVA-exposed keratinocytes as well as IL-6 secreted by UVA-exposed dermal fibroblasts play important and additional roles in UVA-induced sagging and wrinkling by up-regulation of neprilysin and MMP-1, respectively, in dermal fibroblasts.
Carbohydrate-Derived Amphiphilic Macromolecules: A Biophysical Structural Characterization and Analysis of Binding Behaviors to Model Membranes
Authors: Martin Adriana , Tomasini Michael , Kholodovych Vladyslav , Gu Li , Sommerfeld Sven , Uhrich Kathryn , Murthy N. , Welsh William , Moghe Prabhas
Published: April 2015

The design and synthesis of enhanced membrane-intercalating biomaterials for drug delivery or vascular membrane targeting is currently challenged by the lack of screening and prediction tools. The present work demonstrates the generation of a Quantitative Structural Activity Relationship model (QSAR) to make a priori predictions. Amphiphilic macromolecules (AMs) “stealth lipids” built on aldaric and uronic acids frameworks attached to poly(ethylene glycol) (PEG) polymer tails were developed to form self-assembling micelles. In the present study, a defined set of novel AM structures were investigated in terms of their binding to lipid membrane bilayers using Quartz Crystal Microbalance with Dissipation (QCM-D) experiments coupled with computational coarse-grained molecular dynamics (CG MD) and all-atom MD (AA MD) simulations. The CG MD simulations capture the insertion dynamics of the AM lipophilic backbones into the lipid bilayer with the PEGylated tail directed into bulk water. QCM-D measurements with Voigt viscoelastic model analysis enabled the quantitation of the mass gain and rate of interaction between the AM and the lipid bilayer surface. Thus, this study yielded insights about variations in the functional activity of AM materials with minute compositional or stereochemical differences based on membrane binding, which has translational potential for transplanting these materials in vivo. More broadly, it demonstrates an integrated computational-experimental approach, which can offer a promising strategy for the in silico design and screening of therapeutic candidate materials.
3D Printed Unibody Lab-on-a-Chip: Features Survey and Check-Valves Integration
Authors: Comina Germán , Suska Anke , Filippini Daniel
Published: April 2015

The unibody lab-on-a-chip (ULOC) concept entails a fast and affordable micro-prototyping system built around a single monolithic 3D printed element (unibody). A consumer-grade stereo lithography (SL) 3D printer can configure ULOCs with different forms of sample delivery, transport, handling and readout, while minimizing material costs and fabrication time. ULOC centralizes all complex fabrication procedures and replaces the need for clean room resources, delivering prototypes for less than 1 US$, which can be printed in 10 min and ready for testing in less than 30 min. Recent examples of ULOC integration of transport, chemical sensing for optical readout and flow mixing capabilities are discussed, as well as the integration of the first check-valves for ULOC devices. ULOC valves are strictly unidirectional up to 100 psi, show an exponential forward flow behavior up to 70 psi and can be entirely fabricated with the ULOC approach.
Site-Specific Bioconjugation of an Organometallic Electron Mediator to an Enzyme with Retained Photocatalytic Cofactor Regenerating Capacity and Enzymatic Activity
Authors: Lim Sung , Yoon Sungho , Kim Yong , Kwon Inchan
Published: April 2015

Photosynthesis consists of a series of reactions catalyzed by redox enzymes to synthesize carbohydrates using solar energy. In order to take the advantage of solar energy, many researchers have investigated artificial photosynthesis systems mimicking the natural photosynthetic enzymatic redox reactions. These redox reactions usually require cofactors, which due to their high cost become a key issue when constructing an artificial photosynthesis system. Combining a photosensitizer and an Rh-based electron mediator (RhM) has been shown to photocatalytically regenerate cofactors. However, maintaining the high concentration of cofactors available for efficient enzymatic reactions requires a high concentration of the expensive RhM; making this process cost prohibitive. We hypothesized that conjugation of an electron mediator to a redox enzyme will reduce the amount of electron mediators necessary for efficient enzymatic reactions. This is due to photocatalytically regenerated NAD(P)H being readily available to a redox enzyme, when the local NAD(P)H concentration near the enzyme becomes higher. However, conventional random conjugation of RhM to a redox enzyme will likely lead to a substantial loss of cofactor regenerating capacity and enzymatic activity. In order to avoid this issue, we investigated whether bioconjugation of RhM to a permissive site of a redox enzyme retains cofactor regenerating capacity and enzymatic activity. As a model system, a RhM was conjugated to a redox enzyme, formate dehydrogenase obtained from Thiobacillus sp. KNK65MA (TsFDH). A RhM-containing azide group was site-specifically conjugated to p-azidophenylalanine introduced to a permissive site of TsFDH via a bioorthogonal strain-promoted azide-alkyne cycloaddition and an appropriate linker. The TsFDH-RhM conjugate exhibited retained cofactor regenerating capacity and enzymatic activity.
Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions
Authors: Zhang Junchao , Xie Wengang , Wang Yanrong , Zhao Xuhong
Published: April 2015

Elymus sibiricus as an important forage grass and gene pool for improving cereal crops, that is widely distributed in West and North China. Information on its genetic diversity and relationships is limited but necessary for germplasm collection, conservation and future breeding. Start Codon Targeted (SCoT) markers were used for studying the genetic diversity and relationships among 53 E. sibiricus accessions from its primary distribution area in China. A total of 173 bands were generated from 16 SCoT primers, 159 bands of which were polymorphic with the percentage of polymorphic bands (PPB) of 91.91%. Based upon population structure analysis five groups were formed. The cluster analysis separated the accessions into two major clusters and three sub-clusters, similar to results of principal coordinate analysis (PCoA). The molecular variance analysis (AMOVA) showed that genetic variation was greater within geographical regions (50.99%) than between them (49.01%). Furthermore, the study also suggested that collecting and evaluating E. sibiricus germplasm for major geographic regions and special environments broadens the available genetic base and illustrates the range of variation. The results of the present study showed that SCoT markers were efficient in assessing the genetic diversity among E. sibiricus accessions.
Nanocapsular Dispersion of Cinnamaldehyde for Enhanced Inhibitory Activity against Aflatoxin Production by Aspergillus flavus
Authors: Li Hongbo , Shen Qingshan , Zhou Wei , Mo Haizhen , Pan Daodong , Hu Liangbin
Published: April 2015

Cinnamaldehyde (CA) is marginally soluble in water, making it challenging to evenly disperse it in foods, and resulting in lowered anti-A. flavus efficacy. In the present study, nano-dispersed CA (nano-CA) was prepared to increase its aqueous solubility. Free and nano-dispersed CA were compared in terms of their inhibitory activity against fungal growth and aflatoxin production of A. flavus both in Sabouraud Dextrose (SD) culture and in peanut butter. Our results indicated that free CA inhibited the mycelia growth and aflatoxin production of A. flavus with a minimal inhibitory concentration (MIC) value of 1.0 mM, but promoted the aflatoxin production at some concentrations lower than the MIC. Nano-CA had a lower MIC value of 0.8 mM against A. flavus, and also showed improved activity against aflatoxin production without the promotion at lower dose. The solidity of peanut butter had an adverse impact on the antifungal activity of free CA, whereas nano-dispersed CA showed more than 2-fold improved activity against the growth of A. flavus. Free CA still promoted AFB1 production at the concentration of 0.25 mM, whereas nano-CA showed more efficient inhibition of AFB1 production in the butter.
Development and Characterization of Polymorphic Genic-SSR Markers in Larix kaempferi
Authors: Chen Xing-Bin , Xie Yun-Hui , Sun Xiao-Mei
Published: April 2015

New simple sequence repeat (SSR) markers were developed in the Japanese larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five (1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 (90.6%) exhibited polymorphism among 24 individuals of L. kaempferi, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) averaging at 4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of cross-species amplification were observed. The genic-SSRs developed herein would be a valuable resource for genetic analysis of Larix kaempferi and related species, and also have the potential to facilitate the genetic improvement and breeding of larch.
Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L.
Authors: Soural Ivo , Vrchotová Naděžda , Tříska Jan , Balík Josef , Horník Štěpán , Cuřínová Petra , Sýkora Jan
Published: April 2015

Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.
Bactericidal Effect of Extracts and Metabolites of Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory Diseases
Authors: Patra Jayanta , Kim Eun , Oh Kyounghee , Kim Hyeon-Jeong , Dhakal Radhika , Kim Yangseon , Baek Kwang-Hyun
Published: April 2015

The mouth cavity hosts many types of anaerobic bacteria, including Streptococcus mutans and Porphyromonas gingivalis, which cause periodontal inflammatory diseases and dental caries. The present study was conducted to evaluate the antibacterial potential of extracts of Robinia pseudoacacia and its different fractions, as well as some of its natural compounds against oral pathogens and a nonpathogenic reference bacteria, Escherichia coli. The antibacterial activity of the crude extract and the solvent fractions (hexane, chloroform, ethyl acetate and butanol) of R. pseudoacacia were evaluated against S. mutans, P. gingivalis and E. coli DH5α by standard micro-assay procedure using conventional sterile polystyrene microplates. The results showed that the crude extract was more active against P. gingivalis (100% growth inhibition) than against S. mutans (73% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fractions were active against P. gingivalis, with 91 and 97% growth inhibition, respectively, at 0.2 mg/mL. None of seven natural compounds found in R. pseudoacacia exerted an antibacterial effect on P. gingivalis; however, fisetin and myricetin at 8 µg/mL inhibited the growth of S. mutans by 81% and 86%, respectively. The crude extract of R. pseudoacacia possesses bioactive compounds that could completely control the growth of P. gingivalis. The antibiotic activities of the hexane and chloroform fractions suggest that the active compounds are hydrophobic in nature. The results indicate the effectiveness of the plant in clinical applications for the treatment of dental plaque and periodontal inflammatory diseases and its potential use as disinfectant for various surgical and orthodontic appliances.
Synthesis of Novel Perfluoroalkylglucosides on Zeolite and Non-Zeolite Catalysts
Authors: Nowicki Janusz , Mokrzycki Łukasz , Sulikowski Bogdan
Published: April 2015

Perfluoroalkylglucosides comprise a very important class of fluorine-containing surfactants. These compounds can be synthesized by using the Fisher reaction, starting directly from glucose and the required perfluoroalcohols. We wish to report on the use of zeolite catalysts of different structure and composition for the synthesis of perfluoroalkylglucosides when using glucose and 1-octafluoropentanol as substrates. Zeolites of different pore architecture have been chosen (ZSM-5, ZSM-12, MCM-22 and Beta). Zeolites were characterized by XRD, nitrogen sorption, scanning electron microscopy (SEM) and solid-state 27Al MAS NMR spectroscopy. The activity of the zeolite catalysts in the glycosidation reaction was studied in a batch reactor at 100 °C below atmospheric pressure. The performance of zeolites was compared to other catalysts, an ion-exchange resin (Purolite) and a montmorillonite-type layered aluminosilicate. The catalytic performance of zeolite Beta was the highest among the zeolites studied and the results were comparable to those obtained over Purolite and montmorillonite type catalysts.
Increased Intake of Selected Vegetables, Herbs and Fruit may Reduce Bone Turnover in Post-Menopausal Women
Authors: Gunn Caroline , Weber Janet , McGill Anne-Thea , Kruger Marlena
Published: April 2015

Increased consumption of vegetables/herbs/fruit may reduce bone turnover and urinary calcium loss in post-menopausal women because of increased intake of polyphenols and potassium, but comparative human studies are lacking. The main aim was to compare bone turnover markers and urinary calcium excretion in two randomised groups (n = 50) of healthy post-menopausal women consuming ≥9 servings of different vegetables/herbs/fruit combinations (three months). Group A emphasised a generic range of vegetables/herbs/fruit, whereas Group B emphasised specific vegetables/herbs/fruit with bone resorption-inhibiting properties (Scarborough Fair Diet), with both diets controlled for potential renal acid load (PRAL). Group C consumed their usual diet. Plasma bone markers, urinary electrolytes (24 h) and estimated dietary PRAL were assessed at baseline and 12 weeks. Procollagen type I N propeptide (PINP) decreased (−3.2 μg/L, p < 0.01) in the B group only, as did C-terminal telopeptide of type I collagen (CTX) (−0.065 μg/L, p < 0.01) in women with osteopenia compared to those with normal bone mineral density (BMD) within this group. Intervention Groups A and B had decreased PRAL, increased urine pH and significantly decreased urinary calcium loss. Urinary potassium increased in all groups, reflecting a dietary change. In conclusion, Group B demonstrated positive changes in both turnover markers and calcium conservation.
Image-Based Navigation for the SnowEater Robot Using a Low-Resolution USB Camera
Authors: Rivas Ernesto , Komagome Koutarou , Mitobe Kazuhisa , Capi Genci
Published: April 2015

This paper reports on a navigation method for the snow-removal robot called SnowEater. The robot is designed to work autonomously within small areas (around 30 m2 or less) following line segment paths. The line segment paths are laid out so as much snow as possible can be cleared from an area. Navigation is accomplished by using an onboard low-resolution USB camera and a small marker located in the area to be cleared. Low-resolution cameras allow only limited localization and present significant errors. However, these errors can be overcome by using an efficient navigation algorithm to exploit the merits of these cameras. For stable robust autonomous snow removal using this limited information, the most reliable data are selected and the travel paths are controlled. The navigation paths are a set of radially arranged line segments emanating from a marker placed in the environment area to be cleared, in a place where it is not covered by snow. With this method, by using a low-resolution camera (640 × 480 pixels) and a small marker (100 × 100 mm), the robot covered the testing area following line segments. For a reference angle of 4.5° between line paths, the average results are: 4° for motion on hard floor and 4.8° for motion on compacted snow. The main contribution of this study is the design of a path-following control algorithm capable of absorbing the errors generated by a low-cost camera.
Nonlinear Optimization-Based Device-Free Localization with Outlier Link Rejection
Authors: Xiao Wendong , Song Biao , Yu Xiting , Chen Peiyuan
Published: April 2015

Device-free localization (DFL) is an emerging wireless technique for estimating the location of target that does not have any attached electronic device. It has found extensive use in Smart City applications such as healthcare at home and hospitals, location-based services at smart spaces, city emergency response and infrastructure security. In DFL, wireless devices are used as sensors that can sense the target by transmitting and receiving wireless signals collaboratively. Many DFL systems are implemented based on received signal strength (RSS) measurements and the location of the target is estimated by detecting the changes of the RSS measurements of the wireless links. Due to the uncertainty of the wireless channel, certain links may be seriously polluted and result in erroneous detection. In this paper, we propose a novel nonlinear optimization approach with outlier link rejection (NOOLR) for RSS-based DFL. It consists of three key strategies, including: (1) affected link identification by differential RSS detection; (2) outlier link rejection via geometrical positional relationship among links; (3) target location estimation by formulating and solving a nonlinear optimization problem. Experimental results demonstrate that NOOLR is robust to the fluctuation of the wireless signals with superior localization accuracy compared with the existing Radio Tomographic Imaging (RTI) approach.
Sensing Properties of Pd-Loaded Co3O4 Film for a ppb-Level NO Gas Sensor
Authors: Akamatsu Takafumi , Itoh Toshio , Izu Noriya , Shin Woosuck , Sato Kazuo
Published: April 2015

We prepared 0.1 wt%–30 wt% Pd-loaded Co3O4 by a colloidal mixing method and investigated the sensing properties of a Pd-loaded Co3O4 sensor element, such as the sensor response, 90% response time, 90% recovery time, and signal-to-noise (S/N) ratio, toward low nitric oxide (NO) gas levels in the range from 50 to 200 parts per billion. The structural properties of the Pd-loaded Co3O4 powder were investigated using X-ray diffraction analysis and transmission electron microscopy. Pd in the powder existed as PdO. The sensor elements with 0.1 wt%–10 wt% Pd content have higher sensor properties than those without any Pd content. The response of the sensor element with a 30 wt% Pd content decreased markedly because of the aggregation and poor dispersibility of the PdO particles. High sensor response and S/N ratio toward the NO gas were achieved when a sensor element with 10 wt% Pd content was used.
A Kinect-Based Real-Time Compressive Tracking Prototype System for Amphibious Spherical Robots
Authors: Pan Shaowu , Shi Liwei , Guo Shuxiang
Published: April 2015

A visual tracking system is essential as a basis for visual servoing, autonomous navigation, path planning, robot-human interaction and other robotic functions. To execute various tasks in diverse and ever-changing environments, a mobile robot requires high levels of robustness, precision, environmental adaptability and real-time performance of the visual tracking system. In keeping with the application characteristics of our amphibious spherical robot, which was proposed for flexible and economical underwater exploration in 2012, an improved RGB-D visual tracking algorithm is proposed and implemented. Given the limited power source and computational capabilities of mobile robots, compressive tracking (CT), which is the effective and efficient algorithm that was proposed in 2012, was selected as the basis of the proposed algorithm to process colour images. A Kalman filter with a second-order motion model was implemented to predict the state of the target and select candidate patches or samples for the CT tracker. In addition, a variance ratio features shift (VR-V) tracker with a Kalman estimation mechanism was used to process depth images. Using a feedback strategy, the depth tracking results were used to assist the CT tracker in updating classifier parameters at an adaptive rate. In this way, most of the deficiencies of CT, including drift and poor robustness to occlusion and high-speed target motion, were partly solved. To evaluate the proposed algorithm, a Microsoft Kinect sensor, which combines colour and infrared depth cameras, was adopted for use in a prototype of the robotic tracking system. The experimental results with various image sequences demonstrated the effectiveness, robustness and real-time performance of the tracking system.
Cross-Layer Cluster-Based Energy-Efficient Protocol for Wireless Sensor Networks
Authors: Mammu Aboobeker , Hernandez-Jayo Unai , Sainz Nekane , De la Iglesia Idoia
Published: April 2015

Recent developments in electronics and wireless communications have enabled the improvement of low-power and low-cost wireless sensors networks (WSNs). One of the most important challenges in WSNs is to increase the network lifetime due to the limited energy capacity of the network nodes. Another major challenge in WSNs is the hot spots that emerge as locations under heavy traffic load. Nodes in such areas quickly drain energy resources, leading to disconnection in network services. In such an environment, cross-layer cluster-based energy-efficient algorithms (CCBE) can prolong the network lifetime and energy efficiency. CCBE is based on clustering the nodes to different hexagonal structures. A hexagonal cluster consists of cluster members (CMs) and a cluster head (CH). The CHs are selected from the CMs based on nodes near the optimal CH distance and the residual energy of the nodes. Additionally, the optimal CH distance that links to optimal energy consumption is derived. To balance the energy consumption and the traffic load in the network, the CHs are rotated among all CMs. In WSNs, energy is mostly consumed during transmission and reception. Transmission collisions can further decrease the energy efficiency. These collisions can be avoided by using a contention-free protocol during the transmission period. Additionally, the CH allocates slots to the CMs based on their residual energy to increase sleep time. Furthermore, the energy consumption of CH can be further reduced by data aggregation. In this paper, we propose a data aggregation level based on the residual energy of CH and a cost-aware decision scheme for the fusion of data. Performance results show that the CCBE scheme performs better in terms of network lifetime, energy consumption and throughput compared to low-energy adaptive clustering hierarchy (LEACH) and hybrid energy-efficient distributed clustering (HEED).
The Effects of Air Permeability, Background Ventilation and Lifestyle on Energy Performance, Indoor Air Quality and Risk of Condensation in Domestic Buildings
Authors: Hashemi Arman , Khatami Narguess
Published: April 2015

Effective and efficient ventilation is essential when improving energy performance and Indoor Air Quality (IAQ) of buildings. Reducing air permeability can considerably improve the energy performance of buildings; however, making the buildings more airtight may result in lower rates of natural ventilation which may in turn increase the risks of condensation and unacceptable IAQ. This study evaluates the effects of different air permeability rates, background ventilation and occupants’ lifestyles on the energy performance as well as the risk of condensation and CO2 concentration in domestic buildings. Dynamic computer simulations were conducted in EnergyPlus. Results indicated direct relations between the ventilation rates, energy performance and IAQ. Higher air permeability along with background ventilation resulted in considerably better IAQ while energy consumption increased by up to four times. Occupants’ lifestyles were identified as a major contributor to the risk of condensation.
FEM Analysis of Fluid-Structure Interaction in Thermal Heavy Oil Recovery Operations
Authors: Yin Yao , Liu Yiliang
Published: April 2015

In the process of heavy oil thermal recovery, the creep of strata can often be accelerated due to injection pressure, the temperature of steam, the structural characteristics of rock itself and other factors. However, the effect of creep in strata may cause various types of damage in underground mining, such as fracture or deformation of oil casings, and so on. The mechanism of fluid–structure interaction provides a powerful theoretical guidance for stratum creep, which happens during the process of heavy oil thermal recovery. According to existing research, a practical engineering finite element model of Jin.25 Block in Liaohe Oilfield was built based on the finite element analysis software ADINA, and the numerical simulation of the thermal recovery stratum creep of Jin.25 Block was done using the fluid–structure interaction (FSI) calculation module. The results were compared and analyzed, combining each influencing factor of the stratum creep in practical engineering. It is proposed that steam injection pressure should not exceed 14 MPa while enhancing oil recovery by increasing the injection pressure; the main reason is that temperature impact on casings is closely related to the change in stratum creep stress. However, particular attention should be paid to the thermal sensitivity of casing itself and creep deformation when the hydraulic conductivity magnitude is above 1 × 10−9 m/s, so as to effectively prevent or minimize the economic loss caused by stratum creep.