bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunopathological Alterations after Blast Injury and Hemorrhage in a Swine Model of Prolonged Damage Control Resuscitation
Authors: Simovic Milomir O. , Yang Zhangsheng , Jordan Bryan S. , Fraker Tamara L. , Cancio Tomas S. , Lucas Michael L. , Cancio Leopoldo C. , Li Yansong
Published: April 2023

Trauma-related hemorrhagic shock (HS) remains a leading cause of death among military and civilian trauma patients. We have previously shown that administration of complement and HMGB1 inhibitors attenuate morbidity and mortality 24 h after injury in a rat model of blast injury (BI) and HS. To further validate these results, this study aimed to develop a swine model and evaluate BI+HS-induced pathophysiology. Anesthetized Yucatan minipigs underwent combined BI and volume-controlled hemorrhage. After 30 min of shock, animals received an intravenous bolus of PlasmaLyte A and a continuous PlasmaLyte A infusion. The survival rate was 80% (4/5), and the non-survivor expired 72 min post-BI. Circulating organ-functional biomarkers, inflammatory biomarkers, histopathological evaluation, and CT scans indicated evidence of multiple-organ damage, systemic innate immunological activation, and local tissue inflammation in the injured animals. Interestingly, a rapid and dramatic increase in plasma levels of HMGB1 and C3a and markedly early myocarditis and encephalitis were associated with early death post-BI+HS. This study suggests that this model reflects the immunopathological alterations of polytrauma in humans during shock and prolonged damage control resuscitation. This experimental protocol could be helpful in the assessment of immunological damage control resuscitation approaches during the prolonged care of warfighters.
Isostructural Oxides Sr3Ti2−xMxO7−δ (M = Mn, Fe, Co; x = 0, 1) as Electrocatalysts for Water Splitting
Authors: Kananke-Gamage Chandana C. W. , Ramezanipour Farshid
Published: April 2023

The correlation of the electrocatalytic activity with electrical conductivity, oxygen-vacancies, and electronegativity have been studied in a series of isostructural oxides, having the so-called Ruddlesden-Popper structure. The structures of these materials comprise transition metals that are octahedrally coordinated to form a network of bilayer stacks. These materials are catalytically active for both half-reactions of water-splitting, namely oxygen-evolution reaction (OER) and hydrogen-evolution reaction (HER). They show a systematic increase in electrocatalytic activity in progression from Sr3Ti2O7 to Sr3TiMnO7, Sr3TiFeO7−δ, and Sr3TiCoO7−δ. The kinetic studies using the Tafel method indicate the same trend across the series, where the best catalyst also has the fastest kinetics for both HER and OER. In addition, the same progression is observed in the concentration of oxygen-vacancies, as well as the electrical conductivity in a wide range of temperatures, 25 °C–800 °C. The material that shows the best electrocatalytic activity, i.e., Sr3TiCoO7−δ, also has the highest electrical conductivity and the greatest concentration of oxygen vacancies in the series. The correlations observed in this work indicate that trends in electrocatalytic performance may be related to the systematic increase in electrical conductivity, electronegativity, and oxygen-vacancies, as well as the electron occupancy of eg orbitals, which can affect the strength of sigma interactions between the catalyst and reaction intermediates.
Association of Pre-Pregnancy Obesity and COVID-19 with Poor Pregnancy Outcome
Authors: Mihajlovic Sladjana , Nikolic Dejan , Milicic Biljana , Santric-Milicevic Milena , Glushkova Natalya , Nurgalieva Zhansaya , Lackovic Milan
Published: April 2023

Background and Objectives: During the COVID-19 pandemic, a possible overlap of obesity and COVID-19 infection has raised concerns among patients and healthcare professionals about protecting pregnant women from developing a severe infection and unwanted pregnancy outcomes. The aim of this study was to evaluate the associations of body mass index with clinical, laboratory, and radiology diagnostic parameters as well as pregnancy complications and maternal outcomes in pregnant patients with COVID-19. Materials and Methods: Clinical status, laboratory, and radiology diagnostic parameters and pregnancy outcomes were analyzed for pregnant women hospitalized between March 2020 and November 2021 in one tertiary-level university clinic in Belgrade, Serbia, due to infection with SARS-CoV-2. Pregnant women were divided into the three sub-groups according to their pre-pregnancy body mass index. For testing the differences between groups, a two-sided p-value <0.05 (the Kruskal–Wallis and ANOVA tests) was considered statistically significant. Results: Out of 192 hospitalized pregnant women, obese pregnant women had extended hospitalizations, including ICU duration, and they were more likely to develop multi-organ failure, pulmonary embolism, and drug-resistant nosocomial infection. Higher maternal mortality rates, as well as poor pregnancy outcomes, were also more likely to occur in the obese group of pregnant women. Overweight and obese pregnant women were more likely to develop gestational hypertension, and they had a higher grade of placental maturity. Conclusions: Obese pregnant women hospitalized due to COVID-19 infection were more likely to develop severe complications.
Paradigm Shifts in Cardiac Pacing: Where Have We Been and What Lies Ahead?
Authors: Ballantyne Brennan , Chew Derek , Vandenberk Bert
Published: April 2023

The history of cardiac pacing dates back to the 1930s with externalized pacing and has evolved to incorporate transvenous, multi-lead, or even leadless devices. Annual implantation rates of cardiac implantable electronic devices have increased since the introduction of the implantable system, likely related to expanding indications, and increasing global life expectancy and aging demographics. Here, we summarize the relevant literature on cardiac pacing to demonstrate the enormous impact it has had within the field of cardiology. Further, we look forward to the future of cardiac pacing, including conduction system pacing and leadless pacing strategies.
Machine Learning Identifies New Predictors on Restenosis Risk after Coronary Artery Stenting in 10,004 Patients with Surveillance Angiography
Authors: Güldener Ulrich , Kessler Thorsten , von Scheidt Moritz , Hawe Johann S. , Gerhard Beatrix , Maier Dieter , Lachmann Mark , Laugwitz Karl-Ludwig , Cassese Salvatore , Schömig Albert W. , Kastrati Adnan , Schunkert Heribert
Published: April 2023

Objective: Machine learning (ML) approaches have the potential to uncover regular patterns in multi-layered data. Here we applied self-organizing maps (SOMs) to detect such patterns with the aim to better predict in-stent restenosis (ISR) at surveillance angiography 6 to 8 months after percutaneous coronary intervention with stenting. Methods: In prospectively collected data from 10,004 patients receiving percutaneous coronary intervention (PCI) for 15,004 lesions, we applied SOMs to predict ISR angiographically 6–8 months after index procedure. SOM findings were compared with results of conventional uni- and multivariate analyses. The predictive value of both approaches was assessed after random splitting of patients into training and test sets (50:50). Results: Conventional multivariate analyses revealed 10, mostly known, predictors for restenosis after coronary stenting: balloon-to-vessel ratio, complex lesion morphology, diabetes mellitus, left main stenting, stent type (bare metal vs. first vs. second generation drug eluting stent), stent length, stenosis severity, vessel size reduction, and prior bypass surgery. The SOM approach identified all these and nine further predictors, including chronic vessel occlusion, lesion length, and prior PCI. Moreover, the SOM-based model performed well in predicting ISR (AUC under ROC: 0.728); however, there was no meaningful advantage in predicting ISR at surveillance angiography in comparison with the conventional multivariable model (0.726, p = 0.3). Conclusions: The agnostic SOM-based approach identified—without clinical knowledge—even more contributors to restenosis risk. In fact, SOMs applied to a large prospectively sampled cohort identified several novel predictors of restenosis after PCI. However, as compared with established covariates, ML technologies did not improve identification of patients at high risk for restenosis after PCI in a clinically relevant fashion.
Risky Decision-Making in Adults with Alcohol Use Disorder—A Systematic and Meta-Analytic Review
Authors: Ariesen Akke-Marij D. , Neubert Julia H. , Gaastra Geraldina F. , Tucha Oliver , Koerts Janneke
Published: April 2023

Alcohol use disorder (AUD) forms a major health concern and is the most common substance use disorder worldwide. The behavioural and cognitive deficits associated with AUD have often been related to impairments in risky decision-making. The aim of this study was to examine the magnitude and type of risky decision-making deficits of adults with AUD, as well as to explore the potential mechanisms behind these deficits. To this end, existing literature comparing risky decision-making task performance of an AUD group to a control group (CG) was systematically searched and analysed. A meta-analysis was performed to address overall effects. In total, 56 studies were included. In the majority of studies (i.e., 68%), the performance of the AUD group(s) deviated from the CG(s) on one or more of the adopted tasks, which was confirmed by a small to medium pooled effect size (Hedges’ g = 0.45). This review therefore provides evidence of increased risk taking in adults with AUD as compared to CGs. The increased risk taking may be due to deficits in affective and deliberative decision-making. Making use of ecologically valid tasks, future research should investigate whether risky decision-making deficits predate and/or are consequential to the addiction of adults with AUD.
Mild Water-Filtered Infrared-A Whole-Body Hyperthermia Reduces Pain in Patients with Fibromyalgia Syndrome—A Randomized Sham-Controlled Trial
Authors: Langhorst Jost , Koch Anna K. , Kehm Christina , Öznur Özlem , Engler Harald , Häuser Winfried
Published: April 2023

The challenging treatment situation of patients with fibromyalgia syndrome (FMS) requires additional therapy options. The effects of water-filtered infrared-A whole-body hyperthermia (WBH) versus sham hyperthermia on pain intensity were investigated in an outpatient setting within a two-armed randomized sham-controlled trial. n = 41 participants aged between 18 and 70 years with a medically confirmed diagnosis of FMS were randomized to WBH (intervention; n = 21) or sham hyperthermia (control; n = 20). Six treatments with mild water-filtered infrared-A WBH over a period of three weeks with at least one day in between treatments were applied. On average, the maximum temperature was 38.7 °C for a duration of approximately 15 min. The control group received exactly the same treatment except that an insulating foil between the patient and the hyperthermia device blocked most of the radiation. Primary outcome was pain intensity measured by the Brief Pain Inventory at week 4. Secondary outcomes included blood cytokine levels and FMS-related core symptoms and quality of life. Pain intensity at week 4 was significantly different between the groups in favor of WBH (p = 0.015). A statistically significant pain reduction in favor of WBH was also found at week 30 (p = 0.002). Mild water-filtered infrared-A WBH effectively reduced pain intensity at the end of treatment and follow-up.
Dental and Maxillofacial Emergency Algorithms in Swiss Emergency Departments
Authors: Sommacal Adelita , Bingisser Roland , Filippi Andreas , Bethke Mascha , Thieringer Florian M. , Jaquiéry Claude , Berg Britt-Isabelle
Published: April 2023

This study aimed to evaluate the availability and use of dental and maxillofacial emergency algorithms in Swiss hospitals. A survey was performed among physicians at Swiss emergency departments (ED) and participants of the “36th Annual Meeting of the Society for Oral and Cranio-Maxillofacial Surgery”. Eighty-nine EDs in Switzerland were questioned about the availability and use of electronic algorithms in their hospitals. Eighty-one (91%) participated in the study. In 75 (93%) of the EDs, electronic algorithms are used, mainly “medStandards”. Six have no available algorithms. Fifty-two (64%) use algorithms daily. Eight (10%) Swiss EDs have maxillofacial and dental algorithms, and 73 (90%) have no access to or do not know about them. For dental algorithms, 28 (38%) of the respondents would like to have access, and 16 (22%) do not desire access. For maxillofacial algorithms, 23 (32%) want to have access and 21 (29%) do not want it. Most (74%) of the participating maxillofacial surgeons did not know about the existence of ED algorithms regarding their specialty. Our study shows that the existence of specific algorithms is often not known. Furthermore, there is a demand for dental and maxillofacial algorithms in Swiss EDs.
Scalp Reconstruction Using the Latissimus Dorsi Free Flap: A 12-Year Experience
Authors: Strübing Felix , Wenz Fabian , Etminan Nima , Bigdeli Amir K. , Siegwart Laura C. , Thomas Benjamin , Vollbach Felix , Vogelpohl Julian , Kneser Ulrich , Gazyakan Emre
Published: April 2023

Background: Complex scalp defects are regularly reconstructed using microvascular tissue transfer. The latissimus dorsi free flap is one of the workhorse flaps used in scalp reconstruction. These cases necessitate, particularly in the elderly, a close cooperation between plastic surgeons and neurosurgeons. The purpose of this study was to evaluate the suitability of the latissimus dorsi free flap for complex scalp reconstructions and to analyze potential risk factors. Methods: A retrospective study identified 43 patients undergoing complex scalp reconstruction using a latissimus dorsi free flap at our department between 2010 and 2022. Results: The mean patient age was 61 ± 18 years. Defects were mostly caused by oncologic tumor resections (n = 23; 55%), exposure to a cranioplasty (n = 10; 23%) or infection (n = 4; 9%). The most frequent recipient vessels were the superficial temporal artery (n = 28; 65%), external carotid artery (n = 12; 28%) and the venae comitantes (n = 28; 65%), external jugular vein (n = 6; 14%). The reconstructive success rate was 97.7%. There was one total flap loss (2%). Partial flap loss occurred in five cases (12%). Follow-up was 8 ± 12 months. Major complications were seen in 13 cases, resulting in a revision rate of 26%. Multivariate logistic regression identified active tobacco use as the only risk factor for major complications (odds ratio 8.9; p = 0.04). Conclusion: Reconstruction of complex scalp defects using the latissimus dorsi free flap yielded high success rates. Among the potential risk factors, active tobacco use seems to affect the outcome of complex scalp reconstructions.
The Limitations of Anterior Knee Displacement during Different Barbell Squat Techniques: A Comprehensive Review
Authors: Illmeier Gabriel , Rechberger Julian S.
Published: April 2023

Based on seminal research from the 1970s and 1980s, the myth that the knees should only move as far anterior during the barbell squat until they vertically align with the tips of the feet in the sagittal plane still exists today. However, the role of both the hip joint and the lumbar spine, which are exposed to high peak torques during this deliberate restriction in range of motion, has remained largely unnoticed in the traditional literature. More recent anthropometric and biomechanical studies have found disparate results regarding anterior knee displacement during barbell squatting. For a large number of athletes, it may be favorable or even necessary to allow a certain degree of anterior knee displacement in order to achieve optimal training outcomes and minimize the biomechanical stress imparted on the lumbar spine and hip. Overall, restricting this natural movement is likely not an effective strategy for healthy trained individuals. With the exception of knee rehabilitation patients, the contemporary literature suggests it should not be practiced on a general basis.
4D-Flow Cardiovascular Magnetic Resonance Sequence for Aortic Assessment: Multi-Vendor and Multi-Magnetic Field Reproducibility in Healthy Volunteers
Authors: Punzo Bruna , Ranieri Brigida , Tramontano Liberatore , Affinito Ornella , Franzese Monica , Bossone Eduardo , Saba Luca , Cavaliere Carlo , Cademartiri Filippo
Published: April 2023

Objectives: Four-dimensional (4D) flow cardiac magnetic resonance (CMR) represents an emerging technique for non-invasive evaluation of the aortic flow. The aim of this study was to investigate a 4D-flow CMR sequence for the assessment of thoracic aorta comparing different vendors and different magnetic fields of MR scanner in fifteen healthy volunteers. Methods: CMR was performed on three different MRI scanners: one at 1.5 T and two at 3 T. Flow parameters and planar wall shear stress (WSS) were extracted from six transversal planes along the full thoracic aorta by three operators. Inter-vendor comparability as well as scan–rescan, intra- and interobserver reproducibility were examined. Results: A high heterogeneity was found in the comparisons for each operator and for each scanner in the six transversal planes analysis (Friedman rank-sum test; p-value ≤ 0.05). Among all, the most reproducible measures were extracted for the sinotubular junction plane and for the flow parameters. Conclusions: Our results suggest that standardized procedures have to be defined to make more comparable and reproducible 4D-flow parameters and mainly, clinical impactfulness. Further studies on sequences development are needed to validate 4D-flow MRI assessment across vendors and magnetic fields also compared to a missing gold standard.
Effect of Anthropometric Parameters on Achilles Tendon Stiffness of Professional Athletes Measured by Shear Wave Elastography
Authors: Römer Claudia , Zessin Enrico , Czupajllo Julia , Fischer Thomas , Wolfarth Bernd , Lerchbaumer Markus Herbert
Published: April 2023

Background: Shear wave elastography (SWE) is currently used to detect tissue pathologies and, in the setting of preventive medicine, may have the potential to reveal structural changes before they lead to functional impairment. Hence, it would be desirable to determine the sensitivity of SWE and to investigate how Achilles tendon stiffness is affected by anthropometric variables and sport-specific locomotion. Methods: To investigate the influence of anthropometric parameters on Achilles tendon stiffness using SWE and examine different types of sports to develop approaches in preventive medicine for professional athletes, standardized SWE of Achilles tendon stiffness was performed in 65 healthy professional athletes (33 female, 32 male) in the longitudinal plane and relaxed tendon position. Descriptive analysis and linear regression were performed. Furthermore, subgroup analysis was performed for different sports (soccer, handball, sprint, volleyball, hammer throw). Results: In the total study population (n = 65), Achilles tendon stiffness was significantly higher in male professional athletes (p < 0.001) than in female professional athletes (10.98 m/s (10.15–11.65) vs. 12.19 m/s (11.25–14.74)). Multiple linear regression for AT stiffness did not reveal a significant impact of age or body mass index (BMI) (p > 0.05). Subgroup analysis for type of sport showed the highest AT stiffness values in sprinters (14.02 m/s (13.50–14.63)). Conclusion: There are significant gender differences in AT stiffness across different types of professional athletes. The highest AT stiffness values were found in sprinters, which needs to be considered when diagnosing tendon pathologies. Future studies are needed to investigate the benefit of pre- and post-season musculoskeletal SWE examinations of professional athletes and a possible benefit of rehabilitation or preventive medicine.
Coronary Microvascular Dysfunction: Features and Prognostic Value
Authors: Kopeva Kristina , Grakova Elena , Maltseva Alina , Mochula Andrew , Gusakova Anna , Smorgon Andrew , Zavadovsky Konstantin
Published: April 2023

(1) Background: The results of the international studies support the assumption that coronary microvascular dysfunction (CMD) occurs significantly more often than previously identified and is associated with adverse outcomes. However, there is a lack of the accurate comprehension of its pathophysiology. The objectives of this study were to evaluate the clinical and instrumental features of CMD and to assess its prognostic value during 12 months of follow-up period. (2) Methods: A total of 118 patients with non-obstructive coronary artery disease (CAD) and preserved LV ejection fraction (62 [59; 64]%) were enrolled in the study. Serum levels of biomarkers were analyzed by enzyme-linked immunoassay. CMD was defined as the reduced myocardial flow reserve (MFR) ≤ 2 obtained by dynamic CZT-SPECT. Two-dimensional transthoracic echocardiography with evaluation of LV diastolic dysfunction was performed baseline. (3) Results: Patients were divided into groups depending on the presence of CMD: CMD+ group (MFR ≤ 2; n = 45), and CMD− group (MFR > 2; n = 73). In CMD+ group, the severity of diastolic dysfunction, the levels of biomarkers of fibrosis and inflammation were higher than in CMD− group. Multivariate regression analysis showed that the presence of diastolic dysfunction (OR 3.27; 95% CI 2.26–5.64; p < 0.001), the hyperexpression of NT-proBNP ≥ 760.5 pg/mL (OR 1.67; 95% CI 1.12–4.15; p = 0.021) and soluble ST2 ≥ 31.4 ng/mL (OR 1.37; 95% 1.08–2.98; p = 0.015) were independent factors associated with CMD. Kaplan–Meier analysis showed that a rate of the adverse outcomes was significantly (p < 0.001) higher in patients with CMD (45.2%, n = 19) than in patients without it (8.6%, n = 6). (4) Conclusions: Our data suggest that the presence of CMD was associated with the severe diastolic dysfunction and hyperexpression of the biomarkers of fibrosis and inflammation. Patients with CMD had higher rate of the adverse outcomes than those without it.
Molecular Variability of the Fusarium solani Species Complex Associated with Fusarium Wilt of Melon in Iran
Authors: Sabahi Fatemeh , Banihashemi Zia , Mirtalebi Maryam , Rep Martijn , Cacciola Santa Olga
Published: April 2023

Species of the Fusarium solani species complex (FSSC) are responsible for the Fusarium wilt disease of melon (Cucumis melo), a major disease of this crop in Iran. According to a recent taxonomic revision of Fusarium based primarily on multilocus phylogenetic analysis, Neocosmospora, a genus distinct from Fusarium sensu stricto, has been proposed to accommodate the FSSC. This study characterized 25 representative FSSC isolates from melon collected in 2009–2011 during a field survey carried out in five provinces of Iran. Pathogenicity assays showed the isolates were pathogenic on different varieties of melon and other cucurbits, including cucumber, watermelon, zucchini, pumpkin, and bottle gourd. Based on morphological characteristics and phylogenetic analysis of three genetic regions, including nrDNA internal transcribed spacer (ITS), 28S nrDNA large subunit (LSU) and translation elongation factor 1-alpha (tef1), Neocosmospora falciformis (syn. F. falciforme), N. keratoplastica (syn. F. keratoplasticum), N. pisi (syn. F. vanettenii), and Neocosmospora sp. were identified among the Iranian FSSC isolates. The N. falciformis isolates were the most numerous. This is the first report of N. pisi causing wilt and root rot disease in melon. Iranian FSSC isolates from different regions in the country shared the same multilocus haplotypes suggesting a long-distance dispersal of FSSC, probably through seeds.
A Conceptual Model to Share Resources and Align Goals: Building Blockchain Application to Support Care Continuity Outside a Hospital
Authors: Tanniru Mohan R. , Woo Carson , Dutta Kaushik
Published: April 2023

The increased use of advanced technologies by consumers and hospitals is moving care closer to patients, and the challenge is one of how patient data can be shared with external care providers and patients. To support care continuity, patient data include both clinical data used by external care providers and non-clinical data used by social care providers. Care coordination of a patient outside a hospital requires peer-to-peer connectivity among a number of these clinical and social care providers, using a digital platform that aligns their goals and assigns their resource sharing responsibilities. With no single entity supporting such care coordination, most hospitals currently distribute this responsibility to several of its provider partners and patients. Such a division of responsibility with no real time feedback leads to discontinuous resource sharing, localized data analysis, and challenges in tailoring care to improve health outcomes. The goal of this paper is to propose a blockchain architecture model that uses a number of constructs for creating and assigning ownership to patient data so it can support peer-to-peer resource sharing and uses smart contracts to support goal alignment. Using two blockchain applications implemented in Hyperledger and illustrating their potential representation using the constructs in multi-chain, we develop a conceptual model for developing blockchain applications in general to support continuity of care. The generalizability of this model is illustrated by applying these constructs to four additional healthcare applications. Finally, we conclude the paper with a discussion of the limitations and directions for future research.
Structural Amelioration of Soils for Sustainable Land Management
Authors: Tsapko Yurii , Kucher Anatolii , Meshref Bahaa , Krupin Vitaliy , Rozmarina Albina , Holovina Olesya , Skorokhod Iryna
Published: April 2023

The aim of this study is to determine the effects of structural soil restoration on the buffering capacities of these soils, their productivity, and the efficiency of their use as a basis for sustainable management. Based on a review of literature sources and our own experimental research, the proposed article shows the possibility of improving the buffering capacities of sod-podzolic cohesive sandy soils through the use of structural amelioration as an effective measure to protect them from degradation and ensure their resilience to climate change. The use of structural ameliorants (clay and peat) in the studied soils improves the granulometric composition, has a positive effect on the pH-buffering capacities, and contributes to optimizing the moisture capacity of soil. It was found that the efficiency of the application of structural amelioration on sod-podzolic cohesive sandy soils increases significantly with the local application method (e.g., clay in a dose of 10 t/ha or a combined application of clay in a dose of 2 t/ha with lowland peat in a dose of 3 t/ha). The largest yield increase in winter wheat (27.2%) was achieved by the local application of 2 t/ha of clay combined with peat in a dose of 3 t/ha.
Characteristics of Spatiotemporal Variations and Driving Factors of Land Use for Rural Tourism in Areas That Eliminated Poverty
Authors: Liu Yuanli , Liao Heping , Qiu Jiqing , Liu Yan
Published: April 2023

This study explores the spatiotemporal characteristics and driving factors of land use for rural tourism in areas that eliminated poverty from 2009 to 2021. It puts forward targeted governance measures to promote the high-quality development of rural tourism, poverty alleviation, and rural revitalization. The analysis is based on exploratory spatial analysis methods and geographical detectors. The results show that (1) the overall level of land use for rural tourism was low but grew very quickly with large regional differences. (2) There was a significant spatial agglomeration in land use for rural tourism land. The spatial distribution of land use for rural tourism landscapes was characterized by two cores and four clusters, while spatial distribution of rural tourism facilities was characterized by one cluster with multiple branches. (3) The driving factors of spatial variations in land use for rural tourism were diverse and dynamic. Dominant factors shifted from natural conditions and geographical location to socioeconomic and tourism resources and regional policy dimensions. Policy should emphasize the development of the rural tourism industry, innovate the diversified “tourism + development” model, enhance the level of land use for rural tourism, broaden avenues for farmers to increase their income, and strengthen residents’ motivation for development.
Bryorutstroemia (Rutstroemiaceae, Helotiales), a New Genus to Accommodate the Neglected Sclerotiniaceous Bryoparasitic Discomycete Helotium fulvum
Authors: Baral Hans-Otto , Sochorová Zuzana , Sochor Michal
Published: April 2023

The new genus Bryorutstroemia is established for the red-brown, stipitate, bryoparasitic discomycete Helotium fulvum Boud. Combined phylogenetic analysis of ITS and LSU rDNA and EF1α revealed that Bryorutstroemia fulva belongs to the sclerotiniaceous clade, which comprises the paraphyletic families Rutstroemiaceae and Sclerotiniaceae. Bryorutstroemia formed with Clarireedia a supported clade (Rutstroemiaceae s.l.), though with high distance. Bryorutstroemia closely resembles other Rutstroemiaceae in having uninucleate ascospores with high lipid content and an ectal excipulum of textura porrecta, but is unique because of its bryophilous lifestyle and is extraordinary with its thick-walled inamyloid ascus apex. Although B. fulva was described in 1897, very few records came to our notice. The present study summarizes the known distribution of the species, including 25 personal collections from the years 2001–2022. Bryorutstroemia fulva was most often encountered on Dicranella heteromalla, and rarely on other members of Dicranales or Grimmiales, while inducing necrobiosis of the leaves. A detailed description based on mainly fresh apothecia is provided together with a rich photographic documentation. Six new combinations are proposed based on our phylogenetic results and unpublished personal morphological studies: Clarireedia asphodeli, C. calopus, C. gladioli, C. henningsiana, C. maritima, and C. narcissi.
Fermented Vegetables and Legumes vs. Lifestyle Diseases: Microbiota and More
Authors: Knez Eliza , Kadac-Czapska Kornelia , Grembecka Małgorzata
Published: April 2023

Silages may be preventive against lifestyle diseases, including obesity, diabetes mellitus, or metabolic syndrome. Fermented vegetables and legumes are characterized by pleiotropic health effects, such as probiotic or antioxidant potential. That is mainly due to the fermentation process. Despite the low viability of microorganisms in the gastrointestinal tract, their probiotic potential was confirmed. The modification of microbiota diversity caused by these food products has numerous implications. Most of them are connected to changes in the production of metabolites by bacteria, such as butyrate. Moreover, intake of fermented vegetables and legumes influences epigenetic changes, which lead to inhibition of lipogenesis and decreased appetite. Lifestyle diseases’ feature is increased inflammation; thus, foods with high antioxidant potential are recommended. Silages are characterized by having a higher bioavailable antioxidants content than fresh samples. That is due to fermentative microorganisms that produce the enzyme β-glucosidase, which releases these compounds from conjugated bonds with antinutrients. However, fermented vegetables and legumes are rich in salt or salt substitutes, such as potassium chloride. However, until today, silages intake has not been connected to the prevalence of hypertension or kidney failure.
Regulation of Cell Proliferation and Nrf2-Mediated Antioxidant Defense: Conservation of Keap1 Cysteines and Nrf2 Binding Site in the Context of the Evolution of KLHL Family
Authors: Shilovsky Gregory A. , Dibrova Daria V.
Published: April 2023

Keap1 (Kelch-like ECH-associated protein 1) is one of the major negative regulators of the transcription factor Nrf2 (nuclear factor erythroid-2-related factor 2), which induces the expression of numerous proteins defending the cell against different stress conditions. Keap1 is generally negatively regulated by post-translational modification (mostly via its cysteine residues) and interaction with other proteins that compete with Nrf2 for binding. Cysteine residues in Keap1 have different effects on protein regulation, as basic residues (Lys, Arg, and His) in close proximity to them increase cysteine modification potential. In this paper, we present an evolutionary analysis of residues involved in both mechanisms of Keap1 regulation in the broader context of the KLHL protein family in vertebrates. We identified the typical domain structure of the KLHL protein family in several proteins outside of this family (namely in KBTBD proteins 2, 3, 4, 6, 7, 8, 12 and 14). We found several cysteines that are flanked by basic residues (namely, C14, C38, C151, C226, C241, C273, C288, C297, C319, and C613) and, therefore, may be considered more susceptible to regulatory modification. The Nrf2 binding site is completely conserved in Keap1 in vertebrates but is absent or located in nonaligned DA and BC loops of the Kelch domain within the KLHL family. The development of specific substrate binding regions could be an evolutionary factor of diversification in the KLHL protein family.
Heteropolyacid Ionic Liquid-Based MCF: An Efficient Heterogeneous Catalyst for Oxidative Desulfurization of Fuel
Authors: Pei Tingting , Chen Yaxian , Wang Huiting , Xia Lixin
Published: April 2023

A new type of catalyst was synthesized by immobilizing heteropolyacid on ionic liquid-modified mesostructured cellular silica foam (denoted as MCF) and applied to the oxidative desulfurization of fuel. The surface morphology and structure of the catalyst were characterized by XRD, TEM, N2 adsorption–desorption, FT-IR, EDS and XPS analysis. The catalyst exhibited good stability and desulfurization for various sulfur-containing compounds in oxidative desulfurization. Heteropolyacid ionic liquid-based MCF solved the shortage of the amount of ionic liquid and difficult separation in the process of oxidative desulfurization. Meanwhile, MCF had a special three-dimensional structure that was not only highly conducive to mass transfer but also greatly increased catalytic active sites and significantly improved catalytic efficiency. Accordingly, the prepared catalyst of 1-butyl-3-methyl imidazolium phosphomolybdic acid-based MCF (denoted as [BMIM]3PMo12O40-based MCF) exhibited high desulfurization activity in an oxidative desulfurization system. The removal of dibenzothiophene could achieve levels of 100% in 90 min. Additionally, four sulfur-containing compounds could be removed completely under mild conditions. Due to the stability of the structure, sulfur removal efficiency still reached 99.8% after the catalyst was recycled six times.
Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering
Authors: Zhe Man , Wu Xinyu , Yu Peiyun , Xu Jiawei , Liu Ming , Yang Guang , Xiang Zhou , Xing Fei , Ritz Ulrike
Published: April 2023

In recent years, three-dimensional (3D) bioprinting has been widely utilized as a novel manufacturing technique by more and more researchers to construct various tissue substitutes with complex architectures and geometries. Different biomaterials, including natural and synthetic materials, have been manufactured into bioinks for tissue regeneration using 3D bioprinting. Among the natural biomaterials derived from various natural tissues or organs, the decellularized extracellular matrix (dECM) has a complex internal structure and a variety of bioactive factors that provide mechanistic, biophysical, and biochemical signals for tissue regeneration and remodeling. In recent years, more and more researchers have been developing the dECM as a novel bioink for the construction of tissue substitutes. Compared with other bioinks, the various ECM components in dECM-based bioink can regulate cellular functions, modulate the tissue regeneration process, and adjust tissue remodeling. Therefore, we conducted this review to discuss the current status of and perspectives on dECM-based bioinks for bioprinting in tissue engineering. In addition, the various bioprinting techniques and decellularization methods were also discussed in this study.
Development, Processing and Aging of Novel Zn-Ag-Cu Based Biodegradable Alloys
Authors: Heiss Alexander , Thatikonda Venkat Sai , Richter Andreas , Schmitt Lisa-Yvonn , Park Daesung , Klotz Ulrich E.
Published: April 2023

The use of biodegradable materials for implants is a promising strategy to overcome known long-term clinical complications related to permanent implants. Ideally, biodegradable implants support the damaged tissue for a certain period and then degrade, while the physiological function of the surrounding tissue is restored. Although Mg-based alloys nearly ideally lend themselves to biodegradable implants, a few critical shortcomings promoted the development of alternative alloy systems. Due to their reasonably good biocompatibility, moderate corrosion rate without hydrogen evolution and adequate mechanical properties, increasing attention has been paid to Zn alloys. In this work, precipitation-hardening alloys in the system Zn-Ag-Cu were developed relying on thermodynamic calculations. After casting the alloys, their microstructures were refined by thermomechanical treatment. The processing was tracked and directed, respectively, by routine investigations of the microstructure, associated with hardness assessments. Although microstructure refinement increased the hardness, the material proved to be susceptible to aging as the homologous temperature of zinc is at 0.43 Tm. Besides mechanical performance and corrosion rate, long-term mechanical stability is another crucial factor that must be taken into consideration to ensure the safety of the implant and thus requires a profound understanding of the aging process.
Technological Processes for Increasing the Cavitation Erosion Resistance of Nimonic 80A Superalloys
Authors: Belin Cosmin , Mitelea Ion , Bordeașu Ilare , Crăciunescu Corneliu Marius , Uțu Ion-Dragoș
Published: April 2023

Nickel-based superalloys are frequently used to manufacture the components that operate under cavitation erosion conditions, such as aircraft gas turbine construction, nuclear power systems, steam turbine power plants, and chemical and petrochemical industries. Their poor performance in terms of cavitation erosion leads to a significant reduction in service life. This paper compares four technological treatment methods to improve cavitation erosion resistance. The cavitation erosion experiments were carried out on a vibrating device with piezoceramic crystals in accordance with the prescriptions of the ASTM G32—2016 standard. The maximum depth of surface damage, the erosion rate, and the morphologies of the eroded surfaces during the cavitation erosion tests were characterized. The results indicate that the thermochemical plasma nitriding treatment can reduce mass losses and the erosion rate. The cavitation erosion resistance of the nitrided samples is approximately 2 times higher than that of the remelted TIG surfaces, approximately 2.4 times higher than that of the artificially aged hardened substrate, and 10.6 times higher than that of the solution heat-treated substrate. The improvement in cavitation erosion resistance for Nimonic 80A superalloy is attributed to the finishing of the surface microstructure, graining, and the presence of residual compressive stresses, factors that prevent crack initiation and propagation, thus blocking material removal during cavitation stresses.
Microstructural Considerations of a Multi-Pass Rolled Ti-Nb-Ta-Zr Alloy
Authors: Răducanu Doina , Nocivin Anna , Cojocaru Vasile Dănuț , Șerban Nicolae , Zărnescu-Ivan Nicoleta , Irimescu Raluca Elena , Gălbinașu Bogdan Mihai
Published: April 2023

The microstructural characteristic evolution was investigated during thermomechanical processing of Ti-29Nb-9Ta-10Zr (wt %) alloy, which consisted of, in a first stage, in a Multi-Pass Rolling with increasing thickness reduction of 20%, 40%, 60%, 80%, and 90%; in step two, the multi-pass rolled sample with the highest thickness reduction (90%) was subjected to a series of three variants of static short recrystallization and then to a final similar aging. The objective was to evaluate the microstructural features evolution during thermomechanical processing (phase’s nature, morphology, dimensions, and crystallographic characteristics) and to find the optimal heat treatment variant for refinement of the alloy granulation until ultrafine/nanometric level for a promising combination of mechanical properties. The microstructural features were investigated by X-ray diffraction and SEM techniques through which the presence of two phases was recorded: the β-Ti phase and the α″-Ti martensitic phase. The corresponding cell parameters, dimensions of the coherent crystallite and the micro-deformations at the crystalline network level for both recorded phases were determined. The majority β-Ti phase underwent a strong refinement during the Multi-Pass Rolling process until ultrafine/nano grain dimension (about 9.8 nm), with subsequent slow growing during recrystallization and aging treatments, hindered by the presence of sub-micron α″-Ti phase dispersed inside β-Ti grains. An analysis concerning the possible deformation mechanisms was performed.
Numerical Simulation of Slag Entrainment by Vortex Flux during Tapping at Converter
Authors: Huang Chengyong , Sun Ye , Liu Wei , Li Jingshe , Yang Shufeng , Dong Jianfeng
Published: April 2023

In order to improve the yield of steel produced in the converter and the quality of the molten steel, and to understand the distribution of the flow field in the converter and ladle during the steelmaking process, the CFD fluid simulation software Fluent 2020 R2 was used to analyze the flow field of the converter static steelmaking process. The aperture of the steel outlet and the timing of the vortex formation under different angles were studied, as well as the disturbance level of the injection flow in the ladle molten pool. The study revealed that in the steelmaking process, the emergence of tangential vectors caused the entrainment of slag by the vortex, whereas in the later stages of steelmaking, the turbulent flow of slag disrupted the vortex, resulting in its dissipation. When the converter angle increases to 90°, 95°, 100°, and 105°, the eddy current occurrence time is 43.55 s, 66.44 s, 68.80 s, and 72.30 s, and the eddy current stabilization time is 54.10 s, 70.36 s, 70.95 s, and 74.26 s, respectively. When the converter angle is 100–105°, it is suitable to add alloy particles into the ladle molten pool. When the tapping port diameter is 220 mm, the eddy current inside the converter changes and the mass flow rate of the tapping port is “oscillating”. When the aperture of the steel outlet was 210 mm, the steelmaking time could be shortened by about 6 s without affecting the internal flow field structure of the converter.
Research on Waste Combustion in the Aspect of Mercury Emissions
Authors: Kijo-Kleczkowska Agnieszka , Gnatowski Adam , Tora Barbara , Kogut Krzysztof , Bytnar Krzysztof , Krzywanski Jaroslaw , Makowska Dorota
Published: April 2023

The topic of waste combustion/co-combustion is critical, given the increasingly restrictive legal regulations regarding its environmental aspects. In this paper, the authors present the test results of selected fuels of different compositions: hard coal, coal sludge, coke waste, sewage sludge, paper waste, biomass waste and polymer waste. The authors conducted a proximate and ultimate analysis of the materials and mercury content in them and their ashes. An interesting element of the paper was the chemical analysis of the XRF of the fuels. The authors conducted the preliminary combustion research using a new research bench. The authors provide a comparative analysis of pollutant emissions—especially mercury emission—during the combustion of the material; this is an innovative element of this paper. The authors state that coke waste and sewage sludge are distinguished by their high mercury content. The value of Hg emission during the combustion depends on the initial mercury content in the waste. The results of the combustion tests showed the adequacy of mercury release compared to the emissions of other compounds considered. Small amounts of mercury were found in waste ashes. The addition of a polymer to 10% of coal fuels leads to a reduction in mercury emissions in exhaust gases.
Preparation of Bis-Thiophene Schiff Alkali–Copper Metal Complex for Metal Corrosion Inhibition
Authors: Liu Yafei , Feng Huixia , Wang Luyao , Yang Tiantian , Qiu Jianhui
Published: April 2023

Due to the obvious numerous economic and technical consequences of the corrosion process, its inhibition is one of the most critical aspects of current research. A corrosion inhibitor for the bis-thiophene Schiff base copper–metal complex Cu(II)@Thy-2 was investigated here, which was synthesized via a coordination reaction with a bis-thiophene Schiff base (Thy-2) as a ligand and copper chloride (CuCl2-2H2O) as a ligand metal salt. When the corrosion inhibitor concentration was increased to 100 ppm, the self-corrosion current density Icoor reached a minimum of 2.207 × 10−5 A/cm2, the charge transfer resistance reached a maximum of 932.5 Ω·cm2, and the corrosion inhibition efficiency reached a maximum of 95.2%, with the corrosion inhibition efficiency showing a trend of increasing first and then decreasing with concentration increase. After adding Cu(II)@Thy-2 corrosion inhibitor, a uniformly distributed dense corrosion inhibitor adsorption film formed on the surface of the Q235 metal substrate, significantly improving the corrosion profile compared to both before and after the addition of the corrosion inhibitor. Before and after the addition of corrosion inhibitor, the metal surface’s contact angle CA increased from 54.54° to 68.37°, showing that the adsorbed corrosion inhibitor film decreased the metal surface’s hydrophilicity and increased its hydrophobicity.
Evaluation of the Number of Primary Grains in Hypoeutectic Chromium Cast Iron with Different Wall Thickness Using the ProCAST Program
Authors: Guzik Edward , Kopyciński Dariusz , Burbelko Andriy , Szczęsny Andrzej
Published: April 2023

The treatment of inoculation of white cast iron with carbide precipitations that consist of increasing the number of primary austenite grains is not as well-known as the treatment of inoculation of gray cast iron in which the number of eutectic grains increases. In the studies included in the publication, experiments were carried out using the addition of ferrotitanium as an inoculant for chromium cast iron. The Cellular Automaton Finite Elements (CAFE) module of ProCAST software was used in order to analyze the formation of the primary structure of hypoeutectic chromium cast iron in a casting of various thicknesses. The modeling results were verified using Electron Back-Scattered Diffraction (EBSD) imaging. The obtained results confirmed obtaining a variable number of primary austenite grains in the cross-section of the tested casting, which significantly affects the strength properties of the obtained chrome cast iron casting.