bluuebunny/crossref_metadata_2025 huggingface dataset:
Studies on Extraction Technique of Chitin from the Pupa of Dictyoploca Japonica and Quality Test of Product
Authors: Yang Bao Shan , Wang Hui , Li Jun , Liu Su
Published: September 2011

In order to produce high-quality chitin, the extraction of chitin from pupa of Dictyoploca japonica was studied by acid-alkali method. The extraction technique of chitin from the pupa was as the follows: Pupa skin were dipped in 4%HCl for 20h in 35°C , then in 6%NaOH for 4h in 85°C, followed by decoloring in 10% H2O2 for 0.5h under 85°C. Under this conditions, the produced chitin was white flaky solid with 6.5%-9.5% water content, 0.65%-0.97% ash content and 5.78%-6.67% nitrogen content, the productivity of the processing was 28%. Its quality indexes reached that of edible chitin.
A Practical Approach for Producing Hydrophobic and Elastic Aerogels by Ambient Pressure Drying
Authors: Cai Jackie Y. , Xu Bi , Wang Li Jing , Cao Yi , Finn Niall , Cai Zai Sheng
Published: September 2011

Hydrophobic and elastic aerogels derived from methyltrimethoxysilane (MTMS) precursor were prepared by ambient pressure drying under various processing conditions, and their morphology and physical and mechanical properties were characterised and evaluated. The results of this study demonstrated that for inherently hydrophobic gels like MTMS based gels, high temperature ageing is a practical and effective means to reduce volume shrinkage and produce low bulk density monolithic aerogels at ambient pressure, without the need of any solvent exchange and surface modification. By using this simple method, we were able to produce an aerogel with a bulk density as low as 0.064g/cm3. The results also revealed a significant difference from that previously reported in the literature.
Effects of Long-Term Elevated CO2 on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields
Authors: Jia Xia , Zhou Chun Juan
Published: September 2011

The effect of long-term elevated CO2(as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO2compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO2in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO2concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.
The Thermal and Rheological Properties of Starch Plasticized in Glycerol-Water Mixture
Authors: Liu Peng , Gu Cai Qin , Zeng Qing Zhu
Published: September 2011

The interaction between glycerol and water molecules is very complex, which displays different plasticization properties. In this paper, it studied the influence of glycerol-water mixture with different glycerol ratio on the thermal and rheological properties of starch. In water-rich mixture (glycerol ratio is less than 50%), its plasticization effect is similar as water, but will be impaired with the increase of glycerol. In glycerol-rich mixture (glycerol ratio is more than 90%), its lubrication and plasticization effect is similar as glycerol, and since glycerol can abstract lipid and destroy amylose-lipid complex, the peak M2 disappeared in DSC curves. In transition mixture, the mutual interactions reach maximal and less active hydroxy left, so the plasticization effect is the weakest, which is reflected by the highest loading peak and phase transition peak in torque-time curve by rheometer.
Effect of Endophytic Fungi on the Growth Situation in Cell Suspension Cultures of Acer Ginnala Maxim
Authors: Qi Feng Hui , Zhan Ya Guang , Zeng Fan Suo , Jing Tian Zhong
Published: September 2011

Fungal endophytes do not harm the plant when they live inside the host. Previous studies also showed that the content of some secondary metabolites are correlated to the endophytes within plant. Whether such a mutualistic symbiosis system could be implanted to bio-engineering or not will be an important and exciting problem. Here a fungal endophyte was co-cultured with the cell suspension of Acer ginnnala which is rich in gallich acid to test the problem above. The results showed that the growth of plant cells and the cell vitality decreased after the endophyte added, while gallic acid content increased. It was preliminarily determined that there is a partial competition relationship.
Preparation and Characterization of Egg Yolk Antibody against Staphylococcus Aureus
Authors: Peng Wei , Sun Yuan Ming , Liu Dan , Zhao Su Qing
Published: September 2011

Staphylococcus aureus is a pathogen responsible for purulent infection in humans and animals. To prevent disease of the nasopharynx, it is significant to prepare specific egg-yolk antibodies for determination establishment. In this paper, egg yolk antibody against staphylococcus aureus was prepared by immunization of laying hens with straphylococcus aureus as antigen. Egg yolk immunoglobulin (IgY) was isolated and purified from the egg yolks by polyethylene glycol 6000 and ammonia sulfate solution method. The titer and purify of IgY was evaluated by enzyme-linked immunosorbent assay (ELISA) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) respectively. Bacteriostatic effect was assayed with bacteriostatic test by plate method. Cross-reactivities with other bacterias were investigated by indirect ELISA. The specificity of IgY and antigen combination was assayed with agar-gel immunodiffusion method. The purity of IgY is 95.2 % and the titer is 1:5120. The IgY is with high specificity of streptococcus aureus. It can bind to staphylococcus aureus antigen well. The results indicate it provide a new approach to determine and prevent staphylococcus aureus.
Antioxidant Properties of Hot Water Extracts from Armillaria Mellea and Hohenbuehelia Serotina
Authors: Ji Hong Fang , Zhang Ling Wen , Zhang Hai Yan , Nan Hai Juan , Yang Da Guang , Yang Ming Duo
Published: September 2011

Hot water extracts from Armillaria mellea and Hohenbuehelia serotina fruit bodies were prepared and investigated for their antioxidant capacity in three different assays, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and chelating ability on ferrous ions. Among the two mushrooms hot water extracts, extract from A. mellea was better than that from H. serotina in scavenging ability and chelating effect on ferrous ions. With regard to reducing power, EC50 value of extracts from H. serotina was 0.447 mg/mL, while that of extracts from A. mellea was 1.914. From EC50 values obtained, it can be concluded that hot water extracts from A. mellea and H. serotina were good in antioxidant properties.
Growth Inhibition and Cell Cycle Arrest of Kappa-Selenocarrageenan and Paclitaxel on HepG2 Cells
Authors: Ling Na
Published: September 2011

To evaluate the anti-proliferative effect of kappa-selenocarrageenan (KSC) in combination with paclitaxel (Taxol) on human hepatoma cell line HepG2 and their synergistic effect. The cytotoxic effect of drugs on HepG2 cells was measured by MTT assay, and the King’s principle was used to evaluate the interaction of drug combination. Cell cycle and apoptotic rate were detected by flow cytometry (FCM). Expression of cyclin A, chk2, Cdc25A and cdk2 were detected by flow cytometry and western blotting. KSC and Taxol demonstrated growth inhibitory effect on HepG2 cells in a dose-dependent manner, with the IC50 values of Taxol decreased dramatically from 1.684 nmol/L to 0.272 nmol/L while combining with 30 mg/L KSC (P<0.01). In addition, additive activity was observed when 30 mg/L KSC was combined with Taxol (0.5~5 nmol/L) (q=0.85~1.15). FCM results showed that KSC and Taxol both blocked cell cycle in S phase and their combination enhanced S phase arrest. Western blotting analysis revealed that Cyclin A and chk2 protein expression in HepG2 cells increased but Cdc25A and cdk2 expression decreased.
The Expression and Characterization of Gp85 Gene of Subgroup J Avian Leukosis Virus Associated with Hemangioma
Authors: Shi Min , Lin Yan , Zhao Yang , Guo Ming Ping , Sun Luo Mei , Tian Ming Xing , Cao San Jie , Wen Xin Tian , Huang Yong
Published: September 2011

The subtype of avian leukosis virus (ALV) was mainly determined by the gp85 glycoprotein. A subtype J ALV strain SCDY1 associated with hemangioma was isolated from grandparent breeding chicken and the highly antigenic region of its gp85 gene was amplified and expressed in Rosetta Escherichia coli using the pET-32a(+)vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native gp85 protein as determined by Western blot assay using polyclonal antibodies against ALV-J strain. The fusion protein was also purified. This research lays a foundation for using this recombinant protein for development of indirect enzyme-linked immunosorbent assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent ALV-J.
Inulin in the Application of Bio-Energy
Authors: Zhao Chun Hai
Published: September 2011

This article summarizes the development of current condition of bio-energy development simply, including the most bio-ethanol and biodiesel are potential, but the material is the biggest obstacle. Inulin is present as a reserve carbohydrate in the roots and tubers of plants,which will maybe use to ethanol fermentation, single cell oil production and inulooligosaccharide(IOS) production.
Effects of Nutritional Factors on Production of Bacterial Bioactive Metabolites against Aflatoxin Biosynthesis
Authors: Wang Zhuo , Yan Pei Sheng , Cao Li Xin , Li Qian Wei , Lin Feng
Published: September 2011

Bacillus subtilis is potential to play an important role on biological control of aflatoxin-producing fungi. The liquid fermentation medium of Bacillus subtilis LN for production of bioactive metabolites inhibitory to mycelial growth and aflatoxin biosynthesis was optimized by single factor experiments. The results showed that optimum carbon source was glucose, the optimum nitrogen source was soy peptone, and the optimum inorganic salt was MgSO4.These screened optimal carbon, nitrogen and inorganic salt sources could be used for high yield fermentation of bioactive metabolites for developing biopesticide against aflatoxin contamination in foods.
Surface Modification of Poly(Vinylidene Fluoride) Microporous Membrane by Ferric Hydroxide Sol-Gel
Authors: Xie Ya Jie , Hu Wan Peng , Wang Zhuan , Xu Song
Published: September 2011

Surface modification of poly(vinylidene fluoride) (PVDF) membrane was accomplished by the adsorption of ferric hydroxide sol to impove its hydrophilicity. The adsorption curves of the membranes were analyzed by weight measurements, and the typical results showed a two-platform character in low concentration. The effects of the adsorption time, the ferric hydroxide sol concentration, the test temperature, as well as the water cleaning time on the adsorption amount were observed. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR/ FTIR) analysis showed that the adsorption of ferric hydroxide sol on poly(vinylidene fluoride) microporous membranes (PVDFMM) is effective. The membranes hydrophilic performance was represented by water contact angle measurements. The water contact angles changes from the virgin 128°to the lowest value 81.90° in the range of 0~0.10 mol/L ferric hydroxide aqueous solution. The results indicated that ferric hydroxide sol can be immobilized on the PVDF films by adsorption, and the membrane hydrophilic performence increases with the increase of the adsorption amount of the ferric hydroxide sol onto the PVDF membrane surface.
Effect of Levels of Yucca Schidigera Extract on Ruminal Fermentation Parameters, Digestibility of Nutrients and Growth Performance in Sheep
Authors: Liu Chun Long , Li Zhong Qiu
Published: September 2011

An in vivo fermentation experiment (Expt.1) and a digestibility and growth trial(Expt.2) were conducted to determine the effect of levels of Yucca Schidigera Extract (YSE) on ruminal fermentation parameters ,digestibility of nutrients and growth performance in Sheep. Three same levels of YSE supplementation were studied in both experiments. In the Expt.1,four rumen fistulated male sheep with initial body weight of 33±1.8 ㎏were randomly assigned according to a 4×4 Latin square design. The dietary treatments were YSE based offering at 0,100, 200 and 300 mg/kg diet. It was found that ruminal pH was not significantly different among treatments. Relative to control, ruminal propionate concentration was increased by YSE addition in a dose-dependent manner by up to 29.79%（P<0.05）,the acetic concentration was decreased by up to2 1.23%（P<0.05）.Ruminal ammonia concentration was larger (P<0.05) in sheep receiving no YSE (increased by 18.86 mg/dl) than in those receiving 200mg/kg (2.85 mg/dl increase in NH3) or 300mg/kg (2.72 mg/dl increase). Protozoa populations in the rumen were lower (P<0.05) with YSE of 200-300 mg/kg than without. In the digestibility and growth trial(Expt.2),increasing levels of YSE resulted in liner increase in daily body weight gain rate (P<0.05) and feed conversion efficiency(P<0.05). Additionally ,apparent digestibilities(%) of DM,OM,CP,and NDF were significantly different in all treatment .In conclusion, the 200 and 300 mg/kg YSE groups have a particular suppressing effect on ruminal ammonia concentration, ammonia N concentrations and protozoa populations were decreased, The effects on ruminal propionate and acetic concentration were probably a result of a selective inhibitory effect of YSE on rumen microbial species. The effect of YSE on ruminal ammonia concentration likely resulted from a decreased population of protozoa, presumably, from ammonia binding by YSE. YSE can significantly improved the apparent digestibility of nutrient and sheep growth performance.
Statics Analysis of the Mechanical Model of Nucleosome
Authors: Xue Yun , Weng De Wei , Gong Gang Ming
Published: September 2011

Mechanical model of nucleoside and its equilibrium equations are set up, and the mechanical properties on the equilibrium position are analyzed. In the case constraint force and electrostatic attraction between cylinder OH and elastic rod are balanced, the analytic expression of nutation angle of the section and its conditions of existence are given. It is show that the cylinder OH can maintain equilibrium at any range of the precession angle. In the other case when unbanced, there is phenomenon of separation of elastic rod from cylinder OH in the spiral wound 2 circles, and numerical solution of the precession angle at separation points are calculated. Analysis of equilibrium of cylinder H1 illustrates that the generatrix of cylinder H1 and OH are not parallel, and the angle between them is obtained
Immobilization of Phosphorus-Dissolving Bacteria on Sawdusts for Removing Phosphorus in Iron Ores
Authors: Huang Fa Ming , Xia Shui Chun , Xiao Xin Tong , Luo Bin Hua , Xia Shi Bin , Gong Wen Qi
Published: September 2011

Nowadays, the immobilization of At.t is a research potential. The immobilization of At.t on the sawdust filler was discussed in this paper. The experimental results indicated that sawdusts, as the immobilization fillers, can provide a stable living microenvironment for At.t showing the favorable bioactivity and strong acid-producing capacity. Also, under the optimal conditions with the inoculums concentration 12.5%, sulphur dosage 1% (w/v), dissolved oxygen (DO) in the bioreactor above 6 mg/l, the conductivity, pH, and H+ in the bioreactor were 45.2 ms/cm，0.964, and 0.109 mol/l，respectively. In the leaching ore experiment within 24 hours, bacteria effect of phosphorus removal was unconspicuous.
Identiﬁcation of Translationally Optimal Codons and Suitable Expression Host of DPV gB Gene
Authors: Jiang Long , Cheng An Chun , Wang Ming Shu , Zhu De Kang , Jia Ren Yong
Published: September 2011

In this report, we conduct study on codon composition and codon usage of DPV glycoprotein B (gB) gene, its homologs constitute the most highly conserved family of herpesvirus glycoproteins and are present in members of each herpesvirus subfamily. Our results show that sixty-one codons (excluding the termination codons) in the polypeptide, a high level of diversity in codon usage bias existed for coding the Ala, Gly, Leu, Pro, Arg, Ser, Thr and Val amino acids. Sixteen codons (each for a amino acid), including GCA (Ala), GAT (Asp), GAA (Glu), GGA (Gly), CAT (His), ATA (Ile), AAA (Lys), CTA (Leu), AAT (Asn), CCA (Pro), CAA (Gln), AGA (Arg), TCT (Ser), ACT (Thr), GTA (Val) and TAT (Tyr) were determined as the translationally optimal codons. The codon preferences of DPV gB gene were compared with those of E. coli, yeast, and H. sapiens, we can speculate that the DPV gB gene may be more efﬁciently expressed in the E. coli system. In summary, knowledge of codon usage of herpesvirus gB genes provides insights into molecular and species evolution, and also plays important role in furthering some biotechnological applications. These would be fruitful areas for further study.
Effect of Steam Explosion Pretreatment and Microbial Fermentation on Degradation of Corn Straw
Authors: Chang Juan , Yin Qing Qiang , Ren Tian Bao , Song An Dong , Zuo Rui Yu , Guo Hong Wei
Published: September 2011

The effects of steam explosion (2.5 MPa, 200s) and Trichoderma koningii (T. koningii) fermentation on corn straw degradation were evaluated according to straw degradation and enzyme activity in the fermented products. The results showed that the steam explosion pre-treatment for corn straw could reduce the contents of cellose, hemicellulose and lignin by 8.47%, 50.45% and 36.65%, respectively (P<0.05). After the pretreated corn straw with steam explosion was fermented by T. koningii for 6 days, the contents of cellulose and hemicellulose in the fermented straw were decreased by 19.37% and 63.54%, compared with the original corn straw (P<0.05); decreased by 11.83% and 26.41%, compared with the exploded straw (P<0.05). The filter paper cellulase, CMCase and amylase protease protease protease;activities in the fermented products were 356.39,5 599.90 and 834.00 U/g, respectively. It is concluded that the corn straw pre-treated by steam explosion and followed by T. koningii fermentation for 6 days seems to be a new prospective method for corn straw degradation and application.
Coupling Law Research of Single Phase Stress Field / Seepage Field in Reservoir Deformation Medium
Authors: Wu Jun Xian
Published: September 2011

On the basis of summarizing the predecessors' achievements，according to the basic theory of rock mechanics, fluid mechanics in porous medium, geologic mechanics, calculated mechanics and fluid-solid coupling seepage, a mathematical and numerical model of seepage field and stress field coupling is developed in porous medium and the LiuNan blocks of JiDong oilfield wells.based area single phase Stress Field / Seepage Field in solving with the method of finite element software ANSYS, Contrast analysis of LiuNan blocks Coupling around of Field / Seepage Field is the change of average stress and total displacement. As can be seen from Table 3, after coupling the value of average stress and total displacement are greater than before coupling.So from figure 2 know than it is important meaning for used to solve practical engineering problems.
Role of Ethylene in the Biosynthesis of Fatty Acid-Derived Volatiles in Tomato Fruits
Authors: Xie Yuan Hong , Gao Hong Yan , Luo Yun Bo , Zhang Hong Xing , Chen Xiang Ning , Zhu Ben Zhong
Published: September 2011

Regulation of ethylene biosynthesis or action has an important effect on volatiles production in tomato (Lycopersicon esculentum) fruits. To understand the role of ethylene in the biosynthesis of fatty acid-derived aroma volatiles in tomato, we used Lichun tomato from a transgenic line with strictly suppression of ethylene biosynthesis (antisenseLeACS2tomato) and its wild type background line. This study was focused on the levels of the precursor substrates, activities and transcriptional levels of aroma volatile-related enzymes, including lipoxygenase (LOX), hydroperoxide lyase (HPL) and alcohol dehydrogenase (ADH). We also investigated the different abilities of converting the precursor substrates to aroma volatiles in ethylene suppressed transgenic and wild-type (WT) tomato fruits. Our results showed that the contents of endogenous linoleic and linolenic acid in tomato fruits were ethylene depended. Suppression of ethylene biosynthesis increased the content of endogenous linolenic acid inLichuntomato fruit and then declined the ratio of linoleic /linolenic acid. Exogenous ethylene changed the value of linoleic acid /linolenic acid in antisenseLeACS2(ACS) tomato fruit to the similar level of WT. During the ripening of wild type Lichun tomato fruit, LOX activity was ethylene and development dependent. Suppression of ethylene biosynthesis did not inhibit the transcriptional expression ofLoxCgene. And the HPL and ADH activities were partial ethylene-dependent during the ripening of wild typeLichuntomato fruit. Moreover, suppression of ethylene biosynthesis also affected the bioconversion of unsaturated-fatty acid precursors to C6 aldehydes and C6 alcohols. All these results indicated that ethylene had complicated effects on the biosynthesis of fatty acid-derived armoa volatiles by affecting the precursor’s content, enzyme activities, enzyme expression and the substrate utilization.
Morphogenesis-Associated/Dissociated Production of Epothilone in Wild Sorangium Cellulosum Strains
Authors: Liu Xin Li , Zhao Lin , Hu Wei , Li Yue Zhong
Published: September 2011

To investigate the characters ofSorangiumstrains and the approaches for improving the production of epothilone, correlation of epothilone production and morphogenesis was studied in strains So0157-2, So0007-3andSo0003-3. On lean medium, So0157-2 could produce epothilones in a wide pH range (5.0-12.0), but formed fruiting bodies at high pH values (9.0-14.0). Formation of fruiting body did not affect the yield of epothilones in So0157-2. In So0007-3 and So0003-3, producing of epothilone was strongly coupled with the process of morphogenesis. It suggests that reduction of the association between morphogenesis and epothilone producing would prominently improve the epothilone production.
Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes
Authors: Li Feng Juan , Wang Chang Lu , He Dong , Liu Ya Qiong , Chen Mian Hua , Wang Yu Rong , Li Feng Juan , Yang Zhao Hui , Chen Grace
Published: September 2011

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.
Antioxidation Study of Pholiota adiposa (Fr.) Quel and its Polysaccharides
Authors: Gong Chun Yu , Hu Qing Xiu , Ji Ye Mei
Published: September 2011

.Four kinds of water-soluble polysaccharides A1(from fruit body), A2(from mycelia), PA(purification polysaccharide extracted from fruit body) and A6(from fermentation broth), which were isolated from an edible mushroom Pholiota.adiposa (Fr ) Quel and its aqueous extract, fruit body power and mycelia power of the P. adiposa were studied in antioxidation experiment. They were all found to have the ability of scavenging free radicals, but their effect on O2-• and •OH were different. A6 had the best oxyradical scavenging activity and PA was the best in scavenging hydroxyl radical.
Numerical Simulation Software Design on Protein Particle Detection
Authors: He Peng , Zhou Zhen , Wang Lian Peng , Wang Na
Published: September 2011

Light scattering approach and optical measuring instrument are the most popular system in the protein aggregation detection, especially in the therapeutic and diagnostic protein industry. Microsoft windows based software written in Matlab was developed as a teaching/research tool for designing light particle instrument and detection systems. This software incorporates information for lots of optical parameters, formulas and methods. There is a choice among 3 light scattering system design style. The software output includes advising parameter of light particle instrument and detection system, intensity of light scattering at some angle, characteristic curves of light scattering style. The software also provides the capability of visualizing the design layout and performs numerical computation to analyze the experimental data.
EUK1111 Attenuates Scopolamine-Induced Learning Deficits in the Passive Avoidance Test
Authors: Zeng Qing Wen , Duan Ya Le , Cao Xiao Hua , Tao Bai Rui
Published: September 2011

The aim of the experiment was to investigate the effects of the EUK1111 on passive avoidance of amnestic mice model induced by scopolamine. Five groups were divided: Control, model, model + high concentration (260 mg/kg), model + middle concentration (104.2 mg/kg) and model + low concentration (5.2 mg/kg). An open field experiment was conducted to test the locomotor activity after EUK1111 was taken orally 30 days. Scopolamine was injected intraperitoneal (1.5 mg/kg ) 10 minutes before passive avoidance test was conducted. There were no significant difference among the groups on move time, total moves, move velocity and move distance (P > 0. 05). However, in passive avoidance test, the latencies to dark side were significantly longer in model + high concentration group, model + middle concentration group than model group ( P < 0. 05). It indicates that EUK1111 may attenuate scopolamine induced learning deficits.
[D-Ala2, D-Leu5]-Enkephalin (Dadle) Reduces Inflammation Responses after Transient Global Ischemia
Authors: Wang Shu Yan , Duan Ya Le , Zeng Qing Wen , Zhao Zheng , Wang Xiang Rui
Published: September 2011

[D-Ala2, D-Leu5]-Enkephalin (DADLE) is a δ-opioid receptor antagonist and has been shown to reduce neuronal loss in the selectively vulnerable brain regions after transient global ischemia. Here, we investigate whether this protection is mediated by the DADLE's modulation of the postischemia inflammation responses. After implanted with cannula at the right lateral ventricle, rats underwent 10 minutes of transient global ischemia by four vessel occlusion. Rats received a single infusion of DADLE (12.5 nmol) via the intracerebral cannula at the onset of reperfusion. At the time of 72 h after ischemia, we investigated GFAP expression via immunohistochemistry. we also tested the level of MDA and the activities of SOD and CAT. The results show that DADLE can reduce reactive astrocytosis and increase SOD activities. The study reveals the neuroprotection mechanism of DADLE in the ischemic brain is related to reduce inflammation responses.
The in Silicon Cloning ILF2 Gene and Prediction of ILF2 Protein Structure in Sheep
Authors: Sun Ying Ning , Zhao Yu , Wang Wei Yu
Published: September 2011

In silicon cloning, we obtained ILF2 gene by using human ILF2 gene sequence (NM_004515) to be probe. Sequence analysis showed that the in silicon cloned cDNA was 1662 base pairs long with an open reading frame (ORF) containing 1173 nucleotides encoding a protein of 390 amino acids. 5’-untranslated region (UTR) was 74 bp, and 3’-UTR was 413 bp. A comparison of the sheep ILF2 with cow, horse, human, mouse, xenopus and zebra fish ILF2 amino acids had 96%, 91%, 91%, 81%, 61%, and 54% identity. The PI was 5.19, and molecular weight of the deduced protein was 43 050.12 Da. The pig ILF2 contained a RGG-rich single-stranded RNA-binding domain and a DZF zinc-finger nucleic acid binding domain. This study laid a foundation for further analysis of structure, expression and regulation of ILF2 gene in sheep.
Application of FEM Simulation Technology on Vibration Reliability Analysis of Electronic Components
Authors: Song Fang Fang , He Xiao Qi , En Yun Fei
Published: September 2011

Vibration capability and reliability is necessarily to be considered for electronic components design. In this paper, the vibration characteristics of electronic components were studied using FEM, for example, gridded electron gun. The dynamical mode of girded electron gun was established. Modal frequency and model obtained by simulation shows tendency as tested experiment results, verifying the correctness of the simulation model and method used. The Parameters showed an inherent property of structure, which provided a theoretical basis for production design, the optimization of parameters and the vibration-resistance reliability improvement of microwave tube. In the aspects of electronic components reliability analysis, the variations of production capability parameters under various stress environments can be gained by FEM simulation technology to evaluate environment adaptability and reliability.
Investigation and Simulation on Amorphous Formation and Growth
Authors: Li Ning , Chen Wei Zeng
Published: September 2011

The amorphous formation mechanism was studied from the viewpoint of the crystallography. The experimental results showed that the heterogeneity atoms with chemical bonds had a higher capability of forming into compounds than that of congener atoms. It was proposed that the banded structure was induced by the variation of active atoms with a proper thickness, alternated depletion and enrichment of anions in the diffusion layer due to generation and evolution of hydrogen gas. The chemical force plays an important part in the formation. The factors working on chemical reaction affect the formation in the same. A number of steady motes are in a high speed movement, and collide with each other in all probability. The formation of amorphous alloys is the result of massive nucleus appearance, which was proposed from the crystallography in this paper. The cause of the chaotic distribution is of the suppression of each nucleus growth. The short-range order atomic structure is of the atomic nucleus growth orientation. And the long-range disorder structure is of the massive nucleus suppression growth.
Effect of Internal Electrical Field on the Electrical Breakdown Properties of Contact Materials
Authors: Liu Jun Ru , Xiao Peng , Wang Xian Hui , Liang Shu Hua , Liu Han Yang
Published: September 2011

In order to get a deep understanding of the effect of internal electrical field on the electrical breakdown properties of contact materials, two kinds of W-Cu and Cr-Cu joints were prepared in a vacuum sintering furnace. The vacuum breakdown tests were respectively performed at the interface of two joints and pure metal ends in an arc extinguishing chamber, and the surface morphologies after electrical breakdown 50 times were characterized by a scanning electron microscopy equipped with an energy dispersive spectroscopy. The results show that the breakdown strength at the interface is much larger than that of pure metal ends, and the breakdown site deviates from the interface. It is suggested that the existence of internal electrical field at the interface of two contact metals changes the electronic structure, and, thus, the electrical breakdown behavior is influenced.
Vacuum Arc Motion Characteristics on CuWCr Composites
Authors: Xiao Peng , Liu Jun Ru , Wang Xian Hui , Liang Shu Hua , Liu Han Yang
Published: September 2011

The CuWCr composites were respectively fabricated by the mixture of W and Cr and WCr alloyed powders, followed by sintering and infiltration. Vacuum arcs on CuWCr composites were observed by a digital high-speed video camera. The morphologies of the composites before and after arc erosion were characterized by a scanning electron microscope. The results show that the microstructure CuWCr composites prepared by WCr alloyed powders becomes finer and more uniform, and, thus, has good characteristics of diffusive arcs and arc erosion resistance. In addition, the composite prepared by WCr alloyed powders has excellent electrical properties such as high breakdown voltage, low chopping current and long arc life. Arc erosion zones of the CuWCr composite fabricated by WCr alloyed powders become more dispersive and uniform on the surfaces with some shallow erosion pits. During the course of arc evolution, the dispersed arcs are caused by the split of electrical arc.
The Role of Nano-SiO2 Addition in the Mechanical Properties of RHA Composite Cement Mortars
Authors: Sadrmomtazi Ali , Fasihi A.
Published: September 2011

This paper presents a laboratory study on the properties of rice husk ash (RHA) cement composite mortars incorporating nano-SiO2. Different amounts of nano-SiO2 (0%, 1%, 3% and 5%) were incorporated into the mortars with 20% replacement of RHA. The compressive and flexural strengths of mortars were tested at 7, 28, 60 and 90 days. The water absorption test carried out at 28 days. The drying shrinkage of mortars was measured up to the age of 42 days. Scanning electron microscopy (SEM) observation also conducted to evaluate the effect of nano-SiO2 on microstructure of cement paste containing RHA. Incorporating nano-SiO2 into RHA mortars significantly improved the mechanical strength and water absorption properties of the products. Nano-SiO2 increased the drying shrinkage of RHA mortars. SEM analysis showed that nano-SiO2 improved the microstructure of RHA pastes on dense and compact form.
Design and Analysis on Dynamic Characteristics of Grinding Machine for High-Pressure Vessel of Nuclear Power
Authors: Huang Zhi , Huang Yun , Liu Ying , Wang Ya Jie , Zhang Yue
Published: September 2011

In the dynamic design of 6 axes high efficient grinding machine for high-pressure vessel of nuclear power, the paper established the 3D solid digital model of grinding machine based on parametric design criterion by Pro/E, and calculated the result of static and vibration model analysis on the whole machine with the ANSYS, preliminary grasped the dynamic and static characteristics of the grinding machine, these were very essential for us to optimize the grinding machine structure and follow-up to manufacture successfully.
Effect Factors Analysis of Flow Noise Generation from Perforated Duct Muffler Element
Authors: Zhao Hai Jun , Deng Zhao Xiang
Published: September 2011

Flow noise regeneration from perforated tube muffler element was measured on the self-developing test bench, relationship model on total sound power of flow regeneration noise and structure parameters and work condition was established. Its model parameters were solved making use of hyper static least square method. Using the model effect factors of flow noise generation were discussed. Result shows that the reducing of the perforated diameter and the perforated part length is favor of the reduction of flow noise, and perforated ratio and expansion chamber diameter have less effect on flow noise. After analyzing spectrum structure of flow regeneration noise, it is displayed that with the increase of flow velocity projected peak value frequency has the trend of moving to middle and high, its intensity also becomes stronger, and sound energy in some the frequency accounts for about 60% of the total energy, Strophe number is the range of from 0.2 to 0.35.
Fast Automatic Matching Method on Multi-View Point Clouds for Rail Tanker
Authors: Fu Tao , Liu Wei Jun , Zhao Ji Bin
Published: September 2011

In this paper, a novel method is proposed for automatically matching the measured data of a rail tanker. The spindle orientation of point cloud is determined by PCA, and then any relative point pair is well matched according to the spatial topology relations as the complexity reduction point of view, finally the data is mapped to a uniform coordinate system with the Quaternion method. The experimental results demonstrated that the proposed method achieves simplicity of implementation with robustness, and especially suitable for positioning the work piece of the rail tanker and the multi-view data fusion.
Research on Aero Engine Blade Information Model Based on Manufacturability Evaluation
Authors: Fan Qing Ming , Cao Yan , Liu Hong Jun
Published: September 2011

According to the engineering demands of the manufacturability evaluation, the Aero engine blade part information model built up based on the generalized feature and STEP standard, and based on the secondary development of UG software, a CAD system based on features is realized. The system can realize automatic feature recognition. Based on the part information model that has been built up, the system can generate STEP document automatically, can provide various blade part information for the subsequent manufacturability evaluation of the design. This helps to realize the integration and parallel of CAD and the subsequent process. The CAD/CPAPP/DFM information integration can be realized by using the blade part information that has been built up.
Research on the Relation of Brands Image to Product Form
Authors: Wang Yan Qun , Zhang Bing Chen , Guo Wei Min
Published: September 2011

Brands can help companies achieve their growth objectives more quickly and more profitably. Product form is the main consideration of customers purchase. As one intact products design, not only must we standardize and set restrictions in concerning production, sales and marketing and design itself, but also have to provide different product form for different brands to some extent. Reserve these product form features in the products should establish the company’s product form style so that the product form is distinguishable, unique, thus set up corporate image to keep the brand loyalty of consumers.
Collision Detection of Building Facility Pipes and Ducts Based on BIM Technology
Authors: Han Ning , Yue Zi Feng , Lu Yuan Fang
Published: September 2011

As construction space becomes increasingly constrained, the need to avoid collision among various HVAC, sewage and electronic pipes has become a pressing concern for modern building engineering projects. This article uses Building Information Modeling(BIM) technology to construct a three-dimensional piping model and utilizes Autodesk Navisworks software to integrate project data from various operators in order to pinpoint potential conflicts locations and generate a comprehensive ducting collision detection report. The advantages of this virtual construction vehicle include improving engineering, designing quality and efficiency.
The Influence of Glass Types on the Performance of Air-Conditioned Office Buildings in Australia
Authors: Guan Lisa
Published: September 2011

Windows are one of the most significant elements in the design of buildings. Whether there are small punched openings in the facade or a completely glazed curtain wall, windows are usually a dominant feature of the building's exterior appearance. From the energy use perspective, windows may also be regarded as thermal holes for a building. Therefore, window design and selection must take both aesthetics and serviceability into consideration. In this paper, using building computer simulation techniques, the effects of glass types on the thermal and energy performance of a sample air-conditioned office building in Australia are studied. It is found that a glass type with lower shading coefficient will have a lower building cooling load and total energy use. Through the comparison of results between current and future weather scenarios, it is identified that the pattern found from the current weather scenario would also exist in the future weather scenario, although the scale of change would become smaller. The possible implication of glazing selection in face of global warming is also examined. It is found that compared with its influence on building thermal performance, its influence on the building energy use is relatively small or insignificant.
Parametric Optimization Design on Mine Carrier Robot
Authors: Wei Juan , Ma Hong Wei
Published: September 2011

To a virtual prototype technology for tools，to a movement stationary of the carrier robot for goal, studied on the parametric optimization method based on virtual prototype technology. The paper designed the carrier robot mechanical structure and established the robot parameterization model in Solid Works, divided the robot into twelve rigid bodies, sixteen hinges and two elementary forces. Transferred the rigid bodies models into Universal Mechanism, added the bound hinges and the elementary forces, set the track irregularity model and path model. By computer simulations technology, took the wheelbase for example, introduced the selection method of the best value. The method can be applied to make certain the other parameters of the robot.
Embodying of Human-Centered Perspective in Household Basic Appliances Product Design
Authors: Guo Ting Liang , Liu Jun , Dai Hong Juan , Zhang Hui Xia
Published: September 2011

Traditional critical goals for industrial products design includes five factors. They are utility, appearance, ease of maintenance, low costs and communication[1]. In this paper, puts forth a new perspective, in which considers that embodying of human-centered perspective should be also another critical goal to industrial product besides traditional five critical goals. The paper is aimed at giving rise to the industrial designers to pay more attention to the design principles of ‘Human-Centered’ during their household bisic appliances product design because they are usually ignored by the designers. In the paper, mainly analyzed some household basic appliances products, which are used in every day life of people. The way of study is to analyze some problems in the products design by the examples of the products that are being applied in every day life, but there are some defects. What the analyses mainly concerned is how to embody the principle of “human-centered” in industrial product design. The “green design” and “healthy design” should be put the first place when industrial designers to design industrial products.
Virtual Shaping Design of Battery Car in the Golf
Authors: Du Qiu Lei , Cheng Xian Chun
Published: September 2011

Shaping design of battery car in the golf is a comprehensive design, which involves many fields such as engineering technology, art shaping, man-machine engineering and so on. In this paper, the overall design, graphics design and virtual shaping design of battery car in the golf have been discussed; the four stages including preparation, idea, improvement and virtual manufacturing about shaping design of battery car have been analyzed.
Load Spectrum Test and Numerical Simulation on the Rolling Rack for Dynamic Design and Control
Authors: Zhao Wu , Huang Dan
Published: September 2011

The sustained rolling mill vibration on high-speed cold rolling mill or smooth mill could cause that periodicity sheet thickness difference or board light and dark stripes, or rolling processing interruption for the large vibration intensity. Dynamic load behavior of the rolling mill rack including the load magnitude and load property usually was the reason of the vibration, which caused the vibration modal with diversity and uncertainty. Three-dimensional solid modeling software and finite element analysis software had been used to realize computer simulation on deformation and stress field calculation of the rack rolling mill for completing rolling processing performance evaluation of the plate rolling mill. By strain gauges being layout to be half-bridge circuit in its suitable location to detect the one-direction strain on the rack, the loading capacity of rolling mill’s rack was studied for its dynamic characterization behavior. By extracting rolling force signal on actual measured and statistical analyzing calculations of experimental data on the plate rolling mill, rolling force load spectrum was obtained. Analysis model on rack was established in order to calculate and analyze mechanical and power parameters based on finite element method. It was used for improving dynamic design, also for analyzing mechanical and power parameters matching with increasing production and product variety under rolling schedule reinforcement. This study work provide theoretical foundation on ensuring stable operation of equipment, which was benefits on increasing production and product variety, even rolling processing potential scope.
Protective Performance of the Operator's Helmet for Wheel Loader in the Rollover Accident
Authors: Si Jun De , Wang Guo Qiang , Zhang Ying Shuang , Hao Wan Jun
Published: September 2011

This paper is devoted to study of the helmets which can provide protection for the operator against head injury during an rollover accident. In the present study, the proposed procedure is demonstrated with the help of a validated madymo model of a featureless Hybrid Ⅲ headform in conjunction with a helmet model comprising an outer polypropylene shell to the inner surface of which is bonded a protective polyurethane foam padding of a given thickness, a parametric analysis has been performed in order to investigate the effect of different densities and thickness of the polyurethane foam on the head injury during impact. Based on simulation results of impact on a rigid surface, it appears that a minimum foam padding thickness of 15 mm is necessary for obtaining an acceptable value of HIC(d). The HIC(d) value decreases as an increase of the foam thickness, and the effect of the foam density variable is nonlinear, a second order parabolic shape can be identified.
Research of Ultrasonic Detection for Bonding Quality of Thin Plate Based on Fuzzy Pattern Recognition
Authors: Wang Xiao Juan , Zhang Ze , Wang Hai Tao
Published: September 2011

The application of fuzzy pattern recognition to ultrasonic detection for bonding quality of thin composite plate is studied. In this paper, firstly, the fuzzy membership function between each characteristic and bonding quality is established by BP neural network. Simulation results show that this method is convenient, simple, and it conforms to the practical application. Accordingly，the fuzzy subsets of standard modules and unknown bonding quality modules are established. Secondly, the fuzzy pattern recognition algorithm, which is designed by the nearest principle as the judging standard to judge bonding quality, is given. Experimental results show that the algorithm is very exact for quantitative recognition of bonding quality.
The Piezoelectric Constant Testing System Based on the Principle of Laser Scanning Fabry-Perot Interferometer
Authors: Tong Yue , Xia Zheng Rong , Ma Jian Guo , Lu Xiao Yong
Published: September 2011

The principles of measuring tiny deformation by scanning Fabry-Perot interferometer have been studied. The measuring method of piezoelectric constant testing based on laser scanning Fabry-Perot principle was proposed. This measuring method was verified through contrasting the measured values with the known ones of piezoelectric constant and the result showed the errors between them were less than 6%