bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation and Performance Study of Si-DLC Based on Ion Deposition of Different Multiple Gradient Transition Layers
Authors: Guo Ziming , Wang Renxin , Yang Hu , Chen Junrong , Lin Rongchuan , Wei Shasha , Li Bo
Published: June 2022

In order to improve the lower adhesion strength of Si-DLC coatings to the substrate and enhance its wear resistance, Si-DLC coatings with different transition layers (AlTiSiN, AlCrN and AlTiCrN) were prepared on 304 austenitic stainless steel substrates using multi-arc ion plating technique. The effects of different transition layers on the properties of Si-DLC were characterized by scanning electron microscopy, X-ray diffractometer, nanoindentation, confocal microscopy, Rockwell hardness tester, Raman test and wear test to find the optimal Si-DLC transition layer. The results show that the Si-DLC coating with AlTiSiN as the transition layer has an ID/IG of 0.71, the highest hardness of 26.7 Gpa, low surface roughness, the highest compressive stress, the best bond strength and the best wear resistance.
Simulation and Design of HgSe Colloidal Quantum-Dot Microspectrometers
Authors: Wen Chong , Zhao Xue , Mu Ge , Chen Menglu , Tang Xin
Published: June 2022

In recent years, colloidal quantum dots (CQD) have been intensively studied in various fields due to their excellent optical properties, such as size-tunable absorption features and wide spectral tunability. Therefore, CQDs are promising infrared materials to become alternatives for epitaxial semiconductors, such as HgCdTe, InSb, and type II superlattices. Here, we report a simulation study of a microspectrometer fabricated by integrating an intraband HgSe CQD detector with a distributed Bragg reflector (DBR). Intraband HgSe CQDs possess unique narrowband absorption and optical response, which makes them an ideal material platform to achieve high-resolution detection for infrared signatures, such as molecular vibration. A microspectrometer with a center wavelength of 4 µm is studied. The simulation results show that the optical absorption rate of the HgSe CQD detector can be increased by 300%, and the full-width-at-half-maximum (FWHM) is narrowed to 30%, realizing precise regulation of the absorption wavelength. The influence of the incident angle of light waves on the microspectrometer is also simulated, and the results show that the absorption rate of the HgSe quantum dot detector is increased 2–3 times within the incident angle of 0–23 degrees, reaching a spectral absorption rate of more than 80%. Therefore, we believe that HgSe CQDs are a promising material for realizing practical HgSe microspectrometers.
Participation of Rossiulus kessleri (Diplopoda, Julida) in the Formation of Algae Assemblages of Urbanized Territories
Authors: Pakhomov Olexandr , Pokhylenko Alevtyna , Maltseva Iryna , Kulbachko Yurii
Published: June 2022

Epi- and endozoochory are well explored phenomena that contribute to the distribution patterns of plant seeds, spores or fruits by vertebrates. It is less known how soil algae may be redistributed due to analogous interactions. We describe the participation of saprophage Rossiulus kessleri (Julidae, Diplopoda) in soil algae redistribution. The research was carried out in a park area of a large industrial megacity in the Dnipro steppe region (Ukrainian North Steppe subzone), Dnipro city. Research material was collected according to zoological and algological methods. The leaf litter of tree species (ash maple Acer negundo, Italian poplar Populus deltoides, locust Robinia pseudoacacia, European white elm Ulmus laevis, Norway maple Acer platanoides) and living diplopods were collected within 1 m2 quadrats along transects. Millipedes were maintained without feeding for five days, after which they were fed with the litter collected previously. Identification of algoflora was conducted in washes from the surface of diplopods’ bodies, its gut washes, in diplopods’ excretions and leaf litter washes. In the leaf litter, as R. kessleri’s feeding base, 14 soil algae species were identified—Nostoc punctiforme, Bracteacoccus minor, Mychonastes homosphaera, Neospongiococcum sp., Chlamydomonas sp., Chlorella vulgaris, Stichococcus bacillaris, Pseudococcomyxa simplex, Desmococcus olivaceus, Trebouxia spp., Klebsormidium flaccidum, Nephrodiella phaseolus, Navicula pelliculosa and Vischeria magna. In body surface washes, five soil algae species were identified, in gut washes seven algae species were found, while in excretions, just four were observed. It was established that not all algae species from the gut washes of R. kessleri were present in excretions. The presence of some representatives of soil algae, for example, Chlorella vulgaris and Vischeria magna, in natural park litter, body surface washes, gut washes and in excretions of R. kessleri, suggests that the given species pass through the diplopod’s gut and stay undamaged. This indicates that soil saprophage R. kessleri contributes to the dispersal of some soil algae representatives through epi- and endozoochory. Our results represent novel contributions to the knowledge of zoochory. It was shown that animals can be involved in dispersal of not only plant parts but also entire organisms, although it is unknown at what scale diplopods contribute to the diversity and dispersal of algae.
Correction: Günlü, O. Function Computation under Privacy, Secrecy, Distortion, and Communication Constraints. Entropy 2022, 24, 110
Authors: Günlü Onur
Published: June 2022

Special case results given in Lemmas 1–4 and evaluated in Section 4 [...]
Improved Sampled Average Modulation Technique for the Modular Multilevel Converters
Authors: Colque Juan , Filho Ernesto , Azcue José
Published: June 2022

In this article the improved sampled average modulation technique is proposed, this technique has 2N + 1 levels in the output voltage waveform of MMC and it is considered as of low complexity of implementation for any number of submodules per arm. For that, characteristics such as dynamic response, implementation complexity, inverter output voltage waveform levels, and switching frequency are considered to evaluate and validate the proposed modulation technique. The proposed technique is compared with other three previously proposed techniques, its considering parameters such as the THD and the fundamental value of the output voltage, and also the peak-to-peak variation of the submodule capacitor voltage. Several simulations were performed in the Matlab/Simulink software and with these results, it was validated the proposed modulation technique, and also it is verified that the proposed technique is computationally more efficient. This last one shows its potential for multiphase multilevel applications.
Predicting Rare Earth Element Potential in Produced and Geothermal Waters of the United States via Emergent Self-Organizing Maps
Authors: Engle Mark A. , Nye Charles W. , Neupane Ghanashyam , Quillinan Scott A. , McLaughlin Jonathan Fred , McLing Travis , Martín-Fernández Josep A.
Published: June 2022

This work applies emergent self-organizing map (ESOM) techniques, a form of machine learning, in the multidimensional interpretation and prediction of rare earth element (REE) abundance in produced and geothermal waters in the United States. Visualization of the variables in the ESOM trained using the input data shows that each REE, with the exception of Eu, follows the same distribution patterns and that no single parameter appears to control their distribution. Cross-validation, using a random subsample of the starting data and only using major ions, shows that predictions are generally accurate to within an order of magnitude. Using the same approach, an abridged version of the U.S. Geological Survey Produced Waters Database, Version 2.3 (which includes both data from produced and geothermal waters) was mapped to the ESOM and predicted values were generated for samples that contained enough variables to be effectively mapped. Results show that in general, produced and geothermal waters are predicted to be enriched in REEs by an order of magnitude or more relative to seawater, with maximum predicted enrichments in excess of 1000-fold. Cartographic mapping of the resulting predictions indicates that maximum REE concentrations exceed values in seawater across the majority of geologic basins investigated and that REEs are typically spatially co-associated. The factors causing this co-association were not determined from ESOM analysis, but based on the information currently available, REE content in produced and geothermal waters is not directly controlled by lithology, reservoir temperature, or salinity.
Experimental Assessment of the Impact of Replacing Diesel Fuel with CNG on the Concentration of Harmful Substances in Exhaust Gases in a Dual Fuel Diesel Engine
Authors: Karczewski Mirosław , Szamrej Grzegorz , Chojnowski Janusz
Published: June 2022

The problem of global warming and related climate change, as well as rising oil prices, is driving the implementation of ideas that not only reduce the consumption of liquid fuels, but also reduce greenhouse gas emissions. One of them is the use of natural gas as an energy source. It is a hydrocarbon fuel with properties allowing the reduction of CO2 emissions during its combustion. Therefore, solutions are being implemented that allow natural gas to be supplied to means of transport, which are trucks of various categories and purposes. This article presents the results of tests of an engine from a used semi-truck, to which an innovative compressed natural gas (CNG) supply system was installed. This installation (both hardware and software), depending on the engine operating conditions, enables mass replacement by natural gas (up to 90%) of the basic fuel—diesel oil. During the tests, on the basis of the obtained results, the influence of the diesel fuel/CNG exchange ratio under various engine operating conditions on the concentration of toxic CO2, CO, NO, NO2, CH4, C2H6, NMHC, NH3 and exhaust smoke was assessed. The test results confirm that, compared to conventional fueling, the diesel/CNG-fueled engine allows for a significant reduction in CO2 concentration even in a car operated for several years with diesel fuel and with high mileage. The use of a non-factory installation significantly increased the concentration of methane CH4, nitrogen dioxide NO2 and carbon monoxide CO in the exhaust gas. It was found that the smoke content and the temperature of exhaust gases did not decrease with increasing ratio of fuel replacement. The concentration of CO, NOX, CH4 and NMHC was increased, while the concentration of CO2, C2H6, NH3 and the consumption of diesel fuel by the engine, decreased significantly. The innovation of the research is based on the use of a modern and unique engine gas fuel system control system where the original fuel supply system with unit pumps is able to reduce diesel oil consumption by up to 90%.
Cooling Systems of Power Semiconductor Devices—A Review
Authors: Górecki Krzysztof , Posobkiewicz Krzysztof
Published: June 2022

In this paper, a detailed review of contemporary cooling systems of semiconductor devices is presented. The construction and the principles of operation of selected components of passive and active cooling systems, as well as selected computer tools supporting the design of such systems, are described. The dependences of thermal parameters calculated using these tools on selected factors characterizing the used cooling systems, e.g., the dimensions of their components, are presented and discussed. Additionally, some results of measurements illustrating the influence of selected parameters on the thermal resistance of power MOSFETs mounted in different cooling systems are shown. The properties of selected cooling systems are compared, and it is shown that by changing the type of cooling system, it is possible to reduce the thermal resistance value of a power MOSFET even 20 times. The presented considerations can make the process of designing cooling systems more effective.
Discovery of Rickettsia and Rickettsiella Intracellular Bacteria in Emerald Ash Borer Agrilus planipennis by Metagenomic Study of Larval Gut Microbiome in European Russia
Authors: Vecherskii Maxim V. , Orlova-Bienkowskaja Marina J. , Kuznetsova Tatyana A. , Bieńkowski Andrzej O.
Published: June 2022

Emerald ash borer Agrilus planipennis (Coleoptera: Buprestidae) is a quarantine pest posing a threat to ash trees all over Europe. This wood-boring beetle native to Asia is quickly spreading in North America and European Russia, and approaching the European Union and the Middle East. It is important to study microorganisms associated with this pest, because the knowledge of its “natural enemies” and “natural allies” could be potentially used for the control of the pest. All previously published information about the A. planipennis microbiome was obtained in North America and China. We present the first study on procaryotes associated with A. planipennis in Europe. Alive larvae were sampled from under the bark of Fraxinus pennsylvanica in the Moscow Oblast and the gut microbiome was studied using metagenomic methods. Next-generation Illumina-based amplicon sequencing of the v3-v4 region 16S-RNA gene was performed. In total, 439 operational taxonomic units from 39 families and five phyla were detected. The dominant families in our samples were Pseudomonadaceae, Erwiniaceae and Enterobacteriaceae, in accordance with the published information on the larval gut microbiome in North America and China. We detected intracellular bacteria in A. planipennis for the first time, namely Rickettsia (Rickettsiaceae) and Rickettsiella (Diplorickettsiaceae). Representatives of the genus Rickettsia are known to be in mutualistic symbiosis with some phytophagous insects, while Rickettsiella bacteria are pathogenic to many arthropods. The finding of Rickettsia and Rickettsiella opens perspectives for future research on the interactions between these bacteria and A. planipennis and the possible use of these interactions for the control of the pest.
Remembering Natalya Nikolaevna Vygodskaya
Authors: Schulze Ernst-Detlef , Tchebakova Nadezhda , Groisman Pavel , Oltchev Alexander , Panferov Oleg , Kurbatova Julia
Published: June 2022

As the Guest Editor I want to dedicate this Special Issue in memory of my university professor Natalya Nikolaevna Vygodskaya (Figure 1) [...]
First Steps of Asthma Management with a Personalized Ontology Model
Authors: Ajami Hicham , Mcheick Hamid , Laprise Catherine
Published: June 2022

Asthma is a chronic respiratory disease characterized by severe inflammation of the bronchial mucosa. Allergic asthma is the most common form of this health issue. Asthma is classified into allergic and non-allergic asthma, and it can be triggered by several factors such as indoor and outdoor allergens, air pollution, weather conditions, tobacco smoke, and food allergens, as well as other factors. Asthma symptoms differ in their frequency and severity since each patient reacts differently to these triggers. Formal knowledge is selected as one of the most promising solutions to deal with these challenges. This paper presents a new personalized approach to manage asthma. An ontology-driven model supported by Semantic Web Rule Language (SWRL) medical rules is proposed to provide personalized care for an asthma patient by identifying the risk factors and the development of possible exacerbations.
Cell Subsampling Recovers Probative DNA Profile Information from Unresolvable/Undetectable Minor Donors in Mixtures
Authors: Huffman Kaitlin , Hanson Erin , Ballantyne Jack
Published: June 2022

When a minor DNA component to a binary mixture is present at a weight ratio of approximately 1:50 or less, the presence of this minor donor is undetectable (or barely detectable) by standard mixture deconvolution approaches. In an attempt to retrieve probative minor donor DNA profile information, multiple quintuple cell subsamples were collected from a 1:50 DNA mixture using direct single cell subsampling (DSCS) paired with probabilistic genotyping (PG), the latter validated for use with single or few cells. DSCS employs a simplified micromanipulation technique paired with an enhanced DNA profiling approach, involving direct cell lysis and a sensitive PCR process, to genotype individual cells. Multiple five-cell subsamples were used to interrogate sufficient cells from the mixture such that some of the created 5-cell “mini-mixture” subsamples contained a cell from the minor donor. The latter mini-mixture subsamples, which now comprised weight ratios of 1:4 as opposed to the bulk mixture 1:50, were analyzed with the PG systems STRmixTM and EuroForMix resulting in a significant probative gain of information, (LR ≅ 1011, compared to standard bulk mixture PG methods, LR ≅ 101–102).
Effect of High-Volume Training on Psychological State and Performance in Competitive Swimmers
Authors: Chortane Oussama Gaied , Amara Sofiene , Barbosa Tiago M. , Hammami Raouf , Khalifa Riadh , Chortane Sabri Gaied , van den Tillaar Roland
Published: June 2022

This study aimed to examine the effect of four weeks of aquatic high-volume training (HVT) on the psychological state (somatic, cognitive anxiety, and self-confidence) and sprint swimming performance (50 m front crawl) compared to the standard training program (moderate volume training) in competitive swimmers. Twenty-eight male competitive swimmers participated in this study and were randomly allocated into two groups: HVT group (n = 14; age = 16.4 ± 0.31 years) and control group that underwent the standard training program (n = 14; age = 16.1 ± 0.30 years). All psychological state variables and swimming performance were measured in pre and post-test. Our findings showed a significant increase in anxiety state (34.13% to 45.83%; ES = 3.26 to 3.38) and a significant decrease in self-confidence (18.43%; ES = 2.39) after four weeks of HVT, while all psychological state variables remained unchanged in the control group (p > 0.05). In addition, our results showed no significant enhancement in swimming performance in both groups (p > 0.05). The sudden increase in training mileage negatively affected the anxiety, decreasing the state of self-confidence of the participants. In addition, four weeks of high training volume are insufficient to improve swimming performance. To conclude, gradually increasing the volume of the training load may be an adequate solution to promote adaptation to the effort, thus maintaining the stability of the psychological state of swimmers. In addition, it is recommended to integrate a concurrent mental preparation program with high-volume training to monitor the psychological state of competitive swimmers.
Exploring the Psychological Impacts of COVID-19 Social Restrictions on International University Students: A Qualitative Study
Authors: Al-Oraibi Amani , Fothergill Lauren , Yildirim Mehmet , Knight Holly , Carlisle Sophie , O’Connor Mórna , Briggs Lydia , Morling Joanne R. , Corner Jessica , Ball Jonathan K. , Denning Chris , Vedhara Kavita , Blake Holly
Published: June 2022

The global COVID-19 pandemic has impacted on the mental well-being of university students, but little attention has been given to international students, who may have a unique experience and perspective. The aim of this study was to explore the views of international students and university staff towards COVID-19 restrictions, self-isolation, their well-being, and support needs, through eight online focus groups with international students (n = 29) and semi-structured interviews with university staff (n = 17) at a higher education institution in England. Data were analysed using an inductive thematic approach, revealing three key themes and six subthemes: (1) practical, academic, and psychological challenges faced during self-isolation and the COVID-19 pandemic; (2) coping strategies to self-isolation and life during the pandemic; and (3) views on further support needed for international students. International students faced practical, academic, and psychological challenges during the COVID-19 pandemic, particularly relating to the rapid transition to online learning and the impact of social restrictions on integration with peers and well-being. Online social connections with peers, family, or new acquaintances reduced feelings of isolation and encouraged involvement in university life. Despite raising mental health concerns, most international students did not access mental health support services. Staff related this to perceived stigma around mental health in certain cultural groups. In conclusion, international students experienced specific practical and emotional challenges during the pandemic, and are at risk of mental ill-health, but may not actively seek out support from university services. Proactive and personalised approaches to student support will be important for positive student experiences and the retention of students who are studying abroad in the UK higher education system.
Influence of Adding Low Concentration of Oxygenates in Mineral Diesel Oil and Biodiesel on the Concentration of NO, NO2 and Particulate Matter in the Exhaust Gas of a One-Cylinder Diesel Generator
Authors: Maes Rafael R. , Potters Geert , Fransen Erik , Van Schaeren Rowan , Lenaerts Silvia
Published: June 2022

Air quality currently poses a major risk to human health worldwide. Transportation is one of the principal contributors to air pollution due to the quality of exhaust gases. For example, the widely used diesel fuel is a significant source of nitrogen oxides (NOx) and particulate matter (PM). To reduce the content NOx and PM, different oxygenated compounds were mixed into a mineral diesel available at the pump, and their effect on the composition of exhaust gas emissions was measured using a one-cylinder diesel generator. In this setup, adding methanol gave the best relative results. The addition of 2000 ppm of methanol decreased the content of NO by 56%, 2000 ppm of isopropanol decreased NO2 by 50%, and 2000 ppm ethanol decreased PM by 63%. An interesting question is whether it is possible to reduce the impact of hazardous components in the exhaust gas even more by adding oxygenates to biodiesels. In this article, alcohol is added to biodiesel in order to establish the impact on PM and NOx concentrations in the exhaust gases. Adding methanol, ethanol, and isopropanol at concentrations of 2000 ppm and 4000 ppm did not improve NOx emissions. The best results were using pure RME for a low NO content, pure diesel for a low NO2 content, and for PM there were no statistically significant differences.
Effects of Free Play and Partly Structured Playground Activity on Motor Competence in Preschool Children: A Pragmatic Comparison Trial
Authors: Tortella Patrizia , Haga Monika , Lorås Håvard , Fumagalli Guido Francesco , Sigmundsson Hermundur
Published: June 2022

Both the indoor and the outdoor environments and their organization exert pronounced influence upon physical activity behavior and motor development of preschool children. The aim of this study was to explore whether partly structured activity or free play in a specific playground had different impacts on motor competence development in 4–6-year-old preschoolers. The study had a pretest–post-test design, with two intervention groups and one control. Sixty-two children were included in a partly structured activity group and forty-three children in a free-play group. Both groups participated in playground activities consisting of 10 sessions (once a week), each lasting 1 h, in a specific playground setting. For the partly structured activity group, activities in each session consisted of a combination of both structured and free activity while the free-play group were engaged in unstructured play only. The control group did not attend the playground activities (N = 36). To assess levels of motor skills, each child completed pre- and post-tests using the Movement Assessment Battery for children (MABC-2), the Test of Motor Competence (TMC) and two playground-specific tests. A 3 (study group) and X 2 (gender) ANCOVAs were conducted on post-test scores on each of the test items from TMC, MABC-II and playground tests, with pre-test scores as covariates. Post hoc pairwise multiple comparisons were conducted with the alpha Bonferroni corrected, and the partial eta-squared (η2p) was applied as a measure of effect size. The results indicate no significant differences in motor competence measured by the TMC or the MABC-2 between groups. On the contrary, a significant improvement in performance in the playground-specific tests was observed in the partly structured activity group compared to the free-play and control groups.
Mobile Application Use and Loneliness among Older Adults in the Digital Age: Insights from a Survey in Hong Kong during the COVID-19 Pandemic
Authors: Yang Chun , Lai Daniel W. L. , Sun Yi , Ma Chun-Yin , Chau Anson Kai Chun
Published: June 2022

Existing literature on the associations between use of mobile applications (i.e., mobile apps) and loneliness among older adults (OAs) has been mainly conducted before the outbreak of the COVID-19 pandemic. Since mobile apps have been increasingly used by OAs during the pandemic, subsequent effects on social and emotional loneliness need updated investigation. This paper examines the relationship between mobile app use and loneliness among Hong Kong’s OAs during the pandemic. In our research, 364 OAs with current use experience of mobile apps were interviewed through a questionnaire survey conducted during July and August 2021, which assessed the use frequency and duration of 14 mobile app types and levels of emotional and social loneliness. The survey illustrated communication (e.g., WhatsApp) and information apps were the most commonly used. Emotional loneliness was associated with the use of video entertainment (frequency and duration), instant communication (duration), and information apps (duration). Association between video entertainment apps’ use and emotional loneliness was stronger among older and less educated OAs. Our findings highlight the distinctive relationships between different types of apps and loneliness among Hong Kong’s OAs during the pandemic, which warrant further exploration via research into post-pandemic patterns and comparative studies in other regions.
Bone Tissue Engineering in Rat Calvarial Defects Using Induced Bone-like Tissue by rhBMPs from Immature Muscular Tissues In Vitro
Authors: Hayashi Tatsuhide , Asakura Masaki , Kawase Mayu , Matsubara Masakazu , Uematsu Yasuaki , Mieki Akimichi , Kawai Tatsushi
Published: June 2022

This study aimed to induce bone-like tissue from immature muscular tissue (IMT) in vitro using commercially available recombinant human bone morphogenetic protein (rhBMP)-2, rhBMP-4, and rhBMP-7, and then implanting this tissue into a calvarial defect in rats to assess healing. IMTs were extracted from 20-day-old Sprague-Dawley (SD) fetal rats, placed on expanded polytetrafluoroethylene (ePTFE) with 10 ng/μL each of rhBMP-2, BMP-4, and BMP-7, and cultured for two weeks. The specimens were implanted into calvarial defects in 3-week-old SD rats for up to three weeks. Relatively strong radiopacity was observed on micro-CT two weeks after culture, and bone-like tissue, comprising osteoblastic cells and osteoids, was partially observed by H&E staining. Calcium, phosphorus, and oxygen were detected in the extracellular matrix using an electron probe micro analyzer, and X-ray diffraction patterns and Fourier transform infrared spectroscopy spectra of the specimen were found to have typical apatite crystal peaks and spectra, respectively. Furthermore, partial strong radiopacity and ossification were confirmed one week after implantation, and a dominant novel bone was observed after two weeks in the defect site. Thus, rhBMP-2, BMP-4, and BMP-7 differentiated IMT into bone-like tissue in vitro, and this induced bone-like tissue has ossification potential and promotes the healing of calvarial defects. Our results suggest that IMT is an effective tissue source for bone tissue engineering.
CAR T Cell Therapy in Hematological Malignancies: Implications of the Tumor Microenvironment and Biomarkers on Efficacy and Toxicity
Authors: Tan Jing Yuan , Low Muhammed Haiqal , Chen Yunxin , Lim Francesca Lorraine Wei Inng
Published: June 2022

Chimeric antigen receptor (CAR) T cell therapy has ushered in a new era in cancer treatment. Remarkable outcomes have been demonstrated in patients with previously untreatable relapsed/refractory hematological malignancies. However, optimizing efficacy and reducing the risk of toxicities have posed major challenges, limiting the success of this therapy. The tumor microenvironment (TME) plays an important role in CAR T cell therapy’s effectiveness and the risk of toxicities. Increasing research studies have also identified various biomarkers that can predict its effectiveness and risk of toxicities. In this review, we discuss the various aspects of the TME and biomarkers that have been implicated thus far and discuss the role of creating scoring systems that can aid in further refining clinical applications of CAR T cell therapy and establishing a safe and efficacious personalised medicine for individuals.
Neuronal-Immune Cell Units in Allergic Inflammation in the Nose
Authors: Klimov Vladimir , Cherevko Natalia , Klimov Andrew , Novikov Pavel
Published: June 2022

Immune cells and immune-derived molecules, endocrine glands and hormones, the nervous system and neuro molecules form the combined tridirectional neuroimmune network, which plays a significant role in the communication pathways and regulation at the level of the whole organism and local levels, in both healthy persons and patients with allergic rhinitis based on an allergic inflammatory process. This review focuses on a new research paradigm devoted to neuronal-immune cell units, which are involved in allergic inflammation in the nose and neuroimmune control of the nasal mucociliary immunologically active epithelial barrier. The categorization, cellular sources of neurotransmitters and neuropeptides, and their prevalent profiles in constituting allergen tolerance maintenance or its breakdown are discussed. Novel data on the functional structure of the nasal epithelium based on a transcriptomic technology, single-cell RNA-sequencing results, are considered in terms of neuroimmune regulation. Notably, the research of pathogenesis and therapy for atopic allergic diseases, including recently identified local forms, from the viewpoint of the tridirectional interaction of the neuroimmune network and discrete neuronal-immune cell units is at the cutting-edge.
Pharmacological Effects of Polyphenol Phytochemicals on the Intestinal Inflammation via Targeting TLR4/NF-κB Signaling Pathway
Authors: Yu Caiyun , Wang Dong , Yang Zaibin , Wang Tian
Published: June 2022

TLR4/NF-κB is a key inflammatory signaling transduction pathway, closely involved in cell differentiation, proliferation, apoptosis, and pro-inflammatory response. Toll like receptor 4 (TLR4), the first mammalian TLR to be characterized, is the innate immune receptor that plays a key role in inflammatory signal transductions. Nuclear factor kappa B (NF-κB), the TLR4 downstream, is the key to accounting for the expression of multiple genes involved in inflammatory responses, such as pro-inflammatory cytokines. Inflammatory bowel disease (IBD) in humans is a chronic inflammatory disease with high incidence and prevalence worldwide. Targeting the TLR4/NF-κB signaling pathway might be an effective strategy to alleviate intestinal inflammation. Polyphenol phytochemicals have shown noticeable alleviative effects by acting on the TLR4/NF-κB signaling pathway in intestinal inflammation. This review summarizes the pharmacological effects of more than 20 kinds of polyphenols on intestinal inflammation via targeting the TLR4/NF-κB signaling pathway. We expected that polyphenol phytochemicals targeting the TLR4/NF-κB signaling pathway might be an effective approach to treat IBD in future clinical research applications.
Inverse and Concordant Mucosal Pathway Gene Expressions in Inflamed and Non-Inflamed Ulcerative Colitis Patients: Potential Relevance to Aetiology and Pathogenesis
Authors: Söderman Jan , Berglind Linda , Almer Sven
Published: June 2022

Ulcerative colitis (UC) arises from a complex interplay between host and environmental factors, but with a largely unsolved pathophysiology. The pathophysiology was outlined by RNA-sequencing of mucosal biopsies from non-inflamed and inflamed colon of UC patients (14 and 17, respectively), and from 27 patients without intestinal inflammation. Genes differentially expressed (DE), or present in enriched gene sets, were investigated using statistical text analysis of functional protein information. Compared with controls, inflamed and non-inflamed UC mucosa displayed 9360 and 52 DE genes, respectively. Seventy-three non-pseudogenes were DE relative to both gender and inflammation. Mitochondrial processes were downregulated in inflamed and upregulated in non-inflamed UC mucosa, whereas angiogenesis and endoplasmic reticulum (ER) stress were upregulated in both tissue states. Immune responses were upregulated in inflamed mucosa, whereas the non-inflamed UC mucosa presented both up- and downregulated gene sets. DE and enriched genes overlapped with genes present in inflammatory bowel disease genome-wide associated loci (p = 1.43 × 10−18), especially regarding immune responses, respiratory chain, angiogenesis, ER stress, and steroid hormone metabolism. Apart from confirming established pathophysiological mechanisms of immune cells, our study provides evidence for involvement of less described pathways (e.g., respiratory chain, ER stress, fatty-acid oxidation, steroid hormone metabolism and angiogenesis).
Hsp90 Activity Is Necessary for the Maturation of Rabies Virus Polymerase
Authors: Dalidowska Iga , Orlowska Anna , Smreczak Marcin , Bieganowski Pawel
Published: June 2022

Mononegavirales is an order of viruses with a genome in the form of a non-segmented negative-strand RNA that encodes several proteins. The functional polymerase complex of these viruses is composed of two proteins: a large protein (L) and a phosphoprotein (P). The replication of viruses from this order depends on Hsp90 chaperone activity. Previous studies have demonstrated that Hsp90 inhibition results in the degradation of mononegaviruses L protein, with exception of the rabies virus, for which the degradation of P protein was observed. Here, we demonstrated that Hsp90 inhibition does not affect the expression of rabies L and P proteins, but it inhibits binding of the P protein and L protein into functional viral polymerase. Rabies and the vesicular stomatitis virus, but not the measles virus, L proteins can be expressed independently of the presence of a P protein and in the presence of an Hsp90 inhibitor. Our results suggest that the interaction of L proteins with P proteins and Hsp90 in the process of polymerase maturation may be a process specific to particular viruses.
Biodiversity and Distribution of Reticulitermes in the Southeastern USA
Authors: Johnson Allison , Forschler Brian T.
Published: June 2022

Reticulitermes subterranean termites are widely distributed ecosystem engineers and structural pests, yet describing their species distribution worldwide or regionally has been hindered by taxonomic uncertainties. Morphological plasticity confounds the use of taxonomic keys, while recent species descriptions and molecular techniques lacking taxonomic support have caused a muddle in interpreting the literature on Reticulitermes species distributions. We employed an integrative taxonomic approach combining behavioral, morphological, and molecular techniques to identify 4371 Reticulitermes samples to species. Five Reticulitermes species were collected from wood-on-ground at 1570 sites covering 153,900 km2 in the state of Georgia, USA. Three species were collected throughout Georgia, with R. flavipes identified from every one of the 159 counties. R. nelsonae was the second most frequently collected species, found in 128 counties, with R. virginicus third with 122. Two species had distributions confined to the northern part of the state. R. malletei was collected from 73 counties, while the least collected species, R. hageni, was found in 16. Results show that the most recently described species (R. nelsonae, 2012) is widely distributed and the second-most frequently encountered termite, representing 23% of all samples. The invasive species R. flavipes represented half of all the samples collected, while R. hageni, the least at less than 1%. A search of GenBank identified a number of accessions mismatched to a species designation resulting in the literature under-reporting the biodiversity of the genus. We, therefore, outline a path to standardize methods for species identification using an integrated taxonomic approach with appropriate barcodes for consistent identification across research teams worldwide. The data also illuminate new opportunities to examine questions related to the ecology, evolution, dispersal, and resource partitioning behaviors of these sympatric species across distinct geographical regions.
Single-Breath Counting Test to Start Non-Invasive Respiratory Support in COVID-19 Patients: Early Detection and the Eternal Dilemma. Comment on Longhitano et al. Single-Breath Counting Test Predicts Non-Invasive Respiratory Support Requirements in Patients with COVID-19 Pneumonia. J. Clin. Med. 2022, 11, 179
Authors: Oldani Stefano , Bensai Serena , Esquinas Antonio M.
Published: June 2022

We have read this study, in which the predictive role of a single-breath counting test (SBCT) to foresee the need of non-invasive respiratory strategies (NIRS) in patients with COVID-19 has been explored, with great interest [...]
The Use of Wood Pellets in the Production of High Quality Biocarbon Materials
Authors: Saletnik Bogdan , Saletnik Aneta , Zaguła Grzegorz , Bajcar Marcin , Puchalski Czesław
Published: June 2022

Biomass is one of the most important sources of renewable energy. One of the most widely used biomass biofuels is wood pellets. It is an economical, homogeneous and easy-to-use raw material. Biomass is used to generate low-emission energy utilizing the pyrolysis process. Pyrolysis allows for higher energy efficiency with the use of commonly available substrates. This thesis presents the results of research on the possibility of using the pyrolysis process to produce high-energy biocarbons from wood pellets. Data on basic energy parameters and explosivity of biocarbon dust were compiled as criteria for the attractiveness of the solution in terms of energy utility. The research used pellets made of oak, coniferous, and mixed sawdust, which were subjected to a pyrolysis process with varying temperature and time parameters. Carbon, ash, nitrogen, hydrogen, volatile substances, heavy metals, durability and calorific value of the tested materials were carried out. The highest increase in calorific value was determined to be 63% for biocarbons obtained at 500 ℃ and a time of 15 min, compared with the control sample. The highest calorific value among all analyzed materials was obtained from coniferous pellet biocarbon at 31.49 MJ kg−1. Parameters such as maximum explosion pressure, Pmax, maximum pressure increase over time, (dp/dt)max, and explosion rates, Kst max, were also analyzed. It was noted that biomass pyrolysis, which was previously pelletized, improved the energy parameters of the fuel and did not increase the risk class of dust explosion. The lowest and highest recorded values of Kst max for the analyzed materials were 76.53 and 94.75 bar s−1, respectively. The study concluded that the process used for processing solid biofuels did not affect the increase in the danger of dust explosion. The results presented in this article form the basis for further research to obtain detailed knowledge of the safety principles of production, storage, transport and use of these new fuels.
Titanium(IV) Oxo-Complex with Acetylsalicylic Acid Ligand and Its Polymer Composites: Synthesis, Structure, Spectroscopic Characterization, and Photocatalytic Activity
Authors: Śmigiel Julia , Muzioł Tadeusz , Piszczek Piotr , Radtke Aleksandra
Published: June 2022

The titanium oxo complexes are widely studied, due to their potential applications in photocatalytic processes, environmental protection, and also in the biomedical field. The presented results concern the oxo complex synthesized in the reaction of titanium(IV) isobutoxide and acetylsalicylic acid (Hasp), in a 4:1 molar ratio. The structure of isolated crystals was solved using the single-crystal X-ray diffraction method. The analysis of these data proves that [Ti4O2(OiBu)10(asp)2]·H2O (1) complex is formed. Moreover, the molecular structure of (1) was characterized using vibrational spectroscopic techniques (IR and Raman), 13C NMR, and UV–Vis diffuse reflectance spectroscopy (UV–Vis DRS). The photocatalytic activity of the synthesized complex was determined with the use of composite foils produced by the dispersion of (1) micrograins, as the inorganic blocks, in a polycaprolactone (PCL) matrix (PCL + (1)). The introduction of (1) micrograins to the PCL matrix caused the absorption maximum shift up to 425–450 nm. The studied PCL + (1) composite samples reveal good activity toward photodecolorization of methylene blue after visible light irradiation.
Superhydrophilic Modification of Polycarbonate Substrate Surface by Organic Plasma Polymerization Film
Authors: Lu Kuan-Wei , Lin Yu-Tian , Wei Hung-Sen , Kuo Chien-Cheng
Published: June 2022

Superhydrophilicity performs well in anti-fog and self-cleaning applications. In this study, polycarbonate substrate was used as the modification object because of the low surface energy characteristics of plastics. Procedures that employ plasma bombardment, such as etching and high surface free energy coating, are applied to improve the hydrophilicity. An organic amino silane that contains terminal amine group is introduced as the monomer to perform plasma polymerization to ensure that hydrophilic radicals can be efficiently deposited on substrates. Different levels of hydrophilicity can be reached by modulating the parameters of plasma bombardment and polymerization, such as plasma current, voltage of the ion source, and bombardment time. The surface of a substrate that is subjected to plasma bombarding at 150 V, 4 A for 5 min remained superhydrophilic for 17 days. After 40 min of Ar/O2 plasma bombardment, which resulted in a substrate surface roughness of 51.6 nm, the plasma polymerization of organic amino silane was performed by tuning the anode voltage and operating time of the ion source, and a water contact angle < 10° and durability up to 34 days can be obtained.
Correction Method for the Bending Characteristics of Aero Ball Joints under High Temperature and High Pressure
Authors: Yuan Jie , Yang Kaijie , Shi Hong , Yang Boxiao , Wang Jiangong , Jiang Yanlong
Published: June 2022

The aero ball joint is pivotal in aircraft duct systems due to its favorable properties, including displacement compensation and flexibility. In the stress assessment of air ducts, ball joints are usually simplified by using “Joints” connections to reduce the convergence problems caused by non-linearity, which requires a high degree of accuracy in the characteristic parameters of the ball joint. Accordingly, this paper builds a high temperature and pressure fatigue test platform to investigate the bending characteristics of the ball joint at different temperatures and pressures and points out the limitations of the current method. Then, a method combining finite element analysis (FEA) and the BP neural network is proposed to obtain the characteristic parameters of the ball joint. The results showed that the bending process of the ball joint tended to have two typically different stiffness properties, which were high rigidity and low rigidity. The bending characteristics were strongly influenced by pressure, but less influenced by temperature. The existing test platform increased the force reaction at the contact areas of the ball joint, resulting in errors in the measurement of characteristic parameters. The BP neural network prediction method could effectively alter the ball joint properties and reduce errors.
The Effect of (Mg, Zn)12Ce Phase Content on the Microstructure and the Mechanical Properties of Mg–Zn–Ce–Zr Alloy
Authors: Li Yuguang , Guo Feng , Cai Huisheng , Wang Yiwei , Liu Liang
Published: June 2022

The quantitative study of rare earth compounds is important for the improvement of existing magnesium alloy systems and the design of new magnesium alloys. In this paper, the effective separation of matrix and compound in Mg–Zn–Ce–Zr alloy was achieved by a low-temperature chemical phase separation technique. The mass fraction of the (Mg, Zn)12Ce compound was determined and the effect of the (Mg, Zn)12Ce phase content on the heat deformation organization and properties was investigated. The results show that the Mg–Zn–Ce compound in both the as-cast and the homogeneous alloys is (Mg, Zn)12Ce. (Mg, Zn)12Ce phase formation depends on the content and the ratio of Zn and Ce elements in the initial residual melt of the eutectic reaction. The Zn/Ce mass ratios below 2.5 give the highest compound contents for different Zn contents, 5.262 wt.% and 7.040 wt.%, respectively. The increase in the amount of the (Mg, Zn)12Ce phase can significantly reduce the critical conditions for dynamic recrystallization formation. Both the critical strain and the stress decrease with increasing rare earth content. The reduction of the critical conditions and the particle-promoted nucleation mechanism work together to increase the amount of dynamic recrystallization. In addition, it was found that alloys with 6 wt.% Zn elements tend to undergo a dynamic recrystallization softening mechanism, while alloys with 3 wt.% Zn elements tend to undergo a dynamic reversion softening mechanism.