bluuebunny/crossref_metadata_2025 huggingface dataset:
(Ab)use of Health Claims in Websites: The Case of Italian Bottled Waters
Authors: Lorenzoni Giulia , Minto Clara , Temporin Matteo , Fuscà Elisa , Bolzon Anna , Piras Gianluca , Iliceto Sabino , Silano Marco , Gregori Dario
Published: August 2019

The massive use of web marketing makes the monitoring of nutrition and health claims used in advertising campaigns much more difficult. The present study aimed at reviewing the website content for bottled waters produced in Italy to assess (i) if nutrition and health claims are reported, (ii) what types of nutrition and health claims are reported most frequently, and (iii) if the nutrition and health claims could be considered appropriate according to the current regulation in the field. A review of the website content of the 253 bottled waters produced in Italy and reported in the annual report of Bevitalia 2016–2017 was conducted. For each brand, indications related to the preventive, curative or therapeutic properties of the water reported were examined. Bottled waters that included potentially misleading information apparently not consistent with the European Directive on the exploitation and marketing of natural mineral waters were identified. Forty bottled waters with uncertain website content were identified. The information reported in the websites referred most often to beneficial effects for urinary tract and cardiovascular systems. Present results highlight, using the bottled water case study, that website content sometimes happens to deliver misleading information to consumers, also thanks to uncertain regulation in this sensitive field.
Higher Levels of Early Childhood Caries (ECC) Is Associated with Developing Psychomotor Deficiency: The Cross- Sectional Bi-Township Analysis for The New Hypothesis
Authors: Liang Chen-Yi , Liu Yen-Chun G. , Shieh Tien-Yu , Tseng Yi-Chun , Teng Andy Yen-Tung
Published: August 2019

The aim of this study was to reassess and confirm the relationship between early childhood caries (ECC) and manifestations of psychomotor deficiency in 4–6-yr-old kindergarteners, which has remained elusive to date. A cross-sectional study with bi-township analysis was designed whereby 353 kindergarteners, aged 4–6 whose caries were greater (dmft (decayed, missing and filled teeth, dmft index) = 5.25) than that of the national average, located in a rural township of central Taiwan were recruited using simple random-selection. Besides the personal, demographic, and dietary information, the measurements for caries and the amended comprehensive scales (CCDI) of children’s psychomotor development were used to address their relationship. One-way ANOVA vs. multiple linear regression were employed to compare the differences of variables between age, gender, BMI (Body Mass Index), and dmft scores vs. relationships among all variables, respectively. The results confirmed that there was a positive relationship between severe ECC (dmft > 3~8) and psychomotor deficiency (i.e., expressive language and comprehension-concept scales, etc.) amongst the kindergarteners analyzed. Our cross-sectional bi-township analysis has confirmed that there is indeed an association between severe ECC and psychomotor deficiency in kindergarteners, and we suggest that this may arise through critical stages of growth, not only via personal language communications, but psycho-social engagements as well. Therefore, a new hypothesis is proposed.
Healthcare Professionals’ Perceptions and Concerns towards Domestic Violence during Pregnancy in Southern Italy
Authors: Procentese Fortuna , Di Napoli Immacolata , Tuccillo Filomena , Chiurazzi Alessandra , Arcidiacono Caterina
Published: August 2019

Background: Literature on pregnancy highlighted a large number of women abused by their partners, especially among low-income teenagers attending hospital for pregnancy check-ups. Pregnancy represents a key moment for diagnosing domestic violence. Method: This study explores health professionals’ perceptions and concerns about domestic violence against women in services dealing with pregnant women. The twenty-four interviewees were from an Obstetrical-Gynecological walk-in Clinic in the south of Italy. The textual data has been complementarily analyzed by means of two different procedures: Symbolic-structural semiotic analysis and Thematic content analysis. Results: What emerges is that the interviewees of the clinic do not regard the issue of domestic violence as a matter of direct interest for the health service. The clinic is seen as a place for urgent contact, but one where there is not enough time to dedicate to this kind of patient, nor an adequate space to care for and listen to them. Obstetricians and health personnel expressed a negative attitude when it comes to including questions regarding violence and abuse in pre-natal reports. Training for health and social professionals and the empowering of institutional support and networking practices are needed to increase awareness of the phenomenon among the gynecological personnel.
Usability Testing of a Mobile Health Intervention to Address Acute Care Needs after Sexual Assault
Authors: Gilmore Amanda K. , Davidson Tatiana M. , Leone Ruschelle M. , Wray Lauren B. , Oesterle Daniel W. , Hahn Christine K. , Flanagan Julianne C. , Gill-Hopple Kathleen , Acierno Ron
Published: August 2019

Sexual assault is associated with a range of poor mental health outcomes. To enhance access to care by this population, technology-based mental health interventions have been implemented in the emergency room; however, more accessible and easily disseminated interventions are needed. The aim of the present study was to test the usability of a mobile health intervention targeting alcohol and drug misuse, suicide prevention, posttraumatic stress symptoms, coping skills, and referral to formal assistance for individuals who have experienced sexual assault. Feedback on the usability of the intervention was collected from individuals who received a sexual assault medical forensic examination (n = 13), and feedback on the usability and likelihood of recommending the application was collected from community providers (n = 25). Thematic analysis was used to describe qualitative data. Content themes related to aesthetics, usability, barriers to resources, and likes/dislikes about the intervention arose from interviews following the intervention. Participants found the intervention to be user friendly and endorsed more likes than dislikes. Providers rated the intervention as being helpful and would recommend it to survivors of sexual assault. Findings suggest that the intervention is usable and fit for future effectiveness testing, filling an important gap in treatment for individuals who experience sexual assault.
Examination of Human Health Impacts Due to Adverse Climate Events Through the Use of Vulnerability Mapping: A Scoping Review
Authors: Schmeltz Michael T. , Marcotullio Peter J.
Published: August 2019

Government officials, health professionals, and other decision makers are tasked with characterizing vulnerability and understanding how populations experience risks associated with exposure to climate-related hazards. Spatial analyses of vulnerable locations have given rise to climate change vulnerability mapping. While not a new concept, the spatial analyses of specific health outcomes remain limited. This review explores different methodologies and data that are used to assess vulnerability and map population health impacts to climate hazards. The review retrieved scholarly articles and governmental reports concerning vulnerability mapping of human health to the impacts of climate change in the United States, published in the last decade. After review, 37 studies were selected for inclusion. Climate-related exposures were distributed across four main categories, including: high ambient temperatures; flood hazards; vector-borne diseases; and wildfires. A number of different methodologies and measures were used to assess health vulnerability to climate-related hazards, including heat vulnerability indices and regression analyses. Vulnerability maps should exemplify how variables measuring the sensitivity and adaptive capacity of different populations help to determine the potential for climate-related hazards to have an effect on human health. Recommendations address methodologies, data gaps, and communication to assist researchers and stakeholders in directing adaptations to their most efficient and effective use.
Understanding Differences in Cancer Survival between Populations: A New Approach and Application to Breast Cancer Survival Differentials between Danish Regions
Authors: Bergeron-Boucher Marie-Pier , Oeppen Jim , Holm Niels Vilstrup , Nielsen Hanne Melgaard , Lindahl-Jacobsen Rune , Wensink Maarten Jan
Published: August 2019

Large variations in cancer survival have been recorded between populations, e.g., between countries or between regions in a country. To understand the determinants of cancer survival differentials between populations, researchers have often applied regression analysis. We here propose the use of a non-parametric decomposition method to quantify the exact contribution of specific components to the absolute difference in cancer survival between two populations. Survival differences are here decomposed into the contributions of differences in stage at diagnosis, population age structure, and stage-and-age-specific survival. We demonstrate the method with the example of differences in one-year and five-year breast cancer survival between Denmark’s five regions. Differences in stage at diagnosis explained 45% and 27%, respectively, of the one- and five-year survival differences between Zealand and Central Denmark for patients diagnosed between 2008 and 2010. We find that the introduced decomposition method provides a powerful complementary analysis and has several advantages compared with regression models: No structural or distributional assumptions are required; aggregated data can be used; and the use of absolute differences allows quantification of the survival that could be gained by improving, for example, stage at diagnosis relative to a reference population, thus feeding directly into health policy evaluation.
International Perspectives on the Implications of Cannabis Legalization: A Systematic Review & Thematic Analysis
Authors: Bahji Anees , Stephenson Callum
Published: August 2019

The legality, recreational and medical use of cannabis varies widely by country and region but remains largely prohibited internationally. In October 2018, Canada legalized the recreational use of cannabis—a move many viewed as controversial. Proponents of legalization have emphasized the potential to eradicate the marijuana black market, improve quality and safety control, increase tax revenues, improve the availability of medical cannabis, and lower gang-related drug violence. Conversely, opponents of legalization have stressed concerns about cannabis’ addictive potential, second-hand cannabis exposure, potential exacerbation of underlying and established mental illnesses, as well as alterations in perception that affect safety, particularly driving. This systematic review synthesizes recent international literature on the clinical and public health implications of cannabis legalization.
Spatio-Temporal Analysis of Drought Variability Using CWSI in the Koshi River Basin (KRB)
Authors: Wu Han , Xiong Donghong , Liu Bintao , Zhang Su , Yuan Yong , Fang Yiping , Chidi Chhabi Lal , Dahal Nirmal Mani
Published: August 2019

Drought is one of the most frequent meteorological disasters, and has exerted significant impacts on the livelihoods and economy of the Koshi River Basin (KRB). In this study, we assessed drought patterns using the Crop Water Shortage Index (CWSI) based on the MOD16 product for the period between 2000 and 2014. The results revealed that the CWSI based on the MOD16 product can be act as an indicator to monitor the characteristics of the drought. Significant spatial heterogeneity of drought was observed in the basin, with higher CWSI values downstream and upstream than in the midstream. The midstream of the KRB was dominated by light drought, moderate drought occurred in the upstream, and the downstream was characterized by severe drought. The monthly CWSI during one year in KRB showed the higher CWSI between March to May (pre-monsoon) and October to December (post-monsoon) rather than June to September (monsoon), and the highest was observed in the month of April, suggesting that precipitation plays the most important role in the mitigation of CWSI. Additionally, the downstream and midstream showed a higher variation of drought compared to the upstream in the basin. This research indicates that the downstream suffered severe drought due to seasonal water shortages, especially during the pre-monsoon, and water-related infrastructure should be implemented to mitigate losses caused by drought.
Exploring Psychophysiological Restoration and Individual Preference in the Different Environments Based on Virtual Reality
Authors: Gao Tian , Zhang Tian , Zhu Ling , Gao Yanan , Qiu Ling
Published: August 2019

Accumulated evidence claims that urban green spaces (UGS) have a positive impact on the physical and mental health of humans. However, little information is available to clearly reveal what the most important driving factors are for human psychophysiological restoration. In order to unveil this uncertainty, this study employed virtual reality (VR) technology to investigate the physiological (electroencephalogram, EEG), and psychological (attention, positive mood, negative mood) responses and individual preferences for different urban environments. Participants (120) were recruited and randomly assigned to experience six different types of environments varying in land use and vegetation structures, which were: Grey space, blue space, open green space, partly open green space, partly closed green space, and closed green space. The results showed that the experience of the six environmental types through VR devices had positive restorative effects on the individuals’ attentional fatigue and negative mood; however, all the participants obtained the highest levels of physiological stress restoration when asked to close their eyes for relaxation. The physiological measurements of the EEG showed no significant differences among the selected types of environments. Meanwhile, the results of the psychological measures suggested that only negative mood showed significant differences of change among the six types of environments, and while the partly open green space had the most positive effect on negative mood, the closed green space had the worst. The blue space and partly closed green space received higher recreational preference ratings than the other four environments, while the closed green space received the lowest recreational preference rating. Moreover, the findings showed that there was a strong positive correlation between people’s preferences and the improvement of their positive mood. This indicated that as the popularity of a natural environment increased, so did the benefits of human health and well-being. In addition, this study shows that VR technology may be utilized as a possible surrogate measure to real scenes in evaluating human physiological and psychological restoration in the future. The present findings can provide the theoretical basis and practical guidance for future optimal planning of urban restorative environments.
Assessment of Agricultural Carbon Emissions and Their Spatiotemporal Changes in China, 1997–2016
Authors: Huang Xiuquan , Xu Xiaocang , Wang Qingqing , Zhang Lu , Gao Xin , Chen Linhong
Published: August 2019

Despite achieving remarkable development, China’s agricultural economy has been under severe environmental pressure. Based on previous studies, the present study further considers the sources of agricultural carbon emissions in depth, estimates China’s agricultural carbon emissions from 1997 to 2016, and analyzes the agricultural pollution faced by China and its provinces. The study estimates the amount and intensity of agricultural carbon emissions in China from five carbon sources—agricultural materials, rice planting, soil N2O, livestock and poultry farming, and straw burning—and analyzes their spatial and temporal characteristics. The following results were obtained: (1) between 1997 and 2016, the amount of agricultural carbon emissions in China generally increased, while the intensity of agricultural carbon emissions decreased; (2) in the same period, the amount of carbon emissions from each category of carbon source generally increased, with the exception of rice planting; however, the amount of emissions fluctuated; (3) the amount and intensity of carbon emissions varied greatly among provinces; (4) the emissions from different categories of carbon source showed different concentration trends and agglomeration forms; (5) China’s agricultural carbon emissions showed obvious spatial correlation, which overall was high–high agglomeration; however, its carbon emissions gradually weakened, and the spatial agglomeration of agricultural carbon emissions in each province changed between 1997 and 2016.
Translation and Validation of the Volunteer Functions Inventory (VFI) among the General Dutch Older Population
Authors: Niebuur Jacobien , Liefbroer Aart C. , Steverink Nardi , Smidt Nynke
Published: August 2019

Improvement of volunteering rates in the Netherlands is important because increased productivity among older adults would contribute to societal sustainability in the light of population aging. Therefore, a better understanding of volunteer motivations of Dutch older adults is needed. The Volunteer Functions Inventory (VFI) for assessing volunteer motivations has good psychometric properties and is adapted to several languages, but no validated Dutch translation yet exists. The aim of the current study is to validate the VFI for use in the Dutch older population (60 years and over). The Dutch-translated VFI (6 scales, 30 items) is included in the Lifelines ‘Daily Activities and Leisure Activities add-on Study’, which was distributed among participants aged 60 to 80. Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) are performed to assess the validity of the translated VFI. Internal consistency is assessed by computing Cronbach’s α’s. Results of the EFA (N = 4208) point towards a six-factor solution with a nearly perfectly clean structure. Deletion of three problematic items results in a clean factor structure. CFA results indicate moderate model fit (RMSEA = 0.06, CFI = 0.90, TLI = 0.89). Cronbach’s α’s (0.78 to 0.85) indicate good internal consistency. Goodness-of-fit indices are sufficient and comparable to those obtained in the validation of the original VFI. The current study provides support for use of the Dutch-translation of the VFI (6 scales, 27 items) to assess volunteer motivations among Dutch volunteers aged 60 years and over.
Distributed Processing of Location-Based Aggregate Queries Using MapReduce
Authors: Huang Yuan-Ko
Published: August 2019

The location-based aggregate queries, consisting of the shortest average distance query (SAvgDQ), the shortest minimal distance query (SMinDQ), the shortest maximal distance query (SMaxDQ), and the shortest sum distance query (SSumDQ) are new types of location-based queries. Such queries can be used to provide the user with useful object information by considering both the spatial closeness of objects to the query object and the neighboring relationship between objects. Due to a large amount of location-based aggregate queries that need to be evaluated concurrently, the centralized processing system would suffer a heavy query load, leading eventually to poor performance. As a result, in this paper, we focus on developing the distributed processing technique to answer multiple location-based aggregate queries, based on the MapReduce platform. We first design a grid structure to manage information of objects by taking into account the storage balance, and then develop a distributed processing algorithm, namely the MapReduce-based aggregate query algorithm (MRAggQ algorithm), to efficiently process the location-based aggregate queries in a distributed manner. Extensive experiments using synthetic and real datasets are conducted to demonstrate the scalability and the efficiency of the proposed processing algorithm.
The Acute Effects of Oral Administration of Phytic Acid-Chitosan-Magnetic Iron Oxide Nanoparticles in Mice
Authors: Mohd Tamsir Norain , Mohd Esa Norhaizan , Shafie Nurul Husna , Hussein Mohd Zobir , Hamzah Hazilawati , Abdullah Maizaton Atmadini
Published: August 2019

A nanocomposite, phytic acid-chitosan-magnetic iron oxide nanoparticles (IP6-CS-MNPs) has been used to treat colon cancer in vitro, previously. However, its potential toxicity in vivo has yet to be elucidated. Hence, the present study aimed to evaluate the acute effects of oral administration of IP6-CS-MNPs in mice. In this study, 1000 and 2000 mg/kg body weight (b.w) of IP6-CS-MNPs were orally administered to two different groups of BALB/c mice, once. Additionally, the mice in the control group were given only deionized water. After 14 days of post-IP6-CS-MNPs administration, in a similar way to the untreated mice, the treated mice showed no sign of mortality and abnormalities. However, the serum urea level of mice receiving 2000 mg/kg b.w of IP6-CS-MNPs was significantly higher than the control group (p < 0.05). The mice that received 1000 mg/kg IP6-CS-MNPs showed a significantly higher level of serum alkaline phosphatase (ALP) compared to the control group. However, there were no significant histopathological changes seen in the liver and kidneys of treated mice compared to the untreated group.
3dRNA v2.0: An Updated Web Server for RNA 3D Structure Prediction
Authors: Wang Jun , Wang Jian , Huang Yanzhao , Xiao Yi
Published: August 2019

3D structures of RNAs are the basis for understanding their biological functions. However, experimentally solved RNA 3D structures are very limited in comparison with known RNA sequences up to now. Therefore, many computational methods have been proposed to solve this problem, including our 3dRNA. In recent years, 3dRNA has been greatly improved by adding several important features, including structure sampling, structure ranking and structure optimization under residue-residue restraints. Particularly, the optimization procedure with restraints enables 3dRNA to treat pseudoknots in a new way. These new features of 3dRNA can greatly promote its performance and have been integrated into the 3dRNA v2.0 web server. Here we introduce these new features in the 3dRNA v2.0 web server for the users.
Modulation of Cardiovascular Function in Primary Hypertension in Rat by SKA-31, an Activator of KCa2.x and KCa3.1 Channels
Authors: Kloza Monika , Baranowska-Kuczko Marta , Toczek Marek , Kusaczuk Magdalena , Sadowska Olga , Kasacka Irena , Kozłowska Hanna
Published: August 2019

The aim of this study was to investigate the hemodynamic effects of SKA-31, an activator of the small (KCa2.x) and intermediate (KCa3.1) conductance calcium-activated potassium channels, and to evaluate its influence on endothelium-derived hyperpolarization (EDH)-KCa2.3/KCa3.1 type relaxation in isolated endothelium-intact small mesenteric arteries (sMAs) from spontaneously hypertensive rats (SHRs). Functional in vivo and in vitro experiments were performed on SHRs or their normotensive controls, Wistar-Kyoto rats (WKY). SKA-31 (1, 3 and 10 mg/kg) caused a brief decrease in blood pressure and bradycardia in both SHR and WKY rats. In phenylephrine-pre-constricted sMAs of SHRs, SKA-31 (0.01–10 µM)-mediated relaxation was reduced and SKA-31 potentiated acetylcholine-evoked endothelium-dependent relaxation. Endothelium denudation and inhibition of nitric oxide synthase (eNOS) and cyclooxygenase (COX) by the respective inhibitors l-NAME or indomethacin, attenuated SKA-31-mediated vasorelaxation. The inhibition of KCa3.1, KCa2.3, KIR and Na+/K+-ATPase by TRAM-34, UCL1684, Ba2+ and ouabain, respectively, reduced the potency and efficacy of the EDH-response evoked by SKA-31. The mRNA expression of eNOS, prostacyclin synthase, KCa2.3, KCa3.1 and KIR were decreased, while Na+/K+-ATPase expression was increased. Collectively, SKA-31 promoted hypotension and vasodilatation, potentiated agonist-stimulated vasodilation, and maintained KCa2.3/KCa3.1-EDH-response in sMAs of SHR with downstream signaling that involved KIR and Na+/K+-ATPase channels. In view of the importance of the dysfunction of endothelium-mediated vasodilatation in the mechanism of hypertension, application of activators of KCa2.3/KCa3.1 channels such as SKA-31 seem to be a promising avenue in pharmacotherapy of hypertension.
Probiotics for Parkinson’s Disease
Authors: Gazerani Parisa
Published: August 2019

Parkinson’s disease (PD) is a complex neurological disorder classically characterized by impairments in motor system function associated with loss of dopaminergic neurons in the substantia nigra. After almost 200 years since the first description of PD by James Parkinson, unraveling the complexity of PD continues to evolve. It is now recognized that an interplay between genetic and environmental factors influences a diverse range of cellular processes, reflecting on other clinical features including non-motor symptoms. This has consequently highlighted the extensive value of early clinical diagnosis to reduce difficulties of later stage management of PD. Advancement in understanding of PD has made remarkable progress in introducing new tools and strategies such as stem cell therapy and deep brain stimulation. A link between alterations in gut microbiota and PD has also opened a new line. Evidence exists of a bidirectional pathway between the gastrointestinal tract and the central nervous system. Probiotics, prebiotics and synbiotics are being examined that might influence gut-brain axis by altering gut microbiota composition, enteric nervous system, and CNS. This review provides status on use of probiotics for PD. Limitations and future directions will also be addressed to promote further research considering use of probiotics for PD.
The Complex Phosphorylation Patterns That Regulate the Activity of Hsp70 and Its Cochaperones
Authors: Velasco Lorea , Dublang Leire , Moro Fernando , Muga Arturo
Published: August 2019

Proteins must fold into their native structure and maintain it during their lifespan to display the desired activity. To ensure proper folding and stability, and avoid generation of misfolded conformations that can be potentially cytotoxic, cells synthesize a wide variety of molecular chaperones that assist folding of other proteins and avoid their aggregation, which unfortunately is unavoidable under acute stress conditions. A protein machinery in metazoa, composed of representatives of the Hsp70, Hsp40, and Hsp110 chaperone families, can reactivate protein aggregates. We revised herein the phosphorylation sites found so far in members of these chaperone families and the functional consequences associated with some of them. We also discuss how phosphorylation might regulate the chaperone activity and the interaction of human Hsp70 with its accessory and client proteins. Finally, we present the information that would be necessary to decrypt the effect that post-translational modifications, and especially phosphorylation, could have on the biological activity of the Hsp70 system, known as the “chaperone code”.
Kainate Receptor-Mediated Depression of Glutamate Release Involves Protein Kinase A in the Cerebellum
Authors: Falcón-Moya Rafael , Losada-Ruiz Pilar , Rodríguez-Moreno Antonio
Published: August 2019

Kainate (KA) receptors (KAR) have important modulatory roles of synaptic transmission. In the cerebellum, the action mechanisms of KAR-mediated glutamatergic depression are unknown. We studied these mechanisms by recording evoked excitatory postsynaptic currents (eEPSCs) from cerebellar slices using the whole-cell configuration of the patch-clamp technique. We observed that 3 μM KA decreased the amplitude of eEPSCs and increased the number of failures at the synapses established between parallel fibers (PF) and Purkinje neurons, and the effect was antagonized by NBQX under the condition where AMPA receptors were previously blocked. The inhibition of protein kinase A (PKA) suppressed the effect of KAR activation on eEPSC, and effect was not prevented by protein kinase C inhibitors. Furthermore, in the presence of Pertussis toxin, the depression of glutamate release mediated by KAR activation was prevented, invoking the participation of a Gi/o protein in this modulation. Finally, the KAR-mediated depression of glutamate release was not prevented by blocking calcium-permeable KARs or by treatments that affect calcium release from intracellular stores. We conclude that KARs present at these synapses mediate an inhibition of glutamate release through a mechanism that involves the activation of G-protein and protein kinase A.
PtrARF2.1 Is Involved in Regulation of Leaf Development and Lignin Biosynthesis in Poplar Trees
Authors: Fu Yongyao , Win Papa , Zhang Huijuan , Li Chaofeng , Shen Yun , He Fu , Luo Keming
Published: August 2019

Auxin response factors (ARFs) are important regulators modulating the expression of auxin-responsive genes in various biological processes in plants. In the Populus genome, a total of 39 ARF members have been identified, but their detailed functions are still unclear. In this study, six poplar auxin response factor 2 (PtrARF2) members were isolated from P. trichocarpa. Expression pattern analysis showed that PtrARF2.1 is highly expressed in leaf tissues compared with other PtrARF2 genes and significantly repressed by exogenous auxin treatment. PtrARF2.1 is a nuclear-localized protein without transcriptional activation activity. Knockdown of PtrARF2.1 by RNA interference (RNAi) in poplars led to the dwarf plant, altered leaf shape, and reduced size of the leaf blade, while overexpression of PtrARF2.1 resulted in a slight reduction in plant height and the similar leaf phenotype in contrast to the wildtype. Furthermore, histological staining analysis revealed an ectopic deposition of lignin in leaf veins and petioles of PtrARF2.1-RNAi lines. RNA-Seq analysis showed that 74 differential expression genes (DEGs) belonging to 12 transcription factor families, such as NAM, ATAF and CUC (NAC), v-myb avian myeloblastosis viral oncogene homolog (MYB), ethylene response factors (ERF) and basic helix–loop–helix (bHLH), were identified in PtrARF2.1-RNAi leaves and other 24 DEGs were associated with the lignin biosynthetic pathway. Altogether, the data indicate that PtrARF2.1 plays an important role in regulating leaf development and influences the lignin biosynthesis in poplars.
The Clinical Link between Human Intestinal Microbiota and Systemic Cancer Therapy
Authors: Aarnoutse Romy , Ziemons Janine , Penders John , Rensen Sander S. , de Vos-Geelen Judith , Smidt Marjolein L.
Published: August 2019

Clinical interest in the human intestinal microbiota has increased considerably. However, an overview of clinical studies investigating the link between the human intestinal microbiota and systemic cancer therapy is lacking. This systematic review summarizes all clinical studies describing the association between baseline intestinal microbiota and systemic cancer therapy outcome as well as therapy-related changes in intestinal microbiota composition. A systematic literature search was performed and provided 23 articles. There were strong indications for a close association between the intestinal microbiota and outcome of immunotherapy. Furthermore, the development of chemotherapy-induced infectious complications seemed to be associated with the baseline microbiota profile. Both chemotherapy and immunotherapy induced drastic changes in gut microbiota composition with possible consequences for treatment efficacy. Evidence in the field of hormonal therapy was very limited. Large heterogeneity concerning study design, study population, and methods used for analysis limited comparability and generalization of results. For the future, longitudinal studies investigating the predictive ability of baseline intestinal microbiota concerning treatment outcome and complications as well as the potential use of microbiota-modulating strategies in cancer patients are required. More knowledge in this field is likely to be of clinical benefit since modulation of the microbiota might support cancer therapy in the future.
Formation of α-Farnesene in Tea (Camellia sinensis) Leaves Induced by Herbivore-Derived Wounding and Its Effect on Neighboring Tea Plants
Authors: Wang Xuewen , Zeng Lanting , Liao Yinyin , Li Jianlong , Tang Jinchi , Yang Ziyin
Published: August 2019

Herbivore-induced plant volatiles (HIPVs) play important ecological roles in defense against stresses. In contrast to model plants, reports on HIPV formation and function in crops are limited. Tea (Camellia sinensis) is an important crop in China. α-Farnesene is a common HIPV produced in tea plants in response to different herbivore attacks. In this study, a C. sinensis α-farnesene synthase (CsAFS) was isolated, cloned, sequenced, and functionally characterized. The CsAFS recombinant protein produced in Escherichia coli was able to transform farnesyl diphosphate (FPP) into α-farnesene and also convert geranyl diphosphate (GPP) to β-ocimene in vitro. Furthermore, transient expression analysis in Nicotiana benthamiana plants indicated that CsAFS was located in the cytoplasm and could convert FPP to α-farnesene in plants. Wounding, to simulate herbivore damage, activated jasmonic acid (JA) formation, which significantly enhanced the CsAFS expression level and α-farnesene content. This suggested that herbivore-derived wounding induced α-farnesene formation in tea leaves. Furthermore, the emitted α-farnesene might act as a signal to activate antibacterial-related factors in neighboring undamaged tea leaves. This research advances our understanding of the formation and signaling roles of common HIPVs in crops such as tea plants.
The Role of SATB1 in Tumour Progression and Metastasis
Authors: Glatzel-Plucińska Natalia , Piotrowska Aleksandra , Dzięgiel Piotr , Podhorska-Okołów Marzenna
Published: August 2019

Carcinogenesis is a long-drawn, multistep process, in which metastatic spread is an unequivocal hallmark of a poor prognosis. The progression and dissemination of epithelial cancers is commonly thought to rely on the epidermal-mesenchymal transition (EMT) process. During EMT, epithelial cells lose their junctions and apical-basal polarity, and they acquire a mesenchymal phenotype with its migratory and invasive capabilities. One of the proteins involved in cancer progression and EMT may be SATB1 (Special AT-Rich Binding Protein 1)—a chromatin organiser and a global transcriptional regulator. SATB1 organizes chromatin into spatial loops, providing a “docking site” necessary for the binding of further transcription factors and chromatin modifying enzymes. SATB1 has the ability to regulate whole sets of genes, even those located on distant chromosomes. SATB1 was found to be overexpressed in numerous malignancies, including lymphomas, breast, colorectal, prostate, liver, bladder and ovarian cancers. In the solid tumours, an elevated SATB1 level was observed to be associated with an aggressive phenotype, presence of lymph node, distant metastases, and a poor prognosis. In this review, we briefly describe the prognostic significance of SATB1 expression in most common human cancers, and analyse its impact on EMT and metastasis.
Glucobrassicin Metabolites Ameliorate the Development of Portal Hypertension and Cirrhosis in Bile Duct-Ligated Rats
Authors: Chang Ting , Ho Hsin-Ling , Hsu Shao-Jung , Chang Ching-Chih , Tsai Ming-Hung , Huo Teh-Ia , Huang Hui-Chun , Lee Fa-Yauh , Hou Ming-Chih , Lee Shou-Dong
Published: August 2019

Patients suffering from liver cirrhosis are often complicated with the formation of portosystemic collateral vessels, which is associated with the progression of a splanchnic hyperdynamic circulatory state. Alleviating pathological angiogenesis has thus been proposed to be a feasible treatment strategy. Indole-3-carbinol (C9H9NO, I3C) and 3,3′-diindolymethane (DIM), formed by the breakdown of glucosinolate glucobrassicin, are prevalent in cruciferous vegetables and have anti-angiogenesis properties. We aimed to evaluate their influences on portal hypertension, the severity of mesenteric angiogenesis, and portosystemic collaterals in cirrhosis. Sprague-Dawley rats with common bile duct ligation (CBDL)-induced liver cirrhosis or sham operation (surgical control) were randomly allocated to receive I3C (20 mg/kg/3 day), DIM (5 mg/kg/day) or vehicle for 28 days. The systemic and portal hemodynamics, severity of portosystemic shunting, mesenteric angiogenesis, and mesenteric proangiogenic factors protein expressions were evaluated. Compared to vehicle, both DIM and I3C significantly reduced portal pressure, ameliorated liver fibrosis, and down-regulated mesenteric protein expressions of vascular endothelial growth factor and phosphorylated Akt. DIM significantly down-regulated pErk, and I3C down-regulated NFκB, pIκBα protein expressions, and reduced portosystemic shunting degree. The cruciferous vegetable byproducts I3C and DIM not only exerted a portal hypotensive effect but also ameliorated abnormal angiogenesis and portosystemic collaterals in cirrhotic rats.
Use of the Secreted Proteome of Trametes versicolor for Controlling the Cereal Pathogen Fusarium langsethiae
Authors: Parroni Alessia , Bellabarba Agnese , Beccaccioli Marzia , Scarpari Marzia , Reverberi Massimo , Infantino Alessandro
Published: August 2019

Fusarium langsethiae is amongst the most recently discovered pathogens of small grains cereals. F. langsethiae is the main producer, in Europe, of T2 and HT-toxins in small grain cereals, albeit often asymptomatic; this makes its control challenging. The European Union (EU) is pushing hard on the use of biocontrol agents to minimize the use of fungicides and pesticides, which are detrimental to the environment and responsible for serious pollution of the soil and superficial water. In line with EU directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae. T. versicolor, a so-called medicinal mushroom which is applied as a co-adjuvant in oncology and other pathologies as a producer of biological response modifiers. In this study, the exo-proteome of T. versicolor proved highly efficient in inhibiting the growth of F. langsethiae and the biosynthesis of the T2 toxin. Results are promising for its future use as a sustainable product to control F. langsethiae infection in cereals under field conditions.
Identification of a Potential Founder Effect of a Novel PDZD7 Variant Involved in Moderate-to-Severe Sensorineural Hearing Loss in Koreans
Authors: Lee Sang-Yeon , Han Jin Hee , Kim Bong Jik , Oh Seung Ha , Lee Seungmin , Oh Doo-Yi , Choi Byung Yoon
Published: August 2019

PDZD7, a PDZ domain-containing scaffold protein, is critical for the organization of Usher syndrome type 2 (USH2) interactome. Recently, biallelic PDZD7 variants have been associated with autosomal-recessive, non-syndromic hearing loss (ARNSHL). Indeed, we identified novel, likely pathogenic PDZD7 variants based on the American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines from Korean families manifesting putative moderate-to-severe prelingual ARNSHL; these were c.490C>T (p.Arg164Trp), c.1669delC (p.Arg557Glyfs*13), and c.1526G>A (p.Gly509Glu), with p.Arg164Trp being a predominantly recurring variant. Given the recurring missense variant (p.Arg164Trp) from our cohort, we compared the genotyping data using six short tandem-repeat (STR) markers within or flanking PDZD7 between four probands carrying p.Arg164Trp and 81 normal-hearing controls. We observed an identical haplotype across three out of six STR genotyping markers exclusively shared by two unrelated hearing impaired probands but not by any of the 81 normal-hearing controls, suggesting a potential founder effect. However, STR genotyping, based on six STR markers, revealed various p.Arg164Trp-linked haplotypes shared by all of the affected subjects. In conclusion, PDZD7 can be an important causative gene for moderate to severe ARNSHL in Koreans. Moreover, at least some, if not all, p.Arg164Trp alleles in Koreans could exert a potential founder effect and arise from diverse haplotypes as a mutational hot spot.
FKRR-MVSF: A Fuzzy Kernel Ridge Regression Model for Identifying DNA-Binding Proteins by Multi-View Sequence Features via Chou’s Five-Step Rule
Authors: Zou Yi , Ding Yijie , Tang Jijun , Guo Fei , Peng Li
Published: August 2019

DNA-binding proteins play an important role in cell metabolism. In biological laboratories, the detection methods of DNA-binding proteins includes yeast one-hybrid methods, bacterial singles and X-ray crystallography methods and others, but these methods involve a lot of labor, material and time. In recent years, many computation-based approachs have been proposed to detect DNA-binding proteins. In this paper, a machine learning-based method, which is called the Fuzzy Kernel Ridge Regression model based on Multi-View Sequence Features (FKRR-MVSF), is proposed to identifying DNA-binding proteins. First of all, multi-view sequence features are extracted from protein sequences. Next, a Multiple Kernel Learning (MKL) algorithm is employed to combine multiple features. Finally, a Fuzzy Kernel Ridge Regression (FKRR) model is built to detect DNA-binding proteins. Compared with other methods, our model achieves good results. Our method obtains an accuracy of 83.26% and 81.72% on two benchmark datasets (PDB1075 and compared with PDB186), respectively.
Transcription Repressor Hes1 Contributes to Neuropathic Pain Development by Modifying CDK9/RNAPII-Dependent Spinal mGluR5 Transcription
Authors: Hsieh Ming-Chun , Peng Hsien-Yu , Ho Yu-Cheng , Lai Cheng-Yuan , Cheng Jen-Kun , Chen Gin-Den , Lin Tzer-Bin
Published: August 2019

Diverse transcriptional controls in the dorsal horn have been observed in pain hypersensitivity. However, the understanding of the exact causes and mechanisms of neuropathic pain development is still fragmentary. Here, the results demonstrated nerve injury decreased the expression of spinal hairy and enhancer of split 1 (Hes1), a transcriptional repressor, and enhanced metabotropic glutamate receptor subtype 5 (mGluR5) transcription/expression, which was accompanied with behavioral allodynia. Moreover, nerve injury decreased Hes1 levels and reciprocally increased cyclin dependent kinase-9 (CDK9) levels and recruited CDK9 to phosphorylate RNA polymerase II (RNAPII) in the promoter fragments of mGluR5, thereby enhancing mGluR5 transcription/expression in the dorsal horn. These effects were also induced by intrathecally administering naïve rats with Hes1 small interfering RNA (siRNA). Conversely, Hes1 overexpression using intrathecal lentiviral vectors in nerve injury rats produced reversal of pain behavior and reversed protein expressions, phosphorylation, and coupling to the promoter segments in the dorsal horn. Collectively, the results in this study indicated nerve injury diminishes spinal Hes1-dependent suppression of CDK9-dependent RNAPII phosphorylation on the mGluR5 promoter that possibly enhances mGluR5 transcription/expression for neuropathic pain development.
Enhanced Grid-Based Visual Analysis of Retinal Layer Thickness with Optical Coherence Tomography
Authors: Röhlig Martin , Prakasam Ruby Kala , Stüwe Jörg , Schmidt Christoph , Stachs Oliver , Schumann Heidrun
Published: August 2019

Optical coherence tomography enables high-resolution 3D imaging of retinal layers in the human eye. The thickness of the layers is commonly assessed to understand a variety of retinal and systemic disorders. Yet, the thickness data are complex and currently need to be considerably reduced prior to further processing and analysis. This leads to a loss of information on localized variations in thickness, which is important for early detection of certain retinal diseases. We propose an enhanced grid-based reduction and exploration of retinal thickness data. Alternative grids are computed, their representation quality is rated, and best fitting grids for given thickness data are suggested. Selected grids are then visualized, adapted, and compared at different levels of granularity. A visual analysis tool bundles all computational, visual, and interactive means in a flexible user interface. We demonstrate the utility of our tool in a complementary analysis procedure, which eases the evaluation of ophthalmic study data. Ophthalmologists successfully applied our solution to study localized variations in thickness of retinal layers in patients with diabetes mellitus.
Preparation and Characterization of Large Area Li-NASICON Electrolyte Thick Films
Authors: Jiménez Ricardo , Sobrados Isabel , Martínez-Chaparro Sandra , del Campo Angel , Calzada M. , Sanz Jesús , Tsai Shu , Lin Ming , Fung Kuan , Kazakevicius Edvardas , Kežionis Algimantas
Published: August 2019

The preparation of solid electrolyte ceramic membranes is the object of intense study for its fundamental parts in the development of all solid-state batteries and improved battery separators. In this work, the procurement of large area solid electrolyte ceramic thick film membranes of the Li-NASICON Li1.3Al0.3Ti1.7(PO4)3 (LATP) composition is attempted. Through the use of LATP powders from a sol–gel reaction, a slurry is formulated and tape casted. The green tapes are sintered using two sintering times. In both cases, ceramic thick films of a 5.5 × 5.5 cm2 area and ≈250 µm average thickness were obtained. The characterization indicated almost pure phase samples with a bi-modal microstructure composed of large and smaller grains, being larger for longer sintering time. The samples are porous and brittle, presenting very high “bulk” conductivity but lower total direct current (DC) one, as compared with the commercial Li-NASICON (OHARA) thick films with a similar area. The larger the grains, the poorer the total conductivity and the mechanical properties of the thick-films. The formation of poorly adhering grain boundaries as the grain size grows is responsible for the worsened properties. A better control of the microstructure is mandatory.
Subterranean Acoustic Activity Patterns of Vitacea polistiformis (Lepidoptera: Sesiidae) in Relation to Abiotic and Biotic Factors
Authors: Inyang Edidiong I. , Hix Raymond L. , Tsolova Violeta , Rohde Barukh B. , Dosunmu Omotola , Mankin Richard W.
Published: August 2019

Grape root borer (GRB), Vitacea polistiformis, is a root-feeding pest of grapevines in the US southeast that causes underground damage well before vines show visible symptoms. A 269-d study was conducted at 31 sites in a Florida vineyard to record short bursts of insect movement and feeding vibrations in grapevine root systems and provide information that can improve timing and targeting of GRB management efforts. Characteristic spectral and temporal patterns in the subterranean vibrations facilitated discrimination of GRB from background noise and non-targeted arthropods. Infestation likelihood of GRB at each site was estimated from previous studies relating infestation to burst rate. In all, 39% of recordings indicated low infestation likelihood. Sites with medium or high infestation likelihood were confined to a small region of the vineyard where a vine with larval feeding damage was confirmed. The restricted area suggests that the biological control or chemical treatments could be reduced elsewhere. Acoustic activity was significantly greater in fall and winter than in spring, and greater in evening than afternoon; fall evenings seemed best for GRB acoustic surveys. The GRB seasonal and circadian acoustic variation reflected phenological variation in grape root growth and nutrients and was not significantly correlated with temperature.