bluuebunny/crossref_metadata_2025 huggingface dataset:
S-Adenosylmethionine (SAMe) for Liver Health: A Systematic Review
Authors: Baden Kyrie , McClain Halley , Craig Eliya , Gibson Nathan , Draime Juanita , Chen Aleda
Published: October 2024

Background/Objectives: S-adenosylmethionine (SAMe) is a natural compound implicated in the treatment of liver dysfunction. In this systematic review, our objective was to determine the efficacy, safety, and optimal dose of SAMe in liver diseases. Methods: Using the PRISMA methodology, we searched PubMed, CINAHL, and Web of Science using key MeSH search terms. For title/abstract screening, full-text review, and data extraction, two independent researchers reviewed articles, and a third researcher resolved conflicts. Data extraction also included a quality assessment of included articles. Results: Of the 1881 non-duplicated studies, 15 articles focusing on SAMe use in the liver were included. All included studies (n = 15) scored a 4 or 5 out of 5 points on the quality assessment, which indicated high study quality. Overall, SAMe was effective in improving liver-related parameters with few adverse events, which were primarily mild, transient gastrointestinal complaints. Conclusions: The most common doses were SAMe 1000 mg or 1200 mg per day with or without another treatment or natural supplement. Future studies are needed to assess long-term efficacy and safety data of SAMe and the optimal route of administration in liver diseases.
Portion Size Norms of Discretionary Foods and Eating Settings: A Repeated Cross-Sectional Study
Authors: Liu Qingzhou , Allman-Farinelli Margaret , Rangan Anna
Published: October 2024

Background: The increase in serving sizes of energy-dense nutrient-poor discretionary foods over time, with attractively presented large servings and package sizes, has led to portion distortion and a new ‘normal’ for serving sizes. Little data exists on the variations of portion size norms of discretionary foods across settings. This study aimed to examine the differences in the range of normal portion sizes of commonly consumed foods between home and out-of-home settings (coffee shops, restaurants). Methods: A repeated cross-sectional design was used, with nine selected discretionary foods and beverages included in a validated online image-series questionnaire. Participants completed the questionnaire at two time points to report their normal portion sizes in home and out-of-home settings. Quantile regression models were used to examine differences in the range of normal portion sizes (17th to 83rd percentile, representing the majority of the study population) between settings. Results: A final sample of 295 participants was included in the analysis (51% females, mean age 40 ± 14 years). The ranges of normal portion sizes did not differ by settings for all test foods except for sugar-sweetened beverages (SSB) tested in both containers and glassware. SSB showed smaller normal portion sizes at home compared to fast food restaurants (in a bottle/can and in a glass/cup; p < 0.001). Conclusions: These findings suggest that the portion size norms of many discretionary foods are mostly consistent in home and out-of-home settings. As the typical serving sizes available to consumers in the out-of-home settings are large, it is essential to establish practical serving size guidelines directed at the food industry to increase the availability of smaller size options and empower consumers towards better portion control.
A Cross-Sectional Study of the Perception of Individualized Nursing Care Among Nurses in Acute Medical and Perioperative Settings
Authors: Ramos Ana , Pires Sara , Sá Eunice , Gomes Idalina , Alves Elisabete , Fonseca César , Coelho Anabela
Published: October 2024

Background/Objectives: Individualized nursing care allows for systematic assessment and intervention; considers a patient’s preferences, values, and context; and contributes to a positive care trajectory. However, its operationalization has proven to be challenging. This research aimed to evaluate nurses’ perceptions of individualized care and analyze their relationship with sociodemographic variables. Methods: A cross-sectional study was conducted on 122 eligible and registered nurses at a Hospital Center, in the Ophthalmology (operating room and inpatient ward) service, the Cardiology service, the Internal Medicine service, and the Medical Emergency Unit, for adults/older adults in Portugal. The nursing version of the Individualized Care Scale (ICS-Nurse) was used for the assessment, including three sub-dimensions: clinical situation, personal life situation, and decisional control over care-related decisions. Cronbach’s alpha and principal component analysis were used for the data analysis. The STROBE checklist was used to report this study. Results: No statistically significant differences were found based on the age, gender, level of education, or years of professional experience of the nurses within the sub-dimensions of individualization. The nurses overall had a good perception of the importance of individualized care (4.06 ± 0.46 ICS-A-NURSE) but faced difficulties in its implementation during the last shift they worked (3.97 ± 0.49 ICS-B-NURSE). Conclusions: The items considered to be of greatest importance were the response to the physical and emotional needs arising from illness and assistance in decision-making through educational instructions. Aspects related to the personal lives of patients, such as family inclusion in an individual’s care plan, everyday habits, and previous experiences of hospitalization, received the lowest scores. Recognizing priority areas for improvement in the individualization of nursing care can contribute to developing training programs and policies that promote a holistic approach. Future studies should consider patient outcomes related to their needs for individualization.
Knowledge and Consequences of Violence Against Health Professionals in Southern Portugal
Authors: Zangão Maria Otília , Gemito Laurência , Serra Isaura , Cruz Dulce , Barros Maria da Luz , Chora Maria Antónia , Santos Carolina , Coelho Anabela , Alves Elisabete
Published: October 2024

Background: Violence against health professionals is a global and growing problem, with significant impacts on the quality of care and the mental health of workers. Objectives: To analyze the level of knowledge, reporting practices and consequences of violence against health professionals in the Alentejo region (southern Portugal). Methods: This was an observational, cross-sectional study involving 440 health professionals (doctors and nurses). Data were collected using an online platform and a structured questionnaire created specifically for this study. In the statistical analysis, the data were described as counts and proportions and the X2 test was used considering a significance level of 0.05. Results: This study reveals that violence against health professionals in the southern region of Portugal is a frequent problem (40%), with a higher incidence among nurses (80%). Despite awareness of the existence and functioning of reporting channels, reporting is low (52%). The main causes are related to the health system, professionals and users. The consequences include mental health problems and a reduction in the quality of care provided. Suggested measures to combat violence include improving security, training and punishing aggressors. Conclusions: This study reveals that violence against health professionals in the southern region of Portugal is a frequent, under-reported problem with serious consequences for professionals and the quality of care.
Effect of Hypoxia on Siglec-7 and Siglec-9 Receptors and Sialoglycan Ligands and Impact of Their Targeting on NK Cell Cytotoxicity
Authors: Nawafleh Husam , Zeinelabdin Nagwa , Greene Michelle K. , Krishnan Anitha , Ho Linus , Genead Mohamed , Kunimoto Derek , Scott Christopher J. , Tolentino Michael , Chouaib Salem
Published: October 2024

Background/Objectives: Tumor microenvironmental hypoxia is an established hallmark of solid tumors. It significantly contributes to tumor aggressiveness and therapy resistance and has been reported to affect the balance of activating/inhibitory surface receptors’ expression and activity on NK cells. In the current study, we investigated the impact of hypoxia on the surface expression of Siglec-7 and Siglec-9 (Sig-7/9) and their ligands in NK cells and tumor target cells. The functional consequence of Siglec blockage using nanoparticles specifically designed to target and block Sig-7/9 receptors on NK cell cytotoxicity was elucidated. Methods: CD56⁺ CD3− NK cells were isolated from PBMCs along with an NK-92 clone and used as effector cells, while MCF-7 and K562 served as target cells. All cells were incubated under normoxic or hypoxic conditions for 24 h. To assess Siglec-7 and Siglec-9 receptor expression, U937, NK-92, and primary NK cells were stained with PE-labeled antibodies against CD328 Siglec-7/9. Interactions between Siglec-7/9 and their sialylated ligands, along with their functional impact on NK cell activity, were evaluated using polymeric nanoparticles coated with a sialic acid mimetic. Immunological synapse formation and live-cell imaging were performed with a ZEISS LSM 800 with Airyscan at 10× magnification for 24 h. Results: Our data indicate that hypoxia had no effect on the expression of Siglec-7/9 receptors by NK cells. In contrast, hypoxic stress resulted in an increase in Siglec-7 sialoglycan ligand expression by a sub-population of NK target cells. Using polymeric nanoparticles coated with a sialic acid mimetic that binds both Siglec-7 and -9 (Sig-7/9 NP), we demonstrated that incubation of these nanoparticles with NK cells resulted in increased immunological synapse formation, granzyme B accumulation, and killing of NK target cells. These studies indicate that hypoxic stress may have an impact on NK cell-based therapies and highlight the need to consider the hypoxic microenvironment for tumor-specific glycosylation. Conclusions: Our findings point to the role of Siglec–sialylated glycan interactions in hypoxic stress-induced NK cell dysfunction and recommend the potential integration of the manipulation of this axis through the targeting of Siglecs in future cancer immunotherapy strategies.
Lipid-Based Nanoformulations for Drug Delivery: An Ongoing Perspective
Authors: Rehman Mubashar , Tahir Nayab , Sohail Muhammad Farhan , Qadri Muhammad Usman , Duarte Sofia O. D. , Brandão Pedro , Esteves Teresa , Javed Ibrahim , Fonte Pedro
Published: October 2024

Oils and lipids help make water-insoluble drugs soluble by dispersing them in an aqueous medium with the help of a surfactant and enabling their absorption across the gut barrier. The emergence of microemulsions (thermodynamically stable), nanoemulsions (kinetically stable), and self-emulsifying drug delivery systems added unique characteristics that make them suitable for prolonged storage and controlled release. In the 1990s, solid-phase lipids were introduced to reduce drug leakage from nanoparticles and prolong drug release. Manipulating the structure of emulsions and solid lipid nanoparticles has enabled multifunctional nanoparticles and the loading of therapeutic macromolecules such as proteins, nucleic acid, vaccines, etc. Phospholipids and surfactants with a well-defined polar head and carbon chain have been used to prepare bilayer vesicles known as liposomes and niosomes, respectively. The increasing knowledge of targeting ligands and external factors to gain control over pharmacokinetics and the ever-increasing number of synthetic lipids are expected to make lipid nanoparticles and vesicular systems a preferred choice for the encapsulation and targeted delivery of therapeutic agents. This review discusses different lipids and oil-based nanoparticulate systems for the delivery of water-insoluble drugs. The salient features of each system are highlighted, and special emphasis is given to studies that compare them.
Toxicity and Dermatokinetic Analysis of Ibrutinib in Human Skin Models
Authors: Souto-Silva Maria Victória , Bispo Elizabete C. I. , Albuquerque Lucas F. F. , Barcelos Stefhani , Garcez Emãnuella M. , Quilici Luana S. , Neto Florêncio Figueiredo Cavalcanti , Duarte Eliza Carla Barroso , Boeloni Jankerle N. , Saldanha-Araujo Felipe , Gelfuso Guilherme M. , Carvalho Juliana Lott
Published: October 2024

Background/Objectives: Ibrutinib (IBR) is a tyrosine kinase inhibitor under investigation in preclinical and clinical settings as an alternative treatment for melanoma. Nevertheless, the limited oral bioavailability of IBR and the need for high doses of the drug to kill melanoma cells are major drawbacks for this purpose. Considering that melanoma is restricted to the skin at early stages, the topical application of IBR might constitute an effective and safer administration route. In this study, we determined IBR’s toxicity and dermatokinetics using human primary cells and human organotypic skin explant cultures (hOSECs). Methods: After demonstrating that human primary fibroblasts and keratinocytes present IBR target genes, the cytotoxicity of the drug was determined using the MTT and annexin V/PI staining assays. IBR toxicity in the skin was assessed using the TTC assay, and the irritation potential was established using histological assessment. Finally, IBR cutaneous permeation was assessed ex vivo to determine the drug dermatokinetics. Results: Our findings reveal that IBR exerts dose-dependent toxicity towards skin cells, presenting an IC50 in the same range as melanoma cells. The topical application of the drug successfully reduced irritation and toxicity in the skin, and the drug was shown to successfully permeate the stratum corneum and reach the viable skin layers in therapeutic concentrations. Conclusions: Overall, our data encourage the topical application of IBR to treat melanoma, paving the way for future studies in this theme.
8-Anilino-1-naphthalenesulfonate-Conjugated Carbon-Coated Ferrite Nanodots for Fluoromagnetic Imaging, Smart Drug Delivery, and Biomolecular Sensing
Authors: Thirumalai Anbazhagan , Girigoswami Koyeli , Prabhu Alex Daniel , Durgadevi Pazhani , Kiran Venkatakrishnan , Girigoswami Agnishwar
Published: October 2024

Background: Superparamagnetic properties and excitation independence have been incorporated into carbon-decorated manganese ferrite nanodots (MnFe@C) to introduce an economical and safer multimodal agent for use in both T1-T2 MRI and fluorescence-based imaging to replace the conventional highly toxic heavy metal contrast agents. Methods: The surface conjugation of 8-anilino-1-naphthalenesulfonate (ANS) to MnFe@C nanodots (ANS-MnFe@C) enhances both longitudinal and transverse MRI relaxation, improves fluorescence for optical imaging, and increases protein detection sensitivity, showing higher multimodal efficacy in terms of molar relaxivity, radiant efficiencies, and fluorescence sensitivity compared to MnFe@C. Results: The band gap energy was determined using Tauc’s equation to be 3.32 eV, while a 72% quantum yield demonstrated that ANS-MnFe@C was highly fluorescent, with the linear range and association constant calculated using the Stern–Volmer relation. The synthesized ANS-MnFe@C demonstrated excellent selectivity and sensitivity for bovine serum albumin (BSA), with a nanomolar detection limit of 367.09 nM and a broad linear range from 0.015 to 0.225 mM. Conclusions: In conclusion, ANS-MnFe@C holds ease of fabrication, good biocompatibility, as assessed in A375 cells, and an effective pH-sensitive doxorubicin release profile to establish anticancer activity in lung cancer cell line (A549), highlighting its potential as an affordable therapeutic agent for multimodal imaging, drug delivery, and protein sensing.
Nanocarrier-Based Transdermal Drug Delivery Systems for Dermatological Therapy
Authors: Kang Yunxiang , Zhang Sunxin , Wang Guoqi , Yan Ziwei , Wu Guyuan , Tang Lu , Wang Wei
Published: October 2024

Dermatoses are among the most prevalent non-fatal conditions worldwide. Given this context, it is imperative to introduce safe and effective dermatological treatments to address the diverse needs and concerns of individuals. Transdermal delivery technology offers a promising alternative compared to traditional administration methods such as oral or injection routes. Therefore, this review focuses on the recent achievements of nanocarrier-based transdermal delivery technology for dermatological therapy, which summarizes diverse delivery strategies to enhance skin penetration using various nanocarriers including vesicular nanocarriers, lipid-based nanocarriers, emulsion-based nanocarriers, and polymeric nanocarrier according to the pathogenesis of common dermatoses. The fundamentals of transdermal delivery including skin physiology structure and routes of penetration are introduced. Moreover, mechanisms to enhance skin penetration due to the utilization of nanocarriers such as skin hydration, system deformability, disruption of the stratum corneum, surface charge, and tunable particle size are outlined as well.
Pharmacometabolomics Approach to Explore Pharmacokinetic Variation and Clinical Characteristics of a Single Dose of Desvenlafaxine in Healthy Volunteers
Authors: Silveira Anne Michelli Reis , Sánchez-Vinces Salvador , Silva Alex Ap. Rosini , Sánchez-Luquez Karen , Garcia Pedro Henrique Dias , de Moura Garcia Caroline , de Brito Rhubia Bethania Socorro Lemos , Vieira Ana Lais , de Carvalho Lucas Miguel , Antonio Marcia Ap. , Carvalho Patrícia de Oliveira
Published: October 2024

This study investigated the effects of a single dose of desvenlafaxine via oral administration on the pharmacokinetic parameters and clinical and laboratory characteristics in healthy volunteers using a pharmacometabolomics approach. In order to optimize desvenlafaxine’s therapeutic use and minimize potential adverse effects, this knowledge is essential. Methods: Thirty-five healthy volunteers were enrolled after a health trial and received a single dose of desvenlafaxine (Pristiq®, 100 mg). First, liquid chromatography coupled to tandem mass spectrometry was used to determine the main pharmacokinetic parameters. Next, ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry was used to identify plasma metabolites with different relative abundances in the metabolome at pre-dose and when the desvenlafaxine peak plasma concentration was reached (pre-dose vs. post-dose). Results: Correlations were observed between metabolomic profiles, such as tyrosine, sphingosine 1-phosphate, and pharmacokinetic parameters, as well as acetoacetic acid and uridine diphosphate glucose associated with clinical characteristics. Our findings suggest that desvenlafaxine may have a broader effect than previously thought by acting on the proteins responsible for the transport of various molecules at the cellular level, such as the solute carrier SLC and adenosine triphosphate synthase binding cassette ABC transporters. Both of these molecules have been associated with PK parameters and adverse events in our study. Conclusions: This altered transporter activity may be related to the reported side effects of desvenlafaxine, such as changes in blood pressure and liver function. This finding may be part of the explanation as to why people respond differently to the drug.
The Uses of Phenomenology for Latinx Feminisms: Developing a Phenomenological Approach Informed by Rupture
Authors: Grimm Erika
Published: October 2024

Given the various shortcomings of classical phenomenological methods identified by critical and liberatory theorists, this paper considers what phenomenology has to offer theorists of multiply marginalized experience. The paper begins with an account of the major reasons for which Latinx feminists such as Linda Martín Alcoff, Jacqueline Martinez, and Mariana Ortega have found a phenomenological approach useful in their projects. This account reveals that though Latinx feminist phenomenologists have found useful resources for theorizing multiply marginalized theory and identity in the classical texts of phenomenology, experiences unique to those subjected to marginalization remain significantly underdeveloped or absent from classical accounts. Taking seriously the primacy of lived experiences of ‘rupture’, this paper argues, is therefore necessary in the development of a phenomenological approach that does justice to life in the borderlands and the lived experience of being-between-worlds. Informed by the work of Latinx feminist theorists such as Gloria Anzaldúa and María Lugones, this paper closes with a proposed critical feminist phenomenological approach that centers the moment of ‘rupture’ described in such work. Ultimately, this paper argues that the communication and documentation of these ruptures in the form of phenomenological description allows for the examination and interrogation of sedimented logics of oppression on the way to liberatory ends.
D-Band 4.6 km 2 × 2 MIMO Photonic-Assisted Terahertz Wireless Communication Utilizing Iterative Pruning Deep Neural Network-Based Nonlinear Equalization
Authors: Lin Jingwen , Xu Sicong , Wang Qihang , Zhang Jie , Ge Jingtao , Wang Siqi , Ou Zhihang , Ma Yuan , Zhou Wen , Yu Jianjun
Published: October 2024

In this paper, we explore the enhancement of a 4.6 km dual-polarization 2 × 2 MIMO D-band photonic-assisted terahertz communication system using iterative pruning-based deep neural network (DNN) nonlinear equalization techniques. The system employs advanced digital signal processing (DSP) methods, including down-conversion, resampling, matched filtering, and various equalization algorithms to combat signal distortions. We demonstrate the effectiveness of DNN and iterative pruning techniques in significantly reducing bit error rates (BERs) across a range of symbol rates (10 Gbaud to 30 Gbaud) and polarization states (vertical and horizontal). Before pruning, at 10 GBaud transmission, the lowest BER was 0.0362, and at 30 GBaud transmission, the lowest BER was 0.1826, both of which did not meet the 20% soft-decision forward error correction (SD-FEC) threshold. After pruning, the BER at different transmission rates was reduced to below the hard decision forward error correction (HD-FEC) threshold, indicating a substantial improvement in signal quality. Additionally, the pruning process contributed to a decrease in network complexity, with a maximum reduction of 85.9% for 10 GBaud signals and 63.0% for 30 GBaud signals. These findings indicate the potential of DNN and pruning techniques to enhance the performance and efficiency of terahertz communication systems, providing valuable insights for future high-capacity, long-distance wireless networks.
Adaptive Threshold Algorithm for Outlier Elimination in 3D Topography Data of Metal Additive Manufactured Surfaces Obtained from Focus Variation Microscopy
Authors: Xu Xin , Pahl Tobias , Hagemeier Sebastian , Lehmann Peter
Published: October 2024

The topography of surfaces produced by metal additive manufacturing is a challenge for optical measurement systems such as focus variation microscopes. These irregularities can lead to artifacts, such as incorrectly measured protrusions or spikes, hampering reliable topographic characterization. In order to eliminate this problem, we introduce a new algorithm based on dual convolving a vertical Sobel operator with cross sections of an image stack parallel to the scanning direction of the so-called depth scan. This has proven beneficial in order to distinguish the focus region from out-of-focus areas where outliers are frequently detected. This paper introduces a method for deriving self-adaptive thresholds from the convolution result and compares the effects of different operators in creating self-adaptive thresholds. Additionally, a simulation model of focus variation microscopy is introduced to validate both the measuring system and the proposed algorithm, thereby enhancing the overall performance of focus variation microscopy. Finally, comparisons of measurement results on rough metal additive manufacturing workpieces with and without self-adaptive thresholds are discussed to demonstrate the algorithm’s effectiveness.The utilization of self-adaptive thresholds demonstrably reduces the uncertainty range in roughness parameter calculations. For example, in the case of an additive manufactured metal sample due to outlier elimination, the Sz roughness value reduces from 543 µm to 413 µm.
Cylindrical Vector Beams with an MOPA Amplifier Based on Nonlinear Polarization Rotation Mode-Locking
Authors: Zhang Tianyu , Li Dong , Pei Siqi , Zhu Yun , Hu Jiapeng , Liu Xuesheng , Yan Anru , Liu Youqiang , Wang Zhiyong
Published: October 2024

CVBs (cylindrical vector beams) are widely used in optical imaging, optical trapping, material processing, etc. In this study, based on mode-selective couplers and passive mode-locking fiber technology, a cylindrical vector fiber amplifier with an MOPA (master oscillator power amplifier) structure was developed. In the experiment, the pre-amp stage reached 19.87 mW output power and a CVB output with a mode purity greater than 97%. The measured beam quality factor was M2 = 2.1. The CVB output power obtained by the first-amp stage was 152.4 mW, and the mode purity was greater than 92%. The measured beam quality factor was M2 = 1.99. The internal inhomogeneity and external effects of the isotropic LMA (large-mode-area) fiber led to a decrease in beam quality and mode purity. After amplification, the gain of the fundamental mode was higher and the power was greater, resulting in a greatly reduced mode purity. This CVB fiber amplifier yielded important research value in expanding the applications of high-power fiber lasers.
The Design of Highly Reflective All-Dielectric Metasurfaces Based on Diamond Resonators
Authors: Xing Zhongyang , Liao Jiahui , Xu Zhongjie , Cheng Xiang’ai , Zhang Jiangbin
Published: October 2024

All-dielectric metasurfaces offer a low-loss alternative to plasmonic metasurfaces. We proposed the configuration for high-reflectivity all-dielectric metasurfaces based on single-crystal diamond (SCD) resonators on fused silica substrate and conducted simulations to optimize and analyze such a configuration via the FDTD solver. We utilized GMR as the design principle to select the configuration and the substrate material, and analyzed the scattering properties of a single SCD resonator by multipole decomposition. Then, we demonstrated that both the cylindrical resonators in square lattice and frustum-shaped resonators in hexagonal lattice can achieve near-unity reflectivity (>99.99%) and ultra-low absorption (<0.001%) at 795 nm, the typical alkali-metal laser wavelength. Additionally, we demonstrated that such a design is quite tolerant of fabrication errors and further supports its potential for realistic applications. To expand the functionality of such devices across multiple wavelengths, dual-band high-reflectivity metasurfaces at 744 nm and 828 nm were also designed. Our work is quite useful for designing diamond-based highly reflective mirrors, paving the way for low-loss all-dielectric reflective metasurfaces in high-power laser applications.
Anticancer Potential of Tocopherols-Containing Plants and Semi-Synthetic Tocopherols
Authors: Sianipar Nesti Fronika , Muflikhati Zidni , Mangindaan Dave , Assidqi Khoirunnisa
Published: October 2024

Tocopherols, potent bioactive compounds with anticancer properties, remain understudied in herbal medicinal plants, presenting a significant knowledge gap in the field of natural anticancer agents. This review evaluates tocopherol-containing plants for their anticancer potential, analyzing Scopus publications from 2016 to 2024. Fifteen herbal medicinal plants were identified as promising candidates, including Bulbine anguistifolia Poelln, Punica granatum L., Moringa oleifera, Kigelia pinnata, and Typhonium flagelliforme Lodd. The review explores tocopherols’ anticancer mechanisms, including apoptosis induction and cell cycle arrest. Factors influencing tocopherols’ anticancer effects are examined, such as their forms (α, β, γ, δ), concentrations, plant parts utilized, and their stability in various plants. Additionally, emerging research on semi-synthetic tocopherol derivatives is analyzed, highlighting their potential as adjuvants in chemotherapy and their role in enhancing drug delivery and reducing side effects. This comprehensive analysis aims to advance the development of plant-based anticancer pharmaceuticals and improve cancer treatment strategies. By elucidating the mechanisms and potential of tocopherol-containing plants, this review provides a foundation for future research in plant-based anticancer therapies. It emphasizes the need for further investigation into these plants’ anticancer properties, potentially leading to novel, more effective, and less toxic cancer therapies. The findings presented here contribute to a nuanced understanding of how tocopherol-containing plants can be leveraged in the development of future anticancer drugs.
Altered Metabolism in Knockdown Lines of Two HXXXD/BAHD Acyltransferases During Wound Healing in Potato Tubers
Authors: Sinka Jessica L. , Queralta-Castillo Indira , Yeung Lorena S. , Molina Isabel , Dhaubhadel Sangeeta , Bernards Mark A.
Published: October 2024

Suberin biosynthesis involves the coordinated regulation of both phenolic and aliphatic metabolisms. HXXXD/BAHD acyltransferases occupy a unique place in suberization, as they function to crosslink phenolic and aliphatic monomers during suberin assembly. To date, only one suberin-associated HXXXD/BAHD acyltransferase, StFHT, has been described in potatoes, whereas, in Arabidopsis, at least two are implicated in suberin biosynthesis. RNAseq data from wound-induced potato tubers undergoing suberization indicate that transcripts for 28 HXXXD/BAHD acyltransferase genes accumulate in response to wounding. In the present study, we generated RNAi knockdown lines for StFHT and another highly wound-induced HXXXD/BAHD acyltransferase, designated StHCT, and characterized their wound-induced suberin phenotype. StFHT-RNAi and StHCT-RNAi knockdown lines share the same aliphatic suberin phenotype of reduced esterified ferulic acid and ferulates, which is similar to the previously described StFHT-RNAi knockdown suberin phenotype. However, the phenolic suberin phenotype differed between the two knockdown genotypes, with StHCT-RNAi knockdown lines having proportionately more p-hydroxyphenyl-derived moieties than either StFHT-RNAi knockdown or empty vector control lines. Analysis of soluble polar metabolites revealed that StHCT catalyzes a step upstream from StFHT. Overall, our data support the involvement of more than one HXXXD/BAHD acyltransferase in potato suberin biosynthesis.
Extracellular DNA as a Strategy to Manage Vascular Wilt Caused by Fusarium oxysporum in Tomato (Solanum lycopersicum L.) Based on Its Action as a Damage-Associated Molecular Pattern (DAMP) or Pathogen-Associated Molecular Pattern (PAMP)
Authors: Jiménez-Hernández Alejandra , Carbajal-Valenzuela Ireri Alejandra , Torres-Pacheco Irineo , Rico-García Enrique , Ocampo-Velazquez Rosalía V. , Feregrino-Pérez Ana Angélica , Guevara-Gonzalez Ramón Gerardo
Published: October 2024

Vascular wilt is an important tomato disease that affects culture yields worldwide, with Fusarium oxysporum (F.o) being the causal agent of this infection. Several management strategies have lost effectiveness due to the ability of this pathogen to persist in soil and its progress in vascular tissues. However, nowadays, research has focused on understanding the plant defense mechanisms to cope with plant diseases. One recent and promising approach is the use of extracellular DNA (eDNA) based on the ability of plants to detect their self-eDNA as damage-associated molecular patterns (DAMPs) and pathogens’ (non-self) eDNA as pathogen-associated molecular patterns (PAMPs). The aim of this work was to evaluate the effect of the eDNA of F.o (as a DAMP for the fungus and a PAMP for tomato plants) applied on soil, and of tomato’s eDNA (as a DAMP of tomato plants) sprayed onto tomato plants, to cope with the disease. Our results suggested that applications of the eDNA of F.o (500 ng/µL) as a DAMP for this pathogen in soil offered an alternative for the management of the disease, displaying significantly lower disease severity levels in tomato, increasing the content of some phenylpropanoids, and positively regulating the expression of some defense genes. Thus, the eDNA of F.o applied in soil was shown to be an interesting strategy to be further evaluated as a new element within the integrated management of vascular wilt in tomato.
Implementing Real-Time Image Processing for Radish Disease Detection Using Hybrid Attention Mechanisms
Authors: Ji Mengxue , Zhou Zizhe , Wang Xinyue , Tang Weidong , Li Yan , Wang Yilin , Zhou Chaoyu , Lv Chunli
Published: October 2024

This paper developed a radish disease detection system based on a hybrid attention mechanism, significantly enhancing the precision and real-time performance in identifying disease characteristics. By integrating spatial and channel attentions, this system demonstrated superior performance across numerous metrics, particularly achieving 93% precision and 91% accuracy in detecting radish virus disease, outperforming existing technologies. Additionally, the introduction of the hybrid attention mechanism proved its superiority in ablation experiments, showing higher performance compared to standard self-attention and the convolutional block attention module. The study also introduced a hybrid loss function that combines cross-entropy loss and Dice loss, effectively addressing the issue of class imbalance and further enhancing the detection capability for rare diseases. These experimental results not only validate the effectiveness of the proposed method, but also provide robust technical support for the rapid and accurate detection of radish diseases, demonstrating its vast potential in agricultural applications. Future research will continue to optimize the model structure and computational efficiency to accommodate a broader range of agricultural disease detection needs.
Mn3O4 Nanoenzyme Seed Soaking Enhanced Salt Tolerance in Soybean Through Modulating Homeostasis of Reactive Oxygen Species and ATPase Activities
Authors: Mao Tingyong , Bao Linfeng , Zhang Hengbin , Shi Zhilin , Liu Jiahao , Wang Desheng , Liu Chan , Zhan Yong , Zhai Yunlong
Published: October 2024

Soybean, an important cash crop, is often affected by soil salinity, which is one of the important types of abiotic stress that affects its growth. Poly (acrylic) acid coated Mn3O4 (PMO) has been reported to play a vital role in defending against a variety of abiotic stresses in plants. To date, the effects of PMOs on soybean have not been reported; this study explored the mechanism of PMO-enhanced soybean germination under salt stress. In this experiment, 100 mg/L PMO was used as an immersion agent with a salt treatment of 150 mM NaCl. The results showed that when compared with the PMO treatment, salt stress significantly decreased the germination rate, fresh weight, carbohydrate content, and antioxidant enzyme activity of soybean and significantly increased the contents of reactive oxygen species, malondialdehyde, and osmoregulatory substances. However, PMO treatment enhanced the antioxidant defense system and significantly reduced the malondialdehyde content of soybean. Moreover, the activities of H+-ATPase and Ca2+-ATPase were significantly higher in treated soybean than in the control, and the content of ATP was also higher in treated soybean than in the control. Generally, PMO regulates the homeostasis of reactive oxygen species and reduces ATP consumption, thereby improving the ability of soybeans to germinate under salt stress. This study provides new insights into how nanomaterials improve plant salt tolerance.
Floral Elegance Meets Medicinal Marvels: Traditional Uses, Phytochemistry, and Pharmacology of the Genus Lagerstroemia L.
Authors: Yue Ziwei , Xu Yan , Cai Ming , Fan Xiaohui , Pan Huitang , Zhang Donglin , Zhang Qixiang
Published: October 2024

The genus Lagerstroemia L. (Lythraceae), known for its exquisite flowers and prolonged flowering period, is commonly employed in traditional medicinal systems across Asian countries, where it has always been consumed as tea or employed to address ailments such as diabetes, urinary disorders, coughs, fevers, inflammation, pain, and anesthesia. Its diverse uses may be attributed to its rich active ingredients. Currently, at least 364 biological compounds have been identified from Lagerstroemia extracts, encompassing various types such as terpenes, flavonoids, phenolic acids, alkaloids, and phenylpropanoids. Extensive in vitro and in vivo experiments have examined the pharmacological activities of different extracts, revealing their potential in various domains, including but not limited to antidiabetic, anti-obesity, antitumor, antimicrobial, antioxidant, anti-inflammatory, analgesic, and hepatoprotective effects. Additionally, 20 core components have been proven to be associated with antidiabetic and hypoglycemic effects of Lagerstroemia. Overall, Lagerstroemia exhibit substantial medicinal potential, and the alignment between its traditional applications and contemporary pharmacological findings present promising opportunities for further investigation, particularly in food and health products, drug development, herbal teas, and cosmetics. However, evidence-based pharmacological research has largely been confined to in vitro screening and animal model, lacking clinical trials and bioactive compound isolations. Consequently, future endeavors should adopt a more holistic approach.
Experimental Investigation on the Mechanical and Dynamic Thermomechanical Properties of Polyether Ether Ketone Based on Fused Deposition Modeling
Authors: Liu Guocheng , Hu Ning , Huang Junjie , Tu Qiyong , Xu Fengxiang
Published: October 2024

In this work, the mechanical and dynamic thermomechanical properties of PEEK based on FDM are experimentally investigated and evaluated comprehensively. The tensile failure mechanism of PEEK prepared by FDM and extrusion modeling (EM) was analyzed by fracture morphology observation. By conducting a differential scanning calorimetry (DSC) test, the crystallinity of PEEK prepared by FDM and EM was measured. The dynamic thermomechanical properties of PEEK were tested and analyzed by dynamic mechanical analysis (DMA). For FDM-prepared PEEK samples, the yield strength and elongation were 98.3 ± 0.49 MPa and 22.86 ± 2.12%, respectively. Compared with the yield strength of PEEK prepared by EM, the yield strength of PEEK prepared by FDM increased by 65.38%. The crystallinity of FDM-prepared and EM-prepared samples was calculated as 34.81% and 31.55%, respectively. Different processing methods resulted in differences in the microscopic morphology and crystallinity of two types of PEEK parts, leading to differences in mechanical properties. The internal micropores generated during the FDM processing of PEEK significantly reduced the elongation. Moreover, according to the DMA results, the glass transition activation energy of PEEK was obtained as ΔE = 685.07 kJ/mol based on the Arrhenius equation. Due to the excellent mechanical properties of PEEK prepared by FDM processing, it is promising for high-performance polymer applications in different fields.
Textile Recycling: Efficient Polyester Recovery from Polycotton Blends Using the Heated High-Ethanol Alkaline Aqueous Process
Authors: Pavlopoulou Kalliopi Elli , Hrůzová Kateřina , Kahoush May , Kadi Nawar , Patel Alok , Rova Ulrika , Matsakas Leonidas , Christakopoulos Paul
Published: October 2024

Textile production has doubled in the last 20 years, but only 1% is recycled into new fibers. It is the third largest contributor to water pollution and land use, accounting for 10% of global carbon emissions and 20% of clean water pollution. A key challenge in textile recycling is blended yarns, such as polycotton blends, which consist of polyester and cotton. Chemical recycling offers a solution, in particular, alkali treatment, which hydrolyzes polyester (PET) into its components while preserving cotton fibers. However, conventional methods require high temperatures, long durations, or catalysts. Our study presents, for the first time, the heated high-ethanol alkaline aqueous (HHeAA) process that efficiently hydrolyzes PET from polycotton at lower temperatures and without a catalyst. A near-complete PET hydrolysis was achieved in 20 min at 90 °C, while similar results were obtained at 70 °C and 80 °C with longer reaction times. The process was successfully scaled at 90 °C for 20 min, and complete PET hydrolysis was achieved, with a significantly reduced liquid-to-solid ratio, from 40 to 7 (L per kg), signifying its potential to be implemented in an industrial context. Additionally, the cotton maintained most of its properties after the treatment. This method provides a more sustainable and efficient approach to polycotton recycling.
A Sustainable and Eco-Friendly Membrane for PEM Fuel Cells Using Bacterial Cellulose
Authors: Yang Xiaozhen , Huang Lin , Deng Qiang , Dong Weifu
Published: October 2024

Bacterial cellulose (BC) is an advantageous polymer due to its renewable nature, low cost, environmental compatibility, biocompatibility, biodegradability, chemical stability, and ease of modification. With these advantages, BC is an interesting candidate for the development of novel eco-friendly materials for proton-exchange membrane (PEM) applications. However, its practical applications have been limited by its relatively high dispersion in water, which usually occurs during the operation of proton-exchange membrane fuel cells (PEMFCs). In addition, the proton conductivity of bacterial cellulose is poor. In this study, functionalized BC modified with 3-aminopropyltriethoxysilane (APTES) was prepared using a solvent casting method to enhance its performance. The results showed that the water stability of the modified BC membrane was significantly improved, with the contact angle increasing from 54.9° to 103.3°. Furthermore, the optimum ratio of BC and APTES was used to prepare a proton-exchange membrane with a maximum proton conductivity of 62.2 mS/cm, which exhibited a power generation performance of 4.85 mW/cm2 in PEMFCs. It is worth mentioning that modified BC membranes obtained by combining an alkaline proton carrier (-NH2) with BC have rarely been reported. As fully bio-based conductive membranes for PEMFCs, they have the potential to be a low-cost, eco-friendly, and degradable alternative to expensive, ecologically problematic fluoric ionomers in short-term or disposable applications, such as biodegradable electronics and portable power supplies.
Enhancing EDM Productivity for Plastic Injection Mold Manufacturing: An Experimental Optimization Study
Authors: Titu Aurel Mihail , Pop Alina Bianca
Published: October 2024

Electrical erosion molding (EDM) is an unconventional machining technology widely used in the manufacture of injection molds for plastics injection molding for the creation of complex cavities and geometries. However, EDM productivity can be challenging, directly influencing mold manufacturing time and cost. This work aims to improve EDM productivity in the context of mold manufacturing for plastics injection molding. The research focuses on the optimization of processing parameters and strategies to reduce manufacturing time and increase process efficiency. Through a rigorous experimental approach, this work demonstrates that the optimization of EDM parameters and strategies can lead to significant productivity gains in the manufacture of plastic injection molds without compromising part quality and accuracy. This research involved a series of controlled experiments on a Mitsubishi EA28V Advance die-sinking EDM machine. Different combinations of pre-cutting parameters and processing strategies were investigated using copper electrodes on a heat-treated steel plate. Productivity was evaluated by measuring the volume of material removed, and geometrical accuracy was checked on a coordinate measuring machine. The experimental results showed a significant increase in productivity (up to 61%) by using the “processing speed priority” function of the EDM machine, with minimal impact on geometric accuracy. Furthermore, the optimized parameters led to an average reduction of 12% in dimensional deviations, indicating improved geometric accuracy of the machined parts. This paper also provides practical recommendations on the selection of optimal EDM processing parameters and strategies, depending on the specific requirements of plastic injection mold manufacturing.
Improvement of Mechanical and Acoustic Characteristics of Halloysite Nanotube-Reinforced Polyurethane Elastomer Composites and Their Applications
Authors: Ge Mengchen , Li Xiaodong , Su Xing , Jiang Hao , Wang Yangwei , Han Fei , Zou Meishuai
Published: October 2024

Polyurethane incorporated with nanofillers such as carbon nanotubes, basalt fibers, and clay nanoparticles has presented remarkable potential for improving the performance of the polymeric composites. In this study, the halloysite nanofiller-reinforced polyurethane elastomer composites were prepared via the semi-prepolymer method. The impact of different halloysites (halloysite nanotubes and halloysite nanoplates) in polyurethane composites was investigated. Scanning electron microscopy, X-ray diffraction, infrared spectroscopy, electronic universal tensile testing, and acoustic impedance tube testing were employed to characterize the morphology, composition, phase separation, mechanical properties, and sound insulation of the samples. The composite fabricated with 0.5 wt% of halloysite nanotubes introduced during quasi-prepolymer preparation exhibited the highest tensile strength (22.92 ± 0.84 MPa) and elongation at break (576.67 ± 17.99%) among all the prepared samples. Also, the incorporation of 2 wt% halloysite nanotubes into the polyurethane matrix resulted in the most significant overall improvements, particularly in terms of tensile strength (~44%), elongation at break (~40%), and sound insulation (~25%) within the low-frequency range of 50 to 1600 Hz. The attainment of these impressive mechanical and acoustic characteristics could be attributed to the unique lumen structure of the halloysite nanotubes, good dispersion of the halloysites in the polyurethane, and the interfacial bonding between the matrix and halloysite fillers.
Investigating the Effect of Nano-Crystalline Cellulose in Nitrile Butadiene Rubber Matrix for Improved Thermo-Mechanical Properties
Authors: Nafees Asra , Hashmi Saud , Ahmed Rafiq
Published: October 2024

The escalating demand for sustainable rubber products has spurred research into alternative reinforcing fillers, driven by concerns regarding the detrimental effects of using conventional fillers like carbon black and silica. In this investigation, nano-crystalline cellulose (NCC), derived from micro crystalline cellulose (MCC), sourced from sugarcane bagasse via acid hydrolysis, serves as a bio-filler to reinforce Nitrile Butadiene Rubber (NBR) matrices. NBR-NCC nano-composites were prepared using a two-roll mill, varying NCC from 1–5 parts per hundred rubber matrices, followed by hot press curing. NCC and NBR-NCC nano-composites were characterized using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), curing characteristics, thermo-mechanical testing, thermal aging and motor oil resistance. Chemical interactions between the NCC and NBR matrix were verified with FTIR. The SEM images of the NCC showed a combination of rod-like and spherical morphologies and a homogenous dispersion of NCC in NBR-NCC nano-composites with some agglomeration, notably at higher percentages of NCC. It is shown that the cure time decreases with increasing NCC loading which mimics a shorter industrial production cycle. The results also showed an increase in tensile strength, hardness, oil resistance and a rise in degradation temperature when compared to NBR at approximately 34%, 36%, 38% and 32 °C, respectively, at 3 phr NCC loading. Furthermore, NBR-NCC nano-composites showed a lower decrease in mechanical properties after aging when compared to NBR. The findings of this research suggest that the NBR-NCC nano-composites may find applications in high oil resistance seals and rubber gloves where higher thermal stability is strictly required.