bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of Mental Health Complaints Among Performing Arts Students Is Associated With COVID-19 Preventive Measures
Authors: Stubbe Janine H. , Tiemens Annemiek , Keizer-Hulsebosch Stephanie C. , Steemers Suze , van Winden Diana , Buiten Maurice , Richardson Angelo , van Rijn Rogier M.
Published: June 2021

The aim of this study was to investigate the effect of COVID-19 preventive measures on the mental health of performing arts students. In a prospective cohort study, performing arts students (N= 213) from Codarts Rotterdam, University of the Arts, were invited to monitor their health during one academic year (September 2019–May 2020). Every month, students completed items on mental health complaints, stress, and sleep quality. Chi-square tests and repeated-measures ANOVA with deviation contrasts were performed to investigate whether COVID-19 preventive measures were associated with changes in mental health complaints, stress scores, and sleep quality. During the COVID-19 lockdown, subjective mental health, Mental Health Inventory-5 (MHI-5), and items on loneliness were additionally completed by the respondents. A total of 98 students (46.0%) were included in the analyses. The 3-month prevalence of mental health complaints was significantly higher during the COVID-19 lockdown compared to the two pre-COVID-19 periods (p< 0.001). Mean stress scores were significantly lower for February (35.20) and March (36.41) when compared to the overall mean (40.38). Sleep quality scores (mean) were significantly higher for April (6.90), and May (6.89) when compared to overall mean (6.58). Furthermore, at least 75.5% of the participants dealt with moderate to very severe loneliness in all 3 months during the COVID-19 lockdown. During lockdown, performing arts students perceived less stress and their sleep quality increased. However, the prevalence of mental health complaints increased. Besides, 3 out 4 students dealt with moderate to very severe loneliness.
Case Report: Anti-flotillin 1/2 Autoantibody-Associated Atypical Dementia
Authors: Hansen Niels , Bartels Claudia , Stöcker Winfried , Wiltfang Jens , Timäus Charles
Published: June 2021

Flotillin proteins are involved in neurodegeneration and T-cell immunity. Here, we report the case of 65-year-old woman who presented with dementia, depressive symptoms, and a patient history involving speech problems. As diagnostics methods we applied magnetic resonance imaging, clinical examination, extensive neuropsychological testing, and cerebrospinal fluid analysis. Neuropsychological testing revealed major cognitive decline in attentional, executive, and memory functions together with impaired activities of daily living. The cerebrospinal fluid showed elevated phosphorylated tau protein 181. We identified serum autoantibodies against the flotillin 1/2 complex. Immunotherapy entailing four cycles of high-dose steroids resulted in less cognitive dysfunction along with reduced depressive symptoms in the second follow-up after starting steroids. In conclusion: probable autoimmune-mediated dementia associated with anti-flotillin 1/2 complex autoantibodies expands the phenotypic spectrum of anti-flotillin 1/2 antibody disease.
Heart Rate Variability Duration: Expanding the Ability of Wearable Technology to Improve Outpatient Monitoring?
Authors: Sheridan David C. , Domingo Karyssa N. , Dehart Ryan , Baker Steven D.
Published: June 2021

Heart rate variability (HRV) evaluates beat-to-beat interval (BBI) differences and is a suggested marker of the autonomic nervous system with diagnostic/monitoring capabilities in mental health; especially parasympathetic measures. The standard duration for short-term HRV analysis ranges from 24 h down to 5-min. However, wearable technology, mainly wrist devices, have large amounts of motion at times resulting in need for shorter duration of monitoring. The objective of this study was to evaluate the correlation between 1 and 5 min segments of continuous HRV data collected simultaneously on the same patient. Subjects wore a patch electrocardiograph (Cardea Solo, Inc.) over a 1–7 day period. For every consecutive hour the patch was worn, we selected a 5-min, artifact-free electrocardiogram segment. HRV metric calculation was performed to the entire 5-min segment and the first 1-min from this same 5-min segment. There were 492 h of electrocardiogram data collected allowing calculation of 492 5 min and 1 min segments. 1 min segments of data showed good correlation to 5 min segments in both time and frequency domains: root mean square of successive difference (RMSSD) (R = 0.92), high frequency component (HF) (R = 0.90), low frequency component (LF) (R = 0.71), and standard deviation of NN intervals (SDNN) (R = 0.63). Mental health research focused on parasympathetic HRV metrics, HF and RMSSD, may be accomplished through smaller time windows of recording, making wearable technology possible for monitoring.
Association of Gender With Outcomes in Hospitalized Patients With 2019-nCoV Infection in Wuhan
Authors: Han Huiwu , Peng Xiaobei , Zheng Fan , Deng Guiyuan , Cheng Xiaocui , Peng Liming
Published: June 2021

Aim: The aim of this study was to analyze the association of gender with psychological status and clinical outcomes among patients with 2019-nCoV infection to provide new directions for the prevention and control of the pandemic.

Methods: One hundred and thirty-eight patients with confirmed 2019-nCoV infection at Wuhan Union Hospital, between February 8 and March 31, 2020, were included in the study analysis. General information and data on clinical characteristics were collected from patients' medical records. Participants' responses to self-report measures of psychological status were also collected.

Results: Anxiety levels, depression levels, and recovery rates were significantly higher among women compared to men. Conversely, chronic disease history and smoking rates, dry cough incidence, C-reactive protein levels, and disease severity were significantly higher among men than women (p < 0.05).

Conclusion: Female patients experienced more severe psychological issues, due to higher levels of anxiety and stress, than male patients; indicating that more attention should be paid to the psychological care of female patients. In contrast, the general condition of male patients was more severe, particularly among elderly male patients with a history of chronic disease and smoking, suggesting that, to prevent and control 2019-nCoV infection, male patients should be encouraged to quit smoking as soon as possible to reduce the risk of severe pneumonia.
Meta-Decision in Healthcare
Authors: Baynouna Al Ketbi Latifa Mohammad
Published: June 2021

Meta-decision as a junction between evidence and its rightful implementation is suggested in this review as a structured framework applied in healthcare, valuable to clinicians and healthcare decision-makers. The process of meta-decision requires optimum measurements to provide data necessary for identifying and developing decision alternatives and explicitly reflect on its value and choose the optimum decision. The location of value in the meta-decision framework is core component. Of equal importance are prerequisites for decision-makers' abilities to make meta-decisions and focus on optimum team environments. As well as improving their decision-making process through reflection and learning.
Constrained by Design: Influence of Genetic Encodings on Evolved Traits of Robots
Authors: Miras Karine
Published: June 2021

Genetic encodings and their particular properties are known to have a strong influence on the success of evolutionary systems. However, the literature has widely focused on studying the effects that encodings have on performance, i.e., fitness-oriented studies. Notably, this anchoring of the literature to performance is limiting, considering that performance provides bounded information about the behavior of a robot system. In this paper, we investigate how genetic encodings constrain the space of robot phenotypes and robot behavior. In summary, we demonstrate how two generative encodings of different nature lead to very different robots and discuss these differences. Our principal contributions are creating awareness about robot encoding biases, demonstrating how such biases affect evolved morphological, control, and behavioral traits, and finally scrutinizing the trade-offs among different biases.
Maternal Pre-conception Body Mass Index and Fasting Plasma Glucose With the Risk of Pre-term Birth: A Cohort Study Including 4.9 Million Chinese Women
Authors: Xu Qin , Zhou Qiongjie , Yang Ying , Liu Fangchao , Wang Long , Wang Qiaomei , Shen Haiping , Xu Zongyu , Zhang Yiping , Yan Donghai , Peng Zuoqi , He Yuan , Wang Yuanyuan , Zhang Ya , Zhang Hongguang , Ma Xu , Li Xiaotian
Published: June 2021

Background:To evaluate the associations of pre-conception body mass index (BMI), fasting plasma glucose (FPG) alone and their combination with pre-term birth (PTB) risk.

Methods:We conducted a population-based retrospective cohort study with 4,987,129 reproductive-aged women, who participated in National Free Pre-Pregnancy Checkups Project in 2013–2016 and had a singleton delivery before December 2017 in China. All data analyses were conducted in 2018–2021.

Results:A total of 339,662 (6.81%) women had pre-term deliveries. Compared with women with normal weight and normal glucose, underweight and normal weight were associated with PTB among hypoglycemia women, the adjusted odd ratios (aORs) were 1.24 (95% CI: 1.05–1.48) and 1.16 (95% CI: 1.07–1.25), respectively; underweight, overweight and obesity were associated with PTB among women with normal glucose, the aORs were 1.09 (95% CI: 1.08–1.10), 1.06 (95% CI: 1.05–1.07) and 1.08 (95% CI: 1.05–1.12), respectively; all the BMI groups were significantly associated with PTB among women with pre-diabetes or diabetes (P< 0.05). The dose-response relationships of BMI with PTB varied in different FPG level, with U-shaped curve in normal glucose and pre-diabetes women, J-shaped in diabetes women, L-shaped in hypoglycemia women. For FPG with PTB, the dose-response relationships were U-shaped in normal weight, overweight, and obesity women, and L-shaped in underweight women.

Conclusion:We found that the associations of PTB with BMI varied with levels of FPG, and associations of PTB with FPG varied with levels of BMI. There was a synergistic effect on PTB risk due to abnormal weight and glycemia besides a conventional main effect derived from either of them. Achieving desirable weight and glucose control before conception should be advised.
Addressing Cancer Screening Inequities by Promoting Cancer Prevention Knowledge, Awareness, Self-Efficacy, and Screening Uptake Among Low-Income and Illiterate Immigrant Women in France
Authors: De Jesus Maria , Rodrigue Christelle M. , Rahmani Sarah , Balamou Christian
Published: June 2021

Objective: Cancer screening rates are suboptimal for disadvantaged populations in France, yet little evidence exists on their cancer-related knowledge and screening barriers. The main objective of this study was to examine cancer-related knowledge, awareness, self-efficacy, and perceptions of screening barriers among low-income, illiterate immigrant women in France following an 8-weeks cancer educational intervention.

Methods: Semi-structured qualitative interviews were conducted with 164 female participants in the Ain department of France between January 2019 and March 2020. Adopting the Health Belief Model as an intervention and analytic framework, salient themes were identified using qualitative thematic analysis.

Results: Increased levels of perceived susceptibility to and perceived severity of cancer contributed to higher motivation to get screened. Barriers to screening included low French proficiency, shame surrounding illiteracy, and constant worries due to precarious living conditions. Perceived benefits (e.g., valuing one’s health and health-promoting behaviors), cues to action from a trusted source, and greater self-efficacy (e.g., more autonomous in healthcare-seeking) outweighed perceived barriers, including cultural barriers.

Conclusions: Implications include developing audience-responsive targeted cancer screening communication strategies and educational materials to increase screening rates and reduce cancer and cancer screening inequities.
Soilless Cultivation System, Electrical Conductivity of Nutrient Solution, and Growing Season on Yield and Quality of Baby-Leaf Oak-Leaf Lettuce
Authors: Conversa Giulia , Bonasia Anna , Lazzizera Corrado , Elia Antonio
Published: June 2021

The floating system (FL) is a common soilless method for baby-leaf production, whereas the ebb and flow system (EF) has been proposed as an alternative. Both of them allow managing plant saline stress while preventing reduction in plant growth and yield and increasing product quality. The oak-leaf lettuce response to the growing conditions (hydroponics, salinity) in interaction with climate and genotype has been little studied. Two experiments were carried out with two oak-leaf cultivars (green- and red-colored type) grown in FL and EF systems at two levels of nutrient solution (NS) electrical conductivity (EC) (EC = 2.5 and 3.5 dS m−1; EC2.5, EC3.5, respectively) under autumn and late-spring conditions. The EF system caused an increase in salinity in the substrate where roots mainly develop, so it overcomes the effect of the EC3.5 treatment. In the autumn cycle, irrespective of the EC, EF-grown plants had improved leaf thickness (specific leaf area), color, and antioxidative (total phenol and carotenoid contents) properties; however, a reduction in yield was observed in the most productive cultivar (green type). In late spring, higher yield and product quality (processability, nitrate content) were obtained at the expense of color, with the FL showing the best productivity in the green type cultivar. The red type cultivar had higher dry mass, phenol, and carotenoid concentrations.
Gene Expression Profiling Reveals New Pathways and Genes Associated with Visna/Maedi Viral Disease
Authors: Abendaño Naiara , Esparza-Baquer Aitor , Bernales Irantzu , Reina Ramsés , de Andrés Damián , Jugo Begoña M.
Published: June 2021

Visna/Maedi virus (VMV) is a lentivirus that infects the cells of the monocyte/macrophage lineage in sheep, goats and wild ruminants. Infection with VMV causes a multisystemic inflammatory disorder, which includes pneumonia, encephalitis, mastitis or arthritis. The immune response to VMV infection is complex, and the infection and pathogenesis of this virus are not totally characterized yet. In this work, a gene expression microarray was used to identify the differentially expressed genes in VMV infection and disease development by comparing sheep with different serologic status and with presence of VM-characteristic clinical lesions. The expression profile analysis has revealed many interesting genes that may be associated with the viral infection process. Among them, the OXT gene appeared significantly up-regulated, so the oxytocin-secreting system could play an essential role in VM disease. Moreover, some of the most significantly enriched functions in up-regulated genes appeared the complement pathway, which (in combination with the Toll-like receptor signaling network) could compose a mechanism in the VMV pathogenesis. Identifying the host genetic factors associated with VMV infection can be applied to develop strategies for preventing infection and develop effective vaccines that lead to therapeutic treatments.
Olive Paste-Enriched Cookies Exert Increased Antioxidant Activities
Authors: Argyri Eleni-Anna , Piromalis Stylianos-Panagiotis , Koutelidakis Antonios , Kafetzopoulos Dimitrios , Petsas Andreas S. , Skalkos Dimitrios , Nasopoulou Constantina , Dimou Charalampia , Karantonis Haralabos C.
Published: June 2021

Functional foods are beneficial to human health and are part of the daily diet of people trying to follow a healthier lifestyle. Olive paste is a good source of functional compounds, mainly phenolic compounds, that have been shown to have health benefits. At the same time, cookies are an ideal snack that can be fortified with additional ingredients to address human dietary needs. The study aimed to enrich cookies with olive paste and extra ingredients for flavor differentiation and evaluate the impact of the enrichment on their antioxidant properties. Enriched cookies were prepared analyzed and tested for sensorial acceptability, total phenolics, and antioxidant activities by DPPH, ABTS, FRAP, and CUPRAC assays. Enriched cookies were sensorially acceptable. Unsaturated fat, total phenolics, and antioxidant activities of enriched cookies were higher compared to control cookies, while among enriched cookies the extra addition of 1% garlic, 0.5% thyme, and 0.5% oregano resulted in higher total phenolics and antioxidant activities compared to cookies that were flavored either with 3% vegetables or 3% orange zest. Antioxidant activity in cookies was strongly correlated with total phenolic content. Cookies enriched with olive paste may be healthy functional food in terms of increased antioxidant activity.
Do Heavy Vehicles Always Have a Negative Effect on Traffic Flow?
Authors: Roh Chang-Gyun , Jeon Hyeonmyeong , Son Bongsoo
Published: June 2021

The purpose of this study is to analyze the effect of heavy vehicles on traffic flow on a two-lane highway. To achieve this goal, data was obtained from piezosensors on the Seoul–Chuncheon Expressway. Analysis of the data showed that, as everyone knows, the average speed of traffic flows decreases as the proportion of heavy vehicles increases. However, not only the speed decreased, but the speed deviation between vehicles decreased. In other words, it was found that within the traffic group that formed the same platoon, individual vehicles were forced to form similar speeds, resulting in a homogeneous rate. This means that heavy vehicles can be included in the traffic stream, reducing the chances of a vehicle-to-vehicle conflict. This kind of influence can be said to explain that heavy vehicles do not necessarily have a negative effect on traffic flow. In this way, we expect to be able to study ways to manage traffic flow by using the effects of low-speed vehicles.
s2p: Provenance Research for Stream Processing System
Authors: Ye Qian , Lu Minyan
Published: June 2021

The main purpose of our provenance research for DSP (distributed stream processing) systems is to analyze abnormal results. Provenance for these systems is not nontrivial because of the ephemerality of stream data and instant data processing mode in modern DSP systems. Challenges include but are not limited to an optimization solution for avoiding excessive runtime overhead, reducing provenance-related data storage, and providing it in an easy-to-use fashion. Without any prior knowledge about which kinds of data may finally lead to the abnormal, we have to track all transformations in detail, which potentially causes hard system burden. This paper proposes s2p (Stream Process Provenance), which mainly consists of online provenance and offline provenance, to provide fine- and coarse-grained provenance in different precision. We base our design of s2p on the fact that, for a mature online DSP system, the abnormal results are rare, and the results that require a detailed analysis are even rarer. We also consider state transition in our provenance explanation. We implement s2p on Apache Flink named as s2p-flink and conduct three experiments to evaluate its scalability, efficiency, and overhead from end-to-end cost, throughput, and space overhead. Our evaluation shows that s2p-flink incurs a 13% to 32% cost overhead, 11% to 24% decline in throughput, and few additional space costs in the online provenance phase. Experiments also demonstrates the s2p-flink can scale well. A case study is presented to demonstrate the feasibility of the whole s2p solution.
MRI and CT Fusion in Stereotactic Electroencephalography: A Literature Review
Authors: Perez Jaime , Mazo Claudia , Trujillo Maria , Herrera Alejandro
Published: June 2021

Epilepsy is a common neurological disease characterized by spontaneous recurrent seizures. Resection of the epileptogenic tissue may be needed in approximately 25% of all cases due to ineffective treatment with anti-epileptic drugs. The surgical intervention depends on the correct detection of epileptogenic zones. The detection relies on invasive diagnostic techniques such as Stereotactic Electroencephalography (SEEG), which uses multi-modal fusion to aid localizing electrodes, using pre-surgical magnetic resonance and intra-surgical computer tomography as the input images. Moreover, it is essential to know how to measure the performance of fusion methods in the presence of external objects, such as electrodes. In this paper, a literature review is presented, applying the methodology proposed by Kitchenham to determine the main techniques of multi-modal brain image fusion, the most relevant performance metrics, and the main fusion tools. The search was conducted using the databases and search engines of Scopus, IEEE, PubMed, Springer, and Google Scholar, resulting in 15 primary source articles. The literature review found that rigid registration was the most used technique when electrode localization in SEEG is required, which was the proposed method in nine of the found articles. However, there is a lack of standard validation metrics, which makes the performance measurement difficult when external objects are presented, caused primarily by the absence of a gold-standard dataset for comparison.
Sliding Mode Control with Sliding Perturbation Observer-Based Strategy for Reducing Scratch Formation in Hot Rolling Process
Authors: Kim Hyun-Hee , Kim Sung-Jin , Yoon Sung-Min , Choi Yong-Joon , Lee Min-Cheol
Published: June 2021

In a hot rolling process, excessive friction between rollers and steel plates may lead to the formation of scratches on the steel plate. To reduce scratch formation in the finishing mill of the hot rolling process, two techniques are proposed in this work: flying touch and velocity synchronization. The proposed flying touch method can reduce the impact of the generated force when the upper roller collides with the steel plate. In addition, the proposed velocity synchronization method can decrease the frictional force resulting from the velocity difference between the rollers and steel plate. The effectiveness of the proposed methods was demonstrated through simulations and experiments using a 1/40 downscaled hot rolling simulator. The simulations and experimental results demonstrate that the proposed methods can reduce the magnitudes of friction and impact forces that lead to scratch formation on the steel plates in the hot rolling process.
Potential of Photovoltaic Generation in the Putumayo Department of Colombia
Authors: Luna Carlosama Carlos Fernando , Moreno Chuquen Ricardo , Mulcue Nieto Luis Fernando , Jiménez García Francy Nelly
Published: June 2021

The potential for generating electricity with photovoltaic systems is high in Colombia given its geographical position in the tropic. Some departments in Colombia have low electricity coverage and high rates. In the department of Putumayo there is a low coverage rate and high energy costs, while the solar radiation potential is high. Due to the geographical differences of the Putumayo subregions, the radiation potential for electricity generation is unknown. In addition, in this department the energy tariffs are above the national average. The objective of this paper is to determine the effective potential for solar photovoltaic power generation in the Putumayo department with a detailed methodology considering the information of different remote database and meteorological stations and some technical conditions. It was found that the highest effective solar potential occurs in the Amazon region, and the lowest in the Andean region in the Putumayo. On the other hand, when evaluating electricity consumption and tariffs in the regions, it is concluded that consumption can be satisfied with photovoltaic systems by producing self-generating electricity and distributed generation.
A Systematic Review of Current Strategies and Methods for BIM Implementation in the Academic Field
Authors: Besné Alia , Pérez Miguel Ángel , Necchi Silvia , Peña Enric , Fonseca David , Navarro Isidro , Redondo Ernesto
Published: June 2021

Since the international governmental institutions required and/or recommended (according to the regulations of each country and continent) all public works to be certified in the BIM (Building Information Modeling) methodology, public and private institutions and universities have sought to integrate BIM into their production and educational processes. This requires the university academic environment to focus its efforts on training students in this methodology, as they will need to apply it in any future work activities related to architecture and construction. This article seeks to analyze which methods are being used by higher education institutions around the world to integrate BIM implementation in AEC (architecture, engineering, and construction) degrees and to determine if a set of regulatory guidelines exists that could serve as a common foundation for institutions to improve this integration process. To this end, a systematic literature review was carried out on WOS and SCOPUS by applying the PRISMA statement methodology. Inclusion and exclusion criteria were selected based on keywords, abstracts, and full content of the articles. In the end, 23 articles were thoroughly studied, the integration and evaluation methods analyzed, and results obtained. The analysis shows a consensus on the need to develop common academic guidelines across university centers that define a strategy for curriculum modifications and teaching and learning strategies. Finally, future lines of research are identified.
Perception and Action under Different Stimulus Presentations: A Review of Eye-Tracking Studies with an Extended View on Possibilities of Virtual Reality
Authors: Heilmann Florian , Witte Kerstin
Published: June 2021

Visual anticipation is essential for performance in sports. This review provides information on the differences between stimulus presentations and motor responses in eye-tracking studies and considers virtual reality (VR), a new possibility to present stimuli. A systematic literature search on PubMed, ScienceDirect, IEEE Xplore, and SURF was conducted. The number of studies examining the influence of stimulus presentation (in situ, video) is deficient but still sufficient to describe differences in gaze behavior. The seven reviewed studies indicate that stimulus presentations can cause differences in gaze behavior. Further research should focus on displaying game situations via VR. The advantages of a scientific approach using VR are experimental control and repeatability. In addition, game situations could be standardized and movement responses could be included in the analysis.
Industry 4.0 HUB: A Collaborative Knowledge Transfer Platform for Small and Medium-Sized Enterprises
Authors: Cotrino Alberto , Sebastián Miguel A. , González-Gaya Cristina
Published: June 2021

Industry 4.0 brings opportunities for small- and medium-sized enterprises (SMEs), but SMEs are lacking Industry 4.0 knowledge, and this might result in a challenge to support SMEs’ competitiveness and productivity. During recent years, the European Commission and some government initiatives have been fostering the transition toward Industry 4.0 for SMEs through the creation of Digital Innovation Hubs, the Plattform Industrie 4.0, and some other initiatives. Nonetheless, the authors consider that the lack of knowledge is still a risk toward Industry 4.0 transformation for SMEs. New ways to improve Industry 4.0 knowledge management and especially the knowledge transfer must be developed. When SMEs start the transition to Industry 4.0, first of all, they do not want to start from scratch, and secondly, it can be easy to get lost in the multitude of technologies and tools that are available in today’s market. There is a gap in which to provide a collaborative Industry 4.0 knowledge transfer platform or hub designed for SMEs. Therefore, this research aims to enhance Industry 4.0 knowledge transfer through the development of a collaborative, web-based knowledge transfer Industry 4.0 platform. The outcome of this research is a developed platform that will be referred to as Industry 4.0 HUB.
Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro
Authors: Wang You , Ma Siyuan , Zou Hongqun , Wu Zhenyu , Luo Zhiyuan , Li Guang
Published: June 2021

Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diameter). The conductive polymer was covered with a Ch selective membrane containing protoporphyrin IX as an ionophore, which exhibited a lower detection limit of 0.49 μM with the potentiometric method. The Ch sensor performed a wide linear range from 1 μM to 1 mM, a fast response time of less than 5 s, and a decent selectivity of common inorganic and organic ions in the human body. Characteristics such as pH and temperature stability, life span, reproducibility and repeatability were also investigated to be satisfied. With the background of artificial cerebrospinal fluid, the recovery rate in 10−5 M of Ch solution was measured by the standard addition method, revealing the potential for biological application.
Texture Evolution and Control of 2524 Aluminum Alloy and Its Effect on Fatigue Crack Propagation Behavior
Authors: Chen Yuqiang , Xiong Chuang , Liu Wenhui , Pan Suping , Song Yufeng , Liu Yang , Zhu Biwu
Published: June 2021

The influences of cold rolling and subsequent heat treatment on the microstructure evolution of 2524 alloy were investigated using an orientation distribution function (ODF) and electron back-scattered diffraction (EBSD). A preparation method of 2524-T3 aluminum alloy with a strong Brass texture was developed, and its effect on the fatigue properties of the alloy was investigated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results show that with the increase in cold rolling deformation from 0% to 80%, the volume fractions of Brass, copper, and S textures in the 2524-T3 alloy also increase, especially in the case of Brass and S textures. However, the volume fractions of cube and Goss textures are reduced significantly, especially for cube textures, which are decreased by 57.4%. Reducing coarse second-phase particles (CSPs) is conducive to the formation of a strong deformation texture during cold rolling. A 10% deformation at each rolling pass, followed by a step annealing, helps the preservation of a Brass texture even after solution treatment at 500 °C for 0.5 h, while a large cold deformation followed by high-temperature annealing helps the formation of a strong cube texture. The Brass texture can enhance the strength while decreasing the fatigue crack growth resistance of this alloy.
On the Generation of Harmonics by the Non-Linear Buckling of an Elastic Beam
Authors: Campos Luiz M. B. C. , Silva Manuel J. S.
Published: June 2021

The Euler–Bernoulli theory of beams is usually presented in two forms: (i) in the linear case of a small slope using Cartesian coordinates along and normal to the straight undeflected position; and (ii) in the non-linear case of a large slope using curvilinear coordinates along the deflected position, namely, the arc length and angle of inclination. The present paper starts with the exact equation in a third form, that is, (iii) using Cartesian coordinates along and normal to the undeflected position like (i), but allowing exactly the non-linear effects of a large slope like (ii). This third form of the equation of the elastica shows that the exact non-linear shape is a superposition of linear harmonics; thus, the non-linear effects of a large slope are equivalent to the generation of harmonics of a linear solution for a small slope. In conclusion, it is shown that: (i) the critical buckling load is the same in the linear and non-linear cases because it is determined by the fundamental mode; (ii) the buckled shape of the elastica is different in the linear and non-linear cases because non-linearity adds harmonics to the fundamental mode. The non-linear shape of the elastica, for cases when powers of the slope cannot be neglected, is illustrated for the first four buckling modes of cantilever, pinned, and clamped beams with different lengths and amplitudes.
Impact of Rain Precipitation on Urban Atmospheric Particle Matter Measured at Three Locations in France between 2013 and 2019
Authors: McMullen Neal , Annesi-Maesano Isabella , Renard Jean-Baptiste
Published: June 2021

As atmospheric particle matter (PM) pollution has been proven to be a public health risk, we investigated how PM concentrations of various particle diameters may be impacted by precipitation. Repeated measures over time of urban PM concentrations for particles of 0.2–50 µm in diameter were compared with precipitation data from Météo-France weather stations in Paris, Angers and Palaiseau from 2013 to 2019. A significant negative correlation, using Kendall’s rank correlation, was found between the amount of precipitation and concentrations of particles >3 µm. Distribution comparative analysis (Dunn’s test) of 154 events of 1 mm or more of rain demonstrated a decrease in concentrations for particles from 10 to 50 µm in diameter. Additionally, granulometric analysis of a typical heavy rain event showed a 10-fold decrease in concentrations of particles 10 to 30 µm in diameter one hour after rain compared with one hour before. We were able to show that measured concentrations of particles between 10 and 50 µm in diameter diminish when it rains, with a lasting effect of approximately 10–15 h.
Hearing Screening among First-Grade Children in Rural Areas and Small Towns in Małopolskie Voivodeship, Poland
Authors: Swierniak Weronika , Skarzynski Piotr , Gos Elzbieta , Czajka Natalia , Matusiak Monika , Hartwich Patryk , Skarzynska Magdalena
Published: June 2021

Undiagnosed hearing deficits hamper a child’s ability to learn. Hearing screening in school aged children helps detect educationally significant hearing loss and prevents negative impacts on academic achievement. The main purpose of this study was to improve early detection and assess the incidence of hearing disorders in first-graders from rural areas and small towns in the Małopolskie Voivodeship of Poland. There were 5029 children aged 6–7 years. Hearing thresholds were measured over the frequency range 0.5–8 kHz. A result was considered positive (abnormal) if the hearing threshold was worse than 20 dB HL at one or more frequencies. The prevalence of hearing loss was estimated in terms of four-frequency hearing loss, high-frequency hearing loss, and low-frequency hearing loss. Parents filled in a brief audiological questionnaire. The analysis was performed using IBM SPSS Statistics, version 24. Of all the children, 20.5% returned a positive result and were referred for further audiological diagnoses. The estimated prevalence of hearing loss was 11.6%, made up of 6.5% with FFHL, 7.6% with HFHL, and 8.2% with LFHL. This study showed that large numbers of children in the district had hearing problems. Adoption of hearing screening in primary schools is recommended as a routine procedure within preventive pediatric health care.
Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects
Authors: Koul Bhupendra , Poonia Anil Kumar , Yadav Dhananjay , Jin Jun-O
Published: June 2021

Nanotechnology is the science of nano-sized particles/structures (~100 nm) having a high surface-to-volume ratio that can modulate the physical, chemical and biological properties of the chemical compositions. In last few decades, nanoscience has attracted the attention of the scientific community worldwide due to its potential uses in the pharmacy, medical diagnostics and disease treatment, energy, electronics, agriculture, chemical and space industries. The properties of nanoparticles (NPs) are size and shape dependent. These characteristic features of nanoparticles can be explored for various other applications such as computer transistors, chemical sensors, electrometers, memory schemes, reusable catalysts, biosensing, antimicrobial activity, nanocomposites, medical imaging, tumor detection and drug delivery. Therefore, synthesizing nanoparticles of desired size, structure, monodispersity and morphology is crucial for the aforementioned applications. Recent advancements in nanotechnology aim at the synthesis of nanoparticles/materials using reliable, innoxious and novel ecofriendly techniques. In contrast to the traditional methods, the biosynthesis of nanoparticles of a desired nature and structure using the microbial machinery is not only quicker and safer but more environmentally friendly. Various microbes, including bacteria, actinobacteria, fungi, yeast, microalgae and viruses, have recently been explored for the synthesis of metal, metal oxide and other important NPs through intracellular and extracellular processes. Some bacteria and microalgae possess specific potential to fabricate distinctive nanomaterials such as exopolysaccharides, nanocellulose, nanoplates and nanowires. Moreover, their ability to synthesize nanoparticles can be enhanced using genetic engineering approaches. Thus, the use of microorganisms for synthesis of nanoparticles is unique and has a promising future. The present review provides explicit information on different strategies for the synthesis of nanoparticles using microbial cells; their applications in bioremediation, agriculture, medicine and diagnostics; and their future prospects.
Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats
Authors: Masodsai Kunanya , Lin Yi-Yuan , Lin Sih-Yin , Su Chia-Ting , Lee Shin-Da , Yang Ai-Lun
Published: June 2021

This study aimed to investigate the aging-related endothelial dysfunction mediated by insulin and insulin-like growth factor-1 (IGF-1) and antioxidant deficiency in hypertension. Male spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar–Kyoto rats (WKYs) were randomly divided into 24-week-old (younger) and 48-week-old (older) groups, respectively. The endothelial function was evaluated by the insulin- and IGF-1-mediated vasorelaxation of aortic rings via the organ bath system. Serum levels of nitric oxide (NO), malondialdehyde (MDA), catalase, and total antioxidant capacity (TAC) were examined. The insulin- and IGF-1-mediated vasorelaxation was significantly impaired in both 24- and 48-week-old SHRs compared with age-matched WKYs and was significantly worse in the 48-week-old SHR than the 24-week-old SHR. After pretreatments of phosphoinositide 3-kinase (PI3K) or NO synthase (NOS) inhibitors, the insulin- and IGF-1-mediated vasorelaxation became similar among four groups. The serum level of MDA was significantly increased, while the NO, catalase, and TAC were significantly reduced in the 48-week-old SHR compared with the 24-week-old SHR. This study demonstrated that the process of aging additively affected insulin- and IGF-1-mediated endothelial dysfunction in SHRs, which could be partly attributed to the reduced NO production and antioxidant deficiency.
PFOS Inhibited Normal Functional Development of Placenta Cells via PPARγ Signaling
Authors: Li Jing , Quan Xiaojie , Lei Saifei , Huang Zhenyao , Wang Qi , Xu Pengfei
Published: June 2021

Perfluorooctane sulfonic acid (PFOS), a persistent environmental pollutant, has adverse effects on gestation pregnancy. Peroxisome proliferator-activated receptor γ (PPARγ) is involved in angiogenesis, metabolic processes, anti-inflammatory, and reproductive development. However, the function of PPARγ in PFOS evoked disadvantageous effects on the placenta remain uncertain. Here, we explored the role of PPARγ in PFOS-induced placental toxicity. Cell viability, cell migration, angiogenesis, and mRNA expression were monitored by CCK-8 assay, wound healing assay, tube formation assay, and real-time PCR, respectively. Activation and overexpression of PPARγ were conducted by rosiglitazone or pcDNA-PPARγ, and inhibition and knockdown of PPARγ were performed by GW9662 or si-PPARγ. Results revealed that PFOS decreased cell growth, migration, angiogenesis, and increased inflammation in human HTR-8/SVneo and JEG-3 cells. Placenta diameter and fetal weight decreased in mice treated with PFOS (12.5 mg/kg). In addition, rosiglitazone or pcDNA-PPARγ rescued cell proliferation, migration, angiogenesis, and decreased inflammation induced by PFOS in HTR8/SVneo and JEG-3 cells. Furthermore, GW9662 or si-PPARγ exacerbated the inhibition of cell viability, migration, angiogenesis, and aggravated inflammation induced by PFOS in HTR-8/SVneo and JEG-3 cells. Meanwhile, the results of mRNA expression level were consistent with the cell representation. In conclusion, our findings revealed that PFOS induced placenta cell toxicity and functional damage through PPARγ pathway.
Improving Digital Patient Care: Lessons Learned from Patient-Reported and Expert-Reported Experience Measures for the Clinical Practice of Multidimensional Walking Assessment
Authors: Scholz Maria , Haase Rocco , Trentzsch Katrin , Stölzer-Hutsch Heidi , Ziemssen Tjalf
Published: June 2021

Background: Walking assessment (WA) enables meaningful patient mobility assessment. In this context, patient satisfaction with WA can influence assessment compliance and indirectly affect outcomes. One opportunity to assess patient satisfaction is patient-reported and expert-reported experience measures (PREM). Research on PREMs and WA in daily clinical multiple sclerosis (MS) practice does not exist yet. Methods: We surveyed people with MS about their experience and assessed healthcare professionals’ experience via an interview after patients completed WA. Results: Gait parameters were related to perceived difficulty and strain during performance. Less impaired patients perceived the WA to be less difficult and exhausting but were less likely to use WA results for themselves. Men and patients with higher impairment would perform WA more frequently. A good workflow, a fully performed WA with standardized testing, fully functional measurement systems, support and safeguarding by staff in case of falls, direct feedback after the testing, and patients’ motivation are identified by the experts as necessary factors for a successful WA. Conclusions: As patients’ experience has an impact on patients’ outcomes, long-term monitoring of PREMs should become an integral part of the healthcare service to identify and avoid problems early.
EV Cargo Sorting in Therapeutic Development for Cardiovascular Disease
Authors: Sherman Cherrie D. , Lodha Shweta , Sahoo Susmita
Published: June 2021

Cardiovascular disease remains the leading cause of morbidity and mortality in the world. Thus, therapeutic interventions to circumvent this growing burden are of utmost importance. Extracellular vesicles (EVs) actively secreted by most living cells, play a key role in paracrine and endocrine intercellular communication via exchange of biological molecules. As the content of secreted EVs reflect the physiology and pathology of the cell of their origin, EVs play a significant role in cellular homeostasis, disease pathogenesis and diagnostics. Moreover, EVs are gaining popularity in clinics as therapeutic and drug delivery vehicles, transferring bioactive molecules such as proteins, genes, miRNAs and other therapeutic agents to target cells to treat diseases and deter disease progression. Despite our limited but growing knowledge of EV biology, it is imperative to understand the complex mechanisms of EV cargo sorting in pursuit of designing next generation EV-based therapeutic delivery systems. In this review, we highlight the mechanisms of EV cargo sorting and methods of EV bioengineering and discuss engineered EVs as a potential therapeutic delivery system to treat cardiovascular disease.
C-REV Retains High Infectivity Regardless of the Expression Levels of cGAS and STING in Cultured Pancreatic Cancer Cells
Authors: Morimoto Daishi , Matsumura Shigeru , Bustos-Villalobos Itzel , Sibal Patricia Angela , Ichinose Toru , Naoe Yoshinori , Eissa Ibrahim Ragab , Abdelmoneim Mohamed , Mukoyama Nobuaki , Miyajima Noriyuki , Tanaka Maki , Kodera Yasuhiro , Kasuya Hideki
Published: June 2021

Oncolytic virus (OV) therapy is widely considered as a major breakthrough in anti-cancer treatments. In our previous study, the efficacy and safety of using C-REV for anti-cancer therapy in patients during stage I clinical trial was reported. The stimulator of interferon genes (STING)–TBK1–IRF3–IFN pathway is known to act as the central cellular host defense against viral infection. Recent reports have linked low expression levels of cGAS and STING in cancer cells to poor prognosis among patients. Moreover, downregulation of cGAS and STING has been linked to higher susceptibility to OV infection among several cancer cell lines. In this paper, we show that there is little correlation between levels of cGAS/STING expression and susceptibility to C-REV among human pancreatic cancer cell lines. Despite having a responsive STING pathway, BxPC-3 cells are highly susceptible to C-REV infection. Upon pre-activation of the STING pathway, BxPc-3 cells exhibited resistance to C-REV infection. However, without pre-activation, C-REV completely suppressed the STING pathway in BxPC-3 cells. Additionally, despite harboring defects in the STING pathway, other high-grade cancer cell lines, such as Capan-2, PANC-1 and MiaPaCa-2, still exhibited low susceptibility to C-REV infection. Furthermore, overexpression of STING in MiaPaCa-2 cells altered susceptibility to a limited extent. Taken together, our data suggest that the cGAS–STING pathway plays a minor role in the susceptibility of pancreatic cancer cell lines to C-REV infection.
Direct Amidation to Access 3-Amido-1,8-Naphthalimides Including Fluorescent Scriptaid Analogues as HDAC Inhibitors
Authors: Hearn Kyle N. , Ashton Trent D. , Acharya Rameshwor , Feng Zikai , Gueven Nuri , Pfeffer Frederick M.
Published: June 2021

Methodology to access fluorescent 3-amido-1,8-naphthalimides using direct Buchwald–Hartwig amidation is described. The protocol was successfully used to couple a number of substrates (including an alkylamide, an arylamide, a lactam and a carbamate) to 3-bromo-1,8-naphthalimide in good yield. To further exemplify the approach, a set of scriptaid analogues with amide substituents at the 3-position were prepared. The new compounds were more potent than scriptaid at a number of histone deacetylase (HDAC) isoforms including HDAC6. Activity was further confirmed in a whole cell tubulin deacetylation assay where the inhibitors were more active than the established HDAC6 selective inhibitor Tubastatin. The optical properties of these new, highly active, compounds make them amenable to cellular imaging studies and theranostic applications.