bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of Primary Hypertension and Risk Factors in Grade XII Learners in KwaZulu-Natal, South Africa
Authors: Bhimma Rajendra , Naicker Elaene , Gounden Verena , Nandlal Louansha , Connolly Cathy , Hariparshad Sudesh
Published: July 2018

Hypertension in childhood leads to hypertension in adult life, the strongest risk factor being obesity. This study determined the prevalence of primary hypertension and its risk factors in Grade XII learners in KwaZulu-Natal, South Africa, from March 2016 to June 2017. Weight, height, body mass index (BMI), random finger prick cholesterol and glucose, and spot urine for an albumin : creatinine ratio were measured. An average of three separate blood pressure readings taken was at least 5 minutes apart. Five hundred and sixty-four learners had weight, height, and BMI; 536 had random blood glucose; and 545 had cholesterol and random spot urine albumin : creatinine ratios measured. Prehypertension was detected in 168 (29.7%) and hypertension in 77 (13.7%) of learners. Ninety (15.9%) were overweight and 75 (13,3%) were obese. Hypercholesterolaemia was present in 58 (10.8%) and a high spot random urine albumin : creatinine ratio in 5 (1.0%). None had a high blood glucose level. Both prehypertension and hypertension in all learners showed a significant increase with increasing BMI. Six (1.0%) learners had metabolic syndrome. Female learners in other racial groups (defined as Indian, mixed race, and White learners), overweight, and obese learners showed significantly higher rates of hypercholesterolaemia. We showed overweight and obesity as risk factors for prehypertension and hypertension. This presages the need for an appropriate diet and adequate exercise in a child’s school career.
Blackberry and Blueberry Anthocyanin Supplementation Counteract High‐Fat‐Diet‐Induced Obesity by Alleviating Oxidative Stress and Inflammation and Accelerating Energy Expenditure
Authors: Wu Tao , Gao Yufang , Guo Xueqi , Zhang Min , Gong Lingxiao
Published: July 2018

Many studies indicate that an anthocyanin‐rich diet has beneficial effects preventing metabolic disease. In the present study, the molecular mechanism underlying the antiobesity effect of consuming blackberry anthocyanins (BLA) and blueberry anthocyanins (BBA) was investigated in high‐fat‐diet‐ (HFD‐) fed C57BL/6 mice. Sixty mice were administered a low‐fat diet (LFD), a HFD, or a HFD plus orlistat, and BLA or BBA in their daily food for 12 weeks. As a result, the consumption of BLA and BBA inhibited body weight gain by 40.5% and 55.4%, respectively, in HFD‐fed mice. The BLA and BBA treatments markedly reduced serum and hepatic lipid levels and significantly increased hepatic superoxide dismutase and glutathione peroxidase activities. In addition, the treatments effectively increased fecal acetate and butyrate levels and significantly attenuated expression of tumor necrosis factor TNF‐α, interleukin‐6, and nuclear factor‐kappaB genes. Moreover, gas chromatography time‐of‐flight mass spectroscopy results suggested that BLA and BBA significantly affected the hepatic lipid and glucose metabolic pathways, including glycerophospholipid metabolism, glutathione metabolism, and the insulin‐signaling pathway. Therefore, BLA and BBA ameliorated diet‐induced obesity by alleviating oxidative stress and inflammation and accelerating energy expenditure.
Effect of a Combination of Prednisone or Mycophenolate Mofetil and Mesenchymal Stem Cells on Lupus Symptoms in MRL.Faslpr Mice
Authors: Lee Hong Kyung , Kim Ki Hun , Kim Hyung Sook , Kim Ji Sung , Lee Jae Hee , Ji Ayoung , Kim Kyung Suk , Lee Tae Yong , Chang In Young , Bae Sang-Cheol , Hong Jin Tae , Kim Youngsoo , Han Sang-Bae
Published: July 2018

The combination of immunosuppressants and mesenchymal stem cells (MSCs) is a promising therapeutic strategy for systemic lupus erythematosus, since this approach reduces doses of immunosuppressants while maintaining the same therapeutic outcome. However, it is unavoidable for MSCs to be exposed to immunosuppressants. Here, we examined the combination effect of prednisone (PD) or mycophenolate mofetil (MMF) and MSCs. We showed that PD or MMF in combination with MSCs showed better therapeutic effect than single therapy in lupus-prone MRL.Faslpr mice, as assessed by using the following readouts: prolongation of survival, decrease in anti-dsDNA and total IgG levels in serum, decrease in cytokine gene expression in spleen cells, and decrease in inflammatory cell infiltration into the kidney. In vitro, immunosuppressants and MSCs inhibited T cell proliferation in a synergistic manner. However, immunosuppressants did not affect MSC viability and functions such as TGF-β1 and PGE2 production, migration, and immunosuppressive capacity. In summary, our study demonstrates that a combination of immunosuppressants and MSCs is a good strategy to reduce the side effects of PD and MMF without the loss of therapeutic outcome.
A Combined Localization Algorithm for Wireless Sensor Networks
Authors: Qi Xiaogang , Liu Xiaoke , Liu Lifang
Published: July 2018

Wireless sensor networks (WSNs) are widely used in various fields to monitor and track various targets by gathering information, such as vehicle tracking and environment and health monitoring. The information gathered by the sensor nodes becomes meaningful only if it is known where it was collected from. Considering that multilateral algorithm and MDS algorithm can locate the position of each node, we proposed a localization algorithm combining the merits of these two approaches, which is called MA-MDS, to reduce the accumulation of errors in the process of multilateral positioning algorithm and improve the nodes’ positioning accuracy in WSNs. It works in more robust fashion for noise sparse networks, even with less number of anchor nodes. In the MDS positioning phase of this algorithm, the Prussian Analysis algorithm is used to obtain more accurate coordinate transformation. Through extensive simulations and the repeatable experiments under diverse representative networks, it can be confirmed that the proposed algorithm is more accurate and more efficient than the state-of-the-art algorithms.
Osthole Protects against Acute Lung Injury by Suppressing NF-κB-Dependent Inflammation
Authors: Jin Yiyi , Qian Jianchang , Ju Xin , Bao Xiaodong , Li Li , Zheng Suqing , Chen Xiong , Xiao Zhongxiang , Chen Xuemei , Zhu Weiwei , Li Weixin , Wu Wencan , Liang Guang
Published: July 2018

Inflammation is a key factor in the pathogenesis of ALI. Therefore, suppression of inflammatory response could be a potential strategy to treat LPS-induced lung injury. Osthole, a natural coumarin extract, has been reported to protect against acute kidney injury through an anti-inflammatory mechanism, but its effect on ALI is poorly understood. In this study, we investigated whether osthole ameliorates inflammatory sepsis-related ALI. Results from in vitro studies indicated that osthole treatment inhibited the LPS-induced inflammatory response in mouse peritoneal macrophages through blocking the nuclear translocation of NF-κB. Consistently, the in vivo studies indicated that osthole significantly prolonged the survival of septic mice which was accompanied by inflammation suppression. In the ALI mouse model, osthole effectively inhibited the development of lung tissue injury, leukocytic recruitment, and cytokine productions, which was associated with inhibition of NF-κB nuclear translocation. These findings provide evidence that osthole was a potent inhibitor of NF-κB and inflammatory injury and suggest that it could be a promising anti-inflammatory agent for therapy of septic shock and acute lung injury.
Global Output Feedback Stabilization for a Class of Nonlinear Cascade Systems
Authors: Liu Cai-Yun , Sun Zong-Yao , Meng Qing-Hua , Chen Chih-Chiang , Cai Bin , Shao Yu
Published: July 2018

This paper focuses on the problem of global output feedback stabilization for a class of nonlinear cascade systems with time-varying output function. By using double-domination approach, an output feedback controller is developed to guarantee the global asymptotic stability of closed-loop system. The novel control strategy successfully constructs a unified Lyapunov function, which is suitable for both upper-triangular and lower-triangular systems. Finally, two numerical examples are provided to illustrate the effectiveness of a control strategy.
The Effect of Immunosuppressive Drugs on MDSCs in Transplantation
Authors: Yang Fan , Li Yang , Zhang Qian , Tan Liang , Peng Longkai , Zhao Yong
Published: July 2018

Myeloid-derived suppressor cells (MDSCs) are a group of innate immune cells that regulates both innate and adaptive immune responses. In recent years, MDSCs were shown to play an important negative regulatory role in transplant immunology even upstream of regulatory T cells. In certain cases, MDSCs are closely involved in transplantation immune tolerance induction and maintenance. It is known that some immunosuppressant drugs negatively regulate MDSCs but others have positive effects on MDSCs in different transplant cases. We herein summarized our recent insights into the regulatory roles of MDSCs in transplantation specially focusing on the effects of immunosuppressive drugs on MDSCs and their mechanisms of action. Studies on the effects of immunosuppressive drugs on MDSCs will significantly expand our understanding of immunosuppressive drugs on immune regulatory cells in transplantation and offer new insights into transplant tolerance. We hope to emphasize our concern for the negative effects of immunosuppressive agents on MDSCs, which may potentially attenuate the immune tolerance induction in transplanted recipients.
A Novel Wire Is Effective for Echo-Guiding Percutaneous Atrial Septal Defect Closure: A Preclinical Study
Authors: Liu Yao , Guo Gai-Li , Zhang Feng-Wen , Wen Bin , Ou-Yang Wen-Bin , Xie Yong-quan , Pan Xiang-Bin
Published: July 2018

Objective. To assess the effectiveness of a novel ultrasound wire for echo-guiding percutaneous atrial septal defect (ASD) closure in a sheep model. Methods. After right lateral thoracotomy, ASDs were created in 20 sheep by transseptal needle puncture followed by balloon dilatation. Animals were evenly randomized into 2 groups to undergo ASD closure using echography as the only imaging tool with either COOK wire (control group) or new ultrasound wire (study group). The total procedural time, passing time (time needed for the guide wire to enter the left atrium), frequency of delivery sheath dropping into the right atrium, frequency of arrhythmias, and 1-week rate of complications were compared between the two groups. Results. All animals survived defect creation procedures uneventfully. ASD devices were successfully implanted in all sheep. Compared with the control group, the study group had significantly (P<0.05) lower mean procedure time (15.36 ± 4.86 versus 25.82 ± 7.85 min), lower mean passing time (2.69 ± 0.82 versus 5.58 ± 3.34 min), lower frequency of the guide wire dropping into the right atrium (0% versus 40%), and lower frequency of atrial (4.41 ± 2.61 versus 9.60 ± 3.68) or ventricular premature contractions (0.75 ± 0.36 versus 1.34 ± 0.68), respectively, without serious complications up to one week. Conclusion. The novel ultrasound specialized guide wire was effective in echo-guiding percutaneous ASD closure.
Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen
Authors: Duruksu Gokhan , Aciksari Aysegul
Published: July 2018

The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes. In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation. The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose. After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared. The results showed that the modifications did not induce apoptosis or cause cell death. However, the culture of cells on modified collagens improved the proliferation. It was found that the mannose-modified collagen stimulated the adipogenic differentiation of stem cells, and rhamnose-modified collagen supports the differentiation into both osteogenic and insulin-producing cells. The low concentration of monosaccharides during glycation process improved the characteristics of the matrix protein in favor of stem cell differentiation. Modification of the collagen by glycation might be used as a tool to improve natural polymers for material-induced stem cell differentiation in the future.
The Relation between Asthma Control and Quality of Life in Children
Authors: Banjari M. , Kano Y. , Almadani S. , Basakran A. , Al-Hindi M. , Alahmadi T.
Published: July 2018

Background. Asthma is a common chronic illness worldwide. Asthmatic children are forced to alter their way of living to avoid its complications or exacerbations, which negatively affects their psychological and social well-being. High prevalence of behavioral and emotional difficulties was noticed among children with asthma. Methods. Cross-sectional study that was conducted over 8 months involving asthmatic children within the ages of 7-17 years presenting to two governmental hospitals in Jeddah, Saudi Arabia. Three questionnaires were used: asthma control test, the strengths and difficulties questionnaire, and the pediatrics asthma quality of life questionnaire. Using SPSS, Pearson’s chi-square and independent sample t-tests were used to find associations. Results. Among the 106 respondents, 84% of the sample had poor asthma control. Significantly poorer quality of life was observed in children with uncontrolled asthma (p = <0.001). Children with controlled and uncontrolled asthma were equally affected psychosocially with no relation between asthma control and their psychosocial well-being (p = 0.58). Conclusion. The majority of asthmatic children were uncontrolled with poor quality of life. This study recommends that the psychosocial well-being should be assessed during clinic visits for a better holistic approach and effective improvement of outcome. Further researches are needed to study the psychological effect of asthma.
Heuristics and Recommendations for the Design of Mobile Serious Games for Older Adults
Authors: Machado Mônica da Consolação , Ferreira Ronan Loschi Rodrigues , Ishitani Lucila
Published: July 2018

As people get older, their physical and cognitive functions decline. Meeting these needs is an important goal to be reached, to propose activities that may contribute to a better quality of life, including leisure and learning. As such, this paper aims to present a set of heuristics and recommendations for the design of mobile serious games for older adults. To elaborate these heuristics and recommendations, we conducted two studies with older people. In the first study, we invited people aged 60 or more to evaluate mobile games, intending to collect data that could help us to adapt the heuristics that were already published to the public of this research. In the period between the two studies, we developed a serious mobile game (Labuta​ Batuta​) that met the set of adapted heuristics. In the second study, we invited another group of older people, aged 45 or over, to qualitatively evaluate Labuta​ Batuta​, in order to develop a Grounded Theory about the desirable characteristics for the design of serious mobile games for older adults. Besides a Grounded Theory, the second study allowed the identification of nine requirements to be considered when designing the mechanics of mobile games for older adults.
Immune Dysfunction and Coinfection with Human Immunodeficiency Virus andSchistosoma japonicumin Yi People
Authors: Yang Yu , Xiao Peng-Lei , Yang Ya , Gao Jian-Chuan , Shi Yan , Cheng Wan-Ting , Chen Yue , Song Xiu-Xia , Jiang Qing-Wu , Zhou Yi-Biao
Published: July 2018

Objective. To explore the association between infections with HIV andSchistosoma japonicum, and to determine the influences of the HIV-S. japonicumcoinfections on the immune system of Yi people.Methods. A block design study was conducted in a Yi county in southwestern China, one of the endemic areas of both HIV/AIDS andS. japonicumin China. All participants were screened for HIV antibodies andS. japonicumantibodies (SjAb) and were classified into four groups: HIV(+)/S. japonicum(−), HIV(−)/S. japonicum(+), HIV(+)/S. japonicum(+), and HIV(−)/S. japonicum(−).Results. There were significant differences among the four groups in both CD4+T lymphocytes and CD8+T lymphocytes, but no significant difference in CD3+T lymphocytes. Both the CD4+T lymphocyte counts and the ratio of CD4+/CD8+were lower in HIV-infected people compared with those uninfected. People infected withS. japonicumhad increased CD4+T lymphocyte counts but reduced CD8+T lymphocyte counts. Similarly, the ratio of CD4+/CD8+was higher inS. japonicum-infected people compared with those uninfected. People coinfected with HIV andS. japonicumhad lower CD4+T lymphocyte counts, lower ratio of CD4+/CD8+, and higher CD8+T lymphocyte counts compared with those infected with HIV only orS. japonicumonly. People infected with HIV only and those coinfected with HIV andS. japonicumhad a higher level of IFN-γcompared with people with no infection. There were no significant differences between people infected with HIV only and withS. japonicumonly in the levels of IFN-γand IL-10.Conclusions. People coinfected with HIV andS. japonicummight have a suppressed immune function because of a decrease in CD4+T lymphocyte counts, a lowered ratio of CD4+/CD8+, and an increase in CD8+T lymphocyte counts. Coinfection with HIV andS. japonicumwould alter the level of IFN-γin plasma.
Structural, Morphological, Optical, and Room Temperature Magnetic Characterization on Pure and Sm-Doped ZnO Nanoparticles
Authors: Badreddine Khalil , Kazah I. , Rekaby M. , Awad R.
Published: July 2018

Nano crystalline Zn1-xSmxO, (0.00 ≤ x ≤ 0.10), were prepared by wet chemical coprecipitation method. The effect of samarium doping on the structural, morphological, optical, and magnetic properties of ZnO nanoparticles was examined by X-ray powder diffraction (XRD), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), Ultraviolet-visible spectroscopy (UV) and M-H magnetic hysteresis. XRD analysis showed the hexagonal wurtzite structure of ZnO. The absence of Sm2O3as separate phase may be attributed to the complete dissolving of samarium in ZnO lattice. The lattice parameters (aandc) of Zn1-xSmxO were calculated and they fluctuated with the increase of Sm doping which indicated that the structure of ZnO was perturbed by the doping of Sm. The crystallite size was computed for all the samples using Debye-Scherrer’s method. The crystallite size decreased with the increase of Sm doping. TEM micrographs revealed that the size and the shape of the ZnO nanocomposites were changed by modifying the doping level of samarium. FTIR analysis spectrum confirmed the formation of ZnO phase and revealed a peak shift between pure and Sm-doped ZnO. The band gap energy and Urbach energy were calculated for Zn1-xSmxO, (0.00 ≤ x ≤ 0.10). The band energy gaps of pure and Sm doped ZnO samples are in the range 2.6–2.98 eV. M-H hysteresis inspection, at room temperature, showed that the pure ZnO exhibited a ferromagnetic behavior incorporated with diamagnetic and paramagnetic contributions. Ferromagnetic behavior was reduced for the doped samples withx=0.01andx=0.04. The samples withx=0.02and 0.06 ≤ x ≤ 0.10 tend to be superparamagnetic. The saturation magnetization (Ms), the coercivity (Hc), and the retentivity (Mr) were recorded for Zn1-xSmxO, (0.00 ≤ x ≤ 0.10).
Update on the Benefits and Mechanisms of Action of the Bioactive Vegetal Alkaloid Berberine on Lipid Metabolism and Homeostasis
Authors: Wang Yanwen , Zidichouski Jeffrey A.
Published: July 2018

Elevation of circulating levels of blood cholesterol, especially LDL cholesterol, and/or the decrease of HDL cholesterol levels have long been recognized as primary risk factors for developing atherosclerosis that leads to cardiovascular and cerebrovascular disease. Hypertriglyceridemia is an independent risk factor that is known to contribute to the development of atherosclerosis. Thus, various interventional efforts aimed at reducing hypercholesterolemia and hypertriglyceridemia have been practiced clinically for decades to reduce morbidity and mortality risk associated with deleterious cardiovascular and cerebrovascular events. As such, many drugs have been developed and clinically used to treat hypocholesteremia and/or hypertriglyceridemia; however, dietary approaches including supplements along with changes in nutrition and lifestyle have become increasingly attractive and acceptable methods used to control borderline or moderately increased levels of blood cholesterol and triacylglycerols. In this regard, the use of a plant/herbal bioactive compound, berberine (BBR), has recently been studied extensively in terms of its efficacy as well as its mechanisms of action and safety as an alternative intervention that beneficially modulates blood lipids. The aim of this review is to provide a comprehensive update on BBR research, new concepts and directions in terms of product development and current challenges, and future prospects of using BBR to manage diseases and complications associated with dyslipidemia.
Oxidative Stress and Necrotizing Enterocolitis: Pathogenetic Mechanisms, Opportunities for Intervention, and Role of Human Milk
Authors: Aceti Arianna , Beghetti Isadora , Martini Silvia , Faldella Giacomo , Corvaglia Luigi
Published: July 2018

This review will examine the role of oxidative stress (OS) in the pathogenesis of necrotizing enterocolitis (NEC) and explore potential preventive and therapeutic antioxidant strategies. Preterm infants are particularly exposed to OS as a result of several perinatal stimuli and constitutive defective antioxidant defenses. For this reason, OS damage represents a contributing factor to several complications of prematurity, including necrotizing enterocolitis (NEC). Being NEC a multifactorial disease, OS may act as downstream component of the pathogenetic cascade. To counteract OS in preterm infants with NEC, several antioxidant strategies have been proposed and different antioxidant compounds have been experimented. It is well known that human milk (HM) is an important source of antioxidants. At the same time, the role of an exclusive HM diet is well recognized in the prevention of NEC. However, donor HM (DHM) processing may impair antioxidant properties. As DHM is becoming a common nutritional intervention for high risk PI, the antioxidant status of preterm and DHM and potential ways to preserve its antioxidant capacity may merit further investigation.
Slipping Rib Syndrome in a Female Adult with Longstanding Intractable Upper Abdominal Pain
Authors: Khan Noman Ahmed Jang , Waseem Saba , Ullah Saad , Mehmood Hassan
Published: July 2018

Slipping rib syndrome is a rare cause of abdominal or lower chest pain that can remain undiagnosed for many years. Awareness among health care personnel of this rare but significant disorder is necessary for early recognition. Prompt treatment can avoid unnecessary testing, radiographic exposure, and years of debilitating pain. A 52-year-old female was evaluated for a 3-year history of recurrent abdominal and lower chest pain. Pain was sharp, primarily located in the lower chest and subcostal region left more than right, waxing and waning, nonradiating, and aggravates with specific movements. She underwent frequent physical therapies, treated with multiple muscle relaxants and analgesics with minimal improvement. Imaging modalities including CT scan, MRI, and X-rays performed on multiple occasions failed to signify any underlying abnormality. Complete physical examination was unremarkable except for positive hooking maneuver. Dynamic flow ultrasound of lower chest was performed which showed slipping of the lowest rib over the next lowest rib bilaterally left worse than right, findings consistent with slipping rib syndrome. Slipping rib syndrome is caused by hypermobility of the floating ribs (8 to 12) which are not connected to the sternum but attached to each other with ligaments. Diagnosis is mostly clinical, and radiographic tests are rarely necessary. Hooking maneuver is a simple clinical test to reproduce pain and can aid in the diagnosis. Reassurance and avoiding postures that worsen pain are usually helpful. In refractory cases, nerve block and surgical intervention may be required.
Improved Design of Bit Synchronization Clock Extraction in Digital Communication System
Authors: Duan Huimin , Huang Hui , Li Cuihua
Published: July 2018

An improved method is proposed in this design to reduce the phase jitter after the synchronization or the random noise induced phase jitter in a bit synchronization clock extraction circuit. By using a newly added digital filter between the phase detector and the controller, the phase difference pulses from the phase detector are counted and processed, before being transmitted to the controller for adjusting the phase of the output clock. The design is completed by using FPGA chip and VHDL hardware description language and performs the simulation verification on Quartus II. The results show that the improved system performs the accurate extraction of bit synchronized clock, reduces the phase jitter problem, improves the system running efficiency and the ability of anti-interference, and guarantees the synchronization performance of the digital communication system.
Antidepressant Effect ofFraxinus rhynchophyllaHance Extract in a Mouse Model of Chronic Stress-Induced Depression
Authors: Kim Yu Ri , Park Bo-Kyung , Kim Young Hwa , Shim Insop , Kang In-Cheol , Lee Mi Young
Published: July 2018

Prolonged exposure to stress can affect mood and cognition and lead to mood disorders. Research on stress-associated mood disorders is important in modern society as people are increasingly exposed to unavoidable stressors. We used a mouse model with 2 weeks of exposure to electric foot shock and restraint, to determine the effect ofFraxinus rhynchophyllaHance (FX) extract on chronic stress-induced depression. We measured the effect of FX extract using various physiological, behavioral, and biochemical measures. FX extract ameliorated chronic stress-induced body and relative liver weight loss and improved depressive-like behaviors in the open field and forced swim tests. In addition, plasma cortisol and serotonin levels in stress-induced mice following FX treatment were similar to normal mice, and the elevation of proinflammatory cytokines was prevented. Moreover, FX treatment increased the expression of phosphorylated cyclic adenosine-3′,5′-monophosphate response element-binding protein (pCREB)/brain-derived neurotrophic factor (BDNF). Further experiments confirmed the efficacy of FX extract by showing similar results using esculin and esculetin, compounds extracted from FX. Taken together, these results indicate that FX extract has an antidepressant effect on chronic stress-induced depression by associating signaling with neuroinflammation and neurogenesis.
Possible Modulation of Vascular Function Measures in Rheumatoid Arthritis by Homocysteine
Authors: Alomari Mahmoud A. , Khabour Omar F. , Alawneh Khaldoon , Shammaa Rania A.
Published: July 2018

The effect of homocysteine on cardiovascular diseases is still equivocal, especially in rheumatoid arthritis patients. In this investigation, the association between homocysteine with blood flow and vascular resistance in rheumatoid arthritis was examined. Serum levels of homocysteine were determined in thirty-one rheumatoid arthritis patients and nineteen apparently healthy subjects using ELISA. Additionally, strain-gauge plethysmography was used to determine both forearm blood flow and vascular function at rest and after occlusion. Forearm occlusion blood flow (patients: 21.9 ± 6.55 versus control: 25.5 ± 6.10ml/100mL/min) was lower (p < 0.05) while occlusion vascular resistance (patients: 4.77 ± 2.08 versus controls 3.05 ± 0.96U) was greater (p < 0.01) in rheumatoid arthritis than in the controls. Level of serum homocysteine was similar (p = 0.803) in rheumatoid arthritis group and healthy group. In addition, level of serum homocysteine was correlated with resting blood flow (r = −0.41; p < 0.02) and resting vascular resistance (r = 0.31, p < 0.05) in the patients group. The study confirms altered vascular function in rheumatoid arthritis. Uniquely, the results show that homocysteine was related to resting, but not postischemia, vascular measures. These relationships indicate that homocysteine might impact the vasculature in rheumatoid arthritis.
Recent Progress of Imprinted Nanomaterials in Analytical Chemistry
Authors: Keçili Rüstem , Hussain Chaudhery Mustansar
Published: July 2018

Molecularly imprinted polymers (MIPs) are a type of tailor-made materials that have ability to selectively recognize the target compound/s. MIPs have gained significant research interest in solid-phase extraction, catalysis, and sensor applications due to their unique properties such as low cost, robustness, and high selectivity. In addition, MIPs can be prepared as composite nanomaterials using nanoparticles, multiwalled carbon nanotubes (MWCNTs), nanorods, quantum dots (QDs), graphene, and clays. This review paper aims to demonstrate and highlight the recent progress of the applications of imprinted nanocomposite materials in analytical chemistry.
Two-Year Outcomes of Visian Implantable Collamer Lens with a Central Hole for Correcting High Myopia
Authors: Yan Zhipeng , Miao Huamao , Zhao Feng , Wang Xiaoying , Chen Xun , Li Meiyan , Zhou Xingtao
Published: July 2018

Purpose. To investigate two-year outcomes of Visian Implantable Collamer Lens with a central hole (ICL V4c) implantation for correcting high myopia.Methods. Sixty-one eyes of 32 patients went through ICL V4c implantation. Safety, efficacy, predictability, and intraocular pressure were evaluated 2 years postoperatively. Anterior chamber volume (ACV), anterior chamber depth (ACD), anterior chamber angle width (ACAW), and vault were measured using a Scheimpflug tomography imaging system.Results. At 2 years, the spherical equivalent refraction decreased from preoperative −14.62 ± 4.29 D to −0.90 ± 0.95 D, with 79% of the eyes within ±0.50 D and 98% within ±1.00 D of the intended correction. The efficacy index was 1.03 ± 0.23, and the safety index was 1.24 ± 0.26. Corrected distance visual acuity (CDVA) remained unchanged in 23% of the eyes, 75% gained one or more lines of CDVA, and 2% lost one line. The ACV, ACD, and ACAW, respectively, decreased from 193.28 ± 29.15 mm3, 3.15 ± 0.23 mm, and 36.51 ± 6.54 degree to 112.48 ± 17.01 mm3, 2.99 ± 0.23 mm, and 22.54 ± 5.27 degree (p=0.0008,  0.008,  and  0.0003, resp.). Intraocular pressure was 15.39 ± 2.88 mmHg before surgery and was 15.86 ± 4.11 mmHg at 2 years (p=0.11).Conclusion. Implantation of ICL V4c is a safe, effective, and predictable procedure for correcting high myopia. Reduction of anterior chamber space after surgery did not induce intraocular pressure increase during the 2-year follow-up.
Tumor Location Correlates with Clinicopathological Features and Prognosis of the Solid Pseudopapillary Neoplasm
Authors: Meng Zibo , Cao Mingsi , Wang Feiyang , Zhang Yushun , Wu Heshui
Published: July 2018

Background. Solid pseudopapillary neoplasms (SPNs) of the pancreas are rare neoplasms with low malignant behavior. These neoplasms can be aggressive and cause bad ending of SPN patients. The purpose of this article is to identify certain prognostic factors.Method. We retrospectively evaluated 196 patients from our hospital and SEER database. We identified that tumor location was an independent prognostic indicator of SPN patients.Results. DSS and OS of pancreatic head SPNs (HOP) were significantly shorter than those of other locations (OOP). Operation methods and age were different between HOP and OOP groups. Compared to OOP group, patients in HOP group were younger. Operation time was longer, and hospital stays were longer.Conclusion. This work suggests that pancreatic head SPNs have distinct clinicopathological features and clinical outcome. It is urgent to optimize the treatment of SPN patients and identify effective prognostic indicators of SPN.
An Evaluation of a Metaheuristic Artificial Immune System for Household Energy Optimization
Authors: Navarro-Caceres Maria , Herath Pramod , Villarrubia Gabriel , Prieto-Castrillo Francisco , Venyagamoorthy G. Kumar
Published: July 2018

Devices in a smart home should be connected in an optimal way; this helps save energy and money. Among numerous optimization models that can be found in the literature, we would like to highlight artificial immune systems, which use special bioinspired algorithms to solve optimization problems effectively. The aim of this work is to present the application of an artificial immune system in the context of different energy optimization problems. Likewise, a case study is performed in which an artificial immune system is incorporated in order to solve an energy management problem in a domestic environment. A thorough analysis of the different strategies is carried out to demonstrate the ability of an artificial immune system to find a successful optima which satisfies the problem constraints.
Paclitaxel Sensitivity of Ovarian Cancer Can be Enhanced by Knocking Down Pairs of Kinases that Regulate MAP4 Phosphorylation and Microtubule Stability
Authors: Yang Hailing , Mao Weiqun , Rodriguez-Aguayo Cristian , Mangala Lingegowda S. , Bartholomeusz Geoffrey , Iles Lakesla R. , Jennings Nicholas B. , Ahmed Ahmed Ashour , Sood Anil K. , Lopez-Berestein Gabriel , Lu Zhen , Bast Robert C.
Published: July 2018

Purpose: Most patients with ovarian cancer receive paclitaxel chemotherapy, but less than half respond. Pre-treatment microtubule stability correlates with paclitaxel response in ovarian cancer cell lines. Microtubule stability can be increased by depletion of individual kinases. As microtubule stability can be regulated by phosphorylation of microtubule-associated proteins (MAPs), we reasoned that depletion of pairs of kinases that regulate phosphorylation of MAPs could induce microtubule stabilization and paclitaxel sensitization.

Experimental Design: Fourteen kinases known to regulate paclitaxel sensitivity were depleted individually in 12 well-characterized ovarian cancer cell lines before measuring proliferation in the presence or absence of paclitaxel. Similar studies were performed by depleting all possible pairs of kinases in six ovarian cancer cell lines. Pairs that enhanced paclitaxel sensitivity across multiple cell lines were studied in depth in cell culture and in two xenograft models.

Results: Transfection of siRNA against 10 of the 14 kinases enhanced paclitaxel sensitivity in at least six of 12 cell lines. Dual knockdown of IKBKB/STK39 or EDN2/TBK1 enhanced paclitaxel sensitivity more than silencing single kinases. Sequential knockdown was superior to concurrent knockdown. Dual silencing of IKBKB/STK39 or EDN2/TBK1 stabilized microtubules by inhibiting phosphorylation of p38 and MAP4, inducing apoptosis and blocking cell cycle more effectively than silencing individual kinases. Knockdown of IKBKB/STK39 or EDN2/TBK1 enhanced paclitaxel sensitivity in two ovarian xenograft models.

Conclusions: Sequential knockdown of dual kinases increased microtubule stability by decreasing p38-mediated phosphorylation of MAP4 and enhanced response to paclitaxel in ovarian cancer cell lines and xenografts, suggesting a strategy to improve primary therapy. Clin Cancer Res; 24(20); 5072–84. ©2018 AACR.
Consensus on Molecular Subtypes of High-Grade Serous Ovarian Carcinoma
Authors: Chen Gregory M. , Kannan Lavanya , Geistlinger Ludwig , Kofia Victor , Safikhani Zhaleh , Gendoo Deena M.A. , Parmigiani Giovanni , Birrer Michael , Haibe-Kains Benjamin , Waldron Levi
Published: July 2018

Purpose: The majority of ovarian carcinomas are of high-grade serous histology, which is associated with poor prognosis. Surgery and chemotherapy are the mainstay of treatment, and molecular characterization is necessary to lead the way to targeted therapeutic options. To this end, various computational methods for gene expression–based subtyping of high-grade serous ovarian carcinoma (HGSOC) have been proposed, but their overlap and robustness remain unknown.

Experimental Design: We assess three major subtype classifiers by meta-analysis of publicly available expression data, and assess statistical criteria of subtype robustness and classifier concordance. We develop a consensus classifier that represents the subtype classifications of tumors based on the consensus of multiple methods, and outputs a confidence score. Using our compendium of expression data, we examine the possibility that a subset of tumors is unclassifiable based on currently proposed subtypes.

Results: HGSOC subtyping classifiers exhibit moderate pairwise concordance across our data compendium (58.9%–70.9%; P < 10−5) and are associated with overall survival in a meta-analysis across datasets (P < 10−5). Current subtypes do not meet statistical criteria for robustness to reclustering across multiple datasets (prediction strength < 0.6). A new subtype classifier is trained on concordantly classified samples to yield a consensus classification of patient tumors that correlates with patient age, survival, tumor purity, and lymphocyte infiltration.

Conclusions: A new consensus ovarian subtype classifier represents the consensus of methods and demonstrates the importance of classification approaches for cancer that do not require all tumors to be assigned to a distinct subtype. Clin Cancer Res; 24(20); 5037–47. ©2018 AACR.
Role of EphB3 Receptor in Mediating Head and Neck Tumor Growth, Cell Migration, and Response to PI3K Inhibitor
Authors: Bhatia Shilpa , Griego Anastacia , Lennon Shelby , Oweida Ayman , Sharma Jaspreet , Rohmer Christina , Uyanga Nomin , Bukkapatnam Sanjana , Van Court Benjamin , Raben David , Young Christian , Heasley Lynn , Karam Sana D.
Published: July 2018

Eph proteins have emerged as critical drivers affecting tumor growth and progression in human malignancies. Our The Cancer Genome Atlas (TCGA) data analysis showed that EphB3, a receptor tyrosine kinase, is frequently coamplified with PIK3CA in head and neck squamous cell carcinoma (HNSCC). We therefore hypothesized that EphB3 amplification plays a protumorigenic role in HNSCC and that EphB3 and PIK3CA are cooperating oncogenes that contribute toward its pathogenesis. This hypothesis was not experimentally supported, because EphB3 knockdown failed to alter HNSCC tumor cell growth in vitro or in vivo with an orthotopic model. However, responsiveness of EphB3 knockdown tumors to the PI3K inhibitor, BKM120, was significantly decreased in terms of both tumor growth delay and survival. This is correlated with an increase in prosurvival proteins, S6 and BcL-XL, in the EphB3 shRNA tumors treated with BKM120 compared with controls. We further observed that EphB3 knockdown resulted in increased migration in vitro and increased EMT gene signature in vivo. To explain these results, we examined EphB3 phosphorylation levels in HNSCC at baseline. Although total EphB3 levels were high, we found low phospho-EphB3 levels in HNSCCs. Forced EphB3 phosphorylation with an ephrin-B2–Fc fusion protein resulted in decreased HNSCC migration and cell growth, and enhanced response to BKM120 in vitro. These data collectively indicate that progression of HNSCC selects for low/inhibited EphB3 activity to enhance their survival and migratory abilities and decrease response to PI3K signaling. Therefore, strategies focused on activating EphB3 might be helpful to inhibit tumor growth and enhance sensitivity to PI3K inhibitors in HNSCC. Mol Cancer Ther; 17(9); 2049–59. ©2018 AACR.
Current Guidelines for Obesity Prevention in Childhood and Adolescence
Authors: Weihrauch-Blüher Susann , Kromeyer-Hauschild Kartin , Graf Christine , Widhalm Kurt , Korsten-Reck Ulrike , Jödicke Birgit , Markert Jana , Müller Manfred James , Moss Anja , Wabitsch Martin , Wiegand Susanna
Published: July 2018

Objective: Current guidelines for prevention of obesity in childhood and adolescence are discussed. Methods: A literature search was performed in Medline via PubMed, and appropriate studies were analyzed. Results: Programs to prevent childhood obesity have so far remained mainly school-based and effects have been limited. Analyses by age group show that prevention programs have the best results in younger children (<12 years). Evidence-based recommendations for pre-school- and early school-aged children indicate the need for interventions that address parents and teachers alike. During adolescence, school-based interventions proved most effective when adolescents were addressed directly. To date, obesity prevention programs have mainly focused on behavior-oriented prevention. Recommendations for community- or environment-based prevention have been suggested by the German Alliance of Noncommunicable Diseases and include a minimum of 1 h of physical activity at school, promotion of healthy food choices by taxing unhealthy foods, mandatory standards for meals at kindergartens and schools as well as a ban on unhealthy food advertisement aimed at children. Conclusion: Behavior-oriented prevention programs showed only limited long-term effects. Certain groups at risk for the development of obesity are not reached effectively by current programs. Although universally valid conclusions cannot be drawn given the heterogeneity of available studies, clearly combining behavior-based programs with community-based prevention to counteract an ‘obesogenic environment' is crucial for sustainable success of future obesity prevention programs.
Topical Eugenol Successfully Treats Experimental Candida albicans-Induced Keratitis
Authors: Hassan Heba A. , Geniady Mahmoud M. , Abdelwahab Sayed F. , Abd-Elghany Manal I. , Sarhan Hatem A. , Abdelghany Ahmed A. , Kamel Mohamed S. , Rodriguez Alejandra E. , Alio Jorge L.
Published: July 2018

Purpose: To determine the efficacy of eugenol for the treatment of Candida keratitis in an experimental model. Methods: The in vitro antifungal activity of eugenol and fluconazole was tested against C. albicans strains via the microbroth dilution method. An experimental model of Candida albicans keratitis was used. Rabbits were classified into those that received no treatment (control; group 1) and those that started eugenol treatment immediately (group 2) or after 4 days (group 3) of keratitis induction (n = 12–16 rabbits/group). The 2 treatment groups were assigned to 50 μL of 4 mg/mL eugenol drops hourly for 15 days, while the control group received saline. Corneal penetration of eugenol was measured using HPLC, and corneal toxicity was evaluated clinically and histopathologically. Results: The in vitro minimum inhibitory concentrations of eugenol and fluconazole against C. albicans were 2 and > 0.4 mg/mL, respectively. A 4-mg/mL preparation of eugenol in propylene glycol was the maximum nontoxic dose on rabbit corneas as suggested by clinical and histopathologic findings. At least 75% of all eugenol-treated eyes recovered from keratitis, with improvement in the remaining 25% of the eyes compared to controls. Conclusions: Eugenol can act as a natural, safe, and effective treatment for fungal keratitis, regardless of whether treatment is started immediately or after 4 days of keratitis induction.
Is Human Sebum the Source of Skin Follicular Ultraviolet-Induced Red Fluorescence? A Cellular to Histological Study
Authors: Xu De-Tian , Yan Jian-Na , Liu Wei , Hou Xiao-Xiao , Zheng Yan , Jiang Wei-Wei , Ju Qiang , Zouboulis Christos C. , Wang Xiu-Li
Published: July 2018

Background: The ultraviolet-induced red fluorescence (UVRF) from human skin follicles was suggested to be a result of Propionibacterium acnes and was used for the monitoring of acne. More recent studies suggested that the UVRF may be more related to sebum rather than to microorganisms. Objective: To clarify whether human sebum or follicular microorganisms are the source of UVRF. Methods: We examined the fluorescence of human-derived SZ95 sebocytes, human sebaceous glands, sebum extracted from the sebaceous glands, and bacteria isolated from human hair follicles under ultraviolet light. Results: SZ95 sebocytes, human sebaceous glands, and sebum do not emit UVRF. Two types of UVRF peaking at about 635 nm and at about 620 nm were detected in P. acnes and Staphylococcus epidermidis, respectively. This is the first report that S. epidermidis emits UVRF when it is anaerobically cultured and then exposed to air. Conclusion: Human follicular UVRF is emitted by resident bacteria, not by sebum. Therefore, UVRF may be used to monitor certain species of skin microorganisms.
Specifications of Computerized Provider Order Entry and Clinical Decision Support Systems for Cancer Patients Undergoing Chemotherapy: A Systematic Review
Authors: Rahimi Rezvan , Kazemi Alireza , Moghaddasi Hamid , Arjmandi Rafsanjani Khadijeh , Bahoush Gholamreza
Published: July 2018

Objective: Chemotherapy medication errors are catastrophic. The prescription phase in the chemotherapy process plays a key role in the creation of medication errors, and therefore the use of computerized physician order entry (CPOE) and clinical decision support system (CDS) systems is recommended to reduce chemotherapy medication errors. The purpose of this study was to carry out a systematic review on the specifications of the CPOE and CDS systems for chemotherapy prescription. Materials and Methods: A systematic review on articles published in English up to September 22, 2017, using the 3 databases PubMed, Embase, and Medline was conducted. Those articles that focused on the specifications of CPOE and CDSS in chemotherapy prescription were included in this review. Findings: Of the 2,471 articles identified, 58 articles met the inclusion criteria and were included in this study. Specifications related to chemotherapy CPOE systems were categorized into the following 6 groups: automation and facilitation of the chemotherapy prescription phase, hospital workflow support, documentation and reporting, drug safety, information security, and system communications. The specifications of chemotherapy CDSS were also divided into 4 categories: embedding chemotherapy protocols, automated dose calculations and adjustment, providing alerts/reminders at the time of prescribing, and guiding or asking the user to complete the important prescription parameters. In 12 articles, the chemotherapy prescription CDSS were designed and evaluated independently of the CPOE; 45 articles provided prescription chemotherapy CDSS as part of the CPOE system, and in 1 article CPOE was introduced with no CDSS. Conclusion: In complicated settings such as chemotherapy, simplification of the processes is more imperative. The use of chemotherapy CPOE, which includes specifications for helping the medical staff with their workload, encourages the professionals to use such systems and increases the likelihood for success of these systems.