bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of manuka honey on 5-fluorouracil chemosensitivity in colonspheres enriched with cancer stem (-like) cells
Authors: Cianciosi Danila , Regolo Lucia , Battino Maurizio , Giampieri Francesca
Published: September 2021

Manuka honey (MH) has an in vitro chemosensitizing effect against 5-Fluorouracil (5-Fu) in colonspheres enriched with cancer stem (-like) cells (CSCs-like); these interesting findings, although preliminary, suggest a potential synergistic action of MH with 5-Fu, which could be useful in deepening the studies on natural compounds to be used in association with conventional medical therapies.
Towards reliable in vitro models – New bi-compartmental platforms for replicating tissue barriers
Authors: Coppadoro Lorenzo P. , Pederzani Elia , Foglieni Chiara , Lombardi Maria , Fiore Gianfranco B. , Soncini Monica
Published: September 2021

In vitro cell cultures represent a widespread methodology for pathophysiology studies with a high benefit/cost ratio. We have developed and are validating TToP, a novel, versatile, and scalable culture system allowing compartmentalized cell/tissue cultures with the aim of mimicking the human pathophysiological microenvironment of tissue barriers.
An in vitro enterocyte cell-like model to study Group B streptococci internalization
Authors: De Gaetano Giuseppe Valerio , Lentini Germana , Galbo Roberta , Coppolino Francesco , Famà Agata , Teti Giuseppe
Published: September 2021

We used a 2D in vitro growth model of Caco2 cells to study invasion of Streptococcus agalactiae. This model can be useful for evaluating the virulence of the most relevant enteric pathogens.
Promising 3D in vitro models for studying tumour heterogeneity and testing novel therapeutic approaches in pancreatic cancer
Authors: Forciniti Stefania , Cavo Marta , Dando Ilaria , Dalla Pozza Elisa , Palmieri Marta , Del Mercato Loretta L.
Published: September 2021

In this study we produced 3D organotypic cultures and spheroids to mimic the complex microenvironment of pancreatic cancer and to test alternative therapeutic strategies.
Light-based 3D printing of functional polydimethylsiloxane- based microfluidic chips
Authors: Gonzalez Gustavo A. , Chiappone Annalisa , Dietliker Kurt , Fabrizio Pirri C. , Roppolo Ignazio
Published: September 2021

The objective of the work is to fabricate and functionalize 3D printed PDMS-based microfluidic chips through digital light processing DLP-3D printing.
Human tissue culture of osteochondral defect: An advanced in vitro model
Authors: Maglio Melania , Tschon Matilde , Pagani Stefania , Vivarelli Leonardo , Govoni Marco , Rocchi Martina , Mazzotta Alessandro , Martini Lucia , Dallari Dante , Fini Milena
Published: September 2021

The optimization of advanced in vitro models is essential for the development of alternative methods. The in vitro study of osteochondral regeneration still has numerous limitations and wide scope for exploration.
Innovative 3D proteome-wide scale identification of ALKBH5 target for MV1035 small molecule able to reduce migration and invasiveness in U87 glioblastoma cell lines by SPILLO-PBSS
Authors: Malacrida Alessio , Rivara Mirko , Mariem Omar Ben , Di Domizio Alessandro , Cislaghi Giacomo , Miloso Mariarosaria , Zuliani Valentina , Nicolini Gabriella
Published: September 2021

The innovative in silico technologies developed at SPILLOproject,1 e.g., the SPILLO potential binding sites searcher (SPILLO-PBSS) software,2,3 allow to identify targets and off-targets of any small molecule on a multiple-organism proteomewide scale, and to perform an accurate multilevel cross-organism transferability analysis (MCOTA) aimed at rationalising animal testing. SPILLO-PBSS has been successfully used in several research projects, such as a study in which a compound (MV1035) was found to reduce migration and invasiveness in U87 glioblastoma (GBM) cell lines: the human structural proteome was analyzed and the RNA demethylase ALKBH5 has been identified as a target responsible for the observed effects (target experimentally validated). Another top-ranked target identified by SPILLO-PBSS, the DNA repair protein AlkB homolog 2 (ALKBH2), abundantly expressed in GBM cell lines, resulted particularly interesting for its pivotal role in the onset of resistance to Temozolomide (TMZ), the standard firstline treatment for GBM.2
Toward scalable in vitro models: a novel experimental and computational pipeline for the identification of cellular metabolic parameters
Authors: Mancini Piera , Botte Ermes , Magliaro Chiara , Ahluwalia Arti
Published: September 2021

Oxygen utilization by cells has a crucial role in the design of advanced in vitro models. The aim of this study is to develop an experimental and computational pipeline for identifying oxygen metabolism parameters. We applied the approach to HepG2 cell monolayer cultures, demonstrating that such parameters depend on cell density.
V-ATPase as a mediator of treatment resistance in Glioblastoma Multiforme: an in vitro study to investigate new therapeutic strategies
Authors: Messuti Eleonora , Giambra Martina , Redaelli Serena , Di Cristofori Andrea , Giussani Carlo , Bentivegna Angela
Published: September 2021

Recent evidences suggest the involvement of the Vacuolar H+ ATPase (V-ATPase) in the development and/or progression of Glioblastoma Multiforme (GBM). This proton pump could be a valid therapeutic target but more in-depth studies are necessary. The aim of this study is to better define the in vitro effects on Glioma Stem Cell (GSC) primary cultures viability of single and combined treatment with Bafilomycin-A1 (Baf-A1), a V-ATPase inhibitor, and Temozolomide (TMZ), the chemotherapeutic agent currently used to treat GBM patients. We found out that GSC were resistant to TMZ and more sensitive to treatments with Baf-A1 and that the two drugs exerted a synergistic effect when administered together.
Light processable starch hydrogels
Authors: Noè Camilla , Tonda-Turo Chiara , Chiappone Annalisa , Sangermano Marco , Hakkarainen Minna
Published: September 2021

Light processable hydrogels were successfully fabricated by utilizing maize starch as raw material. Increasing the starch content from 10 to 15 wt% increased the compressive stiffness from 13 to 20 kPa, which covers the stiffness of different body tissues.
Cadmium elicits alterations in mitochondrial morphology and functionality in C3H10T1/2Cl8 mouse fibroblast cell
Authors: Oldani Monica , Milani Chiara , Gaboardi Federico , Manzoni Marta , Villa Anna M. , Stefanini Federico M. , Melchioretto Pasquale , Monti Eugenio , Forcella Matilde , Urani Chiara , Fusi Paola
Published: September 2021

In this work we exploit the widely used, well known CTA, which allows following healthy cells transformation into a malignant phenotype, to understand the early metabolic deregulation and events in cadmium-induced carcinogenesis.
Chilled storage of zebrafish embryos: Development of an efficient protocol
Authors: Sampieri Maria , Molica Colella Erika , Dotti Silvia
Published: September 2021

Making the zebrafish embryo available to be able to share it between different laboratories groups could pave the way for this animal model with the aim of disseminating and promoting the use and application of zebrafish in scientific research.
In silico experiments as a tool to reduce preclinical tests of magnetic hyperthermia
Authors: Vicentini Marta , Vassallo Marta , Ferrero Riccardo , Manzin Alessandra
Published: September 2021

In silico models can be useful tools to guide preclinical tests of magnetic hyperthermia, which employs Magnetic Nanoparticles (MNPs) excited by AC magnetic fields, as heat mediators for cancer cure. We virtually reproduce the heating process induced by magnetic hyperthermia in murine models, as a function of field applicator features, properties and size of target tissue, MNP dose and animal size.
Polyvinyl alcohol/cellulose hydrogel as possible corneal stroma substitute in drug permeation tests
Authors: Zucchetti Erica , Ling Leonardo , Monti Daniela , Chetoni Patrizia , Tampucci Silvia , Burgalassi Susi
Published: September 2021

The permeation studies of active compounds and formulations are necessary to verify the capability of molecules to pass through the physiological barrier of our body. In order to develop a 3D model of the cornea, preparation and characterization of different hydrogels as corneal stroma substitutes were tested.
Review of African swine fever outbreaks history in South Africa: From 1926 to 2018
Authors: Mushagalusa Ciza A. , Etter Eric , Penrith Mary-Louise
Published: September 2021

The article reviews the outbreaks and distribution of African swine fever (ASF) in South Africa since the first probable outbreak that occurred in the Koedoesrand Ward in 1926. Retrospective data on the ASF outbreaks in South Africa were obtained from the World Organisation for Animal Health (OIE) disease database and the South African veterinary services annual reports in addition to published articles and online sources. South Africa has experienced many outbreaks that can be divided into 2 time periods: the period before the development of the OIE diseases database (1993) and the period after. More than 141 outbreaks of ASF were reported during the first period. Since the development of OIE disease database, 72 outbreaks directly involving 2968 cases, 2187 dead and 2358 killed pigs mainly in smallholder pig farms were reported. The median number of cases for a given ASF outbreak is 17, but in 50% of outbreaks no pigs were killed for prevention. The most important ASF outbreak was reported in April 2014 in the Greater Zeerust district (North West province) involving 326 cases and 1462 killed pigs. However, the outbreak with highest mortality involving 250 pigs was reported in 2016 (Free State province). According to phylogenetic analysis, nine p72 genotypes (I, III, IV, VII, VIII, XIX, XX, XXI and XXII) have been identified in South Africa. Season-wise, more outbreaks were recorded during summer. It was also observed that the OIE disease database could contain errors that would have been introduced through compiled forms at country level. Spatiotemporal studies on ASF outbreaks in South Africa are therefore required in order to assess statistically and quantitatively the clustering of outbreaks over space and time.
Evaluation of root canal morphology of mandibular premolars using cone-beam computed tomography in Rajasthan subpopulation - A retrospective study
Authors: Mittal Neha , Mital Prachi , Prasad Ashwini B. , Raisingani Deepak , Poonia Lalita , Udawat Garima
Published: September 2021

Aim:

The purpose of this study was to evaluate root canal morphology in mandibular first and second premolars among Rajasthan subpopulation in Jaipur city using cone-beam computed tomography (CBCT).

Materials and Methods: CBCT scans of 740 fully erupted mandibular first and second premolars were taken and assessed by two endodontists. Inter-examiner reliability was determined.

Results:

The root canal morphology of 380 permanent mandibular first premolars was assessed which showed canal configurations of Type I in 73.5%, Type V in 11.57%, Type IV in 8.42%, Type III in 2.63%, Type II in 2.1%, Type VII in 2.1% and Type VI and VIII 0% of the scans. The root canal morphology of 360 permanent mandibular second premolars was assessed which showed canal configurations of Type I in 88.88%, Type II in 7.77%, Type IV in 2.2%, Type III and Type V in 0.55%, Type VI, VII, VIII in 0% respectively of the scans.

Conclusion:

The majority of the population in Rajasthan have Vertucci's Type I configuration in both mandibular first and second premolars. However, other canal configurations can also be found in these teeth.
Microbiologic evaluation of cotton, polytetrafluoroethylene tape, and foam as an endodontic spacer material in permanent premolars and molars
Authors: Pisal Nidhi Surendra , Shah Nimisha Chinmay , Dedania Meetkumar S , Bajpai Namrata Anil , Gandhi Namita
Published: September 2021

Aims:

Microbiologic evaluation of cotton, polytetrafluoroethylene (PTFE) tape, and foam as an endodontic spacer material in permanent premolars and molars – an ex vivo study.

Subjects and Methods:

After ethical approval, 72 patients who signed the informed consent form participated in the study. Following access opening and biomechanical preparation, the teeth were randomly assigned to cotton (n = 24), PTFE tape (n = 24), and foam group (n = 24). Samples obtained from the access cavity at baseline (sample one – S1) and after seven days (sample two – S2) were checked for an increase in the microbial load. They were placed on brain-heart infusion agar plates, incubated aerobically for 48 h, and the colony-forming units were calculated.

Statistical Analysis:

One-way analysis of variance and Tukey's post hoc test were used and a P < 0.05 was considered statistically significant.

Results:

There was a statistically significant difference in all the three groups concerning baseline and after Seven-day mean values. An intergroup comparison revealed a statistically significant difference in the mean difference values between the cotton and PTFE tape group. Whereas, there was no statistically significant difference in the mean difference values between the cotton and foam and PTFE tape and foam group.

Conclusions:

Within the limitations of the study, it can be stated that the PTFE tape and foam group performed better than cotton.
Spectrophotometric analysis of crown discoloration induced by various intracanal medicaments
Authors: Parikh Amishi Amit , Nirupama D N , Naveen D N , Sindhu J , Nainan Mohan Thomas
Published: September 2021

Aim:

The aim of the study was to assess discoloration potential of four different intracanal medicaments.

Materials and Methods:

Thirty single-rooted extracted bovine incisors were sectioned to a standardized root length, accessed, instrumented, and placed with temporary filling and cotton pellet. The specimens were divided into five groups (n = 6): Control group with no intracanal medicament and four groups with the following medicaments, i.e., calcium hydroxide (Ca(OH)2), calcium hydroxide with chlorhexidine (Ca(OH)2 with CHX), triple antibiotic paste (TAP), and silver diamine fluoride (SDF), respectively. Calorimetric evaluation, according to CIE L*a*b* system, was performed at pretreatment, on day 1, 7, 14, and 21. Data were analyzed using One-Way ANOVA and Repeated ANOVA. P <0.05 was considered statistically significant.

Results:

The greatest color change (ΔE*) was observed at day 21 in TAP group (P < 0.001) followed by SDF group (P < 0.013), whereas Ca(OH)2 and Ca(OH)2 with CHX groups did not induce visible coronal discoloration.

Conclusions:

Ca(OH)2 and Ca(OH)2 with the CHX groups showed the smallest coronal discoloration during the experimental time, whereas TAP and SDF groups showed the highest coronal discoloration.
Analysis of root and canal morphologies of maxillary second molars in a South Indian population using cone-beam computed tomography
Authors: Rosaline Hannah , Kanagasabai Arivunithi , Shaji Athira , Bose Shreya , Saeralaathan Sindhu , Ganesh Arathi
Published: September 2021

Aim:

The aim of the present study was to analyze the morphological variations and complexities in root canal systems of the maxillary second molars in South Indian population, using cone beam computed tomography (CBCT).

Subjects and Methods:

The CBCT images of 500 participants were examined at different slice thickness of 125 micrometers. Root canal systems of the 500 maxillary second molars were analyzed according to Vertucci, HMA Ahmed et al., and Kim et al. classifications in the Indian subpopulation.

Statistical Analysis Used:

Interobserver variability was analyzed using Kappa statistics.

Results:

Among the tooth analyzed, 63% had three roots, with a single canal in each root in 65.2% tooth. About 41.8% of the tooth had fusion of mesiobuccal and distobuccal roots. The most commonly found root canal configuration was Type I (65%) according to Vertucci's classification. According to HMA Ahmed et al. classification, 67.9% tooth had the configuration of 327 MB1 DB1 P1/317 MB1 DB1 P1. Nearly 20.8% tooth had MB2 canal. The most common isthmus type was Type II (55.2%) according to Kim's classification, and the prevalence of isthmus was 2–5 mm from the root apex.

Conclusions:

The number of roots, canals, their configuration, and occurrence of isthmus has not been reported in the South Indian population. The importance of understanding the knowledge of the anatomy of teeth during the treatment of maxillary second molars would contribute to successful endodontic outcomes.
Predictive role of neutropenia under crizotinib treatment in ALK-rearranged nonsmall cell lung cancer patients
Authors: Gürsoy Pınar , Çakar Burcu , Nart Deniz , Göker Erdem
Published: September 2021

Background:

Anaplastic lymphoma kinase (ALK)-rearranged nonsmall cell lung cancer (NSCLC) represents a molecular subgroup with high sensitivity to ALK inhibitors. Tyrosine kinase inhibitor crizotinib, an anticancer drug acting as an ALK inhibitor, has shown remarkable response in ALK-positive NSCLC. The aim of our study is to explore the adverse events (AEs) of patients on crizotinib therapy and analyze the predictability of AEs for better survival or response on NSCLC patients.

Methods:

The medical records of our ALK-positive metastatic NSCLC patients who applied between years 2013 and 2018 had been reviewed retrospectively. ALK positivity of all patients had been detected by fluorescence in situ hybridization and no other driver mutations were present. Patient demographics, performance status, smoking history, previous treatments, metastatic sites, and AEs were recorded for further analyses.

Results:

Thirty-six ALK-positive metastatic NSCLC patients were included in the study. Median follow-up was 30.1 months. Median progression-free survival (PFS) for patients who developed hepatic, cardiac, or endocrine toxicities was similar when compared to patients who did not develop. Although there was a numeric median PFS difference between patients who did develop visual disorders (18.4 months) and did not develop visual disorders (15.5 month), this was not regarded as statistically significant. However, median PFS of the patients who developed neutropenia upon crizotinib treatment (31.9 months) was found to be more favorable than the patients with normal neutrophil counts (12.8 months) (P = 0.026).

Conclusion:

Neutropenia under crizotinib treatment was found to be associated with improved PFS suggesting that neutropenia might be an important determinant in treatment and survival strategies.
Posttransplant Renal Allograft Dysfunction – A Retrospective Observational Study
Authors: Kunthara Mathew George , Sahay Manisha , Hussain Hakim Imran , Ismal Kiranmai , Vali P Sharmas , Kavadi Anuradha , Kumar B. Vikram
Published: September 2021

Background:

In India, a large number of end-stage renal disease patients are undergoing renal replacement therapy. A successful renal transplantation relives the burden of dialysis with improved quality of life and a productive life thereafter. This also reduces the cost of health care to the government and the society. Graft dysfunction is an important cause of graft loss. The objective of this retrospective study is to evaluate the graft dysfunction and its impact on patient and graft survival.

Methods:

We did a retrospective record-based analysis of 83 cases (including both deceased and live-related renal transplants) from 2014 to 2019 who were on triple immunosuppression (tacrolimus, mycophenolate mofetil, and steroids) as maintenance therapy. Patients who had graft dysfunction, underwent graft biopsy and were analyzed subsequently.

Results:

The most common causes for graft dysfunction on biopsy were acute rejection, acute tubular injury, and calcineurin inhibitor toxicity. About 39% of the patients had infections, predominantly bacterial and viral infections. The rejections were associated with poor patient survival (statistically significant). The overall patient survival at our center after 1 year and 3 years was 88% and 84%, respectively, while the death-censored graft survival was 86% and 81%, respectively.

Conclusion:

In our center, following renal transplantation, patients had a fairly successful outcome. However, early detection and prompt management of the graft dysfunction can improve the graft and the patient survival.
Clinical Characteristics and Outcome of Novel Coronavirus Disease in Kidney Transplant Recipients
Authors: Arumugam Venkatesh , Sastry Bhagavatula V. R. H. , Solomon Dolphin J. , Gopalakrishnan Natarajan , Kannan Badri Srinivasan , Shankar P. , Alavudeen N. Sheik , Dakshinamoorthy Shivakumar , Natarajan Malathy
Published: September 2021

Background:

Organ transplant recipients are at increased risk of infections which may result in acute graft dysfunction and death. Coronavirus disease (COVID-19) is the ongoing global infectious challenge and little is known about the impact of this novel virus in kidney transplant recipients. We here describe the clinical presentations, laboratory profile, and outcomes of 42 such patients, from a large tertiary care center in south India.

Materials and Methods:

This prospective, observational study included all renal transplant recipients with confirmed COVID-19 by reverse transcription polymerase chain reaction from the start of the outbreak till August, 2020. Clinical features at presentation, laboratory and radiological data, and outcomes were analyzed.

Results:

Forty-two patients were included in the analysis. As many as, 86.7% patients of our cohort had symptoms at presentation, with the most common symptoms being fever (52.5%), breathlessness (50%), and cough (40.5%). Significant need for respiratory support was noted in individuals with longer duration posttransplant (P < 0.03). Acute worsening of allograft function was observed in 22 (52.4%) patients. Fourteen (65%) of them had acute on chronic graft dysfunction and acute graft dysfunction was noted in 8 (35%) patients. Six patients (14.5%) died due to the disease and none of the parameters were found to be an independent predictor of mortality in regression analytic models including acute graft dysfunction (P = 0.49) or acute on chronic graft dysfunction (P = 0.07). There was no correlation between disease severity and baseline immunosuppressive agents whether tacrolimus or cyclosporine (P = 0.57) and mycophenolate mofetil or azathioprine (P = 0.91).

Conclusions:

Our largest cohort of patients from India showed higher incidence of acute graft dysfunction and significant mortality in patients with COVID-19.
Pretransplant Compatibility Tests in Kidney Transplants
Authors: Rajvanshi Chhavi , Tiwari Aseem K. , Pabbi Swati , Aggarwal Geet , Saini Abhishek , Chauhan Rajni , Mehra Simmi , Kumari Poonam
Published: September 2021

Pre-transplant compatibility work-up has evolved tremendously from conventional CDC only to CDC with more sensitive FCXM testing with bead-based assays (LMX and SAB). Detection of alloantibodies is one of the major objective in work-up algorithm. Conventionally cell-based tests were used, if both are negative, hospital usually proceeds for transplantation. If one of the screening tests (CDC/FCXM) is or both (CDC and FCXM) screening tests are positive, antibody identification is performed on Luminex platform to determine DSA by virtual cross-match. However, cell-based tests have their limitations. Here, we described a case which could have gone unnoticed if HLA bead-based assay was not there and could have triggered AMR (Antibody mediated rejection) as detected antibody (DRB1*07:01; BCM is 16416) was DSA.
Value of Ambulatory Blood Pressure Monitoring in Potential Renal Donors - A Prospective Observational Study
Authors: Parikh Mital Dipakkumar , Hegde Umapati , Rajapurkar Mohan , Gang Sishir , Konnur Abhijit , Patel Hardik
Published: September 2021

Introduction:

Hypertension is very common among urban as well as rural population. In our donor selection policy, uncontrolled hypertension or target organ damage is a contraindication to kidney donation. Studies of blood pressure (BP) response to unilateral nephrectomy in kidney donors in short- and long-term are needed. We studied BP in kidney donors by ambulatory BP monitoring (ABPM) and the effect of unilateral nephrectomy on BP in them for 1 year following kidney donation.

Methodology:

We enrolled 80 consenting kidney donors aged between 20 and 65 years and followed them serially at pre, 6, and 12 months postkidney donation. Estimated glomerular filtration rate (eGFR) was measured using chronic kidney disease exocrine pancreatic insufficiency (CKD EPI) formula. BP was measured in the clinic (sphygmomanometer) and by ABPM (MeditechABPM05). eGFR, two-dimensional echocardiography, ophthalmological fundus examination, and 24 h proteinuria measurement were done at each visit.

Results:

There was a significant difference in predonation systolic BP by clinic and ABPM (138.07 + 5.5 vs. 117.17 + 10.2; P < 0.001) suggestive of white coat hypertension (WC) effect in 32 donors (40%) which decreased to only 21 (26.25%) donors having WC effect at 12 months of follow-up. There was masked hypertension (MH) in total 3 (3.75%) donors with sustained hypertension (SH) in 10 (12.5%) donors. BP was stable by ABPM till 1-year postdonation. Mean systolic and diastolic BP by ABPM was significantly higher in SH and MH groups as compared to WC and sustained normotensives. Baseline predonation eGFR measured by CKD-EPI was 106.0 + 12.35. eGFR decreased to 80.15 ± 15.76, i.e. 76% of the baseline eGFR. Hypertension by ABPM at baseline was associated with more decrease in the eGFR at 1 year of follow-up particularly in those with age > 50 years and higher body mass index. Proteinuria increased from the baseline value of 82.7 ± 29.94–104.07 ± 49.36 mg/day at 1 year of follow-up.

Conclusions:

ABPM provides a more accurate and reproducible estimate of BP compared to clinic BP to rule out WC hypertension and MH in kidney donors. Unilateral nephrectomy has no significant impact on kidney function or proteinuria over 1 year.
Eosinophilic solid cystic renal cell carcinoma
Authors: Sakhadeo Uma , Yadav Subhash C. , Bakshi Ganesh Kailsas , Prakash Gagan , Katdare Aparna , Menon Santosh , Desai Sangeeta B.
Published: September 2021

Eosinophilic solid cystic renal cell carcinoma (ESC-RCC) is a recently described entity, which demonstrates distinct clinical, pathological and molecular features. We present a series of three cases, the first to be reported from the Indian subcontinent. All three patients were over 50 years of age; and presented with a large kidney mass. One patient had a locally advanced disease while the other two presented with metastases. Microscopic examination revealed a tumor displaying solid-cystic and/or papillary areas composed of clear as well as eosinophilic cells in all three cases. On immunohistochemistry, all the three cases showed a unique CK20+/α-methyl-acyl-CoA-racemase + immunophenotype. Melan-A was focally positive in Case 2. Cytokeratin 7 was focally but strongly positive in Case 3. The two patients with metastatic disease were diagnosed on core biopsies and were advised oral tyrosine kinase inhibitor therapy. The third patient underwent upfront radical nephrectomy. Due to its peculiar morphology and immunoprofile, the diagnosis of ESC-RCC can be confidently made even on a core biopsy. Most cases reported till date had an indolent course. The metastatic presentation in two of our patients emphasizes the need to gather further evidence to ascertain the biological behavior of this emerging entity.
The future of the artificial kidney
Authors: Nagasubramanian Santhosh
Published: September 2021

End-stage renal disease (ESRD) is increasing worldwide. In India, diabetes mellitus and hypertension are the leading causes of chronic kidney disease and ESRD. Hemodialysis is the most prevalent renal replacement therapy (RRT) in India. The ideal RRT must mimic the complex structure of the human kidney while maintaining the patient's quality of life. The quest for finding the ideal RRT, the “artificial kidney”– that can be replicated in the clinical setting and scaled-up across barriers– continues to this date. This review aims to outline the developments, the current status of the artificial kidney and explore its future potential.
Relapsing Polychondritis in a Primiparous Women for Elective Lower Segment Caesarean Section
Authors: Chan Chi Ho , Lu Anqi , Mok May Un Sam
Published: September 2021

Relapsing polychondritis is a rare multisystem autoimmune disorder characterized by recurrent, progressive inflammation and destruction of cartilaginous tissue. Respiratory involvement is the major cause of morbidity and mortality. Airway management during anaesthesia in these patients can be challenging and may result in failed oxygenation and death. Increased physiological demand during pregnancy further complicates anaesthesia planning. Collaborative management under a multidisciplinary team of obstetricians, obstetric anaesthetists, rheumatologist, and pulmonologist is essential. We report a case of a parturient with relapsing polychondritis and severe respiratory involvement for caesarean section under combined epidural-spinal anaesthesia and discuss the anaesthetic management based on current literature.
A Community-based Cross-sectional Study on Self-care and Its Role in the Management of Diabetes Among Patients with Type 2 Diabetes Mellitus
Authors: Vasu Dinesh Peraje , Kulkarni Annarao Gunderao , Gangadhar Namratha Kurunji
Published: September 2021

Introduction:

Type 2 diabetes mellitus is one of the most common non-communicable diseases in India. Self-care practices in diabetes are essential for the control of the disease and improvement of quality of life. The objective of this study was to assess the proportion of study subjects practicing self-care practices and to study their influence on glycemic control.

Materials and Methods:

This is a community-based cross-sectional study on 400 diabetic patients selected by probability proportionate to sample size to assess the self-care practices with the help of a semistructured questionnaire after obtaining institutional ethical clearance.

Results:

Majority of the study population were males (62.75%) and belonged to the age group of 40–60 years (60.25%). About 51% of the respondents were diabetic for less than 6 years; 24% and 16% followed a healthful eating plan and exercised regularly, respectively. About 46% were regular in taking medications and 71% checked their blood sugars regularly. Only 0.5% checked their foot and inside of their foot wear regularly.

Conclusion:

Self-care practices were found to be unsatisfactory in most of the domains that were studied. As these practices are essential for the prevention of complications and to provide a better quality of life, more efforts are needed to educate the people about self-care practices.
Type 2 Diabetes Mellitus in Saudi Arabia
Authors: Tanveer Muhammad Saad , Tanveer Muhammad Hassan , Javed Mamoona
Published: September 2021

Objectives:

To review the effectiveness of current clinical interventions and integrated care program, and the cost-effectiveness of currently available pharmaceutical interventions in the management of Type 2 Diabetes Mellitus in Saudi Arabia.

Data Sources and Methods:

A systematic search of MEDLINE, EMBASE, ScienceDirect, CENTRAL, and Google Scholar was conducted to identify the relevant articles. A detailed inclusion–exclusion criterion was developed and implemented to screen the abstracts and full-texts. We extracted study data from eligible studies into a data extraction form and categorized into various themes to answer our research question.

Study Selection and Themes:

Seventeen studies categorized into three themes were included in this review. The evidence was compiled to report the effectiveness of current clinical interventions, integrated care program, and cost-effectiveness of pharmaceutical interventions.

Conclusions:

There is strong evidence of safety and efficacy of BIAsp 30 in T2DM patients. In addition, BIAsp 30 with or without OADs is more cost-effective compared with other pharmaceutical interventions. The integrated care program is more effective in reducing HbA1c in diabetic patients compared with usual care programs; however, the evidence is small and more studies are required.

Recommendations:

Most of the available studies are small cross-sectional studies. There is a dire need to conduct extensive and high-quality studies, with the sample size representative of Saudi T2DM populations, to generate larger data with high-quality evidence to provide more robust evidence in the future.
KeyFinder: An Efficient Minimal Keys Finding Algorithm For Relational Databases
Authors: Demba Moussa
Published: September 2021

In relational databases, it is essential to know all minimal keys since the concept of database normaliza-tion is based on keys and functional dependencies of a relation schema. Existing algorithms for determining keysor computing the closure of arbitrary sets of attributes are generally time-consuming. In this paper we present aneï¬ƒcient algorithm, called KeyFinder, for solving the key-ï¬nding problem. We also propose a more direct methodfor computing the closure of a set of attributes. KeyFinder is based on a powerful proof procedure for ï¬ndingkeys called tableaux. Experimental results show that KeyFinder outperforms its predecessors in terms of searchspace and execution time.
Effect of soaking conditions on properties of flour from sweet potato slices
Authors: Akinoso Rahman , Odusoga Oluyemi Elizabeth , Alaka Abdulquadri
Published: September 2021

Native flour of sweet potato has limited industrial applications. This study investigated effect of soaking sweet potato root in hot water and citric acid concentrations on flour properties. Sweet potato root was soaked at temperatures of 50oC, 60oC and 70oC for 2 h; citric acid concentrations of 1%, 3% and 5% for 1 h, and combination of best samples in each of the first two modification treatments in 50:50 proportions. Treated root was processed into flour using standard procedures. Proximate, functional and pasting properties of the flours were determined using standard methods. The flour was used as major ingredient in production of baked snacks. Treatments had significant effect on proximate, functional and pasting properties of the flour. Moisture content ranged from 6.5 to 9.7%, protein content 1.6 to 3.0%, ash content 0.1 to 3.15%, fiber content 3.6 to 4.2%, and carbohydrate content 82.1 to 84.7%. Water and oil absorption capacities ranged from 2.2 to 3.0 ml/g and 0.8 g/ml to 1.8 g/ml respectively, swelling power (4.32 to 9.23 g/g) and bulk density (0.77 to 0.95 g/ml). Snack produced with flour gotten from 60oC soaked root for 2 h was best in sensory ratings.
Use of polyethylene terephthalate as an Anti-stripping agent in GLASSPHALT
Authors: Murana Abdulfatai Adinoyi , Ndao Ibrahim , Olowosulu Adekunle Taiwo
Published: September 2021

This study evaluates the use of polyethylene terephthalate (PET) in Glassphalt aimed at increased bonding effect of bitumen, reduced stripping and increased in stability and strength. Control mix were prepared with bitumen content of 5.0, 5.5, 6, 6.5 and 7% to determine the optimum bitumen content (OBC). Optimum glass content (OGC) was obtained from samples prepared with glass content of 5, 10, 15, 20, and 25% replacement by weight of fine aggregates. The OBC and OGC were used prepare samples with PET content of 2, 4, 6, 8, and 10% by weight of the OBC. The optimum PET content had a higher stability value of 5.8kN and higher air void of 3.8% when compared to the control mix. Stripping value tests showed that PET modified glassphalt had 0% stripping after a period of 48 hours. Waste PET of 6.6% in 16% glassphalt is recommended for use as an anti-stripping agent.
Antibiotic Resistance Profiling of Salmonella sp. isolated from African Catfish (Clarias gariepinus)
Authors: Fowoyo P.T.
Published: September 2021

The African catfish (Clarias gariepinus) is an important fresh water fish consumed by a large percentage of the populace globally and it may be contaminated by pathogenic bacteria such as Salmonella sp. In this study, a total of fifteen (15) samples of African catfish were collected from different markets in Lokoja, Nigeria. The Salmonella sp. were isolated from the catfish samples by pre-enrichment in peptone water and subsequent inoculation on selective medium namely brilliant-green agar (BGA), bismuth sulphite agar (BSA) and Salmonella-Shigella agar (SSA). The Salmonella isolates were tested for susceptibility to 10 different commercially available antibiotics using the disc diffusion method. A total of thirty-four Salmonella species was isolated. The percentage occurrence of Salmonella sp. in the catfishes examined was very high (80%). The incidence of Salmonella sp. in the intestine (86.7%) of the catfish was higher than for the gills (66.7%) and the skin (73.3%). Majority of the isolates were resistant to Amoxicillin, Sulfomethoxazole-trimethoprim, Amoxicillin-clavulanic acid and Streptomycin. This study therefore demonstrated the occurrence of Salmonella species in African catfish with some exhibiting antibiotic resistance. Thus, there is a potential risk of transmission of drug resistant Salmonella species to man when contaminated catfish is consumed. The use of antibiotics in fish farming should be regulated so as to decrease antibiotic residues in fish.
O mestre, a obra e o amigo:
Authors: Pereira Guimarães Rocha Everardo
Published: September 2021

Iniciar um texto em homenagem ao professor José Carlos Rodrigues poderia ser a partir de qualquer uma das diversas possibilidades que, em nossos mais de quarenta anos de convivência, ele descortinou para mim, seja como amigo, colega de departamento, professor, coautor ou, principalmente, como importante referência bibliográfica em minha formação. Escolho, portanto, esta última, pois sua obra acadêmica e seu projeto intelectual foram essenciais para meu próprio trabalho, bem como para centenas de outros pesquisadores que puderam conhecer seus inspiradores estudos.
O mestre revelado:
Authors: Goldman Marcio
Published: September 2021

que dizer de alguém que se conhece há quase meio século, que foi o primeiro professor da disciplina que passei a vida praticando, depois meu colega e amigo de décadas? O risco, como sempre — e nele já acabo de cair — é falar mais de si mesmo do que da pessoa que aqui se homenageia. Portanto, é com desculpas e um pouco de vergonha que me limitarei a evocar memórias muito antigas, mas que, é claro, continuam muito atuais.
“Conversar” e “engrossar”:
Authors: Rodrigues José Carlos
Published: September 2021

A relação entre as leis e as práticas sociais no Brasil tem sido objeto frequente de reflexão por parte de cientistas sociais, autoridades públicas e mesmo de pessoas comuns. Alguns analistas apontam para a maleabilidade das regras de acordo com os contextos, enquanto outros argumentam em favor do endurecimento dos códigos. Este artigo pretende apresentar uma contribuição ao debate a partir da relativização cultural dessas posições extremas. A pesquisa vem se estendendo por mais de uma dezena de anos, envolvendo trabalho de campo, com aplicação de questionários, entrevistas informais com perguntas abertas e observação etnográfica direta. A orientação teórica principal consiste em considerar certas situações como “dramas sociais” (GLUCKMAN, 1987; TURNER, 1970, 1974, 1975; DAMATTA, 1979, 1985, 1993) em que se buscam soluções de conflitos. Os dados demonstram que a plasticidade das regras no Brasil é um fenômeno cultural, atuante tanto nas esferas macro quanto nas microscópicas da sociedade. Confirmam também que esta maleabilidade não se limita a abrandar os efeitos das leis, correspondendo com frequência ao endurecimento das mesmas.
Construção Social nas Redes:
Authors: Petrik Manuel Luís
Published: September 2021

O artigo traz resultados parciais de tese de doutorado, que contempla a polêmica em comentários de usuários em perfis de jornais brasileiros no Facebook. São analisadas duas semanas de postagens nas páginas da Folha de São Paulo e de O Estado de São Paulo na referida rede social. A base metodológica adotada é a Teoria Fundamentada (GLASER, STRAUSS, 2006). Foram criadas categorias temáticas para as postagens, além de ter sido formulada uma tipologia de comentários. Como resultados mais salientes aparece o grande interesse dos leitores por temas políticos, sucedido por assuntos morais e comportamentais, ao mesmo tempo que, em termos de manifestação dos usuários das redes, destaca-se o tom refratário dos comentários. A junção dessas temáticas com esse modo de expressão do público compõe a reflexão final do texto.
A hora da estrela e suas adaptações
Authors: Miranda Silva Eduardo
Published: September 2021

Tendo como ponto de partida as reflexões de Rodrigo S.M. no romance A Hora da Estrela, de Clarice Lispector, o artigo se propõe a discutir algumas das opções narrativas em duas adaptações audiovisuais da obra literária. Na primeira obra, o filme homônimo A Hora da Estrela, de 1985, a diretora Suzana Amaral opta por focalizar apenas a história de Macabéa, deixando de fora as elucubrações de um narrador culpado. Em um segundo momento, o programa de televisão Cena Aberta, de 2003, do diretor e roteirista Jorge Furtado, ajusta seu foco justamente na problemática de se falar pelo outro de classe. Nossa hipótese inicial sugere uma dificuldade do cinema de destituir o intelectual tradicional de seu poder de narrar, no caso do filme de Suzana Amaral. Mas também aponta para a perda do outrora poder de choque a qual a metalinguagem se propunha. Essa mesma metalinguagem é o instrumento primeiro de fatura utilizado por Jorge Furtado na adaptação de Lispector para o programa da TV Globo.
Application Design of Web-based Computer Laboratory Inventory and Maintenance System at SMK Bhakti Anindya
Authors: Syafrizal Reza , Sarmuni Sarmuni , Aibar Rizki
Published: September 2021

This paper describes a problem in computer laboratory maintenance and inventory. Inventory of goods in Every company or institution always has a connection with the data management process of goods / equipment owned by the company. In addition to inventory, maintenance is also very necessary in order to keep company assets under control, safe, and always maintained. For this reason, an Inventory Information System is needed as well as a maintenance system in managing the data. The results of this design are focused on managing information about the management of goods and several aspects regarding the maintenance of goods, especially in the computer laboratory of SMK Bhakti Anindya. With the implementation of the maintenance information system and laboratory inventory at Bhakti Anindya Vocational School, data management and equipment maintenance will be better.

Tulisan ini menjelaskan mengenai sebuah permasalahan dalam perawatan dan inventaris laboratorium komputer. Inventarisasi barang dalam Setiap perusahaan atau sebuah lembaga selalu memiliki keterkaitan dengan proses pengelolaan data barang / peralatan yang di miliki oleh perusahaan tersebut. Disamping  inventarisasi, hal perawatan juga sangat diperlukan  demi menjaga agar aset perusahaan terkontrol, aman, dan selalu terawat. Untuk itulah di butuhkan suatu Sistem Informasi Inventaris sekaligus sistem perawatannya dalam mengelola data tersebut. Hasil dari rancang bangun ini dititik beratkan pada pengelolaan informasi tentang pengelolaan barang dan beberapa aspek mengenai perawatan barang terutama pada bagian laboratorium komputer SMK Bhakti Anindya. Dengan diimplementasikannya sistem informasi Perawatan dan inventaris laboratorium di SMK Bhakti Anindya, pengelolaan data barang dan perawatan alat akan lebih baik.
Mongolia stage decoration in the socialist period
Authors: Ли Ч.
Published: September 2021

До появления профессиональных театров в Монголии театрализованные представления, мистерии и гимны, танец Цам, церемонии в честь Майтрейи, имеющие религиозное назначение, исполнялись в монастырях Их Хурээ, Эрдэнэ Зуу и некоторых крупных монгольских храмах. Результаты данного исследования показывают, что оформление религиозных и государственных театрализованных церемоний было разработано художниками и мастерами декоративно-прикладного искусства и предназначено для демонстрации на подмостках и на площади. Все типы мистерии были основой для развития декоративно-прикладного искусства, а именно для создания одежды и аксессуаров с активным использованием дерева, кости, камня, металла, кожи, бумаги и войлока. Таким образом, традиционные народные промыслы, такие как шитье, вышивание, ткачество, аппликации, резьба, тиснение и роспись, использовались для изготовления костюмов для Цама, масок и реквизита. Этот факт доказывает, что все виды декоративно-прикладного искусства, связанные с театрализованными церемониями, являются традиционным художественным творчеством. После Народной революции 1921 года в Монголии начало развиваться современное театральное искусство, которое создало условия для развития различных творческих профессий, связанных со сценой.

Prior to the establishment of professional theaters in Mongolia, operas and hymns were performed at the Ikh Khuree, and Tsam dances and Maitreya ceremonies were held at the Ikh Khuree, Erdene Zuu, and some major Mongolian temples. The results of the research show that these works of art, religion, and state ceremonies were designed by artists and craftsmen who were trained in arts and crafts, and were designed to be placed on stage and in the square, and to create clothes and accessories. For example, using wood, bone, stone, metal, leather, paper, and felt, traditional folk crafts such as sewing, stitching, weaving, gluing, carving, embossing, weaving, and painting are used to make Tsam costumes, masks, and stage set. The fact that the Mongolian stage design is a traditional art activity proves that it was a traditional art activity. After the People's Revolution of 1921, Mongolians established modern theatrical art, which created conditions for the development of various professions created on stage. An integral part of the development of the theater is the stage design and decoration. Stage decoration can be considered as a collective work of playwrights, directors, actors, composers and stage artists.
The revival of felt art in Mongolia in the case of the artist Munkhsoyol Batsukh
Authors: Онуфриенко Д.Е.
Published: September 2021

Объектом исследования является творчество одного из мастеров декоративно-прикладного искусства Мунхсоёла Батсуха. В статье рассмотрены вопросы истории развития войлочного искусства Монголии на протяжении веков. Показано, что традиции народных мастеров не утрачены, а активно применяются современными мастерами. В частности, возрождаются приемы, сложившиеся еще в гуннское время. В орнаментации распространены монгольские растительные и геометрические узоры, в сюжетных композициях используются образы народной мифопоэтики. В статье дан анализ трех серий работ художника — крупных ковровых изделий для торжественных правительственных церемоний, «Связь прошлого и настоящего», «Сцены охоты».

The object of the study is the work of Munkhsoyol Batsukh, one of the masters of decorative and applied art. The article considers the issues of the history of the development of the felt art of Mongolia, its development over the centuries. The author showed that the traditions of folk masters are not lost, but are actively used by modern masters. In particular, the techniques that have developed in the Hunnic times are being revived. Mongolian floral and geometric patterns are widespread in ornamentation, images of folk mythic poetics are used in plot compositions. The article analyzes three series of works by the artist — large carpets for solemn government ceremonies, “The connection between the past and the present”, “Hunting scenes”.