bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Gas Pressure during Electron Beam Energy Deposition in the EBM Additive Manufacturing Process
Authors: Damri Elroei , Tiferet Eitan , Braun Dor , Ganor Yaron Itay , Chonin Michael , Orion Itzhak
Published: April 2021

Electron beam melting (EBM) is a metal powder bed fusion additive manufacturing (AM) technology that facilitates the production of metal parts by selectively melting areas in layers of metal powder. The electron beam melting process is conducted in a vacuum chamber environment regulated with helium (He) at a pressure on the scale of 10−3 mbar. One of the disadvantages of vacuum environments is the effect of vapor pressure on volatile materials: indeed, elements in the pre-alloyed powder with high vapor pressure are at risk of evaporation. Increasing the He pressure in the process can improve the thermodynamic stability of the pre-alloyed components and decrease the composition volatility of the solid. However, increasing the pressure can also attenuate the electrons and consequently reduce the energy deposition efficiency. While it is generally assumed that the efficiency of the process is 90%, to date no studies have verified this. In this study, Monte Carlo simulations and detailed thermal experiments were conducted utilizing EGS5 and an Arcam Q20+ machine. The results reveal that increasing the gas pressure in the vacuum chamber by one order of magnitude (from 10−3 mbar to 10−2 mbar) did not significantly reduce the energy deposition efficiency (less than 1.5%). The increase in gas pressure will enable the melting of alloys with high vapor pressure elements in the future.
Ocean Acidification Induces Changes in Virus–Host Relationships in Mediterranean Benthic Ecosystems
Authors: Tangherlini Michael , Corinaldesi Cinzia , Ape Francesca , Greco Silvestro , Romeo Teresa , Andaloro Franco , Danovaro Roberto
Published: April 2021

Acidified marine systems represent “natural laboratories”, which provide opportunities to investigate the impacts of ocean acidification on different living components, including microbes. Here, we compared the benthic microbial response in four naturally acidified sites within the Southern Tyrrhenian Sea characterized by different acidification sources (i.e., CO2 emissions at Ischia, mixed gases at Panarea and Basiluzzo and acidified freshwater from karst rocks at Presidiana) and pH values. We investigated prokaryotic abundance, activity and biodiversity, viral abundance and prokaryotic infections, along with the biochemical composition of the sediment organic matter. We found that, despite differences in local environmental dynamics, viral life strategies change in acidified conditions from mainly lytic to temperate lifestyles (e.g., chronic infection), also resulting in a lowered impact on prokaryotic communities, which shift towards (chemo)autotrophic assemblages, with lower organic matter consumption. Taken together, these results suggest that ocean acidification exerts a deep control on microbial benthic assemblages, with important feedbacks on ecosystem functioning.
Vaccines to Prevent Meningitis: Historical Perspectives and Future Directions
Authors: Alderson Mark R. , Welsch Jo Anne , Regan Katie , Newhouse Lauren , Bhat Niranjan , Marfin Anthony A.
Published: April 2021

Despite advances in the development and introduction of vaccines against the major bacterial causes of meningitis, the disease and its long-term after-effects remain a problem globally. The Global Roadmap to Defeat Meningitis by 2030 aims to accelerate progress through visionary and strategic goals that place a major emphasis on preventing meningitis via vaccination. Global vaccination against Haemophilus influenzae type B (Hib) is the most advanced, such that successful and low-cost combination vaccines incorporating Hib are broadly available. More affordable pneumococcal conjugate vaccines are becoming increasingly available, although countries ineligible for donor support still face access challenges and global serotype coverage is incomplete with existing licensed vaccines. Meningococcal disease control in Africa has progressed with the successful deployment of a low-cost serogroup A conjugate vaccine, but other serogroups still cause outbreaks in regions of the world where broadly protective and affordable vaccines have not been introduced into routine immunization programs. Progress has lagged for prevention of neonatal meningitis and although maternal vaccination against the leading cause, group B streptococcus (GBS), has progressed into clinical trials, no GBS vaccine has thus far reached Phase 3 evaluation. This article examines current and future efforts to control meningitis through vaccination.
Lassa Virus Treatment Options
Authors: Hansen Frederick , Jarvis Michael A. , Feldmann Heinz , Rosenke Kyle
Published: April 2021

Lassa fever causes an approximate 5000 to 10,000 deaths annually in West Africa and cases have been imported into Europe and the Americas, challenging public health. Although Lassa virus was first described over 5 decades ago in 1969, no treatments or vaccines have been approved to treat or prevent infection. In this review, we discuss current therapeutics in the development pipeline for the treatment of Lassa fever, focusing on those that have been evaluated in humans or animal models. Several treatments, including the antiviral favipiravir and a human monoclonal antibody cocktail, have shown efficacy in preclinical rodent and non-human primate animal models and have potential for use in clinical settings. Movement of the promising preclinical treatment options for Lassa fever into clinical trials is critical to continue addressing this neglected tropical disease.
Potentially Bioaccessible Phenolic and Antioxidant Potential of Fresh and Stored Lentil Sprouts—Effect of Lactobacillus plantarum 299v Enrichment
Authors: Gawlik-Dziki Urszula , Baraniak Barbara , Sikora Małgorzata , Jakubczyk Anna , Kapusta Ireneusz , Świeca Michał
Published: April 2021

The phenolic and antioxidant potential of potentially bioaccessible fractions of lentil sprouts was studied. Sprouts were cocultivated with a probiotic to obtain a new functional product and further stored in cool conditions. The fraction obtained after buffer extraction and gastric digestion had higher content of phenolics compared to the control (by 20% and 46%, respectively); however, a 9% decrease was observed in samples obtained after gastrointestinal digestion. After gastrointestinal digestion, the highest content of phenolics (278 µg/g d.w.) was determined in the fresh control sprouts. Compounds neutralizing ABTS and hydroxyl radicals, chelating metal ions, and exhibiting strong reducing power were effectively released after gastrointestinal digestion (e.g., the values of the gastrointestinal digestibility index for chelating power and ability to quench hydroxyl radicals significantly exceeded 1 in all studied samples). It was proved that the enrichment of sprouts with a probiotic and further storage significantly improved the antioxidant potential; compared to the fresh control sprouts, an increase by 45% and 10% was determined after the gastric and gastrointestinal digestion, respectively. Lentil sprouts enriched with L. plantarum 299v may be a new functional product characterized by the high antioxidant capacity of the potentially bioaccessible fraction.
Thermodynamics, Charge Transfer and Practical Considerations of Solid Boosters in Redox Flow Batteries
Authors: Moghaddam Mahdi , Sepp Silver , Wiberg Cedrik , Bertei Antonio , Rucci Alexis , Peljo Pekka
Published: April 2021

Solid boosters are an emerging concept for improving the performance and especially the energy storage density of the redox flow batteries, but thermodynamical and practical considerations of these systems are missing, scarce or scattered in the literature. In this paper we will formulate how these systems work from the point of view of thermodynamics. We describe possible pathways for charge transfer, estimate the overpotentials required for these reactions in realistic conditions, and illustrate the range of energy storage densities achievable considering different redox electrolyte concentrations, solid volume fractions and solid charge storage densities. Approximately 80% of charge storage capacity of the solid can be accessed if redox electrolyte and redox solid have matching redox potentials. 100 times higher active areas are required from the solid boosters in the tank to reach overpotentials of <10 mV.
A New Coumarin-Acridone Compound as a Fluorescence Probe for Fe3+ and Its Application in Living Cells and Zebrafish
Authors: Huang Jiayong , Yan Zhenshuo , Qiu Peiling , Mo Yufeng , Cao Qizhen , Li Qiuhong , Huo Lini , Zhao Lichun
Published: April 2021

A new coumarin-acridone fluorescent probe S was designed and synthesized, and the structure was confirmed with 1H/13C NMR spectrometry, single-crystal X-ray diffraction, and high-resolution mass spectrometry. This probe has high sensitivity and selectivity for Fe3+ over other testing metal ions at 420 or 436 nm in acetonitrile–MOPS (3-Morpholinopropanesulfonic Acid) buffer solution (20.0 μM, pH = 6.9, 8:2 (v/v)). Under physiological conditions, the probe displayed satisfying time stability with a detection limit of 1.77 µM. In addition, probe S was successfully used to detect intracellular iron changes through a fluorescence-off mode, and the imaging results of cells and zebrafish confirmed their low cytotoxicity and satisfactory cell membrane permeability, as well as their potential biological applications.
Pulse Probiotic Superfood as Iron Status Improvement Agent in Active Women—A Review
Authors: Rajagukguk Yolanda Victoria , Arnold Marcellus , Gramza-Michałowska Anna
Published: April 2021

Active women or women of reproductive age (15–49 years old) have a high risk of suffering from anaemia. Anaemia is not solely caused by iron deficiency, however, the approaches to improve iron status in both cases are greatly related. Improving the iron status of active women can be done by dietary intervention with functional food. This review aims to provide insights about the functional food role to increase iron absorption in active women and the potency of pulse probiotic superfood development in dry matrices. Results showed that the beneficial effect of iron status is significantly improved by the synergic work between probiotic and prebiotic. Furthermore, chickpeas and lentils are good sources of prebiotic and the consumption of pulses are related with 21st century people’s intention to eat healthy food. There are wide possibilities to develop functional food products incorporated with probiotics to improve iron status in active woman.
An Exploratory Study on the Impact of Collective Immersion on Learning and Learning Experience
Authors: Du Vignaux Maÿlis Merveilleux , Léger Pierre-Majorique , Charland Patrick , Salame Youness , Durand Emmanuel , Bouillot Nicolas , Pardoen Mylène , Sénécal Sylvain
Published: April 2021

This paper aims to explore the impact of a collective immersion on learners’ engagement and performance. Building on Bandura’s social learning theory and the theory on the sense of presence, we hypothesise that collective immersion has a positive impact on performance as well as cognitive, emotional and behavioural engagement. Ninety-three participants distributed in four conditions took part in the experiment. The four conditions manipulated the collective and individual dimensions of the learning environment as well as the high and low immersion of the learning material. The two conditions that offered a high immersion setting used two types of the novel immersive dome: a large one for collective immersion and a small one for individual use. All participants were presented with the same stimuli, an 8-min-long video of a virtual neighbourhood visit in Paris in the 18th century. The participants’ reactions were measured during and after the task. The learning outcome, as well as the cognitive, emotional and behavioural engagement, were measured. Final results showed that collective immersion learning outcomes are not significantly different, but we find that collective immersion impacts the cognitive, emotional and behavioural engagement of learners.
Integration of an Aerosol-Assisted Deposition Technique for the Deposition of Functional Biomaterials Applied to the Fabrication of Miniaturised Ion Sensors
Authors: Ruiz-Gonzalez Antonio , Choy Kwang-Leong
Published: April 2021

Ion-selective electrodes are at the forefront of research nowadays, with applications in healthcare, agriculture and water quality analysis among others. Despite multiple attempts of miniaturization of these polyvinyl chloride (PVC) gel-based ion sensors, no ion-sensing devices with a thickness below the micrometer range, and operating using open circuit potential, have been developed so far. This work reports the causes of this thickness limitation in potassium-selective sensors. Highly homogeneous ion-sensing films were fabricated by a method based on aerosol assisted chemical vapour deposition, leading to smooth surfaces with 27 ± 11 nm of roughness. Such homogeneity allowed the systematic study of the performance and ionic diffusion properties of the sensing films at sub-micrometer scales. Sensitivities below the Nernst response were found at low thicknesses. The nature of this reduction in sensitivity was studied, and a difference in the superficial and bulk compositions of the films was measured. An optimal configuration was found at 15 µm, with a good selectivity against Na+ (KK+, Na+ = −1.8) a limit of detection in the range of 10−4 M and esponse time below 40 s. The stability of sensors was improved by the deposition of protective layers, which expanded the lifespan of the ion sensors up to 5 weeks while preserving the Nernst sensitivity.
Fabrication of Polymer Composite Fibers Embedding Ultra-Long Micro/Nanowires
Authors: Yang Bo , Pan Dawei , Sun Laixi , Chen Shufan , Wu Weidong , Li Bo
Published: April 2021

Fabrication of polymer composite fibers embedding ultra-long micro/nanowires via an iterative melt co-drawing and bundling technique is reported in this study. The poly(methyl methacrylate) (PMMA) porous array templates were prepared with section-cutting the PMMA/polystyrene (PS) (shell/core) composite fibers and dissolution of inner PS. The results showed that the PS cores or pores in the PMMA matrix are regularly arranged with hexagonal, and their diameter and spacing exhibits a uniform distribution. Especially, the core diameter can be precisely controlled from millimeter-scale to nanometer-scale by multi-step melt co-drawing. Based on the PMMA porous array templates, the Cu nanowires were successfully prepared by electrochemical deposition. Moreover, to fabricate PMMA ultra-long micro/nanowires, the composite fibers with converse shell/core component of PS/PMMA were initially prepared, and then the outer PS was dissolved. The obtained PMMA micro/nanowires were characterized with smooth complete orientation structure. The study provides an experimental basis for fabricating such polymer composite fibers, micro/nano porous array templates, and micro/nanowires with precise and controllable manner to meet the real application requirements.
Social Modeling and Eating Behavior—A Narrative Review
Authors: Suwalska Julia , Bogdański Paweł
Published: April 2021

Social modeling of eating is the adjustment of the amount of food eaten to the intake of the accompanying person. In this paper we provide a narrative review of literature on social modeling of eating with a particular focus on recent studies. Firstly, we describe the structure of a typical modeling experiment. Secondly, we present a variety of research in this field: experiments with various types of confederates, experiments aimed at the evaluation of the influence of gender, partner’s body weight, type of food, hunger, personal characteristics, etc. Thirdly, we present practical implications of this knowledge. The common conclusion is that social modeling of eating occurs in different situations and consumption is adapted to the standards established by the eating partner, but is not their direct reflection. Social influence of eating is not restricted to "artificial" laboratory situations; social modeling and social norms manipulations may be used to change people’s dietary practices, especially in children and young adults. Within the home environment parental modeling has been shown to promote children’s snacking and fruit and vegetable consumption. Social modeling may be used in nutrition interventions aimed at the improvement of children’s diet and in obesity prevention programs.
A Dietary Intervention High in Green Leafy Vegetables Reduces Oxidative DNA Damage in Adults at Increased Risk of Colorectal Cancer: Biological Outcomes of the Randomized Controlled Meat and Three Greens (M3G) Feasibility Trial
Authors: Frugé Andrew D. , Smith Kristen S. , Riviere Aaron J. , Tenpenny-Chigas Rachel , Demark-Wahnefried Wendy , Arthur Anna E. , Murrah William M. , van der Pol William J. , Jasper Shanese L. , Morrow Casey D. , Arnold Robert D. , Braxton-Lloyd Kimberly
Published: April 2021

Green leafy vegetables (GLV) may reduce the risk of red meat (RM)-induced colonic DNA damage and colorectal cancer (CRC). We previously reported the primary outcomes (feasibility) of a 12-week randomized controlled crossover trial in adults with habitual high RM and low GLV intake with body mass index (BMI) > 30 kg/m2 (NCT03582306). Herein, our objective was to report a priori secondary outcomes. Participants were recruited and enrolled in 2018, stratified by gender, and randomized to two arms: immediate intervention group (IG, n = 26) or delayed intervention group (DG, n = 24). During the 4 week intervention period, participants were provided with frozen GLV and counseled to consume 1 cooked cup equivalent daily. Participants consumed their normal diet for the remaining 8 weeks. At each of four study visits, anthropometrics, stool, and blood were taken. Overall, plasma Vitamin K1 (0.50 ± 1.18 ng/mL, p < 0.001) increased, while circulating 8OHdG (−8.52 ± 19.05 ng/mL, p < 0.001), fecal 8OHdG (−6.78 ± 34.86 ng/mL, p < 0.001), and TNFα (−16.95 ± 60.82 pg/mL, p < 0.001) decreased during the GLV intervention compared to control periods. Alpha diversity of fecal microbiota and relative abundance of major taxa did not differ systematically across study periods. Further investigation of the effects of increased GLV intake on CRC risk is warranted.
Rational Vaccine Design in Times of Emerging Diseases: The Critical Choices of Immunological Correlates of Protection, Vaccine Antigen and Immunomodulation
Authors: Schijns Virgil , Majhen Dragomira , van der Ley Peter , Thakur Aneesh , Summerfield Artur , Berisio Rita , Nativi Cristina , Fernández-Tejada Alberto , Alvarez-Dominguez Carmen , Gizurarson Sveinbjörn , Zamyatina Alla , Molinaro Antonio , Rosano Camillo , Jakopin Žiga , Gursel Ihsan , McClean Siobhán
Published: April 2021

Vaccines are the most effective medical intervention due to their continual success in preventing infections and improving mortality worldwide. Early vaccines were developed empirically however, rational design of vaccines can allow us to optimise their efficacy, by tailoring the immune response. Establishing the immune correlates of protection greatly informs the rational design of vaccines. This facilitates the selection of the best vaccine antigens and the most appropriate vaccine adjuvant to generate optimal memory immune T cell and B cell responses. This review outlines the range of vaccine types that are currently authorised and those under development. We outline the optimal immunological correlates of protection that can be targeted. Finally we review approaches to rational antigen selection and rational vaccine adjuvant design. Harnessing current knowledge on protective immune responses in combination with critical vaccine components is imperative to the prevention of future life-threatening diseases.
Assessment and Development of the Antifungal Agent Caspofungin for Aerosolized Pulmonary Delivery
Authors: Yu Iching G. , Ryckman David M.
Published: April 2021

Invasive Pulmonary Aspergillosis (IPA) and Pneumocystis jiroveci Pneumonia (PCP) are serious fungal pulmonary diseases for immunocompromised patients. The brand name drug CANCIDAS® (Caspofungin acetate for injection) is FDA approved to treat IPA, but is only 40% effective. Efficacious drug levels at the lung infection site are not achieved by systemic administration. Increasing the dose leads to toxicity. The objective, here, is to reformulate caspofungin for aerosolization to high drug concentration by lung targeted delivery and avoid systemic distribution. Described in this paper is a new, room temperature-stable formulation that meets these goals. The in vitro antifungal activity, solid state and reconstituted stability, and aerosol properties of the new formulation are presented. In addition, pharmacokinetic parameters and tissue distribution data are determined from nose-only inhalation studies in rats. Plasma and tissue samples were analyzed by High Performance Liquid Chromatography-tandem Mass Spectrometry (HPLC-MS-MS). Inhaled drug concentrations for caspofungin Active Pharmaceutical Ingredient (API), and the new formulation, were compared at the same dose. In the lungs, the parameters Cmax and Area Under Curve (AUC) showed a 70%, and 60%, respective increase in drug deposition for the new formulation without significant systemic distribution. Moreover, the calculated pharmacodynamic indices suggest an improvement in efficacy. These findings warrant further animal toxicology studies and human clinical trials, with inhaled caspofungin, for treating IPA.
An In Vivo Predictive Dissolution Methodology (iPD Methodology) with a BCS Class IIb Drug Can Predict the In Vivo Bioequivalence Results: Etoricoxib Products
Authors: Gonzalez-Alvarez Isabel , Bermejo Marival , Tsume Yasuhiro , Ruiz-Picazo Alejandro , Gonzalez-Alvarez Marta , Hens Bart , Garcia-Arieta Alfredo , Amidon Greg E. , Amidon Gordon L.
Published: April 2021

The purpose of this study was to predict in vivo performance of three oral products of Etoricoxib (Arcoxia® as reference and two generic formulations in development) by conducting in vivo predictive dissolution with GIS (Gastro Intestinal Simulator) and computational analysis. Those predictions were compared with the results from previous bioequivalence (BE) human studies. Product dissolution studies were performed using a computer-controlled multicompartmental dissolution device (GIS) equipped with three dissolution chambers, representing stomach, duodenum, and jejunum, with integrated transit times and secretion rates. The measured dissolved amounts were modelled in each compartment with a set of differential equations representing transit, dissolution, and precipitation processes. The observed drug concentration by in vitro dissolution studies were directly convoluted with permeability and disposition parameters from literature to generate the predicted plasma concentrations. The GIS was able to detect the dissolution differences among reference and generic formulations in the gastric chamber where the drug solubility is high (pH 2) while the USP 2 standard dissolution test at pH 2 did not show any difference. Therefore, the current study confirms the importance of multicompartmental dissolution testing for weak bases as observed for other case examples but also the impact of excipients on duodenal and jejunal in vivo behavior.
Meta-Deflectors Made of Dielectric Nanohole Arrays with Anti-Damage Potential
Authors: Yu Haichao , Tang Feng , Wu Jingjun , Yi Zao , Ye Xin , Wang Yiqun
Published: April 2021

In intense-light systems, the traditional discrete optical components lead to high complexity and high cost. Metasurfaces, which have received increasing attention due to the ability to locally manipulate the amplitude, phase, and polarization of light, are promising for addressing this issue. In the study, a metasurface-based reflective deflector is investigated which is composed of silicon nanohole arrays that confine the strongest electric field in the air zone. Subsequently, the in-air electric field does not interact with the silicon material directly, attenuating the optothermal effect that causes laser damage. The highest reflectance of nanoholes can be above 99% while the strongest electric fields are tuned into the air zone. One presentative deflector is designed based on these nanoholes with in-air-hole field confinement and anti-damage potential. The 1st order of the meta-deflector has the highest reflectance of 55.74%, and the reflectance sum of all the orders of the meta-deflector is 92.38%. The optothermal simulations show that the meta-deflector can theoretically handle a maximum laser density of 0.24 W/µm2. The study provides an approach to improving the anti-damage property of the reflective phase-control metasurfaces for intense-light systems, which can be exploited in many applications, such as laser scalpels, laser cutting devices, etc.
Femtosecond Laser Fabricated Apodized Fiber Bragg Gratings Based on Energy Regulation
Authors: Guo Qi , Zheng Zhongming , Wang Bo , Pan Xuepeng , Liu Shanren , Tian Zhennan , Chen Chao , Yu Yongsen
Published: April 2021

In this paper, an energy regulation method based on the combination of a half-wave plate (HWP) and a polarization beam splitter (PBS) is proposed for the fabrication of apodized fiber gratings, which can effectively improve the side lobe suppression ratio of high-reflectivity fiber Bragg gratings (FBGs) fabricated by femtosecond laser. The apodized FBGs prepared by this method has good repeatability and flexibility. By inputting different types of apodization functions through the program, the rotation speed of the stepping motor can be adjusted synchronously, and then the position of the HWP can be accurately controlled so that the laser energy can be distributed as an apodization function along the axial direction of the fiber. By using the energy apodization method, the gratings with a reflectivity of 75% and a side lobe suppression ratio of 25 and 32 dB are fabricated in the fiber with a core diameter of 9 and 4.4 μm, respectively. The temperature and strain sensitivities of the energy-apodized fiber gratings with a core diameter of 4.4 μm are 10.36 pm/°C and 0.9 pm/με, respectively. The high-reflectivity gratings fabricated by this energy apodization method are expected to be used in high-power narrow-linewidth lasers and wavelength division multiplexing (WDM) systems.
The Study of the Kinetics of Metalaxyl Accumulation and Dissipation in Durian (Durio zibethinus L.) Leaf Using High-Performance Liquid Chromatography (HPLC) Technique
Authors: Phetkhajone Supawadee , Pichakum Aussanee , Songnuan Wisuwat
Published: April 2021

Metalaxyl is an effective approach to control Phytophthora palmivora infection in durian plantation. However, inappropriate metalaxyl usage may increase production cost, pathogen with fungicide resistance, and environmental toxicity. This study established and validated a simple and reproducible procedure to measure metalaxyl concentration in the durian leaf using HPLC. Linearity of the detection ranged from 1–100 µg/mL. The limits of detection (LOD) and quantification (LOQ) were 0.27 and 0.91 µg/mL, respectively. The extraction method gave recovery rates ranging from 88% to 103%. Durian seedlings were treated with 4 g/L metalaxyl either by foliar spray or soil drench. The highest metalaxyl accumulation in durian leaf was found between 6–24 h after treatment and persisted above its effective concentration at least 60 days after foliar application. The dissipation pattern fit to a first-order kinetics equation showed a half-life of 16.50 days. Soil drenching led to eight times higher metalaxyl concentrations in plants than foliar spraying and caused plant death within 15 days after application. These results suggest that foliar spraying of 4 g/L metalaxyl or soil drenching at a lower concentration every two months is sufficient in controlling P. palmivora infection in durian seedlings.
Genome-Wide In Silico Identification and Comparative Analysis of Dof Gene Family in Brassica napus
Authors: Lohani Neeta , Babaei Saeid , Singh Mohan B. , Bhalla Prem L.
Published: April 2021

DNA binding with one finger (DOF) proteins are plant-specific transcription factors that play roles in diverse plant functions. However, little is known about the DOF protein repertoire of the allopolyploid crop, Brassica napus. This in silico study identified 117 Brassica napus Dof genes (BnaDofs) and classified them into nine groups (A, B1, B2, C1, C2.1, C2.2, C3, D1, and D2), based on phylogenetic analysis. Most members belonging to a particular group displayed conserved gene structural organisation and protein motif distribution. Evolutionary analysis exemplified that the divergence of the Brassica genus from Arabidopsis, the whole-genome triplication event, and the hybridisation of Brassica oleracea and Brassica rapa to form B. napus, followed by gene loss and rearrangements, led to the expansion and divergence of the Dof transcription factor (TF) gene family in B. napus. So far, this is the largest number of Dof genes reported in a single eudicot species. Functional annotation of BnaDof proteins, cis-element analysis of their promoters, and transcriptomic analysis suggested potential roles in organ development, the transition from the vegetative to the reproductive stage, light responsiveness, phytohormone responsiveness, as well as potential regulatory roles in abiotic stress. Overall, our results provide a comprehensive understanding of the molecular structure, evolution, and possible functional roles of Dof genes in plant development and abiotic stress response.
Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)
Authors: Mićović Tijana , Topalović Dijana , Živković Lada , Spremo-Potparević Biljana , Jakovljević Vladimir , Matić Sanja , Popović Suzana , Baskić Dejan , Stešević Danijela , Samardžić Stevan , Stojanović Danilo , Maksimović Zoran
Published: April 2021

Hyssopus officinalis L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50 = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable H. officinalis medicinal potential, which calls for further investigation.
Early Growth Stage Characterization and the Biochemical Responses for Salinity Stress in Tomato
Authors: Alam Md Sarowar , Tester Mark , Fiene Gabriele , Mousa Magdi Ali Ahmed
Published: April 2021

Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most susceptible genotype. To investigate the possible biochemical basis for salt stress tolerance, we further characterized six tomato genotypes with varying levels of salinity tolerance. A higher increase in proline content, and antioxidants activities were observed for the salt-tolerant genotypes in comparison to the susceptible genotypes. Salt-tolerant genotypes identified in this work herald a promising source in the tomato improvement program or for grafting as scions with improved salinity tolerance in tomato.
Dissection of Genetic Basis Underpinning Kernel Weight-Related Traits in Common Wheat
Authors: Li Shunda , Wang Liang , Meng Yaning , Hao Yuanfeng , Xu Hongxin , Hao Min , Lan Suque , Zhang Yingjun , Lv Liangjie , Zhang Kai , Peng Xiaohui , Lan Caixia , Li Xingpu , Zhang Yelun
Published: April 2021

Genetic dissection kernel weight-related traits is of great significance for improving wheat yield potential. As one of the three major yield components of wheat, thousand kernel weight (TKW) was mainly affected by grain length (GL) and grain width (GW). To uncover the key loci for these traits, we carried out a quantitative trait loci (QTL) analysis of an F6 recombinant inbred lines (RILs) population derived from a cross of Henong 5290 (small grain) and 06Dn23 (big grain) with a 50 K single nucleotide polymorphism (SNP) array. A total of 17 stable and big effect QTL, including 5 for TKW, 8 for GL and 4 for GW, were detected on the chromosomes 1B, 2A, 2B, 2D, 4B, 5A, 6A and 6D, respectively. Among these, there were two co-located loci for three traits that were mapped on the chromosome 4BS and 6AL. The QTL on 6AL was the most stable locus and explained 15.4–24.8%, 4.1–8.8% and 15.7–24.4% of TKW, GW and GL variance, respectively. In addition, two more major QTL of GL were located on chromosome arm 2BL and 2DL, accounting for 9.7–17.8% and 13.6–19.8% of phenotypic variance, respectively. In this study, we found one novel co-located QTL associated with GL and TKW in 2DL, QGl.haaf-2DL.2/QTkw.haaf-2DL.2, which could explain 13.6–19.8% and 9.8–10.7% phenotypic variance, respectively. Genetic regions and linked markers of these stable QTL will help to further refine mapping of the corresponding loci and marker-assisted selection (MAS) breeding for wheat grain yield potential improvement.
Structural, Electrical and Electrochemical Properties of Glycerolized Biopolymers Based on Chitosan (CS): Methylcellulose (MC) for Energy Storage Application
Authors: Aziz Shujahadeen B. , Asnawi Ahmad S. F. M. , Kadir Mohd Fakhrul Zamani , Alshehri Saad M. , Ahamad Tansir , Yusof Yuhanees M. , Hadi Jihad M.
Published: April 2021

In this work, a pair of biopolymer materials has been used to prepare high ion-conducting electrolytes for energy storage application (ESA). The chitosan:methylcellulose (CS:MC) blend was selected as a host for the ammonium thiocyanate NH4SCN dopant salt. Three different concentrations of glycerol was successfully incorporated as a plasticizer into the CS–MC–NH4SCN electrolyte system. The structural, electrical, and ion transport properties were investigated. The highest conductivity of 2.29 × 10−4 S cm−1 is recorded for the electrolyte incorporated 42 wt.% of plasticizer. The complexation and interaction of polymer electrolyte components are studied using the FTIR spectra. The deconvolution (DVN) of FTIR peaks as a sensitive method was used to calculate ion transport parameters. The percentage of free ions is found to influence the transport parameters of number density (n), ionic mobility (µ), and diffusion coefficient (D). All electrolytes in this work obey the non-Debye behavior. The highest conductivity electrolyte exhibits the dominancy of ions, where the ionic transference number, tion value of (0.976) is near to infinity with a voltage of breakdown of 2.11 V. The fabricated electrochemical double-layer capacitor (EDLC) achieves the highest specific capacitance, Cs of 98.08 F/g at 10 mV/s by using the cyclic voltammetry (CV) technique.
Innovative Polymeric Hybrid Nanocomposites for Application in Photocatalysis
Authors: Cantarella Maria , Impellizzeri Giuliana , Di Mauro Alessandro , Privitera Vittorio , Carroccio Sabrina Carola
Published: April 2021

The immobilization of inorganic nanomaterials on polymeric substrates has been drawing a lot of attention in recent years owing to the extraordinary properties of the as-obtained materials. The hybrid materials, indeed, combine the benefits of the plastic matter such as flexibility, low-cost, mechanical stability and high durability, with them deriving from their inorganic counterparts. In particular, if the inorganic fillers are nanostructured photocatalysts, the originated hybrid systems will be able to utilize the energy delivered by light, catalysing chemical reactions in a sustainable pathway. Most importantly, since the nanofillers can be ad-hoc anchored to the macromolecular structure, their release in the environment will be prevented, thus overcoming one of the main restrictions that impedes their applications on a large scale. In this review, several typologies of hybrid photocatalytic nanomaterials, obtained by using both organic and inorganic semiconductors and realized with different synthetic protocols, were reported and discussed. In the first part of the manuscript, nanocomposites realized by simply blending the TiO2 or ZnO nanomaterials in thermoplastic polymeric matrices are illustrated. Subsequently, the atomic layer deposition (ALD) technique is presented as an excellent method to formulate polymeric nanocomposites. Successively, some examples of polyporphyrins hybrid systems containing graphene, acting as photocatalysts under visible light irradiation, are discussed. Lastly, photocatalytic polymeric nanosponges, with extraordinary adsorption properties, are shown. All the described materials were deeply characterized and their photocatalytic abilities were evaluated by the degradation of several organic water pollutants such as dyes, phenol, pesticides, drugs, and personal care products. The antibacterial performance was also evaluated for selected systems. The relevance of the obtained results is widely overviewed, opening the route for the application of such multifunctional photocatalytic hybrid materials in wastewater remediation.
GFRP Stiffened Plate with Square Cutout under Axial and Out-of-Plane Load
Authors: Sirajudeen Rahima Shabeen , P Alagusundaramoorthy
Published: April 2021

The high-strength-to-weight ratio and corrosion resistance properties of glass-fiber-reinforced polymer (GFRP) composites makes them potentially well-suited for application in ship structures, bridges and off-shore oil platforms. These structures are often formed by stiffened plates and are subjected to axial load and out-of-plane load. Cutouts and openings are provided in the plates for access and maintenance. The main objective of this study was to examine the buckling behavior of GFRP-stiffened composite plates with square cutouts under a combination of axial and out-of-plane load up to failure. Four blade-stiffened composite plates without a cutout and four with square cutout were fabricated with stiffeners as a continuous layup of the flange plate using glass fiber and epoxy resin. The initial geometric imperfections were measured, and plate imperfections (Δx), stiffener imperfections (Δsy) and overall imperfections (Δsx) were calculated from the measurements. All fabricated-stiffened composite plates were tested up to failure. The finite element model was developed in ANSYS software and validated with the experimental results. It was observed that GFRP-stiffened composite plates failed by stiffener compression/stiffener tension mode of failure. The presence of out-of-plane loads and cutouts reduced the axial load carrying capacity of the stiffened composite plates.
All-Solid-State Potentiometric Ion-Sensors Based on Tailored Imprinted Polymers for Pholcodine Determination
Authors: Abd-Rabboh Hisham S. M. , E. Amr Abd El-Galil , Almehizia Abdulrahman A. , Kamel Ayman H.
Published: April 2021

In recent times, the application of the use of ion-selective electrodes has expanded in the field of pharmaceutical analyses due to their distinction from other sensors in their high selectivity and low cost of measurement, in addition to their high measurement sensitivity. Cost-effective, reliable, and robust all-solid-state potentiometric selective electrodes were designed, characterized, and successfully used for pholcodine determination. The design of the sensor device was based on the use of a screen-printed electrode modified with multiwalled carbon nanotubes (MWCNTs) as a solid-contact transducer. Tailored pholcodine (PHO) molecularly imprinted polymers (MIPs) were prepared, characterized, and used as sensory receptors in the presented potentiometric sensing devices. The sensors exhibited a sensitivity of 31.6 ± 0.5 mV/decade (n = 5, R2 = 0.9980) over the linear range of 5.5 × 10−6 M with a detection limit of 2.5 × 10−7 M. Real serum samples in addition to pharmaceutical formulations containing PHO were analyzed, and the results were compared with those obtained by the conventional standard liquid chromatographic approach. The presented analytical device showed an outstanding efficiency for fast, direct, and low-cost assessment of pholcodine levels in different matrices.
A Decision Support Tool for Water Supply System Decentralization via Distribution Network Sectorization
Authors: Vegas Niño Oscar T. , Martínez Alzamora Fernando , Tzatchkov Velitchko G.
Published: April 2021

Many water supply systems, conceived to operate in centralized manner, face difficulties to adapt to dynamic changes, such as population growth, city extension, and industrial development. Decentralization of these systems may be an effective solution. Known techniques for distribution network sectorization design can help to achieve such a goal, but this has not been recognized in the literature. None of those known techniques considers the conversion of a centralized system to a decentralized one. In this paper, two new distinct yet complementary methodologies for water supply system decentralization by distribution network sectorization are proposed and implemented in a software decision support tool freely available on internet. The first methodology identifies the main flow paths from water sources to some strategic nodes and considers the nodes in these paths as new potential sources for dividing the rest of the network. The second methodology sectorizes the network according to the contribution of sources to the consumption at nodes, based on mass balance equations for the transport of a hypothetical conservative constituent in a steady state. Both methods were applied to two real network models. The results obtained were better, for decentralizing the supply, compared to those obtained by other methodologies proposed in the literature.
Animals in Saami Shamanism: Power Animals, Symbols of Art, and Offerings
Authors: Äikäs Tiina , Fonneland Trude
Published: April 2021

In this paper, we study the role of power animals in contemporary Saami shamanism and how past and present are entwined in the presentation of power animals. In the old Saami worldviews, in addition to animals, spirits and sacred rocks (sieidi, SaaN) were also considered to be able to interact with people. Animals were an important part of offering rituals because livelihood and rituals were intertwined. Past “religions” are used as an inspiration for contemporary shamanistic practices, in line with one of late modernity’s core concepts, namely creativity. Present-day shamanistic practices can be described as ritual creativity, and they combine traces of old and new ritual activities. At the shamanistic festival Isogaisa, organized in northern Norway, these different roles of animals and ritual creativity become evident. Here, animals appear as spirit animals, as well as decorative elements on drums and clothes and as performance. In this paper, we combine material culture studies, interview data, and participatory observations in order to reflect the meanings and use of power animals in contemporary spiritual practices. How are traces of the past used in creating contemporary spirituality? How are animals and their artistic presentations entangled in contemporary shamanism?
Navigating Religion Online: Jewish and Muslim Responses to Social Media
Authors: Ferguson Jauhara , Ecklund Elaine Howard , Rothschild Connor
Published: April 2021

Although social media use among religious communities is proliferating, significant gaps remain in our understanding of how religious minorities perceive social media in relation to their faith and community. Thus, we ask how individuals use religion to frame moral attitudes around social media for Jews and Muslims. Specifically, how does social media shape understandings of community? We analyze 52 interviews with Jews and Muslims sampled from Houston and Chicago. We find that Jews and Muslims view social media as a “double-edged sword”—providing opportunities to expand intracommunal ties and access to religious resources, while also diluting the quality of ties and increasing exposure to religious distractions. These findings help us understand what it is about being a religious minority in the US that might shape how individuals engage with social media. Moreover, they suggest that social media may be transforming faith communities in less embodied ways, a topic that is of particular relevance in our pandemic times.
Arctic Sea Ice Freeboard Retrieval from Envisat Altimetry Data
Authors: Zhang Shengkai , Xuan Yue , Li Jiaxing , Geng Tong , Li Xiao , Xiao Feng
Published: April 2021

Arctic sea ice variations are sensitive to Arctic environmental changes and global changes. Freeboard and thickness are two important parameters in sea ice change research. Satellite altimetry can provide long-time and large-scale sea ice monitoring. We estimated the Arctic sea ice freeboard and its variations for the period from 2002 to 2012 from Envisat satellite altimetry data. To remove geoid undulations, we reprocessed the Envisat data using a newly developed mean sea surface (MSS) model, named DTU18. Residuals in the static geoid were removed by using the moving average technique. We then determined the local sea surface height and sea ice freeboard from the Envisat elevation profiles. We validated our freeboard estimates using two radar freeboard products from the European Space Agency (ESA) Climate Change Initiative (CCI) and the Alfred Wegener Institute (AWI), as well as the Operation IceBridge (OIB) sea ice freeboard product. The overall differences between our estimates and the CCI and AWI data were 0.11 ± 0.14 m and 0.12 ± 0.14 m, respectively. Our estimates show good agreement with the three products for areas of freeboard larger than 0.2 m and smaller than 0.3 m. For areas of freeboard larger than 0.3 m, our estimates correlate better with OIB freeboard than with CCI and AWI. The variations in the Arctic sea ice thickness are discussed. The ice freeboard reached its minimum in 2008 during the research period. Sharp decreases were found in the winters of 2005 and 2007.