bluuebunny/crossref_metadata_2025 huggingface dataset:
Black Soldier Fly, Hermetia illucens as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, Lates calcarifer (Bloch, 1790)
Authors: Hender Amanda , Siddik Muhammad , Howieson Janet , Fotedar Ravi
Published: June 2021

A feeding trial was conducted to test the effects of partial replacement of fishmeal (FM) protein and fish oil (FO) with partially defatted black soldier fly, Hermetia illucens insect protein, and oil, respectively, on growth performance, immune response, gut and skin barrier status, and flesh quality in juvenile barramundi. Four isonitrogenous and isocaloric diets used in the study were a control diet based on FM, 30% FM replaced with H. illucens protein (HiP), 30% FO replaced with H. illucens oil (HiO), and both 30% FM and 30% FO replaced with H. illucens protein and oil (HiPO). Diets were fed twice a day to satiety in triplicated groups of barramundi with an initial body weight of 1.74 ± 0.15 g per fish. At the end of the trial, growth and feed utilization indices were found insignificant (p > 0.05) between the test diets and control. A significant increase in bactericidal activity was observed in fish fed the HiP diet while serum lysozyme activity was unchanged. Stress-related heat shock proteins (HSP70 and HSP90) did not differ significantly among the test diets while immune-relevant genes (IL-1β and IL-10) were significantly upregulated in HiP and HiOP groups. The number of mucin cells were increased in the gut and skin of HiP and HiOP fed fish when compared to the control diet. The total fatty acid compositions (∑SFA, ∑MUFA, ∑PUFA, ∑n-3, and ∑n-6) in the muscles of barramundi were not significantly influenced with H. illucens protein and oil diets when compared to the control.
Exosome-Derived MicroRNAs of Human Milk and Their Effects on Infant Health and Development
Authors: Melnik Bodo C. , Stremmel Wolfgang , Weiskirchen Ralf , John Swen Malte , Schmitz Gerd
Published: June 2021

Multiple biologically active components of human milk support infant growth, health and development. Milk provides a wide spectrum of mammary epithelial cell-derived extracellular vesicles (MEVs) for the infant. Although the whole spectrum of MEVs appears to be of functional importance for the growing infant, the majority of recent studies report on the MEV subfraction of milk exosomes (MEX) and their miRNA cargo, which are in the focus of this review. MEX and the dominant miRNA-148a play a key role in intestinal maturation, barrier function and suppression of nuclear factor-κB (NF-κB) signaling and may thus be helpful for the prevention and treatment of necrotizing enterocolitis. MEX and their miRNAs reach the systemic circulation and may impact epigenetic programming of various organs including the liver, thymus, brain, pancreatic islets, beige, brown and white adipose tissue as well as bones. Translational evidence indicates that MEX and their miRNAs control the expression of global cellular regulators such as DNA methyltransferase 1—which is important for the up-regulation of developmental genes including insulin, insulin-like growth factor-1, α-synuclein and forkhead box P3—and receptor-interacting protein 140, which is important for the regulation of multiple nuclear receptors. MEX-derived miRNA-148a and miRNA-30b may stimulate the expression of uncoupling protein 1, the key inducer of thermogenesis converting white into beige/brown adipose tissue. MEX have to be considered as signalosomes derived from the maternal lactation genome emitted to promote growth, maturation, immunological and metabolic programming of the offspring. Deeper insights into milk’s molecular biology allow the conclusion that infants are both “breast-fed” and “breast-programmed”. In this regard, MEX miRNA-deficient artificial formula is not an adequate substitute for breastfeeding, the birthright of all mammals.
The Impact of Hypertension and Atrial Fibrillation on Cognitive Decline and Subclinical Atherosclerosis
Authors: Militaru Marius , Rachieru Ciprian , Lighezan Daniel Florin , Militaru Anda Gabriela
Published: June 2021

Background: Assessment of cognitive impairment and the presence of subclinical atherosclerosis are very important especially in patients with cardiovascular risk factors. Methods: We included 155 hypertensive patients (84 with AF versus 71 without AF) to identify the premature cognitive impairment, the earliest signs of subclinical atherosclerosis and onset of myocardial dysfunction and to evaluate the type of anticoagulation used, the importance of CHA₂DS₂-VASc score (</>3), age (</>65 years) in hypertensive patients with AF. Results: Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment Scale (MoCA), Left Ventricular Ejection Fraction (LVEF) were significantly decreased, and Activities of Daily Living Score (ADL), Geriatric Depression Scale(GDS-15), and intima–media thickness (IMT) were significantly increased in hypertensive patients with AF vs. without AF (p < 0.05). MMSE was significantly decreased, ADL and IMT were significant increased in patients with AF and CHA₂DS₂-VASc>3 and non-vitamin K antagonists oral anticoagulants therapy (NOACs)(p < 0.05). Patients with age >65 with AF had higher rates of cognitive impairment (MMSE significant decrease) and a larger IMT (significant increase) versus patients with AF and age <65 (p < 0.05). Conclusions: Cognitive impairment is encountered in hypertensive patients having AF. Our conclusions suggest a direct link between cognitive impairment, depression, hypertension, AF, age, CHA₂DS₂-VASc score, type of anticoagulants used, LVEF, cognitive parameters, and IMT. We acknowledge the importance of identifying and preventing cognitive changes.
Guidelines to Calibrate a Multi-Residential Building Simulation Model Addressing Overheating Evaluation and Residents’ Influence
Authors: Schünemann Christoph , Schiela David , Ortlepp Regine
Published: June 2021

Can building performance simulation reproduce measured summertime indoor conditions of a multi-residential building in good conformity? This question is answered by calibrating simulated to monitored room temperatures of several rooms of a multi-residential building for an entire summer in two process steps. First, we did a calibration for several days without the residents being present to validate the building physics of the 3D simulation model. Second, the simulations were calibrated for the entire summer period, including the residents’ impact on evolving room temperature and overheating. As a result, a high degree of conformity between simulation and measurement could be achieved for all monitored rooms. The credibility of our results was secured by a detailed sensitivity analysis under varying meteorological conditions, shading situations, and window ventilation or room use in the simulation model. For top floor dwellings, a high overheating intensity was evoked by a combination of insufficient use of night-time window ventilation and non-heat-adapted residential behavior in combination with high solar gains and low heat storage capacities. Finally, the overall findings were merged into a process guideline to describe how a step-by-step calibration of residential building simulation models can be done. This guideline is intended to be a starting point for future discussions about the validity of the simplified boundary conditions which are often used in present-day standard overheating assessment.
Synthesis of Dimethyl Carbonate from CO2 and Methanol over Zr-Based Catalysts with Different Chemical Environments
Authors: Huo Linmeng , Wang Tian , Xuan Keng , Li Lei , Pu Yanfeng , Li Chenxin , Qiao Congzhen , Yang Hao , Bai Yan
Published: June 2021

The adsorption and activation of both CO2 and methanol are mainly affected by the distance of the Lewis acid site, Zr4+, and Lewis base, Zr4+/O2−, of the Zr-based catalysts. In this paper, Zr-incorporated SBA-15 (Zr-SBA-15) and Zr-grafted SBA-15 (Zr/SBA-15) catalysts were prepared with different Zr environments, and were analyzed with N2 adsorption–desorption isotherms, X-ray diffraction, UV-vis spectra, and XPS. It was proposed that Zr-SBA-15 catalyst with Si-O-Zr-OH and Zr-O-Si-OH structure exhibited non-adjacent sites between Zr4+ and Zr4+/O2−, while Zr/SBA-15 catalyst with Zr-O-Zr-OH structure showed neighboring sites between Zr4+ and Zr4+/O2−. Furthermore, the Zr/SBA-15 catalyst exhibited good catalytic activity, while no DMC was detected over the Zr-SBA-15 catalyst at the same reaction conditions. For combined in situ infrared and catalytic performance, it was indicated that the methanol and CO2 could be activated to form DMC, only when the Zr4+ and Zr4+/O2− sites existed and were adjacent to each other in the Zr-O-Zr-OH of Zr/SBA-15 catalyst.
Feasibility of BRCA1/2 Testing of Formalin-Fixed and Paraffin-Embedded Pancreatic Tumor Samples: A Consecutive Clinical Series
Authors: Bruno Rossella , Sensi Elisa , Lupi Cristiana , Giordano Mirella , Bernardini Laura , Vivaldi Caterina , Fornaro Lorenzo , Vasile Enrico , Campani Daniela , Fontanini Gabriella
Published: June 2021

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer, with most patients diagnosed at advanced stages. First-line treatment based on a combined chemotherapy (FOLFIRINOX or gemcitabine plus nab-paclitaxel) provides limited benefits. Olaparib, a PARP inhibitor, has been approved as maintenance for PDAC patients harboring germline BRCA1/2 pathogenic mutations and previously treated with a platinum-based chemotherapy. BRCA1/2 germline testing is recommended, but also somatic mutations could predict responses to PARP inhibitors. Analysis of tumor tissues can detect both germline and somatic mutations and potential resistance alterations. Few data are available about BRCA1/2 testing on pancreatic tumor tissues, which often include limited biological material. We performed BRCA1/2 testing, by an amplicon-based Next Generation Sequencing (NGS) panel, on 37 consecutive PDAC clinical samples: 86.5% of cases were adequate for NGS analysis, with a success rate of 81.2% (median DNA input: 10 nanograms). Three BRCA2 mutations were detected (11.5%). Failed samples were all from tissue macrosections, which had higher fragmented DNA than standard sections, biopsies and fine-needle aspirations, likely due to fixation procedures. BRCA1/2 testing on pancreatic tumor tissues can also be feasible on small biopsies, but more cases must be analyzed to define its role and value in the PDAC diagnostic algorithm.
Active Ratio Test (ART) as a Novel Diagnostic for Ovarian Cancer
Authors: Kang Sung-Woog , Rainczuk Adam , Oehler Martin K. , Jobling Thomas W. , Plebanski Magdalena , Stephens Andrew N.
Published: June 2021

Background: Despite substantial effort, there remains a lack of biomarker-based, clinically relevant testing for the accurate, non-invasive diagnostic or prognostic profiling of epithelial ovarian cancers (EOC). Our previous work demonstrated that whilst the inflammatory marker C-X-C motif chemokine ligand 10 (CXCL10) has prognostic relevance in ovarian cancer, its use is complicated by the presence of multiple, N-terminally modified variants, mediated by several enzymes including Dipeptidyl Peptidase 4 (DPP4). Methods: In this study, we provide the first evidence for the “Active Ratio Test” (ART) as a novel method to measure biologically relevant CXCL10 proteoforms in clinical samples. Results: In a cohort of 275 patients, ART accurately differentiated patients with malignant EOCs from those with benign gynaecological conditions (AUC 0.8617) and significantly out-performed CA125 alone. Moreover, ART combined with the measurement of CA125 and DPP4 significantly increased prognostic performance (AUC 0.9511; sensitivity 90.0%; specificity 91.7%; Cohen’s d > 1) for EOC detection. Conclusion: Our data demonstrate that ART provides a useful method to accurately discriminate between patients with benign versus malignant EOC, and highlights their relevance to ovarian cancer diagnosis. This marker combination may also be applicable in broader screening applications, to identify or discriminate benign from malignant disease in asymptomatic women.
Sentiment Analysis for Fake News Detection
Authors: Alonso Miguel A. , Vilares David , Gómez-Rodríguez Carlos , Vilares Jesús
Published: June 2021

In recent years, we have witnessed a rise in fake news, i.e., provably false pieces of information created with the intention of deception. The dissemination of this type of news poses a serious threat to cohesion and social well-being, since it fosters political polarization and the distrust of people with respect to their leaders. The huge amount of news that is disseminated through social media makes manual verification unfeasible, which has promoted the design and implementation of automatic systems for fake news detection. The creators of fake news use various stylistic tricks to promote the success of their creations, with one of them being to excite the sentiments of the recipients. This has led to sentiment analysis, the part of text analytics in charge of determining the polarity and strength of sentiments expressed in a text, to be used in fake news detection approaches, either as a basis of the system or as a complementary element. In this article, we study the different uses of sentiment analysis in the detection of fake news, with a discussion of the most relevant elements and shortcomings, and the requirements that should be met in the near future, such as multilingualism, explainability, mitigation of biases, or treatment of multimedia elements.
Analyzing and Visualizing Deep Neural Networks for Speech Recognition with Saliency-Adjusted Neuron Activation Profiles
Authors: Krug Andreas , Ebrahimzadeh Maral , Alemann Jost , Johannsmeier Jens , Stober Sebastian
Published: June 2021

Deep Learning-based Automatic Speech Recognition (ASR) models are very successful, but hard to interpret. To gain a better understanding of how Artificial Neural Networks (ANNs) accomplish their tasks, several introspection methods have been proposed. However, established introspection techniques are mostly designed for computer vision tasks and rely on the data being visually interpretable, which limits their usefulness for understanding speech recognition models. To overcome this limitation, we developed a novel neuroscience-inspired technique for visualizing and understanding ANNs, called Saliency-Adjusted Neuron Activation Profiles (SNAPs). SNAPs are a flexible framework to analyze and visualize Deep Neural Networks that does not depend on visually interpretable data. In this work, we demonstrate how to utilize SNAPs for understanding fully-convolutional ASR models. This includes visualizing acoustic concepts learned by the model and the comparative analysis of their representations in the model layers.
Characterization and Study of the Primitive Dynamic Movements Required to Bi-Manipulate a Box
Authors: Hernandez-Vicen Juan , Martinez Santiago , de Santos-Rico Raul , Menendez Elisabeth , Balaguer Carlos
Published: June 2021

Automating the action of finding the opening side of a box is not possible if the robot is not capable of reaching and evaluating all of its sides. To achieve this goal, in this paper, three different movement strategies to bi-manipulate a box are studied: overturning, lifting, and spinning it over a surface. First of all, the dynamics involved in each of the three movement strategies are studied using physics equations. Then, a set of experiments are conducted to determine if the real response of the humanoid robot, TEO, to a box is consistent with the expected answer based on theoretical calculus. After the dynamics validation, the information on the forces and the position in the end effectors is used to characterize these movements and create its primitives. These primitive movements will be used in the future to design a hybrid position–force control in order to adapt the movements to different kinds of boxes. The structure of this control is also presented in this paper.
All-Dielectric Metasurface for Sensing Microcystin-LR
Authors: Ma Binze , Ouyang Ao , Zhong Juechen , Belov Pavel A. , Sinha Ravindra Kumar , Qian Weiping , Ghosh Pintu , Li Qiang
Published: June 2021

Sensing Microcystin-LR (MC-LR) is an important issue for environmental monitoring, as the MC-LR is a common toxic pollutant found in freshwater bodies. The demand for sensitive detection method of MC-LR at low concentrations can be addressed by metasurface-based sensors, which are feasible and highly efficient. Here, we demonstrate an all-dielectric metasurface for sensing MC-LR. Its working principle is based on quasi-bound states in the continuum mode (QBIC), and it manifests a high-quality factor and high sensitivity. The dielectric metasurface can detect a small change in the refractive index of the surrounding environment with a quality factor of ~170 and a sensitivity of ~788 nm/RIU. MC-LR can be specifically identified in mixed water with a concentration limit of as low as 0.002 μg/L by a specific recognition technique for combined antigen and antibody. Furthermore, the demonstrated detection of MC-LR can be extended to the identification and monitoring of other analytes, such as viruses, and the designed dielectric metasurface can serve as a monitor platform with high sensitivity and high specific recognition capability.
Intelligent Security Monitoring System Based on RISC-V SoC
Authors: Wu Wenjuan , Su Dongchu , Yuan Bo , Li Yong
Published: June 2021

With the development of the economy and society, the demand for social security and stability increases. However, traditional security systems rely too much on human resources and are affected by uncontrollable community security factors. An intelligent security monitoring system can overcome the limitations of traditional systems and save human resources, contributing to public security. To build this system, a RISC-V SoC is first designed in this paper and implemented on the Nexys-Video Artix-7 FPGA. Then, the Linux operating system is transplanted and successfully run. Meanwhile, the driver of related hardware devices is designed independently. After that, three OpenCV-based object detection models including YOLO (You Only Look Once), Haar (Haar-like features), and LBP (Local Binary Pattern) are compared, and the LBP model is chosen to design applications. Finally, the processing speed of 1.25 s per frame is realized to detect and track moving objects. To sum up, we build an intelligent security monitoring system with real-time detection, tracking, and identification functions through hardware and software collaborative design. This paper also proposes a video downsampling technique. Based on this technique, the BRAM resource usage on the hardware side is reduced by 50% and the amount of pixel data that needs to be processed on the software side is reduced by 75%. A video downsampling technology is also proposed in this paper to achieve better video display effects under limited hardware resources. It provides conditions for future function expansion and improves the models’ processing speed. Additionally, it reduces the run time of the application and improves the system performance.
Low-Cost Electrodeposition of Size-Tunable Single-Crystal ZnO Nanorods
Authors: Sakellis Elias , Markopoulos Antonis , Tzouvelekis Christos , Chatzigeorgiou Manolis , Travlos Anastasios , Boukos Nikos
Published: June 2021

In this paper we report a low cost, simple, electrochemical method for large-area growth of single crystal ZnO nanorods. The method utilizes a metallic zinc foil as the source of the necessary zinc ions for ZnO growth on indium-doped tin oxide (ITO) glass slides. The method is thoroughly discussed and investigated varying all the parameters involved. The resulting ZnO nanorods are highly oriented along c-axis and densely packed, while their length and diameter can be tuned by varying the growth parameters. Two different types of seed layers on the ITO glass slides are tested. A seed layer made by spin coating of ZnO nanoparticles results in a twofold increase of the ZnO nanorod surface density as compared with a ZnO thin film seed layer by physical vapor deposition. Additionally, the effect of oxygen supply during electrodeposition was investigated as a crucial regulatory parameter not only for the geometrical and topological characteristics of the ZnO nano-arrays but for their physical properties as well.
Oat-Based Foods: Chemical Constituents, Glycemic Index, and the Effect of Processing
Authors: Zhang Kailong , Dong Rui , Hu Xinzhong , Ren Changzhong , Li Yuwei
Published: June 2021

The desire for foods with lower glycemic indices has led to the exploration of functional ingredients and novel food processing techniques. The glycemic index (GI) is a well-recognized tool to assess the capacity of foods to raise blood glucose levels. Among cereal crops, oats have shown the greatest promise for mitigating glycemic response. This review evaluated decades of research on the effects of oat components on the GI level of oat-based foods with specific emphasis on oat starch, β-glucans, proteins, and phenolics. The effects of commonly used processing techniques in oats on GI level, including heating, cooling, and germination were also discussed. In addition, the GI of oat-based foods in various physical formats such as whole grain, flakes, and flour was systematically summarized. The aim of this review was to synthesize knowledge of the field and to provide a deeper understanding of how the chemical composition and processing of oats affect GI, thereby further benefiting the development of low-GI oat foods.
SyPEAH: The WebAPP System for Protection and Education to Archaeological Heritage in the Parco Archeologico del Colosseo
Authors: Giovampaola Irma Della
Published: June 2021

Archaeological sites are affected by changes due to a natural deterioration process over time. If not prevented, this may compromise the functionality of the cultural property, and in turn become pathological and result in degradation. Monitoring through innovative technologies paves the way towards an effective planned maintenance activity and therefore preventive conservation. The monitoring project of the Parco Archeologico del Colosseo was inspired by the desire to build a system of protection and conservation at the service of sustainable exploitation. Established by Ministerial Decree 12 January 2017 in art. 3, the park is an independent cultural site of the Ministry of Culture. It includes the central area of Rome—the Roman Forum, the Palatine, the Colosseum and the Domus Aurea—and has an extension of about 77 hectares, of which about 32 are buildings. With these objectives, the Parco Archeologico del Colosseo has launched a static and dynamic monitoring project consisting of six fundamental levels of activities. The project involves the creation of a multi-parameter system of permanent control of the entire archaeological area, with the associated indicators of the level of risk, for which it is necessary the combined use of innovative technologies.
Reference Evapotranspiration (ETo) Methods Implemented as ArcMap Models with Remote-Sensed and Ground-Based Inputs, Examined along with MODIS ET, for Peloponnese, Greece
Authors: Dimitriadou Stavroula , Nikolakopoulos Konstantinos G.
Published: June 2021

The present study develops ArcMap models to implement the following three methods: FAO-56 Penman–Monteith (FAO PM), Hargreaves–Samani (HS) and Hansen, with the former used as a reference. Moreover, three models implementing statistical indices (RMSD, MB, NMB) are also created. The purpose is threefold, as follows: to investigate the variability in the daily mean reference evapotranspiration (ETo) for the Decembers and Augusts during 2016–2019, over Peloponnese, Greece. Furthermore, to investigate the agreement between the methods’ ETo estimates, and examine the former along with MODIS ET (daily) averaged products. The study area is a complex Mediterranean area. Meteorological data from sixty-two stations under the National Observatory of Athens (NOA), and MODIS Terra LST products, have been employed. FAO PM is found sensitive to wind speed and depicts interactions among climate parameters (T, evaporative demand and water availability) in the frame of climate change. The years 2016–2019 are four of the warmest since the preindustrial era. Hargreaves–Samani’s estimations for the Decembers of 2016–2019 were almost identical to MODIS ET, despite their different physical meaning. However, for the Augusts there are considerable discrepancies between the methods’ and MODIS’s estimates, attributed to the higher evaporative demand in the summertime. The GIS models are accurate, reliable, time-saving, and adjustable to any study area.
Obesity and Post-Transplant Diabetes Mellitus in Kidney Transplantation
Authors: Martin-Moreno Paloma Leticia , Shin Ho-Sik , Chandraker Anil
Published: June 2021

Worldwide, the prevalence obesity, diabetes, and chronic kidney disease is increasing apace. The relationship between obesity and chronic kidney disease is multidimensional, especially when diabetes is also considered. The optimal treatment of patients with chronic kidney disease includes the need to consider weight loss as part of the treatment. The exact relationship between obesity and kidney function before and after transplantation is not as clear as previously imagined. Historically, patients with obesity had worse outcomes following kidney transplantation and weight loss before surgery was encouraged. However, recent studies have found less of a correlation between obesity and transplant outcomes. Transplantation itself is also a risk factor for developing diabetes, a condition known as post-transplant diabetes mellitus, and is related to the use of immunosuppressive medications and weight gain following transplantation. Newer classes of anti-diabetic medications, namely SGLT-2 inhibitors and GLP-1 agonists, are increasingly being recognized, not only for their ability to control diabetes, but also for their cardio and renoprotective effects. This article reviews the current state of knowledge on the management of obesity and post-transplant diabetes mellitus for kidney transplant patients.
Does the Addition of Pain Neurophysiology Education to a Therapeutic Exercise Program Improve Physical Function in Women with Fibromyalgia Syndrome? Secondary Analysis of a Randomized Controlled Trial
Authors: Ceballos-Laita Luis , Mingo-Gómez María Teresa , Estébanez-de-Miguel Elena , Bueno-Gracia Elena , Navas-Cámara Francisto José , Verde-Rello Zoraida , Fernández-Araque Ana , Jiménez-del-Barrio Sandra
Published: June 2021

Therapeutic exercise (TE) is one of the most investigated approaches for the management of FMS. Pain neurophysiology education (PNE) helps toward understanding the pain condition, leading to maladaptive pain cognitions and coping strategies in patients with chronic pain. Our study aimed to assess the effects of therapeutic exercise and pain neurophysiology education versus TE in isolation on fatigue, sleep disturbances, and physical function in the short term and at three months of follow-up in women with fibromyalgia syndrome (FMS). A single-blind randomized controlled trial was carried out. A total of 32 women with FMS referred from medical doctors and fibromyalgia association were randomized in 2 groups: PNE + TE group or TE group. Fatigue and sleep disturbances (Visual Analog Score) and physical function (Senior Fitness Test) were assessed before, after intervention, and at three months of follow-up. Significant improvements were achieved in the Timed Up and Go test (p = 0.042) and Arm Curl test (p = 0.043) after intervention and on handgrip in the non-dominant side at three months of follow-up (p = 0.036) on the PNE + TE group. No between-groups differences were found for fatigue, sleep disturbances, and the rest of test included in the Senior Fitness Test. In conclusion, these results suggest that PNE + TE appears to be more effective than TE in isolation for the improvement of physical function (Timed Up and Go test and Arm Curl test) in women with FMS in the short term.
Chronic Liver Disease Increases Mortality Following Pancreatoduodenectomy
Authors: Enderes Jana , Teschke Jessica , Manekeller Steffen , Vilz Tim O. , Kalff Jörg C. , Glowka Tim R.
Published: June 2021

According to the International Study Group of Pancreatic Surgery (ISGPS), data about the impact of pre-existing liver pathologies on delayed gastric emptying (DGE) after pancreatoduodenectomy (PD) according to the definitions of the International Study Group of Pancreatic Surgery (ISGPS) are lacking. We therefore investigated the impact of DGE after PD according to ISGPS in patients with liver cirrhosis (LC) and advanced liver fibrosis (LF). Patients were analyzed with respect to pre-existing liver pathologies (LC and advanced LF, n = 15, 6% vs. no liver pathologies, n = 240, 94%) in relation to demographic factors, comorbidities, intraoperative characteristics, mortality and postoperative complications, with special emphasis on DGE. DGE was equally distributed (DGE grade A, p = 1.000; B, p = 0.396; C, p = 0.607). Particularly, the first day of solid food intake (p = 0.901), the duration of intraoperative administered nasogastric tube (NGT) (p = 0.812), the rate of re-insertion of NGT (p = 0.072), and the need for parenteral nutrition (p = 0.643) did not differ. However, patients with LC and advanced LF showed a higher ASA (American Society of Anesthesiologists) score (p = 0.016), intraoperatively received more erythrocyte transfusions (p = 0.029), stayed longer in the intensive care unit (p = 0.010) and showed more intraabdominal abscess formation (p = 0.006). Moreover, we did observe a higher mortality rate amongst patients with pre-existing liver diseases (p = 0.021), and reoperation was a risk factor for higher mortality (p ≤ 0.001) in the multivariate analysis. In our study, we could not detect a difference with respect to DGE classified by ISGPS; however, we did observe a higher mortality rate amongst these patients and thus, they should be critically evaluated for PD.
Changes in Cerebral Blood Flow after Erenumab Treatment in Good and Non-Responders—A Pilot Study of Migraine Patients
Authors: Nowaczewska Magdalena , Straburzyński Marcin , Meder Grzegorz , Kaźmierczak Wojciech
Published: June 2021

Erenumab showed efficacy in migraine prevention, however we cannot identify which patients to treat by predicting efficacy response. The aim of this study was to compare changes in cerebral blood flow (CBF) reflected by transcranial Doppler (TCD) in erenumab good responders (GR) and non-responders, in order to identify a parameter that could predict the treatment response. In this study, migraineurs treated with erenumab underwent clinical and TCD evaluations before and 6 weeks after the treatment, including data on migraine type, monthly migraine days (MMD), medication overuse headache (MOH) presence, mean blood flow velocity (Vm) and pulsatility index (PI) in cerebral arteries (CA). GR were defined as reporting ≥50% reduction in MMD. Thirty women were enrolled, of mean age 40.53 years, 20 with chronic migraine, 14 with MOH, and 19 were GR. Baseline Vm values in right CA and basilar artery (BA) were significantly lower in GR as compared with non-responders. Vm values in all arteries significantly increased after the treatment as compared with corresponding baseline values, but only in GR. A significant negative correlation was observed between baseline Vm in right CA and treatment effectiveness. Baseline Vm in right CA and basilar artery is reduced in erenumab GR as compared with non-responders. This asymmetry normalizes after the treatment with significant Vm increase in CA which may reflect CBF increase in GR only. Lower baseline Vm in right CA may predict erenumab efficacy; however, these results should be replicated in a larger cohort.
Precision Medicine in Oncology: A Review of Multi-Tumor Actionable Molecular Targets with an Emphasis on Non-Small Cell Lung Cancer
Authors: Stein Matthew K. , Oluoha Oluchukwu , Patel Kruti , VanderWalde Ari
Published: June 2021

Precision medicine is essential for the modern care of a patient with cancer. Comprehensive molecular profiling of the tumor itself is necessary to determine the presence or absence of certain targetable abnormalities or biomarkers. In particular, lung cancer is a disease for which targetable genomic alterations will soon guide therapy in the majority of cases. In this comprehensive review of solid tumor-based biomarkers, we describe the genomic alterations for which targeted agents have been approved by the United States Food and Drug Administration (FDA). While focusing on alterations leading to approvals in a tumor-agnostic fashion (MSI-h, TMB-h, NTRK) and on those alterations with approvals in multiple malignancies (BRAF, ERBB2, RET, BRCA, PD-L1), we also describe several biomarkers or indications that are likely to lead to an approved drug in the near future (e.g., KRAS G12C, PD-L1 amplification, HER2 overexpression in colon cancer, HER2 mutations in lung cancer). Finally, we detail the current landscape of additional actionable alterations (EGFR, ALK, ROS1, MET) in lung cancer, a biomarker-rich malignancy that has greatly benefitted from the precision oncology revolution.
The Effect of Venoactive Drug Therapy on the Development and Severity of Post-Embolization Syndrome in Endovascular Interventions on the Gonadal Veins
Authors: Gavrilov Sergey Gennadievich , Krasavin Gennady Vladimirovich , Mishakina Nadezhda Yurievna , Efremova Oksana Igorevna , Zolotukhin Igor Anatolievich
Published: June 2021

Objective. To evaluate the incidence of post-embolization syndrome (PES) and the effect of venoactive therapy on its development, severity, and duration after endovascular embolization of gonadal veins (EEGV) with coils in patients with pelvic congestion syndrome (PCS). Materials and Methods. We analyzed the outcomes of EEGV with coils in 70 female patients who received (n = 38; group 1) or did not receive (n = 32; group 2) treatment with a venoactive drug (VAD) before and after the procedure. Assessments of the EEGV efficacy and for possible signs of PES were done on days 1, 5, 10, 15, 20, and 30 after the intervention. All patients underwent transvaginal and transabdominal duplex ultrasound scanning (DUS) after EEGV. In addition, patients with PES were examined using the computed tomography of the pelvic veins in the postprocedural period. Results. Technical success of EEGV was achieved in 100% of patients. Pelvic venous pain (PVP) reduction after EEGV was observed in 77.1% of patients. The PES was diagnosed in 18.6% of cases (10.5% in group 1 vs. 28.1% in group 2, p > 0.05). In three patients of group 1, the protrusion of coils was suspected and eventually verified during the resection of the left gonadal vein with coils. The group 1 patients had less severe post-embolization pain (6.2 ± 0.4 vs. 7.8 ± 0.3 scores in group 2; p = 0.009) and three times shorter duration of PES (5.0 ± 1.2 vs. 16.2 ± 2.7 days; p = 0.003). No significant differences in the diameters of gonadal veins, side of embolization, and number of coils were revealed between patients with and without PES. The rate of parametrium vein thrombosis was found to be significantly higher in patients with PES than in those without PES (30.7% vs. 18.5%, respectively; p < 0.05). Conclusion. The PES is a frequent complication of EEGV with coils and occurs in 18.6% of patients. Venoactive treatment does not effect the incidence of this complication but reduces the PES severity and duration.
Multi-Scenario Simulation for the Consequence of Urban Expansion on Carbon Storage: A Comparative Study in Central Asian Republics
Authors: Chen Yang , Yue Wenze , Liu Xue , Zhang Linlin , Chen Ye’an
Published: June 2021

There is growing concern about the consequences of future urban expansion on carbon storage as our planet experiences rapid urbanization. While an increasing body of literature was focused on quantifying the carbon storage impact of future urban expansion across the globe, rare attempts were made from the comparative perspective on the same scale, particularly in Central Asia. In this study, Central Asian capitals, namely Ashkhabad, Bishkek, Dushanbe, Nur Sultan, and Tashkent, were used as cases. According to the potential impacts of BRI (Belt and Road Initiative) on urban expansion, baseline development scenario (BDS), cropland protection scenario (CPS), and ecological protection scenario (EPS) were defined. We then simulated the carbon storage impacts of urban expansion from 2019 to 2029 by using Google Earth Engine, the Future Land Use Simulation model, and the Integrated Valuation of Environmental Services and Tradeoffs model. We further explored the drivers for carbon storage impacts of future urban expansion in five capitals. The results reveal that Nur Sultan will experience carbon storage growth from 2019 to 2029 under all scenarios, while Ashkhabad, Bishkek, Dushanbe, and Tashkent will show a decreasing tendency. EPS and CPS will preserve the most carbon storage for Nur Sultan and the other four cities, respectively. The negative impact of future urban expansion on carbon storage will be evident in Ashkhabad, Bishkek, Dushanbe, and Tashkent, which will be relatively inapparent in Nur Sultan. The potential drivers for carbon storage consequences of future urban expansion include agricultural development in Bishkek, Dushanbe, and Tashkent, desert city development in Ashkhabad, and prioritized development of the central city and green development in Nur Sultan. We suggest that future urban development strategies for five capitals should be on the basis of differentiated characteristics and drivers for the carbon storage impacts of future urban expansion.
Strategies to Improve Antimicrobial Utilization with a Special Focus on Developing Countries
Authors: Godman Brian , Egwuenu Abiodun , Haque Mainul , Malande Oliver Ombeva , Schellack Natalie , Kumar Santosh , Saleem Zikria , Sneddon Jacqueline , Hoxha Iris , Islam Salequl , Mwita Julius , do Nascimento Renata Cristina Rezende Macedo , Dias Godói Isabella Piassi , Niba Loveline Lum , Amu Adefolarin A. , Acolatse Joseph , Incoom Robert , Sefah Israel Abebrese , Opanga Sylvia , Kurdi Amanj , Chikowe Ibrahim , Khuluza Felix , Kibuule Dan , Ogunleye Olayinka O. , Olalekan Adesola , Markovic-Pekovic Vanda , Meyer Johanna C. , Alfadl Abubakr , Phuong Thuy Nguyen Thi , Kalungia Aubrey C. , Campbell Stephen , Pisana Alice , Wale Janney , Seaton R. Andrew
Published: June 2021

Antimicrobial resistance (AMR) is a high priority across countries as it increases morbidity, mortality and costs. Concerns with AMR have resulted in multiple initiatives internationally, nationally and regionally to enhance appropriate antibiotic utilization across sectors to reduce AMR, with the overuse of antibiotics exacerbated by the COVID-19 pandemic. Effectively tackling AMR is crucial for all countries. Principally a narrative review of ongoing activities across sectors was undertaken to improve antimicrobial use and address issues with vaccines including COVID-19. Point prevalence surveys have been successful in hospitals to identify areas for quality improvement programs, principally centering on antimicrobial stewardship programs. These include reducing prolonged antibiotic use to prevent surgical site infections. Multiple activities centering on education have been successful in reducing inappropriate prescribing and dispensing of antimicrobials in ambulatory care for essentially viral infections such as acute respiratory infections. It is imperative to develop new quality indicators for ambulatory care given current concerns, and instigate programs with clear public health messaging to reduce misinformation, essential for pandemics. Regular access to effective treatments is needed to reduce resistance to treatments for HIV, malaria and tuberculosis. Key stakeholder groups can instigate multiple initiatives to reduce AMR. These need to be followed up.
Comparative EIS Study of AlxCoCrFeNi Alloys in Ringer’s Solution for Medical Instruments
Authors: Socorro-Perdomo Pedro P. , Florido-Suárez Néstor R. , Voiculescu Ionelia , Mirza-Rosca Julia C.
Published: June 2021

Depending on the properties required for the medical instruments, compared with the classical materials, the high-entropy alloys (HEAs) are a versatile option. Electrochemical Impedance Spectroscopy (EIS) measurements have been performed on AlxCoCrFeNi-type high-entropy alloys with various concentrations of Al content (x = 0.6, 0.8, and 1.0) in order to characterize their passive film and corrosion resistance at 37 °C under infectious simulated physiological conditions (Ringer´s solution acidulated with HCl) at pH = 3. The impedance spectra were obtained at different potential values between −0.7 and +0.7 V vs. SCE. Analysis of the impedance spectra was carried out by fitting different equivalent circuits to the experimental data. Two equivalent circuits, with one time constant and two time constants respectively, can be satisfactorily used for fitting the spectra: one time constant represents the characteristics of the compact passive film, and the second one is for the porous passive film. With the decreasing of Al content, the obtained EIS results are correlated with the evolution of the microhardness and microstructure, which is characterized by Optical Microscopy (OM), Scanning Electron Microscopy (SEM), and Energy-Dispersive X-Ray Spectroscopy (EDAX). It can be observed for all alloys that the resistance of the passive film is very high and decreases with the potential: the very high resistance of the passive film implies a high corrosion resistance, which can be assigned to the formation of the protective oxide layer and demonstrates that the analyzed alloys fulfill the prerequisites for their use as new materials for the manufacturing of medical instruments.
Application of Commercial Surface Pretreatments on the Formation of Cerium Conversion Coating (CeCC) over High-Strength Aluminum Alloys 2024-T3 and 7075-T6
Authors: Alba-Galvín Juan Jesús , González-Rovira Leandro , Botana Francisco Javier , Lekka Maria , Andreatta Francesco , Fedrizzi Lorenzo , Bethencourt Manuel
Published: June 2021

The selection of appropriate surface pretreatments is one of the pending issues for the industrial application of cerium-based chemical conversion coatings (CeCC) as an alternative for toxic chromate conversion coating (CrCC). A two-step surface pretreatment based on commercial products has been successfully used here to obtain CeCC on AA2024-T3 and AA7075-T6. Specimens processed for 1 to 15 min in solutions containing CeCl3 and H2O2 have been studied by scanning electron microscopy coupled with energy-dispersive X-ray analysis (SEM-EDX), glow discharge optical emission spectroscopy (GDOES), potentiodynamic linear polarization (LP), electrochemical impedance spectroscopy (EIS), and neutral salt spray (NSS) tests. SEM-EDX showed that CeCC was firstly observed as deposits, followed by a general coverage of the surface with the formation of cracks where the coating was getting thicker. GDOES confirmed an increase of the CeCC thickness as the deposition proceed, the formation of CeCC over 7075 being faster than over 2024. There was a Ce-rich layer in both alloys and an aluminum oxide/hydroxide layer on 7075 between the upper Ce-rich layer and the aluminum matrix. According to LP and EIS, CeCC in all samples offered cathodic protection and comparable degradation in chloride-containing media. Finally, the NSS test corroborated the anti-corrosion properties of the CeCC obtained after the commercial pretreatments employed.
Review on Bee Products as Potential Protective and Therapeutic Agents in Male Reproductive Impairment
Authors: Suleiman Joseph Bagi , Bakar Ainul Bahiyah Abu , Mohamed Mahaneem
Published: June 2021

Bee products are sources of functional food that have been used in complementary medicine to treat a variety of acute and chronic illnesses in many parts of the world. The products vary from location to location as well as country to country. Therefore, the aim of this review was to identify various bee products with potential preventive and therapeutic values used in the treatment of male reproductive impairment. We undertook a vigorous search for bee products with preventive and therapeutic values for the male reproductive system. These products included honey, royal jelly, bee pollen, bee brood, apilarnil, bee bread, bee wax, and bee venom. We also explained the mechanisms involved in testicular steroidogenesis, reactive oxygen species, oxidative stress, inflammation, and apoptosis, which may cumulatively lead to male reproductive impairment. The effects of bee pollen, bee venom, honey, propolis, royal jelly, and bee bread on male reproductive parameters were examined. Conclusively, these bee products showed positive effects on the steroidogenic, spermatogenic, oxidative stress, inflammatory, and apoptotic parameters, thereby making them a promising possible preventive and therapeutic treatment of male sub/infertility.
Pyrazoles and Pyrazolines as Anti-Inflammatory Agents
Authors: Mantzanidou Martha , Pontiki Eleni , Hadjipavlou-Litina Dimitra
Published: June 2021

The five-membered heterocyclic group of pyrazoles/pyrazolines plays important role in drug discovery. Pyrazoles and pyrazolines present a wide range of biological activities. The synthesis of the pyrazolines and pyrazole derivatives was accomplished via the condensation of the appropriate substituted aldehydes and acetophenones, suitable chalcones and hydrazine hydrate in absolute ethanol in the presence of drops of glacial acetic acid. The compounds are obtained in good yields 68–99% and their structure was confirmed using IR, 1H-NMR, 13C-NMR and elemental analysis. The novel derivatives were studied in vitro for their antioxidant, anti-lipid peroxidation (AAPH) activities and inhibitory activity of lipoxygenase. Both classes strongly inhibit lipid peroxidation. Compound 2g was the most potent lipoxygenase inhibitor (IC50 = 80 µM). The inhibition of the carrageenin-induced paw edema (CPE) and nociception was also determined, with compounds 2d and 2e being the most potent. Compound 2e inhibited nociception higher than 2d. Pyrazoline 2d was found to be active in a preliminary test, for the investigation of anti-adjuvant-induced disease (AID) activity. Pyrazoline derivatives were found to be more potent than pyrazoles. Docking studies of the most potent LOX inhibitor 2g highlight hydrophobic interactions with VAL126, PHE143, VAL520 and LYS526 and a halogen bond between the chlorine atom and ARG182.
Cerium/Ascorbic Acid/Iodine Active Species for Redox Flow Energy Storage Battery
Authors: Chang Tzu-Chin , Liu Yu-Hsuan , Chen Mei-Ling , Tseng Chen-Chen , Lin Yung-Sheng , Huang Shu-Ling
Published: June 2021

In this study, we developed a novel cerium/ascorbic acid/iodine active species to design a redox flow battery (RFB), in which the cerium nitrate hexahydrate [Ce(NO3)3·6H2O] was used as a positive Ce3+/Ce4+ ion pair, and the potassium iodate (KIO3) containing ascorbic acid was used as a negative I2/I− ion pair. In order to improve the electrochemical activity and to avoid cross-contamination of the redox pair ions, the electroless plating and sol–gel method were applied to modify the carbon paper electrode and the Nafion 117 membrane. The electrocatalytic and electrochemical properties of the composite electrode using methanesulfonic acid as a supporting electrolyte were assessed using the cyclic voltammetry (CV) test. The results showed that the Ce (III)/Ce (IV) active species presented a symmetric oxidation/reduction current ratio (1.09) on the C–TiO2–PdO composite electrode. Adding a constant amount of ascorbic acid to the iodine solution led to a good reversible oxidation/reduction reaction. Therefore, a novel Ce/ascorbic acid/I RFB was developed with C–TiO2–PdO composite electrodes and modified Nafion 117–SiO2–SO3H membrane using the staggered-type flow channel, of which the energy efficiency (EE%) can reach about 72%. The Ce/ascorbic acid/I active species can greatly reduce the electrolyte cost compared to the all-vanadium redox flow battery system, and it therefore has greater development potential.