bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative analysis of the genes affecting development of the hypopharyngeal gland in honey bees (Apis mellifera L.)
Authors: Liu Hao , Wang Zi-Long , Zhou Lin-Bin , Zeng Zhijiang
Published: January 2016

Royal jelly has many important biological functions, however the molecular mechanism of royal jelly secretion in hypopharyngeal gland (HG) is still not well understood. In our previously study, six genes (SV2C, eIF-4E, PDK1, IMP, cell growth-regulating nucleolar protein and TGF-βR1) have been shown to might be associated with royal jelly secretion. In this study, the relative expression levels of these genes were examined in the hypopharyngeal gland of workers at different developmental stages (nurse, forager and reversed nurse stages). The results indicated that the relative expression levels of SV2C, eIF-4E, IMP, cell growth-regulating nucleolar protein and TGF-βR1 were reversed at reversed nurse stage compared to forager stage. We concluded that these genes are possibly candidates related to hypopharyngeal gland development or royal jelly secretion.
Cryptic Lineages in the Cardiocondyla sl. kagutsuchi Terayama (Hymenoptera: Formicidae) Discovered by Phylogenetic and Morphological Approaches
Authors: Okita Ichiro , Terayama Mamoru , Tsuchida Koji
Published: January 2016

The taxonomy of ant species in the genus Cardiocondyla is very confused due to the extreme difficulty in separating many species based on morphology alone. In Japan, one group of the species complex Cardiocondyla sl. kagutsuchi has both winged and wingless worker-like (ergatoid) males (dimorphic) whereas others have only ergatoid males (monomorphic). The presence of both groups prompted us to hypothesize that C. sl. kagutsuchi presumably includes several independent species with differences in their male wing morphologies. However, whether any species boundary actually exists between the male groups has remained unsolved over the 10+ years since the previous revision of this genus. In this study, using discriminant and phylogenetic analyses, we compared the worker caste morphology of this species complex among lineages detected by phylogenetic analyses. In addition, we examined the number of sexuals present in field colonies. Our results revealed the existence of at least three morphological and phylogenetic groups within this species complex.
Mediatised local government: social media activity and media strategies among local government officials 1989–2010
Authors: Djerf-Pierre Monika , Pierre Jon
Published: January 2016

The article examines how local government officials in Sweden use social media and to what extent the emergence of social media has altered the relationship to conventional news media. The article examines the development of local government-media relations across time on the basis of a unique survey-based data set comparing the local political and administrative leadership’s media strategies in 1989 and 2010. The 2010 survey also included questions on how local officials in Sweden use social media in their work, that is, Facebook, Twitter and blogs. The results show that local officials have appropriated social media in their work, but only to a moderate extent. Local officials engage in social media if and when the local government becomes the target of social media scrutiny. Our study also demonstrates that social media have not replaced conventional media as a means of communication with constituencies. Indeed, officials who are active social media users have more contacts with conventional media compared to less active officials. Social media thus contribute to an intensification of the mediatisation of local governance rather than replacing conventional media in local political communication.
Application of vertical electrical sounding (VES) for groundwater exploration in Onitsha and environs, Nigeria
Authors: Osele Chika , Onwuemesi Ajana , Anakwuba Emmanuel , Chinwuko Augustine
Published: January 2016

Surface geo-electrical survey using vertical electrical sounding (VES) method has been carried out in Onitsha and environs in southwestern part of Anambra state in order to determine the aquifer characteristics and groundwater potential of the area. Eleven vertical electrical soundings were carried out within the area of study using schlumberger array configuration. The interpretation of the vertical electrical sounding (VES) data revealed three to five geoelectric units with depth to the aquiferous layers ranging from 21 to 78m and resistivity of the saturated layers varying between 20 and 5600 ohm-m. Aquifer characteristics such as transmissivity and hydraulic conductivity calculated from interpretated VES result ranged from 2.55m2/day to 29.01m2/day and 0.03m/day to 1.37m/day respectively. This result shows that the water saturated sandstone units of the study area is hydrological good and capable of producing optimum groundwater yield. Furthermore, borehole could be drilled at depth between 40 and 110m in the area for sustainable water supply and hydrochemical study carried out to determine the water quality for domestics and municipal purposes.
Heterozygous mutations in RelB can be associated with immune dysregulation and lymphoma
Authors: Haynes Alison , Kamra Palinder , Roifman Chaim
Published: January 2016

Background: The nuclear factor kappa-B (NFκB) family of transcription factors is essential for numerous processes, including the development and function of the innate and adaptive immune response, inflammation and cell growth, differentiation, and survival. Recently, patients with homozygous mutations in the gene for the NFκB transcription factor RelB have been described as presenting with features of combined immunodeficiency such as recurrent infection and failure to thrive as well as reduced response to mitogens and an inability to maintain an adequate antibody response to immunizations.

Methods: The immune status and genetics of the parents of patients with homozygous RelB mutations were assessed. In vitro mitogen stimulation, flow cytometry, and cytokine ELISA were used to assess immunological status and signal transduction pathways.

Results: Four patients were confirmed to have heterozygous RelB mutations. The majority of patients had evidence of immune dysfunction with impaired in vitro responses to PHA and antigens. One patient developed lymphoma.

Conclusion: Heterozygous RelB mutations can be associated with immune dysregulation with impaired mitogen and antigen responses and lymphoma. It is likely that the immune defects apparent in RelB deficient humans are due to a wider effect of RelB on the classical NFκB pathway (involving RelA and c-Rel) through cross-regulation of activation and expression in addition to RelB’s function within the alternate pathway.

Statement of novelty: We describe for the first time the immune abnormalities in patients with heterozygous RelB mutations.
Understanding the Effects of Plural Marketing Structures on Alliance Performance
Authors: Fang Eric (ER) , Lee Jongkuk , Palmatier Robert , Guo Zhaoyang
Published: January 2016

A plural alliance structure involving multiple downstream partners has become increasingly popular, yet investigations of marketing alliances continue to address mainly dyadic structures. The authors present learning and dependence balancing as key mechanisms to understand the relative performance differences between plural and dyadic structures, as well as the determinants of effective collaboration in a plural structure. Two complementary studies test the performance of plural and dyadic structures in a wide range of high-tech industries. The analysis of both plural and dyadic structure alliances in an event study shows that plural structures outperform dyadic structures for the upstream firm when marketing alliances extend to product-related tasks, the upstream firm has more alliance experience, or the industry is growing fast; however, dyadic structures perform better when the upstream market is more competitive. A second study, focusing only on plural structure alliances, shows that horizontal relationship factors (i.e., market overlap and prior relationship between downstream partners) interact with the upstream firm's greater alliance experience and reputation to lead to better returns for the upstream firm.
Ellipso-Höhentopometrie
Authors: Leonhardt Klaus , Breitmeier Ulrich
Published: January 2016

Die Ellipso-Höhentopometrie misst zusätzlich
zum topometrischen Höhenprofil die örtliche Verteilung des Materials
an der Oberfläche, so dass eine hochaufgelöste
Materialidentifizierung und Kartierung und eine Korrektur der Messfehler
möglich wird.

Alle kommerziell verfügbaren optischen Profilmessgeräte zeigen
Artefakte an Übergängen verschiedener Materialien einer
Werkstückoberfläche. Beispielsweise wird ein
Ölfilm auf einem Metalluntergrund mit nichtlinear invertierter
Profilamplitude wiedergegeben, so dass er als Vertiefung
anstatt als Erhebung gemessen wird [4,5]. Die Höhe einer Leiterbahn
auf einer mikroelektronischen Struktur wird gleichermaßen falsch
gemessen. Große,
nichtkalibrierbare Fehler können bei Schichtstrukturen
auftreten. Mit einem neuartigen
Ellipso-Höhentopometer können diese Messfehler
und Artefakte durch Korrektur des Phasensprungs bei der Lichtreflexion
für quasiplanare Oberflächen kompensiert werden.
Dazu wird aus denselben Datensätzen, die zur
Berechnung der Höhentopografie H(x, y) aufgenommen
wurden, die lokale Verteilung der Materialien und ihrer
optischen Parameter ermittelt und daraus die Material-
und Schicht-Resonanzphasen zur Korrektur
berechnet.

Die Ellipso-Höhentopometrie kann erstmals
strukturierte Profile im nm-Bereich mit hoher
räumlicher Auflösung optisch richtig messen und zusätzlich
lassen sich Informationen über Materialien,
Schichten und Verschmutzungen auf der Oberfläche
gewinnen.
A magyar iparoktatás „hadi útja”
Authors: Vörös Katalin
Published: January 2016

The questions of modernisation and social thinking altogether determined the modification of the roles of Hungarian vocational education during World War I. 
At the end of the 1890s, a modern, industrialised vocational education system was formed in Hungary. The education of qualified workmen was an integral part of the power rivalry among certain countries in this time period. The peak of this rivalry was signified by World War I, when results were also measured on the battle fields. During the war, by the sufficient appreciation of strategic goals of industrial production, vocational education had a more important role in the narrative of central power. However, because of the continuance of wars, widely new problems and tasks emerged. 
The nursing and rehabilitation of injured soldiers returning home from World War I meant new challenges towards forming the idea of a “caring” government. Vocational education and the national social policy became closely linked as a result of the armaments events after 1914, which Act XV of 1915 also proves. The concept of vocational education was redefined. New types of ’industrial vocational schools’ (schools for the war invalids) appeared. Their task was not to serve market interests primarily, but to improve the living conditions of handicapped and disabled people. Old institutions also had to adapt to the changed requirements. They not only took part in military production or handed over buildings to accommodate soldiers but also latched on to the aftercare of disabled soldiers by organizing different types of courses. 
This paper aims to show the history of the Hungarian vocational education during World War I, focusing on the relation between the vocational education and the rehabilitation of the injured soldiers as well as the schools for the warinvalids. The research was based on archival resources, school reports, applicable legislation, and the contemporary media. The study pays particular attention to the education structure and to the effect of the war on the hinterland and the national social policy. 
Fotó: Pozsonyi Kifli
Kérészy Zoltánnak, a m. kir. Erzsébet Tudományegyetem magyar alkotmány- és jogtörténet nyilvános rendes tanárának, az egyházjog és a politika jogosított tanárának életrajzi adattára és életútleírása
Authors: Kovács I. Gábor
Published: January 2016

Évek óta foglalkozunk a magyarországi polgári korszak (1848–1944) tudáselitjének, s kiváltképpen az ennek magját képező egyetemi tanárságnak a kutatásával. Az adott időszakban összesen 1044 egyetemi tanár működött Magyarország egyetemein. Rájuk vonatkozóan meglehetősen sok adatot gyűjtöttünk össze. Elsősorban arra voltunk kíváncsiak, hogy milyen szociokulturális, etnokulturális alakzatokból, felekezeti-művelődési tömbökből kerültek a tudáselit csúcsára. Ezért sok adatot gyűjtöttünk a felmenőkről, felekezeti hovatartozásukról, iskolázásukról, foglalkozásukról. Most az Északkelet-Magyarországon született református vallású egyetemi tanárokkal foglalkozunk, akiknek az ősei kivétel nélkül kisnemesek voltak, nagy arányban tanultak a Sárospataki Református Kollégiumban, s többnyire református lelkészként vagy tanárként szolgáltak. Közülük egyedül Kérészy Zoltán lett a pozsonyi, majd a pécsi tudományegyetem tanára. Egyébként ennek az egyetemnek az 1944-ig kinevezett 86 tanára közül mindössze kilencen voltak reformátusok. Érdekes az is, hogy Kérészy, akinek az ősei a 18. század elejétől rendre református lelkészek voltak, a jogtudomány terén a legmaradandóbbat azzal alkotta, hogy megírta a Katolikus Egyházi Jog a Codex Iuris Canonici alapján című művet. Az ő adattárát, életútját igyekeztünk rekonstruálni az adattárainkban korábban kialakított címszavak sorrendjében.
Egy modern intézmény hatástörténete
Authors: Csibi Norbert
Published: January 2016

Sasfi Csaba: Gimnazisták és társadalom Magyarországon a 19. század első felében. Budapest, 2013. 446 oldal. (Korall Társadalomtörténeti Monográfiák 4.)
Astrocytes as secretory cells of the central nervous system: idiosyncrasies of vesicular secretion
Authors: Verkhratsky Alexei , Matteoli Michela , Parpura Vladimir , Mothet Jean‐Pierre , Zorec Robert
Published: January 2016

Astrocytes are housekeepers of the central nervous system (CNS) and are important for CNS development, homeostasis and defence. They communicate with neurones and other glial cells through the release of signalling molecules. Astrocytes secrete a wide array of classic neurotransmitters, neuromodulators and hormones, as well as metabolic, trophic and plastic factors, all of which contribute to the gliocrine system. The release of neuroactive substances from astrocytes occurs through several distinct pathways that include diffusion through plasmalemmal channels, translocation by multiple transporters and regulated exocytosis. As in other eukaryotic cells, exocytotic secretion from astrocytes involves divergent secretory organelles (synaptic‐like microvesicles, dense‐core vesicles, lysosomes, exosomes and ectosomes), which differ in size, origin, cargo, membrane composition, dynamics and functions. In this review, we summarize the features and functions of secretory organelles in astrocytes. We focus on the biogenesis and trafficking of secretory organelles and on the regulation of the exocytotic secretory system in the context of healthy and diseased astrocytes.
Microtubule‐binding protein doublecortin‐like kinase 1 (DCLK1) guides kinesin‐3‐mediated cargo transport to dendrites
Authors: Lipka Joanna , Kapitein Lukas C , Jaworski Jacek , Hoogenraad Casper C
Published: January 2016

In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule‐binding proteins can steer polarized transport. By screening all 45 kinesin family members, we systematically addressed which kinesin motors can translocate cargo in living cells and drive polarized transport in hippocampal neurons. While the majority of kinesin motors transport cargo selectively into axons, we identified five members of the kinesin‐3 (KIF1) and kinesin‐4 (KIF21) subfamily that can also target dendrites. We found that microtubule‐binding protein doublecortin‐like kinase 1 (DCLK1) labels a subset of dendritic microtubules and is required for KIF1‐dependent dense‐core vesicles (DCVs) trafficking into dendrites and dendrite development. Our study demonstrates that microtubule‐binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport.
Somatically mutated ABL1 is an actionable and essential NSCLC survival gene
Authors: Testoni Ewelina , Stephenson Natalie L , Torres‐Ayuso Pedro , Marusiak Anna A , Trotter Eleanor W , Hudson Andrew , Hodgkinson Cassandra L , Morrow Christopher J , Dive Caroline , Brognard John
Published: January 2016

The lack of actionable mutations in patients with non‐small cell lung cancer (NSCLC) presents a significant hurdle in the design of targeted therapies for this disease. Here, we identify somatically mutated ABL1 as a genetic dependency that is required to maintain NSCLC cell survival. We demonstrate that NSCLC cells with ABL1 mutations are sensitive to ABL inhibitors and we verify that the drug‐induced effects on cell viability are specific to pharmacological inhibition of the ABL1 kinase. Furthermore, we confirm that imatinib suppresses lung tumor growth in vivo, specifically in lung cancer cells harboring a gain‐of‐function (GOF) mutation in ABL1. Consistent with structural modeling, we demonstrate that mutations in ABL1 identified in primary NSCLC tumors and a lung cancer cell line increase downstream pathway activation compared to wild‐type ABL1. Finally, we observe that the ABL1 cancer mutants display an increased cytosolic localization, which is associated with the oncogenic properties of the ABL1 kinase. In summary, our results suggest that NSCLC patients with ABL1 mutations could be stratified for treatment with imatinib in combination with other therapies.
The Bargmann type reduction for some Lax integrable two-dimensional generalization of the relativistic Toda lattice
Authors: Hentosh O.Ye.
Published: January 2016

The possibility of applying the method of reducing upon finite-dimensional invariant subspaces, generated by the eigenvalues of the associated spectral problem, to some two-dimensional generalization of the relativistic Toda lattice with the triple matrix Lax type linearization is investigated. The Hamiltonian property and Lax-Liouville integrability of the vector fields, given by this system, on the invariant subspace related with the Bargmann type reduction are found out.
On estimates for the Jacobi transform in the space $L^{p}(\mathbb{R}^{+}, J^{\alpha,\beta}(x)dx)$
Authors: El Ouadih S. , Daher R.
Published: January 2016

In this paper, we prove the estimates for the Jacobi transform in $L^{p}(\mathbb{R}^{+}, J^{\alpha,\beta}(x)dx)$ as applied to some classes of functions characterized by a generalized modulus of continuity.
Geometry of hypersurfaces of a quarter symmetric non metric connection in a quasi-Sasakian manifold
Authors: Rahman Sh.
Published: January 2016

The purpose of the paper is to study the notion of CR-submanifold and the existence of some structures on a hypersurface of a quarter symmetric non metric connection in a quasi-Sasakian manifold. We study the existence of a Kahler structure on $M$ and the existence of a globally metric frame $f$-structure in sence of Goldberg S.I., Yano K. We discuss the integrability of distributions on $M$ and geometry of their leaves. We have tries to relate this result with those before obtained by Goldberg V., Rosca R. devoted to Sasakian manifold and conformal connections.
Strictly diagonal holomorphic functions on Banach spaces
Authors: Fedak O.I. , Zagorodnyuk A.V.
Published: January 2016

In this paper we investigate the boundedness of holomorphic functionals on a Banach space with a normalized basis $\{e_n\}$ which have a very special form $f(x)=f(0)+\sum_{n=1}^\infty c_nx_n^n$ and which we call strictly diagonal. We consider under which conditions strictly diagonal functions are entire and uniformly continuous on every ball of a fixed radius.
Isolation of a Hypomorphic skn-1 Allele That Does Not Require a Balancer for Maintenance
Authors: Tang Lanlan , Dodd William , Choe Keith
Published: January 2016

In Caenorhabditis elegans, the transcription factor SKN-1 has emerged as a central coordinator of stress responses and longevity, increasing the need for genetic tools to study its regulation and function. However, current loss-of-function alleles cause fully penetrant maternal effect embryonic lethality, and must be maintained with genetic balancers that require careful monitoring and labor intensive strategies to obtain large populations. In this study, we identified a strong, but viable skn-1 hypomorphic allele skn-1(zj15) from a genetic screen for suppressors of wdr-23, a direct regulator of the transcription factor. skn-1(zj15) is a point mutation in an intron that causes mis-splicing of a fraction of mRNA, and strongly reduces wildtype mRNA levels of the two long skn-1a/c variants. The skn-1(zj15) allele reduces detoxification gene expression and stress resistance to levels comparable to skn-1 RNAi, but, unlike RNAi, it is not restricted from some tissues. We also show that skn-1(zj15) is epistatic to canonical upstream regulators, demonstrating its utility for genetic analysis of skn-1 function and regulation in cases where large numbers of worms are needed, a balancer is problematic, diet is varied, or RNAi cannot be used.
Exploring a Nonmodel Teleost Genome Through RAD Sequencing—Linkage Mapping in Common Pandora, Pagellus erythrinus and Comparative Genomic Analysis
Authors: Manousaki Tereza , Tsakogiannis Alexandros , Taggart John B , Palaiokostas Christos , Tsaparis Dimitris , Lagnel Jacques , Chatziplis Dimitrios , Magoulas Antonios , Papandroulakis Nikos , Mylonas Constantinos C , Tsigenopoulos Costas S
Published: January 2016

Common pandora (Pagellus erythrinus) is a benthopelagic marine fish belonging to the teleost family Sparidae, and a newly recruited species in Mediterranean aquaculture. The paucity of genetic information relating to sparids, despite their growing economic value for aquaculture, provides the impetus for exploring the genomics of this fish group. Genomic tool development, such as genetic linkage maps provision, lays the groundwork for linking genotype to phenotype, allowing fine-mapping of loci responsible for beneficial traits. In this study, we applied ddRAD methodology to identify polymorphic markers in a full-sib family of common pandora. Employing the Illumina MiSeq platform, we sampled and sequenced a size-selected genomic fraction of 99 individuals, which led to the identification of 920 polymorphic loci. Downstream mapping analysis resulted in the construction of 24 robust linkage groups, corresponding to the karyotype of the species. The common pandora linkage map showed varying degrees of conserved synteny with four other teleost genomes, namely the European seabass (Dicentrarchus labrax), Nile tilapia (Oreochromis niloticus), stickleback (Gasterosteus aculeatus), and medaka (Oryzias latipes), suggesting a conserved genomic evolution in Sparidae. Our work exploits the possibilities of genotyping by sequencing to gain novel insights into genome structure and evolution. Such information will boost the study of cultured species and will set the foundation for a deeper understanding of the complex evolutionary history of teleosts.
Genetic Correlates of Individual Differences in Sleep Behavior of Free-Living Great Tits (Parus major)
Authors: Stuber Erica F , Baumgartner Christine , Dingemanse Niels J , Kempenaers Bart , Mueller Jakob C
Published: January 2016

Within populations, free-living birds display considerable variation in observable sleep behaviors, reflecting dynamic interactions between individuals and their environment. Genes are expected to contribute to repeatable between-individual differences in sleep behaviors, which may be associated with individual fitness. We identified and genotyped polymorphisms in nine candidate genes for sleep, and measured five repeatable sleep behaviors in free-living great tits (Parus major), partly replicating a previous study in blue tits (Cyanistes caeruleus). Microsatellites in the CLOCK and NPAS2 clock genes exhibited an association with sleep duration relative to night length, and morning latency to exit the nest box, respectively. Furthermore, microsatellites in the NPSR1 and PCSK2 genes associated with relative sleep duration and proportion of time spent awake at night, respectively. Given the detection rate of associations in the same models run with random markers instead of candidate genes, we expected two associations to arise by chance. The detection of four associations between candidate genes and sleep, however, suggests that clock genes, a clock-related gene, or a gene involved in the melanocortin system, could play key roles in maintaining phenotypic variation in sleep behavior in avian populations. Knowledge of the genetic architecture underlying sleep behavior in the wild is important because it will enable ecologists to assess the evolution of sleep in response to selection.
The Effects of Both Recent and Long-Term Selection and Genetic Drift Are Readily Evident in North American Barley Breeding Populations
Authors: Poets Ana M , Mohammadi Mohsen , Seth Kiran , Wang Hongyun , Kono Thomas J Y , Fang Zhou , Muehlbauer Gary J , Smith Kevin P , Morrell Peter L
Published: January 2016

Barley was introduced to North America ∼400 yr ago but adaptation to modern production environments is more recent. Comparisons of allele frequencies among growth habits and spike (inflorescence) types in North America indicate that significant genetic differentiation has accumulated in a relatively short evolutionary time span. Allele frequency differentiation is greatest among barley with two-row vs. six-row spikes, followed by spring vs. winter growth habit. Large changes in allele frequency among breeding programs suggest a major contribution of genetic drift and linked selection on genetic variation. Despite this, comparisons of 3613 modern North American cultivated barley breeding lines that differ for spike-type and growth habit permit the discovery of 142 single nucleotide polymorphism (SNP) outliers putatively linked to targets of selection. For example, SNPs within the Cbf4, Ppd-H1, and Vrn-H1 loci, which have previously been associated with agronomically adaptive phenotypes, are identified as outliers. Analysis of extended haplotype sharing identifies genomic regions shared within and among breeding populations, suggestive of a number of genomic regions subject to recent selection. Finally, we are able to identify recent bouts of gene flow between breeding populations that could point to the sharing of agronomically adaptive variation. These results are supported by pedigrees and breeders’ understanding of germplasm sharing.
Whole-Genome Sequencing of a Canine Family Trio Reveals a FAM83G Variant Associated with Hereditary Footpad Hyperkeratosis
Authors: Sayyab Shumaila , Viluma Agnese , Bergvall Kerstin , Brunberg Emma , Jagannathan Vidhya , Leeb Tosso , Andersson Göran , Bergström Tomas F
Published: January 2016

Over 250 Mendelian traits and disorders, caused by rare alleles have been mapped in the canine genome. Although each disease is rare in the dog as a species, they are collectively common and have major impact on canine health. With SNP-based genotyping arrays, genome-wide association studies (GWAS) have proven to be a powerful method to map the genomic region of interest when 10–20 cases and 10–20 controls are available. However, to identify the genetic variant in associated regions, fine-mapping and targeted resequencing is required. Here we present a new approach using whole-genome sequencing (WGS) of a family trio without prior GWAS. As a proof-of-concept, we chose an autosomal recessive disease known as hereditary footpad hyperkeratosis (HFH) in Kromfohrländer dogs. To our knowledge, this is the first time this family trio WGS-approach has been used successfully to identify a genetic variant that perfectly segregates with a canine disorder. The sequencing of three Kromfohrländer dogs from a family trio (an affected offspring and both its healthy parents) resulted in an average genome coverage of 9.2X per individual. After applying stringent filtering criteria for candidate causative coding variants, 527 single nucleotide variants (SNVs) and 15 indels were found to be homozygous in the affected offspring and heterozygous in the parents. Using the computer software packages ANNOVAR and SIFT to functionally annotate coding sequence differences, and to predict their functional effect, resulted in seven candidate variants located in six different genes. Of these, only FAM83G:c155G > C (p.R52P) was found to be concordant in eight additional cases, and 16 healthy Kromfohrländer dogs.
FW: An R Package for Finlay–Wilkinson Regression that Incorporates Genomic/Pedigree Information and Covariance Structures Between Environments
Authors: Lian Lian , de los Campos Gustavo
Published: January 2016

The Finlay–Wilkinson regression (FW) is a popular method among plant breeders to describe genotype by environment interaction. The standard implementation is a two-step procedure that uses environment (sample) means as covariates in a within-line ordinary least squares (OLS) regression. This procedure can be suboptimal for at least four reasons: (1) in the first step environmental means are typically estimated without considering genetic-by-environment interactions, (2) in the second step uncertainty about the environmental means is ignored, (3) estimation is performed regarding lines and environment as fixed effects, and (4) the procedure does not incorporate genetic (either pedigree-derived or marker-derived) relationships. Su et al. proposed to address these problems using a Bayesian method that allows simultaneous estimation of environmental and genotype parameters, and allows incorporation of pedigree information. In this article we: (1) extend the model presented by Su et al. to allow integration of genomic information [e.g., single nucleotide polymorphism (SNP)] and covariance between environments, (2) present an R package (FW) that implements these methods, and (3) illustrate the use of the package using examples based on real data. The FW R package implements both the two-step OLS method and a full Bayesian approach for Finlay–Wilkinson regression with a very simple interface. Using a real wheat data set we demonstrate that the prediction accuracy of the Bayesian approach is consistently higher than the one achieved by the two-step OLS method.
Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans
Authors: Brenner John L , Schedl Tim
Published: January 2016

Germline stem cell differentiation in Caenorhabditis elegans is controlled by glp-1 Notch signaling. Cell fate regulator GLD-1 is sufficient to induce meiotic entry and expressed at a high level during meiotic prophase, inhibiting mitotic gene activity. glp-1 signaling and other regulators control GLD-1 levels post-transcriptionally (low in stem cells, high in meiotic prophase), but many aspects of GLD-1 regulation are uncharacterized, including the link between glp-1-mediated transcriptional control and post-transcriptional GLD-1 regulation. We established a sensitive assay to quantify GLD-1 levels across an ∼35-cell diameter field, where distal germline stem cells differentiate proximally into meiotic prophase cells in the adult C. elegans hermaphrodite, and applied the approach to mutants in known or proposed GLD-1 regulators. In wild-type GLD-1 levels elevated ∼20-fold in a sigmoidal pattern. We found that two direct transcriptional targets of glp-1 signaling, lst-1 and sygl-1, were individually required for repression of GLD-1. We determined that lst-1 and sygl-1 act in the same genetic pathway as known GLD-1 translational repressor fbf-1, while lst-1 also acts in parallel to fbf-1, linking glp-1-mediated transcriptional control and post-transcriptional GLD-1 repression. Additionally, we estimated the position in wild-type gonads where germ cells irreversibly commit to meiotic development based on GLD-1 levels in worms where glp-1 activity was manipulated to cause an irreversible fate switch. Analysis of known repressors and activators, as well as modeling the sigmoidal accumulation pattern, indicated that regulation of GLD-1 levels is largely regional, which we integrated with the current view of germline stem cell differentiation.
Phosphorus removal in a vertical flow constructed wetland using dolomite powder and chippings as filter media
Authors: Žibienė Gražina , Dapkienė Midona , Kazakevičienė Jurgita , Radzevičius Algirdas
Published: January 2016

Different kinds of natural and artificial filter media are able to retain phosphorus in the constructed wetlands. Due to the fact that the constructed wetland needs huge amounts of the filter media, it is very important to find locally available material which distinguishes itself by its ability to retain phosphorus. The materials found in Lithuania were considered and dolomite was chosen. Two dolomite fractions, dolomite powder (1–2 mm) and dolomite chippings (2–5 mm), and sand media were used in the laboratory- scale installed for the comparative experiments. The laboratory-scale with dolomite as the filter media was on average by 21% more efficient in total phosphorus removal in comparison with the sand media. Based on the laboratory research pilot–scale vertical flow constructed wetland of 160 m2 was installed and planted with reed Phragmites australis. The dolomite chippings as filter media were chosen in order to avoid the danger of the clogging of constructed wetland. Efficiency of total phosphorus removal in the pilot-scale vertical flow constructed wetland was on average 95.7%, phosphates removal – 94.8% within one year.
DE L’ENFANCE ET DES ENFANTS : ONTOLOGIE HISTORIQUE ET ANTHROPOLOGIE ARCHEOLOGIQUE DE L’ENFANCE
Authors: Mokaddem Salim
Published: January 2016

Le corps est souvent vécu dans une confrontation entre un intérieur et un extérieur (dualisme du corps et de l’âme, du sensible et de l’intellect, de la forme esthétique et de la densité noétique) qui se fait au détriment d’une perception hétérogène ou diversifiée du corps puisque l’âme devient alors la prison du corps (Foucault). Trop souvent, en effet, le corps de l’enfant est rabattu sur une vision biopolitique de l’enfance comme état, âge, ou moment transitoire mineur dont il faut évacuer l’inachèvement par une biopolitique de maturité, de disciplinarisation continue, d’éducation normée, pour en évacuer la part maudite non réductible aux impératifs de contrôle par le monde adulte. Ainsi, l’enfant est écrasé par une vision érotique, médicale, esthétique, donc fortement historicisée de son être-au-monde. La fête des morts au Japon est devenu la fête des enfants ; elle révèle les ambigüités de cet état d’enfant : le corps de l’enfant est assigné à résidence et la liberté désirée de l’enfance est cependant étalée sur toutes les surfaces sociales de la société civile et religieuse (culte du « kawai », de la miniaturisation). Une philosophie des âges de la vie doit nécessairement prendre en compte l’anthropologie historique du corps et de ses limites pour comprendre ses modalités d’expression possible.
CORPO, ARTE E CONHECIMENTO: UMA TRAMA DE SENTIDOS NA FILOSOFIA DE MERLEAU-PONTY
Authors: Da Silva Adriana Maria , De Barrenechea Miguel Angel
Published: January 2016

O objetivo central desse trabalho é analisar as reflexões elaboradas por Merleau-Ponty sobre a restituição do aspecto sensível na construção do conhecimento, em que o filósofo, em nossa ótica, atribui ao corpo um estatuto educativo. Por esse caminho, a experiência artística expressa a possibilidade de restabelecer o vínculo incindível entre corpo e pensamento. A obra de arte é emblemática dos aspectos imagético, inventivo e poético da condição corpórea e, nas análises de Merleau-Ponty, ela possibilita ao corpo o resgate da sua densidade e da sua ressonância nos modos de compreender o mundo, diferindo-se do pensamento racionalista. Por conseguinte, buscamos na atividade artística outro meio de pensar a educação, destacando que essa significação recusa os pragmatismos e utilitarismos, expressos comumente na realidade educacional da atualidade, uma vez que esta se apoia apenas no entendimento e não na experiência do conhecimento. Finalmente, a educação, inspirada na filosofia de Merleau-Ponty, deve ser compreendida enquanto uma trama de sentidos, em que não há separação entre corpo e pensamento, portanto, enquanto uma experiência artística.
AVALIAÇÃO DA APRENDIZAGEM ESCOLAR: COMO OS PROFESSORES ESTÃO PRATICANDO A AVALIAÇÃO NA ESCOLA
Authors: Duarte Carlos Eduardo
Published: January 2016

A avaliação da aprendizagem escolar, obedecendo a um modelo de sociedade autoritário, tem sido utilizada, equivocadamente, como um instrumento disciplinador e classificatório, fazendo com que a reprovação deixe de ser um caso excepcional para ser uma rotina na escola. Através de uma pesquisa junto aos professores dos anos finais do ensino fundamental, constatou-se que a prática da avaliação nas escolas está muito distante da ação avaliativa diagnóstica e formativa. É preciso – e urgente – uma mudança na postura dos professores no que diz respeito à prática avaliativa para que a escola possa ser, de fato, formadora de cidadãos críticos e conscientes de seu papel na sociedade.
PRINCIPAIS ABORDAGENS TEÓRICO-METODOLÓGICAS SOBRE A TEORIA DO COMPORTAMENTO: DO POSITIVISMO AO PÓS-MODERNISMO
Authors: Ciribeli Joao Paulo , Mendes Wesley de Almeida
Published: January 2016

O artigo buscou identificar as principais correntes teórico-metodológicas que contribuíram para o avanço e o entendimento do comportamento do consumidor, bem como aferir suas nuanças, distinções e semelhanças. Quanto à metodologia, o estudo consiste num ensaio teórico, o que nos remete à revisão bibliográfica, onde pode-se confrontar as idéias dos principais autores que discorrem na área, assim como analisar, de forma didática suas arguições. Sobre os resultados, verificou-se que três modelos contribuíram de forma mais substancial ao entendimento do comportamento do consumidor: modernismo, estruturalismo e pós-modernismo. Pode-se constatar também que estes modelos podem ser agrupadas em duas abordagens, a quantitativa e a qualitativa, o que ocasionou, a posteriori, a híbrida. O modernismo, em sua abordagem quantitativa, buscava compreender o comportamento do consumidor de forma objetiva, enquanto o estruturalismo buscava conhecer as estruturas dos fatos e objetos observados. Já o pós-modernismo apresenta uma abordagem qualitativa, onde as experiências, as crenças, os valores e a carga simbólica do comportamento do indivíduo passam a preponderar sobre a análise das massas.
AULAS PRÁTICAS COMO ESTRATÉGIA PARA O CONHECIMENTO EM BOTÂNICA NO ENSINO FUNDAMENTAL
Authors: Da Silva Ana Paula Miranda , Silva Maria Francilene Souza , Rodrigues da Rocha Francinalda Maria , De Andrade Ivanilza Moreira
Published: January 2016

Na aplicação dos conteúdos da disciplina Ciências Naturais a teoria não deveria ser desvinculada da prática, pois esta metodologia é imprescindível para construção do conhecimento científico dos alunos. Assim, objetivou-se investigar a viabilidade de aulas práticas para alunos do 7º ano do Ensino Fundamental, em duas escolas estaduais no município de Parnaíba, Piauí, como estratégias para facilitar o ensino-aprendizagem em conteúdos de Botânica. Foram aplicados questionários antes e após cada aula prática para verificar a construção de conhecimento. Os resultados demonstraram aumento no número de respostas corretas após as aulas práticas nas duas escolas alvo da pesquisa. Concluiu-se que o ensino de ciências, pode ser melhorado a partir de iniciativas simples, como realização de aula prática dentro da sala ou usando os espaços verdes da escola.
USO DAS IDEIAS INICIAIS DOS ESTUDANTES: UMA EXPERIÊNCIA NA FORMAÇÃO INICIAL DE PROFESSORES DE FÍSICA
Authors: Hygino Cassiana Barreto , Rosa Guilherme , Callegario Laís , Costa Leandro , Alves Vanessa , Linhares Marília Paixão
Published: January 2016

Este trabalho faz parte de uma proposta desenvolvida na formação inicial de professores de física com foco na evolução do conhecimento dos discentes sobre a atividade docente. Apresentamos a análise de uma atividade proposta na disciplina Estratégias para o Ensino de Física II, de um curso de Licenciatura em Física. A disciplina adota como caminho metodológico o método de estudos de caso, focalizando questões relevantes da prática docente. Analisamos, no presente artigo, o estudo de caso intitulado: “Ideias prévias no processo de aprendizagem de Física”, o qual teve como objetivo identificar as concepções dos futuros docentes sobre as formas de acessar e considerar as ideias prévias dos alunos. Os dados foram coletados a partir das respostas de cinco licenciandos nos passos inicial e final do estudo de caso. As análises indicaram que ao fim do processo os futuros docentes apresentaram concepções mais próximas de modelos desejáveis.
AVALIAÇÃO DE SISTEMA DE COLUNAS PARA REMEDIAÇÃO DE BIOGÁS A PARTIR DE BIOMASSA NÃO DIGERIDA
Authors: Pereira Vinicius , Ferreira Junior Jose Mario , Martinez Gustavo Aristides Santana , Tomachuk Celia Regina
Published: January 2016

O biogás é produto de reações de biomassa e constitui um combustível gasoso em temperatura ambiente, com conteúdo energético elevado comparável aos combustíveis de origem fósseis e semelhante ao gás natural. Devido a seu poder energético pode ser utilizado para geração de energia elétrica, térmica ou mecânica, levando a redução dos custos de produção. O processo de biodigestão anaeróbia consiste em metabolizar substâncias orgânicas complexas produzindo metano, dióxido de carbono, gases em menor quantidade (sulfídrico e hidrogênio) e material celular. Como subproduto da produção de metano, o dióxido de carbono é o que apresenta maior quantidade, e por ser um gás não-inflamável, pode ser definido como um subproduto indesejável da produção de biogás. Este trabalho teve como objetivo separar o dióxido de carbono, produzido na fermentação anaeróbica, por precipitação em reação com hidróxido de bário; o produto gerado é o carbonato de bário, o qual é tóxico e utilizado por diferentes indústrias. O processo adotado objetiva encontrar uma metodologia e aproveitar economicamente o dióxido de carbono gerado em biodigestores diminuindo o efeito estufa provocado pela emissão do mesmo no meio ambiente.
EPIDEMIOLOGY AND PLACE: A SPATIAL AREA FOR HEALTH ANALYSIS
Authors: Leite Maria Jalila Vieira de Figueirêdo , Dantas Aldo , Roncalli Angelo Giuseppe
Published: January 2016

The authors present a theoretical discussion related to spatial areas used in Epidemiology, analysing their theoretical and methodological strengths and weaknesses in the recognition of social determinants of health. Some territorial divisions were defended as alternative scales because of homogeneity and accuracy characteristics, and because of their particular criteria of definitions, while recognizing the necessity to overcome some challenges related to the concepts, research designs and access to data available in these scales.
O PANORAMA DAS PUBLICAÇÕES SOBRE EDUCAÇÃO AMBIENTAL NA EDUCAÇÃO DE JOVENS E ADULTOS NOS ÚLTIMOS DEZ ANOS (2005-2014)
Authors: Alkimin Gilberto Dias de
Published: January 2016

A presente pesquisa teve como objetivo analisar a inserção da Educação Ambiental (EA) na Educação de Jovens e Adultos (EJA) brasileira nos últimos dez anos (2005-2014) e realizar um panorama da mesma. Para tanto, a pesquisa do tipo estado da arte foi realizada selecionando-se oito revistas de acordo com sua classificação Qualis de periódicos da CAPES, os artigos selecionados, de acordo com o tema de interesse foram analisados e então procedeu-se a construção de um panorama da inserção da EA na EJA. Do total de trabalhos encontrados nesse período apenas 1,42% falam sobre o assunto em questão, desses três envolvem a educação não-formal e seis a educação formal. O tipo de pesquisa predominante é a participativa e as atividades envolvem aulas teórico-práticas, dinâmicas e jogos. A percepção de meio ambiente dos envolvidos é naturalista. Pode-se dizer que a inserção da EA na EJA no Brasil é algo insipiente, porém necessária. É preciso uma pesquisa mais abrangente sobre o estado da arte a real inserção da EA na EJA no país.
CAIXAS PRETAS
Authors: De Paula Enio Freire
Published: January 2016

Resenha da Obra:TOROK, Simon, HOLPER, Paul. Inventando Milhões. Tradução: Carolina Paganine. Campinas: Editora da UNICAMP, 2009, 256 páginas.
MODELO MATEMÁTICO PARA CORTES DE BARRAS DE AÇO NO PROCESSO DE FABRICAÇÃO DE TRIÂNGULOS
Authors: Wavrzynczak Hione Cleder , Ulbricht Gerson , Teixeira Edson Sidnei Maciel
Published: January 2016

Uma das preocupações das empresas em seus processos fabris é quanto a melhor utilização da matéria-prima, trazendo como consequência a redução ou elevação no custo de fabricação. Sendo assim, no desenvolvimento deste trabalho é apresentado um modelo matemático de otimização com objetivo de minimizar o desperdício de matéria-prima com o uso de ferramentas da pesquisa operacional. Trata-se assim, de um problema de corte unidimensional de barras de aço cilíndricas para a fabricação de triângulos. Em um primeiro estágio foi estabelecido o maior número possível de padrões de corte e em um segundo estágio, foram definidos quais padrões seriam utilizados em quantidade para atender às demandas previstas. Para validação do modelo, este foi aplicado em uma empresa fabricante de instrumentos musicais, no processo de fabricação de triângulos de quatro medidas onde foi trabalhado com corte de barras, buscando reduzir ao máximo os desperdícios. Assim, obteve-se uma perda teórica de 0,3% do total de barras de aço, menos do que a empresa atinge atualmente. Os testes realizados tem mostrado boas perspectivas quanto à redução significativa nos desperdícios o que demonstra que o modelo se apresenta como um boa ferramenta no auxílio à decisão da gestão de recursos.
LAS TECNOLOGÍAS DE LA INFORMACIÓN Y LAS COMUNICACIONES COMO HERRAMIENTAS PARA LA GESTIÓN DEL PATRIMONIO CULTURAL CON UNA VISIÓN EMPRENDEDORA
Authors: Valdespino Tamayo Cecilia , Leite Emanuel
Published: January 2016

El patrimonio cultural no se limita a monumentos y colecciones de objetos, comprende también tradiciones o expresiones vivas heredadas de nuestros antepasados y transmitidas a nuestros descendientes. La importancia de este recurso inmaterial no radica solo en la manifestación cultural en sí, sino en el cúmulo de conocimientos y técnicas que se transmiten de generación en generación. Se basa en el reconocimiento por las comunidades, grupos o individuos que lo crean, mantienen y transmiten. La difusión es uno de los tres pilares de la gestión del patrimonio. Su definición merece una revisión a la luz de nuevas disciplinas y estrategias de comunicación incorporadas en los últimos diez años. El espíritu empresarial y la visión emprendedora junto al desarrollo tecnológico están generando un profundo impacto en todos los ámbitos de la vida diaria, desencadenando aspectos positivos en la gestión y conservación del patrimonio cultural. En este trabajo se da a conocer el papel que han desempeñado las Tecnologías de la Información y las Comunicaciones, como herramienta insustituible, en la difusión del patrimonio. Se describe el aporte de  tres de las principales líneas de desarrollo de software a este pilar fundamental (Realidad Virtual, Sistemas de Información Geográfica y Portales WEB), las herramientas y tecnologías más utilizadas en estas ramas, así como los sistemas informáticos desarrollados a partir de investigaciones científicas realizadas  en la Carrera de Ingeniería en Ciencias Informáticas de la Universidad de Granma, como resultado de un trabajo intencionado realizado con la dirección de Patrimonio y  Cultura en la provincia.