bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensor Fusion-Based Anthropomorphic Control of a Robotic Arm
Authors: Chen Furong , Wang Feilong , Dong Yanling , Yong Qi , Yang Xiaolong , Zheng Long , Gao Yi , Su Hang
Published: October 2023

The main goal of this research is to develop a highly advanced anthropomorphic control system utilizing multiple sensor technologies to achieve precise control of a robotic arm. Combining Kinect and IMU sensors, together with a data glove, we aim to create a multimodal sensor system for capturing rich information of human upper body movements. Specifically, the four angles of upper limb joints are collected using the Kinect sensor and IMU sensor. In order to improve the accuracy and stability of motion tracking, we use the Kalman filter method to fuse the Kinect and IMU data. In addition, we introduce data glove technology to collect the angle information of the wrist and fingers in seven different directions. The integration and fusion of multiple sensors provides us with full control over the robotic arm, giving it flexibility with 11 degrees of freedom. We successfully achieved a variety of anthropomorphic movements, including shoulder flexion, abduction, rotation, elbow flexion, and fine movements of the wrist and fingers. Most importantly, our experimental results demonstrate that the anthropomorphic control system we developed is highly accurate, real-time, and operable. In summary, the contribution of this study lies in the creation of a multimodal sensor system capable of capturing and precisely controlling human upper limb movements, which provides a solid foundation for the future development of anthropomorphic control technologies. This technology has a wide range of application prospects and can be used for rehabilitation in the medical field, robot collaboration in industrial automation, and immersive experience in virtual reality environments.
Tumor-Associated and Systemic Autoimmunity in Pre-Clinical Breast Cancer among Post-Menopausal Women
Authors: Parks Christine G. , Wilson Lauren E. , Capello Michela , Deane Kevin D. , Hanash Samir M.
Published: October 2023

Autoantibodies to tumor-associated antigens (anti-TAA) are potential biomarkers for breast cancer, but their relationship systemic autoimmunity as ascertained though antinuclear antibodies (ANA) is unknown and warrants consideration given the common occurrence of autoimmunity and autoimmune diseases among women. The relationship between anti-TAAs and ANA among women who were later diagnosed with breast cancer and others who remained cancer free in the Women’s Health Initiative cohort. The study sample included 145 post-menopausal women with baseline ANA data. A total of 37 ANA-positive women who developed breast cancer (i.e., cases; mean time to diagnosis 6.8 years [SE 3.9]) were matched to a random sample of 36 ANA-negative cases by age and time to diagnosis. An age-matched control sample was selected including 35 ANA-positive and 37 ANA-negative women who did not develop breast cancer (i.e., controls; follow-up time ~13 years [SE 3]). Baseline sera were assessed for Immunoglobulin G (IgG) antibodies, measured by custom microarray for 171 breast and other cancer-associated TAA. We used linear regression to estimate cross-sectional associations of ANA with log-transformed anti-TAA among cases and controls. Most anti-TAA did not vary by ANA status. Two anti-TAA were elevated in ANA-positive compared to ANA-negative cases: anti-PGM3 (p = 0.004) and anti-TTN (p = 0.005, especially in cases up to 7 years before diagnosis, p = 0.002). Anti-TAA antibodies were not generally related to ANA, a common marker of systemic autoimmunity. Associations of ANA with particular antigens inducing autoimmunity prior to breast cancer warrant further investigation.
NBL1 Reduces Corneal Fibrosis and Scar Formation after Wounding
Authors: Tsai Chi-Hao , Liu Emily , Phan Andrew , Lu Krystal Lynn , Mei Hua
Published: October 2023

Corneal scarring is a leading cause of blindness. Currently, there is no treatment to prevent and/or reduce corneal scar formation under pathological conditions. Our previous data showed that the NBL1 protein, also termed the DAN Family BMP (Bone morphogenetic protein) Antagonist, was highly expressed in corneal stromal cells upon wounding. Here, we examined the function of NBL1 in corneal wound healing. Mouse corneas were mechanically wounded, followed by a 2-week treatment using NBL1. Wounded corneas treated with vehicle or an Fc tag served as controls. Compared with the controls, NBL1 treatment facilitated wound re-epithelialization, partially restored the stromal thickness, and significantly reduced corneal scar formation. NBL1 treatment did not decrease immune cell infiltration, indicating that the anti-scarring effect was not dependent on immune suppression. We further examined the anti-fibrotic effect of NBL1 on human corneas. Pairs of human corneas were induced to form myofibroblasts (a key player in fibrosis and scarring) upon wounding and incubation in a medium containing TGF-β1. The OS corneas were treated with Fc as a control, and the OD corneas were treated with NBL1. Compared with the control, human corneas treated with NBL1 had significantly fewer myofibroblasts, which was consistent with these mouse data. A further study revealed that NBL1 treatment inhibited BMP canonical (phospho-Smad1/5) and no-canonical (phospho-p38) pathways in human corneas. Data show that NBL1 reduced corneal fibrosis and scar formation in mice and cultured human corneas. The underlying molecular mechanism is not certain because both anti-fibrotic Smad1/5 and pro-fibrotic p38 pathways were inhibited upon NBL1 treatment. Whether the p38 pathway dominates the Smad1/5 pathway during corneal fibrosis, leading to the anti-fibrotic effect of NBL1, needs further investigation.
Transferrin-Modified Triptolide Liposome Targeting Enhances Anti-Hepatocellular Carcinoma Effects
Authors: Zhao Xiaoli , Yang Yifan , Su Xuerong , Xie Ying , Liang Yiyao , Zhou Tong , Wu Yangqian , Di Liuqing
Published: October 2023

Triptolide (TP) is an epoxy diterpene lactone compound isolated and purified from the traditional Chinese medicinal plant Tripterygium wilfordii Hook. f., which has been shown to inhibit the proliferation of hepatocellular carcinoma. However, due to problems with solubility, bioavailability, and adverse effects, the use and effectiveness of the drug are limited. In this study, a transferrin-modified TP liposome (TF-TP@LIP) was constructed for the delivery of TP. The thin-film hydration method was used to prepare TF-TP@LIP. The physicochemical properties, drug loading, particle size, polydispersity coefficient, and zeta potential of the liposomes were examined. The inhibitory effects of TF-TP@LIP on tumor cells in vitro were assessed using the HepG2 cell line. The biodistribution of TF-TP@LIP and its anti-tumor effects were investigated in tumor-bearing nude mice. The results showed that TF-TP@LIP was spherical, had a particle size of 130.33 ± 1.89 nm and zeta potential of −23.20 ± 0.90 mV, and was electronegative. Encapsulation and drug loading were 85.33 ± 0.41% and 9.96 ± 0.21%, respectively. The preparation was stable in serum over 24 h and showed biocompatibility and slow release of the drug. Flow cytometry and fluorescence microscopy showed that uptake of TF-TP@LIP was significantly higher than that of TP@LIP (p < 0.05), while MTT assays indicated mean median inhibition concentrations (IC50) of TP, TP@LIP, and TF-TP@ of 90.6 nM, 56.1 nM, and 42.3 nM, respectively, in HepG2 cell treated for 48 h. Real-time fluorescence imaging indicated a significant accumulation of DiR-labeled TF-TP@LIPs at tumor sites in nude mice, in contrast to DiR-only or DiR-labeled, indicating that modification with transferrin enhanced drug targeting to the tumor tissues. Compared with the TP and TP@LIP groups, the TF-TP@LIP group had a significant inhibitory effect on tumor growth. H&E staining results showed that TF-TP@LIP inhibited tumor growth and did not induce any significant pathological changes in the heart, liver, spleen, and kidneys of nude mice, with all liver and kidney indices within the normal range, with no significant differences compared with the control group, indicating the safety of the preparation. The findings indicated that modification by transferrin significantly enhanced the tumor-targeting ability of the liposomes and improved their anti-tumor effects in vivo. Reducing its distribution in normal tissues and decreasing its toxic effects suggest that the potential of TF-TP@LIP warrants further investigation for its clinical application.
Expression of CD40 and CD192 in Classical Monocytes in Multiple Sclerosis Patients Assessed with Transcranial Magnetic Stimulation
Authors: Režić Mužinić Nikolina , Markotić Anita , Pavelin Sanda , Polančec Denis , Buljubašić Šoda Maja , Bralić Antonia , Šoda Joško , Mastelić Angela , Mikac Una , Jerković Ana , Rogić Vidaković Maja
Published: October 2023

Expression of CD40 and CD192 markers in different monocyte subpopulations has been reported to be altered in people with MS (pwMS). Also, functional connectivity of the corticospinal motor system pathway alterations has been proved by transcranial magnetic stimulation (TMS). The study objective was to investigate the expression of CD40 and CD192 in classical (CD14++CD16−), intermediate CD14++CD16+ and non-classical (CD14+CD16++) blood monocyte subpopulations in pwMS, undergoing neurophysiological TMS assessment of the corticospinal tract integrity by recording motor-evoked potentials (MEPs). Radiological examination on lesion detection with MRI was performed for 23 patients with relapsing–remitting MS treated with teriflunomide. Then, immunological analysis was conducted on peripheral blood samples collected from the patients and 10 healthy controls (HC). The blood samples were incubated with anti-human CD14, CD16, CD40 and CD192 antibodies. Next, pwMS underwent neurological testing of functional disability (EDSS) and TMS assessment with recording MEPs from upper and lower extremity muscles. The results show that in comparison to HC subjects, both pwMS with normal and altered MEP findings (prolonged MEP latency or absent MEP response) had significantly decreased surface receptor expression measured (MFIs) of CD192 and increased CD40 MFI in classical monocytes, and significantly increased percentages of classical and total monocytes positive for CD40. Knowing CD40’s pro-inflammatory action, and CD192 as a molecule that enables the passing of monocytes into the brain, decreased CD192 in classical monocytes could represent a beneficial anti-inflammatory parameter.
Surface Functionalised Optical Fibre for Detection of Hydrogen Sulphide
Authors: Baghapour Shaghayegh , Nehema Jasmine , Zhang Wen Qi , Warren-Smith Stephen C. , Hickey Shane M. , Plush Sally E. , Afshar Vahid Shahraam
Published: October 2023

Dysregulated production of hydrogen sulphide in the human body has been associated with various diseases including cancer, underlining the importance of accurate detection of this molecule. Here, we report the detection of hydrogen sulphide using fluorescence-emission enhancement of two 1,8-naphthalimide fluorescent probes with an azide moiety in position 4. One probe, serving as a control, featured a methoxyethyl moiety through the imide to evaluate its effectiveness for hydrogen sulphide detection, while the other probe was modified with (3-aminopropyl)triethoxysilane (APTES) to enable direct covalent attachment to an optical fibre tip. We coated the optical fibre tip relatively homogeneously with the APTES-azide fluorophore, as confirmed via x-ray photoelectron spectroscopy (XPS). The absorption and fluorescence responses of the control fluorophore free in PBS were analysed using UV-Vis and fluorescence spectrophotometry, while the fluorescence emission of the APTES-azide fluorophore-coated optical fibres was examined using a simple, low-cost optical fibre-based setup. Both fluorescent probes exhibited a significant increase (more than double the initial value) in fluorescence emission upon the addition of HS− when excited with 405 nm. However, the fluorescence enhancement of the coated optical fibres demonstrated a much faster response time of 2 min (time for the fluorescence intensity to reach 90% of its maximum value) compared to the control fluorophore in solution (30 min). Additionally, the temporal evolution of fluorescence intensity of the fluorophore coated on the optical fibre was studied at two pH values (7.4 and 6.4), demonstrating a reasonable overlap and confirming the compound pH insensitivity within this range. The promising results from this study indicate the potential for developing an optical fibre-based sensing system for HS− detection using the synthesised fluorophore, which could have significant applications in health monitoring and disease detection.
Topoisomerase 1 Activity Is Reduced in Response to Thermal Stress in Fruit Flies and in Human HeLa Cells
Authors: Juul-Kristensen Trine , Keller Josephine Geertsen , Borg Kathrine Nygaard , Hansen Noriko Y. , Foldager Amalie , Ladegaard Rasmus , Ho Yi-Ping , Loeschcke Volker , Knudsen Birgitta R.
Published: October 2023

In the modern world with climate changes and increasing pollution, different types of stress are becoming an increasing challenge. Hence, the identification of reliable biomarkers of stress and accessible sensors to measure such biomarkers are attracting increasing attention. In the current study, we demonstrate that the activity, but not the expression, of the ubiquitous enzyme topoisomerase 1 (TOP1), as measured in crude cell extracts by the REEAD sensor system, is markedly reduced in response to thermal stress in both fruit flies (Drosophila melanogaster) and cultivated human cells. This effect was observed in response to both mild-to-moderate long-term heat stress and more severe short-term heat stress in D. melanogaster. In cultivated HeLa cells a reduced TOP1 activity was observed in response to both cold and heat stress. The reduced TOP1 activity appeared dependent on one or more cellular pathways since the activity of purified TOP1 was unaffected by the utilized stress temperatures. We demonstrate successful quantitative measurement of TOP1 activity using an easily accessible chemiluminescence readout for REEAD pointing towards a sensor system suitable for point-of-care assessment of stress responses based on TOP1 as a biomarker.
Cross-Modal Plasticity during Self-Motion Perception
Authors: Lin Rushi , Zeng Fu , Wang Qingjun , Chen Aihua
Published: October 2023

To maintain stable and coherent perception in an ever-changing environment, the brain needs to continuously and dynamically calibrate information from multiple sensory sources, using sensory and non-sensory information in a flexible manner. Here, we review how the vestibular and visual signals are recalibrated during self-motion perception. We illustrate two different types of recalibration: one long-term cross-modal (visual–vestibular) recalibration concerning how multisensory cues recalibrate over time in response to a constant cue discrepancy, and one rapid-term cross-modal (visual–vestibular) recalibration concerning how recent prior stimuli and choices differentially affect subsequent self-motion decisions. In addition, we highlight the neural substrates of long-term visual–vestibular recalibration, with profound differences observed in neuronal recalibration across multisensory cortical areas. We suggest that multisensory recalibration is a complex process in the brain, is modulated by many factors, and requires the coordination of many distinct cortical areas. We hope this review will shed some light on research into the neural circuits of visual–vestibular recalibration and help develop a more generalized theory for cross-modal plasticity.
Latent Class Analysis on Types of Challenging Behavior in Persons with Developmental Disabilities: Focusing on Factors Affecting the Types of Challenging Behavior
Authors: Kim Daeyong
Published: October 2023

This study sought to analyze the latent classes of challenging behaviors among persons with developmental disabilities and examine the effects of related variables. To this end, the Korea Employment Agency for Persons with Disabilities collected data from the Survey on the Work and Life of Persons with Developmental Disabilities from 3000 households that included at least one family member with a developmental disability aged ≥15 years, surveying the persons themselves as well as their caregivers. As a result of the analysis, four latent classes were derived based on the types of challenging behavior and named as follows: overall challenging behavior, aggressive behavior, socially inappropriate behavior, and no challenging behavior. The main disability, disability grade, presence of multiple disabilities, disability status, activities of daily living, reading skills, writing skills, and situational awareness were significant factors affecting each latent class in the type of challenging behavior. Significant factors differed among the groups. This study identified the types of challenging behaviors and their influencing factors in a large sample of individuals with developmental disabilities and analyzed the correlation between their challenging behaviors and activities of daily living.
Correlation Analysis of Retail Space and Shopping Behavior in a Commercial Street Based on Space Syntax: A Case of Shijiazhuang, China
Authors: Lian Haitao , Li Gaomei
Published: October 2023

The visibility and spatial characteristics of commercial space are the key factors that affect the space vitality. However, the coupling visibility graphical analysis of commercial spaces and spatial characteristics to quantitatively analyse consumer behaviour in commercial street spaces and assess the vitality of retail spaces has not been adequately studied. In this paper, the aim is to conduct a visibility graph analysis of Zhuangli Street in Letai Center, Shijiazhuang, using space syntax theory, assessing the spatial vitality of the retail space by investigating the shop visits. First, a methodology for obtaining data on spatial characteristics and consumer behaviour of shopping streets was developed. Secondly, this article constructs a process for a visibility graph analysis of Zhuangli Street based on space syntax theory. Third, two combination variables of the space coefficient and depth coefficient of shop windows in retail spaces of a commercial street are proposed. Finally, the effect of combination variables and business types on spatial vitality was analyzed using correlation and multiple regression methods, and a space vitality prediction model was proposed. The results showed that the shop with the highest shop visits of retail spaces in the shopping street is 13.55 times higher than the smallest shop. The space coefficient of the shop window, depth coefficient of the shop window, and space connectivity of retail spaces in commercial streets have positive effects on space vitality. The workflow proposed in this paper can provide technical support for retail space design in commercial streets as well as evaluating and optimizing commercial street space design solutions.
Urban Building Energy Modeling with Parameterized Geometry and Detailed Thermal Zones for Complex Building Types
Authors: Xi Hongyan , Zhang Qilin , Ren Zhiyi , Li Guangchen , Chen Yixing
Published: October 2023

Urban building energy modeling (UBEM) has attracted wide attention to the requirement for global carbon emission reduction. This paper presents a UBEM tool, AutoBPS-Param, to generate building energy models (BEMs) with parameterized geometry and detailed thermal zones, especially for complex building types, considering the shading effect from surrounding buildings simultaneously. Three building number scales and four scenarios were analyzed in the hotel-related buildings in Changsha, China. For the prototype modeling of Scenario 1, eighteen prototype building energy models for six building types in three vintages were created, and their simulation results were aggregated based on their representative floor areas. For AutoBPS-Param of Scenario 4, the method created one EnergyPlus (Version: 9.3.0) model for each building. The geometry of the prototype model was scaled and modified based on the target building’s length, width, and number of stories. The surrounding buildings were also added to the AutoBPS-Param simulation to better capture the urban dynamic impact. The results showed that the annual electricity and natural gas energy use intensity (EUI) of the pre-2005 HotelOffice prototype model was 172.25 and 140.45 kWh/m2. In contrast, with the AutoBPS-Param method, the annual electricity EUIs of 71 HotelOffice buildings constructed before 2005 ranged from 159.51 to 213.58 kWh/m2 with an average of 173.14 kWh/m2, and the annual gas EUIs ranged from 68.02 to 229.12 kWh/m2 with an average of 108.89 kWh/m2. The proposed method can better capture the diversity of urban building energy consumption.
Ground Deformation Monitoring for Subway Structure Safety Based on GNSS
Authors: Tan Dongmei , Li An , Ji Baifeng , Duan Jiayi , Tao Yu , Luo Hao
Published: October 2023

Ground deformation poses a serious threat to the safety of subway structures. Consequently, intelligent and efficient automated safety monitoring of ground deformation along the subway has become urgent. Traditional engineering observation methods have the disadvantages of difficulties with datum selection, non-automation, and poor reliability. A ground deformation monitoring system for subway structure safety based on the Global Navigation Satellite System (GNSS) was established and validated through experimental comparisons with traditional precision leveling in this study. Based on the GNSS monitoring points, the continuous kinematic observation GNSS data of ground deformation along the subway line were obtained; a joint robust local mean decomposition (RLMD)–singular value decomposition (SVD) noise-reduction processing method for GNSS signals was proposed to realize the real-time and high-precision monitoring of ground deformation. The results show that the proposed combined noise-reduction method can reduce the maximum noise amplitude by 86%. When compared with the accuracy of the traditional precision leveling method, it was determined that the vertical positioning accuracy of the deformation monitoring system is greater than 2.7 mm, the horizontal positioning accuracy is greater than 1.3 mm, and the measurement error is less than 1.5 mm. The deformation monitoring system has the advantages of convenience, automation, and high accuracy and can be applied to ground deformation monitoring for subway structures.
Augmented Reality-Based BIM Data Compatibility Verification Method for FAB Digital Twin implementation
Authors: Song Jinwoo , Park Sanghyeon , Lee Kyuhyup , Bae Jinhyun , Kwon Soonwook , Cho Chung-Suk , Chung Suwan
Published: October 2023

With the advancement of state-of-the-art technologies, the semiconductor industry plays a key role as an essential component in the manufacture of various electronic products. Since the manufacturing of a semiconductor goes through very sophisticated and complex processes, efficient and accurate work and management are essential in the design, construction, and operation stages of the semiconductor fabrication (FAB) plant. Recently, the combined application of building information modeling (BIM) and augmented reality (AR) technology has gained increased attention in this semiconductor FAB industry as an advanced way to improve work efficiency and accuracy while eliminating other related problems, such as human errors. Despite the perceived benefits of combined use of BIM and AR, many technical problems still exist when integrating the target test model and the 3D virtual object model using BIM data and existing AR visualization technology, due to the unique characteristics of the FAB sites. To solve these problems, this study proposed an AR-based real-time BIM data compatibility verification method for future FAB digital twin implementation and demonstrated that it could be converted into a system and applied to actual FAB sites. As a result of the development and verification of this system, the proposed AR-based real-time BIM data compatibility verification system enables the accurate fitting of the AR model and actual object through AR tracking and anchoring technology considering the characteristics of FAB sites. After the fitting, the system was able to maintain compatibility, even when the camera moved and the marker moved away from the screen. By expanding the effective distance of compatibility between the AR model and the actual object, it was possible to increase the AR application range between the 3D virtual object model and the test target model and to improve the compatibility.
Hepatocellular Carcinoma: The Role of Immunotherapy and Transplantation in the Era of Transplant Oncology
Authors: Alghamdi Saad , Al-Hamoudi Waleed
Published: October 2023

Hepatocellular carcinoma (HCC) is one of the most common causes of cancer deaths worldwide. As most patients present with advanced disease, curative therapy such as surgical resection and radiofrequency ablation are rarely utilized. With the advent of immunotherapy, historical treatment approaches such as liver transplantation are being challenged. In particular, the use of immune checkpoint inhibitors (ICIs) has emerged as a safe and useful option in the treatment of HCC. However, there is concern over adverse effects, such as graft rejection and graft loss. This updated review discusses the role of immunotherapy in the pre- and post-transplantation setting and provides insights into the potential of immunotherapy as an adjunct to liver transplantation. We deliberate on the use of ICI in the setting of the Milan criteria as well as the University of California San Francisco’s expanded criteria for liver transplantation. Current data suggest that ICI has utility, especially in the pretransplantation setting. Nevertheless, larger, purposefully designed clinical trials are needed to clearly identify patients who will benefit most from ICI treatment in the transplant setting and determine parameters that will minimize the risk of graft rejection and maximize the benefits of this adjunct treatment.
Hepatocellular Carcinoma: Surveillance, Diagnosis, Evaluation and Management
Authors: Elderkin Jessica , Al Hallak Najeeb , Azmi Asfar S. , Aoun Hussein , Critchfield Jeffrey , Tobon Miguel , Beal Eliza W.
Published: October 2023

Hepatocellular carcinoma (HCC) ranks fourth in cancer-related deaths worldwide. Semiannual surveillance of the disease for patients with cirrhosis or hepatitis B virus allows for early detection with more favorable outcomes. The current underuse of surveillance programs demonstrates the need for intervention at both the patient and provider level. Mail outreach along with navigation provision has proven to increase surveillance follow-up in patients, while provider-targeted electronic medical record reminders and compliance reports have increased provider awareness of HCC surveillance. Imaging is the primary mode of diagnosis in HCC with The Liver Imaging Reporting and Data System (LI-RADS) being a widely accepted comprehensive system that standardizes the reporting and data collection for HCC. The management of HCC is complex and requires multidisciplinary team evaluation of each patient based on their preference, the state of the disease, and the available medical and surgical interventions. Staging systems are useful in determining the appropriate intervention for HCC. Early-stage HCC is best managed by curative treatment modalities, such as liver resection, transplant, or ablation. For intermediate stages of the disease, transarterial local regional therapies can be applied. Advanced stages of the disease are treated with systemic therapies, for which there have been recent advances with new drug combinations. Previously sorafenib was the mainstay systemic treatment, but the recent introduction of atezolizumab plus bevacizumab proves to have a greater impact on overall survival. Although there is a current lack of improved outcomes in Phase III trials, neoadjuvant therapies are a potential avenue for HCC management in the future.
Symptom Clusters in Survivorship and Their Impact on Ability to Work among Cancer Survivors
Authors: Fardell Joanna E. , Tan Sim Yee (Cindy) , Kerin-Ayres Kim , Dhillon Haryana M. , Vardy Janette L.
Published: October 2023

Background: Cancer survivors often experience a range of symptoms after treatment which can impact their quality of life. Symptoms may cluster or co-occur. We aimed to investigate how symptoms and symptom clusters impact the ability to work among cancer survivors. Methods: We used symptom severity data and ability to work data routinely collected from cancer survivors attending a survivorship clinic after primary treatment with curative intent. We defined symptom clusters using single linkage and a threshold on the rescaled distances of <10. We then conducted a logistic regression to examine how symptoms and symptom clusters were related to the ability to work. Results: We analysed data from 561 cancer survivors, mean age 58 years and 1.5 years post diagnosis, with mixed diagnoses including breast (40.5%), colorectal (32.3%), and haematological cancers (15.3%). Limitations to work ability were reported by 34.9% of participants. Survivors experiencing pain, emotional, and cognitive symptom clusters were 14–17% more likely to report limitations in their ability to work. Older survivors and those with a higher stage disease were more likely to report limitations in their ability to work. Conclusion: A better understanding and management of symptom severity and symptom clusters may help the sizable proportion of cancer survivors experiencing symptoms to participate in work after treatment.
Impacts of Dietary Selenium Nanoparticles from Spirulina platensis on Growth Performance, Physio-Biochemical Components and Alleviating Effect against Cadmium Toxicity in Pacific White Shrimp Litopenaeus vannamei
Authors: Said Radwa M. , Nassar Safaa E. , Alaidaroos Bothaina A. , Jastaniah Samyah D. , Dighiesh Hagar Sedeek , Eissa El-Sayed Hemdan , AL-Farga Ammar , Kari Zulhisyam Abdul , Téllez-Isaías Guillermo , Attia Mai S.
Published: October 2023

Shrimp culture is quite important and popular across the world. This study aimed to evaluate the growth-promoting potential of synthesized selenium nanoparticles from Spirulina platensis extract (SP-SeNPs) as a food source for Pacific whiteleg shrimp (Litopenaeus vannamei). However, pollution is considered a significant element affecting shrimp health and development. The effectiveness of SP-SeNPs in alleviating the negative effects of cadmium toxicity was also evaluated. Firstly, the shrimps (about 120 individuals with 6.0 ± 0.12 g of initial weight) were divided randomly into four groups in triplicates (30 shrimps/ treatment). The control group (SP-SeNPs—0 mg/kg diet) and three treatments were fed dietary SP-SeNPs (0.250, 0.50, and 1.0 mg/kg diet) for 56 days. Growth performance, digestive enzymes activities (protease, amylase, and lipase), and other biochemical components (total protein, lipid, amino acids, and carbohydrate) were evaluated. After 56 days of growth, another 150 adult shrimps were used under laboratory conditions to determine median lethal concentration of cadmium (96 h LC50), and 30 individuals were treated with cadmium (1/2 of LC50, 0.2 mg L−1) for 10 days only. Tissue samples were collected for measuring catalase (CAT), total antioxidant capacity (TAC), superoxide dismutase (SOD), malondialdehyde (MDA) levels, cadmium bioaccumulation, and histopathological investigation. The results illustrated that the application of SP-SeNPs as feed additives at varying levels significantly improved growth performance (high weight gain, specific growth rate, and low feed conversion rates) relative to the control group. Furthermore, dietary SP-SeNPs enhanced digestive enzyme activities and the concentrations of biochemical components more than the control group. Upon concurrent exposure to cadmium, the antioxidative status was significantly enhanced, and histopathological alterations were mitigated. In conclusion, this study recommended supplementation of SP-SeNPs at 0.50 mg/kg diet to enhance optimal growth rate, digestive enzyme activities, levels of antioxidants in Litopenaeus vannamei, and mitigate the pathological alternations induced with Cd toxicity.
SUMOylation Modulates Reactive Oxygen Species (ROS) Levels and Acts as a Protective Mechanism in the Type 2 Model of Diabetic Peripheral Neuropathy
Authors: Mandel Nicolas , Büttner Michael , Poschet Gernot , Kuner Rohini , Agarwal Nitin
Published: October 2023

Diabetic peripheral neuropathy (DPN) is the prevalent type of peripheral neuropathy; it primarily impacts extremity nerves. Its multifaceted nature makes the molecular mechanisms of diabetic neuropathy intricate and incompletely elucidated. Several types of post-translational modifications (PTMs) have been implicated in the development and progression of DPN, including phosphorylation, glycation, acetylation and SUMOylation. SUMOylation involves the covalent attachment of small ubiquitin-like modifier (SUMO) proteins to target proteins, and it plays a role in various cellular processes, including protein localization, stability, and function. While the specific relationship between high blood glucose and SUMOylation is not extensively studied, recent evidence implies its involvement in the development of DPN in type 1 diabetes. In this study, we investigated the impact of SUMOylation on the onset and progression of DPN in a type 2 diabetes model using genetically modified mutant mice lacking SUMOylation, specifically in peripheral sensory neurons (SNS-Ubc9−/−). Behavioural measurement for evoked pain, morphological analyses of nerve fibre loss in the epidermis, measurement of reactive oxygen species (ROS) levels, and antioxidant molecules were analysed over several months in SUMOylation-deficient and control mice. Our longitudinal analysis at 30 weeks post-high-fat diet revealed that SNS-Ubc9−/− mice exhibited earlier and more pronounced thermal and mechanical sensation loss and accelerated intraepidermal nerve fibre loss compared to control mice. Mechanistically, these changes are associated with increased levels of ROS both in sensory neuronal soma and in peripheral axonal nerve endings in SNS-Ubc9−/− mice. In addition, we observed compromised detoxifying potential, impaired respiratory chain complexes, and reduced levels of protective lipids in sensory neurons upon deletion of SUMOylation in diabetic mice. Importantly, we also identified mitochondrial malate dehydrogenase (MDH2) as a SUMOylation target, the activity of which is negatively regulated by SUMOylation. Our results indicate that SUMOylation is an essential neuroprotective mechanism in sensory neurons in type 2 diabetes, the deletion of which causes oxidative stress and an impaired respiratory chain, resulting in energy depletion and subsequent damage to sensory neurons.
Health-Related Quality of Life and Mental Health of Children with Embryonal Abdominal Tumors
Authors: Behrendt Paulina , Boettcher Michael , Zierke Kira Tabea , Najem Safiullah , Zapf Holger , Reinshagen Konrad , Wößmann Wilhelm , Boettcher Johannes
Published: October 2023

(1) Background: Embryonal abdominal tumors are one of the most common entities of solid childhood cancer. The present study investigates the Health-Related Quality of Life (HRQoL) and the mental health of children to obtain a comprehensive picture of their health status and uncover a possible gap in healthcare. (2) Methods: The sample consisted of 54 children who were treated for embryonal abdominal tumors and a control group of 46 children who received uncomplicated outpatient surgery. The HRQoL and the mental health were assessed by the parent proxy reports of the questionnaires Pediatric Quality of Life Inventory (PedsQL) and Strengths and Difficulties Questionnaire (SDQ). (3) Results: Children with embryonal abdominal tumors showed significantly lower HRQoL and mental health values compared to the norm data. The index group showed lower values in the social subscales of HRQoL and mental health compared to the control group. (4) Conclusions: Embryonal abdominal tumors affect the well-being of children. There is still a gap in healthcare due to children’s HRQoL and mental health, especially regarding social development. It is essential to further advance the psychological care of children and improve their chances to develop social relationships.
Comparison of Cobalt Integration with Various Dielectric Materials under Thermal and Electrical Stress
Authors: Cheng Yi-Lung , Huang Hong-Chang , Peng Wei-Fan , Chen Giin-Shan , Fang Jau-Shiung
Published: October 2023

Cobalt (Co) is proposed to replace copper (Cu) as a conductor in the back-end-of-line (BEOL) interconnects of integrated circuits. In this study, the electric characteristics and reliability of the integration of Co with various dielectric films (SiO2, dense, and porous low-k SiOCH films) under thermal and electrical stress were compared. Thermal annealing repaired sputtering-Co-deposition-induced damage to the dielectric film but reduced the breakdown field and time-dependence-dielectric-breakdown (TDDB) times due to the diffusion of Co atoms. After annealing, the SiO2 film had the largest reductions in the breakdown field, TDDB failure time, and electric field acceleration factor, indicating that the diffusion of Co atoms dominates in the oxygen-rich surface. Under electrical stress, the drift of Co atoms favors the porous low-k film with the assistance of porosity. As a result, a barrier is required for Co metallization. To achieve barrier-free or barrier-less processing in Co metallization, the dense low-k film is the best option to integrate with Co.
Titanium–Platinum Thin Films as a Tool for the Electrooxidation of Cyanide
Authors: Valiūnienė Aušra , Virbickas Povilas , Gabriunaite Inga , Margarian Zana , Levinas Ramūnas , Janarauskiene Dagne , Valincius Gintaras
Published: October 2023

This paper presents a detailed study of a titanium–platinum thin film-based electrode preparation and its practical application in the electrooxidation of cyanides to help protect our environment. The novel electrochemical deposition process of Pt on nearly atomically smooth magnetron-sputtered Ti film has been used to prepare a highly effective glass|Ti|Pt composite electrode with high catalytic activity for the electrooxidation of cyanide ions. The composite electrode exhibits over a 90% electrical current efficiency in the cyanide electrooxidation process and can be used for the decontamination of highly concentrated KCN solutions (up to 0.1 M) without any chemical additives. A high current efficiency (70%) of Pt thin film deposition on a glass|Ti electrode was achieved using a potentiostatic double-pulse method. Fast Fourier transform electrochemical impedance spectroscopy revealed the oxidation kinetics for cyanide ions at the electrode. The glass|Ti electrode was prepared using the magnetron sputtering technique, which allows us to fabricate electrodes of any shape suitable for any electrochemical cell or electroplating bath. Meanwhile, electrochemical deposition of Pt on the glass|Ti electrode is an efficient and environmentally friendly method, since various salts of Pt and/or Pt-containing wastes can be used for electrodeposition instead of pure Pt, which is more expensive.
Effect of Surface Modification via Laser Irradiation on the Operability of Carbide End Mills When Cutting Aircraft Alloys
Authors: Gusarov Andrey V. , Migranov Mars S. , Mitrofanov Artem P. , Gusev Andrey S. , Migranov Artur M. , Khmyrov Roman S.
Published: October 2023

In modern aviation production, innovative hard-to-machine materials with unique physical and mechanical properties are being used increasingly. When processing such materials, the weakest link in the technological chain of production is the metal-cutting tool. In this paper, to improve the efficiency of the blade cutting of heat-resistant alloys, we propose the use of nanostructured multilayer wear-resistant coatings with subsequent laser processing of the cutting surfaces of the end milling cutters according to various schemes. In this case, an increase in the wear-resistant properties of the cutting edge by 15%–20% is provided due to the formation, at high temperatures, of secondary structures with increased wear resistance and a reduction of the temperature and force loading of contact processes. Methodologically, the work was carried out in several consecutive stages: the first stage was the determination of effective grades of wear-resistant coatings obtained via various installations with their subsequent laser processing during the «SharpMark™ Fiber» installation; at the second stage tribotechnical tests were carried out during the tribometer and adhesion installation; and in the third stage wear-resistant, temperature-force tests were carried out using milling machines in various cutting modes. According to the results of the field tests, the tool durability period was increased by 15%–20%.
New Insights into the Coloration Mechanism in Spessartines and the Impact of Munsell Neutral Grey Backgrounds
Authors: Zhu Meilin , Guo Ying
Published: October 2023

In this study about spessartines,13 spessartine samples with proper conditions are used for FTIR spectrum and UV-Vis spectrum tests to find the gemological characteristics and coloration mechanism. In the UV-Vis spectrum, spessartines’ special color has a relationship with the absorption bands at about 460 nm and 480 nm. Based on the CIE 1976 L*a*b* colour system, we come to the conclusion that both color coordinates a* and b* control the value of chroma C*, and color coordinate b* mostly controls the hue angle h°. We also explore how the different chromogenic ions FeOtot and MnO and their ratio FeOtot/MnO influence spessartines’ color, finding that the color of spessartine samples is influenced by both Mn and Fe. By analyzing the FTIR spectrum, we discovered that as the content of Mn decreases, and the A, C and D peaks move to the position of a longer wave, the color of spessartine samples also changes significantly. By using the standard light source D65, we find that N9.5 Neutral Grey Background is the best background to grade the color of spessartines.
Introduction of Androgen Receptor Targeting shRNA Inhibits Tumor Growth in Patient-Derived Prostate Cancer Xenografts
Authors: Thomas Patrick B. , Alinezhad Saeid , Joshi Andre , Sweeney Katrina , Tse Brian W. C. , Tevz Gregor , McPherson Stephen , Nelson Colleen C. , Williams Elizabeth D. , Vela Ian
Published: October 2023

Patient-derived xenograft (PDX) models have been established as important preclinical cancer models, overcoming some of the limitations associated with the use of cancer cell lines. The utility of prostate cancer PDX models has been limited by an inability to genetically manipulate them in vivo and difficulties sustaining PDX-derived cancer cells in culture. Viable, short-term propagation of PDX models would allow in vitro transfection with traceable reporters or manipulation of gene expression relevant to different studies within the prostate cancer field. Here, we report an organoid culture system that supports the growth of prostate cancer PDX cells in vitro and permits genetic manipulation, substantially increasing the scope to use PDXs to study the pathobiology of prostate cancer and define potential therapeutic targets. We have established a short-term PDX-derived in vitro cell culture system which enables genetic manipulation of prostate cancer PDXs LuCaP35 and BM18. Genetically manipulated cells could be re-established as viable xenografts when re-implanted subcutaneously in immunocompromised mice and were able to be serially passaged. Tumor growth of the androgen-dependent LuCaP35 PDX was significantly inhibited following depletion of the androgen receptor (AR) in vivo. Taken together, this system provides a method to generate novel preclinical models to assess the impact of controlled genetic perturbations and allows for targeting specific genes of interest in the complex biological setting of solid tumors.
Correlation between Statin Solubility and Mortality in Patients on Chronic Hemodialysis
Authors: Kang Seok-Hui , Kim Gui-Ok , Kim Bo-Yeon , Son Eun-Jung , Do Jun-Young
Published: October 2023

This study aimed to evaluate the effect of statin solubility on the survival of patients undergoing hemodialysis (HD). This retrospective study used laboratory and clinical data from a national HD quality assessment program and claims data (n = 53,345). The use of statins was defined as prescription ≥30 days during 6 months of each HD quality assessment period. We divided the patients into three groups based on the use and solubility of statins: No group, patients without a prescription of statins (n = 37,944); Hydro group, patients with a prescription of hydrophilic statins (n = 2823); and Lipo group, patients with a prescription of lipophilic statins (n = 12,578). The 5-year survival rates in the No, Hydro, and Lipo groups were 69.6%, 67.9%, and 67.9%, respectively (p < 0.001 for the trend). Multivariable Cox regression analyses showed that the Lipo group had better patient survival than the No group. However, multivariable analyses did not show statistical significance between the Hydro and No or Lipo groups. In all subgroups based on sex, age, presence of diabetes mellitus, and heart disease, the Lipo group had better patient survival than the No group. We identified no significant association between hydrophilic and lipophilic statins and patient survival. However, patients taking lipophilic statins had a modest survival benefit compared with those who did not receive statins.
A Benchmark of In-House Homologous Recombination Repair Deficiency Testing Solutions for High-Grade Serous Ovarian Cancer Diagnosis
Authors: Guarischi-Sousa Rodrigo , Kroll José Eduardo , Bonaldi Adriano , Pierry Paulo Marques , Villela Darine , Souza Camila Alves , Silva Juliana Santos , Bürger Matheus Carvalho , Oliveira Felipe Azevedo , de Paula Marcelo Gomes , Meliso Fabiana Marcelino , de Almeida Luiz Gustavo , Monfredini Priscilla Morais , de Oliveira Ana Gabriela , Milanezi Fernanda , Scapulatempo-Neto Cristovam , Yamamoto Guilherme Lopes
Published: October 2023

Homologous recombination deficiency (HRD) has become an important prognostic and predictive biomarker for patients with high-grade serous ovarian cancer who may benefit from poly-ADP ribose polymerase inhibitors (PARPi) and platinum-based therapies. HRD testing provides relevant information to personalize patients’ treatment options and has been progressively incorporated into diagnostic laboratories. Here, we assessed the performance of an in-house HRD testing system deployable in a diagnostic clinical setting, comparing results from two commercially available next-generation sequencing (NGS)-based tumor tests (SOPHiA DDMTM HRD Solution and AmoyDx® (HRD Focus Panel)) with the reference assay from Myriad MyChoice® (CDx). A total of 85 ovarian cancer samples were subject to HRD testing. An overall strong correlation was observed across the three assays evaluated, regardless of the different underlying methods employed to assess genomic instability, with the highest pairwise correlation between Myriad and SOPHiA (R = 0.87, p-value = 3.39 × 10−19). The comparison of the assigned HRD status to the reference Myriad’s test revealed a positive predictive value (PPV) and negative predictive value (NPV) of 90.9% and 96.3% for SOPHiA’s test, while AmoyDx’s test achieved 75% PPV and 100% NPV. This is the largest HRD testing evaluation using different methodologies and provides a clear picture of the robustness of NGS-based tests currently offered in the market. Our data shows that the implementation of in-house HRD testing in diagnostic laboratories is technically feasible and can be reliably performed with commercial assays. Also, the turnaround time is compatible with clinical needs, making it an ideal alternative to offer to a broader number of patients while maintaining high-quality standards at more accessible price tiers.