bluuebunny/crossref_metadata_2025 huggingface dataset:
Higher Adherence to Plant-Based Diet Lowers Type 2 Diabetes Risk among High and Non-High Cardiovascular Risk Populations: A Cross-Sectional Study in Shanxi, China
Authors: Zhang Ying , Meng Yaqing , Wang Junbo
Published: February 2023

This study aimed to investigate the association between the plant-based diet index (PDI) score and T2D risk among residents of Shanxi Province, China, and explore whether the association was influenced by different levels of cardiovascular risk. A total of 50,694 participants aged 35–75 years were recruited between 2017 and 2019, and they were further divided into the high cardiovascular risk population (HCRP; n = 17,255) and the non-high cardiovascular risk population (non-HCRP; n = 33,439). The PDI was calculated based on food frequency from a food frequency questionnaire (FFQ). Incident T2D was defined based on elevated plasma glucose (≥7 mmol/L) or hypoglycemic medicine use. We investigated the association of the PDI andT2D risk using a two-level generalized estimating equation and restricted cubic splines model. The results showed that quartile 4 of the PDI indicated significantly reduced T2D risk in the total population (OR: 0.83; 95% CI: 0.75–0.92), HCRP (OR: 0.80; 95% CI: 0.71–0.91), and non-HCRP (OR: 0.80; 95% CI: 0.74–0.87) compared with corresponding quartile 1 (OR = 1). In stratified analysis, the negative associations between PDI and T2D risk were stronger in the total population with the elderly (age > 60 years), BMI < 24, and men, and in the non-HCRP with men and BMI 24–28, and in the HCRP with the elderly and BMI < 24 than those with corresponding subgroups (pinteraction < 0.05). Linear curves were observed for the total population and non-HCRP, but an L-shaped association was observed for the HCRP. Therefore, our results suggest that higher PDI scores may effectively attenuate the T2D risk in the Chinese population and non-HCRP, and a beneficial association of PDI with T2D risk was observed in the HCRP at a certain threshold level. Longitudinal studies and intervention trials are required to validate our study findings.
Dietary Supplementation of Methyl Cedryl Ether Ameliorates Adiposity in High-Fat Diet-Fed Mice
Authors: Li Mengjie , Kang Seong-Gook , Huang Kunlun , Tong Tao
Published: February 2023

Methyl cedryl ether (MCE) is a derivative of cedrol and is widely used as a fragrance compound. The aim of this study was to evaluate the preventative effects of MCE on obesity and related metabolic syndromes and to delineate the mechanisms from the perspective of gut microbiota and white adipose tissues (WAT) transcriptomic profiles. Five-week-old male C57BL/6J mice were randomly assigned into 3 groups and fed with chow diet, high-fat diet (HFD), or HFD supplemented with 0.2% (w/w) MCE for 13 weeks. We found that MCE significantly reduced body weight, inhibited adipocyte hypertrophy, and ameliorated hepatic steatosis under HFD conditions. MCE dietary supplementation downregulated the expression of adipogenesis genes (FAS and C/EBPα) and upregulated the mRNA levels of thermogenesis genes (PGC-1α, PRDM16, UCP1, Cidea, Cytc, and COX4) in epididymal WAT. 16S rRNA sequencing revealed that MCE improved gut microbiota dysbiosis in HFD-fed mice, as manifested by the alteration of strains associated with obesity. Further transcriptome analysis of WAT indicated that MCE dramatically changed the gene expression profiles. Our results demonstrate the anti-obesity effect of MCE under HFD conditions, highlighting the nutraceutical potential of MCE for preventing obesity.
Masses of Compact (Neutron) Stars with Distinguished Cores
Authors: Zöllner Rico , Ding Minghui , Kämpfer Burkhard
Published: February 2023

In this paper, the impact of core mass on the compact/neutron-star mass-radius relation is studied. Besides the mass, the core is parameterized by its radius and surface pressure, which supports the outside one-component Standard Model (SM) matter. The core may accommodate SM matter with unspecified (or poorly known) equation-of-state or several components, e.g., consisting of admixtures of Dark Matter and/or Mirror World matter etc. beyond the SM. Thus, the admissible range of masses and radii of compact stars can be considerably extended.
COVID-19 among People Living with HTLV-1 Infection in Rio de Janeiro, Brazil
Authors: Puccioni-Sohler Marzia , Miranda Alana Cristina Jasset , da Silva Mello Cíntia , Magalhães Stéphanie Monnerat , dos Santos Rodrigues Luciane Cardoso , Signorini Dario J. H. P.
Published: February 2023

The impact of coronavirus disease 2019 (COVID-19) on people living with human T-cell leukemia virus type 1 (HTLV-1) is unknown. The aim of this study is to evaluate the COVID-19 risk factors and outcomes of HTLV-1-infected individuals. A retrospective study of seropositive HTLV-1 outpatients seen during the COVID-19 pandemic period (2020–2022) was conducted in a Tertiary Hospital in Rio de Janeiro, Brazil. We compared the demographic and comorbidity/risk factors in patients with COVID-19 and non-COVID-19 diagnoses. In addition, the clinical features of COVID-19 and vaccination status were also investigated in 51 HTLV-1-infected individuals. The majority (88.2%) had COVID-19 comorbidity/risk factors. Seven cases were vaccinated against COVID-19. Overall, 19 out of 51 (37.3%) individuals were diagnosed with COVID-19. We found differences only in the frequency of anxiety in both groups: 57.9% in the COVID-19 group vs. 15.6% in the non-COVID-19 (p < 0.05) group. Thirteen out of nineteen (68%) of the COVID-19 cases progressed to mild/moderate illness, one remained asymptomatic, and 26.3% progressed to severe illness. All of the individuals recovered at home, but the majority (57.9%) developed post-COVID-19 symptoms: anosmia and ageusia (31.6%), worsening anxiety (15.8%), and a feeling of pain in the legs (15.8%). The patients with post-COVID-19 conditions were unvaccinated. Our findings show that HTLV-1 did not increase the risk of lethal COVID-19 and underline the importance of promoting mental health in HTLV-1-infected individuals.
Structures of Mammeasins P and Q, Coumarin-Related Polysubstituted Benzofurans, from the Thai Medicinal Plant Mammea siamensis (Miq.) T. Anders.: Anti-Proliferative Activity of Coumarin Constituents against Human Prostate Carcinoma Cell Line LNCaP
Authors: Luo Fenglin , Manse Yoshiaki , Chaipech Saowanee , Pongpiriyadacha Yutana , Muraoka Osamu , Morikawa Toshio
Published: February 2023

A methanol extract of the flowers of Mammea siamensis (Miq.) T. Anders. (Calophyllaceae) showed anti-proliferative activity against human prostate carcinoma LNCaP cells (IC50 = 2.0 µg/mL). Two new coumarin-related polysubstituted benzofurans, mammeasins P (1) and Q (2), and a known polysubstituted coumarin mammea B/AC cyclo F (39) were isolated from the extract along with 44 previously reported polysubstituted coumarin constituents (3–38 and 40–47). The structures of two new compounds (1 and 2) were determined based on their spectroscopic properties derived from the physicochemical evidence including NMR and MS analyses and taking the plausible generative pathway into account. Among the coumarin constituents, mammeasins A (3, IC50 = 1.2 µM) and B (4, 0.63 µM), sugangin B (18, 1.5 µM), kayeassamins E (24, 3.0 µM) and G (26, 3.5 µM), and mammeas E/BA (40, 0.88 µM), E/BB (41, 0.52 µM), and E/BC (42, 0.12 µM) showed relatively potent anti-proliferative activity.
Chitosan-Based Nanoparticles with Optimized Parameters for Targeted Delivery of a Specific Anticancer Drug—A Comprehensive Review
Authors: Kurczewska Joanna
Published: February 2023

Chitosan is a positively charged polysaccharide obtained through chitin deacetylation. It belongs to a group of biodegradable, bioavailable, and non-toxic materials of natural origin; thus, it is a promising matrix for creating delivery systems of different active agents. Recently, much attention has been paid to nanodelivery systems as carriers to enable better bioavailability, and thus higher efficiency of the loaded drug. The present review is focused on the progress in chitosan-based nanoparticles for the targeted delivery of antitumor drugs. The paper discusses literature reports from the last three years in which chitosan nanoparticles were applied as carriers for active substances used in antitumor therapy and potential new drugs with anticancer properties. Special attention was paid to the different treatments applied to increase the therapeutic effectiveness and minimize the side effects of a specific active substance.
Sustained Release of Tacrolimus Embedded in a Mixed Thermosensitive Hydrogel for Improving Functional Recovery of Injured Peripheral Nerves in Extremities
Authors: Wang Aline Yen Ling , Chen Kuan-Hung , Lin Hsiu-Chao , Loh Charles Yuen Yung , Chang Yun-Ching , Aviña Ana Elena , Lee Chin-Ming , Chu I-Ming , Wei Fu-Chan
Published: February 2023

Vascularized composite allotransplantation is an emerging strategy for the reconstruction of unique defects such as amputated limbs that cannot be repaired with autologous tissues. In order to ensure the function of transplanted limbs, the functional recovery of the anastomosed peripheral nerves must be confirmed. The immunosuppressive drug, tacrolimus, has been reported to promote nerve recovery in animal models. However, its repeated dosing comes with risks of systemic malignancies and opportunistic infections. Therefore, drug delivery approaches for locally sustained release can be designed to overcome this issue and reduce systemic complications. We developed a mixed thermosensitive hydrogel (poloxamer (PLX)-poly(l-alanine-lysine with Pluronic F-127) for the time-dependent sustained release of tacrolimus in our previous study. In this study, we demonstrated that the hydrogel drug degraded in a sustained manner and locally released tacrolimus in mice over one month without affecting the systemic immunity. The hydrogel drug significantly improved the functional recovery of injured sciatic nerves as assessed using five-toe spread and video gait analysis. Neuroregeneration was validated in hydrogel–drug-treated mice using axonal analysis. The hydrogel drug did not cause adverse effects in the mouse model during long-term follow-up. The local injection of encapsulated-tacrolimus mixed thermosensitive hydrogel accelerated peripheral nerve recovery without systemic adverse effects.
Immunotherapy with Cleavage-Specific 12A12mAb Reduces the Tau Cleavage in Visual Cortex and Improves Visuo-Spatial Recognition Memory in Tg2576 AD Mouse Model
Authors: Latina Valentina , De Introna Margherita , Caligiuri Chiara , Loviglio Alessia , Florio Rita , La Regina Federico , Pignataro Annabella , Ammassari-Teule Martine , Calissano Pietro , Amadoro Giuseppina
Published: February 2023

Tau-targeted immunotherapy is a promising approach for treatment of Alzheimer’s disease (AD). Beyond cognitive decline, AD features visual deficits consistent with the manifestation of Amyloid β-protein (Aβ) plaques and neurofibrillary tangles (NFT) in the eyes and higher visual centers, both in animal models and affected subjects. We reported that 12A12—a monoclonal cleavage-specific antibody (mAb) which in vivo neutralizes the neurotoxic, N-terminal 20–22 kDa tau fragment(s)–significantly reduces the retinal accumulation in Tg(HuAPP695Swe)2576 mice of both tau and APP/Aβ pathologies correlated with local inflammation and synaptic deterioration. Here, we report the occurrence of N-terminal tau cleavage in the primary visual cortex (V1 area) and the beneficial effect of 12A12mAb treatment on phenotype-associated visuo-spatial deficits in this AD animal model. We found out that non-invasive administration of 12 A12mAb markedly reduced the pathological accumulation of both truncated tau and Aβ in the V1 area, correlated to significant improvement in visual recognition memory performance along with local increase in two direct readouts of cortical synaptic plasticity, including the dendritic spine density and the expression level of activity-regulated cytoskeleton protein Arc/Arg3.1. Translation of these findings to clinical therapeutic interventions could offer an innovative tau-directed opportunity to delay or halt the visual impairments occurring during AD progression
Comparison of the In Vitro Drug Release Methods for the Selection of Test Conditions to Characterize Solid Lipid Microparticles
Authors: Wolska Eliza , Szymańska Martyna
Published: February 2023

The release profiles of active substances from microspheres are one of the most important features in solid lipid microparticles (SLM) characterization. Unfortunately, the results of the dissolution tests are largely dependent on the chosen method and test conditions, which in relation to novel dosage forms, such as dispersions of lipid microspheres, are not clearly defined in international compendiums and guidelines. This makes it impossible to compare the results of different studies. The aim of the research was to identify the factors most influencing the variability of the obtained results. An attempt was also made to select the most appropriate method for testing drug substance release from SLM. Various dissolution methods were employed (method I: without a membrane, method II: in a dialysis bag, and method III: in a Side-Bi-Side chamber), and the obtained release profiles of cyclosporine and indomethacin from SLM dispersions were compared. In addition to the effect of membranes, the types of acceptor fluids were also investigated. Significant differences were observed when testing the SLM formulations under various test conditions. The results were significantly influenced by the selected membrane, the acceptor fluid, or the difference in the concentrations of active substance between the donor and acceptor compartments. The burst effect observed in some experimental methods was not noticed in other conditions. At this stage, the method with a dialysis bag has been selected as the most suitable, while the methods without the membrane can only play a complementary role.
Montmorillonite–Rifampicin Nanohybrid for pH-Responsive Release of the Tuberculostatic
Authors: Damasceno Junior Elmar , Barbosa Raquel de Melo , da Silva Rita de Cássia Dantas , Costa Felipe dos Santos , da Silva Djalma Ribeiro , Viseras César , Perioli Luana , Fernandes Nedja Suely
Published: February 2023

The present work describes the development of a hybrid and pH-responsive system for rifampicin using the clay mineral ‘montmorillonite’ as a nanocarrier. The influence of operational variables on the drug incorporation process was evaluated using 24 factorial designs. Under optimized conditions, the experiment allowed an incorporated drug dose equivalent to 98.60 ± 1.21 mg/g. Hybrid systems were characterized by different characterization techniques (FTIR, XRD, TGA, DSC, and SEM) to elucidate the mechanism of interaction between the compounds used. Through in vitro release studies, it was possible to verify the efficacy of the pH-dependent system obtained, with approximately 70% of the drug released after sixteen hours in simulated intestinal fluid. The adjustment of the experimental release data to the theoretical model of Higuchi and Korsmeyer–Peppas indicated that the release of rifampicin occurs in a prolonged form from montmorillonite. Elucidation of the interactions between the drug and this raw clay reinforces its viability as a novel carrier to develop an anti-TB/clay hybrid system with good physical and chemical stability.
Quality by Design (QbD) Approach for a Nanoparticulate Imiquimod Formulation as an Investigational Medicinal Product
Authors: Pielenhofer Jonas , Meiser Sophie Luise , Gogoll Karsten , Ciciliani Anna-Maria , Denny Mark , Klak Michael , Lang Berenice M. , Staubach Petra , Grabbe Stephan , Schild Hansjörg , Radsak Markus P. , Spahn-Langguth Hilde , Langguth Peter
Published: February 2023

The present article exemplifies the application of the concept of quality by design (QbD) for the systematic development of a nanoparticulate imiquimod (IMQ) emulsion gel formulation as an investigational medicinal product (IMP) for evaluation in an academic phase-I/II clinical trial for the treatment of actinic keratosis (AK) against the comparator Aldara (EudraCT: 2015-002203-28). The design of the QbD elements of a quality target product profile (QTPP) enables the identification of the critical quality attributes (CQAs) of the drug product as the content of IMQ, the particle-size distribution, the pH, the rheological properties, the permeation rate and the chemical, physical and microbiological stability. Critical material attributes (CMAs) and critical process parameters (CPPs) are identified by using a risk-based approach in an Ishikawa diagram and in a risk-estimation matrix. In this study, the identified CPPs of the wet media ball-milling process’s milling time and milling speed are evaluated in a central composite design of experiments (DoEs) approach, revealing criticality for both factors for the resulting mean particle size, while only the milling time is significantly affecting the polydispersity. To achieve a mean particle size in the range of 300–400 nm with a minimal PdI, the optimal process conditions are found to be 650 rpm for 135 min. Validating the model reveals a good correlation between the predicted and observed values. Adequate control strategies were implemented for intermediate products as in-process controls (IPCs) and quality control (QC) tests of the identified CQAs. The IPC and QC data from 13 “IMI-Gel” batches manufactured in adherence to good manufacturing practice (GMP) reveal consistent quality with minimal batch-to-batch variability.
Current Applications and New Perspectives in Optical Coherence Tomography (OCT) Coronary Atherosclerotic Plaque Assessment: From PCI Optimization to Pharmacological Treatment Guidance
Authors: Viscusi Michele Mattia , La Porta Ylenia , Migliaro Giuseppe , Gargano Gian Marco , Nusca Annunziata , Gatto Laura , Budassi Simone , Paolucci Luca , Mangiacapra Fabio , Ricottini Elisabetta , Melfi Rosetta , Rinaldi Raffaele , Prati Francesco , Ussia Gian Paolo , Grigioni Francesco
Published: February 2023

Since its ability to precisely characterized atherosclerotic plaque phenotypes, to tailor stent implantation, as well as to guide both complex percutaneous coronary interventions (PCI) and invasive diagnostic work-ups (e.g., spontaneous coronary dissections or myocardial infarction with non-obstructive arteries), the adoption of optical coherence tomography (OCT) was raised in the past decades in order to provide complementary information to the traditional angiography and to overcome its limitations. However, the impact of OCT on daily clinical practice is currently modest, firstly because of the lack of both standardized algorithms of PCI guidance and data from prospective clinical trials. Therefore, the aim of our narrative review is to provide a comprehensive overview of the basic OCT interpretation, to summarize the evidence supporting the OCT guidance procedures and applications, to discuss its current limitations, and to highlight the knowledge gaps that need to be filled with more robust evidence.
Conversion and Active Control between Electromagnetic Induced Transparency and Absorber in Terahertz Metasurface
Authors: Zhang Yuting , Jing Benqin , Liu Songyi , Hao Xiaoyuan , Luo Zhongyue , Zou Jinhua , Yin Shan , Huang Wei , Zhang Wentao
Published: February 2023

In this study, we use a phase-changing material vanadium dioxide (VO2) to design a multilayer metasurface structure to achieve the transition from an electromagnetically induced transparency(EIT) device to an absorber. The structure consists of a gold layer, a polyimide spacer layer, a VO2 layer, and a sapphire substrate. The top layer consists of one cut wire and two split-ring resonators with the same parameters. When the VO2 layer is in its insulating phase at room temperature, the peak of the EIT device will appear near 1.138 THz. When the VO2 layer is in the metallic state, two absorption peaks above 99.5% appear separately at 1.19 and 1.378 THz, respectively. To the best of our knowledge, this is the first time that a coupled mode equation is used to perform theoretical calculations for EIT devices and perfect absorbers simultaneously, and this is also the first time that coupled mode equations are used for the theoretical calculations of two absorption peaks in an absorber. The proposed metasurface combines the advantages of terahertz absorption, EIT and active device control, which will provide more ideas for the design of future terahertz devices and is also significant for the design and development of radomes for future stealth aircraft.
Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
Authors: Al-Rahbi Buthaina Aamir Ali , Al-Sadi Abdullah Mohammed , Al-Harrasi Majida Mohammed Ali , Al-Sabahi Jamal Nasser , Al-Mahmooli Issa Hashil , Blackburn Daniel , Velazhahan Rethinasamy
Published: February 2023

In this study, endophytic and rhizospheric bacteria were isolated from Moringa olifera and M. perigreina from Oman, and their in vitro antagonistic activity against Pythium aphanidermatum was tested using a dual culture assay. The promising strains were tested further for their compatibility and potential for plant growth promotion, biofilm formation, antifungal volatile organic compound (VOC) production, and the biological control of P. aphanidermatum damping-off of cabbage (Brassica oleracea L.) under greenhouse conditions. A total of 12 endophytic and 27 rhizospheric bacteria were isolated from Moringa spp. Among them, Bacillus pumilus MPE1 showed the maximum antagonistic activity against P. aphanidermatum in the dual culture assay, followed by Paenibacillus glucanolyticus MPE3 and Pseudomonas indica MOR3 and MOR8. These bacterial isolates induced abundant morphological abnormalities in the hyphae of P. aphanidermatum, as observed via scanning electron microscopy. The in vitro cross-streak assay showed that these bacterial isolates were compatible among themselves, except for P. indica MOR8 × P. glucanolyticus MPE3. These antagonists released VOCs that restricted the growth of P. aphanidermatum in an in vitro assay. These antagonistic bacteria released 2,4-dimethylheptane and 4-methyloctane as the predominant volatile compounds. Of the four antagonistic bacterial strains, P. indica MOR8 was capable of forming biofilm, which is considered a trait that improves the efficacy of rhizosphere inoculants. The results of the greenhouse experiments showed that the soil treatment with B. pumilus MPE1 showed the highest reduction (59%) in the incidence of P. aphanidermatum damping-off in cabbage, evidencing its potential as a biological control agent for the management of this disease. Further research is needed to characterize the antifungal traits and activities of B. pumilus MPE1 and to assert its potential use against other soil-borne plant pathogens.
The Interactive Effects of Deficit Irrigation and Bacillus pumilus Inoculation on Growth and Physiology of Tomato Plant
Authors: Liu Jie , Zhang Jiarui , Shi Qimiao , Liu Xiangliang , Yang Zhen , Han Pan , Li Jingjing , Wei Zhenhua , Hu Tiantian , Liu Fulai
Published: February 2023

The effects of inoculating plant growth promoting rhizobacteria (PGPR) and soil water deficits on crop growth and physiology remain largely unknown. Here, the responses of leaf gas exchange, growth, and water use efficiency (WUE) of tomato plants to Bacillus pumilus (B.p.) inoculation under four irrigation strategies (I1-I4) were investigated in a greenhouse. Results showed that soil water deficits, especially at I4 (20%, v/v), significantly decreased leaf stomatal conductance (gs), transpiration rate (Tr), and photosynthetic rate (An), and the decrease of gs and Tr were more pronounced than An. Reduced irrigation regimes significantly lowered dry matter and plant water use both in the non-B.p. control and the B.p. plants, while reduced irrigation significantly increased plant WUE, and B.p. inoculation had little effect on this parameter. Synergistic effects of PGPR and deficit irrigation on leaf gas exchange, leaf abscisic acid content, and stomatal density were found in this study, and specifically, B.p. treated plants at I4 possessed the highest WUE at stomatal and leaf scales, suggesting that B.p. inoculation could optimize water use and partly alleviate the negative effects of soil water deficit. These findings provide useful information for effective irrigation management and the application of PGPR in agriculture in the future.
Pollination in Epidendrum densiflorum Hook. (Orchidaceae: Laeliinae): Fraudulent Trap-Flowers, Self-Incompatibility, and a Possible New Type of Mimicry
Authors: Silveira Rodrigo Santtanna , Singer Rodrigo Bustos , Ferro Viviane Gianluppi
Published: February 2023

The pollination and the breeding system of Epidendrum densiflorum (Orchidaceae: Laeliinae) were studied through fieldwork and controlled pollinations in cultivated plants. Pollination is exclusively promoted by males of diurnal Lepidoptera: five species of Arctiinae and four of Ithomiinae were recorded as pollinators. These male insects are known to obtain alkaloids (through the nectar) in flowers of Asteraceae and Boraginaceae. However, the flowers of E. densiflorum are nectarless, despite presenting a cuniculus (a likely nectariferous cavity). Pollinators insert their proboscides into the flowers and remove or deposit the pollinaria while searching for nectar. The floral tube is very narrow, and insects struggle for up to 75 min to get rid of the flowers. Plants are pollinator-dependent and nearly fully self-incompatible. Pollinarium removal, pollination, and fruiting success (2.85%) were very low; facts that are consistent with the patterns globally observed in deceptive (rewardless) orchids. Nilsson’s male efficiency factor (0.245) was also low, indicating pollen loss in the system. Based on our field observations, we suggest that the fragrance of E. densiflorum likely mimics these plants that are normally used as a source of alkaloids by male Lepidoptera, a hypothesis that we intend to test in the future.
Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops
Authors: Drenker Christian , El Mazouar Doris , Bücker Gerrit , Weißhaupt Sonja , Wienke Eveline , Koch Eckhard , Kunz Stefan , Reineke Annette , Rondot Yvonne , Linkies Ada
Published: February 2023

Although synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environment are urgently needed. In this context, we evaluated eight isolates of the genus Lysobacter (mainly Lysobacter enzymogenes) for their activity against plant pathogens. In vitro, the investigated Lysobacter isolates showed broad antagonistic activity against several phytopathogenic fungi, oomycetes and bacteria. Enzyme assays revealed diverse activities for the tested isolates. The most promising L. enzymogenes isolate (LEC) was used for further detailed analyses of its efficacy and effective working concentrations. The experiments included in vitro spore and sporangia germination tests and leaf disc assays as well as ad planta growth chamber trials against Alternaria solani and Phytophthora infestans on tomato plants, Pseudoperonospora cubensis on cucumbers and Venturia inaequalis on young potted apple trees. When applied on leaves, dilutions of a culture suspension of LEC had a concentration-dependent, protective effect against the tested pathogens. In all pathosystems tested, the effective concentrations were in the range of 2.5–5% and similarly efficacious to common plant protection agents containing copper hydroxide, wettable sulphur or fenhexamid. Thus, the isolate of L. enzymogenes identified in this study exhibits a broad activity against common plant pathogens and is therefore a promising candidate for the development of a microbial biocontrol agent.
Analysis and Mitigation of Pulse-Pile-Up Artifacts in Plasma Pulse-Height X-ray Spectra
Authors: Ahsan Taosif. , Swanson Charles P. S. , Galea Chris , Vinoth Sangeeta P. , Qian Tony , Rubin Tal , Cohen Samuel A.
Published: February 2023

Pulse pile-up in pulse-height energy analyzers increases when the incident rate of pulses increases relative to the inverse of the dead time per pulse of the detection system. Changes in the observed energy distributions with incident rate and detector-electronics-formed pulse shape then occur. We focus on weak high energy tails in X-ray spectra, important for measurements on partially ionized, warm (50–500 eV average electron energy), pure hydrogen plasma. A first-principles two-photon pulse-pile-up model is derived specific to trapezoidal-shaped pulses; quantitative agreement is found between the measurements and the model’s predictions. The model is then used to diagnose pulse-pile-up tail artifacts and mitigate them in relatively low count-rate spectra.
Investigation of Morphological, Chemical, and Thermal Properties of Biodegradable Food Packaging Films Synthesised by Direct Utilisation of Cassava (Monihot esculanta) Bagasse
Authors: Weligama Thuppahige Vindya Thathsaranee , Moghaddam Lalehvash , Welsh Zachary G. , Karim Azharul
Published: February 2023

The utilisation of edible sources of starch such as corn, wheat, potato, and cassava has become the common approach to develop biodegradable food packaging. However, the future food security issue from the wide application of such edible starch sources has become a major concern. Consequently, exploring non-edible sources of starch for starch-based biodegradable food packaging and their property enhancement have become one of the common research interests. Although there has been a great potentials of synthesising biodegradable food packaging by direct utilisation of agro-industrial waste cassava bagasse, there have been very limited studies on this. In this context, the current study investigated the potential of developing biodegradable food packaging by directly using cassava bagasse as an alternative matrix. Two film-forming mixtures were prepared by incorporating glycerol (30% and 35%), powdered cassava bagasse and water. The films were hot-pressed at 60 °C, 100 °C, and 140 °C temperatures under 0.28 t pressure for 6 min. The best film-forming mixture and temperature combination was further tested with 0.42 t and 0.84 t pressures, followed by analysing their morphology, functional group availability and the thermal stability. Accordingly, application of 35% glycerol, with 100 °C, 0.42 t temperature and pressure, respectively, were found to be promising for film preparation. The absence of starch agglomerates in film surfaces with less defects suggested satisfactory dispersion and compatibility of starch granules and glycerol. The film prepared under 0.42 t exhibited slightly higher thermal stability. Synthesised prototypes of food packaging and the obtained characterisation results demonstrated the high feasibility of direct utilisation of cassava bagasse as an alternative, non-edible matrix to synthesise biodegradable food packaging.
A Thermo-Responsive Polymer Micelle with a Liquid Crystalline Core
Authors: Mizoue Yoko , Takahashi Rintaro , Sakurai Kazuo , Yusa Shin-ichi
Published: February 2023

An amphiphilic diblock copolymer (PChM-PNIPAM), composed of poly(cholesteryl 6-methacryloyloxy hexanoate) (PChM) and poly(N-isopropyl acrylamide) (PNIPAM) blocks, was prepared via reversible addition–fragmentation chain transfer radical polymerization. The PChM and PNIPAM blocks exhibited liquid crystalline behavior and a lower critical solution temperature (LCST), respectively. PChM-PNIPAM formed water-soluble polymer micelles in water below the LCST because of hydrophobic interactions of the PChM blocks. The PChM and PNIPAM blocks formed the core and hydrophilic shell of the micelles, respectively. With increasing temperature, the molecular motion of the pendant cholesteryl groups increased, and a liquid crystalline phase transition occurred from an amorphous state in the core. With further increases in temperature, the PNIPAM block in the shell exhibited the LCST and dehydrated. Hydrophobic interactions of the PNIPAM shells resulted in inter-micellar aggregation above the LCST.
Variation in the Optical Properties of PEO-Based Composites via a Green Metal Complex: Macroscopic Measurements to Explain Microscopic Quantum Transport from the Valence Band to the Conduction Band
Authors: Muheddin Daron Q. , Aziz Shujahadeen B. , Mohammed Pshko A.
Published: February 2023

In this study, a green chemistry method was used to synthesize polymer composites based on polyethylene oxide (PEO). The method of the remediation of metal complexes used in this study is an environmentally friendly procedure with a low cost. Zinc metal ion (Zn2+)-polyphenol (PPHNL) complexes were synthesized for two minutes via the combination of a black tea leaf (BTL) extract solution with dissolved Zn-acetate. Then, UV–Vis and FTIR were carried out for the Zn-PPHNL complexes in a liquid and solid. The FTIR spectra show that BTLs contain sufficient functional groups (O-H, C-H, C=O, C=C, C-O, C-N, and N-H), PPHNL, and conjugated double bonds to produce metal complexes by capturing the cations of Zn-acetate salt. Moreover, FTIR of the BTL and Zn–PPHNL complexes approves the formation of the Zn-PPHNL complex over the wide variation in the intensity of bands. The UV absorption spectra of BTL and Zn-PPHNL indicate complex formation among tea PPHNL and Zn cations, which enhances the absorption spectra of the Zn-PPHNL to 0.1 compared to the figure of 0.01 associated with the extracted tea solution. According to an XRD analysis, an amorphous Zn-PPHNL complex was created when Zn2+ ions and PPHNL interacted. Additionally, XRD shows that the structure of the PEO composite becomes a more amorphous structure as the concentration of Zn-PPHNL increases. Furthermore, morphological study via an optical microscope (OM) shows that by increasing the concentration of Zn-PPHNL in a PEO polymer composite the size of the spherulites ascribed to the crystalline phase dramatically decreases. The optical properties of PEO: Zn-PPHNL films, via UV–Vis spectroscopy, were rigorously studied. The Eg is calculated by examining the dielectric loss, which is reduced from 5.5 eV to 0.6 eV by increasing the concentration of Zn-PPHNL in the PEO samples. In addition, Tauc’s form was used to specify the category of electronic transitions in the PEO: Zn-PPHNL films. The impact of crystalline structure and morphology on electronic transition types was discussed. Macroscopic measurable parameters, such as the refractive index and extinction coefficient, were used to determine optical dielectric loss. Fundamental optical dielectric functions were used to determine some key parameters. From the viewpoint of quantum transport, electron transitions were discussed. The merit of this work is that microscopic processes related to electron transition from the VB to the CB can be interpreted interms of measurable macroscopic quantities.
Effective Removal of Glyphosate from Aqueous Systems Using Synthesized PEG-Coated Calcium Peroxide Nanoparticles: Kinetics Study, H2O2 Release Performance and Degradation Pathways
Authors: Li Fan , Choong Thomas Shean Yaw , Abdullah Luqman Chuah , Md. Jamil Siti Nurul Ain , Amerhaider Nuar Nurul Nazihah
Published: February 2023

Glyphosate (N-phosphonomethyl glycine) is a non-selective, broad-spectrum organophosphate herbicide. Its omnipresent application with large quantity has made glyphosate as a problematic contaminant in water. Therefore, an effective technology is urgently required to remove glyphosate and its metabolites from water. In this study, calcium peroxide nanoparticles (nCPs) were functioned as an oxidant to produce sufficient hydroxyl free radicals (·OH) with the presence of Fe2+ as a catalyst using a Fenton-based system. The nCPs with small particle size (40.88 nm) and high surface area (28.09 m2/g) were successfully synthesized via a co-precipitation method. The synthesized nCPs were characterized using transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), Brunauer–Emmett–Teller analysis (BET), dynamic light scattering (DLS), and field emission scanning electron microscopy (FESEM) techniques. Under the given conditions (pH = 3.0, initial nCPs dosage = 0.2 g, Ca2+/Fe2+ molar ratio = 6, the initial glyphosate concentration = 50 mg/L, RT), 99.60% total phosphorus (TP) removal and 75.10% chemical oxygen demand (COD) removal were achieved within 75 min. The degradation process fitted with the Behnajady–Modirshahla–Ghanbery (BMG) kinetics model. The H2O2 release performance and proposed degradation pathways were also reported. The results demonstrated that calcium peroxide nanoparticles are an efficient oxidant for glyphosate removal from aqueous systems.
Sediment Carbon Sequestration and Driving Factors in Seagrass Beds from Hainan Island and the Xisha Islands
Authors: Han Qiuying , Qiu Chongyu , Zeng Wenxuan , Chen Shiquan , Zhao Muqiu , Shi Yunfeng , Zhang Xiaoli
Published: February 2023

Seagrass beds are considered to be substantial sinks of “blue carbon”. However, differentiation in the carbon sink capacities of seagrass beds in different regions with distinct nutrient conditions remains unclear. In this study, sediment carbon stocks, seagrass biomass, and microbial community structures and potential functions of seagrass beds in eutrophic seawater adjacent to Hainan Island and oligotrophic seawater around the Xisha Islands were compared. Our results showed that sediment mineralizable organic carbon and dry bulk density were substantially higher on Hainan Island than on the Xisha Islands (t-test, p < 0.05), while sediment carbon stocks and the total organic carbon were comparable between the two regions (p > 0.05). Similarly, seagrass biomass was much higher on Hainan Island (p < 0.05). Sediment carbon stocks positively correlated with sediment nitrogen and negatively correlated to sediment pH and grain size (p < 0.05). Bacterial diversities were similar in the two regions, while fungi were more diverse on Hainan Island (p < 0.05). Proteobacteria, Desulfobacterota, Ascomycota and Basidiomycota could account for degrading organic carbon on Hainan Island. Proteobacteria and Bacteroidota may contribute primarily to carbon loss in the seagrass beds of the Xisha Islands. This study strengthens our understanding of the effects of human activities on carbon sequestration in seagrass bed ecosystems.
Biotemplating of Al2O3-Doped, CaO-Based Material from Bamboo Fiber for Efficient Solar Energy Storage
Authors: Zhang Haoran , Ma Xiaotong , Huang Xingkang , Li Fei , Li Jia , Hu Xiude , Wang Cuiping
Published: February 2023

The high-temperature sintering of CaO-based materials leads to the serious decay of energy storage performance during the calcination/carbonation cycle. To overcome the loss in porosity problem, an efficient CaO-based material for thermal energy storage was synthesized using bamboo fiber as the biotemplate. The synthesis parameters (bamboo fiber addition, pyrolysis, Al2O3 loading) and the energy storage reaction characteristics of CaO-based energy storage material were optimized on the basis of cyclic calcination/carbonation experiments. The results show that the sacrificed biotemplate enhances the porosity of the synthetic material, denoting improved energy storage density. The cumulative energy storage density of the templated material over 50 cycles is 24,131.44 kJ/kg higher than that of limestone. The carbonation conversion and energy storage density of the templated CaO-based material doped with 5 wt.% Al2O3 and 0.5 g bamboo fiber reach 0.75 mol/mol and 2368.82 kJ/kg after 10 cycles, respectively, which is 2.7 times as high as that of original limestone. The maximum apparent carbonation rate of the templated CaO-based materials in the 1st cycle corresponds to a 240% increment compared to limestone. The maximum calcination rate of the synthetic CaO-based material in the 12th cycle remains 93%, as compared with the initial cycle. The microstructure analysis reveals that the hierarchically-stable structure during the cycle is beneficial for a more effective exposure of surface reactive sites for CaO and inward/outward diffusion for CO2 molecules through CaO. The method using the sacrificed biological template provides an advanced approach to fabricate porous materials, and the composite CaO-based material provides high-return solar energy storage for a potential application in industrial scale.
Perceived Body Image towards Disordered Eating Behaviors and Supplement Use: A Study of Mauritian Gym-Goers
Authors: Moty Numrata , Somanah Jhoti , Putteeraj Manish
Published: February 2023

Purpose: Individuals with body image distortions tend to develop an obsession with calorie intake and weight, leading to compounded coping strategies such as modified eating behaviors, supplement use, and excessive exercise. In the local context, little is known about the equation connecting body image to improper eating behaviors and other potential coping mechanisms. This study primarily aims at critically identifying the nexus between features of disordered eating behavior including supplement use, and body image. Methodology: A probabilistic randomized sample of 305 gym members was assessed based on their level of body satisfaction and its influence on their eating habits and supplement use through a structured survey. Findings: Only 37% of the participants were satisfied with their body image. The results concurred with the notion of a poor body image influencing body esteem (χ2 (10) = 137.21, p < 0.05; Cramer V = 0.474). While exercise motivated the use of supplements, a significant relationship was also observed between disordered eating habits and supplement use (χ2 (4) = 48.63, p < 0.05; Cramer V = 0.399). A predictive model was successfully drawn inclusive of the disordered eating behaviors dimensions as a potent predictor of body image (χ2 (56) = 397.12, p < 0.001. Implications: This study presents an in-depth analysis of the magnitude of disordered eating behaviors with respect to individuals in the local fitness industry, and the need for professional support programs toward the re-alignment of body image in an effort to curtail the development of eating disorders in Mauritian gym-goers.
Electrodermal Activity Implicating a Sympathetic Nervous System Response under the Perception of Sensing a Divine Presence—A Psychophysiological Analysis
Authors: Walter Yoshija , Altorfer Andreas
Published: February 2023

Previous studies have suggested that religious worship experiences may recruit the autonomic nervous system (ANS) in an activating fashion. For this reason, we hypothesized that measurements of the electrodermal activity (EDA) would concur with the notion that the subjective experience of sensing the presence of God recruits a sympathetic nervous system response. We analyzed the EDA of 37 evangelical participants and calculated classic galvanic skin response (GSR) measures. Our experimental design included six conditions with and without music consisting of religious and non-religious songs plus a resting-state condition, which were used to induce a variance in the religious experience suitable for statistical analyses. Results showed that both tonic and phasic signals as well as the overall electrical skin conductance (SC) were positively associated with the religious experience, defined as sensing the presence of God. This implicates that we can accept the hypothesis that such a religious experience under the influence of worship seems to recruit the sympathetic nervous system.
Extension of Scattering Power Decomposition to Dual-Polarization Data for Tropical Forest Monitoring
Authors: Sugimoto Ryu , Nakamura Ryosuke , Tsutsumi Chiaki , Yamaguchi Yoshio
Published: February 2023

A new scattering power decomposition method is developed for accurate tropical forest monitoring that utilizes data in dual-polarization mode instead of quad-polarization (POLSAR) data. This improves the forest classification accuracy and helps to realize rapid deforestation detection because dual-polarization data are more frequently acquired than POLSAR data. The proposed method involves constructing scattering power models for dual-polarization data considering the radar scattering scenario of tropical forests (i.e., ground scattering, volume scattering, and helix scattering). Then, a covariance matrix is created for dual-polarization data and is decomposed to obtain three scattering powers. We evaluated the proposed method by using simulated dual-polarization data for the Amazon, Southeast Asia, and Africa. The proposed method showed an excellent forest classification performance with both user’s accuracy and producer’s accuracy at >98% for window sizes greater than 7 × 14 pixels, regardless of the transmission polarization. It also showed a comparable deforestation detection performance to that obtained by POLSAR data analysis. Moreover, the proposed method showed better classification performance than vegetation indices and was found to be robust regardless of the transmission polarization. When applied to actual dual-polarization data from the Amazon, it provided accurate forest map and deforestation detection. The proposed method will serve tropical forest monitoring very effectively not only for future dual-polarization data but also for accumulated data that have not been fully utilized.
BiTSRS: A Bi-Decoder Transformer Segmentor for High-Spatial-Resolution Remote Sensing Images
Authors: Liu Yuheng , Zhang Yifan , Wang Ye , Mei Shaohui
Published: February 2023

Semantic segmentation of high-spatial-resolution (HSR) remote sensing (RS) images has been extensively studied, and most of the existing methods are based on convolutional neural network (CNN) models. However, the CNN is regarded to have less power in global representation modeling. In the past few years, methods using transformer have attracted increasing attention and generate improved results in semantic segmentation of natural images, owing to their powerful ability in global information acquisition. Nevertheless, these transformer-based methods exhibit limited performance in semantic segmentation of RS images, probably because of the lack of comprehensive understanding in the feature decoding process. In this paper, a novel transformer-based model named the bi-decoder transformer segmentor for remote sensing (BiTSRS) is proposed, aiming at alleviating the problem of flexible feature decoding, through a bi-decoder design for semantic segmentation of RS images. In the proposed BiTSRS, the Swin transformer is adopted as encoder to take both global and local representations into consideration, and a unique design module (ITM) is designed to deal with the limitation of input size for Swin transformer. Furthermore, BiTSRS adopts a bi-decoder structure consisting of a Dilated-Uper decoder and a fully deformable convolutional network (FDCN) module embedded with focal loss, with which it is capable of decoding a wide range of features and local detail deformations. Both ablation experiments and comparison experiments were conducted on three representative RS images datasets. The ablation analysis demonstrates the contributions of specifically designed modules in the proposed BiTSRS to performance improvement. The comparison experimental results illustrate that the proposed BiTSRS clearly outperforms some state-of-the-art semantic segmentation methods.