bluuebunny/crossref_metadata_2025 huggingface dataset:
Lung adenocarcinoma patients with malignant pleural effusions in hot adaptive immunity status have a longer overall survival
Authors: Wu Cheng-Guang , Casanova Ruben , Mairinger Fabian , Soltermann Alex
Published: October 2022

Malignant pleural effusion (MPE) is a common complication of lung adenocarcinoma (LADC) which is associated with a dismal prognosis. We investigated the prognostic role of PD-L1 and other immunomodulators expression in the immune compartment of MPE immune composition. MPE cytologic cell blocks of 83 LADC patients were analysed for the mRNA expression of 770 cancer-immune genes by the NanoString nCounter platform. The expression of relevant immune cell lineage markers was validated by immunohistochemistry (IHC) using quantitative pathology. The mRNA immune profiling identified four MPE patient clusters (C). C1/2 (adaptive+, hot) showed better overall survival (OS) than C3/4 (adaptive-, cold). Additionally, cold immunity profiles (adaptive-), C4 (innate+) were associated with worse OS than C3 (innate-). High PD-L1 expression was linked to the regulation of T cell activation and interferon signalling pathways. Genes of pattern recognition receptor and type I interferon signalling pathways were specifically upregulated in the long-survival (≥90 days) patient group. Moreover, immunomodulators were co-activated and highly expressed in hot adaptive immunity patient clusters, whereas CD274 (PD-L1), TNFRSF9 (4-1BB), VEGFA (VEGF-A) and CD276 (B7-H3) were upregulated in the groups referred as cold. The patient cluster, age and PD-L1 expression were independent prognosticators for LADC MPE patients (p-value < 0.05). Our study sheds light on the variances of immune contexture regarding different PD-L1 expression and survival conditions. It revealed four distinct prognostic patient clusters with specific immune cell components and immunomodulator expression profiles, which, collectively, is supportive for future therapeutic and prognosis for cancer management.
Update in collecting duct carcinoma: Current aspects of the clinical and molecular characterization of an orphan disease
Authors: Suarez Cristina , Marmolejo David , Valdivia Augusto , Morales-Barrera Rafael , Gonzalez Macarena , Mateo Joaquin , Semidey Maria Eugenia , Lorente David , Trilla Enrique , Carles Joan
Published: October 2022

Collecting duct renal cell carcinoma (cdRCC), which until recently was thought to arise from the collecting ducts of Bellini in the renal medulla, is a rare and aggressive type of non-clear renal cell carcinoma (ncRCC), accounting for 1% of all renal tumors and with nearly 50% of patients being diagnosed with Stage IV disease. The median overall survival in this setting is less than 12 months. Several regimens of chemotherapies had been used based on morphologic and cytogenetic similarities with urothelial cell carcinoma described previously, although the prognosis still remains poor. The use of targeted therapies also did not result in favorable outcomes. Recent works using NGS have highlighted genomic alterations in SETD2, CDKN2A, SMARCB1, and NF2. Moreover, transcriptomic studies have confirmed the differences between urothelial carcinoma and cdRCC, the possible true origin of this disease in the distal convoluted tubule (DCT), differentiating from other RCC (e.g., clear cell and papillary) that derive from the proximal convoluted tubule (PCT), and enrichment in immune cells that may harbor insights in novel treatment strategies with immunotherapy and target agents. In this review, we update the current aspects of the clinical, molecular characterization, and new targeted therapeutic options for Collecting duct carcinoma and highlight the future perspectives of treatment in this setting.
Prognostic value of cuproptosis-related genes signature and its impact on the reshaped immune microenvironment of glioma
Authors: Chen Siliang , Zhang Shuxin , Yuan Yunbo , Wang Zhihao , Li Junhong , Li Tengfei , Zuo Mingrong , Feng Wentao , Chen Mina , Liu Yanhui
Published: October 2022

Glioma is the most prevalent malignancy in the central nervous system. The impact of ion-induced cell death on malignant tumors’ development and immune microenvironment has attracted broad attention in recent years. Cuproptosis is a novel copper-dependent mechanism that could potentially regulate tumor cell death by targeting mitochondria respiration. However, the role of cuproptosis in gliomas remains unclear. In the present study, we investigated the relationships between the expression of cuproptosis-related genes (CRGs) and tumor characteristics, including prognosis and microenvironment of glioma, by analyzing multiple public databases and our cohort. Consensus clustering based on the expression of twelve CRGs stratified the glioma patients into three subgroups with significantly different prognosis and immune microenvironment landscapes. Reduced immune infiltration was associated with the less aggressive CRG cluster. A prognostic CRGs risk signature (CRGRS), based on eight critical CRGs, classified the patients into low- and high-risk groups in the training set and was endorsed by validation sets from multiple cohorts. The high-risk group manifested a shorter overall survival, and further survival analysis demonstrated that the CRGRS was an independent prognostic factor. The nomogram combining CRGRS and other clinicopathological factors exhibited good accuracy in predicting the prognosis of glioma patients. Moreover, analyses of tumor immune microenvironment indicated that higher CRGRS was correlated with increased immune cell infiltration but diminished immune function. Gliomas in the high-risk group exhibited higher expression of multiple immune checkpoints, including PD-1 and PD-L1, and a better predicted therapy response to immune checkpoint inhibitors. In conclusion, our study elucidated the connections between CRGs expression and the aggressiveness of gliomas, and the application of CRGRS derived a new robust model for prognosis evaluation of glioma patients. The correlations between the profiles of CRGs expression and immune tumor microenvironment illuminated prospects and potential indications of immunotherapy for glioma.
Daidzein is the in vivo active compound of Puerariae Lobatae Radix water extract for muscarinic receptor-3 inhibition against overactive bladder
Authors: Qiang Yining , Bai Lu , Tian Shuran , Ma Yi , Xu Pingxiang , Cheng Mingchang , Wu Yi , Li Xiaorong , Xue Ming , Zhou Xuelin
Published: October 2022

Background: In the previous study, Puerariae Lobatae Radix (named Gegen in Chinese) water extract attenuated M3 receptor agonist carbachol-induced detrusor contraction after 3-week oral administration in a hypertension-associated OAB (overactive bladder) model. This research aimed to investigate the active ingredients from Gegen water extract against OAB.

Methods: Bioassay-guided fractionation was performed by using preparative HPLC for fast isolation of fractions followed by screening their ex vivo activity through carbachol-induced bladder strip contraction assay. Chemicals in each active fraction were analyzed by HPLC-UV. Urine metabolites were quantified by LC-MS/MS after sub-acute administration. Thermal shift assay with the recombinant human M3 receptor protein was performed, and molecular docking analysis was used for molecular modelling of M3 receptor inhibition.

Results: Bioassay-guided fractionation results for isolating M3 receptor inhibitors indicated that four compounds were identified as active ingredients of Gegen water extract, and their inhibition potency on carbachol-induced detrusor contraction was ranked in descending order according to their inhibition concentrations as follows: genistein > daidzein > biochanin A >> puerarin. Daidzein in urine reached an ex vivo effective concentration to inhibit detrusor contraction, but others did not. Daidzein concentration-dependently increased the melt temperature (Tm) of recombinant human M3 receptor protein with a positive binding (ΔTm = 2.12 °C at 100 μg/ml). Molecular docking analysis showed that daidzein can potently bind to the ligand binding pocket of the M3 receptor via hydrogen bonding.

Conclusion: Puerarin and its derivatives were pro-drugs, and daidzein was their in vivo active form via M3 receptor inhibition for treating OAB.
Fasting plasma glucose and HbA1c levels predict the risk of type 2 diabetes and diabetic retinopathy in a Thai high-risk population with prediabetes
Authors: Washirasaksiri Chaiwat , Srivanichakorn Weerachai , Borrisut Nutsakol , Sitasuwan Tullaya , Tinmanee Rungsima , Kositamongkol Chayanis , Ariyakunaphan Pinyapat , Auesomwang Chonticha , Sayabovorn Naruemit , Chaisathaphol Thanet , Phisalprapa Pochamana
Published: October 2022

Introduction: The incidences of diabetes and diabetic retinopathy (DR) in Thai high-risk individuals with prediabetes have not been identified. This study compared diabetes and DR incidences among people at risk with different glycemic levels, using fasting plasma glucose (FPG) and hemoglobin A1C (HbA1c).

Materials and methods: A historical cohort study estimating risk of type 2 diabetes and DR was conducted among outpatients, using FPG and HbA1c measurements at recruitment and monitored for ≥5 years. High-risk participants (defined as having metabolic syndrome or atherosclerotic cardiovascular disease) were categorized by glycemic level into 4 groups: 1) impaired fasting glucose (IFG)-/HbA1c- (FPG <110 mg/dl; HbA1c < 6.0%); 2) IFG+/HbA1c- (FPG 110–125 mg/dl; HbA1c < 6.0%); 3) IFG-/HbA1c+ (FPG <110 mg/dl; HbA1c 6.0%–6.4%); and 4) IFG+/HbA1c+ (FPG 110–125 mg/dl; HbA1c 6.0%–6.4%). The incidences of type 2 diabetes mellitus (T2DM) and DR were obtained and estimated using Kaplan-Meier analysis. Cox regression models explored hazard ratios (HRs).

Results: We recruited 8,977 people at risk (metabolic syndrome, 89.9%; atherosclerotic cardiovascular disease, 16.9%). The baseline cohort consisted of 1) IFG-/HbA1c- (n = 4,221; 47.0%); 2) IFG+/HbA1c- (n = 1,274; 14.2%); 3) IFG-/HbA1c+ (n = 2,151; 24.0%); and 4) IFG+/HbA1c+ (n = 1,331; 14.8%). Their 5-year T2DM incidences were 16.0%, 26.4%, 30.8%, and 48.5% (p < 0.001). The median DR follow-up was 7.8 years (interquartile range, 7.0–8.4 years). The DR incidences were 0.50, 0.63, 1.44, and 2.68/1,000 person-years (p < 0.001) for IFG-/HbA1c-, IFG+/HbA1c-, IFG-/HbA1c+, and IFG+/HbA1c+, respectively. Compared with IFG-/HbA1c-, the multivariable-adjusted HRs (95% CI) for incident diabetes were 1.94 (1.34–2.80), 2.45 (1.83–3.29), and 4.56 (3.39–6.15) for IFG+/HbA1c-, IFG-/HbA1c+, and IFG+/HbA1c+, respectively. As for incident DR, the corresponding HRs were 0.67 (0.08–5.76), 4.74 (1.69–13.31), and 5.46 (1.82–16.39), respectively.

Conclusion: The 5-year incidence of T2DM in Thai high-risk participants with prediabetes was very high. The incidences of diabetes and DR significantly increased with higher degrees of dysglycemia. High-risk people with FPG 110–125 mg/dl and HbA1c 6.0%–6.4% were more likely to develop T2DM and DR. Such individuals should receive priority lifestyle and pharmacological management.
Formulation of ciprofloxacin-loaded oral self-emulsifying drug delivery system to improve the pharmacokinetics and antibacterial activity
Authors: Asghar Asghar Ali , Akhlaq Muhammad , Jalil Aamir , Azad Abul Kalam , Asghar Junaid , Adeel Muhammad , Albadrani Ghadeer M. , Al-Doaiss Amin A. , Kamel Mohamed , Altyar Ahmed E. , Abdel-Daim Mohamed M.
Published: October 2022

This study aims to increase the aqueous solubility of ciprofloxacin (CPN) to improve oral bioavailability. This was carried out by formulating a stable formulation of the Self-Emulsifying Drug Delivery System (SEDDS) using various ratios of lipid/oil, surfactant, and co-surfactant. A pseudo-ternary phase diagram was designed to find an area of emulsification. Eight formulations (F1-CPN–F8-CPN) containing oleic acid oil, silicone oil, olive oil, castor oil, sunflower oil, myglol oil, polysorbate-80, polysorbate-20, PEO-200, PEO-400, PEO-600, and PG were formulated. The resultant SEDDS were subjected to thermodynamic study, size, and surface charge studies to improve preparation. Improved composition of SEDDS F5-CPN containing 40% oil, 60% polysorbate-80, and propylene glycol (Smix ratio 6: 1) were thermodynamically stable emulsions having droplet size 202.6 nm, charge surface -13.9 mV, and 0.226 polydispersity index (PDI). Fourier transform infra-red (FT-IR) studies revealed that the optimized formulation and drug showed no interactions. Scanning electron microscope tests showed the droplets have an even surface and spherical shape. It was observed that within 5 h, the concentration of released CPN from optimized formulations F5-CPN was 93%. F5-CPN also showed a higher antibacterial action against S. aurous than free CPN. It shows that F5-CPN is a better formulation with a good release and high antibacterial activity.
Role of CD40(L)-TRAF signaling in inflammation and resolution—a double-edged sword
Authors: Strohm Lea , Ubbens Henning , Münzel Thomas , Daiber Andreas , Daub Steffen
Published: October 2022

Cardiovascular diseases (CVD) and cardiovascular risk factors are the leading cause of death in the world today. According to the Global Burden of Disease Study, hypertension together with ischemic heart and cerebrovascular diseases is responsible for approximately 40% of all deaths worldwide. The major pathomechanism underlying almost all CVD is atherosclerosis, an inflammatory disorder of the vascular system. Recent large-scale clinical trials demonstrated that inflammation itself is an independent cardiovascular risk factor. Specific anti-inflammatory therapy could decrease cardiovascular mortality in patients with atherosclerosis (increased markers of inflammation). Inflammation, however, can also be beneficial by conferring so-called resolution, a process that contributes to clearing damaged tissue from cell debris upon cell death and thereby represents an essential step for recovery from, e.g., ischemia/reperfusion damage. Based on these considerations, the present review highlights features of the detrimental inflammatory reactions as well as of the beneficial process of immune cell-triggered resolution. In this context, we discuss the polarization of macrophages to either M1 or M2 phenotype and critically assess the role of the CD40L-CD40-TRAF signaling cascade in atherosclerosis and its potential link to resolution. As CD40L can bind to different cellular receptors, it can initiate a broad range of inflammatory processes that may be detrimental or beneficial. Likewise, the signaling of CD40L downstream of CD40 is mainly determined by activation of TRAF1-6 pathways that again can be detrimental or beneficial. Accordingly, CD40(L)-based therapies may be Janus-faced and require sophisticated fine-tuning in order to promote cardioprotection.
Metabolism of Paeoniae Radix Rubra and its 14 constituents in mice
Authors: Zhang Jing , Lv Yang , Zhang Jing , Shi Wen-Jin , Guo Xu-Yan , Xu Jing-Jing , Wang Peng-Pu , Chen Xue-Tai , Xiang Lin-Han , Xu Feng , Wang Xuan , Cai Shao-Qing
Published: October 2022

Objective: Paeoniae Radix Rubra (PRR) is a commonly used traditional Chinese medicine with the effects of clearing away heat, cooling the blood, and relieving blood stasis. To 1) elucidate the metabolites and metabolic pathways of PRR and its 14 main constituents in mice and 2) reveal the possible origins of the known effective forms of PRR and their isomers, the metabolism of PRR in mice was systematically studied for the first time.

Methods: PRR and its 14 constituents were administered to mice by gavage once a day for seven consecutive days, respectively. All urine and feces were collected during the 7 days of dosing, and blood was collected at 1 h after the last dose. Metabolites were detected and identified using high performance liquid chromatography with diode array detector and combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry (HPLC-DAD-ESI-IT-TOF-MSn).

Results: In total, 23, 16, 24, 17, 18, 30, 27, 17, 22, 17, 33, 3, 8, 24, and 31 metabolites of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin, hydroxybenzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, lactiflorin, epicatechin gallate, catechin gallate, catechin, ellagic acid, 3,3′-di-O-methylellagic acid, methylgallate, and PRR were respectively identified in mice; after eliminating identical metabolites, a total of 195 metabolites remained, including 8, 11, 25, 17, 18, 30, 27, 17, 21, 17, 1, 2, 8, 20, and 20 newly identified metabolites, respectively. The metabolic reactions of PRR and its 14 main constituents in mice were primarily methylation, hydrogenation, hydrolysis, hydroxylation, glucuronidation, and sulfation.

Conclusion: We elucidated the metabolites and metabolic pathways of PRR and its 14 constituents (e.g., paeoniflorin, catechin, ellagic acid, and gallic acid) in mice and revealed the possible origins of the 10 known effective forms of PRR and their isomers. The findings are of great significance to studying the mechanism of action and quality control of PRR.
E-models of inflation and primordial black holes
Authors: Frolovsky Daniel , Ketov Sergei V. , Saburov Sultan
Published: October 2022

We propose and study the new (generalized) E-type α-attractor models of inflation, in order to include formation of primordial black holes (PBHs). The inflaton potential has a near-inflection point where slow-roll conditions are violated, thus leading to large scalar perturbations collapsing to PBHs later. An ultra-slow roll (short) phase exists between two (longer) phases of slow-roll inflation. We numerically investigate the phases of inflation, derive the power spectrum of scalar perturbations and calculate the PBHs masses. For certain values of the parameters, the asteroid-size PBHs can be formed with the masses of 1017 ÷ 1019 g, beyond the Hawking evaporation limit and in agreement with current Cosmic Microwave Background observations. Those PBHs are a candidate for (part of) dark matter in the present Universe, while the gravitational waves induced by the PBHs formation may be detectable by the future space-based gravitational interferometers.
Finite element simulations of inclined magnetic field and mixed convection in an enclosure with periodically heated walls in the presence of an obstacle
Authors: Khan Y. , Mahmood Rashid , Majeed Afraz Hussain , Irshad Sadia , Alameer A. , Faraz N.
Published: October 2022

The current manifestation is utilized to explicate the inspiration of combined convection flows in a cavity with a square cylinder placed at the center of the cavity having coordinates (0.5, 0.5). To be more specific, right- and left-sided vertical walls are kept cold, and the lower wall is to be heated in uniform and non-uniform manners, while the upper horizontal wall is moved with a constant velocity ULid and is thermally adiabatic. The obstacle is treated as cold as well as thermally adiabatic, and the no-slip velocity boundary conditions are specified at its surface. For the purpose of computing the velocity profile and temperature, a space including the quadratic polynomials (P2) is chosen; however, the pressure has been approximated using a linear ( P1 ) finite element space of functions. The Newton technique is used to perform the computations needed to solve the discrete systems of non-linear algebraic equations. The non-linear iterations are terminated at residual below 10–6, whereas the inner core linear solver is based on Gaussian elimination with special reordering of unknowns. To show the consistency of the implemented numerical technique, the parametric study is designed based on the most relevant non-dimensional parameters, namely, the Grashof number Gr ranging from 102 to 105, the Hartmann number Ha changing from 0 to 50, and the Reynolds number Re varying from 10 to 200. Computations in the forms of velocity streamlines and isotherm contour profiles are adorned. In addition, the production of temperature disparities is associated with an increase in Nuavg as Re increases. In contrast, a diminishing aptitude in kinetic energy is observed due to the creation of Lorentz forces.
A robot-driven automatic scribing method via three-dimensional measurement sensor
Authors: Luo Hua , Zhang Ke , Li Ruifeng , Liang Peidong , Li Zhongwei
Published: October 2022

The evaluation of the complex workpiece allowance and the scribing the datum line now extremely rely on manual operation. The manual operation is time-consuming and prone to misjudgment, and has a large blind area. To solve these problems, the robot-driven automatic scribing via three-dimensional (3D) measurement sensor method (RAS3DM) is proposed. In this study, we solve the key technology of calibrating the rigid transformation between the 3D measurement coordinate system and laser scribing coordinate system. And a fast and accurate multiple coordinate systems calibration and unification method is proposed, a fixed calibration target and a mobile calibration target are designed. Hence, a structured-light 3D shape measurement sensor driven by an industrial robot with six-degree of freedom (6-DOF) is utilized to obtain the complete 3D shape of the complex workpiece automatically, and then by comparing the 3D measurement result and the standard CAD model, the laser scribing equipment can automatically scribe the mechanical processing datum line to assist further processing. The experiment results illustrate that the proposed method is accurate and efficient: the complex workpiece allowance evaluation and scribing time is about 5 times faster than that of manual operation, and the scribing accuracy is 0.15mm, which can effectively solve various problems of manual scribing. The proposed RAS3DM can be widely applied in factories and laboratories.
Progressive resistance training for children with cerebral palsy: A randomized controlled trial evaluating the effects on muscle strength and morphology
Authors: Hanssen Britta , Peeters Nicky , De Beukelaer Nathalie , Vannerom Astrid , Peeters Leen , Molenaers Guy , Van Campenhout Anja , Deschepper Ellen , Van den Broeck Christine , Desloovere Kaat
Published: October 2022

Children with spastic cerebral palsy often present with muscle weakness, resulting from neural impairments and muscular alterations. While progressive resistance training (PRT) improves muscle weakness, the effects on muscle morphology remain inconclusive. This investigation evaluated the effects of a PRT program on lower limb muscle strength, morphology and gross motor function. Forty-nine children with spastic cerebral palsy were randomized by minimization. The intervention group (nparticipants = 26, age: 8.3 ± 2.0 years, Gross Motor Function Classification System [GMFCS] level I/II/III: 17/5/4, nlegs = 41) received a 12-week PRT program, consisting of 3-4 sessions per week, with exercises performed in 3 sets of 10 repetitions, aiming at 60%–80% of the 1-repetition maximum. Training sessions were performed under supervision with the physiotherapist and at home. The control group (nparticipants = 22, age: 8.5 ± 2.1 year, GMFCS level I/II/III: 14/5/3, nlegs = 36) continued usual care including regular physiotherapy and use of orthotics. We assessed pre- and post-training knee extension, knee flexion and plantar flexion isometric strength, rectus femoris, semitendinosus and medial gastrocnemius muscle morphology, as well as functional strength, gross motor function and walking capacity. Data processing was performed blinded. Linear mixed models were applied to evaluate the difference in evolution over time between the control and intervention group (interaction-effect) and within each group (time-effect). The α-level was set atp= 0.01. Knee flexion strength and unilateral heel raises showed a significant interaction-effect (p≤ 0.008), with improvements in the intervention group (p≤ 0.001). Moreover, significant time-effects were seen for knee extension and plantar flexion isometric strength, rectus femoris and medial gastrocnemius MV, sit-to-stand and lateral step-up in the intervention group (p≤ 0.004). Echo-intensity, muscle lengths and gross motor function showed limited to no changes. PRT improved strength and MV in the intervention group, whereby strength parameters significantly or close to significantly differed from the control group. Although, relative improvements in strength were larger than improvements in MV, important effects were seen on the maintenance of muscle size relative to skeletal growth. In conclusion, this study proved the effectiveness of a home-based, physiotherapy supervised, PRT program to improve isometric and functional muscle strength in children with SCP without negative effects on muscle properties or any serious adverse events.

Clinical Trial Registration:ClinicalTrials.gov, identifier NCT03863197.
Isometric skeletal muscle contractile properties in common strains of male laboratory mice
Authors: Minchew Everett C. , Williamson Nicholas C. , Readyoff Andrew T. , McClung Joseph M. , Spangenburg Espen E.
Published: October 2022

Assessing contractile function of skeletal muscle in murine models is a commonly employed laboratory technique that investigators utilize to measure the impact of genetic manipulations, drug efficacy, or other therapeutic interventions. Often overlooked is the potential for the strain of the mouse to influence the functional properties of the skeletal muscle. Thus, we sought to characterize commonly assessed isometric force measures in the hindlimb muscles across a variety of mouse strains. Using 6-8-week-old male mice, we measured isometric force, fatigue susceptibility, relaxation kinetics, muscle mass, myofiber cross-sectional area, and fiber type composition of the extensor digitorum longus (EDL) and soleus muscles in C57BL/6NJ, BALB/cJ, FVB/NJ, C57BL/6J, and C57BL/10 mice. The data demonstrate both unique differences and a number of similarities between both muscles in the various genetic backgrounds of mice. Soleus muscle specific force (i.e., force per unit size) exhibited higher variation across strains while specific force of the EDL muscle exhibited minimal variation. In contrast, absolute force differed only in a few mouse strains whereas analysis of muscle morphology revealed many distinctions when compared across all the groups. Collectively, the data suggest that the strain of the mouse can potentially influence the measured biological outcome and may possibly promote a synergistic effect with any genetic manipulation or therapeutic intervention. Thus, it is critical for the investigator to carefully consider the genetic background of the mouse used in the experimental design and precisely document the strain of mouse employed during publication.
Effect of exercise training on nitric oxide and nitrate/nitrite (NOx) production: A systematic review and meta-analysis
Authors: Arefirad Tahereh , Seif Ehsan , Sepidarkish Mahdi , Mohammadian Khonsari Nami , Mousavifar Seyedeh Azam , Yazdani Shahrooz , Rahimi Fatemeh , Einollahi Faezeh , Heshmati Javad , Qorbani Mostafa
Published: October 2022

Background:Exercise and physical activity can improve circulation through various mechanisms, such as the increment of nitric oxide (NO) production, by affecting vascular endothelial nitric oxide synthase, and reducing reactive oxygen species (ROS). Although, theoretically, this mechanism is well known, studies in living subjects have made controversial findings regarding the association of NO production and its metabolites [nitrate/nitrite (NOx)] with physical activity. Hence, this systematic review and meta-analysis was designed to gather all these studies and evaluate the effects of exercise training, and physical activity duration and length on the mean change of serum/plasma NO and NOx.

Method:We searched all available bibliographic electronic databases from inception through to May 2022 to include all randomized controlled trials (RCT) and quasi-experimental trials which assessed the effect of exercise and training on NO and NOx levels. Random-effects meta-analysis was used to pool the standardized mean difference (SMD) and 95% confidence interval (CI) of included RCT studies which assessed the effect of training. Stratified meta-analysis was performed according to the type of exercise (high-intensity interval training (HIIT), aerobic training (AT), the duration of exercise (≤8 and > 8 weeks), and length of exercise in each session ≥40 and 40 < minutes).

Results:Overall, 15 and 10 studies were included in the systematic review and meta-analysis, respectively. According to the random-effects meta-analysis, exercise significantly increased the mean change of NO and NOx compared to control (SMD: 1.82, 95%CI: 1.14 to 2.49. In the stratified meta-analysis, the mean change of NO and NOx in the intervention group was significantly higher than in the control group in the AT (SMD: 1.36, 95%CI: 0.55–2.18), HIIT (SMD: 2.55, 95%CI: 1.14–3.96), duration of ≤8 (SMD: 2.29, 95%CI: 1.24–3.35) and > 8 weeks (SMD: 1.19, 95%CI: 0.52–1.86), length of ≥40 (SMD: 1.61, 95%CI: 1.04–2.18), and 40 < minutes in each session (SMD: 2.07, 95%CI: 0.79–3.35).

Conclusion:The findings of this study indicate that, regardless of exercise duration, length, and type (AT or HIIT), exercise can significantly increase serum NO and NOx levels.
Prediction approach of larch wood density from visible–near-infrared spectroscopy based on parameter calibrating and transfer learning
Authors: Zhang Zheyu , Li Yaoxiang , Li Ying
Published: October 2022

Wood density, as a key indicator to measure wood properties, is of weighty significance in enhancing wood utilization and modifying wood properties in sustainable forest management. Visible–near-infrared (Vis-NIR) spectroscopy provides a feasible and efficient solution for obtaining wood density by the advantages of its efficiency and non-destructiveness. However, the spectral responses are different in wood products with different moisture content conditions, and changes in external factors may cause the regression model to fail. Although some calibration transfer methods and convolutional neural network (CNN)-based deep transfer learning methods have been proposed, the generalization ability and prediction accuracy of the models still need to be improved. For the prediction problem of Vis-NIR wood density in different moisture contents, a deep transfer learning hybrid method with automatic calibration capability (Resnet1D-SVR-TrAdaBoost.R2) was proposed in this study. The disadvantage of overfitting was avoided when CNN processes small sample data, which considered the complex exterior factors in actual production to enhance feature extraction and migration between samples. Density prediction of the method was performed on a larch dataset with different moisture content conditions, and the hybrid method was found to achieve the best prediction results under the calibration samples with different target domain calibration samples and moisture contents, and the performance of models was better than that of the traditional calibration transfer and migration learning methods. In particular, the hybrid model has achieved an improvement of about 0.1 in both R2 and root mean square error (RMSE) values compared to the support vector regression model transferred by piecewise direct standardization method (SVR+PDS), which has the best performance among traditional calibration methods. To further ascertain the generalizability of the hybrid model, the model was validated with samples collected from mixed moisture contents as the target domain. Various experiments demonstrated that the Resnet1D-SVR-TrAdaBoost.R2 model could predict larch wood density with a high generalization ability and accuracy effectively but was computation consuming. It showed the potential to be extended to predict other metrics of wood.
Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
Authors: Aher Rasika Rajendra , Reddy Palakolanu Sudhakar , Bhunia Rupam Kumar , Flyckt Kayla S. , Shankhapal Aishwarya R. , Ojha Rabishankar , Everard John D. , Wayne Laura L. , Ruddy Brian M. , Deonovic Benjamin , Gupta Shashi K. , Sharma Kiran K. , Bhatnagar-Mathur Pooja
Published: October 2022

Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular mechanisms of rancidity development in the flour from contrasting inbred lines under accelerated aging conditions. The breakdown of storage lipids (triacylglycerols; TAG) was accompanied by free fatty acid accumulation over the time course for all lines. The high rancidity lines had the highest amount of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity. Additionally, the high rancidity lines manifested substantial amounts of volatile aldehyde compounds, which are characteristic products of lipid oxidation. Lipases with expression in seed post-milling were sequenced from low and high rancidity lines. Polymorphisms were identified in two TAG lipase genes (PgTAGLip1 and PgTAGLip2) from the low rancidity line. Expression in a yeast model system confirmed these mutants were non-functional. We provide a direct mechanism to alleviate rancidity in pearl millet flour by identifying mutations in key TAG lipase genes that are associated with low rancidity. These genetic variations can be exploited through molecular breeding or precision genome technologies to develop elite pearl millet cultivars with improved flour shelf life.
Comparative transcriptome analysis of Citrus macrophylla tree infected with Citrus tristeza virus stem pitting mutants provides new insight into the role of phloem regeneration in stem pitting disease
Authors: Khalilzadeh Maryam , Weber Kyle Clark , Dutt Manjul , El-Mohtar Choaa Amine , Levy Amit
Published: October 2022

Stem pitting is a complex and economically important virus-associated disease of perennial woody plants. Molecular mechanisms and pathways occurring during virus-plant interaction that result in this phenomenon are still obscure. Previous studies indicated that different Citrus tristeza virus (CTV) mutants induce defined stem pitting phenotypes ranging from mild (CTVΔp13) to severe (CTVΔp33) in Citrus macrophylla trees. In this study, we conducted comparative transcriptome analyses of C. macrophylla trees infected with CTV mutants (CTVΔp13 and CTVΔp33) and a full-length virus in comparison to healthy plants as control. The mild CTV stem pitting mutant had very few differentially expressed genes (DEGs) related to plant defense mechanism and plant growth and development. In contrast, substantial gene expression changes were observed in plants infected with the severe mutant and the full-length virus, indicating that both the p13 and p33 proteins of CTV acted as a regulator of symptom production by activating and modulating plant responses, respectively. The analysis of transcriptome data for CTVΔp33 and the full-length virus suggested that xylem specification has been blocked by detecting several genes encoding xylem, cell wall and lignin degradation, and cell wall loosening enzymes. Furthermore, stem pitting was accompanied by downregulation of transcription factors involved in regulation of xylem differentiation and downregulation of some genes involved in lignin biosynthesis, showing that the xylem differentiation and specification program has been shut off. Upregulation of genes encoding transcription factors associated with phloem and cambium development indicated the activation of this program in stem pitting disease. Furthermore, we detected the induction of several DEGs encoding proteins associated with cell cycle re-entry such as chromatin remodeling factors and cyclin, and histone modification. This kind of expression pattern of genes related to xylem differentiation and specification, phloem and cambium development, and cell cycle re-entry is demonstrated during secondary vascular tissue (SVT) regeneration. The microscopy analysis confirmed that the regeneration of new phloem is associated with stem pitting phenotypes. The findings of this study, thus, provide evidence for the association between stem pitting phenotypes and SVT regeneration, suggesting that the expression of these genes might play important roles in development of stem pitting symptoms. Overall, our findings suggest that phloem regeneration contributes to development of stem pitting symptoms.
Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis
Authors: Wan Dongli , Wan Yongqing , Zhang Tongrui , Wang Ruigang , Ding Yong
Published: October 2022

Heavy grazing significantly reduces Stipa grandis growth. To enhance our understanding of plant responses to heavy grazing, we conducted transcriptomic, proteomic, and metabolic analyses of the leaves of non-grazed plants (NG) and heavy-grazing-induced dwarf plants (HG) of S. grandis. A total of 101 metabolites, 167 proteins, and 1,268 genes differed in abundance between the HG and NG groups. Analysis of Kyoto Encyclopedia of Genes and Genomes pathways among differentially accumulated metabolites (DAMs) revealed that the most enriched pathways were flavone and flavonol biosynthesis, tryptophan metabolism, and phenylpropanoid biosynthesis. An integrative analysis of differentially expressed genes (DEGs) and proteins, and DAMs in these three pathways was performed. Heavy-grazing-induced dwarfism decreased the accumulation of DAMs enriched in phenylpropanoid biosynthesis, among which four DAMs were associated with lignin biosynthesis. In contrast, all DAMs enriched in flavone and flavonol biosynthesis and tryptophan metabolism showed increased accumulation in HG compared with NG plants. Among the DAMs enriched in tryptophan metabolism, three were involved in tryptophan-dependent IAA biosynthesis. Some of the DEGs and proteins enriched in these pathways showed different expression trends. The results indicated that these pathways play important roles in the regulation of growth and grazing-associated stress adaptions of S. grandis. This study enriches the knowledge of the mechanism of heavy-grazing-induced growth inhibition of S. grandis and provides valuable information for restoration of the productivity in degraded grassland.
Prenatal allostatic load and preterm birth: A systematic review
Authors: Premji Shahirose Sadrudin , Pana Gianella Santos , Cuncannon Alexander , Ronksley Paul E. , Dosani Aliyah , Hayden K. Alix , Lalani Sharifa , Musana Joseph Wangira , Shaikh Kiran , Yim Ilona S. ,   Maternal-infant Global Health Team (MiGHT) Collaborators in Research
Published: October 2022

Objective

Allostatic load refers to cumulative neuroendocrine burden and has been postulated to mediate and moderate physiological and psychological stress-related responses. This may have important implications for the risk of preterm birth. This systematic review examines the evidence on the association between prenatal allostatic load and preterm birth.

Data sources

A comprehensive search of seven electronic databases was conducted from inception to August 23, 2022 to identify all English-language observational and mixed methods studies examining allostatic load and preterm birth with no year or geographic restrictions.

Study eligibility criteria

Studies were included if they measured allostatic load, evaluated as the cumulative effect of any combination of more than one allostatic load biomarker, during pregnancy. Studies must have observed preterm birth, defined as < 37 weeks' gestational age, as a primary or secondary outcome of interest.

Study appraisal and synthesis methods

The Quality In Prognosis Studies tool was used to evaluate risk of bias within included studies. A narrative synthesis was conducted to explore potential associations between allostatic load and preterm birth, and sources of heterogeneity.

Results

Three prospective cohort studies were identified and revealed mixed evidence for an association between allostatic load and preterm birth. One study reported a statistically significant association while the other two studies reported little to no evidence for an association. Heterogeneity in when and how allostatic load was measured, limitations in study design and cohort socio-demographics may have contributed to the mixed evidence.

Conclusions

This review provides insight into key individual-, community-, and study-level characteristics that may influence the association between allostatic load and preterm birth. Knowledge gaps are identified as foci for future research, including heterogeneity in allostatic load biomarkers and allostatic load index algorithms as well as pregnancy-specific considerations for allostatic load measurement. Further investigation of the allostatic load framework in the context of perinatal mental health is needed to advance understandings of maternal, infant, and child health.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020208990, PROSPERO, identifier: CRD42020208990.
Authoritarian leadership and cyberloafing: A moderated mediation model of emotional exhaustion and power distance orientation
Authors: Zhang Yajun , Wang Jingjing , Akhtar Muhammad Naseer , Wang Yongqi
Published: October 2022

Drawing upon the conservation of resource theory (COR), this study proposes a moderated mediation model of authoritarian leadership on subordinates’ cyberloafing. Paired samples of 360 employees working in 103 teams from Chinese companies were collected at 2 points in time. The results show that authoritarian leadership positively affects subordinates’ cyberloafing and this relationship is mediated by emotional exhaustion. Furthermore, power distance orientation negatively moderates the relationship between authoritarian leadership and emotional exhaustion and also negatively moderates the indirect effect of authoritarian leadership on subordinates’ cyberloafing through subordinates’ emotional exhaustion. Our findings expand and enrich the literature on authoritarian leadership and cyberloafing and have significant practical implications for managing employees in this digital era.
RETRACTED: The approaches and methods of music psychology in the relationship between music emotion and cognition in music teaching activities
Authors: Jia Nan , Yang Chongze
Published: October 2022

In order to further improve the effect of music teaching, more music psychology should be applied in music teaching to assist teaching, and students should better understand the emotional elements reflected in music through music emotion and cognitive teaching. This essay starts from the relationship between music emotion and cognition, to deeply explore the application of music psychology in teaching activities, through the construction of music education psychological regulation function model to explore the effect of the application of psychology in music teaching. The results showed that the scores of positive emotions were significantly improved, while the scores of negative emotions were significantly decreased. The difference between the improvement and reduction of positive emotions was significant (p < 0.01, p < 0.01). The results show that psychology based on the relationship between emotions and people is helpful to improve the effectiveness of music teaching. And on this basis put forward the music teaching activity innovation path.
A test of indirect grounding of abstract concepts using multimodal distributional semantics
Authors: Utsumi Akira
Published: October 2022

How are abstract concepts grounded in perceptual experiences for shaping human conceptual knowledge? Recent studies on abstract concepts emphasizing the role of language have argued that abstract concepts are grounded indirectly in perceptual experiences and language (or words) functions as a bridge between abstract concepts and perceptual experiences. However, this “indirect grounding” view remains largely speculative and has hardly been supported directly by empirical evidence. In this paper, therefore, we test the indirect grounding view by means of multimodal distributional semantics, in which the meaning of a word (i.e., a concept) is represented as the combination of textual and visual vectors. The newly devised multimodal distributional semantic model incorporates the indirect grounding view by computing the visual vector of an abstract word through the visual vectors of concrete words semantically related to that abstract word. An evaluation experiment is conducted in which conceptual representation is predicted from multimodal vectors using a multilayer feed-forward neural network. The analysis of prediction performance demonstrates that the indirect grounding model achieves significantly better performance in predicting human conceptual representation of abstract words than other models that mimic competing views on abstract concepts, especially than the direct grounding model in which the visual vectors of abstract words are computed directly from the images of abstract concepts. This result lends some plausibility to the indirect grounding view as a cognitive mechanism of grounding abstract concepts.
Comparison of the acute effects of Tai chi versus high-intensity interval training on inhibitory control in individuals with substance use disorder
Authors: Yin Yanqiang , Yang Suyong , Xiao Ke , Wang Tianyuan , Wang Jiabin , Schöllhorn Wolfgang I. , Zhu Dong , Pang Xiaowu
Published: October 2022

Objective

The purpose of this study is to investigate the effects of a single session of Tai chi (TC) exercise and high-intensity interval training (HIIT) on inhibitory control in individuals with substance use disorder (SUD).

Methods

A total 47 males with methamphetamine dependence were recruited from a compulsory drug rehabilitation center; participation in this study was voluntary. The participants were randomly assigned to the TC group or the HIIT group, and computer-based Go/No-go and Stroop tasks were used to assess inhibitory control in an indoor setting prior to and following exercise. Independent samplet-test was applied for baseline comparison of continuous variables, while analysis of variance was applied to test differences in the effect of each intervention before and after a single session of exercise.

Results

In Go/No-go test, the reaction time of the TC and HIIT groups in the post-test was shorter than that at the baseline, and the response accuracy of the post-test were higher than that of the baseline. In the Stroop task, the reaction time of two groups in the post-test was shorter than that at the baseline; while, greater improvement in response accuracy was observed in HIIT group in the post-test than that of the baseline.

Conclusion

Both TC and HIIT can promote inhibitory control in individuals with SUD. Compared with the TC group, the HIIT group showed greater improvements in response accuracy. These findings demonstrate the potential of TC and HIIT in improving cognition in SUD.

Clinical trial registration

[http://www.chictr.org.cn/], identifier [ChiCTR1900022158].
Developmental assets and positive youth development in Brazilian university students
Authors: de Jesus Maurício Coelho , Dutra-Thomé Luciana , Pereira Anderson Siqueira
Published: October 2022

The Positive Youth Development (PYD) describes an intersection between young people and their context, emphasizing characteristics of a healthy development. The PYD’s 5Cs occur when there is an alignment between healthy individual characteristics and contextual resources. This study investigated the PYD’s 5Cs associations with the perception of social connections (family, community, academic), mental health, and stressful events. The sample was composed of 495 Brazilian College students aged between 18 and 33 years, who answered a survey with 59 questions about reflexive, constructive, and healthy behaviors. Descriptive, correlational, and regression analysis through structural equation modeling were conducted. The results focused on the role of family, community and educational institution in the PYD promotion. These results highlight the relevant contributions of social support in the construction of protective strategies of coping with stressful events and in the promotion of health behaviors and well-being, particularly in the university context.