DILUTED AND DYNAMIZED SOLUTION OF Euphorbia tirucalli: REVISITING. LIPID TROPISM IN CHRONIC TOXICOLOGY?
Authors: Gomes Nelson Bretas de Noronha , Nagamatsu Denise , Bolognani Fábio de Almeida , de Freitas Francisco José , de Freitas Janete Moura Vieira , Varricchio Marcia Cristina Braga Nunes , Pyrrho Alexandre dos Santos , Branco Morgana Teixeira Lima Castelo 
Published: December 2024

Total extract of Euphorbia tirucalli (Aveloz – stem with latex) is used in ethnomedicine and by several Traditional Communities to induce tumor regression orally. However, it has toxic effects. At the clinical level, after authorization from the Research Ethics Committee at the Hahnemannian Institute of Brazil, from 1997 to 2010, complementary treatment with homeopathic medicines applicable to the reduction of clinical manifestations of the Cancer State was aimed at the potential reduction of inflammatory endothelial injury assessed by measuring serum C-Reactive Protein (CRP). Difficult-to-control Systemic Arterial Hypertension, major depression, obesity secondary to binge eating and eating errors, and plurimetabolic syndrome are associated with elevated serum TNF-α and CRP levels, markers of local and systemic endothelial damage. They are therefore proposed as biomarkers for monitoring the pharmacological action of ultradiluted and dynamized solutions authorized by the National Health Surveillance Agency. They are also used as a strategy in psychosocial care to promote mental health through a good sleep and wakefulness pattern. Constant oxidative stress activates protein kinase C isoforms in the cell membrane, participating as a triggering factor for prevalent clinical diseases in a society that demands lifestyle changes and social vulnerabilities. The present study was designed to evaluate the effects of high dilutions of the species collected in Seropedica/RJ/Brazil in healthy female mice for a period of 18 weeks. The ultradiluted and dynamized solution (UDS) of the 70% total ethanolic extract of aerial parts of E. tirucalli was obtained through the interaction of two processes: 1:100 dilution by weight and succussion, using 30% ethanol as solvent until the dilution of 30CH (SUD 30CH), purchased monetarily from the Professor José de Barros School Pharmacy of the Hahnemannian Institute of Brazil, only for this assay authorized by the Research Ethics Committee/UFRJ. The control solutions were composed (ethanolic HD) in the same dilutions and 10 drops diluted in drinking water were administered simultaneously orally ad libitum for chronic toxicology. SUD 30CH of the Mother Tincture (TM) of E. tirucalli did not compromise the life or development of the mice over 18 weeks (water consumption, food, weight; p > 0.05). There was no significant variation in liver and spleen weight, nor in serum leukocytes, but it suggested peripheral recruitment, which is the ability to increase the first barrier of nonspecific defense. It was not nephrotoxic, but it was hyperglycemic and slightly hepatotoxic. It caused specific damage to serum fats, possibly by lipid peroxidation, reducing LDL, triglycerides and phospholipids. Histopathological analysis showed that it was not toxic to lymphocytes in the splenic parenchyma. It suggested an increase in the shape and activity of germinal centers (organized and hyperstained) in the presence of a molar concentration equivalent to 10-60. Furthermore, the secretion of the cytokine Tumor Necrosis Factor-α (TNF-α) at serum level remained normal (p> 0.05), suggesting the absence of immunosuppressive action, an observation contrary to that described in the literature for the ponderal use of the ethanolic extract. TNF-α is a cytokine secreted by macrophages and neurons, among other cell types. Its reduction in serum levels has already been correlated with major depression, and has been investigated in other types of depression. Qualitatively, it was observed that there was a change in the behavior of healthy female Swiss mice challenged with the test solution. There was a reduction in fear of daily handling by the handler, an increase in exploratory behavior of the environment and attention. The test group was the one that had the most difficulty waking up in the morning, and curiously they began to sleep in a row and under the light (instead of nestled in the dark at the bottom of the plastic box). In our society, sleep disorders are growing at a dizzying rate, triggering Systemic Arterial Hypertension, Obesity, Depression, Fibromyalgia, among other conditions that can be included in the Cancer State. Furthermore, the difference in laboratory results for that chronic toxicology test for SUD 30CH obtained from latex trituration corroborates the theory that the active-inactive interaction should be taken into account to ensure biological responses induced by ultradiluted and dynamized solutions. Aiming to deepen the understanding of the potential mechanism of action as a “phytoadaptogen-like”, defined by the FDA for neurobehavioral tests, it is proposed to investigate SUD 30CH obtained from total aqueous extract of E. tirucalli from Seropedica, Euphorbium officinarum and Euphorbia resinifera associated with diluted but non-dynamized controls (hormetic, excluding nanoeffect) and dynamized solvent controls, in order to also investigate not only oxygen, but also sulfur and nitrogen way of redox potential.