bluuebunny/crossref_metadata_2025 huggingface dataset:
The Interaction between microRNAs and the Wnt/β-Catenin Signaling Pathway in Osteoarthritis
Authors: Shang Xiaobin , Böker Kai Oliver , Taheri Shahed , Hawellek Thelonius , Lehmann Wolfgang , Schilling Arndt F.
Published: September 2021

Osteoarthritis (OA) is a chronic disease affecting the whole joint, which still lacks a disease-modifying treatment. This suggests an incomplete understanding of underlying molecular mechanisms. The Wnt/β-catenin pathway is involved in different pathophysiological processes of OA. Interestingly, both excessive stimulation and suppression of this pathway can contribute to the pathogenesis of OA. microRNAs have been shown to regulate different cellular processes in different diseases, including the metabolic activity of chondrocytes and osteocytes. To bridge these findings, here we attempt to give a conclusive overview of microRNA regulation of the Wnt/β-catenin pathway in bone and cartilage, which may provide insights to advance the development of miRNA-based therapeutics for OA treatment.
Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
Authors: Perincherry Lakshmipriya , Urbaniak Monika , Pawłowicz Izabela , Kotowska Karolina , Waśkiewicz Agnieszka , Stępień Łukasz
Published: September 2021

Fusarium species are common plant pathogens that cause several important diseases. They produce a wide range of secondary metabolites, among which mycotoxins and extracellular cell wall-degrading enzymes (CWDEs) contribute to weakening and invading the host plant successfully. Two species of Fusarium isolated from peas were monitored for their expression profile of three cell wall-degrading enzyme coding genes upon culturing with extracts from resistant (Sokolik) and susceptible (Santana) pea cultivars. The extracts from Santana induced a sudden increase in the gene expression, whereas Sokolik elicited a reduced expression. The coherent observation was that the biochemical profile of the host plant plays a major role in regulating the fungal gene expression. In order to uncover the fungal characteristics in planta, both pea cultivars were infected with two strains each of F. proliferatum and F. oxysporum on the 30th day of growth. The enzyme activity assays from both roots and rhizosphere indicated that more enzymes were used for degrading the cell wall of the resistant host compared to the susceptible host. The most commonly produced enzymes were cellulase, β-glucosidase, xylanase, pectinase and lipase, where the pathogen selectively degraded the components of both the primary and secondary cell walls. The levels of beauvericin accumulated in the infected roots of both cultivars were also monitored. There was a difference between the levels of beauvericin accumulated in both the cultivars, where the susceptible cultivar had more beauvericin than the resistant one, showing that the plants susceptible to the pathogen were also susceptible to the toxin accumulation.
A Novel L-Asparaginase from Hyperthermophilic Archaeon Thermococcus sibiricus: Heterologous Expression and Characterization for Biotechnology Application
Authors: Dumina Maria , Zhgun Alexander , Pokrovskaya Marina , Aleksandrova Svetlana , Zhdanov Dmitry , Sokolov Nikolay , El’darov Michael
Published: September 2021

L-asparaginase (L-ASNase) is a vital enzyme with a broad range of applications in medicine and food industry. Drawbacks of current commercial L-ASNases stimulate the search for better-producing sources of the enzyme, and extremophiles are especially attractive in this view. In this study, a novel L-asparaginase originating from the hyperthermophilic archaeon Thermococcus sibiricus (TsA) was expressed in Escherichia coli, purified and characterized. The enzyme is optimally active at 90 °C and pH 9.0 with a specific activity of 2164 U/mg towards L-asparagine. Kinetic parameters KM and Vmax for the enzyme are 2.8 mM and 1200 µM/min, respectively. TsA is stable in urea solutions 0–6 M and displays no significant changes of the activity in the presence of metal ions Ni2+, Cu2+, Mg2+, Zn2+ and Ca2+ and EDTA added in concentrations 1 and 10 mmol/L except for Fe3+. The enzyme retains 86% of its initial activity after 20 min incubation at 90 °C, which should be enough to reduce acrylamide formation in foods processed at elevated temperatures. TsA displays strong cytotoxic activity toward cancer cell lines K562, A549 and Sk-Br-3, while normal human fibroblasts WI-38 are almost unsensitive to it. The enzyme seems to be a promising candidate for further investigation and biotechnology application.
Wood Formation under Severe Drought Invokes Adjustment of the Hormonal and Transcriptional Landscape in Poplar
Authors: Yu Dade , Janz Dennis , Zienkiewicz Krzysztof , Herrfurth Cornelia , Feussner Ivo , Chen Shaoliang , Polle Andrea
Published: September 2021

Drought is a severe environmental stress that exerts negative effects on plant growth. In trees, drought leads to reduced secondary growth and altered wood anatomy. The mechanisms underlying wood stress adaptation are not well understood. Here, we investigated the physiological, anatomical, hormonal, and transcriptional responses of poplar to strong drought. Drought-stressed xylem was characterized by higher vessel frequencies, smaller vessel lumina, and thicker secondary fiber cell walls. These changes were accompanied by strong increases in abscisic acid (ABA) and antagonistic changes in salicylic acid in wood. Transcriptional evidence supported ABA biosynthesis and signaling in wood. Since ABA signaling activates the fiber-thickening factor NST1, we expected upregulation of the secondary cell wall (SCW) cascade under stress. By contrast, transcription factors and biosynthesis genes for SCW formation were down-regulated, whereas a small set of cellulose synthase-like genes and a huge array of genes involved in cell wall modification were up-regulated in drought-stressed wood. Therefore, we suggest that ABA signaling monitors normal SCW biosynthesis and that drought causes a switch from normal to “stress wood” formation recruiting a dedicated set of genes for cell wall biosynthesis and remodeling. This proposition implies that drought-induced changes in cell wall properties underlie regulatory mechanisms distinct from those of normal wood.
The Intra- and Extra-Telomeric Role of TRF2 in the DNA Damage Response
Authors: Imran Siti A. M. , Yazid Muhammad Dain , Cui Wei , Lokanathan Yogeswaran
Published: September 2021

Telomere repeat binding factor 2 (TRF2) has a well-known function at the telomeres, which acts to protect the telomere end from being recognized as a DNA break or from unwanted recombination. This protection mechanism prevents DNA instability from mutation and subsequent severe diseases caused by the changes in DNA, such as cancer. Since TRF2 actively inhibits the DNA damage response factors from recognizing the telomere end as a DNA break, many more studies have also shown its interactions outside of the telomeres. However, very little has been discovered on the mechanisms involved in these interactions. This review aims to discuss the known function of TRF2 and its interaction with the DNA damage response (DDR) factors at both telomeric and non-telomeric regions. In this review, we will summarize recent progress and findings on the interactions between TRF2 and DDR factors at telomeres and outside of telomeres.
RFCM-PALM: In-Silico Prediction of S-Palmitoylation Sites in the Synaptic Proteins for Male/Female Mouse Data
Authors: Bandyopadhyay Soumyendu Sekhar , Halder Anup Kumar , Zaręba-Kozioł Monika , Bartkowiak-Kaczmarek Anna , Dutta Aviinandaan , Chatterjee Piyali , Nasipuri Mita , Wójtowicz Tomasz , Wlodarczyk Jakub , Basu Subhadip
Published: September 2021

S-palmitoylation is a reversible covalent post-translational modification of cysteine thiol side chain by palmitic acid. S-palmitoylation plays a critical role in a variety of biological processes and is engaged in several human diseases. Therefore, identifying specific sites of this modification is crucial for understanding their functional consequences in physiology and pathology. We present a random forest (RF) classifier-based consensus strategy (RFCM-PALM) for predicting the palmitoylated cysteine sites on synaptic proteins from male/female mouse data. To design the prediction model, we have introduced a heuristic strategy for selection of the optimum set of physicochemical features from the AAIndex dataset using (a) K-Best (KB) features, (b) genetic algorithm (GA), and (c) a union (UN) of KB and GA based features. Furthermore, decisions from best-trained models of the KB, GA, and UN-based classifiers are combined by designing a three-star quality consensus strategy to further refine and enhance the scores of the individual models. The experiment is carried out on three categorized synaptic protein datasets of a male mouse, female mouse, and combined (male + female), whereas in each group, weighted data is used as training, and knock-out is used as the hold-out set for performance evaluation and comparison. RFCM-PALM shows ~80% area under curve (AUC) score in all three categories of datasets and achieve 10% average accuracy (male—15%, female—15%, and combined—7%) improvements on the hold-out set compared to the state-of-the-art approaches. To summarize, our method with efficient feature selection and novel consensus strategy shows significant performance gains in the prediction of S-palmitoylation sites in mouse datasets.
Multi-Attribute Group Decision-Making Based on Interval-Valued q-Rung Orthopair Fuzzy Power Generalized Maclaurin Symmetric Mean Operator and Its Application in Online Education Platform Performance Evaluation
Authors: Wang Jun , Zhou Yang
Published: September 2021

This paper aims to propose a novel multi-attribute group decision-making (MAGDM) method based on interval-valued q-rung orthopair fuzzy sets (IVq-ROFSs). The IVq-ROFSs have been proved to be effective in handling MAGDM problems, and several novel decision-making methods have been proposed. Nevertheless, it is worth pointing out that these approaches still have some limitations, and there still exist some realistic situations that cannot be solved by existing MAGDM methods. Hence, the objective of this paper is to introduce a novel MAGDM method, which can overcome some of the drawbacks of existing approaches. To effectively and appropriately aggregate interval-valued q-rung orthopair fuzzy numbers (IVq-ROFNs), we combine the power average with generalized Maclaurin symmetric mean (GMSM), propose the power GMSM operator and extend it into IVq-ROFSs. Afterwards, a collection of new aggregation operators for IVq-ROFNs are developed. In this paper, we study definitions of these operators and investigate their characteristics as well as special cases. Then, based on the new aggregation operators, we present a new MAGDM method. Finally, we apply the proposed MAGDM method in online education platform performance evaluation to illustrate its effectiveness and validity. In addition, we also provide comparative analysis to explain why decision-makers should use our method instead of the others.
Following Pediatric and Adult IBD Patients through the COVID-19 Pandemic: Changes in Psychosocial Burden and Perception of Infection Risk and Harm over Time
Authors: Koletzko Leandra , Klucker Elisabeth , Le Thi Thu Giang , Breiteneicher Simone , Rubio-Acero Raquel , Neuhaus Lukas , Stark Reneé G. , Standl Marie , Wieser Andreas , Török Helga , Koletzko Sibylle , Schwerd Tobias
Published: September 2021

Background: COVID-19-associated restrictions impact societies. We investigated the impact in a large cohort of inflammatory bowel disease (IBD) patients. Methods: Pediatric (pIBD) and adult patients and pIBD parents completed validated questionnaires for self-perceived stress (Perceived Stress Questionnaire, PSQ) and quality of life from July to October 2020 (1st survey) and March to April 2021 (2nd survey). Analyses were stratified by age groups (6–20, >20–40, >40–60, >60 years). Perceived risk of infection and harm from COVID-19 were rated on a 1–7 scale. An index for severe outcome (SIRSCO) was calculated. Multivariable logistic regression analysis was performed. Results: Of 820 invited patients, 504 (62%, 6–85 years) patients and 86 pIBD parents completed the 1st, thereof 403 (80.4%) the 2nd survey. COVID-19 restrictions resulted in cancelled doctoral appointments (26.7%), decreased physical activity, increased food intake, unintended weight gain and sleep disturbance. PSQ increased with disease activity. Elderly males rated lower compared to females or younger adults. PSQ in pIBD mothers were comparable to moderate/severe IBD adults. Infection risk and harm were perceived high in 36% and 75.4%. Multivariable logistic models revealed associations of higher perceived risk with >3 household members, job conditions and female gender, and of perceived harm with higher SIRSCO, unintended weight change, but not with gender or age. Cancelled clinic-visits were associated with both. SARS-CoV-2 antibodies prior 2nd infection wave were positive in 2/472 (0.4%). Conclusions: IBD patients report a high degree of stress and self-perceived risk of complications from COVID-19 with major differences related to gender and age. Low seroprevalence may indicate altered immune response.
Differences in the Prevalence of Clinical Adjacent Segment Pathology among Continents after Anterior Cervical Fusion: Meta-Analysis of Randomized Controlled Trials
Authors: Chung Young-Woo , Kim Sung-Kyu , Park Yong-Jin
Published: September 2021

Development of adjacent segment pathology leading to secondary operation is a matter of concern after anterior cervical discectomy and fusion (ACDF). Some studies have reported anatomic difference between races, but no epidemiological data on prevalence of clinical adjacent segment pathology (cASP) among races or continents has been published. The purpose of this study was to compare the prevalence of cASP that underwent surgery after monosegmental ACDF among continents by meta-analysis. MEDLINE, EMBASE, and Cochrane Library with manual searching in key journals, reference lists, and the National Technical Information Service were searched from inception to December 2018. Twenty studies with a total of 2009 patients were included in the meta-analysis. We extracted the publication details, sample size, and prevalence of cASP that underwent surgery. A total of 15 papers from North America, three from Europe, and two from Asia met the inclusion criteria. A total number of 2009 patients underwent monosegmental ACDF, and 113 patients (5.62%) among them had cASP that underwent surgery. The rate of cASP that underwent surgery was 4.99% in the North America, 3.65% in the Europe, 6.34% in the Asia, and there were no statistically significant differences (p = 0.63). The current study using the method of meta-analysis revealed that there were no significant differences in the rate of cASP that underwent surgery after ACDF among the continents.
Neural Correlates of Aberrant Salience and Source Monitoring in Schizophrenia and At-Risk Mental States—A Systematic Review of fMRI Studies
Authors: Kowalski Joachim , Aleksandrowicz Adrianna , Dąbkowska Małgorzata , Gawęda Łukasz
Published: September 2021

Cognitive biases are an important factor contributing to the development and symptom severity of psychosis. Despite the fact that various cognitive biases are contributing to psychosis, they are rarely investigated together. In the current systematic review, we aimed at investigating specific and shared functional neural correlates of two important cognitive biases: aberrant salience and source monitoring. We conducted a systematic search of fMRI studies of said cognitive biases. Eight studies on aberrant salience and eleven studies on source monitoring were included in the review. We critically discussed behavioural and neuroimaging findings concerning cognitive biases. Various brain regions are associated with aberrant salience and source monitoring in individuals with schizophrenia and the risk of psychosis. The ventral striatum and insula contribute to aberrant salience. The medial prefrontal cortex, superior and middle temporal gyrus contribute to source monitoring. The anterior cingulate cortex and hippocampus contribute to both cognitive biases, constituting a neural overlap. Our review indicates that aberrant salience and source monitoring may share neural mechanisms, suggesting their joint role in producing disrupted external attributions of perceptual and cognitive experiences, thus elucidating their role in positive symptoms of psychosis. Account bridging mechanisms of these two biases is discussed. Further studies are warranted.
Can Lateralization of Reverse Shoulder Arthroplasty Improve Active External Rotation in Patients with Preoperative Fatty Infiltration of the Infraspinatus and Teres Minor?
Authors: Nabergoj Marko , Onishi Shinzo , Lädermann Alexandre , Kalache Houssam , Trebše Rihard , Bothorel Hugo , Collin Philippe
Published: September 2021

(1) Background: Postoperative recovery of external rotation after reverse shoulder arthroplasty (RSA) has been reported despite nonfunctional external rotator muscles. Thus, this study aimed to clinically determine the ideal prosthetic design allowing external rotation recovery in such a cohort. (2) Methods: A monocentric comparative study was retrospectively performed on patients who had primary RSA between June 2013 and February 2018 with a significant preoperative fatty infiltration of the infraspinatus and teres minor. Two groups were formed with patients with a lateral humerus/lateral glenoid 145° onlay RSA—the onlay group (OG), and a medial humerus/lateral glenoid 155° inlay RSA—the inlay group (IG). Patients were matched 1:1 by age, gender, indication, preoperative range of motion (ROM), and Constant score. The ROM and Constant scores were assessed preoperatively and at a minimum follow-up of two years. (3) Results: Forty-seven patients have been included (23 in OG and 24 in IG). Postoperative external rotation increased significantly in the OG only (p = 0.049), and its postoperative value was significantly greater than that of the IG by 11.1° (p = 0.028). (4) Conclusion: The use of a lateralized humeral stem with a low neck-shaft angle resulted in significantly improved external rotation compared to a medialized humeral 155° stem, even in cases of severe fatty infiltration of the infraspinatus and teres minor. Humeral lateralization and a low neck-shaft angle should be favored when planning an RSA in a patient without a functional posterior rotator cuff.
Acute Coronary Syndrome in the Older Patient
Authors: García-Blas Sergio , Cordero Alberto , Diez-Villanueva Pablo , Martinez-Avial Maria , Ayesta Ana , Ariza-Solé Albert , Mateus-Porta Gemma , Martínez-Sellés Manuel , Escribano David , Gabaldon-Perez Ana , Bodi Vicente , Bonanad Clara
Published: September 2021

Coronary artery disease is one of the leading causes of morbidity and mortality, and its prevalence increases with age. The growing number of older patients and their differential characteristics make its management a challenge in clinical practice. The aim of this review is to summarize the state-of-the-art in diagnosis and treatment of acute coronary syndromes in this subgroup of patients. This comprises peculiarities of ST-segment elevation myocardial infarction (STEMI) management, updated evidence of non-STEMI therapeutic strategies, individualization of antiplatelet treatment (weighting ischemic and hemorrhagic risks), as well as assessment of geriatric conditions and ethical issues in decision making.
Optimized Doppler Estimation and Symbol Synchronization for Mobile M-ary Spread Spectrum Underwater Acoustic Communication
Authors: Yang Guang , Zhou Feng , Qiao Gang , Zhao Yunjiang , Liu Yufei , Lu Yinheng , He Yuanan
Published: September 2021

In mobile underwater acoustic (UWA) communications, the Doppler effect causes severe signal distortion, which leads to carrier frequency shift and compresses/broadens the signal length. This situation has a more severe impact on communication performance in the case of low signal-to-noise ratio and variable-speed movement. This paper proposes a non-data-aided Doppler estimation method for M-ary spread spectrum UWA communication systems in mobile scenarios. The receiver uses the spread spectrum codes dedicated to transmitting signals with different frequency offsets as local reference signals. Correlation operations are performed symbol by symbol with the received signal. The decoding and Doppler estimation of the present symbol are achieved by searching the correlation maximum in the code domain and frequency domain. The length of the current symbol is corrected for the next symbol synchronization using the estimated Doppler coefficient. To optimize the process of Doppler estimation and symbol synchronization, a heuristic search method is used. By adjusting the Doppler factor search step size, setting the threshold value, and using the Doppler factor estimation of the previous symbol, the search range can be significantly reduced and the computational complexity decreased. The Fisher-Yates shuffle algorithm is used to traverse the search range to ensure reliability of the results. Simulation results show that enlarging the frequency-domain search step size in some degree does not affect the decoding accuracy. On 15 May 2021, a shallow-water mobile UWA spread spectrum communication experiment was conducted in Weihai, China. The horizontal distance between the transmitter and the receiver is 3.7–4.0 km, and the communication rate is 41.96 bits per second. The transmitting ship moves at a speed of 0–3 m/s, and the bit error rate (BER) is lower than 1e−3, which is better than that of the sliding correlation despreading method with average Doppler compensation.
Deletion of a Rare Fungal PKS CgPKS11 Promotes Chaetoglobosin A Biosynthesis, Yet Defers the Growth and Development of Chaetomium globosum
Authors: Xiang Biyun , Hao Xiaoran , Xie Qiaohong , Shen Guangya , Liu Yanjie , Zhu Xudong
Published: September 2021

We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the biocontrol of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR-Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase encoding genes, Cgpks11, which is putatively involved in the biosynthesis of chaetoglocin A, was disrupted. Cgpks11 deletion led to the overexpression of the CgcheA gene cluster, which is responsible for ChA biosynthesis, and a 1.6-fold increase of ChA. Transcription of pks-1, a melanin PKS, was simultaneously upregulated. Conversely, the transcription of genes for chaetoglocin A biosynthesis, e.g., CHGG_10646 and CHGG_10649, were significantly downregulated. The deletion also led to growth retardation and seriously impaired ascospore development. This study found a novel regulatory means on the biosynthesis of ChA by CgPKS11. CgPKS11 affects chaetoglobosin A biosynthesis, growth, and development in Chaetomium globosum.
Biocatalytic Syntheses of Antiplatelet Metabolites of the Thienopyridines Clopidogrel and Prasugrel Using Fungal Peroxygenases
Authors: Kiebist Jan , Schmidtke Kai-Uwe , Schramm Marina , König Rosalie , Quint Stephan , Kohlmann Johannes , Zuhse Ralf , Ullrich René , Hofrichter Martin , Scheibner Katrin
Published: September 2021

Antithrombotic thienopyridines, such as clopidogrel and prasugrel, are prodrugs that undergo a metabolic two-step bioactivation for their pharmacological efficacy. In the first step, a thiolactone is formed, which is then converted by cytochrome P450-dependent oxidation via sulfenic acids to the active thiol metabolites. These metabolites are the active compounds that inhibit the platelet P2Y12 receptor and thereby prevent atherothrombotic events. Thus far, described biocatalytic and chemical synthesis approaches to obtain active thienopyridine metabolites are rather complex and suffer from low yields. In the present study, several unspecific peroxygenases (UPOs, EC 1.11.2.1) known to efficiently mimic P450 reactions in vitro—but requiring only hydroperoxide as oxidant—were tested for biocatalytic one-pot syntheses. In the course of the reaction optimization, various parameters such as pH and reductant, as well as organic solvent and amount were varied. The best results for the conversion of 1 mM thienopyridine were achieved using 2 U mL−1 of a UPO from agaric fungus Marasmius rotula (MroUPO) in a phosphate-buffered system (pH 7) containing 5 mM ascorbate, 2 mM h−1 H2O2 and 20% acetone. The preparation of the active metabolite of clopidogrel was successful via a two-step oxidation with an overall yield of 25%. In the case of prasugrel, a cascade of porcine liver esterase (PLE) and MroUPO was applied, resulting in a yield of 44%. The two metabolites were isolated with high purity, and their structures were confirmed by MS and MS2 spectrometry as well as NMR spectroscopy. The findings broaden the scope of UPO applications again and demonstrate that they can be effectively used for the selective synthesis of metabolites and late-state diversification of organic molecules, circumventing complex multistage chemical syntheses and providing sufficient material for structural elucidation, reference material, or cellular assays.
Cytokine and Chemokine Responses in Invasive Aspergillosis Following Hematopoietic Stem Cell Transplantation: Past Evidence for Future Therapy of Aspergillosis
Authors: Thammasit Patcharin , Sripetchwandee Jirapas , Nosanchuk Joshua D. , Chattipakorn Siriporn C. , Chattipakorn Nipon , Youngchim Sirida
Published: September 2021

Invasive pulmonary aspergillosis is a frequent complication in immunocompromised individuals, and it continues to be an important cause of mortality in patients undergoing hematopoietic stem cell transplantation. In addition to antifungal therapy used for mycoses, immune-modulatory molecules such as cytokines and chemokines can modify the host immune response and exhibit a promising form of antimicrobial therapeutics to combat invasive fungal diseases. Cytokine and chemokine profiles may also be applied as biomarkers during fungal infections and clinical research has demonstrated different activation patterns of cytokines in invasive mycoses such as aspergillosis. In this review, we summarize different aspects of cytokines that have been described to date and provide possible future directions in research on invasive pulmonary aspergillosis following hematopoietic stem cell transplantation. These findings suggest that cytokines and chemokines may serve as useful biomarkers to improve diagnosis and monitoring of infection.
Progression Risk Score Estimation Based on Immunostaining Data in Oral Cancer Using Unsupervised Hierarchical Clustering Analysis: A Retrospective Study in Taiwan
Authors: Wang Hui-Ching , Chan Leong-Perng , Wu Chun-Chieh , Hsiao Hui-Hua , Liu Yi-Chang , Cho Shih-Feng , Du Jeng-Shiun , Liu Ta-Chih , Yang Cheng-Hong , Pan Mei-Ren , Moi Sin-Hua
Published: September 2021

This study aimed to investigate whether the progression risk score (PRS) developed from cytoplasmic immunohistochemistry (IHC) biomarkers is available and applicable for assessing risk and prognosis in oral cancer patients. Participants in this retrospective case-control study were diagnosed between 2012 and 2014 and subsequently underwent surgical intervention. The specimens from surgery were stained by IHC for 16 cytoplasmic target markers. We evaluated the results of IHC staining, clinical and pathological features, progression-free survival (PFS), and overall survival (OS) of 102 oral cancer patients using a novel estimation approach with unsupervised hierarchical clustering analysis. Patients were stratified into high-risk (52) and low-risk (50) groups, according to their PRS; a metric consisting of cytoplasmic PLK1, PhosphoMet, SGK2, and SHC1 expression. Moreover, PRS could be extended for use in the Cox proportional hazard regression model to estimate survival outcomes with associated clinical parameters. Our study findings revealed that the high-risk patients had a significantly increased risk in cancer progression compared with low-risk patients (hazard ratio (HR) = 2.20, 95% confidence interval (CI) = 1.10–2.42, p = 0.026). After considering the influences of demographics, risk behaviors, and tumor characteristics, risk estimation with PRS provided distinct PFS groups for patients with oral cancer (p = 0.017, p = 0.019, and p = 0.020). Our findings support that PRS could serve as an ideal biomarker for clinical use in risk stratification and progression assessment in oral cancer.
Effect of Dexmedetomidine on Biochemical Recurrence in Patients after Robot-Assisted Laparoscopic Radical Prostatectomy: A Retrospective Study
Authors: Yoo Young Chul , Jang Won Sik , Kim Ki Jun , Hong Jung Hwa , Yang Sunmo , Kim Na Young
Published: September 2021

The usage of dexmedetomidine during cancer surgery in current clinical practice is debatable, largely owing to the differing reports of its efficacy based on cancer type. This study aimed to investigate the effects of dexmedetomidine on biochemical recurrence (BCR) and radiographic progression in patients with prostate cancer, who have undergone robot-assisted laparoscopic radical prostatectomy (RALP). Using follow-up data from two prospective randomized controlled studies, BCR and radiographic progression were compared between individuals who received dexmedetomidine (n = 58) and those who received saline (n = 56). Patients with complete follow-up records between July 2013 and June 2019 were enrolled in this study. There were no significant between-group differences in the number of patients who developed BCR and those who showed positive radiographic progression. Based on the Cox regression analysis, age (p = 0.015), Gleason score ≥ 8 (p < 0.001), and pathological tumor stage 3a and 3b (both p < 0.001) were shown to be significant predictors of post-RALP BCR. However, there was no impact on the dexmedetomidine or control groups. Low-dose administration of dexmedetomidine at a rate of 0.3–0.4 μg/kg/h did not significantly affect BCR incidence following RALP. In addition, no beneficial effect was noted on radiographic progression.
Topsoil Seed Bank as Feeding Ground for Farmland Birds: A Comparative Assessment in Agricultural Habitats
Authors: Voudouri Aikaterini , Chaideftou Evgenia , Sfougaris Athanassios
Published: September 2021

The topsoil seed bank was studied in four types of agricultural bird habitats: fields with cereals, maize, clover and tilled fields of a Mediterranean plain to determine the potentially richest habitat based on food supply for the wintering farmland birds. The diversity and abundance of topsoil seeds differed between seasons but did not differ significantly between habitats. The cereal habitat was the richest in food supply for the overwintering of farmland birds. The topsoil seed bank was dominated by Chenopodium album, Polygonum aviculare and Amaranthus retroflexus. The findings of this study provide insight for low-intensity management of higher-elevation mount agricultural areas of southern Mediterranean by preserving seed-rich habitats for farmland avifauna.
Study of Triticum aestivum Resistome in Response to Wheat dwarf India Virus Infection
Authors: Kumar Jitendra , Rai Krishan Mohan , Kianian Shahryar F. , Singh Sudhir P.
Published: September 2021

Susceptible and resistant germplasm respond differently to pathogenic attack, including virus infections. We compared the transcriptome changes between a resistant wheat cultivar, Sonalika, and a susceptible cultivar, WL711, to understand this process in wheat against wheat dwarf India virus (WDIV) infection. A total of 2760 and 1853 genes were differentially expressed in virus-infected and mock-inoculated Sonalika, respectively, compared to WL711. The overrepresentation of genes involved in signaling, hormone metabolism, enzymes, secondary metabolites, proteolysis, and transcription factors was documented, including the overexpression of multiple PR proteins. We hypothesize that the virus resistance in Sonalika is likely due to strong intracellular surveillance via the action of multiple PR proteins (PR1, RAR1, and RPM1) and ChiB. Other genes such as PIP1, LIP1, DnaJ, defensins, oxalate oxidase, ankyrin repeat protein, serine-threonine kinase, SR proteins, beta-1,3-glucanases, and O-methyltransferases had a significant differential expression and play roles in stress tolerance, may also be contributing towards the virus resistance in Sonalika. In addition, we identified putative genes with unknown functions, which are only expressed in response to WDIV infection in Sonalika. The role of these genes could be further validated and utilized in engineering resistance in wheat and other crops.
FDG-PET/CT and Auricular Cartilage Biopsy Are Useful for Diagnosing with Relapsing Polychondritis in Patients without Auricular Symptoms
Authors: Okuda Saki , Hirooka Yasuaki , Itami Tetsu , Nozaki Yuji , Sugiyama Masafumi , Kinoshita Koji , Funauchi Masanori , Matsumura Itaru
Published: September 2021

Relapsing polychondritis (RP) is a rare autoimmune inflammatory disease characterized by recurrent inflammation and destruction of cartilage. Although auricular chondritis is a characteristic finding in RP, it can be difficult to diagnose in the absence of auricular symptoms. A 64-year-old Japanese male was referred to our hospital with fever and respiratory distress. Contrast-enhanced computed tomography (CT) revealed bronchial wall thickening and we suspected RP; however, he had no auricular symptoms and did not meet the diagnostic McAdam criteria for RP, so we used 18F-fluorodeoxyglucose positron emission tomography/CT (FDG-PET/CT) to search for other cartilage lesions. This analysis revealed FDG accumulation not only in the bronchial walls, but also in the left auricle. Instead of a bronchial biopsy using a bronchoscope, we performed a biopsy of the left auricular cartilage, which is considered a relatively less invasive site. Even though the auricle was asymptomatic, the pathology results revealed chondritis. He was diagnosed with RP, and his symptoms rapidly improved with corticosteroid therapy. A biopsy of asymptomatic auricular cartilage may be useful in the diagnosis of RP. FDG-PET/CT is a powerful tool for the early diagnosis of RP, identifying inflammatory areas even in the absence of symptoms, and guiding the selection of appropriate biopsy sites.
Structural Insights into Protein Regulation by Phosphorylation and Substrate Recognition of Protein Kinases/Phosphatases
Authors: Seok Seung-Hyeon
Published: September 2021

Protein phosphorylation is one of the most widely observed and important post-translational modification (PTM) processes. Protein phosphorylation is regulated by protein kinases, each of which covalently attaches a phosphate group to an amino acid side chain on a serine (Ser), threonine (Thr), or tyrosine (Tyr) residue of a protein, and by protein phosphatases, each of which, conversely, removes a phosphate group from a phosphoprotein. These reversible enzyme activities provide a regulatory mechanism by activating or deactivating many diverse functions of proteins in various cellular processes. In this review, their structures and substrate recognition are described and summarized, focusing on Ser/Thr protein kinases and protein Ser/Thr phosphatases, and the regulation of protein structures by phosphorylation. The studies reviewed here and the resulting information could contribute to further structural, biochemical, and combined studies on the mechanisms of protein phosphorylation and to drug discovery approaches targeting protein kinases or protein phosphatases.
Molecular-Dynamics Study on the Impact Energy Release Characteristics of Fe–Al Energetic Jets
Authors: Li Qiang , Jiang Chunlan , Du Ye
Published: September 2021

Fe–Al energetic material releases a large amount of energy under impact loading; therefore, it can replace traditional materials and be used in new weapons. This paper introduces the macroscopic experiment and microscopic molecular-dynamics simulation research on the energy release characteristics of Fe–Al energetic jets under impact loading. A macroscopic dynamic energy acquisition test system was established to quantitatively obtain the composition of Fe–Al energetic jet reaction products. A momentum mirror impacting the Fe–Al particle molecular model was established and the microstructure evolution and impact thermodynamic response of Fe–Al particles under impact loading were analyzed. The mechanism of multi-scale shock-induced chemical reaction of Fe–Al energetic jets is discussed. The results show that the difference in velocity between Fe and Al atoms at the shock wave fronts is the cause of the shock-induced reaction; when the impact strength is low, the Al particles are disordered and amorphous, while the Fe particles remain in their original state and only the oxidation reaction of Al and a small amount intermetallic compound reaction occur. With the increase of impact strength, Al particles and Fe particles are completely disordered and amorphized in a high-temperature and high-pressure environment, fully mixed and penetrated. The temperature of the system rises rapidly, due to a violent thermite reaction, and the energy released by the jet shows an increasing trend; there is an impact intensity threshold, so that the jet release energy reaches the upper limit.
Influence of Heat Treatment and Reinforcements on Tensile Characteristics of Aluminium AA 5083/Silicon Carbide/Fly Ash Composites
Authors: Nagaraja Santhosh , Kodandappa Ramesha , Ansari Khalid , Kuruniyan Mohamed Saheer , Afzal Asif , Kaladgi Abdul Razak , Aslfattahi Navid , Saleel C. Ahamed , Gowda Ashwin C. , Bindiganavile Anand Praveena
Published: September 2021

The effect of reinforcements and thermal exposure on the tensile properties of aluminium AA 5083–silicon carbide (SiC)–fly ash composites were studied in the present work. The specimens were fabricated with varying wt.% of fly ash and silicon carbide and subjected to T6 thermal cycle conditions to enhance the properties through “precipitation hardening”. The analyses of the microstructure and the elemental distribution were carried out using scanning electron microscopic (SEM) images and energy dispersive spectroscopy (EDS). The composite specimens thus subjected to thermal treatment exhibit uniform distribution of the reinforcements, and the energy dispersive spectrum exhibit the presence of Al, Si, Mg, O elements, along with the traces of few other elements. The effects of reinforcements and heat treatment on the tensile properties were investigated through a set of scientifically designed experimental trials. From the investigations, it is observed that the tensile and yield strength increases up to 160 °C, beyond which there is a slight reduction in the tensile and yield strength with an increase in temperature (i.e., 200 °C). Additionally, the % elongation of the composites decreases substantially with the inclusion of the reinforcements and thermal exposure, leading to an increase in stiffness and elastic modulus of the specimens. The improvement in the strength and elastic modulus of the composites is attributed to a number of factors, i.e., the diffusion mechanism, composition of the reinforcements, heat treatment temperatures, and grain refinement. Further, the optimisation studies and ANN modelling validated the experimental outcomes and provided the training models for the test data with the correlation coefficients for interpolating the results for different sets of parameters, thereby facilitating the fabrication of hybrid composite components for various automotive and aerospace applications.
Fabrication of Ring-Shaped Deposits of Polystyrene Microparticles Driven by Thermocapillary Mechanism
Authors: Al-Muzaiqer Mohammed , Ivanova Natalia , Klyuev Denis
Published: September 2021

Fabrication of ring-shaped deposits of microparticles on solid surfaces with the desired length scales and morphology of particle arrangements is of great importance when developing modern optical and electronic resonators, chemical sensors, touch screens, field-emission displays, porous materials, and coatings with various functional properties. However, the controlled formation of ring-shaped patterns scaling from a few millimeters up to centimeters with simultaneous control of particle arrangement at the microscale is one of the most challenging problems in advanced materials science and technology. Here, we report a fabrication approach for ring-shaped structures of microparticles on a glass surface that relied on a local thermal impact produced by the subsurface heater and heat sink. Thermocapillary convection in the liquid covering microparticles in combination with evaporative lithography is responsible for the particle transport and the assembling into the ring-shaped patterns. An advantageous feature of this approach is based on the control of thermocapillary flow direction, achieved by changing the sign of the temperature gradient in the liquid, switching between heating and cooling modes. That allows for changing the particle transfer direction to create the ring-shaped deposits and dynamically tune their size and density distribution. We have studied the influence of the power applied to the heat source/sink and the duration of the applied thermal field on the rate of the ring fabrication, the sizes of the ring and the profile of the particle distribution in the ring. The proposed method is flexible to control simultaneously the centimeter scale and microscale processes of transfer and arrangements of particles and can be applied to the fabrication of ring structures of particles of different nature and shape.
Binary Dye Removal from Simulated Wastewater Using Reduced Graphene Oxide Loaded with Fe-Cu Bimetallic Nanocomposites Combined with an Artificial Neural Network
Authors: Xin Ling , Wu Xianliang , Xiang Yiqiu , Zhang Shengsheng , Huang Xianfei , Liu Huijuan
Published: September 2021

Reduced graphene oxide loaded with an iron-copper nanocomposite was prepared in this study, using graphene oxide as a carrier and ferrous sulfate, copper chloride and sodium borohydride as raw materials. The obtained material was prepared for eliminating hazardous dye carmine and the binary dye mixture of carmine and Congo red. The process of carmine dye removal by the nanocomposite was modeled and optimized through response surface methodology and artificial intelligence (artificial neural network–particle swarm optimization and artificial neural network–genetic algorithm) based on single-factor experiments. The results demonstrated that the surface area of the nanocomposite was 41.255 m2/g, the pore size distribution was centered at 2.125 nm, and the saturation magnetization was up to 108.33 emu/g. A comparison of the material before and after the reaction showed that the material could theoretically be reused three times. The absolute error between the predicted and experimental values derived by using artificial neural network–particle swarm optimization was the smallest, indicating that this model was suitable to remove carmine from simulated wastewater. The dose factor was the key factor in the adsorption process. This process could be described with the pseudo-second-order kinetic model, and the maximum adsorption capacity was 1848.96 mg/g. The removal rate of the mixed dyes reached 96.85% under the optimal conditions (the dosage of rGO/Fe/Cu was 20 mg, the pH was equal to 4, the initial concentration of the mixed dyes was 500 mg/L, and the reaction time was 14 min), reflecting the excellent adsorption capability of the material.
A Survey on Fault Diagnosis and Fault-Tolerant Control Methods for Unmanned Aerial Vehicles
Authors: Fourlas George K. , Karras George C.
Published: September 2021

The continuous evolution of modern technology has led to the creation of increasingly complex and advanced systems. This has been also reflected in the technology of Unmanned Aerial Vehicles (UAVs), where the growing demand for more reliable performance necessitates the development of sophisticated techniques that provide fault diagnosis and fault tolerance in a timely and accurate manner. Typically, a UAV consists of three types of subsystems: actuators, main structure and sensors. Therefore, a fault-monitoring system must be specifically designed to supervise and debug each of these subsystems, so that any faults can be addressed before they lead to disastrous consequences. In this survey article, we provide a detailed overview of recent advances and studies regarding fault diagnosis, Fault-Tolerant Control (FTC) and anomaly detection for UAVs. Concerning fault diagnosis, our interest is mainly focused on sensors and actuators, as these subsystems are mostly prone to faults, while their healthy operation usually ensures the smooth and reliable performance of the aerial vehicle.
Growth and Characterisation of Antiferromagnetic Ni2MnAl Heusler Alloy Films
Authors: Huminiuc Teodor , Whear Oliver , Vick Andrew J. , Lloyd David C. , Vallejo-Fernandez Gonzalo , O’Grady Kevin , Hirohata Atsufumi
Published: September 2021

Recent rapid advancement in antiferromagnetic spintronics paves a new path for efficient computing with THz operation. To date, major studies have been performed with conventional metallic, e.g., Ir-Mn and Pt-Mn, and semiconducting, e.g., CuMnAs, antiferromagnets, which may suffer from their elemental criticality and high resistivity. In order to resolve these obstacles, new antiferromagnetic films are under intense development for device operation above room temperature. Here, we report the structural and magnetic properties of an antiferromagnetic Ni2MnAl Heusler alloy with and without Fe and Co doping in thin film form, which has significant potential for device applications.
Experimental Study on a Ceramic Membrane Condenser with Air Medium for Water and Waste Heat Recovery from Flue Gas
Authors: Teng Da , An Liansuo , Shen Guoqing , Zhang Shiping , Zhang Heng
Published: September 2021

Ceramic membrane condensers that are used for water and waste heat recovery from flue gas have the dual effects of saving water resources and improving energy efficiency. However, most ceramic membrane condensers use water as the cooling medium, which can obtain a higher water recovery flux, but the waste heat temperature is lower, which is difficult to use. This paper proposes to use the secondary boiler air as the cooling medium, build a ceramic membrane condenser with negative pressure air to recover water and waste heat from the flue gas, and analyze the transfer characteristics of flue gas water and waste heat in the membrane condenser. Based on the experimental results, it is technically feasible for the ceramic membrane condenser to use negative pressure air as the cooling medium. The flue gas temperature has the most obvious influence on the water and heat transfer characteristics. The waste heat recovery is dominated by latent heat of water vapor, accounting for 80% or above. The negative pressure air outlet temperature of the ceramic membrane condenser can reach 50.5 °C, and it is in a supersaturated state. The research content of this article provides a new idea for the water and waste heat recovery from flue gas.