bluuebunny/crossref_metadata_2025 huggingface dataset:
Rifampicin–Liposomes for Mycobacterium abscessus Infection Treatment: Intracellular Uptake and Antibacterial Activity Evaluation
Authors: Rinaldi Federica , Hanieh Patrizia Nadia , Sennato Simona , De Santis Federica , Forte Jacopo , Fraziano Maurizio , Casciardi Stefano , Marianecci Carlotta , Bordi Federico , Carafa Maria
Published: July 2021

Treatment of pulmonary infections caused by Mycobacterium abscessus are extremely difficult to treat, as this species is naturally resistant to many common antibiotics. Liposomes are vesicular nanocarriers suitable for hydrophilic and lipophilic drug loading, able to deliver drugs to the target site, and successfully used in different pharmaceutical applications. Moreover, liposomes are biocompatible, biodegradable and nontoxic vesicles and nebulized liposomes are efficient in targeting antibacterial agents to macrophages. The present aim was to formulate rifampicin-loaded liposomes (RIF–Lipo) for lung delivery, in order to increase the local concentration of the antibiotic. Unilamellar liposomal vesicles composed of anionic DPPG mixed with HSPC for rifampicin delivery were designed, prepared, and characterized. Samples were prepared by using the thin-film hydration method. RIF–Lipo and unloaded liposomes were characterized in terms of size, ζ-potential, bilayer features, stability and in different biological media. Rifampicin’s entrapment efficiency and release were also evaluated. Finally, biological activity of RIF-loaded liposomes in Mycobacterium abscessus-infected macrophages was investigated. The results show that RIF-lipo induce a significantly better reduction of intracellular Mycobacterium abscessus viability than the treatment with free drug. Liposome formulation of rifampicin may represent a valuable strategy to enhance the biological activity of the drug against intracellular mycobacteria.
An Updated Risk Assessment as Part of the QbD-Based Liposome Design and Development
Authors: Németh Zsófia , Pallagi Edina , Dobó Dorina Gabriella , Kozma Gábor , Kónya Zoltán , Csóka Ildikó
Published: July 2021

Liposomal formulation development is a challenging process. Certain factors have a critical influence on the characteristics of the liposomes, and even the relevant properties can vary based on the predefined interests of the research. In this paper, a Quality by Design-guided and Risk Assessment (RA)-based study was performed to determine the Critical Material Attributes and the Critical Process Parameters of an “intermediate” active pharmaceutical ingredient-free liposome formulation prepared via the thin-film hydration method, collect the Critical Quality Attributes of the future carrier system and show the process of narrowing a general initial RA for a specific case. The theoretical liposome design was proved through experimental models. The investigated critical factors covered the working temperature, the ratio between the wall-forming agents (phosphatidylcholine and cholesterol), the PEGylated phospholipid content (DPPE-PEG2000), the type of the hydration media (saline or phosphate-buffered saline solutions) and the cryoprotectants (glucose, sorbitol or trehalose). The characterisation results (size, surface charge, thermodynamic behaviours, formed structure and bonds) of the prepared liposomes supported the outcomes of the updated RA. The findings can be used as a basis for a particular study with specified circumstances.
Two in One: Use of Divalent Manganese Ions as Both Cross-Linking and MRI Contrast Agent for Intrathecal Injection of Hydrogel-Embedded Stem Cells
Authors: Kalkowski Lukasz , Golubczyk Dominika , Kwiatkowska Joanna , Holak Piotr , Milewska Kamila , Janowski Miroslaw , Oliveira Joaquim Miguel , Walczak Piotr , Malysz-Cymborska Izabela
Published: July 2021

Cell therapy is a promising tool for treating central nervous system (CNS) disorders; though, the translational efforts are plagued by ineffective delivery methods. Due to the large contact surface with CNS and relatively easy access, the intrathecal route of administration is attractive in extensive or global diseases such as stroke or amyotrophic lateral sclerosis (ALS). However, the precision and efficacy of this approach are still a challenge. Hydrogels were introduced to minimize cell sedimentation and improve cell viability. At the same time, contrast agents were integrated to allow image-guided injection. Here, we report using manganese ions (Mn2+) as a dual agent for cross-linking alginate-based hydrogels and magnetic resonance imaging (MRI). We performed in vitro studies to test the Mn2+ alginate hydrogel formulations for biocompatibility, injectability, MRI signal retention time, and effect on cell viability. The selected formulation was injected intrathecally into pigs under MRI control. The biocompatibility test showed a lack of immune response, and cells suspended in the hydrogel showed greater viability than monolayer culture. Moreover, Mn2+-labeled hydrogel produced a strong T1 MRI signal, which enabled MRI-guided procedure. We confirmed the utility of Mn2+ alginate hydrogel as a carrier for cells in large animals and a contrast agent at the same time.
Novel Non-Congeneric Derivatives of the Choline Kinase Alpha Inhibitor ICL-CCIC-0019
Authors: Wang Ning , Brickute Diana , Braga Marta , Barnes Chris , Lu Haonan , Allott Louis , Aboagye Eric O.
Published: July 2021

Choline kinase alpha (CHKA) is a promising target for the development of cancer therapeutics. We have previously reported ICL-CCIC-0019, a potent CHKA inhibitor with high cellular activity but with some unfavorable pharmacological properties. In this work, we present an active analogue of ICL-CCIC-0019 bearing a piperazine handle (CK146) to facilitate further structural elaboration of the pharmacophore and thus improve the biological profile. Two different strategies were evaluated in this study: (1) a prodrug approach whereby selective CHKA inhibition could be achieved through modulating the activity of CK146, via the incorporation of an ε-(Ac) Lys motif, cleavable by elevated levels of histone deacetylase (HDAC) and cathepsin L (CTSL) in tumour cells; (2) a prostate-specific membrane antigen (PSMA) receptor targeted delivery strategy. Prodrug (CK145) and PSMA-targeted (CK147) derivatives were successfully synthesized and evaluated in vitro. While the exploitation of CK146 in those two strategies did not deliver the expected results, important and informative structure-activity relationships were observed and have been reported.
In Vitro Evaluation of Nasal Aerosol Depositions: An Insight for Direct Nose to Brain Drug Delivery
Authors: Maaz Aida , Blagbrough Ian S. , De Bank Paul A.
Published: July 2021

The nasal cavity is an attractive route for both local and systemic drug delivery and holds great potential for access to the brain via the olfactory region, an area where the blood–brain barrier (BBB) is effectively absent. However, the olfactory region is located at the roof of the nasal cavity and only represents ~5–7% of the epithelial surface area, presenting significant challenges for the deposition of drug molecules for nose to brain drug delivery (NTBDD). Aerosolized particles have the potential to be directed to the olfactory region, but their specific deposition within this area is confounded by a complex combination of factors, which include the properties of the formulation, the delivery device and how it is used, and differences in inter-patient physiology. In this review, an in-depth examination of these different factors is provided in relation to both in vitro and in vivo studies and how advances in the fabrication of nasal cast models and analysis of aerosol deposition can be utilized to predict in vivo outcomes more accurately. The challenges faced in assessing the nasal deposition of aerosolized particles within the paediatric population are specifically considered, representing an unmet need for nasal and NTBDD to treat CNS disorders.
The International Pharmacy Game: A Comparison of Implementation in Seven Universities World-Wide
Authors: Fens Tanja , Hope Denise L. , Crawshaw Sarah , Tommelein Eline , Dantuma-Wering Claudia , Verdel Bertha Maria , Trečiokienė Indrė , Solanki Vibhu , van Puijenbroek Eugène P. , Taxis Katja
Published: July 2021

The utilization of serious games and simulations in health professional education has increased. The Pharmacy Game is one such concept that intersects gamification and simulation, in which pharmacy student teams competitively manage simulated pharmacies; a concept included in the pharmacy curricula of seven international universities. This study aimed to compare the implementation and conduct of the Pharmacy Game of participant universities and their students’ performance in the same educational task. Data were collected via a questionnaire completed by academic staff in April 2020, and the collation of results of the same patient case was conducted at each university (April 2020 to March 2021). The main results reflected differences in the game frequencies and the curricular approach (standalone or integrated course) and in the learning outcomes for the Pharmacy Game. Other differences were identified in the extent to which students of other professions were part of the game such as medical students or pharmacy assistants. Student case outcomes revealed similar strengths across the universities in patient communication and focus on safety, with variations identified as areas for improvement. Collation of the international utilization of the Pharmacy Game identified a broad spectrum of similar learning outcomes, inspiring a model of international core and aspirational learning outcomes. While the Pharmacy Game has been implemented with flexibility regarding the numbers of teams (4–10) and the duration of activity (12–36 days), all universities reported positive experiences and student outcomes, suggesting that the intervention represents a potential tool to deliver capstone learning experiences, promote interprofessional education, reinforce patient safety, and prepare pharmacy graduates for future practice.
Visual Adaptation to Scattering in Myopes
Authors: Villa-Carpes José A. , Bueno Juan M. , Fernández Enrique J.
Published: July 2021

Myopes exhibit a larger capability of adaptation to defocus. Adaptation produces a boost in visual performance that can be characterized through different metrics. The ability of myopes to adapt to other sources of blur, such as diffusion, has not been studied so far. In this work, a group of 20 myopes with normal vision underwent high-contrast visual acuity (VA) measurements under different viewing conditions, wearing their refractive correction with or without a diffuser (Bangerter filter, BF). VA decreased immediately after wearing the BF of density 0.6, showing a significant relationship with the ocular refraction. After 40 minutes of binocular vision through the BF, a statistically significant increase (p = 0.02) in VA from 0.54 to 0.62 in decimal scale (from 0.3 to 0.2 logMAR) was obtained. No correlation with the refraction was observed. After removing the diffuser, VA returned to baseline. A control group (17 subjects) underwent the same experimental protocol but without diffuser filters. No significant changes in VA were found in this group. We describe a new type of contrast adaptation to blur in myopes caused by scattering, rather than by defocus. The effects of low scattering levels in vision might be relevant in the analysis of early stage of cataract, amblyopia treatments, and myopia understanding.
Morphological Formation, Fatty Acid Profile, and Molecular Identification of Some Landraces of Ethiopian Brassica as a Promising Crop to Support Breeding Programs
Authors: Khalaf Ahmed E. A. , Abd Al-Aziz Samia A. , Ali Safaa M. , Mohdaly Adel A. , Rady Mostafa M. , Majrashi Ali , Ali Esmat F. , Yassein Ahmed A. M.
Published: July 2021

There has been an increased interest in oilseed crops for agro-industry research and development breeding programs to secure sustainable food and agriculture. The introgression of exotic genotypes of oilseed Brassica into cultivated relatives is inevitable in the genetic improvement of oilseed crops. This experimental attempt aimed to characterize the morphological and molecular basis for the identification and characterization of some Brassica genotypes. Fatty acid profile, yield, and morphology are under genetic control and can be used to identify genotypes. Characterization and identification were fulfilled for five accessions from Brassica spp. Plant height, height of first branch, number of branches and pods per plant, seed yield per plant, average pod length, number of seeds per pod, protein and oil contents (%), and fatty acid profile were examined. Besides, the relationship between seed yield and seed yield-contributing characteristics was estimated, as well as the phylogenetic relationship of the internal transcribed spacer (ITS). The genotypes varied significantly for all examined traits, taking into account the most important traits: seed yield per plant and oil content. For example, oil content in the samples ranged between 41.1 and 49.3%. Path analysis results showed a high and positive direct effect between each number of primary branches and the number of pods per plant with seed yield per plant (0.48). The morphological and molecular observations suggest that the Fay1, Fay3, Fay4, and Fay6 accessions belong to Brassica rapa, while Fay2 belongs to Brassica carinata. It can be concluded based on the present findings that the Fay3 genotype with the highest oil content and the lowest erucic acid content compared to the other genotypes can be proposed as a potential donor for future breeding programs for oil production and quality, while Fay1 can be utilized as donor to increase the seed yield per plant.
Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan
Authors: Ariyawansa Hiran A. , Tsai Ichen , Wang Jian-Yuan , Withee Patchareeya , Tanjira Medsaii , Lin Shiou-Ruei , Suwannarach Nakarin , Kumla Jaturong , Elgorban Abdallah M. , Cheewangkoon Ratchadawan
Published: July 2021

Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.
Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-co-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques
Authors: Chen Yi-Pin , Lo Tsia-Shu , Lin Yu-Ting , Chien Yu-Han , Lu Chia-Jung , Liu Shih-Jung
Published: July 2021

We developed biodegradable drug-eluting prolapse mats using solution-extrusion 3D printing and coaxial electrospinning techniques. The mats were composed of polycaprolactone (PCL) mesh and lidocaine-, estradiol-, metronidazole-, and connective tissue growth factor (CTGF)-incorporated poly(lactic-co-glycolic acid) (PLGA) nanofibers that mimic the structure of the natural extracellular matrix of most connective tissues. The mechanical properties of degradable prolapse membrane were assessed and compared to commercial non-degradable polypropylene knitted meshes clinically used for pelvic organ prolapse (POP) repair. The release behaviors of the drug-loaded hybrid degradable membranes were also characterized. The experimental results suggest that 3D-printed PCL meshes exhibited comparable strengths to commercial POP meshes and survived through 10,000 cycles of fatigue test without breakage. Hybrid PCL meshes/PLGA nanofibrous membranes provided a sustainable release of metronidazole, lidocaine, and estradiol for 4, 25, and 30 days, respectively, in vitro. The membranes further liberated high levels of CTGF for more than 30 days. The animal tests show that the mechanical property of PCL mesh decreased with time, mainly due to degradation of the polymers post-implantation. No adverse effect of the mesh/nanofibers was noted in the histological images. By adopting solution-extrusion 3D printing and coaxial electrospinning, degradable drug-eluting membranes can be fabricated for POP applications.
Influence of Whey Protein Micro-Gel Particles and Whey Protein Micro-Gel Particles-Xanthan Gum Complexes on the Stability of O/W Emulsions
Authors: Zhang Man , Liang Bin , He Hongjun , Ji Changjian , Cui Tingting , Sun Chanchan
Published: July 2021

Appropriate pretreatment of proteins and addition of xanthan gum (XG) has the potential to improve the stability of oil-in-water (O/W) emulsions. However, the factors that regulate the enhancement and the mechanism are still not clear, which restricts the realization of improving the emulsion stability by directional design of its structure. Therefore, the effects of whey protein micro-gel particles (WPMPs) and WPMPs-XG complexes on the stability of O/W emulsion were investigated in this article to provide theoretical support. WPMPs with different structures were prepared by pretreatment (controlled high-speed shear treatment of heat-set WPC gels) at pH 3.5–8.5. The impact of initial WPC structure and XG addition on Turbiscan Indexes, mean droplet size and the peroxide values of O/W emulsions was investigated. The results indicate that WPMPs and XG can respectively inhibit droplet coalescence and gravitational separation to improve the physical stability of WPC-stabilized O/W emulsions. The pretreatment significantly enhanced the oxidative stability of WPC-stabilized O/W emulsions. The addition of XG did not necessarily enhance the oxidative stability of O/W emulsions. Whether the oxidative stability of the O/W emulsion with XG is increased or decreased depends on the interface structure of the protein-XG complex. This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
Bionanocarbon Functional Material Characterisation and Enhancement Properties in Nonwoven Kenaf Fibre Nanocomposites
Authors: Rizal Samsul , Mistar E. M. , Rahman A. A. , H.P.S. Abdul Khalil , Oyekanmi A. A. , Olaiya N. G. , Abdullah C. K. , Alfatah Tata
Published: July 2021

Bionanocarbon as a properties enhancement material in fibre reinforced nanobiocomposite was investigated for sustainable material applications. Currently, an extensive study using the micro size of biocarbon as filler or reinforcement materials has been done. However, poor fibre-matrix interface results in poor mechanical, physical, and thermal properties of the composite. Hence in this study, the nanoparticle of biocarbon was synthesised and applied as a functional material and properties enhancement in composite material. The bionanocarbon was prepared from an oil palm shell, an agriculture waste precursor, via a single-step activation technique. The nanocarbon filler loading was varied from 0, 1, 3, and 5% as nanoparticle properties enhancement in nonwoven kenaf fibre reinforcement in vinyl ester composite using resin transfer moulding technique. The functional properties were evaluated using TEM, particle size, zeta potential, and energy dispersion X-ray (EDX) elemental analysis. While the composite properties enhancement was evaluated using physical, mechanical, morphological, thermal, and wettability properties. The result indicated excellent nanofiller enhancement of fibre-matrix bonding that significantly improved the physical, mechanical, and thermal properties of the bionanocomposite. The SEM morphology study confirmed the uniform dispersion of the nanoparticle enhanced the fibre-matrix interaction. In this present work, the functional properties of bionanocarbon from oil palm shells (oil palm industrial waste) was incorporated in nanaobiocomposite, which significantly enhance its properties. The optimum enhancement of the bionanocomposite functional properties was obtained at 3% bionanocarbon loading. The improvement can be attributed to homogeneity and improved interfacial interaction between nanoparticles, kenaf fibre, and matrix.
Synthesis of New Chitosan from an Endemic Chilean Crayfish Exoskeleton (Parastacus Pugnax): Physicochemical and Biological Properties
Authors: Burgos-Díaz César , Opazo-Navarrete Mauricio , Palacios José Luis , Barahona Tamara , Mosi-Roa Yohanna , Anguita-Barrales Fresia , Bustamante Mariela
Published: July 2021

Chitin is one of the most abundant natural polysaccharides in the world and it is mainly used to produce chitosan by a deacetylation process. In the present study, the extraction of chitin and chitosan from the Parastacus pugnax (P. pugnax) crayfish exoskeleton was studied for the first time. Thus, the P. pugnax crayfish exoskeleton was converted to chitosan following the steps of depigmentation, deproteinization, and deacetylation. The produced chitosan (Chitosan-CGNA) was characterized in terms of the protein content, solubility, degree of deacetylation, viscosity, molecular weight, FTIR, SEM, XRD, antimicrobial, and antioxidant activity. The results showed that the obtained chitosan had a high degree of deacetylation (91.55%) and a medium molecular weight (589.43 kDa). The antibacterial activity of the chitosan was tested against bacterial strains relevant for the food industry and the lowest minimum inhibitory concentration (MIC) values were evidenced with Salmonella tiphymurium (S. typhimurium), Staphylococcus aureus (S. aureus), Enterococcus faecalis (E. faecalis) and Listeria. Monocytogenes (L. monocytogenes). Moreover, the Chitosan-CGNA showed an effect on DPPH radical scavenging activity, and its antioxidant activity was dependent on concentration and deacetylation degree. These results suggest that P. pugnax exoskeleton could be an excellent natural source for the production of chitosan with potential applications in the health system, and to prevent infections associated with pathogens strains.
Metal Organic Frameworks Derived Sustainable Polyvinyl Alcohol/Starch Nanocomposite Films as Robust Materials for Packaging Applications
Authors: Khan Naveed Ahmed , Niazi Muhammad Bilal Khan , Sher Farooq , Jahan Zaib , Noor Tayyaba , Azhar Ofaira , Rashid Tazien , Iqbal Naseem
Published: July 2021

Bio-nanocomposites-based packaging materials have gained significance due to their prospective application in rising areas of packaged food. This research aims to fabricate biodegradable packaging films based upon polyvinyl alcohol (PVA) and starch integrated with metal-organic frameworks (MOFs) or organic additives. MOFs offer unique features in terms of surface area, mechanical strength, and chemical stability, which make them favourable for supporting materials used in fabricating polymer-based packaging materials. zeolitic imidazolate frameworks (ZIFs) are one of the potential candidates for this application due to their highly conductive network with a large surface area and high porosity. Present research illustrates a model system based on ZIF-67 (C8H10N4Co) bearing 2–10 wt.% loading in a matrix of PVA/starch blend with or without pyrolysis to probe the function of intermolecular interaction in molecular packing, tensile properties, and glass transition process. ZIF-67 nanoparticles were doped in a PVA/starch mixture, and films were fabricated using the solution casting method. It was discovered through scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR) that addition of ZIF-67 and pyrolyzed ZIF-67 changed and enhanced the thermal stability of the membrane. Moreover, 2–10 wt.% loading of ZIF-67 effected the thermal stability, owing to an interlayer aggregation of ZIF-67. The membranes containing pyrolyzed ZIF-67 showed mechanical strength in the order of 25 MPa in a moderate loading of pyrolyzed ZIF-67 (i.e., at 4 wt.%). The crystallinity enhanced by an increment in ZIF-67 loading. On the other hand, pyrolyzed ZIF-67 carbon became amorphous because of the inert environment and elevated temperature. The surface area also increased after the pyrolysis, which helped to increase the strength of the composite films.
Pressure Equilibrium Time of a Cyclic-Olefin Copolymer
Authors: Roth Benedikt , Drummer Dietmar
Published: July 2021

Integrative simulation techniques for predicting component properties, based on the conditions during processing, are becoming increasingly important. The calculation of orientations in injection molding, which, in addition to mechanical and optical properties, also affect the thermal shrinkage behavior, are modeled on the basis of measurements that cannot take into account the pressure driven flow processes, which cause the orientations during the holding pressure phase. Previous investigations with a high-pressure capillary rheometer (HPC) and closed counter pressure chamber (CPC) showed the significant effect of a dynamically applied pressure on the flow behavior, depending on the temperature and the underlying compression rate. At a constant compression rate, an effective pressure difference between the measuring chamber and the CPC was observed, which resulted in a stop of flow through the capillary referred to as dynamic compression induced solidification. In order to extend the material understanding to the moment after dynamic solidification, an equilibrium time, which is needed until the pressure signals equalize, was evaluated and investigated in terms of a pressure, temperature and a possible compression rate dependency in this study. The findings show an exponential increase of the determined equilibrium time as a function of the holding pressure level and a decrease of the equilibrium time with increasing temperature. In case of supercritical compression in the area of a dynamic solidification, a compression rate dependency of the determined equilibrium times is also found. The measurement results show a temperature-invariant behavior, which allows the derivation of a master curve, according to the superposition principle, to calculate the pressure equilibrium time as a function of the holding pressure and the temperature.
Development and Characterization of Yeast-Incorporated Antimicrobial Cellulose Biofilms for Edible Food Packaging Application
Authors: Atta Omar Mohammad , Manan Sehrish , Ahmed Abeer Ahmed Qaed , Awad Mohamed F. , Ul-Islam Mazhar , Subhan Fazli , Ullah Muhammad Wajid , Yang Guang
Published: July 2021

The unique properties and advantages of edible films over conventional food packaging have led the way to their extensive exploration in recent years. Moreover, the incorporation of bioactive components during their production has further enhanced the intrinsic features of packaging materials. This study was aimed to develop edible and bioactive food packaging films comprising yeast incorporated into bacterial cellulose (BC) in conjunction with carboxymethyl cellulose (CMC) and glycerol (Gly) to extend the shelf life of packaged food materials. First, yeast biomass and BC hydrogels were produced by Meyerozyma guilliermondii (MT502203.1) and Gluconacetobacter xylinus (ATCC53582), respectively, and then the films were developed ex situ by mixing 30 wt.% CMC, 30 wt.% Gly, 2 wt.% yeast dry biomass, and 2 wt.% BC slurry. FE-SEM observation showed the successful incorporation of Gly and yeast into the fibrous cellulose matrix. FTIR spectroscopy confirmed the development of composite films through chemical interaction between BC, CMC, Gly, and yeast. The developed BC/CMC/Gly/yeast composite films showed high water solubility (42.86%). The yeast-incorporated films showed antimicrobial activities against three microbial strains, including Escherichia coli, Pseudomonas aeruginosa, and Saccharomyces aureus, by producing clear inhibition zones of 16 mm, 10 mm, and 15 mm, respectively, after 24 h. Moreover, the films were non-toxic against NIH-3T3 fibroblast cells. Finally, the coating of oranges and tomatoes with BC/CMC/Gly/yeast composites enhanced the shelf life at different storage temperatures. The BC/CMC/Gly/yeast composite film-coated oranges and tomatoes demonstrated acceptable sensory features such as odor and color, not only at 6 °C but also at room temperature and further elevated temperatures at 30 °C and 40 °C for up to two weeks. The findings of this study indicate that the developed BC/CMC/Gly/yeast composite films could be used as edible packaging material with high nutritional value and distinctive properties related to the film component, which would provide protection to foods and extend their shelf life, and thus could find applications in the food industry.
Biological Activity of Propolis Ointment with the Addition of 1% Nanosilver in the Treatment of Experimentally-Evoked Burn Wounds
Authors: Staniczek Jakub , Jastrzębska-Stojko Żaneta , Stojko Rafał
Published: July 2021

The main objective of this study was to assess the pharmacological efficacy of ointments containing 1% propolis and 1% nanosilver, compared to the conventional treatment of burn wounds. In the evaluation of the results, we used clinical observation of scars, microbiological examinations, pathomorphological examinations, and analysis of free radicals. The analysis of the experiment results concerning the therapeutic effectiveness of the propolis ointment revealed its wide-ranging antibacterial action (against Gram-positive and Gram-negative bacteria). The 1% propolis ointment was found to accelerate neoangiogenesis and epithelialization, have a positive effect on the healing of burn wounds, improve the cosmetic look of scars, and have no side-effects. The analysis of free radicals in burn wounds showed impressive activity of the 1% nanosilver ointment in the reduction of free radicals. No synergism of pharmacological activity of propolis and nanosilver was shown. A comparative evaluation of the acquired research material allows us to provide a favorable opinion on the topical treatment of burn wounds with 1% propolis. The obtained results show that the 1% propolis ointment reduces healing time, offers antimicrobial action, and has a positive effect on the normal process of scar formation.
Lyophilized Composite Loaded with Meloxicam-Peppermint oil Nanoemulsion for Periodontal Pain
Authors: Sindi Amal M. , Hosny Khaled M. , Alharbi Waleed S.
Published: July 2021

Maintaining oral health helps to prevent periodontal inflammation and pain, which can progress into more detrimental issues if left untreated. Meloxicam (MX) is a commonly used analgesic for periodontal pain, but it can have adverse gastrointestinal effects and poor solubility. Therefore, this study aimed to enhance the solubility of MX by developing a self-nanoemulsifying drug delivery system (SNEDDS). Considering the anti-ulcer activity of peppermint oil (PO), it was added in a mixture with medium-chain triglyceride (MCT) to the MX-loaded SNEDDS formulation (MX-PO-SNEDDS). After optimization, MX-PO-SNEDDS exhibited a PO:MCT ratio of 1.78:1, surfactant mixture HLB value of 14, and MX:oil mix ratio of 1:15, a particle size of 47 ± 3 nm, stability index of 85 ± 4%, ex vivo Jss of 4 ± 0.6 μg/cm2min, and ulcer index of 1 ± 0.25 %. Then, orally flash disintegrating lyophilized composites (MX-SNELCs) were prepared using the optimized MX-PO-SNEDDs. Results reveal that MX-SNELCs had a wetting time of 4 ± 1 s and disintegration time of 3 ± 1 s with a high in vitro MX release of 91% by the end of 60 min. The results of pharmacokinetic studies in human volunteers further demonstrated that, compared to a marketed MX tablets, MX-SNELCs provided a higher Cmax, Tmax, and AUC and a relatively greater bioavailability of 152.97 %. The successfully developed MX-SNELCs were found to be a better alternative than the conventional tablet dosage form, thus indicating their potential for further development in a clinically acceptable strategy for managing periodontal pain.
Phlogopite-Reinforced Natural Rubber (NR)/Ethylene-Propylene-Diene Monomer Rubber (EPDM) Composites with Aminosilane Compatibilizer
Authors: Lee Sung-Hun , Park Su-Yeol , Chung Kyung-Ho , Jang Keon-Soo
Published: July 2021

Rubber compounding with two or more components has been extensively employed to improve various properties. In particular, natural rubber (NR)/ethylene-propylene-diene monomer rubber (EPDM) blends have found use in tire and automotive parts. Diverse fillers have been applied to NR/EPDM blends to enhance their mechanical properties. In this study, a new class of mineral filler, phlogopite, was incorporated into an NR/EPDM blend to examine the mechanical, curing, elastic, and morphological properties of the resulting material. The combination of aminoethylaminopropyltrimethoxysilane (AEAPS) and stearic acid (SA) compatibilized the NR/EPDM/phlogopite composite, further improving various properties. The enhanced properties were compared with those of NR/EPDM/fillers composed of silica or carbon black (CB). Compared with the NR/EPDM/silica composite, the incompatibilized NR/EPDM/phlogopite composite without AEAPS exhibited poorer properties, but NR/EPDM/phlogopite compatibilized by AEAPS and SA showed improved properties. Most properties of the compatibilized NR/EPDM/phlogopite composite were similar to those of the NR/EPDM/CB composite, except for the lower abrasion resistance. The NR/EPDM/phlogopite/AEAPS rubber composite may potentially be used in various applications by replacing expensive fillers, such as CB.
The Regulation and Role of piRNAs and PIWI Proteins in Cancer
Authors: Jeong Hyeseon , Park Kyung Hwan , Lee Yuri , Jeong Ayoung , Choi Sooji , Kim Kyung Won
Published: July 2021

P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are regulatory small non-coding RNAs that participate in transposon inactivation, chromatin regulation, and endogenous gene regulation. Numerous genetic and epigenetic factors regulate cell proliferation and tumor metastasis. PIWI proteins and piRNAs have been revealed to function in regulating upstream or downstream of oncogenes or tumor-suppressor genes in cancer tissues. In the present review, we summarize major recent findings in uncovering the regulation and role of PIWI proteins and piRNAs in tumorigenesis and highlight some of the promising applications of specific piRNAs in cancer therapeutics and as cancer biomarkers.
A Novel Manganese-Rich Pokeweed Biochar for Highly Efficient Adsorption of Heavy Metals from Wastewater: Performance, Mechanisms, and Potential Risk Analysis
Authors: Yang Zhe , Hu Wenyong , Yao Bin , Shen Liangchen , Jiang Feifeng , Zhou Yaoyu , Núñez-Delgado Avelino
Published: July 2021

A novel manganese-rich pokeweed biochar was prepared at different temperatures from manganese-rich pokeweed plants collected at manganese tailings, resulting in materials identified as BC300, BC400, and BC500. The synthetized biochar materials were investigated as regards their potential for removing Cu2+, Pb2+, and Cd2+, specifically in terms of adsorption performances, adsorption kinetics, adsorption isotherms, and potential environmental pollution risk. The results showed that the sorption process fitted well to the pseudo-second-order kinetic and Langmuir models, and the maximum adsorption capacities of BC500 were 246, 326, and 310 mg·g−1 for Cu2+, Pb2+, and Cd2+ respectively. The physicochemical characteristics of the biochars, and the adsorption mechanisms, were revealed by using scanning electron microscopy-energy spectrometer, elemental analysis, Brunauer–Emmett–Teller techniques, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The sorption mechanism of these three heavy metal ions onto biochars included ion exchange, electrostatic adsorption, chemical adsorption, and precipitation. Besides, the potential pollution risk of manganese-rich pokeweed biochars was significantly reduced after pyrolysis. Therefore, it is feasible to transform manganese-rich pokeweed biomass into manganese-rich pokeweed biochar with potential for heavy metals removal, showing high adsorption capacity, recyclability, and low environmental pollution.
Production of a PET//LDPE Laminate Using a Reversibly Crosslinking Packaging Adhesive and Recycling in a Small-Scale Technical Plant
Authors: Kaiser Katharina M. A. , Ginzinger Tanja
Published: July 2021

Multilayer packaging is an important part of the packaging market, but it is not recyclable with conventional methods since it is made of different thermodynamically immiscible materials. In this work, it was shown that it is possible to produce a PET//LDPE laminate in a pilot plant for lamination by using an adhesive consisting of maleimide- and furan-functionalized polyurethane prepolymers that cure through the Diels–Alder reaction. The material could then be delaminated in a small-scale recycling plant using a solvent-based recycling process by partially opening the Diels–Alder adducts through the influence of temperature. The PET and LDPE could be recovered without any adhesive residues before each material was regranulated, and in the case of the PE, a film was produced via cast film extrusion. The obtained PET granulate exhibited a slight, approximately 10%, decrease in molecular weight. However, since small amounts of LDPE could not be separated, compatibilization would still be required here for further use of the material. The obtained LDPE film was characterized by means of infrared spectrometry, differential scanning calorimetry, tensile testing, determination of the melt index, and molecular weight. The film showed lower crosslinking than usual for LDPE recycling and exhibited good mechanical properties. In this work, it was thus shown that upscaling of the laminate production with the modified adhesive and also its recycling at the pilot plant scale is possible and thus could be an actual option for recycling multilayer packaging.
A Gap Analysis of Ship-Recycling Practices in Indonesia
Authors: Sunaryo Sunaryo , Djatmiko Eko , Fariya Siti , Kurt Rafet , Gunbeyaz Sefer
Published: July 2021

Ship recycling is gaining attention in Indonesia due to the increase in end-of-life ships and uneconomical nationally flagged ships, and is considered a prospective source of economic development and employment opportunity, and yet conceivably poses a threat to the health and safety of workers and the environment. There are international and national regulations that govern ship-recycling activities to ensure that the hazardous impacts of the industry are minimized. We investigated the disparity between current ship-breaking practices in Indonesia and the requirements of related international and national regulations, with the findings intended for use as a stepping stone to proposing a strategy to establish a green and sustainable ship-recycling industry. A benchmark study of the world’s leading ship-recycling countries was conducted, and a gap analysis was performed by comparing existing international and national regulations with current ship-breaking practices in Indonesia. We identified two types of ship-breaking practices in Indonesia: Conventional environmentally unfriendly ship-breaking method, conducted by most Indonesian ship-breaking yards, and a rather modern, more environmentally friendly method, conducted by ship-repair yards. However, neither of the practices met the requirements of the regulations, and improvements are therefore needed to make the ship-recycling industry more green and sustainable, and to gain international recognition.
Mathematical Model for Choosing Counterparty When Assessing Information Security Risks
Authors: Koltays Andrey , Konev Anton , Shelupanov Alexander
Published: July 2021

The need to assess the risks of the trustworthiness of counterparties is increasing every year. The identification of increasing cases of unfair behavior among counterparties only confirms the relevance of this topic. The existing work in the field of information and economic security does not create a reasonable methodology that allows for a comprehensive study and an adequate assessment of a counterparty (for example, a developer company) in the field of software design and development. The purpose of this work is to assess the risks of a counterparty’s trustworthiness in the context of the digital transformation of the economy, which in turn will reduce the risk of offenses and crimes that constitute threats to the security of organizations. This article discusses the main methods used in the construction of a mathematical model for assessing the trustworthiness of a counterparty. The main difficulties in assessing the accuracy and completeness of the model are identified. The use of cross-validation to eliminate difficulties in building a model is described. The developed model, using machine learning methods, gives an accurate result with a small number of compared counterparties, which corresponds to the order of checking a counterparty in a real system. The results of calculations in this model show the possibility of using machine learning methods in assessing the risks of counterparty trustworthiness.
Assessing Spatial Variability of Barley Whole Crop Biomass Yield and Leaf Area Index in Silvoarable Agroforestry Systems Using UAV-Borne Remote Sensing
Authors: Wengert Matthias , Piepho Hans-Peter , Astor Thomas , Graß Rüdiger , Wijesingha Jayan , Wachendorf Michael
Published: July 2021

Agroforestry systems (AFS) can provide positive ecosystem services while at the same time stabilizing yields under increasingly common drought conditions. The effect of distance to trees in alley cropping AFS on yield-related crop parameters has predominantly been studied using point data from transects. Unmanned aerial vehicles (UAVs) offer a novel possibility to map plant traits with high spatial resolution and coverage. In the present study, UAV-borne red, green, blue (RGB) and multispectral imagery was utilized for the prediction of whole crop dry biomass yield (DM) and leaf area index (LAI) of barley at three different conventionally managed silvoarable alley cropping agroforestry sites located in Germany. DM and LAI were modelled using random forest regression models with good accuracies (DM: R² 0.62, nRMSEp 14.9%, LAI: R² 0.92, nRMSEp 7.1%). Important variables for prediction included normalized reflectance, vegetation indices, texture and plant height. Maps were produced from model predictions for spatial analysis, showing significant effects of distance to trees on DM and LAI. Spatial patterns differed greatly between the sampled sites and suggested management and soil effects overriding tree effects across large portions of 96 m wide crop alleys, thus questioning alleged impacts of AFS tree rows on yield distribution in intensively managed barley populations. Models based on UAV-borne imagery proved to be a valuable novel tool for prediction of DM and LAI at high accuracies, revealing spatial variability in AFS with high spatial resolution and coverage.
Aboveground Biomass Mapping of Crops Supported by Improved CASA Model and Sentinel-2 Multispectral Imagery
Authors: Fang Peng , Yan Nana , Wei Panpan , Zhao Yifan , Zhang Xiwang
Published: July 2021

The net primary productivity (NPP) and aboveground biomass mapping of crops based on remote sensing technology are not only conducive to understanding the growth and development of crops but can also be used to monitor timely agricultural information, thereby providing effective decision making for agricultural production management. To solve the saturation problem of the NDVI in the aboveground biomass mapping of crops, the original CASA model was improved using narrow-band red-edge information, which is sensitive to vegetation chlorophyll variation, and the fraction of photosynthetically active radiation (FPAR), NPP, and aboveground biomass of winter wheat and maize were mapped in the main growing seasons. Moreover, in this study, we deeply analyzed the seasonal change trends of crops’ biophysical parameters in terms of the NDVI, FPAR, actual light use efficiency (LUE), and their influence on aboveground biomass. Finally, to analyze the uncertainty of the aboveground biomass mapping of crops, we further discussed the inversion differences of FPAR with different vegetation indices. The results demonstrated that the inversion accuracies of the FPAR of the red-edge normalized vegetation index (NDVIred-edge) and red-edge simple ratio vegetation index (SRred-edge) were higher than those of the original CASA model. Compared with the reference data, the accuracy of aboveground biomass estimated by the improved CASA model was 0.73 and 0.70, respectively, which was 0.21 and 0.13 higher than that of the original CASA model. In addition, the analysis of the FPAR inversions of different vegetation indices showed that the inversion accuracies of the red-edge vegetation indices NDVIred-edge and SRred-edge were higher than those of the other vegetation indices, which confirmed that the vegetation indices involving red-edge information can more effectively retrieve FPAR and aboveground biomass of crops.
Editorial of Special Issue “Machine and Deep Learning for Earth Observation Data Analysis”
Authors: Syrris Vasileios , Loekken Sveinung
Published: July 2021

Earth observation and remote sensing technologies provide ample and comprehensive information regarding the dynamics and complexity of the Earth system [...]
Inversion of Geothermal Heat Flux under the Ice Sheet of Princess Elizabeth Land, East Antarctica
Authors: Li Lin , Tang Xueyuan , Guo Jingxue , Cui Xiangbin , Xiao Enzhao , Latif Khalid , Sun Bo , Zhang Qiao , Shi Xiaosong
Published: July 2021

Antarctic geothermal heat flux is a basic input variable for ice sheet dynamics simulation. It greatly affects the temperature and mechanical properties at the bottom of the ice sheet, influencing sliding, melting, and internal deformation. Due to the fact that the Antarctica is covered by a thick ice sheet, direct measurements of heat flux are very limited. This study was carried out to estimate the regional heat flux in the Antarctic continent through geophysical inversion. Princess Elizabeth Land, East Antarctica is one of the areas in which we have a weak understanding of geothermal heat flux. Through the latest airborne geomagnetic data, we inverted the Curie depth, obtaining the heat flux of bedrock based on the one-dimensional steady-state heat conduction equation. The results indicated that the Curie depth of the Princess Elizabeth Land is shallower than previously estimated, and the heat flux is consequently higher. Thus, the contribution of subglacial heat flux to the melting at the bottom of the ice sheet is likely greater than previously expected in this region. It further provides research clues for the formation of the developed subglacial water system in Princess Elizabeth Land.