bluuebunny/crossref_metadata_2025 huggingface dataset:
A new type II CHH neuropeptide involves ovarian development in the peppermint shrimp, Lysmata vittata
Authors: Lan Huiling , Liu Fang , Lu Li , Liu An , Ye Haihui
Published: August 2024

Type II crustacean hyperglycemic hormone (CHH) neuropeptides play diverse roles in crustaceans. In the hermaphrodite shrimp Lysmata vittata, two transcripts of type II CHHs (molt-inhibiting hormone/gonad-inhibiting hormone, MIH/GIH1 and MIH/GIH2) were identified by transcriptome sequencing, and MIH/GIH1 was later named Lvit-GIH1 for its inhibitory effect on ovarian development. Based on the high similarity of MIH/GIH2 to Lvit-GIH1, we named tentatively MIH/GIH2 as Lvit-GIH2 and explored the role of Lvit-GIH2 in ovarian development. The open reading frame (ORF) of Lvit-GIH2 was 333 bp in length, encoding a precursor consisted of a 32-aa signal peptide and a 78-aa mature peptide, which shared high sequence similarity with the type II subfamily peptides in crustaceans. Notably, Lvit-GIH2 was widely expressed in multiple tissues. The qRT-PCR findings indicated a rising trend in the expression of Lvit-GIH2 from the male phase to the euhermaphrodite phase. Both RNA interference and addition of GIH2 recombinant proteins (rGIH2) experiments showed that Lvit-GIH2 suppressed Lvit-Vg expression in hepatopancreas and Lvit-VgR expression in ovary. To further investigate the role of Lvit-GIH2 in ovarian development, the RNA-sequence analysis was performed to examine the changes in ovary after addition of rGIH2. The results showed that the pathways (Cysteine and methionine metabolism, Apoptosis-multiple species, etc.) and the genes (17bHSD8, IGFR, CHH, etc.) related to ovarian development were negatively regulated by rGIH2. In brief, Lvit-GIH2 might inhibit the ovarian development in L. vittata.
Perceived stress and coping strategies among nursing students towards rejoining college after covid-19 pandemic
Authors: Koirala Sarmila , Thapa Rupa Devi , Bhandari Sagun , Rijal Alisha , Thakuri Bhawani Shahi , Gauro Pooja
Published: August 2024

The COVID-19 pandemic has had a significant impact on nursingeducation, with many programs abruptly discontinuing clinical practice. This has raised concerns among nursing students about their clinicalskill development. Because of this, the researcher was motivated to learn how nursing students perceive stress and develop coping mechanisms for returning to nursing colleges following the COVID-19 pandemic. This study aimed to assess perceived stress and coping strategies among nursing students toward rejoining college after COVID -19 pandemic.Ananalyticalcross-sectionalstudywas conducted using proportionatestratified simple random sampling technique among 317 nursing students of bachelor level at all nursing colleges affiliated to Purbanchal University in Kathmandu Valley. Data was collected usingaself-administeredquestionnaire that included the Perceived Stress Scale (PSS) and the Brief COPEScale.The majority of respondents (71.3%) reported moderate stress levels, with 28.7%reportinghighstresslevels.Half of the respondents (51.7%) had low coping levels, while the other half (48.3%) had high coping levels. There was a significant association between the level of coping and mother’s occupation (p = 0.003). The Pearson correlation between perceived stress and coping strategies was moderately positive (r = 0.256, p = 0.001).Nursing college administration and instructors can play a role in reducing student stress and promoting coping strategies by maintaining a safecollege environment for students rejoining college after the COVID-19 pandemic.
Bedsharing among breastfeeding physicians: Results of a nationwide survey
Authors: Louis-Jacques Adetola F. , Bartick Melissa , Awomolo Adeola , Zhang Jiaqi , Feldman-Winter Lori , Leonard Stephanie A. , Meek Joan , Mitchell Katrina B. , Crowe Susan
Published: August 2024

Introduction

Bedsharing is common but advised against by the American Academy of Pediatrics. It is unknown if breastfeeding physicians bedshare more or less than the general population.

Objectives

To determine the prevalence of bedsharing among physicians, their reasons for bedsharing or not, and whether bedsharing was associated with a longer duration of breastfeeding.

Methods

An online survey was adapted from surveys administered by the Centers for Disease Control and Prevention. The survey was administered to physicians and medical students who birthed children from October 2020 through August 2021. Respondents were asked to report on a singleton birth, and questions centered around sleep practices and breastfeeding. Survival analysis was used to examine the association between bedsharing and breastfeeding duration.

Results

Of 546 respondents with bedsharing data, 68% reported some history of bedsharing, and 77% were in specialties that involved caring for pregnant people and/or infants. Those who bedshared breastfed an average of four months longer than those who never bedshared (18.08 versus 14.08 months p<0.001). The adjusted risk of breastfeeding cessation was markedly lower for those who bedshared compared to those who did not (Hazard Ratio 0.57, 95% Confidence Interval 0.45, 0.71). The primary reason for bedsharing was to breastfeed (73%); the primary reason for not bedsharing was safety concerns (92%). Among those who bedshared (n = 373), 52% did not inform their child’s healthcare provider.

Conclusions

Bedsharing is common among our sample of mainly breastfeeding physicians, including those who care for pregnant people and/or infants. It is also associated with a longer duration of breastfeeding, which has implications for population health. Practicing bedsharing implies cognitive dissidence and may affect how physicians counsel about bedsharing. Additionally, lack of disclosure of bedsharing practices has implications for practical guidance about having open non-judgmental conversations and may be a missed opportunity to counsel on bedsharing safety.
METTL16 participates in haemoglobin H disease through m6A modification
Authors: Liao Yuping , Zhang Feng , Yang Fang , Huang Shijin , Su Sha , Tan Xuemei , Zhong Linlin , Deng Lingjie , Pang Lihong
Published: August 2024

Background

Haemoglobin H (HbH) disease is caused by a disorder of α-globin synthesis, and it results in a wide range of clinical symptoms. M6A methylation modification may be one of the mechanisms of heterogeneity. Therefore, this article explored the role of methyltransferase like 16 (METTL16) in HbH disease.

Method

The results of epigenetic transcriptome microarray were analysed and verified through bioinformatic methods and qRT-PCR, respectively. The overexpression or knock down of METTL16 in K562 cells was examined to determine its role in reactive oxygen species (ROS), cell cycle processes or iron overload. YTH domain family protein 3 (YTHDF3) was knocked down in K562 cells and K562 cells overexpressing METTL16 via siRNA to investigate its function. In addition, haemoglobin expression was detected through benzidine staining. qRT-PCR, WB, methylated RNA Immunoprecipitation (MeRIP) and (RNA Immunoprecipitation) RIP experiments were conducted to explore the mechanism of intermolecular interaction.

Results

METTL16, YTHDF3 and solute carrier family 5 member 3 (SLC5A3) mRNA and the methylation level of SLC5A3 mRNA were downregulated in HbH patients. Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) mRNA expression was negatively correlated with HGB content among patients with HbH-CS disease. Overexpression of METTL16 increased ROS and intracellular iron contents in K562 cells, changed the K562 cell cycle, reduced hemin-induced haemoglobin synthesis, increased the expressions of SLC5A3 and HBG and increased SLC5A3 mRNA methylation levels. Knockdown of METTL16 reduced ROS and intracellular iron contents in K562 cells. Hemin treatment of K562 cells for more than 14 days reduced the protein expressions of METTL16 and SLC5A3 and SLC5A3 mRNA methylation levels. Knockdown of YTHDF3 rescued the intracellular iron content changes induced by the overexpression of METTL16. The RIP experiment revealed that SLC5A3 mRNA can be enriched by METTL16 antibody.

Conclusion

METTL16 may affect the expression of SLC5A3 by changing its m6A modification level and regulating ROS synthesis, intracellular iron and cycle of red blood cells. Moreover, METTL16 possibly affects the expression of haemoglobin through IGF2BP3, which regulates the clinical phenotype of HbH disease.
Large changes in detected selection signatures after a selection limit in mice bred for voluntary wheel-running behavior
Authors: Hillis David A. , Yadgary Liran , Weinstock George M. , de Villena Fernando Pardo-Manuel , Pomp Daniel , Garland Theodore
Published: August 2024

In various organisms, sequencing of selectively bred lines at apparent selection limits has demonstrated that genetic variation can remain at many loci, implying that evolution at the genetic level may continue even if the population mean phenotype remains constant. We compared selection signatures at generations 22 and 61 of the “High Runner” mouse experiment, which includes 4 replicate lines bred for voluntary wheel-running behavior (HR) and 4 non-selected control (C) lines. Previously, we reported multiple regions of differentiation between the HR and C lines, based on whole-genome sequence data for 10 mice from each line at generation 61, which was >31 generations after selection limits had been reached in all HR lines. Here, we analyzed pooled sequencing data from ~20 mice for each of the 8 lines at generation 22, around when HR lines were reaching limits. Differentiation analyses of allele frequencies at ~4.4 million SNP loci used the regularized T-test and detected 258 differentiated regions with FDR = 0.01. Comparable analyses involving pooling generation 61 individual mouse genotypes into allele frequencies by line produced only 11 such regions, with almost no overlap among the largest and most statistically significant peaks between the two generations. These results implicate a sort of “genetic churn” that continues at loci relevant for running. Simulations indicate that loss of statistical power due to random genetic drift and sampling error are insufficient to explain the differences in selection signatures. The 13 differentiated regions at generation 22 with strict culling measures include 79 genes related to a wide variety of functions. Gene ontology identified pathways related to olfaction and vomeronasal pathways as being overrepresented, consistent with generation 61 analyses, despite those specific regions differing between generations. Genes Dspp and Rbm24 are also identified as potentially explaining known bone and skeletal muscle differences, respectively, between the linetypes.
Investigating the efficacy of transcranial direct current stimulation on chronic pain management in endometriosis patients: A randomized controlled trial protocol
Authors: Camila de Lima Alves da Silva Tatiana , da Silva Dantas Hégila , Eduarda Macedo Luiza , Duarte Martins Talita , Silva-Filho Edson , Pegado Rodrigo , McLean Linda , Thereza Albuquerque Barbosa Cabral Micussi Maria
Published: August 2024

Introduction

Similar to chronic pain conditions, individuals with endometriosis can be affected by central sensitization syndrome (CSS), which is characterized by a loss of analgesia and central amplification of pain. Transcranial direct current stimulation (tDCS) has shown potential as an effective intervention to improve pain generated by other chronic pain conditions impacted by CSS, such as fibromyalgia and chronic pelvic issues. This study aims to evaluate the effectiveness of tDCS on pain, fatigue, and quality of life among patients affected by endometriosis.

Methods

This is a single-center, parallel, double-blinded, randomized, controlled clinical trial protocol study. We aim to recruit 40 participants affected by endometriosis (active group, n = 20; sham group, n = 20). Anodal tDCS will be delivered at an intensity of 2mA, applied over the primary motor cortex for 20 minutes per day for 10 consecutive days. There will be four assessment times: 1 week before beginning the intervention; on the 10thday following the last tDCS session; and 1 and 2 months after the last tDCS session. Pain evaluated by the algometry will be the primary outcome. Pain intensity, quality of life, fatigue, and global perception of change will be the secondary outcomes. We will calculate the effects of the active versus sham stimulation on primary and secondary outcomes by using generalized estimated equations or mixed model analysis. The effect size calculation will represent the effect measure. We expect that only the active group show reductions in pain, fatigue, and quality of life. The results of this trial will produce an important first step in providing evidence on the effectiveness of neuromodulation for the management of pain and will provide data to support new studies on tDCS.

Registration

Brazilian Clinical Trials Registry (RBR-4q69573).
The impact of lutein-loaded poly(lactic-co-glycolic acid) nanoparticles following topical application: An in vitro and in vivo study
Authors: Carter Renee T. , Swetledge Sean , Navarro Sara , Liu Chin-C. , Ineck Nikole , Lewin Andrew C. , Donnarumma Fabrizio , Bodoki Ede , Stout Rhett W. , Astete Carlos , Jung Jangwook P. , Sabliov Cristina M.
Published: August 2024

Antioxidant therapies are of interest in the prevention and management of ocular disorders such as cataracts. Although an active area of interest, topical therapy with antioxidants for the treatment of cataracts is complicated by multiple ocular anatomical barriers, product stability, and solubility. Entrapment and delivery of antioxidants with poly(lactic-co-glycolic acid) nanoparticles is a possible solution to these challenges, however, little is known regarding their effects in vitro or in vivo. Our first aim was to investigate the impact of blank and lutein loaded PLGA nanoparticles on viability and development of reactive oxygen species in lens epithelial cells in vitro. Photo-oxidative stress was induced by ultraviolet light exposure with cell viability and reactive oxygen species monitored. Next, an in vivo, selenite model was utilized to induce cataract formation in rodents. Eyes were treated topically with both free lutein and lutein loaded nanoparticles (LNP) at varying concentrations. Eyes were monitored for the development of anterior segment changes and cataract formation. The ability of nanodelivered lutein to reach the anterior segment of the eye was evaluated by liquid chromatography coupled to mass spectrometry of aqueous humor samples and liquid chromatography coupled to tandem mass spectrometry (targeted LC-MS/MS) of lenses. LNP had a minimal impact on the viability of lens epithelial cells during the short exposure timeframe (24 h) and at concentrations < 0.2 μg LNP/μl. A significant reduction in the development of reactive oxygen species was also noted. Animals treated with LNPs at an equivalent lutein concentration of 1,278 μg /mL showed the greatest reduction in cataract scores. Lutein delivery to the anterior segment was confirmed through evaluation of aqueous humor and lens sample evaluation. Topical treatment was not associated with the development of secondary keratitis or anterior uveitis when applied once daily for one week. LNPs may be an effective in the treatment of cataracts.
Normatively irrelevant disgust interferes with decision under uncertainty: Insights from the Iowa gambling task
Authors: Priolo Giulia , D’Alessandro Marco , Bizzego Andrea , Franchin Laura , Bonini Nicolao
Published: August 2024

This study investigates whether a not informative, irrelevant emotional reaction of disgust interferes with decision-making under uncertainty. We manipulate the Iowa Gambling Task (IGT) by associating a disgust-eliciting image with selections from Disadvantageous/Bad decks (Congruent condition) or Advantageous/Good decks (Incongruent condition). A Control condition without manipulations is also included. Results indicate an increased probability of selecting from a Good deck as the task unfolds in all conditions. However, this effect is modulated by the experimental manipulation. Specifically, we detect a detrimental effect (i.e., a significant decrease in the intercept) of the disgust-eliciting image in Incongruent condition (vs. Control), but this effect is limited to the early stages of the task (i.e., first twenty trials). No differences in performance trends are detected between Congruent and Control conditions. Anticipatory Skin Conductance Response, heart rate, and pupil dilation are also assessed as indexes of anticipatory autonomic activation following the Somatic Marker Hypothesis, but no effects are shown for the first two indexes in any of the conditions. Only a decreasing trend is detected for pupil dilation as the task unfolds in Control and Incongruent conditions. Results are discussed in line with the “risk as feelings” framework, the Somatic Marker Hypothesis, and IGT literature.
The label-feedback effect is influenced by target category in visual search
Authors: Frugarello Paolo , Rusconi Elena , Job Remo
Published: August 2024

The label-feedback hypothesis states that language can modulate visual processing. In particular, hearing or reading aloud target names (labels) speeds up performance in visual search tasks by facilitating target detection and such advantage is often measured against a condition where the target name is shown visually (i.e. via the same modality as the search task). The current study conceptually complements and expands previous investigations. The effect of a multimodal label presentation (i.e., an audio+visual, AV, priming label) in a visual search task is compared to that of a multimodal (i.e. white noise+visual, NV, label) and two unimodal (i.e. audio, A, label or visual, V, label) control conditions. The name of a category (i.e. a label at the superordinate level) is used as a cue, instead of the more commonly used target name (a basic level label), with targets belonging to one of three categories: garments, improper weapons, and proper weapons. These categories vary for their structure, improper weapons being an ad hoc category (i.e. context-dependent), unlike proper weapons and garments. The preregistered analysis shows an overall facilitation of visual search performance in the AV condition compared to the NV condition, confirming that the label-feedback effect may not be explained away by the effects of multimodal stimulation only and that it extends to superordinate labels. Moreover, exploratory analyses show that such facilitation is driven by the garments and proper weapons categories, rather than improper weapons. Thus, the superordinate label-feedback effect is modulated by the structural properties of a category. These findings are consistent with the idea that the AV condition prompts an "up-regulation" of the label, a requirement for enhancing the label’s beneficial effects, but not when the label refers to an ad hoc category. They also highlight the peculiar status of the category of improper weapons and set it apart from that of proper weapons.
Shifts in biodiversity and physical structure of seagrass beds across 5 decades at Carriacou, Grenadines
Authors: Patriquin David , Scheibling Robert E. , Filbee-Dexter Karen
Published: August 2024

Caribbean seagrass beds are facing increasing anthropogenic stress, yet comprehensive ground-level monitoring programs that capture the structure of seagrass communities before the 1980s are rare. We measured the distribution of seagrass beds and species composition and abundance of seagrass and associated macroalgae and macroinvertebrates in 3 years over a 47-year period (1969, 1994, 2016) at Carriacou, Granada, an area not heavily impacted by local human activity. Seagrass cover and physical parameters of fringing beds were measured in transects at high (HWE) and low wave energy (LWE) sites; frequency of occurrence of all species, and biomass and morphology of seagrasses, were measured at 100 m2 stations around the island. Losses in nearshore seagrass cover occurred at HWE but not LWE sites between 1969 and 2016 and were associated with increases in the seagrass-free inshore zone (SFI) and erosional scarps within beds. Total biomass did not vary across years although there were progressive changes in seagrass composition: a decline in the dominant Thalassia testudinum and concomitant increase in Syringodium filiforme, and establishment of invasive Halophila stipulacea in 2016 at LWE sites. Species richness and diversity of the seagrass community were highest in 1994, when 94% of macroalgae (excluding Caulerpa) were most abundant, and sea urchins were least abundant, compared to 1969 and 2016. Multivariate statistical analyses showed differences in community composition across the 3 years that were consistent with trends in urchin abundance. Increases in SFI and scarp number in seagrass beds at HWE sites occurred mainly after 1994 and likely were related to increased wave forcing following degradation of offshore coral reefs between 1994 and 2016. Our observations suggest that landward migration of seagrass beds with rapidly rising sea level in future will not be realized in reef-protected seagrass beds at Carriacou barring reversal in the processes that have caused reef flattening.
Integrated maternity care: A concept analysis
Authors: Cellissen Evelien , van Zelm Ruben , Hendrix Marijke , Wildschut Hajo I. J. , Nieuwenhuijze Marianne
Published: August 2024

Introduction

Integrated maternity care is strongly promoted in the Netherlands. However, the term ‘integrated’ and its practical meaning is understood differently by professionals and policy makers. This lack of clarity is also visible in other countries and hinders implementation. In this study, we will examine how the concept of ‘integrated maternity care’ and its defining attributes are presented in the international literature.

Methods

This study aims to provide a definition and deeper understanding of the concept of integrated maternity care by conducting a concept analysis using Morse’s method. We performed a systematic search using Embase and Ebscohost (CINAHL, PsychINFO, SocINDEX, MEDLINE) including records that described integrated maternity care from on organizational perspective. Through a qualitative analysis of the selected research and non-research records, we identified defining attributes, boundaries, antecedents, and consequences of the concept. Subsequently, we constructed a definition of the concept based on the findings.

Results

We included 36 records on integrated maternity care in the period from 1978 to 2022. Our search included 21 research and 15 non-research records (e.g. guidelines and policy records). Only half of these had a definition of integrated maternity care. Over time, the definition became more specific. Our concept analysis resulted in three defining attributes of integrated maternity care: collaboration, organizing collaboration and woman-centeredness. We identified role clarity, a culture of collaboration, and clear and timely communication as antecedents of integrated maternity care. A number of consequences were found: continuity of care, improved outcomes, and efficiency. All consequences were described as expected effects of integrated maternity care and not based on evidence.

Conclusion

We propose the following definition: ‘Integrated maternity care is woman-centred care provided by (maternity) care professionals collaborating together within and across different levels of healthcare with a specific focus on organizing seamless care.’ Addressing the antecedents is important for the successful implementation of integrated maternity care.
Quantitative evaluation of science and technology financial policies based on the PMC-AE index model: A case study of China’s science and technology financial policies since the 13th five-year plan
Authors: Shen Hongyuan , Xiong Panyu , Yang Linfeng , Zhou Ling
Published: August 2024

The formulation of science and technology financial policies directly influences the direction of national economic development. Quantitative evaluation of these policies is an important method to reflect the consistency and strengths and weaknesses of policy interrelations. This paper analyzes 16 science and technology financial policy documents issued by the Chinese central government from 2016 to 2022, using text analysis and content analysis to extract keyword frequencies, and constructs 9 primary variables and 34 secondary variables. For the first time, a PMC-AE index model for science and technology financial policies is established, and a quantitative evaluation is conducted on 5 significant policy documents out of the 16. The results show that, from an overall analysis, Policy 1 and Policy 4 are at a good level, while the other three policies are at an excellent level. From the analysis of individual policy PMC-AE indexes, the rankings in descending order are: P2 > P5 > P3 > P4 > P1. Overall, the policies effectively meet the needs of China’s science and technology financial development, with P2, P3, and P5 being at an excellent level, P4 at a good level, and P1 at an acceptable level, mainly reflecting the need for improvement in aspects such as policy synchronization with the current stage, targeted entities, guiding fields, and policy content. It is recommended that Chinese government departments should focus on five aspects in policy formulation: building a talent system for science and technology finance, improving the quality of financial services, coordinating central and local financial policies, protecting intellectual property rights in science and technology finance, and strengthening financial supervision. This will be conducive to the effective implementation of science and technology financial policies.
Infant and young child feeding practices among mothers in the pilot Micronutrient Powder Initiative in four geographically and ethnically diverse districts in Ghana
Authors: Kyei-Arthur Frank , Aballo Jevaise , Mahama Abraham B. , Adu-Afarwuah Seth
Published: August 2024

In Ghana, breastfeeding and complementary feeding counselling have been used as a nutritional intervention to promote optimal Infant and Young Child Feeding (IYCF) and nutrition. This study examined IYCF practices in four geographically and ethnically diverse districts (Ho West, Tain, Talensi and Tolon). A qualitative study involving key informant interviews (KIIs) and focus group discussions (FGDs) was conducted between November and December 2019 among women who participated in a pilot micronutrient powder intervention for children 6–23 months of age. The KIIs and FGDs were audio-taped, transcribed verbatim, and analyzed thematically using NVivo 10. Three themes emerging from the KIIs and FGDs were: level of adherence to IYCF recommendations among mothers and caregivers; IYCF recommendations perceived as the hardest to follow; and perceived motivators, facilitators, and barriers to IYCF practices. Mothers in the four districts generally followed the eight IYCF recommendations. Mothers in the Tolon district demonstrated adherence to IYCF practices, often citing the need for early initiation of breastfeeding, timely introduction of complementary feeding, and feeding children aged 9–23 months 3 times daily in addition to breastfeeding. In contrast, mothers in other districts faced challenges that hindered adherence. Giving children 4 or more food groups and timely introduction of complementary feeding were perceived as the hardest practices to follow. The main facilitators of IYCF practices include midwives and frontline nurses teaching mothers how to breastfeed, and midwives ensuring mothers initiate breastfeeding immediately after delivery. The main barriers to IYCF practices identified were insufficient breastmilk; mothers-in-law giving water to children before six months; resumption of work; and lack of financial means. Mothers in the Ho West district reported more barriers to IYCF practices, followed by mothers in the Tain, Talensi, and Tolon districts. Health practitioners, stakeholders, and policymakers should design targeted interventions that address the contextual barriers to improve IYCF practices in the various districts.
Area under the inspiratory flow-volume curve (AIN): Proposed normative values
Authors: Ioachimescu Octavian C. , Stoller James K.
Published: August 2024

Rationale

Area under expiratory flow-volume curve (AEX) has been shown to be a valuable functional measurement in respiratory physiology. Area under inspiratory flow-volume loop (AIN) also shows promise in characterizing upper and/or lower airflow obstruction.

Objectives

we aimed here to develop normative reference values for AIN, able to ascertain deviations from normal.

Methods

We analyzed AIN in 4,980 spirometry tests recorded in non-smoking, healthy individuals in the Pulmonary Function Testing Laboratory.

Results

The mean (95% confidence interval, CI), standard deviation and median (25th-75th interquartile range) AIN were 16.05 (15.79–16.31), 9.08 and 14.72 (9.12–21.42) L2·sec-1, respectively. The mean (95% CI) and standard deviation of the best-trial measurements for square root of AIN (Sqrt AIN) were 3.84 (3.81–3.87) and 1.14; 4.15 (4.12–4.18) and 1.03 in men, and 2.68 (2.63–2.72) and 0.72 L·sec-1/2 in women. The mean (standard deviation) of pre- and post-bronchodilator Sqrt AIN were 3.71 (1.17) and 3.81 (1.19) L·sec-1/2, respectively.

The mean (95% CI), standard deviation and lowest 5th percentile (lower limit of normal, LLN) of Sqrt AIN/Sqrt AEX (%) were 101.3 (100.82–101.88), 18.7, and 71.8%; stratified by gender, it was 102.2 (101.6–102.8), 18.6, and 72.8% in men, and 98 (96.9–99.2), 18.8, and 68.6% in women, respectively.

Conclusions

The availability of area under the inspiratory flow-volume curve (AIN) and the derived indices offers a promising opportunity to assess upper airway disease (e.g., involvement of larynx, trachea or major bronchi), especially because some of these measurements appear to be independent of age, race, height, and weight.
Behaviour change communication influences on food consumption behaviours and the demand for diverse nutritious foods in the Makoni District, Zimbabwe
Authors: Takawira Delilah , Zuma Mthokozisi Kwazi , Mbhenyane Xikombiso Gertrude
Published: August 2024

This study evaluated the effectiveness of nutrition behavioural change communication interventions and food consumption behaviours and demand for nutritious foods in Makoni district, Zimbabwe. The study employed an observational and cross-sectional design using mixed—methods. The population was smallholder farmers’ households with children six to twenty-four months old. Secondary data was obtained from the main intervention reports. The total sample size of this project comprised of five personnel participating in the implementation of the intervention for key informant interviews; forty participants for the in-depth interviews; and a total of 81 participants for eight focus group discussions. Participants indicated that the Livelihoods and Food Security Programme intervention successfully increased their nutrition knowledge, enhanced their ability to diversify crop production, and improved their access to varied foods, including some new crops. Local markets had little influence on the demand for nutritious foods by the intervention population. The interventions were effective in stimulating demand for diverse and nutritious foods in Makoni District.
AmiR-P3: An AI-based microRNA prediction pipeline in plants
Authors: Ataei Sobhan , Ahmadi Jafar , Marashi Sayed-Amir , Abolhasani Ilia
Published: August 2024

Background

MicroRNAs (miRNAs) are small noncoding RNAs that play important post-transcriptional regulatory roles in animals and plants. Despite the importance of plant miRNAs, the inherent complexity of miRNA biogenesis in plants hampers the application of standard miRNA prediction tools, which are often optimized for animal sequences. Therefore, computational approaches to predict putative miRNAs (merely) from genomic sequences, regardless of their expression levels or tissue specificity, are of great interest.

Results

Here, we present AmiR-P3, a novel ab initio plant miRNA prediction pipeline that leverages the strengths of various utilities for its key computational steps. Users can readily adjust the prediction criteria based on the state-of-the-art biological knowledge of plant miRNA properties. The pipeline starts with finding the potential homologs of the known plant miRNAs in the input sequence(s) and ensures that they do not overlap with protein-coding regions. Then, by computing the secondary structure of the presumed RNA sequence based on the minimum free energy, a deep learning classification model is employed to predict potential pre-miRNA structures. Finally, a set of criteria is used to select the most likely miRNAs from the set of predicted miRNAs. We show that our method yields acceptable predictions in a variety of plant species.

Conclusion

AmiR-P3 does not (necessarily) require sequencing reads and/or assembled reference genomes, enabling it to identify conserved and novel putative miRNAs from any genomic or transcriptomic sequence. Therefore, AmiR-P3 is suitable for miRNA prediction even in less-studied plants, as it does not require any prior knowledge of the miRNA repertoire of the organism. AmiR-P3 is provided as a docker container, which is a portable and self-contained software package that can be readily installed and run on any platform and is freely available for non-commercial use from: https://hub.docker.com/r/micrornaproject/amir-p3
Potentiation of anti-angiogenic eNOS-siRNA transfection by ultrasound-mediated microbubble destruction in ex vivo rat aortic rings
Authors: Villa-Martínez Elisa , Rios Amelia , Gutiérrez-Vidal Roxana , Escalante Bruno
Published: August 2024

Nitric oxide (NO) regulates vascular homeostasis and plays a key role in revascularization and angiogenesis. The endothelial nitric oxide synthase (eNOS) enzyme catalyzes NO production in endothelial cells. Overexpression of the eNOS gene has been implicated in pathologies with dysfunctional angiogenic processes, such as cancer. Therefore, modulating eNOS gene expression using small interfering RNAs (siRNAs) represents a viable strategy for antitumor therapy. siRNAs are highly specific to the target gene, thus reducing off-target effects. Given the widespread distribution of endothelium and the crucial physiological role of eNOS, localized delivery of nucleic acid to the affected area is essential. Therefore, the development of an efficient eNOS-siRNA delivery carrier capable of controlled release is imperative for targeting specific vascular regions, particularly those associated with tumor vascular growth. Thus, this study aims to utilize ultrasound-mediated microbubble destruction (UMMD) technology with cationic microbubbles loaded with eNOS-siRNA to enhance transfection efficiency and improve siRNA delivery, thereby preventing sprouting angiogenesis. The efficiency of eNOS-siRNA transfection facilitated by UMMD was assessed using bEnd.3 cells. Synthesis of nitric oxide and eNOS protein expression were also evaluated. The silencing of eNOS gene in a model of angiogenesis was assayed using the rat aortic ring assay. The results showed that from 6 to 24 h, the transfection of fluorescent siRNA with UMMD was twice as high as that of lipofection. Moreover, transfection of eNOS-siRNA with UMMD enhanced the knockdown level (65.40 ± 4.50%) compared to lipofectamine (40 ± 1.70%). Silencing of eNOS gene with UMMD required less amount of eNOS-siRNA (42 ng) to decrease the level of eNOS protein expression (52.30 ± 0.08%) to the same extent as 79 ng of eNOS-siRNA using lipofectamine (56.30 ± 0.10%). NO production assisted by UMMD was reduced by 81% compared to 67% reduction transfecting with lipofectamine. This diminished NO production led to higher attenuation of aortic ring outgrowth. Three-fold reduction compared to lipofectamine transfection. In conclusion, we propose the combination of eNOS-siRNA and UMMD as an efficient, safe, non-viral nucleic acid transfection strategy for inhibition of tumor progression.
A single-center retrospective study on the clinical features of thyrotoxic periodic paralysis
Authors: Yamada Kota , Tanabe Akiyo , Hashimoto Makiko , Ohsugi Mitsuru , Ueki Kohjiro , Kajio Hiroshi
Published: August 2024

Purpose

Thyrotoxic periodic paralysis (TPP) is characterized by muscle paralysis and significant intracellular potassium movement resulting in hypokalemia. Since TPP is a rare condition, only a few studies have explicated the clinical characteristics of patients with this disease. This study aimed to elucidate the clinical characteristics of patients with TPP by comparing them with those with thyrotoxicosis without paralysis (non-TPP) and sporadic periodic paralysis (SPP).

Methods

This was a single-center retrospective cohort study. Clinical data of patients with hyperthyroidism (n = 62) or periodic paralysis (n = 92) who were emergently admitted to our hospital was extracted from the electronic medical records and analyzed.

Results

All patients in the TPP group (15 males and 2 females) had Graves’ disease, with 14 being newly diagnosed. The average serum potassium level on admission was 2.3±0.75 mEq/L. No significant correlation was observed among serum potassium level, amount of potassium required for normalization, and thyroid hormone levels. The TPP group showed significantly younger age, higher male ratio and body mass index (BMI), and lower serum potassium and phosphorus levels than the non-TPP group, which comprised 36 patients with Graves’ disease. No significant differences were observed between the TPP and SPP (n = 11) groups in terms of age, sex, BMI, serum electrolyte levels, potassium requirement for normalization, and recovery time.

Main conclusions

Considering that most patients with TPP have undiagnosed Graves’ disease, distinguishing TPP from SPP based on clinical information and course alone is difficult in emergency settings. Therefore, for early detection and launch of specific treatment of Graves’ disease, screening for thyroid hormone and anti-thyroid stimulating hormone receptor antibody levels is necessary when treating patients with periodic paralysis.
Exploring user acceptance of online virtual reality exhibition technologies: A case study of Liangzhu Museum
Authors: Li Jia , Lv Chan
Published: August 2024

Museums increasingly rely on cutting-edge digital technologies to attract visitors. Understanding the intricate factors influencing user acceptance of these technologies is, however, crucial for their effective use. This study therefore proposes a model, grounded in the technology acceptance model, to investigate user acceptance of online virtual reality (VR) museum exhibitions. Leveraging the online VR exhibition at Liangzhu Museum as a case study, data were collected from 313 participants and analyzed using partial least squares structural equation modeling (PLS-SEM) with Smart PLS. Semi-structured interviews with 15 individuals were conducted to complement the quantitative findings. The results reveal that factors such as interactivity, immersion, and presence positively influenced users’ intrinsic technological beliefs (perceived ease of use, perceived enjoyment, and perceived usefulness), ultimately affecting their willingness to use and intention to visit on-site. Notably, immersion had a direct positive effect on perceived usefulness. There is a pressing need to leverage digital and web technologies to cater to the increasingly complex and diverse needs of online visitors, and emphasizing navigational performance in online VR exhibitions is also paramount for enhancing the overall user experience.
Effects of moderate doses of ionizing radiation on experimental abdominal aortic aneurysm
Authors: Riazi Goran , Brizais Chloe , Garali Imene , Al-rifai Rida , Quelquejay Helene , Monceau Virginie , Vares Guillaume , Ould-Boukhitine Lea , Aubeleau Damien , Gilain Florian , Gloaguen Celine , Dos Santos Morgane , Ait-Oufella Hafid , Ebrahimian Teni
Published: August 2024

Background

Exposure to ionizing radiation has been linked to cardiovascular diseases. However, the impact of moderate doses of radiation on abdominal aortic aneurysm (AAA) remains unknown.

Methods

Angiotensin II-infusedApoe-/-mice were irradiated (acute, 1 Gray) either 3 days before (Day-3) or 1 day after (Day+1) pomp implantation. Isolated primary aortic vascular smooth muscle cells (VSMCs) were irradiated (acute 1 Gray) for mechanistic studies and functional testing in vitro.

Results

Day-3 and Day+1 irradiation resulted in a significant reduction in aorta dilation (Control: 1.39+/-0.12; Day-3: 1.12+/-0.11; Day+1: 1.15+/-0.08 mm, P<0.001) and AAA incidence (Control: 81.0%; Day-3: 33.3%, Day+1: 53.3%) compared to the non-irradiated group. Day-3 and Day+1 irradiation led to an increase in collagen content in the adventitia (Thickness control: 23.64+/-2.9; Day-3: 54.39+/-15.5; Day+1 37.55+/-10.8 mm, P = 0.006). However, the underlying protective mechanisms were different between Day-3 and Day+1 groups. Irradiation before Angiotensin II (AngII) infusion mainly modulated vascular smooth muscle cell (VSMC) phenotype with a decrease in contractile profile and enhanced proliferative and migratory activity. Irradiation after AngII infusion led to an increase in macrophage content with a local anti-inflammatory phenotype characterized by the upregulation of M2-like gene and IL-10 expression.

Conclusion

Moderate doses of ionizing radiation mitigate AAA either through VSCM phenotype or inflammation modulation, depending on the time of irradiation.
Patient satisfaction with the management of refractory and unexplained chronic cough in Canada: Results from a national survey
Authors: Khan Sana , Brister Danica , Abraham Ted , Laventure Samuel , Sahakian Sevag , Juliá Berta , Satia Imran
Published: August 2024

Background

Chronic cough (persisting for ≥8 weeks) is a common disorder affecting approximately 5 to 10% of adults worldwide that is sometimes refractory to treatment (refractory chronic cough [RCC]) or has no identifiable cause (unexplained chronic cough [UCC]). There is minimal information on the patient’s experience of RCC/UCC in Canada. The aim of this study was to evaluate the patient journey and perceptions related to RCC/UCC management in Canada.

Methods

Our exploratory study included Canadians in the Leger Opinion Panel and focused on individuals with RCC or UCC. Key entry criteria were: age ≥18 years, cough on most days for ≥8 weeks, no smoking within 1 year, no serious respiratory disease or lung cancer, and not taking angiotensin-converting enzyme inhibitors. Individuals who met entry criteria were invited to complete an approximately 30-minute online survey with questions on demographic characteristics, healthcare professional (HCP) interactions, diagnosis of underlying conditions, current treatments, and satisfaction with HCPs and chronic cough therapies.

Results

A total of 49,076 individuals completed the chronic cough screening questionnaire (July 30, 2021 to September 1, 2021): 1,620 (3.3%) met entry criteria for RCC or UCC, and 1,046 (2.1%) completed the online survey (mean age of 45 years, 61% female). Most respondents (58%) reported their chronic cough was managed by a general practitioner (GP). Forty-four percent of respondents did not have a diagnosis of an underlying condition for their cough. Breathing tests (39%) and chest imaging (34%) were the most common diagnostic tests. Cough suppressants (18%) were the most frequent current treatment. Respondents were moderately satisfied with their HCPs, but more than half considered their treatment ineffective and 34% had considered no longer seeking medical attention because of a lack of treatment success.

Conclusions

Individuals with RCC/UCC in Canada are largely unsatisfied with the effectiveness of treatment. Additional HCP education and new treatment options are needed to improve patient satisfaction.
Satyajit Ray: The Man Who Knew Too Much, Barun Chanda (2022)
Authors: Pal Shyam Sundar , Ghoshal Ananya
Published: August 2024

Review of: Satyajit Ray: The Man Who Knew Too Much, Barun Chanda (2022)

New Delhi: Om Books International, 347 pp.,

ISBN 978-9-39283-465-3, p/bk, $11.99
Ingenieros geógrafos en la vida política y académica del México decimonónico. Las generaciones olvidadas
Authors: Moncada Maya José Omar
Published: August 2024

En un reciente artículo se dio a conocer a los personajes que conformaron a la que hemos denominado “Primera generación” de ingenieros geógrafos. Ahí se destacó el importante rol que desempeñaron en la docencia, en las actividades científicas y en su actuar como funcionarios públicos en gobiernos de distintas tendencias políticas durante la segunda mitad del siglo XIX. Los tres personajes revisados tuvieron un gran reconocimiento por la comunidad científica y, tal vez, sobresalieron tanto que las dos generaciones que les siguieron no han tenido el reconocimiento que se merecen, pues igualmente destacaron en la actividad docente, en las labores académicas y ocupando destacados cargos públicos. Por ello, en este artículo se pretende dar a conocer a estos dos grupos de ingenieros geógrafos, formados ya en la Escuela Nacional de Ingenieros. Estas nuevas generaciones fueron producto del Porfiriato –lo que tal vez también influye en el poco reconocimiento que se les da al identificarlos con el periodo en que trabajaron–, toda vez que debieron pasar casi 25 años después de la obtención del título por los individuos de la primera generación, y los últimos cuatro ya son producto del siglo XX.
Vulnerabilidad y riesgo climático. Los retos de la Ciudad de México hacia el primer tercio del siglo XXI
Authors: Graizbord Boris , González Granillo José Luis , Lópes Ibarra Omar
Published: August 2024

. El presente artículo tiene como objetivo aportar diversas evidencias en torno a las condiciones de vulnerabilidad por cuestiones climáticas que presenta actualmente la población de la Ciudad de México, en especial, aquellas relacionadas con las islas de calor y otros eventos climáticos de carácter hidrometeorológico. Si bien la política local se ha enfocado hacia dicha urbe como una ciudad resiliente, a la fecha las acciones emprendidas por los gobiernos locales se encuentran en una fase de desarrollo temprano. Desde finales del siglo pasado, las acciones en materia de medio ambiente se centraban en combatir principalmente la contaminación atmosférica, en la actualidad las estrategias y programas de medio ambiente abarcan más ámbitos de acción y han considerado el cambio climático como eje transversal, dado que sus impactos trascienden los ámbitos económico, social y ambiental.
Fernández Christlieb, F. (2023). Hacer Geografía. Un razonamiento histórico para el mundo que viene
Authors: De Ita Rubio María Lourdes Guadalupe
Published: August 2024

Fernández Christlieb, F. (2023). Hacer Geografía. Un razonamiento histórico para el mundo que viene. México: Instituto de Geografía, UNAM 186 pp. ISBN 978-607-30-8097-2
Exposición temporal: Virreinato entre mares
Authors: Martínez Muñoz María Fernanda , Estrada López Fernando Fady
Published: August 2024

Exposición temporal: Virreinato entre mares Museo de El Carmen Sala de Arcos, planta baja Av. Revolución 4 y 6  San Ángel, alcaldía Álvaro Obregón, 01000 Ciudad de México Del 18 de junio al 22 de septiembre de 2024
Ultrasonographic differential diagnosis of medial elbow pain
Authors: Cho Min Jeong , Chai Jee Won , Kim Dong Hyun , Kim Hyo Jin , Seo Jiwoon
Published: August 2024

Medial elbow pain is a common musculoskeletal problem among individuals engaging in repetitive activities. Medial epicondylitis is the predominant cause of this pain. However, other potential causes must be considered as part of the differential diagnosis. This article discusses several etiologies of medial elbow pain, including medial epicondylitis, ulnar neuropathy, snapping triceps syndrome, ulnar collateral ligament injury, medial antebrachial cutaneous neuropathy, and diseases of the elbow joint, with an emphasis on ultrasound (US) findings. Awareness of possible diagnoses and their US features can assist radiologists in establishing a comprehensive diagnosis for medial elbow pain.