bluuebunny/crossref_metadata_2025 huggingface dataset:
Measurement of Consumer Satisfaction of Product Quality Using the Customer Satisfaction Index Method and Importance Performance Analysis
Authors: Wardana Acmad Bayu , Sumarmi Wiwik
Published: December 2022

Satisfaction of Consumer represent storey level satisfaction of consumer after what in processing in data processing. This research use glorious partner at CV case study of collection. This research aim to to know storey level satisfaction of consumer to quality of Glorious LIMITED PARTNER, CV Partner product of Collection with CSI method and which need to be improve repaired and developed any kind of according to IPA method. This Research is executed to Glorious LIMITED PARTNER, CV Partner cutomer client of Collection which have transacted more than one year. from this Research represent research of quantitative diskriptif with research device method. Amount of research sampel 100 Glorious LIMITED PARTNER, CV Partner cutomer client of Collection that is. Data analysis use Importance Performance Analysis ( IPA) And Customer Satifaction Index ( CSI). Result of research show attribute which need to be paid attention Glorious LIMITED PARTNER, CV Partner of Collection to fulfill satisfaction of its consumer is I kuadran that is Product specification ( 7), Price Product According to Quality ( 8), Product Company Have Positive Image In Company ( 18) Given By Company.
Japan-Uzbekistan Diplomatic Relations History
Authors: Zuhayriddin Tuychiyevich Bozorov
Published: December 2022

Over the years of independence, the Republic of Uzbekistan has established diplomatic relations with developed countries of the world and has taken a step towards mutually beneficial cooperation. In this article, Uzbekistan cooperates with Japan in political, socio-economic, cultural and humanitarian spheres
The Effect of Microelements on Tobacco Activity
Authors: Hashimov F. X  , Karimova Kh.B.
Published: December 2022

The article contains some information on the specific effects of molybdenum, cobalt and copper cellulose in the "Virginia" type "K-326" tobacco plant.
Ecological terms in “baburnama”
Authors: Z.Djurabaeva
Published: December 2022

The article is devoted to determine the tasks of ecolinguistics as a new branch of linguistics on the one hand, to throw light to the historical-etymological, lexical-semantic peculiarities of the lexical units used in “Baburnama” from the point of drew of ecology, as they make up a separate, system, consisting of definite subsystems.
New Reflections on the Occupation of Jizzakh by the Mongols
Authors: M.Kh. Pardaev  , G.I. Mo’minova
Published: December 2022

This article deals with the geographical location of the city of Djizzakh (Jizzak) during the Mongol invasion of  Maverannahr. And also, due to the lack of written sources of information about the conquest of the city of Djizzakh by the Mongol hordes, the authors set forth their points of view on this important and interesting issue.
Resourse-saving technology
Authors: N.A.Mamadjonova
Published: December 2022

In agriculture, special attention should be paid to reducing the cost of the product using innovative technologies that minimize the cost of fertilizers, fuels, and lubricants, mechanization services for the placement of fertile varieties in conditions of appropriate soil and climate based on  the integration of science, education and practice.
Evaluation of industry tendencies in Uzbekistan
Authors: Radjabov Bunyod Abduhalilovich
Published: December 2022

The article proposes a method for assessing trends in industrial development in Uzbekistan. The least-squares method of the regression model was used to estimate industry development trends. Development trends are assessed based on the index of change in the final and theoretical values of industrial production.
Analysis of Health Level Performance of Amil Zakat Institution Entities with the Indonesian Zakat & Development Report (IZDR) Approach
Authors: Rochmah Miftachur , Rahmawati Imelda Dian
Published: December 2022

The research used qualitative research method with an evaluation approach. The data collection was carried out in two ways, that are by direct interviews with informants, which included the chairman, secretary, treasurer of LAZISNU and chairman, secretary, treasurer of Yayasan Kotak Amal. Second, by collecting data in the form of reports on changes in funds for 2020 and 2021, vision and mission, organizational structure, Standard Operating Procedures (SOP), and strategic plan documents. The data processing technique in this study is a reduction technique, which is reconfirming whether the data obtained is very relevant or selecting the required data. The results of this research showed that the performance levels of the two institutions, LAZISNU Sidoarjo and Yayasan Kotak Amal Sidoarjo were as follows: LAZISNU's performance level was the highest score achieved in financial performance then management performance. Meanwhile, the lowest performance was found in the performance of social legitimacy programs.Overall, the score obtained is 6.8 or A+, while the level of performance of the  Yayasan Kotak Amal is known that the highest score is achieved in management performance then financial performance. Meanwhile, the lowest performance is in the performance of social legitimacy. Overall, the score obtained is 6.5 or A-.Based on the research above, the researcher gives advice to LAZISNU and Yayasan Kotak Amal to always improve performances which has a major influence on the Amil Zakat Institutions.
The Elevation of Red Blood Cell Distribution Width is an Independent Prognostic Factor for Juvenile Myelomonocytic Leukemia
Authors: Liang Weiru , Liu Chenmeng , Zhang Jingliao , Yi Meihui , Cai Yuli , Zhang Aoli , Liu Lipeng , Zhang Li , Chen Xiaojuan , Zou Yao , Chen Yumei , Guo Ye , Zhang Yingchi , Zhu Xiaofan , Yang Wenyu
Published: December 2022

Juvenile myelomonocytic leukemia (JMML) is an overlapping myeloproliferative and myelodysplastic disorder of infants and early childhood. With heterogeneous genetic background across patients, the existing clinical and laboratory prognostic features are inadequate to predict outcome, posing an urgent need to identify new prognostic indicators. Red cell distribution width (RDW) is a critical parameter reflecting the heterogeneity of erythrocyte size. Recent studies highlighted RDW elevation as a valuable indicator for poor outcomes in various diseases. However, the prognostic role of RDW in JMML remains unclear. From the retrospective analysis of our single-center JMML cohort incorporating a total of 77 patients, we discovered RDW elevation as an independent adverse factor for children with JMML. Multivariate cox proportional hazard models showed that patients with red cell distribution width standard deviation (RDW-SD) >51.65 fl at diagnosis were susceptible to much worse overall survival rate (HR = 5.22, CI = 1.50-18.21, P=0.010). Besides, the combination of RDW elevation and protein phosphatase non-receptor type 11 (PTPN11) mutation was likely to predicted a subgroup with the worst outcomesin our cohort.
Electrostimulation of the white matter of the posterior insula and medial operculum: perception of vibrations, heat, and pain
Authors: Mandonnet Valéry , Rheault François , Descoteaux Maxime , Devaux Bertrand , Levé Charlotte , Froelich Sébastien , Mandonnet Emmanuel
Published: December 2022

This study aimed to characterize the sensory responses observed when stimulating electrically the white matter surrounding the posterior insular point. We thus reviewed patients operated on under awake conditions for a glioma located in the temporo-parietal junction. Patients’ perceptions under electrical stimulation were retrieved from operating reports. Stimulation points were located onto the postoperative MRI and further registered to the MNI template. Twelve stimulation points in eight patients were analyzed. Painful sensations in the leg were reported in 6 cases, close to the posterior insular point. Pain had diverse qualities: burning, tingling, crushing, or electric shock. More laterally, in the white matter of the parietal operculum, heat sensations were described, with a mesio-lateral somatotopy. Finally, vibration sensations were also reported, intermingled with pain and heat areas. In relation to Glasser atlas, pain sites abutted OP2/3 parcel, while heat sites were located within OP1. The white matter surrounding the posterior insula and medial operculum thus constitutes a sensory hub, at the cross-road of triangular connections, linking two at a time the primary somatosensory area, the secondary somatosensory area, and the thalamus. Its surgical preservation is of utmost importance to prevent the postoperative onset of a debilitating insulo-opercular pain syndrome.
Contraceptive use and fertility preferences among sexually active males in Zambia.
Authors: Bwalya Bwalya Bupe , Kasonde Mwewa E. , Mulenga James Nilesh , Mapoma Chabila Christopher , Wamunyima Nayunda , Siamianze Billy , Onukogu Obinna Banda
Published: December 2022

Background: Contraceptive use has been used as one of the methods not only to space but also to limit the number of children that a couple/individuals have. Since their introduction, most of the efforts have concentrated on females than males even though males tend to have huge say on the ideal number of children a couple/individuals have. In our study, we sought to determine linkages between contraceptive use and fertility preferences as well as associated demographic and socio-economic characteristics among sexually active males in Zambia regardless of their marital status.
Methods: The study had two outcome variables, namely contraceptive use and fertility preference (measured by desire for more children as a count variable). Data for this paper was the male recode dataset from the 2018 Zambia Demographic and Health Survey. Binary logistic regression was performed to determine odds ratios of contraceptive use by selected characteristics of males. Poisson regression model was used to establish factors associated with fertility preferences.
Results: Age of men (20-29, 30-39 and 40-49, respectively), residence in rural areas, wealth quintile, religious affiliation of Protestant or Muslim, media exposure, and having discussed Family Planning with a health worker in the last few months were associated with contraceptive use. Males who reported using any contraception method reported 3% less ideal number of children desired compared with those who were not using any method. Older males (age group 30-49), resident in rural areas, with primary education, married, employed, Protestant by religion, and opinionated to the extent of labeling women who use contraceptives “as promiscuous”, had more ideal number of children comparatively.
Conclusions: There were minimal differences in the ideal number of children among males who reported using and not using any contraceptive method. Strategies aimed at encouraging contraception use should cover all categories of males so as to achieve universal involvement of men in family planning in Zambia. A study combining both qualitative and quantitative methods would be ideal to look holistically at the demographic, socio-economic and cultural factors associated with non-contraception use and ideal number of children among males.
Renal Biopsy Diagnosis of Acute Tubular Injury After Pfizer-BioNTech COVID-19 Vaccination: A Case Report
Authors: Soma Yu , Hoshino Kaoru , Sunohara Sumire , Iwano Takehisa , Kawano Naomi
Published: December 2022

Recently, there have been reports of new cases of acute kidney injury (AKI) after coronavirus disease 2019 (COVID-19) vaccination. Podocytic damage, IgA nephropathy, vasculitis, tubulointerstitial damage, and thrombotic microangiopathy have been reported as the causes. However, there are no reports of acute tubular injury (ATI) as the sole cause of AKI. In this case, a 54-year-old man with type 2 diabetes visited a local physician. He was highly obese with a body mass index of 36 kg/m2. He was treated with metformin and insulin. Diabetic retinopathy, urinary protein, and occult blood were absent. He had received four COVID-19 vaccines; three were from Pfizer and one from Moderna. He was referred to our hospital 5 days after receiving the fourth dose of the Pfizer-BioNTech COVID-19 vaccine. He had stage 3 AKI. Urinary findings revealed the presence of new proteinuria and glomerular occult blood. Steroids were introduced on the day of admission for rapidly progressive glomerulonephritis. A renal biopsy was performed on the second day, with results obtained on the fifth day revealing no findings other than ATI. The patient was therefore deemed unamenable to steroids. After steroid discontinuation, renal function recovered spontaneously, and urinalysis abnormalities disappeared. In this case, ATI was the sole pathogenesis of COVID-19 vaccine-induced AKI, and treatment with immunosuppressive drugs was not necessary.
VDR attenuate ischemia-reperfusion kidney injury via inhibiting ERS effector protein ATF4
Authors: Tang Shiqi , Wu Xueqin , Dai Qing , Li Zhi , Yang Shikun , Liu Yan , Liu Jishi , Wang Jianwen , Zhang Wei , Zhang Hao
Published: December 2022

Activating transcription factor 4 (ATF4) is one of the key effectors of endoplasmic reticulum stress (ERS), ATF4/CHOP pathway mediated ERS plays an important role in the progression of acute kidney disease (AKI). We have previously reported that Vitamin D receptor (VDR) exert renoprotection in rodent AKI models. However, whether ATF4 as well as ERS is involved in the protective effect of VDR in ischemia-reperfusion (I/R) induced AKI unknown. Herein, we showed that VDR agonist paricalcitol and VDR overexpression alleviated I/R induced renal injury and cells apoptosis with decreased ATF4 and attenuated ERS, while VDR deletion significantly resulted in further increased ATF4, more drastic ERS and renal injury in I/R mice models. In addition, paricalcitol remarkably reduced tunicamycin™ induced ATF4 and ERS with attenuated renal injury, while VDR deletion aggravated the above changes in TM mice models. Moreover, overexpression of ATF4 partially abolished the effect of paricalcitol against TM-induced ERS and apoptosis, while inhibition of ATF4 enhanced the protective effect of paricalcitol. Bioinformatics analysis indicated potential VDR binding sites on ATF4 promotor sequence which were further confirmed by ChIP-qPCR and dual-luciferase reporter gene assay. In conclusion, VDR attenuated I/R-induced AKI through suppressing ERS partly via transcriptional regulation of ATF4.
β-Sitosterol alleviates neuropathic pain by affect microglia polarization through inhibiting TLR4/NF-κB signaling pathway
Authors: Zheng Yachun , Chang Shiquan , Zhang Di , Shi Huimei , Li Xin , Chen Zenni , Jing Bei , Zhao Guo-Ping
Published: December 2022

The etiology of neuropathic pain is mostly due to mechanical deformation and neuroinflammation, of which neuroinflammation is the main cause of chronic neuropathic pain. Activation of the TLR4/NF-κB signaling pathway mediates elevated levels of inflammatory cytokines, and we clearly demonstrated by in vivo and in vitro Western blot experiments that β-sitosterol significantly inhibited the elevated TLR4 expression levels and NF-κB activation associated with inflammatory responses. In cellular experiments, we could clearly see that both β-sitosterol and TLR4/NF-κB signaling pathway inhibitor could inhibit M1 pro-inflammatory phenotype expression and promote M2 anti-inflammatory phenotype expression in microglia GMI-R1 by flow cytometry and immunofluorescence assays. Therefore, we suggest that β-sitosterol can affect microglia polarization by inhibiting TLR4/NF-κB signaling pathway thereby reducing neuroinflammation and thus alleviating neuropathic pain.
Reliability of Prostate Imaging Reporting and Data System Version 2.1 for Excluding Clinically Significant Prostate Cancer Using a 1.5 Tesla Scanner
Authors: Sharqawi Abdallah , Drye Naomi , Shugaba Abdul , O’reilly Alison , El-Sakka A I
Published: December 2022

Introduction
Multiparametric magnetic resonance imaging (mpMRI) of the prostate gland is now the recommended initial investigation of choice for the detection of Prostate cancer (PCa). It effectively identifies patients who require prostate biopsies due to the risk of clinically significant PCa. It helps patients with clinically insignificant PCa avoid the invasive biopsies and possible accompanying complications.
Large clinical trials have investigated the accuracy of mpMRI in detecting PCa. We performed a local review to examine the reliability of omitting tissue sampling in men with a negative (PIRADS 2 (P2) or less) mpMRI in the primary diagnostic setting.
Methods
This was a retrospective study of patients with clinical suspicion of PCa within a 2-year period. Patients had a mpMRI prior to having trans-perineal prostate gland biopsies. Clinically significant disease was defined as Gleason 7 and above. Data was analysed using chi square and student t test and a p value of <0.05 was deemed significant.
Results
Out of 700 patients 90 had an mpMRI score of PIRADS 2. Seventy-seven (85%) of these patients had a negative biopsy, 9 (10%) showed Gleason 6 and 4 patients showed Gleason 7 or above. 79 patients with PIRADS 2 had a PSA density of <0.15, none of which had a clinically significant biopsy result. The negative predictive value of mpMRI from this study is 95%.
Conclusion
Our results are in line with negative predictive values demonstrated in the current literature. This local study, likely applicable to other district general hospitals, shows that mpMRI is a safe and reliable initial investigation to aid decisions on which patients require biopsies.
Surface modification of magnetite using silica coating: Spectroscopic, structural, morphological characterization and interaction with Cristal Violet dye
Authors: Andolsi Amal , Islem Chaari , Hamzaoui Ahmad Hichem
Published: December 2022

The essential goal of this work is to produce an eco-friendly and economically nano-adsorbent that may separate organic dye, especially, cationic dye, from polluted water prior to making use of this adsorbent in industrial filed. This work suggests a way for fabricating magnetite and silica. The proposed approach concerned three steps : the preparation of magnetite (Fe3O4) nanoparticles by co-precipitation method, then of silica using sodium silicate prepared from siliceous sand, and finally magnetite coating of silica Fe3O4/SiO2. The nanocomposite Fe3O4@SiO2 were investigated by XRD, BET, FT-IR and TEM. The XRD characterization displayed that silica formed an amorphous phase and that magnetite shaped a spinel phase. To optimize the diverse experimental variables affecting the elimination performance of CV, the effects of four experimental parameters including solution pH, adsorbent amount, contact time and ionic strength were evaluated. Adsorption kinetic disclosed that pseudo-second-order is the best model (R2 > 0.99, qe,cal = 6.68 mg g− 1). The negative ΔH° and ΔG° values exhibited the exothermic and spontaneous nature of CV sorption on the nanoparticles, respectively.
Imaging of Atherosclerosis with [64Cu]Cu-DOTA-TATE: a Translational Head-to-Head Comparison Study with 2-[18F]FDG, and Na[18F]F in Rabbits
Authors: Grandjean Constance E. , Pedersen Sune F. , Christensen Camilla , Dibenedetto Altea , Eriksen Thomas , Binderup Tina , Kjaer Andreas
Published: December 2022

Atherosclerosis is a chronic inflammatory disease of the larger arteries that may lead to cardiovascular events. Identification of patients at highest risk of cardiovascular events is challenging, but molecular imaging using positron emission tomography (PET) may prove useful.
The aim of this study was to evaluate and compare head-to-head three different PET tracers. Furthermore, tracer uptake is compared to gene expression alterations of the arterial vessel wall.
Methods
Male New Zealand White rabbits (control group; n=10, atherosclerotic group; n=11) were used for the study. Vessel wall uptake was assessed with the three different PET tracers: 2-[18F]FDG (inflammation), Na[18F]F (microcalcification), and [64Cu]Cu-DOTA-TATE (macrophages), using PET/Computed Tomography (CT). Tracer uptake was measured as standardized uptake value (SUV), and arteries from both groups were analyzed ex vivo by autoradiography, qPCR, histology, and immunohistochemistry.
Results
In rabbits, the atherosclerotic group showed significantly higher uptake of all three tracers compared to the control group 2-[18F]FDG: SUVmean 1.50 ± 0.11 vs. 1.23 ± 0.09, p = 0.025; Na[18F]F: SUVmean 1.54 ± 0.06 vs. 1.18 ± 0.10, p = 0.006; and [64Cu]Cu-DOTA-TATE: SUVmean 2.30 ± 0.27 vs. 1.65 ± 0.16; p = 0.047. Of the 102 genes analyzed, 52 were differentially expressed in the atherosclerotic group compared to the control group and several genes correlated with tracer uptake.
Conclusion
In conclusion, we demonstrated the diagnostic value of [64Cu]Cu-DOTA-TATE and Na[18F]F for identifying atherosclerosis in rabbits. The two PET tracers provided information that could not be obtained with 2-[18F]FDG.
Research on wheel-legged robot based on LQR and ADRC
Authors: Liu Shuaishuai , Yuan Qiang , Wang Bangyu , Xiao Junbo , Zhao Daxu
Published: December 2022

The traditional two-wheeled self-balancing robot can travel quickly in a flat road environment, and it is easy to destabilize andcapsize when passing through a bumpy road. To improve the passing ability of a two-wheeled robot, a new wheel-leggedtwo-wheeled robot is developed. A seven-link leg structure is proposed through the comprehensive design of mechanismconfiguration, which decouples the balanced motion and leg motion of the robot. Based on the Euler-Lagrange method, thedynamic model of the system is obtained by applying the nonholonomic dynamic Routh equation in the generalized coordinatesystem. The robot’s state space is divided according to the robot’s height, and the Riccati equation is solved in real-time by thelinear quadratic regulator(LQR) method to complete the balance and motion control of the robot. The robot leg motion controlis achieved based on the Active Disturbance Rejection Control(ADRC) way. A robot simulation model is built on Recurdyn toverify the algorithm’s feasibility, and then an experimental prototype is built to demonstrate the algorithm’s effectiveness. Theexperimental results show that the control method based on LQR and ADRC can make the robot pass through the bumpy road.
Stoma Acceptance and Its Related Factors Among Colorectal Cancer Patients：A Descriptive Study
Authors: Jing Xuebing , Han Qingkun , Han Wei , Gao Tingting , Li Cuiping , Wei Jingwen , Yang Xiaoping
Published: December 2022

Purpose: The aim of the study is to determine the levels and related factors of colorectal cancer patients about stoma acceptance.
Methods: This descriptive study enrolled 136 colorectal cancer outpatients follow-up at several hospitals in Shandong province. Stoma acceptance was assessed with Chinese version stoma acceptance questionnaire(SAQ). The association of stoma acceptance and colorectal cancer patients characteristics was assessed by logistic regression.
Results: The total average stoma acceptance of 136 patients in this study was(37.03±4.87). General self-efficacy was(27.96±5.28). General self-efficacy was positively associated with stoma acceptance(r=0.529，P<0.001). The linear regression results showed that factors of general self-efficacy, social needs, receiving medical follow-up and education level were the factors affecting stoma acceptance in colorectal cancer patients.
Conclusions: The stoma acceptance in colorectal cancer patients in grade 3 hospitals is at a medium level in China. Medical staff should pay attention to it, and improve caregiver’ supports, increase medical follow-up,meet the need for social communication. Strengthen the intervention of patients’ self-efficacy to intervene, so as to improve the stoma acceptance and improve the quality of life.
The Long-term impact of Covid-19 on student mental health
Authors: Catling Jon C.
Published: December 2022

Background
The COVID-19 pandemic and the resulting restrictions placed upon society have had a profound impact on both physical and mental health, particularly for young people.
Aims
The current study assesses student mental health pre, within and post COVID.
Method
Eight hundred and fourteen first year Undergraduate students completed a battery of self-report questionnaires to assess for depression, mobile phone use, self-esteem, resilience and social support with data being collected over a three year period. The data from each year was compared (216, 218 and 380 students respectively).
Results
A MANOVA revealed that COVID-19 had a significant impact on self-reported levels of depression, smartphone use, Self-esteem, resilience and social support. Multiple regression analysis revealed that Smart phone use and resilience were significant predictors of levels of depression.
Conclusions
This research suggests that COVID-19 has had a major impact upon student mental health, and that we have not yet seen a recovery in our ‘post-Covid’ times.
Comparison of Insulin Resistance and Overian Volume in Patients with Polycystic Ovary Syndrome with and without Hashimoto
Authors: Gencer Gülcan , Serin Aliye Nigar , Gencer Kerem
Published: December 2022

Objective: In this study, our aim was to divide the patients diagnosed with polycystic ovary syndrome (PCOS) and Hashimoto's thyroiditis (HT) into subgroups according to different clinical and laboratory findings. It is to investigate whether it will exacerbate it.
Material and Methods: 46 PCOS patients and 46 PCOS patients diagnosed with HT were included in this study. A detailed medical history was taken from all participants. Polycystic ovary image was evaluated as below or above 10 ml and antral follicles were counted by transvaginal ultrasound. Insulin resistance of the patients was evaluated according to the fasting insulin (HOMA) index.
Results: Insulin resistance was found to be associated with fasting insulin, HOMA index, body mass index and right ovarian volume in patients diagnosed with PCOS. Among the patients diagnosed with PCOS+HT, insulin resistance was found to be significantly correlated with fasting insulin, HOMA index, (BMI), (SHBG) and left ovarian volume. An increase in right ovarian volume was found in 37.5% of patients with PCOS without insulin resistance and in 76.3% of patients with insulin resistance. An increase in left ovarian volume was found in 35.7% of patients without insulin resistance diagnosed with PCOS+HT and in 68.8% of patients with insulin resistance.
Conclusions: This study shows that in order to predict insulin resistance that causes long-term metabolic diseases, ovarian volume should be evaluated in every PCOS patient and all PCOS patients with increased ovarian volume should be investigated for insulin resistance. In addition, it has been observed that insulin resistance affects left ovarian volume in patients with PCOS+HT, while insulin resistance affects the volume of the right ovary more in patients with PCOS. At least one ovary has been found to be affected by long-term metabolic diseases.
Prevalence and Risk Factors of Bovine Tuberculosis in Cattle in Selected Districts of Fafan Zone, Eastern Ethiopia
Authors: Hussein Hassan Abdi , Musse Abdi Hussein
Published: December 2022

Cross-sectional study was conducted from October 2021 to August 2022, to investigate the prevalence and associated risk factors of bovine tuberculosis in cattle in selected districts of Fafan zone. The prevalence was 11.24% (95% CI, 8.61–14.35) and 43.3% (95% CI, 33.27–53.75) at individual and herd level respectively. There were statistically significant differences in proportions of positive reactor animals among body condition score (χ2 = 47.9, P = 0.000), Age (χ2 = 6.0937, P = 0.048), Seasonal migration (χ2 = 4.320, P = 0.038), parity number (χ2 = 10.62, P = 0.005), reproductive status (χ2 = 4.36, P = 0.037). Multivariable logistic regression analysis showed that animal with poor body condition score was more likely to react positive than those in good body condition score adjusted (OR = 0.163; CI = 0.0679 -0.390). Moreover, seasonally migrating cattle had 2.787 times the odds of being tuberculin reactors compared with those non migrating cattle (adjusted OR = 2.787; CI = 1.221–6.358). According to questionnaire, 14.2% (17 of 120) and 13.3% (16 of 120) of the respondents were knowledgeable about bovine tuberculosis and its transmission from animal to human and vice versa, respectively. The general judgment of herders' understanding of BTB transmission methods to humans was very low. This finding provides relatively high prevalence of BTB in cattle at the study area and highlights the need for a practicable control strategy of the disease in the region.
Sex difference in the cytokine profile among patients hospitalized for COVID-19 and during their recovery: predominance of females in adhesion molecules and males in oxidative stress
Authors: Mitrović-Ajtić Olivera , Djikić Dragoslava , Subotički Tijana , Bižić-Radulović Sandra , Beleslin-Čokić Bojana , Dragojević Teodora , Živković Emilija , Miljatović Sanja , Čokić Vladan
Published: December 2022

Background
Male sex is associated with greater severity and mortality from coronavirus disease 2019 (COVID-19), although infections are equally distributed between men and women. The present study aims to investigate sex differences in the hyperinflammatory immune response to SARS-CoV-2 infection and consequent thrombosis using linked cytokine profile and blood laboratory data.
Methods
This observational cohort study involved 99 COVID-19 patients (69 male and 30 female adults), hospitalized between March 2021 and April 2022. Their clinical and laboratory data were collected to examine sex difference in oxidative stress, neutrophil extracellular traps (NETs) formation and plasma cytokines at hospital admission and up to 5 months of their recovery.
Results
Dihydrotestosterone (DHT) levels were transiently reduced, while sex hormone binding globulin levels were continuously decreased in male post-COVID-19 patients after its rise at diagnosis. Regarding inflammatory cytokines, interleukin-6 (IL-6) and interferon-gamma are generally increased at diagnosis, while IL-6 is decreasing in post-COVID-19 patients. Tumor necrosis factor-alpha had 5-fold increase in females at diagnosis. Chemokines IL-8 and monocyte chemoattractant protein-1 and coagulation markers intercellular adhesion molecule-1 and E-selectin were consistently upregulated in female COVID-19 and post-COVID-19 patients, in contrast to vascular cell adhesion molecule-1 and P-selectin. DHT increased reactive oxygen species in neutrophils of male patients, while estrogen decreased in females. Markers for NETs formation, such as circulating DNA and myeloperoxidase, were significantly increased in the plasma of patients. Sex hormones are mostly negatively correlated with leukocytes, while in positive correlation with coagulation markers.
Conclusions
Markers of chemotaxis, endothelial dysfunction and inflammation are still detectable and partially sex dependent in COVID-19 patients after 5 months of hospital admission. These distributions of sex steroid hormones splitted endothelial adhesion molecules, coagulation and inflammation factors, while they generalized chemokines.
Wnt Signaling Pathway Related Gene CTNNBIP1 Act as A potential Prognostic Biomarker and A Comprehensive Prognostic Analysis in Osteosarcoma
Authors: Wen Jian , WAN Lijia , Dong Xieping
Published: December 2022

Purpose
Osteosarcoma is the most common primary bone malignancy in children and young adults with poor outcomes in patients with recurrence and metastasis. The role of Wnt signaling pathway in osteosarcoma was complex and not well specified.
Methods
KEGG Wnt signaling pathway related genes were screened by univariate Cox regression analysis, Boruta feature selection and KM analysis in TARGET-OS cohort for the gene with the highest prognostic value in osteosarcoma. Clinical and immune cell infiltration features were also screened by univariate Cox regression, respectively. A prognostic model was established by the indicators screened above and evaluated in training and validation sets. Then, functional analysis of the differentially expressed genes were performed.
Results
CTNNBIP1 was screened as the KEGG Wnt signaling pathway related gene with the highest prognostic value in osteosarcoma. Metastasis, tumor site and M2 macrophage infiltration in tumor tissue were also identified as prognostic indicators. The model established by these indicators was evaluated in training set and successfully validated in validation set. C-index (0.812 in training set and 0.787 in validation set) and Calibration analysis suggested that the model had high prediction accuracy both in training and validation set. AUCs of the ROC analysis for 1-,3-,5-year prediction of the model were 0.96, 0.81 and 0.81 in training set, 0.80, 0.83 and 0.89 in validation set. Decision curve analyses also showed that patients could get high net benefit from the model. Functional analyses of the differential expressed genes between normal and osteosarcoma patients indicated that Wnt signaling pathway and cell cycle were upregulated and focal adhesion was downregulated in osteosarcoma patients. The overexpression of CTNNBIP1 in osteosarcoma tissues was validated by immunohistochemistry.
Conclusions
CTNNBIP1 was a potential prognostic gene and therapeutic target in osteosarcoma. Prognostic model established by CTNNBIP1, clinical and immune cell infiltration indicators had good prediction accuracy and discriminatory capacity.
A Neonate with Multiple Hand Flexor Tendon Ruptures due to Methicillin-susceptible Staphylococcus aureusSepsis: A Case Report
Authors: Nakamura Tomomi , Iwai Masanori , Inoue Takeshi , Irie Hiroki , Karasugi Tatsuki , Seki Atsuhito , Hamaguchi Masayoshi , Kuraoka Shohei , Mizukami Tomoyuki , Nakamura Kimitoshi
Published: December 2022

Background: Neonatal pyogenic tenosynovitis is a highly emergent soft tissue infection. We report a case of a neonate with pyogenic tendinopathy and tendon rupture diagnosed by ultrasonography (US). He subsequently developed pyogenic arthritis and osteomyelitis during antimicrobial therapy.Case presentation: A 7-day-old boy was admitted to our hospital with redness and swelling of the right index finger. US on admission showed rupture of the flexor tendon of the right index finger with inactivity. The day after admission, he developed pyogenic arthritis of the right elbow and, subsequently, pyogenic osteomyelitis.Staphylococcus aureuswas identified through bacterial culture, and the patient was treated with intravenous antibiotics for 6 weeks. However, after discharge from our hospital, rupture of the flexor tendon of the left thumb was confirmed. A two-stage flexor tendinoplasty was completed at the age of 2 years and 1 month for the flexor tendon rupture on his right index finger.Conclusions: In addition to blood culture, ultrasonographic evaluation should be performed in neonates with erythematous and swollen joints to identify the focus of infection as soon as possible. Moreover, repeated regular US examination is important in the follow-up of bone and soft tissue infections.
Multifocal brain abscess caused by Aspergillus fumigatus infection in patients with Heat Stroke: a case report
Authors: Ding Peng , Zhou Yuan , Wang Chunxia , Zhou Xiujuan , Long Kunlan , Gao Peiyang
Published: December 2022

Background:
Aspergillus fumigatus is a filamentous fungus widely distributed in the environment and can cause life-threatening diseases in individuals with compromised immune barriers. To our knowledge, it has never been reported as a cause of intracranial infection in patients with heat stroke. We report a case of heat stroke patient with multifocal brain abscess caused by aspergillus fumigatus infection.
Case presentation:
A 48-year-old male patient was admitted to the intensive care unit due to fever and loss of consciousness. The patient was diagnosed with heat stroke because he worked in a high-temperature environment, had a hospital temperature of 42℃, had liver, kidney and heart damage, and had disseminated intravascular coagulation. Although the patient was actively rescued, the patient was still coma and fever. Head Computed tomography (CT) and magnetic resonance imaging ( MRI ) showed abnormal signal lesions in bilateral basal ganglia, right temporal lobe, left frontal lobe and left cerebellar hemisphere. Serum galactomannan increased. It was identified as Aspergillus fumigatus by next generation sequencing ( NGS ) using cerebrospinal fluid. The patient received 4 weeks of voriconazole treatment. Reexamination of head CT suggested that the range of low-density lesions in the above brain regions was reduced. Then the patient gradually woke up, could follow the instructions of exercise, and finally discharged.
Conclusion:
Heat stroke combined with multifocal brain abscess caused by aspergillus fumigatus infection is extremely rare. For patients who still have central nervous system symptoms and signs after conventional treatment, the possibility of intracranial infection needs to be considered.
A qualitative exploration of food portion size practices and awareness of food portion size guidance in first-time parents of one- to two-year-olds living in the UK
Authors: Porter Alice , Langford Rebecca , Summerbell Carolyn , Tinner Laura , Kipping Ruth
Published: December 2022

Background
Food portion size guidance resources aimed at parents of young children in the UK are freely available from a number of credible sources. However, little is known about whether parents are aware of, and use, any of these resources to guide their food portioning practices.
Objectives
We aimed to explore the food portion size practices used by first-time parents living in the UK when feeding their one- to two-year-old child, and also their awareness of and views on six food portion size guidance resources.
Methods
Participants were recruited via parent Facebook groups and online parent forums. Online 1-1 semi-structured interviews were conducted, during which parents were shown images of six food portion sizeguidance resources to facilitate discussion. Data was analysed in NVivo 11 using a Reflexive Thematic Analysis approach.
Results
Of the 27 participants, most were women (n=25), white (n=18), and educated to a first degree level or higher (n=24). First-time parents mostly relied on their own judgement and “instinct” to portion foods, based on their learned experience of how much their child ate on a day-to-day basis. This experience was used alongside physical indicators of food portion size, such as the size of children’s dishware and food packaging. Most participants were unaware of any of the six food portion size guidance resources we showed them; only four had read any of the resources. Parents suggested they had previously sought advice about weaning from a range of sources (e.g. online, friends, community groups) but would be unlikely to seek out specific food portion size guidance. Parents suggested recommendations on food portion size should acknowledge and highlight parents’ perception that “every child is different”.
Conclusions
Existing food portion size guidance resources for parents of young children in the UK are ineffective as they have poor reach and impact. We suggest parents should be involved in developing novel strategies to promote age-appropriate consumption and healthy weight gain in young children.
Malaria Vaccine Impact on Cases, Drug-resistant Cases, and Deaths in Africa: A Modeling Study
Authors: Hamilton Alisa , Haghpanah Fardad , Hasso-Agopsowicz Mateusz , Frost Isabel , Lin Gary , Schueller Emily , Klein Eili , Laxminarayan Ramanan
Published: December 2022

Background
The emergence of antimalarial drug resistance poses a major threat to effective malaria treatment and control in sub-Saharan Africa. The RTS, S/AS01 vaccine has the potential to reduce both resistant infections and antimalarial use. Modeling studies projecting aggregate health burden averted under different scenarios can support further vaccine development and implementation.
Methods
A mathematical model projecting cases, drug-resistant cases, and deaths averted from 2021 to 2030 with a vaccine against clinical malaria caused by Plasmodium falciparum administered yearly to one-year-olds in the WHO Africa Region.
Findings
Under a scenario in which vaccine efficacy (VE) was constant at 40% for four years and dropped to 0% in year five, approximately 92.5 million cases, 700,000 resistant cases, and 253,000 deaths were averted by 2030. In a scenario in which VE began at 80% and dropped 20 percentage points each year, approximately 123 million cases, one million resistant cases, and 336,000 deaths were averted. The highest burden averted occurred when VE remained 40% for 10 years with approximately 151 million cases, 1.1 million resistant cases, and 411,000 deaths averted. In a scenario of rapidly increasing drug resistance and an effective vaccine, over 4.5 million resistant cases were averted.
Interpretation
Swift and widespread deployment of an effective malaria vaccine in Africa, alongside other prevention and control interventions, could substantially reduce health and economic burden caused by drug-resistant malaria.
Funding
This work was funded by a grant from the Bill & Melinda Gates Foundation (OPP1190803) to the Center for Disease Dynamics, Economics & Policy under the ARVac Consortium.
De novo design of a novel AIE fluorescent probe tailored to autophagy visualization via pH manipulation
Authors: Huang Xueyan , Chen Fei , Ma Yeshuo , Zheng Fan , Fang Yanpeng , Feng Bin , Huang Shuai , Zeng Hongliang , Zeng Wenbin
Published: December 2022

Background
Macroautophagy is an essential cellular self-protection mechanism, and defective autophagy has been considered to contribute to a variety of diseases. During the process, cytoplasmic components are transported via autophagosomes to acidic lysosomes for metabolism and recycling, which represents application niches for lysosome-targeted fluorescent probes. And in view of the complexity of the autophagy pathway, it entails more stringent requirements for probes suitable for monitoring autophagy. Meanwhile, aggregation-induced emission (AIE) fluorescent probes have been impressively demonstrated in the biomedical field, which bring fascinating possibilities to the autophagy visualization.
Methods
We reported a generalizable de novo design of a novel pH-sensitive AIE probe ASMP-AP tailored to lysosome targeting for the interpretation of autophagy. Firstly, the theoretical calculation was carried out followed by the investigation of optical properties. Then, the performance of ASMP-AP in visualizing autophagy was corroborated by starvation or drugs treatments. Furthermore, the capability of ASMP-AP to monitor autophagy was demonstrated in ex vivo liver tissue and zebrafish in vivo.
Results
ASMP-AP displays a large stokes shift, great cell permeability and good biocompatibility. More importantly, ASMP-AP enables a good linear response to pH, which derives from the fact that its aggregation state can be manipulated by the acidity. It was successfully applied for imaging autophagy in living cells and was proved capable of monitoring mitophagy. Moreover, this novel molecular tool was validated by ex vivo visualization of activated autophagy in drug-induced liver injury model. Interestingly, it provided a meaningful pharmacological insight that the melanin inhibitor 1-phenyl-2-thiourea (PTU)-induced autophagy was clearly presented in wild-type zebrafish.
Conclusions
ASMP-AP offers a simple yet effective tool for studying lysosome and autophagy. This is the first instance to visualize autophagy in zebrafish using a small-molecule probe with AIE characters, accurate lysosome targeting and simultaneous pH sensitivity. Ultimately, this novel fluorescent system has great potential for in vivo translation to fuel autophagy research.
Feasibility of hip dysplasia screening in primary care clinics via AI-augmented point-of-care ultrasound.
Authors: Jaremko Jacob , Hareendranathan Abhilash Rakkunedeth , Bolouri Ehsan Seyed , Frey Rod , Dulai Sukhdeep , Bailey Allan
Published: December 2022

Background: Developmental dysplasia of the hip (DDH) is a common cause of premature osteoarthritis. This osteoarthritis can be prevented if DDH is detected by ultrasound and treated in infancy, but universal DDH screening is generally not cost-effective due to the need for experts to perform the scans.
Questions/purposes: The purpose of our study was to evaluate the feasibility of having non-expert primary care clinic staff perform DDH ultrasound using handheld ultrasound with artificial intelligence (AI) decision support.
Patients and Methods: We performed an implementation study evaluating the FDA-cleared MEDO-Hip AI app interpreting cine-sweep images obtained from handheld Philips Lumify probe to detect DDH. Initial scans were done by nurses or family physicians in 3 primary care clinics, trained by video, powerpoint slides and brief in-person. When the AI app recommended follow-up, we first performed internal follow-up by a sonographer using the AI app; cases still considered abnormal by AI were referred to pediatric orthopedic clinic for assessment.
Results: We performed 369 scans in 306 infants. Internal follow-up rates were initially 40% for nurses and 20% for physicians, declining steeply to 14% after ~60 cases/site: 4% technical failure, 8% normal at sonographer follow-up using AI, and 2% confirmed DDH. Of 6 infants referred to pediatric orthopedic clinic, all were treated for DDH (100% specificity); 4 had no risk factors and may not have otherwise been identified.
Conclusions: Real-time AI decision support and a simplified portable ultrasound protocol enabled lightly trained primary care clinic staff to perform hip dysplasia screening with follow-up and case detection rates similar to costly conventional ultrasound screening performed by experts. This highlights the potential utility of AI-supported portable ultrasound in primary care.
Level of Evidence: This study uses data from consecutive patients and would be classified as Level 3 evidence
Interrogating microglial engulfment of synaptic terminals and myelin using FEAST
Authors: Dissing-Olesen Lasse , Walker Alec , FENG Qian , Barr Helena , Walker Alicia , Xie Lili , Wilton Daniel , Das Indrani , Benowitz Larry , Stevens Beth
Published: December 2022

Although engulfment is a hallmark of microglia function, fully validated platforms that facilitate high-throughput quantification of this process are lacking. We therefore developed FEAST (Flow cytometric Engulfment Assay for Synaptic Terminals) to enable interrogation of in vivo engulfment of synaptic material by brain resident macrophages at single-cell resolution. We optimized FEAST for two different analyses: quantification of fluorescent material inside live cells and of engulfed endogenous proteins within fixed cells. To overcome false-positive engulfment signal, we introduced an approach suitable for interrogating engulfment in microglia from perfusion-fixed tissue. As a proof-of-concept for the specificity and versatility of FEAST, we examined the engulfment of synaptic proteins after optic nerve crush as well as myelin in two models of demyelination and found that microglia, but not other brain-associated macrophages, engulf in these contexts. Our work underscores how FEAST can be utilized to gain critical insight into functional neuro-immune interactions that shape development, homeostasis, and disease.