bluuebunny/crossref_metadata_2025 huggingface dataset:
Initial Evaluation of the Merit of Guar as a Dairy Forage Replacement Crop during Drought-Induced Water Restrictions
Authors: Ramos Coronado Luis , Miller Maryfrances , Angadi Sangu V. , Lauriault Leonard M.
Published: May 2024

Dairy is the largest sector of the agricultural industry in New Mexico, but the sector’s need for high quality forage in the face of ongoing drought and climate change causes concern. The economic merit of using guar to replace alfalfa under imposed constraints in irrigation water availability is evaluated. Nutritional quality of guar (Cyamopsis tetragonoloba L.) grown at the NMSU Agricultural Science Center at Clovis was estimated using Near Infrared Spectroscopy (NIRS) analysis. Results show that 45.6 percent of analyzed samples were comparable to Supreme or Premium alfalfa (Medicago sativa) hay using Relative Feed Value index, and 23.7% met this criteria using crude protein. While alfalfa uses 4.4 acre-feet of water (approximately 5400 cubic meters) per season, the guar sampled used 5 inches (500 cubic meters) of applied irrigation. Microsoft Excel Version 2404 LP Solver was used to identify an optimum crop mix of alfalfa and guar under different levels of irrigation water constraints. With increasing levels in modeled irrigation restrictions, the use of guar increased the potential forage production by 3% under a 0.4 acre-foot restriction up to almost 59% under a 1.5 acre-feet irrigation water limit. Our results merit additional research, including forage trials to determine the impact of guar forage on milk production and further agronomic research into growing guar to maximize forage yield.
Impacts of Farming Activities on Nitrogen Degradability under a Temperate Continental Monsoon Climate
Authors: Wang Qiuju , Zou Jiahe , Liu Yanxia , Li Jingyang , Liu Xin , Zhang Haibin , Jiao Feng , Meng Qingying , Guo Zhenhua
Published: May 2024

Nutrient fertilizer application to agricultural land has led to greenhouse gas emissions and has altered soil nitrogen (N) deposition. In soil, N can be degraded in four ways: entering surface water through water flow, absorption by plants and microorganisms, decomposition into gas, and deposition as minerals. This study proposes the concept of N degradability and aims to clarify how farming activities affect N degradability in soil. Over 260 soil profiles were excavated, and the effective soil depth, coordinates, soil types, and vegetation were recorded at each measurement point. The following characteristics were determined in the soil samples: pH, organic matter, total N, total phosphorus, total potassium, total soluble N, available phosphorus, and available potassium. The sample characteristics were subjected to Pearson correlation analysis, principal component analysis, and one-way analysis of variance. The 260 samples included four soil types: dark brown soil, black soil, albic black soil, and meadow soil. Black soil exhibited more stable N levels compared with the other three soil types, showing a tendency towards N accumulation. Ground vegetation was categorized into seven types: forest, rice, maize, red adzuki bean, grassland, soybean, and others. Forests contributed the most to N deposition. Conversely, planting maize led to a tendency for N loss compared with forests. This study can provide a reference for the sustainable development of agriculture and the balance of ecological protection.
eDNA Metabarcoding Reveals the Species–Area Relationship of Amphibians on the Zhoushan Archipelago
Authors: Li Wenhao , Hou Xianglei , Zhu Yunlong , Du Jiacong , Xu Chunxia , Yang Jingyuan , Li Yiming
Published: May 2024

The species–area relationship is important for understanding species diversity patterns at spatial scales, but few studies have examined the relationship using environmental DNA (eDNA) techniques. We investigated amphibian diversity on 21 islands of the Zhoushan Archipelago and nearby mainland areas in China using the combination of eDNA metabarcoding and the traditional line transect method (TLTM) and identified the species–area relationship for amphibians on the islands. The mean detection probability of eDNA is 0.54, while the mean detection probability of TLTM is 0.24. The eDNA metabarcoding detected eight amphibian species on the islands and nine species in the mainland areas, compared with seven species on the islands and nine species in the mainland areas that were identified by TLTM. Amphibian richness on the islands increased with island area and habitat diversity. The species–area relationship for amphibians in the archipelago was formulated as the power function (S = 0.47A0.21) or exponential function (S = 2.59 + 2.41 (logA)). Our results suggested that eDNA metabarcoding is more sensitive for the detection of amphibian species. The combined use of eDNA metabarcoding and the traditional line transect method may optimize the survey results for amphibians.
A New Cell Line from the Brain of Red Hybrid Tilapia (Oreochromis spp.) for Tilapia Lake Virus Propagation
Authors: Mohamad Aslah , Khemthong Matepiya , Trongwongsa Pirada , Lertwanakarn Tuchakorn , Setthawong Piyathip , Surachetpong Win
Published: May 2024

Tilapia lake virus (TiLV) presents a substantial threat to global tilapia production. Despite the development of numerous cell lines for TiLV isolation and propagation, none have been specifically derived from red hybrid tilapia (Oreochromis spp.). In this study, we successfully established a new cell line, RHTiB, from the red hybrid tilapia brain. RHTiB cells were cultured for 1.5 years through over 50 passages and demonstrated optimal growth at 25 °C in Leibovitz-15 medium supplemented with 10% fetal bovine serum at pH 7.4. Morphologically, RHTiB cells displayed a fibroblast-like appearance, and cytochrome oxidase I gene sequencing confirmed their origin from Oreochromis spp. Mycoplasma contamination testing yielded negative results. The revival rate of the cells post-cryopreservation was observed to be between 75 and 80% after 30 days. Chromosomal analysis at the 25th passage revealed a diploid count of 22 pairs (2n = 44). While no visible cytopathic effects were observed, both immunofluorescence microscopy and RT-qPCR analysis demonstrated successful TiLV propagation in the RHTiB cell line, with a maximum TiLV concentration of 107.82 ± 0.22 viral copies/400 ng cDNA after 9 days of incubation. The establishment of this species-specific cell line represents a valuable advancement in the diagnostic and isolation tools for viral diseases potentially impacting red hybrid tilapia.
Evidence of the Existence of Site-Specific Female Contact Pheromones Involved in the Sexual Interaction Behavior of the Pacific Whiteleg Shrimp Penaeus vannamei
Authors: Gutiérrez-Vera José A. , Ponce-Rivas Elizabeth , Braga André , Paniagua-Chávez Carmen G. , Alfaro-Montoya Jorge , Rosales-Leija Misael
Published: May 2024

Although the presence of female contact sex pheromones in P. vannamei has been hypothesized, to date its existence has not been proven. To gather more evidence of their existence, cuticular liposoluble extracts were obtained from the following samples of adult females to be used as the experimental treatments: (1) ventral exoskeleton of immature female (VI), (2) dorsolateral exoskeleton of immature female (DI), (3) ventral exoskeleton of mature female (VM), and (4) dorsolateral exoskeleton of mature female (DM). Polyvinyl chloride tubes (artificial females; AF) were coated with each extract and the behavior displayed by sexually mature males in contact with the AF was recorded and classified as follows: 0 = no response; 1 = contact; 2 = pushing; and 3 = prolonged contact (≥10 s). To test the hypothesis that the extracts collected from the ventral portion of the abdomen exoskeleton have a higher effect on the behavior of males than the extracts collected from the dorsolateral portion of the abdomen exoskeleton, the experiment was divided into two bioassays: Bioassay I (VI vs. DI) and Bioassay II (VM vs. DM). In each bioassay, all experimental treatments were significantly different (p > 0.05) from the CTL group (AF coated with hexane). Notably, the pushing behavior was significantly higher (p < 0.05) in the VI treatment compared to the CTL and DI treatment. These results provide evidence of the existence of contact female sex pheromones with sexual recognition function located primarily in the ventral portion of the abdomen exoskeleton of P. vannamei.
Changes in Rumen Microbiology and Metabolism of Tibetan Sheep with Different Lys/Met Ratios in Low-Protein Diets
Authors: Zhang Fengshuo , Zhang Yu , He Tingli , Ji Qiurong , Hou Shengzhen , Gui Linsheng
Published: May 2024

In ruminants, supplementing appropriate amounts of amino acids improves growth, feed utilization efficiency, and productivity. This study aimed to assess the effects of different Lys/Met ratios on the ruminal microbial community and the metabolic profiling in Tibetan sheep using 16S rDNA sequencing and non-target metabolomics. Ninety-two-month-old Tibetan rams (initial weight = 15.37 ± 0.92 kg) were divided into three groups and fed lysine/methionine (Lys/Met) of 1:1 (LP-L), 2:1 (LP-M), and 3:1 (LP-H) in low-protein diet, respectively. Results: The T-AOC, GSH-Px, and SOD were significantly higher in the LP-L group than in LP-H and LP-M groups (p < 0.05). Cellulase activity was significantly higher in the LP-L group than in the LP-H group (p < 0.05). In the fermentation parameters, acetic acid concentration was significantly higher in the LP-L group than in the LP-H group (p < 0.05). Microbial sequencing analysis showed that Ace and Chao1 indicators were significantly higher in LP-L than in LP-H and LP-M (p < 0.05). At the genus level, the abundance of Rikenellaceae RC9 gut group flora and Succiniclasticum were significantly higher in LP-L than in LP-M group (p < 0.05). Non-target metabolomics analyses revealed that the levels of phosphoric acid, pyrocatechol, hydrocinnamic acid, banzamide, l-gulono-1,4-lactone, cis-jasmone, Val-Asp-Arg, and tropinone content were higher in LP-L. However, l-citrulline and purine levels were lower in the LP-L group than in the LP-M and LP-H groups. Banzamide, cis-jasmone, and Val-Asp-Arg contents were positively correlated with the phenotypic contents, including T-AOC, SOD, and cellulase. Phosphoric acid content was positively correlated with cellulase and lipase activities. In conclusion, the Met/Lys ratio of 1:1 in low-protein diets showed superior antioxidant status and cellulase activity in the rumen by modulating the microbiota and metabolism of Tibetan sheep.
SVHRSP Alleviates Age-Related Cognitive Deficiency by Reducing Oxidative Stress and Neuroinflammation
Authors: Wang Yingzi , Wang Zhenhua , Guo Songyu , Li Qifa , Kong Yue , Sui Aoran , Ma Jianmei , Lu Li , Zhao Jie , Li Shao
Published: May 2024

Background: Our previous studies have shown that scorpion venom heat-resistant synthesized peptide (SVHRSP) induces a significant extension in lifespan and improvements in age-related physiological functions in worms. However, the mechanism underlying the potential anti-aging effects of SVHRSP in mammals remains elusive. Methods: Following SVHRSP treatment in senescence-accelerated mouse resistant 1 (SAMR1) or senescence-accelerated mouse prone 8 (SAMP8) mice, behavioral tests were conducted and brain tissues were collected for morphological analysis, electrophysiology experiments, flow cytometry, and protein or gene expression. The human neuroblastoma cell line (SH-SY5Y) was subjected to H2O2 treatment in cell experiments, aiming to establish a cytotoxic model that mimics cellular senescence. This model was utilized to investigate the regulatory mechanisms underlying oxidative stress and neuroinflammation associated with age-related cognitive impairment mediated by SVHRSP. Results: SVHRSP significantly ameliorated age-related cognitive decline, enhanced long-term potentiation, restored synaptic loss, and upregulated the expression of synaptic proteins, therefore indicating an improvement in synaptic plasticity. Moreover, SVHRSP demonstrated a decline in senescent markers, including SA-β-gal enzyme activity, P16, P21, SIRT1, and cell cycle arrest. The underlying mechanisms involve an upregulation of antioxidant enzyme activity and a reduction in oxidative stress-induced damage. Furthermore, SVHRSP regulated the nucleoplasmic distribution of NRF2 through the SIRT1-P53 pathway. Further investigation indicated a reduction in the expression of proinflammatory factors in the brain after SVHRSP treatment. SVHRSP attenuated neuroinflammation by regulating the NF-κB nucleoplasmic distribution and inhibiting microglial and astrocytic activation through the SIRT1-NF-κB pathway. Additionally, SVHRSP significantly augmented Nissl body count while suppressing neuronal loss. Conclusion: SVHRSP could remarkably improve cognitive deficiency by inhibiting oxidative stress and neuroinflammation, thus representing an effective strategy to improve brain health.
The Effect of Tongkat Ali Supplementation on Body Composition in Exercise-Trained Males and Females
Authors: Antonio Jose , Evans Cassandra , Pereira Flavia , Thakkar Hena , Miriyala Viraaj , Rocanelli Robert , Castillo Cesar , Andal Alyana , Rojas Jose , Santana Juan Carlos , Jiannine Lia , Tartar Jaime , Curtis Jason
Published: May 2024

Background: It has been suggested that supplementation with Tongkat Ali may affect testosterone concentrations; moreover, whether this influences body composition is unclear. Thus, the purpose of this investigation was to determine if four weeks of Tongkat Ali (400 mg daily dose) supplementation affected body composition and salivary free testosterone concentrations. Methods: Thirty-three exercise-trained males (n = 19) and females (n = 14) volunteered for this investigation (mean ± standard deviation: age 33.1 ± 13.0 years; height 171.1 ± 11.3 cm; body mass 77.4 ± 16.8 kg; average total years of training 13.9 ± 13.2; average hours of resistance training/week 4.2 ± 2.5; average hours of aerobic exercise/week 3.4 ± 2.8; average hours of other exercise/week 1.1 ± 2.0). Research participants were pre- and post-tested for body composition (InBody 270), Profile of Mood States (POMS), handgrip strength, and sleep (Pittsburgh Sleep Quality Index [PSQI]). In a subset of the research participants, saliva samples were collected and analyzed for cortisol and free testosterone. The research participants consumed 400 mg of Tongkat Ali or a placebo (rice flour) daily for four weeks. Results: There were no between-group differences (p > 0.05) for body composition (delta score, mean ± SD: lean body mass kg-treatment −0.5 ± 1.9, placebo −0.4 ± 0.7; fat mass kg-treatment −0.5 ± 1.6, placebo 0.3 ± 1.0, % fat treatment −0.1 ± 1.4, placebo 0.5 ± 1.1). Moreover, there were no differences between groups for any additional assessments (e.g., handgrip strength, mood, sleep, and salivary free testosterone and cortisol). Conclusions: Four weeks of supplementation with Tongkat Ali in exercise-trained males and females does not affect body composition, mood, sleep, vigilant attention, handgrip strength, cortisol, and free testosterone.
Exploring the Feasibility of Vision-Based Non-Contact Oxygen Saturation Estimation: Considering Critical Color Components and Individual Differences
Authors: Seong Hyeon Ah , Seok Chae Lin , Lee Eui Chul
Published: May 2024

The blood oxygen saturation, which indicates the ratio of oxygenated hemoglobin to total hemoglobin in the blood, is closely related to one’s health status. Oxygen saturation is typically measured using a pulse oximeter. However, this method can cause skin irritation, and in situations where there is a risk of infectious diseases, the use of such contact-based oxygen saturation measurement devices can increase the risk of infection. Therefore, recently, methods for estimating oxygen saturation using facial or hand images have been proposed. In this paper, we propose a method for estimating oxygen saturation from facial images based on a convolutional neural network (CNN). Particularly, instead of arbitrarily calculating the AC and DC components, which are essential for measuring oxygen saturation, we directly utilized signals obtained from facial images to train the model and predict oxygen saturation. Moreover, to account for the time-consuming nature of accurately measuring oxygen saturation, we diversified the model inputs. As a result, for inputs of 10 s, the Pearson correlation coefficient was calculated as 0.570, the mean absolute error was 1.755%, the root mean square error was 2.284%, and the intraclass correlation coefficient was 0.574. For inputs of 20 s, these metrics were calculated as 0.630, 1.720%, 2.219%, and 0.681, respectively. For inputs of 30 s, they were calculated as 0.663, 2.142%, 2.612%, and 0.646, respectively. This confirms that it is possible to estimate oxygen saturation without calculating the AC and DC components, which heavily influence the prediction results. Furthermore, we analyzed how the trained model predicted oxygen saturation through ‘SHapley Additive exPlanations’ and found significant variations in the feature contributions among participants. This indicates that, for more accurate predictions of oxygen saturation, it may be necessary to individually select appropriate color channels for each participant.
X-ray Line-Intensity Ratios in Neon-like Xenon: Significantly Reducing the Discrepancy between Measurements and Simulations
Authors: Huang Shihan , Tang Zhiming , Yang Yang , Zhang Hongming , Tian Ziqiang , Ma Shaokun , Li Jinyu , Zeng Chao , Ji Huajian , Yao Ke , Zou Yaming
Published: May 2024

The X-ray spectra of L-shell transitions in Neon-like Xenon ion (Xe44+) have been precisely measured at the Shanghai Electron-Beam Ion Trap using a high-resolution crystal spectrometer. Focusing on the line-intensity ratio of the 3F {2p6-(2p51/23s1/2)J=1} and 3D {2p6-(2p53/23d5/2)J=1} lines (3F/3D), our measurements have achieved remarkable precision improvements over the previous studies. These spectra have been simulated using the collisional-radiative model (CRM) within the Flexible Atomic Code, showing good agreement with the measurements. The previously reported discrepancies, approximately ranging from 10% to 20%, have been significantly reduced in this work to below 1.4% for electron-beam energies exceeding 6 keV and to around 7% for lower energies. Furthermore, our analysis of population fluxes of the involved levels reveals a very high sensitivity of the 3F line to radiation cascades. This suggests that the current CRM, which conventionally excludes interionic population transfer processes, may underestimate the population of the upper level of the 3F line and the cascade-related higher levels, thus explaining the remaining discrepancies. These findings provide a solid foundation for further minimizing these discrepancies and are crucial for understanding the atomic structure and plasma model of these ions.
Study on Rock Interface Stability in the Heat Exchange Channel of the Horizontal Section of U-Shaped Wells in Hot Dry Rock
Authors: Xue Yafei , Xiong Bo , Wang Shejiao , Fang Chaohe , Mo Shaoyuan , Xin Fudong , Xian Yuxi
Published: May 2024

Enhanced Geothermal Systems (EGS) represent a promising direction for sustainable energy development, yet their efficiency and feasibility often suffer due to suboptimal heat extraction methods and interface instability in U-shaped wells. This study introduces an innovative volume encapsulation technology that aims to address these challenges. The proposed technology employs a combination of hydraulic fracturing and acidification to prepare the rock interface, followed by encapsulation using high-temperature liquid metal. Low-melting-point alloys are utilized as a heat exchange medium between the horizontal sections of the wells. This study meticulously analyzes the impact of formation stress, thermal shock stress, and liquid metal properties on rock interface stability. Advanced simulation tools and experimental setups were used to test the encapsulation process under various conditions. The application of liquid metal encapsulation demonstrated significant improvements in energy conversion efficiency and rock interface stability. In conditions simulating a dry and hot rock reservoir at depths up to 3000 m and temperature gradients reaching 2200 °C/m, the adjusted depth of horizontal sections and increased pumping pressure contributed to maintaining interface stability. The established failure criteria provide a robust theoretical foundation for the encapsulation process. Volume encapsulation technology using liquid metal not only enhances the operational efficiency of EGS but also stabilizes the rock interface, thereby increasing the feasibility of continuous geothermal energy extraction. This study offers valuable theoretical insights and practical guidance for future research and applications in geothermal energy technologies, creating new pathways for the efficient exploitation of geothermal resources.
Comparative Analysis of Energy Efficiency between Battery Electric Buses and Modular Autonomous Vehicles
Authors: Oargă Ioan-Tudor , Prunean Gabriel , Varga Bogdan Ovidiu , Moldovanu Dan , Micu Dan Doru
Published: May 2024

This paper presents the initial steps taken in analysing the benefits of connected autonomous vehicles (CAVs), especially Modular Autonomous Vehicles (MAVs), in search of sustainable solutions for reducing energy consumption per passenger in air transport. For this particular case, a Mobility-as-a-Service (MaaS) solution is proposed, correlating airside transport with landside transport, as an urban mobility alternative. To better understand the benefits of the proposal, this paper studies the impact on energy consumption conceptual differences between a conventional public transport fleet using Battery Electric Buses (BEBs) and an MAV fleet. Simulations for simple public transport tasks are performed to highlight the advantages of the modular vehicle concept, in which routes are assigned dynamically based on the requested passenger carrying capacity and travel distance, aiming to optimize the efficiency of the entire system. With a proven reduction in energy consumption due to better use of available passenger capacity and reduced travel times in which the vehicle is driving with a number of passengers less than half of its capacity, the concept can be addressed further in developing a predictive system that processes public transport data and delivers an optimized schedule for the entire fleet. The main goal being to improve overall operational efficiency and total cost of ownership, the second part of the paper studies the impact of weight distribution on efficiency parameters such as energy consumption, range, and overall performance of an electric bus. The impact on dynamic elements such as acceleration, braking, and cornering performance is analyzed, to assess the viability and safety of all types of electric bus operations.
Ecosystem of Aviation Maintenance: Transition from Aircraft Health Monitoring to Health Management Based on IoT and AI Synergy
Authors: Kabashkin Igor , Perekrestov Vladimir
Published: May 2024

This paper presents an in-depth exploration of the transformative impact of integrating the Internet of Things (IoT), cloud computing, and artificial intelligence (AI) within the domain of aviation maintenance. It articulates the transition from conventional health monitoring practices to a more advanced, comprehensive health management approach, leveraging these modern technologies. This paper emphasizes the pivotal shift from reactive maintenance strategies to proactive and predictive maintenance paradigms, facilitated by the real-time data collection capabilities of IoT devices and the analytical prowess of AI. This transition not only enhances the safety and reliability of flight operations but also optimizes maintenance procedures, thereby reducing operational costs and improving efficiency. This paper meticulously outlines the implementation challenges, including technological integration, regulatory compliance, and security concerns, while proposing a future research agenda to address these issues and further harness the potential of these technologies in revolutionizing aviation maintenance.
Birthplace and Birthdate Effect during Talent Process in Professional Soccer Academy Players
Authors: Hernandez-Simal Lander , Calleja-González Julio , Lorenzo Calvo Alberto , Aurrekoetxea-Casaus Maite
Published: May 2024

The main objective of this study was to detect, from among a set of innate, acquired, and contextual factors, those variables that are ascribed to players ultimately promoted to the professional team of a Spanish league club during the earlier selection and development phases. The data were presented in frequencies and correlations and by means of a classificatory cluster model. The variables used for the analyses included date of birth, birthplace density, player position, laterality, academy entry stage, international participation, and debutant status. These variables were related to the talent selection and promotion phases (i.e., academy entry stage and player debut). A dataset of information on 1411 players from the last 30 seasons of the Athletic Club de Bilbao (1993–2021) was used. Regarding the results, first, there was an over-representation of players with respect to their Q1 birth date and K5 density quintile in the selection phase; however, once players joined the academy, their chances of promotion (debut) fell for players in the Q4 birth date and K3 density quintiles. Second, there was a significant correlation between players’ debut and the stage of incorporation (p < 0.01; V = 0.46) and internationalisation (p < 0.01; V = 0.5). Finally, the birthplace density and laterality variables converged as classificatory features of the players.
Optimization of Composite Material Repair Patch Shape Based on Strength Analysis
Authors: Xing Ruishan , Wang Fan , Yang Yang , Li Gang
Published: May 2024

With the increasing use of composite materials in aircraft structures and the extension of their service life, the selection of repair patch shapes for composite material damage repair has become a significant engineering concern. The ultimate strength of the repaired structure is among the main evaluation criteria for selecting optimal patch shapes. In this study, strength analysis is conducted along with the use of the Digital Image Correlation (DIC) method to assess the quality of the repaired components, making the evaluation method more rational. Early studies often focused on simplified models, which diverged significantly from the practicalities of maintenance engineering in civil aviation. Therefore, it is essential to research full-scale composite material repair patches, as this will provide a more reliable basis for the optimal selection of patch shapes in composite material maintenance engineering for civil aircraft.
Reassessing Gout Management through the Lens of Gut Microbiota
Authors: Demarquoy Jean , Dehmej Oumaima
Published: May 2024

Gout, recognized as the most common form of inflammatory arthritis, arises from the accumulation of uric acid crystals, leading to intense pain, particularly in the big toe. This condition has traditionally been associated with the overproduction or reduced clearance of uric acid. Recent studies, however, have underscored the significant role of the gut microbiota in uric acid metabolism, impacting both its production and elimination. This emerging understanding suggests that maintaining gut health could offer innovative approaches to treating gout, complementing traditional dietary and pharmacological interventions. It highlights the potential of probiotics or microbiome-based therapies, indicating a future where treatments are tailored to an individual’s microbiome. This offers a fresh perspective on gout management and underscores the broader influence of the microbiota on health and disease.
Relations between High Anticyclonic Atmospheric Types and Summer Season Temperature in Bulgaria
Authors: Pophristov Vulcho , Nikolova Nina , Matev Simeon , Gera Martin
Published: May 2024

The atmospheric circulation, not only near the surface but also at high altitudes, is probably the main factor determining the weather and climate of a given area, along with its latitude, altitude, the shape of the relief of the area and its surroundings, and the proximity of water basins of different sizes. The main objective of this study is to investigate the relationship between anticyclonic circulation types in the middle troposphere at the 500 hPa level and the seasonal summer temperature over the region of the central Balkan Peninsula, particularly Bulgaria. A previously compiled classification of atmospheric circulation is used, and the frequencies of the circulation types are correlated with the mean seasonal (monthly) temperature, where the extreme seasons and months are defined as the 10th percentile for cold summer seasons and months and the 90th percentile for warm ones. A positive and statistically significant correlation was found for the anticyclones located southwest of Bulgaria and a negative one for those located southeast of it. A comparison between the last two 30-year climatological periods (1961–1990 and 1991–2020) was also made, and an irrefutable decrease in the number of cold summer seasons from 257 to just 17 was found in the last 30 years, respectively, as well as a rapid increase in the number of extreme warm summer seasons from 26 to 263, encompassing all 15 meteorological stations studied.
Poptosis or Peptide-Induced Transmembrane Pore Formation: A Novel Way to Kill Cancer Cells without Affecting Normal Cells
Authors: Pincus Matthew R. , Silberstein Miriam , Zohar Nitzan , Sarafraz-Yazdi Ehsan , Bowne Wilbur B.
Published: May 2024

Recent advances in cancer treatment like personalized chemotherapy and immunotherapy are aimed at tumors that meet certain specifications. In this review, we describe a new approach to general cancer treatment, termed peptide-induced poptosis, in which specific peptides, e.g., PNC-27 and its shorter analogue, PNC-28, that contain the segment of the p53 transactivating 12–26 domain that bind to HDM-2 in its 1–109 domain, bind to HDM-2 in the membranes of cancer cells, resulting in transmembrane pore formation and the rapid extrusion of cancer cell contents, i.e., tumor cell necrosis. These peptides cause tumor cell necrosis of a wide variety of solid tissue and hematopoietic tumors but have no effect on the viability and growth of normal cells since they express at most low levels of membrane-bound HDM-2. They have been found to successfully treat a highly metastatic pancreatic tumor as well as stem-cell-enriched human acute myelogenous leukemias in nude mice, with no evidence of off-target effects. These peptides also are cytotoxic to chemotherapy-resistant cancers and to primary tumors. We performed high-resolution scanning immuno-electron microscopy and visualized the pores in cancer cells induced by PNC-27. This peptide forms 1:1 complexes with HDM-2 in a temperature-independent step, followed by dimerization of these complexes to form transmembrane channels in a highly temperature-dependent step parallel to the mode of action of other membranolytic but less specific agents like streptolysin. These peptides therefore may be effective as general anti-cancer agents.
Situ-Oracle: A Learning-Based Situation Analysis Framework for Blockchain-Based IoT Systems
Authors: Bian Hongyi , Zhang Wensheng , Chang Carl K.
Published: May 2024

The decentralized nature of blockchain enables data traceability, transparency, and immutability as complementary security features to the existing Internet of Things (IoT) systems. These Blockchain-based IoT (BIoT) systems aim to mitigate security risks such as malicious control, data leakage, and dishonesty often found in traditional cloud-based, vendor-specific IoT networks. As we steadily advance into the era of situation-aware IoT, the use of machine learning (ML) techniques has become essential for synthesizing situations based on sensory contexts. However, the challenge to integrate learning-based situation awareness with BIoT systems restricts the full potential of such integration. This is primarily due to the conflicts between the deterministic nature of smart contracts and the non-deterministic nature of machine learning, as well as the high costs of conducting machine learning on blockchain. To address the challenge, we propose a framework named Situ-Oracle. With the framework, a computation oracle of the blockchain ecosystem is leveraged to provide situation analysis as a service, based on Recurrent Neural Network (RNN)-based learning models tailored for the Situ model, and specifically designed smart contracts are deployed as intermediary communication channels between the IoT devices and the computation oracle. We used smart homes as a case study to demonstrate the framework design. Subsequently, system-wide evaluations were conducted over a physically constructed BIoT system. The results indicate that the proposed framework achieves better situation analysis accuracy (above 95%) and improves gas consumption as well as network throughput and latency when compared to baseline systems (on-chain learning or off-chain model verification). Overall, the paper presents a promising approach for improving situation analysis for BIoT systems, with potential applications in various domains such as smart homes, healthcare, and industrial automation.
Utility of Combining Transcranial Sonography and MIBG Myocardial Scintigraphy to Evaluate Substantia Nigra in Patients with Parkinson’s Disease
Authors: Mizukami Heisuke , Shiraishi Makoto , Hino Sakae , Kaburagi Mayumi , Matsumoto Hirofumi , Hagiwara Yuta , Yamano Yoshihisa
Published: May 2024

The utility of transcranial sonography (TCS) remains unclarified for the auxiliary diagnosis of Parkinson’s disease (PD). We investigated iodine-123 metaiodobenzylguanidine (MIBG) and TCS during the examination and diagnosis of high-signal-intensity substantia nigra lesion (HSI-SNL) incidence in PD patients previously diagnosed with dopamine transporter scintigraphy (DAT). The subjects were 67 patients with definitively diagnosed PD after DAT evaluation. Patients with midbrain substantia nigra visible during TCS who previously underwent MIBG were analyzed. The SN+ group comprised patients with extensive pathological HSI-SNL of Okawa class III/IV observed during TCS. The MIBG+ group comprised patients with a heart-to-mediastinum ratio of ≤2.2 during MIBG. TCS was performed to divide patients into the SN+ and SN− groups, and patient characteristics and MIBG findings were compared between the groups. PD was definitively diagnosed in 67 patients, among whom midbrain was visualized during TCS in 43 (64.1%) patients and pathological HSI-SNL was observed in 24 (35.8%). The MIBG findings were normal in six patients (27.3%) with HSI-SNL, and abnormal in seven (63.6%) without HSI-SNL. No significant differences were noted by Okawa classification in clinical characteristics based on the presence or absence of HSI-SNL. Multiple patients with normal findings during MIBG may have HSI-SNL. Thus, confirmatory imaging of HSI-SNL with TCS may be useful for diagnosis.
Familiarity Determines Whether Accent Affects Attitudes and Behaviors of the Listener
Authors: Cheng Zenghu , She Yugui , Fu Junjun , Xu Wenming
Published: May 2024

Previous research found that accents cause the listener to exhibit prejudice toward the speaker. The present study tested whether the familiarity of the listener and speaker moderated this effect. Study 1 tested this question in a simulated recruit scenario and found that participants were less likely to recruit candidates with an accent, but this effect existed only when the candidate was a stranger to the interviewer, not when the candidate was an acquaintance. Study 2 retested this question in a scenario of talking one-on-one and also found that the effect of accent existed only when they were strangers, not when they were acquaintances. Both studies suggested that the effect of accent on the attitude and behavior of the listener vanished when the speaker and listener were familiar with each other. This work offers insights for understanding the effect of accent on social interaction from the perspective of the familiarity of the speaker and listener and reveals the moderated role of familiarity in the dynamic of the effect of accent.
Nature-Based Solutions and Climate Resilience: A Bibliographic Perspective through Science Mapping Analysis
Authors: Vazin Farnoosh , Chan Daniel W. M. , Hanaee Toktam , Sarvari Hadi
Published: May 2024

Currently, urban areas are confronting considerable challenges stemming from climate change. These challenges possess the potential to have profound implications for the well-being of residents, their means of making a living, and their own assets. Consequently, it is imperative to identify effective solutions that mitigate these effects on the urban environment. Nature-based solutions (NBSs), involving the utilization of natural resources and ecosystem services to alleviate the effects of climate change, have the potential of enhancing our capacity to develop cities that are more resistant to these challenges. To this end, this paper aims to extract some bibliographic data from available research articles on NBSs and climate resilience sought from the common search engines of Scopus, Web of Science, and Lens Base, and then the RStudio version 2022.12.00+353, VoSviewer version 1.6.20, and Biblioshiny-Bibliometrics version 2017 software tools were adopted to collate and analyze the literature data through science mapping analysis. In doing so, it was advocated that these two topics have not been extensively studied in their relationship to each other and that there is a large extent of existing knowledge gaps, the filling of which can foster the development of these ideas and thus help develop our cities in combating against climate change. Exploring the effectiveness of NBSs in boosting climate resilience is a critical research gap. More empirical studies are envisaged to assess the scalability and transferability of those effective NBSs in various regions or countries. Another research gap exists in comprehending the long-term effects on ecosystem services and community welfare. Research on socio-economic advantages, trade-offs, and unintended outcomes is essential for making well-informed decisions when applying various NBSs worldwide.
Gamification Approaches and Assessment Methodologies for Occupants’ Energy Behavior Change in Buildings: A Systematic Review
Authors: Li Wen-Ting , Iuorio Ornella , Fang Han , Mak Michele Win Tai
Published: May 2024

With the trend of achieving both energy efficiency in buildings and occupants’ comfort, gamification strategies have started to be developed and applied as incentive mechanisms to increase social interaction and facilitate human energy behavior transformation. In this article, 306 published papers are reviewed, and 21 studies are identified to determine the challenges and potential for the development of gamification strategies to improve building energy efficiency. Specifically, this work reviews the implementation techniques of gamification and methods to assess the impact of gamification mechanisms on human energy behavior changes. This analysis demonstrates that, firstly, the choice of an optimal gamification implementation method should be inherently attuned to the distinct characteristics of the building type and its occupants. Secondly, it is imperative to strike a judicious balance between extrinsic and intrinsic motivations, in which customization of gamification design elements are based on users’ unique personality traits and preferences, to properly tailor gamification mechanisms. Thirdly, integrating a fusion of quantification of energy savings and qualitative interpretation of user behaviors to improve the energy efficiency in buildings is essential for a more holistic understanding of the impact of gamification on users’ energy-related behavior change. The findings indicate that gamification techniques can enable the effective reduction of energy consumption in buildings.
Combined Regional Approach of Talimogene laherparepvec and Radiotherapy in the Treatment of Advanced Melanoma
Authors: Tam Andrew , Ladbury Colton , Kassardjian Ari , Modi Badri , McGee Heather , Melstrom Laleh , Margolin Kim , Xing Yan , Amini Arya
Published: May 2024

Talimogene laherparepvec (TVEC) is a genetically modified oncolytic herpes simplex virus (HSV-1) that is used for the intralesional treatment of advanced or metastatic melanoma. Given that TVEC produces the granulocyte–macrophage colony-stimulating factor (GM-CSF), recent reports have suggested that radiation treatment (RT) given in conjunction with TVEC may provide synergistic immune activation at the site, and possibly systemically. However, studies on combining RT with TVEC remain limited. We conducted a retrospective review of melanoma patients from a single cancer center who received TVEC and RT in the same region of the body and compared them to patients who received TVEC with RT at another site (other than the site of TVEC injection). Between January 2015 and September 2022, we identified twenty patients who were treated with TVEC and RT; fourteen patients received TVEC and RT in the same region, and six had treatments in separate regions. Regions were determined at the time of analysis and were based on anatomic sites (such as arm, leg, torso, etc.). Kaplan–Meier analysis of progression-free survival (PFS), analyses of time to distant metastasis (DM), overall survival (OS), and locoregional control (LRC), and the corresponding log-rank test were performed. With a median follow-up of 10.5 months [mos] (range 1.0–58.7 mos), we found an improvement in PFS with TVEC and RT in the same region compared to different regions, which were 6.4 mos (95% CI, 2.4–NR mos) and 2.8 mos (95% CI, 0.7–4.4 mos), respectively; p = 0.005. There was also a significant improvement in DM when TVEC and RT were used in the same region compared to different regions: 13.8 mos (95% CI, 4.6–NR mos) and 2.8 mos (95% CI, 0.7–4.4 mos), respectively (p = 0.001). However, we found no difference in overall survival (OS) between patients who had TVEC and RT in the same region (19.0 mos, 95% confidence interval [CI], 4.1–not reached [NR] mos) and those who received treatments in different regions (18.5 mos, 95% CI, 1.0–NR mos); p = 0.366. There was no statistically significant improvement in locoregional control (LRC) in patients who had TVEC and RT in the same region was 26.0 mos (95% CI, 6.4–26.0 mos) compared to patients who received TVEC and RT in different regions (4.4 mos) (95% CI, 0.7–NR mos) (p = 0.115). No grade 3 or higher toxicities were documented in either group. Overall, there were improvements in PFS and DM when TVEC and RT were delivered to the same region of the body compared to when they were used in different regions. However, we did not find a significant difference in locoregional recurrence or OS. Future studies are needed to assess the sequence and timing of combining RT and TVEC to potentially enhance the immune response both locally and distantly.
Therapeutic Patterns and Clinical Outcomes in Limited Disease Small Cell Lung Cancer: A Decade of Analysis at a Tertiary Cancer Center
Authors: Ziegler David Alexander , Cleve Cecilia Christiane , Ziegler Sonia , Schirmer Markus Anton , Fischer Laura Anna , Bohnenberger Hanibal , Overbeck Tobias Raphael , Braulke Friederike , Hammerstein-Equord Alexander von , Leu Martin , Guhlich Manuel , Dröge Leif Hendrik , Rieken Stefan , Rittmeyer Achim , El Shafie Rami A.
Published: May 2024

In this study, we investigated the outcomes and factors influencing treatment efficacy in 93 patients with limited disease small cell lung cancer (LD-SCLC), with a median age of 64 years. We focused on the impact of chemotherapy regimens, prophylactic cranial irradiation (PCI), and patient-related variables. The median follow-up for OS was 17.3 months. We observed a statistically significant difference in PFS between LD-SCLC patients treated with cisplatin and etoposide (EP) and those treated with carboplatin and etoposide (CP) (PFS: EP 13.63 months vs. CP 6.54 months, p < 0.01). Patients treated with EP had better overall survival (OS) than CP-treated patients (OS: EP 26.9 months vs. CP 16.16 months, p < 0.01). Concomitant chemotherapy was associated with improved PFS (p = 0.003) and OS (p = 0.002). Patients receiving PCI showed superior OS (p = 0.05) and a trend towards improved PFS (p = 0.057). Female gender was associated with better OS (p = 0.025). Most patients had an ECOG performance status of 0 (71%). This real-world study underscores the importance of multidisciplinary LD-SCLC management, emphasizing the roles of chemotherapy, radiotherapy, and PCI. These findings inform personalized treatment strategies and emphasize the need for prospective trials to validate these results and optimize LD-SCLC treatment.
Integrating Ataxia Evaluation into Tumor-Induced Hearing Loss Model to Comprehensively Study NF2-Related Schwannomatosis
Authors: Lu Simeng , Yin Zhenzhen , Chen Jie , Wu Limeng , Sun Yao , Gao Xing , Huang Peigen , Jordan Justin , Plotkin Scott , Xu Lei
Published: May 2024

NF2-related Schwannomatosis (NF2-SWN) is a disease that needs new solutions. The hallmark of NF2-SWN, a dominantly inherited neoplasia syndrome, is bilateral vestibular schwannomas (VSs), which progressively enlarge, leading to sensorineural hearing loss, tinnitus, facial weakness, and pain that translates to social impairment and clinical depression. Standard treatments for growing VSs include surgery and radiation therapy (RT); however, both carry the risk of further nerve damage that can result in deafness and facial palsy. The resultant suffering and debility, in combination with the paucity of therapeutic options, make the effective treatment of NF2-SWN a major unmet medical need. A better understanding of these mechanisms is essential to developing novel therapeutic targets to control tumor growth and improve patients’ quality of life. Previously, we developed the first orthotopic cerebellopontine angle mouse model of VSs, which faithfully mimics tumor-induced hearing loss. In this model, we observed that mice exhibit symptoms of ataxia and vestibular dysfunction. Therefore, we further developed a panel of five tests suitable for the mouse VS model and investigated how tumor growth and treatment affect gait, coordination, and motor function. Using this panel of ataxia tests, we demonstrated that both ataxia and motor function deteriorated concomitantly with tumor progression. We further demonstrated that (i) treatment with anti-VEGF resulted in tumor size reduction, mitigated ataxia, and improved rotarod performance; (ii) treatment with crizotinib stabilized tumor growth and led to improvements in both ataxia and rotarod performance; and (iii) treatment with losartan did not impact tumor growth nor ameliorate ataxia or motor function. Our studies demonstrated that these methods, paired with hearing tests, enable a comprehensive evaluation of tumor-induced neurological deficits and facilitate the assessment of the effectiveness of novel therapeutics to improve NF2 treatments.