bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancing Semen Evaluation Using Lipidomics
Authors: Evans Holly C. , Dinh Thu T. N. , Hardcastle Madison L. , Gilmore Alicia A. , Ugur Muhammet R. , Hitit Mustafa , Jousan Frank Dean , Nicodemus Molly C. , Memili Erdogan
Published: April 2021

Developing a deeper understanding of biological components of sperm is essential to improving cryopreservation techniques and reproductive technologies. To fully ascertain the functional determinants of fertility, lipidomic methods have come to the forefront. Lipidomics is the study of the lipid profile (lipidome) within a cell, tissue, or organism and provides a quantitative analysis of the lipid content in that sample. Sperm cells are composed of various lipids, each with their unique contribution to the overall function of the cell. Lipidomics has already been used to find new and exciting information regarding the fatty acid content of sperm cells from different species. While the applications of lipidomics are rapidly evolving, gaps in the knowledge base remain unresolved. Current limitations of lipidomics studies include the number of available samples to analyze and the total amount of cells within those samples needed to detect changes in the lipid profiles across different subjects. The information obtained through lipidomics research is essential to systems and cellular biology. This review provides a concise analysis of the most recent developments in lipidomic research. This scientific resource is important because these developments can be used to not only combat the reproductive challenges faced when using cryopreserved semen and artificial reproductive technologies in livestock such as cattle, but also other mammals, such as humans or endangered species.
Investigation of a 2-DOF Active Magnetic Bearing Actuator for Unmanned Underwater Vehicle Thruster Application
Authors: Ahad Muhammad Abdul , Ahmad Sarvat M.
Published: April 2021

In this work, a novel application of Active Magnetic Bearing (AMB) is proposed to integrate AMB in the Magnetically Coupled Thruster (MCT) assembly for underwater application. In this study, a 2-Degree-Of-Freedom (DOF) AMB is developed and investigated for the MCT of an Unmanned Underwater Vehicle (UUV). The paper presents the detailed electro-mechanical modeling of the in-house developed AMB system. The intractable problem of rotor suspension and rotation with opposing pairs of electromagnets is considered. A Linear Quadratic Gaussian (LQG) controller is designed and analyzed in the frequency domain for the stabilization of the open-loop unstable AMB for MCT. The performance specifications of the controller, such as reference tracking and disturbance rejection are achieved and evaluated through real-time implementation of the controller. The compensator also performed reasonably well during the dynamic operations, i.e., when the rotor-propeller assembly was spun at 1500 rpm. This rotor speed is needed to generate a thrust of 40–45 N and up to 1 m/s forward velocity, which is necessary to propel the UUV under consideration. By deploying AMB in MCT assembly, it is anticipated that problems associated with the conventional directly coupled thruster operating in harsh underwater environment, such as water ingress into electronics compartment, rusting, lubrication, and vibrations would be eliminated.
Yield Enhancement and Better Micronutrients Uptake in Tomato Fruit through Potassium Humate Combined with Micronutrients Mixture
Authors: Rahi Ashfaq Ahmad , Anjum Muhammad Akbar , Iqbal Mirza Javed , Ahmad Ali Saleha , Marfo Theodore Danso , Fahad Shah , Danish Subhan , Datta Rahul
Published: April 2021

The deficiency of micronutrients and low organic matter (OM) status of soils are major hurdles in the optimum crop yield achievements. Humic substances can play an imperative role in the micronutrient bioavailability and improved crop yield by ameliorating the physicochemical soil properties. consumed as fresh food and in processed form. However, its susceptibility towards micronutrient deficiency causes a significant reduction in yield. That is why a current study was done to examine the influence of different potassium humate (KH) levels, i.e., 0, 5, 10, and 15 kg ha−1 with micronutrients mixture (MC), i.e., 0, 50 (Zn = 2.50, B = 0.75, Fe = 2.50 kg ha−1), 75 (Zn = 3.75, B = 1.125, Fe = 3.75 kg ha−1) and 100% (Zn = 5.0, B = 1.5, Fe = 5.0 kg ha−1) on the yield, biomass, harvesting index and uptake of the micronutrients in tomato. The results showed that increasing the level of K-humate significantly improved the yield (7.4 and 7.17%), total biomass (6.38 and 6.59%), and harvesting the index (0.46 and 0.76%) of tomato. It also enhanced Fe uptake but did not affect B and Zn concentrations in the tomato fruit at 100% MC + 15 kg ha−1 KH over control in the autumn and spring seasons. In conclusion, both levels of K-humate 10 and 15 kg ha−1 and MC 75 and 100% effectively enhance the yield, biomass, harvesting the index, and micronutrients (B, Zn, and Fe) uptake in tomatoes.
A Heterogeneous Graph Enhanced LSTM Network for Hog Price Prediction Using Online Discussion
Authors: Ye Kai , Piao Yangheran , Zhao Kun , Cui Xiaohui
Published: April 2021

Forecasting the prices of hogs has always been a popular field of research. Such information has played an essential role in decision-making for farmers, consumers, corporations, and governments. It is hard to predict hog prices because too many factors can influence them. Some of the factors are easy to quantify, but some are not. Capturing the characteristics behind the price data is also tricky considering their non-linear and non-stationary nature. To address these difficulties, we propose Heterogeneous Graph-enhanced LSTM (HGLTSM), which is a method that predicts weekly hog price. In this paper, we first extract the historical prices of necessary agricultural products in recent years. Then, we utilize discussions from the online professional community to build heterogeneous graphs. These graphs have rich information of both discussions and the engaged users. Finally, we construct HGLSTM to make the prediction. The experimental results demonstrate that forum discussions are beneficial to hog price prediction. Moreover, our method exhibits a better performance than existing methods.
An Attempt to Enrich Pig Meat with Omega-3 Fatty Acids Using Linseed Oil Ethyl Ester Diet Supplement
Authors: Czyż Katarzyna , Sokoła-Wysoczańska Ewa , Wyrostek Anna , Cholewińska Paulina
Published: April 2021

This study aims to evaluate an effect of pig diet supplementation with ethyl esters derived from linseed oil with a high content of alpha-linolenic acid (ALA) on the fatty acids profile of meat. The study was conducted on Polish Landrace fatteners supplemented for a period of 7 weeks (control and experimental groups of 8 animals each, 16 in total). After this period, loin (Longissimus dorsi) and ham (Biceps femoris) samples were collected for laboratory analysis, including basic composition (fat, protein, ash, dry matter) and fatty acids (FAs) profile. The supplementation caused a significant increase in the level of ALA acid, decrease in the content of saturated fatty acids (SFAs), increase in unsaturated FAs level, and resulting decrease in the ratio of n-6/n-3. The indices of atherogenicity and thrombogenicity were beneficially altered in the experimental groups. It can thus be supposed that meat enriched this way may be considered as an interesting choice for consumers who are aware of the importance of diet consumed.
The Applicability of Species- and Trichothecene-Specific Primers in Monitoring the Fusarium graminearum Species Complex and Its Impact on the Surveillance of Fusarium Head Blight in Winter Wheat in Serbia
Authors: Župunski Vesna , Jevtić Radivoje , Lalošević Mirjana , Mikić Sanja , Orbović Branka
Published: April 2021

Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade.
Foliar Application of Auxin or Cytokinin Can Confer Salinity Stress Tolerance in Vicia faba L.
Authors: Abdel Latef Arafat Abdel Hamed , Akter Ayasha , Tahjib-Ul-Arif Md.
Published: April 2021

Soil salinity severely declines the availability of water and essential minerals to the plants, which hinders growth. The present study evaluates the potential roles of indole-3-acetic acid (IAA) and 6-benzyladenine (BA) for mitigating the adverse effects of soil-salinity in faba bean (Vicia faba L.). Plants were exposed to 150 mM NaCl stress and were sprayed with IAA (1.15 mM) or BA (0.9 mM). Our results revealed that foliar application of IAA or BA improved the growth traits of salinized faba bean due to the increased uptake of K+, Ca2+, and Mg2+ ions, accumulation of free amino acids, soluble sugars, and soluble proteins, and activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. The principal component analysis (PCA) and heatmap clustering indicated that salinity-exposed plants exhibited lower growth and biomass production, which correlated with higher accumulation of Na+ and malondialdehyde. Moreover, electrophoretic patterns of protein showed new bands in IAA- or BA-treated salt-stressed plants, indicating that IAA or BA treatment can reprogram the metabolic processes to confer salinity tolerance. We also found that IAA has a greater capacity to ameliorate the salt stress than BA, although there is no significant difference in yield between these treatments. Finally, these findings can be helpful for a better understanding of IAA- and BA-mediated salt tolerance mechanisms and increasing production of faba bean in saline soils.
Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (Canis lupus familiaris) in Tuscany (Italy)
Authors: Cilia Giovanni , Fratini Filippo , Turchi Barbara , Ebani Valentina Virginia , Turini Luca , Bilei Stefano , Bossù Teresa , De Marchis Maria Laura , Cerri Domenico , Bertelloni Fabrizio
Published: April 2021

Domestic dogs (Canis lupus familiaris) used for wild boar (Sus scrofa) hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti-Leptospira antibodies and the occurrence, antimicrobial resistance, and virulence of Salmonella spp., Yersinia enterocolitica, and Listeria monocytogenes in sera and rectal swabs collected from 42 domestic hunting dogs in the Tuscany region (Italy). Regarding Leptospira, 31 out of 42 serum samples (73.8%) were positive and serogroup Pomona was the most detected (71.4%) at titers between 1:100 and 1:400. Four Salmonella isolates (9.52%) were obtained, all belonging to serotype Infantis; two of them showed antimicrobial resistance to streptomycin, while pipB and sopE presence was assessed in all but one isolate. Concerning Yersinia enterocolitica, seven isolates (16.7%) were obtained, six belonging to biotype 1 and one to biotype 4. Resistance to amoxicillin–clavulanic acid, cephalothin, and ampicillin was detected. Biotype 4 presented three of the virulence genes searched (ystA, ystB, inv), while isolates of biotype 1 showed only one gene. No Listeria monocytogenes was isolated from dog rectal swabs. The results suggest that hunting dogs are exposed to different bacterial zoonotic agents, potentially linked to their work activity, and highlight the possible health risks for humans.
Management Models Applied to the Human-Wolf Conflict in Agro-Forestry-Pastoral Territories of Two Italian Protected Areas and One Spanish Game Area
Authors: Piscopo Nadia , Gentile Leonardo , Scioli Erminia , Eguren Vicente González , Carvajal Urueña Ana Maria , García Tomas Yanes , Alberti Jesús Palacios , Esposito Luigi
Published: April 2021

Our work shows that, despite the persistence of persecutory actions, conservation activity has proved successful for the return of numerous wild mammals to different habitats, including the wolf. The human-wolf conflict is still described in all countries where the wolf is present. This is evidenced by the high number of damages on livestock, and the corpses of wolves found both in protected areas and in those where hunting is permitted. The diagnosis of road accidents, together with poisoning and poaching, are major causes of mortality. Although hunting records the highest percentage of kills in Spain, the demographic stability reported by the censuses suggests that this activity does not have a consistent influence on the Iberian wolf population’s survival. In Italy, where wolf hunting is prohibited, wolf populations are to be increasing. In some Italian situations, wolf attacks on horses seem to cause unwanted damage to foals, but they represent a very precious source of information about the habits of carnivores. A simple management plan would be sufficient to help the coexistence between the productive parts and the ecosystem services ensured by the presence of the wolf. The presence of hybrids is a negative factor.
Influence of Dietary Supplementation with an Amino Acid Mixture on Inflammatory Markers, Immune Status and Serum Proteome in LPS-Challenged Weaned Piglets
Authors: Prates José , Freire João , de Almeida André , Martins Cátia , Ribeiro David , Osório Hugo , Pinho Mário , Lopes Paula , Correia Jorge , Pinto Rui , Costa Teresa , Corrent Etienne , Chalvon-Demersay Tristan
Published: April 2021

In order to investigate the effect of a dietary amino acid mixture supplementation in lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 ± 1.0 kg) newly weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet 1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered LPS (25 μg/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branched-chain amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics. The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two immunoglobulins in the LPS group compared to the control group. In addition, the supplementation with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations compared to the LPS group. Although a proteomics approach did not reveal important alterations in the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked to the immune response, when compared to piglets supplemented with the amino acid mixture. Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are more protected against the detrimental effects of LPS.
Association between THRSP Gene Polymorphism and Fatty Acid Composition in Milk of Dairy Cows
Authors: Polasik Daniel , Golińczak Jacek , Proskura Witold , Terman Arkadiusz , Dybus Andrzej
Published: April 2021

Thyroid hormone-inducible hepatic protein is involved in the de novo synthesis of fatty acids in the lactating mammary gland. Different variants of the gene that encodes this protein may be associated with its different activity. The primary aim of this study was to find polymorphism in the THRSP gene and estimate the relationship between individual genotypes and fatty acid composition in milk. Investigations were carried out on 224 cows represented by two breeds—Jersey (n = 80) and Polish Holstein-Friesian (n = 144). Polymorphism in THRSP was detected by Sanger sequencing; however, genotypes were determined by the PCR-RFLP method. It was shown that the analyzed variant had a significant (p < 0.05) influence on palmitic and stearic fatty acids as well as on fatty acids with a chain length of 14, 16, and 6–16 in Jersey breed and on caproic, palmitic, myristoleic, and palmitoleic fatty acids in H-F. Obtained results indicated that analyzed SNP in bovine THRSP gene (rs42714482) may be considered as a potential marker for fatty acid composition in milk
Risk Factors of Daptomycin-Induced Eosinophilic Pneumonia in a Population with Osteoarticular Infection
Authors: Soldevila-Boixader Laura , Villanueva Bernat , Ulldemolins Marta , Benavent Eva , Padulles Ariadna , Ribera Alba , Borras Irene , Ariza Javier , Murillo Oscar
Published: April 2021

Background: Daptomycin-induced eosinophilic pneumonia (DEP) is a rare but severe adverse effect and the risk factors are unknown. The aim of this study was to determine risk factors for DEP. Methods: A retrospective cohort study was performed at the Bone and Joint Infection Unit of the Hospital Universitari Bellvitge (January 2014–December 2018). To identify risk factors for DEP, cases were divided into two groups: those who developed DEP and those without DEP. Results: Among the whole cohort (n = 229) we identified 11 DEP cases (4.8%) and this percentage almost doubled in the subgroup of patients ≥70 years (8.1%). The risk factors for DEP were age ≥70 years (HR 10.19, 95%CI 1.28–80.93), therapy >14 days (7.71, 1.98–30.09) and total cumulative dose of daptomycin ≥10 g (5.30, 1.14–24.66). Conclusions: Clinicians should monitor cumulative daptomycin dosage to minimize DEP risk, and be cautious particularly in older patients when the total dose of daptomycin exceeds 10 g.
Occurrence and Survival of Livestock-Associated MRSA in Pig Manure and on Agriculture Fields
Authors: Astrup Lӕrke Boye , Hansen Julie Elvekjӕr , Pedersen Karl
Published: April 2021

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widespread in European pig production, and an increasing number of humans attract infections with this bacterium. Although most infections occur in humans with direct livestock contact, an increasing number of infections occur in humans without any established livestock contact. There have been speculations that at least some of these infections may be connected to the exposure of liquid pig manure for example spread as fertilizers. The present study therefore undertook to measure the presence of LA-MRSA in liquid pig manure and on the surface of soils fertilized with liquid manure and investigate the survival of the bacterium in manure. The results showed that LA-MRSA could be detected in 7 out of 20 liquid manure samples and in 12 out of 186 soil samples. However, the bacterium was not more frequently detected in samples collected after compared to before the spreading of liquid manure on the fields, thus suggesting that other sources of LA-MRSA on agriculture fields likely exist. The decimation time in liquid manure was >32 days at 5 °C in vitro but decreased with increasing temperature. Based on these results, liquid manure does not appear to be an important risk factor for human exposure to LA-MRSA.
Microbiological Profiles of Dental Implants in Metabolic Syndrome Patients: A Case-Control Study
Authors: Di Murro Bianca , Moretti Marta , De Smaele Enrico , Letizia Claudio , Lubrano Carla , Passarelli Pier Carmine , D’Addona Antonio , Pompa Giorgio , Papi Piero
Published: April 2021

There is a lack of knowledge on the possible influence of systemic conditions on peri-implantitis. The aim of this case-control study is to evaluate the difference in terms of oral pathogens’ concentrations in the peri-implant sulcus of a group of patients affected by metabolic syndrome (Mets) compared to healthy subjects. For each patient, peri-implant sulcular biofilm samples were obtained by inserting two sterile endodontic paper points in the deepest aspect of the peri-implant sulcus for 30 s. The quantitative real-time polymerase chain reaction was performed to evaluate total bacterial counts of six pathogens. Patients were screened for peri-implant diseases and clinical and radiographic parameters were recorded. A total of 50 patients was enrolled in the study, 25 affected by Mets and 25 healthy. Significantly higher bacterial counts were discovered for Aggregatibacter actinomycetemcomitans (p = 0.0008), Prevotella intermedia (p = 0.0477) and Staphylococcus aureus (p = 0.034) in MetS patients compared to healthy subjects. Performing a sub-group analysis, considering peri-implant status and dividing patients by MetS diagnosis, no statistically significant (p < 0.05) differences were found. For the first time, a correlation between MetS presence and a greater prevalence of some bacterial species in the peri-implant sulcus was reported, irrespectively from peri-implant status (health vs. disease).
Torque Requirements and the Influence of Pilot Holes on Orthodontic Miniscrew Microdamage
Authors: Jensen Sven W. , Jensen Emilija D. , Sampson Wayne , Dreyer Craig
Published: April 2021

This study aimed to quantify the microdamage to cortical bone of different thickness and the maximum insertion torque during orthodontic miniscrew implant (OMI) placement with and without a pilot hole. Forty-five porcine bone specimens were prepared with thicknesses of 1.5, 2 and 2.5 mm. Ten bone specimens per thickness had a pilot hole drilled prior to the insertion of an OMI, and the remaining 15 bone specimens had an OMI without a pilot hole inserted. Sequential staining was used to identify damage caused by bone preparation and surface microdamage from OMI insertion and confocal laser microscopy images were used to quantify damage characteristics. Of the five damage characteristics, only one decreased when a pilot hole was used for all bone specimens (p = 0.025), while two increased as cortical bone thickness increased (p = 0.0064, p = 0.0003). There was no evidence that maximum insertion torque differed according to pilot hole status (p = 0.1144) and increased as cortical bone thickness increased (p = 0.0001). The presence of a pilot hole had minimal effect on microdamage characteristics and no effect on maximum insertion torque. As cortical bone thickness increased, an increase in microdamage and in maximum insertion torque was observed.
Specific Modeling Issues on an Adaptive Winglet Skeleton
Authors: Ameduri Salvatore , Dimino Ignazio , Concilio Antonio , Mercurio Umberto , Pellone Lorenzo
Published: April 2021

Morphing aeronautical systems may be used for a number of aims, ranging from improving performance in specific flight conditions, to keeping the optimal efficiency over a certain parameters domain instead of confining it to a single point, extending the flight envelope, and so on. An almost trivial statement is that traditional skeleton architectures cannot be held as a structure modified from being rigid to deformable. That passage is not simple, as a structure that is able to be modified shall be designed and constructed to face those new requirements. What is not marginal, is that the new configurations can lead to some peculiar problems for both the morphing and the standard, supporting, elements. In their own nature, in fact, adaptive systems are designed to contain all the parts within the original geometry, without any “external adjoint”, such as nacelles or others. Stress and strain distribution may vary a lot with respect to usual structures and some particular modifications are required. Sometimes, it happens that the structural behavior does not match with the common experience and some specific adjustment shall be done to overcome the problem. What is reported in this paper is a study concerning the adaptation of the structural architecture, used to host a winglet morphing system, to make it accomplish the original requirements, i.e., allow the deformation values to be under the safety threshold. When facing that problem, an uncommon behavior of the finite element (FE) solver has been met: the safety factors appear to be tremendously dependent on the mesh size, so as to raise serious questions about the actual expected value, relevant for the most severe load conditions. On the other side, such singularities are more and more confined into single points (or single lines), as the mesh refines, so to evidence somehow the numerical effect behind those results. On the other side, standard engineering local methods to reduce the abovementioned strain peaks seem to work very well in re-distributing the stress and strain excesses to the whole system domain. The work does not intend to give an answer to the presented problem, being instead focused on describing its possible causes and its evident effects. Further work is necessary to detect the original source of such inconsistencies, and propose and test operative solutions. That will be the subject of the next steps of the ongoing research.
Inactivation of Listeria monocytogenes and Salmonella on Stainless Steel by a Piezoelectric Cold Atmospheric Plasma Generator
Authors: Gonzalez-Gonzalez Cid R. , Hindle Bethan J. , Saad Saliha , Stratakos Alexandros Ch.
Published: April 2021

Cold atmospheric pressure plasma (CAP) is a novel non-thermal technology that is gaining increasing importance as a decontamination method. Stainless steel is a widespread food contact surface used in food-processing environments. In this study, for the first time, a low-voltage piezoelectric CAP device that uses ambient air was assessed for its antimicrobial efficiency against Salmonella and Listeria monocytogenes. These inoculated on stainless steel at different exposure times (0–300 s), two different distances (10 and 20 mm), and two different cleanliness levels (clean and protein-soiled). Two inactivation models were compared to study the inactivation kinetics of the pathogens. The results showed that CAP treatment effectively reduced L. monocytogenes and Salmonella levels. The Weibull + tail model showed better goodness of fit than the Weibull model. Protein-soiled coupons showed a protective effect to cold plasma inactivation achieving lower reductions compared to clean stainless-steel coupons for both L. monocytogenes and Salmonella. Longer distances from the plasma source decreased the decontamination efficiency of CAP; however, the difference in pathogen reduction was less pronounced at longer exposure times. This study demonstrates the capacity of a low-voltage piezoelectric CAP device to effectively reduce the levels of both foodborne pathogens on stainless-steel surfaces and the potential to adopt this technology by the food industry as a disinfection process of surfaces to reduce cross-contamination and thus increase safety.
Risks and Benefits of Crew Reduction and/or Removal with Increased Automation on the Ship Operator: A Licensed Deck Officer’s Perspective
Authors: Hannaford Elspeth , Hassel Edwin Van
Published: April 2021

As autonomous technologies proliferate in the shipping industry, limited research has been conducted on its potential implications on the Licensed Deck Officer. This research examines the potential benefits and risks of increased onboard automation with the reduction and/or removal of onboard crew on the Licensed Deck Officer. Qualitative methods of literature review, survey, and individual semi-structured interviews were used. The rating scale method and Likert Bar Graph scaling approach convey survey results. A Theoretical Thematic Analysis was used to analyze interview data. Reliability, validity, and objectivity of Subject Matter Experts (SMEs) interviewed are part of the research strategy. It was found that many barriers exist to the implementation of autonomous vessels, mostly revolving around crew and vessel safety. Reducing crews and increasing shipboard automation have potentially negative effects on the Licensed Deck Officer, including sensor over-reliance, decreased situational awareness, and increased complacency, while providing no reduction in onboard duties or fatigue. Changes in navigational, manning, and liability regulation must be addressed by maritime authorities first. The shipping industry’s mindset is slow to change, but with this inevitable technology, the mariner will adapt. Insights generated from this research will benefit involved stakeholders to better understand and prepare for changes in the maritime industry due to the onset of autonomous shipping.
Feasibility of the Quantitative Assessment Method for CT Quality Control in Phantom Image Evaluation
Authors: Lee Ki Baek , Nam Ki Chang , Jang Ji Sung , Kim Ho Chul
Published: April 2021

Computed tomography (CT) quality control (QC) is regularly performed with standard phantoms, to bar faulty equipment from medical use. Its accuracy may be improved by replacing qualitative methods based on good visual distinction with pixel value-based quantitative methods. We hypothesized that statistical texture analysis (TA) that covers the entire phantom image would be a more appropriate tool. Therefore, our study devised a novel QC method based on the TA for contrast resolution (CR) and spatial resolution (SR) and proposed new, quantitative CT QC criteria. TA of CR and SR images on an American Association of Physicists in Medicine (AAPM) CT Performance Phantom were performed with nine CT scanner models. Six texture descriptors derived from first-order statistics of grayscale image histograms were analyzed. Principal component analysis was used to reveal descriptors with high utility. For CR evaluation, contrast and softness were the most accurate descriptors. For SR evaluation, contrast, softness, and skewness were the most useful descriptors. We propose the following ranges: contrast for CR, 29.5 ± 15%, for SR, 29 ± 10%; softness for CR, <0.015, for SR, <0.014; and skewness for SR, >−1.85. Our novel TA method may improve the assessment of CR and SR of AAPM phantom images.
Numerical Simulation of the Effect of Loading Angle on Initial Cracks Position Point: Application to the Brazilian Test
Authors: Bouali Meriem Fakhreddine , Bouassida Mounir
Published: April 2021

The Brazilian Test is the most used test to determine the indirect tensile strength for brittle materials like concrete. It has been observed that the success of the test depends on the cracks initiation point position and therefore the arch loading angle; a crack appears in the center of the disk when the test is valid. To this effect, using Fast Lagrangian of Continua code FLAC2D; numerical analyses were performed to study the impact of the arch loading angle on the initial crack’s position in a 70 mm diameter Brazilian disk of concrete and mortar under loading arch 2α which varies from 5–45°. The distribution of stresses and the tensile strength at the center of the Brazilian disk obtained numerically was closely similar to analytical and experimental existing solutions. The results showed that to obtain a meaningful and validated test with the most accurate indirect tensile strength, it is recommended to take a loading arch 2α ≥ 20° for the concrete and 2α ≥ 10° for the mortar.
Viscosity, Degree of Polymerization, Water Uptake, and Water Solubility Studies on Experimental Dichloro-BisGMA-Based Dental Composites
Authors: Al-Odayni Abdel-Basit , Saeed Waseem Sharaf , Khan Rawaiz , Al-Kahtani Abdullah , Aouak Taieb , Almutairi Khalid , Alrahlah Ali
Published: April 2021

The objective of this work was to investigate the advantages of using dichloro bisphenol A-glycidyl methacrylate (dCl-BisGMA) as a potential matrix for dental resin composites. A series of model composites containing 65 wt% resin (urethane dimethacrylate/triethylene glycol dimethacrylate/BisGMA as 1:3:1) and 35 wt% silanated silica were prepared. Thus, BisGMA was replaced by dCl-BisGMA as 0, 25, 50, and 100 wt% to obtain UTBC0, UTBC25, UTBC50, and UTBC100, respectively. The composites’ rheological properties, degree of double-bond conversion (DC), water sorption (WSP), and water solubility (WSL) were examined. The data revealed a statistically significant reduction in the complex viscosity of composites containing dCl-BisGMA, compared with UTBC0. No significant differences between DCs were detected (p < 0.05). A significant enhancement in the reduction of the dCl-BisGMA composite WSP was also detected, and conversely, WSL was increased. Although the viscosity, DC, and WSP characters were enhanced, a WSL increase is an undesirable development. However, WSL is supposedly caused by cyclization of small flexible chains, which is more likely to occur in the presence of hydrophobic monomers such as dCl-BisGMA and more prone to leaching than are crosslinked networks. We concluded that dCl-BisGMA is a monomer that could potentially be used as an alternative or in combination with traditional monomers, including BisGMA, in resin-based dental composites, and it deserves further investigation.
Fire Performance of Self-Tapping Screws in Tall Mass-Timber Buildings
Authors: Létourneau-Gagnon Mathieu , Dagenais Christian , Blanchet Pierre
Published: April 2021

Building elements are required to provide sufficient fire resistance based on requirements set forth in the National Building Code of Canada (NBCC). Annex B of the Canadian standard for wood engineering design (CSA O86-19) provides a design methodology to calculate the structural fire-resistance of large cross-section timber elements. However, it lacks at providing design provisions for connections. The objectives of this study are to understand the fire performance of modern mass timber fasteners such as self-tapping screws, namely to evaluate their thermo-mechanical behavior and to predict their structural fire-resistance for standard fire exposure up to two hours, as would be required for tall buildings in Canada. The results present the great fire performance of using self-tapping screws under a long time exposure on connections in mass timber construction. The smaller heated area of the exposed surface has limited thermal conduction along the fastener’s shanks and maintained their temperature profiles relatively low for two hours of exposure. Based on the heat-affected area, the study presents new design principles to determine the residual length of penetration that would provide adequate load-capacity of the fastener under fire conditions. It also allows determining safe fire-resistance values for unprotected fasteners in mass timber construction exposed up to two hours of standard fire exposure.
Biofilm Formation by Multidrug-Resistant Serotypes of Salmonella Isolated from Fresh Products: Effects of Nutritional and Environmental Conditions
Authors: Avila-Novoa María-Guadalupe , Guerrero-Medina Pedro-Javier , Navarrete-Sahagún Velia , Gómez-Olmos Itzel , Velázquez-Suárez Noemí-Yolanda , De la Cruz-Color Lucia , Gutiérrez-Lomelí Melesio
Published: April 2021

Salmonella serotypes can develop biofilms in fresh food products. This study focused on determining the antimicrobial resistance profile and the effects of different growth media and environmental conditions on biofilm formation by multidrug-resistant serotypes of Salmonella. All 49.4% of the Salmonella strains (five serotypes) were multidrug resistant. Assessment of the ability to form biofilms using the crystal violet staining method revealed that 95.6% of the strains of Salmonella were strong biofilm producers in 96-well polystyrene microtiter plates. Overall, 59.3% of the Salmonella strains showed the rdar (red dry and rough colony) morphotype, 2.1% pdar (pink dry and rough colony), 27.4% bdar (brown dry and rough colony) and 10.9% saw (smooth and white colony), at two temperatures (22 and 35 °C). Mono-species biofilms of Salmonella serotypes showed a mean cell density of 8.78 log10 CFU/cm2 ± 0.053 in TSBS (1/20 diluted TSB (tryptic soy broth) + 1% strawberry residues) and 8.43 log10 CFU/cm2 ± 0.050 in TSBA (1/20 diluted TSB + 1% avocado residues) on polypropylene type B (PP) (p < 0.05). In addition, epifluorescence microscopy and scanning electron microscopy (SEM) enabled visualizing the bacteria and extracellular polymeric substances of biofilms on PP. Salmonella form biofilms depending on the serotype of the strains and the environmental conditions. Mono-species biofilms formed by Salmonella serotypes respond to nutrient limitation with the use of simplified culture media such as TSBA and TSBS.
Hsp60 Quantification in Human Gastric Mucosa Shows Differences between Pathologies with Various Degrees of Proliferation and Malignancy Grade
Authors: Pitruzzella Alessandro , Burgio Stefano , Lo Presti Pietro , Ingrao Sabrina , Fucarino Alberto , Bucchieri Fabio , Cabibi Daniela , Cappello Francesco , Conway de Macario Everly , Macario Alberto J. L. , David Sabrina , Rappa Francesca
Published: April 2021

Background: Stomach diseases are an important sector of gastroenterology, including proliferative benign; premalignant; and malignant pathologies of the gastric mucosa, such as gastritis, hyperplastic polyps, metaplasia, dysplasia, and adenocarcinoma. There are data showing quantitative changes in chaperone system (CS) components in inflammatory pathologies and tumorigenesis, but their roles are poorly understood, and information pertaining to the stomach is scarce. Here, we report our findings on one CS component, the chaperone Hsp60, which we studied first considering its essential functions inside and outside mitochondria. Methods: We performed immunohistochemical experiments for Hsp60 in different samples of gastric mucosa. Results: The data obtained by quantitative analysis showed that the average percentages of Hsp60 were of 32.8 in normal mucosa; 33.5 in mild-to-moderate gastritis; 51.8 in severe gastritis; 58.5 in hyperplastic polyps; 67.0 in intestinal metaplasia; 89.4 in gastric dysplasia; and 92.5 in adenocarcinomas. Noteworthy were: (i) the difference between dysplasia and adenocarcinoma with the other pathologies; (ii) the progressive increase in Hsp60 from gastritis to hyperplastic polyp, gastric dysplasia, and gastric carcinoma; and (iii) the correlation of Hsp60 levels with histological patterns of cell proliferation and, especially, with tissue malignancy grades. Conclusions: This trend likely reflects the mounting need for cells for Hsp60 as they progress toward malignancy and is a useful indicator in differential diagnosis, as well as the call for research on the mechanisms underpinning the increase in Hsp60 and its possible roles in carcinogenesis.
Concurrent Engineering Model for the Implementation of New Products in the Textile Industry: A Case Study
Authors: Aguilar-Virgen Quetzalli , Castañeda-González Missael , Marquez-Benavides Liliana , Gonzalez-Vazquez Jose , Taboada-González Paul
Published: April 2021

Concurrent engineering (CE) aims to provide a collaborative environment in which all involved parties work simultaneously to optimize resources. However, some aspects can make it hard to implement in organizations. This study presents the development of a CE model and its implementation in a Mexican textile maquiladora. The information about the process was obtained using administrative and quality tools (brainstorming, Pareto and Ishikawa charts, flowcharts, spreadsheets). A CE model based on the management of new products was designed, in consideration of the enterprise culture. The workgroup evaluated the proposed model in two projects to verify the functionality. The time for the development and approval of prototypes was reduced from three months to one. Now the process has a robust design, having identified design problems, potential operator errors, and bottlenecks. Losses from raw material replacements and reworks decreased by 39.4%, and there was an additional cost saving of 22.86%. CE implementation is not easy; the firsts steps are to change the organizational culture and the training of employees to work collaboratively.
A Novel Magnetic Respiratory Sensor for Human Healthcare
Authors: Hwang Kee Young , Jimenez Valery Ortiz , Muchharla Baleeswaraiah , Eggers Tatiana , Le Anh-Tuan , Lam Vu Dinh , Phan Manh-Huong
Published: April 2021

Breathing is vital to life. Therefore, the real-time monitoring of a patient′s breathing pattern is crucial to respiratory rehabilitation therapies, such as magnetic resonance exams for respiratory-triggered imaging, chronic pulmonary disease treatment, and synchronized functional electrical stimulation. While numerous respiratory devices have been developed, they are often in direct contact with a patient, which can yield limited data. In this study, we developed a novel, non-invasive, and contactless magnetic sensing platform that can precisely monitor a patient′s breathing, movement, or sleep patterns, thus providing efficient monitoring at a clinic or home. A magneto-LC resonance (MLCR) sensor converts the magnetic oscillations generated by a patient′s breathing into an impedance spectrum, which allows for a deep analysis of one′s breath variation to identify respiratory-related diseases like COVID-19. Owing to its ultrahigh sensitivity, the MLCR sensor yields a distinct breathing pattern for each patient tested. It also provides an accurate measure of the strength of a patient′s breath at multiple stages as well as anomalous variations in respiratory rate and amplitude. The sensor can thus be applied to detect symptoms of COVID-19 in a patient, due to shortness of breath or difficulty breathing, as well as track the disease′s progress in real time.
Research on Prediction of Movable Fluid Percentage in Unconventional Reservoir Based on Deep Learning
Authors: Wang Jiuxin , Luo Yutian , Yang Zhengming , Zhao Xinli , Niu Zhongkun
Published: April 2021

In order to improve the measurement speed and prediction accuracy of unconventional reservoir parameters, the deep neural network (DNN) is used to predict movable fluid percentage of unconventional reservoirs. The Adam optimizer is used in the DNN model to ensure the stability and accuracy of the model in the gradient descent process, and the prediction effect is compared with the back propagation neural network (BPNN), K-nearest neighbor (KNN), and support vector regression model (SVR). During network training, L2 regularization is used to avoid over-fitting and improve the generalization ability of the model. Taking nuclear magnetic resonance (NMR) T2 spectrum data of laboratory unconventional core as input features, the influence of model hyperparameters on the prediction accuracy of reservoir movable fluids is also experimentally analyzed. Experimental results show that, compared with BPNN, KNN, and SVR, the deep neural network model has a better prediction effect on movable fluid percentage of unconventional reservoirs; when the model depth is five layers, the prediction accuracy of movable fluid percentage reaches the highest value, the predicted value of the DNN model is in high agreement with the laboratory measured value. Therefore, the movable fluid percentage prediction model of unconventional oil reservoirs based on the deep neural network model can provide certain guidance for the intelligent development of the laboratory’s reservoir parameter measurement.
Fatty Acid Profile, Lipid Quality and Squalene Content of Teff (Eragrostis teff (Zucc.) Trotter) and Amaranth (Amaranthus caudatus L.) Varieties from Ethiopia
Authors: Amare Endale , Grigoletto Luca , Corich Viviana , Giacomini Alessio , Lante Anna
Published: April 2021

Teff and amaranth are gluten-free cereals with significant nutritional and health benefits. However, they are underutilized and known in limited areas of the world. The present study evaluated the fatty acid profile, crude fat, squalene content and lipid quality of seven teff (Eragrostis teff (Zucc.) Trotter) and three amaranth (Amaranthus caudatus L.) varieties from Ethiopia. The fat content ranged from 2.92 to 3.34% (averaging 3.06%) and from 8.28 to 9.21% (averaging 8.6%) for teff and amaranth, respectively. Linoleic, oleic and palmitic acid were predominant in both teff and amaranth, accounting for approximately 89 and 85% of total fatty acid content, respectively. The saturated to unsaturated fatty acids ratio ranged from 0.30 to 0.32 in teff and from 0.38 to 0.40 in amaranth. The parameters used to describe lipid quality, i.e., thrombogenicity and atherogenicity indices, show that teff was superior over amaranth, suggesting a preference for the former for healthy food formulation. The squalene content of white amaranth (486.54 mg/100 g DM) was significantly higher than that of the other two varieties (327.54 and 340.81 mg/100 g DM for red and brown amaranth, respectively). In general, both gluten-free crops should be exploited for their potential as ingredients for the development of novel functional foods.
Nucleic Acids Chemistry and Engineering: Special Issue on Nucleic Acid Conjugates for Biotechnological Applications
Authors: Endoh Tamaki , Rozners Eriks , Ohtsuki Takashi
Published: April 2021

Nucleic acids not only store genetic information in their primary sequence but also exhibit biological functions through the formation of their unique structures [...]
Interactions between Cryptosporidium, Enterocytozoon, Giardia and Intestinal Microbiota in Bactrian Camels on Qinghai-Tibet Plateau, China
Authors: Wang Xiaoqi , Zhang Zhichao , Yin Weiwen , Zhang Qingxun , Wang Rujing , Duan Ziyuan
Published: April 2021

Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis are zoonotic pathogens commonly found in the intestinal tract of mammalian hosts including livestock and humans. The prevalence of these eukaryote microorganisms in domestic animals and their interaction with intestinal microbiota are not yet fully recognized. We analyzed the intestinal microbiota composition with metagenomics and functional characterization with Cluster of Orthologous (COG) in Bactrian camels, which were raised on Qinghai-Tibet Plateau, Northwest China. Thus, fecal samples were collected from the animals to determine the parasite infection and the profile of microbiota. Analysis of intestinal microbiota at genus level revealed important features of interaction between parasites infection and bacterial community. Coprococcus and Prevotella were more abundant while Akkermansia had lower relative abundance with E. bieneusi infection. Bacteria of Akkermansia, Lactococcus, Oxalobacter, Sphaerochaeta, Paludibacter, Fibrobacter, Anaerovibrio, Pseudomonas, Mogibacterium, Pseudoramibacter_Eubacterium, YRC22, Flexispira, SMB53, AF12, and Roseburia genera were found under-presented and Oscillospira genus over-presented when G. duodenalis infection was present. Meanwhile, Cryptosporidium spp. and E. bieneusi co-infected animals showed lower relative abundance of Allobaculum, Rikenella, Shuttleworthia, Epulopiscium, Bilophila, Dorea, Fibrobacter, and TG5. Results demonstrate important interaction between the intestinal parasites and microbiota, and provide informative link for understanding the co-evolution of zoonotic pathogens and bacteria in domestic animals.