bluuebunny/crossref_metadata_2025 huggingface dataset:
The Immune Memory Response of In Vitro-Polarised Th1, Th2, and Th17 Cells in the Face of Ovalbumin-Transgenic Leishmania major in a Mouse Model
Authors: Tedla Mebrahtu G. , Nahar Musammat F. , Every Alison L. , Scheerlinck Jean-Pierre Y.
Published: August 2024

Th1 and Th2 cytokines determine the outcome of Leishmania major infection and immune protection depends mainly on memory T cells induced during vaccination. This largely hinges on the nature and type of memory T cells produced. In this study, transgenic Leishmania major strains expressing membrane-associated ovalbumin (mOVA) and soluble ovalbumin (sOVA) were used as a model to study whether fully differentiated Th1/Th2 and Th17 cells can recall immune memory and tolerate pathogen manipulation. Naïve OT-II T cells were polarised in vitro into Th1/Th2 cells, and these cells were transferred adoptively into recipient mice. Following the transferral of the memory cells, the recipient mice were challenged with OVA transgenic Leishmania major and a wild-type parasite was used a control. The in vitro-polarised T helper cells continued to produce the same cytokine signatures after being challenged by both forms of OVA-expressing Leishmania major parasites in vivo. This suggests that antigen-experienced cells remain the same or unaltered in the face of OVA-transgenic Leishmania major. Such ability of these antigen-experienced cells to remain resilient to manipulation by the parasite signifies that vaccines might be able to produce immune memory responses and defend against parasitic immune manipulation in order to protect the host from infection.
The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells
Authors: Ma Jun , Huang Ruixue , Zhang Huai , Liu Dongju , Dong Xiaodong , Xiong Yan , Xiong Xianrong , Lan Daoliang , Fu Wei , He Honghong , Li Jian , Yin Shi
Published: August 2024

Fumonisin B1 (FB1), a mycotoxin produced by Fusarium species, is prevalent in crops and animal feed, posing significant health risks to livestock and humans. FB1 induces oxidative stress in Sertoli cells, destroys testicular structure, and affects spermatogenesis. However, methods to mitigate the reproductive toxicity of FB1 in testes remain unknown. Quercetin, a natural flavonoid antioxidant, may offer protective benefits. This study investigated the protective effects and mechanisms of quercetin against FB1-induced reproductive toxicity in TM4 cells (a Sertoli cell line). The results indicated that 40 μM quercetin improved cell viability, reduced apoptosis, and preserved cell functions. Quercetin also decreased reactive oxygen species (ROS) levels in TM4 cells exposed to FB1, enhanced the expression of antioxidant genes, and improved mitochondrial membrane potential. Compared with FB1 alone, the combination of quercetin and FB1 increased ATP levels, as well as pyruvate and lactic acid, the key glycolysis products. Furthermore, this combination elevated the mRNA and protein expression of glycolysis-related genes, including glucose-6-phosphate isomerase 1 (Gpi1), hexokinase 2 (Hk2), aldolase (Aldoa), pyruvate kinase, muscle (Pkm), lactate dehydrogenase A (Ldha) and phosphofructokinase, liver, B-type (Pfkl). Quercetin also boosted the activity of PKM and LDHA, two crucial glycolytic enzymes. In summary, quercetin mitigates FB1-induced toxicity in TM4 cells by reducing ROS levels and enhancing glycolysis. This study offers new insights into preventing and treating FB1-induced toxic damage to the male reproductive system and highlights the potential application of quercetin.
The Mitogenome of the Haecon-5 Strain of Haemonchus contortus and a Comparative Analysis of Its Nucleotide Variation with Other Laboratory Strains
Authors: Zheng Yuanting , Young Neil D. , Song Jiangning , Gasser Robin B.
Published: August 2024

Haemonchus contortus (the barber’s pole worm)—a highly pathogenic gastric nematode of ruminants—causes significant economic losses in the livestock industry worldwide. H. contortus has become a valuable model organism for both fundamental and applied research (e.g., drug and vaccine discovery) because of the availability of well-defined laboratory strains (e.g., MHco3(ISE).N1 in the UK and Haecon-5 in Australia) and genomic, transcriptomic and proteomic data sets. Many recent investigations have relied heavily on the use of the chromosome-contiguous genome of MHco3(ISE).N1 in the absence of a genome for Haecon-5. However, there has been no genetic comparison of these and other strains to date. Here, we assembled and characterised the mitochondrial genome (14.1 kb) of Haecon-5 and compared it with that of MHco3(ISE).N1 and two other strains (i.e., McMaster and NZ_Hco_NP) from Australasia. We detected 276 synonymous and 25 non-synonymous single nucleotide polymorphisms (SNPs) within Haecon-5. Between the Haecon-5 and MHco3(ISE).N1 strains, we recorded 345 SNPs, 31 of which were non-synonymous and linked to fixed amino acid differences in seven protein-coding genes (nad5, nad6, nad1, atp6, nad2, cytb and nad4) between these strains. Pronounced variation (344 and 435 SNPs) was seen between Haecon-5 and each of the other two strains from Australasia. The question remains as to what impact these mitogenomic mutations might have on the biology and physiology of H. contortus, which warrants exploration. The high degree of mitogenomic variability recorded here among these strains suggests that further work should be undertaken to assess the nature and extent of the nuclear genomic variation within H. contortus.
LC/MS-Based Untargeted Lipidomics Reveals Lipid Signatures of Sarcopenia
Authors: Yang Qianwen , Zhang Zhiwei , He Panpan , Mao Xueqian , Jing Xueyi , Hu Ying , Jing Lipeng
Published: August 2024

Sarcopenia, a multifactorial systemic disorder, has attracted extensive attention, yet its pathogenesis is not fully understood, partly due to limited research on the relationship between lipid metabolism abnormalities and sarcopenia. Lipidomics offers the possibility to explore this relationship. Our research utilized LC/MS-based nontargeted lipidomics to investigate the lipid profile changes as-sociated with sarcopenia, aiming to enhance understanding of its underlying mechanisms. The study included 40 sarcopenia patients and 40 control subjects matched 1:1 by sex and age. Plasma lipids were detected and quantified, with differential lipids identified through univariate and mul-tivariate statistical analyses. A weighted correlation network analysis (WGCNA) and MetaboAna-lyst were used to identify lipid modules related to the clinical traits of sarcopenia patients and to conduct pathway analysis, respectively. A total of 34 lipid subclasses and 1446 lipid molecules were detected. Orthogonal partial least squares discriminant analysis (OPLS-DA) identified 80 differen-tial lipid molecules, including 38 phospholipids. Network analysis revealed that the brown module (encompassing phosphatidylglycerol (PG) lipids) and the yellow module (containing phosphati-dylcholine (PC), phosphatidylserine (PS), and sphingomyelin (SM) lipids) were closely associated with the clinical traits such as maximum grip strength and skeletal muscle mass (SMI). Pathway analysis highlighted the potential role of the glycerophospholipid metabolic pathway in lipid me-tabolism within the context of sarcopenia. These findings suggest a correlation between sarcopenia and lipid metabolism disturbances, providing valuable insights into the disease’s underlying mechanisms and indicating potential avenues for further investigation.
Ubiquitination Insight from Spinal Muscular Atrophy—From Pathogenesis to Therapy: A Muscle Perspective
Authors: Bolado-Carrancio Alfonso , Tapia Olga , Rodríguez-Rey José C.
Published: August 2024

Spinal muscular atrophy (SMA) is one of the most frequent causes of death in childhood. The disease’s molecular basis is deletion or mutations in the SMN1 gene, which produces reduced survival motor neuron protein (SMN) levels. As a result, there is spinal motor neuron degeneration and a large increase in muscle atrophy, in which the ubiquitin–proteasome system (UPS) plays a significant role. In humans, a paralogue of SMN1, SMN2 encodes the truncated protein SMNΔ7. Structural differences between SMN and SMNΔ7 affect the interaction of the proteins with UPS and decrease the stability of the truncated protein. SMN loss affects the general ubiquitination process by lowering the levels of UBA1, one of the main enzymes in the ubiquitination process. We discuss how SMN loss affects both SMN stability and the general ubiquitination process, and how the proteins involved in ubiquitination could be used as future targets for SMA treatment.
Lipoprotein(a) in Children and Adolescents: Risk or Causal Factor for Cardiovascular Disease? A Narrative Review
Authors: Capra Maria Elena , Biasucci Giacomo , Banderali Giuseppe , Pederiva Cristina
Published: August 2024

The evaluation of serum Lp(a) values in childhood and adolescence has been widely debated, and in the last few years, many authors have tried to better define Lp(a) role in atherosclerosis pathogenesis, starting from childhood. In our narrative review, we have evaluated the main historical stages of Lp(a) studies in childhood, trying to focus on pathogenic mechanisms linked to elevated serum Lp(a) values, starting from ischemic stroke and vascular damage, and to its possible direct involvement in premature atherosclerosis from childhood onwards. Historic manuscripts on Lp(a) in pediatric patients have mainly focused on serum Lp(a) values and increased stroke risk. More recently, many studies have evaluated Lp(a) as a coronary vascular disease (CVD) risk factor starting from childhood, especially related to a positive family history of premature CVD. Finally, only a few studies evaluated the role of Lp(a) in premature atherosclerotic processes and endothelial and vascular damage in pediatric patients. Lastly, we have hypothesized a future perspective, with the hope that plasma Lp(a) levels will be treated with a tailored pharmacologic approach, and Lp(a) will become a precocious therapeutic target to control the atherosclerotic pathways from the first years of life.
Emergence and Comparative Genome Analysis of Salmonella Ohio Strains from Brown Rats, Poultry, and Swine in Hungary
Authors: Szmolka Ama , Lancz Zsuzsanna Sréterné , Rapcsák Fanni , Egyed László
Published: August 2024

Rats are particularly important from an epidemiological point of view, because they are regarded as reservoirs for diverse zoonotic pathogens including enteric bacteria. This study is the first to report the emergence of Salmonella serovar Ohio in brown rats (Rattus norvegicus) and food-producing animals in Hungary. We first reveal the genomic diversity of the strains and their phylogenomic relationships in the context of the international collection of S. Ohio genomes. This pathogen was detected in 4.3% (4/92) of rats, captured from multiple sites in Hungary. A whole-genome-based genotype comparison of S. Ohio, Infantis, Enteritidis, and Typhimurium strains showed that 76.4% (117/153) of the virulence and antimicrobial resistance genes were conserved among these serovars, and none of the genes were specific to S. Ohio. All S. Ohio strains lacked virulence and resistance plasmids. The cgMLST phylogenomic comparison highlighted a close genetic relationship between rat and poultry strains of S. Ohio from Hungary. These strains clustered together with the international S. Ohio genomes from aquatic environments. Overall, this study contributes to our understanding of the epidemiology of Salmonella spp. in brown rats and highlights the importance of monitoring to minimize the public health risk of rodent populations. However, further research is needed to understand the route of infection and evolution of this serovar.
Genome Mining and Genetic Manipulation Reveal New Isofuranonaphthoquinones in Nocardia Species
Authors: Poudel Purna Bahadur , Dhakal Dipesh , Magar Rubin Thapa , Parajuli Niranjan , Sohng Jae Kyung
Published: August 2024

The identification of specialized metabolites isolated from microorganisms is urgently needed to determine their roles in treating cancer and controlling multidrug-resistant pathogens. Naphthoquinones act as anticancer agents in various types of cancers, but some toxicity indicators have been limited in their appropriate application. In this context, new isofuranonaphthoquinones (ifnq) that are less toxic to humans could be promising lead compounds for developing anticancer drugs. The aim of this study is to identify and characterize novel furanonaphthoquinones (fnqs) from Nocardia sp. CS682 and to evaluate their potential therapeutic applications. Analysis of the genome of Nocardia sp. CS682 revealed the presence of a furanonaphthoquinone (fnq) gene cluster, which displays a similar genetic organization and high nucleotide sequence identity to the ifnq gene cluster from Streptomyces sp. RI-77, a producer of the naphthoquinones JBIR-76 and JBIR-77. In this study, the overexpression of the Streptomyces antibiotic regulatory protein (SARP) in Nocardia sp. CS682DR (nargenicin gene-deleted mutant) explicitly produced new fnqs, namely, NOC-IBR1 and NOC-IBR2. Subsequently, the role of the SARP regulator was confirmed by gene inactivation using CRISPR-Cas9 and complementation studies. Furthermore, antioxidant, antimicrobial, and cytotoxicity assays were performed for the isolated compounds, and it was found that NOC-IBR2 exhibited superior activities to NOC-IBR1. In addition, a flexible methyltransferase substrate, ThnM3, was found to be involved in terminal methylation of NOC-IBR1, which was confirmed by in vitro enzyme assays. Thus, this study supports the importance of genome mining and genome editing approaches for exploring new specialized metabolites in a rare actinomycete called Nocardia.
Role of Extracellular Vesicles in Crohn’s Patients on Adalimumab Who Received COVID-19 Vaccination
Authors: De Luca Maria , Musio Biagia , Balestra Francesco , Arrè Valentina , Negro Roberto , Depalo Nicoletta , Rizzi Federica , Mastrogiacomo Rita , Panzetta Giorgia , Donghia Rossella , Pesole Pasqua Letizia , Coletta Sergio , Piccinno Emanuele , Scalavino Viviana , Serino Grazia , Maqoud Fatima , Russo Francesco , Orlando Antonella , Todisco Stefano , Mastrorilli Pietro , Curri Maria Lucia , Gallo Vito , Giannelli Gianluigi , Scavo Maria Principia
Published: August 2024

Crohn’s disease (CD) is a type of inflammatory bowel disease (IBD) affecting the gastrointestinal tract that can also cause extra-intestinal complications. Following exposure to the mRNA vaccine BNT162b2 (Pfizer-BioNTech) encoding the SARS-CoV-2 Spike (S) protein, some patients experienced a lack of response to the biological drug Adalimumab and a recrudescence of the disease. In CD patients in progression, resistant to considered biological therapy, an abnormal increase in intestinal permeability was observed, more often with a modulated expression of different proteins such as Aquaporin 8 (AQP8) and in tight junctions (e.g., ZO-1, Claudin1, Claudin2, Occludin), especially during disease flares. The aim of this study is to investigate how the SARS-CoV-2 vaccine could interfere with IBD therapy and contribute to disease exacerbation. We investigated the role of the SARS-CoV-2 Spike protein, transported by extracellular vesicles (EVs), and the impact of various EVs components, namely, exosomes (EXOs) and microvesicles (MVs), in modulating the expression of molecules involved in the exacerbation of CD, which remains unknown.
Human Primary Lens Epithelial Cultures on Basal Laminas Studied by Synchrotron-Based FTIR Microspectroscopy for Understanding Posterior Capsular Opacification
Authors: Andjelic Sofija , Hawlina Marko
Published: August 2024

Human primary lens epithelial cultures serve as an in vitro model for posterior capsular opacification (PCO) formation. PCO occurs when residual lens epithelial cells (LECs) migrate and proliferate after cataract surgery, differentiating into fibroblastic and lens fiber-like cells. This study aims to show and compare the bio-macromolecular profiles of primary LEC cultures and postoperative lens epithelia LECs on basal laminas (bls), while also analyzing bls and cultured LECs separately. Using synchrotron radiation-based Fourier transform infrared (SR-FTIR) (Bruker, Karlsruhe, Germany) microspectroscopy at the Spanish synchrotron light source ALBA, we observed that the SR-FTIR measurements were predominantly influenced by the strong collagen absorbance of the bls. Cultured LECs on bls showed a higher collagen contribution, indicated by higher vas CH3, CH2 and CH3 wagging and deformation, and the C–N stretching of collagen. In contrast, postoperative LECs on bls showed a higher cell contribution, indicated by the vsym CH2 peak and the ratio between vas CH2 and vas CH3 peaks. The primary difference revealed using SR-FTIR is the greater LEC contribution in spectra recorded from postoperative lens epithelia compared to cultured LECs on bls. IR spectra for bl, cultured LECs and postoperative lens epithelia could be valuable for future research.
Emerging Therapeutic Approaches and Genetic Insights in Stargardt Disease: A Comprehensive Review
Authors: Ghenciu Laura Andreea , Hațegan Ovidiu Alin , Stoicescu Emil Robert , Iacob Roxana , Șișu Alina Maria
Published: August 2024

Stargardt disease, one of the most common forms of inherited retinal diseases, affects individuals worldwide. The primary cause is mutations in the ABCA4 gene, leading to the accumulation of toxic byproducts in the retinal pigment epithelium (RPE) and subsequent photoreceptor cell degeneration. Over the past few years, research on Stargardt disease has advanced significantly, focusing on clinical and molecular genetics. Recent studies have explored various innovative therapeutic approaches, including gene therapy, stem cell therapy, and pharmacological interventions. Gene therapy has shown promise, particularly with adeno-associated viral (AAV) vectors capable of delivering the ABCA4 gene to retinal cells. However, challenges remain due to the gene’s large size. Stem cell therapy aims to replace degenerated RPE and photoreceptor cells, with several clinical trials demonstrating safety and preliminary efficacy. Pharmacological approaches focus on reducing toxic byproduct accumulation and modulating the visual cycle. Precision medicine, targeting specific genetic mutations and pathways, is becoming increasingly important. Novel techniques such as clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 offer potential for directly correcting genetic defects. This review aims to synthesize recent advancements in understanding and treating Stargardt disease. By highlighting breakthroughs in genetic therapies, stem cell treatments, and novel pharmacological strategies, it provides a comprehensive overview of emerging therapeutic options.
Earlier Decision on Detection of Ransomware Identification: A Comprehensive Systematic Literature Review
Authors: Albshaier Latifa , Almarri Seetah , Rahman M. M. Hafizur
Published: August 2024

Cybersecurity is normally defined as protecting systems against all kinds of cyberattacks; however, due to the rapid and permanent expansion of technology and digital transformation, the threats are also increasing. One of those new threats is ransomware, which is a form of malware that aims to steal user’s money. Ransomware is a form of malware that encrypts a victim’s files. The attacker then demands a ransom from the victim to restore access to the data upon a large payment. Ransomware is a way of stealing money in which a user’s files are encrypted and the decrypted key is held by the attacker until a ransom amount is paid by the victim. This systematic literature review (SLR) highlights recent papers published between 2020 and 2024. This paper examines existing research on early ransomware detection methods, focusing on the signs, frameworks, and techniques used to identify and detect ransomware before it causes harm. By analyzing a wide range of academic papers, industry reports, and case studies, this review categorizes and assesses the effectiveness of different detection methods, including those based on signatures, behavior patterns, and machine learning (ML). It also looks at new trends and innovative strategies in ransomware detection, offering a classification of detection techniques and pointing out the gaps in current research. The findings provide useful insights for cybersecurity professionals and researchers, helping guide future efforts to develop strong and proactive ransomware detection systems. This review emphasizes the need for ongoing improvements in detection technologies to keep up with the constantly changing ransomware threat landscape.
Enhanced Risk of Gastroesophageal Reflux Disease and Esophageal Complications in the Ulcerative Colitis Population
Authors: Wang Xiaoliang , Almetwali Omar , Wang Jiayan , Wright Zachary , Patton-Tackett Eva D. , Roy Stephen , Tu Lei , Song Gengqing
Published: August 2024

Background: Although heartburn and reflux are frequently reported in ulcerative colitis [UC], the correlation between UC and gastroesophageal reflux disease [GERD], and its complications, esophageal stricture and Barrett’s esophagus [BE], is not well understood. This study aims to examine the prevalence and associated risk of GERD and its complications within the UC population. Methods: We analyzed the National Inpatient Sample (NIS) dataset, consisting of 7,159,694 patients, comparing GERD patients with and without UC to those without GERD. We assessed the degree of colonic involvement in UC and the occurrence of esophageal complications. Bivariate analyses were conducted using the chi-squared test or Fisher exact test (two-tailed). Results: A higher prevalence of GERD (23.0% vs. 16.5%) and GERD phenotypes, such as non-erosive reflux disease (NERD) (22.3% vs. 16%) and erosive esophagitis (EE) (1.2% vs. 0.6%), was found in UC patients (p < 0.01), including pancolitis, proctitis, proctosigmoiditis, left-sided colitis, and indetermined UC (with undefined colonic involvement). UC patients were more likely to develop GERD (1.421), NERD (1.407), and EE (1.681) (p < 0.01). A higher prevalence of esophageal stricture (16.9 vs. 11.4 per 10,000 patients) and BE without dysplasia (94.5 vs. 39.3 per 10,000 patients) was found in UC (p < 0.05). The odds of developing BE without dysplasia were higher (1.892) in patients with UC (p < 0.01), including ulcerative pancolitis, proctitis, and indeterminate UC (OR of 1.657, 3.328, and 1.996, respectively) (p < 0.05). Conclusions: Our study demonstrates an increased risk of developing GERD and its complications in UC. This highlights the importance of vigilant monitoring and early intervention to minimize associated GERD-related risks in patients with UC.
Association of Lifestyle Behaviors with Quality of Life in Patients with COPD: A Cross-Sectional Study in Primary Care
Authors: Bouloukaki Izolde , Christodoulakis Antonios , Margetaki Katerina , Tsiligianni Ioanna
Published: August 2024

Background/Objectives: The association between healthy lifestyle behaviors and their effect on quality of life among patients with COPD remains unclear. Therefore, the aim of this study was to explore the lifestyle behaviors and their association with the disease-specific quality of life among a primary care population with COPD in Greece. Methods: This cross-sectional study included 236 participants aged 40 years and older from the COCARE COPD study. The healthy lifestyle index (HLI) was created based on smoking, alcohol consumption, BMI, physical activity, and sleep duration, with each factor categorized as either healthy (1) or unhealthy (0). The HLI ranged from 0 (least healthy) to 5 (healthiest). COPD-specific quality of life was assessed using the COPD assessment test (CAT), where higher scores indicate poorer health status. Multiple logistic regression was used to analyze the association between HLI and CAT scores, adjusting for confounders. Results: Half of the participants were non-smokers or former smokers, while 92% reported consuming low levels of alcohol (less than 14 units per week). Additionally, 56% had a BMI below 30, indicating they were not obese. Surprisingly, only 32% engaged in regular exercise, with at least 150 min per week, and only 25% reported getting adequate sleep, ranging from 7 to 9 h per night. Importantly, poorer health status was inversely associated with non/former smoking (OR: 0.543, 95% CI: 0.282–1.049), physical activity (OR: 0.238, 95% CI: 0.122–0.463), and adequate sleep (OR: 0.337, 95% CI: 0.160–0.710). Patients with higher HLI scores were less likely to have poor health status. Conclusions: In conclusion, our findings indicate that a significant proportion of patients with COPD failed to adhere to a minimum of three out of five healthy behaviors. Additionally, a higher number of healthy lifestyle factors defined by a high HLI score were independently associated with a better disease-specific quality of life. This is particularly important for COPD where quality of life is in the epicenter of management. Therefore, healthcare providers could significantly improve the management of COPD and patient outcomes by targeting and improving these lifestyle behaviors with targeted and holistic intervention strategies.
Multifunctional Iron Oxide Nanoparticles as Promising Magnetic Biomaterials in Drug Delivery: A Review
Authors: Vasić Katja , Knez Željko , Leitgeb Maja
Published: August 2024

A wide range of applications using functionalized magnetic nanoparticles (MNPs) in biomedical applications, such as in biomedicine as well as in biotechnology, have been extensively expanding over the last years. Their potential is tremendous in delivery and targeting systems due to their advantages in biosubstance binding. By applying magnetic materials-based biomaterials to different organic polymers, highly advanced multifunctional bio-composites with high specificity, efficiency, and optimal bioavailability are designed and implemented in various bio-applications. In modern drug delivery, the importance of a successful therapy depends on the proper targeting of loaded bioactive components to specific sites in the body. MNPs are nanocarrier-based systems that are magnetically guided to specific regions using an external magnetic field. Therefore, MNPs are an excellent tool for different biomedical applications, in the form of imaging agents, sensors, drug delivery targets/vehicles, and diagnostic tools in managing disease therapy. A great contribution was made to improve engineering skills in surgical diagnosis, therapy, and treatment, while the advantages and applicability of MNPs have opened up a large scope of studies. This review highlights MNPs and their synthesis strategies, followed by surface functionalization techniques, which makes them promising magnetic biomaterials in biomedicine, with special emphasis on drug delivery. Mechanism of the delivery system with key factors affecting the drug delivery efficiency using MNPs are discussed, considering their toxicity and limitations as well.
Experimental Investigation on the Impact of Sand Particle Size on the Jet Pump Wall Surface Erosion
Authors: Qian Heng , Liu Jian , Xu Maosen , Fan Chuanhao , Duan Zhenhua
Published: August 2024

Silt removal is crucial for maintaining navigable waterways in harbors. Jet pumps, without moving parts, are highly suitable for underwater operations such as channel dredging in port environments. Despite their structural advantages in slurry handling, the prolonged transport of solid–liquid two-phase flows can lead to wear on the wall materials, resulting in decreased efficiency and potential pump failure. The wear characteristics of the jet pump walls due to sand particles of varying grain sizes were experimentally investigated. The characteristic of the sands having a higher distribution above the axis as they enter the jet pump was captured by a high-speed camera. The experiment recorded the variations in mass loss at different sections of the jet pump over a period of 120 h, identifying that backflow within the throat region is a significant contributor to wall wear. Scanning electron microscopy was employed to examine the microstructure of the abraded pump surfaces. It was found that there are noticeable differences in the surface wear microstructure across various pump areas, and that particles of different grain sizes result in distinct wear patterns on the pump surfaces. The underlying causes of this phenomenon were discussed from the perspective of particle motion.
Study on the Effect of Thermal Characteristics of Grease-Lubricated High-Speed Silicon Nitride Full Ceramic Ball Bearings in Motorized Spindles
Authors: Wang Yonghua , Li Songhua , Wei Chao , Liu Bo , Zhang Yu , Lin Gefei , Wang Kun , Zhao Jining
Published: August 2024

Grease lubrication is cost-effective and low-maintenance for motorized spindles, but standard steel bearings can fail at high speeds. This study focuses on high-speed full ceramic ball bearings lubricated with grease. The coefficient of friction torque in the empirical formula is corrected by establishing the heat generation model of full ceramic ball bearing and combining it with experiments. A simulation model of grease flow is established to study the influence of grease filling amount on grease distribution. The simulation model of the temperature field of a full ceramic ball bearing is established to analyze the influence of rotating speed on bearing heat generation, and experiments verify the calculation results of the theoretical model. The results show that an optimal grease filling amount of 15~25% ensures even distribution without accumulation. Additionally, when the amount of grease is constant, the outer ring temperature increases with higher rotating speeds. The test results show that when the grease filling is 0.9~1.2 g, it accounts for about 9~12% of the volume of the bearing cavity, and the temperature of the outer ring is the lowest. At a rotation speed of 24,000 rpm, the outer ring temperature of the grease-lubricated bearing is 50.1 °C, indicating a reasonable range for use in motorized spindles. It provides a theoretical basis for the optimization design of macro-structural parameters of full ceramic ball bearings in the future, which can minimize heat generation and maximize bearing capacity.
Influence of Lubrication Cycle Parameters on Hydrodynamic Linear Guides through Simultaneous Monitoring of Oil Film Pressure and Floating Heights
Authors: Ibrar Burhan , Wittstock Volker , Regel Joachim , Dix Martin
Published: August 2024

Hydrodynamic linear guides in machine tools offer a high load capacity and excellent damping characteristics, improving stability, precision, and vibration reduction. This study builds on previous research where floating heights were verified with a simulation model limited to measured floating heights. Advancements include incorporating pressure sensors into a fixed steel rail, enabling simultaneous measurement of oil film pressure and floating heights for a comprehensive understanding of lubrication conditions within the lubrication gap. The experimental results explore the effects of different lubrication methods, providing valuable insights into cavitation and lubrication adequacy. The results demonstrate the feasibility of utilizing pressure sensors to measure oil film pressure within the lubrication gap, providing a nuanced understanding of lubrication dynamics. By measuring both floating heights and pressure measurement, distinctions between hydrodynamic lubrication, mixed friction regions, and instances of lubricant deficiency become readily discernible. The variations in real-time oil film pressure and floating heights help to optimize the lubrication cycle for hydrodynamic linear guides, enhancing system performance and longevity.
Electrochemical Corrosion Behavior and the Related Mechanism of Ti3SiC2/Cu Composites in a Strong Acid Environment
Authors: Zhang Rui , Du Chengzhi , Liu Fuyan , Wu Chenlong
Published: August 2024

The electrochemical corrosion behaviors of Ti3SiC2/Cu composites in harsh media including dilute HNO3 and concentrated H2SO4 were studied in detail and the related corrosion mechanisms were explored. Under open-circuit potential, the corrosion resistance of Ti3SiC2/Cu in dilute HNO3 was worse than that in concentrated H2SO4. In dilute HNO3, Ti3SiC2/Cu exhibited a typical passivation character with a narrow passivation interval. During the corrosion process, the dissolution of Cu-Si compounds resulted in the destruction of the passivation layer on the surface. Additionally, with the increasing of the potentials, the oxidation of Cu and Si atoms led to the generation of the oxide film again on the surface. In concentrated H2SO4, the Ti3SiC2/Cu composite was covered by a double-layered passivation layer, which was composed of an internal layer of TiO2 and an external layer of Cu2O and SiO2. This was because Cu diffused into the surface and was oxidized into Cu2O, which formed a denser oxidized film with SiO2. In addition, it was found that Ti3SiC2/Cu has better corrosion resistance in concentrated H2SO4.
Mechanical Properties of Ultra-High-Performance Concrete with Amorphous Alloy Fiber: Surface Modification by Silane Coupling Agent KH-550
Authors: Wang Dawei , Liu Runqing , Wang Song , Ma Xin
Published: August 2024

Amorphous alloy fiber has the advantages of high tensile strength and high corrosion resistance compared with steel fiber, but its interfacial bonding with cement matrix is poor and requires surface modification treatment. In this study, the surface modification of amorphous alloy fiber was carried out by using silane coupling agent KH-550 solution, and its effect on the mechanical properties of ultra-high-performance concrete was investigated. The results showed that the amorphous alloy fibers modified with 15% concentration silane coupling agent KH-550 solution can effectively improve the mechanical properties of the ultra-high-performance concrete, where the interfacial bond strength with the cement matrix reached 3.29 MPa and the roughness reached 3.85. The compressive strength, flexural strength, tensile strength, and peak stress of the ultra-high-performance concrete mixed with modified amorphous alloy fibers could reach up to 133.6 MPa, 25.5 MPa, 8.32 MPa, and 114.26 MPa, respectively, which were 2.9%, 6.3%, 10.9%, and 4.3% higher than those of the ultra-high-performance concrete with unmodified amorphous alloy fibers. As the surface of the fiber was modified, its properties changed and the bonding effect with the cement matrix was better, which in turn improved the mechanical properties of the ultra-high-performance concrete.
Impact Testing in Implant-Supported Prostheses and Natural Teeth: A Systematic Review of Properties and Performance
Authors: Martí-Vigil Jordi , Casamitjana Joan , Marimon Xavier , Cerrolaza Miguel , Medina-Gálvez Raul , Cantó-Navés Oriol , Ferrer Miquel , Cabratosa-Termes Josep
Published: August 2024

Dental implants offer an effective solution for partial and total edentulism, but mechanical and biological complications exist. Furthermore, high occlusal loads challenge implants and lead to potential failures. This review focuses on impact testing in contrast to incremental and static tests, an underexplored aspect of assessing daily loads on implants, bringing to light potential complications. The review examines studies employing impact forces to assess implant-supported prostheses and natural teeth properties, highlighting their significance in dental research. A systematic search following PRISMA guidelines identified 21 relevant articles out of 224, emphasizing studies employing impact forces to evaluate various aspects of dental implant treatments. The diverse applications of impact forces in dental research were categorized into tooth structure, restorative materials, interface evaluation, implant properties, and finite element models. Some studies showed the significance of impact forces in assessing stress distribution, shock absorption, and biomechanical response. Impact testing is a critical tool for understanding the daily forces on implants. Despite diverse experimental approaches, a lack of standardized protocols complicates the systematization of the results and, therefore, the conclusions. This review highlights the need for consistent methodologies in impact testing studies for future research on implant-supported prostheses.
Novel Workstation Module and Method for Automatic Blanking of Surgical Forceps
Authors: Du Xianzhen , Li Jiapeng , Wang Haochen , Li Zhenyu , Li Yusheng , Li Zhiyuan
Published: August 2024

During the manufacturing of surgical forceps, the flashes of the blanks need to be removed. Manual production has problems such as high labor intensity, low efficiency, and high-risk factors. To solve this problem and realize fully automatic resection, a novel modular workstation was designed and a corresponding process method was proposed. The workstation adopts robots, non-standard automation equipment, and image recognition technology instead of manual loading and blanking, but the blank storage still needs to be performed manually. The critical components were selected according to the workstation design scheme and process method, and the control system design was completed. The reliability of the separation unit was studied through a test platform, and the failure problem caused by uneven force was solved using a blank locking device, which showed that the separation success rate was stabilized at 100%. The detection speed of the image recognition system can reach 100 ms/piece, and the product qualification rate can reach 95.7%. The advantages of the workstation in terms of output and productivity were further analyzed by comparing it to manual production, where the average daily output increased by 12.5% (4500 pieces). In addition, the results of long-term test experiments and power consumption comparison tests showed that the workstations are highly stable and consume little additional power.
Optimization of Hot Embossing Condition Using Taguchi Method and Evaluation of Microchannels for Flexible On-Chip Proton-Exchange Membrane Fuel Cell
Authors: Huang Yubo , Gao Han , Wu Zhiheng , Xiao Hongyang , Xia Cao , Xia Yuanlin , Wang Zhuqing
Published: August 2024

Hot embossing is a manufacturing technique used to create microchannels on polymer substrates. In recent years, microchannel fabrication technology based on hot embossing has attracted considerable attention due to its convenience and low cost. A new evaluation method of microchannels, as well as an approach to obtaining optimal hot embossing conditions based on the Taguchi method, is proposed in this paper to fabricate precise microchannels for a flexible proton-exchange membrane fuel cell (PEMFC). Our self-made hot embossing system can be used to fabricate assorted types of micro-channel structures on polymer substrates according to various applications, whose bottom width, top width, height and cross-sectional area vary in the aims of different situations. In order to obtain a high effective filling ratio, a new evaluation method is presented based on the four parameters of channel structures, and the Taguchi method is utilized to arrange three main factors (temperature, force and time) affecting the hot embossing in orthogonal arrays, quickly finding the optimal condition for the embossing process. The evaluation method for microchannels proposed in this paper, compared to traditional evaluation methods, incorporates the area factor, providing a more comprehensive assessment of the fabrication completeness of the microchannels. Additionally, it allows for the quick and simple identification of optimal conditions. The experimental results indicate that after determining the optimal embossing temperature, pressure and time using the Taguchi method, the effective filling rate remains above 95%, thereby enhancing the power density. Through variance analysis, it was found that temperature is the most significant factor affecting the hot embossing of microchannels. The high filling rate makes the process suitable for PEMFCs. The results demonstrate that under optimized process conditions, a self-made hot embossing system can effectively fabricate columnar structure microchannels for PEMFCs.
Persistence of Daptomycin-Resistant and Vancomycin-Resistant Enterococci in Hospitalized Patients with Underlying Malignancies: A 7-Year Follow-Up Study
Authors: El Haddad Lynn , Angelidakis Georgios , Zhai Yuting , Yaghi Layale , Arias Cesar A. , Shelburne Samuel A. , Jeong Kwangcheol Casey , Chemaly Roy F.
Published: August 2024

Vancomycin-resistant enterococci (VRE) commonly colonize the gut of individuals with hematologic malignancies or undergoing hematopoietic cell transplant (HCT) and may cause bacteremia. In 2012, we identified VRE isolates from patients and patients’ rooms and showed transmission networks of highly genetically related daptomycin-resistant (DR)-VRE strains. This is a follow-up study performing whole-genome sequencing (WGS) and phylogenetic analyses on 82 clinical VRE strains isolated from stools and blood cultures of patients with leukemia and HCT between 2015 and 2019. Here, we observed transmission of highly genetically related strains between rooms on the same or on different floors, including a DR-VRE strain identified in 2012. Eleven of twenty-eight patients with DR-VRE were never exposed to daptomycin, suggesting horizontal transmission. Fifteen of the twenty-eight patients with DR-VRE died within 30 days of positive blood cultures. Amongst those, one DR-VRE strain belonging to ST1471 had the virulence gene bopD responsible for biofilm formation. Additionally, to our knowledge, this is the first report of a DR-VRE strain belonging to ST323 in the United States. In summary, our study demonstrated the emergence and persistence of VRE strains, especially DR-VRE, in our hospital. Adding WGS to routine infection control measures may timely identify potential horizontal VRE transmission including multi-drug-resistant isolates.
Phytoplankton in Deep Lakes of the Dinaric Karst: Functional Biodiversity and Main Ecological Features
Authors: Hanžek Nikola , Šiljeg Mario , Šikić Tanja , Stanković Igor
Published: August 2024

Phytoplankton is a polyphyletic group of organisms that responds rapidly to environmental conditions and provides a reliable response to changes, making it a good ecological indicator for water quality monitoring. However, a gradient is almost essential for a reliable relationship between pressure and impact. In a low-gradient environment, ingenuity is required to outsmart the limitations of the commonly used linear relationship. Here, we examine changes in biomass and functional biodiversity by analysing larger data sets (2013–2022) in six ecologically diverse, natural, deep Croatian karst lakes with low nutrient gradients using nonlinear correlation coefficients and multivariate analyses in 209 samples. We found that phytoplankton biomass was most strongly influenced by nutrients, salinity and alkalinity, while light availability and total nitrogen strongly influenced phytoplankton functional biodiversity. An additional analysis of the TN:TP ratio revealed that the oligotrophic Lake Vransko is nitrogen-limited, and lakes Kozjak and Prošće are phosphorus-limited. This further clarified the relationship of phytoplankton to nutrients despite the low gradient. The complex analysis in this study provides a new perspective for predicting changes in the structure and succession of phytoplankton in deep karst lakes for successful management under apparent anthropogenic pressure and climate change.
Wheat Leaf Rust Fungus Effector Protein Pt1641 Is Avirulent to TcLr1
Authors: Chang Jiaying , Mapuranga Johannes , Li Ruolin , Zhang Yingdan , Shi Jie , Yan Hongfei , Yang Wenxiang
Published: August 2024

Wheat leaf rust fungus is an obligate parasitic fungus that can absorb nutrients from its host plant through haustoria and secrete effector proteins into host cells. The effector proteins are crucial factors for pathogenesis as well as targets for host disease resistance protein recognition. Exploring the role of effector proteins in the pathogenic process of Puccinia triticina Eriks. (Pt) is of great significance for unraveling its pathogenic mechanisms. We previously found that a cysteine-rich effector protein, Pt1641, is highly expressed during the interaction between wheat and Pt, but its specific role in pathogenesis remains unclear. Therefore, this study employed techniques such as heterologous expression, qRT-PCR analysis, and host-induced gene silencing (HIGS) to investigate the role of Pt1641 in the pathogenic process of Pt. The results indicate that Pt1641 is an effector protein with a secretory function and can inhibit BAX-induced programmed cell death in Nicotiana benthamiana. qRT-PCR analyses showed that expression levels of Pt1641 were different during the interaction between the high-virulence strain THTT and low-virulence strains FGD and Thatcher, respectively. The highest expression level in the low-virulence strain FGD was four times that of the high-virulence strain THTT. The overexpression of Pt1641 in wheat near-isogenic line TcLr1 induced callose deposition and H2O2 production on TcLr1. After silencing Pt1641 in the Pt low-virulence strain FGD on wheat near-isogenic line TcLr1, the pathogenic phenotype of Pt physiological race FGD on TcLr1 changed from “;” to “3”, indicating that Pt1641 plays a non-toxic function in the pathogenicity of FGD to TcLr1. This study helps to reveal the pathogenic mechanism of wheat leaf rust and provides important guidance for the mining and application of Pt avirulent genes.
Morphological Seed Traits Predict Early Performance of Native Species to Pelletized Seed Enhancement Technologies
Authors: Andres Samantha E. , Lieurance Paige E. , Mills Charlotte H. , Tetu Sasha G. , Gallagher Rachael V.
Published: August 2024

Native seeds are a finite resource, and their inclusion in revegetation is vital for supporting restoration outcomes that are both effective and scalable. Pelletized seed enhancement technologies (SETs) offer a promising solution to improve seed use efficiency in ecological restoration. Yet, knowledge of how diverse suites of native species perform when pelletized is required to optimize the application of SETs to the many species and ecosystems where restoration is required. Using a greenhouse trial of 64 Australian plant species, we assessed species performance to pelleting by evaluating (1) overall species amenability to pelleting based on total emergence and survival and (2) how pelleting modifies the rate of emergence based on average time to emergence, emergence rate index, and time spread of emergence. We investigated the potential for using morphological seed traits (seed endosperm:seed ratio, seed length, seed area, and seed coat thickness) to predict performance outcomes, by identifying traits that may aid in the prediction of species amenability to pelleting and emergence speed when pelletized. We found that some species demonstrate high amenability to pelleting and that pelleting can modify the emergence rates for many species. This work advances our understanding of the applicability of SETs for diverse native species, demonstrating the application of such technologies for meeting ecological restoration goals.