bluuebunny/crossref_metadata_2025 huggingface dataset:
Change in Pediatric Health Care Spending and Drug Utilization during the COVID-19 Pandemic
Authors: Lubrano Riccardo , Del Giudice Emanuela , Marcellino Alessia , Ventriglia Flavia , Dilillo Anna , De Luca Enrica , Mallardo Saverio , Isoldi Sara , Martucci Vanessa , Sanseviero Mariateresa , Iorfida Donatella , Malvaso Concetta , Cerimoniale Giovanni , Ragni Giuseppina , Grandinetti Anna Lisa , Arenare Loredana , Bloise Silvia
Published: December 2021

Objective: To evaluate how the restrictive measures implemented during the SARS-CoV-2 pandemic have influenced the incidence of the most common children’s diseases and the consumption of medications in 2020 compared to 2019. Methods: We involved all family pediatricians of the local health authority of Latina, from which we requested data of monthly visits in 2019 and 2020 for six common diseases disseminated through droplets and contact, and the territorial and integrative pharmaceutical unit of the area, from which we requested data of the net expenditure regarding the most commonly used drugs at pediatric age. Results: There was significant reduction in the incidence of the evaluated diseases and in the consumption of investigated drugs between 2019 and 2020 in the months when the restrictive measures were in place, with an attenuation of this effect during the months of the gradual loosening of those measures. Conclusion: Nonpharmaceutical intervention measures have caused changes in the diffusion of common pediatric diseases. We believe that the implementation of a reasonable containment strategy, even outside of the pandemic, could positively influence the epidemiology of infectious and allergic diseases in children, and healthcare system spending.
The Arabic Version of the Faces Pain Scale-Revised: Cultural Adaptation, Validity, and Reliability Properties When Used with Children and Adolescents
Authors: Finianos Jessica , Sánchez-Rodríguez Elisabet , Miró Jordi
Published: December 2021

The Faces Pain Scale-Revised (FPS-R) is widely used to assess pain intensity in young people. The aims of this research were to study the convergent and discriminant validity and reliability properties of a culturally adapted version of the FPS-R for its use with Arabic-speaking individuals. The sample consisted of 292 students living in Lebanon. They were interviewed online, asked to imagine themselves in one of two given situations based on their age (8–12 and 13–18 years old), and then asked rate the intensity of pain they would experience using the FPS-R-Arabic and a Numerical Rating Scale (NRS-11-Arabic). They were also asked to respond to the Pain Catastrophizing Scale (PCS-C-Arabic). Two weeks later, participants were asked to repeat the same procedure. The data showed strong associations between the scores of the FPS-R-Arabic and NRS-11-Arabic (r = 0.72; p < 0.001), which were higher than the associations of the scores of the FPS-Arabic with the PCS-C-Arabic scores (z = 7.36, p < 0.001). The associations between the FPS-R-Arabic scores on the two measurements were also strong (r = 0.76; p < 0.001). The findings support the convergent and discriminant validity and reliability of the FPS-R-Arabic scores when used to measure pain intensity in young people aged 8 to 18 years old.
Anthropometric Indices of Giardia-Infected Under-Five Children Presenting with Moderate-to-Severe Diarrhea and Their Healthy Community Controls: Data from the Global Enteric Multicenter Study
Authors: Shaima Shamsun Nahar , Das Sumon Kumar , Ahmed Shahnawaz , Jahan Yasmin , Khan Soroar Hossain , Mamun Gazi Md. Salahuddin , Shahid Abu Sadat Mohammad Sayeem Bin , Parvin Irin , Ahmed Tahmeed , Faruque A. S. G. , Chisti Mohammod Jobayer
Published: December 2021

Among all intestinal parasitosis, giardiasis has been reported to be associated with delayed growth in malnourished children under 5 living in low- and middle-income countries. Relevant data on the nutritional status of children aged 0–59 months presenting with moderate-to-severe diarrhea (MSD) and giardia infection were collected from sentinel health facilities of the Global Enteric Multicenter Study’s (GEMS) seven field settings, placed in diverse countries of Sub-Saharan Africa and South Asia between, December 2007 and February 2011. Then, this study analyzed a robust dataset of study participants (n = 22,569). Children having giardiasis with MSD constituted as cases (n = 1786), and those without MSD constituted as controls (n = 3470). Among the seven field sites, symptomatic giardiasis was 15% and 22% in Asian and African sites, respectively, whereas asymptomatic giardia infection (healthy without MSD) in Asian and African sites was 21.7% and 30.7%, respectively. Wasting and underweight were more frequently associated and stunting less often associated with symptomatic giardiasis (for all, p < 0.001). Symptomatic giardiasis had a significant association with worsening of nutritional status in under-five children. Improved socio-economic profile along with proper sanitation and hygienic practices are imperative to enhance child nutritional status, particularly in resource limited settings.
The Incredible and Sad Story of Boca de Cachón: How a Rural Community in the Hispaniola Is in a Prolonged, Heartless, and Predictable Climate Crisis
Authors: Gomez-Valenzuela Victor , Ramirez Katerin , Bonilla-Duarte Solhanlle
Published: December 2021

This article aims to briefly review the socio-economic impact caused by the flooding of Lake Enriquillo on the inhabitants of Boca Cachón due to the complex local phenomenon related to climate variability. Between 2003 and 2014, Boca de Cachón and the communities surrounding Lake Enriquillo were deeply affected by flooding of the Lake’s rising waters. Lake Enriquillo is the largest wetland in the Caribbean and the first designated RAMSAR site. In turn, Boca de Cachón could be considered the first human settlement formally displaced because of climate variability in the Dominican Republic and probably one of the first in the Americas in the twenty-first century. Boca de Cachón is a rural Municipal District located to the northwest of the municipality of Jimaní, with a population of around 3000 inhabitants on the southwest border with the Republic of Haiti and located in the Biosphere Reserve Jaragua-Bahoruco-Enriquillo. Given the future climatic scenarios for the Dominican Republic and the possible climate change that could exacerbate by excess or, by default, the socio-environmental problems in the Lake’s belt, it is necessary to support the communities in their capacity-building processes. The lessons learned from Boca de Cachón can serve as a learning space for adaptation processes in rural environments in the Caribbean region.
The Mechanical Properties and Elastic Anisotropy of η′-Cu6Sn5 and Cu3Sn Intermetallic Compounds
Authors: Ding Chao , Wang Jian , Liu Tianhan , Qin Hongbo , Yang Daoguo , Zhang Guoqi
Published: December 2021

Full intermetallic compound (IMC) solder joints present fascinating advantages in high-temperature applications. In this study, the mechanical properties and elastic anisotropy of η′-Cu6Sn5 and Cu3Sn intermetallic compounds were investigated using first-principles calculations. The values of single-crystal elastic constants, the elastic (E), shear (G), and bulk (B) moduli, and Poisson’s ratio (ν) were identified. In addition, the two values of G/B and ν indicated that the two IMCs were ductile materials. The elastic anisotropy of η′-Cu6Sn5 was found to be higher than Cu3Sn by calculating the universal anisotropic index. Furthermore, an interesting discovery was that the above two types of monocrystalline IMC exhibited mechanical anisotropic behavior. Specifically, the anisotropic degree of E and B complied with the following relationship: η′-Cu6Sn5 > Cu3Sn; however, the relationship was Cu3Sn > η′-Cu6Sn5 for the G. It is noted that the anisotropic degree of E and G was similar for the two IMCs. In addition, the anisotropy of the B was higher than the G and E, respectively, for η′-Cu6Sn5; however, in the case of Cu3Sn, the anisotropic degree of B, G, and E was similar.
Component Endoparasite Communities Mirror Life-History Specialization in Syntopic Reed Frogs (Hyperolius spp.)
Authors: Sinsch Ulrich , Balczun Carsten , Scheid Patrick , Dehling Jonas Maximilian
Published: December 2021

Most of our knowledge on the processes structuring parasite communities in amphibians originate from temperate-zone taxa, whereas Afrotropical communities have been neglected so far. We found evidence that ecological fitting of the hosts and, probably, differential immune response may influence the variation in parasite species richness, prevalence, and infestation intensity of East African frogs Hyperolius kivuensis and H. viridiflavus. The most closely related host species share the same macrohabitat (that implies the same pool of potential parasites), but differ in microhabitat preference, so that a comparative analyses of syntopic and allopatric populations is expedient to reveal ecological fitting. We detected 11 parasite species (one annelid, four nematodes, five trematodes, one cestode) and two endocommensal species (protozoans). The component parasite communities included 4–5 helminth species in H. kivuensis and 6–8 in the more aquatic H. viridiflavus, supporting the hypothesis that trematode diversity increases with the amount of time spent in water. Five parasite species (Orneoascaris chrysanthemoides, Clinostomum chabaudi, an undetermined echinostomatid) and two protozoans (Nyctotheroides sp., and Protoopalina sp.) are shared among the syntopic amphibian populations. This finding indicates a similar susceptibility of these amphibians to infestation from the local parasite pool. Yet, the low prevalence of single- and multi-species infestations in H. kivuensis indicates that parasite clearing by its immune response is probably more effective and prominent than in H. viridiflavus. Therefore, H. viridiflavus suffered from significantly reduced short-term survival due to the infection. Thus, we conclude that the processes structuring component parasite communities in amphibians do not differ generally between temperate-zone and Afrotropical host species, but they do in the magnitude of ecological fitting.
A Characterization of European Collective Action Initiatives and Their Role as Enablers of Citizens’ Participation in the Energy Transition
Authors: Lupi Veronica , Candelise Chiara , Calull Merce Almuni , Delvaux Sarah , Valkering Pieter , Hubert Wit , Sciullo Alessandro , Ivask Nele , van der Waal Esther , Iturriza Izaskun Jimenez , Paci Daniele , Della Valle Nives , Koukoufikis Giorgos , Dunlop Tessa
Published: December 2021

This paper provides novel additional evidence on the characteristics of Collective Action Initiatives (CAIs), investigating their role within the European energy sector. It analyses and presents results of a survey administered in six European countries: the Netherlands, Belgium, Italy, Poland, Estonia, and Spain. CAIs are studied in light of four key dimensions, those being their creation dynamics, the way they are organized, financed, and the activities they undertake. The results presented are also interpreted to reflect on their role as drivers of social innovation (SI) within energy transition in Europe. The analysis shows that the contribution of CAIs to the energy transition has a much wider scope than the development of energy projects and provision of energy services. CAIs are intrinsically socially innovative models of implementation as characterised by a strong level of citizen involvement and participation. Moreover, they have a potential multi-level role in the energy transition, from the technological and social perspectives. Indeed, alongside traditional energy activities, our results show that CAIs are evolving and expanding towards socially innovative activities, raising awareness on environmental issues, promoting citizens’ mobilization, and fostering social inclusion.
Impact of Hydrogen Injection on Natural Gas Measurement
Authors: Dell’Isola Marco , Ficco Giorgio , Moretti Linda , Jaworski Jacek , Kułaga Paweł , Kukulska–Zając Ewa
Published: December 2021

Hydrogen is increasingly receiving a primary role as an energy vector in ensuring the achievement of the European decarbonization goals by 2050. In fact, Hydrogen could be produced also by electrolysis of water using renewable sources, such as photovoltaic and wind power, being able to perform the energy storage function, as well as through injection into natural gas infrastructures. However, hydrogen injection directly impacts thermodynamic properties of the gas itself, such as density, calorific value, Wobbe index, sound speed, etc. Consequently, this practice leads to changes in metrological behavior, especially in terms of volume and gas quality measurements. In this paper, the authors present an overview on the impact of hydrogen injection in natural gas measurements. In particular, the changes in thermodynamic properties of the gas mixtures with different H2 contents have been evaluated and the effects on the accuracy of volume conversion at standard conditions have been investigated both on the theoretical point of view and experimentally. To this end, the authors present and discuss the effect of H2 injection in gas networks on static ultrasonic domestic gas meters, both from a theoretical and an experimental point of view. Experimental tests demonstrated that ultrasonic gas meters are not significantly affected by H2 injection up to about 10%.
Impact of a Synthetic Component on the Emission of Volatile Organic Compounds during the Combustion Process in a Miniature Turbine Engine
Authors: Gawron Bartosz , Górniak Aleksander , Białecki Tomasz , Janicka Anna , Włostowski Radosław , Włóka Adriana , Molska Justyna , Zawiślak Maciej
Published: December 2021

This paper refers to the study of biofuel as an alternative power source for turbine aviation engines. Blends of Jet A-1 fuel and synthesized hydrocarbons from Hydrotreated Esters and Fatty Acids (HEFA) technology at different proportions, such as 25%, 50% and 75%, were used for tests. All the test results were compared with the neat Jet A-1 fuel. A miniature GTM series turbojet engine was used in the test rig studies. During the tests conducted at a specific rotational speed, selected engine operating parameters as well as the emission of volatile organic compounds were measured. In terms of engine performance, no significant differences were found between the test fuels. The results of volatile organic compound emissions indicate that among the most toxic compounds the highest concentrations were obtained for benzene. The addition of the HEFA synthetic component and increasing its proportion in the blend resulted in the obtained concentration values for benzene showing a decreasing trend. The plotted utility profile indicates that the most optimal blend, i.e., the least toxic, is the blend with the share (v/v) of 62.5% of Jet A-1 fuel and 37.5% of HEFA component.
Energy Management Systems for Optimal Operation of Electrical Micro/Nanogrids
Authors: Di Piazza Maria Carmela
Published: December 2021

Energy management systems (EMSs) have been introduced in electrical power systems to optimize operations of the electrical grid infrastructure and to provide support to the grid operator in terms of optimized decisions [...]
Synergy Effect of Factors Characterising Village Transition Zones on Speed Reduction
Authors: Sołowczuk Alicja , Kacprzak Dominik
Published: December 2021

There are various traffic calming measures that can be installed in village transition zones. So far, focus was placed on diversified use of pavement markings, amounts of horizontal deflection, shape of the installed chicanes or central islands, presence of gateway, etc., and their location along the transition zone. However, the combined effect of the different transition zone factors on speed reductions has been rarely studied so far. Authors put forward a hypothesis of there being some determinants, which in combination influence speed reduction. To corroborate the hypothesis on the combined impact of the transition zone features on speed reduction in the village transition zones and to validate the established relationships the authors conducted verification study in transition zones containing chicanes or central islands. To verify this hypothesis the authors studied twenty transition zones and managed to confirm the hypothesis at 95% confidence level. The authors used previously adopted binary methods, verified the previously defined factors and added a few new determinants. The contribution of this study is a further investigation of the synergy effect of various relevant factors and the findings can assist in planning new transition zones or suggest additional measures to achieve the desired speed reduction in existing transition zones.
NOx and CO Fluctuations in a Busy Street Canyon
Authors: Brimblecombe Peter , Chu Meng-Yuan , Liu Chun-Ho , Ning Zhi
Published: December 2021

Busy street canyons can have a large flow of vehicles and reduced air exchange and wind speeds at street level, exposing pedestrians to high pollutant concentrations. The airflow tended to move with vehicles along the canyon and the 1-s concentrations of NO, NO2 and CO were highly skewed close to the road and more normally distributed at sensors some metres above the road. The pollutants were more autocorrelated at these elevated sensors, suggesting a less variable concentration away from traffic in the areas of low turbulence. The kerbside concentrations also showed cyclic changes approximating nearby traffic signal timing. The cross-correlation between the concentration measurements suggested that the variation moved at vehicle speed along the canyon, but slower vertically. The concentrations of NOx and CO were slightly higher at wind speeds of under a metre per second. The local ozone concentrations had little effect on the proportion of NOx present as NO2. Pedestrians on the roadside would be unlikely to exceed the USEPA hourly guideline value for NO2 of 100 ppb. Across the campaign period, 100 individual minutes exceeded the guidelines, though the effect of short-term, high-concentration exposures is not well understood. Tram stops at the carriageway divider are places where longer exposures to higher levels of traffic-associated pollutants are possible.
Features of the Hydrocarbon Distribution in the Bottom Sediments of the Norwegian and Barents Seas
Authors: Nemirovskaya Inna A. , Khramtsova Anastasia V.
Published: December 2021

The results of the study of hydrocarbons (HCs): aliphatic (AHCs) and polycyclic aromatic hydrocarbons (PAHs) in bottom sediments (2019 and 2020, cruises 75 and 80 of the R/V Akademik Mstislav Keldysh) in the Norwegian-Barents Sea basin: Mohns Ridge, shelf Svalbard archipelago, Sturfiord, Medvezhinsky trench, central part of the Barents Sea, Novaya Zemlya shelf, Franz Victoria trough are presented. It has been established that the organo-geochemical background of the Holocene sediments was formed due to the flow of sedimentary material in the coastal regions of the Barents Sea on shipping routes. The anthropogenic input of HCs into bottom sediments leads to an increase in their content in the composition of Corg (in the sandy sediments of the Kaninsky Bank at an AHC concentration up to 64 μg/g, when its proportion in the composition of Corg reaches 11.7%). The endogenous influence on the of the Svalbard archipelago shelf in Sturfiord and in the Medvezhinsky Trench determines the specificity of local anomalies in the content and composition of HCs. This is reflected in the absence of a correlation between HCs and the grain size composition of sediments and Corg content, as well as a change in hydrocarbon molecular markers. At the same time, the sedimentary section is enriched in light alkanes and naphthalene’s that may be due to emission during point discharge of gas fluid from sedimentary rocks of the lower stratigraphic horizons and/or sipping migration.
Profiles of Volatile and Phenolic Compounds as Markers of Ripening Stage in Candonga Strawberries
Authors: Cozzolino Rosaria , Pace Bernardo , Palumbo Michela , Laurino Carmine , Picariello Gianluca , Siano Francesco , De Giulio Beatrice , Pelosi Sergio , Cefola Maria
Published: December 2021

Volatile compounds, quality traits (total phenols and antioxidant capacity) and High-performance liquid chromatography (HPLC)-isolated polyphenols of strawberries, variety Sabrosa, commercially referred to as “Candonga”, harvested at three different times (H1, H2 and H3) and at two different ripening stages, namely half-red (Half-red-H1, Half-red-H2 and Half-red-H3) and red (Red-H1, Red-H2 and Red-H3) were evaluated. Dominant anthocyanins, namely cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside and pelargonidin-3-O-rutinoside, as well as p-coumaryl hexoside increased during harvesting, differently from flavonoids, such as quercetin-3-O-glucoside, kaempferol-3-O-glucoronide and quercetin 3-O-glucoronide, that declined. Samples clustered in different quadrants of the principal component analysis (PCA) performed on volatiles, quality traits and phenolic compounds, highlighting that only the red samples were directly correlated to volatile components, as volatiles clearly increased both in number and amount during ripening. In particular, volatiles with a positive impact on the consumers’ acceptance, including butyl butyrate, ethyl hexanoate, hexyl acetate, nonanal, terpenes and lactones, were positively associated with the Red-H1 and Red-H2 strawberries, while volatiles with negative coefficients related to consumer liking, including isopropyl butyrate, isoamyl butyrate and mesifurane directly correlated with the Red-H3 samples. Accordingly, strawberries harvested at Red-H1 and Red-H2 ripening stages could be preferred by the consumers compared to the Red-H3 fruit. Altogether, these results could help to individuate quality traits as putative markers of the ripening stage, and optimize the process of post-harvesting ripening to preserve or improve the desirable aromatic characteristics of strawberries.
Hapten Synthesis and Monoclonal Antibody Preparation for Simultaneous Detection of Albendazole and Its Metabolites in Animal-Origin Food
Authors: Shao Shibei , Zhou Xuping , Dou Leina , Bai Yuchen , Mi Jiafei , Yu Wenbo , Zhang Suxia , Wang Zhanhui , Wen Kai
Published: December 2021

Albendazole (ABZ) is one of the benzimidazole anthelmintics, and the overuse of ABZ in breeding industry can lead to drug resistance and a variety of toxic effects in humans. Since the residue markers of ABZ are the sum of ABZ and three metabolites (collectively referred to as ABZs), albendazole-sulfone (ABZSO2), albendazole-sulfoxide (ABZSO), and albendazole-2-amino-sulfone (ABZNH2SO2), an antibody able to simultaneously recognize ABZs with high affinity is in urgent need to develop immunoassay for screening purpose. In this work, an unreported hapten, 5-(propylthio)-1H-benzo[d]imidazol-2-amine, was designed and synthesized, which maximally exposed the characteristic sulfanyl group of ABZ to the animal immune system to induce expected antibody. One monoclonal antibody (Mab) that can simultaneously detect ABZs was obtained with IC50 values of 0.20, 0.26, 0.77, and 10.5 μg/L for ABZ, ABZSO2, ABZSO, and ABZNH2SO2 in ic-ELISA under optimized conditions respectively, which has been never achieved in previous reports. For insight into the recognition profiles of the Mab, we used computational chemistry method to parameterize cross-reactive molecules in aspects of conformation, electrostatic fields, and hydrophobicity, revealing that the hydrophobicity and conformation of characteristic group of molecules might be the key factors that together influence antibody recognition with analytes. Furthermore, the practicability of the developed ic-ELISA was verified by detecting ABZs in spiked milk, beef, and liver samples with recoveries of 60% to 108.8% and coefficient of variation (CV) of 1.0% to 15.9%.
The Connection between MicroRNAs and Oral Cancer Pathogenesis: Emerging Biomarkers in Oral Cancer Management
Authors: Osan Ciprian , Chira Sergiu , Nutu Andreea Mihaela , Braicu Cornelia , Baciut Mihaela , Korban Schuyler S. , Berindan-Neagoe Ioana
Published: December 2021

Oral cancer is a common human malignancy that still maintains an elevated mortality rate despite scientific progress. Tumorigenesis is driven by altered gene expression patterns of proto-oncogenes and tumor-suppressor genes. MicroRNAs, a class of short non-coding RNAs involved in gene regulation, seem to play important roles in oral cancer development, progression, and tumor microenvironment modulation. As properties of microRNAs render them stable in diverse liquid biopsies, together with their differential expression signature in cancer cells, these features place microRNAs at the top of promising biomarkers for diagnostic and prognostic values. In this review, we highlight eight expression levels and functions of the most relevant microRNAs involved in oral cancer development, progression, and microenvironment sustainability. Furthermore, we emphasize the potential of using these small RNA species as non-invasive biomarkers for the early detection of oral cancerous lesions. Conclusively, we highlight the perspectives and limitations of microRNAs as novel diagnostic tools, as well as therapeutic models.
Alcohol Abuse and Insomnia Disorder: Focus on a Group of Night and Day Workers
Authors: Plescia Fulvio , Cirrincione Luigi , Martorana Daniela , Ledda Caterina , Rapisarda Venerando , Castelli Valentina , Martines Francesco , Vinnikov Denis , Cannizzaro Emanuele
Published: December 2021

The sleep-wake cycle plays a fundamental role in maintaining the physiological balance of our body. Its alteration favours the genesis of several organic alterations and diseases including sleep disorders and the consumption of several substances of abuse. It has been reported that the work activity, especially that carried out during the night, is able to influence the sleep-wake cycle, promoting the development of insomnia, which, in turn, would subject the worker to a stressful condition such as to encourage adverse behaviour such as the use/abuse of psychotropic substances. Based on the above premises, the aim of our research was to evaluate, in night workers: (i) the pattern of consumption of alcoholic beverages; (ii) the presence of insomnia; and (iii) the possible correlation between alcohol consumption and insomnia disorder. We used the AUDIT-C test (the abbreviated version of the Alcohol Use Disorders Identification Test) and the Insomnia Severity Index to assess alcohol consumption and insomnia disorder, respectively. All questionnaires were completed by workers of both sexes belonging to different types of work activities, exclusively day or night. The results of our research show a higher propensity of night workers to consume alcoholic beverages than those who work during daytime hours, often in binge-drinking mode. In addition, an increase in the amount of alcohol consumed was found to be related to insomnia disorder, especially in night workers. This study provides further awareness of the importance of the negative impact of alcohol consumption on sleep quality in night workers.
Acculturation Strategies and Pap Screening Uptake among Sub-Saharan African Immigrants (SAIs)
Authors: Adegboyega Adebola , Wu Jia-Rong , Mudd-Martin Gia
Published: December 2021

Although regular cervical cancer screening can prevent cervical cancer, screening utilization remains low among immigrant population including sub-Saharan African immigrants (SAIs). Acculturation is a complex process, which can lead to adoption of positive or negative health behaviors from the dominant culture. Acculturation strategies are the varying ways in which individuals seek to go about their acculturation by either maintaining or rejecting their own cultural values ip or accepting or rejecting the host culture’s cultural values. Cervical cancer screening behaviors among SAI women may be influenced by their acculturation strategies. We conducted a secondary analysis of data to examine the relationship between acculturation strategies and Pap screening among 99 SAI women recruited from community settings. Data were collected on Pap screening behavior and acculturation strategy. Traditionalists and Integrationists were the dominant acculturation strategies; 32.3% women were Traditionalists and 67.7% Integrationists. From the logistic regression models, Integrationists had seven times the odds of having ever been screened compared to Traditionalists (OR = 7.08, 95% CI = 1.54–28.91). Cervical cancer screening interventions should prioritize Traditionalists for cancer screening. Acculturation strategies may be used to tailor cancer prevention and control for SAIs. More research among a larger SAI women sample is warranted to further our understanding of Pap screening patterns and acculturation strategies.
Risk Perception of Health Risks Associated with Radiation Exposure among Residents of Okuma, Fukushima Prefecture
Authors: Oishi Keiko , Orita Makiko , Taira Yasuyuki , Kashiwazaki Yuya , Matsunaga Hitomi , Takamura Noboru
Published: December 2021

Ten years have passed since the Great East Japan Earthquake and the subsequent Fukushima Daiichi nuclear power plant accident on 11 March 2011. Okuma is a town hosting the Fukushima Daiichi nuclear power plant. The evacuation order for Okuma was partially lifted in April 2019. To clarify factors associated with risk perceptions of radiation among the residents of Okuma, we conducted a questionnaire survey in January 2021. Our results revealed that resident anxieties regarding the health effects of radiation exposure from living in Okuma were independently associated with positive PCL-Specific score, recognition of the consultation services with radiation experts in the municipal government of Okuma, and planned request for consultation service regarding radiation exposure by radiation experts, along with being female and living with a child. It is important for radiation experts to promote periodic communication of risks with individuals on a small scale to provide accurate information about the health effects of radiation and to provide maternal and child healthcare services and support regarding child-rearing and radiation exposure, to reduce concerns about radiation exposure and facilitate healthy living and wellbeing in Okuma.
Comparison of Digital Self-Assessment Systems and Faculty Feedback for Tooth Preparation in a Preclinical Simulation
Authors: Stoilov Milan , Trebess Lea , Klemmer Markus , Stark Helmut , Enkling Norbert , Kraus Dominik
Published: December 2021

Background: Regarding the new dental licensing regulations in Germany (AOZ), this study evaluated the effectiveness of two different digital tooth preparation validation systems in comparison to traditional faculty feedback. Methods: Participants were randomly divided into groups: Faculty Feedback (FF: n = 33), PrepCheck® (PC: n = 32) and Dental Teacher™ (n = 32). Students had the task to prepare tooth 16 for a retentive full-cast crown. Preparations could be repeated as often as desired. Feedback was provided either by faculty staff or by digital validation systems only. Exams were conducted and graded by two independent and experienced examiners. A survey was performed to evaluate the assessment concepts. Results: No statistical difference in examination performance between groups could be observed. Nevertheless, the survey showed participants preferred consulting the faculty staff rather than the digital validation systems. Students preferred practising with DT rather than with PC. Conclusions: Although both classical and digital methods showed comparable results regarding the preparation examination performance, direct feedback by the faculty staff is still appreciated by the students. A combination of both methods is mandatory since demonstration and advice by the teacher is needed. However, digital tooth preparation validation systems are predestined for free practice sessions, providing self-assessment.
Impact of the COVID-19 Pandemic on Adolescents’ Sexual and Reproductive Health in Low- and Middle-Income Countries
Authors: Meherali Salima , Adewale Bisi , Ali Sonam , Kennedy Megan , Salami Bukola (Oladunni) , Richter Solina , Okeke-Ihejirika Phil E. , Ali Parveen , da Silva Kênia Lara , Adjorlolo Samuel , Aziato Lydia , Kwankye Stephen Owusu , Lassi Zohra
Published: December 2021

Adolescents living in low- and middle-income countries (LMICs) are struggling with accessing sexual and reproductive health (SRH) services, and COVID-19 has escalated the problem. The purpose of this review was to identify and assess the existing literature on the impact of the pandemic on SRH needs and access to services by adolescents in LMICs. A scoping review was conducted to collate findings on the topic. Searches were performed on eight databases. Data were extracted and categorized into various themes. After removing duplicates and performing a full-text reading of all articles, nine articles were included in our review. Our findings generated several themes related to adolescents’ sexual and reproductive health during the COVID-19 pandemic. These include (1) limited access to sexual and reproductive health services, (2) school closure and increased rate of early marriages, (3) sexual or intimate partner violence during COVID-19, (4) disruption in maternity care, (5) adolescents’ involvement in risky or exploitative work, (6) intervention to improve sexual and reproductive health services during COVID-19, and (7) policy development related to adolescent sexual and reproductive health. Several recommendations were made on policies—for instance, the use of telemedicine and community-based programs as a way to deliver SRH services to adolescents during and after a pandemic.
The Influence of Noise in the Neurofeedback Training Sessions in Student Athletes
Authors: Domingos Christophe , da Silva Caldeira Higino , Miranda Marco , Melício Fernando , Rosa Agostinho C. , Pereira José Gomes
Published: December 2021

Considering that athletes constantly practice and compete in noisy environments, the aim was to investigate if performing neurofeedback training in these conditions would yield better results in performance than in silent ones. A total of forty-five student athletes aged from 18 to 35 years old and divided equally into three groups participated in the experiment (mean ± SD for age: 22.02 ± 3.05 years). The total neurofeedback session time for each subject was 300 min and were performed twice a week. The environment in which the neurofeedback sessions were conducted did not seem to have a significant impact on the training’s success in terms of alpha relative amplitude changes (0.04 ± 0.08 for silent room versus 0.07 ± 0.28 for noisy room, p = 0.740). However, the group exposed to intermittent noise appears to have favourable results in all performance assessments (p = 0.005 for working memory and p = 0.003 for reaction time). The results of the study suggested that performing neurofeedback training in an environment with intermittent noise can be interesting to athletes. Nevertheless, it is imperative to perform a replicated crossover design.
Factors Influencing Adolescent Anxiety: The Roles of Mothers, Teachers and Peers
Authors: Chen Xin , Li Mengge , Gong Huoliang , Zhang Zekun , Wang Wei
Published: December 2021

Grounded in social–ecological system theory, the present study tested the mediating effects of maternal psychological flexibility and mother–adolescent attachment on the relationship between maternal adult attachment and adolescent anxiety as well as the moderating effects of teacher support and peer support on the relationship between mother–adolescent attachment and adolescent anxiety. In total, 1139 Chinese mothers and adolescents completed a set of questionnaires, including the Experiences in Close Relationships Scale, Parental Psychological Flexibility Questionnaire, Inventory of Parent and Peer Attachment, Trait Anxiety Inventory, and Multidimensional Scale of Perceived Social Support. The results revealed that maternal adult attachment had a positive impact on adolescent anxiety. The relationship between maternal adult attachment and adolescent anxiety was chain mediated by maternal psychological flexibility and mother–adolescent attachment. In addition, teacher support and peer support had moderating effects on the relationship between mother–adolescent attachment and adolescent anxiety. These findings support the systematic social ecosystem perspective and highlight the differences in the effects of different maternal adult attachment styles, teacher support, and peer support on adolescent anxiety.
A Health Belief Model-Based Motivational Interviewing for Medication Adherence and Treatment Success in Pulmonary Tuberculosis Patients
Authors: Parwati Ni Made , Bakta I Made , Januraga Pande Putu , Wirawan I Made Ady
Published: December 2021

Medication adherence behavior plays a central role in the success of tuberculosis (TB) treatment. Conventional motivation is not optimal in strengthening long-term medication adherence. A motivational interviewing (MI) communication motivation model based on the Health Belief Model (HBM) was designed with the main objective of improving medication adherence and treatment success. This study used an experimental design with a randomized posttest-only control group design. The intervention and control groups consisted of 107 TB patients each, who were selected by random cluster sampling. The study was conducted from November 2020 to June 2021 at 38 public health centers in Bali Province. The HBM-based MI model intervention was given in seven counseling sessions, pill count percentages were used to measure medication adherence, and treatment success was based on sputum examination results. Logistic regression was used to assess the effect of the intervention on medication adherence and treatment success. Logistic regression analysis showed that MI-based HBM and knowledge were the most influential variables for increasing medication adherence and treatment success. Medication adherence was 4.5 times greater (ARR = 4.51, p = 0.018) and treatment success was 3.8 times greater (ARR = 3.81, p < 0.038) in the intervention group compared to the control group, while the secondary outcome of knowledge of other factors together influenced medication adherence and treatment success. The conclusion is that the HBM-based MI communication motivation model creates a patient-centered relationship by overcoming the triggers of treatment barriers originating from the HBM construct, effectively increasing medication adherence and treatment success for TB patients, and it needs further development by involving families in counseling for consistent self-efficacy of patients in long-term treatment.
“I Teach, Therefore I Am”: The Serial Relationship between Perceived Vulnerability to Disease, Fear of COVID-19, Teacher Identification and Teacher Satisfaction
Authors: Padmanabhanunni Anita , Pretorius Tyrone
Published: December 2021

In early 2020, school closures were implemented globally to curb the spread of the COVID-19 pandemic. In South Africa, emergency remote teaching was not sustainable, and conventional teaching resumed in the context of the second and third waves of the pandemic, heightening fear and anxiety about infection among teachers. The pandemic necessitated shifts in the scope of a teacher’s job, potentially impacting their professional identity and job satisfaction. This study investigated the interrelationship between teaching identification, teaching satisfaction, fear of COVID-19 and perceived vulnerability to disease among a sample of South African school teachers (n = 355). A serial mediation analysis supported the hypotheses that teaching identification mediated both the relationship between fear of COVID-19 and teacher satisfaction and the association between perceived vulnerability to disease, fear of COVID-19 and teacher satisfaction. The findings suggest that teacher identification is a potential protective factor, and strengthening professional identification can potentially assist teachers as they negotiate the uncertainty and stress associated with the current pandemic.
Identification of Novel Therapeutic Targets for Polyglutamine Diseases That Target Mitochondrial Fragmentation
Authors: Traa Annika , Machiela Emily , Rudich Paige D. , Soo Sonja K. , Senchuk Megan M. , Van Raamsdonk Jeremy M.
Published: December 2021

Huntington’s disease (HD) is one of at least nine polyglutamine diseases caused by a trinucleotide CAG repeat expansion, all of which lead to age-onset neurodegeneration. Mitochondrial dynamics and function are disrupted in HD and other polyglutamine diseases. While multiple studies have found beneficial effects from decreasing mitochondrial fragmentation in HD models by disrupting the mitochondrial fission protein DRP1, disrupting DRP1 can also have detrimental consequences in wild-type animals and HD models. In this work, we examine the effect of decreasing mitochondrial fragmentation in a neuronal C. elegans model of polyglutamine toxicity called Neur-67Q. We find that Neur-67Q worms exhibit mitochondrial fragmentation in GABAergic neurons and decreased mitochondrial function. Disruption of drp-1 eliminates differences in mitochondrial morphology and rescues deficits in both movement and longevity in Neur-67Q worms. In testing twenty-four RNA interference (RNAi) clones that decrease mitochondrial fragmentation, we identified eleven clones—each targeting a different gene—that increase movement and extend lifespan in Neur-67Q worms. Overall, we show that decreasing mitochondrial fragmentation may be an effective approach to treating polyglutamine diseases and we identify multiple novel genetic targets that circumvent the potential negative side effects of disrupting the primary mitochondrial fission gene drp-1.
Effects of Heme Oxygenase-1 on c-Kit-Positive Cardiac Cells
Authors: Li Qianhong , Dasari Chandrashekhar , Li Ding , Arshia Asma , Umer Ahmed Muaaz , Abouzid Mohamed Riad Abdelgawad , Guo Yiru , Bolli Roberto
Published: December 2021

Heme oxygenase-1 (HO-1) is one of the most powerful cytoprotective proteins known. The goal of this study was to explore the effects of HO-1 in c-kit-positive cardiac cells (CPCs). LinNEG/c-kitPOS CPCs were isolated and expanded from wild-type (WT), HO-1 transgenic (TG), or HO-1 knockout (KO) mouse hearts. Compared with WT CPCs, cell proliferation was significantly increased in HO-1TG CPCs and decreased in HO-1KO CPCs. HO-1TG CPCs also exhibited a marked increase in new DNA synthesis during the S-phase of cell division, not only under normoxia (21% O2) but after severe hypoxia (1% O2 for 16 h). These properties of HO-1TG CPCs were associated with nuclear translocation (and thus activation) of Nrf2, a key transcription factor that regulates antioxidant genes, and increased protein expression of Ec-SOD, the only extracellular antioxidant enzyme. These data demonstrate that HO-1 upregulates Ec-SOD in CPCs and suggest that this occurs via activation of Nrf2, which thus is potentially involved in the crosstalk between two antioxidants, HO-1 in cytoplasm and Ec-SOD in extracellular matrix. Overexpression of HO-1 in CPCs may improve the survival and reparative ability of CPCs after transplantation and thus may have potential clinical application to increase efficacy of cell therapy.
Epigenetic Age Acceleration Is Not Associated with Age-Related Macular Degeneration
Authors: Saptarshi Neil , Green Daniel , Cree Angela , Lotery Andrew , Paraoan Luminita , Porter Louise F.
Published: December 2021

DNA methylation age (DNAm age) estimation is a powerful biomarker of human ageing. To date, epigenetic clocks have not been evaluated in age-related macular degeneration (AMD). Here, we perform genome-wide DNA methylation analyses in blood of AMD patients with a documented smoking history (14 AMD, 16 Normal), identifying loci of differential methylation (DML) with a relaxed p-value criterion (p ≤ 10−4). We conduct DNAm age analyses using the Horvath-multi tissue, Hannum and Skin & Blood epigenetic clocks in both blood and retinal pigment epithelium (RPE). We perform Ingenuity Pathway Analysis Causal Network Analysis (IPA CNA) on the topmost significantly differentially methylated CpG probes in blood and RPE. Results show poor performance of epigenetic clocks in RPE. Epigenetic age acceleration (EAA) was not observed in AMD. However, we observe positive EAA in blood of smokers, and in smokers with AMD. DML analysis revealed hypomethylation at cg04953735 within RPTOR (p = 6.51 × 10−5; Δβ = −11.95%). IPA CNA in the RPE also identified RPTOR as the putative master regulator, predicted to be inhibited in AMD. In conclusion, this is the first study evaluating an association of epigenetic ageing in AMD. We posit a role for RPTOR as a common master regulator of methylation changes in the RPE in AMD.
Hypothalamic Regulation of Obesity
Authors: Rodríguez-Rodríguez Rosalía , Miralpeix Cristina
Published: December 2021

Obesity has now reached pandemic proportions and represents a major socioeconomic and health problem in our societies [...]
4,6-Dichloro-5-Nitrobenzofuroxan: Different Polymorphisms and DFT Investigation of Its Reactivity with Nucleophiles
Authors: Chugunova Elena , Akylbekov Nurgali , Dobrynin Alexey , Burilov Alexander , Boga Carla , Micheletti Gabriele , Frenna Vincenzo , Mattioli Edoardo Jun , Calvaresi Matteo , Spinelli Domenico
Published: December 2021

This research focuses on the X-ray structure of 4,6-dichloro-5-nitrobenzofuroxan 1 and of some of its amino derivatives (4a, 4e, 4g, and 4l) and on DFT calculations concerning the nucleophilic reactivity of 1. We have found that by changing the solvent used for crystallization, it is possible to obtain 4,6-dichloro-5-nitrobenzofuroxan (1) in different polymorphic structures. Moreover, the different torsional angles observed for the nitro group in 1 and in its amino derivatives (4a, 4e, 4g, and 4l) are strictly dependent on the steric hindrance of the substituent at C-4. DFT calculations on the course of the nucleophilic substitution confirm the role of the condensed furoxan ring in altering the aromaticity of the carbocyclic frame, while chlorine atoms strongly influence the dihedral angle and the rotational barrier of the nitro group. These results corroborate previous observations based on experimental kinetic data and give a deep picture of the reaction with amines, which proceeds via a “non-aromatic” nucleophilic substitution.
Function of Chloroplasts in Plant Stress Responses
Authors: Song Yun , Feng Li , Alyafei Mohammed Abdul Muhsen , Jaleel Abdul , Ren Maozhi
Published: December 2021

The chloroplast has a central position in oxygenic photosynthesis and primary metabolism. In addition to these functions, the chloroplast has recently emerged as a pivotal regulator of plant responses to abiotic and biotic stress conditions. Chloroplasts have their own independent genomes and gene-expression machinery and synthesize phytohormones and a diverse range of secondary metabolites, a significant portion of which contribute the plant response to adverse conditions. Furthermore, chloroplasts communicate with the nucleus through retrograde signaling, for instance, reactive oxygen signaling. All of the above facilitate the chloroplast’s exquisite flexibility in responding to environmental stresses. In this review, we summarize recent findings on the involvement of chloroplasts in plant regulatory responses to various abiotic and biotic stresses including heat, chilling, salinity, drought, high light environmental stress conditions, and pathogen invasions. This review will enrich the better understanding of interactions between chloroplast and environmental stresses, and will lay the foundation for genetically enhancing plant-stress acclimatization.