bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of menstrual pain and symptoms on activities of daily living and work absenteeism: a cross-sectional study
Authors: Leon-Larios Fatima , Silva-Reus Isabel , Puente Martínez María José , Renuncio Roba Abel , Ibeas Martínez Eva , Lahoz Pascual Isabel , Naranjo Ratia Maria Cassia , Quílez Conde José Cruz
Published: February 2024

Objective

To examine the prevalence of menstrual pain among women of reproductive age and its impact on their daily lives and professional responsibilities.

Methods

A cross-sectional and descriptive study was conducted in July and August 2022. Phone interviews were carried out using a random system to select women aged between 15 and 49 years old. The questionnaire included sociodemographic variables, contraception method used, characteristics of the menstrual pattern (pain and bleeding amount), its influence on their working life, and if they would need to resort to sick leaves due to the impairments arising from the menstrual symptoms.

Results

A total of 1800 women representative of the Spanish population took part in this study. 72.6% of them report menstrual pain, with 45.9% requiring medication. 35.9% identify their menstrual bleeding as intense or very intense. 38.8% assert that menstrual discomforts affect their everyday life. 34.3% would have required not attending their work activities or having requested sick leave due to the discomforts, although only 17.3% of the women finally requested so, mainly because 58.4% considered that it might imply consequences in their professional environment, especially those with Higher Education. The women who report more discomfort are the youngest ones and those who resort to condoms as a contraceptive method (p < 0.001).

Conclusions

Menstrual pain is a prevalent problem among women of reproductive age and can affect their everyday life and professional environment, requiring work leaves on some occasions.
Periostin/Bone Morphogenetic Protein 1 axis axis regulates proliferation and osteogenic differentiation of sutured mesenchymal stem cells and affects coronal suture closure in the TWIST1+/− mouse model of craniosynostosis
Authors: Feng ShuBin , Feng Qiang , Dong LiuJian , Lv Qiang , Mei ShiYue , Zhang YaoDong
Published: February 2024

Background and objective

The pathogenesis of coronal suture craniosynostosis is often attributed to the dysregulated cellular dynamics, particularly the excessive proliferation and abnormal osteogenic differentiation of suture cells. Despite its clinical significance, the molecular mechanims of this condition remain inadequately understood. This study is dedicated to exploring the influence of the Periostin/Bone Morphogenetic Protein 1 (BMP1) axis on the growth and osteogenic maturation of Suture Mesenchymal Stem Cells (SMSCs), which are pivotal in suture homeostasis.

Methods

Neonatal TWIST Basic Helix-Loop-Helix Transcription Factor 1 heterozygous (TWIST1+/−) mice, aged one day, were subjected to adenoviral vector-mediated Periostin upregulation. To modulate Periostin/BMP1 levels in SMSCs, we employed siRNA and pcDNA 3.1 vectors. Histological and molecular characterizations, including hematoxylin and eosin staining, Western blot, and immunohistochemistry were employed to study suture closure phenotypes and protein expression patterns. Cellular assays, encompassing colony formation, 5-ethynyl-2'deoxyuridine, and wound healing tests were conducted to analyze SMSC proliferation and migration. Osteogenic differentiation was quantified using Alkaline Phosphatase (ALP) and Alizarin Red S (ARS) staining, while protein markers of proliferation and differentiation were evaluated by Western blotting. The direct interaction between Periostin and BMP1 was validated through co-immunoprecipitation assays.

Results

In the TWIST1+/− model, an upregulation of Periostin coupled with a downregulation of BMP1 was observed. Augmenting Periostin expression mitigated craniosynostosis. In vitro, overexpression of Periostin or BMP1 knockdown suppressed SMSC proliferation, migration, and osteogenic differentiation. Periostin knockdown manifested an inverse biological impact. Notably, the suppressive influence of Periostin overexpression on SMSCs was effectively counteracted by upregulating BMP1. There was a direct interaction between Periostin and BMP1.

Conclusion

These findings underscore the significance of the Periostin/BMP1 axis in regulating craniosynostosis and SMSC functions, providing new insights into the molecular mechanisms of craniosynostosis and potential targets for therapeutic intervention.
Several first-line anti-hypertensives act on fibrosarcoma progression and PD1ab blockade therapy
Authors: Sun Jianwen , Zhang Chaoxiong , Su Xinhao , Zhou Haoyun , Zhou Siyun , Jiang Minjie , Fang Binbo
Published: February 2024

Purpose

Patients are typically diagnosed with both hypertension and fibrosarcoma. Medical oncologists must prescribe suitable anti-hypertensive medications while considering anti-tumor drugs. Recently, immunotherapy has become prominent in cancer treatment. Nonetheless, it is unknown what role anti-hypertensive medications will play in immunotherapy.

Methods

We examined the effects of six first-line anti-hypertensive medications on programmed cell death protein 1 antibody (PD1ab) in tumor treatment using a mouse model of subcutaneous fibrosarcoma. The drugs examined were verapamil, losartan, furosemide, spironolactone, captopril, and hydrochlorothiazide (HCTZ). The infiltration of CD8+ T cells was examined by immunohistochemistry. Additionally, several in vitro and in vivo assays were used to study the effects of HCTZ on human fibrosarcoma cancer cells to explore its mechanism.

Results

Verapamil suppressed tumor growth and showed an improved effect on the tumor inhibition of PD1ab. Captopril did not affect tumor growth but brought an unexpected benefit to PD1ab treatment. In contrast, spironolactone and furosemide showed no effect on tumor growth but had an offset effect on the PD1ab therapy. Consequently, the survival time of mice was also significantly reduced. Notably, losartan and HCTZ, especially HCTZ, promoted tumor growth and weakened the effect of PD1ab treatment. Consistent results were observed in vivo and in vitro using the human fibrosarcoma cell line HT1080. We determined that the Solute Carrier Family 12 Member 3 (SLC12A3), a known target of HCTZ, may be the principal factor underlying its effect-enhancing properties through mechanism studies employing The Cancer Genome Atlas (TCGA) data and in vivo and in vitro assays.

Conclusion

Verapamil and captopril potentiated the anti-tumor effect of PD1ab, whereas spironolactone and furosemide weakened the effect of PD1ab on tumor inhibition. Alarmingly, losartan and HCTZ promoted tumor growth and impaired the effect of PD1ab. Furthermore, we preliminarily found that HCTZ may promote tumor progression through SLC12A3. Based on this study, futher mechanism researches and clinical trials should be conducted in the future.
Phenotype-specific therapeutic efficacy of ilofotase alfa in patients with sepsis-associated acute kidney injury
Authors: Bruse Niklas , Pardali Katerina , Kraan Maarten , Kox Matthijs , Pickkers Peter ,   the REVIVAL investigators
Published: February 2024

Background

There is no effective treatment for sepsis-associated acute kidney injury (SA-AKI). Ilofotase alfa (human recombinant alkaline phosphatase) has been shown to exert reno-protective properties, although it remains unclear which patients might be most likely to benefit. We aimed to identify a clinical phenotype associated with ilofotase alfa's therapeutic efficacy.

Methods

Data from 570 out of 650 patients enrolled in the REVIVAL trial were used in a stepwise machine learning approach. First, clinical variables with increasing or decreasing risk ratios for ilofotase alfa treatment across quartiles for the main secondary endpoint, Major Adverse Kidney Events up to day 90 (MAKE90), were selected. Second, linear regression analysis was used to determine the therapeutic effect size. Finally, the top-15 variables were used in different clustering analyses with consensus assessment.

Results

The optimal clustering model comprised two phenotypes. Phenotype 1 displayed relatively lower disease severity scores, and less pronounced renal and pulmonary dysfunction. Phenotype 2 exhibited higher severity scores and creatinine, with lower eGFR and bicarbonate levels. Compared with placebo treatment, ilofotase alfa significantly reduced MAKE90 events for phenotype 2 patients (54% vs. 68%, p = 0.013), but not for phenotype 1 patients (49% vs. 46%, p = 0.54).

Conclusion

We identified a clinical phenotype comprising severely ill patients with underlying kidney disease who benefitted most from ilofotase alfa treatment. This yields insight into the therapeutic potential of this novel treatment in more homogeneous patient groups and could guide patient selection in future trials, showing promise for personalized medicine in SA-AKI and other complex conditions.
Metabolic adaptation towards glycolysis supports resistance to neoadjuvant chemotherapy in early triple negative breast cancers
Authors: Derouane Françoise , Desgres Manon , Moroni Camilla , Ambroise Jérôme , Berlière Martine , Van Bockstal Mieke R. , Galant Christine , van Marcke Cédric , Vara-Messler Marianela , Hutten Stefan J. , Jonkers Jos , Mourao Larissa , Scheele Colinda L. G. J. , Duhoux Francois P. , Corbet Cyril
Published: February 2024

Background

Neoadjuvant chemotherapy (NAC) is the standard of care for patients with early-stage triple negative breast cancers (TNBC). However, more than half of TNBC patients do not achieve a pathological complete response (pCR) after NAC, and residual cancer burden (RCB) is associated with dismal long-term prognosis. Understanding the mechanisms underlying differential treatment outcomes is therefore critical to limit RCB and improve NAC efficiency.

Methods

Human TNBC cell lines and patient-derived organoids were used in combination with real-time metabolic assays to evaluate the effect of NAC (paclitaxel and epirubicin) on tumor cell metabolism, in particular glycolysis. Diagnostic biopsies (pre-NAC) from patients with early TNBC were analyzed by bulk RNA-sequencing to evaluate the predictive value of a glycolysis-related gene signature.

Results

Paclitaxel induced a consistent metabolic switch to glycolysis, correlated with a reduced mitochondrial oxidative metabolism, in TNBC cells. In pre-NAC diagnostic biopsies from TNBC patients, glycolysis was found to be upregulated in non-responders. Furthermore, glycolysis inhibition greatly improved response to NAC in TNBC organoid models.

Conclusions

Our study pinpoints a metabolic adaptation to glycolysis as a mechanism driving resistance to NAC in TNBC. Our data pave the way for the use of glycolysis-related genes as predictive biomarkers for NAC response, as well as the development of inhibitors to overcome this glycolysis-driven resistance to NAC in human TNBC patients.
Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Piétrain pigs
Authors: Ouyang Yueling , Nauwynck Hans J.
Published: February 2024

Clinically, Landrace pigs are more susceptible to porcine circovirus-associated diseases (PCVADs) than Piétrain pigs. We previously found that porcine circovirus type 2 (PCV2) can infect T-lymphoblasts. The present study examined the replication kinetics of six PCV2 strains in the lymphoblasts of Landrace and Piétrain pigs. The results showed that T-lymphoblasts from Landrace pigs are much more susceptible to PCV2 infection than those from Piétrain pigs. In addition, PCV2 replication was strain-dependent. PCV2 binding to T-lymphoblasts was partially mediated by chondroitin sulfate (CS) and dermatan sulfate (DS). Phosphacan, an effective internalization mediator in monocytes that contains several CS chains, was also demonstrated to be involved in PCV2 internalization. Viral binding and internalization were not different between the two breeds, however, the subsequent step, the disassembly was. Although inhibition of serine proteases blocked PCV2 replication in both Landrace and Piétrain pigs, this only occurred at a neutral pH in Piétrain pigs, whereas this occurred also at a low pH in Landrace. This suggested that more proteases can cleave PCV2 in Landrace lymphoblasts than in Piétrain lymphoblasts, explaining the better replication. Through co-localization studies of viral particles with endo-lysosomal markers, and quantitative analysis of organelle sizes during viral internalization, it was observed that PCV2 may exhibit a higher propensity for viral escape from late endosomes in Landrace pigs (smaller) compared to Piétrain pigs. These results provide new understandings of the different PCV2 susceptibility in Landrace and Piétrain pigs.
Confidence partitioning sampling filtering
Authors: Qiang Xingzi , Xue Rui , Zhu Yanbo
Published: February 2024

The confidence partitioning sampling filter (CPSF) method proposed in this paper is a novel approach for solving the generic nonlinear filtering problem. First, the confidence probability space (CPS) is defined, which restricts the state transition in a bounded and closed state space in the recursive Bayesian filtering. In the posterior CPS, the weighted grid samples, represented the posterior PDF, are obtained by using the partitioning sampling technique (PST). Each weighted grid sample is treated as an impulse function. The approximate expression of the posterior PDF, as key for the PST implementation, is obtained by using the properties of the impulse function in the integral operation. By executing the selection of the CPS and the PST step repeatedly, the CPSF framework is formed to achieve the approximation of the recursive Bayesian filtering. Second, the difficulty of the CPSF framework implementation lies in obtaining the real posterior CPS. Therefore, the space intersection (SI) method is suggested to obtain the approximate posterior CPS. On this basis, the SI_CPSF algorithm, as an executable algorithm, is formed to solve the generic nonlinear filtering problem. Third, the approximate error between the CPSF framework and the recursive Bayesian filter is analyzed theoretically. The consistency of the CPSF framework to the recursive Bayesian filter is proved. Finally, the performances of the SI_CPSF algorithm, including robustness, accuracy and efficiency, are evaluated using four representative simulation experiments. The simulation results showed that SI_CSPF requires far less samples than particle filter (PF) under the same accuracy. Its computation is on average one order of magnitude less than that of the PF. The robustness of the proposed algorithm is also evaluated in the simulations.
Experimental Study on Modification of Grouting Material for Joints of Prefabricated Buildings
Authors: Luo Xiaoyong , Lu Qian , Zhang Biwei
Published: February 2024

In order to apply grouting material to the joints of fabricated buildings and make it meet the performance demands of low shrinkage, strong bond, and high toughness of joint materials for prefabricated buildings, the expansion agent (EA), neoprene latex (NL), and rubber particles (RP) were used to modify the grouting material, and the effects of different dosages of the three components on the working performance, mechanical properties, and expansion or shrinkage properties of the grouting material were investigated. The results show that the EA decreases the flexural strength-to-compressive strength ratio (FCR) of the grouting material and enhances the vertical expansion rate and bond strength. The dosage of EA and the curing conditions have a significant effect on the expansion rate of the hardened grouting material. The grouting material can still maintain its 0.0022% free expansion rate with a 7% EA dosage at 120 d. The NL significantly inhibits the vertical expansion of the fresh mortar but inhibits the drying shrinkage of the grouting material after hardening, improves the FCR and bond strength; the 7 d bond strength under a 5% NL dosage can reach 4.27 MPa. The RP inhibits the vertical expansion of the fresh mortar and the drying shrinkage after mortar hardening; with the increase of its dosage, the bond strength of the grouting material increases first and then decreases, the 28 d FCS of the grouting material peaked at 0.173 at 10% dosage.
Effect of a multi-strain probiotic mixture consumption on anxiety and depression symptoms induced in adult mice by postnatal maternal separation
Authors: De Santa Francesca , Strimpakos Georgios , Marchetti Nicole , Gargari Giorgio , Torcinaro Alessio , Arioli Stefania , Mora Diego , Petrella Carla , Farioli-Vecchioli Stefano
Published: February 2024

Background

Intestinal microbial composition not only affects the health of the gut but also influences centrally mediated systems involved in mood, through the “gut-brain” axis, a bidirectional communication between gut microbiota and the brain.

In this context, the modulation of intestinal microbiota and its metabolites through the administration of probiotics seems to represent a very promising approach in the treatment of the central nervous system alterations.

Early postnatal life is a critical period during which the brain undergoes profound and essential modulations in terms of maturation and plasticity. Maternal separation (MS), i.e., the disruption of the mother–pup interaction, represents a pivotal paradigm in the study of stress-related mood disorders, by inducing persistent changes in the immune system, inflammatory processes, and emotional behavior in adult mammals.

Results

We conducted experiments to investigate whether sustained consumption of a multi-strain probiotic formulation by adult male mice could mitigate the effects of maternal separation. Our data demonstrated that the treatment with probiotics was able to totally reverse the anxiety- and depressive-like behavior; normalize the neuro-inflammatory state, by restoring the resting state of microglia; and finally induce a proneurogenic effect. Mice subjected to maternal separation showed changes in microbiota composition compared to the control group that resulted in permissive colonization by the administered multi-strain probiotic product. As a consequence, the probiotic treatment also significantly affected the production of SCFA and in particular the level of butyrate.

Conclusion

Gut microbiota and its metabolites mediate the therapeutic action of the probiotic mix on MS-induced brain dysfunctions. Our findings extend the knowledge on the use of probiotics as a therapeutic tool in the presence of alterations of the emotional sphere that significantly impact on gut microbiota composition.
A randomised controlled trial assessing the effects of weather sensitivity profile and walking in nature on the psychophysiological response to stress in individuals with coronary artery disease. A study protocol
Authors: Martinaitienė Dalia , Sampaio Francisco , Demetrovics Zsolt , Gjoneska Biljana , Portačenko Justina , Damulevičiūtė Austėja , Garbenytė-Apolinskienė Toma , Burkauskas Julius , Kažukauskienė Nijolė
Published: February 2024

Background

The following protocol pertains to a pioneer study, aiming to investigate how weather sensitivity and walking in different environments affects the psychophysiological responses to the stress of individuals with coronary artery disease (CAD) during rehabilitation (WE_SENSE_THE_NATURE). This randomised control trial will provide fresh insight on the influence of the environmental exposure in CAD patients, as it is seldom investigated in association to the disease. Additionally, findings on the link between personality traits and cognitive functions (especially cognitive flexibility), and weather sensitivity may help reveal a fine-grained perspective on the treatment possibilities for individuals with CAD at risk to stress-vulnerability.

Methods

The proposed protocol is for a randomised control trial among individuals attending a cardiac rehabilitation program. We aim to recruit 164 individuals, collecting information related to demographic characteristics, weather sensitivity, functional capacity, personality traits, subjective mental health status, cognitive function, and basal cortisol level of participating individuals. Basal cortisol level refers to cortisol concentration in saliva and will be tested in the morning and the afternoon prior to the day of the experiment. After baseline measurements, the patients will be randomly assigned to either walking outdoors or walking indoors. All measures and their sequential order will remain the same within each group, while the treatment condition (i.e., walking environment) will vary between groups. On the day of the experiment, hemodynamic parameters (assessed via 6-hour blood pressure measurements), stress level (consisting of assessments of cortisol level), and mood (assessed using visual analogues scale) will be registered. Cold stress test will be administered to evaluate the effect of walking in different environments.

Discussion

The outcomes of this study may have direct clinical applications for the use of different types of exercise environments in cardiac rehabilitation programs. Awareness about the potential influence of weather sensitivity on the psychophysiological reactions to stress in individuals with CAD may contribute to a timely planning and implementation of actions leading to improved medical care services and preventive measures, especially considering the expected weather oscillations and extreme weather events due to unfolding of the climate change.

Trial registration

This protocol has been retrospectively registered in ClinicalTrials.gov with identifier code: NCT06139705 on November 20, 2023.
Avoidance, confusion or solitude? Modelling how noise pollution affects whale migration
Authors: Johnston Stuart T. , Painter Kevin J.
Published: February 2024

Many baleen whales are renowned for their acoustic communication. Under pristine conditions, this communication can plausibly occur across hundreds of kilometres. Frequent vocalisations may allow a dispersed migrating group to maintain contact, and therefore benefit from improved navigation via the “wisdom of the crowd”. Human activities have considerably inflated ocean noise levels. Here we develop a data-driven mathematical model to investigate how ambient noise levels may inhibit whale migration. Mathematical models allow us to simultaneously simulate collective whale migration behaviour, auditory cue detection, and noise propagation. Rising ambient noise levels are hypothesised to influence navigation through three mechanisms: (i) diminished communication space; (ii) reduced ability to hear external sound cues and; (iii) triggering noise avoidance behaviour. Comparing pristine and current soundscapes, we observe navigation impairment that ranges from mild (increased journey time) to extreme (failed navigation). Notably, the three mechanisms induce qualitatively different impacts on migration behaviour. We demonstrate the model’s potential predictive power, exploring the extent to which migration may be altered under future shipping and construction scenarios.
Risk analysis for preventive conservation of heritage collections in Mediterranean museums: case study of the museum of fine arts in Alexandria (Egypt)
Authors: Elnaggar Abdelrazek , Said Mahmoud , Kraševec Ida , Said Aly , Grau-Bove Josep , Moubarak Hosam
Published: February 2024

The impacts of climate change on heritage collections in Mediterranean museums are serious and lead to accelerated material degradation, loss of value, increasing conservation costs and climatisation. Climate change scenarios and simulation models have been developed to predict the extreme and average future environmental conditions and to assess the future long-term risks caused by global warming for museum buildings and their collections in Mediterranean countries, with Egypt being particularly at risk. This paper presents the results of the risk analysis of the indoor and outdoor environments in Alexandria Museum of Fine Arts (AMFA) in Egypt to provide an overview of the current situation of conservation and museum management and to provide evidence-based data to support decision-making regarding preventive conservation given the museum's limited funding, capacity and resources. Unfortunately, the air quality in the museum cannot be considered satisfactory and specific measures need to be taken to improve the level of air quality and museum and building management. The results enabled an assessment of indoor air quality and provided information on potential risks to the museum building and collections, including variations in temperature (T) and relative humidity (RH), concentrations of NO2, SO2, O3, CO2, acetic and formic acid and lighting conditions, as well as the location and management of the museum. The results necessitate the development of a preventive conservation plan to address the challenges associated with high T/RH fluctuations and pollution pressure. This requires more regular use of the HVAC system within certain T/RH set points as well as minimising light exposure and the use of UV-filtering glazing. Care should be taken to ensure that housekeeping and emergency preparedness reduce the damping and salt florescence in the museum building. However, dealing with the impact of climate change on indoor and outdoor environments and museum collections in Mediterranean museums requires a holistic and adaptive approach that includes joint collaboration, research, training and strategic planning to ensure the long-term preservation of valuable cultural heritage collections in different climates with customised adaptations based on local environmental conditions, resources and needs. Resilience planning should be region-specific and take into account the potential impacts of extreme weather events, sea level rise and other climate-related challenges.
Comparison of deep learning-assisted blinking analysis system and Lipiview interferometer in dry eye patients: a cross-sectional study
Authors: Ren Yueping , Wen Han , Bai Furong , Huang Binge , Wang Zhenzhen , Zhang Shuwen , Pu Yaojia , Le Zhenmin , Gong Xianhui , Wang Lei , Chen Wei , Zheng Qinxiang
Published: February 2024

Background

Abnormal blinking pattern is associated with ocular surface diseases. However, blink is difficult to analyze due to the rapid movement of eyelids. Deep learning machine (DLM) has been proposed as an optional tool for blinking analysis, but its clinical practicability still needs to be proven. Therefore, the study aims to compare the DLM-assisted Keratograph 5M (K5M) as a novel method with the currently available Lipiview in the clinic and assess whether blinking parameters can be applied in the diagnosis of dry eye disease (DED).

Methods

Thirty-five DED participants and 35 normal subjects were recruited in this cross-sectional study. DED questionnaire and ocular surface signs were evaluated. Blinking parameters including number of blinks, number of incomplete blinking (IB), and IB rate were collected from the blinking videos recorded by the K5M and Lipiview. Blinking parameters were individually collected from the DLM analyzed K5M videos and Lipiview generated results. The agreement and consistency of blinking parameters were compared between the two devices. The association of blinking parameters to DED symptoms and signs were evaluated via heatmap.

Results

In total, 140 eyes of 70 participants were included in this study. Lipiview presented a higher number of IB and IB rate than those from DLM-assisted K5M (P ≤ 0.006). DLM-assisted K5M captured significant differences in number of blinks, number of IB and IB rate between DED and normal subjects (P ≤ 0.035). In all three parameters, DLM-assisted K5M also showed a better consistency in repeated measurements than Lipiview with higher intraclass correlation coefficients (number of blinks: 0.841 versus 0.665; number of IB: 0.750 versus 0.564; IB rate: 0.633 versus 0.589). More correlations between blinking parameters and DED symptoms and signs were found by DLM-assisted K5M. Moreover, the receiver operating characteristic analysis showed the number of IB from K5M exhibiting the highest area under curve of 0.773.

Conclusions

DLM-assisted K5M is a useful tool to analyze blinking videos and detect abnormal blinking patterns, especially in distinguishing DED patients from normal subjects. Large sample investigations are therefore warranted to assess its clinical utility before implementation.
Evaluating short- and long-term investment strategies: development and validation of the investment strategies scale (ISS)
Authors: Arpaci Ibrahim , Aslan Omer , Kevser Mustafa
Published: February 2024

Purpose

In response to the growing importance of understanding individual investment strategies, the present study aimed to develop a new scale for measuring both the short- and long-term investment strategies of individuals.

Design/methodology/approach

The study assessed the psychometric properties of the investment strategies scale (ISS) using data collected from 1428 individual investors. In the initial study, an exploratory factor analysis (EFA) was conducted to investigate the factor structure of the proposed scale (N = 700). The EFA results yielded a two-factor structure, and Cronbach’s alpha values for short- and long-term investment strategies were 0.90 and 0.88, respectively. A confirmatory factor analysis was performed to validate the factor structure of the scale in the second study (N = 728).

Findings

The results demonstrated that the two-factor model fit the data well. In the third study, the correlation between Hofstede’s long-term orientation and the two dimensions of the scale was investigated. The results indicated that long-term investment strategies significantly predict long-term orientation, thus confirming the concurrent validity of the scale.

Research implications

These findings demonstrate that the proposed ISS is a reliable and valid instrument for measuring individuals’ short- and long-term investment strategies, contributing to a deeper understanding of investment decision-making processes.

Originality/value

This study introduces a novel measurement tool—ISS—specifically designed to comprehensively assess both short- and long-term investment strategies among individual investors.
A step toward effective language learning: an insight into the impacts of feedback-supported tasks and peer-work activities on learners’ engagement, self-esteem, and language growth
Authors: Ramadan Elbaioumi Shaddad Ali , Jember Biruk
Published: February 2024

This study explored the impact of feedback-supported tasks and peer-work activities on language learners’ engagement, self-esteem, and language growth in a university setting in Saudi Arabia. Employing a sequential explanatory mixed-methods design, 45 participants in the experimental group and 43 in the control group underwent 15 sessions of treatment, while traditional instruction was provided to the control group. Pre- and posttests and semi-structured interviews were employed for data collection. Results indicated a significant increase in engagement and self-esteem among experimental participants compared to the control group. Language growth, assessed through a teacher-made test, also demonstrated a significant improvement in the experimental group. The findings align with Vygotsky’s Socio-Cultural Theory and Long’s Interaction Hypothesis, emphasizing the pivotal role of collaboration in language learning. However, limitations include the specific context and the absence of a delayed posttest. Implications for educators, syllabus designers, curriculum developers, and policymakers underscore the importance of integrating collaborative and feedback-oriented strategies into language instruction for enhanced learning outcomes. This research contributes valuable insights for shaping effective language education practices, while suggestions for future research encourage a more comprehensive exploration of long-term effects and contextual variations.
Anesthesia management for thoracoscopic resection of a huge intrathoracic meningocele: a case report
Authors: Nakazawa Ryosuke , Masui Kenichi , Goto Takahisa
Published: February 2024

Background

Diagnosed intrathoracic meningocele is an uncommon complication of neurofibromatosis type 1. We report an anesthesia management for a rare case undergoing thoracoscopic resection of a huge intrathoracic meningocele.

Case presentation

A 51-year-old woman was scheduled for thoracoscopic meningectomy under general anesthesia. We monitored intrathecal pressure during anesthesia to prevent a decrease in intrathecal pressure. During surgery, the intrathecal pressure occasionally increased by around 5 cmH2O immediately after the insertion of the drainage tube and occasionally decreased by up to 10 cmH2O during the careful slow aspiration of the cerebrospinal fluid (CSF). The pressure rapidly recovered after the interruption of the procedures. She was discharged on postoperative day 4 without major complications.

Conclusions

The CSF pressure was fluctuated by procedures during thoracoscopic resection of a huge meningocele. A CSF pressure monitoring was useful to detect the sudden change of CSF pressure immediately, which can cause intracranial hemorrhage.
Chelator impact: investigating the pharmacokinetic behavior of copper-64 labeled PD-L1 radioligands
Authors: Krutzek Fabian , Donat Cornelius K. , Stadlbauer Sven
Published: February 2024

Background

Programmed cell death ligand 1 (PD-L1) plays a critical role in the tumor microenvironment and overexpression in several solid cancers has been reported. This was associated with a downregulation of the local immune response, specifically of T-cells. Immune checkpoint inhibitors showed a potential to break this localized immune paralysis, but only 30% of patients are considered responders. New diagnostic approaches are therefore needed to determine patient eligibility. Small molecule radiotracers targeting PD-L1, may serve as such diagnostic tools, addressing the heterogeneous PD-L1 expression between and within tumor lesions, thus aiding in therapy decisions.

Results

Four biphenyl-based small-molecule PD-L1 ligands were synthesized using a convergent synthetic route with a linear sequence of up to eleven steps. As a chelator NODA-GA, CB-TE2A or DiAmSar was used to allow radiolabeling with copper-64 ([64Cu]Cu-14–[64Cu]Cu-16). In addition, a dimeric structure based on DiAmSar was synthesized ([64Cu]Cu-17). All four radioligands exhibited high proteolytic stability (> 95%) up to 48 h post-radiolabeling. Saturation binding yielded moderate affinities toward PD-L1, ranging from 100 to 265 nM. Real-time radioligand binding provided more promising KD values around 20 nM for [64Cu]Cu-14 and [64Cu]Cu-15. In vivo PET imaging in mice bearing both PC3 PD-L1 overexpressing and PD-L1-mock tumors was performed at 0–2, 4–5 and 24–25 h post injection (p.i.). This revealed considerably different pharmacokinetic profiles, depending on the substituted chelator. [64Cu]Cu-14, substituted with NODA-GA, showed renal clearance with low liver uptake, whereas substitution with the cross-bridged cyclam chelator CB-TE2A resulted in a primarily hepatobiliary clearance. Notably, the monomeric DiAmSar radioligand [64Cu]Cu-16 demonstrated a higher liver uptake than [64Cu]Cu-15, but was still renally cleared as evidenced by the lack of uptake in gall bladder and intestines. The dimeric structure [64Cu]Cu-17 showed extensive accumulation and trapping in the liver but was also cleared via the renal pathway. Of all tracer candidates and across all timepoints, [64Cu]Cu-17 showed the highest accumulation at 24 h p.i. in the PD-L1-overexpressing tumor of all timepoints and all radiotracers, indicating drastically increased circulation time upon dimerization of two PD-L1 binding motifs.

Conclusions

This study shows that chelator choice significantly influences the pharmacokinetic profile of biphenyl-based small molecule PD-L1 radioligands. The NODA-GA-conjugated radioligand [64Cu]Cu-14 exhibited favorable renal clearance; however, the limited uptake in tumors suggests the need for structural modifications to the binding motif for future PD-L1 radiotracers.
Predictors of blended learning adoption in higher education institutions in Oman: theory of planned behavior
Authors: Hamad Faten , Shehata Ahmed , Al Hosni Noura
Published: February 2024

The shift toward electronic learning due to the COVID-19 pandemic has created many opportunities to shape Oman’s learning styles. This study explores the factors that affect students’ acceptance of blended learning (BL) in higher education institutions in developing countries, focusing on Oman. The study examines the impact of demographic and social factors, attitude, subjective norms, perceived behavioral control, self-efficacy, beliefs, behavioral intention, and actual use of BL among students. The Theory of Planned Behavior (TPB) was used as a theoretical framework to understand the decision-making processes surrounding BL adoption. Hypotheses are formulated and tested using statistical analysis of survey results. The questionnaire was distributed to students from Sultan Qaboos University in Oman. The data collected were analyzed using inferential predictive modeling methods such as multiple regression analysis and Pearson correlation. The findings indicate that students have a positive attitude toward BL and are likely to choose it in the future. The study also reveals that demographic characteristics and various dimensions, such as attitude, subjective norms, perceived behavioral control, self-efficacy, beliefs, behavioral intention, and actual usage, influence students’ acceptance and utilization of BL. The results contribute to the existing literature and provide insights into the factors that affect BL adoption in developing countries.
A rigorous real-time acoustic positioning method for ocean bottom seismic exploration
Authors: Ma Jinye , Fang Shouchuan , Zhao Jianhu
Published: February 2024

The conventional technique for positioning seafloor geophones in ocean bottom seismic exploration encounters several challenges, including the significant impact of outliers on positioning results, underutilization of high-precision observations, and low efficiency in real-time data processing. These issues inevitably affect the quality of seismic exploration outcomes. To address these challenges and enhance the accuracy of geophone positioning, this paper proposes a rigorous real-time acoustic positioning method for geophones based on sequential adjustment and Baarda's outlier detection approach. The proposed method comprises three key steps: grouping the original acoustic observations, constructing the intra-group acoustic positioning model, and synthesizing the positioning results across the different groups. The validity and practicality of this approach are confirmed through a simulation experiment as well as the field experiment conducted in the Bohai Sea, China. The results demonstrate that the proposed method effectively eliminates outliers in the original observations and maximizes the utilization of high-quality observations. Compared to traditional acoustic positioning methods, it significantly reduces positioning errors from meters to decimeters, and in some cases can achieve centimeter-level precision. When the sound velocity profile in the operating sea area is measured, the method can attain the posterior standard deviation at the millimeter level and positioning errors within 10 cm. When the sound velocity profile is unknown, the method can achieve the posterior standard deviation at centimeter-level and positioning errors of approximately 20 cm.
Bronchogenic carcinoma: the added value of FDG PET/CT advanced volumetric and metabolic parameters in initial evaluation and their impact on prognosis and clinical outcome
Authors: Elsadawy Momena Essam Ibrahim , Omar Yehia , Taha Noha M.
Published: February 2024

Background

Bronchogenic carcinoma is considered to be one of the most common causes of cancer-related mortalities. It is divided into two main subtypes; small cell and non-small cell carcinoma. CT is considered the most commonly used radiological modality for early detection and staging. PET/CT can efficiently give both structural and functional information about the tumoral mass and malignant activity overall the body and hence can accurately assess the tumor staging and tumor response to therapy. Our study aim was to evaluate the different 18F-FDG PET/CT advanced volumetric and metabolic parameters in initial staging of bronchogenic carcinoma and their capability to predict the impact on prognostic pathway and hence the clinical outcomes.

Results

Forty patients with pathologically proven bronchogenic carcinoma were included in this study, and all of them did PET/CT in which different volumetric and metabolic parameters were measured and showed significant differences in different tumor grades.

Conclusion

PET/CT can give both structural and functional data about the tumor mass adding to its proper assessment of the initial evaluation and predicting its prognostic pathway.
Diagnostic role of serum brain-derived neurotrophic factor in HCV cirrhotic patients with minimal hepatic encephalopathy with and without schistosomiasis
Authors: Bedewy Essam S. , Elhadidi Abeer , El-Latif Naglaa Abd , Zawawy Yousra T. El , Abbasy Amany N.
Published: February 2024

Background

Liver cirrhosis (LC) advances from an asymptomatic phase (compensated cirrhosis) to a symptomatic phase (decompensated cirrhosis). Up to 80% of patients with LC may experience minimal hepatic encephalopathy (MHE), which is the first stage of hepatic encephalopathy (HE). Due to the lack of serum indicators, the diagnosis of MHE is frequently based on neuropsychometric tests. Therefore, this study aimed to evaluate the role of brain-derived neurotrophic factor (BDNF) as a diagnostic marker for MHE in HCV cirrhotic patients with or without hepatic schistosomiasis.

Patients and methods

The study consisted of 60 patients with divided into 3 groups (20 patients with HCV-related LC with overt HE, 20 patients with HCV-related LC without overt HE, and 20 patients with HCV-related LC and hepatic schistosomiases co-infection without overt HE) as well as 20 healthy controls. Patients without overt HE were evaluated for MHE by psychometric tests (trail making tests A and B). Serum BDNF was measured in all patients as well as healthy controls.

Results

Serum BDNF was found to be significantly lower in patients with LC regardless of etiology than in healthy controls; however, no statistically significant difference was found between patients with and without overt HE. Upon subdivision of patients without overt HE into “normal” and “deficient” using psychometric tests, serum BDNF was found to be significantly lower in patients with overt as well as those with “deficient” psychometric tests (have MHE). Serum BDNF had a sensitivity of 65.85% and specificity of 84.62%, and positive predictive value (PPV) was 82.0%, and negative predictive value (NPV) was 70.0% for diagnosis of MHE.

Conclusion

Serum BDNF concentration was found to be significantly lower in patients with deficient psychometric tests having either overt or covert HE which suggests that serum BDNF can be used as a diagnostic marker for MHE.
Bitcoin adoption in online payments: examining consumer intentions and word-of-mouth recommendations
Authors: Mofokeng Thabang E. , Mbeya Steven , Maduku Daniel K.
Published: February 2024

Online retailers in emerging markets like South Africa are adopting Bitcoin payments. This study explores factors driving consumer adoption and word-of-mouth (WOM) recommendations for Bitcoin in online transactions. Using an integrated model combining valency theory, social contagion theory, and the technology acceptance model (TAM), we analyse data from 521 South African online shoppers. Findings reveal that perceived usefulness, ease of use, social pressures, trust, and perceived risk significantly influence both adoption and WOM. Importantly, self-efficacy moderates the relationship between these factors and behaviour. This research contributes to the literature by offering a comprehensive understanding of Bitcoin adoption. For business and policy actors, enhancing consumer self-efficacy can foster trust, ease concerns, and encourage positive WOM, ultimately aiding successful Bitcoin implementation and promotion.
Subclinical pleuro-pulmonary disease in patients with SLE: functional and radiological methods
Authors: Hamed Hanaa S. , Abozaid Mohammed M. N. , Mekawy Enas
Published: February 2024

Background

Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with highest prevalence of chest involvement; however, early detection of subclinical pleuropulmonary diseases may improve the quality of life and prognosis of patients. This study aimed to identify the subclinical pleuro-pulmonary involvement in SLE patients without respiratory symptoms.

Methods

A total of 228 patients diagnosed with SLE were recruited and subjected to high-resolution computed tomography (HRCT) chest, ultrasound (US) chest, and spirometry for further evaluation and finding of sub-clinical signs.

Results

Around 52.63% of patients had pulmonary involvement in HRCT, while in US, it was 73.68%. Ground glass opacity was observed in 31.58% of HRCT cases, and > 1/3 of patients had pleural thickness in US. Spirometry showed that 26.32% of patients had small airway disease. SLE patients with subclinical lung involvement were significantly female and younger and had shorter disease duration, p < 0.05 for all. SLE severity showed a significant negative correlation with lung function, and was positively correlated with pleural thickness and effusion, and pleural nodules in US finding. However, diaphragmatic excursion showed a negative correlation. Moreover, ground glass opacities, honey combing opacities, interlobular septal thickening, pleural thickness, and effusion in HRCT showed positive correlation with disease severity, p < 0.001 for all, yet, the mosaic pattern showed a negative relationship.

Conclusion

The radiological assessments of SLE patients via HRCT and ultrasound unveiled prevalent findings such as ground glass opacities and pleural abnormalities. The severity of SLE correlated significantly with pulmonary function tests in a negative way, plus the positive correlation with lung opacities and pleural abnormalities.