bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of delayed access to COVID-19 vaccines in low- and lower-middle-income countries
Authors: Duroseau Brenice , Kipshidze Nodar , Limaye Rupali Jayant
Published: January 2023

Introduction

A majority of low-income (LIC) and lower-middle-income countries (LMIC) were unable to achieve at least 10% population coverage during initial vaccine rollouts, despite the rapid development of the coronavirus disease 2019 (COVID-19) vaccines. Nearly three years into this pandemic, evaluating the impact of inequities in vaccine access, uptake, and availability is long overdue. We hypothesized that a delay in receiving COVID-19 vaccines was associated with an increased toll on cumulative cases and mortality. Furthermore, this relationship was modified by the size of a country's economy.

Methods

We performed an ecological study assessing these relationships, in which a country's economic standing was assessed by world bank income classification, gross domestic product based on the purchasing power parity (GDP PPP) per capita category, and crude GDP PPP.

Results

Countries with the smallest economies reported first vaccination much later than larger economies on all three rankings, as much as 100 days longer. Among low-income countries, a one-day increase until the first vaccination was associated with a 1.92% (95% CI: 0.100, 3.87) increase in cumulative cases when compared to high-income countries (p = 0.0395) when adjusting for population size, median age, and testing data availability. Similarly, among the lowest GDP PPP countries a one-day increase until the first vaccination was associated with a 2.73% (95% CI: 0.100, 5.44) increase in cumulative cases when compared to the highest GDP PPP countries (p = 0.0415). When modeling cumulative mortality, effects in the same direction and magnitude were observed, albeit statistically non-significant.

Conclusion

Economic standing modified the effects of delayed access to COVID-19 vaccination on cumulative cases and mortality, in which LMICs tended to fare worse in outcomes than high-income countries despite the eventual rollout of vaccines. These findings highlight the importance of prioritizing equitable and timely access to COVID-19 vaccines across all countries, irrespective of economic size. Future studies should examine the impacts that vaccine inequities had on local transmission dynamics.
Movement restrictions, vaccine coverage, and reduction of the COVID-19 incidence rate in the fourth wave of the pandemic: Analysis results from 63 provinces in Vietnam
Authors: Bui Hanh My , Ha Minh Hoang , Dao Thang Phuoc , Vu Manh Duy , Pham Thai Quang , Nguyen Minh Loi , Phan Minh Hong , Nguyen Mai Thi Thanh , Hoang Xuyen Hong Thi , Ngo Huong Thu Thi , Van Minh Do , Quang Cuong Le
Published: January 2023

On April 27, 2021, the fourth wave of the coronavirus disease 2019 (COVID-19) pandemic originating from the Delta variant of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in Vietnam. The adoption of travel restrictions, coupled with rapid vaccination and mask-wearing, is a global strategy to prevent the spread of COVID-19. Although trade-off between health and economic development are unavoidable in this situation, little evidence that is specific to Vietnam in terms of movement restrictions, vaccine coverage, and real-time COVID-19 cases is available. Our research question is whether travel restrictions and vaccine coverage are related to changes in the incidence of COVID-19 in each province in Vietnam. We used Google's Global Mobility Data Source, which reports different mobility types, along with reports of vaccine coverage and COVID-19 cases retrieved from publicly and freely available datasets, for this research. Starting from the 50th case per province and incorporating a 14-day period to account for exposure and illness, we examined the association between changes in mobility (from day 27 to 04–03/11/2021) and the ratio of the number of new confirmed cases on a given day to the total number of cases in the past 14 days of indexing (the potentially contagious group in the population) per million population by making use of LOESS regression and logit regression. In two-thirds of the surveyed provinces, a reduction of up to 40% in commuting movement (to the workplace, transit stations, grocery stores, and entertainment venues) was related to a reduction in the number of cases, especially in the early stages of the pandemic. Once both movement and disease prevalence had been mitigated, further restrictions offered little additional benefit. These results indicate the importance of early and decisive actions during the pandemic.
Effects of cellulase and Lactiplantibacillus plantarum on the fermentation quality, microbial diversity, gene function prediction, and in vitro rumen fermentation parameters of Caragana korshinskii silage
Authors: Ju Ji , Zhang Guijie , Xiao Ming , Dong Chenyang , Zhang Runze , Du Liu , Zheng Yongjie , Wei Manli , Wei Manlin , Wu Baiyila
Published: January 2023

In the present study, we investigated the effects of cellulase and Lactiplantibacillus plantarum (L. plantarum) on the fermentation quality, microbial diversity, gene function prediction, and in vitro rumen fermentation parameters of the Caragana korshinskii (C. korshinskii) silage. The experimental treatment groups included control (CK), cellulase (C), L. plantarum (L), and cellulase + L. plantarum (CL). Triplicate silos were sampled after 14 and 56 days of ensiling. The microbial diversity of C. korshinskii silage at 56 days was analyzed using Illumina MiSeq sequencing technology, and the effects of C. korshinskii silage on rumen fermentation were evaluated by the in vitro artificial rumen gas production method. The results showed that the addition of cellulose and L. plantarum treatments reduced ammonia-nitrogen (NH3-N), dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber content during ensiling. Compared with the CK group, higher lactic acid content was observed in the silage of the C and L groups, but the CL group had a higher acetic acid (AA) content. Compared with the CK group, the relative abundance of Lactiplantibacillus significantly increased, but that of Enterococcus, Weissella, Enterobacter, and Pediococcus significantly decreased in all other groups except the C group (p < 0.05). The results of gene function prediction were as follows: pyruvate kinase, 1-phosphofructokinase, and fructokinase were lactate production enzymes, which suggested the association of the high AA content in the CL group to the high abundance of 6-phosphate glucose dehydrogenase. The abundance of H + -transporting ATPase and ATP-binding cassette (ABC) transporters in the L and CL groups was higher than that in the CK groups. Metabolism of terpenoids and polyketides, mismatch repair, DNA replication, nucleotide excision repair, and homologous recombination increased in the CL group compared with those in the CK group. Compared with the CK group, NH3-N and microbial protein content and the degradation rates of DM and NDF increased in the L and CL groups. To conclude, the L and CL groups had increased Lactiplantibacillus abundance, improved fermentation quality, and high DM degradation rates compared with the CK group.
Sim-to-real via latent prediction: Transferring visual non-prehensile manipulation policies
Authors: Rizzardo Carlo , Chen Fei , Caldwell Darwin
Published: January 2023

Reinforcement Learning has been shown to have a great potential for robotics. It demonstrated the capability to solve complex manipulation and locomotion tasks, even by learning end-to-end policies that operate directly on visual input, removing the need for custom perception systems. However, for practical robotics applications, its scarce sample efficiency, the need for huge amounts of resources, data, and computation time can be an insurmountable obstacle. One potential solution to this sample efficiency issue is the use of simulated environments. However, the discrepancy in visual and physical characteristics between reality and simulation, namely the sim-to-real gap, often significantly reduces the real-world performance of policies trained within a simulator. In this work we propose a sim-to-real technique that trains a Soft-Actor Critic agent together with a decoupled feature extractor and a latent-space dynamics model. The decoupled nature of the method allows to independently perform the sim-to-real transfer of feature extractor and control policy, and the presence of the dynamics model acts as a constraint on the latent representation when finetuning the feature extractor on real-world data. We show how this architecture can allow the transfer of a trained agent from simulation to reality without retraining or finetuning the control policy, but using real-world data only for adapting the feature extractor. By avoiding training the control policy in the real domain we overcome the need to apply Reinforcement Learning on real-world data, instead, we only focus on the unsupervised training of the feature extractor, considerably reducing real-world experience collection requirements. We evaluate the method on sim-to-sim and sim-to-real transfer of a policy for table-top robotic object pushing. We demonstrate how the method is capable of adapting to considerable variations in the task observations, such as changes in point-of-view, colors, and lighting, all while substantially reducing the training time with respect to policies trained directly in the real.
Governing policies and factors affecting the labor market integration of female accompanying spouses
Authors: Saurombe Musawenkosi Donia , Zinatsa Farirai
Published: January 2023

Introduction

This study sought to ascertain the macro governing policies and factors that influence the integration of female accompanying spouses in the Free State, into the South African labor market.

Methods

Utilizing a qualitative approach, thirteen one-on-one interviews, consisting of an initial purposive sample and a subsequent snowball sample, were conducted for data gathering. The study employed thematic analysis to interpret the data.

Results

The findings revealed that governing policies emerging from South Africa's migration legislation, and factors such as spouse dependence, reinforcement of traditional gender roles, and restrictive employment legislation which forced deskilling of qualifications, mainly impacted the conduct of accompanying spouses concerning the labor market.

Discussion

This study contributes to the literature on labor market integration (LMI) from an underexplored South-to-South standpoint by delving into the experiences of skilled female migrants in the family migration setting. A neglected facet of Michel Foucault's governmentality theory was used to investigate the labor market assimilation needs of female accompanying spouses. The study's qualitative approach renders the findings much less generalizable than a quantitative inquiry. It is important to note that LMI research is considerably setting-specific, despite some aspects of this study being applicable to other settings in the Global South. South Africa continues to be a pivotal regional hub for migration in the Global South, yet it has a complex migration governance framework that sets up a specific, while broadly exclusionary, macro context for accompanying spouses. This study zones in on issues that could inform more effective family migration policy.
Impact of the Productive SafetyNet Program on the minimum acceptable diet in the rural community of South Gondar Zone, Northwest Ethiopia
Authors: Engidaw Melaku Tadege , Gebremariam Alemayehu Digssie , Kefale Belayneh , Tesfa Desalegn , Tiruneh Sofonyas Abebaw , Shiferaw Yalelet Fentaw
Published: January 2023

Background

Even though numerous factors contribute to undernutrition, it can happen immediately due to poor intake and illness. A minimum acceptable diet is one of the proxy measures for adequate consumption, which is an impact indicator for the Productive SafetyNet Program (PSNP). As a result, this study aimed to assess the impact of PSNP on the minimum acceptable diet among rural infants in the South Gondar Zone, Northwest Ethiopia.

Methods

A community-based cross-sectional study design was employed in the selected woredas (districts) of South Gondar Zone, Northwest Ethiopia, from 1 December 2017 to 30 January 2017. A total of 442 participants' data were collected during this time period through interviews with their mothers or caregivers. A multistage sampling technique was employed to select study subjects and interviewees using a structured questionnaire. Stata version 16 (MP) was employed to carry out a statistical analysis. A binary logistic regression model was employed to identify significant variables. The statistical significance was declared at a p-value of more than 0.05.

Results

The highest proportion of adequate diet diversity scores (261 [59.05%]) was from households who graduated (not part of it) from PSNPs. In this study, the magnitude of adequate dietary diversity, meal frequency, and minimum acceptable diet was 79.86, 82.58, and 66.52%, respectively. Marital status (AOR = 3.98, 95% CI: 1.39, 11.40), child age (AOR = 2.82, 95% CI: 1.78, 4.47), the educational status of the father (AOR = 0.56, 95% CI: 0.35, 0.89), the wealth index (AOR = 0.27, 95% CI: 0.12, 0.64), and the place of delivery (AOR = 3.14, 95% CI: 1.47, 6.73) were significant predictors for minimum acceptable diet uptake by children.

Conclusion

In this study, two-thirds of the infants had/received a minimum acceptable diet. Furthermore, infants from households with Productive SafetyNet users had a low minimum acceptable diet. Marital status, the educational status of the father, child age, the wealth index, and the place of delivery were associated factors with having an adequate intake of minimum acceptable diet by the children. Therefore, efforts to address sociodemographic and child-related factors during routine care are crucial.
Safety and efficacy of intravesical instillation of resiniferatoxin in healthy cats: A preliminary study
Authors: Barletta Michele , Gordon Julie , Escobar André , Mitchell Krista , Trenholme H. Nicole , Grimes Janet A. , Jiménez-Andrade Juan M. , Nahama Alexis , Cisternas Alvaro
Published: January 2023

Objectives

To evaluate the safety of intravesical application of resiniferatoxin (RTX) in healthy cats and its effects on calcitonin gene-related peptide (CGRP) and substance P (SP) produced by C-fibers.

Methods

Seven adult female cats received either 25 mL of saline (control; n = 1), or intravesical RTX at 5, 25, or 50 μg in 25 mL of saline to a final concentration of 0.2 μg/mL (318 nM), 1 μg/mL (1,591 nM), and 2 μg/mL (3,181 nM) (n = 2 per group). The treatment was instilled into the urinary bladder for 20 min. Plasma concentrations of RTX were measured at 0, 0.5, 1, and 4 h. Physical exam, complete blood count, and serum biochemical analysis were performed on day 0, 7, and 14. After 14 days, the sacral dorsal root ganglia (DRG) and the urinary bladder were harvested for histological and immunofluorescence analysis.

Results

Intravesical RTX was well tolerated and plasma concentrations were below the quantifiable limits except for one cat receiving 1 μg/mL. Mild to moderate histopathological changes, including epithelial changes, edema, and blood vessel proliferation, were observed at lower doses (0.2 and 1 μg/mL), and were more severe at the higher dose (2 μg/mL). C-fiber ablation was observed in the urinary bladder tissue at all doses, as shown by an apparent reduction of both CGRP and SP immunoreactive axons.

Conclusion

A dose of 25 μg (1 μg/mL) of RTX instilled in the urinary bladder of healthy cats appeared to decrease the density of SP and CGRP nerve axons innervating bladder and induced moderate changes in the bladder tissue.
Association between the severity of histopathological lesions and Mycobacterium avium subspecies paratuberculosis (MAP) molecular diversity in cattle in southern Chile
Authors: Verdugo Cristobal , Marquez Diego , Paredes Enrique , Moroni Manuel , Navarrete-Talloni María José , Tomckowiack Camilo , Salgado Miguel
Published: January 2023

The objective was to evaluate the association between the severity of histopathological lesions caused by Mycobacterium avium subspecies paratuberculosis (MAP) infection and the molecular diversity of this pathogen. Blood, ileum, and mesenteric lymph node samples were collected at slaughter, from 1,352 adult cattle [source population 1 (SP1)]. In addition, 42 dairy herds (n = 4,963 cows) were followed for 2 years, and samples from compatible paratuberculosis clinical cases [source population 2 (SP2)] were collected. MAP infection was confirmed using an ELISA test, liquid media culture, and PCR. Isolates were genotyped using five MIRU-VNTR markers. Tissues from confirmed samples were subjected to a histopathological examination. A histopathological severity score (HSS) system was developed and used to grade (0 to 5) the magnitude of lesions caused by MAP. In general, the HSS system assesses the number of foci and degree of macrophage infiltration, together with the presence of multinucleated giant cells (MGCs) and acid-fast bacilli (AFB), in addition to the fusion of the intestinal villi and hyperplasia of the crypts. Despite the large sampling effort, only 79 MAP isolates were successfully genotyped, where 19 different haplotypes were described. A mixed-effect Poisson regression model was used to assess the relationship between haplotypes and HSS values. The model was controlled by animal age, and the farm was used as a random effect. Haplotypes were grouped based on their relative frequency: the most frequent haplotype (group i, 49.4%), the second most frequent haplotype (group ii, 12.7%), and all other haplotypes (group iii, 37.9%). Model outputs indicated that group i had significantly higher HSS values than group iii. In addition, group i was also associated with higher optical density (OD) values of the ELISA test. These results support the existence of differences in pathogenicity between MAP haplotypes. However, results were based on a relatively small sample size; thus, these should be taken with caution. Despite this, study findings suggest that haplotypes would be associated with differences in disease progression, where the dominant haplotype tends to generate more severe lesions, which could be linked to a greater shed of MAP cells than non-dominant haplotypes, increasing their chances of transmission.
The Impact of the COVID-19 Pandemic on People With Lymphedema in an Endemic Area for Lymphatic Filariasis in Brazil
Authors: Aquino Lígia Tomaz de , Aguiar-Santos Ana Maria , Rocha Abraham , Coutinho Artur Vinicius de Araujo , Nascimento Mirella Silva Batista do , Moretti Eduarda Correia , Silva Fernando Leonel da , Lima Fábia Maria , Bonfim Cristine , Medeiros Zulma
Published: January 2023

Objectives: To investigate the repercussions of the COVID-19 pandemic on lymphedema patients from an endemic area of lymphatic filariasis.

Methods: The study descriptive compared sociodemographic and clinical aspects, risk of falling and quality of life, prior and during the COVID-19 pandemic in 28 lymphedema patients, older than 18 years old and under investigation of filarial infection. For the evaluation of functional mobility, the Time Up and Go test and The Medical Outcome Study Short Form-36 Health for quality of life, was used.

Results: An increase in interdigital and dermal lesions, a higher frequency of acute dermatolymphangioadenitis crises and risk of falling, worsening of quality of life in the domains of physical functioning, general health, vitality, and mental health during the pandemic was observed.

Conclusion: Our findings of clinical worsening and quality of life of patients during the COVID-19 pandemic indicate the need to reinforce the goal of the Lymphatic Filariasis Program regarding the follow-up of these patients in the actions of the Global Program for the Elimination of Lymphatic Filariasis, due to the discontinuity in the care during the pandemic.
Impact of Digital Platform Organization on Reducing Green Production Risk to Tackle COVID-19: Evidence from Farmers in Jiangsu China
Authors: Mao Lishi , Song Junfeng , Xu Siyuan , Yu Degui
Published: January 2023

The agricultural organization based on digital platforms in C2F (Company–Platform–Farmers) may be an effective way to reduce the increased risk of green production caused by the COVID-19 pandemic, but the specific mechanism and impact involved are unclear. Applying risk cognition and decision theory, we built the theoretical framework on platform organization, pandemic risk, perception value, and green production continuity, and ascertained the impact effects and path using the PLS-SEM method. We found that the risk of COVID-19 overflow had a significant impact on farmers’ green production continuity by mediating perception value, especially regarding reduced green technology adoption intention and increased cost of living. Utilizing perfect platform value cognition, participation co-operation, access and benefit distribution, and safeguard and restraint measures of platform organization in C2F, we offered a new approach to reduce the green production risks caused by COVID-19, such as material and labor shortages, financial pressure, sales channel blockages, and price volatility. We explained the behavior-moderating motivation of farmers with different risk preferences and subjective norms in relation to tackling COVID-19. We should aim to perfect the functions of digital platform organizations to optimize the benefit linkages in C2F, accelerate farmers’ digitization ability cultivation to increase the cognitive risk level, and strengthen the policy guidance of COVID-19 prevention to reduce the influence of farmers’ subjective norms.
Optimization of Operation Parameters of the Garlic Plant Divider and Lifter Mechanisms
Authors: Zhu Zhengbo , Cheng Jianping , Wu Feng , Hu Zhichao , Yu Zhaoyang
Published: January 2023

The technology of the divider and lifter mechanisms of a garlic harvester using the depth limit, straw divide, and straw lift modes was studied in Lanling County, Shandong Province, China, according to the characteristics of high hardness, good uprightness and narrow row spacing of garlic planting. A test prototype of the divider and lifter mechanisms of the garlic harvester was designed and manufactured. Single-factor experiments and orthogonal regression experiments were carried out using the experimental factors of working speed, the angle of the divider, the height of the tip of the divider’s tooth from the ground, the ratio of the lifter’s speed to working speed and the length of the lifter’s tooth. The index was the success rate of feed. The results showed that the working speed, the angle of the divider and the length of the lifter’s tooth had a significant influence on the success rate of feed (p < 0.05), but the experimental factors of the height of the tip of the divider’s tooth from the ground and the ratio of the lifter’s speed to working speed did not have a significant influence on the success rate of feed (p > 0.05). The effects of the angle of the divider, the working speed, and the length of the divider’s tooth on the success rate of feed decreased in significance. When the working speed was 0.72 km·h−1, the length of the lifter’s tooth was 343.5 mm and the angle of the divider was 20°, the success rate of feed was the highest (98.18%). The research results are conducive to promoting high-quality and efficient combined harvesting of garlic in Lanling County, Shandong Province, China.
Development of Transgenic Maize Tolerant to Both Glyphosate and Glufosinate
Authors: Yu Xiaoxing , Sun Yongzheng , Lin Chaoyang , Wang Pengfei , Shen Zhicheng , Zhao Yu
Published: January 2023

Genetically modified (GM) crops tolerant to glyphosate have delivered significant economic benefits in farm management. However, the evolution of glyphosate resistance in weeds due to prolonged intensive use of glyphosate poses a serious threat to this weed management system. It is highly desirable in China to deploy dual herbicide-tolerant corn at the very beginning of GM corn release to delay the development of weed resistance to herbicides. Here, we report the creation and characterization of a herbicide-tolerant corn event SCB-29 that expresses both cp4 epsps and bar genes. This transgenic maize is tolerant to glyphosate up to 3600 g a.e. ha−1 and glufosinate up to 3600 g a.i. ha−1, which are quadruple the recommended rates for the two herbicides, respectively. SCB-29 is an event with only a single copy of T-DNA inserted into chromosome 10 of the maize genome. An event-specific PCR detection method was established and three generations of SCB-29 were detected by event-specific PCR suggesting that the transgenes are stably integrated into the maize genome. Analysis of the expression levels of the transgenes among plants of multiple generations by enzyme-linked immunosorbent assays suggested that the expressions are stable over different generations. Moreover, the major agronomic performances of SCB-29 appear to be similar to those of non-transgenic maize, suggesting that SCB-29 is not likely to have yield drag. Therefore, SCB-29 is an excellent herbicide-tolerant candidate to be developed into a commercial herbicide tolerance transgenic event.
From Plant Nursery to Field: Persistence of Mycorrhizal Symbiosis Balancing Effects on Growth-Defence Tradeoffs Mediated by Rootstock
Authors: Nerva Luca , Balestrini Raffaella , Chitarra Walter
Published: January 2023

The plant domestication process led to crops with strongly modified growth-defense tradeoff features, and crops that were much more pampered in terms of nutrition, irrigation and defense measures, showing less ability to trigger adaptation strategies with respect to their wild relatives. It is worth noting that plants are not alone, they share their environment with a myriad of microbes supporting them with many relevant functions. We have already demonstrated that an arbuscular mycorrhizal fungal (AMF) inoculum (formed by two AMF species, i.e., Rhizophagus irregularis and Funneliformis mosseae) is able to balance growth and defense responses in two grapevine rootstocks with opposite tradeoff features. In the present study, we evaluated the persistence of AMF-mediated balancing effects under field conditions, confirming the positive impact of the symbiosis in vineyards. In detail, some genes related to nitrogen (N) uptake and metabolism were specifically modulated by the presence of the symbionts, while others were not. Additionally, photosynthetic performances and stilbenes accumulation were influenced by the AMF presence. Overall, our results open new questions about the timing of AMF inoculation in grapevine to obtain a stable and functional symbiosis, suggesting that an early inoculation can facilitate the interaction between grapevine roots and these beneficial microorganisms.
Effects of Dietary Red Grape Extract on the Quality Traits in Juvenile European Sea Bass (Dicentrarchus labrax L.)
Authors: Tarricone Simona , Iaffaldano Nicolaia , Colonna Maria Antonietta , Giannico Francesco , Selvaggi Maria , Caputi Jambrenghi Anna , Cariglia Michela , Ragni Marco
Published: January 2023

Intensive fish farming is responsible for the occurrence of various stressors that negatively affect the health of cultured animals. Polyphenols are plant-derived compounds with biological activities and positive influences on the performances and immunity of fish. The aim of the present study was to evaluate the effects of dietary inclusion of a polyphenol extract obtained from Nero di Troia red grape on the quality of farmed sea bass (Dicentrarchus labrax) fillets. Three diets were tested: control (n = 90) received a conventional feed, whereas the two experimental groups (n = 90 each) received the control feed supplemented with the red grape polyphenol extract (GPE) at the concentration of 100 (GPE 100) or 200 mg/kg (GPE 200). The two GPE diets lowered (p < 0.05) the red (a *) and yellow (b *) indexes, fillet hardness, and total lipid content. Chewiness, concentration of saturated fatty acids, and the Atherogenic Index were higher (p < 0.05) in the control group; GPE diets increased (p < 0.05) the polyunsaturated fatty acids content. Furthermore, the concentration of malondialdehyde was lower (p < 0.05) in fillets of the GPE groups, thus confirming the antioxidant effect of the red grape extract and its effectiveness in preventing lipid oxidation.
Differentiation of Escherichia fergusonii and Escherichia coli Isolated from Patients with Inflammatory Bowel Disease/Ischemic Colitis and Their Antimicrobial Susceptibility Patterns
Authors: Dahal Ram Hari , Choi Yoon-Jung , Kim Shukho , Kim Jungmin
Published: January 2023

Genotypically, 16S rRNA gene sequence analysis clearly differentiates between species. However, species delineation between Escherichia fergusonii and Escherichia coli is much more difficult and cannot be distinguished by 16S rRNA gene sequences alone. Hence, in this study, we attempted to differentiate E. fergusonii and E. coli isolated from faecal samples of disease-associated Korean individuals with inflammatory bowel disease (IBD)/ischemic colitis (IC) and test the antimicrobial susceptibility patterns of isolated strains. Phylogenetic analysis was performed using the adenylate kinase (adk) housekeeping gene from the E. coli multi locus sequence typing (MLST) scheme. Antimicrobial susceptibility and minimum inhibitory concentration (MIC) of all disease-associated strains in addition to healthy control isolates to 14 antibiotics were determined by broth microdilution-based technique. Next, 83 isolates from 11 disease-associated faecal samples were identified as E. fergusonii using 16S rRNA gene sequence analysis. Phylogenetic analysis using the adk gene from E. coli MLST scheme revealed that most of the strains (94%) were E. coli. A total of 58 resistance patterns were obtained from 83 strains of disease-associated (IBD/IC) isolates. All isolates were resistant to at least one tested antimicrobial agent, with the highest resistance against erythromycin (88.0%), ampicillin (86.7%), ciprofloxacin (73.5%), cephalothin (72.3%), gentamicin (59%), trimethoprim-sulfamethoxazole (53%), cefotaxime (49.4%), and ceftriaxone (48.2%). A total of 90.7% of isolates were extended-spectrum beta-lactamase (ESBL)-producers among the resistant strains to third-generation cephalosporins (cefotaxime or ceftriaxone). ESBL-producing E. coli isolates from patients with Crohn’s disease (CD), ulcerative colitis (UC), and ischemic colitis (IC) were 92.3%, 82.4%, and 100%, respectively. In conclusion, adk-based phylogenetic analysis may be the most accurate method for distinguishing E. coli and E. fergusonii from Escherichia genus. We identified four loci in adk gene sequences which makes it easier to discriminate between E. coli and E. fergusonii. Additionally, we believe that gut colonization by multidrug-resistant ESBL-producing E. coli may play a significant role in IBD/IC pathogenesis.
Biofilm Formation Ability of ESBL/pAmpC-Producing Escherichia coli Isolated from the Broiler Production Pyramid
Authors: Laconi Andrea , Tolosi Roberta , Apostolakos Ilias , Piccirillo Alessandra
Published: January 2023

Escherichia coli able to produce extended spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (pAmpCs) represents a serious threat to public health, since these genes confer resistance to critically important antimicrobials (i.e., third generation cephalosporins) and can be transferred to non-resistant bacteria via plasmids. E. coli are known to be able to form a biofilm, which represents a favorable environment for the exchange of resistance determinants. Here, we assessed the ability of 102 ESBL/pAmpC-producing E. coli isolated from the broiler production pyramid to form a biofilm and to identify genetic factors involved in biofilm formation. All but one of the ESBL/pAmpC-producing E. coli were able to form a biofilm, and this represents a great concern to public health. E. coli belonging to phylogroups D, E, and F, as well as strains harboring the blaCTX-M-type gene, seem to be associated with an increased biofilm capability (p < 0.05). Furthermore, virulence genes involved in adherence and invasion (i.e., csgBAC, csgDEFG, matABCDEF, and sfaX) seem to enhance biofilm formation in E. coli. Efforts should be made to reduce the presence of ESBL/pAmpC- and biofilm-producing E. coli in the broiler production pyramid and, therefore, the risk of dissemination of resistant bacteria and genes.
Relationship between Penicillin-Binding Proteins Alterations and β-Lactams Non-Susceptibility of Diseased Pig-Isolated Streptococcus suis
Authors: Lunha Kamonwan , Chumpol Wiyada , Jiemsup Surasak , Samngamnim Sukuma , Assavacheep Pornchalit , Yongkiettrakul Suganya
Published: January 2023

Streptococcus suis is a zoonotic pathogen causing disease in both animals and humans, and the emergence of increasingly resistant bacteria to antimicrobial agents has become a significant challenge globally. The objective of this study was to investigate the genetic basis for declining susceptibility to penicillin and other β-lactams among S. suis. Antimicrobial susceptibility testing and penicillin-binding proteins (PBP1a, PBP2a, PBP2b, and PBP2x) sequence analysis were performed on 225 S. suis isolated from diseased pigs. This study found that a growing trend of isolates displayed reduced susceptibility to β-lactams including penicillin, ampicillin, amoxicillin/clavulanic acid, and cephalosporins. A total of 342 substitutions within the transpeptidase domain of four PBPs were identified, of which 18 substitutions were most statistically associated with reduced β-lactams susceptibility. Almost all the S. suis isolates which exhibited penicillin-non-susceptible phenotype (71.9%) had single nucleotide polymorphisms, leading to alterations of PBP1a (P409T) and PBP2a (T584A and H588Y). The isolates may manifest a higher level of penicillin resistance by additional mutation of M341I in the 339STMK active site motif of PBP2x. The ampicillin-non-susceptible isolates shared the mutations in PBP1a (P409T) and PBP2a (T584A and H588Y) with additional alterations of PBP2b (T625R) and PBP2x (T467S). The substitutions, including PBP1a (M587S/T), PBP2a (M433T), PBP2b (I428L), and PBP2x (Q405E/K/L), appeared to play significant roles in mediating the reduction in amoxicillin/clavulanic acid susceptibility. Among the cephalosporins, specific mutations strongly associated with the decrease in cephalosporins susceptibility were observed for ceftiofur: PBP1a (S477D/G), PBP2a (E549Q and A568S), PBP2b (T625R), and PBP2x (Q453H). It is concluded that there was genetically widespread presence of PBPs substitutions associated with reduced susceptibility to β-lactam antibiotics.
Coumarin Triazoles as Potential Antimicrobial Agents
Authors: Patil Siddappa A. , Nesaragi Aravind R. , Rodríguez-Berrios Raúl R. , Hampton Sydney M. , Bugarin Alejandro , Patil Shivaputra A.
Published: January 2023

Currently, in hospitals and community health centers, microbial infections are highly common diseases and are a leading cause of death worldwide. Antibiotics are generally used to fight microbial infections; however, because of the abuse of antibiotics, microbes have become increasingly more resistant to most of them. Therefore, medicinal chemists are constantly searching for new or improved alternatives to combat microbial infections. Coumarin triazole derivatives displayed a variety of therapeutic applications, such as antimicrobial, antioxidant, and anticancer activities. This review summarizes the advances of coumarin triazole derivatives as potential antimicrobial agents covering articles published from 2006 to 2022.
Study of the Effect of Friction Time and Preheating on the Joint Mechanical Properties of Friction Welded SS 316-Pure Zn
Authors: Dahlan Hendery , Nasution Ahmad Kafrawi , Zuhdi Sulthan Asyraf , Rusli Meifal
Published: January 2023

Friction Welding (FRW) is a solid-state welding method. This technology also permits the connecting of dissimilar and similar materials while consuming less electricity than conventional electric welding. Friction welding is frequently used to join a variety of components because it generates high-quality joints and is capable of joining a wide range of materials and their complexity. This research examined the friction welding of stainless steel and pure zinc. The investigation concentrated on the welding parameters, specifically the effect of friction time and provision of preheating on parameters with high joint strength, as well as the mechanical properties, microstructure, and characterization of the joint material. The results of the experimental research indicated that the welding settings had a significant impact on the friction welding process. The tensile strength increased as a result of the reduced friction duration during the welding process, as demonstrated by the experimental findings. The longer the friction period, the more an oxide layer will form on the surface of the metal, preventing the diffusion process and impacting the production of the intermetallic phase for the joint’s strength.
Does Silver Diamine Fluoride Affect the Adaptation of High-Viscosity Resin-Modified Glass Ionomer to Dentin? An In Vitro Study
Authors: Ghilotti James , Alzina-Cendra Arnau , Sanz José Luis , Forner Leopoldo , Llena Carmen
Published: January 2023

Background: The aim of this study was to evaluate the adaptation of a resin-modified glass ionomer to the internal walls of dentin with different conditioning systems, with or without the application of silver diamine fluoride (SDF) and potassium iodide (KI). Methods: Cervical standardized cavities were prepared on the buccal and lingual sides of 15 extracted molars. Molars were then sectioned longitudinally in a buccolingual direction, obtaining two samples from each tooth with two cavities each (60 samples). Samples were randomly divided into three groups (n = 20). Each group was divided into two subgroups. One subgroup did not receive dentin conditioning, one was conditioned with 25% polyacrylic acid—10 s (PA), and one with 37% orthophosphoric acid—5 s (OPA). In the other subgroup, dentine was treated with SDF/KI and not conditioned or conditioned with PA or OPA. All cavities were filled with Riva Light Cure® (RLC). The adaptation of the RLC to the cavity walls was evaluated by SEM at 100× magnification. The area of maximum interfacial gap was magnified at 1000× to measure its size. Kruskal–Wallis and Mann–Whitney U test were used for comparison. A significance level of α = 0.05 was used. Results: No significant differences in the percentage of well-adapted samples were found in subgroups when SDF/KI was used, regardless of whether conditioner was used or not and whether PA or OPA was used (p > 0.05). Regardless of the gap size, dentin treatment with SDF/KI did not negatively influence ionomer adaptation to dentin walls significantly (p > 0.05), except for the subgroup conditioned with OPA (p < 0.05). Furthermore, it improved the adaptation in the axial wall of the subgroup without dentin conditioning (p < 0.05). Conclusion: According to the results of the present in vitro study, the use of SDF/KI did not affect RLC adaptation to the cavity walls. Subsequent use of a conditioner worsens the adaptation of the material to the cavity walls.
Design of a Saving-Energy Fuzzy Logic Controller for a Differential Drive Robot Based on an Optimization
Authors: Pham Van Thuan , Stefek Alexandr , Krivanek Vaclav , Nguyen Thi Son
Published: January 2023

In the problem of designing a Fuzzy Logic Controller (FLC) for robots in general and a Differential Drive Robot (DDR) in particular, the determination of the parameters of Membership Functions (MFs) and Fuzzy Rules (FRs) is a difficult, complicated, and time-consuming problem because this is mainly based on heuristics and the knowledge of experts. Therefore, this paper provides a new method to efficiently design an FLC for the DDR by using the Genetic Algorithm (GA). Here, the GA is used to generate and optimize both the parameters of MFs and the FRs according to the minimum kinetic energy loss criterion. For this purpose, a program is created in Google Colab® by using the Python language with the help of the “Pymoo” library to not only automatically generate all the suboptimal parameters of MFs and the suboptimal FRs but also the simulate and evaluate different used FLCs. This program is published as an open-source code so that all readers can browse, view, run, and modify the code themselves to design their FLC. The simulation results have shown that the designed FLC is much better than other used FLCs in terms of the minimum kinetic energy loss while other control performances are still good.
Performability Evaluation and Sensitivity Analysis of a Video Streaming on Demand Architecture
Authors: Sousa Rubenilson de , Cristian Leonardo , Feitosa Leonel , Choi Eunmi , Nguyen Tuan Anh , Min Dugki , Silva Francisco Airton
Published: January 2023

In urban air mobility (UAM), video streaming platforms have gained significant attention from media companies due to their growing necessity for on-demand video streaming services-as-you-go in flights. Video streaming services can provide constant data transactions in a huge amount, especially in its operational digital twin (ODT). As a result, the ability to provide a satisfactory user experience through video streaming platforms is critical and complex. This requires continuously operating services while handling numerous user requests for near real-time video streaming. At the same time, high-quality video with high resolution and minimal interruptions is often expected. Therefore, the availability and performance (i.e., performability) of the Back-End video streaming infrastructure are crucial parameters for these platforms. However, evaluating novel video-on-demand architectures in real-world scenarios can be costly due to the numerous parameters involved. Analytical models, such as stochastic Petri nets (SPNs), can serve as an alternative in this complex scenario as they can be used to analyze systems during the design process. In this study, we developed a set of SPN models to assess the performance of a video-on-demand system. These models were designed to illustrate and to evaluate a video-on-demand architecture while considering performance. We had a base SPN model as well as three enhanced variants available. The extended models were generated using the Design of Experiments (DoE) technique and sensitivity analysis results. The DoE identified the factors with the greatest impacts on performance, and the most significant factor interactions. Redundancy strategies were applied to the extended models to increase the availability of the most important components. This redundancy increased the availability of “9 s” from three to five. It is worth noting that this study can help the designers of video streaming systems, to plan and to optimize their ideas based on the provided models.
A Structured Methodology to Simulate Composite Advanced Joint Behavior for Ultra-Light Platforms Applications
Authors: Polla Alessandro , Frulla Giacomo , Cestino Enrico , Das Raj , Marzocca Pier
Published: January 2023

Numerical simulations have the potential to be used for designing damage-tolerance composite structures. However, numerical models are currently computationally intensive, and their post-failure evolution and fracture morphology predictions are still limited. In the present work, a numerical methodology to simulate advanced composite joints is presented. The results of a numerical campaign aimed to evaluate the progressive damage and failure analysis (PDFA) of an advanced pin-hole connection under tensile and compressive load are evaluated. A high-fidelity stacked shell-cohesive methodology is employed to simulate the ultimate load, fracture initiation, and propagation of the proposed composite joint. Post-failure erosion methodology is proposed to control the initiation and evolution of composite fractures. The location and extension of the numerically predicted damages are compared with experimental observations. The proposed methodology demonstrates its preliminary ability to be used for designing composite joints up to failure. Specific outcomes are also pointed out.
Classification and Provenance on Geochemical Lithogenes: A Case Study on Rock–Soil–Sediment System in Wanquan Area of Zhangjiakou, North China
Authors: An Yonglong , Yin Xiulan , Gong Qingjie , Li Xiaolei , Liu Ningqiang
Published: January 2023

Geochemical lithogenes have been successfully applied as an innovative concept in the field of composition classification and source traceability of geological materials recently. This paper introduces the background of the development of geochemical genes and the construction and application of LG01 and LG03 lithogenes. Based on LG01 and LG03, the LG_CR classification and provenance are applied and verified on a weathering profile, ten gully sedimentary profiles and regional stream sediments in the Wanquan area of Zhangjiakou city, Hebei province, China. The geochemical lithology of the weathering profile shows a gradual variation from basic-like in the bottom rock to acidic-like at the upper soils compositionally with heterogeneity. The classification results on 10 sedimentary gully profiles (each with five samples) indicate that soils at the bottom of the gully system are dominated with 11 types of LG_CR materials, while the top materials are made up of 21 types, reflecting the mixing of the upstream soils. The results of stream sediments from a regional geochemical survey with a scale of 1:200,000 in this area illustrate that the classification results of LG_CR on stream sediments are basically consistent with the petrological results derived from regional geological mapping. Therefore, LG_CR can be used not only as an effective tool for classification and traceability of geological materials but also has great potential in lithological mapping in petrological-overburdened areas.
Pix2Pix and Deep Neural Network-Based Deep Learning Technology for Predicting Vortical Flow Fields and Aerodynamic Performance of Airfoils
Authors: Song Han-Seop , Mugabi Jophous , Jeong Jae-Ho
Published: January 2023

Traditional computational fluid dynamics (CFD) methods are usually used to obtain information about the flow field over an airfoil by solving the Navier–Stokes equations for the mesh with boundary conditions. These methods are usually costly and time-consuming. In this study, the pix2pix method, which utilizes conditional generative adversarial networks (cGANs) for image-to-image translation, and a deep neural network (DNN) method were used to predict the airfoil flow field and aerodynamic performance for a wind turbine blade with various shapes, Reynolds numbers, and angles of attack. Pix2pix is a universal solution to the image-to-image translation problem that utilizes cGANs. It was successfully implemented to predict the airfoil flow field using fully implicit high-resolution scheme-based compressible CFD codes with genetic algorithms. The results showed that the vortical flow fields of the thick airfoils could be predicted well using the pix2pix method as a result of deep learning.