bluuebunny/crossref_metadata_2025 huggingface dataset:
Alternative Effects Yeast-Based Biostimulants Against Downy Mildew in Vitis vinifera cv Cabernet Sauvignon
Authors: Puccioni Sergio , Biselli Chiara , Perria Rita , Zanella Gianmaria , D’Arcangelo Mauro Eugenio Maria
Published: February 2025

Grapevine downy mildew, caused by Plasmopara viticola, is one of the most destructive vineyard diseases worldwide, with the potential to devastate up to 90% of harvests under adverse conditions. Traditional chemical-based control strategies are increasingly restricted due to environmental concerns and the development of resistant pathogen strains, prompting the search for sustainable alternatives. This study evaluated the efficacy of two yeast-based biostimulants (YE1, an experimental formulation, and YE2, Romeo, a commercial preparation containing Cerevisane®) in mitigating P. viticola infections in Vitis vinifera cv Cabernet Sauvignon under open field conditions. Field trials were designed to monitor the effects of these bioproducts on grapevine metabolism, with a focus on the biosynthesis of phenolic compounds linked to plant defense responses. Both biostimulants demonstrated significant efficacy against downy mildew, achieving protection levels above 93% during critical growth stages. Metabolic analyses of leaves revealed distinct impacts of YE1 and YE2 on secondary metabolite dynamics. YE1 primarily promoted the accumulation of flavonols, such as quercetin glycosides and kaempferol glucosides, which are known to contribute to pathogen resistance through antimicrobial activity and ROS detoxification. Compared to the untreated sample, quercetin glycosides levels in YE1 leaves were approximately 700 µg/g higher (+9.6%), in comparison to control conditions, on a dry weight basis after the first treatment in 2019 and about 900 µg/g higher (+13.7%) after the final treatment in 2020. A similar trend was observed for kaempferol concentrations, which were 70 µg/g (+98.7%) and 100 µg/g higher (+24.6%) on the same dates. In contrast, YE2 enhanced the biosynthesis of caftaric and coutaric acids, known for their antioxidant and antimicrobial properties. These compounds were induced by YE2 over time and, after the last treatment, in 2019 caftaric acid levels were approximately 345 µg/g higher (+25.9%), than control condition, while coutaric acid levels were 30 µg/g higher (+33.8%). The stilbene piceid was also induced at early stages by treatments, indicating its role in enhancing plant defenses. In 2019, after the first treatment, both YE1 and YE2 applications induced an increase in piceid concentration, compared to the control, of approximately 2.5 µg/g (+104.8%) for YE1 and 1.3 µg/g for Y2 (+55.0%). In contrast, in 2020, the effect was more pronounced for YE2, with concentrations exceeding the control by 2 µg/g (+46.2%). The results suggest that these yeast-based bioproducts act as elicitors, effectively enhancing grapevine immunity and reducing reliance on synthetic inputs. This study provides novel insights into the metabolic mechanisms underlying the efficacy of yeast-based biostimulants against downy mildew and offers practical guidance for their strategic application.
Possible Racial Disparities in the Diagnosis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)
Authors: Jones Chloe Lisette , Younger Jarred
Published: February 2025

Myalgic encephalomyelitis (ME/CFS) a chronic, disabling illness with no established etiopathology. It has been indicated in some population-based studies that Black and ethnic minority populations are underdiagnosed with ME/CFS. The aims of the present study were to (1) identify the agreement between receiving an ME/CFS diagnosis and meeting diagnostic criteria, (2) identify the demographic characteristics associated with receiving a diagnosis, and (3) explore patient satisfaction with healthcare. Self-reported medical history and symptoms were collected via online survey from respondents with and without fatigue. The agreement between self-reporting an ME/CFS diagnosis and meeting the Center for Disease Control’s (CDC) ME/CFS criteria or Institute of Medicine (IOM) criteria was assessed with Cohen’s kappa. Patient characteristics predicting a physician diagnosis were analyzed with logistic regression. Associations between diagnosis, demographics, and healthcare satisfaction were assessed with chi-square tests of independence. There were 1110 responses. The agreement between meeting ME/CFS criteria and reporting an ME/CFS diagnosis was fair (CDC: κ = 0.29; SE = 0.02; IOM: κ = 0.28, SE = 0.03). White respondents had 2.94 greater odds of being diagnosed with ME/CFS than non-White respondents. Having an ME/CFS diagnosis was associated with dissatisfaction with healthcare (χ2 (3, N = 1063) = 14.17, p = 0.003). The findings suggest racial disparities in the diagnostic processes for ME/CFS.
Correction: Oliveira et al. Discovery of a Necroptosis Inhibitor Improving Dopaminergic Neuronal Loss after MPTP Exposure in Mice. Int. J. Mol. Sci. 2021, 22, 5289
Authors: Oliveira Sara R. , Dionísio Pedro A. , Gaspar Maria M. , Ferreira Maria B. T. , Rodrigues Catarina A. B. , Pereira Rita G. , Estevão Mónica S. , Perry Maria J. , Moreira Rui , Afonso Carlos A. M. , Amaral Joana D. , Rodrigues Cecília M. P.
Published: February 2025

In the original publication [...]
α-Synuclein Degradation in Brain Pericytes Is Mediated via Akt, ERK, and p38 MAPK Signaling Pathways
Authors: Yokoya Miki , Takata Fuyuko , Iwao Takuro , Matsumoto Junichi , Tanaka Yasuyoshi , Aridome Hisataka , Yasunaga Miho , Mizoguchi Junko , Sano Kazunori , Dohgu Shinya
Published: February 2025

Parkinson’s disease (PD) is characterized by widespread distribution of Lewy bodies, which are composed of phosphorylated and aggregated forms of α-Synuclein (α-Syn), in the brain. Although the accumulation and propagation of α-Syn contribute to the development of PD, the involvement of the blood–brain barrier (BBB) in these processes remains unknown. Pericytes, one of the cell types that constitute the BBB, degrade various forms of α-Syn. However, the detailed mechanisms involved in α-Syn degradation by pericytes remain poorly understood. Therefore, in this study, we aimed to determine the ability of the BBB-constituting cells, particularly primary cultures of rat pericytes, brain endothelial cells, and astrocytes, to degrade α-Syn. After α-Syn uptake by the cells, intracellular α-Syn decreased only in pericytes. This pericyte-specific α-Syn decrease was inhibited by an autophagy inhibitor, bafilomycin A1, and a proteasome inhibitor, MG132. siRNA-mediated knockdown of degradation enzymes or familial PD-associated genes, including cathepsin D, DJ-1, and LRRK2, did not affect α-Syn clearance in pericytes. However, pharmacological inhibitors of Akt, ERK, and p38 MAPK inhibited α-Syn degradation by pericytes. In conclusion, our results suggest that α-Syn degradation by pericytes is mediated by an autophagy–lysosome system and a ubiquitin–proteasome system via α-Syn-activated Akt, ERK, and p38 MAPK signaling pathways.
Pro-Tumorigenic Effect of Continuous Cromolyn Treatment in Bladder Cancer
Authors: Franković Lucija , Degoricija Marina , Gabela Ivana , Vilović Katarina , Korac-Prlic Jelena
Published: February 2025

Globally, bladder cancer is the sixth most frequently diagnosed cancer among men. Despite the increasing availability of immunomodulatory treatments for bladder cancer, the survival rates are still low, which calls for potential new drug-repurposing targets. This study aimed to investigate the effects of cromolyn, a mast cell (MC) stabilizer in allergic reactions, on a subcutaneous tumor model with a syngeneic mouse MB49 bladder cancer cell line. A concentration of 50 mg/kg of cromolyn was daily administered intraperitoneally in a 4-day therapeutic protocol to mice with established tumors and in a continuous 11-day protocol which started one day prior to the subcutaneous injection of tumor cells. Therapeutic treatment demonstrated a marked downregulation of genes related to angiogenesis and upregulation of genes related to cytotoxic T-cell and NK cell activity. Conversely, continuous cromolyn treatment suppressed genes involved in immune cell recruitment and activation, as well as apoptotic and necroptotic pathways, leading to a greater tumor burden (+142.4 mg [95CI + 28.42, +256.4], p = 0.0158). The same pro-tumorigenic effect was found in mast cell-deficient mice (KitW-sh/W-sh + 301.7 mg [95CI + 87.99, 515.4], p = 0.0079; Cpa3Cre/+ +107.2 mg [95CI − 39.37, +253.57], p = 0.1423), indicating that continuous cromolyn treatment mostly acts through the inhibition of mast cell degranulation. In summary, our results demonstrate the distinct effects of cromolyn on tumor progression, which depend on the protocol of cromolyn administration.
ADCY5 Gene Affects Seasonal Reproduction in Dairy Goats by Regulating Ovarian Granulosa Cells Steroid Hormone Synthesis
Authors: Shi Chenbo , Zhang Fuhong , He Qiuya , Man Jianjun , Mu Yuanpan , Zhao Jianqing , Zhu Lu , Loor Juan J. , Luo Jun
Published: February 2025

Follicle development in dairy goats is lower after induced estrus during the non-breeding season, reducing conception rates and challenging year-round milk supply. This study investigated follicle development during the breeding and non-breeding seasons and explored molecular mechanisms for variations in the proportions of follicles of different sizes using ovarian RNA-seq and in vitro experiments. Induced estrus during the non-breeding season used a simulated breeding season short photoperiod and male effect methods, while the male effect method was used during the breeding season. This study identified an increase in follicle size during the breeding season and performed RNA-seq on ovaries to explore the underlying causes. The RNA-seq analysis elucidated pathways associated with cellular and hormonal metabolism and identified adenylyl cyclase 5 (ADCY5) as a key differentially expressed gene. In vitro experiments demonstrated that interfering with ADCY5 in ovarian granulosa cells (GCs) reduced steroid synthesis. Conversely, the overexpression of ADCY5 increased steroid synthesis. ADCY5 affects the biological function of GCs and consequently influences follicle development through the cAMP-response element binding protein (CREB) and p38 mitogen-activated protein kinase phosphorylation (MAPK) pathways. Overall, our findings demonstrate that follicle development in dairy goats differs between the breeding and non-breeding seasons and that the differential expression levels of the ADCY5 gene contribute to this discrepancy.
Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery
Authors: Puchta-Jasińska Marta , Bolc Paulina , Pietrusińska-Radzio Aleksandra , Motor Adrian , Boczkowska Maja
Published: February 2025

Small interfering RNAs (siRNAs) are a distinct class of regulatory RNAs in plants and animals. Gene silencing by small interfering RNAs is one of the fundamental mechanisms for regulating gene expression. siRNAs are critical regulators during developmental processes. siRNAs have similar structures and functions to small RNAs but are derived from double-stranded RNA and may be involved in directing DNA methylation of target sequences. siRNAs are a less well-studied class than the miRNA group, and researchers continue to identify new classes of siRNAs that appear at specific developmental stages and in particular tissues, revealing a more complex mode of siRNA action than previously thought. This review characterizes the siRNA classes and their biogenesis process and focuses on presenting their known functions in the regulation of plant development and responses to biotic and abiotic stresses. The review also highlights the exciting potential for future research in this field, proposing methods for detecting plant siRNAs and a bioinformatic pathway for identifying siRNAs and their functions.
Copper Nanoparticles Synthesized by Chemical Reduction with Medical Applications
Authors: Pricop Alexandra , Negrea Adina , Pascu Bogdan , Nemeş Nicoleta Sorina , Ciopec Mihaela , Negrea Petru , Ianăşi Cătălin , Svera Paula , Muntean Delia , Ivan Alexandra , Cristea Iustina Mirabela
Published: February 2025

Copper nanoparticles (CuNPs) have attracted attention due to their low cost and high specific surface area. In this work, a simple and inexpensive two-step synthesis method was proposed to prepare highly stable and well-dispersed spherical CuNPs in solution with a particle size of approximately 37 nm. Synthesis of CuNPs was carried on in the presence of complexing agent trisodium citrate (TSC), while for the chemical reduction step, sodium borohydride (NaBH4) was used. Taking into account the potential of this type of nanoparticles, their synthesis and characterization represent a current and relevant topic in the field. The ability to control the size, shape and properties of CuNPs by adjusting the synthesis parameters (pH, precursor:stabilizer:reductant ratio, homogenization time, temperature) offers extraordinary flexibility in the development of these materials. The combination of characterization techniques such as SEM, EDX, UV–Vis, Raman, FT-IR and AFM provides a thorough understanding of the structure and properties of CuNPs, allowing the modulation of the properties of the obtained nanoparticles in the desired direction. Based on the studies, the copper reduction mechanism was proposed. For the theoretical verification of the size of the experimentally obtained spherical CuNPs, Mie theory was applied. A stability study of the synthesized CuNPs in optimal conditions was performed using UV–Vis analysis at specific time intervals (1, 3, 30 and 60 days), the sample being kept in the dark, inside a drawer at 25 °C. The CuNPs obtained after setting the optimal synthesis parameters (Cu(II):TSC:BH4+ = 1:1:0.2, pH = 5, homogenization time 60 min and temperature 25 °C) were then tested to highlight their antibacterial effect on some reference bacterial strains. The obtained CuNPs demonstrated very good antimicrobial efficacy compared to traditional antimicrobials, for both Gram-negative and Gram-positive bacteria. This may reduce the development of antimicrobial resistance, an urgent medical issue. After evaluating the cytotoxic effects of CuNPs on the SKBR3 cancer cell line, a significant decrease in cell proliferation was observed at the 0.5 mg/mL concentration, with a reduction of 89% after 60 h of cultivation. Higher concentrations of CuNPs induced a more rapid cytotoxic effect, leading to an accelerated decline in cell viability.
Serotonin Mitigates ColdStress-Induced Damage in Kandelia obovata Through Modulating the Endogenous Melatonin- and Abscisic Acid Biosynthesis
Authors: Shan Qiaobo , Liu Weicheng , Ni Xiaoxiao , Li Min , Sun Yifan , Liao Lixian , Zheng Chunfang
Published: February 2025

Endogenous melatonin (MEL) and abscisic acid (ABA) are involved in the adaptation of plants to environmental stresses. The application of exogenous serotonin (SER) to plants can enhance their tolerance to abiotic stress, such as cold. However, the mechanism associated with serotonin-mediated defense against cold-induced damage in mangroves is still poorly understood. In this study, we demonstrated that mangrove (Kandelia obovata) seedlings sprayed with 200 μmol·L−1 serotonin exhibited enhanced cold tolerance, as shown by reduced damage to leaves and loss of photosynthesis when exposed to low-temperature conditions. The mechanism associated with the cold adaptation of K. obovata seedlings upon treatment with serotonin was subsequently investigated by transcriptomic analysis. Serotonin treatment caused changes in differentially expressed genes (DEGs) involved in the regulation of melatonin (MEL) and ABA biosynthesis and defense responses against cold stress. Under low-temperature stress, serotonin-treated seedlings showed a significant increase in the endogenous levels of melatonin and ABA. By contrast, under normal growth conditions, K. obovata seedlings treated with serotonin displayed no substantial change in melatonin level, whereas ABA level significantly increased. These findings demonstrated that serotonin treatment might play an important role in the enhanced resistance to cold in K. obovata and that such an effect would depend on the activation of endogenous melatonin and ABA synthesis.
Anthelmintic Potential and In Silico Studies of Ricinoleic Acid from the Seed Oil of Ricinus communis L.
Authors: Berhanu Temesgen , Tewelde Eyael , Yeshak Mariamawit Y. , Bisrat Daniel , Asres Kaleab
Published: February 2025

The prevalence of human intestinal helminth parasitic infections is extensive, with over half of the global population estimated to suffer from these infections. Traditionally, various plant species, including Ricinus communis L. (Euphorbiaceae), are used to treat helminth infections. In this study, ricinoleic acid was isolated from the base hydrolysate of the petroleum ether seed extract of R. communis using column chromatography and transformed into ricinoleic acid methyl ester through esterification. The extract, ricinoleic acid and its methyl ester were evaluated for their anthelmintic activities against the model organism Caenorhabditis elegans. The results revealed that at a concentration of 1 mg/mL, ricinoleic acid and its methyl ester killed 97.40% and 97.83% of C. elegans worms, respectively. Molecular docking studies of ricinoleic acid on succinate dehydrogenase (SDH), glucose-6-phosphate 1-dehydrogenase (G6PD), and tubulin beta-2 chain (TBB2C) revealed that ricinoleic acid has a more favorable interaction with succinate dehydrogenase (−5.408 kcal/mol) compared to glucose-6-phosphate 1-dehydrogenase (−3.758 kcal/mol) and tubulin beta-2 chain (−1.444 kcal/mol). Furthermore, Absorption, Distribution, Metabolism, and Excretion (ADME) analyses unveiled that ricinoleic acid adheres to Lipinski’s rule of five, positioning it as a potential compound to treat helminths. The current study demonstrated that R. communis seed oil possesses genuine anthelmintic activity against C. elegans, which is likely due to ricinoleic acid.
Shrimp Shapes a Nitrite Tolerance Trait via Regulating Autophagy and Apoptosis
Authors: Zhou Liping , Ma Zhentao , Liu Qingyun , Li Qiangyong , Peng Min , Yang Chunling , Zhang Bin , Chen Tiancong , Huang Yuliu , Zheng Zhihong , Huang Aiguo , Chen Xiuli , Zhang Yueling , Zhao Xianliang , Zhao Yongzhen
Published: February 2025

Nitrite is a significant toxic substance that causes acute toxicity in aquatic animals. However, the molecular mechanisms underlying nitrite tolerance remain largely unidentified. Here, we investigated the physiological and transcriptomic profiles of nitrite-sensitive and nitrite-tolerant families of the white shrimp Penaeus vannamei under nitrite stress. Exposure to a lethal concentration of nitrite for 72 h caused higher mortality, higher nitrite accumulation, and more severe physiological damage in both the hemolymph and hepatopancreas of nitrite-sensitive families than in nitrite-tolerant families. This damage manifested as hepatic tubular deformation, hepatocyte necrosis, decreased hemocyte counts, lowered phagocytic activity in hemocytes, elevated production of reactive oxygen species (ROS), and decreased AKP enzyme activity. Furthermore, transcriptomic analysis revealed an upregulation in protein processing in the endoplasmic reticulum (ER) in the nitrite-tolerant families, triggering apoptosis- and autophagy-related pathways in the hemocytes and hepatopancreas upon nitrite exposure. Additionally, TUNEL staining and transmission electron microscopy (TEM) experiments revealed that the tolerant families may mitigate nitrite toxicity by modulating autophagy and apoptosis. Collectively, our results provide a valuable foundation for exploring the molecular mechanisms underlying nitrite tolerance in shrimp, which could facilitate the targeted breeding of shrimp germplasm with enhanced nitrite tolerance in aquaculture.
HMGA2 Overexpression in Papillary Thyroid Cancer Promotes Thyroid Cell Dedifferentiation and Invasion, and These Effects Are Counteracted by Suramin
Authors: Van Branteghem Cindy , Henry Nicolas , Craciun Ligia , Maenhaut Carine
Published: February 2025

Thyroid cancer is the most prevalent endocrine malignancy, and papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy. While PTC generally has a favorable prognosis, a subset dedifferentiates into aggressive forms. However, the molecular mechanisms responsible for aggressiveness and dedifferentiation are still poorly understood. We previously showed that HMGA2, a non-histone architectural transcription factor overexpressed in PTC, is involved in cell invasion. This study aimed to further analyze the role of HMGA2 in PTC tumorigenesis by exploring the expression of thyroid-specific and EMT-related genes following HMGA2 knockdown in thyroid cancer cell lines. Then, the clinical relevance of our data was evaluated in vivo. HMGA2 silencing did not modulate the expression of EMT related genes but led to the increased expression of thyroid differentiation genes. Our data also suggest that the MAPK pathway induces thyroid cell dedifferentiation through HMGA2. On the other hand, forskolin, promoting thyroid differentiation, decreased HMGA2 expression. The negative correlations between HMGA2 and thyroid-specific gene expressions were confirmed in a transgenic mouse model of PTC and in human PTC. Finally, we showed that HMGA2 inhibition by suramin reduced cell invasion and induced differentiation expression in vitro, indicating a new therapeutic strategy for treating thyroid cancer.
Comprehensive Bioinformatics Analysis of Glycosylation-Related Genes and Potential Therapeutic Targets in Colorectal Cancer
Authors: Chuang Po-Kai , Chang Kai-Fu , Chang Chih-Hsuan , Chen Ting-Yu , Wu Yueh-Jung , Lin Hui-Ru , Wu Chi-Jen , Wu Cheng-Chun , Ho Yu-Cheng , Lin Chih-Chun , Yuan Chien-Han , Wang Chih-Yang , Lee Yung-Kuo , Chen Tung-Yuan
Published: February 2025

Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide, characterized by high incidence and poor survival rates. Glycosylation, a fundamental post-translational modification, influences protein stability, signaling, and tumor progression, with aberrations implicated in immune evasion and metastasis. This study investigates the role of glycosylation-related genes (Glycosylation-RGs) in CRC using machine learning and bioinformatics. Data from The Cancer Genome Atlas (TCGA) and the Molecular Signatures Database (MSigDB) were analyzed to identify 67 differentially expressed Glycosylation-RGs. These genes were used to classify CRC patients into two subgroups with distinct survival outcomes, highlighting their prognostic value. Weighted gene coexpression network analysis (WGCNA) revealed key modules associated with CRC traits, including pathways like glycan biosynthesis and PI3K–Akt signaling. A machine-learning-based prognostic model demonstrated strong predictive performance, stratifying patients into high- and low-risk groups with significant survival differences. Additionally, the model revealed correlations between risk scores and immune cell infiltration, providing insights into the tumor immune microenvironment. Drug sensitivity analysis identified potential therapeutic agents, including Trametinib, SCH772984, and Oxaliplatin, showing differential efficacy between risk groups. These findings enhance our understanding of glycosylation in CRC, identifying it as a critical factor in disease progression and a promising target for future therapeutic strategies.
Genome-Wide Identification and Expression Analyses of Glycoside Hydrolase Family 18 Genes During Nodule Symbiosis in Glycine max
Authors: Li Rujie , Gou Chuanjie , Zhang Ke , He Milan , Li Lanxin , Kong Fanjiang , Sun Zhihui , Liu Huan
Published: February 2025

Glycoside hydrolase family 18 (GH18) proteins can hydrolyze the β-1,4-glycosidic bonds of chitin, which is a common structure component of insect exoskeletons and fungal cell walls. In this study, 36 GH18 genes were identified and subjected to bioinformatic analysis based on the genomic data of Glycine max. They were distributed in 16 out of 20 tested soybean chromosomes. According to the amino acid sequences, they can be further divided into five subclades. Class III chitinases (22 members) and class V chitinases (6 members) are the major two subclades. The amino acid size of soybean GH18 proteins ranges from 173 amino acids (aa) to 820 aa and the molecular weight ranges from 19.46 kDa to 91.01 kDa. From an evolutionary perspective, soybean GH18 genes are closely related to Medicago (17 collinear loci with soybean) and Lotus (23 collinear loci with soybean). Promoter analysis revealed that GH18 genes could be induced by environmental stress, hormones, and embryo development. GmGH18-15, GmGH18-24, and GmGH18-33 were screened out due to their nodulation specific expression and further verified by RT-qPCR. These results provide an elaborate reference for the further characterization of specific GH18 genes, especially during nodule formation in soybean.
Genetically Elevated Selenoprotein S Levels and Risk of Stroke: A Two-Sample Mendelian Randomization Analysis
Authors: He Yan , Liu Yi , Meng Haoliang , Sun Jinsheng , Rui Yukun , Tian Xiaoyi , Zhu Zhengbao , Gao Yuzhen
Published: February 2025

Selenoprotein S (SELENOS), one of the carrier proteins of dietary selenium (Se), is a key regulator of inflammation, oxidative stress, and endoplasmic reticulum (ER) stress, all of which are implicated in the pathogenesis of stroke. However, the causality between SELENOS and stroke risk remains poorly understood. This study aimed to explore the association between genetically determined plasma SELENOS levels and the risk of all-cause stroke, ischemic stroke, and intracerebral hemorrhage (ICH) using a two-sample Mendelian randomization (MR) approach. We analyzed data from three large-scale Genome-Wide Association Study (GWAS) meta-analyses of individuals of European descent. The fixed-effect inverse-variance weighted (IVW) model analysis revealed that genetically elevated SELENOS levels were associated with an increased risk of all-cause stroke, ischemic stroke, and ICH. Sensitivity analyses showed no evidence of pleiotropy or heterogeneity, and leave-one-out analyses confirmed the robustness of our results. Here, we show that elevated plasma SELENOS levels are causally linked to increased stroke risk. Although the effect sizes were modest, these findings suggest SELENOS may play a role in stroke pathogenesis, emphasizing the need for further mechanistic and functional studies. Finally, our findings shed light on the importance of tailored Se intake management in the context of stroke prevention.
The Correlation Between Self-Assessment and Observation in Driving Style Classification: An On-Road Case Study
Authors: Kamaludin Muhammad Zainul Abidin , Karjanto Juffrizal , Muhammad Noryani , Md Yusof Nidzamuddin , Hassan Muhammad Zahir , Baharom Mohamad Zairi , Mohd Jawi Zulhaidi , Rauterberg Matthias
Published: February 2025

A typical classification of driving style from a human driver is conducted via self-assessment, which begs the question of the possibility of bias from the respondents. Although some research has been carried out validating the questionnaire, no controlled studies have yet to be reported to validate the Malaysian driving style. This study aimed to validate the Malaysian driver using the Multidimensional Driving Style Inventory (MDSI) with five-factor driving styles (careful, risky, angry, anxious, and dissociative) in on-road situations. Forty-one respondents completed the experiment on two designated routes recorded over 45 min of driving. A modest correlation existed between the MDSI and the score retrieved from the on-road observation assessment. The result showed a low-to-medium correlation collected from acceleration in longitudinal directions compared with correlation analysis utilizing the MDSI scale. Exploring such latent traits is essential for precisely classifying human driver styles without bias.
Molecular Detection of Hemoparasites in Hematophagous Insects Collected from Livestock Farms in Northeastern Thailand
Authors: Jhaiaun Pairpailin , Rudeekiatthamrong Apiraya , Chimnoi Wissanuwat , Nguyen Giang Thi , Ngasaman Ruttayaporn , Phasuk Jumnongjit , Kamyingkird Ketsarin
Published: February 2025

Vector-borne parasitic diseases (VBPDs) are diseases that have a major impact on the health and productivity of livestock. VBPDs, including trypanosomosis, theileriosis, and anaplasmosis, are caused by blood parasites and Rickettsiales infections that can be transmitted by biting insects. The control and elimination of VBPDs is difficult and challenging. This study contributed to better understanding the impact of hematophagous insects on carrying pathogens to livestock by investigating the detection of Trypanosoma evansi, Theileria spp. and Anaplasma spp. in hematophagous insects collected from five livestock farms in Northeastern Thailand using polymerase chain reaction (PCR). The Stomoxys and Tabanus samples were collected using Nzi and Vavoua traps. Morphological and molecular identifications of hematophagous insects were performed. The Stomoxys samples were dissected into head and abdomen parts, while the Tabanus samples were dissected into head, salivary glands, and abdomen parts before DNA extraction individually. The host blood meal in the DNA of each part of the hematophagous insects was identified using PCR targeting the prepronociceptin (PNOC) gene. PCR detection of T. evansi, Theileria spp., and Anaplasma spp. were performed by targeting the ITS2, 18s rRNA, and msp4 genes, respectively. In total, 131 insect samples were collected, with Stomoxys calcitrans being the most common species at 40 (30.53%), followed by Tabanus spp. at 14 (10.69%) and the other Stomoxys spp. at 7 (5.34%), respectively. The hemoparasite detection results identified Theileria spp. in the abdomen (21.43%; 3/14) of three Tabanus samples. Neither Anaplasma spp. nor T. evansi were detected in this study. Additionally, host blood meal DNA was found in the abdomen of Stomoxys calcitrans (4.17%; 1/24) and Stomoxys spp. (20%; 1/5). For the Tabanus spp., host blood meal DNA was detected in the head (7.14%; 1/14), salivary glands (7.14%; 1/14), and abdomen (14.29%; 2/14). These results highlighted that Tabanus spp. might play an important role in Theileria transmission.
The Impact of Termiticides on Termite Corpse Management
Authors: Shi Jizhe , Merchant Austin , Zhou Xuguo
Published: February 2025

Soil treatments and baits are two primary chemical control strategies for subterranean termites. Baiting is targeted and eco-friendly but requires ongoing maintenance, while soil treatments provide immediate, long-lasting protection with potential environmental concerns. Previously, we found that termites differentially manage deceased individuals based on their postmortem chemical signatures, potentially circumventing chemical controls. Given the distinct differences in the synthetic termiticides used for soil treatments (fast-acting) and baits (slow-releasing), we hypothesized that termites would respond differently to corpses treated with these two methods. To test this hypothesis, in Reticulitermes flavipes, we (1) profiled postmortem chemicals in termites exposed to different termiticides and (2) documented live termite responses to these corpses. Significant variations in postmortem chemical signatures, particularly 3-octanol and 3-octanone, were found among termites exposed to different termiticides, especially bifenthrin and fipronil. However, these variations did not lead to significantly different undertaking behaviors, indicating a complex relationship between death cues and termite behavior. Contrary to our hypothesis, except for bifenthrin, the fundamental undertaking behaviors were consistent despite differences in retrieval timing. This suggests that termiticides alone do not fully dictate termite undertaking behavior. Understanding termite corpse management is crucial for evaluating termiticide effectiveness, highlighting the need for an integrated pest management approach.
Changes in the Concentration Profile of Selected Micro- and Macro-Elements in the Yellow Ligament Obtained from Patients with Degenerative Stenosis of the Lumbo-Sacral Spine
Authors: Strojny Damian , Sobański Dawid , Wojdyła Roman , Skóra Klaudia , Hoczela Martyna , Wyczarska-Dziki Katarzyna , Miller Mateusz , Masternak Mateusz , Staszkiewicz Rafał , Wieczorek Jerzy , Wieczorek-Olcha Weronika , Waltoś-Tutak Barbara , Gogol Paweł , Grabarek Beniamin Oskar
Published: February 2025

Background/Objectives: Degenerative lumbo-sacral spinal stenosis is characterized by spinal canal narrowing, often linked to ligamentum flavum hypertrophy. This study evaluated the elemental composition of ligamentum flavum tissue in DLSS patients compared to healthy controls. Methods: This study involved 180 patients diagnosed with degenerative lumbo-sacral spinal stenosis and 102 healthy controls. Ligamentum flavum samples were analyzed for concentrations of magnesium (Mg), calcium (Ca), phosphorus (P), zinc (Zn), copper (Cu), iron (Fe), sodium (Na), potassium (K), manganese (Mn), and lead (Pb) using inductively coupled plasma optical emission spectrometry (ICP-OES). Statistical analyses were conducted using Student’s t-test, ANOVA, and Pearson’s correlation, with a significance threshold of p < 0.05. Results: The study group exhibited significantly elevated levels of Mg (p < 0.001), Ca (p = 0.014), and P (p = 0.006), along with reduced concentrations of Zn (p = 0.021) and Cu (p = 0.038) compared to controls. No statistically significant differences were observed for Na, K, Mn, or Fe (p > 0.05). Elemental imbalances were more pronounced in individuals with higher body mass index (BMI) and varied by gender. Pain intensity demonstrated a significant correlation with Zn (p = 0.012) and Na (p = 0.045), but no consistent associations with Mg, Ca, or P. Conclusions: Altered Mg, Ca, P, and Zn levels in ligamentum flavum suggest their involvement in degenerative lumbo-sacral spinal stenosis pathophysiology. These elements may serve as potential biomarkers and therapeutic targets for mitigating spinal canal narrowing.
Unveiling the Role of Selenium in Child Development: Impacts on Growth, Neurodevelopment and Immunity
Authors: Batyrova Gulnara , Taskozhina Gulaim , Umarova Gulmira , Umarov Yeskendir , Morenko Marina , Iriskulov Bakhtiyar , Kudabayeva Khatimya , Bazargaliyev Yerlan
Published: February 2025

Selenium (Se) is a vital trace element for children, playing a crucial role in numerous physiological processes, including antioxidant defense, immune regulation, thyroid function, and bone metabolism. Emerging evidence highlights its potential impact on child development and growth while also underscoring the complexity of its mechanisms and the global variations in Se intake. The aim of this review is to comprehensively elucidate the significance of Se in various biological processes within the human body, with a focus on its role in child development and growth; its biochemical effects on the nervous system, thyroid function, immune system, and bone tissue; and the implications of Se deficiency and toxicity. This review integrates findings from experimental models, epidemiological studies, and clinical trials to explore Se’s role in neurodevelopment, growth regulation, and immune competence in children. Selenoproteins, which regulate oxidative stress and thyroid hormone and bone metabolism, are essential for normal growth and cognitive development in children. Se deficiency and toxicity has been linked to impaired immune function, growth retardation, and decreased immune function. The findings underscore Se’s influence on various biological pathways that are critical for healthy child development and its broader importance for child health. Public health strategies aimed at optimizing selenium intake may play a pivotal role in improving pediatric health outcomes worldwide.
Effect of Oral + Topical and Only Topical Tranaxamic Acid Application on Blood Loss and Postoperative Transfusion in Primary Total Hip Arthroplasty
Authors: Mutlu Tansel , Arıcan Mehmet , Karaduman Zekeriya Okan , Turhan Yalçın , Kaban İlyas , Dalaslan Raşit Emin , Sağlam Sönmez , Yücel Mücahid Osman
Published: February 2025

Objectives: Total hip arthroplasty is one of the most common procedures performed to reduce pain and improve hip functions in patients with advanced hip osteoarthritis, but perioperative blood loss, acute anemia and transfusion requirement increase the risk of morbidity and mortality during and after surgery and negatively affect functional recovery. We aimed to present the comparative results of oral + topical and only topical tranexamic acid application to reduce blood loss and postoperative transfusion in primary total hip arthroplasty. Methods: We retrospectively evaluated the patients who applied to the Orthopedics and Traumatology outpatient clinic with complaints of hip pain and limited movement between January 2014 and December 2020, who underwent primary total hip arthroplasty with the diagnosis of coxarthrosis and who were administered oral + topical and only topical tranexamic acid before and during surgery, in terms of blood loss and transfusion requirement. Results: No statistically significant difference was observed between the preoperative, day 0 and day 1 hemoglobin means in those that were applied oral + topical tranexamic acid and those that were applied only topical (p > 0.05). However, the second- and third-day hemoglobin means in those that were treated with topical medication alone were found to be statistically significantly lower than in those that were treated with oral + topical tranexamic acid (p = 0.032, p = 0.0001). Conclusions: Oral + topical tranexamic acid application in total hip arthroplasty surgery is more effective than topical applications alone when it comes to reducing blood loss, hemoglobin and hematocrit decrease without increasing the risk of thromboembolic diseases and wound complications.
Marine Radar Target Ship Echo Generation Algorithm and Simulation Based on Radar Cross-Section
Authors: Li Chang , Yang Xiao , Ren Hongxiang , Li Shihao , Feng Xiaoyu
Published: February 2025

In this study, a simplified radar echo signal model suitable for radar simulators and a Radar Cross-Section (RCS) calculation model based on the Physical Optics (PO) method was developed. A comprehensive radar target ship echo generation algorithm was designed, and the omnidirectional radar RCS values of three typical ships were calculated. The simulation generates radar target ship echo images under varying incident angles (0–360°), detection distances (0–24 nautical miles), and three common target material properties. The simulation results, compared with those from existing radar simulators and real radar systems, show that the method proposed in this study, based on RCS values, generates highly realistic radar target ship echoes. It accurately simulates radar echoes under different target ship headings, distances, and material influences, fully meeting the technical requirements of the STCW international convention for radar simulators.
Typhoon Blend Wind Field Optimization Using Wave-Height Hindcasts
Authors: Chen Tzu-Chieh , Hu Kai-Cheng , Wu Han-Lun , Lu Wei-Shiun , Chen Wei-Bo , Chiang Wen-Son , Hsiao Shih-Chun
Published: February 2025

Typhoons cause significant losses and pose substantial threats every year, with an increasing trend observed in recent years. This study evaluates significant wave height (SWH) hindcasts for typhoons affecting Taiwan using optimized wind field configurations within the SCHISM-WWM-III coupled model. To enhance typhoon-induced SWH simulations, the blended wind field integrates ERA5 reanalysis wind data with the modified Rankine vortex wind model. Key parameters, including the parametric wind field start time, best track data, and the radius of maximum wind speed, were carefully selected based on analyses of typhoons Meranti and Megi in 2016. Validation metrics such as the skill core, HH indicator, maximum SWH difference, and peak time difference of the SWH indicate that the optimized setup improves the accuracy of simulation. The findings highlight the effectiveness of the adjusted blended wind field, the high-resolution best track data provided by Taiwan, and the maximum wind speed radius in significantly enhancing the accuracy of typhoon wave modeling for the waters surrounding Taiwan.
A Review of the Biotechnological Potential of Cave Fungi: A Toolbox for the Future
Authors: Barbosa Renan N. , Felipe Maria Tamara C. , Silva Leticia F. , Silva Edna A. , Silva Sabrina A. , Herculano Polyanna N. , Prazeres José F. S. A. , Lima Joenny M. S. , Bezerra Jadson D. P. , Moreira Keila A. , Magalhães Oliane M. C. , Souza-Motta Cristina M.
Published: February 2025

The study of the intersection between biodiversity and biotechnology has revealed a rich source of innovations. Fungi, with their vast range of morphologies and lifestyles, thrive in various habitats, including caves. With impressive metabolic characteristics, they play a key role in producing essential biotechnological compounds for various economic sectors. This paper aims to consolidate evidence on the biotechnological potential of fungi isolated from caves, highlighting the urgency of conserving and exploring these ecosystems. For this purpose, we conducted a comprehensive literature search using scientific databases (SciELO, Medline Complete, Medline/PubMed, Web of Science, Scopus (Elsevier), and Google Scholar). We adopted an interdisciplinary approach by collecting information from 22 papers published between 2013 and 2024. Based on these data, our survey revealed broad potential, including antimicrobial compounds, antioxidants, antitumor agents, enzymes, and organic acids. We emphasize that accurately identifying and depositing fungal isolates in reference collections are crucial for reliable research and effective industrial applications, driving metabolic bioactivity and the production of substances with the potential to inhibit pathogens. Conserving and protecting the cave environment is imperative, considering its continuous potential for discovery and contribution to scientific advancement.
The Importance of Urban Greening Spaces for Avian Communities in an Urbanized Landscape
Authors: Kopij Grzegorz
Published: February 2025

Island ecology plays an important role in explaining various ecological and evolutionary processes. Small, isolated oceanic islands, exemplified by the Azores Archipelago, are especially vulnerable to adverse environmental conditions and human impact. The study aims to evaluate the impact of urbanization, especially the urban greening space, on the structure and dynamics of avian communities associated with various landforms in an urbanized landscape in one of the nine islands of the Azores Archipelago, São Miguel Island, in the northeast Atlantic Ocean. Samples were collected in the second half of April 2024. The line transect method (43 transects with a total of 37.4 km) was employed to count all bird species breeding in different landforms distinguished in the city: coastal land, urbanized land, rural land, and urban greening space. The obtained results showed that the number of breeding species was much higher in urban greening spaces (n = 20) than in the other lands (n = 10–14 species). Both cumulative dominance and dominance indices were much lower in urban greening space than in the other landforms. The Sørensen Index of Similarity between the four main land categories distinguished in the city varied between 0.62 and 0.96, being the lowest between the coastal and urban greening space, and the highest between the urbanized and rural lands. Two main feeding guilds were distinguished in the study area: granivores and insectivores. The former guild clearly dominated over the latter in all major land categories distinguished. Clearly, the proportion of granivores increased with urbanization. Also, two nesting guilds were distinguished: buildings and trees/shrubs. The former was dominant in all land categories except for the urban greening space where the tree/shrub nesting guild was more numerous than the building guild. The tree/shrub guild declined with urbanization. A general trend was recorded: the higher the level of urbanization, the lower the percentage of urban greening space, and in consequence, the lower the number of bird species and diversity indices, but the higher the cumulative dominance and dominance indices. The overall density of birds remains, however, distinctively similar. In the future, data on the population densities and dominance of particular species should be collected in urbanized landforms in other towns of the Azores Archipelago and Macaronesia at large.
Candidate Genetic Modifiers in Alport Syndrome: A Case Series
Authors: Lujinschi Ștefan Nicolaie , Sorohan Bogdan Marian , Obrișcă Bogdan , Vrabie Alexandra , Rusu Elena , Zilișteanu Diana , Achim Camelia , Andronesi Andreea Gabriella , Ismail Gener
Published: February 2025

Background: Alport syndrome (AS) is one of the most common monogenic kidney disorders. Recent studies have highlighted the modifier effect of variants involving podocyte and non-collagenous extracellular matrix (ECM) proteins in AS. Methods: We report a case series of eight patients with genetically proven AS and simultaneous variants involving podocyte and non-collagenous ECM proteins. Our aim is to describe the influence of such variants on the phenotype of patients with AS. Results: We identified 10 different type IV collagen variants. Patients were diagnosed with autosomal dominant (3/8), autosomal recessive (2/8), digenic (2/8) and X-linked AS (1/8). There were eight different variants involving podocyte and non-collagenous ECM proteins. The genes involved were CRB2, LAMA5, LAMB2, NUP107, MYO1E and PLCE1. Four patients (LAMB2, LAMA5 and PLCE1 variants) presented with nephrotic syndrome or nephrotic range proteinuria. Two patients had hearing loss. Most patients (7/8) had a family history of kidney disease. Two patients (LAMB2 and LAMA5 variants) were diagnosed with focal segmental glomerulosclerosis. Two patients developed end-stage kidney disease (LAMA5, MYO1E and NUP107 variants). Conclusions: Although mutations of podocyte and ECM proteins do not have phenotypic expression in monoallelic form, the presence of such variants could explain the phenotypic variability of AS.