bluuebunny/crossref_metadata_2025 huggingface dataset:
PELAYANAN KTP-EL DI KECAMATAN CILAWU KABUPATEN GARUT DI MASA PANDEMI COVIDǧ19
Authors: Suratha I Gede , Akbar Gesha Ilyasa , Sukmahandika Yanuar , Yuvianti Nabila Brigaputri
Published: August 2024

Dokumen kependudukan merupakan sebuah dokumen utama, bagi warga Masyarakat yang telah berusia 17 tahun keatas, atau yang sudah menikah dan pernah menikah, sehingga dinas DUKCAPIL dimasa pandemic covid 19, walaupun adanya keterbatasan-keterbatasan tetap melakukan pelayanan kepada Masyarakat secara baik, khususnya pelayanan kartu tanda penduduk. Untuk itu peneliti tertarik melakukan penelitian dengan judul Pelayanan KTP el Di Kecamatan Cilawu Kabupaten Garut dimasa pandemic covid 19. T ujuan dari penelitian ini adalah sejauhmana pelayanan publik yang dilakukan oleh Dinas DUKCAPIL Kabupaten Garut, dimasa pandemic covid 19. Sehingga Masyarakat merasakan terlayani dengan sebaik baiknya tanpa kendala apapun. Khususnya pada pelayanan KTP-el, di Kecamatan Cilawu oleh Dinas DUKCAPIL Kabupaten Garut. Penelitian ini menggunakan metode kualitatif dengan analisis diskriptif, sumber data yang diperoleh dalam penelitian ini adalah bersumber dari data sekunder dan data primer, Hasil dan simpulan, bahwa pelayanan yang dilakukan dengan mekanisme secara on-line, dalam arti Masyarakat tidak harus mendatangi kantor kecamatan untuk mendapatkan pelayanan dari jajaran petugas kecamatan, cukup dilakukan dengan mengup-load dokumen-dokuem yang diminta secara on-line, setelah dokumen diterima, baru Masyarakat datang secara bergantian sesuai jadwal, untuk melakukan pemotretan biometric, khusus pelayanan Kartu Tanda Penduduk KTP-el. Ini dilakukan untuk mencegah adanya penularan secara langsung semakin massif covid 19 di Kecamatan Cilawu. Dan dapat juga pelayanan dilakukan dengan system jemput bola, namun cara ini sering mengalami hambatan-hambatan akibat jaringan yang masih sangat sulit, karena kondisi wilayah Kecamatan Cilawu tidak semua datar, tetapi berada pada kondisi lokasi yang berbukit-bukit. Namun pelayanan tetap dilakukan dan Masyarakat merasakan kepuasannya. Kata Kunci : Kartu Tanda Penduduk Elektronik; Pelayanan; Pandemi Covid-19
INOVASI PELAYANAN PENDAFTARAN PENDUDUK DI KABUPATEN GARUT DALAM MASA PANDEMI COVID 19
Authors: Moersito Joko , Rossy Fahrul , Relly Fatihah Khairunnisa , Warikar Jimmy Stevanus Samuel
Published: August 2024

Sulitnya Masyarakat mendapatkan pelayanan publik dimasa pandemic covid 19, yang diakibatkan adanya pembatasan-pembatasan kegiatan Masyarakat, sehingga dinas DUKCAPIL Kabupaten Garut, dimasa pandemic covid 19 dalam melakukan pelayanan kepada Masyarakat, perlu melakukan inovasi-inovasi pelayanan yang dapat diterima atau diberikan kepada Masyarakat, untuk itu peneliti tertarik melakukan penelitian pada dinas DUKCAPIL Kabupaten Garut, tentang Inovasi Pelayanan Pendaftaran Penduduk di Kabupaten Garut dimasa pandemic covid 19 “. Tujuan dari penelitian ini adalah, dimana peneliti ingin mengetahui bagaimana bentuk pelayanan yang dapat dilakukan dinas DUKCAPIL dimasa pandemic covid 19, khususnya menyangkut dokumen kependudukan di Kabupaten Garut. Metode yang digunakan dalam penelitian ini adalah metode kualitatif diskriptif, dengan pendekatan dan analisis diskriptif ini, dan sumber data diperoleh dari kajian literature, dokumentasi-dokumentasi dan informasi yang diterima dari hasil wawancara dengan berbagai informan terkait dengan pelayanan pada dinas DUKCAPIL Kabupaten Garut dimasa pandemic covid 19. Hasil dan simpulan, bahwa pelayanan dilakukan dengan system on-line dimana Masyarakat tidak datang kekantor DUKCAPIL secara langsung, tetapi menggunakan aplikasi on-line yang telah disiapkan oleh kantor DUKCAPIL Kabupaten Garut. Hal ini untuk menghindari adanya penularan yang semakin massif covid 19 di Kabupaten Garut.Kata kunci: Inovasi, Pandemi Covid-19, Pelayanan Pendaftaran Kependudukan Kabupaten Garut
Resección de tumor retroperitoneal en el Hospital Nacional Arzobispo Loayza
Authors: Calle-Brush Alejandro , Adams-Hurtado Eduardo , Querevalú-Olivares Alan , Reyes-Hernández Néstor
Published: August 2024

Reportamos el caso de un paciente varón de 54 años con dolor abdominal asociado a distensión y llenura precoz a causade un tumor retroperitoneal gigante, el cual se resolvió luego de la resección total de éste. El paciente presentó la particularidad de tener una función renal disminuida del riñón contralateral al tumor por un cuadro de litiasis renal, por lo cual preservar el riñón derecho era vital y se logró con una disección minuciosa del tumor. El paciente fue dado de alta sin intercurrencias en el PO5, y al momento continúa con buena evolución, esperando resultados de inmunohistoquímica del tumor.
Students' Appreciation of Indonesian Language Learning During Online
Authors: Pratikno Heru , Atsani Wulansari
Published: August 2024

In its development, Indonesian has played a central role in the world of education in the country, from basic education to higher education. As proof, Indonesian is used as the language of instruction in learning. In addition, Indonesian functions as a tool for developing science and technology. With the presence of such a language, it can actually become a strong capital in moving towards an even greater Indonesian nation. However, what does that mean if the users, especially the younger generation, namely students, don't care about their own language. This can be reflected when they take Indonesian language courses online. Therefore, the aim of this research is to explain what problems students have in learning Indonesian at Islamic universities. Apart from that, the author tries to find the best solution to solve this problem so that it is resolved. The method used in this paper is direct observation of the behavior of students studying Indonesian online. The hypothesis is that there is a strong correlation between lecturers and students to create a comfortable Indonesian language lecture atmosphere.
The Role of the Environment in Human Life in the Novels Tambora 1815 and Medulla Sinculasis by Paox Iben Mudhaffar
Authors: Hendri  , Wahidatul Murtafi’ah
Published: August 2024

The purpose of this research is to describe (1) the form of environmental role in human life, (2) the form of human attitude to the environment, (3) the form of local wisdom in its relation with environment in novel Medulla Sinculasis and Tambora 1815 by Paox Iben Mudhaffar. This research was qualitative descriptive. The source of data  was the novels Medulla Sinculasis and Tambora 1915 by Paox Iben Mudhaffar. The collection of the data used document content analysis (read-note-analysis). Analysis of the data in this study is a qualitative descriptive analysis technique is done by categorizing, describing, and conclusion.  In this research, the researchers chose the novels Medulla Sinculasis and Tambora 1815 as data sources considering that these novels were the most appropriate to study among Paox Iben's other works. The Tambora 1815 novel used is the first printed novel, March 2015 published by Bukudaku Addressra Publishing, Mataram with a book thickness of 301 pages, ISBN: 978-979-18863-1-4 and the Medulla Sinculasis novel used is the first printed novel, January 2011 published by IruS (Rumah Arus Institute) Mataram, Lombok with a book thickness of 324 pages, ISBN: 979-1698-045-94-7. The results of this research are as follows. First, the role of environment represented in the novels Medulla Sinculasis and Tambora 1815  consists of : (a) the environment as the source of food, (b) the environment as the source of medicines, (c) the environment as the source of building material, (d) the environment as living place, (e) the environment as the provider of payment tool (barter), (f) the environment as a means of livelihood, (g) the environment as the source of water, (h) the  environment as the provider of things for means of transportation, and (i) the environment as tourist object.
STRATEGI DAKWAH HABIB ALWI BIN MUHAMMAD AL ATTHOS DALAM MEMBENTUK AKHLAK PEMUDA DI KOTA BEKASI
Authors: Dalimunthe Achmad Nurfadillah , Malayati Robi’ah Machtumah
Published: August 2024

Penelitian ini mengeksplorasi dua masalah utama: (1) Bagaimana Habib Alwi menggunakan strategi dakwah untuk membentuk akhlak pemuda di Kota Bekasi? dan (2) Bagaimana pendekatan Habib Alwi dalam membentuk akhlak pemuda di Kota Bekasi? Penelitian ini menggunakan pendekatan kualitatif deskriptif dengan teknik wawancara, observasi dan dokumentasi. Implikasi dari penelitian ini meliputi strategi dakwah Habib Alwi dalam membentuk akhlak pemuda di Kota Bekasi, akar permasalahan pemuda di Kota Bekasi, dan respons pemuda Bekasi terhadap dakwah yang dilakukan oleh Habib Alwi. Hasil analisis menunjukkan bahwa: (1) dakwah Habib Alwi Bin Muhammad Al Atthos menggunakan strategi bil-hal dengan memperhatikan juga ajaran bil-lisan, dan (2) pendekatan dakwah Habib Alwi Wadah Muhammad Al Atthos dalam membentuk karakter pemuda di Kota Bekasi adalah dengan menonjolkan kualitas keteladanan Nabi Muhammad SAW melalui interaksi yang meyakinkan dan menyenangkan untuk merangkul serta menginspirasi pemuda Bekasi tersebut.
Perancangan dan Implementasi Sistem Informasi Launchpad Cryptocurrency pada PT. Pintar Media Teknologi
Authors: Suwarno Suwarno , Valentino Elvin
Published: August 2024

Teknologi blockchain telah menjadi fokus utama dalam pengembangan aplikasi web terdesentralisasi, membuka era baru dalam evolusi internet yang dikenal sebagai Web3. Di tengah perkembangan ini, perusahaan startup PT. Pintar Media Teknologi memperkenalkan aplikasi launchpad cryptocurrency yang mengandalkan teknologi blockchain untuk memberikan solusi terdesentralisasi dalam perdagangan aset crypto. Penulis mendapatkan kesempatan untuk melakukan penelitian pada PT. Pintar Media Teknologi untuk membangun aplikasi launchpad cryptocurrency. Metodologi pengembangan aplikasi blockchain yang diterapkan adalah metodologi Agile Scrum. Melalui analisis fitur-fitur yang dikembangkan, seperti perancangan Entity Relationship Diagram, desain basis data, hingga implementasi fitur-fitur utama seperti autentikasi menggunakan Web3, manajemen token, dan manajemen launchpad. Hasil penelitian ini memberikan wawasan tentang praktik pengembangan aplikasi blockchain dan kontribusi penulis pada penelitian ini adalah merancang dan mengimplementasi sistem informasi launchpad cryptocurrency sesuai dengan kebutuhan para stakeholders sehingga dapat  mempercepat proses pengembangan produk.
Fungi-sensitized individuals have unique profiles where Alt a 1 dominates promoting response to grass, ragweed and cat allergens
Authors: Kalyniuk Viktoriia , Rodinkova Victoria , Yuriev Serhii , Mokin Vitalii , Losenko Arsen , Kryvopustova Mariia , Zabolotna Diana , Gogunska Inna
Published: August 2024

Introduction

The aim of our work was to determine comprehensively the sensitization profile of patients hypersensitive to fungal allergenic components in the Ukrainian population, identifying features of their co-sensitization to allergens of other groups and establishing potential relationships between causative allergens and their ability to provoke this hypersensitivity.

Methods

A set of programs was developed using Python and R programming languages, implementing the K-means++ clustering method. Bayesian networks were constructed based on the created clusters, allowing for the assessment of the probabilistic interplay of allergen molecules in the sensitization process of patients.

Results and discussion

It was found that patients sensitive to fungi are polysensitized, with 84.77% of them having unique allergological profiles, comprising from 2 to several dozen allergens from different groups. The immune response to Alt a 1 may act as the primary trigger for sensitization to other allergens and may contribute to a high probability of developing sensitivity to grasses (primarily to Phl p 2), ragweed extract, and the Amb a 1 pectate lyase, as well as to pectate lyase Cry j 1 and cat allergen Fel d 1. Individuals polysensitized to molecular components of fungi were often sensitive to such cross-reactive molecules as lipocalins Fel d 4 and Can f 6, as well. Sensitivity to Ambrosia extract which dominated in the development of sensitization to ragweed pollen indicating the importance of different allergenic components of this plant's pollen. This hypothesis, along with the assumption that Phl p 2 may be the main trigger for sensitivity to grasses in patients with Alternaria allergy, requires further clinical investigation.
Negativity of factor correlations biases the sizes of factor variances in bifactor CFA models
Authors: Schweizer Karl , Ren Xuezhu , Wang Tengfei
Published: August 2024

Estimates of factor variances observed together with negative factor correlations in CFA using the bifactor model are explored for specific characteristics. The analysis is conducted on the basis of quantified accounts of common systematic variation achieved by the two group latent variables of the model. It reveals that negative factor correlations tend to be associated with larger factor variance estimates than the zero correlation and positive correlations. Further, it reveals that upper limits to the sizes of factor variances for positive factor correlations corresponding to expectations exist while in negative correlations such limits are missing and allow for overly large factor variance estimates. Results of the analysis based on quantified accounts are supported by the results of a simulation study.
Synthesis of BODIPY FL-tethered ridaifen-B, RID-B-BODIPY, and its localization in cancer cells
Authors: Murata Takatsugu , Komukai Kyoka , Semba Yuta , Murata Eri , Sato Fumi , Takano Tomohiro , Tsuchiya Kaho , Matsuda Chihiro , Sakai Anju , Yoneoka Amane , Takahashi Shunsuke , Nagahara Yukitoshi , Shiina Isamu
Published: August 2024

We synthesized ridaifen-B boron dipyrromethene (RID-B-BODIPY) using 2-methyl-6-nitro benzoic anhydride (MNBA)-mediated dehydration condensation reaction between amino alkyl-tethered RID and BODIPY FL. Comparative experiments between dicyclohexylcarbodiimide (DCC) and MNBA for their coupling reactions demonstrated that MNBA is an effective condensation reagent for amines and BODIPY FL. A cell staining study with RID-B-BODIPY showed intracellular localization of BODIPY FL fluorescence, attributed to the RID-B structure, indicating the successful development of a tool for analyzing intracellular molecular behavior efficiently.
Water filter: a rapid water environmental DNA collector in the field
Authors: Wu Ping , Feng Jie , Ju Mingxia , Wu Shenhao , Han Weichun , Wang Miao , Liao Junquan , Zhao Lifeng , Gao Yifan , Zheng Jiao , Luo Mingjie , Gong Huixian , Zeng Lidong , Lai Juan , Li Mingze , Yan Qin , Sun Lei , Liu Yongfeng
Published: August 2024

Biological monitoring using environmental DNA (eDNA) technology has expanded from micro- to macro-organisms. In aquatic eDNA studies, large volumes of water need to be filtered rapidly in the field, which requires development of effective eDNA collection devices. In this study, we introduce a novel portable eDNA collection system containing a GM dual-channel water filter and a DNA extraction kit adapted to large filter membranes (ø 100 mm). The water filter is powered by a high-capacity lithium battery (9,000 mA), which operates two peristaltic pumps and maintains a continuous filtration rate of up to 1 L/min for 5 h in outdoor settings. For sample collection, the optimum conditions are still water and turbidity below 8 nephelometric turbidity units. This allows for the filtration of 10 L of water within 10 min by use of a 0.22-μm filter. Metagenomic and 12S metabarcoding sequencing showed that the DNA extraction quality and species annotation accuracy of our custom DNA extraction kit, which was tailored for this system, rivaled the performance of established kits. The GM water filter’s enrichment mode gave consistent results with vacuum filtration, which greatly reduced the filtration time for large water samples, while accurately reproducing species annotations. This innovation streamlines the eDNA collection and annotation process and offers substantial benefits for biodiversity monitoring and conservation efforts.
Advances in the application and mechanism of bioactive peptides in the treatment of inflammation
Authors: Liu Haiyang , Zhang Lulu , Yu Jingmou , Shao Shengwen
Published: August 2024

Inflammation is a normal immune response in organisms, but it often triggers chronic diseases such as colitis and arthritis. Currently, the most widely used anti-inflammatory drugs are non-steroidal anti-inflammatory drugs, albeit they are accompanied by various adverse effects such as hypertension and renal dysfunction. Bioactive peptides (BAPs) provide therapeutic benefits for inflammation and mitigate side effects. Herein, this review focuses on the therapeutic effects of various BAPs on inflammation in different body parts. Emphasis is placed on the immunomodulatory mechanisms of BAPs in treating inflammation, such as regulating the release of inflammatory mediators, modulating MAPK and NF-κB signaling pathways, and reducing oxidative stress reactions for immunomodulation. This review aims to provide a reference for the function, application, and anti-inflammation mechanisms of BAPs.
HMGB2 drives tumor progression and shapes the immunosuppressive microenvironment in hepatocellular carcinoma: insights from multi-omics analysis
Authors: Chen Yan-zhu , Meng Zhi-shang , Xiang Zuo-lin
Published: August 2024

Background

Hepatocellular carcinoma (HCC) poses a significant health burden globally, with high mortality rates despite various treatment options. Immunotherapy, particularly immune-checkpoint inhibitors (ICIs), has shown promise, but resistance and metastasis remain major challenges. Understanding the intricacies of the tumor microenvironment (TME) is imperative for optimizing HCC management strategies and enhancing patient prognosis.

Methods

This study employed a comprehensive approach integrating multi-omics approaches, including single-cell RNA sequencing (scRNA-seq), bulk RNA sequencing (Bulk RNA-seq), and validation in clinical samples using spatial transcriptomics (ST) and multiplex immunohistochemistry (mIHC). The analysis aimed to identify key factors influencing the immunosuppressive microenvironment associated with HCC metastasis and immunotherapy resistance.

Results

HMGB2 is significantly upregulated in HCCTrans, a transitional subgroup associated with aggressive metastasis. Furthermore, HMGB2 expression positively correlates with an immunosuppressive microenvironment, particularly evident in exhausted T cells. Notably, HMGB2 expression correlated positively with immunosuppressive markers and poor prognosis in HCC patients across multiple cohorts. ST combined with mIHC validated the spatial expression patterns of HMGB2 within the TME, providing additional evidence of its role in HCC progression and immune evasion.

Conclusion

HMGB2 emerges as a critical player of HCC progression, metastasis, and immunosuppression. Its elevated expression correlates with aggressive tumor behavior and poor patient outcomes, suggesting its potential as both a therapeutic target and a prognostic indicator in HCC management.
Protocol for a systematic review and individual participant data meta-analysis of optimizing oxygen therapy in critically ill patients
Authors: Yang Xiaobo , Ouyang Yaqi , Xu Jiqian , Shang You
Published: August 2024

Background

Oxygen therapy is a cornerstone treatment of critically ill patients in the intensive care unit (ICU). Whether lower oxygenation therapy brings superior survival outcomes to higher oxygenation therapy is unknown.

Methods

We will search electronic databases: PubMed, Embase, Web of Science, the Cochrane Central Register of Controlled Trials (CENTRAL), International Clinical Trials Registry Platform (ICTRP), and ClinicalTrials.gov from inception to 1 January 2024. Two authors will independently screen for all eligible clinical studies. Emails will be sent for individual participant data. The statistical analyses will be conducted using STATA 15.0 software.

Results

We will evaluate the efficacy of lower oxygenation therapy compared with higher oxygenation therapy based on individual participant data.

Conclusion

This study will offer clinical evidence for oxygen therapy in ICU patients.
Discrimination training affects stimulus generalization in mice during Pavlovian eyeblink conditioning
Authors: Fiocchi Francesca Romana , van Dorp Nikki E. S. , Dijkhuizen Stephanie , van den Berg Maurits , Wong Aaron , De Zeeuw Chris I. , Boele Henk-Jan
Published: August 2024

The delicate balance between discrimination and generalization of responses is crucial for survival in our ever-changing environment. In particular, it is important to understand how stimulus discrimination affects the level of stimulus generalization. For example, when we use non-differential training for Pavlovian eyeblink conditioning to investigate generalization of cerebellar-related eyelid motor responses, we find generalization effects on amount, amplitude and timing of the conditioned responses. However, it is unknown what the generalization effects are following differential training. We trained mice to close their eyelids to a 10 kHz tone with an air-puff as the reinforcing stimulus (CS+), while alternatingly exposing them to a tone frequency of either 4 kHz, 9 kHz or 9.5 kHz without the air-puff (CS−) during the training blocks. We tested the generalization effects during the expression of the responses after the training period with tones ranging from 2 kHz to 20 kHz. Our results show that the level of generalization tended to positively correlate with the difference between the CS+ and the CS− training stimuli. These effects of generalization were found for the probability, amplitude but not for the timing of the conditioned eyelid responses. These data indicate the specificity of the generalization effects following differential versus non-differential training, highlighting the relevance of discrimination learning for stimulus generalization.
Effect of green banana and pineapple fibre powder consumption on host gut microbiome
Authors: Chong Chun Wie , Liew Mei Shan , Ooi Weitze , Jamil Hassan , Lim Angie , Hooi Suet Li , Tay Clarisse S. C. , Tan Gwendoline
Published: August 2024

Purpose

To determine whether green banana powder (GBP) and pineapple fibre powder (PFP) promote beneficial bacterial species, directly improve human gut health and modulate the gut microbiome and understand their utility as functional foods and dietary supplements.

Methods

Over 14 days, 60 adults followed protocol requirements, completed food diaries and study questionnaires, avoided consuming supplements with prebiotics, probiotics or postbiotics, and ingested food containing 5 g of total daily fibre [placebo (10.75 g), GBP (10.75 g) or PFP (7.41 g)]. Participants’ medical and baseline wellness histories, as well as stool samples, were collected at baseline, day 7 and 14. Stool DNA was processed for sequencing.

Results

Dietary fibre and resistant starches (RS) in GBP and PFP promoted temporal increases in beneficial bacteria. GBP significantly elevated 7 species (F. prausnitzii, B. longum, B. bifidum, B. adolescentis, B. pseudocatenulatum, B. obeum, and R. inulinivorans), while PFP enriched 6 species (B. ovatus, B. cellulosilyticus, B. bifidum, B. intestinalis, R. inulinivorans, and E. siraeum). These bacteria, found to be deficient in younger adults, were promoted by both powders. PFP benefitted both genders aged 16–23, while GBP benefitted overweight/obese individuals, including females. GBP and PFP fiber and RS improved bowel regularity and health as well as metabolism by promoting histidine, branched-chain amino acids, short-chain fatty acids, and biotin production. The additional fiber caused “low” bloatedness and reduced “fairly bad” sleep disruptions, without affecting sleep durations.

Conclusion

GBP and PFP supplementation increased beneficial bacteria and metabolites, improved host gut health, and present a valuable nutritional strategy for enhancing human health.

Clinical trial registration

AMILI Institutional Review Board, Identifier 2023/0301.
Acetylcytidine modification of DDX41 and ZNF746 by N-acetyltransferase 10 contributes to chemoresistance of melanoma
Authors: Wang Li , Zeng Yuefen , Zhang Ying , Zhu Yun , Xu Shuangyan , Liang Zuohui
Published: August 2024

Background

Rapidly developed chemoresistance to dacarbazine (DTIC) is a major obstacle in the clinical management of melanoma; however, the roles and mechanisms of epi-transcriptomic RNA modification in this process have not been investigated.

Method

DTIC-resistant (DR) melanoma cells were established for bulk RNA sequencing. The expressions of mRNAs were detected using qRT-PCR, and protein levels were determined using Western blotting and immunohistochemistry. Acetylated RNAs were detected by dot blotting and immunoprecipitation sequencing (acRIP-seq). A lung metastasis mouse model of melanoma was established to evaluate the anti-melanoma effects in vivo.

Results

We identified that the expression of N-acetyltransferase 10 (NAT10), a catalytic enzyme for the N4-acetylcytidine (ac4C) modification of RNA, was significantly upregulated in the DR cells. Clinically, NAT10 expression was elevated in disease progression samples and predicted a poor outcome. Using ac4C RNA immunoprecipitation (ac4C-RIP), we found that the mRNAs of two C2H2 zinc finger transcriptional factors, DDX41 and ZNF746, were targets of NAT10-mediated ac4C modification. Gain- and loss-of-function experiments in NAT10, or in DDX41 and ZNF746, altered the chemosensitivity of melanoma accordingly, and the two target genes also negatively correlated with clinical outcomes. Finally, pharmacological inhibition of NAT10 with Remodelin sensitized melanoma cells to DTIC treatment in vitro and in a mouse xenograft model.

Conclusion

Our study elucidates the previously unrecognized role of NAT10-mediated ac4C modification in the chemoresistance of melanoma and provides a rationale for developing new strategies to overcome chemoresistance in melanoma patients.
Transcriptome analysis to explore the mechanism of downregulated TNIK influencing the effect of risperidone
Authors: Yuan Ruixue , Li Yaojing , Li Xiangyi , Fu Yingmei , Ning Ailing , Wang Dongxiang , Zhang Ran , Yu Shunying , Xu Qingqing
Published: August 2024

Background

Risperidone is one of the most reliable and effective antipsychotics for schizophrenia treatment. However, the mechanism of action of risperidone is not yet fully understood. Traf2 and Nck-interacting protein kinase (TNIK), a schizophrenia susceptibility gene, is associated with risperidone treatment response. Our previous in vitro experiments confirmed that downregulated TNIK affected the effect of risperidone on downstream targets. However, the effect of downregulated TNIK on risperidone-induced molecular expression remains to be further explored.

Methods

Transcriptome analysis was performed on U251 cells subjected to risperidone, TNIK siRNA, and no treatment, respectively. Compared to the no-treatment group, two groups of DEGs were screened out and then intersected with the schizophrenia-related genes to screen the cross-talk genes. Those DEGs were analyzed using GO and KEGG. STRING and Cytoscape were used to construct a protein-protein interaction (PPI) network for the cross-talk gene.

Results

The results showed that the parathyroid hormone synthesis, secretion, and action were significantly enriched after risperidone treatment. Downregulated TNIK could have an impact on the collagen-containing extracellular matrix, signaling receptor activator activity, and PI3K-Akt signaling pathway. Interestingly, bone mineralization function and calcium signaling pathway were enriched in the cross-talk genes. Additionally, FGFR2, FGF1, and FGFR might be the potential targets for TNIK affecting the effects of risperidone.

Conclusion

The study indicated that risperidone primarily influences functions and/or pathways associated with bone metabolism, potentially contributing to the adverse effect of osteoporosis. Our study may offer a novel perspective on investigating the mechanisms underlying the adverse effects of risperidone.
Comprehensive understanding of the adverse effects associated with temozolomide: a disproportionate analysis based on the FAERS database
Authors: Zhou Yusen , Jia Peng , Fang Yuting , Zhu Wei , Gong Yong , Fan Tianyu , Yin Jiangliu
Published: August 2024

Background

Temozolomide, which is the standard drug for glioma treatment, has several Adverse events (AEs) in the treatment of gliomas and other tumors that are not yet fully understood. This is due to the pharmacological nature of the alkylating agent. A significant proportion of these effects have not been systematically documented or reported.

Methods

We selected data from the United States FDA Adverse Event Reporting System (FAERS) database from the first quarter of 2004 to the fourth quarter of 2023. Four algorithms were used for disproportionate analysis, with the objective of assessing the association between temozolomide and related adverse events.

Results

In this study, 20,079,906 case reports were collected from the FAERS database, of which 15,152 adverse events related to temozolomide were reported. A total of 352 preferred terms (PTs) and 24 system organ classes (SOCs) that were significantly disproportionally related to the four algorithms were included. The SOCs included blood and lymphatic system disorders (χ2 = 18,220.09, n = 4,325); skin and subcutaneous tissue disorders (χ2 = 408.06, n = 1,347); investigations (χ2 = 639.44, n = 3,925); musculoskeletal and connective tissue disorders (χ2 = 1,317.29, n = 588); and psychiatric disorders (χ2 = 1,098.47, n = 877). PT levels were screened for adverse drug reaction signals consistent with drug inserts, such as anemia, thrombocytopenia, liver function abnormalities, nausea and vomiting, as well as rarely reported adverse drug reactions, such as aplastic anemia, myelodysplastic syndromes, electrolyte disorders, cerebral edema, and high-frequency mutations.

Conclusion

The results of our investigation demonstrated both adverse effects that had been reported and a multitude of unreported adverse effects that were serious in nature and lacked a clear cause. These novel findings suggest that more attention should be given to the clinical conditions of patients after treatment to provide a more comprehensive perspective and understanding for further clarifying the safety of temozolomide.
Prevalence and risk factors for anxiety in patients with early- and middle-stage lung cancer: a cross-sectional study
Authors: Zhang Ruoqi , Zhang Peitong , Lin Yuejie , Guo Xiuwei , Wang Jing
Published: August 2024

Objective

Lung cancer is a leading cause of cancer-related morbidity and mortality worldwide, with patients frequently experiencing significant psychological distress, particularly anxiety. Despite the high prevalence of anxiety in patients with cancer, there is limited comprehensive research focusing on the specific factors influencing anxiety in patients with early- and middle-stage lung cancer within the context of Chinese medicine hospitals. Therefore, we aimed to investigate the epidemiology and factors influencing anxiety disorders in patients with early- and middle-stage primary bronchial lung cancer through a cross-sectional study.

Methods

A total of 340 patients with early and middle-stage lung cancer admitted to the outpatient ward of the oncology department at Guang’anmen Hospital from June 2023 to December 2023 were included in this study. Survey data, including the patients’ general condition questionnaire, Generalized Anxiety Scale (GAD-7), Hospital Anxiety and Depression Scale (HADS), and Mental Toughness Scale (CD-RISC-10), were collected and recorded in a database using a two-person input format. Data analysis was performed using SPSS 27.0 software.

Results

Out of the 340 patients with early- and middle-stage lung cancer included in this study, 133 had anxiety, resulting in an overall anxiety detection rate of 39.12%. The chi-square test showed that statistically significant differences in religion, marital status, surgical treatment, tobacco use, and alcohol history between the anxious and non-anxious groups (p < 0.05). Moreover, statistically significant differences were observed in per capita annual family income, pathological type, VAS score, targeted therapy, treatment stage, and mental toughness level (p < 0.001). Other factors were not significantly correlated with anxiety onset. Multivariate logistic regression analysis showed that higher per capita family income and completed treatment independently acted as protective factors against anxiety onset in patients with early- and middle-stage lung cancer. Conversely, rare pathological types, increased pain severity, and lower levels of mental toughness were identified as independent risk factors for anxiety onset in these patients.

Conclusion

Anxiety was prevalent in patients with early- and middle-stage lung cancers. Rare pathological types, increased pain severity, and lower levels of mental toughness were independent risk factors for anxiety. Therefore, clinicians and psychologists should pay more attention to patients with rare types of tumors, actively manage their pain symptoms, and consider implementing mental resilience training to improve patients’ mental toughness.
Research on China’s adolescent mental health policies — analysis based on PMC index model
Authors: Yang Chengning
Published: August 2024

Introduction

In recent years, the suicide rate among adolescents in China has shown a continuous upward trend, and mental health issues such as depression and excessive anxiety have become increasingly prominent. Awareness and concerns around poor mental health in adolescents is rising among the general public and in academic circles, but there is little research on adolescent mental health policies in China.

Methods

This article reviews the national policies on adolescent mental health from 2000 to 2023, and analyzes and evaluates the selected policy texts through the PMC index model.

Results

The study indicates growing national attention towards adolescent mental health policies in terms of both quantity and quality, with improved policy feasibility and synergy. However, shortcomings exist in the policy formulation process, including a lack of advocacy and supervision-oriented policies, a focus on short to medium-term effects, and inadequate comprehensive planning, hindering their swift implementation.

Discussion

In conclusion, facing the escalating crisis of adolescent mental health, the previous requirements of the education system are no longer sufficient. The government needs to further improve the top-down policy system, weave a safety net for mental health education and preventive intervention, and effectively promote the development of adolescent mental health.
Exploring the influence of Chinese online patient trust on telemedicine behavior: insights into perceived risk and behavior intention
Authors: Liu Chang , Wang Jiaqi , Chen Ran , Zhou Wusi
Published: August 2024

As a supplement to medical services, telemedicine is of great significance to alleviate the shortage of health resources in China. Based on the traditional consumer behavior measurement model the Technology Acceptance Mode/Theory of Planned Behavior (TAM/TPB), this paper divides online patient trust into six dimensions: perceived risk, personal trust tendency, doctors’ credibility, hospitals’ credibility, websites’ credibility, and system guarantee. On this basis, a structural equation model (SEM) was used to explore the influence of each dimension of online patient trust on online patient intention, behavior choice, and pre-factors. A total of 582 valid questionnaires were distributed to selected patients with experience in using mobile healthcare services in the vicinity of hospitals and communities, as well as to users who shared their experiences in the discussion forums of mobile healthcare websites. The results show that online patient trust has a significant positive impact on telemedicine behavior intention selection, with a standardized path coefficient being as high as 0.866. Doctors’ credibility, system guarantee, and website credibility have significant positive effects on online patient trust, with standardized path coefficients of 0.401, 0.260, and 0.226, respectively. Hospital trustworthiness and personal trust propensity have no significant effect on online patient trust. Perceived risk has a significant negative effect on online patient trust, with a standardized path coefficient of −0.118. The research findings suggest that health departments and mobile healthcare providers can enhance mobile healthcare services by considering the patients’ perspectives, elevate their online trust levels, and foster a deeper understanding, safety consciousness, and confidence in telehealth services. On this basis, it can be concluded that only the participation of government, medical subjects, and online patients can effectively reduce perceived risks, improve perceived characteristics of online patients, enhance online patient trust, and promote the real willingness and behavior choice for online medical services, effectively improving the positive role of telemedicine in increasing health benefits to people.
AttentionTTE: a deep learning model for estimated time of arrival
Authors: Li Mu , Feng Yijun , Wu Xiangdong
Published: August 2024

Estimating travel time (ETA) for arbitrary paths is crucial in urban intelligent transportation systems. Previous studies primarily focus on constructing complex feature systems for individual road segments or sub-segments, which fail to effectively model the influence of each road segment on others. To address this issue, we propose an end-to-end model, AttentionTTE. It utilizes a self-attention mechanism to capture global spatial correlations and a recurrent neural network to capture temporal dependencies from local spatial correlations. Additionally, a multi-task learning module integrates global spatial correlations and temporal dependencies to estimate the travel time for both the entire path and each local path. We evaluate our model on a large trajectory dataset, and extensive experimental results demonstrate that AttentionTTE achieves state-of-the-art performance compared to other methods.
The prevalence of attention-deficit hyperactivity disorder and its associated factors among children in Ethiopia, 2024: a systematic review and meta-analysis
Authors: Azmeraw Molla , Temesgen Dessie , Kassaw Amare , Zemariam Alemu Birara , Kerebeh Gashaw , Abebe Gebremeskel Kibret , Alamaw Addis Wondmagegn , Abate Biruk Beletew
Published: August 2024

Introduction

Attention-deficit hyperactivity disorder (ADHD) is a neuropsychiatric condition that affects children. Its magnitude varies by area, ranging from 0.2% to 26.8%. Even though there is debate, culture and geographical location may have little or no influence on the epidemiology of ADHD worldwide. Despite this variation, debate over the national prevalence and location of ADHD is unknown in Ethiopia. Therefore, this study aimed to assess the pooled prevalence of ADHD and its contributing factors among children.

Methods

Electronic databases, including Google Scholar, PubMed, Scopus, EMBASE, Web of Science, ScienceDirect, and institutional repositories, were searched. The studies that covered the prevalence and/or risk factors of ADHD in children were included in the collection. The Joanna Briggs Institute quality rating tool was used to rate the quality of each study. The data were extracted using Microsoft Excel 2019, and the statistical analysis was performed using STATA 17.0. Using a random-effects model, we evaluated the combined prevalence of ADHD and associated factors. The Cochrane Q-test and I2 test statistics were used to quantify the heterogeneity. Furthermore, publication bias was examined using funnel plot graphs and Egger's tests. A Galbraith plot was employed to illustrate outliers. Sensitivity analysis was also applied.

Result

This study included a total of six articles with 4,338 participants. The pooled prevalence estimate of ADHD was 8.81% [95% confidence interval (CI), 4.52–13.11; I2 = 96.95%; P = 0.001]. Age 6–12 [adjusted odds ratio (AOR) = 3.51 (95% CI, 1.38–5.64), I2 = 0%; P = 0.001], being male [AOR = 1.94 (95% CI, 1.09–2.79), I2 = 0%; P = 0.001], and living with a single parent [AOR = 4.92 (95% CI, 1.24–861), I2 = 0%; P = 0.001] were significant variables.

Conclusion and recommendation

One out of every 12 children in Ethiopia suffers from ADHD. Living with a single parent, being male, and being between the ages of 6 and 12 were risk factors for ADHD. A nationwide study with a large sample size may be required to ascertain the true impact of ADHD. It may be crucial to improve school health services to identify ADHD early and lessen its long-term effects.
No-Tillering System: Is It Worth Conducting Soil Scarification as an Option for “Field Reform”?
Authors: de Carvalho Machione Eduardo , de Queiroz Renata Fernandes , Lopes Afonso , Furlani Carlos Eduardo Angeli , Canata Tatiana Fernanda
Published: August 2024

The adoption of a no-till system presents difficulties in maintaining the potential crop yield under long-term conditions when compacted soil layers are observed in the field. The research objective was to assess the benefits of soil intervention in a traditional area of no-till system while considering the production parameters of corn (Zea mays L.) under tropical conditions. The area was installed considering 24 experimental units, including 6 treatments, a 3 × 2 scheme, and 4 repetitions (3 soil management systems and 2 crop hybrids). The soil scarification was carried out at two depths, 0.15 m and 0.30 m, to compare its influence on corn yield. The number of days of plants’ emergence, seed depth, longitudinal distribution of seedlings, phytotechnical parameters, and crop yield in the field were evaluated. The dataset was subjected to the F Test and Tukey test (p < 0.05) to compare the means by each attribute and treatment. The adopted hybrids, as well as soil-management systems in the experimental area, did not interfere with the quality of the sowing and quantitative parameters of the crop (CV = 15% for normal distribution on plants’ spacing within rows). So, there is no need to scarify soil with the aim of increasing corn yield under the evaluated field conditions.
Fatigue Analysis of PTO Gearboxes in Paddy Power Chassis Using Measured Loads
Authors: He Jianfei , Wang Zaiman , Gao Bo , Yu Dongyang , Ma Yifan , Zhong Wenneng , Zeng Zhihao , Guo Ziyou , Wang Jun
Published: August 2024

This study aims to analyze the fatigue life of a PTO (power take-off) gearbox used in a paddy field power chassis. The analysis considers factors such as stress concentration, dimensions, surface quality, and load characteristics affecting fatigue life. A finite element simulation was conducted using the Ansys 2022 software to identify the critical point of the PTO shell. The modified nominal stress fatigue analysis method, incorporating a stress adjustment coefficient, was employed to derive the modified S-N curve. Combined with the measured load data of the PTO bench operation, the load data and the 3D model of the PTO shell were imported into the fatigue analysis software n-code to analyze the fatigue life of the PTO gearbox of a paddy field power chassis and compare it with the prediction results from the traditional stress field strength method. The findings indicate that the optimized stress adjustment coefficient method predicts a fatigue life (31,699 h) closer to the actual operational life (20,000 h) compared to the traditional method (39,151 h). This research contributes to the advancement of the analytical techniques for predicting fatigue life in critical components of agricultural machinery.
The Influence of the Hybrid Compound Nd(NO3)3@Zn-MOF on the Growth of Vanilla (Vanilla planifolia Jacks. ex Andrews) Cultured In Vitro: A Preliminary Study
Authors: Cruz-Cruz Carlos Alberto , De Jesús García-Zárate Xóchitl , Spinoso-Castillo José Luis , Peña-Rodríguez Rodolfo , Colorado-Peralta Raúl , Sánchez-Páez Ricardo , Bello-Bello Jericó Jabín
Published: August 2024

Hybrid compounds have a significant impact on agriculture as slow macro- and micronutrient administration systems. This study aimed to evaluate the synthesis and effect of the hybrid compound Nd(NO3)3@Zn-MOF in different concentrations on the in vitro growth of vanilla (Vanilla planifolia Jacks. ex Andrews). A total of 13 vanilla plantlets per treatment were cultivated in test tubes with semi-solid Murashige and Skoog (MS) medium and without growth regulators and treated with 0, 5, 10, 15, and 30 mg L−1 of Nd(NO3)3@Zn-MOF. After 60 days of culture, we evaluated different morphological and biochemical parameters, such as shoot length, root length, the number of roots, the number of leaves, total chlorophyll and carotenoid content, antioxidant capacity, and phenolic compound content. Our results showed that the Nd(NO3)3@Zn-MOF at 10 mg L−1 concentration increased plantlet length. Furthermore, we observed an increase in root length and number with the 5 and 10 mg L−1 concentrations, and a decrease in these same parameters with the 15 and 30 mg L−1 Nd(NO3)3@Zn-MOF concentrations. There were no significant differences regarding the number of leaves or total chlorophyll content. As for the antioxidant capacity, we observed an increase with 5, 10, and 15 mg L−1 of Nd(NO3)3@Zn-MOF and a decrease with the highest concentration. Finally, the phenolic and carotenoid content decreased with the 15 and 30 mg L−1 Nd(NO3)3@Zn-MOF concentrations compared to the control. In conclusion, the hybrid compound Nd(NO3)3@Zn-MOF showed beneficial effects on the growth, physiology, and biochemistry of V. planifolia in vitro when plants were treated at low concentrations. Additionally, the high concentrations used in this study did not induce toxicity. Our findings suggest that Nd(NO3)3@Zn-MOF could be used as a biostimulant in vanilla during its in vitro culture. However, due to the hormetic effect and the possible different reactions of different genotypes, this requires further detailed research.
Dynamic Changes and Influencing Factors Analysis of Groundwater Icings in the Permafrost Region in Central Sakha (Yakutia) Republic under Modern Climatic Conditions
Authors: Yu Miao , Pavlova Nadezhda , Zhao Jing , Dai Changlei
Published: August 2024

In central Sakha (Yakutia) Republic, groundwater icings, primarily formed by intrapermafrost water, are less prone to contamination and serve as a stable freshwater resource. The periodic growth of icings threatens infrastructure such as roads, railways, and bridges in permafrost areas. Therefore, research in this field has become urgently necessary. This study aims to analyze the impacts of various factors on the scale of icing formation using Landsat satellite data, Gravity Recovery and Climate Experiment (GRACE)/GRACE Follow-On (GRACE-FO) data, Global Land Data Assimilation System (GLDAS) data, and field observation results. The results showed that the surface area of icings in the study area showed an overall increasing trend from 2002 to 2022, with an average growth rate of 0.06 km2/year. Suprapermafrost water and intrapermafrost water are the main sources of icings in the study area. The total Groundwater Storage Anomaly (GWSA) values from October to April showed a strong correlation with the maximum icing areas. Icings fed by suprapermafrost water were influenced by precipitation in early autumn, while those fed by intrapermafrost water were more affected by talik size and distribution. Climate warming contributed to the degradation of the continuous permafrost covering an area of 166 km2 to discontinuous permafrost, releasing additional groundwater. This may also be one of the reasons for the observed increasing trend in icing areas. This study can provide valuable insights into water resource management and infrastructure construction in permafrost regions.