bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Vibrotherapy on Body Fatness, Blood Parameters and Fibrinogen Concentration in Elderly Men
Authors: Kabata-Piżuch Anna , Suder Agnieszka , Jagielski Paweł , Kubasiak Katarzyna , Handzlik Paulina , Teległów Aneta , Marchewka Anna
Published: July 2021

Elderly people need activities that will positively contribute to a satisfactory process of getting older. Vibration training uses mechanical stimulus of a vibrational character that, similarly to other forms of physical activity, affects metabolic processes and conditions of health. The aim of this work was to assess the influence of thirty vibration treatments on body fatness, hematologic and rheologic indexes of blood, and proteinogram and fibrinogen concentration in elderly men’s blood. The study included twenty-one males, aged 60–70 years (mean age 65.3 ± 2.7), who were randomly assigned into a vibrotherapy group (VG) and took part in interventions on mattresses generating oscillatory-cycloid vibrations, and a control group (CG), without interventions. In all patients the following assessments were performed twice: an assessment of body fatness using the bioimpedance method, a complete blood count with a hematology analyzer, and erythrocyte aggregation by a laser-optical rotational cell analyzer; whereas, total plasma protein and fibrinogen values were established, respectively, by biuret and spectrophotometric methods. In order to compare the impact of vibrotherapy on changes in the analyzed variables, analysis of variance (ANOVA) or the Wilcoxon test were used. After applying thirty vibration treatments in the VG, a significant decrease in body fatness parameters was confirmed: BM (∆BM: −2.7 ± 2.0; p = 0.002), BMI (∆BMI: −0.9 ± 0.7; p = 0.002), BF (∆BF: −2.5 ± 2.5; p = 0.013), and %BF (∆%BF: −2.0 ± 2.7; p = 0.041), as well as in RBC (∆RBC: −0.1 ± 0.1; p = 0.035). However, changes in erythrocyte aggregation and proteinogram were not confirmed. It was found that after thirty treatments with VG, a significant decrease of fibrinogen level took place (∆ = −0.3 ± 0.3, p = 0.005). Application of thirty vibrotherapy treatments positively affected body fatness parameters and fibrinogen concentrations in the examined. However, further research should include a greater number of participants.
Management of Corneal Clouding in Patients with Mucopolysaccharidosis
Authors: McGrath Orlaith , Au Leon , Ashworth Jane
Published: July 2021

Mucopolysaccharidoses (MPS) are a rare group of lysosomal storage disorders characterized by the accumulation of incompletely degraded glycosaminoglycans (GAGs) in multiple organ systems including the eye. Visual loss occurs in MPS predominantly due to corneal clouding and retinopathy, but the sclera, trabecular meshwork and optic nerve may all be affected. Despite the success of therapies such as enzyme replacement therapy (ERT) and hematopoietic stem-cell transplantation (HSCT) in improving many of the systemic manifestations of MPS, their effect on corneal clouding is minimal. The only current definitive treatment for corneal clouding is corneal transplantation, usually in the form of a penetrating keratoplasty or a deep anterior lamellar keratoplasty. This article aims to provide an overview of corneal clouding, its current clinical and surgical management, and significant research progress.
Resting Heart Rate and Cardiovascular Outcomes during Intensive and Standard Blood Pressure Reduction: An Analysis from SPRINT Trial
Authors: Sobieraj Piotr , Siński Maciej , Lewandowski Jacek
Published: July 2021

The association between elevated resting heart rate (RHR) as a cardiovascular risk factor and lowering of systolic blood pressure (SBP) to currently recommended values remain unknown. Systolic Blood Pressure Intervention Trial (SPRINT) data obtained from the NHLBI were used to describe the relationship between RHR and SBP reduction to <120 mmHg compared to SBP reduction to <140 mmHg. The composite clinical endpoint (CE) was defined as myocardial infarction, acute coronary syndrome, decompensation of heart failure, stroke, or cardiovascular death. Increased RHR was associated with a higher CE risk compared with low RHR in both treatment arms. A more potent increase of risk for CE was observed in subjects who were allocated to the SBP < 120 mmHg treatment goal. A similar effect of intensive and standard blood pressure (BP) reduction (p for interaction, 0.826) was observed in subjects with RHR in the 5th quintile (hazard ratio, 0.78, with 95% confidence interval (CI), 0.55–1.11) and in other quintiles of baseline RHR (hazard ratio, 0.75, with 95% CI, 0.62–0.90). Lower in-trial than baseline RHR was associated with reduced CE risk (hazard ratio, 0.80, with 95% CI, 0.66–0.98). We concluded that elevated RHR remains an essential risk factor independent of SBP reduction.
Association between Objectively Measured Physical Activity and Arterial Stiffness in Children with Congenital Heart Disease
Authors: Willinger Laura , Brudy Leon , Oberhoffer-Fritz Renate , Ewert Peter , Müller Jan
Published: July 2021

Background: The association between physical activity (PA) and arterial stiffness is particularly important in children with congenital heart disease (CHD) who are at risk for arterial stiffening. The aim of this study was to examine the association between objectively measured PA and arterial stiffness in children and adolescents with CHD. Methods: In 387 children and adolescents with various CHD (12.2 ± 3.3 years; 162 girls) moderate-to-vigorous PA (MVPA) was assessed with the “Garmin vivofit jr.” for 7 consecutive days. Arterial stiffness parameters including pulse wave velocity (PWV) and central systolic blood pressure (cSBP) were non-invasively assessed by oscillometric measurement via Mobil-O-Graph®. Results: MVPA was not associated with PWV (ß = −0.025, p = 0.446) and cSBP (ß = −0.020, p = 0.552) in children with CHD after adjusting for age, sex, BMI z-score, peripheral systolic blood pressure, heart rate and hypertensive agents. Children with CHD were remarkably active with 80% of the study population reaching the WHO recommendation of average 60 min of MVPA per day. Arterial stiffness did not differ between low-active and high-active CHD group after adjusting for age, sex, BMI z-score, peripheral systolic blood pressure, heart rate and hypertensive agents (PWV: F = 0.530, p = 0.467; cSBP: F = 0.843, p = 0.359). Conclusion: In this active cohort, no association between PA and arterial stiffness was found. Longer exposure to the respective risk factors of physical inactivity might be necessary to determine an impact of PA on the vascular system.
A Possible Role of Remdesivir and Plasma Therapy in the Selective Sweep and Emergence of New SARS-CoV-2 Variants
Authors: Colson Philippe , Devaux Christian A. , Lagier Jean-Christophe , Gautret Philippe , Raoult Didier
Published: July 2021

Since summer 2020, SARS-CoV-2 strains at the origin of the COVID-19 pandemic have suddenly been replaced by new SARS-CoV-2 variants, some of which are highly transmissible and spread at a high rate. These variants include the Marseille-4 lineage (Nextclade 20A.EU2) in Europe, the 20I/501Y.V1 variant first detected in the UK, the 20H/501Y.V2 variant first detected in South Africa, and the 20J/501Y.V3 variant first detected in Brazil. These variants are characterized by multiple mutations in the viral spike protein that is targeted by neutralizing antibodies elicited in response to infection or vaccine immunization. The usual coronavirus mutation rate through genetic drift alone cannot account for such rapid changes. Recent reports of the occurrence of such mutations in immunocompromised patients who received remdesivir and/or convalescent plasma or monoclonal antibodies to treat prolonged SARS-CoV-2 infections led us to hypothesize that experimental therapies that fail to cure the patients from COVID-19 could favor the emergence of immune escape SARS-CoV-2 variants. We review here the data that support this hypothesis and urge physicians and clinical trial promoters to systematically monitor viral mutations by whole-genome sequencing for patients who are administered these treatments.
Transcutaneous Electrical Neuromodulation of the Cervical Spinal Cord Depends Both on the Stimulation Intensity and the Degree of Voluntary Activity for Training. A Pilot Study
Authors: Kumru Hatice , Rodríguez-Cañón María , Edgerton Victor R. , García Loreto , Flores África , Soriano Ignasi , Opisso Eloy , Gerasimenko Yury , Navarro Xavier , García-Alías Guillermo , Vidal Joan
Published: July 2021

Electrical enabling motor control (eEmc) through transcutaneous spinal cord stimulation offers promise in improving hand function. However, it is still unknown which stimulus intensity or which muscle force level could be better for this improvement. Nine healthy individuals received the following interventions: (i) eEmc intensities at 80%, 90% and 110% of abductor pollicis brevis motor threshold combined with hand training consisting in 100% handgrip strength; (ii) hand training consisting in 100% and 50% of maximal handgrip strength combined with 90% eEmc intensity. The evaluations included box and blocks test (BBT), maximal voluntary contraction (MVC), F wave persistency, F/M ratio, spinal and cortical motor evoked potentials (MEP), recruitment curves of spinal MEP and cortical MEP and short-interval intracortical inhibition. The results showed that: (i) 90% eEmc intensity increased BBT, MVC, F wave persistency, F/M ratio and cortical MEP recruitment curve; 110% eEmc intensity increased BBT, F wave persistency and cortical MEP and recruitment curve of cortical MEP; (ii) 100% handgrip strength training significantly modulated MVC, F wave persistency, F/M wave and cortical MEP recruitment curve in comparison to 50% handgrip strength. In conclusion, eEmc intensity and muscle strength during training both influence the results for neuromodulation at the cervical level.
Evidence and Applicability of Stress Cardiovascular Magnetic Resonance in Detecting Coronary Artery Disease: State of the Art
Authors: Baritussio Anna , Scatteia Alessandra , Dellegrottaglie Santo , Bucciarelli-Ducci Chiara
Published: July 2021

Cardiovascular magnetic resonance is increasingly used in clinical practice, as it has emerged over the years as an invaluable imaging technique for diagnosis and prognosis, with clear-cut applications in managing patients with both ischemic and non-ischemic heart disease. In this review, we focus on the evidence and clinical application of stress CMR in coronary artery disease from diagnosis to prognosis.
Effectiveness and Safety of Pangenotypic Regimens in the Most Difficult to Treat Population of Genotype 3 HCV Infected Cirrhotics
Authors: Zarębska-Michaluk Dorota , Jaroszewicz Jerzy , Parfieniuk-Kowerda Anna , Janczewska Ewa , Dybowska Dorota , Pawłowska Małgorzata , Halota Waldemar , Mazur Włodzimierz , Lorenc Beata , Janocha-Litwin Justyna , Simon Krzysztof , Piekarska Anna , Berak Hanna , Klapaczyński Jakub , Stępień Piotr , Sobala-Szczygieł Barbara , Citko Jolanta , Socha Łukasz , Tudrujek-Zdunek Magdalena , Tomasiewicz Krzysztof , Sitko Marek , Dobracka Beata , Krygier Rafał , Białkowska-Warzecha Jolanta , Laurans Łukasz , Flisiak Robert
Published: July 2021

There is still limited data available from real-world experience studies on the pangenotypic regimens in patients with genotype (GT) 3 hepatitis C virus (HCV) infection and liver cirrhosis. The current study aimed to evaluate the efficacy and safety of pangenotypic regimens in this difficult-to-treat population. A total of 236 patients with mean age 52.3 ± 11.3 years and male predominance (72%) selected from EpiTer-2 database were included in the analysis; 72% of them were treatment-naïve. The majority of patients (55%) received the combination of sofosbuvir/velpatasvir (SOF/VEL), 71 without and 58 with ribavirin (RBV), whereas the remaining 107 individuals were assigned to glecaprevir/pibrentasvir (GLE/PIB). The effectiveness of the treatment following GLE/PIB and SOF/VEL regimens (96% and 93%) was higher compared to SOF/VEL + RBV option (79%). The univariate analysis demonstrated the significantly lower sustained virologic response in males, in patients with baseline HCV RNA ≥ 1,000,000 IU/mL, and among those who failed previous DAA-based therapy. The multivariate logistic regression analysis recognized only the male gender and presence of ascites at baseline as the independent factors of non-response to treatment. It should be emphasized that despite the availability of pangenotypic, strong therapeutic options, GT3 infected patients with cirrhosis still remain difficult-to-treat, especially those with hepatic impairment and DAA-experienced.
Electric Scooter-Related Injuries: A New Epidemic in Orthopedics
Authors: Coelho Alexandre , Feito Pablo , Corominas Laura , Sánchez-Soler Juan Francisco , Pérez-Prieto Daniel , Martínez-Diaz Santos , Alier Albert , Monllau Joan Carles
Published: July 2021

Background: The use of electric scooters has increased considerably as they are an accessible means of transportation. The number of injuries from falls and collisions has risen significantly. Therefore, the aim of the study was to describe demographics injury patterns of electric scooter accidents produced over one year. Methods: A prospective observational study of electric scooter- related injuries presented in the emergency room from May 2019 until May 2020. The inclusion criteria was based on the direct cause of injury produced while an electric scooter was in use. Demographic data, the use of a helmet or the lack thereof, accident mechanism, injury time, type of injury produced, and the treatment applied were collected. Results: Over the study period, 397 patients were identified with a total of 422 injuries. The mean age was 30.8 years, with 12.6% of patients being minors. The patients mainly presented in evening hours and in summertime at the emergency department. Of the total injuries seen, 46.9% were fractures. Some 25% of the total cases required surgery. Only 19% of the riders wore a helmet at the time of the accident. Most of the fractures were to the upper limbs (62.6%). There was a greater incidence of radius fractures. Conclusion: Injuries incurred while using electric scooters are an emerging phenomenon, despite existing regulations. In this study, most injuries occurred in young men and were due to falls from the vehicle. Nearly half of those injuries were fractures to the upper limbs. Surprisingly, 50% of the fractures required surgery.
The Framingham Risk Score Is Associated with Chronic Graft Failure in Renal Transplant Recipients
Authors: Anderson Josephine L. C. , Poot Margot L. , Steffen Hannah L. M. , Kremer Daan , Bakker Stephan J. L. , Tietge Uwe J. F.
Published: July 2021

Predicting chronic graft failure in renal transplant recipients (RTR) is an unmet clinical need. Chronic graft failure is often accompanied by transplant vasculopathy, the formation of de novo atherosclerosis in the transplanted kidney. Therefore, we determined whether the 10-year Framingham risk score (FRS), an established atherosclerotic cardiovascular disease prediction module, is associated with chronic graft failure in RTR. In this prospective longitudinal study, 600 well-characterised RTR were followed for 10 years. The association with death-censored chronic graft failure (n = 81, 13.5%) was computed. An extended Cox model showed that each one percent increase of the FRS significantly increased the risk of chronic graft failure by 4% (HR: 1.04, p < 0.001). This association remained significant after adjustment for potential confounders, including eGFR (HR: 1.03, p = 0.014). Adding the FRS to eGFR resulted in a higher AUC in a receiver operating curve (AUC = 0.79, p < 0.001) than eGFR alone (AUC = 0.75, p < 0.001), and an improvement in the model likelihood ratio statistic (67.60 to 88.39, p < 0.001). These results suggest that a combination of the FRS and eGFR improves risk prediction. The easy to determine and widely available FRS has clinical potential to predict chronic graft failure in RTR.
Sex-Specific Signature in the Circulating NLRP3 Levels of Saudi Adults with Metabolic Syndrome
Authors: Al-Daghri Nasser M. , Wani Kaiser , AlHarthi Hind , Alghamdi Amani , Alnaami Abdullah M. , Yakout Sobhy M.
Published: July 2021

Recently, inflammasomes such as NLRP3 as cytosolic pattern-recognition receptors have been implicated in the development of inflammation; however, limited investigations report the circulating levels of this protein. The objective, thus, was to investigative circulating NLRP3 levels in Saudi patients with a low-grade inflammatory disorder called metabolic syndrome (MetS). Two hundred Saudi adults aged 30–65, with or without MetS diagnosed on the basis of National Cholesterol Education Programme Adult Treatment Panel III (NCEP ATP III) criteria, were randomly recruited. Five MetS components were established according to the diagnostic criteria in the study subjects. Circulating levels of NLRP3 and known inflammation markers, such as tumor necrosis factor α (TNF-α), C-reactive protein (CRP) and interleukins (IL-1β and IL-18), were measured in the blood samples taken from the study subjects. Gender-based analysis showed a significant elevated circulating levels of NLRP3 in non-MetS men compared to non-MetS females (p < 0.001). Moreover, an increase in circulating levels of NLRP3 with a number of MetS components (p = 0.038) was observed only in females. A significant positive correlation of NLRP3 levels with age (r = 0.20, p = 0.04), BMI (r = 0.32, p < 0.01) and waist (r = 0.24, p = 0.02) and a significant negative correlation between NLRP3 and HDL-cholesterol (r= −0.21, p = 0.03) were also observed in females. Logistic regression analysis also yielded a sex-specific positive association of NLRP3 with MetS in females, with this association influenced mostly by central obesity and dyslipidemia components of MetS. In conclusion, this study suggests a sexual disparity in the circulating levels of NLRP3, with a trend of increasing circulating NLRP3 levels with increasing MetS components observed only in females, influenced mostly by adiposity and dyslipidemia components of MetS. Longitudinal studies with a larger sample size and investigating sex-specific hormones with NLRP3 would be needed to establish a causal relationship of NLRP3 with MetS.
Spontaneously Reported Adverse Drug Reactions and Their Description in Hospital Discharge Reports: A Retrospective Study
Authors: Aguilera Cristina , Agustí Antònia , Pérez Eulàlia , Gracia Rosa M. , Diogène Eduard , Danés Immaculada
Published: July 2021

The inclusion of spontaneously reported adverse drug reactions (ADRs) in hospital discharge reports was examined, in addition to the factors associated with their inclusion, the resulting therapeutic decisions, and any recommendations made upon patient discharge regarding the suspected offending drugs. ADRs that were spontaneously reported during 2017 and 2018 to the pharmacovigilance program were retrospectively analyzed. Information regarding patient characteristics, drug treatments, and ADRs was collected from the ADR notifications and from patient electronic medical records. The dependent variable was the mentioning of ADRs in the discharge reports, while characteristics of the ADRs, pharmacovigilance causality algorithms, and some of the suspected drugs themselves were the independent variables during bivariant analysis. A total of 286 reports of suspected ADRs from 271 patients (50.2% female; 77% adults) were included. Information regarding the ADRs was present in the discharge reports for 238 reports (83.2%); the ADR seriousness and the lack of potential alternative causes were the only associated factors. Withdrawal or withdrawal and substitution by an alternative drug were the most common therapeutic decisions, although often no recommendation was made. Overall, there is still room for improvement in terms of including information related to ADRs in hospital discharge reports.
Long-Term Safety in HBsAg-Negative, HBcAb-Positive Patients with Rheumatic Diseases Receiving Maintained Steroid Therapy after Pulse Therapy
Authors: Lin Ying-Cheng , Chen Yen-Ju , Lee Shou-Wu , Lee Teng-Yu , Chen Yi-Hsing , Huang Wen-Nan , Yang Sheng-Shun , Chen Yi-Ming
Published: July 2021

The risk of hepatitis B virus (HBV) reactivation in hepatitis B surface antigen (HBsAg)-negative, antibody to hepatitis B core antigen (anti-HBc)-positive patients after glucocorticoid (GC) pulse therapy remains unclear. Aims: Our study aimed to examine the safety of GC pulse therapy in HBsAg-negative, anti-HBc-positive rheumatic patients. Methods: Medical records of HBsAg-negative, anti-HBc-positive patients receiving GC pulse therapy to treat rheumatic diseases were reviewed. The primary outcome was HBV-associated hepatitis occurring within the first year after GC pulse therapy; the secondary outcome was HBsAg seroreversion occurring during the follow-up period. Results: We identified 5222 HBsAg-negative, anti-HBc-positive patients with rheumatic diseases who had attended Taichung Veterans General Hospital from October 2006 to December 2018. A total of 689 patients had received GC pulse therapy, with 424 patients being analyzed. Hepatitis was noted in 28 patients (6.6%) within the first year after GC pulse therapy, but none had been diagnosed as HBV-associated hepatitis. Three patients (0.7%) later developed HBsAg seroreversion, with a median interval of 97 months from the first episode of GC pulse therapy. These cases concurrently had maintained high dose oral prednisolone (≥20 mg prednisolone daily for over 4 weeks). Conclusions: Amongst the HBsAg-negative, anti-HBc-positive rheumatic patients treated with GC pulse therapy, the risk of HBV-associated hepatitis within the first year was low. HBsAg seroreversion may have developed in the later stage, but only in those patients who had maintained high-dose oral steroid.
Regulation of the Brain Neural Niche by Soluble Molecule Akhirin
Authors: Kudo Mikiko , Ohta Kunimasa
Published: July 2021

In the central nervous system (CNS), which comprises the eyes, spinal cord, and brain, neural cells are produced by the repeated division of neural stem cells (NSCs) during the development of the CNS. Contrary to the notion that the CNS is relatively static with a limited cell turnover, cells with stem cell-like properties have been isolated from most neural tissues. The microenvironment, also known as the NSC niche, consists of NSCs/neural progenitor cells, other neurons, glial cells, and blood vessels; this niche is thought to regulate neurogenesis and the differentiation of NSCs into neurons and glia. Although it has been established that neurons, glia, and blood vessels interact with each other in a complex manner to generate neural tissues in the NSC niche, the underlying molecular mechanisms in the CNS niche are unclear. Herein, we would like to introduce the extracellular secreted protein, Akhirin (AKH; Akhi is the Bengali translation for eye). AKH is specifically expressed in the CNS niche—the ciliary body epithelium in the retina, the central canal of the spinal cord, the subventricular zone, and the subgranular zone of the dentate gyrus of the hippocampus—and is supposedly involved in NSC niche regulation. In this review, we discuss the role of AKH as a niche molecule during mouse brain formation.
Why Would You Choose to Do an Extreme Sport?
Authors: Musumeci Giuseppe
Published: July 2021

Why do so many athletes keep practicing extreme sports, even though they know the danger of risking their lives [...]
The Constantly Evolving Role of Medical Image Processing in Oncology: From Traditional Medical Image Processing to Imaging Biomarkers and Radiomics
Authors: Marias Kostas
Published: July 2021

The role of medical image computing in oncology is growing stronger, not least due to the unprecedented advancement of computational AI techniques, providing a technological bridge between radiology and oncology, which could significantly accelerate the advancement of precision medicine throughout the cancer care continuum. Medical image processing has been an active field of research for more than three decades, focusing initially on traditional image analysis tasks such as registration segmentation, fusion, and contrast optimization. However, with the advancement of model-based medical image processing, the field of imaging biomarker discovery has focused on transforming functional imaging data into meaningful biomarkers that are able to provide insight into a tumor’s pathophysiology. More recently, the advancement of high-performance computing, in conjunction with the availability of large medical imaging datasets, has enabled the deployment of sophisticated machine learning techniques in the context of radiomics and deep learning modeling. This paper reviews and discusses the evolving role of image analysis and processing through the lens of the abovementioned developments, which hold promise for accelerating precision oncology, in the sense of improved diagnosis, prognosis, and treatment planning of cancer.
On the Limitations of Visual-Semantic Embedding Networks for Image-to-Text Information Retrieval
Authors: Gong Yan , Cosma Georgina , Fang Hui
Published: July 2021

Visual-semantic embedding (VSE) networks create joint image–text representations to map images and texts in a shared embedding space to enable various information retrieval-related tasks, such as image–text retrieval, image captioning, and visual question answering. The most recent state-of-the-art VSE-based networks are: VSE++, SCAN, VSRN, and UNITER. This study evaluates the performance of those VSE networks for the task of image-to-text retrieval and identifies and analyses their strengths and limitations to guide future research on the topic. The experimental results on Flickr30K revealed that the pre-trained network, UNITER, achieved 61.5% on average Recall@5 for the task of retrieving all relevant descriptions. The traditional networks, VSRN, SCAN, and VSE++, achieved 50.3%, 47.1%, and 29.4% on average Recall@5, respectively, for the same task. An additional analysis was performed on image–text pairs from the top 25 worst-performing classes using a subset of the Flickr30K-based dataset to identify the limitations of the performance of the best-performing models, VSRN and UNITER. These limitations are discussed from the perspective of image scenes, image objects, image semantics, and basic functions of neural networks. This paper discusses the strengths and limitations of VSE networks to guide further research into the topic of using VSE networks for cross-modal information retrieval tasks.
Effect of Summer Typhoon Linfa on the Chlorophyll-a Concentration in the Continental Shelf Region of Northern South China Sea
Authors: Wang Tongyu , Zhang Shuwen
Published: July 2021

Based on both physical and biological data collected from multi-source satellite during summer typhoon Linfa, we found that the typhoon triggered two phytoplankton declines and three phytoplankton blooms in the northern South China Sea (SCS), where the waters were influenced by coastal upwelling and the input of terrigenous materials from the Pearl River estuary (PRE). One phytoplankton decline (about a 3-fold reduction) in the continental shelf region can probably be attribute to the limited nutrient supply induced by the decayed coastal northeastern current and onshore Ekman transport (OET) and Kuroshio intrusion water, as well as the uplifted subsurface’s low chlorophyll-a (Chl-a) concentration driven by vertical mixing and upwelling. Another phytoplankton decline (about a 3.5-fold reduction) in the eastern Leizhou Peninsula-coastal upwelling region is probably caused by OET and a decayed coastal northern current. Conversely, the decayed coastal current, OET, and the vertical mixing and upwelling could lead to the transport of nutrient-rich water from the PRE to the nearshore region of the southwestern PRE mouth, and from the subsurface layer to the surface, respectively, thereby stimulating the growth of phytoplankton in the nearshore region (increased by about 4-fold) and the open ocean (increased by about 2.3-fold). In the Shantou (the coastal upwelling region), the phytoplankton responses to nutrient supply were feeble when phytoplankton was already growing in nutrient replete conditions. In addition, the OET and the high turbidity barely resulted in moderate phytoplankton bloom (increased by 38%). In summary, the physical driving forces associated with typhoons that modulates phytoplankton dynamics are the nutrient and phytoplankton transportation in the northern SCS during the wet season.
Machine Learning Based Moored Ship Movement Prediction
Authors: Alvarellos Alberto , Figuero Andrés , Carro Humberto , Costas Raquel , Sande José , Guerra Andrés , Peña Enrique , Rabuñal Juan
Published: July 2021

Several port authorities are involved in the R+D+i projects for developing port management decision-making tools. We recorded the movements of 46 ships in the Outer Port of Punta Langosteira (A Coruña, Spain) from 2015 until 2020. Using this data, we created neural networks and gradient boosting models that predict the six degrees of freedom of a moored vessel from ocean-meteorological data and ship characteristics. The best models achieve, for the surge, sway, heave, roll, pitch and yaw movements, a 0.99, 0.99, 0.95, 0.99, 0.98 and 0.98 R2 in training and have a 0.10 m, 0.11 m, 0.09 m, 0.9°, 0.11° and 0.15° RMSE in testing, all below 10% of the corresponding movement range. Using these models with forecast data for the weather conditions and sea state and the ship characteristics and berthing location, we can predict the ship movements several days in advance. These results are good enough to reliably compare the models’ predictions with the limiting motion criteria for safe working conditions of ship (un) loading operations, helping us decide the best location for operation and when to stop operations more precisely, thus minimizing the economic impact of cargo ships unable to operate.
Investigation of the Spatio-Temporal Behaviour of Submarine Groundwater Discharge Using a Low-Cost Multi-Sensor-Platform
Authors: Tholen Christoph , Parnum Iain , Rofallski Robin , Nolle Lars , Zielinski Oliver
Published: July 2021

Submarine groundwater discharge (SGD) is an important pathway of nutrients into coastal areas. During the last decades, interest of researchers in SGDs has grown continuously. However, methods applied for SGD research usually focus on the aquifer or on the mixing processes on larger scales. The distribution of discharged water within the water column is not well investigated. Small remotely operated vehicles (ROV) equipped with environmental sensors can be used to investigate the spatial distribution of environmental parameters in the water column. Herein, a low-cost multi-sensor platform designed to investigate the spatial distribution of water quality properties is presented. The platform is based on an off-the-shelf underwater vehicle carrying various environmental sensors and a short-baseline localisation system. This contribution presents the results of SGD investigations in the area of Woodman Point (Western Australia). Various potential SGD plumes were detected using a skiff equipped with a recreational echo sounder. It was demonstrated that this inexpensive equipment could be used to detect and investigate SGDs in coastal areas. In addition, the low-cost multi-sensor platform was deployed to investigate the spatial distribution of environmental parameters including temperature (T), electric conductivity (EC), dissolved oxygen (DO), oxidation-reduction potential (ORP), pH, and dissolved organic matter fluorescence (FDOM). Three ROV surveys were conducted from different skiff locations. Analyses of the spatial distribution of the environmental parameters allowed the identification of nine potential SGD plumes. At the same locations, plumes were identified during the sonar surveys. In addition, fuzzy logic was used for the fusion of salinity, DO, and FDOM readings in order to enhance SGD detection capability of the designed multi-sensor system. The fuzzy logic approach identified 293 data points as potential within a SGD plume. Average minimum-distance between these points and the identified SGD plumes was 0.5 m and 0.42 m smaller than the minimum-distance average of the remaining data points of survey one and three respectively. It was shown that low-cost ROVs, equipped with environmental sensors, could be an important tool for the investigation of the spatio-temporal behaviour of SGD sites. This method allows continuous mapping of environmental parameters with a high spatial and temporal resolution. However, to obtain deeper insights into the influence of SGDs on the nearshore areas, this method should be combined with other well-established methods for SGD investigation, such as pore water sampling, remote sensing, or groundwater monitoring.
Low-Density Polyethylene Film Biodegradation Potential by Fungal Species from Thailand
Authors: Khruengsai Sarunpron , Sripahco Teerapong , Pripdeevech Patcharee
Published: July 2021

Accumulated plastic waste in the environment is a serious problem that poses an ecological threat. Plastic waste has been reduced by initiating and applying different alternative methods from several perspectives, including fungal treatment. Biodegradation of 30 fungi from Thailand were screened in mineral salt medium agar containing low-density polyethylene (LDPE) films. Diaporthe italiana, Thyrostroma jaczewskii, Collectotrichum fructicola, and Stagonosporopsis citrulli were found to grow significantly by culturing with LDPE film as the only sole carbon source compared to those obtained from Aspergillus niger. These fungi were further cultured in mineral salt medium broth containing LDPE film as the sole carbon source for 90 days. The biodegradation ability of these fungi was evaluated from the amount of CO2 and enzyme production. Different amounts of CO2 were released from D. italiana, T. jaczewskii, C. fructicola, S. citrulli, and A. niger culturing with LDPE film, ranging from 0.45 to 1.45, 0.36 to 1.22, 0.45 to 1.45, 0.33 to 1.26, and 0.37 to 1.27 g/L, respectively. These fungi were able to secrete a large amount of laccase enzyme compared to manganese peroxidase, and lignin peroxidase enzymes detected under the same conditions. The degradation of LDPE films by culturing with these fungi was further determined. LDPE films cultured with D. italiana, T. jaczewskii, C. fructicola, S. citrulli, and A. niger showed weight loss of 43.90%, 46.34%, 48.78%, 45.12%, and 28.78%, respectively. The tensile strength of LDPE films cultured with D. italiana, T. jaczewskii, C. fructicola, S. citrulli, and A. niger also reduced significantly by 1.56, 1.78, 0.43, 1.86, and 3.34 MPa, respectively. The results from Fourier transform infrared spectroscopy (FTIR) reveal an increasing carbonyl index in LDPE films culturing with these fungi, especially C. fructicola. Analysis of LDPE films using scanning electron microscopy (SEM) confirmed the biodegradation by the presence of morphological changes such as cracks, scions, and holes on the surface of the film. The volatile organic compounds (VOCs) emitted from LDPE films cultured with these fungi were analyzed by gas chromatography-mass spectrometry (GC-MS). VOCs such as 1,3-dimethoxy-benzene, 1,3-dimethoxy-5-(1-methylethyl)-benzene, and 1,1-dimethoxy-decane were detected among these fungi. Overall, these fungi have the ability to break down and consume the LDPE film. The fungus C. fructicola is a promising resource for the biodegradation of LDPE which may be further applied in plastic degradation systems based on fungi.
Azole-Resistance Development; How the Aspergillus fumigatus Lifecycle Defines the Potential for Adaptation
Authors: Zhang Jianhua , Debets Alfons J. M. , Verweij Paul E. , Snelders Eveline
Published: July 2021

In order to successfully infect or colonize human hosts or survive changing environments, Aspergillus fumigatus needs to adapt through genetic changes or phenotypic plasticity. The genomic changes are based on the capacity of the fungus to produce genetic variation, followed by selection of the genotypes that are most fit to the new environment. Much scientific work has focused on the metabolic plasticity, biofilm formation or the particular genetic changes themselves leading to adaptation, such as antifungal resistance in the host. Recent scientific work has shown advances made in understanding the natural relevance of parasex and how both the asexual and sexual reproduction can lead to tandem repeat elongation in the target gene of the azoles: the cyp51A gene. In this review, we will explain how the fungus can generate genetic variation that can lead to adaptation. We will discuss recent advances that have been made in the understanding of the lifecycle of A. fumigatus to explain the differences observed in speed and type of mutations that are generated under different environments and how this can facilitate adaptation, such as azole-resistance selection.
Production and Activity of Cristazarin in the Lichen-Forming Fungus Cladonia metacorallifera
Authors: Jeong Min-Hye , Park Chan-Ho , Kim Jung A , Choi Eu Ddeum , Kim Soonok , Hur Jae-Seoun , Park Sook-Young
Published: July 2021

Lichens are a natural source of bioactive compounds. Cladonia metacorallifera var. reagens KoLRI002260 is a rare lichen known to produce phenolic compounds, such as rhodocladonic, thamnolic, and didymic acids. However, these metabolites have not been detected in isolated mycobionts. We investigated the effects of six carbon sources on metabolite biosynthesis in the C. metacorallifera mycobiont. Red pigments appeared only in Lilly and Barnett’s media with fructose at 15 °C after 3 weeks of culture and decreased after 6 weeks. We purified these red pigments using preparative-scale high performance liquid chromatography and analyzed them via nuclear magnetic resonance. Results indicated that 1% fructose-induced cristazarin and 6-methylcristazarin production under light conditions. In total, 27 out of 30 putative polyketide synthase genes were differentially expressed after 3 weeks of culture, implying that these genes may be required for cristazarin production in C. metacorallifera. Moreover, the white collar genes Cmwc-1 and Cmwc-2 were highly upregulated at all times under light conditions, indicating a possible correlation between cristazarin production and gene expression. The cancer cell lines AGS, CT26, and B16F1 were sensitive to cristazarin, with IC50 values of 18.2, 26.1, and 30.9 μg/mL, respectively, which highlights the value of cristazarin. Overall, our results suggest that 1% fructose under light conditions is required for cristazarin production by C. metacorallifera mycobionts, and cristazarin could be a good bioactive compound.
Folate Receptor Alpha Autoantibodies in Autism Spectrum Disorders: Diagnosis, Treatment and Prevention
Authors: Bobrowski-Khoury Natasha , Ramaekers Vincent T. , Sequeira Jeffrey M. , Quadros Edward V.
Published: July 2021

Folate deficiency and folate receptor autoimmune disorder are major contributors to infertility, pregnancy related complications and abnormal fetal development including structural and functional abnormalities of the brain. Food fortification and prenatal folic acid supplementation has reduced the incidence of neural tube defect (NTD) pregnancies but is unlikely to prevent pregnancy-related complications in the presence of folate receptor autoantibodies (FRAb). In pregnancy, these autoantibodies can block folate transport to the fetus and in young children, folate transport to the brain. These antibodies are prevalent in neural tube defect pregnancies and in developmental disorders such as cerebral folate deficiency (CFD) syndrome and autism spectrum disorder (ASD). In the latter conditions, folinic acid treatment has shown clinical improvement in some of the core ASD deficits. Early testing for folate receptor autoantibodies and intervention is likely to result in a positive outcome. This review discusses the first identification of FRAb in women with a history of neural tube defect pregnancy and FRAb’s association with sub-fertility and preterm birth. Autoantibodies against folate receptor alpha (FRα) are present in about 70% of the children with a diagnosis of ASD, and a significant number of these children respond to oral folinic acid with overall improvements in speech, language and social interaction. The diagnosis of folate receptor autoimmune disorder by measuring autoantibodies against FRα in the serum provides a marker with the potential for treatment and perhaps preventing the pathologic consequences of folate receptor autoimmune disorder.
Forest Transition and Its Dynamics in Subtropical Chongqing, China since 1990s
Authors: Li Lingyue , Qi Zhixin , Zhong Teng
Published: July 2021

This research aims to advance our understanding towards forest transition, which is about the shift from net deforestation to net reforestation over a given area during certain period, through a case study of a western city in China from 1990 to 2015. Three main contributions are made to the theory and practice of forest land management. First, this research enriches forest transition theory with a meso-level exploration on forest land change in subtropic Chongqing, which echoes the “U” shape transition rules widely observed in the Euro-American context but was found a time lag of the turning point until 2000s. Second, it intentionally identifies the subtype of forests—the artificial plantation, which is considered influential on performance of forest’s carbon sequestration but not paid sufficient attention to. Third, it adds knowledge on forest transition pathway at an intra-urban scale through the identified significance degree of forest transition dynamics, which implies that economic development matters but is less important than topography in a mountainous city like Chongqing, and different dimensions of economy impact differently on forest transition.
Analysis and Evaluation of the Spatial Structure of Cittaslow Towns on the Example of Selected Regions in Central Italy and North-Eastern Poland
Authors: Zagroba Marek , Pawlewicz Katarzyna , Senetra Adam
Published: July 2021

Cittaslow International promotes harmonious development of small towns based on sustainable relationships between economic growth, protection of local traditions, cultural heritage and the environment, and an improvement in the quality of local life. The aim of this study was to analyze and evaluate the differences and similarities in the spatial structure of Cittaslow towns in the Italian regions of Tuscany and Umbria and the Polish region of Warmia and Mazury. The study examined historical towns which are situated in different parts of Europe and have evolved in different cultural and natural environments. The presented research attempts to determine whether the spatial structure of historical towns established in different European regions promotes the dissemination of the Cittaslow philosophy and the adoption of sustainable development principles. The urban design, architectural features and the composition of urban and architectural factors which are largely responsible for perceptions of multi-dimensional space were evaluated. These goals were achieved with the use of a self-designed research method which supported a subjective evaluation of spatial structure defined by historical urban planning and architectural solutions. The study demonstrated that Medieval urban layouts can be successfully incorporated into the modern urban fabric to promote sustainable development and slow living.
Research on the Impact of Land Circulation on the Income Gap of Rural Households: Evidence from CHIP
Authors: Huo Congjia , Chen Lingming
Published: July 2021

With the continued development of the economy, the income gap among Chinese rural households continues to widen. The land system plays a decisive role in developing “agriculture, rural areas and farmers” and land circulation is a factor in the increase in income inequality among farm households. Based on the 2013 China Household Income Project (CHIP), this article used the re-centered influence function (RIF) regression method to empirically test the impact of rural land circulation on the income gap of rural households in China in three regions: the central, eastern and western regions. The quantile regression tested the impact mechanism of income inequality of rural households from the perspective of labor mobility and land circulation. The empirical results showed that land circulation increases the income inequality of rural households. The theoretical mechanism test proved that the dynamic relationship between land circulation and labor mobility increases rural household income. However, this increase has a greater effect on rural households with a high income and a small effect on rural households with a low income, resulting in a further widening of the income gap. Therefore, while increasing the income of rural households through land circulation, the government should also consider income equity. Finally, this article puts forward the policies and opinions on land reform and provides a brief discussion on the future direction of development.
Restorative Practices in Institutional Settings: The Challenges of Contractualised Support within the Managed Community of Supported Housing
Authors: Hobson Jonathan , Payne Brian , Lynch Kenneth , Hyde Darren
Published: July 2021

Supported housing services provide help to some of the most vulnerable in society, yet across the world face increasing pressures from residualisation in the form of service reduction and stretched budgets. In response to these challenges, providers of supported housing and other similar community-focused services have sought alternative and innovative methods of engagement. This paper reports on one such example, the Restorative Communities Programme, which took place in 2018 in a residential supported housing setting for males aged 16–25. Designed as a five-week programme, it aimed to offer a proactive intervention promoting the benefits of restorative thinking. The research team observed the sessions and conducted follow-up qualitative interviews after two weeks and one year. This paper considers the challenges and success of the programme, reflecting particularly on issues of contractualised support and its impact on participation, and the dynamics of running such programs within the ‘managed community’ of a supported housing project. As such, the paper provides a useful analysis for others exploring the development and use of restorative projects in institutional settings, such as prisons, probation, and schools, and particularly those services struggling with the pressures of residualisation.