bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical and Molecular Features of Skin Malignancies in Muir-Torre Syndrome
Authors: Simic Dario , Dummer Reinhard , Freiberger Sandra N. , Ramelyte Egle , Barysch Marjam-Jeanette
Published: May 2021

Background: We investigated the mutational landscape of skin tumors in patients with Muir-Torre Syndrome (MTS) a hereditary autosomal dominant mismatch repair disorder of increased cancer susceptibility, and examined mutations other than in the DNA mismatch repair (MMR) genes. Methods: This retrospective single-center case series included seven patients with the diagnosis of Muir-Torre Syndrome with precise medical history and family history. Mutational analysis of tumor samples Formalin-fixed paraffin-embedded tissue blocks of skin lesions associated with Muir-Torre Syndrome were used for further analysis. All skin tumors were analyzed with the Oncomine Comprehensive Assay v3 (Life Technologies), which includes 161 of the most relevant cancer driver genes. Results: Eleven skin neoplasms (nine sebaceous tumors, one melanoma, one cutaneous squamous cell carcinoma) were diagnosed in seven patients. In two patients, visceral malignancies preceded the diagnosis of the skin tumors and one patient was diagnosed with a visceral malignancy after a sebaceous tumor. History of familial cancer of Lynch Syndrome (LS) was reported in three patients. The most frequently detected mutation was in the MSH2 gene, followed by mutations in the NOTCH1/2 and TP53 gene. Conclusion, this study provides a molecular analysis of Muir-Torre Syndrome associated and non-associated skin tumors in patients with Muir-Torre Syndrome. Patients with sebaceous lesions should undergo microsatellite instability analysis and accurate evaluation of personal and family history to detect a possible Muir-Torre syndrome. As secondary malignancies may appear years after the first occurrence of sebaceous tumors, lifelong screening is mandatory.
Harnessing Crop Wild Diversity for Climate Change Adaptation
Authors: Cortés Andrés J. , López-Hernández Felipe
Published: May 2021

Warming and drought are reducing global crop production with a potential to substantially worsen global malnutrition. As with the green revolution in the last century, plant genetics may offer concrete opportunities to increase yield and crop adaptability. However, the rate at which the threat is happening requires powering new strategies in order to meet the global food demand. In this review, we highlight major recent ‘big data’ developments from both empirical and theoretical genomics that may speed up the identification, conservation, and breeding of exotic and elite crop varieties with the potential to feed humans. We first emphasize the major bottlenecks to capture and utilize novel sources of variation in abiotic stress (i.e., heat and drought) tolerance. We argue that adaptation of crop wild relatives to dry environments could be informative on how plant phenotypes may react to a drier climate because natural selection has already tested more options than humans ever will. Because isolated pockets of cryptic diversity may still persist in remote semi-arid regions, we encourage new habitat-based population-guided collections for genebanks. We continue discussing how to systematically study abiotic stress tolerance in these crop collections of wild and landraces using geo-referencing and extensive environmental data. By uncovering the genes that underlie the tolerance adaptive trait, natural variation has the potential to be introgressed into elite cultivars. However, unlocking adaptive genetic variation hidden in related wild species and early landraces remains a major challenge for complex traits that, as abiotic stress tolerance, are polygenic (i.e., regulated by many low-effect genes). Therefore, we finish prospecting modern analytical approaches that will serve to overcome this issue. Concretely, genomic prediction, machine learning, and multi-trait gene editing, all offer innovative alternatives to speed up more accurate pre- and breeding efforts toward the increase in crop adaptability and yield, while matching future global food demands in the face of increased heat and drought. In order for these ‘big data’ approaches to succeed, we advocate for a trans-disciplinary approach with open-source data and long-term funding. The recent developments and perspectives discussed throughout this review ultimately aim to contribute to increased crop adaptability and yield in the face of heat waves and drought events.
Genetic Risk Factors in Early-Onset Nonalcoholic Chronic Pancreatitis: An Update
Authors: Wertheim-Tysarowska Katarzyna , Oracz Grzegorz , Rygiel Agnieszka Magdalena
Published: May 2021

Chronic pancreatitis (CP) is a progressive, irreversible inflammatory disorder of the pancreas, which results from interrelations between different genetic and environmental factors. Genetic variants are the primary cause of the disease in early-onset nonalcoholic CP patients. Novel CP-associated genes are continuously emerging from genetic studies on CP cohorts, providing important clues for distinct mechanisms involved in CP development. On the basis of functional studies, the genetic alterations have been sub-grouped into CP-driving pathological pathways. This review focuses on the concept of CP as a complex disease driven by multiple genetic factors. We will discuss only well-defined genetic risk factors and distinct functional pathways involved in CP development, especially in the context of the early-onset nonalcoholic CP group. The diagnostic implications of the genetic testing will be addressed as well.
Characterization of Adobe Blocks: Point-Load Assessment as a Complementary Study of Damaged Buildings and Samples
Authors: Sanchez-Calvillo Adria , Alonso-Guzman Elia M. , Martinez-Molina Wilfrido , Navarrete-Seras Marco A. , Ruvalcaba-Sil Jose L. , Navarro-Ezquerra Antonia , Mitrani Alejandro
Published: May 2021

Adobe masonry is one of the oldest construction systems still in use today, Mexico has an enormous cultural heritage with traditional adobe houses being very representative of the rural communities and their culture. The 2017 Puebla Earthquake on September 19th struck the country causing the loss, destruction, and damage of historic buildings in several Mexican states, with the traditional earthen dwellings being the most vulnerable structures to these events. The fast abandonment of the local materials and techniques entails further research regarding the characterization of these construction systems, therefore, reconstruction efforts first require the recovery of the construction technique. After the seismic events, adobe samples of the remaining adobe structures of Jojutla de Juarez were collected. This population was one of the most affected in all the country, and, because of the major losses suffered, the study was conducted to determine the material properties of the dwellings’ adobe shards and natural quarry clays of the region. The characterization included destructive and non-destructive tests, mineralogical and granulometry analyses, and composition of the adobe samples of the buildings, as well as the aggregates. As a novelty, the compressive strength of the pieces was tested by two methods: the traditional compression strength test and the point-load test, in order to obtain the indicative values and the correlation equations between both tests. From the formal analysis and the laboratory, it was observed that the adobes from Jojutla presented different compositions which combined with the building malpractices and alterations to the traditional systems caused unpredictable behavior during the earthquake. The conduction of point-load tests in situ, as a part of a complete characterization methodology, could be an alternative to study the mechanical properties of patrimonial or damaged building samples before its disappearance.
Heat Treatment in Two Tomato Cultivars: A Study of the Effect on Physiological and Growth Recovery
Authors: Rajametov Sherzod Nigmatullaevich , Yang Eun Young , Jeong Hyo Bong , Cho Myeong Cheoul , Chae Soo Young , Paudel Niroj
Published: May 2021

High temperature (HT) significantly affects crop physiological traits and reduces productivity in plants. To increase yields as well as survival of crops under HT, developing heat-tolerant plants is one of the main targets in crop breeding programs. The present study attempted to investigate the linkage of the heat tolerance between the seedling and reproductive growth stages of tomato cultivars ’Dafnis‘ and ’Minichal.’ This research was undertaken to evaluate heat tolerance under two experimental designs such as screening at seedling stage and screening from reproductive traits in greenhouses. Survival rate and physiological responses in seedlings of tomatoes with 4-5 true leaves were estimated under HT (40 °C, RH 70%, day/night, respectively) and under two control and HT greenhouse conditions (day time 28 °C and 40 °C, respectively). Heat stress significantly affected physiological–chemical (photosynthesis, electrolyte conductivity, proline) and vegetative parameters (plant height, shoot fresh weight, root fresh weight) in all tomato seedlings. The findings revealed that regardless of tomato cultivars the photosynthesis, chlorophyll, total proline and electrical conductivity parameters were varied in seedlings during the heat stress period. The heat tolerance rate of tomatoes in the seedling stage might not always be associated with reproductive parameters. HT reduced fruit parameters such as fruit weight (31.9%), fruit length (14.1%), fruit diameter (19.1%), and fruit hardness (9.1%) compared to NT under HT in heat-susceptible tomato cultivar ‘Dafnis’, while in heat-tolerant cultivar ‘Minichal’ fruit length (7.1%) and fruit diameter (12.1%) was decreased by the effects of HT, but on the contrary fruit weight (3.6%) and fruit hardness (8.3%) were increased. In conclusion, screening and selection for tomatoes should be evaluated at the vegetative and reproductive stages with consideration of reproductive parameters.
Effects of a Tai Chi-Based Stroke Rehabilitation Program on Symptom Clusters, Physical and Cognitive Functions, and Quality of Life: A Randomized Feasibility Study
Authors: Song Rhayun , Park Moonkyoung , Jang Taejeong , Oh Jiwon , Sohn Min Kyun
Published: May 2021

Stroke survivors suffer from disease-associated symptoms. Tai Chi can be a beneficial approach to provide an adapted form of intervention to manage their symptoms. The study aimed to determine the effects of a Tai Chi-based stroke rehabilitation program on symptom clusters, physical and cognitive functions, and stroke-specific quality of life among stroke survivors in Korea. Thirty-four stroke survivors were randomly assigned to receive either the Tai Chi-based program or the stroke-symptom management program. The feasibility of the program and its effects on the outcomes were assessed at baseline, 3 months, and 6 months. Repeated measures ANOVA showed that most symptoms improved in both groups during the 6-month period, but swallowing-related symptoms improved significantly in the Tai Chi group. Based on the interaction effect, Tai Chi was more effective on flexor muscle strength, ambulation, and activities of daily living and cognitive function over 6 months than their counterparts. Among SS-QOL dimensions, the Tai Chi group showed significant improvements in the thinking and self-care dimensions. The Tai Chi-based stroke rehabilitation program was feasible and safely applicable to stroke survivors in the community settings. This program could improve symptoms, physical and cognitive function, leading to improvements in the self-care dimension of the SS-QOL among stroke survivors.
Bioelectrical Impedance Vector Analysis: A Valuable Tool to Monitor Daily Body Hydration Dynamics at Altitude
Authors: Regli Ivo B. , Turner Rachel , Woyke Simon , Rauch Simon , Brugger Hermann , Gatterer Hannes
Published: May 2021

Bioelectrical impedance vector analysis (BIVA) is a method used to estimate variation in body hydration. We assessed the potential of BIVA for monitoring daily body hydration fluctuations in nine healthy, normally active males under matching normoxic (NX) and hypobaric hypoxic (HH) experimental conditions. Furthermore, we aimed to investigate whether changes in BIVA may correspond with the development of acute mountain sickness (AMS). Subjects were exposed in a hypobaric chamber to both NX (corresponding to an altitude of 262 m) and HH conditions corresponding to an altitude of 3500 m during two four-day sojourns within which food, water intake and physical activity were controlled. Bioimpedance and body weight measurements were performed three times a day and medical symptoms were assessed every morning using the Lake Louise score (LLS). Total body water (TBW) was also assessed on the last day of both sojourns using the deuterium dilution technique. We detected circadian changes in vector length, indicating circadian body water variations that did not differ between NX and HH conditions (ANOVA effects: time: p = 0.018, eta2 = 0.149; interaction: p = 0.214, eta2 = 0.083; condition: p = 0.920, eta2 = 0.001). Even though none of the subjects developed AMS, four subjects showed clinical symptoms according to the LLS during the first 24 hours of HH conditions. These subjects showed a pronounced (Cohen’s d: 1.09), yet not statistically significant (p = 0.206) decrease in phase angle 6 hours after exposure, which may indicate fluid shift from the intracellular to the extracellular compartment. At the end of each sojourn, vector length correlated with deuterium dilution TBW “gold standard” measurements (linear regression: NX: p = 0.002 and r2 = 0.756, HH: p < 0.001 and r2 = 0.84). BIVA can be considered a valuable method for monitoring body hydration changes at altitude. Whether such changes are related to the development of clinical symptoms associated with AMS, as indicated in the present investigation, must be confirmed in future studies.
Technostress of Chilean Teachers in the Context of the COVID-19 Pandemic and Teleworking
Authors: Estrada-Muñoz Carla , Vega-Muñoz Alejandro , Castillo Dante , Müller-Pérez Sheyla , Boada-Grau Joan
Published: May 2021

This article shows the levels of technostress in primary and secondary education teachers in Chile, in the context of educational telework that Chile has adopted in connection with the health crisis by COVID-19. The information has been collected with the use of the RED-TIC scale, previously used in this country, whose validity and reliability of the instrument has been treated, for this case, with confirmatory factorial analysis (CFA) with a national coverage sample of 3006 teachers. The results show that 11% of teachers reveal techno anxiety and 7.2%, techno fatigue. Combining both manifestations, we find that 6.8% of teachers are techno stressed. Finally, fatigue and anxiety factors are higher for female teachers.
Comment on Choi et al. Cellular Phone Use and Risk of Tumors: Systematic Review and Meta-Analysis. Int. J. Environ. Res. Public Health 2020, 17, 8079
Authors: Brzozek Christopher , Abramson Michael J. , Benke Geza , Karipidis Ken
Published: May 2021

In September 2020, Choi et al. published their paper “Cellular Phone Use and Risk of Tumors: Systematic Review and Meta-Analysis” in the International Journal of Environmental Research and Public Health [...]
Use of Coronary Computed Tomography Angiography to Screen Hospital Employees with Cardiovascular Risk Factors
Authors: Li Po-Yi , Chen Ru-Yih , Wu Fu-Zong , Mar Guang-Yuan , Wu Ming-Ting , Wang Fu-Wei
Published: May 2021

The objective of this study was to determine how coronary computed tomography angiography (CCTA) can be employed to detect coronary artery disease in hospital employees, enabling early treatment and minimizing damage. All employees of our hospital were assessed using the Framingham Risk Score. Those with a 10-year risk of myocardial infarction or death of >10% were offered CCTA; the Coronary Artery Disease Reporting and Data System (CAD-RADS) score was the outcome. A total of 3923 hospital employees were included, and the number who had received CCTA was 309. Among these 309, 31 (10.0%) had a CAD-RADS score of 3–5, with 10 of the 31 (32.3%) requiring further cardiac catheterization; 161 (52.1%) had a score of 1–2; and 117 (37.9%) had a score of 0. In the multivariate logistic regression, only age of ≥ 55 years (p < 0.05), hypertension (p < 0.05), and hyperlipidemia (p < 0.05) were discovered to be significant risk factors for a CAD-RADS score of 3–5. Thus, regular and adequate control of chronic diseases is critical for patients, and more studies are required to be confirmed if there are more significant risk factors.
Association between Tobacco Consumption and Problematic Internet Use and the Practice of Physical Activity in Spanish Adolescents
Authors: Villanueva-Blasco Víctor José , García-Soidán José Luis , Isorna Folgar Manuel , Arufe Giráldez Víctor
Published: May 2021

The practice of physical activity (PA) is a healthy habit that offers health benefits. In contrast, the lack thereof may be associated with an increase in diseases, even at an early age. The objective of this study was to analyze the association between unhealthy behaviors, such as tobacco consumption and problematic internet use, and the practice of PA in adolescents. Protective factors (physical activity and sport) and risk factors (leading a sedentary life, tobacco use, and problematic internet use) were evaluated. Other variables such as sex, the intensity of physical activity, and being a member of a sports federation were also evaluated. The sample consisted of a total of 1222 Spanish adolescents. Univariate descriptive analysis and multiple linear regression were used, and confirmatory factor analyses and structural models were also estimated. The results confirm a significant positive association between physical activity, intensity, and being a member of a sports federation, as well as between cigarette consumption and internet use. It is advisable to implement public policies that promote the practice of sports as a direct investment in health, preventing the consumption of tobacco and other habits that are harmful to the health of adolescents.
How Does Metro Maintenance Staff’s Risk Perception Influence Safety Citizenship Behavior—The Mediating Role of Safety Attitude
Authors: Zhai Huaiyuan , Li Mengjie , Hao Shengyue , Chen Mingli , Kong Lingchen
Published: May 2021

The accident rate is high in subway maintenance work, and most of the accidents are caused by human factors, especially the lack of sensitivity to risk perception, the lack of rigorous attitude towards safety and the lack of safe citizenship behavior (SCB). Therefore, it is very important to study the risk perception (RP), safety attitude (SA) and SCB of metro maintenance staff in order to reduce the accident rate. In order to reduce human errors and accidents, this study analyzed the influence of metro maintenance staff’s RP on their SCB and the mediating role of SA. Based on previous studies, this paper uses the risk perception scale, safety attitude scale and safety citizenship behavior scale as research tools. A survey was administered at the Subway Company, and altogether 268 valid questionnaires were used, and the data were analyzed by SPSS19.0 (IBM, Armonk, NY, USA) and AMOS 24.0 (IBM, Armonk, New York, NY, USA). The result reveals that SA plays a complete mediating role between metro maintenance staff’s RP and their SCB; and SA has a positive influence on SCB; RP has a positive influence on SA; and SA positively predicts SCB.
Employee Engagement and Wellbeing in Times of COVID-19: A Proposal of the 5Cs Model
Authors: De-la-Calle-Durán María-Carmen , Rodríguez-Sánchez José-Luis
Published: May 2021

The COVID-19 pandemic has had an unprecedented impact on the labor market. The psychological pressure and uncertainty caused by the current changing workplace environment have led to negative consequences for workers. Considering the predictive relationship between employee engagement and wellbeing and in light of this unprecedented situation that affects workers of all the industries worldwide, this study aims to identify the key main drivers of employee engagement that can lead to employee wellbeing in the current context. Through a literature review, a theoretical model to strengthen engagement in times of COVID-19 is proposed. The main factors are conciliation, cultivation, confidence, compensation, and communication. Whereas prior to the pandemic, firms had already understood the need to achieve this, it is now considered a vital tool for staff health and wellbeing. This article makes two main contributions. First, it provides a model for boosting employee engagement, and therefore, wellbeing. Second, managerial suggestions are made to apply the theoretical model.
The Prevalence of and Risk Factors Associated with Musculoskeletal Disorders in Thai Oil Palm Harvesting Workers: A Cross-Sectional Study
Authors: Bhuanantanondh Petcharatana , Buchholz Bryan , Arphorn Sara , Kongtip Pornpimol , Woskie Susan
Published: May 2021

Musculoskeletal disorders (MSDs) are common in various occupations. However, there is still limited research about the prevalence of, and risk factors associated with, MSDs among oil palm harvesting workers in Thailand. To investigate the prevalence of MSDs and risk factors associated with MSDs in Thai oil palm harvesting workers, face-to-face interviews were conducted with Thai oil palm harvesting workers in Krabi Province, Thailand, using a questionnaire. The questionnaire consisted of four sections which included information on demographic characteristics, work-related characteristics, job stress, and MSDs. A total of 334 oil palm harvesting workers participated in the current study. The prevalence of MSDs during the past 12 months was 88.0%. Lower back MSDs had the highest (59.0%) 12-month prevalence among oil palm harvesting workers, followed by shoulder (37.1%) and neck (27.2%). Factors associated with lower back MSDs included type of task, heavy lifting, and job stress. Moreover, type of task, repetitive movement, and job stress were associated with shoulder and neck MSDs. The cutters had a higher risk of having shoulder and neck MSDs, primarily due to the fact that their work involved cutting the fresh fruit bunches from high up in the trees. The collectors had more back issues due to the heavy lifting. These findings showed the need to raise awareness, and to design guidelines and interventions to prevent MSDs in oil palm harvesting workers.
Skin Cancer Detection: A Review Using Deep Learning Techniques
Authors: Dildar Mehwish , Akram Shumaila , Irfan Muhammad , Khan Hikmat Ullah , Ramzan Muhammad , Mahmood Abdur Rehman , Alsaiari Soliman Ayed , Saeed Abdul Hakeem M , Alraddadi Mohammed Olaythah , Mahnashi Mater Hussen
Published: May 2021

Skin cancer is one of the most dangerous forms of cancer. Skin cancer is caused by un-repaired deoxyribonucleic acid (DNA) in skin cells, which generate genetic defects or mutations on the skin. Skin cancer tends to gradually spread over other body parts, so it is more curable in initial stages, which is why it is best detected at early stages. The increasing rate of skin cancer cases, high mortality rate, and expensive medical treatment require that its symptoms be diagnosed early. Considering the seriousness of these issues, researchers have developed various early detection techniques for skin cancer. Lesion parameters such as symmetry, color, size, shape, etc. are used to detect skin cancer and to distinguish benign skin cancer from melanoma. This paper presents a detailed systematic review of deep learning techniques for the early detection of skin cancer. Research papers published in well-reputed journals, relevant to the topic of skin cancer diagnosis, were analyzed. Research findings are presented in tools, graphs, tables, techniques, and frameworks for better understanding.
Development of a Dust Respirator by Improving the Half Mask Frame Design
Authors: Bazaluk Oleg , Cheberiachko Serhii , Cheberiachko Yurii , Deryugin Oleh , Lozynskyi Vasyl , Knysh Ivan , Saik Pavlo , Naumov Mykola
Published: May 2021

Protective efficiency of filtering dust respirators depends on the properties of filter materials from which filters are made and the structure of a half mask frame, which influences how tightly the respirator fits the face. The conducted studies on the “Lepestok 40” dust respirator revealed a large air leakage through the gaps occurring along the obturation strip. Therefore, the purpose of the study is to develop a dust respirator to provide high level of protection and usability by improving the half mask frame design. A scheme for designing a dust respirator: analysis of operating conditions for the dust respirator; facial anthropometric measurements of potential users; designing a 3D model of half mask frame; laboratory testing of the protective properties of the product. A distinctive feature of this approach is considering the facial anthropometric dimensions of employees of a particular enterprise, standard sizes formation of 3D facial models, which is the basis for designing a half mask frame for dust respirator. A new half mask frame design for dust respirator with a variable geometry of fitting to the face surface has been developed, due to special attachment points that allow changing its size according to the anthropometric dimensions of user’s face.
The Impact of Pandemic Management on the Quality of Life of Slovak Dentists
Authors: Pacutova Veronika , Madarasova Geckova Andrea , Kizek Peter , F. de Winter Andrea , A. Reijneveld Sijmen
Published: May 2021

Pandemic management increases the burden on healthcare workers to provide care and also affects their personal lives, with dentists being at particular risk. Therefore, we aim to describe the quality of life (QoL) and limitations experienced due to pandemic management-related measures (PanMan), as well as to assess the association of PanMan with QoL during the first lockdown after the coronavirus outbreak. We obtained data from 500 dentists (33.2% males, M/SD = 43.8) registered with the Slovak Chamber of Dentists using an online questionnaire. We categorized PanMan as the availability of personal protective equipment (PPE) and the ability to implement anti-pandemic measures, information overload, pandemic-related limitations and QoL in terms of their impact on family life and activities, housekeeping, relationships with relatives, financial situation and mental well-being. PanMan mainly affected financial situation, mental well-being and housekeeping. Factors contributing most towards the worsening of QoL were information overload (odds ratio/95% confidence interval, OR/CI: 5.79/2.64–12.71) and several pandemic-related limitations. These consisted of (OR/CI): a lack of PPE (5.17/2.48–10.77), infection risks in the work environment (3.06/1.57–5.95), obligatory safety measures (3.02/1.47–6.21), lack of staff (2.85/1.30–6.25) and client concerns (3.56/1.70–7.49). Pandemic management has led to a considerable worsening of dentists’ QoL.
4BR: Educational Training Programme for the Prevention of Sports Injuries in Young Athletes
Authors: Palmi Joan , Alcubierre Nuria , Gil Moreno de Mora Gonzalo , Reig Francesc , Planas-Anzano Antoni
Published: May 2021

This article provides a vision of the importance of the recovery process for the prevention of injuries in young athletes. From a sports psychology perspective, it presents a proposal for an optimisation programme to reduce the negative impact of exertion and subsequent risk of injury. The 4BR programme consists of three sub-programmes (technician advice, vulnerability detection, and the implementation of four recovery habit blocks). An interdisciplinary approach is taken to configure the four healthy blocks: nutrition–hydration, relaxation–rest, social life and personal moments. It demonstrates the importance of personalised adaptation to the sports context and moment in order to achieve maximum effectiveness of the proposed 4BR programme, which comprises workshops, exercises, evaluation systems and information feedback. The presented programme facilitates recovery, optimizes the return to training and reduces the risk of intrinsic injuries in young people. The conclusion drawn from the study is that there is a need to conduct further research to find empirical evidence of the positive effects of applying the 4BR programme to different sports.
Prevalence of Orthorexia Nervosa and Its Diagnostic Tools—A Literature Review
Authors: Niedzielski Antoni , Kaźmierczak-Wojtaś Natalia
Published: May 2021

The aim of this article is to present the up-to-date diagnostic tools of orthorexia and markers of its prevalence on the basis of the available literature. The authors searched PubMedCentral (PMC) and Google Scholar with the search entry of “orthorexia”, “orthorexia nervosa”, and “orthorexicbehaviours”. We describe the tools of evaluation of orthorexicbehaviour (i.e., orthorexia self-test—BOT, the ORTO-15 questionnaire, Eating Habits Questionnaire—EHQ, Düsseldorf Orthorexia Scale—DOS, Teruel Orthorexia Scale—TOS, Barcelona Orthorexia Scale—BOS, and Orthorexia Nervosa Inventory—ONI), and offer a review of the studies on orthorexia nervosa. We conclude that there are no reliable data regarding the prevalence of orthorexia nervosa. The available studies point to significant differences in the prevalence depending on the value of cut-off points and tools used. The prevalence varies across countries and across populations, ranging from 6.9% in the Italian population to 88.7% in the group of Brazilian students of dieting. Thus, it indicates that some groups seem to be susceptible to the risk of ON more than others. It is a challenge to determine the prevalence of orthorexia, and any obtained results should be treated with caution. Consequently, we claim that the use of the ORTO-15 questionnaire to diagnose orthorexia is questionable due to a high percentage of falsely positive results.
What Do Childcare Providers Know about Environmental Influences on Children’s Health? Implications for Environmental Health Literacy Efforts
Authors: Koester Brenda D. , Sloane Stephanie , Fujimoto Elinor M. , Fiese Barbara H. , Su Leona Yi-Fan
Published: May 2021

Children are uniquely vulnerable to toxicant exposures in their environment, which can have long-lasting impacts on their health. Childcare providers are an important population to target for environmental health literacy, as most children in the United States under five years of age spend a significant number of waking hours in non-parental care. There is an increasing body of evidence that children are exposed to toxicants in the childcare environment, and yet little is known about what childcare providers know about environmental influences on the health of children in their care. We conducted semi-structured interviews with 36 home- and center-based Illinois childcare providers to better understand their knowledge, attitudes, and behaviors as they relate to environmental influences on children’s health. We found that the majority of providers had a low level of understanding of potential sources of exposure in the childcare environment, and they did not feel that environmental exposures posed a significant risk to children. Future efforts to increase environmental health literacy should focus on raising awareness and knowledge of environmental health issues for childcare providers before addressing ways that providers can reduce or prevent toxicant exposures to children in their care.
Trajectories of Body Height, Body Weight, BMI, and Nutrition Status from 1979 to 1987: A Measurement-Based Analysis of 8740 Montenegrin Male Adolescents from the Municipality of Berane
Authors: Bjelica Dusko , Gardasevic Jovan , Milosevic Zoran , Bozic Predrag R. , Masanovic Bojan
Published: May 2021

This study aimed to consolidate the body height, body weight, BMI, and nutrition status data of the overall young male population from the Municipality of Berane in order to assess the trajectories of those variables from 1979 to 1987. The sample of respondents included 8740 adolescents who were divided into nine groups according to their age. The sample of variables included body height, body weight, body mass index, and nutrition status, which were presented based on a long-established BMI categorization (underweight, normal weight, pre-obese, and obese). The descriptive statistics are expressed as the mean and standard deviation for each variable; the analysis of nutrition status was calculated based on BMI, while LSD post hoc testing with ANOVA was employed to investigate differences between the means. The results indicate that a secular trend is visible regarding body height and body weight, while no trend is visible for the BMI and nutrition status. This study’s contribution is that it provides insight into more recently published data for the studied period and in this municipality, which can significantly aid in following the secular trend throughout Montenegro.
Shift Work and Dry Eye Disease in the Korean Working Population: A Population-Based Cross-Sectional Study
Authors: Ahn Joonho , Ryu So-Jung , Song Jihun , Kim Hyoung-Ryoul
Published: May 2021

This study aimed to evaluate the association between shift work and dry eye disease (DED) in the general population. The 2011 Korea Health Panel (KHP) was used. Chi-square test and multivariate logistic regression were used to assess the relationship between shift work and DED. Stratification analysis was conducted by sex and age. Overall, the odds ratio (OR) of DED according to shift work did not showed significant results (adjusted OR = 1.230, 95% CI 0.758–1.901). When findings were stratified based on age older or younger than 40 years, the OR of DED increased to 2.85 (95% CI: 1.25–5.90) in shift workers under 40 years of age. Our results show an association between shift work and DED in a group of younger subjects.
Effects of a Short Emotional Management Program on Inpatients with Schizophrenia: A Pilot Study
Authors: Park Kyung-Hwan , Park Eun-Sook , Jo Sung-Mi , Seo Mi-Hui , Song Young-Ok , Jang Sun-Joo
Published: May 2021

The prevalence of schizophrenia is gradually increasing worldwide. Many patients with schizophrenia have a diminished ability to empathize and to detect their own emotions or those of others, deteriorating their social functioning and their quality of life. Nonetheless, emotional management training may improve patients’ emotion recognition, emotional expression, and negative symptoms. Developing and applying a short but effective program that reflects the current medical environment, in which hospital stays are ever-diminishing, is warranted. This one-group, pretest–posttest, quasi-experimental pilot study aimed to examine the effects of a short emotional management program (EMP-S) on 17 patients with chronic schizophrenia. Participants were patients hospitalized in the National Center for Mental Health in Korea. After the completion of a twice-a-week, eight-session, four-week long EMP-S, participants showed improvements in emotion recognition, emotional expression, and negative symptoms. Our results suggest the applicability and potential effectiveness of the EMP-S, which takes the length of psychiatric hospital stay and the inpatient environment into consideration. To minimize any barriers to social functioning in the post-discharge lives of inpatients with chronic schizophrenia and enhance their social cognition—by improving their emotion recognition, emotional expression, and negative symptoms—we suggest the periodical administration of this EMP-S to these inpatients.
Public Bike Trip Purpose Inference Using Point-of-Interest Data
Authors: Lee Jiwon , Yu Kiyun , Kim Jiyoung
Published: May 2021

Public bike-sharing is eco-friendly, connects excellently with other transportation modes, and provides a means of mobility that is highly suitable in the current era of climate change. This study proposes a methodology for inferring the bike trip purpose based on bike-share and point-of-interest (POI) data. Because the purpose of a trip involves decision-making, its inference necessitates an understanding of the spatiotemporal complexity of human activities. Thus, the spatiotemporal features affecting bike trips were selected from the bike-share data, and the land uses at the origin and destination of the trips were extracted from the POI data. During POI type embedding, the data were augmented considering the geographical distance between the POIs and the number of bike rentals at each bike station. We further developed a ground truth data construction method that uses temporal mobile and POI data. The inference model was built using machine learning and applied to experiments involving bike stations in Seocho-gu, Seoul, Korea. The experimental results revealed that optimal performance was achieved with the use of decision tree algorithms, as demonstrated by a 78.95% overall accuracy and 66.43% F1-score. The proposed method contributes to a better understanding of the causes of movement within cities.
Silencing of the Slt2-Type MAP Kinase Bmp3 in Botrytis cinerea by Application of Exogenous dsRNA Affects Fungal Growth and Virulence on Lactuca sativa
Authors: Spada Maria , Pugliesi Claudio , Fambrini Marco , Pecchia Susanna
Published: May 2021

Botrytis cinerea can attack over 500 genera of vascular plants and is considered the second phytopathogen in the ‘top ten’ for its economic importance. Traditional fungicides can be ineffective and with increasing fungicide resistance, new sustainable technologies are required. Lately, RNA interference-based fungicides are emerging for their potential uses in crop protection. Therefore, we assessed the potential of this innovative approach targeting the MAP kinase Bmp3 in B. cinerea, a gene involved in saprophytic growth, response to low osmolarity, conidiation, surface sensing, host penetration and lesion formation. After performing a prediction analysis of small interfering RNAs, a 427 nucleotides long dsRNA was selected as construct. We tested the effect of topical applications of dsRNA construct both in vitro by a fungal growth assay in microtiter plates and in vivo on detached lettuce leaves artificially inoculated. In both cases, topical applications of dsRNA led to gene knockdown with a delay in conidial germination, an evident growth retardation and a strong reduction of necrotic lesions on leaves. These results correlated with a strongly reduced expression of Bmp3 gene. In accordance to these findings, the Bmp3 gene could be a promising target for the development of an RNAi-based fungicide against B. cinerea.
Tau Exon 10 Inclusion by PrPC through Downregulating GSK3β Activity
Authors: Lidón Laia , Llaó-Hierro Laura , Nuvolone Mario , Aguzzi Adriano , Ávila Jesús , Ferrer Isidro , del Río José Antonio , Gavín Rosalina
Published: May 2021

Tau protein is largely responsible for tauopathies, including Alzheimer’s disease (AD), where it accumulates in the brain as insoluble aggregates. Tau mRNA is regulated by alternative splicing, and inclusion or exclusion of exon 10 gives rise to the 3R and 4R isoforms respectively, whose balance is physiologically regulated. In this sense, one of the several factors that regulate alternative splicing of tau is GSK3β, whose activity is inhibited by the cellular prion protein (PrPC), which has different physiological functions in neuroprotection and neuronal differentiation. Moreover, a relationship between PrPC and tau expression levels has been reported during AD evolution. For this reason, in this study we aimed to analyze the role of PrPC and the implication of GSK3β in the regulation of tau exon 10 alternative splicing. We used AD human samples and mouse models of PrPC ablation and tau overexpression. In addition, we used primary neuronal cultures to develop functional studies. Our results revealed a paralleled association between PrPC expression and tau 4R isoforms in all models analyzed. In this sense, reduction or ablation of PrPC levels induces an increase in tau 3R/4R balance. More relevantly, our data points to GSK3β activity downstream from PrPC in this phenomenon. Our results indicate that PrPC plays a role in tau exon 10 inclusion through the inhibitory capacity of GSK3β.
Rapamycin Ameliorates Defects in Mitochondrial Fission and Mitophagy in Glioblastoma Cells
Authors: Lenzi Paola , Ferese Rosangela , Biagioni Francesca , Fulceri Federica , Busceti Carla L. , Falleni Alessandra , Gambardella Stefano , Frati Alessandro , Fornai Francesco
Published: May 2021

Glioblastoma (GBM) cells feature mitochondrial alterations, which are documented and quantified in the present study, by using ultrastructural morphometry. Mitochondrial impairment, which roughly occurs in half of the organelles, is shown to be related to mTOR overexpression and autophagy suppression. The novelty of the present study consists of detailing an mTOR-dependent mitophagy occlusion, along with suppression of mitochondrial fission. These phenomena contribute to explain the increase in altered mitochondria reported here. Administration of the mTOR inhibitor rapamycin rescues mitochondrial alterations. In detail, rapamycin induces the expression of genes promoting mitophagy (PINK1, PARKIN, ULK1, AMBRA1) and mitochondrial fission (FIS1, DRP1). This occurs along with over-expression of VPS34, an early gene placed upstream in the autophagy pathway. The topographic stoichiometry of proteins coded by these genes within mitochondria indicates that, a remarkable polarization of proteins involved in fission and mitophagy within mitochondria including LC3 takes place. Co-localization of these proteins within mitochondria, persists for weeks following rapamycin, which produces long-lasting mitochondrial plasticity. Thus, rapamycin restores mitochondrial status in GBM cells. These findings add novel evidence about mitochondria and GBM, while fostering a novel therapeutic approach to restore healthy mitochondria through mTOR inhibition.
Hydrogen Bonds: Raman Spectroscopic Study
Authors: Kolesov Boris
Published: May 2021

The work outlines general ideas on how the frequency and the intensity of proton vibrations of X–H···Y hydrogen bonding are formed as the bond evolves from weak to maximally strong bonding. For this purpose, the Raman spectra of different chemical compounds with moderate, strong, and extremely strong hydrogen bonds were obtained in the temperature region of 5 K–300 K. The dependence of the proton vibrational frequency is schematically presented as a function of the rigidity of O-H···O bonding. The problems of proton dynamics on tautomeric O–H···O bonds are considered. A brief description of the N–H···O and C–H···Y hydrogen bonds is given.
MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients
Authors: Chu Pei-Yi , Wu Hsing-Ju , Wang Shin-Mae , Chen Po-Ming , Tang Feng-Yao , Chiang En-Pei Isabel
Published: May 2021

(1) Background: methionine cycle is not only essential for cancer cell proliferation but is also critical for metabolic reprogramming, a cancer hallmark. Hepatic and extrahepatic tissues methionine adenosyltransferases (MATs) are products of two genes, MAT1A and MAT2A that catalyze the formation of S-adenosylmethionine (SAM), the principal biological methyl donor. Glycine N-methyltransferase (GNMT) further utilizes SAM for sarcosine formation, thus it regulates the ratio of SAM:S-adenosylhomocysteine (SAH). (2) Methods: by analyzing the TCGA/GTEx datasets available within GEPIA2, we discovered that breast cancer patients with higher MAT2A had worse survival rate (p = 0.0057). Protein expression pattern of MAT1AA, MAT2A and GNMT were investigated in the tissue microarray in our own cohort (n = 252) by immunohistochemistry. MAT2A C/N expression ratio and cell invasion activity were further investigated in a panel of breast cancer cell lines. (3) Results: GNMT and MAT1A were detected in the cytoplasm, whereas MAT2A showed both cytoplasmic and nuclear immunoreactivity. Neither GNMT nor MAT1A protein expression was associated with patient survival rate in our cohort. Kaplan–Meier survival curves showed that a higher cytoplasmic/nuclear (C/N) MAT2A protein expression ratio correlated with poor overall survival (5 year survival rate: 93.7% vs. 83.3%, C/N ratio ≥ 1.0 vs. C/N ratio < 1.0, log-rank p = 0.004). Accordingly, a MAT2A C/N expression ratio ≥ 1.0 was determined as an independent risk factor by Cox regression analysis (hazard ratio = 2.771, p = 0.018, n = 252). In vitro studies found that breast cancer cell lines with a higher MAT2A C/N ratio were more invasive. (4) Conclusions: the subcellular localization of MAT2A may affect its functions, and elevated MAT2A C/N ratio in breast cancer cells is associated with increased invasiveness. MAT2A C/N expression ratio determined by IHC staining could serve as a novel independent prognostic marker for breast cancer.
Deep Learning in Pancreatic Tissue: Identification of Anatomical Structures, Pancreatic Intraepithelial Neoplasia, and Ductal Adenocarcinoma
Authors: Kriegsmann Mark , Kriegsmann Katharina , Steinbuss Georg , Zgorzelski Christiane , Kraft Anne , Gaida Matthias M.
Published: May 2021

Identification of pancreatic ductal adenocarcinoma (PDAC) and precursor lesions in histological tissue slides can be challenging and elaborate, especially due to tumor heterogeneity. Thus, supportive tools for the identification of anatomical and pathological tissue structures are desired. Deep learning methods recently emerged, which classify histological structures into image categories with high accuracy. However, to date, only a limited number of classes and patients have been included in histopathological studies. In this study, scanned histopathological tissue slides from tissue microarrays of PDAC patients (n = 201, image patches n = 81.165) were extracted and assigned to a training, validation, and test set. With these patches, we implemented a convolutional neuronal network, established quality control measures and a method to interpret the model, and implemented a workflow for whole tissue slides. An optimized EfficientNet algorithm achieved high accuracies that allowed automatically localizing and quantifying tissue categories including pancreatic intraepithelial neoplasia and PDAC in whole tissue slides. SmoothGrad heatmaps allowed explaining image classification results. This is the first study that utilizes deep learning for automatic identification of different anatomical tissue structures and diseases on histopathological images of pancreatic tissue specimens. The proposed approach is a valuable tool to support routine diagnostic review and pancreatic cancer research.