bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of dietary pattern on anti-tuberculosis treatment outcomes in persons with dysglycemia: a Peruvian prospective cohort study
Authors: Arriaga María B. , Araújo-Pereira Mariana , Andrade Vanessa M. B. , Queiroz Artur T. L. , Fernandes Catarina D. , Sales Caio , Aliaga Juan Gonzalo , Shivakoti Rupak , Lecca Leonid , Calderon Roger I. , Andrade Bruno B.
Published: December 2023

Introduction

Dietary patterns (DPs) are associated with overall nutritional status and may alter the clinical prognosis of tuberculosis. This interaction can be further intricated by dysglycemia (i.e., diabetes or prediabetes). Here, we identified DPs that are more common with tuberculosis–dysglycemia and depicted their association with tuberculosis treatment outcomes.

Methods

A prospective cohort study of persons with tuberculosis and their contacts was conducted in Peru. A food frequency questionnaire and a multidimensional systems biology-based analytical approach were employed to identify DPs associated with these clinical groups. Potential independent associations between clinical features and DPs were analyzed.

Results

Three major DPs were identified. TB–dysglycemia cases more often had a high intake of carbohydrates (DP1). Furthermore, DP1 was found to be associated with an increased risk of unfavorable TB outcomes independent of other factors, including dysglycemia.

Conclusion

Our findings suggest that the evaluation of nutritional status through DPs in comorbidities such as dysglycemia is a fundamental action to predict TB treatment outcomes. The mechanisms underlying the association between high intake of carbohydrates, dysglycemia, and unfavorable tuberculosis treatment outcomes warrant further investigation.
Opioid, methamphetamine, and polysubstance use: perinatal outcomes for the mother and infant
Authors: Wouldes Trecia A. , Lester Barry M.
Published: December 2023

The escalation in opioid pain relief (OPR) medications, heroin and fentanyl, has led to an increased use during pregnancy and a public health crisis. Methamphetamine use in women of childbearing age has now eclipsed the use of cocaine and other stimulants globally. Recent reports have shown increases in methamphetamine are selective to opioid use, particularly in rural regions in the US. This report compares the extent of our knowledge of the perinatal outcomes of OPRs, heroin, fentanyl, two long-acting substances used in the treatment of opioid use disorders (buprenorphine and methadone), and methamphetamine. The methodological limitations of the current research are examined, and two important initiatives that will address these limitations are reviewed. Current knowledge of the perinatal effects of short-acting opioids, OPRs, heroin, and fentanyl, is scarce. Most of what we know about the perinatal effects of opioids comes from research on the long-acting opioid agonist drugs used in the treatment of OUDs, methadone and buprenorphine. Both have better perinatal outcomes for the mother and newborn than heroin, but the uptake of these opioid substitution programs is poor (<50%). Current research on perinatal outcomes of methamphetamine is limited to retrospective epidemiological studies, chart reviews, one study from a treatment center in Hawaii, and the US and NZ cross-cultural infant Development, Environment And Lifestyle IDEAL studies. Characteristics of pregnant individuals in both opioid and MA studies were associated with poor maternal health, higher rates of mental illness, trauma, and poverty. Infant outcomes that differed between opioid and MA exposure included variations in neurobehavior at birth which could complicate the diagnosis and treatment of neonatal opioid withdrawal (NOWs). Given the complexity of OUDs in pregnant individuals and the increasing co-use of these opioids with MA, large studies are needed. These studies need to address the many confounders to perinatal outcomes and employ neurodevelopmental markers at birth that can help predict long-term neurodevelopmental outcomes. Two US initiatives that can provide critical research and treatment answers to this public health crisis are the US Environmental influences on Child Health Outcomes (ECHO) program and the Medication for Opioid Use Disorder During Pregnancy Network (MAT-LINK).
Adding the latency period to a muscle contraction model coupled to a membrane action potential model
Authors: Kappaun Nadia Roberta Chaves , Graça Ana Beatriz Nogueira Rubião , Lavinas Gonçalves Gabriel Benazzi , Weber dos Santos Rodrigo , Vecchio Sara Del , Bastos Flávia Souza
Published: December 2023

Introduction: Skeletal muscle is responsible for multiple functions for maintaining energy homeostasis and daily activities. Muscle contraction is activated by nerve signals, causing calcium release and interaction with myofibrils. It is important to understand muscle behavior and its impact on medical conditions, like in the presence of some diseases and their treatment, such as cancer, which can affect muscle architecture, leading to deficits in its function. For instance, it is known that radiotherapy and chemotherapy also have effects on healthy tissues, leading to a reduction in the rate of force development and the atrophy of muscle fibers. The main aim is to reproduce the behavior of muscle contraction using a coupled model of force generation and the action potential of the cell membrane, inserting the latency period observed between action potential and force generation in the motor unit.

Methods: Mathematical models for calcium dynamics and muscle contraction are described, incorporating the role of calcium ions and rates of reaction. An action potential initiates muscle contraction, as described by the Hodgkin–Huxley model. The numerical method used to solve the equations is the forward Euler method.

Results and Discussion: The results show dynamic calcium release and force generation, aligning with previous research results, and the time interval between membrane excitation and force generation was accomplished. Future work should suggest simulating more motor units at the actual scale for the possibility of a comparison with real data collected from both healthy individuals and those who have undergone cancer treatment.
In vitro application of Eruca vesicaria subsp. sativa leaf extracts and associated metabolites reduces the growth of Oomycota species involved in Kiwifruit Vine Decline Syndrome
Authors: Mian Giovanni , Zuiderduin Kathryn , Barnes Luke S. , Loketsatian Supasan , Bell Luke , Ermacora Paolo , Cipriani Guido
Published: December 2023

This study aimed to determine whether leaf extracts from seven Eruca vesicaria subsp. sativa cultivars and their biochemically active compounds (glucosinolates and downstream-derived products) inhibit mycelia growth of three well-known pathogenic oomycetes, Phytopythium chamaehyphon, Phytopythium vexans and Phytophthora citrophthora; being the most significant in the development of Kiwifruit Vine Decline Syndrome (KVDS). Leaf extract quantity of 10, 20 and 30 mg were inoculated in Petri dish (90 mm Ø, each 22 mL of liquid medium – Potato Dextrose Agar), for in vitro bioassays. A pathogen plug was placed in the centre of each plate and the Oomycota colony perimeter was marked 5 days after inoculation. Radial colony growth was measured from 4 marks per plate 5, 10, and 15 days after inoculation, further elaborated with Image J software image analysis. Growth rates for all strains were inhibited by around 67% after 15 days. This was most pronounced when applying the highest concentration of leaf extract. By using Liquid Chromatography Mass Spectrometry (LC-MS) and Gas Chromatography Mass Spectrometry (GC-MS), fifteen glucosinolate compounds, of which glucosativin was found in the highest quantity, were identified. Concentrations of hydrolysis products produced by leaves (erucin and sativin) were also investigated, and were significantly associated with colony radial growth, especially towards Pp. chamaehyphon and Pp. vexans. Three downstream products of glucosinolates (two pure isothiocyanates, AITC and PEITC; and one indole I3C; all commonly present in Brassicaceae) were also tested, and a statistically significant inhibition of growth was observed at the highest concentration (0.6 µL).
Decision-tree-based ion-specific dosing algorithm for enhancing closed hydroponic efficiency and reducing carbon emissions
Authors: Cho Woo-Jae , Gang Min-Seok , Kim Dong-Wook , Kim JooShin , Jung Dae-Hyun , Kim Hak-Jin
Published: December 2023

The maintenance of ion balance in closed hydroponic solutions is essential to improve the crop quality and recycling efficiency of nutrient solutions. However, the absence of robust ion sensors for key ions such as P and Mg and the coupling of ions in fertilizer salts render it difficult to effectively manage ion-specific nutrient solutions. Although ion-specific dosing algorithms have been established, their effectiveness has been inadequately explored. In this study, a decision-tree-based dosing algorithm was developed to calculate the optimal volumes of individual nutrient stock solutions to be supplied for five major nutrient ions, i.e., NO3, K, Ca, P, and Mg, based on the concentrations of NO3, K, and Ca and remaining volume of the recycled nutrient solution. In the performance assessment based on five nutrient solution samples encompassing the typical concentration ranges for leafy vegetable cultivation, the ion-selective electrode array demonstrated feasible accuracies, with root mean square errors of 29.5, 10.1, and 6.1 mg·L-1 for NO3, K, and Ca, respectively. In a five-step replenishment test involving varying target concentrations and nutrient solution volumes, the system formulated nutrient solutions according to the specified targets, exhibiting average relative errors of 10.6 ± 8.0%, 7.9 ± 2.1%, 8.0 ± 11.0%, and 4.2 ± 3.7% for the Ca, K, and NO3 concentrations and volume of the nutrient solution, respectively. Furthermore, the decision tree method helped reduce the total fertilizer injections and carbon emissions by 12.8% and 20.6% in the stepwise test, respectively. The findings demonstrate that the decision-tree-based dosing algorithm not only enables more efficient reuse of nutrient solution compared to the existing simplex method but also confirms the potential for reducing carbon emissions, indicating the possibility of sustainable agricultural development.
Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN in Corymbia citriodora
Authors: Wang Chubiao , Luo Jianzhong , He Wenliang , Huang Anying , Lu Wanhong , Lin Yan , Ou Yuduan
Published: December 2023

The GDP-D-mannose pyrophosphorylase (GMP) and microtubule severing enzyme KATANIN (KTN) are crucial for wood formation. Although functional identification has been performed in Arabidopsis, few comprehensive studies have been conducted in forest trees. In this study, we discovered 8 CcGMP and 4 CcKTN genes by analyzing the whole genome sequence of Corymbia citriodora. The chromosomal location, genome synteny, phylogenetic relationship, protein domain, motif identification, gene structure, cis-acting regulatory elements, and protein-interaction of CcGMP and CcKTN were all investigated. KTN has just one pair of segmentally duplicated genes, while GMP has no duplication events. According to gene structure, two 5’ UTRs were identified in CcGMP4. Furthermore, there is no protein-interaction between KTN and GMP. Based on real-time PCR, the expression of most genes showed a positive connection with DBH diameters. In addition, the expression of CcGMP4 and CcKTN4 genes were greater in different size tree, indicating that these genes are important in secondary xylem production. Overall, this findings will enhance our comprehension of the intricacy of CcGMP&CcKTN across diverse DBHs and furnish valuable insights for future functional characterization of specific genes in C. citriodora.
Effects of mindfulness-based cognitive therapy on older adults with sleep disorders: a systematic review and meta-analysis
Authors: González-Martín Ana María , Aibar-Almazán Agustín , Rivas-Campo Yulieth , Marín-Gutiérrez Alejandro , Castellote-Caballero Yolanda
Published: December 2023

Objective

This systematic review and meta-analysis was conducted to analyze the effectiveness of a mindfulness-based program on sleep quality in healthy non-institutionalized older people.

Methods

This study was conducted following the PRISMA (Preferred Reporting Items For Systematic Reviews And Meta-Analyses) guidelines. The search was conducted during May and June 2023 using four databases: Pubmed, Scopus, Web of Science, and CINAHL. Different keywords combined with Boolean operators were used. Only 10 articles of the initial 177 were included. In the study, the standardized mean difference (SMD) was used along with a 95% confidence interval to measure the effect. Heterogeneity among the studies, assessed using Cochran's Q-test and the I2 statistic was found to be low, leading to the use of a fixed-effects model in the analysis. The effect size was expressed as Hedge'g. Furthermore, a subgroup analysis was conducted, taking into account the various tools used to assess sleep conditions.

Results

Mindfulness was found to reduce poor sleep quality in people with both long-term and short-term sleep disorders. Weighting effect model Hedge'g = −0.344 with a 95% confidence interval ranging from −0.425 to −0.263. In all cases, statistically significant results were observed, as well as moderate and negative effect sizes according to the Hedge's g index: −0.326 for Insomnia Severity Index (ISI), −0.343 for Pittsburgh Sleep Quality Index (PSQI), and −0.28 for Sleep Onset Latency (SOL).

Conclusion

This systematic review and meta-analysis found that mindfulness can be used to remedy poor sleep quality in older people, so it could be a viable treatment option for insomnia or other problems related to poor sleep quality in this population.
The impact of primary care access on autism spectrum disorder awareness in an underserved population
Authors: O’Donnell Mackenzie T. , Schmidt Randall B. , Butler Faith M.
Published: December 2023

American Academy of Pediatrics (AAP) recommendations for Autism Spectrum Disorder (ASD) screening do not specifically address safety-net clinics, which provide multidisciplinary healthcare services to underserved patients. This project explored the potential for ASD screening in safety-net clinics by assessing parental perceived knowledge of ASD at JayDoc Free Clinic, a student-run safety-net clinic in Wyandotte County, Kansas. May through December 2022, patients who reported to be the parent of a minor received a demographic survey and a Likert-style questionnaire assessing perceived knowledge of ASD, including understanding the importance of ASD screening and ASD signs and symptoms. Responses were categorized into positive, negative, and unsure. Demographic variables included the minor’s primary care provider (PCP) status. Results were analyzed using bivariate analysis, with chi-square tests for significance (p-value ≤ 0.05). Of the 52 participants who completed at least one Likert response, 55.8% reported their child had a PCP. Responses were somewhat balanced with 44.2% positive for understanding the importance of ASD screening and 53.8% positive for understanding ASD signs and symptoms. For understanding the signs and symptoms of ASD, an unsure response (32.7% of responses) was statistically associated with a lack of PCP (p = 0.017). The balance of positive with negative and unsure responses could reflect lack of ASD knowledge and may relate to healthcare inaccessibility. This is consistent with the significant association between lack of PCP and unsure responses for understanding ASD signs and symptoms. ASD screening and education in safety-net clinics like JayDoc could be valuable, particularly for children without a PCP.
Trends in outpatient and inpatient visits for separate ambulatory-care-sensitive conditions during the first year of the COVID-19 pandemic: a province-based study
Authors: Kendzerska Tetyana , Zhu David T. , Pugliese Michael , Manuel Douglas , Sadatsafavi Mohsen , Povitz Marcus , Stukel Therese A. , To Teresa , Aaron Shawn D. , Mulpuru Sunita , Chin Melanie , Kendall Claire E. , Thavorn Kednapa , Robillard Rebecca , Gershon Andrea S.
Published: December 2023

Background

The COVID-19 pandemic led to global disruptions in non-urgent health services, affecting health outcomes of individuals with ambulatory-care-sensitive conditions (ACSCs).

Methods

We conducted a province-based study using Ontario health administrative data (Canada) to determine trends in outpatient visits and hospitalization rates (per 100,000 people) in the general adult population for seven ACSCs during the first pandemic year (March 2020–March 2021) compared to previous years (2016–2019), and how disruption in outpatient visits related to acute care use. ACSCs considered were chronic obstructive pulmonary disease (COPD), asthma, angina, congestive heart failure (CHF), hypertension, diabetes, and epilepsy. We used time series auto-regressive integrated moving-average models to compare observed versus projected rates.

Results

Following an initial reduction (March–May 2020) in all types of visits, primary care outpatient visits (combined in-person and virtual) returned to pre-pandemic levels for asthma, angina, hypertension, and diabetes, remained below pre-pandemic levels for COPD, and rose above pre-pandemic levels for CHF (104.8 vs. 96.4, 95% CI: 89.4–104.0) and epilepsy (29.6 vs. 24.7, 95% CI: 22.1–27.5) by the end of the first pandemic year. Specialty visits returned to pre-pandemic levels for COPD, angina, CHF, hypertension, and diabetes, but remained above pre-pandemic levels for asthma (95.4 vs. 79.5, 95% CI: 70.7–89.5) and epilepsy (53.3 vs. 45.6, 95% CI: 41.2–50.5), by the end of the year. Virtual visit rates increased for all ACSCs. Among ACSCs, reductions in hospitalizations were most pronounced for COPD and asthma. CHF-related hospitalizations also decreased, albeit to a lesser extent. For angina, hypertension, diabetes, and epilepsy, hospitalization rates reduced initially, but returned to pre-pandemic levels by the end of the year.

Conclusion

This study demonstrated variation in outpatient visit trends for different ACSCs in the first pandemic year. No outpatient visit trends resulted in increased hospitalizations for any ACSC; however, reductions in rates of asthma, COPD, and CHF hospitalizations persisted.
BPSDiary study protocol: a multi-center randomized controlled trial to compare the efficacy of a BPSD diary vs. standard care in reducing caregiver's burden
Authors: Pozzi Federico Emanuele , Calì Luisa , D'Antonio Fabrizia , Altomare Arianna Ida , Sepe Monti Micaela , Panigutti Massimiliano , Di Crosta Adolfo , Palumbo Rocco , Bonanni Laura , Carlucci Valentina , Bussè Cinzia , Cagning Annachiara , Urso Daniele , Vilella Davide , Logroscino Giancarlo , Alberoni Margherita , Bellinvia Angelo , Farina Elisabetta , de Rino Francesca , Gavazzi Armando , Zuffi Marta , Bruno Giuseppe , Bessi Valentina , Cotta Ramusino Matteo , Perini Giulia , Costa Alfredo , Ferrarese Carlo , Appollonio Ildebrando , Tremolizzo Lucio
Published: December 2023

Behavioral and Psychological Symptoms of Dementia (BPSD) are a heterogeneous set of psychological and behavioral abnormalities seen in persons with dementia (PwD), significantly impacting their quality of life and that of their caregivers. Current assessment tools, such as the Neuropsychiatric Inventory (NPI), are limited by recall bias and lack of direct observation. This study aims to overcome this limitation by making caregiver reports more objective through the use of a novel instrument, referred to as the BPSDiary. This randomized controlled trial will involve 300 caregiver-PwD dyads. The objective is to evaluate whether the use of the BPSDiary could significantly reduce caregiver burden, assessed using the Zarit Burden Interview (ZBI), compared to usual care. The study will include adult PwD, caregivers living with or close to the patient, and BPSD related to the HIDA (hyperactivity, impulsivity, irritability, disinhibition, aggression, agitation) domain. Caregivers randomized to the intervention arm will use the BPSDiary to record specific BPSD, including insomnia, agitation/anxiety, aggression, purposeless motor behavior, and delusions/hallucinations, registering time of onset, severity, and potential triggers. The primary outcome will be the change in ZBI scores at 3 months, with secondary outcomes including changes in NPI scores, olanzapine equivalents, NPI-distress scores related to specific BPSD domains, and caregiver and physician satisfaction. The study will be conducted in 9 Italian centers, representing diverse geographic and sociocultural contexts. While potential limitations include the relatively short observation period and the focus on specific BPSD disturbances, the BPSDiary could provide physicians with objective data to tailor appropriate non-pharmacological and pharmacological interventions. Additionally, it may empower caregivers by encouraging reflection on BPSD triggers, with the potential to improve the quality of life for both PwD and their caregivers.

Trial registry

NCT05977855.
Exploring the high abundance discrepancy in the planetary nebula IC 4663
Authors: Mohery M. , Ali A. , Khames Aya A. , Snaid S. , Mindil A.
Published: December 2023

This article presents an in-depth analysis of integral field unit spectroscopy performed on the Galactic planetary nebula (PN) IC 4663, with a primary focus on investigating its physical characteristics, chemical composition, and morphological properties. The examination involved the utilization of a series of emission-line maps representing various elements and ionization zones, allowing for a comprehensive assessment of morphological and ionization structure of IC 4663. The majority of these maps depict an overall elliptical shape, featuring a relatively faint core at the center. Emanating from this core are two distinct and illuminated lobes extending in opposite directions, forming a conspicuous double-lobed configuration. The chemical analysis conducted on IC 4663 has revealed that the PN is rich in both helium and nitrogen, characterized by an N/O ratio exceeding 0.5, classifying it as a Peimbert type I nebula. Furthermore, the presence of a notably intense He II emission line at λ4686, in conjunction with the existence of high-excitation lines within the nebular spectrum, indicate its classification as a high-excitation class nebula. The computation of O2+, N2+, and N3+ through optical recombination lines has unveiled extreme abundance discrepancy factors, with values of 39.0 ± 6 for O2+ and 37.0 ± 9 for N2+ and N3+. These findings suggest the possibility that the central star of IC 4663 may be part of a binary system with a sub-stellar companion, potentially engaged in a common envelope interaction. Recent astrometric data derived from the Gaia mission further indicate a significant likelihood of interaction between the asymptotic giant branch halo of the PN and its surrounding interstellar medium, particularly in the southeast direction.
Attitudes to acute pain and the use of pain assessment scales among Spanish small animal veterinarians
Authors: Menéndez Sandra , Cabezas Miguel Angel , Gomez de Segura Ignacio A.
Published: December 2023

Evaluation of acute pain in dogs and cats is the basis for an appropriate treatment and improved well-being. The difficulties involved in pain assessment pose a challenge for veterinarians, and recent findings indicate that the most effective tools available—validated pain assessment scales—may not be widely utilized.

Objectives

This study aimed to characterize the level of concern among Spanish veterinarians regarding acute pain in companion animals. Additionally, it sought to determine whether this concern correlates with the utilization of optimal assessment tools.

Methods

A survey was conducted to explore Spanish veterinarians’ attitudes toward pain and its assessment. The survey was distributed through two most prominent small animal veterinary associations, the Spanish association for veterinary anesthesia and analgesia, as well as key industry players committed to proactive pain management. Descriptive analysis of the collected data was performed using Excel and SPSS.

Results

A total of 292 veterinarians participated in the study. A high level of concern regarding pain in dogs and cats was determined where 44% of surveyed veterinarians assessed pain in all patients. Despite an awareness of validated pain scales, only 28% used them. The preferred scales were the Glasgow CMPS for dogs (94%) and the Feline Grimace Scale for cats (93%). Among respondents who do not use these validated tools, there was a considerable interest in incorporating these scales into practice (85%) and considered lack of training was the most relevant issue (32%). Other challenges to scale utilization were identified, including constraints related to time, staffing, and the need to establish a habit.

Conclusions and relevance

Spanish small animal veterinarians demonstrated a strong awareness to pain in their patients and employed various methods for pain assessment. However, a limited use of validated tools was identified and likely attributed to challenges such as a lack of established routine, time constraints, insufficient personnel, and, notably, a knowledge gap among veterinarians who do not employ pain assessment scales. The most commonly used scales were the Glasgow CMPS for dogs and the FGS for cats. Overall, these results suggest a window of opportunity for the implementation of training programs in small animal pain assessment at a national level.
Amelogenesis imperfectában szenvedő páciens komplex protetikai ellátása digitális munkafolyamatokkal
Authors: Lipták Klaudia , Lipták Laura , Déri Tamás , Hermann Péter
Published: December 2023

Bevezetés: Az amelogenesis imperfecta (AI) olyan örökletes betegség, amely mennyiségi vagy minőségi fogzománchibákatmutat szisztémás megnyilvánulások hiányában. Az AI gyakran társul nyitott harapással, amely szintén komplexellátást igényel. A végleges fogászati kezelésben az indirekt pótlások készítésére kell összpontosítani, így a korai felismerésés ellátás a hosszú távú sikeresség kulcstényezői, mivel a betegségben érintett zománc esendőbb a fogszuvasodássalszemben.Esetismertetés: 15 éves fiatal nőbeteg 2022-ben jelentkezett a Semmelweis Egyetem Fogpótlástani Klinikáján az AIkövetkeztében erősen lekopott fogai miatt. Az intraorális vizsgálat során minden fogán redukált zománcot találtunk, illetvenyitott harapása is volt. A fogfejlődési rendellenességre vonatkozó aktuális szakmai ajánlásokat követve az alsó ésfelső állcsonton monolitikus cirkónium-dioxid koronák, sínek és a kialakult foghiányok ellátására hidak készítését terveztük,valamint harapásemelésre is szükség volt.Összefoglalás: Az AI-ban szenvedő betegek számára a fogak megjelenése pszichológiai és funkcionális aggodalmakraadhat okot, így minden kezelési terv célja a helyreállítás és a rendszeres nyomon követés. Az AI-s betegek eseténrendkívül fontos a megfelelő szájhigiéné fenntartása. Ilyen kórképnél az indirekt restaurátumokkal való ellátást preferáljaa szakirodalom.
Rainwater: Harvesting and Storage through a Flexible Storage System to Enhance Agricultural Resilience
Authors: Pari Luigi , Cozzolino Luca , Bergonzoli Simone
Published: December 2023

Many climatic variables are projected to occur with more intense and frequent extreme events, possibly unpredictable patterns and negative feedback loops with other environmental processes. Agriculture has faced uncertainty regarding ground temperature and rainfall distribution during the last few years, making water availability one of the major concerns for farm management. In this scenario, rainwater harvesting could represent a powerful tool to mitigate this problem, and consequently, the research community has been fostering new technical solutions. On the other hand, a few studies on agronomic assessment of rainwater harvesting systems are present in scientific literature. The present study reports preliminary data of a long-term study on a Flexible Water Storage System (FWSS) evaluating the possibility of enhancing agriculture systems resilience, shifting from rainfed production to irrigated agriculture relying on excessive rainfall, collectible from extreme events. The idea of intercepting excess rainfall, which is generally lost, thanks to an innovative water harvesting system, and using it to mitigate drought stress for crops is in line with sustainable approaches aiming to improve the resilience of agricultural systems. The results highlighted that the system studied could potentially collect an annual average of 831.7 m3 of water, mitigating the excess of water in the ditch that can potentially cause flooding and storing fresh water to provide irrigation during dry periods.
Responses of Crop and Soil Phosphorus Fractions to Long-Term Fertilization Regimes in a Loess Soil in Northwest China
Authors: Khan Asif , Yang Xueyun , Sun Benhua , Zhang Shulan , He Binghui
Published: December 2023

Contrasting fertilization modifies soil phosphorus (P) transformation and bioavailability, which impact crop P uptake and P migration in the soil profile. A long-term (25-year) fertilizer experiment was employed to investigate crop yield, P uptake and changes in sequentially extracted P fractions in the soil profile, and their relationships on a calcareous soil derived from loess material under a winter wheat and summer maize double-cropping system. The experiment involved seven nutrient management treatments: control (CK, no nutrient input), N, NK, NP, and NPK, representing various combinations of synthetic nitrogen (N), phosphate (P), and potassium (K) applications, as well as combinations of NPK fertilizers with either crop residues (SNPK, where S refers to maize stalk or wheat straw) or manure (MNPK, where M refers to dairy manure). Wheat and maize yields were significantly higher with P input fertilizer relative to the P-omitted treatments. Long-term application of P-containing fertilizers markedly raised the contents of inorganic (Pi) and organic (Po) P fractions at 0–20 cm depth compared with the P-omitted treatments. Moreover, both Pi and Po fractions were markedly higher under MNPK than under NPK and SNPK treatments. For achieving high yield for wheat and maize, the critical contents of labile P were 54 and 63 mg kg−1, and those of moderately labile P were 48 and 49 mg kg−1, respectively, defined by the linear plateau model. In addition, the change points of labile P and moderately labile P were 99 and 70 mg kg−1, above which CaCl2-P content significantly increased. Moreover, long-term P input significantly accumulated different P fractions in the deeper soil layers up to 100 cm, with large portions of organic P being a composite of labile and moderately labile P, especially in MNPK treatment. Our results suggest that excessive P supply with organic manure resulted in massive P accumulation in the topsoil and promoted soil P fraction transformation and availability in the deep soil layers, especially in an organic P form that has often been neglected.
Soil Salinity Mapping of Croplands in Arid Areas Based on the Soil–Land Inference Model
Authors: Gao Shenghan , Wang Xinjun , Xu Shixian , Su Tong , Yang Qiulan , Sheng Jiandong
Published: December 2023

Soil salinization can decrease soil productivity and is a significant factor in causing land degradation. Precision mapping of salinization in agricultural fields would improve farmland management. This study focuses on the cropland in the Manas River Basin, located in the arid region of northwest China. It explores the potential of a soil mapping method, the Soil–Land Inference Model (SoLIM), which only requires a small number of soil samples to infer soil salinization of farmlands in arid areas. The model was utilized to create spatial distribution maps of soil salinity for the years 2009 and 2017, and changes in the distribution were analyzed. The research results indicate: (1) Through the analysis of sample point data, it was observed that soil salinity in the study area tends to accumulate in the surface layer (0–30 cm) in spring and in the subsoil layer (60–90 cm) during the crop growing season, with significant spatial variability. Therefore, it is necessary to conduct detailed salinity mapping. (2) Using field measurements as validation data, the simulation results of the SoLIM were compared with spatial interpolation methods and regression models. The SoLIM showed higher inference accuracy, with R2 values for the simulation results of the three soil layers all exceeding 0.5. (3) The SoLIM spatial inference showed salt accumulation in the northern part and desalination in the southern part. The findings of this study suggest that the SoLIM has the potential to effectively map soil salinization of croplands in arid areas, offering an efficient solution for monitoring soil salinity in arid oasis croplands.
Effectiveness of Oregano and Thyme Essential Oils as Alternatives for Sulfur Dioxide in Controlling Decay and Gray Mold and Maintaining Quality of ‘Flame Seedless’ Table Grape (Vitis vinifera L.) during Cold Storage
Authors: El-Abbasy Usama K. , Abdel-Hameed Mohamed A. , Hatterman-Valenti Harlene M. , El-Shereif Ali R. , Abd El-Khalek Ahmed F.
Published: December 2023

The current study was carried out over two seasons (2020 and 2021) to assess the effects of preharvest treatments with oregano and thyme essential oils (EOs) as an alternative to the traditional use of sulfur dioxide (SO2) during cold storage of grape clusters cv. Flame Seedless. Grapevines were sprayed with oregano or thyme essential oils at 2000 or 4000 µL/L two days before harvest. The results confirmed that oregano and thyme EOs treatments reduced the physiological loss in weight, decay incidence, gray mold, rachis browning index, and berry shattering as compared to sulfur dioxide and untreated fruits. In addition, EOs had higher marketable percentage, firmness, and visual appearance cluster scores, while they reduced the deterioration in titratable acidity (TA) and ascorbic acid (AsA) contents, slowing the increases in soluble solids content (SSC) and SSC/TA ratio of berries, and improving total anthocyanin content. Moreover, these EOs delayed berry activities of polyphenol oxidase (PPO), peroxidase (POX), and pectin methylesterase (PME) enzymes during cold storage. Results suggest that preharvest application with either oregano or thyme EOs at 2000 µL/L might be a promising eco-friendly and safe candidate as an alternative to conventional SO2 used to control decay incidence and gray mold rot caused by Botrytis cinerea, and the EOs were effective in maintaining the quality of grape clusters during cold storage for up to 45 days.
Exogenous GR24 Inhibits Strawberry Tillering by Affecting the Phytohormone Signaling and Sugar Metabolism Pathways
Authors: Peng Yuting , Jiang Yuyan , He Caixia , She Musha , Li Mengyao , Chen Qing , Zhang Yong , Lin Yuanxiu , Zhang Yunting , Wang Yan , He Wen , Wang Xiaorong , Tang Haoru , Luo Ya
Published: December 2023

Tillering is an important part in strawberry growth, and strawberries can reproduce nutritionally through stolons to generate genetically stable offspring. However, excessive tillering during the fruit-growing stage can negatively impact fruit yield and quality. In this study, different concentrations of exogenous rac-GR24 (GR24) are used to treat the strawberry plants. It was found that GR24 effectively inhibited the sprouting of strawberry stolons, while promoting the growth of the stems and leaves. Among the treatments, the most effective concentration was found to be 5 μmol/L GR24. This treatment resulted in a decrease in the glucose content in the strawberry crowns and also caused changes in the contents of two endogenous phytohormones, gibberellic acid (GA3) and trans-zeatin riboside (tZR). Transcriptome data further suggested that exogenous GR24 may inhibit strawberry plant tillering by affecting various phytohormone signaling pathways and the sugar metabolism pathway. In 5 μmol/L GR24-treated plants, the expression level of type-B response regulator (B-ARR) was down-regulated and the expression level of CYTOKININ RESPONSE 1 (CRE1), histidine-containing phosphotransfer protein (AHP), and type-A response regulator (A-ARR) were up-regulated, suggesting the inhibition of the cytokinin (CTK) signaling pathway. The down-regulation of auxin (AUX) and auxin response factor (ARF), as well as the up-regulation of auxin/indole-3-acetic acid (AUX/IAA), led to the inhibition of the indole-3-acetic acid (IAA) signaling pathway. Additionally, the up-regulation of pyrabactin resistance 1/ pyrabactin resistance 1-like (PYR/PYL), non-fermenting 1-related protein kinase 2 (SnRK2), and ABRE binding factors (ABF) and the down-regulation of protein phosphatase 2C (PP2C) were observed in the up-regulated abscisic acid (ABA) signaling pathways. In the sugar metabolism pathway, the up-regulation of invertase (INV), hexokinase (HK), and fructokinase (FRK) and the down-regulation of trehalase (TREH) and beta-amylase (BMY) led to a decreased glucose synthesis and an increased glucose consumption. Therefore, GR24 can effectively inhibit strawberry plant tillering through these pathways, making it an effective reagent for tillering inhibition.
Estimation of Evapotranspiration from the People’s Victory Irrigation District Based on the Data Mining Sharpener Model
Authors: Zhang Jie , Li Shenglin , Wang Jinglei , Chen Zhifang
Published: December 2023

Reasonable evaluation of evapotranspiration (ET) is crucial for optimizing agricultural water resource management. In the study, we utilized the Data Mining Sharpener (DMS) model; the Landsat thermal infrared images were sharpened from a spatial resolution of 100 m to 30 m. We then used the Surface Energy Balance System (SEBS) to estimate daily ET during the winter wheat growing season in the People’s Victory Irrigation District in Henan, China. It was concluded that the spatiotemporal patterns of land surface temperature and daily evapotranspiration remained consistent before and after sharpening. Results showed that the R2 value between the ET of 30 m spatial resolution and the value by eddy covariance method reached 0.814, with an RMSE of 0.516 mm and an MAE of 0.245 mm. All of these were higher than those of 100 m spatial resolution (R2 was 0.802, the RMSE was 0.534 mm, and the MAE was 0.253 mm). Furthermore, the daily ET image with a 30 m spatial resolution exhibited clear texture and distinct boundaries, without any noticeable mosaic effects. The changes in surface temperature and ET were more consistent in complex subsurface environments. The daily evapotranspiration of winter wheat was significantly higher in areas with intricate drainage systems compared to other regions. During the early growth stage, daily evapotranspiration decreased steadily until the overwintering stage. After the greening and jointing stages, it began to increase and peaked during the sizing period. The correlation between net solar radiation and temperature with ET was significant, while relative humidity and soil moisture were negatively correlated with ET. Throughout the growth period, net solar radiation had the greatest effect on ET.
Increased Accuracy of Genomic Prediction Using Preselected SNPs from GWAS with Imputed Whole-Genome Sequence Data in Pigs
Authors: Liu Yiyi , Zhang Yuling , Zhou Fuchen , Yao Zekai , Zhan Yuexin , Fan Zhenfei , Meng Xianglun , Zhang Zebin , Liu Langqing , Yang Jie , Wu Zhenfang , Cai Gengyuan , Zheng Enqin
Published: December 2023

Enhancing the accuracy of genomic prediction is a key goal in genomic selection (GS) research. Integrating prior biological information into GS methods using appropriate models can improve prediction accuracy for complex traits. Genome-wide association study (GWAS) is widely utilized to identify potential candidate loci associated with complex traits in livestock and poultry, offering essential genomic insights. In this study, a GWAS was conducted on 685 Duroc × Landrace × Yorkshire (DLY) pigs to extract significant single-nucleotide polymorphisms (SNPs) as genomic features. We compared two GS models, genomic best linear unbiased prediction (GBLUP) and genomic feature BLUP (GFBLUP), by using imputed whole-genome sequencing (WGS) data on 651 Yorkshire pigs. The results revealed that the GBLUP model achieved prediction accuracies of 0.499 for backfat thickness (BFT) and 0.423 for loin muscle area (LMA). By applying the GFBLUP model with GWAS-based SNP preselection, the average prediction accuracies for BFT and LMA traits reached 0.491 and 0.440, respectively. Specifically, the GFBLUP model displayed a 4.8% enhancement in predicting LMA compared to the GBLUP model. These findings suggest that, in certain scenarios, the GFBLUP model may offer superior genomic prediction accuracy when compared to the GBLUP model, underscoring the potential value of incorporating genomic features to refine GS models.
Evaluation of Genetic Diversity in Quill Mites of the Genus Syringophiloidus Kethley, 1970 (Prostigmata: Syringophilidae) with Six New-to-Science Species
Authors: Glowska Eliza , Laniecka Izabella , Ostrowska Kamila , Gebhard Christina A. , Olechnowicz Julia , Dabert Miroslawa
Published: December 2023

Quill mites (Acariformes: Syringophilidae) are poorly explored bird parasites. Syringophiloidus Kethley, 1970, is the most specious and widespread genus in this family. It is believed to contain mono-, steno- and poly-xenous parasites and thus seems to be an exemplary for studies on biodiversity and host associations. In this work, we applied the DNA barcode marker (mitochondrial cytochrome c oxidase subunit I gene fragment, COI) to analyze the species composition and host specificity of representatives of fifteen Syringophiloidus populations parasitizing fifteen bird species. The neighbor joining analyses distinguished thirteen monophyletic lineages, almost completely corresponding to seven previously known species recognized based on morphological features, and six new-to-science species. The only exception is S. amazilia Skoracki, 2017, which is most likely conspecific with Syringophiloidus stawarczyki Skoracki, 2004. The intraspecific distances of all species were not higher than 0.9%, whilst the interspecific diversity ranged from 5.9% to 19.2% and 6.3–22.4%, inferred as the distances p and K2P, respectively. Although all putative species (except S. amazilia) are highly supported, the relationships between them have not been fully resolved and only faintly indicate that both host phylogeny and distributions influence the phylogenetic structure of quill mite taxa.
Comparative Assessment of Oxidative and Antioxidant Parameters in Mule and Horse Neonates during Their First Month of Extrauterine Adaptation
Authors: Riccio Amanda Vallone , Costa Barbara Kolecha , Alonso Maria Augusta , Affonso Fernanda Jordão , França Danilo Souza , Nichi Marcilio , Belli Carla Bargi , McLean Amy Katherine , Boakari Yatta Linhares , Fernandes Claudia Barbosa
Published: December 2023

After parturition, a rapid transition occurs from the intrauterine to the extrauterine milieu, exposing neonates to physiological circumstances characterized by oxidative conditions that instigate the generation of reactive oxygen species. These free radicals play pivotal roles in physiological processes; however, an imbalance between their production and the removal of antioxidants can result in severe cellular damage. The main objective of this study was to compare the oxidative and antioxidant profiles in mule and horse neonates immediately post-parturition, as well as at subsequent time points (1, 6, 12, and 24 h, 7 and 30 days) during their extrauterine existence. The parameters assessed included the systemic concentrations of Thiobarbituric Acid Reactive Substances (TBARS) and carbonyl groups; the activities of the antioxidants superoxide dismutase (SOD) and glutathione peroxidase (GPx); and the levels of the total, indirect, and direct bilirubin. Our results showed no interaction effect between the neonatal groups and the assessed time points for the variables under investigation. Notably, the concentrations of TBARS, as a marker of lipid peroxidation, and bilirubin were consistently lower in the mules, whereas the glutathione peroxidase (GPx) activity exhibited higher levels in this group. The bilirubin levels were notably reduced in the mule neonates. The TBARS demonstrated a progressive decrease over the observation period in both groups, while the GPx activity remained relatively stable from birth to 7 days, with a substantial increase evident at the 30-day mark. Protein oxidation was not affected by the group and time, while for the SOD values, all times were statistically similar, except for the lower activity at T1h. Consequently, our findings lead us to the conclusion that neonatal mules and horses manifest distinct patterns of oxidative activity and antioxidant capacity during the initial month of their extrauterine existence, potentially indicative of different adaptation mechanisms to the extrauterine environment.
C-Type Natriuretic Peptide Pre-Treatment Improves Maturation Rate of Goat Oocytes by Maintaining Transzonal Projections, Spindle Morphology, and Mitochondrial Function
Authors: Xu Rui , Pan Menghao , Yin Lu , Zhang Yiqian , Tang Yaju , Lu Sihai , Gao Yan , Wei Qiang , Han Bin , Ma Baohua
Published: December 2023

C-type natriuretic peptide (CNP) is a peptide molecule naturally found in follicles and can be used to extend meiotic resumption and enhance the potential for oocytes to develop. However, the mechanism by which CNP improves goat oocyte quality remains unclear. In this study, cumulus–oocyte complexes (COCs) from goats were pre-treated with CNP prior to IVM, and the results showed that pre-treatment with CNP enhanced goat oocyte maturation. First, we discovered that CNP maintained communication between cumulus cells and oocytes by regulating the transzonal projections (TZPs). We then found that CNP treatment reduced abnormal spindle formation and increased the expression of genes associated with spindle assembly and the spindle assembly checkpoint. Moreover, further analysis showed that oocytes exhibited better antioxidant ability in the CNP treatment group, which mainly manifested in higher glutathione (GSH) and lower reactive oxygen species (ROS) concentrations. Enhanced mitochondrial activity was signified via the augmented expression of mitochondrial oxidative metabolism and fusion and fission-related genes, thus diminishing the apoptosis of the oocytes. Overall, these results provide novel insights into the potential mechanism by which CNP treatment before IVM can improve oocyte quality.
Effect of an Alliaceae Encapsulated Extract on Growth Performance, Gut Health, and Intestinal Microbiota in Broiler Chickens Challenged with Eimeria spp.
Authors: Villar-Patiño Gonzalo , Camacho-Rea María del Carmen , Olvera-García Myrna Elena , Baltazar-Vázquez Julio César , Gómez-Verduzco Gabriela , Téllez Guillermo , Labastida Aurora , Ramírez-Pérez Aurora Hilda
Published: December 2023

This study analyzed the effects of an Alliaceae encapsulated extract (AE-e) on daily gain (ADG), feed intake (ADFI), feed conversion ratio (FCR), oocysts per gram of feces (OPG), intestinal lesion (LS), and microbiota composition in broilers challenged with Eimeria spp. A total of 4800 one day Cobb-500 were allotted into 10 treatment groups with 12 replicates of 40 birds in a 2 × 4 + 2 factorial arrangement. The first factor was non-challenged (NC) or challenged (C), the second was four levels of AE-e added in the basal diet, 0 (AE0), 250 (AE250), 500 (AE500), and 750 mg·kg−1 (AE750), plus two ionophore controls, non-challenged (NC-Ion) and challenged (C-Ion). No interactions were observed between factors (NC0, NC250, NC500, NC750, C0, C250, C500, and C750), while C-Ion improved FCR at 21 d. The challenge affected negatively ADG and FCR and promoted enteropathogens in cecum. AE750 improved FCR in the finisher and cumulative phases, while C-Ion had fewer total OPG than C0 and C250. Likewise, at 21d, C250, C500, and C-Ion had fewer LS than C0, while at 28 d, C750 showed lower than C-Ion. In the cecum microbiota, C500 had more Ruminococcus, Firmicutes b, and Intestinimonas than C-Ion. In summary, AE-e showed beneficial results in broilers infected with Eimeria spp.