bluuebunny/crossref_metadata_2025 huggingface dataset:
iPSCs derived from infertile men carrying complex genetic abnormalities can generate primordial germ-like cells
Authors: Mouka Aurélie , Arkoun Brahim , Moison Pauline , Drévillon Loïc , Jarray Rafika , Brisset Sophie , Mayeur Anne , Bouligand Jérôme , Boland-Auge Anne , Deleuze Jean-François , Yates Frank , Lemonnier Thomas , Callier Patrick , Duffourd Yannis , Nitschke Patrick , Ollivier Emmanuelle , Bourdin Arnaud , De Vos John , Livera Gabriel , Tachdjian Gérard , Maouche-Chrétien Leïla , Tosca Lucie
Published: August 2022

Despite increasing insight into the genetics of infertility, the developmental disease processes remain unclear due to the lack of adequate experimental models. The advent of induced pluripotent stem cell (iPSC) technology has provided a unique tool for in vitro disease modeling enabling major advances in our understanding of developmental disease processes. We report the full characterization of complex genetic abnormalities in two infertile patients with either azoospermia or XX male syndrome and we identify genes of potential interest implicated in their infertility. Using the erythroblasts of both patients, we generated primed iPSCs and converted them into a naive-like pluripotent state. Naive-iPSCs were then differentiated into primordial germ-like cells (PGC-LCs). The expression of early PGC marker genes SOX17, CD-38, NANOS3, c-KIT, TFAP2C, and D2-40, confirmed progression towards the early germline stage. Our results demonstrate that iPSCs from two infertile patients with significant genetic abnormalities are capable of efficient production of PGCs. Such in vitro model of infertility will certainly help identifying causative factors leading to early germ cells development failure and provide a valuable tool to explore novel therapeutic strategies.
A promising antimicrobial bionanocomposite based poly(3-hydroxybutyrate-co-3-hydroxyvalerate) reinforced silver doped zinc oxide nanoparticles
Authors: Ibrahim Mohammad I. , Alsafadi Diya , Alamry Khalid A. , Oves Mohammad , Alosaimi Abeer M. , Hussein Mahmoud A.
Published: August 2022

A bionanocomposite based on biosynthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and reinforced with silver@zinc oxide (Ag–ZnO) was synthesized in variable loadings of Ag–ZnO using the in-situ casting dissolution technique. The degradable biopolymer PHBV had been biosynthesized from date waste as a renewable carbon source. The fabricated products were investigated as promising antibacterial materials. The Ag–ZnO nanoparticles were also synthesized using the green method in the presence of Gum Arabic. The Ag–ZnO nanoparticles were loaded within the PHBV biopolymer backbone at concentration of 1%, 3%, 5% and 10%, PHBV/Ag–ZnO(1,3,5,10%). The chemical structure, morphology, physical and thermal properties of the PHBV/Ag–ZnO bionanocomposites were assessed via common characterization tools of FTIR, TGA, XRD, SEM and EDX. One step of the degradation process was observed in the range of 200–220 °C for all the obtained materials. The onset degradation temperature of the bionanocomposites have been noticeably increased with increasing the nanofiller loading percentage. In addition, fabricated products were investigated for their interesting antibacterial performance. A detailed biological screening for the obtained products was confirmed against some selected Gram-positive and Gram-negative strains S. aureus and E. coli, respectively. Overall, the bionanocomposite PHBV/Ag–ZnO(10%) was the most potent against both types of the selected bacteria. The order of bacterial growth inhibition on the surface of the fabricated bionanocomposites was detected as follows: PHBV/Ag–ZnO(10%) > PHBV/Ag–ZnO(5%) > PHBV/Ag–ZnO(3%) > PHBV/Ag–ZnO(1%).
3D geodynamic-geomorphologic modelling of deformation and exhumation at curved plate boundaries: Implications for the southern Alaskan plate corner
Authors: Koptev Alexander , Nettesheim Matthias , Falkowski Sarah , Ehlers Todd A.
Published: August 2022

Plate corners with extreme exhumation rates are important because they offer a perspective for understanding the interactions between tectonics and surface processes. The southern Alaskan margin with its curved convergent plate boundary and associated zones of localized uplift is a prime location to study active orogeny. Here, we present the results of fully-coupled thermo-mechanical (geodynamic) and geomorphologic numerical modelling, the design of which captures the key features of the studied area: subduction of oceanic lithosphere (Pacific plate) is adjacent to a pronounced asymmetric indenter dipping at a shallow angle (Yakutat microplate), which in turn is bounded to the east by a dextral strike-slip shear zone (Fairweather fault). The resulting first-order deformation/rock uplift patterns show strong similarities with observations. In particular, relatively young thermochronological ages are reproduced along the plate-bounding (Fairweather) transform fault and in the area of its transition to convergence (the St. Elias syntaxis). The focused exhumation of the Chugach Core also finds its equivalent in model predicted zones of high rock uplift rates in an isolated region above the indenter. From these results, we suggest that the general exhumation patterns observed in southern Alaska are controlled by mutually reinforcing effects of tectonic deformation and surface erosion processes.
The compound losartan cream inhibits scar formation via TGF-β/Smad pathway
Authors: Zhao Wan-Yi , Zhang Li-Yun , Wang Zheng-Cai , Fang Qing-Qing , Wang Xiao-Feng , Du Yong-Zhong , Shi Bang-Hui , Lou Dong , Xuan Gui-Da , Tan Wei-Qiang
Published: August 2022

The role of angiotensin receptor blocker in wound healing and cutaneous fibrosis has become a hotspot in recent years. We have developed a losartan cream that is comparable to triamcinolone ointment in inhibiting scarring. Considering the effects of chitosan and asiaticoside on wound healing and scarring, we added them to the losartan cream this time and improved the formula, expecting to get a better anti-scarring effect. The effects of creams were investigated on mouse scar model with triamcinolone ointment, onion extract gel, and commercial asiaticoside cream set as positive controls. A preliminary exploration of the mechanism involved in TGF-β/Smad pathway was performed in vivo and in vitro. With all results of anti-scarring, the compound losartan cream (containing chitosan, asiaticoside, and losartan) shows the best effect, followed by the chitosan asiaticoside cream. The treatment of the compound losartan cream inhibited expression of TGF-β1, collagen, and Smads, and decreased phosphorylation of Smad in vivo. These inhibitory effects were also confirmed in vitro. Our findings indicated that the compound losartan cream could inhibit scarring via TGF-β/Smad pathway. This cream might be an effective option for scar treatment.
Saliva microbiome, dietary, and genetic markers are associated with suicidal ideation in university students
Authors: Ahrens Angelica P. , Sanchez-Padilla Diego E. , Drew Jennifer C. , Oli Monika W. , Roesch Luiz F. W. , Triplett Eric W.
Published: August 2022

Here, salivary microbiota and major histocompatibility complex (MHC) human leukocyte antigen (HLA) alleles were compared between 47 (12.6%) young adults with recent suicidal ideation (SI) and 325 (87.4%) controls without recent SI. Several bacterial taxa were correlated with SI after controlling for sleep issues, diet, and genetics. Four MHC class II alleles were protective for SI including DRB1*04, which was absent in every subject with SI while present in 21.7% of controls. Increased incidence of SI was observed with four other MHC class II alleles and two MHC class I alleles. Associations between these HLA alleles and salivary bacteria were also identified. Furthermore, rs10437629, previously associated with attempted suicide, was correlated here with SI and the absence of Alloprevotella rava, a producer of an organic acid known to promote brain energy homeostasis. Hence, microbial-genetic associations may be important players in the diathesis-stress model for suicidal behaviors.
Pollen beetle offspring is more parasitized under moderate nitrogen fertilization of oilseed rape due to more attractive volatile signal
Authors: Zolotarjova Valentina , Remmel Triinu , Kännaste Astrid , Kaasik Riina , Niinemets Ülo , Veromann Eve
Published: August 2022

Biocontrol providing parasitoids can orientate according to volatile organic compounds (VOCs) of their host’s plants, the emission of which is potentially dependent on the availability of soil nitrogen (N). This paper aimed at finding the optimal N fertilization rate for oilseed rape (Brassica napus L.) to favor parasitism of pollen beetles (Brassicogethes aeneus Fab. syn. Meligethes aeneus Fab.) in a controlled environment. Pollen beetles preferred to oviposit into buds of plants growing under higher N fertilization, whereas their parasitoids favored moderate N fertilization. As a part of induced defense, the proportion of volatile products of glucosinolate pathway in the total oilseed rape VOC emission blend was increased. Our results suggest that the natural biological control of pollen beetle herbivory is best supported by moderate N fertilization rates.
Thin film flow and heat transfer of Cu-nanofluids with slip and convective boundary condition over a stretching sheet
Authors: Shahzad Azeem , Liaqat Fakhira , Ellahi Zaffer , Sohail Muhammad , Ayub Muhammad , Ali Mohamed R.
Published: August 2022

The flow and heat transfer in thin film of Cu-nanofluid over a stretching sheet by considering different shape factors (platelets, blades, bricks, sphere and cylinder) along with slip and convective boundary conditions is investigated. The governing partial differential equations are converted to nonlinear ordinary differential equations by means of suitable similarity transformation and then solved by using BVP4C in MATLAB. The physical significance of various parameters on velocity and temperature profiles are investigated and provided in the form of table and also presented graphically. It is noted that the Platelet-shaped nanoparticles has the highest heat transfer rate as compare to other particle’s shapes.
How people with disabilities influence crowd dynamics of pedestrian movement through bottlenecks
Authors: Geoerg Paul , Schumann Jette , Boltes Maik , Kinateder Max
Published: August 2022

Despite considerable research efforts, most controlled empirical studies on crowd movement usually rely on homogeneous crowds, i.e., research participants are typically young adults without disabilities. Consequently, relatively little is known about pedestrian movement in more diverse and heterogeneous crowd conditions, e.g., when persons with reduced mobility are present. This gap may be particularly relevant at bottlenecks, along the path of a moving crowd, that limit the capacity of pedestrian flow. Here, we present results from 12 studies in which participants (total N = 252) with and without visible disabilities moved together in a crowd. In each study, groups of participants walked together in a hallway with a bottleneck at the end. The point of speed adoption, distances between neighbours, and behavioural activities were analysed. We found (1) that participants with disabilities reduced their speed further away from the bottleneck than participants without disabilities; (2) participants without disabilities stayed closer to neighbors with disabilities than to neighbors without disabilities; and (3) participants interacted and communicated with each other to organise in front of the bottleneck. These results underline the importance of studying representative and heterogeneous samples in crowd dynamics. We also argue that more interdisciplinary research is needed to better understand the dynamics of interactions between neighbors in a crowd. A more nuanced understanding of pedestrian dynamics holds the promise of improving the validity of simulation tools such as movement and evacuation models.
Low muscle mass, low muscle function, and sarcopenia in the urban and rural elderly
Authors: Moon Sung Woo , Kim Kwang-Joon , Lee Han Sung , Yun Young Mi , Kim Jong-Eun , Chun You Jin , Kim Chang Oh
Published: August 2022

Health outcomes of the elderly vary between rural and urban areas. Sarcopenia is diagnosed as loss of muscle strength or impaired physical performance, namely “low muscle function” and low muscle mass. Outcomes of low muscle mass and low muscle function are not equal. This study aimed to investigate the prevalence of low muscle mass, low muscle function, and sarcopenia in rural and urban populations and to determine whether regional differences were associated with each of these components. Participants aged ≥ 69 years (n = 2354) were recruited from three urban districts and one rural district in Korea. Low muscle mass was defined by appendicular lean mass using bioelectrical impedance analysis. Low muscle function was defined by handgrip strength and 5-chair stand test. Sarcopenia was defined as low muscle mass plus low muscle function. The prevalence of low muscle function (53.7% vs. 72.8%), and sarcopenia (16.3% vs. 24.4%) were higher in the rural elderly population. Rural residence was associated with low muscle function (OR 1.63; 95% CI 1.13–2.37, P = 0.009), but not with low muscle mass (OR 0.58; 95% CI 0.22–1.54, P = 0.271) or with sarcopenia (OR 1.13; 95% CI 0.63–2.00, P = 0.683). Interventions to detect and improve low muscle function in rural elderly population are needed.
A novel differentiated HuH-7 cell model to examine bile acid metabolism, transport and cholestatic hepatotoxicity
Authors: Saran Chitra , Fu Dong , Ho Henry , Klein Abigail , Fallon John K. , Honkakoski Paavo , Brouwer Kim L. R.
Published: August 2022

Hepatic cell lines serve as economical and reproducible alternatives for primary human hepatocytes. However, the utility of hepatic cell lines to examine bile acid homeostasis and cholestatic toxicity is limited due to abnormal expression and function of bile acid-metabolizing enzymes, transporters, and the absence of canalicular formation. We discovered that culturing HuH-7 human hepatoma cells with dexamethasone (DEX) and 0.5% dimethyl sulfoxide (DMSO) for two weeks, with Matrigel overlay after one week, resulted in a shorter and improved differentiation process. These culture conditions increased the expression and function of the major bile acid uptake and efflux transporters, sodium taurocholate co-transporting polypeptide (NTCP) and the bile salt export pump (BSEP), respectively, in two-week cultures of HuH-7 cells. This in vitro model was further characterized for expression and function of bile acid-metabolizing enzymes, transporters, and cellular bile acids. Differentiated HuH-7 cells displayed a marked shift in bile acid composition and induction of cytochrome P450 (CYP) 7A1, CYP8B1, CYP3A4, and bile acid-CoA: amino acid N-acyltransferase (BAAT) mRNAs compared to control. Inhibition of taurocholate uptake and excretion after a 24-h treatment with prototypical cholestatic drugs suggests that differentiated HuH-7 cells are a suitable model to examine cholestatic hepatotoxicity.
RSPO3 is a novel contraction-inducible factor identified in an “in vitro exercise model” using primary human myotubes
Authors: Takahashi Tadahisa , Li Yuqing , Chen Weijian , Nyasha Mazvita R. , Ogawa Kazumi , Suzuki Kazuaki , Koide Masashi , Hagiwara Yoshihiro , Itoi Eiji , Aizawa Toshimi , Tsuchiya Masahiro , Suzuki Naoki , Aoki Masashi , Kanzaki Makoto
Published: August 2022

The physiological significance of skeletal muscle as a secretory organ is now well known but we can only speculate as to the existence of as-yet-unidentified myokines, especially those upregulated in response to muscle contractile activity. We first attempted to establish an “insert-chamber based in vitro exercise model” allowing the miniature but high cell-density culture state enabling highly developed contractile human myotubes to be readily obtained by applying electric pulse stimulation (EPS). By employing this in vitro exercise model, we identified R-spondin 3 (RSPO3) as a novel contraction-inducible myokine produced by cultured human myotubes. Contraction-dependent muscular RSPO3 mRNA upregulation was confirmed in skeletal muscles of mice subjected to sciatic nerve mediated in situ contraction as well as those of mice after 2 h of running. Pharmacological in vitro experiments demonstrated a relatively high concentration of metformin (millimolar range) to suppress the contraction-inducible mRNA upregulation of human myokines including RSPO3, interleukin (IL)-6, IL-8 and CXCL1. Our data also suggest human RSPO3 to be a paracrine factor that may positively participate in the myogenesis processes of myoblasts and satellite cells. Thus, the “insert chamber-based in vitro exercise model” is a potentially valuable research tool for investigating contraction-inducible biological responses of human myotubes usually exhibiting poorer contractility development even in the setting of EPS treatment.
Survival comparison between postoperative and preoperative radiotherapy for stage I–III non-inflammatory breast cancer
Authors: Zhang Yuxi , Xu Zhipeng , Chen Hui , Sun Xinchen , Zhang Zhaoyue
Published: August 2022

To compare the survival benefit between preoperative and postoperative radiotherapy for stage I–III non-inflammatory breast cancer patients, we conducted a retrospective cohort study using surveillance, epidemiology and end results databases. Our study recruited patients who had been diagnosed with stage I–III breast cancer and underwent surgery and radiotherapy. The overall survival was calculated by Kaplan–Meier method. Cox risk model was used to determine the impact of radiotherapy according to stage, molecular subtype and other risk factors. Propensity score matching was used to balance measurable confounding factors. Of all the 411,279 enrolled patients varying from 1975 to 2016, 1712 patients received preoperative radiotherapy, and 409,567 patients received postoperative radiotherapy. Compared with the postoperative radiotherapy group, the preoperative radiotherapy group showed significantly higher risks of overall mortality and breast cancer-specific mortality. Survival differences in treatment sequences were correlated with stage, molecular subtypes and other risk factors. According to the results of this study, preoperative radiotherapy did not show a survival advantage, and postoperative radiotherapy is still the primary treatment. However, preoperative radiotherapy also has some theoretical advantages, such as phase reduction and recurrence reduction. Therefore, it is still worthy of further exploration.
Cardiac safety analysis of anti-HER2-targeted therapy in early breast cancer
Authors: Zhang Li , Wang Yan , Meng Wenjing , Zhao Weipeng , Tong Zhongsheng
Published: August 2022

To evaluate the cardiac safety of anti-HER2-targeted therapy for early breast cancer; to investigate whether trastuzumab combined with pertuzumab increases cardiac toxicity compared with trastuzumab; to evaluate the predictive value of high-sensitivity Troponin (hs-TnI) and QTc for the cardiotoxicity associated with anti-HER2 targeted therapy in early breast cancer. A total of 420 patients with early-stage HER2-positive breast cancer who received trastuzumab or trastuzumab combined with pertuzumab for more than half a year in Tianjin Medical University Cancer Hospital from January 2018 to February 2021 were included. Left ventricle ejection fraction (LVEF), hs-TnI values, and QTc were measured at baseline and 3, 6, 9, 12 months. Cardiotoxicity was defined as a decrease in LVEF of at least 10 percentage points from baseline on follow-up echocardiography. Cardiotoxicity developed in 67 of the 420 patients (15.9%) and all patients had LVEF above 50% before and after treatment. The incidence of cardiotoxicity in trastuzumab and trastuzumab combined with pertuzumab was 14.3% and 17.9%, respectively (P > 0.05). Logistic regression analysis showed that age, coronary heart disease, left chest wall radiotherapy, and anthracyclines sequential therapy were independent risk factors for cardiotoxicity (P < 0.05). The value of hs-TnI and QTc at the end of treatment (12th month) were selected for ROC curve prediction analysis and the area under the ROC curve was 0.724 and 0.713, respectively, which was significantly different from the area of 0.5 (P < 0.05). The decrease of LVEF in the study was mostly asymptomatic, from the heart safety point of view, the anti-HER2 targeted therapy for early breast cancer was well tolerated. Trastuzumab combined with pertuzumab did not significantly increase cardiotoxicity. However, subgroup analysis suggests that in the presence of coronary artery disease (CAD) and sequential treatment with anthracene, trastuzumab and pertuzumab may increase the cardiac burden compared with trastuzumab. Hs-TnI and QTc may be useful in monitoring and predicting cardiotoxicity associated with anti-HER2 targeted therapy for early breast cancer.
Consumption of soft drinks rich in phosphoric acid versus struvite crystallization from artificial urine
Authors: Skubisz Mikołaj , Torzewska Agnieszka , Mielniczek-Brzóska Ewa , Prywer Jolanta
Published: August 2022

In recent years, there has been a continuous increase in the incidence of urolithiasis, especially in highly developed countries. Therefore, the question arises which factors specific to these countries may be responsible for the increase in the incidence of this disease. In this article, we try to assess the effect of phosphoric acid, a component of various carbonated drinks, including Coca-Cola, on the nucleation and growth of struvite crystals, which are the main component of infectious urinary stones. The research was carried out in the environment of artificial urine with and without the presence of Proteus mirabilis bacteria. In the latter case, the activity of bacterial urease was simulated by adding an aqueous ammonia solution. The obtained results indicate that phosphoric acid present in artificial urine causes the nucleation of struvite to shift towards a lower pH, which means that struvite nucleates earlier in artificial urine compared to the control test. The amount of struvite formed is the greater the higher the concentration of phosphoric acid. At the same time, as the concentration of phosphoric acid increases, the growing struvite crystals are larger, which is disadvantageous because they are more difficult to remove from the urinary tract along with the urine. For the highest levels of phosphoric acid tested, large dendrites are formed, which are particularly undesirable as they can damage the epithelium of the urinary tract. The effect of phosphoric acid on the nucleation and growth of struvite is explained in base of chemical speciation analysis. This analysis indicates that the MgHCit and MgCit− complexes have the main influence on the nucleation and growth of struvite in artificial urine in the presence of phosphoric acid. It should be keep in mind that all these effects of phosphoric acid are possible when the urinary tract is infected with urease-positive bacteria. In the absence of infection, phosphoric acid will not cause struvite to crystallize.
Development and evaluation of the HRSD-D, an image-based digital measure of the Hamilton rating scale for depression
Authors: Berko Adi , Bar-Sella Avigail , Fisher Hadar , Sobolev Michael , Pollak J. P. , Zilcha-Mano Sigal
Published: August 2022

The Hamilton rating scale for depression (HRSD) is considered the gold standard for the assessment of major depressive disorder. Nevertheless, it has drawbacks such as reliance on retrospective reports and a relatively long administration time. Using a combination of an experience sampling method with mobile health technology, the present study aimed at developing and conducting initial validation of HRSD-D, the first digital image-based assessment of the HRSD. Fifty-three well-trained HRSD interviewers selected the most representative image for each item from an initial sample of images. Based on their responses, we developed the prototype of HRSD-D in two versions: trait-like (HRSD-DT) and state-like (HRSD-DS). HRSD-DT collects one-time reports on general tendencies to experience depressive symptoms; HRSD-DS collects daily reports on the experience of symptoms. Using a total of 1933 responses collected in a preclinical sample (N = 86), we evaluated the validity and feasibility of HRSD-D, based on participant reports of HRSD-DT at baseline, and 28 consecutive daily reports of HRSD-DS, using smartphone devices. HRSD-D showed good convergent validity with respect to the original HRSD, as evident in high correlations between HRSD-DS and HRSD (up to Bstd = 0.80). Our combined qualitative and quantitative analyses indicate that HRSD-D captured both dynamic and stable features of symptomatology, in a user-friendly monitoring process. HRSD-D is a promising tool for the assessment of trait and state depression and contributes to the use of mobile technologies in mental health research and practice.
Fine-scale movement of northern Gulf of Mexico red snapper and gray triggerfish estimated with three-dimensional acoustic telemetry
Authors: Bohaboy Erin C. , Cass-Calay Shannon L. , Patterson William F.
Published: August 2022

Red snapper and gray triggerfish are ecologically, economically, and culturally important reef fishes in the northern Gulf of Mexico (nGOM). Scientists and managers have sought to understand the effects of artificial reefs on reef fish ecology by focusing on fish residency and movement at artificial reefs with less attention paid to broader spatial and temporal patterns in reef fish movements among a seascape of artificial reefs and other natural habitats. We used novel large-scale (> 15 km2) geopositioning acoustic telemetry arrays to track the 3-dimensional movements of tagged red snapper (n = 59) and gray triggerfish (n = 15) among multiple nGOM artificial reefs up to 333 days. Tagged fish moved frequently among artificial reefs and had shorter residence times at the release reef (43 days for red snapper and 3 days for gray triggerfish) than reported in previous studies. Both species displayed high individual variability in movement dynamics, as well as seasonally variable diel patterns of habitat use, height above bottom, and distance to reefs, which may have been driven by dynamic influences of predation risk, physiological constraints, or foraging over time and space. The wider seascape view revealed in this study demonstrates the importance of including multiple artificial reefs over long timescales to capture individual, spatial, and temporal variability in reef fish movement.
Salinity indicators in sediment through the fluvial-to-marine transition (Fraser River, Canada)
Authors: Dashtgard Shahin E. , Wang Aihua , Pospelova Vera , Wang Pei-Ling , La Croix Andrew , Ayranci Korhan
Published: August 2022

Many sediment attributes have been proposed as proxies for determining salinity conditions under which sediment is deposited, and six attributes (Sr/Ba-HAc, Sr/Ba-NH4Ac, δ13Corg, C/N, and the relative abundances and concentrations of dinoflagellate cysts) are compared here. In this paper, sediment attributes from the Fraser River Delta, Canada and surrounding coastal areas are compared by depositional position along the fluvial-to-marine transition, by salinity, and by sedimentological characteristics. Along the fluvial-to-marine transition, most attributes exhibit distinct trends between parts of the river that experiencesustainedmarine water (saltwater) influence over seasonal and tidal timeframes, and parts that experience only freshwater or periodic saltwater influence. No attributes are reliable indicators of depositional position where saltwater incursion is short lived or where water is fresh. Where marine influence is sustained, Sr/Ba-HAc and Sr/Ba-NH4Ac are the most reliable positional indicators along the fluvial-to-marine transition. When compared strictly to salinity, Sr/Ba-HAc, Sr/Ba-NH4Ac, and δ13Corgall correlate predictably except in delta front and prodelta settings. Our data show that all six sediment attributes are heavily impacted by river-derived sedimentation, and it is not appropriate to compare values from strongly river-influenced settings (e.g., deltas) with those from weakly river-influenced settings (e.g., bays and estuaries).
A compact and wideband MIMO antenna for high-data-rate biomedical ingestible capsules
Authors: Alazemi Abdullah J. , Iqbal Amjad
Published: August 2022

Due to recent advancements in complementary metal-oxide-semiconductor (CMOS) cameras, transferring high resolution images and videos are possible in wireless capsule endoscopy. High-data-rates communication is required for such data, which is possible using multiple-input-multiple-output (MIMO) antennas. In this paper, a low-sized, compact, high-data-rate, highly isolated two-element MIMO antenna with a large bandwidth has been proposed at 2.45 GHz for wireless capsule endoscopy. The geometry of the antenna ($$5\times 4.2 \times 0.12\,\hbox {mm}^{3}$$

5
×
4.2
×
0.12

mm
3

) is kept small using meandered geometry, defected ground structure, and high permittivity of the substrate. A wider bandwidth of 620 MHz (2.15–2.77 GHz) is achieved by exciting dual-modes of the antenna using defected ground structure. Furthermore, a lower mutual coupling between the antennas (30.1 dB at 2.45 GHz) is realized, despite the small edge-to-edge gap of 0.5 mm, using combination of defected ground structure and I-shaped stub. Keeping in mind of system level configuration, this antenna is simulated and measured inside a capsule device by considering effects of the other components and the device itself. The practical measurements are performed by inserting the capsule device (containing the MIMO antenna) inside minced meat. To check the safety and effectiveness of the proposed MIMO antenna, it’s specific absorption rate (SAR) and link budget are calculated and validated. In addition, the $$2\times 2$$

2
×
2

channel specifications are verified which shows satisfactory performance. This antenna has high channel capacity ($$\approx 8.2 \,\hbox {bps/Hz}$$

≈
8.2

bps/Hz

at $$\hbox {SNR} = 20 \,\hbox {dB}$$

SNR
=
20

dB

) than single-input-single-output (SISO) antennas, thus, is a suitable choice for high-data-rate capsule endoscopic devices. To the best of the authors’ knowledge, this is the first implantable MIMO antenna reported so far with such lower dimension and wider bandwidth.
The impact of H/D exchange on the thermal and structural properties as well as high-pressure relaxation dynamics of melatonin
Authors: Jesionek Paulina , Hachuła Barbara , Heczko Dawid , Jurkiewicz Karolina , Tarnacka Magdalena , Zubko Maciej , Paluch Marian , Kamiński Kamil , Kamińska Ewa
Published: August 2022

In this paper, thermal properties, atomic-scale structure, and molecular dynamics (at ambient and high pressure) of native melatonin (MLT) and its partially-deuterated derivative (MLT-d2) have been investigated. Based on infrared spectroscopy, it was shown that treating MLT with D2O causes the replacement of hydrogen atoms attached to the nitrogen by deuterium. The degree of such substitution was very high (> 99%) and the deuterated sample remained stable after exposure to the air as well as during the melting and vitrification processes. Further calorimetric studies revealed the appearance of a peculiar thermal event before the melting of crystalline MLT-d2, which was assigned by the X-ray diffraction to a local negative thermal expansion of the unit cell. Finally, the high-pressure dielectric experiments indicated a few interesting findings, including the variation in the shape of the structural relaxation peak during compression, the difference in the pressure evolution of the glass transition temperature, and the temperature dependence of activation volume for both MLT species. The variations in these parameters manifest a different impact of the compression/densification on the dynamics of hydrogen and deuterium bonds in the native and partially-deuterated MLT, respectively.
Estimating the time of last drinking from blood ethyl glucuronide and ethyl sulphate concentrations
Authors: Wang Lele , Zhang Wei , Wang Ruilong , Guang Yongli , Zhang Daming , Zhang Chao , Hu Meng , Wei Zhiwen , Zhang Wenfang , Yun Keming , Guo Zhongyuan
Published: August 2022

The determination of length of time from the last drinking is critical for cases like drunk driving, sexual assault victims, and also postmortem suspected poisoning cases. The study was aimed to established a method of estimating the time of last drinking through the pharmacokinetic study of conjugation metabolites of alcohol in blood after a single oral dose. Twenty-six volunteers (14 males) consumed alcohol with food at a fixed dose of 0.72 g/kg after fasting for 12 h. Five milliliters of blood were collected 120 h after the start of drinking, and all samples were analyzed with headspace-gas chromatography and high-performance liquid chromatography–tandem mass spectrometry. The time point of last drinking was estimated through the relationship between the concentration ratio of ethyl glucuronide to ethyl sulphate and the length of time after drinking. Pharmacokinetic parameters were analyzed by a pharmacokinetic software DAS according to the non-compartment model. A good correlation model was obtained from the relationship between concentration ratio of ethyl glucuronide to ethyl sulphate in blood and the time of alcohol use, and the margin of error was mostly lower than 10%. The time of maximum concentration, maximum concentration, and elimination half-life of ethyl glucuronide in blood were 4.12 ± 1.07 h, 0.31 ± 0.11 mg/L and 2.56 ± 0.89 h; the time of maximum concentration, maximum concentration, and elimination half-life of ethyl sulphate in blood were 3.02 ± 0.70 h, 0.17 ± 0.04 mg/L, and 2.04 ± 0.76 h. The study established a potential method to estimate the length of time after a moderate oral dose, and provided pharmacokinetic parameters of ethyl glucuronide and ethyl sulphate in Chinese population.
Alpinumisoflavone ameliorates choroidal neovascularisation and fibrosis in age-related macular degeneration in in vitro and in vivo models
Authors: Yu Eunhye , Song Yunjeong , Gu Sun Mi , Jo Yang Hee , Yeon Sang Won , Han Kyu Jin , Lee Mi Kyeong , Min Jung Kee , Yun Jaesuk
Published: August 2022

Age-related macular degeneration (AMD) is a major cause of vision loss in the elderly population. Anti-vascular endothelial growth factor (VEGF) antibody therapy is applicable to neovascularisation of AMD; however, the prevention of fibrosis after anti-VEGF monotherapy is an unmet medical need. Subretinal fibrosis causes vision loss in neovascular age-related macular degeneration (nAMD) even with anti-VEGF therapy. We report the anti-fibrotic and anti-neovascularisation effects of alpinumisoflavone (AIF), an isoflavonoid derived from unripe Maclura tricuspidata fruit, in in vitro and in vivo models. For in vitro study, we treated H2O2 or THP-1 conditioned media (TCM) following activation with phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) in a human retinal pigment epithelial cell line (ARPE-19). Choroidal neovascularisation (CNV) was induced by laser photocoagulation in mice, immediately followed by intravitreal administration of 25 μg AIF. CNV area and fibrosis were measured 7 days after laser photocoagulation. AIF showed anti-fibrosis and anti-neovascularisation effects in both the models. The laser induced CNV area was reduced upon AIF administration in nAMD mouse model. Additionally, AIF decreased the levels of the cleaved form of crystallin alpha B (CRYAB), a chaperone associated with VEGF stabilisation and fibrosis. Our results demonstrate a novel therapeutic application of AIF against neovascularisation and fibrosis in nAMD.
Non-stationary time-varying vehicular channel characteristics for different roadside scattering environments
Authors: Li Changzhen , Chen Wei , Pei Zhonghui , Chang Fuxing , Yu Junyi , Luo Fan
Published: August 2022

With the deep integration of wireless communication technology and automobile industry, vehicular communication has become one of the key technologies supporting the development of Internet-of-vehicle. Due to the high-speed mobility of vehicles and the rapid change of the propagation environments, vehicle-to-vehicle (V2V) wireless communication channels are generally non-stationary. Meanwhile, the variability of V2V channel characteristics is obvious in different scattering environments. Focusing on these research points, this paper presents the analysis and comparison of V2V channel characteristics for different scattering scenarios based on a series of 5.9 GHz channel measurements. The measurement data are collected from the iron bridge, the soundproof wall, and the road lamp scenarios. The stationary time and frequency are investigated on the basis of method of local scattering functions. The classical channel characteristics, including power delay profile, Ricean K-factor, root means square (RMS) delay spread and RMS Doppler spread are extracted following the propagation principle. Furthermore, considering the source and birth-death process of multi-path components (MPCs) in different scattering propagation environments, cluster identification and statistical results are presented and compared. The different values of the channel parameters and the different performance of the channel under different scattering environments can help us understand the V2V channel deeply. The research results can be used for the design and optimization of vehicular communication systems in different scattering environments.
The effect of influenza vaccine in reducing the severity of clinical outcomes in patients with COVID-19: a systematic review and meta-analysis
Authors: Almadhoon Hossam Waleed , Hamdallah Aboalmagd , Elsayed Sarah Makram , Hagrass Abdulrahman Ibrahim , Hasan Mohammed Tarek , Fayoud Aya Mamdouh , Al-kafarna Mohammed , Elbahnasawy Mohammad , Alqatati Fadel , Ragab Khaled Mohamed , Zaazouee Mohamed Sayed , Hasabo Elfatih A.
Published: August 2022

Recent evidence suggests that vaccination against influenza may reduce the clinical outcomes of COVID-19. This study looked at the link between influenza vaccination and the severity of COVID-19 infection. We searched five databases until August 2021. We included studies that reported the relationship between influenza vaccination and COVID-19 outcomes. We pooled the data as risk ratio (RR) or mean difference (MD), with 95% confidence intervals (CIs), the data pooled using fixed and random effects models according to the heterogeneity of results. Sixteen observational studies with 191,496 COVID-19 patients were included. In terms of mechanical ventilation, our analysis showed a significant favor for the influenza vaccinated group over the non-vaccinated group (RR = 0.72, 95% CI [0.54, 0.96], P = 0.03). However, the analysis indicated no statistically significant differences between vaccinated and non-vaccinated groups in the term of mortality rate (RR = 1.20, 95% CI [0.71, 2.04], P = 0.50), hospital admissions (RR = 1.04, 95% CI [0.84, 1.29], P = 0.75), intensive care admissions (RR = 0.84, 95% CI [0.44, 1.62], P = 0.60). There were no significant differences between those who had received the influenza vaccine and those who had not in COVID-19 clinical outcomes, except for mechanical ventilation which showed a significantly lower risk in the influenza vaccinated group compared to the non-vaccinated one. However, future research is encouraged as our data have limitations, and the influenza vaccine is regularly updated. Also, this does not exclude the importance of the influenza vaccine during the COVID-19 pandemic.
Birth related parameters are important contributors in autism spectrum disorders
Authors: Banerjee Nilanjana , Adak Pallabi
Published: August 2022

Autism spectrum disorders is a group of childhood onset neurodevelopmental disorders affecting millions of children across the globe. Characterised by age inappropriate lack of reciprocal social interaction, repetitive behaviours and deficits in communication skills, it has been found to have genetic, epigenetic and environmental contributions. In this work, we wanted to identify the effects of birth related parameters on the disease pathogenesis in an exposed population of West Bengal, India. We have considered age of both parents at birth, difference in parental age, familial history of mental illness, delay in developmental-milestones, birth-weight, birth-order, birth-term, mode of delivery and gestational complications as contributors. We found the parental age and their age difference to be the most important contributors towards ASD in this population. Birth order, sex of the probands, complications during gestation, birth weight, family history of mental illness and birth history also contributed to the condition, although to a lesser extent. Since such types of data are lacking in Indian population, this report adds useful information to the relevant field.
Relationship between lipid accumulation product and oxidative biomarkers by gender in adults from Yucatan, Mexico
Authors: Lugo Roberto , Avila-Nava Azalia , Pech-Aguilar Alfredo Geovanny , Medina-Vera Isabel , Guevara-Cruz Martha , Gutiérrez Solis Ana Ligia
Published: August 2022

Excessive adipose tissue can lead to metabolic abnormalities resulting in lipid alteration and oxidative stress (OS) status. The lipid accumulation product (LAP) index is a biomarker that indicates central lipid accumulation and has been proposed as an accurate and independent indicator of risk for several cardiometabolic related conditions. There is a lack of information about the possible association of LAP and OS biomarkers. Therefore, this work aimed to investigate the relationship between LAP and OS biomarkers in adults. A cross-sectional study was performed in 250 subjects attending the Hospital Regional de Alta Especialidad de la Península de Yucatán. Anthropometrical and clinical parameters were measured. The serum oxidative biomarkers such as malondialdehyde (MDA) and total antioxidant capacity (TAC) were evaluated by spectrophotometry and by the oxygen radical absorbance capacity (ORAC), respectively. A positive and significant correlation between serum levels of MDA and LAP (r = 0.162, p = 0.010) was observed. This relationship was stronger in women (r = 0.189, p = 0.013) than in men. The association between them remained significant after adjusting for confounders (r = 0.23, p < 0.001). A cutoff of LAP of 73.73 predicts high levels of MDA in women aged between 40 and 59. LAP index was associated with OS biomarkers in women and men from Yucatan, Mexico. Therefore, the elevation of the LAP index could identify an imbalance in the redox status.
Study on image data cleaning method of early esophageal cancer based on VGG_NIN neural network
Authors: Li Zhengwen , Wu Runmin , Gan Tao
Published: August 2022

In order to clean the mislabeled images in the esophageal endoscopy image data set, we designed a new neural network VGG_NIN. Based on the new neural network structure, we developed a method to clean the mislabeled images in the esophageal endoscopy image data set. To verify the effectiveness of the proposed method, we designed two experiments using 3835 esophageal endoscopy images provided by West China Hospital of Sichuan University. The experimental results showed that the proposed method could clean about 93% of the mislabeled images in the data set, which was the first time in the cleaning of esophageal endoscopy image data set. Finally, in order to verify the generalization ability of this method, we cleaned the Kaggle open cat and dog data set, and cleaned out about 167 mislabeled images. Therefore, the proposed method can effectively screen the mislabeled images in the esophageal endoscopy image data set and has good generalization ability, which can provide great help for the development of high-performance gastrointestinal endoscopy image analysis model.
Dental biorhythm is associated with adolescent weight gain
Authors: Mahoney Patrick , McFarlane Gina , Loch Carolina , White Sophie , Floyd Bruce , Dunn Erin C. , Pitfield Rosie , Nava Alessia , Guatelli-Steinberg Debbie
Published: August 2022

Background

Evidence of a long-period biological rhythm present in mammalian hard tissue relates to species average body mass. Studies have just begun to investigate the role of this biorhythm in human physiology.

Methods

The biorhythm is calculated from naturally exfoliated primary molars for 61 adolescents. We determine if the timing relates to longitudinal measures of their weight, height, lower leg length and body mass collected over 14 months between September 2019 to October 2020. We use univariate and multivariate statistical analyses to isolate and identify relationships with the biorhythm.

Results

Participants with a faster biorhythm typically weigh less each month and gain significantly less weight and mass over 14-months, relative to those with a slower biorhythm. The biorhythm relates to sex differences in weight gain.

Conclusions

We identify a previously unknown factor that associates with the rapid change in body size that accompanies human adolescence. Our findings provide a basis from which to explore novel relationships between the biorhythm and weight-related health risks.
Introduction
Authors: Bui Mai , Mac Dowell Niall
Published: August 2022

Greenhouse gas removal (GGR) technologies, also known as carbon dioxide removal (CDR), are understood to be essential to all scenarios consistent with a 1.5 °C target. Technologies that provide net removal of CO2 emissions include nature-based solutions (e.g., afforestation, reforestation and coastal blue carbon), enhanced weathering, biochar, soil carbon sequestration, ocean fertilisation, direct air capture of CO2 (DAC) and bioenergy with carbon capture and storage (BECCS). Advancing these GGR technologies forward will require scale-up and commercialisation. Importantly, we will need to identify which GGR pathways can be relied upon to be a material option within the portfolio of solutions, and which will be niche players. The key areas of GGR that require further development in understanding include the climate repair value, the permanence of the carbon removal (e.g., one year or 1000 years), the types of services provided (e.g., environmental, energy) by each GGR option, how cost might vary in different regions, and the protocols for monitoring, reporting and verification (MRV).
Greenhouse Gas Removal: Overview and Current Status of Deployment
Authors: Pisciotta Maxwell , Davids Justine , Wilcox Jennifer
Published: August 2022

Greenhouse gas removal (GGR) plays an increasingly important role in meeting the climate goals of keeping the global temperature rise below 2 °C above that of the preindustrial era. There are many GGR methods which can achieve negative emissions, each with their own advantages and drawbacks. This overview chapter provides insight into the challenges of removing CO2 from the air while highlighting opportunities for the GGR technologies discussed throughout the rest of the book, and considerations facing their future development and deployment.
Accounting Negative Emissions. How Difficult Could It Be?
Authors: Ramírez Andrea Ramírez
Published: August 2022

Negative emissions will play a key role in our ability to reach ambitious climate targets. Although carbon accounting appears a simple task, the lack of agreed definition, accounting methodologies and assumptions will hinder decision makers from appropriately and efficiently incentivising, deploying, monitoring and verifying those emissions. This chapter highlights the importance of appropriately defining and accounting emissions by exploring four key challenges for carbon accounting of negative emissions, namely: assessing permanence, accounting for the temporal distribution of emissions and removals, choosing time horizons, and conflating CO2 avoidance and CO2 negativity.