bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Caragana microphylla Lam. on Desertified Grassland Restoration
Authors: Zhu Tiantian , Li Qinghe
Published: October 2024

Background: The restoration of the degraded sandy grasslands in Hulun Buir is crucial for maintaining the local ecological balance and sustainable development. Caragana microphylla Lam., a shrub species widely employed in the restoration of sandy vegetation. It is essential to understand its impact on the understory vegetation and soil properties during this process. Methods: This study employed ANOVA, Pearson correlation, and redundancy analysis to systematically analyze the impact of C. microphylla on the three critical stages of desertified grassland vegetation recovery: semi-fixed dunes, fixed dunes, and sandy grasslands. It provided strategies for the restoration of desertified grassland vegetation and offered additional theoretical evidence for the role of vegetation in promoting the recovery of sandy lands. Results: (1) As the degree of vegetation recovery in desertified grasslands increases, the species richness of understory vegetation, Shannon–Wiener index, community height, and biomass also increase. Both the community height and biomass within shrublands are higher than outside, with species richness within the shrublands being higher than outside during the semi-fixed and fixed-sand land stages. (2) In both the 0~10 cm and 10~20 cm soil layers, soil water content showed an increasing trend, peaking in the sandy grassland stage (1.2%), and was higher within the shrublands than outside. The soil water content at 10~20 cm was higher than in the 0~10 cm layer. In both layers, clay and silt content gradually increased with the degree of vegetation recovery in the sandy land, and higher within the shrublands than outside, while the opposite was true for sand content. (3) In both soil layers, soil organic carbon gradually increased with the degree of vegetation recovery, peaking in the sandy grassland stage (4.12 g·kg−1), and was higher within the shrublands than outside. Total nitrogen increased from the semi-fixed-sand land stage to the fixed-sand land stage, with higher levels within the shrublands than outside at all stages. Soil pH within the shrublands decreased as the degree of vegetation recovery increased. There was no significant change in the total phosphorus content. (4) In both soil layers, soil physicochemical characteristics accounted for 59.6% and 46.9% of the vegetation changes within and outside the shrublands, respectively, with the main influencing factors being the soil particle size, total nitrogen, soil water content, and soil organic carbon. Conclusions: In the process of sandy grassland restoration, C. microphylla facilitates the growth and development of vegetation by enhancing the underlying soil physicochemical properties, specifically regarding the soil particle size distribution, soil water content, soil organic carbon, and total nitrogen.
The Effect of Adding Green and Black Tea Waste Extracts on Rumen Fermentation Parameters by In Vitro Techniques
Authors: Paya Hamid , Gheshlagh Nazak Shokrani , Taghizadeh Akbar , Besharati Maghsoud , Lackner Maximilian
Published: October 2024

The increase in global temperatures over the past few decades due to greenhouse gas emissions has raised concerns and necessitated further research in climate change mitigation and adaptation. Methane is a prominent greenhouse gas that significantly contributes to climate change, with a substantial amount generated through fermentation processes occurring in the rumen of ruminant animals. The potential of plant secondary metabolites, especially those derived from tannin-rich plants, warrants investigation to modify rumen fermentation and mitigate methane emissions in livestock diets. The objective of this study was to assess the impact of extracts obtained from green and black tea waste on rumen fermentation dynamics and gas (methane) production, utilizing in vitro methods. For this purpose, rumen fluid was collected from two fistulated sheep and subjected to three treatments: (1) a basal diet (control), (2) a basal diet + green tea waste extract (5% of dry matter), (3) a basal diet + black tea waste extract (5% of dry matter). The study assessed the effects of incorporating extracts from green and black tea waste on various parameters, including digestibility, protozoa population, ammonia nitrogen levels, volatile fatty acids, and methane gas production following a 24-h incubation period. Statistical analysis of the data was conducted using SAS software within a completely randomized design framework. The findings indicated that the addition of green and black tea waste extracts significantly decreased methane gas production (p < 0.05), protozoa count (p < 0.05), and ammonia nitrogen concentrations in rumen fluid (p < 0.05) when compared to the control group. The addition of green and black tea waste extracts has significantly altered the concentration of VFAs in rumen fluid (p < 0.05). Specifically, the addition of green tea waste extract has led to a highly significant reduction in acetic acid, (p < 0.01) and the addition of both extracts has resulted in a significant increase in propionic acid (p < 0.05). Consequently, the results suggest that the inclusion of green and black tea waste extracts in livestock diets may effectively mitigate methane emissions in the rumen, thereby reducing feed costs and reducing environmental pollution.
Enzymes in Biorefinery
Authors: Sohail Muhammad
Published: October 2024

Biorefineries integrate various processes to obtain different products from a single waste material [...]
Bacillus clausii: A Review into Story of Its Probiotic Success and Potential Food Applications
Authors: Sadrimovahed Mahtab , Ulusoy Beyza H.
Published: October 2024

Probiotics are live microbiota that can confer their hereditary health benefits upon the host. They can positively alter the diversity of the host’s gut microbiota population. Bacillus clausii is a spore-producing potential probiotic. Its application in the food industry has been highly recommended. Clausii spores are greatly resistant to harsh food processing treatment (heat and industrial pressure) and they can maintain their physiological traits (acid, bile salt) inside the human gut. The utilization of spores can enhance the nutritional viability of livestock, as well as the functionality of on-shelf products. This contemporary review covers the great attributes of B. clausii, including physiological characteristics, modes of action, probiotics benefits, a safety assessment, and the bacteria food industry applications for the purposes of producing enhanced functional foods known as probiotic foods. To our knowledge, although B. clausii has been widely applied in food industry, the amount of the literature that is dedicated to its role in sustainable food production and addresses its potential sporogenous probiotic behavior is fewer as compared to non-sporogenous lactic acid bacteria (Lactobacillus spp. and Bifidobacterium spp.). In our review, we also discovered a lack of empirical evidence on the synbiotic and synergistic behavior of clausii in combination with other active nutrients. Therefore, our review suggests that more studies should be conducted on the potential probiotic contribution of B. clausii in sustainable food production and its synergistic and synbiotic behavior in conjunction with other active nutrients.
Malware Detection Based on API Call Sequence Analysis: A Gated Recurrent Unit–Generative Adversarial Network Model Approach
Authors: Owoh Nsikak , Adejoh John , Hosseinzadeh Salaheddin , Ashawa Moses , Osamor Jude , Qureshi Ayyaz
Published: October 2024

Malware remains a major threat to computer systems, with a vast number of new samples being identified and documented regularly. Windows systems are particularly vulnerable to malicious programs like viruses, worms, and trojans. Dynamic analysis, which involves observing malware behavior during execution in a controlled environment, has emerged as a powerful technique for detection. This approach often focuses on analyzing Application Programming Interface (API) calls, which represent the interactions between the malware and the operating system. Recent advances in deep learning have shown promise in improving malware detection accuracy using API call sequence data. However, the potential of Generative Adversarial Networks (GANs) for this purpose remains largely unexplored. This paper proposes a novel hybrid deep learning model combining Gated Recurrent Units (GRUs) and GANs to enhance malware detection based on API call sequences from Windows portable executable files. We evaluate our GRU–GAN model against other approaches like Bidirectional Long Short-Term Memory (BiLSTM) and Bidirectional Gated Recurrent Unit (BiGRU) on multiple datasets. Results demonstrated the superior performance of our hybrid model, achieving 98.9% accuracy on the most challenging dataset. It outperformed existing models in resource utilization, with faster training and testing times and low memory usage.
The Personality Traits and Interactive Behavior of Chinese Mitten Crab (Eriocheir sinensis)
Authors: Sun Jianyang , Zhang Dongxin , Hong Yuhang , Weng Chengyi , Pang Yangyang , Cheng Yongxu , Yang Xiaozhen
Published: October 2024

Agonistic behavior is a common behavior among agonistic Chinese mitten crabs (Eriocheir sinensis). Such behavior often leads to limb loss or physical impairment, and significantly affects the survival, growth, and quality of the crabs, and even the yield and economic value for E. sinensis. Agonistic behavior often occurs in agonistic crabs, which is closely related to personality traits and interactive behavior of animals. E. sinensis has personality traits such as boldness, aggression, and exploration as evidenced by the partition-crossing experiment, mirror experiment, and shelter experiment. Agonistic crabs were identified as individuals with boldness, high aggression, and high exploration. The interactive behavior spectrum of E. sinensis was first obtained, consisting of 15 behaviors. This spectrum described and summarized all interactive behaviors of E. sinensis during fights. The interactive behavior characteristics of agonistic crabs were identified as darting, intimidating, grabbing, pushing, stretching, and visitation. These results lay a theoretical basis for in-depth behavioral research on E. sinensis in the future. The technique of identifying agonistic crabs by personality traits and interactive behaviors not only allows for the elimination of agonistic crabs from the aquaculture process and the reduction in negative impacts caused by aggressive crabs, but also allows for the breeding of non-agonistic crabs and the further reduction in the economic losses caused by fighting behaviors.
Observation and Analysis of In Vitro Digestibility of Different Breads Using a Human Gastric Digestion Simulator
Authors: Shibasaki Motomi , Maeda Tatsuro , Tanaka Takayoshi , Sugiyama Kenjiro , Kozu Hiroyuki , Noguchi Ritsuna , Umeda Takumi , Araki Tetsuya , Kobayashi Isao
Published: October 2024

The digestion behavior of a food bolus comprising bread particles in the presence of gastric peristalsis remains poorly understood. This study systematically investigated the effect of bread type on in vitro gastric digestion behavior using a human gastric digestion simulator (GDS) that is capable of quantitatively simulating gastric peristalsis. A food bolus consisting of 60 g of bread (white bread, bagel, German bread, French bread, or croissant), 15 mL of a simulated salivary fluid, and 240 mL of a simulated gastric fluid was used for gastric digestion in vitro using the GDS for 3 h at 37 °C. Direct observation of the gastric digestion behavior in the GDS vessel demonstrated that the structure and composition of breads considerably influenced the physical digestion processes of bread particles. These processes include their fracture, rubbing, disintegration, swelling owing to the penetration of gastric fluid, and release of fat from their surface. Fluorescence microscopy enabled an improved understanding of the variations in the microstructure and major component distribution of the breads during the gastric digestion in vitro. The results showed how the different breads influenced gastric digestibility in vitro through quantitative gastric peristalsis. The GDS can also be applicable to studying gastric digestibility in vitro of other types of bread.
Dissipation and Residue of Chloroisobromine Cyanuric Acid in Ginger and Pepper and Its Dietary Intake Risk Assessment
Authors: Hao Yadong , Li Yingxuan , Wang Jue , Pang Sen , Li Shuang
Published: October 2024

Chloroisobromine cyanuric acid is a highly effective broad-spectrum systemic fungicide for disease control in agricultural crops. In this study, the degradation, residue levels, and potential dietary risks associated with the chloroisobromine cyanuric acid residues in two widely consumed crops, pepper and ginger, were evaluated through supervised field experiments conducted at 12 sites for pepper and 4 sites for ginger in China in 2021. A QuEChERS-LC-MS/MS method was established for quantifying cyanuric acid (CYA) in both crops. The method achieved a limit of quantification (LOQ) of 0.02 mg kg−1 for ginger and 0.05 mg kg−1 for pepper, with recovery rates ranging from 91% to 96% for ginger and 84% to 89% for pepper and the relative standard deviation of 2.9% to 11.2% for ginger and 2.8%~12.9% for pepper, respectively. The results indicated that CYA had half-lives ranging from 3.1 to 8.2 days in pepper, and the terminal CYA residues at harvest were all lower than 5 mg kg−1, the maximum residue limit established in China. Furthermore, the chronic dietary risk exposure to chloroisobromine cyanuric acid in ginger and pepper, harvested at a pre-harvest interval of three days and at the normal harvesting time after the application of 50% chloroisobromine cyanuric acid soluble powder (SP), was 37.96%, which was much lower than 100%, indicating no significant health risks to the Chinese population. This study provides basic data for establishing the MRLs and serving as a reference for developing the analytical method applicable to CYA residues in different plant matrices.
Deciphering the Cape Gooseberry Fruits Mycobiome for Further Safety Improvement Postharvest
Authors: Tenea Gabriela N. , Molina Diana
Published: October 2024

Cape gooseberries are exquisitely flavored fruits; their rapid deterioration reduces their shelf life. Understanding the unique mycobiome of fruit peels is an essential step in identifying the taxa causing postharvest loss. The current study proposes to analyze the fungal communities of cape gooseberry peels collected from an organic orchard at unripe and ripe stages and purchased from open-air market sites, using the ITS2 region metabarcoding. According to the Kruskal–Wallis test, there were no statistically significant differences found in either the phylogenetic or non-phylogenetic alpha diversity indices. Significant differences in fungal communities were observed between the market and orchard groups based on beta diversity results. Ascomycota (85.72–96.76%), Basidiomycota (3.21–13.91%), and Chytridiomycota (0.07–9.35%) were the most common fungal phyla, their abundance varying with the ripening stage and origin. Dothideomycetes in the orchard group and Saccharomycetes in the market group were the two most prevalent classes. Furthermore, we investigate which taxa showed a significant difference in abundance between the two conditions (market vs. orchard) using the analysis of compositions of microbiomes with bias correction (ANCOM-BC) test. Regardless of the phase, the orchard samples exhibited a notable increase in the mean absolute abundance of various beneficial fungal taxa, including Tilletiopsis washingtonensis and Articulospora proliferata, whereas the market samples demonstrated a high abundance of harmful yeasts and molds such as Meyerozyma guilliermondii, Candida railenensis, and Botrytis caroliniana. Although it is unclear how these microorganisms augment at the market sites and might impact the fruit quality after harvest, from a fruit safety perspective, it is essential to comprehend the diversity and variation of the mycobiome composition at different ripening stages to further develop strategies to improve food safety postharvest.
A Study of the Influence of Ion-Ozonized Water on the Properties of Pasta Dough Made from Wheat Flour and Pumpkin Powder
Authors: Iztayev Bauyrzhan , Iztayev Auyelbek , Kulazhanov Talgat , Iskakova Galiya , Yakiyayeva Madina , Muldabekova Bayan , Baiysbayeva Meruyet , Tursunbayeva Sholpan
Published: October 2024

Water treated with ion ozone improves the technological qualities of food products. Therefore, ion-ozonated water was used in the work, and whole-grain flour from soft wheat of the Almaly variety and pumpkin powder were used as raw materials to improve the quality and nutritional value of the pasta. This study investigated the effects of ion-ozone concentration in ion-ozonated water Cio, water temperature tw, pumpkin powder content Cpp and drying temperature td on various characteristics affecting the quality of pasta, including its organoleptic physical, chemical, and rheological properties. These characteristics were assessed by conducting multiple experiments, a total of 25 indicators were determined, such as humidity, acidity, cooking properties, deformation, and other basic quality indicators. To reduce the number of experiments and obtain a reliable assessment of the influence of individual factors on the quality indicators of pasta, methods involving the multifactorial design of experiments were applied. Data processing and all necessary calculations were carried out using the PLAN sequential regression analysis program. Consequently, our findings indicate that minimizing dry water (DM) loss in cooking water requires a dual approach: increasing ion-ozone concentration and optimizing pasta composition and drying conditions, specifically by reducing pumpkin powder content and drying temperature. As a result, it was established that to obtain high-quality pasta from whole-grain flour with high quality and rheological properties, it is necessary to use the following optimal production modes: ion-ozone concentration in ion-ozonated water Cio = 2.5 × 10−6 mg/cm3, water temperature tw = 50 °C, pumpkin powder content Cpp = 3.0%, and pasta drying temperature td = 50 °C. The resulting pasta is an environmentally friendly product with a high content of biologically active substances.
Antibiotic Resistance Genes in Global Food Transformation System: Edible Insects vs. Livestock
Authors: Raka Rifat Nowshin , Zhang Lin , Chen Rui , Xue Xiaofeng
Published: October 2024

Antibiotic-resistant genes (ARGs) pose a significant threat to the global food transformation system. The increasing prevalence of ARGs in food has elicited apprehension about public health safety. The widespread dissemination of ARGs in food products, driven by the inappropriate use of antibiotics, presents significant adversity for the safety of emerging future food sources such as edible insects. As the world faces increasing challenges related to food security, climate change, and antibiotic resistance, edible insects offer a sustainable and resilient food source. The intriguing possibility of edible insects serving as a less conducive environment for ARGs compared to livestock warrants further exploration and investigation. In this recent work, we listed ARGs from edible insects detected so far by in vitro approaches and aimed to construct a fair comparison with ARGs from livestock based on relevant genes. We also presented our argument by analyzing the factors that might be responsible for ARG abundance in livestock vs. edible insects. Livestock and edible insects have diverse gut microbiota, and their diets differ with antibiotics. Consequently, their ARG abundance may vary as well. In addition, processed edible insects have lower levels of ARGs than raw ones. We hypothesize that edible insects could potentially contain a lower abundance of ARGs and exhibit a diminished ability to disseminate ARGs relative to livestock. A regulatory framework could help intercept the increasing prevalence of ARGs. Due diligence should also be taken when marketing edible insects for consumption.
The Protective Effect against Lung Injury of Phytosome Containing the Extract of Purple Waxy Corn Tassel in an Animal Model of PM2.5-Induced Lung Inflammation
Authors: Palachai Nut , Thukham-mee Wipawee , Wattanathorn Jintanaporn
Published: October 2024

Lung inflammation caused by fine particulate matter (PM), particularly PM2.5, poses a significant public health challenge, with oxidative stress and inflammation playing central roles in its pathophysiology. This study evaluates the protective effects of phytosome-encapsulated extract of purple waxy corn tassel (PPT) against PM2.5-induced lung inflammation. Male Wistar rats received PPT at doses of 100, 200, and 400 mg/kg BW for 21 days prior to exposure and continued to receive the same doses for 27 days during PM2.5 exposure. Significant reductions in inflammatory markers, including cyclooxygenase-2 (COX-II), various interleukins (IL-1β, IL-6, IL-8), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa B (NF-κB), were observed, indicating that PPT effectively regulates the inflammatory response. Additionally, PPT improved oxidative stress markers by reducing malondialdehyde (MDA) levels and enhancing antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), thereby restoring lung antioxidant defenses. Notably, the study revealed that PPT modulates epigenetic mechanisms, as evidenced by decreased histone deacetylase (HDAC) activity and upregulation of sirtuins in lung tissue. These epigenetic modifications likely contribute to the reduction in inflammation and oxidative stress, suggesting a multifaceted protective role of PPT that involves both direct biochemical pathways and epigenetic regulation. The interplay between reduced inflammatory signaling, enhanced antioxidant capacity, and epigenetic modulation underscores PPT’s potential as a therapeutic agent for managing respiratory inflammation-related diseases and its promise for the development of future functional food products.
Effects of Temperature and Packaging Atmosphere on Shelf Life, Biochemical, and Sensory Attributes of Glasswort (Salicornia europaea L.) Grown Hydroponically at Different Salinity Levels
Authors: Sanmartin Chiara , Taglieri Isabella , Bianchi Alessandro , Parichanon Prangthip , Puccinelli Martina , Pardossi Alberto , Venturi Francesca
Published: October 2024

Halophytes, such as Salicornia species, are promising new foods and are consumed for their pleasant salty taste and nutritional value. Since Salicornia is perishable, modified atmospheric packaging (MAP) can be a useful tool, in combination with proper temperature, to halt further quality degradation in this type of product. The purpose of this study was to investigate the effect of MAP, with or without refrigeration, to extend the shelf life of glasswort (Salicornia europaea L.) grown hydroponically (floating raft system) in a greenhouse with a nutrient solution containing 0 g/L (C) or 12.5 g/L of NaCl (T). The dry matter content, weight loss, respiration rate, biochemical composition, color, antioxidant capacity, and sensorial attributes were determined in shoots after harvest and during storage in plastic bags filled with technical air or with MAP at 4 or 20 °C for 120 h. At harvest, plants supplied with salt-enriched solution (T) showed a significant improvement in nutritional value and sensory profile. Storage in air at room temperature (20 °C) accelerated weight loss and diminished color stability, particularly in non-salinity samples (C), while MAP extended the shelf life of all the samples regardless of the storage temperature adopted. Optimal storage conditions were observed when MAP was combined with refrigeration, which allowed to effectively preserve shoots sensory acceptability for a period of about seven days. Future research could further explore the long-term effects on the nutritional value and sensory quality of S. europaea under various combinations of MAP and different storage temperatures ranging between 4 °C and 20 °C.
Preservative Effect of Alga Flour Extract on Frozen Horse Mackerel (Trachurus trachurus) Lipids
Authors: Martínez Miriam , Trigo Marcos , Aubourg Santiago P. , Rodríguez Alicia
Published: October 2024

The aim of this study was to investigate the preservative properties of alga Gelidium sp. flour when included in the glazing medium employed for the frozen storage (−18 °C) of horse mackerel (Trachurus trachurus). Different concentrations (low, medium, and high) of an aqueous extract were tested and compared to a control water-glazing condition. Quality changes (lipid oxidation and hydrolysis, fatty acid (FA) profile, and trimethylamine (TMA) formation) were determined after 3- and 6-month storage periods. A general quality loss (lipid oxidation with hydrolysis development and TMA formation) with the frozen storage period was detected in all samples. The presence of an alga flour (AF) extract in the glazing medium led to a lower (p < 0.05) TBARS and fluorescent compound formation and to higher (p < 0.05) polyene values in frozen fish. Furthermore, a preserving effect on free fatty acids was detected in AF-treated fish. On the contrary, the AF-glazing treatment did not affect (p > 0.05) the TMA formation and the total n3/total n6 FA ratio. In general, preservative effects were found to be higher in frozen fish corresponding to the medium concentration tested. Current results show the potential of Gelidium sp. flour as a natural source of preservative hydrophilic compounds for the quality enhancement of frozen horse mackerel.
Characterization of the Bacillus cereus Group Isolated from Ready-to-Eat Foods in Poland by Whole-Genome Sequencing
Authors: Kowalska Joanna , Maćkiw Elżbieta , Korsak Dorota , Postupolski Jacek
Published: October 2024

Bacillus cereus sensu lato can contaminate food and cause food poisoning by producing toxins such as cereulide, toxin BL, and cytotoxin K. In this study, we retrospectively analyzed B. cereus sensu lato from retail food products and food poisoning cases using PCR methods to determine their virulence profiles. A new toxin profile, encoding all four toxins (hbl, nhe, cytK, ces), was found in 0.4% of isolates. The toxin profiles, classified into A-J, revealed that 91.8% harbored nhe genes, while hbl, cytK, and ces were detected in 43.8%, 46.9%, and 4.2% of isolates, respectively. Whole-genome sequencing (WGS) identified four distinct species within the B. cereus group, with 21 isolates closely related to B. cereus sensu stricte, 25 to B. mosaicus, 2 to B. toyonensis, and 1 to B. mycoides. Three novel sequence types (STs 3297, 3298, 3299) were discovered. Antibiotic resistance genes were common, with 100% of isolates carrying beta-lactam resistance genes. Fosfomycin (80%), vancomycin (8%), streptothricin (6%), tetracycline (4%), and macrolide resistance (2%) genes were also detected. These results highlight the genetic diversity and antibiotic resistance potential of B. cereus sensu lato strains in Polish food products.
Adivasis as Ecological Warriors: Colonial Laws and Post-Colonial Adivasi Resistance in India’s Jharkhand
Authors: Singh Anjana
Published: October 2024

The growing divide between the capitalist mode of development promoted by the state and the participative development model suggested by the people has brought ecology, environment, and existence to the core of all contemporary debates. The Adivasi (indigenes) who constitute 8.6 percent of the entire population of India are engaged in a constant battle to save their ecology and landscape. Represented as communities whose existence is intertwined with ‘Jal, Jungle, Jameen’ (water, forest, and land), Adivasis are the most prominent communities facing dispossession and displacement from their roots to further the ideology of development in which they have no stake. The notion of Adivasis as ‘savage’, ‘primitive’, and ‘backward’ communities that are incompetent of ‘developing’ themselves, resulting in their ‘backwardness’ gets carried over from the colonial to the contemporary period. Exposed to the processes of mining and industrialisation, Adivasis and their ecological resources have been exploited since the colonial period to suit the development model of the state. The Adivasi notion of selfhood was overlooked in the process of making the areas inhabited by them zones of ‘exclusive governmentality’. The paper argues and analyses this transformation process of Adivasis into ecological warriors; a process in which they used their shared, remembered and lived past to assert their customary rights. Basing the study on three environmental movements of state of Jharkhand in Central India, namely the Koel-Karo movement of the 1980s, the Netarhat movement of the 1990s, and the Pathalgadi movement of 2017–18, the study underlines that the Adivasi of Jharkhand anchored on their customary rights as a weapon, to protect their ecology and landscape against various state-sponsored development schemes. Drawing on the methodology of field investigation, interaction with the NGOs, government reports and media reports, the article argues that these community struggles are rays of hope for a global ecological future.
Psychometric Properties of the World Health Organization Quality of Life Scale for Older Adults (WHO-QoL-Old) in a Mexican Population
Authors: Díaz de León Castañeda Christian , Anguiano-Morán Ana Celia , Valtierra-Oba Elva Rosa , Lemus-Loeza Barbara Monica , Galván-Villalobos Gabriela , Rodríguez-Orozco Alaín Raimundo
Published: October 2024

Background/Objective: The present study aimed to contribute to analyzing the psychometric properties of the WHO Quality of Life Scale for Older Adults (WHO-QoL-Old) in a sample of older adults in Michoacán, Mexico. Methods: 111 older adults from Michoacán, Mexico, participated in the study. Confirmatory factor analysis (CFA) was conducted to test the fit of various models. Data analysis was performed using R Studio, considering the ordinal nature of the items in the model estimation method. Internal consistency was evaluated using the alpha coefficient (α) and McDonald’s omega coefficient (ω). Results: The CFA indicated that the six-correlated-factor model proposed theoretically showed a very good fit (χ2: 397.11, p < 0.001; CFI: 0.958; SRMR: 0.079; RMSEA: 0.079). The factors within the model demonstrated acceptable internal consistency, with an alpha coefficient ranging from 0.739 to 0.874 and an omega coefficient ranging from 0.748 to 0.882. Conclusions: It is concluded that the WHO-QoL-Old scale presents good psychometric properties for the Mexican older adult population.
Burnout and Stress in Forensic Science Jobs: A Systematic Review
Authors: Lombardo Claudia , Capasso Emanuele , Li Rosi Giuseppe , Salerno Monica , Chisari Mario , Esposito Massimiliano , Di Mauro Lucio , Sessa Francesco
Published: October 2024

Background/Objectives. Burnout and occupational stress are significant issues among forensic professionals, impacting their well-being and job performance. This systematic review aims to provide an up-to-date overview of the occupational stress and burnout experienced by forensic personnel, exploring the profound and multifaceted impact on their physical, mental, professional, and interpersonal well-being. Methods. A systematic review was conducted following PRISMA guidelines using Scopus and WOS databases to search for articles published from 1 January 2000 to 31 August 2024. The search used keywords related to burnout and forensic professions. Inclusion criteria were original articles in English and French, while reviews, book chapters, editorials, and notes were excluded. A total of 10 studies were included after eliminating duplicates and excluding irrelevant articles. Results. The review identified seven key findings. (1) High levels of occupational stress and burnout among forensic personnel necessitate effective stress management strategies and resilience training; (2) autopsy technicians in Romania experience burnout and alexithymia, particularly related to traumatic events involving children, highlighting the need for specialized support systems; (3) disparities in burnout and post-traumatic stress disorder (PTSD) symptoms were observed in autopsy technicians and resident doctors, suggesting tailored mental health resources; (4) organizational factors, such as peer support and compensation satisfaction, significantly impact burnout and secondary traumatic stress (STS) among sexual assault nurse examiners; (5) burnout among forensic physicians, both in Romania and Egypt, is linked to personality traits, job satisfaction, and socio-demographic factors; (6) pathologists face a range of health issues, including musculoskeletal problems and psychological disorders, underscoring the need for industry-specific health measures; and (7) the lack of wellness resources for forensic professionals calls for improved mental health support and training. Conclusions. The findings highlight the pervasive issue of burnout and stress among forensic professionals globally. Addressing these challenges requires comprehensive stress management programs, tailored mental health resources, and organizational support. Future research should focus on developing and implementing effective interventions to enhance resilience and job satisfaction within this high-stress field.
Study of the Phenolic Compounds and Biological Activities of the Wild Fruits of Vaccinium leucanthum Schltdl.
Authors: Bernal-Gallardo José Osvaldo , Mena-Violante Hortencia Gabriela , Luna-Suárez Silvia
Published: October 2024

Around 450 species of blueberries of the genus Vaccinium are known, of which some have gained preferential breeding, such as the ‘Biloxi’ variety. Some little studied species, such as Vaccinium leucanthum Schltdl. located in Mexico, could be a potential source of bioactive compounds. In this study, the phenolic compounds (chlorogenic acid content, hyperoside, phenols, flavonoids, tannins and total anthocyanins content) as well as the potential biological activity (antioxidant, antimicrobial, xanthine oxidase converting enzyme inhibition and angiotensin I inhibition) of Vaccinium leucanthum Schltdl. were studied, making a comparison with the Biloxi variety, which is the most widely cultivated one. The extract of V. leucanthum showed the highest content of flavonoids (4.853 ± 0.341 mg QE/g DW), total anthocyanins (0.303 ± 0.008 mg CGE/g DW), petunidin-3-glucoside (6.92 ± 0.12 mg PGE/g DW), malvidin-3-glucoside (11.80 ± 0.10 mg MGE/g DW) and hyperoside (5.137 ± 0.100 mg HE/g DW). It should be noted that V. leucanthum showed the same total tannin content and the same efficacy in the inhibition of Angiotensin I-converting enzyme as ‘Biloxi’, as well as the same antibacterial effect against the enterobacteria Salmonella choleraesuis ATCC 12022, Escherichia coli ATCC 12792and Shigella flexneri ATCC 10708. These findings demonstrate that V. leucanthum extracts could be an important source of preservatives as well as nutraceutical compounds for use in foods and medicines.
Estimation of Reservoir Storage Capacity Using the Gould-Dincer Formula with the Aid of Possibility Theory
Authors: Mylonas Nikos , Tzimopoulos Christos , Papadopoulos Basil , Samarinas Nikiforos
Published: October 2024

This paper presents a method for estimating reservoir storage capacity using the Gould–Dincer normal formula (G-DN), enhanced by the possibility theory. The G-DN equation is valuable for regional studies of reservoir reliability, particularly under climate change scenarios, using regional statistics. However, because the G-DN formula deals with measured data, it introduces a degree of uncertainty and fuzziness that traditional probability theory struggles to address. Possibility theory, an extension of fuzzy set theory, offers a suitable framework for managing this uncertainty and fuzziness. In this study, the G-DN formula is adapted to incorporate fuzzy logic, and the possibilistic nature of reservoir capacity is translated into a probabilistic framework using α-cuts from the possibility theory. These α-cuts approximate probability confidence intervals with high confidence. Applying the proposed methodology, in the present crisp case with the storage capacity D = 0.75, the value of the capacity C was found to be 1271×106 m3, and that for D = 0.5 was 634.5×106 m3. On the other hand, in the fuzzy case using the possibility theory, the value of the capacity for D = 0.75 is the internal [315,5679]×106 m3 and for D = 0.5 the value is interval [158,2839]×106 m3, with a probability of ≥95% and a risk level of α = 5% for both cases. The proposed approach could be used as a robust tool in the toolkit of engineers working on irrigation, drainage, and water resource projects, supporting informed and effective engineering decisions.
Executive Green Perception and Green Innovation Improve New Quality Productivity in Chinese Listed Firms
Authors: Li Jiaran , Ibrahim Haslindar
Published: October 2024

This research focuses on Chinese listed companies to explore the influence of green-oriented strategies and green innovation on corporate productivity. Using empirical data from 2011 to 2022, the study investigates the positive effects of executives’ green perceptions on new quality productivity and the enhancing role of green innovation. The results indicate that executives’ green perceptions significantly enhance new quality productivity. Furthermore, heterogeneity analyses reveal variations in this effect based on firm size, type, and pollution levels, demonstrating the environmental sensitivity of green strategies. Robustness tests reinforce the consistency of these results. Additionally, the research establishes that green innovation not only directly boosts new quality productivity but also strengthens the positive influence of executive green perceptions on productivity. These insights emphasize the critical synergy between green innovation and executive commitment to sustainability as a means to boost productivity, offering valuable guidance for policymakers and business leaders aiming to advance corporate productivity through sustainable practices.
Proton Radiation Therapy: A Systematic Review of Treatment-Related Side Effects and Toxicities
Authors: Salem Peter P. , Chami Perla , Daou Remy , Hajj Joseph , Lin Haibo , Chhabra Arpit M. , Simone Charles B. , Lee Nancy Y. , Hajj Carla
Published: October 2024

Cancer is the second leading cause of death worldwide. Around half of all cancer patients undergo some type of radiation therapy throughout the course of their treatment. Photon radiation remains (RT) the most widely utilized modality of radiotherapy despite recent advancements in proton radiation therapy (PBT). PBT makes use of the particle’s biological property known as the Bragg peak to better spare healthy tissue from radiation damage, with data to support that this treatment modality is less toxic than photon RT. Hence, proton radiation dosimetry looks better compared to photon dosimetry; however, due to proton-specific uncertainties, unexpected acute, subacute, and long-term toxicities can be encountered. Reported neurotoxicity resulting from proton radiation treatments include radiation necrosis, moyamoya syndrome, neurosensory toxicities, brain edema, neuromuscular toxicities, and neurocognitive toxicities. Pulmonary toxicities include pneumonitis and fibrosis, pleural effusions, and bronchial toxicities. Pericarditis, pericardial effusions, and atrial fibrillations are among the cardiac toxicities related to proton therapy. Gastrointestinal and hematological toxicities are also found in the literature. Genitourinary toxicities include urinary and reproductive-related toxicities. Osteological, oral, endocrine, and skin toxicities have also been reported. The side effects will be comparable to the ones following photon RT, nonetheless at an expected lower incidence. The toxicities collected mainly from case reports and clinical trials are described based on the organs affected and functions altered.
Membrane Ruffles: Composition, Function, Formation and Visualization
Authors: Yan Guiqin , Zhou Jie , Yin Jiaxin , Gao Duolan , Zhong Xiaohai , Deng Xiaoyan , Kang Hongyan , Sun Anqiang
Published: October 2024

Membrane ruffles are cell actin-based membrane protrusions that have distinct structural characteristics. Linear ruffles with columnar spike-like and veil-like structures assemble at the leading edge of cell membranes. Circular dorsal ruffles (CDRs) have no supporting columnar structures but their veil-like structures, connecting from end to end, present an enclosed ring-shaped circular outline. Membrane ruffles are involved in multiple cell functions such as cell motility, macropinocytosis, receptor internalization, fluid viscosity sensing in a two-dimensional culture environment, and protecting cells from death in response to physiologically compressive loads. Herein, we review the state-of-the-art knowledge on membrane ruffle structure and function, the growth factor-induced membrane ruffling process, and the growth factor-independent ruffling mode triggered by calcium and other stimulating factors, together with the respective underlying mechanisms. We also summarize the inhibitors used in ruffle formation studies and their specificity. In the last part, an overview is given of the various techniques in which the membrane ruffles have been visualized up to now.
Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
Authors: Vilà-Quintana Laura , Fort Esther , Pardo Laura , Albiol-Quer Maria T. , Ortiz Maria Rosa , Capdevila Montserrat , Feliu Anna , Bahí Anna , Llirós Marc , García-Velasco Adelaida , Morell Ginestà Mireia , Laquente Berta , Pozas Débora , Moreno Victor , Garcia-Gil Librado Jesús , Duell Eric Jeffrey , Pimenoff Ville Nikolai , Carreras-Torres Robert , Aldeguer Xavier
Published: October 2024

Individuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and saliva samples obtained from patients with chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), and healthy controls (HCs).We observed similar microbiota composition at the species level in both the gut and oral samples in PDAC patients compared to HCs, among which the most distinctive finding was that the abundance of oral-originated Fusobacterium nucleatum species did not differ between the oral and the gut samples. Moreover, comparing PDAC patients with HCs, Klebsiella oxytoca was significantly more abundant in the stool samples of PDAC patients, while Streptococcus spp. showed higher abundance in both the oral and stool samples of PDAC patients. Finally, the most important finding was the distinctive gut phage–bacterial interactome pattern among PDAC patients. CrAssphages, particularly Blohavirus, showed mutual exclusion with K. oxytoca species, while Burzaovirus showed co-occurrence with Enterobacteriaceae spp., which have been shown to be capable of inducing DNA damage in human pancreatic cells ex vivo. The interactome findings warrant further mechanistic studies, as our findings may provide new insights into developing microbiota-based diagnostic and therapeutic methods for pancreatic diseases.
Systematic Review of Peptide CAQK: Properties, Applications, and Outcomes
Authors: Castillo Jose A. , Le Michael Nhien , Ratcliff Amanda , Soufi Khadija , Huang Kuanwei , Vatoofy Sina , Ghaffari-Rafi Arash , Emerson Samuel , Reynolds Elizabeth , Pivetti Christopher , Clark Kaitlin , Martin Allan , Price Richard , Kim Kee , Wang Aijun , Russo Rachel
Published: October 2024

Many central nervous system (CNS) disorders lack approved treatment options. Previous research demonstrated that peptide CAQK can bind to chondroitin sulfate proteoglycans (CSPGs) in the extracellular matrix of the CNS. In vivo studies have investigated CAQK conjugated to nanoparticles containing therapeutic agents with varying methodologies/outcomes. This paper presents the first systematic review assessing its properties, applications, and outcomes secondary to its use. Following PRISMA guidelines, a comprehensive search was performed across multiple databases. Studies utilizing CAQK as a therapeutic agent/homing molecule in animal/human models were selected. Sixteen studies met the inclusion criteria. Mice and rats were the predominant animal models. All studies except one used CAQK to deliver a therapeutic agent. The reviewed studies mostly included models of brain and spinal cord injuries. Most studies had intravenous administration of CAQK. All studies demonstrated various benefits and that CAQK conjugation facilitated localization to target tissues. No studies directly evaluated the effects of CAQK alone. The data are limited by the heterogeneity in study methodologies and the lack of direct comparison between CAQK and conjugated agents. Overall, these findings present CAQK utilization to deliver a therapeutic agent as a promising targeting strategy in the management of disorders where CSPGs are upregulated.
Epigenetic Modifications Are Involved in Transgenerational Inheritance of Cadmium Reproductive Toxicity in Mouse Oocytes
Authors: Zhu Jiaqiao , Guo Shuai , Cao Jiangqin , Zhao Hangbin , Ma Yonggang , Zou Hui , Ju Huiming , Liu Zongping , Li Junwei
Published: October 2024

Maternal cadmium exposure during pregnancy has been demonstrated to have detrimental effects on offspring development. However, the impact of maternal cadmium exposure on offspring oocytes remains largely unknown, and the underlying mechanisms are not fully understood. In this study, we found that maternal cadmium exposure during pregnancy resulted in selective alteration in epigenetic modifications of mouse oocytes in offspring, including a decrease in H3K4me2 and H4K12ac, as well as an increase in DNA methylation of H19. Although ROS levels and mitochondrial activity remain at normal levels, the DNA damage marker γH2AX was significantly increased and the DNA repair marker DNA-PKcs was remarkably decreased in offspring oocytes from maternal cadmium exposure. These alterations are responsible for the decrease in the quality of mouse oocytes in offspring induced by maternal cadmium exposure. As a result, the meiotic maturation of oocytes and subsequent early embryonic development are influenced by maternal cadmium exposure. RNA-seq results showed that maternal cadmium exposure elicits modifications in the expression of genes associated with metabolism, signal transduction, and endocrine regulation in offspring ovaries, which also contribute to the disorders of oocyte maturation and failures in early embryonic development. Our research provides direct evidence of transgenerational epigenetic inheritance of cadmium reproductive toxicity in mouse germ cells.