bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationships between Structural Indices and Conventional Stand Attributes in an Old-Growth Forest in Southeast Europe
Authors: Keren Srđan , Svoboda Miroslav , Janda Pavel , Nagel Thomas A.
Published: December 2019

Structural indices are often proposed as guiding measures for increasing structural heterogeneity. However, few studies have examined the association between such indices and conventional stand attributes. The primary objectives of this study were to evaluate changes in structural heterogeneity and tree species diversity at different plot sizes and to quantify the relationships between conventional stand attributes (mean tree diameter, absolute tree density, basal area, species proportion) and structural indices in a mixed old-growth forest in Southeast Europe. Paired tests were used to identify significant changes in structural heterogeneity with increased plot area, while the relationships between stand attributes and analyzed indices (Gini, diameter differentiation, species mingling, and Shannon’s index) were evaluated with Pearson’s correlations. The index values of Gini, diameter differentiation, and tree species mingling were rather stable with the increase of plot size, whereas tree species diversity increased significantly with the increase of plot area from 200 m2 to 1500 m2. The measures of tree species mingling and tree species diversity were strongly associated with each other, while their association with diameter variability was weak to moderately strong. Tree species mingling index was strongly associated with the changes in tree species proportions. However, conventional stand attributes were generally not strongly correlated with the examined indices. For restoring and maintaining old-growth characteristics, forest managers may use structural indices to increase small-scale structural heterogeneity, tree species mingling, and diversity, but only as an additional set of measures, not as surrogates for conventional stand attributes.
Prediction of the Long-Term Potential Distribution of Cryptorhynchus lapathi (L.) under Climate Change
Authors: Zou Ya , Zhang Linjing , Ge Xuezhen , Guo Siwei , Li Xue , Chen Linghong , Wang Tao , Zong Shixiang
Published: December 2019

The poplar and willow borer, Cryptorhynchus lapathi (L.), is a severe worldwide quarantine pest that causes great economic, social, and ecological damage in Europe, North America, and Asia. CLIMEX4.0.0 was used to study the likely impact of climate change on the potential global distribution of C. lapathi based on existing (1987–2016) and predicted (2021–2040, 2041–2080, and 2081–2100) climate data. Future climate data were simulated based on global climate models from Coupled Model Inter-comparison Project Phase 5 (CMIP5) under the RCP4.5 projection. The potential distribution of C. lapathi under historical climate conditions mainly includes North America, Africa, Europe, and Asia. Future global warming may cause a northward shift in the northern boundary of potential distribution. The total suitable area would increase by 2080–2100. Additionally, climatic suitability would change in large regions of the northern hemisphere and decrease in a small region of the southern hemisphere. The projected potential distribution will help determine the impacts of climate change and identify areas at risk of pest invasion in the future. In turn, this will help design and implement effective prevention measures for expanding pest populations, using natural enemies, microorganisms, and physical barriers in very favorable regions to impede the movement and oviposition of C. lapathi.
Screening and Application of Cyberlindnera Yeasts to Produce a Fruity, Non-Alcoholic Beer
Authors: Bellut Konstantin , Michel Maximilian , Zarnkow Martin , Hutzler Mathias , Jacob Fritz , Atzler Jonas J. , Hoehnel Andrea , Lynch Kieran M. , Arendt Elke K.
Published: December 2019

Non-alcoholic beer (NAB) is enjoying growing demand and popularity due to consumer lifestyle trends and improved production methods. In recent years in particular, research into the application of non-Saccharomyces yeasts to produce NAB via limited fermentation has gained momentum. Non-Saccharomyces yeasts are known to produce fruity aromas, owing to a high ester production. This trait could be harnessed to mask the often-criticized wort-like off-flavor of NAB produced via limited fermentation. Six Cyberlindnera strains were characterized and screened in wort extract. Four of the six strains produced a pleasant, fruity aroma while exhibiting low ethanol production. The strain Cyberlindnera subsufficiens C6.1 was chosen for fermentation optimization via response surface methodology (RSM) and a pilot-scale (60 L) brewing trial with subsequent sensory evaluation. A low fermentation temperature and low pitching rate enhanced the fruitiness and overall acceptance of the NAB. The NAB (0.36% ABV) produced on pilot-scale was significantly more fruity and exhibited a significantly reduced wort-like off-flavor compared to two commercial NABs. This study demonstrated the suitability of Cyberlindnera subsufficiens to produce a fruity NAB, which can compete with commercial NABs. The outcome strengthens the position of non-Saccharomyces yeasts as a serious and applicable alternative to established methods in NAB brewing.
Seabream Larval Physiology under Ocean Warming and Acidification
Authors: Pimentel Marta S. , Faleiro Filipa , Machado Jorge , Pousão-Ferreira Pedro , Rosa Rui
Published: December 2019

The vulnerability of early fish stages represents a critical bottleneck for fish recruitment; therefore, it is essential to understand how climate change affects their physiology for more sustainable management of fisheries. Here, we investigated the effects of warming (OW; +4 °C) and acidification (OA; ΔpH = 0.5) on the heart and oxygen consumption rates, metabolic enzymatic machinery—namely citrate synthase (CS), lactate dehydrogenase (LDH), and ß-hydroxyacyl CoA dehydrogenase (HOAD), of seabream (Sparus aurata) larvae (fifteen days after hatch). Oxygen consumption and heart rates showed a significant increase with rising temperature, but decreased with pCO2. Results revealed a significant increase of LDH activity with OW and a significant decrease of the aerobic potential (CS and HOAD activity) of larvae with OA. In contrast, under OA, the activity levels of the enzyme LDH and the LDH:CS ratio indicated an enhancement of anaerobic pathways. Although such a short-term metabolic strategy may eventually sustain the basic costs of maintenance, it might not be adequate under the future chronic ocean conditions. Given that the potential for adaptation to new forthcoming conditions is yet experimentally unaccounted for this species, future research is essential to accurately predict the physiological performance of this commercially important species under future ocean conditions.
The Influence of Different Smoking Procedures on the Content of 16 PAHs in Traditional Dry Cured Smoked Meat “Hercegovačka Pečenica”
Authors: Puljić Leona , Mastanjević Krešimir , Kartalović Brankica , Kovačević Dragan , Vranešević Jelena , Mastanjević Kristina
Published: December 2019

During smoking, meat products may get contaminated by polycyclic aromatic hydrocarbons (PAH), especially the ones that are smoked in traditional (uncontrolled) conditions. This study aims to evaluate the difference in PAH content in samples of traditional dry cured pork meat products, “Hercegovačka pečenica”, produced in (1) a traditional smokehouse and (2) in industrial chambers. The study revealed that the content of the four priority PAHs (PAH4) in samples produced in a traditional smoking manner highly exceeded (up to 10 times) the maximal limits set for PAHs (12 µg/kg). PAH4 in all samples subjected to industrial smoking procedures was below the limit of quantification. All samples had below-the-limit-of-quantification values for Benzo[a]pyrene. The surface layer of the samples produced in traditional conditions had the highest total content of PAH16. The inner parts of all samples, whether traditional or industrial, had significantly lower PAH16 concentration than the surface layer.
Gendered Spaces Captured in Cultural Representations: Conceptualising the Indian Experience in Arundhati Roy’s The Ministry of Utmost Happiness
Authors: Gopinath Swapna
Published: December 2019

Spatiality has emerged as a significant component in analyzing gendered experiences, and cultural expressions reveal this complex yet dynamic relationship in several ways. While some forms of art approach it in a direct, straightforward manner, literature does it, perhaps, in aesthetically diverse ways. Arundhati Roy has foregrounded the space and gender relationship in several ways, with language emerging as the most intricate tool to depict this relationship. Her second and latest novel, The Ministry of Utmost Happiness (2017) is a novel that has space as a prominent character and the gender identities, depicted in this novel, are challenging and interesting, especially in the context of modern India. From the transgender identity of the protagonist who resides in the heterotopic space of a cemetery to the female characters who sustain themselves in the conflict-ridden Kashmir valley, the novel experiments with possibilities using linguistic styles as the most appropriate and significant tool. Roy’s experiments with form, with regards to gendered spatial experiences, will be explored in this paper. I will work within the framework of thirdspace and heterotopia as postulated by Soja and Foucault. This paper will analyse the depiction of gender within social spaces using the tools of Cultural Studies.
An Approach for the Analysis of the Accessibility of Fire Hydrants in Urban Territories
Authors: Raškauskaitė Raminta , Grigonis Vytautas
Published: December 2019

Globally, fire causes considerable losses that can be alleviated by taking appropriate actions facilitated by systems supported by geo-information technologies. This research focuses upon the development of an approach for planning urban infrastructures, and particularly in the accessibility of fire hydrants. Accessibility of fire hydrants’ infrastructure in urban territories is one of the key elements in fire risk management and public safety. The main result of the research is a comprehensive and structured Geographic Information Systems (GISs)-based dataset for the fast and more efficient planning of fire hydrants in urban territories. The proposed framework for data collection and processing was used to determine the distribution of hydrants, location of fire brigade stations and areas and to demonstrate the capabilities of the existing municipal fire extinguishing systems in Vilnius City, Lithuania. Later on, research on fire hydrants’ accessibility, analysis of the location of protected and unprotected urban territories and marking of unprotected buildings, was carried out. The resulting map of unprotected urban territories can be of great benefit for understanding fire risks and offering more effective ways for fire risk management.
A Semantic Expansion Model for VGI Retrieval
Authors: Sun Tao , Xia Hui , Li Lin , Shen Hang , Liu Yu
Published: December 2019

OpenStreetMap (OSM) is a representative volunteered geographic information (VGI) project. However, there have been difficulties in retrieving spatial information from OSM. Ontology is an effective knowledge organization and representation method that is often used to enrich the search capabilities of search systems. This paper constructed an OSM ontology model with semantic property items. A query expansion method is also proposed based on the similarity of properties of the ontology model. Moreover, a relevant experiment is conducted using OSM data related to China. The experimental results demonstrate that the recall and precision of the proposed method reach 80% and 87% for geographic information retrieval, respectively. This study provides a method that can be used as a reference for subsequent research on spatial information retrieval.
What Went Wrong for Bad Solvers during Thematic Map Analysis? Lessons Learned from an Eye-Tracking Study
Authors: Havelková Lenka , Gołębiowska Izabela Małgorzata
Published: December 2019

Thematic map analysis is a complex and challenging task that might result in map user failure for many reasons. In the study reported here, we wanted to search for differences between successful and unsuccessful map users, focusing—unlike many similar studies—on strategies applied by users who give incorrect answers. In the eye-tracking study, followed by a questionnaire survey, we collected data from 39 participants. The eye-tracking data were analyzed both qualitatively and quantitatively to compare participants’ strategies from various perspectives. Unlike the results of some other studies, it turned out that unsuccessful participants show some similarities that are consistent across most analyzed tasks. The main issues that characterize bad solvers relate to improper use of the thematic legend, the inability to focus on relevant map layout elements, as well as on adequate map content. Moreover, they differed in the general problem-solving approach used as they, for example, tended to choose fast, less cautious, strategies. Based on the collected results, we developed tips that could help prevent unsuccessful participants ending with an incorrect answer and therefore be beneficial in map use education.
“Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
Authors: Pluhařová Kamila , Leontovyčová Hana , Stoudková Věra , Pospíchalová Romana , Maršík Petr , Klouček Pavel , Starodubtseva Anastasiia , Iakovenko Oksana , Krčková Zuzana , Valentová Olga , Burketová Lenka , Janda Martin , Kalachova Tetiana
Published: December 2019

The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its role is best described in the context of plant response to pathogen attack. During infection, SA is rapidly accumulated throughout the green tissues and is important for both local and systemic defences. However, some genetic/metabolic variations can also result in SA overaccumulation in plants, even in basal conditions. To date, more than forty Arabidopsis thaliana mutants have been described as having enhanced endogenous SA levels or constitutively activated SA signalling pathways. In this study, we established a collection of mutants containing different SA levels due to diverse genetic modifications and distinct gene functions. We chose prototypic SA-overaccumulators (SA-OAs), such as bon1-1, but also “non-typical” ones such as exo70b1-1; the selection of OA is accompanied by their crosses with SA-deficient lines. Here, we extensively studied the plant development and SA level/signalling under various growth conditions in soil and in vitro, and showed a strong negative correlation between rosette size, SA content and PR1/ICS1 transcript signature. SA-OAs (namely cpr5, acd6, bon1-1, fah1/fah2 and pi4kβ1β2) had bigger rosettes under high light conditions, whereas WT plants did not. Our data provide new insights clarifying a link between SA and plant behaviour under environmental stresses. The presented SA mutant collection is thus a suitable tool to shed light on the mechanisms underlying trade-offs between growth and defence in plants.
Pharmacological Stimulation of Nurr1 Promotes Cell Cycle Progression in Adult Hippocampal Neural Stem Cells
Authors: Moon Haena , Jeon Seong Gak , Kim Jin-il , Kim Hyeon soo , Lee Sangho , Kim Dongok , Park Seungjoon , Moon Minho , Chung Hyunju
Published: December 2019

Nuclear receptor related-1 (Nurr1) protein performs a crucial role in hippocampal neural stem cell (hNSC) development as well as cognitive functions. We previously demonstrated that the pharmacological stimulation of Nurr1 by amodiaquine (AQ) promotes spatial memory by enhancing adult hippocampal neurogenesis. However, the role of Nurr1 in the cell cycle regulation of the adult hippocampus has not been investigated. This study aimed to examine changes in the cell cycle-related molecules involved in adult hippocampal neurogenesis induced by Nurr1 pharmacological stimulation. Fluorescence-activated cell sorting (FACS) analysis showed that AQ improved the progression of cell cycle from G0/G1 to S phase in a dose-dependent manner, and MEK1 or PI3K inhibitors attenuated this progression. In addition, AQ treatment increased the expression of cell proliferation markers MCM5 and PCNA, and transcription factor E2F1. Furthermore, pharmacological stimulation of Nurr1 by AQ increased the expression levels of positive cell cycle regulators such as cyclin A and cyclin-dependent kinases (CDK) 2. In contrast, levels of CDK inhibitors p27KIP1 and p57KIP2 were reduced upon treatment with AQ. Similar to the in vitro results, RT-qPCR analysis of AQ-administered mice brains revealed an increase in the levels of markers of cell cycle progression, PCNA, MCM5, and Cdc25a. Finally, AQ administration resulted in decreased p27KIP1 and increased CDK2 levels in the dentate gyrus of the mouse hippocampus, as quantified immunohistochemically. Our results demonstrate that the pharmacological stimulation of Nurr1 in adult hNSCs by AQ promotes the cell cycle by modulating cell cycle-related molecules.
Maternal Dietary Fiber Composition during Gestation Induces Changes in Offspring Antioxidative Capacity, Inflammatory Response, and Gut Microbiota in a Sow Model
Authors: Li Yang , Liu Haoyu , Zhang Lijia , Yang Yi , Lin Yan , Zhuo Yong , Fang Zhengfeng , Che Lianqiang , Feng Bin , Xu Shengyu , Li Jian , Wu De
Published: December 2019

To study the effects of maternal dietary fiber composition during gestation on offspring antioxidant capacity, inflammation, and gut microbiota composition, we randomly assigned 64 gilts to four treatments and administered diets with an insoluble/soluble fiber ratio of 3.89 (R1), 5.59 (R2), 9.12 (R3), and 12.81 (R4). Sow samples (blood and feces at gestation 110) and neonatal samples (blood, liver, and colonic contents) were collected. The results showed that sows and piglets in R1 and R2 had higher antioxidant enzyme activity and lower pro-inflammatory factor levels than those in R3 and R4. Moreover, piglets in R1 and R2 had higher liver mRNA expression of Nrf2 and HO-1 and lower NF-κB than piglets in R4. Interestingly, maternal fiber composition not only affected the production of short-chain fatty acids (SCFAs) in sow feces but also influenced the concentrations of SCFAs in the neonatal colon. Results of high-throughput sequencing showed that piglets as well as sows in R1 and R2 had microbial community structures distinct from those in R3 and R4. Therefore, the composition of dietary fiber in pregnancy diet had an important role in improving antioxidant capacity and decreasing inflammatory response of mothers and their offspring through modulating the composition of gut microbiota.
Sphingolipids in Non-Alcoholic Fatty Liver Disease and Hepatocellular Carcinoma: Ceramide Turnover
Authors: Simon Jorge , Ouro Alberto , Ala-Ibanibo Lolia , Presa Natalia , Delgado Teresa Cardoso , Martínez-Chantar María Luz
Published: December 2019

Non-alcoholic fatty liver disease (NAFLD) has emerged as one of the main causes of chronic liver disease worldwide. NAFLD comprises a group of conditions characterized by the accumulation of hepatic lipids that can eventually lead to non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and hepatocellular carcinoma (HCC), the fifth most common cancer type with a poor survival rate. In this context, several works have pointed out perturbations in lipid metabolism and, particularly, changes in bioactive sphingolipids, as a hallmark of NAFLD and derived HCC. In the present work, we have reviewed existing literature about sphingolipids and the development of NAFLD and NAFLD-derived HCC. During metabolic syndrome, considered a risk factor for steatosis development, an increase in ceramide and sphigosine-1-phosphate (S1P) have been reported. Likewise, other reports have highlighted that increased sphingomyelin and ceramide content is observed during steatosis and NASH. Ceramide also plays a role in liver fibrosis and cirrhosis, acting synergistically with S1P. Finally, during HCC, metabolic fluxes are redirected to reduce cellular ceramide levels whilst increasing S1P to support tumor growth.
Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA
Authors: Hu Ruibin , Chen Yi
Published: December 2019

MicroRNAs (miRNAs) are new potential biomarkers for early diagnosis and classification of cancer. This study is the first attempt to use biocatalytic amplification reactions combined with capillary electrophoresis to detect multiple miRNAs simultaneously. In this way, miRNAs, as catalysts, can catalyze two single strands of DNA to form double-strand DNA. Feasibility was demonstrated by non-gel capillary electrophoresis coupled with UV detection (NGCE-UV). The detection limit was improved down to 1.0 nM, having ca. 103-fold improvement. This method has a good linear range of between 3.0 nM and 300 nM, with R2 at 0.99, recovery at 88–115%, and peak area precision at 1–12.7%. Using three target miRNAs as a model can achieve the baseline separation and good selectivity. The proposed biocatalysis coupled with a capillary electrophoresis-based method is simple, rapid, multiplexed, and cost-effective, making it potentially applicable for simultaneous, large-scale screening for other nucleic acids biomarkers and related research.
Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Authors: Yoo Yeon-Jee , Perinpanayagam Hiran , Lee Jue-Yeon , Oh Soram , Gu Yu , Kim A-Reum , Chang Seok-Woo , Baek Seung-Ho , Kum Kee-Yeon
Published: December 2019

Human β defensin-3-C15, an epithelium-derived cationic peptide that has antibacterial/antifungal and immuno-regulatory properties, is getting attention as potential therapeutic agent in endodontics. This study aimed to investigate if synthetic human β defensin-3-C15 (HBD3-C15) peptides could inhibit inflammatory responses in human dental pulp cells (hDPCs), which had been induced by gram-positive endodontic pathogen. hDPC explant cultures were stimulated with Streptococcus gordonii lipoprotein extracts for 24 h to induce expression of pro-inflammatory mediators. The cells were then treated with either HBD3-C15 (50 μg/mL) or calcium hydroxide (CH, 100 μg/mL) as control for seven days, to assess their anti-inflammatory effects. Quantitative RT-PCR analyses and multiplex assays showed that S. gordonii lipoprotein induced the inflammatory reaction in hDPCs. There was a significant reduction of IL-8 and MCP-1 within 24 h of treatment with either CH or HBD3-C15 (p < 0.05), which was sustained over 1 week of treatment. Alleviation of inflammation in both medications was related to COX-2 expression and PGE2 secretion (p < 0.05), rather than TLR2 changes (p > 0.05). These findings demonstrate comparable effects of CH and HDB3-C15 as therapeutic agents for inflamed hDPCs.
Current Projection Methods-Induced Biases at Subgroup Detection for Machine-Learning Based Data-Analysis of Biomedical Data
Authors: Lötsch Jörn , Ultsch Alfred
Published: December 2019

Advances in flow cytometry enable the acquisition of large and high-dimensional data sets per patient. Novel computational techniques allow the visualization of structures in these data and, finally, the identification of relevant subgroups. Correct data visualizations and projections from the high-dimensional space to the visualization plane require the correct representation of the structures in the data. This work shows that frequently used techniques are unreliable in this respect. One of the most important methods for data projection in this area is the t-distributed stochastic neighbor embedding (t-SNE). We analyzed its performance on artificial and real biomedical data sets. t-SNE introduced a cluster structure for homogeneously distributed data that did not contain any subgroup structure. In other data sets, t-SNE occasionally suggested the wrong number of subgroups or projected data points belonging to different subgroups, as if belonging to the same subgroup. As an alternative approach, emergent self-organizing maps (ESOM) were used in combination with U-matrix methods. This approach allowed the correct identification of homogeneous data while in sets containing distance or density-based subgroups structures; the number of subgroups and data point assignments were correctly displayed. The results highlight possible pitfalls in the use of a currently widely applied algorithmic technique for the detection of subgroups in high dimensional cytometric data and suggest a robust alternative.
Surge Margin Optimization of Centrifugal Compressors Using a New Objective Function Based on Local Flow Parameters
Authors: Ratz Johannes , Leichtfuß Sebastian , Beck Maximilian , Schiffer Heinz-Peter , Fröhlig Friedrich
Published: December 2019

Currently, 3D-CFD design optimization of centrifugal compressors in terms of the surge margin is one major unresolved issue. On that account, this paper introduces a new kind of objective function. The objective function is based on local flow parameters present at the design point of the centrifugal compressor. A centrifugal compressor with a vaned diffuser is considered to demonstrate the performance of this approach. By means of a variation of the beta angle distribution of the impeller and diffuser blade, 73 design variations are generated, and several local flow parameters are evaluated. Finally, the most promising flow parameter is transferred into an objective function, and an optimization is carried out. It is shown that the new approach delivers similar results as a comparable optimization with a classic objective function using two operating points for surge margin estimation, but with less computational effort since no second operating point near the surge needs to be considered.
Credit Scoring Using Machine Learning by Combing Social Network Information: Evidence from Peer-to-Peer Lending
Authors: Niu Beibei , Ren Jinzheng , Li Xiaotao
Published: December 2019

Financial institutions use credit scoring to evaluate potential loan default risks. However, insufficient credit information limits the peer-to-peer (P2P) lending platform’s capacity to build effective credit scoring. In recent years, many types of data are used for credit scoring to compensate for the lack of credit history data. Whether social network information can be used to strengthen financial institutions’ predictive power has received much attention in the industry and academia. The aim of this study is to test the reliability of social network information in predicting loan default. We extract borrowers’ social network information from mobile phones and then use logistic regression to test the relationship between social network information and loan default. Three machine learning algorithms—random forest, AdaBoost, and LightGBM—were constructed to demonstrate the predictive performance of social network information. The logistic regression results show that there is a statistically significant correlation between social network information and loan default. The machine learning algorithm results show that social network information can improve loan default prediction performance significantly. The experiment results suggest that social network information is valuable for credit scoring.
Updating the Reduct in Fuzzy β-Covering via Matrix Approaches While Adding and Deleting Some Objects of the Universe
Authors: Huang Jianxin , Yu Peiqiu , Li Weikang
Published: December 2019

Since fuzzy β -covering was proposed, few papers have focused on how to calculate the reduct in fuzzy β -covering and how to update the reduct while adding and deleting some objects of the universe. Here, we propose a matrix-based approach for computing the reduct in a fuzzy β -covering and updating it dynamically using a matrix. First, matrix forms for computing the reduct in a fuzzy β -covering are proposed. Second, properties of the matrix-based approaches are studied while adding and deleting objects. Then, matrix-based algorithms for updating the reduct in a fuzzy β -covering are proposed. Finally, the efficiency and validity of the designed algorithms are verified by experiments.
Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
Authors: Samerjai Chutharat , Sukontason Kabkaew L. , Sontigun Narin , Sukontason Kom , Klong-klaew Tunwadee , Chareonviriyaphap Theeraphap , Kurahashi Hiromu , Klimpel Sven , Kochmann Judith , Saeung Atiporn , Somboon Pradya , Wannasan Anchalee
Published: December 2019

Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMImin). Thus, the first crucial step is to correctly identify the fly species. Because of the high similarity among species of flesh flies, DNA-based identification is considered more favorable than morphology-based identification. In this study, we demonstrated the effectiveness of combined sequences (2216 to 2218 bp) of cytochrome c oxidase subunit I and II genes (COI and COII) for identification of the following 14 forensically important flesh fly species in Thailand: Boettcherisca nathani Lopes, Fengia ostindicae (Senior-White), Harpagophalla kempi (Senior-White), Liopygia ruficornis (Fabricius), Lioproctia pattoni (Senior-White), Lioproctia saprianovae (Pape & Bänziger), Parasarcophaga albiceps (Meigen), Parasarcophaga brevicornis (Ho), Parasarcophaga dux (Thomson), Parasarcophaga misera (Walker), Sarcorohdendorfia antilope (Böttcher), Sarcorohdendorfia inextricata (Walker), Sarcorohdendorfia seniorwhitei (Ho) and Seniorwhitea princeps (Wiedemann). Nucleotide variations of Thai flesh flies were evenly distributed throughout the COI-COII genes. Mean intra- and interspecific variations ranged from 0.00 to 0.96% and 5.22% to 12.31%, respectively. Using Best Match (BM) and Best Close Match (BCM) criteria, identification success for the combined genes was 100%, while the All Species Barcodes (ASB) criterion showed 76.74% success. Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses yielded similar tree topologies of monophyletic clades between species with very strong support values. The achieved sequences covering 14 forensically important flesh fly species including newly submitted sequences for B. nathani, F. ostindicae and S. seniorwhitei, can serve as a reliable reference database for further forensic entomological research in Thailand and in other areas where those species occur.
Comparative Transcriptome Analysis of Thitarodes Armoricanus in Response to the Entomopathogenic Fungi Paecilomyces Hepiali and Ophiocordyceps Sinensis
Authors: Rao Zhongchen , Cao Li , Wu Hua , Qiu Xuehong , Liu Guiqing , Han Richou
Published: December 2019

Thitarodes armoricanus is a medicinal and economically important Lepidopteran insect species. The larvae infected by Paecilomyces hepiali survive no more than four days, while those infected by Ophiocordyceps sinensis can survive for several months before mummification. This provides a valuable comparative system to study interactions between an insect host and different pathogenic fungi. By using the T. armoricanus genome, a time-course transcriptome analysis of the whole larvae without guts was performed to explore the larvae response to P. hepiali and O. sinensis infection. A total of 3106 differentially expressed genes in five clusters were identified. The genes involved in coagulation and multiple metabolisms were both suppressed after P. hepiali or O. sinensis infection, whereas those related to environmental information responses, cell processes, biotic stimulus, and immunity (such as cecropin (CEC)) were elevated. The rapid death of T. armoricanus after P. hepiali infection might be caused by osmotic imbalance, immunocompromise (such as DEFs and GLVs), and nervous system dysfunction (glutamatergic synapse). Up-regulation of the genes related to cuticle structure, nervous system (such as neurotrophin signal pathway and dopaminergic synapse) and immune effectors (such as attacin (ATT) and proline-rich antimicrobial peptide 1 (PRAMP1)) in T. armoricanus, may contribute to the co-existence of T. armoricanus and O. sinensis. This study provides a global view and potential key genes of the interaction between T. armoricanus and two fungal entomopathogens.
Taxonomic Revision of Neotropical Downeshelea Wirth and Grogan Predaceous Midges (Diptera: Ceratopogonidae)
Authors: Santarém Maria , Borkent Art , Felippe-Bauer Maria
Published: December 2019

The genus Downeshelea was described by Wirth and Grogan based on the diagnostic characters of the Monohelea multilineata species group. The first descriptions of species were based on body coloration, which resulted in confusion and misunderstanding of their identification. The aim of this study was to provide an updated diagnosis and description of Downeshelea, describe 18 new species, and redescribe 10 previously poorly described species. New records of species, a key for identification of all New World species, and a table with important morphometric data to distinguish both males and females of the various species are provided along with distribution maps of the 46 known New World species.
Factors Influencing Consumption of Edible Insects for Chinese Consumers
Authors: Liu Ai-Jun , Li Jie , Gómez Miguel I.
Published: December 2019

Edible insects are often considered a healthier and more sustainable meat substitute and protein source. Many studies have examined factors affecting the consumption behavior towards edible insects among Western consumers. However, little is known about factors influencing consumer behavior towards edible insects in Asian countries even though Asians have a long history of consuming insects. In this study, we surveyed 614 Chinese consumers from Beijing and Nanjing to examine the factors influencing their consumption and purchase behavior of edible insects. We find that insect phobia, feelings of disgust, knowledge level, and social demographic factors such as age, household size, household income and region (Northern or Southern China) are the main factors influencing purchase decisions. In addition, the results indicate that the perceived positive attributes associated with edible insects, the preferences of children in the household, as well as age and knowledge level have positive impacts on consumption frequency. On the other hand, concerns of food safety and the shape of the insects have negative impacts on consumption frequency. Finally, the results suggest that educating consumers to increase knowledge of edible insects increases their probability to purchase insect foods.
Electric Field Enhancing Artifacts as Precursors for Vacuum High-Voltage Breakdown
Authors: Behling Rolf
Published: December 2019

Abrupt formation of plasma in a high-voltage insulating vacuum gap and subsequent discharge of electrodes limits the reliability of a class of vacuum electronic devices, such as X-ray tubes. It has been suggested that electron field emission from negatively charged electrodes would precede and initiate such discharge. Heating and evaporation of material upon field emission would cause dense plasma to develop in periods of nanoseconds. High-pressure plasma would expand from the cathode, eventually bridging the gap. Nevertheless, the very reason for the unredictable initial development of discharge events after long periods of reliable operation is still matter of debate. Experience from industrial processes suggests hydrocarbon contamination to degrade the electric stability of high-voltage gaps. While former attempts aimed at explaining high field emission by carbonaceous 2D structures or surface resonance effects, this paper discusses whether 3D structures may grow slowly, until their evaporation in a matter of nanoseconds. Similar to the production of carbon nanotubes, protruding structures might comprise carbon and, in addition, metallic nanoparticles, which would boost production of vapor during their explosion. The hypothesis was tested by scanning electron and energy-dispersive X-ray inspection of two cathodes of medical X-ray tubes, covered with metallic seed nanoparticles, which served as model systems. A third cleaner cathode was inspected for comparison. Although certain suggested conditions of carbon feed, elevated substrate temperature and nanoparticle contamination of the surfaces were met, images showed only a very weak sign of growth of suspicious carbon structures. It seems, therefore, unlikely that CNT-like structures are a major cause of high-voltage breakdown between electrodes of X-ray tubes.
ZF-AutoML: An Easy Machine-Learning-Based Method to Detect Anomalies in Fluorescent-Labelled Zebrafish
Authors: Sawaki Ryota , Sato Daisuke , Nakayama Hiroko , Nakagawa Yuki , Shimada Yasuhito
Published: December 2019

Background: Zebrafish are efficient animal models for conducting whole organism drug testing and toxicological evaluation of chemicals. They are frequently used for high-throughput screening owing to their high fecundity. Peripheral experimental equipment and analytical software are required for zebrafish screening, which need to be further developed. Machine learning has emerged as a powerful tool for large-scale image analysis and has been applied in zebrafish research as well. However, its use by individual researchers is restricted due to the cost and the procedure of machine learning for specific research purposes. Methods: We developed a simple and easy method for zebrafish image analysis, particularly fluorescent labelled ones, using the free machine learning program Google AutoML. We performed machine learning using vascular- and macrophage-Enhanced Green Fluorescent Protein (EGFP) fishes under normal and abnormal conditions (treated with anti-angiogenesis drugs or by wounding the caudal fin). Then, we tested the system using a new set of zebrafish images. Results: While machine learning can detect abnormalities in the fish in both strains with more than 95% accuracy, the learning procedure needs image pre-processing for the images of the macrophage-EGFP fishes. In addition, we developed a batch uploading software, ZF-ImageR, for Windows (.exe) and MacOS (.app) to enable high-throughput analysis using AutoML. Conclusions: We established a protocol to utilize conventional machine learning platforms for analyzing zebrafish phenotypes, which enables fluorescence-based, phenotype-driven zebrafish screening.
Neopterin is Associated with Disease Severity and Outcome in Patients with Non-Ischaemic Heart Failure
Authors: Lanser Lukas , Pölzl Gerhard , Fuchs Dietmar , Weiss Günter , Kurz Katharina
Published: December 2019

Inflammation and immune activation play an important role in the pathogenesis of cardiac remodelling in patients with heart failure. The aim of this study was to assess whether biomarkers of inflammation and immune activation are linked to disease severity and the prognosis of heart failure patients. In 149 patients (65.8% men, median age 49.7 years) with heart failure from nonischaemic cardiomyopathy, the biomarkers neopterin and C-reactive protein were tested at the time of diagnosis. Patients were followed-up for a median of 58 months. During follow-up, nineteen patients died, five had a heart transplantation, two needed a ventricular assistance device, and twenty-one patients had to be hospitalised because of heart failure decompensation. Neopterin concentrations correlated with N-terminal prohormone of brain natriuretic peptide (NT-proBNP) concentrations (rs = 0.399, p < 0.001) and rose with higher New York Heart Association (NYHA) class (I: 5.60 nmol/L, II: 6.90 nmol/L, III/IV: 7.80 nmol/L, p = 0.033). Higher neopterin levels were predictive for an adverse outcome (death or hospitalisation due to HF decompensation), independently of age and sex and of established predictors in heart failure such as NYHA class, NT-proBNP, estimated glomerular filtration rate (eGFR), and left ventricular ejection fraction (LV-EF) (HR 2.770; 95% CI 1.419–5.407; p = 0.003). Patients with a neopterin/eGFR ratio ≥ 0.133 (as a combined marker for immune activation and kidney function) had a more than eightfold increased risk of reaching an endpoint compared to patients with a neopterin/eGFR ratio ≤0.065 (HR 8.380; 95% CI 2.889–24.308; p < 0.001). Neopterin is associated with disease severity and is an independent predictor of prognosis in patients with heart failure.
Composite Reinforcement Architectures: A Review of Field-Assisted Additive Manufacturing for Polymers
Authors: Roy Madhuparna , Tran Phong , Dickens Tarik , Schrand Amanda
Published: December 2019

The demand for additively manufactured polymer composites with increased specific properties and functional microstructure has drastically increased over the past decade. The ability to manufacture complex designs that can maximize strength while reducing weight in an automated fashion has made 3D-printed composites a popular research target in the field of engineering. However, a significant amount of understanding and basic research is still necessary to decode the fundamental process mechanisms of combining enhanced functionality and additively manufactured composites. In this review, external field-assisted additive manufacturing techniques for polymer composites are discussed with respect to (1) self-assembly into complex microstructures, (2) control of fiber orientation for improved interlayer mechanical properties, and (3) incorporation of multi-functionalities such as electrical conductivity, self-healing, sensing, and other functional capabilities. A comparison between reinforcement shapes and the type of external field used to achieve mechanical property improvements in printed composites is addressed. Research has shown the use of such materials in the production of parts exhibiting high strength-to-weight ratio for use in aerospace and automotive fields, sensors for monitoring stress and conducting electricity, and the production of flexible batteries.
Physicochemical, Mechanical, and Antimicrobial Properties of Novel Dental Polymers Containing Quaternary Ammonium and Trimethoxysilyl Functionalities
Authors: Bienek Diane R. , Giuseppetti Anthony A. , Frukhtbeyn Stanislav A. , Hiers Rochelle D. , Esteban Florez Fernando L. , Khajotia Sharukh S. , Skrtic Drago
Published: December 2019

The aims of this study were to evaluate the physicochemical and mechanical properties, antimicrobial (AM) functionality, and cytotoxic potential of novel dental polymers containing quaternary ammonium and trimethoxysilyl functionalities (e.g., N-(2-(methacryloyloxy)ethyl)-N,N-dimethyl-3-(trimethoxysilyl)propan-1-aminium iodide (AMsil1) and N-(2-(methacryloyloxy)ethyl)-N,N-dimethyl-11-(trimethoxysilyl)undecan-1-aminium bromide (AMsil2)). AMsil1 or AMsil2 were incorporated into light-cured (camphorquinone + ethyl-4-N,N-dimethylamino benzoate) urethane dimethacrylate (UDMA)/polyethylene glycol-extended UDMA/ethyl 2-(hydroxymethyl)acrylate (EHMA) resins (hereafter, UPE resin) at 10 or 20 mass %. Cytotoxic potential was assessed by measuring viability and metabolic activity of immortalized mouse connective tissue and human gingival fibroblasts in direct contact with monomers. AMsil–UPE resins were evaluated for wettability by contact angle measurements and degree of vinyl conversion (DVC) by near infra-red spectroscopy analyses. Mechanical property evaluations entailed flexural strength (FS) and elastic modulus (E) testing of copolymer specimens. The AM properties were assessed using Streptococcus mutans (planktonic and biofilm forms) and Porphyromonas gingivalis biofilm. Neither AMsil exhibited significant toxicity in direct contact with cells at biologically relevant concentrations. Addition of AMsils made the UPE resin more hydrophilic. DVC values for the AMsil–UPE copolymers were 2–31% lower than that attained in the UPE resin control. The mechanical properties (FS and E) of AMsil–UPE specimens were reduced (11–57%) compared to the control. Compared to UPE resin, AMsil1–UPE and AMsil2–UPE (10% mass) copolymers reduced S. mutans biofilm 4.7- and 1.7-fold, respectively (p ≤ 0.005). Although not statistically different, P. gingivalis biofilm biomass on AMsil1–UPE and AM AMsil2–UPE copolymer disks were lower (71% and 85%, respectively) than that observed with a commercial AM dental material. In conclusion, the AM function of new monomers is not inundated by their toxicity towards cells. Despite the reduction in mechanical properties of the AMsil–UPE copolymers, AMsil2 is a good candidate for incorporation into multifunctional composites due to the favorable overall hydrophilicity of the resins and the satisfactory DVC values attained upon light polymerization of AMsil-containing UDMA/PEG-U/EHMA copolymers.
A Study on the Air Cavity under a Stepped Planing Hull
Authors: Yang Dongmei , Sun Zhiyuan , Jiang Yi , Gao Zeyang
Published: December 2019

Based on the FVM (finite volume method) numerical method, the flow field around the stepped planing hull in Taunton series was simulated. According to the general procedure of numerical uncertainty analysis, the numerical uncertainty in the high-speed flow field simulation of the stepped planing hull was discussed. Combined with the wave-making characteristics of the hull, the generation mechanism, shape evolution of air cavity, and the pressure distribution characteristics under the influence of the cavity, focuses on the variation of the flow around the stepped planing when the hull is in the triangle planing stage. Numerical results suggest that, as the air cavity enlarges, the cover rate of the air cavity can rise up to 77.8% of the whole wetted surface of the planing hull bottom. While, in the triangle planing stage, there is additional wetting at the aft bilge, which leads to the decrease of the air cavity rate and the increase of the wetted area. At the same time, the pressure distribution concentrates to the center of gravity.
Influence of Mild Bottom Slopes on the Overtopping Flow over Mound Breakwaters under Depth-Limited Breaking Wave Conditions
Authors: Mares-Nasarre Patricia , Gómez-Martín M. Esther , Medina Josep R.
Published: December 2019

The crest elevation of mound breakwaters is usually designed considering a tolerable mean wave overtopping discharge. However, pedestrian safety, characterized by the overtopping layer thickness (OLT) and the overtopping flow velocity (OFV), is becoming more relevant due to the reduction of the crest freeboards of coastal structures. Studies in the literature focusing on OLT and OFV do not consider the bottom slope effect, even if it has a remarkable impact on mound breakwater design under depth-limited breaking wave conditions. Therefore, this research focuses on the influence of the bottom slope on OLT and OFV exceeded by 2% of incoming waves, hc,2% and uc,2%. A total of 235 2D physical tests were conducted on conventional mound breakwaters with a single-layer Cubipod® and double-layer rock and cube armors with 2% and 4% bottom slopes. Neural networks were used to determine the optimum point to estimate wave characteristics for hc,2% and uc,2% calculation; that point was located at a distance from the model toe of three times the water depth at the toe (hs) of the structure. The influence of the bottom slope is studied using trained neural networks with fixed wave conditions in the wave generation zone; hc,2% slightly decreases and uc,2% increases as the gradient of the bottom slope increases.