bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Feeding Behavior and Life Table of Nilaparvata lugens and Sogatella furcifera (Hemiptera: Delphacidae) under Sublethal Concentrations of Imidacloprid and Sulfoxaflor
Authors: Kang Yeolgyu , Koo Hyun-Na , Kim Hyun-Kyung , Kim Gil-Hah
Published: December 2022

The brown planthopper (BPH) Nilaparvata lugens and white-backed planthopper (WBPH) Sogatella furcifera are serious rice insect pests that cannot overwinter in Korea and migrate from southeast Asian countries and China. In this study, we investigated the sublethal effects of imidacloprid and sulfoxaflor on the biological parameters and feeding behavior of planthoppers. These sublethal concentrations significantly decreased the net reproduction rate (R0), the intrinsic rate of increase (rm), and the mean generation time (T). For BPHs, the total durations of nonpenetration (NP) waveform by imidacloprid (LC10 = 164.74 and LC30 = 176.48 min) and sulfoxaflor (LC10 = 235.57 and LC30 = 226.93 min) were significantly different from those in the control group (52.73 min). In addition, on WBPHs, the total durations of NP waveform by imidacloprid (LC10 = 203.69 and LC30 = 169.9 min) and sulfoxaflor (LC10 = 134.02 and LC30 = 252.14 min) were significantly different from those in the control group (45.18 min). Moreover, the LC10 and LC30 of these insecticides significantly decreased the phloem feeding time. In conclusion, imidacloprid had a better effect on the inhibition of feeding of the WBPH, and sulfoxaflor showed a better effect on the inhibition of feeding of the BPH. Therefore, it is suggested that treatment with sublethal concentrations of the above insecticides will reduce the feeding of BPHs and WBPHs on rice phloem.
Domestic Triatoma spp. Infections with Trypanosoma cruzi, Household Infestations, and Molecular Identification in Oaxaca, México
Authors: Fernández-Santos Nadia A. , Trujillo-García Josefina C. , Hamer Sarah A. , Wei Lihua , Martínez-Montoya Humberto , Tamez-Guerra Patricia , Hamer Gabriel L. , Rodríguez-Pérez Mario A.
Published: December 2022

In Latin America, Mexico is the country with the second highest annual estimated number of Chagas disease cases, caused by Trypanosoma cruzi, due to vector-borne transmission. The state of Oaxaca is the location of the first documented human cases of Chagas disease in Mexico and contained the highest T. cruzi seropositive rate (3.5%) from blood donors. Here, entomological surveys, from 2017 to 2019, were conducted to collect triatomines in 124 villages of 60 municipalities. Four principal domestic Triatoma spp. (Hemiptera: Triatominae), Triatoma phyllosoma, T. barberi, T. mazzotti, and T. dimidiata, of Oaxaca, Mexico were identified by morphology and molecular analysis of the barcode region of the cytochrome oxidase 1 (cox1 or COI or CO1) gene. A total of 41 out of 83 T. phyllosoma specimens examined by microscopy were positive for T. cruzi (49%), 49 out of 171 for T. barberi (28%), 31 out of 177 for T. mazzotti (17%), and none out of 10 for T. dimidiata (0%). Overall, the infestation index was 3.1% of households containing at least one triatomine; the crowding index was a mean of two Triatoma spp./household; and the colonization index was 0.38 for households based on presence of nymphs. Geographical distribution of triatomines in Oaxaca at the municipality level and endophilic behavior is also reported. Precise identification, endophilic habits, and infection rates of these triatomines are paramount for vector control programs of the Ministry of Health of Oaxaca and beyond.
Single Night Surveys of Moth Communities Can Serve as Ultra-Rapid Biodiversity Assessments
Authors: Duran Daniel P. , Timar Matthew , Rothauser Blaine
Published: December 2022

Biodiversity conservation decisions are typically based on limited data and resources. For this reason, there is great interest in surveying taxa that may allow for a rapid assessment of the biodiversity at a site. Numerous taxa have been proposed and utilized for rapid assessments that allow for such a survey in a matter of weeks or less. Herein, we test the idea that nocturnal moths have many of the characteristics that make them ideal for such surveys, such as relative ease of identification, strong ecological association with specific plant species and habitats, high alpha diversity, extended seasonal activity, and ease of trapping. We demonstrate that even in a few hours of sampling during single night surveys, moth communities are predictive of regional forest types at sampling sites in New Jersey. We sampled moths in five different forest habitats in New Jersey, USA: Pine Barrens, Upland Deciduous Forest, Palustrine Deciduous Forest, Maritime Forest, and Ruderal/Disturbed Forests, at four sites per forest type. Non-metric multidimensional scaling (NMDS) analyses revealed that moth communities differ significantly across these four forest types (p < 0.01). We used Analysis of Similarity (ANOSIM) R tests to quantify the degree of differentiation among moth communities, and found that Tortricidae (R = 0.657) and Geometridae (R = 0.637) predict forest communities nearly as well as the total moth diversity (R = 0.668). Uncommon species (R = 0.665) were better predictors than common species (R = 0.500). Host plant generalists (R = 0.654) were better predictors than specialists (0.538), which was a surprising find.
Kupffer Cells as a Target for Immunotherapy
Authors: Sakai Takashi , Lin Wen-Ling
Published: December 2022

Kupffer cells (KCs) are resident macrophages in the liver. Recent studies have revealed that KCs are closely related to inflammatory liver diseases, including nonalcoholic liver diseases (NAFLD). From this point of view, KC transplantation can be a candidate for immunotherapy against inflammatory diseases. Similar to general macrophages, KCs show several different phenotypes according to their environment. Activated KCs are involved in either proinflammatory responses or anti-inflammatory responses. Thus, to manipulate KCs for immunotherapy, it is crucial to control the direction of KC activation. Here, we summarize the outlook and the issues hindering immunotherapy using KC transplantation.
Intravascular Ultrasound Guidance Is Associated with a Favorable One-Year Target Vessel Failure Rate and No Residual Myocardial Ischemia after the Percutaneous Treatment of Very Long Coronary Artery Lesions
Authors: Budrys Povilas , Baranauskas Arvydas , Davidavicius Giedrius
Published: December 2022

Background: Studies have shown that percutaneous coronary intervention (PCI) in long coronary artery lesions (≥30 mm) is associated with more frequent target vessel failure (TVF), and a significant proportion of patients have lesions that continue to induce ischemia after PCI (FFR ≤ 0.8). We investigated the impact of intravascular ultrasound (IVUS) on the functional PCI result and one-year TVF rate after the percutaneous treatment of long coronary artery lesions. Methods: A total of 80 patients underwent IVUS-guided PCI in long coronary artery lesions. The PCI results were validated with IVUS and FFR. Procedural outcomes were the proportion of patients with: (1) optimal physiology result (post PCI FFR value ≥ 0.9); (2) optimal anatomy result (all IVUS PCI optimization criteria met); and (3) optimal physiology and anatomy result. The clinical outcome was TVF during a one-year follow-up (target vessel (TV)-related death, TV myocardial infarction, ischemia-driven TV revascularization). Results: The mean stented segment length was 62 mm. The target vessel (TV) was the left anterior descending artery in 82.5% of cases. There were no patients with residual ischemia (FFR ≤ 0.8) after PCI. Optimal coronary flow (FFR ≥ 0.9) was achieved in 37.5%; optimal anatomy, as assessed by IVUS, was achieved in 68.4%; and both optimal flow and anatomy were achieved in 25% of patients. Target vessel failure during the 12-month follow-up was 2.5%. Conclusions: In the percutaneous treatment of very long coronary artery lesions, the use of IVUS guidance is associated with a low TVF rate during a one-year follow-up and no residual myocardial ischemia, as assessed by FFR.
Intravenous Thrombolysis for Acute Ischemic Stroke in Patients with End-Stage Renal Disease on Hemodialysis: A Narrative Review
Authors: Egashira Shuhei , Koga Masatoshi , Toyoda Kazunori
Published: December 2022

Objectives: Acute ischemic stroke (AIS) is a significant and devastating complication in patients with end-stage renal disease on hemodialysis (ESRD/HD). Since one-third of AIS in ESRD/HD patients occurs during or soon after dialysis, patients are more likely to present within the time window when intravenous thrombolysis (IVT) can be performed. IVT may improve prognosis in ESRD/HD patients with AIS. However, ESRD/HD patients have been excluded from large trials and may have been withheld from IVT due to concerns about bleeding complications. To date, there is no clear evidence and firm guidance on the safety and efficacy of IVT in ESRD/HD patients with AIS. This narrative review aimed to evaluate critical scientific data on the benefits and risks of IVT use in patients with ESRD/HD and AIS. Materials and Methods: We searched the electronic database of PubMed for studies evaluating the relationship between AIS, ESRD/HD, and IVT. Reference sections and additional publications were also searched manually. Studies on AIS in patients with ESRD/HD requiring maintenance dialysis that referred to IVT were included. Results: In total, 560 studies were found in the PubMed electronic database during the period covered, of which 10 met the selection criteria. IVT for AIS in ESRD/HD patients could improve neurological outcomes and be safely performed even with the possibility of hemorrhagic complications associated with hypertension. Despite the high complication and mortality rates in ESRD/HD patients with AIS after IVT, the association with IVT was unclear. Conclusions: IVT for AIS in ESRD/HD patients may improve outcomes and should not be withheld based solely on ESRD/HD status.
Comparison between Cystatin C- and Creatinine-Based Estimated Glomerular Filtration Rate in the Follow-Up of Patients Recovering from a Stage-3 AKI in ICU
Authors: Nateghi Haredasht Fateme , Viaene Liesbeth , Vens Celine , Callewaert Nico , De Corte Wouter , Pottel Hans
Published: December 2022

Background: Acute kidney injury (AKI) in critically ill patients is associated with a significant increase in mortality as well as long-term renal dysfunction and chronic kidney disease (CKD). Serum creatinine (SCr), the most widely used biomarker to evaluate kidney function, does not always accurately predict the glomerular filtration rate (GFR), since it is affected by some non-GFR determinants such as muscle mass and recent meat ingestion. Researchers and clinicians have gained interest in cystatin C (CysC), another biomarker of kidney function. The study objective was to compare GFR estimation using SCr and CysC in detecting CKD over a 1-year follow-up after an AKI stage-3 event in the ICU, as well as to analyze the association between eGFR (using SCr and CysC) and mortality after the AKI event. Method: This prospective observational study used the medical records of ICU patients diagnosed with AKI stage 3. SCr and CysC were measured twice during the ICU stay and four times following diagnosis of AKI. The eGFR was calculated using the EKFC equation for SCr and FAS equation for CysC in order to check the prevalence of CKD (defined as eGFR < 60 mL/min/1.73 m2). Results: The study enrolled 101 patients, 36.6% of whom were female, with a median age of 74 years (30–92), and a median length of stay of 14.5 days in intensive care. A significant difference was observed in the estimation of GFR when comparing formulas based on SCrand CysC, resulting in large differences in the prediction of CKD. Three months after the AKI event, eGFRCysC < 25 mL/min/1.73 m2 was a predictive factor of mortality later on; however, this was not the case for eGFRSCr. Conclusion: The incidence of CKD was highly discrepant with eGFRCysC versus eGFRSCr during the follow-up period. CysC detects more CKD events compared to SCr in the follow-up phase and eGFRCysC is a predictor for mortality in follow-up but not eGFRSCr. Determining the proper marker to estimate GFR in the post-ICU period in AKI stage-3 populations needs further study to improve risk stratification.
Evaluation of the New SingularityTM Air versus Ambu® Aura GainTM: A Randomized, Crossover Mannequin Study
Authors: Gasteiger Lukas , Hornung Rouven , Woyke Simon , Hoerner Elisabeth , Neururer Sabrina , Moser Berthold
Published: December 2022

Background: This randomised crossover mannequin study aimed to compare the insertion time for the newly developed SingularityTM Air and the Ambu® AuraGainTM. The SingularityTM Air includes a bendable tube in order to allow optimal passform. Methods: Fifty anaesthetists with a minimum of 100 supraglottic airway device insertions were recruited and randomly assigned to start either with the SingularityTM Air or with the Ambu® AuraGainTM. Participants watched a tutorial video the day before the assessment and received a standardized introduction immediately before the assessment. The primary outcome was the time for successful insertion. Secondary parameters were the overall insertion success rate, the numbers of insertion attempts (maximum three), the glottic view through a flexible bronchoscope, and the success rate for gastric tube insertion. Results: Fifty participants were eventually recruited and randomly assigned to insert both devices according to the randomization. The insertion time was 24 s for SingularityTM Air as compared to 20 s for Ambu® AuraGainTM (p < 0.001). Overall insertion rate was 92% for the SingularityTM Air as compared to 100% for the Ambu® AuraGainTM (p could not be derived as one variable is a constant). The primary insertion success rate was better for the Ambu® AuraGainTM than for the SingularityTM Air (94% versus 68%; p: 0.002, respectively). Conclusion: The time for successful insertion and the insertion success rate for the newly developed SingularityTM Air is inferior to that for the Ambu® AuraGainTM.
Goals of Care, Critical Care Utilization and Clinical Outcomes in Obese Patients Admitted under General Medicine
Authors: Lim Andy K. H. , Rathnasekara Greasha K. , Kanumuri Priyanka , Malawaraarachchi Janith K. , Song Zheng , Curtis Claire A.
Published: December 2022

Obesity is associated with long-term morbidity and mortality, but it is unclear if obesity affects goals of care determination and intensive care unit (ICU) resource utilization during hospitalization under a general medicine service. In a cohort of 5113 adult patients admitted under general medicine, 15.3% were obese. Patients with obesity were younger and had a different comorbidity profile than patients who were not obese. In age-adjusted regression analysis, the distribution of goals of care categories for patients with obesity was not different to patients who were not obese (odds ratio for a lower category with more limitations, 0.94; 95% confidence interval [CI]: 0.79–1.12). Patients with obesity were more likely to be directly admitted to ICU from the Emergency Department, require more ICU admissions, and stayed longer in ICU once admitted. Hypercapnic respiratory failure and heart failure were more common in patients with obesity, but they were less likely to receive mechanical ventilation in favor of non-invasive ventilation. The COVID-19 pandemic was associated with 16% higher odds of receiving a lower goals of care category, which was independent of obesity. Overall hospital length of stay was not affected by obesity. Patients with obesity had a crude mortality of 3.8 per 1000 bed-days, and age-adjusted mortality rate ratio of 0.75 (95% CI: 0.49–1.14) compared to patients who were not obese. In conclusion, there was no evidence to suggest biased goals of care determination in patients with obesity despite greater ICU resource utilization.
Clinical and Radiological Outcomes after Total Shoulder Arthroplasty Using Custom-Made Glenoid Components: A Systematic Review
Authors: Gruber Michael Stephan , Schwarz Tamara , Lindorfer Marlene , Rittenschober Felix , Bischofreiter Martin , Hochreiter Josef , Ortmaier Reinhold
Published: December 2022

Reverse total shoulder arthroplasty presents itself sometimes as challenging when it comes to addressing massive bone loss, either in primary or revision settings. Custom components recently have made their way into shoulder prosthetics and are meant to help in the case of extensive glenoid bone destruction. Because of strict indication and the fairly recent introduction of these implants, the usage of custom-made glenoid implants is not very common yet. However, the early results are promising. The purpose of this review was to summarize and analyze the available literature. Therefore, a systematic review was performed according to PRISMA guidelines. A comprehensive search of the databases PubMed, Cochrane, and Livivo was performed to screen for studies reporting on clinical and radiological outcomes of custom glenoid implants. Four studies with a total of 46 shoulders were included in this review. The mean patient age was 68.8 years and the mean time of follow-up was 24.3 months. The weighted means showed an increase in CMS (32.7 points), in ASES (39.8 points), in anteversion (67.4 degrees), and in abduction (51.9 degrees) and a decrease in VAS (5.4 points). Custom-made glenoid implants are therefore a viable option in cases of large combined glenoid bone loss, both in primary and revision shoulder arthroplasty.
Management and Predictors of Treatment Failure in Patients with Chemo-Resistant/Relapsed Gestational Trophoblastic Neoplasia with Lung Metastasis
Authors: Kong Yujia , Wang Weidi , Lin Jinkai , Wan Xirun , Feng Fengzhi , Ren Tong , Zhao Jun , Yang Junjun , Xiang Yang
Published: December 2022

The aim of the study was to assess the effectiveness of a combined treatment modality of salvage chemotherapy and pulmonary resection in chemo-resistant/relapsed gestational trophoblastic neoplasia (GTN) with lung metastasis and identify predictors of treatment failure. Data of patients with chemo-resistant/relapsed GTN with lung metastasis who received salvage chemotherapy combined with pulmonary resection were retrospectively analyzed. Among 134 included patients, the number of preoperative chemotherapy regimens ranged from 2–8 (median, 3), and courses ranged from 4–37 (median, 14). Pulmonary lobectomies, segmentectomies, wedge resections, and lobectomies plus wedge resections were performed in 84, 5, 35, and 10 patients, respectively. After completion of treatment, 130 (97.0%) patients achieved complete remission. In the entire cohort, the 5-year overall survival (OS) rate was 87.6%. OS rates were similar between stage III and stage IV disease cohorts (89.4% vs. 75.0%, p = 0.137). Preoperative β-human chorionic gonadotropin (β-hCG) levels > 10 IU/L (p = 0.027) and number of preoperative chemotherapy regimens > 3 (p = 0.018) were predictors of treatment failure. The combined treatment modality of salvage chemotherapy and pulmonary resection is effective in patients with chemo-resistant/relapsed GTN with lung metastasis, improving their prognoses. Patients with preoperative serum β-hCG >10 IU/L and those with >3 chemotherapy regimens preoperatively may not benefit from this multidisciplinary treatment.
Effects of Fluids on the Sublingual Microcirculation in Sepsis
Authors: Cusack Rachael , O’Neill Susan , Martin-Loeches Ignacio
Published: December 2022

Sepsis is one of the most common and deadly syndromes faced in Intensive Care settings globally. Recent advances in bedside imaging have defined the changes in the microcirculation in sepsis. One of the most advocated interventions for sepsis is fluid therapy. Whether or not fluid bolus affects the microcirculation in sepsis has not been fully addressed in the literature. This systematic review of the evidence aims to collate studies examining the microcirculatory outcomes after a fluid bolus in patients with sepsis. We will assimilate the evidence for using handheld intra vital microscopes to guide fluid resuscitation and the effect of fluid bolus on the sublingual microcirculation in patients with sepsis and septic shock. We conducted a systematic search of Embase, CENTRAL and Medline (PubMed) using combinations of the terms “microcirculation” AND “fluid” OR “fluid resuscitation” OR “fluid bolus” AND “sepsis” OR “septic shock”. We found 3376 potentially relevant studies. Fifteen studies published between 2007 and 2021 fulfilled eligibility criteria to be included in analysis. The total number of participants was 813; we included six randomized controlled trials and nine non-randomized, prospective observational studies. Ninety percent used Sidestream Dark Field microscopy to examine the microcirculation and 50% used Hydroxyethyl Starch as their resuscitation fluid. There were no clear effects of fluid on the microcirculation parameters. There was too much heterogeneity between studies and methodology to perform meta-analysis. Studies identified heterogeneity of affect in the sepsis population, which could mean that current clinical classifications were not able to identify different microcirculation characteristics. Use of microcirculation as a clinical endpoint in sepsis could help to define sepsis phenotypes. More research into the effects of different resuscitation fluids on the microcirculation is needed.
Primary Functioning Hepatic Paraganglioma Treated by Laparoscopy: A Case Report
Authors: Jiang Chenhao , Chen Chuwen , Zhou Yongjie , Yang Jian , Yang Jiayin
Published: December 2022

Paragangliomas are highly vascularised and often heritable tumors derived from the paraganglia. They are typically discovered in the retroperitoneal space as well as the head and neck region but are rarely encountered in the liver parenchyma. We report a case of a primary functioning hepatic paraganglioma and provide an up-to-date literature review of patients with such tumors. We present a case of functioning paraganglioma in a 34-year-old female patient who suffered a solitary lesion in her left lateral lobe with symptoms of hypertension since pregnancy. She did not have any family history and her pre-pregnancy examination was negative. An abdominal CT imaging revealed a 6.5 × 5.7 cm liver lesion in segments II and III. Laboratory investigations identified elevation in plasma-free catecholamines. With sufficient preoperative preparation, the patient underwent laparoscopic left hemihepatectomy. Immunohistochemical staining revealed Syn (+) tumor cell nests surrounded by S-100 sustentacular cells (+), providing a definitive diagnosis of paraganglioma. The patient recovered uneventfully without signs of recurrence during a 1-year follow-up period. Our case demonstrates that primary refractory hypertension in pregnancy should be screened for paraganglioma through abdominal ultrasound and plasma free catecholamines. On the other hand, laparoscopic surgery is technically safe and feasible for the treatment of patients with hepatic paragangliomas in favorable locations.
Investigation of Cell Adhesion and Cell Viability of the Endothelial and Fibroblast Cells on Electrospun PCL, PLGA and Coaxial Scaffolds for Production of Tissue Engineered Blood Vessel
Authors: Bazgir Morteza , Saeinasab Morvarid , Zhang Wei , Zhang Ximu , Min Tsui Ka , Maasoumi Sarvestani Abolfazl , Nawaz Subhaan , Coates Phil , Youseffi Mansour , Elies Jacobo , Sefat Farshid
Published: December 2022

Endothelialization of artificial scaffolds is considered an effective strategy for increasing the efficiency of vascular transplantation. This study aimed to compare the biophysical/biocompatible properties of three different biodegradable fibrous scaffolds: Poly (ɛ-caprolactone) (PCL) alone, Poly Lactic-co-Glycolic Acid (PLGA) alone (both processed using Spraybase® electrospinning machine), and Coaxial scaffold where the fiber core and sheath was made of PCL and PLGA, respectively. Scaffold structural morphology was assessed by scanning electron microscope and tensile testing was used to investigate the scaffold tension resistance over time. Biocompatibility studies were carried out with human umbilical vein endothelial cells (HUVEC) and human vascular fibroblasts (HVF) for which cell viability (and cell proliferation over a 4-day period) and cell adhesion to the scaffolds were assessed by cytotoxicity assays and confocal microscopy, respectively. Our results showed that all biodegradable polymeric scaffolds are a reliable host to adhere and promote proliferation in HUVEC and HVF cells. In particular, PLGA membranes performed much better adhesion and enhanced cell proliferation compared to control in the absence of polymers. In addition, we demonstrate here that these biodegradable membranes present improved mechanical properties to construct potential tissue-engineered vascular graft.
Application of Tribology Concept in Dental Composites Field: A Scoping Review
Authors: La Rosa Giusy Rita Maria , Generali Luigi , Bugea Calogero , Ounsi Hani F. , Benyőcs Gergely , Neelakantan Prasanna , Pedullà Eugenio
Published: December 2022

Tribology is the discipline concerning the application of friction, lubrication, and wear concepts of interacting surfaces in relative motion. A growing interest has developed in tribology application in medical biomaterials, such as resin composites used in restorative dentistry. Yet, the keywords “tribology” and “biotribology” are little applied in the pertinent publications. The aim of this scoping review was to offer an overview of tribology application in dental composites research and to identify knowledge gaps and address future research. A literature search was conducted on Pubmed and Scopus databases and the studies investigating the tribological behavior of resin composites were included for qualitative synthesis. The majority of studies on dental tribology were published in the research areas of mechanical engineering/nanotechnology and differed in several methodological aspects. The preponderant engineering approach and the lack of standardized testing make the laboratory findings poorly informative for clinicians. Future research should focus on the tribological behavior of dental materials composites by means of an integrated approach, i.e., engineering and clinical, for improving development and advancement in this field of research.
Adhesive Property of 3D-Printed PEEK Abutments: Effects of Surface Treatment and Temporary Crown Material on Shear Bond Strength
Authors: Wang Dingjie , Han Xingting , Luo Feng , Thieringer Florian M. , Xu Yichen , Ou Guomin , Spintzyk Sebastian
Published: December 2022

Three-dimensionally printed polyetheretherketone (PEEK) materials are promising for fabricating customized dental abutments. This study aimed to investigate the adhesive property of a 3D-printed PEEK material. The effects of surface treatment and temporary crown materials on shear bond strength were evaluated. A total of 108 PEEK discs were 3D printed by fused-filament fabrication. Surface treatments, including sandblasting, abrasive paper grinding, and CO2 laser ablation, were applied to the PEEK discs, with the untreated specimens set as the control. Afterward, the surface topographies of each group were investigated by scanning electron microscopy (SEM, n = 1) and roughness measurements (n = 7). After preparing the bonding specimens with three temporary crown materials (Artificial teeth resin (ATR), 3M™ Filtek™ Supreme Flowable Restorative (FR), and Cool Temp NATURAL (CTN)), the shear bond strength was measured (n = 6), and the failure modes were analyzed by microscopy and SEM. The results showed that ATR exhibited a significantly higher shear bond strength compared to FR and CTN (p < 0.01), and the PEEK surfaces treated by sandblasting and abrasive paper grinding showed a statistically higher shear bond strength compared to the control (p < 0.05). For clinical application, the ATR material and subtractive surface treatments are recommended for 3D-printed PEEK abutments.
All-Standard-Cell-Based Analog-to-Digital Architectures Well-Suited for Internet of Things Applications
Authors: Correia Ana , Tavares Vítor Grade , Barquinha Pedro , Goes João
Published: December 2022

In this paper, the most suited analog-to-digital (A/D) converters (ADCs) for Internet of Things (IoT) applications are compared in terms of complexity, dynamic performance, and energy efficiency. Among them, an innovative hybrid topology, a digital–delta (Δ) modulator (ΔM) ADC employing noise shaping (NS), is proposed. To implement the active building blocks, several standard-cell-based synthesizable comparators and amplifiers are examined and compared in terms of their key performance parameters. The simulation results of a fully synthesizable Digital-ΔM with NS using passive and standard-cell-based circuitry show a peak of 72.5 dB in the signal-to-noise and distortion ratio (SNDR) for a 113 kHz input signal and 1 MHz bandwidth (BW). The estimated FoMWalden is close to 16.2 fJ/conv.-step.
Design and Optimization of Multipoint Sampler for Seafloor Sediment Carried by a Deep-Sea Landing Vehicle
Authors: Gao Yan , Zhou Yue , Guo Wei , Fu Yifan , Gao Sen , Wei Zhenzhuo , Sun Hongming , Sun Yu
Published: December 2022

The present study proposes a low-energy consumption multipoint sampler carried by a deep-sea landing vehicle (DSLV) to meet the requirements of time series sampling in local areas and location series sampling in wide areas, and an optimization method of sampling structure based on least-squares support-vector machine (LSSVM) surrogate model and a multi-objective particle swarm optimization (MOPSO) algorithm. First, the overall structure and core components, such as the multipoint sampler’s sampling structure, were designed. The optimization variables were the cone angle, sampling tube inner diameter, and sampling tube inner hole length, which were determined by considering the force with which the sampling structure penetrates the seafloor sediment. Then, the sampling process was simulated by the finite element method-smoothed particle hydrodynamics (FEM-SPH) method, while the accurate LSSVM model of force required for sampling and sampling tube volume was established. Finally, the MOPSO algorithm was used for multi-objective optimization of model parameters of sampling structure. The optimal model of sampling structure that can provide theoretical support for the optimal design of multipoint sampler effectively reduces energy consumption and improves sampling efficiency by force required for sampling 25.89% lower and sampling tube volume 34.81% higher than the original model.
Mate Perception and Gene Networks Regulating the Early Phase of Sex in Pseudo-nitzschia multistriata
Authors: Marotta Pina , Borgonuovo Camilla , Santin Anna , Russo Monia Teresa , Manfellotto Francesco , Montresor Marina , De Luca Pasquale , Ferrante Maria Immacolata
Published: December 2022

Diatoms are photosynthetic microorganisms playing a key role in the functioning of aquatic ecosystems; they are at the base of the food web and are the main drivers of biogeochemical processes. These microalgae have a unique diplontic life cycle in which the vegetative phase entails a cell size reduction that would lead to the extinction of the cell population if the size was not restored, usually by sexual reproduction. The switch from asexual to sexual reproduction needs to be finely synchronized and regulated to ensure its success; to this aim, cells evolved complex chemical crosstalk that mediates mating. We focused our attention on the marine diatom Pseudo-nitzschia multistriata, investigating the reciprocal perception of the opposite mating type (MT) and the genes and signaling molecules putatively involved in the process. From previously available transcriptomic data, we selected a panel of genes deregulated during the early phase of sexual reproduction, confirming for some of them a role during mate perception and establishing a hierarchy governing their behavior. Moreover, we explored the nature of the molecules controlling sexual reproduction in this species, unveiling that the signaling is mediated by the secretion of protein and non-protein cues from the MT− and MT+, respectively. Our results point to a model in which the two MTs stimulate each other, but the stimulation by the MT− is amplified after MT+ perception in a positive feedback manner.
Social Structure and Temporal Distribution of Tursiops truncatus in the Gulf of Taranto (Central Mediterranean Sea)
Authors: Cipriano Giulia , Santacesaria Francesca Cornelia , Fanizza Carmelo , Cherubini Carla , Crugliano Roberto , Maglietta Rosalia , Ricci Pasquale , Carlucci Roberto
Published: December 2022

This study aims to provide information on the site fidelity, residency patterns and the social structures of bottlenose dolphins occurring in the Gulf of Taranto in order to supply effective indications supporting the future management and conservation measures of the species. Out of 141 photo-identified individuals about 76% were re-sighted from 2 up to 31 times. The site fidelity analysis of photo-identified individuals highlighted the occurrence of 20 seasonal residents, 62 visitors and 59 transient individuals that were included in a local population in which emigration and reimmigration events occurred, as suggested by the residency-pattern analysis. The association pattern, performed using SOCPROG 2.9, highlighted a relatively low mean value of the overall half-weight association index (0.11 ± 0.04). However, the test for the null hypothesis of ‘random association’ was rejected and the temporal analysis made with SLAR suggested the presence of both extremely fluid and stable associations between individuals, describing a fission fusion social structure with a certain degree of social organization. Moreover, the cluster and social network analysis showed two geographically and socially segregated units. Thus, more investigations are needed and the development of a specific conservation plan for bottlenose dolphins in the whole area is required.
Crack Growth in Ni-Cr-Mo-V Steel Using ΔCTOD Elastic–Plastic Model
Authors: Yue Jingxia , Lei Jiankang , Garbatov Yordan , Yang Ke
Published: December 2022

Many studies have shown that the linear elastic fracture mechanics (LEFM) method based on the stress intensity factor range (ΔK) has limitations that cannot be ignored. Due to neglecting the influence of plastic deformation near the crack tip, LEFM shows apparent deviations in evaluating the fracture behaviour. Therefore, in this study, the crack tip opening displacement range (ΔCTOD) is chosen as an alternative to ΔK and based on the elastic–plastic fracture mechanics (EPFM) to develop a new fracture behaviour assessment approach for marine structures. Firstly, a ΔCTOD model based on the HRR (Hutchinson, Rice, and Rosengren) solution is proposed considering the crack closure effect. Secondly, a series of compact tension (CT) specimen crack growth experiments under constant amplitude loading is carried out. According to the experimental results, the prediction accuracy of the HRR model and traditional Irwin and Dugdale models is compared and analysed. The rationality of ΔCTOD as an alternative to ΔK is verified. The results show that ΔCTOD can describe the crack propagation behaviour well. The proposed HRR model shows better accuracy and a more comprehensive application range than the traditional models, which has a guiding significance for studying fracture behaviour for marine structural applications.
Efficient Structural Dynamic Analysis Using Condensed Finite Element Matrices and Its Application to a Stiffened Plate
Authors: Ko Do-Hyun , Boo Seung-Hwan
Published: December 2022

In this study, we propose effective formulations for modal, frequency response, and transient analyses using condensed matrices of the finite element (FE) model. Employing the iterated improved reduced system (IIRS) method, a transformation matrix is defined that condenses the stiffness and inertial effects of nodes to be neglected into nodes of interest. Using this, the condensed mass and stiffness matrices are derived. With these two condensed matrices, a condensed damping matrix is derived using the Rayleigh damping. By considering the condensed matrices in the original structural dynamic formulation based on the global FE matrices, the condensed structural dynamic formulation is derived, and the approximated solutions are calculated from this condensed formulation. To verify the performance of the proposed formulation, we perform a structural dynamic analysis on a stiffened plate, and by comparison with the solutions calculated from the global FE matrices, the proposed formulations have been found to provide highly accurate solutions with an excellent computational efficiency.
Modelling and Simulation Hydrodynamics Processes in Liquefied Natural Gas Transportation Systems
Authors: Bogdevicius Marijonas , Semaskaite Vigaile , Paulauskiene Tatjana , Uebe Jochen , Danilevicius Algimantas
Published: December 2022

Global natural gas resources are growing and are increasingly geographically diverse. A Floating Storage and Regasification Unit (FSRU) is one of the most commonly used vessel types in the global ship fleet due to the possibility of storage, reloading to another ship, and regasifying it for re-injection into the natural gas grid. It is important to control system parameters for reliable technological processes such as tank hydrostatic pressure, vapor pressure, LNG density, LNG temperature, and phase changes between liquid and gas states. Additionally, pressure monitoring is important to control during transit in port and bunkering to prevent the pressure in the tanks from exceeding the tank design pressure. In this research study, a comprehensive hydrodynamic model for an LNG storage tank in a real-life regasification terminal (Floating Storage and Regasification Unit, LNG Terminal of Klaipeda City, Lithuania), operating in transportation mode to the regasification unit, was created. For this research, LNG is investigated as a compressible liquid and the speed of sound in LNG is evaluated. A complex mathematical model of the system allows the analysis of high-speed hydrodynamic and dynamic processes at cryogenic temperature (110 K) and evaluates the geometric parameters (tank geometry, electric motors and pumps, pipe geometric parameters, and roughness of internal surfaces) and the characteristics of pumps and electric motors. The complex mathematical model of the system was implemented using Fortran programing language and MATLAB R28a. It determined the parameters (pressure, velocity, liquid level of LNG in the tanks, electric motor angular velocity, torques, hydraulic energy losses, etc.) of the system during its start-up mode (until 5 s). It was found that hydraulic energy losses in all pipes contain 1.7% of the whole system power (the total power of the electric motors is 3132 kW). In case of increasing energy costs, this model could be used to control energy losses during the operation of the FSRU in various technological modes.
Morphological Characteristics and Phylogeny Reveal Six New Species in Russula Subgenus Russula (Russulaceae, Russulales) from Yanshan Mountains, North China
Authors: Zhou Hao , Cheng Gui-Qiang , Wang Qiu-Tong , Guo Mei-Jun , Zhuo Lan , Yan Hui-Fang , Li Guo-Jie , Hou Cheng-Lin
Published: December 2022

Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide, some of which are famous edible fungi. Although many new species have been described in China, their diversity in North China is still poorly known. Based on the morphology observation of specimens and molecular phylogenetic analyses, combined with the current classification frame of Russula, six new species of Russula subgenus Russula are proposed from the Yanshan Mountains in northern Beijing and northern Hebei Province of China in this study: viz. Russula miyunensis (subsection Chamaeleontinae), R. plana (subsection Chamaeleontinae), R. sinoparva (subsection Puellarinae), R. sinorobusta (subsection Puellarinae), R. subversatilis (subsection Roseinae), and R. yanshanensis (subsection Puellarinae). This is the first report of the species of Russula subgenus Russula from the Yanshan Mountains. This study enriches the species diversity of Russula in North China and provides new data support for the systematic study of Russula in subsequent research, including research and development on edibility.
Prevalence and Associated Factors of Cryptococcal Antigenemia in HIV-Infected Patients with CD4 < 200 Cells/µL in São Paulo, Brazil: A Bayesian Analysis
Authors: Mimicos Evanthia Vetos , Fossaluza Victor , Picone Camila de Melo , de Sena Camila Caroline , Gomes Hélio Rodrigues , Lázari Carolina dos Santos , Silva Fernanda Ferreira da , Nakanishi Erika Shimoda , Nisida Isabelle Vichr , Freitas Angela Carvalho , Gryschek Ronaldo Borges , Lagonegro Eduardo Ronner , Lazéra Márcia , Shikanai-Yasuda Maria Aparecida
Published: December 2022

Cryptococcosis is a severe life-threatening disease and a major cause of mortality in people with advanced AIDS and CD4 ≤ 100 cells/µL. Considering the knowledge gap regarding the benefits of routine application of antigenemia tests in HIV-infected patients with 100–200 CD4 cells/µL for the prevention of cryptococcal meningitis (CM), we aimed to evaluate the prevalence of positive antigenemia through lateral flow assay (LFA) and associated factors in HIV-infected patients with CD4 < 200 cells/µL. Our findings of 3.49% of positive LFA (LFA+) patients with CD4 < 100 cells/µL and 2.24% with CD4 between 100–200 cells/µL have been included in a Bayesian analysis with 12 other studies containing similar samples worldwide. This analysis showed a proportion of 3.6% LFA+ patients (95% credible interval-Ci [2.5–5.7%]) with CD4 < 100 cells/µL and 1.1% (95%Ci [0.5–4.3%]) with CD4 between 100–200 cells/µL, without statistical difference between these groups. The difference between mortality rates in LFA+ and negative LFA groups was e = 0.05013. Cryptococcoma and CM were observed in the LFA+ group with 100–200 and <100 CD4 cells/µL, respectively. Considering the benefits of antifungal therapy for LFA+ patients, our data reinforced the recommendation to apply LFA as a routine test in patients with 100–200 CD4 cells/µL aiming to expand cost-effectiveness studies in this group.
Diagnostic Mycology Laboratories Should Have a Central Role for the Management of Fungal Disease
Authors: Medina Narda , Alastruey-Izquierdo Ana , Mercado Danicela , Denning David W. , Arathoon Eduardo , Rodriguez-Tudela Juan Luis
Published: December 2022

The absence of awareness of fungal diseases as part of the differential diagnosis in at-risk populations has severe consequences. Here, we show how the active role of laboratories can improve patients’ survival. Recently, major advances have been made in non-culture-based assays for fungal diseases, improving accuracy and turnaround time. Furthermore, with the introduction of proficiency control systems, laboratories are an easily monitored environment with good analytical accuracy. Diagnostic packages for opportunistic infections can overcome many deficiencies caused by the absence of awareness. In Guatemala, to make diagnosis accessible, we set up a diagnostic laboratory hub (DLH) providing screening for cryptococcosis, histoplasmosis and tuberculosis to a network of 13 healthcare facilities attending people living with HIV (PLWHIV). In two years, we screened 2127 newly HIV-diagnosed patients. The frequency of opportunistic infections was 21%, rising to 30.3% in patients with advanced HIV disease (<200 CD4); 8.1% of these patients had more than one infection. With the implementation of this diagnostic package, mortality decreased by 7%, a key goal of many public health interventions. Screening for serious infection in high-risk populations can partially overcome training or experiential deficiencies among clinicians for life-threatening fungal diseases.
Antifungal Mechanism of Metabolites from Newly Isolated Streptomyces sp. Y1-14 against Banana Fusarium Wilt Disease Using Metabolomics
Authors: Cao Miaomiao , Cheng Qifeng , Cai Bingyu , Chen Yufeng , Wei Yongzan , Qi Dengfeng , Li Yuqi , Yan Liu , Li Xiaojuan , Long Weiqiang , Liu Qiao , Xie Jianghui , Wang Wei
Published: December 2022

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is one of the most destructive banana diseases in the world, which limits the development of the banana industry. Compared with traditional physical and chemical practices, biological control becomes a promising safe and efficient strategy. In this study, strain Y1-14 with strong antagonistic activity against Foc TR4 was isolated from the rhizosphere soil of a banana plantation, where no disease symptom was detected for more than ten years. The strain was identified as Streptomyces according to the morphological, physiological, and biochemical characteristics and the phylogenetic tree of 16S rRNA. Streptomyces sp. Y1-14 also showed a broad-spectrum antifungal activity against the selected 12 plant pathogenic fungi. Its extracts inhibited the growth and spore germination of Foc TR4 by destroying the integrity of the cell membrane and the ultrastructure of mycelia. Twenty-three compounds were identified by gas chromatography–mass spectrometry (GC-MS). The antifungal mechanism was investigated further by metabolomic analysis. Strain Y1-14 extracts significantly affect the carbohydrate metabolism pathway of Foc TR4 by disrupting energy metabolism.
A Mathematical Formulation of the Valuation of Ether and Ether Derivatives as a Function of Investor Sentiment and Price Jumps
Authors: Abraham Rebecca , El-Chaarani Hani
Published: December 2022

The purpose of this study was to create quantitative models to value ether, ether futures, and ether options based upon the ability of cryptocurrencies to transform existing intermediary-verified payments to non-intermediary-based currency transfers, the ability of ether as a late mover to displace bitcoin as the first mover, and the valuation of ether in the context of investor irrationality models. The risk-averse investor’s utility function is a combination of expectations of the performance of ether, expectations of cryptocurrencies’ transformative power, and expectations of ether superseding bitcoin. The moderate risk-taker’s utility function is an alt-Weibull distribution, along with a gamma distribution. Risk-takers have a utility function in the form of a Bessel function. Ether price functions consist of a Levy jump process. Ether futures are valued as the combination of current spot prices along with term prices. The value of spot prices is the product of a spot premium and a lognormal distribution of spot prices. The value of term prices is equal to the product of a term premium, and the Levy jump process of price fluctuations during the delivery period. For ether options, a less risky ether option portfolio offsets ether’s risk by a fixed-income trading strategy.