bluuebunny/crossref_metadata_2025 huggingface dataset:
Examining the Importance of Green Food in the Restaurant Industry: Focusing on Behavioral Intentions to Eat Insects
Authors: Hwang Jinsoo , Kim Hyunjoon
Published: February 2021

This study analyzed the psychological benefits of environmentally friendly edible insect restaurants, by proposing that three subdimensions of psychological benefits positively affect attitude. Attitude was hypothesized to play an important role in the formation of desire and two subdimensions of behavioral intentions: intentions to use and willingness to pay more. A research model was verified using responses from 419 respondents collected in Korea. Data analysis indicated that (1) warm glow, (2) self-expressive benefits, and (3) nature experiences form attitude and that attitude helps to increase desire, which in turn positively enhances behavioral intentions. The data analysis results supported the importance of the psychological benefits of environmentally friendly edible insect restaurants.
Seroprevalence Study and Cross-Sectional Survey on COVID-19 for a Plan to Reopen the University of Alicante (Spain)
Authors: Tuells Jose , Egoavil Cecilia M. , Pena Pardo María Angeles , Montagud Ana C. , Montagud Emilia , Caballero Pablo , Zapater Pedro , Puig-Barberá Joan , Hurtado-Sanchez Jose Antonio
Published: February 2021

The implementation of strategies to mitigate possible cases of COVID-19 were addressed at the University of Alicante for the safe reopening of the 2020/2021 academic year. To discover the prevalence of immunity against SARS-CoV-2, a study was designed using a rapid immunoassay test (carried out between 6 and 22 July 2020), and in addition a cross-sectional survey was conducted on risk factors, symptoms, predisposition for becoming vaccinated, and sources of information about COVID-19. A random sample, stratified by students, faculty, and administrative staff, was selected. The seroprevalence found was 2.64% (39/1479; 95% CI 1.8–3.4), and the adjusted seroprevalence was 2.89% (95% CI 2.1–3.7). The average age of the students was 23.2 years old, and 47.6 years old for staff. In relation to COVID-19, the following was found: 17.7% pauci-symptomatic, 1.3% symptomatic, 5.5% contact with cases, 4.9% confined, and 0.3% PCR positive. More than 90% complied with preventive measures. The proportion willing to receive the COVID-19 vaccine was 91%. Their sources of information were the Internet (74%) and television (70.1%). They requested that the university offer information (45.1%), training (27%), and provide Personal Protective Equipment (PPE) (26.3%). Lastly, 87.9% would repeat the test. A plan was established that included the follow-up of cases and contacts, random sample testing, training courses, bimodal teaching, a specific website, and the distribution of PPE.
Exploring the Influence of E-Hailing Applications on the Taxi Industry—From the Perspective of the Drivers
Authors: Gan Yitong , Fan Hongchao , Jiao Wei , Sun Mengqi
Published: February 2021

In China, the traditional taxi industry is conforming to the trend of the times, with taxi drivers working with e-hailing applications. This reform is of great significance, not only for the taxi industry, but also for the transportation industry, cities, and society as a whole. Our goal was to analyze the changes in driving behavior since taxi drivers joined e-hailing platforms. Therefore, this paper mined taxi trajectory data from Shanghai and compared the data of May 2015 with those of May 2017 to represent the before-app stage and the full-use stage, respectively. By extracting two-trip events (i.e., vacant trip and occupied trip) and two-spot events (i.e., pick-up spot and drop-off spot), taxi driving behavior changes were analyzed temporally, spatially, and efficiently. The results reveal that e-hailing applications mine more long-distance rides and new pick-up locations for drivers. Moreover, driver initiative have increased at night since using e-hailing applications. Furthermore, mobile payment facilities save time that would otherwise be taken sorting out change. Although e-hailing apps can help citizens get taxis faster, from the driver’s perspective, the apps do not reduce their cruising time. In general, e-hailing software reduces the unoccupied ratio of taxis and improves the operating ratio. Ultimately, new driving behaviors can increase the driver’s revenue. This work is meaningful for the formulation of reasonable traffic laws and for urban traffic decision-making.
Early Enriched Environment Prevents Epigenetic p11 Gene Changes Induced by Adulthood Stress in Mice
Authors: Seo Mi , Choi Ah , Seog Dae-Hyun , Lee Jung , Park Sung
Published: February 2021

Positive experiences in early life may improve the capacity to cope with adulthood stress through epigenetic modification. We investigated whether an enriched environment (EE) in the postnatal period affected epigenetic changes in the p11 gene induced by chronic unpredictable stress (CUS) in adult C57BL/6J mice. EE was introduced for 5 weeks during postnatal days 21–55. After EE, the mice were subjected to CUS for 4 weeks. EE prevented depression-like behavior induced by adult CUS. EE prevented a decrease in p11 mRNA and histone H3 acetylation induced by CUS, with changes in the expression of histone deacetylase 5. Moreover, EE prevented changes in trimethylation of histone H3 lysine 4 (H3K4) and H3K27 induced by CUS. Furthermore, EE had positive effects on behavior and epigenetic alterations in adult mice without CUS. These results suggest that one of the underlying mechanisms of early-life EE may involve epigenetic modification of the hippocampal p11 gene promoter.
Preeclampsia: Cardiotonic Steroids, Fibrosis, Fli1 and Hint to Carcinogenesis
Authors: Agalakova Natalia I. , Kolodkin Nikolai I. , Adair C. David , Trashkov Alexander P. , Bagrov Alexei Y.
Published: February 2021

Despite prophylaxis and attempts to select a therapy, the frequency of preeclampsia does not decrease and it still takes the leading position in the structure of maternal mortality and morbidity worldwide. In this review, we present a new theory of the etiology and pathogenesis of preeclampsia that is based on the interaction of Na/K-ATPase and its endogenous ligands including marinobufagenin. The signaling pathway of marinobufagenin involves an inhibition of transcriptional factor Fli1, a negative regulator of collagen synthesis, followed by the deposition of collagen in the vascular tissues and altered vascular functions. Moreover, in vitro and in vivo neutralization of marinobufagenin is associated with the restoration of Fli1. The inverse relationship between marinobufagenin and Fli1 opens new possibilities in the treatment of cancer; as Fli1 is a proto-oncogene, a hypothesis on the suppression of Fli1 by cardiotonic steroids as a potential anti-tumor therapeutic strategy is discussed as well. We propose a novel therapy of preeclampsia that is based on immunoneutralization of the marinobufagenin by monoclonal antibodies, which is capable of impairing marinobufagenin-Na/K-ATPase interactions.
Pharmacogenomic Profile and Adverse Drug Reactions in a Prospective Therapeutic Cohort of Chagas Disease Patients Treated with Benznidazole
Authors: Franco Lucas A. M. , Moreira Carlos H. V. , Buss Lewis F. , Oliveira Lea C. , Martins Roberta C. R. , Manuli Erika R. , Lindoso José A. L. , Busch Michael P. , Pereira Alexandre C. , Sabino Ester C.
Published: February 2021

Chagas disease remains a major social and public health problem in Latin America. Benznidazole (BZN) is the main drug with activity against Trypanosoma cruzi. Due to the high number of adverse drug reactions (ADRs), BZN is underprescribed. The goal of this study was to evaluate the genetic and transcriptional basis of BZN adverse reactions. Methods: A prospective cohort with 102 Chagas disease patients who underwent BZN treatment was established to identify ADRs and understand their genetic basis. The patients were classified into two groups: those with at least one ADR (n = 73), and those without ADRs (n = 29). Genomic analyses were performed comparing single nucleotide polymorphisms between groups. Transcriptome data were obtained comparing groups before and after treatment, and signaling pathways related to the main ADRs were evaluated. Results: A total of 73 subjects (71.5%) experienced ADRs. Dermatological symptoms were most frequent (45.1%). One region of chromosome 16, at the gene LOC102724084 (rs1518601, rs11861761, and rs34091595), was associated with ADRs (p = 5.652 × 10−8). Transcriptomic data revealed three significantly enriched signaling pathways related to BZN ADRs. Conclusions: These data suggest that part of adverse BZN reactions might be genetically determined and may facilitate patient risk stratification prior to starting BZN treatment.
Device Discovery and Context Registration in Static Context Header Compression Networks
Authors: Moons Bart , De Poorter Eli , Hoebeke Jeroen
Published: February 2021

Due to the limited bandwidth of Low-Power Wide-Area Networks (LPWAN), the application layer is currently often tied straight above the link layer, limiting the evolution of sensor networks distributed over a large area. Consequently, the highly efficient Static Context Header Compression (SCHC) standard was introduced, where devices can compress the IPv6 and upper layer protocols down to a single byte. This approach, however, assumes that every compression context is distributed before deployment, again limiting the evolution of such networks. Therefore, this paper presents two context registration mechanisms leveraging on the SCHC adaptation layer. This is done by analyzing current registration solutions in order to find limitations and optimizations with regard to very constrained networks. Both solutions and the current State-of-The-Art (SoTA) are evaluated in a Lightweight Machine to Machine (LwM2M) environment. In such situation, both developed solutions decrease the energy consumption already after 25 transmissions, compared with the current SoTA. Furthermore, simulations show that Long Range (LoRa) devices still have a 80% chance to successfully complete the registration flow in a network with a 50% Packet Error Ratio. Briefly, the work presented in this paper delivers bootstrapping tools to constrained, SCHC-enabled networks while still being able to reduce energy consumption.
Calprotectin, an Emerging Biomarker of Interest in COVID-19: A Systematic Review and Meta-Analysis
Authors: Udeh Raphael , Advani Shailesh , de Guadiana Romualdo Luis García , Dolja-Gore Xenia
Published: February 2021

COVID-19 has been shown to present with varied clinical course, necessitating a need for more specific diagnostic tools that could identify severe cases and predict outcomes during COVID-19 infection. Recent evidence has shown an expanded potential role for calprotectin, both as a diagnostic tool and also as a tool in stratifying COVID-19 patients in terms of severity. Therefore, this systematic review and meta-analysis aims to evaluate the levels of calprotectin in severe and non-severe COVID-19 and also identify the implication of raised calprotectin levels. MEDLINE, EMBASE, The Cochrane Library, Web of science and MedRxiv were searched. Meta-analysis was done to compare the serum/fecal levels of calprotectin between severe and non-severe COVID-19 infections. A total of ten studies included in the review (eight had quantitative data while two were qualitative). A pooled analysis of the eight studies from 613 patients who were RT-PCR positive for COVID-19 (average age = 55 years; 52% males) showed an overall estimate as 1.34 (95%CI: 0.77, 1.91). In conclusion, calprotectin levels have been demonstrated to be significantly elevated in COVID-19 patients who develop the severe form of the disease, and it also has prognostic importance.
Otosurgery with the High-Definition Three-Dimensional (3D) Exoscope: Advantages and Disadvantages
Authors: Wierzbicka Małgorzata , Szyfter Witold , Greczka Grażyna , Gawęcki Wojciech
Published: February 2021

Background: The aim of the study was to describe our initial experience with the high-definition three-dimensional (3D) exoscope for middle ear surgery versus the operating microscope. Methods: The study included 60 randomly chosen patients diagnosed with otosclerosis (n = 30) or chronic otitis media (n = 30) with a clinical indication for surgery. The primary measurement was the subjective estimation of quality of the visibility of the operating field provided by the 3D exoscope—VITOM-3D (Karl Storz, Tuttlingen, Germany) in comparison to the operating microscope. Results: All procedures, except for two (3.3%) converted to the microscope, were successfully completed using a 3D exoscope. In both stapedotomy and tympanoplasty, the exoscope was superior to the microscope during more superficial portions of the procedures. By contrast, in deeper areas of the middle ear, the exoscope provided significantly worse visibility, but usually not suboptimal. Both intraoperative bleeding and the narrow surgical field substantially reduced the visibility with the 3D exoscope in comparison to the microscope. Conclusions: Overall, our study shows that the 3D exoscope offers excellent, highly magnified, and well-illuminated high-definition images of the surgical field. However, our experience revealed several important limitations of this system, including decreased depth perception in deep areas of the tympanic cavity and reduced visibility in a difficult surgical field, with subsequent need to switch to an operating microscope in select cases.
Intra-Aortic Balloon Pump and Ischemic Cardiogenic Shock May Still Be a Valuable Association
Authors: Rey Florian , Giraud Raphaël , Bendjelid Karim
Published: February 2021

The IABP gives rise to greater myocardial perfusion by increasing the coronary pressure gradient from the aorta to the coronary circulation at a time when the aortic valve is closed [...]
Renal Denervation for Uncontrolled and Resistant Hypertension: Systematic Review and Network Meta-Analysis of Randomized Trials
Authors: Silverwatch Jonathan , Marti Kristen E. , Phan Mi T. , Amin Hinali , Roman Yuani M. , Pasupuleti Vinay , Banach Maciej , Barboza Joshuan J. , Hernandez Adrian V.
Published: February 2021

Comparative efficacy and safety of renal denervation (RDN) interventions for uncontrolled (UH) and resistant hypertension (RH) is unknown. We assessed the comparative efficacy and safety of existing RDN interventions for UH and RH. Six search engines were searched up to 1 May 2020. Primary outcomes were mean 24-h ambulatory and office systolic blood pressure (SBP). Secondary outcomes were mean 24-h ambulatory and office diastolic blood pressure (DBP), clinical outcomes, and serious adverse events. Frequentist random-effects network meta-analyses were used to evaluate effects of RDN interventions. Twenty randomized controlled trials (RCTs) (n = 2152) were included, 15 in RH (n = 1544) and five in UH (n = 608). Intervention arms included radiofrequency (RF) in main renal artery (MRA) (n = 10), RF in MRA and branches (n = 4), RF in MRA+ antihypertensive therapy (AHT) (n = 5), ultrasound (US) in MRA (n = 3), sham (n = 8), and AHT (n = 9). RF in MRA and branches ranked as the best treatment to reduce 24-h ambulatory, daytime, and nighttime SBP and DBP versus other interventions (p-scores: 0.83 to 0.97); significant blood pressure effects were found versus sham or AHT. RF in MRA+AHT was the best treatment to reduce office SBP and DBP (p-scores: 0.84 and 0.90, respectively). RF in MRA and branches was the most efficacious versus other interventions to reduce 24-h ambulatory SBP and DBP in UH or RH.
Thrombin Is an Effective and Safe Therapy in the Management of Bleeding Gastric Varices. A Real-World Experience
Authors: Gillespie Sarah-Louise , McAvoy Norma C. , Yung Diana E. , Robertson Alexander , Plevris John N. , Hayes Peter C.
Published: February 2021

Variceal haemorrhage is a severe complication of liver disease with high mortality. Human recombinant thrombin has gained popularity in the management of variceal haemorrhage. We report on the use of thrombin for gastric and ectopic varices at a regional tertiary care centre. This was a retrospective observational study. Patients with portal hypertension who received endoscopic injection of recombinant thrombin were identified and data collected on haemostasis and rebleeding rates, complications and mortality. Patients were grouped by indication for thrombin injection: gastric/oesophageal/ectopic varices and endoscopic band ligation (EBL)-induced ulceration. 155 patients (96M/59F, mean age 58.3 years) received endoscopic thrombin injection. Mean volume of thrombin injected at index endoscopy was 9.5 ml/2375IU. Initial haemostasis was achieved in 144 patients (92.9%). Rebleeding occurred in a total of 53 patients (36.8%) divided as follows: early rebleeding (<5 days from index endoscopy)—26 patients (18%); rebleeding within 30 days—42 patients (29.1%); delayed rebleeding (> 30 days)—11 patients (7.6%). There was statistically significant difference in rate of initial haemostasis between Child-Pugh A/B patients vs Child-Pugh C (p = 0.046). There was no significant difference in rebleeding rates between different indication groups (p = 0.78), by presence of cirrhosis or by Child-Pugh Score. All-cause mortality at 6 weeks was 18.7%; 1-year mortality 37.4% (median follow-up 48 months). There was no significant difference in mortality between groups (p = 0.37). No significant adverse events or complications were reported. Thrombin is effective and safe for gastric varices and other portal-hypertension-related bleeding including oesophageal varices, ulcers secondary to EBL and ectopic varices.
Investigating the Effect of Heterogeneous Hull Roughness on Ship Resistance Using CFD
Authors: Song Soonseok , Demirel Yigit Kemal , De Marco Muscat-Fenech Claire , Sant Tonio , Villa Diego , Tezdogan Tahsin , Incecik Atilla
Published: February 2021

Research into the effects of hull roughness on ship resistance and propulsion is well established, however, the effect of heterogeneous hull roughness is not yet fully understood. In this study, Computational Fluid Dynamics (CFD) simulations were conducted to investigate the effect of heterogeneous hull roughness on ship resistance. The Wigley hull was modelled with various hull conditions, including homogeneous and heterogeneous hull conditions. The results were compared against existing experimental data and showed a good agreement, suggesting that the CFD approach is valid for predicting the effect of heterogeneous hull roughness on ship resistance. Furthermore, the local distributions of the wall shear stress and roughness Reynolds number on the hull surface were examined to assess the flow characteristics over the heterogeneous hull roughness.
Formation of Thiophene under Simulated Volcanic Hydrothermal Conditions on Earth—Implications for Early Life on Extraterrestrial Planets?
Authors: Geisberger Thomas , Sobotta Jessica , Eisenreich Wolfgang , Huber Claudia
Published: February 2021

Thiophene was detected on Mars during the Curiosity mission in 2018. The compound was even suggested as a biomarker due to its possible origin from diagenesis or pyrolysis of biological material. In the laboratory, thiophene can be synthesized at 400 °C by reacting acetylene and hydrogen sulfide on alumina. We here show that thiophene and thiophene derivatives are also formed abiotically from acetylene and transition metal sulfides such as NiS, CoS and FeS under simulated volcanic, hydrothermal conditions on Early Earth. Exactly the same conditions were reported earlier to have yielded a plethora of organic molecules including fatty acids and other components of extant metabolism. It is therefore tempting to suggest that thiophenes from abiotic formation could indicate sites and conditions well-suited for the evolution of metabolism and potentially for the origin-of-life on extraterrestrial planets.
Tribological Performance of Additively Manufactured AISI H13 Steel in Different Surface Conditions
Authors: Guenther Elisabeth , Kahlert Moritz , Vollmer Malte , Niendorf Thomas , Greiner Christian
Published: February 2021

Additive manufacturing of metallic tribological components offers unprecedented degrees of freedom, but the surface roughness of most as-printed surfaces impedes the direct applicability of such structures, and postprocessing is necessary. Here, the tribological performance of AISI H13 steel samples was studied. These were additively manufactured through laser powder bed fusion (L-PBF), also referred to as selective laser melting (SLM). Samples were tested in four different surface conditions: as-printed, polished, ground and polished, and laser-surface-textured (LST) with round dimples. Friction experiments were conducted in a pin-on-disk fashion against bearing steel disks under lubrication with an additive-free mineral base oil for sliding speeds between 20 and 170 mm/s. Results demonstrated that, among the four surface treatments, grinding and polishing resulted in the lowest friction coefficient, followed by the as-printed state, while both polishing alone and laser-surface texturing increased the friction coefficient. Surprisingly, direct correlation between surface roughness and friction coefficient, i.e., the rougher the surface was, the higher the friction force, was not observed. Wear was minimal in all cases and below what could be detected by gravimetrical means. These results highlight the need for an adequate post-processing treatment of additively manufactured parts that are to be employed in tribological systems.
Textile Materials Modified with Stimuli-Responsive Drug Carrier for Skin Topical and Transdermal Delivery
Authors: Atanasova Daniela , Staneva Desislava , Grabchev Ivo
Published: February 2021

Textile materials, as a suitable matrix for different active substances facilitating their gradual release, can have an important role in skin topical or transdermal therapy. Characterized by compositional and structural variety, those materials readily meet the requirements for applications in specific therapies. Aromatherapy, antimicrobial substances and painkillers, hormone therapy, psoriasis treatment, atopic dermatitis, melanoma, etc., are some of the areas where textiles can be used as carriers. There are versatile optional methods for loading the biologically active substances onto textile materials. The oldest ones are by exhaustion, spraying, and a pad-dry-cure method. Another widespread method is the microencapsulation. The modification of textile materials with stimuli-responsive polymers is a perspective route to obtaining new textiles of improved multifunctional properties and intelligent response. In recent years, research has focused on new structures such as dendrimers, polymer micelles, liposomes, polymer nanoparticles, and hydrogels. Numerous functional groups and the ability to encapsulate different substances define dendrimer molecules as promising carriers for drug delivery. Hydrogels are also high molecular hydrophilic structures that can be used to modify textile material. They absorb a large amount of water or biological fluids and can support the delivery of medicines. These characteristics correspond to one of the current trends in the development of materials used in transdermal therapy, namely production of intelligent materials, i.e., such that allow controlled concentration and time delivery of the active substance and simultaneous visualization of the process, which can only be achieved with appropriate and purposeful modification of the textile material.
Electrospun Membranes Based on Polycaprolactone, Nano-Hydroxyapatite and Metronidazole
Authors: Mirică Ioana-Codruţa , Furtos Gabriel , Lucaciu Ondine , Pascuta Petru , Vlassa Mihaela , Moldovan Mărioara , Campian Radu-Septimiu
Published: February 2021

The aim of this research was to develop new electrospun membranes (EMs) based on polycaprolactone (PCL) with or without metronidazole (MET)/nano-hydroxyapatite (nHAP) content. New nHAP with a mean diameter of 34 nm in length was synthesized. X-ray diffraction (XRD) and attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) were used for structural characterization of precursors and EMs. The highest mechanical properties (the force at maximum load, Young’s modulus and tensile strength) were found for the PCL membranes, and these properties decreased for the other samples in the following order: 95% PCL + 5% nHAP > 80% PCL + 20% MET > 75% PCL + 5% nHAP + 20% MET. The stiffness increased with the addition of 5 wt.% nHAP. The SEM images of EMs showed randomly oriented bead-free fibers that generated a porous structure with interconnected macropores. The fiber diameter showed values between 2 and 16 µm. The fiber diameter increased with the addition of nHAP filler and decreased when MET was added. New EMs with nHAP and MET could be promising materials for guided bone regeneration or tissue engineering.
Composites of Open-Cell Viscoelastic Foams with Blackcurrant Pomace
Authors: Auguścik-Królikowska Monika , Ryszkowska Joanna , Kurańska Maria , Wantulok Marta , Gloc Michał , Szczepkowski Leonard , Dąbkowska-Susfał Katarzyna , Prociak Aleksander
Published: February 2021

Taking into account the circular economy guidelines and results of life cycle analyses of various materials, it was proposed to use a blackcurrant pomace filler in the production process of viscoelastic polyurethane (PUR) foams intended for application as mattresses, pillows, or elements for orthopedics. Open-cell viscoelastic PUR foams containing 10–60 per hundred polyols (php) blackcurrant pomace were prepared. It was found that after introducing the filler to the PUR foam formulation, the speed of the first stage of the foaming process significantly decreases, the maximum temperature achieved during the synthesis drops (by 30 °C for the foam containing 40 php of filler compared to unfilled foam), and the maximum pressure achieved during the synthesis of foam containing 20 php is reduced by approximately 57% compared to the foam without filler. The growth time of the foams increases with increasing the amount of introduced filler; for the foam containing 60 php, the time is extended even by about 24%. The effect of the filler on the physical, morphological, mechanical, and functional performances of PUR foam composites has been analyzed. The use of 60 php as the filler reduced the hardness of the foams by approximately 30% and increased their comfort factor from 3 to 5.
Study on the Expansion Dynamics of MDCK Epithelium by Interstitial Flow Using a Traction Force-Measurable Microfluidic Chip
Authors: Kim Mirim , Jang Hwanseok , Park Yongdoo
Published: February 2021

The movement of collective cells is affected through changes in physical interactions of cells in response to external mechanical stimuli, including fluid flow. Most tissues are affected by fluid flow at the interstitial level, but few studies have investigated the physical effects in collective cells affected by a low flow rate. In this study, collective cell migration of Madin–Darby canine kidney (MDCK) epithelial cells was investigated under static or interstitial flow (0, 0.1, and 1 μL/min) using a traction microfluidic device. The optimization of calculation of cellular traction forces was first achieved by changing interrogation window size from the fluorescent bead images. Migration analysis of cell collectives patterned with a 700 μm circular shape reveals that cells under the slow flow (0.1 and 1 μL/min) showed the inhibitory migration by decreasing cell island size and cellular speed compared to that of static condition. Analysis of cellular forces shows that level of traction forces was lower in the slow flow condition (~20 Pa) compared to that of static condition (~50 Pa). Interestingly, the standard deviation of traction force of cells was dramatically decreased as the flow rate increased from 0 to 1 μL/min, which indicates that flow affects the distribution of cellular traction forces among cell collectives. Cellular tension was increased by 50% in the cells under the fluid flow rate of 1 μL/min. Treatment of calcium blocker increased the migratory speed of cells under the flow condition, whereas there is little change of cellular forces. In conclusion, it has been shown that the interstitial flow inhibited the collective movement of epithelial cells by decreasing and re-distributing cellular forces. These findings provide insights into the study of the effect of interstitial flow on cellular behavior, such as development, regeneration, and morphogenesis.
On the Benefits of Using Object-Oriented Programming for the Objective Evaluation of Vehicle Dynamic Performance in Concurrent Simulations
Authors: Perrelli Michele , Cosco Francesco , Carbone Giuseppe , Lenzo Basilio , Mundo Domenico
Published: February 2021

Assessing passenger cars’ dynamic performance is a critical aspect for car industries, due to its impact on the overall vehicle safety evaluation and the subjective nature of the involved handling and comfort metrics. Accordingly, ISO standards, such as ISO 4138 and ISO 3888, define several specific driving tests to assess vehicle dynamics performance objectively. Consequently, proper evaluation of the dynamic behaviour requires measuring several physical quantities, including accelerations, speed, and linear and angular displacements obtained after instrumenting a vehicle with multiple sensors. This experimental activity is highly demanding in terms of hardware costs, and it is also significantly time-consuming. Several approaches can be considered for reducing vehicle development time. In particular, simulation software can be exploited to predict the approximate behaviour of a vehicle using virtual scenarios. Moreover, motion platforms and detail-scalable numerical vehicle models are widely implemented for the purpose. This paper focuses on a customized simulation environment developed in C++, which exploits the advantages of object-oriented programming. The presented framework strives to perform concurrent simulations of vehicles with different characteristics such as mass, tyres, engine, suspension, and transmission systems. Within the proposed simulation framework, we adopted a hierarchical and modular representation. Vehicles are modelled by a 14 degree-of-freedom (DOF) full-vehicle model, capable of capturing the dynamics and complemented by a set of scalable-detail models for the remaining sub-systems such as tyre, engine, and steering system. Furthermore, this paper proposes the usage of autonomous virtual drivers for a more objective evaluation of vehicle dynamic performances. Moreover, to further evaluate our simulator architecture’s efficiency and assess the achieved level of concurrency, we designed a benchmark able to analyse the scaling of the performances with respect to the number of different vehicles during the same simulation. Finally, the paper reports the proposed simulation environment’s scalability resulting from a set of different and varying driving scenarios.
Estimating Equations for Density Dependent Markov Jump Processes
Authors: Odubote Oluseyi , Linder Daniel F.
Published: February 2021

Reaction networks are important tools for modeling a variety of biological phenomena across a wide range of scales, for example as models of gene regulation within a cell or infectious disease outbreaks in a population. Hence, calibrating these models to observed data is useful for predicting future system behavior. However, the statistical estimation of the parameters of reaction networks is often challenging due to intractable likelihoods. Here we explore estimating equations to estimate the reaction rate parameters of density dependent Markov jump processes (DDMJP). The variance–covariance weights we propose to use in the estimating equations are obtained from an approximating process, derived from the Fokker–Planck approximation of the chemical master equation for stochastic reaction networks. We investigate the performance of the proposed methodology in a simulation study of the Lotka–Volterra predator–prey model and by fitting a susceptible, infectious, removed (SIR) model to real data from the historical plague outbreak in Eyam, England.
Application of Hexagonal Fuzzy MCDM Methodology for Site Selection of Electric Vehicle Charging Station
Authors: Ghosh Arijit , Ghorui Neha , Mondal Sankar Prasad , Kumari Suchitra , Mondal Biraj Kanti , Das Aditya , Gupta Mahananda Sen
Published: February 2021

In this paper, the application of hexagonal fuzzy multiple-criteria decision-making (MCDM) methodology for the site selection of electric vehicle charging stations is considered. In this regard, four factors and thirteen sub-factors have been taken into consideration for E-vehicle charging site selection. In this research, the geographic information system (GIS) has been incorporated with MCDM techniques. The fuzzy analytic hierarchy process (FAHP) is used to obtain a fuzzy weight of factors and sub-factors. MCDM tools fuzzy technique for order of preference by similarity to ideal solution (FTOPSIS) and fuzzy complex proportional assessment (FCOPRAS) have been used to rank the selected sites. A centroid-based method for defuzzification and distance measure between two hexagonal fuzzy numbers (HFN) has been developed for this paper. A practical example in Howrah, India, is considered to show the applicability and usefulness of the model. The results depict the suitability of the proposed research. Comparative and sensitivity analyses have been demonstrated to check the reliability, robustness and effectiveness of the proposed method.
A Review on Polymer Nanocomposites and Their Effective Applications in Membranes and Adsorbents for Water Treatment and Gas Separation
Authors: Agboola Oluranti , Fayomi Ojo Sunday Isaac , Ayodeji Ayoola , Ayeni Augustine Omoniyi , Alagbe Edith E. , Sanni Samuel E. , Okoro Emmanuel E. , Moropeng Lucey , Sadiku Rotimi , Kupolati Kehinde Williams , Oni Babalola Aisosa
Published: February 2021

Globally, environmental challenges have been recognised as a matter of concern. Among these challenges are the reduced availability and quality of drinking water, and greenhouse gases that give rise to change in climate by entrapping heat, which result in respirational illness from smog and air pollution. Globally, the rate of demand for the use of freshwater has outgrown the rate of population increase; as the rapid growth in town and cities place a huge pressure on neighbouring water resources. Besides, the rapid growth in anthropogenic activities, such as the generation of energy and its conveyance, release carbon dioxide and other greenhouse gases, warming the planet. Polymer nanocomposite has played a significant role in finding solutions to current environmental problems. It has found interest due to its high potential for the reduction of gas emission, and elimination of pollutants, heavy metals, dyes, and oil in wastewater. The revolution of integrating developed novel nanomaterials such as nanoparticles, carbon nanotubes, nanofibers and activated carbon, in polymers, have instigated revitalizing and favourable inventive nanotechnologies for the treatment of wastewater and gas separation. This review discusses the effective employment of polymer nanocomposites for environmental utilizations. Polymer nanocomposite membranes for wastewater treatment and gas separation were reviewed together with their mechanisms. The use of polymer nanocomposites as an adsorbent for toxic metals ions removal and an adsorbent for dye removal were also discussed, together with the mechanism of the adsorption process. Patents in the utilization of innovative polymeric nanocomposite membranes for environmental utilizations were discussed.
In Vitro Reassortment between Endemic Bluetongue Viruses Features Global Shifts in Segment Frequencies and Preferred Segment Combinations
Authors: Kopanke Jennifer , Lee Justin , Stenglein Mark , Mayo Christie
Published: February 2021

Bluetongue virus (BTV) is an arthropod-borne pathogen that is associated with sometimes severe disease in both domestic and wild ruminants. Predominantly transmitted by Culicoides spp. biting midges, BTV is composed of a segmented, double-stranded RNA genome. Vector expansion and viral genetic changes, such as reassortment between BTV strains, have been implicated as potential drivers of ongoing BTV expansion into previously BTV-free regions. We used an in vitro system to investigate the extent and flexibility of reassortment that can occur between two BTV strains that are considered enzootic to the USA, BTV-2 and BTV-10. Whole genome sequencing (WGS) was coupled with plaque isolation and a novel, amplicon-based sequencing approach to quantitate the viral genetic diversity generated across multiple generations of in vitro propagation. We found that BTV-2 and BTV-10 were able to reassort across multiple segments, but that a preferred BTV-2 viral backbone emerged in later passages and that certain segments were more likely to be found in reassortant progeny. Our findings indicate that there may be preferred segment combinations that emerge during BTV reassortment. Moreover, our work demonstrates the usefulness of WGS and amplicon-based sequencing approaches to improve understanding of the dynamics of reassortment among segmented viruses such as BTV.
Secondary Sulfates from the Monte Arsiccio Mine (Apuan Alps, Tuscany, Italy): Trace-Element Budget and Role in the Formation of Acid Mine Drainage
Authors: D’Orazio Massimo , Mauro Daniela , Valerio Marta , Biagioni Cristian
Published: February 2021

A suite of sulfate minerals from the Monte Arsiccio mine (Apuan Alps, Northern Tuscany, Italy), previously identified by using both X-ray diffraction and micro-Raman spectroscopy, was studied through inductively coupled plasma mass spectrometry (ICP-MS), in order to determine their trace-element content. Several elements (Tl, Rb, As, Sb, Co, Ni, Cu, Zn, and Cr) were found above the detection limits. Among them, some are important from an environmental perspective and may reach relatively high concentrations (e.g., Tl = 1370–2988 μg/g; As = 505–1680 μg/g). Thus, these sulfates may act as transient sinks for some of these potentially toxic elements, as well as for sulfate ions and acidity. Indeed, dissolution experiments revealed the ability of these secondary minerals to produce a significant pH decrease of the solutions, as well as the release of Fe, Al, and K as major ions. This work discusses the relation between the budget of trace elements and the crystal chemistry of sulfate minerals and provides new insights about the environmental role played by the sulfate dissolution in controlling the quality of water in acid mine drainage systems.
Synthesis and Characterization of Chitosan-Based Nanodelivery Systems to Enhance the Anticancer Effect of Sorafenib Drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma Cells
Authors: Ruman Umme , Buskaran Kalaivani , Pastorin Giorgia , Masarudin Mas Jaffri , Fakurazi Sharida , Hussein Mohd Zobir
Published: February 2021

The formation of two nanodelivery systems, Sorafenib (SF)-loaded chitosan (SF-CS) and their folate-coated (SF-CS-FA) nanoparticles (NPs), were developed to enhance SF drug delivery on human Hepatocellular Carcinoma (HepG2) and Colorectal Adenocarcinoma (HT29) cell lines. The ionic gelation method was adopted to synthesize the NPs. The characterizations were performed by DLS, FESEM, TEM, XRD, TGA, FTIR, and UV-visible spectroscopy. It was found that 83.7 ± 2.4% and 87.9 ± 1.1% of encapsulation efficiency; 18.2 ± 1.3% and 19.9 ± 1.4% of loading content; 76.3 ± 13.7 nm and 81.6 ± 12.9 nm of hydrodynamic size; 60–80 nm and 70–100 nm of TEM; and FESEM sizes of near-spherical shape were observed, respectively, for SF-CS and SF-CS-FA nanoparticles. The SF showed excellent release from the nanoparticles under pH 4.8 PBS solution, indicating a good delivery system for tumor cells. The cytotoxicity study revealed their better anticancer action towards HepG2 and HT29 cell lines compared to the free sorafenib. Moreover, both NPs systems showed negligible toxicity to normal Human Dermal Fibroblast adult cells (HDFa). This is towards an enhanced anticancer drug delivery system with sustained-release properties for better cancer management.
Energy Expenditure Improved Risk Factors Associated with Renal Function Loss in NAFLD and MetS Patients
Authors: Abbate Manuela , Mascaró Catalina M. , Montemayor Sofía , Barbería-Latasa María , Casares Miguel , Gómez Cristina , Angullo-Martinez Escarlata , Tejada Silvia , Abete Itziar , Zulet Maria Angeles , Sureda Antoni , Martínez J. Alfredo , Tur Josep A.
Published: February 2021

To assess the efficacy of three lifestyle interventions on the reduction of liver fat content and metabolic syndrome (MetS), and whether such reductions would influence renal outcomes, we conducted a randomized controlled trial on 128 participants with MetS and non-alcoholic fatty liver disease (NAFLD), as well as available data on estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatine ratio (UACR). Patients were randomized in 1:1:1 ratio to either Conventional Diet, Mediterranean diet (MD)–high meal frequency, and MD–physical activity groups. Each intervention aimed at reducing caloric intake by 25%–30% of baseline intake and increase energy expenditure by 400 kcal/70 kg. Patients attended regular visits and were followed-up for 6 months. Increased albuminuria was present in 13.3% of patients, while 32.8% showed hyperfiltration. UACR reduction was associated with higher levels of UACR at baseline but not with changes in liver fat. eGFR decreased in patients presenting hyperfiltration at baseline and was associated with reduction in liver fat and insulin resistance, as well as with increase in energy expenditure (R2 = 0.248, p = 0.006). No significant differences were observed between the three treatment groups. In patients with NAFLD and MetS, energy expenditure significantly reduced hepatic fat accumulation and insulin resistance, which reduced glomerular hyperfiltration. Increased albuminuria was reduced, but it was not associated with reduced liver fat.
Gut Microbiota Bacterial Species Associated with Mediterranean Diet-Related Food Groups in a Northern Spanish Population
Authors: Rosés Carles , Cuevas-Sierra Amanda , Quintana Salvador , Riezu-Boj José I. , Martínez J. Alfredo , Milagro Fermín I. , Barceló Anna
Published: February 2021

The MD (Mediterranean diet) is recognized as one of the healthiest diets worldwide and is associated with the prevention of cardiovascular and metabolic diseases. Dietary habits are considered one of the strongest modulators of gut microbiota, which seem to play a significant role in health status of the host. The purpose of the present study was to evaluate interactive associations between gut microbiota composition and habitual dietary intake in 360 Spanish adults from the Obekit cohort (normal weight, overweight, and obese participants). Dietary intake and adherence to the MD tests were administered and fecal samples were collected from each participant. Fecal 16S rRNA (ribosomal Ribonucleic Acid) gene sequencing was performed and checked against the dietary habits. MetagenomeSeq was the statistical tool applied to analyze data at the species taxonomic level. Results from this study identified several beneficial bacteria that were more abundant in the individuals with higher adherence to the MD. Bifidobacterium animalis was the species with the strongest association with the MD. Some SCFA (Short Chain Fatty Acids) -producing bacteria were also associated with MD. In conclusion, this study showed that MD, fiber, legumes, vegetable, fruit, and nut intake are associated with an increase in butyrate-producing taxa such as Roseburia faecis, Ruminococcus bromii, and Oscillospira (Flavonifractor) plautii.
Functional Food Components, Intestinal Permeability and Inflammatory Markers in Patients with Inflammatory Bowel Disease
Authors: Lacerda Joana Franco , Lagos Ana Catarina , Carolino Elisabete , Silva-Herdade Ana Santos , Silva Manuel , Sousa Guerreiro Catarina
Published: February 2021

Inflammatory bowel diseases (IBD) are characterized by a chronic inflammatory process that affects the intestinal barrier structure. Recent evidence suggests that some food components can influence the integrity of the intestinal barrier and thus its permeability. We aimed at assessing the effect of food components on the intestinal permeability (IP) and on inflammatory markers in individuals with IBD by a single-blind randomized clinical study. Of the 53 individuals included, 47% (n = 25) had been diagnosed with IBD. The participants were divided into 4 groups. IBD patients were allocated to intervention group (n = 14) vs. no intervention group (n = 11), and the same happened with 28 control participants without disease (n = 14 in intervention group vs. n = 14 without intervention). Symptomatology, nutritional status, biochemical parameters (specifically serum zonulin (ZO) to measure IP) were evaluated on all individuals on an eight week period following a diet plan with/without potentially beneficial foods for the IP. At the beginning of the study, there were no significant differences in ZO values between individuals with and without IBD (p > 0.05). The effect of specific food components was inconclusive; however, a trend in the reduction of inflammatory parameters and on the prevalence of gastrointestinal symptomatology was observed. More controlled intervention studies with diet plans, including food components potentially beneficial for the integrity of the intestinal barrier, are of the utmost importance.
Microbiota and Drug Response in Inflammatory Bowel Disease
Authors: Franzin Martina , Stefančič Katja , Lucafò Marianna , Decorti Giuliana , Stocco Gabriele
Published: February 2021

A mutualistic relationship between the composition, function and activity of the gut microbiota (GM) and the host exists, and the alteration of GM, sometimes referred as dysbiosis, is involved in various immune-mediated diseases, including inflammatory bowel disease (IBD). Accumulating evidence suggests that the GM is able to influence the efficacy of the pharmacological therapy of IBD and to predict whether individuals will respond to treatment. Additionally, the drugs used to treat IBD can modualate the microbial composition. The review aims to investigate the impact of the GM on the pharmacological therapy of IBD and vice versa. The GM resulted in an increase or decrease in therapeutic responses to treatment, but also to biotransform drugs to toxic metabolites. In particular, the baseline GM composition can help to predict if patients will respond to the IBD treatment with biologic drugs. On the other hand, drugs can affect the GM by incrementing or reducing its diversity and richness. Therefore, the relationship between the GM and drugs used in the treatment of IBD can be either beneficial or disadvantageous.