bluuebunny/crossref_metadata_2025 huggingface dataset:
How Threatened Is Scincella huanrenensis? An Update on Threats and Trends
Authors: Shin Yucheol , Messenger Kevin R. , Koo Kyo Soung , Lee Sang Cheol , Hou Mian , Borzée Amaël
Published: March 2021

It is important to understand the dynamics of population size to accurately assess threats and implement conservation activities when required. However, inaccurate estimates are harming both the threat estimation process, and the resulting conservation actions. Here, we address the extinction threats to Scincella huanrenensis, a species described in the People’s Republic of China, but also occurring on the Korean peninsula. Estimating the threats to the species is not an easy task due to its unknown population status in the Democratic People’s Republic of Korea. Here we analysed the literature to acquire the known presence point for the species, along with datapoints originating from opportunistic field surveys, and employed habitat suitability models to estimate the range of the species. We then followed the categories and criteria of the IUCN Red List of Threatened Species to assess the extinction risk of the species. We found the species not to be fitting the threatened category at the global scale based on the range size, the only category for which enough data was available. We recommend the status of the species on the IUCN Red List of Threatened Species to be updated as it is now listed as critically endangered (CR), a listing fitting a national assessment for the People’s Republic (PR) of China. While this species is possibly less threatened than currently listed, this is not a genuine improvement, and specific conservation aspects should not be neglected due to its specialisation to medium to high elevation habitat.
Periorbital Hyperpigmentation—Dark Circles under the Eyes; Treatment Suggestions and Combining Procedures
Authors: Goldman Alberto , Goldust Mohamad , Wollina Uwe
Published: March 2021

Periorbital hyperpigmentation (POH) is a frequent concern among both young and adult patients. The etiology is multifactorial with a genetic background. Prevalence is higher in darker skin types. It has been estimated as high as 30% in a recent Indian study. Females are often more disappointed by POH than males. Treatment has to consider underlying pathologies and patients’ needs. We present our treatment algorithm for POH. In this study, 74 patients with POH, 64 females (86.5%) and 10 males (13.3%), were treated. Of these, 39 patients (53%) had a family history of POH. The age range of patients was 18−57 years (average: 36.1 years). In case of tear trough deformity, soft tissue augmentation was used by injection of hyaluronic acid gel, calcium hydroxylapatite, or autologous fat. Blepharoplasty with partial fat pad resection or repositioning via arcus marginalis release was used to correct severe orbital fat herniation and excess of the lower lid skin. Melanin hyperpigmentation of the skin was improved by sessions of Q-switched 1064 and 532 nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser. Small vessels (capillaries and veins) were targeted by a 1064 nm long-pulsed Nd-YAG laser. Sessions of intense pulsed light (IPL) or CO2 fractional laser were employed to improve skin texture and fine lines. Topical hyaluronic acid-based formulations may be used as adjuvant self-treatment by patients. For pigmented and mixed-type POH, ultraviolet light protection is recommended as a maintenance treatment. By the use of various technologies, treatment can be individually tailored.
Phylogeny of Catabacter hongkongensis Strains Responsible for Bacteremia Is Not Associated with Clinical Outcomes or Therapeutic Efficacy
Authors: Cabrol Matthieu , Rammaert Blandine , Plouzeau Chloé , Burucoa Christophe , Pichon Maxime
Published: March 2021

Catabacter hongkongensis is a Gram-positive rod, isolated in 2007 in blood culture. Fewer than 15 infections have been reported. Herein, we present a lethal case of bacteremia due to C. hongkongensis identified through phylogenetic analyses. A woman was found unconscious in a context of chronic diarrhea. An abdominal abscess with a hydroaeric level was discovered, associated with sigmoid adenocarcinoma and peritoneal carcinomatosis. Despite hospitalization in an ICU and the adaptation of antibiotic therapy, the patient died. Blood cultures were positive in the final stage of the disease (>60 h). Identification of C. hongkongensis was performed using 16S rDNA sequencing. Phylogenetic analyses did not enable classification of these strains according to clinical outcome or the antibiotic sensitivity to treatment. In this case, bacteria were difficult to isolate and MALDI-TOF remained non-contributive. As strains are resistant to probabilistic treatments, addition of metronidazole or vancomycin could optimize clinical management, highlighting the benefit of rapid molecular identification by sequencing.
Multiscale Free Energy Analysis of Human Ecosystem Engineering
Authors: Fox Stephen
Published: March 2021

Unlike ecosystem engineering by other living things, which brings a relatively limited range of sensations that are connected to a few enduring survival preferences, human ecosystem engineering brings an increasing variety and frequency of novel sensations. Many of these novel sensations can quickly become preferences as they indicate that human life will be less strenuous and more stimulating. Furthermore, they can soon become addictive. By contrast, unwanted surprise from these novel sensations may become apparent decades later. This recognition can come after the survival of millions of humans and other species has been undermined. In this paper, it is explained that, while multiscale free energy provides a useful hypothesis for framing human ecosystem engineering, disconnects between preferences and survival from human ecosystem engineering limit the application of current assumptions that underlie continuous state-space and discrete state-space modelling of active inference.
Electrodeposition of Aluminum in the 1-Ethyl-3-Methylimidazolium Tetrachloroaluminate Ionic Liquid
Authors: Shi Meng , Jiang Junhua , Zhao Haiyan
Published: March 2021

The electrodeposition of Al was investigated in an ionic liquid (IL), with 1-ethyl-3-methylimidazolium tetrachloroaluminate ([EMIm]AlCl4) as the electrolyte with AlCl3 precursor. The [EMIm]AlCl4 electrolyte exhibited a wide and stable electrochemical window from 3.2 to 2.3 V on a glassy carbon electrode when temperature was increased from 30 °C to 110 °C. The addition of AlCl3 into [EMIm]AlCl4 generated significant well-developed nucleation growth loops, and new coupled reduction and oxidation peaks in cyclic voltammograms corresponding to the Al deposition and dissolution, respectively. A calculation model was proposed predicting compositions of anions in AlCl3/[EMIm]AlCl4 system, and [Al2Cl7]− was found to be the active species for Al deposition. In AlCl3/[EMIm]AlCl4 (1:5), the reduction rate constants were 1.18 × 10−5 cm s−1 and 3.37 × 10−4 cm s−1 at 30 °C and 110 °C, respectively. Scanning electron microscope (SEM), energy dispersive spectroscope (EDS), and X-ray diffraction (XRD) microscope results showed that the metallic Al film had been successfully deposited on glassy carbon electrodes through constant-potential cathodic reductions. The [EMIm]AlCl4 was a promising electrolyte directly used for Al deposition.
Two-Level-Composite-Hashing Facilitating Highly Efficient Anonymous IoT and D2D Authentication
Authors: Chien Hung-Yu
Published: March 2021

Resource limitation is quite popular in many Internet of Things (IoT) devices and eavesdropping on the identities of IoT devices could reveal the sensitive information; therefore, high efficiency (computation and communication) and anonymity protection are two desirable properties in IoT authentication and in device-to-device (D2D) authentication. Conventionally, dynamic pseudonyms are widely adopted to protect the device identity privacy in IoT authentication and in D2D communications; however, the conventional mechanisms of pseudonym-renewing and pseudonym-bound-public-keys updating could be very costly or be vulnerable to the desynchronization-based denial-of-service (DoS) attacks. In this paper, we propose a novel 2-level composite hashing (2LCH) mechanism to mitigate the problems, and propose the 2LCH-based anonymous IoT and D2D authentication schemes. The schemes simultaneously achieve high efficiency and strong anonymity for such environments; once two devices successfully complete one instance of the server-assist anonymous authentication, they can run several instances of the direct D2D anonymous authentication without the involvement of the server. The merits of the schemes include: (1) high efficiency in terms of computation and communication; (2) easy and efficient generation/synchronization of dynamic pseudonyms; (3) robustness to both desynchronization-based DoS attacks and the unreliable connections; (4) easy application to the existent IoT architectures and standards; and (5) formal security verification.
Area Optimization Techniques for High-Density Spin-Orbit Torque MRAMs
Authors: Seo Yeongkyo , Kwon Kon-Woo
Published: March 2021

This paper presents area optimization techniques for high-density spin-orbit torque magnetic random-access memories (SOT-MRAMs). Although SOT-MRAM has many desirable features of nonvolatility, high reliability and low write energy, it poses challenges to high-density memory implementation because of the use of two access transistors per cell. We first analyze the layout of the conventional SOT-MRAM bit-cell that includes two vertical metal lines, a bit-line and a source-line, limiting the horizontal dimension. We further propose two design techniques to reduce the horizontal dimension by decreasing the number of metal lines per cell without any performance overhead. Based on the fact that adjacent columns in a bit-interleaved array are not simultaneously accessed, the proposed techniques share a single source-line between two consecutive bit-cells in the same row. The simulation result shows that proposed techniques can achieve a bit-cell area reduction of 10–25% compared to the conventional SOT-MRAM. The comparison of our proposed designs with the standard spin-transfer torque MRAM shows 45% lower write energy, 84% lower read energy, and 2.3 × higher read-disturb margin.
Evaluation of Wave Energy Location by Using an Integrated MCDM Approach
Authors: Wang Chia-Nan , Chen Yih-Tzoo , Tung Chun-Chun
Published: March 2021

In recent years, sudden global energy demand has led to the gradual exhaustion of fossil fuel, the world’s main energy resource. With the negative impact of fossil fuel on the environment, governments and organizations have increased R&D funding on renewable energy resources such as solar and wave energy. Vietnam has a great potential for developing wave energy projects owing to the presence of a long coastline and vast ocean. Choosing an optimal location for wave-based power plant projects is a multicriteria decision that requires understanding the quantitative and qualitative elements for assessing the balance of factors when trying to reach the most accurate result. This study proposes a multi-criteria decision-making (MCDM) model, fuzzy-analytic hierarchical process (FAHP), and weighted aggregated sum product assessment (WASPAS) in evaluating potential wave energy stations at the Vietnamese coastline. The authors identify all criteria and sub-criteria affecting the wave power plant location selection process through literature review and expert interview. Selection criteria include wave height, the distance between two waves, number of waves, wind speed, wind duration, ocean depth, turbulence, water quality, coastal erosion, shipping density, protection laws, labor resources, safety conditions, and other related factors. FAHP was used to determining the weights of the identified criteria in the first stage of this study. Finally, the WASPAS model was employed to rank all the alternatives involved in making an effective decision. This study aimed to develop a tool to enhance decision-making when solving fuzzy multi-criteria problems. We propose a real-world model for the effectiveness of the proposed model.
Evaluation of Semi-Solid-State Fermentation of Elaeocarpus serratus L. Leaves and Black Soymilk by Lactobacillus plantarum on Bioactive Compounds and Antioxidant Capacity
Authors: Tsui Chia-Yu , Yang Chun-Yao
Published: March 2021

Elaeocarpus serratus L. leaves (EL) containing phenolic compounds and flavonoids, including myricitrin with pharmacological properties, could be valorized as nutritional additive in foods. In this study, the semi-solid-state fermentation of EL and black soymilk (BS) by Lactobacillus plantarum BCRC 10357 was investigated. Without adding EL in MRS medium, the β-glucosidase activity of L. plantarum quickly reduced to 2.33 ± 0.15 U/mL in 36 h of fermentation; by using 3% EL, the stability period of β-glucosidase activity was prolonged as 12.94 ± 0.69 U/mL in 12 h to 13.71 ± 0.94 in 36 h, showing positive response of the bacteria encountering EL. Using L. plantarum to ferment BS with 3% EL, the β-glucosidase activity increased to 23.78 ± 1.34 U/mL in 24 h, and in the fermented product extract (FPE), the content of myricitrin (2297.06 μg/g-FPE) and isoflavone aglycones (daidzein and genistein, 474.47 μg/g-FPE) at 48 h of fermentation were 1.61-fold and 1.95-fold of that before fermentation (at 0 h), respectively. Total flavonoid content, myricitrin, and ferric reducing antioxidant power in FPE using BS and EL were higher than that using EL alone. This study developed the potential fermented product of black soymilk using EL as a nutritional supplement with probiotics.
Fabrication of Oil-in-Water Emulsions with Whey Protein Isolate–Puerarin Composites: Environmental Stability and Interfacial Behavior
Authors: Zhong Yejun , Zhao Jincheng , Dai Taotao , Ye Jiangping , Wu Jianyong , Chen Tingting , Liu Chengmei
Published: March 2021

Protein–polyphenol interactions influence emulsifying properties in both directions. Puerarin (PUE) is an isoflavone that can promote the formation of heat-set gels with whey protein isolate (WPI) through hydrogen bonding. We examined whether PUE improves the emulsifying properties of WPI and the stabilities of the emulsions. We found that forming composites with PUE improves the emulsifying properties of WPI in a concentration-dependent manner. The optimal concentration is 0.5%, which is the highest PUE concentration that can be solubilized in water. The PUE not only decreased the droplet size of the emulsions, but also increased the surface charge by forming composites with the WPI. A 21 day storage test also showed that the maximum PUE concentration improved the emulsion stability the most. A PUE concentration of 0.5% improved the stability of the WPI emulsions against environmental stress, especially thermal treatment. Surface protein loads indicated more protein was adsorbed to the oil droplets, resulting in less interfacial WPI concentration due to an increase in specific surface areas. The use of PUE also decreased the interfacial tension of WPI at the oil–water interface. To conclude, PUE improves the emulsifying activity, storage, and environmental stability of WPI emulsions. This result might be related to the decreased interfacial tension of WPI–PUE composites.
Fermentation of Agri-Food Waste: A Promising Route for the Production of Aroma Compounds
Authors: Hadj Saadoun Jasmine , Bertani Gaia , Levante Alessia , Vezzosi Fabio , Ricci Annalisa , Bernini Valentina , Lazzi Camilla
Published: March 2021

Food waste and byproducts are generated along the entire food processing and storage chain. The large amount of waste deriving from the whole process represents not only a great economic loss but also an important ethical and environmental issue in terms of failure to recycle potentially reusable materials. New, clear strategies are needed to limit the amount of waste produced and, at the same time, promote its enhancement for further conversion and application to different industrial fields. This review gives an overview of the biological approaches used so far to exploit agri-food wastes and byproducts. The application of solid-state fermentation by different microorganisms (fungi, yeasts, bacteria) to produce several value-added products was analyzed, focusing on the exploitation of lactic acid bacteria as workhorses for the production of flavoring compounds.
Unique Metabolic Profiles of Korean Rice According to Polishing Degree, Variety, and Geo-Environmental Factors
Authors: Kang Yujin , Lee Bo Mi , Lee Eun Mi , Kim Chang-Ho , Seo Jeong-Ah , Choi Hyung-Kyoon , Kim Young-Suk , Lee Do Yup
Published: March 2021

The precise determination of the chemical composition in crops is important to identify their nutritional and functional value. The current study performed a systematic delineation of the rice metabolome, an important staple in Asia, to investigate the following: (1) comparative features between brown and white rice; (2) variety-specific composition (Ilpum vs. Odae); and (3) cultivation of region-dependent metabolic content. Global metabolic profiling and data-driven statistics identified the exclusive enrichment of compounds in brown rice compared to white rice. Next, the authors investigated a variety-governed metabolic phenotype among various geo-environmental factors. Odae, the early-ripening cultivar, showed higher contents of most chemicals compared to the late-ripening cultivar, Ilpum. The authors identified regional specificity for cultivation among five areas in Korea which were characterized by polishing degree and cultivar type. Finally, the current study proposes a possible linkage of the region-specific metabolic signatures to soil texture and total rainfall. In addition, we found tryptophan metabolites that implied the potential for microbe–host interactions that may influence crop metabolites.
Changing Accretion Geometry of Seyfert 1 Mrk 335 with NuSTAR: A Comparative Study
Authors: Mondal Santanu , Stalin C. S.
Published: March 2021

We present a detailed spectral study of the narrow-line Seyfert 1 galaxy, Markarian 335, using eight epoch observations made between 2013 and 2020 with the Nuclear Spectroscopic Telescope Array. The source was variable during this period both in spectral flux and flow geometry. We estimated the height of the Compton cloud from the model fitted parameters for the whole observation period. This allowed us to investigate the underlying physical processes that drive the variability in X-rays. Our model fitted mass varies in a narrow range, between (2.44±0.45−3.04±0.56)×107M⊙, however, given the large error bars, it is consistent with being constant and is in agreement with that known from optical reverberation mapping observations. The disk mass accretion rate reached a maximum of 10% of the Eddington rate during June 2013. Our study sheds light on mass outflows from the system and also compares different aspects of accretion with X-ray binaries.
Virtual Geosites as Innovative Tools for Geoheritage Popularization: A Case Study from Eastern Iceland
Authors: Pasquaré Mariotto Federico , Bonali Fabio Luca
Published: March 2021

In this paper, we have adopted a modern, cutting-edge methodology to make geoheritage sites (geosites) available and explorable worldwide, through both immersive and non-immersive virtual reality, particularly suitable also in COVID-19 times. In doing this, we have focused our attention on five different outcroppings, shallow magma bodies in Iceland: such geological objects, although being often underestimated, are, on the contrary, very suitable for geoheritage popularization purposes. These outstanding outcrops have been transformed in virtual outcrops (VOs) through UAV-based photogrammetry 3D modelling, and have been uploaded on a brand-new, dedicated online resource (GeoVires Virtual Reality Lab for Earth Sciences) which is accessible worldwide for Earth Science teaching and communication. As already stressed above, the choice of these Icelandic shallow magma bodies has been suggested by the fact that such geological objects, although extraordinarily challenging both in terms of geotourism and teaching, are seldom the object of attention from the international scientific community. The five VOs are defined here as virtual geosites (VGs) because they are, indeed, geosites that are fully accessible with a smartphone, a tablet, or a PC; moreover, each is provided with a detailed description and notes available during 3D exploration. Our work could represent a model for future, similar efforts aimed at popularizing Earth Sciences and making geoheritage available to a broad public through VGs.
Verification of the Fredlund (2019) Unsaturated Shear Strength Function
Authors: Tran Thi Phuong An , Fredlund Delwyn G.
Published: March 2021

There has been a proliferation of equations proposed to describe the unsaturated shear strength envelope going back to the 1970s. However, there have been limited studies to verify the suitability of one unsaturated shear strength equation over another. Most proposed shear strength equations have attempted to relate the shear strength of an unsaturated soil to some aspect(s) of the soil–water characteristic curve (SWCC). Estimation procedures have generally focused on using that of air-entry value (AEV) as defined by the drying (or desorption) branch of the degree of saturation SWCC (S-SWCC). This paper studies the suitability of using two “anchor points” (or reference points) along the drying S-SWCC to estimate the unsaturated soil shear strength function. The anchor points referred to are the air-entry value (AEV) of the soil and the “residual suction point” of the soil defined in terms of the S-SWCC. Shear strength conditions associated with both so-called anchor points are used as “boundary conditions” that should be satisfied when estimating the shear strength function for unsaturated soils. Past research laboratory measurements published in the research literature are used as part of the verification process for this study.
Serum Levels of Fibroblast Growth Factor 21 Are Positively Associated with Aortic Stiffness in Patients with Type 2 Diabetes Mellitus
Authors: Huang Sin-Yi , Wu Du-An , Tsai Jen-Pi , Hsu Bang-Gee
Published: March 2021

Aortic stiffness (AS), assessed using carotid–femoral pulse wave velocity (cfPWV), is associated with cardiovascular disease in type 2 diabetes mellitus (T2DM). The relationship between serum fibroblast growth factor 21 (FGF-21) and AS in T2DM patients was evaluated. Fasting serum FGF-21 levels of 130 T2DM patients were measured using an enzyme immunoassay kit. A validated tonometry system was used to measure cfPWV (>10 m/s indicated AS). Of these T2DM patients, 34.6% were defined as the AS group. T2DM patients with AS were older; exhibited higher systolic blood pressure, diastolic blood pressure, and body fat mass; higher triglyceride, fasting glucose, glycosylated hemoglobin, and creatinine levels; higher urine albumin-to-creatinine ratios and serum FGF-21 levels; and lower estimated glomerular filtration rates. The FGF-21 level (odds ratio = 1.005, 95% confidence interval: 1.002–1.009, p = 0.002) as well as systolic blood pressure was an independent predictor of AS and positively correlated to cfPWV values (β = 0.369, p < 0.001) in T2DM patients. For T2DM patients, serum FGF-21 level could be a predictor for AS.
Digital Smile Designed Computer-Aided Surgery versus Traditional Workflow in “All on Four” Rehabilitations: A Randomized Clinical Trial with 4-Years Follow-Up
Authors: Cattoni Francesca , Chirico Luca , Merlone Alberto , Manacorda Michele , Vinci Raffaele , Gherlone Enrico Felice
Published: March 2021

The aim of the present study was to evaluate and compare the traditional “All on Four” technique with digital smile designed computer-aided “All on Four” rehabilitation; with a 4-years follow-up. The protocol was applied to a total of 50 patients randomly recruited and divided in two groups. Digital protocol allows for a completely virtual planning of the exact position of the fixtures, which allows one to perform a flapless surgery procedure with great accuracy (mini-invasive surgery) and also it is possible to use virtually planned prostheses realized with Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) (methods for an immediate loading of the implants. After 4 years from the treatments 98% of success were obtained for the group of patients treated with the traditional protocol and 100% for the digital protocol. At each time interval a significant difference in peri-implant crestal bone loss between the two groups was detected; with an average Marginal Bone Loss (MBL) at 4 years of 1.12 ± 0.26 mm in the traditional group and 0.83 ± 0.11 mm in the digital group. Patients belonging to the digital group have judged the immediate loading (92%), digital smile preview (93%), the mock-up test (98%) and guided surgery (94%) as very effective. All patients treated with a digital method reported lower values of during-surgery and post-surgery pain compared to patients rehabilitated using traditional treatment. In conclusion, the totally digital protocol described in the present study represents a valid therapeutic alternative to the traditional “All on Four” protocol for implant-supported rehabilitations of edentulous dental arches.
Effectiveness of Epidemic Preventive Policies and Hospital Strategies in Combating COVID-19 Outbreak in Taiwan
Authors: Tan Ting Wan , Tan Han Ling , Chang Man Na , Lin Wen Shu , Chang Chih Ming
Published: March 2021

(1) Background: The implementation of effective control measures in a timely fashion is crucial to control the epidemic outbreak of COVID-19. In this study, we aimed to analyze the control measures implemented during the COVID-19 outbreak, as well as evaluating the responses and outcomes at different phases for epidemic control in Taiwan. (2) Methods: This case study reviewed responses to COVID-19 and the effectiveness of a range of control measures implemented for epidemic control in Taiwan and assessed all laboratory-confirmed cases between 11 January until 20 December 2020, inclusive of these dates. The confirmation of COVID-19 infection was defined as the positive result of a reverse-transcriptase–polymerase-chain-reaction test taken from a nasopharyngeal swab. Test results were reported by the Taiwan Centers for Disease Control. The incidence rate, mortality rate, and testing rate were compiled, and the risk ratio was provided to gain insights into the effectiveness of prevention measures. (3) Results and Discussion: This study presents retrospective data on the COVID-19 incidence rate in Taiwan, combined with the vital preventive control measures, in a timeline of the early stage of the epidemic that occurred in Taiwan. The implementation of multiple strategy control measures and the assistance of technologies to control the COVID-19 epidemic in Taiwan led to a relatively slower trend in the outbreak compared to the neighboring countries. In Taiwan, 766 confirmed patients were included, comprised of 88.1% imported cases and 7.2% local transmission cases, within the studied period. The incidence rate of COVID-19 in Taiwan during the studied period was 32 per million people, with a mortality rate of 0.3 per million people. Our analysis showed a significantly raised incidence risk ratio in the countries of interest in comparison to Taiwan during the study period; in the range of 1.9 to 947.5. The outbreak was brought under control through epidemic policies and hospital strategies implemented by the Taiwan Government. (4) Conclusion: Taiwan’s preventive strategies resulted in a drastically lower risk for Taiwan nationals of contracting COVID-19 when new pharmaceutical drug or vaccines were not yet available. The preventive strategies employed by Taiwan could serve as a guide and reference for future epidemic control strategies.
Earnings Management of Insolvent Firms and the Prediction of Corporate Defaults via Discretionary Accruals
Authors: Park Sam Bock , Kim Sung-Kyoo , Lee Sangryul
Published: March 2021

Studies on the characteristics of insolvent firms’ earnings management are critical, as the ripple effects of a firm’s opportunistic accounting and insolvency on society can be widespread and significant. This study divides a dataset of unlisted firms into four groups (large firms that have received external audits; small- and medium-sized enterprises (SMEs) that received external audits; SMES that did not receive external audits; private businesses that did not receive external audits) and analyzes whether there are differences in terms of the discretionary accruals between groups. This study also uses discrete time logit regression to determine if the use of discretionary accruals is predictive of whether unlisted firms would become insolvent. This study used several models (a modified Jones model, a Kothari model, and performance matching model by ROA group) to measure discretionary accruals, which was used as a proxy for earnings management. The results of our study showed that, in the one year prior to insolvency, discretionary accruals were largest among non-externally audited private firms, followed by those of non-externally audited SMEs, externally audited SMEs, and externally audited large firms. The discretionary accruals of non-insolvent firms were larger than those of insolvent firms from the period of one year to three years preceding insolvency, and this difference increased as insolvency approached. The discretionary accruals were shown to have the ability to predict whether or not firms would become insolvent in two to three years before the occurrence of insolvency, but they did not support prediction for one year before the occurrence of insolvency. The findings suggest that additional accounting information should be used together to predict insolvency for unlisted firms.
Tick Size and Price Reversal after Order Imbalance
Authors: Sirnes Espen , Dinh Minh Thi Hong
Published: March 2021

It is well known that intraday returns tend to reverse the following intraday period, conditional on excess buying pressure on the bid or ask side. This suggests that liquidity providers “overreact” to order imbalance (OIB) by initially altering quotes so much that a negative autocorrelation is seen in mid-price returns. We investigate under which circumstances this behavior is most common. Specifically, it seems the tick size augments “OIB-reversal”. However, if the tick size is binding for much of the trading day, it has the opposite effect of censoring such reversals. In addition, if market liquidity is high, the reversal becomes more frequent.
Fibrinolysis: A Primordial System Linked to the Immune Response
Authors: Medcalf Robert L. , Keragala Charithani B.
Published: March 2021

The fibrinolytic system provides an essential means to remove fibrin deposits and blood clots. The actual protease responsible for this is plasmin, formed from its precursor, plasminogen. Fibrin is heralded as it most renowned substrate but for many years plasmin has been known to cleave many other substrates, and to also activate other proteolytic systems. Recent clinical studies have shown that the promotion of plasmin can lead to an immunosuppressed phenotype, in part via its ability to modulate cytokine expression. Almost all immune cells harbor at least one of a dozen plasminogen receptors that allows plasmin formation on the cell surface that in turn modulates immune cell behavior. Similarly, a multitude of pathogens can also express their own plasminogen activators, or contain surface proteins that provide binding sites host plasminogen. Plasmin formed under these circumstances also empowers these pathogens to modulate host immune defense mechanisms. Phylogenetic studies have revealed that the plasminogen activating system predates the appearance of fibrin, indicating that plasmin did not evolve as a fibrinolytic protease but perhaps has its roots as an immune modifying protease. While its fibrin removing capacity became apparent in lower vertebrates these primitive under-appreciated immune modifying functions still remain and are now becoming more recognised.
Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions
Authors: de Andrade Melo-Sterza Fabiana , Poehland Ralf
Published: March 2021

Lipids are a potential reservoir of energy for initial embryonic development before activation of the embryonic genome and are involved in plasma membrane biosynthesis. Excessive lipid droplet formation is detrimental to cryotolerance and is related to alterations in mitochondrial function, which likely affects lipid metabolism. Increased lipid accumulation in in vitro produced embryos is a consequence of the stress during in vitro embryonic development process. There are several open questions concerning embryo lipid metabolism and developmental potential. Oocyte maturation and embryo development in vivo and in vitro may vary if the donors are subjected to any type of stress before follicle puncture because crucial changes in oocyte/embryonic metabolism occur in response to stress. However, little is known about lipid metabolism under additional stress (such as heat stress). Therefore, in this review, we aimed to update the information regarding the energy metabolism of oocytes and early bovine embryos exhibiting developmental competence, focusing on lipid metabolic pathways observed under in vivo, in vitro, and stress conditions.
Involvement of Cytokines in the Pathogenesis of Diabetic Macular Edema
Authors: Noma Hidetaka , Yasuda Kanako , Shimura Masahiko
Published: March 2021

Diabetic macular edema (DME) is a critical complication of diabetic retinopathy, a condition that arises from the breakdown of the blood–retinal barrier and the consequent increase in vascular permeability. Over the years, attempts have been made to treat DME by various approaches, including laser photocoagulation, steroid triamcinolone acetonide, and vitrectomy. However, treatment was unsatisfactory until research identified vascular endothelial growth factor (VEGF) as a factor in the pathogenesis of DME. Intraocular anti-VEGF agents show good efficacy in DME. Nevertheless, in some patients the condition recurs or becomes resistant to treatment, suggesting that other factors may be involved. Because inflammation and retinal hypoxia are seen in DME, research has examined the potential role of cytokines and other inflammatory mediators. In this review, we provide an overview of this research and describe feedback mechanisms that may represent a target for novel treatments.
Amyloid Structural Changes Studied by Infrared Microspectroscopy in Bigenic Cellular Models of Alzheimer’s Disease
Authors: Paulus Agnes , Engdahl Anders , Yang Yiyi , Boza-Serrano Antonio , Bachiller Sara , Torres-Garcia Laura , Svanbergsson Alexander , Garcia Megg G. , Gouras Gunnar K. , Li Jia-Yi , Deierborg Tomas , Klementieva Oxana
Published: March 2021

Alzheimer’s disease affects millions of lives worldwide. This terminal disease is characterized by the formation of amyloid aggregates, so-called amyloid oligomers. These oligomers are composed of β-sheet structures, which are believed to be neurotoxic. However, the actual secondary structure that contributes most to neurotoxicity remains unknown. This lack of knowledge is due to the challenging nature of characterizing the secondary structure of amyloids in cells. To overcome this and investigate the molecular changes in proteins directly in cells, we used synchrotron-based infrared microspectroscopy, a label-free and non-destructive technique available for in situ molecular imaging, to detect structural changes in proteins and lipids. Specifically, we evaluated the formation of β-sheet structures in different monogenic and bigenic cellular models of Alzheimer’s disease that we generated for this study. We report on the possibility to discern different amyloid signatures directly in cells using infrared microspectroscopy and demonstrate that bigenic (amyloid-β, α-synuclein) and (amyloid-β, Tau) neuron-like cells display changes in β-sheet load. Altogether, our findings support the notion that different molecular mechanisms of amyloid aggregation, as opposed to a common mechanism, are triggered by the specific cellular environment and, therefore, that various mechanisms lead to the development of Alzheimer’s disease.
Evidence That Regulation of Pri-miRNA/miRNA Expression Is Not a General Rule of miPEPs Function in Humans
Authors: Prel Anne , Dozier Christine , Combier Jean-Philippe , Plaza Serge , Besson Arnaud
Published: March 2021

Some miRNAs are located in RNA precursors (pri-miRNAs) annotated as long non-coding (lncRNAs) due to absence of long open reading frames (ORFs). However, recent studies have shown that some lnc pri-miRNAs encode peptides called miPEPs (miRNA-encoded peptides). Initially discovered in plants, three miPEPs have also been identified in humans. Herein, we found that a dozen human pri-miRNAs potentially encode miPEPs, as revealed by ribosome profiling and proteomic databases survey. So far, the only known function of plant miPEPs is to enhance the transcription of their own pri-miRNAs, thereby increasing the level and activity of their associated miRNAs and downregulating the expression of their target genes. To date, in humans, only miPEP133 was shown to promote a positive autoregulatory loop. We investigated whether other human miPEPs are also involved in regulating the expression of their miRNAs by studying miPEP155, encoded by the lnc MIR155HG, miPEP497, a sORF-encoded peptide within lnc MIR497HG, and miPEP200a, encoded by the pri-miRNA of miR-200a/miR-200b. We show that overexpression of these miPEPs is unable to impact the expression/activity of their own pri-miRNA/miRNAs in humans, indicating that the positive feedback regulation observed with plant miPEPs and human miPEP133 is not a general rule of human miPEP function.
Identification of a Novel Cis-Acting Regulator of HIV-1 Genome Packaging
Authors: Sakuragi Sayuri , Kotani Osamu , Yokoyama Masaru , Shioda Tatsuo , Sato Hironori , Sakuragi Jun-ichi
Published: March 2021

Human immunodeficiency virus type 1 (HIV-1) uptakes homo-dimerized viral RNA genome into its own particle. A cis-acting viral RNA segment responsible for this event, termed packaging signal (psi), is located at the 5′-end of the viral genome. Although the psi segment exhibits nucleotide variation in nature, its effects on the psi function largely remain unknown. Here we show that a psi sequence from an HIV-1 regional variant, subtype D, has a lower packaging ability compared with that from another regional variant, HIV-1 subtype B, despite maintaining similar genome dimerization activities. A series of molecular genetic investigations narrowed down the responsible element of the selective attenuation to the two sequential nucleotides at positions 226 and 227 in the psi segment. Molecular dynamics simulations predicted that the dinucleotide substitution alters structural dynamics, fold, and hydrogen-bond networks primarily of the psi-SL2 element that contains the binding interface of viral nucleocapsid protein for the genome packaging. In contrast, such structural changes were minimal within the SL1 element involved in genome dimerization. These results suggest that the psi 226/227 dinucleotide pair functions as a cis-acting regulator to control the psi structure to selectively tune the efficiency of packaging, but not dimerization of highly variable HIV-1 genomes.
Adsorption of REEs from Aqueous Solution by EDTA-Chitosan Modified with Zeolite Imidazole Framework (ZIF-8)
Authors: Feng Sihan , Du Xiaoyu , Bat-Amgalan Munkhpurev , Zhang Haixin , Miyamoto Naoto , Kano Naoki
Published: March 2021

Chitosan (CS) modified with ethylenediamine tetraacetic acid (EDTA) was further modified with the zeolite imidazole framework (ZIF-8) by in situ growth method and was employed as adsorbent for the removal of rare-earth elements (REEs). The material (EDTA–CS@ZIF-8) and ZIF-8 and CS were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), and nitrogen adsorption/desorption experiments (N2- Brunauer–Emmet–Teller (BET)). The effects of adsorbent dosage, temperature, the pH of the aqueous solution, contact time on the adsorption of REEs (La(III), Eu(III), and Yb(III)) by EDTA–CS@ZIF-8 were studied. Typical adsorption isotherms (Langmuir, Freundlich, and Dubinin–Radushkevich (D-R)) were determined for the adsorption process, and the maximal adsorption capacity was estimated as 256.4 mg g−1 for La(III), 270.3 mg g−1 for Eu(III), and 294.1 mg g−1 for Yb(III). The adsorption kinetics results were consistent with the pseudo-second-order equation, indicating that the adsorption process was mainly chemical adsorption. The influence of competing ions on REE adsorption was also investigated. After multiple cycles of adsorption/desorption behavior, EDTA–CS@ZIF-8 still maintained high adsorption capacity for REEs. As a result, EDTA–CS@ZIF-8 possessed good adsorption properties such as stability and reusability, which have potential application in wastewater treatment.
Feasibility Study on the Role of Personality, Emotion, and Engagement in Socially Assistive Robotics: A Cognitive Assessment Scenario
Authors: Sorrentino Alessandra , Mancioppi Gianmaria , Coviello Luigi , Cavallo Filippo , Fiorini Laura
Published: March 2021

This study aims to investigate the role of several aspects that may influence human–robot interaction in assistive scenarios. Among all, we focused on semi-permanent qualities (i.e., personality and cognitive state) and temporal traits (i.e., emotion and engagement) of the user profile. To this end, we organized an experimental session with 11 elderly users who performed a cognitive assessment with the non-humanoid ASTRO robot. ASTRO robot administered the Mini Mental State Examination test in Wizard of Oz setup. Temporal and long-term qualities of each user profile were assessed by self-report questionnaires and by behavioral features extrapolated by the recorded videos. Results highlighted that the quality of the interaction did not depend on the cognitive state of the participants. On the contrary, the cognitive assessment with the robot significantly reduced the anxiety of the users, by enhancing the trust in the robotic entity. It suggests that the personality and the affect traits of the interacting user have a fundamental influence on the quality of the interaction, also in the socially assistive context.
Broadening the Scope of Polyoxometalates as Artificial Proteases in Surfactant Solutions: Hydrolysis of Ovalbumin by Zr(IV)-Substituted Keggin Complex
Authors: Savić Nada D. , Salazar Marcano David E. , Quanten Thomas , Parac-Vogt Tatjana N.
Published: March 2021

Development of catalysts for the selective hydrolysis of proteins is challenging, yet important for many applications in biotechnology and proteomics. The hydrolysis of hydrophobic proteins is particularly challenging, as due to their poor solubility, the use of surfactants is often required. In this study, the proteolytic potential of catalyst systems based on the Zr(IV)-substituted Keggin polyoxometalate (Et2NH2)10[Zr(PW11O39)2] (Zr-K 1:2) and three different surfactants (ionic SDS (sodium dodecyl sulfate); zwitterionic Zw3-12 (n-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate); and CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate)), which differ in structure and polarity, has been investigated. Hydrolysis of ovalbumin (OVA) was examined in the presence of Zr-K 1:2 and surfactants by sodium dodecyl sulfate poly(acrylamide) gel electrophoresis (SDS-PAGE), which showed the appearance of new polypeptide fragments at lower molecular weight, indicating that selective hydrolysis of OVA took place for all three catalyst systems. The same fragmentation pattern was observed, showing that the selectivity was not affected by surfactants. However, the surfactants influenced the performance of the catalyst. Hence, the interactions of OVA with surfactants and Zr-K 1:2 were investigated using different techniques such as tryptophan fluorescence, Circular Dichroism, and Dynamic Light Scattering. The speciation of the catalyst in surfactant solutions was also followed by 31P Nuclear Magnetic Resonance spectroscopy providing insight into its stability under reaction conditions.
Mutation in the Ciliary Protein C2CD3 Reveals Organ-Specific Mechanisms of Hedgehog Signal Transduction in Avian Embryos
Authors: Brooks Evan C. , Bonatto Paese Christian Louis , Carroll Anne H. , Struve Jaime N. , Nagy Nandor , Brugmann Samantha A.
Published: March 2021

Primary cilia are ubiquitous microtubule-based organelles that serve as signaling hubs for numerous developmental pathways, most notably the Hedgehog (Hh) pathway. Defects in the structure or function of primary cilia result in a class of diseases called ciliopathies. It is well known that primary cilia participate in transducing a Hh signal, and as such ciliopathies frequently present with phenotypes indicative of aberrant Hh function. Interestingly, the exact mechanisms of cilia-dependent Hh signaling transduction are unclear as some ciliopathic animal models simultaneously present with gain-of-Hh phenotypes in one organ system and loss-of-Hh phenotypes in another. To better understand how Hh signaling is perturbed across different tissues in ciliopathic conditions, we examined four distinct Hh-dependent signaling centers in the naturally occurring avian ciliopathic mutant talpid2 (ta2). In addition to the well-known and previously reported limb and craniofacial malformations, we observed dorsal-ventral patterning defects in the neural tube, and a shortened gastrointestinal tract. Molecular analyses for elements of the Hh pathway revealed that the loss of cilia impact transduction of an Hh signal in a tissue-specific manner at variable levels of the pathway. These studies will provide increased knowledge into how impaired ciliogenesis differentially regulates Hh signaling across tissues and will provide potential avenues for future targeted therapeutic treatments.
A Computational Study on Temperature Variations in MRgFUS Treatments Using PRF Thermometry Techniques and Optical Probes
Authors: Militello Carmelo , Rundo Leonardo , Vicari Fabrizio , Agnello Luca , Borasi Giovanni , Vitabile Salvatore , Russo Giorgio
Published: March 2021

Structural and metabolic imaging are fundamental for diagnosis, treatment and follow-up in oncology. Beyond the well-established diagnostic imaging applications, ultrasounds are currently emerging in the clinical practice as a noninvasive technology for therapy. Indeed, the sound waves can be used to increase the temperature inside the target solid tumors, leading to apoptosis or necrosis of neoplastic tissues. The Magnetic resonance-guided focused ultrasound surgery (MRgFUS) technology represents a valid application of this ultrasound property, mainly used in oncology and neurology. In this paper; patient safety during MRgFUS treatments was investigated by a series of experiments in a tissue-mimicking phantom and performing ex vivo skin samples, to promptly identify unwanted temperature rises. The acquired MR images, used to evaluate the temperature in the treated areas, were analyzed to compare classical proton resonance frequency (PRF) shift techniques and referenceless thermometry methods to accurately assess the temperature variations. We exploited radial basis function (RBF) neural networks for referenceless thermometry and compared the results against interferometric optical fiber measurements. The experimental measurements were obtained using a set of interferometric optical fibers aimed at quantifying temperature variations directly in the sonication areas. The temperature increases during the treatment were not accurately detected by MRI-based referenceless thermometry methods, and more sensitive measurement systems, such as optical fibers, would be required. In-depth studies about these aspects are needed to monitor temperature and improve safety during MRgFUS treatments.