bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Deliberate Hypotension on Regional Cerebral Oxygen Saturation During Functional Endoscopic Sinus Surgery: A Randomized Controlled Trial
Authors: Zhang Ling , Yu Yang , Xue Juan , Lei Weiping , Huang Yaqin , Li Yong , Sun Jianliang
Published: September 2021

Background: Deliberate hypotension can reduce bleeding and improve visualization of the surgical field during functional endoscopic sinus surgery (FESS). However, hypotension may cause brain hypoperfusion and subsequent ischemic injuries, such as delayed awakening, stroke, postoperative delirium, and postoperative cognitive dysfunction. Near-infrared spectroscopy (NIRS) can be used to monitor real-time regional cerebral oxygen saturation (rSO2) levels to estimate brain perfusion. The present study aimed to evaluate the change in rSO2 induced by deliberate hypotension during FESS, and assess the impact of deliberate hypotension on the surgical process.

Material and Methods: A randomized controlled trial was registered with the Chinese clinical trial registry (ChiCTR2000039846). A total of 40 patients were enrolled and randomly divided into the control and intervention groups, and finally, 39 patients were analyzed. Deliberate hypotension was induced in the intervention group using nicardipine and esmolol, whereas the control group received general anesthesia without deliberate hypotension. We recorded mean arterial pressure (MAP), saturation of pulse oximetry (SpO2), rSO2, and heart rate (HR) before induction of anesthesia (T0), immediately after induction of anesthesia (T1), at the beginning of the operation (corresponding with the establishment of deliberate hypotension) (T2), 10 min (T3) and 20 min (T4) after the operation began, at the end of the operation (corresponding with the end of deliberate hypotension) (T5), and 5 min (T6) and 15 min (T7) after the operation. The partial pressure of end-tidal carbon dioxide (PetCO2) was recorded at T1, T2, T3, T4, T5, and T6. The duration of surgery, intraoperative blood loss, tracheal extubation time, and the number of patients that experienced cerebral desaturation events (CDEs) were recorded. The surgical field was estimated postoperation based on the Fromme score.

Results: A 30% decrease from the baseline MAP resulted in a decrease of intraoperative bleeding, improvement in the quality of the surgical field, and the shortening of the duration of surgery during FESS in the intervention group compared with the control group. In addition, rSO2 was reduced and no CDEs were experienced in the intervention group. Linear regression analysis demonstrated a correlation between the decline in rSO2 and that in MAP.

Conclusions: A decrease in MAP to a certain level will cause a decrease of rSO2 in patients undergoing FESS under general anesthesia. Based on our findings, we recommend that the deliberate hypotensive target indicated by MAP be reduced by 30%, while PetCO2 is maintained at 35–40 mmHg and HR is maintained at about 60 beats per minute during FESS.
Biomechanical Evaluation of the Transcortical and Transpedicular Trajectories for Pedicle Screw Insertion in Thoracolumbar Fracture Fixation for Ankylosing Spondylitis
Authors: Tong Zhizhong , Xiao Bin , Yan Kai , Xing Yonggang , Zhang Yanbin
Published: September 2021

Background: Ankylosing spondylitis (AS) is a chronic disorder characterized by an imbalance between bone formation and resorption. Spinal fractures often occur after minor trauma in patients with AS. For thoracolumbar fractures, transpedicular screw (TPS) fixation through the posterior approach has been suggested. The cortical bone trajectory (CBT) technique has also been used to prevent screw pull-out in patients with poor bone quality. The aim of current study was to assess the biomechanical characteristics of the TPS and CBT technique in thoracolumbar AS fracture fixation by finite element analysis.

Methods: The three-dimensional finite element models of the AS spine were created. The CBT and TPS methods of screw insertion were used in AS spinal fracture models. An intact AS spine model was considered the control. An axial force and torsion in rotation, flexion/extension and lateral flexion were applied in all models in CBT, TPS, and control groups.

Results: The AS spine showed similar construct stiffness after posterior fixation by CBT and TPS techniques under axial, rotational, and flexion/extension loading conditions. The TPS technique showed better intact stability under all loading conditions. Similarly, the TPS technique provided superior fracture regional stability against axial and rotational loads than did the CBT technique. The maximum von Mises stresses were 1714.4 ± 129.8 MPa and 1208.7 ± 107.3 MPa (p < 0.001), which occurred in the CBT and TPS groups under compressive loading.

Conclusions: The TPS technique provides better biomechanical strength under axial, rotational, flexion/extension, and lateral flexion loading than does the CBT technique. Compared with CBT, TPS is more effective in maintaining the stability of AS thoracolumbar fractures from a finite element analysis perspective.
Supply Chain and Delivery of Antimicrobial Drugs in Smallholder Livestock Production Systems in Uganda
Authors: Dione Michel Mainack , Amia Winfred Christine , Ejobi Francis , Ouma Emily Awuor , Wieland Barbara
Published: September 2021

This study assessed the veterinary drug supply chain in Uganda, the constraints faced by the actors, and how the challenges influence the use of antimicrobial (AMs) by livestock farmers. We carried out stakeholder consultation workshops, key informant interviews and a knowledge, practices, and awareness survey with actors of the veterinary drug supply chain. We also profiled drugs stored in 23 urban and peri-urban drug shops in Lira and Mukono districts to record the commonly sold drugs. The veterinary drug supply chain is made of several actors including wholesalers, retailers, Animal Health Service Providers (AHSP) and farmers. Nearly ninety per cent of drug retailers and veterinary practitioners did not receive specialized training in veterinary medicine, and most of veterinary practitioners have been in the drug business market for more than 10 years. Antibiotics and anti-helminthics were the most stocked drugs by retailers, with antibiotics ranking highest in terms of contribution to annual financial profits, accounting for 33%. The choice of a drug by veterinary practitioners was mainly informed by past success with efficacy of the drug, and financial capacity of the client (the farmer) to meet the treatment cost. Many veterinary practitioners were not conversant with veterinary drug policies of the country, with Mukono having a higher number (72%) compared to Lira (37%). Veterinary practitioners from Lira district compared to Mukono and those mainly serving small scale farmers relative to large scale smallholders were more knowledgeable about antibiotics and AMR. Several supply chain constraints were identified as potential drivers of misuse of antibiotics that could contribute to AMR. These included low level of education of supply chain actors, particularly drug retailers, poor handling of drugs at purchase and administration practices, low enforcement of policies and regulations, and lack of awareness of stakeholders about policies that regulate drug use. Thus, future interventions to reduce misuse of AM drugs in livestock production systems in Uganda such as capacity building, should also target veterinary input suppliers, and deliberately involve a strong policy advocacy component.
Biomechanical Comparison of Tibial Plateau Leveling Osteotomy Performed With a Novel Titanium Alloy Locking Plate Construct vs. an Established Stainless-Steel Locking Plate Construct
Authors: Bleakley Seth , Palmer Ross , Miller Nate , McGilvray Kirk , Tepic Slobodan
Published: September 2021

A novel canine tibial plateau leveling osteotomy (TPLO) fixation device was recently developed with design features such as titanium alloy (TA) material, distal monocortical screw fixation, and a point contact undersurface specifically targeted to reduce surgical site infection rates by ensuring tissue perfusion under the plate. The strength of the novel TPLO construct was compared with that of a predicate stainless steel (SS) locking plate construct with bicortical screws in 16 paired cadaveric canine limbs. The mean loads to failure were 716.71 ± 109.50 N (range 455.69–839.69 N) and 629.50 ± 176.83 N (range 272.58–856.18 N) in the TA and SS groups, respectively. The average ratio of the loads to failure of the paired specimens was 1.18 (p = 0.031). No failure of the TA constructs involved the distal fixation with monocortical screws. Substantial mechanical equivalence of this novel TA monocortical/bicortical fixation construct to an established SS bicortical screw fixation construct is demonstrated. Clinical investigation of potential merits of this novel TA, monocortical/bicortical locking screw/plate system is now warranted.
Evaluating Disease Threats to Sustainable Poultry Production in Africa: Newcastle Disease, Infectious Bursal Disease, and Avian Infectious Bronchitis in Commercial Poultry Flocks in Kano and Oyo States, Nigeria
Authors: Ekiri Abel B. , Armson Bryony , Adebowale Kehinde , Endacott Isabella , Galipo Erika , Alafiatayo Ruth , Horton Daniel L. , Ogwuche Adah , Bankole Olorunsola N. , Galal Hussein M. , Maikai Beatty-Viv , Dineva Mariana , Wakawa Aliyu , Mijten Erik , Varga Gabriel , Cook Alasdair J. C.
Published: September 2021

The growth of the poultry industry in Nigeria is constrained by major poultry diseases, despite the implementation of vaccination programs. This study aimed to assess the level of protection against Newcastle disease (ND), infectious bursal disease (IBD), and avian infectious bronchitis (IB) afforded by current vaccination schedules and characterize the circulating virus strains in commercial poultry flocks in Nigeria. A cross-sectional study was conducted on 44 commercial poultry farms in Oyo and Kano states of Nigeria. Serum and tissue samples and data on flock, clinical and vaccination records were collected on each farm. Farms were classified as being protected or not protected against ND, IBD and IB based on a defined criterion. Real-time reverse transcription polymerase chain reaction (rRT-PCR) testing was performed for each target virus on tissue samples and positive samples were sequenced. A total of 15/44 (34.1%), 35/44 (79.5%), and 1/44 (2.3%) farms were considered to be protected against ND, IBD, and IB, respectively, at the time of sampling. NDV RNA was detected on 7/44 (15.9%) farms and sequences obtained from 3/7 farms were characterized as the lentogenic strain. Infectious bursal disease virus (IBDV) RNA was detected on 16/44 (36.4%) farms tested; very virulent (vv) IBDV and non-virulent (nv) IBDV strains were both detected in 3/16 (18.8%) positive samples. Sequences of IBDV isolates were either clustered with a group of genotype 3 virulent IBDV strains or were related to vaccine strains MB and D78 strains. IBV RNA was detected on 36/44 (81.8%) farms, with variant02, Massachusetts, 4/91, and Q1 variants detected. Sequences of IBV isolates were either clustered with the vaccines strains Massachusetts M41 and H120 or were most closely related to the D274-like strains or a clade of sequences reported in Nigeria and Niger in 2006 and 2007. This study revealed that most study farms in Oyo and Kano states did not have adequate protective antibody titers against IBV and NDV and were therefore at risk of field challenge. Infectious bursal disease virus and IBV RNA were detected on farms with a history of vaccination suggesting potential vaccination failure, or that the vaccine strains used mismatch with the circulating strains and are therefore not protective.
A Thickness-Mode High-Frequency Underwater Acoustic Transducer with a Low Sidelobe Level
Authors: Zhao Hui , Li Haisen , Wang Yan , Liu Zhenjun , Bian Jiacong , Chen Jianguo
Published: September 2021

Thickness vibration mode is commonly used for high-frequency transducers. For disc piezoelectric ceramics, there is no ideally pure thickness vibration mode because the coupling between the radial and thickness modes always exists. Furthermore, it also deteriorates the transmission voltage response and directivity of the high-frequency transducer. In this paper, based on the theoretical calculation and finite element simulation method, a new method was proposed, and the related experiment was carried out to convince this idea. Both the simulation analysis and experimental results show that drilling a hole at the center of piezoelectric vibration is a simple but effective method to obtain a pure thickness vibration mode of the disc piezoelectric ceramic, and then improve the transmitting ability and directivity of the high-frequency piezoelectric transducer. The sidelobe level is as low as −21.3 dB.
Stabilization of Unstable Second-Order Delay Plants under PID Control: A Nyquist Curve Analysis
Authors: Sun Li , Ma Dan
Published: September 2021

Time delays arise in various components of control systems, including actuators, sensors, control algorithms, and communication links. If not properly taken into consideration, time delays will degrade the closed-loop performance and may even result in instability. This paper studies the stabilization problem of the second-order delay plants with two unstable real poles. Stabilization conditions under PD and PID control are derived using the Nyquist stability criterion. Algorithms for computing feasible PD and PID parameter regions are proposed. In some special cases, the maximal range of delay for stabilization under PD control is also given.
Spatial Price Dynamics and Asymmetric Price Transmission in Skim Milk Powder International Trade: Evidence from Export Prices for New Zealand and Ireland
Authors: Xue Huidan , Li Chenguang , Wang Liming
Published: September 2021

A fast-changing global landscape highlights the importance of understanding spatial price dynamics in key international markets such as China, especially in the era of COVID-19 pandemic with international food trade and food system experiencing an unprecedented challenge. Nowadays, New Zealand’s dominant position in China’s dairy import market is being challenged by European Union (EU) dairy exporters leading to intensified market competition. Using monthly export data of skim milk powder (SMP), we applied threshold cointegration models along with asymmetric error correction models to examine spatial price dynamics and price transmissions of New Zealand and Ireland in Chinese and global markets. We found that New Zealand’s export prices retain their leadership position in China, Ireland’s export prices are well more aligned with those in international markets. In terms of own-country price transmission, Ireland’s relatively symmetric and swift adjustments were found to contrast with New Zealand’s SMP export prices, which displayed more asymmetric price transmissions.
CRY1 Gene Polymorphism and Racing Performance of Homing Pigeons
Authors: Dybus Andrzej , Kulig Hanna , Yu Yu-Hsiang , Lanckriet Ruben , Proskura Witold , Cheng Yeong-Hsiang
Published: September 2021

Cryptochromes (CRY) are the family of proteins proposed as the putative magnetoreceptor molecules. In birds, among others in pigeons, CRY1 is widely expressed in a retina. Homing pigeons are known for their navigational abilities, and pigeon racing is a popular sport. So, the purpose of this study was to analyze the variability of the nucleotide sequence of the homing pigeon CRY1 gene, spanning the region coding the two amino acids W320 and W374 of Trp-triad, and estimate the relationship between genotypes and the racing performance. Investigations were carried out on 129 pigeons. Analysis of sequencing results indicated the AG to TT change within the seventh intron of CRY1 gene. Genotypes were determined by the forced PCR-RFLP method. The influence of detected polymorphism on the results of racing pigeons in 100–400 km flights was shown. The AG/TT individuals achieved significantly higher (p ≤ 0.05) mean values of ace points (AP) than the AG/AG ones. Regarding the detected nucleotide change localization, the polymorphism may be involved in CRY1 gene expression modulation. The AG to TT change in CRY1 gene may be considered as a potential genetic marker of racing performance in homing pigeons.
Temporal Changes in Association Patterns of Cattle Grazing at Two Stocking Densities in a Central Arizona Rangeland
Authors: Tobin Colin T. , Bailey Derek W. , Stephenson Mitchell B. , Trotter Mark G.
Published: September 2021

Proper grazing management of arid and semi-arid rangelands requires experienced personnel and monitoring. Applications of GPS tracking and sensor technologies could help ranchers identify livestock well-being and grazing management issues so that they can promptly respond. The objective of this case study was to evaluate temporal changes in cattle association patterns using global positioning system (GPS) tracking in pastures with different stocking densities (low stocking density [LSD] = 0.123 animals ha−1, high stocking density [HSD] = 0.417 animals ha−1) at a ranch near Prescott, Arizona. Both pastures contained similar herd sizes (135 and 130 cows, respectively). A total of 32 cows in the HSD herd and 29 cows in the LSD herd were tracked using GPS collars at location fixes of 30 min during a 6-week trial in the summer of 2019. A half-weight index (HWI) value was calculated for each pair of GPS-tracked cattle (i.e., dyads) to determine the proportion of time that cattle were within 75 m and 500 m of each other. Forage mass of both pastures were relatively similar at the beginning of the study and forage utilization increased from 5 to 24% in the HSD pasture and increased from 10 to 20% in the LSD pasture. Cattle in both pastures exhibited relatively low mean association values (HWI < 0.25) at both spatial scales. Near the end of the study, cattle began to disperse likely in search of forages (p < 0.01) and travelled farther (p < 0.01) from water than during earlier periods. Real-time GPS tracking has the potential to remotely detect changes in animal spatial association (e.g., HWI), and identify when cows disperse, likely searching for forage.
Microwave-Assisted, One-Pot Synthesis of Doxycycline under Heterogeneous Catalysis in Water
Authors: Bucciol Fabio , Maffeis Elia , Calcio Gaudino Emanuela , Jicsinszky László , Tagliapietra Silvia , Barge Alessandro , Prandi Cristina , Cravotto Giancarlo
Published: September 2021

The selective synthesis of active pharmaceutical molecules is a challenging issue, particularly when attempting to make the reactions even more sustainable. The present work focuses on the microwave-assisted hydrogenolysis of oxytetracycline to selectively produce α-doxycycline. Although the combination of microwave irradiation and a heterogeneous rhodium catalyst provided good conversions, the selective synthesis of active α-doxycycline was only achieved when an oxytetracycline-cyclodextrin complex was used as the starting material, giving the desired product at 34.0% yield in a one-step reaction under very mild conditions.
Neovascularization in Meniscus and Tendon Pathology as a Potential Mechanism in Regenerative Therapies: Special Reference to Platelet-Rich Plasma Treatment
Authors: Szwedowski Dawid , Jaworski Łukasz , Szwedowska Wioleta , Pękala Przemysław , Gagat Maciej
Published: September 2021

Neovascularization is a complex, multistep process that includes the activation of endothelial cells, degradation of the basement membrane surrounding the blood vessel, formation of tip cells, the sprouting, migration and proliferation of endothelial cells into the interstitial space, and then the generation of space in the matrix to allow for the formation of a new, proper lumen of a newly formed blood vessel. Abundant neovascularization can be found in tendinous tissue obtained from asymptomatic athletes or the meniscus early after the injury. The concept of neovascularization in musculoskeletal system disorders seems to be mainly associated with pain and poor clinical outcomes. On the one hand, this phenomenon allows for tissue regeneration, but on the other, it is present during the degeneration process in connective tissue. Establishing the current concept on neovascularization is also needed. A narrative review of the current literature was conducted using databases including Embase, PubMed and Cochrane. This review aims to investigate the exact role of the neovascularization process in tendon and meniscus lesions and its role as a potential target in clinics, specifically in platelet-rich plasma (PRP) therapy. The stabilization of the neovessels required to achieve the healed tissue, together with the standardization of the PRP injections, can offer an alternative future therapeutic approach for the treatment of tendinopathy and meniscal injuries.
A Randomized Controlled Trail for Comparing LED Color Temperature and Color Rendering Attributes in Different Illuminance Environments for Human-Centric Office Lighting
Authors: Lee Sujung , Yoon Heakyung C.
Published: September 2021

In this study, two experiments were conducted to investigate the effects of the color rendering index (CRI) and correlated color temperature (CCT) of light-emitting diode (LED) lighting on office user acceptance and to explore the proper color attributes for human-centric office lighting. Experiment 1 had four LED lights, with two levels for the CRI (CRI < 80: 79, 76; or CRI ≥ 80: 83, 84) and CCT (3000 K or 6500 K) at 300 lux. In experiment 2, there were four LED lights, with several levels for the CRI (CRI < 80: 78; or CRI ≥ 80: 87, 83) and CCT (3000 K or 6500 K) at 500 lux. Ninety-six participants in experiment 1 and ninety-four participants in experiment 2 performed a reading task. The results in experiment 1 and experiment 2 showed that LEDs with lower CRI values at warm color temperatures were rated as more acceptable than LEDs with higher CRI values at warm color temperatures. However, the positive effect extended to LEDs with higher CRI values at cool temperatures but not to LEDs with lower CRI values at cool temperatures. Therefore, the findings are that LEDs with lower CRI values at warm color temperatures and LEDs with higher CRI values at cool temperatures provide the right level of color attributes for office lighting.
Assessment of IsPETase-Assisted Depolymerization of Terephthalate Aromatic Polyesters and the Effect of the Thioredoxin Fusion Domain
Authors: Wagner-Egea Paula , Tosi Virginia , Wang Ping , Grey Carl , Zhang Baozhong , Linares-Pastén Javier A.
Published: September 2021

Terephthalate polyesters such as poly(ethylene terephthalate) (PET) have been massively produced over the last few decades due to their attractive properties in multiple applications. However, due to their limited biodegradability, they have accumulated in landfills and oceans, posing an environmental threat. Enzymatic recycling technologies are predicted to generate long-term socioeconomic benefits. In the present work, we compared the IsPETase (from Ideonella sakaiensis 201-F6) activity on a series of polyesters, including poly(butylene) terephthalate (PBT), poly(hexamethylene) terephthalate (PHT) and Akestra™, with PET. The IsPETase showed remarkable activity toward PET (39% degradation of the original polyester) that was higher than that toward Akestra™ (0.13%), PBT (0.25%) and PHT (0.13%) after 72 h. Thus, based on experimental data and computational analysis, we report insights into IsPETase activity on a series of terephthalate-based polyesters. Aside from that, the fusion domain (Trx) effect in the production and activity of a recombinant Trx-IsPETase is reported.
Thallium Use, Toxicity, and Detoxification Therapy: An Overview
Authors: Genchi Giuseppe , Carocci Alessia , Lauria Graziantonio , Sinicropi Maria Stefania , Catalano Alessia
Published: September 2021

Thallium (Tl) is released into the environment, where is present at very low levels, from both natural and anthropogenic sources. Tl is considered as one of the most toxic heavy metals; it is a non-essential metal, present in low concentrations in humans. Tl toxicity causes dermatological and gastrointestinal diseases and disorders of the nervous system, and may even result in death. Many isotopes of Tl exist, with different uses. One of the isotopes of this metal (201Tl) is used in cardiovascular scintigraphy and for the diagnosis of malignant tumors such as breast or lung cancer and osteosarcoma bone cancer. Many Tl compounds are tasteless, colorless, and odorless. Due to these characteristics and their high toxicity, they have been used as poisons in suicides and murders for criminal purposes, as well as instances of accidental poisoning. Impaired glutathione metabolism, oxidative stress, and disruption of potassium-regulated homeostasis may play a role in the mechanism of Tl toxicity. Solanum nigrum L. and Callitriche cophocarpa have been suggested as promising agents for the phytoremediation of Tl. In addition, macrocyclic compounds such as crown ethers (18-crown-6) are good candidates to absorb Tl from wastewater. Through this review, we present an update to general information about the uses and toxicity of Tl. Furthermore, the attention is focused on detoxification therapies.
Urban Overheating Impact: A Case Study on Building Energy Performance
Authors: Battista Gabriele , Roncone Marta , de Lieto Vollaro Emanuele
Published: September 2021

It is well known that the construction sector is one of the main sectors responsible for energy consumption in the current global energy scenario. Thus, buildings’ energy software become essential tools for achieving energy savings. Climate and its implications for building energy performance are a critical threat. Hence, the aim of this study is to evaluate the climatic conditions in urban and suburban areas of Rome, estimating the incidence of the Urban Heat Island (UHI) phenomenon. To this end, meteorological data obtained from three different areas (two airports and one inside the city) were examined and compared. Then, TRNSYS software was used to create a simple building, in order to assess the impacts of various climatic situations on building energy performance. The study revealed significant percentage differences both in terms of energy needs for heating, from −20.1% to −24.9% when the reference stations are, respectively, Fiumicino and Ciampino, and for cooling, with a wider range, from +48.7% to +87.5% when the reference stations are Ciampino and Fiumicino. Therefore, the study showed the importance of more accurately selecting sets of climate values to be included in energy simulations.
The Effects of Plyometric Conditioning Exercises on Volleyball Performance with Self-Selected Rest Intervals
Authors: Krzysztofik Michal , Kalinowski Rafal , Filip-Stachnik Aleksandra , Wilk Michal , Zajac Adam
Published: September 2021

Post-activation performance enhancement remains a topic of debate in sport science. The purpose of this study was to examine the effects of lower-body plyometric conditioning activity (CA) with a self-selected intra-complex rest interval on upper and lower-body volleyball specific performance. Eleven resistance-trained female volleyball players participated in the study (age: 20 ± 2 years; body mass: 67.8 ± 4.4 kg; height: 178 ± 6 cm; half back squat one-repetition maximum: 78.6 ± 10.2 kg; experience in resistance training: 5.5 ± 2.1 years and in volleyball training: 10 ± 2.3 years). Each participant performed a plyometric CA followed by two different sport-specific tests: an attack jump and a standing spike attack. The changes in jump height (JH), relative mean power output (MP) and ball velocity (BV) were analyzed before and after the CA with self-selected rest intervals. The applied plyometric CA with self-selected intra-complex rest intervals led to an insignificant decline in JH (p = 0.594; effect size [ES]: −0.27) and MP (p = 0.328; ES: −0.46) obtained during the attack jump as well as a significant decline in BV (p = 0.029; ES: −0.72) during the standing spike attack. This study showed that a plyometric CA with self-selected intra-complex rest intervals failed to elicit localized and non-localized PAPE effect in a group of sub-elite volleyball players.
Pushing Mechatronic Applications to the Limits via Smart Motion Control
Authors: Čech Martin , Beltman Arend-Jan , Ozols Kaspars
Published: September 2021

Modern machines strive to run at limit performance and dependability while their operational area and size are getting restricted. To achieve those objectives, often swift integration of custom-made subsystems is required, either actuators, sensors, electronic, or SW modules. Such a diverse suite of elements needs specific approaches and tools for fast optimization and adjustment following model-based system engineering (MBSE) and digital twinning principles. The large-scale I-MECH project was an industry-driven initiative striving to give a scientific response to those demands. The intermediate results were summarized in the authors’ previous work. The purpose of this paper is to report on final project results, namely specific performance achievements and figures based on measurable KPIs. After a brief description of key technologies, special focus is given to industrial printing technology based on a generic substrate carrier. However, it is shown that similar and consistent methodology can be applicable in many other industrial domains, such as semiconductors, healthcare robotics, machining, packaging, etc. Thus, the main merit of this survey is a holistic approach to motion control design.
Multi-Class Strategies for Joint Building Footprint and Road Detection in Remote Sensing
Authors: Ayala Christian , Aranda Carlos , Galar Mikel
Published: September 2021

Building footprints and road networks are important inputs for a great deal of services. For instance, building maps are useful for urban planning, whereas road maps are essential for disaster response services. Traditionally, building and road maps are manually generated by remote sensing experts or land surveying, occasionally assisted by semi-automatic tools. In the last decade, deep learning-based approaches have demonstrated their capabilities to extract these elements automatically and accurately from remote sensing imagery. The building footprint and road network detection problem can be considered a multi-class semantic segmentation task, that is, a single model performs a pixel-wise classification on multiple classes, optimizing the overall performance. However, depending on the spatial resolution of the imagery used, both classes may coexist within the same pixel, drastically reducing their separability. In this regard, binary decomposition techniques, which have been widely studied in the machine learning literature, are proved useful for addressing multi-class problems. Accordingly, the multi-class problem can be split into multiple binary semantic segmentation sub-problems, specializing different models for each class. Nevertheless, in these cases, an aggregation step is required to obtain the final output labels. Additionally, other novel approaches, such as multi-task learning, may come in handy to further increase the performance of the binary semantic segmentation models. Since there is no certainty as to which strategy should be carried out to accurately tackle a multi-class remote sensing semantic segmentation problem, this paper performs an in-depth study to shed light on the issue. For this purpose, open-access Sentinel-1 and Sentinel-2 imagery (at 10 m) are considered for extracting buildings and roads, making use of the well-known U-Net convolutional neural network. It is worth stressing that building and road classes may coexist within the same pixel when working at such a low spatial resolution, setting a challenging problem scheme. Accordingly, a robust experimental study is developed to assess the benefits of the decomposition strategies and their combination with a multi-task learning scheme. The obtained results demonstrate that decomposing the considered multi-class remote sensing semantic segmentation problem into multiple binary ones using a One-vs.-All binary decomposition technique leads to better results than the standard direct multi-class approach. Additionally, the benefits of using a multi-task learning scheme for pushing the performance of binary segmentation models are also shown.
Precipitation Trends in the Ganges-Brahmaputra-Meghna River Basin, South Asia: Inconsistency in Satellite-Based Products
Authors: Khatiwada Muna , Curtis Scott
Published: September 2021

The Ganges-Brahmaputra-Meghna (GBM) river basin is the world’s third largest. Literature show that changes in precipitation have a significant impact on climate, agriculture, and the environment in the GBM. Two satellite-based precipitation products, Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks-Climate Data Record (PERSIANN-CDR) and Multi-Source Weighted-Ensemble Precipitation (MSWEP), were used to analyze and compare precipitation trends over the GBM as a whole and within 34 pre-defined hydrological sub-basins separately for the period 1983–2019. A non-parametric Modified Mann-Kendall test was applied to determine significant trends in monsoon (June–September) and pre-monsoon (March–May) precipitation. The results show an inconsistency between the two precipitation products. Namely, the MSWEP pre-monsoon precipitation trend has significantly increased (Z-value = 2.236, p = 0.025), and the PERSIANN-CDR monsoon precipitation trend has significantly decreased (Z-value = −33.071, p < 0.000). However, both products strongly indicate that precipitation has recently declined in the pre-monsoon and monsoon seasons in the eastern and southern regions of the GBM river basin, agreeing with several previous studies. Further work is needed to identify the reasons behind inconsistent decreasing and increasing precipitation trends in the GBM river basin.
Emission Lines in 290–360 nm of Highly Charged Tungsten Ions W20+–W29+
Authors: Era Shota , Kato Daiji , Sakaue Hiroyuki A. , Umezaki Toshiki , Nakamura Nobuyuki , Murakami Izumi
Published: September 2021

Forbidden transitions in the near-UV and visible wavelength of highly charged tungsten (W) ions are potentially useful as novel tungsten diagnostics means of fusion plasmas. Emission lines in 290–360 nm from Wq+ ions interacting with an electron beam of 540–1370 eV are measured, using a compact electron-beam-ion-trap. The charge states of 64 lines are identified as W20+–W29+. A magnetic-dipole (M1) line of W29+ between the excited states (4d84f)[(4d5/2−2)44f7/2]13/2→[(4d5/2−2)44f5/2]13/2 is newly identified; the wavelength is determined as 351.03(10) nm in air. The theoretical wavelength calculated using the multiconfiguration Dirac–Hartree–Fock method is in a good agreement with the measurement.
Trends in Nanotechnology and Its Potentialities to Control Plant Pathogenic Fungi: A Review
Authors: Kutawa Abdulaziz Bashir , Ahmad Khairulmazmi , Ali Asgar , Hussein Mohd Zobir , Abdul Wahab Mohd Aswad , Adamu Abdullahi , Ismaila Abubakar A. , Gunasena Mahesh Tiran , Rahman Muhammad Ziaur , Hossain Md Imam
Published: September 2021

Approximately 15–18% of crops losses occur as a result of animal pests, while weeds and microbial diseases cause 34 and 16% losses, respectively. Fungal pathogens cause about 70–80% losses in yield. The present strategies for plant disease control depend transcendently on agrochemicals that cause negative effects on the environment and humans. Nanotechnology can help by reducing the negative impact of the fungicides, such as enhancing the solubility of low water-soluble fungicides, increasing the shelf-life, and reducing toxicity, in a sustainable and eco-friendly manner. Despite many advantages of the utilization of nanoparticles, very few nanoparticle-based products have so far been produced in commercial quantities for agricultural purposes. The shortage of commercial uses may be associated with many factors, for example, a lack of pest crop host systems usage and the insufficient number of field trials. In some areas, nanotechnology has been advanced, and the best way to be in touch with the advances in nanotechnology in agriculture is to understand the major aspect of the research and to address the scientific gaps in order to facilitate the development which can provide a rationale of different nanoproducts in commercial quantity. In this review, we, therefore, described the properties and synthesis of nanoparticles, their utilization for plant pathogenic fungal disease control (either in the form of (a) nanoparticles alone, that act as a protectant or (b) in the form of a nanocarrier for different fungicides), nano-formulations of agro-nanofungicides, Zataria multiflora, and ginger essential oils to control plant pathogenic fungi, as well as the biosafety and limitations of the nanoparticles applications.
Handgrip Strength and Its Relationship with White Blood Cell Count in U.S. Adolescents
Authors: López-Gil José Francisco , Ramírez-Vélez Robinson , Izquierdo Mikel , García-Hermoso Antonio
Published: September 2021

Background: The role of muscular fitness (as a protecting factor for an optimal immune system) and WBC count remains unclear. To the best of our knowledge, this is the first study to investigate the relationship between the total count of WBC and groups based on handgrip strength and body composition. The aim of this study was to elucidate the relationship between handgrip strength and WBC count in a nationally representative sample of adolescents from the U.S. Methods: We used data from the NHANES cross-sectional study (waves 2011 to 2014). The final analysis included 917 adolescents from 12–17 years old (51.0% boys). Normalized handgrip strength (kg) (NHS) was relativized by body composition parameters (body weight [NHSw], total body fat [NHSf], and trunk fat [NHSt]) assessed with dual energy X-ray absorptiometry. Results: An inverse association was found between total WBC count and all assessments of low NHS in both sexes (p < 0.05). Both boys and girls with low NHS had higher WBC counts in all estimations of NHS than those with high NHS (p < 0.05 for all). All estimations of low NHS showed significant differences with those with intermediate NHS (only in girls) (p < 0.05 for all). Girls with intermediate NHSt exhibited higher WBC count than those with high NHSt (p = 0.004). Conclusions: Our findings suggest that greater levels of NHS are linked to lower total WBC counts. From a public health perspective, our findings are clinically significant and emphasize the relevance of improving muscular fitness during adolescence since it may contribute to boosting the immune system among adolescents.
Metabolic Regulation of Sugar Assimilation for Lipid Production in Aspergillus oryzae BCC7051 through Comparative Transcriptome Perspective
Authors: Vorapreeda Tayvich , Khongto Bhimabol , Thammarongtham Chinae , Srisuk Tanawut , Laoteng Kobkul
Published: September 2021

Microbial lipid production with cost effectiveness is a prerequisite for the oleochemical sector. In this work, genome-wide transcriptional responses on the utilization of xylose and glucose in oleaginous Aspergillus oryzae were studied with relation to growth and lipid phenotypic traits. Comparative analysis of the active growth (t1) and lipid-accumulating (t2) stages showed that the C5 cultures efficiently consumed carbon sources for biomass and lipid production comparable to the C6 cultures. By pairwise comparison, 599 and 917 differentially expressed genes (DEGs) were identified in the t1 and t2 groups, respectively, in which the consensus DEGs were categorized into polysaccharide-degrading enzymes, membrane transports, and cellular processes. A discrimination in transcriptional responses of DEGs set was also found in various metabolic genes, mostly in carbohydrate, amino acid, lipid, cofactors, and vitamin metabolisms. Although central carbohydrate metabolism was shared among the C5 and C6 cultures, the metabolic functions in acetyl-CoA and NADPH generation, and biosynthesis of terpenoid backbone, fatty acid, sterol, and amino acids were allocated for leveraging biomass and lipid production through at least transcriptional control. This study revealed robust metabolic networks in the oleaginicity of A. oryzae governing glucose/xylose flux toward lipid biosynthesis that provides meaningful hints for further process developments of microbial lipid production using cellulosic sugar feedstocks.
Warming Decreases Bioconversion of Polyunsaturated Fatty Acids in Chironomid Larvae Maintained on Cyanobacterium Microcystis
Authors: Strandberg Ursula , Ilo Timo , Akkanen Jarkko , Kankaala Paula
Published: September 2021

Cyanobacteria dominance and warming have been suggested to decrease the production of polyunsaturated fatty acids (PUFA) in freshwater ecosystems. Physiological adaptations of poikilothermic animals to higher temperatures may further decrease PUFA levels in aquatic food webs. We conducted diet manipulation experiments to investigate the combined effects of dietary PUFA and warming on the proportions of eicosapentaenoic acid (EPA) and arachidonic acid (ARA) in Chironomus riparius. The experimental diet consisted of a nontoxic cyanobacterium Microcystis, which contained C20 PUFA: 20:3n-3, 20:4n-3, and 20:3n-6, but no EPA or ARA. Additionally, we used TetraMin® fish flakes as a control treatment. A temperature increase from 20 °C to 25 °C decreased the proportion of n-3 C20 PUFA and the n-3/n-6 ratio in Microcystis. Diet manipulation experiments indicated that Chironomus desaturated dietary C20 precursors to EPA and ARA, but warming decreased this bioconversion and resulted in lower levels of EPA and ARA in Chironomus. Warming did not alter the proportions of EPA and ARA in Chironomus larvae if these PUFA were readily available in the diet (TetraMin® control treatment). In conclusion, warming and cyanobacteria dominance may decrease the production and trophic transfer of physiologically important PUFA in freshwaters by (1) decreasing the n-3/n-6 ratio and the abundance of n-3 C20 precursors in Microcystis, and (2) decreasing the bioconversion of n-3 and n-6 C20 precursors to EPA and ARA in chironomids. These changes may have cascading effects throughout the food web and decrease the content of EPA in fish, potentially affecting its availability to humans.
Out of Control: The Role of the Ubiquitin Proteasome System in Skeletal Muscle during Inflammation
Authors: Haberecht-Müller Stefanie , Krüger Elke , Fielitz Jens
Published: September 2021

The majority of critically ill intensive care unit (ICU) patients with severe sepsis develop ICU-acquired weakness (ICUAW) characterized by loss of muscle mass, reduction in myofiber size and decreased muscle strength leading to persisting physical impairment. This phenotype results from a dysregulated protein homeostasis with increased protein degradation and decreased protein synthesis, eventually causing a decrease in muscle structural proteins. The ubiquitin proteasome system (UPS) is the predominant protein-degrading system in muscle that is activated during diverse muscle atrophy conditions, e.g., inflammation. The specificity of UPS-mediated protein degradation is assured by E3 ubiquitin ligases, such as atrogin-1 and MuRF1, which target structural and contractile proteins, proteins involved in energy metabolism and transcription factors for UPS-dependent degradation. Although the regulation of activity and function of E3 ubiquitin ligases in inflammation-induced muscle atrophy is well perceived, the contribution of the proteasome to muscle atrophy during inflammation is still elusive. During inflammation, a shift from standard- to immunoproteasome was described; however, to which extent this contributes to muscle wasting and whether this changes targeting of specific muscular proteins is not well described. This review summarizes the function of the main proinflammatory cytokines and acute phase response proteins and their signaling pathways in inflammation-induced muscle atrophy with a focus on UPS-mediated protein degradation in muscle during sepsis. The regulation and target-specificity of the main E3 ubiquitin ligases in muscle atrophy and their mode of action on myofibrillar proteins will be reported. The function of the standard- and immunoproteasome in inflammation-induced muscle atrophy will be described and the effects of proteasome-inhibitors as treatment strategies will be discussed.
Promising Biomarkers of Radiation-Induced Lung Injury: A Review
Authors: Liu Xinglong , Shao Chunlin , Fu Jiamei
Published: September 2021

Radiation-induced lung injury (RILI) is one of the main dose-limiting side effects in patients with thoracic cancer during radiotherapy. No reliable predictors or accurate risk models are currently available in clinical practice. Severe radiation pneumonitis (RP) or pulmonary fibrosis (PF) will reduce the quality of life, even when the anti-tumor treatment is effective for patients. Thus, precise prediction and early diagnosis of lung toxicity are critical to overcome this longstanding problem. This review summarizes the primary mechanisms and preclinical animal models of RILI reported in recent decades, and analyzes the most promising biomarkers for the early detection of lung complications. In general, ideal integrated models considering individual genetic susceptibility, clinical background parameters, and biological variations are encouraged to be built up, and more prospective investigations are still required to disclose the molecular mechanisms of RILI as well as to discover valuable intervention strategies.
Nivolumab Reduces PD1 Expression and Alters Density and Proliferation of Tumor Infiltrating Immune Cells in a Tissue Slice Culture Model of Renal Cell Carcinoma
Authors: Stenzel Philipp J. , Hörner Nina , Foersch Sebastian , Wagner Daniel-Christoph , Tsaur Igor , Thomas Anita , Haferkamp Axel , Macher-Goeppinger Stephan , Roth Wilfried , Porubsky Stefan , Tagscherer Katrin E.
Published: September 2021

Background: In the treatment of clear cell renal cell carcinoma (ccRCC), nivolumab is an established component of the first-line therapy with a favorable impact on progression free survival and overall survival. However, treatment-related adverse effects occur and, to date, there is no approved predictive biomarker for patient stratification. Thus, the aim of this study was to establish an ex vivo tissue slice culture model of ccRCC and to elucidate the impact of nivolumab on tumor infiltrating immune cells. Methods: Fresh tumor tissue of ccRCC was treated with the immune checkpoint inhibitor nivolumab using ex vivo tissue slice culture (TSC). After cultivation, tissue slices were formalin-fixed, immunohistochemically stained and analyzed via digital image analysis. Results: The TSC model was shown to be suitable for ex vivo pharmacological experiments on intratumoral immune cells in ccRCC. PD1 expression on tumor infiltrating immune cells was dose-dependently reduced after nivolumab treatment (p < 0.01), whereas density and proliferation of tumor infiltrating T-cells and cytotoxic T-cells were inter-individually altered with a remarkable variability. Tumor cell proliferation was not affected by nivolumab. Conclusions: This study could demonstrate nivolumab-dependent effects on PD1 expression and tumor infiltrating T-cells in TSC of ccRCC. This is in line with results from other scientific studies about changes in immune cell proliferation in peripheral blood in response to nivolumab. Thus, TSC of ccRCC could be a further step to personalized medicine in terms of testing the response of individual patients to nivolumab.