bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of environmental factors on reproduction of the invasive Gulf toadfish Opsanus beta (Teleostei: Batrachoididae) in a subtropical estuary
Authors: de Assis Thara Santiago , Nagata Jeferson Keiti , Evans Allan Arnold , Morimoto Juliano , Fávaro Luís Fernando
Published: February 2024

A hallmark of biological invasion is the rapid reproduction of invasive species. However, we still do not have an adequate understanding of how fluctuations in environmental factors affect the reproduction of invasive species, particularly in estuarine habitats. In this study, we evaluated the reproduction of the Gulf toadfish Opsanus beta and its relationships with temperature and rainfall in the Paranaguá estuary, a subtropical region in southern Brazil. This species was likely introduced into the Brazilian ecosystems through the discharge of ballast water. Since invasion, which was first reported in port areas, the species has rapidly spread along the Brazilian coast line. We collected specimens monthly from October 2011 to September 2012 and from October 2019 to September 2020, to give us insight into the seasonal and temporal changes in the reproduction of this species. We found that O. beta population uses the estuary throughout its life cycle, including during spawning. Females and males spawned throughout almost the entire sampling period and spawning was specified as partially. Peak reproductive activity occurred in periods of lower temperature and was also related to rain. The length of first maturation in females (10cm) was considered early, indicating reproductive activity in smaller individuals. Our results demonstrate that the reproductive strategy used by the invasive species supports its ability to successfully colonize new environments.
Divine Music in Archaic and Classical Greek Art
Authors: Laferrière Carolyn
Published: February 2024

In this volume, Carolyn M. Laferrière examines Athenian vase-paintings and reliefs depicting the gods most frequently shown as musicians to reconstruct how images suggest the sounds of the music the gods made. Incorporating insights from recent work in sensory studies, she applies formal analysis together with literary and archaeological evidence to reconstruct the musical culture of Athens. Laferrière shows how images suggest the sounds of the gods' music. This representational strategy, whereby sight and sound are blurred, conveys the 'unhearable' nature of their music: Because it cannot be physically heard, it falls to human imagination to provide its sounds and awaken viewers' multisensory engagement. Moreover, when situated within their likely original contexts, the objects establish a network of interaction between the viewer, the visualized music, and the landscape, all of which determined how divine music was depicted, perceived, and reciprocated. Laferrière demonstrates that participation in the gods' musical performances offered worshippers an multisensory experience of divine presence.
Diversifying the research landscape: Assessing barriers to research for underrepresented populations in an online study of Parkinson’s disease
Authors: Sanchez Angie V. , Ison Juliana M. , Hemley Helen , Jackson Jonathan D.
Published: February 2024

Despite federal regulations mandating the inclusion of underrepresented groups in research, recruiting diverse participants remains challenging. Identifying and implementing solutions to recruitment barriers in real time might increase the participation of underrepresented groups. Hence, the present study created a comprehensive dashboard of barriers to research participation. Barriers to participation were recorded in real time for prospective participants. Overall, 230 prospective participants expressed interest in the study but were unable to join due to one or more barriers. Awareness of the most common obstacles to research in real time will give researchers valuable data to meaningfully modify recruitment methods.
Surface gravity wave-induced drift of floating objects in the diffraction regime
Authors: Xiao Q. , Calvert R. , Yan S.Q. , Adcock T.A.A. , van den Bremer T.S.
Published: February 2024

Floating objects will drift due to the action of surface gravity waves. This drift will depart from that of a perfect Lagrangian tracer due to both viscous effects (non-potential flow) and wave–body interaction (potential flow). We examine the drift of freely floating objects in regular (non-breaking) deep-water wave fields for object sizes that are large enough to cause significant diffraction. Systematic numerical simulations are performed using a hybrid numerical solver, qaleFOAM, which deals with both viscosity and wave–body interaction. For very small objects, the model predicts a wave-induced drift equal to the Stokes drift. For larger objects, the drift is generally greater and increases with object size (we examine object sizes up to$10\,\%$of the wavelength). The effects of different shapes, sizes and submergence depths and steepnesses are examined. Furthermore, we derive a ‘diffraction-modified Stokes drift’ akin to Stokes (Trans. Camb. Phil. Soc., vol. 8, 1847, pp. 411–455), but based on the combination of incident, diffracted and radiated wave fields, which are based on potential-flow theory and obtained using the boundary element method. This diffraction-modified Stokes drift explains both qualitatively and quantitatively the increase in drift. Generally, round objects do not diffract the wave field significantly and do not experience a significant drift enhancement as a result. For box-shape objects, drift enhancement is greater for larger objects with greater submergence depths (we report an increase of$92\,\%$for simulations without viscosity and$113\,\%$with viscosity for a round-cornered box whose size is$10\,\%$of the wavelength). We identify the specific standing wave pattern that arises near the object because of diffraction as the main cause of the enhanced drift. Viscosity plays a small positive role in the enhanced drift behaviour of large objects, increasing the drift further by approximately$20\,\%$.
Critical Reflections on the Role of the Canon in New Testament Scholarship
Authors: Watson Francis
Published: February 2024

The modern discipline of New Testament Studies has subjected the various components of the New Testament to close scrutiny, yet it persistently fails to ask critical questions about the New Testament considered as a whole. In its familiar twenty-seven book form, the New Testament may be seen as a fourth-century anthology of early Christian writings based on earlier collections or sub-collections (the fourfold gospel, the Pauline letter collection), yet innovative in establishing a sharply defined boundary between included and excluded texts. An analysis of contributions to this journal over a recent five-year period demonstrates the pervasive influence of this fourth-century construct in determining the scope and priorities of (so-called) ‘mainstream’ scholarship. Greater attention to the contingencies of canon-formation will enable us to locate the texts that came to form the New Testament within a wider early Christian literary landscape.
An Extended Inverted Allusion to Psalm 22 in Mark 15: Reading Reversal in the Markan Passion
Authors: Combs Jason Robert
Published: February 2024

The Markan Passion narrative alludes to Ps 22 (LXX Ps 21) in reverse, culminating with Jesus’ cry: ‘My God, my God, why have you forsaken me?’ (Mark 15.34; cf. Ps 22.1). I argue that this ‘extended inverted allusion’ was an admired literary technique. Through select examples of this technique in the writings of the Hebrew Bible and Greco-Roman literature, I demonstrate its various functions—it can be employed to reverse meaning, to dissociate causation or to create new narrative trajectories. Reading Mark 15 in light of the literary functions of inverted allusion reveals new interpretive possibilities. In the Septuagint, Psalm 21 suggests that the psalmist's suffering was merited because of transgressions, but the inverted allusions to this Psalm in Mark 15 reinforce that Jesus’ suffering is unmerited (cf. Mark 15.10, 14) by decoupling the suffering from the transgressions. Additionally, in LXX Ps 21, the psalmist moves from forsakenness on account of transgressions toward divine deliverance. By alluding to this Psalm in reverse, Jesus travels the psalmist's journey in reverse. Rather than move from forsakenness toward divine deliverance, Mark's Jesus moves toward forsakenness, precisely to bring about divine deliverance.
Risk Factors Associated with Missing Incidents among Persons Living with Dementia: A Scoping Review
Authors: Perez Hector , Miguel Cruz Antonio , Neubauer Noelannah , Daum Christine , Comeau Aidan K. , Marshall Samantha Dawn , Letts Elyse , Liu Lili
Published: February 2024

Worldwide, over 55-million people have dementia, and the number will triple by 2050. Persons living with dementia are exposed to risks secondary to cognitive challenges including getting lost. The adverse outcomes of going missing include injuries, death, and premature institutionalization. In this scoping review, we investigate risk factors associated with going missing among persons living with dementia. We searched and screened studies from four electronic databases (Medline, CINAHL, Embase, and Scopus), and extracted relevant data. We identified 3,376 articles, of which 73 met the inclusion criteria. Most studies used quantitative research methods. We identified 27 variables grouped into three risk factor domains: (a) demographics and personal characteristics, (b) health conditions and symptoms, and (c) environmental and contextual antecedents. Identification of risk factors associated with getting lost helps to anticipate missing incidents. Risk factors can be paired with proactive strategies to prevent incidents and inform policies to create safer communities.
Modeling and optimization of a relativistic magnetron with transparent cathode and output mode
Authors: Sawert David , Vallejos Pablo , Nyberg Frans , Hurtig Tomas
Published: February 2024

This paper outlines the results of particle-in-cell simulations of a relativistic magnetron with six cavities and a transparent cathode configuration. Excitation of the π mode in the interaction region was attained, which in turn led to

$\textrm{TE}_{11}$

mode emission of microwaves to the waveguide. This mode transformation was achieved with a non-symmetric diffraction output, consisting of four large and two small tapered cavities. Simulations were performed with a voltage across the anode-cathode gap varying from 164 to 356 kV, and axial magnetic field strengths between 0.24 and 0.34 T. Maximum efficiency of 37% was obtained with a peak output power of 590 MW, having a voltage of 261 kV and a magnetic field of 0.30 T. Furthermore, a frequency of 2.57 GHz and a rise time of microwaves at the waveguide of 15 ns were demonstrated. The electron leakage current was shown to decrease from ∼10

$\%$

to less than

$1\%$

when employing a longer interaction region, while still exhibiting good performance. Additionally, we show that there is an optimal range of voltages given a magnetic field, for which π mode excitation with high efficiency is attained.
Towards integration? Considering social aspects with large-scale computational models for nature-based solutions
Authors: Schulte Ingrid , Yowargana Ping , Nielsen Jonas Ø. , Kraxner Florian , Fuss Sabine
Published: February 2024

Non-Technical Summary

Information on social aspects of climate change intervention, such as behavioral choices and public acceptance, are often not included in global climate models. As a result, they have been critiqued for not adequately reflecting ‘real world’ conditions. At the same time, these models are important and influential policy tools. To improve these models, calls are being made for more interaction – or integration – between the social science and modelling research communities. Yet, it remains unclear how to achieve this. Responding to this gap, we explore what kind of integration is currently taking place, how, and opportunities for further development.

Technical Summary

The importance of social drivers of climate change interventions, or social aspects, is currently underrepresented in computational modelling projections. These parameters are largely excluded from estimates of technical mitigation potential, feasibility, and tools like integrated assessment models (IAMs) and other large-scale models that influence the development of climate policies and notable bodies like the Intergovernmental Panel on Climate Change. This paper contributes to calls being made within the research community to address this gap and strengthen linkages between modelling practices and social science insights. Using nature-based solutions (NbS) as a framing, we present the results of a critical literature review and interviews with multidisciplinary experts reflecting on the current state of integration around IAMs and opportunities to better capture social aspects within large-scale modelling processes. Our findings confirm the need to incorporate social aspects in IAMs, but highlight that how this happens in practice may depend on context, project objectives, or pragmatic choices rather than conceptual notions about what ‘good’ integration is. Nevertheless, some integration strategies are better than others, and concerns about data limitations and low capacity of the IAM community for engaging in integration can be overcome with sufficient support and complementary efforts from the broader research community.

Social Media Summary

Integrating social aspects in large-scale models requires complementary efforts from the broader research community.
Zusammenarbeit von Pflegepädagog_innen und Praxisanleiter_innen
Authors: Stöger Andreas
Published: February 2024

Zusammenfassung: Die zukünftigen demographischen Entwicklungen betreffen Pflegepädagog_innen in besonderem Maße, die für genügend Arbeitskräfte sorgen sollen. Umso wichtiger erscheint die Qualität kollegialer Zusammenarbeit mit Praxisanleiter_innen. In dieser Masterarbeit wird die Inhaltsanalyse nach Glaser und Laudel durchgeführt. Weiter wird die Konzipierung eines Workshops vorgenommen und kritisch betrachtet.
Mixing of Rain and River Water in the Bay of Bengal From Basin‐Scale Freshwater Balance
Authors: Jarugula Sreelekha , Sengupta Debasis , Shroyer Emily , Papa Fabrice
Published: February 2024

We construct freshwater balance in the Bay of Bengal (BoB) within a control volume (CV) bounded by 1,018 kg/m3 isopycnal surface using observations and ocean reanalysis during 2011–2015. Freshwater in CV is maximum in October–November due to monsoonal rain and river inflows, and minimum in April–May. Water lighter than 1,018 kg/m3 is not transported out of BoB, implying that freshwater lost from CV is mixed away entirely within the basin. From freshwater budget, we infer moderate diapycnal mixing rates (∼0.8 × 10−5 m2/s) in boreal spring and summer; in winter (December–January), the rate of freshwater loss to subsurface ocean is 0.015 m/day, corresponding to a median turbulent diffusivity of 4.2 × 10−5 m2/s, with standard error of 25%. We show that enhanced winter mixing across the shallow pycnocline is due to reduced shortwave radiation and subseasonal episodes of surface buoyancy loss when cool, dry northeast monsoon winds blow over BoB.
Climatic Drivers of Ice Slabs and Firn Aquifers in Greenland
Authors: Brils M. , Munneke P. Kuipers , Jullien N. , Tedstone A. J. , Machguth H. , van de Berg W. J. , van den Broeke M. R.
Published: February 2024

Recent observations revealed the existence of ice slabs and aquifers on the Greenland ice sheet (GrIS). Both affect the ice sheet's hydrology: ice slabs facilitate runoff and aquifers modulate drainage to the bed. However, their climatic drivers and history remain unclear, as most observations cover only two decades. Here, we present a model simulation of the evolution of GrIS ice slabs and aquifers (1980–2020), evaluated using radar measurements. The results show that accumulation, melt and rain rates are good predictors for the spatial distribution of ice slabs and aquifers. Both features were already present in the late 1980s, and their extent remained relatively constant until the beginning of this century, after which increased melt led to their expansion. We show that almost any transect from the coast to the ice‐sheet interior will cross either an ice slab region, or an aquifer, or both.
Dust Source Activation Frequency in the Horn of Africa
Authors: Kunkelova Tereza , Crocker Anya J. , Wilson Paul A. , Schepanski Kerstin
Published: February 2024

Mineral dust aerosols play an important role in Earth's climate through interactions with incoming solar radiation, clouds, and the atmosphere. However, dust sources in the Horn of Africa (HoA) and controls on their activation are poorly documented. Here, we use fifteen‐minute Meteosat Second Generation Spinning Enhanced Visible and Infrared Imager dust index images to identify HoA dust source areas and to quantify their activation frequency in 1° × 1° resolution from 2006 to 2010. Around half of all recorded dust events occur in boreal summer, mostly between 8:00 and 16:00 local time. They are driven by meso‐ to regional scale meteorological mechanisms including the breakdown of the nocturnal low‐level jets, land‐sea breezes, and haboobs. By far the most dust‐active region in the HoA is the Afar Triangle (>77% of all recorded dust events) which features the Afar and Danakil depressions and is fed by the Awash River. Despite experiencing strong and persistent southwest summer monsoon winds, dust activation on the Somali Peninsula is less significant. A composite of our map with data for North Africa and westernmost Asia shows that the HoA is a striking latitudinal anomaly with dust activation extending deep into the equatorial belt. Our data also reveal that dust activation is unusually seasonal with ∼40% of events occurring in June and July. Our findings show that aridity and mean wind strength alone are poor predictors of dust activation and underscore the strong control exerted by the availability of readily deflated unconsolidated riverine and lacustrine sediments.
Increasing Occurrence of Sudden Turns From Drought to Flood Over China
Authors: Wang Hao , Wang Shanshan , Shu Xinya , He Yongli , Huang Jianping
Published: February 2024

This study focuses on a new compounding concern, the sudden turn from drought to flood (STDF), that is becoming increasingly prominent. This study investigates the long‐term trends and variability of STDFs in China during 1961–2020. The findings indicate that STDFs are prevalent in north and northeast China, and the Yangtze River Delta (YRD). The probability of a drought being followed by a severe flood is approaching 35% in northern and northeastern China. Since 1961, the number of STDFs in China has increased at a rate of average 2.8 events per decade. This increase mainly has occurred in late spring and early summer. The likelihood that a drought being followed by a significant pluvial is also increasing. The increasing STDF in northern China is mainly attributable to the increasing flood frequency and volatility of precipitation. Changes in the STDF trend is more dependent on the drought frequency in the YRD.
Mapping Geoelectric Field Hazards in Ireland
Authors: Malone‐Leigh John , Campanyà Joan , Gallagher Peter T. , Hodgson Jim , Hogg Colin
Published: February 2024

Geoelectric fields are generated at the Earth's surface and can lead to the induction of hazardous geomagnetically induced currents (GIC) in infrastructure like power grids, railways and pipelines during geomagnetic storms. Magnitude and orientation of the geoelectric fields, in relation to the infrastructure, are key features needed to determine the intensity of GIC. Here, we developed the first geoelectric hazard map for the island of Ireland, with the aim of providing detailed information that can help stakeholders mitigate the impact of GICs. The hazard map was developed by modeling and mapping the geoelectric field across Ireland for 28 years (1991–2018) using magnetic field data with magnetotelluric transfer functions. The approach for developing the hazard map calculates the probability of exceeding a hazardous geoelectric field threshold (500 mV/km) during large geomagnetic storms, taking directionality and amplitude into account. We found hazardous geoelectric fields to be mostly localized in areas in the west, south‐west and northern coast. We observed that the geoelectric field have a stronger dominant orientation than the orientation of the geomagnetic field, often constraining the hazardous geoelectric field in particular directions only. We demonstrate a seasonal/diurnal effect is present in the geoelectric field time series. The impact of galvanic distortion was also assessed, and we demonstrate that there is a significant difference in terms of amplitude and direction between both models.
Targeting the m6A RNA methyltransferase METTL3 attenuates the development of kidney fibrosis
Authors: Jung Hae Rim , Lee Jeonghwan , Hong Seung-Pyo , Shin Nayeon , Cho Ara , Shin Dong-Jin , Choi Jin Woo , Kim Jong-Il , Lee Jung Pyo , Cho Sung-Yup
Published: February 2024

Kidney fibrosis is a major mechanism underlying chronic kidney disease (CKD). N6-methyladenosine (m6A) RNA methylation is associated with organ fibrosis. We investigated m6A profile alterations and the inhibitory effect of RNA methylation in kidney fibrosis in vitro (TGF-β-treated HK-2 cells) and in vivo (unilateral ureteral obstruction [UUO] mouse model). METTL3-mediated signaling was inhibited using siRNA in vitro or the METTL3-specific inhibitor STM2457 in vivo and in vitro. In HK-2 cells, METTL3 protein levels increased in a dose- and time-dependent manner along with an increase in the cellular m6A levels. In the UUO model, METTL3 expression and m6A levels were significantly increased. Transcriptomic and m6A profiling demonstrated that epithelial-to-mesenchymal transition- and inflammation-related pathways were significantly associated with RNA m6A methylation. Genetic and pharmacologic inhibition of METTL3 in HK-2 cells decreased TGF-β-induced fibrotic marker expression. STM2457-induced inhibition of METTL3 attenuated the degree of kidney fibrosis in vivo. Furthermore, METTL3 protein expression was significantly increased in the tissues of CKD patients with diabetic or IgA nephropathy. Therefore, targeting alterations in RNA methylation could be a potential therapeutic strategy for treating kidney fibrosis.
Cathepsin D promotes polarization of tumor-associated macrophages and metastasis through TGFBI-CCL20 signaling
Authors: Lee Seul Gi , Woo Seon Min , Seo Seung Un , Lee Chan-Hyeong , Baek Moon-Chang , Jang Se Hwan , Park Zee Yong , Yook Simmyung , Nam Ju-Ock , Kwon Taeg Kyu
Published: February 2024

M2-like tumor-associated macrophages (TAMs) are risk factors for cancer progression and metastasis. However, the mechanisms underlying their polarization are still not fully understood. Although cathepsin D (Cat D) has been reported as a procarcinogenic factor, little is known about the functional role of Cat D in the tumor microenvironment (TME). This study aimed to explore the effect and molecular mechanisms of Cat D in the TME. Cat D knockout (KO) altered the cytokine secretion pattern and induced TAM reprogramming from the M2 to M1 subtype, thereby preventing epithelial-mesenchymal transition and tumor metastasis. Mechanistically, we identified transforming growth factor beta-induced protein (TGFBI) as a Cat D target protein that is specifically associated with TAM polarization. Elevated TGFBI expression in Cat D KO cancer cells resulted in a decline in M2-like TAM polarization. Our RNA-sequencing results indicated that the cancer cell-secreted chemokine CCL20 is a major secretory chemokine for Cat D-TGFBI-mediated TAM polarization. In contrast, Cat D overexpression accelerated TAM polarization into M2-like cells by suppressing TGFBI expression. In addition, the double Cat D and TGFBI KO rescued the inhibitory effects of Cat D KO on tumor metastasis by controlling TAM and T-cell activation. These findings indicated that Cat D contributes to cancer metastasis through TGFBI-mediated TAM reprogramming. Cat D deletion inhibits M2-like TAM polarization through TGFBI-mediated CCL20 expression, reprogramming the immunosuppressive TME. Our results open a potential new avenue for therapy focused on eliminating tumor metastasis.
Exploring the potential of Toxoplasma gondii in drug development and as a delivery system
Authors: Yoon Chanjin , Ham Yu Seong , Gil Woo Jin , Yang Chul-Su
Published: February 2024

Immune-mediated inflammatory diseases are various groups of conditions that result in immune system disorders and increased cancer risk. Despite the identification of causative cytokines and pathways, current clinical treatment for immune-mediated inflammatory diseases is limited. In addition, immune-mediated inflammatory disease treatment can increase the risk of cancer. Several previous studies have demonstrated that Toxoplasma gondii manipulates the immune response by inhibiting or stimulating cytokines, suggesting the potential for controlling and maintaining a balanced immune system. Additionally, T. gondii also has the unique characteristic of being a so-called “Trojan horse” bacterium that can be used as a drug delivery system to treat regions that have been resistant to previous drug delivery therapies. In this study, we reviewed the potential of T. gondii in drug development and as a delivery system through current research on inflammation-regulating mechanisms in immune-mediated inflammatory diseases.
Population genomics of the white-beaked dolphin (Lagenorhynchus albirostris): Implications for conservation amid climate-driven range shifts
Authors: Gose Marc-Alexander , Humble Emily , Brownlow Andrew , Wall Dave , Rogan Emer , Sigurðsson Guðjón Már , Kiszka Jeremy J. , Thøstesen Charlotte Bie , IJsseldijk Lonneke L. , ten Doeschate Mariel , Davison Nicholas J. , Øien Nils , Deaville Rob , Siebert Ursula , Ogden Rob
Published: February 2024

Climate change is rapidly affecting species distributions across the globe, particularly in the North Atlantic. For highly mobile and elusive cetaceans, the genetic data needed to understand population dynamics are often scarce. Cold-water obligate species such as the white-beaked dolphin (Lagenorhynchus albirostris) face pressures from habitat shifts due to rising sea surface temperatures in addition to other direct anthropogenic threats. Unravelling the genetic connectivity between white-beaked dolphins across their range is needed to understand the extent to which climate change and anthropogenic pressures may impact species-wide genetic diversity and identify ways to protect remaining habitat. We address this by performing a population genomic assessment of white-beaked dolphins using samples from much of their contemporary range. We show that the species displays significant population structure across the North Atlantic at multiple scales. Analysis of contemporary migration rates suggests a remarkably high connectivity between populations in the western North Atlantic, Iceland and the Barents Sea, while two regional populations in the North Sea and adjacent UK and Irish waters are highly differentiated from all other clades. Our results have important implications for the conservation of white-beaked dolphins by providing guidance for the delineation of more appropriate management units and highlighting the risk that local extirpation may have on species-wide genetic diversity. In a broader context, this study highlights the importance of understanding genetic structure of all species threatened with climate change-driven range shifts to assess the risk of loss of species-wide genetic diversity.
Associations of the utilization of household water treatment devices with mortality
Authors: Huang Shuai-Wen , Zhou Hong-Lian , Zeng Rui , Yao Ying
Published: February 2024

This study aimed to investigate the relationship between the utilization of household water treatment devices (UHWTD) and both overall and cause-specific mortality, addressing the lack of comprehensive prospective research in this area. The study was a population-based prospective cohort study using data from the US National Health and Nutrition Examination Survey (NHANES 1999–2010). Among the 30,322 participants included in the study, the group without UHWTD (No UHWTD) was younger but reported worse baseline health compared to the UHWTD group. During an average follow-up period of 14.3 years, 6811 participants died. After adjusting for age, sex, and other covariates, No UHWTD was significantly associated with a higher risk of overall (adjusted hazard ratio [aHR]: 1.221, 95% Confidence Interval [CI]: 1.147–1.300), heart (aHR: 1.405, 95% CI: 1.241–1.589), cancer (aHR: 1.176, 95% CI: 1.003–1.379), Alzheimer’s disease (aHR: 1.404, 95% CI: 1.027–1.919), and nephrosis (aHR: 1.613, 95% CI: 1.026–2.537) mortality compared to UHWTD. Moreover, those main association almost remained after further adjustment for water source. Additionally, we observed a lower detection rate of bromoform, chloroform, bromodichloromethane, and dibromochloromethane in tap water when household water treatment devices was used compared to No UHWTD. The utilization of household water treatment devices in this prospective cohort was associated with modest reductions in the risk of overall, heart, cancer, Alzheimer’s disease, and nephrosis mortality. These findings suggest that the use of household water treatment devices may have the potential to influence health outcomes and extend life expectancy.
Quenched lattice fluctuations in optically driven SrTiO3
Authors: Fechner M. , Först M. , Orenstein G. , Krapivin V. , Disa A. S. , Buzzi M. , von Hoegen A. , de la Pena G. , Nguyen Q. L. , Mankowsky R. , Sander M. , Lemke H. , Deng Y. , Trigo M. , Cavalleri A.
Published: February 2024

Crystal lattice fluctuations, which are known to influence phase transitions of quantum materials in equilibrium, are also expected to determine the dynamics of light-induced phase changes. However, they have only rarely been explored in these dynamical settings. Here we study the time evolution of lattice fluctuations in the quantum paraelectric SrTiO3, in which mid-infrared drives have been shown to induce a metastable ferroelectric state. Crucial in these physics is the competition between polar instabilities and antiferrodistortive rotations, which in equilibrium frustrate the formation of long-range ferroelectricity. We make use of high-intensity mid-infrared optical pulses to resonantly drive the Ti–O-stretching mode at 17 THz, and we measure the resulting change in lattice fluctuations using time-resolved X-ray diffuse scattering at a free-electron laser. After a prompt increase, we observe a long-lived quench in R-point antiferrodistortive lattice fluctuations. Their enhancement and reduction are theoretically explained by considering the fourth-order nonlinear phononic interactions to the driven optical phonon and third-order coupling to lattice strain, respectively. These observations provide a number of testable hypotheses for the physics of light-induced ferroelectricity.
Longevity of companion dog breeds: those at risk from early death
Authors: McMillan Kirsten M. , Bielby Jon , Williams Carys L. , Upjohn Melissa M. , Casey Rachel A. , Christley Robert M.
Published: February 2024

The companion dog is one of the most phenotypically diverse species. Variability between breeds extends not only to morphology and aspects of behaviour, but also to longevity. Despite this fact, little research has been devoted to assessing variation in life expectancy between breeds or evaluating the potential for phylogenetic characterisation of longevity. Using a dataset of 584,734 unique dogs located within the UK, including 284,734 deceased, we present variation in longevity estimates within the following: parental lineage (purebred = 1 breed, crossbred ≥ 2 breeds), breed (n = 155), body size (large, medium, small), sex (male, female) and cephalic index (brachycephalic, mesocephalic, dolichocephalic). Survival estimates were then partitioned amongst phylogenetic clades: providing evidence that canine evolutionary history (via domestication and associated artificial selection) is associated with breed lifespan. This information provides evidence to inform discussions regarding pedigree health, whilst helping current/prospective owners, breeders, policy makers, funding bodies and welfare organisations improve decision making regarding canine welfare.
microRNA blood signature for localized radiation injury
Authors: Ancel Lucie , Gabillot Olivier , Szurewsky Chloé , Granger Romain , Sache Amandine , Voyer Frédéric , Gruel Gaëtan , Illiano Stéphane , Benderitter Marc , Le Guen Bernard , Souidi Maâmar , Benadjaoud Mohamed Amine , Flamant Stéphane
Published: February 2024

A radiological accident, whether from industrial, medical, or malicious origin, may result in localized exposure to high doses of ionizing radiations, leading to the development of local radiation injury (LRI), that may evolve toward deep ulceration and necrosis of the skin and underlying tissues. Early diagnosis is therefore crucial to facilitate identification and management of LRI victims. Circulating microRNAs (miRNA) have been studied as potential diagnostic biomarkers of several diseases including hematological defects following whole-body irradiation (WBI). This study aims to identify a blood miRNA signature associated with LRI in a preclinical C57BL/6J mouse model of hindlimb irradiation using different 10-MV X-ray doses that lead to injuries of different severities. To this end, we first performed broad-spectrum plasma miRNA profiling, followed by a targeted validation step, on two independent animal cohorts. Using a multivariate sparse partial least square discriminant analysis, we identified a panel of eight circulating miRNAs able to segregate mice according to LRI severity. Interestingly, these miRNAs were previously associated with WBI (miR-150-5p, miR-342-3p, miR-146a-5p), inflammation (miR-18a-5p, miR-148b-3p, miR-532-5p) and skin diseases (miR-139-5p, miR-195-5p). Our results suggest the use of circulating miRNAs as suitable molecular biomarkers for LRI prognosis and diagnosis.
Spatiotemporal patterns of throwing muscle synergies in yips-affected baseball players
Authors: Aoyama Toshiyuki , Ae Kazumichi , Taguchi Takahiro , Kawamori Yuna , Sasaki Daisuke , Kawamura Takashi , Kohno Yutaka
Published: February 2024

“Yips” are involuntary movements that interfere with the automatic execution of sports movements. However, how the coordination among the various muscles necessary for sports movements is impaired in athletes with yips remains to be fully understood. This study aimed to assess whether muscle synergy analysis through non-negative matrix factorization (NMF) could identify impaired spatiotemporal muscle coordination in baseball players with throwing yips. Twenty-two college baseball players, including 12 with and 10 without yips symptoms participated in the study. Electromyographic activity was recorded from 13 ipsilateral upper extremity muscles during full-effort throwing. Muscle synergies were extracted through NMF. Cluster analysis was conducted to identify any common spatiotemporal patterns of muscle synergies in players with yips. Whether individual players with yips showed deviations in spatiotemporal patterns of muscle synergies compared with control players was also investigated. Four muscle synergies were extracted for each player, but none were specific to the yips group. However, a more detailed analysis of individual players revealed that two of the three players who presented dystonic symptoms during the experiment exhibited specific patterns that differed from those in control players. By contrast, each player whose symptoms were not reproduced during the experiment presented spatiotemporal patterns of muscle synergies similar to those of the control group. The results of this study indicate no common spatiotemporal pattern of muscle synergies specific to the yips group. Furthermore, these results suggest that the spatiotemporal pattern of muscle synergies in baseball throwing motion is not impaired in situations where symptoms are not reproduced even if the players have yips symptoms. However, muscle synergy analysis can identify the characteristics of muscle coordination of players who exhibit dystonic movements. These findings can be useful in developing personalized therapeutic strategies based on individual characteristics of yips symptoms.
Leptin haploinsufficiency exerts sex-dependent partial protection in SOD1G93A mice by reducing inflammatory pathways in the adipose tissue
Authors: Fernández-Beltrán Luis C. , Ali Zeinab , Larrad-Sanz Angélica , Lopez-Carbonero Juan I. , Godoy-Corchuelo Juan M. , Jimenez-Coca Irene , Garcia-Toledo Irene , Bentley Liz , Gomez-Pinedo Ulises , Matias-Guiu Jordi A. , Gil-Moreno Maria Jose , Matias-Guiu Jorge , Corrochano Silvia
Published: February 2024

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by significant metabolic disruptions, including weight loss and hypermetabolism in both patients and animal models. Leptin, an adipose-derived hormone, displays altered levels in ALS. Genetically reducing leptin levels (Lepob/+) to maintain body weight improved motor performance and extended survival in female SOD1G93A mice, although the exact molecular mechanisms behind these effects remain elusive. Here, we corroborated the sexual dimorphism in circulating leptin levels in ALS patients and in SOD1G93A mice. We reproduced a previous strategy to generate a genetically deficient leptin SOD1G93A mice (SOD1G93ALepob/+) and studied the transcriptomic profile in the subcutaneous adipose tissue and the spinal cord. We found that leptin deficiency reduced the inflammation pathways activated by the SOD1G93A mutation in the adipose tissue, but not in the spinal cord. These findings emphasize the importance of considering sex-specific approaches in metabolic therapies and highlight the role of leptin in the systemic modulation of ALS by regulating immune responses outside the central nervous system.
A comparative study on vildagliptin brand and its generic equivalents using dissolution test as quality control measure tool
Authors: Altoum Ghadah H. , AL-Enazi Fouza K. , Abudahash Mubarl M. , AL-Fadhli Reem A. , Alenzi Naif
Published: February 2024

Market drugs including brand or generic with poor quality, don’t meet the acceptable standard guidelines. Vildagliptin is an important antidiabetic drugs used in monotherapy or in combinations protocols for treatment of diabetes mellites. The main goal of the current study is to assess the pharmaceutical equivalence of two marketed generics of vildagliptin 50 mg tablets compared to the branded product (Galvus 50 mg). The in vitro dissolution test was used as a quality control tool to obtain the dissolution profile of vildagliptin compared to the reference drug. The results revealed that all tested samples showed dissolution behavior like standard drug. Whole samples dissolution reached after 15 min in accordance with the standard. According to the similarity factors records, tested vildagliptin samples showed a comparable dissolution to the reference drug. The current work presents an in vitro protocol for quality evaluation of recently released generic drugs.
Enhancing particle focusing: a comparative experimental study of modified square wave and square wave microchannels in lift and Dean vortex regimes
Authors: Ashkani Ali , Jafari Azadeh , Ghomsheh Mehryar Jannesari , Dumas Norbert , Funfschilling Denis
Published: February 2024

Serpentine microchannels are known for their effective particle focusing through Dean flow-induced rotational effects, which are used in compact designs for size-dependent focusing in medical diagnostics. This study explores square serpentine microchannels, a geometry that has recently gained prominence in inertial microfluidics, and presents a modification of square wave microchannels for improved particle separation and focusing. The proposed modification incorporates an additional U-shaped unit to convert the square wave microchannel into a non-axisymmetric structure, which enhances the Dean flow and consequently increases the Dean drag force. Extensive experiments were conducted covering a wide range of Reynolds numbers and particle sizes (2.45 µm to 12 µm). The particle concentration capability and streak position dynamics of the two structures were compared in detail. The results indicate that the modified square-wave microchannel exhibits efficient particle separation in the lower part of the Dean vortex-dominated regime. With increasing Reynolds number, the particles are successively focused into two streaks in the lift force-dominated regime and into a single streak in the Dean vortex-dominated regime, in this modified square wave geometry. These streaks have a low standard deviation around a mean value. In the Dean vortex-dominated regime, the location of the particle stream is highly dependent on the particle size, which allows good particle separation. Particle focusing occurs at lower Reynolds numbers in both the lift-dominated and lift/Dean drag-dominated regions than in the square wave microchannel. The innovative serpentine channel is particularly useful for the Dean drag-dominated regime and introduces a unique asymmetry that affects the particle focusing dynamics. The proposed device offers significant advantages in terms of efficiency, parallelization, footprint, and throughput over existing geometries.
Global burden and risk factors of gastritis and duodenitis: an observational trend study from 1990 to 2019
Authors: Liu Yupei , Zhang Jixiang , Guo Yingyun , Tian Shan , Wu Yanrui , Liu Chuan , Huang Xiaoyu , Zhang Shufei , Dong Weiguo
Published: February 2024

In recent years, there has been a global trend of aging, which has resulted in significant changes to the burden of gastritis and duodenitis (GD). Using the global burden of disease (GBD) database spanning 1990 to 2019, we evaluated the temporal trends of age-standardized incidence rates (ASIR), age-standardized death rates (ASDR), and age-standardized disability-adjusted life years (AS-DALYs) for GD using estimated annual percentage changes (EAPC). Additionally, we examined the burden of GD across various strata, including social demographic index (SDI), age, and sex. Finally, the risk factors linked to the incidence and mortality of GD, utilizing Pearson correlation analysis. In 2019, there were 31 million GD patients globally, a notable increase of 12 million from 1990, while the ASIR, ASDR, and AS-DALYs for GD all showed a decrease. Correlation analysis showed a significant negative relationship between ASIR and SDI. Factors like hand hygiene and vitamin A deficiency had significant positive correlations with ASIR and ASDR in 2019. Over the past thirty years, the burden of GD has increased alongside global population aging. Future efforts should focus on exploring prevention for GD, with special attention to the elderly population in low SDI regions.
Modulating the skin mycobiome-bacteriome and treating seborrheic dermatitis with a probiotic-enriched oily suspension
Authors: Truglio Mauro , Sivori Francesca , Cavallo Ilaria , Abril Elva , Licursi Valerio , Fabrizio Giorgia , Cardinali Giorgia , Pignatti Marco , Toma Luigi , Valensise Floriana , Cristaudo Antonio , Pimpinelli Fulvia , Di Domenico Enea Gino
Published: February 2024

Seborrheic dermatitis (SD) affects 2–5% of the global population, with imbalances in the skin microbiome implicated in its development. This study assessed the impact of an oily suspension containing Lactobacillus crispatus P17631 and Lacticaseibacillus paracasei I1688 (termed EUTOPLAC) on SD symptoms and the skin mycobiome-bacteriome modulation. 25 SD patients were treated with EUTOPLAC for a week. Symptom severity and skin mycobiome-bacteriome changes were measured at the start of the treatment (T0), after seven days (T8), and three weeks post-treatment (T28). Results indicated symptom improvement post-EUTOPLAC, with notable reductions in the Malassezia genus. Concurrently, bacterial shifts were observed, including a decrease in Staphylococcus and an increase in Lactobacillus and Lacticaseibacillus. Network analysis highlighted post-EUTOPLAC instability in fungal and bacterial interactions, with increased negative correlations between Malassezia and Lactobacillus and Lacticaseibacillus genera. The study suggests EUTOPLAC's potential as a targeted SD treatment, reducing symptoms and modulating the mycobiome-bacteriome composition.
Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda with SMS reporting
Authors: Aruhomukama Dickson , Magiidu Walusimbi Talemwa , Katende George , Ebwongu Robert Innocent , Bulafu Douglas , Kasolo Rajab , Nakabuye Hellen , Musoke David , Asiimwe Benon
Published: February 2024

In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating the AMR crisis. To address this issue, this study aimed to adapt a one-step AMR testing protocol alongside an SMS (Short Message Service) result relay system (SRRS), with the potential to reduce the turnaround time for AMR testing and result communication from 4 days or more to 1 day in Ugandan clinical microbiology laboratories. Out of the 377 samples examined, 54 isolates were obtained. Notably, E. coli (61%) and K. pneumoniae (33%) were the most frequently identified, majority testing positive for ESBL. Evaluation of three AMR testing protocols revealed varying sensitivity and specificity, with Protocol A (ChromID ESBL-based) demonstrating high sensitivity (100%) but no calculable specificity, Protocol B (ceftazidime-based) showing high sensitivity (100%) and relatively low specificity (7.1%), and Protocol C (cefotaxime-based) exhibiting high sensitivity (97.8%) but no calculable specificity. ESBL positivity strongly correlated with resistance to specific antibiotics, including cefotaxime, ampicillin, and aztreonam (100%), cefuroxime (96%), ceftriaxone (93%), and trimethoprim sulfamethoxazole (87%). The potential of integrating an SRRS underscored the crucial role this could have in enabling efficient healthcare communication in AMR management. This study underscores the substantial potential of the tested protocols for accurately detecting ESBL production in clinical samples, potentially, providing a critical foundation for predicting and reporting AMR patterns. Although considerations related to specificity warrant careful assessment before widespread clinical adoption.