bluuebunny/crossref_metadata_2025 huggingface dataset:
Anthropogenic climate change versus internal climate variability: Impacts on Alpine snow cover
Authors: Willibald Fabian , Kotlarski Sven , Grêt-Regamey Adrienne , Ludwig Ralf
Published: April 2020

Abstract. Snow is a sensitive component of the climate system. In many parts of the world, water, stored as snow, is a vital resource for agriculture, tourism and the energy sector. As uncertainties in climate change assessments are still relatively large, it is important to investigate the interdependencies between internal climate variability and anthropogenic climate change and their impacts on snow cover. We use regional climate model data from a new single model large ensemble with 50 members (ClimEX LE) as driver for the physically based snow model SNOWPACK at eight locations across the Swiss Alps. We estimate the contribution of internal climate variability to uncertainties in future snow trends by applying a Mann-Kendall test for consecutive future periods of different lengths (between 30 and 100 years) until the end of the 21st century. Under RCP8.5, we find probabilities between 15 % and 50 % that there will be no significantly negative trend in future mean snow depths over a period of 50 years. While it is important to understand the contribution of internal climate variability to uncertainties in future snow trends, it is likely that the variability of snow depth itself changes with anthropogenic forcing. We find that relative to the mean, inter-annual variability of snow increases in the future. A decrease of future mean snow depths, superimposed by increases in inter-annual variability will exacerbate the already existing uncertainties that snow-dependent economies will have to face in the future.
The Evolution of the Duty to Consult
Authors: Gonzalez Alejandro
Published: April 2020

The duty to consult has been the subject of many trials and headlines, yet many still feel as though the consultation process is faulty. Relying on recent caselaw from the Supreme Court of Canada and the Federal Court of Appeal, I trace the evolution of the duty to consult from its inception to the latest major cases. I suggest that the current process can be improved to better engage all parties, (i.e. Crown, regulatory agency, industry proponent, Indigenous peoples), with a stronger emphasis on efficiency and reconciliation. I rely on the work of Matthew Hodgson to further solidify my consultation framework by advancing the idea of a specialized tribunal charged with reviewing the adequacy of consultations.
That Most Canadian of Virtues
Authors: Gray Kevin
Published: April 2020

In this paper, I argue that the use of comity by the Supreme Court in its Charter jurisprduence, particularly its Section 7 jurisprduence, has lacked any conceptual clarity or fidelity to international law. This paper aims to advance the scholarly literature in three ways: first, by developing a taxonomy of s.7 extraterritorial cases and second: by showing how comity has been developed in four key areas of the law I identify it as playing a role in: extradition cases, the use of foreign-obtained evidence, civil cases in Canada where Canadians may be exposed to foreign criminal prosecution, and in the so-called 'war on terror'. I conclude the paper by arguing, third, that the SCC has employed comity as an interpretive principle to produce a conceptual muddle wholly incompatible with the traditional rules of international law.
Immigration Detention and Habeas Corpus
Authors: Chowdhury Mannu
Published: April 2020

In Canada (Public Safety and Emergency Preparedness) v Chhina (“Chhina”), a majority of the Supreme Court of Canada found that immigration detainees in detention of lengthy and uncertain duration may access habeas corpus relief at provincial superior courts. The majority held that the detention review scheme under the governing statute did not accord as broad and advantageous protection as that provided by habeas corpus. The author argues that Chhina is a positive development with missed opportunities. The Supreme Court laudably clarified the case law and narrowed the exception that previously barred immigration detainees from seeking any form of habeas corpus relief. Further, in finding that the governing detention review scheme is not as broad and advantageous as habeas corpus, the majority adopted a pragmatic approach and focused on how detention reviews are conducted, not on how they ought to be performed. Notwithstanding these strengths, the majority missed an opportunity to resolve the conflicting case law on three common issues that arise when detainees in lengthy immigration detention file habeas corpus petitions: (i) determining when a detention becomes unduly lengthy such that it is unlawful; (ii) developing a singular approach to calculating the duration of detention; and (iii) establishing the proper weight that should be ascribed to detainee cooperation in habeas corpus applications. Moreover, in omitting substantive discussions of the broader context surrounding immigration detention, the majority missed a valuable opportunity to fully shine the light on the shortcomings of immigration detention and in the process potentially usher in systemic changes.
Sex & Sexuality Module I: Research Collections from the Kinsey Institute Library & Special Collections
Authors: Behrend Dawn
Published: April 2020

Sex & Sexuality, Module I: Research Collections from the Kinsey Institute Library & Special Collections published by Adam Matthew Digital is a collection of digitized primary sources obtained exclusively from the Kinsey Institute Library & Special Collections dedicated to
the study of human sexuality throughout the twentieth century. The collection makes use of the artificial intelligence capabilities of Handwritten Text Recognition (HTR) to enable keyword searching of handwritten documents. The documents and images in the collection have been meticulously
digitized by Adam Matthew Digital making them discoverable, visually appealing, and adjustable. The proprietary interface is intuitive to navigate with the product being compatible with a range of browsers and electronic devices. Contract provisions are standard to the product and permit for
use across locations and interlibrary loan sharing. As pricing is primarily determined by size and enrollment, the collection may be affordable for libraries of varying sizes. Users seeking more current research on gender and women’s studies may find ProQuest’s GenderWatch a more
suitable choice, while those seeking information on sexuality from the sixteenth to mid-twentieth centuries may prefer Part III of Gale’s Archives of Sexuality & Gender with both resources providing access to a range of sources beyond that of the Kinsey Institute.
Construction of Huawei’s Image in News Reports: Take China Daily and The New York Times for Example
Authors: Ma Kuizi , Xiao Ya
Published: April 2020

In recent years, as China’s largest smartphone company, Huawei’s position in the international market has gradually increased and received widespread attention from foreign media. The rapid development of China’s impact on the hegemony of the US has also changed the direction of US media’s reporting on Chinese companies. At this stage, it is meaningful to study the image of Huawei in both Chinese and US media reports. Therefore, based on the corpus approach and critical discourse analysis, this paper builds two corpora of China Daily (576 reports with 438,261 words) and The New York Times (429 reports with 347,025 words). It is found that (1) both sides acknowledge that Huawei ranks top in world telecommunication technology, particularly in the 5G network; (2) two newspapers focus on different aspects in their reports. For the Chinese media, Huawei’s technological prowess, innovation capacity in the global market, cooperation with many other European and African countries are given more attention, while for the American media, more focus is shifted to Huawei’s threat to national security; (3) two newspapers hold different attitudes towards the rise of Huawei. China Daily’s positive construction of Huawei’s image is obvious. While for the American media, the Trump administration is more likely to project a threatening image of Huawei; (4) the reporting frameworks and the styles of materials selected differ in two newspapers. China Daily’s framework concentrates on “Huawei” itself, while The New York Times tends to construct a reporting framework from multiple perspectives from the third-party.
/dˤ/-Variation in Saudi Newscasting and Phonological Theory
Authors: Al-Tamimi Yasser A.
Published: April 2020

In his analysis of /dˤ/-variation in Saudi Arabian newscasting, Al-Tamimi (2020) finds unpredicatble variability between the standard variant [dˤ] and the non-standard variant [ðˤ] in different in-words positions, in different phonetic environments, and in semantically ‘content’ and suprasegmentally ‘stressed’ lexical itmes assumed to favor the standard variant. He even finds in many of these lexical items an unusual realizational flucatuation between the two variants. The present exploratory and ‘theory-testing’ study aims to find a reasonable account for these findings through examining the explanatory adequacy of a number of available phonological theories, notions, models and proposals that have made different attempts to accommodate variation, and this includes Coexistent Phonemic Systems, Standard Generative Phonology, Lexical Diffusion, Variable Rules, Poly-Lectal Grammar, Articulatory Phonology, different versions of the Optimality Theory, in addition to the Multiple-Trace-Model, as represented by Al-Tamimi’s (2005) Multiple-Trace-Based Proposal. The study reveals the strengths and weaknesses of these theories in embracing the variability in the data, and concludes that the Multiple-Trace-Based Proposal can relatively offer the best insight as its allows variation to be directly encoded in the underlying representations of lexical items, a status strictly prohibited by the rest of the theories that adopt invariant lexical representations in consonance with the ‘Homogeneity Doctrine’.
Influence of Phosphorus Fertilizer on Potato Seed Production in Acid Soils in Kenya
Authors: Akoto Evans Mutange , Othieno Caleb O. , Ochuodho Julius O.
Published: April 2020

One of the major challenges facing potato (Solanum Tuberosum L) production in Kenya is the inadequate supply of high-quality seed. The required amount of potato seed in Kenya is about 70,000 tones annually but, only 1 % is available. Otherwise, farmers use low quality farm saved seed (KEPHIS, 2016). A major contributor to this situation is low and declining soil fertility, particularly phosphorus, among other challenges. Unfortunately, there is no available phosphorus fertilizer rate recommendation for seed potato production in Kenya. This hinders economic utilization of phosphorus fertilizers to achieve optimal production of quality potato seed in Kenya where its deficiencies are dominant. Therefore, this study investigated influence of different rates of phosphorus fertilizer on seed potato tuber yield and quality in three acidic (pH ≤ 5.8) test sites: Lari, Ainabkoi and Saboti sub Counties. Unica and Shangi varieties were tested. The field experiment was a split plot arrangement in Randomised Complete Block Design (RCBD) with six treatments (0 N & 0 P), 0, 30, 60, 90 and 120 kg ha-1 phosphorus, replicated three times. Data collected included tuber weight, number of tubers, tuber grade, number of eyes per tuber, tuber specific density and final germination percentage. To monitor soil nutrient dynamics, soil pH, soil available phosphorus, total nitrogen, total carbon, potassium, calcium, magnesium, copper, iron, zinc, sodium and exchangeable acidity (Lari only) were determined at planting while available phosphorus and potato tuber phosphorus were determined at harvesting. Data was statistically analysed using ANOVA at 5 % confidence levels with General Statistics (GENSTAT) and excel softwares. Results indicated that phosphorus rate significantly influenced seed tuber yield in the test sites. At Saboti, (0N & 0P) and no phosphorus application treatments for Shangi and Unica resulted in the highest seed tuber yield of 33.7 t ha-1 and 33.2 t ha-1, respectively. At Ainabkoi, application of 60 kg ha-1 and 30 kg ha-1 phosphorus produced the highest seed tuber yields of 20.0 t ha-1 and 18.9 t ha-1 of Shangi and Unica, respectively. In Lari, application of 60 kg ha-1 and 90 kg ha-1 phosphorus produced the highest seed tuber yields of 19.0 t ha-1 and 10.4 t ha-1 of Shangi and Unica, respectively. Unica had better final germination percentage than Shangi. During the season, there was a build-up of soil available phosphorus. Thus, there is need for farmers to test their soils at the onset of every potato season.
Evaluation of liquid and powdered forms of polyclonal antibody preparation against Streptococcus bovis and Fusobacterium necrophorum in cattle adapted or not adapted to highly fermentable carbohydrate diets
Authors: Cassiano Eduardo Cuellar Orlandi , Junior Flavio Perna , Barros Tarley Araújo , Marino Carolina Tobias , Pacheco Rodrigo Dias Lauritano , Ferreira Fernanda Altieri , Millen Danilo Domingues , Martins Mauricio Furlan , Pugine Silvana Marina Piccoli , de Melo Mariza Pires , Beauchemin Karen Ann , Meyer Paula Marques , Arrigoni Mario de Beni , Rodrigues Paulo Henrique Mazza
Published: April 2020

Objective: Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet.Methods: A double 3×3 Latin square design was used with three PAP treatments (control, powdered, and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly.Results: Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of Streptococcus bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals.Conclusion: Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.
Genetic evaluation of sheep for resistance to gastrointestinal nematodes and body size including genomic information
Authors: Torres Tatiana Saraiva , Sena Luciano Silva , Santos Gleyson Vieira dos , Figueiredo Filho Luiz Antonio Silva , Barbosa Bruna Lima , Júnior Antônio de Sousa , Britto Fábio Barros , Sarmento José Lindenberg Rocha
Published: April 2020

Objective: The genetic evaluation of Santa Inês sheep was performed for resistance to gastrointestinal nematode infection (RGNI) and body size using different relationship matrices to assess the efficiency of including genomic information in the analyses.Methods: There were 1,637 animals in the pedigree and 500, 980, and 980 records of RGNI, thoracic depth (TD), and rump height (RH), respectively. The genomic data consisted of 42,748 SNPs and 388 samples genotyped with the OvineSNP50 BeadChip. The (co)variance components were estimated in single- and multi-trait analyses using the numerator relationship matrix (A) and the hybrid matrix H, which blends A with the genomic relationship matrix (G). The BLUP and single-step genomic BLUP methods were used. The accuracies of estimated breeding values and Spearman rank correlation were also used to assess the feasibility of incorporating genomic information in the analyses.Results: The heritability estimates ranged from 0.11±0.07, for TD (in single-trait analysis using the A matrix), to 0.38±0.08, for RH (using the H matrix in multi-trait analysis). The estimates of genetic correlation ranged from –0.65±0.31 to 0.59±0.19, using A, and from –0.42±0.30 to 0.57±0.16 using H. The gains in accuracy of estimated breeding values ranged from 2.22% to 75.00% with the inclusion of genomic information in the analyses.Conclusion: The inclusion of genomic information will benefit the direct selection for the traits in this study, especially RGNI and TD. More information is necessary to improve the understanding on the genetic relationship between resistance to nematode infection and body size in Santa Inês sheep. The genetic evaluation for the evaluated traits was more efficient when genomic information was included in the analyses.
Variance component analysis of growth and production traits in Vanaraja male line chickens using animal model
Authors: Ullengala Rajkumar , Prince L. Leslie Leo , Paswan Chandan , Haunshi Santosh , Chatterjee Rudranath
Published: April 2020

Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken.Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five generations data to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits in PD-1 chickens. Genetic trend was estimated by regression of the estimated average breeding values (BV) on generations.Results: The body weight (BW) and shank length (SL) varied significantly (p≤0.01) among the generations, hatches and sexes. The least squares mean of SL at six weeks, the primary trait was 77.44±0.05 mm. All the production traits, viz., BWs, age at sexual maturity, egg production (EP) and egg weight were significantly influenced by generation. Model four with additive, maternal permanent environmental and residual effects was the best model for juvenile growth traits, except for zero-day BW. The heritability estimates for BW and SL at six weeks (SL6) were 0.20±0.03 and 0.17±0.03, respectively. The BV of SL6 in the population increased linearly from 0.03 to 3.62 mm due to selection. Genetic trend was significant (p≤0.05) for SL6, BW6, and production traits. The average genetic gain of EP40 for each generation was significant (p≤0.05) with an average increase of 0.38 eggs per generation. The average inbreeding coefficient was 0.02 in PD-1 line.Conclusion: The population was in ideal condition with negligible inbreeding and the selection was quite effective with significant genetic gains in each generation for primary trait of selection. The animal model minimized the over-estimation of genetic parameters and improved the accuracy of the BV, thus enabling the breeder to select the suitable breeding strategy for genetic improvement.
Estimating genetic diversity and population structure of 22 chicken breeds in Asia using microsatellite markers
Authors: Roh Hee-Jong , Kim Seung-Chang , Cho Chang-Yeon , Lee Jinwook , Jeon Dayeon , Kim Dong-kyo , Kim Kwan-Woo , Afrin Fahmida , Ko Yeoung-Gyu , Lee Jun-Heon , Batsaikhan Solongo , Susanti Triana , Hegay Sergey , Kongvongxay Siton , Gorkhali Neena Amatya , Thi Lan Anh Nguyen , Thao Trinh Thi Thu , Manikku Lakmalie
Published: April 2020

Objective: Estimating the genetic diversity and structures, both within and among chicken breeds, is critical for the identification and conservation of valuable genetic resources. In chickens, microsatellite (MS) marker polymorphisms have previously been widely used to evaluate these distinctions. Our objective was to analyze the genetic diversity and relationships among 22 chicken breeds in Asia based on allelic frequencies.Methods: We used 469 genomic DNA samples from 22 chicken breeds from eight Asian countries (South Korea, KNG, KNB, KNR, KNW, KNY, KNO; Laos, LYO, LCH, LBB, LOU; Indonesia, INK, INS, ING; Vietnam, VTN, VNH; Mongolia, MGN; Kyrgyzstan, KGPS; Nepal, NPS; Sri Lanka, SBC) and three imported breeds (RIR, Rhode Island Red; WLG, White Leghorn; CON, Cornish). Their genetic diversity and phylogenetic relationships were analyzed using 20 MS markers.Results: In total, 193 alleles were observed across all 20 MS markers, and the number of alleles ranged from 3 (MCW0103) to 20 (LEI0192) with a mean of 9.7 overall. The NPS breed had the highest expected heterozygosity (Hexp, 0.718±0.027) and polymorphism information content (PIC, 0.663±0.030). Additionally, the observed heterozygosity (Hobs) was highest in LCH (0.690±0.039), whereas WLG showed the lowest Hexp (0.372±0.055), Hobs (0.384±0.019), and PIC (0.325±0.049). Nei’s DA genetic distance was the closest between VTN and VNH (0.086), and farthest between KNG and MGN (0.503). Principal coordinate analysis showed similar results to the phylogenetic analysis, and three axes explained 56.2% of the variance (axis 1, 19.17%; 2, 18.92%; 3, 18.11%). STRUCTURE analysis revealed that the 22 chicken breeds should be divided into 20 clusters, based on the highest ΔK value (46.92).Conclusion: This study provides a basis for future genetic variation studies and the development of conservation strategies for 22 chicken breeds in Asia.
Prediction of net energy values in expeller-pressed and solvent-extracted rapeseed meal for growing pigs
Authors: Li Zhongchao , Lyu Zhiqian , Liu Hu , Liu Dewen , Jaworski Neil , Li Yakui , Lai Changhua
Published: April 2020

Objective: The objective of this study was to determine net energy (NE) of expeller-press (EP-RSM) and solvent-extracted rapeseed meal (SE-RSM) and to establish equations for predicting the NE in rapeseed meal (RSM) fed to growing pigs.Methods: Thirty-six barrows (initial body weight [BW], 41.1±2.2 kg) were allotted into 6 diets comprising a corn-soybean meal basal diet and 5 diets containing 19.50% RSM added at the expense of corn and soybean meal. The experiment had 6 periods and 6 replicate pigs per diet. During each period, the pigs were individually housed in metabolism crates for 16 days which included 7 days for adaption to diets. On day 8, pigs were transferred to respiration chambers and fed their respective diet at 2,000 kJ metabolizable energy (ME)/kg BW0.6/d. Feces and urine were collected, and daily heat production was measured from day 9 to 13. On days 14 and 15, the pigs were fed at 890 kJ ME/kg BW0.6/d and fasted on day 16 for evaluation of fasting heat production (FHP).Results: The FHP of pigs averaged 790 kJ/kg BW0.6/d and was not affected by the diet composition. The NE values were 10.80 and 8.45 MJ/kg DM for EP-RSM and SE-RSM, respectively. The NE value was positively correlated with gross energy (GE), digestible energy (DE), ME, and ether extract (EE). The best fit equation for NE of RSM was NE (MJ/kg DM) = 1.14×DE (MJ/kg DM)+0.46×crude protein (% of DM)–25.24 (n = 8, R2 = 0.96, p<0.01). The equation NE (MJ/kg DM) = 0.22×EE (% of DM)–0.79×ash (% of DM)+14.36 (n = 8, R2 = 0.77, p = 0.018) may be utilized to quickly determine the NE in RSM when DE or ME values are unavailable.Conclusion: The NE values of EP-RSM and SE-RSM were 10.80 and 8.45 MJ/kg DM. The NE value of RSM can be well predicted based on energy content (GE, DE, and ME) and proximate analysis.
Welfare traits of Bos indicus cattle castrated immunologically and fed beta-adrenergic agonists
Authors: Martello Luciane Silva , Antonelo Daniel Silva , Consolo Nara Regina Brandao , Pacheco Verônica Madeira , Negrão João Alberto , Rosa Alessandra Fernandes , Leme Paulo Roberto , Sousa Rafael Vieira , da Luz Silva Saulo
Published: April 2020

Objective: This work was carried out to evaluate the effects of zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH) combined with immunocastration on the welfare traits of feedlot Nellore cattle.Methods: Ninety-six Nellore males (average body weight [BW] = 409±50 kg; average 20 mo of age) were divided into two groups according to BW; half of the animals in each group received two doses of an immunocastration (ImC) vaccine in a 30 day interval, and the other half did not receive the vaccine (NoC). Afterward, the animals were housed and fed a common diet for 70 days. Then, they were split into three groups and fed one of the following diets for 30 additional days: control (CO) diet, with no β-AA; ZH diet, containing 80 mg/d ZH; and RH diet, containing 300 mg/d RH. Welfare traits were assessed by monitoring body surface temperature using infrared thermography (IRT) and plasma cortisol and temperament measurements.Results: There was no interaction between sexual condition and diet for any trait. The ImC and NoC groups did not differ in rectal and ocular temperatures. The ImC animals had higher flight speeds (p = 0.022) and tended to have higher cortisol levels (p = 0.059) than the NoC animals. Animals fed ZH and RH did not differ in cortisol levels, respiratory rate, rectal temperature, temperature measured by IRT, or temperament behaviour.Conclusion: The ImC animals showed a less stable temperament during handling practices than NoC, whereas ZH and RH supplementation had no adverse effects on animal welfare.
Distribution of injected fat-soluble vitamins in plasma and tissues of nursery pigs
Authors: Jang Young Dal , Rotering Mikayla  J. , Isensee Paige K. , Rinholen Kirsten  A. , Boston-Denton Carli J. , Kelley Paige G. , Stuart Robert L.
Published: April 2020

Objective: The objective of this experiment was to investigate the effects of fat-soluble vitamin injection on plasma and tissue vitamin status in nursery pigs.Methods: A total of 16 pigs (initial body weight: 7.15±1.1 kg) were allotted to 2 treatments at d 7 post-weaning. Pigs were fed a corn-soybean meal-based basal diet with no supplemental vitamin A and i.m. injected with 300,000 IU of retinyl palmitate, 900 IU of d-α-tocopherol and 30,000 IU of vitamin D3 with control pigs having no vitamin injection. Blood (d 0, 3, 7, and 14 post-injection) and tissue samples (liver, brain, heart, lung, and muscle; d 7 and 14 post-injection) were collected from pigs. Retinyl palmitate, retinol, and α-tocopherol concentrations were analyzed in plasma and tissues, while plasma was assayed for 25-hydroxycholecalciferol (25-OHD3).Results: Plasma retinol and 25-OHD3 concentrations increased by the vitamin injection from d 3 to 14 post-injection (p<0.05) whereas plasma retinyl palmitate was detected only in the vitamin treatment at d 3 and 7 post-injection (115.51 and 4.97 μg/mL, respectively). Liver retinol, retinyl palmitate, and retinol+retinyl palmitate concentrations increased by retinyl palmitate injection at d 7 and 14 post-injection (p<0.05) whereas those were not detected in the other tissues. The d-α-tocopherol injection increased α-tocopherol concentrations in plasma at d 3 and 7 post-injection (p<0.05) and in liver, heart (p<0.10), and muscle (p<0.05) at d 7 post-injection.Conclusion: Fat-soluble vitamin injection increased plasma status of α-tocopherol, retinol, retinyl palmitate and 25-OHD3. As plasma levels decreased post-injection, vitamin A level in liver and vitamin E level in muscle, heart and liver increased. The α-tocopherol found in plasma after injection was distributed to various tissues but retinyl palmitate only to the liver.
Regulation of chicken vanin1 gene expression by peroxisome proliferators activated receptor α and miRNA-181a-5p
Authors: Wang Zhongliang , Yu Jianfeng , Hua Nan , Li Jie , Xu Lu , Yao Wen , Gu Zhiliang
Published: April 2020

Objective: Vanin1 (VNN1) is a pantetheinase that can catalyze the hydrolysis of pantetheine to produce pantothenic acid and cysteamine. Our previous studies showed that VNN1 is specifically expressed in chicken liver. In this study, we aimed to investigate the roles of peroxisome proliferators activated receptor α (PPARα) and miRNA-181a-5p in regulating VNN1 gene expression in chicken liver.Methods: 5′-RACE was performed to identify the transcription start site of chicken VNN1. JASPAR and TFSEARCH were used to analyze the potential transcription factor binding sites in the promoter region of chicken VNN1 and miRanda was used to search miRNA binding sites in 3′ untranslated region (3′UTR) of chicken VNN1. We used a knock-down strategy to manipulate PPARα (or miRNA-181a-5p) expression levels in vitro to further investigate its effect on VNN1 gene transcription. Luciferase reporter assays were used to explore the specific regions of VNN1 targeted by PPARα and miRNA-181a-5p.Results: Sequence analysis of the VNN1 promoter region revealed several transcription factor-binding sites, including hepatocyte nuclear factor 1α (HNF1α), PPARα, and CCAAT/enhancer binding protein α. GW7647 (a specific agonist of PPARα) increased the expression level of VNN1 mRNA in chicken primary hepatocytes, whereas knockdown of PPARα with siRNA increased VNN1 mRNA expression. Moreover, the predicted PPARα-binding site was confirmed to be necessary for PPARα regulation of VNN1 gene expression. In addition, the VNN1 3′UTR contains a sequence that is completely complementary to nucleotides 1 to 7 of miRNA-181a-5p. Overexpression of miR-181a-5p significantly decreased the expression level of VNN1 mRNA.Conclusion: This study demonstrates that PPARα is an important transcriptional activator of VNN1 gene expression and that miRNA-181a-5p acts as a negative regulator of VNN1 expression in chicken hepatocytes.
Achyranthes japonica extracts supplementation to growing pigs positively influences growth performance, nutrient digestibility, fecal microbial shedding, and fecal gas emission
Authors: Liu Xiao , Lee Sang In , Kim In Ho
Published: April 2020

Objective: An experiment was conducted to investigate the effects of Achyranthes japonica extracts (AJE) on the growth performance, nutrient digestibility, fecal microbial shedding, and fecal gas emission of growing pigs.Methods: A total of 180 ([Landrace×Yorkshire]×Duroc) growing pigs with initial body weight (BW) of 23.94±1.54 kg were used in this study to investigate the effects of AJE as a feed additive. Dietary treatments included: i) CON (basal diet), ii) TRT1 (basal diet+0.05% AJE), and iii) TRT2 (basal diet+0.10% AJE).Results: As a result of the dietary supplementation of 0% to 0.10% AJE, a linear increase of BW (p<0.05) on d 21 and 42, a linear increase of average daily gain (ADG) (p<0.05) during d 21 to 42, a trend in linear increase of ADG (p<0.10) during d 0 to 21 and d 0 to 42, a linear increase of gain to feed ratio (G:F) (p<0.05) during d 0 to 42, and a tendency in the linear increase of G:F during d 21 to 42 were observed in this study. Additionally, dietary supplementation of 0% to 0.10% AJE had a linear increase (p<0.05) on the apparent total tract digestibility of dry matter (DM) and energy, a linear increase (p<0.05) on lactic acid bacteria counts, a tendency in reducing (linear effect, p<0.10) coliform bacteria counts, and a linear decrease (p<0.05) in excreta H2S emission content in growing pigs.Conclusion: In conclusion, the results suggested that AJE had the potential to enhance growth performance, DM and energy digestibility, and fecal lactic acid bacteria counts, and decrease the fecal coliform bacteria counts and excreta H2S emission in growing pigs.
Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
Authors: Susilowati Suherni , Sardjito Trilas , Mustofa Imam , Widodo Oky Setio , Kurnijasanti Rochmah
Published: April 2020

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.
CT-Guided Percutaneous Core Needle Biopsy for Lung Lesions: A 14-Year Review of Needle Angle and Lesion Depth
Authors: Lee Jee Hyeon , Lee In Jae
Published: April 2020

Background: Increasing success rate and reducing complications are important for computed tomography (CT)-guided percutaneous core needle biopsy (PCNB). Objectives: To assess the influence of needle angle and lesion depth on procedural success and complications of CT-guided PCNB for intrapulmonary lesions, performed by a single radiologist. Patients and Methods: A total of 689 cases of PCNB performed under CT guidance were enrolled in this study. The collected data were retrospectively reviewed. The pathologic results and complications were evaluated for each case. Two factors-needle angle and lesion depth-were statistically analyzed to assess the relationship with procedural success and complications of PCNB by using univariate analysis. Post hoc analysis was performed with Bonferroni’s method. Results: The overall success rate was 93.1% (642/689). Procedural success showed no statistically significant association with both needle angle (P = 0.568) and lesion depth (P = 0.144). The overall complication rate was 17.9% (123/689) with 15.7% for minor complications and 2.2% for major complications. The needle angle had no association with complications (P = 0.101). Presence and severity of complications showed a direct relationship with lesion depth (P < 0.01). In fact, more severe complications occurred in deeper located lesions. Conclusion: Needle angle had no effect on both procedural success and complications. Also, there was no significant correlation between lesion depth and procedural success. However, lesion depth was closely correlated with the incidence and severity of complications after PCNB.
Liver Transplantation Outcome in Iranian Patients with Primary Hyperoxaluria; Risks and Perspectives
Authors: Dehghani Seyed Mohsen , Bagheri Lankarani Kamran , Shahramian Iraj , Bazi Ali , Shahabi Hakimeh , Kazemi Kurosh , Gholami Siavash , Malek-Hosseini Seyed Ali
Published: April 2020

Background: Primary hyperoxaluria (PH) is an autosomal genetic disorder characterized by abnormal glycosylate metabolism. Objectives: The aim of the present study was to assess post-transplant complications and survival of patients with PH who underwent either liver transplantation (LT) or simultaneous liver-kidney transplantation. Methods: 18 patients with established PH diagnosis who underwent LT or simultaneous liver-kidney transplantation in the Transplantation Center of Shiraz, Iran, were included. Demographic and clinical data were collected by reviewing clinical documents and interviews by the patients. The patients were prospectively followed up for the occurrence of the intended outcomes. The data was analyzed by SPSS 18 software. Results: 12 patients (66.7%) were male, and six patients (33.3%) were female with the age range of 3 to 32 years (mean age, 18.89 ± 7.42 years). The patients’ weight ranged from 13 to 73 kg (mean weight, 47.39 ± 17.18 kg). Polyuria was the most common clinical presentation (11/18), and end-stage renal disease and hemodialysis were noted in 13 and 12 patients pre-transplantation, respectively. Hepatic arterial thrombosis, biliary complications, infections, and graft rejection comprised the most frequent post-transplant complications. Of 18 patients, seven patients (38.9%) died due to various complications during one year after transplant. Based on the Kaplan-Meier analysis, the survival rate was 61.1% at the end of the study. The mean survival time was 46.25 ± 18.6 months. The patients succumbed to the disease died within 3 to 320 days (mean, 61.57 days) post-surgery. Conclusions: LT seems an effective intervention in prevention of kidney failure in patients with PH.
The Matter of Facts
Authors: Leng Gareth , Leng Rhodri Ivor
Published: April 2020

How biases, the desire for a good narrative, reliance on citation metrics, and other problems undermine confidence in modern science.

Modern science is built on experimental evidence, yet scientists are often very selective in deciding what evidence to use and tend to disagree about how to interpret it. In The Matter of Facts, Gareth and Rhodri Leng explore how scientists produce and use evidence. They do so to contextualize an array of problems confronting modern science that have raised concerns about its reliability: the widespread use of inappropriate statistical tests, a shortage of replication studies, and a bias in both publishing and citing “positive” results. Before these problems can be addressed meaningfully, the authors argue, we must understand what makes science work and what leads it astray.

The myth of science is that scientists constantly challenge their own thinking. But in reality, all scientists are in the business of persuading other scientists of the importance of their own ideas, and they do so by combining reason with rhetoric. Often, they look for evidence that will support their ideas, not for evidence that might contradict them; often, they present evidence in a way that makes it appear to be supportive; and often, they ignore inconvenient evidence.

In a series of essays focusing on controversies, disputes, and discoveries, the authors vividly portray science as a human activity, driven by passion as well as by reason. By analyzing the fluidity of scientific concepts and the dynamic and unpredictable development of scientific fields, the authors paint a picture of modern science and the pressures it faces.
Keep Calm and Log On
Authors: Andrews Gillian "Gus"
Published: April 2020

How to survive the digital revolution without getting trampled: your guide to online mindfulness, digital self-empowerment, cybersecurity, creepy ads, trustworthy information, and more.

Feeling overwhelmed by an avalanche of online content? Anxious about identity theft? Unsettled by the proliferation of fake news? Welcome to the digital revolution. Wait—wasn't the digital revolution supposed to make our lives better? It was going to be fun and put the world at our fingertips. What happened? Keep Calm and Log On is a survival handbook that will help you achieve online mindfulness and overcome online helplessness—the feeling that tech is out of your control—with tips for handling cybersecurity, creepy ads, untrustworthy information, and much more.

Taking a cue from the famous World War II morale-boosting slogan (“Keep Calm and Carry On”), Gus Andrews shows us how to adapt the techniques our ancestors used to survive hard times, so we can live our best lives online. She explains why media and technology stress us out, and offers empowering tools for coping. Mindfulness practices can help us stay calm and conserve our attention purposefully. Andrews shares the secret of understanding our own opinions'' “family trees” in order to identify misleading “fake news.” She provides tools for unplugging occasionally, overcoming feelings that we are “bad at technology,” and taking charge of our security and privacy. Andrews explains how social media algorithms keep us from information we need and why “creepy ads” seem to follow us online. Most importantly, she urges us to work to rebuild the trust in our communities that the internet has broken.
Identification of hub genes and small-molecule compounds in medulloblastoma by integrated bioinformatic analyses
Authors: Liu Zhendong , Zhang Ruotian , Sun Zhenying , Yao Jiawei , Yao Penglei , Chen Xin , Wang Xinzhuang , Gao Ming , Wan Jinzhao , Du Yiming , Zhao Shiguang
Published: April 2020

Background

Medulloblastoma (MB) is the most common intracranial malignant tumor in children. The genes and pathways involved in the pathogenesis of MB are relatively unknown. We aimed to identify potential biomarkers and small-molecule drugs for MB.

Methods

Gene expression profile data sets were obtained from the Gene Expression Omnibus (GEO) database and the differentially expressed genes (DEGs) were identified using the Limma package in R. Functional annotation, and cell signaling pathway analysis of DEGs was carried out using DAVID and Kobas. A protein-protein interaction network was generated using STRING. Potential small-molecule drugs were identified using CMap.

Result

We identified 104 DEGs (29 upregulated; 75 downregulated). Gene ontology analysis showed enrichment in the mitotic cell cycle, cell cycle, spindle, and DNA binding. Cell signaling pathway analysis identified cell cycle, HIF-1 signaling pathway, and phospholipase D signaling pathway as key pathways. SYN1, CNTN2, FAIM2, MT3, and SH3GL2 were the prominent hub genes and their expression level were verified by RT-qPCR. Vorinostat, resveratrol, trichostatin A, pyrvinium, and prochlorperazine were identified as potential drugs for MB. The five hub genes may be targets for diagnosis and treatment of MB, and the small-molecule compounds are promising drugs for effective treatment of MB.

Conclusion

In this study we obtained five hub genes of MB, SYN1, CNTN2, FAIM2, MT3, and SH3GL2 were confirmed as hub genes. Meanwhile, Vorinostat, resveratrol, trichostatin A, pyrvinium, and prochlorperazine were identified as potential drugs for MB.
The prognostic value of preoperative serum CA724 for CEA-normal colorectal cancer patients
Authors: Kuang Jiaan , Gong Yizhen , Xie Hailun , Yan Ling , Huang Shizhen , Gao Feng , Tang Shuangyi , Gan Jialiang
Published: April 2020

Background and Purpose

There had been no recognized serum tumor marker to predict the prognosis of colorectal cancer (CRC) patients with normal preoperative serum carcinoembryonic antigen (CEA) levels. The purpose of this study was to determine whether preoperative serum carbohydrate antigen 724 (CA724) was of predictive function for the prognosis of CRC patients with normal CEA levels.

Methods

The medical records of 295 CRC patients with normal CEA levels who underwent surgery at the Department of Colorectal Anal Surgery of the First Affiliated Hospital of Guangxi Medical University (Guangxi, China) between September 2012 and September 2014 were retrospectively reviewed. The Chi-square test was used to test the correlation between preoperative serum CA724 levels and clinical features. Kaplan–Meier curves were conducted to calculate the overall survival (OS) rate and disease-free survival (DFS) of patients. Cox regression analysis was applied to conduct univariate and multivariate analysis of the following four preoperative serum tumor makers namely CA724, carbohydrate antigen 199 (CA199), carcinoembryonic antigen 125 (CA125), carcinoembryonic antigen 242 (CA242) and clinical features. Nomograms for prognostic parameter of OS and DFS were developed using R v3.2.5.

Results

In the Chi-square test, only pathological node stage (pN stage) (X2= 14.514,P= 0.001) and differentiation (X2= 10.712,P= 0.001) were associated with serum CA724 levels. In the Kaplan–Meier analysis, the results revealed that the OS and DFS in patients with high CA724 was poorer than those with normal. In the multivariate Cox regression analysis of OS and DFS, only pT stage, pN stage, metastasis and serum CA724 were independent prognostic risk factors for CRC patients with normal CEA levels.

Conclusion

Preoperative serum CA724 might serve as a potential prognostic factor for CRC patients with normal serum CEA levels.
Impact Assessment of Goods and Services Tax (GST) on Shopkeepers
Authors: Semwal Shatakshi , Rani Ella , Verma Vandana
Published: April 2020

India has got a well-structured and simplified taxation system, where authoritative segregation has been done among the Central government, state governments and local bodies. But with the implementation of GST, the harmonization of tax rules, regulations, rates, processes and procedures across the states was expected to improve the ease of doing business in India. The removal of cascading of taxes and transaction costs associated with inter-state transactions was also expected to encourage investment and therefore, the economic growth of country. With the objective of the impact assessment of Goods and Services Tax on shopkeepers the present study was carried out. The study was conducted in Hisar city of Haryana state where the findings highlighted that shopkeepers had highly increased tax burden and legal compliances, followed by increase in workers hiring capacity and no impact on ease of doing business, no loss and transparency in business. Further, results also showed that there was decrease in profit as they have to pay taxes more than 6 times in a year. With the implementation of GST, shopkeepers were having constraint in filing GST as now is getting a complicated process and due to changing GST rates they were facing high competition amongst the market.
Influence of Scale of Operation and Farmers’ Risk Aversion on Sugarcane Productivity in Nandi County, Kenya
Authors: Cheruiyot Joseph Kipkorir , Sang Nelly
Published: April 2020

Aims: Sugarcane ranks among top ten commercial crops grown in Kenya, but its productivity has been on the decline. This study investigated influence of scale of farm operation and farmers’ risk aversion on productivity. Risk aversion was based on farmers’ perceived risks associated with new high yielding, early maturing varieties.

Study Design: The survey study adopted an ex post facto research design.

Place and Duration of Study: The study was carried out in Nandi County, Western parts of Kenya along a sugarcane growing belt. Data was collected between April and September, 2019.

Methodology: An enumerator-administered questionnaire was used to collect data from a sample of 198 respondents. Purposive and stratified random sampling techniques were used to select participants. Data was analyzed with the aid of SPSS Version 20. Chi square test and its related measure of strength of association; Cramer’s V, were utilized to estimate relationships between variables. Welch’s ANOVA (W-test) was run to test for yield differences between groups. Significant differences were subjected to post hoc tests using Games-Howell test to separate the means.

Results: There was a significant association between farmers scale of operation and productivity;