bluuebunny/crossref_metadata_2025 huggingface dataset:
History of the Bantu Peoples by Credo Mutwa– Zulu Tradition or Mutwa’s Invention?
Authors: Podolecka Agnieszka
Published: December 2023

Vusamazulu Credo Mutwa (1921–2020) was one the most famous and controversial sangomas – sages, clairvoyants, healers and diviners – in South Africa. He was also the first sangoma who published books revealing sangomas’ esoteric calling, vocation and cooperation with spirits. He wrote extensively about Zulu mythology. A part of his version of mythology is the ‘history’ of the Bantu languages speaking peoples, their divine origins and the conquest of the lands in the South of Africa. Mutwa also wrote about relationships between Zulus and other ethnic groups, focusing on their mythological beginnings. The aim of this article is to analyse Mutwa’s myths and stories about the history of Bantu languages speaking peoples, relationships between Zulus and other peoples, and place it all within historical facts and religious beliefs known to academics. The article is part of the project ‘Esoteric landscape of Southern Africa’ financed by the Polish National Science Centre (Narodowe Centrum Nauki), Poland, project no. 2017/25/N/HS1/02500.
Dark Side of Institutional Entrepreneurship in Latin America: Vistas from Reflexivity
Authors: Méndez Oscar Javier Montiel , García Rosa Azalea Canales , Novelo Anel Flores
Published: December 2023

Entrepreneurial development in Latin America is varied and complex. The concept of reflexivity can be used to investigate the complexities of the entrepreneurial system in Latin America; however, it has rarely been used in entrepreneurship, which refers to finding strategies to question our attitudes, thought processes, values, assumptions, prejudices, habitual actions, and understanding how we relate to others. To explore reflexivity and how this area can provide support to reimagine Latin American entrepreneurial dynamics, a literature review was conducted, and two theoretical models were proposed that show the complexity of the region and routes where reflexivity can foster a path for the region to change and advance its entrepreneurial efforts.
Intensity of Physical Activity and Quality of Life Among Older Adults
Authors: Kuska Michalina , Nowak Maria A. , Żukowska Hanna , Pasek Marcin , Szark-Eckardt Mirosława
Published: December 2023

Introduction.
Low physical activity, transition to retirement and unfavorable socio-economic changes may significantly reduce the quality of life of older people. The increasing average life expectancy of the population requires measures intended to promote physical activity among the elderly in order to improve their quality of life. The aim of the research was to determine the relationship between exercise intensity and the quality of life in U3A students over 60 years of age.

Material and methods.
A diagnostic survey was applied in the research. The following research tools were used: WHOQOL-BREF Questionnaire to assess the quality of life, Minnesota Questionnaire (MLTPAQ) to test the intensity level of physical activity, and the authors’ own questionnaire. The study involved 673 individuals over 60 years of age from the Kuyavian-Pomeranian Region in Poland.

Results.
People who took low-intensity exercise more often were more satisfied with life and health. Likewise, they had higher quality of life assessments in the physical, psychological and environmental context in comparison with those who were not sporty. Individuals who went in for moderate physical effort more often assessed their quality of life better in the social area. Respondents who engaged in intense physical activities valued the quality of life in physical, psychological and environmental domains higher.

Conclusions.
The positive influence of regular exercise on the quality of life requires further promotion of this activity, health education, and extension, diversification and adjustment of the offer of sports activities to the needs and capabilities of the elderly.
Analysis of the Indoor Climate in a Small, Newly-Built Auditorium
Authors: Budiaková Mária
Published: December 2023

The article focuses on an analysis of the indoor climate in a small, newly-built auditorium. Providing optimal indoor climate parameters in the interiors of a university is extremely important for students. Meeting these parameters is essential not only from a physiological point of view, but also to achieve desirable student performances. The parameters of CO2 concentration are also influenced by the air distribution system in a small auditorium. The correct positions of the supply and extract air is very important. The first experimental measurements of CO2 concentration were carried out in a small auditorium in an old building. The natural ventilation of the small auditorium was evaluated. The second experimental measurements of the CO2 concentration were made in a small auditorium in a newly-built building. The ventilation system and the modern air distribution system of the small, newly-built auditorium were evaluated based on the CO2 concentration and the PMV index. A Testo 480 was used for the measurements. The conclusion of this paper contains the recommendations for the design of a newly-built ventilation system in a small auditorium.
Legal Strategies and Challenges in Climate Change Mitigation in Vietnam: The Role of Non-Contractual Damages Compensation
Authors: Thien Nguyen Phuc
Published: December 2023

Vietnam is exploring numerous strategies to combat climate change, with a growing emphasis on enhancing national legal provisions, particularly those related to non-contractual environmental damage compensation. However, it is a matter of debate whether Vietnam’s current regulations on non-contractual damages effectively contribute to climate change mitigation. This article highlights both the impact and limitations of existing laws concerning environmental non-contractual damages. It argues for a legal approach that prioritizes preventing wrongful environmental acts over merely addressing their consequences. This article is structured into four parts: (i) An overview of climate change in Vietnam; (ii) Comparative analysis of non-contractual compensation for climate-related damages across various jurisdictions, (iii) An examination of Vietnam’s legal framework for non-contractual compensation due to environmental pollution; and (iv) Conclusions and recommendations.
Bibliometric Study of Life Cycle Analysis Applied to Motor Vehicles
Authors: Juvêncio Jardel de Queiroz , Da Silva William Neves , Freire Francisco Nivaldo Aguiar
Published: December 2023

Purpose: Conducting a bibliometric analysis of papers on the Life Cycle Assessment (LCA) of motor vehicles published over the last 20 years.
 
Theoretical framework: Bibliometric analysis, a discipline within scientometrics, uses mathematical and statistical methods to synthesize and evaluate publications, identifying trends and structures in specific research fields.
 
Method: The bibliometric analysis was conducted using data from Scopus and Web of Science, and it assessed the impact and trends of scientific production, correlating keywords to explore connections between themes. Articles from the last two decades were analyzed to quantify the relevance of the studied topic.
 
Results and conclusion: Using the Bibliometrix algorithm in R, 512 published articles were analyzed. The results indicated a significant increase in research output in this field of study, as demonstrated by the substantial growth in the number of published articles and citations.
 
Research implications: This bibliometric study reveals key trends and guides future innovations in the environmental and transportation sectors, highlighting the transition towards more sustainable options.
 
Originality/value: This groundbreaking work provides valuable insights into the life cycle of vehicles with various propulsion technologies, emphasizing the evolution and environmental impacts of electric, hybrid, internal combustion, and fuel cell vehicles.
ANALISIS PEMBANGUNAN KAYUTANGAN HERITAGE DARI FORMULASI SAMPAI DENGAN EVALUASI KEBIJAKAN, SERTA DAMPAKNYA TERHADAP MASYARAKAT KOTA MALANG
Authors: Sekarsari Retno Wulan , Rachmatullah Putra Langgeng
Published: December 2023

Abstrak

 

Tujuan dilaksanakannnya penelitian ini adalah untuk mengetahui dan menganalisa optimalisasi dari diterapkanya Kebijakan Publik di Kota Malang. Identifikasi masalah dalam penelitian ini adalah tentang kepuasaan, respon, dan dampak dari ditetapkanya Kebijakan Kayu Tangan Heritage di Kota Malang. Peneliti menggunakan teori Kebijakan Publik dengan teori pendukung sebagai berikut: Implementasi, Formulasi, dan Evaluasi Kebijakan, dan Komunikasi Kebijakan. Peneliti juga mengggunakan teori komunikasi yang didalamnya memuat : Prinsip Komunikasi yang efektif. Selain itu penerapan teori Perencanaan Pembangunan juga digunakan dipenelitian ini. Semua teori tersebut sangat dibutuhkan untuk menganalisis pembangunan Kayutangan Heritage dalam menetukan dukungan, respon, dan dampaknya dimasyarakat. Metodologi yang digunaan adalah metode campuran yang terfokus untuk menganalisis dan evaluasi Kebijakan Kayutangan Heritage di Kota Malang dan peningkatan komunikasi publik Pemerintah Kota Malang terkait program yang ada di Kayutangan Heritage. Hasil dari penelitian ini adalah indeks kepercayaan, dukungan, dan partisipasi masyarakat yang menunjukkan huruf A dengan predikat sangat baik, selain itu dari segi dampak pembangunan Kayutangan Heritage menunjukkan huruf B dengan predikat baik dalam untuk dijalankan di kehidupan masyarakat. Komunikasi Pemerintah Kota Malang dalam mewadahi dan mengetahui dukungan, dan penyebaran informasi masyarakat atas pembangunan Kayutangan Heritage, dengan memanfaatkan media sosial khususnya twitter dan kompas yang memiliki jaringan yang lebih luas. Tetapi pada pengelolaan dibidang keamanan dan sosialisasi dalam membina partnership antar sektor masih sangat lemah dan belum dilakukan secara optimal.

Kata Kunci: Optimalisasi, Evaluasi, Kebijakan Publik.
A criação da Escola Doméstica de Nossa Senhora do Amparo para a formação de mulheres
Authors: Tavares Micheli da Cruz Cardoso , Vasconcelos Maria Celi Chaves
Published: December 2023

O artigo resulta da investigação sobre a Escola Doméstica de Nossa Senhora do Amparo, fundada em janeiro de 1871, na cidade de Petrópolis, que tinha como premissa atender a meninas pobres, órfãs e desvalidas. O objetivo geral é analisar as expectativas da formação confessional feminina para a infância desvalida, moldada no interior daquela instituição. Em um plano mais específico, o estudo propõe-se a investigar a educação feminina católica e suas concepções transmitidas desde a infância; as práticas pedagógicas educativas produzidas no interior da escola; e a cultura material escolar católica como uma construção que se manifesta de diferentes formas. Trata-se de uma pesquisa histórica, cujo corpus documental é constituído, sobretudo, pelo acervo existente no interior da escola, em um arquivo intitulado “Sala-Museu”. Assim, o estudo configura-se em uma contribuição para a história das mulheres das camadas mais desfavorecidas da população, cujos registros de suas práticas educacionais ainda se encontram silenciados.
Memórias e trajetórias femininas no âmbito dos Centros Integrados de Educação Pública
Authors: Faria Lia Ciomar Macedo de , Aguiar Alexsandra Aparecida Silva do Prado de
Published: December 2023

O presente artigo objetiva fazer uma análise da implantação dos Centros Integrados de Educação Pública durante os dois mandatos de Leonel Brizola como governador do estado do Rio de Janeiro. Buscou-se refletir sobre o projeto a partir das memórias e narrativas de duas ex-diretoras sobre suas trajetórias familiares, escolar e profissional nos Centros Integrados de Educação Pública 123 (Nova Friburgo) e 297 (Vassouras). Optou-se por utilizar como ferramenta metodológica para este trabalho entrevistas semiestruturadas baseadas na história oral. Do ponto de vista teórico, empregou-se, com base nos estudos de Pierre Bourdieu, o conceito de habitus, com vistas à apreensão das trajetórias familiares, escolares e profissionais das professoras. Para fundamentar o trabalho de memória, o estudo se baseou principalmente nas formulações de Walter Benjamin e Paul Thompson para qualificar a abordagem de memória, destacando-se pela interconexão entre história e experiência pessoal.
Severe hypercalcemia induced by iatrogenic hypervitaminosis d: case report
Authors: Amaral Maria Eduarda , Kotsifas Sofia Alessandra , Cornelsen Luisa Aparecida , Cruz Aristides
Published: December 2023

INTRODUCTION: Vitamin D intoxication among nurslings (serum levels of 25-hydroxyvitamin D above 150 ng/mL) is a factor in the development of hypercalcemia, an uncommon condition in pediatrics. OBJECTIVE: Report a case of severe hypercalcemia caused by hypervitaminosis D in a nursling. CASE REPORT: 5-months-old masculine child, exclusively breastfed, referred to a specialist with suspected cows milk protein allergy because he failed to gain weight in the last month and presented with blood and mucus in stool. Beyond failure to thrive, the patient evolved with somnolence, apathy and hypotonia. Laboratorial tests revealed hypercalcemia (serum calcium of 17,40 mg/dL) and hypervitaminosis D (180 ng/mL). The hypothesis of vitamin D intoxication was raised and later confirmed by the laboratorial analysis of the supplement offered to the nursling, which revealed a vitamin D concentration 46 times greater than the originally prescribed by the pediatrician. CONCLUSION: Due to the currently high diagnostic suspicion of cows milk protein allergy in nurslings, other causes of these symptoms cant be neglected in the investigation when the elimination diet fails. In this nursling, the persistence of failure to thrive, apathy, anorexia and hypotonia during four months motivated the investigation that showed a severe hypercalcemia due to the usage of manipulated vitamin D.
NEUROTOXIC EFFECT OF ZINC IN GLAUCOMA: ROLE OF PIGMENT EPITHELIUM-DERIVED FACTOR
Authors:   Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Bulgakov T.K. , Shebardina N.G. , Zernii E.Yu. , Vologzhannikova A.A. , Shevelyova M.P. , Baksheeva V.E. ,   Institute for Biological Instrumentation RAS, Pushchino,   Institut Neurophysiopathologie, INP, Faculté des Sciences Médicales et Paramédicales, Aix Marseille Univ, Marseille, France
Published: December 2023

Recent studies have demonstrated that retinal ganglion cell (RGC) death in glaucoma may be associated with an acute increase of mobile zinc. Our work is devoted to the identification and characterization of secreted Zn2+-binding retinal signal proteins, which may mediate cytotoxic effects of zinc.
INDUCTION OF IMMUNOLOGICAL TOLERANCE TO GRAFT ALLOANTIGENS IN SKIN FLAP MODEL USING TRANSFECTED DENDRITIC CELLS AND AN INHIBITOR OF CYCLIN-DEPENDENT KINASES 8/19
Authors:   Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Bulygin A.S. , Fisher M.S. , Kurilin V.V. , Khantakova Yu.N. , Sennikov C.V.
Published: December 2023

The induction of transplantation tolerance through the induction of donor-specific T cells with tolerogenic DCs and ar-Tregs may provide additional opportunities to overcome the problem of organ rejection during transplantation. With the introduction of DC transfected with DNA constructs and anti-Treg obtained by stimulation with AS2863619, the prolongation of the rejection of the transplanted skin flap increased compared to the control group.
STUDY OF MECHANISMS OF ACTIVATION OF THE INSULIN-LIKE GROWTH FACTOR RECEPTOR (IGF-IR) USING MUTANT FORMS OF THE RECEPTOR
Authors:   Moscow Institute of Physics and Technology, Dolgoprudny, Gavrilenkova A.A. , Deev I.E. , Bocharov E.V. , Okhrimenko I.S. , Serova O.V. ,   Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow
Published: December 2023

At the moment, a lot of research is being conducted to study the mechanisms of activation of the insulin receptor family, but the exact mechanisms of conformational changes in receptors during activation are still being investigated. It is assumed that in the inactive state, the TM domains of the insulin receptor are in a conformation that prevents the interaction of kinase domains. Binding to the ligand changes the conformation of the receptor, as a result, intracellular tyrosine kinase domains converge and cause a cellular response.
ALTERATION OF THE TRANSCRIPTOME OF GENETICALLY MODIFIED BREAST CANCER LINES
Authors:   Cancer Research Institute, Tomsk, Gaptulbarova K.A. , Nevskaya K.V. , Tsydenova D.S. , Dolgasheva D.S. , Pershina A.G. , Litviakov N.V. ,   Tomsk State University,   Siberian State Medical University, Tomsk
Published: December 2023

We have previously shown an association of the ability of tumour cells to dedifferentiate to stem cells depending on the number of stemness gene amplifications that account for their overexpression. The breast cancer cell line BT549 has a single KLF5 stemness gene amplification and the lowest ability to dedifferentiate to form mammospheres of any standard breast tumour line. In due course, the MDA-MB231 cell line was characterised by the presence of MYC gene amplifications and a high ability to dedifferentiate to form mammospheres. Using CRISPER/Cas9 method, genetically modified BT549 and MDA-MB231 cell lines with high and low MYC gene expression, respectively, were obtained.
EX VIVO CHARACTERISTICS OF NON-SMALL CELL LUNG CANCER CELLS
Authors:   Novosibirsk State University, Gileva M.S. , Ufimceva E.G. , Gulyaeva L.F. , Kozlov V.V. ,   Federal Research Center for Fundamental and Translational Medicine, Novosibirsk,   Institute of Molecular Biology and Biophysics, Novosibirsk,   Novosibirsk Regional Clinical Oncological Dispensary
Published: December 2023

The search for molecular markers for the identification of adenocarcinoma and squamous cell lung cancer remains an urgent problem. In this work, for the first time, an ex vivo method was used to isolate cells from tissue samples of patients with non-small cell lung cancer. Potential markers of this histological tumor type were analyzed, and the cellular composition of the immune microenvironment was also assessed, including depending on the smoking status of patients.
PEPTIDE CONJUGATES OF RNASE P INHIBITING OLIGONUCLEOTIDES: SYNTHESIS AND PROPERTIES
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Danilin N.A. , Matveev A.L. , Bardasheva A.V. , Golyshev V.M. , Novopashina D.S. ,   Novosibisk State University
Published: December 2023

Peptide conjugates of inhibiting bacterial RNase P oligonucleotides were obtained using optimized approaches. The influence of peptides on inhibiting oligonucleotide – RNA-target complex stability, inhibition of RNase P by synthesized conjugates, efficacy of penetration in bacterium A.baumannii and E.coli and their antibacterial activity was investigated.
DERIVATION AND APPLICATION OF AIRWAY BASAL CELLS FROM HUMAN-INDUCED PLURIPOTENT STEM CELLS FROM CYSTIC FIBROSIS PATIENTS
Authors:   Research Centre for Medical Genetics, Moscow, Demchenko A.G. , Belova L.L. , Balyasin M.V. , Kondrateva E.V. , Lavrov A.V. , Smirnikhina S.A. ,   Peoples’ Friendship University of Russia, Moscow
Published: December 2023

Airway basal cells are tissue-specific progenitor epithelial cells capable of self-renewal and differentiation into specialized lung cells. We have developed a protocol for obtaining basal cells from induced-pluripotent stem cells from patients with cystic fibrosis and a healthy donor. The conditions for non-viral and viral delivery of transgenes to basal cells were selected. Also functional assessment of the CFTR channel conductance in lung organoids derived from basal cells was carried out.
РАЗРАБОТКА МЕТОДА ДОСТАВКИ И ВИЗУАЛИЗАЦИИ HIF1-Α В КЛЕТКАХ ГЛИОМЫ ЧЕЛОВЕКА U-87 MG
Authors:   Institute of Life Sciences and Biomedicine, Far Eastern Federal University, Vladivostok, Doroshenko V.O. , Goncharov N.V.
Published: December 2023

We have developed and constructed a recombinant construction that contains the ODD domain associated with the fluorescent reporter mCherry. Compounds capable of inhibiting HIF-1, PHD, VHL or other molecular determinants of the hypoxic response of tumors will lead to a loss or increase in the fluorescent signal in cells. Candidates detected using this method can be considered as possible antitumor drugs.
MICRORNA OF SERUM EXTRACELLULAR VESICLES AS MARKERS OF TYPE 2 DIABETES MELLITUS DEVELOPMENT IN OBESITY
Authors:   Petersburg Nuclear Physics Institute named after B.P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Dracheva K.V. , Pobozheva I.A. , Anisimova K.A. , Hamid Z.M. , Balandov S.G. , Vasilevsky D.I. , Pchelina S.N. , Miroshnikova V.V. ,   Pavlov First Saint Petersburg State Medical University
Published: December 2023

The study involved microRNA profiling of extracellular vesicles (EV) secreted by adipose tissue in obese patients and in a control group, followed by content analysis of microRNAs demonstrating differences in adipose tissue EV, in serum EV in obese patients with/without T2DM and in the control group. We found 2 microRNAs: hsa-miR-551b-3p and hsa-miR-302d-3p, which may be markers of T2DM development in obesity.
TISSUE-SPECIFIC KNOCKDOWN OF OVERLAPPING LAWC AND TRF2 GENES IN SOMATIC AND REPRODUCTIVE TISSUES OF DROSOPHILA MELANOGASTER
Authors:   Koltzov Institute of Developmental Biology RAS, Moscow, Zavoloka E.L. , Vorontsova Yu.E. , Tcherezov R.O. , Simonova O.B.
Published: December 2023

Overlapping genes have been found in all model eukaryotic species. However, the regulation of their expression and the mechanisms of occurrence of intergene overlaps have not been studied enough. In this work, we’ve researched the expression of two overlapping genes, lawc and Trf2, in the D. melanogaster genome by knockdown of bidirectional transcripts of these genes in vivo. We demonstrated that antisense lawc transcripts may be involved in the control and coordination of the expression of Trf2 sense transcripts.
STUDY OF THE MECHANISMS OF AUTO-AGGREGATION OF ENTEROPATHOGENIC ESCHERICHIA COLI O157:H7 USING A SYSTEM BASED ON MAGNETIC LEVITATION
Authors:   Gamaleya National Research Centre for Epidemiology and Microbiology, Moscow, Zakharchenko A.E. , Domnin P.A. , Ermolaeva S.A.
Published: December 2023

The paper compares the autoaggregation ability of the enteropathogenic bacterium Escherichia coli O157:H7 and its transcription factor RcsB mutant using a new system based on magnetic levitation. It has been found that the transcription factor RcsB affects the process of autoaggregation through the control of curli-fimbria.
USING OF RHT (-B1A, -B1B, D1A, D1B) SEMI-DWARFISM GENES AND COARSE-GRAINED TAGW8-B1A, TAGS 5-3A GENES FOR INCREASING THE PRODUCTIVITY OF SPRING WHEAT
Authors:   S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan, Zotova L. , Zhumalin A. , Gajimuradova A. , Zhirnova I. , Abdulloyеv F. , Nuralov A.
Published: December 2023

To speed up the selection process of hybrids and the selection of parental forms for crosses, it is important to pay attention to both the phenotypic and genetic potential of plants. In order to increase yields by selecting optimal parameters for wheat selection based on short-stemmed and coarse-grained characteristics in a dry climate, we studied the genes of the “green revolution” group Rht and coarse-grained TaGW, TaGS, and carried out a correlation analysis between the genetic potential of wheat varieties and hybrids and their phenotypic manifestation in drought conditions. The results of the studies showed that the Rht-D1b gene significantly reduces the height of plants by 51.67 %, and also reduces the length of the coleoptile by 30.45 %. At the same time, the TaGW8-B1a gene increases the grain width by 13.37 %. However, in the presence of Rht-D1b, Rht-B1b genes in the genome, the average weight of 1000 seeds was 35.06 g (p<0.05), while the Rht-B1b gene increased it by 9 % (p<0.05), both Rht-D1a, Rht-B1a alleles give a mass of 1000 seeds of 37.96 g (p<0.05). The data obtained indicate the relationship of phenotypic and genetic parameters in wheat between the genes of coarse-grained and short-stemmed, which makes it possible to select based on the genetic parameters of varieties and hybrids, thereby speeding up the selection process.
A NOVEL DNAZYME-BASED FLUORESCENT BIOSENSOR FOR DETECTION OF RNA-CONTAINING SABIA MAMMARENAVIRUS
Authors:   Pasteur Institute of Epidemiology and Microbiology, Saint Petersburg, Kirichenko A.D. , Bryushkova E.A. , Dolgova A.S. , Dedkov V.G. ,   Faculty of Molecular Biology, Lomonosov Moscow State University
Published: December 2023

Sabia virus, which causes Brazilian hemorrhagic fever (BHF), is a poorly studied Arenavirus. Existing laboratory diagnostic methods for BHF (ELISA, electron microscopy, immunohistochemistry, RT-PCR) require expensive equipment and are labor-intensive. There is a need to develop simple, rapid, and accurate test systems for virus detection in field conditions, improving diagnostic accessibility and facilitating the work of healthcare professionals and researchers.
CHANGES IN GUT MICROBIOTA ASSOCIATED WITH SMOKING AND SMOKING CESSATION
Authors:   Kazan Federal University, Kovalenko A.V. , Boulygina E.A. , Senina A.M. , Siniagina M.N. , Khusnutdinova D.R. , Markelova M.I. , Abdulkhakov S.R. , Grigoryeva T.V.
Published: December 2023

This study used next-generation sequencing to characterize changes in the human gut microbiome under the influence of cigarette smoking and smoking cessation. Five days after smoking cessation, the composition of the gut microbiota tends to return to an unaffected state.
ANALYSIS OF THE EFFICIENCY OF MIRNA DELIVERY USING NANOPARTICLES IN EX VIVO TISSUE SLICES OF THE LIVER
Authors:   Privolzhsky Research Medical University, Nizhny Novgorod, Kozlov D.S. , Rodimova S.A. , Krylov D.P. , Mozherov A.M. , Zyuzin M.V. , Kuznetsova D.S. ,   Lobachevsky State University of Nizhny Novgorod,   ITMO University, Saint Petersburg
Published: December 2023

The study is aimed at selecting the optimal carrier for miRNA delivery. In the course of the study, miRNAs targeting insulin-like growth factor 1 were selected. The distribution and cytotoxicity of several types of nanoparticles were analyzed using a model of liver tissue explants and multiphoton microscopy. The efficiency of suppression of IGF1 expression was assessed using stem-loop PCR, RT-PCR and ELISA.
CYTOSTATIC EFFECT OF COLD PLASMA JET ON HUMAN TUMOR CELLS
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Kryachkova N.V. , Polyakova A.A. , Biryukov M.M. , Milakhina E.V. , Zakrevsky D.E. , Schweigert I.V. , Koval O.A. ,   Novosibirsk State University,   Khristianovich Institute of Theoretical and Applied Mechanics SB RAS, Novosibirsk
Published: December 2023

Cold plasma activates various processes when acting on biomolecules, including tumors. Сold plasma jet (CPJ) generates active forms of oxygen and nitrogen, and such species cause oxidative stress. This study shows an increase in active forms of nitrogen in irradiated A549 tumor cells compared to healthy Wi-38 cells. The action of CPJ on tumor cells causes cell cycle arrest, and suppresses their proliferation.
FEATURES OF THE GLUTATHIONE REDOX SYSTEM IN PATIENTS AFTER CORONAVIRUS INFECTION
Authors:   Academy of Biology and Biotechnology named after D.I. Ivanovsky, SFedU, Rostov-on-Don, Kulikova D.B. , Nosova V.O. , Tishchenko A.A.
Published: December 2023

COVID-19 includes a wide range of clinical signs and symptoms ranging from asymptomatic infections to acute respiratory distress. The glutathione redox system, represented in the human body by the enzymes glutathione-S-transferase (GST), glutathione peroxidase (GPO), and glutathione reductase (GR), provides antioxidant protection for cells. Oxidative stress plays a significant role in the course of coronavirus infection, therefore, an assessment of the state of the glutathione redox system allows us to draw conclusions about the presence and severity of oxidative stress in sick patients. In addition, studies of the antioxidant role of the glutathione redox system in protecting cells from oxidative stress have a perspective in the development and implementation of various pharmaceutical products.
ESTIMATION OF DONOR CHIMERISM AFTER TRANSPLANTATION OF HUMAN CD34+ HEMATOPOIETIC STEM CELLS TO NBSGW MICE BY DIGITAL DROPLET PCR
Authors:   R.M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Saint Petersburg, Laushkina V.O. , Shakirova A.I. , Komarova Y.V. , Muslimov A.R. , Onopchenko A.V. , Shchelina E.V. , Ostrova S.A. , Vasutina M.G. , Lepik K.V. ,   V.A. Almazov National Medical Research Center of the Ministry of Health of the Russian Federation, Saint Petersburg
Published: December 2023

In preclinical studies, there is a problem of estimating donor chimerism in animal models engrafted with human CD34+ cells. Using digital droplet PCR (ddPCR) more accurate results can be achieved in comparison with routine flow cytometry. The aim of the study was to develop a protocol for determining the level of chimerism by ddPCR in the bone marrow of NBSGW mice.
THE ACTIVITY OF HUMAN NK CELLS TOWARDS HETEROTYPIC CELLULAR 3D MODEL OF BREAST CANCER
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Leonteva A.A. , Abdurakhmanova M.M. , Urina A.A. , Kulemzin S.V. , Richter V.A. , Kuligina E.V. , Nushtaeva A.A. ,   Novosibirsk State University,   Institute of Molecular and Cellular Biology SB RAS, Novosibirsk
Published: December 2023

More advanced cellular models that adequately reflect the heterogeneity of the breast cancer (BC) should foster the development of new treatments. Heterotypic spheroid (3D) models that consist of histologically distinct cell populations can mimic heterogeneity of BC. In this work, we selected the conditions for the formation of such models, and evaluated the effect of IL-15 and TGFβ cytokines on the infiltration and killer activity of NK cells in a 3D model.
ASSOCIATION BETWEEN HIGH CONCENTRATIONS OF CIRCULATING CELL-FREE DNA AND SUICIDE ATTEMPTS IN SCHIZOPHRENIA
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Melamud M.M. , Ermakov E.A. , Brit P.I. , Zhuravlev E.S. , Balakhonova E.A. , Stepanov G.A. , Kamaeva D.A. , Ivanova S.A. , Nevinsky G.A. , Buneva V.N. ,   Mental Health Research Institute, Tomsk National Research Medical Center RAS
Published: December 2023

The paper presents the results of measuring the concentration of total, nuclear and mitochondrial circulating cell-free DNA in blood plasma in schizophrenia. It has been shown that the concentration of total and nuclear, but not mitochondrial cell-free DNA, is significantly higher in patients with a history of suicide attempts compared to patients without suicide attempts and healthy individuals without mental pathology
ANTIBODY ACTIVITY IN MICRORNA HYDROLYSIS: ROLE IN GENE REGULATION IN VIRAL INFECTIONS
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Nikitin A.O. , Timofeeva A.M. , Sedykh S.E. , Nevinsky G.A. ,   Novosibirsk State University
Published: December 2023

Antibodies with catalytic activity, hydrolyzing DNA, RNA and some oligopeptides have been described in a number of viral and autoimmune diseases. Antibodies with RNA-hydrolyzing activity can be important in the degradation of microRNAs and, as a consequence, in the change in the regulation of genes carried out with the help of microRNAs. The aim of this work is to investigate the activity of antibodies of HIV-infected patients in microRNA hydrolysis.
ESTIMATING FUNCTIONAL STATE OF THE BLOOD-RETINAL AND HIPPOCAMPAL BLOOD-BRAIN BARRIERS IN OXYS RATS ALONG THE DEVELOPMENT OF AGE-RELATED MACULAR DEGENERATION AND ALZHEIMER’S DISEASE FEATURES
Authors:   Institute of Cytology and Genetics SB RAS, Novosibirsk, Peunov D.A. , Telegina D.V. , Kozhevnikova O.S. , Kolosova N.G.
Published: December 2023

Age-associated macular degeneration and Alzheimer’s disease are neurodegenerative diseases with debatable ethiology. Disfunction of the blood-retinal and blood-brain barriers, which are formed by tight junctions, may be essential to the development of the pathologies. This work is dedicated to the estimation of blood-retinal and hippocampal blood-brain barriers’ permeability and functional state, so as the content of tight junctional proteins.
THE EFFICIENCY OF LISTERIA MONOCYTOGENES INVASION INTO CATTLE CELLS DEPENDS ON PHYLOGENETICALLY DETERMINED ISOFORMS OF PATHOGENICITY FACTORS INLA AND INLB
Authors:   Gamaleya National Research Centre for Epidemiology and Microbiology, Moscow, Safarova P.V. , Chalenko Y.M. , Fedorova V.A. , Zakharchenko A.E. , Ermolaeva S.A. ,   Pirogov Russian National Research Medical University, Moscow
Published: December 2023

The aim of the research is to compare the invasion efficiency of isogenic recombinant strains of L. monocytogenes, which differ only in allelic variants of the inlA and inlB genes, in bovine cell lines.
A patient with severe acne: therapy perspectives
Authors: Trapeznikova Tatiana V. , Letyaeva Olga I. , Ziganshin Oleg R. , Gubina Valeria O.
Published: December 2023

Acne is a common dermatological disease that peaks between 12 and 25. The tendency of inflammatory elements to appear on the skin, especially the face, leads to psycho-emotional imbalance and significantly reduces the patient’s quality of life. In 95% of cases, the outcome of acne is the formation of cicatricial deformities, the treatment of which often takes years. If inflammatory elements are present, treatment should be aimed at ensuring a long-term remission of the disease, including preventing the formation of new scars.
This work aims to determine the efficacy and safety of acne treatment using systemic isotretinoin.
The article presents the results of systemic isotretinoin use in severe forms of acne and acne that is resistant to standard treatments based on clinical cases. It was proved that the benefits obtained from isotretinoin usage are much more significant than the possible risk of side effects, especially if the patient’s skin tends to scar. The latest data on the etiology and pathogenesis of cicatricial deformities is presented. A review of modern techniques for the correction of post-acne cicatricial deformities was carried out.
Isotretinoin is clinically proven to be highly effective and safety safe for both severe acne.
The use of isotretinoin to treat acne is effective and reduces the risk of scarring.
Azerbaijan and the Rise of Turkic Unity: The Journey of the Organization of Turkic States
Authors: Shafiyev Farid
Published: December 2023

This commentary delves into the evolution of the Organization of Turkic States (OTS), highlighting its transformation from a consultative mechanism to a robust international entity with geostrategic significance. The paper examines key developments such as the Shusha Declaration, the impact of the Second Karabakh War, and the strategic partnership among Turkic states in the context of global geopolitical dynamics. The paper focuses on Azerbaijan’s vision of the development of the Turkic unity, some historical aspects, which preceded the idea of the Turkic unity, and contemporary trends in the regional and global politics, which gave impetus to the enhancement of the OTS. The conclusion highlights the importance of Turkic unity as it stands out more prominently in the complex geopolitical environment and posits that trends make it necessary to strengthen and develop cooperation among the Turkic states.
The Role of Energy in Türkiye-Russia Relations: A View from the Perspective of Mutual Dependence
Authors: Suyundikov Suinbay , Gökbel Ahmet
Published: December 2023

Policies focusing on strategic energy resources play a key role in achieving certain goals of states by integrating them with other areas. Energy, in particular, has strategic importance and is one of the most critical issues in this context. Considering the opportunities and threats associated with strategic energy resources, the energy problem may be perceived as a chronic ailment that is difficult to solve for some states. It is also a fact that these resources increase the dependence of states. Energy is seen as a factor that forms the basis of interdependence in Turkish-Russian relations. With the increase in Türkiye’s energy needs, its energy relations with Russia have gained great importance in the 21st century. Russia, with its rich energy resources, plays an important role in Türkiye’s energy supply, and Türkiye is an important market for Russia’s energy exports. This study analyses the energy relations between Türkiye and Russia within the framework of interdependence theory. Despite the tensions in the political arena, energy relations have been and continue to be conducted according to the principle of interdependence. The study first introduces the interdependence theory and then analyses the energy policies and energy situation of both countries in detail. At the end of the study, the course of energy relations between Türkiye and Russia is analyzed, and a detailed evaluation is made.
Kazakhstan’s Strategic Priorities in the Turkic World
Authors: Tukumov Yerkin
Published: December 2023

With its unwavering focus on institutionalizing cooperation within the Turkic World, Kazakhstan has successfully achieved this critical objective. Looking ahead, Kazakhstan recognizes the strategic significance of its engagement with the Organization of Turkic States (OTS) member states, navigating the intricate balance of geopolitical challenges and opportunities. This commentary aims to provide an in-depth analysis of the evolving trends in cooperation and outline Kazakhstan’s strategic priorities in the short and medium term. The author suggests that Turkic nations are likely to collaborate in trade, transport, and high-tech ventures and tackle common issues, notably water resource management. This evidences the pivotal role of OTS in creating the essential geopolitical balance within Kazakhstan’s intricate web of institutional connections.
Shaping Eurasia's Future: The Evolving Role and Impact of the Organization of Turkic States
Authors: Kocaman Ömer
Published: December 2023

This commentary provides a comprehensive analysis of the Organization of Turkic States (OTS), tracing its evolution from the dissolution of the Soviet Union to its recent summits. Specifically, it discusses the establishment of the OTS, its institutionalization, and its role in regional and international politics. Furthermore, this analysis delves into the organization's evolution, project execution, and growing global impact, with a particular emphasis on the OTS' commitment to cooperation and solidarity among Turkic states.
A standardized hands-on protocol effectively enhanced orthodontic education on bonding clear aligner attachments
Authors: Li Shijia , Mei Li , Yi Jianru , Abdulghani Ehab A. , Zheng Wei , Li Yu
Published: December 2023

Objectives:

The objectives of this study were to develop and evaluate the effectiveness of a hands-on approach for training orthodontic postgraduates to bond clear aligner (CA) attachments.

Material and Methods:

Twenty orthodontic novice postgraduate students were recruited for the study and randomly divided into two groups. The training group (n = 10) received theory teaching followed by a hands-on training approach; the control group (n = 10) received only theory teaching. The students’ performance of bonding attachments, including bonding failure, marginal flash, and morphological defects, was assessed by a panel of clinical supervisors.

Results:

The hands-on training approach promoted the students’ performance of bonding CA attachments, with an increased success rate (from 34% ± 6% to 81% ± 6%, P < 0.01) and a reduced operating time (from 89 ± 5 min to 65 ± 4 min, P < 0.01) compared with the control group. The most challenging parts of attachment bonding were found to be resin loading, followed by template removal and flash removal.

Conclusion:

The hands-on approach developed in the present study was effective to train orthodontic postgraduates to bond CA attachments, with an increased success rate and reduced operating time.
APPLICATION OF SYNTHETIC WAVEFORMS FOR CREATING A DEEP LEARNING MODEL FOR IDENTIFYING FIRST ARRIVALS IN SEISMIC RECORDS
Authors: Барышников Н.А. , Абзалилов И.А. , Турунтаев С.Б.
Published: December 2023

Задача данной работы – построение метода анализа записей сигналов группы станций, полученных в ходе микросейсмического мониторинга с использованием нейронной сети с целью выделения моментов первых вступлений. Новизна предлагаемого метода заключается в использовании Wavelet Scattering для выделения признаков, что существенно упрощает процесс обучения модели по сравнению с традиционно применяемыми свёрточными нейронными сетями. Использованная в модели архитектура Transformer позволила реализовать обмен информацией между произвольным числом принимающих станций и, тем самым, определять времена вступлений на отдельных станциях на основе информации всей группы. Обучение модели проводилось на полностью синтетических данных. Такой подход по сравнению с обучением на реальных данных может позволить получить модель с лучшей обобщающей способностью, так как синтетические данные отражают механику процесса возникновения и распространения упругих колебаний и при этом не искажены локальными условиями и особенностями регистрации сигналов. Исследован ряд стратегий аугментации (модификации данных обучающей выборки) путём наложения шума с целью увеличения устойчивости модели к шуму. Наилучшее соотношение устойчивости к шуму и точности модели было получено при наложении Гауссовского шума со случайным стандартным отклонением. Результаты работы могут быть использованы при построении моделей глубокого обучения, обучаемых как на синтетических, так и на реальных сейсмических записях.

The goal of this study was to develop a method for analyzing signal records from a group of stations acquired during microseismic monitoring using a neural network to identify the moments of first arrivals. The novelty of the proposed method lies in the use of Wavelet Scattering for feature extraction, which significantly simplifies the model training process compared to traditionally used convolutional neural networks. The Transformer architecture used in the model allows for information exchange between an arbitrary number of recording stations, thereby determining arrival times at individual stations based on information from the entire group. The model was trained on fully synthetic data. This approach, compared to training on real data, may lead to a model with better generalization capabilities, as synthetic data reflect the mechanics of the generation and propagation of elastic waves without being influenced by local conditions and signal registration features. Several data augmentation strategies were explored, including adding noise to increase the model’s robustness to noise. The best balance between noise robustness and model accuracy was achieved by adding Gaussian noise with random standard deviation. The results of this study can be used in the development of deep learning models trained on both synthetic and real seismic records.
Formación en turismo: Un enfoque ético, sostenible y consciente
Authors: Rodrigues Soares Jakson Renner , Barreto Lopes Aurelinda , Solla Santos Xosé
Published: December 2023

El modelo tradicional del turismo fue pensado durante muchos años desde una dimensión económica. Sin embargo, recientemente y debido a los impactos generados por la actividad turística, nuevas modalidades de turismo han sido pensadas, poniendo el foco de la experiencia no solo en lo económico, también en otros aspectos capaces de generar desarrollo. Este artículo, es el resultado de una investigación sobre la importancia de la formación de los profesionales del turismo, para garantizar la realización de actividades equilibradas, que respeten y preserven el medio ambiente en los destinos turísticos. Desde la perspectiva profesional, las personas que actúan en el turismo también son responsables por la continuidad de esta actividad. El objetivo fue comprender cómo afecta el proceso de formación de los profesionales del turismo, al desarrollo de un turismo consciente; de esta manera, crea relación entre los conceptos que le conforman. El estudio cualitativo, permitió traducir los conceptos y esbozar la participación de los profesionales del turismo, en la preservación del medio ambiente y de la actividad misma.
Can R2BX4/ABX3 Heterostructures Harmonise Stability and Efficiency in Perovskite Photovoltaics? A Minireview
Authors: Abbas Muhammad Sohail , Ahmad Rashid , Khan Mashooq
Published: December 2023

Halide perovskites are one of the most extensively explored materials for photovoltaics and their photo-conversion efficiency has reached over 25%. Three-dimensional (3D) perovskites have exceptional optoelectronic properties but suffer from poor stability, hindering their commercialization. Contrarily, two-dimensional (2D) perovskites offer excellent stability but inferior optoelectronic performance. The structural flexibility of perovskites allows the researcher to fabricate 2D/3D mixed dimensional perovskites which exhibit superior stability without compromising the efficiency. Herein, we have explored the available literature on a distinct category of 2D/3D perovskites, R2BX4/ABX3 type mixed phase heterostructures. In such heterostructures, a minor quantity of phase pure 2D perovskite coincides with a bulk of 3D perovskites. The compositional development, structural variations, and controlling the level of 2D doping in this kind of perovskites have been discussed. Moreover, film quality, photophysics, photovoltaic performance and stability of such heterostructures were also discussed. Finally, our efforts aimed to offer a glimpse into the potential future applications of R2BX4/ABX3 in improving perovskite photovoltaics and other applications beyond solar cells.
Siyasi Süreç Modeli ve Tunus’ta “İslami Eğilim Hareketi”
Authors: SİVRİKAYA Halil Atilla
Published: December 2023

En-Nahda Hareketi’nin öncülü olan İslami Eğilim Hareketi’ne (İEH) odaklanan bu makalenin temel sorusu, 1981-1989 arası dönemde İEH’nin Tunus’taki toplumsal hareketlilik sürecini açıklamaktır. Bu makale, İEH’nin Tunus’ta neden ve nasıl ortaya çıktığını, bir siyasi parti kurduğunu, Tunus’taki diğer hareketlerden farklı hedefler yarattığını ve ittifak tercihlerinde, stratejilerinde ve politikalarında dikkate değer bir ılımlılık, pragmatizm ve esneklik gösterdiğini incelemektedir. Bu makale, İEH’yi Sosyal Hareket Teorisi (SHT) doğrultusunda incelemekte ve bu bağlamda, Sidney George Tarrow, Doug McAdam ve Charles Tilly tarafından geliştirilen Siyasi Süreç Modeli’ni (SSM) uygulamaktadır. Makale, SSM’yi uygulayarak Batılı olmayan yarı otoriter bir siyasi ortamda toplumsal hareketliliği incelemenin yanı sıra söz konusu dönemde değişen ve dönüşen siyasi fırsat yapılarını, örgütsel dinamikleri ve çerçeveleme süreçlerini ortaya çıkararak literatüre katkıda bulunmaktadır. İEH örneğinden yola çıkan bu makale, İslami bir hareketin devlet baskısına rağmen siyasi fırsat yapıları, örgütsel dinamikler ve çerçeveleme süreçleri aracılığıyla ılımlılıkla ayakta kalabileceğini ve tabanını harekete geçirebileceğini savunmaktadır.
21. Yüzyılda Türkiye’nin Dış Politikası
Authors: BALTA Pınar Hilal
Published: December 2023

Prof. Dr. Ramazan ERDAĞ imzasıyla yayınlanan "21. YÜZYILDA TÜRKİYE’NİN DIŞ POLİTİKASI" başlıklı kitabın tanıtım yazısı.