bluuebunny/crossref_metadata_2025 huggingface dataset:
Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia
Authors: Yang Yanzhuo , Yu Qingting , Li Bin , Yang Zuisu , Zhang Shuai , Yuan Falei
Published: April 2023

Palmitic acid (PA) is considered a major contributor to the inflammation in many metabolic diseases; however, this role has been questioned recently for the complicated procedures in preparing PA-bovine serum albumin (BSA) complex. This study is aimed to evaluate the effect of PA-BSA complexing methods on cell viability and inflammatory responses of BV-2 cells. Three commercially available BSA brands and two types of solvents were compared for their effects on the expression of inflammatory cytokines. Three commonly used proportions of PA-BSA were tested for cell viability and inflammatory responses. We found that all the three types of BSA were proinflammatory. Both ethanol and isopropanol dampened inflammation except that 1% isopropanol treatment increased the IL-1β level by 26%. When reducing the BSA content in PA-BSA solutions from 3:1 to 5:1, a marked increase in cell viability (11%) was seen. To our surprise, reducing BSA content in PA-BSA solutions from 5:1 to 10:1 decreased cell viability by 11%. The 5:1 group exhibited the lowest inflammatory profile. Either PA-BSA or BSA alone increased the entry of LPS to the cytosol, which further caused pyroptosis. In summary, we found 5:1 (PA:BSA) to be the best binding ratio for studying inflammation in BV-2 microglia. The presence of LPS in the cytosol in the context of BSA might be the reason for confounding results from palmitate studies.
Naupliar exposure to acute warming does not affect ontogenetic patterns in respiration, body size, or development time in the cosmopolitan copepod Acartia tonsa
Authors: Holmes-Hackerd Mathew , Sasaki Matthew , Dam Hans G.
Published: April 2023

Short-term, acute warming events are increasing in frequency across the world’s oceans. For short-lived species like most copepods, these extreme events can occur over both within- and between-generational time scales. Yet, it is unclear whether exposure to acute warming during early life stages of copepods can cause lingering effects on metabolism through development, even after the event has ended. These lingering effects would reduce the amount of energy devoted to growth and affect copepod population dynamics. We exposed nauplii of an ecologically important coastal species,Acartia tonsa, to a 24-hour warming event (control: 18°C; treatment: 28°C), and then tracked individual respiration rate, body length, and stage duration through development. As expected, we observed a decrease in mass-specific respiration rates as individuals developed. However, exposure to acute warming had no effect on the ontogenetic patterns in per-capita or mass-specific respiration rates, body length, or development time. The lack of these carryover effects through ontogeny suggests within-generational resilience to acute warming in this copepod species.
Efficacy and safety of curcumin in diabetic retinopathy: A protocol for systematic review and meta-analysis
Authors: Wang Liyuan , Xu Jiayu , Yu Tianyang , Wang Hanli , Cai Xiaojun , Sun He
Published: April 2023

Background

Diabetic retinopathy (DR) is one of the most common complications of diabetes and has become a major global cause of blindness. Curcumin, an extract of Curcuma longa (turmeric), is effective in preventing and treating diabetes. Recent studies have shown that curcumin can delay DR development. However, there has been no systematic review of its treatment of DR. This study will conduct a systematic review and meta-analysis of currently published randomized controlled trials (RCT) of curcumin for treating DR patients to evaluate its efficacy and safety.

Methods

We will search the relevant studies of curcumin in the treatment of DR in PubMed, Medline, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), VIP, and Wanfang databases from their respective inception dates to May 2022. A meta-analysis of the data extracted from qualified RCTs will be conducted, including the progression of DR, visual acuity, visual field, macular edema, quality of life, and adverse events. The meta-analysis will be performed using Review Manager 5.4.1 software, and the results will be based on either random-effects or fixed-effects models, depending on the heterogeneity. The Grading of Recommendations, Development, and Evaluation (GRADE) system will be used to evaluate the reliability and quality of evidence.

Results

The results of this study will provide sound and high-quality evidence for the efficacy and safety of curcumin in the treatment of DR.

Conclusion

This study will be the first meta-analysis to comprehensively assess the efficacy and safety of curcumin in the treatment of DR and will provide helpful evidence for the clinical management of this disease.

Systematic review registration

INPLASY202250002.
Epicardial electrical heterogeneity after amiodarone treatment increases vulnerability to ventricular arrhythmias under therapeutic hypothermia
Authors: Lin Chin-Yu , Chang Ting-Yung , Hu Yu-Feng , Hsieh Yu-Cheng , Chen Yi-Jen , Yeh Hung-I , Lin Yenn-Jiang , Chang Shih-Lin , Lo Li-Wei , Chao Tze-Fan , Chung Fa-Po , Liao Jo-Nan , Tuan Ta-Chuan , Chen Shih-Ann
Published: April 2023

Background

Amiodarone is commonly used during therapeutic hypothermia (TH) following cardiac arrest due to ventricular arrhythmias. However, electrophysiological changes and proarrhythmic risk after amiodarone treatment have not yet been explored in TH.

Methods

Epicardial high-density bi-ventricular mapping was performed in pigs under baseline temperature (BT), TH (32–34°C), and amiodarone treatment during TH. The total activation time (TAT), conduction velocity (CV), local electrogram (LE) duration, and wavefront propagation from pre-specified segments were analyzed during sinus rhythm (SR) or right ventricular (RV) pacing (RVP), along with tissue expression of connexin 43. The vulnerability to ventricular arrhythmias was assessed.

Results

Compared to BT, TH increased the global TAT, decreased the CV, and generated heterogeneous electrical substrate during SR and RVP. During TH, the CV reduction and LE duration prolongation were greater in the anterior mid RV than in the other areas, which changed the wavefront propagation in all animals. Compared to TH alone, amiodarone treatment during TH further increased the TAT and LE duration and decreased the CV. Heterogeneous conduction was partially attenuated after amiodarone treatment. After TH and amiodarone treatment, the connexin 43 expression in the anterior mid RV was lower than that in the other areas, compatible with the heterogeneous CV reduction. The animals under TH and amiodarone treatment had a higher incidence of inducible ventricular arrhythmias than those under BT or TH without amiodarone.

Conclusion

Electrical heterogeneity during amiodarone treatment and TH was associated with vulnerability to ventricular arrhythmias.
A group decision making method with preference analysis to re-build the Global Entrepreneurship Index
Authors: Chen Caixiang , Mo Shiliang , Zhao Jinling
Published: April 2023

This study proposes a group decision making (GDM) method with preference analysis to re-build the Global Entrepreneurship Index (GEI). Specifically, a single decision maker is firstly identified using a specified individual judgement about the importance order of three sub-indices of the GEI. A preliminary group decision matrix is constructed in terms of taking all possible individual judgments into account. Then the analysis of the preferential differences and preferential priorities with respect to the preliminary group decision matrix is conducted to obtain a revised group decision matrix, in which preferential differences calculate the weighted differences as the degrees of differences among different alternatives for each decision maker, preferential priorities describe the favorite ranking of alternatives for each decision maker. Finally, we employ the Stochastic Multicriteria Acceptability Analysis for group decision-making (SMAA-2) to create the holistic acceptability indices for measuring the entrepreneurship performance. In addition, a satisfaction index is developed to indicate the merits of proposed GDM method. A case study using the GEI-2019 of 19 G20 countries is carried out to validate our GDM method.
Authorization of midwives to perform basic emergency obstetric and newborn care signal functions in Argentina, Ghana, and India: A multi-country validation study of a key global maternal and newborn health indicator
Authors: Ramesh Sowmya , Chakraborty Suchandrima , Adanu Richard M. , Bandoh Delia A. B. , Berrueta Mabel , Gausman Jewel , Khan Nizamuddin , Kenu Ernest , Langer Ana , Nigri Carolina , Odikro Magdalene A. , Pingray Verónica , Saggurti Niranjan , Vázquez Paula , Williams Caitlin R. , Jolivet R. Rima
Published: April 2023

Background

Midwives’ authorization to deliver the seven basic emergency obstetric and newborn care (BEmONC) functions is a core policy indicator in global monitoring frameworks, yet little evidence supports whether such data are captured accurately, or whether authorization demonstrates convergence with midwives’ skills and actual provision of services. In this study, we aimed to validate the data reported in global monitoring frameworks (criterion validity) and to determine whether a measure of authorization is a valid indicator for BEmONC availability (construct validity).

Methods

We conducted a validation study in Argentina, Ghana, and India. To assess accuracy of the reported data on midwives’ authorization to provide BEmONC services, we reviewed national regulatory documents and compared with reported country-specific data in Countdown to 2030 and the World Health Organization Maternal, Newborn, Child and Adolescent Health Policy Survey. To assess whether authorization demonstrates convergent validity with midwives’ skills, training, and performance of BEmONC signal functions, we surveyed 1257 midwives/midwifery professionals and assessed variance.

Results

We detected discrepancies between data reported in the global monitoring frameworks and the national regulatory framework in all three countries. We found wide variations between midwives’ authorization to perform signal functions and their self-reported skills and actual performance within the past 90 days. The percentage of midwives who reported performing all signal functions for which they were authorized per country-specific regulations was 17% in Argentina, 23% in Ghana, and 31% in India. Additionally, midwives in all three countries reported performing some signal functions that the national regulations did not authorize.

Conclusion

Our findings suggest limitations in criterion and construct validity for this indicator in Argentina, Ghana, and India. Some signal functions such as assisted vaginal delivery may be obsolete based on current practice patterns. Findings suggest the need to re-examine the emergency interventions that should be included as BEmONC signal functions.
Major dietary patterns of community dwelling adults and their associations with impaired blood glucose and central obesity in Eastern Ethiopia: Diet-disease epidemiological study
Authors: Mekonnen Berhanu Abebaw , Oumer Abdu , Ale Ahmed , Hamza Aragaw , Dagne Imam , Umer Abdurezak Adem , Fentie Dilnessa , Yigezu Muluken , Tariku Zerihun , Abate Shambel
Published: April 2023

Backgrounds

Unhealthy dietary intake is an important preventable risk factor for obesity and impaired blood glucose (IBG), ultimately increasing the risk of non-communicable diseases. When compared to individual food intakes, dietary patterns are a stronger predictor of health outcomes and should be systematically evaluated where such evidence is lacking. This study evaluated dietary patterns and their association with the risk of central obesity and IBG among adults.

Methods

A community-based survey was conducted among 501 randomly-selected adults from Eastern Ethiopia. Data was collected using a semi-structured questionnaire during a face-to-face interview that included sociodemographic and lifestyle factors, as well as a validated 89-item food frequency questionnaire (collected over one month). Principal component analysis was used to derive the dietary pattern. While central obesity was assessed using waist and/or hip circumference measurements, fasting blood sugar was used for IBG. A multivariable logistic regression model was fitted with an odds ratio, 95% confidence intervals, and p-values reported.

Results

A total of 501 adults (95.3%) were interviewed, with a mean age of 41 years (±12). Five major dietary patterns explaining 71% of the total variance were identified: “nutrient-dense foods”, “high fat and protein”, “processed foods”, “alcohol drinks”, and “cereal diets”. While 20.4% (17.0–24.2%) had IBG, 14.6% (11.8–17.9) were centrally obese, and 94.6% (92.3–96.3) had an increased waist-to-hip circumference ratio. Central obesity is associated with upper wealth status (AOR = 6.92; 2.91–16.5), physical inactivity (AOR = 21.1; 2.77–161.4), a diet high in nutrient-dense foods (AOR = 1.75; 0.75–4.06), processed foods (AOR = 1.41; 0.57–3.48), and cereal diets (AOR = 4.06; 1.87–8.82). The burden of IBG was associated with upper wealth status (AOR = 2.36; 1.36–4.10), physical inactivity (AOR = 2.17; 0.91–5.18), upper tercile of nutrient-dense foods (AOR = 1.35; 0.62–2.93), fat and protein diet (AOR = 1.31; 0.66–2.62), and cereal diet consumption (AOR = 3.87; 1.66–9.02).

Conclusion

IBG and central obesity were prevalent and predicted by upper tercile consumption of nutrient-dense foods, high fat and protein diets, processed foods, and cereal diets, which could guide dietary interventions.
Stress and benefits of video calling for people with autism spectrum disorders
Authors: Yuruki Kengo , Inoue Masahiko
Published: April 2023

This study compared stress and the benefits of video calling between individuals with autism spectrum disorder (ASD) proneness and diagnosis and those with typical developmental (TD). Study participants were recruited via the web, and 151 of the 252 participants who responded to a web-based questionnaire were included in the analysis (ASD group:76; TD group:75). The results of the chi-square test suggest that the ASD group may prefer video calling more than the TD group. The results of the analysis using a qualitative methodology (KJ method) suggested that the ASD group was more likely than the TD group to perceive stress due to light stimuli emitted from the screen and the inability to concentrate on a conversation due to visual stimuli. The ASD group perceived the ability to cope with stressful stimuli by operating the device as an benefits of video calling. These findings suggest the importance of creating a communication environment that reduces stress and maximizes the benefits of video calling for people with ASD. Specific support measures include establishing rules in advance that allow the individual to turn the video off or switch to texting.
Omega-3 supplements in the prevention and treatment of youth depression and anxiety symptoms: A scoping review
Authors: Reily Natalie M. , Tang Samantha , Negrone Ashlee , Gan Daniel Z. Q. , Sheanoda Veronica , Christensen Helen
Published: April 2023

Introduction

Omega-3 supplements may be efficacious in reducing symptoms of depression and anxiety in adults, particularly as an adjunct to antidepressant medication. However, research in young people is limited. Thus, this scoping review aimed to summarise existing evidence on the efficacy of omega-3 supplementation in treating depression and anxiety symptoms in young people aged 14–24. A secondary aim was to determine whether grey literature intended for the general public accurately reflects the evidence.

Method

Four databases (Cochrane CENTRAL, EmBASE, PsycINFO, PubMed) were searched from inception to 4th August 2021. Eligible peer-reviewed studies were empirical studies which examined the efficacy of omega-3 supplements in preventing/treating anxiety and/or depression symptoms in young people aged 14–24. Risk of bias was assessed for randomised studies using the Cochrane Risk of Bias Tool. Selected grey literature databases were also searched, with eligible sources assessed for quality. A stakeholder group including young people with lived experience of anxiety/depression, parents/carers and mental health professionals informed the research questions and data interpretation. Findings were summarised using narrative synthesis.

Results

17 empirical studies (N = 1240 participants) meeting inclusion criteria were identified. Studies varied in treatment and participant characteristics. In general, the data did not support the view that omega-3 supplements were efficacious in improving symptoms of anxiety or depression in young people aged 14–24. In contrast, most grey literature sources recommended the use of omega-3 supplements in young people.

Discussion

Evidence for efficacy of omega-3 supplementation in reducing symptoms of depression and anxiety in young people was inconclusive. More research is needed to identify potential mechanisms and moderators of the effect of omega-3 supplements on depression and anxiety symptoms in young people.
A scoping review of medication self-management intervention tools to support persons with traumatic spinal cord injury
Authors: Cadel Lauren , Cimino Stephanie R. , Bradley-Ridout Glyneva , Hitzig Sander L. , Packer Tanya L. , McCarthy Lisa M. , Patel Tejal , Lofters Aisha K. , Hahn-Goldberg Shoshana , Ho Chester H. , Guilcher Sara J. T.
Published: April 2023

Background

Persons with traumatic spinal cord injury (SCI) use multiple medications (polypharmacy) to manage the high number of secondary complications and concurrent conditions. Despite the prevalence of polypharmacy and challenges associated with managing medications, there are few tools to support medication self-management for persons with SCI.

Objective

The purpose of this scoping review was to identify and summarize what is reported in the literature on medication self-management interventions for adults with traumatic SCI.

Methods

Electronic databases and grey literature were searched for articles that included an adult population with a traumatic SCI and an intervention targeting medication management. The intervention was required to incorporate a component of self-management. Articles were double screened and data were extracted and synthesized using descriptive approaches.

Results

Three studies were included in this review, all of which were quantitative. A mobile app and two education-based interventions to address self-management of SCI, medication management, and pain management, respectively, were included. Only one of the interventions was co-developed with patients, caregivers, and clinicians. There was minimal overlap in the outcomes measured across the studies, but learning outcomes (e.g., perceived knowledge and confidence), behavioural outcomes (e.g., management strategies, data entry), and clinical outcomes (e.g., number of medications, pain scores, functional outcomes) were evaluated. Results of the interventions varied, but some positive outcomes were noted.

Conclusions

There is an opportunity to better support medication self-management for persons with SCI by co-designing an intervention with end-users that comprehensively addresses self-management. This will aid in understanding why interventions work, for whom, in what setting, and under what circumstances.
Do network synergies facilitates the realization of M&A motivation?: From the perspective of network node degree and strength change
Authors: Zhang Ziqiao , Zhang Qiusheng , Li Chaofeng
Published: April 2023

The current evaluation of M&A performance lacks consideration of M&A motives. In this paper, we theoretically analyse and empirically test the effect of network synergy generated by M&A on the degree of realization of corporate M&A motives and the mechanism of its effect by constructing an equity network between a listed company and its subsidiaries within the company. The results show that the greater the variation of internal network node degree and strength, the more beneficial it is to promote the degree of realization of corporate M&A motivation; the results of further mechanism of action tests show that the variation of network node degree and strength has significant effects on economies of scale, economies of scope, and transaction costs; Furthermore, the heterogeneity test finds that the effect of variation of network node degree and strength to promote the degree of realization of corporate M&A motivation is more significant in the case of non-cash payment method and related M&A. This paper extends the study of complex networks to the field of M&A and uniquely explains the paradox of the "high failure rate" of M&A and the increasing activity of M&A activities from the perspective of network synergy, which helps to rationalize the M&A behavior of enterprises and further regulate the M&A behavior of listed companies by regulatory authorities.
In silico EsxG EsxH rational epitope selection: Candidate epitopes for vaccine design against pulmonary tuberculosis
Authors: Martinez-Olivares Constanza Estefania , Hernández-Pando Rogelio , Mixcoha Edgar
Published: April 2023

Rational design of new vaccines against pulmonary tuberculosis is imperative. Early secreted antigens (Esx) G and H are involved in metal uptake, drug resistance, and immune response evasion. These characteristics make it an ideal target for rational vaccine development. The aim of this study is to show the rational design of epitope-based peptide vaccines by using bioinformatics and structural vaccinology tools. A total of 4.15 μs of Molecular Dynamics simulations were carried out to describe the behavior in solution of heterodimer, single epitopes, and epitopes loaded into MHC-II complexes. In order to predict T and B cell epitopes for antigenic activation, bioinformatic tools were used. Hence, we propose three epitopes with the potential to design pulmonary tuberculosis vaccines. The possible use of the proposed epitopes includes subunit vaccines, as a booster in BCG vaccination to improve its immune response, as well as the generation of antibodies that interfere with the Mycobacterium tuberculosis homeostasis, affecting its survival.
Home-based exercise improves quality of life in breast and prostate cancer survivors: A meta-analysis
Authors: Bates-Fraser Lauren C. , Riley Sasha , Stopforth Cameron , Moertl Kaileigh , Edgar Kyle , Stoner Lee , Hanson Erik D.
Published: April 2023

Background

Breast (BCa) and prostate (PCa) cancer are two of the most common but survivable cancers. One important component of survivorship that is impacted by treatment long term is diminished quality of life (QoL). Supervised exercise improves QoL and subsequent outcomes but is not accessible for all survivors. Additionally, many factors influence QoL including physical activity (PA), cardiorespiratory fitness (CRF), physical function, and fatigue. However, the COVID-19 pandemic has highlighted the need to increase access to exercise beyond supervised exercise facilities. Home-based exercise may provide a feasible alternative for cancer survivors especially for those living in rural communities.

Objectives

The primary aim is to investigate the effects of home-based exercise training (Pre-training vs. Post-training) on QoL in BCa/PCa. A secondary aim is to investigate PA, CRF, physical function, and fatigue and potential moderators (age, cancer-type, intervention duration and type). Home-based exercise trials (randomized crossover or quasi-experimental design) with adults (aged 18 years and over) breast or prostate cancer survivors (not currently undergoing chemotherapy or radiation treatment) were eligible for inclusion.

Data sources

Electronic databases were searched (inception-December 2022) for studies which included adult BCa or PCa survivors (not currently on chemotherapy/radiation), at least measured QoL, and undergoing unsupervised, home-based exercise training.

Appraisal and synthesis methods

Initially, 819 studies were identified, from which 17 studies (20 effects) involving 692 participants were extracted. Effect sizes were calculated as standardized mean differences (SMD). Data were pooled using a 3-level model with restricted maximum likelihood estimation. Pooled SMD was used to assess the magnitude of effect, where <0.2, 0.2, 0.5, and 0.8 was defined as trivial, small, moderate, and large respectively.

Results

Home-based exercise resulted in small improvements in QoL (SMD = 0.30, 95% CI 0.01, 0.60, p = 0.042), PA (SMD = 0.49, 95% CI 0.26, 0.75, p<0.001) and CRF (SMD = 0.45, 95% CI -0.01, 0.91, p = 0.056). Physical function (SMD = 0.00, 95% CI -0.21, 0.21, p = 1.000) and fatigue (SMD = -0.61, 95%CI -1.53, 0.32, p = 0.198) did not change.

Conclusions

Home-based exercise results in small improves QoL in BCa/PCa survivors, independent of cancer type, intervention duration and type, or age. Home-based exercise also improves PA and CRF enhancing survivorship. Therefore, home-based exercise is an efficacious alternative option to improve QoL for BCa and PCa survivors especially for those who live in rural communities or lack access to exercise facilities.
Longitudinal models for the progression of disease portfolios in a nationwide chronic heart disease population
Authors: Holm Nikolaj Normann , Frølich Anne , Andersen Ove , Juul-Larsen Helle Gybel , Stockmarr Anders
Published: April 2023

Background and aim

With multimorbidity becoming increasingly prevalent in the ageing population, addressing the epidemiology and development of multimorbidity at a population level is needed. Individuals subject to chronic heart disease are widely multimorbid, and population-wide longitudinal studies on their chronic disease trajectories are few.

Methods

Disease trajectory networks of expected disease portfolio development and chronic condition prevalences were used to map sex and socioeconomic multimorbidity patterns among chronic heart disease patients. Our data source was all Danish individuals aged 18 years and older at some point in 1995-2015, consisting of 6,048,700 individuals. We used algorithmic diagnoses to obtain chronic disease diagnoses and included individuals who received a heart disease diagnosis. We utilized a general Markov framework considering combinations of chronic diagnoses as multimorbidity states. We analyzed the time until a possible new diagnosis, termed the diagnosis postponement time, in addition to transitions to new diagnoses. We modelled the postponement times by exponential models and transition probabilities by logistic regression models.

Findings

Among the cohort of 766,596 chronic heart disease diagnosed individuals, the prevalence of multimorbidity was 84.36% and 88.47% for males and females, respectively. We found sex-related differences within the chronic heart disease trajectories. Female trajectories were dominated by osteoporosis and male trajectories by cancer. We found sex important in developing most conditions, especially osteoporosis, chronic obstructive pulmonary disease and diabetes. A socioeconomic gradient was observed where diagnosis postponement time increases with educational attainment. Contrasts in disease portfolio development based on educational attainment were found for both sexes, with chronic obstructive pulmonary disease and diabetes more prevalent at lower education levels, compared to higher.

Conclusions

Disease trajectories of chronic heart disease diagnosed individuals are heavily complicated by multimorbidity. Therefore, it is essential to consider and study chronic heart disease, taking into account the individuals’ entire disease portfolio.
Characterization of multivalent complexes formed in the presence of more than one conventional antibody to terminal complement component C5
Authors: Cone Josh , Kimmel Lida , Zhang Yuchun , Johnson Krista , Sheridan Douglas , Tamburini Paul
Published: April 2023

This study sought to understand the nature of the immune complexes that could be formed when a patient is exposed simultaneously to two different anti-complement component 5 (C5) antibodies, such as in patients converting from one bivalent, noncompetitive, C5-binding monoclonal antibody to another. Size exclusion chromatography (SEC) in combination with multiangle light scattering was used to assess the potential formation of multivalent complexes among eculizumab, C5, and each of two other anti-C5 bivalent antibodies, TPP-2799 or TP-3544, respectively having the same sequence as either crovalimab or pozelimab currently undergoing clinical trials. Each of these two antibodies bound C5 noncompetitively with eculizumab. In phosphate-buffered saline (PBS), C5-eculizumab in the absence of other antibodies measured <500 kDa; however, inclusion of other antibodies at levels ranging from equimolar and up to a fivefold excess over eculizumab and C5 yielded a series of complexes with some >1500 kDa in size, consistent with incorporation of multiple antibodies and C5 molecules. A similar pattern of complexes was also observed when fluorescently labeled eculizumab and either of the other two antibodies were spiked into human plasma, based on SEC monitored by fluorescence detection. A detailed characterization of the pharmacodynamic and pharmacokinetic properties of such complexes is warranted, as is the incorporation of mitigation processes to avoid their formation in patients converting from one bivalent, noncompetitive, C5-binding monoclonal antibody to another.
Impact of polyacrylamide supplementation on intake, nutrient digestion and growth of lambs
Authors: Zhai Zhixiang , Luo Qiujiang , Chen Yong , Pan Ron , Zang Changjiang
Published: April 2023

This study was conducted to evaluate the effect of polyacrylamide (PAM) supplementation on the intake, digestion, weight gain, metabolism and growth of lambs. A total of ten 30 days old male small-tailed Han lambs with a body weight of 7.7±0.5 kg were divided into two equal groups (n = 5 each) and fed a basal diet or diet supplemented with 2.0 g of PAM per kg diet. The duration of the experiment was 210 days and experimental diets were fed ad libitum throughout the experimental period. Voluntary feed intake (VFI) was measured on daily basis, while body weight was measured on every ten days of the experiment.Two digestive and metabolic trials were conducted at the lamb’s age of 95 to 103 days (Trial 1) and at the age of 210 to 218 days (Trial 2). At the end of experiment, all lambs were slaughtered to determine carcass characteristics. Results of the current study showed that supplementation of PAM in the diet of lambs increased the VFI and daily body gain by 14.4% (P < 0.05) and 15.2% (P < 0.01), respectively. In Trial 1, PAM supplementation in the diet increased the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), cellulose, energy, and nitrogen retention by 7.9%, 5.4%, 6.4%, 9.6%, 4.3% and 30.3% (P < 0.01), respectively, and in Trial 2, PAM supplementation in the diet increased the digestibility of DM, OM, CP, cellulose, energy, and nitrogen retention by 9.3%, 7.9%, 7.7%, 11.6%, 6.9% and 38.5% (P < 0.01), respectively. Results of carcass parameter explored that supplementation of PAM in the diet increased the carcass, net meat and lean meat weights by 24.5%, 25.5%, and 30.6% (P < 0.01), respectively, however, PAM supplementation in the diet did not influence the contents of DM, OM, or CP in fresh liver, leg muscle, and rumen tissue; in addition, the CP contents in the Longissimus dorsi muscle was decreased by the supplementation of PAM in the diet. In summary, supplementation of 2.0 g of PAM per kg diet increased the VFI, nutrient digestibility, nitrogen retention, and carcass yield of lambs.
Women empowerment through involvement in community-based health and nutrition interventions: Evidence from a qualitative study in India
Authors: Pradhan Manas Ranjan , Unisa Sayeed , Rawat Ramu , Surabhi Somila , Saraswat Abhishek , R. S. Reshmi , Sethi Vani
Published: April 2023

Women’s empowerment is fundamental for realizing unalienable human rights and is vital to sustainable development outcomes. In India, the SWABHIMAAN intervention program was an integrated multi-sectoral strategy to improve girls’ and women’s nutrition before conception, during pregnancy, and after childbirth. This study assesses the role of self-help-group (SHGs) in improving the effectiveness of community health interventions and its impact on their self-empowerment. Qualitative data gathered through in-depth interviews (IDI) with community-based SHG members involved as Nutrition Friend (Poshan Sakhi-PS) in the SWABHIMAAN program in 2018 was used for analysis. Informed consent procedures were followed, and only those who voluntarily consented to the interview were interviewed. Twenty-five IDIs of purposively selected PSs in three states (Bihar, n = 9; Chhattisgarh, n = 8; and Odisha, n = 8) were analyzed thematically, according to Braun & Clarke (2006). NVivo 12 software was used for organizing and coding data. Three central themes that emerged to explain women’s empowerment were (1) Barriers & redressal mechanisms adopted by PS, (2) PS as a change-maker, and (3) Changes in the life of PS. The study found that women perceive themselves as more empowered through involvement in the SWABHIMAN intervention program, besides improving the community’s and their households’ nutritional status. The results suggest that policies and programs on health and nutrition interventions need to involve peer women from the community, leading to more effective outcomes. Empowering women and closing gender gaps in employment/work are critical to achieving the 2030 Sustainable Development Goals.
Hepcidin expression in the trigeminal ganglion and the oral mucosa in an oral ulcerative mucositis rat model
Authors: Hitomi Suzuro , Nodai Tomotaka , Kokabu Shoichiro , Shikayama Takemi , Sago-Ito Misa , Nakatomi Chihiro , Terawaki Kiyoshi , Omiya Yuji , Shinoda Masamichi , Ono Kentaro
Published: April 2023

Severe intraoral pain induces difficulty in eating and speaking, leading to a decline in the quality of life. However, the molecular mechanisms underlying intraoral pain remain unclear. Here, we investigated gene modulation in the trigeminal ganglion and intraoral pain-related behavior in a rat model of acetic acid-induced oral ulcerative mucositis. Oral ulceration was observed on day 2 after acetic acid treatment to the oral mucosa of male Wistar rats, causing spontaneous pain and mechanical allodynia. Deoxyribonucleic acid microarray analysis of trigeminal ganglion tissue indicated that Hamp (a hepcidin gene that regulates cellular iron transport) was the most upregulated gene. In the oral ulcerative mucositis model, the upregulation of Hamp was also induced in the ulcer region but not in the liver, with no increase in hepcidin levels in the plasma and saliva, indicating that hepcidin was produced locally in the ulcer region in the model. Systemic antibiotic pretreatment did not increase the mRNA levels of Hamp in the trigeminal ganglion and ulcer regions. Hepcidin injection into the oral mucosa enhanced neuronal excitability in response to noxious mechanical stimulation of the oral mucosa in trigeminal spinal subnucleus interpolaris/caudalis neurons. These results imply that oral ulcerative mucositis induces oral mucosal pain because of infectious inflammation of the ulcerative area and potentiates Hamp, which represents anti-bacterial and anti-peptidase gene expression in the ulcer region and trigeminal ganglion. The regulation of cellular iron transport by hepcidin is likely involved in oral ulcerative mucositis-induced pain.
Prenatal and early-life exposure to traffic-related air pollution and allergic rhinitis in children: A systematic literature review
Authors: Liu Lifang , Ma Jingxuan , Peng Shanshan , Xie Linshen
Published: April 2023

Background

Traffic-related air pollution (TRAP) is hypothesised to play a role in the development of allergic rhinitis (AR). Prenatal and early-life exposure to traffic-related air pollution is considered critical for later respiratory health. However, we could not find any articles systematically reviewing the risk of prenatal and early-life exposure to traffic-related air pollution for allergic rhinitis in children.

Methods

A systematic literature search of PubMed, Web of Science and Medline was conducted to identify studies focused on the association between prenatal and early-life exposure to TRAP and AR in children. Other inclusion criteria were: 1) original articles; 2) based upon prospective or retrospective studies or case-control studies; and 3) publications were restricted to English. Literature quality assessment was processed using the Newcastle-Ottawa Scale (NOS) evaluation scale. This systematic literature review has been registered on the prospero (crd.york.ac.uk/prospero) with the following registry number: CRD42022361179.

Results

Only eight studies met the inclusion criteria. The exposure assessment indicators included PM2.5, PM2.5 absorbance, PM10, NOx, CO, and black carbon. On the whole, exposure to TRAP during pregnancy and the first year of life were positively associated with the development of AR in children.

Conclusions

This systematic review presents supportive evidence about prenatal and early-life exposure to TRAP and the risk of AR in children.
Wolbachia infection in native populations of Blattella germanica and Periplaneta americana
Authors: Choubdar Nayyereh , Karimian Fateh , Koosha Mona , Nejati Jalil , Shabani Kordshouli Razieh , Azarm Amrollah , Oshaghi Mohammad Ali
Published: April 2023

Cockroaches are significant pests worldwide, being important in medical, veterinary, and public health fields. Control of cockroaches is difficult because they have robust reproductive ability and high adaptability and are resistant to many insecticides.Wolbachiais an endosymbiont bacterium that infects the reproductive organs of approximately 70% of insect species and has become a promising biological agent for controlling insect pests. However, limited data on the presence or strain typing ofWolbachiain cockroaches are available. PCR amplification and sequencing of thewspandgltAgenes were used to study the presence, prevalence and molecular typing ofWolbachiain two main cockroach species,Blattella germanica(German cockroach) andPeriplaneta americana(American cockroach), from different geographical locations of Iran. TheWolbachiaendosymbiont was found only in 20.6% of German cockroaches while it was absent in American cockroach samples. Blast search and phylogenetic analysis revealed that theWolbachiastrain found in the German cockroach belongs toWolbachiasupergroup F. Further studies should investigate the symbiotic role ofWolbachiain cockroaches and determine whether lack ofWolbachiainfection may increase this insect’s ability to tolerate or acquire various pathogens. Results of our study provide a foundation for continued work on interactions between cockroaches, bacterial endosymbionts, and pathogens.
A novel anti-membrane CD30 single-chain variable fragment discovered from the human phage library: A potential targeted immunotherapy
Authors: Chanpong Thanida , Seesuay Watee , Chiangjong Wararat , Jiramornimit Piamsiri , Preedagasamzin Sarinthip , Atjanasuppat Korakot , Jittorntrum Bunyada , Prasongtanakij Somsak , Tawinwung Supannikar , Pukiat Sulada , Saisawang Chonticha , Borwornpinyo Suparerk , Sa-ngiamsuntorn Khanit , Chaichumpa Wanpen , Hongeng Suradej , Anurathapan Usanarat
Published: April 2023

Hodgkin’s lymphoma and anaplastic large cell lymphoma, especially relapsed or refractory diseases, could recently be cured by CD30-targeted immunotherapy. However, the CD30 antigen releases the soluble ectodomain of CD30, which might obscure the targeted therapy. Therefore, the membrane epitope of CD30 (mCD30), left on the cancer cells, might be a prospective target for lymphoma treatment. The discovery of novel mCD30 monoclonal antibodies (mAbs) using phage technology yielded 59 potential human single-chain variable fragments (HuscFvs). Ten candidate HuscFv clones have been selected based on various methods, i.e., direct PCR, ELISA and western blot assays, and nucleotide sequencing techniques. Fortunately, only one potential HuscFv clone, clone #A4, was determined by the prediction of HuscFv-peptide molecular docking and the binding affinity test using isothermal titration calorimetry. Finally, we proved that the HuscFv #A4, which had a binding affinity (Kd) of 421e-9 ± 2.76e-6 M, might be the novel mCD30 mAb. We generated chimeric antigen receptor-modified T lymphocytes using HuscFv #A4 as an antigen detection part (anti-mCD30-H4CART). The cytotoxicity assay of anti-mCD30-H4CART cells showed significant eradication of the CD30-expressing cell line, K562 (p = 0.0378). We found a novel mCD30 HuscFv using human phage technology. We systematically examined and proved that our HuscFv #A4 could specifically eradicate CD30-expressing cancers.
A novel task to investigate vibrotactile detection in mice
Authors: Muller Mariel , Pennartz Cyriel M. A. , Bosman Conrado A. , Olcese Umberto
Published: April 2023

Throughout the last decades, understanding the neural mechanisms of sensory processing has been a key objective for neuroscientists. Many studies focused on uncovering the microcircuit-level architecture of somatosensation using the rodent whisker system as a model. Although these studies have significantly advanced our understanding of tactile processing, the question remains to what extent the whisker system can provide results translatable to the human somatosensory system. To address this, we developed a restrained vibrotactile detection task involving the limb system in mice. A vibrotactile stimulus was delivered to the hindlimb of head-fixed mice, who were trained to perform a Go/No-go detection task. Mice were able to learn this task with satisfactory performance and with reasonably short training times. In addition, the task we developed is versatile, as it can be combined with diverse neuroscience methods. Thus, this study introduces a novel task to study the neuron-level mechanisms of tactile processing in a system other than the more commonly studied whisker system.
The effectiveness of post-processing head and neck CT angiography using contrast enhancement boost technique
Authors: Otgonbaatar Chuluunbaatar , Jeon Pil-Hyun , Ryu Jae-Kyun , Shim Hackjoon , Jeon Sang-Hyun , Ko Sung Min , Kim Hyunjung , Kim Jin Woo
Published: April 2023

Background and purpose

This study aimed to investigate the potential of contrast enhancement (CE)-boost technique in the head and neck computed tomography (CT) angiography in terms of the objective and subjective image quality.

Materials and methods

Consecutive patients who underwent head and neck CT angiography between May 2022 and July 2022 were included. The CE-boost images were generated by combining the subtracted iodinated image and contrast-enhanced image. The objective image analysis was compared for each image with and without CE-boost technique using the CT attenuation, image noise, signal-to-noise-ratio (SNR), contrast-to-noise-ratio (CNR), and image sharpness (full width at half width maximum, FWHM). The subjective image analysis was evaluated by two independent experienced radiologists in the following aspects: the overall image quality, motion artifact, vascular delineation, and vessel sharpness.

Results

A total of 65 patients (mean age, 59.48 ± 13.71 years; range, 24–87 years; 36 women) were included. The CT attenuation of the vertebrobasilar arteries was significantly (p < 0.001) higher in the images obtained using CE-boost technique than in conventional images. Image noise was significantly (p < 0.001) lower for CE-boost images (6.09 ± 1.93) than for conventional images (7.79 ± 1.73). Moreover, CE-boost technique yielded higher SNR (64.43 ± 17.17 vs. 121.37 ± 38.77, p < 0.001) and CNR (56.90 ± 18.79 vs. 116.65 ± 57.44, p < 0.001) than conventional images. CE-boost resulted in shorter FWHM than conventional images (p < 0.001). Higher subjective image quality scores were also demonstrated by the CE-boost than images without CE-boost technique.

Conclusions

In both objective and subjective image analysis, the CE-boost technique provided higher image quality without increasing the flow rate and concentration of contrast media in the head and neck CT angiography. Furthermore, the vessel completeness and delineation were superior in CE-boost images than in conventional images.
Prenatal screening tests and prevalence of fetal aneuploidies in a tertiary hospital in Thailand
Authors: Wongkrajang Preechaya , Jittikoon Jiraphun , Sangroongruangsri Sermsiri , Talungchit Pattarawalai , Ruangvutilert Pornpimol , Panchalee Tachjaree , Chaikledkaew Usa
Published: April 2023

This study evaluated prenatal screening test performance and the prevalence of common aneuploidies at Siriraj Hospital, Thailand. We collected data from screening tests which are first-trimester test, quadruple test, and noninvasive prenatal tests (NIPT) between January 2016 and December 2020. Thirty percent (7,860/25,736) of pregnancies received prenatal screening tests for aneuploidies disorders, and 17.8% underwent prenatal diagnosis tests without screening. The highest percentage of screening tests was first-trimester test (64.5%). The high-risk results were 4% for first-trimester test, 6.6% for quadruple test, and 1.3% for NIPT. The serum screening tests for trisomy 13 and 18 had no true positives; therefore, we could not calculate sensitivity. For the first-trimester test, the sensitivity for trisomy 21 was 71.4% (95% confidence intervals (CI) 30.3–94.9); specificity for trisomy 13 and 18 was 99.9% (95% CI 99.8–99.9); and for trisomy 21 was 96.1% (95% CI 95.6–96.7). For the quadruple test, the specificity for trisomy 18 was 99.6% (95% CI 98.9–99.8), while the sensitivity and specificity for trisomy 21 were 50% (95% CI 26.7–97.3) and 93.9% (95% CI 92.2–95.3), respectively. NIPT had 100% sensitivity and specificity for trisomy 13, 18 and 21, and there were neither false negatives nor false positives. For pregnant women < 35 years, the prevalence of trisomy 13, 18, and 21 per 1,000 births was 0.28 (95% CI 0.12–0.67), 0.28 (95% CI 0.12–0.67), and 0.89 (95% CI 0.54–1.45), respectively. For pregnant women ≥35 years, the prevalence of trisomy 13, 18, and 21 per 1,000 births was 0.26 (95% CI 0.06–1.03), 2.59 (95% CI 1.67–4.01), and 7.25 (95% CI 5.58–9.41), respectively. For all pregnancies, the prevalence of trisomy 13, 18, and 21 per 1,000 births was 0.27 (95% CI 0.13–0.57), 0.97 (95% CI 0.66–1.44), 2.80 (95% CI 2.22–3.52), respectively.