bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia
Authors: Romanenko Maria N. , Shikov Anton E. , Savina Iuliia A. , Shmatov Fedor M. , Nizhnikov Anton A. , Antonets Kirill S.
Published: November 2024

The dispersal of plant pathogens is a threat to the global economy and food industry which necessitates the need to discover efficient biocontrol agents such as bacteria, fungi, etc., inhibiting them. Here, we describe the Bacillus mycoides strain b12.3 isolated from the soil of Olkhon Island in Lake Baikal, Russia. By applying the co-cultivation technique, we found that the strain inhibits the growth of plant pathogens, such as the bacteria Xanthomonas campestris, Clavibacter michiganensis, and Pectobacterium atrospecticum, as well as the fungus Alternaria solani. To elucidate the genomic fundament explaining these activities, we leveraged next-generation whole-genome sequencing and obtained a high-quality assembly based on short reads. The isolate bore seven known BGCs (biosynthetic gene clusters), including those responsible for producing bacillibactin, fengycin, and petrobactin. Moreover, the genome contained insecticidal genes encoding for App4Aa1, Tpp78Ba1, and Spp1Aa1 toxins, thus implicating possible pesticidal potential. We compared the genome with the 50 closest assemblies and found that b12.3 is enriched with BGCs. The genomic analysis also revealed that genomic architecture corresponds to the experimentally observed activity spectrum implying that the combination of produced secondary metabolites delineates the range of inhibited phytopathogens Therefore, this study deepens our knowledge of the biology and ecology of B. mycoides residing in the Lake Baikal region.
Assessment of Mercury Uptake by Plants in Former Cinnabar Mining Areas
Authors: Bauštein Milan , Száková Jiřina , Stefanović Luka , Najmanová Jana , Sysalová Jiřina , Tlustoš Pavel
Published: November 2024

Assessment of the plant’s ability to take up mercury (Hg) from polluted soil was affected by location, plant family, and species in two former cinnabar mining areas in the Czech Republic. At each location, seven sampling points were marked out in the vicinity of former shafts and dumpsites connected to the mining activity, where representative soil samples and dicotyledonous plants were collected. The individual locations were characterized by specific plant communities, where, in most cases, different plant species were found within one family at both locations. The total Hg content in the soil, as well as gaseous elemental mercury (GEMsoil-air), confirmed elevated levels of this element in the mining-affected environment, with high variability of the data. The low Hg accumulation ability of plants, especially the low root–shoot translocation in most of the plant species, indicated the predominant occurrence of excluders. Among the families, the results showed the exceptional position of the Fabaceae family regarding soil Hg pollution, as the highest Hg content in both shoots and roots was determined for Onobrychis viciifolia. Therefore, the behavior of Fabaceae plants in polluted soil, the mechanisms of their tolerance to high Hg content, and their Hg accumulation ability deserve further research.
Modeling, Simulation, and Control of a Rotary Inverted Pendulum: A Reinforcement Learning-Based Control Approach
Authors: Hernandez Ruben , Garcia-Hernandez Ramon , Jurado Francisco
Published: November 2024

In this paper, we address the modeling, simulation, and control of a rotary inverted pendulum (RIP). The RIP model assembled via the MATLAB (Matlab 2021a)®/Simulink (Simulink 10.3) Simscape (Simscape 7.3)™ environment demonstrates a high degree of fidelity in its capacity to capture the dynamic characteristics of an actual system, including nonlinear friction. The mathematical model of the RIP is obtained via the Euler–Lagrange approach, and a parameter identification procedure is carried out over the Simscape model for the purpose of validating the mathematical model. The usefulness of the proposed Simscape model is demonstrated by the implementation of a variety of control strategies, including linear controllers as the linear quadratic regulator (LQR), proportional–integral–derivative (PID) and model predictive control (MPC), nonlinear controllers such as feedback linearization (FL) and sliding mode control (SMC), and artificial intelligence (AI)-based controllers such as FL with adaptive neural network compensation (FL-ANC) and reinforcement learning (RL). A design methodology that integrates RL with other control techniques is proposed. Following the proposed methodology, a FL-RL and a proportional–derivative control with RL (PD-RL) are implemented as strategies to achieve stabilization of the RIP. The swing-up control is incorporated into all controllers. The visual environment provided by Simscape facilitates a better comprehension and understanding of the RIP behavior. A comprehensive analysis of the performance of each control strategy is conducted, revealing that AI-based controllers demonstrate superior performance compared to linear and nonlinear controllers. In addition, the FL-RL and PD-RL controllers exhibit improved performance with respect to the FL-ANC and RL controllers when subjected to external disturbance.
Advancements in Adenine Nucleotides Extraction and Quantification from a Single Drop of Human Blood
Authors: Popović Ivana , Dončević Lucija , Biba Renata , Košpić Karla , Barbalić Maja , Marinković Mija , Cindrić Mario
Published: November 2024

Adenine nucleotides (ANs)—adenosine 5′-triphosphate (ATP), adenosine 5′-diphosphate (ADP), and adenosine 5′-monophosphate (AMP)—are essential for energy transfer and the supply of countless processes within cellular metabolism. Their concentrations can be expressed as adenylate energy charge (AEC), a measure of cellular metabolic energy that directly correlates with the homeostasis of the organism. AEC index has broad diagnostic potential, as reduced ATP levels are associated to various conditions, such as inflammatory diseases, metabolic disorders, and cancer. We introduce a novel methodology for rapid isolation, purification, and quantification of ANs from a single drop of capillary blood. Of all the stationary phases tested, activated carbon proved to be the most efficient for the purification of adenine nucleotides, using an automated micro-solid phase extraction (µ-SPE) platform. An optimized µ-SPE method, coupled with RP-HPLC and a run time of 30 min, provides a reliable analytical framework for adenine nucleotide analysis of diverse biological samples. AN concentrations measured in capillary blood samples were 1393.1 µM, 254.8 µM, and 76.9 µM for ATP, ADP, and AMP molecules aligning with values reported in the literature. Overall, this study presents a streamlined and precise approach for analyzing ANs from microliters of blood, offering promising applications in clinical diagnostics.
Development of a New Salt of Piperine with Toluene Sulfonic Acid and Its Anti-Inflammation Effect In Vivo
Authors: Nugrahani Ilma , Sartinah Ari , Uekusa Hidehiro , Abekura Yuto , Ibrahim Slamet , Anggadiredja Kusnandar
Published: November 2024

Piperine (PPN) is a natural compound with an anti-inflammation effect and low solubility. Hence, some molecular modifications have improved its solid-state character, including cocrystal formation. However, the salt structure has yet to be widely studied. In this research, PPN was reacted with toluene sulfonic acid (TSA), which was expected to increase its solubility and anti-inflammatory effects. This experiment used solid-state reactions and analysis, including thermal analysis, infrared spectroscopy, and powder X-ray diffractometry, to characterize the new multicomponent solid phase. Next, single crystal R-ray diffractometry was used to determine the final three-dimensional conformation structure. After that, the salt, which can be reproduced by wet grinding, was tested for improved stability and anti-inflammatory effects. As a result, the PPN–TSA multicomponent solid was confirmed as a solid salt with monoclinic packing, exhibiting better solubility and anti-inflammation effects. Thus, this new organic salt has the potential for further phytochemical compound development.
Synthesis and Mesomorphic Properties of Geometric and Conformation-Modulated Amphiphilic β-Cyclodextrin Liquid Crystals
Authors: Che Austin , Ghaseminezhad Homayoun , Zellmann-Parrotta Carson O. , Duong Jessica , Williams Vance E. , Ling Chang-Chun
Published: November 2024

This study explores the liquid crystalline properties of novel amphiphilic β-cyclodextrin derivatives functionalized with seven oligoethylene glycol chains at the primary face, terminated with either an O-methyl or an O-cyanoethyl group, and fourteen hydrophobic aliphatic chains (elaidic or oleic acids) at the secondary face. These derivatives were designed to study the impact of chain conformation and terminal group polarity on their mesomorphic behavior. Thermal, microscopic, and X-ray diffraction studies revealed that the elaidic derivatives form columnar hexagonal mesophases, with the O-cyanoethyl derivative undergoing a slow, temperature-dependent transition to a bicontinuous cubic phase. The oleic derivatives, although less stable, also exhibit columnar hexagonal phases, but clear differences were observed in the clearing temperatures between these two groups of molecules, and they are also different from analogous derivatives containing no alkene functionalities. This work provides direct insights into the structure–mesomorphic property relationships of amphiphilic cyclodextrins in terms of the geometry and conformation flexibility of the hydrophobic regions, as well as the functional group attached to the end of the polar region.
Effect of Selected Organic Solvents on Hydroxyl Radical-Dependent Light Emission in the Fe2+-EGTA-H2O2 System
Authors: Sasak Krzysztof , Nowak Michał , Wlodarczyk Anna , Sarniak Agata , Nowak Dariusz
Published: November 2024

Numerous compounds that are scavengers of hydroxyl radicals (•OH) in Fenton systems have low solubility in water. Therefore, they are dissolved in organic solvents to reach suitable concentrations in the reaction milieu of the Fenton system. However, these solvents may react with •OH and iron, leading to significant errors in the results. We evaluated 11 solvents (4 alcohols, acetone, 4 esters, dimethyl-sulfoxide, and acetonitrile) at concentrations ranging from 0.105 µmol/L to 0.42 µmol/L to assess their effects on light emission, a recognized measure of •OH radical activity, in the Fe2+-EGTA-H2O2 system. Six solvents inhibited and four solvents enhanced light emission at all tested concentrations. Acetonitrile, which initially suppressed light emission, lost this effect at a concentration of 0.105 µmol/L, (−1 ± 13 (2; 0) %, p > 0.05). Methanol, at the lowest tested concentration, inhibited light emission by 62 ± 4% (p < 0.05), while butyl butyrate enhanced it by 93 ± 16% (p < 0.05). These effects may be explained by solvent-driven •OH-scavenging, inhibition or acceleration of Fe2+ regeneration, or photon emission from excited solvent molecules. Our findings suggest that acetonitrile seems suitable for preparing stock solutions to evaluate antioxidant activity in the Fe2+-EGTA-H2O2 system, provided that the final concentration of this solvent in the reaction milieu is kept below 0.105 µmol/L.
Exploration of Alkyne-Based Multilayered 3D Polymers and Oligomers: Subtle Aggregation-Induced Emission, Chromium(VI) Ion Detection, and Chiral Properties Characterization
Authors: Zhang Sai , Xu Qingzheng , Qin Xiuyuan , Mao Jialing , Zhang Yue , Li Guigen
Published: November 2024

This study investigates the synthesis and characterization of alkyne-based multilayered three-dimensional (3D) polymers, which exhibit a subtle aggregation-induced emission (AIE) phenomenon. The polymers demonstrate significant potential as fluorescent probes for the selective detection of chromium (VI) ions (Cr6⁺), showcasing their utility in environmental sensing applications. Additionally, the circular dichroism (CD) spectra reveal a pronounced cotton effect, indicative of chiral properties, while scanning electron microscopy (SEM) and dynamic light scatting (DLS) analysis reveal a distinctive rock-like surface morphology and Cr6+ sensitive anti-aggregation. These findings highlight the multifunctional capabilities of alkyne-based multilayered 3D polymers, suggesting their applicability in both fluorescence-based sensing and materials science. The insights gained from this research contribute to the development of advanced materials with tailored optical properties for environmental monitoring and other practical applications.
A New General Correlation for the Influence Parameter in Density Gradient Theory and Peng–Robinson Equation of State for n-Alkanes
Authors: Cachadiña Isidro , Hernández Ariel , Mulero Ángel
Published: November 2024

The Density Gradient Theory (DGT) permits obtaining the surface tension by using an equation of state and the so-called influence parameter. Different correlations of the influence parameter versus temperature have been proposed, with the two-coefficient ones from Zuo and Stenby (full temperature range) and Miqueu et al. (valid for the lower temperature range) being widely used. Recently, Cachadiña et al. applied the DGT with the Peng-Robinson Equation of State to esters. They proposed a new two-coefficient correlation that uses a universal exponent related to the critical exponent associated with the dependence of coexistence densities on temperature near the critical point. When applied to n-alkanes, it is shown that the Cachadiña et al. correlation must be modified to improve the lower temperature range behavior. The proposed modification results in a three-coefficient correlation that includes the triple point temperature as an input parameter and incorporates the Zuo and Stenby and Miqueu et al. correlations as particular cases. Firstly, the correlation coefficients for each of the 32 n-alkanes considered are obtained by fitting the selected values for the surface tension obtained from different databases, books, and papers. The results obtained are comparable to other specific correlations reported in the literature. The overall mean absolute percentage deviation (OMAPD) between the selected and calculated data is just 0.79%. Secondly, a general correlation with three adjustable coefficients valid for all the n-alkanes is considered. Despite the OMAPD of 4.38% obtained, this correlation is discarded due to the high deviations found for methane. Finally, it is found that a new six-coefficient general correlation, including the radius of gyration as an input fluid parameter, leads to an OMAPD of 1.78% for the fluid set considered. The use of other fluid properties as an alternative to the radius of gyration is briefly discussed.
The Effect of Combining Femtosecond Laser and Electron Irradiation on Silica Glass
Authors: Shchedrina Nadezhda , Allaoui Roqya , Sosa Matilde , Nemeth Gergely , Borondics Ferenc , Ollier Nadege , Lancry Matthieu
Published: November 2024

This study investigates the structural and optical responses of silica glass to femtosecond (fs) laser irradiation followed by high-energy electron (2.5 MeV, 4.9 GGy) irradiation. Using optical microscopy and spectroscopy techniques, we analyzed retardance, phase shifts, nanograting periodicity, and Raman D2 band intensity, which is an indicator of local glass densification. S-SNOM and nano-FTIR measurements further revealed changes in the Si–O–Si vibrational bands, indicating partial relaxation of the densified nanolayers under electron irradiation. Our findings reveal significant optical modifications due to subsequent electron irradiation, including reduced retardance and phase values, which are in agreement with the relaxation of the local densification. SEM analysis confirmed the preservation of nanogratings’ morphology including their periodicity. Apart from revealing fundamental aspects related to glass densification within nanogratings, this study also underscores the potential of combined fs-laser and electron irradiation techniques in understanding silica glass behavior under high radiation conditions, which is crucial for applications in harsh environments.
Prospects of Halide Perovskites for Solar-to-Hydrogen Production
Authors: Liu Huilong , Korukonda Tulja Bhavani , Bansal Shubhra
Published: November 2024

Solar-driven hydrogen generation is one of the promising technologies developed to address the world’s growing energy demand in an sustainable way. While, for hydrogen generation (otherwise water splitting), photocatalytic, photoelectrochemical, and PV-integrated water splitting systems employing conventional semiconductor oxides materials and their electrodes have been under investigation for over a decade, lead (Pb)- halide perovskites (HPs) made their debut in 2016. Since then, the exceptional characteristics of these materials, such as their tunable optoelectronic properties, ease of processing, high absorption coefficients, and long diffusion lengths, have positioned them as a highly promising material for solar-driven water splitting. Like in solar photovoltaics, a solar-driven water splitting field is also dominated by Pb-HPs with ongoing efforts to improve material stability and hydrogen evolution/generation rate (HER). Despite this, with the unveiling potential of various Pb-free HP compositions in photovoltaics and optoelectronics researchers were inspired to explore the potential of these materials in water splitting. In this current review, we outlined the fundamentals of water splitting, provided a summary of Pb HPs in this field, and the associated issues are presented. Subsequently, Pb-free HP compositions and strategies employed for improving the photocatalytic and/or electrochemical activity of the material are discussed in detail. Finally, this review presents existing issues and the future potential of lead-free HPs, which show potential for enhancing productivity of solar-to-hydrogen conversion technologies.
Optimization of Setpoint Conditions for Enhanced Biofabrication of Silver Nanoparticles Using Helichrysum crispum Extracts
Authors: Mphahlele Lebogang L. R. , Sekoai Patrick T. , Gbadeyan Oluwatoyin Joseph , Ramdas Veshara , Ramchuran Santosh , Chunilall Viren , Mkhize Malusi
Published: November 2024

This study investigated the optimization of setpoint conditions used for the enhanced biofabrication of silver nanoparticles (H.C-AgNPs) using Helichrysum crispum extracts. A Box–Behnken Design (BBD) model was used to evaluate the effects of reaction time, temperature, an H. crispum extraction volume, and a 0.1 M AgNO3 solution volume. A second-order polynomial regression equation was developed with a high R² of 0.9629, indicating that the model explained 96.29% of the variability in the data. The statistical significance of the model was confirmed with an F-value of 25.92 and a p-value of less than 0.0001. The optimal biofabrication conditions were determined to be a reaction time of 60 min, a temperature of 50 °C, an H. crispum extract volume of 10 mL, and a silver nitrate volume of 90 mL, achieving a peak absorbance of 3.007 a.u. The optimized conditions were experimentally validated, resulting in an absorbance of 3.386 a.u., reflecting a 12.6% increase. UV-Vis spectroscopy showed a distinct surface plasmon resonance (SPR) peak at 433 nm. XRD analysis confirmed a crystalline face-centered cubic (FCC) structure with a primary diffraction peak at 2θ = 38.44° (111 plane). SEM and EDS results confirmed a uniform size and high purity, while FTIR spectra confirmed the involvement of phytochemicals in nanoparticle stabilization. TEM analysis revealed a uniform particle size distribution with a mean size of 19.46 nm and a dispersity of 0.16%, respectively. These results demonstrate the importance of statistical tools in optimizing the setpoint conditions used in the biofabrication of AgNPs, which have applications in various fields.
Growth and Eating Behaviours at 2 Years Corrected Age in Extremely Low-Birthweight Babies; Secondary Cohort Analysis from the ProVIDe Trial
Authors: Easton Morgan J. , Bloomfield Frank H. , Jiang Yannan , Cormack Barbara E.
Published: November 2024

Early postnatal growth following extremely preterm birth may have long-term effects on growth, eating behaviours and health. Background/Objectives: To determine whether growth to age two years is conditional on growth in the NICU, a conditional growth analysis was performed in a cohort of 330 extremely low-birthweight (ELBW; birthweight < 1000 g) participants in the ProVIDe trial who were followed-up at 2 years corrected age (CA); Methods: We used z-score change for weight, length and head circumference from 36 weeks post-menstrual age to 2 years CA as the end-point-adjusted for birth z-score and z-score change from birth to 36 weeks. Growth and body composition were assessed using bioimpedance analysis. Relationships between eating behaviours and body mass index (BMI) at 2 years CA and growth were assessed using a Child Eating Behaviour Questionnaire (CEBQ) completed by parents at 2 years CA; Results: Growth, or change in z-score, from 36 weeks PMA was conditional upon growth in the NICU, with slower neonatal growth associated with faster early childhood growth (weight: R2 = 0.27, ß-coefficient −0.81 (95% CI: −0.96, −0.66), p < 0.0001; length: R2 = 0.28, ß-coefficient −0.64 (95% CI: −0.76, −0.51), p < 0.0001; head circumference: R2 = 0.18, ß-coefficient −0.61 (95% CI: −0.76, −0.46), p < 0.0001). Fat-free mass index, adjusted for confounding factors, was positively correlated with z-score change from NICU discharge to 2 years CA for weight, but not length (weight: R2 = 0.50, ß-coefficient = 0.87 (95% CI: 0.56, 1.18), p < 0.0001; length: R2 = 0.32, ß-coefficient = 0.01 (95% CI: −0.40, 0.42), p = 0.95). At 2 years CA, CEBQ scores for enjoyment were significantly higher and satiety and slowness significantly lower in children with a BMI ≥ 90th percentile than in children with a BMI ≤ 10th percentile or between the 10th−90th percentile.; Conclusions: Growth from NICU discharge to 2 years CA is conditional upon growth in the NICU, with slower NICU growth linked to faster early childhood growth, and weight z-score changes positively correlated with fat-free mass index. At age 2, children with a BMI ≥ 90th percentile have significantly different eating behaviour assessments by caregivers compared to children with a BMI ≤ 10th percentile or between the 10th–90th percentile; further RCTs are needed to confirm links between nutrition factors and growth outcomes in ELBW infants.
Impact of a Withania somnifera and Bacopa monnieri Formulation on SH-SY5Y Human Neuroblastoma Cells Metabolism Through NMR Metabolomic
Authors: D’Elia Maria , Marino Carmen , Celano Rita , Napolitano Enza , D’Ursi Anna Maria , Russo Mariateresa , Rastrelli Luca
Published: November 2024

Objectives: This study investigates the effectiveness of an herbal formulation, STRESSLESS (ST-65), which combines ashwagandha (Withania somnifera) and bacopa (Bacopa monnieri), on SH-SY5Y human neuroblastoma cells. Given the rising interest in natural compounds for neuroprotection and stress alleviation, we aimed to explore the cellular and molecular effects of this formulation. Methods: Utilizing a nuclear magnetic resonance (NMR) metabolomic approach and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS), we identified key bioactive compounds in ST-65, including withanolides from ashwagandha and bacosides from bacopa. Results: Our findings indicate that ST-65 treatment significantly alters the metabolic profile of SH-SY5Y cells. Key changes included increased levels of metabolites linked to neuroprotection, energy metabolism, and antioxidant defense. Notable enhancements were observed in specific amino acids and neuroprotective compounds, suggesting activation of neuroprotective mechanisms and mitigation of stress-induced damage. Conclusions: The study reveals a complex phyto-chemical profile of ST-65 and underscores its potential as a natural active agent for addressing stress-related neurodegenerative conditions. These insights into neuronal mechanisms provide a foundation for further exploration of herbal formulations in neuroprotection.
The Potential Role of Dietary Polyphenols in the Prevention and Treatment of Acute Leukemia
Authors: Fakhar Fatemeh , Mohammadian Kiana , Keramat Shayan , Stanek Agata
Published: November 2024

Acute leukemia is a prevalent cancer worldwide and is classified into two distinct forms. Currently, various therapies have been developed for this disease; however, the issues of recurrence, resistance to treatment, and adverse effects require the exploration of novel treatments. Polyphenols, classified into four categories, are secondary metabolites originating from plants that demonstrate diverse metabolic features such as anticancer, anti-inflammatory, and antioxidant activities. Consequently, they have attracted attention for therapeutic and preventive measures. Research indicates that dietary polyphenols can mitigate the disease burden of acute leukemias by influencing the molecular pathways associated with the disease and its inflammatory processes. Furthermore, owing to their antioxidant properties, they can reduce the amounts of reactive oxygen species generated from the disrupted molecular pathways in these malignancies. The therapeutic actions of polyphenols can facilitate disease recovery by interrupting the cell cycle and causing apoptosis by activating pro-apoptotic genes. In conclusion, the intake of dietary polyphenols, due to their convenience and availability, coupled with the positive outcomes associated with their use in conjunction with conventional therapies, may function as an efficient therapeutic and preventive measure for acute leukemia.
Daily Intake of Two or More Servings of Vegetables Is Associated with a Lower Prevalence of Metabolic Syndrome in Older People
Authors: Cubas-Basterrechea Gloria , Elío Iñaki , González Antón Carolina , Muñoz Cacho Pedro
Published: November 2024

Objectives: We sought to examine the correlation between the recommended consumption of at least two servings (400 g) of vegetables per day and the prevalence of metabolic syndrome (MetS) in an elderly population. Methods: This observational, cross-sectional, and descriptive study was conducted with 264 non-institutionalized people aged 65 to 79 years old. We adhered to the recommended guidelines for vegetable intake from the MEDAS-14 questionnaire, which has been validated for elderly populations at high cardiovascular risk. Diagnoses of MetS were made based on the criteria set forth by the International Diabetes Federation (IDF). Results: Among 264 individuals, who had a mean age of 71.9 (SD: 4.2) and comprised 39% men, the prevalence of MetS was 40.2%. A total of 17% of the participants adhered to the recommended vegetable consumption. Consuming the recommended amount of vegetables was correlated with a 19% reduction in the prevalence of MetS, to 24.4% from 43.4% among those with low vegetable consumption (p < 0.05). A main finding was that inadequate vegetable consumption was significantly associated with a higher prevalence of MetS (OR: 2.21; 95% CI: 1.06–4.63; p = 0.035), considering potential influences by nutritional (consumption of fruit and nuts) and socio-demographic (sex, age, and level of education) covariates. Conclusions: A beneficial inverse correlation was identified between the recommended vegetable intake and the prevalence of MetS. In contrast, inadequate vegetable consumption was revealed as an independent variable associated with the prevalence of MetS. Considering the very low adherence to the recommended vegetable intake we observed, encouraging increased vegetable consumption among older individuals, who have a high prevalence of MetS, is advisable.
Rosuvastatin Attenuates Vascular Dysfunction Induced by High-Fructose Diets and Allergic Asthma in Rats
Authors: Zimbru Elena-Larisa , Zimbru Răzvan-Ionuț , Ordodi Valentin-Laurențiu , Bojin Florina-Maria , Crîsnic Daniela , Andor Minodora , Mirica Silvia-Nicoleta , Huțu Ioan , Tănasie Gabriela , Haidar Laura , Nistor Daciana , Velcean Luminița , Păunescu Virgil , Panaitescu Carmen
Published: November 2024

Background: A growing body of evidence links a high-fructose diet (HFrD) to metabolic disturbances, including inflammation, dyslipidemia, insulin resistance and also endothelial dysfunction, yet its role in allergic asthma remains underexplored. Considering that obesity and hypercholesterolemia exacerbate asthma by promoting systemic inflammation, investigating interventions with dual metabolic and anti-inflammatory effects is essential. This study aimed to evaluate the potential modulatory effects of rosuvastatin in ameliorating the effects of HFrD-induced metabolic and vascular dysfunction in the context of allergic asthma. Methods: Forty-eight Sprague-Dawley rats were assigned to eight groups, receiving either a standard or HFrD for 12 weeks. Allergic asthma was induced using an ovalbumin sensitization and challenge protocol, while controls were administered saline. Selected groups were treated with rosuvastatin throughout the entire duration of the experiment. Body weight, abdominal circumference and serum biomarkers were assessed at baseline, 6 and 12 weeks. Endothelial function was assessed by evaluating vascular reactivity in an isolated organ bath. Additionally, histopathological analyses of aortic and pulmonary tissues were conducted to investigate inflammatory responses and morphological changes. Results: Rats on HFrDs exhibited significant increases in body weight, abdominal circumference, lipid profiles and blood glucose, which were further aggravated by allergic asthma. Rosuvastatin treatment notably reduced lipid levels, C-reactive protein and immunoglobulin E, while also enhancing vascular reactivity and attenuating aortic and bronchial wall thickening. Conclusions: Our findings suggest that rosuvastatin may serve as an effective therapeutic agent for addressing vascular and inflammatory complications associated with a high fructose intake and allergic asthma.
Omega-3 Fatty Acids Weaken Lymphocyte Inflammatory Features and Improve Glycemic Control in Nonobese Diabetic Goto-Kakizaki Rats
Authors: Lobato Tiago Bertola , Santos Elvirah Samantha de Sousa , Iser-Bem Patrícia Nancy , Falcão Henrique de Souza , Gimenes Gabriela Mandú , Pauferro Janaina Ribeiro Barbosa , Rodrigues Glayce Tavares , Correa Ilana Souza , Pereira Ana Carolina Gomes , Passos Maria Elizabeth Pereira , Borges João Carlos de Oliveira , Alves Amara Cassandra dos Anjos , Santos Camila Soares dos , Araújo Maria Janaina Leite de , Diniz Vinícius Leonardo Sousa , Levada-Pires Adriana Cristina , Pithon-Curi Tânia Cristina , Masi Laureane Nunes , Curi Rui , Hirabara Sandro Massao , Gorjão Renata
Published: November 2024

Background/Objectives: Goto-Kakizaki (GK) rats exhibit insulin resistance and type 2 diabetes mellitus (T2DM) without obesity. This study explored the effects of ω-3 fatty acid supplementation on T lymphocyte polarization in Wistar (WT) and GK rats. Methods: They were administered ω-3 fatty acid-rich fish oil (FO) containing eicosapentaenoic (540 mg/g) and docosahexaenoic acids (100 mg/g) by oral gavage at 2 g/kg, thrice a week for 8 weeks. The control groups (WT CT and GK CT) received the same volume of water. The following groups were investigated: GK CT, n = 14; GK ω-3, n = 15; Wistar CT, n = 15; and Wistar ω-3, n = 11. Glucose and insulin tolerance tests (GTT and ITT) were performed. Fasting plasma insulinemia and glycemia were measured. After euthanasia, the lymphocytes were extracted from the mesenteric lymph nodes. Results: The results showed that GK rats supplemented with FO had significantly improved glucose tolerance and insulin sensitivity (kITT). It also promoted greater polarization of lymphocytes toward T regulatory (Treg) features and a reduction in Th1 and Th17 profiles. Additionally, the GK ω-3 group exhibited lower cell proliferation, decreased pro-inflammatory cytokines, and increased IL-10 levels compared to the GK control. Conclusions: In conclusion, FO supplementation benefited GK rats by improving glucose intolerance, suppressing insulin resistance, and modulating lymphocytes toward Treg polarization.
Early Detection Methods for Toxic Cyanobacteria Blooms
Authors: Grant Lauren , Botelho Diane , Rehman Attiq
Published: November 2024

Harmful cyanobacterial blooms produce cyanotoxins which can adversely affect humans and animals. Without proper monitoring and detection programs, tragedies such as the loss of pets or worse are possible. Multiple factors including rising temperatures and human influence contribute to the increased likelihood of harmful cyanobacteria blooms. Current approaches to monitoring cyanobacteria and their toxins include microscopic methods, immunoassays, liquid chromatography coupled with mass spectrometry (LCMS), molecular methods such as qPCR, satellite monitoring, and, more recently, machine learning models. This review highlights current research into early detection methods for harmful cyanobacterial blooms and the pros and cons of these methods.
Investigating the Potential of Ufasomes Laden with Nintedanib as an Optimized Targeted Lung Nanoparadigm for Accentuated Tackling of Idiopathic Pulmonary Fibrosis
Authors: Aboud Heba M. , Ali Adel A. , Mohammed Nada H. , Hassan Ahmed H. E. , Roh Eun Joo , El Menshawe Shahira F.
Published: November 2024

Background/objectives: Idiopathic pulmonary fibrosis (IPF) is a prevalent interstitial lung disease that typically progresses gradually, leading to respiratory failure and ultimately death. IPF can be treated with the tyrosine kinase inhibitor, nintedanib (NTD), owing to its anti-fibrotic properties, which ameliorate the impairment of lung function. This study aimed to formulate, optimize, and assess NTD-loaded ufasomes (NTD-UFSs) as a nanosystem for its pulmonary targeting to snowball the bioavailability and therapeutic efficacy of the drug. Methods: To investigate the influence of numerous factors on NTD-UFSs assembly and to determine the optimal formulation, Box–Behnken statistical design was implemented with the assistance of Design-Expert® software. The thin-film hydration strategy was employed to fabricate NTD-UFSs. The optimum NTD-UFSs formulation was subsequently selected and subjected to additional evaluations. Also, using a rat model, a comparative pharmacokinetic analysis was scrutinized. Results: The optimal NTD-UFSs elicited an accumulative release of 65.57% after 24 h, an encapsulation efficiency of 62.51%, a zeta potential of −36.07 mV, and a vesicular size of 364.62 nm. In addition, it disclosed remarkable stability and a continuous cumulative release pattern. In vivo histopathological studies ascertained the tolerability of NTD-UFSs administered intratracheally. According to the pharmacokinetic studies, intratracheal NTD-UFSs administration manifested a significantly higher AUC0–∞ value than oral and intratracheal NTD suspensions, by approximately 5.66- and 3.53-fold, respectively. Conclusions: The findings of this study proposed that UFSs might be a promising nanoparadigm for the non-invasive pulmonary delivery of NTD.
Excitation–Emission Fluorescence Mapping Analysis of Microplastics That Are Typically Pollutants
Authors: Iqrar Syed Atif , Bibi Aisha , Chinnambedu Murugesan Raghavan , Hill Daniel , Rozhin Alex
Published: November 2024

Micro- and nanoplastics (MNPs) pose a significant threat to marine and human life due to their immense toxicity. To protect these ecosystems, the development of reliable technologies for MNP detection, characterisation, and removal is vital. While FTIR and Raman spectroscopy are established methods for MNP analysis, fluorescence (FL) spectroscopy has recently emerged as a promising alternative. However, most prior research relies on FL emission probing with a single excitation wavelength for MNP detection. In this study, we introduce a two-dimensional (2D) fluorescence excitation–emission (FLE) mapping method for the detection of commonly found microplastics, namely polystyrene (PS), polyethylene terephthalate (PET), and polypropylene (PP). The FLE mapping technique enables the collective recording of emission spectra across a range of excitation wavelengths, revealing the dominant excitation–emission features of different microplastics. This research advances the field by offering a non-destructive and label-free identification of MNP contamination through the use of FL spectral fingerprints.
Mitigation of Acid Mine Drainage Using Blended Waste Rock in Near-Equatorial Climates—Geochemical Analysis and Column Leaching Tests
Authors: Hamanaka Akihiro , Sasaoka Takashi , Shimada Hideki , Matsumoto Shinji , Kusuma Ginting Jalu , Deni Mokhamad Candra Nugraha
Published: November 2024

Acid mine drainage (AMD), wherein acidic water is generated from pyrite-containing waste rock, can be mitigated by encapsulating pyritic waste rock with cover materials to restrict the inflow of oxygen and water. However, acidic water inevitably forms during the construction of waste rock dumps before applying cover materials. Considering that the presence of waste rock containing carbonate minerals contributes to acid neutralization, a mixture of carbonate minerals and pyritic waste rock can be utilized to reduce AMD generation before the completion of the cover system as a temporary management strategy. This paper examines waste rock management using blending scenarios. Kinetic NAG and column leaching tests were employed to evaluate the blending ratio necessary to prevent acidic water generation. Geochemical analyses were conducted on rock and leachate samples, including pH and temperature measurements, XRD and XRF analyses, and Ion Chromatography. Consequently, the pH and temperature measurement results obtained during the kinetic NAG test are valuable for expressing the balance between acid generation and acid neutralization by the mixture material. Furthermore, the column leaching test demonstrated that the pH of the leachate remained neutral when the acid generation and acid neutralization reactions were well balanced. Blending waste rocks is an effective method for AMD reduction during the construction of waste rock dumps.
Salinity Stress Responses and Adaptation Mechanisms of Zygophyllum propinquum: A Comprehensive Study on Growth, Water Relations, Ion Balance, Photosynthesis, and Antioxidant Defense
Authors: Gul Bilquees , Manzoor Sumaira , Rasheed Aysha , Hameed Abdul , Ahmed Muhammad Zaheer , Koyro Hans-Werner
Published: November 2024

Zygophyllum propinquum (Decne.) is a leaf succulent C4 perennial found in arid saline areas of southern Pakistan and neighboring countries, where it is utilized as herbal medicine. This study investigated how growth, water relations, ion content, chlorophyll fluorescence, and antioxidant system of Z. propinquum change as salinity levels increase (0, 150, 300, 600, and 900 mM NaCl). Salinity increments inhibited total plant fresh weight, whereas dry weight remained constant at moderate salinity and decreased at high salinity. Leaf area, succulence, and relative water content decreased as salinity increased. Similarly, the sap osmotic potential of both roots and shoots declined as NaCl concentrations increased. Except for a transitory increase in roots at 300 mM NaCl, sodium concentrations in roots and shoots increased constitutively to more than five times higher under saline conditions than in non-saline controls. Root potassium increased briefly at 300 mM NaCl but did not respond to NaCl treatments in the leaf. Photosynthetic pigments increased with 300 and 600 mM NaCl compared to non-saline treatments, although carotenoids appeared unaffected by NaCl treatments. Except for very high NaCl concentration (900 mM), salinity showed no significant effect on the maximum efficiency of photosystem II photochemistry (Fv/Fm). Light response curves demonstrated reduced absolute (ETR*) and maximum electron transport rates (ETRmax) for the 600 and 900 mM NaCl treatments. The alpha (α), which indicates the maximum yield of photosynthesis, decreased with increasing NaCl concentrations, reaching its lowest at 900 mM NaCl. Non-photochemical quenching (NPQ) values were significantly higher under 150 and 300 mM NaCl treatments than under non-saline and higher NaCl treatments. Electrolyte leakage, malondialdehyde (MDA), and hydrogen peroxide (H2O2) peaked only at 900 mM NaCl. Superoxide dismutase and glutathione reductase activities and glutathione content in both roots and shoots increased progressively with increasing salinity. Hence, growth reduction under low to moderate (150–600 mM NaCl) salinity appeared to be an induced response, while high (900 mM NaCl) salinity was injurious.
Synergistic Effect of Biochar, Phosphate Fertilizer, and Phosphorous Solubilizing Bacteria for Mitigating Cadmium (Cd) Stress and Improving Maize Growth in Cd-Contaminated Soil
Authors: Ma Wenjun , Luo Panjun , Ahmed Sarfraz , Hayat Hafiz Saqib , Anjum Shakeel Ahmad , Nian Lili , Wu Jun , Wei Yuzhen , Ba Wenxue , Haider Fasih Ullah , Cai Liqun
Published: November 2024

Cadmium (Cd) contamination threatens human health and plant growth due to its accumulation in edible parts. The sole application of phosphorus-solubilizing bacteria (PSB), biochar (BC), and phosphorus (P) effectively mitigates Cd’s adverse effects in contaminated agricultural systems. However, further investigation into their combined impacts on Cd toxicity and maize (Zea mays) production is essential. This study evaluates the synergistic effects of PSB (10 g kg−1 of Bacillus megaterium), BC (5% w/w), and P (0.8 g kg−1) on soil properties and the morphological and physiological traits of maize cultivated in agricultural soil contaminated with Cd (20 mg kg−1). The study revealed that Cd toxicity negatively impacts soil properties, reducing shoot and root biomass, lowering chlorophyll content, and heightening oxidative stress levels. Conversely, the combined use of P, PSB, and BC markedly improved soil properties, increasing the organic matter by 175.94%, available K by 87.24%, and available P by 306.93% compared to the control. This combination also improved maize growth metrics, with increases in aboveground dry biomass (92.98%), root dry biomass (110.33%), chlorophyll a (28.20%), chlorophyll b (108.34%), and total chlorophyll (37.17%). Notably, the treatment reduced Cd concentrations in maize leaves by 61.08% while increasing soil Cd levels by 31.12% compared to the control group. Overall, the synergistic effect of P-BC-PSB is an eco-friendly strategy for mitigating Cd toxicity in contaminated soil. However, further studies are required to explore its effects and molecular mechanisms on other crops.
Leaf Spot Caused by Alternaria spp. Is a New Disease of Grapevine
Authors: Yurchenko Evgeniya , Karpova Daria , Burovinskaya Margarita , Vinogradova Svetlana
Published: November 2024

In this study, we carried out large-scale leaf spot symptom observation on vineyards in the Krasnodar Krai of Russia and determined their distribution. The incidence and severity of leaf spot were higher on the Euro-American grapevine hybrids (Bianka, Levokumskij, Avgustin, Moldova, Pervenets Magaracha, Dunavski lazur). A total of 433 isolates that belonged to the genus Alternaria were isolated from samples with leaf spot. Pathogenicity testing confirmed the ability of the representative isolates to cause necrosis on the grapevine. The isolates of Alternaria sp. were typed by the loci of internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (gapdh), Alternaria allergen a1 (Alt a1), β-tubulin (tub), and translation elongation factor (tef1). Isolates from grapevine causing leaf spot were shown to cluster with isolates of Alternaria. alternata (Fr.) Keissl. and Alternaria. arborescens E.G. Simmons species complex. Of the fungicides tested to inhibit Alternaria growth, the most effective were mixtures, such as pyrimethanil and fluopyram, cyprodinil and fludioxonil, and those that included difenoconazole. The results of the study expand our knowledge of the biodiversity of Alternaria sp. fungi and can be used to limit the spread of Alternaria leaf spot of the grapevine.
Effects of Culture Medium Enrichment with Zinc on Astaxanthin Accumulation in a New Strain of the Microalga Dysmorphococcus globosus
Authors: Zan Zhaohui , Huang Xinxin , Hussain Zahid , Zhong Moyu , Hou Chenyang , Ren Maozhi , Xie Xiulan
Published: November 2024

High Zn2+ concentrations in microalgal cells that produce astaxanthin as a feed additive can reduce the symptoms of malnutrition in aquatic animals. Therefore, in this study, we analysed the effect of Zn2+ in the culture medium on the growth of a newly isolated microalgal strain Dysmorphococcus globosus ZY24. Zn2+ and white light stress altered the pigment content in microalgal cells. In addition, high Zn2+ concentrations in the culture medium altered cell morphology and chlorophyll fluorescence and also increased intracellular Zn2+ accumulation. Further, an optimal Zn2+ concentration in the culture medium promoted the synthesis of astaxanthin and other pigments. When the concentration of Zn2+ was 45.5 mg L−1, Dysmorphococcus globosus ZY24 produced 0.31 mg g−1 astaxanthin, whereas the total zinc content of the microalgae was 4337 mg kg−1. This study confirmed that microalgae have a high capacity for Zn2+ enrichment, providing a theoretical basis for studying Zn2+ enrichment in microalgae. Furthermore, Zn2+ supplementation to stimulate astaxanthin production in microalgae is a practical method to enhance their nutritional value.
Spatio-Temporal Heterogeneity of Ecological Quality in a Typical Dryland of Northern China Driven by Climate Change and Human Activities
Authors: Li Shuai , Gao Junliang , Guo Pu , Zhang Ge , Ren Yu , Lu Qi , Bai Qinwen , Lu Jiahua
Published: November 2024

With the intensification of climate change and anthropogenic impacts, the ecological environment in drylands faces serious challenges, underscoring the necessity for regionally adapted ecological quality evaluation. This study evaluates the suitability of the original Remote Sensing Ecological Index (oRSEI), modified RSEI (mRSEI), and adapted RSEI (aRSEI) in a typical dryland region of northern China. Spatio-temporal changes in ecological quality from 2000 to 2022 were analyzed using Theil–Sen median trend analysis, the Mann–Kendall test, and the Hurst exponent. Multiple regression residual analysis quantified the relative contributions of climate change and human activities to ecological quality changes. Results showed that (1) the aRSEI was the most suitable index for the study area; (2) observed changes exhibited significant spatial heterogeneity, with improvements generally in the inner areas of the Yellow River and declines in the outer areas; and (3) changes in ecological quality were primarily driven by climate change and human activities, with human activities dominating from 2000 to 2011 and the influence of climate change increasing from 2012 to 2022. This study compares the efficacy of RSEIs in evaluating dryland ecological quality, identifies spatio-temporal change patterns, and elucidates driving mechanisms, offering scientific evidence and policy recommendations for targeted conservation and restoration measures to address future changes in dryland regions.