bluuebunny/crossref_metadata_2025 huggingface dataset:
Rosa damascena Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints
Authors: Gerasimova Tsvetelina , Gateva Svetla , Jovtchev Gabriele , Angelova Tsveta , Topashka-Ancheva Margarita , Dobreva Ana , Mileva Milka
Published: January 2025

The highly valued oil of Rosa damascena Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres of human life, obliges us to know and study the safety profile of the product obtained from the water–steam distillation of fresh rose petals. The genotoxicity of the essential oil (EsO) has not been thoroughly studied despite its wide range of applications. That predetermined the object of this study—to evaluate, through classical cytogenetic methods, the possible cytotoxic/genotoxic activities of R. damascena Mill. EsO (EsORdm) in three different test systems: plant root meristem cells, mammalian bone marrow cells, and human lymphocyte cultures. The rose essential oil showed varying concentration- and time-dependent cytotoxic and genotoxic effects depending on the test system used, and it was established that the oil showed moderate cytotoxicity in lymphocyte cultures and non-high cytotoxicity in ICR mice but none in barley. Both barley and human lymphocytes showed a genotoxic effect with a dose-dependent increase in chromosomal aberrations (CAs) and a substantial rise in micronucleus (MN) frequency, while no genotoxicity was observed in bone marrow cells at the applied concentrations. Human lymphocytes exhibited the highest susceptibility to cytotoxic and genotoxic actions of the EsO. As a valuable plant-derived aromatic product with versatile uses in human life, R. damascena Mill. essential oil should be used in an appropriate concentration range tailored to cellular sensitivity.
Electrical Properties of Cu-Based Coordination Complexes: Insights from In Situ Impedance Spectroscopy
Authors: Pisk Jana , Dunatov Marko , Stojić Martina , Judaš Nenad , Đilović Ivica , Razum Marta , Pavić Luka
Published: January 2025

This study examines the influence of ligand design on the structural, optical, and electrical properties of copper-based coordination complexes. Ligands H2L1 and H2L2 were synthesized via the reaction of 5-nitrosalicylaldehyde with 2-hydroxy- or 4-hydroxybenzhydrazide. H4L3 was obtained from the reaction of carbohydrazide and salicylaldehyde, while H4L4 was prepared by condensing 4-methoxysalicylaldehyde with thiocarbohydrazide. The research focuses on two key design elements: (1) the effect of hydroxyl group positioning on the aroyl ring in hydrazide ligands (H2L1 vs. H2L2) and (2) the impact of carbonyl versus thiocarbonyl groups and aldehyde substituents in hydrazone ligands (H4L3 vs. H4L4). The resulting complexes, [Cu2(L1)2], [Cu2(L2)2(MeOH)3], [Cu2(L3)(H2O)2], and [Cu2(L4)(H2O)2], were synthesized and characterized using attenuated total reflectance infrared (IR-ATR) spectroscopy, thermogravimetric analysis (TG), and UV-Vis diffuse reflectance spectroscopy. Their electrical properties were investigated using solid-state impedance spectroscopy (IS). The crystal and molecular structure of the complex [Cu2(L2)2(MeOH)3]∙MeOH was determined by single-crystal X-ray diffraction (SCXRD). This study underscores the pivotal role of ligand modifications in modulating the functional properties of coordination complexes, offering valuable insights for the advancement of materials chemistry.
Mechanistic Aspects of [3+2] Cycloaddition Reaction of Trifluoroacetonitrile with Diarylnitrilimines in Light of Molecular Electron Density Theory Quantum Chemical Study
Authors: Łapczuk Agnieszka , Ríos-Gutiérrez Mar
Published: January 2025

In this study, we investigated the [3+2] cycloaddition reaction of CF3CN (TFAN) with nitrilimine (NI) to produce 1,2,4-triazole and compared the resulting isomers. We determined the preferred reaction pathway by examining the electrophilic and nucleophilic properties of the reaction substrates, performing thermodynamic calculations for the individual pathways, and comparing them with the experimental results.
Study of the Effect of Methyl Eugenol on Gastric Damage Produced by Spinal Cord Injury Model in the Rat
Authors: Cruz-Antonio Leticia , Sánchez-Mendoza María Elena , García-Machorro Jazmín , López-Lorenzo Yaraset , Arrieta Jesús
Published: January 2025

Traumatic spinal cord injury (SCI) is a serious medical condition that places patients at high risk of developing gastric ulceration and gastrointestinal bleeding. One preventative strategy involves the use of omeprazole; however, its chronic use is associated with adverse effects, highlighting the need for alternative therapies. This study evaluated the protective effects of methyl eugenol (ME) on gastric mucosal damage in a rat model of SCI. ME was administered orally at doses of 30, 100, and 177 mg/kg in SCI induced at the T9 level, alongside diclofenac or ketorolac (30 mg/kg each). The enzymatic activity of superoxide dismutase, catalase, and glutathione peroxidase was assessed, and the levels of total glutathione and malondialdehyde were determined using biochemical kits. Additionally, stomach histological sections were analyzed. ME exhibited dose-dependent gastroprotective effects, with maximal protection observed at 177 mg/kg in the presence of diclofenac (9.78 ± 2.16 mm2) or ketorolac (12.49 ± 2.17 mm2). A histological analysis confirmed these findings. In conclusion, methyl eugenol protects the gastric mucosa from SCI-induced damage, with glutathione peroxidase and catalase playing key roles in its mechanism of gastroprotection.
The Olive Oil Monophenolic Secoiridoid Ligstroside Aglycone Suppresses Melanoma Progression by Targeting the BRAF Signaling Pathway
Authors: Mahmud Md Ashiq , Siddique Abu Bakar , Tajmim Afsana , King Judy Ann , El Sayed Khalid A.
Published: January 2025

Melanoma is among the most abundant malignancies in the US and worldwide. Ligstroside aglycone (LA) is a rare extra-virgin olive oil-derived monophenolic secoiridoid with diverse bioactivities. LA dose–response screening at the NCI 60 cancer cells panel identified the high sensitivity of the Malme-3M cell line, which harbors a BRAF V600E mutation. Daily oral 10 mg/kg LA exhibited potent in vivo antitumor effects against Malme-3M cells xenograft in a nude mouse model by targeting the BRAF signaling pathway. A human Clariom S microarray analysis of the collected Malme- 3M tumors identified 571 dysregulated genes, with the downregulation of pathways critical for melanoma cells growth and survival. A Western blot analysis of the collected animal tumors further validated the downregulation of the mutated BRAF–MAPK axis, as well as the GPD1 and ELOVL6 expression levels. A histopathological analysis of Malme-3M tumor sections showed extensive focal tumor necrosis in treated mice. An immunofluorescence study of tumor sections showed notable reductions in proliferation marker ki67 and the vasculogenesis marker CD31 in treated tumors. These findings promote LA as a potential nutraceutical lead for the control of the BRAF V600E mutant melanoma.
Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry
Authors: Tuzimski Tomasz , Szubartowski Szymon , Stążka Janusz , Baczewski Kamil , Janiszewska Daria , Railean Viorica , Buszewski Bogusław , Szultka-Młyńska Małgorzata
Published: January 2025

Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values in the range of 62%–98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC–MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time.
Investigation of the Catalytic Properties of Aluminum Oxide (Al2O3) and Pyrite (FeS2) Using Thermodynamic and Kinetic Parameters
Authors: Ordabaeva Aigul T. , Muldakhmetov Zainulla M. , Meiramov Mazhit G. , Kim Sergey V. , Kasenova Shuga B. , Fazylov Serik D.
Published: January 2025

The kinetics of anthracene hydrogenation was studied using the method of equilibrium kinetic analysis. To determine the diffusion–kinetic characteristics, anthracene hydrogenation was performed at different temperatures (648 K, 673 K, 698 K), at a hydrogen pressure of 3 MPa in the presence of a mixture of pyrite (FeS2) and aluminum oxide (Al2O3) taken at a ratio of 1:1. Chromatographic analysis of anthracene hydrogenation products showed the presence of 9,10-dihydroanthracene (DHA), 1,2,3,4-tetrahydroanthracene (THA), methylnaphthalene (MN), naphthalene (H) and other unidentified compounds. In order to preserve the material balance, a total hydrogenation reaction of anthracene, up to 9,10-dihydroanthracene, was proposed as characterized by the highest rate in the presence of pyrite-based catalysts and aluminum oxide. Calculations of the degrees of rotation of anthracene, reaction constants, and Gibbs energy have shown that with increasing temperature, the reaction becomes more thermodynamically advantageous. Based on the obtained data, Arrhenius dependences were constructed, which made it possible to calculate the activation energies of direct (39.4 kJ/mol) and reverse (13.04 kJ/mol) reactions. Thus, based on the calculations performed, it was found that the process of anthracene hydrogenation in the presence of a mixture of pyrite and aluminum oxide proceeds mainly in the diffusion region.
Maternal Lifestyle Factors Affecting Breast Milk Composition and Infant Health: A Systematic Review
Authors: Favara Giuliana , Maugeri Andrea , Barchitta Martina , Lanza Erika , Magnano San Lio Roberta , Agodi Antonella
Published: January 2025

Background/Objectives: Breast milk is a dynamic, personalized nutrition source, influenced by maternal diet, lifestyle, and environmental factors, which shape its composition and impact infant health. This review synthesizes evidence on the associations between maternal lifestyles (e.g., diet, physical activity, smoking), breast milk composition, and child health, offering insights for interventions to optimize breastfeeding benefits. Methods: We searched Web of Science, Medline, Embase, and PubMed for studies published up to March 2024 using predefined terms. Results: Out of 5244 articles, 20 studies met the inclusion criteria. Maternal Body Mass Index and macronutrient intake significantly affected breast milk fatty acid composition, influencing infant growth, cognitive development, and metabolic health. Micronutrient intake, particularly iodine, omega-3 fatty acids, and vitamins, was linked to better neurodevelopment and reduced atopic risks. Maternal diet and supplementation improved breast milk nutrient profiles and infant outcomes, though exposure to toxins like ochratoxin A raised concerns. Smoking was associated with altered milk composition, including lower osteopontin levels, potentially affecting infant immunity and growth. Conclusions: This review emphasizes that adequate intake of key nutrients is essential for infant development, highlighting the need for policies that address nutritional deficiencies, promote healthy lifestyles, and reduce socio-economic barriers. These efforts can improve outcomes for both mothers and children, enhancing public health and reducing disparities.
Maternal Long-Chain Polyunsaturated Fatty Acids Status in Pregnancy and Newborn Body Composition
Authors: Dewi Mira , Andarwulan Nuri , Wahyuningsih Utami , Kazimierczak Renata , Średnicka-Tober Dominika
Published: January 2025

Background: A number of clinical studies have shown a positive association between the maternal n-3 PUFA status during pregnancy and fetal and newborn development and health. Despite this well-documented role of n-3 PUFAs in pregnancy, data on maternal the LC-PUFAs status during pregnancy in the Indonesian population, to our knowledge, are not yet available. This study reports on the LC-PUFA dietary intake among pregnant women in a suburban population of Bogor City, West Java, Indonesia. It also explores the associations of maternal LC-PUFA intake with maternal blood, cord blood, and breast milk LC-PUFA levels and the associations of the latter with infant body composition. Methods: A total of 142 pregnant women and 104 newborn infants were included in this study. The dietary intake of energy, macronutrients, and selected LC-PUFAs (LA, ALA, EPA, and DHA) was assessed by 2 × 24 h food recall and FFQ. LC-PUFA levels were measured in maternal blood, cord blood, and breast milk. Newborn body composition was determined by anthropometric measures. Results: The study found that the inadequate intake of energy, protein, and carbohydrates was highly prevalent among pregnant women subjects. The intake of the most important n-3 PUFAs (ALA, EPA, and DHA) was far below the recommended values. Maternal dietary DHA intakes were negatively associated with birth weight and infant fat mass at birth, and dietary intake of total fat and n-6 LA were associated with increased fat accumulation in newborns at specific body sites. Moreover, positive correlations were identified between the EPA in maternal blood and infant % fat mass, and between the DHA in cord blood and newborn birth weight. Conclusions: Further longitudinal studies, including clinical and biomolecular analyses, are suggested to be conducted to monitor maternal and child health and nutrition in Indonesia and develop well-attuned intervention strategies.
Weight-Adjusted Waist Index (WWI)—A Promising Anthropometric Indicator of Depressive Symptoms in Hospitalized Older Patients
Authors: Korycka-Bloch Renata , Balicki Pawel , Guligowska Agnieszka , Soltysik Bartlomiej K. , Kostka Tomasz , Chrzastek Zuzanna
Published: January 2025

Objectives: The aim of this study was to evaluate which anthropometric index, either body mass index (BMI) or weight-adjusted waist index (WWI), is more accurately associated with the prevalence of the most common chronic diseases and components of geriatric assessment in hospitalized older adults. Methods: The study included a total of 2945 hospitalized older adults (median age 82 years). The associations between the presence of chronic diseases and Comprehensive Geriatric Assessment (CGA) results were compared with WWI and BMI values. Results: The WWI was significantly higher in both sex groups suffering from hypertension, diabetes, osteoarthritis, and depression. In women, the parameter was increased among individuals with previous myocardial infarction, who presented heart failure symptoms or had chronic kidney disease diagnosed, whereas in men, among those with pulmonary diseases and osteoporosis, WWI was related to many CGA parameters oftentimes where BMI proved to fail. There was a positive correlation of WWI with the presence of depressive symptoms assessed with the geriatric depression scale (GDS) but no significant correlation with BMI. In multiple logistic regression models, WWI was a stronger predictor of depression as compared to waist circumference or the waist-to-height ratio. Conclusions: There is an association between a higher WWI and depression diagnosis as well as the presence of depressive symptoms according to the GDS in hospitalized older adults, both women and men. There is no such correlation between depression and BMI. Both high BMI and high WWI values seem to identify older patients with cardiometabolic diseases such as hypertension and diabetes. According to this study, WWI seems to be a promising indicator of depression risk and, similarly to BMI, a useful parameter for the assessment of cardiometabolic risk in older hospitalized adults.
Impacts of Diabetes Self-Management Education and Support Programs in Hispanic Church Settings: A Cluster-Randomized Trial Comparing Faith-Based and Faith-Placed Approaches
Authors: Wilmoth Summer R. , Carrillo-McCracken Leah L. , Wilhite Bradley , Pan Meixia , Parra-Medina Deborah , Sosa Erica T. , Reyes Ramon , He Meizi
Published: January 2025

Background/Objectives: This study aimed to adapt evidence-based diabetes self-management education and support (DSMES) into a faith-based (FB) context for Hispanic communities and compare its effectiveness to a faith-placed (FP) approach using the church as a venue for DSMES delivery. Methods: A cluster-randomized trial was conducted among adults with type 2 diabetes from predominantly Hispanic churches. The churches were assigned to either the FB Group (nine churches, n = 146) or the FP Group (seven churches, n = 125). The FB Group, led by trained lay health leaders, received a health sermon, a six-session DSMES program, and a seven-session Healthy Bible Study. The FP Group, led by outside health professionals, received the same six-session DSMES and a seven-session partial attention control curriculum. Key outcomes, including hemoglobin A1c (HbA1c), waist circumference (WC), diabetes distress, self-care activities, and self-efficacy, were assessed at baseline, 6, 9, and 12 months. Results: The FB Group had lower HbA1c levels than the FP Group at 6 months (−0.3%, p < 0.01), with no within-group differences post-intervention. No significant between-group differences were found for other outcomes. Within-group comparisons from baseline showed that both groups reduced WC at 9 and 12 months. Both groups showed reductions in diabetes distress and increased self-efficacy at all time points post-intervention (p < 0.05). The FB Group increased self-care scores at all time points post-intervention, while the FP Group increased at 9 and 12 months. Conclusions: DSMES can be effectively delivered in church settings by trained lay leaders or health professionals in Hispanic communities. Adding a spiritual dimension to DSMES may enhance outcomes.
Role of Increasing Body Mass Index in Gut Barrier Dysfunction, Systemic Inflammation, and Metabolic Dysregulation in Obesity
Authors: Maqoud Fatima , Calabrese Francesco Maria , Celano Giuseppe , Mallardi Domenica , Goscilo Francesco , D’Attoma Benedetta , Ignazzi Antonia , Linsalata Michele , Bitetto Gabriele , Di Chito Martina , Pesole Pasqua Letizia , Diciolla Arianna , Apa Carmen Aurora , De Pergola Giovanni , Giannelli Gianluigi , De Angelis Maria , Russo Francesco
Published: January 2025

Aims: This study explores the link between body mass index (BMI), intestinal permeability, and associated changes in anthropometric and impedance parameters, lipid profiles, inflammatory markers, fecal metabolites, and gut microbiota taxa composition in participants having excessive body mass. Methods: A cohort of 58 obese individuals with comparable diet, age, and height was divided into three groups based on a priori clustering analyses that fit with BMI class ranges: Group I (25–29.9), Group II (30–39.9), and Group III (>40). Anthropometric and clinical parameters were assessed, including plasma C-reactive protein and cytokine profiles as inflammation markers. Intestinal permeability was measured using a multisaccharide assay, with fecal/serum zonulin and serum claudin-5 and claudin-15 levels. Fecal microbiota composition and metabolomic profiles were analyzed using a phylogenetic microarray and GC-MS techniques. Results: The statistical analyses of the clinical parameters were based on the full sample set, whereas a subset composed of 37 randomized patients was inspected for the GC/MS metabolite profiling of fecal specimens. An increase in potentially pro-inflammatory bacterial genera (e.g., Slackia, Dorea, Granulicatella) and a reduction in beneficial genera (e.g., Adlercreutzia, Clostridia UCG-014, Roseburia) were measured. The gas chromatography/mass spectrometry analysis of urine samples evidenced a statistically significant increase in m-cymen-8-ol, 1,3,5-Undecatriene, (E, Z) and a decreased concentration of p-cresol, carvone, p-cresol, and nonane. Conclusions: Together, these data demonstrated how an increased BMI led to significant changes in inflammatory markers, intestinal barrier metabolites, glucose metabolism, endocrine indicators, and fecal metabolomic profiles that can indicate a different metabolite production from gut microbiota. Our findings suggest that targeting intestinal permeability may offer a therapeutic approach to prevent and manage obesity and related metabolic complications, reinforcing the link between gut barrier function and obesity.
Mechanistic and Therapeutic Insights into Flavonoid-Based Inhibition of Acetylcholinesterase: Implications for Neurodegenerative Diseases
Authors: Cichon Natalia , Grabowska Weronika , Gorniak Leslaw , Stela Maksymilian , Harmata Piotr , Ceremuga Michal , Bijak Michal
Published: January 2025

Flavonoids are naturally occurring polyphenolic compounds known for their extensive range of biological activities. This review focuses on the inhibitory effects of flavonoids on acetylcholinesterase (AChE) and their potential as therapeutic agents for cognitive dysfunction. AChE, a serine hydrolase that plays a crucial role in cholinergic neurotransmission, is a key target in the treatment of cognitive impairments due to its function in acetylcholine hydrolysis. Natural polyphenolic compounds, particularly flavonoids, have demonstrated significant inhibition of AChE, positioning them as promising alternatives or adjuncts in neuropharmacology. This study specifically examines flavonoids such as quercetin, apigenin, kaempferol, and naringenin, investigating their inhibitory efficacy, binding mechanisms, and additional neuroprotective properties, including their antioxidant and anti-inflammatory effects. In vitro, in vivo, and in silico analyses reveal that these flavonoids effectively interact with both the active and peripheral anionic sites of AChE, resulting in increased acetylcholine levels and the stabilization of cholinergic signaling. Their mechanisms of action extend beyond mere enzymatic inhibition, as they also exhibit antioxidant and anti-amyloidogenic properties, thereby offering a multifaceted approach to neuroprotection. Given these findings, flavonoids hold considerable therapeutic potential as modulators of AChE, with implications for enhancing cognitive function and treating neurodegenerative diseases. Future studies should prioritize the enhancement of flavonoid bioavailability, evaluate their efficacy in clinical settings, and explore their potential synergistic effects when combined with established therapies to fully harness their potential as neurotherapeutic agents.
Collegiate Marching Band Artists Self-Report Previous Exercise-Associated Hyponatremia: A Cross-Sectional Study
Authors: Emerson Dawn M. , Kelly Melani R. , Torres-McGehee Toni M. , Uriegas Nancy A. , Smith Matthew O. , Smith Allison B. , Kloesel Kevin A.
Published: January 2025

Exercise-associated hyponatremia (EAH) is commonly observed in endurance athletes, where prolonged physical exertion combined with being unaware of personal hydration needs can lead to excessive water consumption or inadequate sodium intake. Marching band (MB) is an emerging setting for sports medicine professionals. However, there is little research on non-musculoskeletal illnesses among these performing artists. This study is part of a larger cross-sectional study and seeks to examine whether MB artists have previously experienced EAH and the presence of known EAH risk factors (e.g., pre-existing medical conditions, medication use, nutritional behaviors). Active collegiate MB members (n = 1207; mean age = 19.6 ± 1.3 years) completed an online survey that characterized demographics, medical history, and nutrition behaviors. Statistical analyses included descriptives (mean, standard deviation) and frequencies for all data. Binomial logistic regressions analyzed the relationship and determined the odds ratio (OR) between previous EAH and EAH risk factors. Participants were from 23 different states, predominately White (82.9%), female (56.7%), and at NCAA Division I institutions (92%). A total of 74 (6.1%) participants reported previously experiencing EAH, 32 (43.2%) within the past year. Previous EAH occurrence was greatest among individuals who had kidney conditions (χ2(1) = 5.920, R2 = 0.013, OR = 15.708, p = 0.015); mood/neurological conditions (χ2(1) = 7.508, R2 = 0.017, OR = 2.154, p = 0.006), particularly anxiety (χ2(1) = 7.651, R2 = 0.017, OR = 3.590, p = 0.006); used mental health medications (χ2(1) = 7.512, R2 = 0.017, OR = 2.220, p = 0.006), particularly selective serotonin reuptake inhibitors (χ2(1) = 13.502, R2 = 0.030, OR = 3.297, p < 0.001); and who dieted (χ2(1) = 6.121, R2 = 0.014, OR = 1.913, p = 0.013) or perceived they had an eating disorder (χ2(1) = 9.502, R2 = 0.021, OR = 2.729, p = 0.002). Healthcare providers should be aware that MB artists may have pre-existing medication conditions, use medication, and have inadequate nutrition. Targeted hydration and nutritional education for MB artists is essential for effective prevention and early EAH recognition, improving health and performance for MB artists within demanding environments.
Individual and Joint Associations Between Animal and Plant Protein Intakes with Impaired Fasting Glucose and Type 2 Diabetes in the Framingham Offspring Study
Authors: Pickering R. Taylor , Yiannakou Ioanna , Lara-Castor Laura , Bradlee M. Loring , Singer Martha R. , Moore Lynn L.
Published: January 2025

Objectives: Given the considerable discrepancy in the literature regarding dietary protein and glucose homeostasis, we examined the prospective association between protein intake (total, animal, plant) and risk of type 2 diabetes mellitus or impaired fasting glucose (IFG). We also examined whether these associations differed by sex, body weight, or other risk factors. Methods: We included 1423 subjects, aged ≥ 30 years, in the Framingham Offspring Study cohort. Three-day dietary records at exams 3 and 5 were used to average protein intake and then adjusted for body weight residuals. Cox proportional hazard models were used to estimate hazard ratios (HR), adjusting for anthropometric, demographic, and lifestyle factors over ~16 years of follow-up. Results: Subjects with the highest total protein intakes (≥100 g men; ≥85 g women) had a 31% lower risk of type 2 diabetes/IFG (95% CI: 0.54, 0.87). The highest (vs. lowest) category of intake of animal protein was associated with a 32% lower risk of diabetes/IFG (95% CI: 0.55, 0.83), whereas plant protein was not. Beneficial trends of total protein, especially animal, were stronger for women (HR: 0.61; 95% CI: 0.42, 0.87) than for men (HR: 0.82; 95% CI 0.58, 1.15). Subjects with lower BMI who consumed more protein had the lowest risks of diabetes/IFG. Conclusions: Overall, in this prospective study, higher intake of total dietary protein, including the consumption of animal protein, particularly among individuals with lower BMI and higher physical activity levels, was inversely associated with risk of incident type 2 diabetes and IFG.
High-Intensity Interval Training Reduces Liver Enzyme Levels and Improves MASLD-Related Biomarkers in Overweight/Obese Girls
Authors: Abassi Wissal , Ouerghi Nejmeddine , Hammami Mohamed Bessem , Jebabli Nidhal , Feki Moncef , Bouassida Anissa , Weiss Katja , Knechtle Beat
Published: January 2025

Background/Objectives: Despite the abundant body of evidence linking high-intensity interval training (HIIT) to cardiometabolic markers, little is known about how HIIT affects liver enzymes, particularly in obese adolescents. This study aimed to investigate the effects of HIIT on metabolic dysfunction-associated steatotic liver disease (MASLD)-related biomarkers in overweight/obese adolescent girls. Methods: Thirty-three overweight/obese adolescent girls (age, 17.0 ± 1.15 yr.; body mass index, 33.3 ± 4.77 kg/m2) were randomly assigned to HIIT (n = 17) or control (n = 16) groups. The HIIT group participated in a nine-week HIIT program (three times weekly) without caloric restriction. Maximal aerobic speed, body composition indexes, blood pressure, MASLD-related biomarkers [liver enzymes (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)), plasma lipids, uric acid, platelet count, and homeostasis model assessment index for insulin-resistance (HOMA-IR)] were examined at baseline and after the intervention. Results: Significant “time × group” interactions were found for body composition indexes, systolic blood pressure, maximal aerobic speed, liver enzymes ALT and AST, plasma lipids, glucose, and HOMA-IR. The HIIT program resulted in an increase in maximal aerobic speed (p = 0.035) and a decrease in body composition and plasma lipids (p < 0.01), systolic blood pressure (p = 0.011), ALT (p = 0.013), AST (p = 0.012), and HOMA-IR (p = 0.01), but no significant changes in uric acid and platelet count. None of these markers changed in the control group. Conclusions: HIIT resulted in an improvement in MASLD-related biomarkers. HIIT could be an effective exercise therapy to prevent and reverse MASLD in adolescents with obesity.
The Association Between the Dietary Inflammatory Index, Dietary Pattern, and Hypertension Among Residents in the Xinjiang Region
Authors: Wang Min , Liao Jiali , Wang Hao , Deng Lu , Zhang Tingyu , Guo Heng , Qian Xin , Ma Rulin
Published: January 2025

Background: Diet and inflammation are both associated with hypertension. We aimed to investigate the relationship between the dietary inflammation index (DII), dietary patterns, and the risk of hypertension among Xinjiang residents. Methods: A total of 930 residents aged 20–80 from Shihezi and Tumushuk were selected as participants using a stratified whole cluster random sampling method. General demographic information, dietary data, and physical examination results were collected from the participants and DII scores were calculated. Restricted cubic spline was used to analyze the dose–response relationship between the DII and the risk of hypertension. LASSO regression was used to screen dietary factors associated with hypertension. Factor analysis was used to extract dietary patterns. Finally, logistic regression modeling was used to analyze the association between the DII, dietary patterns, and the risk of hypertension. Results: The DII was linearly and positively associated with the risk of developing hypertension. Logistic regression analysis showed that the prevalence of hypertension was 2.23 (95% CI: 1.53, 3.23) and 3.29 (95% CI: 2.26, 4.79) in the T2 and T3 groups, respectively, compared with the T1 group. Riboflavin and folate were associated with the risk of hypertension. In the vegetable–egg dietary pattern, the risk of hypertension was reduced by 33%, 39%, and 37% in groups Q2, Q3, and Q4, respectively, compared with group Q1 (Q2: OR = 0.67, 95% CI: 0.45, 0.99; Q3: OR = 0.61, 95% CI: 0.41, 0.92; Q4: OR = 0.63, 95% CI: 0.42, 0.96). Conclusions: The higher the DII score, the higher the risk of hypertension among residents of Xinjiang. In addition, vegetable–egg dietary patterns can reduce the risk of hypertension. Therefore, local residents should be scientifically instructed to increase their intake of vegetables and eggs.
Validation of a Questionnaire Assessing Pregnant Women’s Perspectives on Addressing the Psychological Challenges of Childbirth
Authors: Radu Mihaela Corina , Armean Mihai Sebastian , Chivu Razvan Daniel , Aurelian Justin , Pop-Tudose Melania Elena , Manolescu Loredana Sabina Cornelia
Published: January 2025

Introduction: Pregnant women’s experiences and concerns regarding childbirth are complex, necessitating a multidimensional and personalized approach in maternal care. This study explores the psychological and emotional factors influencing pregnant women’s decisions regarding their mode of delivery. The results will provide valuable insights for the development of educational and counseling strategies designed to support pregnant women in making informed and conscious decisions about their childbirth. Material and method: This cross-sectional study aimed to develop and validate a questionnaire exploring the psychological dimensions of childbirth. Factor analysis was employed to assess emotional perceptions, perceived medical risks, and the impact of cesarean section on pregnant women. The questionnaire was distributed online via Google Forms, using social networks like Facebook and Instagram to ensure rapid and broad accessibility. The questionnaire was available for seven months, from January to July 2023. Results: McDonald’s ω, Cronbach’s α, average inter-item correlation, and total item correlations were calculated to assess the consistency of the questionnaire items in measuring the same construct. The three-factor model emerged as the primary structure based on exploratory and confirmatory factor analyses (EFA and CFA). The first profile, centered on the psychological and emotional benefits of vaginal birth, highlights the importance of the natural birth experience for the mother’s psychological well-being. The second profile addresses concerns about medical risks and the need for interventions. The third profile focuses on perceptions and concerns related to the intelligence and adaptability of children born by cesarean section and the effects of anesthesia. Conclusions: Each profile reflects different strategies for seeking control and security amid childbirth uncertainties. These include emphasizing the psychological benefits of vaginal birth, addressing medical risks, and focusing on the impact of interventions on child development. Understanding these variables is essential for providing appropriate counseling and psychosocial support, thereby optimizing the birth experience and promoting the health of both mother and child. The integration of multi-factor and single-factor models in the questionnaire analysis serves complementary purposes, providing distinct yet interrelated insights into the instrument’s structure and validity.
Astrocytic HIV-1 Nef Expression Decreases Glutamate Transporter Expression in the Nucleus Accumbens and Increases Cocaine-Seeking Behavior in Rats
Authors: Pla-Tenorio Jessalyn , Velazquez-Perez Bethzaly , Mendez-Borrero Yainira , Cruz Myrella L. , Sepulveda-Orengo Marian T. , Noel Richard J.
Published: January 2025

Background/Objectives: Cocaine use disorder is an intersecting issue in populations with HIV-1, further exacerbating the clinical course of the disease and contributing to neurotoxicity and neuroinflammation. Cocaine and HIV neurotoxins play roles in neuronal damage during neuroHIV progression by disrupting glutamate homeostasis in the brain. Even with combined antiretroviral therapy (cART), HIV-1 Nef, an early viral protein expressed in approximately 1% of infected astrocytes, remains a key neurotoxin. This study investigates the relationship among Nef, glutamate homeostasis, and cocaine in the nucleus accumbens (NAc), a critical brain region associated with drug motivation and reward. Methods: Male and female Sprague Dawley rats were used to compare the effects of astrocytic Nef and cocaine by molecular analysis of glutamate transporters, GLT-1 and the cysteine glutamate exchanger (xCT), in the NAc. Behavioral assessments for cocaine self-administration were used to evaluate cocaine-seeking behavior. Results: The findings indicate that both cocaine and Nef independently decrease the expression of the glutamate transporter GLT-1 in the NAc. Additionally, rats with astrocytic Nef expression exhibited increased cocaine-seeking behavior but demonstrated sex-dependent molecular differences after the behavioral paradigm. Conclusions: The results suggest that the expression of Nef intensifies cocaine-induced alterations in glutamate homeostasis in the NAc, potentially underlying increased cocaine-seeking behavior. Understanding these interactions better may inform therapeutic strategies for managing cocaine use disorder in HIV-infected individuals.
Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication
Authors: Lezzhov Alexander A. , Atabekova Anastasia K. , Chergintsev Denis A. , Lazareva Ekaterina A. , Solovyev Andrey G. , Morozov Sergey Y.
Published: January 2025

Among the long non-coding RNAs that are currently recognized as important regulatory molecules influencing a plethora of processes in eukaryotic cells, circular RNAs (circRNAs) represent a distinct class of RNAs that are predominantly produced by back-splicing of pre-mRNA. The most studied regulatory mechanisms involving circRNAs are acting as miRNA sponges, forming R-loops with genomic DNA, and encoding functional proteins. In addition to circRNAs generated by back-splicing, two types of circRNAs capable of autonomous RNA-RNA replication and systemic transport have been described in plants: viroids, which are infectious RNAs that cause a number of plant diseases, and retrozymes, which are transcripts of retrotransposon genomic loci that are capable of circularization due to ribozymes. Based on a number of common features, viroids and retrozymes are considered to be evolutionarily related. Here, we provide an overview of the biogenesis mechanisms and regulatory functions of non-replicating circRNAs produced by back-splicing and further discuss in detail the currently available data on viroids and retrozymes, focusing on their structural features, replication mechanisms, interaction with cellular components, and transport in plants. In addition, biotechnological approaches involving replication-capable plant circRNAs are discussed, as well as their potential applications in research and agriculture.
Genome-Wide Identification and Expression Profiling of the SPL Transcription Factor Family in Response to Abiotic Stress in Centipedegrass
Authors: Kong Dandan , Xu Maotao , Liu Siyu , Liu Tianqi , Liu Boyang , Wang Xiaoyun , Dong Zhixiao , Ma Xiao , Zhao Junming , Lei Xiong
Published: January 2025

SQUAMOSA promoter-binding protein-like (SPL) transcription factors play a critical role in the regulation of gene expression and are indispensable in orchestrating plant growth and development while also improving resistance to environmental stressors. Although it has been identified across a wide array of plant species, there have been no comprehensive studies on the SPL gene family in centipedegrass [Eremochloa ophiuroides (Munro) Hack.], which is an important warm-season perennial C4 turfgrass. In this study, 19 potential EoSPL genes in centipedegrass were identified and assigned the names EoSPL1-EoSPL19. Gene structure and motif analysis demonstrated that there was relative consistency among the branches of the phylogenetic tree. Five pairs of segmental duplication events were detected within centipedegrass. Ten EoSPL genes were predicted to be targeted by miR156. Additionally, the EoSPL genes were found to be predominantly expressed in leaves and demonstrated diverse responses to abiotic stress (salt, drought, glufosinate ammonium, aluminum, and cold). This study offers a comprehensive insight into the SPL gene family in centipedegrass, creating a foundation for elucidating the functions of EoSPL genes and investigating their involvement in abiotic stress responses.
Combined Effects of Microgravity and Chronic Low-Dose Gamma Radiation on Brassica rapa Microgreens
Authors: De Francesco Sara , Le Disquet Isabel , Pereda-Loth Veronica , Tisseyre Lenka , De Pascale Stefania , Amitrano Chiara , Carnero Diaz Eugénie , De Micco Veronica
Published: January 2025

Plants in space face unique challenges, including chronic ionizing radiation and reduced gravity, which affect their growth and functionality. Understanding these impacts is essential to determine the cultivation conditions and protective shielding needs in future space greenhouses. While certain doses of ionizing radiation may enhance crop yield and quality, providing “functional food” rich in bioactive compounds, to support astronaut health, the combined effects of radiation and reduced gravity are still unclear, with potential additive, synergistic, or antagonistic interactions. This paper investigates the combined effect of chronic ionizing radiation and reduced gravity on Brassica rapa seed germination and microgreens growth. Four cultivation scenarios were designed: standard Earth conditions, chronic irradiation alone, simulated reduced gravity alone, and a combination of irradiation and reduced gravity. An analysis of the harvested microgreens revealed that growth was moderately reduced under chronic irradiation combined with altered gravity, likely due to oxidative stress, primarily concentrated in the roots. Indeed, an accumulation of reactive oxygen species (ROS) was observed, as well as of polyphenols, likely to counteract oxidative damage and preserve the integrity of essential structures, such as the root stele. These findings represent an important step toward understanding plant acclimation in space to achieve sustainable food production on orbital and planetary platforms.
Evaluating the Accumulation of Grain Mercury in Engineered Rice Lines Containing merA and merB Genes Under an Organic Mercury-Enriched Condition
Authors: Meng Deyao , Song Zhaoze , Liu Jinyuan , Ji Liyang , Chen Chen , Wang Changhu
Published: January 2025

Rice is a critical crop for human sustenance worldwide. Food security has increasingly attracted public concerns, particularly due to heavy metal pollution, which adversely impacts crop yield and quality, with cadmium and mercury being the primary culprits. Excessive soil mercury not only hampers rice’s growth and development but also leads to a substantial accumulation in grains, posing a significant threat to human health. To mitigate the issue, low-mercury germplasms in rice were developed by expressing bacterial merA and merB genes, which convert mercury to less toxic forms from its most hazardous organic form: methylmercury. While previous evaluations of transgenic lines were typically conducted in environments mimicking inorganic mercury enrichment, studies on their performance in organic mercury-rich conditions, such as year-round rice planting paddies, remain limited. In this study, merA and merB transgenic rice lines were cultivated in organic mercury-contaminated soil to evaluate their grain mercury accumulation. Results showed a reduction in total grain mercury contents across three transgenic lines. Notably, one combined merA and merB line exhibited decreased organic mercury accumulation, and a reduction in total mercury levels in its grains, highlighting its breeding potential as a low-mercury rice germplasm for breeding programs.
Broadband Normalized Difference Reflectance Indices and the Normalized Red–Green Index as a Measure of Drought in Wheat and Pea Plants
Authors: Sukhova Ekaterina , Zolin Yuriy , Popova Alyona , Grebneva Kseniya , Yudina Lyubov , Sukhov Vladimir
Published: January 2025

Global climatic changes increase areas that are influenced by drought. Remote sensing based on the spectral characteristics of reflected light is widely used to detect the action of stressors (including drought) in plants. The development of methods of improving remote sensing is an important applied task for plant cultivation. Particularly, this improvement can be based on the calculation of reflectance indices and revealing the optimal spectral bandwidths for this calculation. In the current work, we analyzed the sensitivity of broadband-normalized difference reflectance indices and RGB indices to the action of soil drought on pea and wheat plants. Analysis of the heat maps of significant changes in reflectance indices showed that increasing the spectral bandwidths did not decrease this significance in some cases. Particularly, the index RI(659, 553) based on the red and green bandwidths was strongly sensitive to drought action in plants. The normalized red–green index (NRGI), which was the RGB-analog of RI(659, 553) measured by a color camera, was also sensitive to drought. RI(659, 553) and NRGI were strongly related. The results showed that broadband and RGB indices can be used to detect drought action in plants.
Extracellular AMP Inhibits Pollen Tube Growth in Picea meyeri via Disrupted Calcium Gradient and Disorganized Microfilaments
Authors: Zhou Junhui , Yang Haobo , Zhang Yue , Cao Yuan , Jing Yanping
Published: January 2025

Adenosine monophosphate (AMP) is a hydrolysis product of adenosine triphosphate (ATP) and adenosine diphosphate (ADP). In mammalian cells, extracellular AMP functions as a signaling molecule by binding to adenosine A1 receptors, thereby activating various intracellular signaling pathways. However, the role of extracellular AMP in plant cells remains largely unclear, and homologs of A1 receptors have not been identified. Our previous studies have demonstrated that extracellular ATP (eATP) is crucial for the normal germination and growth of Picea meyeri pollen tubes. In the present study, we observed that the exogenous addition of ATP to a pollen culture medium could be degraded into AMP and adenosine. Furthermore, the addition of AMP and adenosine to the culture medium was found to inhibit pollen germination and tube elongation. Notably, the addition of an AMP receptor inhibitor into the culture medium mitigated the inhibitory effects of AMP on pollen tube growth. Through intracellular staining for Ca2+ and microfilaments, we discovered that high concentrations of AMP disrupt the Ca2+ concentration gradient and impair microfilament organization, ultimately resulting in inhibited pollen tube elongation. In conclusion, we propose that extracellular AMP, as a hydrolysis product of eATP, also plays a significant role in regulating P. meyeri pollen germination and tube growth in vitro.