bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 4 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Supplementary Table 4
Supplementary Figure 5 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Piperacetazine is not effective in preventing PAX3::FOXO1- mediated tumorigenesis at the tested dose.
Supplementary Figure 3 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Piperacetazine IC50 values across various cell lines.
FIGURE 4 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Piperacetazine alters the expression of PAX3::FOXO1 target genes. A, Protein expression of PAX3::FOXO1 target genes was evaluated by Western blot analysis in RH30 cells treated with 10 µmol/L piperacetazine for 6 days. B, RNA-seq was performed in fusion-positive RH30 cells treated with 30 µmol/L piperacetazine for 24 hours. The ranked gene expression list was compared with existing lists of genes using GSEA. Gene set descriptions: Top left: genes upregulated during human skeletal muscle myoblast differentiation. Top right: Hallmark genes during myogenesis. Bottom left: Genes downregulated in SAOS-2 (osteosarcoma) cells upon expression of PAX3::FOXO1. Bottom right: Genes downregulated in fusion-positive versus fusion-negative RMS cell lines. NES = normalized enrichment score, FDR = false discovery rate). C, Piperacetazine does not cause PAX3::FOXO1 to shift its intracellular localization. RH30 cells were treated with 10 µmol/L piperacetazine for 24 hours prior to cellular fractionation, which were analyzed via Western blot analysis. Lamin A/C and alpha-tubulin were used as positive controls for nuclear and cytoplasmic fractions, respectively. D, Piperacetazine does not alter PAX3::FOXO1 protein levels or phosphorylation of Ser256, as evaluated by Western blot analysis. RH30 cells were treated with 10 µmol/L piperacetazine or vehicle for 3 days. E, PAX3::FOXO1 protein was immobilized on a CM5 chip, and double-stranded PAX3::FOXO1 oligonucleotide (100 nmol/L), piperacetazine (10 µmol/L), or a combination of the two were injected over the chip surface. Piperacetazine did not inhibit DNA binding to PAX3::FOXO1.
FIGURE 3 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Piperacetazine inhibits endogenous PAX3::FOXO1 activity in multiple cell lines. A, The effect of piperacetazine treatment on cell viability was measured using the CellTiter-Blue assay (black lines). The effect of piperacetazine treatment on PAX3::FOXO1 activity was measured via luciferase assay (blue lines), using the PAX3::FOXO1-responsive pGL3-PDGFRA luciferase reporter (RH30, RH41, RH28, U66788, RD), and PGK reporter (SN12C, negative control). B, Left: Luciferase activity was measured in HEK293T cells transfected with PAX3::FOXO1-responsive ASS1P reporter and an empty or PAX3::FOXO1 expression vector. Cells were treated with DMSO or piperacetazine for 48 hours. Matching Western blots are shown under each bar. Right: The same experimental setup was repeated with EWS::FLI1-responsive NR0B1 luciferase reporter and an EWS::FLI1 expression vector or empty vector. The cells were treated with the same concentration of piperacetazine or DMSO for 48 hours, and the normalized luciferase readouts and accompanying Western blots are shown. (*, P < 0.0001; Student t test; ns, P > 0.05)
FIGURE 1 from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Purification of recombinant PAX3::FOXO1 protein from bacteria. A, PAX3::FOXO1 protein is expressed in E. coli. The most prominent bands following induction with isopropyl-beta-D-thiogalactopyranoside (IPTG) appeared just above the 100 kDa molecular weight marker on an 8% polyacrylamide gel stained with Coomassie blue. F17–F23 represent seven different fractions purified by column chromatography using the His tag on the carboxy terminal of the protein. B, The same purified fractions in A were used in a Western blot analysis to confirm protein identity using an anti-PAX3 antibody. C, DNA-binding specificity was evaluated by measuring binding of recombinant PAX3::FOXO1 and EWS::FLI1 proteins to double-stranded DNA oligonucleotides with binding sequences specific to either protein. PAX3::FOXO1 or EWS::FLI1 protein was immobilized on a CM5 chip and the indicated oligonucleotides were injected over the surface at a 1.25 to 80 nmol/L dose range. D, The quality of the PAX3::FOXO1 protein was evaluated by measuring its binding affinity to a double-stranded DNA oligonucleotide. PAX3::FOXO1 was immobilized on a CM5 chip and the oligonucleotide was injected over the surface at six concentrations (62.5, 12.5, 2.5, 0.5, 0.1, 0.02 nmol/L). Binding affinity was calculated by using a 1:1 binding model in BiaEvaluation software (χ2: 0.0424, U-value: 2). Colored lines indicate actual datapoints and black lines indicate curve fit used for affinity analysis.
Data from Piperacetazine Directly Binds to the PAX3::FOXO1 Fusion Protein and Inhibits Its Transcriptional Activity
Authors: Nakazawa Kay , Shaw Taryn , Song Young K. , Kouassi-Brou Marilyn , Molotkova Anna , Tiwari Purushottam B. , Chou Hsien-Chao , Wen Xinyu , Wei Jun S. , Deniz Emre , Toretsky Jeffrey A. , Keller Charles , Barr Frederic G. , Khan Javed , Üren Aykut
Published: October 2023

Abstract
The tumor-specific chromosomal translocation product, PAX3::FOXO1, is an aberrant fusion protein that plays a key role for oncogenesis in the alveolar subtype of rhabdomyosarcoma (RMS). PAX3::FOXO1 represents a validated molecular target for alveolar RMS and successful inhibition of its oncogenic activity is likely to have significant clinical applications. Even though several PAX3::FOXO1 function-based screening studies have been successfully completed, a directly binding small-molecule inhibitor of PAX3::FOXO1 has not been reported. Therefore, we screened small-molecule libraries to identify compounds that were capable of directly binding to PAX3::FOXO1 protein using surface plasmon resonance technology. Compounds that directly bound to PAX3::FOXO1 were further evaluated in secondary transcriptional activation assays. We discovered that piperacetazine can directly bind to PAX3::FOXO1 protein and inhibit fusion protein-derived transcription in multiple alveolar RMS cell lines. Piperacetazine inhibited anchorage-independent growth of fusion-positive alveolar RMS cells but not embryonal RMS cells. On the basis of our findings, piperacetazine is a molecular scaffold upon which derivatives could be developed as specific inhibitors of PAX3::FOXO1. These novel inhibitors could potentially be evaluated in future clinical trials for recurrent or metastatic alveolar RMS as novel targeted therapy options.
Significance:
RMS is a malignant soft-tissue tumor mainly affecting the pediatric population. A subgroup of RMS with worse prognosis harbors a unique chromosomal translocation creating an oncogenic fusion protein, PAX3::FOXO1. We identified piperacetazine as a direct inhibitor of PAX3::FOXO1, which may provide a scaffold for designing RMS-specific targeted therapy.
CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis
Authors: Zheng Jing , Chen Xi , Zhan Jia-Bin , Li Chang-Wu , Wei Xin , Jiang Hong-Yan
Published: October 2023

Introduction: Circular RNAs (circRNAs) are essential in the progression of allergic rhinitis (AR). The purpose of this research was to examine the role of circRNA ADP-ribosylation factor 3 (circARF3) in the pathogenesis of AR. Methods: To generate an animal model of AR, mice were treated with house dust mite (HDM), and mice nasal epithelial cells (NEpCs) were treated with IL-4/IL-13 to imitate the inflammatory damage of AR in vitro. Sanger sequencing, qRT-PCR, and RNAse R digestion assays all validated the circularization structure of circARF3. The levels of circARF3, miR-205-5p, and sirtuin 5 (SIRT5) were determined by qRT-PCR or Western blotting. Luciferase reporter, RNA immunoprecipitation, and pull-down experiments were used to investigate the regulatory network. Flow cytometry was used to investigate the rate of cell apoptosis, and Western blotting was used to determine the levels of apoptotic-related proteins (cleaved caspase 3, cleaved polyadenosine-diphosphate-ribose polymerase) and HMGB1, TLR4, and MyD88. Enzyme-linked immunosorbent assay was used to assess the inflammatory response. Hematoxylin-eosin staining and TUNEL were used to detect the histology of injury and apoptosis of nasal mucosa tissues. Results: CircARF3 and SIRT5 levels were reduced in HDM-treated animals and IL-4/IL-13-treated NEpCs, while miR-205-5p expression was increased. CircARF3 was generated by back-splicing exons 3–5 with a stable circular shape. CircARF3 overexpression mitigated IL-4/IL-13-induced apoptosis in NEpCs by inhibiting miR-205-5p. SIRT5 upregulation attenuated IL-4/IL-13-induced inflammatory injury in NEpCs, and SIRT5 knockdown induced opposite effects. miR-205-5p silencing reversed the effects of SIRT5 knockdown on IL-4/IL-13-induced inflammatory injury. Furthermore, circARF3 overexpression alleviated histological abnormalities, apoptosis, inflammatory response, and HMGB1/TLR4 signaling activation in HDM-treated animals. Conclusion: CircARF3 inhibited cell apoptosis and inflammation via the miR-205-5p/SIRT5 axis in IL-4/IL-13-treated NEpCs and HDM-treated mice.
A Scoping Review of mHealth Interventions for Secondary Prevention of Stroke: Implications for Policy and Practice
Authors: Allan Liam P. , Beilei Lin , Cameron Jan , Olaiya Muideen T. , Silvera-Tawil David , Adcock Amelia K. , English Coralie , Gall Seana L. , Cadilhac Dominique A.
Published: October 2023

Secondary prevention is a major priority for those living with stroke and may be improved through the use of mobile Health (mHealth) interventions. While evidence for the effectiveness of mHealth interventions for secondary prevention of stroke is growing, little attention has been given to the translation of these interventions into real-world use. In this review, we aimed to provide an update on the effectiveness of mHealth interventions for secondary prevention of stroke, and investigate their translation into real-world use. Four electronic databases and the gray literature were searched for randomized controlled trials of mHealth interventions for secondary prevention of stroke published between 2010 and 2023. Qualitative and mixed-methods evaluations of the trials were also included. Data were extracted regarding study design, population, mHealth technology involved, the intervention, and outcomes. Principal researchers from these trials were also contacted to obtain further translational information. From 1151 records, 13 randomized controlled trials and 4 evaluations were identified; sample sizes varied widely (median, 56; range, 24–4298). Short message service messages (9/13) and smartphone applications (6/13) were the main technologies used to deliver interventions. Primary outcomes of feasibility of the intervention were achieved in 4 trials, and primary outcomes of changes in risk factors, lifestyle behaviors, and adherence to medication improved in 6 trials. Only 1 trial had a hard end point (ie, stroke recurrence) as a primary outcome, and no significant differences were observed between groups. There was evidence for only 1 intervention being successfully translated into real-world use. Further evidence is required on the clinical effectiveness of mHealth interventions for preventing recurrent stroke, and the associated delivery costs and cost-effectiveness, before adoption into real-world settings.
Violence, Patronage Politics and Farm Ownership in Zimbabwean Land Reform
Authors: Zvokuomba Kwashirai , Batisai Kezia
Published: October 2023

This article explores the “violence–politics of patronage” nexus of land redistribution and ownership after land reform in Zimbabwe. Foregrounding an analysis of the violence on farms, which has been explored by many scholars in other dimensions, the article presents hard evidence of what happened on these farms post the Fast Track Land Reform Programme. Deploying Gramsci’s theory of hegemony, this article interrogates the sociocultural and political linkages of violence, patronage and farm ownership to illuminate the dark realities of land distribution after “jambanja” (farm invasions). The violent behaviour around resettled lands is a manifestation of a well-entrenched patronage system in which those whose political views diverge from the “hegemonic power” lose their land through unscrupulous acts. The violence of the jambanja era, initially targeted at white farmers within the context of a “nativist land revolution”, continues to manifest itself in communities, albeit differently. It is, as the French thinker, Jacques Mallet du Pan, said of the French Revolution, “the revolution devouring its own children”.
China Is in the Bible
Authors: Eichler Raanan
Published: October 2023

Isaiah 49:12 mentions “the land of Sinim.” Gesenius and most nineteenth-century scholars identified this place with China, but virtually all scholars today identify it instead with Aswan (Syene) in southern Egypt. It is argued here, based on the literary context, the wording “the land of [plural gentilic],” and the phonetics of Sinim, that the term means China.
Egypt: Recent Trends and Developments in Public Law
Authors: Abdou Mohamed
Published: October 2023

While there have been no major case-law developments in the sphere of public law, the reporting period was marked by legislative and policy efforts aimed at regulating new fields of technology. This trend is evidenced by the recent adoption of two bills on personal data protection and on the use of financial technology in non-banking financial activities. The contents of these bills are examined in further detail below.
Aortic disease 101: Breaking down myths of type A aortic dissection for patients and families
Authors: Martin Caroline D , Ruiz Michelle , Sorrell Anne , Sears Samuel F , McCarrick Gretchen , Bates Michael J , Degner Benjamin C
Published: October 2023

Objectives:

Patients with Type A aortic dissections are often overwhelmed by the influx of new information and changes necessary to adjust to life with an aortic disease. This article uses psychoeducation about life after dissection to dispel common myths about aortic disease and empower patients to live full lives. It is written in clear and easy-to-understand language to maximise patient comprehension and knowledge of their condition to help them feel more confident after Type A aortic dissection.

Design:

An interdisciplinary group of professionals (including cardiothoracic surgeons, a clinical cardiac psychologist, clinical cardiac psychology trainees, and genetic counsellors) were asked about their interest in creating an article written for patients who have undergone Type A aortic dissection repair. Those who agreed were then asked to report some of the myths commonly heard in practice about the procedure and its ramifications.

Setting:

The myths confronted in this article come from professionals working for a comprehensive heart care institute, the East Carolina Heart Institute in Greenville, NC, USA and Johns Hopkins Hospital in Baltimore, MD, USA.

Methods:

A multi-disciplinary group of professionals focused on aortic disease contributed myths commonly reported by patients seen in practice.

Results:

Common myths are presented and include safety fears about physical activity, diet, and recurrence of aortic dissection. By educating yourself about the facts of your diagnosis, you can feel as though you have more control over your health and your life.

Conclusion:

The takedown of common myths about life post-dissection can be helpful for patients with aortic disease and their families. Understanding the facts about a loved one’s new diagnosis can help partners and caregivers to be more fully involved in the patient’s care and feel confident. An important limitation of the present work is the lack of prior research on patient quality of life and mental health following Type A aortic dissection. Future work should explore the unique mental health needs of patients with aortic disease to create interventions that target mental health symptomatology to help patients live longer, healthier and richer lives.
Development and validation of the Cultural Responsiveness Assessment Measure (CRAM): A self-reflection tool for mental health practitioners when working with First Nations people
Authors: Smith Peter , Rice Kylie , Schutte Nicola , Usher Kim
Published: October 2023

Background:

The purpose of this study was to develop and to validate a measure of cultural responsiveness that would assist mental health practitioners across a range of disciplines, in Australia, to work with Indigenous clients.

Aim:

The Cultural Responsiveness Assessment Measure (CRAM) was developed to provide a tool for practitioners and students to evaluate their own culturally responsive practice and professional development.

Method:

Following expert review for face validity the psychometric properties of the measure were assessed quantitatively, from the responses of 400 mental health practitioners.

Results:

Confirmatory Factor Analysis yielded a nine factor, 36 item instrument that demonstrated strong convergent and discriminant validity as well as test-retest reliability.

Conclusions:

It is anticipated that the CRAM will have utility as both a learning tool and an assessment measure, for mental health practitioners to ensure that services are culturally responsive for Aboriginal and Torres Strait Islander people.
Characterization of nanocomposite tetrahedral amorphous carbon coated drill bits for improved drilled-hole quality in carbon fiber-reinforced polymer/Al7075-T6 stacks
Authors: Jebaratnam Joy Mathavan , Hassan Muhammad Hafiz , Mahmud Abdus Samad , Gérald Franz
Published: October 2023

The use of hybrid composite stacks is kept on increasing in the aircraft industry due to their numerous advantages in this sector. Therefore, improving the drilled-hole quality is also sought over time. The present work focuses on the effect of metallic elements-doped tetrahedral amorphous carbon coated drill bits on the drilled-hole quality while performing single-shot drilling of CFRP/Al7075-T6 stacks. This was evaluated from CFRP exit delamination and Al7075-T6 exit burr height. Mechanical and tribological properties of three different tetrahedral amorphous carbon (ta-C) coating-types, i.e. micro-, chromium-doped (:Cr) and titanium-doped (:Ti) were compared to uncoated tool ones. Bonding strength of the selected coatings with the tool was also calculated. Process capability six pack analysis was used to validate the experimental results obtained for drilled-hole quality. It was shown that micro-ta-C coating-type exhibited maximum hardness. The hardness value directly influences the exit delamination. It was found that CFRP exit delamination decreased as the hardness of the drill bit increased. Moreover, the multi-material stacks drilled with ta-C:Cr coated tool presented the lowest burr height (less than 150 µm), compared to the other tested coating-types. Low-friction surfaces seem to promote low exit burr height.
‘Doomed to Fail’: Dutch and West German Consulates Warning Against Mixed Marriages, 1950s–70s
Authors: de Hart Betty , Woesthoff Julia
Published: October 2023

This article demonstrates how official discourses in Western Europe warning against mixed marriages were built on colonial continuities as part of shared European heritage, as well as the importance of race and gender ideologies in those discourses. It addresses the exchange across borders of approaches to regulating ‘mixed’ marriages among Dutch and German consular officials, strongly advising European White women not to emigrate and not to marry Muslim men. Based on research in the archives of the Ministries of Foreign Affairs as well as newspaper archives in the Netherlands and West Germany, this article demonstrates the central role that consulates of both countries played in developing these official discourses. This study contributes to literature on female (e)migration as well as literature on present-day restrictive migration control practices by demonstrating the historical and colonial roots that still serve to justify state practices of regulating mixed intimacies in surveilling women's partner choice.
Omics-Based Mathematical Modeling Unveils Pathogenesis of Periodontitis in an Experimental Murine Model
Authors: Fujihara C. , Murakami K. , Magi S. , Motooka D. , Nantakeeratipat T. , Canela A. , Tanaka R. J. , Okada M. , Murakami S.
Published: October 2023

Periodontitis is a multifactorial disease that progresses via dynamic interaction between bacterial and host-derived genetic factors. The recent trend of omics analyses has discovered many periodontitis-related risk factors. However, how much the individual factor affects the pathogenesis of periodontitis is still unknown. This article aims to identify multiple key factors related to the pathogenesis of periodontitis and quantitatively predict the influence of each factor on alveolar bone resorption by omics analysis and mathematical modeling. First, we induced periodontitis in mice ( n = 3 or 4 at each time point) by tooth ligation. Next, we assessed alveolar bone resorption by micro–computed tomography, alterations in the gene expression by RNA sequencing, and the microbiome of the gingivae by 16S ribosomal RNA sequencing during disease pathogenesis. Omics data analysis identified key players (bacteria and molecules) involved in the pathogenesis of periodontitis. We then constructed a mathematical model of the pathogenesis of periodontitis by employing ordinary differential equations that described the dynamic regulatory interplay between the key players and predicted the alveolar bone integrity as output. Finally, we estimated the model parameters using our dynamic experimental data and validated the model prediction of influence on alveolar bone resorption by in vivo experiments. The model predictions and experimental results revealed that monocyte recruitment induced by bacteria-mediated Toll-like receptor activation was the principal reaction regulating alveolar bone resorption in a periodontitis condition. On the other hand, osteoblast-mediated osteoclast differentiation had less impact on bone integrity in a periodontitis condition.
External challenges for specialists dealing with forensic autopsies
Authors: Mohanty Manoj K , Sahu Sindhu S , Singh Sudipta R , Sahu Manas R , Swain Rajanikanta , Lenka Preetam K , Padhy Biswa M , Naveen Alagarasan
Published: October 2023

Background

In addition to poor infrastructure, and human resource constraints, forensic medicine specialists in developing countries face many external challenges.

Objectives

We aimed to investigate the prevalence, type, and source of external challenges confronting them including sex, age, religion, deceased's place of residence and the number of accompanying relatives.

Materials and methods

This cross-sectional, observational study was conducted between August 2020 and July 2022 at the Mortuary of AIIMS, Bhubaneswar with the approval of the ethical committee.

Results

Of note, forensic medicine specialists faced external challenges in about one in five cases (20.5%). Most demands were to either minimise the autopsy procedure (n = 65) or to conduct the autopsy at inappropriate times (n = 58). The demands to minimise the autopsy procedure were significantly associated with the deceased's age (p = 0.046), religion (p = 0.010), socioeconomic class (p = 0.020) and manner of death (p = 0.019).

Conclusions

Our study found that forensic medicine specialists in India face significant external challenges. Avoiding unnecessary complete autopsies, implementing night autopsies, and embracing minimally invasive autopsies are recommended to mitigate these challenges.
Fostering Gender Just and Emancipatory Campuses in HEIs: Interrogating the Role of Gender Cells and ICs
Authors: Pujari Leena
Published: October 2023

This paper is a reflexive essay that engages with the vexed question of gender equity on campuses of higher education. I argue that though our campuses are mired in complex hierarchies and inequalities, they are also sites of immense possibilities. These are spaces where critical conversations can emerge around gender and sexuality including an engagement with questions of caste, class, ableism so on and so forth. However, a transformative vision and an intersectional frame must inform these conversations. Two institutional mechanisms that can enable this radical re-imagination are Gender Cells and Internal Committees. However, this imagination has fallen far short of expectations. What might one possibly do to reinvigorate these mechanisms? Is there any way we can repose the trust in these bodies that were badly shaken following #metoo? Can we build alliances and forge solidarities with different constituencies on campus to work towards emancipatory campuses? This paper throws light on these and related issues.
The Pandemic, the Crisis of the Neoliberal Model, and the Emergence of Digital Capitalism: The New Labor Market Environment
Authors: Castillo Fernández Dídimo
Published: October 2023

The pandemic caused by the rapid spread of the SARS-CoV-2 coronavirus and the associated COVID-19 disease brought to light not only the foundations of Western modernity and its assumptions about economic and social development but also the huge contradictions of the prevailing globalizing model. The pandemic exposed the structural and intrinsic contradictions of neoliberal globalization. The current economic and social consequences are related to the deepening of that model and particularly to the history of the prevailing employment structures and social inequalities. The emerging scenarios are uncertain, but the acceleration of an already advanced process of digital capitalism appears in sight. This process will entail, as part of the “new normal,” new forms of precarity and social inequality.

La pandemia causada por la rápida propagación del coronavirus SARS-CoV-2 y la asociada enfermedad COVID-19 puso en evidencia no sólo los fundamentos de la modernidad occidental y sus supuestos sobre el desarrollo económico y social, sino también las enormes contradicciones del modelo globalizador imperante. La pandemia expuso las contradicciones estructurales e intrínsecas de la globalización neoliberal. Las consecuencias económicas y sociales actuales están relacionadas con la profundización de dicho modelo y, en particular, con la historia de las estructuras de empleo prevalecientes y las desigualdades sociales. Los escenarios emergentes son inciertos, pero la aceleración de un proceso ya avanzado de capitalismo digital es inminente. Este proceso implicará, como parte de la “nueva normalidad”, nuevas formas de precariedad y desigualdad social.
Case Reports of Low-Temperature Plasma Radiofrequency Treatment for Congenital Epiglottic Cysts in Neonates and Infants
Authors: Guo Shasha , Cao Changxing , Feng Chao , Li Zhengyang , Zhou Feng , Ye Huiping , Liang Qiulin
Published: October 2023

An uncommon benign condition in neonates, known as congenital epiglottic cyst, can lead to symptoms such as neonatal dyspnea, laryngeal stridor, and, in severe cases, even pose a potential threat of asphyxia-related mortality. A congenital epiglottic cyst is one of the neonatal emergencies. Fibrolaryngoscopy is the predominant method of diagnosis, and early identification, diagnosis, as well as treatment are the key points. Successful endoscopic ablation of congenital epiglottic cysts in 3 newborns was achieved through the utilization of a low-temperature plasma radiofrequency ablation system. Consequently, the purpose of this case report is to assist pediatric emergency physicians in the timely identification of congenital epiglottic cysts with the potential risk of mortality and to provide additional experience in clinical management.
Moral dilemmas in food provisioning: Inflation, the claim for ‘just prices’ and for ‘fair wages’
Authors: Narotzky Susana , Martínez Álvarez Bibiana
Published: October 2023

The cost of living we envision here stems from an interpretation of the ‘cost of living’ phrase which addresses (1) macro indicators of inflation, (2) the difference between farm gate price and consumer price as a cost to farmers that endangers their viability, and (3) how this cost transfers to the wages of workers and endangers their livelihood. Finally, (4) we wish to highlight that the energy that needs to be invested to assure social reproduction at the scale of individuals and households – workers and employers in agriculture – and at the scale of entire political communities such as the nation-state or the European Union, is translated into moral dilemmas that mediate and produce material results – in people’s bodies, in the environment, in political mobilizations of different kinds. The ‘cost of living’ here expands into the multiple and situated meanings of what it costs to live and the practices that they support.
The effect of low dose thyroid replacement therapy in patients with episodic migraine and subclinical hypothyroidism: A randomised placebo-controlled trial
Authors: Dev Priya , Favas T.T. , Jaiswal Rishab , Cyriac Mareena , Mishra Vijaya Nath , Pathak Abhishek
Published: October 2023

Introduction

Migraine is a common headache syndrome associated with various other comorbidities. Thyroid replacement in migraine patients with hypothyroidism improves headaches; however, thyroid hormone replacement in subclinical hypothyroidism is debatable, and its efficacy is not known.

Objective and methodology

This prospective, single-centre, quasi-randomised interventional study was conducted on patients visiting the General Medicine and Neurology outpatient department at a tertiary centre to look at the efficacy of thyroxine in subclinical hypothyroidism.

Results

We assessed 87 patients for analysis; no patients were lost to follow-up. There was a decrease in all parameters evaluated (headache frequency, severity, duration, MIDAS score, MIDAS grade) at three months of follow-up in the treatment group compared to placebo group. There was a significant decrease in headache frequency and severity in the levothyroxine group compared to the placebo group at three months of follow-up. Also, the follow-up MIDAS score (mean ± SD: 6.30 ± 2.455 scores vs. 8.45 ± 5.757 scores) was significantly decreased by treatment at three months follow-up.

Conclusion

Treatment of subclinical hypothyroidism effectively reduces migraine headaches, and it is logical to check thyroid function status in patients presenting with migraine headaches. However, a larger randomised controlled trial is required to prove the efficacy of levothyroxine in migraine with subclinical hypothyroidism.
Determinants of Usage and Satisfaction with Demand Responsive Transport Systems in Rural Areas
Authors: Gkavra Roxani , Susilo Yusak Octavius , Klementschitz Roman
Published: October 2023

Mobility in rural areas requires increased attention, because of not only the large share of the population living there but also the difficulties and special needs that characterize the areas themselves and their residents and visitors. Demand responsive transport (DRT) systems can fill the gap for flexible and sustainable public transport in rural environments. However, since most past studies focused on the urban context, there is still a lack of knowledge on the determinants of usage and loyalty to these systems. The objective of the present study is to identify the decisive factors of DRT systems’ usage in rural areas by examining user characteristics, usage cases, and the determinants of satisfaction with such services. Insights are obtained based on survey data collected on two DRT systems in Austria—one system with a flexible timetable and predetermined stops that mainly serves suburban residents and another door-to-door service with a fixed timetable that is oriented to locals and tourists. The usage of DRT systems is modeled via regression models and the decisive factors are identified and compared among user groups and services. The findings suggest that DRT systems are used for both compulsory and non-mandatory trips and that the majority of users are female. However, the results from different user groups and services suggest that there are not unanimous determinants of the probability of traveling by DRT. With regard to overall satisfaction, it is found that it is related to satisfaction with both trip-level attributes and system design characteristics.
Uniaxial Compressive Process Simulation of Geocell-Reinforced Asphalt Mixture by Discrete Element
Authors: Xianrong Wang , Shangyu Han , Yunsheng Zhu
Published: October 2023

Based on laboratory tests and the discrete element method (DEM), this study investigated the reinforcement by geocells on the compressive process of asphalt mixture. By considering the vital material features and mechanical properties of different ingredients (the three-dimensional irregular shapes and elastic properties of coarse aggregates and geocells, the viscoelastic properties of asphalt mastic, and the random distribution of air voids within asphalt mastic), micromechanical modeling of geocell-reinforced asphalt mixture and virtual uniaxial compression test was built by using PFC3D. The effects of geocell reinforcement on the compression process of the asphalt mixture were evaluated through the virtual compression test. The results show that the built DEM and virtual test can effectively predict the compression process of geocell-reinforced asphalt mixture. The geocell reinforcement positively affected the compression process of asphalt mixture by affecting the distribution of micromechanical forces and displacements in the asphalt mixture. Moreover, the geocell reinforcement was more significant on asphalt mastic. This indicates that the geocell significantly improves the compressive performance of asphalt mixture from the microscopic perspective.
Mechanical and tribological performances of ceramic microsphere reinforced polyamide 6 composites
Authors: Savas Soner , Pecenek Hilal , Atabek Savaş Lemiye , Dogan Mehmet
Published: October 2023

The main motivation of this work is to use ceramic microspheres (CMs) as a potential filler in polyamide 6 (PA6) to enhance its mechanical and tribological performances. Polyamide 6 composites bearing CMs in different weight ratios (10%–40%) are processed by melt blending and characterized primarily by tensile and bending test, dynamical mechanical analysis, and ball-on-disc wear test. This work clearly shows that the addition of CMs gives rise to superior mechanical and wear resistance contribution to PA6. The tensile strength, tensile modulus, flexural strength, and flexural modulus of the composites are enhanced up to 11%, 25%, 37%, and 80%, respectively, over those of the pristine polymer. The wear resistance of 40 wt% CMs containing composite is 60% higher than that of pure PA6. Consequently, it has been found that CMs can be a suitable alternative especially in applications where mechanical strength is desired due to its advantages of high rigidity and sliding wear resistance.
Improving Older Adults’ Functional Health Using the Progressive Stay Strong, Stay Healthy Program
Authors: NJ Spokely , KJ Weitzel , KA Miller , Oliveira M Novaes , SD Ball , BS Baker
Published: October 2023

This study assessed the Stay Strong, Stay Healthy (SSSH) level two resistance training program in 406 older adults (69.3 ± 8.1 years). SSSH included 10 exercises completed during 60 minute sessions, which were offered twice weekly for eight weeks. 30-second sit-to-stand (30STS), timed-up-and-go (TUG), flexibility, and balance poses were completed pre/post-SSSH. Paired t-tests with Bonferroni corrections showed the whole group improved 30STS, TUG, flexibility, and total balance scores over time ( p < .001). Repeated measures analyses of variance showed both geography and age groups improved 30STS, TUG, flexibility, and total balance scores over time (all time effects p ≤ .039). Independent t-tests showed rural participants improved to a similar or greater absolute extent than their urban neighbors for nearly all measures and all age groups improved to a similar absolute magnitude over time. This study demonstrates SSSH level two improves physical function in older adults and these benefits are similar across geography and age groups.
Impacts of the COVID-19 Response on the Domestic Violence Workforce
Authors: Wells Sarah A. , Fleury-Steiner Ruth E. , Miller Susan L. , Camphausen Lauren C. , Horney Jennifer A.
Published: October 2023

Many frontline and essential workers faced increased levels of stress, anxiety, depression, and even suicide ideation during the pandemic response. These and other factors led to burnout, shifts into non-patient or client-facing roles, or leaving an occupation altogether. Domestic violence advocates experienced increases in many types of stressors as they continued to provide essential services to victims and survivors during the pandemic. However, in most cases they did so without protections offered to essential workers, like priority access to personal protective equipment (PPE) or vaccines. Executive directors of U.S. State and Territorial Domestic Violence Coalitions were identified using the National Network to End Domestic Violence website and contacted via email to schedule key informant interviews. Interviews were conducted, recorded, and transcribed using Zoom. Themes were identified using both inductive and deductive coding. Twenty-five of 56 (45%) coalition executive directors completed an interview. Three main themes related to workforce were identified, including an accelerated rate of job turnover among both leadership and staff; a lack of essential worker status for domestic violence advocates; and unsustainable levels of stress, fear, and exhaustion. While familiar challenges drove these outcomes for this predominantly female, low-wage workforce, such as a lack of access to childcare, other factors, including the lack of access to PPE, training, and hazard pay for those working in person, highlighted inequities facing the domestic violence workforce. The factors identified as impacting the domestic violence workforce—turnover, low status, and high levels of stress, fear, and exhaustion—made the already challenging provision of advocacy and services more difficult. Domestic violence advocates are essential first responders and must be supported in ways that increase the resilience of empowerment-based services for victims and survivors.
Purge the Evil From Your Midst: Material Cancel Culture Among Religiously Observant Jews
Authors: Kravel-Tovi Michal , Malev Tzofiya , Nisan Schwarzfuchs Eran
Published: October 2023

This article explores cancel culture as a material endeavor on the ground. We draw on ethnographic tools to call attention to formative material dimensions of canceling and employ the conceptual lens of material culture to analyze rationales and practices around the canceling of objects. The case study concerns how ultra-Orthodox Jews in Israel manage allegations of sexual abuse against a beloved author by engaging in a myriad of material-canceling actions around his books. We cluster and investigate these actions under three categories: Time-Out, Taking Out, and Casting Out. We offer the term Material Cancel Culture as a productive intervention in a literature that generally focuses on the discursive and digital components of cancel culture. The multiplicity of possible material (dis)engagements allows us to move beyond a somewhat dichotomous outline of cancel versus not-cancel, and to consider how the material immediacy and properties of objects open up new scripts of action in response to public sentiments of rage and critique.
Application of Virginia Henderson’s Theory Within an Intimate Partner Violence Scenario
Authors: Abdul Majeed Ina , Jivani Kiran Karim , Mughal Farida Bibi , Karim Kanwal , Mistri Naureen , Shamsher Ali Sabeen , Kabir Abdul , Carroll Karen
Published: October 2023

The practice column focuses on incorporating a nursing theoretical framework into nursing care in a clinical scenario involving intimate partner violence. Accordingly, as exemplified by utilizing Virginia Henderson’s theory, there is guidance to define, focus, and evaluate nursing care in these multifaceted clinical cases.
Paradoxes and Patterns in Nursing Practice
Authors: Carroll Karen
Published: October 2023

In the article that follows the authors present information focused on nursing care delivery involving intimate partner violence and how to structure, evaluate, and improve nursing care. The authors chose the structure of Virginia Henderson’s nature of nursing to evaluate nursing care and review the paradoxes and patterns lived in a journey of improving nursing care. This brings to light the importance of paradoxes and patterns of nursing care and the importance of utilizing a nursing theory-based approach.
Poetry: An Avenue of Artsciencing
Authors: Bunkers Sandra Schmidt
Published: October 2023

As an introduction to the inaugural Artistic Expression Column, the construct of artsciencing is briefly discussed and poetry is presented as an avenue of artsciencing.
A Dialogue with Dr. Marilyn A. Ray: Nurse Scholar and USAF Veteran
Authors: Morrow Mary R. , Ray Marilyn A.
Published: October 2023

Dr. Marilyn A. Ray, nurse scholar and retired United States Air Force (USAF) veteran and former flight nurse, began her nursing scholarship in Canada. She was influenced by the experiences and interprofessional scholarly ideas that she encountered along her career trajectory. Her early love of the air and space led her to the United States Air Force Nurse Corps, where she served as a flight nurse during the Vietnam war era, followed by leadership positions in nursing education, administration, practice, and research. Dr. Ray’s contributions to nursing knowledge includes two nursing theories and a caring inquiry methodology. Dr. Ray is helping to create a new caring science certificate program at Florida Atlantic University, Christine E. Lynn College of Nursing. In this column, Dr. Ray shares the story of her scholarly influences and how they helped her care for her husband and gain insight into her contributions to nursing knowledge development.
Adaptive fuzzy command–filtered control strategy for electro-hydraulic braking systems of winding hoist with prescribed performance
Authors: Xie Hui , Shen Gang , Tang Yu , Li Xiang
Published: October 2023

In the braking control of winding hoisting systems, proper braking strategy is of great significance in reducing the probability of significant hidden dangers and improving the safety and reliability of the system. However, as a typical elastic and variable stiffness system, the winding hoisting systems have large nonlinearity, parameter uncertainties, random disturbances, and so on, which severely restrict the improvement of braking performance. To this end, an adaptive fuzzy command–filtered controller with prescribed performance is developed for electro-hydraulic braking systems of winding hoist in this article. First, the dynamic model of the winding hoisting system is established with consideration of the elasticity of wire rope. Based on the established dynamic model, using the fuzzy logic system, the algebraic loop problem caused by non-strict feed structure is overcome, and the unknown nonlinearities arising from modeling error, parameter uncertainties, and system disturbances are also approximated for the subsequent active unknown nonlinearities compensation. Next, to enhance the convergence rate and guarantee the position tracking error within a prescribed region, a tracking error transformation method based on the prescribed performance function is utilized. Subsequently, the adaptive fuzzy control algorithm is developed with command filters, which can also avoid the complexity explosion issue due to the analytic calculation of the derivatives of stabilizing functions, and the rigorous stability of the close-loop system is proved by the Lyapunov theory. Finally, experimental results are utilized to illustrate the effectiveness of the developed controller.
Rapid laminated clastic alluviation associated with increased Little Ice Age flooding co-driven by climate variability and historic land-use in the middle Severn catchment, UK
Authors: Pears Ben , Brown Antony G , Toms Phillip S , Wood Jamie , Pennington Benjamin T , Jones Richard
Published: October 2023

The analysis of exceptionally well-preserved visible clastic laminations in deep alluvial sediments at Kempsey, Worcestershire (UK), allows a new high-resolution analysis of late-Holocene flood-history in the largest UK catchment, as well as local human response. At the sample site over 4.5 m of sandy-silt overbank-alluvium accumulated on the floodplain and optically stimulated luminescence (OSL) dating of the upper 2.25 m demonstrates accretion from the late 14th century CE onwards. Sub-centimetre to centimetre resolution multi-proxy sediment analysis (loss on ignition, magnetic susceptibility, particle size, ITRAX and portable XRF) demonstrate clear variations in depositional history over the last millennium due to channel stability. Between c. 1380 and 1550 CE overbank sedimentation was driven by lower energy flood events, with negligible effect from climatic conditions during the Spörer Minimum (1460–1550 CE). After c. 1550 CE the magnitude of flooding events increased and by c. 1610 CE, the start of the visible sub-centimetre laminations, the accumulation rate regularly exceeded 3 mm year−1, which increased to 4.5 mm year−1 between c. 1690 and 1710 CE, and 3 and 3.5 mm year−1 between c. 1790 and 1840 CE before alluviation was altered by an embankment. The greatest extent of coarse overbank deposition and increased accumulation rate occur concurrently with periods of climatic instability associated with the Maunder (1645–1715 CE) and Dalton (1790–1820 CE) Minima, the periods of largest historical floods and during the intensification of arable cultivation across the middle Severn catchment. This data correlates well with other sites in the catchment suggesting that these are basin-wide forcing-responses. We also present evidence that this catchment-wide hydro-geomorphological history had local effects in shifting the geographical focus of an important settlement away from its historic floodplain edge location – which can be viewed as an adaptation to the flood risk.