bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantum Automated Tools for Finding Impossible Differentials
Authors: Xie Huiqin , Xia Qiqing , Wang Ke , Li Yanjun , Yang Li
Published: August 2024

Due to the superiority of quantum computing, traditional cryptography is facing a severe threat. This makes the security evaluation of cryptographic systems in quantum attack models both significant and urgent. For symmetric ciphers, the security analysis heavily relies on cryptanalysis tools. Thus, exploring the use of quantum algorithms in traditional cryptanalysis tools has garnered considerable attention. In this study, we utilize quantum algorithms to improve impossible differential attacks and design two quantum automated tools to search for impossible differentials. The proposed quantum algorithms exploit the idea of miss-in-the-middle and the properties of truncated differentials. We rigorously prove their validity and calculate the quantum resources required for their implementation. Compared to the existing classical automated cryptanalysis, the proposed quantum tools have the advantage of accurately characterizing S-boxes while only requiring polynomial complexity, and can take into consideration the impact of the key schedules in a single-key model.
Marine Products and Their Anti-Inflammatory Potential: Latest Updates
Authors: Vasarri Marzia , Degl’Innocenti Donatella
Published: August 2024

The depths of the sea are a rich source of biologically active compounds with therapeutic potential for various human diseases, including inflammatory conditions [...]
The Effect of Intercropping with Different Leguminous Green Manures on the Soil Environment and Tea Quality in Tea Plantations
Authors: Zhou Pinqian , Chen Mengjuan , Bao Qiang , Wang Hua , Wang Yuanjiang , Fu Haiping
Published: August 2024

Intercropping with green manure is a soil-sustainable cultivation practice that has demonstrated positive impacts on tea growth and the soil environment in tea plantations. Nevertheless, research examining the effect of leguminous green manure varieties in tea plantations is scarce. This study aimed to analyze the tea quality and soil environment components in response to intercropping with three distinct leguminous green manures, Cassia sophera cv. Chafei 1 (CF), Sesbania cannabina (Retz.) Pers. (SC), and Chamaecrista rotundifolia (Pers.) Greene (CR), with 70% chemical fertilizer, and compare them to non-intercropped green manures with 100% chemical fertilizer (CK) in tea plantations. The findings indicated that intercropping with SC increased the amino acids content of tea leaves, the soil organic carbon (SOC), the soil acid phosphatase (ACP), the soil acid protease (ACPT), and the bacterial diversity compared to the CK treatment. Intercropping with CR improved the ACP activity and bacterial diversity while intercropping with CF improved the polyphenols. Proteobacteria, Acidobacteria, Actinomycetes, and Firmicutes were identified as the dominant bacterial taxa in tea plantations with intercropped green manure. A strong positive correlation was indicated between the SOC contents and the amino acids content in tea leaves after intercropping. A canonical correspondence analysis indicated significant associations between the ACP and the urease activity, and between the ACP and ACPT, and both were closely linked to SC. This finding provides an explanation that intercropping with SC may positively affect tea quality by influencing the SOC content, the soil enzyme activity, and the soil bacterial diversity. Green manure intercropping may replace part of chemical fertilizers, improve the soil environment in tea gardens, and enhance the quality of tea. These findings offer a theoretical reference for selecting leguminous green manure and advancing the sustainable development of tea plantations.
Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis
Authors: Sołowińska Karolina , Holec-Gąsior Lucyna
Published: August 2024

Toxoplasmosis represents a significant public health and veterinary concern due to its widespread distribution, zoonotic transmission, and potential for severe health impacts in susceptible individuals and animal populations. The ability to design and produce recombinant proteins with precise antigenic properties is fundamental, as they serve as tools for accurate disease detection and effective immunization strategies, contributing to improved healthcare outcomes and disease control. Most commonly, a prokaryotic expression system is employed for the production of both single antigens and multi-epitope chimeric proteins; however, the cloning strategies, bacterial strain, vector, and expression conditions vary. Moreover, literature reports show the use of alternative microbial systems such as yeast or Leishmania tarentolae. This review provides an overview of the methods and strategies employed for the production of recombinant Toxoplasma gondii antigenic proteins for the serological detection of T. gondii infection and vaccine development.
Distribution and Characterization of Quaternary Ammonium Biocides Resistant Bacteria in Different Soils, in South-Western China
Authors: Guo Ziyi , Qin Cunli , Zhang Lilan
Published: August 2024

Quaternary ammonium compounds (QACs) are active ingredients in hundreds of disinfectants for controlling the epidemic of infectious diseases like SARS-CoV-2 (COVID-19), and are also widely used in shale gas exploitation. The occurrence of QAC-resistant bacteria in the environment could enlarge the risk of sterilization failure, which is not fully understood. In this study, QAC-resistant bacteria were enumerated and characterized in 25 soils collected from shale gas exploitation areas. Total counts of QAC-resistant bacteria ranged from 6.81 × 103 to 4.48 × 105 cfu/g, accounting for 1.59% to 29.13% of the total bacteria. In total, 29 strains were further purified and identified as Lysinibacillus, Bacillus, and Klebsiella genus. There, bacteria covering many pathogenic bacteria showed different QACs tolerance with MIC (minimum inhibition concentration) varying from 4 mg/L to 64 mg/L and almost 58.6% of isolates have not previously been found to tolerate QACs. Meanwhile, the QAC-resistant strains in the produced water of shale gas were also identified. Phylogenetic trees showed that the resistant species in soil and produced water are distinctly different. That is the first time the distribution and characterization of QAC-resistant bacteria in the soil environment has been analyzed.
“Yet Once More”: John Milton’s Lycidas as an Assault on the Ordinary
Authors: Clemens Justin
Published: August 2024

This article examines a problematic of the ordinary as it emerges in the poetical theology of an early poem of John Milton. This poem, Lycidas, has captured the attention of every major critic from the 18th century to the present, who has minutely examined its odd formal and generic character, its peculiar mix of personal grief and political outrage, and its role in Milton’s own personal development at a particularly decisive moment in English history. Yet, despite this extensive interpretive history, ‘the ordinary’ has never become an extended object of critical analysis. This article accordingly seeks to uncover and examine the importance of a certain set of contemporaneous significations of the ordinary in Lycidas, which implicate the institutions of Church, state, and university in perhaps surprising ways.
Selection and Optimization of China’s Energy Transformation Pathway Under Carbon-Neutral Targets
Authors: Qi Yingying , Yu Guohua
Published: August 2024

This paper uses a bottom-up national energy technology model to study the optimization of China’s energy transformation pathway. The model clarifies specific action plans for China’s energy transformation pathway from 2020 to 2060, total carbon emissions, industry emission reduction responsibilities, and other dimensions. The results show that: (1) The proportion of renewable energy consumption in China’s entire energy system from 2020 to 2060 will gradually exceed that of fossil energy under ideal circumstances, and the energy system will transition from traditional fossil energy to renewable energy. Meanwhile, the proportion of low-carbon energy sources, such as renewable energy, in primary energy demand will jump from 15.9% in 2020 to over 80% by 2060. (2) China’s CO2 emissions will be approximately 3 billion tons, 2 billion tons, and 1 billion tons under three different socio-economic development scenarios of low, medium, and high speed in 2060. At that time, China will still need to absorb CO2 through carbon sinks in forests, oceans, and wetlands. (3) The electricity industry has the highest CO2 emissions compared to other industries. The electricity industry must bear significant responsibility for carbon reduction in future energy transformation and economic development.
Optimizing Continuous Casting through Cyber–Physical System
Authors: Regulski Krzysztof , Rauch Łukasz , Hajder Piotr , Bzowski Krzysztof , Opaliński Andrzej , Pernach Monika , Hallo Filip , Piwowarczyk Michał , Kalinowski Sebastian
Published: August 2024

This manuscript presents a model of a system implementing individual stages of production for long steel products resulting from rolling. The system encompasses the order registration stage, followed by production planning based on information about the billet inventory status, then offers the possibility of scheduling orders for the melt shop in the form of melt sequences, manages technological knowledge regarding the principles of sequencing, and utilizes machine learning and optimization methods in melt sequencing. Subsequently, production according to the implemented plan is monitored using IoT and vision tracking systems for ladle tracking. During monitoring, predictions of energy demand and energy consumption in LMS processes are made concurrently, as well as predictions of metal overheating at the CST station. The system includes production optimization at two levels: optimization of the heat sequence and at the production level through the prediction of heating time. Optimization models and machine learning tools, including mainly neural networks, are utilized. The system described includes key components: optimization models for sequencing heats using Ant Colony Optimization (ACO) algorithms and neural network-based prediction models for power-on time. The manuscript mainly focuses on process modeling issues rather than implementation or deployment details. Machine learning models have significantly improved process efficiency and quality; the optimization of planning has reduced sequencing plan execution time; and power-on time prediction models estimate the main ladle heating time with 97% precision, enabling precise production control and reducing overheating. The system serves as an example of implementing the concept of a cyber–physical system.
Integrating MILP, Discrete-Event Simulation, and Data-Driven Models for Distributed Flow Shop Scheduling Using Benders Cuts
Authors: Wallrath Roderich , Franke Meik B.
Published: August 2024

Digitalization plays a crucial role in improving the performance of chemical companies. In this context, different modeling, simulation, and optimization techniques such as MILP, discrete-event simulation (DES), and data-driven (DD) models are being used. Due to their heterogeneity, these techniques must be executed individually, and holistic optimization is manual and time-consuming. We propose Benders decomposition to combine these techniques into one rigorous optimization procedure. The main idea is that heterogeneous models can simultaneously be optimized as Benders subproblems. We illustrate this concept with the distributed permutation flow shop scheduling problem (DPFSP) and assume that a MILP, DES, and DD model exist for three flow shops. Our approach can compute bounds and report gap information on the optimal makespan for five medium-sized literature instances. The approach is promising because it enables the optimization of heterogeneous models and makes it possible to build optimization capabilities on an existing model and tool landscape in chemical companies.
Study on the Evolution of Mechanical Properties and Acoustic Emission of Medium-Permeability Sandstone under Multi-Level Cyclic Loading Stress Paths
Authors: Xia Debin , Liu Hejuan , Liu Jianjun , Guo Yintong , Liu Mancang , Qiu Xiaosong , Li Haibo , Tan Hongying , Lu Jun
Published: August 2024

Depleted gas reservoirs are important natural gas storage media, thus research on the mechanical properties and damage evolution of reservoir rocks under alternating load conditions has significant practical implications for seal integrity studies. This paper conducted multi-level cyclic loading triaxial compression experiments on medium-porosity medium-permeability sandstone under different confining pressures and used acoustic emission (AE) instruments to detect the AE characteristics during the experiment, analyzing the mechanical characteristics, AE, and damage evolution characteristics. The experimental results show that after cyclic loading, the peak strength of sandstone increased by 14–17%. With the increase in the upper limit stress of cyclic loading, the elastic modulus showed a trend of first increasing and then gradually decreasing. The damage variable of rock samples rose with a rise in the upper limit stress of cyclic loading and confining pressure, and the rock damage was mostly localized at the peak stress. The AE b-value increased generally as confining pressure increased, showing that fractures occurred quicker and more unevenly at lower confining pressures. The distribution of RA-AF values shows that a sudden increase in stress causes the initiation and expansion of cracks in medium-permeability sandstone, and that tensile and shear cracks form continuously during the cyclic loading process, with shear cracks developing more pronounced. This research can provide some theoretical guidance for the long-term stable operation and pressure enhancement expansion of depleted gas reservoir storage facilities.
Pressurized Regenerative Calcium Cycle for Utility-Scale Energy Storage: A Techno-Economic Assessment
Authors: Moghtaderi Behdad , Tremain Priscilla , Warner John
Published: August 2024

The University of Newcastle (UON) and Jord International Pty Ltd. (Jord) have jointly developed a novel concept for the storage of energy from renewable and fossil fuel sources. The process, referred to as the pressurized regenerative calcium cycle (PRC2), relies on cyclic carbonation and calcination of CaO/CaCO3, in which low-cost electrical energy (i.e., off-peak, or excess generation from renewables) drives the calcination reaction and electricity is generated as required through the carbonation reaction. Initial proof-of-concept testing of the process was previously conducted within an existing fluid bed reactor at UON. The PRC2 concept was successfully demonstrated by maintaining the fluid bed reactor at a constant temperature by using the heat released during the reaction of calcium oxide and carbon dioxide. Following proof-of-concept testing, further refinement of the PRC2 process, which is the subject of this paper, was conducted to address its shortcomings and, importantly, facilitate the detailed design, construction, and operation of a large-scale demonstration plant. Nine different configurations were examined for the PRC2 process, for each of which a combined experimental, process modelling, and techno-economic assessment was completed. Experimental investigations were conducted to determine the suitability of carbonate materials for the PRC2 process. Process modelling and levelized cost of storage (LCOS) calculations were concurrently conducted and revealed that the molten salt approach (Option 9) was the most promising, having superior round-trip efficiency and lowest LCOS. For practical reasons (e.g., technical difficulties of working with molten salts), Option 3 (indirect power generation using a fluid bed reactor) was deemed the most feasible option for a demonstration scale plant. The LCOS for Option 3 (assuming a 100 MWe capacity) was calculated to be AUD 245 per MWh, which is on par with the cost of batteries for peak power replacement applications (the cost associated with lithium-ion batteries is AUD 370 per MWh).
Gender in Portuguese Firefighters: The Experiences and Strategies of Women
Authors: Lopes Beatriz Gomes , Marques António Manuel , Santos Maria Helena
Published: August 2024

This study sets out to analyze the negative effects of tokenism among a group of Portuguese female firefighters alongside the strategies they adopt to integrate into a male majority professional context and inherently associated with masculinity. Individual semi-structured interviews were carried out with nine men and nine women before their content was subject to thematic analysis. The results confirm how, in the context of the work performed by firefighters, there is a clear differentiation between the genders, with women both being more visible and leading to conformity to depicting stereotypical femininity. In order to fit in, female firefighters attempt to replicate the attitudes of their male colleagues, demonstrate high standards of performance, and tone down any expression of perceivably feminine characteristics.
From Nuclear to Diverse: Shifting Conceptualisations of Marriage among Australia’s 1960s Generation—A Qualitative Study
Authors: Phillips Matthew James
Published: August 2024

In contemporary times, the conceptualisation of marriage has shifted; however, societal attitudes and cultural ideologies regarding its construction remain complex. As such, unique generational perspectives are needed to reflect on how marriage has evolved and to comment on ongoing tensions related to it. This study explores how individuals born in the 1960s conceptualise marriage, examining the complex interplay between traditional values and evolving perspectives. Using an exploratory qualitative design underpinned by a social constructionist epistemology, semi-structured interviews were conducted with 12 participants (7 female, 5 male) aged 56–65. Reflexive thematic analysis revealed three main themes: (1) Unveiling the New Vows: The Shifting Values of Marriage and Family, (2) Breaking Boundaries and Transforming Traditions: Debunking Heteronormativity, and (3) Witnessing Progression: Dismantling Barriers and Embracing Diversity and Equality in Marriage. The findings indicate a significant shift in how marriage is viewed, with participants demonstrating both nostalgia for traditional constructs and support for more inclusive definitions. Notably, many participants positioned themselves as more progressive than their peers, challenging notions of homogenous generational attitudes. This study highlights the ongoing tension between traditional and contemporary views of marriage, suggesting that efforts to promote inclusivity must address both legal barriers and deeply ingrained cultural norms. These findings contribute to our understanding of how social institutions like marriage are reconceptualised over time, emphasising the dynamic nature of social change and the capacity for individuals to evolve in their perspectives on deeply entrenched societal norms.
Intensity and Pace Calculation of Ultra Short Race Pace Training (USRPT) in Swimming—Take-Home Messages and Statements for Swimming Coaches
Authors: Papadimitriou Konstantinos
Published: August 2024

A recently referenced method known as ultra short race pace training (USRPT), designed to familiarize swimmers with the pace of a swimming event by using high volumes and submaximal intensities, has emerged as an efficient approach, enhancing performance and predicting swimming outcomes. Despite its recognized benefits, particularly its lower physiological burden compared to other training methods, research on USRPT is still in its early stages. There are misunderstandings related to its intensity and the pace of calculation. This systematic review aims to provide valid statements identifying the pros and cons of USRPT as a training stimulus and providing swimming coaches with key messages and advice about this training method. For the analysis, 90,612 studies from PubMed, EBSCO, Science Direct, and Google Scholar databases were screened to research the background, intensity, and pace calculation of the USRPT method, although only four met the inclusion criteria. The final screening of the selected studies was conducted using a PRISMA-P document. USRPT has the potential to become a dominant training stimulus, offering a precise alternative to the often vague training sets that many swimmers use. However, further studies focusing on specific aspects of intensity and pace calculation within USRPT sets are needed for comprehensive understanding. In conclusion, USRPT appears to be a submaximal variation of high-intensity interval training (HIIT) with low blood lactate relevance to swimming events. Also, the pace calculation must be implemented considering the different demands of each point of a swimming event.
Liver Biopsy Technique for Analysis of Hepatic Content during Pregnancy and Early Lactation in Dairy Goats
Authors: de Oliveira Aline Marangon , de Faria Anna Luiza Silva , Quirino Daiana Francisca , Schultz Érica Beatriz , Rennó Luciana Navajas , Rodrigues Marcelo Teixeira , Veloso Cristina Mattos
Published: August 2024

Biopsy techniques in dairy goats are currently limited. This study aimed to describe a liver biopsy technique in dairy goats and to evaluate liver triglyceride levels and glycogen content. Sixty-nine dairy goats in the final stage of pregnancy and early lactation period were selected. Fifty goats were selected randomly for hepatic biopsy (HB) according to gestational period and were characterized according to fetus number (single: n = 16, multiple: n = 34), supplementation with propylene glycol (diet: n = 23, diet+PG: n = 27), and milk production levels (high: 3.0 ± 0.4 L/day, n = 15; low: 1.4 ± 0.4 L/day, n = 26). Liver tissue samples were obtained through biopsy on days −30, −20, −15, −10, −5, and 15 days after calving. Hepatic triglyceride and glycogen were quantified. The results were analyzed using the F-test at a 5% significance level and a comparison of means using the Tukey test. The liver biopsies did not influence dry matter intake, body weight, or milk yield. Hepatic glycogen concentration was lower 15 days after calving than it was prior to calving, except on day −20. Goats that generated high levels of milk production had lower triglyceride levels than goats that generated low levels of milk production. The biopsy technique is a safe method for obtaining tissue and evaluating liver content in dairy goats. The milk production level and days relative to parturition influence the hepatic triglyceride and glycogen content in dairy goats.
Gambaran Tingkat Pengetahuan Tentang Kesehatan Gigi Dan Mulut Sebelum Dan Sesudah Penyuluhan Menggunakan Media Animasi Pada Siswa Kelas I Dan II Di SDN 2 Gunaksa Tahun 2024
Authors: Saraswati Komang Putri , Putri Dwiastuti Sagung Agung , Ratih Ida Ayu Dewi Kumala , Arini Ni Wayan
Published: August 2024

Children in the educational phase are a vulnerable group to experience dental and oral health disorders due to their lack of knowledge and awareness to maintain their own dental health at this stage. One of the ways improve the knowledge is through health counseling. The purpose of this research is to determine the level of knowledge of students about dental and oral health before and after counseling using animation media on students of 1st and 2nd grade at SDN 2 Gunaksa in 2024. The method of research is a descriptive with the number 48 respondents, this research has been conducted in April 2024. The types of data used are primary data and secondary data. The data collected  method is through pre-test sheets before counseling, then counseling is given using animation media and post-test sheets after counseling which is then analyzed using univariate analysis. The research results shows that the percentage of students' knowledge about dental and oral health before counseling is highest at 37.50% in the good category and increases to 100% in the excellent category after counseling. The average level of knowledge before counseling is 62.08 with a sufficient category, increases to 89.79 with an excellent category after counseling. The conclusion of this study is that counseling using animation media can improve knowledge about dental and oral health.
Pengaruh Penambahan Kitosan Nano Gel Bermolekul Sedang pada Bahan Basis Gigi Tiruan Resin Akrilik Polimerisasi Panas Terhadap Kekuatan Transversal
Authors: Zulkarnain M. , Fitriana Triska
Published: August 2024

Hot polymerized acrylic resin is one of the most widely used denture base materials, but this material has low transverse strength so it is easy to fracture. Transverse strength can be increased by adding reinforcing material Chitosan nano gel which is proven to increase transverse strength because the Chitosan nano gel has special properties such as biodegradability, biocompatibility, good mechanical properties and good layer formation properties. The purpose of this study was to determine the effect of adding Chitosan nano gel to heat polymerized acrylic resin denture base material on the transverse strength. The research design used in this study was an experimental laboratory. The research sample was a rectangular hot polymerized acrylic resin with a size of 65 mm x 10 mm x 2.5 mm±0. 5 for the calculation of transverse strength with a total sample of 27 samples. The results of this study indicate that the value of transverse strength added with 1.25% Chitosan nano gel is the most effective concentration to increase the transverse strength of the heat polymerized acrylic resin denture base material compared to the addition of 1% Chitosan nano gel and without the addition of chitosan nano gel.
Educação Ambiental na manutenção dos serviços ecossistêmicos nas Cascatas de Namaacha – Província de Maputo
Authors: Tomo Regina Ruth Armindo , Tivane Adélia Luís
Published: August 2024

O estudo analisa o contributo da Educação Ambiental (EA) para a manutenção dos serviços ecossistêmicos (SE) nas cascatas de Namaacha a partir de uma abordagem qualitativa que incluiu entrevistas semiestruturadas e observação. A amostra foi de 45 moradores e utentes das cascatas de Namaacha selecionados com base na amostragem não probabilística por conveniência. Os dados coletados foram analisados e revelam que mesmo havendo esforços para a manutenção das cascatas¸ ainda há necessidade de inclusão de conhecimentos e boas práticas ambientais. Assim, a EA tem sim um papel importante na manutenção dos SE do local¸ visto que pode auxiliar na disseminação de conhecimentos, valores e desenvolvimento de ações para a mudança de atitudes face à destruição dos SE nas cascatas da Namaacha.
Accepted boundaries for barrier-free communication between autistic adults and non-autistic professionals
Authors: Mezőlaki Regina , Ivaskó Lívia
Published: August 2024

In this research, we explored our autistic and non-autistic subjects’ views on interactions and their interpretations of successful communication. We conducted qualitative analysis on semi-structured interviews with autistic adults requiring support (level 1) and professionals working with autistic people. Based on our results, we can argue that autistic and non-autistic individuals may similarly seek to construct a mutually successful interaction, as presupposed by the dynamic nature of communication. Both autistic and non-autistic people might have multiple challenges in an interaction. Limitations and strategies characterising both groups contribute to the social model of Double Empathy Problem. Applying the terms of relevance theory, misunderstandings or failures of communication might happen in an interaction involving different neurotyped interlocutors unless extra cognitive effort is invested in the needed communicational strategies at a metapragmatic level. Barrier-free communication provides equal opportunities and can result in mutual understanding between people with complex communication needs and neurotypical people.
OBTAINING PLANTING MATERIAL FOR KAMCHATKA BILBERRY ( LAMB.) BY CLONAL MICROPROPAGATION
Authors: Чудецкий Антон Игоревич , Кузнецова Ирина Борисовна , Макаров Сергей Сергеевич , Суров Владимир Викторович
Published: August 2024

В статье приведены результаты исследований по изучению влияния состава питательной среды и добавления регуляторов роста цитокининовой и ауксиновой групп в различных концентрациях на биометрические показатели растений красники (Vaccinium praestans Lamb.) при клональном микроразмножении. Клональное микроразмножение является наиболее эффективно для получения высококачественного оздоровленного посадочного материала при выращивании за пределами ареала произрастания. На этапе «собственно микроразмножение» показано влияние состава питательной среды (WPM и WPM 1/2), а также концентрации 6-БАП (0,5 и 1,0 мг/л) на количество и длину побегов растений красники. Максимальные количество (4,7 шт.) и суммарная длина (18,3 см) микропобегов отмечены в варианте WPM 1/2 + 6-БАП 0,5 мг/л. На этапе «укоренение in vitro» показано влияние состава питательной среды и концентрации ИМК (0,5 и 1,0 мг/л) на количество и длину корней. Максимальная суммарная длина корней (7,7 см) отмечена в варианте WPM 1/2 + ИМК 0,5 мг/л. Количество и суммарная длина микропобегов и корней красники на питательной среде WPM 1/2 были больше, чем на среде WPM.

The results of research to study the effect of the composition of the nutrient medium and the addition of growth regulators of the cytokinin and auxin groups at various concentrations on the biometric parameters of the Kamchatka bilberry (Vaccinium praestans Lamb.) plants during clonal micropropagation. Clonal micropropagation is most effective for obtaining high quality, healthy planting material when grown outside the growing area. The influence of the nutrient medium composition (WPM, WPM 1/2) and the concentration of 6-BAP (0.5 and 1.0 mg/l) on the number and length of shoots of Kamchatka bilberry plants at the stage “proper micropropagation”. The maximum number (4.7 pcs.) and total length (18.3 cm) of microshoots is noted in the WPM 1/2 + 6-BAP 0.5 mg/l. The influence of the nutrient medium composition and the concentration of IMC (0.5 and 1.0 mg/l) on the number and length of roots at the stage of “rooting in vitro”. The maximum total root length (7.7 cm) is noted in the WPM 1/2 + IMC 0.5 mg/l. The number and total length of microshoots and roots of the Kamchatka bilberry on the WPM 1/2 culture medium is greater than on the WPM.
INFLUENCE OF LIGHT ON THE SHOOT FORMATION AND RHIZOGENESIS OF COWBERRY IN CLONAL MICROPROPAGATION
Authors: Кузнецова Ирина Борисовна , Чудецкий Антон Игоревич , Тяк Галина Вячеславовна
Published: August 2024

В статье приведены результаты исследований по изучению влияния типа освещения различного спектрального диапазона на рост и развитие растений брусники обыкновенной (Vaccinium vitis-idaea L.) сортов Рубин и Костромская розовая при клональном микроразмножении. Применение метода клонального микроразмножения целесообразно при создания плантаций лесных ягодных растений для повышения коэффициента их размножения. Применение различного спектрального состава и спектрального диапазона света позволяет регулировать процессы роста и развития растений. На этапах «собственно микроразмножение» и «укоренение in vitro» количество микропобегов (3,3 шт.) и корней (3,9 шт.) брусники обыкновенной, а наибольшие суммарные длины микропобегов (14,9 см) и корней (15,5 см) при освещении надземной части растений-регенерантов светодиодными лампами с комбинацией белого, красного и синего спектров также были максимальными и значительно превышали показатели освещения люминесцентными и светодиодными лампами белого спектра. Суммарная длина микропобегов (10,7 см) и корней (8,6 см) брусники обыкновенной сорта Костромская розовая была значительно больше, чем у сорта Рубин.

The results of studies on the influence of the type of lighting of different spectral ranges on the growth and development of cowberry plants (Vaccinium vitis-idaea L.) of Rubin and Kostromskaya rozovaya cultivars during clonal micropropagation. The use of the method of clonal micropropagation is advisable when creating plantations of forest berry plants to increase the coefficient of its reproduction. The use of different spectral composition and spectral range of light makes it possible to regulate the processes of plant growth and development. The number of micro-shoots (3.3 pcs.) and roots (3.9 pcs.) of lingonberry, as well as the total length of micro-shoots (14.9 cm) and roots (15.5 cm) are observed when the aboveground part of the regenerated plants are illuminated with LED lamps with a combination of white, red and blue spectra, are also more maximal and significantly exceeded the illumination rates of fluorescent and LED lamps of the white spectrum at the stages “proper micropropagation” and “rooting in vitro”. The total length of micro-shoots (10.7 cm) and roots (8.6 cm) of lingonberry of Kostromskaya rozovaya cultivar is significantly greater than that of the Rubin variety.
INFLUENCE OF LIGHT OF VARIOUS SPECTRAL RANGES ON BIOMETRIC PARAMETERS OF ARCTIC BRAMBLE (L.) PLANTS IN CLONAL MICROPROPAGATION
Authors: Макаров Сергей Сергеевич , Кузнецова Ирина Борисовна
Published: August 2024

В статье приведены результаты исследований по изучению влияния освещения различного спектрального диапазона на биометрические показатели роста растений княженики арктической (Rubus arcticus L.) сорта Anna и перспективной гибридной формы К-1 при клональном микроразмножении. Княженика арктическая - одна из наиболее перспективных ягодных культур для выращивания в условиях выработанных торфяников. Клональное микроразмножение - наиболее эффективный метод размножения для создания плантаций лесных ягодных растений. На этапе «собственно микроразмножение» при освещении растений-регенерантов княженики арктической лампами с комбинацией белого, красного и синего спектров формировалось наибольшее количество микропобегов (16,9 шт.) и максимальной суммарной длиной (251,1 см). Суммарная длина микропобегов княженики сорта Anna (114,0 см) была больше, чем у растений гибридной формы К-1. На этапе «укоренение in vitro» корнеобразование княженики арктической при освещении растений светодиодными лампами с комбинацией белого, красного и синего спектров было более активным (количество корней -13,4 шт., суммарная длина корней - 163,8 см), чем при использовании люминесцентных ламп и светодиодных ламп белого спектра. Суммарная длина растений княженики гибридной формы К-1 (84,4 см) была больше по сравнению с растениями сорта Anna.

The results of research on the study of the effect of illumination of different spectral ranges on the biometric parameters of growth of the arctic bramble (Rubus arcticus L.) plants of Anna cultivar and the promising hybrid form K-1 during clonal micropropagation. The arctic bramble is one of the most promising berry crops for cultivation in the conditions of depleted peatlands. Clonal micropropagation is the most effective propagation method for creating forest berry plantations. The largest number of microshoots (16.9 pcs.) and the maximum total length (251.1 cm) of regenerating arctic bramble plants during illumination with LED lamps with a combination of white, red and blue spectra at the stage of “proper micropropagation”. The total length of microshoots of the arctic bramble of Anna cultivar (114.0 cm) is greater than hybrid form K-1. The rooting of the arctic bramble plants under the illumination with LED lamps with a combination of white, red and blue spectra is more active (the number of roots is 13.4 pcs., the total length of the roots is 163.8 cm) at the stage of “rooting in vitro” than when using luminescent lamps and LED lamps of the white spectrum. The total length of the arctic bramble plants of the K-1 hybrid form (84.4 cm) is greater than Anna cultivar.
EFFECT OF LANTANUM ON THE YIELD AND CHEMICAL COMPOSITION OF CORNS HERBAGE
Authors: Сыренжапова Арюна Сыдынжаповна , Хубракова Бэлигма Цыденбаловна , Буянтуева Аик Владимировна
Published: August 2024

В статье рассматриваются вопросы влияния лантана в разных дозах на химический состав и продуктивность кукурузы гибрида Росс-191 М. Приведены материалы, полученные в вегетационных опытах на кафедре почвоведения и агрохимии ФГБОУ ВО «Бурятская ГСХА им. В.Р. Филиппова» в 2015-2018 годах. В исследованиях показано положительное влияние лантана в дозах 3 и 6 мг на кг почвы на накопление азота, клетчатки, сахара, золы в надземной массе и урожайность испытуемой культуры. Наибольшая продуктивность при внесении лантана отмечалась в дозах 3, 6 и 9 мг и составила 22,1; 21,4 и 14,7 % соответственно по отношению к контролю. Результаты исследований показали, что в листьях больше накапливается клетчатка, общий азот, фосфор, калий и зола, а в стеблях преобладало накопление сахаров. В наших опытах синтез углерода органического вещества в листьях кукурузы осуществлялся наиболее активно в вариантах с дозой 3 и 6 мг лантана и составлял 3,24 и 2,72 мг/дм∙ час соответственно. При более высоких дозах 9 и 12 мг La синтез углерода органического вещества снижался. Результаты исследований показали, что применение лантана в форме сульфата способствовало повышению продуктивности кукурузы, улучшению качества его зеленой массы за счет большего содержания азота, клетчатки, сахаров и минеральных элементов в надземной его части.

The article discusses the influence of lanthanum in different doses on the chemical composition and productivity of corn of the Ross-191 M variety. The materials obtained in vegetation experiments at the Department of Soil Science and Agrochemistry of the Buryat State Agricultural Academy named after V.R. Filippov in 2015-2018 are presented. Studies have shown a positive effect of lanthanum in doses of 3 and 6 mg per kg of soil on the accumulation of nitrogen, fiber, sugar, ash in the aboveground mass and the yield of the tested crop. The increase in yield when applying lanthanum was observed at doses of 3, 6 and 9 mg and amounted to 22.1; 21.4 and 14.7%, respectively, in relation to the control. The results of the studies showed that fiber, total nitrogen, phosphorus, potassium and ash accumulate more in the leaves, and the accumulation of sugars in the stems prevailed. In our experiments, the synthesis of carbon of organic matter in corn leaves was carried out most actively in variants with a dose of 3 and 6 mg of lanthanum and amounted to 3.24 and 2.72 mg/dm2 * hour, respectively. At higher doses of 9 and 12 mg La, carbon synthesis of organic matter decreased. The results of the research showed that the use of lanthanum in the form of sulfate contributed to an increase in the yield of corn, improving the quality of its green mass due to a higher content of nitrogen, fiber, sugars and mineral elements in its aboveground part.
ADAPTATION OF STATISTICAL METHODS BASED ON BLUP SIRE MODEL ASSESSING THE BREEDING VALUE OF SIRES IN BEEF CATTLE BREEDING
Authors: Хайнацкий Валерий Юрьевич , Акимов Сергей Сергеевич
Published: August 2024

Уровень селекционной работы в мясном скотоводстве, в том числе и оценка племенной ценности животных в Российской Федерации, существенно ниже по сравнению с зарубежными странами, где отдается предпочтение применению программ селекции, основанных на прогнозе племенной генетической ценности животных. Получение достоверных значений племенной ценности по селекционируемым признакам играет ключевую роль в создании эффективной системы оценки. В основе зарубежных программ лежит процедура наилучшего линейного несмещенного прогноза, разработанного в США профессором Хендерсоном, признанным лидером и авторитетом по генетической оценке животных и разработке анализа несбалансированных данных линейными моделями смешанного типа. В статье показана значимость метода BLUP Sire Model при определении племенной ценности производителей в мясном скотоводстве. Представлены основные принципы построения работоспособной системы оценки племенной ценности в современном формате на базе простейшей модели, порядок построения линейных биометрических моделей, преобразование уравнений наименьших квадратов в уравнения смешанной модели и расчет точности проводимой оценки. Дана краткая описательная характеристика прототипа разрабатываемой программы, основанной на использовании Excel, базовые команды которой предоставляют широкие возможности для расчета самых разнообразных показателей, в том числе и по методике BLUP Sire Model.

The level of breeding work in beef cattle breeding including the assessment of breeding value of animals in the Russian Federation is significantly lower than in foreign countries, where preference is given to the use of breeding programs based on the forecast of breeding genetic value of animals. Obtaining reliable breeding values for breeding traits plays a key role in creating an effective assessment system. The foreign programs are based on the Best Linear Unbiased Prediction, developed in the USA by Professor Henderson, a recognized leader and authority in the genetic evaluation of animals and the development of analysis of unbalanced data by linear mixed-type models. The article shows the significance of BLUP Sire Model method in determining the breeding value of sires in beef cattle breeding. The basic principles of building a workable breeding value assessment system in a modern format based on the simplest model, the procedure for constructing linear biometric models; transforming least square equations into mixed model equations and calculating the accuracy of the assessment are presented. A brief descriptive characteristic of the prototype of the developed program based on the use of Excel is given, the basic commands of which provide ample opportunities for calculating a wide variety of indicators, including the BLUP Sire Model method.
NORMALIZATION OF PHYSIOLOGICAL PROCESSES UNDER PHYSICAL LOADING IN THE BACKGROUND OF ADAPTOGEN APPLICATION
Authors: Хабибуллин Рузель Муллахметович , Хабибуллин Ильмир Муллахметович , Миронова Ирина Валерьевна
Published: August 2024

Наши экспериментальные исследования проводились с целью восстановления физиологических функций организма модельных животных после максимальных физических нагрузкок при использовании в рационе животных биологически активных веществ и дальнейшего применения полученных результатов в практике спортивных тренировок в силовой гимнастике. Для восстановления постоянства внутренней среды организма лабораторных животных после максимальных физических нагрузок нами впервые предложено использование биологически активных веществ в рационе животных. Применение биологически активных веществ различной природы приводило организм лабораторных животных к оптимизации гомеостаза, которое оказывало положительное влияние на скелетную мускулатуру, сердечную мышцу, легочную ткань, печень и почки, в которых происходят процессы формирования иммунитета и образования клеток крови, что было установлено в наших экспериментах. На фоне максимальных физических нагрузок применение биологически активных веществ таких как настойка лимонника, левзеи, трутневого молочка, пантокрина позволяют увеличить работоспособность животных. Кроме того, наблюдается стабилизация гомеостаза организма животных опытных групп, что выражается в активизации физиологического статуса, а также восстановлении морфологических структур органов.

Our experimental studies were carried out with the aim of restoring the physiological functions of the organism of model animals after maximum physical exertion when using biologically active substances in the diet of animals and further applying the results obtained in the practice of sports training in strength gymnastics. To restore the constancy of the internal environment of the organism of laboratory animals after maximum physical exertion, we for the first time proposed the use of biologically active substances in the diet of animals. The use of biologically active substances of various natures led the organism of laboratory animals to optimize homeostasis, which had a positive effect on skeletal muscles, heart muscle, lung tissue, liver and kidneys in which the processes of formation of immunity and formation of blood cells take place, which was established in our experiments. Against the background of maximum physical exertion, the use of biologically active substances such as tincture of lemongrass, leuzea, drone milk, pantocrine can increase the efficiency of animals. In addition, the stabilization of the homeostasis of the organism of the animals of the experimental groups is observed, which is expressed in the activation of the physiological status, as well as the restoration of the morphological structures of the organs.
VACCINIUM PRAESTANS IN VITRO EX VITRO
Authors: Чудецкий Антон Игоревич , Родин Сергей Анатольевич , Кузнецова Ирина Борисовна , Макаров Сергей Сергеевич
Published: August 2024

В статье приведены результаты исследований по изучению влияния субстрата, обработки современным ростостимулярующими препаратами и мульчирования на процесс адаптации растений красники (Vaccinium praestans Lamb.) in vitro к нестерильным условиям ex vitro. В последнее время происходит интенсивное уменьшение запасов естественных угодий ценных видов лесных ягодных растений. Красника - малоизвестное в европейской части России лесное ягодное растение с ограниченным ареалом естественного произрастания. Для промышленного выращивания лесных ягодных растений целесообразно использовать метод микроклонального размножения. Адаптация к нестерильным условиям ex vitro - один из самых ответственных и сложных этапов микроклонального размножения. На этапе адаптации красники in vitro к нестерильным условиям ex vitro оптимальным сроком пересадки является май. Наибольшая приживаемость (90-93%) отмечена на смеси торфа с песком 1:1 с обработкой препаратом HB-101 0,1 мл/л и опрыскиванием водой, на верховом торфе и смеси торфа с вермикулитом 1:4 с обработкой препаратом HB-101 0,1 мл/л. Максимальное количество побегов (3,1-3,9 шт.) и листьев (4,5-4,9 шт.) красники ex vitro выявлено при обработке препаратом HB-101 0,1 мл/л. Приживаемость растений красники с мульчированием посадок мхом Sphagnum L. оказалась незначительно выше, чем в вариантах без мульчирования.

The article deals with the results of the research on the study of the effect of the substrate, treatment with modern growth-stimulating agents and mulching on the process of adaptation of Kamchatka bilberry (Vaccinium praestans Lamb.) plants in vitro to non-sterile ex vitro conditions. At present, an intensive decrease in the reserves of natural lands of valuable species of forest berry plants is observed. Kamchatka bilberry is a forest berry plant with a limited range of natural growth and almost unknown in the European part of Russia. For industrial cultivation of forest berry plants the method of clonal micropropagation is advisable. Adaptation to non-sterile ex vitro conditions is one of the most crucial and difficult stages of clonal micropropagation. At the stage of in vitro adaptation of Kamchatka bilberry to non-sterile ex vitro conditions the optimal transplantation period is May. The highest survival rate (90-93%) is observed on a mixture of peat with sand 1:1 with treatment with HB-101 0.1 ml/l and spraying with water, on high moor peat and a mixture of peat with vermiculite 1:4 with treatment with HB-101 0.1 ml/l. The maximum number of shoots (3.1-3.9 pcs.) and leaves (4.5-4.9 pcs.) ex vitro is revealed after treatment with HB-101 0.1 ml/l. The survival rate of Kamchatka bilberry plants with mulching of plantings with Sphagnum L. moss turned out to be slightly higher than in variants without mulching.