bluuebunny/crossref_metadata_2025 huggingface dataset:
Enzymology of Alternative Carbohydrate Catabolic Pathways
Authors: Kopp Dominik , Bergquist Peter L. , Sunna Anwar
Published: October 2020

The Embden–Meyerhof–Parnas (EMP) and Entner–Doudoroff (ED) pathways are considered the most abundant catabolic pathways found in microorganisms, and ED enzymes have been shown to also be widespread in cyanobacteria, algae and plants. In a large number of organisms, especially common strains used in molecular biology, these pathways account for the catabolism of glucose. The existence of pathways for other carbohydrates that are relevant to biomass utilization has been recognized as new strains have been characterized among thermophilic bacteria and Archaea that are able to transform simple polysaccharides from biomass to more complex and potentially valuable precursors for industrial microbiology. Many of the variants of the ED pathway have the key dehydratase enzyme involved in the oxidation of sugar derived from different families such as the enolase, IlvD/EDD and xylose-isomerase-like superfamilies. There are the variations in structure of proteins that have the same specificity and generally greater-than-expected substrate promiscuity. Typical biomass lignocellulose has an abundance of xylan, and four different pathways have been described, which include the Weimberg and Dahms pathways initially oxidizing xylose to xylono-gamma-lactone/xylonic acid, as well as the major xylose isomerase pathway. The recent realization that xylan constitutes a large proportion of biomass has generated interest in exploiting the compound for value-added precursors, but few chassis microorganisms can grow on xylose. Arabinose is part of lignocellulose biomass and can be metabolized with similar pathways to xylose, as well as an oxidative pathway. Like enzymes in many non-phosphorylative carbohydrate pathways, enzymes involved in L-arabinose pathways from bacteria and Archaea show metabolic and substrate promiscuity. A similar multiplicity of pathways was observed for other biomass-derived sugars such as L-rhamnose and L-fucose, but D-mannose appears to be distinct in that a non-phosphorylative version of the ED pathway has not been reported. Many bacteria and Archaea are able to grow on mannose but, as with other minor sugars, much of the information has been derived from whole cell studies with additional enzyme proteins being incorporated, and so far, only one synthetic pathway has been described. There appears to be a need for further discovery studies to clarify the general ability of many microorganisms to grow on the rarer sugars, as well as evaluation of the many gene copies displayed by marine bacteria.
A DFT Study for Catalytic Deoxygenation of Methyl Butyrate on a Lewis Acid Site of ZSM-5 Zeolite
Authors: Chen Xiaobo , Li Ruiying , Yan Hao , Liu Yibin , Yang Chaohe
Published: October 2020

The catalytic deoxygenation mechanism of fatty acid esters on a Lewis acid site of ZSM-5 zeolite was elucidated via density functional theory (DFT) by using a methyl butyrate (MB) as the model compound for fatty acid esters. The configurations of the initial reactant, transition states, and products together with the activation barrier of each elementary reaction were determined. The activation barrier of different initial cracking reactions decreases in the order of α-C–C > β-C–C > α-C–O > β-C–O. The best reaction path for catalytic deoxygenation of methyl butyrate over Lewis acid site is CH3CH2CH2C(OCH3)=O⋯Lewis → CH3CH2⋯Lewis⋯C(=CH2)OCH3 → CH2=CH2 + CH3COOCH3 + Lewis. The oxygen of methyl butyrate is mainly removed as CO2, methyl acetate, formaldehyde, and butyraldehyde, while ethylene, propylene, and butane are the main hydrocarbon products. In addition, the group generated by cracking of methyl butyrate form a bond with the Lewis acid site, promoting the transformation between a Lewis acid and a Brønsted acid. The corresponding intermediates have a high single point energy, but the poor stability leads to further deoxygenation and cracking reactions. This work provides a theoretical basis for the modification in the number of Brønsted acid and Lewis acid sites in the ZSM-5 zeolite.
Synthesis of Ni- and N-Doped Titania Nanotube Arrays for Photocatalytic Hydrogen Production from Glycerol–Water Solutions
Authors: Elysabeth Tiur , Agriyfani Dwi Annisa , Ibadurrohman Muhammad , Nurdin Muhammad , Slamet
Published: October 2020

Synthesis of Ni- and N-doped Titania Nanotube Arrays (Ni-N-TiNTAs) was conducted to produce photocatalysts for hydrogen production from a glycerol–water mixture. Ni-N-TiNTAs were synthesized in two steps, namely anodization and chemical reduction. Ti foil was anodized at 50 V for two h in an electrolyte solution containing 0.2% urea as a source of N atoms. Ni doping (at various content of 5%, 10%, 15%, and 20% wt) was performed by one-hour chemical reduction with sodium borohydride as a reducing agent. The photocatalyst was annealed at different temperatures, i.e., 500 °C, 550 °C, and 600 °C under 60 mL/min N2 gas for two h. On the basis of X-ray Diffraction (XRD) patterns, Ni-N-TiNTAs are mostly of anatase crystallite phase when annealed at 500 °C and 550 °C, while that of rutile was observable when calcination was done at 600 °C. The morphology of the photocatalysts was scrutinized by means of Field Emission Scanning Electron Microscopy (FESEM) imaging, which reveals nanotubular structures, with elemental composition measured by Energy Dispersive X-ray (EDX). The bandgap of the photocatalysts was analyzed using Ultraviolet Diffuse Reflectance Spectroscopy (UV DRS), which showed a lower value for the case of Ni-N-TiNTAs as compared to those of TiNTAs and N-TiNTAs. Photocatalytic tests showed that the highest amount of hydrogen produced (ca. 30973 μmol/m2) was obtained in the case of Ni-N-TiNTAs with a Ni content of 10wt%.
Potential Drug Candidates to Treat TRPC6 Channel Deficiencies in the Pathophysiology of Alzheimer’s Disease and Brain Ischemia
Authors: Prikhodko Veronika , Chernyuk Daria , Sysoev Yurii , Zernov Nikita , Okovityi Sergey , Popugaeva Elena
Published: October 2020

Alzheimer’s disease and cerebral ischemia are among the many causative neurodegenerative diseases that lead to disabilities in the middle-aged and elderly population. There are no effective disease-preventing therapies for these pathologies. Recent in vitro and in vivo studies have revealed the TRPC6 channel to be a promising molecular target for the development of neuroprotective agents. TRPC6 channel is a non-selective cation plasma membrane channel that is permeable to Ca2+. Its Ca2+-dependent pharmacological effect is associated with the stabilization and protection of excitatory synapses. Downregulation as well as upregulation of TRPC6 channel functions have been observed in Alzheimer’s disease and brain ischemia models. Thus, in order to protect neurons from Alzheimer’s disease and cerebral ischemia, proper TRPC6 channels modulators have to be used. TRPC6 channels modulators are an emerging research field. New chemical structures modulating the activity of TRPC6 channels are being currently discovered. The recent publication of the cryo-EM structure of TRPC6 channels should speed up the discovery process even more. This review summarizes the currently available information about potential drug candidates that may be used as basic structures to develop selective, highly potent TRPC6 channel modulators to treat neurodegenerative disorders, such as Alzheimer’s disease and cerebral ischemia.
SI: Survivability under Overheating: The Impact of Regional and Global Climate Change on the Vulnerable and Low-Income Population
Authors: Synnefa Afroditi , Haddad Shamila , Rajagopalan Priyadarsini , Santamouris Mattheos
Published: October 2020

The present special issue discusses three significant challenges of the built environment, namely regional and global climate change, vulnerability, and survivability under the changing climate. Synergies between local climate change, energy consumption of buildings and energy poverty, and health risks highlight the necessity to develop mitigation strategies to counterbalance overheating impacts. The studies presented here assess the underlying issues related to urban overheating. Further, the impacts of temperature extremes on the low-income population and increased morbidity and mortality have been discussed. The increasing intensity, duration, and frequency of heatwaves due to human-caused climate change is shown to affect underserved populations. Thus, housing policies on resident exposure to intra-urban heat have been assessed. Finally, opportunities to mitigate urban overheating have been proposed and discussed.
Differential Gene Expression in Brain and Liver Tissue of Wistar Rats after Rapid Eye Movement Sleep Deprivation
Authors: Pandey Atul , Oliver Ryan , Kar Santosh K
Published: October 2020

Sleep is essential for the survival of most living beings. Numerous researchers have identified a series of genes that are thought to regulate “sleep-state” or the “deprived state”. As sleep has a significant effect on physiology, we believe that lack of total sleep, or particularly rapid eye movement (REM) sleep, for a prolonged period would have a profound impact on various body tissues. Therefore, using the microarray method, we sought to determine which genes and processes are affected in the brain and liver of rats following nine days of REM sleep deprivation. Our findings showed that REM sleep deprivation affected a total of 652 genes in the brain and 426 genes in the liver. Only 23 genes were affected commonly, 10 oppositely, and 13 similarly across brain and liver tissue. Our results suggest that nine-day REM sleep deprivation differentially affects genes and processes in the brain and liver of rats.
Spin Coating Immobilisation of C-N-TiO2 Co-Doped Nano Catalyst on Glass and Application for Photocatalysis or as Electron Transporting Layer for Perovskite Solar Cells
Authors: Mouele Emile Salomon Massima , Ngqoloda Siphelo , Pescetelli Sara , Di Carlo Aldo , Dinu Mihaela , Vladescu Alina , Parau Anca Constantina , Agresti Antonio , Braic Mariana , Arendse Christopher J. , Petrik Leslie Felicia
Published: October 2020

Producing active thin films coated on supports resolves many issues of powder-based photo catalysis and energy harvesting. In this study, thin films of C-N-TiO2 were prepared by dynamic spin coating of C-N-TiO2 sol-gel on glass support. The effect of spin speed and sol gel precursor to solvent volume ratio on the film thickness was investigated. The C-N-TiO2-coated glass was annealed at 350 °C at a ramping rate of 10 °C/min with a holding time of 2 hours under a continuous flow of dry N2. The C-N-TiO2 films were characterised by profilometry analysis, light microscopy (LM), and scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS). The outcomes of this study proved that a spin coating technique followed by an annealing process to stabilise the layer could be used for immobilisation of the photo catalyst on glass. The exposure of C-N-TiO2 films to UV radiation induced photocatalytic decolouration of orange II (O.II) dye. The prepared C-N-TiO2 films showed a reasonable power conversion efficiency average (PCE of 9%) with respect to the reference device (15%). The study offers a feasible route for the engineering of C-N-TiO2 films applicable to wastewater remediation processes and energy harvesting in solar cell technologies.
Liquid Crystal Peptide/DNA Coacervates in the Context of Prebiotic Molecular Evolution
Authors: Jia Tony Z. , Fraccia Tommaso P.
Published: October 2020

Liquid–liquid phase separation (LLPS) phenomena are ubiquitous in biological systems, as various cellular LLPS structures control important biological processes. Due to their ease of in vitro assembly into membraneless compartments and their presence within modern cells, LLPS systems have been postulated to be one potential form that the first cells on Earth took on. Recently, liquid crystal (LC)-coacervate droplets assembled from aqueous solutions of short double-stranded DNA (s-dsDNA) and poly-L-lysine (PLL) have been reported. Such LC-coacervates conjugate the advantages of an associative LLPS with the relevant long-range ordering and fluidity properties typical of LC, which reflect and propagate the physico-chemical properties of their molecular constituents. Here, we investigate the structure, assembly, and function of DNA LC-coacervates in the context of prebiotic molecular evolution and the emergence of functional protocells on early Earth. We observe through polarization microscopy that LC-coacervate systems can be dynamically assembled and disassembled based on prebiotically available environmental factors including temperature, salinity, and dehydration/rehydration cycles. Based on these observations, we discuss how LC-coacervates can in principle provide selective pressures effecting and sustaining chemical evolution within partially ordered compartments. Finally, we speculate about the potential for LC-coacervates to perform various biologically relevant properties, such as segregation and concentration of biomolecules, catalysis, and scaffolding, potentially providing additional structural complexity, such as linearization of nucleic acids and peptides within the LC ordered matrix, that could have promoted more efficient polymerization. While there are still a number of remaining open questions regarding coacervates, as protocell models, including how modern biologies acquired such membraneless organelles, further elucidation of the structure and function of different LLPS systems in the context of origins of life and prebiotic chemistry could provide new insights for understanding new pathways of molecular evolution possibly leading to the emergence of the first cells on Earth.
Development of Co(OH)xF2−x Nanosheets for Acetone Gas Sensor Applications: Material Characterization and Sensor Performance Evaluation
Authors: Yan Yaping , Eom Tae-yil , Xu Shiyu , Yoo Pil J. , Yan Changzeng , Park Joon-Shik , Lee Hoo-Jeong
Published: October 2020

This study reports the employment of Co(OH)xF2−x nanosheets, a new material in the sensor field, for gas sensor applications. We synthesize Co(OH)xF2−x nanosheets via a hydrothermal route using SiO2 sphere templates. Our material characterization confirms that the material is a densely clustered Co(OH)xF2−x nanosheet with an amorphous microstructure with some short-range ordering. Sensors based on the nanosheets demonstrate a high response of 269% toward 4.5 ppm of acetone gas at an operation temperature of 200 °C and a very low minimum detection limit of 40 ppb. It functions effectively up to a temperature below 300 °C, above which F is found to start to evaporate. Our discussion suggests that an excellent sensor performance arises from the high catalytic function of F incorporated in a high concentration in the material as well as the high specific surface area due to the morphology of densely clustered nanosheets.
Early Diagnosis of Pancreatic Cancer: The Key for Survival
Authors: Gheorghe Gina , Bungau Simona , Ilie Madalina , Behl Tapan , Vesa Cosmin Mihai , Brisc Ciprian , Bacalbasa Nicolae , Turi Vladiana , Costache Raluca Simona , Diaconu Camelia Cristina
Published: October 2020

Pancreatic cancer (PC) is one of the most aggressive forms of cancer. Negative prognosis is mainly due to the late diagnosis in advanced stages, when the disease is already therapeutically overcome. Studies in recent years have focused on identifying biomarkers that could play a role in early diagnosis, leading to the improvement of morbidity and mortality. Currently, the only biomarker widely used in the diagnosis of PC is carbohydrate antigen 19-9 (CA19.9), which has, however, more of a prognostic role in the follow-up of postoperative recurrence than a diagnostic role. Other biomarkers, recently identified as the methylation status of ADAMTS1 (A disintegrin and metalloproteinase with thrombospondin motifs 1) and BNC1 (zinc finger protein basonuclin-1) in cell-free deoxyribonucleic acid (DNA), may play a role in the early detection of PC. This review focuses on the diagnosis of PC in its early stages.
Dual-Energy Computed Tomography of the Lung in COVID-19 Patients: Mismatch of Perfusion Defects and Pulmonary Opacities
Authors: Afat Saif , Othman Ahmed E. , Nikolaou Konstantin , Gassenmaier Sebastian
Published: October 2020

To evaluate contrast-enhanced dual-energy computed tomography (DECT) chest examinations regarding pulmonary perfusion patterns and pulmonary opacities in patients with confirmed COVID-19 disease. Fourteen patients with 24 DECT examinations performed between April and May 2020 were included in this retrospective study. DECT studies were assessed independently by two radiologists regarding pulmonary perfusion defects, using a Likert scale ranging from 1 to 4. Furthermore, in all imaging studies the extent of pulmonary opacities was quantified using the same rating system as for perfusion defects. The main pulmonary findings were ground glass opacities (GGO) in all 24 examinations and pulmonary consolidations in 22 examinations. The total lung scores after the addition of the scores of the single lobes showed significantly higher values of opacities compared to perfusion defects, with a median of 12 (9–18) for perfusion defects and a median of 17 (15–19) for pulmonary opacities (p = 0.002). Furthermore, mosaic perfusion patterns were found in 19 examinations in areas with and without GGO. Further studies will be necessary to investigate the pathophysiological background of GGO with maintained perfusion compared to GGO with reduced perfusion, especially regarding long-term lung damage and prognosis.
Personalization of Aspirin Therapy Ex Vivo in Patients with Atherosclerosis Using Light Transmission Aggregometry
Authors: Khan Hamzah , Gallant Reid C. , Zamzam Abdelrahman , Jain Shubha , Afxentiou Sherri , Syed Muzammil , Kroezen Zachary , Shanmuganathan Meera , Britz-McKibbin Philip , Rand Margaret L. , Ni Heyu , Al-Omran Mohammed , Qadura Mohammad
Published: October 2020

Acetylsalicylic acid (ASA), also known as aspirin, appears to be ineffective in inhibiting platelet aggregation in 20–30% of patients. Light transmission aggregometry (LTA) is a gold standard platelet function assay. In this pilot study, we used LTA to personalize ASA therapy ex vivo in atherosclerotic patients. Patients were recruited who were on 81 mg ASA, presenting to ambulatory clinics at St. Michael’s Hospital (n = 64), with evidence of atherosclerotic disease defined as clinical symptoms and diagnostic findings indicative of symptomatic peripheral arterial disease (PAD), with an ankle brachial index (ABI) of <0.9 (n = 52) or had diagnostic features of asymptomatic carotid arterial stenosis (CAS), with >50% stenosis of internal carotid artery on duplex ultrasound (n = 12). ASA compliance was assessed via multisegmented injection-capillary electrophoresis-mass spectrometry based on measuring the predominant urinary ASA metabolite, salicyluric acid. LTA with arachidonic acid was used to test for ASA sensitivity. Escalating ASA dosages of 162 mg and 325 mg were investigated ex vivo for ASA dose personalization. Of the 64 atherosclerotic patients recruited, 8 patients (13%) were non-compliant with ASA. Of ASA compliant patients (n = 56), 9 patients (14%) were non-sensitive to their 81 mg ASA dosage. Personalizing ASA therapy in 81 mg ASA non-sensitive patients with escalating dosages of ASA demonstrated that 6 patients became sensitive to a dosage equivalent to 162 mg ASA and 3 patients became sensitive to a dosage equivalent to 325 mg ASA. We were able to personalize ASA dosage ex vivo in all ASA non-sensitive patients with escalating dosages of ASA within 1 h of testing.
Allele-Specific PCR for KRAS Mutation Detection Using Phosphoryl Guanidine Modified Primers
Authors: Chubarov Alexey S. , Oscorbin Igor P. , Filipenko Maxim L. , Lomzov Alexander A. , Pyshnyi Dmitrii V.
Published: October 2020

Establishing the Kirsten rat sarcoma (KRAS) mutational status is essential in terms of managing patients with various types of cancer. Allele-specific real-time polymerase chain reaction (AS-PCR) is a widely used method for somatic mutations detection. To improve the limited sensitivity and specificity, several blocking methods have been introduced in AS-PCR to block the amplification of wild-type templates. Herein, we used a novel modified oligonucleotide with internucleotide phosphates reshaped 1,3-dimethyl-2-imino-imidazolidine moieties (phosphoryl guanidine (PG) groups) as primers and blockers in the AS-PCR method. Four common KRAS mutations were chosen as a model to demonstrate the advantages of the PG primers and blockers utilizing a customized PCR protocol. The methods were evaluated on plasmid model systems providing a KRAS mutation detection limit of 20 copies of mutant DNA in a proportion as low as 0.1% of the total DNA, with excellent specificity. PG-modification can serve as the universal additional mismatch-like disturbance to increase the discrimination between wild-type and mutated DNA. Moreover, PG can serve to increase primer specificity by a synergetic effect with additional mismatch and would greatly facilitate medical research.
Integrating Datasets on Public Health and Clinical Aspects of Sickle Cell Disease for Effective Community-Based Research and Practice
Authors: Isokpehi Raphael D. , Johnson Chomel P. , Tucker Ashley N. , Gautam Aakriti , Brooks Taylor J. , Johnson Matilda O. , Cozart Thometta , Wathington Deanna J.
Published: October 2020

Sickle cell disease (SCD) is a genetic disease that has multiple aspects including public health and clinical aspects. The goals of the research study were to (1) understand the public health aspects of sickle cell disease, and (2) understand the overlap between public health aspects and clinical aspects that can inform research and practice beneficial to stakeholders in sickle cell disease management. The approach involved the construction of datasets from textual data sources produced by experts on sickle cell disease including from landmark publications published in 2020 on sickle cell disease in the United States. The interactive analytics of the integrated datasets that we produced identified that community-based approaches are common to both public health and clinical aspects of sickle cell disease. An interactive visualization that we produced can aid the understanding of the alignment of governmental organizations to recommendations for addressing sickle cell disease in the United States. From a global perspective, the interactive analytics of the integrated datasets can support the knowledge transfer stage of the SICKLE recommendations (Skills transfer, Increasing self-efficacy, Coordination, Knowledge transfer, Linking to adult services, and Evaluating readiness) for effective pediatric to adult transition care for patients with sickle cell disease. Considering the increased digital transformations resulting from the COVID-19 pandemic, the constructed datasets from expert recommendations can be integrated within remote digital platforms that expand access to care for individuals living with sickle cell disease. Finally, the interactive analytics of integrated expert recommendations on sickle cell disease management can support individual and team expertise for effective community-based research and practice.
Constructal Equivalent Thermal Resistance Minimization for Tau-Shaped Fin
Authors: Wei Shuhuan , Feng Huijun , Chen Lingen , Ge Yanlin
Published: October 2020

With the aid of constructal theory and entransy theory, a Tau-shaped fin (TAUSF) is investigated in this paper, and the widths of the bend end and elemental fins are assumed to be different. The construct of the TAUSF is optimized by the minimum equivalent thermal resistance (ETR) obtained by entransy dissipation rate. The constraints of total enveloping volume and fin material volume are considered. The results show that in the specified range of width ratio, the twice minimum ETR of the TAUSF can be yielded by an optimal width ratio and an optimal length ratio. In addition, comparing the optimal performance of the TAUSF with the counterpart of a T-shaped fin, the former sacrifices a small amount of heat transfer performance and its stiffness increases due to its structure with the bend end. The optimal structure of the TAUSF yielded from ETR minimization is conspicuously different with the counterpart yielded from maximum thermal resistance minimization. Comparing the thermal performances of the two optimal constructs, the ETR of the former optimal construct is declined by 10.58%, whereas the maximum thermal resistance is augmented by 5.22%. The former optimal construct can lead to the uniformity of temperature gradient and the reduction in thermal stress, and can guide the engineering designs of practical fins.
Thermodynamic Curvature of the Binary van der Waals Fluid
Authors: Ruppeiner George , Seftas Alex
Published: October 2020

The thermodynamic Ricci curvature scalar R has been applied in a number of contexts, mostly for systems characterized by 2D thermodynamic geometries. Calculations of R in thermodynamic geometries of dimension three or greater have been very few, especially in the fluid regime. In this paper, we calculate R for two examples involving binary fluid mixtures: a binary mixture of a van der Waals (vdW) fluid with only repulsive interactions, and a binary vdW mixture with attractive interactions added. In both of these examples, we evaluate R for full 3D thermodynamic geometries. Our finding is that basic physical patterns found for R in the pure fluid are reproduced to a large extent for the binary fluid.
Online Delivery and Assessment during COVID-19: Safeguarding Academic Integrity
Authors: Gamage Kelum A.A. , Silva Erandika K. de , Gunawardhana Nanda
Published: October 2020

Globally, the number of COVID-19 cases continues to rise daily despite strict measures being adopted by many countries. Consequently, universities closed down to minimise the face-to-face contacts, and the majority of the universities are now conducting degree programmes through online delivery. Remote online delivery and assessment are novel experiences for many universities, which presents many challenges, particularly when safeguarding academic integrity. For example, invigilated assessments, often considered as more secure, are not an option given the current situation and detecting any cheating would be significantly challenging. This paper reviews assessment security in the digital domain and critically evaluates the practices from different universities in safeguarding academic integrity, including associated challenges.
Numerical Simulation of Shearer Operation in a Longwall District
Authors: Dziurzyński Wacław , Krach Andrzej , Krawczyk Jerzy , Pałka Teresa
Published: October 2020

This paper presents a relatively simple method to analyze potential methane hazard and preventive methods based on a computer simulation of the airflow and methane emission on the longwall face and in the goaf. The presented approach considers the operation of a longwall shearer and conveyers and their possible impacts on both direct emissions of methane and migration from adjacent goafs. In this work, an attempt was made to control the advance speed of the virtual mining system based on sample mining data in the longwall 841A area and the abandoned longwall 841B at the Bielszowice Hard Coal Mine. The objective of this study was to verify the suitability of the adopted control algorithm. The results obtained from computer simulations of the mining operation with the developed control algorithm are presented in graphics of methane concentration, shearer advance speed and the speed control system parameters.
Analysis of the Partial Demagnetization Process of Magnets in a Line Start Permanent Magnet Synchronous Motor
Authors: Baranski Mariusz , Szelag Wojciech , Lyskawinski Wieslaw
Published: October 2020

The paper justifies the validity of analyzing the impact of temperature and the process of partial demagnetization of magnets on the operating parameters of machines. To analyze this impact, a field model of coupled electromagnetic and thermal phenomena in a permanent magnet synchronous motor was proposed. The non-linearity of the magnetic circuit, the effect of temperature on the magnetic, electrical and thermal properties of the materials as well as the developed method of modeling the process of partial demagnetization of the magnet were taken into account. Based on this model, an algorithm and software were developed to analyze the effect of temperature and the process of partial demagnetization of magnets on the work of the line start permanent magnet synchronous motor (LSPMSM). The elaborated software was used to study the effect of temperature during the motor starting phase on the magnetization state of the magnets after the start-up process. The calculation results were compared to the results of experimental studies. The experimental tests were carried out on a specially constructed test stand. The results of the research on the process of partial demagnetization of the magnets are presented and the conclusions resulting therefrom formulated.
A Preliminary Assessment of the Potential of Low Percentage Green Hydrogen Blending in the Italian Natural Gas Network
Authors: Pellegrini Marco , Guzzini Alessandro , Saccani Cesare
Published: October 2020

The growing rate of electricity generation from renewables is leading to new operational and management issues on the power grid because the electricity generated exceeds local requirements and the transportation or storage capacities are inadequate. An interesting option that is under investigation by several years is the opportunity to use the renewable electricity surplus to power electrolyzers that split water into its component parts, with the hydrogen being directly injected into natural gas pipelines for both storage and transportation. This innovative approach merges together the concepts of (i) renewable power-to-hydrogen (P2H) and of (ii) hydrogen blending into natural gas networks. The combination of renewable P2H and hydrogen blending into natural gas networks has a huge potential in terms of environmental and social benefits, but it is still facing several barriers that are technological, economic, legislative. In the framework of the new hydrogen strategy for a climate-neutral Europe, Member States should design a roadmap moving towards a hydrogen ecosystem by 2050. The blending of “green hydrogen”, that is hydrogen produced by renewable sources, in the natural gas network at a limited percentage is a key element to enable hydrogen production in a preliminary and transitional phase. Therefore, it is urgent to evaluate at the same time (i) the potential of green hydrogen blending at low percentage (up to 10%) and (ii) the maximum P2H capacity compatible with low percentage blending. The paper aims to preliminary assess the green hydrogen blending potential into the Italian natural gas network as a tool for policy makers, grid and networks managers and energy planners.
Pseudocapacitive Effect of Carbons Doped with Different Functional Groups as Electrode Materials for Electrochemical Capacitors
Authors: Mirzaeian Mojtaba , Abbas Qaisar , Hunt Michael. R. C. , Hall Peter
Published: October 2020

In this study, RF-based un-doped and nitrogen-doped aerogels were produced by polymerisation reaction between resorcinol and formaldehyde with sodium carbonate as catalyst and melamine as the nitrogen source. Carbon/activated carbon aerogels were obtained by carbonisation of the gels under inert atmosphere (Ar) followed by activation of the carbons under CO2 at 800 °C. The BET analysis of the samples showed a more than two-fold increase in the specific Surf. area and pore volume of carbon from 537 to 1333 m2g−1 and 0.242 to 0.671 cm3g−1 respectively after nitrogen doping and activation. SEM and XRD analysis of the samples revealed highly porous amorphous nanostructures with denser inter-particle cross-linked pathways for the activated nitrogen-doped carbon. The X-Ray Photoelectron Spectroscopy (XPS) results confirmed the presence of nitrogen and oxygen heteroatoms on the Surf. and within the carbon matrix where improvement in wettability with the drop in the contact angle from 123° to 80° was witnessed after oxygen and nitrogen doping. A steady drop in the equivalent series (RS) and charge transfer (RCT) resistances was observed by electrochemical measurements after the introduction of nitrogen and oxygen heteroatoms. The highest specific capacitance of 289 Fg−1 with the lowest values of 0.11 Ω and 0.02 Ω for RS and RCT was achieved for nitrogen and oxygen dual-doped activated carbon in line with its improved Surf. chemistry and wettability, and its enhanced conductivity due to denser inter-particle cross-linked pathways.
Adaptive Control of Fuel Cell and Supercapacitor Based Hybrid Electric Vehicles
Authors: Nazir Muhammad Saqib , Ahmad Iftikhar , Khan Muhammad Jawad , Ayaz Yasar , Armghan Hammad
Published: October 2020

In this paper, an adaptive nonlinear control strategy for the energy management of a polymer electrolyte membrane fuel cell and supercapacitor-based hybrid electric vehicle is proposed. The purpose of this work was to satisfy: (i) tight DC bus voltage regulation, (ii) good fuel cell reference current tracking, (iii) better supercapacitor reference current tracking (iv) global asymptotic stability of the closed-loop control system, and (v) better vehicle performance by catering to slowly-varying parameters. We have selected the power stage schematic of a hybrid electric vehicle and utilized adaptive backstepping and adaptive Lyapunov redesign-based nonlinear control methods to formally derive adaptive parametric update laws for all slowly-varying parameters. The performance of the proposed system has been tested under varying load conditions using experimental data from the “Extra Urban Driving Cycle.” Mathematical analysis and Matlab/Simulink results show that proposed controllers are globally asymptotically stable and satisfy all the design requirements. The physical effectiveness of proposed system has been verified by comparing simulation results with the real-time controller hardware in the loop experimental results. Results show that proposed system shows satisfactory performance and caters for the time-varying parametric variations and the load requirements.
A Case Study Balancing Predetermined Targets and Real-World Constraints to Guide Optimum Urban Tree Canopy Cover for Perth, Western Australia
Authors: Parker Jackie , Simpson Greg D.
Published: October 2020

Trees in urban settings are becoming increasingly important as mediators to emerging challenges that transect social, environmental, and economic factors. Trees provide shade; absorb and store atmospheric carbon and other pollutants; reduce local temperature fluctuations; provide essential inner-city fauna habitat; assist in reducing over-land stormwater flow; provide amenity; and provide many more social, environmental, and economic benefits. To secure these benefits, tree canopy cover targets are commonly employed by land managers; however, such targets are rarely quantified against the characteristics and limitations of individual urban centers. Through the generation and interrogation of qualitative and quantitative data, this case study of Perth, Western Australia presents a new conceptual tool that integrates eleven factors found to influence the capacity and opportunity for a city to support urban tree canopy cover. This tool is designed to capture and causally weigh urban tree canopy considerations based on individual city characteristics, collective values, and identifiable constraints. The output of the tool provides an “optimum” tree canopy cover result (as a percentage of the urban fabric) to better inform canopy cover targets and recommendations for urban tree strategic planning and management. This tool is valuable for urban land managers, city planners, urban designers, and communities in effective planning, management, valuation, and investment regarding urban trees as a sub-set of urban green infrastructure.
Snapshot of Cyprus Raw Goat Milk Bacterial Diversity via 16S rDNA High-Throughput Sequencing; Impact of Cold Storage Conditions
Authors: Kamilari Eleni , Anagnostopoulos Dimitrios A. , Papademas Photis , Efthymiou Marina , Tretiak Svitlana , Tsaltas Dimitrios
Published: October 2020

In general, it is a common practice among dairy producers to store the milk in the refrigerator directly after milking, in order to preserve it and prevent the development of spoilage microbes. However, the impact of keeping the milk in the refrigerator overnight on milk microbial diversity has been poorly investigated. This study aimed to provide a snapshot of the bacterial composition of goat milk after direct storage at −80 °C and after being kept overnight at 4 °C and then in storage at −80 °, using high-throughput sequencing (HTS). Goat milk samples from four different farms were analyzed, to reveal that milk bacterial diversity differed between the two different storage conditions. Goat milk directly stored at −80 °C was characterized by the presence of the Gram-negative contaminants Pseudomonas and Acinetobacter, in addition to the genera Corynebacterium, Chryseobacterium, Bacteroides and Clostridium. Milk samples that were kept overnight at 4 °C were characterized by a reduction in their bacterial biodiversity and the predominance of the Gram-negative, aerobic Phyllobacterium. Overall, HTS methodologies provide an in-depth identification and characterization of the goat raw milk microbiome. Further, they offer a better understanding of the contribution of cold storage conditions to milk microbiota formation. This study may assist dairy producers in improving raw milk and raw milk cheeses quality and guaranteeing consumers’ safety.
SSR-Marker Analysis—A Method for S. cerevisiae Strain Characterization and Its Application for Wineries
Authors: Rex Friederike , Hirschler Adeline , Scharfenberger-Schmeer Maren
Published: October 2020

Considering that many Saccharomyces cerevisiae strains exist and that they have different fermentation capacities, the challenge is to select the yeast strain that generates the most interesting wine character and wine flavor for the winemaker. A method based on simple sequence repeats (SSRs) markers, occurring in the yeast genome, was developed to differentiate the collected S.cerevisiae strains. For the amplification of the polymorphic SSR markers performed by polymerase chain reaction (PCR), two primer sets showing different size products for different S. cerevisiae strains were designed. The PCR-method with gel electrophoresis was validated using capillary sequencing and then used as a service for winegrowers combined with a sensory analysis via napping. This approach can be used for the preservation of the yeast diversity associated with given terroirs and as an option for an increased safety of fermentations. The application of S. cerevisiae strains collected in spontaneous fermentations and used for fermentation sustains the initial character of the wine and ensures a secure fermentation at the same time.
Uncovering Fine-Scale Wave-Driven Transport Features in a Fringing Coral Reef System via Lagrangian Coherent Structures
Authors: Leclair Matthieu , Lowe Ryan , Zhang Zhenlin , Ivey Greg , Peacock Thomas
Published: October 2020

Understanding the transport and exchange of water masses both within a reef and between a reef and the surrounding ocean is needed to describe a wide-range of ecosystem processes that are shaped by the movement of material and heat. We show how novel Lagrangian data processing methods, specifically developed to reveal key and often hidden transport structures, can help visualize flow transport patterns within and around morphologically complex reef systems. As an example case study, we consider the wave-driven flow transport within the Ningaloo Reef in Western Australia. We show that a network of attracting, repelling, and trapping flow transport structures organizes the flow transport into, around, and out of the reef. This approach is broadly applicable to coral reef systems, since the combination of well-defined bathymetry and persistent flow-forcing mechanisms (e.g., by wave breaking or tides) is conducive to the existence of persistent Lagrangian transport structures that organize material transport.
Analysis of Association between the Consumer Food Quality Perception and Acceptance of Enhanced Meat Products and Novel Packaging in a Population-Based Sample of Polish Consumers
Authors: Guzek Dominika , Głąbska Dominika , Sajdakowska Marta , Gutkowska Krystyna
Published: October 2020

The consumer acceptance of novel enhanced-quality products and their willingness to buy such products may be a crucial topic in the field of marketing. The aim of this study was to analyze the association between consumers’ perceptions of food quality and their acceptance of enhanced meat products and novel packaging. The study was conducted using the Computer-Assisted Personal Interview (CAPI) method in a random group of 1009 respondents, who were recruited as a representative sample based on data from the Polish National Identification Number database. The participants were asked about the most important quality determinants of food products of animal origin and about quality improvement methods and their acceptance of those methods. The quality determinants of animal-based food products were indicated as follows: origin, production technology, manufacturer, components and nutritional value, visual and sensory characteristics, expiry date, and cost. The quality improvement methods were clustered into groups that were associated with product enhancement and application of novel packaging, and the acceptance of those methods was also verified. Indicating specific quality determinants of animal-derived food products affects the consumer acceptance of product enhancement (p = 0.0264) and novel packaging as quality improvement methods (p = 0.0314). The understanding that enhancement is applied for the purpose of quality improvement did not influence the acceptance of products (p = 0.1582), whereas the knowledge that novel packaging is applied influenced the acceptance (p = 0.0044). The obtained results suggested that in the case of application of novel packaging, a higher level of knowledge may be a reason for consumer’s rejection of the resulting products, but the appearance and taste of products may contribute to the higher acceptance of novel packaging. Educating consumers may improve their acceptance of product enhancement, as concerns about the addition of food preservatives may lead them to reject enhanced products.
Control of Biological Hazards in Insect Processing: Application of HACCP Method for Yellow Mealworm (Tenebrio molitor) Powders
Authors: Kooh Pauline , Jury Vanessa , Laurent Sophie , Audiat-Perrin Frédérique , Sanaa Moez , Tesson Vincent , Federighi Michel , Boué Géraldine
Published: October 2020

Entomophagy has been part of human diets for a long time in a significant part of the world, but insects are considered to be a novel food everywhere else. It would appear to be a strategic alternative in the future of human diet to face the challenge of ensuring food security for a growing world population, using more environmentally sustainable production systems than those required for the rearing of other animals. Tenebrio molitor, called yellow mealworm, is one of the most interesting insect species in view of mass rearing, and can be processed into a powder that ensures a long shelf life for its use in many potential products. When considering insects as food or feed, it is necessary to guarantee their safety. Therefore, manufacturers must implement a Hazard Analysis Critical Control plan (HACCP), to limit risks for consumers’ health. The aim of this case study was to develop a HACCP plan for Tenebrio molitor larvae powders for food in a risk-based approach to support their implementation in industry. Specific purposes were to identify related significant biological hazards and to assess the efficiency of different manufacturing process steps when used as Critical Control Points. Then, combinations of four different processes with four potential uses of powders by consumers in burger, protein shake, baby porridge, and biscuits were analyzed with regard to their safety.
Moment of Bentonite Addition, Co-Addition of Tannins, and Bentonite Type Affect the Differential Affinity of Pathogenesis-Related Grape Proteins towards Bentonite during Fermentation
Authors: Lukić Igor , Horvat Ivana
Published: October 2020

To test the effect of the moment of bentonite addition, co-addition of tannins, and bentonite type on the differential affinity of pathogenesis-related (PR) proteins towards bentonite during grape must fermentation, three separate experiments were set up. PR proteins in the obtained wines were analyzed by reverse phase and size exclusion high-performance liquid chromatography (HPLC). The most significant reduction of bentonite dose and PR protein concentration was achieved by applying bentonite in the last third of fermentation. Particular thaumatin-like proteins (TLP) and proteins with lower molecular mass in general were more affected than others, while TLPs were more affected than chitinases. Exogenous enological tannins interacted with particular PR proteins, mostly TLPs, and lowered the total bentonite dose required. The combined application of tannins and bentonite in fermentation removed more PR proteins than bentonite alone, but did not achieve a synergistic effect in reducing the bentonite dose. Various bentonite types, including two Na-activated bentonites, an activated Na bentonite with specifically adsorbed silica, and an active Na-Ca bentonite, exhibited differential affinity towards different PR proteins. The results obtained could be used in developing wine fining protocols which combine treatments with complementary affinity for adsorption and removal of PR proteins, and in this way achieve greater efficiency of bentonite fining by reducing its total dose, which is of significant interest to the wine industry.
Sous-Vide as a Technique for Preparing Healthy and High-Quality Vegetable and Seafood Products
Authors: Zavadlav Sandra , Blažić Marijana , Van de Velde Franco , Vignatti Charito , Fenoglio Cecilia , Piagentini Andrea M. , Pirovani María Elida , Perotti Cristina M. , Bursać Kovačević Danijela , Putnik Predrag
Published: October 2020

Sous-vide is a technique of cooking foods in vacuum bags under strictly controlled temperature, offering improved taste, texture and nutritional values along with extended shelf life as compared to the traditional cooking methods. In addition to other constituents, vegetables and seafood represent important sources of phytochemicals. Thus, by applying sous-vide technology, preservation of such foods can be prolonged with almost full retention of native quality. In this way, sous-vide processing meets customers’ growing demand for the production of safer and healthier foods. Considering the industrial points of view, sous-vide technology has proven to be an adequate substitute for traditional cooking methods. Therefore, its application in various aspects of food production has been increasingly researched. Although sous-vide cooking of meats and vegetables is well explored, the challenges remain with seafoods due to the large differences in structure and quality of marine organisms. Cephalopods (e.g., squid, octopus, etc.) are of particular interest, as the changes of their muscular physical structure during processing have to be carefully considered. Based on all the above, this study summarizes the literature review on the recent sous-vide application on vegetable and seafood products in view of production of high-quality and safe foodstuffs.