bluuebunny/crossref_metadata_2025 huggingface dataset:
Climate-Driven Holocene Migration of Forest-Steppe Ecotone in the Tien Mountains
Authors: Cheng Ying , Liu Hongyan , Wang Hongya , Hao Qian , Han Yue , Duan Keqin , Dong Zhibao
Published: October 2020

Climate change poses a considerable threat to the forest-steppe ecotone in arid mountain areas. However, it remains unclear how the forest-steppe ecotone responds to climate change due to the limitation of the traditional pollen assemblages, which greatly limits the understanding of the history of the forest-steppe ecotone. Here, we examined the Tien Mountains, the largest mountain system in the world’s arid regions, as a case study to explore the migration of the forest-steppe ecotone using the pollen taxa diversity, by combining modern vegetation surveys, surface pollen and two fossil pollen sequences—in the mid-elevation forest belt (Sayram Lake) and in the low-elevation desert belt (Aibi Lake). We found that the forest-steppe migration followed Holocene climate change. Specifically, the forest belt where Picea schrenkiana Fisch. & C.A.Mey. dominates has a very low pollen taxa diversity, characterized by high richness and low evenness, which plays a key role in mountainous diversity. By detecting the diversity change of the deposition sites, we found that in coping with the warm and wet middle Holocene, the forest belt expanded and widened as the observed diversities around the two lakes were very low, thus the forest-steppe ecotone moved downward accordingly. During the early and late Holocene, the forest belt and the forest-steppe ecotone moved upward under a warm and dry climate, and downward under a cold and wet climate, as there was a reduced forest belt effect on, or contribution to, the sites, and the observed diversities were high. Moisture loss may pose the greatest threat to the narrow forest-steppe ecotone, considering the climatic niche space and the limited living space for humidity-sensitive taxa. This study highlights that temperature and moisture co-influence the forest belt change, which further determines the position migration of the forest-steppe ecotone.
Tetraethyl Orthosilicate-Based Hydrogels for Drug Delivery—Effects of Their Nanoparticulate Structure on Release Properties
Authors: Serban Bogdan A. , Barrett-Catton Emma , Serban Monica A.
Published: October 2020

Tetraethyl orthosilicate (TEOS)-based hydrogels, with shear stress response and drug releasing properties, can be formulated simply by TEOS hydrolysis followed by volume corrections with aqueous solvents and pH adjustments. Such basic thixotropic hydrogels (thixogels) form via the colloidal aggregation of nanoparticulate silica. Herein, we investigated the effects of the nanoparticulate building blocks on the drug release properties of these materials. Our data indicate that the age of the hydrolyzed TEOS used for the formulation impacts the nanoparticulate structure and stiffness of thixogels. Moreover, the mechanism of formation or the disturbance of the nanoparticulate network significantly affects the release profiles of the incorporated drug. Collectively, our results underline the versatility of these basic, TEOS-only hydrogels for drug delivery applications.
Non-Invasive Study on the Sinope Gospels
Authors: Aceto Maurizio , Calà Elisa , Agostino Angelo , Fenoglio Gaia , Labate Maria , Förstel Christian , Denoël Charlotte , Quandt Abigail
Published: October 2020

The 6th century Codex Sinopensis or Sinope Gospels (Paris, Bibliothèque nationale de France) is one of the most precious purple codices that survive from the Late Antique period. Together with the Vienna Genesis (Wien, Österreichische Nationalbibliothek) and the Rossano Gospels (Rossano Calabro, Museo Diocesano), it has an unusually rich decorative apparatus with scenes representing biblical episodes. It can be, therefore, considered one of the most important preserved artistic productions of the early medieval era. The manuscript has been subjected to a non-invasive diagnostic campaign to evaluate the quality of the colourants used in its decoration, to understand how the parchment was coloured, and to carry out a comparison with the Vienna Genesis and the Rossano Gospels. The techniques used were UV-visible diffuse reflectance spectrophotometry with optical fibres (FORS), X-ray fluorescence spectrometry (XRF), spectrofluorimetry, and optical microscopy. Analyses highlighted the presence of ultramarine blue, which, besides the use of pure gold for the ink and paint, certifies the high value of the manuscript. In addition, this must be seen as one of the earliest examples of its use in paintings. The purple colour of the parchment was identified as orchil, a dye extracted from lichens, similar to the results of analytical investigations carried out on other purple codices, and not the expected Tyrian purple dye.
Parental Attitudes to Childhood Overweight: The Multiple Paths through Healthy Eating, Screen Use, and Sleeping Time
Authors: Giménez Garcia-Conde Miguel , Marin Longinos , Ruiz de Maya Salvador , Cuestas Pedro J.
Published: October 2020

The main goal of this paper is to examine how parental attitudes toward sleeping, screen use, and feeding their child influence the child’s body mass index (BMI) through the child’s behaviors related to time dedicated to sleep and television, and their healthy eating. Using survey data from 908 parents, results show that parental attitudes have a significant influence on children’s behavior and, more importantly, on their BMI. The three forms of a child’s behavior (sleeping, watching TV, and healthy eating patterns) are affected by what parents do and their attitudes toward these three behaviors at home. Results show how changes in parental attitudes are an interesting target to prevent the child’s overweight.
Association of eHealth Use, Literacy, Informational Social Support, and Health-Promoting Behaviors: Mediation of Health Self-Efficacy
Authors: Choi MoonKi
Published: October 2020

This descriptive, cross-sectional study identified the association of eHealth use, literacy, informational support, and health-promoting behaviors among older adults, as mediated by health self-efficacy. Convenience sampling was conducted at senior welfare centers in Chuncheon, in the Republic of Korea. Data analysis was performed using Pearson’s correlation and via path analyses. The findings showed that eHealth use had an indirect effect on health-promoting behaviors, as mediated by self-efficacy. Informational support was indirectly mediated by self-efficacy and had direct effects upon health-promoting behaviors. eHealth can facilitate self-efficacy and health management, despite not having direct effects upon health-promoting behaviors themselves. Thus, older adults need to be prepared for the increased use of eHealth. In addition, healthcare professionals should support older people in their use of eHealth and encourage informational support through comprehensive interventions so as to facilitate self-efficacy and health behaviors.
Silver Nanostars-Coated Surfaces with Potent Biocidal Properties
Authors: Bessa Lucinda J. , Peixoto de Almeida Miguel , Eaton Peter , Pereira Eulália , Gameiro Paula
Published: October 2020

Bacterial proliferation on certain surfaces is of concern as it tends to lead to infectious health problems. Nanotechnology is offering new options for engineering antimicrobial surfaces. Herein, the antibiofilm and biocidal properties of star-shaped silver nanoparticles (AgNSs) in suspension and as coating surfaces were studied. AgNSs and spherical silver nanoparticles (AgNPs) (used for comparison purposes) were synthesized using reported methods. Glass disks (9 mm diameter) were covered with AgNSs using deposition by centrifugation. Minimum inhibitory concentrations (MICs) of AgNSs and AgNPs were determined against several reference strains and multidrug-resistant isolates and their antibiofilm activity was assessed against preformed biofilms of Pseudomonas aeruginosa and Staphylococcus aureus by both Live/Dead staining and atomic force microscopy (AFM). The antimicrobial properties of AgNSs-coated surfaces were evaluated by the “touch test” method on agar, and also Live/Dead staining and AFM. The MIC values of the AgNSs were 2–4 times lower than those of the AgNPs. Biofilms treated with AgNSs at a concentration equal to the MIC were not significantly affected, although they exhibited more dead cells than the non-treated biofilms. The biocidal activity of AgNSs-coated surfaces was attested, since no growth on agar nor viable cells were observed after contact of the inoculated bacteria with the coated surface for 6 and 24 h. Thus, AgNSs show greater potential as a surface coating with biocidal effects than used as suspension for antimicrobial purposes.
Associations between Child Snack and Beverage Consumption, Severe Dental Caries, and Malnutrition in Nepal
Authors: Zahid Neha , Khadka Nehaa , Ganguly Madhurima , Varimezova Tanya , Turton Bathsheba , Spero Laura , Sokal-Gutierrez Karen
Published: October 2020

The global nutrition transition and increased consumption of sugar-sweetened beverages and ultra-processed snacks have contributed to increasing rates of child obesity and dental caries in developing countries. In Nepal, where child malnutrition rates are high, the relationship between malnutrition and dental caries is poorly understood. This cross-sectional study aims to assess this relationship among a convenience sample of 273 children age six months to less than 12 years in three communities in Nepal, using parent/caregiver interviews, child dental exams, and anthropometric measurements. Fisher’s exact test and independent t-tests examined associations between dietary practices and severe caries and between severe caries and malnutrition, respectively. Children consumed sugar-sweetened beverages and processed snacks frequently: 80% consumed tea with sugar, 60% consumed sweet snacks, and 65% consumed processed savory snacks daily. Overall, 74% of children had untreated tooth decay, and 21% exhibited stunting malnutrition, 14% were underweight, and 6% presented wasting. Significant associations were found between daily consumption of sweets and processed snacks with severe caries and between severe caries and poorer nutritional status. These findings underscore the need to incorporate nutrition and oral health promotion and dental treatment into maternal–child health services and schools and to strengthen policies to reduce children’s access to junk food.
Fresh Agricultural Products Supply Chain Coordination and Volume Loss Reduction Based on Strategic Consumer
Authors: Qiu Fang , Hu Qifan , Xu Bing
Published: October 2020

The reduction of fresh agricultural product volume loss throughout the supply chain system is of high importance due to their perishable nature and impact on society, the economy, and environment. In this paper, three models for two-stage pricing, coordination, and volume loss reduction of the supply chain where third-party logistics service providers and retailers act as a Stackelberg leader and a follower for fresh agricultural products are developed, taking into account both volume loss during transport and quality loss in retail in the presence of strategic consumers. The following results are drawn from the contract for sharing revenues and service costs: (1) The supply chain achieve coordination and the products are healthier for consumers; (2) the coordination leads to a reduction in the three types of volume losses simultaneously only if the lowest marginal costs of the supply chain occur under certain conditions; and (3) the increase in the service sensitivity coefficient, the increase in the freshness discount coefficient under certain conditions, the decrease in the consumer benefit discount coefficient under certain conditions, and the decrease in the price sensitivity coefficient lead to an increase in the profit of the supply chain and a reduction in the three types of volume losses.
Socioecological Predictors on Psychological Flourishing in the US Adolescence
Authors: Kim TaeEung , Jang Chang-Yong , Kim Minju
Published: October 2020

This study examined the relationship between children’s flourishing and socioecological factors, including individual and family characteristics. A total of 45,309 children were drawn from the 2011–2012 National Survey of Children’s Health in the US (mean age = 13.6 years; male: 51.28%). An ordinary least square (OLS) regression was performed to examine the association between socioecological variables and flourishing. This study assessed children’s individual needs (such as health, education, and emotional and behavioral development), their parents’ parenting capacity (the ability to provide basic care and safety), and family factors (presence of community resources and family income). Children’s individual characteristics, parent’s capacities, and family functions were found to be significantly associated with children’s flourishing. In conclusion, multilevel socioecological factors appeared to be associated with children’s flourishing. Hence, parents’ involvement in their children’s physical activities, as well as family and social support, is crucial for children’s flourishing. This study makes a significant contribution to the literature as research is lacking a correlation between socioecological factors and children’s flourishing; in particular, very few studies have explored or investigated the manner by which children’s socioecological health indicators and factors are associated with their flourishing.
Beyond Conventional: The New Horizon of Anti-Angiogenic microRNAs in Non-Small Cell Lung Cancer Therapy
Authors: Tirpe Alexandru , Gulei Diana , Tirpe George Razvan , Nutu Andreea , Irimie Alexandru , Campomenosi Paola , Pop Laura Ancuta , Berindan-Neagoe Ioana
Published: October 2020

GLOBOCAN 2018 identified lung cancer as the leading oncological pathology in terms of incidence and mortality rates. Angiogenesis is a key adaptive mechanism of numerous malignancies that promotes metastatic spread in view of the dependency of cancer cells on nutrients and oxygen, favoring invasion. Limitation of the angiogenic process could significantly hamper the disease advancement through starvation of the primary tumor and impairment of metastatic spread. This review explores the basic molecular mechanisms of non-small cell lung cancer (NSCLC) angiogenesis, and discusses the influences of the key proangiogenic factors—the vascular endothelial growth factor-A (VEGF-A), basic fibroblast growth factor (FGF2), several matrix metalloproteinases (MMPs—MMP-2, MMP-7, MMP-9) and hypoxia—and the therapeutic implications of microRNAs (miRNAs, miRs) throughout the entire process, while also providing critical reviews of a number of microRNAs, with a focus on miR-126, miR-182, miR-155, miR-21 and let-7b. Finally, current conventional NSCLC anti-angiogenics—bevacizumab, ramucirumab and nintedanib—are briefly summarized through the lens of evidence-based medicine.
Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer’s Disease
Authors: Dhakal Sudip , Macreadie Ian
Published: October 2020

Alzheimer’s Disease (AD) is a progressive multifactorial age-related neurodegenerative disorder that causes the majority of deaths due to dementia in the elderly. Although various risk factors have been found to be associated with AD progression, the cause of the disease is still unresolved. The loss of proteostasis is one of the major causes of AD: it is evident by aggregation of misfolded proteins, lipid homeostasis disruption, accumulation of autophagic vesicles, and oxidative damage during the disease progression. Different models have been developed to study AD, one of which is a yeast model. Yeasts are simple unicellular eukaryotic cells that have provided great insights into human cell biology. Various yeast models, including unmodified and genetically modified yeasts, have been established for studying AD and have provided significant amount of information on AD pathology and potential interventions. The conservation of various human biological processes, including signal transduction, energy metabolism, protein homeostasis, stress responses, oxidative phosphorylation, vesicle trafficking, apoptosis, endocytosis, and ageing, renders yeast a fascinating, powerful model for AD. In addition, the easy manipulation of the yeast genome and availability of methods to evaluate yeast cells rapidly in high throughput technological platforms strengthen the rationale of using yeast as a model. This review focuses on the description of the proteostasis network in yeast and its comparison with the human proteostasis network. It further elaborates on the AD-associated proteostasis failure and applications of the yeast proteostasis network to understand AD pathology and its potential to guide interventions against AD.
Blue Light Mediates Chloroplast Avoidance and Enhances Photoprotection of Vanilla Orchid
Authors: Ko Swee-Suak , Jhong Chung-Min , Lin Yi-Jyun , Wei Ching-Yu , Lee Ju-Yin , Shih Ming-Che
Published: October 2020

Vanilla orchid, which is well-known for its flavor and fragrance, is cultivated in tropical and subtropical regions. This shade-loving plant is very sensitive to high irradiance. In this study, we show that vanilla chloroplasts started to have avoidance movement when blue light (BL) was higher than 20 μmol m−2s−1 and significant avoidance movement was observed under BL irradiation at 100 μmol m−2s−1 (BL100). The light response curve indicated that when vanilla was exposed to 1000 μmol m−2s−1, the electron transport rate (ETR) and photochemical quenching of fluorescence (qP) were significantly reduced to a negligible amount. We found that if a vanilla orchid was irradiated with BL100 for 12 days, it acquired BL-acclimation. Chloroplasts moved to the side of cells in order to reduce light-harvesting antenna size, and chloroplast photodamage was eliminated. Therefore, BL-acclimation enhanced vanilla orchid growth and tolerance to moderate (500 μmol m−2s−1) and high light (1000 μmol m−2s−1) stress conditions. It was found that under high irradiation, BL-acclimatized vanilla maintained higher ETR and qP capacity than the control without BL-acclimation. BL-acclimation induced antioxidant enzyme activities, reduced ROS accumulation, and accumulated more carbohydrates. Moreover, BL-acclimatized orchids upregulated photosystem-II-associated marker genes (D1 and PetC), Rubisco and PEPC transcripts and sustained expression levels thereof, and also maximized the photosynthesis rate. Consequently, BL-acclimatized orchids had higher biomass. In short, this study found that acclimating vanilla orchid with BL before transplantation to the field might eliminate photoinhibition and enhance vanilla growth and production.
Dead but Not Dead End: Multifunctional Role of Dead Organs Enclosing Embryos in Seed Biology
Authors: Grafi Gideon
Published: October 2020

Dry fruits consist of two types, dehiscent and indehiscent, whereby the fruit is splitting open or remains closed at maturity, respectively. The seed, the dispersal unit (DU) of dehiscent fruits, is composed of three major parts, the embryo and the food reserve, encapsulated by the maternally-derived organ, the seed coat. Indehiscent fruit constitutes the DU in which the embryo is covered by two protective layers (PLs), the seed coat and the fruit coat. In grasses, the caryopsis, a one-seeded fruit, can be further enclosed by the floral bracts to generate two types of DUs, florets and spikelets. All protective layers enclosing the embryo undergo programmed cell death (PCD) at maturation and are thought to provide mainly a physical shield for embryo protection and a means for dispersal. In this review article, I wish to highlight the elaborate function of these dead organs enclosing the embryo as unique storage structures for beneficial substances and discuss their potential role in seed biology and ecology.
Radiotherapy-Resistant Breast Cancer Cells Enhance Tumor Progression by Enhancing Premetastatic Niche Formation through the HIF-1α-LOX Axis
Authors: Ko Young Shin , Rugira Trojan , Jin Hana , Joo Young Nak , Kim Hye Jung
Published: October 2020

Cancer stem cells (CSCs) exist in solid tumors and contribute to therapeutic resistance and disease recurrence. Previously, we reported that radiotherapy-resistant (RT-R)-MDA-MB-231 cells from highly metastatic MDA-MB-231 cells produced more CSCs than any other RT-R-breast cancer cells and showed therapeutic resistance and enhanced invasiveness. Hypoxia inducible factor-1α (HIF-1α) induced in the tumor microenvironment leads to the release of lysyl oxidase (LOX), which mediates collagen crosslinking at distant sites to facilitate environmental changes that allow cancer cells to easily metastasize. Therefore, in this study, we investigated whether RT-R-MDA-MB-231 cells induce greater HIF-1α expression, LOX secretion, and premetastatic niche formation than MDA-MB-231 cells do. RT-R-MDA-MB-231 cells increased HIF-1α expression and LOX secretion compared with MDA-MB-231 cells. Mice harboring RT-R-MDA-MB-231 cell xenografts showed enhanced tumor growth and higher expression of the CSC markers, CD44, Notch-4, and Oct3/4. In addition, mice injected with RT-R-MDA-MB-231 cells exhibited a higher level of HIF-1α in tumor tissue, increased secretion of LOX in plasma, higher induced levels of crosslinked collagen, and a higher population of CD11b+ BMDC recruitment around lung tissue, compared with those injected with MDA-MB-231 cells. These results suggest that RT-R-MDA-MB-231 cells contribute to tumor progression by enhancing premetastatic niche formation through the HIF-1α-LOX axis.
Osmosis-Driven Water Transport through a Nanochannel: A Molecular Dynamics Simulation Study
Authors: Eun Changsun
Published: October 2020

In this work, we study a chemical method to transfer water molecules from a nanoscale compartment to another initially empty compartment through a nanochannel. Without any external force, water molecules do not spontaneously move to the empty compartment because of the energy barrier for breaking water hydrogen bonds in the transport process and the attraction between water molecules and the compartment walls. To overcome the energy barrier, we put osmolytes into the empty compartment, and to remove the attraction, we weaken the compartment-water interaction. This allows water molecules to spontaneously move to the empty compartment. We find that the initiation and time-transient behavior of water transport depend on the properties of the osmolytes specified by their number and the strength of their interaction with water. Interestingly, when osmolytes strongly interact with water molecules, transport immediately starts and continues until all water molecules are transferred to the initially empty compartment. However, when the osmolyte interaction strength is intermediate, transport initiates stochastically, depending on the number of osmolytes. Surprisingly, because of strong water-water interactions, osmosis-driven water transport through a nanochannel is similar to pulling a string at a constant speed. Our study helps us understand what minimal conditions are needed for complete transfer of water molecules to another compartment through a nanochannel, which may be of general concern in many fields involving molecular transfer.
Cardiolipin, the Mitochondrial Signature Lipid: Implication in Cancer
Authors: Ahmadpour Seyedeh Tayebeh , Mahéo Karine , Servais Stéphane , Brisson Lucie , Dumas Jean-François
Published: October 2020

Cardiolipins (CLs) are specific phospholipids of the mitochondria composing about 20% of the inner mitochondria membrane (IMM) phospholipid mass. Dysregulation of CL metabolism has been observed in several types of cancer. In most cases, the evidence for a role for CL in cancer is merely correlative, suggestive, ambiguous, and cancer-type dependent. In addition, CLs could play a pivotal role in several mitochondrial functions/parameters such as bioenergetics, dynamics, mitophagy, and apoptosis, which are involved in key steps of cancer aggressiveness (i.e., migration/invasion and resistance to treatment). Therefore, this review focuses on studies suggesting that changes in CL content and/or composition, as well as CL metabolism enzyme levels, may be linked with the progression and the aggressiveness of some types of cancer. Finally, we also introduce the main mitochondrial function in which CL could play a pivotal role with a special focus on its implication in cancer development and therapy.
Interaction between Trinuclear Regium Complexes of Pyrazolate and Anions, a Computational Study
Authors: Alkorta Ibon , Elguero José , Trujillo Cristina , Sánchez-Sanz Goar
Published: October 2020

The geometry, energy and electron density properties of the 1:1, 1:2 and 1:3 complexes between cyclic (Py-M)3 (M = Au, Ag and Cu) and halide ions (F−, Cl− and Br−) were studied using Møller Plesset (MP2) computational methods. Three different configurations were explored. In two of them, the anions interact with the metal atoms in planar and apical dispositions, while in the last configuration, the anions interact with the CH(4) group of the pyrazole. The energetic results for the 1:2 and 1:3 complexes are a combination of the specific strength of the interaction plus a repulsive component due to the charge:charge coulombic term. However, stable minima structures with dissociation barriers for the anions indicate that those complexes are stable and (Py-M)3 can hold up to three anions simultaneously. A search in the CSD confirmed the presence of (Pyrazole-Cu)3 systems with two anions interacting in apical disposition.
Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division
Authors: Honsho Masanori , Abe Yuichi , Imoto Yuuta , Chang Zee-Fen , Mandel Hanna , Falik-Zaccai Tzipora C. , Fujiki Yukio
Published: October 2020

Peroxisomes proliferate by sequential processes comprising elongation, constriction, and scission of peroxisomal membrane. It is known that the constriction step is mediated by a GTPase named dynamin-like protein 1 (DLP1) upon efficient loading of GTP. However, mechanism of fuelling GTP to DLP1 remains unknown in mammals. We earlier show that nucleoside diphosphate (NDP) kinase-like protein, termed dynamin-based ring motive-force organizer 1 (DYNAMO1), generates GTP for DLP1 in a red alga, Cyanidioschyzon merolae. In the present study, we identified that nucleoside diphosphate kinase 3 (NME3), a mammalian homologue of DYNAMO1, localizes to peroxisomes. Elongated peroxisomes were observed in cells with suppressed expression of NME3 and fibroblasts from a patient lacking NME3 due to the homozygous mutation at the initiation codon of NME3. Peroxisomes proliferated by elevation of NME3 upon silencing the expression of ATPase family AAA domain containing 1, ATAD1. In the wild-type cells expressing catalytically-inactive NME3, peroxisomes were elongated. These results suggest that NME3 plays an important role in peroxisome division in a manner dependent on its NDP kinase activity. Moreover, the impairment of peroxisome division reduces the level of ether-linked glycerophospholipids, ethanolamine plasmalogens, implying the physiological importance of regulation of peroxisome morphology.
Prototype to Increase Crosswalk Safety by Integrating Computer Vision with ITS-G5 Technologies
Authors: Gaspar Francisco , Guerreiro Vitor , Loureiro Paulo , Costa Paulo , Mendes Sílvio , Rabadão Carlos
Published: October 2020

Human errors are probably the main cause of car accidents, and this type of vehicle is one of the most dangerous forms of transport for people. The danger comes from the fact that on public roads there are simultaneously different types of actors (drivers, pedestrians or cyclists) and many objects that change their position over time, making difficult to predict their immediate movements. The intelligent transport system (ITS-G5) standard specifies the European communication technologies and protocols to assist public road users, providing them with relevant information. The scientific community is developing ITS-G5 applications for various purposes, among which is the increasing of pedestrian safety. This paper describes the developed work to implement an ITS-G5 prototype that aims at the increasing of pedestrian and driver safety in the vicinity of a pedestrian crosswalk by sending ITS-G5 decentralized environmental notification messages (DENM) to the vehicles. These messages are analyzed, and if they are relevant, they are presented to the driver through a car’s onboard infotainment system. This alert allows the driver to take safety precautions to prevent accidents. The implemented prototype was tested in a controlled environment pedestrian crosswalk. The results showed the capacity of the prototype for detecting pedestrians, suitable message sending, the reception and processing on a vehicle onboard unit (OBU) module and its presentation on the car onboard infotainment system.
Energy-Efficient Check-and-Spray Geocast Routing Protocol for Opportunistic Networks
Authors: Khalid Khuram , Woungang Isaac , Dhurandher Sanjay Kumar , Singh Jagdeep , J. P. C. Rodrigues Joel
Published: October 2020

Opportunistic networks (OppNets) are a type of challenged network where there is no guaranteed of end-to-path between the nodes for data delivery because of intermittent connectivity, node mobility and frequent topology changes. In such an environment, the routing of data is a challenge since the battery power of the mobile nodes drains out quickly because of multi-routing activities such as scanning, transmitting, receiving, and computational processing, effecting the overall network performance. In this paper, a novel routing protocol for OppNets called Energy-Efficient Check-and-Spray Geocast Routing (EECSG) is proposed, which introduces an effective way of message distribution in the geocasting region to all residing nodes while saving the energy consumption by restricting the unnecessary packet transmission in that region. A Check-and-Spray technique is also introduced to eliminate the overhead of packets in the geocast region. The proposed EECSG is evaluated by simulations and compared against the Efficient and Flexible Geocasting for Opportunistic Networks (GSAF) and the Centrality- Based Geocasting for Opportunistic networks (CGOPP) routing protocols in terms of average latency, delivery ratio, number of messages forwarded, number of dead nodes, overhead ratio, and hop count, showing superior performance.
Miniaturized On-Chip NFC Antenna versus Screen-Printed Antenna for the Flexible Disposable Sensor Strips
Authors: Kordzadeh Atefeh , Holzmann Dominik , Binder Alfred , Moldaschl Thomas , Sturm Johannes , Roshanghias Ali
Published: October 2020

With the ongoing trend toward miniaturization via system-on-chip (SoC), both radio-frequency (RF) SoCs and on-chip multi-sensory systems are gaining significance. This paper compares the inductance of a miniaturized on-chip near field communication (NFC) antenna versus the conventional screen-printed on-substrate ones that have been used for the transfer of sensory data from a chip to a cell phone reader. Furthermore, the transferred power efficiency in a coupled NFC system is calculated for various chip coil geometries and the results are compared. The proposed NFC antenna was fabricated via a lithography process for an application-specific integrated circuit (ASIC) chip. The chip had a small area of 2.4 × 2.4 mm2, therefore a miniaturized NFC antenna was designed, whereas the screen-printed on-substrate antennas had an area of 35 × 51 mm2. This paper investigates the effects of different parameters such as conductor thickness and materials, double layering, and employing ferrite layers with different thicknesses on the performance of the on-chip antennas using full-wave simulations. The presence of a ferrite layer to increase the inductance of the antenna and mitigate the interactions with backplates has proven useful. The best performance was obtained via double-layering of the coils, which was similar to on-substrate antennas, while a size reduction of 99.68% was gained. Consequently, the coupling factors and maximum achievable power transmission efficiency of the on-chip antenna and on-substrate antenna were studied and compared.
A 100 MHz 0.41 fJ/(Bit∙Search) 28 nm CMOS-Bulk Content Addressable Memory for HEP Experiments
Authors: Fary Federico , Baschirotto Andrea
Published: October 2020

This paper presents a transistor-level design with extensive experimental validation of a Content Addressable Memory (CAM), based on an eXclusive OR (XOR) single-bit cell. This design exploits a dedicated architecture and a fully custom approach (both in the schematic and the layout phase), in order to achieve very low-power and high-speed performances. The proposed architecture does not require an internal clock or pre-charge phase, which usually increase the power request and slow down data searches. On the other hand, the dedicated solutions are exploited in order to minimize parasitic layout-induced capacitances in the single-bit cell, further reducing the power consumption. The prototype device, named CAM-28CB, is integrated in the deeply downscaled 28 nm Complementary Metal-Oxide-Semiconductor (CMOS) Bulk (28CB) technology. In this way, the high transistor transition frequency and the intrinsic lower parasitic capacitances allow the system speed to be improved. Furthermore, the high radiation hardness of this technology node (up to 1Grad TID), together with the CAM-28CB high-speed and low-power performances, makes this device suitable for High-Energy Physics experiments, such as ATLAS (A Toroidal LHC ApparatuS) at Large Hadron Collider (LHC). The prototype operates at a frequency of up to 100 MHz and consumes 46.86 µW. The total area occupancy is 1702 µm2 for 1.152 kb memory bit cells. The device operates with a single supply voltage of 1 V and achieves 0.41 fJ/bit/search Figure-of-Merit.
Dysregulation of Endothelin-1: Implications for Health Disparities in Alzheimer’s Disease
Authors: Alcendor Donald J.
Published: October 2020

Alzheimer’s disease (AD) and related dementias disproportionately impact racial and ethnic minorities. The racial and ethnic disparities in AD could be explained by differences in cerebral vascular disease pathology. Endothelin-1 (ET-1) is a potent vasoconstrictive peptide that regulates smooth muscle, endothelial cell, and pericyte contractions that may result in cerebral vascular constriction, leading to cerebral hypoperfusion; over time, ET-1 may result in neuronal injury contributing to the pathology of AD. Upregulation of the ET-1 system has been observed in African Americans when compared with non-Hispanic Whites. The role of the ET-1 system as a driver of ethnic disparities in AD requires further investigation. Targeting of the ET-1 system as a therapeutic intervention that could impact AD progression also needs further study. Dysregulation of ET-1 in Hispanic/Latino populations largely have been unexplored. Genetics linking ET-1 dysregulation and racial disparities in AD also needs further investigation. In this review, I examine how AD effects underserved minority populations and how dysregulation of the ET-1 system specifically predisposes ethnic minorities to AD. In addition, I examine the molecular interactions of the ET-1 system and amyloid beta, the role the ET-1 system in neurodegeneration, potential therapeutics for ET-1 dysregulation, and the impact on AD progression.
Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma
Authors: Jin Sun Woo , Lee Gi Ho , Jang Min Jung , Hong Gyeong Eun , Kim Jae Young , Park Gi Deok , Jin Hui , Kim Hyun Su , Choi Chul Yung , Choi Jae Ho , Lee Su Gwon , Jeong Hye Gwang , Hwang Yong Pil
Published: October 2020

Several air pollution components such as sulfur dioxide, ozone, nitrogen dioxide, and diesel exhaust particulate matter (DEPM) have been linked to the development of asthma. In this study, we investigated the therapeutic potential of three lactic acid bacteria species, Lactobacillus plantarum GREEN CROSS Wellbeing (GCWB)1001, Pediococcus acidilactici GCWB1085, and Lactobacillus rhamnosus GCWB1156, in preventing DEPM-exacerbated asthma in mice. BALB/c mice were first sensitized with ovalbumin (OVA) and were either challenged with OVA or DEPM (DEPM-exacerbated asthma model) by intranasal instillation. All three strains showed no hemolytic activity, suggesting a good safety profile. Oral administration of lactic acid bacteria reduced OVA + DEPM-induced inflammatory infiltration, goblet cell hyperplasia, airway remodeling, and the levels of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF). The probiotics also attenuated OVA + DEPM-induced immunoglobulin E (IgE) levels in serum and in BALF, and significantly reduced caspase-3 activity, total collagen level, and matrix metalloproteinase (MMP)-9 activity. In conclusion, lactic acid bacteria such as L. plantarum GCWB1001, P. acidilactici GCWB1085, and L. rhamnosus treatment in mice with asthma showed significant efficacy in preventing lung inflammation exacerbated by DEPM administration.
Joining Alumina to Titanium Alloys Using Ag-Cu Sputter-Coated Ti Brazing Filler
Authors: Emadinia Omid , Guedes Aníbal , Tavares Carlos José , Simões Sónia
Published: October 2020

The joining of alumina (Al2O3) to γ-TiAl and Ti6Al4V alloys, using Ag-Cu sputter-coated Ti brazing filler foil, was investigated. Brazing experiments were performed at 980 °C for 30 min in vacuum. The microstructure and chemical composition of the brazed interfaces were analyzed by scanning electron microscopy and by energy dispersive X-ray spectroscopy, respectively. A microstructural characterization of joints revealed that sound multilayered interfaces were produced using this novel brazing filler. Both interfaces are composed mainly of α-Ti, along with Ti2(Ag,Cu) and TiAg intermetallics. In the case of the brazing of γ-TiAl alloys, α2-Ti3Al and γ-TiAl intermetallics are also detected at the interface. Bonding to Al2O3 is promoted by the formation of a quite hard Ti-rich layer, which may reach a hardness up to 1872 HV 0.01 and is possibly composed of a mixture of α-Ti and Ti oxides. Hardness distribution maps indicate that no segregation of either soft or brittle phases occurs at the central regions of the interfaces or near the base Ti alloys. In addition, a smooth hardness transition was established between the interface of Al2O3 to either γ-TiAl or Ti6Al4V alloys.
The Influence of the Continuous Casting Conditions on the Properties of High-Strength Two-Phase CuMg Alloys
Authors: Strzępek Paweł , Mamala Andrzej , Zasadzińska Małgorzata , Noga Piotr , Sadzikowski Michał
Published: October 2020

Constant tendency toward the improvement of the material properties nowadays creates opportunities for the scientists all over the world to design and manufacture new alloys almost every day. Considering the fact that companies all over the world desire alloys with the highest values of mechanical properties often coexisting with a reasonable electrical conductivity made it necessary to develop new materials based on Cu, such as CuMg alloys. However, before such new material may be mass produced it must undergo a series of tests in order to determine the production technology, its parameters and influence on the chemical composition, microstructural properties, and both mechanical and physical properties of CuMg alloys. The research tests have shown that with the increase of the casting feed the Brinell’s hardness of each material slightly increases (by 5 HB2.5/62.5). There is little to none impact of the casting feed on the electrical conductivity, values of which are between 20.6 and 21.4 MS/m (around 40% IACS-International Annealed Copper Standard) depending on the Mg content. The conducted scanning electron microstopy (SEM) analysis has shown that the magnesium precipitations are evenly distributed among the volume of the alloy, however, a significant difference in the density and shape of the Cu + Cu2Mg aggregates was noticed regarding various casting feed. Static compression test proved that these alloys may be subjected to strain hardening as the hardness of the material after compression increases by approximately 40 HB2.5/62.5.
Comparison of 3D-Printed Dental Implants with Threaded Implants for Osseointegration: An Experimental Pilot Study
Authors: Li Ling , Lee Jungwon , Amara Heithem Ben , Lee Jun-Beom , Lee Ki-Sun , Shin Sang-Wan , Lee Yong-Moo , Kim Byoungkook , Kim Pangyu , Koo Ki-Tae
Published: October 2020

This study aimed to compare bone healing and implant stability for three types of dental implants: a threaded implant, a three-dimensional (3D)-printed implant without spikes, and a 3D-printed implant with spikes. In four beagle dogs, left and right mandibular premolars (2nd, 3rd, and 4th) and 1st molars were removed. Twelve weeks later, three types of titanium implants (threaded implant, 3D-printed implant without spikes, and 3D-printed implant with spikes) were randomly inserted into the edentulous ridges of each dog. Implant stability measurements and radiographic recordings were taken every two weeks following implant placement. Twelve weeks after implant surgery, the dogs were sacrificed and bone-to-implant contact (BIC) and bone area fraction occupied (BAFO) were compared between groups. At implant surgery, the primary stability was lower for the 3D-printed implant with spikes (74.05 ± 5.61) than for the threaded implant (83.71 ± 2.90) (p = 0.005). Afterwards, no significant difference in implants’ stability was observed between groups up to post-surgery week 12. Histomorphometrical analysis did not reveal a significant difference between the three implants for BIC (p = 0.101) or BAFO (p = 0.288). Within the limits of this study, 3D-printed implants without spikes and threaded implants showed comparable implant stability measurements, BIC, and BAFO.
Titanium Dioxide Thin Films Obtained by Atomic Layer Deposition Promotes Osteoblasts’ Viability and Differentiation Potential While Inhibiting Osteoclast Activity—Potential Application for Osteoporotic Bone Regeneration
Authors: Smieszek Agnieszka , Seweryn Aleksandra , Marcinkowska Klaudia , Sikora Mateusz , Lawniczak-Jablonska Krystyna , Witkowski Bartlomiej. S. , Kuzmiuk Piotr , Godlewski Marek , Marycz Krzysztof
Published: October 2020

Atomic layer deposition (ALD) technology has started to attract attention as an efficient method for obtaining bioactive, ultrathin oxide coatings. In this study, using ALD, we have created titanium dioxide (TiO2) layers. The coatings were characterised in terms of physicochemical and biological properties. The chemical composition of coatings, as well as thickness, roughness, wettability, was determined using XPS, XRD, XRR. Cytocompatibillity of ALD TiO2 coatings was accessed applying model of mouse pre-osteoblast cell line MC3T3-E1. The accumulation of transcripts essential for bone metabolism (both mRNA and miRNA) was determined using RT-qPCR. Obtained ALD TiO2 coatings were characterised as amorphous and homogeneous. Cytocompatibility of the layers was expressed by proper morphology and growth pattern of the osteoblasts, as well as their increased viability, proliferative and metabolic activity. Simultaneously, we observed decreased activity of osteoclasts. Obtained coatings promoted expression of Opn, Coll-1, miR-17 and miR-21 in MC3T3-E1 cells. The results are promising in terms of the potential application of TiO2 coatings obtained by ALD in the field of orthopaedics, especially in terms of metabolic- and age-related bone diseases, including osteoporosis.
Bending Fatigue Behavior of 316L Stainless Steel up to Very High Cycle Fatigue Regime
Authors: Hu Yongtao , Chen Yao , He Chao , Liu Yongjie , Wang Qingyuan , Wang Chong
Published: October 2020

Effect of microstructure on the crack initiation and early propagation mechanism in the very high cycle fatigue (VHCF) regime was studied in 316L stainless steel (316L SS) by atomic force microscope (AFM) and electron back scattered diffraction (EBSD). The results show that small fatigue cracks initiate from the slip band near the grain boundaries (GBs) or the twin boundaries (TBs). Early crack propagation along or cross the slip band is strongly influenced by the local microstructure such as grain size, orientation, and boundary. Besides, the gathered slip bands (SBs) are presented side by side with the damage grains of the run-out specimen. Finally, it is found that dislocations can either pass through the TBs, or be arrested at the TBs.
WEDM Used for Machining High Entropy Alloys
Authors: Mouralova Katerina , Benes Libor , Zahradnicek Radim , Bednar Josef , Zadera Antonin , Fries Jiří , Kana Vaclav
Published: October 2020

Unconventional wire electrical discharge machining technology (WEDM) is a key machining process, especially for machining newly emerging materials, as there are almost no restrictions (only at least minimal electrical conductivity) in terms of demands on the mechanical properties of the workpiece or the need to develop new tool geometry. This study is the first to present an analysis of the machinability of newly developed high entropy alloys (HEAs), namely FeCoCrMnNi and FeCoCrMnNiC0.2, using WEDM. The aim of this study was to find the optimal setting of machine parameters for the efficient production of parts with the required surface quality without defects. For this reason, an extensive design of experiments consisting of 66 rounds was performed, which took into account the influence of five input factors in the form of pulse off time, gap voltage, discharge current, pulse on time, and wire speed on cutting speed and the quality of the machined surface and its subsurface layer. The analysis of topography, morphology, subsurface layers, chemical composition analysis (EDX), and lamella analysis using a transmission electron microscope (TEM) were performed. An optimal setting of the machine parameters was found, which enables machining of FeCoCrMnNi and FeCoCrMnNiC0.2 with the required surface quality without defects.
The Influence of Multiple Pass Submerged Friction Stir Processing on the Microstructure and Mechanical Properties of the FSWed AA6082-AA8011 Joints
Authors: Mabuwa Sipokazi , Msomi Velaphi
Published: October 2020

The AA6082–AA8011 friction stir-welded joints were subjected to submerged multiple pass friction stir processing to evaluate the microstructure and mechanical properties of the joints. A maximum of four submerged friction stir processed passes were used in this study. All the specimens were extracted from three different joint positions (start, middle and end). The tests conducted included microstructural analysis, tensile tests, hardness and fracture surface morphology of the post-tensile specimens, were performed using a scanning electron microscope (SEM). There was no particular trend in the microstructure and mechanical properties when looking at the specimen positioning in all the passes. The minimum mean grain sizes were refined from 3.54 to 1.49 µm and the standard deviation from 5.43 to 1.87 µm. The ultimate tensile strength was improved from 84.96 to 94.77 MPa. The four-pass SFSPed specimens were found to have more ductile properties compared to the one-pass SFSPed one. The hardness of the stir zones in all the passes was found to be higher compared to the AA8011 base material but lower than the AA6082 one. The maximum stir zone hardness of 75 HV was observed on the one-pass SFSP joints.