bluuebunny/crossref_metadata_2025 huggingface dataset:
Nano-chitosan Spray as a Preservative and Food Security of Fishery Products in The Middle of the Covid-19 Pandemic
Authors: Saputra Dion , Ula Ferra Robiatul , Fadhila Ajeng Budiarahma Nur , Sijabat Yosi Yananda , Romadoni Abista Ahmad , Windarto Seto
Published: March 2022

Highlight ResearchThe author mentioned 2 highlights from their results research  Nano-chitosan has the best susceptibility to bacterial strains of E. coli, S. enteritidis, L. monocytogenes, B. cereus and S. aureus.Nano-chitosan showed higher degree of inhibition than that done by chitosan. AbstractThe COVID-19 pandemic has impacted the fisheries sector, a decline in exports and fishermen's income caused by the disconnection of the marketing chain due to lockdown implementation in several export destination countries. Fish is a source of protein and as perishable goods, it experiences quality damage due to spoilage, commonly caused by hampered distribution of catches. Natural preservatives are needed to preserve catch so it won’t get spoiled and can be accepted by consumers in suitable conditions for consumption. This study aims to evaluate the particle size of nano-chitosan and determine the effectiveness of nano-chitosan spray with different concentrations as a natural preservative in caught fish. This research method begins with making chitosan through 3 stages: demineralization, deproteination, and deacetylation. Chitosan was made into nano-chitosan with various concentrations of 3:1, 4:1, and 5:1 using ionic gelation. Nano-chitosan underwent several tests, including PSA, antibacterial activity by disc diffusion, organoleptic, and the effectiveness of preservatives. Antibacterial activity of nano-chitosan was able to inhibit Bacillus subtilis at three different concentrations, the potential to inhibit Escherichia coli was optimal at 5:1 treatment. The effectiveness of nano-chitosan preservative bacteria inhibition at three different concentrations proved to be sufficient to be used to extend shelf life and ensure the safety and quality of fishery products. The best concentration of nano-chitosan was 3:1 treatment. Nano-chitosan spray from crab shell waste has good antibacterial activity and preservative effectiveness. It could serve as an antibacterial agent and natural preservative for fishery products during the COVID-19 pandemic.
Marine Biodiversity of Coral Reef Fishes in Pieh Marine Recreational Park After Bleaching and Acanthaster Outbreaks
Authors: Putra Risandi Dwirama , Abrar Muhammad , Siringoringo Rikoh Manogar , Purnamsari Ni Wayan , Agustina Pradipta , Islam MD Jayedul
Published: March 2022

Highlight ResearchAfter bleaching disturbance and Acanthaster planci outbreaks, herbivore fishes species were dominated in MRP PiehThis explains the marine recreational activities in utilization zone MRP Pieh have no impact on reducing fish biomassAfter disturbance in MRP Pieh shows the coral reef fishes still endurance on bleaching event, and Acanthaster planci outbreak after disturbance in MRP Pieh shows the coral reef fishes still endurance on bleaching event and Acanthaster planci outbreak. However, the composition of corallivorous has decreased but has been an increase in herbivorous and carnivorous fish populationsAbstractPieh MRP encountered non-anthropogenic phenomena, precisely massive coral bleaching in 2016, 2017, and massive predators from Acanthaster planci outbreaks in 2018. This study aimed to understand the pattern of coral reef diversity in the core zone and utilization in the MRP area and compare it to non-MRPS locations that accept the same non-anthropogenic pressure conditions. Coral fish sampling using a UVC is categorized into three zones: the core zone, the utilization zone, and outside the MRP area. 8 Families of coral reef fishes were counted based on categories of level function in ecologies and economy. Taxonomic distinctiveness estimates were calculated mathematically for each sample, including species richness and taxonomic diversity were compared among zonation area. Pearson’s Coefficient Correlation Matrix was used to measure the correlation relationship between zonation areas. There are 91 species of fish and 3002 individuals found. The richest family in the MRP Core Zone and MRP Utility Zone was Acanthuridae with 20 species and non-MRP has a lower species richness and abundance of fish communities. The dominant species in Pieh MRP was Ctenochaetus striatus with average abundant per site (21.3 ± 7.62, n = 3). Acanthuridae represents 55.98% of the total biomass in MRP-Core Zone, 63.13% in MRP-Utility Zone, and 41.55% in Non-MRP Area. This study showed the number of species and populations from corallivores fishes have decreased but has been an increase in herbivorous and carnivore diversity. The diversity indices (H’) and ENS also shows no differ significantly between zonation.
Morphometric and Meristic Characterization of Hairtails Trichiurus lepturus Linnaeus, 1758 (Scombriformes: Trichiuridae) from the Northern Coast of Java, Indonesia
Authors: Aryantojati Adhimas Febri , Murwantoko Murwatoko , Setyobudi Eko
Published: March 2022

Highlight ResearchThe most significant morphological variation was the snout length and caudal peduncleT. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same populationT. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species AbstractHairtails (Trichiurus spp.) are economically important fisheries resources and have become an export commodity. In Indonesia, there are several hairtail species; however, each species distribution is not yet certainly known. This study aimed to identify and determine the morphometric and meristic character of the hairtail from the northern coast of Java, Indonesia. Fish specimens were obtained from fishermen catches in the north coast of Pati and Demak (Central Java) and the northern coast Cirebon (West Java), which captures the fish in less than one-week fishing operation. Fish samples were grouped based on morphological similarities identified in previously published works. After that, 22 morphometric and four meristic characters were measured and counted. Principal Component Analysis and Discriminant Analysis were used to analyze morphometric data, while the meristic data were compared to the available published works. The hairtail caught at the northern coast of Java was long, flat, silvery in color, and had a sagittal crest, with the fin formula being D.III.125-134, P.I.10, and A.I.98-104. According to the morphological and meristic characters, the hairtail caught at Java’s northern coast was identified as Trichiurus lepturus. The most significant morphological variation was the snout length and caudal peduncle. The PCA and scattergram analyses showed that T. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same population.  T. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species.
Epidemiological characteristics of suicide mortality in Turkey and trend over the years, 2007-2019
Authors: TEKER Ayşe Gülsen
Published: February 2022

Objective: Suicide is an important public health problem today. Accurate, complete, timely, and rapid registration and evaluation of data about suicide will form the basis for preventive interventions to be developed. This study aims to determine the epidemiological features of suicide mortality in Turkey between 2007-2019 and examine the trend of suicide mortality rates. Methods: In this study, the Turkish Statistical Institute (TURKSTAT) data was used, all deaths related to suicide in Turkey between 2007-2019 were analyzed. The change in age-adjusted suicide-related mortality rates over time was evaluated by Jointpoint regression analysis. Results: Between 2007 and 2019, the age standardised suicide-related mortality rates in males increased by an average of 1.3 percent annually, and this change was statistically significant (p
The Predicament and Countermeasures of English Writing in Senior High School Teaching
Authors: Li Yanrong
Published: February 2022

As an important part of high school English classroom teaching, writing teaching is one of the important ways to improve students' English learning ability. As for the current high school English writing teaching, there are still many problems in classroom learning. For example, the students' writing efficiency is not high, the number of words in the classroom writing practice is not enough, off-topic, and writing interest is not high are the practical problems that high school teachers need to solve urgently.
Analyzing the Reversal of Public Opinion in the Internet Era From "Bao Yuming's Alleged Sexual Assault Case
Authors: Yao Yuan , Sun Wenzhi
Published: February 2022

With the rapid development of the Internet in China in recent years, the public tends to get information about news events from the Internet and publish their opinions on online social platforms. Guiding people's public opinion in the online world has become an essential social proposition. We choose the "Bao Yuming's alleged sexual assault case," which caused an intense social discussion on the Chinese Internet in 2020, as a case study for analysis and analysis of the various public opinion phenomena that emerged in society at that time discuss their causes and background. Finally, we propose ways to guide the case from three aspects.
Research on the Legitimacy of the Resale Right
Authors: Yuan Zhengrong , Zhang Chong
Published: February 2022

The resale right system, first created in France in 1920, has a history of one hundred years and has been confirmed by dozens of national legislations. In general, the introduction of the resale right has become a trend in the legislation of various countries. But the universal establishment of the resale right does not necessarily mean that it has legitimacy. On the issue of whether the introduction of the resale right is justified, the views of supporters and opponents are tit-for-tat, and several major points of controversy have formed. By sorting out the disputes involved in the resale right system, clarifying the core problems of introducing the resale right, and demonstrating its legitimacy, it is reasonable and feasible to introduce the resale right.
A Study of American TV Series Subtitle Translation From the Perspective of Translation Variation Theory
Authors: Yuan Chenjun
Published: February 2022

In the Internet age, the spread of overseas film and television dramas is increasing day by day, and the high-quality requirements of domestic audiences for subtitle translation of film and television dramas need to be paid great attention by the translation community. Translation Variation Theory emphasizes that translators should translate according to the specific needs of readers, and the audience needs of subtitle translation are more diversified, so the theory is suitable for subtitle translation which requires extremely high flexibility. The author will explore the subtitle translation of American TV series from the perspective of Translation Variation Theory, and take Friends as an example to analyze the reasons why subtitle translation conforms to the Translation Variation Theory, so as to provide reference for future subtitle translation studies.
The Present Situation and Re-protection of Intangible Cultural Heritage NiNigou
Authors: Ruan Chunxin
Published: February 2022

As an intangible cultural heritage, NiNigou has its body made of yellow soil and its clothes made of colorful dot painting on black background. The patterns painted on its body are not only the embodiment of artistic quality, but also contain the totem culture of thousands of years of human civilization and wisdom. Therefore, the author led the social practice team to Huaiyang, the birthplace of NiNigou. By visiting Mr. Ren Guohe, the inheritor of NiNigou culture, I had an in-depth understanding of the production process of NiNigou, the significance of cultural symbols and the contemporary value, so as to explore how to better protect and inherit the intangible cultural heritage of NiNigou.
Binary Data Matrix Theory
Authors: Amiri Manouchehr
Published: March 2022

In this paper after introducing a model of binary data matrix (BDM) for physical parameters of an evolving system (of particles), we develop a Hilbert space as an ambient space to derive induced metric tensor on embedded parametric manifold identified by associated joint probabilities of particles observables (parameters). Parameter manifold assumed as space-like hypersurface evolving along time axis, an approach that resembles 3+1 formalism of ADM and numerical relativity. We show the relation of endowed metric with related density matrix. Identification of system density matrix by this metric tensor, leads to the equivalence of quantum Liouville equation and metric compatibility condition while covariant derivative of metric tensor has been calculated respect to Wick rotated time or spatial coordinates. After deriving a formula for expected energy per particles, we prove the equality of this expected energy with local scalar curvature of related manifold. We show the compatibility of BDM model with Hamilton-Jacobi formalism and canonical forms. On the basis of the model, I derive the Ricci flow like dynamics as the governing dynamics and subsequently derive the action of BDM model and Einstein field equations. Given examples clarify the compatibility of the results with well-known principles such as equipartition energy principle and Landauer’s principle. This model provides a background for geometrization of quantum mechanics compatible with curved manifolds and information geometry. Finally, we conclude a “bit density principle” which predicts the Planck equation, De Broglie wave particle relation, , Beckenstein bound and Bremermann limit.
Biological Effects of Non-ionizing Electromagnetic Fields at 27 GHz on Sperm Quality of Mytilus galloprovincialis
Authors: Pecoraro Roberta , Pavone Santi Concetto , Scalisi Elena Maria , Sica Carmen , Ignoto Sara , Contino Martina , Salvaggio Antonio , Sorbello Gino , Di Donato Loreto , Brundo Maria Violetta
Published: March 2022

Recently, a rising use of wireless internet technologies has been demonstrated. The devices 18 which use these technologies emit in new spectral regions an electromagnetic radiation (EMFs) 19 which could interact with the male reproductive system. The aim of this study was to investigate in 20 vitro influence of electromagnetic fields at 27 GHz on sperm quality in Mytilus galloprovincialis. 21 Sperm samples, were collected from sexually mature males of M. galloprovincialis and placed in sea- 22 water. Once evaluated the number and quality of spermatozoa, sperm cells were exposed to elec- 23 tromagnetic fields radiated by a pyramidal horn antenna. The effect of exposure was evaluated after 24 10, 20, 30, 40 and 60 minutes with light microscope and using Eosin test. Ten replications were per- 25 formed for each time series, and statistical analysis was carried out byt-test. A significative decrease 26 in sperm motility was observed after 10 minutes of exposure and after 30 minutes most of sperms 27 were immobile and not vital. This study provides useful data on potential ecological impact of the 28 5G high-band on animal fertility, whose effect is currently under investigation.
Peak and Cumulative Response of Reinforced Concrete Frames with Steel Damper Columns under Seismic Sequences
Authors: Fujii Kenji
Published: March 2022

The steel damper column is an energy-dissipating member that is suitable for reinforced concrete (RC) buildings, and those used for multistory housing in particular. However, the effectiveness of steel damper columns may be affected by the behavior of surrounding members, and this effect can be severe in the case of seismic sequences. This article investigates the nonlinear response of building models having an RC moment-resisting frame (MRF) with and without steel damper columns under seismic sequences. The applicability of the concept of the momentary energy input to the prediction of the peak response of RC MRFs with damper columns under seismic sequences is also investigated. The main findings of the study are summarized as follows. (1) The peak response of RC MRFs with damper columns subjected to sequential accelerations is similar to the peak response obtained considering only the mainshock, whereas the cumulative strain energy of RC MRFs accumulates more for sequential accelerations. (2) The steel damper column is effective in reducing the peak and cumulative responses of RC MRFs in the case of sequential seismic input. (3) The relation of the hysteretic dissipated energy during a half cycle of the structural response and the peak displacement of the first modal response can be properly evaluated using the simple model proposed in this study.
Preclinical Studies of Granulysin-Based Anti-Tn Immunotoxins as A New Antitumoral Treatment
Authors: Guerrero-Ochoa Patricia , Ibáñez-Pérez Raquel , Berbegal-Pinilla Germán , Aguilar Diederich , Marzo Isabel , Corzana Francisco , Conde Blanca , Raso Javier , Hurtado-Guerrero Ramón , Anel Alberto
Published: March 2022

Two granulysin (GRNLY) based immunotoxins were generated, one containing the scFv of the SM3 mAb (SM3GRNLY) and the other the scFv of the AR20.5 mAb (AR20.5GRNLY). These mAb recognize different amino acid sequences of aberrantly O-glycosilated MUC1, also known as the Tn antigen, expressed in a variety of tumor cell types. We first demonstrated the affinity of these immunotoxins for their antigen using surface plasmon resonance for the purified antigen and flow cytometry for the antigen expressed on the surface of living tumor cells. The induction of cell death of tumor cell lines of different origin positive for Tn antigen expression was stronger in the cases of the immunotoxins than that induced by GRNLY alone. The mechanism of cell death induced by the immunotoxins was studied, showing that the apoptotic component demonstrated previously for GRNLY was also present, but that cell death induced by the immunotoxins included also necroptotic and necrotic components. Finally, we demonstrated the in vivo tumor targeting by the immunotoxins after systemic injection using a xenograft model of the human pancreatic adenocarcinoma CAPAN-2 in athymic mice. While GRNLY alone did not have a therapeutic effect, SM3GRNLY and AR20.5GRNLY reduced tumor volume by 42 and 60%, respectively, compared with untreated tumor-bearing mice, although the results were not statistically significant in the case of AR20.5GRNLY. Histological studies of tumors obtained from treated mice demonstrated reduced cellularity, nuclear morphology compatible with apoptosis induction and active caspase-3 detection by immunohistochemistry. Overall, our results exemplify that these immunotoxins are potential drugs to treat Tn-expressing cancers.
An Overview on Green Synthesis of Nanomaterials and Their Advanced Applications in Sustainable Agriculture
Authors: Aslam Aamir Ali , Aslam Awais Ali , Aslam Muhammad Suhail , Quazi Sameer
Published: March 2022

The excess use of unsafe pesticides and mineral fertilizers in agriculture has led to serious health problems and environmental pollution. Nanotechnology has been solving these problems by providing nanoparticles (NPs) with excellent performance. By green synthesis of nanoparticles from plants, animals, and microbes, the use of hazardous and toxic chemicals has become limited. Nanoparticles have excellent performance in many fields such as electronics, cosmetics, automobiles, catalysis, biosensors, bioengineering, etc. NPs also showed excellent performance in agriculture by improving crop production and food quality. Various nano-based agroparticles that have conducted many smart and efficient agricultural systems involving nanopesticides, nanofertilizers, nanoherbicides etc. Apart from enhancing the food production, these materials operate some other functions like as identifying disease in plants, control release of nutrients, delivery of nutrients at target sites, etc. various nanofertilizers such as Fe, Mn, N, K, Mo, P, CNTs and P showed excellent targeted delivery performance. Nanopesticides and many nanoformulations have showed excellent pest protection performance. Here we reviewed the sources of nanomaterials and their excellent performance in agriculture.
Visualization and Comprehension of Electronic and Topographic Contrasts on Cooperatively Switched Diarylethene-Bridged Ditopic Ligand
Authors: Hnid Imen , Guan Lihao , Chatir Elarbi , Cobo Saioa , Lafolet Frederic , Maurel Francois , Lacroix Jean-Christophe , Sun Xiaonan
Published: March 2022

Diarylethene is a prototypical molecular switch that can be reversibly photoisomerized between its open and closed forms. Ligands bpy-DAE-bpy, consisting of a phenyl-diarylethene-phenyl (DAE) central core and bipyridine (bpy) terminal substituents, are able to self-organize. They are investigated by scanning tunneling microscopy at the solid-liquid interface. Upon light irradiation, cooperative photochromic switching of the ligands is recognized down to the sub-molecular level. The closed isomers show different electron density of states (DOS) contrasts, attributed to the HOMO or LUMO molecular orbitals observed. More importantly, the LUMO images show remarkable differences between the open and closed isomers, attributed to combined topographic and electronic contrasts mainly on the DAE moieties. The electronic contrasts from multiple HOMO or LUMO distributions, combined with topographic distortion of the open or closed DAE, are interpreted by density functional theory (DFT) calculations.
Enteroinvasive Escherichia Coli is Inefficient to Escape from Autophagy during Caco-2 Cellular Infection
Authors: Bando Silvia Yumi , Moreno Ana Carolina Ramos , Santos Hadassa Cristhina de Azevedo Soares dos , Bertonha Fernanda Bernardi , Ferreira Lucas Gonçalves , Martinez Marina Baquerizo , Moreira-Filho Carlos Alberto
Published: March 2022

Escherichia coli and Shigella are common diarrhea-causing pathogens in children and adults. Enteroinvasive Escherichia coli (EIEC) shares a similar pathogenic mechanism with Shigella. However, EIEC are less virulent than Shigella. The aim of this work was to get a better understanding of the virulence differences between EIEC and S. flexneri. We investigated i) the bacterial gene co-expression networks (GCNs) and ii) the the transcriptional modules (WGCNA) of Caco-2 cells infected with EIEC or with S. flexneri during a three-hour period of bacterial infection. The GCN analysis showed that EIEC and S. flexneri networks presented different topologies. Additionally, the EIEC network revealed that pINV genes are not connected with chromosomal genes. WGCNA and eigengene analysis showed enterocyte gene expression variation along the three-hour bacterial post-infection period. Additionally, at one-hour post-infection EIEC induced a higher number of gene expression changes in Caco-2 cells than S. flexneri. Several of these genes are involved in autophagy. This study showed that the lower virulence of EIEC is associated with a lack of functional cooperation between pINV and chromosomal genes, differently from what was observed in S. flexneri. Consequently, EIEC becomes less efficient in subverting host-cell bacterial recognition as well as defense mechanisms such as autophagy.
A Generalized Measure of Cumulative Residual Entropy
Authors: Kattumannil Sudheesh Kumar , Sreedevi E. P. , Balakrishnan N.
Published: March 2022

In this work, we introduce a generalized measure of cumulative residual entropy and study its properties. We show that several existing measures of entropy such as cumulative residual entropy, weighted cumulative residual entropy and cumulative residual Tsallis entropy, are all special cases of the generalized cumulative residual entropy. We also propose a measure of generalized cumulative entropy, which includes cumulative entropy, weighted cumulative entropy and cumulative Tsallis entropy as special cases. We discuss generating function approach using which we derive different entropy measures. Finally, using the newly introduced entropy measures, we establish some relationships between entropy and extropy measures.
Evaluation of Face Shields, Goggles and Safety Glasses as a Virus Transmission Control Measure to Protect the Wearer Against Cough Droplets
Authors: Hall Samantha , Johnson Paul , Bailey Claire , Gould Zoe , White Robert , Crook Brian
Published: March 2022

Face shields (also referred to as visors), goggles and safety glasses have been worn during the COVID-19 pandemic as one measure to control transmission of the virus. However, their effectiveness in controlling facial exposure to cough droplets is not well established and standard tests for evaluating eye protection for this application are limited. A method was developed to evaluate face shields, goggles and safety glasses as a control measure to protect the wearer against cough droplets. The method uses a semi-quantitative assessment of facial droplet deposition. A cough simulator was developed to generate droplets comparable to those from a human cough. The droplets consisted of a UV fluorescent marker (fluorescein) in water. Fourteen face shields, four pairs of goggles and one pair of safety glasses were evaluated by mounting them on two different sizes of breathing manikin head and challenging them with the simulated cough. The manikin head was positioned in seven orientations relative to the cough simulator to represent various potential occupational exposure scenarios, for example, a nurse standing over a patient. Droplet deposition in the eyes, nose and mouth regions were visualised following three ‘coughs’. Face shields, goggles and safety glasses reduced, but did not eliminate exposure to the wearer from droplets such as those produced by a human cough. The level of protection differed based on the design of the personal protective equipment and the relative orientation of the wearer to the cough. For example, face shields and goggles offered the greatest protection when a cough challenge was face on or from above and the least protection when a cough challenge was from below. Face shields were also evaluated as source control to protect others from the wearer. Results suggested that if a coughing person wears a face shield, it can provide some protection from cough droplets to those standing directly in front of the wearer.
Horticulture Research In Central Asia: A review of Papers from Scopus Database Published for The Period of 2000-2020
Authors: Rajametov Sherzod , Abdullaev Sadulla
Published: March 2022

Horticulture crops (fruit trees) had been grown and cultivated from ancient times in Central Asia. Few researchers have addressed the problem of this profitable sector in the former Great Silk Road, which was at the crossroads of trading avenues. Horticulture has received much attention in the last twenty years. To investigate the current state of research activity of horticulture in Central Asia, we downloaded 4205 English papers from the Scopus database between 2000-2020. We identified a total of 50 papers, and the last four years have witnessed significant growth in publication number, an average of 5 articles per year. Acta Horticulturea was one of the most productive journal. Institute of Plant Biology and Biotechnology (Almaty) and the Academy of Sciences of the Republic of Uzbekistan have worked productively to study high issues. United States Department of Agriculture and Swiss National Science Foundation with Karl Popper Foundation have supported scientific activity in the region. Top 15 highly cited articles were published within the framework of funded projects with international researchers. Researchers of Central Asia focused on walnut, grape and apple, studied on molecular level and cryopreservation of wild relatives for future use. Cherry, apricot, almond and pomegranate crops were less studied by researchers.
Factors Associated with COVID-19 Vaccine Booster Hesitancy: a Retrospective Cohort Study, Fukushima Vaccination Community Survey
Authors: Yoshida Makoto , Kobashi Yurie , Kawamura Takeshi , Shimazu Yuzo , Nishikawa Yoshitaka , Omata Fumiya , Zhao Tianchen , Yamamoto Chika , Kaneko Yudai , Nakayama Aya , Takita Morihito , Ito Naomi , Kawashima Moe , Sugiura Sota , Shibuya Kenji , Iwami Shingo , Kim Kwangsu , Iwanami Shoya , Kodama Tatsuhiko , Tsubokura Masaharu
Published: March 2022

This was a retrospective cohort study, which aimed to investigate the factors associated with hesitancy to receive the third dose of coronavirus disease 2019 (COVID-19) vaccine. A paper-based questionnaire survey was administered to all participants. Accordingly, the study included participants who provided answer in the questionnaire whether they have an intent to receive the third dose of vaccine. Data on sex, age, area of residence, adverse reactions after the second vaccination, whether the third vaccination was desired, and reasons to accept or hesitate booster vaccination were retrieved. Among the 2439 participants with mean (±SD) age of 52.6±18.9 years, and median IgG-S antibody titer of 324.9 (AU/mL), 97.9% of participants indicated their intent to accept a third vaccination dose. The logistic regression revealed that younger age (OR=0.98; 95% CI: 0.96-1.00) and higher antibody level (OR=2.52; 95% CI: 1.27-4.99) are positively associated with the third vaccine hesitancy. The efficacy of the COVID-19 vaccine and concerns about adverse reactions had significant impact on the third vaccination behavior. A rapid increase in the booster dose rate is needed to control the pandemic, and specific approaches should be taken in these groups that are likely to hesitate the third vaccine, subsequently increasing booster contact rate.
Machine Learning-Powered Models for Near-Infrared Spectrometers: Prediction of Protein in Multiple Grain Cereals
Authors: Chadalavada Keerthi , Anbazhagan Krithika , Ndour Adama , Choudhary Sunita , Palmer William M. , Flynn Jamie R. , Mallayee Srikanth , Sharada P. , Prasad K.V.S.V. , Padmakumar V. , Jones Chris , Kholová Jana
Published: March 2022

Achieving global goals on sustainable nutrition, health, and wellbeing will depend on delivering enhanced diets to humankind. This will require, among others, instantaneous access to information on food quality at key points within agri-food systems. Although stationary methods are usually used to quantify grain quality (wet-lab chemistry, benchtop NIR spectrometer); these do not suit many required user-cases, such as stakeholders in decentralized agri-food-chains that are typical for emerging economies. Therefore, we explored new technologies and models that might aid these particular user-cases. For this purpose, we generated the NIR spectra of 328 grain samples from multiple cereals (finger millet, foxtail millet, maize, pearl millet, sorghum) with a standard benchtop NIR Spectrometer (DS2500, FOSS) and a novel mobile NIR-based sensor (HL-EVT5, Hone). We explored a range of classical deterministic and novel machine learning (ML)-driven models to build calibrations out of the NIR spectra. We were able to build relevant calibrations out of both types of spectra. At the same time, ML-based methods enhanced the prediction capacity of calibration models compared to classical deterministic methods. We also documented that the prediction of grain protein content based on NIR spectra generated by a mobile sensor (HL-EVT5, Hone) was highly relevant for quantitative protein predictions (R2 = 0.91, RMSE = 0.97, RPD = 3.48). Thus, the findings of this study lay the foundations on which to expand the utilization of NIR spectroscopy applications for agricultural research and development.
Probing the Potential of Community-Based Transportation Facilities: A Case of Community Managed School Van Service in Dhaka City
Authors: Das Anutosh , Akter Sharmin , Roy Sumita , Shahriyar Parvez Mohammad
Published: March 2022

Social safety, security, and comfort of school-going children during the travel time to school becomes a subject of anxiety to the parents and is a crucial issue in recent times. In this regard, community-based transport can be a significant way to address social security issues in travel at a reasonable cost and reduce the burden on private mode. In Dhaka city, school van service already exists but due to some sort of problems the service has not been proved an efficient and formal mode of transport for solving mobility problems. This study seeks to identify the existing problems and prospects of the school van service and provide a unique, healthy, safe, and reliable transport mode for children. Applying different tools of the Participatory Rural Appraisal (PRA) method, the problems and solutions have been drawn from the community. The recommendations of this study will help the school van services (a community-managed para-transit system) to be more functional in playing a vital role in solving the problems of short-distance travel. This service has great potentialities to be adopted in other trips such as trips to and from offices which will lessen the road congestion at the peak periods.
Challenges to Use Machine Learning in Agricultural Big Data: A Systematic Literature Review
Authors: Cravero Ania , Pardo Sebastian , Sepúlveda Samuel , Muñoz Lilia
Published: March 2022

Agricultural Big Data is a set of technologies that allows responding to the challenges of the new data era. In conjunction with machine learning, farmers can use data to address different problems such as farmers' decision-making, crops, weeds, animal research, land, food availability and security, weather, and climate change. The purpose of this paper is to synthesize the evidence regarding the challenges involved in implementing machine learning in Agricultural Big Data. We conducted a Systematic Literature Review applying the PRISMA protocol. This review includes 30 papers, published from 2015 to 2020. We develop a framework that summarizes the main challenges encountered, the use of machine learning techniques, as well as the main technologies used. A major challenge is the design of Agricultural Big Data architectures, due to the need to modify the set of technologies adapting the machine learning techniques, as the volume of data increases.
Identification of Novel Direct Small-Molecule Inhibitors against HIF-2A using Structure-based Virtual Screening and Molecular Dynamics Simulation
Authors: Yazdani Behnaz , A.C. Wing Peter , J. Enguita Francisco , Sirous Hajar
Published: March 2022

The main regulatory factors during the adaptation of cancer cells to hypoxic stress are the hypoxia-inducible factors (HIFs), which are being increasingly recognized as an interesting and challenging target for the design of new chemotherapeutic molecules. HIF2A was found to have an large internal hydrophilic cavity within its PAS-B domain, unique to this sub-unit and is suggested to be a possible ligand-binding site. Regulation of HIF2A by cellular molecules is still greatly unknown. In This paper we have employed in-silico techniques, such as molecular docking and dynamic simulation, to design new direct inhibitors against HIF-2A subunit via targeting one of its critical domains and the final top screened molecules have been tested on hypoxic cancer cells for further validation of their inhibitory potential. we targeted the hydrophilic cavity inside the PAS-B domain of the HIF2A to identify novel molecules with a high binding capacity. Virtual Screening methodology was used for molecular docking of NSC library against the target domain inside the HIF2A PAS-B domain with the top 5% compounds with significant MolDock and Re-rank scores were selected for further analysis. The NSC 106416, NSC 217021, NSC 217026, and NSC 215639 compounds were selected based on their docking scores. NSC 215639 had the minimum polar solvation energy and also had a relative strong binding energy. NSC 217026 had the strongest binding energy among other compounds.
Lactic Acidosis Associated with Metformin in Patients with Diabetic Kidney Disease: A Narrative Review
Authors: Rahman Fatkhu , Tuba Syahrul
Published: March 2022

Background: Diabetes Mellitus is a metabolite disorder with parameters of high blood sugar levels. In the management of diabetes can be used the drug metformin is the gold of choice to achieve a therapeutic effect and rarely causes side effects of the drug, but it still has debate view. However, if used in excessive doses for patients with kidney disease, it will be contraindicated with side effects such as lactic acidosis. Objective: This study aims to evaluate the side effect of Metformin for diabetic kidney diseases (DKD) patients. Method: This study used the Narrative Review Method that was obtained from 2011 to 2021, in the English language from PubMed, Google Scholar, and Cochrane Library. Results: Metformin is at the forefront of the treatment of type 2 diabetes mellitus (DM2). Metformin is likely to have lactic acidosis-related adverse effects in chronic kidney disease (CKD) patients, such as increased arterial lactate. Lactic acidosis is defined as an increase in arterial lactate with an indicator of more than five mmol/L and an arterial blood pH of less than 7.35. Metformin-induced lactate levels are considered to be below the parameters. DKD risk factors can be conceptually classified as several susceptibility factors, initiation factors, and developmental factors. The two most prominent risk factors are hyperglycemia and hypertension. Conclusion: Metformin can increase lactate levels in CKD patients but is still below the parameters of lactic acidosis. This study may have some weaknesses and requires further prospective research to validate the results.
Differential Impacts of Climatic and Land Use Changes on Habitat Suitability and Protected Area Adequacy Across the Asian Elephant’s Range
Authors: Yang Wei , Ma Yuanxu , Jing Linhai , Wang Siyuan , Sun Zhongchang , Tang Yunwei , Li Hui
Published: March 2022

The reduction of biodiversity loss is one of the targets of the 2030 Agenda for Sustainable Development. The protection of endangered species is critical for conserving global biodiversity. Asian elephants，as one of the last few mega-herbivores on Earth, are currently threatened by climate changes and anthropogenic modifications. The modelling of their living habitats is of top priority to the conservation of Asian elephant. In this study, we used the maximum entropy model (MaxEnt) to identify the current and potential future habitats of Asian elephants in South and Southeast Asia. We performed analyses for future projections with 17 scenarios by using the present results as baseline. To optimise the modelling results, we delineated the core habitats by using the Core Mapper Tool and compared them with existing protected areas (PAs) through gap analysis. The results showed that the current total area of core habitats is 491,455 km2 in size and will be reduced to 332,544 km2 by 2090 under SSP585 (the shared socioeconomic pathway). The projection analysis under differential scenarios suggested that most of the core habitats in the current protected areas would remain stable and suitable for elephants in the future. However, the remaining 75.17% of the core habitats lay outside the current PAs, and finally we mapped approximately 219,545 km2 of suitable habitats as priority protected areas in the future. Although our model did not perform well in some regions, our analyses and findings still could provide useful references to the planning of protected areas and conservation of Asian elephant.
Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture
Authors: Vázquez Alberdi Lucia , Rosso Gonzalo , Velóz Lucía , Romeo Carlos , Farias Joaquina , Di Tomaso María Vittoria , Calero Miguel , Kun Alejandra
Published: March 2022

Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E is a demyelinating neuropathy affecting Schwann cells due to pmp22 mutations, modelized by Trembler-J mice. Curcumin, a natural polyphenol com-pound obtained from turmeric (Curcuma longa), exhibits dose- and time-varying antitumor, antioxidant and neuroprotective properties, however, the neurotherapeutic actions of curcumin still remain elusive. Here, we propose curcumin as a possible natural treatment capable of enhancing cellular detoxification mechanisms, resulting in an improvement of the neurodegenerative Trembler-J phenotype. Using a refined method for obtaining enriched Schwann cell cultures, we evaluated the neurotherapeutic action of low dose curcumin treatment on the PMP22 expression, and on the chaperones (HSF1 and Hsp27) and autophagy/mTOR (HDAC6 and ribosomes) pathways in Trembler-J and wild-type genotypes. In wild-type Schwann cells, the action of cur-cumin resulted in strong stimulation of the chaperone and macroautophagy pathway, whereas the modulation of ribophagy showed a mild effect. However, despite the promising neuroprotective effects for the treatment of neurological diseases, we report (demonstrate?) that the action of cur-cumin in Trembler-J Schwann cells could be impaired due to the irreversible impact of ethanol used as a common curcumin vehicle necessary for administration. These results contribute to expand our still limited understanding of PMP22 biology in neurobiology, and expose the intrinsic lability of the neurodegenerative Trembler-J genotype. Furthermore, they unravel interesting physiological mechanisms of cellular resilience relevant for to the pharmacological treatment of the neurodegenerative Tremble J phenotype with curcumin and ethanol. We conclude that the analysis of the effects of the vehicle itself is an essential and in-escapable step to comprehensibly assess the own effects and full potential of curcumin treatment for therapeutic purposes.
Radiation-Induced Undifferentiated Spindle Cell Sarcoma Following High-Dose-Rate Interstitial Brachytherapy for Tongue Squamous Cell Carcinoma
Authors: Uchimoto Yuka , Shimamoto Hiroaki , Takeshita Ami , Oya Kaori , Kishino Mitsunobu , Fukuda Yasuo , Toyosawa Satoru , Oda MIchio , Ogawa Kazuhiko , Kreiborg Sven , Mallya Sanjay M. , Murakami Shumei
Published: March 2022

Abstract: High-dose-rate interstitial brachytherapy (HDR-ISBT) has recently come to be considered one of the most effective treatments for oral cancer. On the other hand, it is important to note that radiation therapy has some side effects. Especially, radiation-induced malignancy is probably the most serious complication affecting long-term survivors. We report a case of a radiation-induced undifferentiated spindle cell sarcoma that developed following HDR-ISBT for tongue squamous cell carcinoma (SCC). A 39-year-old woman with right tongue SCC underwent HDR-ISBT (60 Gy, 10 fractions, 8 days) treatment. Five years and one month later, a tumor had developed at the primary site. Surgery was performed for the tumor, which was histopathologically diagnosed as an undifferentiated spindle cell sarcoma. That was distinct from the squamous cell origin of the primary cancer. According to recently established criteria for radiation-induced malignancy, this case was classified as a radiation-induced sarcoma. A search of the literature revealed no previous report of radiation-induced malignancy following HDR-ISBT for tongue cancer.
Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes
Authors: Bogusiewicz Joanna , Kupcewicz Bogumiła , Goryńska Paulina Zofia , Jaroch Karol , Goryński Krzysztof , Birski Marcin , Furtak Jacek , Paczkowski Dariusz , Harat Marek , Bojko Barbara
Published: March 2022

The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method that enables the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology.
Evaluation of Adaptive and Learning in Unmanned Systems
Authors: Koo Sung Mo , Travis Henry D. , Sands Timothy
Published: March 2022

This study determines the threshold for the computational rate of actuator motor controllers for unmanned underwater vehicles necessary to accurately follow discontinuous square wave commands. Motors must track challenging square-wave inputs, and identification of key computational rates permit application of deterministic artificial intelligence (D.A.I.) to achieve tracking to a machine-precision degree of accuracy in direct comparison to other state-of-art approaches. All modeling approaches are validated in MATLAB simulations where the motor process is discretized at varying step-sizes (inversely proportional to computational rate). At a large step-size (fast computational rate), discrete D.A.I. shows a mean error more than three times larger than that of a ubiquitous model-following approach. Yet, at a smaller step size (slower computational rate), the mean error decreases by a factor of 10, only three percent larger than that of continuous D.A.I. Hence, the performance of discrete D.A.I. is critically affected by the sampling period for discretization of the system equations and computational rate. Discrete D.A.I. should be avoided when small step-size discretization is unavailable. In fact, continuous D.A.I. has surpassed all modeling approaches which makes it the safest and most viable solution to future commercial applications in unmanned underwater vehicles.
Comparison of Productivity of Sentul and Kampung Chickens until the Age of 3 Months in the First Generation Selection Population (G1)
Authors: Wahyuni Wahyuni , Depison Depison , Gushariyanto Gushariyanto
Published: February 2022

This study aimed to compare the productivity of Sentul chickens and first-generation Kampung chickens (G1) until the age of 3 months. The research material were Sentul chicken and Kampung chicken. The method used was an experiment method or direct observation with a sample of 174 chickens from each strain. The data collected were egg weight, doc weight, body weight, body weight gain, body measurements, and selection response. Data on egg weight, body weight, and body measurements were analyzed using the average difference test (t-test), while the average value vector of body measurements Sentul chicken and Kampung chicken were analyzed using the T2-Hotelling statistical test. To identify the body size and body shape characteristics of Sentul chickens and Kampung chickens were analyzed using principal component analysis. Data processing used the statistical software Minitab version 18. The results of this study showed that egg weight, body weight at the age of DOC-3 months and body measurements of Sentul chickens were significantly different (P<0.05) higher than the Kampung chickens. This study concludes that the egg weight, body weight, body weight gain, and body sizes of Sentul chickens are higher than Kampung chickens. The size characteristic of Sentul and Kampung chickens is chest circumference. The characteristic of the shape of the Sentul chicken is the length of the wings, while the shape of the native chicken is the width of the chest. The selection response and the heritability value of the Sentul chickens was higher than Kampung chikens.