bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an explainable AI system using routine clinical parameters for rapid differentiation of inflammatory conditions
Authors: Hoffmann Joerg , Rheude Anne , Neubauer Andreas , Brendel Cornelia , Thrun Michael C.
Published: March 2024

Introduction

Inflammatory conditions in patients have various causes and require different treatments. Bacterial infections are treated with antibiotics, while these medications are ineffective against viral infections. Autoimmune diseases and graft-versus-host disease (GVHD) after allogeneic stem cell transplantation, require immunosuppressive therapies such as glucocorticoids, which may be contraindicated in other inflammatory states. In this study, we employ a combination of straightforward blood tests to devise an explainable artificial intelligence (XAI) for distinguishing between bacterial infections, viral infections, and autoimmune diseases/graft-versus-host disease.

Patients and methods

We analysed peripheral blood from 80 patients with inflammatory conditions and 38 controls. Complete blood count, CRP analysis, and a rapid flow cytometric test for myeloid activation markers CD169, CD64, and HLA-DR were utilized. A two-step XAI distinguished firstly with C5.0 rules pruned by ABC analysis between controls and inflammatory conditions and secondly between the types of inflammatory conditions with a new bivariate decision tree using the Simpson impurity function.

Results

Inflammatory conditions were distinguished using an XAI, achieving an overall accuracy of 81.0% (95%CI 72 – 87%). Bacterial infection (N = 30), viral infection (N = 26), and autoimmune diseases/GVHD (N = 24) were differentiated with accuracies of 90.3%, 80.0%, and 79.0%, respectively. The most critical parameter for distinguishing between controls and inflammatory conditions was the expression of CD64 on neutrophils. Monocyte count and expression of CD169 were most crucial for the classification within the inflammatory conditions.

Conclusion

Treatment decisions for inflammatory conditions can be effectively guided by XAI rules, straightforward to implement and based on promptly acquired blood parameters.
Targeting staphylococcal enterotoxin B binding to CD28 as a new strategy for dampening superantigen-mediated intestinal epithelial barrier dysfunctions
Authors: Amormino Carola , Russo Emanuela , Tedeschi Valentina , Fiorillo Maria Teresa , Paiardini Alessandro , Spallotta Francesco , Rosanò Laura , Tuosto Loretta , Kunkl Martina
Published: March 2024

Staphylococcus aureus is a gram-positive bacterium that may cause intestinal inflammation by secreting enterotoxins, which commonly cause food-poisoning and gastrointestinal injuries. Staphylococcal enterotoxin B (SEB) acts as a superantigen (SAg) by binding in a bivalent manner the T-cell receptor (TCR) and the costimulatory receptor CD28, thus stimulating T cells to produce large amounts of inflammatory cytokines, which may affect intestinal epithelial barrier integrity and functions. However, the role of T cell-mediated SEB inflammatory activity remains unknown. Here we show that inflammatory cytokines produced by T cells following SEB stimulation induce dysfunctions in Caco-2 intestinal epithelial cells by promoting actin cytoskeleton remodelling and epithelial cell-cell junction down-regulation. We also found that SEB-activated inflammatory T cells promote the up-regulation of epithelial-mesenchymal transition transcription factors (EMT-TFs) in a nuclear factor-κB (NF-κB)- and STAT3-dependent manner. Finally, by using a structure-based design approach, we identified a SEB mimetic peptide (pSEB116-132) that, by blocking the binding of SEB to CD28, dampens inflammatory-mediated dysregulation of intestinal epithelial barrier.
Self-supervised learning for Formosan speech representation and linguistic phylogeny
Authors: Hsieh Shu-Kai , Tseng Yu-Hsiang , Lian Da-Chen , Wang Chi-Wei
Published: March 2024

Formosan languages, spoken by the indigenous peoples of Taiwan, have unique roles in the reconstruction of Proto-Austronesian Languages. This paper presents a real-world Formosan language speech dataset, including 144 h of news footage for 16 Formosan languages, and uses self-supervised models to obtain and analyze their speech representations. Among the news footage, 13 h of the validated speech data of Formosan languages are selected, and a language classifier, based on XLSR-53, is trained to classify the 16 Formosan languages with an accuracy of 86%. We extracted and analyzed the speech vector representations learned from the model and compared them with 152 manually coded linguistic typological features. The comparison shows that the speech vectors reflect Formosan languages' phonological and morphological aspects. Furthermore, the speech vectors and linguistic features are used to construct a linguistic phylogeny, and the resulting genealogical grouping corresponds with previous literature. These results suggest that we can investigate the current real-world language usages through the speech model, and the dataset opens a window to look into the Formosan languages in vivo.
Long-term reduced functional capacity and quality of life in hospitalized COVID-19 patients
Authors: da Silveira Anderson Donelli , Scolari Fernando Luis , Saadi Marina Petersen , Brahmbhatt Darshan H. , Milani Mauricio , Milani Juliana Goulart Prata Oliveira , Junior Gerson Cipriano , Sartor Ivaine Tais Sauthier , Zavaglia Gabriela Oliveira , Tonini Maiko Luis , da Costa Marcela Santos Correa , Scotta Marcelo Comerlato , Stein Renato T. , Rosa Regis Goulart
Published: March 2024

Background

Persistent symptoms and exercise intolerance have been reported after COVID-19, even months after the acute disease. Although, the long-term impact on exercise capacity and health-related quality of life (HRQoL) is still unclear.

Research question

To assess the long-term functional capacity and HRQoL in patients hospitalized due to COVID-19.

Study design and methods

This is a prospective cohort study, conducted at two centers in Brazil, that included post-discharge COVID-19 patients and paired controls. The cohort was paired by age, sex, body mass index and comorbidities, using propensity score matching in a 1:3 ratio. Patients were eligible if signs or symptoms suggestive of COVID-19 and pulmonary involvement on chest computed tomography. All patients underwent cardiopulmonary exercise testing (CPET) and a HRQoL questionnaire (SF-36) 6 months after the COVID-19. The main outcome was the percentage of predicted peak oxygen consumption (ppVO2). Secondary outcomes included other CPET measures and HRQoL.

Results

The study sample comprised 47 post-discharge COVID-19 patients and 141 healthy controls. The mean age of COVID-19 patients was 54 ± 14 years, with 19 (40%) females, and a mean body mass index of 31 kg/m2 (SD, 6). The median follow-up was 7 months (IQR, 6.5–8.0) after hospital discharge. PpVO2 in COVID-19 patients was lower than in controls (83% vs. 95%, p = 0.002) with an effect size of 0.38 ([95%CI], 0.04–0.70). Mean peak VO2 (22 vs. 25 mL/kg/min, p = 0.04) and OUES (2,122 vs. 2,380, p = 0.027) were also reduced in the COVID-19 patients in comparison to controls. Dysfunctional breathing (DB) was present in 51%. HRQoL was significantly reduced in post COVID patients and positively correlated to peak exercise capacity.

Interpretation

Hospitalized COVID-19 patients presented, 7 months after discharge, with a reduction in functional capacity and HRQoL when compared to historical controls. HRQoL were reduced and correlated with the reduced peak VO2 in our population.
Safety, tolerability, and efficacy estimate of evoked gamma oscillation in mild to moderate Alzheimer’s disease
Authors: Hajós Mihály , Boasso Alyssa , Hempel Evan , Shpokayte Monika , Konisky Alex , Seshagiri Chandran V. , Fomenko Vitella , Kwan Kim , Nicodemus-Johnson Jessie , Hendrix Suzanne , Vaughan Brent , Kern Ralph , Megerian Jonathan T. , Malchano Zach
Published: March 2024

Background

Alzheimer’s Disease (AD) is a multifactorial, progressive neurodegenerative disease that disrupts synaptic and neuronal activity and network oscillations. It is characterized by neuronal loss, brain atrophy and a decline in cognitive and functional abilities. Cognito’s Evoked Gamma Therapy System provides an innovative approach for AD by inducing EEG-verified gamma oscillations through sensory stimulation. Prior research has shown promising disease-modifying effects in experimental AD models. The present study (NCT03556280: OVERTURE) evaluated the feasibly, safety and efficacy of evoked gamma oscillation treatment using Cognito’s medical device (CogTx-001) in participants with mild to moderate AD.

Methods

The present study was a randomized, double blind, sham-controlled, 6-months clinical trial in participants with mild to moderate AD. The trial enrolled 76 participants, aged 50 or older, who met the clinical criteria for AD with baseline MMSE scores between 14 and 26. Participants were randomly assigned 2:1 to receive self-administered daily, one-hour, therapy, evoking EEG-verified gamma oscillations or sham treatment. The CogTx-001 device was use at home with the help of a care partner, over 6 months. The primary outcome measures were safety, evaluated by physical and neurological exams and monthly assessments of adverse events (AEs) and MRI, and tolerability, measured by device use. Although the trial was not statistically powered to evaluate potential efficacy outcomes, primary and secondary clinical outcome measures included several cognitive and functional endpoints.

Results

Total AEs were similar between groups, there were no unexpected serious treatment related AEs, and no serious treatment-emergent AEs that led to study discontinuation. MRI did not show Amyloid-Related Imaging Abnormalities (ARIA) in any study participant. High adherence rates (85–90%) were observed in sham and treatment participants. There was no statistical separation between active and sham arm participants in primary outcome measure of MADCOMS or secondary outcome measure of CDR-SB or ADAS-Cog14. However, some secondary outcome measures including ADCS-ADL, MMSE, and MRI whole brain volume demonstrated reduced progression in active compared to sham treated participants, that achieved nominal significance.

Conclusion

Our results demonstrate that 1-h daily treatment with Cognito’s Evoked Gamma Therapy System (CogTx-001) was safe and well-tolerated and demonstrated potential clinical benefits in mild to moderate AD.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT03556280.
Resting-state functional MRI study of conventional MRI-negative intractable epilepsy in children
Authors: Li Xuhong , Liu Heng , Zhang Tijiang
Published: March 2024

Objective

The study aimed at investigating functional connectivity strength (FCS) changes in children with MRI-negative intractable epilepsy (ITE) and evaluating correlations between aberrant FCS and both disease duration and intelligence quotient (IQ).

Methods

Fifteen children with ITE, 24 children with non-intractable epilepsy (nITE) and 25 matched healthy controls (HCs) were subjected to rs-fMRI. IQ was evaluated by neuropsychological assessment. Voxelwise analysis of covariance was conducted in the whole brain, and then pairwise comparisons were made across three groups using Bonferroni corrections.

Results

FCS was significantly different among three groups. Relative to HCs, ITE patients exhibited decreased FCS in right temporal pole of the superior temporal gyrus, middle temporal gyrus, bilateral precuneus, etc and increased FCS values in left triangular part of the inferior frontal gyrus, parahippocampal gyrus, supplementary motor area, caudate and right calcarine fissure and surrounding cortex and midbrain. The nITE patients presented decreased FCS in right orbital superior frontal gyrus, precuneus etc and increased FCS in bilateral fusiform gyri, parahippocampal gyri, etc. In comparison to nITE patients, the ITE patients presented decreased FCS in right medial superior frontal gyrus and left inferior temporal gyrus and increased FCS in right middle temporal gyrus, inferior temporal gyrus and calcarine fissure and surrounding cortex. Correlation analysis indicated that FCS in left caudate demonstrated correlation with verbal IQ (VIQ) and disease duration.

Conclusion

ITE patients demonstrated changed FCS values in the temporal and prefrontal cortices relative to nITE patients, which may be related to drug resistance in epilepsy. FCS in the left caudate nucleus associated with VIQ, suggesting the caudate may become a key target for improving cognitive impairment and seizures in children with ITE.
Combinatorial strategies for cell transplantation in traumatic spinal cord injury
Authors: Jagrit Vipin , Koffler Jacob , Dulin Jennifer N.
Published: March 2024

Spinal cord injury (SCI) substantially reduces the quality of life of affected individuals. Recovery of function is therefore a primary concern of the patient population and a primary goal for therapeutic interventions. Currently, even with growing numbers of clinical trials, there are still no effective treatments that can improve neurological outcomes after SCI. A large body of work has demonstrated that transplantation of neural stem/progenitor cells (NSPCs) can promote regeneration of the injured spinal cord by providing new neurons that can integrate into injured host neural circuitry. Despite these promising findings, the degree of functional recovery observed after NSPC transplantation remains modest. It is evident that treatment of such a complex injury cannot be addressed with a single therapeutic approach. In this mini-review, we discuss combinatorial strategies that can be used along with NSPC transplantation to promote spinal cord regeneration. We begin by introducing bioengineering and neuromodulatory approaches, and highlight promising work using these strategies in integration with NSPCs transplantation. The future of NSPC transplantation will likely include a multi-factorial approach, combining stem cells with biomaterials and/or neuromodulation as a promising treatment for SCI.
Dietary survey of poultry egg intake among residents in Kunming city, southwest China
Authors: Wang Rui , Wu Yugao , Zhang Chenxi , Liang Chengyao , Xia Xiran , Gao Ximan , Fang Jing
Published: March 2024

Background

For almost all people, eggs can be a wholesome addition to the diet. However, there is insufficient applicable data to evaluate the poultry egg intake of people in the city of Kunming located in southwest China.

Objectives

To understand the situation of egg consumption among local residents in Kunming via a dietary survey.

Methods

Residents living in three places of Kunming were chosen using a multi-stage random sampling method. The recall methods of 3-day food intake and 1-month food intake frequency were used to assess the quantity and frequency of poultry egg dietary intake of local people.

Results

Of the 1,118 respondents, 565 (50.54%) were female and 553 (49.46%) were male with age range 0.5–91 years old. Egg consumption was widespread among the survey respondents with 88.01% reporting hen egg ingestion, but the dietary intake of other poultry eggs such as duck, quail, and goose eggs were much less frequent. The medium daily intake of hen eggs was 20.00 g/d with greater amount of hen egg consumption in older age groups. However, when calculated on a body-weight basis, the median amount of hen eggs consumed daily per kilogram of body weight for all survey respondents was 0.47 g/kg/d whereas this indicator for children was 1.33 g/kg/d, becoming the greatest among all age groups.

Conclusions

Our study obtained a better understanding of poultry egg intake among residents in Kunming city and calculated the egg intake kilogram of body weight that can be a useful reference to inform the development of more accurate dietary recommendation. These results also provide basic data for nutrition monitoring and dietary exposure risk assessment of poultry egg intake.
Criteria of ultrasound-guided closed reduction with percutaneous pinning in unstable humeral lateral condylar fractures: a three-center retrospective cohort study
Authors: Huang Xiuming , Liu Chaoyu , Jia Guoqiang , Yao Jie , Xu Jianbing
Published: March 2024

Background

Interventions using ultrasound-guided closed reduction and percutaneous pinning (UG-CRPP) of humeral lateral condylar fractures (HLCFs) have been increasingly applied; however, their effectiveness for unstable HLCFs and the criteria for ultrasound outcomes remain unclear. This study assessed the outcomes of UG-CRPP for HLCFs and evaluated the success criteria in children.

Methods

Data were retrospectively collected from 106 patients with unstable HLCFs admitted to three hospitals between January 2021 and August 2022. Fifty-five cases were left-sided and 51 cases were right-sided: 74 male patients and 32 female patients were included. Perioperative data, elbow function, complications, and criteria for UG-CRPP were analyzed.

Results

The mean rate of UG-CRPP was 88%. The mean surgical time was 54.56 ± 21.07 min, and the mean fluoroscopy frequency was 9.25 ± 2.93 times. At the last follow-up, there were significant differences in elbow flexion between the affected side (135.82° ± 6.92°) and the unaffected side (140.58° ± 5.85°) (p = 0.01). The Mayo score of the affected side was 90.28° ± 4.97°, the Baumann angle was 71.4° ± 5.4°, condylar shaft angle was 39.9° ± 6.4°, and the carrying angle was 8.4° ± 3.6°. Seventy patients presented mild lateral spurs and 16 patients exhibited moderate spurs. Fourteen patients presented with pin infection, and one patient exhibited postoperative re-displacement. There was no premature physeal closure, varus, or valgus elbow deformity, delayed union, or non-union. Successful ultrasound-based outcome criteria for UG-CRPP were defined as follows: (i) absent or less than a cartilage thickness step on the cartilage hinge on coronal plane parallel articular surface scanning, (ii) no lateral displacement and intact distal end of the condylar and capitellum on coronal plane vertical articular surface scanning, (iii) no anteroposterior displacement and absent or less than a cartilage thickness step on sagittal plane vertical articular surface scanning, and (iv) intact posterior fracture line or less than a cortex step on posterolateral sagittal plane vertical articular surface scanning.

Conclusion

UG-CRPP is a procedure with minimal blood loss, less invasive, cosmetic, and no radiation exposure. It yielded good outcomes in unstable HLCFs. The successful criteria make it suitable for clinical application.
Songwriting Group Music Therapy to promote psychological adjustment in informal caregivers of elderly people with dependency: a mixed methods study
Authors: Pérez-Núñez Paula , O’Callaghan Clare , López-Paz Juan Francisco , Ruiz de Lazcano Aitana , Rodríguez Alicia Aurora , Amayra Imanol
Published: March 2024

Introduction

Informal caregivers of elderly people with dependency (EPD) provide intensive care that can affect their quality of life (QoL). Psychosocial interventions such as music therapy are important to work on their self-care. The aim of this study is to analyze, with a mixed method approach, the experience of participating in a Songwriting Group Music Therapy (SGMT) intervention on informal caregivers of EPD.

Methods

A total of 11 groups, with a convenience sample of 61 caregivers, received 10 SGMT sessions. Quantitative information related to QoL variables (anxiety, depression, spirituality, burden, and coping) was collected before and after the intervention and at 3 months of follow-up. Regarding qualitative data, an open-ended question about the experience of participating was asked.

Results

Significant changes were shown, sustained over time, in trait anxiety and depression and subscales including inner peace, social functioning, and mental health. Three themes were generated from the thematic analysis, including that SGMT participation can enhance personal growth, bring out and enable work on emotions, and promote helpful interpersonal dynamics.

Discussion

The findings indicate that SGMT is a useful intervention for informal caregivers of EPD, promoting psychological adjustment, enhanced coping, emotional regulation, and social support. This study reinforces the findings with caregivers of other populations, providing new results and highlighting the benefits of SGMT for caregivers of EPD.
Fronto-striato network function is reduced in major depressive disorder
Authors: Kijima Reoto , Watanabe Keita , Okamoto Naomichi , Ikenouchi Atsuko , Tesen Hirofumi , Kakeda Shingo , Yoshimura Reiji
Published: March 2024

Introduction

Major depressive disorder (MDD) is a major cause of poor quality of life and disability and is highly prevalent worldwide. Various pathological mechanisms are implicated in MDD, including the reward system. The human brain is equipped with a reward system that is involved in aspects such as motivation, pleasure, and learning. Several studies including a meta-analysis have been reported on the reward system network and MDD. However, to our knowledge, no studies have examined the relationship between the reward system network of drug-naïve, first-episode MDD patients and the detailed symptoms of MDD or age. The fronto-striato network (FSN) is closely related to the reward system network. The present study primarily aimed to elucidate this point.

Methods

A total of 89 drug-naïve first-episode MDD patients and 82 healthy controls (HCs) patients were enrolled in the study. The correlation between the FSN and age and the interaction between age and illness in the FSN were investigated in 75 patients in the MDD group and 79 patients in the HC group with available information on the FSN and age. In addition, the association between the FSN and the total scores on the 17-item Hamilton Rating Scale for Depression (HAMD-17) and scores in each symptom item was analyzed in 76 MDD subjects with information on the FSN and HAMD-17. The significance of each result was evaluated according to a p-value of <0.05.

Results

Age was inversely correlated with the FSN (p=2.14e-11) in the HC group but not in the MDD group (p=0.79). FSN varied with the presence of MDD and with age, particularly showing an interaction with MDD and age (p=1.04e-08). Specifically, age and the presence or absence of MDD each affected FSN, but the effect of age on FSN changed in the presence of depression. FSN did not correlate with total HAMD-17 scores or scores in each item.

Discussion

The reward system may be dysfunctional in patients with MDD. In addition, the effect could be greater in younger patients. Meanwhile, there is no correlation between the function of the reward system and the severity of MDD or the severity of each symptom. Thus, the reward system network may be an important biological marker of MDD, although careful consideration should be given to age and its association with the severity of the disorder.

Conclusion

The reward system function is decreased in MDD patients, and this decrease may be more pronounced in younger patients, although further research is still needed.
Temporal dynamic effects of meteorological factors and air quality on the physical health of the older adults in Shenzhen, China
Authors: Jiang Shuai , Han Chuanliang , Ma Yue , Ji Jiajia , Chen Guomin , Guo Yinsheng
Published: March 2024

Introduction

Meteorological and environmental factors can affect people’s lives and health, which is crucial among the older adults. However, it is currently unclear how they specifically affect the physical condition of older adults people.

Methods

We collected and analyzed the basic physical examination indicators of 41 older adults people for two consecutive years (2021 and 2022), and correlated them with meteorological and environmental factors. Partial correlation was also conducted to exclude unrelated factors as well.

Results

We found that among the physical examination indicators of the older adults for two consecutive years, five indicators (HB, WBC, HbAlc, CB, LDL-C) showed significant differences across the population, and they had significantly different dynamic correlation patterns with six meteorological (air pressure, temperature, humidity, precipitation, wind speed, and sunshine duration) and seven air quality factors (NO2, SO2, PM10, O3-1h, O3-8h, CO, PM2.5).

Discussion

Our study has discovered for the first time the dynamic correlation between indicators in normal basic physical examinations and meteorological factors and air quality indicators, which will provide guidance for the future development of policies that care for the healthy life of the older adults.
Preliminary evaluation of a novel group-based motivational interviewing intervention with adolescents: a feasibility study
Authors: Vallentin-Holbech Lotte , Karsberg Sidsel Helena , Nielsen Anette Søgaard , Feldstein Ewing Sarah W. , Rømer Thomsen Kristine
Published: March 2024

Drinking is closely intertwined with social life among many adolescents, particularly in Europe. Group-based interventions, such as group-based motivational interviewing (group MI), have shown the capacity to prevent and reduce hazardous drinking and related problems among adolescents, but few examinations have been conducted in a European high school setting. This study examines the preliminary outcomes of a pilot group MI intervention among Danish adolescents. High school students (ages 15–18 years) were randomly allocated to two 1-h group MI sessions delivered in a school setting (N = 65) or an assessment only control condition (N = 67). Data were collected in August and November 2020 using online self-administrated questionnaires regarding the acceptability of the intervention and past month alcohol use. The pilot group MI intervention showed high feasibility and acceptability in this setting and with this age group. Group MI adolescents significantly reduced peak drinks per drinking day compared to assessment only adolescents (−2.7 drinks, p < 0.05). Results are discussed in relation to the metrics being evaluated during COVID-19 lockdown, including increased social restrictions at follow-up compared to baseline. Group MI shows promise for reducing hazardous alcohol use among Danish adolescents. In addition, the findings indicate the importance of building on and extending this work in future larger, better-powered randomized controlled trials.
The ethics of clinical research in the era of COVID-19
Authors: Vitti Juliana N. , Vitti Robert , Chu Karen , Mellis Scott
Published: March 2024

There is an urgent need for increased understanding of COVID-19 and strategies for its prevention, treatment, and mitigation. All participants in the research enterprise, including institutional review boards, have an ethical duty to protect participants and ensure that the benefits gained from such research do not conflict with the core principles that guided researchers prior to the pandemic. In this review, we discuss the ethical issues surrounding initiation and conduct of clinical trials, focusing on novel COVID-19 therapeutic, vaccine, or biospecimen research, using the principles of autonomy, beneficence, and justice. We discuss strategies to manage the practical challenges associated with the conduct of clinical trials, with an emphasis on maintaining the rights and welfare of research participants.
The research trends of ferroptosis in diabetes: a bibliometric analysis
Authors: Xiong Liyuan , Hu Faquan , Li Zhengpin , Zhou Xuemei , Zheng Yujiao
Published: March 2024

Objective

Exploring the mechanism of ferroptosis as a potential avenue for investigating the pathogenesis and therapeutic outlook of diabetes mellitus and its complications has emerged as a focal point within recent years. Herein, we employ a bibliometric approach to delineate the current landscape of ferroptosis research in the context of diabetes mellitus. Our objective is to furnish insights and scholarly references conducive to the advancement of comprehensive investigations and innovations in related domains.

Methods

We included studies on ferroptosis in diabetes, obtained from the Web of Science Core Collection. All publications were transported in plaintext full-record format and were analyzed by CiteSpace 6.2.R4 for bibliometric analysis.

Results

Four hundred and forty-eight records that met the criteria were included. The publications released during the initial 3 years were relatively small, while there was a sudden surge of publications published in 2022 and 2023. Representing 41 countries and 173 institutions, China and Wuhan University led the research on ferroptosis in diabetes. The author with the highest number of published papers is Zhongming Wu, while Dixon SJ is the most frequently cited author. The journal with the highest number of co-citations is Cell. The most common keywords include oxidative stress, cell death, lipid peroxidation, and metabolism. Extracted keywords predominantly focus on NLRP3 inflammatory, diabetic kidney disease, mitochondria, iron overload, and cardiomyopathy.

Conclusion

The escalating recognition of ferroptosis as a potential therapeutic target for deciphering the intricate mechanisms underlying diabetes and its complications is underscored by a noteworthy surge in relevant research publications. This surge has catapulted ferroptosis into the spotlight as a burgeoning and vibrant research focus within the field.
Spatial pattern of isoniazid-resistant tuberculosis and its associated factors among a population with migrants in China: a retrospective population-based study
Authors: Zhang Hongyin , Sun Ruoyao , Wu Zheyuan , Liu Yueting , Chen Meiru , Huang Jinrong , Lv Yixiao , Zhao Fei , Zhang Yangyi , Li Minjuan , Jiang Hongbing , Zhan Yiqiang , Xu Jimin , Xu Yanzi , Yuan Jianhui , Zhao Yang , Shen Xin , Yang Chongguang
Published: March 2024

Background

Isoniazid-resistant, rifampicin-susceptible tuberculosis (Hr-TB) globally exhibits a high prevalence and serves as a potential precursor to multidrug-resistant tuberculosis (MDR-TB). Recognizing the spatial distribution of Hr-TB and identifying associated factors can provide strategic entry points for interventions aimed at early detection of Hr-TB and prevention of its progression to MDR-TB. This study aims to analyze spatial patterns and identify socioeconomic, demographic, and healthcare factors associated with Hr-TB in Shanghai at the county level.

Method

We conducted a retrospective study utilizing data from TB patients with available Drug Susceptible Test (DST) results in Shanghai from 2010 to 2016. Spatial autocorrelation was explored using Global Moran’s I and Getis-Ord Gi∗ statistics. A Bayesian hierarchical model with spatial effects was developed using the INLA package in R software to identify potential factors associated with Hr-TB at the county level.

Results

A total of 8,865 TB patients with DST were included in this analysis. Among 758 Hr-TB patients, 622 (82.06%) were new cases without any previous treatment history. The drug-resistant rate of Hr-TB among new TB cases in Shanghai stood at 7.20% (622/8014), while for previously treated cases, the rate was 15.98% (136/851). Hotspot areas of Hr-TB were predominantly situated in southwestern Shanghai. Factors positively associated with Hr-TB included the percentage of older adult individuals (RR = 3.93, 95% Crl:1.93–8.03), the percentage of internal migrants (RR = 1.35, 95% Crl:1.15–1.35), and the number of healthcare institutions per 100 population (RR = 1.17, 95% Crl:1.02–1.34).

Conclusion

We observed a spatial heterogeneity of Hr-TB in Shanghai, with hotspots in the Songjiang and Minhang districts. Based on the results of the models, the internal migrant population and older adult individuals in Shanghai may be contributing factors to the emergence of areas with high Hr-TB notification rates. Given these insights, we advocate for targeted interventions, especially in identified high-risk hotspots and high-risk areas.
Use of an upright power wheelchair in spinal cord injury: a case series
Authors: Hong Eunkyoung , Elliott Michael , Kornfeld Stephen , Spungen Ann M.
Published: March 2024

Objective

To explore independence, usability, and self-reported quality of life (QOL) in eligible persons with spinal cord injury (SCI) who used a standing powered wheelchair over a 12-week period. Setting: VA SCI research facility.

Participants

Four participants with chronic SCI who use a wheelchair as the primary means of mobility.

Intervention

A standing power wheelchair was used three times a week (3.5 h/session) for 12 weeks in a supervised setting. Main Outcome Measures: safety, usability and feasibility, blood pressure in seated and standing positions, bowel, bladder, and pain item banks from the SCI-QOL Physical-Medical-Health domain, and overall user satisfaction with the device.

Results

Participants consistently maintained normal blood pressure responses between seated and standing positions throughout the training sessions and learned to perform all the mobility tasks safely and independently. Participants reported improvements on the SCI-QOL and were generally satisfied with the upright standing power wheelchair.

Conclusions

In this small case series of chronic, non-ambulatory individuals with SCI, the standing powered wheelchair was shown to be safe and efficacious.
Obstructive sleep apnea mouth breathing phenotype response to combination oral appliance therapy
Authors: Schramm Preetam , Schneiderman Emet , Hui Jason , German Zohre , Stenberg William , Lin Ju Ying
Published: March 2024

Introduction

Obstructive sleep apnea (OSA) is a multisystem physiological disorder of breathing during sleep that may contribute to systemic physiological imbalances and can also be exacerbated by the use of some commonly prescribed medications.

Methods

In a randomized parallel design trial, we included phenotypic mild to severe OSA mouth-breathing subjects (n = 36) confirmed by home polygraphy, to evaluate the efficacy of oral appliance plus mouth shield and oral appliance only during sleep on night 1 (T1) after 4 weeks (T2), and after 8 weeks (T3) of oral appliance therapy. Respiratory dynamics data were collected. Primary outcomes were respiratory event index and mouth breathing. Anamnesis on medication intake was collected at enrollment.

Results

The respiratory event index and the hypopnea index did not statistically differ between groups at T3. Oral appliance plus mouth shield and oral appliance only significantly reduced mouth breathing at T2 (p = 0.012) and T3 (p ≤ 0.001) compared with baseline. Exploratory analyses showed oral appliance plus mouth shield supine respiratory rate at T3 (p = 0.039) was marginally decreased compared with oral appliance only. The snore percentage did not differ statistically between groups at T3. Oral appliance only showed a marginal oxygen saturation increase (p = 0.019) at T3 compared with oral appliance plus mouth shield. At T3, medication users had persistent respiratory events, mouth breathing, and snoring compared with non-medication users. Logistic regression showed medication use may increase the odds of mouth breathing (OR = 1.148; p = 0.015) and snoring (OR = 1.036; p = 0.049).

Discussion

In our OSA-mouth breathing cohort, oral appliance only was similar to oral appliance plus mouth shield in attenuating the respiratory event index, hypopnea index, and mouth breathing after 8 weeks. Oral appliance only increased oxygen saturation at T3, while oral appliance plus mouth shield maintained a relatively narrow oxygen saturation range from T1–3. Oral appliance plus mouth shield marginally lowered the supine respiratory rate at T3 compared with oral appliance only. Persistent respiratory events, mouth breathing, and snoring were observed in medication users at T3.
What Can Happen When Brain Cells Communicate Improperly?
Authors: Maltseva Margarita , Klotz Kerstin Alexandra , Jacobs Julia
Published: March 2024

Nerve cells, also called neurons, behave like tiny messengers in our bodies that help us sense and move. When brain cells chat with each other, it results in electrical brain waves. Sometimes brain cells chat with each other in a calm and slow way, while other times they are excited and brain activity is faster. This fast electrical activity is called oscillations. Equipment can be used to measure the electrical activity in the brain. The fastest activity that can be measured is called high frequency oscillations (HFOs). Fast brain activity can be super helpful in daily life, helping us to do things like memorize locations and activities, for example. However, if neurons start firing too fast, people can experience a sudden loss of control of certain body parts or even the whole body, which is called epilepsy. In this article, you will learn about brain function and epilepsy and how scientists count the speed of brain waves. So, let us have a look at how HFOs help our brains to function.
Bees Could Visit Flower “Pharmacies” When They Are Sick
Authors: Gekière Antoine , Vanderplanck Maryse
Published: March 2024

When you are sick, you might go to the pharmacy to get some medicines. But have you ever wondered what do animals do when they are sick? Bees are tremendously important given the roles they play in the environment and human societies. Unfortunately, bees have been declining in the last decades, partly due to infection by parasites. But certain flowers may help them! Bees collect resources from various flowers and, recently, scientists have shown that some of these flower resources could help bees fight infections. Flower resources contain many essential nutrients as well as very specific medicines that could strengthen the bees or kill the parasites. Once sick, bees could prioritize the collection of resources from specific flowers to deal with their parasites and heal themselves. It is therefore important for us to plant flowers rich in essential nutrients, as well as flowers rich in medicines that help bees to thrive and fight off infections.
In vitro and in vivo studies on the effect of a mitochondrial fusion promoter on Leydig cell integrity and function
Authors: Garza Samuel , Sottas Chantal , Gukasyan Hovhannes J. , Papadopoulos Vassilios
Published: March 2024

Background: The interstitial testicular Leydig cells are responsible for the production of testosterone, which functionally deteriorate with normal aging. Decreased expression of mitochondrial steroidogenic interactome proteins and diminished mitochondrial function in aging Leydig cells suggest that mitochondrial dynamics play a role in maintaining adequate levels of testosterone. Optic atrophy 1 (OPA1) protein regulates mitochondrial dynamics and cristae formation in many cell types. Previous studies showed that increasing OPA1 expression in dysfunctional Leydig cells restored mitochondrial function and recovered androgen production to levels found in healthy Leydig cells. These findings suggested that mitochondrial dynamics may be a promising target to ameliorate diminished testosterone levels in aging males.

Methods: We used twelve-month-old rats to explore the relationship between mitochondrial dynamics and Leydig cell function. Isolated Leydig cells from aged rats were treated ex vivo with the cell-permeable mitochondrial fusion promoter 4-Chloro-2-(1-(2-(2,4,6-trichlorophenyl)hydrazono)ethyl) phenol (mitochondrial fusion promoter M1), which enhances mitochondrial tubular network formation. In parallel, rats were treated with 2 mg/kg/day M1 for 6 weeks before Leydig cells were isolated.

Results:Ex vivo M1-treated cells showed enhanced mitochondrial tubular network formation by transmission electron microscopy, enhanced Leydig cell mitochondrial integrity, improved mitochondrial function, and higher testosterone biosynthesis compared to controls. However, in vivo treatment of aged rats with M1 not only failed to re-establish testosterone levels to that of young rats, it also led to further reduction of testosterone levels and increased apoptosis, suggesting M1 toxicity in the testis. The in vivo M1 toxicity seemed to be tissue-specific, however.

Conclusion: Promoting mitochondrial fusion may be one approach to enhancing cell health and wellbeing with aging, but more investigations are warranted. Our findings suggest that fusion promoters could potentially enhance the productivity of aged Leydig cells when carefully regulated.
Few-Shot Fine-Grained Image Classification: A Comprehensive Review
Authors: Ren Jie , Li Changmiao , An Yaohui , Zhang Weichuan , Sun Changming
Published: March 2024

Few-shot fine-grained image classification (FSFGIC) methods refer to the classification of images (e.g., birds, flowers, and airplanes) belonging to different subclasses of the same species by a small number of labeled samples. Through feature representation learning, FSFGIC methods can make better use of limited sample information, learn more discriminative feature representations, greatly improve the classification accuracy and generalization ability, and thus achieve better results in FSFGIC tasks. In this paper, starting from the definition of FSFGIC, a taxonomy of feature representation learning for FSFGIC is proposed. According to this taxonomy, we discuss key issues on FSFGIC (including data augmentation, local and/or global deep feature representation learning, class representation learning, and task-specific feature representation learning). In addition, the existing popular datasets, current challenges and future development trends of feature representation learning on FSFGIC are also described.
Anomaly Detection and Identification Method for Shield Tunneling Based on Energy Consumption Perspective
Authors: Hu Min , Zhang Fan , Wu Huiming
Published: March 2024

Various abnormal scenarios might occur during the shield tunneling process, which have an impact on construction efficiency and safety. Existing research on shield tunneling construction anomaly detection typically designs models based on the characteristics of a specific anomaly, so the scenarios of anomalies that can be detected are limited. Therefore, the research objective of this article is to establish an accurate anomaly detection model with generalization and identification capabilities on multiple types of abnormal scenarios. Inspired by energy dissipation theory, this paper innovatively detects various anomalies in the shield tunneling process from the perspective of energy consumption and designs the AD_SI model (Anomaly Detection and Scenario Identification model of shield tunneling) based on machine learning. The AD_SI model first monitors the shield machine’s energy consumption status based on the VAE-LSTM (Variational Autoencoder–Long Short-Term Memory) algorithm with a dynamic threshold, thereby detecting abnormal sections. Secondly, the AD_SI model uses the correlation of construction parameters to represent different known scenarios and further clarifies scenarios of the abnormal sections, thus achieving anomaly identification. The application of the AD_SI model in a shield tunneling construction project demonstrates its capability to accurately detect and identify different anomalies, with a recall value exceeding 0.9 and F1 exceeding 0.8, thereby providing guidance for accurately detecting multiple types anomaly scenarios in practical applications.
Association between the Static and Dynamic Lung Function and CT-Derived Thoracic Skeletal Muscle Measurements–A Retrospective Analysis of a 12-Month Observational Follow-Up Pilot Study
Authors: Brath Mia , Alsted Sisse , Sahakyan Marina , Mark Esben , Frøkjær Jens , Rasmussen Henrik , Østergaard Lasse , Christensen Rasmus , Weinreich Ulla
Published: March 2024

Background: Patients with chronic obstructive pulmonary disease (COPD) with low skeletal muscle mass and severe airway obstruction have higher mortality risks. However, the relationship between dynamic/static lung function (LF) and thoracic skeletal muscle measurements (SMM) remains unclear. This study explored patient characteristics (weight, BMI, exacerbations, dynamic/static LF, sex differences in LF and SMM, and the link between LF and SMM changes. Methods: A retrospective analysis of a 12-month prospective follow-up study patients with stable COPD undergoing standardized treatment, covering mild to severe stages, was conducted. The baseline and follow-up assessments included computed tomography and body plethysmography. Results: This study included 35 patients (17 females and 18 males). This study revealed that females had more stable LF but tended to have greater declines in SMM areas and indices than males (−5.4% vs. −1.9%, respectively), despite the fact that females were younger and had higher LF and less exacerbation than males. A multivariate linear regression showed a negative association between the inspiratory capacity/total lung capacity ratio (IC/TLC) and muscle fat area. Conclusions: The findings suggest distinct LF and BC progression patterns between male and female patients with COPD. A low IC/TLC ratio may predict increased muscle fat. Further studies are necessary to understand these relationships better.
Investigation on UV Degradation and Mechanism of 6:2 Fluorotelomer Sulfonamide Alkyl Betaine, Based on Model Compound Perfluorooctanoic Acid
Authors: Ahmed Naveed , Martienssen Marion , Bryant Isaac Mbir , Vione Davide , Bruzzoniti Maria Concetta , Riedel Ramona
Published: March 2024

The UV treatment of 6:2 FTAB involves the mitigation of this persistent chemical by the impact of ultraviolet radiation, which is known for its resistance to environmental breakdown. UV treatment of PFOA and/or 6:2 FTAB, and the role of responsible species and their mechanism have been presented. Our investigation focused on the degradation of perfluorooctanoic acid (PFOA) and 6:2 fluorotelomer sulfonamide alkyl betaine (6:2 FTAB, Capstone B), using UV photolysis under various pH conditions. Initially, we used PFOA as a reference, finding a 90% decomposition after 360 min at the original (unadjusted) pH 5.6, with a decomposition rate constant of (1.08 ± 0.30) × 10−4 sec−1 and a half-life of 107 ± 2 min. At pH 4 and 7, degradation averaged 85% and 80%, respectively, while at pH 10, it reduced to 57%. For 6:2 FTAB at its natural pH 6.5, almost complete decomposition occurred. The primary UV transformation product was identified as 6:2 fluorotelomer sulfonic acid (6:2 FTSA), occasionally accompanied by shorter-chain perfluoroalkyl acids (PFAAs) including PFHpA, PFHxA, and PFPeA. Interestingly, the overall decomposition percentages were unaffected by pH for 6:2 FTAB, though pH influenced rate constants and half-lives. In PFOA degradation, direct photolysis and reaction with hydrated electrons were presumed mechanisms, excluding the involvement of hydroxyl radicals. The role of superoxide radicals remains uncertain. For 6:2 FTAB, both direct and indirect photolysis were observed, with potential involvement of hydroxyl, superoxide radicals, and/or other reactive oxygen species (ROS). Clarification is needed regarding the role of eaq− in the degradation of 6:2 FTAB.
Can Nano Yield Big Insights? Oligonucleotide-Based Biosensors in Early Diagnosis of Gastric Cancer
Authors: Avanu Alexandra E. , Ciubotariu Alexandra M. , Dodi Gianina
Published: March 2024

Gastric cancer (GC) remains a significant global health challenge, with late-stage diagnosis impacting treatment options and decreased survival rates. To address this, there has been a growing interest in the development of innovative screening and diagnostic methods. Over the past 20 years, nanobiosensors have undergone multiple iterations and unveiled remarkable features that pledge to reshape patient care. Despite the excitement over the plethora of ground-breaking advancements for cancer detection, use-ready samples and streamlined healthcare information monitoring and usage, this technology is still awaiting entry into clinical trials, urging a closer gaze within the medical community. Oligonucleotide-based biosensors, leveraging DNA or RNA’s long-term storage of information, offer great specificity and sensitivity, as described throughout this paper. Consequently, this renders them as an ideal choice for revolutionizing GC diagnosis and facilitating early intervention. The aim of this review is to provide an overview of this cutting-edge, invaluable technology and its limitations across various aspects.