bluuebunny/crossref_metadata_2025 huggingface dataset:
Amphipod diversity and metabolomics of the Antarctic spongeDendrilla antarctica
Authors: von Salm Jacqueline L. , Witowski Christopher G. , Amsler Margaret O. , Amsler Charles D. , McClintock James B. , Baker Bill J.
Published: September 2022

The western Antarctic Peninsula harbours a diverse benthic marine community where dense canopies of macroalgae can dominate the shallow subtidal zone (0–40 m or greater). In the lower portion of this range (below 25–35 m depending on topography), invertebrates such as sponges and echinoderms can be found in greater abundance due to reduced competition for space from the algal species.Dendrilla antarctica(previouslyDendrilla membranosa) is a common demosponge that thrives in both communities and is known for producing diterpene secondary metabolites as a defence against sympatric sea star and amphipod predators. Omnivorous mesograzers such as amphipods inhabit both communities; however, they are in greatest abundance within the macroalgal canopy. Due to the differences between habitats, it was hypothesized that specific amphipod species not susceptible to the defensive metabolites ofD. antarcticawould take refuge from predators in the chemically defended sponge. Analysis of the metabolome and amphipod communities from sponges in both habitats found correlations of metabolic profile to both abundance and habitat. These studies serve to inform our understanding of the complex ecosystem of the Antarctic benthos that stands to be dramatically altered by the rapidly changing climate in the years to come.
Monitoring the Health of Our Planet using Earth Observations
Authors: Kavvada Argyro
Published: September 2022

A new book explores how Earth science knowledge addresses critical global challenges including sustainable development, disaster risk reduction, and climate change.
Separation of Spacecraft Noise From Geomagnetic Field Observations Through Density‐Based Cluster Analysis and Compressive Sensing
Authors: Hoffmann Alex Paul , Moldwin Mark B.
Published: September 2022

The use of magnetometers for space exploration is inhibited by magnetic noise generated by spacecraft electrical systems. Mechanical booms are traditionally used to extend magnetometers away from noise sources. If a spacecraft is equipped with multiple magnetometers, signal processing algorithms can be used to compare magnetometer measurements and remove stray magnetic noise signals. We propose the use of density‐based cluster analysis to identify spacecraft noise signals and compressive sensing to separate spacecraft noise from geomagnetic field data. This method assumes no prior knowledge of the number, location, or amplitude of noise signals, but assumes that they have minimal overlapping spectral properties. We demonstrate the validity of this algorithm by separating high latitude magnetic perturbations recorded by the low‐Earth orbiting satellite, SWARM, from noise signals in simulation and in a laboratory experiment using a mock CubeSat apparatus. In the case of more noise sources than magnetometers, this problem is an instance of underdetermined blind source separation (UBSS). This work presents a UBSS signal processing algorithm to remove spacecraft noise and minimize the need for a mechanical boom.
Rupture Directivity of the 2021MW6.0 Yangbi, Yunnan Earthquake
Authors: Gong Wenzheng , Ye Lingling , Qiu Yuxin , Lay Thorne , Kanamori Hiroo
Published: September 2022

The 2021MW6.0 Yangbi, Yunnan strike‐slip earthquake occurred on an unmapped crustal fault near the Weixi‐Qiaoho‐Weishan Fault along the southeast margin of the Tibetan Plateau. Using near‐source broadband seismic data from ChinArray, we investigate the spatial and temporal rupture evolution of the mainshock using apparent moment‐rate functions (AMRFs) determined by the empirical Green's function (EGF) method. Assuming a 1D line source on the fault plane, the rupture propagated unilaterally southeastward (∼144°) over a rupture length of ∼8.0 km with an estimated rupture speed of 2.1 km/s to 2.4 km/s. A 2D coseismic slip distribution for an assumed maximum rupture propagation speed of 2.2 km/s indicates that the rupture propagated to the southeast ∼8.0 km along strike and ∼5.0 km downdip with a peak slip of ∼2.1 m before stopping near the largest foreshock, where three bifurcating subfaults intersect. Using the AMRFs, the radiated energy of the mainshock is estimated as ∼. The relatively low moment scaled radiated energyof 1.5 × 10−5and intense foreshock and aftershock activity might indicate reactivation of an immature fault. The earthquake sequence is mainly distributed along a northwest‐southeast trend, and aftershocks and foreshocks are distributed near the periphery of the mainshock large‐slip area, suggesting that the stress in the mainshock slip zone is significantly reduced to below the level for more than a few overlapping aftershock to occur.
Limited Earthquake Interaction During a Geothermal Hydraulic Stimulation in Helsinki, Finland
Authors: Kwiatek Grzegorz , Martínez‐Garzón Patricia , Davidsen Jörn , Malin Peter , Karjalainen Aino , Bohnhoff Marco , Dresen Georg
Published: September 2022

We investigate induced seismicity associated with a hydraulic stimulation campaign performed in 2020 in the 5.8 km deep geothermal OTN‐2 well near Helsinki, Finland as part of the St1 Deep Heat project. A total of 2,875 m3 of fresh water was injected during 16 days at well‐head pressures <70 MPa and with flow rates between 400 and 1,000 L/min. The seismicity was monitored using a high‐resolution seismic network composed of 10 borehole geophones surrounding the project site and a borehole array of 10 geophones located in adjacent OTN‐3 well. A total of 6,121 induced earthquakes with local magnitudes were recorded during and after the stimulation campaign. The analyzed statistical parameters include magnitude‐frequency b‐value, interevent time and interevent time ratio, as well as magnitude correlations. We find that the b‐value remained stationary for the entire injection period suggesting limited stress build‐up or limited fracture network coalescence in the reservoir. The seismicity during the stimulation neither shows signatures of magnitude correlations, nor temporal clustering or anticlustering beyond those arising from varying injection rates. The interevent time statistics are characterized by a Poissonian time‐varying distribution. The calculated parameters indicate no earthquake interaction. Focal mechanisms suggest that the injection activated a spatially distributed network of similarly oriented fractures. The seismicity displays stable behavior with no signatures pointing toward a runaway event. The cumulative seismic moment is proportional to the cumulative hydraulic energy and the maximum magnitude is controlled by injection rate. The performed study provides a base for implementation of time‐dependent probabilistic seismic hazard assessment for the project site.
Surface Heating Over the Tibetan Plateau Associated With the Antarctic Oscillation
Authors: Tang Yuheng , Duan Anmin , Hu Jun
Published: September 2022

The surface heating on the Tibetan Plateau (TP) exerts large influence on the Asian summer monsoon. Drivers of TP surface heat include the tropical forcings and other climate modes in middle and high latitude of the Northern Hemisphere, however whether the climate modes in Southern Hemisphere influence the TP surface heat is rarely studied. Using multiple source data diagnosis and numerical experiment from an atmospheric general circulation model (AGCM), we found that the Antarctic Oscillation (AAO) in May can efficiently modulate the subsequent surface heat source over the TP in June. When the AAO is in positive phases, a northeastward propagating atmospheric Rossby wave train originates from the Amundsen Sea low. As a part of this wave train, a pair of anomalous cyclone and anticyclone in the Southern Hemisphere accelerates the surface southeasterlies between them, accompanied by cold sea surface temperature (SST) anomaly in the equatorial middle and eastern Indian Ocean induced by the wind‐evaporation‐SST feedback. Due to the large thermal inertia, in June, the cold SST anomaly stimulates anticyclone anomalies over the western TP and eastern Arabian Sea, which increase the moisture transportation toward the TP and are conducive to the formation and maintenance of the precipitation over the middle and eastern TP. In contrast, the surface heat source, which is dominated by the upward sensible heat flux, is reduced substantially. This result indicates that the AAO can be one of the precursors of the TP heat source, and may help improve the prediction of the Asian summer monsoon.
Longitudinal trends in incidence and health care use for pediatric concussion in Alberta, Canada
Authors: Wittevrongel Krystle , Barrett Olesya , Couloigner Isabelle , Bertazzon Stefania , Hagel Brent , Schneider Kathryn J. , Johnson David , Yeates Keith O. , Zwicker Jennifer D.
Published: September 2022

Background

We described longitudinal trends in the incidence of episodes of care (EOC) and follow-up care for pediatric concussion in relation to age, sex, rurality of patient residence, point of care, and area-based socioeconomic status (SES) in Alberta, Canada.

Methods

A retrospective population-based cohort study was conducted using linked, province-wide administrative health data for all patients <18 years of age who received a diagnosis of concussion, other specified injuries of head, unspecified injury of head, or post-concussion syndrome between April 1, 2004 and March 31, 2018. Data were geospatially mapped.

Results

Concussion EOCs increased 2.2-fold over the study period, follow-up visits 5.1-fold. Care was increasingly received in physician office (PO) settings. Concussion diagnoses in rural and remote areas occurred in emergency department (ED) settings more often than in metro centres or urban areas (76%/75% vs. 52%/60%). Proportion of concussion diagnoses was positively related to SES and age. Diagnosis and point of care varied geographically.

Conclusions

The shift in care to PO settings, increased incidence of all diagnoses, and the higher use of the ED by some segments of the population all have important implications for appropriate clinical management and the efficient provision of health care for pediatric concussion.

Impact

This is the first study to use EOC to describe longitudinal trends in incidence and follow-up care for pediatric concussion in relation to age, sex, rurality, point of care, and area-based SES.

We report increased incidence of concussion in both emergency and outpatient settings and the proportion of diagnoses was positively related to SES and age.

Patients increasingly received care for concussion in PO over time.

Geospatial mapping indicated that the incidence of concussion and unspecified injury of head varied geographically and temporally.

Results have important implications for appropriate clinical management and efficient provision of health care following pediatric concussion.
Plant-associated fungi support bacterial resilience following water limitation
Authors: Hestrin Rachel , Kan Megan , Lafler Marissa , Wollard Jessica , Kimbrel Jeffrey A , Ray Prasun , Blazewicz Steven J , Stuart Rhona , Craven Kelly , Firestone Mary , Nuccio Erin E , Pett-Ridge Jennifer
Published: September 2022

Drought disrupts soil microbial activity and many biogeochemical processes. Although plant-associated fungi can support plant performance and nutrient cycling during drought, their effects on nearby drought-exposed soil microbial communities are not well resolved. We used H218O quantitative stable isotope probing (qSIP) and 16S rRNA gene profiling to investigate bacterial community dynamics following water limitation in the hyphospheres of two distinct fungal lineages (Rhizophagus irregularis and Serendipita bescii) grown with the bioenergy model grass Panicum hallii. In uninoculated soil, a history of water limitation resulted in significantly lower bacterial growth potential and growth efficiency, as well as lower diversity in the actively growing bacterial community. In contrast, both fungal lineages had a protective effect on hyphosphere bacterial communities exposed to water limitation: bacterial growth potential, growth efficiency, and the diversity of the actively growing bacterial community were not suppressed by a history of water limitation in soils inoculated with either fungus. Despite their similar effects at the community level, the two fungal lineages did elicit different taxon-specific responses, and bacterial growth potential was greater in R. irregularis compared to S. bescii-inoculated soils. Several of the bacterial taxa that responded positively to fungal inocula belong to lineages that are considered drought susceptible. Overall, H218O qSIP highlighted treatment effects on bacterial community structure that were less pronounced using traditional 16S rRNA gene profiling. Together, these results indicate that fungal–bacterial synergies may support bacterial resilience to moisture limitation.
Children and adolescents with ASD treated with CBD-rich cannabis exhibit significant improvements particularly in social symptoms: an open label study
Authors: Hacohen Micha , Stolar Orit E. , Berkovitch Matitiahu , Elkana Odelia , Kohn Elkana , Hazan Ariela , Heyman Eli , Sobol Yael , Waissengreen Danel , Gal Eynat , Dinstein Ilan
Published: September 2022

In recent years there has been growing interest in the potential benefits of CBD-rich cannabis treatment for children with ASD. Several open label studies and one double-blind placebo-controlled study have reported that CBD-rich cannabis is safe and potentially effective in reducing disruptive behaviors and improving social communication. However, previous studies have mostly based their conclusions on parental reports without the use of standardized clinical assessments. Here, we conducted an open label study to examine the efficacy of 6 months of CBD-rich cannabis treatment in children and adolescents with ASD. Longitudinal changes in social communication abilities and restricted and repetitive behaviors (RRB) were quantified using parent report with the Social Responsiveness Scale and clinical assessment with the Autism Diagnostic Observation Schedule (ADOS). We also quantified changes in adaptive behaviors using the Vineland, and cognitive abilities using an age-appropriate Wechsler test. Eighty-two of the 110 recruited participants completed the 6-month treatment protocol. While some participants did not exhibit any improvement in symptoms, there were overall significant improvements in social communication abilities as quantified by the ADOS, SRS, and Vineland with larger improvements in participants who had more severe initial symptoms. Significant improvements in RRB were noted only with parent-reported SRS scores and there were no significant changes in cognitive scores. These findings suggest that treatment with CBD-rich cannabis can yield improvements, particularly in social communication abilities, which were visible even when using standardized clinical assessments. Additional double-blind placebo-controlled studies utilizing standardized assessments are highly warranted for substantiating these findings.
Everlasting impact of initial perturbations on first-passage times of non-Markovian random walks
Authors: Levernier N. , Mendes T. V. , Bénichou O. , Voituriez R. , Guérin T.
Published: September 2022

Persistence, defined as the probability that a signal has not reached a threshold up to a given observation time, plays a crucial role in the theory of random processes. Often, persistence decays algebraically with time with non trivial exponents. However, general analytical methods to calculate persistence exponents cannot be applied to the ubiquitous case of non-Markovian systems relaxing transiently after an imposed initial perturbation. Here, we introduce a theoretical framework that enables the non-perturbative determination of persistence exponents of Gaussian non-Markovian processes with non stationary dynamics relaxing to a steady state after an initial perturbation. Two situations are analyzed: either the system is subjected to a temperature quench at initial time, or its past trajectory is assumed to have been observed and thus known. Our theory covers the case of spatial dimension higher than one, opening the way to characterize non-trivial reaction kinetics for complex systems with non-equilibrium initial conditions.
Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria
Authors: Weng Yuzhe , Shepherd Dawn , Liu Yi , Krishnan Nitya , Robertson Brian D. , Platt Nick , Larrouy-Maumus Gerald , Platt Frances M.
Published: September 2022

Mycobacterium tuberculosis(Mtb) survives and replicates within host macrophages (MΦ) and subverts multiple antimicrobial defense mechanisms. Previously, we reported that lipids shed by pathogenic mycobacteria inhibit NPC1, the lysosomal membrane protein deficient in the lysosomal storage disorder Niemann-Pick disease type C (NPC). Inhibition of NPC1 leads to a drop in lysosomal calcium levels, blocking phagosome-lysosome fusion leading to mycobacterial survival. We speculated that the production of specific cell wall lipid(s) that inhibit NPC1 could have been a critical step in the evolution of pathogenicity. We therefore investigated whether lipid extracts from clinicalMtbstrains from multipleMtblineages,Mtbcomplex (MTBC) members and non-tubercular mycobacteria (NTM) inhibit the NPC pathway. We report that inhibition of the NPC pathway was present in all clinical isolates fromMtblineages 1, 2, 3 and 4,Mycobacterium bovisand the NTM,Mycobacterium abscessusandMycobacterium avium. However, lipid extract fromMycobacterium canettii, which is considered to resemble the common ancestor of the MTBC did not inhibit the NPC1 pathway. We conclude that the evolution of NPC1 inhibitory mycobacterial cell wall lipids evolved early and post divergence fromMycobacterium canettii-related mycobacteria and that this activity contributes significantly to the promotion of disease.
In vivo tumor immune microenvironment phenotypes correlate with inflammation and vasculature to predict immunotherapy response
Authors: Sahu Aditi , Kose Kivanc , Kraehenbuehl Lukas , Byers Candice , Holland Aliya , Tembo Teguru , Santella Anthony , Alfonso Anabel , Li Madison , Cordova Miguel , Gill Melissa , Fox Christi , Gonzalez Salvador , Kumar Piyush , Wang Amber Weiching , Kurtansky Nicholas , Chandrani Pratik , Yin Shen , Mehta Paras , Navarrete-Dechent Cristian , Peterson Gary , King Kimeil , Dusza Stephen , Yang Ning , Liu Shuaitong , Phillips William , Guitera Pascale , Rossi Anthony , Halpern Allan , Deng Liang , Pulitzer Melissa , Marghoob Ashfaq , Chen Chih-Shan Jason , Merghoub Taha , Rajadhyaksha Milind
Published: September 2022

Response to immunotherapies can be variable and unpredictable. Pathology-based phenotyping of tumors into ‘hot’ and ‘cold’ is static, relying solely on T-cell infiltration in single-time single-site biopsies, resulting in suboptimal treatment response prediction. Dynamic vascular events (tumor angiogenesis, leukocyte trafficking) within tumor immune microenvironment (TiME) also influence anti-tumor immunity and treatment response. Here, we report dynamic cellular-level TiME phenotyping in vivo that combines inflammation profiles with vascular features through non-invasive reflectance confocal microscopic imaging. In skin cancer patients, we demonstrate three main TiME phenotypes that correlate with gene and protein expression, and response to toll-like receptor agonist immune-therapy. Notably, phenotypes with high inflammation associate with immunostimulatory signatures and those with high vasculature with angiogenic and endothelial anergy signatures. Moreover, phenotypes with high inflammation and low vasculature demonstrate the best treatment response. This non-invasive in vivo phenotyping approach integrating dynamic vasculature with inflammation serves as a reliable predictor of response to topical immune-therapy in patients.
GLH/VASA helicases promote germ granule formation to ensure the fidelity of piRNA-mediated transcriptome surveillance
Authors: Chen Wenjun , Brown Jordan S. , He Tao , Wu Wei-Sheng , Tu Shikui , Weng Zhiping , Zhang Donglei , Lee Heng-Chi
Published: September 2022

piRNAs function as guardians of the genome by silencing non-self nucleic acids and transposable elements in animals. Many piRNA factors are enriched in perinuclear germ granules, but whether their localization is required for piRNA biogenesis or function is not known. Here we show that GLH/VASA helicase mutants exhibit defects in forming perinuclear condensates containing PIWI and other small RNA cofactors. These mutant animals produce largely normal levels of piRNA but are defective in triggering piRNA silencing. Strikingly, while many piRNA targets are activated in GLH mutants, we observe that hundreds of endogenous genes are aberrantly silenced by piRNAs. This defect in self versus non-self recognition is also observed in other mutants where perinuclear germ granules are disrupted. Together, our results argue that perinuclear germ granules function critically to promote the fidelity of piRNA-based transcriptome surveillance in C. elegans and preserve self versus non-self distinction.
High performance polymerized small molecule acceptor by synergistic optimization on π-bridge linker and side chain
Authors: Sun Guangpei , Jiang Xin , Li Xiaojun , Meng Lei , Zhang Jinyuan , Qin Shucheng , Kong Xiaolei , Li Jing , Xin Jingming , Ma Wei , Li Yongfang
Published: September 2022

The polymerized small-molecule acceptors have attracted great attention for application as polymer acceptor in all-polymer solar cells recently. The modification of small molecule acceptor building block and the π-bridge linker is an effective strategy to improve the photovoltaic performance of the polymer acceptors. In this work, we synthesized a new polymer acceptor PG-IT2F which is a modification of the representative polymer acceptor PY-IT by replacing its upper linear alkyl side chains on the small molecule building block with branched alkyl chains and attaching difluorene substituents on its thiophene π-bridge linker. Through this synergistic optimization, PG-IT2F possesses more suitable phase separation, increased charge transportation, better exciton dissociation, lower bimolecular recombination, and longer charge transfer state lifetime than PY-IT in their polymer solar cells with PM6 as polymer donor. Therefore, the devices based on PM6:PG-IT2F demonstrated a high power conversion efficiency of 17.24%, which is one of the highest efficiency reported for the binary all polymer solar cells to date. This work indicates that the synergistic regulation of small molecule acceptor building block and π-bridge linker plays a key role in designing and developing highly efficient polymer acceptors.
Integrating and formatting biomedical data as pre-calculated knowledge graph embeddings in the Bioteque
Authors: Fernández-Torras Adrià , Duran-Frigola Miquel , Bertoni Martino , Locatelli Martina , Aloy Patrick
Published: September 2022

Biomedical data is accumulating at a fast pace and integrating it into a unified framework is a major challenge, so that multiple views of a given biological event can be considered simultaneously. Here we present the Bioteque, a resource of unprecedented size and scope that contains pre-calculated biomedical descriptors derived from a gigantic knowledge graph, displaying more than 450 thousand biological entities and 30 million relationships between them. The Bioteque integrates, harmonizes, and formats data collected from over 150 data sources, including 12 biological entities (e.g., genes, diseases, drugs) linked by 67 types of associations (e.g., ‘drug treats disease’, ‘gene interacts with gene’). We show how Bioteque descriptors facilitate the assessment of high-throughput protein-protein interactome data, the prediction of drug response and new repurposing opportunities, and demonstrate that they can be used off-the-shelf in downstream machine learning tasks without loss of performance with respect to using original data. The Bioteque thus offers a thoroughly processed, tractable, and highly optimized assembly of the biomedical knowledge available in the public domain.
Mitigation of China’s carbon neutrality to global warming
Authors: Li Longhui , Zhang Yue , Zhou Tianjun , Wang Kaicun , Wang Can , Wang Tao , Yuan Linwang , An Kangxin , Zhou Chenghu , Lü Guonian
Published: September 2022

Projecting mitigations of carbon neutrality from individual countries in relation to future global warming is of great importance for depicting national climate responsibility but is poorly quantified. Here, we show that China’s carbon neutrality (CNCN) can individually mitigate global warming by 0.48 °C and 0.40 °C, which account for 14% and 9% of the global warming over the long term under the shared socioeconomic pathway (SSP) 3-7.0 and 5-8.5 scenarios, respectively. Further incorporating changes in CH4 and N2O emissions in association with CNCN together will alleviate global warming by 0.21 °C and 0.32 °C for SSP1-2.6 and SSP2-4.5 over the long term, and even by 0.18 °C for SSP2-4.5 over the mid-term, but no significant impacts are shown for all SSPs in the near term. Divergent responses in alleviated warming are seen at regional scales. The results provide a useful reference for the global stocktake, which assesses the collective progress towards the climate goals of the Paris Agreement.
Atherogenic potential of microgravity hemodynamics in the carotid bifurcation: a numerical investigation
Authors: Sucosky Philippe , Kalaiarasan Varun Vinayak , Quasebarth Graham B. , Strack Patricia , Shar Jason A.
Published: September 2022

Long-duration spaceflight poses multiple hazards to human health, including physiological changes associated with microgravity. The hemodynamic adaptations occurring upon entry into weightlessness have been associated with retrograde stagnant flow conditions and thromboembolic events in the venous vasculature but the impact of microgravity on cerebral arterial hemodynamics and function remains poorly understood. The objective of this study was to quantify the effects of microgravity on hemodynamics and wall shear stress (WSS) characteristics in 16 carotid bifurcation geometries reconstructed from ultrasonography images using computational fluid dynamics modeling. Microgravity resulted in a significant 21% increase in flow stasis index, a 22–23% decrease in WSS magnitude and a 16–26% increase in relative residence time in all bifurcation branches, while preserving WSS unidirectionality. In two anatomies, however, microgravity not only promoted flow stasis but also subjected the convex region of the external carotid arterial wall to a moderate increase in WSS bidirectionality, which contrasted with the population average trend. This study suggests that long-term exposure to microgravity has the potential to subject the vasculature to atheroprone hemodynamics and this effect is modulated by subject-specific anatomical features. The exploration of the biological impact of those microgravity-induced WSS aberrations is needed to better define the risk posed by long spaceflights on cardiovascular health.
Corrosion behavior of TiZrHfBeCu(Ni) high-entropy bulk metallic glasses in 3.5 wt. % NaCl
Authors: Gong Pan , Wang Dongliang , Zhang Cheng , Wang Ying , Jamili-Shirvan Zahra , Yao Kefu , Wang Xinyun
Published: September 2022

The corrosion behavior of TiZrHfBeCu(Ni) high-entropy bulk metallic glasses (HE-BMGs) has been investigated. The TiZrHfBeCu(Ni) HE-BMGs exhibited high corrosion resistance in 3.5 wt. % NaCl solution because of accumulation of ZrO2 and TiO2 in the passive film. Ni promoted increases of the ZrO2, TiO2, and HfO2 contents and a decrease of the BeO content, which improved the HE-BMG corrosion behavior. Compared with Zr41.2Ti13.8Ni10Cu12.5Be22.5 BMG, the high-entropy effect of HE-BMGs can significantly reduce the atomic mobility, which inhibits outward migration of Cu, reduces the kinetics of the dissolution reaction, and inhibits inward erosion by Cl−, thereby improving the corrosion performance.
Nanoscale electric field imaging with an ambient scanning quantum sensor microscope
Authors: Qiu Ziwei , Hamo Assaf , Vool Uri , Zhou Tony X. , Yacoby Amir
Published: September 2022

Nitrogen-vacancy (NV) center in diamond is a promising quantum sensor with remarkably versatile sensing capabilities. While scanning NV magnetometry is well-established, NV electrometry has been so far limited to bulk diamonds. Here we demonstrate imaging external alternating (AC) and direct (DC) electric fields with a single NV at the apex of a diamond scanning tip under ambient conditions. A strong electric field screening effect is observed at low frequencies. We quantitatively measure its frequency dependence and overcome this screening by mechanically oscillating the tip for imaging DC fields. Our scanning NV electrometry achieved an AC E-field sensitivity of 26 mV μm−1 Hz−1/2, a DC E-field gradient sensitivity of 2 V μm−2 Hz−1/2, and sub-100 nm resolution limited by the NV-sample distance. Our work represents an important step toward building a scanning-probe-based multimodal quantum sensing platform.
Socioeconomic and genomic roots of verbal ability from current evidence
Authors: Guo Guang , Lin Meng-Jung , Harris Kathleen Mullan
Published: September 2022

This research examines how the human genome and SES jointly and interactively shape verbal ability among youth in the U.S. The youth are aged 12–18 when the study starts. The research draws on findings from the latest GWAS as well as a rich set of longitudinal SES measures at individual, family and neighborhood levels from Add Health (N = 7194). Both SES and genome measures predict verbal ability well separately and jointly. More interestingly, the inclusion of both sets of predictors in the same model corrects for about 20% upward bias in the effect of the education PGS, and implies that about 20–30% of the effects of parental SES are not environmental, but parentally genomic. The three incrementalR2s that measure the relative contributions of the two PGSs, the genomic component in parental SES, and the environmental component in parental SES are estimated to be about 1.5%, 1.5%, and 7.8%, respectively. The total environmentalR2and the total genomicR2are, thus, 7.8% and 3%, respectively. These findings confirm the importance of SES environment and also pose challenges to traditional social-science research. Not only does an individual’s genome have an important direct influence on verbal ability, parental genomes also influence verbal ability through parental SES. The decades-long blueprint of including SES in a model and interpreting their effects as those of SES needs to be amended accordingly. A straightforward solution is to routinely collect DNA data for large social-science studies granted that the primary purpose is to understand social and environmental influences.
The personality and cognitive traits associated with adolescents’ sensitivity to social norms
Authors: Tate Christopher , Kumar Rajnish , Murray Jennifer M. , Sanchez-Franco Sharon , Sarmiento Olga L. , Montgomery Shannon C. , Zhou Huiyu , Ramalingam Abhijit , Krupka Erin , Kimbrough Erik , Kee Frank , Hunter Ruth F.
Published: September 2022

Little is known about the personality and cognitive traits that shape adolescents’ sensitivity to social norms. Further, few studies have harnessed novel empirical tools to elicit sensitivity to social norms among adolescent populations. This paper examines the association between sensitivity to norms and various personality and cognitive traits using an incentivised rule-following task grounded in Game Theory. Cross-sectional data were obtained from 1274 adolescents. Self-administered questionnaires were used to measure personality traits as well as other psychosocial characteristics. Incentivised rule-following experiments gauged sensitivity to social norms. A series of multilevel mixed effects ordered logistic regression models were employed to assess the association between sensitivity to norms and the personality and cognitive traits. The results highlighted statistically significant univariate associations between the personality and cognitive traits and sensitivity to norms. However, in the multivariate adjusted model, the only factor associated with sensitivity to norms was gender. The gender-stratified analyses revealed differences in the personality and cognitive traits associated with sensitivity to norms across genders. For males need to belong was significantly negatively associated with sensitivity to norms in the multivariate model. By comparison, emotional stability was negatively associated with sensitivity to norms for females. This study reinforced the findings from an earlier study and suggested female adolescents had higher levels of sensitivity to norms. The results indicated no consistent pattern between sensitivity to norms and the personality and cognitive traits. Our findings provide a basis for further empirical research on a relatively nascent construct, and bring a fresh perspective to the question of norm-following preferences among this age group.
Deep learning multi-organ segmentation for whole mouse cryo-images including a comparison of 2D and 3D deep networks
Authors: Liu Yiqiao , Gargesha Madhusudhana , Scott Bryan , Tchilibou Wane Arthure Olivia , Wilson David L.
Published: September 2022

Cryo-imaging provided 3D whole-mouse microscopic color anatomy and fluorescence images that enables biotechnology applications (e.g., stem cells and metastatic cancer). In this report, we compared three methods of organ segmentation: 2D U-Net with 2D-slices and 3D U-Net with either 3D-whole-mouse or 3D-patches. We evaluated the brain, thymus, lung, heart, liver, stomach, spleen, left and right kidney, and bladder. Training with 63 mice, 2D-slices had the best performance, with median Dice scores of > 0.9 and median Hausdorff distances of < 1.2 mm in eightfold cross validation for all organs, except bladder, which is a problem organ due to variable filling and poor contrast. Results were comparable to those for a second analyst on the same data. Regression analyses were performed to fit learning curves, which showed that 2D-slices can succeed with fewer samples. Review and editing of 2D-slices segmentation results reduced human operator time from ~ 2-h to ~ 25-min, with reduced inter-observer variability. As demonstrations, we used organ segmentation to evaluate size changes in liver disease and to quantify the distribution of therapeutic mesenchymal stem cells in organs. With a 48-GB GPU, we determined that extra GPU RAM improved the performance of 3D deep learning because we could train at a higher resolution.
Association of Toll-like receptors polymorphisms with the risk of acute lymphoblastic leukemia in the Brazilian Amazon
Authors: Xabregas Lilyane Amorim , Hanna Fabíola Silva Alves , Magalhães-Gama Fábio , Souza Gláucia Lima , Pereira Daniele Sá , de Lima Amanda Barros , Toro Diana Mota , Santiago Mirian Rodrigues Ribeiro , da Motta Passos Leny Nascimento , Tarragô Andréa Monteiro , Malheiro Adriana , Costa Allyson Guimarães
Published: September 2022

Acute lymphoblastic leukemia (ALL) is the most common hematologic malignancy in children in childhood. Single-nucleotide polymorphism (SNPs) in key molecules of the immune system, such as Toll-like receptors (TLRs) and CD14 molecules, are associated with the development of several diseases. However, their role in ALL is unknown. A case–control study was performed with 152 ALL patients and 187 healthy individuals to investigate the role of SNPs in TLRs and the CD14 gene in ALL. In this study, TLR6 C > T rs5743810 [OR: 3.20, 95% CI: 1.11–9.17, p = 0.003) and TLR9 C > T rs187084 (OR: 2.29, 95% CI: 1.23–4.26, p = 0.000) seems to be a risk for development of ALL. In addition, the TLR1 T > G rs5743618 and TLR6 C > T rs5743810 polymorphisms with protection against death (OR: 0.17, 95% IC: 0.04–0.79, p = 0.008; OR: 0.48, 95% IC: 0.24–0.94, p = 0.031, respectively). Our results show that SNPs in TLRs genes may be involved in the pathogenesis of ALL and may influence clinical prognosis; however, further studies are necessary to elucidate the role of TLR1, TLR4, TLR5, TLR6, TLR9 and CD14 polymorphisms in this disease.
Athleticism and sex impact neural processing of sound
Authors: Krizman Jennifer , Bonacina Silvia , Colegrove Danielle , Otto-Meyer Rembrandt , Nicol Trent , Kraus Nina
Published: September 2022

Biology and experience both influence the auditory brain. Sex is one biological factor with pervasive effects on auditory processing. Females process sounds faster and more robustly than males. These differences are linked to hormone differences between the sexes. Athleticism is an experiential factor known to reduce ongoing neural noise, but whether it influences how sounds are processed by the brain is unknown. Furthermore, it is unknown whether sports participation influences auditory processing differently in males and females, given the well-documented sex differences in auditory processing seen in the general population. We hypothesized that athleticism enhances auditory processing and that these enhancements are greater in females. To test these hypotheses, we measured auditory processing in collegiate Division I male and female student-athletes and their non-athlete peers (total n = 1012) using the frequency-following response (FFR). The FFR is a neurophysiological response to sound that reflects the processing of discrete sound features. We measured across-trial consistency of the response in addition to fundamental frequency (F0) and harmonic encoding. We found that athletes had enhanced encoding of the harmonics, which was greatest in the female athletes, and that athletes had more consistent responses than non-athletes. In contrast, F0 encoding was reduced in athletes. The harmonic-encoding advantage in female athletes aligns with previous work linking harmonic encoding strength to female hormone levels and studies showing estrogen as mediating athlete sex differences in other sensory domains. Lastly, persistent deficits in auditory processing from previous concussive and repetitive subconcussive head trauma may underlie the reduced F0 encoding in athletes, as poor F0 encoding is a hallmark of concussion injury.
Chromosomal microarray analyses from 5778 patients with neurodevelopmental disorders and congenital anomalies in Brazil
Authors: Krepischi Ana C. V. , Villela Darine , da Costa Silvia Souza , Mazzonetto Patricia C. , Schauren Juliana , Migliavacca Michele P. , Milanezi Fernanda , Santos Juliana G. , Guida Gustavo , Guarischi-Sousa Rodrigo , Campana Gustavo , Kok Fernando , Schlesinger David , Kitajima Joao Paulo , Campagnari Francine , Bertola Debora R. , Vianna-Morgante Angela M. , Pearson Peter L. , Rosenberg Carla
Published: September 2022

Chromosomal microarray analysis (CMA) has been recommended and practiced routinely since 2010 both in the USA and Europe as the first-tier cytogenetic test for patients with unexplained neurodevelopmental delay/intellectual disability, autism spectrum disorders, and/or multiple congenital anomalies. However, in Brazil, the use of CMA is still limited, due to its high cost and complexity in integrating the results from both the private and public health systems. Although Brazil has one of the world’s largest single-payer public healthcare systems, nearly all patients referred for CMA come from the private sector, resulting in only a small number of CMA studies in Brazilian cohorts. To date, this study is by far the largest Brazilian cohort (n = 5788) studied by CMA and is derived from a joint collaboration formed by the University of São Paulo and three private genetic diagnostic centers to investigate the genetic bases of neurodevelopmental disorders and congenital abnormalities. We identified 2,279 clinically relevant CNVs in 1886 patients, not including the 26 cases of UPD found. Among detected CNVs, the corresponding frequency of each category was 55.6% Pathogenic, 4.4% Likely Pathogenic and 40% VUS. The diagnostic yield, by taking into account Pathogenic, Likely Pathogenic and UPDs, was 19.7%. Since the rational for the classification is mostly based on Mendelian or highly penetrant variants, it was not surprising that a second event was detected in 26% of those cases of predisposition syndromes. Although it is common practice to investigate the inheritance of VUS in most laboratories around the world to determine the inheritance of the variant, our results indicate an extremely low cost–benefit of this approach, and strongly suggest that in cases of a limited budget, investigation of the parents of VUS carriers using CMA should not be prioritized.
Numerical investigation on functional limitations of the anti-stall fin for an axial fan: one-factor analyses
Authors: Kim Yong-In , Yang Hyeon-Mo , Lee Kyoung-Yong , Choi Young-Seok
Published: September 2022

The stall in an axial fan is directly related to detrimental phenomena such as performance degradation, vibration, noise, and flow instability at low flow rates. As a kind of passive control method to handle the stall, two-dimensional plates so-named anti-stall fin (ASF) were suggested by ourselves and were attached inside the casing. In this study, the ASF's effect on the internal flow pattern was visually investigated in the flow passage, and its tendency was discussed with the performance curve. Subsequently, the ASF's functional limitations for various design parameters, which the ASF can derive aerodynamically, were presented as the primary focus of this study. Each one-factor analysis was performed, and the internal flow pattern was observed in parallel at the point where the ASF lost its function. For the radial length, axial length, number of fins, and positive-tangential angle, the ASF almost retained its function up to the limitation to prevent instability but radically lost its function at a certain flow rate. For the axial gap and negative-tangential angle, the ASF gradually lost its function. Mostly, this study was based on numerical analysis, and the performance was validated through experimental tests.
Chronic back pain sub-grouped via psychosocial, brain and physical factors using machine learning
Authors: Tagliaferri Scott D. , Wilkin Tim , Angelova Maia , Fitzgibbon Bernadette M. , Owen Patrick J. , Miller Clint T. , Belavy Daniel L.
Published: September 2022

Chronic back pain (CBP) is heterogenous and identifying sub-groups could improve clinical decision making. Machine learning can build upon prior sub-grouping approaches by using a data-driven approach to overcome clinician subjectivity, however, only binary classification of pain versus no-pain has been attempted to date. In our cross-sectional study, age- and sex-matched participants with CBP (n = 4156) and pain-free controls (n = 14,927) from the UkBioBank were included. We included variables of body mass index, depression, loneliness/social isolation, grip strength, brain grey matter volumes and functional connectivity. We used fuzzy c-means clustering to derive CBP sub-groups and Support Vector Machine (SVM), Naïve Bayes, k-Nearest Neighbour (kNN) and Random Forest classifiers to determine classification accuracy. We showed that two variables (loneliness/social isolation and depression) and five clusters were optimal for creating sub-groups of CBP individuals. Classification accuracy was greater than 95% for when CBP sub-groups were assessed only, while misclassification in CBP sub-groups increased to 35–53% across classifiers when pain-free controls were added. We showed that individuals with CBP could sub-grouped and accurately classified. Future research should optimise variables by including specific spinal, psychosocial and nervous system measures associated with CBP to create more robust sub-groups that are discernible from pain-free controls.
Untargeted ultra-high-resolution mass spectrometry metabolomic profiling of blood serum in bladder cancer
Authors: Nizioł Joanna , Ossoliński Krzysztof , Płaza-Altamer Aneta , Kołodziej Artur , Ossolińska Anna , Ossoliński Tadeusz , Ruman Tomasz
Published: September 2022

Bladder cancer (BC) is a common urological cancer of high mortality and recurrence rates. Currently, cystoscopy is performed as standard examination for the diagnosis and subsequent monitoring for recurrence of the patients. Frequent expensive and invasive procedures may deterrent patients from regular follow-up screening, therefore it is important to look for new non-invasive methods to aid in the detection of recurrent and/or primary BC. In this study, ultra-high-performance liquid chromatography coupled with ultra-high-resolution mass spectrometry was employed for non-targeted metabolomic profiling of 200 human serum samples to identify biochemical signatures that differentiate BC from non-cancer controls (NCs). Univariate and multivariate statistical analyses with external validation revealed twenty-seven metabolites that differentiate between BC patients from NCs. Abundances of these metabolites displayed statistically significant differences in two independent training and validation sets. Twenty-three serum metabolites were also found to be distinguishing between low- and high-grade of BC patients and controls. Thirty-seven serum metabolites were found to differentiate between different stages of BC. The results suggest that measurement of serum metabolites may provide more facile and less invasive diagnostic methodology for detection of bladder cancer and recurrent disease management.
Simultaneous speciation of chromate, molybdate and arsenate in lysimetric water from geotechnical composites installed in field lysimeters
Authors: Djurić Marija , Levstek Lucija , Oprčkal Primož , Mladenovič Ana , Pranjić Alenka Mauko , Ščančar Janez , Milačič Radmila
Published: September 2022

Anion-exchange high performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was used for simultaneous speciation of chromate, molybdate and arsenate. The repeatability of measurement tested for multielemental standard solution of chromate, molybdate and arsenate (50 ng mL−1 of Cr, Mo and As, pH 12) was ± 0.9%, ± 4.9% and ± 4.1%, respectively. Limits of quantification (LOQs) were low (0.53 ng mL−1 for chromate and arsenate and 1.03 ng mL−1 for molybdate, expressed as elemental concentrations). A wide linear concentration range (from LOQs to 500 ng mL−1) was obtained. The performances of this method enabled simultaneous speciation analysis in samples of water from lysimeters, in which three geotechnical composites, made of recycled waste, were installed in parallel in compacted and uncompacted, 20 times less dense form. The release of toxic chemical species of elements into lysimetric waters from each composite was studied. The results revealed that the degree of compaction and the composition of composites both have a significant influence on leaching of chromate, molybdate and arsenate. The study proved that multielemental speciation analysis is fast and cost-effective method for investigations of environmental impacts of materials, made from recycled waste, and can be used in other similar applications.