bluuebunny/crossref_metadata_2025 huggingface dataset:
MENYOAL KEMBALI PERKAWINAN DI BAWAH TANGAN (NIKAH SIRRI) DI INDONESIA
Authors: Abdullah Nafilah
Published: January 2018

All people who enter the gates of family life through marriages intend to achieve a  prosperous and harmonious family life and receive blessing in this world and the next. Marriages must therefore be done in accordance with religious law. A marriage is legal if conducted in accordance with one’s religion, and must be recorded in the Office of Religious Affairs (KUA). Marriages which are not recorded by authorized officials are sirri marriages, and are contrary to legislation. Furthermore, its harm can be seen from its consequence, the lack of legal certainty between husband and wife and the children. This entails the legal consequence that sirri marriages do not have a Marital Deed and children borne of such marriage receive no government recognition, and are considered children born out of wedlock, only having civil relations with the mother and the mother’s family. The wife and children abandoned by a biological father cannot take legal action for the fulfillment of their economic rights or for joint marital assets.
MENGURAI BENANG KUSUT DUALISME NIKAH SIRRI (Upaya Meratifikasi Rancangan Undang-Undang Nikah Sirri)
Authors: Suhaeri Suhaeri
Published: January 2018

The emergence of the Draft Bill on the Religious Judiciary Material Law on  Marriage -otherwise known with the Draft Bill on Sirri Marriage- has brought a lengthy polemic debate. This issue essentially rests on a legal matter, the duality of Sirri marriages; which are legal according to Islamic law but illegal according to formal law (national civil law). This led many actors to disregard the need for the ratification of the Draft Bill, where in fact the Draft Bill’s ratification intends to uphold justice. This shows that there is a faulty perception by society in understanding Islamic law’s position on Sirri marriages. Therefore, this article will attempt to rectify society’s understanding on Sirri marriage through the fiqh approach. The conclusion which may be drawn are that Sirri marriages contain two harms (kemafsadatan) at the same time (adultery and harming others). According to fiqh, when a choice exists between two harms, the avoidance of the greater should be chosen. In this case, the greater harm comes from harming wives and children in Sirri marriages. Therefore, good muslims should not conduct Sirri marriages. The reality whereby many Sirri marriages are still conducted shows the need for legal enforcement through the ratification of the Draft Bill.
Existence and Multiplicity of Solutions for Resonant (p,2)-Equations
Authors: Papageorgiou Nikolaos S. , Rădulescu Vicenţiu D. , Repovš Dušan D.
Published: January 2018

We consider Dirichlet elliptic equations driven by the sum of a p-Laplacian

(

2
<
p

)

{(2<p)}

and a Laplacian. The conditions on the reaction term imply that the problem is resonant at both

±
∞

{\pm\infty}

and at zero. We prove an existence theorem (producing one nontrivial smooth solution) and a multiplicity theorem (producing five nontrivial smooth solutions, four of constant sign and the fifth nodal; the solutions are ordered). Our approach uses variational methods and critical groups.
On the Performance and Energy Consumption of Molecular Dynamics Applications for Heterogeneous CPU-GPU Platforms Based on Gromacs
Authors: Poghosyan Armen , Astsatryan Hrachya , Narsisian Wahi , Mamasakhlisov Yevgeni
Published: January 2018

High Performance Computing (HPC) accelerates life science discoveries by enabling scientists to analyze large data sets, to develop detailed models of entire biological systems and to simulate complex biological processes. As computational experiments, molecular dynamics simulations are widely used in life sciences to evaluate the equilibrium nature of classical many-body systems The modelling and molecular dynamics study of surfactant, polymer solutions and the stability of proteins and nucleic acids under different conditions, as well as deoxyribonucleic acid proteins are studied. The study aims to understand the scaling behavior of Gromacs (Groningen machine for chemical simulations) on various platforms, and the maximum performance in the prospect of energy consumption that can be accomplished by tuning the hardware and software parameters. Different system sizes (48K, 64K, and 272K) from scientific investigations have been studied show that the GPU (Graphics Processing Unit) scales rather beneficial than other resources, i.e., with GPU support. We track 2-3 times speedup compared to the latest multi-core CPUs. However, the so-called “threading effect” leads to the better results.
Increased serum concentrations of soluble ST2 are associated with pulmonary complications and mortality in polytraumatized patients
Authors: Haider Thomas , Simader Elisabeth , Hacker Philipp , Ankersmit Hendrik J. , Heinz Thomas , Hajdu Stefan , Negrin Lukas L.
Published: January 2018

Background:

We sought to evaluate the role of soluble ST2 (suppression of tumorigenicity) serum concentrations in polytraumatized patients and its potential role as biomarker for pulmonary complications.

Methods:

We included severely injured patients (injury severity score≥16) admitted to our level I trauma center and analyzed serum samples obtained on the day of admission and on day 2. Furthermore, patients with isolated thoracic injury and healthy probands were included and served as control groups. Serum samples were analyzed for soluble ST2 concentrations with a commercially available ELISA kit.

Results:

A total of 130 patients were included in the present study. Five patients with isolated thoracic injury and eight healthy probands were further included. Serum analyses revealed significantly elevated concentrations of soluble ST2 in polytraumatized patients compared to patients suffering from isolated thoracic trauma and healthy probands. In polytraumatized patients who developed pulmonary complications (acute respiratory distress syndrome and pneumonia) and in patients who died, significantly higher serum concentrations of soluble ST2 were found on day 2 (p<0.001). Serum concentrations of soluble ST2 on day 2 were of prognostic value to predict pulmonary complications in polytraumatized patients (area under the curve=0.720, 95% confidence interval=0.623–0.816). Concomitant thoracic trauma had no further impact on serum concentrations of soluble ST2.

Conclusions:

Serum concentrations of soluble ST2 are upregulated following polytrauma. Increased concentrations were associated with worse outcome.
Assessment of MT-279 compound effect on physical endurance of rats under conditions of chronic hypokinesia
Authors: Saienko Andrii , Voloshchuk Natalia , Toziuk Olena , Kryvoviaz Olena , Kryvoviaz Serhii , Koval Vasyl
Published: January 2018

The objective of the study was to assess the effect of a sodium salt 3-(2-oxo-3-phenyl- 2H-[1,2,4]triazine[2,3-c]quinazolin-6-yl) of propanoic acid (MT-279 compound) on physical endurance of rats under conditions of chronic hypokinesia (15 days) in comparison with 2-ethylthiobenzimidazole hydrobromide (2-ETBI). The results of the conducted experiment showed that chronic hypokinesia (15 days) caused a progressive decrease in the physical endurance of animals. The signs of this phenomenon occurred already on the Day 7 of the experiment. It manifested by a significant decrease in the duration of rats swimming, treadmill runtime and holding on rotating rods (rotarod test). The course of MT-279 compound (2.5 mg/kg, ip) administered to animals in the specified experimental conditions contributed to restoration of physical performance of the study subjects. Upon introduction of the compound, the duration of rats` swimming on Day 7 of the experiment increased by 108.2%, and on Day 15 - by 64.2%. Administration of 2-ETBI caused the growth of this indicator by 70.5% and 24.2%, respectively. Along with this, we revealed an increase of rats` treadmill running time. On Day 7 and Day 15 of the experiment, we recorded 55.2% and 68.4% growth of the indicator, respectively. Daily administration of 2-ETBI contributed to 41.7% and 59.7% increase of running time, respectively. On Day 7 of MT-279 compound administration under the conditions of chronic hypokinesia, the duration of rats` rod retention time increased by 58.7% compared to 6.2% for 2-ETBI. On Day 15 of the experiment, the physical endurance of animals assessed under this test compared to the control group was 89.0% and 72.3%, respectively. On Day 7, MT-279 compound was significantly superior to the comparator drug in all tests performed for assessment of the ability to improve physical endurance under conditions of chronic hypokinesia. On Day 15 of study, the statistically significant superiority of MT-279 compound over 2-ETBI was identified in all forced swimming and rotarod tests.
Renal adenomatosis
Authors: Fronczek Agnieszka , Lewkowicz Dorota , Swatek Jaroslaw , Cieszczyk Katarzyna , Ciechan Janusz , Szumilo Justyna
Published: January 2018

Adenomatosis is a rare lesion of unknown etiology, defined as multiple (usually 5 or more) adenomas in one kidney. A case of renal adenomatosis in a 68-year-old woman treated previously for urolithiasis, who underwent nephrectomy because of the nonfunctional left kidney is reported. Apart from multiple adenomas, numerous hyperplastic lesions involving single tubules were present in the resected kidney. Both adenomas and hyperplastic lesions exhibited the expression of alpha-methylacyl-coenzyme A racemase (AMACR). Renal adenomatosis is worth special attention, since renal papillary adenomas are suggested as precursor lesions of papillary renal cell carcinoma that show similar AMACR expression.
Screening for Polyphenol Compounds and Antioxidant Capacity of Sweet Cherry Fruits Infected with Monilinia Laxa
Authors: Borković Boško , Malenčić Đorđe , Prvulović Dejan , Kiprovski Biljana , Stojšin Vera , Iličić Renata
Published: January 2018

Monilinia laxa Aderh. and Ruhl. is the predominant causal agent of brown rot disease of stone fruit orchards, especially sweet cherries. The objective of this study was to identify reaction in response of nine genotypes cherry, with different pomological properties, against brown rot. These genotypes were harvested at commercial maturity from orchard in the Fruit Research Institute in Rimski Šančevi. The studied genotypes showed significant differences in terms of the occurrence of disease on fruits, both under artificial inoculation and infection in the field. Given the fact that sweet cherry fruits are prone to infection by a number of pathogens in the field, biochemical parameters were analysed on artificially inoculated fruits. Biochemical analysis of fruits determined significant differences in contents of total phenols, flavonoids and anthocyanins, as well as in antioxidant activity. It was genotype specificities and intensity of infection, as well as the interaction of the two that induced differences in the secondary biomolecules content and antioxidant activity. The majority of the genotypes examined showed high polyphenolics content, while under the infection, the content was significantly lower. Based on the results obtained, the secondary metabolites content can be used as one of the parameters for evaluating the resistance of sweet cherry genotypes to brown rot.
The Role of Public-Private Partnerships in Higher Education How Tertiary Institutions in emerging Economies benefit from Public-Private Partnerships
Authors: Warasthe Ronak
Published: January 2018

The number of Public-Private Partnerships in the education sector is growing in developing and emerging economies. Traditionally governments are the main financial contributor to education however, the involvement of the private sector is an increasing one. While more established in primary and secondary education, PPPs in tertiary education are a phenomenon rather slowly growing in the past decades (Patrinos, Barrera-Osorio, & Guaqueta, 2009). There are various concepts of PPPs in higher education each targeting different goals. In order to give an insight into different types of PPPs, the typology according to Mabizela has been briefly displayed and the case of a PPP in Namibia is given. The framework of the partnership was compiled to give an outlook on the practicability of partnerships. The paper exemplifies that both partners within a PPP can benefit from the added value they may generate for their target group. Thus, the benefit depends on quality, relevance and execution of the partnership.
Job matching as a new challenge for work performance
Authors: Velciu Magdalena
Published: January 2018

In actual challenging environment, progress and growth is more than an economic issue and it involve human capital, development of human resources in terms of knowledge, skills, and competences. The workforce offer and demand is not sufficient to make progress but we need a concept that bring together skills, competency, jobs and productivity that drive economic growth. From the perspective of this paper, we deal with job mismatch as a qualitative imbalance that occurs when the characteristics of workers (level of education, qualification, skills) are not in direct relation with the work requirements, with accent on education requirements. I present some analytical highlights on education-job mismatch at European level and in Romania. Education requirements of jobs vary across countries. In Romania, almost 60% of adult employees, consider medium level of qualification is sufficient to do the job. Remarcable is that the procent is the highest amoung all responders from European countries. The subject have great importance to improve the matching as equilibrium between educational offer for developing skills, competences, knowledge with occupational requirements coming from changing business environment.
Research on the possibility of improving technical higher education through the parties involved in the Bologna Process
Authors: Oțel Călin Ciprian , Firescu Violeta
Published: January 2018

Through this paper, the authors have propose to briefly illustrate the shift from the “approach of knowledge as a product” to the “approach of knowledge as a process”, conducted through the Bologna Process in technical higher education. In this latter approach, the emphasis is on student rather than on teacher, as it is in the case of traditional higher education. The applied research was conducted at the Satu-Mare Branch of the Technical University of Cluj-Napoca through the project “Specialists for Satu-Mare” and the survey was used as method and the questionnaire as tool. The information gathered through the questionnaires distributed to both parties involved: students and teachers were analyzed. The aim of the research is to identify possible malfunctions and to propose recommendations for improving interactions in the educational process. The most important findings are: the need for the learning process to transfer especially practical knowledge and the promotion of “Professor Bologna's portrait” among teachers obtained as a result of applied research.
Challenges of scaling-up process for start-ups
Authors: Zajko Marian
Published: January 2018

Start-up is just a beginning phase of a company life cycle followed by the growth or scaling phase where the growth potential of start-up can develop into a high-growth company bringing revenues, well-paid jobs and taxes. The purpose of the paper is to analyse the essence of scaling up and the relevant external and internal factors for successful scaling up. The research method consists in analysis of internal and external conditions for successful scaling up and related support policies in the EU and Slovakia based on the secondary survey data and relevant publications. The findings about the Slovak start-up ecosystem confirm the relevance of risks of scaling up for the Slovak startups too. Therefore next to the improving of the start-up ecosystem the Slovak policy makers should develop systematic support measures for the scale-up ecosystem in Slovakia based on appropriate scale up metrics and incentives for support of start-ups in order to achieve a higher proportion of gazelles in the economy.
Branding in the age of social media: how entrepreneurs use social networks to boost their service-based businesses
Authors: Ioanid Alexandra , Deselnicu Dana Corina , Militaru Gheorghe
Published: January 2018

The basics of product and service branding are generally the same. However, creating a brand for a company that offers services requires more effort because the services are hard to visualize and each client has different expectations. More than promoting any of the services, branding aims to create a perception of trust so that the brand name to be associated with integrity, quality, and innovation. The purpose of this paper is to determine how entrepreneurs develop their service-based business through social networks branding and also what are the benefits obtained from each method. The authors have researched previously what is the impact of social network marketing on business development and noticed that service-based businesses tend to obtain benefits from different activities comparing to product-based businesses. The authors researched through a semistructured questionnaire the online activities of 71 SMEs from Bucharest that offer various types of services to their customers. The results obtained are then correlated with the online and offline performance of the company.
The Analysis and Improvement Strategies on College Students' Employment and Entrepreneurship Measures - Taking Universities in Hebei Province as an Example
Authors: Bai Haiqi , Han Mengxiao
Published: January 2018

Since the enrollment expansion of colleges and universities, University graduates have proliferated in China, the employment of college graduates is becoming more and more difficult. Real situation requires colleges and universities must spend big strength in starting a business to promote graduates employment. Based on colleges and universities to promote university students' employment entrepreneurship support measures as the research content, with in 5 colleges and universities in hebei province as the research object, through the investigation and study and literature analysis, the paper analyzes the problems existing in the guidance of employment and entrepreneurship, entrepreneurship education, school enterprise cooperation, employment. At the same time reference to the United Kingdom and the United States of college students employment experience, the paper put forward the improvement measures of the colleges and universities in the current stage to promote the employment and Entrepreneurship of College Students: equal emphasis on service and development in the field of employment guidance, the unity of theory and practice in the aspect of entrepreneurship education, in the aspect of school enterprise cooperation to achieve win-win.
Implementing A Low Cost Data Acquisition System for Engineering Education Programs in Universities
Authors: Rei Silviu
Published: January 2018

For many educational engineering projects running in Universities, a data acquisition system is a key component. Buying a system from the market is a viable option but many times the cost of it is prohibitive for the low budgets of University projects. This paper investigates the idea of implementing an own data acquisition system with use in small educational projects. Together with the advantage of cost reduction, an own implementation is an engineering exercise in itself, providing learning opportunities for the student. The paper presents first the results of a market scan for development platforms which can be used and then an example implementation of a simple data acquisition system using a low cost development platform. The performance of the implemented system is measured and presented. The main purpose of this paper is to encourage students and young researchers to implement their own tools, using this learning experiences along the way of meeting the project targets.
Integration of Entrepreneurship into Higher Education (Educational Sciences) in Lithuania and Latvia: Students and University Teachers’ Opinion
Authors: Melnikova Julija , Norbert Grünwald , Ahrens Andreas , Zaščerinska Jeļena
Published: January 2018

The purpose of this article is to highlight the aspects of integration of entrepreneurship into higher education (Educational sciences) in Lithuania and Latvia. The article examines the nature of challenges for entrepreneurial teaching and learning as well as provides some insights received form students and university teachers on how to improve the entrepreneurship-based teaching and learning in the universities of Lithuania and Latvia. The article describes the empirical study, which explored 32 student and 16 university lectures’ opinion on entrepreneurship in Educational sciences. The implications of the article could be useful for university educators, who seek to educate students more effectively with a real intention to become entrepreneurs.
Affective Iconoclasm: Codes of Labour as a Human Characteristic
Authors: Gronlund Melissa
Published: January 2018

This text argues that a number of recent works of contemporary art have developed an anthropomorphised code to signal “humanness.” Primary within this code is representations of labour, which the artworks connect to mimetic or realist stylisation as well as to the history of image production and often specifically Western art-making. It elaborates this thesis with regards to recent videos by Pierre Huyghe and Sidsel Meineche Hansen, and at a critique of social media labour in a lecture-performance by Jesse Darling, which all draw a link between human and non-human subjectivities and economic productivity. In focusing on different examples of nonhuman likenesses, the text also uses primatology to suggest that the colonial relationship between labour and species and racial hierarchies continues to colour representations of labour today.
To the Origins of American Archaeology in the Czech Lands: The Case of Julius Nestler
Authors: Křížová Markéta
Published: January 2018

The basis for the present article is the case study of Julius Nestler, amateur archaeologist from Prague, who at the beginning of the twentieth century pursued excavations in the ruins of Tiahuanaco and brought to Prague a unique collection of about 3,600 pieces, deposited now in the Náprstek Museum in Prague. His activities are put into the broader context of the origins of Americanist archaeology and anthropology in Central Europe, against a background of nationalist competition and economic entrepreneurship. The life story of Nestler also brings to the fore the problem of ethics in anthropological and archaeological work.
Clinical Characteristics of Ulceroglandular Tularemia in Two Bulgarian Regions, 2014-2015: a Report of Five Cases
Authors: Pekova Liliya M. , Baymakova Magdalena P. , Parousheva Pavlina O. , Fartunova Mariya D. , Dimitrov Nikolay E. , Tomova Iskra A.
Published: January 2018

We present here the first five human cases with tularemia from two regions in South Bulgaria in which there had been no previous report of the infection. The cases occurred over a period of 8 months (December 2014 - August 2015). They were treated at the Department of Infectious Diseases in Stara Zagora University Hospital, Bulgaria. We present the clinical, epidemiological and laboratory data for four men and one woman (age range 52 to 73 years). Three men were hunters, four patients took part in handling, preparing/skinning and cooking the game animals. One man marked agricultural work and contact with straw stems. After a mean incubation period of 4.8±1.4 days ulcers appeared, followed by local painful lymphadenitis. All patients presented with liver enlargement and elevation in acute phase reactants. The etiological diagnosis was made by tube agglutination test in all cases, PCR positive result was found in one. The administered antibacterial treatment was a combination of aminoglycosides and 4-quinolones with the outcome being favorable for all patients. The current report suggests presence of Francisella tularensis in South Bulgaria.
A Look at Global Climate Change Through Papal Encyclicals
Authors: Gutry-Korycka Małgorzata
Published: January 2018

The aim of this article is a comprehensive review of Papal Encyclicals in the context of global environmental and climatic change, against the backdrop of the activity of multinational institutions. The Encyclicals look to the future in teaching the faithful, in a manner which indicates that they are part of a goal-oriented policy, both in terms of scientific research, and concrete economic, social, and geopolitical activity. Attention has also been paid to the relationship between the activity of humankind, and global environmental change, particularly of the biotic and climatic variety. If this aggressive anthropogenic activity cannot be deemed responsible for initiating global warming, it may certainly be seen to have “encouraged” it. The impulses behind sustainable development, as well as the instruments of its implementation, and the inspiration behind the idea, have also been discussed. The achievement of this goal, necessitating the balancing of anthropological aspirations and the long-term security of the environment are also referenced in the Encyclicals.
A Postulate for Accelerated Implementation of Sustainable Development Goals and Principles
Authors: Kassenberg Andrzej
Published: January 2018

The last 30-year period has been increasingly important for sustainable development, as is evidenced by the UN’s announcement of 17 Sustainable Development Goals to be achieved by 2030. However, many megatrends continue to progress in the direction opposite to that meeting the needs of sustainable development. For the Earth and human civilisation to be protected, it is necessary for a new approach to be developed - a new order that might be termed the civilization/economics of enough, or of moderation. However, convincing societies, including that of Poland, in regard to the benefits of addressing the challenges sustainable development poses is not an easy task; even though a redirecting of the economy and of society along new paths will bring measurable benefits capable of compensating for the costs of transformation.
Effect of geometrical parameters on the effective elastic modulus for an X-type lattice truss panel structure
Authors: Zhang Qian , Jiang Wenchun , Zhang Yanting
Published: January 2018

The lattice truss panel structure (LTPS), which is a high strength material with high efficiency of heat transfer, has a good potential to be used as compact heat exchanger. The core of LTPS is a periodic porous structure, and the effective elastic modulus (EEM) will be different from the base material. It is essential to calculate the EEM for the design of this type of heat exchanger. This paper presents a study on the EEM of X-type LTPS by homogenization method, which has been verified by finite element method (FEM). It reveals that the effects of seven geometrical parameters of the X-type LTPS on EEM are not identical, and the relationship between the seven parameters and EEM has been established. Results calculated by homogenization method and FEM show a good agreement. The EEM decreases with the increase of truss length, stamping angle, shearing angle and node length, while it increases with the increase of truss width, truss thickness and face sheet thickness. Unlike the conventional foam material, there is no clear correlation between the EEM and the relative density, and a formula has been fitted to calculate the EEM of LTPS.
Inhibition of 5α-reductase alters pregnane metabolism in the late pregnant mare
Authors: Wynn Michelle A A , Ball Barry A , Legacki Erin , Conley Alan , Loux Shavahn , May John , Esteller-Vico Alejandro , Stanley Scott , Scoggin Kirsten , Squires Edward , Troedsson Mats
Published: January 2018

In the latter half of gestation in the mare, progesterone concentrations decline to near undetectable levels while other 5α-reduced pregnanes are elevated. Of these, 5α-dihydroprogesterone and allopregnanolone have been reported to have important roles in either pregnancy maintenance or fetal quiescence. During this time, the placenta is necessary for pregnane metabolism, with the enzyme 5α-reductase being required for the conversion of progesterone to 5α-dihydroprogesterone. The objectives of this study were to assess the effects of a 5α-reductase inhibitor, dutasteride on pregnane metabolism (pregnenolone, progesterone, 5α-dihydroprogesterone, 20α-hydroxy-5α-pregnan-3-one, 5α-pregnane-3β,20α-diol and allopregnanolone), to determine circulating dutasteride concentrations and to assess effects of dutasteride treatment on gestational parameters. Pregnant mares (n = 5) received dutasteride (0.01 mg/kg/day, IM) and control mares (n = 4) received vehicle alone from 300 to 320 days of gestation or until parturition. Concentrations of dutasteride, pregnenolone, progesterone, 5α-dihydroprogesterone, 20α-hydroxy-5α-pregnan-3-one, 5α-pregnane-3β,20α-diol, and allopregnanolone were evaluated via liquid chromatography–tandem mass spectrometry. Samples were analyzed as both days post treatment and as days prepartum. No significant treatment effects were detected in pregnenolone, 5α-dihydroprogesterone, 20α-hydroxy-5α-pregnan-3-one, 5α-pregnane-3β,20α-diol or allopregnanolone for either analysis; however, progesterone concentrations were increased (P < 0.05) sixfold in dutasteride-treated mares compared to control mares. Dutasteride concentrations increased in the treated mares, with a significant correlation (P < 0.05) between dutasteride concentrations and pregnenolone or progesterone concentrations. Gestational length and neonatal outcomes were not significantly altered in dutasteride-treated mares. Although 5α-reduced metabolites were unchanged, these data suggest an accumulation of precursor progesterone with inhibition of 5α-reductase, indicating the ability of dutasteride to alter progesterone metabolism.
PGRMC1 localization and putative function in the nucleolus of bovine granulosa cells and oocytes
Authors: Terzaghi Laura , Luciano Alberto Maria , Dall’Acqua Priscila C , Modina Silvia C , Peluso John J , Lodde Valentina
Published: January 2018

Progesterone receptor membrane component-1 (PGRMC1) is a highly conserved multifunctional protein that is found in numerous systems, including reproductive system. Interestingly, PGRMC1 is expressed at several intracellular locations, including the nucleolus. The aim of this study is to investigate the functional relationship between PGRMC1 and nucleolus. Immunofluorescence experiments confirmed PGRMC1’s nucleolar localization in cultured bovine granulosa cells (bGC) and oocytes. Additional experiments conducted on bGC revealed that PGRMC1 co-localizes with nucleolin (NCL), a major nucleolar protein. Furthermore, small interfering RNA (RNAi)-mediated gene silencing experiments showed that when PGRMC1 expression was depleted, NCL translocated from the nucleolus to the nucleoplasm. Similarly, oxidative stress induced by hydrogen peroxide (H2O2) treatment, reduced PGRMC1 immunofluorescent signal in the nucleolus and increased NCL nucleoplasmic signal, when compared to non-treated cells. Although PGRMC1 influenced NCL localization, a direct interaction between these two proteins was not detected usingin situproximity ligation assay. This suggests the involvement of additional molecules in mediating the co-localization of PGRMC1 and nucleolin. Since nucleolin translocates into the nucleoplasm in response to various cellular stressors, PGRMC1’s ability to regulate its localization within the nucleolus is likely an important component of mechanism by which cells response to stress. This concept is consistent with PGRMC1’s well-described ability to promote ovarian cell survival and provides a rationale for future studies on PGRMC1, NCL and the molecular mechanism by which these two proteins protect against the adverse effect of cellular stressors, including oxidative stress.
Effects of colostrum, feeding method and oral IGF1 on porcine uterine development
Authors: George Ashley F , Rahman Kathleen M , Miller Dori J , Wiley Anne A , Camp Meredith E , Bartol Frank F , Bagnell Carol A
Published: January 2018

Nursing ensures lactocrine delivery of maternally derived, milk-borne bioactive factors to offspring, which affects postnatal development of female reproductive tract tissues. Disruption of lactocrine communication for two days from birth (postnatal day (PND) 0) by feeding milk replacer in lieu of nursing or consumption of colostrum alters porcine uterine gene expression globally by PND 2 and inhibits uterine gland genesis by PND 14. Here, objectives were to determine effects of: (1) nursing or milk replacer feeding from birth; (2) a single dose of colostrum or milk replacer and method of feeding and (3) a single feeding of colostrum or milk replacer, with or without oral supplementation of IGF1, administered at birth on aspects of porcine uterine development at 12-h postnatally. Results indicate nursing for 12 h from birth supports rapid establishment of a uterine developmental program, illustrated by patterns of endometrial cell proliferation, expression of genes associated with uterine wall development and entry into mitosis and establishment of a uterine MMP9/TIMP1 system. A single feeding of colostrum at birth increased endometrial cell proliferation at 12 h, regardless of method of feeding. Oral supplementation of IGF1 was sufficient to support endometrial cell proliferation at 12 h in replacer-fed gilts, and supplementation of colostrum with IGF1 further increased endometrial cell proliferation. Results indicate that lactocrine regulation of postnatal uterine development is initiated with the first ingestion of colostrum. Further, results suggest IGF1 may be lactocrine-active and support a 12-h bioassay, which can be used to identify uterotrophic lactocrine activity.
Genomic Identification and Functional Characterization of Essential Genes in Caenorhabditis elegans
Authors: Qin Zhaozhao , Johnsen Robert , Yu Shicheng , Chu Jeffrey Shih-Chieh , Baillie David L , Chen Nansheng
Published: January 2018

Using combined genetic mapping, Illumina sequencing, bioinformatics analyses, and experimental validation, we identified 60 essential genes from 104 lethal mutations in two genomic regions of Caenorhabditis elegans totaling ∼14 Mb on chromosome III(mid) and chromosome V(left). Five of the 60 genes had not previously been shown to have lethal phenotypes by RNA interference depletion. By analyzing the regions around the lethal missense mutations, we identified four putative new protein functional domains. Furthermore, functional characterization of the identified essential genes shows that most are enzymes, including helicases, tRNA synthetases, and kinases in addition to ribosomal proteins. Gene Ontology analysis indicated that essential genes often encode for enzymes that conduct nucleic acid binding activities during fundamental processes, such as intracellular DNA replication, transcription, and translation. Analysis of essential gene shows that they have fewer paralogs, encode proteins that are in protein interaction hubs, and are highly expressed relative to nonessential genes. All these essential gene traits in C. elegans are consistent with those of human disease genes. Most human orthologs (90%) of the essential genes in this study are related to human diseases. Therefore, functional characterization of essential genes underlines their importance as proxies for understanding the biological functions of human disease genes.
Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish
Authors: Chen Tianbing , Song Guili , Yang Huihui , Mao Lin , Cui Zongbin , Huang Kaiyao
Published: January 2018

Hermansky-Pudlak syndrome (HPS) is a human autosomal recessive disorder that is characterized by oculocutaneous albinism and a deficiency of the platelet storage pool resulting from defective biogenesis of lysosome-related organelles (LROs). To date, 10 HPS genes have been identified, three of which belong to the octamer complex BLOC-1 (biogenesis of lysosome-related organelles complex 1). One subunit of the BLOC-1 complex, BLOS1, also participates in the BLOC-1-related complex (BORC). Due to lethality at the early embryo stage in BLOS1 knockout mice, the function of BLOS1 in the above two complexes and whether it has a novel function are unclear. Here, we generated three zebrafish mutant lines with a BLOC-1 deficiency, in which melanin and silver pigment formation was attenuated as a result of mutation of bloc1s1, bloc1s2, and dtnbp1a, suggesting that they function in the same complex. In addition, mutations of bloc1s1 and bloc1s2 caused an accumulation of clusters of lysosomal vesicles at the posterior part of the tectum, representing a BORC-specific function in zebrafish. Moreover, bloc1s1 is highly expressed in the swimbladder during postembryonic stages and is required for positively regulating the expression of the genes, which is known to govern surfactant production and lung development in mammals. Our study identified BLOS1 as a crucial regulator of the surfactant system. Thus, the zebrafish swimbladder might be an easy system to screen and study genetic modifiers that control surfactant production and homeostasis.
Rhinovirus in Febrile Infants and Risk of Bacterial Infection
Authors: Blaschke Anne J. , Korgenski E. Kent , Wilkes Jacob , Presson Angela P. , Thorell Emily A. , Pavia Andrew T. , Knackstedt Elizabeth D. , Reynolds Carolyn , Schunk Jeff E. , Daly Judy A. , Byington Carrie L.
Published: January 2018

BACKGROUND:

Febrile infants with viral respiratory infections have a reduced risk of bacterial infection compared with virus-negative infants. The risk of concomitant bacterial infection in febrile infants positive for human rhinovirus (HRV) by polymerase chain reaction (PCR) is unknown.

METHODS:

Infants 1–90 days old managed using the care process model for well-appearing febrile infants and with respiratory viral testing by PCR (RVPCR) in the emergency department or inpatient setting of 22 hospitals in the Intermountain Healthcare system from 2007-2016 were identified. Relative risk (RR) of bacterial infection was calculated for infants with HRV, non-HRV viruses, or no virus detected.

RESULTS:

Of 10 964 febrile infants identified, 4037 (37%) had RVPCR. Of these, 2212 (55%) were positive for a respiratory virus; 1392 (35%) for HRV alone. Bacterial infection was identified in 9.5%. Febrile infants with HRV detected were more likely to have bacterial infection than those with non-HRV viruses (7.8% vs 3.7%; P < .001; RR 2.12 [95% CI 1.43–3.15]). Risk of urinary tract infection was not significantly different for HRV-positive infants at any age, nor was risk of invasive bacterial infection (IBI; bacteremia and/or meningitis) meaningfully different for infants 1–28 day olds. Infants 29–90 days old with HRV had a decreased likelihood of IBI (RR 0.52 [95% CI 0.34–0.80]).

CONCLUSIONS:

HRV is common in febrile infants. Detection did not alter risk of concomitant urinary tract infection at any age or risk of IBI in infants 1–28 days old. HRV detection may be relevant in considering risk of IBI for infants 29–90 days of age.
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
Authors: Crespo-Muñoz Sergio , Rivera-Peña Mariela , Rosero-Alpala Deisy Alexandra , Muñoz-Florez Jaime Eduardo , Rao Idupulapati M. , Muñoz-Florez Ligia Carmenza
Published: January 2018

El fríjol tépari, Phaseolus acutifolius A. Gray destaca por su tolerancia al estrés por sequía y altas temperaturas, sin embargo, presenta baja diversidad genética en sus formas cultivadas. Por lo que se inició un programa de inducción de mutaciones. El objetivo de este estudio fue establecer el efecto de diferentes parámetros  agro-morfofisiológicos asociados sobre la viabilidad y cantidad del polen en 123 líneas mutantes de la quinta generación M5 de P. acutifolius y genotipos parentales, por medio del procesamiento de microfotografías de preparaciones de polen con tinción de acetocarmin. El experimento se realizó en condiciones de invernadero y el diseño experimental fue de subparcelas divididas con 3 repeticiones; adicionalmente, los factores evaluados fueron: condiciones de humedad del suelo, inoculación con cepas de Bradyrhizobium sp. o Rhizobium tropici, y las líneas o accesiones evaluadas (6 mutantes y 2 no mutantes). El experimento fue realizado manteniendo la temperatura nocturna superior a 25°C. No hubo diferencias significativas entre los tratamientos, se encontraron correlaciones positivas de la viabilidad de polen con otras variables.
Assessment of 112 tomato (Solanum lycopersicum L.) cultivars for industrial processing in Portoviejo, Ecuador
Authors: Cañadas-López Alvaro Gustavo , Rade-Loor Diana Yasbhet , Quijije-Pinargote Raúl Ovidio , Sotomayor Ignacio Antonio , Ormaza-Molina Alexandra Maricela
Published: January 2018

La industria de procesamiento de tomate (Solanum lycopersicum L.) requiere cultivares con producción y calidad superiores, además de resistencia a plagas, para que los productores prefieran sembrar estos cultivares. La presente investigación se efectuó con el objetivo de evaluar 112 materiales en relación a parámetros de producción y calidad, provenientes de los siguientes proveedores: Orsetti Seed, Heiz Seed, Ohio University, United Genetic y Harrys Moran. El experimento se realizó en la Estación Experimental Portoviejo del INIAP. Para el análisis estadístico se emplearon metodología de análisis de componentes principales para la agrupación de 112 cultivares basados en parámetros de producción como: rendimiento (t.ha-1), peso promedio de fruto (g), número de frutos por plantas, número de frutos defectuosos; y además los parámetros de calidad son: grados brix, pH y acidez. Los datos se analizaron mediante análisis de varianza y pruebas de Tukey para establecer diferencias estadísticas y rango de significancia entre tomates industriales. Los resultados mostraron que cerca del 9 % de los materiales evaluados presentaron características superiores. Cinco rangos de significancia estadística se encontraron, donde los tres primeros fueron representados por los materiales desarrollados por la Universidad de Ohio. El mejor material evaluado fue el cultivar SG 07-627, y todos los materiales presentaron distintivos de resistencias a plagas, con potencial para el mejoramiento genético como parte de la estrategia de manejo integral de plagas en tomate.
Characterization and mapping of soil classes of the Lajeado Pessegueiro watershed in Santa Catarina, Brazil
Authors: Machado Ludmila Nascimento , Loss Arcangelo , Bacic Ivan Luiz Zilli , Dortzbach Denilson , Lalane Heloísa De campos
Published: January 2018

Los estudios pedológicos y la caracterización fisiográfica de una cuenca son procedimientos fundamentales para planificar el uso del suelo. Por lo tanto, el objetivo de este estudio fue caracterizar y mapear las clases de suelos de la cuenca Lajeado Pessegueiro, ubicada en Guarujá do Sul, Santa Catarina, Brasil. Se delinearon y describieron los tipos de uso y ocupación de la tierra, las clases de pendiente y la fisiografía del área de estudio.Los datos se obtuvieron de 19 perfiles de suelo completos y 9 muestras adicionales. El análisis fisiográfico se realizó con información previa sobre la fotopedología de las características geomorfológicas del terreno, las condiciones climáticas, la vegetación, el uso de la tierra y las condiciones geológicas. La cuenca tiene un relieve predominantemente ondulado (406.51 ha). El uso predominante de la tierra es agrícola, con cultivos anuales temporales (50.09%). La fisiografía predominante es la pendiente erosiva coluvial (35.53%), seguida por la pendiente erosiva (27.13%), la pendiente de la meseta (21.55%), el fondo del valle coluvial aluvial (10.51%) y la pendiente coluvial erosiva (5.23%). Las clases de suelos encontradas fueron Nitosoles Rojos (37.54%), Nitosoles Hálpidos (8.22%), Cambisoles Hálpidos (35.20%), Cambisoles Fluviales (3.92%), Argisoles Rojos (10.09%) y Neosoles Lithólicos (5.04%). Los datos mostraron la importancia de utilizar el análisis fisiográfico del área con técnicas de geoprocesamiento, ayudando a las encuestas pedológicas con la presentación a través de mapas temáticos. Estos mapas sirven como material base en estudios que evalúan los usos del suelo y aquellos que apuntan a contribuir a una mejor planificación del uso del suelo.
Seedlings production of two tomato (Solanum licopersicum L.) cultivars under different environments and substrates
Authors: Lopes-Monteiro-Neto João Luiz , Da Silva-Siqueira Raphael Henrique , De Oliveira-Nunes Taline Katlen , Soares da Silva Edgley , Da Silva- Maia Sonicley , Farias-Araújo Wellington , Alves -de Albuquerque José De Anchieta , Oliveira-Vilarinho Lucianne Braga , Alves-Chagas Edvan , Abanto-Rodriguez Carlos
Published: January 2018

El tipo de ambiente y la calidad de los sustratos en la producción de plántulas son esenciales para la producción de plantas productivas bajo condiciones de campo. Con el objetivo de determinar el mejor ambiente y sustrato para la producción de plántulas de dos cultivares de tomate (IPA 06 y Santamélia), dos experimentos, completamente aleatorios y dispuestos en parcelas subdivididas con cinco repeticiones, se llevaron a cabo bajo las condiciones ambientales de Boa Vista, Roraima, Brasil . Los tratamientos consistieron en la asociación entre cinco ambientes (A1 - invernadero, A2 - Chromatinet ® 35% rojo, A3 - Chromatinet ® 50% rojo, A4 - Chromatinet ® 35% plata y A5 - Chromatinet ® 50% plata) y cuatro sustratos S1 - OrganAmazon®; S2 - OrganAmazon® + PureHumus®; S3 - OrganAmazon® + PureHumus® + estiércol del suelo + bovino, y S4- OrganAmazon® + PureHumus® + suelo + estiércol + cascarilla de arroz carbonizada (CRH) en las siguientes variables: número de hojas, altura de la planta, diámetro del tallo, brote seco y la biomasa de la raíz, la relación entre la altura de la planta y el diámetro del tallo de acuerdo con el índice de calidad de las plántulas de colon y Dickson. Los sustratos se hicieron con la adición de PureHumus®, sin presentar CRH (S2 y S3), lo que favoreció el aumento de todas las variables analizadas. Además, el invernadero, especialmente cuando está asociado al sustrato S2, había logrado el mejor ambiente para la producción de plántulas de los dos cultivares de tomate evaluados. Las redes de sombreado fotoconvertidor no fueron eficientes en la producción de plántulas de tomate en condiciones climáticas de Boa Vista, Roraima, Brasil.
Simón Puerta Domínguez, Cine y nación. Negociación, construcción y representación identitaria en Colombia. Medellín: Universidad de Antioquia, 2015, 243 pp.
Authors: Correa Serna Nancy Yohana
Published: January 2018

Reseña de Simón Puerta Domínguez, Cine y nación. Negociación, construcción y representación identitaria en Colombia. Medellín: Universidad de Antioquia, 2015, 243 pp.
Size optimization of shoulder filleted shafts with relief grooves for improving their fatigue life
Authors: González-Mendoza Juan Manuel , Alcántara-Montes Samuel , Silva-Lomelí José De Jesús , De la Cruz-Alejo Carlos , Ocampo-Ramírez Arturo
Published: January 2018

Although in scientific literature there are studies regarding the inclusion of relief grooves in order to diminish the amount of stress concentration in stepped shafts, the incorporation of optimization algorithms capable of parametrically determining their geometry remains unexplored. In this paper, an approach to the problem of size optimization of shoulder filleted shafts with relief grooves and subject to axial loads is presented. The objective of the optimization is to minimize the maximum value of stress at both, the root of the shoulder fillet, and the root of the groove, thus minimizing stress concentration and improving fatigue life of such elements. Under this methodology, different percentages of reduction of stress are achieved for the shafts with relief grooves, in comparison with the shafts without relief grooves. The novelty of this approach lies in the incorporation of an algorithm for the determination of the optimum geometry of the grooves.