bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Environmental Performance dan Corporate Social Responsibility terhadap Kinerja Perusahaan Manufaktur yang Terdaftar di BEI Tahun 2018-2022
Authors: Husda Anggun Permata , Azmiana Risca , Banjarnahor Haposan
Published: October 2023

The research aims to analyze environmental performance and corporate social responsibility on the Performance of Manufacturing Companies listed on the IDX in 2018-2022. This research uses 34 mining companies listed on the IDX in 2018-2022. The sampling technique used purposive sampling. The sample used was 12 coal mining companies listed on the Indonesia Stock Exchange. The data used is in the form of annual reports and sustainability reports which are reported consistently every year by the company. Process research data using SPSS 29 for partial test (t) and simultaneous test (f). The results of the study show that environmental performance has a significant positive effect on the performance of mining companies listed on the IDX in 2018-2022. Then Corporate Social Responsibility has a significant positive effect on the performance of mining companies listed on the IDX in 2018-2022. Environmental performance and corporate social responsibility simultaneously have a significant positive effect on the performance of mining companies listed on the IDX in 2018-2022.
Pengaruh Gaya Kepemimpinan Dan Kompetensi Pegawai Terhadap Produktivitas Kerja Pada PT Pos Indonesia Di Kota Batam
Authors: Wasiman Wasiman , Wangdra Yvonne
Published: October 2023

Style of leadership is the norm of behavior used by a person when the person is trying to influence the behavior of others as he sees. The term competence lies in part in every human being and forever exist in a person's personality can predict the behavior and performance broadly in all situations and work tasks or jobs task. Meanwhile, pengerak Increased productivity is a motor of economic progress and benefit the company or organization. Productivity is also important to increase wages and individual acceptance. A country that is unable to increase its productivity will decline in the standard of life. Methods: Total population in this study is 564 people and the amount of sample in this study is 234 people, with the Data Collection Techniques questionnaire, sampling technique stratified Sampling.Analisis research using the technique used in this research is multiple linear regression analysis that has a purpose to determine the magnitude of the regression coefficients which will show the level of influence of independent variables on the dependent variable. T test results showed that leadership style variable (X1) significantly affects the Work Productivity (Y) and Employee Competency variables (X2) significantly affects the Work Productivity (Y). F-test results showed that the Leadership Style (X1) and Employee Competence (X2) effect simultaneously on Work Productivity (Y) at PT Pos Indonesia in Batam.
Pembinaan Sistem Akuntansi Serta Pelaporan Keuangan di SMK Kolese Tiara Bangsa
Authors: Effendi Syahril , Tukino Tukino , Wangdra Ronald , Wangdra Yvonne
Published: October 2023

Effective accounting and financial reporting systems are crucial for ensuring transparency, accuracy, and the sustainability of finances in vocational schools like SMK Kolese Tiara Bangsa. This research aims to examine the efforts in fostering and enhancing the accounting and financial reporting systems at SMK. The study involves data collection from various sources, including interviews with administrative staff, document analysis, and monitoring of accounting procedures. The research findings indicate that SMK Kolese Tiara Bangsa has undertaken significant efforts to improve their financial governance. These efforts include training for administrative staff, the utilization of state-of-the-art accounting software, and enhancing understanding of applicable accounting standards. Furthermore, the school has also revamped their financial reporting processes, encompassing the regular preparation of financial statements and strict budget monitoring. The improvement in the accounting and financial reporting system has aided SMK Kolese Tiara Bangsa in more effectively managing their finances, enhancing the accuracy of financial information, and increasing transparency and accountability. This study provides valuable insights into the importance of fostering and developing accounting systems in educational institutions, with the hope of setting an example for similar institutions aiming to improve their financial management.
Analisis Efektifitas Dan Kontribusi Penerimaan Pajak Parkir Terhadap Pendapatan Asli Daerah Di Kota Batam
Authors: Effendi Syahril
Published: October 2023

The aim of this research is to find answers to determine the effectiveness of parking taxes and their contribution to regional revenue. In the era of regional autonomy, local governments are required to continuously increase their regional income, such as by boosting local tax revenue through parking taxes. Revenue generated from parking taxes in the city of Batam makes a substantial contribution to regional income. However, in practice, it has not fully reached its true potential. Additionally, the formulation of tax targets is based solely on past years' experiences. This research was conducted from April to July 2018 and focused on the Tax and Revenue Office of Batam City. The sampling technique used was non-probability sampling, specifically purposive sampling, using financial reports from 2018 to 2022. The results of the hypothesis testing using multiple regression analysis indicate that, simultaneously, the effectiveness of parking taxes and the contribution of parking taxes significantly affect regional revenue. However, individually, the effectiveness of parking taxes has a significant relationship with regional revenue, while the contribution of parking taxes also has a significant impact on regional revenue in the Tax and Revenue Office of Batam City.
Pengaruh Pendapatan Asli Daerah (PAD) Dan Dana Perimbangan Terhadap Belanja Daerah Di Indonesia
Authors: Wage Sunarto , Effendi Syahril
Published: October 2023

This research aims to analyze and find out how much influence regional original income (PAD) and balancing funds have on regional expenditure in Indonesia. Research data comes from the Central Statistics Agency in 34 Provinces in Indonesia for the period 2018 - 2021 with 136 data. After data collection, there were several provinces in Indonesia whose data were incomplete so the data used was 100. The provinces with incomplete data were: Bengkulu, Bali, South Kalimantan, Central Kalimantan, West Nusa Tenggara, South Sulawesi, West Sulawesi, Main Maluku and West Papua. Data analysis techniques use (1) Classic assumption tests, namely normality, heteroscedasticity, multicollinearity and autocorrelation tests, (2) Multiple linear regression analysis, t test, F test and coefficient of determination. The results of the research show that partially original regional income has no effect on regional spending in Indonesia, balancing funds have no effect on regional spending in Indonesia, and simultaneously original regional income and balancing funds have no effect on regional spending in Indonesia.
Overview Terhadap Faktor Yang Mempengaruhi Implementasi Pengembangan Pariwisata Di Kepulauan Anambas
Authors: Khairina Etika , Kurnia Renita , Zukriadi Diki , Lebang M. Andika Surya , Jamba Padrisan
Published: October 2023

This research aims to analyze the factors that influence the implementation of tourism development in the Anambas Islands to increase the number of tourist visits. The research method is descriptive qualitative, primary data sources are interviews and observations. Secondary resources are reviews of relevant literature and documentation from the Anambas Cultural Tourism Office. The data analysis technique is Triangulation. Factors influencing implementation can be seen from the theory developed by Van Metter and Van Horn, namely policy standards and targets, resources, communication, characteristics of implementing agents, and social, political, and economic conditions. The regulations governing Anambas Islands tourism are included in the National Tourism Destination (DPN) development plan through the 2010-2025 National Tourism Development Master Plan (RIPPARNAS), which is directed at tourism development which is difficult to achieve policy program targets, this is because in the regulations There are no Standard Operating Procedures available in tourism development itself, so that SOPs in regulations become one of the influential obstacles. Then, synergy between the government and the community is needed in implementing the tourism development of the Anambas Islands.
Tingkat Partisipasi Masyarakat Akan Kebijakan Pelaksanaan Vaksinasi Covid-19 Di Kota Batam
Authors: Permatasari Denti , Dompak Timbul
Published: October 2023

The objective of this study is to assess the extent of community engagement in the execution of the Covid-19 vaccination strategy within Batam City. The administration of Covid-19 vaccines plays a pivotal role in surmounting a worldwide pandemic, with active engagement from the community serving as a significant determinant of the efficacy of a vaccination initiative. The present study employs a qualitative methodology for data collection. A survey utilizing a structured questionnaire will be administered to assess the degree of community engagement in the Covid-19 vaccination process. Furthermore, a comprehensive analysis will be undertaken through conducting in-depth interviews with a carefully selected group of respondents. This approach aims to acquire a more profound comprehension of the various factors that exert influence on their level of participation. The anticipated outcomes of this research endeavor aim to offer a comprehensive assessment of the extent of community engagement in the execution of the Covid-19 vaccination program within Batam City. The aforementioned findings will offer significant insights to authorities and healthcare providers, enabling them to formulate enhanced communication and outreach strategies aimed at augmenting public engagement in vaccination programs.
Pelayanan Pemerintah Berbasis Konsep Smart City Studi Kasus : Pelayanan Pemerintah Kota Yogyakarta berbasis Smart Environment
Authors: Salsabila Lubna
Published: October 2023

The smart environmental policy of the city of Yogyakarta as one aspect of the smart city is the focus of research. Apart from that, how the government can implement green waste as an aspect of the smart environment and use qualitative descriptive research methods. The research results reveal that the Yogyakarta City Government in running its government and serving its people is in accordance with the indicators of the smart environment concept. This means that the government's efforts to implement the program have been successful and comprehensive. The program covers 3 aspects of smart environmental indicators, namely green planning and green design, green open space and green waste. For the waste management program, the Yogyakarta city government is considered successful in reducing waste and managing its waste, this is proven by the innovation of the Waste Bank as a solution to the large amount of waste in the city. from Yogyakarta. However, of course the implementation of smart city in the city of Yogyakarta must also continue to develop and be sustainable so that the performance of the Yogyakarta city government must be improved and the active participation of the people of Jogjakarta to create a new innovation to be able to realize a smart city that fulfills all aspects other than the smart environment aspect.
Pertanggungjawaban Pidana Dalam Penyebaran Data Pribadi: Tinjauan Hukum Pidana Saat Ini
Authors: Jamba Padrisan , Svinarky Irene
Published: October 2023

In an increasingly digital era, the issue of theft and dissemination of personal data has become a profound global concern. This research focuses on criminal liability in the dissemination of personal data, with the aim of reviewing and analyzing the current criminal legal framework in addressing violations related to personal data. The research employs a normative juridical method, referencing various legislations, court decisions, and relevant legal literature. We also analyze recent changes and developments in criminal law concerning the dissemination of personal data. The results of this research depict the complexity of regulations related to handling cases of personal data dissemination. Factors such as the type of data involved, the intent of the perpetrator, and the impact on affected individuals are crucial considerations in determining the level of criminal liability. The research also discusses potential criminal sanctions that may be imposed on perpetrators of personal data breaches, including fines and imprisonment. Additionally, we identify challenges in enforcing laws related to personal data and provide insights into potential improvement efforts. This research provides valuable insights into how current criminal law addresses the issue of personal data dissemination and promotes better protection for individuals in an ever-evolving digital era.
Pembelajaran Sains Melalui Mini Praktikum untuk Anak Usia Dini dan Usia Sekolah di Rumah Pintar Kreatif Ampang, Kelurahan Ampang, Sumatera Barat
Authors: Yansen Fatridha , Mossfika Eldya , Elfia Linda
Published: October 2023

Science has an important role in improving the quality of human life and has significant positive effects on the development of children’s personality and potential. However, many people believe that science is a challenging field of study. Therefore, it is important to introduce science to children at a young age in an entertaining way. This community service activity aims to introduce science to early childhood and increase children's abilities and interest in science. The target of this activity is children from middle-class to lower-class families that live in the area of the  Rumah Pintar Mingguan (RPM) Kreatif Ampang. Five RPM volunteers and 31 children participated in this activity. This PKM activity was carried out through a simple science mini-practicum, by utilizing simple and inexpesive materials and tools that can be found easily in the surrounding environment. The main ingredients were baking soda and vinegar (acetic acid). The method used in this activity were a case study and hands-on practice. Two topics that are carried out on this practicum, namely the phenomenon of an erupting Merapi volcano and inflating a balloon without being blown up. This practice has the effect of increasing children's interest in and motivation for science, which is directly correlated with their increased comprehension of the concepts being demonstrated. Additionally, RPM decided to make this science practicum activity as a regular agenda item for the RPM community.
Pelatihan Peningkatan Usaha Pedagang Asongan Di Perumahan Buana Impian Kota Batam
Authors: Hakim Arif Rahman , Putria Narti Eka , Azwanti Nurul
Published: October 2023

This activity aims to enhance the businesses of street vendors in Buana Impian Housing Complex 1 & 2, particularly those selling various goods. Initially, Buana 1 & 2 had a single RW 014, but in 2016, Buana Impian 2 became RW 028. Along the streets of Buana Impian Housing Complex 1 & 2, there are shops and a total of 53 street vendors. As part of this Community Service Program (PKM), the street vendors will receive training and guidance through lectures and discussions addressing the challenges faced by street vendors in Buana Impian Housing Complex 1 & 2. The educational and training materials will cover business management and entrepreneurship, accounting training for income and expenses, strategies for promoting businesses, and access to funding opportunities for street vendors. By participating in this training and education program, it is expected that the businesses of the street vendors will thrive and flourish.
Social media attacks against female Canadian journalists
Authors: Al-Rawi Ahmed
Published: October 2023

I investigate in this brief empirical study the social media attacks against female Canadian journalists who have frequently been targeted with online abuse. I used purposive sampling to focus on three journalists: Rachel Gilmore (formerly with Global News), Erica Iffil (freelance with The Hill Times), and Saba Eitizaz (Toronto Star). I employed a mixed method approach to conduct this study by collecting all the available Twitter replies to these three journalists (n = 402,821) posted by 84,962 unique users. The digital analysis results show that there are slight differences in the quantity of attacks on these journalists, but the qualitative assessment of images associated with tweets indicate the need to use manual approaches to better understand the nuances and quality of these disinformation and often racist attacks.
Feasibility of gray-blood late gadolinium enhancement evaluation in young patients with congenital and acquired heart disease
Authors: Gonzalez de Alba Cesar , Moghari Mehdi H. , Browne Lorna P. , Friesen Richard M. , Fonseca Brian , Malone LaDonna J.
Published: October 2023

Background

Late gadolinium enhancement (LGE) sequences have become common in pediatric cardiovascular magnetic resonance (CMR) to assess for myocardial fibrosis. Bright-blood late gadolinium enhancement (BB-LGE) by conventional phase-sensitive inversion recovery (PSIR) is commonly utilized, but similar inversion time (TI) value of fibrosis and left ventricular (LV) blood pool can make subendocardial areas difficult to assess. A gray-blood LGE (GB-LGE) technique has been described, targeting nulling of the LV blood pool and demonstrating improvement in ischemic scar detection over BB-LGE in adult patients. We sought to evaluate the feasibility of the GB-LGE technique in a young population with congenital and acquired heart disease and compare its ability to detect subendocardial scar to conventional BB-LGE.

Methods

Seventy-six consecutive patients referred for clinical CMR underwent both BB-LGE and GB-LGE on 1.5 T and 3 T scanners. Conventional PSIR sequences were obtained with TI to null the myocardium (BB-LGE) in short-axis and horizontal long-axis stacks. Same PSIR stacks were immediately repeated with TI to null the blood pool (GB-LGE). Both sequences were reviewed separately a week apart by two readers, blinded to the initial clinical interpretation. Studies were analyzed for overall image quality, confidence in scar detection, confidence in detection of LGE, LGE class, inter- and intra-observer agreement for the presence of scar, and intraclass correlation coefficient (ICC) for total scar burden.

Results

Overall confidence in myocardial scar detection by BB-LGE or GB-LGE as well as grading of image quality were not statistically different [(p = 1 and p = 1) and (p = 0.53, p = 0.18), respectively]. There was very good inter-observer agreement for the presence of scar on BB-LGE (K = 0.88, 95% CI 0.77–0.99) and GB-LGE (K = 0.84, 95% CI 0.7–0.96), as well as excellent intra-observer agreement for both readers (K = 0.93, 95% CI 0.87–0.99; and K = 0.81, 95% CI 0.69–0.95). Interclass correlation coefficient for total scar burden was excellent for BB-LGE (ICC = 0.98, 95% CI 0.96–0.99) and GB-LGE (ICC = 0.94, 95% CI 0.91–0.97).

Conclusions

The GB-LGE technique is feasible in the pediatric population with congenital and acquired heart disease. It can detect subendocardial/ischemic scar similar to conventional bright-blood PSIR sequences in the pediatric population.
Analysis of copy number variations and possible candidate genes in spontaneous abortion by copy number variation sequencing
Authors: Bai Wei , Zhang Qi , Lin Zhi , Ye Jin , Shen Xiaoqi , Zhou Linshuang , Cai Wenpin
Published: October 2023

Introduction

Embryonic chromosomal abnormalities represent a major causative factor in early pregnancy loss, highlighting the importance of understanding their role in spontaneous abortion. This study investigates the potential correlation between chromosomal abnormalities and spontaneous abortion using copy number variation sequencing (CNV-seq), a Next-Generation Sequencing (NGS) technology.

Methods

We analyzed Copy Number Variations (CNVs) in 395 aborted fetal specimens from spontaneous abortion patients by CNV-seq. And collected correlated data, including maternal age, gestational week, and Body Mass Index (BMI), and analyzed their relationship with the CNVs.

Results

Out of the 395 cases, 67.09% of the fetuses had chromosomal abnormalities, including numerical abnormalities, structural abnormalities, and mosaicisms. Maternal age was found to be an important risk factor for fetal chromosomal abnormalities, with the proportion of autosomal trisomy in abnormal karyotypes increasing with maternal age, while polyploidy decreased. The proportion of abnormal karyotypes with mosaic decreased as gestational age increased, while the frequency of polyploidy and sex chromosome monosomy increased. Gene enrichment analysis identified potential miscarriage candidate genes and functions, as well as pathogenic genes and pathways associated with unexplained miscarriage among women aged below or over 35 years old. Based on our study, it can be inferred that there is an association between BMI values and the risk of recurrent miscarriage caused by chromosomal abnormalities.

Discussion

Overall, these findings provide important insights into the understanding of spontaneous abortion and have implications for the development of personalized interventions for patients with abnormal karyotypes.
Novel predictors for livebirth delivery rate in patients with idiopathic non-obstructive azoospermia based on the clinical prediction model
Authors: Zhang Li , Wang Yuan-yuan , Zheng Xiao-ying , lei Li , Tang Wen-hao , Qiao Jie , Li Rong , Liu Ping
Published: October 2023

Objective

To build a prediction model for live birth delivery per intracytoplasmic sperm injection (ICSI) in iNOA patients by obtaining sperm by microdissection testicular sperm extraction (mTESE).

Methods

A retrospective cohort study of 377 couples with iNOA male partners treated with 519 mTESE-ICSI cycles was conducted from September 2013 to July 2021 at the Reproductive Medical Centre of Peking University Third Hospital. Following exclusions, 377 couples with iNOA male partners treated with 482 mTESE-ICSIs were included. A prediction model for live birth delivery per ICSI cycle was built by multivariable logistic regression and selected by 10-fold cross-validation. Discrimination was evaluated by c-statistics and calibration was evaluated by the calibration slope.

Results

The live birth delivery rate per mTESE-ICSI cycle was 39.21% (189/482) in these couples. The model identified that the presence of motile sperm during mTESE, bigger testes, higher endometrial thickness on the day of human chorionic gonadotrophin (hCG) administration (ET-hCG), and higher quality embryos are associated with higher live birth delivery success rates. The results of the model were exported based on 10-fold cross-validation. In addition, the area under the mean ROC curve was 0.71 ± 0.05 after 10-fold cross-validation, indicating that the prediction model had certain prediction precision. A calibration plot with an estimated intercept of -1.653 (95% CI: -13.403 to 10.096) and a slope of 1.043 (95% CI: 0.777 to 1.308) indicated that the model was well-calibrated.

Conclusion

Our prediction model will provide valuable information about the chances of live birth delivery in couples with iNOA male partners who have a plan for mTESE-ICSI treatment. Therefore, it can improve and personalize counseling for the medical treatment of these patients.
Exploring the mechanism of traditional Chinese medicine in regulating gut-derived 5-HT for osteoporosis treatment
Authors: Sun Kai , Wang Yincang , Du Jiazhe , Wang Yujie , Liu Bo , Li Xiaodong , Zhang Xiaofeng , Xu Xilin
Published: October 2023

Osteoporosis is a systemic bone disease characterized by an imbalance in the relationship between osteoblasts, osteocytes, and osteoclasts. This imbalance in bone metabolism results in the destruction of the bone’s microstructure and an increase in bone brittleness, thereby increasing the risk of fractures. Osteoporosis has complex causes, one of which is related to the dysregulation of 5-hydroxytryptamine, a neurotransmitter closely associated with bone tissue metabolism. Dysregulation of 5-HT directly or indirectly promotes the occurrence and development of osteoporosis. This paper aims to discuss the regulation of 5-HT by Traditional Chinese Medicine and its impact on bone metabolism, as well as the underlying mechanism of action. The results of this study demonstrate that Traditional Chinese Medicine has the ability to regulate 5-HT, thereby modulating bone metabolism and improving bone loss. These findings provide valuable insights for future osteoporosis treatment.
Landscape composition is a stronger determinant than noise and light of avian community structure in an urbanizing county
Authors: Sanchez Karina A. , Benedict Lauryn , Holt Emily A.
Published: October 2023

Urban development has drastically altered ecosystems with dramatic effects on avian diversity and community structure. Most studies investigating this phenomenon are conducted in large and established cities, with fewer conducted in areas of current urbanization. Measuring avian community structure in recently established and growing cities is necessary to understand which species are being affected in a range of urban environments. There is also a critical need to explore multiple measures of urbanization, as different axes of human influence might have varied effects on wildlife. This study investigated the relationship between avian communities and urban noise, light, and landscape composition in Weld County, CO, one of the United States’ fastest growing metropolitan areas. We tested the general hypothesis that avian community assemblages would vary with multiple urban characteristics. We measured noise, light, and landscape composition across 16 sites ranging from urban to non-urban and paired these data with avian species census data. We used multivariate analyses to test for covariance of our three measures of urban characteristics, and to examine patterns of species presence and abundances at sites. Results suggest that developed land and water covary with avian community structure across our study sites. Anthropogenic noise and light did not drive avian community patterns as strongly as percent developed land and water. These three urban characteristics did not covary or form a linear urban to non-urban gradient, suggesting that urbanization can have patchy and unpredictable effects, highlighting the value of measuring urban characteristics separately.
Specific immune responses after BNT162b2 mRNA vaccination and COVID-19 infection
Authors: Arientová Simona , Matúšková Kateřina , Bartoš Oldřich , Holub Michal , Beran Ondřej
Published: October 2023

Although vaccines against COVID-19 are effective tools in preventing severe disease, recent studies have shown enhanced protection after vaccine boosters. The aim of our study was to examine the dynamics and duration of both humoral and cellular immune responses following a three-dose regimen of the BNT162b2 mRNA vaccine. In a longitudinal prospective study we enrolled 86 adults who received the BNT162b2 vaccine, 35 unvaccinated individuals with a history of mild COVID-19 and a control group of 30 healthy SARS-CoV-2 seronegative persons. We assessed the SARS-CoV-2-specific T cell responses and IgG production up to 12 months post the third BNT162b2 dose in 24 subjects. The vaccinated group had significantly higher IgG antibody levels after two doses compared to the convalescent group (p<0.001). After the third dose, IgG levels surged beyond those detected after the second dose (p<0.001). Notably, these elevated IgG levels were maintained 12 months post the third dose. After two doses, specific T cell responses were detected in 87.5% of the vaccinated group. Additionally, there was a significant decrease before the third dose. However, post the third dose, specific T cell responses surged and remained stable up to the 12-month period. Our findings indicate that the BNT162b2 vaccine induces potent and enduring humoral and cellular responses, which are notably enhanced by the third dose and remain persistant without a significant decline a year after the booster. Further research is essential to understand the potential need for subsequent boosters.
Strength development and hydration products of alkali-activated Pisha sandstone geopolymer cement
Authors: Dong Jingliang , Li Lianghua , Shi Zhenhua , Ding Yang , Chen Xiaolei , Zong Yingliang
Published: October 2023

Pisha sandstone (PS) is a unique geological structure in the Yellow River basin in China and is a general term for a rock interlayer composed of sandstone, sand shale, and muddy sandstone. The collapsibility of PS results in a high erosion rate and poor vegetation due to its low diagenetic potential and weak structural strength. This study showed that PS can be converted into geopolymer cement by mixing with a suitable alkali activator. PS was converted to geopolymer cement for construction to control soil erosion and conserve the soil and water in this area. Slag was used as a mineral additive to improve the performance of alkali-active PS geopolymer cement in this study. The influence of slag replacement level, NaOH dosages, and curing age on the compressive strength of alkali-activated PS was investigated. Scanning electron microscopy (SEM)/energy dispersive X-ray (EDS), thermogravimetric analyses (TG/DTG), and X-ray diffraction (XRD) were used to analyze the hydration products and microstructure of alkali-activated PS. The results showed that when the samples had 40 wt.% slag, 1.5 wt.% NaOH, and 4.0 wt.% Na2SiO3, their compressive strength could reach 82.0 MPa at 90 days. Compared with the samples with activator and without activator, the compressive strength increased by 6,664% and 9,011%, respectively. The hydration products were C-S-H gel, geopolymer gel, and calcium carbonate crystals. With 10 wt.% slag as a mineral additive, 1.5 wt.% NaOH, and 4 wt.% Na2SiO3 as an activator, the carbonation ratio of C-S-H gel was 49.3%.
FDEM investigation on the crack propagation characteristics of walnut shell under multi-contact loadings
Authors: Han Bowen , Su Gege , Zeng Yong , Yang Jialong , Fan Xiuwen , Zhang Yongcheng , Zhang Hong , Wang Jianping
Published: October 2023

Walnut shell breaking is an important aspect of post-harvest processing. However, shell-breaking results differ significantly from ideal uniaxial compression results. In this paper, the effect of multipoint loading on the walnut shell-breaking mechanism by combining FDEM with cohesive elements was investigated. The model for walnuts was created using micro-X-ray CT scans and experimental data. Simulation parameters describing mechanical properties were calibrated through modeling the uniaxial compression experiment. The loading point locations were quantitatively described in terms of top and azimuthal angles. The results show that the crack ex-tension rate increases with the number of loading points and the singularity value, and the maximum value of the four-point loading speed is about 450 m/s. The number of cracks is only related to the number of loading points and is not related to the location of the loading point. In addition, three loading methods are proposed, and the fastest crack propagation rate is achieved when the azimuthal angle of four-point loading is 0, 150, 180, and 330, respectively. These findings may serve as a theoretical foundation for studying shell crushing.
Philadelphia chromosome-negative myeloproliferative chronic neoplasms: is clonal hematopoiesis the main determinant of autoimmune and cardio-vascular manifestations?
Authors: Fulvio Giovanni , Baldini Chiara , Mosca Marta , di Paolo Antonello , Bocci Guido , Palumbo Giuseppe Alberto , Cacciola Emma , Migliorini Paola , Cacciola Rossella , Galimberti Sara
Published: October 2023

In this article, we reviewed the possible mechanisms linking the clonal hematopoiesis of indeterminate potential (CHIP) to chronic myeloproliferative neoplasms (MPNs), autoimmune diseases (ADs), and cardiovascular diseases (CADs). CHIP is characterized by the presence of clonal mutations with an allelic frequency >2% in the peripheral blood without dysplasia, overt hematological neoplasms, or abnormalities in blood cell count. The prevalence may reach 20% of elderly healthy individuals and is considered a risk factor for myelodysplastic neoplasms and acute leukemia. In MPNs, CHIP is often associated with mutations such as JAK2V617F or DNMT3A, TET2, or ASXL1, which exhibit a 12.1- and 1.7–2-fold increase in CADs. Specifically, JAK2-mutated cells produce excessive cytokines and reactive oxygen species, leading to proinflammatory modifications in the bone marrow microenvironment. Consequently, the likelihood of experiencing thrombosis is influenced by the variant allele frequency (VAF) of the JAK2V617F mutation, which also appears to be correlated with anti-endothelial cell antibodies that sustain thrombosis. However, DNMT3A mutations induce pro-inflammatory T-cell polarization and activate the inflammasome complex, while TET2 downregulation leads to endothelial cell autophagy and inflammatory factor upregulation. As a result, in patients with TET2 and DNMT3A-related CHIP, the inflammasome hyperactivation represents a potential cause of CADs. CHIP also occurs in patients with large and small vessel vasculitis, while ADs are more frequently associated with MPNs. In these diseases, monocytes and neutrophils play a key role in the formation of neutrophil extracellular trap (NET) as well as anti-endothelial cell antibodies, resulting in a final procoagulant effect. ADs, such as systemic lupus erythematosus, psoriasis, and arthritis, are also characterized by an overexpression of the Rho-associated coiled-coil containing protein kinase 2 (ROCK2), a serine/threonine kinase that can hyperactivate the JAK-STAT pathway. Interestingly, hyperactivation of ROCK2 has also been observed in myeloid malignancies, where it promotes the growth and survival of leukemic cells. In summary, the presence of CHIP, with or without neoplasia, can be associated with autoimmune manifestations and thrombosis. In the presence of these manifestations, it is necessary to consider a “disease-modifying therapy” that may either reduce the clonal burden or inhibit the clonally activated JAK pathway.
A genotype–phenotype correlation in split-hand/foot malformation type 1: further refinement of the phenotypic subregions within the 7q21.3 locus
Authors: Sowińska-Seidler Anna , Socha Magdalena , Szoszkiewicz Anna , Materna-Kiryluk Anna , Jamsheer Aleksander
Published: October 2023

Background: Split-hand/foot malformation type 1 (SHFM1) refers to the group of rare congenital limb disorders defined by the absence or hypoplasia of the central rays of the autopods with or without accompanying anomalies, such as hearing loss, craniofacial malformation, and ectodermal dysplasia. Consequently, the condition is characterized by clinical variability that hinders diagnostic and counseling procedures. SHFM1 is caused by pathogenic variants affecting the DLX5/6 genes and/or their tissue-specific enhancers at the 7q21.3 locus. Herein, we report on seven patients from five unrelated Polish families affected by variable symptoms of the SHFM1 spectrum, all harboring 7q21.3 or 7q21.2-q21.3 rearrangements, and provide a genotype–phenotype correlation in the studied cohort.

Methods: We applied GTG banding, array-based comparative genomic hybridization (aCGH), and whole-genome sequencing (WGS) in order to identify the causative aberrations in all affected patients.

Results: The identified pathogenic structural variants included deletions and/or translocations involving the 7q21.3 locus, i.e., t(7;10)(q21.3;q22.2) and t(7;12)(q21.3;q21.2) in all affected individuals. Interestingly, a sporadic carrier of the latter aberration presented the SHFM1 phenotype with additional features overlapping with Baker–Gordon syndrome (BAGOS), which resulted from the translocation breakpoint at chromosome 12 within the SYT1 gene.

Conclusion: Clinical variability of the studied cohort reflects the composition of the DLX5/6 regulatory elements that were dislocated from their target genes by chromosomal rearrangements. The correlation of our data with the previously published observations enabled us to update the phenotypic subregions and regulatory units within the SHFM1 locus. In addition, we present the first case of SHFM1 and BAGOS-like phenotype that resulted from translocation breakpoints at chromosomes 7 and 12, both of which were pathogenic, and consequently, we show the first evidence that BAGOS can also result from the regulatory loss-of-function SYT1 mutations. In this paper, we emphasize the utility of sequence-based approaches in molecular diagnostics of disorders caused by regulatory structural variants.
Fluid shear stress triggers cholesterol biosynthesis and uptake in inner medullary collecting duct cells, independently of nephrocystin-1 and nephrocystin-4
Authors: Garfa Traoré Meriem , Roccio Federica , Miceli Caterina , Ferri Giulia , Parisot Mélanie , Cagnard Nicolas , Lhomme Marie , Dupont Nicolas , Benmerah Alexandre , Saunier Sophie , Delous Marion
Published: October 2023

Renal epithelial cells are subjected to fluid shear stress of urine flow. Several cellular structures act as mechanosensors–the primary cilium, microvilli and cell adhesion complexes–that directly relay signals to the cytoskeleton to regulate various processes including cell differentiation and renal cell functions. Nephronophthisis (NPH) is an autosomal recessive tubulointerstitial nephropathy leading to end-stage kidney failure before adulthood. NPHP1 and NPHP4 are the major genes which code for proteins that form a complex at the transition zone of the primary cilium, a crucial region required for the maintenance of the ciliary composition integrity. These two proteins also interact with signaling components and proteins associated with the actin cytoskeleton at cell junctions. Due to their specific subcellular localization, we wondered whether NPHP1 and NPHP4 could ensure mechanosensory functions. Using a microfluidic set up, we showed that murine inner medullary collecting ductal cells invalidated for Nphp1 or Nphp4 are more responsive to immediate shear exposure with a fast calcium influx, and upon a prolonged shear condition, an inability to properly regulate cilium length and actin cytoskeleton remodeling. Following a transcriptomic study highlighting shear stress-induced gene expression changes, we showed that prolonged shear triggers both cholesterol biosynthesis pathway and uptake, processes that do not seem to involve neither NPHP1 nor NPHP4. To conclude, our study allowed us to determine a moderate role of NPHP1 and NPHP4 in flow sensation, and to highlight a new signaling pathway induced by shear stress, the cholesterol biosynthesis and uptake pathways, which would allow cells to cope with mechanical stress by strengthening their plasma membrane through the supply of cholesterol.
The surgical strategy and technical nuances of in situ side-to-side bypass for the management of complex intracranial aneurysms
Authors: Wang Hua-wei , Xue Zhe , Sun Cai-hong , Kong Dong-sheng , Wu Chen , Sun Zheng-hui
Published: October 2023

Background

Despite continuous advances in microsurgical and endovascular techniques, the treatment of complex aneurysms remains challenging. Aneurysms that are dilemmatic for conventional clipping or endovascular coiling often require bypass as part of a strategy to reduce the risk of ischemic complications. In anatomically favorable sites, the intracranial–intracranial in situ bypass may be an appealing choice. This article details the surgical strategies, operative nuances, and clinical outcomes of this technique with a consecutive series in our department.

Methods

A retrospective review of a prospectively maintained neurosurgical patient database was performed to identify all patients treated with side-to-side in situ bypass from January 2016 to June 2022. In total, 12 consecutive patients, including 12 aneurysms, were identified and included in the series. The medical records, surgical videos, neuroimaging studies, and follow-up clinic notes were reviewed for every patient.

Results

Of the 12 aneurysms, there were 5 middle cerebral artery aneurysms, 4 anterior cerebral artery aneurysms, and 3 posterior inferior cerebellar artery aneurysms. The morphology of the aneurysms was fusiform in 8 patients and saccular in the remaining 4 patients. There were 3 patients presented with subarachnoid hemorrhage. The treatment modality was simple in situ bypass in 8 cases and in situ bypass combined with other modalities in 4 cases. Bypass patency was confirmed in all cases by intraoperative micro-doppler probe and (or) infrared indocyanine green (ICG) video angiography intraoperatively and with digital subtraction angiography (DSA) or computed tomography angiography (CTA) postoperatively. None of the patients developed a clinically manifested stroke due to the procedure though a callosomarginal artery was intentionally removed in one patient. The median follow-up period was 16.2 months (6-36). All patients had achieved improved or unchanged modified Rankin scale scores at the final follow-ups.

Conclusion

Cerebral revascularization technique remains an essential skill for the treatment of complex aneurysms. The in situ bypass is one of the most effective techniques to revascularize efferent territory when vital artery sacrifice or occlusion is unavoidable. The configuration of in situ bypass should be carefully tailored to each case, with consideration of variations in anatomy and pathology of the complex aneurysms.
Quantitative EEG analysis in typical absence seizures: unveiling spectral dynamics and entropy patterns
Authors: Guerrero-Aranda Alioth , Ramírez-Ponce Evelin , Ramos-Quezada Oscar , Paredes Omar , Guzmán-Quezada Erick , Genel-Espinoza Alejandra , Romo-Vazquez Rebeca , Vélez-Pérez Hugo
Published: October 2023

A typical absence seizure is a generalized epileptic event characterized by a sudden, brief alteration of consciousness that serves as a hallmark for various generalized epilepsy syndromes. Distinguishing between similar interictal and ictal electroencephalographic (EEG) epileptiform patterns poses a challenge. However, quantitative EEG, particularly spectral analysis focused on EEG rhythms, shows potential for differentiation. This study was designed to investigate discernible differences in EEG spectral dynamics and entropy patterns during the pre-ictal and post-ictal periods compared to the interictal state. We analyzed 20 EEG ictal patterns from 11 patients with confirmed typical absence seizures, and assessed recordings made during the pre-ictal, post-ictal, and interictal intervals. Power spectral density (PSD) was used for the quantitative analysis that focused on the delta, theta, alpha, and beta bands. In addition, we measured EEG signal regularity using approximate (ApEn) and multi-scale sample entropy (MSE). Findings demonstrate a significant increase in delta and theta power in the pre-ictal and post-ictal intervals compared to the interictal interval, especially in the posterior brain region. We also observed a notable decrease in entropy in the pre-ictal and post-ictal intervals, with a more pronounced effect in anterior brain regions. These results provide valuable information that can potentially aid in differentiating epileptiform patterns in typical absence seizures. The implications of our findings are promising for precision medicine approaches to epilepsy diagnoses and patient management. In conclusion, our quantitative analysis of EEG data suggests that PSD and entropy measures hold promise as potential biomarkers for distinguishing ictal from interictal epileptiform patterns in patients with confirmed or suspected typical absence seizures.
Integrated widely targeted metabolomics and network pharmacology revealed quality disparities between Guizhou and conventional producing areas of Codonopsis Radix
Authors: Zhang Kaixian , Zhang Delin , Yang Qingfang , Long Langtao , Xie Jian , Wang Yong , Yao Qiuyang , Wu Faming , Liu Sha
Published: October 2023

Introduction

With the internationalization of traditional Chinese medicine, the demand for medicinal and edible Codonopsis Radix (CR) has increased, and its medicinal resources have attracted attention. CR is a well-known traditional Chinese medicine with a long pharmaceutical and edible history. The Guizhou province in China has abundant CR resources, but in the absence of systematic studies on species identification and chemical compositions, the capacity of the capacity of the province to CR resource has not been fully utilized.

Methodology

We used plant morphology and DNA barcoding techniques to identify Luodang (LD) and Weidang (WD) species. To investigate the differences in metabolites between LD and WD, as well as three Chinese Pharmacopeia CRs, and to predict pharmacological mechanisms of action for the dominant differential metabolites, we utilized widely targeted metabolomics and network pharmacology. The results also revealed the material basis for the excellent food properties of both LD and WD.

Results

The plant traits and DNA barcoding molecular identification results indicated that Luodang and Weidang from Guizhou were Codonopsis tangshen and Codonopsis pilosula, respectively. Widely targeted metabolomics analysis revealed that a total of 1,116 metabolites from 14 categories, including phenolic acids, lipids, flavonoids, were found in five CRs and shared 1,054 (94.4%) metabolites. LD and WD each contained 3 and 10 dominant differential metabolites, respectively, which were primarily flavonoids and amino acids. Amino acids, phenolic acids, and organic acids play important roles in their excellent food attributes. In CR, eight dominant differential metabolites were discovered for the first time, including isoorientin-7-O-(6″-feruloyl) glucoside, N-formyl-L-methionine, and cyclo (Phe-Glu), among others. Network pharmacology analyses showed that, in LD, dominant differential metabolites were closely related to anti-tumor, cardiovascular disease improvement, nervous system protection, and metabolic disease treatment, whereas in WD, they were closely related to nervous system protection and cardiovascular disease improvement.

Conclusion

The species of LD and WD were included in the Chinese Pharmacopeia, and their metabolite profiles were remarkably similar to CR from traditional producing areas. Therefore, LD and WD can be used and promoted medicinally as CR, and they have potential value for new drug development. This study enriched the database of CR compounds and provided a reference for quality control, resource development, and new drug development of CR.
Case Report: Termination of unplanned pregnancy led to rapid deterioration of non-small-cell lung cancer during osimertinib treatment
Authors: Ma Qizhi , Shu Pei , Zhou Kexun , Wang Yongsheng
Published: October 2023

We present a case of a woman with non-small-cell lung cancer (NSCLC) who experienced disease progression during treatment with the epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) osimertinib due to an unplanned pregnancy. Given the risk of tumor progression, the patient underwent an artificial abortion. However, disease deterioration occurred shortly after termination of the pregnancy, with severe chest pain, increased dyspnea, and pleural effusion. After positive rescue measures, including emergency thoracic drainage, thoracentesis, and oxygen uptake, her symptoms improved. Considering pregnancy as an immune escape physiological process, the patient continued treatment with osimertinib, and a partial response (PR) lasting 16 months was observed. Therefore, this case highlights the importance of being vigilant about the rapid development of the tumor after delivery in pregnant patients with EGFR-mutation lung cancer and taking preventive measures to cope with various emergencies.