bluuebunny/crossref_metadata_2025 huggingface dataset:
Single oblique osteotomy for correction of antebrachial angular and torsional deformities in a dog
Authors: Kim Sun-Young , Snowdon Kyle A. , DeCamp Charles E.
Published: July 2017

CASE DESCRIPTION A 9-month-old 4.6-kg (10.1-lb) spayed female Shih Tzu was examined for a 4-week history of left forelimb lameness.

CLINICAL FINDINGS Moderate left forelimb lameness was present when weight bearing, accompanied by buckling of the left carpal joint and moderate signs of pain with hyperextension of the elbow joint. A 32° angular deformity of the left radius in a 45° craniomedial oblique plane (equivalent to 23° valgus and 23° procurvatum) and a 55° external torsional deformity were measured on 3-D reconstructed CT images. Humeroulnar incongruity of the left elbow joint was also evident. Single oblique osteotomy (SOO) and dynamic proximal ulnar osteotomy were recommended.

TREATMENT AND OUTCOME Creation of a 3-D printed bone model from reconstructed CT images aided surgical planning. The SOO was located 45° medial to the sagittal plane of the left radius at the level of the center of rotation of angulation. The SOO was oriented 32° distolaterally from the transverse plane of the radius and traversed the left ulna. The bones were rotated along the osteotomy into grossly proper alignment and stabilized with a plate and bone screws. Dynamic proximal ulnar osteotomy was then performed. Six months after surgery, radiographs showed remodeling at the SOO site and the lameness had resolved. The owners expressed satisfaction with the outcome.

CLINICAL RELEVANCE In dogs with angular and torsional long bone deformities, SOO may be a viable alternative to wedge osteotomies. The SOO simultaneously addressed angular and torsional deformities without bone loss and provided rigid internal fixation.
Surgical treatment of a paraspinal abscess with osteomyelitis and spinal cord compression in a rabbit
Authors: Crawford Abbe H. , Hedley Joanna E. , Lam Richard , Drożdżyńska Maja J. , De Decker Steven
Published: July 2017

CASE DESCRIPTION A 16-month-old neutered male Continental Giant rabbit (Lepus curpaeums) was referred for evaluation of a 7-day history of acute-onset, progressive, symmetric paraparesis.

CLINICAL FINDINGS On initial examination, the rabbit was nonambulatory, and results of neurologic examination were consistent with a lesion affecting the T3-L3 spinal cord segments. Thoracic radiography showed irregular widening of the left T11–12 articular process joint. Marked dorsolateral and lateral extradural spinal cord compression with contrast enhancement of the adjacent epaxial muscles was evident on MRI images of the spine.

TREATMENT AND OUTCOME A left-sided T11–T12 hemilaminectomy was performed, which revealed an abnormal and hypertrophic T11–12 articular process joint and an osteolytic lesion communicating with the vertebral canal. Copious purulent material causing marked spinal cord compression was evident, and the surgical site was lavaged extensively with sterile (0.9% NaCl) saline solution. Results of aerobic, anaerobic, and enriched bacteriologic cultures of swab specimens obtained from the surgical site were negative. Histologic analysis of biopsy samples revealed chronic purulent osteomyelitis, myositis, and fasciitis with necrosis, fibrosis, and dystrophic mineralization. The rabbit was discharged 48 hours after surgery. Ten weeks after surgery, the rabbit was ambulatory with mild paraparesis. On telephone follow-up 21 months after surgery, the owners indicated that the rabbit was healthy and expressed satisfaction with the treatment and outcome.

CLINICAL RELEVANCE Paraspinal abscess with vertebral canal involvement should be considered as a differential diagnosis for rabbits with clinical signs of progressive T3-L3 myelopathy. Outcome for the patient of the present report suggested that surgical treatment including decompression and debridement can result in a favorable long-term outcome.
Randomized blinded controlled trial to assess the association between a commercial vaccine against Moraxella bovis and the cumulative incidence of infectious bovine keratoconjunctivitis in beef calves
Authors: Cullen Jonah N. , Engelken Terry J. , Cooper Vickie , O'Connor Annette M.
Published: July 2017

OBJECTIVE To assess the association between a commercially available vaccine against Moraxella bovis and cumulative incidence of infectious bovine keratoconjunctivitis (IBK) from processing to weaning (primary objective) and body weight at weaning (secondary objective).

DESIGN Randomized blinded controlled trial.

ANIMALS 214 calves (≥ 2 months of age) born in the spring of 2015 at an Iowa State University cow-calf research unit with no visible lesions or scars on either eye.

PROCEDURES Calves were randomly allocated to receive SC administration of a single dose of a commercial vaccine against M bovis (112 enrolled and 110 analyzed) or saline (0.9% NaCl) solution (111 enrolled and 104 analyzed). Calves were monitored for signs of IBK from treatment to weaning, and body weight at weaning was recorded. People involved in calf enrollment and outcome assessment were blinded to treatment group assignment. Cumulative incidence of IBK and weaning weight were compared between vaccinated and unvaccinated calves; the effect measure was the risk ratio and mean difference, respectively.

RESULTS IBK was detected in 65 (59.1%) vaccinated calves and 62 (59.6%) unvaccinated calves (unadjusted risk ratio, 0.99; 95% confidence interval, 0.79 to 1.24) during the study period. No significant difference in weaning weights was identified between vaccinated and unvaccinated calves (unadjusted effect size, 4.40 kg [9.68 lb]; 95% confidence interval, −3.46 to 12.25 kg [−7.61 to 26.95 lb]).

CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that the commercially available M bovis vaccine was not effective in reducing the cumulative incidence of IBK or increasing weaning weight in beef calves.
Innovación y competitividad del sistema lechero en Valles Centrales de Querétaro. Hacia un modelo de gobernanza territorial
Authors: Delgadillo Macías Javier , Montaño Becerril Elizabeth
Published: July 2017

Los objetivos del trabajo consisten en analizar las formas de organización productiva del sistema de lácteos de la región Valles Centrales en Querétaro y reconocer las distintas modalidades de gobernanza que se han construido a partir de la interacción de agentes productivos y de sus incidencias organizativas territoriales. La metodología parte del enfoque milieux innovateurs que permite documentar los efectos del proceso de cambio tecnológico del sistema lácteo respecto del sistema lechero y analizar la competitividad territorial mediante la evaluación de los impactos en el nivel de eficiencia con el cual las empresas combinan sus recursos humanos, tecnológicos y de capital en el ámbito regional. El cuestionamiento general que rige la investigación es: ¿Qué concesiones (ventajas, privilegios, beneficios) han construido los actores de este territorio para desplegar procesos de cambio tecnológico efectivos en la competitividad del sistema lácteo regional? Como resultado se encontró que la principal consecuencia de las interacciones locales en torno a la lechería en Querétaro responde a las estrategias del nivel global más que a una visión de desarrollo regional, no obstante poseer este territorio vocación cultural, natural y manifestarse como un medio innovador. La limitación principal se debió a la insuficiente información sobre la organización interna de las grandes empresas del conglomerado estudiado. Las conclusiones señalan cómo el aprovechamiento disímil de los recursos naturales y del potencial humano han redefinido los patrones de especialización lechera y han coadyuvado a concentrar la renta del suelo en un grupo selecto de actores del territorio en estudio.
Globalización y nostalgia. Cambios en la alimentación de familias yucatecas
Authors: Ayuso Peraza Giselle , Castillo León María Teresita
Published: July 2017

El objetivo es contribuir a la comprensión del modo en el que familias de la ciudad de Mérida, Yucatán, México, han percibido la evolución de sus modos de alimentación a partir de la filtración de la globalización en su vida cotidiana. Metodología. Participaron nueve personas de distintos tipos de familias y estratos socioeconómicos para indagar los significados que dan a la alimentación y la comida, mediante una metodología cualitativa, realizando entrevistas abiertas y observaciones directas. Resultados. Expresan la forma en la que los meridanos han integrado los alimentos procesados exponiendo los factores socioeconómicos que perciben como los detonantes de esos cambios. Limitaciones. Se describen las consecuencias de carácter psicosocial que estos cambios han traído las cuales se centran, en primera instancia, en la incertidumbre sobre lo que es sano consumir y, por otro lado, la nostalgia por aquello que se consumía con anterioridad. Conclusiones. Se puede argumentar que, en materia de alimentación, las familias han tenido que adaptarse de manera creativa, para sobrevivir en el mundo globalizado y que, de cierta manera, se ha dado un constante alejamiento de las tradiciones culinarias, debido principalmente a aspectos socioeconómicos. De igual modo, el discurso social de lo que es sano ha modificado parcialmente las dietas tradicionalmente yucatecas para comenzar a adaptarse a un patrón homogeneizado de lo saludable.
Hipoacusia neurosensorial en una empresa del sector madera
Authors: Calderón Darío , Bedoya Elias , Ramírez Nancy
Published: July 2017

La hipoacusia neurosensorial inducida por ruido es la disminución de la capacidad auditiva por alteración a nivel del oído interno,del octavo par craneal o de las vías auditivas centrales. Este tipo dealteraciones comprometen y modifican finalmente la sensibilidad coclear. Dichapérdida auditiva se produce a lo largo del tiempo como consecuencia de una exposición crónica a ruidos superiores a 85 decibeles. Aunque la susceptibilidad a la pérdida de audición inducida por el ruido varía considerablemente de una persona a otra, casi todo el mundo pierde algo de audición si está expuesto durante el tiempo adecuado a un ruido suficientemente intenso.
La enseñanza y el aprendizaje de la energía y sus transformaciones con una simulación
Authors: Pandiella Susana Beatriz , Díaz Leticia Beatriz
Published: July 2017

El objetivo de esta comunicación es relatar la implementación de una secuencia de enseñanza que utiliza un software abierto (simulación) para facilitar el aprendizaje de la energía y sus transformaciones, así como también de las competencias y habilidades científicas relacionadas con dichos conceptos, por parte de estudiantes de una escuela secundaria. Del análisis de los resultados puede inferirse que la simulación utilizada constituyó un valioso asistente para la enseñanza y el aprendizaje de estos conceptos y habilidades científicas. La simulación actuó como mediadora entre el modelo físico y el estudiante para construir conceptos y procedimientos aceptados por la Ciencia, con la guía y la orientación del profesor.
¿Están funcionando los programas de recolección selectiva de residuos post-consumo? Caso: percepción en Bogotá
Authors: Gómez Méndez Luis David
Published: July 2017

Mediante encuestas, se evaluó la percepción que tienen los usuarios de productos post-consumo de medicamentos vencidos, computadores, bombillas, pilas y llantas en la ciudad de Bogotá como parte de los Planes de Recolección Selectiva y Gestión Ambiental de residuos que viene implementando el Ministerio de Medio Ambiente y Desarrollo Sostenible (antes Ministerio de Ambiente, Vivienda y Desarrollo Territorial) desde el año 2009 con el apoyo de la Asociación Nacional de Empresarios de Colombia (ANDI). Se evidencia un desconocimiento por parte de los consumidores de estos planes de recolección debido a la falta de información que existen entre los diferentes actores involucrados (productores, distribuidores y comercializadores) acerca del ciclo de vida de los estos productos.
Editorial
Authors: Teknos Revista
Published: July 2017

La facultad de Ingeniería de la Fundación Universidad Tecnológico Comfenalco tienen el gusto de presentarles la edición 2015 – 1 de la revista TEKNOS Ingeniería. En esta oportunidad presentamos a la comunidad académica un conjunto interesante de artículos de diversas campos de la ingeniería.
Medición de habilidades de trabajo en equipo en un modelo distribuido y colaborativo
Authors: Aballay Laura N. , Herrera Myriam B. , Collazos César A , Aciar Silvana V.
Published: July 2017

Se exponen los resultados, respecto a habilidades sociales y de trabajo en equipo de los alumnos, luego de una primera aplicación de un modelo enseñanza-aprendizaje colaborativo y distribuido propuesto. Se llevó a cabo un experimento controlado, el cual tuvo lugar en ambiente universitario y cuyos sujetos fueron estudiantes avanzados de los programas de grado en Informática pertenecientes a cuatro universidades públicas de Latinoamérica. La aplicación del modelo facilitó el trabajo colaborativo entre las instituciones educativas geográficamente dispersas y, además de brindar una metodología para la enseñanza y el aprendizaje de tópicos relacionados con la evaluación de la Usabilidad de Interfaces, se recopiló información referida al desarrollo de habilidades de trabajo en equipo por parte de los alumnos participantes de dicha experiencia. Para medir el impacto del modelo en las denominadas «habilidades blandas» de los alumnos, se utilizó como herramienta un cuestionario.
Proyecto ambiental escolar (PRAE) para la recuperación de suelos en la Institución Educativa Técnica de Salamanca, Samacá –Boyacá
Authors: Pita Morales Luz Adriana , García Judy , Collazos César A , Monroy Verónica , Ruano Carlos , Castiblanco Juan Sebastián
Published: July 2017

El municipio de Samacá ubicado en el departamento de Boyacá, posee un gran patrimonio natural de paramos y riqueza hídrica. Debido a su ubicación y topografía cuenta con una gran oferta en recursos forestales y de biodiversidad que son sustento del desarrollo regional y de la multiplicidad del uso del territorio (Plan de Desarrollo Municipal, 2012), sin embargo la cobertura del suelo se ha disminuido debido a la acción antrópica sobre los recursos naturales con actividades intensivas de deforestación, explotación minera, extensión de la frontera agrícola, contaminación de humedales y ríos con residuos sólidos, urbanización, sumado a esto se observa la despreocupación de los habitantes del municipio por el ambiente. En este sentido la minería es la actividad económica más relevante de la región con explotaciones de carbón en su mayoría a cielo abierto las cuales han dejado deteriorados muchos suelos y produce una acelerada contaminación del aire por material particulado, la vereda de Salamanca es una de las más afectadas por estos fenómenos, en especial la Institución Educativa Técnica de Salamanca, que además está ubicada en medio de dos caminos rurales en construcción muy transitados, este colegio exhibe una problemática aguda en contaminación del aire que ha generado enfermedades pulmonares a los estudiantes y profesores. En este marco, se plantea un proyecto de recuperación de suelos encaminado en un principio al establecimiento de una cerca viva de árboles, que sirven de barrera para aislar el material particulado proveniente del arreglo de carreteras y de la explotación minera, y en segundo lugar se plantea la construcción de un vivero de plantas nativas de la región con el fin de que el colegio se convierta en epicentro de la recuperación y restauración de los suelos deteriorados por la minería. El colegio Instituto Técnico de Salamanca, a través del proyecto ambiental escolar –PRAE se ha sensibilizado y capacitado en el cuidado del ambiente, los recursos naturales, suelo, agua, flora fauna, manejo de residuos sólidos y técnicas de reforestación, a través del montaje y construcción del vivero, además de incluir como prioridad la educación ambiental con el fin de fomentar en la región el cuidado de la naturaleza.
Exploración de técnicas analíticas para ensayos de crudo desarrollados en laboratorios de Ecopetrol, Saybolt y Universidad de Cartagena
Authors: Mendoza Beltrán Dina
Published: July 2017

El objetivo de este estudio fue explorar en tres pasos técnicas analíticas para ensayos en crudo que se desarrollan en tres laboratorios, Ecopetrol, Saybolt y Universidad de Cartagena, frente a las técnicas exigidas internacionalmente por la norma ASTM. Primero, se revisaron normas ASTM y aquellas utilizadas por Instituto Colombiano de Petróleo (ICP), se listó el número de ensayos necesarios para la caracterización de crudos. En segundo lugar, se indagaron los ensayos que se realizan en tres laboratorios de Cartagena (Colombia), se revisó la norma utilizada y el cumplimiento en equipamiento frente a estándares. Tercero: se buscó cuáles ensayos estaban acreditados bajo norma ISO/IEC17025. Al final, se determinó que de los 24 ensayos que se establecen en norma ASTM para caracterización de crudos, los laboratorios de Ecopetrol y Universidad de Cartagena realizan 19 y 11 respectivamente; por su parte, Saybolt, 13. De los ensayos que se realizan en ICP en ASSAY tipo 1, Ecopetrol lleva a cabo 11; Universidad de Cartagena, 9 y Saybolt, 8 ensayos. Al verificar los equipos utilizados, se obtuvo que Ecopetrol utiliza el 94,73 %; Saybolt, 84,6 1% y Universidad de Cartagena, 87,5 % de los equipos que estipula la norma ASTM; además, el laboratorio de Ecopetrol tiene acreditado el 74 % de sus ensayos.
Software libre para apoyo a los procesos educativos
Authors: Duque Méndez Néstor Darío , Uribe Hurtado Ana Lorena , Tabares Morales Valentina
Published: July 2017

Este artículo se orienta a mostrar el software libre como un camino relevante en los procesos educativos. Se muestran diferentes dimensiones que soportan esta afirmación, que van desde la filosofía del software libre, pasando por lo relacionado con los costos asociados y terminando con las alternativas en casi todos los campos del conocimiento. Se da especial importancia al beneficio ofrecido de potenciar competencias y valores que, casi siempre, en el software propietario, estarían restringidas; en particular, la posibilidad abierta para desarrollar a partir de trabajos previos de la comunidad, fortaleciendo capacidades tecnológicas y la creatividad y promoviendo la construcción colectiva altruista sin protagonismo. Finalmente, se presenta una propuesta para un Laboratorio Académico Genérico basado en Software Libre, cuyo aporte fundamental es mostrar que existen los elementos tecnológicos que permiten implementar, en diferentes ambientes, plataformas educativas completas para diferentes tipos de usuario.
Jogo educativo ambiental – O desafio de Gaia
Authors: Kleinkauf Paola , Ziza Magni Ederson G. , Rosecler Bez Marta
Published: July 2017

Este artigo apresenta o experimento realizado com o ambiente de jogo Desafio de Gaia para uso em aulas de Educação Ambiental. O ambiente é formado por quatro temáticas: Água, Energia, Resíduos e Mobilidade. O jogo foi aplicado em uma turma de sexto ano de uma escola municipal e contou com a participação de 12 alunos. Foi realizado um pré-teste sobre o tema educação ambiental, antes do uso do jogo, seguido de um pós-teste. Os resultados apresentam evidências de aprendizado do tema tão somente com o uso do ambiente. Após o experimento, os alunos responderam a um teste de percepção sobre o uso do ambiente de jogo.
Editorial
Authors: Teknos Revista
Published: July 2017

O presente número da Revista da Faculdade de Engenharia da Fundación Universitaria Tecnológico Comfenalco (TEKNOS) apresenta artigos selecionados na conferência CAVA 2016 (VIII International Conference of Adaptive and Accessible Virtual Learning Environment.). A conferência é um importante fórum latino-americano no qual cientistas, profissionais e estudantes trocam ideias e compartilham seus resultados de pesquisa no domínio da Aprendizagem Inclusiva e Adaptável. Esse aborda questões relacionadas ao impacto que as tecnologias de informação e comunicação têm tido como sistemas de conexão em processos de treinamento baseados em competências e o impacto dos processos de inclusão para pessoas com necessidades especiais que entram no sistema educacional. Ainda a conferência enfatiza o uso de Ambientes Virtuais de Aprendizagem acessíveis e adaptativos como um mecanismo para aumentar a eficácia dos processos de treinamento, tanto nas empresas quanto nas instituições de Ensino Superior.
Sistema de recomendación de objetos de aprendizaje a través de filtrado colaborativo
Authors: Rodríguez Marín Paula Andrea , Pérez Zapata Ángela María , Londoño Rojas Luis Felipe , Duque Mendez Néstor Darío
Published: July 2017

El uso de plataformas colaborativas en educación se ha incrementado en los últimos años. La gran cantidad de usuarios registrados en las mismas ha originado el problema de no saber con quién interactuar. Muchas veces la persona con quien se interactúa no es la idónea y resultan interacciones no exitosas. En este artículo, se presenta un sistema recomendado que sugiere personas idóneas para interactuar en una plataformacolaborativa.
Task-Specific Training for Adults With Chronic Knee Pain: A Case Series
Authors: Bove Allyn M. , Baker Nancy , Livengood Heather , King Visnja , Mancino Joseph , Popchak Adam , Fitzgerald G. Kelley
Published: July 2017

Study Design

Case series.

Background

Recent evidence suggests that traditional impairment-based rehabilitation approaches for patients with knee pain may not result in improved function or reduced disability. This case series describes a novel task-specific training approach to exercise therapy for patients with chronic knee pain and reports changes in measures of clinical outcome (pain and physical function) following participation in the training program.

Case Description

Seven patients with chronic knee pain aged 40 years or older were included. Each reported at least “moderate” difficulty with sit-to-stand transfers, floor transfers, and/or stair negotiation at baseline. Experienced physical therapists provided between 8 and 16 treatment sessions focusing on improving performance of difficult or painful tasks.

Outcomes

A majority of patients demonstrated clinically important improvements in both patient-rated outcomes (Knee injury and Osteoarthritis Outcome Score, numeric pain-rating scale, modified Arthritis Self-Efficacy Scale) and performance-based outcomes (30-second chair-rise test, timed stair-climb test, floor transfer test, Performance Assessment of Self-Care Skills).

Discussion

A task-specific training approach for patients with chronic knee pain was described and yielded considerable improvement in pain and function for most of the individuals in this case series. Larger studies are needed to determine how task-specific training compares with more traditional impairment-based exercise approaches for chronic knee pain.

Level of Evidence

Therapy, level 5. J Orthop Sports Phys Ther 2017;47(8):548–556. doi:10.2519/jospt.2017.7349
Insight into the pathomorphology of the distal border of the equine navicular bone
Authors: Frątczak Martyna , Włodarek Jan , Frąckowiak Hieronim , Komosa Marcin
Published: July 2017

Pathological changes of the equine navicular bone are found in a variety of forms in numerous old and also relatively young horses. Therefore, investigations on this small bone are of major practical significance. The current article presents a view of the alterations observed on the distal aspect of the bone and analyzes their origin and importance. As a result of pressure subjected by the deep digital flexor tendon and adaptive remodelling of the bone tissue, distal border of the navicular bone extends and becomes sharpened. Osseous distal border fragments may be a consequence of this phenomenon. However, the origin of fragments is also associated with the development of entheseophytes on the margins, which can result from stress induced by the distal impar sesamoid ligament. The authors are not unanimous about the clinical importance of fragments, but it seems that the most dangerous are large-sized ones, especially when occurring together with other lesions. An important issue is also the alterations of the nutrient foramina located on the distal border, due to the main role of this surface in blood supply. Changes in the size and shape of pathological synovial invaginations can be a sign of circulatory disturbances and abnormal turnover of the bone tissue. Some researchers link synovial invaginations to cases of lameness, but their importance is still enigmatic. Majority of studies focus on warmblood horses, and a lower interest in coldblood horses can be seen. Nonetheless, certain data are a strong argument that the breed and morphotype can affect the frequency of adverse changes.
Biochemical and haematological profile of donkeys in the Czech and Slovak Republics: influence of age and sex
Authors: Sedlinská Markéta , Horáčková Eliška , Vyvial Michal , Mráčková Miroslava , Jánová Eva
Published: July 2017

The aim of this study was to evaluate the importance of taking into account the age and sex of the donkeys while assessing individual haematological and biochemical variables. Material used for the study were blood samples collected from 112 clinically healthy donkeys (37 males and 75 females) aged from 05 to 30 years old. Selected haematological and biochemical variables were examined and the influence of sex and age was analyzed by multivariate analysis. Significant differences were found in some of biochemical and haematological variables between groups. The results of this study can be useful for appropriate clinical interpretation of laboratory results in donkeys.
Detection of selected antibiotic resistance genes using multiplex PCR assay in mastitis pathogens in the Czech Republic
Authors: Pyatov Vladimir , Vrtková Irena , Knoll Aleš
Published: July 2017

The aim of this research was to develop multiplex polymerase chain reaction assays for the detection of aminoglycoside (strA, strB), sulphonamide (sulI, sulII), tetracycline (tetA, tetB, tetK, tetM, tetO), macrolide and lincosamide (msrA, ermA, ermB, ermC, mefA/E) genes of resistance in mastitis pathogens (Escherichia coli, Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae and Streptococcus dysgalactiae). Applying the established assays, we investigated the distribution of antibiotic resistance genes in the above mentioned species isolated from milk samples in the Czech Republic. Each assay consisted of seven pairs of primers. Six of them amplified fragments of antibiotic resistance genes and one pair a fragment of a species specific gene. Polymerase chain reaction conditions were optimized to amplify seven gene fragments simultaneously in one reaction. In total, 249 isolates were used, among which 111 were positive for E. coli, 52 for S. aureus and 86 for Streptococcus spp. The majority (60.2%) of bacteria carried at least one antibiotic resistance gene and 44.6% were multidrug-resistant. The designed multiplex polymerase chain reaction assays may be applied as diagnostic method to replace or complement standard techniques of antibiotic susceptibility testing in the mentioned pathogens.
Registration of ‘Sunshine’ Hard White Winter Wheat
Authors: Haley Scott D. , Johnson Jerry J. , Peairs Frank B. , Stromberger John A. , Hudson-Arns Emily E. , Seifert Scott A. , Anderson Victoria A. , Bai Guihua , Chen Xianming , Bowden Robert L. , Jin Yue , Kolmer James A. , Chen Ming-Shun , Seabourn Bradford W.
Published: July 2017

‘Sunshine’ (Reg. No. CV‐1129, PI 674741) hard white winter wheat (Triticum aestivum L.) was developed by the Colorado Agricultural Experiment Station and released in August 2014 through a marketing agreement with the Colorado Wheat Research Foundation. In addition to researchers at Colorado State University, USDA‐ARS researchers at Manhattan, KS, St. Paul, MN, and Pullman, WA participated in its development. Sunshine was developed with the objective of making available a hard white winter wheat cultivar with improved grain yield and straw strength and similar milling and baking qualities to ‘Snowmass’ hard white winter wheat. Both Snowmass and Sunshine are grown in Colorado and adjacent areas of the west‐central Great Plains in an identity‐preserved marketing system whereby a grower premium is paid through a contract with a flour milling company. Sunshine was developed with a modified‐bulk breeding method from the cross KS01HW152‐6/HV9W02‐267W made in 2005 at Fort Collins, CO. Following two generations (F2 and F3) of bulk‐population evaluation, Sunshine was selected as an F3:4 line in July 2009, assigned experimental line number CO09W293, and evaluated in replicated yield trials from 2012 to 2014 in eastern Colorado.
Numerical Study on the Internal Flow Field of a Reversible Turbine during Continuous Guide Vane Closing
Authors: Mao Xiuli , Dal Monte Andrea , Benini Ernesto , Zheng Yuan
Published: July 2017

The unsteady flow field in a reversible pump-turbine is investigated during the continuous load rejection using a 3D computational fluid dynamic analysis. Numerical calculations are carried out using the detached eddy simulation (DES) turbulence model and a new approach involving automatic mesh motion. In this way, the instability of the flow field is analyzed by continuously changing the guide vane openings from the best efficiency point (BEP). Unsteady flow characteristics are described by post-processing signals for several monitoring points including mass flow, torque, head and pressure in the frequency and time-frequency domains. The formation of vortices of different scales is observed from the origin to further enlargement and stabilization; the effect of the rotating structures on the flow passage is analyzed, and the influence of unsteady flow development on the performance of the turbine is investigated. Finally, the evolution during the period of load rejection is characterized in order to determine the hydrodynamic conditions causing the vibrations in the machine.
Exploration of Dynamic Elastic Modulus Changes on Glioblastoma Cell Populations with Aberrant EGFR Expression as a Potential Therapeutic Intervention Using a Tunable Hyaluronic Acid Hydrogel Platform
Authors: Sivakumar Hemamylammal , Strowd Roy , Skardal Aleksander
Published: July 2017

Glioblastoma (GBM) is one of most aggressive forms of brain cancer, with a median survival time of 14.6 months following diagnosis. This low survival rate could in part be attributed to the lack of model systems of this type of cancer that faithfully recapitulate the tumor architecture and microenvironment seen in vivo in humans. Therapeutic studies would provide results that could be translated to the clinic efficiently. Here, we assess the role of the tumor microenvironment physical parameters on the tumor, and its potential use as a biomarker using a hyaluronic acid hydrogel system capable of elastic modulus tuning and dynamic elastic moduli changes. Experiments were conducted to assess the sensitivity of glioblastoma cell populations with different mutations to varying elastic moduli. Cells with aberrant epithelial growth factor receptor (EGFR) expression have a predilection for a stiffer environment, sensing these parameters through focal adhesion kinase (FAK). Importantly, the inhibition of FAK or EGFR generally resulted in reversed elastic modulus preference. Lastly, we explore the concept of therapeutically targeting the elastic modulus and dynamically reducing it via chemical or enzymatic degradation, both showing the capability to reduce or stunt proliferation rates of these GBM populations.
Growth and Tissue Elemental Composition Response of Butterhead Lettuce (Lactuca sativa, cv. Flandria) to Hydroponic Conditions at Different pH and Alkalinity
Authors: Anderson Tyler , Martini Miguel , De Villiers David , Timmons Michael
Published: July 2017

Biomass and tissue elemental differences were quantified for lettuce grown in deep-water conventional hydroponic conditions at two pH and alkalinity conditions. Nutrient solutions were created using inorganic salts and either reverse osmosis (RO) water or municipal water with high alkalinity. Three treatments were evaluated: (a) nutrient solution created with reverse osmosis (RO) water and maintained at pH 5.8 (H5); (b) same as H5 but maintained at pH 7.0 (H7); and (c) nutrient solution created using municipal water and maintained at pH 7.0, referred to as HA7. Averaged across three trials, the HA7 and H7 treatments produced 26% less shoot fresh weight (FW) than the H5 treatment with an 18% reduction in dry weight (DW). The H5 treatment had the least biomass in root FW and DW. In tissue elemental analyses, both the pH 7.0 treatments showed lower concentrations than H5 in Cu, N, Mo, and Sr, and increased concentrations in Ba, Mg, Na, and Zn. There were no differences in Al, C, Ca, Fe, K, Mn, Ni, P, S, and Si concentrations among treatments (p = 0.05). The results from this experiment can be used to isolate the effects of pH and alkalinity in aquaponic conditions where pH and alkalinity will mimic HA7 conditions.
A Matrix-Based Structure for Vario-Scale Vector Representation over a Wide Range of Map Scales: The Case of River Network Data
Authors: Huang Lina , Ai Tinghua , Oosterom Peter Van , Yan Xiongfeng , Yang Min
Published: July 2017

The representation of vector data at variable scales has been widely applied in geographic information systems and map-based services. When the scale changes across a wide range, a complex generalization that involves multiple operations is required to transform the data. To present such complex generalization, we proposed a matrix model to combine different generalization operations into an integration. This study was carried on a set of river network data, where two operations, i.e., network pruning accompanied with river simplification, were hierarchically constructed as the rows and columns of a matrix. The correspondence between generalization operations and scale, and the scale linkage of multiple operations were also explicitly defined. In addition, we developed a vario-scale data structure to store the generalized river network data based on the proposed matrix. The matrix model was validated and assessed by a comparison with traditional methods that conduct generalization operations in sequence. It was shown that the matrix model enabled complex generalization with good generalization quality. Taking advantage of the corresponding vario-scale data structure, the river network data could be obtained at any arbitrary scale, and the vario-scale representation was achieved across a wide scale range.
The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance
Authors: Bascetta Lorenzo , Oliviero Arianna , D’Aurizio Romina , Evangelista Monica , Mercatanti Alberto , Pellegrini Marco , Marrocolo Francesca , Bracarda Sergio , Rizzo Milena
Published: July 2017

On the grounds that miRNAs present in the blood of prostate cancer (PCa) patients are released in the growth medium by PCa cells, it is conceivable that PCa cells resistant to docetaxel (DCT) (DCTR) will release miRNAs that may be found in PCa patients under DCT therapy if resistant PCa cells appear. We isolated DCTR clones respectively from 22Rv1 and DU-145 PCa cell lines and performed through next-generation sequencing (NGS) the miRNAs profiles of the released miRNAs. The analysis of the NGS data identified 105 and 1 miRNAs which were differentially released in the growth medium of the 22Rv1/DCTR and DU-145/DCTR clones, respectively. Using additional filters, we selected 12 and 1 miRNA more released by all 22Rv1/DCTR and DU-145/DCTR clones, respectively. Moreover, we showed that 6 of them were more represented in the growth medium of the DCTR cells than the ones of DCT-treated cells. We speculated that they have the pre-requisite to be tested as predictive biomarkers of the DCT resistance in PCa patients under DCT therapy. We propose the utilization of clones resistant to a given drug as in vitro model to identify the differentially released miRNAs, which in perspective could be tested as predictive biomarkers of drug resistance in tumor patients under therapy.
Exposure to Engineered Nanomaterials: Impact on DNA Repair Pathways
Authors: Singh Neenu , Nelson Bryant , Scanlan Leona , Coskun Erdem , Jaruga Pawel , Doak Shareen
Published: July 2017

Some engineered nanomaterials (ENMs) may have the potential to cause damage to the genetic material in living systems. The mechanistic machinery functioning at the cellular/molecular level, in the form of DNA repair processes, has evolved to help circumvent DNA damage caused by exposure to a variety of foreign substances. Recent studies have contributed to our understanding of the various DNA damage repair pathways involved in the processing of DNA damage. However, the vast array of ENMs may present a relatively new challenge to the integrity of the human genome; therefore, the potential hazard posed by some ENMs necessitates the evaluation and understanding of ENM-induced DNA damage repair pathways. This review focuses on recent studies highlighting the differential regulation of DNA repair pathways, in response to a variety of ENMs, and discusses the various factors that dictate aberrant repair processes, including intracellular signalling, spatial interactions and ENM-specific responses.
Understanding the Inguinal Sinus in Sheep (Ovis aries)—Morphology, Secretion, and Expression of Progesterone, Estrogens, and Prolactin Receptors
Authors: Alexandre-Pires Graça , Martins Catarina , Galvão António , Miranda Margarida , Silva Olga , Ligeiro Dário , Nunes Telmo , Ferreira-Dias Graça
Published: July 2017

Post-parturient behavior of mammalian females is essential for early parent–offspring contact. After delivery, lambs need to ingest colostrum for obtaining the related immunological protection, and early interactions between the mother and the lamb are crucial. Despite visual and auditory cues, olfactory cues are decisive in lamb orientation to the mammary gland. In sheep, the inguinal sinus is located bilaterally near the mammary gland as a skin pouch (IGS) that presents a gland that secretes a strong-smelling wax. Sheep IGS gland functions have many aspects under evaluation. The objective of the present study was to evaluate sheep IGS gland functional aspects and mRNA transcription and the protein expression of several hormone receptors, such as progesterone receptor (PGR), estrogen receptor 1 (ESR1), and 2 (ESR2) and prolactin receptor (PRLR) present. In addition, another aim was to achieve information about IGS ultrastructure and chemical compounds produced in this gland. All hormone receptors evaluated show expression in IGS during the estrous cycle (follicular/luteal phases), pregnancy, and the post-partum period. IGS secretion is rich in triterpenoids that totally differ from the surrounding skin. They might be essential substances for the development of an olfactory preference of newborns to their mothers.
Erythropoietin and Its Angiogenic Activity
Authors: Kimáková Patrícia , Solár Peter , Solárová Zuzana , Komel Radovan , Debeljak Nataša
Published: July 2017

Erythropoietin (EPO) is the main hematopoietic hormone acting on progenitor red blood cells via stimulation of cell growth, differentiation, and anti-apoptosis. However, its receptor (EPOR) is also expressed in various non-hematopoietic tissues, including endothelium. EPO is a pleiotropic growth factor that exhibits growth stimulation and cell/tissue protection on numerous cells and tissues. In this article we review the angiogenesis potential of EPO on endothelial cells in heart, brain, and leg ischemia, as well as its role in retinopathy protection and tumor promotion. Furthermore, the effect of EPO on bone marrow and adipose tissue is also discussed.
Tracing the Repertoire of Promiscuous Enzymes along the Metabolic Pathways in Archaeal Organisms
Authors: Martínez-Núñez Mario , Rodríguez-Escamilla Zuemy , Rodríguez-Vázquez Katya , Pérez-Rueda Ernesto
Published: July 2017

The metabolic pathways that carry out the biochemical transformations sustaining life depend on the efficiency of their associated enzymes. In recent years, it has become clear that promiscuous enzymes have played an important role in the function and evolution of metabolism. In this work we analyze the repertoire of promiscuous enzymes in 89 non-redundant genomes of the Archaea cellular domain. Promiscuous enzymes are defined as those proteins with two or more different Enzyme Commission (E.C.) numbers, according the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. From this analysis, it was found that the fraction of promiscuous enzymes is lower in Archaea than in Bacteria. A greater diversity of superfamily domains is associated with promiscuous enzymes compared to specialized enzymes, both in Archaea and Bacteria, and there is an enrichment of substrate promiscuity rather than catalytic promiscuity in the archaeal enzymes. Finally, the presence of promiscuous enzymes in the metabolic pathways was found to be heterogeneously distributed at the domain level and in the phyla that make up the Archaea. These analyses increase our understanding of promiscuous enzymes and provide additional clues to the evolution of metabolism in Archaea.
Mapping the Magnetic Anisotropy inside a Ni4 Cubane Spin Cluster Using Polarized Neutron Diffraction
Authors: Iasco Olga , Chumakov Yuri , Guégan Frédéric , Gillon Béatrice , Lenertz Marc , Bataille Alexandre , Jacquot Jean-François , Luneau Dominique
Published: July 2017

In this publication, we report on the study of the magnetic anisotropy of the cubane type tetranuclear cluster of Ni(II), [Ni4(L)4(MeOH)4] (H2L = salicylidene-2-ethanolamine; MeOH = methanol), by the means of angular-resolved magnetometry and polarized neutron diffraction (PND). We show that better than other usual characterization techniques—such as electron paramagnetic resonance spectroscopy (EPR) or SQUID magnetometry—only PND enables the full determination of the local magnetic susceptibility tensor of the tetranuclear cluster and those of the individual Ni(II) ions and the antiferromagnetic pairs they form. This allows highlighting that, among the two antiferromagnetic pairs in the cluster, one has a stronger easy-axis type anisotropy. This distinctive feature can only be revealed by PND measurements, stressing the remarkable insights that they can bring to the understanding of the magnetic properties of transition metals clusters.
Ultrafast Dynamics of Sb-Corroles: A Combined Vis-Pump Supercontinuum Probe and Broadband Fluorescence Up-Conversion Study
Authors: Zahn Clark , Stensitzki Till , Gerecke Mario , Berg Alexander , Mahammed Atif , Gross Zeev , Heyne Karsten
Published: July 2017

Corroles are a developing class of tetrapyrrole-based molecules with significant chemical potential and relatively unexplored photophysical properties. We combined femtosecond broadband fluorescence up-conversion and fs broadband Vis-pump Vis-probe spectroscopy to comprehensively characterize the photoreaction of 5,10,15-tris-pentafluorophenyl-corrolato-antimony(V)-trans-difluoride (Sb-tpfc-F2). Upon fs Soret band excitation at ~400 nm, the energy relaxed almost completely to Q band electronic excited states with a time constant of 500 ± 100 fs; this is evident from the decay of Soret band fluorescence at around 430 nm and the rise time of Q band fluorescence, as well as from Q band stimulated emission signals at 600 and 650 nm with the same time constant. Relaxation processes on a time scale of 10 and 20 ps were observed in the fluorescence and absorption signals. Triplet formation showed a time constant of 400 ps, with an intersystem crossing yield from the Q band to the triplet manifold of between 95% and 99%. This efficient triplet formation is due to the spin-orbit coupling of the antimony ion.
Novel Flexible Wearable Sensor Materials and Signal Processing for Vital Sign and Human Activity Monitoring
Authors: Servati Amir , Zou Liang , Wang Z. , Ko Frank , Servati Peyman
Published: July 2017

Advances in flexible electronic materials and smart textile, along with broad availability of smart phones, cloud and wireless systems have empowered the wearable technologies for significant impact on future of digital and personalized healthcare as well as consumer electronics. However, challenges related to lack of accuracy, reliability, high power consumption, rigid or bulky form factor and difficulty in interpretation of data have limited their wide-scale application in these potential areas. As an important solution to these challenges, we present latest advances in novel flexible electronic materials and sensors that enable comfortable and conformable body interaction and potential for invisible integration within daily apparel. Advances in novel flexible materials and sensors are described for wearable monitoring of human vital signs including, body temperature, respiratory rate and heart rate, muscle movements and activity. We then present advances in signal processing focusing on motion and noise artifact removal, data mining and aspects of sensor fusion relevant to future clinical applications of wearable technology.
Low-Carbon Transport Policy in Four ASEAN Countries: Developments in Indonesia, the Philippines, Thailand and Vietnam
Authors: Bakker Stefan , Dematera Contreras Kathleen , Kappiantari Monica , Tuan Nguyen , Guillen Marie , Gunthawong Gessarin , Zuidgeest Mark , Liefferink Duncan , Van Maarseveen Martin
Published: July 2017

Emerging countries in Southeast Asia are facing considerable challenges in addressing rising motorisation and its negative impact on air quality, traffic, energy security, liveability, and greenhouse gas emissions. This paper presents a comparative analysis of the approach and status of sustainable, low-carbon transport policy in ASEAN countries and identifies differences and similarities. The methodology is based on a taxonomy of policy components as developed by Howlett and Cashore. The data come from comprehensive country studies for Indonesia, the Philippines, Thailand and Vietnam and interviews with policymakers. We find that each country has a specific set of goals, objectives and targets that support sustainable transport, and, directly or indirectly, climate change mitigation. In terms of specific mechanisms and calibrations, which we analyse based on the Avoid−Shift−Improve approach, there are notable differences between the countries, for example in terms of fuel economy policy. Even though an initial response to climate change mitigation challenges is visible in these countries’ transport policies, much more effort is required to enable a transition to a transport system compatible with long-term climate change and sustainable development targets.
Quantitative Analysis of the Factors Influencing Soil Heavy Metal Lateral Migration in Rainfalls Based on Geographical Detector Software: A Case Study in Huanjiang County, China
Authors: Qiao Pengwei , Lei Mei , Guo Guanghui , Yang Jun , Zhou Xiaoyong , Chen Tongbin
Published: July 2017

Quantitative analysis of the factors influencing heavy metal migration could be useful for controlling heavy metal migration. In this paper, a geographical detector was used to calculate the contributions of and interactions among factors in Huanjiang County, South China, covering an area of 273 km2. In this paper, nine factors were analyzed. The results showed that, among these factors, soil type was the main factor influencing the migration of As, Pb and Cd; the other eight factors did not have big differences and were lower than soil type. In addition, there were obvious synergistic effects between the soil type and concentration of water-soluble heavy metals (CWS) and the concentration of water-insoluble heavy metals (CWI) and NDVI. Therefore, these factors of the study area were especially focused on. Furthermore, the results of the key factor identification and the high-risk region identification in the nine factors were reliable, based on the geographical detector software. Therefore, the geographical detector software could be used as an effective tool to quantitatively analyze the contribution of the factors, and identify the high-risk regions for the factors influencing soil heavy metal lateral migration in rainfalls.