bluuebunny/crossref_metadata_2025 huggingface dataset:
Posture Correction Therapy and Pelvic Floor Muscle Function Assessed by sEMG with Intravaginal Electrode and Manometry in Female with Urinary Incontinence
Authors: Jórasz Katarzyna , Truszczyńska-Baszak Aleksandra , Dąbek Aneta
Published: December 2022

Introduction: The aim of the study was to assess the influence of the implemented therapeutic programme, which consisted of body posture correction and of change of habits, on the pelvic floor muscle function in women with stress urinary incontinence. Material and methods: The 60 women were randomly divided into two groups: the study population and the clinical control group (subjects received envelopes with numbers of the group: 1- study population aged 38.3 ± 5.54, 2- clinical control group aged 35.5 ± 4.7. We used the following research methods: A personal questionnaire with subjects’ demographics and with questions related to the type of work, physical activity, childbirths and any issues related to the pelvic floor (pre-test), Pelvic floor muscle assessment with the use of the PERFECT Scheme and the Oxford scale palpation examination, sEMG with intravaginal electrode and manometry with an intravaginal probe—pre-test and post-test. Subjective assessment of body posture in the sagittal plane according to the McKenzie methodology. Results: In both groups, VRP (resting vaginal pressure) and resting PFM tension were significantly reduced. The strength and endurance of PFM, tension during MVC and VSP (intravaginal pressure during contraction) increased, with no difference between the groups. SUI decreased significantly, and quality of life improved significantly in both groups. Conclusions: Education of the pelvic floor and changing habits significantly affected the activity of PFM and improved the quality of life in the group of patients with SUI. The posture correction therapy with manual therapy and stretching exercises did not increase this effect.
Investigation of Spatial Coupling Coordination Development: Identifying Land System States from the Adaptation–Conflict Perspective
Authors: Wang Xingjia , Wang Dongyan , Gao Wanying , Lu Jiaxi , Jin Xiaotong
Published: December 2022

With the advancement of global urbanization, ecosystem conservation and sustainable land development have become major issues. In this context, the uncoordinated and imbalanced development of the land-centered human–environment system requires urgent attention, especially in rust belt cities that pose critical challenges to regional land system sustainability. Therefore, taking Changchun City from 1990 to 2020 as an example, we identified and evaluated the ecosystem service (ES) balance and land use conflict from the perspectives of internal support and external development pressure. Based on the land system adaptation and conflict results, a coupling coordination degree model (CCDM) was constructed to investigate the spatio-temporal characteristics of land system development. The results indicated that there was an obvious downward trend in the regional ES balance, while areas with higher ES balance were mainly distributed in the eastern ecologically sound areas, and southern built-up areas presented deficient ES balance levels (i.e., demand exceeding supply), with a significant expansion trend from 1990 to 2020. Land use conflict was especially prominent in areas experiencing rapid rural–urban transformation, and the hot spots expanded noticeably. The spatio-temporal differences in the coupling coordination degree of ES balance and land use conflict were significant, whereas the land system of the study area has always been dominated by a balanced development pattern throughout the research period, except for the urban center, which tended to be in a stage of uncoordinated development, with the ES balance blocked. These findings suggest that it is necessary to coordinate urban and adjacent regions through regionally integrated efforts to alleviate the ES imbalance. This research can provide a scientific reference for analyzing regional land system states, coordinating the sustainable spatial development of ecosystems, and implementing revitalization strategies to achieve win-win land system goals.
Interplay between Networking Capability and Hospital Performance in Indonesia’s Medical Tourism Sector
Authors: Ediansyah  , Arief Muhtosim , Hamsal Mohammad , Abdinagoro Sri Bramantoro
Published: December 2022

Medical tourism is an industry that is currently developing, but research that focuses on the analysis of supply and institutions as a unit in Indonesia is sparse. This research aims to investigate the variables of digital transformation and resource integration that influence hospital performance, considering the expansion of hospitals’ capacities for networking in medical tourism. A structural equation model is used to evaluate the hypothesis. According to our findings, digital transformation and resource integration both have a beneficial effect on networking capabilities. On the other hand, the implementation of digital transformation does not improve hospital performance. Digital transformation has a good link with hospital performance but has no statistically significant effect on hospital performance. In the meantime, the capabilities of resource integration and networking have a positive effect on the overall operation of hospitals. In a similar vein, the ecosystem of medical tourism helps to improve the connection between a hospital’s networking capabilities and its overall performance. It is anticipated that the findings of this study will serve as a reference for those in the policymaking and healthcare industries to develop medical tourism.
Constructing a Flood-Adaptive Ecological Security Pattern from the Perspective of Ecological Resilience: A Case Study of the Main Urban Area in Wuhan
Authors: Chen Hongyi , Liu Yanzhong , Hu Lin , Zhang Zuo , Chen Yong , Tan Yuchuan , Han Yufei
Published: December 2022

The frequent occurrence of floods in urban areas caused by climate change challenges urban resilience. This research aims to construct an ecological security pattern (ESP) that is adaptive to floods to enhance urban resilience in the hope that it will help cities cope with floods better. In this research, the main urban area of Wuhan (WUH) represents the study area. The lakes were selected as the ecological sources and the Soil Conservation Service-Curve Number (SCS-CN) model was used to calculate the runoff volume corresponding to each land type and, based on this, assign resistance values to the land types; as such, the land type surface is referred to as the runoff resistance surface, and the runoff resistance surface is then modified by ecosystem service capabilities. The Minimum Cumulative Resistance (MCR) model was used to extract the connecting corridors between the sources. This research plan includes 18 ecological sources, 10 key ecological corridors, and 22 potential ecological corridors, with a total length of about 344.21 km. Finally, it provides a two-axis and three-core urban ecological resilience optimization strategy for decision makers and a new approach for controlling floods in urban areas from the perspective of ecological resilience.
Optimizing Disaster Preparedness Planning for Minority Older Adults: One Size Does Not Fit All
Authors: Adepoju Omolola , Herrera Luz , Chae Minji , Han Daikwon
Published: December 2022

By 2050, one in five Americans will be 65 years and older. The growing proportion of older adults in the U.S. population has implications for many aspects of health including disaster preparedness. This study assessed correlates of disaster preparedness among community-dwelling minority older adults and explored unique differences for African American and Hispanic older adults. An electronic survey was disseminated to older minority adults 55+, between November 2020 and January 2021 (n = 522). An empirical framework was used to contextualize 12 disaster-related activities into survival and planning actions. Multivariate logistic regression models were stratified by race/ethnicity to examine the correlates of survival and planning actions in African American and Hispanic older adults, separately. We found that approximately 6 in 10 older minority adults did not perceive themselves to be disaster prepared. Medicare coverage was positively associated with survival and planning actions. Income level and prior experience with disaster were related to survival actions in the African American population. In conclusion, recognizing the gaps in disaster-preparedness in elderly minority communities can inform culturally sensitive interventions to improve disaster preparedness and recovery.
Central and Peripheral Fatigue in Recreational Trail Runners: A Pilot Study
Authors: Muñoz-Pérez Iker , Varela-Sanz Adrián , Lago-Fuentes Carlos , Navarro-Patón Rubén , Mecías-Calvo Marcos
Published: December 2022

Background: Understanding fatigue mechanisms is crucial for exercise performance. However, scientific evidence on non-invasive methods for assessing fatigue in trail running competitions is scarce, especially when vertical kilometer trail running races (VK) are considered. The main purpose of this study was to assess the autonomic nervous system (ANS) activity (i.e., central fatigue) and the state of muscle activation (i.e., peripheral fatigue) before and after a VK competition. Methods: A cross-sectional pilot study was performed. After applying inclusion/exclusion criteria, 8 recreational male trail runners (31.63 ± 7.21 yrs, 1.75 m ± 0.05 m, 70.38 ± 5.41 kg, BMI: 22.88 ± 0.48, running experience: 8.0 ± 3.63 yrs, weekly training volume: 58.75 ± 10.35 km) volunteered to participate and were assessed for both central (i.e., via heart rate variability, HRV) and peripheral (via tensiomyography, TMG) fatigue before and after a VK race. Results: After the VK, resting heart rate, RMSSD (p = 0.01 for both) and SDNN significantly decreased (p = 0.02), while the stress score and the sympathetic-parasympathetic ratio increased (p = 0.01 and p = 0.02, respectively). The TMG analyses suggest that runners already suffered peripheral fatigue before the VK and that 20–30 min are enough for muscular recovery after the race. In summary, our data suggest that participants experienced a pre-competition fatigue status. Further longitudinal studies are necessary to investigate the mechanisms underlying fatigue during trail running races, while training periodization and tapering strategies could play a key role for minimizing pre-competition fatigue status.
Friendly Residential Environments That Generate Autonomy in Older Persons
Authors: Segura Cardona Alejandra , Cardona Arango Doris , Segura Cardona Angela , Robledo Marín Carlos , Muñoz Rodríguez Diana
Published: December 2022

Objective: This study aimed to explore the housing and residential environment conditions associated with functional autonomy in older persons. Methods: A quantitative cross-sectional study was conducted, including 175 individuals over the age of 60. Participants were non-institutionalized urban residents of Medellín, Colombia, selected by random two-stage sampling (neighborhoods and blocks). Analysis was done according to functional autonomy of action (dependent variable); and demographic conditions, housing, and the physical and social environment suggested by the World Health Organization (WHO) in the strategy of age-friendly cities (independent variables). Univariate, bivariate, and multivariate analyses were performed with these variables, where the odds ratio (OR), association hypothesis test, and confidence intervals were estimated, using logistic regression models. Results: 89.7% of older persons had moderate physical performance. The performance of intergenerational activities (OR = 5.28) and community actions (OR = 11.28) were part of social environments. The adaptations in public transport (OR = 90.33), sanitary services (OR = 4.1), and lighting in parks (OR = 19.9) of the physical environment were the associations found with functional autonomy. Conclusions: Exploring how the physical and social environments surrounding housing are associated with the functional performance of older persons can generate useful information to support public health and city infrastructure strategies that improve their physical performance and maintain autonomy.
Parents’ Experiences Using Digital Health Technologies in Paediatric Overweight and Obesity Support: An Integrative Review
Authors: Fidjeland Torbjørg Goa , Øen Kirsten Gudbjørg
Published: December 2022

This study aimed to explore parents’ perspectives regarding the use of digital health technologies in paediatric overweight and obesity weight support. A systematic search in ‘Academic Search Premier’, ‘British Nursing Index’, ‘Cumulative Index to Nursing and Allied Health’, and ‘Health Research Premium Collection’ was conducted in November 2021. Inclusion criteria were English or Nordic peer-reviewed articles published after 2010, overweight and obese children aged 2–10, weight support using digital health technologies, and parental experiences examined. An integrative review was conducted using Joanna Briggs Institute quality appraisal checklists and a constant comparison analysis method. In total, 14 articles were analysed that included SMS, telephone, apps, websites, and social media as the main technology groups. A limitation of this review was the heterogenicity of the studies. The results indicate that parents, mostly mothers, had a positive experience, finding technologies helpful and easy to use, and expressed optimism toward future use. An option for interacting with others and the possibility of personalising support were enjoyed or requested. In conclusion, digital health technologies can be a suitable tool to empower the parents of children with overweight and obesity concerns, whose perspective should be considered during the design and support phases.
The Impact of Nurse Health-Coaching Strategies on Cognitive—Behavioral Outcomes in Older Adults
Authors: Potempa Kathleen , Butterworth Susan , Flaherty-Robb Marna , Calarco Margaret , Marriott Deanna , Ghosh Bidisha , Gabarda Amanda , Windsor Jordan , Potempa Stacia , Laughlin Candia , Harden Karen , Schmidt Patricia , Ellis Alexis , Furspan Philip
Published: December 2022

The practice of nurse health coaching (NHC) draws from the art and science of nursing, behavioral sciences, and evidence-based health-coaching methods. This secondary analysis of the audio-recorded natural language of participants during NHC sessions of our recent 8-week RCT evaluates improvement over time in cognitive–behavioral outcomes: change talk, resiliency, self-efficacy/independent agency, insight and pattern recognition, and building towards sustainability. We developed a measurement tool for coding, Indicators of Health Behavior Change (IHBC), that was designed to allow trained health-coach experts to assess the presence and frequency of the indicators in the natural language content of participants. We used a two-step method for randomly selecting the 20 min audio-recorded session that was analyzed at each time point. Fifty-six participants had high-quality audio recordings of the NHC sessions. Twelve participants were placed in the social determinants of health (SDH) group based on the following: low income (<USD 20,000/year), early-onset hypertension, and social disadvantages. Our analyses significantly improved change talk and the other four factors over time. Our factor analyses indicated two distinct factors at each measurement point of the study, demonstrating the stability of the outcome measures over time. Our newly developed measurement tool, IHBC, proved stable in structure over time and sensitive to change. This NHC program shows promise in improving cognitive–behavioral indicators associated with health behavior change in both non-SDH and SDH individuals.
Investor Sentiment Index: A Systematic Review
Authors: Prasad Sourav , Mohapatra Sabyasachi , Rahman Molla Ramizur , Puniyani Amit
Published: December 2022

The Investor Sentiment Index (ISI) is widely regarded as a useful measure to gauge the overall mood of the market. Investor panic may result in contagion, causing failure in financial markets. Market participants widely use the ISI indicator to understand price fluctuations and related opportunities. As a result, it is imperative to systematically review the compiled literature on the subject. In addition to reviewing past studies on the ISI, this paper attempts a bibliometric analysis (BA) to understand any related publications. We systematically review over 100 articles and carry out a BA on a set of information based on the publication year, the journal, the countries/territories, the deployed statistical tools and techniques, a citation analysis, and a content analysis. This analysis further strengthens the study by establishing interesting findings. Most articles use the Baker and Wurgler index and text-based sentiment analysis. However, an Internet-search-based ISI was also used in a few of the studies. The results reveal the lack of direct measures or a robust qualitative approach in constructing the ISI. The findings further indicate a vast research gap in emerging economies, such as India’s. This study had no limit on the period for inclusion and exclusion. We believe that our current work is a seminal study, jointly involving a systematic literature review and BA, that will enormously facilitate academicians and practitioners working on the ISI.
Recognition of Intersection Traffic Regulations from Crowdsourced Data
Authors: Zourlidou Stefania , Sester Monika , Hu Shaohan
Published: December 2022

In this paper, a new method is proposed to detect traffic regulations at intersections using GPS traces. The knowledge of traffic rules for regulated locations can help various location-based applications in the context of Smart Cities, such as the accurate estimation of travel time and fuel consumption from a starting point to a destination. Traffic regulations as map features, however, are surprisingly still largely absent from maps, although they do affect traffic flow which, in turn, affects vehicle idling time at intersections, fuel consumption, CO2 emissions, and arrival time. In addition, mapping them using surveying equipment is costly and any update process has severe time constraints. This fact is precisely the motivation for this study. Therefore, its objective is to propose an automatic, fast, scalable, and inexpensive way to identify the type of intersection control (e.g., traffic lights, stop signs). A new method based on summarizing the collective behavior of vehicle crossing intersections is proposed. A modification of a well-known clustering algorithm is used to detect stopping and deceleration episodes. These episodes are then used to categorize vehicle crossing of intersections into four possible traffic categories (p1: free flow, p2: deceleration without stopping events, p3: only one stopping event, p4: more than one stopping event). The percentages of crossings of each class per intersection arm, together with other speed/stop/deceleration features, extracted from trajectories, are then used as features to classify the intersection arms according to their traffic control type (dynamic model). The classification results of the dynamic model are compared with those of the static model, where the classification features are extracted from OpenStreetMap. Finally, a hybrid model is also tested, where a combination of dynamic and static features is used, which outperforms the other two models. For each of the three models, two variants of the feature vector are tested: one where only features associated with a single intersection arm are used (one-arm model) and another where features also from neighboring intersection arms of the same intersection are used to classify an arm (all-arm model). The methodology was tested on three datasets and the results show that all-arm models perform better than single-arm models with an accuracy of 95% to 97%.
Canopy Assessment of Cycling Routes: Comparison of Videos from a Bicycle-Mounted Camera and GPS and Satellite Imagery
Authors: Bourassa Albert , Apparicio Philippe , Gelb Jérémy , Boisjoly Geneviève
Published: December 2022

Many studies have proven that urban greenness is an important factor when cyclists choose a route. Thus, detecting trees along a cycling route is a major key to assessing the quality of cycling routes and providing further arguments to improve ridership and the better design of cycling routes. The rise in the use of video recordings in data collection provides access to a new point of view of a city, with data recorded at eye level. This method may be superior to the commonly used normalized difference vegetation index (NDVI) from satellite imagery because satellite images are costly to obtain and cloud cover sometimes obscures the view. This study has two objectives: (1) to assess the number of trees along a cycling route using software object detection on videos, particularly the Detectron2 library, and (2) to compare the detected canopy on the videos to other canopy data to determine if they are comparable. Using bicycles installed with cameras and GPS, four participants cycled on 141 predefined routes in Montréal over 87 h for a total of 1199 km. More than 300,000 images were extracted and analyzed using Detectron2. The results show that the detection of trees using the software is accurate. Moreover, the comparison reveals a strong correlation (>0.75) between the two datasets. This means that the canopy data could be replaced by video-detected trees, which is particularly relevant in cities where open GIS data on street vegetation are not available.
Silver Nanoparticle Synthesis via Photochemical Reduction with Sodium Citrate
Authors: Pascu Bogdan , Negrea Adina , Ciopec Mihaela , Duteanu Narcis , Negrea Petru , Bumm Lloyd A. , Grad (mBuriac) Oana , Nemeş Nicoleta Sorina , Mihalcea Cătălina , Duda-Seiman Daniel Marius
Published: December 2022

The aim of this paper is to provide a simple and efficient photoassisted approach to synthesize silver nanoparticles, and to elucidate the role of the key factors (synthesis parameters, such as the concentration of TSC, irradiation time, and UV intensity) that play a major role in the photochemical synthesis of silver nanoparticles using TSC, both as a reducing and stabilizing agent. Concomitantly, we aim to provide an easy way to evaluate the particle size based on Mie theory. One of the key advantages of this method is that the synthesis can be “activated” whenever or wherever silver nanoparticles are needed, by premixing the reactants and irradiating the final solution with UV radiation. UV irradiance was determined by using Keitz’s theory. This argument has been verified by premixing the reagents and deposited them in an enclosed space (away from sunlight) at 25 °C, then checking them for three days. Nothing happened, unless the sample was directly irradiated by UV light. Further, obtained materials were monitored for 390 days and characterized using scanning electron microscopy, UV-VIS, and transmission electron microscopy.
Macrophages Release Extracellular Vesicles of Different Properties and Composition Following Exposure to Nanoparticles
Authors: Deville Sarah , Garcia Romeu Hector , Oeyen Eline , Mertens Inge , Nelissen Inge , Salvati Anna
Published: December 2022

Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in intercellular communication, for instance, in the context of the immune response. Macrophages are known to release extracellular vesicles in response to different stimuli, and changes in their size, number, and composition may provide important insights into the responses induced. Macrophages are also known to be highly efficient in clearing nanoparticles, when in contact with them, and in triggering the immune system. However, little is known about how the nature and composition of the vesicles released by these cells may vary upon nanoparticle exposure. In order to study this, in this work, alveolar-like macrophages were exposed to a panel of nanoparticles with varying surface and composition, including amino-modified and carboxylated polystyrene and plain silica. We previously showed that these nanoparticles induced very different responses in these cells. Here, experimental conditions were carefully tuned in order to separate the extracellular vesicles released by the macrophages several hours after exposure to sub-toxic concentrations of the same nanoparticles. After separation, different methods, including high-sensitivity flow cytometry, TEM imaging, Western blotting, and nanoparticle tracking analysis, were combined in order to characterize the extracellular vesicles. Finally, proteomics was used to determine their composition and how it varied upon exposure to the different nanoparticles. Our results show that depending on the nanoparticles’ properties. The macrophages produced extracellular vesicles of varying number, size, and protein composition. This indicates that macrophages release specific signals in response to nanoparticles and overall suggests that extracellular vesicles can reflect subtle responses to nanoparticles and nanoparticle impact on intercellular communication.
Involvement of Diamine Oxidase in Modification of Plasma Membrane Proton Pump Activity in Cucumis sativus L. Seedlings under Cadmium Stress
Authors: Janicka Małgorzata , Reda Małgorzata , Napieraj Natalia , Michalak Adrianna , Jakubowska Dagmara , Kabała Katarzyna
Published: December 2022

Cucumber (Cucumis sativus L.) is a crop plant being the third most-produced vegetable developed as a new model plant. Heavy metal pollution is a serious global problem that affects crop production. An industrial activity has led to high emissions of Cd into the environment. Plants realize adaptive strategies to diminish the toxic effects of Cd. They can remove excess toxic ions of heavy metals from the cytoplasm to the outside of cells using the metal/proton antiport. The proton gradient needed for the action of the antiporter is generated by the plasma membrane (PM) H+-ATPase (EC 3.6.3.14). We have shown that treatment of cucumber plants with Cd stimulated the diamine oxidase (DAO, EC 1.4.3.6) activity in roots. Under cadmium stress, the PM H+-ATPase activity also increased in cucumber seedlings. The stimulating effect of Cd on the PM H+-ATPase activity and expression of three genes encoding this enzyme (CsHA2, CsHA4, CsHA8) was reduced by aminoguanidine (AG, a DAO inhibitor). Moreover, we have observed that H2O2 produced by DAO promotes the formation of NO in the roots of seedlings. The results presented in this work showed that DAO may be an element of the signal transduction pathway, leading to enhanced PM H+-ATPase activity under cadmium stress.
Toll-like Receptors and Celiac Disease
Authors: Talipova Diana , Smagulova Aiganym , Poddighe Dimitri
Published: December 2022

Celiac disease (CD) is an immune-mediated disorder triggered by dietary gluten intake in some genetically predisposed individuals; however, the additional non-HLA-related genetic factors implicated in CD immunopathogenesis are not well-defined. The role of the innate immune system in autoimmunity has emerged in the last few years. Genetic polymorphisms of some pattern-recognition receptors, including toll-like receptors (TLRs), have been associated with several autoimmune disorders. In this review, we summarize and discuss the evidence from basic research and clinical studies as regards the potential role of TLRs in CD immunopathogenesis. The evidence supporting the role of TLRs in CD immunopathogenesis is limited, especially in terms of basic research. However, differences in the expression and activation of TLRs between active CD patients from one side, and controls and treated CD patients from the other side, have been described in some clinical studies. Therefore, TLRs may be part of those non-HLA-related genetic factors implicated in CD etiopathogenesis, considering their potential role in the interaction between the host immune system and some environmental factors (including viral infections and gut microbiota), which are included in the list of candidate agents potentially contributing to the determination of CD risk in genetically predisposed individuals exposed to dietary gluten intake. Further basic research and clinical studies focused on TLRs in the context of CD and other gluten-related disorders are needed.
A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti-Pseudomonas aeruginosa Infection in Zebrafish
Authors: Chen Yan-Chao , Qiu Wanlei , Zhang Weibin , Zhang Jingrong , Chen Roushi , Chen Fangyi , Wang Ke-Jian
Published: December 2022

New antimicrobial agents are urgently needed to address the increasing emergence and dissemination of multidrug-resistant bacteria. In the study, a chemically synthesized truncated peptide containing 22-amino acids derived from a C-type lectin homolog SpCTL6 of Scylla paramamosain was screened and found to exhibit broad-spectrum antimicrobial activity, indicating that it is an antimicrobial peptide (AMP), named Sp-LECin. Sp-LECin possessed the basic characteristics of most cationic AMPs, such as positive charge (+4) and a relatively high hydrophobicity (45%). After treatment with Sp-LECin, the disruption of microbial membrane integrity and even leakage of cellular contents was observed by scanning electron microscopy (SEM). In addition, Sp-LECin could bind lipopolysaccharide (LPS), increase the outer and inner membrane permeability and induce reactive oxygen species (ROS) production, ultimately leading to the death of Pseudomonas aeruginosa. Furthermore, Sp-LECin exhibited potent anti-biofilm activity against P. aeruginosa during both biofilm formation and maturation. Notably, Sp-LECin had no obvious cytotoxicity and could greatly improve the survival of P. aeruginosa-infected zebrafish, by approximately 40% over the control group after 72 h of treatment. This study indicated that Sp-LECin is a promising antibacterial agent with the potential to be used against devastating global pathogen infections such as P. aeruginosa.
Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms
Authors: Avgeros Chrysostomos , Patsatsi Aikaterini , Dimitriadis Dimitrios , Malousi Andigoni , Koletsa Triantafyllia , Papathemeli Despoina , Syrnioti Antonia , Avgerou Paraskevi , Lazaridou Elizabeth , Tzimagiorgis Georgios , Georgiou Elisavet
Published: December 2022

Diagnosis of Mycosis Fungoides (MF) may be challenging, due to its polymorphic nature. The use of miRNAs as biomarkers to assist in diagnosis has been investigated, mainly in skin lesion biopsies. The purpose of this study is to evaluate the plasma levels of miR-146a and miR-155 in MF patients and to investigate their association with SNPs of their genes. Plasma miRNAs were quantified by RT-qPCR. Genomic DNA was used for SNPs’ genotyping by Sanger sequencing. Plasma levels of miR-146a and miR-155 were significantly higher in patients vs. controls, in early MF patients vs. controls, and in advanced vs. early MF patients. Both miRNAs’ levels were significantly higher in stage IIB vs. early-stage patients. miR-155 plasma levels were significantly higher in patients with skin tumors or erythroderma. CC genotype (rs2910164 C>G) was significantly more frequent in healthy controls and associated with lower MF risk and lower miR-146a levels. The AA genotype (rs767649 T>A) was significantly more frequent in patients and correlated with increased MF risk and increased miR-155 levels. The combination of GG+AA was only detected in patients and was correlated with higher MF susceptibility. Increased mir-146a and mir-155 plasma levels in MF is an important finding to establish putative noninvasive biomarkers. The presence of SNPs is closely associated with miRs’ expression, and possibly with disease susceptibility.
Enhanced Bactericidal Effect of Calcinated Mg–Fe Layered Double Hydroxide Films Driven by the Fenton Reaction
Authors: Chen Lei , Yin Yijia , Jian Linjia , Han Xianglong , Zhao Xuefeng , Wang Donghui
Published: December 2022

Osteogenic and antibacterial abilities are the permanent pursuit of titanium (Ti)-based orthopedic implants. However, it is difficult to strike the right balance between these two properties. It has been proved that an appropriate alkaline microenvironment formed by Ti modified by magnesium–aluminum layered double hydroxides (Mg–Al LDHs) could achieve the selective killing of bacteria and promote osteogenesis. However, the existence of Al induces biosafety concerns. In this study, iron (Fe), an essential trace element in the human body, was used to substitute Al, and a calcinated Mg–Fe LDH film was constructed on Ti. The results showed that a proper local alkaline environment created by the constructed film could enhance the antibacterial and osteogenic properties of the material. In addition, the introduction of Fe promoted the Fenton reaction and could produce reactive oxygen species in the infection environment, which might further strengthen the in vivo bactericidal effect.
Combined Therapy with Simvastatin- and Coenzyme-Q10-Loaded Nanoparticles Upregulates the Akt-eNOS Pathway in Experimental Metabolic Syndrome
Authors: Şaman Ezgi , Cebova Martina , Barta Andrej , Koneracka Martina , Zavisova Vlasta , Eckstein-Andicsova Anita , Danko Martin , Mosnacek Jaroslav , Pechanova Olga
Published: December 2022

In addition to their LDL-cholesterol-lowering effect, statins have pleiotropic beneficial effects on the cardiovascular system. However, long-term treatment with statins may be associated with serious side effects. With the aim to make statin therapy more effective, we studied the effects of simvastatin- and coenzyme-Q10-loaded polymeric nanoparticles on the lipid profile and nitric oxide (NO)/reactive oxygen species (ROS) balance in the heart and aorta of adult male obese Zucker rats. The rats were divided into an untreated group, a group treated with empty nanoparticles, and groups treated with simvastatin-, coenzyme Q10 (CoQ10)-, or a combination of simvastatin- and CoQ10-loaded nanoparticles (SIMV+CoQ10). After 6 weeks, the lipid profile in the plasma and the concentration of conjugated dienes in the liver were determined. Nitric oxide synthase (NOS) activity, Akt, endothelial NOS (eNOS), phosphorylated eNOS (p-eNOS), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, and nuclear factor kappaB (NF-kappaB) protein expressions were measured in the heart and aorta. All simvastatin, CoQ10, and SIMV+CoQ10 treatments decreased plasma LDL levels, but only the combined SIMV+CoQ10 treatment increased NOS activity and the expression of Akt, eNOS, and p-eNOS in both the heart and the aorta. Interestingly, NADPH oxidase in the heart and NF-kappaB protein expression in the aorta were decreased by all treatments, including nanoparticles alone. In conclusion, only combined therapy with SIMV- and CoQ10-loaded nanoparticles increased NOS activity and upregulated the Akt-eNOS pathway in obese Zucker rats, which may represent a promising tool for the treatment of cardiometabolic diseases.
Microarray Expression Profile of Myricetin-Treated THP-1 Macrophages Exhibits Alterations in Atherosclerosis-Related Regulator Molecules and LXR/RXR Pathway
Authors: Huwait Etimad , Almassabi Rehab , Almowallad Sanaa , Saddeek Salma , Karim Sajjad , Kalamegam Gauthaman , Mirza Zeenat
Published: December 2022

Atherosclerosis is a chronic inflammation characterized by macrophage infiltration, lipid deposition, and arterial wall thickening. Prevention of atherosclerosis by nutraceuticals is gaining attention. Myricetin, a dietary flavonol, is claimed to possess anti-atherosclerosis properties. We studied myricetin’s effect on the atherosclerosis-associated molecular mechanism. Cytotoxicity and proliferation testing to check the viability of myricetin-treated THP-1 macrophages and monocyte migration study in the presence and absence of myricetin was performed. The whole transcriptome analysis was conducted using the Affymetrix microarray platform. The Partek genomics suite for detecting differentially expressed genes (DEGs) and ingenuity pathway analysis was used to identify canonical pathways. Cytotoxicity assays exhibited no significant toxicity in THP-1 macrophages treated with different myricetin concentrations (10–200 μM). Genome-wide expression profiling revealed 58 DEGs (53 upregulated and 5 downregulated) in myricetin-treated THP-1 macrophages. Pathway analysis revealed inhibition of LXR/RXR activation and angiogenesis inhibition by thrombospondin-1 and activated phagocytosis in myricetin-treated THP-1 macrophages. The cytotoxicity assay shows myricetin as a safe phytochemical. In vitro and in silico pathway studies on THP-1 macrophages showed that they can inhibit THP-1 monocyte migration and alter the cholesterol efflux mediated via LXR/RXR signaling. Therefore, myricetin could help in the prevention of cell infiltration in atherosclerotic plaque with reduced risk of stroke or brain damage.
Molecular Mechanisms of Na-Cl Cotransporter in Relation to Hypertension in Chronic Kidney Disease
Authors: Liang Lijuan , Shimosawa Tatsuo
Published: December 2022

Chronic kidney disease (CKD) is a common clinical disease with an increasing incidence, affecting 10 to 15% of the world’s population. Hypertension is the most common and modifiable risk factor for preventing adverse cardiovascular outcomes in patients with CKD. A survey from developed countries shows that 47% of hypertensive patients over the age of 20 have uncontrolled blood pressure (BP), and the control rate is even lower in developing countries. CKD is both a common cause of uncontrolled hypertension and a risk factor for altered sequelae. In particular, studies have demonstrated that abnormal blood-pressure patterns in CKD patients, such as non-dipping-blood-pressure patterns, are associated with a significantly increased risk of cardiovascular (CV) disease. The distal convoluted tubule (DCT) is a region of the kidney, and although only 5–10% of the sodium (Na+) filtered by the glomerulus is reabsorbed by DCT, most studies agree that Na-Cl cotransporter (NCC) in human, rabbit, mouse, and rat kidneys is the most important route of sodium reabsorption across the DCT for maintaining the homeostasis of sodium. The regulation of NCC involves a large and complex network structure, including certain physiological factors, kinases, scaffold proteins, transporter phosphorylation, and other aspects. This regulation network includes various levels. Naturally, cross-talk between the components of this system must occur in order to relay the important signals to the transporter to play its role. Knowledge of the mechanisms regulating NCC activation is critical for understanding and treating hypertension and CKD. Previous studies from our laboratory have investigated the mechanisms through which NCC is activated in several different models. In the following sections, we review the literature on the mechanisms of NCC in relation to hypertension in CKD.
Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
Authors: Flasz Barbara , Dziewięcka Marta , Ajay Amrendra K. , Tarnawska Monika , Babczyńska Agnieszka , Kędziorski Andrzej , Napora-Rutkowski Łukasz , Ziętara Patrycja , Świerczek Ewa , Augustyniak Maria
Published: December 2022

The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms.
Novel Arylpiperazine Derivatives of Salicylamide with α1-Adrenolytic Properties Showed Antiarrhythmic and Hypotensive Properties in Rats
Authors: Żmudzka Elżbieta , Lustyk Klaudia , Siwek Agata , Wolak Małgorzata , Gałuszka Adam , Jaśkowska Jolanta , Kołaczkowski Marcin , Sapa Jacek , Pytka Karolina
Published: December 2022

Cardiovascular diseases remain one of the leading causes of death worldwide. Unfortunately, the available pharmacotherapeutic options have limited effectiveness. Therefore, developing new drug candidates remains very important. We selected six novel arylpiperazine alkyl derivatives of salicylamide to investigate their cardiovascular effects. Having in mind the beneficial role of α1-adrenergic receptors in restoring sinus rhythm and regulating blood pressure, first, using radioligand binding assays, we evaluated the affinity of the tested compounds for α-adrenergic receptors. Our experiments revealed their high to moderate affinity for α1- but not α2-adrenoceptors. Next, we aimed to determine the antiarrhythmic potential of novel derivatives in rat models of arrhythmia induced by adrenaline, calcium chloride, or aconitine. All compounds showed potent prophylactic antiarrhythmic activity in the adrenaline-induced arrhythmia model and no effects in calcium chloride- or aconitine-induced arrhythmias. Moreover, the tested compounds demonstrated therapeutic antiarrhythmic activity, restoring a normal sinus rhythm immediately after the administration of the arrhythmogen adrenaline. Notably, none of the tested derivatives affected the normal electrocardiogram (ECG) parameters in rodents, which excludes their proarrhythmic potential. Finally, all tested compounds decreased blood pressure in normotensive rats and reversed the pressor response to methoxamine, suggesting that their hypotensive mechanism of action is connected with the blockade of α1-adrenoceptors. Our results confirm the antiarrhythmic and hypotensive activities of novel arylpiperazine derivatives and encourage their further investigation as model structures for potential drugs.
Melatonin Is Neuroprotective in Escherichia coli Meningitis Depending on Intestinal Microbiota
Authors: Zhang Dong , Xu Shu , Wu Hucong , Liu Jiaqi , Wang Yiting , Zhu Guoqiang
Published: December 2022

Avian meningitis Escherichia coli (E. coli) can cause acute bacterial meningitis which threatens poultry health, causes great economic losses in the poultry industry, and has recently been speculated as a potential zoonotic pathogen. Melatonin can counteract bacterial meningitis-induced disruption of the blood–brain barrier (BBB), neuroinflammation, and reduce mortality. There are increasing data showing that melatonin’s beneficial effects on bacterial meningitis are associated with intestinal microbiota. In this study, our data showed that melatonin alleviated neurological symptoms, enhanced survival rate, protected the integrity of the BBB, reduced the bacterial load in various tissues and blood, and inhibited inflammation and neutrophil infiltration of brain tissue in an APEC TW-XM-meningitis mice model. The results of 16S rRNA showed that melatonin pretreatment significantly maintained the composition of intestinal microbiota in APEC-meningitis mice. The abundance and diversity of intestinal microbiota were disturbed in APEC TW-XM-meningitis mice, with a decreased ratio of Firmicutes to Bacteroides and an increased the abundance of Proteobacteria. Melatonin pretreatment could significantly improve the composition and abundance of harmful bacteria and alleviate the decreased abundance of beneficial bacteria. Importantly, melatonin failed to affect the meningitis neurologic symptoms caused by APEC TW-XM infection in antibiotic-pretreated mice. In conclusion, the results suggest that melatonin can effectively prevent meningitis induced by APEC TW-XM infection in mice, depending on the intestinal microbiota. This finding is helpful to further explore the specific target mechanism of melatonin-mediated intestinal microbiota in the prevention of and protection against Escherichia coli meningitis.
Anti-Obesity Effects of Isorhamnetin and Isorhamnetin Conjugates
Authors: González-Arceo Maitane , Gomez-Lopez Iván , Carr-Ugarte Helen , Eseberri Itziar , González Marcela , Cano M. Pilar , Portillo María P. , Gómez-Zorita Saioa
Published: December 2022

Isorhamnetin is a plant-derived secondary metabolite which belongs to the family of flavonoids. This review summarises the main outcomes described in the literature to date, regarding the effects of isorhamnetin on obesity from in vitro and in vivo studies. The studies carried out in pre-adipocytes show that isorhamnetin is able to reduce adipogenesis at 10 μM or higher doses and that these effects are mediated by Pparγ and by Wnt signalling pathway. Very few studies addressed in rodents are available so far. It seems that treatment periods longer than two weeks are needed by isorhamnetin and its glycosides to be effective as anti-obesity agents. Nevertheless, improvements in glycaemic control can be observed even in short treatments. Regarding the underlying mechanisms of action, although some contradictory results have been found, reductions in de novo lipogenesis and fatty acid uptake could be proposed. Further research is needed to increase the scientific evidence referring to this topic; studies in animal models are essential, as well as randomised clinical trials to determine whether the positive results observed in animals could also be found in humans, in order to determine if isorhamnetin and its glycosides can represent a real tool against obesity.
The Hippocampal Response to Acute Corticosterone Elevation Is Altered in a Mouse Model for Angelman Syndrome
Authors: Viho Eva M. G. , Punt A. Mattijs , Distel Ben , Houtman René , Kroon Jan , Elgersma Ype , Meijer Onno C.
Published: December 2022

Angelman Syndrome (AS) is a severe neurodevelopmental disorder, caused by the neuronal absence of the ubiquitin protein ligase E3A (UBE3A). UBE3A promotes ubiquitin-mediated protein degradation and functions as a transcriptional coregulator of nuclear hormone receptors, including the glucocorticoid receptor (GR). Previous studies showed anxiety-like behavior and hippocampal-dependent memory disturbances in AS mouse models. Hippocampal GR is an important regulator of the stress response and memory formation, and we therefore investigated whether the absence of UBE3A in AS mice disrupted GR signaling in the hippocampus. We first established a strong cortisol-dependent interaction between the GR ligand binding domain and a UBE3A nuclear receptor box in a high-throughput interaction screen. In vivo, we found that UBE3A-deficient AS mice displayed significantly more variation in circulating corticosterone levels throughout the day compared to wildtypes (WT), with low to undetectable levels of corticosterone at the trough of the circadian cycle. Additionally, we observed an enhanced transcriptomic response in the AS hippocampus following acute corticosterone treatment. Surprisingly, chronic corticosterone treatment showed less contrast between AS and WT mice in the hippocampus and liver transcriptomic responses. This suggests that UBE3A limits the acute stimulation of GR signaling, likely as a member of the GR transcriptional complex. Altogether, these data indicate that AS mice are more sensitive to acute glucocorticoid exposure in the brain compared to WT mice. This suggests that stress responsiveness is altered in AS which could lead to anxiety symptoms.