bluuebunny/crossref_metadata_2025 huggingface dataset:
SAD-ARGRU: A Metro Passenger Flow Prediction Model for Graph Residual Gated Recurrent Networks
Authors: Zhang Jilin , Chen Yanling , Zhang Shuaifeng , Zhang Yang
Published: April 2024

This paper proposes a graph residual gated recurrent network subway passenger flow prediction model considering the flat-peak characteristics, which firstly proposes the use of an adaptive density clustering method, which is capable of dynamically dividing the flat-peak time period of subway passenger flow. Secondly, this paper proposes graph residual gated recurrent network, which uses a graph convolutional network fused with a residual network and combined with a gated recurrent network, to simultaneously learn the temporal and spatial characteristics of passenger flow. Finally, this paper proposes to use the spatial attention mechanism to learn the spatial features around the subway stations, construct the spatial local feature components, and fully learn the spatial features around the stations to realize the local quantization of the spatial features around the subway stations. The experimental results show that the graph residual gated recurrent network considering the flat-peak characteristics can effectively improve the prediction performance of the model, and the method proposed in this paper has the highest prediction accuracy when compared with the traditional prediction model.
Studies on Ionic Flows via Poisson–Nernst–Planck Systems with Bikerman’s Local Hard-Sphere Potentials under Relaxed Neutral Boundary Conditions
Authors: Liu Xiangshuo , Zhang Lijun , Zhang Mingji
Published: April 2024

We examine the qualitative properties of ionic flows through ion channels via a quasi-one-dimensional Poisson–Nernst–Planck model under relaxed neutral boundary conditions. Bikerman’s local hard-sphere potential is included in the model to account for finite ion size effects. Our main interest is to examine the boundary layer effects (due to the relaxation of electroneutrality boundary conditions) on both individual fluxes and current–voltage relations systematically. Critical values of potentials are identified that play significant roles in studying internal dynamics of ionic flows. It turns out that the finite ion size can either enhance or reduce the ionic flow under different nonlinear interplays between the physical parameters in the system, particularly, boundary concentrations, boundary potentials, boundary layers, and finite ion sizes. Much more rich dynamics of ionic flows through membrane channels is observed.
Multi-Pursuer and One-Evader Evasion Differential Game with Integral Constraints for an Infinite System of Binary Differential Equations
Authors: Kazimirova Ruzakhon , Ibragimov Gafurjan , Pansera Bruno Antonio , Ibragimov Abdulla
Published: April 2024

In the Hilbert space l2, a differential evasion game involving multiple pursuers is considered. Integral constraints are imposed on player control functions. The pursuers are tasked with bringing the state of a system back to the origin of l2, while the evader simultaneously tries to avoid it. It is assumed that the energy of the evader is greater than the total energy of the pursuers. In this paper, we contribute to the solution of the differential evasion game with multiple pursuers by building an exact strategy for the evader.
Hybrid AI-Analytical Modeling of Droplet Dynamics on Inclined Heterogeneous Surfaces
Authors: Demou Andreas D. , Savva Nikos
Published: April 2024

This work presents a novel approach for the study of the movement of droplets on inclined surfaces under the influence of gravity and chemical heterogeneities. The developed numerical methodology uses data-driven modeling to extend the applicability limits of an analytically derived reduced-order model for the contact line velocity. More specifically, while the reduced-order model is able to capture the effects of the chemical heterogeneities to a satisfactory degree, it does not account for gravity. To alleviate this shortcoming, datasets generated from direct numerical simulations are used to train a data-driven model for the contact line velocity, which is based on the Fourier neural operator and corrects the reduced-order model predictions to match the reference solutions. This hybrid surrogate model, which comprises of both analytical and data-driven components, is then integrated in time to simulate the droplet movement, offering a speedup of five orders of magnitude compared to direct numerical simulations. The performance of this hybrid model is quantified and assessed in different wetting scenarios, by considering various inclination angles and values for the Bond number, demonstrating the accuracy of the predictions as long as the adopted parameters lie within the ranges considered in the training dataset.
Cholestane-3β,5α,6β-triol Induces Multiple Cell Death in A549 Cells via ER Stress and Autophagy Activation
Authors: Chen Jiaxi , Zhang Jieping , Cai Lijuan , Guo Li , Cai Zhenyu , Han Hua , Zhang Wen
Published: April 2024

Cholestane-3β,5α,6β-triol (CT) and its analogues are abundant in natural sources and are reported to demonstrate cytotoxicity toward different kinds of tumor cells without a deep probe into their mechanism of action. CT is also one of the major metabolic oxysterols of cholesterol in mammals and is found to accumulate in various diseases. An extensive exploration of the biological roles of CT over the past few decades has established its identity as an apoptosis inducer. In this study, the effects of CT on A549 cell death were investigated through cell viability assays. RNA-sequencing analysis and western blot of CT-treated A549 cells revealed the role of CT in inducing endoplasmic reticulum (ER) stress response and enhancing autophagy flux, suggesting a putative mechanism of CT-induced cell-death activation involving reactive oxygen species (ROS)-mediated ER stress and autophagy. It is reported for the first time that the upregulation of autophagy induced by CT can serve as a cellular cytotoxicity response in accelerating CT-induced cell death in A549 cells. This research provides evidence for the effect of CT as an oxysterol in cell response to oxidative damage and allows for a deep understanding of cholesterol in its response in an oxidative stress environment that commonly occurs in the progression of various diseases.
Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
Authors: Campàs Mònica , Reverté Jaume , Tudó Àngels , Alkassar Mounira , Diogène Jorge , Sureda Francesc X.
Published: April 2024

Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC50 of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a Sphoeroides pachygaster specimen (TTX-free) when analysed at 10 mg/mL. The LOD achieved with this technique was 0.05 mg TTX equiv./kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv./kg. The APC system was applied to the analysis of extracts of a Lagocephalus sceleratus specimen, showing TTX contents that followed the trend of gonads > liver > skin > muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood.
Treatment Nonadherence among Multimorbid Chronic Disease Patients: Evidence from 3515 Subjects in Indonesia
Authors: Pradipta Ivan Surya , Aprilio Kevin , Ningsih Yozi Fiedya , Pratama Mochammad Andhika Aji , Alfian Sofa Dewi , Abdulah Rizky
Published: April 2024

Background and Objectives: Multimorbid patients require intensive treatment for their diseases. However, little research has been given to their treatment adherence as part of its management. This study aims to determine the prevalence and characteristics of chronic disease multimorbidity in Indonesia, alongside its treatment nonadherence. Materials and Methods: We conducted a cross-sectional study using the fifth Indonesian Family Life Survey database among adult subjects aged ≥ 15 years with multimorbidity. Our descriptive and multivariate analyses include sex, age, formal education, ethnicity, geographic residence, demographic residence, household size, insurance ownership, annual income, current self-perceived health status, missing active days, smoking behavior, and body mass index. Results: We identified 3515 multimorbid patients, constituting 30.8% prevalence across chronic disease patients. Hypertension was found to be a prevalent component of multimorbidity (61.2%), followed by digestive diseases (44.5%) and arthritis (30.3%). We identified that 36.4% of the subjects were nonadherent to their chronic disease treatment. Characteristics associated with nonadherence were found to be a good self-perception of health (aOR 1.79, 95% CI 1.54–2.08), active smoking behavior (aOR 1.51, 95% CI 1.14–1.99), no smoking behavior (aOR 1.44, 95% CI 1.08–1.90), missing seven active/productive days or less in the past month due to poor health (aOR 1.36, 95% CI 1.10–1.68), no insurance ownership (aOR 1.20, 95% CI 1.04–1.39), age of 15–65 years (aOR 1.25, 95% CI 1.01–1.55), income below IDR 40 million (aOR 1.23, 95% CI 1.04–1.46), and household size of 2–6 people (aOR 1.17, 95% CI 1.01–1.36). Conclusions: While the prevalence of multimorbidity in Indonesia is generally similar to that observed in previous studies, we have identified patient characteristics related to nonadherence. We suggest that patient’s nonadherence was primarily dictated by their self-perception of health and treatment complexity. With the longstanding issue of nonadherence, this study indicated the need to consider creating patient-tailored treatment programs in clinical practice to improve adherence by considering individual patients’ characteristics.
Maternal Diabetes Mellitus and Neonatal Outcomes in Bisha: A Retrospective Cohort Study
Authors: Alshomrany Abdullah , Miskeen Elhadi , Alfaifi Jaber , Alshamrani Hassan , Alshahrani Abdulmohsen
Published: April 2024

Background: Maternal diabetes mellitus (MDM) is associated with increased risks for adverse neonatal outcomes. However, the impact of MDM on neonatal outcomes in Bisha, a city in Saudi Arabia, is not well documented. This study aims to investigate the impact of MDM on neonatal outcomes in the Maternity and Children’s Hospital (MCH), Bisha, Saudi Arabia. Methods: A retrospective cohort study was conducted on 181 pregnant women with diabetes and their neonates who were diagnosed at the Maternity and Children’s Hospital (MCH), Bisha, Saudi Arabia, between 5 October 2020 and 5 November 2022. The primary outcome was a composite of adverse neonatal outcomes, including stillbirth, neonatal death, macrosomia, preterm birth, respiratory distress syndrome, hypoglycemia, and congenital anomalies. Logistic regression analyses were used to adjust for potential confounders. Results: The total sample size was 181. The average age of patients was 34 years (SD = 6.45). The majority of the patients were diagnosed with GDM, 147 (81.2%), and pre-GDM, 34 (18.8%). Neonates born to mothers with MDM had a higher risk of adverse neonatal outcomes compared to those born to mothers without MDM (adjusted odds ratio [aOR] = 1.46, 95% confidence interval [CI]: 1.25–1.70). The risks of macrosomia (aOR = 1.74, 95% CI: 1.38–2.19), LBW (aOR = 1.32, 95% CI: 1.06–1.66), and RDS (aOR = 1.57, 95% CI: 1.28–1.93) were significantly higher among neonates born to mothers with MDM. The types of DM were statistically significant in terms of their correlation with the following neonatal outcomes: hypoglycemia (p-value = 0.017), macrosomia (p-value = 0.050), and neonatal death (p-value = 0.017). Conclusions: MDM is associated with an increased risk of adverse neonatal outcomes in Bisha. The early identification and management of MDM may improve neonatal outcomes and reduce the burden of neonatal morbidity and mortality in this population.
Planning Mechanical Behavior of A356 Alloy Wheels by Using Distinct Heat Treatments
Authors: Tonetti Adriano L. , Osório Wislei R. , Bortolozo Ausdinir D. , Padilha Giovana S.
Published: April 2024

The aim of this investigation concerns evaluating the mechanical strength and microhardness values of A356 alloy samples in distinctive heat treatments, including those commonly applied to automotive wheels. It is recognized that A356 and Al-Si-based alloys exhibit considerable versatility across numerous industrial applications. The mechanical behavior obtained is intimately associated with different operational parameters (e.g., cooling rates, solution treatment, quenching, and artificial aging). In this study, a group of samples are quenched at 30, 60 and 80 °C. Another set is quenched and subsequently aged at three different temperatures, i.e., 180, 200, and 220 °C for 5 h, and mechanical responses are compared. Microstructural characterization, X-ray diffraction (XRD) analysis, tensile testing, and microhardness measurements are carried out. Using the Rietveld data and based on the modified Williamson–Hall method, the microstrains, crystallite size, and dislocation densities are calculated. Based on this, the resulting mechanical strengths from distinctive quenching and aging are understood. It was found that there exists a “quasi-optimal range” of operational parameters involving different A356 alloy treatments, which vary depending on the manufacturing route. Considering A356 alloy wheels, the planning of the powder coat treatment before or after T6 treating provides better mechanical properties and ductility.
Orientation Relationship of the Intergrowth Al13Fe3 and Al13Fe4 Intermetallics Determined by Single-Crystal X-ray Diffraction
Authors: Liu Yibo , Fan Changzeng , Xu Zhefeng , Fu Ruidong , Wen Bin , Zhang Lifeng
Published: April 2024

In the Al-Fe binary system, the Al13Fe3 phase as well as the Al13Fe4 phase has similar icosahedral building blocks like those appearing in quasicrystals. Therefore, it is of vital importance to clarify the formation process of these two phases. Coexistence of the Al13Fe3 and Al13Fe4 phases was discovered from the educts obtained with a nominal atomic ratio of Al/Fe of 9:2 by high-pressure sintering for the first time. Firstly, single crystal X-ray diffraction (SXRD) combined with a scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDX) measurement capabilities were adopted to determine the detailed crystal structures of both phases, which were sharply refined with regard to Al13Fe3 and Al13Fe4. Secondly, the orientation relationship between Al13Fe3 and Al13Fe4 was directly deduced from the SXRD datasets and the coexistence structure model was consequently constructed. Finally, seven pairs of parallel atomic planes and their unique orientation relations were determined from the reconstructed reciprocal space precession images. In addition, the real space structure model of the intergrowth crystal along with one kind of interfacial atomic structure were constructed from the determined orientation relations between two phases.
Synthesis of Sucrose-Mimicking Disaccharide by Intramolecular Aglycone Delivery
Authors: Sano Kanae , Ishiwata Akihiro , Takamori Hiroto , Kikuma Takashi , Tanaka Katsunori , Ito Yukishige , Takeda Yoichi
Published: April 2024

Rare sugars are known for their ability to suppress postprandial blood glucose levels. Therefore, oligosaccharides and disaccharides derived from rare sugars could potentially serve as functional sweeteners. A disaccharide [α-d-allopyranosyl-(1→2)-β-d-psicofuranoside] mimicking sucrose was synthesized from rare monosaccharides D-allose and D-psicose. Glycosylation using the intermolecular aglycon delivery (IAD) method was employed to selectively form 1,2-cis α-glycosidic linkages of the allopyranose residues. Moreover, β-selective psicofuranosylation was performed using a psicofuranosyl acceptor with 1,3,4,6-tetra-O-benzoyl groups. This is the first report on the synthesis of non-reducing disaccharides comprising only rare d-sugars by IAD using protected ketose as a unique acceptor; additionally, this approach is expected to be applicable to the synthesis of functional sweeteners.
Perennial Baki™ Bean Safety for Human Consumption: Evidence from an Analysis of Heavy Metals, Folate, Canavanine, Mycotoxins, Microorganisms and Pesticides
Authors: Craine Evan B. , Şakiroğlu Muhammet , Barriball Spencer , Peters Tessa E. , Schlautman Brandon
Published: April 2024

Global food production relies on annual grain crops. The reliability and productivity of these crops are threatened by adaptations to climate change and unsustainable rates of soil loss associated with their cultivation. Perennial grain crops, which do not require planting every year, have been proposed as a transformative solution to these challenges. Perennial grain crops typically rely on wild species as direct domesticates or as sources of perenniality in hybridization with annual grains. Onobrychis spp. (sainfoins) are a genus of perennial legumes domesticated as ancient forages. Baki™ bean is the tradename for pulses derived from sainfoins, with ongoing domestication underway to extend demonstrated benefits to sustainable agriculture. This study contributes to a growing body of evidence characterizing the nutritional quality of Baki™ bean. Through two studies, we investigated the safety of Baki™ bean for human consumption. We quantified heavy metals, folate, and canavanine for samples from commercial seed producers, and we quantified levels of mycotoxins, microorganisms, and pesticides in samples from a single year and seed producer, representing different varieties and production locations. The investigated analytes were not detectable or occurred at levels that do not pose a significant safety risk. Overall, this study supports the safety of Baki™ bean for human consumption as a novel pulse crop.
Artemisinin Confers Cytoprotection toward Hydrogen Peroxide-Induced Cell Apoptosis in Retinal Pigment Epithelial Cells in Correlation with the Increased Acetylation of Histone H4 at Lysine 8
Authors: Yang Chao , Ge Lijun , Yu Xiyong , Lazarovici Philip , Zheng Wenhua
Published: April 2024

Increased oxidative stress is one of the critical pathologies inducing age-related macular degeneration (AMD), characterized by retinal pigment epithelial (RPE) cell damage and death. The unbalanced acetylation and deacetylation of histones have been implicated in AMD pathogenesis or hydrogen peroxide (H2O2)-induced cell damage. Therefore, strategies aimed at controlling the balance between acetylation and deacetylation may effectively protect RPE cells from oxidative damage. Artemisinin is an antimalarial lactone drug derived from Artemisia annua, with antioxidant activity known to modulate histone acetylation in the brain, but its effect on the retina is unknown. In this study, we aimed to investigate whether Artemisinin exerts a cytoprotective effect on oxidative stress-induced apoptosis in RPE cells by regulating histone acetylation. We hypothesized that Artemisinin confers cytoprotection toward H2O2-induced apoptosis in RPE cells through this mechanism. In the present study, we found that Artemisinin at a sub-clinic dosage of 20 μM inhibited the H2O2-induced cell viability decrease and B-cell lymphoma 2 (Bcl-2) protein level decrease and attenuated the H2O2-induced decrease in the histone H4 lysine (Lys) 8 acetylation [Acetyl-H4 (Lys 8)] level in the retinal RPE cell line D407. As expected, histone deacetylase inhibitor Trichostatin A at the concentration of 250 nM increased the Acetyl-H4 (Lys 8) level in D407 cells and attenuated the H2O2-induced cell viability decrease and apoptosis. Similar findings were obtained using adult RPE (ARPE)19 cells, another human RPE cell line, and primary human RPE cell cultures. In conclusion, these results confirmed our hypothesis and indicated that Artemisinin attenuated H2O2-induced apoptosis in apparent correlation with the increase in the Acetyl-H4 (Lys 8) level, which is associated with gene transcription and cell survival. By modulating histone acetylation, Artemisinin may restore the balance between acetylation and deacetylation and enhance the resistance and survival of RPE cells under oxidative stress. Our study provides novel mechanistic insights into the effect of Artemisinin on histone acetylation and apoptosis in RPE cells and supports the potential application of Artemisinin in the prevention and/or treatment of AMD.
No Evidence Was Found for the Presence of Terreolides, Terreumols or Saponaceolides H-S in the Fruiting Bodies of Tricholoma terreum (Basidiomycota, Agaricales)
Authors: Clericuzio Marco , Serra Stefano , Vidari Giovanni
Published: April 2024

Two different collections of the gilled wild fungus Tricholoma terreum, collected in Italy, were subjected to phytochemical analysis. The fungal material was confidently identified by analysis of the ITS genomic sequences. Using both HR-LC-MS and NMR techniques, no evidence was found for the presence in the fruiting bodies of terreolides, terreumols or saponaceolides H-S, in striking contrast with the isolation of these terpenoids by Chinese authors from a mushroom collected in France and identified as T. terreum. The main cytotoxic terpenoid identified and isolated from the extracts of the specimens investigated in this work was the C30 derivative saponaceolide B, which had been previously isolated from T. saponaceum and other T. terreum collections. Although saponaceolide B is a rather labile molecule, easily degradable by heat or in acidic conditions, our study indicated that none of the extraction protocols used produced saponaceolide H-S or terreolide/terreumol derivatives, thus excluding the possibility that the latter compounds could be extraction artifacts. Considered together, these findings point to the need for the unambiguous identification of mushroom species belonging to the complex genus Tricholoma, characterized by high variability in the composition of metabolites. Moreover, based on our data, T. terreum must be considered an edible mushroom.
Smartfeeding: A Dynamic Strategy to Increase Nutritional Efficiency in Critically Ill Patients—Positioning Document of the Metabolism and Nutrition Working Group and the Early Mobilization Working Group of the Catalan Society of Intensive and Critical Care Medicine (SOCMiC)
Authors: Yébenes Juan Carlos , Bordeje-Laguna Maria Luisa , Lopez-Delgado Juan Carlos , Lorencio-Cardenas Carol , Martinez De Lagran Zurbano Itziar , Navas-Moya Elisabeth , Servia-Goixart Lluis
Published: April 2024

Adequate medical nutrition therapy for critically ill patients has an impact on their prognoses. However, it requires an individualized approach that takes into account the activity (phases of metabolic stress) and particularity of these patients. We propose a comprehensive strategy considering the patients’ nutritional status and the set of modifiable circumstances in these patients, in order to optimize/support nutritional efficiency: (1) A detailed anamnesis and an adequate initial nutritional assessment must be performed in order to implement medical nutrition therapy that is in line with the needs and characteristics of each patient. Furthermore, risks associated with refeeding syndrome, nutritrauma or gastrointestinal dysfunction must be considered and prevented. (2) A safe transition between nutrition therapy routes and between health care units will greatly contribute to recovery. The main objective is to preserve lean mass in critically ill patients, considering metabolic factors, adequate protein intake and muscle stimulation. (3) Continuous monitoring is required for the successful implementation of any health strategy. We lack precise tools for calculating nutritional efficiency in critically ill patients, therefore thorough monitoring of the process is essential. (4) The medical nutrition approach in critically ill patients is multidisciplinary and requires the participation of the entire team involved. A comprehensive strategy such as this can make a significant difference in the functional recovery of critically ill patients, but leaders must be identified to promote training, evaluation, analysis and feedback as essential components of its implementation, and to coordinate this process with the recognition of hospital management.
Breaking Conventional Eating Habits: Perception and Acceptance of 3D-Printed Food among Taiwanese University Students
Authors: Chang Min-Yen , Hsia Wei-Jiun , Chen Han-Shen
Published: April 2024

Considering the prevalent strain on environmental resources imparted by existing food systems, prioritizing environmental sustainability is an imperative course of action. Subsequently, the shift towards sustainable production and consumption patterns engenders an escalating demand for environmentally conscious food systems. Thus, 3D-printed food technology surfaces are a promising solution noted for their efficacy in curtailing food waste, bolstering environmental sustainability, and imparting innovative strategies to the food supply chain. Herein, we amalgamate the theory of planned behavior (TPB) framework with several variables, namely ‘sensory appeal’, ‘food neophobia’, ‘perceived health risk’, and ‘environmental friendliness’ to probe the behavioral intentions of Taiwanese university students’ perceptions about 3D-printed food. Employing the snowball sampling method, 370 questionnaires were disseminated, out of which 319, constituting an effective retrieval rate of 86.2%, were deemed valid. Statistical analysis produced intriguing findings. Consumers’ inclination to purchase 3D-printed food is substantially determined by their attitudes, subjective norms, sensory appeal, food neophobia, perceived health risks, and environmental friendliness. Contrary to our initial hypothesis, perceived behavioral control did not exhibit a significant impact on consumers’ propensity to purchase 3D-printed food. Therefore, businesses should focus on magnifying the sensory appeal of 3D-printed food, coupled with precise nutritional labeling, to bolster consumer interest, enhance acceptance, and augment behavioral intentions. This study sheds light on the potential for the development of 3D-printed food in Taiwan, providing an indispensable reference for future endeavors in Taiwan’s 3D-printed food industry.
Exercise Prescription for Postprandial Glycemic Management
Authors: Bellini Alessio , Scotto di Palumbo Alessandro , Nicolò Andrea , Bazzucchi Ilenia , Sacchetti Massimo
Published: April 2024

The detrimental impacts of postprandial hyperglycemia on health are a critical concern, and exercise is recognized a pivotal tool in enhancing glycemic control after a meal. However, current exercise recommendations for managing postprandial glucose levels remain fairly broad and require deeper clarification. This review examines the existing literature aiming to offer a comprehensive guide for exercise prescription to optimize postprandial glycemic management. Specifically, it considers various exercise parameters (i.e., exercise timing, type, intensity, volume, pattern) for crafting exercise prescriptions. Findings predominantly indicate that moderate-intensity exercise initiated shortly after meals may substantially improve glucose response to a meal in healthy individuals and those with type 2 diabetes. Moreover, incorporating short activity breaks throughout the exercise session may provide additional benefits for reducing glucose response.
Eugenol Inhibits Neutrophils Myeloperoxidase In Vitro and Attenuates LPS-Induced Lung Inflammation in Mice
Authors: Chniguir Amina , Saguem Mohamed Hedi , Dang Pham My-Chan , El-Benna Jamel , Bachoual Rafik
Published: April 2024

Eugenol (Eug) is a polyphenol extracted from the essential oil of Syzygium aromaticum (L.) Merr. and Perry (Myrtaceae). The health benefits of eugenol in human diseases were proved in several studies. This work aims to evaluate the effect of eugenol on lung inflammatory disorders. For this, using human neutrophils, the antioxidant activity of eugenol was investigated in vitro. Furthermore, a model of LPS-induced lung injury in mice was used to study the anti-inflammatory effect of eugenol in vivo. Results showed that eugenol inhibits luminol-amplified chemiluminescence of resting neutrophils and after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLF) peptide or phorbol myristate acetate (PMA). This effect was dose dependent and was significant from a low concentration of 0.1 µg/mL. Furthermore, eugenol inhibited myeloperoxidase (MPO) activity without affecting its degranulation. Eugenol has no scavenging effect on hydrogen peroxide (H2O2) and superoxide anion (O2−). Pretreatment of mice with eugenol prior to the administration of intra-tracheal LPS significantly reduced neutrophil accumulation in the bronchoalveolar lavage fluid (BALF) and decreased total proteins concentration. Moreover, eugenol clearly inhibited the activity of matrix metalloproteinases MMP-2 (21%) and MMP-9 (28%), stimulated by LPS administration. These results suggest that the anti-inflammatory effect of eugenol against the LPS-induced lung inflammation could be exerted via inhibiting myeloperoxidase and metalloproteinases activity. Thus, eugenol could be a promising molecule for the treatment of lung inflammatory diseases.
Transcriptome Analysis Reveals Key Genes Involved in Trichome Formation in Pepper (Capsicum annuum L.)
Authors: Shen Yiyu , Mao Lianzhen , Zhou Yao , Sun Ying , Lv Junheng , Deng Minghua , Liu Zhoubin , Yang Bozhi
Published: April 2024

Trichomes are specialized organs located in the plant epidermis that play important defense roles against biotic and abiotic stresses. However, the mechanisms regulating the development of pepper epidermal trichomes and the related regulatory genes at the molecular level are not clear. Therefore, we performed transcriptome analyses of A114 (less trichome) and A115 (more trichome) to dig deeper into the genes involved in the regulatory mechanisms of epidermal trichome development in peppers. In this study, the epidermal trichome density of A115 was found to be higher by phenotypic observation and was highest in the leaves at the flowering stage. A total of 39,261 genes were quantified by RNA-Seq, including 11,939 genes not annotated in the previous genome analysis and 18,833 differentially expressed genes. Based on KEGG functional enrichment, it was found that DEGs were mainly concentrated in three pathways: plant–pathogen interaction, MAPK signaling pathway-plant, and plant hormone signal transduction. We further screened the DEGs associated with the development of epidermal trichomes in peppers, and the expression of the plant signaling genes GID1B-like (Capana03g003488) and PR-6 (Capana09g001847), the transcription factors MYB108 (Capana05g002225) and ABR1-like (Capana04g001261), and the plant resistance genes PGIP-like (Capana09g002077) and At5g49770 (Capana08g001721) in the DEGs were higher at A115 compared to A114, and were highly expressed in leaves at the flowering stage. In addition, based on the WGCNA results and the establishment of co-expression networks showed that the above genes were highly positively correlated with each other. The transcriptomic data and analysis of this study provide a basis for the study of the regulatory mechanisms of pepper epidermal trichomes.
Response of Phyllosphere and Rhizosphere Microbial Communities to Salt Stress of Tamarix chinensis
Authors: Qu Xuan , Pan Yaqing , Wang Peiqin , Ran Lele , Qin Guifei , Li Qunfang , Kang Peng
Published: April 2024

As carriers of direct contact between plants and the atmospheric environment, the microbiomes of phyllosphere microorganisms are increasingly recognized as an important area of study. Salt secretion triggered by salt-secreting halophytes elicits changes in the community structure and functions of phyllosphere microorganisms, and often provides positive feedback to the individual plant/community environment. In this study, the contents of Na+ and K+ in the rhizosphere, plant and phyllosphere of Tamarix chinensis were increased under 200 mmol/L NaCl stress. The increase in electrical conductivity, Na+ and K+ in the phyllosphere not only decreased the diversity of bacterial and fungal communities, but also decreased the relative abundance of Actinobacteriota and Basidiomycota. Influenced by electrical conductivity and Na+, the bacteria–fungus co-occurrence network under salt stress has higher complexity. Changes in the structure of the phyllosphere microbial community further resulted in a significant increase in the relative abundance of the bacterial energy source and fungal pathotrophic groups. The relative abundance of Actinobacteriota and Acidobacteriota in rhizosphere showed a decreasing trend under salt stress, while the complexity of the rhizosphere co-occurrence network was higher than that of the control. In addition, the relative abundances of functional groups of rhizosphere bacteria in the carbon cycle and phosphorus cycle increased significantly under stress, and were significantly correlated with electrical conductivity and Na+. This study investigated the effects of salinity on the structure and physicochemical properties of phyllosphere and rhizosphere microbial communities of halophytes, and highlights the role of phyllosphere microbes as ecological indicators in plant responses to stressful environments.
Physiological and Transcriptomic Responses of Bok Choy to Heat Stress
Authors: Dong Cuina , Peng Xixuan , Yang Xiaona , Wang Chenggang , Yuan Lingyun , Chen Guohu , Tang Xiaoyan , Wang Wenjie , Wu Jianqiang , Zhu Shidong , Huang Xingxue , Zhang Jinlong , Hou Jinfeng
Published: April 2024

High temperatures have adverse effects on the yield and quality of vegetables. Bok choy, a popular vegetable, shows varying resistance to heat. However, the mechanism underlying the thermotolerance of bok choy remains unclear. In this study, 26 bok choy varieties were identified in screening as being heat-resistant at the seedling stage; at 43 °C, it was possible to observe obvious heat damage in different bok choy varieties. The physiological and biochemical reactions of a heat-tolerant cultivar, Jinmei (J7), and a heat-sensitive cultivar, Sanyueman (S16), were analyzed in terms of the growth index, peroxide, and photosynthetic parameters. The results show that Jinmei has lower relative conductivity, lower peroxide content, and higher total antioxidant capacity after heat stress. We performed transcriptome analysis of the two bok choy varieties under heat stress and normal temperatures. Under heat stress, some key genes involved in sulfur metabolism, glutathione metabolism, and the ribosome pathway were found to be significantly upregulated in the heat-tolerant cultivar. The key genes of each pathway were screened according to their fold-change values. In terms of sulfur metabolism, genes related to protease activity were significantly upregulated. Glutathione synthetase (GSH2) in the glutathione metabolism pathway and the L3e, L23, and S19 genes in the ribosomal pathway were significantly upregulated in heat-stressed cultivars. These results suggest that the total antioxidant capacity and heat injury repair capacity are higher in Jinmei than in the heat-sensitive variety, which might be related to the specific upregulation of genes in certain metabolic pathways after heat stress.
Exogenous Spermidine Alleviated Low-Temperature Damage by Affecting Polyamine Metabolism and Antioxidant Levels in Apples
Authors: He Meiqi , Zhou Jia , Lyu Deguo , Xu Gongxun , Qin Sijun
Published: April 2024

Low-temperature stress significantly limits the growth, development, and geographical distribution of apple cultivation. Spermidine (Spd), a known plant growth regulator, plays a vital role in the plant’s response to abiotic stress. Yet, the mechanisms by which exogenous Spd enhances cold resistance in apples remain poorly understood. Therefore, the present study analyzed the effects of exogenous Spd on antioxidant enzyme activity, polyamine metabolism, and related gene expression levels of 1-year-old apple branches under low-temperature stress. Treatment with exogenous Spd was found to stabilize branch tissue biofilms and significantly reduce the levels of reactive oxygen species by elevating proline content and boosting the activity of antioxidants such as superoxide dismutase. It also upregulated the activities of arginine decarboxylase, S-adenosylmethionine decarboxylase, and spermidine synthase and the expression levels of MdADC1, MdSAMDC1, and MdSPDS1 under low-temperature stress and led to the accumulation of large amounts of Spd and spermine. Moreover, compared with the 2 mmol·L−1 Spd treatment, the 1 mmol·L−1 Spd treatment increased the expression levels of cold-responsive genes MdCBF1/2/3, MdCOR47, and MdKIN1, significantly. The findings suggest that exogenous Spd can enhance cold resistance in apple branches significantly. This enhancement is achieved by modulating polyamine metabolism and improving antioxidant defense mechanisms, which could be exploited to improve apple cultivation under cold stress conditions.
Field Work in Papua New Guinea Documents Seven New Records of a Hemiepiphytic Habit in Ferns
Authors: Sundue Michael , Maraia Heveakore
Published: April 2024

Hemiepiphytes have captured the attention of biologists since they seemingly hold clues to the evolution of epiphytes themselves. Hemiepiphytes are known to occur sporadically in the leptosporangiate ferns, but our understanding of their evolution remains limited by the relatively small number of detailed observations. This study adds to our knowledge by documenting seven species previously assumed to be holoepiphytes. This finding was based on fieldwork conducted in the Baining Mountains of Papua New Guinea that resulted in 319 collections representing 206 species. Approximately 3% of these species were hemiepiphytes: Asplenium acrobryum, A. amboinense, A. scandens, A. scolpendropsis, Crepidomanes aphlebioides, Leptochilus macrophyllus, and Sphaerostephanos scandens. All started growth as low-trunk epiphytes, and later, as larger climbing plants, exhibited strongly dimorphic roots consisting of short clasping ones that affixed the rhizome to the trunks and long feeding roots that entered the soil. Most of the seven hemiepiphyte species that we found exhibited distichous phyllotaxy and dorsiventrally flattened rhizomes, suggesting morphological convergence associated with this habit in four families. These new records suggest that large hemiepiphytic clades occur in Asplenium and Leptochilus. Our observations expand the geographic and taxonomic breadth of hemiepiphytic ferns, provide a baseline estimate of their diversity within a tropical flora, and offer morphological and phylogenetic clues to uncover additional records.
Effect of Elicitor Treatments on Quality Attributes in Blueberry: Implications of Cultivar and Environmental Conditions
Authors: Cola Gabriele , Cavenago Beatrice , Gardana Claudio Sebastiano , Spinardi Anna
Published: April 2024

Elicitors of plant defence responses can trigger defence mechanisms that are able to protect plant tissues from biotic or abiotic stresses. Since one defence response involves the activation of secondary metabolites’ biosynthesis, the purpose of this study was to evaluate the effect of chitosan and melatonin pre-harvest treatments on the quality and the nutritional parameters of the fruits of blueberry (Vaccinium corymbosum L.). Across the two years of experiment, three different cultivars (cv.s. ‘Cosmopolitan’, ‘Hortblue Poppins’ and ‘Legacy’) were treated with 1% chitosan or 100 µM melatonin every two weeks during the ripening season and ripe fruits were progressively harvested and analysed. The treatment with both elicitors had only slight effects on dry matter, soluble solids content, titratable acidity and pH, with a cultivar-dependent response. On the other hand, elicitors significantly affected the levels of phenylpropanoid and antioxidant compounds in all cvs. in both years, with a higher accumulation of total anthocyanins and phenolics and the enhancement of the antioxidant capacity, with positive effects on the nutraceutical quality of fruits. The anthocyanin profile in terms of both absolute concentrations and the relative proportion of single anthocyanins was affected by both harvest year and cv., highlighting the role of the genetic background in the plant response to environmental conditions (with particular reference to summer heat stress) and to elicitor treatments.
Evaluation of the Effect of a Spray Coating Applied on Open-Air-Stored Woodchips
Authors: Picchi Gianni , Nati Carla , Brilli Lorenzo , Cinotti Alessandro
Published: April 2024

The present study aimed at testing the benefits of protecting woodchips with an acrylic crusting product developed for the coal energy industry. In the test carried out, four conical wood chips piles were built, two consisting of fresh biomass, the other two of dry wood chips. A fourth larger pile was built as a reference. One dry and one fresh pile were superficially treated with 25 kg of protective acrylic solution diluted in 250 L of water, providing an average application of coating agent of approximately 85 g m−2, while the other two worked as controls. To monitor the piles’ temperature variation, thermal sensors were placed in the inner part of the five piles during their construction. Moisture content (MC) and dry matter (DM) variations in woodchip piles were recorded. The piles treated with the coating agent did not show any significant differences with the untreated piles: in wet material, the protective film slightly reduced the moisture dispersal from the pile from evaporation rather than limiting water intake from rain; in dry material, this confirms the inability of the coating agent to limit water intake from rainfall.
Designing Digital Twins of Robots Using Simscape Multibody
Authors: Boschetti Giovanni , Sinico Teresa
Published: April 2024

Digital twins of industrial and collaborative robots are widely used to evaluate and predict the behavior of manipulators under different control strategies. However, these digital twins often employ simplified mathematical models that do not fully describe their dynamics. In this paper, we present the design of a high-fidelity digital twin of a six degrees-of-freedom articulated robot using Simscape Multibody, a Matlab toolbox that allows the design of robotic manipulators in a rather intuitive and user-friendly manner. This robot digital twin includes joint friction, transmission gears, and electric actuators dynamics. After assessing the dynamic accuracy of the Simscape model, we used it to test a computed torque control scheme, proving that this model can be reliably used in simulations with different aims, such as validating control schemes, evaluating collaborative functions or minimizing power consumption.
Exploring the InSAR Deformation Series Using Unsupervised Learning in a Built Environment
Authors: Yang Mengshi , Li Menghua , Huang Cheng , Zhang Ruisi , Liu Rui
Published: April 2024

As a city undergoes large-scale construction and expansion, there is an urgent need to monitor the stability of the ground and infrastructure. The time-series InSAR technique is an effective tool for measuring surface displacements. However, interpreting these displacements in a built environment, where observed displacements consist of mixed signals, poses a challenge. This study uses principal component analysis (PCA) and the k-means clustering method for exploring deformation series within an unsupervised learning context. The PCA method extracts the dominant components in deformation series, whereas the clustering method identifies similar deformation series. This method was tested on Kunming City (KMC) using C-band Sentinel-1, X-band TerraSAR-X, and L-band ALOS-2 PALSAR-2 data acquired between 2017 to 2022. The experiment demonstrated that the suggested unsupervised learning approach can group PS points with similar kinematic characteristics. Five types of deformation kinematic characteristics were discovered in the three SAR datasets: upward, slight upward, stability, slight downward, and downward. According to the results, less than 20% of points exhibit significant motion trends, whereas 50% show small velocity values but still demonstrate movement trends. The remaining 30% are relatively stable. Similar clustering results were obtained from the three datasets using unsupervised methods, highlighting the effectiveness of identifying spatial–temporal patterns over the study area. Moreover, It was found that clustering based on kinematic characteristics enhances the interpretation of InSAR deformation, particularly for points with small deformation velocities. Finally, the significance of PCA decomposition in interpreting InSAR deformation was discussed, as it can better represent series with noise, enabling their accurate identification.
Joint Implementation Method for Clutter Suppression and Coherent Maneuvering Target Detection Based on Sub-Aperture Processing with Airborne Bistatic Radar
Authors: Sun Zhi , Jiang Xingtao , Zhang Haonan , Deng Jiangyun , Xiao Zihao , Cheng Chen , Li Xiaolong , Cui Guolong
Published: April 2024

An airborne bistatic radar working in downward-looking mode confronts two major challenges for low-altitude target detection. One is range cell migration (RCM) and Doppler migration (DM) resulting from the relative motion of the radar and target. The other is the non-stationarity characteristic of clutter due to the radar configuration. To solve these problems, this paper proposes a joint implementation method based on sub-aperture processing to achieve clutter suppression and coherent maneuvering target detection. Specifically, clutter Doppler compensation and sliding window processing are carried out to realize sub-aperture space–time processing, removing the clutter non-stationarity resulting from the bistatic geometric configuration. Thus, the output matrix of clutter suppression in the sub-aperture could be obtained. Then, the elements with the same phase of this matrix are superimposed and rearranged to achieve the reconstructed 2-D range-pluse echo matrix. Next, the aperture division with respect to slow time is conducted and the RCM correction based on modified location rotation transform (MLRT) and coherent integration (CI) are realized within each sub-aperture. Finally, the matched filtering process (MFP) is applied to compensate for the RCM/DM among different sub-apertures to coherently integrate the maneuvering target energy of all sub-apertures. The simulation and measured data processing results prove the validity of the proposed method.
A Fast IAA–Based SR–STAP Method for Airborne Radar
Authors: Zhang Shuguang , Wang Tong , Liu Cheng , Ren Bing
Published: April 2024

Space–time adaptive processing (STAP) is an effective technology in clutter suppression and moving target detection for airborne radar. When working in the heterogeneous environment, the number of training samples that satisfy independent and identically distributed (IID) conditions is insufficient, making it difficult to ensure the estimation accuracy of the clutter plus noise covariance matrix for traditional STAP methods. Sparse recovery–based STAP (SR–STAP) methods have received widespread attention in the past few years. The accurate estimation of the clutter plus noise covariance matrix can be achieved using only a few training samples. The iterative adaptive approach (IAA) can quickly and accurately estimate the power spectrum, but applying this method directly to the STAP method cannot produce good performance. In this paper, a fast IAA–based SR–STAP method is proposed. Based on the weighted l1 problem, the IAA spectrum is used as a weighted term to obtain a good approximation. In order to obtain an analytical solution, we use the weighted l2 norm to approximate the weighted l1 norm without loss of performance. Compared with the IAA–STAP method, the proposed method is more robust to errors. Moreover, the proposed method has a fast computational speed. The effectiveness of the proposed method is demonstrated by simulations.