bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Resistance of Aeromonas salmonicida Isolated From Common carp (Cyprinus carpio) Fishes in Erbil City/ Iraq
Authors: Et al. Mustafa
Published: September 2019

Aeromonas salmonicida is a fish pathogen and recognized to cause a variety of diseases in humans. There are a few information about A.salmonicida in Iraq and there is no any previous molecular study on it. During the period of December 2017 to May 2018; Sixteen isolates of the A. salmonicida were isolated and identified from 300 common carp (Cyprinus carpio) fishes stomach in aquarium of Erbil city/ Iraq by using manual, automated Vitek 2 compact system, and confirmed by PCR using gene TonB-dependent siderophore (364bp). Antimicrobial susceptibility was determined by disk diffusion method and the results found that all isolates 100% susceptible to imipenem, 100% resistant to nalidixic acid and variable resistance to other studied antibiotics. The antibacterial effect of aqueous and alcohol extracts of Eminium spiculatum was studied by MIC and Molecular studies. The results found that aqueous and alcohol extracts of E. spiculatum have inhibitory effect and the MIC were 1400, 1800 µg/ml respectively. The sub MIC was used for both plant extracts, in plasmid profile the number of plasmid DNA was differ when treated with plant extracts. The inhibitory effect of the plant extracts against total proteins was studied by SDS- polyacrylamide gel electrophoresis and the results showed that there were variations in the protein bands in studied isolates and induction of new bands after treating with plant extracts. It was concluded that the leaves extracts of E.spiculatum could be used as antimicrobial for treatment of A. salmonicida infections and the results were confirmed by molecular studies.
Investigating the Histological Changes in Heart, Lung, Liver and Kidney of Male Albino Mice Treated with Ivabradine
Authors: Khaleel Hadeel Kamil
Published: September 2019

The present study aimed to investigate the histological changes of heart, lung, liver and kidney which caused by different concentrations (10, 20 and 40 mg/kg) of Ivabradine. Results of the study revealed some histological changes represented by aggregation of the lymphocytes around respiratory bronchioles of the lung. In the liver, the drug caused hepatocyte necrosis and infiltration of the lymphocytes. In Kidney, there are no histopathological modifications in the tissue after the animals treated with 10 mg\kg of Ivabradine. When the animals treated with Ivabradine drug at 20mg/kg of bw, dose showed vascular congestion between myocardial fibers of heart. Emphysematous changes of the alveoli and infiltration of lymphocytes around respiratory bronchioles of lung. In the liver there were dilated blood sinusoids. Also, there are vascular congestion and congestion of capillaries in the glomerular of kidney. Male mice treated with Ivabradine drug at 40 mg/kg of bw cause increase spaces between myocardial fibers, cardiac atrophy and myocardial degeneration in the heart. In addition, there are infiltration of lymphocytes around respiratory bronchioles, pulmonary congestion and emphysematous changes of the alveoli in lung. In the liver, the drug cause amyloid deposition and degeneration of hepatocytes. Furthermore, the drug caused vascular congestion in the kidney. Conclusion: From the current study, we conclude that the different concentrations of Ivabradine caused tissue changes in the heart, lung, liver and kidneys. The study should continue using different drugs and concentrations.
Geometry, and Normal Modes of Vibration (3N-6) for Di and Tetra-Rings Layer (6, 0) Linear (Zigzag) SWCNTs; A DFT Treatment
Authors: Et al. Kubba
Published: September 2019

Density Functional Theory (DFT) method of the type (B3LYP) and a Gaussian basis set (6-311G) were applied for calculating the vibration frequencies and absorption intensities for normal coordinates (3N-6) at the equilibrium geometry of the Di and Tetra-rings layer (6, 0) zigzag single wall carbon nanotubes (SWCNTs) by using Gaussian-09 program. Both were found to have the same symmetry of D6d point group with C--C bond alternation in all tube rings (for axial bonds, which are the vertical C--Ca bonds in rings layer and for circumferential bonds C—Cc in the outer and mid rings bonds). Assignments of the modes of vibration IR active and inactive vibration frequencies (symmetric and asymmetric modes) based on the image modes applied by the Gaussian 09 display. The whole relations for the vibration modes were also done including nCH stretching, nC--C stretching, δCH, δring (δC--C--C) deformation in plane of the molecule) and gCH, gring (gC--C--C) deformation out of plane of the molecule. The assignment also included modes of puckering, breathing and clock-anticlockwise bending vibrations.
Kinetic Studies of Na+/K+-ATPase in Tissue Aerobic Thyroid Patients
Authors: Et al. Al Samurai
Published: September 2019

Na+/K+-ATPase is a prevalent enzyme that maintains the Na+ and K+ gradients across the cell membrane by transporting three Na+ out and two K+ into the cell, the aim of this study  is to provide detailed mechanistic insights, potentially with important effects on physiological regulation of active Na and K transport in tissues of Aerobic Thyroid Patient. Thyroid tissues were obtained from a 35 year old patients, the operation was carried out at the Al-Hadi Specialist Hospital in Samarra city, the sample was stored at -20ºC until used. The purification protocol included Salt Precipitation, Ion Exchange Chromatography, Gel Filtration and Electrophoresis, a spectrophotometric method was   used to determine the enzyme activity. kinetic parameters  was also  obtained  for the enzyme. Partial purification of Na+/K+-ATPase revealed two isoenzymes (I ,II). The purity of separated isoenzymes were proved by SDS-PAGE electrophoresis. The kinetic characteristics of Na+/K+-ATPase showed that optimum substrate concentration about 1.5mM,  Km 1.052mM,  and Vmax 6.062, optimum temperature was 37 ºC, optimum pH  7.4 and optimum  time  in 25 min. Na+/K+-ATPase purified from Thyroid tissue  has distinct kinetic characteristic that reflects the importance of      intracellular regulation of specific Na+/K+-ATPase pump which gives cells the ability to precisely coordinate to their physiological requirements .
A Molecular and Biochemical Study for Cholesteryl Ester Transfer Protein (CETP) Taq1B in Iraqi Patients with Hyperlipidemia
Authors: Et al. Alkhafajy
Published: September 2019

Cholesteryl ester transfer protein gene contains some single nucleotide polymorphisms, which have been associated with serum high-density lipoprotein concentration and other lipoproteins. This study is done for determining of cholesteryl ester transfer protein polymorphism and evaluate its effect on serum lipid profile concentrations in some hyperlipidemic patients compared with healthy subjects in Salah Al-din governorate-Iraq. Blood samples were taken from (90) patients suffering from hyperlipidemia, and (70) samples that were apparently healthy controls.  Serum lipid concentrations were measured by enzymatic assays. The polymorphism was genotyped using polymerase chain reaction restriction fragment length polymorphism analysis.  The results showed that there was a significant decrease (P<0.05) in the frequency B2 allele, and B1B2, B2B2 genotype, and a significant increase (P<0.05) in the frequency B1 allele, and B1B1 genotype between patients and controls groups. There was a non-significant decrease in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, and non-significant increase in levels of triglycerides in individuals with the B1B1 genotype than in the B1B2 and B2B2 genotype. However, high density lipoproteins showed a significant decrease (P<0.001) between individuals with the B1B1 genotype and B2B2 genotype. Also, there was a non-significant difference in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, in individuals with the B1B2 genotype when compared with that of the B2B2 genotype.
Effect of pregnancy induced hypertension on adverse perinatal outcomes in Tigray Regional State, Ethiopia: a prospective cohort study
Authors: Berhe Abadi Kidanemariam , ILESANMI Abiodun O. , AIMAKHU Christopher O. , Mulugeta Afework
Published: September 2019

Background: Pregnancy induced hypertension is the development of new hypertension with or without proteinuria after 20 weeks of pregnancy. The prevalence of pregnancy induced hypertension in Ethiopia ranges from 2.2 to 18.3%. However, little is known about the adverse perinatal outcomes of pregnancy induced hypertension in Tigray Regional State, Ethiopia.Therefore the objective of this study was to assess effect of pregnancy induced hypertension on adverse perinatal outcomes in Tigray Regional State, Ethiopia. Methods: a prospective cohort study was conducted on a total sample of 782 pregnant women attending antenatal care in hospitals of Tigray regional state, Ethiopia. Data were collected using interviewer-administered questionnaire and review of their medical records from February 1, 2018, to February 30, 2019. Data analysis was performed using Stata version 14.0. Relative risk with 95% CI for the association between pregnancy induced hypertension and perinatal outcomes were assessed using Poisson regression analysis. Results: In this study the overall incidence of adverse perinatal outcome was higher among women with pregnancy induced hypertension than normotensive women ( 66.4% vs 22.2%). After adjusted for confounders women with pregnancy induced hypertension were born babies with a higher risk of low birth weight (adjusted RR(95%CI)= 5.1(3.4,7.8)), birth asphyxia (aRR=2.6(1.9,3.8)), small for gestational age (aRR=3.3(2.3,4.6)), preterm delivery (aRR=5.2(3.4,7.9)), admission to neonatal intensive care unit (aRR=5.1(3.1,8.4)) and with a higher risk of perinatal death (aRR=3.6(1.8,7.4)) compared to normotensive pregnant women Conclusions: Higher incidences of adverse perinatal outcomes were occurred among women pregnancy induced hypertension in Tigray regional state, Ethiopia. Hence health care providers should strengthen prevention, early diagnosis and prompt management of pregnancy induced hypertension to reduce adverse perinatal outcomes of pregnancy induced hypertension. Keywords : Adverse perinatal outcomes, pregnancy induced hypertension, Tigray, Ethiopia
Genome-wide association mapping of Sclerotinia sclerotiorum resistance in soybean using whole-genome resequencing data
Authors: Boudhrioua Chiheb , Bastien Maxime , Torkamaneh Davoud , Belzile François
Published: September 2019

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in soybean. Although many papers have reported different loci contributing to partial resistance, few of these were proved to reproduce the same phenotypic impact in different populations. In this study, we identified a major quantitative trait loci (QTL) associated with resistance to SSR progression on the main stem by using a genome-wide association mapping (GWAM). A population of 127 soybean accessions was genotyped with 1.5M SNPs derived from genotyping-by-sequencing (GBS) and whole-genome sequencing (WGS) ensuring an extensive genome coverage and phenotyped for SSR resistance. SNP-trait association led to discovery of a new QTL on chromosome 1 (Gm01) where resistant lines had shorter lesions on the stem by 29 mm. The impact of this QTL was even more significant in the descendants of a cross between two lines carrying contrasted alleles for Gm01. Individuals carrying the resistance allele developed lesions almost 50% shorter than those bearing the sensitivity allele. These results suggest that this region harbors a promising resistance QTL to SSR that can be used in soybean breeding program.
Shock wave-enhanced emission photoacoustic streaming versus photon-induced photoacoustic streaming modes for clearing root canal bacteria using erbium-doped yttrium aluminum garnet lasers: An in vitro study
Authors: Wang Xiao-Na , Shi Jing
Published: September 2019

Background : The root canal cleaning efficiency of the photon-induced photoacoustic streaming (PIPS) mode in erbium-doped yttrium aluminum garnet (Er:YAG) lasers is controversial. Shock wave-enhanced emission photoacoustic streaming (SWEEPS), a novel mode in Er:YAG lasers, creates a strong shock wave in a narrow container. We accordingly hypothesized that it may effectively eliminate bacteria from narrow root canals. The present study aimed to compare the bacterial clearance efficiency between the SWEEPS and PIPS modes for Er:YAG lasers designed for root canal treatment. Methods: The distal and palatal roots of 130 freshly extracted human molars were used. The smear layer was first removed by conventional root canal preparation. All samples were then sterilized in an autoclave. The samples were subsequently exposed to Enterococcus faecalis and incubated for 4 weeks to establish E. faecalis infection models. The models were divided into three groups according to the irrigation method (n = 40 per group): 3% sodium hypochlorite (NaOCl) activated using the SWEEPS Er:YAG laser mode, 3% NaOCl activated using the PIPS Er:YAG laser mode, and 3% NaOCl without activation. Results: The bacterial clearance efficiency, based on the colony-forming unit count, was significantly higher in the SWEEPS group than in the PIPS and NaOCl groups. Moreover, scanning electron microscopy after irrigation revealed that the SWEEPS group had the least number of bacteria. Conclusions: The SWEEPS mode may be more effective than the PIPS mode for eradicating E. faecalis from root canals.
Real-world data of eribulin in advanced and pretreated HER2-negative breast cancer in a Spanish comprehensive cancer center
Authors: Sirvén Milana Bergamino , Fernández-Ortega Adela , Stradella Agostina , Morilla Idoia , Falo Catalina , Vázquez Silvia , Castany Roser , Villanueva Rafael , Recalde Sabela , Pérez Valentí Navarro , Gil-Gil Miguel , Pernas Sonia
Published: September 2019

Background: Eribulin improves survival in pre-treated HER2-negative advanced breast cancer (ABC). However, limited data exist on co-morbidities and central nervous system (CNS) efficacy. The purpose of this study was to review eribulin’s efficacy and safety in everyday clinical practice with special focus on age, body mass index (BMI) and central nervous system (CNS) activity.Methods: An observational study was conducted in a series of HER2-negative ABC patients treated from January’14-December’17 outside a clinical trial. Objective Response Rate (ORR), Progression Free Survival (PFS), Overall Survival (OS), and association of clinical and pathological variables with outcome were evaluated. Results: Ninety-five women were treated with at least one cycle of eribulin. Median age was 57 (33-83), and 18% were obese. Median number of prior chemotherapies for ABC was 3 (2-5) and 76% of patients had visceral metastases, including 21% with CNS involvement. Most tumors were estrogen receptor-positive (79%). ORR and stable disease (SD) at 6 months were 26.2% and 37.5%, respectively. Remarkably, relevant CNS efficacy was observed with eribulin: 20% of patients obtained partial response and 25% SD. Treatment was generally well tolerated and manageable, with 29% grade 3 and 10.9% grade 4 toxicities. Median PFS and OS were 4.1 months (CI95% 3.2-4.9) and 11.1 months (CI95% 9.5-14.7), respectively. Triple-negative disease, >2organs involved and being younger than 70 years old were independent prognosis factors for worse OS in multivariate analysis. Most patients (75%) progressed in pre-existing metastases sites.Conclusions: In everyday clinical practice, eribulin’s efficacy seems similar to pivotal trials. CNS-efficacy was observed. TNBC, >2 organs involved and being younger than 70 years old were independent prognosis factors for worse OS. Remarkably, less incidence of grade 4-toxicity compared to previous studies was found.
Implementation of a National Electronic Health Information System in Gabon: A Survey of Healthcare Providers’ Perceptions.
Authors: Bagayoko C.O. , Tcheente Jack , Traoré Diakaridia , Moukoumbi Gaetan , Ondzigue Raymond , Koumamba Aimé Patrice , Ondjani Myriam Corille , Ndjeli Olive Lea , Gagnon Marie-Pierre
Published: September 2019

Background: Health information systems bring several benefits for the health system, healthcare providers, and the population. As a set of tools for the collection, storage, management and transmission of health data, their role to support the modernization of health systems, improve access to quality health care and reduce costs in developing countries is unquestionable. However, HIS implementation in low-income countries face several challenges. In Gabon, a unique initiative called eGabon has been launched in order to modernize the country’s infrastructures, notably through the deployment of a unique national electronic HIS that will connect health care institutions and providers at all levels in the whole country. Objective: This study aims to identify the factors influencing the optimal use of the national electronic HIS by healthcare providers in Gabon. Methods: We used an adaptation of the Information System Success Model ( and developed a questionnaire that was distributed to 2600 healthcare providers across the country). Reliability and validity of the instrument were tested, and we performed a logistic regression to identify the factors influencing healthcare providers’ perceptions towards the national electronic HIS. Results: A total of 2327 questionnaires were received from healthcare providers, of which 1930 were usable in the analyses. The reliability and validity of the questionnaire were supported. The logistic regression identified five constructs that significantly influence perceived system impact: System quality, Information quality, Support quality, Actual use and Useful functions. The model explains 30% of the variance in providers’ perception that the HIS leads to positive impacts. Discussion: This study provides support to the use of an adapted ISSM in the context of HIS implementation in a low-income setting. The results show that health care providers’ perceptions regarding the positive impact of the HIS are influenced by their previous use of a HIS, the extent of their use, their perceptions of system quality, information quality and quality of the support provided to users. These results could inform the development of strategies to ensure adequate change management and user experience for the implementation of the national electronic HIS, and eventually in other low-resources settings.
Construction of a lncRNA-associated ceRNA network and identification of a potential six-lncRNA prognostic signature for esophageal cancer
Authors: Hu Jun , Wang Fang , Liu Logen , Wenfeng Ning
Published: September 2019

BACKGROUND: Mounting evidence has shown that long noncoding RNAs (lncRNAs) can function as competing endogenous RNAs (ceRNAs) which participate in the initiation and progression of cancers. In the ceRNA network, lncRNAs, microRNAs (miRNAs) and mRNAs, communicate with and co-regulate each other. Rarely there is a systematic lncRNA-mediated ceRNA network and potential specific ceRNA pairs or triples of esophageal cancer (EC). In this study, we investigate the lncRNA-mediated ceRNA network in EC and screen the potential prognostic lncRNA biomarkers. METHODS: We obtained mRNA, miRNA, and lncRNA expression data and relevant clinical features on patients with EC from The Cancer Genome Atlas (TCGA), and used the edgR package to identify differentially expressed mRNAs, lncRNAs and miRNAs between EC samples and normal samples. The EC ceRNA network was constructed based on miRNA target prediction through the databases of miRcode, miRDB, miRTarBase and TargetScan. And then Pearson’s correlation analysis was adopted to identify co-expression mRNA-lncRNA pairs. Finally, the robust likelihood-based survival analysis and Cox regression models were used to identify prognosis-related lncRNAs, which was evaluated by Kaplan-Meier and receiver operating characteristic (ROC) curve analysis. RESULTS: A total of 3,200 mRNAs, 131 miRNAs and 1,338 lncRNAs were identified as significantly differentially expressed in EC, of which, 30 mRNAs, 15 lncRNAs, and 8 miRNAs were incorporated in the ceRNA network. According to the ceRNA network node degrees, lncRNA MAGI2-AS3, hsa-mir-93 and TGFBR2 were the key genes. Also, the ceRNA network revealed some important ceRNA pairs and triples, such as SNX29P2-TGFBR2 and MAGI2-AS-hsa-mir-143-COL1A1. Finally, we developed a six-lncRNA signature (ZNF341-AS1, AC130324.2, AC027271.1, AL591212.1, AL732314.4 and LOC105372352), with improved diagnostic potential for EC with the area under the ROC curve of 0.93. CONCLUSIONS: our present work sheds new light on the tumorigenesis roles of lncRNA-mediated ceRNA network in EC and identifies a six‐lncRNA model that could be used as candidate prognostic signature.
hCAIX Expression and Cell Cycle in Cytokine Stimulated Human Colon Cancer Cells
Authors: Sagkan Rahsan Ilikci , Kockar Feray , Sengul Ali , Yilmaz Sukran , Musabak Ugur
Published: September 2019

Background: At the aim of this study, we investigated the effects of different kind of cytokines and the combinations of which on human carbonic anhydrase IX ( hCAIX) expression in HT-29 cell selected as colon carcinoma model for different doses and time of exposure to determine role of cytokines for treatment of colon carcinoma cells. Results: To sum up, h CA9 expression in the levels of gene and protein increased in HT-29 cells when stimulated with 1000 U/mL TGF-β for 24 h. The stimulation of HT-29 cells with IL1 α alone and IL1α- TGF-β combination has not revealed any effect on hCA9 expression in both levels of gene and protein in contrast to, 1000 U/mL IL1α-TNFα and especially TGFβ-TNFα have reducing effect on h CA9 expression level in HT-29 cells for time ranges of 24 h, 48 h, 72 h. In addition to this data, it was observed that HT-29 cells at the phase of G0G1 are arrested in cell cycle when stimulated with either of 1000 U/mL IL1α-TNFα and TGFβ-TNFα. Moreover, it was observed that h CA9 expression level in HT-29 cells decreases at the phases of synthesis (S) and G2M. Discussion: We concluded that combination of TNFα-TGFβ has created antagonistic effect on hCA9 expression in HT-29 colon cancer cell model. On the other hand, combination of TNFα-IL1α caused sinergistic effect on h CA9 expression level in this cell model. When these results are demonstrated decrease in the cytokine exposed hCA9 expression is a finding to develop a novel approach for anticancer therapy. Conclusion: Our finding related to the change in the expression of hCA9 following cytokine stimulate to colon cancer cell line gives an idea about the effect of cytokine stimulation on the expression of this gene which will be a hot spot research in colon carcinogenesis. Methods: HT-29 colon cells were chosen as a colorectal adenocarcinoma model. hCA9 gene expression in the level of mRNA was measured in cytokines stimulated HT-29 cells by Quantitative Real-time PCR. Meanwhile, hCAIX expression in the level of protein and cell cycle aassay were detected by flow cytometry.
Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16
Authors: Ma Jian , Zhang Han , Li Shuiqin , Zou Yaya , Li Ting , Liu Jiajun , Ding Puyang , Mu Yang , Tang Huaping , Deng Mei , Liu Yaxi , Jiang Qiantao , Chen Guoyue , Kang Houyang , Li Wei , Pu Zhien , Wei Yuming , Zheng Youliang , Lan Xiujin
Published: September 2019

Background Kernel length (KL), kernel width (KW) and thousand-kernel weight (TKW) are key agronomic traits in wheat breeding. Chuannong16 (‘CN16’) is a commercial cultivar with significantly longer kernels that the line ‘20828’. To identify and characterize potential alleles from CN16 controlling KL, the previously developed recombinant inbred line (RIL) population derived from the cross ‘20828’ × ‘CN16’ and the genetic map constructed by the Wheat55K SNP array and SSR markers were used to perform quantitative trait locus/loci (QTL) analyses for kernel traits.Results A total of 11 putative QTL associated with kernel traits were identified and they were located on chromosomes 1A (2 QTL), 2B (2 QTL), 2D (3 QTL), 3D, 4A, 6A, and 7A, respectively. Among them, three major QTL, QKL.sicau-2D , QKW.sicau-2D and QTKW.sicau-2D , controlling KL, KW and TKW, respectively, were detected in three different environments. Respectively, they explained 10.88-18.85%, 17.21-21.49% and 10.01-23.20% of the phenotypic variance. Further, they were genetically mapped in the same interval on chromosome 2DS. A previously developed kompetitive allele-specific PCR (KASP) marker KASP-AX-94721936 was integrated in the genetic map and QTL re-mapping finally located the three major QTL in a 1- cM region flanked by AX-111096297 and KASP-AX-94721936 . Another two co-located QTL intervals for KL and TKW were also identified. A few predicated genes involved in regulation of kernel growth and development were identified in the intervals of these identified QTL. Significant relationships between kernel traits and spikelet number per spike and anthesis date were detected and discussed.Conclusions Three major and stably expressed QTL associated with KL, KW, and TKW were identified. A KASP marker tightly linked to these three major QTL was integrated. These findings provide information for subsequent fine mapping and cloning the three co-localized major QTL for kernel traits.
Genome-wide analysis of gibberellin-dioxygenases genes in rice
Authors: Liu Wei , Huang Jishuai , Xu Yanghong , Zhang Qiannan , Hu Jun
Published: September 2019

Background Gibberellins (GAs), a pivotal plant hormone, play fundamental roles in plant development and growth. In rice, gibberellin-dioxygenases (GAoxes), OsGA20ox, OsGA3ox and OsGA2ox, are involved in the biosynthesis and deactivation of gibberellins. However, a comprehensive genome-wide analysis of gibberellin-dioxygenases genes is still uncovered.Results In this study, a total of 95 candidate OsGAox genes were found and 19 OsGAox genes were further analyzed. Results of phylogenetic tree showed that the OsGAox genes in Arabidopsis and rice were divided into four subgroups and shared some common features. Furthermore, analysis of gene structure and conserved motifs revealed that splicing phase and motifs were well conserved during the evolution of GAox genes in Arabidopsis and rice, and some specific motifs still need to be further studied. Exploration of expression profiles indicated that most of OsGAox genes exhibited tissue-specific expression patterns, implied their complicated functions. Moreover, the expression patterns of these genes under GA 3 and PAC treatment were investigated, and result showed that some genes, OsGA2ox3, OsGA2ox5, OsGA2ox7, OsGA2ox9, OsGA20ox2, and OsGA20ox4, may play a major role in GA homeostasis to cope with exogenous GA.Conclusions Our study provided a comprehensive analysis of the OsGAox gene family. Splicing characteristics and conserved motifs indicated the evolutionary conserved function in plants. Expression profiles indicated that each OsGAox gene has complicated and special functions. Although plenty of GAoxes were involved in the endogenous GA metabolism, only some of them acted in response to the exogenous GA treatment, which provided available information for researchers to manipulate the chemical GAs to improve the plant architecture and production.
Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı
Authors: KARAKAŞ Özgür
Published: September 2019

Tryptantrin and
indirubin are pharmacologically active compounds used in treatment of disseases
such as cancer and Alzheimer's. In this study, we investigated the influences
of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on
tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in
vitro. In all media treated with methyl jasmonate, tryptanthrin production in
leaves of plantlets showed an increase. The highest increase in tryptanthrin
production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM
MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control
(untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin
decreased about 2.56-fold in the leaves of plantlets treated with Put, when
compared to control. The highest indirubin production was obtained in the leaves
of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg
g-1) but treatments with Put didn’t show any positive affect on the indirubin
production. Analysis of tryptanthrin and indirubin were performed using high
performance liquid chromatography (HPLC).
Investigation of Physicochemical Properties of Some Monofloral Honeys in South Western Anatolia
Authors: KARATAS Sukru , AKTUMSEK Abdurrahman , DURU Mehmet Emin
Published: September 2019

In this study, fourty monofloral honeys belonging to four sources (Chaste, thyme, citrus and heather) were obtained from South West Anatolia (Mugla, Aydin, Denizli and Antalya provinces). Firstly, pollen species of honeys were identified and categorized according to pollen concentrations. Then, physicochemical analyses of honey samples were carried out in terms of moisture, pH, free acidity, conductivity, diastase, proline and sugar profile. Physicochemical results of moisture values 15.04-19.52 %, density values 1.32-1.43 (g/cm3), viscosity values 5.81-11.49 25 0C/Pa.s, ash content 1.32-1.43 %, pH values 3.74-4.78, free acidity values 8.96-33.92 meq/kg, conductivity values 0.15-1.41 mS/cm, diastase numbers 3.44-17.26 g/100g, proline contents 204.06-1588.93 mg/kg and sugar contents (glucose+fructose) 62.02-74.90 %. It was shown that the results obtained when compared to TFC (Turkish Food Codex) and CODEX Alimentarius Commission Honey Standards were in line with the standards.
Research on cold mechanical vibration of air-cooled disc brake
Authors: Zhang Sen , Zhang Jian
Published: September 2019

Cold mechanical vibration of air-cooled brake disc is mainly caused by the non-uniform geometry, including disk thickness variation (DTV) and side face run-out (SFR). A mathematical model consisting of the non-uniform geometry is constructed, which is imported into the dynamic brake model established by Lagrange equation as initial parameters. The braking moment fluctuation during braking process is obtained by MATLAB software. The experimental verification is realized based on Link3900 NVH test platform, where result shows that the calculated brake pressure is quite consistent with the test value. By single variable method, the key influence factor that affected braking moment fluctuation is analyzed, including DTV, SFR, friction coefficient, contact stiffness coefficient and damping coefficient. The conclusion shows that reducing these parameters within a certain range can significantly reduce the amplitude of cold vibration, except for the contact damping coefficient.
BOBİ FRS Karşılıklar Standardı ve Uygulaması
Authors: Yıldırım Gülçin
Published: September 2019

Büyük ve Orta Boy
İşletmeler İçin Finansal Raporlama Standardı (BOBİ FRS), 29 Temmuz 2017 tarihli
ve 30138 Sayılı Mükerrer Resmi Gazete’de yayımlanmıştır. Bu standart, bağımsız
denetime tâbi olup tam set Türkiye Muhasebe Standartlarını (TMS) uygulamayan
işletmelerin 1 Ocak 2018’den itibaren uygulayacakları finansal raporlama çerçevesi
olma özelliğini taşımaktadır. Kamu Gözetimi Kurumu (KGK), TMS uygulama
kapsamını genel olarak Kamu Yararını İlgilendiren Kuruluş (KAYİK)’lerle
sınırlandırmıştır. KAYİK’ler dışında kalan ve TMS uygulamayan işletmelerin
finansal tablolarında uygulayacakları raporlama çerçevesi daha önce Muhasebe
Sistemi Uygulama Genel Tebliği (MSUGT) ve ilave bir tebliğ ile KGK tarafından
belirlenmiştir. Ancak uygulama eksikliklerinin giderilmesi ve finansal
tabloların karşılaştırılabilir, gerçeğe ve ihtiyaca uygun sunumunun sağlanması
amacıyla BOBİ FRS hazırlanmıştır. Bu çalışmada, BOBİ FRS Bölüm 19’da yer alan
karşılıklara ilişkin ilkeler detaylı bir şekilde ele alınmıştır.
Estratégias discursivas da Mídia Ninja no caso do habeas corpus (não) concedido ao ex-presidente Lula
Authors: Ramalho Raul , Maia Kênia
Published: September 2019

Este artigo analisa a produção de sentido do coletivo Mídia Ninja, na sua página no Facebook, no que diz respeito ao habeas corpus concedido (e depois negado) ao ex-presidente do Brasil, Luiz Inácio Lula da Silva, no dia 8 de julho de 2018. O trabalho utiliza como metodologia a Análise de Discurso baseada no modelo tridimensional de Fairclough (2001). A pesquisa problematiza o caráter midiativista da Mídia Ninja, ao concluir que o grupo atuou a favor de libertação de Lula, a partir de uma narrativa centralizada e multimidiática (textos escritos, fotos, charges, vídeos e emissões ao vivo) aliada a uma estratégia discursiva contra-hegemônica, elegendo adversários (a exemplo do juiz Sérgio Moro) e heróis (como Lula e o então pré-candidato à presidência, Guilherme Boulos), e apontando a situação como parte de um golpe articulado pela mídia tradicional e por setores das classes política e judiciária.
Broadening the Understanding of Art Education
Authors: Lutfi Dina A.M.
Published: September 2019

This paper aims to explore the possibilities of approaching art education teaching methods with pragmatism. In the traditional sense, the role of the art educator has been to develop technical and visual skills of students. Today, the art educator is capable of developing teaching methods to enrich minds. Through thoughtful educational experiences, art educators may teach their students that works of art hold within them various social, cultural, personal meanings, and interpretations, which are an extension of the limitless possibilities explored by those who practice art making. Art educators must take into consideration students’ capacity in artistic learning. The understanding of art will differ as a result of students’ exposure to art, in addition to their understanding of everyday life. To arrive at new and renewed approaches, educators may pose questions, such as, how may art educators continue to teach traditional art, which is valuable when it comes to understanding the artistic styles that form diverse cultural and social fabrics, while also introducing contemporary art practices? In what ways may art educators engage with their students, share their experiences and knowledge, while simultaneously presenting them with new challenges?
Relationship Between Salinity and Crop Production in Different Hydrological Regimes Downstream of Muhuri Regulator
Authors: Chowdhury A.K.M.Alauddin , Rahman Md. Habibur , Alam Md. Rashidul , Sattar Md. Abdus , Quddus Md. Abdul
Published: September 2019

A large landmass was formed at the downstream of Feni river due to the construction of Muhuri regulator at a cross dam in Mirsaraiupazila of Chittagong district. But this land is more vulnerable to storm and tidal surges leading to saline water intrusion. The study site was selected from this area to investigate the relationship between salinity and crop production. Soil samples were collected from different hydrological regimes such as old protected area, new protected area, unprotected area and affected area from topsoil, subsoil and substratum in both wet and dry period. Crop yield of the sampling plots were recorded through farmer’s interview. It was found that, salinity of the study area was less than 4 dS/m both in aman and rabi season. Rice is a semi-tolerant crop (it can tolerate salinity up to 4 dS/m). So no significant relationship between soil salinity and rice yield was observed. Farmers of this area mainly cultivate khesari crop in rabi season. A significant positive correlation (correlation coefficient 0.97) was found between the soil salinity and khesari yield in unprotected area. Water salinity inside the cross dam was around 0.1 dS/m (rabi season), which is considered excellent for irrigation. But outside the cross dam it ranged in between 4.8-5.4 dS/m in aman season and 12.4-20.8 dS/m in rabi season, which was extremely toxic for irrigation for the cultivation boro rice. Although soil salinity permits to cultivate HYV rice in a man season but water logging is the main constraint. HYV rice in boro season can be cultivated in old and new protected area by utilizing Feni river water inside the Muhuri regulator and closure dam for irrigation purpose. But in unprotected area, it can not be cultivated due lack of suitable irrigation water and tidal flooding. In the study area, non-irrigated crop like khesari can be grown successfully just after aman season by utilizing residual soil moisture.
Characteristics of Wolverine (Gulo gulo) dens in the lowland boreal forest of north-central Alberta
Authors: Jokinen Michael E. , Webb Shevenell M. , Manzer Douglas L. , Anderson Robert B.
Published: September 2019

We investigated Wolverine (Gulo gulo) denning ecology in the boreal forest of northern Alberta. During winters 2015/2016 and 2016/2017, we used live traps to capture four female Wolverines and fitted them with global positioning system (GPS) collars programmed to take a location every two hours. We determined reproductive status at capture and GPS location data were used to identify den sites. One female denned in one of the two years, one female denned in two consecutive years, and two females did not den during the study. Seven of the eight Wolverine den sites were in mature or old Black Spruce (Picea mariana) stands, where dens consisted of a hollow, moss-covered mound originating from a partially uplifted root mass caused by a leaning or fallen tree. One den was located under decayed logging debris with an overstorey dominated by dense deciduous regeneration. Maximum snow depth recorded (December–March) at weather stations in the study area was 32–51 cm. Spring snow coverage was scarce in our study area (<1%) and always associated with ice cover on lakes and large ponds; mean distance from dens to nearest spring snow coverage was 15.19 km (SD = 2.73, n = 8). Female Wolverines appear to be using locally-available denning structures in the lowland boreal forest, despite a lack of deep snow, persistent spring snow cover, or large boulders documented in other studies.
Pengaruh Kompensasi, Motivasi dan Disiplin Kerja Terhadap Kinerja Karyawan pada PT Livia Mandiri Sejati Banyuwangi
Authors: Istifadah Anissaul , Santoso Budi
Published: September 2019

Penelitian ini bertujuan unuk menganalisis pengaruh kompensasi, motivasi dan disiplin kerja terhadap kinerja karyawan pada PT Livia Mandiri Sejati Banyuwangi. Data yang digunakan dalam penelitian ini berupa data primer yang diperoleh melalui penyebaran kuesioner kepada karyawan PT Livia Mandiri Sejati Banyuwangi. Adapun yang menjadi populasi dalam penelitian ini adalah karyawan sebanyak 98 orang di PT Livia Mandiri Sejati Banyuwangi. Penentuan sampel menggunakan studi populasi. analisis  data menggunakan analisis regresi linear berganda. Berdasarkan hasil pengujian hipotesis dapat dinyatakan bahwa kompensasi berpengaruh terhadap kinerja karyawan pada PT Livia Mandiri Sejati Banyuwangi atau H1 diterima. Motivasi berpengaruh terhadap kinerja karyawan pada PT Livia Mandiri Sejati Banyuwangi atau H2 diterima. Disiplin kerja berpengaruh terhadap kinerja karyawan pada PT Livia Mandiri Sejati Banyuwangi atau H3 diterima.
PROSEDUR PENCATATAN PENERIMAAN DAN PENGELUARAN KAS PADA LEMBAGA PERKREDITAN DESA (LPD) DESA ADAT SULAYAH KECAMATAN SERIRIT
Authors: Dewi Ketut Catur Eka Sari
Published: September 2019

Penelitian ini bertujuan untuk mengetahui, Prosedur Pencatatan Penerimaan dan Pengeluran Kas Pada Lembaga Perkreditan Desa (LPD) Desa Adat Sulanyah Kecamatan Seririt.Teknik pengumpulan data yang digunakan yakni, metode wawancara dan dokumentasi. Objek penelitian adalah prosedur pencatatan penerimaan dan pengeluaran kas, dan subjek penelitian adalah Lembaga Perkreditan Desa (LPD) Desa Adat Sulanyah Kecamatan Seririt. Data yang digunakan adalah data kualitatif. Hasil penelitian menunjukan bahwa Prosedur Pencatatan Penerimaan dan Pengeluaran Kas Pada Lembaga Perkreditan Desa (LPD) Desa Adat Sulanyah Kecamatan Seririt ada 6 prosedur diantaranya : (1) Pencatatan Penerimaan Kas Pada Tabungan, (2) Pencatatan Penerimaan Kas Pada Deposito, (3) Pencatatan Pengeluaran Kas Pada Tabungan, (4) Pencatatan Pengeluaran Kas Pada Pencairan Deposito, (5) Pencatatan Pengeluaran Kas Pada Pencairan Kredit, dan (6) Pencatatan Penerimaan dan Pengeluaran Kas Akhir. Prosedur Pencatatan Penerimaan dan Pengeluaran Kas Pada Lembaga Perkreditan Desa (LPD) Desa Adat Sulanyah Kecamatan Seririt sudah sesuai dan memadai, ini dapat dilihat dari proses pencatatan penerimaan dan pengeluaran kas yang ada pada Lembaga Perkreditan Desa (LPD) tersebut. Kata Kunci : Prosedur, Penerimaan Kas, Pengeluaran Kas, Lembaga Perkreditan Desa (LPD).
PROSEDUR DAN SISTEM PENGENDALIAN INTERN DALAM PEMBERIAN KREDIT PADA KOPERASI SIMPAN PINJAM CIPTA MULIA DESA BONDALEM
Authors: Sasmitha Uun Ratdyanti
Published: September 2019

Penelitian ini bertujuan untuk mengetahui, (1) Prosedur Pemberian Kredit pada Koperasi Cipta Mulia Desa Bondalem, (2) Penerapan Sistem Pengendalian Intern dalam Pemberian Kredit pada Koperasi Simpan Pinjam Cipta Mulia Desa Bondalem. Teknik pengumpulan data yang digunakan yakni, metode wawancara, metode dokumentasi dan metode observasi. Subjek penelitian adalah KSP Cipta Mulia Desa Bondalem dan objek penelitian adalah prosedur dan sistem pengendalian intern dalam pemberian kredit. Data yang digunakan adalah data kualitatif. Hasil penelitian menunjukan bahwa (1) Prosedur Pemberian Kredit  KSP Cipta Mulia Desa Bondalem sudah baik dapat dilihat dari  prosedur pemberian kredit, bagian yang terkait dalam pemberian kredit, dokumen yang digunakan dalam pemberian kredit, catatan akuntansi yang digunakan dalam pemberian kredit yang dapat di gambarkan bagan aliran prosedur pemberian kredit ( flowchat  ), (2) Sistem Pengendalian Intern yang diterapkan pada Koperasi Simpan Pinjam Cipta Mulia Desa Bondalem dalam Pemberian Kredit tersebut sudah berjalan dengan baik, dibuktikan dengan diterapkannya unsur-unsur Sistem Pengendalian Intern. Kata Kunci       : Prosedur, Sistem Pengendalian Intern, Kredit dan Koperasi
ANALISIS LAPORAN ARUS KAS PADA LPD DESA PAKRAMAN PENGLATAN PERIODE 2013-2014
Authors: Yudiarta Kadek
Published: September 2019

Dalam mengawasi arus kas dibutuhkan laporan arus sehingga dapat diketahui perkembangan kas yang ada didalam LPD tersebut terkait dengan penerimaan kas maupun pengeluaran kas serta penilaian terhadap kegiatan usaha (operasi, investasi, dan pendanaan) yang terkait dengan kas. Penelitian ini bertujuan untuk mengetahui (1) laporan arus kas pada LPD Desa Pakraman Penglatan periode 2013-2014, dan (2) analisis laporan arus kas pada LPD Desa Pakraman Penglatan periode 2013-2014. Objek dalam penelitian ini adalah laporan arus kas pada LPD Desa Pakraman Penglatan dan analisis laporan arus kas pada LPD Desa Pakraman Penglatan. Pengumpulan data menggunakan metode dokumentasi. Kemudian data yang telah dikumpulkan diolah dengan menggunakan analisis deskriptif kuantitatif. Kata-kata kunci : Analisis, Laporan Arus Kas dan LPD Desa Pakraman Penglatan
PROSEDUR PEMBUKAAN REKENING TABUNGAN DAN REALISASI PENERIMAAN TABUNGAN PADA LEMBAGA PERKREDITAN DESA (LPD) DESA PAKRAMAN BONDALEM TAHUN 2013-2014
Authors: Sudiadnyani Komang Putri
Published: September 2019

Penelitian ini bertujuan untuk mengetahui bagaimana prosedur pembukaan rekening tabungan dan realisasi penerimaan tabungan pada LPD Desa Pakraman Bondalem. Teknik pengumpulan data yang digunakan yaitu metode wawancara dan metode dokumentasi. Subjek penelitian adalah LPD Desa Pakraman Bondalem dan objek penelitian adalah prosedur pembukaan rekening tabungan dan realisasi penerimaan tabungan. Jenis data yang digunakan adalah data kuantitatif dan kualitatif. Selanjutnya data akan dianalisis dengan analisis deskriptif kualitatif dan kuantitatif. Hasil penelitian menunjukkan bahwa (1) prosedur pembukaan rekening tabungan pada LPD Desa Pakraman Bondalem sudah sesuai dengan standar operasional prosedur pada LPD yang telah ditetapkan dan (2) realisasi penerimaan tabungan pada tabungan SIMADE mengalami penurunan diakibatkan karena tingkat suku bunga yang terlalu besar diantara produk tabungan yang lainnya dan penarikannya dilakukan setahun sekali, sedangkan pada tabungan SUKARELA dan Simpanan Berjangka (DEPOSITO) selalu mengalami kenaikan disebabkan karena tingkat suku bunga pada kedua program tabungan tersebut masih relatif rendah dan penarikannya bisa dilakukan sewaktu-waktu. Kata Kunci : Prosedur Pembukaan Rekening Tabungan, Realisasi
PERLAKUAN AKUNTANSI TERHADAP PENERIMAAN DAN PENGELUARAN KAS PADA LPD (LEMBAGA PERKREDITAN DESA) PAKRAMAN DHARMAJATI TUKADMUNGGA
Authors: Putrayasa Putu Agus
Published: September 2019

Penelitian bertujuan untuk mengetahui perlakuan akuntansi terhadap penerimaan dan pengeluaran kas serta penyajian kas kedalam laporan keuangan pada LPD Desa Pakraman Dharmajati Tukadmungga  Pengumpulan data menggunakan metode observasi, wawancara, dan dokumentasi. Data yang digunakan adalah data kualitatif berupa penjelasan perlakuan akuntansi terhadap penerimaan dan pengeluaran kas, sumber penerimaan kas dan pengeluaran kas, struktur organisasi beserta tugas dan wewenang dari masing-masing bagian serta sejarah berdirinya perusahaan. Selanjutnya data dianalisis dengan analisis deskriptif komparatif. Hasil penelitian menunjukkan bahwa (1) perlakuan akuntansi terhadap  penerimaan dan pengeluaran kas yang diterapkan oleh LPD Desa Pakraman Dharmajati Tukadmungga sesuai dengan Standar Akuntansi Keuangan yang berlaku umum, kas diakui atas dasar biaya historis, dimana kas dicatat sebesar nominal transaksi yang terjadi. (2) Laporan keuangan yang dihasilkan oleh LPD Desa Pakraman Dharmajati Tukadmungga adalah laporan neraca dan laporan laba/rugi, laporan yang berhubungan dengan kas adalah laporan neraca. Kata Kunci :     Perlakuan Akuntansi, Penerimaan , pengeluaran kas
Kontribusi Implementasi Manajemen Berbasis Nilai-Nilai Kearifan Lokal Tri Hita Karana, Kepemimpinan Pelayan Kepala Sekolah, Kecerdasan Spiritual, dan Kepuasan Kerja Terhadap Komitmen Organisasional Guru di SMP Negeri di Kecamatan Sawan Kabupaten Buleleng Provinsi Bali
Authors: Angga Dewi Putu Yulia , Gede Agung Anak Agung , Rihendra Dantes Kadek
Published: September 2019

Penelitian ini bertujuan untuk mengetahui besaran kontribusi antara variabel manajemen sekolah berbasis nilai-nilai kearifan lokal Tri Hita Karana, kepemimpinan pelayanan kepala sekolah, kecerdasan spiritual, kepuasan kerja dengan variabel komitmen organisasional guru. Penelitian ini tergolong jenis ex-post facto dengan rancangan analisis jalur (path analisis). Populasi dalam penelitian ini sejumlah 163 orang. Sampel penelitian diambil secara acak yang berjumlah 132 orang. Pengumpulan data menggunakan kuesioner dari variabel manajemen sekolah berbasis nilai-nilai kearifan lokal tri hita karana, kepemimpinan pelayanan kepala sekolah, kecerdasan spiritual, kepuasan kerja dan komitmen organisasional guru. Hasil penelitian menunjukkan bahwa seluruh hipotesis signifikan dan memiliki kontribusi terhadap komitmen organisasional. Sesuai dengan hasil analisis menunjukkan bahwa manajemen sekolah berbasis nilai-nilai kearifan lokal Tri Hita Karana, kepemimpinan pelayanan kepala sekolah, kecerdasan spiritual, dan kepuasan kerja secara simultan (bersama-sama) memberi kontribusi yang signifikan terhadap komitmen organisasional di SMP Negeri Kecamatan Sawan sebesar 0,914 atau 91,4%. Berdasarkan hasil analisis model regresi ŷ = 75,915 + 0,741 X1 + 0,826 X2 + 0,716 X3 + 0,732 X4 dengan Freg = 224,104 (p<0,05).Kata kunci: Manajemen Sekolah Berbasis Nilai-nilai Kearifan Lokal Tri Hita Karana, Kepemimpinan Pelayanan Kepala Sekolah, Kecerdasan Spiritual, Kepuasan Kerja dan Komitmen Organisasional Guru.