bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive Value of Pre-Operative 2D and 3D Transthoracic Echocardiography in Patients Undergoing Mitral Valve Repair: Long Term Follow Up of Mitral Valve Regurgitation Recurrence and Heart Chamber Remodeling
Authors: Tamborini Gloria , Mantegazza Valentina , Penso Marco , Muratori Manuela , Fusini Laura , Ghulam Ali Sarah , Cefalù Claudia , Italiano Gianpiero , Volpato Valentina , Gripari Paola , Caiani Enrico G. , Zanobini Marco , Pepi Mauro
Published: October 2020

The “ideal” management of asymptomatic severe mitral regurgitation (MR) in valve prolapse (MVP) is still debated. The aims of this study were to identify pre-operatory parameters predictive of residual MR and of early and long-term favorable remodeling after MVP repair. We included 295 patients who underwent MV repair for MVP with pre-operatory two- and three-dimensional transthoracic echocardiography (2DTTE and 3DTTE) and 6-months (6M) and 3-years (3Y) follow-up 2DTTE. MVP was classified by 3DTTE as simple or complex and surgical procedures as simple or complex. Pre-operative echo parameters were compared to post-operative values at 6M and 3Y. Patients were divided into Group 1 (6M-MR < 2) and Group 2 (6M-MR ≥ 2), and predictors of MR ≥ 2 were investigated. MVP was simple in 178/295 pts, and 94% underwent simple procedures, while in only 42/117 (36%) of complex MVP a simple procedure was performed. A significant relation among prolapse anatomy, surgical procedures and residual MR was found. Post-operative MR ≥ 2 was present in 9.8%: complex MVP undergoing complex procedures had twice the percentage of MR ≥ 2 vs. simple MVP and simple procedures. MVP complexity resulted independent predictor of 6M-MR ≥ 2. Favorable cardiac remodeling, initially found in all cases, was maintained only in MR < 2 at 3Y. Pre-operative 3DTTE MVP morphology identifies pts undergoing simple or complex procedures predicting MR recurrence and favorable cardiac remodeling.
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus
Authors: Samzadeh Mojdeh , Papuć Ewa , Furtak-Niczyporuk Marzena , Rejdak Konrad
Published: October 2020

The effects of status epilepticus on the orexin/hypocretin system have yet to be investigated. The present study aimed to assay orexin-A/hypocretin-1 in the cerebrospinal fluid (CSF) of patients after generalized convulsive status epilepticus (GCSE). The study groups included 20 GCSE patients, 24 patients diagnosed with epilepsy but remaining in remission (ER), and 25 normal controls (CTR). Diagnostic lumbar puncture was performed in GCSE patients within 3–10 days of seizure cessation, as well as in the ER and to CTR subjects. Among all GCSE patients, the outcome was graded according to the modified Rankin Scale (mRS) at 1-month follow-up. Orexin-A levels were measured in unextracted CSF samples, using a commercial radioimmunoassay. There was a significant overall difference in median CSF orexin-A concentrations between GCSE, RE, and CTR patients (p < 0.001). The lowest concentrations were noted in the GCSE group compared to ER (p < 0.001) or CTR (p < 0.001). CSF orexin-A levels in GCSE patients inversely correlated with clinical outcome as assessed on the mRS at 1-month follow-up (r = −0.55; p = 0.1). In conclusion, CSF orexin-A levels may serve as a biomarker of increased turn-over of the peptide or post-SE neuronal damage, and implicates the orexin system in the pathogenesis of SE.
A Novel Nomenclature for Repeat Motifs in the Thymidylate Synthase Enhancer Region and Its Relevance for Pharmacogenetic Studies
Authors: Schaerer Dominic , Froehlich Tanja K. , Hamzic Seid , Offer Steven M. , Diasio Robert B. , Joerger Markus , Amstutz Ursula , Largiadèr Carlo R.
Published: October 2020

Inhibition of thymidylate synthase (TS) is the primary mode of action for 5-fluorouracil (5FU) chemotherapy. TS expression is modulated by a variable number of tandem repeats in the TS enhancer region (TSER) located upstream of the TS gene (TYMS). Variability in the TSER has been suggested to contribute to 5FU-induced adverse events. However, the precise genetic associations remain largely undefined due to high polymorphism and ambiguity in defining genotypes. To assess toxicity associations, we sequenced the TSER in 629 cancer patients treated with 5FU. Of the 13 alleles identified, few could be unambiguously named using current TSER-nomenclature. We devised a concise and unambiguous systematic naming approach for TSER-alleles that encompasses all known variants. After applying this comprehensive naming system to our data, we demonstrated that the number of upstream stimulatory factor (USF1-)binding sites in the TSER was significantly associated with gastrointestinal toxicity in 5FU treatment.
Non-Dimensional Groups, Film Thickness Equations and Correction Factors for Elastohydrodynamic Lubrication: A Review
Authors: Marian Max , Bartz Marcel , Wartzack Sandro , Rosenkranz Andreas
Published: October 2020

Apart from complex numerical models to predict the tribological behavior of elastohydrodynamically lubricated contacts, non-dimensional similarity groups and analytically solvable proximity equations can be used to estimate integral fluid film parameters. Based upon the pioneering work presented by Dowson and Higginson as well as Blok and Moes, these approaches have been continuously improved over the years by modifications or correction factors to capture different contact geometries (line-, point- or elliptical contacts) as well as to include fluid compression, thermal, non-Newtonian, starvation or roughness effects. Consequently, this review article aims at systematically reviewing these modifications/corrections and discussing their applicability as well as limitations before presenting some recommendations for future research activities.
6-Bromo-N-(3-(difluoromethyl)phenyl)quinolin-4-amine
Authors: Asquith Christopher , Tizzard Graham
Published: October 2020

A routine synthesis was performed to furnish the title compound which incorporates a versatile difluoromethyl group on the aniline substitution of a 4-anilinoquinoline kinase inhibitor motif. In addition, the small molecule crystal structure (of the HCl salt) was solved, which uncovered that the difluoromethyl group was disordered within the packing arrangement and also a 126.08(7)° out of plane character between the respective ring systems within the molecule. The compound was fully characterized with 1H/13C-NMR and high-resolution mass spectra (HRMS), with the procedures described.
Factors Affecting the Behavioral Intention to Adopt Mobile Payment: An Empirical Study in Taiwan
Authors: Lin Wan Rung , Lin Chun-Yueh , Ding Yu-Heng
Published: October 2020

The convenience feature of mobile payment has replaced interactions with physical money and reduced transaction time, which better meets the demand of modern people for convenience in life. As mobile payments play an important role in mobile business, understanding the factors attracting consumers to mobile payment will bring mobile businesses more opportunities for development, and further significantly improve the output value of mobile businesses. This study discusses how to further influence consumer behavioral intention in Taiwan, as based on the main theoretical framework of the Extend Unified Theory of Acceptance and Use of Technology (UTAUT2) and Diffusion of Innovation (DOI). In this study, data analysis is implemented by Partial Least Squares (PLS) for the purpose of verifying the research model and hypotheses. The research results show that social influence, facilitating conditions, hedonic motivation, compatibility, innovation, relative advantage, and observability have positive influence on consumer intention to use mobile payment.
2D Nanocomposite Membranes: Water Purification and Fouling Mitigation
Authors: Loske Lara , Nakagawa Keizo , Yoshioka Tomohisa , Matsuyama Hideto
Published: October 2020

In this study, the characteristics of different types of nanosheet membranes were reviewed in order to determine which possessed the optimum propensity for antifouling during water purification. Despite the tremendous amount of attention that nanosheets have received in recent years, their use to render membranes that are resistant to fouling has seldom been investigated. This work is the first to summarize the abilities of nanosheet membranes to alleviate the effect of organic and inorganic foulants during water treatment. In contrast to other publications, single nanosheets, or in combination with other nanomaterials, were considered to be nanostructures. Herein, a broad range of materials beyond graphene-based nanomaterials is discussed. The types of nanohybrid membranes considered in the present work include conventional mixed matrix membranes, stacked membranes, and thin-film nanocomposite membranes. These membranes combine the benefits of both inorganic and organic materials, and their respective drawbacks are addressed herein. The antifouling strategies of nanohybrid membranes were divided into passive and active categories. Nanosheets were employed in order to induce fouling resistance via increased hydrophilicity and photocatalysis. The antifouling properties that are displayed by two-dimensional (2D) nanocomposite membranes also are examined.
The Future Application of Organ-on-a-Chip Technologies as Proving Grounds for MicroBioRobots
Authors: Fuller Haley , Wei Ting-Yen , Behrens Michael , Ruder Warren
Published: October 2020

An evolving understanding of disease pathogenesis has compelled the development of new drug delivery approaches. Recently, bioinspired microrobots have gained traction as drug delivery systems. By leveraging the microscale phenomena found in physiological systems, these microrobots can be designed with greater maneuverability, which enables more precise, controlled drug release. Their function could be further improved by testing their efficacy in physiologically relevant model systems as part of their development. In parallel with the emergence of microscale robots, organ-on-a-chip technologies have become important in drug discovery and physiological modeling. These systems reproduce organ-level functions in microfluidic devices, and can also incorporate specific biological, chemical, and physical aspects of a disease. This review highlights recent developments in both microrobotics and organ-on-a-chip technologies and envisions their combined use for developing future drug delivery systems.
SHRIMP U–Pb Zircon Ages, Geochemistry and Sr–Nd–Hf Isotope Systematics of the Zalute Intrusive Suite in the Southern Great Xing’an Range, NE China: Petrogenesis and Geodynamical Implications
Authors: Liu Huanan , Yuan Feng , Zhao Shengjin , Fan Mingjing , Guo Xiangguo
Published: October 2020

An integrated zircon geochronological, elemental geochemical, and Sr–Nd–Hf isotopic investigation was carried out on a suite of dioritic–granitic rocks at Zalute in the southern Great Xing’an Range (SGXR), NE China, in order to probe the source and petrogenesis of these granitoid rocks and further constrain the geodynamical setting of early Early Cretaceous magmatism. The results of Sensitive High-Resolution Ion Micro Probe (SHRIMP) zircon U–Pb dating reveal that the Zalute dioritic–granitic rocks have a consistent crystallization age of ca. 137–136 Ma, consisting of quartz diorite (136 ± 1.4 Ma), monzogranite (136 ± 0.8 Ma), and granite porphyry (137 ± 1.3 Ma), which record an early Early Cretaceous magmatic intrusion. Geochemically, the quartz diorites, monzogranites, and granite porphyries are mostly high-K calc-alkaline and show features of typical I-type affinity. They possess uniform and depleted Sr–Nd–Hf isotopic compositions (e.g., initial 87Sr/86Sr ratios of 0.7035 to 0.7049, εNd(t) of −0.02 to +2.61, and εHf(t) of +6.8 to +9.6), reflecting a common source, whose parental magma is best explained as resulting from the partial melting of juvenile source rocks in the lower crust produced by underplating of mantle-derived mafic magma, with minor involvement of ancient crustal components. Evidence from their close spatio–temporal relationship, common source, and the compositional trend is consistent with a magmatic differentiation model of the intermediate-felsic intrusive suite, with continued fractional crystallization from quartz diorites, towards monzogranites, then to granite porphyries. Combined with previously published data in the SGXR, our new results indicate that the Zalute intermediate-felsic intrusive suite was formed during the post-collisional extension related to the closure of the Mongol–Okhotsk Ocean and subsequent slab break-off.
Are Multicentre Bond Indices and Related Quantities Reliable Predictors of Excited-State Aromaticity?
Authors: Ponec Robert , Cooper David L. , Karadakov Peter B.
Published: October 2020

Systematic scrutiny is carried out of the ability of multicentre bond indices and the NOEL-based similarity index dAB to serve as excited-state aromaticity criteria. These indices were calculated using state-optimized complete active-space self-consistent field wavefunctions for several low-lying singlet and triplet states of the paradigmatic molecules of benzene and square cyclobutadiene and the inorganic ring S2N2. The comparison of the excited-state indices with aromaticity trends for individual excited states suggested by the values of magnetic aromaticity criteria show that whereas the indices work well for aromaticity reversals between the ground singlet and first triplet electronic states, addressed by Baird’s rule, there are no straightforward parallels between the two sets of data for singlet excited states. The problems experienced while applying multicentre bond indices and dAB to singlet excited states are explained by the loss of the information inherently present in wavefunctions and/or pair densities when calculating the first-order density matrix.
Rapid, Precise and Affordable Estimation of Venlafaxine and Its Metabolites in Highly Polluted Effluent Waters: Proof-of-Concept for Methodology
Authors: Hussain Snawar , Ramnarayanan Chandramouli , Roopashree Teeka S. , Anwer Md. Khalid , Sreeharsha Nagaraja , Nair Anroop B.
Published: October 2020

Widespread presence of pharmaceuticals and their metabolites in the environment of industrialized countries is an emerging global concern. Potential contamination of the soil and water by such pharmacologically active substances poses serious ecotoxicological implications. Several studies assessing the long-term ecological risks of pharmaceutical contaminants mainly focus on the risk assessment of the parent drug, while the potential contributions of their metabolites is often neglected. Presence of selective serotonin and norepinephrine reuptake inhibitor venlafaxine, an antidepressant drug, and its metabolites is a matter of serious concern for aquatic systems, since they are difficult to remove by traditional wastewater treatment processes. The concentration of VEN present in water is reportedly one of the highest among pharmaceuticals; however, the long-term effects of its metabolites have not yet been systematically studied. Given the consideration to complex and time-consuming effluent treatment, and realizing the importance of levels of venlafaxine and its metabolites, a simple and accurate analytical method for quick determination is needed. We designed a selective colorimetric method by using oxidative coupling of drug molecules with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) reagent, to quantify the presence of venlafaxine and its metabolites in aquatic samples, with special emphasis on effluent. The method was validated for selectivity, specificity and robustness as per the ICH Q2 guidelines to assess its suitability in pharmaceutical samples, as well. Highly sensitive and green economical analytical method was successfully established for estimation of venlafaxine and its metabolites in aquatic samples. The method was quick, as it involved minimum processing steps. The method was accurate and linear in the range of 0.5 to 80 ppm and could successfully detect lowest concentration of 1.3 ppm, thus qualifying its applicability for the desired purpose to check the presence of trace levels of VEN or its metabolites in aquatic samples or in pharmaceutical formulations.
Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis
Authors: Chen Jiali , Tang Cailin , Zhou Yang , Zhang Rongfei , Ye Shaoxia , Zhao Zhimin , Lin Ligen , Yang Depo
Published: October 2020

Cinnamomum camphora (Linn.) Presl has been widely used in traditional Chinese medicine for a variety of purposes. Our previous study indicated the antibacterial mechanism of the essential oil (EO) from C. camphora leaves; however, its anti-inflammatory activity and the underlying mechanism have not been clearly demonstrated. Thus, the present study investigated its anti-inflammatory property. Our data revealed that EO significantly decreased the release of nitric oxide (NO) and the mRNA expression of inducible NO synthase (iNOS) in lipopolysaccharide (LPS)-induced BV2 microglial cells. EO also attenuated LPS-induced increase in the mRNA expression and secretion of inflammatory cytokines including interleukin-6 (IL-6), IL-18, IL-1β and tumor necrosis factor-α (TNF-α). Furthermore, the metabolic profiles of LPS-induced BV2 microglial cells treated with or without EO were explored. Thirty-nine metabolites were identified with significantly different contents, including 21 upregulated and 18 downregulated ones. Five pathways were enriched by shared differential metabolites. Compared with the control cells, the glucose level was decreased, while the lactate level was increased, in the culture supernatant from LPS-stimulated cells, which were reversed by EO treatment. Moreover, compared to the LPS-treated group, the activities of phosphofructokinase (PFK) and pyruvate kinase (PK) in EO group were decreased. In summary, the current study demonstrated that EO from C. camphora leaves acts as an anti-inflammatory agent, which might be mediated through attenuating the glycolysis capacity of microglial cells.
Target-Guided Isolation of O-tigloylcyclovirobuxeine-B from Buxus sempervirens L. by Centrifugal Partition Chromatography
Authors: Szabó Lara U. , Schmidt Thomas J.
Published: October 2020

The increasing drug resistance of malaria parasites challenges the treatment of this life-threatening disease. Consequently, the development of innovative and effective antimalarial drugs is inevitable. O-tigloylcyclovirobuxeine-B, a nor-cycloartane alkaloid from Buxussempervirens L., has shown promising and selective in vitro activity in previous studies against Plasmodiumfalciparum (Pf), causative agent of Malaria tropica. For further investigations, it is indispensable to develop an advanced and efficient isolation procedure of this valuable natural product. Accordingly, we used liquid–liquid chromatography including centrifugal partition chromatography (CPC) to obtain the pure alkaloid on a semi-preparative scale. Identification and characterization of the target compound was accomplished by UHPLC/+ESI-QqTOF-MS/MS, 1H NMR and 13C NMR. In conclusion, this work provides a new and efficient method to obtain O-tigloylcyclovirobuxeine-B, a valuable natural product, as a promising antiplasmodial lead structure for the development of innovative and safe medicinal agents.
Protein-Stabilized Palm-Oil-in-Water Emulsification Using Microchannel Array Devices under Controlled Temperature
Authors: Kuroiwa Takashi , Ito Miki , Okuyama Yaeko , Yamashita Kanna , Kanazawa Akihiko
Published: October 2020

Microchannel (MC) emulsification for the preparation of monodisperse oil-in-water (O/W) and water-in-oil-in-water (W/O/W) emulsions containing palm oil as the oil phase was investigated for application as basic material solid/semi-solid lipid microspheres for delivery carriers of nutrients and drugs. Emulsification was characterized by direct observation of droplet generation under various operation conditions, as such, the effects of type and concentration of emulsifiers, emulsification temperature, MC structure, and flow rate of to-be-dispersed phase on droplet generation via MC were investigated. Sodium caseinate (SC) was confirmed as the most suitable emulsifier among the examined emulsifiers, and monodisperse O/W and W/O/W emulsions stabilized by it were successfully obtained with 20 to 40 µm mean diameter (dm) using different types of MCs.
Breakfast Characteristics and Its Association with Daily Micronutrients Intake in Children and Adolescents–A Systematic Review and Meta-Analysis
Authors: Giménez-Legarre Natalia , Miguel-Berges María L. , Flores-Barrantes Paloma , Santaliestra-Pasías Alba M. , Moreno Luis A.
Published: October 2020

Breakfast is an important source of key nutrients in the diet. For this reason, the aim of this review was to investigate the associations between breakfast consumption and daily micronutrients intake in both children and adolescents (aged 2–18 years). A peer-reviewed systematic search was conducted in three datasets (PubMed, Scopus and Cochrane Library) in February 2020 in English and Spanish. Two independent reviewers evaluated 3188 studies considering the AXIS critical appraisal and PRISMA methodologies. Meta-analysis was carried out comparing results according to type of breakfast consumed (Ready to eat cereals (RTEC) breakfast or other types of breakfast) and breakfast skipping. Thirty-three articles were included in the systematic review (SR) and 7 in the meta-analysis. In the SR, we observed that those children and adolescents who usually consume RTEC at breakfast had a higher consumption of B-vitamins than those not consuming RTEC at breakfast. Breakfast consumers had a higher mineral intake (iron, calcium, magnesium, potassium, zinc, and iodine) than breakfast skippers. In the Meta-Analysis, RTEC consumers had significantly higher vitamin C intake than breakfast skippers (Standard Mean Difference (SMD), −4.12; 95% confidence intervals (CI): −5.09, −3.16). Furthermore, those children who usually consume breakfast had significantly higher daily intake of calcium than breakfast skippers (SMD, −7.03; 95%CI: −9.02, −5.04). Our review proposes that breakfast consumption seems to be associated with higher daily micronutrients intake than breakfast skippers.
Antimicrobial Activities of Starch-Based Biopolymers and Biocomposites Incorporated with Plant Essential Oils: A Review
Authors: Syafiq R. , Sapuan S. M. , Zuhri M. Y. M. , Ilyas R. A. , Nazrin A. , Sherwani S. F. K. , Khalina A.
Published: October 2020

Recently, many scientists and polymer engineers have been working on eco-friendly materials for starch-based food packaging purposes, which are based on biopolymers, due to the health and environmental issues caused by the non-biodegradable food packaging. However, to maintain food freshness and quality, it is necessary to choose the correct materials and packaging technologies. On the other hand, the starch-based film’s biggest flaws are high permeability to water vapor transfer and the ease of spoilage by bacteria and fungi. One of the several possibilities that are being extensively studied is the incorporation of essential oils (EOs) into the packaging material. The EOs used in food packaging films actively prevent inhibition of bacteria and fungi and have a positive effect on food storage. This work intended to present their mechanical and barrier properties, as well as the antimicrobial activity of anti-microbacterial agent reinforced starch composites for extending product shelf life. A better inhibition of zone of antimicrobial activity was observed with higher content of essential oil. Besides that, the mechanical properties of starch-based polymer was slightly decreased for tensile strength as the increasing of essential oil while elongation at break was increased. The increasing of essential oil would cause the reduction of the cohesion forces of polymer chain, creating heterogeneous matrix and subsequently lowering the tensile strength and increasing the elongation (E%) of the films. The present review demonstrated that the use of essential oil represents an interesting alternative for the production of active packaging and for the development of eco-friendly technologies.
Removal of Cd2+ from Water by Use of Super-Macroporous Cryogels and Comparison to Commercial Adsorbents
Authors: Baimenov Alzhan , Berillo Dmitriy , Azat Seitkhan , Nurgozhin Talgat , Inglezakis Vassilis
Published: October 2020

In this study amphoteric cryogels were synthesized by the use of free-radical co-polymerization of acrylate-based precursors (methacrylic acid and 2-acrylamido-2-methyl-1-propansulfonic acid) with allylamine at different ratios. The physico-chemical characteristics of the cryogels were examined using SEM/EDX, FT-IR, XPS and zeta potential measurements. The cryogels were tested toward Cd2+ removal from aqueous solutions at various pH and initial concentrations. Equilibrium studies revealed a maximum sorption capacity in the range of 132–249 mg/g. Leaching experiments indicated the stability of Cd2+ in the cryogel structure. Based on kinetics, equilibrium and characterization results, possible removal mechanisms are proposed, indicating a combination of ion exchange and complexation of Cd2+ with the cryogels’ surface functional groups. The cryogels were compared to commercially available adsorbents (zeolite Y and cation exchange resin) for the removal of Cd2+ from various water matrices (ultrapure water, tap water and river water) and the results showed that, under the experimental conditions used, the cryogels can be more effective adsorbents.
Effect of a Novel Flame Retardant on the Mechanical, Thermal and Combustion Properties of Poly(Lactic Acid)
Authors: Niu Mingjun , Zhang Zhongzhou , Wei Zizhen , Wang Wanjie
Published: October 2020

Poly(lactic) acid (PLA) is one of the most promising biobased materials, but its inherent flammability limits its applications. A novel flame retardant hexa-(DOPO-hydroxymethylphenoxy-dihydroxybiphenyl)-cyclotriphosphazene (HABP-DOPO) for PLA was prepared by bonding 9,10-dihydro-9-oxy-10-phosphaphenanthrene-10-oxide (DOPO) to cyclotriphosphazene. The morphologies, mechanical properties, thermal stability and burning behaviors of PLA/HABP-DOPO blends were investigated using a scanning electron microscope (SEM), a universal mechanical testing machine, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), limiting oxygen index (LOI), vertical burning (UL-94) and a cone calorimeter test (CCT). The LOI value reached 28.5% and UL-94 could pass V-0 for the PLA blend containing 25 wt% HABP-DOPO. A significant improvement in fire retardant performance was observed for PLA/HABP-DOPO blends. PLA/HABP-DOPO blends exhibited balanced mechanical properties. The flame retardant mechanism of PLA/HABP-DOPO blends was evaluated.
NMPC-Based Workflow for Simultaneous Process and Model Development Applied to a Fed-Batch Process for Recombinant C. glutamicum
Authors: Levermann Philipp , Freiberger Fabian , Katha Uma , Zaun Henning , Möller Johannes , Hass Volker C. , Schoop Karl Michael , Kuballa Jürgen , Pörtner Ralf
Published: October 2020

For the fast and improved development of bioprocesses, new strategies are required where both strain and process development are performed in parallel. Here, a workflow based on a Nonlinear Model Predictive Control (NMPC) algorithm is described for the model-assisted development of biotechnological processes. By using the NMPC algorithm, the process is designed with respect to a target function (product yield, biomass concentration) with a drastically decreased number of experiments. A workflow for the usage of the NMPC algorithm as a process development tool is outlined. The NMPC algorithm is capable of improving various process states, such as product yield and biomass concentration. It uses on-line and at-line data and controls and optimizes the process by model-based process extrapolation. In this study, the algorithm is applied to a Corynebacterium glutamicum process. In conclusion, the potency of the NMPC algorithm as a powerful tool for process development is demonstrated. In particular, the benefits of the system regarding the characterization and optimization of a fed-batch process are outlined. With the NMPC algorithm, process development can be run simultaneously to strain development, resulting in a shortened time to market for novel products.
Geometric Variation in the Surface Rupture of the 2018 Mw7.5 Palu Earthquake from Subpixel Optical Image Correlation
Authors: Li Chenglong , Zhang Guohong , Shan Xinjian , Zhao Dezheng , Song Xiaogang
Published: October 2020

We obtained high-resolution (10 m) horizontal displacement fields from pre- and post-seismic Sentinel-2 optical images of the 2018 Mw7.5 Palu earthquake using subpixel image correlation. From these, we calculated the curl, divergence, and shear strain fields from the north-south (NS) and east-west (EW) displacement fields. Our results show that the surface rupture produced by the event was distributed within the Sulawesi neck (0.0974–0.6632°S) and Palu basin (0.8835–1.4206°S), and had a variable strike of 313.0–355.2° and strike slip of 2.00–6.62 m. The NS and EW displacement fields within the Palu basin included fine-scale displacements in both the near- and far-fault, the deformation patterns included a small restraining bend (localized shortening), a distributed rupture zone, and a major releasing bend (net extension) from the curl, divergence, and shear strain. Surface rupture was dominated by left-lateral strike-slip from initiation to termination, with a localized normal slip component peaking at ~3.75 m. The characteristics and geometric variation of the ruptured fault controlled both the formation of these surface deformation patterns and sustained supershear rupture.
Radar Satellite Image Time Series Analysis for High-Resolution Mapping of Man-Made Forest Change in Chongming Eco-Island
Authors: Xu Zhihuo , Wang Yuexia
Published: October 2020

Forests are important oxygen sources and carbon sinks. They provide a series of ecosystem services that are crucial to eco-island sustainability, such as the protection of soils, conservation of biodiversity, and development of the eco-tourism, etc. Interestingly, Chongming eco-island is located in the borderlands between fresh- and saltwater environments, where the Yangtze River meets the East China Sea. Most forests in Chongming island are therefore man-made and very vulnerable to the ecological environment mixing of freshwater streams and rivers with salty ocean tides, and are affected by climate and human activity. Estimating and monitoring forest change within an estuary is required for the sustainable management of forest resources and forest-based eco-tourism. Most optical satellites are unsuitable for continuous forest mapping due to cloud cover and their relatively low spatial and temporal resolution. Here, using Sentinel-1 satellite carrying an imaging C-band synthetic aperture radar, radar vegetation index was derived to detect the forest dynamics on the island. Furthermore we quantified forest area change in three well known and the most strictly protected and representative areas, namely Dongping National Forest Park, Dongtan National Wetland Park, and Xisha National Wetland Park, in the Chongming eco-island over the past five years at 10-metre resolution. We recorded the early and mid summers when the forest canopies grew to the peak in the study areas. The planted forest in Dongping National Forest Park grew an area of 7.38 hectares from 2015 to 2019, and disappeared from an area of almost 2.59 hectares in 2018. The man-made forest of Xisha National Wetland Park increased at an area of almost 20.19 hectares over the past five years. The forest in Dongtan National Wetland Park increased to an area of almost 2.12 hectares in the period of 2015–2017 and 2018–2019. However, from 2017 to 2018, the area of planted forests in Dongtan National Wetland Park decreased by 1.35 hectares. This study shows man-made forest change can be measured and that evidence can be collected to show how the forest is altered by human activities, and informs forest management decision-making for Chongming eco-island.
Reconciling Flagging Strategies for Multi-Sensor Satellite Soil Moisture Climate Data Records
Authors: van der Vliet Mendy , van der Schalie Robin , Rodriguez-Fernandez Nemesio , Colliander Andreas , de Jeu Richard , Preimesberger Wolfgang , Scanlon Tracy , Dorigo Wouter
Published: October 2020

Reliable soil moisture retrievals from passive microwave satellite sensors are limited during certain conditions, e.g., snow coverage, radio-frequency interference, and dense vegetation. In these cases, the retrievals can be masked using flagging algorithms. Currently available single- and multi-sensor soil moisture products utilize different flagging approaches. However, a clear overview and comparison of these approaches and their impact on soil moisture data are still lacking. For long-term climate records such as the soil moisture products of the European Space Agency (ESA) Climate Change Initiative (CCI), the effect of any flagging inconsistency resulting from combining multiple sensor datasets is not yet understood. Therefore, the first objective of this study is to review the data flagging system that is used within multi-sensor ESA CCI soil moisture products as well as the flagging systems of two other soil moisture datasets from sensors that are also used for the ESA CCI soil moisture products: The level 3 Soil Moisture and Ocean Salinity (SMOS) and the Soil Moisture Active/Passive (SMAP). The SMOS and SMAP soil moisture flagging systems differ substantially in number and type of conditions considered, critical flags, and data source dependencies. The impact on the data availability of the different flagging systems were compared for the SMOS and SMAP soil moisture datasets. Major differences in data availability were observed globally, especially for northern high latitudes, mountainous regions, and equatorial latitudes (up to 37%, 33%, and 32% respectively) with large seasonal variability. These results highlight the importance of a consistent and well-performing approach that is applicable to all individual products used in long-term soil moisture data records. Consequently, the second objective of the present study is to design a consistent and model-independent flagging strategy to improve soil moisture climate records such as the ESA CCI products. As snow cover, ice, and frozen conditions were demonstrated to have the biggest impact on data availability, a uniform satellite driven flagging strategy was designed for these conditions and evaluated against two ground observation networks. The new flagging strategy demonstrated to be a robust flagging alternative when compared to the individual flagging strategies adopted by the SMOS and SMAP soil moisture datasets with a similar performance, but with the applicability to the entire ESA CCI time record without the use of modelled approximations.
Vacant Parking Slot Recognition Method for Practical Autonomous Valet Parking System Using around View Image
Authors: Kim Seunghyun , Kim Joongsik , Ra Moonsoo , Kim Whoi-Yul
Published: October 2020

The parking assist system (PAS) provides information of parking slots around the vehicle. As the demand for an autonomous system is increasing, intelligent PAS has been developed to park the vehicle without the driver’s intervention. To locate parking slots, most existing methods detect slot markings on the ground using an around-view monitoring (AVM) image. There are many types of parking slots of different shapes in the real world. Due to this fact, these methods either limit their target types or use predefined slot information of different types to cover the types. However, the approach using predefined slot information cannot handle more complex cases where the slot markings are connected to other line markings and the angle between slot marking is slightly different from the predefined settings. To overcome this problem, we propose a method to detect parking slots of various shapes without predefined type information. The proposed method is the first to introduce a free junction type feature to represent the structure of parking slot junction. Since the parking slot has a modular or repeated junction pattern at both sides, junction pair consisting of one parking slot can be detected using the free junction type feature. In this process, the geometrically symmetric characteristic of the junction pair is crucial to find each junction pair. The entrance of parking slot is reconstructed according to the structure of junction pair. Then, the vacancy of the parking slot is determined by a support vector machine. The Kalman tracker is applied for each detected parking slot to ensure stability of the detection in consecutive frames. We evaluate the performance of the proposed method by using manually collected datasets, captured in different parking environments. The experimental results show that the proposed method successfully detects various types of parking slots without predefined slot type information in different environments.
Deletion of CD2-Like (CD2v) and C-Type Lectin-Like (EP153R) Genes from African Swine Fever Virus Georgia-∆9GL Abrogates Its Effectiveness as an Experimental Vaccine
Authors: Gladue Douglas P. , O’Donnell Vivian , Ramirez-Medina Elizabeth , Rai Ayushi , Pruitt Sarah , Vuono Elizabeth A. , Silva Ediane , Velazquez-Salinas Lauro , Borca Manuel V.
Published: October 2020

African swine fever virus (ASFV) is currently the most dreaded infectious disease affecting the global swine production industry. There is no commercial vaccine available, making the culling of infected animals the current solution to control outbreaks. Effective experimental vaccines have been developed by deleting virus genes associated with virulence. Deletion of the ASFV 9GL gene (∆9GL) has resulted in the attenuation of different ASFV strains, although the degree of attenuation varies across isolates. Here, we investigated the possibility of the increased safety of the experimental vaccine strain ASFV-G-Δ9GL by deleting two additional virus genes involved in pathogenesis, CD2v, a CD2 like viral encoded gene from the EP402R open reading frame (ORF), and C-type lectin-like viral gene, encoded from the EP153R ORF. Two new recombinant viruses were developed, ASFV-G-Δ9GL/ΔCD2v and ASFV-G-Δ9GL/ΔCD2v/ΔEP153R, harboring two and three gene deletions, respectively. ASFV-G-Δ9GL/ΔCD2v/ΔEP153R, but not ASFV-G-Δ9GL/ΔCD2v, had a decreased ability to replicate in vitro in swine macrophage cultures when compared with parental ASFV-G-Δ9GL. Importantly, ASFV-G-Δ9GL/ΔCD2v and ASFV-G-Δ9GL/ΔCD2v/ΔEP153R induced almost undetectable viremia levels when inoculated into domestic pigs and failed to protect them against challenge with parental virulent ASFV-Georgia, while ASFV-G-Δ9GL offered robust protection during challenge. Therefore, the deletion of CD2-like and C-type lectin-like genes significantly decreased the protective potential of ASFV-G-Δ9GL as a vaccine candidate. This study constitutes an example of the unpredictability of genetic manipulation involving the simultaneous deletion of multiple genes from the ASFV genome.
An Overview and Evaluation of Highly Porous Adsorbent Materials for Polycyclic Aromatic Hydrocarbons and Phenols Removal from Wastewater
Authors: Zango Zakariyya Uba , Sambudi Nonni Soraya , Jumbri Khairulazhar , Ramli Anita , Abu Bakar Noor Hana Hanif , Saad Bahruddin , Rozaini Muhammad Nur’ Hafiz , Isiyaka Hamza Ahmad , Osman Abubaker Mohammed , Sulieman Abdelmoneim
Published: October 2020

Polycyclic aromatic hydrocarbons (PAHs) and phenolic compounds had been widely recognized as priority organic pollutants in wastewater with toxic effects on both plants and animals. Thus, the remediation of these pollutants has been an active area of research in the field of environmental science and engineering. This review highlighted the advantage of adsorption technology in the removal of PAHs and phenols in wastewater. The literature presented on the applications of various porous carbon materials such as biochar, activated carbon (AC), carbon nanotubes (CNTs), and graphene as potential adsorbents for these pollutants has been critically reviewed and analyzed. Under similar conditions, the use of porous polymers such as Chitosan and molecularly imprinted polymers (MIPs) have been well presented. The high adsorption capacities of advanced porous materials such as mesoporous silica and metal-organic frameworks have been considered and evaluated. The preference of these materials, higher adsorption efficiencies, mechanism of adsorptions, and possible challenges have been discussed. Recommendations have been proposed for commercialization, pilot, and industrial-scale applications of the studied adsorbents towards persistent organic pollutants (POPs) removal from wastewater.
Application of the Systems Approach to the Management of Complex Water Systems
Authors: Simonovic Slobodan P.
Published: October 2020

This paper provides an introduction to, and an overview of, the Special Issue on the application of systems approach to the management of complex water systems. The main motivation in proposing this Special Issue was that today, more than ever, we need a systems approach to assist in dealing with the difficulties introduced by the increase in the complexity of water resource problems, consideration of environmental impacts, and the introduction of the principles of sustainability. This issue offers an opportunity to review applications of the systems approach to water resource management and draw lessons from worldwide experience relevant to future water problems. The Special Issue includes 15 contributions that offer an interesting view into contemporary problems, approaches, and issues related to management of complex water resources systems. It will be presumptuous to say that these 15 contributions characterize the success or failure of the systems approach to support water resources decision-making. However, these contributions offer some interesting lessons from the current experience and trace possible future work directions.
Improving Regulation and the Role of Natural Risk Knowledge to Promote Sustainable Low Enthalpy Geothermal Energy Utilization
Authors: De Giorgio Giorgio , Chieco Michele , Limoni Pier Paolo , Zuffianò Livia Emanuela , Dragone Vittoria , Romanazzi Annarita , Pagliarulo Rossella , Musicco Giuseppe , Polemio Maurizio
Published: October 2020

The use of geothermal energy resources to support anthropogenic activities have a long-lasting tradition, renewed in recent decades with the increasing use of low enthalpy geothermal energy (LEG) with combined systems of heat pumps and geothermal exchange, exploiting the enormous thermal capacity and very low temperature variability of subsoil, including rocks and water. The further potential global increase of LEG use could be enormous, although LEG is already the main geothermal energy sources in Europe, contributing significantly to reach 2030 UN sustainable development goals (SDGs) on renewable energy resources, as a further leg to support all necessary efforts for these scopes. This research pursues LEG spreading improving knowledge on limitations of guidelines, technical regulations and/or laws, briefly rules, especially in terms of potential risks or limitations due to environmental constrains or natural phenomena. A global documentary research, including scientific articles, books, technical reports from qualified institutions, technical standards, guidelines, regulations, and laws, was realized with three different groups of keywords. A total of 161 documents were selected after some steps, including quality check. Identical English and Italian keyword sets were used to span from an international global scale to the complex local scale which characterizes the Italian experience. A complex sheet was filled in for each document, supporting data discussion, planned with a geographical criterion, from global to local. The system of rules resulted worldwide inhomogeneous and complex, with high differences from countries, nations or regions, also at local scale. The low quality or the absence of simple and careful “rules” emerged an important obstacle to LEG diffusion that can guarantee sustainability and the absence of natural risks. Main virtuous systems of rules were recognized as very useful to promote LEG spreading but these are still uncommon. The discussion of optimal experiences and the overview of potential natural risks due to LEG complete the paper.
Uncoded chronic kidney disease in primary care: a cross-sectional study of inequalities and cardiovascular disease risk management
Authors: Molokhia Mariam , Okoli Grace N , Redmond Patrick , Asgari Elham , Shaw Catriona , Schofield Peter , Ashworth Mark , Durbaba Stevo , Nitsch Dorothea
Published: October 2020

Background

Uncoded chronic kidney disease (CKD) is associated with poorer quality of care.

Aim

To ascertain the proportion and determinants of CKD, which have not been formally recorded (Read coded), and identify differences in management and quality-of-care measures for patients with coded and uncoded CKD.

Design and setting

Cross-sectional survey undertaken in an ethnically diverse adult population using primary care electronic health records (EHRs) from GP clinics in Lambeth, South London, UK.

Method

Multivariable logistic regression analysis examined the association of demographic factors, selected comorbidities, deprivation, and cardiovascular disease risk management in CKD, with coding status as outcome.

Results

In total, the survey involved 286 162 adults, of whom 9325 (3.3%) were identified with CKD stage 3–5 (assigned as CKD based on estimated glomerular filtration rate [eGFR] values). Of those identified with CKD, 4239 (45.5%) were Read coded, and 5086 (54.5%) were uncoded. Of those identified with CKD stage 3–5, individuals aged ≥50 years were more likely to be coded for CKD, compared with those aged <50 years. Lower levels of coding were independently associated with deprivation and black Caribbean, black African, South Asian, and non-stated ethnicities, compared with white ethnicity. Prescribed statin and angiotensin-converting enzyme inhibitor/angiotensin receptor blocker medications were associated with increased odds of coded CKD.

Conclusion

This study found that >50% of CKD was uncoded and, for those patients, quality of care was lower compared with those with coded CKD. Future research and practices should focus on areas of greater deprivation and targeted initiatives for those aged <50 years and of black African, black Caribbean, South Asian, or non-stated ethnic groups. Possible areas for improvement include diagnostic coding support, automated CKD recording, and clinical decision support (based on adjusted eGFR results) in the GP clinical records.