bluuebunny/crossref_metadata_2025 huggingface dataset:
Pollinator Diversity and Pollination Efficiency of Stingless Bee Tetragonula iridipennis Smith in Bitter Gourd
Authors: Balaji K. , Jayaraj J , Shanthi M , Vellaikumar S , Rajamanickam C , Chitra N , Suresh K
Published: November 2023

An experiment was conducted out to investigate the pollinator diversity and pollination efficiency of Tetragonula iridipennis Smith on bitter gourd (Momordica charantia L.). In this study, there were 16 floral visitor species were recorded. Among them, six species were the most frequent visitors. A highest mean of visits was recorded with Halictus sp. (7.80) during 1000-1200 hrs in relative abundance and the lowest was of Megachile sp. (2.90) at 1600-1800 hrs. The foraging rate of stingless bees was most active in morning 1000-1200 hrs. The maximum fruit set was observed in pollinator-imposed fields (80.23 %) compared to pollinator excluded fields (71.15 %). Prominent increase in fruit length and girth in the pollinator included plots were respectively observed to be 249.19 and 42.91 mm. Maximum number of fruit/ individual plant (24.10) was achieved with bee pollination as compared to control (21.20).
De opschorting van Dublinoverdrachtstermijnen tussen effectieve rechterlijke bescherming en procedurele autonomie in
Authors: Hillary L.
Published: November 2023

De opschorting van Dublinoverdrachtstermijnen tussen effectieve rechterlijke bescherming en procedurele autonomie in

Uit de jurisprudentie van het Hof van Justitie blijkt dat de Nederlandse toepassing van de Dublinverordening, die bepaalt welke lidstaat verantwoordelijk is voor een asielverzoek, niet altijd in overeenstemming is met het beginsel van effectieve rechterlijke bescherming. Deze bijdrage behandelt specifiek de arresten S.S., N.Z., S.S. en E.N., S.S., J.Y. over de opschorting van Dublinoverdrachtstermijnen.

HvJ 30 maart 2023, C-338/21, ECLI:EU:C:2023:269 (S.S., N.Z., S.S.) en HvJ 30 maart 2023, C-556/21, ECLI:EU:C:2023:272 (E.N., S.S., J.Y.)
Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures
Authors: J.Y.* Lee , J.S. Choi , J.Y. Choi , Y.J. Shin , H. Yun , J.H. Cha , M.H. Chun , M.Y. Lee
Published: November 2023

We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen–glucose deprivation (OGD). The sequential induction pattern response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.
Influence of pediatric vaccines on amygdala growth and opioid ligand binding in rhesus macaque infants: A pilot study
Authors: L.* Hewitson , B. Lopresti , C. Stott , N.S. Mason , J. Tomko
Published: November 2023

This longitudinal, case-control pilot study examined amygdala growth in rhesus macaque infants receiving the complete US childhood vaccine schedule (1994-1999). Longitudinal structural and functional neuroimaging was undertaken to examine central effects of the vaccine regimen on the developing brain. Vaccine-exposed and saline-injected control infants underwent MRI and PET imaging at approximately 4 and 6 months of age, representing two specific timeframes within the vaccination schedule. Volumetric analyses showed that exposed animals did not undergo the maturational changes over time in amygdala volume that was observed in unexposed animals. After controlling for left amygdala volume, the binding of the opioid antagonist [11C]diprenorphine (DPN) in exposed animals remained relatively constant over time, compared with unexposed animals, in which a significant decrease in [11C]DPN binding occurred. These results suggest that maturational changes in amygdala volume and the binding capacity of [11C]DPN in the amygdala was significantly altered in infant macaques receiving the vaccine schedule. The macaque infant is a relevant animal model in which to investigate specific environmental exposures and structural/functional neuroimaging during neurodevelopment.
Chronic intermittent hypoxia exposure induces memory impairment in growing rats
Authors: Cai Xiao-Hong , Zhou Yong-Hai , Zhang Cun-Xue , Hu Liang-Gang , Fan Xiao-Fang , Li Chang-Chong , Zheng Guo-Qing , Gong Yong-Sheng
Published: November 2023

The study was aimed to examine the effect of chronic intermittent hypoxia (CIH) on spatial memory of growing rats and to explore the possible underlying mechanisms. Sixty two rats were trained to perform the 8-arm radial maze task and were divided into four groups: 2-week-CIH (2IH), 2-week-control (2C), 4-week-CIH (4IH) and 4-week-control (4C). There were more reference memory errors, working memory errors and total memory errors in 2IH and 4IH groups compared to the controls. The levels of 8-iso-Prostaglandin F(2-alpha) [8-ISO-PGF(2-alpha)], an in vivo marker for oxidative stress, in serum, hippocampus and prefrontal cortex were higher in CIH groups than the control groups. There were significant correlations between the levels of 8-ISO-PGF(2-alpha) and numbers of memory errors. The ultrastructural changes were evident in the hippocampal and prefrontal cortical tissues from the CIH groups. These results indicate that CIH can induce oxidative stress in brain tissues involved in spatial memory function.
Neural substrate of the cognitive and emotional interference processing in healthy adolescents
Authors: Mincic Adina
Published: November 2023

Emotion regulation is essential for adaptive functioning and social integration. However, it is not clear to what extent the responsible brain mechanisms are similar to those invoked in cognitive control in a non-emotional context. The aim of this study was to compare the neural circuitry of cognitive and emotional interference resolution in healthy adolescents, employing variants of the counting Stroop task. Cognitive and emotional interference processing engaged predominantly brain regions belonging to the dorsal- and the ventral attentional systems, respectively, and commonly the inferior frontal gyrus, IFG (Broca’s area, left BA 45, but also right BA 45). These results suggest that BA 45 is a bridge of interaction between the dorsal- and the ventral attentional systems implicated in top-down orienting of attention and, respectively, in bottom-up processing of salient stimuli. Reaction time data showed that some participants tend to respond faster, while others respond slower to negative emotional compared to neutral trials. The emotional interference maps revealed that fast responders recruit the right temporo-parietal junction and to a larger extent the right BA 45 and the bilateral cuneus, suggesting that they engage more efficient cognitive control mechanisms to override the attentional bias. No anterior cingulate (ACC) activation was observed in either cognitive, or emotional interference; this supports the view that ACC is not involved specifically in mediating Stroop selection.
Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells being at different level of neural commitment
Authors: Gornicka-Pawlak Elżbieta , Janowski Miroslaw , Habich Aleksandra , Jablonska Anna , Drela Katarzyna , Kozlowska Hanna , Lukomska Barbara , Sypecka Joanna , Domanska-Janik Krystyna
Published: November 2023

The aim of the study was to evaluate therapeutic effectiveness of intra-arterial infusion of human umbilical cord blood (HUCB) derived cells at different stages of their neural conversion. Freshly isolated mononuclear cells (D-0), neurally directed progenitors (D-3) and neural-like stem cells derived from umbilical cord blood (NSC) were compared. Focal brain damage was induced in rats by stereotactic injection of ouabain into dorsolateral striatum Three days later 107 of different subsets of HUCB cells were infused into the right internal carotid artery. Following surgery rats were housed in enriched environment for 30 days. Behavioral assessment consisted of tests for sensorimotor deficits (walking beam, rotarod, vibrissae elicited forelimb placing, apomorphine induced rotations), cognitive impairments (habit learning and object recognition) and exploratory behavior (open field). Thirty days after surgery the lesion volume was measured and the presence of donor cells was detected in the brain at mRNA level. At the same time immunohistochemical analysis of brain tissue was performed to estimate the local tissue response of ouabain injured rats and its modulation after HUCB cells systemic treatment. Functional effects of different subsets of cord blood cells shared substantial diversity in various behavioral tests. An additional analysis showed that D-0 HUCB cells were the most effective in functional restoration and reduction of brain lesion volume. None of transplanted cord blood derived cell fractions were detected in rat’s brains at 30th day after treatment. This may suggest that the mechanism(s) underlying positive effects of HUCB derived cell may concern the other than direct neural cell supplementation. In addition increased immunoreactivity of markers indicating local cells proliferation and migration suggests stimulation of endogenous reparative processes by HUCB D-0 cell interarterial infusion.
Expression of purinergic P2X(7) receptor in rat brain during the symptomatic phase of experimental autoimmune encephalomyelitis and after recovery of neurological deficits
Authors: Grygorowicz Tomasz , Sulejczak Dorota , Struzynska Lidia
Published: November 2023

Purinergic ionotropic P2X7 receptor is widely distributed in brain. Strong evidence suggests that this receptor is related to inflammatory and neurodegenerative changes in many pathological states of central nervous system (CNS), including multiple sclerosis (MS). Experimental autoimmune encephalomyelitis (EAE) is the commonly used animal model of MS. In this study we investigate the expression of P2X(7)R protein in rat brain in the symptomatic phase of EAE (day 10 post immunization) and after reversion of neurological symptoms (day 20 p.i.). We found the increased level of P2X(7)R protein in brain homogenates of EAE rats in both examined time windows. Immunohistochemical study revealed enhanced receptor’s immunoreactivity. Immunoblots done with isolated cellular brain fractions indicated that the P2X7R overexpression is related to synaptosomal fraction in the symptomatic phase and to the glial (GPV) fraction in the recovery phase of EAE. Concomitantly, we noticed overexpression of astroglial marker GFAP in brain homogenates and astroglial fraction (GPV), so as its enhanced immunoreactivity in brain sections (10 days p.i.) which did not decline to control values in the recovery phase, similarly to P2X(7)R expression. Results suggest the involvement of P2X(7)R-mediated signaling in the pathomechanisms of EAE with the possible relevance of astrocytic pool of cells.
Stroke induced brain changes: Implications for stem cell transplantation
Authors: Jablonska Anna , Lukomska Barbara
Published: November 2023

Ischemic stroke remains a major health problem associated with high mortality and severe morbidity. In spite of the extensive research in the field of stroke biology, there is little effective treatment for a completed disease onset. Numerous neuroprotective strategies have failed in clinical trials because of lack of efficacy or unacceptable side-effects. The challenge of clinical trial design is now to understand the process leading to ischemic brain injury and thus identify the targets for intervention in stroke. Therefore, uncovering cellular and molecular processes involved in ischemic brain injury is of critical importance. The review discusses the current understanding of these processes engaged in pathogenesis of stroke including excitotoxicity and inflammation. In addition recruitment of endogenous progenitors engaged in neurogenesis and vascular regeneration has been implicated. All of the aforementioned changes opted for therapeutic intervention to protect neurons in the region adjacent to the ischemic cerebral tissue and enhance cell recovery. Stem cell transplantation seems to offer a major promise of this therapy in stroke disorders.
Regulation of neurogenesis by extracellular matrix and integrins
Authors: Wojcik-Stanaszek Luiza , Gregor Anna , Zalewska Teresa
Published: November 2023

Deciphering the factors that regulate human neural stem cells will greatly aid in their use as models of development and as therapeutic agents. The complex interactions of cells with extracellular matrix (ECM) proteins probably contribute to proper central nervous system development mediating processes which regulate proliferation and differentiation of neural stem/rogenitor cells. Many of these interactions involve transmembrane integrin receptors. Integrins cluster in specific cell-matrix adhesions to provide dynamic links between extracellular and intracellular environments by activation of numerous signal transduction pathways which may influence cell behaviour profoundly by influence on both gene expression and post-transcriptional signalling cascade. In this review we introduced and discussed a number of extracellular and intracellular factors engaged in the transduction of signals induced by cell adhesion to its environment, including matrix components, extracellular proteolytic enzymes, integrins and non-receptor tyrosine kinases.
Relief of carotid stenosis improves impaired cognition in a rat model of chronic cerebral hypoperfusion
Authors: Duan Wei , Zhang Chun-Qing , Zheng Jian , Gui Li , Huang He-Qing , Chen Kang-Ning
Published: November 2023

To investigate how cognitive impairment is affected by the relief of bilateral carotid stenosis, chronic cerebral hypoperfusion was established through stenosis of the bilateral carotid common artery in adult Sprague-Dawley rats. Subsequently, the model rats received the intragastric placebo, donepezil (5 mg per kg), or surgery to relieve carotid stenosis after bilateral carotid common artery stenosis. After carotid stenosis was relieved, the cerebral blood flow values significantly increased, and P300 latency and escape latency in the Morris water-maze were significantly shortened. The concentrations of acetylcholine and norepinephrine in the dorsal hippocampus increased after carotid stenosis was relieved. Furthermore, P300 latency and escape latency were shortened in the relief-treated group compared to the drug-treated group, and acetylcholine levels in the relief-treated group were higher than the drug-treated group. No significant difference was found for the norepinephrine levels in the dorsal hippocampus between the relief-treated and drug-treated groups. Cognitive impairment can be significantly reduced by bilateral carotid stenosis relief, and the effect of relieving stenosis on cognitive dysfunction is superior to the effect of administering an acetylcholinesterase inhibitor.
Assessing changes in pial artery resistance and subarachnoid space width using a non-invasive method in healthy humans during the handgrip test
Authors: Wszedybyl-Winklewska Magdalena , Frydrychowski Andrzej , Winklewski Pawel
Published: November 2023

The aim of this study was to assess the influence of the handgrip test (HGT) on: (1) pial artery pulsation (cc-TQ), (2) subarachnoid space (SAS) width (sas-TQ) and (3) the relationship between peripheral blood pressure (BP), heart rate (HR), cerebral blood flow velocity (CBFV), resistive index (RI), cc-TQ and sas-TQ. The study was performed on 29 healthy volunteers (11 men and 18 women) with a mean age of 29.3 +/- SE 4.0. HGT was performed in the sitting position at 30% of maximal voluntary contraction. cc-TQ and sas-TQ were registered using near-infrared transillumination/backscattering sounding (NIR-T/BSS); BP and heart rate (HR) were measured using a Finapres monitor. CBFV and RI were recorded using a transcranial Doppler. A significant reduction in cc-TQ (-34.3 percent, P
Methylprednisolone improves the survival of new neurons following transient cerebral ischemia in rats
Authors: Jing Yu , Hou Yi , Song Yan , Yin Jie
Published: November 2023

Cerebral ischemia induced the proliferation and differentiation of neural stem cells in discrete regions of brain. However, only a small fraction of the neural stem/progenitor cells survives. In this report, the effects of methylprednisolone (MP) on proliferation, differentiation and survival of neural progenitor cells were explored through early MP administration after occlusion of the middle cerebral artery (MCAo). Transient cerebral ischemia was induced by middle cerebral artery occlusion in Sprague Dawley male rats. One hour, 1 day, 3 days, 14 days, and 28 days after MCAo, neurological examination was performed to evaluate the neurological deficit. MCAo rats were randomly divided into two groups, MP-group was injected MP (30 mg/kg, i.p.) at 3 h, 12 h, and 24 h after MCAo, and vehicle group was injected equal saline (i.p.). Animals were sacrificed at 3 days, 14 days, and 28 days after MCAo. MP was found to decrease apoptosis and TNF-alpha and IL-6 expression at 3 days after MCAo in the ipsilateral striatum. Moreover, MP significantly increased the migrated new neurons (BrdU+/ DCX+) and immature neurons (BrdU+/Tuj1+) at day 14 after MCAo in the ipsilateral striatum. Likewise, MP increased the number of mature neurons (BrdU+/MAP2+) at 28 days after MCAo. However, MP did not affect the progenitor cell proliferation (BrdU+ and Nestin+) at day 3 after MCAo in the subventricular zone (SVZ), but improved the neurological deficit at day 1 and day 3 after MCAo. These results indicate that early MP administration can improve the neurological deficit and enhance the survival of new neurons in the ipsilateral striatum by inhibiting apoptosis and downregulation of inflammatory response after transient cerebral ischemia in rats.
The catechol-O-methyltransferase and monoamine oxidase B polymorphisms and levodopa therapy in the Iranian patients with sporadic Parkinson’s disease
Authors: Torkaman-Boutorabi Anahita , Shahidi Gholam , Choopani Samira , Rezvani Mohammad , Pourkosary Kosar , Golkar Majid , Zarrindast Mohammad-Reza
Published: November 2023

Parkinson’s disease (PD) patients vary widely in their response to levodopa treatment, and this may be partially genetic in origin. Recent studies suggest that catechol-O-methyltransferase (COMT), G1947A and monoamine oxidase B (MAOB), A644G polymorphisms might influence the risk and treatment of PD. Herein, we aimed to test the possible influence of MAOB and COMT genetic polymorphisms on the effective daily dose of levodopa administered in the fifth year of treatment. We also examined the effect of COMT and MAOB haplotypes on levodopa therapy outcome. There were 31 females and 72 males of Iranian origin diagnosed with sporadic PD included into the study. The patients were divided into two groups. Group 1: patients received daily doses of levodopa below 500 mg in the fifth year of treatment. Group 2: those patients receiving daily doses exceeding 500 mg in the fifth year of treatment. MAOB and COMT polymorphism genotyping was performed by using PCR-based restriction fragment length polymorphism (RFLP) analyses. Our data show that the first group suffered less frequently from dyskinesia than patients from the second group. No statistically significant differences were found in allele frequencies and genotype distributions of the studied genes between two groups. In addition, the incidence of the specific haplotypes between the two groups did not show any difference. The present data suggest that pharmacokinetic or pharmacodynamic factors other than the investigated genetic variants of the MAOB and COMT enzymes seem to determine the response to levodopa in the Iranian PD patients.
Electrophysiological differences between high and low frequency rTMS protocols in depression treatment
Authors: Valiulis Vladas , Gerulskis Giedrius , Dapsys Kastytis , Vistartaite Giedre , Siurkute Aldona , Maciulis Valentinas
Published: November 2023

Repetitive transcranial magnetic stimulation (rTMS) is a rapidly expanding mean in drug resistant depression treatment. Yet, despite vast research in this field, exact neurophysiological mechanism of rTMS therapy still remains unclear. This results in difficulties choosing suitable rTMS parameters in advance and compromises thorough evaluation of efficacy after the treatment. In order to obtain more explicit assessment of rTMS therapy in the psychiatric field, we evaluated and compared the influence of two most widely used antidepressive rTMS protocols on EEG band power spectrum and relation to clinical test scores (MADRS, BDI, HAM-D17). Forty-five patients (12 male, 33 female, mean age 52.16 years) participated in the study. Twenty-three patients received high frequency (10 Hz) stimulation, the rest 22 were stimulated using low frequency (1 Hz) protocol. Both groups received 10 to 15 daily rTMS sessions. EEG recordings and clinical tests were obtained the day before rTMS course and same day after the last session. Majority (57.78%) of patients showed considerable improvement after the treatment. There were no notable differences in clinical test score drop between the two rTMS protocols. However, we found that different protocols resulted in significantly different electrophysiological changes. High frequency (10 Hz) rTMS resulted in widespread changes off EEG band power, including delta power increase on the left hemisphere and alpha power growth on the right. Theta power increase was also obtained in parietal-occipital areas. Low frequency (1 Hz) rTMS showed to have no major effect on basic EEG band power, however, we found a notable shift of frontal alpha power asymmetry towards the right hemisphere, which correlated with the clinical outcome. Our study results suggest that two widely used rTMS protocols strongly differ in their electrophysiological mechanisms. Low frequency stimulation finesse on frontal alpha power asymmetry shift, whereas high frequency protocol acts on wider electrophysiological changes in the brain.
Epigenetic mechanisms of gene expression regulation in neurological diseases
Authors: Gos Monika
Published: November 2023

Neurological diseases are a heterogenous group of disorders that are related to alterations in nervous system function. The genetic background of neurological diseases is heterogenous and may include chromosomal aberrations, specific gene mutations and epigenetic defects. This review is aimed at presenting of selected diseases that are associated with different epigenetic alterations. The imprinting defects on chromosome 15 are the cause of Prader-Willi and Angelman syndromes that both are characterized by intellectual disability, developmental delay and specific behavioral phenotype. Besides the imprinting defect, these diseases can also be caused by deletion of chromosome 15 or uniparental disomy. Aberrant epigenetic regulation is also specific for Fragile X syndrome that is caused by expansion of CGG repeats in the FMR1 gene that leads to global methylation of the promoter region and repression of FMR1 transcription. A number of neurological diseases, mainly associated with intellectual impairment, may be caused by mutations in genes encoding proteins involved in epigenetic regulation. The number of such diseases is rapidly growing thanks to the implementation of genomic sequencing for the identification of their molecular causes. One of the best known diseases linked to defects in epigenetic modifiers is Rett syndrome caused by a mutation in the MECP2 gene or its variant – Rett-like syndrome caused by a mutation in CDKL5 or FOXG1 genes. As the epigenetic signature is potentially reversible, much attention is focused on possible therapies with drugs that influence DNA or histone modifications. This is especially important in the case of neurological disorders in which epigenetic changes are observed as the effect of the disease.
Hippocampal interleukin-1beta in the juvenile and middle-aged rat: Response to chronic forced swim or high-light open-field stress stimulation
Authors: Badowska-Szalewska Ewa , Ludkiewicz Beata , Sidor-Kaczmarek Justyna , Lietzau Grazyna , Spodnik Jan , Swietlik Dariusz , Domaradzka-Pytel Beata , Morys Janusz
Published: November 2023

It is postulated that stress differentially affects interleukin-1beta (IL-1beta) during ontogenetic life. This study examined the influence of chronic exposure to forced swim (FS) stress or high-light open-field (HL-OF) stress on interleukin-1beta (IL-1beta). The total level of IL-1beta protein was assessed by Western blot analysis of hippocampal extracts. Double immunofluorescence staining was used to reveal the percentage of IL-1beta/NeuN (NeuN – neuronal marker) cells in the CA1, CA3 and dentate gyrus (DG) hippocampal subfields. Juvenile (P28; P – postnatal day) and middle-aged (P360) rats were used in the experiment. The research showed no significant differences in IL-1beta protein levels between P28 and P360 non-stress rats. However, a substantial increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG in P360 rats was observed. Chronic FS had no significant influence on IL-1beta expression in the hippocampus or on the percentage of IL-1beta-ir neurons in CA1, CA3 and DG hippocampal subfields in either age group. During HL-OF, the IL-1beta level was significantly increased in the hippocampus of P28 and P360 rats, whereas a marked increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG hippocampal areas occurred only in P360 animals. These results indicate that chronic HL-OF stimulation was the factor inducing changes in the IL-1beta protein levels in P28 and P360 rats and in the percentage of IL-1beta/NeuN-ir cells in the hippocampus of P360 animals.
Expression pattern of transcription factor SOX2 in reprogrammed oligodendrocyte precursor cells and microglias: Implications for glial neurogenesis
Authors: Zhang Ye , Yao1 Xiu-ming , Liu Zhao-hui , Xia Chun-lin
Published: November 2023

Oligodendrocyte Precursor Cells (OPCs) can revert to multipotential Neural Stem-Like Cells (NSLCs) which can self-renew and give rise to neurons, astrocytes and oligodendrocytes when exposed to certain extracellular signals. This is a significant progress to understand developmental neurobiology, in particularly the possibility of converting glia to stem cells for the treatment of neurological disorders. Similarly, recent findings revealed that brain-resident microglias (MGs) can be converted to multipotential state through de-differentiation. In this study, we investigated the role of SRY (sex-determining region)-box 2 (SOX2), a high-mobility group DNA binding domain transcription factor, in the reprogramming of OPCs and MGs and molecular pathways involved in these process. Immunocytochemical analyses demonstrated that expression of SOX2 was upregulated in the reprogrammed MGs and OPCs as well as other neural stem cell markers such as CD15 and nestin. Western blot and double immunostaining analyses further confirmed that activation of bone morphogenetic proteins (BMPs) signaling partnering with SOX2 might be one of the molecular pathways involved in lineage reprogramming of OPCs which is also true in the reversion of MGs. Taken together, these results indicated that lineage reprogramming of OPCs and MGs are both controlled by the same signaling pathway and glia can be reprogrammed in culture by inducing expression of neurogenic transcription factors to transgress their lineage restriction and can stably acquire a neuronal identity. Our results suggested innovative perspectives for cell therapy with glia cells.
40 Hz auditory steady-state response in females: When is it better to entrain?
Authors: Griskova-Bulanova Inga , Griksiene Ramune , Korostenskaja Milena , Ruksenas Osvaldas
Published: November 2023

Auditory steady-state responses (ASSRs) are widely applied to test brain’s ability to follow external stimulation in neuropsychiatric disorders. It is known that ASSRs are related to GABAergic transmission. Female sex steroid homones – both estrogens and progesterone – affect functioning of GABAergic system. However, it is not known how these hormones affect brain’s ability to entrain. This study was designed to test the ability to synchronize to 40 Hz stimulation during different phases of the menstrual cycle. Twenty-eight healthy females participated in the research during one of the menstrual cycle phases: (1) early follicular; (2) late follicular; (3) and mid-luteal. Auditory 40 Hz trains of 500 ms were delivered binaurally and EEG was recorded. Time-frequency analysis of the data was performed and phase-locking index, evoked amplitude and total intensity measures were extracted and decomposed by non-negative multi-way factorization. Additionally, alpha power of the baseline period was calculated. Parameters of ASSR were increasing in a linear manner with increasing levels of 17Beta-estradiol and largest estimates of ASSR parameters were obtained in the late follicular phase, smallest – in the mid-luteal phase. Alpha power values were highest in the late follicular phase and lowest in the mid-luteal phase, pointing to lower arousal level in the late follicular phase. We speculate that increased 40 Hz ASSRs during mid-cycle might be related to the level of general arousal and specific GABA-mediated changes during the menstrual cycle. The results suggest that the ability to entrain to 40 Hz stimulation depends on the phase of menstrual cycle. This should be taken into account, particularly when ASSRs are used in clinical practice, comparing patients and healthy subjects.
Gender differences in the neurochemical response of trigeminal ganglion neurons to peripheral inflammation in mice
Authors: Kuzawińska Olga , Lis Krzysztof , Cudna Agnieszka , Bałkowiec-Iskra Ewa
Published: November 2023

It is well established that the majority of headache and other trigeminal nerve-associated disorders have higher prevalence in females than in males. However, the pathogenesis of many chronic trigeminal pain conditions, such as trigeminal neuralgia, migraine and temporo-mandibular disorders, is still not known. One of the proposed mechanisms involve calcitonin gene-related peptide (CGRP), which is considered the most important neuropeptide in the trigeminal system. In various animal models of trigeminal nerve-associated disorders concentration of CGRP has been shown to be increased in trigeminal ganglia (TG). Moreover, intraganglionic release of CGRP has been shown to modulate neuronal transmission of pain signals. In most of these models, pathological changes in the trigeminal system are accompanied by inflammation within peripheral endings of TG neurons. The aim of the present study was to investigate the relation between gender and neurochemical changes in trigeminal ganglia evoked by peripheral inflammation, induced by Complete Freund Adjuvant (CFA) administration. Our studies show significant increase in CGRP expression in female mice, comparing to male mice. Furthermore, we demonstrate, that activation of trigeminal nociceptors by peripheral inflammation causes significant increase in expression of IL-1B, IL-6, TNF and BDNF in male mice, comparing to female mice. This phenomenon may be involved in clinically observed gender-dependent differences in the frequency of both migraine and other trigeminal nerve-related facial pain disorders.
BDNF modifies hippocampal KCC2 and NKCC1 expression in a temporal lobe epilepsy model
Authors: Eftekhari Sanaz , Mehrabi Soraya , Soleimani Mansooreh , Hassanzadeh Gholamreza , Shahrokhi Amene , Mostafavi Hossein , Hayat Parisa , Barati Mahmood , Mehdizadeh Hajar , Rahmanzadeh Reza , Hadjighassem Mahmoud , Joghataei Mohammad
Published: November 2023

Excitatory GABA actions, induced by altered expression of chloride transporters (KCC2/NKCC1), can contribute to seizure generation in temporal lobe epilepsy. In the present study, we evaluated whether BDNF administration can affect KCC2/NKCC1 expression, ictogenesis and behavioral alterations in this paradigm. Status epilepticus was induced in male rats with pilocarpine, followed by a treatment of either a single high dose or multiple injections of BDNF during the latent phase of temporal lobe epilepsy. Chloride transporters expression, spontaneous recurrent seizures, and hyperexcitability post-seizural behaviors were evaluated after treatment. NKCC1 protein expression was markedly upregulated, whereas that of KCC2 was significantly downregulated in epileptic hippocampi compared to intact controls. Application of BDNF (both single high dose and multiple injections) increased KCC2 expression in epileptic hippocampi, while NKCC1 expression was downregulated exclusively by the single high dose injection of BDNF. Development of spontaneous recurrent seizures was delayed but not prevented by the treatment, and hyperexcitability behaviors were ameliorated for a short period of time. To prevent GABA-A mediated depolarization and design appropriate treatment strategies for temporal lobe epilepsy, chloride transporters can be considered as a target. Future studies are warranted to investigate any possible therapeutic effects of BDNF via altering chloride transporters expression.
Expression and localization of cannabinoid receptor 1 in rats’ brain treated with acute and repeated morphine
Authors: Jin Lingjing , Pan Lizhen , Guo Yan , Zheng Yuguo , Nie Zhiyu , Zhu Rongrong
Published: November 2023

Morphine induces adaptive changes in gene expression throughout the reward circuitry of brain. Recent research has proven the functional interactions between opioid and endogenous cannabinoid system in the central nervous system (CNS). The cannabinoid receptor 1 (CB1-R) is one of the receptors that mediate the actions of cannabinoids and endocannabinoids in the CNS. Here, we investigated the expression of CB1-R in mRNA and protein levels in the brains of rats treated with acute and repeated morphine. Three groups of rats received intraperitoneal injections (ip injections) of saline, acute morphine (10 mg/kg) and repeated morphine (10 mg/kg, twice daily for 12 consecutive days), and the mRNA levels and protein expressions of CB1-R were examined. RT-PCR and western blot analyses supported that both mRNA and protein levels of CB1-R in cortex, cerebellum and hippocampus were increased by repeated morphine treatment. However, the mRNA level in cerebellum was down-regulated only after acute morphine treatment and would returned to basal levels later. We used immunohistochemistry techniques to determine the functional expression of CB1-R in morphine treated rat’s brain. Enzyme-Linked Immunosorbent Assay (ELISA) revealed the significant increase of cytokine (IL-1beta, IL-6) levels in the repeated morphine treatment rats’ cortex and hippocampus regions, which are both addiction-related brain areas. In addition, the results from RT-PCR and western blot assay indicated that the expression of CB1-R was directly increased by morphine treatment in vitro. All the results indicated that the CB1-R expression could be changed by morphine exposure and it might be involved in neural immune function, which provided a potential target for neurogenic disease treatment.
The Relationship between Body Mass Index (BMI) and Clinical Pathological Profile of Invasive Breast Carcinoma Patients in the Department of Anatomic Pathology FKUI/RSCM from 2019 to 2022
Authors: Yournita Debbie , Rustamadji Primariadewi
Published: November 2023

Background

Breast cancer is the most prevalent malignancy among women and is the second largest contributor to cancer-related deaths globally. Women with a high body mass index (BMI) are at a greater risk of developing breast cancer and an increased risk of lymph node metastasis. This retrospective study aimed to determine the relationship between BMI and the clinical pathological profile of invasive breast carcinoma (IBC) patients.

Method

This study was conducted from 2019 to 2022. Data were collected through the archives of the Department of Anatomic Pathology FKUI/RSCM (Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital). Clinical pathological data were collected. The histological type, grade, lymphovascular invasion, and lymph node metastasis of the tumors were re-evaluated. The chi-square hypothesis test was used to determine the relationship between BMI and clinical pathological profile. The results were considered statistically significant with a p <0.05.

Result

A total of 151 cases of IBC with lymph node dissection from 2019 to 2022 were included in this study, all of which were female, with the majority being >50 years old (55%). The most common tumor size and histological type were T2 (39,7%) and invasive ductal carcinoma NST (74,8%), 49% of cases were grade 3 tumors. Most IBC cases had an obese BMI (49%), 63.1% with lymph node metastasis had an overweight-obese BMI. Statistical analysis was performed between BMI and lymph node metastasis status, and p-value=0.025 was obtained. The relationship between tumor size and lymphovascular invasion with lymph node metastasis was also found to be statistically significant, as indicated by a p-value of 0.000.

Conclusion

Cases with lymph node metastasis had the highest rate of overweight-obesity, and a significant relationship was found between BMI and lymph node metastasis status. A significant relationship was also found between tumor size and lymphovascular invasion with lymph node metastasis.
Inovações tecnológicas e capacidades dinâmicas: estudo de caso em cooperativas de eletricidade
Authors: Cardoso João Vanio Mendonça , Camilo Sílvio Parodi Oliveira , Picolo Jaime Dagostim
Published: November 2023

Com o avanço e a difusão das inovações tecnológicas, vem se exigindo das organizações o desenvolvimento de modelos de negócios intensivos e dependentes de informação e conhecimento. Sendo assim, é importante que as organizações busquem constantemente, explorar e implementar novas oportunidades dos ambientes externo e interno. Esse é justamente o foco das capacidades dinâmicas, que identificam por varredura, busca e exploração de novas ideias que atendam ao ambiente em mutação. Esta pesquisa tem como objetivo verificar a contribuição das capacidades dinâmicas para a inovação, a partir da lente dos microfundamentos e as principais tendências tecnológicas para o futuro nas cooperativas de eletricidade. Com vistas a atender o objetivo proposto, a pesquisa teve uma abordagem qualitativa, se caracteriza como exploratória e quanto aos procedimentos técnicos, trata-se de um estudo multicascos. Os dados foram coletados por entrevistas semiestruturada e analisados por meio da análise de conteúdo qualitativa. Como resultados alcançados, foi possível constatar que as capacidades dinâmicas e os seus microfundamentos contribuem para a inovação por meio da análise de como as cooperativas de eletricidade: identificam, aproveitam e reconfiguram as oportunidades das inovações tecnológicas.
Identificação dos resíduos sólidos e líquidos oriundos da produção de café e seus potenciais usos: uma revisão
Authors: Lopes Isac , De Oliveira Gabriel Henrique Horta , De Oliveira Ana Paula Lelis Rodrigues , Da Silva Diego Mathias Natal
Published: November 2023

O Brasil é o maior produtor e exportador de café do mundo, e uma das consequências dessa vasta produção é o volume elevado de resíduos gerados durante a produção do café. Mesmo depois de cerca de 150 anos de história da cafeicultura no país, ainda são escassas as pesquisas e levantamentos de dados acerca da geração de tais resíduos, uma vez que, o foco das pesquisas nacionais tem maior direcionamento para outros temas relacionados à cafeicultura. Nesse sentido, o presente trabalho tem como objetivo identificar e analisar artigos científicos, publicados nos anos de 2006 a 2022 em periódicos indexados, com temática relacionada aos impactos gerados no meio ambiente pelos resíduos físicos e químicos oriundos das fases de implantação e condução, colheita e pós-colheita do café. Sendo este estudo realizado por meio de revisão sistemática da literatura e revisão integrativa de literatura. Foram selecionados dezenove artigos, utilizando as seguintes palavras-chave em português e suas respectivas traduções para a língua inglesa: água residuária (wastewater); cafeicultura (coffee planting); compostagem (composting); compostos químicos (chemical composts); resíduos líquidos (liquid residues); resíduos sólidos (solid residues); resíduos vegetais (crop residues); viabilidade econômica (economic viability); e sustentabilidade (sustainability).
Características produtivas de capins tropicais com e sem irrigação
Authors: Diallo Mamadou Cellou Abdoulaye , Gomes Eder Pereira , Da Silva Mábio Silvan José , Sanches Arthur Carniato , Santos Rodrigo Couto , Mello Kamila de Almeida Monaco , Bezerra Leandro Cardoso , De Souza Lariça Alves
Published: November 2023

A distribuição irregular das chuvas e as baixas temperaturas no inverno costumam limitar a produtividade das forrageiras tropicais. A irrigação pode ser utilizada para atenuar estas adversidades climáticas. Assim, esta pesquisa foi implantada com o objetivo de avaliar as características produtivas (produtividade, composição botânica e sazonalidade de produção) dos principais gêneros de forrageiras tropicais (Megathyrsus maximus cv. Mombaça, Urochloa Brizantha cv. MG-5 e Cynodon spp cv. Tifton 85), com e sem irrigação, no período de 20 fevereiro 2020 a 23 fevereiro 2021. O experimento foi realizado na Área Experimental de Irrigação da Universidade Federal da Grande Dourados. O delineamento experimental utilizado foi de blocos casualizados em esquema de parcelas subdivididas, com quatro repetições. As parcelas foram estabelecidas com e sem irrigação e as subparcelas formadas pelos capins. Os ciclos de coleta ocorreram a cada 28 dias, avaliando-se: produtividade, percentagem de material morto, relação folha/colmo e relação entressafra/safra. Todos os capins apresentaram produtividade de matéria seca maior com irrigação, obtendo os seguintes valores: 124,4 kg ha-1dia-1 para o capim MG-5, 135,1 kg ha-1dia-1 para o capim Mombaça e 98,8 kg ha-1dia-1 para o capim Tifton 85. A irrigação teve efeitos diferentes sobre a relação folha/colmo e porcentagem de material morto dos capins. A relação entressafra/safra foi maior com irrigação para todos os capins, destacando-se o Tifton 85 com valor igual a 0,83.
Copyright as Credit Collateral in Legal Perspective: Evidence from Indonesia
Authors: Rohendi Acep , Kharisma Dona Budi , Purwadhi  , Putranto Muqtadir Ghani
Published: November 2023

Objective: There are two main points in this legal research: (1) to analyze the legal problem of copyright as credit collateral in Indonesia (2) and to compare copyright law arrangement in South Korea.
Method: This study uses normative legal research. The legal documents used are South Korean and Indonesian copyright laws and regulations. Utilizing techniques from literature studies, the procedure of gathering legal materials is carried out.
Results: There are still issues with the regulation and application of the law in Indonesia, particularly in relation to the issue of valuation and appraisal for the liquidation of intellectual property law (hereafter referred to Indonesia language as HKI) because there isn't a secondary market, the legal infrastructure mechanism for executing HKI is unclear.
Conclusions: In contrast to Indonesia, South Korea itself has rules and regulations for copyright assessment that have been supported by the South Korean Government, such as having a special panel of judges that exclusively handles HKI disputes, copyright collection agencies in South Korea have trusteeship agreement with copyright holders so that their copyrights are transferred to copyright collection agencies as trust property. This legal problem occurs because the implementation of regulations in Indonesia still overlaps, so that the implementing rules are still not optimal.
KHÔNG GIAN VĂN HÓA VẠN LẠI – YÊN TRƯỜNG
Authors: Đỗ Thanh Mai , Lê Thị Thảo
Published: November 2023

Mặc dù có vai trò quan trọng đối với cục diện Nam - Bắc triều ở thế kỷ XVI nhưng trong công trình nghiên cứu của các tác giả Việt Nam thời cận hiện đại ít đề cập đến kinh đô Vạn Lại - Yên Trường. Đến nay, kinh đô kháng chiến của triều đại Lê Trung hưng cũng chỉ còn tồn tại dưới dạng phế tích, nhưng không gian văn hóa Vạn Lại - Yên Trường vẫn lưu giữ được nhiều giá trị văn hóa độc đáo. Kinh đô được chuyển qua lại nhiều lần giữa hai khu vực Vạn Lại và Yên Trường, cách nhau chỉ khoảng 20 km. Bài viết khái quát về không gian tồn tại của kinh đô này và những giá trị văn hóa vật thể, phi vật thể còn sót lại.
PHƯƠNG THỨC LẠ HÓA NGÔN NGỮ CỦA NHÀ VĂN MẠC NGÔN TRONG TIỂU THUYẾT “BÁU VẬT CỦA ĐỜI”
Authors: Tạ Thị Thủy
Published: November 2023

Giải Nobel văn học danh giá năm 2012 đã gọi tên Mạc Ngôn - nhà văn Trung Quốc, các tác phẩm của ông đã được dịch ra nhiều thứ tiếng và được thế giới đánh giá cao. Trong sự nghiệp sáng tác của nhà văn, không thể không nhắc tới tác phẩm “Báu vật của đời” được xem là đỉnh cao của nghệ thuật ngôn từ. Đặc biệt, qua tìm hiểu tác phẩm, chúng tôi nhận thấy phương thức lạ hóa ngôn ngữ là tiêu biểu nhất, tạo nên sự phá cách độc đáo trong tiểu thuyết “Báu vật của đời”.
NGHIÊN CỨU THỰC TRẠNG SỬ DỤNG TIẾNG ĐỊA PHƯƠNG CỦA SINH VIÊN NGÀNH GIÁO DỤC MẦM NON TẠI TRƯỜNG ĐẠI HỌC VĂN HÓA, THỂ THAO VÀ DU LỊCH THANH HÓA
Authors: Mai Anh Vũ Vũ , Lê Thị Hương
Published: November 2023

Sinh viên ngành Giáo dục mầm non quyết định đến chất lượng nguồn nhân lực nhà giáo trong tương lai trong việc nuôi dưỡng, chăm sóc và giáo dục trẻ. Những năm qua, sinh viên ngành Giáo dục mầm non Trường Đại học Văn hóa, Thể thao và Du lịch Thanh Hóa đã có sự phát triển cả về số lượng và chất lượng. Tuy nhiên, đào tạo sinh viên ngành Giáo dục mầm non vẫn đang đối diện nhiều khó khăn. Việc sinh viên còn sử dụng tiếng địa phương trong giao tiếp và học tập là vấn đề đáng bàn. Bài viết đề cập đến việc nói tiếng địa phương của sinh viên ngành Giáo dục mầm non hệ đại học chính quy tại Trường Đại học Văn hóa, Thể thao và Du lịch Thanh Hóa. Nhóm tác giả tập trung xây dựng phiếu hỏi điều tra về thực trạng nói tiếng địa phương của sinh viên các lớp năm 1,2,3 và năm cuối, sau đó tiến hành thu thập dữ liệu và phân tích trên SPSS. Kết quả nghiên cứu trên 171 đối tượng đã chỉ ra một số nguyên nhân, từ đó nhóm tác giả đề xuất những giải pháp nhằm đáp ứng yêu cầu của ngành Giáo dục mầm non hiện nay.
A MATEMÁTICA E A INTERDISCIPLINARIDADE: PERSPECTIVAS PARA A APRENDIZAGEM DOS ESTUDANTES DO ENSINO FUNDAMENTAL DE ACORDO COM DOCUMENTOS OFICIAIS DA EDUCAÇÃO
Authors: Pereira Magno Andris Costa , Almeida Joemilia Maria Pinheiro , Costa Manoel dos Santos , Silva Marcia Valéria Melo e
Published: November 2023

O presente artigo tem por objetivo fazer uma reflexão acerca do ensino de Matemática nos anos finais do Ensino Fundamental sob a perspectiva da interdisciplinaridade, de acordo com as recomendações de documentos oficiais da Educação. Trata-se, portanto, de uma pesquisa bibliográfica, de natureza qualitativa, cujo objeto de análise foi o arcabouço documental que inclui, dentre outros, documentos oficiais. As reflexões apresentadas no presente artigo nos levam a pensar na importância do currículo escolar e na necessidade de buscar novas perspectivas para o ensino, em sua concepção e em sua reforma. O Ensino Fundamental, etapa obrigatória a todos os cidadãos, deve levar em consideração a realidade social e as características culturais de nossa sociedade, e aproveitar todos os meios de que hoje se dispõe. Por isso, a organização do currículo e o desenvolvimento dos conteúdos, de forma fragmentada/isolada, em sala de aula, eliminam a possibilidade do exercício da produção interdisciplinar no processo de ensino-aprendizagem.