bluuebunny/crossref_metadata_2025 huggingface dataset:
Revealing the Materials, Painting Techniques, and State of Preservation of a Heavily Altered Early 19th Century Greek Icon through MA-XRF
Authors: Mastrotheodoros Georgios P. , Asvestas Anastasios , Gerodimos Theofanis , Anagnostopoulos Dimitrios F.
Published: February 2023

Macroscopic X-ray fluorescence analysis (MA-XRF) is a non-destructive analytical technique that allows for the rapid and thorough investigation of paintings; therefore, it is nowadays increasingly involved in relevant studies. In the present work, a state-of-the-art MA-XRF set-up is utilized to identify the painting materials and techniques and document the state of preservation of an early 19th-century AD Greek religious panel painting (“icon”). The artifact in consideration has received extensive restoration interventions in the past and is considerably decayed; for these reasons, the interpretation of the relevant MA-XRF elemental intensity distribution maps is challenging. In this framework, it is demonstrated how the elemental distribution maps can be explored and interpreted to lead to a thorough investigation of the painting in consideration. In particular, the MA-XRF data interpretation allowed for the identification of the original palette that includes the rather rarely employed–in icon painting-yellow lead-antimonate pigment, led to the spotting of an invisible inscription, to the documentation of the preparation/gesso layer and the preliminary drawing. Additionally, it was possible to discriminate between the original painting and the later interventions, while the collected data revealed that the painting originally had two cross-bars attached to its back. Ultimately, the presented case study can be utilized as a guide for the proper interpretation of MA-XRF data from decayed and altered icons.
Social Bots’ Role in the COVID-19 Pandemic Discussion on Twitter
Authors: Zhang Yaming , Song Wenjie , Shao Jiang , Abbas Majed , Zhang Jiaqi , Koura Yaya H. , Su Yanyuan
Published: February 2023

Social bots have already infiltrated social media platforms, such as Twitter, Facebook, and so on. Exploring the role of social bots in discussions of the COVID-19 pandemic, as well as comparing the behavioral differences between social bots and humans, is an important foundation for studying public health opinion dissemination. We collected data on Twitter and used Botometer to classify users into social bots and humans. Machine learning methods were used to analyze the characteristics of topic semantics, sentiment attributes, dissemination intentions, and interaction patterns of humans and social bots. The results show that 22% of these accounts were social bots, while 78% were humans, and there are significant differences in the behavioral characteristics between them. Social bots are more concerned with the topics of public health news than humans are with individual health and daily lives. More than 85% of bots’ tweets are liked, and they have a large number of followers and friends, which means they have influence on internet users’ perceptions about disease transmission and public health. In addition, social bots, located mainly in Europe and America countries, create an “authoritative” image by posting a lot of news, which in turn gains more attention and has a significant effect on humans. The findings contribute to understanding the behavioral patterns of new technologies such as social bots and their role in the dissemination of public health information.
Comparison of eHealth Literacy Scale (eHEALS) and Digital Health Literacy Instrument (DHLI) in Assessing Electronic Health Literacy in Chinese Older Adults: A Mixed-Methods Approach
Authors: Xie Luyao , Mo Phoenix K. H.
Published: February 2023

This study compared the reliability, construct validity, and respondents’ preference of the Chinese version of 8-item eHEALS (C-eHEALS) and 21-item DHLI (C-DHLI) in assessing older adults’ electronic health (eHealth) literacy using a mixed-methods approach. A web-based, cross-sectional survey was conducted among 277 Chinese older adults from September to October 2021, and 15 respondents were subsequently interviewed to understand their preference of scale to use in practice. Results showed that the internal consistency and test-retest reliability of both scales were satisfactory. For the construct validity, the C-DHLI score showed stronger positive correlations with having Internet use for health information and higher educational attainments, occupational skill levels, self-rated Internet skills, and health literacy than the C-eHEALS score. In addition, younger age, higher household income, urban residence, and longer Internet use history were only positively correlated with C-DHLI score. Qualitative data suggested that most interviewees perceived the C-DHLI as more readable than C-eHEALS for its clear structure, specific description, short sentence length, and less semantic complexity. Findings revealed that both scales are reliable tools to measure eHealth literacy among Chinese older adults, and the C-DHLI seemed to be a more valid and favored instrument for the general Chinese older population based on the quantitative and qualitative results.
Variable Pacing Is Associated with Performance during the OCC® Ultra-Trail du Mont-Blanc® (2017–2021)
Authors: Corbí-Santamaría Pedro , Herrero-Molleda Alba , García-López Juan , Boullosa Daniel , García-Tormo Vicente
Published: February 2023

The current evidence suggests that pacing may not be affected by performance level or sex in trail-running races as may occur in road running races. However, the previous studies included races of >100 km. Therefore, we aimed to verify the influence of performance level and sex on pacing in the last four (2017, 2018, 2019, and 2021) editions of a shorter (56.3 km) ultra-trail running race (i.e., Orsières–Champex–Chamonix; OCC®) that maintained the same race profile. The mean finishing time for the 5656 participants was 10 h 20 min 33 s ± 2 h 01 min 19 s. Pacing variability (CV%) was higher in high-level participants, thus showing a greater ability to adapt their pace to the race profile than low-level runners. Males also had a higher pacing variability than females although the effect sizes were small. Based on the current findings, we may recommend for non-elite OCC® participants to adapt their pace to the race profile with a slower pace during uphills and a faster pace during downhills. Further studies including participants’ experience are necessary to confirm the effectiveness of this suggestion in trail-running races of variable distances.
Understanding Unmet Care Needs of Rural Older Adults with Chronic Health Conditions: A Qualitative Study
Authors: Asante Dennis , McLachlan Craig S. , Pickles David , Isaac Vivian
Published: February 2023

Background: Rural populations experience poorer access to the necessary health services for chronic health conditions. Although studies of rural healthcare access continue to expand, most are based on quantitative data, yet normative views and lived experiences of rural adults might offer a better understanding of healthcare access and their specific unmet needs. This qualitative study sought the views of both rural-centric older people and healthcare professionals to understand health needs, barriers, and enablers of accessing health services, with a focus on chronic health condition(s). Methods: Between April and July 2022, separate in-depth interviews were conducted with 20 older people (≥60 years) in a rural South Australian community. Additionally, focus group interviews were conducted with 15 healthcare professionals involved in providing health services to older adults. Transcripts were coded using the NVivo software and data were thematically analysed. Results: Participants described a range of unmet care needs including chronic disease management, specialist care, psychological distress, and the need for formal care services. Four barriers to meeting care needs were identified: Workforce shortages, a lack of continuity of care, self-transportation, and long waiting times for appointments. Self-efficacy, social support, and positive provider attitudes emerged as crucial enabling factors of service use among rural ageing populations. Discussion: Older adults confront four broad ranges of unmet needs: Chronic disease management care, specialist care, psychological care, and formal care. There are potential facilitators, such as self-efficacy, provider positive attitudes, and social support, that could be leveraged to improve healthcare services access for older adults.
Bilateral Transfer of Performance between Real and Non-Immersive Virtual Environments in Post-Stroke Individuals: A Cross-Sectional Study
Authors: Mota Deise M. S. , Moraes Íbis A. P. , Papa Denise C. R. , Fernani Deborah C. G. L. , Almeida Caroline S. , Tezza Maria H. S. , Dantas Maria T. A. P. , Fernandes Susi M. S. , Ré Alessandro H. N. , Silva Talita D. , Monteiro Carlos B. M.
Published: February 2023

(1) Background: Post-stroke presents motor function deficits, and one interesting possibility for practicing skills is the concept of bilateral transfer. Additionally, there is evidence that the use of virtual reality is beneficial in improving upper limb function. We aimed to evaluate the transfer of motor performance of post-stroke and control groups in two different environments (real and virtual), as well as bilateral transfer, by changing the practice between paretic and non-paretic upper limbs. (2) Methods: We used a coincident timing task with a virtual (Kinect) or a real device (touch screen) in post-stroke and control groups; both groups practiced with bilateral transference. (3) Results: Were included 136 participants, 82 post-stroke and 54 controls. The control group presented better performance during most parts of the protocol; however, it was more evident when compared with the post-stroke paretic upper limb. We found bilateral transference mainly in Practice 2, with the paretic upper limb using the real interface method (touch screen), but only after Practice 1 with the virtual interface (Kinect), using the non-paretic upper limb. (4) Conclusions: The task with the greatest motor and cognitive demand (virtual—Kinect) provided transfer into the real interface, and bilateral transfer was observed in individuals post-stroke. However, this is more strongly observed when the virtual task was performed using the non-paretic upper limb first.
Esophageal and Head and Neck Cancer Patients Attending Ocean Road Cancer Institute in Tanzania from 2019 to 2021: An Observational Study
Authors: Mwelange Luco P. , Mamuya Simon H. D. , Mwaiselage Julius , Bråtveit Magne , Moen Bente E.
Published: February 2023

Background: Cancer in Africa is an emerging public health problem that needs urgent preventive measures, particularly in workplaces where exposure to carcinogens may occur. In Tanzania, the incidence rate of cancer and mortality rates due to cancers are increasing, with approximately 50,000 new cases each year. This is estimated to double by 2030. Methods: Our hospital-based cross-sectional study describes the characteristics of newly diagnosed patients with head and neck or esophageal cancer from the Ocean Road Cancer Institute (ORCI), Tanzania. We used an ORCI electronic system to extract secondary data for these patients. Results: According to the cancer registration, there were 611 head and neck and 975 esophageal cancers recorded in 2019–2021. Two-thirds of these cancer patients were male. About 25% of the cancer patients used tobacco and alcohol, and over 50% were involved in agriculture. Conclusion: Descriptions of 1586 head and neck cancer patients and esophageal cancer patients enrolled in a cancer hospital in Tanzania are given. The information may be important for designing future studies of these cancers and may be of value in the development of cancer prevention measures.
Health, Hope, and Harmony: A Systematic Review of the Determinants of Happiness across Cultures and Countries
Authors: Singh Sunitha , Kshtriya Sowmya , Valk Reimara
Published: February 2023

The aim of this study was to review the literature on what constitutes happiness across cultures and countries to advance scholarly knowledge on the happiness construct. A systematic review was conducted to examine the determinants of happiness in samples across cultures and countries. Five different databases, including APA PsycNet, EBSCO-Academic, EBSCO-Business, Project MUSE, and Google Scholar, grey literature, and in-text references from relevant review articles were used. A total of 155 articles were included in the review, encompassing studies from over 100 countries and 44 cultures. Myriad determinants of happiness were found that were placed into three broad categories labeled Health, Hope, and Harmony. The predominant happiness determinants were mental, emotional, and physical well-being, a purposeful holistic work–life balance, nurturing social relationships, caring for self and others, and being in harmony with one’s culture, traditions, community, religion, and environment. This study engendered an “Integrated Model of the Determinants of Happiness” to provide a universally applicable conceptualization of the happiness construct. By examining studies on determinants of happiness across the globe in the past 90 years, this review uncovered that happiness constitutes multiple determinants that fall under three major categories: ‘Health’, ‘Hope’, and ‘Harmony’.
Ergonomics of E-Learning Workstations and the Prevalence of Musculoskeletal Disorders—Study among University Students
Authors: Janc Magdalena , Jozwiak Zbigniew , Jankowska Agnieszka , Makowiec-Dabrowska Teresa , Kujawa Jolanta , Polanska Kinga
Published: February 2023

The prevalence of musculoskeletal disorders (MSD) has increased significantly in recent years. The COVID-19 pandemic has led to a fundamental change in the lifestyles, ways of learning and working patterns of the general population, which in turn, might lead to health consequences. The aim of this study was to evaluate the conditions of e-learning and the impact of the learning modality on the occurrence of musculoskeletal symptoms among university students in Poland. This cross-sectional study included 914 students who completed an anonymous questionnaire. The questions covered two time periods (before and during the COVID-19 pandemic) and were aimed at obtaining information about lifestyle (including physical activity using the modified International Physical Activity Questionnaire, 2007 (IPAQ), perceived stress and sleep patterns), the ergonomics of computer workstations (by Rapid Office Strain Assessment, 2012 (ROSA) method), the incidence and severity of musculoskeletal symptoms (by the Nordic Musculoskeletal Questionnaire, 2018 (NMQ)) and headaches. The main differences between the two periods were statistically significant according to the Wilcoxon test in terms of physical activity, computer use time, and severity of headaches. During the COVID-19 pandemic, there was a significant increase in MSD (68.2% vs. 74.6%) and their intensity (2.83 ± 2.36 vs. 3.50 ± 2.79 points) among the student population (p < 0.001). In the group of students with MSD, there was a high musculoskeletal load, due to the lack of ergonomic remote learning workstations. In future, a thorough study should be carried out, and there is an urgent need to raise students’ awareness of arranging learning workstations according to ergonomic principles in order to prevent the occurrence of musculoskeletal problems.
The Transition to Adulthood in Children of Depressed Parents: Long-Term Follow-Up Data from the Family Talk Preventive Intervention Project
Authors: Myers Taylor L. , Gladstone Tracy R. G. , Beardslee William R.
Published: February 2023

Little is known about the effects of parental depression on offspring as they transition to adulthood—a challenging time developmentally, when late adolescents must separate from home, achieve intimate relationships, and develop a sense of identity. We present long-term quantitative and qualitative data from early adolescents with a depressed parent who were randomized to one of two family-based preventive interventions and followed over time, across the transition to young adulthood. Specifically, we present clinical measures of psychopathology and Likert-scale questionnaire data from young adults and their parents regarding the transition to adulthood and perceptions of the interventions. We also report in-depth qualitative interview data from young adults about the effects of parental depression on their transition to adulthood. Findings suggest that leaving home, establishing relationships, and coping with stressors may be challenging for emerging adults. Furthermore, the interviews highlight the importance of siblings, the burden of parental depression, and the development of self-understanding and empathy in young adults who grew up with a depressed parent. Data suggest that clinicians, policy makers, educators, and employers must address the preventive and clinical needs of young people and their families as they transition to young adulthood after growing up with depressed parents.
A Qualitative Study on Young Men’s Experiences of Intentional Weight-Gain
Authors: Donnachie Craig , Sweeting Helen , Hunt Kate
Published: February 2023

This qualitative study investigated how young men perceive their body image and experiences of purposively gaining weight, and what these reveal about broader sociocultural meanings around food, consumption and male body image. The participants in this study were a subsample of men participating in the ‘GlasVEGAS’ study which examined the effect of weight-gain and weight loss on metabolism, fitness and disease risk in young adult men. Twenty-three qualitative, semi-structured interviews were conducted with thirteen men (mean age 23 years) at GlasVEGAS baseline (n = 10) and weight-gain (6-week) follow-up assessment (n = 13). Data were analysed using the principles of framework analysis. The majority of men viewed the foods provided as part of the GlasVEGAS study as ‘luxury’ items despite their being of low nutritional value. The weight-gain process prompted men to reflect on how cultural norms and social environments may amplify overeating. Several described being surprised at how quickly they assimilated unhealthy eating habits and/or gained weight. Some valued changes in their appearance associated with weight-gain, including appearing larger or having increased muscle size. These factors are vital to consider when developing weight management initiatives targeting young men, including the valorisation of unhealthy foods, wider social influences on diet and male body image ideals.
Effect of a One-Time Communication Training Session on Dental Students’ Self-Efficacy Expectancy
Authors: Grötz Thekla J. , Zöll Lisa , Hollinderbäumer Anke , Nowak Thomas , Zajontz Tamara , Behling Lina , Fischbeck Sabine , Schmidt Uwe , Deschner James
Published: February 2023

An implementation of training units that provide evidence for improving students’ communication skills in the dental curriculum is now more than ever of utmost importance. This study aimed to investigate how students assess their skills after communication training and whether this training also increased students’ self-efficacy expectancy. A total of 32 male and 71 female students with a mean age of 25.6 ± 3.9 years participated in the study. Self-assessment of communication skills and self-efficacy expectancies were collected at two time points using Likert scales. Our study shows that the communication training, consisting of a practical exercise with actors and an online theory module, significantly improved the students’ self-assessment of their communication skills and also improved some aspects of self-efficacy expectancy. These results indicate that, in addition to the practical and technical-theoretical training of students, communication training is essential in the dental curriculum. In summary, this study showed that a one-time practical exercise with actors together with an online theory module could improve both the self-assessment of communication competence and some aspects of self-efficacy expectancy, which demonstrates the importance of training communication skills alongside practical and technical-theoretical training.
Assessing Decentering Capacity in Athletes: A Moderated Mediation Model
Authors: Diotaiuti Pierluigi , Valente Giuseppe , Corrado Stefano , Mancone Stefania
Published: February 2023

Decentering has received more attention in sports literature as a self-regulating skill capable of significantly limiting episodes of mental block in competitive situations. This contribution depicts a comparative study conducted with 375 Italian national and international athletes. The objective was to evaluate athletes’ decentralization skills across different sports and levels of competition and test a mediation model of decentering in sports with coping and emotional balance variables. Pearson bivariate correlations, linear hierarchical regression, and simple mediation analysis were conducted for all main measures (The Decentering Sport Scale, The Emotion Regulation Questionnaire, and The Coping Orientations to Problems Experienced). Outputs reported significant associations with emotional regulation and coping styles. Mediation analysis confirmed the central mediating role of decentering capacity which has indirect effects on both the coping ability of problem solving (z-value = 2.986; p = 0.003) and cognitive reappraisal (z-value = 2.779; p = 0.005). Decentering acts as a mediator between an athlete’s positive attitude, problem-orientation ability, and management of emotions in competition through cognitive reappraisal. The study highlights the significance of evaluating and enhancing decentralization skills in order to establish specific action mechanisms, which are crucial for both peak performance and the athlete’s health.
The Influence of Presentation Frames of Visualization Information for Safety on Situational Awareness under a Three-Level User-Interface Design
Authors: Yuan Xiaofang , Yan Jing , Sun Linhui , Cheng Fangming , Guo Zigu , Yu Hongzhi
Published: February 2023

To explore the influence of the construction and presentation frames of visualization information for safety (VIS) on people’s situation awareness (SA), we designed a three-level user interface (UI) of VIS based on the three-stage SA theory, including perception (SA1), comprehension (SA2), and projection (SA3). Then, 166 subjects were recruited and divided into three groups to participate in the experiment, in which SA was measured by the situation-present-assessment method (SPAM) and situation-awareness-rating technique (SART), and eye-movement data were recorded. The results show that the level−3 UI design could effectively improve the subjects’ SA levels. Although the increase in VIS displayed caused by the higher UI level led to a decrease in the perception-stage score of SA, the level−3 UI fully considered the three stages of human information processing, and helped improve the SA of the subjects; the overall SA score measured using the SART method was not significant, but the result was consistent with the SPAM. There was a framing effect on the presentation of VIS, and subjects perceived different degrees of risk under different presentation frames; that is, less risk under the positive frame, more risk under the negative frame, and a higher level of SA under the positive frame compared with the negative frame. To some extent, the nearest-neighbor-index (NNI) algorithm could be utilized to quantify subjects’ eye-tracking fixation mode. While the frames were guided by the high-level interface and the positive presentation frame, the distribution of the subjects’ gaze points was more discrete; they could grasp the relevant information more comprehensively and had a relatively high level of SA. To some extent, this study can provide a reference for the design and optimization of the VIS presentation interface.
Effect of Socioeconomic Status on Altruistic Behavior in Chinese Middle School Students: Mediating Role of Empathy
Authors: Liu Xiaomin , Zhang Yuqing , Chen Zihao , Xiang Guangcan , Miao Hualing , Guo Cheng
Published: February 2023

Previous studies have shown that socioeconomic status is correlated to altruistic behavior. The role of empathy as one of the motivations for altruistic behavior is gradually gaining attention among researchers. This study explores the role of empathy in the mechanisms of socioeconomic status and altruistic behavior in Chinese adolescents. A total of 253 middle school students from Northern China participated in this study, which included the dictator game and Interpersonal Relation Index. Results showed that (1) low-SES students behaved more generously than high-SES students; (2) the students were more generous to the low-SES recipients, as shown when offering them more money in the dictator game; (3) affective rather than cognitive empathy mediates the relationship between socioeconomic status and altruistic behavior. The findings provide evidence for the validation of the empathy–altruism hypothesis in a group of Chinese adolescents. Meanwhile, it reveals the path to improving altruistic behavior through the promotion of empathy, especially for individuals of high socioeconomic status.
Urbanization-Related Environmental Factors and Hemorrhagic Fever with Renal Syndrome: A Review Based on Studies Taken in China
Authors: Li Shujuan , Zhu Lingli , Zhang Lidan , Zhang Guoyan , Ren Hongyan , Lu Liang
Published: February 2023

Hemorrhagic fever with renal syndrome (HFRS) is a rodent-borne disease that has threatened Chinese residents for nearly a century. Although comprehensive prevent and control measures were taken, the HFRS epidemic in China presents a rebounding trend in some areas. Urbanization is considered as an important influencing factor for the HFRS epidemic in recent years; however, the relevant research has not been systematically summarized. This review aims to summarize urbanization-related environmental factors and the HFRS epidemic in China and provide an overview of research perspectives. The literature review was conducted following the PRISMA protocol. Journal articles on the HFRS epidemic in both English and Chinese published before 30 June 2022 were identified from PubMed, Web of Science, and Chinese National Knowledge Infrastructure (CNKI). Inclusion criteria were defined as studies providing information on urbanization-related environmental factors and the HFRS epidemic. A total of 38 studies were included in the review. Changes brought by urbanization on population, economic development, land use, and vaccination program were found to be significantly correlated with the HFRS epidemic. By changing the ecological niche of humans—affecting the rodent population, its virus-carrying rate, and the contact opportunity and susceptibility of populations—urbanization poses a biphasic effect on the HFRS epidemic. Future studies require systematic research framework, comprehensive data sources, and effective methods and models.
Beyond the Hype: (How) Are Work Regimes Associated with Job Burnout?
Authors: du Bois Kristen , Sterkens Philippe , Lippens Louis , Baert Stijn , Derous Eva
Published: February 2023

Job burnout affects countless workers and constitutes a major issue in working life. Prevention strategies such as offering part-time options and shorter working weeks have been widely advocated to address this issue. However, the relationship between shorter work regimes and burnout risk has not yet been investigated across diverse working populations applying validated measures and frameworks for job burnout. Building on the most recent operationalisation of job burnout and the seminal job demands–resources theory, the purpose of the current study is to investigate whether shorter work regimes are associated with lower burnout risk and whether the job demands–resources explain this association. To this end, a heterogenous sample of 1006 employees representative for age and gender completed the Burnout Assessment Tool (BAT) and Workplace Stressors Assessment Questionnaire (WSAQ). Our mediation analyses yield a very small but significant indirect association between work regimes and burnout risk through job demands, but no significant total or direct association between work regimes and burnout risk. Our result suggests that employees in shorter work regimes experience slightly fewer job demands, but are equally prone to developing burnout as their full-time counterparts. The latter finding raises concerns about the sustainability of burnout prevention that focuses on mere work regimes instead of the root causes of burnout.
Physiological and Biochemical Responses of Solanum lycopersicum L. to Benzo[a]pyrene Contaminated Soils
Authors: Voloshina Marina , Rajput Vishnu D. , Chernikova Natalia , Minkina Tatiana , Vechkanov Evgeniy , Mandzhieva Saglara , Voloshin Mark , Krepakova Maria , Dudnikova Tamara , Sushkova Svetlana , Plotnikov Andrey
Published: February 2023

Benzo[a]pyrene (BaP) is noted as one of the main cancer-causing pollutants in human beings and may damage the development of crop plants. The present work was designed to explore more insights into the toxic effects of BaP on Solanum lycopersicum L. at various doses (20, 40, and 60 MPC) spiked in Haplic Chernozem. A dose-dependent response in phytotoxicity were noted, especially in the biomass of the roots and shoots, at doses of 40 and 60 MPC BaP and the accumulation of BaP in S. lycopersicum tissues. Physiological and biochemical response indices were severely damaged based on applied doses of BaP. During the histochemical analysis of the localization of superoxide in the leaves of S. lycopersicum, formazan spots were detected in the area near the leaf’s veins. The results of a significant increase in malondialdehyde (MDA) from 2.7 to 5.1 times, proline 1.12- to 2.62-folds, however, a decrease in catalase (CAT) activity was recorded by 1.8 to 1.1 times. The activity of superoxide dismutase (SOD) increased from 1.4 to 2, peroxidase (PRX) from 2.3 to 5.25, ascorbate peroxidase (APOX) by 5.8 to 11.5, glutathione peroxidase (GP) from 3.8 to 7 times, respectively. The structure of the tissues of the roots and leaves of S. lycopersicum in the variants with BaP changed depending on the dose: it increased the intercellular space, cortical layer, and the epidermis, and the structure of the leaf tissues became looser.
The Use of Collagen-Based Materials in Bone Tissue Engineering
Authors: Fan Lu , Ren Yanru , Emmert Steffen , Vučković Ivica , Stojanovic Sanja , Najman Stevo , Schnettler Reinhard , Barbeck Mike , Schenke-Layland Katja , Xiong Xin
Published: February 2023

Synthetic bone substitute materials (BSMs) are becoming the general trend, replacing autologous grafting for bone tissue engineering (BTE) in orthopedic research and clinical practice. As the main component of bone matrix, collagen type I has played a critical role in the construction of ideal synthetic BSMs for decades. Significant strides have been made in the field of collagen research, including the exploration of various collagen types, structures, and sources, the optimization of preparation techniques, modification technologies, and the manufacture of various collagen-based materials. However, the poor mechanical properties, fast degradation, and lack of osteoconductive activity of collagen-based materials caused inefficient bone replacement and limited their translation into clinical reality. In the area of BTE, so far, attempts have focused on the preparation of collagen-based biomimetic BSMs, along with other inorganic materials and bioactive substances. By reviewing the approved products on the market, this manuscript updates the latest applications of collagen-based materials in bone regeneration and highlights the potential for further development in the field of BTE over the next ten years.
Bio-Guided Isolation of Compounds from Fraxinus excelsior Leaves with Anti-Inflammatory Activity
Authors: Kołtun-Jasion Małgorzata , Sawulska Paulina , Patyra Andrzej , Woźniak Marta , Dudek Marta Katarzyna , Filipek Agnieszka , Kiss Anna Karolina
Published: February 2023

Inflammation is the first physiological defence mechanism against external and internal stimuli. The prolonged or inappropriate response of the immune system may lead to the persistent inflammatory response that can potentially become a basis for chronic diseases e.g., asthma, type II diabetes or cancer. An important role in the alleviation of inflammatory processes, as an adjunct to traditional pharmacological therapy, is attributed to phytotherapy, especially to raw materials with a long tradition of use, e.g., ash leaves. Despite their long-term use in phytotherapy, the specific mechanisms of action have not been confirmed in a sufficient number of biological or clinical studies. The aim of the study is a detailed phytochemical analysis of infusion and its fractions, isolation of pure compounds from the leaves of Fraxinus excelsior and evaluation of their effect on the secretion of anti-inflammatory cytokines (TNF-α, IL-6) and IL-10 receptor expression in an in vitro model of monocyte/macrophage cells isolated from peripheral blood. Methods: Phytochemical analysis was carried out by the UHPLC-DAD-ESI-MS/MS method. Monocytes/macrophages were isolated from human peripheral blood using density gradient centrifugation on Pancoll. After 24 h incubation with tested fractions/subfractions and pure compounds, cells or their supernatants were studied, respectively, on IL-10 receptor expression by flow cytometry and IL-6, TNF-α, IL-1β secretion by the ELISA test. Results were presented with respect to Lipopolysaccharide (LPS) control and positive control with dexamethasone. Results: The infusion, 20% and 50% methanolic fractions and their subfractions, as well as their dominating compounds, e.g., ligstroside, formoside and oleoacteoside isolated from the leaves, show the ability to increase the IL-10 receptor expression on the surface of monocyte/macrophage cells, stimulated by LPS, and to decrease the secretion of pro-inflammatory cytokines, e.g., TNF-α, IL-6.
Lacticaseibacillus rhamnosus dfa1 Attenuate Cecal Ligation-Induced Systemic Inflammation through the Interference in Gut Dysbiosis, Leaky Gut, and Enterocytic Cell Energy
Authors: Tongthong Tongthong , Kaewduangduen Warerat , Phuengmaung Pornpimol , Chancharoenthana Wiwat , Leelahavanichkul Asada
Published: February 2023

Despite an uncommon condition, the clinical management of phlegmon appendicitis (retention of the intra-abdominal appendiceal abscess) is still controversial, and probiotics might be partly helpful. Then, the retained ligated cecal appendage (without gut obstruction) with or without oral Lacticaseibacillus rhamnosus dfa1 (started at 4 days prior to the surgery) was used as a representative model. At 5 days post-surgery, the cecal-ligated mice demonstrated weight loss, soft stool, gut barrier defect (leaky gut using FITC-dextran assay), fecal dysbiosis (increased Proteobacteria with reduced bacterial diversity), bacteremia, elevated serum cytokines, and spleen apoptosis without kidney and liver damage. Interestingly, the probiotics attenuated disease severity as indicated by stool consistency index, FITC-dextran assay, serum cytokines, spleen apoptosis, fecal microbiota analysis (reduced Proteobacteria), and mortality. Additionally, impacts of anti-inflammatory substances from culture media of the probiotics were demonstrated by attenuation of starvation injury in the Caco-2 enterocyte cell line as indicated by transepithelial electrical resistance (TEER), inflammatory markers (supernatant IL-8 with gene expression of TLR4 and NF-κB), cell energy status (extracellular flux analysis), and the reactive oxygen species (malondialdehyde). In conclusion, gut dysbiosis and leaky-gut-induced systemic inflammation might be helpful clinical parameters for patients with phlegmon appendicitis. Additionally, the leaky gut might be attenuated by some beneficial molecules from probiotics.
Gene–Nutrient Interactions in Obesity: COBLL1 Genetic Variants Interact with Dietary Fat Intake to Modulate the Incidence of Obesity
Authors: Kwak Junkyung , Shin Dayeon
Published: February 2023

The COBLL1 gene is associated with leptin, a hormone important for appetite and weight maintenance. Dietary fat is a significant factor in obesity. This study aimed to determine the association between COBLL1 gene, dietary fat, and incidence of obesity. Data from the Korean Genome and Epidemiology Study were used, and 3055 Korean adults aged ≥ 40 years were included. Obesity was defined as a body mass index ≥ 25 kg/m2. Patients with obesity at baseline were excluded. The effects of the COBLL1 rs6717858 genotypes and dietary fat on incidence of obesity were evaluated using multivariable Cox proportional hazard models. During an average follow-up period of 9.2 years, 627 obesity cases were documented. In men, the hazard ratio (HR) for obesity was higher in CT, CC carriers (minor allele carriers) in the highest tertile of dietary fat intake than for men with TT carriers in the lowest tertile of dietary fat intake (Model 1: HR: 1.66, 95% confidence interval [CI]: 1.07–2.58; Model 2: HR: 1.63, 95% CI: 1.04–2.56). In women, the HR for obesity was higher in TT carriers in the highest tertile of dietary fat intake than for women with TT carriers in the lowest tertile of dietary fat intake (Model 1: HR: 1.49, 95% CI: 1.08–2.06; Model 2: HR: 1.53, 95% CI: 1.10–2.13). COBLL1 genetic variants and dietary fat intake had different sex-dependent effects in obesity. These results imply that a low-fat diet may protect against the effects of COBLL1 genetic variants on future obesity risk.
Therapeutic Potential of Linearol in Combination with Radiotherapy for the Treatment of Glioblastoma In Vitro
Authors: Zoi Vasiliki , Papagrigoriou Theodora , Tsiftsoglou Olga S. , Alexiou George A. , Giannakopoulou Maria , Tzima Eftychia , Tsekeris Pericles , Zikou Anastasia , Kyritsis Athanasios P. , Lazari Diamanto , Galani Vasiliki
Published: February 2023

Glioblastoma is one of the most malignant and lethal forms of primary brain tumors in adults. Linearol, a kaurane diterpene isolated from different medicinal plants, including those of the genus Sideritis, has been found to possess significant anti-oxidant, anti-inflammatory and anti-microbial properties. In this study, we aimed to determine whether linearol could exhibit anti-glioma effects when given alone or in combination with radiotherapy in two human glioma cell lines, U87 and T98. Cell viability was examined with the Trypan Blue Exclusion assay, cell cycle distribution was tested with flow cytometry, and the synergistic effects of the combination treatment were analyzed with CompuSyn software. Linearol significantly suppressed cell proliferation and blocked cell cycle at the S phase. Furthermore, pretreatment of T98 cells with increasing linearol concentrations before exposure to 2 Gy irradiation decreased cell viability to a higher extent than linearol or radiation treatment alone, whereas in the U87 cells, an antagonistic relationship was observed between radiation and linearol. Moreover, linearol inhibited cell migration in both tested cell lines. Our results demonstrate for the first time that linearol is a promising anti-glioma agent and further studies are needed to fully understand the underlying mechanism of this effect.
The Role of the Heat Shock Cognate Protein 70 Genes in Sex Determination and Differentiation of Chinese Tongue Sole (Cynoglossus semilaevis)
Authors: Liu Qian , Wang Yue , Tan Leilei , Ma Wenxiu , Zhao Xiaona , Shao Changwei , Wang Qian
Published: February 2023

Fish sex determination can be affected by environmental temperature. This process relies on temperature-sensitive proteins such as heat shock proteins (HSPs). Our previous work found that heat shock cognate proteins (HSCs) may participate in high-temperature associated sex reversal of Chinese tongue sole (Cynoglossus semilaevis). However, the role of hsc genes in responding to high temperature and affecting sex determination/differentiation remains unclear. Here, by using C. semilaevis as model, we identified hsc70 and hsc70-like. hsc70 was abundant in the gonads with a testicular-higher expression at all gonadal development stages except for 6 months post fertilization (mpf). Intriguingly, hsc70-like showed higher expression in testes from 6 mpf on. Both long-term heat treatment during the temperature-sensitive sex-determining period and short-term heat stress at the end of this period caused different expression of hsc70/hsc70-like between sexes. The dual-luciferase assay results also suggested that these genes can respond to high temperature rapidly in vitro. Heat treatment of C. semilaevis testis cells overexpressed with hsc70/hsc70-like could affect the expression of sex-related genes sox9a and cyp19a1a. Our results indicated that hsc70 and hsc70-like were key regulators linking external high-temperature signals with sex differentiation in vivo and provide a new idea for understanding the mechanism by which high temperature affects sex determination/differentiation in teleosts.
Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats
Authors: Bosco Francesca , Guarnieri Lorenza , Nucera Saverio , Scicchitano Miriam , Ruga Stefano , Cardamone Antonio , Maurotti Samantha , Russo Cristina , Coppoletta Anna Rita , Macrì Roberta , Bava Irene , Scarano Federica , Castagna Fabio , Serra Maria , Caminiti Rosamaria , Maiuolo Jessica , Oppedisano Francesca , Ilari Sara , Lauro Filomena , Giancotti Luigi , Muscoli Carolina , Carresi Cristina , Palma Ernesto , Gliozzi Micaela , Musolino Vincenzo , Mollace Vincenzo
Published: February 2023

Skeletal muscle atrophy is a condition characterized by a loss of muscle mass and muscle strength caused by an imbalance between protein synthesis and protein degradation. Muscle atrophy is often associated with a loss of bone mass manifesting as osteoporosis. The aim of this study was to evaluate if chronic constriction injury (CCI) of the sciatic nerve in rats can be a valid model to study muscle atrophy and consequent osteoporosis. Body weight and body composition were assessed weekly. Magnetic resonance imaging (MRI) was performed on day zero before ligation and day 28 before sacrifice. Catabolic markers were assessed via Western blot and Quantitative Real-time PCR. After the sacrifice, a morphological analysis of the gastrocnemius muscle and Micro-Computed Tomography (Micro-CT) on the tibia bone were performed. Rats that underwent CCI had a lower body weight increase on day 28 compared to the naive group of rats (p < 0.001). Increases in lean body mass and fat mass were also significantly lower in the CCI group (p < 0.001). The weight of skeletal muscles was found to be significantly lower in the ipsilateral hindlimb compared to that of contralateral muscles; furthermore, the cross-sectional area of muscle fibers decreased significantly in the ipsilateral gastrocnemius. The CCI of the sciatic nerve induced a statistically significant increase in autophagic and UPS (Ubiquitin Proteasome System) markers and a statistically significant increase in Pax-7 (Paired Box-7) expression. Micro-CT showed a statistically significant decrease in the bone parameters of the ipsilateral tibial bone. Chronic nerve constriction appeared to be a valid model for inducing the condition of muscle atrophy, also causing changes in bone microstructure and leading to osteoporosis. Therefore, sciatic nerve constriction could be a valid approach to study muscle–bone crosstalk and to identify new strategies to prevent osteosarcopenia.
Prolonged Dystocic Labor in Neuraxial Analgesia and the Role of Enkephalin Neurotransmitters: An Experimental Study
Authors: Malvasi Antonio , Cicinelli Ettore , Baldini Giorgio Maria , Vimercati Antonella , Beck Renata , Dellino Miriam , Damiani Gianluca Raffaello , Cazzato Gerardo , Cascardi Eliano , Tinelli Andrea
Published: February 2023

The investigation studied the enkephalinergic neuro fibers (En) contained in the Lower Uterine Segment (LUS) during the prolonged dystocic labor (PDL) with Labor Neuraxial Analgesia (LNA). PDL is generally caused by fetal head malpositions in the Occiput Posterior Position (OPP), Persistent Occiput Posterior Position (POPP), in a transverse position (OTP), and asynclitism (A), and it is detected by Intrapartum Ultrasonography (IU). The En were detected in the LUS samples picked up during cesarean section (CS) of 38 patients undergoing urgent CS in PDL, compared to 37 patients submitted to elective CS. Results were statistically evaluated to understand the differences in En morphological analysis by scanning electron microscopy (SEM) and by fluorescence microscopy (FM). The LUS samples analysis showed an important reduction in En in LUS of CS for the PDL group, in comparison with the elective CS group. The LUS overdistension, by fetal head malpositions (OPP, OTP, A) and malrotations, lead to dystocia, modification of vascularization, and En reduction. The En reduction in PDL suggests that drugs used during the LNA, usually local anesthetics and opioids, cannot control the “dystocic pain”, that differs from normal labor pain. The IU administration in labor and the consequent diagnosis of dystocia suggest stopping the numerous and ineffective top-up drug administration during LNA, and to shift the labor to operative vaginal delivery or CS.
Oxidative Stress and Inflammation in Osteoporosis: Molecular Mechanisms Involved and the Relationship with microRNAs
Authors: Iantomasi Teresa , Romagnoli Cecilia , Palmini Gaia , Donati Simone , Falsetti Irene , Miglietta Francesca , Aurilia Cinzia , Marini Francesca , Giusti Francesca , Brandi Maria Luisa
Published: February 2023

Osteoporosis is characterized by the alteration of bone homeostasis due to an imbalance between osteoclastic bone resorption and osteoblastic bone formation. Estrogen deficiency causes bone loss and postmenopausal osteoporosis, the pathogenesis of which also involves oxidative stress, inflammatory processes, and the dysregulation of the expression of microRNAs (miRNAs) that control gene expression at post-transcriptional levels. Oxidative stress, due to an increase in reactive oxygen species (ROS), proinflammatory mediators and altered levels of miRNAs enhance osteoclastogenesis and reduce osteoblastogenesis through mechanisms involving the activation of MAPK and transcription factors. The present review summarizes the principal molecular mechanisms involved in the role of ROS and proinflammatory cytokines on osteoporosis. Moreover, it highlights the interplay among altered miRNA levels, oxidative stress, and an inflammatory state. In fact, ROS, by activating the transcriptional factors, can affect miRNA expression, and miRNAs can regulate ROS production and inflammatory processes. Therefore, the present review should help in identifying targets for the development of new therapeutic approaches to osteoporotic treatment and improve the quality of life of patients.