bluuebunny/crossref_metadata_2025 huggingface dataset:
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes
Authors: Waletzko Janine , Dau Michael , Seyfarth Anika , Springer Armin , Frank Marcus , Bader Rainer , Jonitz-Heincke Anika
Published: May 2020

Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies.
Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/Polyps with Dysplasia
Authors: Fujiwara-Tani Rina , Fujii Kiyomu , Mori Shiori , Kishi Shingo , Sasaki Takamitsu , Ohmori Hitoshi , Nakashima Chie , Kawahara Isao , Nishiguchi Yukiko , Mori Takuya , Sho Masayuki , Kondoh Masuo , Luo Yi , Kuniyasu Hiroki
Published: May 2020

Sessile serrated adenoma/polyp with dysplasia (SSA/P-D) is an SSA/P with cellular dysplasia and has a higher risk of progressing to colon carcinogenesis. Previously, we reported that tight junction impairment by Clostridium perfringens enterotoxin (CPE) leads to activation of the transcriptional co-activator yes-associated protein (YAP) in oral squamous cell carcinoma. Here, we investigated whether CPE activates YAP to promote the malignant progression of SSA/P. E-cadherin expression was lower in the 12 cases with SSA/P-D examined than that in normal mucosa, SSA/P, or tubular adenoma (TA). Furthermore, intracellular translocation of claudin-4 (CLDN4) and nuclear translocation of YAP were observed. The CPE gene was detected in DNA extracted from SSA/P-D lesions, but not in SSA/P or TA. Treatment of the rat intestinal epithelial cell line IEC6 with low-dose CPE resulted in intracellular translocation of CLDN4 to the cytoplasmic membrane. Cytoplasmic CLDN4 showed co-precipitation with transcriptional co-activator with PDZ-binding motif, zonula occludens (ZO)-1, large tumor suppressor, and mammalian Ste20-like. Additionally, YAP co-precipitated with ZO-2 under CPE treatment led to decreased YAP phosphorylation and nuclear translocation. YAP activation promoted increase in nuclear TEA domain family member level, expression of cyclin D1, snail, vimentin, CD44, NS and decrease in E-cadherin levels, thereby inducing stemness and epithelial-mesenchymal-transition (EMT). The Hippo complex with the incorporation of CLDN4 increased stability. Upon low-dose CPE treatment, HT29 cells with BRAFV600E gene mutation showed increased growth, enhanced invasive potential, stemness, and induced EMT phenotype, whereas HCT116 cells, which carry KRASG13D gene mutation, did not show such changes. In an examination of 10 colorectal cancers, an increase in EMT and stemness was observed in CPE (+) and BRAF mutation (+) cases. These findings suggest that C. perfringens might enhance the malignant transformation of SSA/P-D via YAP activation. Our findings further highlight the importance of controlling intestinal flora using probiotics or antibiotics.
Valosin-Containing Protein, a Calcium-Associated ATPase Protein, in Endoplasmic Reticulum and Mitochondrial Function and Its Implications for Diseases
Authors: Sun Xiaonan , Qiu Hongyu
Published: May 2020

Endoplasmic reticulum (ER) and mitochondrion are the key organelles in mammal cells and play crucial roles in a variety of biological functions in both physiological and pathological conditions. Valosin-containing protein (VCP), a newly identified calcium-associated ATPase protein, has been found to be involved in both ER and mitochondrial function. Impairment of VCP, caused by structural mutations or alterations of expressions, contributes to the development of various diseases, through an integrating effect on ER, mitochondria and the ubiquitin–proteasome system, by interfering with protein degradation, subcellular translocation and calcium homeostasis. Thus, understanding the role and the molecular mechanisms of VCP in these organelles brings new insights to the pathogenesis of the associated diseases, and leads to the discovery of new therapeutic strategies. In this review, we summarized the progress of studies on VCP, in terms of its regulation of ER and mitochondrial function and its implications for the associated diseases, focusing on the cancers, heart disease, and neurodegenerative disorders.
Susceptibility of the Formate Hydrogenlyase Reaction to the Protonophore CCCP Depends on the Total Hydrogenase Composition
Authors: Telleria Marloth Janik , Pinske Constanze
Published: May 2020

Fermentative hydrogen production by enterobacteria derives from the activity of the formate hydrogenlyase (FHL) complex, which couples formate oxidation to H2 production. The molybdenum-containing formate dehydrogenase and type-4 [NiFe]-hydrogenase together with three iron-sulfur proteins form the soluble domain, which is attached to the membrane by two integral membrane subunits. The FHL complex is phylogenetically related to respiratory complex I, and it is suspected that it has a role in energy conservation similar to the proton-pumping activity of complex I. We monitored the H2-producing activity of FHL in the presence of different concentrations of the protonophore CCCP. We found an inhibition with an apparent EC50 of 31 µM CCCP in the presence of glucose, a higher tolerance towards CCCP when only the oxidizing hydrogenase Hyd-1 was present, but a higher sensitivity when only Hyd-2 was present. The presence of 200 mM monovalent cations reduced the FHL activity by more than 20%. The Na+/H+ antiporter inhibitor 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) combined with CCCP completely inhibited H2 production. These results indicate a coupling not only between Na+ transport activity and H2 production activity, but also between the FHL reaction, proton import and cation export.
Effect of One- and Two-Stage Shear Cutting on the Fatigue Strength of Truck Frame Parts
Authors: Stahl Jens , Pätzold Isabella , Golle Roland , Sunderkötter Christina , Sieurin Henrik , Volk Wolfram
Published: May 2020

The longitudinal and transverse beams of trucks are manufactured with a large number of holes to fasten brackets for springs, fuel tanks, batteries etc. The quality of the holes, which is particularly influenced by the manufacturing process, has a major influence on the fatigue strength of the beams and thus the service lifetime of the vehicle. In most cases, the holes are produced using the highly economical shear cutting process. Previous investigations have shown that the fatigue strength of thin sheets can be increased by adjusting the shearing parameters and using a two-stage shear cutting process. This paper discusses the difference between one- and two-stage shear cutting on the hole quality of components made of S500MC (1.0984, thickness 8 mm) and its resulting fatigue strength. The hole quality is characterized by the geometry of the shear cut surface, its roughness, microstructure, and microhardness. It was shown that the two-stage shear cutting process allows producing holes of better quality than the ones manufactured by a one-stage shear cutting process. Furthermore, this resulted in an improved fatigue behavior.
Methodology for Carrying out Measurements of the Tombolo Geomorphic Landform Using Unmanned Aerial and Surface Vehicles near Sopot Pier, Poland
Authors: Specht Cezary , Lewicka Oktawia , Specht Mariusz , Dąbrowski Paweł , Burdziakowski Paweł
Published: May 2020

The human impact on the ecosystem has been particularly evident in the last century; it transforms the Earth’s surface on an unprecedented scale and brings about irreversible changes. One example is an oceanographic phenomenon known as a tombolo, i.e., a narrow belt connecting the mainland with an island lying near the shore formed as a result of sand and gravel being deposited by sea currents. The phenomenon contributes to an increase in the biogenic substance content in the littoral zone, which leads to increased cyanobacteria blooming in the summer period. Moreover, the debris accumulation in the littoral zone results in the mud formation, which makes the beach landscape less attractive. One of the main features of the tombolo phenomenon is its variability of shape, which includes the form of both the shore and the seabed adjacent to it. Therefore, to describe its size and spatio-temporal variability, it is necessary to apply methods for geodetic (the land) and hydrographic (the sea) measurements that can be carried out in different ways. The aim of the paper is to present the methodology for carrying out measurements of the tombolo oceanographic phenomenon using Unmanned Aerial Vehicles (UAV) and Unmanned Surface Vehicles (USV) on the example of a waterbody adjacent to the Sopot pier. It also presents the results of surveys carried out in November 2019 within this area. The study demonstrated that the integration of two measuring devices whose development began in the second decade of the 20th century, i.e., UAVs and USVs, enables accurate (even up to several centimeters) and reliable determination of the scale and variability of the phenomena occurring in the littoral zone.
On the Need for an Integrated Large-Scale Methodology of Coastal Management: A Methodological Proposal
Authors: Armenio Elvira , Mossa Michele
Published: May 2020

Sustainable management of coastal areas involves dealing with problems such as coastal erosion, rapid growth in the rate of urbanization, tourism, environmental degradation associated with industrial and urban activities. Besides consideration is provided for the effects of climate change, whose scenarios also have significant consequences on coastal systems that are already extremely vulnerable and prone to many human pressures. Over the years, several international and national studies have been conducted to deepen the coastal processes. To date—despite considerable efforts—there are still problems. Two relevant priorities emerge: managing coastal risks and ensuring sustainable coastal management. In response to the above-mentioned challenges, it is worthwhile to elaborate an integrated methodology that, basing on the collection, analysis and evaluation of data, may provide an effective guideline for the successful implementation of each action, while providing timely and targeted information for the adoption of governance strategies concerning the prevention and management of marine-coastal risks. In the present study, considering what emerged in the major research projects on the coastal field during the last decades, a methodological proposal is outlined to pursue the principles of the integrated coastal zona management (ICZM) and join the managing coastal risks with sustainable uses focusing on the implementation scale.
How Effective Were the Beach Nourishments at Cancun?
Authors: Martell Raúl , Mendoza Edgar , Mariño-Tapia Ismael , Odériz Itxaso , Silva Rodolfo
Published: May 2020

Beach nourishment is generally seen as the preferred means of rectifying coastal erosion, due to its low environmental impact and natural evolution. The largest beach nourishment project ever carried out in Mexico took place on Cancun beach in 2006, as a response to the most intense hurricane season ever registered in Mexico, in 2005. After Hurricane Dean, in 2009, a second nourishment was conducted, which evidenced flaws in the design and execution of the first project. Previous investigations report that the need for beach re-fills directly correlates with wave energy. However, following a thorough revision of the extreme climatic events that occurred between 1978 and 2018, it has been found that the amount of erosion also depends on the frequency and duration of high energy events. The findings also show that the apparent success of the second nourishment is mainly associated with a decline in the number of extreme wave power events impacting the beach. In the conclusion to this paper, we share the knowledge gained, but not yet applied, in Mexico or elsewhere, regarding beach use, urbanization, and protection in beach planning.
Two-Dimensional Numerical Modelling of a Moored Floating Body under Sloping Seabed Conditions
Authors: Feng Aichun , Kang Hooi Siang , Zhao Binbin , Jiang Zhiyu
Published: May 2020

A coupled floating body-mooring line model is developed by combining a boundary element model for a two-dimensional floating body and a catenary mooring line model. The boundary element model is formulated in the time domain by a continuous Rankine source, and a reflection potential is introduced to account for the wave reflection due to sloping seabed. This newly developed model is validated by comparisons against available data. Then, dynamic response analyses are performed for the moored body in various seabed conditions. Compared with a flat seabed, a sloping seabed causes unsymmetrical mooring line configuration and generates noticeable effects in the motion responses of the floating body.
Dynamic Optimal Dispatch of Energy Systems with Intermittent Renewables and Damage Model
Authors: Kim Rebecca , Wang Yifan , Vudata Sai Pushpitha , Bhattacharyya Debangsu , Lima Fernando V. , Turton Richard
Published: May 2020

With the increasing penetration of intermittent renewable energy sources into the grid, there is a growing need for process systems-based strategies that integrate dispatchable and variable energy systems for supplying the demand while maintaining grid reliability. The proposed framework corresponds to a dynamic mixed-integer linear programming optimization approach that integrates coal-fired and natural gas-fired power plants, NaS batteries for energy storage, and solar/wind energy to supply the demand. This optimization approach considers an economic goal and constraints to provide power balance while maintaining the overall damage of the natural gas combined cycle (NGCC) power plant drum under a maximum stress as well as avoiding the overheating of the NGCC superheater and reheater. Renewable curtailment levels are also retained at minimum levels. Case studies are analyzed considering different loads and renewable penetration levels. The results show that the demand was met for all cases. Grid flexibility was mostly provided by the NGCC, while the batteries were used sparingly. In addition, considering a CO2 equivalent analysis, the environmental performance was intrinsically connected to grid flexibility and the level of renewable penetration. Stress analysis results reinforced the necessity for an equipment health-related constraint.
On the Aα-Spectral Radii of Cactus Graphs
Authors: Wang Chunxiang , Wang Shaohui , Liu Jia-Bao , Wei Bing
Published: May 2020

Let A ( G ) be the adjacent matrix and D ( G ) the diagonal matrix of the degrees of a graph G, respectively. For 0 ≤ α ≤ 1 , the A α -matrix is the general adjacency and signless Laplacian spectral matrix having the form of A α ( G ) = α D ( G ) + ( 1 − α ) A ( G ) . Clearly, A 0 ( G ) is the adjacent matrix and 2 A 1 2 is the signless Laplacian matrix. A cactus is a connected graph such that any two of its cycles have at most one common vertex, that is an extension of the tree. The A α -spectral radius of a cactus graph with n vertices and k cycles is explored. The outcomes obtained in this paper can imply some previous bounds from trees to cacti. In addition, the corresponding extremal graphs are determined. Furthermore, we proposed all eigenvalues of such extremal cacti. Our results extended and enriched previous known results.
Modular Uniform Convexity in Every Direction in Lp(·) and Its Applications
Authors: Bachar Mostafa , Méndez Osvaldo
Published: May 2020

We prove that the Lebesgue space of variable exponent L p ( · ) ( Ω ) is modularly uniformly convex in every direction provided the exponent p is finite a.e. and different from 1 a.e. The notion of uniform convexity in every direction was first introduced by Garkavi for the case of a norm. The contribution made in this work lies in the discovery of a modular, uniform-convexity-like structure of L p ( · ) ( Ω ) , which holds even when the behavior of the exponent p ( · ) precludes uniform convexity of the Luxembourg norm. Specifically, we show that the modular ρ ( u ) = ∫ Ω | u ( x ) | d x possesses a uniform-convexity-like structure even if the variable exponent is not bounded away from 1 or ∞. Our result is new and we present an application to fixed point theory.
Erratum: Al-Marzouki, S., et al. Type II Topp Leone Power Lomax Distribution with Applications. Mathematics 2020, 8, 4
Authors: Al-Marzouki Sanaa , Jamal Farrukh , Chesneau Christophe , Elgarhy Mohammed
Published: May 2020

Due to a lapse, there was wrong funding information in the original article version [...]
Reasons for and Facilitating Factors of Medical Malpractice Complaints. What Can Be Done to Prevent Them?
Authors: Hanganu Bianca , Iorga Magdalena , Muraru Iulia-Diana , Ioan Beatrice Gabriela
Published: May 2020

Background and objectives. Medical malpractice is an increasing phenomenon all over the world, and Romania is not spared. This matter is of concern as it has a significant impact on the physicians and the patients involved, as well as on the health care system and society in general. The purpose of our study was to perform an insight analysis on the reasons for medical malpractice complaints as well as the factors that facilitate the complaints to identify specific ways to prevent them and, implicitly, to improve the medical practice. Materials and Methods. The authors conducted a retrospective study of the medical malpractice complaints registered in the period 2006–2019 at the Commission for monitoring and professional competence for malpractice cases in the region of Moldova, Romania, collecting data on both the patients and the medical professionals involved. Results. The authors analyzed 153 complaints directed against 205 medical professionals and identified 15 categories of reasons for complaints, the most significant being related to the occurrence of complications, and to the doctor–patient interaction (e.g., communication, behavior, informed consent). The most frequently reported medical specialties were obstetrics and gynecology, emergency medicine, general surgery, and orthopedics and traumatology. Emergency medicine was often involved in complaints suggesting an over utilization of this department in our country and the need for health policies, which could divert the large number of patients accessing emergency medicine towards primary care. Conclusions. Regarding the dysfunctions in the doctor–patient relationship frequently claimed by patients, the authors concluded that doctors need special undergraduate training and periodic updating during their practice for them to be able to adequately address the challenges of interacting with their patients.
Effects of Cesarean Section and Vaginal Delivery on Abdominal Muscles and Fasciae
Authors: Fan Chenglei , Guidolin Diego , Ragazzo Serena , Fede Caterina , Pirri Carmelo , Gaudreault Nathaly , Porzionato Andrea , Macchi Veronica , De Caro Raffaele , Stecco Carla
Published: May 2020

Background and objectives: Possible disorders after delivery may interfere with the quality of life. The aim of this study was to ascertain whether abdominal muscles and fasciae differ in women depending on whether they experienced transverse cesarean section (CS) or vaginal delivery (VA) in comparison with healthy nulliparous (NU). Materials and methods: The thicknesses of abdominal muscles and fasciae were evaluated by ultrasound in 13 CS, 10 VA, and 13 NU women (we examined rectus abdominis (RA); external oblique (EO); internal oblique (IO); transversus abdominis (TrA); total abdominal muscles (TAM = EO + IO + TrA); inter-rectus distance (IRD); thickness of linea alba (TLA); rectus sheath (RS), which includes anterior fascia of RS and posterior fascia of RS (P-RS); loose connective tissue between sublayers of P-RS (LCT); abdominal perimuscular fasciae (APF), which includes anterior fascia of EO, fasciae between EO, IO, and TrA, and posterior fascia of TrA). Data on pain intensity, duration, and location were collected. Results: Compared with NU women, CS women had wider IRD (p = 0.004), thinner left RA (p = 0.020), thicker right RS (p = 0.035) and APF (left: p = 0.001; right: p = 0.001), and IO dissymmetry (p = 0.009). VA women had thinner RA (left: p = 0.008, right: p = 0.043) and left TAM (p = 0.024), mainly due to left IO (p = 0.027) and RA dissymmetry (p = 0.035). However, CS women had thicker LCT (left: p = 0.036, right: p < 0.001), APF (left: p = 0.014; right: p = 0.007), and right IO (p = 0.028) than VA women. There were significant correlations between pain duration and the affected fasciae/muscles in CS women. Conclusions: CS women showed significant alterations in both abdominal fasciae and muscle thicknesses, whereas VA women showed alterations mainly in muscles. Thinner RA and/or dissymmetric IO, wider IRD, and thicker LCT and APF after CS may cause muscle deficits and alteration of fascial gliding, which may induce scar, abdominal, low back, and/or pelvic pain.
Male Anabolic Androgenic Steroid Users with Personality Disorders Report More Aggressive Feelings, Suicidal Thoughts, and Criminality
Authors: Börjesson Annica , Möller Christian , Hagelin Anders , Vicente Veronica , Rane Anders , Lehtihet Mikael , Dahl Marja-Liisa , Gårevik Nina , Ekström Lena
Published: May 2020

Background and objectives: Anabolic androgenic steroids (AAS) are mainly used for aesthetic and performance-enhancing reasons. Their use is a growing public health problem and concern for society because of their adverse effects. The primary aim of this study was to identify psychiatric and personality disorders and to measure anxiety and depression in AAS users. Materials and Methods: Fifty-six males who actively contacted the Anti-Doping Hot-Line and wished to stop using AAS were included. Structured Clinical Interviews Diagnosis-I and -II were used to diagnose psychiatric and personality disorders. The Brief Scale for Anxiety and Montgomery Asberg Depression Rating Scale (subscales from the Comprehensive Psychopathological Rating Scale) were used to measure changes in anxiety and depression. Structured Clinical Interviews Diagnosis-I and -II were performed at one time point. Anxiety and depression were measured at inclusion and after six months. Urine samples were collected for an analysis of AAS and drugs of abuse. Results: All participants reported some adverse effects that they associated with AAS use. In total, 56% and 52% of the cohort fulfilled the criteria for Structured Clinical Interviews Diagnosis-I and -II diagnoses, respectively. A significantly increased risk of reporting aggressive feelings/behaviors (Odds Ratio (OR) = 4.9; Confidence Interval (CI) 0.99–25, p = 0.04), suicidal thoughts/attempts (OR = 4.6, CI 95; 0.99–21, p = 0.04) and criminality (OR = 6.5, CI 1–39, p = 0.03) was found among individuals with AAS use fulfilling the criteria for personality disorders compared with those without such AAS use. The Brief Scale for Anxiety score decreased from the median of 15 at inclusion to 10 at the follow-up visit six months later (p = 0.01, n = 19). Conclusions: Our findings indicate that among individuals with AAS use, those with a personality disorder report more aggressive behaviors, suicidal thoughts/suicidal attempts, and criminality than those without a personality disorder.
Extension of Barlat’s Yield Criterion to Tension–Compression Asymmetry: Modeling and Verification
Authors: Chen Lei , Zhang Hongying , Song Mitao
Published: May 2020

The present study is devoted to extending Barlat’s famous yield criteria to tension–compression asymmetry by a novel method originally introduced by Khan, which can decouple the anisotropy and tension–compression asymmetry characteristics. First, Barlat (1987) isotropic yield criterion, which leads to a good approximation of yield loci calculated by the Taylor–Bishop–Hill crystal plasticity model, is extended to include yielding asymmetry. Furthermore, the famous Barlat (1989) anisotropic yield criterion, which can well describe the plastic behavior of face-centered cubic (FCC) metals, is extended to take the different strength effects into account. The proposed anisotropic yield criterion has a simple mathematical form and has only five parameters when used in planar stress states. Compared with existing theories, the new yield criterion has much fewer parameters, which makes it very convenient for practical applications. Furthermore, all coefficients of the criterion can be determined by explicit expressions. The effectiveness and flexibility of the new yield criterion have been verified by applying to different materials. Results show that the proposed theory can describe the plastic anisotropy and yielding asymmetry of metals well and the transformation onset of the shape memory alloy, showing excellent predictive ability and flexibility.
Crosstalk Between Culturomics and Microbial Profiling of Egyptian Mongoose (Herpestes ichneumon) Gut Microbiome
Authors: Pereira André C. , Bandeira Victor , Fonseca Carlos , Cunha Mónica V.
Published: May 2020

Recently, we unveiled taxonomical and functional differences in Egyptian mongoose (Herpestes ichneumon) gut microbiota across sex and age classes by microbial profiling. In this study, we generate, through culturomics, extended baseline information on the culturable bacterial and fungal microbiome of the species using the same specimens as models. Firstly, this strategy enabled us to explore cultivable microbial community differences across sexes and to ascertain the influence exerted by biological and environmental contexts of each host in its microbiota signature. Secondly, it permitted us to compare the culturomics and microbial profiling approaches and their ability to provide information on mongoose gut microbiota. In agreement with microbial profiling, culturomics showed that the core gut cultivable microbiota of the mongoose is dominated by Firmicutes and, as previously found, is able to distinguish sex- and age class-specific genera. Additional information could be obtained by culturomics, with six new genera unveiled. Richness indices and the Shannon index were concordant between culture-dependent and culture-independent approaches, highlighting significantly higher values when using microbial profiling. However, the Simpson index underlined higher values for the culturomics-generated data. These contrasting results were due to a differential influence of dominant and rare taxa on those indices. Beta diversity analyses of culturable microbiota showed similarities between adults and juveniles, but not in the data series originated from microbial profiling. Additionally, whereas the microbial profiling indicated that there were several bioenvironmental features related to the bacterial gut microbiota of the Egyptian mongoose, a clear association between microbiota and bioenvironmental features could not be established through culturomics. The discrepancies found between the data generated by the two methodologies and the underlying inferences, both in terms of β-diversity and role of bioenvironmental features, confirm that culture-independent, sequence-based methods have a higher ability to assess, at a fine scale, the influence of abiotic and biotic factors on the microbial community composition of mongoose’ gut. However, when used in a complementary perspective, this knowledge can be expanded by culturomics.
Natural Function and Structural Modification of Climacostol, a Ciliate Secondary Metabolite
Authors: Buonanno Federico , Catalani Elisabetta , Cervia Davide , Cimarelli Cristina , Marcantoni Enrico , Ortenzi Claudio
Published: May 2020

The review highlights the main results of two decades of research on climacostol (5-[(2Z)-non-2-en-1-yl]benzene-1,3-diol), the resorcinolic lipid produced and used by the ciliated protozoan Climacostomum virens for chemical defense against a wide range of predators, and to assist its carnivorous feeding. After the first studies on the physiological function of climacostol, the compound and some analogues were chemically synthesized, thus allowing us to explore both its effect on different prokaryotic and eukaryotic biological systems, and the role of its relevant structural traits. In particular, the results obtained in the last 10 years indicate climacostol is an effective antimicrobial and anticancer agent, bringing new clues to the attempt to design and synthesize additional novel analogues that can increase or optimize its pharmacological properties.
Long-Term Antibiotic Prophylaxis in Urology and High Incidence of Clostridioides difficile Infections in Surgical Adult Patients
Authors: Jachowicz Estera , Wałaszek Marta , Sulimka Grzegorz , Maciejczak Andrzej , Zieńczuk Witold , Kołodziej Damian , Karaś Jacek , Pobiega Monika , Wójkowska-Mach Jadwiga
Published: May 2020

Clostridioides difficile infections are the main cause of antibiotic-related diarrhea. Most of them come in the form of healthcare-associated Clostridioides difficile infections (HA-CDI). The aim of the study was to analyze HA-CDI epidemiology and the relationship between antibiotic consumption and CDI epidemiology at St Luke’s Provincial Hospital in Tarnow, Poland. In 2012–2018, surveillance of CDI was carried out in adult surgical wards at St Luke’s Provincial Hospital. The data were collected in accordance with the methodology of the Healthcare-Associated Infections Surveillance Network (HAI-Net), European Centre for Disease Prevention and Control (ECDC), and the ATC/DDD system (Anatomical Therapeutic Chemical Classification System) of the World Health Organization. In total, in the study period, 51 cases of CDI involved CA-CDI (24.5%) and 147 were HA-CDIs (75.5%). The most CA-CDIs were found in the general surgery (32.6%) and urology (17.0%) wards. CA-CDI incidence was 0.7/1000 patients and for HA-CDI it was 2/1000 patients (4.4/10,000 patientdays (pds)). The highest HA-CDI incidence was in the neurosurgical departments (18/10,000 pds) and oncological surgery (8.4/10,000) pds. There was a significant positive correlation between CA-CDI and HA-CDI (correlation of 0.943, p < 0.001) and between the number of patients hospitalized and HA-CDI (correlation of 0.865, p = 0.012). The total antibiotic consumption amounted to 0.7 DDD/10,000 pds; it was the highest in the urology ward (0.84/10,000 pds) and 49.5% of the antibiotics were fluoroquinolones (0.41/10,000 pds). On the basis of regression coefficients, a positive correlation was demonstrated between the use of fluoroquinolones and the HA-CDI incidence rate. Both a high percentage of CDI cases and a high intake of antibiotics were recorded in the urology department. About half of all antibiotics were fluoroquinolones.
Characterization of Indigenous Lactic Acid Bacteria in Cow Milk of the Maltese Islands: A Geographical and Seasonal Assessment
Authors: Garroni Elisa , Doulgeraki Agapi I. , Pavli Foteini , Spiteri David , Valdramidis Vasilis P.
Published: May 2020

A geographical and seasonal assessment of indigenous lactic acid bacteria (LAB) in Maltese cow milk was conducted in this study. To investigate this, milk was collected from different regions of Malta during winter and summer seasons. Total viable counts (TVC) and LAB population were enumerated. Afterwards, LAB were isolated and identified by molecular methods. According to the results, similar TVC were enumerated on winter and summer samples, while highest LAB population was detected on summer samples. LAB isolates were grouped in seven different clusters which were assigned to Lactobacillus casei, Pediococcus pentosaceus, Lactobacillus plantarum, Weissella paramesenteroides, Lactobacillus rhamnosus, Lactococcus lactis, and Lactococcus garvieae. In addition, Enterococcus and Streptococcus species were also isolated. Season seemed to affect the genus/species of LAB since Lactobacillus were mainly isolated from winter samples, while Lactococcus and Enterococcus species were the main genera identified in summer samples. Regarding the geographical distribution, the majority of the Lactobacillus spp. were isolated from the South-eastern region in both seasons. In conclusion, through this study the diversity of indigenous LAB in the Maltese cow milk was monitored for the first time and highlighted that the microbial communities are affected by seasonality and geographical distribution of the farms.
The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
Authors: Stojković Ljiljana , Jovanović Ivan , Zivković Maja , Zec Manja , Djurić Tamara , Zivotić Ivan , Kuveljić Jovana , Kolaković Ana , Kolić Ivana , Djordjević Ana , Glibetić Marija , Alavantić Dragan , Stanković Aleksandra
Published: May 2020

Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
Glycemic Index (GI) or Glycemic Load (GL) and Dietary Interventions for Optimizing Postprandial Hyperglycemia in Patients with T2 Diabetes: A Review
Authors: Vlachos Dionysios , Malisova Sofia , Lindberg Fedon A. , Karaniki Georgia
Published: May 2020

The increasing prevalence of type 2 diabetes (T2D) worldwide calls for effective approaches to its management. Strategies for diabetes have generally focused on optimizing overall glycemic control as assessed by glycated hemoglobin (HbA1c) and fasting plasma glucose (FPG) values. However, since 2001, the American Diabetes Association has established postprandial glucose (PPG) as an independent contributor to both HbA1c and diabetes complications, and increasing evidence suggests that all three glycemic parameters of HbA1c, FPG, and postprandial glucose (PPG) are independently important. Objectives: The objective of this review was to comprehensively summarize the literature on the effects of nutritional strategies incorporating glycemic index (GI)/glycemic load (GL) on the postprandial hyperglycemia in people with T2D, as well as to provide recommendations for effective dietary strategies addressing both the dietary glycemic index and load in clinical practice. Design: An advanced Pubmed search was conducted. A total of 10 randomized controlled studies met the inclusion criteria. Six studies compared low-GI with higher GI meals, three included studies that compared reduced carbohydrate content with higher carbohydrate content, and one study compared meals of low-GI (with high or low fiber) with meals of higher GI (with high or low fiber). Results: Most of the clinical trials resulted in significant improvement (p < 0.05) of postprandial hyperglycemia. Conclusions: Either reducing the amount of carbohydrate in a meal or increasing consumption of soluble fiber has a favorable effect on postprandial glucose excursions.
Dietary Carbohydrate Intake, Dietary Glycemic Load and Outcomes of In Vitro Fertilization: Findings from an Observational Italian Cohort Study
Authors: Noli Stefania Antonia , Ricci Elena , Cipriani Sonia , Ferrari Stefania , Castiglioni Marta , La Vecchia Irene , Somigliana Edgardo , Parazzini Fabio
Published: May 2020

In recent decades, increasing attention has been paid to the influence of diet on reproductive health. Carbohydrates in diet affect glucose metabolism and multiple evidences showed the key role of insulin sensitivity in regulating female fertility. We designed a prospective cohort study to investigate the relation between dietary carbohydrate intake, glycemic load (GL) and the outcomes of assisted reproduction. A population of 494 female partners of couples referring to an Italian Fertility Center and eligible for in vitro fertilization (IVF) were enrolled in the study. On the day of the oocyte retrieval, information on their diet was obtained using a validated food frequency questionnaire (FFQ). We calculated the relative risk and 95% confidence interval of embryo transfer, clinical pregnancy and live birth according to the following dietary exposures: GL, glycemic index (GI) as well as the daily carbohydrate and fiber intake. A multiple regression model was used to account for the confounders. After adjusting for age, college degree, body mass index (BMI), leisure physical activity and previous assisted reproduction techniques (ART) cycles, no significant association was observed between the considered dietary exposures and the IVF outcomes. The roles of GL, carbohydrate intake and GI were assessed in strata of the cause of infertility and body mass index and no relation emerged in this further analysis. We found no clear association between the dietary carbohydrate quantity and quality and IVF outcomes in a cohort of infertile Italian women.
Effect of Praziquantel on Schistosoma mekongi Proteome and Phosphoproteome
Authors: Chienwichai Peerut , Ampawong Sumate , Adisakwattana Poom , Thiangtrongjit Tipparat , Limpanont Yanin , Chusongsang Phiraphol , Chusongsang Yupa , Reamtong Onrapak
Published: May 2020

Schistosoma mekongi causes schistosomiasis in southeast Asia, against which praziquantel (PZQ) is the only treatment option. PZQ resistance has been reported, thus increasing the requirement to understand mechanism of PZQ. Herein, this study aimed to assess differences in proteome and phosphoproteome of S. mekongi after PZQ treatment for elucidating its action. Furthermore, key kinases related to PZQ effects were predicted to identify alternative targets for novel drug development. Proteomes of S. mekongi were profiled after PZQ treatment at half maximal inhibitory concentration and compared with untreated worms. A total of 144 proteins were differentially expressed after treatment. In parallel, immunohistochemistry indicated a reduction of phosphorylation, with 43 phosphoproteins showing reduced phosphorylation, as identified by phosphoproteomic approach. Pathway analysis of mass spectrometric data showed that calcium homeostasis, worm antigen, and oxidative stress pathways were influenced by PZQ treatment. Interestingly, two novel mechanisms related to protein folding and proteolysis through endoplasmic reticulum-associated degradation pathways were indicated as a parasiticidal mechanism of PZQ. According to kinase–substrate predictions with bioinformatic tools, Src kinase was highlighted as the major kinase related to the alteration of phosphorylation by PZQ. Interfering with these pathways or applying Src kinase inhibitors could be alternative approaches for further antischistosomal drug development.
Strongyloides stercoralis in Alcoholic Patients: Implications of Alcohol Intake in the Frequency of Infection and Parasite Load
Authors: de Souza Joelma N. , Oliveira Cíntia de L. , Araújo Wéslei A. C. , Souza Alex B. S. , Silva Mônica L. S. , da Cruz Irlana D. R. , Sampaio Larissa M. , dos Santos Juliane S. B. , Teixeira Márcia C. A. , Soares Neci M.
Published: May 2020

Strongyloides stercoralis infection in immunocompromised subjects, including chronic alcoholics, can lead to a severe disease. Moreover, its prevalence in alcoholic patients seems to be higher than that in the general population. The aims of this study were to evaluate the frequency of S. stercoralis infection in alcoholic patients and to investigate the influence of alcohol intake on the parasite load, as well as to evaluate the sensitivity of three different parasitological methods according to the larval output. Fecal samples of 1290 chronic alcoholic patients were examined by spontaneous sedimentation, Baermann–Moraes, and agar plate culture (APC) methods. S. stercoralis was the most frequent parasite found (14.5%; n = 187). Alcoholic individuals infected with Strongyloides stercoralis had a higher daily consumption of alcohol than those who were not infected, 528.6 and 403.0 g/day, respectively (p < 0.05). In addition, individuals with higher alcohol intake presented an increase in parasite load. The S. stercoralis diagnostic method with the highest sensitivity was APC, 97.9% (183/187). In conclusion, S. stercoralis seems to be the most frequent parasite found in alcoholic individuals from endemic areas and alcohol intake is positively associated with S. stercoralis larvae output. In addition, this study confirms that APC is the most sensitive parasitological method used for Strongyloides diagnosis.
Quantum Hopfield Model
Authors: Shcherbina Masha , Tirozzi Brunello , Tassi Camillo
Published: May 2020

We find the free-energy in the thermodynamic limit of a one-dimensional XY model associated to a system of N qubits. The coupling among the σ i z is a long range two-body random interaction. The randomness in the couplings is the typical interaction of the Hopfield model with p patterns ( p < N ), where the patterns are p sequences of N independent identically distributed random variables (i.i.d.r.v.), assuming values ± 1 with probability 1 / 2 . We show also that in the case p ≤ α N , α ≠ 0 , the free-energy is asymptotically independent from the choice of the patterns, i.e., it is self-averaging.
Design and FDM/FFF Implementation of a Compact Omnidirectional Wheel for a Mobile Robot and Assessment of ABS and PLA Printing Materials
Authors: Rubies Elena , Palacín Jordi
Published: May 2020

This paper proposes the design and 3D printing of a compact omnidirectional wheel optimized to create a small series of three-wheeled omnidirectional mobile robots. The omnidirectional wheel proposed is based on the use of free-rotating passive wheels aligned transversally to the center of the main wheel and with a constant separation gap. This paper compares a three inner-passive wheels design based on mass-produced parts and 3D printed elements. The inner passive wheel that better combines weight, cost, and friction is implemented with a metallic ball bearing fitted inside a 3D printed U-grooved ring that holds a soft toric joint. The proposed design has been implemented using acrylonitrile butadiene styrene (ABS) and tough polylactic acid (PLA) as 3D printing materials in order to empirically compare the deformation of the weakest parts of the mechanical design. The conclusion is that the most critical parts of the omnidirectional wheel are less prone to deformation and show better mechanical properties if they are printed horizontally (with the axes that hold the passive wheels oriented parallel to the build surface), with an infill density of 100% and using tough PLA rather than ABS as a 3D printing material.
GRID: A Python Package for Field Plot Phenotyping Using Aerial Images
Authors: Chen Chunpeng James , Zhang Zhiwu
Published: May 2020

Aerial imagery has the potential to advance high-throughput phenotyping for agricultural field experiments. This potential is currently limited by the difficulties of identifying pixels of interest (POI) and performing plot segmentation due to the required intensive manual operations. We developed a Python package, GRID (GReenfield Image Decoder), to overcome this limitation. With pixel-wise K-means cluster analysis, users can specify the number of clusters and choose the clusters representing POI. The plot grid patterns are automatically recognized by the POI distribution. The local optima of POI are initialized as the plot centers, which can also be manually modified for deletion, addition, or relocation. The segmentation of POI around the plot centers is initialized by automated, intelligent agents to define plot boundaries. A plot intelligent agent negotiates with neighboring agents based on plot size and POI distributions. The negotiation can be refined by weighting more on either plot size or POI density. All adjustments are operated in a graphical user interface with real-time previews of outcomes so that users can refine segmentation results based on their knowledge of the fields. The final results are saved in text and image files. The text files include plot rows and columns, plot size, and total plot POI. The image files include displays of clusters, POI, and segmented plots. With GRID, users are completely liberated from the labor-intensive task of manually drawing plot lines or polygons. The supervised automation with GRID is expected to enhance the efficiency of agricultural field experiments.