bluuebunny/crossref_metadata_2025 huggingface dataset:
Interpretation of Single-Molecule Force Experiments on Proteins Using Normal Mode Analysis
Authors: Bauer Jacob , Žoldák Gabriel
Published: October 2021

Single-molecule force spectroscopy experiments allow protein folding and unfolding to be explored using mechanical force. Probably the most informative technique for interpreting the results of these experiments at the structural level makes use of steered molecular dynamics (MD) simulations, which can explicitly model the protein under load. Unfortunately, this technique is computationally expensive for many of the most interesting biological molecules. Here, we find that normal mode analysis (NMA), a significantly cheaper technique from a computational perspective, allows at least some of the insights provided by MD simulation to be gathered. We apply this technique to three non-homologous proteins that were previously studied by force spectroscopy: T4 lysozyme (T4L), Hsp70 and the glucocorticoid receptor domain (GCR). The NMA results for T4L and Hsp70 are compared with steered MD simulations conducted previously, and we find that we can recover the main results. For the GCR, which did not undergo MD simulation, our approach identifies substructures that correlate with experimentally identified unfolding intermediates. Overall, we find that NMA can make a valuable addition to the analysis toolkit for the structural analysis of single-molecule force experiments on proteins.
Gas Phase Synthesis of Multi-Element Nanoparticles
Authors: López-Martín Raúl , Burgos Benito Santos , Normile Peter S. , De Toro José A. , Binns Chris
Published: October 2021

The advantages of gas-phase synthesis of nanoparticles in terms of size control and flexibility in choice of materials is well known. There is increasing interest in synthesizing multi-element nanoparticles in order to optimize their performance in specific applications, and here, the flexibility of material choice is a key advantage. Mixtures of almost any solid materials can be manufactured and in the case of core–shell particles, there is independent control over core size and shell thickness. This review presents different methods of producing multi-element nanoparticles, including the use of multiple targets, alloy targets and in-line deposition methods to coat pre-formed cores. It also discusses the factors that produce alloy, core–shell or Janus morphologies and what is possible or not to synthesize. Some applications of multi-element nanoparticles in medicine will be described.
Novel Biocompatible with Animal Cells Composite Material Based on Organosilicon Polymers and Fullerenes with Light-Induced Bacteriostatic Properties
Authors: Gudkov Sergey V. , Simakin Alexander V. , Sarimov Ruslan M. , Kurilov Alexander D. , Chausov Denis N.
Published: October 2021

A technology for producing a nanocomposite based on the borsiloxane polymer and chemically unmodified fullerenes has been developed. Nanocomposites containing 0.001, 0.01, and 0.1 wt% fullerene molecules have been created. It has been shown that the nanocomposite with any content of fullerene molecules did not lose the main rheological properties of borsiloxane and is capable of structural self-healing. The resulting nanomaterial is capable of generating reactive oxygen species (ROS) such as hydrogen peroxide and hydroxyl radicals in light. The rate of ROS generation increases with an increase in the concentration of fullerene molecules. In the absence of light, the nanocomposite exhibits antioxidant properties. The severity of antioxidant properties is also associated with the concentration of fullerene molecules in the polymer. It has been shown that the nanocomposite upon exposure to visible light leads to the formation of long-lived reactive protein species, and is also the reason for the appearance of such a key biomarker of oxidative stress as 8-oxoguanine in DNA. The intensity of the process increases with an increase in the concentration of fullerene molecules. In the dark, the polymer exhibits weak protective properties. It was found that under the action of light, the nanocomposite exhibits significant bacteriostatic properties, and the severity of these properties depends on the concentration of fullerene molecules. Moreover, it was found that bacterial cells adhere to the surfaces of the nanocomposite, and the nanocomposite can detach bacterial cells not only from the surfaces, but also from wetted substrates. The ability to capture bacterial cells is primarily associated with the properties of the polymer; they are weakly affected by both visible light and fullerene molecules. The nanocomposite is non-toxic to eukaryotic cells, the surface of the nanocomposite is suitable for eukaryotic cells for colonization. Due to the combination of self-healing properties, low cytotoxicity, and the presence of bacteriostatic properties, the nanocomposite can be used as a reusable dry disinfectant, as well as a material used in prosthetics.
MoTe2 Field-Effect Transistors with Low Contact Resistance through Phase Tuning by Laser Irradiation
Authors: Bae Geun Yeol , Kim Jinsung , Kim Junyoung , Lee Siyoung , Lee Eunho
Published: October 2021

Due to their extraordinary electrical and physical properties, two-dimensional (2D) transition metal dichalcogenides (TMDs) are considered promising for use in next-generation electrical devices. However, the application of TMD-based devices is limited because of the Schottky barrier interface resulting from the absence of dangling bonds on the TMDs’ surface. Here, we introduce a facile phase-tuning approach for forming a homogenous interface between semiconducting hexagonal (2H) and semi-metallic monoclinic (1T′) molybdenum ditelluride (MoTe2). The formation of ohmic contacts increases the charge carrier mobility of MoTe2 field-effect transistor devices to 16.1 cm2 V−1s−1 with high reproducibility, while maintaining a high on/off current ratio by efficiently improving charge injection at the interface. The proposed method enables a simple fabrication process, local patterning, and large-area scaling for the creation of high-performance 2D electronic devices.
Construction of a Nano-Controlled Release Methotrexate Delivery System for the Treatment of Rheumatoid Arthritis by Local Percutaneous Administration
Authors: Guo Tingting , Kang Xu , Ren Sifan , Ouyang Xianjin , Chang Mingming
Published: October 2021

A drug delivery system was specifically designed for the treatment of rheumatoid arthritis (RA) by local percutaneous administration and the nano-controlled release of methotrexate (MTX). The release behavior of MTX from the synthesized MTX-mSiO2@PDA system was investigated in vitro and in vivo. The obtained results show that after 48 h, twice as much MTX (cumulative amount) is released at pH 5.5 than at pH 7.4. This suggests that the MTX-mSiO2@PDA system exhibits a good pH sensitivity. In vitro local percutaneous administration experiments revealed that the cumulative amount of MTX transferred from MTX-mSiO2@PDA to pH 5.0 receptor fluid through the whole skin was approximately three times greater than the amount transferred to pH 7.4 receptor fluid after 24 h. Moreover, in vivo experiments conducted on a complete induced arthritis (CIA) model in DBA/1 mice demonstrated that the thickness of a mouse’s toes decreases to nearly 65% of the initial level after 27 days of local percutaneous MTX-mSiO2@PDA administration. Compared to the mice directly injected with MTX, those administered with MTX-mSiO2@PDA by local percutaneous application exhibit much lower toe thickness deviation, which indicates that the latter group experiences a better cure stability. Overall, these results demonstrate that the local percutaneous administration of MTX delivery systems characterized by nano-controlled release may play an important role in RA therapy.
Impedance Spectroscopy Analysis of PbSe Nanostructures Deposited by Aerosol Assisted Chemical Vapor Deposition Approach
Authors: Iram Sadia , Mahmood Azhar , Ehsan Muhammad Fahad , Mumtaz Asad , Sohail Manzar , Sitara Effat , Mushtaq Shehla , Malik Mohammad Azad , Fatima Syeda Arooj , Shaheen Rubina , Ahmad Nasir Mahmood , Malik Sajid Nawaz
Published: October 2021

This research endeavor aimed to synthesize the lead (II) diphenyldiselenophosphinate complex and its use to obtain lead selenide nanostructured depositions and further the impedance spectroscopic analysis of these obtained PbSe nanostructures, to determine their roles in the electronics industry. The aerosol-assisted chemical vapor deposition technique was used to provide lead selenide deposition by decomposition of the complex at different temperatures using the glass substrates. The obtained films were revealed to be a pure cubic phase PbSe, as confirmed by X-ray diffraction analysis. SEM and TEM micrographs demonstrated three-dimensionally grown interlocked or aggregated nanocubes of the obtained PbSe. Characteristic dielectric measurements and the impedance spectroscopy analysis at room temperature were executed to evaluate PbSe properties over the frequency range of 100 Hz–5 MHz. The dielectric constant and dielectric loss gave similar trends, along with altering frequency, which was well explained by the Koops theory and Maxwell–Wagner theory. The effective short-range translational carrier hopping gave rise to an overdue remarkable increase in ac conductivity (σac) on the frequency increase. Fitting of a complex impedance plot was carried out with an equivalent circuit model (Rg Cg) (Rgb Qgb Cgb), which proved that grains, as well as grain boundaries, are responsible for the relaxation processes. The asymmetric depressed semicircle with the center lower to the impedance real axis provided a clear explanation of non-Debye dielectric behavior.
High Q Resonant Graphene Absorber with Lossless Phase Change Material Sb2S3
Authors: Meng Qi , Chen Xingqiao , Xu Wei , Zhu Zhihong , Qin Shiqiao , Zhang Jianfa , Yuan Xiaodong
Published: October 2021

Graphene absorbers have attracted lots of interest in recent years. They provide huge potential for applications such as photodetectors, modulators, and thermal emitters. In this letter, we design a high-quality (Q) factor resonant graphene absorber based on the phase change material Sb2S3. In the proposed structure, a refractive index grating is formed at the subwavelength scale due to the periodical distributions of amorphous and crystalline states, and the structure is intrinsically flat. The numerical simulation shows that nearly 100% absorption can be achieved at the wavelength of 1550 nm, and the Q factor is more than hundreds due to the loss-less value of Sb2S3 in the near-infrared region. The absorption spectra can be engineered by changing the crystallization fraction of the Sb2S3 as well as by varying the duty cycle of the grating, which can be employed not only to switch the resonant wavelength but also to achieve resonances with higher Q factors. This provides a promising method for realizing integrated graphene optoelectronic devices with the desired functionalities.
High-Quality Graphene-Based Tunable Absorber Based on Double-Side Coupled-Cavity Effect
Authors: Wang Qiong , Ouyang Zhengbiao , Lin Mi , Zheng Yaoxian
Published: October 2021

Graphene-based devices have important applications attributed to their superior performance and flexible tunability in practice. In this paper, a new kind of absorber with monolayer graphene sandwiched between two layers of dielectric rings is proposed. Two peaks with almost complete absorption are realized. The mechanism is that the double-layer dielectric rings added to both sides of the graphene layer are equivalent to resonators, whose double-side coupled-cavity effect can make the incident electromagnetic wave highly localized in the upper and lower surfaces of graphene layer simultaneously, leading to significant enhancement in the absorption of graphene. Furthermore, the influence of geometrical parameters on absorption performance is investigated in detail. Also, the device can be actively manipulated after fabrication through varying the chemical potential of graphene. As a result, the frequency shifts of the two absorption peaks can reach as large as 2.82 THz/eV and 3.83 THz/eV, respectively. Such a device could be used as tunable absorbers and other functional devices, such as multichannel filters, chemical/biochemical modulators and sensors.
Thickness Dependence of Superconductivity in Layered Topological Superconductor β-PdBi2
Authors: Li Huijie , Wang Huanhuan , Gao Wenshuai , Chen Zheng , Han Yuyan , Zhu Xiangde , Tian Mingliang
Published: October 2021

We report a systematic study on the thickness-dependent superconductivity and transport properties in exfoliated layered topological superconductor β-PdBi2. The superconducting transition temperature Tc is found to decrease with the decreasing thickness. Below a critical thickness of 45 nm, the superconductivity is suppressed, but followed by an abrupt resistance jump near Tc, which is in opposite to the behavior in a superconductor. We attribute suppressed Tc to the enhanced disorder as the thickness decreases. The possible physical mechanisms were discussed for the origination of sharply increased resistance in thinner β-PdBi2 samples.
How Does the Family Influence Adolescent Eating Habits in Terms of Knowledge, Attitudes and Practices? A Global Systematic Review of Qualitative Studies
Authors: Liu Kiki S. N. , Chen Julie Y. , Ng Michelle Y. C. , Yeung Maegan H. Y. , Bedford Laura E. , Lam Cindy L. K.
Published: October 2021

Promoting healthy eating habits can prevent adolescent obesity in which family may play a significant role. This review synthesized findings from qualitative studies to identify family barriers and facilitators of adolescent healthy eating in terms of knowledge, attitudes, and practices (KAP). A literature search of four databases was completed on 31 July 2020; qualitative studies that explored family factors of adolescent (aged 10 to 19 years) eating habits were included. A total of 48 studies were identified, with the majority being from North America and sampled from a single source. Ten themes on how family influences adolescent dietary KAP were found: Knowledge—(1) parental education, (2) parenting style, and (3) family illness experience; Attitudes—(4) family health, (5) cultivation of preference, and (6) family motivation; Practices—(7) home meals and food availability, (8) time and cost, (9) parenting style, and (10) parental practical knowledge and attitudes. This review highlights five parental characteristics underlying food parenting practices which affect adolescents’ KAP on healthy eating. Adolescents with working parents and who are living in low-income families are more vulnerable to unhealthy eating. There is a need to explore cultural-specific family influences on adolescents’ KAP, especially regarding attitudes and food choices in Asian families.
Psychophysiological Effects of Lactobacillus plantarum PS128 in Patients with Major Depressive Disorder: A Preliminary 8-Week Open Trial
Authors: Chen Hui-Mei , Kuo Po-Hsiu , Hsu Chia-Yueh , Chiu Yi-Hung , Liu Yen-Wenn , Lu Mong-Liang , Chen Chun-Hsin
Published: October 2021

Recent studies have suggested that gut–brain axis may be one of the mechanisms of major depression disorder (MDD). The current study aimed to investigate the effects of Lactobacillus plantarum PS128 (PS128) on psychophysiology in patients with MDD. We recruited 11 patients with MDD and gave them PS128 for 8 weeks. We compared depression symptoms, serum markers of inflammation and gut permeability, and gut microbiota before and after 8-week intervention and also explored the correlations among symptoms, biomarkers, and gut microbiota. After 8-week PS128 intervention, scores of Hamilton Depression Rating Scale-17 and Depression and Somatic symptoms Scale significantly decreased. Serum levels of high sensitivity c-reactive protein, interluekin-6, and tumor necrosis factor-α, zonulin and intestinal fatty acid binding protein, and the composition of gut microbiota did not significantly change after 8-week PS128 intervention. However, we found changes of some genera were correlated with changes of symptoms and biomarkers. In conclusion, this is an open trial with small sample size and has several limitations. The results need to be verified by randomized, double-blind, placebo-controlled trial with larger sample size.
Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis—Mechanistic Insights into Anemia of Inflammation and Its Treatment
Authors: Lanser Lukas , Fuchs Dietmar , Kurz Katharina , Weiss Günter
Published: October 2021

Anemia is very common in patients with inflammatory disorders. Its prevalence is associated with severity of the underlying disease, and it negatively affects quality of life and cardio-vascular performance of patients. Anemia of inflammation (AI) is caused by disturbances of iron metabolism resulting in iron retention within macrophages, a reduced erythrocyte half-life, and cytokine mediated inhibition of erythropoietin function and erythroid progenitor cell differentiation. AI is mostly mild to moderate, normochromic and normocytic, and characterized by low circulating iron, but normal and increased levels of the storage protein ferritin and the iron hormone hepcidin. The primary therapeutic approach for AI is treatment of the underlying inflammatory disease which mostly results in normalization of hemoglobin levels over time unless other pathologies such as vitamin deficiencies, true iron deficiency on the basis of bleeding episodes, or renal insufficiency are present. If the underlying disease and/or anemia are not resolved, iron supplementation therapy and/or treatment with erythropoietin stimulating agents may be considered whereas blood transfusions are an emergency treatment for life-threatening anemia. New treatments with hepcidin-modifying strategies and stabilizers of hypoxia inducible factors emerge but their therapeutic efficacy for treatment of AI in ill patients needs to be evaluated in clinical trials.
Role of CD38 in Adipose Tissue: Tuning Coenzyme Availability?
Authors: Benzi Andrea , Grozio Alessia , Spinelli Sonia , Sturla Laura , Guse Andreas H. , De Flora Antonio , Zocchi Elena , Heeren Joerg , Bruzzone Santina
Published: October 2021

Nicotinamide adenine dinucleotide (NAD+) is a fundamental molecule in the regulation of energy metabolism, representing both a coenzyme and a substrate for different NAD+ degrading enzymes. Among these enzymes, CD38 can be seen under two perspectives: as the enzyme synthesizing Ca2+-mobilizing second messenger, starting from NAD+, and as the major NAD+-consumer, to be inhibited to increase NAD+ levels. Indeed, the regulation of NAD+ availability is a key event during different processes. In this review, we examine the recent studies related to the modulation of CD38 expression and activity, and the consequent changes in NAD(P)(H), in adipose tissue, during inflammation and cold-induced thermogenesis.
Food and Grain Consumption Per Capita in the Qinghai–Tibet Plateau and Implications for Conservation
Authors: Wang Lijing , Xiao Yi , Ouyang Zhiyun
Published: October 2021

Background: Grain security is crucial for social stability and ecosystem conservation regionally and globally, and it is particularly concerned widely in the Qinghai-Tibet Plateau (QTP) due to its high altitude and harsh climate for agriculture. Method: In this paper, we calculated and analyzed per capita food and grain consumption, including direct grain consumption, grain for fodder, industry consumption, seeds consumption, and wastage consumption and its changes in the QTP during 1995–2019. Results: The results showed that (1) in 2019, the average food consumption per capita was 333.35 kg, was stable since 1995. The dietary structure of residents was composed of direct grain consumption (44.15%), meat (10.72%), and milk (6.94%). The consumption of meat and milk was higher than the national average. (2) The average daily intake of energy and protein, animal protein, and the ratio of high-quality protein and fat energy were 2156.21 kcal·d−1, 73.53 g·d−1, 23.06 g·d−1, 38.32%, and 27.77% in 2019. Their changes were −342.98 kcal·d−1, −8.91 g·d−1, 11.16 g·d−1, 18.37%, and 11.08%, respectively. (3) The corresponding grain consumption per capita was 284.90 kg·a−1 in 1995, 262.19 kg·a−1 in 2010, and then remained stable until 2019. Conclusion: The study suggested that food consumption per capital was guaranteed at the well-off level since 2010, and food and dietary structure of residents were corresponding to physical geographic and climatic environment in the QTP. The conflict between food security and the ecosystem conservation can be managed without scarifying nature as the total grain consumption was stable since 2010, and the yield per unit area and total grain yield were both increasing since 2003 for agricultural condition improved in the QTP.
Trajectories of Food Choice Motives and Weight Status of Malaysian Youths during the COVID-19 Pandemic
Authors: Tan Seok Tyug , Tan Chin Xuan , Tan Seok Shin
Published: October 2021

Stay-at-home orders have abruptly altered food purchasing behaviour, dietary habits, and food choice motives. Therefore, this study aims to investigate the trajectory of food choice motives and their associations with the weight status of Malaysian youths in the time of COVID-19. Socio-demographic information and anthropometric measurements were self-reported by the respondents, while the food choice motives were assessed using a validated 38-item food choice questionnaire (FCQ). Of the 1013 Malaysian youths, 48.6% gained weight due to the confinement, with an average weight gain of 3.90 ± 2.92 kg. On the other hand, 47.0% to 73.0% of the youths changed their food choice motives in the time of COVID-19. Of the 10 motives, convenience (48.5%) had the largest percentage increase, followed by weight control (47.0%) and health (45.3%). Moreover, the mean scores of health (t = −3.324, p = 0.001), convenience (t = −5.869, p < 0.001), weight control (t = −7.532, p < 0.001), natural content (t = −5.957, p < 0.001), ethical concern (t = −4.419, p < 0.001) and price (t = −3.737, p < 0.001) were significantly higher during the pandemic compared to pre-pandemic. Findings from the multinomial regression model revealed that youths highly concerned for weight control were more likely to be in the weight loss category (Adjusted odds ratio (AOR) = 1.633, Confidence Interval (CI) = 1.230–2.168, p = 0.001). Conversely, those who gained weight due to the pandemic confinement highly valued natural content in foods (AOR = 0.653, CI = 0.481–0.886, p = 0.006) when making their food choices in this unprecedented pandemic. In conclusion, Malaysian youths made healthier food choices to mitigate the risk of COVID-19 infection.
Efficacy of Creatine Supplementation Combined with Resistance Training on Muscle Strength and Muscle Mass in Older Females: A Systematic Review and Meta-Analysis
Authors: dos Santos Ellem Eduarda Pinheiro , de Araújo Rodrigo Cappato , Candow Darren G. , Forbes Scott C. , Guijo Jaddy Antunes , de Almeida Santana Carla Caroliny , Prado Wagner Luiz do , Botero João Paulo
Published: October 2021

Sarcopenia refers to the age-related loss of muscle strength and muscle mass, which is associated with a reduced quality of life, particularly in older females. Resistance training (RT) is well established to be an effective intervention to counter indices of sarcopenia. Accumulating research indicates that the addition of creatine supplementation (Cr) to RT augments gains in muscle strength and muscle mass, compared to RT alone. However, some evidence indicates that sex differences may alter the effectiveness of Cr. Therefore, we systematically reviewed randomized controlled trials (RCTs) investigating the efficacy of Cr + RT on measures of upper- and lower-body strength and muscle mass in older females. A systematic literature search was performed in nine electronic databases. Ten RCTs (N = 211 participants) were included the review. Overall, Cr significantly increased measures of upper-body strength (7 studies, n = 142, p = 0.04), with no effect on lower-body strength or measures of muscle mass. Sub-analyses revealed that both upper-body (4 studies, n = 97, p = 0.05) and lower-body strength (4 studies, n = 100, p = 0.03) were increased by Cr, compared to placebo in studies ≥ 24 weeks in duration. In conclusion, older females supplementing with Cr experience significant gains in muscle strength, especially when RT lasts for at least 24 weeks in duration. However, given the level of evidence, future high-quality studies are needed to confirm these findings.
Supplementation with Spirulina platensis Prevents Uterine Diseases Related to Muscle Reactivity and Oxidative Stress in Rats Undergoing Strength Training
Authors: Ferreira Paula Benvindo , Diniz Anderson Fellyp Avelino , Lacerda Júnior Francisco Fernandes , Silva Maria da Conceição Correia , Cardoso Glêbia Alexa , Silva Alexandre Sérgio , da Silva Bagnólia Araújo
Published: October 2021

Strength training increases systemic oxygen consumption, causing the excessive generation of reactive oxygen species, which in turn, provokes oxidative stress reactions and cellular processes that induce uterine contraction. The aim of this study was to evaluate the possible protective effect of Spirulina platensis (SP), an antioxidant blue algae, on the contractile and relaxation reactivity of rat uterus and the balance of oxidative stress/antioxidant defenses. Female Wistar rats were divided into sedentary (CG), trained (TG), and T + supplemented (TG50, TG100) groups. Reactivity was analyzed by AQCAD, oxidative stress was evaluated by the malondialdehyde (MDA) formation, and the antioxidant capacity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Strength training increased contractile reactivity and decreased the pharmaco-mechanical component of relaxing reactivity in rat uterus. In addition, training decreased oxidation inhibition in the plasma and exercise increased oxidative stress in the uterine tissue; however, supplementation with algae prevented this effect and potentiated the increase in antioxidant capacity. Therefore, this study demonstrated that food supplementation prevents changes in reactivity and oxidative stress induced by strength training in a rat uterus, showing for the first time, that the uterus is a target for this exercise modality and antioxidant supplementation with S. platensis is an alternative means of preventing uterine dysfunction.
Highly Variable Clinical Pictures in Dogs Naturally Infected with Angiostrongylus vasorum
Authors: Colombo Mariasole , Traversa Donato , Grillotti Eleonora , Pezzuto Carlo , De Tommaso Cesare , Pampurini Fabrizio , Schaper Roland , Drake Jason , Crisi Paolo Emidio , Russi Ilaria , Ripamonti Marco , Di Cesare Angela
Published: October 2021

Canine angiostrongylosis by Angiostrongylus vasorum is increasingly reported in both enzootic and previously free areas. The complex pathogenesis of the disease makes the clinical workup challenging. Infected dogs show highly variable clinical pictures, characterized by subclinical to life-threatening general, cardio-respiratory, neurological and/or gastrointestinal signs. The present study reports the high variability of clinical pictures from 36 dogs across central and southern Italy that were naturally infected by A. vasorum. Of them, 23 (63.9%) presented at least one clinical sign, while 13 (36.1%) were subclinically infected and apparently healthy. Overall, 19 dogs (52.8%) showed cardiorespiratory signs, 14 (38.9%) had non-specific abnormalities, 2 (5.6%) presented coagulation disorders and 1 (2.8%) had a severe neurological condition. Importantly, four dogs presenting with clinical signs had neither cough nor dyspnea. These results underline that angiostrongylosis should be included in the differential diagnosis, even when dogs display only non-specific clinical signs. The proportion of apparently healthy dogs highlights the relevance of routine copromicroscopic and/or antigenic tests in enzootic areas to avoid the sudden onset of potentially life-threatening signs.
L-Lysine α-Oxidase: Enzyme with Anticancer Properties
Authors: Lukasheva Elena V. , Babayeva Gulalek , Karshieva Saida Sh. , Zhdanov Dmitry D. , Pokrovsky Vadim S.
Published: October 2021

L-lysine α-oxidase (LO), one of L-amino acid oxidases, deaminates L-lysine with the yield of H2O2, ammonia, and α-keto-ε-aminocaproate. Multiple in vitro and in vivo studies have reported cytotoxic, antitumor, antimetastatic, and antitumor activity of LO. Unlike asparaginase, LO has a dual mechanism of action: depletion of L-lysine and formation of H2O2, both targeting tumor growth. Prominent results were obtained on murine and human tumor models, including human colon cancer xenografts HCT 116, LS174T, and T47D with maximum T/C 12, 37, and 36%, respectively. The data obtained from human cancer xenografts in immunodeficient mice confirm the potential of LO as an agent for colon cancer treatment. In this review, we discuss recently discovered molecular mechanisms of biological action and the potential of LO as anticancer enzyme.
Synthesis and Biodistribution of 99mTc-Labeled PLGA Nanoparticles by Microfluidic Technique
Authors: Varani Michela , Campagna Giuseppe , Bentivoglio Valeria , Serafinelli Matteo , Martini Maria Luisa , Galli Filippo , Signore Alberto
Published: October 2021

The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with 99mTc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and >80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with 99mTc using the microfluidics and provide best formulation for tumor targeting studies.
Wide-Field Telescope Alignment Using the Model-Based Method Combined with the Stochastic Parallel Gradient Descent Algorithm
Authors: Li Min , Zhang Ang , Zhang Junbo , Xian Hao
Published: October 2021

To acquire images with higher accuracy of wide-field telescopes, deformable mirrors with more than 100 actuators are used, making the telescope alignment more complex and time-consuming. Furthermore, the position of the obscuration caused by the secondary mirror in the experiment system is changed with the difference of fields of view, making the response matrix of the deformable mirror different in various fields of view. To solve this problem, transfer functions corresponding to different fields of view are calculated according to the wavefront edge check and boundary conditions. In this paper, a model-based method combined with the stochastic parallel gradient descent (SPGD) algorithm is used. The experiment results show that our method can correct the aberrations with a high accuracy in both on-axis and off-axis fields, indicating that the effective actuators are well chosen corresponding to different fields of view.
Refractive Index Sensing Based on Multiple Fano Resonances in a Split-Ring Cavity-Coupled MIM Waveguide
Authors: Chen Jianfeng , Yang Hao , Fang Zhiyuan , Zhao Ming , Xie Chenbo
Published: October 2021

A metal–insulator–metal (MIM) waveguide consisting of a circular split-ring resonance cavity (CSRRC) and a double symmetric rectangular stub waveguide (DSRSW) is designed, which can excite quadruple Fano resonances. The finite element method (FEM) is used to investigate influences of geometric parameters on the transmission characteristics of the structure. The results show that Fano resonances are excited by the interference between the DSRSW and the CSRRC. Among them, the resonance wavelengths of the Fano resonances are tuned by the narrow-band discrete state excited by the CSRRC, and the resonance line transmittance and profiles are tuned by the wide-band continuous state excited by the DSRSW. The sensitivity (S) can be up to 1328.8 nm/RIU, and the figure of merit (FOM) can be up to 4.80 × 104. Based on these advantages, the structure has potential applications in sensing in the sub-wavelength range.
The Generic Risks and the Potential of SDN-1 Applications in Crop Plants
Authors: Kawall Katharina
Published: October 2021

The use of site-directed nucleases (SDNs) in crop plants to alter market-oriented traits is expanding rapidly. At the same time, there is an on-going debate around the safety and regulation of crops altered with the site-directed nuclease 1 (SDN-1) technology. SDN-1 applications can be used to induce a variety of genetic alterations ranging from fairly ‘simple’ genetic alterations to complex changes in plant genomes using, for example, multiplexing approaches. The resulting plants can contain modified alleles and associated traits, which are either known or unknown in conventionally bred plants. The European Commission recently published a study on new genomic techniques suggesting an adaption of the current GMO legislation by emphasizing that targeted mutagenesis techniques can produce genomic alterations that can also be obtained by natural mutations or conventional breeding techniques. This review highlights the need for a case-specific risk assessment of crop plants derived from SDN-1 applications considering both the characteristics of the product and the process to ensure a high level of protection of human and animal health and the environment. The published literature on so-called market-oriented traits in crop plants altered with SDN-1 applications is analyzed here to determine the types of SDN-1 application in plants, and to reflect upon the complexity and the naturalness of such products. Furthermore, it demonstrates the potential of SDN-1 applications to induce complex alterations in plant genomes that are relevant to generic SDN-associated risks. In summary, it was found that nearly half of plants with so-called market-oriented traits contain complex genomic alterations induced by SDN-1 applications, which may also pose new types of risks. It further underscores the need for data on both the process and the end-product for a case-by-case risk assessment of plants derived from SDN-1 applications.
The Efficiency of Different Priming Agents for Improving Germination and Early Seedling Growth of Local Tunisian Barley under Salinity Stress
Authors: Ben Youssef Rim , Jelali Nahida , Boukari Nadia , Albacete Alfonso , Martinez Cristina , Alfocea Francisco Perez , Abdelly Chedly
Published: October 2021

The current work aimed to investigate the effect of seed priming with different agents (CaCl2, KCl, and KNO3) on germination and seedling establishment in seeds of the barley species of both Hordeum vulgare (L. Manel) and Hordeum maritimum germinated with three salt concentrations (0, 100, and 200 mM NaCl). The results showed that under unprimed conditions, salt stress significantly reduced the final germination rate, the mean daily germination, and the seedling length and dry weight. It led to a decrease in the essential nutrient content (iron, calcium, magnesium, and potassium) against an increase in sodium level in both of the barley species. Moreover, this environmental constraint provoked a membrane injury caused by a considerable increase in electrolyte leakage and the malondialdehyde content (MDA). Data analysis proved that seed priming with CaCl2, KCl, and KNO3 was an effective method for alleviating barley seed germination caused by salt stress to varying degrees. Different priming treatments clearly stimulated germination parameters and the essential nutrient concentration, in addition to increasing the seedling growth rate. The application of seed priming reduced the accumulation of sodium ions and mitigated the oxidative stress of seeds caused by salt. This mitigation was traduced by the maintenance of low levels of MDA and electrolyte leakage. We conclude that the priming agents can be classed into three ranges based on their efficacy on the different parameters analyzed; CaCl2 was placed in the first range, followed closely by KNO3, while the least effective was KCl, which placed in the third range.
The Function of Flavonoids in the Diurnal Rhythm under Rapidly Changing UV Conditions—A Model Study on Okra
Authors: Neugart Susanne , Tobler Mark A. , Barnes Paul W.
Published: October 2021

Flavonoids are favored compounds in plant responses to UV exposure and act in UV absorption and antioxidant activity. Here, it was investigated, with okra as a model species, how fast plants can react to changing UV conditions and to what extent these reactions take place. Okra (Abelmoschus esculentus) plants were exposed to either full or nearly no UV radiation. The diurnal rhythm of the plants was driven by the UV radiation and showed up to a 50% increase of the flavonoid content (measured optically in the +UV plants). This was reflected only in the trends in UV-absorption and antioxidant activity of the extracts but not in the soluble flavonoid glycosides and hydroxycinnamic acid derivatives. In a second experiment, a transfer from a −UV to a +UV condition at 9:00 CDT showed the immediate start of the diurnal rhythm, while this did not occur if the transfer occurred later in the day; these plants only started a diurnal rhythm the following day. After an adaptation period of seven days, clear differences between the +UV and -UV plants could be found in all parameters, whereas plants transferred to the opposite UV condition settle between the +UV and -UV plants in all parameters. Broadly, it can be seen that the flavonoid contents and associated functions in the plant are subject to considerable changes within one day and within several days due to the UV conditions and that this can have a considerable impact on the quality of plant foods.
Effect of Silicone Inlaid Materials on Reinforcing Compressive Strength of Weft-Knitted Spacer Fabric for Cushioning Applications
Authors: Yu Annie , Sukigara Sachiko , Shirakihara Miwa
Published: October 2021

Spacer fabrics are commonly used as cushioning materials. They can be reinforced by using a knitting method to inlay materials into the connective layer which reinforces the structure of the fabric. The compression properties of three samples that were fabricated by inlaying three different types of silicone-based elastic tubes and one sample without inlaid material have been investigated. The mechanical properties of the elastic tubes were evaluated and their relationship to the compression properties of the inlaid spacer fabrics was analysed. The compression behaviour of the spacer fabrics at an initial compressive strain of 10% is not affected by the presence of the inlaid tubes. The Young’s modulus of the inlaid tubes shows a correlation with fabric compression. Amongst the inlaid fabric samples, the spacer fabric inlaid with highly elastic silicone foam tubes can absorb more compression energy, while that inlaid with silicone tubes of higher tensile strength has higher compressive stiffness.
Influence of Carbon Nanotubes Concentration on Mechanical and Electrical Properties of Poly(styrene-co-acrylonitrile) Composite Yarns Electrospun
Authors: Caro-Briones Rubén , García-Pérez Blanca Estela , Martín-Martínez Eduardo San , Báez-Medina Héctor , Cruz-Reyes Irlanda Grisel , del Río José Manuel , Martínez-Gutiérrez Hugo , Corea Mónica
Published: October 2021

In this work, the influence of carbon nanotubes (CNTs) content on the mechanical and electrical properties of four series of polymeric matrix were made and their cytotoxicity on cells was evaluated to consider their use as a possible artificial muscle. For that, polymer composite yarns were electrospun using polymeric solutions at 10 wt.%. of poly(styrene-co-acrylonitrile) P(S:AN) and P(S:AN-acrylic acid) P(S:AN-AA) at several monomeric concentrations, namely 0:100, 20:80, 40:60, 50:50 (wt.%:wt.%), and 1 wt.% of AA. Carbon nanotubes (CNTs) were added to the polymeric solutions at two concentrations, 0.5 and 1.0 wt.%. PMCs yarns were collected using a blade collector. Mechanical and electrical properties of polymeric yarns indicated a dependence of CNTs content into yarns. Three areas could be found in fibers: CNTs bundles zones, distributed and aligned CNTs zones, and polymer-only zones. PMCs yarns with 0.5 wt.% CNTs concentration were found with a homogenous nanotube dispersion and axial alignment in polymeric yarn, ensuring load transfer on the polymeric matrix to CNTs, increasing the elastic modulus up to 27 MPa, and a maximum electrical current of 1.8 mA due to a good polymer–nanotube interaction.
Peculiarities of Oxidative Polymerization of Diarylaminodichlorobenzoquinones
Authors: Orlov Andrey V. , Kiseleva Svetlana G. , Karpacheva Galina P. , Muratov Dmitriy G.
Published: October 2021

New oxidative polymerization monomers—diarylaminodichlorobenzoquinones were synthesised by alkylating aniline, m-phenylenediamine and methanilic acid with chloranil. Oxidative polymerization of diarylaminodichlorobenzoquinones was studied for the first time in relation to the concentration of the monomer, acid, and oxidant/monomer ratio. It was found that the synthesized monomers are highly active in the polymerization reaction, and the oxidation rate grows with the increase in the acid concentration. Only one arylamine group is involved in the polymerization reaction. The optimal oxidant/monomer ratio is stoichiometric for one arylamine group, despite the bifunctionality of the monomers. It was shown that the type of the substituent in the aniline ring (electron donor or electron acceptor) determines the growth of the polymer chain and the structure of the resulting conjugated polymers. A mechanism for the formation of active polymerization centers for diarylaminodichlorobenzoquinones was proposed. FTIR-, NMR-, X-ray photoelectron spectroscopy, and SEM were used to identify the structure of the synthesized monomers and polymers. The obtained polymers have an amorphous structure and a loose globular morphology. The frequency dependence of the electrical conductivity was studied.
Searching Nanoplastics: From Sampling to Sample Processing
Authors: Cerasa Marina , Teodori Simona , Pietrelli Loris
Published: October 2021

Nanoplastics (NPs) are considered emerging pollutants, namely unregulated contaminants whose toxic effect on humans and the environment has been demonstrated or suspected. They are the result of the physical fragmentation of the plastics that over time reach smaller dimensions (<100 nm). The issues related to the characterization and quantification of NPs in the environmental matrices are mainly related to the infinitepsimal size, to the fact that they are found in bulk, and to the different physico-chemical forms in which the same polymer can evolve over time by degradation. To deal with the study of a new class of pollutants it is necessary to assess the entire analytical method, carefully considering every single step (sampling, cleanup, qualitative, and quantitative analysis) starting from the validation method in the laboratory. This paper reviews the analytical method steps, focusing on the first ones, which the current literature often underestimates: laboratory tests, sampling, and sample processing; in fact, most errors and the quality of the analyses often depend on them. In addition, all newly introduced sample processing methods were examined.
Biotechnological Addition of β-Glucans from Cereals, Mushrooms and Yeasts in Foods and Animal Feed
Authors: Chiozzi Viola , Eliopoulos Christos , Markou Giorgos , Arapoglou Dimitrios , Agriopoulou Sofia , El Enshasy Hesham A. , Varzakas Theodoros
Published: October 2021

Varied cereal plants including, mushrooms, yeast, bacteria and algae are important sources of β-glucans, and many extraction procedures have been used in order to recover these valuable naturally occurring polysaccharides. The rheological and molecular properties of β-glucans can be utilized to be incorporated into various foods and to offer properties extremely beneficial to human health. Their functional effects are mainly determined by their molecular and structural characteristics. Consumption of foods fortified and enriched with β-glucans can contribute to the treatment of certain chronic diseases. Reduced cholesterol, cardiovascular and diabetic risk and moderate glycemic response of foods have been recorded with the consumption of these biologically active compounds. In addition, β-glucans are characterized by anti-cancer, antioxidant, anti-inflammatory and antiviral activities. As β-glucans interact with the foods in which they are incorporated, this review aims to discuss recent applications with quality and nutritional results of β-glucans incorporation with foods such as beverages, dairy, bakery, meat and pasta products, as well as their addition in animal feeds and their uses in other fields such as medicine.
Process-Induced Modifications on Quality Attributes of Cassava (Manihot esculenta Crantz) Flour
Authors: Udoro Elohor Oghenechavwuko , Anyasi Tonna Ashim , Jideani Afam Israel Obiefuna
Published: October 2021

Cassava flour (CF) is a suitable representative and one of the easiest shelf-stable food products of the edible portion of the highly perishable cassava root (Manihot esculenta Crantz). The quality and type of CF are dependent on processing variables. Broadly categorized into fermented and unfermented CF, unfermented CF is white, odorless, and bland, while fermented CF has a sour flavor accompanied by its characteristic odor. The use of fermented CF as a composite is limited because of their off-odors. Modifications in CF processing have given rise to prefixes such as: modified, unmodified, gelatinized, fortified, native, roasted, malted, wet, and dry. Consumed alone, mostly in reconstituted dough form with soups, CF may also serve as a composite in the processing of various flour-based food products. Fermenting with microorganisms such as Rhizopus oryzae and Saccharomyces cerevisiae results in a significant increase in the protein content and a decrease in the cyanide content of CF. However, there are concerns regarding its safety for consumption. Pre-gelatinized CF has potential for the textural and structural improvement of bakery products. The average particle size of the CF also influences its functional properties and, subsequently, the quality of its products. Cassava flour is best stored at ambient temperature. Standardizing the processing of CF is a challenge because it is mostly processed in artisanal units. Furthermore, each variety of the root best suits a particular application. Therefore, understanding the influence of processing variables on the characteristics of CF may improve the utilization of CF locally and globally.