bluuebunny/crossref_metadata_2025 huggingface dataset:
Geological and geomorphological evidence of holocene activity along the maisu fault in the northern Sichuan–Yunnan block, Tibetan Plateau
Authors: Zuo Hong , Qin Yulong , Liang Mingjian , Sun Kai , Huang Feipeng , Liao Cheng , Zhou Wenying , Wu Weiwei , Zhang Huiping , Yang Yao
Published: September 2022

The Sichuan–Yunnan block is located in the eastern of Tibetan Plateau and exhibits strong tectonic and earthquake activity. The Maisu fault is an E–W-trending fault within this block. Via interpretations of remote-sensing imagery and field surveys, we identified a earthquake surface rupture zone that has developed along the Maisu fault; we then estimated its Holocene activity. The surface rupture extends westward from the town of Puma, Sichuan Province, to the village of Worilong, Xizang Province, and has a length of approximately 45 km. According to a fault outcrop and carbon-14 dating of a profile near the village of Yongqu, Xizang Province, the most recent earthquake along this rupture may have occurred after 1850 ± 30 BP. The Maisu fault extends eastward and may intersect the Garzê–Yushu fault. Accordingly, as a secondary fault, the Maisu fault likely accommodates the partitioned horizontal slip deformation of the Garzê–Yushu fault.
Effect of large topography on atmospheric environment in Sichuan Basin: A climate analysis based on changes in atmospheric visibility
Authors: Zhang Lei , Guo Xiaomei , Zhao Tianliang , Xu Xiangde , Zheng Xiaobo , Li Yueqing , Luo Lei , Gui Ke , Zheng Yu , Shu Zhuozhi
Published: September 2022

Using 51 years (1960–2010) of observations from meteorological stations in the Sichuan-Chongqing region, including atmospheric visibility as a proxy for aerosol concentration, relative humidity, air temperature, wind speed and FNL reanalysis data (1°×1°) of air temperature, pressure and wind, and the altitude of each station, a linear trend and multivariate fitting approach was used to explore the effects of the large topography on the atmospheric environment in the Sichuan-Chongqing region. The region mainly consists of two areas: Sichuan Basin (SCB) and Western Sichuan Plateau (WSP; eastern part of the Tibetan Plateau). Visibility was relatively low in the SCB and high in the WSP, indicating the high and low levels of aerosols respectively in the SCB and the WSP. Additionally, visibility and wind speed were positively correlated within the basin (altitude below 750 m), while negatively correlated at stations above 1,500 m, such as on the WSP, indicating that the topography had an influence on the atmospheric environment of the basin. On the one hand, the vertical structures of the wind fields and the vertical profiles of latitudinal deviations in wind speed and air temperature in the basin show that the unique large topography causes a “harbour” effect on the leeward-slope of the WSP, with the SCB being a weak wind region, while the descending air currents in the upper westerlies of the basin form a huge “vault” of air. On the other hand, topographic effects can make the basin more susceptible to the formation of inversion structures near the surface and at high altitudes, thus stabilising the atmosphere. The topographic effects, which is not conducive to horizontal diffusion and convective transport of pollutants, were the most significant in winter, followed by autumn and spring.
Mechanisms of Kaempferol in the treatment of diabetes: A comprehensive and latest review
Authors: Yang Yan , Chen Zhengtao , Zhao Xiaoyan , Xie Hongyan , Du Lian , Gao Hong , Xie Chunguang
Published: September 2022

Obesity–insulin resistance–β-cells apoptosis” is an important trilogy of the pathogenesis of type 2 diabetes. With the global pandemic of obesity and diabetes, continuous research and development of new drugs focuses on the prevention of the pathological progress of these diseases. According to a recent study, the natural product kaempferol has excellent antidiabetic effects. Therefore, this review comprehensively summarized the frontier studies and pharmacological mechanisms of kaempferol in the treatment of diabetes. The successful research and development of kaempferol may yield a significant leap in the treatment of diabetes and its complications.
A secure edge power system based on a Docker container
Authors: Xu Xinchen , Jiang Yixin , Wen Hong , Hou Wenjing , Chen Songlin
Published: September 2022

Thanks to the advantages of low latency, high efficiency, and oneself-security, the edge computing (EC) paradigm is expected to widely be applied in a large number of scenarios. However, because EC equipment is closer to a wide variety of terminals in a realistic environment, it is also more vulnerable to terminal attacks. The security of edge computing equipment itself cannot be ignored. Docker is a lightweight container technology that closely fits the existing needs of edge computing for portability, security isolation, and convenience. In this paper, Docker technology is taken as an edge computing security support engine and a security monitoring system based on the Docker container is built. In addition, combined with container monitoring and objective weighting method, a node security judgment method is proposed. Finally, according to the results of the node security judgment, a method for evaluating the security of the unmonitored node is put forward. Consequently, an edge computing security monitoring system based on the Docker container is built, and its performance in the real environment of a smart grid system is implemented to verify the proposed method. The results prove the efficiency and security protection of the novel edge power system.
Use of COVID-19 personal protective equipment pollutants to improve physical properties of silty sand in Yellow River flooded area
Authors: Wang Hao , Guo Jianbo , Liu Weiwei , Song Haoming , Yan Tiantian , Fu Dewei
Published: September 2022

Silty sand in the Yellow River flood area (YRFA) of China exhibits a low cohesive force and water-holding capacity. Its direct use for subgrade filling leads to phenomena such as subgrade bed depression, slurry, and mud. Therefore, from the perspective of waste utilization, this study investigates disposable protective clothing (DPC) and disposable nitrile gloves (DNG) produced due to COVID-19 in combination with silty sand as subgrade filling for the YRFA. Through an unconfined compressive strength and permeability test, we studied the influence of personal protective equipment (PPE) with different moisture content, concentration, and size on the strength and permeability of mixed samples. Further, we discuss its impact on the improvement of subgrade filling in YRFA. Results show that adding DPC improves both the compressive strength and ductility of the sample. However, the impermeability of the sample remains unchanged. In contrast, the addition of DNG does not increase the compressive strength and ductility of the sample at the same time, whereas it significantly improves its impermeability. The size of PPE has a considerable influence on the compressive strength of the mixed sample, whereas it does not affect the sample permeability. This method makes use of the PPE waste produced due to COVID-19, reduces environmental pollution, and provides a novel concept for improving silty sand in the YRFA.
Characteristics of loess landslides triggered by the 1927 Mw8.0 earthquake that occurred in Gulang County, Gansu Province, China
Authors: Zhong Xiumei , Xu Xiaowei , Chen Wenkai , Liang Yuxin , Sun Qinyao
Published: September 2022

The 1927 Gulang Mw8.0 earthquake was one of the largest earthquakes in Gansu, China, which triggered lots of loess landslides. However, the loess of the study area is atypical and different from that of the Loess Plateau. Meanwhile, there are few systematic research studies on the characteristics of these seismic landslides. Combined with previous studies and field investigations, the landslide distribution has been revealed through the visual interpretation of remote sensing images of the study area. The relationships between landslides and various influencing factors have been explored through spatial analysis of geographic information science (GIS). Furthermore, comparisons have been made between the Gulang earthquake landslides and other seismic landslides in the Loess Plateau from macroscopic and microcosmic perspectives. A total of 807 earthquake landslides have been interpreted, with a total landslide area of 256.14 km2. The region with the seismic intensity of Ⅹ is the dominant area of landslide distribution. Also, most landslides are distributed at slope angles of (10° and 40°). The relative elevation difference of (100 m and 300 m) is the high-incidence range of landslides. Compared with seismic landslides in the Loess Plateau, the slope angle and the relative elevation difference of most landslides in the study area are larger. The strength of the loess structure in the study area is stronger than that in the Loess Plateau from the view of physical properties and microstructure.
Investigation of pollutant metals in road dust in a post-industrial city: Case study from Detroit, Michigan
Authors: Denny Max , Baskaran Mark , Burdick Scott , Tummala Chandra , Dittrich Timothy
Published: September 2022

Inhalation and/or ingestion of toxic heavy metals enriched in road dust have toxic effects on humans. From inhalation and ingestion of dust, release of readily bioavailable elements sorbed on to dust, could lead to health issues such as lung or skin cancer, kidney dysfunction, hypertension, dermal lesions, peripheral neuropathy, and vascular disease. On 49 road dust and soil samples collected in the Metro Detroit area, we analyzed a suite of trace metals in <63 μm and size-separated fractions to quantify the extent of metal enrichment and contamination status. We evaluated Enrichment Factor (EF), Pollution Index (PI), Geoaccumulation Index (GI), and Pollution Load Index (PLI). The average Chromium (Cr) concentration of 198 ppm is the highest reported for any global city in literature while in ∼80% samples, Barium (Ba), Cr, Lead (Pb), and Zinc (Zn) concentrations exceeded the USEPA limit. The EF for Barium (Ba) was found to be ≥9 in all samples, and for the rest of other elements was >1.5. The PI values for Cr, Ba, Zinc (Zn), Lead (Pb), and Selenium (Se) indicate elevated levels and for Ba, Cr, and Zn up to a moderate level. PLI values in >50% of road dusts were ≥3, indicating many sites are ‘heavily polluted’. The PLI value in Detroit road dust is higher than those reported for some other global cities including Shanghai. Observed strong correlations between Cr-Zn and Cadmium (Cd)-Silver (Ag) likely indicate a common metal source. Cluster analysis of data for Cd, Arsenic (As), Pb, and Zn appears to be distinct between different proximally related clusters, while Nickel (Ni) and Ba are potentially more distinct between property type clusters. In general, metal concentrations of industrial samples are more commonly distinct with over half of metals (≥6 of 11) differing from residential and/or soil samples. Three generalized “groups” were identified from the combination of hierarchical cluster and PCA analyses followed by varimax rotation: Group 1 characterized by Cr, Cd, Ni, Ag, Zn, and Copper (Cu); Group 2 characterized by Pb, As, Mercury (Hg), and minor Ba; and Group 3 characterized by Se. This is the first study to report all four metrics of metal pollution along with rigorous statistical analysis.
Seasonal differences in the dominant factors of surface urban heat islands along the urban-rural gradient
Authors: Xin Jiaxing , Yang Jun , Wang Ling-en , Jin Cui , Xiao Xiangming , Xia Jianhong (Cecilia)
Published: September 2022

Urbanization has been accelerating; hence the effect of urban heat island (UHI) has increased. There has been extensive research on spatiotemporal UHI changes and drivers, however, data on the dominant seasonal factors of UHIs and the differences along urban-rural gradients remain limited. Based on Luojia-1A, Landsat 8, and moderate resolution imaging spectroradiometer (MODIS) data, we assessed the seasonal differences in surface UHI (SUHI), normalized differences in vegetation index (NDVI), built-up index (NDBI), and water index (NDWI) and their relationships in the Dalian City, Northeast China. We found that in the urban built-up area, the mean SUHI intensity (SUHII) decreased from that in summer (2.74°C) > autumn (1.65°C) > winter (0.28°C) > spring (−0.79°C). SUHII was more strongly affected by NDWI and NDBI than NDVI, and NDBI and NDWI showed positive and negative correlations with SUHII in different seasons, while NDVI and SUHII were positively correlated in spring and negatively correlated in the other seasons. When analyzing the dominant factors of SUHII, the importance results showed that, in spring, NDBI > NDVI > NDWI, in autumn, NDVI > NDWI > NDBI, in summer and winter, NDWI > NDVI > NDBI. In addition, SUHII changed the most in summer along the urban-rural gradient, decreasing from 2.74°C to −2.74°C. Among these indicators, except for spring NDVI which increased from 0.09 to 0.59 with distance from built-up areas, there was minimal change in NDVI, NDBI, and NDWI along the urban-rural gradient in other seasons (i.e., all were within 0.2). In this study, the difference analysis of SUHI and remote sensing indices along the urban-rural gradient can help to facilitate the rational layout of cities.
Spatial–temporal distribution measurement of input–output efficiency of the water–energy–food nexus of the Yangtze River Economic Belt, China
Authors: Ni Yikun , Chen Yan
Published: September 2022

Water, energy, and food are important factors affecting people’s lives and socio-economic development, and their production and consumption processes are closely related, so it is necessary to do research on input–output efficiency as a whole. The research is conducive to promoting the effective utilization and rational allocation of relevant resources in the Yangtze River Economic Belt, thereby promoting sustainable development. This study calculates the input–output efficiency of the water–energy–food (WEF) nexus of 11 provincial administrative regions in the Yangtze River Economic Belt utilizing the DEA-BCC model. Then, new indicators called area expansion degree and the subsystem’s influence degree are proposed with the aid of the standard deviation ellipse model to analyze the characteristics and trends of spatial–temporal distribution of input–output efficiency. The standard deviation ellipse model starts from the basic spatial elements, including point, line, and surface, and is used to study the spatial distribution and trend change of efficiency according to the center of gravity and area. The shift of the center of gravity shows the direction of significant improvement in the effect of resource allocation, and the change of area shows the differences in the speed of efficiency improvement between regions and the future development trend. The results mainly indicate that the resource allocation in the middle and lower reaches of the Yangtze River Economic Belt is more reasonable than that in the upper reaches, and the efficiency distribution is obviously concentrated in the northeast direction. It is suggested that the provincial administrative regions in the upper reaches should optimize the industrial structure, the regions in the middle and lower reaches should improve the resource structure, and the flow of talents and technology of regions should be promoted.
Cytokinin biosynthesis in cyanobacteria: Insights for crop improvement
Authors: Uniyal Shashi , Bhandari Munni , Singh Preeti , Singh Rahul Kunwar , Tiwari Shree Prakash
Published: September 2022

Cytokinins, a type of phytohormones that induce division of cytoplasm, have considerable value in agriculture due to their influences on several physiological processes of plants such as morphogenesis, development of chloroplast, seed dormancy, leaf senescence, etc. Previously, it was assumed that plants obtain cytokinin from the soil produced by microbes as these hormones were first discovered in soil-inhabiting bacteria i.e., Agrobacterium tumefaciens. Later, the cytokinin biosynthesis gene, i.e., ipt gene, has been reported in plants too. Though plants synthesize cytokinins, several studies have reported that the exogenous application of cytokinins has numerous beneficial effects including the acceleration of plant growth and boosting economic yield. Cyanobacteria may be employed in the soil not only as the source of cytokinins but also as the source of other plant growth-promoting metabolites. These organisms biosynthesize the cytokinins using the enzyme isopentenyl transferases (IPTs) in a fashion similar to the plants; however, there are few differences in the biosynthesis mechanism of cytokinins in cyanobacteria and plants. Cytokinins are important for the establishment of interaction between plants and cyanobacteria as evidenced by gene knockout experiments. These hormones are also helpful in alleviating the adverse effects of abiotic stresses on plant development. Cyanobacterial supplements in the field result in the induction of adventitious roots and shoots on petiolar as well as internodal segments. The leaf, root, and stem explants of certain plants exhibited successful regeneration when treated with cyanobacterial extract/cell suspension. These successful regeneration practices mark the way of cyanobacterial deployment in the field as a great move toward the goal of sustainable agriculture.
Subtyping children with asthma by clustering analysis of mRNA expression data
Authors: Wang Ting , He Changhui , Hu Ming , Wu Honghua , Ou Shuteng , Li Yuke , Fan Chuping
Published: September 2022

Background: Asthma is a heterogeneous disease. There are several phenotypic classifications for childhood asthma.

Methods: Unsupervised consensus cluster analysis was used to classify 36 children with persistent asthma from the GSE65204 dataset. The differentially expressed genes (DEGs) between different asthma subtypes were identified, and weighted gene co-expression network analysis (WGCNA) was carried out. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed for DEGs and critical gene modules. Protein–protein interactions (PPI) were constructed to obtain the hub genes. Finally, differences in the immune microenvironment were analyzed between different subtypes.

Results: Two subtypes (C1, C2) were identified using unsupervised consensus clustering. The DEGs between different asthma subtypes were mainly enriched in immune regulation and the release of inflammatory mediators. The important modular genes screened by WGCNA were mainly enriched in aspects of inflammatory mediator regulation. PPI analysis found 10 hub genes (DRC1, TTC25, DNALI1, DNAI1, DNAI2, PIH1D3, ARMC4, RSPH1, DNAAF3, and DNAH5), and ROC analysis demonstrated that 10 hub genes had a reliably ability to distinguish C1 from C2. And we observed differences between C1 and C2 in their immune microenvironment.

Conclusion: Using the gene expression profiles of children’s nasal epithelium, we identified two asthma subtypes that have different gene expression patterns, biological characteristics, and immune microenvironments. This will provide a reference point for future childhood asthma typing and personalized therapy.
Cytokine competent gut-joint migratory T Cells contribute to inflammation in the joint
Authors: Lefferts Adam R. , Norman Eric , Claypool David J. , Kantheti Uma , Kuhn Kristine A.
Published: September 2022

Although studies have identified the presence of gut-associated cells in the enthesis of joints affected by spondylarthritis (SpA), a direct link through cellular transit between the gut and joint has yet to be formally demonstrated. Using KikGR transgenic mice to label in situ and track cellular trafficking from the distal colon to the joint under inflammatory conditions of both the gut and joint, we demonstrate bona-fide gut-joint trafficking of T cells from the colon epithelium, also called intraepithelial lymphocytes (IELs), to distal sites including joint enthesis, the pathogenic site of SpA. Similar to patients with SpA, colon IELs from the TNFΔARE/+ mouse model of inflammatory bowel disease and SpA display heightened TNF production upon stimulation. Using ex vivo stimulation of photo-labeled gut-joint trafficked T cells from the popliteal lymph nodes of KikGR and KikGR TNFΔARE/+ we saw that the CD4+ photo-labeled population was highly enriched for IL-17 competence in healthy as well as arthritic mice, however in the TNFΔARE/+ mice these cells were additionally enriched for TNF. Using transfer of magnetically isolated IELs from TNF+/+ and TNFΔARE/+ donors into Rag1-/- hosts, we confirmed that IELs can exacerbate inflammatory processes in the joint. Finally, we blocked IEL recruitment to the colon epithelium using broad spectrum antibiotics in TNFΔARE/+ mice. Antibiotic-treated mice had reduced gut-joint IEL migration, contained fewer Il-17A and TNF competent CD4+ T cells, and lessened joint pathology compared to untreated littermate controls. Together these results demonstrate that pro-inflammatory colon-derived IELs can exacerbate inflammatory responses in the joint through systemic trafficking, and that interference with this process through gut-targeted approaches has therapeutic potential in SpA.
The rabbit as an animal model to study innate immunity genes: Is it better than mice?
Authors: Soares João , Pinheiro Ana , Esteves Pedro José
Published: September 2022

The European rabbit (Oryctolagus cuniculus) was the first animal model used to understand human diseases like rabies and syphilis. Nowadays, the rabbit is still used to study several human infectious diseases like syphilis, HIV and papillomavirus. However, due to several mainly practical reasons, it has been replaced as an animal model by mice (Mus musculus). The rabbit and mouse share a recent common ancestor and are classified in the superorder Glires which arose at approximately 82 million years ago (mya). These species diverged from the Primates’ ancestor at around 92 million years ago and, as such, one expects the rabbit-human and mouse-human genetic distances to be very similar. To evaluate this hypothesis, we developed a set of tools for automatic data extraction, sequence alignment and similarity study, and a web application for visualization of the resulting data. We aligned and calculated the genetic distances for 2793 innate immune system genes from human, rabbit and mouse using sequences available in the NCBI database. The obtained results show that the rabbit-human genetic distance is lower than the mouse-human genetic distance for 88% of these genes. Furthermore, when we considered only genes with a difference in genetic distance higher than 0.05, this figure increase to 93%. These results can be explained by the increase of the mutation rates in the mouse lineage suggested by some authors and clearly show that, at least looking to the genetic distance to human genes, the European rabbit is a better model to study innate immune system genes than the mouse.
Bacterial outer membrane vesicles as a candidate tumor vaccine platform
Authors: Wang Shuming , Guo Jiayi , Bai Yang , Sun Cai , Wu Yanhao , Liu Zhe , Liu Xiaofei , Wang Yanfeng , Wang Zhigang , Zhang Yongmin , Hao Huifang
Published: September 2022

Cancer represents a serious concern for human life and health. Due to drug resistance and the easy metastasis of tumors, there is urgent need to develop new cancer treatment methods beyond the traditional radiotherapy, chemotherapy, and surgery. Bacterial outer membrane vesicles (OMVs) are a type of double-membrane vesicle secreted by Gram-negative bacteria in the process of growth and life, and play extremely important roles in the survival and invasion of those bacteria. In particular, OMVs contain a large number of immunogenic components associated with their parent bacterium, which can be used as vaccines, adjuvants, and vectors to treat diseases, especially in presenting tumor antigens or targeted therapy with small-molecule drugs. Some OMV-based vaccines are already on the market and have demonstrated good therapeutic effect on the corresponding diseases. OMV-based vaccines for cancer are also being studied, and some are already in clinical trials. This paper reviews bacterial outer membrane vesicles, their interaction with host cells, and their applications in tumor vaccines.
Determinants of prolonged hospitalization and mortality among leptospirosis patients attending tertiary care hospitals in northeastern state in peninsular Malaysia: A cross sectional retrospective analysis
Authors: Al Hariri Yassin K. , Sulaiman Syed A. S. , Khan Amer H. , Adnan Azreen S. , Al-Ebrahem Sundos Q.
Published: September 2022

Background

Leptospirosis is the most common anthropozoonosis worldwide and imposes a major public health problem in many tropical countries. It is a leading cause of disease burden in form of mortality, morbidity and hospital admission. Identifying patients at high risk for mortality or for prolonged hospitalization may save lives and preserve economy. The aim of the current study is to identify significant factors associated with disease mortality and prolonged hospitalization.

Design

Cress-sectional retrospective study.

Settings

Tertiary care teaching hospitals in Kelantan, Peninsular Malaysia.

Participants

Adult patients proven to have leptospirosis depending on IgM ELISA were classified into two classes depending on prolonged hospitalization (>7 days or ≤ 7 days) and mortality (fatal cases or non-fatal cases). Patients' clinico-laboratory data were compared according to these two outcomes using the appropriate statistical test.

Results

Of the 525 patients enrolled, 136 (25.9%) had prolonged hospitalization. The mean length of stay was 6.77 ± 5.68 days. Logistic regression analysis identified acute kidney injury (AKI) (OR 2.3), Jaundice (OR 2.7), elevated alanine aminotransferase (ALT) (OR 2), and prolonged prothrombin time (PT) (OR 1.9) independently associated with prolonged hospitalization. Case fatality rate was 6.48% and around one third of fatal cases had prolonged hospitalization of more than seven days. Factors associated with leptospirosis mortality included age >40 years (p < 0.001), patients presented with tachypnea (p = 0.002), pulmonary infiltrate (p < 0.001), T-wave changes (p < 0.001), atrial fibrillation (p = 0.013), conducting abnormality (p < 0.001), chronic kidney diseases (p < 0.001), multiple organ dysfunctions (p < 0.0010), respiratory failure (p < 0.001), pneumonia (p < 0.001), sepsis (p = 0.004), low venous PH (p = 0.042), AKI (P < 0.001), elevated AST (p < 0.001) or ALT (p = 0.004), hypoalbuminemia (p < 0.001), rhabdomyolysis (p < 0.001), severe thrombocytopenia (p = 0.042), prolonged PT (p < 0.001) or prolonged aPTT (p < 0.017).

Conclusions

Significant proportion of leptospirosis patients (25.9%) had prolonged hospital stay and less proportion died (6.48%). Early identifying patients with factors associated with prolonged hospitalization and death will positively impact practitioners' decisions regarding the proper and fast course of management including ICU admission.
Changes in the genomes and methylomes of three Salmonella enterica serovars after long-term storage in ground black pepper
Authors: Davies Cary P. , Jurkiw Thomas , Haendiges Julie , Reed Elizabeth , Anderson Nathan , Grasso-Kelley Elizabeth , Hoffmann Maria , Zheng Jie
Published: September 2022

Low moisture foods (LMFs) have traditionally been recognized as safe for consumption, as most bacteria require higher water content to grow. However, outbreaks due to LMF foods are increasing, and the microbial pathogen Salmonella enterica is frequently implicated. S. enterica can survive in LMFs for years, but few serovars have been studied, and the mechanisms which underlie this longevity are not well understood. Here, we determine that S. enterica serovars S. Tennessee, S. Anatum, and S. Reading but not S. Oranienburg can survive in the ground black pepper for 6 years. S. Reading was not previously associated with any LMF. Using both Illumina and Pacific Biosciences sequencing technologies, we also document changes in the genomes and methylomes of the surviving serovars over this 6-year period. The three serovars acquired a small number of single nucleotide polymorphisms (SNPs) including seven substitutions (four synonymous, two non-synonymous, and one substitution in a non-coding region), and two insertion-deletions. Nine distinct N6-methyladenine (m6A) methylated motifs across the three serovars were identified including five which were previously known, Gm6ATC, CAGm6AG, BATGCm6AT, CRTm6AYN6CTC, and CCm6AN7TGAG, and four novel serovar-specific motifs, GRTm6AN8TTYG, GAm6ACN7GTA, GAA m6ACY, and CAAm6ANCC. Interestingly, the BATGCAT motif was incompletely methylated (35–64% sites across the genome methylated), suggesting a possible role in gene regulation. Furthermore, the number of methylated BATGCm6AT motifs increased after storage in ground black pepper for 6 years from 475 to 657 (S. Tennessee), 366 to 608 (S. Anatum), and 525 to 570 (S. Reading), thus warranting further study as an adaptive mechanism. This is the first long-term assessment of genomic changes in S. enterica in a low moisture environment, and the first study to examine the methylome of any bacteria over a period of years, to our knowledge. These data contribute to our understanding of S. enterica survival in LMFs, and coupled with further studies, will provide the information necessary to design effective interventions which reduce S. enterica in LMFs and maintain a healthy, safe food supply.
Comorbidity in patients with first-ever ischemic stroke: Disease patterns and their associations with cognitive and physical function
Authors: She Rui , Yan Zhongrui , Hao Yanlei , Zhang Zuoji , Du Yifeng , Liang Yajun , Vetrano Davide L. , Dekker Joost , Bai Bo , Lau Joseph T. F. , Qiu Chengxuan
Published: September 2022

The present study examined the prevalence and pattern of comorbidity among Chinese patients with first-ever acute ischemic stroke, and assessed the associations of specific comorbidity patterns with physical and cognitive functioning after stroke occurrence. A hospital-based cross-sectional study was conducted among 2,151 patients with first-ever ischemic stroke (age ≥40 years; 64.2% men) who were admitted to two university hospitals in Shandong, China between 2016 and 2017. Data on demographics, lifestyles, chronic health conditions, and use of medications were collected through in-person interviews, clinical examinations, and laboratory tests. Physical functioning was assessed by the Barthel index (BI) and the modified Rankin Scale (mRS) while cognitive functioning was assessed by the Montreal Cognitive Assessment test. The results showed that comorbidity was present in 90.9% of the stroke patients (women vs. men: 95.2 vs. 88.7%, P < 0.001). Exploratory factor analysis identified three patterns of comorbidity, i.e., patterns of degenerative-cardiopulmonary, heart-gastrointestinal-psychiatric, and metabolic-kidney diseases. The number of comorbidities was significantly associated with a higher likelihood of moderate-to-severe physical dependence [odds ratio (95% CI) = 1.15 (1.06–1.25) for BI and 1.12 (1.04–1.21) for mRS, all P < 0.01] and cognitive impairment [odds ratio (95% CI) = 1.11 (1.02–1.20), P = 0.017], after adjusting for multiple covariates. Almost all the three comorbidity patterns were associated with increased likelihoods of physical dependence (range for odds ratios: 1.26–1.33) and cognitive impairment (range for odds ratios: 1.25–1.34). No significant association was found between degenerative-cardiopulmonary pattern and mRS. These findings suggest that comorbidity is associated with poor physical and cognitive functioning during the acute phase of ischemic stroke. Routine assessments of comorbidity and cognitive and physical function among patients with acute ischemic stroke should be considered in stroke research and clinical practice.
Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer’s disease model mice independent of brain amyloid β pathology
Authors: Kasica Nicole P. , Zhou Xueyan , Jester Hannah M. , Holland Caroline E. , Ryazanov Alexey G. , Forshaw Tom E. , Furdui Cristina M. , Ma Tao
Published: September 2022

Maintenance of memory and synaptic plasticity depends on de novo protein synthesis, and accumulating evidence implicates a role of dysregulated mRNA translation in cognitive impairments associated with Alzheimer’s disease (AD). Accumulating evidence demonstrates hyper-phosphorylation of translation factor eukaryotic elongation factor 2 (eEF2) in the hippocampi of human AD patients as well as transgenic AD model mice. Phosphorylation of eEF2 (at the Thr 56 site) by its only known kinase, eEF2K, leads to inhibition of general protein synthesis. A recent study suggests that amyloid β (Aβ)-induced neurotoxicity could be associated with an interaction between eEF2 phosphorylation and the transcription factor nuclear erythroid 2-related factor (NRF2)-mediated antioxidant response. In this brief communication, we report that global homozygous knockout of the eEF2K gene alleviates deficits of long-term recognition and spatial learning in a mouse model of AD (APP/PS1). Moreover, eEF2K knockout does not alter brain Aβ pathology in APP/PS1 mice. The hippocampal NRF2 antioxidant response in the APP/PS1 mice, measured by expression levels of nicotinamide adenine dinucleotide plus hydrogen (NADPH) quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1), is ameliorated by suppression of eEF2K signaling. Together, the findings may contribute to our understanding of the molecular mechanisms underlying AD pathogenesis, indicating that suppression of eEF2K activity could be a beneficial therapeutic option for this devastating neurodegenerative disease.
CAMTA1 gene affects the ischemia-reperfusion injury by regulating CCND1
Authors: Liu Yang , Shang Guohui , Zhang Xuran , Liu Fuyong , Zhang Chi , Li Zhihao , Jia Jing , Xu Yan , Zhang Zhaojing , Yang Shangdong , Zhou Baixue , Luan Yingying , Huang Yanyang , Peng Yue , Han Tianyi , He Ying , Zheng Hong
Published: September 2022

Epigenetic modulations lead to changes in gene expression, including DNA methylation, histone modifications, and noncoding RNAs. In recent years, epigenetic modifications have been related to the pathogenesis of different types of cancer, cardiovascular disease, and other diseases. Emerging evidence indicates that DNA methylation could be associated with ischemic stroke (IS) and plays a role in pathological progression, but the underlying mechanism has not yet been fully understood. In this study, we used human methylation 850K BeadChip to analyze the differences in gene methylation status in the peripheral blood samples from two groups (3 IS patients vs. 3 healthy controls). According to their bioinformatics profiling, we found 278 genes with significantly different methylation levels. Seven genes with the most significant methylation modifications were validated in two expanded groups (100 IS patients vs. 100 healthy controls). The CAMTA1 gene had significantly different methylation changes in patients compared to the controls. To understand the CAMTA1 function in stroke, we generated CAMTA1 knockout in SH-SY5Y cells. RNA seq results in CAMTA1 knockout cells revealed the pathways and gene set enrichments involved in cellular proliferation and cell cycle. Furthermore, a series of experiments demonstrated that in the oxygen-glucose deprivation/re-oxygenation (OGD/R) model system, the expression of cyclin D1, an essential regulator of cell cycle progression, was increased in SH-SY5Y CAMTA1 KO cells. Increasing evidence demonstrated that ischemic stress could inappropriately raise cyclin D1 levels in mature neurons. However, the molecular signals leading to an increased cyclin D1 level are unclear. Our findings demonstrate for the first time that the CAMTA1 gene could regulate cyclin D1 expression and implicate their role in strokes.
Auditory clicks elicit equivalent temporal frequency perception to tactile pulses: A cross-modal psychophysical study
Authors: Sharma Deepak , Ng Kevin K. W. , Birznieks Ingvars , Vickery Richard M.
Published: September 2022

Both hearing and touch are sensitive to the frequency of mechanical oscillations—sound waves and tactile vibrations, respectively. The mounting evidence of parallels in temporal frequency processing between the two sensory systems led us to directly address the question of perceptual frequency equivalence between touch and hearing using stimuli of simple and more complex temporal features. In a cross-modal psychophysical paradigm, subjects compared the perceived frequency of pulsatile mechanical vibrations to that elicited by pulsatile acoustic (click) trains, and vice versa. Non-invasive pulsatile stimulation designed to excite a fixed population of afferents was used to induce desired temporal spike trains at frequencies spanning flutter up to vibratory hum (>50 Hz). The cross-modal perceived frequency for regular test pulse trains of either modality was a close match to the presented stimulus physical frequency up to 100 Hz. We then tested whether the recently discovered “burst gap” temporal code for frequency, that is shared by the two senses, renders an equivalent cross-modal frequency perception. When subjects compared trains comprising pairs of pulses (bursts) in one modality against regular trains in the other, the cross-sensory equivalent perceptual frequency best corresponded to the silent interval between the successive bursts in both auditory and tactile test stimuli. These findings suggest that identical acoustic and vibrotactile pulse trains, regardless of pattern, elicit equivalent frequencies, and imply analogous temporal frequency computation strategies in both modalities. This perceptual correspondence raises the possibility of employing a cross-modal comparison as a robust standard to overcome the prevailing methodological limitations in psychophysical investigations and strongly encourages cross-modal approaches for transmitting sensory information such as translating pitch into a similar pattern of vibration on the skin.
Visualizing stellate ganglion with US imaging for guided SGB treatment: A feasibility study with healthy adults
Authors: Li Jia , Pu Shaofeng , Liu Zihao , Jiang Lixin , Zheng Yuanyi
Published: September 2022

Objective

As for ultrasound (US) guided stellate ganglion (SG) block, unsatisfactory curative outcomes and complications still remain. This problem could be greatly improved by identifying and monitoring SG. To the best of our knowledge, there are few reports to directly visualize SG in literature. This study explored the feasibility of detection of SG and summarized the findings of SG through US.

Methods

Fifty healthy adults with 100 SGs were enrolled. The size, shape, echogenicity, margin, the inferior pole of SG, the relationship between the superior pole of SG and the transverse process, the relationship between the superior pole of SG and the inferior thyroid artery, and the relationships between SG and other surrounding tissues were evaluated by US.

Results

The SG was identified in 79% of the participants. No significant differences were found between the right and left sides regarding thickness, cross-sectional area (CSA), and position (all p > 0.05); however, there was a significant difference in the width of the right and left sides (p < 0.05). Side was associated with SG visibility (p < 0.05), however, the gender was not (p > 0.05). A total of 42% of SGs were oval-shaped. All SGs were hyperechogenic and had an ill-defined margin. In fact, 63% of SGs were located in the C7 transverse process level, 77% of SGs were located under the inferior thyroid artery, and all of these SGs were located lateral to the thyroid and medial to the anterior scalene muscle and the vagus nerve.

Conclusion

Our preliminary study demonstrates that US imaging provides the capability of detecting SG. This may be helpful in minimizing complications and improving the accuracy of US-guided SG block.
Modality specific roles for metabotropic GABAergic signaling and calcium induced calcium release mechanisms in regulating cold nociception
Authors: Patel Atit A. , Sakurai Akira , Himmel Nathaniel J. , Cox Daniel N.
Published: September 2022

Calcium (Ca2+) plays a pivotal role in modulating neuronal-mediated responses to modality-specific sensory stimuli. Recent studies in Drosophila reveal class III (CIII) multidendritic (md) sensory neurons function as multimodal sensors regulating distinct behavioral responses to innocuous mechanical and nociceptive thermal stimuli. Functional analyses revealed CIII-mediated multimodal behavioral output is dependent upon activation levels with stimulus-evoked Ca2+ displaying relatively low vs. high intracellular levels in response to gentle touch vs. noxious cold, respectively. However, the mechanistic bases underlying modality-specific differential Ca2+ responses in CIII neurons remain incompletely understood. We hypothesized that noxious cold-evoked high intracellular Ca2+ responses in CIII neurons may rely upon Ca2+ induced Ca2+ release (CICR) mechanisms involving transient receptor potential (TRP) channels and/or metabotropic G protein coupled receptor (GPCR) activation to promote cold nociceptive behaviors. Mutant and/or CIII-specific knockdown of GPCR and CICR signaling molecules [GABAB-R2, Gαq, phospholipase C, ryanodine receptor (RyR) and Inositol trisphosphate receptor (IP3R)] led to impaired cold-evoked nociceptive behavior. GPCR mediated signaling, through GABAB-R2 and IP3R, is not required in CIII neurons for innocuous touch evoked behaviors. However, CICR via RyR is required for innocuous touch-evoked behaviors. Disruptions in GABAB-R2, IP3R, and RyR in CIII neurons leads to significantly lower levels of cold-evoked Ca2+ responses indicating GPCR and CICR signaling mechanisms function in regulating Ca2+ release. CIII neurons exhibit bipartite cold-evoked firing patterns, where CIII neurons burst during rapid temperature change and tonically fire during steady state cold temperatures. GABAB-R2 knockdown in CIII neurons resulted in disorganized firing patterns during cold exposure. We further demonstrate that application of GABA or the GABAB specific agonist baclofen potentiates cold-evoked CIII neuron activity. Upon ryanodine application, CIII neurons exhibit increased bursting activity and with CIII-specific RyR knockdown, there is an increase in cold-evoked tonic firing and decrease in bursting. Lastly, our previous studies implicated the TRPP channel Pkd2 in cold nociception, and here, we show that Pkd2 and IP3R genetically interact to specifically regulate cold-evoked behavior, but not innocuous mechanosensation. Collectively, these analyses support novel, modality-specific roles for metabotropic GABAergic signaling and CICR mechanisms in regulating intracellular Ca2+ levels and cold-evoked behavioral output from multimodal CIII neurons.
A comparative study of nutrient composition, bioactive properties and phytochemical characteristics of Stauntonia obovatifoliola flesh and pericarp
Authors: Li Rurui , Ru Yuerong , Feng Ling , Wang Zhenxing , He Xiahong , Zhang Xuechun
Published: September 2022

A comparative study was conducted among the flesh (SOF) and pericarp (SOP) of Stauntonia obovatifoliola, a wild edible fruit in China. The nutrient composition of both these tissues was firstly quantified, and liquid-liquid extraction was then used to separate their methanolic extracts to get petroleum ether, chloroform, ethyl acetate, n-butanol, and residual aqueous fractions, which were evaluated for their total phenol content (TPC), total flavonoid content (TFC), antioxidant capacities, and α-glucosidase and acetylcholinesterase inhibition abilities. Finally, high-performance liquid chromatography (HPLC) was used to analyze their phytochemical composition. The results revealed the excellent nutritional properties of both SOF and SOP, especially SOP (total dietary fiber, 15.50 g/100 g; total amino acids, 0.80 g/100 g; vitamin C, 18.00 mg/100 g; Ca, 272.00 mg/kg; K, 402.00 mg/100 g). For both tissues, their ethyl acetate fractions showed the highest TPC (355.12 and 390.99 mg GAE/g DE) and TFC (306.58 and 298.48 mg RE/g DE). Surprisingly, the ethyl acetate fraction of SOP exhibited the strongest DPPH and ABTS radical scavenging capacity with 1046.94 and 1298.64 mg Trolox/g, respectively, which were higher than that of controls Vc and BHT. In contrast, their chloroform fractions exhibited the strongest ferric reducing antioxidant power (1903.05 and 1407.11 mg FeSO4/g DE) and oxygen radical absorbance capacity (951.12 and 1510.21 mg Trolox/g DE). In addition, the ethyl acetate fraction of SOF displayed superior α-glucosidase inhibition ability with the IC50 value of 0.19 mg/mL, which was comparable to control acarbose. In comparison, the ethyl acetate fraction of SOP had the best acetylcholinesterase inhibition ability with the IC50 value of 0.47 mg/mL. The HPLC analysis results demonstrated that the ethyl acetate fraction of SOP showed significantly higher phenolic content, particularly for phenolic acids (p-hydroxybenzoic acid, 8.00 ± 0.65 mg/g) and flavonoids (epicatechin, 28.63 ± 1.26 mg/g), as compared to other samples. The above results suggest that Stauntonia obovatifoliola, especially its pericarp, had excellent nutrient compositions, bioactive properties and phytochemical characteristics, and had the potential to be developed as natural functional food.
Nutritional risk screening 2002 scale and subsequent risk of stroke-associated infection in ischemic stroke: The REMISE study
Authors: Chen Xiaoli , Li Dongze , Liu Yi , Zhu Ling , Jia Yu , Gao Yongli
Published: September 2022

Background and aim

Stroke-associated infection (SAI) is a common and serious complication in patients with IS. This study aimed to evaluate the impact of nutritional status at admission assessed on SAI, explore the predictive value of the Nutritional Risk Screening 2002 (NRS-2002 for SAI.

Methods

This study included patients with IS who were admitted to five major hospitals in Chengdu from January 2017 to February 2019. The nutritional status was assessed using the NRS-2002 tool. Logistic regression analysis was performed to explore the predictive value of NRS-2002 for SAI.

Results

A total of 594 patients with IS were included in this study; among them, 215 (36.20%) patients were at risk of malnutrition, and 216 (36.36%) patients developed SAI. The area under the curve of the NRS-2002 scores was smaller than A2DS2 (0.644 vs. 0.779), and NRS-2002 improved the predictive values of the A2DS2 score(Age, Atrial fibrillation, Dysphagia, Sex, Stroke Severity) for SAI (P < 0.001). Logistic regression analysis showed that patients with NRS-2002 score ≥ 3 had significantly higher risks of SAI (NRS-2002: odds ratios (OR) = 1.450, 95% confidence interval (CI): 1.184–1.692, P < 0.001).

Conclusion

NRS-2002 is a useful and simple tool for identifying the risk of SAI. Malnutrition is related to the development of SAI. Malnourished patients with stroke may benefit from further nutritional supplements and management.