bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Harga dan Promosi terhadap Keputusan Pembelian pada Story-I Mall Alam Sutera
Authors: Noviyanti Iis , Hayati Feb Amni , Saputra Rizki
Published: December 2019

Abstrak
Tujuan penelitian ini adalah untuk mengetahui pengaruh harga dan promosi terhadap keputusan penjualan pada Story-I Mall Alam Sutera. Pendekatan penelitian yang digunakan dalam penyusunan penelitian ini adalah kuantitatif. Adapun populasi dalam penelitian ini adalah pelanggan pada Story-I Mall Alam Sutera, sedangkan teknik sampel yang digunakan adalah dengan rumus Rao Purba dan jumlah Sampel 100 responden yang mewakili populasi. Metode pengumpulan data metode likert dan interval. Metode yang digunakan untuk menguji dan menganalisis data adalah uji validitas, uji reliabilitas, koefisien korelasi, uji heteroskedastisitas, regresi linier berganda, uji T, uji F, koefisien determinasi, dan uji hipotesis. Hasil analisis data menunjukan harga dan promosi pada Story-I Mall Alam Sutera berpengaruh terhadap keputusan pembelian, hal ini dapat ditunjukan oleh nilai persamaan/model regresi Y = -6.587 + 0,386 X1 + 0,873 X2 dengan koefisien regresi X1 sebesar 0,368, X2 sebesar 0,873 dan nilai thitung lebih besar dari ttabel untuk kedua variabel bebas yaitu Harga X1 (2,745>1,985) Promosi X2 (6,938>1,985). Kontribusi pengaruh harga dan promosi terhadap keputusan pembelian 71,5 % sedangkan sisanya sebesar 28,5% dipengaruhi oleh faktor lain yang tidak diteliti.
AbstractThe purpose of this study was to study the interaction of prices and promotions on sales decisions at Story-I Mall Alam Sutera. This research used in this research is quantitative. The mall in this study is a customer at Alam Sutera Mall Story-I, while the sampling technique used is the Rao Purba formula and the number of samples is 100 respondents representing the population. Data collection methods Likert and interval methods. The method used to test and analyze data is the validity test, reliability test, trial, heteroscedasticity test, multiple linear regression, T-test, F test, coefficient of determination, and hypothesis testing. The results of data analysis show that prices and promotions in Story-I Mall Alam Sutera affect purchasing decisions, this can be shown by the value of the equation/regression model Y = -6.587 + 0.386 X1 + 0.873 X2 with a regression coefficient of 0.368, X2 of 0.873 and t-value is greater than t-table for the two independent variables, namely Price X1 (2.745> 1.985) X2 Promotion (6.938> 1.985). The contribution of the price and promotion to the purchase decision was 71.5% while the remaining 28.5% was approved by other factors that were not approved.
Niat Keluarga Terhadap Peningkatan Peran Mencegah Sedentary Lifestyle Remaja
Authors: Sholihah Mar'atus , Soedirham Oedojo , Triharini Mira
Published: December 2019

Introduction: Sedentary lifestyle cause obesity, hypertension, myopia, anxiety, and decreased adolescent academic abilities. The family's role prevent sedentary lifestyles is not optimal, even though the sedentary lifestyle of adolescent at home is difficult to control because they do not have written regulations on how to watch TV, use mobile phones, computers, and electronic games. The purpose of this study was to explore intention of family to increase role prevent sedentary lifestyle in adolescent. Methods: This research was descriptive study, with sample of 122 mothers of 10 Sugio Muhammadiyah High School students in Lamongan Regency in January 2019, selected using stratified random sampling. Data were collected using a questionnaire and analyzed using descriptive. Results: The results of this study was majority intention of family on suficient category had family role on suficient category too. Conclusion: Intention of family unsuficient will be have family role also unsuficient. Nurse can give health prmotion about sedentary lifestyle and increasing family intention to improve roles by applying GERMAS (Healthy Community Movement)

Keywords: intention; family; role; sedentary lifestyle; adolescent

ABSTRAK

Pendahuluan: sedentary lifestyle pada remaja dapat menyebabkan obesitas, hipertensi, myopi, kecemasan, dan penurunan kemampuan akademik. Peran keluarga mencegah sedentary lifestyle kurang optimal, padahal sedentary lifestyle remaja saat di rumah sulit dikendalikan karena tidak memiliki peraturan tertulis mengenai cara menonton TV, menggunakan HP, komputer, dan electronic game. Tujuan penelitian ini untuk melihat niat keluarga terhadap peningkatan peran mencegah sedentary lifestyle remaja. Metode: penelitian ini merupakan penelitian deskriptif dengan sampel 122 responden dengan teknik stratified random sampling pada keluarga siswa SMA Muhammadiayah 10 Sugio Kabupaten Lamongan, Jawa Timur. Data dikumpulkan melalui pengisian kuesioner lalu dianalisis secara deskriptif. Hasil: Hasil penelitian menunjukan niat keluarga sebagian besar memiliki dalam kategori cukup dengan peran keluarga mencegah sedentary lifestyle remaja yang juga dalam kategori cukup. Kesimpulan: Niat keluarga yang kurang untuk meningkatkan peran membuat peran keluarga yang dilakukan juga kurang sehingga meningkatkan resiko terjadi dampak sedentary lifestyle pada remaja. Perawat diharapkan memberikan promosi kesehatan tentang pengertian, bentuk, penyebab dan dampak sedentary lifestyle pada remaja, sehingga meningkatkan niat keluarga dahulu untuk merubah peran, kemudian memberikan intervensi peningkatan peran keluarga dengan mengajarkan cara penerapan GERMAS (Gerakan Masyarakat Sehat).

Kata kunci: niat; keluarga; peran; sedentary lifestyle; remaja
Pencegahan Tuberkulosis Paru dalam Keluarga: Kajian Literatur
Authors: Paneo Sri Ayu Rahayu S. , Nursasi Astuti Yuni
Published: December 2019

Tuberculosis is an infectious disease caused by Mycobaterium Tuberculosis and transmitted through sputum sprinkling. Tuberculosis disease have increase every year which is a contributor of mortality in the world. Transmission of tuberculosis is growing and resulting in increased transmission in the family and community environment. Families who are in contact with tuberculosis sufferers being a vulnerable populations to be infected. Various types of efforts are needed to prevent tuberculosis transmission. This article aims to explore the prevention of tuberculosis in the family. The literature review came from articles obtained from Pubmed, ScienceDirect, Wiley, SAGE Publications, Google Scholar and EBSCOHost since 2010-2019. The results of the study found that the prevention of transmission infection in the family were; provide immunization to babies, fulfill adequate nutrition for sufferers and family members, modify the home environment and control tuberculosis patients to get regular treatment. The role of the family becomes very important in prevention of transmission and the role of professional community nurses is also needed in order to strengthen aspects of health care services for tuberculosis sufferers. Family and nurse collaboration is a matter that needs to be increased simultaneously for optimal community health..

Keywords: prevention of transmission; nursing; family roles; tuberculosis treatment

ABSTRAK

Penyakit tuberkulosis adalah penyakit menular yang disebabkan oleh bakteri Mycobaterium Tuberculosis dan ditularkan melalui percikan dahak. Penyakit ini terus mengalami peningkatan di tiap tahunnya dimana merupakan penyumbang kematian di dunia. Penularan penyakit ini semakin berkembang dan mengakibatkan pada peningkatan penularan di lingkungan keluarga dan masyarakat. Keluarga yang sekontak dengan penderita tuberkulosis menjadi populasi yang paling rentan terinfeksi sehingga dibutuhkan berbagai macam upaya pencegahan penularan tuberkulosis. Artikel ini bertujuan untuk menggali bentuk pencegahan tuberkulosis dalam keluarga. Telaah literatur berasal dari artikel yang didapatkan dari Pubmed, ScienceDirect, Wiley, SAGE Publications, Google Scholar dan EBSCOHost sejak tahun 2010-2019. Hasil kajian yang didapatkan ditemukan bahwa bentuk pencegahan yang dapat dilakukan dalam memutus rantai penularan infeksi dalam keluarga yang terdiri dari; memberikan imunisasi pada bayi, pemenuhan nutrisi yang adekuat bagi penderita maupun anggota keluarga, modifikasi lingkungan rumah serta kontrol penderita tuberkulosis agar berobat dengan teratur. Peran keluarga menjadi sangat penting dalam upaya memutus rantai penularan dan peran perawat komunitas yang professional juga dibutuhkan dalam rangka menguatkan aspek layanan perawatan kesehatan untuk penderita tuberkulosis. Kerjasama keluarga dan perawat menjadi hal yang perlu ditingkatkan secara simultan demi kesehatan masyarakat yang optimal.

Kata kunci: pencegahan penularan; keperawatan; peran keluarga; pengobatan tuberkulosis
The Relationship Between Primary Health Care Services and Pregnancy Diagnosis and Follow-Up
Authors: Kurt Abdullah Kaan , Set Turan , Ates Elif
Published: December 2019

Aim: The aim of this study was to determine the effectiveness of family medicine in the detection of pregnancies, the status of pregnant women being registered to family medicine, the time of first contact with the family physician, and the rate of monitoring by family physicians.

Methods: This cross-sectional descriptive study was carried out at the pregnant follow-up polyclinic of a university hospital. The pilot study of the study was conducted with 15 participants. Data of 139 participants were analyzed.

Results: The median of pregnancy detection week was 5.00 (IQR:4.00-6.00). For the first blood test 23.7% (n=33) of the pregnant women had applied to the family physicians. It was determined that all pregnant women received prenatal care from healthcare workers within the first 14 weeks. The rate of pregnant women who received prenatal care from family medicine at any time of pregnancy was 89.9% (n=125).

Conclusion: It is seen that family medicine is not used effectively enough for pregnancy detection. The fact that knowing family medicine by almost all of the individuals, and informing the community will increase the rate and quality of application to family medicine. Improvement of family medicine pregnancy diagnosis and follow-up rates can be ensured by giving the necessary importance to the 15-49 age group follow-up and with the applicability of the referral chain.

Keywords: primary care, family practice, pregnancy, pregnancy test
Análisis de la inversión pública en educación, saneamiento y transporte en el distrito de Sanagorán, La Libertad- Perú
Authors: Munsibay Muñoa Manuel Alberto , Cavero-Egúsquiza Vargas Lauralinda Leonor , Carhuancho Mendoza Irma Milagros
Published: December 2019

La investigación tiene como objetivo analizar la inversión pública en los sectores de educación, saneamiento y transporte en Sanagorán, La Libertad - Perú. La metodología empleada para esta investigación fue de enfoque cualitativo y estudio de caso, se aplicó la técnica de la entrevista y el análisis documental. Para el análisis se reunió información de los proyectos de inversión pública y de los indicadores de cada sector en estudio, utilizando la base de datos del Banco Central de Reserva del Perú y del Ministerio de Economía y Finanzas. La investigación concluyó que más del 75% de proyectos de inversión pública se encuentran en situación de viable. En el sector educación las obras lograron beneficiar al 97.85% de la zona rural, significando el 21.24% del presupuesto para el año 2018, mientras que en saneamiento se invirtió el 24.99% enfatizando en la ampliación del sistema de agua potable por no contar con dicho servicio básico, finalmente en transporte se invirtió el 27.33% para la construcción de trochas carrózales.
Comunicación 2.0 en Ecuador. Análisis de las principales empresas del sector industrial
Authors: Valdiviezo Abad Karen Cesibel , Abendaño Mónica
Published: December 2019

La comunicación en las diferentes organizaciones evoluciona permanentemente y en las empresas del sector industrial del Ecuador en los últimos 10 años ha logrado un gran crecimiento, de manera especial en el entorno digital. Las organizaciones se ven en la necesidad de comunicar su accionar del día a día, pero actualmente son los públicos quienes demandan de información transparente, real y oportuna de las diferentes actividades que realizan las organizaciones y se lo requiere en diferentes formatos y plataformas. El presente estudio se profundiza y analiza la comunicación digital 2.0 de las 180 principales empresas del sector industrial de Ecuador. Se considera como fuente de información, el ranking de la revista Ekos del año 2017. En la investigación, se analiza los sitios web y medios sociales, tanto Facebook como Instagram, así como también espacios de primer contacto con los públicos, durante el periodo de enero a junio de 2018. Se identificaron diversas variables de investigación para el caso de los sitios web y las redes sociales. Los resultados que se presentan demuestran como las empresas manejan la comunicación digital de cara a sus públicos y la aprobación que logran por la gestión digital. Como otro resultado se evidencia el uso de la tecnología y la innovación en el campo de la comunicación por parte de las empresas caso de estudio.
Robust Speech Hashing for Digital Audio Forensics
Authors: Renza Diego , Vargas Jaisson , Ballesteros Dora M.
Published: December 2019

The verification of the integrity and authenticity of multimedia content is an essential task in the forensic field, in order to make digital evidence admissible. The main objective is to establish whether the multimedia content has been manipulated with significant changes to its content, such as the removal of noise (e.g., a gunshot) that could clarify the facts of a crime. In this project we propose a method to generate a summary value for audio recordings, known as hash. Our method is robust, which means that if the audio has been modified slightly (without changing its significant content) with perceptual manipulations such as MPEG-4 AAC, the hash value of the new audio is very similar to that of the original audio; on the contrary, if the audio is altered and its content changes, for example with a low pass filter, the new hash value moves away from the original value. The method starts with the application of MFCC (Mel-frequency cepstrum coefficients) and the reduction of dimensions through the analysis of main components (principal component analysis, PCA). The reduced data is encrypted using as inputs two values from a particular binarization system using Collatz conjecture as the basis. Finally, a robust 96-bit code is obtained, which varies little when perceptual modifications are made to the signal such as compression or amplitude modification. According to experimental tests, the BER (bit error rate) between the hash value of the original audio recording and the manipulated audio recording is low for perceptual manipulations, i.e., 0% for FLAC and re-quantization, 1% in average for volume (−6 dB gain), less than 5% in average for MPEG-4 and resampling (using the FIR anti-aliasing filter); but more than 25% for non-perceptual manipulations such as low pass filtering (3 kHz, fifth order), additive noise, cutting and copy-move.
Development of Deep Learning Based Human-Centered Threat Assessment for Application to Automated Driving Vehicle
Authors: Shin Donghoon , Kim Hyun-geun , Park Kang-moon , Yi Kyongsu
Published: December 2019

This paper describes the development of deep learning based human-centered threat assessment for application to automated driving vehicle. To achieve naturalistic driver model that would feel natural while safe to a human driver, manual driving characteristics are investigated through real-world driving test data. A probabilistic threat assessment with predicted collision time and collision probability is conducted to evaluate driving situations. On the basis of collision risk analysis, two kinds of deep learning have been implemented to reflect human driving characteristics for automated driving. A deep neural network (DNN) and recurrent neural network (RNN) are designed by neural architecture search (NAS), and by learning from the sequential data, respectively. The NAS is used to automatically design the individual driver’s neural network for efficient and effortless design process while ensuring training performance. Sequential trends in the host vehicle’s state can be incorporated through hand-made RNN. It has been shown from human-centered risk assessment simulations that two successfully designed deep learning driver models can provide conservative and progressive driving behavior similar to a manual human driver in both acceleration and deceleration situations by preventing collision.
Introducing Technical Indicators to Electricity Price Forecasting: A Feature Engineering Study for Linear, Ensemble, and Deep Machine Learning Models
Authors: Demir Sumeyra , Mincev Krystof , Kok Koen , Paterakis Nikolaos G.
Published: December 2019

Day-ahead electricity market (DAM) volatility and price forecast errors have grown in recent years. Changing market conditions, epitomised by increasing renewable energy production and rising intraday market trading, have spurred this growth. If forecast accuracies of DAM prices are to improve, new features capable of capturing the effects of technical or fundamental price drivers must be identified. In this paper, we focus on identifying/engineering technical features capable of capturing the behavioural biases of DAM traders. Technical indicators (TIs), such as Bollinger Bands, Momentum indicators, or exponential moving averages, are widely used across financial markets to identify behavioural biases. To date, TIs have never been applied to the forecasting of DAM prices. We demonstrate how the simple inclusion of TI features in DAM forecasting can significantly boost the regression accuracies of machine learning models; reducing the root mean squared errors of linear, ensemble, and deep model forecasts by up to 4.50%, 5.42%, and 4.09%, respectively. Moreover, tailored TIs are identified for each of these models, highlighting the added explanatory power offered by technical features.
Analysis, Design, and Experimental Research of a Novel Wheelchair-Stretcher Assistive Robot
Authors: Sang Lingfeng , Yamamura Masayuki , Dong Fangyan , Gan Zhongxue , Fu Jianzhong , Wang Hongbo , Tian Yu
Published: December 2019

Life care for disabled or semi-disabled elderly has become an increasingly common problem in society. In this paper, a novel wheelchair-stretcher assistive robot, which can meet the physiological needs of patients, is investigated and designed. The following tasks are conducted: (1) the mecanum wheel is adopted as the executive device of the walking mechanism, and its kinematics is analyzed in detail. (2) A five-link mechanism with single degree of freedom is proposed to realize the folding motion of the robot. Through the minimum conclusive area method, the optimal sizes of the armrests link and the side link are 507.9, and 332.5 mm, respectively. Based on the force analysis of the linkage mechanism, six torsion springs and RV (rotate vector) reduction motor are used as the driving device, which reduces the driving torque of the motor. (3) Based on the STM32 (STMicroelectronics 32-bits Microcontroller) chip, and combined with the theoretical analysis, the mechanical structure and the control system of the whole prototype are designed, and the feasibility of each module is verified by experimental research. The results confirm that the proposed robot has good performance, and that the control algorithm for the walking mechanism and the lifting mechanism are suitable.
Restrictions on the Use of Specimens Based on Magnesium and Titanium Nanocrystalline Oxides in Incidents Involving Uncontrolled Release of Hazardous Substances
Authors: Węsierski Tomasz , Majder-Łopatka Małgorzata , Walczak Agata
Published: December 2019

Undertaking effective and prompt action during chemical events is an important measure in preventing the spread of contamination. Studies have shown that despite very high surface activity of nanocrystalline metal oxides, their use does not reduce the fire hazard for hydrocarbon spills and alcohols more effectively than commonly used sorbents during fire rescue operations. Other limitations of specimens containing nanocrystalline oxides have also been proven, such as the maximum absorption similar to commonly used specimens, as well as high costs of conducting the acid neutralization process without increasing the level of safety of operations. Results obtained allowed the determination of proposed principles of effective and economically appropriate application of nanocrystalline metal oxides in rescue units.
Experimental Investigation on the Mechanical Properties of Curved Metallic Plate Dampers
Authors: Zheng Jie , Zhang Chunwei , Li Aiqun
Published: December 2019

This study proposes a novel curved steel plate damper to improve the seismic performance of structures. The theoretical analysis of the curved plate damper was carried out deriving formulas of key parameters of the curved plate damper including elastic lateral stiffness, yield strength, and yield displacement. Moreover, a cyclic loading test of four sets of specimens was conducted, and the hysteretic performance, ductility, energy dissipation performance, and strain of the specimens were studied. The results showed that the initial stiffness of the damper was large, no obvious damage was observed, and the hysteresis loop was full. The tested dampers had good deformation and energy dissipation performance. The stress variable rule of the damper was obtained by stress analysis, where the plastic deformation at the end of the semi-circular arc was large. The formula for various parameters of the damper was compared with experimental and numerical results; thus, the value of the adjustment coefficient was determined reasonable. Meanwhile, the rationality of the finite element model was also verified.
Numerical Analysis of the Cooling Performance in a 7.2 kW Integrated Bidirectional OBC/LDC Module
Authors: Noh Jung-Hun , Song Seong-il , Hur Deog-Jae
Published: December 2019

To satisfy increasing demands for ecofriendly vehicles, researchers are now studying electric vehicle (EV)-related technologies. In particular, integrated bidirectional onboard battery charger (OBC)/low-voltage DC–DC converter (LDC) modules are being researched to improve the efficiency of onboard chargers for EV charging applications. In this study, a numerical analysis method is proposed that considers the power loss and heat flow characteristics in the design of a 7.2 kW integrated bidirectional OBC/LDC module. The developed module supports four operating modes depending on the service situation: OBC and LDC single operation, OBC/LDC simultaneous operation, and LDC operation. The mode is selected based on the power system flow. The characteristics of the circuit were analyzed in each of the four modes to compute the heat loss from the major heating elements. The results of a numerical analysis of the internal cooling characteristics showed that the internal temperature was higher in the OBC single operating mode than in the OBC and LDC simultaneous operating mode in which the power loss was the highest. The results emphasize the importance of ensuring that cooling designs consider the characteristics of various modes as well as the worst-case power loss.
Indoor Particle Concentrations, Size Distributions, and Exposures in Middle Eastern Microenvironments
Authors: Hussein Tareq , Alameer Ali , Jaghbeir Omar , Albeitshaweesh Kolthoum , Malkawi Mazen , Boor Brandon E. , Koivisto Antti Joonas , Löndahl Jakob , Alrifai Osama , Al-Hunaiti Afnan
Published: December 2019

There is limited research on indoor air quality in the Middle East. In this study, concentrations and size distributions of indoor particles were measured in eight Jordanian dwellings during the winter and summer. Supplemental measurements of selected gaseous pollutants were also conducted. Indoor cooking, heating via the combustion of natural gas and kerosene, and tobacco/shisha smoking were associated with significant increases in the concentrations of ultrafine, fine, and coarse particles. Particle number (PN) and particle mass (PM) size distributions varied with the different indoor emission sources and among the eight dwellings. Natural gas cooking and natural gas or kerosene heaters were associated with PN concentrations on the order of 100,000 to 400,000 cm−3 and PM2.5 concentrations often in the range of 10 to 150 µg/m3. Tobacco and shisha (waterpipe or hookah) smoking, the latter of which is common in Jordan, were found to be strong emitters of indoor ultrafine and fine particles in the dwellings. Non-combustion cooking activities emitted comparably less PN and PM2.5. Indoor cooking and combustion processes were also found to increase concentrations of carbon monoxide, nitrogen dioxide, and volatile organic compounds. In general, concentrations of indoor particles were lower during the summer compared to the winter. In the absence of indoor activities, indoor PN and PM2.5 concentrations were generally below 10,000 cm−3 and 30 µg/m3, respectively. Collectively, the results suggest that Jordanian indoor environments can be heavily polluted when compared to the surrounding outdoor atmosphere primarily due to the ubiquity of indoor combustion associated with cooking, heating, and smoking.
Development of a Three-Dimensional Bioengineered Platform for Articular Cartilage Regeneration
Authors: Rubí-Sans Gerard , Recha-Sancho Lourdes , Pérez-Amodio Soledad , Mateos-Timoneda Miguel Ángel , Semino Carlos Eduardo , Engel Elisabeth
Published: December 2019

Degenerative cartilage pathologies are nowadays a major problem for the world population. Factors such as age, genetics or obesity can predispose people to suffer from articular cartilage degeneration, which involves severe pain, loss of mobility and consequently, a loss of quality of life. Current strategies in medicine are focused on the partial or total replacement of affected joints, physiotherapy and analgesics that do not address the underlying pathology. In an attempt to find an alternative therapy to restore or repair articular cartilage functions, the use of bioengineered tissues is proposed. In this study we present a three-dimensional (3D) bioengineered platform combining a 3D printed polycaprolactone (PCL) macrostructure with RAD16-I, a soft nanofibrous self-assembling peptide, as a suitable microenvironment for human mesenchymal stem cells’ (hMSC) proliferation and differentiation into chondrocytes. This 3D bioengineered platform allows for long-term hMSC culture resulting in chondrogenic differentiation and has mechanical properties resembling native articular cartilage. These promising results suggest that this approach could be potentially used in articular cartilage repair and regeneration.
Fabrication, Characterization and Application of Biomolecule Micropatterns on Cyclic Olefin Polymer (COP) Surfaces with Adjustable Contrast
Authors: Hager Roland , Haselgrübler Thomas , Haas Sandra , Lipp Anna-Maria , Weghuber Julian
Published: December 2019

Peptide and protein micropatterns are powerful tools for the investigation of various cellular processes, including protein–protein interactions (PPIs). Within recent years, various approaches for the production of functional surfaces have been developed. Most of these systems use glass as a substrate, which has several drawbacks, including high fragility and costs, especially if implemented for fluorescence microscopy. In addition, conventional fabrication technologies such as microcontact printing (µCP) are frequently used for the transfer of biomolecules to the glass surface. In this case, it is challenging to adjust the biomolecule density. Here, we show that cyclic olefin polymer (COP) foils, with their encouraging properties, including the ease of manufacturing, chemical resistance, biocompatibility, low water absorption, and optical clarity, are a promising alternative to glass substrates for the fabrication of micropatterns. Using a photolithography-based approach, we generated streptavidin/biotinylated antibody patterns on COPs with the possibility of adjusting the pattern contrast by varying plasma activation parameters. Our experimental setup was finally successfully implemented for the analysis of PPIs in the membranes of live cells via total internal reflection fluorescence (TIRF) microscopy.
EEG “Signs” of Verbal Creative Task Fulfillment with and without Overcoming Self-Induced Stereotypes
Authors: Shemyakina Natalia V. , Nagornova Zhanna V.
Published: December 2019

The study aimed to reveal task-related differences in story creation with and without the mental effort of overcoming self-induced stereotypes. Eighteen right-handed subjects (19.3 ± 1.1 years old) created stories. The subjects reported the formation of story plot stereotypes (as we call them: self-induced) during self-regulated creative production, which had to be overcome with the instruction to continue the story. Creative task fulfillment (without formed stereotypes—first stage of creation) was characterized by a decrease in the wave percentages of 9–10 Hz, 10–11 Hz and 11–12 Hz frequencies and EEG desynchronization (decreases in EEG spectral power) in the theta (4–8 Hz), alpha1 (8–10 Hz) and alpha2 (10–13 Hz) frequency bands in comparison with the REST (random episodic silent thought) state. The effortful creation task (with overcoming of self-induced stereotypes-second stage of creation) was characterized by increases in waves with frequencies of 9–10 Hz, 10–11 Hz, 11–12 Hz in temporal, occipital areas and pronounced EEG synchronization in alpha1,2 frequency bands in comparison with the free creation condition. It was also found, that the participants with the higher originality scores in psychological tests demonstrated increased percentage of high frequencies (11–12 Hz in comparison with those who had lower originality scores. Obtained results support the role of alpha and theta frequency bands dynamics in creative cognition.
Monitoring and Assessing the Dynamics of Building Deformation Changes in Landslide Areas
Authors: Florkowska Lucyna , Bryt-Nitarska Izabela , Gawałkiewicz Rafał , Kruczkowski Janusz
Published: December 2019

The paper presents the procedure and results of monitoring conducted by using a 3D measurement model, taking advantage of integrated surveying technologies developed for a building located within an activated landslide area. Geodynamic interactions within the building have resulted in a spatial deformation condition, leading to significant cracks of structure components and local basement floor upheavals. Conducted site research shows a reactivation of an old landslide form. To provide safe use conditions for the building, it was decided to monitor the structure and the area in its vicinity. Meeting this demand required developing an in-house monitoring system for the landslide form and the very structure. Measurements provided detailed information on the sizes and directions for the displacements of ground surface points and building structure points, as well as the dynamic properties of this phenomenon. Obtained results show the opportunity to use monitoring systems to acquire credible measurements data reflecting the real impact of ground landslide deformations on structures.
Loss of Ing3 Expression Results in Growth Retardation and Embryonic Death
Authors: Fink Dieter , Yau Tienyin , Nabbi Arash , Wagner Bettina , Wagner Christine , Hu Shiting Misaki , Lang Viktor , Handschuh Stephan , Riabowol Karl , Rülicke Thomas
Published: December 2019

The ING3 candidate tumour suppressor belongs to a family of histone modifying proteins involved in regulating cell proliferation, senescence, apoptosis, chromatin remodeling, and DNA repair. It is a stoichiometric member of the minimal NuA4 histone acetyl transferase (HAT) complex consisting of EAF6, EPC1, ING3, and TIP60. This complex is responsible for the transcription of an essential cascade of genes involved in embryonic development and in tumour suppression. ING3 has been linked to head and neck and hepatocellular cancers, although its status as a tumour suppressor has not been well established. Recent studies suggest a pro-metastasis role in prostate cancer progression. Here, we describe a transgenic mouse strain with insertional mutation of an UbC-mCherry expression cassette into the endogenous Ing3 locus, resulting in the disruption of ING3 protein expression. Homozygous mutants are embryonically lethal, display growth retardation, and severe developmental disorders. At embryonic day (E) 10.5, the last time point viable homozygous embryos were found, they were approximately half the size of heterozygous mice that develop normally. µCT analysis revealed a developmental defect in neural tube closure, resulting in the failure of formation of closed primary brain vesicles in homozygous mid-gestation embryos. This is consistent with high ING3 expression levels in the embryonic brains of heterozygous and wild type mice and its lack in homozygous mutant embryos that show a lack of ectodermal differentiation. Our data provide direct evidence that ING3 is an essential factor for normal embryonic development and that it plays a fundamental role in prenatal brain formation.
Molecular Genetics of Renal Cell Tumors: A Practical Diagnostic Approach
Authors: Alaghehbandan Reza , Perez Montiel Delia , Luis Ana Silvia , Hes Ondrej
Published: December 2019

Renal epithelial cell tumors are composed of a heterogeneous group of tumors with variable morphologic, immunohistochemical, and molecular features. A “histo-molecular” approach is now an integral part of defining renal tumors, aiming to be clinically and therapeutically pertinent. Most renal epithelial tumors including the new and emerging entities have distinct molecular and genetic features which can be detected using various methods. Most renal epithelial tumors can be diagnosed easily based on pure histologic findings with or without immunohistochemical examination. Furthermore, molecular-genetic testing can be utilized to assist in arriving at an accurate diagnosis. In this review, we presented the most current knowledge concerning molecular-genetic aspects of renal epithelial neoplasms, which potentially can be used in daily diagnostic practice.
CO2 Hydrogenation to Methanol over Ce and Zr Containing UiO-66 and Cu/UiO-66
Authors: Stawowy Michalina , Ciesielski Radosław , Maniecki Tomasz , Matus Krzysztof , Łużny Rafał , Trawczynski Janusz , Silvestre-Albero Joaquin , Łamacz Agata
Published: December 2019

Direct hydrogenation of CO2 to methanol is an interesting method to recycle CO2 emitted e.g., during combustion of fossil fuels. However, it is a challenging process because both the selectivity to methanol and its production are low. The metal-organic frameworks are relatively new class of materials with a potential to be used as catalysts or catalysts supports, also in the reaction of MeOH production. Among many interesting structures, the UiO-66 draws significant attention owing to its chemical and thermal stability, developed surface area, and the possibility of tuning its properties e.g., by exchanging the zirconium in the nodes to other metal cations. In this work we discuss—for the first time—the performance of Cu supported on UiO-66(Ce/Zr) in CO2 hydrogenation to MeOH. We show the impact of the composition of UiO-66-based catalysts, and the character of Cu-Zr and Cu-Ce interactions on MeOH production and MeOH selectivity during test carried out for 25 h at T = 200 °C and p = 1.8 MPa. Significant increase of selectivity to MeOH was noticed after exchanging half of Zr4+ cations with Ce4+; however, no change in MeOH production occurred. It was found that the Cu-Ce coexistence in the UiO-66-based catalytic system reduced the selectivity to MeOH when compared to Cu/UiO-66(Zr), which was ascribed to lower concentration of Cu0 active sites in Cu/UiO-66(Ce/Zr), and this was caused by oxygen spill-over between Cu0 and Ce4+, and thus, the oxidation of the former. The impact of reaction conditions on the structure stability of tested catalyst was also determined.
Origin of Monocytes/Macrophages Contributing to Chronic Inflammation in Chagas Disease: SIRT1 Inhibition of FAK-NFκB-Dependent Proliferation and Proinflammatory Activation of Macrophages
Authors: Wan Xianxiu , Chowdhury Imran Hussain , Jie Zuliang , Choudhuri Subhadip , Garg Nisha Jain
Published: December 2019

Background: Trypanosoma cruzi (Tc) causes Chagas disease (CD) that is the most frequent cause of heart failure in Latin America. TNF-α+ monocytes/macrophages (Mo/Mφ) are associated with inflammatory pathology in chronic CD. In this study, we determined the progenitor lineage of Mo/Mφ contributing to inflammation and examined the regulatory role of SIRT1 in modulating the Mo/Mφ response in Chagas disease. Methods and Results: C57BL/6 mice were infected with Tc, treated with SIRT1 agonist (SRT1720) after control of acute parasitemia, and monitored during chronic phase (150 days post-infection). Flow cytometry studies showed an increase in maturation of bone marrow hematopoietic stem cell (HSC)-derived Mo of proinflammatory and anti-inflammatory phenotype in acutely- and chronically-infected mice; however, these cells were not increased in splenic compartment of infected mice. Instead, yolk-sac-derived CD11b+ F4/80+ Mo/Mφ were increased in sinusoidal compartment of Chagas mice. The splenic CD11b+ F4/80+ Mo/Mφ of Chagas (vs. control) mice exhibited increased mRNA, protein, and surface expression of markers of proinflammatory phenotype (CD80+/CD64+ > CD200+/CD206+) associated with proinflammatory cytokines response (IL-6+TNF-α >> Arg-1+IL-10), and these were also detected in the myocardium of chronically infected mice. Infected mice treated with SRT1720 (vs. infected/untreated) exhibited decreased splenic expansion and myocardial infiltration of proinflammatory Mo/Mφ. SRT1720 did not alter the inherent capability of splenic Mo/Mφ of Chagas mice to respond to pathogen stimulus. Instead, SRT1720 dampened the Tc-induced increase in the expression and/or phosphorylation of focal adhesion kinase (FAK) and downstream transcription factors (Pu.1, c-Myb, and Runx1) involved in Mφ proliferation and migration and Notch1 involved in functional activation. Studies in cultured Mφ confirmed the agonistic effects of SIRT1 in controlling the Tc-induced, FAK-dependent increase in the expression of transcription factors and showed that SIRT1 agonist and FAK inhibitor abrogated the NF-κB transcriptional activity and inflammatory cytokine gene expression in Tc-infected Mφ. Conclusions: The proinflammatory Mo/Mφ of yolk sac origin drive the splenic and tissue inflammatory response in chronic CD. SRT1720 reprogrammed the Tc-induced FAK-dependent transcription factors involved in Mφ proliferation and proinflammatory activation in Chagas disease.
Cirrhotic Human Liver Extracellular Matrix 3D Scaffolds Promote Smad-Dependent TGF-β1 Epithelial Mesenchymal Transition
Authors: Mazza Giuseppe , Telese Andrea , Al-Akkad Walid , Frenguelli Luca , Levi Ana , Marrali Martina , Longato Lisa , Thanapirom Kessarin , Vilia Maria Giovanna , Lombardi Benedetta , Crowley Claire , Crawford Mark , Karsdal Morten A. , Leeming Diana J. , Marrone Giusi , Bottcher Katrin , Robinson Benjamin , Del Rio Hernandez Armando , Tamburrino Domenico , Spoletini Gabriele , Malago Massimo , Hall Andrew R. , Godovac-Zimmermann Jasminka , Luong Tu Vinh , De Coppi Paolo , Pinzani Massimo , Rombouts Krista
Published: December 2019

An altered liver microenvironment characterized by a dysregulated extracellular matrix (ECM) supports the development and progression of hepatocellular carcinoma (HCC). The development of experimental platforms able to reproduce these physio-pathological conditions is essential in order to identify and validate new therapeutic targets for HCC. The aim of this work was to validate a new in vitro model based on engineering three-dimensional (3D) healthy and cirrhotic human liver scaffolds with HCC cells recreating the micro-environmental features favoring HCC. Healthy and cirrhotic human livers ECM scaffolds were developed using a high shear stress oscillation-decellularization procedure. The scaffolds bio-physical/bio-chemical properties were analyzed by qualitative and quantitative approaches. Cirrhotic 3D scaffolds were characterized by biomechanical properties and microarchitecture typical of the native cirrhotic tissue. Proteomic analysis was employed on decellularized 3D scaffolds and showed specific enriched proteins in cirrhotic ECM in comparison to healthy ECM proteins. Cell repopulation of cirrhotic scaffolds highlighted a unique up-regulation in genes related to epithelial to mesenchymal transition (EMT) and TGFβ signaling. This was also supported by the presence and release of higher concentration of endogenous TGFβ1 in cirrhotic scaffolds in comparison to healthy scaffolds. Fibronectin secretion was significantly upregulated in cells grown in cirrhotic scaffolds in comparison to cells engrafted in healthy scaffolds. TGFβ1 induced the phosphorylation of canonical proteins Smad2/3, which was ECM scaffold-dependent. Important, TGFβ1-induced phosphorylation of Smad2/3 was significantly reduced and ECM scaffold-independent when pre/simultaneously treated with the TGFβ-R1 kinase inhibitor Galunisertib. In conclusion, the inherent features of cirrhotic human liver ECM micro-environment were dissected and characterized for the first time as key pro-carcinogenic components in HCC development.
A Septin Cytoskeleton-Targeting Small Molecule, Forchlorfenuron, Inhibits Epithelial Migration via Septin-Independent Perturbation of Cellular Signaling
Authors: Sun Lei , Cao Xuelei , Lechuga Susana , Feygin Alex , Naydenov Nayden G. , Ivanov Andrei I.
Published: December 2019

Septins are GTP-binding proteins that self-assemble into high-order cytoskeletal structures, filaments, and rings. The septin cytoskeleton has a number of cellular functions, including regulation of cytokinesis, cell migration, vesicle trafficking, and receptor signaling. A plant cytokinin, forchlorfenuron (FCF), interacts with septin subunits, resulting in the altered organization of the septin cytoskeleton. Although FCF has been extensively used to examine the roles of septins in various cellular processes, its specificity, and possible off-target effects in vertebrate systems, has not been investigated. In the present study, we demonstrate that FCF inhibits spontaneous, as well as hepatocyte growth factor-induced, migration of HT-29 and DU145 human epithelial cells. Additionally, FCF increases paracellular permeability of HT-29 cell monolayers. These inhibitory effects of FCF persist in epithelial cells where the septin cytoskeleton has been disassembled by either CRISPR/Cas9-mediated knockout or siRNA-mediated knockdown of septin 7, insinuating off-target effects of FCF. Biochemical analysis reveals that FCF-dependent inhibition of the motility of control and septin-depleted cells is accompanied by decreased expression of the c-Jun transcription factor and inhibited ERK activity. The described off-target effects of FCF strongly suggests that caution is warranted while using this compound to examine the biological functions of septins in cellular systems and model organisms.
Corrosion Behavior of the Ti–6Al–4V Alloy in Sulfate-Reducing Bacteria Solution
Authors: Zheng Xingwei , Zhuang Xin , Lei Yanhua , Chu Zhenhua , Xu Jingxiang , Gao Li , Sun Xiaoming
Published: December 2019

The corrosion behavior of the Ti–6Al–4V alloy was investigated in a sulfate-reducing bacteria (SRB) solution. The results showed that sulfate-reducing bacteria has good affinity with the surface of the Ti–6Al–4V alloy after 5 days. A potentiodynamic polarization test demonstrated that the corrosion resistance of the Ti–6Al–4V alloy was initially improved but deteriorated quickly in the subsequent period. The corrosion mechanism of the Ti–6Al–4V alloy was revealed by analyzing its microstructure with the aid of scanning electron microscopy, X-ray photoelectron spectroscopy and X-ray fluorescence. The pitting corrosion was deemed to be a typical cause of the corrosion behavior of the alloy in the SRB solution. The underlying mechanism of the pitting corrosion was proposed for the alloy.
A New Diagnostic Approach for Periprosthetic Acetabular Fractures Based on 3D Modeling: A Study Protocol
Authors: Marongiu Giuseppe , Prost Roberto , Capone Antonio
Published: December 2019

Periprosthetic acetabular fractures after total hip arthroplasty (THA) are mostly related to low energy trauma reduced bone quality. CT-scan is widely used to evaluate acetabular fractures, however, metal artifacts produced prosthetic implants limit the visualization of the articular surface and bone loss assessment. 3D modeling software allows us to creating tridimensional images of the bony surface, removing the metallic implants trough image segmentation. We highlight the use of 3D modeling and rapid prototyping (3D printing) for the diagnostic process of periprosthetic acetabular fracture around THA. 3D modeling software was used to improve the assessment of fracture morphology and bone quality. Moreover, the 3D images were printed in a real-life size model and used for preoperative implant templating, sizing and surgical simulation.
Recent Advances in the Pathophysiology of Musculocontractural Ehlers-Danlos Syndrome
Authors: Kosho Tomoki , Mizumoto Shuji , Watanabe Takafumi , Yoshizawa Takahiro , Miyake Noriko , Yamada Shuhei
Published: December 2019

Musculocontractural Ehlers–Danlos Syndome (mcEDS) is a type of EDS caused by biallelic pathogenic variants in the gene for carbohydrate sulfotransferase 14/dermatan 4-O-sulfotransferase 1 (CHST14/D4ST1, mcEDS-CHST14), or in the gene for dermatan sulfate epimerase (DSE, mcEDS-DSE). Thus far, 41 patients from 28 families with mcEDS-CHST14 and five patients from four families with mcEDS-DSE have been described in the literature. Clinical features comprise multisystem congenital malformations and progressive connective tissue fragility-related manifestations. This review outlines recent advances in understanding the pathophysiology of mcEDS. Pathogenic variants in CHST14 or DSE lead to reduced activities of relevant enzymes, resulting in a negligible amount of dermatan sulfate (DS) and an excessive amount of chondroitin sulfate. Connective tissue fragility is presumably attributable to a compositional change in the glycosaminoglycan chains of decorin, a major DS-proteoglycan in the skin that contributes to collagen fibril assembly. Collagen fibrils in affected skin are dispersed in the papillary to reticular dermis, whereas those in normal skin are regularly and tightly assembled. Glycosaminoglycan chains are linear in affected skin, stretching from the outer surface of collagen fibrils to adjacent fibrils; glycosaminoglycan chains are curved in normal skin, maintaining close contact with attached collagen fibrils. Homozygous (Chst14−/−) mice have been shown perinatal lethality, shorter fetal length and vessel-related placental abnormalities. Milder phenotypes in mcEDS-DSE might be related to a smaller fraction of decorin DS, potentially through residual DSE activity or compensation by DSE2 activity. These findings suggest critical roles of DS and DS-proteoglycans in the multisystem development and maintenance of connective tissues, and provide fundamental evidence to support future etiology-based therapies.