bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementation of Highly Challenging Balance Training for Parkinson’s disease in Clinical Practice: A Process Evaluation
Authors: Leavy Breiffni , Joseph Conran , Kwak Lydia , Franzén Erika
Published: January 2021

Background: Process evaluations provide contextual insight into the way in which interventions are delivered. This information is essential when designing strategies to implement programs into wider clinical practice. We performed a process evaluation of the HiBalance effectiveness trial investigating the effects of a 10-week of highly challenging and progressive balance training for mild-moderate Parkinson’s disease (PD). Study aims were to investigate i) the quality and quantity of intervention delivery and ii) barriers and facilitators for implementation. Methods: Process outcomes included; Fidelity; Dose (delivered and received) Recruitment and Reach. Investigation of barriers and facilitators was guided by the Consolidated Framework for Implementation Research. Program delivery was assessed across four neurological rehabilitation sites during a two-year period. Data collection was mixed-methods in nature and quantitative and qualitative data were merged during the analysis phase. Results : Thirteen program trainers delivered the intervention to 12 separate groups during 119 training sessions. Trainer fidelity to program core components was very high in 104 (87%) of the sessions. Participant responsiveness to the core components was generally high, although adherence to the home exercise program was low (50%). No significant context-specific differences were observed across sites in terms of fidelity, dose delivered/ received or participant characteristics, despite varying recruitment methods. Facilitators to program delivery were; PD-specificity, high training frequency and professional autonomy. Perceived barriers included; cognitive impairment, absent reactional balance among participants, as well a heterogeneous group in relation to balance capacity. Conclusion: These findings provide corroborating evidence for outcome evaluation results and valuable information for the further adaptation and implementation of this program. Important lessons can also be learned for researchers and clinicians planning to implement challenging exercise training programs for people with mild-moderate PD. Trial registration: ClinicalTrials.gov, NCT02727478, registered 30 march 2016 − Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT02727478
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in-vitro study
Authors: Tambone Erica , Bonomi Emiliana , Ghensi Paolo , Maniglio Devid , Ceresa Chiara , Agostinacchio Francesca , Caciagli Patrizio , Nollo Giandomenico , Piccoli Federico , Caola Iole , Fracchia Letizia , Tessarolo Francesco
Published: January 2021

Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods: R89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5). using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy. Results: R89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 99%, 47% and 7% and of S. epidermidis by 54%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 78% for S. epidermidis at 24 h. Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.
The impact of a training programme incorporating the conceptual framework of the International Classification of Functioning (ICF) on knowledge and attitudes regarding interprofessional practice in Rwandan health professionals: A cluster randomized control trial
Authors: Sagahutu Jean Baptiste , Kagwiza Jeanne , Cilliers Francois , Jelsma Jennifer
Published: January 2021

Background: The first step in improving interprofessional teamwork entails training health professionals (HP) to acknowledge the role and value the contribution of each member of the team. The International Classification of Functioning, Disability and Health (ICF) has been developed by WHO to provide a common language to facilitate communication between HPs. Objective: To determine whether ICF training programme would result in improved knowledge and attitudes regarding interprofessional practice within Rwandan district hospitals.Design, setting and participants: A cluster randomised, single blinded, control trial design was used to select four district hospitals. Participants included physicians, social workers, physiotherapists, nutritionists, clinical psychologists/mental health nurses. Intervention: Health professionals either received one day’s training in interprofessional practice (IPP) based on the ICF (experimental group) as a collaborative framework or a short talk on the topic (control group).Outcome measures: Validated questionnaires were used to explore changes in knowledge and attitudes. Ethical approval was obtained from the relevant authorities. Results: There were 103 participants in the experimental and 100 in the control group. There was no significant difference between Knowledge and Attitude scales at baseline. Post-intervention the experimental group (mean=41.3, SD=9.5) scored significantly higher on the knowledge scale than the control group (mean=17.7, SD=4.7 (t=22.5; p<.001)). The median scores on the Attitude Scale improved in the Experimental group from 77.8 to 91.1%, whereas the median scores of the control remained approximately 80% (Adjusted Z =10.72p<.001). Conclusion: The ICF proved to be a useful framework for structuring the training of all HPs in IPP and the training resulted in a significant improvement in knowledge and attitudes regarding IPP. As suggested by the HPs, more training and refresher courses were needed for sustainability and the training should be extended to other hospitals in Rwanda. It is thus recommended that the framework can be used in interprofessional education and practice in Rwanda and possibly in other similar countries.Key works: Interprofessional, ICF, Rwanda, District Hospital.Name of the registry: Pan African Clinical Trial Registry Trial registration number: PACTR201604001185358 Date of registration: 22/04/2016URL of trial registry record: www.pactr.orgFunding: Republic of Rwanda through Rwanda Education Board
Effect of a 980-nm diode laser on post-operative pain after endodontic treatment in teeth with apical periodontitis: A randomized clinical trial
Authors: Kaplan Tuna , Sezgin Güzide Pelin , Kaplan Sema Sönmez
Published: January 2021

Background: This study aimed to assess the effect of a 980-nm diode laser following chemomechanical root canal preparation on the severity of post-operative pain (PP) after root canal treatment (RCT). Methods: This study included asymptomatic, single-rooted teeth with a periapical index (PAI) score of 3 or 4. All the patients received RCT, including dressing with calcium hydroxide (Ca(OH) 2 ), during two visits. The patients were randomly divided into two groups ( n: 30): a control group, in which the final irrigation was performed using 5 ml of 2.5% sodium hypochlorite (NaOCI), followed by 5 ml of 17% and ethylenediaminetetraacetic acid (EDTA) and 5 ml of distilled water, and a laser group, in which the root canals were irradiated using a 980-nm diode laser after the final irrigation at both visits. The pain levels after both visits were evaluated using a visual analogue scale (VAS) after 8 h, 24 h, 48 h and 7 d. In addition, the time intervals to medication intake were recorded. The collected data were statistically analysed using the chi-square and Mann–Whitney U test ( p <0.05).Results: The average pain level in the control group 24 h after the first visit was significantly higher than that in the laser group ( p <0.05). The average pain level 24 h and 48 h after the second visit was significantly higher in the control group ( p <0.05). The levels of PP 24 h after the first visit were higher than those after the second visit only in the control group ( p <0.05). After the first visit, analgesic use in the control group was significantly higher after 8 h (40%) and 24 h (23%) as compared with that in the laser group ( p <0.05).Conclusions: Root canal irradiation with a diode laser may reduce PP after RCT in single-rooted teeth with a PAI score of 3 or 4. Keywords: diode laser, irrigation, post-operative pain, root canal treatment, visual analogue scaleTrial registration: Effect of the Diode Laser on Post-operative Pain After Endodontic Treatment in Teeth with Apical Periodontitis: NCT04486196. Registered 24 July 2020 - Retrospectively registered, http://clinicaltrials.gov/ct2/show/NCT04486196
LncRNA CBR3-AS1 regulates of breast cancer drug sensitivity as a competing endogenous RNA through the JNK1/MEK4‑mediated MAPK signal pathway
Authors: Zhang Ming , Wang Yan , Jiang Longyang , Song Xinyue , Zheng Ang , Gao Hua , Wei Minjie , Zhao Lin
Published: January 2021

Background: Adriamycin (ADR) resistance is one of the main obstacles to improving the clinical prognosis of breast cancer patients. Long noncoding RNAs (lncRNAs) can regulate cell behavior, but the role of these RNAs in the anti-ADR activity of breast cancer remains unclear. Here, we aim to investigate the imbalance of a particular long noncoding RNA, lncRNA CBR3 antisense RNA 1 (CBR3-AS1), and its role in ADR resistance.Methods: Microarray analysis of ADR-resistant breast cancer cells was performed to identify CBR3-AS1. CCK-8 and colony formation assays were used to detect the sensitivity of breast cancer cells to ADR. Dual-luciferase reporter, RNA pulldown, IHC and western blot analyses were used to verify the relationship between the expression of CBR3-AS1, miRNA and target genes. For in vivo experiments, the effect of CBR3-AS1 on breast cancer resistance was observed in a xenograft tumor model. The role of CBR3-AS1 in influencing ADR sensitivity was verified by clinical breast cancer specimens from the TCGA, CCLE, and GDSC databases.Results: We found that CBR3-AS1 expression was significantly increased in breast cancer tissues and was closely correlated with poor prognosis. CBR3-AS1 overexpression promoted ADR resistance in breast cancer cells in vitro and in vivo. Mechanistically, we identified that CBR3-AS1 functioned as a competitive endogenous RNA by sponging miR-25-3p. MEK4 and JNK1 of the MAPK pathway were determined to be direct downstream proteins of the CBR3-AS1/miR-25-3p axis in breast cancer cells.Conclusions: In summary, our findings demonstrate that CBR3-AS1 plays a critical role in the chemotherapy resistance of breast cancer by mediating the miR-25-3p and MEK4/JNK1 regulatory axes. The potential of CBR3-AS1 as a targetable oncogene and therapeutic biomarker of breast cancer was identified.
A new candidate vaccine for human brucellosis based on influenza viral vectors: a preliminary investigation for the development of an immunization schedule in a guinea pig model
Authors: Bugybayeva Dina , Kydyrbayev Zhailaubay , Zinina Nadezhda , Assanzhanova Nurika , Yespembetov Bolat , Kozhamkulov Yerken , Zakarya Kunsulu , Ryskeldinova Sholpan , Tabynov Kaissar
Published: January 2021

Background: A new candidate vector vaccine against human brucellosis based on recombinant influenza viral vectors (rIVV) subtypes H5N1 expressing Brucella outer membrane protein (Omp) 16, L7/L12, Omp19 or Cu-Zn SOD proteins has been developed. This paper presents the results of the study of protection of the vaccine using on guinea pigs, including various options of administering, dose and frequency. Provided data of the novel vaccine candidate will contribute to its further movement into the preclinical stage study. Methods: General states of guinea pigs was assessed based on behavior and dynamics of a guinea pig weight-gain test. The effectiveness of the new anti-brucellosis vector vaccine was determined by studying its protective effect after conjunctival, intranasal and sublingual administration in doses 105 EID50, 106 EID50 and 107 EID50 during prime and boost vaccinations of animals, followed by challenge with a virulent strain of B. melitensis 16M infection. For sake of comparison, the commercial B. melitensis Rev.1 vaccine was used as a control. The protective properties of vaccines were assessed by quantitation of Brucella colonization in organs and tissues of infected animals and compared to the control groups.Results: It was observed a gradual increase in body weight of guinea pigs after prime and booster immunization with the vaccine using conjunctival, intranasal and sublingual routes of administration, as well as after using various doses of vaccine. The most optimal way of using the vaccine has been established: double intranasal immunization of guinea pigs at a dose of 106 EID50, which provides 80% protection of guinea pigs from B. melitensis 16M infection (P<0.05), which is comparable to the results of the effectiveness of the commercial B. melitensis Rev.1 vaccine.Conclusions: We developed effective vaccine candidate against brucellosis and developed its immunization protocol in guinea pig model. We believe that because of these studies, the proposed vaccine has achieved the best level of protection, which in turn provides a basis for its further promotion.
Clinical characteristics of refractory Mycoplasma pneumoniae pneumonia in children treated with glucocorticoid pulse therapy
Authors: Zhu Zhenli , Zhang Tongqiang , Guo Wei , Ling Yaoyao , Tian Jiao , Xu Yongsheng
Published: January 2021

Background: To observe the effect of corticosteroids in the treatment of children with refractory Mycoplasma pneumoniae pneumonia (RMPP) under different doses, to summarize the clinical features of children treated with glucocorticoid pulse therapy.Methods: The clinical data of 125 children with RMPP hospitalized in Tianjin Children's Hospital from September 2018 to October 2019 were retrospectively analyzed. They were divided into two groups according to the dose of hormone. Compare the clinical features, laboratory findings, and imaging between the two groups, and use meaningful related indicators as ROC curves to find reference indicators for pulse therapy.Results: (1)The median age of the group II was older than that of the group I(P<0.05).(2)We found more severe presentations, higher incidence of extra-pulmonary complications and more serious radiological findings in group II, which needed oxygen more often, higher the hormone, higher usage rate of gamma globulin, higher usage rate of bronchoscopy, and higher incidence of plastic bronchitis(P<0.05).(3)WBC, CRP, LDH, FER, D-D dimer, APTT, TT, PCT, IL-6 and the percentage of neutrophils in peripheral blood in Group II were higher than those in Group I(P<0.05).(4)In ROC curve analysis, CRP, LDH, FER, and neutrophils of leukocyte classification were independent related factors that could be used as valuable predictors of methylprednisolone pulse therapy for RMPP in children. The cut-off values were CRP44.45mg/L, LDH590IU/L, FER411ng/L, and neutrophils in leukocyte classification were 73.75%, respectively.Conclusion: CRP≥44.45mg/L, LDH≥590IU/L, FER≥411ng/L, neutrophil≥73.75%, lung consolidation and pleural effusion are found in RMPP patients, which could be treated with pulse dose of methylprednisolone in time to reduce the incidence of severe RMPP and the occurrence of severe sequelae.
Genome-wide identification, characterization, and expression analysis of tea plant autophagy-related genes (CsARGs) demonstrates that they play diverse roles during development and under abiotic stress
Authors: Wang Huan , Ding ZhaoTang , Gou Mengjie , Hu Jianhui , Wang Yu , Wang Lu , Wang Yuchun , Di Taimei , Zhang Xinfu , Hao Xinyuan , Wang Xinchao , Yang Yajun , Qian Wenjun
Published: January 2021

Background: Autophagy, meaning ‘self-eating’, is required for the degradation and recycling of cytoplasmic constituents under stressful and non-stressful conditions, which helps to maintain cellular homeostasis and delay aging and longevity in eukaryotes. To date, the functions of autophagy have been heavily studied in yeast, mammals and model plants, but few studies have focused on economically important crops, especially tea plants (Camellia sinensis). The roles played by autophagy in coping with various environmental stimuli have not been fully elucidated to date. Therefore, investigating the functions of autophagy-related genes in tea plants may help to elucidate the mechanism governing autophagy in response to stresses in woody plants.Results: In this study, we identified 35 C. sinensis autophagy-related genes (CsARGs). Each CsARG is highly conserved with its homologues from other plant species, except for CsATG14. Tissue-specific expression analysis demonstrated that the abundances of CsARGs varied across different tissues, but CsATG8c/i showed a degree of tissue specificity. Under hormone and abiotic stress conditions, most CsARGs were upregulated at different time points during the treatment. In addition, the expression levels of 10 CsARGs were higher in the cold-resistant cultivar ‘Longjing43’ than in the cold-susceptible cultivar ‘Damianbai’ during the CA period; however, the expression of CsATG101 showed the opposite tendency.Conclusions: We performed a comprehensive bioinformatic and physiological analysis of CsARGs in tea plants, and these results may help to establish a foundation for further research investigating the molecular mechanisms governing autophagy in tea plant growth, development and response to stress. Meanwhile, some CsARGs could serve as putative molecular markers for the breeding of cold-resistant tea plants in future research.
Chloroplast Genome Variation and Phylogenetic Relationships of Atractylodes species
Authors: Wang Yiheng , Wang Sheng , Liu Yanlei , Yuan Qingjun , Sun Jiahui , Guo Lanping
Published: January 2021

Background: Atractylodes DC is the basic original plant of the widely used herbal medicines “Baizhu” and “Cangzhu” and an endemic genus in East Asia. Species within the genus have minor morphological differences, and the universal DNA barcodes cannot clearly distinguish the systemic relationship or identify the species of the genus. In order to solve these question, we sequenced the chloroplast genomes of all species of Atractylodes using high-throughput sequencing.Results: The results indicate that the chloroplast genome of Atractylodes has a typical quadripartite structure and ranges from 152,294 bp (A. carlinoides) to 153,261 bp (A. macrocephala) in size. The genome of all species contains 113 genes, including 79 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Four hotspots, rpl22-rps19-rpl2, psbM-trnD, trnR-trnT(GGU), and trnT(UGU)-trnL, and a total of 42-47 simple sequence repeats (SSR) were identified as the most promising potentially variable makers for species delimitation and population genetic studies. Phylogenetic analyses of the whole chloroplast genomes indicate that Atractylodes is a clade within the tribe Cynareae; Atractylodes species form a monophyly that clearly reflects the relationship within the genus. Conclusions: Our study included investigations of the sequences and structural genomic variations, phylogenetics and mutation dynamics of Atractylodes chloroplast genomes and will facilitate future studies in population genetics, taxonomy and species identification.
Hepatic safety of repeated treatment with pyronaridine-artesunate versus artemether-lumefantrine in patients with uncomplicated malaria: a secondary analysis of the WANECAM 1 data from Bobo-Dioulasso, Burkina Faso
Authors: Compaoré Yves Daniel , Zongo Issaka , Somé Anyirékun F. , Barry Nouhoun , Nikiema Frederick , Kabore Talato N. , Aminata Ouattara , Kabre Zachari , Wermi Kadidiatou , Zongo Moussa , Yerbanga Rakiswende S. , Sagara Issaka , Djimdé Abdoulaye , Ouédraogo Jean Bosco
Published: January 2021

BackgroundThe use of pyronaridine-artesunate (PA) has been associated with scarce transaminitis in patients. This analysis aimed to evaluate the hepatic safety profile of repeated treatment with PA versus artemether-lumefantrine (AL) in patients with consecutive uncomplicated malaria episodes in Bobo-Dioulasso, Burkina Faso. MethodsThis study analysed data from a clinical trial conducted from 2012 to 2015, in which participants with uncomplicated malaria were assigned to either PA or AL arms and followed up to 42 days. Subsequent malaria episodes within a 2-years follow up period were also treated with the same ACT initially allocated. Transaminases (AST/ALT), alkaline phosphatase (ALP), total and direct bilirubin were measured at days 0 (baseline), 3, 7, 28 and on some unscheduled days if required. The proportions of non-clinical hepatic adverse events (AEs) following first and repeated treatments with PA and AL were compared within study arms. The association of these AEs with retreatment in each arm was also determined using a logistic regression model. ResultsA total of 1379 malaria episodes were included in the intention to treat analysis with 60% of all cases occurring in the AL arm. Overall, 179 non-clinical hepatic AEs were recorded in the AL arm versus 145 in the PA arm. Elevated ALT was noted in 3.05% of treated malaria episodes, elevated AST 3.34%, elevated ALP 1.81%, and elevated total and direct bilirubin in 7.90% and 7.40% respectively. Retreated participants were less likely to experience elevated ALT and AST than first episode treated participants in both arms. One case of Hy’s law condition was recorded in a first treated participant of the PA arm. Participants from the retreatment group were 76% and 84% less likely to have elevated ALT and AST, respectively, in the AL arm and 68% less likely to present elevated ALT in the PA arm. In contrast, they were almost 2 times more likely to experience elevated total bilirubin in both arms.ConclusionPyronaridine-artesunate and artemether-lumefantrine showed similar hepatic safety when used repeatedly in participants with uncomplicated malaria. Pyronaridine-artesunate represents therefore a suitable alternative to the current first line anti-malarial drugs in use in endemic areas.
“Those are things for married people” Exploring parents’/adults’ and adolescents’ perspectives on contraceptives in Narok and Homa Bay Counties, Kenya
Authors: Mwaisaka Jefferson , Wado Yohannes Dibaba , Ouedraogo Ramatou , Oduor Clement , Habib Helen , Njagi Joan , Bangha Martin
Published: January 2021

BackgroundContraceptive use among adolescent girls is low in many sub-Saharan African countries including Kenya. Attitude and perspectives about contraception of community members including adolescent girls themselves may be likely to limit contraceptive use among adolescent girls. This study was conducted to explore and compare adults’/parents’ and adolescent girls’ narratives and perspectives about contraception in Narok and Homa Bay counties, Kenya.MethodsQualitative data from 45 in-depth-interviews conducted with purposively selected consenting adolescent girls aged 15-19 was used. Additionally, twelve focus group discussions were held with 86 consenting adults conveniently recruited from the two counties. All discussions were conducted in the local language and audio recorded following consent of the study participants. Female moderators were engaged throughout the study making it appropriate for the study to solicit feedback from the targeted respondents.ResultsFindings highlighted adults’ perceptions on adolescents’ sexuality and the presence of stringent conceptions about the side-effects of contraception in the study communities. Some participants underscored the need for open contraceptive talk between parents and their adolescent girls. Four main themes emerged from the discussions; i) Perceptions about adolescents’ sexuality and risk prevention, ii) Conceptions about contraception among nulligravida adolescents: fear of infertility, malformation and sexual libertinism, iii) Post-pregnancy contraceptive considerations and iv) Thinking differently: divergent views regarding contraceptives and parent/adolescent discussion.ConclusionsOur findings suggest the need for increased attention towards adolescents and their caregivers particularly in demystifying contraceptive misconceptions. Programmatic responses and models which include the provision of comprehensive sexuality education and increased access to and utilization of SRH information, products and services through a well-informed approach need to be well executed. Programmatic efforts like SRH community education should further seek to enhance the capacity of parents to discuss sexuality with their adolescents.
Electricity generation and pollutants removal of landfill leachate by osmotic microbial fuel cells with different forward osmosis membranes
Authors: Jiang Nan , Huang Li , Huang Manhong , Cai Teng , Song Jialing , Zheng Shengyang , Guo Jili , Kong Zhuang , Chen Liang
Published: January 2021

In this study, thin-film composite with embedded polyester screen, cellulose triacetate with a cast nonwoven and cellulose triacetate with embedded polyester screen (CTA-ES) were examined as the intermediate membranes in osmotic microbial fuel cells (OsMFCs). The reactors were fed with actual landfill leachate and the performance was studied in two operation modes: active layer facing draw solution and active layer facing feed solution (AL-FS). The OsMFC with CTA-ES exhibited the best energy generation (maximum power density: 0.44 W m-2) and pollutant removal efficiency (ammonia nitrogen: 70.12 ± 0.28%, total nitrogen: 74.04 ± 0.33%) in the AL-FS mode, which could be ascribed to the lowest internal resistance (236.75 ohm) and highest microbial richness. Pseudomonas was the highest proportion of microbial in OsMFCs. The results of this study has demonstrated the potential of OsMFCs for landfill leachate treatment.
Rapid ethnographic assessment for potential anti-malarial mass drug administration in an outbreak area of Santo Domingo, Dominican Republic
Authors: Keys Hunter , Ureña Keyla , Reyes Jhefres , Bardosh Kevin , Pell Christopher , Puello Jose , Blount Stephen , Noland Gregory S.
Published: January 2021

Background In the Dominican Republic, a recent outbreak of malaria in the capital, Santo Domingo, threatens efforts to eliminate the disease. Mass drug administration (MDA) has been proposed as one strategy to reduce transmission. The success of MDA is contingent upon high levels of acceptance among the target population. To inform the design of future MDA campaigns, this rapid ethnographic assessment examined malaria-related knowledge and attitudes toward malaria MDA among residents of a transmission focus in Santo Domingo.Methods In October 2019, a rapid ethnographic assessment was conducted in the Los Tres Brazos transmission focus, which had not previously received MDA. National malaria programme staff conducted 61 structured interviews with key informants, recorded observations, and held 72 informal conversations. Using a grounded theory approach, data were analysed during three workshop sessions with research team members. Results Among those who had heard of malaria in the structured interviews (n=39/61; 64%), understanding of the disease was largely based on personal experience from past outbreaks or through word-of-mouth. Community health workers (promotores) were trusted for health information and malaria diagnosis more so than professional clinicians. No participant (0%) was familiar with malaria MDA. After learning about MDA, almost all study participants (92%) said that they would participate, seeing it as a way to care for their community. Reasons for not participating in future MDA included not trusting drug administrators, feeling reluctant to take unprescribed medicine, and fear of missing work. Additional identified challenges to MDA included reaching specific demographic groups, disseminating effective MDA campaign messages, and managing misinformation and political influence. Conclusion Residents appear accepting of MDA despite a lack of prior familiarity. Successful MDA will depend on several factors: fostering relationships among community-based health workers, clinicians, community leaders, and others; developing clear health messages that use local terms and spreading them through a variety of media and social networks; and contextualizing MDA as part of a broader effort to promote community health.
Postoperative Analgesic Efficiency of Quadratus Lumborum Block Iii Versus Peritubal Local Infiltration in Patients Undergoing Percutaneous Nephrolithotomy Operation: A Randomized Comparative Trial
Authors: Armanious Simon Halim , Youssef Peter M. , Kamal Manal , Kady Galal El , Abdelrahman Tamer N.
Published: January 2021

Background: The postoperative pain after percutaneous nephrolithotomy (PCNL) can be managed by many peripheral blocks techniques (e.g., paravertebral and quadrates lumborum block), and local anesthesia infiltration. Therefore, this prospective study was conducted to evaluate the effect of quadratus lumborum type III versus peritubal local infiltrating on postoperative analgesia for patients undergoing PCNL under general anesthesia.Methods: Eligible subjects after exclusion of COVID-19 patients, with renal stones less than 2 cm undergoing PCNL surgery under general anesthesia. Exclusion criteria were: 21 60 years of age; bleeding disorders; allergies to local anesthetics; psychiatric patients; and American Society of Anesthesia (ASA) physical status III and IV. Eighty patients were randomly allocated to 2 equal groups. In the Quadratus group, participant received 20 mL of bupivacaine 0.25% in the fascial plane between the quadratus lumborum and Psoas muscle; and in the peritubal group, patients received 10 mL of bupivacaine 0.25% around the nephrostomy tube at 6 and 12 o’clock positions (total 20 mL). The quadratus lumborum block and peritubal infiltration were performed by the primary investigator and the urologist, respectively. All patients received standard postoperative analgesia (paracetamol, ketorolac, and morphine via patient-controlled analgesia. Both patients and Data collecting staff were blinded to study group. Primary outcome was the visual analogue score at rest and on coughing or movement at 2, 4, 6, 12, and 24 hours. Secondary outcomes were 24 hours total morphine consumption, 1st use of morphine pump, incidence of side effects (pruritis and nausea and vomiting) and patient satisfaction.Results: Data were analyzed from 78 patients (39 patients in each group). The mean resting VAS score in quadratus group at 6 and 12 hours was (3), while the mean VAS in peritubal group was (5) and (6) at 6 and 12 hours, respectively. Dynamic VAS score in peritubal group at 6 hours was (mean 6) with p<0.0001 when compared to resting value. The 24 hours’ morphine consumption was 8.5-11mg and 16-19 mg, in quadratus and peritubal group, respectively (the difference in mean of 9 at the 95% confidence interval; p<0.0001). patients start to use morphine pump after 121.4 min and 180.2 min in group peritubal and quadratus, respectively. Patients in group quadratus were statistically more satisfied, in both groups incidence of complication was low.Conclusion: Quadratus Lumborum block was more effective than Peritubal LA infiltration regarding postoperative VAS score both at rest and during movement with lower postoperative morphine consumption and more satisfaction in patients undergoing PCNL surgery.
THE CONCEPT OF ECONOMIC SECURITY OF THE STATE AS AN OBJECT OF ADMINISTRATIVE AND LEGAL PROTECTION: THEORETICAL AND METHODOLOGICAL ASPECTS
Authors: Melnik V.I.
Published: January 2021

The article is devoted to the problems of studying and researching the concept of economic security of the state as an object of administrative and legal protection. Attention is drawn to the fact that one of the important tasks in modern administrative
law is the question of defining the concept of a particular subject, process, thing, or another object of scientific interest. It is believed that this will help to avoid various often logical formulations of it, likely to develop unified approaches to understanding its essence, in particular, and for possible further scientific research on improving the existing state system of ensuring the economic security of Ukraine, etc.
It is emphasized on the undisputed importance of all legal assistance to the proper functioning of the economic security of the state. First of all, it was emphasized that it is connected with the desire for real independent further development of the country in the conditions of complex political and legal transformations and territorial changes in the recent past, as well as the dynamic course of active globalization processes and strengthening of the tangible destructive influence of existing and emergence.
new devastating threats to the national economy in the present day, and obviously in the short term.
It was established that the need for economic security was declared in the Basic Law and other documents that are important for the Ukrainian state. These acts also define (define) the range of subjects authorized to implement such a policy of the state, their competence, rights and obligations, legal guarantees, etc.
At the same time, it is emphasized that the problem of defining the concept of economic security is important, given its multidimensionality, the lack of interpretation of the term precisely in the legislative documents, and, of course, its overstated importance for the modern country. Considering the priority role of the state in the issue of ensuring the economic security of Ukraine, it is pointed out that it is advisable to formulate such a definition in terms of administrative and legal science.
In order to qualitatively solve the set scientific problem, the views of leading domestic and foreign scientists and scholars of the past and present are presented, as well as the relevant national legal framework is being worked out. Taking into account current legal realities, the opinions of scientists and scientists suggest the author’s interpretation of the concept of “economic security of the state”, just as an object of administrative and legal protection.
Keywords: administrative and legal protection, security, the economic security of the state, the concept of economic security of the state, the concept of economic security of the state as an object of administrative and legal protection.
INTERNATIONAL STANDARDS OF STATE FINANCIAL CONTROL
Authors: Kulish A. M. , Ragulina Y. M.
Published: January 2021

In this work, the authors have examined the concept of “standards” and “standardization” relating to public finance and public financial control. It was recognized that national financial systems activities should be based on generally accepted international standards in order to enhance professionalism and efficiency. Attention was paid to the peculiarities of national standards of the United States of America and European Union countries, in particular the United Kingdom of Great Britain, Lithuania, Georgia. As part of the analysis of the Association Agreement on Public Internal Financial Control, it was determined that cooperation between Ukraine and the European Union, the European Atomic Energy Community, and their Member States takes place through harmonization with the following international standards: the Institute of Internal Auditors (EA IIA), The International Federation of Accountants
(IFAC) and International Organisation of Supreme Audit Institutions (INTOSAI). Much attention was paid to the concept of public internal financial control developed by the European Commission to provide a viable model capable of assisting national governments in revising internal controls in the field of finance and modernizing them in accordance with international standards and best practice in countries- members of the European Union. The scope of the aforementioned concept, its essence, its main elements, and features was determined. The standards of the international organization INTOSAI were analyzed, containing the guiding principles of the audit activity, the peculiarities of their implementation and direction; the activities of the International Professional Association of Internal Auditors EC IIA (European Confederation of Institutes of Internal Auditing); standards developed by the International Federation of Accountants (IFAC); the activities of the Committee of Sponsoring Organizations of the Treadway Commission COSO.
In addition, the article identified the essential elements of an efficient and effective internal financial control system.

Keywords: standardization, state financial control, international standards.
FELDSHER-OBSTETRIC POINTS WITHIN THE FRAMEWORK OF MEDICAL REFORM
Authors: Pavlenko B.O. , Kravchenko L.L.
Published: January 2021

The article examines the most urgent problems of medical-midwifery points that arose as a result of medical reform. An important element in the effective operation of midwives is their legal support.
The importance of scientific research on the consequences of medical reform for midwives is explained by the following elements:
– first, one-third of the population of Ukraine lives in rural areas and does not receive proper medical services, as paramedics and obstetric centers have ceased to serve as the primary link of the health care system, and have become an intermediate point between the patient and the clinic;
– secondly, the demographic situation of the villages, and consequently Ukraine, depends on the activity of the midwifery-midwifery centers, since the lack of medical centers in the rural areas causes the extinction of the villages: an increasing number of people move to other settlements, some patients may simply not be able to wait. providing emergency medical care.
Therefore, there is a need to investigate changes in the activities of the midwifery units after the introduction of the primary health care reform. Therefore, the creation of a legal framework that will include the rights and responsibilities of midwives and midwives, and clearly regulate their activities, will allow these medical centers to work effectively and serve most on-site primary care applications. Notwithstanding the importance of research on this issue, midwives are only mentioned in the
Regulations on Midwifery / Paramedics and the Law of Ukraine “On increasing the availability and quality of health care in rural areas”, the main proposal of which is the introduction of telemedicine in rural medicine. Of Ukraine. Although the Law of Ukraine “On State Financial Guarantees of Public Health Services” also left its mark on rural health centers, including negative ones.
Therefore, it is necessary to analyze the situation of the medical midwifery points before and after the medical reform, identify the shortcomings for the medical centers, and find ways to solve them.
Keywords: paramedics, medical reform, primary care reform, medical center, rural medicine.
Fertile Intersectional F1 Hybrids of 4x Vaccinium meridionale (Section Pyxothamnus) and Highbush Blueberry, V. corymbosum (Section Cyanococcus)
Authors: Ehlenfeldt Mark K. , Luteyn James L.
Published: January 2021

Vaccinium meridionale (section Pyxothamnus), a tetraploid species native to higher altitude locations in Jamaica, Colombia, and Venezuela, is of considerable interest to blueberry breeders for its profuse, concentrated flowering and monopodial plant structure, both of which may be useful in breeding for mechanical harvest. In this study, tetraploid V. meridionale was successfully hybridized as a male with 4x V. corymbosum (section Cyanococcus, highbush blueberry). The first-generation hybrids with highbush blueberry selections were intermediate in morphology and notably vigorous. The 4x F1 hybrids displayed variable branching structure, dormancy, prolificacy, fruit wax, etc.; however, most appear to be deciduous to semi-evergreen, with small, dark-colored fruit. The F1 hybrids displayed good fertility as females in backcrosses to 4x highbush and these crosses have produced numerous offspring morphologically indistinguishable from 4x highbush at the seedling stage. Evaluations of male fertility found variation for pollen production and quality but, significantly, found some clones with very good shed, high stainability, and almost complete tetrad production. The fertility suggests that these hybrids, despite being derived from intersectional crosses, might be conventionally used without significant difficulty. These hybrids also have potential value for the nascent V. meridionale breeding efforts occurring in Colombia, South America.
Reduction of structural Fe(III) in nontronite by humic substances in the absence and presence of Shewanella putrefaciens and accompanying secondary mineralization
Authors: Zuo Hongyan , Huang Liuqin , Chu Rosalie K. , Tolic Nikola , Washton Nancy , Zhu Zihua , Edelmann Richard E. , Elagamy Samar , Sommer Andre , Luan Fubo , Zeng Qiang , Chen Yu , Hu Dafu , Zhan Di , Hu Jinglong , Dong Hailiang
Published: January 2021

Studies have shown the electron shuttling role of humic substances (HS) in enhancing microbial reduction of solid-phase Fe(III), but it is unknown if native HS can reduce structural Fe(III) in clays and how their chemical properties affect this process and secondary mineralization. The objective of this study was to evaluate the role of natural HS, Leonardite humic acid (LHA), and Pahokee Peat humic acid (PPHA) in reducing structural Fe(III) in nontronite with or without Shewanella putrefaciens. The extent of Fe(III) reduction was determined with a wet chemical method. Electrochemical methods, spectroscopy, and mass spectrometry were used to determine the changes of HS electrochemical and molecular composition after bioreduction. X-ray diffraction and electron microscopy were used to observe mineralogical transformations. The results showed that natural HS not only served as an electron donor to abiotically reduce Fe(III) in nontronite but also served as an electron shuttle to enhance Fe(III) bioreduction by S. putrefaciens. In the presence of CN32 cells, both the rate and extent of Fe(III) reduction significantly increased. Between the two HS, PPHA was more effective. The final bioreduction extents were 12.2 and 17.8% with LHA and PPHA, respectively, in bicarbonate buffer. Interestingly, when CN32 cells were present, LHA and PPHA donated more electrons to NAu-2, suggesting that CN32 cells were able to make additional electrons of LHA and PPHA available to reduce structural Fe(III). Although LHA reduced less Fe(III), it induced more extensive mineral transformation. In contrast, PPHA reduced more Fe(III) but did not induce any mineralogical change. These contrasting behaviors between the two humic acids are ascribed to their differences in electron-donating capacity, reactive functional group distribution, and metal complexation capacity. A unique set of secondary minerals, including talc, illite, silica, albite, ilmenite, and ferrihydrite formed as a result of reduction. The results highlight the importance of coupled C and Fe biogeochemical transformations and have implications for nutrient cycling and contaminant migration in the environment.
Xi Jinping, “China Dream”, and Chinese Military Diplomacy to ASEAN
Authors: Sinaga Lidya Christin
Published: January 2021

The rise of Xi Jinping has brought together the idea of the ‘China Dream’ as a great revival of China. Since the dream referred to the nationalism spirit of a ‘century of humiliation,’ it has made national security issues as the core of China’s diplomacy. While the national security-related foreign policy has enhanced the military's role in China’s foreign policy-making, it brings consequences for China’s tougher stance in protecting China’s national security. However, Xi Jinping’s notion of using military diplomacy has started uneasy relationships between China and some ASEAN countries resulting in ‘ongoing negotiation without progress’ for the South China Sea dispute. The research examines the impacts of the military’s growing role in China’s foreign policy under Xi Jinping to its military diplomacy in ASEAN. The results show that Xi Jinping’s leadership and vision of the China Dream, which uses military diplomacy as a key tool for advancing its whole diplomatic goals, has been seen as a sign of growing assertiveness.
PREPARATION OF HYBRID POLYMERS AND USE AS CONTROLLED RELEASE DELIVERY VEHICLE FOR VOLATILE REPELLENT TO CONTROL Aedes aegypti
Authors: Oliveira Ygor , Delfino Ângladis , Carvalho Cenira , Conserva Lucia , Abreu Fabiane
Published: January 2021

The Zika virus is a flavivirus spread by the Aedes aegypti mosquito, which also transmits dengue and chikungunya. Current forms of control include elimination of mosquito breeding sites, use of insecticides, repellents and other means. The present study developed new adsorbent materials based on silica and biomaterials capable of releasing an Aedes aegypti mosquito repellent. The materials synthesized were characterized by Fourier-Transform Infrared, thermogravimetric analysis, Scanning Electron Microscopy and Brunauer, Emmett and Teller analysis and tested with citronella, an essential oil widely recognized as a natural insect repellent. The materials presented type IV isotherms, with type H1 hysteresis, characteristic of mesoporous materials, with pore diameters of between 50 Å and 80 Å. Materials based on silica-chitosan-glutaraldehyde dried in an oven (SCGO) and silica-chitosan-glutaraldehyde dried under a vacuum (SCGV) were found to produce more controlled release of citronella essential oil. The bioassay test was performed using a horizontal double-choice olfactometer. The adsorbent SCGO remained stable for the four hours of the bioassay, while the commercial repellent became ineffective after two hours of release. The production of a prototype in the form of bracelets, stickers releasing a volatile repellent is therefore proposed as a method for the control of the mosquito in urban areas.