bluuebunny/crossref_metadata_2025 huggingface dataset:
Nephrons and non-relapse mortality: simplified comorbidity index and acute kidney injury are associated with NRM in adults undergoing allogeneic hematopoietic cell transplant
Authors: Robinson Clark Raymond , Habib Alma , Klomjit Nattawat , Cao Qing , Holtan Shernan Grace
Published: March 2024

The Simplified Comorbidity Index (SCI) is a recently published 5-component, pre-transplant tool to predict non-relapse mortality (NRM) in allogeneic hematopoietic cell transplantation (alloHCT) patients. The SCI captures chronic kidney disease (CKD) using estimated glomerular filtration rate (eGFR) based on the CKD-EPI equation (KDIGO 2021 CKD-EPI), which may be more sensitive to predict risk of NRM than the creatinine cut-off in the 16-component, Hematopoietic Cell Transplant—Comorbidity Index (HCT-CI). We retrospectively assessed the ability of the SCI to risk-stratify patients and the impact of acute kidney injury (AKI) to NRM in adults who underwent alloHCT at the University of Minnesota. We included 373 patients who underwent their first alloHCT between 2015 and 2019. Through multivariate analysis, we found that patients with an SCI of greater than 4 had a higher risk of NRM. Additionally, we noted that AKIs stages 2–3 prior to day +100 was independently associated with a 3-fold greater NRM than patients who did not experience clinically significant AKI.
Patients' experiences and perspectives regarding the use of digital technology to support exercise-based cardiac rehabilitation: a qualitative interview study
Authors: Zeller Anna , Gutenberg Johanna , Niebauer Josef , Crutzen Rik , Kulnik Stefan Tino
Published: March 2024

Introduction

Despite the well-known benefits of exercise-based cardiac rehabilitation for the secondary prevention of cardiovascular disease, participation in cardiac rehabilitation programmes and adherence to secondary prevention recommendations remain limited. Digital technologies have the potential to address low participation and adherence but attempts at implementing digital health interventions in real-life clinical practice frequently encounter various barriers. Studies about patients' experiences and perspectives regarding the use of digital technology can assist developers, researchers and clinicians in addressing or pre-empting patient-related barriers. This study was therefore conducted to investigate the experiences and perspectives of cardiac rehabilitation patients in Austria with regard to using digital technology for physical activity and exercise.

Methods

Twenty-five current and former cardiac rehabilitation patients (18 men and 7 women, age range 39 to 83) with various cardiac conditions were recruited from a clinical site in Salzburg, Austria. Semi-structured qualitative interviews were audio-recorded and transcribed verbatim. The analysis followed a descriptive phenomenological approach, applying the framework analysis method.

Results

The sample was diverse, including interviewees who readily used digital devices to support their physical activity, exercise and health monitoring, and interviewees who did not. Simplicity, convenience and accessibility were highlighted as important facilitators for the use of digital technology, while annoyance with digital devices, concerns about becoming dependent on them, or simply a preference to not use digital technology were commonly stated reasons for non-use. Interviewees' views on data protection, data sharing and artificial intelligence revealed wide variations in individuals' prior knowledge and experience about these topics, and a need for greater accessibility and transparency of data protection regulation and data sharing arrangements.

Discussion

These findings support the importance that is attributed to user-centred design methodologies in the conceptualisation and design of digital health interventions, and the imperative to develop solutions that are simple, accessible and that can be personalised according to the preferences and capabilities of the individual patient. Regarding data protection, data sharing and artificial intelligence, the findings indicate opportunity for information and education, as well as the need to offer patients transparency and accountability in order to build trust in digital technology and digital health interventions.
District-Wise Heterogeneity in Blood Pressure Measurements, Prehypertension, Raised Blood Pressure, and Their Determinants Among Indians: National Family Health Survey-5
Authors: Seenappa Kavyashree , Kulothungan Vaitheeswaran , Mohan Rohith , Mathur Prashant
Published: March 2024

Objective: The objective of the study was to determine the prevalence and determinants of ever-measured blood pressure, prehypertension, and raised blood pressure at national, state and district levels in India.

Methods: We analysed data from the National Family Health Survey (NFHS-5), on 743,067 adults aged 18–54 years. The sample consisted of 87.6% females and 12.4% males. We estimated prevalence rates and determined adjusted odds ratios for various dependent variables related to blood pressure. Geographical variations were visualized on the map of India, and multivariate logistic regression was employed at state and district levels, with significance set at p < 0.05.

Results: The prevalence of ever-measured blood pressure varied widely, from 30.3% to 98.5% across districts, with southern and northern regions showing higher rates. Prehypertension affected 33.7% of the population, with varying prevalence across districts. Raised blood pressure was there in 15.9%, with notably higher rates in southern region (16.8%). Determinants included age, gender, education, wealth, lifestyle, obesity, and blood glucose levels.

Conclusion: These findings demonstrate the subnational variations in blood pressure, can guide evidence-based interventions at the state and district level, towards reducing the burden of raised blood pressure and enhancing overall population health.
Extended State Kalman Filter-Based Model Predictive Control for Electro-Optical Tracking Systems with Disturbances: Design and Experimental Verification
Authors: Xia Wanrun , Mao Yao , Zhang Luyao , Guo Tong , Wang Haolin , Bao Qiliang
Published: March 2024

A modified Extended State Kalman Filter (ESKF)-based Model Predictive Control (MPC) algorithm is introduced to tailor the enhanced disturbance suppression in electro-optical tracking systems. Traditional control techniques, although robust, often struggle in scenarios with concurrent internal, external disturbances, and sensor noise. The proposed algorithm effectively overcomes these limitations by precisely estimating system states and actively mitigating disturbances, thus significantly boosting noise and perturbation control resilience. The primary contributions of this study include the integration of ESKF for accurate system state and disturbance estimation in noisy environments, the embedding of an ESKF estimation-compensation loop to simulate an improved disturbance-free system, and a simplified modeling approach for the controlled device. This designed structure minimizes the reliance on extensive system identification, easing the predictive control model-based constraints. Moreover, the approach incorporates total disturbance estimation into the optimization problem, safeguarding against actuator damage and ensuring high tracking accuracy. Through rigorous simulations and experiments, the ESKF-based MPC has demonstrated enhanced model error tolerance and superior disturbance suppression capabilities. Comparative analyses under varying model parameters and external disturbances highlight its exceptional trajectory tracking performance, even in the presence of model uncertainties and external noise.
Reaction Force-Based Position Sensing for Magnetic Levitation Platform with Exceptionally Large Hovering Distance
Authors: Bonetti Reto , Beglinger Lars , Mirić Spasoje , Bortis Dominik , Kolar Johann W.
Published: March 2024

This work introduces a novel sensing concept based on reaction forces for determining the position of the levitating magnet (mover) for magnetic levitation platforms (MLPs). Besides being effective in conventional magnetic bearings, the applied approach enables operation in systems where the mover is completely isolated from the actuating electromagnets (EMs) of the stator (e.g., located inside a sealed process chamber) while levitating at an extreme levitation height. To achieve active position control of the levitating mover by properly controlling the stator’s EM currents, it is necessary to employ a dynamic model of the complete MLP, including the reaction force sensor, and implement an observer that extracts the position from the force-dependent signals, given that the position is not directly tied to the measured forces. Furthermore, two possible controller implementations are discussed in detail: a basic PID controller and a more sophisticated state-space controller that can be chosen depending on the characteristics of the MLP and the accuracy of the employed sensing method. To show the effectiveness of the proposed position-sensing and control concept, a hardware demonstrator employing a 207 mm outer-diameter (characteristic dimension, CD) stator with permanent magnets, a set of electromagnets, and a commercial multi-axis force sensor is built, where a 0.36 kg mover is stably levitated at an extreme air gap of 104 mm.
Enhancing Sustainable Agriculture in China: A Meta-Analysis of the Impact of Straw and Manure on Crop Yield and Soil Fertility
Authors: Zhao Zhe , Yang Yali , Xie Hongtu , Zhang Yixin , He Hongbo , Zhang Xudong , Sun Shijun
Published: March 2024

As the main organic materials, straw and manure play a critical role in soil organic carbon (SOC) sequestration and crop yield in China. This meta-analysis evaluated the impact of straw and manure amendments, both individually and combined, on crop yield, SOC, and soil nutrients in China by collecting 173 studies. The findings of this study revealed that straw return and manure application increased crop yields by 14.4% and 70.4%, respectively, overall. Combined straw and manure application gained a better improvement effect than straw alone but was less effective than manure alone. Regarding the straw return results, rice straw and a 3000–6000 kg ha−1 returning quantity improved crop yield, SOC, available phosphorus (AP), available potassium (AK), and total nitrogen (TN) the most; regarding the straw return form, straw incorporated into soil and biochar increased crop yield and SOC more, respectively; and <5 years and ≥5 years of straw return treatment increased crop yield and TN more, respectively. Regarding manure application, pig and chicken manure increased crop yield and TN more, respectively; a 50–80% substitution ratio and 10–20 years of duration were best for improving crop yield, SOC, AP, AK, and TN. This study highlights the importance of optimal organic amendment through straw or manure applications to achieve a win–win between crop yield and soil fertility under the requirement of sustainable agriculture.
A Review of Machine Learning Techniques in Agroclimatic Studies
Authors: Tamayo-Vera Dania , Wang Xiuquan , Mesbah Morteza
Published: March 2024

The interplay of machine learning (ML) and deep learning (DL) within the agroclimatic domain is pivotal for addressing the multifaceted challenges posed by climate change on agriculture. This paper embarks on a systematic review to dissect the current utilization of ML and DL in agricultural research, with a pronounced emphasis on agroclimatic impacts and adaptation strategies. Our investigation reveals a dominant reliance on conventional ML models and uncovers a critical gap in the documentation of methodologies. This constrains the replicability, scalability, and adaptability of these technologies in agroclimatic research. In response to these challenges, we advocate for a strategic pivot toward Automated Machine Learning (AutoML) frameworks. AutoML not only simplifies and standardizes the model development process but also democratizes ML expertise, thereby catalyzing the advancement in agroclimatic research. The incorporation of AutoML stands to significantly enhance research scalability, adaptability, and overall performance, ushering in a new era of innovation in agricultural practices tailored to mitigate and adapt to climate change. This paper underscores the untapped potential of AutoML in revolutionizing agroclimatic research, propelling forward the development of sustainable and efficient agricultural solutions that are responsive to the evolving climate dynamics.
Seasonal Dynamics of Epigeic Arthropods under the Conditions of Ecological Management of the Triticum aestivum Crop
Authors: Langraf Vladimír , Petrovičová Kornélia
Published: March 2024

The policy of the European Union on land management promotes sustainable agriculture with an emphasis on the protection of biodiversity and the environment. Organic agriculture is the most appropriate alternative to ensure this common goal. The aim of this study was to determine the influence of factors such as pH, moisture, nitrogen potassium, phosphorus and grass herbaceous vegetation on the spatial structure of epigeic arthropods during the spring and summer seasons under organic farming conditions. Research took place between 2020 and 2022, and we recorded 14,988 individuals belonging to 16 taxa using pitfall traps. Between the years 2020 and 2022, we confirmed a decrease in the number of individuals and taxa of epigeic arthropods from the grass herbaceous vegetation to the interior of the field during the summer seasons. This decline was not confirmed in the spring seasons. Phosphorus, potassium, nitrogen, moisture and pH factors also had a significant influence on the spatial structure of epigeic arthropods. Our results show that the higher number of individuals and taxa at the grass herbaceous vegetation occurred only during the summer period. This fact contributes to an increase in biomass and, consequently, the yield of crops.
Drone-Based Multispectral Remote Sensing Inversion for Typical Crop Soil Moisture under Dry Farming Conditions
Authors: Qu Tengteng , Li Yaoyu , Zhao Qixin , Yin Yunzhen , Wang Yuzhi , Li Fuzhong , Zhang Wuping
Published: March 2024

Drone multispectral technology enables the real-time monitoring and analysis of soil moisture across vast agricultural lands. overcoming the time-consuming, labor-intensive, and spatial discontinuity constraints of traditional methods. This study establishes a rapid inversion model for deep soil moisture (0–200 cm) in dryland agriculture using data from drone-based multispectral remote sensing. Maize, millet, sorghum, and potatoes were selected for this study, with multispectral data, canopy leaf, and soil moisture content at various depths collected every 3 to 6 days. Vegetation indices highly correlated with crop canopy leaf moisture content (p < 0.01) and were identified using Pearson correlation analysis, leading to the development of linear and nonlinear regression models for predicting moisture content in canopy leaves and soil. The results show a significant linear correlation between the predicted and actual canopy leaf moisture levels for the four crops, according to the chosen vegetation indices. The use of canopy leaf moisture content to predict surface soil moisture (0–20 cm) demonstrated enhanced accuracy. The models designed for the top 20 cm of soil moisture successfully estimated deep soil moisture levels (up to 200 cm) for all four crops. The 20 cm range soil moisture model showed improvements over the 10 cm range model, with increases in Root Mean Square Error (RMSE), Mean Absolute Error (MAE), Coefficient of Determination (R2), and Nash–Sutcliffe Efficiency Coefficient (NSE) by 0.4, 0.8, 0.73, and 0.34, respectively, in the corn area; 0.28, 0.69, 0.48, and 0.25 in the millet area; 0.4, 0.48, 0.22, and 0.52 in the sorghum area; and 1.14, 0.81, 0.73, and 0.56 in the potato area, all with an average Relative Error (RE) of less than 10% across the crops. Using drone-based multispectral technology, this study forecasts leaf water content via vegetation index analysis, facilitating swift and effective soil moisture inversion. This research introduces a novel method for monitoring and managing agricultural water resources, providing a scientific basis for precision farming and moisture variation monitoring in dryland areas.
Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats
Authors: Lu Zeyu , Zhang Lingtian , Mu Qing , Liu Junyang , Chen Yu , Wang Haoyuan , Zhang Yanjun , Su Rui , Wang Ruijun , Wang Zhiying , Lv Qi , Liu Zhihong , Liu Jiasen , Li Yunhua , Zhao Yanhong
Published: March 2024

Due to recent innovations in gene editing technology, great progress has been made in livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other livestock. Gene-editing technology involves knocking in, knocking out, deleting, inhibiting, activating, or replacing specific bases of DNA or RNA sequences at the genome level for accurate modification, and such processes can edit genes at a fixed point without needing DNA templates. In recent years, although clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene-editing technology has been widely used in research into the genetic breeding of animals, the system’s efficiency at inserting foreign genes is not high enough, and there are certain off-target effects; thus, it is not appropriate for use in the genome editing of large livestock such as cashmere goats. In this study, the development status, associated challenges, application prospects, and future prospects of CRISPR/Cas9-mediated precision gene-editing technology for use in livestock breeding were reviewed to provide a theoretical reference for livestock gene function analysis, genetic improvement, and livestock breeding that account for characteristics of local economies.
Design and Experiment of Automatic Transport System for Planting Plate in Plant Factory
Authors: Jia Dongdong , Guo Wenzhong , Wang Lichun , Zheng Wengang , Gao Guohua
Published: March 2024

In the plant factories using stereoscopic cultivation systems, the cultivation plate transport equipment is an essential component of production. However, there are problems, such as high labor intensity, low levels of automation, and poor versatility of existing solutions, that can affect the efficiency of cultivation plate transport processes. To address these issues, this study designed a cultivation plate transport system that can automatically input and output cultivation plates, and can flexibly adjust its structure to accommodate different cultivation frame heights. We elucidated the working principles of the transport system and carried out structural design and parameter calculation for the lift cart, input actuator, and output actuator. In the input process, we used dynamic simulation technology to obtain an optimum propulsion speed of 0.3 m·s−1. In the output process, we used finite element numerical simulation technology to verify that the deformation of the cultivation plate and the maximum stress suffered by it could meet the operational requirements. Finally, operation and performance experiments showed that, under the condition of satisfying the allowable amount of positioning error in the horizontal and vertical directions, the horizontal operation speed was 0.2 m·s−1, the maximum positioning error was 2.87 mm, the vertical operation speed was 0.3 m·s−1, and the maximum positioning error was 1.34 mm. Accordingly, the success rate of the transport system was 92.5–96.0%, and the operational efficiency was 176–317 plates/h. These results proved that the transport system could meet the operational requirements and provide feasible solutions for the automation of plant factory transport equipment.
Analysis and Structural Optimization Test on the Collision Mechanical Model of Blade Jun-Cao Grinding Hammer
Authors: Zheng Shuhe , Chen Chongcheng , Guo Yuming
Published: March 2024

Aiming at the problems found in grinding Jun-Cao, such as poor grinding effect and high grinding power of mill, this study proposes a blade Jun-Cao grinding hammer based on the traditional hammer mill. With dynamics model analysis, it had better performance than a traditional hammer. By simulating the operation process in the DEM, forces on Jun-Cao and their motions were analyzed. By optimizing the structural parameters of the hammer blade based on multiobjective optimization using the genetic algorithm, an optimal solution set was obtained as a reference for practical production. Meanwhile, a bench test was designed to compare the traditional rectangular hammer with the new blade hammer regarding the operation effect. The result proved the following: (1) cutting edge length, cutting edge thickness and hammer thickness had a significant influence on the grinding effect and grinding power; (2) a total of 22 optimal solution sets were obtained, based on which the blade hammer with a cutting edge length of 45 mm, a cutting edge thickness of 3 mm and a hammer thickness of 7 mm was finally selected in the bench test; (3) the bench test proved that the blade hammer was generally superior to the traditional rectangular hammer with the output per kilowatt-hour having been improved by 13.55% on average.
Tobacco/Salvia miltiorrhiza Intercropping Improves Soil Quality and Increases Total Production Value
Authors: Su Xueqi , Guo Xiaomeng , Chen Qian , Sun Zheng , Shang Xianchao , Gao Yun , Yu Tao , Zhang Li , Yang Long , Hou Xin
Published: March 2024

Unreasonable cultivation methods and management measures have led to widespread obstacles in tobacco continuous cropping in planting areas, resulting in reduced tobacco yield and soil degradation. Therefore, intercropping tobacco with other crops is an effective strategy to improve continuous cropping barriers. In this study, flue-cured tobacco NC102 and conventional planting varieties of Salvia miltiorrhiza were used as materials, and four treatments of flue-cured tobacco monoculture (CK), flue-cured tobacco, and Salvia miltiorrhiza at a ratio of 1:1 (TS11), 2:2 (TS22), and 2:3 (TS23), respectively, were set up to study their effects on soil microorganisms, physical and chemical properties, and yield and quality of flue-cured tobacco. The results showed that intercropping Salvia miltiorrhiza increased the number of soil bacteria and actinomycetes, decreased the number of fungi, and increased the activity of urease and sucrase. The content of available nitrogen and available phosphorus in intercropping Salvia miltiorrhiza soil was significantly higher than that of the flue-cured tobacco monoculture, while the content of available potassium was lower than that of the flue-cured tobacco monoculture. The soil environment was more conducive to the growth of flue-cured tobacco. Compared with the flue-cured tobacco monoculture, the proportion of superior tobacco in intercropping Salvia miltiorrhiza increased by 2.2–3.4%, and the ratio of potassium to chlorine in leaves of different parts of flue-cured tobacco increased by 12.3–75.0%. The content of total sugar and soluble sugar in middle and upper leaves of intercropping flue-cured tobacco was higher than that of the flue-cured tobacco monoculture, which improved the quality of flue-cured tobacco. From the analysis of the chemical composition of tobacco leaves, TS11 (flue-cured tobacco and Salvia miltiorrhiza intercropping row ratio of 1:1) had the best treatment effect, potassium content, total sugar, reducing sugar content, and potassium chloride ratio of flue-cured tobacco were the highest, the chlorine content was the lowest, and the quality was better than other treatments. From the analysis of total output value, the total output value of TS22 (flue-cured tobacco and Salvia miltiorrhiza intercropping row ratio of 2:2) was higher than that of other intercropping treatments. In 2018 and 2019, the total output value increased by 21.3% and 22.4%, respectively, compared with the flue-cured tobacco monoculture. The intercropping advantage was obvious, and the treatment effect was the best.
Predation and Biocontrol Potential of Eupeodes corollae Fabricius (Diptera: Syrphidae) on Wheat Aphids
Authors: Jiang Shanshan , Li Hui , Wang Hainuo , Liu Xiaoxia , Wu Kongming
Published: March 2024

Wheat aphids are major pests of wheat and a significant threat to global food security. Eupeodes corollae Fabricius is one of the dominant species of wheat field hoverflies, but its ability and role in wheat aphid control lack systematic research. This study on the predatory function responses of E. corollae to Rhopalosiphum padi Linnaeus, Schizaphis graminum Rondani, and Sitobion miscanthi, Takahashi showed that the maximum daily predation (1/Th) of 2nd instar E. corollae larvae was 166.67, 125.00, and 142.86, and that of 3rd instar larvae was 333.33, 250.00, and 250.00, respectively. The cage simulation test indicated that the wheat aphid population decline rate was 100% at the 60th hour of inoculation of 3rd instar E. corollae larvae at a 1:100 ratio. Eupeodes corollae exhibited a predatory relationship with all three wheat aphid species in the wheat fields of Hebei Province, China, and the corrected predation detection rates of E. corollae larvae against R. padi, S. graminum, and S. miscanthi were 12.36%, 1.08%, and 28.77% in 2022, and 6.74%, 0.82%, and 37.56% in 2023, respectively. The results of this study clarify the predatory ability of E. corollae on wheat aphids and the predatory relationship between them and provide technical support for the management of wheat aphids using the bio-control ecological service function of E. corollae.
Early Detection of Rice Leaf Blast Disease Using Unmanned Aerial Vehicle Remote Sensing: A Novel Approach Integrating a New Spectral Vegetation Index and Machine Learning
Authors: Zhao Dongxue , Cao Yingli , Li Jinpeng , Cao Qiang , Li Jinxuan , Guo Fuxu , Feng Shuai , Xu Tongyu
Published: March 2024

Leaf blast is recognized as one of the most devastating diseases affecting rice production in the world, seriously threatening rice yield. Therefore, early detection of leaf blast is extremely important to limit the spread and propagation of the disease. In this study, a leaf blast-specific spectral vegetation index RBVI = 9.78R816−R724 − 2.08(ρ736/R724) was designed to qualitatively detect the level of leaf blast disease in the canopy of a field and to improve the accuracy of early detection of leaf blast by remote sensing by unmanned aerial vehicle. Stacking integrated learning, AdaBoost, and SVM were used to compare and analyze the performance of the RBVI and traditional vegetation index for early detection of leaf blast. The results showed that the stacking model constructed based on the RBVI spectral index had the highest detection accuracy (OA: 95.9%, Kappa: 93.8%). Compared to stacking, the detection accuracy of the SVM and AdaBoost models constructed based on the RBVI is slightly degraded. Compared with conventional SVIs, the RBVI had higher accuracy in its ability to qualitatively detect leaf blast in the field. The leaf blast-specific spectral index RBVI proposed in this study can more effectively improve the accuracy of UAV remote sensing for early detection of rice leaf blast in the field and make up for the shortcomings of UAV hyperspectral detection, which is susceptible to interference by environmental factors. The results of this study can provide a simple and effective method for field management and timely control of the disease.
Influence of Nutrient Solutions in an NGS® Soilless System on the Yield, Quality and Shelf Life of Fresh-Cut Commercial Mint at Different Harvest Times
Authors: Hazrati Saeid , Pignata Giuseppe , Casale Manuela , Hosseini Seyyed Jaber , Nicola Silvana
Published: March 2024

The optimal fertilizer concentration for Mentha plants is contingent on the growing systems and harvest time, serving as operational solutions to control and enhance quality and yield. This study aimed to determine the effects of three macronutrients concentration in hydroponic nutrient solution (HNS) during three harvest times on the growth, quality, yield, and shelf life of three mint species (M. spicata L. var. viridis (MV); M. piperita L. (MP); M. spicata L. var. rubra (MR)) grown in a New Growing System (NGS®). Total dry matter (DM), nitrate (NO3−), phosphate (PO43−), and calcium carbonate (CaCO3) concentrations were increased with the addition of higher levels of nutrient fertilization in three species. When the ion concentration of the HNS was increased, total fresh yield decreased. The highest total bacterial count (TBC) was obtained in MR species in the three harvests in all the levels of HNS. The lowest browning potential (BP) and soluble o-quinone (So-Q) levels were observed at second harvest in the MR species with the application of one of the two HNS high in nitrogen (N). In conclusion, the combination of optimal HNS ion concentration and appropriate species is considered essential to obtain suitable yield, quality, and ensure shelf life of mint.
Morphometric and Enzymatic Changes in Gills of Rainbow Trout after Exposure to Elevated Temperature—Indications for Gill Remodeling
Authors: Lahnsteiner Franz
Published: March 2024

Seven-month-old rainbow trout acclimated to 9 °C were used. The fish were gradually adapted to a water temperature of 20 °C over a period of seven days and then exposed to this temperature for 32 days. Changes in gill morphometry and histology and in enzyme activities in comparison to fish kept at 9 °C were investigated. No histopathological abnormalities were discerned at the heightened temperature. The gill epithelium thickened by approximately 40%, suggesting an increase in the branchial diffusion barrier for ions, water, and gases. Concurrently, there was a significant decrease in the activities of gill H+-ATPase and Na+/K+-ATPase, indicative of a reduction in osmoregulation under elevated temperatures. Carbonic anhydrase activity exhibited an increase following the 32-day exposure to 20 °C, potentially mitigating the adverse effects of increased gill epithelium thickness on gaseous exchange. There were no indications of gill surface enlargement as the measurements of the length of the primary and secondary lamellae, as well as of the distances between them, were similar at 9 and 20 °C. The activities of the gill enzymes associated with glycolysis and the citric acid cycle displayed a varied response following the 32-day exposure of rainbow trout to 20 °C. Pyruvate kinase decreased, while lactate dehydrogenase increased, and malate dehydrogenase remained constant. This might suggest a decrease in the glycolytic rate, a greater reliance on anaerobic pathways at 20 °C compared to 9 °C, and the consistent efficiency of the citric acid cycle in the gills of rainbow trout in response to elevated temperatures. In summation, the data suggest a remodeling of rainbow trout gills in response to elevated temperatures, affecting both morphometric and metabolic aspects.
Shark Dive and Hologram Zoo: Two Case Studies of Virtual Animal Encounters as Possible Models for Sustainable Wildlife Tourism
Authors: Scollen Rebecca , Mason Andrew
Published: March 2024

Wildlife tourism is commonly argued to positively contribute to nature conservation by providing humans with educational and meaningful first-hand experiences with animals. However, concern for the sustainability of nature due to the negative impacts of wildlife tourism on animals and the environment invites alternative virtual opportunities for close encounters. Participant Observation framed by an Animal Studies perspective is employed to interpret two contemporary Australian family entertainments, Shark Dive and Hologram Zoo, centred on providing people with wild animal interactions but without real animals present. Shark Dive is a theatrical puppetry production replicating a real shark dive, and Hologram Zoo is an augmented reality experience that displays a variety of animals in 3D. The content of both is examined to understand how the animals are imagined, experienced, and given significance to determine whether they offer viable alternatives or complementary additions to in situ wildlife tourism. Findings reveal both hold potential for virtual wildlife tourism. Shark Dive provides a positive representation of marine life, generating awareness about conservation and encouraging self-reflection. Hologram Zoo’s depiction of wild animals is impressive, but to more closely align with contemporary wildlife tourism ideals, the experience could enhance its conservation messaging and positive human–animal interactions.
Zoofolkloristics: Imagination as a Critical Component
Authors: Brooks Pribac Teya , Golež Kaučič Marjetka
Published: March 2024

Nonhuman animal protagonists of folklore texts in the European space have tended to be perceived primarily as performing a symbolic and metaphoric function. But behind the symbols and the metaphors hide real flesh-and-blood nonhuman animals, and flesh-and-blood humans interacting with them, mostly from a position of power. The emerging discipline of zoofolkloristics considers nonhuman animals in their own right. Through critical analysis of folklore material, zoofolkloristics examines the role of animals and power relations within the interspecies entanglement with the aim of deconstructing the oppressive system and establishing multispecies justice. We begin this paper with a brief reflection on the ‘historical animal’ as an embodied being and a human construct. We then perform a critical re-reading of three animal-related folklore texts from the Slovenian tradition and, applying Hubert Zapf’s concept of imaginative counter-discourse, consider the potential of imagination as a methodological tool in the transformative program of zoofolkloristics. Implications for animal ethics, liberation, and conservation are also discussed.
The Clinical Efficacy of Adding Ceftazidime/Avibactam to Standard Therapy in Treating Infections Caused by Carbapenem-Resistant Klebsiella pneumonia with blaOXA-48-like Genes
Authors: Abu Jaber Al Maamon R. , Basgut Bilgen , Hawan Ali Abdullah , Al Shehri Ali Amer , AlKahtani Sultan Ahmad , Ahmed Nehad J. , Abdi Abdikarim
Published: March 2024

Ceftazidime/avibactam (CAZ-AVI) is FDA-approved for managing infections caused by resistant gram-negative bacilli, particularly infections via carbapenem-resistant Enterobacterales pathogens. The clinical data are still limited, particularly those in Saudi Arabia. The present study is a retrospective cohort study that was carried out at the Armed Forces Hospital in the southern region of Saudi Arabia to compare the clinical and microbiological outcomes for CAZ-AVI-treated patients as monotherapy and as an add-on to standard therapy for carbapenem-resistant Klebsiella pneumonia (CRKP) OXA-48 infections to those treated with standard drugs. The study included CRKP OXA-48-like infected patients who were administered antibiotics for more than seven days from 1 August 2018 to May 2023. Patients’ baseline characteristics and demography were extracted from the clinical records, and their clinical/microbiology efficiencies were assessed as per the corresponding definitions. Univariate and multivariate logistic regressions were conducted to identify the potential independent variable for CAZ-AVI efficiency. A total of 114 patient files were included for the evaluation. Among these patients, 64 used CAZ-AVI combined with standard therapy and were included in the intervention group, and 50 of them used standard therapy and were included in the comparative group. Following analysis, CAZ-AVI’s clinical success was 42.2% (p = 0.028), while the intervention versus comparative groups showed decreased 30-day all-cause mortality (50.0% versus 70.0%; p = 0.036) and infection recurrence (7.8% versus 24.0%; p = 0.019), as well as substantially increased rates of microbial eradication (68.8% versus 42.0%; p = 0.007). CAZ-AVI add-on therapy rather than monotherapy showed statistically significant favored clinical and microbial outcomes over the standard therapy. Furthermore, sex (female %), ICU admission, and fever were negatively associated with patients’ 30-day all-cause mortality, serving as independent negative factors. Only fever, CRP bio levels, inotropes, and ICU admissions were significant predictors influencing the CAZ-AVI’s clinical efficiency. The duration of CAZ-AVI therapy positively influenced CAZ-AVI’s microbial eradication, while both WBC counts and fever experiences were negative predictors. This study shows the effective usage of CAZ-AVI against CRKP OXA-48-like infections. The influencing independent variables depicted here should recommend that clinicians individualize the CAZ-AVI dose based on co-existing risk factors to achieve optimal survival and efficacy. Prospective multicenter and randomized control studies are recommended, with individualized CAZ-AVI precision administration implemented based on patients’ characteristics.
Helicobacter pylori in Inflammatory Bowel Diseases: Active Protagonist or Innocent Bystander?
Authors: Bretto Elisabetta , Frara Simone , Armandi Angelo , Caviglia Gian Paolo , Saracco Giorgio Maria , Bugianesi Elisabetta , Pitoni Demis , Ribaldone Davide Giuseppe
Published: March 2024

Helicobacter pylori (H. pylori) infection is a prominent entity within human infectious diseases which cause chronic gastritis, peptic ulcers, gastric malignancies, and extragastric disorders. Its persistent colonization can lead to a systemic inflammatory cascade, potentially instigating autoimmune responses and contributing to the pathogenesis of autoimmune diseases. While the specific etiopathogenesis of inflammatory bowel diseases (IBDs) is still unknown, it is widely recognized that immunological, genetic, and environmental factors are implicated. Various bacterial and viral pathogens have been implicated in the pathogenesis of IBDs. Numerous studies suggest a correlation between H. pylori infection and IBDs. While subject to debate, this link suggests that the bacterium’s presence somehow impacts the progression of IBDs by modifying the diversity of the gut microbiota, consequently altering local chemical profiles and disrupting the pattern of gut immune response. However, epidemiological evidence indicates a protective role of H. pylori infection against the onset of autoimmune diseases. Additionally, laboratory findings demonstrate H. pylori’s capacity to promote immune tolerance and restrict inflammatory reactions. The aim of this review is to elucidate the proposed mechanisms and confounding factors that underlie the potential association between H. pylori infection and IBDs.
A Single Dose of Piperacillin Plus Tazobactam Gel as an Adjunct to Professional Mechanical Plaque Removal (PMPR) in Patients with Peri-Implant Mucositis: A 6-Month Double-Blind Randomized Clinical Trial
Authors: Ilyes Ioana , Boariu Marius , Rusu Darian , Iorio-Siciliano Vincenzo , Vela Octavia , Boia Simina , Kardaras Georgios , Șurlin Petra , Calniceanu Horia , Jentsch Holger , Lodin Alexandru , Stratul Stefan-Ioan
Published: March 2024

Objectives: This randomized, placebo-controlled, double-masked clinical trial aimed to evaluate the clinical and microbiological efficacy of professional mechanical plaque removal (PMPR) with or without adjunctive application of piperacillin plus tazobactam gel in the treatment of peri-implant mucositis (PiM) for up to 6 months. Materials and Methods: The study included 31 patients with peri-implant mucositis (bleeding on probing (BoP) > 1 at at least one site at baseline, absence of peri-implant bone loss compared with a previous radiograph). After randomized assignment to test and control groups, patients received full-mouth supragingival scaling with or without piperacillin plus tazobactam gel. Clinical examination was performed at baseline and after 3 and 6 months, and a microbiological examination was performed at baseline and after 3 months. Results: After six months, both treatment modalities resulted in significant reductions and improvements in clinical parameters at the implant sites. Neither study group achieved a complete resolution of PiM (i.e., BoP ≤ 1 per implant). The number of implants with BoP decreased statistically significantly between subsequent time points (p < 0.001) in both the test and the control group. Significant BoP differences (p = 0.039) were observed between groups at 6 months (difference to baseline) following therapy. Conclusions: Within the limitations of the present study, the single use of a slow-release, locally applied antibiotic combination of piperacillin and tazobactam gel, adjunctive to PMPR, showed an improvement in clinical variable of implants diagnosed with PiM. The adjunctive treatment resulted in higher BoP reduction when compared to the control, but no significant differences were observed regarding the changes in other clinical and microbiological parameters.
Scientific Rationale and Clinical Basis for Clindamycin Use in the Treatment of Dermatologic Disease
Authors: Armillei Maria K. , Lomakin Ivan B. , Del Rosso James Q. , Grada Ayman , Bunick Christopher G.
Published: March 2024

Clindamycin is a highly effective antibiotic of the lincosamide class. It has been widely used for decades to treat a range of skin and soft tissue infections in dermatology and medicine. Clindamycin is commonly prescribed for acne vulgaris, with current practice standards utilizing fixed-combination topicals containing clindamycin that prevent Cutibacterium acnes growth and reduce inflammation associated with acne lesion formation. Certain clinical presentations of folliculitis, rosacea, staphylococcal infections, and hidradenitis suppurativa are also responsive to clindamycin, demonstrating its suitability and versatility as a treatment option. This review describes the use of clindamycin in dermatological practice, the mechanism of protein synthesis inhibition by clindamycin at the level of the bacterial ribosome, and clindamycin’s anti-inflammatory properties with a focus on its ability to ameliorate inflammation in acne. A comparison of the dermatologic indications for similarly utilized antibiotics, like the tetracycline class antibiotics, is also presented. Finally, this review addresses both the trends and mechanisms for clindamycin and antibiotic resistance, as well as the current clinical evidence in support of the continued, targeted use of clindamycin in dermatology.
Antimicrobial Action Mechanisms of Natural Compounds Isolated from Endophytic Microorganisms
Authors: Eshboev Farkhod , Mamadalieva Nilufar , Nazarov Pavel , Hussain Hidayat , Katanaev Vladimir , Egamberdieva Dilfuza , Azimova Shakhnoz
Published: March 2024

Infectious diseases are a significant challenge to global healthcare, especially in the face of increasing antibiotic resistance. This urgent issue requires the continuous exploration and development of new antimicrobial drugs. In this regard, the secondary metabolites derived from endophytic microorganisms stand out as promising sources for finding antimicrobials. Endophytic microorganisms, residing within the internal tissues of plants, have demonstrated the capacity to produce diverse bioactive compounds with substantial pharmacological potential. Therefore, numerous new antimicrobial compounds have been isolated from endophytes, particularly from endophytic fungi and actinomycetes. However, only a limited number of these compounds have been subjected to comprehensive studies regarding their mechanisms of action against bacterial cells. Furthermore, the investigation of their effects on antibiotic-resistant bacteria and the identification of biosynthetic gene clusters responsible for synthesizing these secondary metabolites have been conducted for only a subset of these promising compounds. Through a comprehensive analysis of current research findings, this review describes the mechanisms of action of antimicrobial drugs and secondary metabolites isolated from endophytes, antibacterial activities of the natural compounds derived from endophytes against antibiotic-resistant bacteria, and biosynthetic gene clusters of endophytic fungi responsible for the synthesis of bioactive secondary metabolites.
Selenomethionine Alleviates Deoxynivalenol-Induced Oxidative Injury in Porcine Intestinal Epithelial Cells Independent of MAPK Pathway Regulation
Authors: Huang Zhouyin , Zhong Haopeng , Li Ting , Wang Zirui , Chen Xingping , Zou Tiande , You Jinming , Chen Jun
Published: March 2024

Deoxynivalenol (DON) is a prevalent contaminant in feed and food, posing a serious threat to the health of both humans and animals. The pig stands as an ideal subject for the study of DON due to its recognition as the most susceptible animal to DON. In this study, the IPEC-J2 cells were utilized as an in vitro model to explore the potential of SeMet in alleviating the intestinal toxicity and oxidative injury in intestinal epithelial cells when exposed to DON. Cells were treated either with or without 4.0 μM SeMet, in combination with or without a simultaneous treatment with 0.5 μg/mL DON, for a duration of 24 h. Then, cells or related samples were analyzed for cell proliferation, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) level, gene expressions, and protein expressions. The results showed that SeMet mitigated the cellular toxicity caused by DON, evidenced by elevated cell proliferation and the reduced LDH release of IPEC-J2 cells in the SeMet + DON group vs. the DON group. Moreover, the SeMet treatment markedly promoted antioxidant functions and decreased the oxidative injury in IPEC-J2 cell, which is indicated by the decreased ROS level and up-regulated mRNA levels of GPX1, TXNRD1, Nrf2, and GCLC in IPEC-J2 cells in the SeMet + DON group vs. the DON group. However, in both the absence and presence of exposure to DON, the SeMet treatment did not affect the protein expression of MAPK (JNK, Erk1/2, and P38) and phosphorylated MAPK (p-JNK, p-Erk1/2, and p-P38) in IPEC-J2 cells. Collectively, SeMet alleviated the DON-induced oxidative injury in porcine intestinal epithelial cells independent of the MAPK pathway regulation.
Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement
Authors: Sip Szymon , Sip Anna , Szulc Piotr , Selwet Marek , Żarowski Marcin , Czerny Bogusław , Cielecka-Piontek Judyta
Published: March 2024

This study investigates the potential of formulated systems utilising haskap berry leaf extracts and dextran as carriers, to modulate both antioxidant and enzymatic inhibitory activities and their impact on the growth of specific bacterial strains. The analysis of antioxidant capacity, assessed through ABTS, CUPRAC, DPPH, and FRAP assays, revealed varying but consistently high levels across extracts, with Extract 3 (loganic acid: 2.974 mg/g, chlorogenic acid: 1.125 mg/g, caffeic acid: 0.083 mg/g, rutin: 1.137 mg/g, and quercetin: 1.501 mg/g) exhibiting the highest values (ABTS: 0.2447 mg/mL, CUPRAC: 0.3121 mg/mL, DPPH: 0.21001 mg/mL, and FRAP: 0.3411 mg/mL). Subsequent enzymatic inhibition assays demonstrated a notable inhibitory potential against α-glucosidase (1.4915 mg/mL, expressed as acarbose equivalent), hyaluronidase (0.2982 mg/mL, expressed as quercetin equivalent), and lipase (5.8715 µg/mL, expressed as orlistat equivalent). Further system development involved integration with dextran, showcasing their preserved bioactive compound content and emphasising their stability and potential bioactivity. Evaluation of the dextran systems’ impact on bacterial growth revealed a significant proliferation of beneficial strains, particularly the Bifidobacterium and lactobacilli genus (Bifidobacterium longum: 9.54 × 107 to 1.57 × 1010 CFU/mL and Ligilactobacillus salivarius: 1.36 × 109 to 1.62 × 1010 CFU/mL), suggesting their potential to modulate gut microbiota. These findings offer a foundation for exploring the therapeutic applications of haskap berry-based dextran systems in managing conditions like diabetes, emphasising the interconnected roles of antioxidant-rich botanical extracts and dextran formulations in promoting overall metabolic health.