bluuebunny/crossref_metadata_2025 huggingface dataset:
Loop Diuretic Administration in Patients with Acute Heart Failure and Reduced Systolic Function: Effects of Different Intravenous Diuretic Doses and Diuretic Response Measurements
Authors: Ruocco Gaetano , Feola Mauro , Nuti Ranuccio , Luschi Lorenzo , Evangelista Isabella , Palazzuoli Alberto
Published: November 2019

Background: Despite the fact that loop diuretics are a landmark in acute heart failure (AHF) treatment, few trials exist that evaluate whether the duration and timing of their administration and drug amount affect outcome. In this study, we sought to evaluate different loop diuretic infusion doses in relation to outcome and to diuretic response (DR), which was serially measured during hospitalization. Methods: This is a post-hoc analysis of a DIUR-HF trial. We divided our sample on the basis of intravenous diuretic dose during hospitalization. Patients taking less than 125 mg of intravenous furosemide (median value) were included in the low dose group (LD), patients with a diuretic amount above this threshold were inserted in the high dose group (HD). The DR formula was defined as weight loss/40 mg daily of furosemide and it was measured during the first 24 h, 72 h, and over the whole infusion period. Outcome was considered as death due to cardiovascular causes or heart failure hospitalization. Results: One hundred and twenty-one AHF patients with reduced ejection fractions (EF) were evaluated. The cardiovascular (CV) death/heart failure (HF) re-hospitalization rate was significantly higher in the HD group compared to the LD group (75% vs. 22%; p < 0.001). Both low DR, measured during the entire infusion period (HR 3.25 (CI: 1.92–5.50); p < 0.001) and the intravenous diuretic HD (HR 5.43 [CI: 2.82–10.45]; p < 0.001) were related to outcome occurrence. Multivariable analysis showed that DR (HR 3.01 (1.36–6.65); p = 0.006), intravenous diuretic HD (HR 2.83 (1.24–6.42); p=0.01) and worsening renal function (WRF) (HR 2.21 (1.14–4.28); p = 0.01) were related to poor prognosis. Conclusions: HD intravenous loop diuretic administration is associated with poor prognosis and less DR. Low DR measured during the whole intravenous administration better predicts outcome compared to DR measured in the early phases. ClinicalTrials.gov Acronym and Identifier Number: DIUR-HF; NCT01441245; registered on 23 September 2011.
Analysis of Perioperative Platelet Indices and Their Prognostic Value in Head and Neck Cancer Patients Treated with Surgery and Postoperative Radiotherapy: A Retrospective Cohort Study
Authors: Jank Bernhard J. , Haas Markus , Dunkler Daniela , Campion Nicholas J. , Brkic Faris F. , Heiduschka Gregor , Kadletz Lorenz
Published: November 2019

Objectives: Activated platelets might play an important role in tumor progression. Mean platelet volume (MPV) has been used as a surrogate marker for platelet activation, and therefore its value as a marker of tumor prognosis has attracted recent attention. In this study, we aimed to critically evaluate the prognostic significance of the perioperative platelet count (COP), MPV and the MPV/COP ratio in head and neck cancer patients. Additionally, we explored the individual postoperative trajectory of these indices and their association with overall survival (OS) and disease-free survival (DFS). Methods: We retrospectively evaluated 122 head and neck squamous cell carcinoma patients receiving surgery with curative intent followed by postoperative radiotherapy. Platelet indices were measured preoperatively and on days 1 and 7 postoperatively. OS and DFS were analyzed using Kaplan–Meier estimators, the log-rank test and uni and multivariable Cox models. Cutoffs to dichotomize patients for Kaplan–Meier curves and log-rank tests were empirically chosen at the respective median. The median follow-up was 8.8 years. Results: The adjusted preoperative COP, MPV and MPV/COP ratio were not associated with disease outcome. A low postoperative COP and a high MPV/COP ratio on the first postoperative day were independently associated with worse OS and DFS. In comparison to the preoperative measurements, patients whose COP increased by day 1 post-op showed a better OS (hazard ratio (HR) per 50 G/L increase: 0.73, 95% confidence interval (CI): 0.58–0.93, p = 0.013) and DFS (HR per 50 G/L increase: 0.74, 95% CI: 0.58–0.94, p = 0.018) in multivariable analysis. Conclusions: Our results suggest that a low postoperative COP and a high MPV/COP ratio represent a negative prognostic factor for OS and DFS. Notably, patients with an increase in COP by day 1 post-op when compared to their preoperative value showed a significantly better OS and DFS.
Identification of Risk of QT Prolongation by Pharmacists When Conducting Medication Reviews in Residential Aged Care Settings: A Missed Opportunity?
Authors: Christensen Louise , Turner J. Rick , Peterson Gregory M. , Naunton Mark , Thomas Jackson , Yee Kwang Choon , Kosari Sam
Published: November 2019

QT interval prolongation is associated with torsade de pointes and sudden cardiac death. QT prolongation can be caused by many drugs that are commonly prescribed in elderly residential aged care populations. The aim of this study was to investigate the prevalence of use of QT-prolonging drugs and to identify interventions made by pharmacists to reduce the risk of QT prolongation when conducting medication reviews in aged care. A retrospective analysis of 400 medication reviews undertaken by Australian pharmacists in aged care settings was conducted. The assessment included the risk of QT prolongation due to prescribed medications and other risk factors and the recommendations made by pharmacists to reduce the risk of QT prolongation. There was a high prevalence of the use of QT-prolonging medication, with 23% of residents (92 out of 400) taking at least one medication with a known risk of QT prolongation. Amongst the 945 prescribed drugs with any risk of QT prolongation, antipsychotics were the most common (n = 246, 26%), followed by antidepressants (19%) and proton pump inhibitors (13%). There appeared to be low awareness amongst the pharmacists regarding the risk of QT prolongation with drugs. Out of 400 reviews, 66 residents were categorised as high risk and were taking at least one medication associated with QT prolongation; yet pharmacists intervened in only six instances (9%), mostly when two QT-prolonging medications were prescribed. There is a need to increase awareness amongst pharmacists conducting medication reviews regarding the risk factors associated with QT prolongation, and further education is generally needed in this area.
Ample Spectrum Contractions and Related Fixed Point Theorems
Authors: Roldán López de Hierro Antonio Francisco , Shahzad Naseer
Published: November 2019

Simulation functions were introduced by Khojasteh et al. as a method to extend several classes of fixed point theorems by a simple condition. After that, many researchers have amplified the knowledge of such kind of contractions in several ways. R-functions, ( R , S ) -contractions and ( A , S ) -contractions can be considered as approaches in this direction. A common characteristic of the previous kind of contractive maps is the fact that they are defined by a strict inequality. In this manuscript, we show the advantages of replacing such inequality with a weaker one, involving a family of more general auxiliary functions. As a consequence of our study, we show that not only the above-commented contractions are particular cases, but also another classes of contractive maps correspond to this new point of view.
Modified Halpern Iterative Method for Solving Hierarchical Problem and Split Combination of Variational Inclusion Problem in Hilbert Space
Authors: Chaloemyotphong Bunyawee , Kangtunyakarn Atid
Published: November 2019

The purpose of this paper is to introduce the split combination of variational inclusion problem which combines the concept of the modified variational inclusion problem introduced by Khuangsatung and Kangtunyakarn and the split variational inclusion problem introduced by Moudafi. Using a modified Halpern iterative method, we prove the strong convergence theorem for finding a common solution for the hierarchical fixed point problem and the split combination of variational inclusion problem. The result presented in this paper demonstrates the corresponding result for the split zero point problem and the split combination of variation inequality problem. Moreover, we discuss a numerical example for supporting our result and the numerical example shows that our result is not true if some conditions fail.
Bipolar Fuzzy Relations
Authors: Lee Jeong-Gon , Hur Kul
Published: November 2019

We introduce the concepts of a bipolar fuzzy reflexive, symmetric, and transitive relation. We study bipolar fuzzy analogues of many results concerning relationships between ordinary reflexive, symmetric, and transitive relations. Next, we define the concepts of a bipolar fuzzy equivalence class and a bipolar fuzzy partition, and we prove that the set of all bipolar fuzzy equivalence classes is a bipolar fuzzy partition and that the bipolar fuzzy equivalence relation is induced by a bipolar fuzzy partition. Finally, we define an ( a , b ) -level set of a bipolar fuzzy relation and investigate some relationships between bipolar fuzzy relations and their ( a , b ) -level sets.
Image Interpolation Using a Rational Bi-Cubic Ball
Authors: Zulkifli Nur Atiqah Binti , Karim Samsul Ariffin Abdul , Shafie A’fza Binti , Sarfraz Muhammad , Ghaffar Abdul , Nisar Kottakkaran Sooppy
Published: November 2019

This study deals with the application of new rational bi-cubic Ball function with six parameters in image interpolation, especially for the grayscale image. These six free parameters can be modified to get better and quality image resolution, and refine the shape of the interpolating surface. This bivariate rational Ball function has been extended from univariate cases by using a tensor product approach. The proposed scheme is tested for image upscaling with factors of two and four through an efficient algorithm. The effectiveness of the proposed scheme is measured by using an image quality assessment (IQA), such as peak-signal-to-noise-ratio (PSNR), root mean square error (RMSE) or feature similarity (FSIM) index. Numerical and graphical results with comparisons against some existing scheme are presented by using MATLAB. The proposed scheme resulted in higher PSNR and FSIM, and smaller RMSE. Thus, the new rational bi-cubic Ball with six parameters is better than the existing scheme via an efficient algorithm.
Neutrosophic Portfolios of Financial Assets. Minimizing the Risk of Neutrosophic Portfolios
Authors: Boloș Marcel-Ioan , Bradea Ioana-Alexandra , Delcea Camelia
Published: November 2019

This paper studies the problem of neutrosophic portfolios of financial assets as part of the modern portfolio theory. Neutrosophic portfolios comprise those categories of portfolios made up of financial assets for which the neutrosophic return, risk and covariance can be determined and which provide concomitant information regarding the probability of achieving the neutrosophic return, both at each financial asset and portfolio level and also information on the probability of manifestation of the neutrosophic risk. Neutrosophic portfolios are characterized by two fundamental performance indicators, namely: the neutrosophic portfolio return and the neutrosophic portfolio risk. Neutrosophic portfolio return is dependent on the weight of the financial assets in the total value of the portfolio but also on the specific neutrosophic return of each financial asset category that enters into the portfolio structure. The neutrosophic portfolio risk is dependent on the weight of the financial assets that enter the portfolio structure but also on the individual risk of each financial asset. Within this scientific paper was studied the minimum neutrosophic risk at the portfolio level, respectively, to establish what should be the weight that the financial assets must hold in the total value of the portfolio so that the risk is minimum. These financial assets weights, after calculations, were found to be dependent on the individual risk of each financial asset but also on the covariance between two financial assets that enter into the portfolio structure. The problem of the minimum risk that characterizes the neutrosophic portfolios is of interest for the financial market investors. Thus, the neutrosophic portfolios provide complete information about the probabilities of achieving the neutrosophic portfolio return but also of risk manifestation probability. In this context, the innovative character of the paper is determined by the use of the neutrosophic triangular fuzzy numbers and by the specific concepts of financial assets, in order to substantiating the decisions on the financial markets.
New Quantum Estimates of Trapezium-Type Inequalities for Generalized ϕ-Convex Functions
Authors: Vivas-Cortez Miguel J. , Liko Rozana , Kashuri Artion , Hernández Hernández Jorge E.
Published: November 2019

In this paper, a quantum trapezium-type inequality using a new class of function, the so-called generalized ϕ -convex function, is presented. A new quantum trapezium-type inequality for the product of two generalized ϕ -convex functions is provided. The authors also prove an identity for twice q - differentiable functions using Raina’s function. Utilizing the identity established, certain quantum estimated inequalities for the above class are developed. Various special cases have been studied. A brief conclusion is also given.
An Optimisation-Driven Prediction Method for Automated Diagnosis and Prognosis
Authors: Santucci Valentino , Milani Alfredo , Caraffini Fabio
Published: November 2019

This article presents a novel hybrid classification paradigm for medical diagnoses and prognoses prediction. The core mechanism of the proposed method relies on a centroid classification algorithm whose logic is exploited to formulate the classification task as a real-valued optimisation problem. A novel metaheuristic combining the algorithmic structure of Swarm Intelligence optimisers with the probabilistic search models of Estimation of Distribution Algorithms is designed to optimise such a problem, thus leading to high-accuracy predictions. This method is tested over 11 medical datasets and compared against 14 cherry-picked classification algorithms. Results show that the proposed approach is competitive and superior to the state-of-the-art on several occasions.
Econophysics and Fractional Calculus: Einstein’s Evolution Equation, the Fractal Market Hypothesis, Trend Analysis and Future Price Prediction
Authors: Blackledge Jonathan , Kearney Derek , Lamphiere Marc , Rani Raja , Walsh Paddy
Published: November 2019

This paper examines a range of results that can be derived from Einstein’s evolution equation focusing on the effect of introducing a Lévy distribution into the evolution equation. In this context, we examine the derivation (derived exclusively from the evolution equation) of the classical and fractional diffusion equations, the classical and generalised Kolmogorov–Feller equations, the evolution of self-affine stochastic fields through the fractional diffusion equation, the fractional Poisson equation (for the time independent case), and, a derivation of the Lyapunov exponent and volatility. In this way, we provide a collection of results (which includes the derivation of certain fractional partial differential equations) that are fundamental to the stochastic modelling associated with elastic scattering problems obtained under a unifying theme, i.e., Einstein’s evolution equation. This includes an analysis of stochastic fields governed by a symmetric (zero-mean) Gaussian distribution, a Lévy distribution characterised by the Lévy index γ ∈ [ 0 , 2 ] and the derivation of two impulse response functions for each case. The relationship between non-Gaussian distributions and fractional calculus is examined and applications to financial forecasting under the fractal market hypothesis considered, the reader being provided with example software functions (written in MATLAB) so that the results presented may be reproduced and/or further investigated.
A Continuation Procedure for the Quasi-Static Analysis of Materially and Geometrically Nonlinear Structural Problems
Authors: Bellora Davide , Vescovini Riccardo
Published: November 2019

Discussed is the implementation of a continuation technique for the analysis of nonlinear structural problems, which is capable of accounting for geometric and dissipative requirements. The strategy can be applied for solving quasi-static problems, where nonlinearities can be due to geometric or material response. The main advantage of the proposed approach relies in its robustness, which can be exploited for tracing the equilibrium paths for problems characterized by complex responses involving the onset and propagation of cracks. A set of examples is presented and discussed. For problems involving combined material and geometric nonlinearties, the results illustrate the advantages of the proposed hybrid continuation technique in terms of efficiency and robustness. Specifically, less iterations are usually required with respect to similar procedures based on purely geometric constraints. Furthermore, bifurcation plots can be easily traced, furnishing the analyst a powerful tool for investigating the nonlinear response of the structure at hand.
Exercise Interventions for Improving Flexibility in People with Multiple Sclerosis: A Systematic Review and Meta-Analysis
Authors: Torres-Pareja Marta , Sánchez-Lastra Miguel A. , Iglesias Laura , Suárez-Iglesias David , Mendoza Nuria , Ayán Carlos
Published: November 2019

Background and objectives: People with multiple sclerosis (MS) often experience limitations in joint range of motion, which is linked to spasticity and continued inactivity. Low flexibility levels in this population have been linked to postural problems and muscular pain. Therefore, the purpose of this study was to conduct a systematic review and a meta-analysis aimed at identifying the characteristics and methodological quality of investigations studying the effects of exercise interventions on the flexibility levels of people with MS. Materials and Methods: Three electronic databases (MEDLINE/PubMed, SPORTDiscus and Scopus) were systematically searched up to May 2019 for intervention studies focused on the effects of exercise on the flexibility levels of people with MS. A meta-analysis, including randomized controlled trials (RCT), which reported information regarding the effects of exercise on flexibility, was also conducted. The methodological quality of included studies was assessed using the Physiotherapy Evidence Database, and the Quality Assessment Tool for Before–After Studies, with no control group. The quality of the information reported, regarding the programs conducted, was assessed by means of the Consensus on Exercise Reporting Template (CERT) scale. Results: Seven studies, four RCTs and three uncontrolled investigations were finally selected. The methodological quality of the RCTs was considered “poor” in one study, and “good” and “excellent” in two studies and one investigation, respectively. The three uncontrolled studies showed a methodological quality between “fair” and “poor”. Following the CERT scale, four studies were graded as “high” and three as “low”. Findings from the meta-analysis indicated no significant effects on hamstring flexibility, or the range of motion in the hips, knees or ankles. Conclusions: There is preliminary evidence from individual studies which indicates that people with MS can improve their lower limb flexibility following participation in physical exercise programs, but the meta-analysis did not confirm these findings.
Long-Term Exposure of Psoralen and Isopsoralen Induced Hepatotoxicity and Serum Metabolites Profiles Changes in Female Rats
Authors: Yu Yingli , Wang Pengli , Yu Ruili , Lu Jiaxi , Jiang Miaomiao , Zhou Kun
Published: November 2019

Pre-clinical safety evaluation of traditional medicines is imperative because of the universality of drug-induced adverse reactions. Psoralen and isopsoralen are the major active molecules and quality-control components of a traditional herbal medicine which is popularly used in Asia, Fructus Psoraleae. The purpose of this study is to assess the long-term effects of psoralen and isopsoralen with low levels on the biochemical parameters and metabolic profiles of rats. Three doses (14, 28, and 56 mg/kg) of psoralen and one dose (28 mg/kg) of isopsoralen were administered to rats over 12 weeks. Blood and selected tissue samples were collected and analyzed for hematology, serum biochemistry, and histopathology. Metabolic changes in serum samples were detected via proton nuclear magnetic resonance (1H-NMR) spectroscopy. We found that psoralen significantly changed the visceral coefficients, blood biochemical parameters, and histopathology, and isopsoralen extra influenced the hematological index. Moreover, psoralen induced remarkable elevations of forvaline, isoleucine, isobutyrate, alanine, acetone, pyruvate, glutamine, citrate, unsaturated lipids, choline, creatine, phenylalanine, and 4-hydroxybenzoate, and significant reductions of ethanol and dimethyl sulfone. Isopsoralen only induced a few remarkable changes of metabolites. These results suggest that chronic exposure to low-level of psoralen causes a disturbance in alanine metabolism, glutamate metabolism, urea cycle, glucose-alanine cycle, ammonia recycling, glycine, and serine metabolism pathways. Psoralen and isopsoralen showed different toxicity characteristics to the rats.
Seasonal Variations and Interspecific Differences in Metabolomes of Freshwater Fish Tissues: Quantitative Metabolomic Profiles of Lenses and Gills
Authors: Tsentalovich Yuri P. , Yanshole Vadim V. , Yanshole Lyudmila V. , Zelentsova Ekaterina A. , Melnikov Arsenty D. , Sagdeev Renad Z.
Published: November 2019

This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.
Microbiological Assessment of the Quality of Some Commercial Products Marketed as Lactobacillus crispatus-Containing Probiotic Dietary Supplements
Authors: Di Pierro Francesco , Polzonetti Valeria , Patrone Vania , Morelli Lorenzo
Published: November 2019

In the last decade, many authors have reported low viability for probiotic products. Investigators commonly find they are not meeting claimed active counts and/or incorrect species and/or strains have been identified. We have therefore decided to verify viability, the real dose and species correspondence in nine probiotic products (seven nutritional supplements and two medical devices) collected from the Italian and French markets claiming to contain at least one strain of L. crispatus among the different species/strain included in the formulation. In fact, the medical relevance of L. crispatus strains has recently grown., as evaluating the possible dominance clusters typical of the vaginal microbiota, the Community State Type I, the one dominated by L. crispatus, appears to be “protective” in terms of infections, fertility and gestational duration of pregnancy. The results obtained demonstrate the generally poor quality of probiotics. Out of nine products, only two definitely contained viable Lactobacillus crispatus cells with a daily dose of at least 1 × 109 CFU/g and with an acceptable correspondence with what is declared on the label. Among these two, only one was found to be formulated with a strain (M247) that has been scientifically documented.
Distinct Microbial Community of Phyllosphere Associated with Five Tropical Plants on Yongxing Island, South China Sea
Authors: Bao Lijun , Cai Wenyang , Zhang Xiaofen , Liu Jinhong , Chen Hao , Wei Yuansong , Jia Xiuxiu , Bai Zhihui
Published: November 2019

The surfaces of a leaf are unique and wide habitats for a microbial community. These microorganisms play a key role in plant growth and adaptation to adverse conditions, such as producing growth factors to promote plant growth and inhibiting pathogens to protect host plants. The composition of microbial communities very greatly amongst different plant species, yet there is little data on the composition of the microbiome of the host plants on the coral island in the South China Sea. In this study, we investigated the abundances and members of a major microbial community (fungi, bacteria, and diazotrophs) on the leaves of five dominant plant species (Ipomoea pes-caprae, Wedelia chinensis, Scaevola sericea, Cocos nucifera, and Sesuvium portulacastrum) on the island using real-time quantitative polymerase chain reaction (PCR) and high-throughput amplicon sequencing. Quantitative PCR results showed that fungi and bacteria were ubiquitous and variable among different host plants. Scaevola sericea showed the lowest absolute abundance and highest diversity of fungi and bacteria, while Cocos nucifera had the lowest abundance and the highest diversity of diazotrophs compare to the other four plants. There was a small proportion of shared microorganisms among the five different plants, while unique fungi, bacteria and diazotrophs were significantly enriched for different host plant species in this study (p < 0.05). Some of the most abundant organisms found in the communities of these different host plants are involved in important biogeochemical cycles that can benefit their host, including carbon and nitrogen cycles.
Relative Abundance of Ammonia Oxidizing Archaea and Bacteria Influences Soil Nitrification Responses to Temperature
Authors: Mukhtar Hussnain , Lin Yu-Pin , Lin Chiao-Ming , Lin Yann-Rong
Published: November 2019

Ammonia oxidizing archaea (AOA) and bacteria (AOB) are thought to contribute differently to soil nitrification, yet the extent to which their relative abundances influence the temperature response of nitrification is poorly understood. Here, we investigated the impact of different AOA to AOB ratios on soil nitrification potential (NP) across a temperature gradient from 4 °C to 40 °C in twenty different organic and inorganic fertilized soils. The temperature responses of different relative abundance of ammonia oxidizers for nitrification were modeled using square rate theory (SQRT) and macromolecular rate theory (MMRT) models. We found that the proportional nitrification rates at different temperatures varied among AOA to AOB ratios. Predicted by both models, an optimum temperature (Topt) for nitrification in AOA dominated soils was significantly higher than for soils where AOA and AOB abundances are within the same order of magnitude. Moreover, the change in heat capacity ( Δ C P ‡ ) associated with the temperature dependence of nitrification was positively correlated with Topt and significantly varied among the AOA to AOB ratios. The temperature ranges for NP decreased with increasing AOA abundance for both organic and inorganic fertilized soils. These results challenge the widely accepted approach of comparing NP rates in different soils at a fixed temperature. We conclude that a shift in AOA to AOB ratio in soils exhibits distinguished temperature-dependent characteristics that have an important impact on nitrification responses across the temperature gradient. The proposed approach benefits the accurate discernment of the true contribution of fertilized soils to nitrification for improvement of nitrogen management.
Texture Development and Stress–Strain Partitioning in Periclase + Halite Aggregates
Authors: Lin Feng , Giannetta Max , Jugle Mike , Couper Samantha , Dunleavy Becky , Miyagi Lowell
Published: November 2019

Multiphase materials are widely applied in engineering due to desirable mechanical properties and are of interest to geoscience as rocks are multiphase. High-pressure mechanical behavior is important for understanding the deep Earth where rocks deform at extreme pressure and temperature. In order to systematically study the underlying physics of multiphase deformation at high pressure, we perform diamond anvil cell deformation experiments on MgO + NaCl aggregates with varying phase proportions. Lattice strain and texture evolution are recorded using in-situ synchrotron x-ray diffraction and are modeled using two-phase elasto-viscoplastic self-consistent (EVPSC) simulations to deduce stress, strain, and deformation mechanisms in individual phases and the aggregate. Texture development of MgO and NaCl are affected by phase proportions. In NaCl, a (100) compression texture is observed when small amounts of MgO are present. In contrast, when deformed as a single phase or when large amounts of MgO are present, NaCl develops a (110) texture. Stress and strain evolution in MgO and NaCl also show different trends with varying phase proportions. Based on the results from this study, we construct a general scheme of stress evolution as a function of phase proportion for individual phases and the aggregate.
A Generic Method for Fast and Sensitive Detection of Adeno-Associated Viruses Using Modified AAV Receptor Recombinant Proteins
Authors: Cui Mengtian , Lu Yabin , Tang Can , Zhang Ran , Wang Jing , Si Yang , Cheng Shan , Ding Wei
Published: November 2019

Adeno-Associated Viruses (AAV) are widely used gene-therapy vectors for both clinical applications and laboratory investigations. The titering of different AAV preparations is important for quality control purposes, as well as in comparative studies. However, currently available methods are limited in their ability to detect various serotypes with sensitivity and convenience. Here, we took advantage of a newly discovered AAV receptor protein with high affinity to multiple AAV serotypes, and developed an ELISA-like method named “VIRELISA” (virus receptor-linked immunosorbent assay) by adopting fusion with a streptavidin-binding peptide (SBP). It was demonstrated that optimized VIRELISA assays exhibited satisfactory performance for the titering of AAV2. The linear range of AAV2 was 1 × 105 v.g. to 5 × 109 v.g., with an LOD (limit of detection) of 5 × 104 v.g. Testing of VIRELISA for the quantification of AAV1 was also successful. Our study indicated that a generic protocol for the quantification of different serotypes of AAVs was feasible, reliable and cost-efficient. The applications of VIRELISA will not only be of benefit to laboratory research due to its simplicity, but could also potentially be used for monitoring the circulation AAV loads both in clinical trials and in wild type infection of a given AAV serotype.
Differential Cytotoxic Potential of Acridocarpus orientalis Leaf and Stem Extracts with the Ability to Induce Multiple Cell Death Pathways
Authors: Balhamar Sameera Omar Mohammed Saeed , Panicker Neena Gopinathan , Akhlaq Shaima , Qureshi Mohammed Mansoor , Ahmad Waqar , Rehman Najeeb Ur , Ali Liaqat , Al-Harrasi Ahmed , Hussain Javid , Mustafa Farah
Published: November 2019

This study systematically analyzed the anticancer potential of Acridocarpus orientalis (AO), a traditional medicinal plant of the Arabian Peninsula/East Africa known for its anti-inflammatory and pain relief properties. Tests of serial organic fractions from methanolic extracts of its leaves and stems revealed that only some fractions showed anti-proliferative potential with the dichloromethane fraction from leaves (AOD (L)) showing the most cytotoxic effect against both breast (MCF-7 and MDA-MB-231) and cervical (HeLa) cancer cell lines. The n-butanol fraction from the stems (AOB (S)), on the other hand, was more effective against cervical cancer cells and did not harm the normal cells. Further characterization of the mode of cell killing revealed that AOD (L) depended more on non-apoptotic pathways for its cytotoxicity in breast cancer cells, while it could activate some apoptosis and necroptosis in HeLa cells. The AOB (S) fraction could primarily activate apoptosis and some necroptosis in HeLa cells. Both fractions perturbed autophagy, but in a dissimilar manner. Thus, different parts of A. orientalis revealed variable potential to induce cell death in cancer cells via apoptotic and non-apoptotic pathways, making A. orientalis a valuable plant for the exploration of anticancer bioactive reagents, some of which may be protective for normal cells.
Use of Deep Eutectic Solvents in Polymer Chemistry–A Review
Authors: Jablonský Michal , Škulcová Andrea , Šima Jozef
Published: November 2019

This review deals with two overlapping issues, namely polymer chemistry and deep eutectic solvents (DESs). With regard to polymers, specific aspects of synthetic polymers, polymerization processes producing such polymers, and natural cellulose-based nanopolymers are evaluated. As for DESs, their compliance with green chemistry requirements, their basic properties and involvement in polymer chemistry are discussed. In addition to reviewing the state-of-the-art for selected kinds of polymers, the paper reveals further possibilities in the employment of DESs in polymer chemistry. As an example, the significance of DES polarity and polymer polarity to control polymerization processes, modify polymer properties, and synthesize polymers with a specific structure and behavior, is emphasized.
Eugenol Exerts Apoptotic Effect and Modulates the Sensitivity of HeLa Cells to Cisplatin and Radiation
Authors: Fathy Moustafa , Fawzy Michael Atef , Hintzsche Henning , Nikaido Toshio , Dandekar Thomas , Othman Eman M.
Published: November 2019

Eugenol is a phytochemical present in different plant products, e.g., clove oil. Traditionally, it is used against a number of different disorders and it was suggested to have anticancer activity. In this study, the activity of eugenol was evaluated in a human cervical cancer (HeLa) cell line and cell proliferation was examined after treatment with various concentrations of eugenol and different treatment durations. Cytotoxicity was tested using lactate dehydrogenase (LDH) enzyme leakage. In order to assess eugenol’s potential to act synergistically with chemotherapy and radiotherapy, cell survival was calculated after eugenol treatment in combination with cisplatin and X-rays. To elucidate its mechanism of action, caspase-3 activity was analyzed and the expression of various genes and proteins was checked by RT-PCR and western blot analyses. Eugenol clearly decreased the proliferation rate and increased LDH release in a concentration- and time-dependent manner. It showed synergistic effects with cisplatin and X-rays. Eugenol increased caspase-3 activity and the expression of Bax, cytochrome c (Cyt-c), caspase-3, and caspase-9 and decreased the expression of B-cell lymphoma (Bcl)-2, cyclooxygenase-2 (Cox-2), and interleukin-1 beta (IL-1β) indicating that eugenol mainly induced cell death by apoptosis. In conclusion, eugenol showed antiproliferative and cytotoxic effects via apoptosis and also synergism with cisplatin and ionizing radiation in the human cervical cancer cell line.
Protein Chemical Labeling Using Biomimetic Radical Chemistry
Authors: Sato Shinichi , Nakamura Hiroyuki
Published: November 2019

Chemical labeling of proteins with synthetic low-molecular-weight probes is an important technique in chemical biology. To achieve this, it is necessary to use chemical reactions that proceed rapidly under physiological conditions (i.e., aqueous solvent, pH, low concentration, and low temperature) so that protein denaturation does not occur. The radical reaction satisfies such demands of protein labeling, and protein labeling using the biomimetic radical reaction has recently attracted attention. The biomimetic radical reaction enables selective labeling of the C-terminus, tyrosine, and tryptophan, which is difficult to achieve with conventional electrophilic protein labeling. In addition, as the radical reaction proceeds selectively in close proximity to the catalyst, it can be applied to the analysis of protein–protein interactions. In this review, recent trends in protein labeling using biomimetic radical reactions are discussed.
Gamma Irradiated Rhodiola sachalinensis Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Downregulating 5-Alpha Reductase and Restoring Testosterone in Rats
Authors: Xin Qi , Kwon Mi-Jin , Lee Ju-Woon , Kim Kwan-Soo , Chen Hao , Campos Maria G. , Tundis Rosa , Cui Cheng-Bi , Cho Young Ho , Cao Hui
Published: November 2019

The effect of Rhodiola sachalinensis Boriss extract irradiated with 50 kGy gamma rays (HKC) on benign prostatic hyperplasia (BPH) was investigated. Seven-week-old male SD rats received a subcutaneous injection of 20 mg/kg of testosterone propionate (TP) to induce BPH. Then, the testosterone only group received testosterone, the testosterone + finasteride group received testosterone and finasteride (5 mg/kg), the testosterone + HKC group received testosterone and HKC extract (500 mg/kg). Prostate weight and the dihydrotestosterone (DHT) levels in serum or prostate tissue were determined. The mRNA expressions of 5-alpha reductase (AR) in prostate tissue were also measured. Compared to the control group, prostate weight was significantly improved in the TP group and decreased in the HKC and finasteride-treated groups. Furthermore, the mRNA expression of 5-AR in the prostate was significantly reduced in the HKC and finasteride-treated groups. Similarly, the expression levels of α-smooth muscle actin (α-SMA) and cytokeratin, which are associated with prostatic enlargement in the HKC and finasteride groups, were much lower than in the TP group. HKC treatment showed similar efficacy to finasteride treatment on rats with testosterone-induced BPH. HKC may be explored as a potential new drug for BPH treatment.
NaYF4 Microstructure, beyond Their Well-Shaped Morphology
Authors: Leménager Godefroy , Tusseau-Nenez Sandrine , Thiriet Maud , Coulon Pierre-Eugène , Lahlil Khalid , Larquet Eric , Gacoin Thierry
Published: November 2019

Lanthanide-doped nanoparticles are widely investigated for their optical properties. However, the sensitivity of the lanthanide ions’ luminescence to the local symmetry, useful when investigating structural environments, becomes a drawback for optimized properties in the case of poorly controlled crystallinity. In this paper, we focus on β -NaYF4 nanorods in order to provide a detailed description of their chemical composition and microstructure. The combination of detailed XRD analysis and TEM observations show that strong variation may be observed from particles from a same batch of synthesis, but also when considering small variations of synthesis conditions. Moreover, also the nanorods observed by SEM exhibit a very nice faceted shape, they are far from being monocrystalline and present significant local deviation of crystalline symmetry and orientation. All these structural considerations, sensitively probed by polarized emission analysis, are crucial to analyze for the development of optimal systems toward the targeted applications.
Effects of Embedded Helium on the Microstructure and Mechanical Properties of Erbium Films
Authors: Fu Wenbo , Shen Huahai , Shi Liqun , Zhou Xiaosong , Long Xinggui
Published: November 2019

A series of helium (He) charged nanograin-sized erbium (Er) films were deposited by direct current (DC)-magnetron sputtering with different He/Ar mixture gases. The microstructure and mechanical properties of He-charged Er films were investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM), and nanoindentation. The helium concentrations in Er films, determined by elastic recoil detection analysis (ERDA), ranged from 0 to 49.6%, with the increase in He:Ar flow ratio up to 18:1. The XRD results show that the grain sizes of Er films decreased with and increase in He content. The embedded He atoms induced the formation of spherical nanometer He bubbles, and the diameter of the He bubbles increased with the He content. The hardness and Young’s modulus increased and decreased with the decreasing grain sizes of polycrystalline Er–He films. The mechanisms of mechanical properties with respect to the grain size and He content were discussed based on the Hall–Petch formula and composite spheres model.
In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
Authors: Turin-Moleavin Ioana-Andreea , Fifere Adrian , Lungoci Ana-Lacramioara , Rosca Irina , Coroaba Adina , Peptanariu Dragos , Nastasa Valentin , Pasca Sorin-Aurelian , Bostanaru Andra-Cristina , Mares Mihai , Pinteala Mariana
Published: November 2019

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.
Small RNA Species and microRNA Profiles are Altered in Severe Asthma Nanovesicles from Broncho Alveolar Lavage and Associate with Impaired Lung Function and Inflammation
Authors: Francisco-Garcia Ana S. , Garrido-Martín Eva M. , Rupani Hitasha , Lau Laurie C. K. , Martinez-Nunez Rocio T. , Howarth Peter H. , Sanchez-Elsner Tilman
Published: November 2019

MicroRNAs are known to regulate important pathways in asthma pathology including the IL-6 and IFN pathways. MicroRNAs have been found not only within cells but also within extracellular vesicles such as exosomes. In this study, we particularly focused on microRNA cargo of nanovesicles in bronchoalveolar lavage of severe asthmatic patients. We extracted nanovesicle RNA using a serial filtration method. RNA content was analyzed with small RNA sequencing and mapped to pathways affected using WebGestalt 2017 Software. We report that severe asthma patients have deficient loading of microRNAs into their airway luminal nanovesicles and an altered profile of small RNA nanovesicle content (i.e., ribosomal RNA and broken transcripts, etc.). This decrease in microRNA cargo is predicted to increase the expression of genes by promoting inflammation and remodeling. Consistently, a network of microRNAs was associated with decreased FEV1 and increased eosinophilic and neutrophilic inflammation in severe asthma. MicroRNAs in airway nanovesicles may, thus, be valid biomarkers to define abnormal biological disease processes in severe asthma and monitor the impact of interventional therapies.
Assessment of the Genetic Diversity of Rice Germplasms Characterized by Black-Purple and Red Pericarp Color Using Simple Sequence Repeat Markers
Authors: Park Jae-Ryoung , Yang Won-Tae , Kwon Yong-Sham , Kim Hyeon-Nam , Kim Kyung-Min , Kim Doh-Hoon
Published: November 2019

The assessment of the genetic diversity within germplasm collections can be accomplished using simple sequence repeat (SSR) markers and association mapping techniques. The present study was conducted to evaluate the genetic diversity of a colored rice germplasm collection containing 376 black-purple rice samples and 172 red pericarp samples, conserved by Dong-A University. There were 600 pairs of SSR primers screened against 11 rice varieties. Sixteen informative primer pairs were selected, having high polymorphism information content (PIC) values, which were then used to assess the genetic diversity within the collection. A total of 409 polymorphic amplified fragments were obtained using the 16 SSR markers. The number of alleles per locus ranged from 11 to 47, with an average of 25.6. The average PIC value was 0.913, ranging from 0.855 to 0.964. Four hundred and nine SSR loci were used to calculate Jaccard’s distance coefficients, using the unweighted pair-group method with arithmetic mean cluster analysis. These accessions were separated into several distinctive groups corresponding to their morphology. The results provided valuable information for the colored rice breeding program and showed the importance of protecting germplasm resources and the molecular markers that can be derived from them.
Research on the Vertical Vibration Characteristics of Hydraulic Screw Down System of Rolling Mill under Nonlinear Friction
Authors: Zhang Yongshun , Jiang Wanlu , Zhu Yong , Li Zhenbao
Published: November 2019

The rolling mill with hydraulic system is widely used in the production of strip steel. For the problem of vertical vibration of the rolling mill, the effects of different equivalent damping coefficient, leakage coefficient, and proportional coefficient of the controller on the hydraulic screw down system of the rolling mill are studied, respectively. First, a vertical vibration model of a hydraulic screw down system was established, considering the nonlinear friction and parameter uncertainty of the press cylinder. Second, the correlation between different equivalent damping coefficient, internal leakage coefficient, proportional coefficient, vertical vibration was analyzed. The simulation results show that, in the closed-loop state, when Proportional-Integral-Derivative (PID) controller parameters are fixed, due to the change of the equivalent damping coefficient and internal leakage coefficient, the system will have parameter uncertainty, which may lead to the failure of the PID controller and the vertical vibration of the system. This study has theoretical and practical significance for analyzing the mechanism of vertical vibration of the rolling mill.