bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimycobacterial Activity of Hexapeptide Related to Apidaecin IA
Authors: Perumal Pandurangan , Seenivasan Prabhu
Published: November 2019

Background: Apidaecin IA is an animal peptide obtained from honey bee having 18 amino acid presents in the sequence of the peptide. Objective: To design, synthesis, characterization, evaluation of antimycobacterial peptide related to apidaecin IA. Methods; Synthesis by combinatorial chemistry using FMOC chemistry protocol, characterized by mass spectroscopy and sequence analysis using LC-MS-MS and evaluated antifungal, antimicrobial activity by zone of inhibition and minimum inhibitory concentration and antimycobacterial activity by Luciferase Reporter Phage (LRP) assay.
A systematic review of analytical methods for the detection and quantification of β-N-methylamino-l-alanine (BMAA)
Authors: Bishop Stephanie L. , Murch Susan J.
Published: November 2019

A review and critical analysis of analytical methods used to quantify the neurotoxin β-N-methylamino-l-alanine in four typically studied sample matrices.
Metal-involving halogen bond Ar–I⋯[dz2PtII] in a platinum acetylacetonate complex
Authors: Rozhkov Anton V. , Ivanov Daniil M. , Novikov Alexander S. , Ananyev Ivan V. , Bokach Nadezhda A. , Kukushkin Vadim Yu.
Published: November 2019

The observed and confirmed theoretically metal-involving halogen bond Ar–I⋯[dz2PtII] provides experimental evidence favoring a XB formation step upon oxidative addition of ArI to PtII.
On the ionic liquid films ‘pinned’ by core–shell structured Fe3O4@carbon nanoparticles and their tribological properties
Authors: An Rong , Wu Muqiu , Li Jing , Qiu Xiuhua , Shah Faiz Ullah , Li Jianliang
Published: November 2019

Fe3O4@C improving ionic liquid tribological properties: Fe3O4-cores with dipole attractions to anchor the liquid and carbon-shells to resist aggregation.
An effective approach to achieve high energy storage density and efficiency in BNT-based ceramics by doping AgNbO3
Authors: Wang Hua , Jiang Xiaoli , Liu Xiaoqin , Yang Ruonan , Yang Yang , Zheng Qiaoji , Kwok K. W. , Lin Dunmin
Published: November 2019

The BNBLT–0.01AN ceramic with the highest energy storage density (Ws) value of ∼1.697 J cm−3 and energy storage efficiency (η) of ∼82.3% exhibits optimal thermal stability and frequency stability.
Rapid synthesis and NH3-SCR activity of SSZ-13 zeolite via coal gangue
Authors: Han Jinfeng , Jin Xiaotong , Song Chunfeng , Bi Yalian , Liu Qingling , Liu Caixia , Ji Na , Lu Xuebin , Ma Degang , Li Zhenguo
Published: November 2019

The presence of tetrahedral aluminum was the main factor contributing to the rapid synthesis, which also promoted the transformation from octahedral aluminum to tetrahedral aluminum structure unit, and further accelerated the formation of SSZ-13.
Synthesis, characterization, and antitumor properties of Au(i)–thiourea complexes
Authors: Yu Bingqiong , Liu Yanhong , Peng Xian , Hua Siyu , Zhou Gangcheng , Yan Kun , Liu Yi
Published: November 2019

The anticancer property of cisplatin has stimulated the development of metal complexes as antitumor agents. Among these complexes, metal thiourea complexes have attracted sufficient attention, and they possess the potential possibility to become new antitumor metallodrugs. Herein, four Au(i) complexes derived from N,N-disubstituted cyclic thiourea ligands were synthesized and characterized. The crystal structure analysis indicated that the complex Au(i)(3c)2OTf was a mononuclear crystal structure with Au(i) coordinated by two sulfur atoms. These Au(i) complexes exhibited excellent toxicities against several tumor cell lines, especially complex Au(i)(3c)2OTf (IC50 = 8.06 μM against HeLa). It was found that Au(i)(3c)2OTf triggered a burst of ROS, disrupted the mitochondrial membrane potential (MMP), subsequently released Cyt-c, and then triggered the activation of caspase 9, caspase 7 and caspase 3. Mechanism experiments manifested that Au(i)(3c)2OTf induced the down-regulation of Bcl-2 and up-regulation of Bax, which further indicated that Au(i)(3c)2OTf triggered mitochondria-mediated apoptosis. In addition, the ROS scavenger-NAC completely blocked the apoptosis and inhibited the reduction of MMP, showing that Au(i)(3c)2OTf induced a ROS-dependent apoptosis pathway. These results indicate that Au(i)(3c)2OTf is worthy of in-depth research as an antitumor agent and may throw light on a better understanding of the effect of thiourea derivatives on antitumor mechanisms.
Controlled assembly of artificial 2D materials based on the transfer of oxo-functionalized graphene
Authors: Hußmann Marleen , Weintrub Benjamin , Feicht Patrick , Germer Gregor , Kirchhof Jan N. , Bolotin Kirill I. , Eigler Siegfried
Published: November 2019

A method is described to transfer and integrate transparent and polar 2D materials into heterostructures.
Highly active mesoionic chalcogenone zinc(ii) derivatives for C–S cross-coupling reactions
Authors: Vaddamanu Moulali , Velappan Kavitha , Prabusankar Ganesan
Published: November 2019

Mesoionic heavier chalcogenone complexes of zinc(ii) have been isolated and utilized as catalysts in C–S cross coupling reactions between thiophenols and aryl halides under convenient reaction conditions.
Self-assembled star-shaped aza-BODIPY mesogen affords white-light emission
Authors: Liu Chao , Ding Wei , Liu Yuantao , Zhao Hongmei , Cheng Xiaohong
Published: November 2019

A star-shaped aza-BODIPY mesogen exhibits LC, gel, WLE and chemosensor properties.
Peroxynitrite (ONOO−) generation from the HA-TPP@NORM nanoparticles based on synergistic interactions between nitric oxide and photodynamic therapies for elevating anticancer efficiency
Authors: Jiang Dawei , Yue Tao , Wang Guichen , Wang Chaochao , Chen Chao , Cao Hongliang , Gao Yun
Published: November 2019

Due to biological safety and negligible toxicity, nitric oxide (NO) therapy has gained increasing interest in the field of cancer therapy during the past few years.
Revealing the potential application of chiral covalent organic frameworks in CO2 adsorption and separation
Authors: Liu Shuang , Li Wen-Liang , Zhang Jing-Ping
Published: November 2019

This work uses theoretical calculations to discover the potential application of CCOFs in gas adsorption and separation, and conduct theoretical research.
Synthesis and vitamin D receptor affinity of 16-oxa vitamin D3analogues
Authors: Ibe Kouta , Yamada Takeshi , Okamoto Sentaro
Published: November 2019

Two novel 16-oxa-vitamin D3analogues were synthesized using a Ti(ii)-mediated enyne cyclization/Cu-catalyzed allylation, Ru-catalyzed ring-closing metathesis, and a titanium-mediated stereoselective radical reduction of epoxide as the key steps.
Increased conjugated backbone twisting to improve carbonylated-functionalized polymer photovoltaic performance
Authors: Zhang Tao , An Cunbin , Ma Kangqiao , Xian Kaihu , Xue Changguo , Zhang Shaoqing , Xu Bowei , Hou Jianhui
Published: November 2019

Two conjugated polymers containing different linkers were synthesized to study their photovoltaic performances.
Conical microstructuring of titanium by reactive gas assisted laser texturing
Authors: Wöbbeking Karl , Li Mingji , Hübner Eike G. , Schade Wolfgang
Published: November 2019

Microstructuring of titanium with high-power laser systems can be controlled by halogens as additives.
Retracted Article: Exosome-derived PTENP1 suppresses cisplatin resistance of bladder cancer (BC) by suppressing cell proliferation, migration and inducing apoptosis via the miR-103a/PDCD4 axis
Authors: Lu Xingre , Chen Fengyu , Yuan Diao , He Xiang , Liu Xiaowen , Zi Yunju , Lu Yu
Published: November 2019

Bladder cancer (BC) is a lethal cancer that threatens the health of millions of people.
Pathways connecting two opposed bilayers with a fusion pore: a molecularly-informed phase field approach
Authors: Han Yucen , Xu Zirui , Shi An-Chang , Zhang Lei
Published: November 2019

A phase field model with two phase fields, representing the concentration and the head–tail separation of amphiphilic molecules, respectively, has been constructed using an extension of the Ohta–Kawasaki model (Macromolecules, 1986, 19, 2621–2632).
Poly-amino acids coated gold nanorod and doxorubicin for synergistic photodynamic therapy and chemotherapy in ovarian cancer cells
Authors: Liu JinYing , Ma Wei , Kou Wei , Shang Lina , Huang Rui , Zhao Jin
Published: November 2019

In this work, we have successfully designed and formulated a doxorubicin-loaded polypeptide-based multilayer assembled gold nanorod (DH-GNR). We have hypothesized that near-infrared (NIR) laser irradiation of DH-GNR will combine the benefits of chemotherapy and photothermal therapy. The GNR was surface functionalized with poly-glutamic acid (PGA) and poly-l-Lysine (PLL) with a final layer of hyaluronic acid (HA) that could also serve as a targeting ligand toward the overexpressed CD44 receptors in ovarian cancer cells. The zigzag ζ potential of nanoparticle is a proof of successful assembly of alternative polymers on the GNR surface. NIR irradiation exhibited a burst release of drug in pH 7.4 and pH 5.0 buffer conditions. The combination of doxorubicin (DOX)-based chemotherapy and GNR-based photothermal therapy exhibited a synergistic effect in killing the SKOV3 cancer cells. DH-GNR(+NIR) induced a 82.5% apoptosis (combined early and late apoptosis) compared with only 35.2 and 38.5% for DOX or DH-GNR(−NIR) treated cell group. Results clearly suggest that the excessive reactive oxygen species (ROS) generation in DH-GNR (+NIR) might be responsible for the cell apoptosis and cell death. The promising anticancer effect of DH-GNR will be of great potential in the treatment of ovarian cancers and worth further development for treating other malignant tumors.
Combined in silico and 19F NMR analysis of 5-fluorouracil metabolism in yeast at low ATP conditions
Authors: Pawłowski Piotr H. , Szczęsny Paweł , Rempoła Bożenna , Poznańska Anna , Poznański Jarosław
Published: November 2019

The cytotoxic effect of 5-fluorouracil (5-FU) on yeast cells is thought to be mainly via a misincorporation of fluoropyrimidines into both RNA and DNA, not only DNA damage via inhibition of thymidylate synthase (TYMS) by fluorodeoxyuridine monophosphate (FdUMP). However, some studies on Saccharomyces cerevisiae show a drastic decrease in ATP concentration under oxidative stress, together with a decrease in concentration of other tri- and diphosphates. This raises a question if hydrolysis of 5-fluoro-2-deoxyuridine diphosphate (FdUDP) under oxidative stress could not lead to the presence of FdUMP and the activation of so-called ‘thymine-less death’ route. We attempted to answer this question with in silico modeling of 5-FU metabolic pathways, based on new experimental results, where the stages of intracellular metabolism of 5-FU in Saccharomyces cerevisiae were tracked by a combination of 19F and 31P NMR spectroscopic study. We have identified 5-FU, its nucleosides and nucleotides, and subsequent di- and/or triphosphates. Additionally, another wide 19F signal, assigned to fluorinated unstructured short RNA, has been also identified in the spectra. The concentration of individual metabolites was found to vary substantially within hours, however, the initial steady-state was preserved only for an hour, until the ATP concentration dropped by a half, which was monitored independently via 31P NMR spectra. After that, the catabolic process leading from triphosphates through monophosphates and nucleosides back to 5-FU was observed. These results imply careful design and interpretation of studies in 5-FU metabolism in yeast.
On visions and promises — ethical aspects of in vitro meat
Authors: Woll Silvia
Published: November 2019

Innovators of in vitro meat (IVM) are convinced that this approach is the solution for problems related to current meat production and consumption, especially regarding animal welfare and environmental issues. However, the production conditions have yet to be fully clarified and there is still a lack of ethical discourses and critical debates on IVM. In consequence, discussion about the ethical justifiability and desirability of IVM remains hypothetical and we have to question those promises. This paper addresses the complex ethical aspects associated with IVM and the questions of whether, and under what conditions, the production of IVM represents an ethically justifiable solution for existing problems, especially in view of animal welfare, the environment, and society. There are particular hopes regarding the benefits that IVM could bring to animal welfare and the environment, but there are also strong doubts about their ethical benefits.
Novel visual tracking approach via ant lion optimiser
Authors: Zhang Huanlong , Gao Zeng , Zhang Jie , Lu Xiankai , Chen Jian , Nie Guohao , Qian Xiaoliang
Published: November 2019

Ant lion optimiser (ALO) is a new nature‐inspired swarm intelligence optimisation algorithm that mimics the hunting mechanism of antlions in nature. ALO has been proved to have the merits of high exploitation and convergence speed benefiting from adaptive boundary shrinking mechanism and elitism. In this work, visual tracking is expressed as searching for object in whole search space by interaction between antlions and ants. A novel ALO‐based visual tracking framework is proposed and the adaptation and sensitivity of the parameters in ALO are discussed to improve tracking performance. In addition, considering that ALO tracker needs a lot of iteration consumption, kernel correlation filter with deep feature is integrated into the ALO tracking framework (ALOKCF) to improve track efficiency. Extensive experimental results prove that the ALO tracker is very competitive compared to other trackers, especially for abrupt motion tracking. At the same time, two visual tracking benchmarks are used to verify ALOKCF tracker achieves state‐of‐the‐art performance.
Late encounter events as source of disks and spiral structures
Authors: Kuffmeier M. , Goicovic F. G. , Dullemond C. P.
Published: November 2019

Context. Observations of arc-like structures and luminosity bursts of stars >1 Myr in age indicate that at least some stars undergo late infall events.

Aims. We investigate scenarios of replenishing the mass reservoir around a star via capturing and infalling events of cloudlets.

Methods. We carried out a total of 24 three-dimensional hydrodynamical simulations of cloudlet encounters with a Herbig star of mass 2.5 M⊙ using the moving-mesh code AREPO. To account for the two possibilities of a star or a cloudlet traveling through the interstellar medium (ISM), we put either the star or the cloudlet at rest with respect to the background gas.

Results. For absent cooling in the adiabatic runs, almost none of the cloudlet gas is captured as a result of high thermal pressure. However, second-generation disks easily form when accounting for cooling of the gas. The disk radii range from several 100 to ~1000 au and associated arc-like structures up to 104 au in length form around the star for runs with and without stellar irradiation. Consistent with angular momentum conservation, the arcs and disks are larger for larger impact parameters. Accounting for turbulence in the cloudlet only mildly changes the model outcome. In the case of the star being at rest with the background gas, the disk formation and mass replenishment process is more pronounced and the associated arc-shaped streamers are longer lived.

Conclusions. The results of our models confirm that late encounter events lead to the formation of transitional disks associated with arc-shaped structures such as observed for AB Aurigae or HD 100546. In addition, we find that second-generation disks and their associated filamentary arms are longer lived (>105 yr) in infall events, when the star is at rest with the background gas.
Temperature profiles of young disk-like structures
Authors: van ’t Hoff Merel L. R. , van Dishoeck Ewine F. , Jørgensen Jes K. , Calcutt Hannah
Published: November 2019

Context. Temperature is a crucial parameter in circumstellar disk evolution and planet formation because it governs the resistance of the gas to gravitational instability and sets the chemical composition of the planet-forming material.

Aims. We set out to determine the gas temperature of the young disk-like structure around the Class 0 protostar IRAS 16293–2422A.

Methods. We used Atacama Large Millimeter/submillimeter Array (ALMA) observations of multiple H2CS J = 7 − 6 and J = 10 − 9 lines from the Protostellar Interferometric Line Survey (PILS) to create a temperature map for the inner ~200 AU of the disk-like structure. This molecule is a particularly useful temperature probe because transitions between energy levels with different Ka quantum numbers operate only through collisions.

Results. Based on the H2CS line ratios, the temperature is between ~100–175 K in the inner ~150 AU, and drops to ~75 K at ~200 AU. At the current resolution (0.5′′~70 AU), no jump is seen in the temperature at the disk–envelope interface.

Conclusions. The temperature structure derived from H2CS is consistent with envelope temperature profiles that constrain the temperature from 1000 AU scales down to ~100 AU, but does not follow the temperature rise seen in these profiles at smaller radii. Higher angular resolution observations of optically thin temperature tracers are needed to establish whether cooling by gas-phase water, the presence of a putative disk, or the dust optical depth influences the gas temperature at ≲100 AU scales. The temperature at 100 AU is higher in IRAS 16293A than in the embedded Class 0/I disk L1527, consistent with the higher luminosity of the former.
Long-term changes in the diversity, abundance and size composition of deep sea demersal teleosts from the Azores assessed through surveys and commercial landings
Authors: Santos Régis V.S. , Silva Wendell M.M.L. , Novoa-Pabon Ana M. , Silva Hélder M. , Pinho Mario R.
Published: November 2019

To obtain important baseline information about population dynamics and to provide valuable insights about the possible effects of artisanal fishing on the demersal fish community, this study had three specific objectives: (1) to update the annotated list of demersal teleost species occurring in the Azores (mid-North Atlantic); (2) to describe their depth distribution and related fishery information; and (3) to evaluate annual changes in their observed abundance and length composition. To do this, a time series with about 25 years of scientific data from the commercial fishery and surveys was analyzed. The demersal teleost community was represented by 213 species, predominantly deep-water species, reflecting the main marine habitat in the Azores region. Fishery takes place mainly in the intermediate strata (200–600 m), where the most commercially important species occur. No changing in the fork length (LF) distribution toward small individuals caused by size-selective fishing was detected in this study. The high variability observed in theLFdata indicates the need for more accurate studies considering alternative statistical analyses (e.g., generalised linear models) to examine the possible factors (e.g. depth coverage, gear configuration, soak time, and fishing area) that cause this variability. On the other hand, the abundance indices of some commercially important species appear to have declined (Phycis phycis,Pontinus kuhlii,Conger conger,Beryx splendensandB. decadactylus), while others appear to be more stable (Helicolenus dactylopterus) or even increasing (Mora moro). Although the available data are insufficient for a formal assessment on the status of exploited populations, the long-term analysis of commercial landings and survey data was used as a basis to assess deep sea demersal teleost fisheries of the Azores, under a precautionary approach.
The Advances and Challenges of CAR-NK Cells for Tumor Immunotherapy
Authors: Ye Ziyun A.
Published: November 2019

Immunotherapies using chimeric antigen receptor (CAR)-T cells bring an encouraging vision to non-Hodgkin lymphoma patients who develop relapsed lymphoma or are unresponsive to standard chemotherapy, yet they also have limitations and drawbacks. Clinical trials have reported cases of neurotoxicity and cytokine release syndrome (CRS) accompanied by CAR-T cell therapies. To establish a more mature therapy, CAR incorporated into Natural Killer (NK) cells came into being. As a leukocyte involved in innate immunity, NK cell does not require MHC matching, making the production of allogeneic “off-the-shelf” CAR-NK cells possible. Moreover, the controllable life span of CAR-NK cells and little risk of graft-versus-host disease reduce side effects companion by CAR-T. This review provides an overview of CAR-NK design and production before delivery to patients. Different sources of NK cells are compared and the development of CAR molecule construction is introduced.
Chitosan composites nerve conduits
Authors: Chen Yifan
Published: November 2019

Traumatic nerve defect affected over 200, 00 people in the US. As it affected so many people, autografts as the golden standard solution have their limitations. Nerve conduits is a new method and the materials for conduits establishing contain biological materials, synthetic materials and composite materials. Among these categories, composite conduits appear better regeneration ability. Also, chitosan demonstrated have the ability to help nerve regeneration. Thus, chitosan composite may be a good choice in the future. This article compares six kinds of chitosan composite: Laminin-chitosan-PLGA, Chitosan-Zinc Oxide Nanocomposite, PCL/Chitosan/Verapamil, Chitosan/ hyaluronic acid conduit, Chitosan/simvastatin/Pluronic F-127 hydrogel and Chitin/ Carboxymethyl (CM)-chitosan by conduit structure, establishment method, sciatic functional index (SFI), electrophysiology and histology data. This article concludes that chitosan-drug-drug delivery system showed the best recovery result.
The Mechanism of Fear at Behavior, Brain Circuits and Molecular Level
Authors: Chen Yuting
Published: November 2019

The study of “fear” can be traced back to the differences between “behaviorism” and “psychoanalysis” on cognition, while recent research on the learning and memory of fear by using modern cell and molecular biology experimental techniques has made our understanding of “fear” gradually multi-level and in-depth. At present, our research on fear can be divided into three levels, namely behavior, brain circuits and molecular. At the level of behavior, Pavlovian conditioning experiments have become the core method for studying fear, and behavioral approaches have been used for fear-related mental illnesses, such as the treatment of Post-Traumatic Stress Disorder (PTSD). At the level of brain circuits, the brain circuits with the amygdala as the core have gradually become clear, and there is also a two-system or one-system debate on how to recognize the fear process. At the molecular level, the Hebbian synaptic plasticity mechanism and neuromodulatory-dependent mechanism are used to explain the process of fear memory acquisition. Based on the current three levels of research, revealing how feelings of fear may generate is likely to play a guiding role in the further understanding of “fear” and treatment of related diseases.
Constructing and analyzing a disease network based on proteins
Authors: Li Ying , Huang Wencong
Published: November 2019

Protein is the specific executor of life activities, but there is no protein-based disease network and the current disease networks cannot show that a disease group share the same factors. We propose a method to construct a protein-based network by assigning disease pairs to different intervals according to their similarities and searching for disease groups in each interval. Statistical methods are used to analyze the disease network, and the result indicates that : in the case where a disease belongs to only one disease group, most diseases have their own protein characteristics, but the common protein of them is not obvious; the more diseases a protein is related to, the more likely the protein becomes common protein; diseases grouping at protein level in this study are different from traditional disease classification; there is a certain relationship between disease symptoms and underlying proteins, but not one-to-one correspondence.
Biosynthesis of Red Elemental Selenium Protein
Authors: Wu Guojie , Lin Xuegui , Liu Yong , Peng Jinwen , Huang Jinhui
Published: November 2019

The red elemental selenoprotein was biosynthesized by active dry yeast in this study. Through a single factor experiment and L9(34)-orthogonal test fermentation condition optimization, the selenium content was 15.72 mg/g under optimal condition. The optimum culture condition was as follows: the sodium selenite concentration (0.8 mg/mL), culture time (36 h), the yeast dosage (5 g), pH (5.5), culture temperature (30°C), and the medium Baume degree (8 °Bé). The analysis of amino acid species, content and morphology of the product showed that the red elemental selenoprotein was successfully synthesized.
Resolving Trypanosoma brucei Flagellar Structure by Cryo-Electron Tomography
Authors: Dai Jennifer
Published: November 2019

Trypanosoma brucei is a unicellular eukaryote that can cause human African trypanosomiasis, which has continued to evolve and spread. The key feature of these parasites is that they have a flagellum consists of a typical 9 + 2 axoneme and a lattice-like paraflagellar rod (PFR). It attached to the cell body and is responsible for cell motility, cytokinesis, and morphogenesis. The present study demonstrates the detailed structure and defines the length of the axoneme and three domains of the paraflagellar rod (PFR) using cryo-electron tomography of Trypanosoma brucei flagella. The performed analysis revealed the double-headed structure of the outer-arm dynein, the internal structure of PFR and identified repeating structure in the flagella. Since these structures are critical to the pathogenicity of Trypanosoma brucei, and understanding their organization would help in finding treatments against African trypanosomiasis.
The development of sports rehabilitation industry and strategy in China under the vision of great health
Authors: Sun Xiaoyu
Published: November 2019

With the development of national economy and the improvement of people’s quality of life, the concept of sports rehabilitation has gradually been deeply rooted in the hearts of people, which has brought about the rapid development of sports rehabilitation industry. However, at present, sports rehabilitation industry in China is still in its early stage and there are many problems and contradictions that should be focused on in the process of development .This paper mainly analyzes and discusses the problems arising in the development of the sports rehabilitation industry, and puts forward the reform measures based on the current problems, aiming to provide reference for the future development of the sports rehabilitation industry.
Study on anti-tumor activity of itraconazole in colon cancer
Authors: Li Xiaoqi , Shan Jingbo , Jin Lijun , Li Xinyu , Zhou Sa , Ma Wenjian
Published: November 2019

Itraconazole is a common antifungal drug, which inhibiting lanosterol 14 alpha-demethylase, interfering with lanosterol convert to ergosterol or cholesterol, thereby affecting the synthesis of fungal cell membranes. In recent years, it has been found that it has anti-cancer effect and is expected to be used to treat a variety of human cancers. Colon cancer is a cancer disease that affects the health of contemporary people and its incidence tends to be younger. In this paper, the effects of itraconazole on proliferation and migration of Caco2 and HT-29 of two kinds of colon cancer cells were detected by MTT, scratch experiment and western blot. The data showed that itraconazole concomitant with increased drug concentration caused cell morphology of colon cancer significantly shrink. MTT experiment showed that it could reduce the survival rate of colon cells and western blot data indicated that it could down-regulation PCNA and MYL9 which are marker genes of proliferation and migration. Furthermore, the cell migration is reduced.
Analysis and expression of Pmlyzi3 from Penaeus monodon
Authors: Wang Chaogang , Li Guoqiang , Zhou Liang , Li Anguo , Shang Chenjing , Deng Xu
Published: November 2019

Lysozymes are crucial immune moleculars and play an important role in innate imunity. Here, a new lysozyme named Pmlyzi3 was found from the transcriptome data of Panaeus monodon. The Pmplyzi3 gene was 438bp in length, encoding a 146-residues peptide and the first 19 residues constituted a signal peptide. The mature peptide contained 10 cysteines and had 7 α-helixes in its N terminal. Moreover, it showed 88% identity with lysozyme-like protein from Penaeus vannamei. To express Pmlyzi3, pColdIV-SUMOPmlyzi3 plasmid was constructed by linked the Pmlyzi3 with SUMO tag, then transformed to Eschericha coli BL21 (DE3). By optimizing expression condition, SUMO-Pmlyzi3 was succeeded in expression in high level and purifing with Ni-NTA column. Following with SUMO protease excision, pure Pmlyzi3 was obtained by removing SUMO tag, which would be helped to study its function.
Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection
Authors: Niu Lili , Liu Hongli
Published: November 2019

To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages. Then, the parasites are detected to observe the application characteristics of the RPA method. The results show that RPA is a kind of novel isothermal nucleic acid amplification technology, which is an open detection method. It has high sensitivity and specificity when being operated at 37-42°C, which makes it very suitable for early detection of pathogen infection. Besides, it also has high sensitivity and specificity in parasite detection. Therefore, the RPA technology has better performances and excellent applications in parasite detection, which has a certain significance for the future application of the technology in more fields.
Sequence stratigraphy of the Cambrian Terreneuvian Dalinzi Formation in southern Liaoning
Authors: Wu Zijie , Zhong Mishan , Gao Fuliang , Luo Niangang
Published: November 2019

As one of the only Cambrian Terreneuvian rock stratas in southern Liaoning, the Dalinzi Formation is of great significance for studying the evolution of the Early Cambrian sedimentary basin and the global sea level change in North China. Taking the Zhaokanzi section as an example, the sedimentary facies of the Dalinzi Formation in southern Liaoning were identified, and then the sequence stratigraphy was studied. The study suggests that the Dalinzi Formation is formed in the lagoon and tidal flat sedimentary environment, and a three-level sequence and several parasequences and parasequence sets can be divided. The relative sea level changes and sedimentary basin evolution are discussed.
Comprehensive Study of Metamorphic Core Complex in Southern Liaodong Peninsula, NE China
Authors: Zhong Mishan , Wu Zijie , Liu Jin , Zhang Guoren , Wang Qi , Gao Fuliang , Pan Yuqi , Gao Yongzhao
Published: November 2019

Through this work and comprehensive research on a large amount of previous data, four metamorphic core complexes were identified in the south of Liaodong peninsula, which were respectively Jinzhou metamorphic core complex, Wanfu metamorphic core complex, Xinfang metamorphic core complex and Lizifang metamorphic core complex. Xinfang metamorphic core complex and Lizifang metamorphic core complex were discovered and proposed for the first time. They have kinematic unity, geometric asymmetry, tectonic magma joint activity and the difference in formation time.
Sequence Stratigraphy towards its standardization—an important scientific scheme
Authors: Wu Heyuan , Khan Muneeb , Song Ping
Published: November 2019

In the Post-Exxon Era of sequence stratigraphy, various sequence models for the complex stratigraphic records with their response mechanisms are developed. All the models with strong pertinence are endowed, which lead to misapprehension in the conceptual system. Therefore, the standardization of sequence stratigraphy with the aim to provide consistency in the terminology has become an important motive of modern sequence. During the development of sequence stratigraphy, the identification and distinction between normal and forced regression have laid important foundation for the system description of sequence development. This becomes the first step towards the standardization because of model-independent nature. The introduction of model-independent unconventional system tracts in fluvial sequence models, which are low- and high-accommodation system tracts, which turn out to be another successful attempt of towards the standardization of sequence stratigraphy. The four parts of stratigraphic records, which include the complexity and cyclicity in the stratigraphic accumulation process; the non-gradual change and the non-integrity of the stratigraphic records; the variability represented by the diversity of the sequence models and the nature of standardization including variability, will provide more clues and approaches for further sequence stratigraphy development
Protection and enlightenment of ecological integrity of Canadian national parks
Authors: Yang Shuhui , Duan Xiaoyu
Published: November 2019

Canada is one of the first countries in the world to establish a national park, and pioneered the concept of ecological integrity management of national parks. Based on this concept, the country has basically achieved the sustainable development of national parks. China has a vast territory, a large number of scenic spots and nature reserves, but its system and management methods need to be optimized. This paper takes forestry developed countries as an example, summarizes the progress of ecological integrity protection in Canadian national parks, summarizes its current ecosystem adaptive management concepts and implementation methods, Ecological Integrity (EI) monitoring construction and related evaluation index systems, ecosystem protection and restoration. The experience is intended to provide a reference for the improvement of the ecological integrity protection of national parks in China.
A method for detecting and locating the gross error of tidal based on wavelet analysis
Authors: Zhang Jin , Li ShuJun , Meng FanJun
Published: November 2019

Tidal harmonic constants are necessary data for the evaluation of tidal model, tidal prediction and chart datum. Compared with the wavelet multi-scale analysis model, a new method is put forward to detect and locate tidal discrete and continuous gross error. Based on the properties of the wavelets, the wavelet suitable for detecting the gross error of tidal is selected. And taking db6 wavelet as an example, the feasibility and effectiveness of this method are proved by experiments. The results show that the method can not only simultaneously detect and locate discrete gross error and continuous gross based on high frequency information, and can detect and locate the systematic deviation caused by the zero drift according to the low frequency information. Experimental result shows that the method is more simple, the efficiency and accuracy of detecting and locating gross error are improved.
The Passive Surface Wave Methods for Shallow Engineering Exploration Based on the ESPAC Technology
Authors: Wu Tong , Song Kezhu , Sun Zhengyang , Zhao Hongwei , Hu Xin
Published: November 2019

ESPAC method is a rapidly emerging field of seismological research, which can reflect the physical properties of the Earth’s medium. In the process of using the ESPAC method, sometimes the noise of the original data is relatively large, and the raw data of each seismometer needs to be preprocessed, including operations such as de-averaging, de-trending, re-sampling, normalization, and filtering. The selection of the normalized method and the selection of the bandwidth of the filter are particularly important, and it will produce the wrong result if not handled properly. This article attempts to use the extended spatial autocorrelation (ESPAC) method to extract Rayleigh-wave phase velocity dispersion curves from the vertical component of the seismic stations’ microtremors, and proposes feasible and effective solutions to the selection of the normalized method and bandwidth of bandpass filtering.
Research on Accuracy of Normal Moveout in Reflection Seismic Exploration by Cross Correlation Method
Authors: Huang Yan , Zhou Hefeng , Zeng Xinwu
Published: November 2019

In this paper, we took the time-distance curve equation and the Normal Moveout (NMO) principle as breakthrough point. According to the forward seismic simulation model and the cross-correlation principle in signal processing, we have convincingly studied the accuracy of NMO in simulation experiment. Firstly, the time-distance curve equation of single-layer reflection and the characteristics of cross-correlation are simply analysed. Then, we build the seismic forward model by Tesseral software, and accurate results are obtained after the simulation experiment is carried out. Finally, we get the conclusion by analysing and summarizing these results. The conclusion shows that the method effectively demonstrates the feasibility of the cross-correlation method in testing the accuracy of NMO, and provides a new idea for analysing the accuracy of velocity picking, even the accuracy of the underground model during petroleum exploration.
The Application of Magnetometers and Electromagnetic Induction Sensors in UXO Detection
Authors: Han Songtong , Rong Xiaoli , Bian Leixiang , Zhong Mingyou , Zhang Lining
Published: November 2019

This paper introduces the physical model of unexploded ordnance (UXO) in the magnetization process and the formula derivation of the magnetic dipole model. The application of magnetometers and electromagnetic induction sensors in the detection of UXO is introduced. The magnetometers introduce the total field measurements technology of the CS optical-pump marine magnetometer and the MagSTAR (Magnetic Scalar Triangulation and Ranging) gradient detection technology. The electromagnetic induction sensors introduce the working theories and the popular product models of Geophex company and Geonics Ltd. The method of detecting UXO by MTADS of United States Naval Research Laboratory (The multisensor towed array detection system) compares the difference between the United States Naval Research Laboratory for target recognition.
Difference Analysis of Ecological Security Assessment Based on FAHP
Authors: Yin Yahao , Li Haihong , An Fengqiu
Published: November 2019

In this study, the important city of Guanzhong Plain-Weinan, was studied and its ecological security index changed from the year of 2000 to 2012. The stress-state-response (PSR) model was used to establish an ecological security assessment system based on agricultural development cities. The method of quantitative research including 12 indicators based on the fuzzy analytic hierarchy process (FAHP) model was constructed, and the main aspects such as the natural environment, economic society, and human activities were designed. The results showed that from 2000 to 2012, the area of ecological security and stability is gradually increasing, and the stability of the ecological environment is enhanced. The ecological security level in the central region of the country has developed well in the past 10 years, but the ecological security level in some areas has gradually increased in edge area.
Application Of Comprehensive Evaluation Method In Oil Well Classification
Authors: Huang Fada , Tang Yifang , Huang Liang , Zhang Haitao
Published: November 2019

China’s oil resources are very rich, but the effect of oil exploitation is not good. Precise prediction of oil production plays an extremely important role in formulating a reasonable oil planning plan and determining the direction of investment. In order to improve the accuracy of oil well exploitation, it is necessary to establish a scientific oil production forecasting system. First, pre-treatment research on the data of Baikouquan Oilfield to ensure the quality of oilfield data. Secondly, the main controlling factors affecting the production of oil wells are analyzed, and the single evaluation is carried out by the coefficient of variation method, the entropy method and the mean square error method. Then, the pre-examination test of the consistency test is performed on the single evaluation result, and the combination of the evaluation methods is selected. The combined evaluation method is the average method, and the fuzzy Borda method. It is again tested whether the combined evaluation result has passed the consistency test. After the consistency test, the sum of the squares of the errors of the three combined evaluations is calculated, and the optimal one is optimized. The combined evaluation method was used for the second combination evaluation, and the results of all the evaluation methods were compared to observe whether the combined evaluation results were effective, and 8 main control factors were analyzed and screened. Finally, the main control factor data analyzed by comprehensive evaluation is used for discriminant analysis, and the fitting effect is compared. It is found that the fitting results are basically consistent with the data of the oilfield site, indicating that the results of the main control factors analyzed by the comprehensive evaluation are better. The prediction accuracy meets the engineering requirements and is of great significance for the prediction of on-site oilfield production.
Application and Research of Geologic Radar in Groundwater Disease of Tunnel Engineering Inspection
Authors: Wang Yu , Wu chuang-sheng , Shu Zongying
Published: November 2019

As a kind of underground lineament building, the tunnel inevitably will pass through different hydrology geology in construction process and thus form a catchment corridor which usually occurs in most of tunnels. Pour water and waterlogging is a common geological disaster and the main disease both in the construction tunnels and operation tunnels. So it is urgent for us to carry through the research on the geological disaster of groundwater. The Inspecting and controlling measures against the ground water geological disaster are also needed. In this paper, Geologic Radar is applied to tunnel engineering quality inspection to detect some quality problems possibly caused by groundwater. In order to diagnose the disease degree of operation tunnels and to estimate tunnel’s security condition, this paper analyzes the causes of bugs existing behind the tunnel lining, expounds the theory and method of making non-destructive test on tunnel lining quality by using Geological Radar. By processing and analyzing field data, the following functions can be performed, such as detecting the thickness of concrete lining accurately, determining the distribution position and quantities of rebar and grid steel, searching existed cavity and uncompacted area behind the lining especially lining arch top. We also apply the method of morphology to the extraction of liner image feature. We get the liner boundary line and waterlogging area more intuitively by wavelet analysis, and find the anomalous point in the liner. It has been proved that Geologic Radar is a feasible method to inspect the structure of tunnel concrete lining. Adopting hole-drilling method to make verifications with detecting results of geological radar, the results of the two kinds of methods do not have big difference.
The Impact of Environmental Protection Tax on the Upgrading of Industrial Structure-Based on Spatial Econometric Analysis
Authors: Guan Aiping , Xie Jing , Meng Yu
Published: November 2019

Based on the provincial panel data of 30 provinces in China from 2000 to 2017, this paper uses the spatial econometric model to analyze the impact of environmental protection tax on the upgrading of industrial structure under three spatial weight matrices. The results show that there are significant positive spatial correlations in the upgrading of provincial industrial structure in China, and environmental protection tax has a significant positive spatial spillover effects on the upgrading of industrial structure. The implementation of environmental protection tax in provinces and regions will promote the upgrading of local industrial structure, which will have a positive impact on the upgrading of industrial structure in neighboring provinces and regions.
Sustainable management of irrigation infrastructures in China: The role of lineage and resource endowment
Authors: Yang Yu , Wang Fang
Published: November 2019

From perspectives of the degree of lineage networks, households’ residence in this paper we analyzes the influence of the lineage networks and the resource endowment on the households’ participation willingness to contribute to irrigation infrastructures. With the fieldwork data in Jintang County of Chengdu City, we found that the degree lineage networks is helpful to enhance the belonging sense of households, so as to promote the households’ willingness. Living in cities and towns will weaken households’ participation willingness to contribute to small irrigation infrastructures. Hakka households’ participation willingness to contribute to small irrigation infrastructures prefer the interests of their own lineages. Our research helps to understand how lineage informal institutions facilitate sustainable management of irrigation infrastructures.
Impervious surface extraction of multi-source high-resolution images in 2018 in Anhui Province
Authors: Wang Fan , Jia Xiaofeng , Li Mengya , Jia Shijie
Published: November 2019

With the development of the economy and the continuous improvement of social demand, the impervious area is more and more representative of the urbanization process and economic development level of the society. Anhui Province is a big province, and the impervious information is an essential element for an accurate understanding of economic development. To accurately understand the impervious surface and economic development level of Anhui Province, this study selects the training samples of Anhui province, trains the classifier, uses the classification algorithm of support vector machine, combines 2018 Sentinel-2 and Landsat8 in Matlab, and uses the LUOJIA-1 nighttime light data as auxiliary. The data was used to make land use planning maps for forests, farmland, impervious surfaces and water bodies in Anhui Province, and then the impervious information of the province in Anhui Province was extracted with high precision in 2018. Compared with the results of Sentinel-2&LUOJIA-1 and Landsat8&LUOJIA-1, it is proved that the combination of Sentinel-2, Landsat8 and LUOJIA-1 data can extract impervious information with high accuracy and high precision.
The Modification of Difference Vegetation Index (DVI) in middle and late growing period of winter wheat and its application in soil moisture inversion
Authors: Hong-wei Zhang , Chen Huai-liang , Zha Fei-na
Published: November 2019

In the middle and late growing period of winter wheat, soil moisture is easily affected by saturation when using MODIS data to retrieve soil moisture. In this paper, in order to reduce the effect of the saturation caused by increasing vegetation coverage in middle and late stage of winter wheat, the Difference Vegetation Index (DVI) model was modified with different coefficients in different growth stages of winter wheat based on MODIS spectral data and LAI characteristics of variation. LAI was divided into three stages, LAI ≤ 1 < LAI ≤, 3 < LAI, and the adjusting coefficient of α=1, α=3, α=5, were taken to modifying the Difference Vegetation Index(DVI). The results show that the Modified Difference Vegetation Index (MDVIα) can effectively reduce the interference of saturation, and the inversion result of soil moisture in the middle and late period of winter wheat growth is obviously superior to the uncorrected inversion model of DVI.