bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Genomic Hybridization and Transcriptome Sequencing Reveal Genes with Gain in Acute Lymphoblastic Leukemia: JUP Expression Emerges as a Survival-Related Gene
Authors: Zapata-García Jessica Alejandra , Riveros-Magaña Alma Rocío , Ortiz-Lazareno Pablo Cesar , Hernández-Flores Georgina , Jave-Suárez Luis Felipe , Aguilar-Lemarroy Adriana
Published: November 2022

Acute lymphoblastic leukemia (ALL) in children or adults is characterized by structural and numeric aberrations in chromosomes; these anomalies strongly correlate with prognosis and clinical outcome. Therefore, this work aimed to identify the genes present in chromosomal gain regions found more frequently in patients with acute lymphoblastic leukemia (ALL) and ALL-derived cell lines using comparative genomic hybridization (CGH). In addition, validation of the genes found in these regions was performed utilizing RNAseq from JURKAT, CEM, and SUP-B15 cell lines, as well as expression microarrays derived from a MILE study. Chromosomes with common gain zones that were maintained in six or more samples were 14, 17, and 22, in which a total of 22 genes were identified. From them, NT5C3B, CNP, ACLY, and GNB1L maintained overexpression at the mRNA level in the cell lines and in patients with ALL. It is noteworthy that SALL2 showed very high expression in T-ALL, while JUP was highly expressed in B-ALL lineages. Interestingly, the latter correlated with worse survival in patients. This provided evidence that the measurement of these genes has high potential for clinical utility; however, their expressions should first be evaluated with a sensitive test in a more significant number of patients.
Macrophage-like Cells Are Increased in Patients with Vision-Threatening Diabetic Retinopathy and Correlate with Macular Edema
Authors: Zhang Nigel T. , Nesper Peter L. , Ong Janice X. , Wang Jacob M. , Fawzi Amani A. , Lavine Jeremy A.
Published: November 2022

Macrophage-like cells (MLCs) are potential inflammatory biomarkers. We previously showed that MLCs are increased in proliferative diabetic retinopathy (PDR) eyes. Vision-threatening diabetic retinopathy (VTDR) includes PDR, severe non-PDR (NPDR), and diabetic macular edema (DME). No prior data exist on MLCs in eyes with severe NPDR or DME. This prospective, cross-sectional optical coherence tomography-angiography (OCT-A) imaging study included 40 eyes of 37 participants who had NPDR classified as non-VTDR (n = 18) or VTDR (n = 22). Repeated OCT-A images were registered, averaged, and used to quantify the main outcome measures: MLC density and percent area. MLC density and percent area were correlated with clinical characteristics, NPDR stage, presence of DME, and OCT central subfield thickness (CST). In VTDR eyes, MLC density (2.6-fold, p < 0.001) and MLC percent area (2.5-fold, p < 0.01) were increased compared with non-VTDR eyes. Multiple linear regression analysis between MLC metrics and clinical characteristics found that MLC density was positively correlated with worse NPDR severity (p = 0.023) and higher CST values (p = 0.010), while MLC percent area was only positively associated with increased CST values (p = 0.006). MLCs are increased in patients with VTDR. Macular edema is the most strongly associated factor with increased MLC numbers in NPDR eyes.
Video Action Recognition Using Motion and Multi-View Excitation with Temporal Aggregation
Authors: Joefrie Yuri Yudhaswana , Aono Masaki
Published: November 2022

Spatiotemporal and motion feature representations are the key to video action recognition. Typical previous approaches are to utilize 3D CNNs to cope with both spatial and temporal features, but they suffer from huge computations. Other approaches are to utilize (1+2)D CNNs to learn spatial and temporal features in an efficient way, but they neglect the importance of motion representations. To overcome problems with previous approaches, we propose a novel block which makes it possible to alleviate the aforementioned problems, since our block can capture spatial and temporal features more faithfully and efficiently learn motion features. This proposed block includes Motion Excitation (ME), Multi-view Excitation (MvE), and Densely Connected Temporal Aggregation (DCTA). The purpose of ME is to encode feature-level frame differences; MvE is designed to enrich spatiotemporal features with multiple view representations adaptively; and DCTA is to model long-range temporal dependencies. We inject the proposed building block, which we refer to as the META block (or simply “META”), into 2D ResNet-50. Through extensive experiments, we demonstrate that our proposed method architecture outperforms previous CNN-based methods in terms of “Val Top-1 %” measure with Something-Something v1 and Jester datasets, while the META yielded competitive results with the Moment-in-Time Mini dataset.
Online Tracking of Maneuvering Target Trajectory Based on Chaotic Time Series Prediction
Authors: Wei Qian , Su Peng , Zhou Lin , Shi Wentao
Published: November 2022

Online prediction of maneuvering target trajectory is one of the most popular research directions at present. Specifically, the primary factors balancing, between prediction accuracy and response time, will give the research substance. This paper presents an online trajectory prediction algorithm based on small sample chaotic time series (OTP-SSCT). First, we optimize in terms of data breadth. The dynamic split window is built according to the motion characteristics of the maneuvering target, thus realizing trajectory segmentation and constructing a small sample chaotic time series prediction set. Second, since fully considering the motion patterns of maneuvering targets, we introduce the spatiotemporal features into the particle swarm optimization (PSO) model identification algorithm, which improves the identification sensitivity of key trajectory data points. Furthermore, we propose a feedback optimization strategy of residual compensation to correct the trajectory prediction values to improve the prediction accuracy. For the initial value sensitivity problem of the PSO model identification algorithm, we propose a new initial population strategy, which improves the effectiveness of initial parameters on model identification. Through simulation experiment analysis, it is verified that the proposed OTP-SSCT algorithm achieves better prediction accuracy and faster response time.
Colorectal Cancer Survival Prediction Using Deep Distribution Based Multiple-Instance Learning
Authors: Li Xingyu , Jonnagaddala Jitendra , Cen Min , Zhang Hong , Xu Steven
Published: November 2022

Most deep-learning algorithms that use Hematoxylin- and Eosin-stained whole slide images (WSIs) to predict cancer survival incorporate image patches either with the highest scores or a combination of both the highest and lowest scores. In this study, we hypothesize that incorporating wholistic patch information can predict colorectal cancer (CRC) cancer survival more accurately. As such, we developed a distribution-based multiple-instance survival learning algorithm (DeepDisMISL) to validate this hypothesis on two large international CRC WSIs datasets called MCO CRC and TCGA COAD-READ. Our results suggest that combining patches that are scored based on percentile distributions together with the patches that are scored as highest and lowest drastically improves the performance of CRC survival prediction. Including multiple neighborhood instances around each selected distribution location (e.g., percentiles) could further improve the prediction. DeepDisMISL demonstrated superior predictive ability compared to other recently published, state-of-the-art algorithms. Furthermore, DeepDisMISL is interpretable and can assist clinicians in understanding the relationship between cancer morphological phenotypes and a patient’s cancer survival risk.
Internationalisation of Teaching and Learning through Blended Mobility: Potentials of Joint International Blended Courses and Challenges in Their Implementation
Authors: Perfölz René , López-Varela Asun
Published: November 2022

Blended Mobility formats such as joint international blended courses have the potential to enable more students at universities and other HEIs to gain international experiences in the course of their studies. They enhance transnational cooperation in the European Higher Education Area by building bridges at the crossroads of education, research, innovation, serving society and economy. In this article, the authors reflect on their experiences in the conception, planning, organisation and implementation of a joint international blended course between Freie Universität Berlin and Universidad Complutense de Madrid in the field of sustainable development in the summer semester of 2022. The course was offered within the framework of the Erasmus+ KA3 project “Online Pedagogical Resources for European Universities” (OpenU project).
Simplex Lattice Design and X-ray Diffraction for Analysis of Soil Structure: A Case of Cement-Stabilised Compacted Tills Reinforced with Steel Slag and Slaked Lime
Authors: Lindh Per , Lemenkova Polina
Published: November 2022

Evaluating the structure of soil prior to building construction is valuable in a large variety of geotechnical and civil engineering applications. To built an effective framework for assessing the strength of the stabilised soil, the presented workflow includes a complex approach of simplex lattice design and X-ray diffraction for the analysis of soil structure. Different from the traditional in situ measurements, we propose a statistical framework for effective decision-making on binder combination to stabilise soil collected in three localities of Southern Sweden—Bromölla Municipality (Skåne County), Petersborg (Östergötland County) and Örebro (Örebro County). A practical solution is presented that includes the evaluation of strength properties of various types of soil using ordinary Portland cement (OPC), slaked lime and steel slag as pure agents and blended binders. The specimens were collected in Southern Sweden and included sandy silty tills and clay till (clay content 6–18%). The preprocessing included the mineralogical analysis of mineral composition and soil structure by X-ray diffraction (XRD) and a sieve. The soil samples were fabricated, compacted, rammed, stabilised by six binder blends and assessed for uniaxial compressive strength (UCS). The moisture condition value (MCV) and water content tests were done for compacted soil and showed variation in the MCV values for different binders. The study determined the effects from binder blends on the UCS gain in three types of soil, measured on days 7, 28 and 90. Positive effects were noted from the steel slag/lime blends on the UCS gain in sandy silty tills. A steel slag/slaked lime mixed binder performed better compared to the pure binders. The effectiveness of the simplex lattice design was demonstrated in a series of ternary diagrams showing soil strength evaluated by adding the stabilising agents in different proportions.
Thriving or Surviving in the Energy Industry: Lessons on Energy Production from the European Economies
Authors: Batrancea Larissa M. , Tulai Horia
Published: November 2022

The energy industry has a major impact on the sustainability level of global economies, from job creation to resource efficiency and environmental impact. The aim of our study was to examine the phenomenon of energy production and its main determinants in 37 European economies during the period of 2011–2021. The methodological approach entailed the use of the panel first-difference generalized method of moments (GMM). The empirical results show that the relationship between energy production and predictors such as imports, energy prices, energy productivity, and energy efficiency was monotonic. At the same time, our results indicate a relationship between energy supply and energy production, imports, productivity, and prices. Moreover, energy production depends significantly on the energy productivity, primary energy consumption, and energy imports.
Atmospheric Drivers of Wind Turbine Blade Leading Edge Erosion: Review and Recommendations for Future Research
Authors: Pryor Sara C. , Barthelmie Rebecca J. , Cadence Jeremy , Dellwik Ebba , Hasager Charlotte B. , Kral Stephan T. , Reuder Joachim , Rodgers Marianne , Veraart Marijn
Published: November 2022

Leading edge erosion (LEE) of wind turbine blades causes decreased aerodynamic performance leading to lower power production and revenue and increased operations and maintenance costs. LEE is caused primarily by materials stresses when hydrometeors (rain and hail) impact on rotating blades. The kinetic energy transferred by these impacts is a function of the precipitation intensity, droplet size distributions (DSD), hydrometeor phase and the wind turbine rotational speed which in turn depends on the wind speed at hub-height. Hence, there is a need to better understand the hydrometeor properties and the joint probability distributions of precipitation and wind speeds at prospective and operating wind farms in order to quantify the potential for LEE and the financial efficacy of LEE mitigation measures. However, there are relatively few observational datasets of hydrometeor DSD available for such locations. Here, we analyze six observational datasets from spatially dispersed locations and compare them with existing literature and assumed DSD used in laboratory experiments of material fatigue. We show that the so-called Best DSD being recommended for use in whirling arm experiments does not represent the observational data. Neither does the Marshall Palmer approximation. We also use these data to derive and compare joint probability distributions of drivers of LEE; precipitation intensity (and phase) and wind speed. We further review and summarize observational metrologies for hydrometeor DSD, provide information regarding measurement uncertainty in the parameters of critical importance to kinetic energy transfer and closure of data sets from different instruments. A series of recommendations are made about research needed to evolve towards the required fidelity for a priori estimates of LEE potential.
Peak Shaving in District Heating Utilizing Adaptive Predictive Control
Authors: Svensen Jan Lorenz
Published: November 2022

District heating systems (DHS) are driven by the heat demands of their consumers, with higher demands giving a higher load on the heat production. While heat demands are human-dependent, they contain diurnal behaviors and weather dependencies. The diurnal behaviors contain periods with high demands causing peak loads on the heat production, which is operationally costly. This is especially true for heat pumps, a solution for DHS to include green energy, as the cost depends directly on the needed temperature. This paper presents a formulation of adaptive model predictive control (MPC) for inducing peak shaving on the production load to handle the peak load problem by using the DHS distribution network as a heat storage. It also presents a simulator model to describe the DHS. The MPC was applied to data from a case study of the DHS in Brønderslev, Denmark, showing a peak reduction of around 8%.
Chemical Looping Reforming with Perovskite-Based Catalysts for Thermochemical Energy Storage
Authors: Padula Stefano , Tregambi Claudio , Troiano Maurizio , Di Benedetto Almerinda , Salatino Piero , Landi Gianluca , Solimene Roberto
Published: November 2022

The performance of a perovskite-based oxygen carrier for the partial oxidation of methane in thermochemical energy storage applications has been investigated. A synthetic perovskite with formula La0.6Sr0.4FeO3 has been scrutinized for Chemical Looping Reforming (CLR) of CH4 under fixed-bed and fluidized-bed conditions. Temperature-programmed reduction and oxidation steps were carried out under fixed-bed conditions, together with isothermal reduction/oxidation cycles, to evaluate long-term perovskite performance. Under fluidized-bed conditions, isothermal reduction/oxidation cycles were carried out as well. Results obtained under fixed-bed and fluidized-bed conditions were compared in terms of oxygen carrier reactivity and stability. The oxygen carrier showed good reactivity and stability in the range 800–1000 °C. An overall yield of 0.6 Nm3 of syngas per kg of perovskite can be reached per cycle. The decomposition of CH4 catalyzed by the reduced oxide can also occur during the reduction step. However, deposited carbon is easily re-gasified through the Boudouard reaction, without affecting the reactivity of the material. Fluidized-bed tests showed higher conversion rates compared to fixed-bed conditions and allowed better control of CH4 decomposition, with a H2:CO ratio of around 2 and CO selectivity of around 0.8. However, particle attrition was observed and might be responsible for a loss of the inventory of up to 9%w.
Effects of Drying and Rewetting Cycles on Carbon Dioxide Emissions and Soil Microbial Communities
Authors: Zhang Yun , Li Xiaohan , Liu Xinmei , Cui Yufei , Zhang Ye , Zheng Xiaoying , Zhang Weiwei , Fan Yue , Zou Junliang
Published: November 2022

Extreme rainfall and drought events attributed to climate change are anticipated to occur in the current century, resulting in frequent drying and rewetting cycles (DWCs) in soils, which will, in turn, influence soil properties and microorganisms. Sample plots of Sophora japonica, Pinus tabulaeformis, and Ginkgo biloba were selected, and undisturbed soil columns were collected. CK was the constant drying treatment; the precipitation intensities of R80, R40, and R20 were 80 mm, 40 mm, and 20 mm, respectively, and the total precipitation for the four treatments was 160 mm. Significant differences were observed in the cumulative CO2 emissions among the various DWC frequencies for the same woodland soils. A significant correlation was observed between the Birch effect and the DWC frequencies of the three woodland soils. A Pearson’s correlation analysis revealed that background nutrient contents were the key factors influencing alpha diversity. In conclusion, DWCs generally increased CO2 fluxes, cumulative CO2 emissions, and the Birch effect in addition to decreasing the alpha diversity of soil microorganisms when compared to those in the constant drying treatment.
Foil Storage of Storm-Felled Timber—Long-Term Monitoring of Norway Spruce Piles in Germany
Authors: Brischke Christian , Wippermann Kira
Published: November 2022

Windthrow and other calamities pose major challenges for forestry companies. In addition to difficult processing, solutions for the medium- and long-term storage of logs without the loss of quality are required in order to counteract the oversupply and falling prices of raw wood. The application of foil piles is a comparatively young and only regionally established procedure, which is of particular interest when wet storage is not possible. As part of a case study, a total of 32 piles of Norway spruce (Picea abies) wood were monitored under foil in the forest district of Dassel (Lower Saxony State Forests, Germany), and the conditions for wood-destroying fungi and the resulting wood quality were evaluated. A considerable depreciation in the stored wood was directly related to the atmosphere inside the pile and could be attributed to various types of damage to the foil. It became evident that quality losses can only be avoided by working quickly, being careful when sealing the piles and providing intensive support in the form of monitoring and rapid repairs.
Fermented Foods of Korea and Their Functionalities
Authors: Jung Su-Jin , Chae Soo-Wan , Shin Dong-Hwa
Published: November 2022

Fermented foods are loved and enjoyed worldwide and are part of a tradition in several regions of the world. Koreans have traditionally had a healthy diet since people in this region have followed a fermented-foods diet for at least 5000 years. Fermented-product footprints are evolving beyond boundaries and taking the lead in the world of food. Fermented foods, such as jang (fermented soybean products), kimchi (fermented vegetables), jeotgal (fermented fish), and vinegar (liquor with grain and fruit fermentation), are prominent fermented foods in the Korean culture. These four major fermented foods have been passed down through the generations and define Korean cuisine. However, scientific advancements in the fermentation process have increased productivity rates and facilitated global exports. Recently, Korean kimchi and jang have garnered significant attention due to their nutritional and health-beneficial properties. The health benefits of various Korean fermented foods have been consistently supported by both preclinical and clinical research. Korean fermented foods effectively reduce the risk of cardiovascular and chronic metabolic diseases, such as immune regulation, memory improvement, obesity, diabetes, and high blood pressure. Additionally, kimchi is known to prevent and improve multiple metabolic diseases, including irritable bowel syndrome (IBS), and improve beneficial intestinal bacteria. These functional health benefits may reflect the synergistic effect between raw materials and various physiologically active substances produced during fermentation. Thus, fermented foods all over the world not only enrich our dining table with taste, aroma, and nutrition, but also the microorganisms involved in fermentation and metabolites of various fermentations have a profound effect on human health. This article describes the production and physiological functions of Korean fermented foods, which are anticipated to play a significant role in the wellness of the world’s population in the coming decades.
Prediction Models for the Content of Calcium, Boron and Potassium in the Fruit of ‘Huangguan’ Pears Established by Using Near-Infrared Spectroscopy
Authors: Fang Jing , Jin Xiu , Wu Lin , Zhang Yuxin , Jia Bing , Ye Zhenfeng , Heng Wei , Liu Li
Published: November 2022

It has been proved that the imbalance of the proportion of elements of ‘Huangguan’ pears in the pulp and peel, especially calcium, boron and potassium, may be important factors that can seriously affect the pears’ appearance quality and economic benefits. The objective of this study was to predict the content of calcium, boron and potassium in the pulp and peel of ‘Huangguan’ pears nondestructively and conveniently by using near-infrared spectroscopy (900–1700 nm) technology. Firstly, 12 algorithms were used to preprocess the original spectral data. Then, based on the original and preprocessed spectral data, full-band prediction models were established by using Partial Least Squares Regression and Gradient Boosting Regression Tree. Finally, the characteristic wavelengths were extracted by Genetic Algorithms to establish the characteristic wavelength prediction models. According to the prediction results, the value of the determination coefficient of the prediction sets of the best prediction models for the three elements all reached ideal levels, and the values of their Relative analysis error also showed high levels. Therefore, the micro near-infrared spectrometer based on machine learning can predict the content of calcium, boron and potassium in the pulp and peel of ‘Huangguan’ pears accurately and quickly. The results also provide an important scientific theoretical basis for further research on the degradation of the quality of ‘Huangguan’ pears caused by a lack of nutrients.
Analysis of the Small Auxin-Up RNA (SAUR) Genes Regulating Root Growth Angle (RGA) in Apple
Authors: Zhou Yufei , Lan Qingqing , Yu Wenhan , Zhou Yuwen , Ma Shuya , Bao Zeyang , Li Xu , Zheng Caixia
Published: November 2022

Small auxin upregulated RNAs (SAURs) are primary auxin response genes; the function of regulating root growth angle (RGA) is unclear in the apple rootstock. We firstly identified 96 MdSAUR genes families from new apple genome GDDH13 using the resequence database of ‘Baleng Crab (BC)’ and ‘M9’. A total of 25 MdSAUR genes, regulating the formation of RGA, were screened for the expression profiles in stems and roots and the allelic variants of quantitative trait loci (QTL). Finally, through the joint analysis of network and protein–protein interaction, MdSAUR2, MdSAUR29, MdSAUR60, MdSAUR62, MdSAUR69, MdSAUR71, and MdSAUR84 were screened as the main candidate genes for regulating RGA. This study provides a new insight for further revealing the regulatory mechanism of RGA in apple dwarf rootstocks.
Mechanism of Stone (Hardened Endocarp) Formation in Fruits: An Attempt toward Pitless Fruits, and Its Advantages and Disadvantages
Authors: Khan Muhammad Khalil Ullah , Muhammad Noor , Jia Zhuolong , Peng Jianying , Liu Mengjun
Published: November 2022

Stone (hardened endocarp) has a very important role in the continuity of plant life. Nature has gifted plants with various seed protection and dispersal strategies. Stone-fruit-bearing species have evolved a unique adaptation in which the seed is encased in an extremely hard wood-like shell called the stone. The lignification of the fruit endocarp layer produces the stone, a feature that separates drupes from other plants. Stone cells emerge from parenchyma cells after programmed cell death and the deposition of cellulose and lignin in the secondary cell wall. Generally, the deposition of lignin in primary cell walls is followed by secondary thickening of cell walls to form stone cells. This review article describes the molecular mechanisms and factors that influence the production of stone in the fruit. This is the first review article that describes the molecular mechanisms regulating stone (harden endocarp) formation in fruits. This article will help breeders understand the molecular and genetic basis for the stone formation in fruit, and this could lead to new and innovative directions to breed stoneless fruit cultivars in the future.
Characterization of the Evolutionary Pressure on Anisodus tanguticus Maxim. with Complete Chloroplast Genome Sequence
Authors: Zhou Dangwei , Mehmood Furrukh , Lin Pengcheng , Cheng Tingfeng , Wang Huan , Shi Shenbo , Zhang Jinkui , Meng Jing , Zheng Kun , Poczai Péter
Published: November 2022

Anisodus tanguticus Maxim. (Solanaceae), a traditional endangered Tibetan herb, is endemic to the Qinghai–Tibet Plateau. Here, we report the de novo assembled chloroplast (cp) genome sequences of A. tanguticus (155,765 bp). The cp contains a pair of inverted repeated (IRa and IRb) regions of 25,881 bp that are separated by a large single copy (LSC) region (86,516 bp) and a small single copy SSC (17,487 bp) region. A total of 132 functional genes were annotated in the cp genome, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Moreover, 199 simple sequence repeats (SSR) and 65 repeat structures were detected. Comparative plastome analyses revealed a conserved gene order and high similarity of protein-coding sequences. The A. tanguticus cp genome exhibits contraction and expansion, which differs from Przewalskia tangutica and other related Solanaceae species. We identified 30 highly polymorphic regions, mostly belonging to intergenic spacer regions (IGS), which may be suitable for the development of robust and cost-effective markers for inferring the phylogeny of the genus Anisodus and family Solanaceae. Analysis of the Ka/Ks ratios of the Hyoscyameae tribe revealed significant positive selection exerted on the cemA, rpoC2, and clpP genes, which suggests that protein metabolism may be an important strategy for A. tanguticus and other species in Hyoscyameae in adapting to the adverse environment on the Qinghai–Tibetan Plateau. Phylogenetic analysis revealed that A. tanguticus clustered closer with Hyoscyamus niger than P. tangutica. Our results provide reliable genetic information for future exploration of the taxonomy and phylogenetic evolution of the Hyoscyameae tribe and related species.
Effect of Sacubitril-Valsartan on Quality of Life, Functional and Exercise Capacity in Heart Failure with Preserved Ejection Fraction (HFpEF): A Systematic Review of Randomized Clinical Trials
Authors: Vasavada Advait , Sadhu Akhil , Valencia Carla , Fatima Hameeda , Nwankwo Ijeoma , Anam Mahvish , Maharjan Shrinkhala , Amjad Zainab , Abaza Abdelrahman , Khan Safeera
Published: November 2022

Background: Sacubitril/Valsartan use in heart failure has shown promising results in early trials. However, the effects on the overall functional capacity, exercise capacity, and quality of life are unknown. Aims: We aimed to understand the results of studies that attempted to measure these outcomes that affect the mobility and day-to-day life of these patients. Methods: MEDLINE, PubMed, PubMed Central (PMC), Google Scholar, ClinicalTrials.gov, and ISRCTN were explored to look for clinical trials relevant to the literature. Results: A total of three high-quality randomized controlled trials were discovered that evaluated the effect of sacubitril-valsartan on functional capacity, exercise capacity, or quality of life. All of them were industry-funded and revealed no statistical difference in the mentioned outcomes. No study measured peak oxygen uptake or ventilation/carbon dioxide ratio slope. Conclusion: Sacubitril-valsartan had minimal to no impact on functional capacity, exercise capacity, or quality of life. However, future prospective studies with more sensitive outcome measures should be conducted to validate the findings.
Operational and Financial Impacts of Digital Health Technology: A Study on Canadian Healthcare System during the COVID-19 Pandemic
Authors: Jin Zixin (Jessie) , Wang Zongjie
Published: November 2022

During COVID-19, hospital capacity was significantly reduced to limit the spread of the pandemic. The limitations affected the efficiency of service delivery. We examined the effects of pandemic-related challenges on patient experience and hypothesize that digital health implementation increased patient satisfaction. We surveyed nationally aggregated data in hospital occupancy, hospital funding and patient experience, and plotted their correlation. We found digital health to contribute to patient experience and service-delivery effectiveness. We evaluate the benefits of digital health in context of hospital service delivery. Post-COVID-19, we recommend a continued implementation of digital health and offer suggestions to further improve its efficiency and cost-effectiveness.
A Cross-Sectional Analysis of Paternal Intimacy Problems, Stress Levels, and Satisfaction from Families with Children Born with Mucoviscidosis
Authors: Popa Zoran Laurentiu , Margan Madalin-Marius , Bernad Elena , Stelea Lavinia , Craina Marius , Ciuca Ioana Mihaela , Bina Anca Mihaela
Published: November 2022

There is an increasing interest in father–child interactions and their effects. Due to the rising number of working mothers, marital interruptions, divorces, and child custody arrangements, paternal duties and the relevance of fathering continue to be re-evaluated. As there are rising expectations for men to undertake more childcare and household responsibilities, it was hypothesized that the presence of a disabled or chronically ill child would have a significant impact on the couple’s future family situation, marital conduct due to paternal dissatisfaction, and increased stress levels. Therefore, the purpose of this study was to examine paternal intimacy problems, stress levels, and couple satisfaction inside families that have children with cystic fibrosis. The study followed a cross-sectional design with five questionnaires that were answered by a total of 107 fathers of children with cystic fibrosis from the “cases” group as the reference group, and 124 fathers of healthy children from the “control” group. The statistically significant findings of the current study show that men who were taking care of their child with mucoviscidosis engaged less frequently in sexual activity. A significantly higher number of these respondents were smokers. A higher proportion of them reported marital distress (OR = 2.54) and inhibited sexual desire (OR = 2.02), all in association with a higher number of men taking psychiatric medication (7.5% vs. 1.6%). More than 40% of all respondents declared high levels of general stress and parenting distress, while the most frequently used coping mechanism for stress was avoidance-oriented (45.8% vs. 25.8%). Other important findings were the high levels of dissatisfaction and lower levels of marital quality on the SII scale, equivalent to the intimacy problems on the MIQ scale. It is likely that paternal stress is higher when parenting children with cystic fibrosis, and the lack of intervention in this vulnerable group seem to be associated with intimacy problems, couple dissatisfaction, and maladaptive coping mechanisms. It is recommended that these concerns should not only be raised for the mothers of children with mucoviscidosis, but also for the child’s father or the male caretaker partner since they might experience the same problems as the opposite gender.
Moral Distress of Intensive Care Nurses: A Phenomenological Qualitative Study Two Years after the First Wave of the COVID-19 Pandemic
Authors: Rosa Debora , Bonetti Loris , Villa Giulia , Allieri Sara , Baldrighi Riccardo , Elisei Rolando Francesco , Ripa Paola , Giannetta Noemi , Amigoni Carla , Manara Duilio Fiorenzo
Published: November 2022

Background: The COVID-19 pandemic has imposed great pressure on healthcare facilities, exposing healthcare professionals to various challenges that may result in the onset of moral distress, a condition of psychological distress caused by the inability to act as it would be most morally appropriate. The purpose of this research was to investigate the experience lived by nurses who worked in an intensive care unit during the COVID-19 pandemic. Methods: This is a phenomenological study using interpretative phenomenological analysis. Sixteen nurses who worked in the COVID-19 Intensive Care Unit of Northern Italian Hospitals from January to April 2022 were selected through purposive sampling. Data on experiences, thoughts, and symptoms were collected through semi-structured interviews with in-person and remote modalities. Results: Five themes and fourteen subthemes emerged from the study. The themes are: (1) pride, isolation, and fear; (2) teamwork and organisation; (3) moral/ethical aspect; (4) true heroes; and (5) dignity. Conclusions: This study highlights the impact of the COVID-19 pandemic on intensive care unit nurses. It has emerged that the risk of moral distress is high among healthcare workers in the front line of the fight against the virus. This condition should be avoided and managed through early psychological interventions, sharing of experiences, and a good organization that supports decision-making and professional well-being.
Relationship between Asymmetries and Functional Autonomy in Older Chilean Adults
Authors: Huerta Ojeda Álvaro , Toro-Zepeda Vanieska , Jofré-Saldía Emilio , Bravo Maximiliano , Parra Carol , Campos-Armijo Gaspar , Jorquera-Aguilera Carlos , Albornoz Hernández Makarena , Yeomans-Cabrera María-Mercedes , Galdames Maliqueo Sergio
Published: November 2022

The objectives of this study were: (a) to determine asymmetries, both lower limb (LL) and upper limb (UL), in Chilean older adults, and (b) to relate asymmetries to FA in both LL and UL. Forty-one older adults voluntarily participated in this study (mean ± standard deviation [SD]: age 72.0 ± 8.0 years, LL asymmetries 13.78 ± 14.87%, UL asymmetries 10.70 ± 8.85%, FA 40.35 ± 16.26 points). The variables were: (1) asymmetries of LL and UL, assessed through a force platform and handgrip, respectively; (2) FA, assessed through the Latin American Group for Maturity (GDLAM) and the GDLAM index of autonomy (GI) protocol. The relationship between the variables was performed through Spearman’s correlation. The analysis showed that 39% of the participants presented asymmetries above 15% in the LL. Likewise, this 39% of older adults presented a lower FA than their peers with asymmetries below 15% in the LL (≤15%: 35.64 ± 12.26 points vs. >15%: 47.69 ± 19.23 points, p = 0.003). The analysis showed a small correlation between LL and GI asymmetries (r = 0.27, p = 0.07) and a small but negative correlation between UL and GI (r = −0.21). The mean values of asymmetries of both LL and UL are within ‘normal’ parameters. However, several older adults were identified as being at risk. In parallel, older adults who presented a higher level of asymmetries in LL showed a lower level of FA.
Lifestyle of Families with Children Aged 4–8 Years before and during Lockdown Due to COVID-19 Pandemic in Poland
Authors: Szczepańska Elżbieta , Janota Barbara
Published: November 2022

The aim of the study was to evaluate the lifestyle changes of families with children aged 4–8 years during lockdown compared to the time before the COVID-19 pandemic. The study was conducted among 1098 parents during the first lockdown in Poland. An originally developed questionnaire was used as the research tool. The Wilcoxon test was used to determine the frequency of differences in the lifestyle of parents and children before the pandemic and during lockdown. Differences were found in the frequency of healthy habits in the periods under investigation, both in the lifestyle of parents and children. A moderately healthy lifestyle was predominant among families with children aged 4–8 years during lockdown. The families’ lifestyle significantly changed in relation to the time before the pandemic. There is a need for lifestyle education for families with children to prevent the development of bad habits during and after the pandemic and isolation time.
Social Overview of Smartphone Use by Teenagers
Authors: Ricoy María-Carmen , Martínez-Carrera Sara , Martínez-Carrera Isabel
Published: November 2022

Information and Communication Technologies have led to a new way of life and, in particular, of socialization. The objective of this study is to analyse the image social media disseminate of news taken from digital newspapers, based on the opportunities and drawbacks attributed to smartphone use by teenagers. An essentially qualitative methodology was used, on a sample of 1704 news items published in digital newspapers. The results and conclusions show that smartphone use by teenagers improves development of their digital competence, presents new academic opportunities (through gamification or mobile learning) and provides them with digital tools for school and leisure. The widespread drawbacks reflect the effects of the device on the deterioration of health (dependence, stress, psychosocial problems) and emotions, thereby succinctly affecting academic performance. A noticeable increase of positive news about smartphones was published in the major newspapers in December, while that on its negative effects, in September.
Three Pairs of Novel Enantiomeric 8-O-4′ Type Neolignans from Saussurea medusa and Their Anti-inflammatory Effects In Vitro
Authors: Cao Jing-Ya , Dong Qi , Wang Zhi-Yao , Mei Li-Juan , Tao Yan-Duo , Yu Rui-Tao
Published: November 2022

Three pairs of novel enantiomeric 8-O-4′ type neolignans (1a/1b–3a/3b), together with seven known analogues (4–10), were isolated from the whole plants of Saussurea medusa. Their structures were elucidated by extensive spectroscopic data analysis and electric circular dichroism (ECD) calculations after chiral separations. All compounds were obtained from S. medusa for the first time, and compounds 1–3 and 5–10 had never been obtained from the genus Saussurea previously. The anti-inflammatory activities of the compounds were evaluated by determining their inhibitory activities on the production of NO and inducible nitric oxide synthase (iNOS) expression in LPS-stimulated RAW 264.7 cells. Compounds (+)-1a, (−)-1b and 5–7 inhibited NO production and had IC50 values ranging from 14.3 ± 1.6 to 41.4 ± 3.1 μM. Compound 7 induced a dose-dependent reduction in the expression of iNOS in LPS-treated RAW 264.7 cells. Molecular docking experiments showed that all active compounds exhibited excellent docking scores (<−7.0 kcal/mol) with iNOS. Therefore, compounds (+)-1a, (−)-1b and 5–7 isolated from the whole plants of S. medusa may have therapeutic potential in inflammatory diseases.
A R2R3 MYB Transcription Factor, TaMYB391, Is Positively Involved in Wheat Resistance to Puccinia striiformis f. sp. tritici
Authors: Hawku Mehari Desta , He Fuxin , Bai Xingxuan , Islam Md Ashraful , Huang Xueling , Kang Zhensheng , Guo Jun
Published: November 2022

A biotrophic fungus, Puccinia striiformis f.sp. tritici (Pst), which causes stripe rust disease in wheat is the most yield-limiting factor in wheat production. Plants have complex defense mechanisms against invading pathogens. Hypersensitive response (HR), a kind of programmed cell death (PCD) at the infection site, is among these defense mechanisms. Transcription factors (TFs) play a crucial role in plant defense response against invading pathogens. Myeloblastosis (MYB) TFs are among the largest TFs families that are involved in response to both biotic and abiotic stresses. However, little is known about the mechanisms of MYB TFs during the interaction between wheat and the stripe rust fungus. Here, we identified an R2R3 MYB TF from wheat, designated as TaMYB391, and characterized its functional role during wheat–Pst interaction. Our data indicated that TaMYB391 is induced by Pst infection and exogenous application of salicylic acid (SA) and abscisic acid (ABA). TaMYB391 is localized in the nucleus of both wheat and Nicotiana benthamiana. Transient overexpression of TaMYB391 in N. benthamiana triggered HR-related PCD accompanied by increased electrolyte leakage, high accumulation of reactive oxygen species (ROS), and transcriptional accumulation of SA defense-related genes and HR-specific marker genes. Overexpression of TaMYB391 in wheat significantly enhanced wheat resistance to stripe rust fungus through the induction of pathogenesis-related (PR) genes, ROS accumulation and hypersensitive cell death. On the other hand, RNAi-mediated silencing of TaMYB391 decreased the resistance of wheat to Pst accompanied by enhanced growth of the pathogen. Together our findings demonstrate that TaMYB391 acts as a positive regulator of HR-associated cell death and positively contributes to the resistance of wheat to the stripe rust fungus by regulating certain PR genes, possibly through SA signaling pathways.
Tumor Treating Fields (TTFields) Concomitant with Immune Checkpoint Inhibitors Are Therapeutically Effective in Non-Small Cell Lung Cancer (NSCLC) In Vivo Model
Authors: Barsheshet Yiftah , Voloshin Tali , Brant Boris , Cohen Gadi , Koren Lilach , Blatt Roni , Cahal Shay , Haj Khalil Tharwat , Zemer Tov Efrat , Paz Rom , Klein-Goldberg Anat , Tempel-Brami Catherine , Jacobovitch Sara , Volodin Alexandra , Kan Tal , Koltun Bella , David Cfir , Haber Adi , Giladi Moshe , Weinberg Uri , Palti Yoram
Published: November 2022

Tumor Treating Fields (TTFields) are electric fields that exert physical forces to disrupt cellular processes critical for cancer cell viability and tumor progression. TTFields induce anti-mitotic effects through the disruption of the mitotic spindle and abnormal chromosome segregation, which trigger several forms of cell death, including immunogenic cell death (ICD). The efficacy of TTFields concomitant with anti-programmed death-1 (anti-PD-1) treatment was previously shown in vivo and is currently under clinical investigation. Here, the potential of TTFields concomitant with anti- PD-1/anti-cytotoxic T-lymphocyte-associated protein 4 (anti-CTLA-4) or anti-programmed death-ligand 1 (anti-PD-L1) immune checkpoint inhibitors (ICI) to improve therapeutic efficacy was examined in lung tumor-bearing mice. Increased circulating levels of high mobility group box 1 protein (HMGB1) and elevated intratumoral levels of phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α) were found in the TTFields-treated mice, indicative of ICD induction. The concomitant application of TTFields and ICI led to a significant decrease in tumor volume as compared to all other groups. In addition, significant increases in the number of tumor-infiltrating immune cells, specifically cytotoxic T-cells, were observed in the TTFields plus anti-PD-1/anti-CTLA-4 or anti-PD-L1 groups. Correspondingly, cytotoxic T-cells isolated from these tumors showed higher levels of IFN-γ production. Collectively, these results suggest that TTFields have an immunoactivating role that may be leveraged for concomitant treatment with ICI to achieve better tumor control by enhancing antitumor immunity.
Sources, Selection, and Microenvironmental Preconditioning of Cells for Urethral Tissue Engineering
Authors: Xuan Zongzhe , Zachar Vladimir , Pennisi Cristian Pablo
Published: November 2022

Urethral stricture is a common urinary tract disorder in men that can be caused by iatrogenic causes, trauma, inflammation, or infection and often requires reconstructive surgery. The current therapeutic approach for complex urethral strictures usually involves reconstruction with autologous tissue from the oral mucosa. With the goal of overcoming the lack of sufficient autologous tissue and donor site morbidity, research over the past two decades has focused on cell-based tissue-engineered substitutes. While the main focus has been on autologous cells from the penile tissue, bladder, and oral cavity, stem cells from sources such as adipose tissue and urine are competing candidates for future urethral regeneration due to their ease of collection, high proliferative capacity, maturation potential, and paracrine function. This review addresses the sources, advantages, and limitations of cells for tissue engineering in the urethra and discusses recent approaches to improve cell survival, growth, and differentiation by mimicking the mechanical and biophysical properties of the extracellular environment.
Poly(ADP-ribose) in Condensates: The PARtnership of Phase Separation and Site-Specific Interactions
Authors: Alemasova Elizaveta E. , Lavrik Olga I.
Published: November 2022

Biomolecular condensates are nonmembrane cellular compartments whose formation in many cases involves phase separation (PS). Despite much research interest in this mechanism of macromolecular self-organization, the concept of PS as applied to a live cell faces certain challenges. In this review, we discuss a basic model of PS and the role of site-specific interactions and percolation in cellular PS-related events. Using a multivalent poly(ADP-ribose) molecule as an example, which has high PS-driving potential due to its structural features, we consider how site-specific interactions and network formation are involved in the formation of phase-separated cellular condensates.
Tobramycin Nanoantibiotics and Their Advantages: A Minireview
Authors: Rosalia Mariella , Chiesa Enrica , Tottoli Erika Maria , Dorati Rossella , Genta Ida , Conti Bice , Pisani Silvia
Published: November 2022

Nowadays, antimicrobial resistance (AMR) represents a challenge for antibiotic therapy, mostly involving Gram-negative bacteria. Among the strategies activated to overcome AMR, the repurposing of already available antimicrobial molecules by encapsulating them in drug delivery systems, such as nanoparticles (NPs) and also engineered NPs, seems to be promising. Tobramycin is a powerful and effective aminoglycoside, approved for complicated infections and reinfections and indicated mainly against Gram-negative bacteria, such as Pseudomonas aeruginosa, Escherichia coli, Proteus, Klebsiella, Enterobacter, Serratia, Providencia, and Citrobacter species. However, the drug presents several side effects, mostly due to dose frequency, and for this reason, it is a good candidate for nanomedicine formulation. This review paper is focused on what has been conducted in the last 20 years for the development of Tobramycin nanosized delivery systems (nanoantibiotics), with critical discussion and comparison. Tobramycin was selected as the antimicrobial drug because it is a wide-spectrum antibiotic that is effective against both Gram-positive and Gram-negative aerobic bacteria, and it is characterized by a fast bactericidal effect, even against multidrug-resistant microorganisms (MDR).