bluuebunny/crossref_metadata_2025 huggingface dataset:
Photoperiodic Regulation of Tuber Enlargement in Water Yam
Authors: Hamaoka Norimitsu , Nabeshima Misato , Moriyama Takahito , Kozawa Yudai , Ishibashi Yushi
Published: November 2022

In tuberous crops, tuber enlargement is one of the most important target traits for yield formation. It has long been known that tuber growth in yams is enhanced by short-day (SD) conditions, but the mechanism of tuber enlargement remains unknown. Here, we analyzed the photoperiodic regulation of tuber enlargement in water yam (Dioscorea alata L.). The photoperiod experiments in seedlings showed that tuber enlargement is initiated under SD conditions (≤10 h daylength) within 20 days of treatment. DaFT2, a FLOWERING LOCUS T (FT)-like gene, was upregulated in SD and downregulated in long-day (LD) conditions in tubers, suggesting that DaFT2 promotes tuber enlargement. DaFT1, the other FT-like gene, was significantly upregulated only in the leaves under LD, and its expression pattern was opposite to that of DaFT2 in the tubers. A night-break experiment showed that tuber growth was inhibited by red light in the dark period. These results suggest that the tuber enlargement of water yam is completely dependent on the photoperiod and that it involves an FT gene-mediated mechanism in response to the SD condition by red light sensing.
Antagonistic Interactions between Dicamba and Glyphosate on Barnyardgrass (Echinochloa crus-galli) and Horseweed (Erigeron canadensis) Control
Authors: Polli Estefania G. , Guimaraes Leandro H. S. , de Sanctis Jose H. S. , Kruger Greg
Published: November 2022

Dicamba plus glyphosate (DpG) tank mixture has been widely adopted for broad-spectrum weed control. However, recent studies indicated antagonistic interactions between these herbicides. Furthermore, little is known about the effect of non-ammonium sulfate water conditioner (non-AMS WC) adjuvant on the activity of DpG tank mixture. Thus, the present study was elaborated to evaluate (1) the interaction of DpG tank mixtures combinations on barnyardgrass, and glyphosate-susceptible (GS) and -resistant (GR) horseweed control, and (2) the effect of non-ammonium sulfate water conditioner (non-AMS WC) on the interaction of those two herbicides on the aforementioned weed species. Greenhouse experiments were conducted in 2020 at the Pesticide Application Laboratory in North Platte, NE. Herbicide treatments were arranged in a two-level factorial design of six dicamba rates by six glyphosate rates for Study 1, and in a three-level factorial design of two adjuvant treatments (presence or not of non-AMS WC) by four dicamba rates by four glyphosate rates for Study 2. Both trials were conducted as randomized complete block designs with four replications and two runs. Antagonistic interactions were observed throughout DpG treatments in GS and GR horseweed. For instance, dicamba (560 g ae ha−1) and glyphosate (1260 g ae ha−1) resulted in 72% of GR horseweed biomass reduction, compared to 81% of estimated biomass reduction. For barnyardgrass, antagonistic interactions were only observed within the reduced glyphosate rates. The addition of non-AMS WC had no effect on DpG antagonism. However, it improved the barnyardgrass control by glyphosate.
Addition of a Blend Based on Zinc Chloride and Lignans of Magnolia in the Diet of Broilers to Substitute for a Conventional Antibiotic: Effects on Intestinal Health, Meat Quality, and Performance
Authors: Galli Gabriela M. , Strapazzon João V. , Marchiori Maiara S. , Molosse Vitor L. , Deolindo Guilherme L. , Fracasso Mateus , Copetti Priscila M. , Morsch Vera M. , Baldissera Matheus D. , Mendes Ricardo E. , Boiago Marcel M. , da Silva Aleksandro S.
Published: November 2022

This study aimed to determine whether adding a blend based on zinc chloride and lignans from magnolia to the diet of broilers could replace conventional performance enhancers. For this study, 360 chickens were divided into four groups, with six repetitions per group (n = 15), as follows: CN, without promoter; GPC, control, 50 mg/kg of enramycin growth promoter; T-50, additive blend at a dose of 50 g/ton; and T-100, additive blend at a dose of 100 g/ton. Chickens fed with the additive blend at 50 g/ton showed a production efficiency index equal to that in the GPC group (p < 0.05). At 42 days, the lowest total bacterial count (TBC) was found in the T-100 group, followed by that in the GPC group (p < 0.001). For E. coli, the lowest count was observed in the T-100 group, followed by that in the CP and T-50 groups (p < 0.001). Higher villus/crypt ratios were observed in birds belonging to the T-100 and T-50 groups than in the GPC and NC groups (p < 0.001). Greater water retention was found in the T-50 group than in NC and T-100 groups (p < 0.048). The lowest water loss during cooking was also noted in the T-50 group (p < 0.033). We concluded that adding the antimicrobial blend, primarily at 50 g/ton, maintains the efficiency of the index of production and improves the intestinal health and meat quality of the birds.
Reduction in Mortality of Calves with Bovine Respiratory Disease in Detection with Influenza C and D Virus
Authors: Saipinta Duanghathai , Panyamongkol Tanittian , Chuammitri Phongsakorn , Suriyasathaporn Witaya
Published: November 2022

Both influenza C (ICV) and influenza D (IDV) viruses were recently included as bovine respiratory disease (BRD) causes, but their role in BRD has not been evaluated. Therefore, the mortality and reproductive performances of BRD calves with different isolated viruses were determined in this study. Data on 152 BRD calves with bovine viral diarrhoea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCoV), bovine parainfluenza virus 3 (BPIV-3), ICV, or IDV from nasal swab samples using real-time rt-PCR were used. The general data and respiratory signs were recorded immediately, and thereafter, the data on dead or culling calves due to BRD and reproductive performance were collected. The percentages of the BRD calves were 71.7%, 52.6%, 40.8%, 10.5%, 68.4%, and 65.8% for BVDV, BRSV, BCoV, BPIV-3, ICV, and IDV, respectively. Mucous secretion (OR = 4.27) and age ≤ 6 months (OR =14.97) had higher risks of mortality than those with serous secretion and older age. The calves with IDV had lower risks of culling than those without IDV (OR = 0.19). This study shows that most viral infections in BRD calves are a combination of viruses with BVDV, ICV, and IDV. In addition, IDV might have a role in reducing the severity of BRD calves.
The Application of 3D Imaging as an Appropriate Method of Wildlife Craniometry: Evaluation of Accuracy and Measurement Efficiency
Authors: Košinová Klára , Turek Jiří , Cukor Jan , Linda Rostislav , Häckel Martin , Hart Vlastimil
Published: November 2022

The suitability of CT and 3D scanners for craniometric proposes was tested using digital calipers when determining linear measurements, and a measuring cylinder was used for the accuracy of 3D printing of deer antlers obtained by the CT and 3D scanners. The resolution of digitized objects from a 3D scanner ranged from 0.008 mm to 0.122 mm. For mandibular dimensions, a positive deviation (p < 0.01) from the primary control measurement was recorded. The average antler volume measured with the cylinder was 60.47 cm3 at the first measurement, in the case of the CT scanner 61.62 cm3 and for the 3D scanner 64.76 cm3—both technologies exhibit a positive deviation from the primary measurement. Precise sensing and measurements can be used to evaluate the quality and evolution of wildlife populations, create digital museum collections, or to examine in detail certain traits such as antler and horn development or dentition.
Relationship between Reticulorumen Parameters Measured in Real Time and Methane Emission and Heat Stress Risk in Dairy Cows
Authors: Antanaitis Ramūnas , Anskienė Lina , Rapaliutė Eglė , Bilskis Ronaldas , Džermeikaitė Karina , Bačėninaitė Dovilė , Juškienė Violeta , Juška Remigijus , Meškinytė Edita
Published: November 2022

The objective of this study was to investigate a connection between CH4 emissions and reticulorumen pH and temperature. During the experiment, we registered the following parameters: reticulorumen pH (pH), reticulorumen temperature (RR temp.), reticulorumen temperature without drinking cycles, ambient temperature, ambient relative humidity, cow activity, heat index, temperature–humidity index (THI), and methane emissions (CH4). The experimental animals were divided into two groups based on the reticulorumen pH: 1. pH < 6.22 and 2. pH 6.22–6.42. We found that cows assigned to the second pH class had higher (46.18%) average values for methane emissions (p < 0.01). For the other indicators, higher average values were detected in cows of the first pH class, RR temperature (2.80%), relative humidity (20.96%), temperature–humidity index (2.47%) (p < 0.01), and temperature (3.93%) (p < 0.05), which were higher compared to cows of the second pH class. Reticulorumen pH was highly negatively correlated with THI and temperature (r = −0.667 to 0.717, p < 0.001) and somewhat negatively with heat index, relative humidity, and RR temperature (r = −0.536, p < 0.001; r = −0.471 to 0.456, p < 0.01). Cows with a higher risk of heat stress had a higher risk of lower reticulorumen pH.
Analysis of the Horizontal Distribution of Sampling Points for Gas Concentrations Monitoring in an Open-Sided Dairy Barn
Authors: D’Urso Provvidenza Rita , Arcidiacono Claudia , Cascone Giovanni
Published: November 2022

In the livestock sector, the monitoring of gas concentrations plays a relevant role in the implementation of mitigation strategies, as well as the improvement in the indoor air quality for both workers and animals in barns. In the present study, measurements of gas concentrations (NH3, CH4, and CO2) at different locations were carried out in an open dairy barn using a commercial photo-acoustic spectroscopy system. Measurement variability for different datasets was computed to contribute to the evaluation of the sampling strategy in the measurement campaign. The results showed that the position of sampling locations (SLs) significantly influenced (p < 0.001) the variability in the gas distribution. Specifically, the SLs located on the perimeter of the building had the highest variability. The number of SLs utilised for determining the mean value of gas concentration in the barn had a significant influence on NH3 (p < 0.001), CO2 (p < 0.001), and CH4 (p < 0.001) in both the central zone and the perimeter one. At least three SLs were necessary to obtain a mean value of gas concentration that reduced the variability to under the 10% in the central zone and 16% in the perimeter one. Moreover, the NH3 concentrations obtained as the mean value of the gas measurements at two SLs with a 10 m distance had a lower variability compared to those recorded at two SLs located at 5 m.
Vision Transformer in Industrial Visual Inspection
Authors: Hütten Nils , Meyes Richard , Meisen Tobias
Published: November 2022

Artificial intelligence as an approach to visual inspection in industrial applications has been considered for decades. Recent successes, driven by advances in deep learning, present a potential paradigm shift and have the potential to facilitate an automated visual inspection, even under complex environmental conditions. Thereby, convolutional neural networks (CNN) have been the de facto standard in deep-learning-based computer vision (CV) for the last 10 years. Recently, attention-based vision transformer architectures emerged and surpassed the performance of CNNs on benchmark datasets, regarding regular CV tasks, such as image classification, object detection, or segmentation. Nevertheless, despite their outstanding results, the application of vision transformers to real world visual inspection is sparse. We suspect that this is likely due to the assumption that they require enormous amounts of data to be effective. In this study, we evaluate this assumption. For this, we perform a systematic comparison of seven widely-used state-of-the-art CNN and transformer based architectures trained in three different use cases in the domain of visual damage assessment for railway freight car maintenance. We show that vision transformer models achieve at least equivalent performance to CNNs in industrial applications with sparse data available, and significantly surpass them in increasingly complex tasks.
Evaluating the Impact of Land Cover and Topography on Meteorological Parameters’ Relations and Similarities in the Alberta Oil Sands Region
Authors: Deshmukh Dhananjay , Ahmed M. Razu , Dominic John Albino , Zaghloul Mohamed S. , Gupta Anil , Achari Gopal , Hassan Quazi K.
Published: November 2022

Herein, the focus was on the identification of similarities in the weather parameters collected within 19 stations, consisting of 3 weather networks located in the Lower Athabasca River Basin operated under the Oil Sands Monitoring program. These stations were then categorised into seven distinct groups based on comparable topography and land cover. With regard to weather parameters, these were air temperature (AT), precipitation (PR), relative humidity (RH), solar radiation (SR), atmospheric/barometric pressure (BP), snowfall depth (SD), and wind speed/direction (WSD). For all seven groups, relational analysis was conducted for every station pair using Pearson’s coefficient (r) and average absolute error (AAE), except for wind direction and wind speed. Similarity analysis was also performed for each station pair across all seven groups using percentage of similarity (PS) measures. Our similarity analysis revealed that there were no similarities (i.e., PS value < 75%) for: (i) SR, PR, and WSD for all groups; (ii) AT for all groups except group G3; (iii) RH for group G7; and (iv) BP for group G1. This study could potentially be decisive in optimizing or rationalising existing weather networks. Furthermore, it could be constructive in the development of meteorological prediction models for any place and that requires input from surrounding stations.
Carbon Dots with Up-Conversion Luminescence as pH Nanosensor
Authors: Laptinskiy Kirill , Khmeleva Maria , Vervald Alexey , Burikov Sergey , Dolenko Tatiana
Published: November 2022

In this study, the up-conversion luminescence for aqueous suspensions of carbon dots with polyfunctional and carboxylated surfaces synthesized by a hydrothermal method was investigated. The obtained quadratic dependence of the luminescence intensity on the power of the exciting radiation indicates that the up-conversion luminescence of these carbon dots is caused by two-photon absorption. The optimal wavelength of the exciting radiation was determined for the studied samples. The dependences of the signal for the up-conversion luminescence of carbon dots on the pH value of the suspension were obtained. It was shown that these carbon dots can be used as the nanosensor of pH of liquid media in a wide range of pH values. The advantage of this nanosensor is that the excitation of the up-conversion luminescence of carbon dots does not entail excitation of autoluminescence of the biological medium. It expands the possibilities of using this sensor in biomedical applications.
Study on the Structural Performance of UHPC Pavement and Hinge Joint Reinforcement for Hollow Slab Girder Bridges
Authors: Ye Wenya , Li Fangyuan
Published: November 2022

To address the problem of structural performance degradation caused by hinged joints and pavement damage, we utilized actual engineering to conduct a construction study on the overall replacement of pavement and hinge joint reinforcement in ultra-high-performance concrete (UHPC) hollow slab girder bridges. A replacement and reinforcement design was developed and reconstruction was undertaken. By using UHPC and reinforcement bars, the adjacent slab girders were designed to work together under specific construction process guarantees for the characteristics of UHPC. The corresponding interface treatment and a combination of planting bars and steel mesh were necessary. According to the strain and deflection monitoring results, the overall performance of the bridge after pavement and hinge joint reinforcement was verified. The strain amplitude of the reinforcement was approximately 10 με, and that of the concrete was approximately 5 με. The deflection difference of the adjacent girder was similar, which proved that the hinge joints connect girders and transfer force effectively. All the results clearly demonstrated the positive overall effect of the UHPC replacement method. The conclusions could provide a reference for the reinforcement and reconstruction of similar projects.
A Mixed Reality-Based Platform towards Human-Cyber-Physical Systems with IoT Wearable Device for Occupational Safety and Health Training
Authors: Li Chi Ho Jimmy , Liang Vincy , Chow Yuk Ting Hester , Ng Hiu-Yin , Li Shek-Ping
Published: November 2022

Occupational safety and health (OSH) should be regarded as a crucial challenge that affects the public world widely. Work-related accidents and occupational illness contribute to considerable mortality and morbidity. As technology advances, mixed reality (MR) has gained popularity. To minimize occupational accidents occurring in the workplace and reduce human training time, an MR-based platform for OSH training combined with CPS and IoT technology is proposed in this paper. Multi-criteria decision-making (MCDM) and fuzzy-analytic hierarchy process (FAHP) were applied to evaluate and select suitable gloves. Only when the MR wearable devices are improved can a more powerful MR-based OSH training program be established. A higher immersive level of OSH training offers people a more realistic experience. They will better understand possible risks in workers’ future work, resulting in a lower occupational accident rate in the workplace.
Review of Carbon Capture and Methane Production from Carbon Dioxide
Authors: Akpasi Stephen Okiemute , Isa Yusuf Makarfi
Published: November 2022

In the last few decades, excessive greenhouse gas emissions into the atmosphere have led to significant climate change. Many approaches to reducing carbon dioxide (CO2) emissions into the atmosphere have been developed, with carbon capture and sequestration (CCS) techniques being identified as promising. Flue gas emissions that produce CO2 are currently being captured, sequestered, and used on a global scale. These techniques offer a viable way to encourage sustainability for the benefit of future generations. Finding ways to utilize flue gas emissions has received less attention from researchers in the past than CO2 capture and storage. Several problems also need to be resolved in the field of carbon capture and sequestration (CCS) technology, including those relating to cost, storage capacity, and reservoir durability. Also covered in this research is the current carbon capture and sequestration technology. This study proposes a sustainable approach combining CCS and methane production with CO2 as a feedstock, making CCS technology more practicable. By generating renewable energy, this approach provides several benefits, including the reduction of CO2 emissions and increased energy security. The conversion of CO2 into methane is a recommended practice because of the many benefits of methane, which make it potentially useful for reducing pollution and promoting sustainability.
Response of Terrestrial Net Primary Production to Quadrupled CO2 Forcing: A Comparison between the CAS-ESM2 and CMIP6 Models
Authors: Zhu Jiawen , Zeng Xiaodong , Gao Xiaofei , Zhang He
Published: November 2022

Terrestrial net primary production (NPP) is a key carbon flux that changes with rising atmospheric CO2 and CO2-induced climate change. Earth system models are commonly used to investigate these NPP changes because of their fundamentally trustworthy ability to simulate physical climate systems and terrestrial biogeochemical processes. However, many uncertainties remain in projecting NPP responses, due to their complex processes and divergent model characteristics. This study estimated NPP responses to elevated CO2 and CO2-induced climate change using the Chinese Academy of Sciences Earth System Model version 2 (CAS-ESM2), as well as 22 CMIP6 models. Based on CMIP6 pre-industrial and abruptly quadrupled CO2 experiments, the analysis focused on a comparison of the CAS-ESM2 with the multi-model ensemble (MME), and on a detection of underlying causes of their differences. We found that all of the models showed an overall enhancement in NPP, and that CAS-ESM2 projected a slightly weaker NPP enhancement than MME. This weaker NPP enhancement was the net result of much weaker NPP enhancement over the tropics, and a little stronger NPP enhancement over northern high latitudes. We further report that these differences in NPP responses between the CAS-ESM2 and MME resulted from their different behaviors in simulating NPP trends with modeling time, and are attributed to their different projections of CO2-induced climatic anomalies and different climate sensitivities. These results are favorable for understanding and further improving the performance of the CAS-ESM2 in projecting the terrestrial carbon cycle, and point towards a need for greater understanding and improvements for both physical climatic processes and the terrestrial carbon cycle.
Jurassic Palynology from “The Dinosaur Coast” of Asturias (Lastres Fm., Northwestern Spain): Palynostratigraphical and Palaeoecological Insights
Authors: Santos Artai A. , Piñuela Laura , Rodríguez-Barreiro Iván , García-Ramos José Carlos , Diez José B.
Published: November 2022

Abundant fossils of vertebrates (mainly footprints and bones of dinosaurs) and numerous invertebrates occur in the Upper Jurassic deposits of the Lastres Formation in the Asturias region, North of Spain. However, no palynological study has been published from this geological formation; therefore, much palaeoenvironmental and palaeoecological information is still unknown. In this study, a total of 62 morphospecies, belonging to 49 different morphogenera were identified, including pollen, spores, algae remains, fungi spores, dinoflagellates, foraminifera, and scolecodonts from four different locations on the Asturian coast. Spores are the dominant group of palynomorphs, both in diversity and abundance, contrasting with the minor diversity of pollen grains. The age of some key taxa indicates that the palynological assemblage cannot be older than the Kimmeridgian, suggesting a Kimmeridgian-Tithonian age. The botanical and environmental affinities of the pollen and spores indicate the presence of different plant assemblages, including plant communities from humid areas such as the margin of rivers and small freshwater ponds that were dominated by bryophytes and ferns, and a coastal plant community that would inhabit arid areas and would be dominated by gymnosperms and some pteridophytes. The SEM analyses of wood remains show the abundance of charcoalified remains suggesting that wildfires were usual in “The Dinosaur Coast” of Asturias during the Kimmeridgian.
Distribution Pattern of Mangrove Fish Communities in China
Authors: Zhao Jinfa , Li Chunhou , Wang Teng , Li Chunran , Shen Jianzhong , Liu Yong , Wu Peng
Published: November 2022

Mangroves are among the most productive marine and coastal ecosystems and play an important role in maintaining the stability and diversity of fish communities. To explore the structure of mangrove fish communities in China, we compiled previous studies, monographs, and two databases on 54 mangrove areas published in the past 30 years. Mangrove fish communities in China comprised Osteichthys (597 species) and Chondrichthyes (14 species), representing 611 species in 344 genera, 117 families, and 28 orders. Perciformes were the predominant taxon, with 350 species in 52 families, accounting for 57% of the total species richness. Reef fish accounted for 29.62%. With regard to feeding groups, there were 328 carnivorous species (53.68%), 214 omnivorous species (35.02%), 41 herbivorous species (6.71%), and 28 detritivores species (4.58%). Classified by body size, 57.61% were small-sized, 24.22% medium-sized, and 18.17% were large-sized fishes. A total of 5.23% (32 species) of these mangrove fish are currently on IUCN red lists, i.e., 2 species are critically endangered, 4 are endangered, 12 are vulnerable, and 14 are near threatened. Cluster analyses shows that Chinese mangroves fish were divided into two categories, i.e., coastal mangrove and island mangrove type. This is closely related to the distribution of reef fish. Moreover, the number of fish species showed a strong positive correlation with mangrove area, but not with latitude. The main reasons may be the subtropical and tropical geographic locations, as well as the characteristics of the South China Sea and the Taiwan Warm Current. The size and integrity of mangrove area are crucial to the local ecosystems; thus, protecting and restoring mangroves is of great significance to large-scale ecosystem-stability and local biodiversity.
One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP
Authors: Nekrasov Nikita , Kudriavtseva Anastasiia , Orlov Alexey V. , Gadjanski Ivana , Nikitin Petr I. , Bobrinetskiy Ivan , Knežević Nikola Ž.
Published: November 2022

A novel photochemical technological route for one-step functionalization of a graphene surface with an azide-modified DNA aptamer for biomarkers is developed. The methodology is demonstrated for the functionalization of a DNA aptamer for an N-terminal B-type natriuretic peptide (NT-proBNP) heart failure biomarker on the surface of a graphene channel within a system based on a liquid-gated graphene field effect transistor (GFET). The limit of detection (LOD) of the aptamer-functionalized sensor is 0.01 pg/mL with short response time (75 s) for clinically relevant concentrations of the cardiac biomarker, which could be of relevance for point-of-care (POC) applications. The novel methodology could be applicable for the development of different graphene-based biosensors for fast, stable, real-time, and highly sensitive detection of disease markers.
Identifying Effective Managerial Factors in Improving and Renovating Old Urban Tissues: A Case Study Approach
Authors: Cao Yu , Fu Fangyu , Nejati Fatemeh , Chabok Samaneh Hoseinpoorian , Edalatpanah Seyyed Ahmad
Published: November 2022

This paper attempts to identify the effective managerial factors in renovating old building tissues in a city in Iran (Langrud). The present research is practical in terms of purpose, and it is descriptive and contextual in terms of data collection. Furthermore, since this is a mixed research study from both the perspective of its nature and purpose, we conducted the study with both qualitative (interviews) and quantitative (questionnaires) methods. As the statistical population in the qualitative section consists of experts on improving worn-out tissues, we used an available sampling method and took into account the individuals’ characteristics in the sampling process. Seven managers, assistants, and engineers with more work experience than the rest of the managers and assistants were selected. During the quantitative phase, the population included all the municipality staff and the engineering system organization of Langrod city, which contains a total of 650 people. A total of 335 people were selected, and the questionnaire was distributed using Cochran’s formula. A semi-structured interview and a questionnaire were used as research tools distributed among participants. The validity and reliability of the questionnaires were determined based on existing standards. Additionally, the data were analyzed using Factor Analysis (FA), the Fuzzy Analytic Hierarchy Process (FAHP), and Structural Equations Modeling. According to the results, the most effective managerial factors and indicators in the improvement and renovation of the city’s old tissues were related to resources. Next, attention to training, commitment to environmental assessment, idea creation, planning, management, technical factors, experience, attention to legal requirements, and attention to external factors are placed.
LINC00491 Facilitates Tumor Progression of Lung Adenocarcinoma via Wnt/β-Catenin-Signaling Pathway by Regulating MTSS1 Ubiquitination
Authors: Wan Huimin , Lin Tingting , Shan Mengtian , Lu Jingjing , Guo Zhongliang
Published: November 2022

Background: Long non-coding RNAs have been reported to be involved in tumorigenesis and progression through different regulatory mechanisms. It has been reported that aberrantly expressed long non-coding RNA LINC00491 promotes malignancy in multiple tumors, while the role of LINC00491 in lung adenocarcinoma (LUAD) is little reported and the mechanism for regulating tumor progression has not been elucidated. Methods: RNA sequencing and the TCGA database were combined to screen differentially expressed lncRNAs that facilitate tumor progression. The expression level of LINC00491 was examined in LUAD clinical samples and in cell lines using RT-qPCR. In vitro experiments including colony formation assay, EdU assay, cell migration and invasion assay and wound healing assay, and in vivo experiments including xenografting subcutaneous tumors and lung metastasis models were performed to investigate the function of LINC00491 in LUAD tumor progressions. RNA pull-down, mass spectrometry, RIP assays and truncation experiments were carried out to explore the proteins binding to LINC00491 and the specific interactions between the RNA–protein complex. Results: Our results showed that LINC0491 was significantly upregulated in LUAD and positively correlated with poor survival. High LINC00491 expression promoted proliferation, migration and invasion, and resulted in a high metastatic burden in LUAD. Using pull-down assay and mass spectrometry, MTSS1 was found binding to LINC00491, and the conducted experiments verified the direct interaction between LINC00491 and MTSS1. Meanwhile, LINC00491 was found to regulate MTSS1 degradation by promoting the MTSS1 ubiquitination level and then activating the Wnt/β-catenin-signaling pathway. LINC00491/MTSS1/β-catenin may act as a complex to facilitate tumor progression. Conclusions: In summary, our results found a novel mechanism in which LINC00491 directly interacts with MTSS1 by affecting its ubiquitination modification to promote LUAD proliferation, migration and invasion, then activating the Wnt/β-catenin-signaling pathway, demonstrating its significant role in tumor progression and suggesting that the LINC00491/MTSS1/Wnt/β-catenin-signaling pathway could serve as a potential therapeutic target for lung adenocarcinoma in the future.
Short Linear Motifs in Colorectal Cancer Interactome and Tumorigenesis
Authors: Fasano Candida , Grossi Valentina , Forte Giovanna , Simone Cristiano
Published: November 2022

Colorectal tumorigenesis is driven by alterations in genes and proteins responsible for cancer initiation, progression, and invasion. This multistage process is based on a dense network of protein–protein interactions (PPIs) that become dysregulated as a result of changes in various cell signaling effectors. PPIs in signaling and regulatory networks are known to be mediated by short linear motifs (SLiMs), which are conserved contiguous regions of 3–10 amino acids within interacting protein domains. SLiMs are the minimum sequences required for modulating cellular PPI networks. Thus, several in silico approaches have been developed to predict and analyze SLiM-mediated PPIs. In this review, we focus on emerging evidence supporting a crucial role for SLiMs in driver pathways that are disrupted in colorectal cancer (CRC) tumorigenesis and related PPI network alterations. As a result, SLiMs, along with short peptides, are attracting the interest of researchers to devise small molecules amenable to be used as novel anti-CRC targeted therapies. Overall, the characterization of SLiMs mediating crucial PPIs in CRC may foster the development of more specific combined pharmacological approaches.
Binding and Detoxification of Insecticides by Potentially Probiotic Lactic Acid Bacteria Isolated from Honeybee (Apis mellifera L.) Environment—An In Vitro Study
Authors: Leska Aleksandra , Nowak Adriana , Miśkiewicz Karolina , Rosicka-Kaczmarek Justyna
Published: November 2022

Lactic acid bacteria (LAB) naturally inhabiting the digestive tract of honeybees are known for their ability to detoxify xenobiotics. The effect of chlorpyrifos, coumaphos, and imidacloprid on the growth of LAB strains was tested. All strains showed high resistance to these insecticides. Subsequently, the insecticide binding ability of LAB was investigated. Coumaphos and chlorpyrifos were bound to the greatest extent (up to approx. 64%), and imidacloprid to a much weaker extent (up to approx. 36%). The insecticides were detected in extra- and intracellular extracts of the bacterial cell wall. The ability of selected LAB to reduce the cyto- and genotoxicity of insecticides was tested on two normal (ovarian insect Sf-9 and rat intestinal IEC-6) cell lines and one cancer (human intestinal Caco-2) cell line. All strains exhibited various levels of reduction in the cyto- and genotoxicity of tested insecticides. It seems that coumaphos was detoxified most potently. The detoxification abilities depended on the insecticide, LAB strain, and cell line. The detoxification of insecticides in the organisms of honeybees may reduce the likelihood of the penetration of these toxins into honeybee products consumed by humans and may contribute to the improvement of the condition in apiaries and honeybee health.
Aging Fibroblasts Adversely Affect Extracellular Matrix Formation via the Senescent Humoral Factor Ependymin-Related Protein 1
Authors: Takaya Kento , Asou Toru , Kishi Kazuo
Published: November 2022

Skin senescence is characterized by a decrease in extracellular matrix and the accumulation of senescent fibroblasts in the dermis, and their secretion of humoral factors. Ependymin-related protein 1 (EPDR1) is involved in abnormal fibroblast metabolism and collagen deposition, however, its relation to skin aging is unclear. We investigated whether and how EPDR1 is involved in age-related dermal deterioration. When young dermal fibroblasts and senescent cells were co-cultured in a semipermeable membrane separation system, the young fibroblasts showed decreased gene expression of collagen type I α1 chain (COL1A1) and elastin, and increased expression of matrix metalloproteinase (MMP)1 and MMP3. Senescence marker expression and EPDR1 production were increased in the culture medium of senescent cells. Treatment of young fibroblasts with recombinant EPDR1, enhanced matrix-related gene expression and suppressed COL1A1 expression, whereas EPDR1 knockdown had the opposite effects. EPDR1 gene and protein expression were increased in aged skin, compared to young skin. These results suggest that senescent cells affect nearby fibroblasts, in part through EPDR1 secretion, and exert negative effects on matrix production in the dermis. These results may lead to the discovery of potential candidate targets in the development of skin anti-aging therapies.
Development and In Vitro Differentiation of Schwann Cells
Authors: Hörner Sarah Janice , Couturier Nathalie , Gueiber Daniele Caroline , Hafner Mathias , Rudolf Rüdiger
Published: November 2022

Schwann cells are glial cells of the peripheral nervous system. They exist in several subtypes and perform a variety of functions in nerves. Their derivation and culture in vitro are interesting for applications ranging from disease modeling to tissue engineering. Since primary human Schwann cells are challenging to obtain in large quantities, in vitro differentiation from other cell types presents an alternative. Here, we first review the current knowledge on the developmental signaling mechanisms that determine neural crest and Schwann cell differentiation in vivo. Next, an overview of studies on the in vitro differentiation of Schwann cells from multipotent stem cell sources is provided. The molecules frequently used in those protocols and their involvement in the relevant signaling pathways are put into context and discussed. Focusing on hiPSC- and hESC-based studies, different protocols are described and compared, regarding cell sources, differentiation methods, characterization of cells, and protocol efficiency. A brief insight into developments regarding the culture and differentiation of Schwann cells in 3D is given. In summary, this contribution provides an overview of the current resources and methods for the differentiation of Schwann cells, it supports the comparison and refinement of protocols and aids the choice of suitable methods for specific applications.
The State of Cancer Care in the United Arab Emirates in 2022
Authors: Al-Shamsi Humaid O.
Published: November 2022

Cancer is the third-leading cause of death in the United Arab Emirates (UAE); cancer care in the UAE has evolved dramatically over the last 40 years, from a single center in Al Ain in 1981 to more than 30 cancer centers and clinics across the UAE, with at least four comprehensive cancer centers in the UAE nowadays. Despite the significant progress in medical care, cancer quality control across the UAE is still lacking, with significant variations in cancer care across the cancer centers. Access to clinical trials is still hampered by a lack of expertise and research infrastructure and a small population, which renders patient accrual for trials a major challenge. Education and training are other areas for improvement that require immediate attention, and, in this review, we try to address these critical aspects for stakeholders to consider better cancer care in the UAE. Early cancer detection and screening are still evolving in the UAE, and a national screening program is lacking. There is also a need to address barriers to screening and to consider less invasive screening methods such as approved blood-based screening, which is likely to be more acceptable to the UAE population. In this review, we also address new topics that have not been addressed previously, including oncology medical tourism, psycho-oncology, onco-fertility, precision oncology, survivorship, oncology nursing, cancer support programs, and the oncology sector’s response to the COVID-19 pandemic, all in the context of the UAE cancer landscape. Finally, we provide recommendations for policymakers, regulators, payers, patient advocacy groups, and the UAE oncology community regarding the delivery and future planning of high-quality cancer care. These recommendations are aligned with the UAE government’s vision to reduce cancer mortality and provide high-quality healthcare for its citizens and residents.
Fabrication and Characterization of Oxygen-Carbon-Contained CrMnFeCoNi Coatings Electrodeposited in DMF-CH3CN Solution with and without Supporting Electrolyte LiClO4
Authors: Xiao Tian , Wang Rongguang , Tanabe Eishi , Satoh Yuhki , Bazzaoui Mohammed , Ling Yunhan , Lu Zhaoxia
Published: November 2022

Oxygen-carbon-contained CrMnFeCoNi coatings were electrodeposited in an organic solvent of DMF-CH3CN (N,N-dimethylformamide-acetonitrile) containing chlorides of chromium, manganese, iron, cobalt, and nickel, with or without supporting electrolyte lithium perchlorate (LiClO4). The coatings’ composition, structure, hardness, and electrocatalytic properties were examined. The presence of lithium in coatings obtained with LiClO4 showed it unsuitable as a supporting electrolyte in this fabrication process. Without LiClO4, oxygen-carbon-contained CrMnFeCoNi coatings were obtained at constant potentials at −2.0, −2.5, and −3.0 V (vs. SSE) by enhancing the concentrations of the target metal ions in the solution. These coatings were mainly amorphous with nanocrystals, which were comprised of >50 atom% oxygen atoms, >7 atom% carbon atoms, and near equiatomic Cr, Mn, Fe, Co, and Ni, showing a high level of hardness and advanced electrocatalytic activity in the oxygen evolution reaction.
Prediction of Target Erosion for Planar Magnetron Sputtering Systems
Authors: Kolesnikov Alexander , Kryukov Yury , Gafurov Marat , Bodnarchuk Viktor
Published: November 2022

Based on the analysis of the correlation between plasma glow and the magnetic field over a magnetron target, a model for predicting the shape of the target erosion of MSS is proposed. The magnetic field distribution is obtained upon direct measurement or calculation using the ELCUT software, which allows one to calculate the magnetic field based on the magnetron model. A special software has been developed for the calculation of the depletion profile of a target in a planar MSS. It allows one to predict the target erosion during the design phase of MSS, thus reducing the efforts required for designing a multi-magnet system of magnetron. The software has to be tested by comparing the calculated profile of the target erosion with the real one for different types of MSS.
A Distribution-Preserving Under-Sampling Method for Imbalance Defect Recognition in Castings
Authors: Yu Han , Li Xinyue , Li Xingjie , Hou Chunyu , Liu Shangyu , Xie Huasheng
Published: November 2022

Data imbalance is a crucial factor that limits the performance of automatic defect recognition systems in castings. The bias and deterioration of the model are generated by massive normal samples and minor defect samples. Traditional re-sampling methods randomly change the data distribution and ignore the significant intra-class difference among all normal samples. Therefore, this paper proposes a distribution-preserving under-sampling method for imbalance defect-recognition in castings. In detail, our method divides all normal samples into several sub-groups by cluster analysis and reassembles them into some balance datasets, which makes the normal samples in all balance datasets have an identical distribution with the original imbalance dataset. Finally, experiments on our dataset with 3260 images indicate that the proposed method achieves a 0.816 AUC (area under curve) score, which demonstrates significant advantages compared to cost-sensitive learning and re-sampling methods.
Highly Differentiated Follicular Carcinoma of Ovarian Origin: A Systematic Review of the Literature
Authors: Giovannopoulou Eirini , Saliaris Konstantinos , Kavoura Evangelia , Pavlakis Kitty , Lathouras Konstantinos
Published: November 2022

(1) Background: Highly differentiated follicular carcinoma of ovarian origin (HDFCO) is an extremely uncommon neoplasm, associated with struma ovarii. There are scarce cases reported in the literature and, subsequently, no reliable conclusions on its pathophysiology, treatment, and prognosis can be drawn. The goal of this study is to enrich the literature on the topic by adding our own experience with a case, and simultaneously accumulate all cases published up to date. (2) Methods: The present review was performed in accordance with the guidelines for systematic reviews and meta-analyses (PRISMA). PubMed (1966–2022), Scopus (2004–2022), and Clinicaltrials.gov databases were screened for relevant articles published up to July 2022. (3) Results: Twenty patients with HDFCO were identified. The included patients were aged 47.15 years (range 24–74). The predominant origin was ovarian (60%) and extraperitoneal spread was confirmed in 15% of the cases. Surgical treatment varied from conservative to radical (35.3% vs. 41.2%, respectively) and the administration of supplementary therapy and thyroidectomy was not universal. Combined thyroidectomy/radioactive iodine therapy was applied in just 62.5% of the reported cases. There was one patient who demonstrated disease recurrence and lives with the disease. No disease related morbidity was reported. (4) Conclusions: HDFCO represents a low-grade malignant tumor, whose rarity does not allow for reliable conclusions. Standard treatment including complete surgical excision and supplementary treatment seems to offer a favorable prognosis in selected cases.
Advancing Tobacco Cessation in LMICs
Authors: Shankar Abhishek , Parascandola Mark , Sakthivel Pirabu , Kaur Jagdish , Saini Deepak , Jayaraj Naveen Prabhu
Published: November 2022

Tobacco kills more than 8 million people worldwide every year. Over 80% of the world’s 1.3 billion tobacco users live in low- and middle-income countries (LMICs), where the future burden is projected to grow. At the same time, progress in tobacco control has not advanced as far as in many LMICs. In particular, the implementation of tobacco-cessation programs and interventions remains limited. The bulk of the evidence for tobacco-cessation interventions comes from high-income countries and may not reflect the context in LMICs, particularly as resources and training for tobacco cessation are limited. This paper summarizes the current evidence for tobacco-cessation interventions in LMICs and highlights some key challenges and research gaps. Overall, there is a need to build capacity for locally relevant research and implementation science to support tailored cessation interventions and strategies for LMICs.
Characteristics of Interval Colorectal Cancer: A Canadian Retrospective Population-Level Analysis from Newfoundland and Labrador
Authors: Shanahan Jessica J. , LeBlanc Danielle M. , Courage Emily R. , Benesch Matthew G. K. , Hickey Kala E. , Hartwig Katia A. , Armstrong Casey D. , Engelbrecht Reniel , Fagan Mitchell G. , Borgaonkar Mark R. , Pace David E.
Published: November 2022

Interval colorectal cancers (I-CRCs) arise during the interval time period between scheduled colonoscopies. Predicting which patients are at risk of I-CRCs remains an elusive undertaking, but evidence would suggest that most I-CRCs arise from lesions missed on index endoscopy. The procedural factors that lead to missed lesions are numerous and lack consensus in the literature. In Canada, the province of Newfoundland and Labrador has the highest incidence of CRCs. In this study our aim was to examine I-CRCs (3–60 months after last colonoscopy) in NL through a population-level analysis covering 67% of the province from 2001–2018. We estimated the I-CRC rate to be up to 9.3%. Median age of I-CRC diagnosis was 67.1 years with an interval time of 2.9 years. About 57% of these tumors occurred proximal to the splenic flexure, with 53% presenting as local disease. No temporal differences were observed in interval time or tumor distribution. On univariate and multivariable logistical regression, risk of right-sided I-CRC did not correlate to the index colonoscopy indication, bowel preparation quality, size of largest polyp removed, colonoscopy completion rate, or stage at presentation. Improvements in synoptic reporting utilization and national registries are needed to identity risk factors and reduce I-CRC frequency.
Effectiveness of Abdominal Ultrasonography for Improving the Prognosis of Pancreatic Cancer during Medical Checkup: A Single Center Retrospective Analysis
Authors: Yamaguchi Atsushi , Kato Naohiro , Sugata Shuhei , Hamada Takuro , Furuya Nao , Mizumoto Takeshi , Tamaru Yuzuru , Kusunoki Ryusaku , Kuwai Toshio , Kouno Hirotaka , Toyota Naoyuki , Sudo Takeshi , Kuraoka Kazuya , Kohno Hiroshi
Published: November 2022

Recent advancements in surgical and anti-cancer therapies have provided significant hope of long survival in patients with pancreatic cancer (PC). To realize this hope, routine medical checkups of asymptomatic people should be performed to identify operable PCs. In this study, we evaluated the efficacy of medical checkups using abdominal ultrasonography (US). We retrospectively analyzed 374 patients with PC at our institute between 2010 and 2021. We divided these patients into several groups according to the diagnostic approach and compared their background and prognosis. These groups comprised PCs diagnosed through (a) symptoms, 242 cases; (b) US during medical checkup for asymptomatic individuals, 17; and other means. Of the 374 patients, 192 were men (51.3%), and the median age was 74 years (34–105). Tumors were located in the pancreatic tail in 67 patients (17.9%). Excision ratio and 5-year survival rate were significantly better in group (b) than in (a) (58.8% vs. 23.1%, p < 0.01 and 42.2% vs. 9.4%, p < 0.001, respectively). The prognosis of patients diagnosed using US during medical checkup was better than that of patients identified through symptomatic presentation of PC. US for asymptomatic individuals with PC might be one of the useful modalities for promoting better prognosis of PCs.