bluuebunny/crossref_metadata_2025 huggingface dataset:
Methionine Promotes Milk Protein Synthesis via the PI3K-mTOR Signaling Pathway in Human Mammary Epithelial Cells
Authors: Li Peizhi , Fang Xibi , Hao Guijie , Li Xiaohui , Cai Yue , Yan Yuhao , Zan Liting , Yang Runjun , Liu Boqun
Published: November 2023

Breast milk is widely considered to be the most natural, safe, and complete food for infants. However, current breastfeeding rates fall short of the recommendations established by the World Health Organization. Despite this, there are few studies that have focused on the promotion of human lactation through nutrient supplementation. Therefore, the aim of this study was to investigate the effect of methionine on milk synthesis in human mammary epithelial cells (MCF-10A cells) and to explore the underlying mechanisms. To achieve this, MCF-10A cells were cultured with varying concentrations of methionine, ranging from 0 to 1.2 mM. Our results indicated that 0.6 mM of methionine significantly promoted the synthesis of milk protein. An RNA-seq analysis revealed that methionine acted through the PI3K pathway. This finding was validated through real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting. In addition, PI3K inhibition assays confirmed that methionine upregulated the expression of both mTOR and p-mTOR through activation of PI3K. Taken together, these findings suggest that methionine positively regulates milk protein synthesis in MCF-10A cells through the PI3K-mTOR signaling pathway.
Fluoropolymers as Unique and Irreplaceable Materials: Challenges and Future Trends in These Specific Per or Poly-Fluoroalkyl Substances
Authors: Améduri Bruno
Published: November 2023

In contrast to some low-molar-mass per- and polyfluoroalkyl substances (PFASs), which are well established to be toxic, persistent, bioaccumulative, and mobile, fluoropolymers (FPs) are water-insoluble, safe, bioinert, and durable. These niche high-performance polymers fulfil the 13 polymer-of-low-concern (PLC) criteria in their recommended conditions of use. In addition, more recent innovations (e.g., the use of non-fluorinated surfactants in aqueous radical (co)polymerization of fluoroalkenes) from industrial manufacturers of FPs are highlighted. This review also aims to show how these specialty polymers endowed with outstanding properties are essential (even irreplaceable, since hydrocarbon polymer alternatives used in similar conditions fail) for our daily life (electronics, energy, optics, internet of things, transportation, etc.) and constitute a special family separate from other “conventional” C1–C10 PFASs found everywhere on Earth and its oceans. Furthermore, some information reports on their recycling (e.g., the unzipping depolymerization of polytetrafluoroethylene, PTFE, into TFE), end-of-life FPs, and their risk assessment, circular economy, and regulations. Various studies are devoted to environments involving FPs, though they present a niche volume (with a yearly production of 330,300 t) compared to all plastics (with 460 million t). Complementary to other reviews on PFASs, which lack of such above data, this review presents both fundamental and applied strategies as evidenced by major FP producers.
Stereocontrolled Synthesis and Conformational Analysis of a Series of Disaccharides α,β-d-GlcA-(1→3)-α-L-Fuc
Authors: Gerbst Alexey G. , Vinnitsky Dmitry Z. , Tokatly Alexandra I. , Dmitrenok Andrey S. , Krylov Vadim B. , Ustuzhanina Nadezhda E. , Nifantiev Nikolay E.
Published: November 2023

D-Glucuronic acid is a fundamental building block of many biologically important polysaccharides, either in its non-substituted form or bearing a variety of substituents, among them sulfates. We have previously performed a study of the effects of exhaustive sulfation on the conformational behavior of β-gluronopyranosides. Herein, we report an investigation comparing α- and β-derivatives of this monosaccharide within the title disaccharides using NMR and quantum chemistry approaches. It was found that for α-linked disaccharides, the introduction of sulfates did not greatly affect their conformational behavior. However, for β-derivatives, considerable conformational changes were observed. In general, they resemble those that took place for the monosaccharides, except that NOESY experiments and calculations of intra-ring spin–spin coupling constants suggest the presence of a 1S5 conformer along with 3S1 in the fully sulfated disaccharide. During the synthesis of model compounds, hydrogen bond-mediated aglycone delivery was used as an α-directing stereocontrol approach in the glucuronidation reaction.
Stevia Polyphenols, Their Antimicrobial and Anti-Inflammatory Properties, and Inhibitory Effect on Digestive Enzymes
Authors: Myint Khaing Zar , Zhou Zhuoyu , Shi Qiandai , Chen Junming , Dong Xinyu , Xia Yongmei
Published: November 2023

Polyphenols from stevia leaves (PPSs) are abundant byproducts from steviol glycoside production, which have been often studied as raw extracts from stevia extracts for their bioactivities. Herein, the PPSs rich in isochlorogenic acids were studied for their antimicrobial and anti-inflammatory properties, as well as their inhibitory effects on digestive enzymes. The PPSs presented stronger antibacterial activity against E. coli, S. aureus, P. aeruginosa, and B. subtilis than their antifungal activity against M. furfur and A. niger. Meanwhile, the PPSs inhibited four cancer cells by more than 60% based on their viability, in a dose-dependent manner. The PPSs presented similar IC50 values on the inhibition of digestive enzyme activities compared to epigallocatechin gallate (EGCG), but had weaker anti-inflammatory activity. Therefore, PPSs could be a potential natural alternative to antimicrobial agents. This is the first report on the bioactivity of polyphenols from stevia rebaudiana (Bertoni) leaves excluding flavonoids, and will be of benefit for understanding the role of PPSs and their application.
Characterization of Molecular Interactions in the Bondline of Composites from Plasma-Treated Aluminum and Wood
Authors: Zimmermann Sascha Jan , Moritz Philipp , Höfft Oliver , Wegewitz Lienhard , Maus-Friedrichs Wolfgang , Dahle Sebastian
Published: November 2023

Wood and aluminum composites are becoming increasingly attractive due to their ability to combine the advantages of both materials: the lightweight nature of wood and the strength of aluminum. However, using conventional wood adhesives like polyvinyl acetate (PVAc) to bond these dissimilar materials is challenging and requires special surface treatments. Prior studies have demonstrated that applying a dielectric barrier discharge plasma treatment significantly enhances shear and bending strengths in beech wood/aluminum bonds. This study focuses on the molecular interactions between PVAc and aluminum or beech wood influenced by plasma surface modification. Surface-sensitive methods, including X-ray photoelectron spectroscopy, infrared reflection adsorption spectroscopy and atomic force microscopy, were employed to characterize the PVAc films on the corresponding surfaces and to identify possible interactions. The ultrathin PVAc films required for this purpose were deposited by spin coating on untreated and plasma-treated aluminum. The aluminum surface was cleaned and oxidized by plasma. Additionally, hydroxyl species could be detected on the surface. This can lead to the formation of hydrogen bonds between the aluminum and the carbonyl oxygen of PVAc after plasma treatment, presumably resulting in increased bond strength. Furthermore, the beech wood surface is activated with polar oxygen species.
Metabolic Transformation of Gentiopicrin, a Liver Protective Active Ingredient, Based on Intestinal Bacteria
Authors: Fu Jie , Yu Hang , Guo Qinglan , Wang Yanan , Xu Hui , Lu Jinyue , Hu Jiachun , Wang Yan
Published: November 2023

Gentiopicrin, the main component of the famous Chinese patent medicine Long Dan Xie Gan Wan, has the characteristics of fast absorption in vivo and low bioavailability. Intestinal bacteria play an important role in the absorption and pharmacokinetics of oral drugs. In this study, the metabolic transformation of gentiopicrin by intestinal bacteria was examined. High-performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry (LC/MSn-IT-TOF) and nuclear magnetic resonance (NMR) were used, and six metabolites were identified, including reduction products (G-M1, G-M2, G-M4, and G-M6), a hydrolytic product (G-M3), and a dehydration product (G-M5) of gentiopicrin aglycone after hydrolysis, reduction, and dehydration reactions were performed by the intestinal flora. This is the first time that chiral metabolites of gentiopicrin (G-M1 and G-M2) were found in this study. In addition, the precursors of glucuronic acid conjugates previously reported in vivo may have come from the intestinal bacterial metabolites G-M1, G-M2, and G-M3. In addition, the metabolic transformation of gentiopicrin in liver microsomes was studied in vitro, and it was found that gentiopicrin did not undergo metabolic transformation under the action of liver microsomes. It is suggested that gentiopicroside may be metabolized in the intestine. This study provides both new insight regarding the investigation of effective substances and an exploration of the pharmacodynamic and toxicological properties of gentiopicrin.
Coagulation of Hydrophobic Ionic Associates of Cetyltrimethylammonium Bromide and Carrageenan
Authors: Shyichuk Alexander , Ziółkowska Dorota , Szulc Joanna
Published: November 2023

In aqueous solutions, cetyltrimethylammonium cations bind to carrageenan polyanions, and the resulting ionic associates form macroscopic aggregates due to hydrophobic interaction. At certain ratios of cetyltrimethylammonium to carrageenan, the resulting colloidal particles auto-flocculate. According to visual observations, the ratio ranges from 1 to 3 mmol/g; otherwise the suspensions are stable. By measuring the sedimentation rate and particle size distribution, the most extensive flocculation was found to be from 1.7 to 2.3 mmol/g. The ratio corresponding to the fastest auto-flocculation was precisely determined by titrating the reagents with small increments and recording the turbidity. The turbidimetric titration plots contain distinct break points corresponding to the most extensive flocculation. These break points occur at the same ratio of carrageenan to cetyltrimethylammonium over a wide range of reagent concentrations. The precise values of the critical ratio were found to be 1.78 and 1.53 mmol/g, respectively, during the titration of cetyltrimethylammonium with carrageenan and vice versa. The number of anionic sulfate groups in carrageenan was measured by ICP OES and found to be 1.35 mmol/g. This value is consistent with the critical ratio of the auto-flocculation.
The Synthesis, Structure, and Dielectric Properties of a One-Dimensional Hydrogen-Bonded DL-α-Phenylglycine Supramolecular Crown-Ether-Based Inclusion Compound
Authors: Liu Yang , Hu Hongzhi , Qi Huanhuan , Lv Meixia , Liu Zunqi
Published: November 2023

A novel hydrogen-bonded supramolecular crown-ether-based inclusion compound, [(DL-α-Phenylglycine)(18-crown-6)]+[(CoCl4)0.5]−(1), was obtained via evaporation in a methanolic solution at room temperature using DL-α-phenylglycine, 18-crown-6, cobalt chloride (CoCl2), and hydrochloric acid. Its structure, thermal properties, and electrical properties were characterized via elemental analysis, single-crystal X-ray diffraction, variable-temperature infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, and variable temperature–variable frequency dielectric constant testing. The compound was a monoclinic crystal system in the C2 space group at low temperature (100 K) and room temperature (293 K). Analysis of the single crystal structure showed that [(CoCl4)0.5]− presented an edge-sharing ditetrahedral structure in the disordered state, while the protonated DL-α-phenylglycine molecule in the disordered state and intramolecular hydroxyl group (-OH) underwent dynamic rocking, causing a significant stretching motion of the O-H···Cl-type one-dimensional hydrogen bond chain. This resulted in dielectric anomalies in the three axes of the crystal, thus showing significant dielectric anisotropy.
How to Target Small-Molecule Fluorescent Imaging Probes to the Plasma Membrane—The Influence and QSAR Modelling of Amphiphilicity, Lipophilicity, and Flip-Flop
Authors: Horobin Richard W. , Stockert Juan C.
Published: November 2023

Many new fluorescent probes targeting the plasma membrane (PM) of living cells are currently being described. Such probes are carefully designed to report on relevant membrane features, but oddly, the structural features required for effective and selective targeting of PM often receive less attention, constituting a lacuna in the molecular design process. We aim to rectify this by clarifying how the amphiphilicity and lipophilicity of a probe, together with the tendency to flip-flop across the membrane, contribute to selective PM accumulation. A simplistic decision-rule QSAR model has been devised that predicts the accumulation/non-accumulation of small-molecule fluorescent probes in the PM. The model was based on probe log P plus various derived measures, allowing the roles of amphiphilicity, lipophilicity, and flip-flop to be taken into account. The validity and wide applicability of the model were demonstrated by evaluating its ability to predict amphiphilicity or PM accumulation patterns in surfactants, drugs, saponins, and PM probes. It is hoped that the model will aid in the more efficient design of effective PM probes.
2,5-[C4+C2] Ringtransformation of Pyrylium Salts with α-Sulfinylacetaldehydes
Authors: Bauer Dominik , Hofmann Kathrin , Reggelin Michael
Published: November 2023

A rapid synthesis of chiral sulfoxide-functionalized meta-terphenyl derivatives by a 2,5-[C4+C2] ring transformation reaction of pyrylium salts with in situ generated enantiomerically pure α-sulfinylacetaldehydes is described in this paper. This synthetic method demonstrates, for the first time, the use of α-sulfinylacetaldehydes in a reaction sequence initiated by the nucleophilic attack of pyrylium salts by α-sulfinylcarbanions to generate chiral aromatic systems. The method presented shows a broad applicability starting with various methyl sulfoxides and a number of functionalized pyrylium salts, furnishing meta-terphenyls with complex substitution patterns from readily accessible starting compounds.
BILFF: All-Atom Force Field for Modeling Triazolium- and Benzoate-Based Ionic Liquids
Authors: Roos Eliane , Sebastiani Daniel , Brehm Martin
Published: November 2023

We present an extension of our previously developed all-atom force field BILFF (Bio-polymers in Ionic Liquids Force Field) to three different ionic liquids: 1-ethyl-3-methyl-1,2,3-triazolium acetate ([EMTr][OAc]), 1-ethyl-3-methyl-1,2,3-triazolium benzoate ([EMTr][OBz]), and 1-ethyl-3-methylimidazolium benzoate ([EMIm][OBz]). These ionic liquids are of practical importance as they have the ability to dissolve significant amounts of cellulose even at room temperature. Our force field is optimized to accurately reproduce the strong hydrogen bonding in the system with nearly quantum chemical accuracy. A very good agreement between the microstructure of the quantum chemical simulations over a wide temperature range and experimental density data with the results of BILFF were observed. Non-trivial effects, such as the solvation shell structure and π–π stacking of the cations, are also accurately reproduced. Our force field enables accurate simulations of larger systems, such as solvated cellulose in different (aqueous) ionic liquids, and is the first to present the optimized parameters for mixtures of these solvents and water.
Study on the Pore Structure of Lightweight Mortar with Nano-Additives
Authors: Du Yiying , Pundienė Ina , Pranckevičienė Jolanta , Zujevs Aleksejs , Korjakins Aleksandrs
Published: November 2023

With the development of nanotechnology, nanomaterials have been introduced to improve the engineering properties of cement-based building materials. An abundant number of studies have been carried out on normal-weight concrete using multi-walled carbon nanotubes (MWCNTs) or nano-silica (NS) and have proven their effectiveness. Nevertheless, still very few investigations are available in terms of lightweight cement-based materials, especially when MWCNTs and NS are binarily incorporated. Thus, in this study, fly ash cenospheres (FACs) according to cement weight were applied as lightweight fine aggregates to produce lightweight mortar (LWM). MWCNTs at 0.05, 0.15, and 0.45% and NS at 0.2 and 1.0% were binarily added as modifiers. Compressive and flexural strengths were tested to investigate mechanical behaviors. A water absorption test was conducted, together with scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP), to identify the impacts of the nano-additives on the pore structure of LWM. The following results were obtained: MWCNTs and NS demonstrated synergic effects on enhancing the mechanical properties of LWM. MWCNTs exerted positive impacts on reducing the porosity and improving the pore distribution at low dosages of 0.05 and 0.15%. The hybrid addition of NS further transformed large voids into small ones and introduced closed pores.
Nanocomposite Photoanisotropic Materials for Applications in Polarization Holography and Photonics
Authors: Nazarova Dimana , Nedelchev Lian , Berberova-Buhova Nataliya , Mateev Georgi
Published: November 2023

Photoanisotropic materials, in particular azodyes and azopolymers, have attracted significant research interest in the last decades. This is due to their applications in polarization holography and 4G optics, enabling polarization-selective diffractive optical elements with unique properties, including circular polarization beam-splitters, polarization-selective bifocal lenses, and many others. Numerous methods have been applied to increase the photoinduced birefringence of these materials, and as a result, to obtain polarization holographic elements with a high diffraction efficiency. Recently, a new approach has emerged that has been extensively studied by many research groups, namely doping azobenzene-containing materials with nanoparticles with various compositions, sizes, and morphologies. The resulting nanocomposites have shown significant enhancement in their photoanisotropic response, including increased photoinduced birefringence, leading to a higher diffraction efficiency and a larger surface relief modulation in the case of polarization holographic recordings. This review aims to cover the most important achievements in this new but fast-growing field of research and to present an extensive comparative analysis of the result, reported by many research groups during the last two decades. Different hypotheses to explain the mechanism of photoanisotropy enhancement in these nanocomposites are also discussed. Finally, we present our vision for the future development of this scientific field and outline its potential applications in advanced photonics technologies.
Identification of Crocetin as a Dual Agonist of GPR40 and GPR120 Responsible for the Antidiabetic Effect of Saffron
Authors: Zhao Xiaodi , Ahn Dohee , Nam Gibeom , Kwon Jihee , Song Songyi , Kang Min Ji , Ahn Hyejin , Chung Sang J.
Published: November 2023

Crocin, a glycoside of crocetin, has been known as the principal component responsible for saffron’s antidiabetic, anticancer, and anti-inflammatory effects. Crocetin, originating from the hydrolytic cleavage of crocin in biological systems, was subjected to ligand-based virtual screening in this investigation. Subsequent biochemical analysis unveiled crocetin, not crocin, as a novel dual GPR40 and GPR120 agonist, demonstrating a marked preference for GPR40 and GPR120 over peroxisome proliferator-activated receptors (PPAR)γ. This compound notably enhanced insulin and GLP-1 secretion from pancreatic β-cells and intestinal neuroendocrine cells, respectively, presenting a dual mechanism of action in glucose-lowering effects. Docking simulations showed that crocetin emulates the binding characteristics of natural ligands through hydrogen bonds and hydrophobic interactions, whereas crocin’s hindered fit within the binding pocket is attributed to steric constraints. Collectively, for the first time, this study unveils crocetin as the true active component of saffron, functioning as a GPR40/120 agonist with potential implications in antidiabetic interventions.
Sleeve Gastrectomy Provides Cardioprotection from Oxidative Stress In Vitro Due to Reduction of Circulating Myeloperoxidase
Authors: Barron Matthew , Hayes Hailey , Bice Zachary , Pritchard Kirkwood , Kindel Tammy Lyn
Published: November 2023

Bariatric surgery, including sleeve gastrectomy (SG), improves systolic and diastolic function, which is independent of weight loss in rodent models. The cause of weight loss-independent improvements in cardiac function are unknown but may originate from the gastrointestinal tract. In this study, we investigated whether a circulating blood factor is a mechanism for acute cardioprotection after SG by testing the utility of rodent SG plasma to reduce metabolic stress in vitro. For the initial experiment, obese male Zucker rats underwent SG, ad lib sham, or pair-fed sham surgeries (n = six SG, n = eight SH, n = eight PF). For all other studies, a second group of Zucker rats underwent SG or ad lib sham surgeries (n = eight SH, n = six SG). Six weeks following surgery, plasma was collected from each group, both in the fasting and post-prandial (pp) state. This plasma was then pooled per surgical group and nutrient state and tested in multiple in vitro cell culture and extra-cellular assays to determine the effect of SG on myotubular metabolic stress compared to the sham surgeries. Post-prandial SG plasma (ppSG), but not fasting SG, pp, or fasting sham plasma, reduced the metabolic stress of the H9c2 cells as measured by lactate dehydrogenase (LDH) release (p < 0.01). Unlike SG, weight reduction through pair-feeding did not prevent H9c2 metabolic stress. The PpSG plasma had the slowest rate of extracellular hydrogen peroxide consumption and peroxidatic activity compared to the pp sham, fasting SG, and fasting sham groups. Redox testing of plasma with aminiobenzoic acid hydrazide and edaravone suggested a pattern supporting myeloperoxidase (MPO), or other peroxidases, as the primary component responsible for reduced metabolic stress with ppSG plasma. The PpSG plasma contained 35% less circulating MPO protein as compared to the pp sham and fasting SG plasma. The plasma from an MPO global knockout rat also prevented metabolic stress of the H9c2 cells, compared to the significant increase in LDH release from the plasma of the WT controls (p < 0.01). The MPO global knockout plasma also had a rate of extracellular hydrogen peroxide consumption and peroxidatic activity comparable to the ppSG plasma. These studies suggest that one of the weight loss-independent mechanisms by which SG improves myocellular function could be a reduced pro-oxidative environment due to lower circulating levels of MPO. It appears that the gastrointestinal tract is of critical importance to these findings, as the MPO levels were only lowered after enteral, nutrient stimulation in the SG rats. If this surgical effect is confirmed in humans, SG may be a unique surgical treatment for multiple diseases with a pathogenesis of inflammation and oxidative damage, including obesity-associated heart failure with preserved ejection fraction.
Long-Term Bovine Colostrum Supplementation in Football Players
Authors: Cieślicka Mirosława , Stankiewicz Błażej , Muszkieta Radosław , Tafil-Klawe Małgorzata , Klawe Jacek , Skarpańska-Stejnborn Anna , Ostapiuk-Karolczuk Joanna
Published: November 2023

Physical exercise, especially of high intensity, is a significant burden on an athlete’s body. It should be emphasized that achieving high results in competitive sports requires the use of significant, sometimes extreme, exercise loads during training, which may result in homeostasis disorders, adversely affecting the fitness of athletes. This study aims to investigate the effects of 6 months of bovine colostrum supplementation on indicators of immune system functioning, selected parameters related to iron management, and anabolic/catabolic balance in young football players. Twenty-eight male football players completed a double-blind, placebo-controlled crossover protocol (24 weeks of colostrum/placebo). A standardized exercise test was executed at the beginning of the trial and after 3 and 6 months of supplementation. Blood samples were taken before and after the exercise test and after 3 h of recovery. Markers of iron homeostasis, pro- and anti-inflammatory balance, and hormonal responses were determined. A significant increase in immunoglobulin G concentration was observed, accompanied by a decrease in inflammatory markers in supplemented athletes. Bovine colostrum supplementation had no significant effect on athletes’ performance or on iron management and hormonal response. The use of bovine colostrum, which is characterized by a high content of immunologically active compounds, can be an element of a relatively mild and safe intervention for reducing inflammation induced by intense physical exercise.
The Novel Tetra-Specific Drug C-192, Conjugated Using UniStac, Alleviates Non-Alcoholic Steatohepatitis in an MCD Diet-Induced Mouse Model
Authors: Kim Jihye , Chang Nakho , Kim Yunki , Lee Jaehyun , Oh Daeseok , Choi Jaeyoung , Kim Onyou , Kim Sujin , Choi Myongho , Lee Junyeob , Lee Junghwa , Kim Jungyul , Cho Minji , Kim Minsu , Lee Kwanghwan , Hwang Dukhyun , Sa Jason K. , Park Sungjin , Baek Seungjae , Im Daeseong
Published: November 2023

Non-alcoholic steatohepatitis (NASH) is a complex disease resulting from chronic liver injury associated with obesity, type 2 diabetes, and inflammation. Recently, the importance of developing multi-target drugs as a strategy to address complex diseases such as NASH has been growing; however, their manufacturing processes remain time- and cost-intensive and inefficient. To overcome these limitations, we developed UniStac, a novel enzyme-mediated conjugation platform for multi-specific drug development. UniStac demonstrated high conjugation yields, optimal thermal stabilities, and robust biological activities. We designed a tetra-specific compound, C-192, targeting glucagon-like peptide 1 (GLP-1), glucagon (GCG), fibroblast growth factor 21 (FGF21), and interleukin-1 receptor antagonist (IL-1RA) simultaneously for the treatment of NASH using UniStac. The biological activity and treatment efficacy of C-192 were confirmed both in vitro and in vivo using a methionine-choline-deficient (MCD) diet-induced mouse model. C-192 exhibited profound therapeutic efficacies compared to conventional drugs, including liraglutide and dulaglutide. C-192 significantly improved alanine transaminase levels, triglyceride accumulation, and the non-alcoholic fatty liver disease activity score. In this study, we demonstrated the feasibility of UniStac in creating multi-specific drugs and confirmed the therapeutic potential of C-192, a drug that integrates multiple mechanisms into a single molecule for the treatment of NASH.
In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections
Authors: Božić Dragana D. , Ćirković Ivana , Milovanović Jovica , Bufan Biljana , Folić Miljan , Savić Vujović Katarina , Pavlović Bojan , Jotić Ana
Published: November 2023

Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.
Influence of the Molar Activity of 203/212Pb-PSC-PEG2-TOC on Somatostatin Receptor Type 2-Binding and Cell Uptake
Authors: Pretze Marc , Michler Enrico , Runge Roswitha , Wetzig Kerstin , Tietze Katja , Brandt Florian , Schultz Michael K. , Kotzerke Jörg
Published: November 2023

(1) Background: In neuroendocrine tumors (NETs), somatostatin receptor subtype 2 is highly expressed, which can be targeted by a radioactive ligand such as [177Lu]Lu-1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴,-tetraacetic acid-[Tyr3,Thr8]-octreotide (177Lu-DOTA-TOC) and, more recently, by a lead specific chelator (PSC) containing 203/212Pb-PSC-PEG2-TOC (PSC-TOC). The molar activity (AM) can play a crucial role in tumor uptake, especially in receptor-mediated uptake, such as in NETs. Therefore, an investigation of the influence of different molar activities of 203/212Pb-PSC-TOC on cell uptake was investigated. (2) Methods: Optimized radiolabeling of 203/212Pb-PSC-TOC was performed with 50 µg of precursor in a NaAc/AcOH buffer at pH 5.3–5.5 within 15–45 min at 95° C. Cell uptake was studied in AR42 J, HEK293 sst2, and ZR75-1 cells. (3) Results: 203/212Pb-PSC-TOC was radiolabeled with high radiochemical purity >95% and high radiochemical yield >95%, with AM ranging from 0.2 to 61.6 MBq/nmol. The cell uptake of 203Pb-PSC-TOC (AM = 38 MBq/nmol) was highest in AR42 J (17.9%), moderate in HEK293 sstr (9.1%) and lowest in ZR75-1 (0.6%). Cell uptake increased with the level of AM. (4) Conclusions: A moderate AM of 15–40 MBq/nmol showed the highest cell uptake. No uptake limitation was found in the first 24–48 h. Further escalation experiments with even higher AM should be performed in the future. It was shown that AM plays an important role because of its direct dependence on the cellular uptake levels, possibly due to less receptor saturation with non-radioactive ligands at higher AM.
Preparation and Photovoltaic Performance of a Composite TiO2 Nanotube Array/Polyaniline UV Photodetector
Authors: Liu Chunlian , Huang Peipei , Wang Wei , Tan Miao , Fu Fangbao , Feng Yunhui
Published: November 2023

TiO2 has great potential for application in UV photodetectors due to its excellent photoelectric response. In this work, composite nanomaterials of TiO2 nanotube arrays (TiO2 NTAs) and polyaniline (PANI) were successfully prepared on titanium sheets using an anodic oxidation electrochemical method. The results showed that the TiO2 NTA/PANI composite materials had excellent UV photosensitivity and responsiveness and good stability and reproducibility. This was mainly attributed to the p–n heterostructure formed inside the TiO2 NTA/PANI composites that hindered the recombination of photogenerated electron–hole pairs and improved their utilization of UV light. This work provides a theoretical basis for the application of metal oxides in UV photodetectors, which is important for the development of UV photodetectors.
Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests
Authors: Lima Tainara de Paula de Lima , Canelas Caio Augusto de Almeida , Dutra Joyce da Cruz Ferraz , Rodrigues Ana Paula Drummond , Brígida Rebecca Thereza Silva Santa , Concha Viktor Oswaldo Cárdenas , da Costa Fernando Augusto Miranda , Passos Marcele Fonseca
Published: November 2023

Tissue engineering is vital in treating injuries and restoring damaged tissues, aiming to accelerate regeneration and optimize the complex healing process. In this study, multizonal scaffolds, designed to mimic tissues with bilayer architecture, were prepared using the rotary jet spinning technique (RJS scaffolds). Polycaprolactone and different concentrations of alginate hydrogel (2, 4, and 6% m/v) were used. The materials were swollen in pracaxi vegetable oil (PO) (Pentaclethra macroloba) and evaluated in terms of surface morphology, wettability, functional groups, thermal behavior, crystallinity, and cytotoxicity. X-ray diffraction (XRD) showed the disappearance of the diffraction peak 2θ = 31.5° for samples from the polycaprolactone/pracaxi/alginate (PCLOA) group, suggesting a reduction of crystallinity according to the presence of PO and semi-crystalline structure. Wettability gradients (0 to 80.91°) were observed according to the deposition layer and hydrogel content. Pore diameters varied between 9.27 μm and 37.57 μm. Molecular interactions with the constituents of the formulation were observed via infrared spectra with Fourier transform (FTIR), and their influence was detected in the reduction of the maximum degradation temperature within the groups of scaffolds (polycaprolactone/alginate (PCLA) and PCLOA) about the control. In vitro tests indicated reduced cell viability in the presence of alginate hydrogel and PO, respectively.
Wall Slip-Free Viscosity Determination of Filled Rubber Compounds Using Steady-State Shear Measurements
Authors: Kleinschmidt Dennis , Brüning Florian , Petzke Jonas
Published: November 2023

The high-pressure capillary rheometer (HPCR) represents a state-of-the-art instrument for the determination of rheological properties for plastics and rubber compounds. Rubber compounds have an increased tendency to exhibit flow anomalies depending on the compound ingredients and the processing parameters. Combined with non-isothermal effects due to dissipative material heating, this causes rheological material measurements and the resulting material parameters derived from them to be affected by errors, since the fundamental analytical and numerical calculation approaches assume isothermal flow and wall adhesion. In this paper, the applicability of the empirical rheological transfer function of the Cox–Merz rule, which establishes a relationship between shear viscosity measured with a HPCR and complex viscosity measured with a closed cavity rheometer (CCR), is investigated. The Cox–Merz relation could not be verified for an unfilled EPDM raw polymer or for filled, practical rubber compounds. Using a closed cavity rheometer, a methodology based on ramp tests is then introduced to collect wall slip-free steady-state shear viscosity data under isothermal conditions. The generated data show high agreement with corrected viscosity data generated using the HPCR, while requiring less measurement effort.
Speaking of God in the Realm of Aesthetics: Religion in Hölderlin
Authors: Deibl Jakob Helmut
Published: November 2023

This article considers the work and reception of Friedrich Hölderlin with regard to the impact of German Idealism on religion. To this end, two questions must be clarified in advance: can Hölderlin, who is known primarily as a poet, also be placed in the context of German Idealism, and does his work have a significant relationship to religion? I argue that both questions should be answered in the affirmative. Ernst Cassirer’s study Hölderlin und der deutsche Idealismus (1918/19) clearly laid the foundation for appreciating Hölderlin’s place within German Idealism, and the question of God is a leitmotif of Hölderlin’s entire oeuvre. I seek to trace Hölderlin’s influence on understanding religion in three steps: First, I want to show that Hölderlin, in a critical continuation of Kant, does not consider religion solely within the matrix of practical reason, but brings into play the dimension of aesthetics. By situating religion in relation to the two focal points of ethics and aesthetics, a fundamental question of the philosophy of religion is addressed. Second, I employ several examples to show the various conceptions of the divine that the poet elucidates and juxtaposes in his work (Christian motifs, Greek mythology, pantheistic concepts, etc.). This leads to a philosophy of religion that is not determined by dogmatic boundaries. Third, I point out how religion plays a major role in the reception of Hölderlin.
An Automatic Deep Learning Bowhead Whale Whistle Recognizing Method Based on Adaptive SWT: Applying to the Beaufort Sea
Authors: Feng Rui , Xu Jian , Jin Kangkang , Xu Luochuan , Liu Yi , Chen Dan , Chen Linglong
Published: November 2023

The bowhead whale is a vital component of the maritime environment. Using deep learning techniques to recognize bowhead whales accurately and efficiently is crucial for their protection. Marine acoustic remote sensing technology is currently an important method to recognize bowhead whales. Adaptive SWT is used to extract the acoustic features of bowhead whales. The CNN-LSTM deep learning model was constructed to recognize bowhead whale voices. Compared to STFT, the adaptive SWT used in this study raises the SCR for the stationary and nonstationary bowhead whale whistles by 88.20% and 92.05%, respectively. Ten-fold cross-validation yields an average recognition accuracy of 92.85%. The method efficiency of this work was further confirmed by the consistency found in the Beaufort Sea recognition results and the fisheries ecological study. The research results in this paper help promote the application of marine acoustic remote sensing technology and the conservation of bowhead whales.
Study on the Impact of Offshore Wind Farms on Surrounding Water Environment in the Yangtze Estuary Based on Remote Sensing
Authors: Cai Lina , Hu Qunfei , Qiu Zhongfeng , Yin Jie , Zhang Yuanzhi , Zhang Xinkai
Published: November 2023

Offshore wind farms (OWFs), built extensively in recent years, induce changes in the surrounding water environment. The changes in the suspended sediment concentration (SSC) and chlorophyll-a concentration (Chl-aC) induced by an OWF in the Yangtze River Estuary were analyzed based on Chinese Gaofen (GF) satellite data. The results show the following: (1) The flow near the wind turbines makes the bottom water surge, driving the sediment to “re-suspend” and be lost, deepening the scour pit around the bottom of the wind turbines, which is known as “self-digging”. The interaction between the pillar of a wind turbine and tidal currents makes hydrodynamic factors more complicated. Blocking by wind turbines promoting the scour of the bottom seabed of the OWF results in speeding up the circulation rate of sediment loss and “re-suspension”, which contributes to the change in the SSC and Chl-aC. This kind of change in sediment transport in estuarine areas due to human construction affects the balance of the ecological environment. Long-term sediment loss around wind turbines also influences the safety of wind turbines. (2) The SSC and Chl-aC are mainly in the range of 200–600 mg/L and 3–7 μg/L, respectively, in the OWF area, higher than the values obtained in surrounding waters. The SSC and Chl-aC downstream of the OWF are higher than those upstream, with differences of 100–300 mg/L and 0.5–2 μg/L. High SSC and Chl-aC “tails” appear downstream of wind turbines, consistent with the direction of local tidal currents, with lengths in the range of 2–4 km. In addition, the water environment in the vicinity of a wind turbine array, with a roughly 2–5 km scope (within 4 km during flooding and around 2.5 km during ebbing approximately) downstream of the wind turbine array, is impacted by the OWF. (3) In order to solve the problem of “self-digging” induced by OWFs, it is suggested that the distance between two wind turbines should be controlled within 2–3.5 km in the main flow direction, promising that the second row of wind turbines will be placed on the suspended sediment deposition belt induced by the first row. In this way, the problems of ecosystem imbalance and tidal current structure change caused by sediment loss because of local scouring can be reduced. Furthermore, mutual compensation between wind turbines can solve the “self-digging” problem to a certain extent and ensure the safety of OWFs.
Investigating the Relationship between Topographic Factors and Vegetation Spatial Patterns in the Alpine Plateau: A Case Study in the Southwestern Tibetan Plateau
Authors: Li Yan , Gong Jie , Zhang Yunxia
Published: November 2023

Vegetation on the Southwestern Tibetan Plateau (SWTP) is critical to ensuring ecological security and promoting regional economic and social development. Here, we explored the relationship between topographic factors (elevation, slope, and aspect) and the spatial patterns in the normalized difference vegetation index (NDVI) in the SWTP over the past 20 years. The results found that the NDVI in the SWTP was primarily influenced by elevation and slope. The regions with significant variations in NDVI were concentrated between 4500 m to 5500 m and slopes ranging from 0° to 15°. Although the influence of aspect on NDVI was small, there was a decreasing trend in NDVI on sunny slopes and an increasing trend on shady slopes. Dominant topographic conditions were identified by considering 230 different combinations of elevation, slope, and aspect. The combination of topographic parameters indicated stronger patterns in NDVI variability, notably within sections of 0°–25°slopes and below 5000 m elevation. These findings highlight the relevance of topography, notably slope and aspect, for vegetation in alpine settings. The information gathered from this study about the prevailing topographic distribution and vegetation growth state in the SWTP can help with future ecological restoration and conservation efforts in the Tibetan Plateau and other comparable regions worldwide.
Design of a Tripod-Shaped Radiator Patch Antenna for Ultra-Wideband Direction Finding
Authors: Youn Sangwoon , Ohm Sungsik , Jang Byung-Jun , Choo Hosung
Published: November 2023

As UWB technology develops and devices become smaller, miniaturization techniques for an array antenna system are required. In addition, more in-depth research is needed for UWB direction-finding techniques using channel impulse response (CIR) data. This paper proposes an ultra-wideband (UWB) antenna using a single-radiator multiple-port (SRMP) design for the direction-finding systems of smart devices. The proposed SRMP antenna was designed using a single tripod-shaped patch that can replace the array system. The tripod-shaped radiator was optimized using the edge shape design function to improve its broadband and mutual coupling characteristics. For performance verification, the proposed antenna was fabricated, and the reflection coefficient, mutual coupling, and radiation patterns were measured in a fully anechoic chamber. The proposed antenna has an operating frequency band of 6.1 GHz (from 5.8 GHz to 11.9 GHz) for port 1 and a measured mutual coupling of −14.8 dB at 8 GHz. The SRMP antenna has measured maximum gains of 3.5 dBi for port 1 and 2.9 dBi for port 2. To examine the direction-finding performance, the fabricated antenna was connected to a circuit module with a DW3000 chip, which is widely employed in commercial mobile UWB systems. The direction of arrival (DoA) results using the measured CIR data show root-mean-square (RMS) errors of 1.57° and 4.58° at distances of 30 cm and 60 cm.
Crack Segmentation Extraction and Parameter Calculation of Asphalt Pavement Based on Image Processing
Authors: Li Zhongbo , Yin Chao , Zhang Xixuan
Published: November 2023

Crack disease is one of the most serious and common diseases in road detection. Traditional manual methods for measuring crack detection can no longer meet the needs of road crack detection. In previous work, the authors proposed a crack detection method for asphalt pavements based on an improved YOLOv5s model, which is a better model for detecting various types of cracks in asphalt pavements. However, most of the current research on automatic pavement crack detection is still focused on crack identification and location stages, which contributes little to practical engineering applications. Based on the shortcomings of the above work, and in order to improve its contribution to practical engineering applications, this paper proposes a method for segmenting and analyzing asphalt pavement cracks and identifying parameters based on image processing. The first step is to extract the crack profile through image grayscale, histogram equalization, segmented linear transformation, median filtering, Sauvola binarization, and the connected domain threshold method. Then, the magnification between the pixel area and the actual area of the calibration object is calculated. The second step is to extract the skeleton from the crack profile images of asphalt pavement using the Zhang–Suen thinning algorithm, followed by removing the burrs of the crack skeleton image using the connected domain threshold method. The final step is to calculate physical parameters, such as the actual area, width, segments, and length of the crack with images obtained from the crack profile and skeleton. The results show that (1) the method of local thresholding and connected domain thresholding can completely filter noise regions under the premise of retaining detailed crack region information. (2) The Zhang–Suen iterative refinement algorithm is faster in extracting the crack skeleton of asphalt pavement, retaining the foreground features of the image better, while the connected-domain thresholding method is able to eliminate the missed isolated noise. (3) In comparison to the manual calibration method, the crack parameter calculation method proposed in this paper can better complete the calculation of crack length, width, and area within an allowable margin of error. On the basis of this research, a windowing system for asphalt pavement crack detection, WSPCD1.0, was developed. It integrates the research results from this paper, facilitating automated detection and parameter output for asphalt pavement cracks.