bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of soluble surfactant in the linear instability of a film coating inside a tube
Authors: Li Sheng , Chen Ya-Zhou , Cheng Ze , Peng Jie
Published: October 2023

This study investigates the linear instability of a thin-film coating inside a rigid tube. The flow is assumed to be inertialess and driven by an axial body force (e.g. gravity), an interfacial shearing force, or their combinations. The interface and the bulk of the film are laden with soluble surfactant. The properties of the soluble surfactant, i.e. solubility, sorption kinetics and bulk diffusivity, modulate the interfacial dynamics of the film. The influence of these properties on the linear instability of the film is comprehensively investigated via long-wave approximation analysis and numerical calculation. Two modes, namely the interface mode and the surfactant mode, are identified to dominate the instability. For a quiescent film, it is found that solubility, sorption kinetics and bulk diffusivity act to improve the uniformity of the surface surfactant and mitigate the stabilizing effect of the Marangoni force. For the film driven by the axial body/interfacial shearing force, the results reveal that solubility plays contrasting roles in the interface mode and the surfactant mode. A window with intermediate solubility is detected where the film can be linearly stabilized. Moreover, sorption kinetics is found to destabilize the perturbations with long wavelength whereas it stabilizes the perturbations with finite wavelength. The bulk diffusivity of the surfactant has a non-monotonic influence on the flow instability, and the film can be relatively stable at both strong and weak diffusivity.
Entropy and fluctuation relations in isotropic turbulence
Authors: Yao Hanxun , Zaki Tamer A. , Meneveau Charles
Published: October 2023

Based on a generalized local Kolmogorov–Hill equation expressing the evolution of kinetic energy integrated over spheres of size

$\ell$

in the inertial range of fluid turbulence, we examine a possible definition of entropy and entropy generation for turbulence. Its measurement from direct numerical simulations in isotropic turbulence leads to confirmation of the validity of the fluctuation relation (FR) from non-equilibrium thermodynamics in the inertial range of turbulent flows. Specifically, the ratio of probability densities of forward and inverse cascade at scale

$\ell$

is shown to follow exponential behaviour with the entropy generation rate if the latter is defined by including an appropriately defined notion of ‘temperature of turbulence’ proportional to the kinetic energy at scale

$\ell$

.
Analytic inclined flow rule for determining granular rheology under strong non-local effects
Authors: Lee Keng-Lin
Published: October 2023

This study theoretically establishes a flow rule for a granular flow down a rough inclined plane, capable of determining granular rheology in the presence of strong non-local effects resulting from grain cooperativity. To describe the non-local rheology, a Landau–Ginzburg model is formulated in terms of the fluidisation parameter represented by the granular inertial number. The exact solutions of the inertial-number field are solved and provide physical insights into the evolution of the internal rheology and the flow arrest process controlled by the flow height. Through asymptotic analysis in the regime dominated by strong non-locality, the exact solutions are further reduced to yield an analytical flow rule for the mean flow velocity. A comparison between the prediction of the flow rule and experimental data from the literature for sand grains determines the underlying rheology law and the relevant rheological parameters. Thus, the proposed flow rule serves as an effective tool for inferring granular rheology from strongly non-local inclined flow data, surpassing the limitations of the classical flow rule deduced from the local rheology framework.
The Conventionality of Real-Valued Quantities
Authors: Bennett Marissa , Miller Michael E.
Published: October 2023

The original architects of the representational theory of measurement interpreted their formalism operationally and explicitly acknowledged that some aspects of their representations are conventional. We argue that the conventional elements of the representations afforded by the theory require careful scrutiny as one moves toward a more metaphysically robust interpretation by showing that there is a sense in which the very number system one uses to represent a physical quantity such as mass or length is conventional. This result undermines inferences which impute structure from the numerical representational structure to the quantity it is used to represent.
Dark Energy or Modified Gravity?
Authors: Smeenk Chris , Weatherall James Owen
Published: October 2023

We consider some of the epistemic benefits of exploring “theory space” in the context of modifications of general relativity with intended applications in cosmology. We show how studying modifications of general relativity can help in assessing the robustness of empirical inferences, particularly in inaccessible regimes. We also discuss challenges to sharply distinguishing apparently distinct directions in theory space.
Compulsory civic duty and turnout: evidence from a natural experiment
Authors: Artes Joaquin , Jurado Ignacio
Published: October 2023

We study the effect of compulsory civic duty on turnout. We take advantage of a natural experiment in Spain, where electoral officers are selected through a lottery and being an electoral officer is compulsory for those drafted. We analyze whether acting as an officer changes turnout and attitudes in subsequent elections. Our findings show that compulsory civic duty has a strong short-term and positive effect on electoral participation, but has no impact on political attitudes. The positive effect of the civic duty treatment, however, fades after a few months. Our results have implications for theories about civic duty and the formation of political habits.
Willing but Unable: Reassessing the Relationship between Racial Group Consciousness and Black Political Participation
Authors: SMITH JASMINE CARRERA , CLEMONS JARED , KRISHNAMURTHY ARVIND , MARTINEZ MIGUEL , MCLAREN LEANN , WHITE ISMAIL K.
Published: October 2023

In this article, we offer a framework for understanding the role that racial group consciousness (RGC) plays in influencing Black Americans’ engagement in costly political action. Attempting to add clarity to decades of inconsistent and at times contradictory findings, we argue that the effect of RGC at inspiring political action among Black Americans is conditional on (1) the relevance of the political activity to achieving a well-recognized racial group outcome and (2) individual capacity to assume the cost of engaging in the activity. Analyzing data from the ANES and two behavioral experiments, we find that RGC exhibits a consistently strong relationship with engagement in low-cost political behavior, regardless of whether the behavior has some explicit group-relevant outcome. When engagement becomes more costly, however, Blacks high in RGC are only willing to assume these costs if the engagement has some clear potential for racial group benefit.
Burstein’s permutation conjecture, Hong and Li’s inversion sequence conjecture and restricted Eulerian distributions
Authors: Chern Shane , Fu Shishuo , Lin Zhicong
Published: October 2023

Recently, Hong and Li launched a systematic study of length-four pattern avoidance in inversion sequences, and in particular, they conjectured that the number of 0021-avoiding inversion sequences can be enumerated by the OEIS entry A218225. Meanwhile, Burstein suggested that the same sequence might also count three sets of pattern-restricted permutations. The objective of this paper is not only a confirmation of Hong and Li’s conjecture and Burstein’s first conjecture but also two more delicate generating function identities with the $\mathsf{ides}$ statistic concerned in the restricted permutation case and the $\mathsf{asc}$ statistic concerned in the restricted inversion sequence case, which yield a new equidistribution result.
Women's Work and the Occupational Structure in Late Nineteenth-Century Sweden
Authors: Lindström Jonas , Ågren Maria
Published: October 2023

It has long been recognized that, in order to understand economies in the past, we need better information about women's work and tertiary sector work. It is also well known that, while valuable in many ways, nineteenth-century censuses give incomplete information about women's contributions to the economy. Consequently, censuses are a poor basis for estimating the occupational structure. This article offers a solution to these problems by triangulating census data with qualitative information extracted from court records. The result is a more reasonable estimate of the first-level occupational structure in a Swedish local society (Västerås and its surroundings) around 1880. This estimate suggests that just before the onset of industrialization, around eighty per cent of the adult population, women and men, were active in primary and tertiary sector work. Compared to the census, the analysis sets women's share in the primary and the tertiary sectors at higher levels. The article has a strong methodological focus and describes in detail how the court records were analysed and adjusted to be comparable with the census.
‘A firm foundation for future understanding, respect and friendship’: the ideals and reality of post-war town twinning, 1945–2020
Authors: Warwick Tosh
Published: October 2023

Formalized in the 1970s through the Middlesbrough–Oberhausen Town Twinning partnership (Partnerschaft Oberhausen–Middlesbrough), the connection between the two post-industrial towns dates back further to informal connections in the early 1950s and an age of reconciliation between the two nations. This article explores the origins, mechanisms, benefits and challenges of town twinning by drawing upon a rich body of empirical evidence from local authority records, press coverage, interviews and community reminiscence. The study provides the first academic analysis of the changes, challenges, continuities and continued relevance of town twinning in one of Britain’s leading pro-Brexit areas.
Is the emergency department used as a substitute or a complement to primary care in Medicaid?
Authors: Denham Alina , Hill Elaine L. , Raven Maria , Mendoza Michael , Raz Mical , Veazie Peter J.
Published: October 2023

Policies to decrease low-acuity emergency department (ED) use have traditionally assumed that EDs are a substitute for unavailable primary care (PC). However, such policies can exacerbate ED overcrowding, rather than ameliorate it, if patients use EDs to complement, rather than substitute, their PC use. We tested whether Medicaid managed care enrolees visit the ED for nonemergent and PC treatable conditions to substitute for or to complement PC. Based on consumer choice theory, we modelled county-level monthly ED visit rate as a function of PC supply and used 2012–2015 New York Statewide Planning and Research Cooperative System (SPARCS) outpatient data and non-linear least squares method to test substitution vs complementarity. In the post-Medicaid expansion period (2014–2015), ED and PC are substitutes state-wide, but are complements in highly urban and poorer counties during nights and weekends. There is no evidence of complementarity before the expansion (2012–2013). Analyses by PC provider demonstrate that the relationship between ED and PC differs depending on whether PC is provided by physicians or advanced practice providers. Policies to reduce low-acuity ED use via improved PC access in Medicaid are likely to be most effective if they focus on increasing actual appointment availability, ideally by physicians, in areas with low PC provider supply. Different aspects of PC access may be differently related to low-acuity ED use.
The Limits of Deduction in the Identification of Customary International Law
Authors: LANDO Massimo Fabio
Published: October 2023

Much scholarship on customary international law has examined the merits of induction, deduction, and assertion as approaches to custom identification. Save for where international tribunals identify custom by assertion, writers have viewed custom identification that does not rely on evidence of State practice and opinio juris as an example of deductive reasoning. However, writers have stated that, at best, deduction is reasoning from the general to the particular. This article draws on legal philosophy to define the contours of deductive reasoning and argues that pure deduction, namely deduction not combined with other forms of reasoning, is an unsound approach to custom identification. This argument is tested by reference to cases of custom identification by the International Court of Justice, categorised according to three types of deduction: normative, functional, and analogical. This article also explores the authority and utility of custom identification by pure deduction and its impact on content determination.
Underutilization of guideline-recommended therapy in patients 80	years and older with peripheral artery diseases
Authors: Messiha Daniel , Petrikhovich Olga , Lortz Julia , Pinsdorf David , Hogrebe Kristina , Knuschke Ramtin , Hering Ramona , Schulz Mandy , Rassaf Tienush , Rammos Christos
Published: October 2023

Summary: Background: Ageing is a major cardiovascular risk factor with detrimental changes that culminate in a high atherosclerotic burden. Peripheral artery disease (PAD) is a major manifestation of atherosclerosis with high mortality. Guideline-recommended treatment is essential, however implementation is inadequate. With an ageing society, age-related inequalities are important and have not been elucidated in a high-risk PAD population on a nation-wide scale. We sought to analyse outpatient treatment structures and guideline adherence in treatment of PAD patients older than 80 years. Patients and methods: The study is based on ambulatory claims data comprising 70.1 million statutorily insured patients per year in Germany from 2009 to 2018. We analysed age-related differences in prevalence, pharmacotherapy and specialized outpatient care in PAD patients. Results: Of 17,633,970 PAD patients included, 28% were older than 80 years. PAD prevalence increased between 2008 and 2018 (1.85% vs. 3.14%), with the proportion of older patients increasing by a third (24.4% vs. 31.2%). Octogenarians were undertreated regarding guideline-recommended statin pharmacotherapy compared to younger patients while antiplatelets were prescribed more often (statins 2016: 46.5% vs. 52.4%; antiplatelets 2016 30.6% vs. 29.3%; p<.05). Furthermore, octogenarians received less specialized outpatient care (angiology: 6.4% vs. 9.5%, vascular surgery: 8.1% vs. 11.8%, cardiology: 25.2% vs. 29.2%, p<.05). Conclusions: Our results demonstrate that age-related differences in pharmacotherapy and specialized outpatient care of PAD patients are evident. While overall guideline-recommended outpatient treatment is low, patients 80 years and older are less likely to receive both, leaving age-related health inequalities a challenge of our future.
Modeling Snow and Ice Microwave Emissions in the Arctic for a Multi‐Parameter Retrieval of Surface and Atmospheric Variables From Microwave Radiometer Satellite Data
Authors: Rückert Janna E. , Huntemann Marcus , Tonboe Rasmus Tage , Spreen Gunnar
Published: October 2023

Monitoring surface and atmospheric parameters—like water vapor—is challenging in the Arctic, despite the daily Arctic‐wide coverage of spaceborne microwave radiometer data. This is mainly due to the difficulties in characterizing the sea ice surface emission: sea ice and snow microwave emission is high and highly variable. There are very few data sets combining relevant in situ measurements with co‐located remote sensing data, which further complicates the development of accurate retrieval algorithms. Here, we present a multi‐parameter retrieval based on the inversion of a forward model for both, atmosphere and surface, for non‐melting conditions. The model consists of a layered microwave emission model of snow and ice. Since snow scattering and emission effects, as well as temperature gradients, are taken into account, a high variability in brightness temperatures can be simulated. For ocean regions and the atmosphere existing parameterized forward models are used. By using optimal estimation, the forward model can be inverted allowing for the simultaneous and consistent retrieval of nine variables: integrated water vapor, liquid water path, sea ice concentration, multi‐year ice fraction, snow depth, snow‐ice interface temperature and snow‐air interface temperature as well as sea‐surface temperature and wind speed (over open ocean). In addition, the method provides retrieval uncertainty estimates for each retrieved parameter. To evaluate the forward model as well as the retrieval, we use the extensive data sets acquired during the year‐long Arctic expedition Multidisciplinary drifting Observatory for the Study of Arctic Climate (2019–2020) as a reference.
Twenty-four-hour ambulatory, but not clinic blood pressure associates with leptin in young adults with overweight or obesity: The African-PREDICT study
Authors: van Niekerk Elandi , Botha-Le Roux Shani , Mels Catharina M. C. , Swanepoel Mariette , Delles Christian , Welsh Paul , Kruger Ruan
Published: October 2023

Hypertension and obesity are known pro-inflammatory conditions, and limited studies explored various blood pressure modalities and inflammatory markers in young adults with overweight or obesity (OW/OB). We assessed the relationship of clinic and 24 h ambulatory blood pressure with an array of inflammatory markers in young adults with OW/OB. This cross-sectional study included women and men of Black and White ethnicity (n = 1194) with a median age of 24.5 ± 3.12 years. Participants were divided into normal weight and OW/OB groups according to body mass index. Clinic and 24 h ambulatory systolic and diastolic blood pressure were measured. Inflammatory markers included leptin, interleukin-6, interleukin-8, tumour necrosis factor-α, adiponectin, interleukin-10, and C-reactive protein. After adjustments for age, sex, and ethnicity, the OW/OB group had higher blood pressure and an overall worse inflammatory profile compared to the normal weight group (all p ≤ 0.024). In the OW/OB group, 24 h systolic (r = 0.22; p < 0.001) and diastolic blood pressure (r = 0.28; p < 0.001) correlated with leptin, independent of age, sex, and ethnicity. In fully adjusted regression models, 24 h systolic blood pressure (adj.R2 = 0.25; β = 0.28; p = 0.035) and diastolic blood pressure (adj.R2 = 0.10; β = 0.32; p = 0.034), associated with leptin in the OW/OB group and significance remained with additional adjustments for visceral adiposity index. Twenty-four-hour ambulatory, but not clinic blood pressure, is related to leptin in young adults with OW/OB. Leptin shows a stronger relationship with adiposity when compared to other inflammatory markers and may play a role in subcutaneous adiposity-related increased blood pressure.
Immune checkpoint inhibitor-induced colitis is mediated by polyfunctional lymphocytes and is dependent on an IL23/IFNγ axis
Authors: Lo Jonathan W. , Cozzetto Domenico , Alexander James L. , Danckert Nathan P. , Madgwick Matthew , Knox Naomi , Sieh Jillian Yong Xin , Olbei Marton , Liu Zhigang , Ibraheim Hajir , Blanco Jesus Miguens , Kudo Hiromi , Seoane Rocio Castro , Possamai Lucia A. , Goldin Robert , Marchesi Julian , Korcsmaros Tamas , Lord Graham M. , Powell Nick
Published: October 2023

Immune checkpoint inhibitors (CPIs) are a relatively newly licenced cancer treatment, which make a once previously untreatable disease now amenable to a potential cure. Combination regimens of anti-CTLA4 and anti-PD-1 show enhanced efficacy but are prone to off-target immune-mediated tissue injury, particularly at the barrier surfaces. To probe the impact of immune checkpoints on intestinal homoeostasis, mice are challenged with anti-CTLA4 and anti-PD-1 immunotherapy and manipulation of the intestinal microbiota. The immune profile of the colon of these mice with CPI-colitis is analysed using bulk RNA sequencing, single-cell RNA sequencing and flow cytometry. CPI-colitis in mice is dependent on the composition of the intestinal microbiota and by the induction of lymphocytes expressing interferon-γ (IFNγ), cytotoxicity molecules and other pro-inflammatory cytokines/chemokines. This pre-clinical model of CPI-colitis could be attenuated following blockade of the IL23/IFNγ axis. Therapeutic targeting of IFNγ-producing lymphocytes or regulatory networks, may hold the key to reversing CPI-colitis.
Genome-wide association study of thyroid-stimulating hormone highlights new genes, pathways and associations with thyroid disease
Authors: Williams Alexander T. , Chen Jing , Coley Kayesha , Batini Chiara , Izquierdo Abril , Packer Richard , Abner Erik , Kanoni Stavroula , Shepherd David J. , Free Robert C. , Hollox Edward J. , Brunskill Nigel J. , Ntalla Ioanna , Reeve Nicola , Brightling Christopher E. , Venn Laura , Adams Emma , Bee Catherine , Wallace Susan E. , Pareek Manish , Hansell Anna L. , Esko Tõnu ,   Estonian Biobank Research Team, Stow Daniel , Jacobs Benjamin M. , van Heel David A. ,   Genes & Health Research Team, Hennah William , Rao Balasubramanya S. , Dudbridge Frank , Wain Louise V. , Shrine Nick , Tobin Martin D. , John Catherine
Published: October 2023

Thyroid hormones play a critical role in regulation of multiple physiological functions and thyroid dysfunction is associated with substantial morbidity. Here, we use electronic health records to undertake a genome-wide association study of thyroid-stimulating hormone (TSH) levels, with a total sample size of 247,107. We identify 158 novel genetic associations, more than doubling the number of known associations with TSH, and implicate 112 putative causal genes, of which 76 are not previously implicated. A polygenic score for TSH is associated with TSH levels in African, South Asian, East Asian, Middle Eastern and admixed American ancestries, and associated with hypothyroidism and other thyroid disease in South Asians. In Europeans, the TSH polygenic score is associated with thyroid disease, including thyroid cancer and age-of-onset of hypothyroidism and hyperthyroidism. We develop pathway-specific genetic risk scores for TSH levels and use these in phenome-wide association studies to identify potential consequences of pathway perturbation. Together, these findings demonstrate the potential utility of genetic associations to inform future therapeutics and risk prediction for thyroid diseases.
Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach
Authors: Noszka Mateusz , Strzałka Agnieszka , Muraszko Jakub , Kolenda Rafał , Meng Chen , Ludwig Christina , Stingl Kerstin , Zawilak-Pawlik Anna
Published: October 2023

The gastric human pathogenHelicobacter pylorihas developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicate that HP1021 modulatesH. pylori’sresponse to oxidative stress. HP1021 controls the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins are differently expressed in the HP1021 deletion mutant. HP1021 controls typical ROS response pathways (katA,rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. HP1021 is a molecular regulator of competence inH. pylori, as HP1021-dependent repression of thecomBDNA uptake genes is relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controls glucose consumption by directly regulating thegluPtransporter and has an important impact on maintaining the energetic balance in the cell.
Hierarchical organization and assembly of the archaeal cell sheath from an amyloid-like protein
Authors: Wang Hui , Zhang Jiayan , Toso Daniel , Liao Shiqing , Sedighian Farzaneh , Gunsalus Robert , Zhou Z. Hong
Published: October 2023

Certain archaeal cells possess external proteinaceous sheath, whose structure and organization are both unknown. By cellular cryogenic electron tomography (cryoET), here we have determined sheath organization of the prototypical archaeon, Methanospirillum hungatei. Fitting of Alphafold-predicted model of the sheath protein (SH) monomer into the 7.9 Å-resolution structure reveals that the sheath cylinder consists of axially stacked β-hoops, each of which is comprised of two to six 400 nm-diameter rings of β-strand arches (β-rings). With both similarities to and differences from amyloid cross-β fibril architecture, each β-ring contains two giant β-sheets contributed by ~ 450 SH monomers that entirely encircle the outer circumference of the cell. Tomograms of immature cells suggest models of sheath biogenesis: oligomerization of SH monomers into β-ring precursors after their membrane-proximal cytoplasmic synthesis, followed by translocation through the unplugged end of a dividing cell, and insertion of nascent β-hoops into the immature sheath cylinder at the junction of two daughter cells.
DNMT and HDAC inhibition induces immunogenic neoantigens from human endogenous retroviral element-derived transcripts
Authors: Goyal Ashish , Bauer Jens , Hey Joschka , Papageorgiou Dimitris N. , Stepanova Ekaterina , Daskalakis Michael , Scheid Jonas , Dubbelaar Marissa , Klimovich Boris , Schwarz Dominic , Märklin Melanie , Roerden Malte , Lin Yu-Yu , Ma Tobias , Mücke Oliver , Rammensee Hans-Georg , Lübbert Michael , Loayza-Puch Fabricio , Krijgsveld Jeroen , Walz Juliane S. , Plass Christoph
Published: October 2023

Immunotherapies targeting cancer-specific neoantigens have revolutionized the treatment of cancer patients. Recent evidence suggests that epigenetic therapies synergize with immunotherapies, mediated by the de-repression of endogenous retroviral element (ERV)-encoded promoters, and the initiation of transcription. Here, we use deep RNA sequencing from cancer cell lines treated with DNA methyltransferase inhibitor (DNMTi) and/or Histone deacetylase inhibitor (HDACi), to assemble a de novo transcriptome and identify several thousand ERV-derived, treatment-induced novel polyadenylated transcripts (TINPATs). Using immunopeptidomics, we demonstrate the human leukocyte antigen (HLA) presentation of 45 spectra-validated treatment-induced neopeptides (t-neopeptides) arising from TINPATs. We illustrate the potential of the identified t-neopeptides to elicit a T-cell response to effectively target cancer cells. We further verify the presence of t-neopeptides in AML patient samples after in vivo treatment with the DNMT inhibitor Decitabine. Our findings highlight the potential of ERV-derived neoantigens in epigenetic and immune therapies.
Telomouse—a mouse model with human-length telomeres generated by a single amino acid change in RTEL1
Authors: Smoom Riham , May Catherine Lee , Ortiz Vivian , Tigue Mark , Kolev Hannah M. , Rowe Melissa , Reizel Yitzhak , Morgan Ashleigh , Egyes Nachshon , Lichtental Dan , Skordalakes Emmanuel , Kaestner Klaus H. , Tzfati Yehuda
Published: October 2023

Telomeres, the ends of eukaryotic chromosomes, protect genome integrity and enable cell proliferation. Maintaining optimal telomere length in the germline and throughout life limits the risk of cancer and enables healthy aging. Telomeres in the house mouse, Mus musculus, are about five times longer than human telomeres, limiting the use of this common laboratory animal for studying the contribution of telomere biology to aging and cancer. We identified a key amino acid variation in the helicase RTEL1, naturally occurring in the short-telomere mouse species M. spretus. Introducing this variation into M. musculus is sufficient to reduce the telomere length set point in the germline and generate mice with human-length telomeres. While these mice are fertile and appear healthy, the regenerative capacity of their colonic epithelium is compromised. The engineered Telomouse reported here demonstrates a dominant role of RTEL1 in telomere length regulation and provides a unique model for aging and cancer.
High-resolution (1 km) Köppen-Geiger maps for 1901–2099 based on constrained CMIP6 projections
Authors: Beck Hylke E. , McVicar Tim R. , Vergopolan Noemi , Berg Alexis , Lutsko Nicholas J. , Dufour Ambroise , Zeng Zhenzhong , Jiang Xin , van Dijk Albert I. J. M. , Miralles Diego G.
Published: October 2023

We introduce Version 2 of our widely used 1-km Köppen-Geiger climate classification maps for historical and future climate conditions. The historical maps (encompassing 1901–1930, 1931–1960, 1961–1990, and 1991–2020) are based on high-resolution, observation-based climatologies, while the future maps (encompassing 2041–2070 and 2071–2099) are based on downscaled and bias-corrected climate projections for seven shared socio-economic pathways (SSPs). We evaluated 67 climate models from the Coupled Model Intercomparison Project phase 6 (CMIP6) and kept a subset of 42 with the most plausible CO2-induced warming rates. We estimate that from 1901–1930 to 1991–2020, approximately 5% of the global land surface (excluding Antarctica) transitioned to a different major Köppen-Geiger class. Furthermore, we project that from 1991–2020 to 2071–2099, 5% of the land surface will transition to a different major class under the low-emissions SSP1-2.6 scenario, 8% under the middle-of-the-road SSP2-4.5 scenario, and 13% under the high-emissions SSP5-8.5 scenario. The Köppen-Geiger maps, along with associated confidence estimates, underlying monthly air temperature and precipitation data, and sensitivity metrics for the CMIP6 models, can be accessed at www.gloh2o.org/koppen.
Fusion localization for indoor airplane inspection using visual inertial odometry and ultrasonic RTLS
Authors: Park Ingyoon , Cho Sangook
Published: October 2023

In this paper, the fusion localization system for the visual inertial odometry (VIO) and ultrasonic real-time localization system (RTLS) for indoor airplane inspection using drones is proposed. In a hangar environment, either trilateration-based RTLS or vision-based localization shows disadvantages and neither can be used alone. In this research, we design a configuration of VIO suitable for hangar environment and outlier filter on ultrasonic RTLS for non-line of sight situations, so that both can be fused using graph optimization. The proposed solution can provide more accurate localization than the visual odometry-only system as well as continue estimating positions in the absence of RTLS data. Localization and real-time performance of the proposed algorithm are evaluated through experimentation in a hangar and a flight test in an outdoor space.
Attenuates reactive oxygen species: induced pyroptosis via activation of the Nrf2/HO-1 signal pathway in models of trigeminal neuralgia
Authors: Liu Mingxing , Wang Yongyi , Li Shengli , Hou Xiaoqun , Li Tong , Xu Zhiming , Chen Feng , Zhou Yong , Xia Lei , Wang Weimin
Published: October 2023

In this study, we examined the impact of demyelinating and neuroinflammation on trigeminal neuralgia (TN) by utilizing models of chronic constriction injury to the infraorbital nerve (CCI). The CCI rats were treated with either VX-765 (an inhibitor of caspase-1) or a control solution of PBS/DMSO to observe the effects on neurobehavioral and neuropathological outcomes. The histochemical changes, pyroptosis-related proteins were assessed using immunohistochemistry, Elisa, and western blotting. RSC96 cells were pretreated with belnacasan (VX-765, an inhibitor of caspase-1), Gasdermin D(GSDMD)-targeting siRNAs, cobalt protoporphyrin (CoPP) or zinc protoporphyrin (Znpp) before being exposed to H2O2. Following these treatments, the Reactive oxygen species (ROS) level, cell viability, percentage of pyroptosis, pyroptosis-related proteins, nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1 level was measured. The scanning electron microscopy revealed increased ball-like bulge and membrane pore formation in the CCI group. In the CCI and CCI+ Vehicle groups, we found ROS level and expression of pyroptosis-related proteins increased. While, treatment with VX-765resulted in a decreased expression of GSDMD, IL-1, IL-18, and caspase-1 decreased. In the in-vitro study, RSC96 cells showed mild pyroptosis and overall mild edema after being exposed to H2O2. The ROS level, percentage of pyroptosis, pyroptosis-related proteins, Nrf2 and HO-1 level increased significantly in the H2O2 group. While, the percentage of pyroptosis and pyroptosis-related proteins decreased significantly in the H2O2 + VX-765 group, H2O2 + siRNA group, and H2O2 + VX-765 + siRNA group. After treatment with HO-1-inhibitor Znpp and HO-1-activator Copp, the percentage of pyroptosis and pyroptosis-related proteins increased and decreased significantly respectively. In conclusions, the pyroptosis of Schwann cell in the CCI model generated the demyelination of TN nerve. The ROS is an upstream event of NLRP3 inflammasome activation which induced eventual pyroptosis. The Nrf2/HO-1 signaling pathway could protect the H2O2-induced pyroptosis in RSC96 cells.
Interactive effects of two rodent species on the seed dispersal of Japanese walnut
Authors: Okawa Ryunosuke , Saitoh Takashi , Noda Takashi
Published: October 2023

The effects of seed dispersers on plant fitness (seed dispersal effectiveness, SDE) have been evaluated based on the number (quantity) and recruitment probability (quality) of dispersed seeds. Although seeds of most zoochorous species are dispersed by two or more animal species, which may interact with each other, SDE has often been studied assuming a one-plant and one-animal species system. We compared the SDE of Japanese walnut (Juglans ailanthifolia) between squirrel-only and squirrel-mouse sites in natural forests of Hokkaido, Japan, and found that the SDE from the red squirrel (Sciurus vulgaris), considered a primary seed disperser, was altered by an alternative seed disperser species, the Japanese wood mouse (Apodemus speciosus). Seed removal rates at the squirrel-mouse site were significantly higher than those at the squirrel-only site, and both dispersed seeds and seedlings were less aggregated, with a strongly repulsive relationship with adult conspecific trees at the squirrel-mouse site. Seedlings established themselves at a location with fewer medium-sized trees (< 10 cm DBH) at the squirrel-mouse site. These results suggest that the interactive effect of the rodent species affects the SDE of Japanese walnut.
Boosting wheat yield, profitability and NUE with prilled and nano urea in conservation tillage
Authors: Kumar Nitesh , Tripathi S. C. , Yadav D. B. , Samota Shiv Ram , Venkatesh Karnam , Sareen Sindhu , Singh Gyanendra
Published: October 2023

Rice–wheat production in the Indo-gangetic plains (IGPs) of India faces major concerns such as depleting resources, rice residue burning, excessive fertilizer use, and decreasing nitrogen use efficiency. These issues threaten sustainable crop production in the future. Therefore, a field study was conducted during the winter seasons of 2020–21 and 2021–22 to evaluate the effect of combined conventional and nano fertilizers on nitrogen application just before or after irrigation to improve wheat productivity, profitability and NUE under conservation tillage. The study evaluated eight treatment combinations of nitrogen application through conventionally applied urea (46% N) and foliar applied nano urea (4% N) under zero tillage with rice residue retention. Results revealed that growth, physiological indices, yield, and quality parameters were enhanced with the application of 150 kg N/ha in three equal splits as basal and just before 1st and 2nd irrigation alone (T2) or along with a spray of nano urea (T5) compared to other treatments. T5 recorded 7.2%, 8.5%, and 7.8% more plant dry matter, number of tillers, and grain yield, respectively, over the conventional practice of applying 150 kg N/ha in three equal splits as basal and 7–10 days after 1st and 2nd irrigation (T3, farmers practice). Although, T2 showed similar results to T5, T5 recorded significantly higher gross ($2542/ha) and net returns ($1279/ha) than the other treatments. However, the benefit–cost ratio of T2 and T5 was same (2.01). A significant and positive correlation coefficient between grain yield and physiological parameters such as CCI and NDVI confirmed that increasing the nitrogen dose enhanced the chlorophyll content, greenness, and plant vigor. Based on the results, it can be concluded that applying 150 kg N/ha in three equal splits as basal and just before 1st and 2nd irrigation under conservation agriculture, along with a single spray of nano urea (4% N) at 60–65 days after sowing, can improve growth, yield attributes, wheat yield, and NUE compared to farmers practice (T3) in India.
Reproducibility of APT-weighted CEST-MRI at 3T in healthy brain and tumor across sessions and scanners
Authors: Wu Yulun , Wood Tobias C. , Derks Sophie H. A. E. , Pruis Ilanah J. , van der Voort Sebastian , van Zanten Sophie E. M. Veldhuijzen , Smits Marion , Warnert Esther A. H.
Published: October 2023

Amide proton transfer (APT)-weighted chemical exchange saturation transfer (CEST) imaging is a recent MRI technique making its way into clinical application. In this work, we investigated whether APT-weighted CEST imaging can provide reproducible measurements across scan sessions and scanners. Within-session, between-session and between scanner reproducibility was calculated for 19 healthy volunteers and 7 patients with a brain tumor on two 3T MRI scanners. The APT-weighted CEST effect was evaluated by calculating the Lorentzian Difference (LD), magnetization transfer ratio asymmetry (MTRasym), and relaxation-compensated inverse magnetization transfer ratio (MTRREX) averaged in whole brain white matter (WM), enhancing tumor and necrosis. Within subject coefficient of variation (COV) calculations, Bland–Altman plots and mixed effect modeling were performed to assess the repeatability and reproducibility of averaged values. The group median COVs of LD APT were 0.56% (N = 19), 0.84% (N = 6), 0.80% (N = 9) in WM within-session, between-session and between-scanner respectively. The between-session COV of LD APT in enhancing tumor (N = 6) and necrotic core (N = 3) were 4.57% and 5.67%, respectively. There were no significant differences in within session, between session and between scanner comparisons of the APT effect. The COVs of LD and MTRREX were consistently lower than MTRasym in all experiments, both in healthy tissues and tumor. The repeatability and reproducibility of APT-weighted CEST was clinically acceptable across scan sessions and scanners. Although MTRasym is simple to acquire and compute and sufficient to provide robust measurement, it is beneficial to include LD and MTRREX to obtain higher reproducibility for detecting minor signal difference in different tissue types.
Ageing influences detrusor contractions to prostaglandin, angiotensin, histamine and 5-HT (serotonin), independent to the Rho kinase and extracellular calcium pathways
Authors: Phelps Charlotte , Chess-Williams Russ , Moro Christian
Published: October 2023

Ageing is associated with deteriorating urinary bladder function and an increasing prevalence of disorders such as underactive bladder. There are suggestions that G protein-coupled receptor (GPCR) second messenger pathways are altered during ageing, rather than the receptor proteins themselves. The aim of this study was to identify age-related variations in GPCR activation systems in urinary bladder smooth muscle (detrusor). Isolated porcine detrusor strips were mounted in organ baths and contractile responses induced by receptor agonists were assessed and compared between juvenile (6 months) and adult (2 years) animals. The effects of drugs disrupting intracellular calcium signalling were also studied. Adult tissue was far more sensitive to stimulation by 5-hydroxytryptamine (42% greater increase than juvenile), prostaglandin-E2 (26% greater increase), and angiotensin-II (39% greater increase), however less sensitive to histamine. Although nifedipine and Y-27632 impacted the contraction to all agonists, there were no significant differences between juvenile and adult detrusor. Impairment of IP3-mediated calcium release by 2-aminoethyl diphenylborinate had no effect on any contractile activity, except for neurokinin-A which inhibited both juvenile and adult detrusor, and prostaglandin-E2 which inhibited juvenile. Carbachol, histamine, 5-hydroxytryptamine, and angiotensin-II were not affected by the application of 2-aminoethyl diphenylborinate. In conclusion, the contractile responses to all the GPCR agonists involved extracellular calcium influx and calcium sensitisation, but for prostaglandin-E2 the dependence on calcium from intracellular sources was greater in the younger animals.
Mutual-information based optimal experimental design for hyperpolarized $$^{13}$$C-pyruvate MRI
Authors: Jha Prashant K. , Walker Christopher , Mitchell Drew , Oden J. Tinsley , Schellingerhout Dawid , Bankson James A. , Fuentes David T.
Published: October 2023

A key parameter of interest recovered from hyperpolarized (HP) MRI measurements is the apparent pyruvate-to-lactate exchange rate, $$k_{{\textrm PL}}$$

k

P
L

, for measuring tumor metabolism. This manuscript presents an information-theory-based optimal experimental design approach that minimizes the uncertainty in the rate parameter, $$k_{{\textrm PL}}$$

k

P
L

, recovered from HP-MRI measurements. Mutual information is employed to measure the information content of the HP measurements with respect to the first-order exchange kinetics of the pyruvate conversion to lactate. Flip angles of the pulse sequence acquisition are optimized with respect to the mutual information. A time-varying flip angle scheme leads to a higher parameter optimization that can further improve the quantitative value of mutual information over a constant flip angle scheme. However, the constant flip angle scheme, 35 and 28 degrees for pyruvate and lactate measurements, leads to an accuracy and precision comparable to the variable flip angle schemes obtained from our method. Combining the comparable performance and practical implementation, optimized pyruvate and lactate flip angles of 35 and 28 degrees, respectively, are recommended.
Identification of anoikis-related subtypes and immune landscape in kidney renal clear cell carcinoma
Authors: Ding Wencong , Zhang Min , Zhang Ping , Zhang Xianghong , Sun Junwei , Lin Biying
Published: October 2023

Anoikis is a specific form of programmed cell death induced by the loss of cell contact with the extracellular matrix and other cells, and plays an important role in organism development, tissue homeostasis, disease development and tumor metastasis. We comprehensively investigated the expression patterns of anoikis-related genes (ARGs) in kidney renal clear cell carcinoma (KIRC) from public databases. Anoikis-related prognostic signatures were established based on four ARGs expression, in which KIRC patients were assigned different risk scores and divided into two different risk groups. In addition, four ARGs expression was validated by qRT-PCR. A better prognosis was observed in the low-risk group, but with lower immune activity (including immune cells and immune-related functions) in the tumor microenvironment. Combined with the relevant clinical characteristics, a nomogram for clinical application was established. Receiver operating characteristics (ROC) and calibration curves were constructed to demonstrate the predictive power of this risk signature. In addition, higher risk scores were significantly and positively correlated with higher gene expression of tumor mutation load (TMB), immune checkpoints (ICPs) and mismatch repair (MMR)-related proteins in general. The results also suggested that the high-risk group was more sensitive to immunotherapy and certain chemotherapeutic agents. Anoikis-related prognostic signatures may provide a better understanding of the roles of ARGs and offer new perspectives for clinical prognosis and individualized treatment.
Knockdown of lncRNA MALAT1 attenuates renal interstitial fibrosis through miR-124-3p/ITGB1 axis
Authors: Xia Weiping , Chen Xiang , Zhu Zewu , Chen Hequn , Li Bingsheng , Wang Kangning , Huang Li , Liu Zhi , Chen Zhi
Published: October 2023

Renal interstitial fibrosis (RIF) considered the primary irreversible cause of chronic kidney disease. Recently, accumulating studies demonstrated that lncRNAs play an important role in the pathogenesis of RIF. However, the underlying exact mechanism of lncRNA MALAT1 in RIF remains barely known. Here, the aim of our study was to investigate the dysregulate expression of lncRNA MALAT1 in TGF-β1 treated HK2/NRK-49F cells and unilateral ureteral obstruction (UUO) mice model, defining its effects on HK2/NRK-49F cells and UUO mice fibrosis process through the miR-124-3p/ITGB1 signaling axis. It was found that lncRNA MALAT1 and ITGB1 was significantly overexpression, while miR-124-3p was downregulated in HK2/NRK-49F cells induced by TGF-β1 and in UUO mice model. Moreover, knockdown of lncRNA MALAT1 remarkably downregulated the proteins level of fibrosis-related markers, ITGB1, and upregulated the expression of epithelial marker E-cadherin. Consistently, mechanistic studies showed that miR-124-3p can directly binds to lncRNA MALAT1 and ITGB1. And the protect effect of Len-sh-MALAT1 on fibrosis related protein levels could be partially reversed by co-transfected with inhibitor-miR-124-3p. Moreover, the expression trend of LncRNA MALAT1/miR-124-3p/ITGB1 in renal tissues of patients with obstructive nephropathy (ON) was consistent with the results of cell and animal experiments. Taken together, these results indicated that lncRNA MALAT1 could promote RIF process in vitro and in vivo via the miR-124-3p/ITGB1 signaling pathway. These findings suggest a new regulatory pathway involving lncRNA MALAT1, which probably serves as a potential therapeutic target for RIF.
Genome-wide identification and comprehensive analysis heat shock transcription factor (Hsf) members in asparagus (Asparagus officinalis) at the seeding stage under abiotic stresses
Authors: Li Caihua , Li Yuhuan , Zhou Zeng , Huang Yudi , Tu Zunzun , Zhuo Xin , Tian Dingyuan , Liu Yibo , Di Hongli , Lin Ze , Shi Mingxin , He Xue , Xu Haiyu , Zheng Yi , Mu Zhongsheng
Published: October 2023

Heat shock transcription factors (Hsf) are pivotal as essential transcription factors. They function as direct transcriptional activators of genes regulated by thermal stress and are closely associated with various abiotic stresses. Asparagus (Asparagus officinalis) is a vegetable of considerable economic and nutritional significance, abundant in essential vitamins, minerals, and dietary fiber. Nevertheless, asparagus is sensitive to environmental stresses, and specific abiotic stresses harm its yield and quality. In this context, Hsf members have been discerned through the reference genome, and a comprehensive analysis encompassing physical and chemical attributes, evolutionary aspects, motifs, gene structure, cis-acting elements, collinearity, and expression patterns under abiotic stresses has been conducted. The findings identified 18 members, categorized into five distinct subgroups. Members within each subgroup exhibited analogous motifs, gene structures, and cis-acting elements. Collinearity analysis unveiled a noteworthy pattern, revealing that Hsf members within asparagus shared one, two, and three pairs with counterparts in Arabidopsis, Oryza sativa, and Glycine max, respectively.Furthermore, members displayed tissue-specific expression during the seedling stage, with roots emerging as viable target tissue. Notably, the expression levels of certain members underwent modification under the influence of abiotic stresses. This study establishes a foundational framework for understanding Hsf members and offers valuable insights into the potential application of molecular breeding in the context of asparagus cultivation.