bluuebunny/crossref_metadata_2025 huggingface dataset:
Membrane Fouling Behavior of Forward Osmosis for Fruit Juice Concentration
Authors: Li Zihe , Wu Chongde , Huang Jun , Zhou Rongqing , Jin Yao
Published: August 2021

Forward osmosis (FO) technology has a broad application prospect in the field of liquid food concentration because of the complete retention of flavor components and bioactive substances. Membrane fouling is the main obstacle affecting the FO performance and concentration efficiency. This work systematically investigated the membrane fouling behavior of the FO process for fruit juice concentration elucidated by the models of resistance-in-series, xDLVO theory and FTIR analysis. The results show that the AL-FS mode was more suitable for concentrating orange juice. Increasing the cross-flow rate and pretreatment of feed solutions can effectively improve the water flux and reduce the fouling resistance. The ATR-FTIR analysis revealed that the fouling layer of orange juice was mainly composed of proteins and polysaccharides, and the pretreatment of microfiltration can greatly reduce the content of the major foulant. There was an attractive interaction between the FO membrane and orange juice foulants; by eliminating those foulants, the microfiltration pretreatment then weakened such an attractive interaction and effectively prevented the fouling layer from growing, leading to a lower process resistance and, finally, resulting in a great improvement of concentration efficiency.
Structural Arrangement Produced by Concanavalin A Binding to Homomeric GluK2 Receptors
Authors: Gonzalez Cuauhtemoc U. , Carrillo Elisa , Berka Vladimir , Jayaraman Vasanthi
Published: August 2021

Kainate receptors are members of the ionotropic glutamate receptor family. They form cation-specific transmembrane channels upon binding glutamate that desensitize in the continued presence of agonists. Concanavalin A (Con-A), a lectin, stabilizes the active open-channel state of the kainate receptor and reduces the extent of desensitization. In this study, we used single-molecule fluorescence resonance energy transfer (smFRET) to investigate the conformational changes underlying kainate receptor modulation by Con-A. These studies showed that Con-A binding to GluK2 homomeric kainate receptors resulted in closer proximity of the subunits at the dimer–dimer interface at the amino-terminal domain as well as between the subunits at the dimer interface at the agonist-binding domain. Additionally, the modulation of receptor functions by monovalent ions, which bind to the dimer interface at the agonist-binding domain, was not observed in the presence of Con-A. Based on these results, we conclude that Con-A modulation of kainate receptor function is mediated by a shift in the conformation of the kainate receptor toward a tightly packed extracellular domain.
Development of Environment-Friendly Membrane for Oily Industrial Wastewater Filtration
Authors: Alquraish Mohammed , Jeng Yong Tzyy , Kchaou Mohamed , Munusamy Yamuna , Abuhasel Khaled
Published: August 2021

Latex phase blending and crosslinking method was used in this research work to produce nitrile butadiene rubber-graphene oxide (NBR-GO) membranes. This fabrication technique is new and yields environmentally friendly membranes for oil-water separation. GO loading was varied from 0.5 to 2.0 part per hundred-part rubber (pphr) to study its effect on the performance of NBR-GO membrane. GO was found to alter the surface morphology of the NBR matrix by introducing creases and fold on its surface, which then increases the permeation flux and rejection rate efficiency of the membrane. X-Ray diffraction analysis proves that GO was well dispersed in the membrane due to the non-existence of GO fingerprint diffraction peak at 2θ value of 10–12° in the membrane samples. The membrane filled with 2.0 pphr GO has the capability to permeate 7688.54 Lm−2 h−1 water at operating pressure of 0.3 bar with the corresponding rejection rate of oil recorded at 94.89%. As the GO loading increases from 0.5 to 2.0 pphr, fouling on the membrane surface also increases from Rt value of 45.03% to 87.96%. However, 100% recovery on membrane performance could be achieved by chemical backwashing.
Structure and Multistage Martensite Transformation in Nanocrystalline Ti-50.9Ni Alloy
Authors: Poletika Tamara M. , Girsova Svetlana L. , Lotkov Aleksander I. , Kudryachov Andrej N. , Girsova Natalya V.
Published: August 2021

An electron microscopic study of the evolution of the size, morphology, and spatial distribution of coherent Ti3Ni4 particles with a change in the aging temperature in a nanocrystalline (NC) Ti-50.9 at % Ni alloy with an inhomogeneous grain–subgrain B2-austenitic nanostructure has been carried out. It was found that with an increase in the aging temperature, along with a change in the size and shape of Ti3Ni4 nanoparticles, their spatial distribution changes from location at dislocations to precipitates at subboundaries. Research has shown that the presence of different types of internal interfaces in the nanostructure contributes to the heterogeneous distribution of coherent Ti3Ni4 nanoparticles in the volume of the B2 matrix, which is associated with the precipitation of particles in the region of low-angle subboundaries and the suppression of the Ti3Ni4 precipitation in nanograins with high-angle boundaries. The difference in the structural-phase state of nanograins and subgrains regions is the main reason for the implementation of the anomalous R-phase transformation effect in the sequence of multistage martensitic transformations B2↔R↔B19′.
Hot Deformation Behavior of a Co-Spray-Formed Al-Si/7075 Bimetallic Gradient Alloy
Authors: Yu Lei , Jiang Sida , Cao Fuyang , Shen Hongxian , Zhang Lunyong , Gu Xu , Song Heqian , Sun Jianfei
Published: August 2021

In this paper, we report the results obtained from the hot compression behaviour of co-spray-formed Al-Si/7075 bimetallic gradient alloys by investigating the five selected layers divided evenly from top to bottom. It was found that with a decrease in the strain rate and an increase in temperature, the hot compression flow stress of deposited alloys decreases gradually, as expected. The relationship between flow stress, temperature and strain rate follows the Arrhenius relationship. The deformation activation energy of the co-spray formed gradient alloy gradually increases from bottom to top in the height direction, which are 100.15, 167.35, 185.54, 208.78 and 220.83 KJ/mol, indicating that the degree of difficulty in the deformation of the alloy is affected by changes in the silicon content of the alloy. Finally, the flow stress constitutive equation is calculated for each layer of alloy material, which is instructive for the subsequent study of the densification process of co-spray-formed gradient alloys.
A Plant Endophytic Bacterium, Burkholderia seminalis Strain 869T2, Promotes Plant Growth in Arabidopsis, Pak Choi, Chinese Amaranth, Lettuces, and Other Vegetables
Authors: Hwang Hau-Hsuan , Chien Pei-Ru , Huang Fan-Chen , Hung Shih-Hsun , Kuo Chih-Horng , Deng Wen-Ling , Chiang En-Pei Isabel , Huang Chieh-Chen
Published: August 2021

Plant endophytic bacteria live inside host plants, can be isolated from surface-sterilized plant tissues, and are non-pathogenic. These bacteria can assist host plants in obtaining more nutrients and can improve plant growth via multiple mechanisms. Certain Gram-negative Burkholderia species, including rhizobacteria, bioremediators, and biocontrol strains, have been recognized for their plant-growth-promoting abilities, while other isolates have been identified as opportunistic plant or human pathogens. In this study, we observed the auxin production, siderophore synthesis, and phosphate solubilization abilities of B. seminalis strain 869T2. Our results demonstrated that strain 869T2 promoted growth in Arabidopsis, ching chiang pak choi, pak choi, loose-leaf lettuce, romaine lettuce, red leaf lettuce, and Chinese amaranth. Leafy vegetables inoculated with strain 869T2 were larger, heavier, and had more and larger leaves and longer and heavier roots than mock-inoculated plants. Furthermore, inoculations of strain 869T2 into hot pepper caused increased flower and fruit production, and a higher percentage of fruits turned red. Inoculation of strain 869T2 into okra plants resulted in earlier flowering and increased fruit weight. In conclusion, the plant endophytic bacterium Burkholderia seminalis 869T2 exerted positive effects on growth and production in several plant species.
Adaptations for Pressure and Temperature in Dihydrofolate Reductases
Authors: Penhallurick Ryan W. , Durnal Maya D. , Harold Alliyah , Ichiye Toshiko
Published: August 2021

Enzymes from extremophilic microbes that live in extreme conditions are generally adapted so that they function under those conditions, although adaptations for extreme temperatures and pressures can be difficult to unravel. Previous studies have shown mutation of Asp27 in Escherichia coli dihydrofolate reductase (DHFR) to Glu27 in Moritella profunda (Mp). DHFR enhances activity at higher pressures, although this may be an adaptation for cold. Interestingly, MpDHFR unfolds at ~70 MPa, while Moritella yayanosii (My) was isolated at depths corresponding to ~110 MPa, indicating that MyDHFR might be adapted for higher pressures. Here, these adaptations are examined using molecular dynamics simulations of DHFR from different microbes in the context of not only experimental studies of activity and stability of the protein but also the evolutionary history of the microbe. Results suggest Tyr103 of MyDHFR may be an adaptation for high pressure since Cys103 in helix F of MpDHFR forms an intra-helix hydrogen bond with Ile99 while Tyr103 in helix F of MyDHFR forms a hydrogen bond with Leu78 in helix E. This suggests the hydrogen bond between helices F and E in MyDHFR might prevent distortion at higher pressures.
Experimental Uncertainty Analysis for the Particle Size Distribution for Better Understanding of Batch Grinding Process
Authors: Delgado José , Lucay Freddy A. , Sepúlveda Felipe D.
Published: August 2021

Uncertainty in industrial processes is very common, but it is particularly high in the grinding process (GP), due to the set of interacting operating/design parameters. This uncertainty can be evaluated in different ways, but, without a doubt, one of the most important parameters that characterise all GPs is the particle size distribution (PSD). However, is the PSD a good way to quantify the uncertainty in the milling process? This is the question we attempt to answer in this paper. To do so, we use 10 experimental grinding repetitions, 3 grinding times, and 14 Tyler meshes (more than 400 experimental results). The most relevant results were compared for the weight percentage for each size (WPES), cumulative weight undersize (CWU), or the use of particle size distribution models (PSDM), in terms of continuous changes in statistical parameters in WPES for different grinding times. The probability distribution was found to be changeable when reporting the results of WPES/CWU/PSDM, we detected the over-/under-estimation of uncertainty when using WPES/CWU, and variations in the relationships between sizes were observed when using WPES/CWU. Finally, our conclusion was that the way in which the data are analysed is not trivial, due to the possible deviations that may occur in the uncertainty process.
A Review of Flotation Physical Froth Flow Modifiers
Authors: Jera Tawona M. , Bhondayi Clayton
Published: August 2021

Over the past few decades, the need to process more minerals while lowering capital costs has led to an increase in the size of flotation cells, e.g., 0.03 m3 to 1000 m3. However, this increase has created new challenges in the operation and design of industrial flotation cells, particularly in terms of froth removal, because the distance the froth must travel increases with an increase in the flotation cell diameter. This has a negative impact on recovery. Physical froth flow modifiers can be used to improve froth removal. Their major functions are to modify and optimise the flow of the froth, improve froth drainage, reduce dead zones, and improve froth flow and removal dynamics. Therefore, physical froth flow modifiers are discussed, evaluated, and compared in this paper. The literature indicates that physical froth flow modifiers such as crowders and launders are used extensively as industrial solutions to enhance froth transport and recovery in large flotation cells. Other modifiers (including froth baffles and froth scrapers) have been found to have a profound effect on local froth phase sub-processes, including drainage and bubble coalescence. However, industrial uptake is either dwindling or limited to small-volume rectangular/U-shaped cells in the case of scrapers, or, there is no uptake at all in the case of froth baffles. Further research on how some of the physical modifiers (e.g., baffles and launders) impact the selectivity of particles is required.
A Critical Evaluation and Modification of the Padé–Laplace Method for Deconvolution of Viscoelastic Spectra
Authors: Shams Es-haghi Siamak , Gardner Douglas J.
Published: August 2021

This paper shows that using the Padé–Laplace (PL) method for deconvolution of multi-exponential functions (stress relaxation of polymers) can produce ill-conditioned systems of equations. Analysis of different sets of generated data points from known multi-exponential functions indicates that by increasing the level of Padé approximants, the condition number of a matrix whose entries are coefficients of a Taylor series in the Laplace space grows rapidly. When higher levels of Padé approximants need to be computed to achieve stable modes for separation of exponentials, the problem of generating matrices with large condition numbers becomes more pronounced. The analysis in this paper discusses the origin of ill-posedness of the PL method and it was shown that ill-posedness may be regularized by reconstructing the system of equations and using singular value decomposition (SVD) for computation of the Padé table. Moreover, it is shown that after regularization, the PL method can deconvolute the exponential decays even when the input parameter of the method is out of its optimal range.
Effect of Ellagic Acid on Seizure Threshold in Two Acute Seizure Tests in Mice
Authors: Pieróg Mateusz , Socała Katarzyna , Wyska Elżbieta , Poleszak Ewa , Wlaź Piotr
Published: August 2021

Ellagic acid (EA) is a natural dietary polyphenol that has many beneficial properties, including anti-inflammatory, antioxidant, antiviral, antibacterial, and neuroprotective effects. Studies have revealed that EA may modulate seizure activity in chemically induced animal models of seizures. Therefore, the aim of the present study was to investigate the effect of EA on the seizure threshold in two acute seizure tests in male mice, i.e., in the intravenous (i.v.) pentylenetetrazole (PTZ) seizure test and in the maximal electroshock seizure threshold (MEST) test. The obtained results showed that EA (100 mg/kg) significantly elevated the threshold for both the first myoclonic twitch and generalized clonic seizure in the i.v. PTZ seizure test. At the highest dose tested (200 mg/kg), EA increased the threshold for tonic hindlimb extension in the MEST test. EA did not produce any significant changes in motor coordination (assessed in the chimney test) or muscular strength (investigated in the grip-strength test). The plasma and total brain concentration-time profiles of EA after intraperitoneal and oral administration were also determined. Although further studies are necessary to confirm the anticonvulsant activity of EA, our findings suggest that it may modulate seizure susceptibility in animal models.
Modification of Hemodialysis Membranes for Efficient Circulating Tumor Cell Capture for Cancer Therapy
Authors: Jarvas Gabor , Szerenyi Dora , Tovari Jozsef , Takacs Laszlo , Guttman Andras
Published: August 2021

Background: It is well known that more than 90% of cancer deaths are due to metastases. However, the entire tumorigenesis process is not fully understood, and it is evident that cells spreading from the primary tumor play a key role in initiating the metastatic process. Tumor proliferation and invasion also elevate the concentration of regular and irregular metabolites in the serum, which may alter the normal function of the entire human homeostasis and possibly causes cancer metabolism syndrome, also referred to as cachexia. Methods: We report on the modification of commercially available hemodialysis membranes to selectively capture circulating tumor cells from the blood stream by means of immobilized human anti-EpCAM antibodies on the inner surface of the fibers. All critical steps are described that required in situ addition of the immuno-affinity feature to hemodialyzer cartridges in order to capture EpCAM positive circulating tumor cells, which represents ~80% of cancer cell types. Results: The cell capture efficiency of the suggested technology was demonstrated by spiking HCT116 cancer cells both into buffer solution and whole blood and run through on the modified cartridge. Flow cytometry was used to quantitatively evaluate the cell clearance performance of the approach. Conclusions: The suggested modification has no significant effect on the porous structure of the hemodialysis membranes; it keeps its cytokine removal capability, addressing cachexia simultaneously with CTC removal.
Structural Characterization of a Macrocyclic Peptide Modulator of the PD-1/PD-L1 Immune Checkpoint Axis
Authors: Zyla Edyta , Musielak Bogdan , Holak Tad A. , Dubin Grzegorz
Published: August 2021

The clinical success of PD-1/PD-L1 immune checkpoint targeting antibodies in cancer is followed by efforts to develop small molecule inhibitors with better penetration into solid tumors and more favorable pharmacokinetics. Here we report the crystal structure of a macrocyclic peptide inhibitor (peptide 104) in complex with PD-L1. Our structure shows no indication of an unusual bifurcated binding mode demonstrated earlier for another peptide of the same family (peptide 101). The binding mode relies on extensive hydrophobic interactions at the center of the binding surface and an electrostatic patch at the side. An interesting sulfur/π interaction supports the macrocycle-receptor binding. Overall, our results allow a better understanding of forces guiding macrocycle affinity for PD-L1, providing a rationale for future structure-based inhibitor design and rational optimization.
Molecular Details on Multiple Cofactor Containing Redox Metalloproteins Revealed by Infrared and Resonance Raman Spectroscopies
Authors: Silveira Célia M. , Zuccarello Lidia , Barbosa Catarina , Caserta Giorgio , Zebger Ingo , Hildebrandt Peter , Todorovic Smilja
Published: August 2021

Vibrational spectroscopy and in particular, resonance Raman (RR) spectroscopy, can provide molecular details on metalloproteins containing multiple cofactors, which are often challenging for other spectroscopies. Due to distinct spectroscopic fingerprints, RR spectroscopy has a unique capacity to monitor simultaneously and independently different metal cofactors that can have particular roles in metalloproteins. These include e.g., (i) different types of hemes, for instance hemes c, a and a3 in caa3-type oxygen reductases, (ii) distinct spin populations, such as electron transfer (ET) low-spin (LS) and catalytic high-spin (HS) hemes in nitrite reductases, (iii) different types of Fe-S clusters, such as 3Fe-4S and 4Fe-4S centers in di-cluster ferredoxins, and (iv) bi-metallic center and ET Fe-S clusters in hydrogenases. IR spectroscopy can provide unmatched molecular details on specific enzymes like hydrogenases that possess catalytic centers coordinated by CO and CN− ligands, which exhibit spectrally well separated IR bands. This article reviews the work on metalloproteins for which vibrational spectroscopy has ensured advances in understanding structural and mechanistic properties, including multiple heme-containing proteins, such as nitrite reductases that house a notable total of 28 hemes in a functional unit, respiratory chain complexes, and hydrogenases that carry out the most fundamental functions in cells.
Special Issue: “Peter Biely, A Pioneering Researcher in the Enzymology of Plant Biomass Degradation”
Authors: Hrmova Maria
Published: August 2021

As it has been outlined on the website of the Special Issue entitled “Peter Biely, a pioneering researcher in the enzymology of plant biomass degradation” in the journal Molecules (section Macromolecular Chemistry, ISSN 1420-3049), plant biomass is a key renewable resource [...]
Screen-Printed Flexible Thermoelectric Device Based on Hybrid Silver Selenide/PVP Composite Films
Authors: Liu Dan , Zhao Yaxin , Yan Zhuqing , Zhang Zhidong , Zhang Yanjun , Shi Peng , Xue Chenyang
Published: August 2021

In recent years, the preparation of flexible thermoelectric generators by screen printing has attracted wide attention due to easy processing and high-volume production. In this work, we propose an n-type Ag2Se/polymer polyvinylpyrrolidone (PVP) film based on screen printing and investigate the effect of PVP on thermoelectric performance by varying the ratio of PVP. When the content ratio of Ag2Se to PVP is 30:1, i.e., PI30, the fabricated PI30 film has the best thermoelectric property. The maximum power factor (PF) of the PI30 is 4.3 μW·m−1·K−2, and conductivity reaches 81% of its initial value at 1500 bending cycles. Then, the film thermoelectric generator (F-TEG) fabricated by PI30 is tested for practical application; the output voltage and the maximum output power are 21.6 mV and 233.3 nW at the temperature difference of 40 K, respectively. This work demonstrates that the use of PVP combined with screen printing to prepare F-TEG is a simple and rapid method, which provides an efficient preparation solution for the development of environmentally friendly and wearable flexible thermoelectric devices.
Numerical Investigation of Graphene and STO Based Tunable Terahertz Absorber with Switchable Bifunctionality of Broadband and Narrowband Absorption
Authors: Liu Yan , Huang Rui , Ouyang Zhengbiao
Published: August 2021

A graphene metamaterial and strontium titanate (STO)-based terahertz absorber with tunable and switchable bifunctionality has been numerically investigated in this work. Through electrically tuning the Fermi energy level of the cross-shaped graphene, the bandwidth of the proposed absorber varies continuously from 0.12 THz to 0.38 THz with the absorptance exceeding 90%, which indicates the functionality of broadband absorption. When the Fermi energy level of the cross-shaped graphene is 0 eV, the proposed absorber exhibits the other functionality of narrowband absorption owing to the thermal control of the relative permittivity of STO, and the rate of change of the center frequency is 50% ranging from 0.56 THz to 0.84 THz. The peak intensity of the narrowband absorption approximates to nearly 100% through adjusting the Fermi energy level of the graphene strips. The calculated results indicate that it is not sensitive to the polarization for wide incidence angles. The proposed absorber can realize tunable bifunctionality of broadband absorption with a tunable bandwidth and narrowband absorption with a tunable center frequency, which provides an alternative design opinion of the tunable terahertz devices with high performance for high-density integrated systems.
Themes in Train-the-Trainer Nutrition Education Interventions Targeting Middle School Students: A Systematic Review
Authors: St. Pierre Christine , Guan Win , Barry Leah , Dease Grace , Gottlieb Sydney , Morris Arielle , Merrill Jamison , Sacheck Jennifer M.
Published: August 2021

Context-appropriate nutrition education interventions targeting middle school students have the potential to promote healthy dietary patters that may help prevent unnecessary weight gain at a point in childhood development when youth experience increasing agency over their food choices. The aim of this review was to identify and synthesize themes in train-the-trainer approaches, intervention content and delivery, and youth receptivity across teacher, mentor, and peer-led nutrition education interventions that targeted middle school-age youth in urban, primarily low-income settings. A systematic, electronic literature search was conducted in seven electronic databases, PubMed/Medline, CINAHL, ERIC, PsycINFO, Scopus, SPORTDiscus, and Cochrane CENTRAL, using fixed inclusion and exclusion criteria. A total of 53 papers representing 39 unique interventions were selected for data extraction and quality assessment. A framework synthesis approach was used to organize the interventions into six categories and identify themes according to whether the intervention was classroom-based or out-of-school-based and whether adults, cross-age peers or same-age peers delivered the intervention. Ten of the interventions contained multiple components such that they were included in two of the categories. The review findings indicated that trainings should be interactive, include opportunities to role-play intervention scenarios and provide follow-up support throughout intervention delivery. Interventions targeting middle school youth should include positive messaging and empower youth to make healthy choices within their specific food environment context.
International Latin American Survey on Pediatric Intestinal Failure Team
Authors: Spolidoro José Vicente N. , Souza Mirella C. , Goldani Helena A. S. , Tanzi María N. , Busoni Veronica B. , Padilla Maria del Carmen , Ramirez Nelson E. , Cofre Colomba , Valdivieso Lidia P. , Saure Carola , Jimenez-Arguedas Gabriela , Mateus Mikaelle S. M. , Serra Roberta , Cuadros-Mendonza Carlos , Rivera-Medina Juan , Gattini Daniela , dos Santos Beatriz J. , Plata Clara , Sandy Natascha Silva
Published: August 2021

There is little data on the experience of managing pediatric Intestinal Failure (IF) in Latin America. This study aimed to identify and describe the current organization and practices of the IF teams in Latin America and the Caribbean. An online survey was sent to inquire about the existence of IF teams that managed children on home parenteral nutrition (HPN). Our questionnaire was based on a previously published European study with a similar goal. Twenty-four centers with pediatric IF teams in eight countries completed the survey, representing a total number of 316 children on HPN. The median number of children on parenteral nutrition (PN) at home per team was 5.5 (range 1–50). Teams consisted of the following members: pediatric gastroenterologist and a pediatric surgeon in all teams, dietician (95.8%), nurse (91.7%), social worker (79.2%), pharmacist (70.8%), oral therapist (62.5%), psychologist (58.3%), and physiotherapist (45.8%). The majority of the centers followed international standards of care on vascular access, parenteral and enteral nutrition, and IF medical and surgical management, but a significant percentage reported inability to monitor micronutrients, like vitamins A (37.5%), E (41.7%), B1 (66.7%), B2 (62.5%), B6 (62.5%), active B12 (58.3%); and trace elements—including zinc (29.2%), aluminum (75%), copper (37.5%), chromium (58.3%), selenium (58.3%), and manganese (58.3%). Conclusion: There is wide variation in how IF teams are structured in Latin America—while many countries have well-established Intestinal rehabilitation programs, a few do not follow international standards. Many countries did not report having an IF team managing pediatric patients on HPN.
Risk of Malnutrition upon Admission and after Discharge in Acutely Admitted Older Medical Patients: A Prospective Observational Study
Authors: Andersen Aino Leegaard , Nielsen Rikke Lundsgaard , Houlind Morten Baltzer , Tavenier Juliette , Rasmussen Line J. H. , Jørgensen Lillian Mørch , Treldal Charlotte , Beck Anne Marie , Pedersen Mette Merete , Andersen Ove , Petersen Janne
Published: August 2021

There is a lack of knowledge about malnutrition and risk of malnutrition upon admission and after discharge in older medical patients. This study aimed to describe prevalence, risk factors, and screening tools for malnutrition in older medical patients. In a prospective observational study, malnutrition was evaluated in 128 older medical patients (≥65 years) using the Nutritional Risk Screening 2002 (NRS-2002), the Mini Nutritional Assessment-Short Form (MNA-SF) and the Eating Validation Scheme (EVS). The European Society of Clinical Nutrition (ESPEN) diagnostic criteria from 2015 were applied for diagnosis. Agreement between the screening tools was evaluated by kappa statistics. Risk factors for malnutrition included polypharmacy, dysphagia, depression, low functional capacity, eating-related problems and lowered cognitive function. Malnutrition or risk of malnutrition were prevalent at baseline (59–98%) and follow-up (30–88%). The baseline, follow-up and transitional agreements ranged from slight to moderate. NRS-2002 and MNA-SF yielded the highest agreement (kappa: 0.31 (95% Confidence Interval (CI) 0.18–0.44) to 0.57 (95%CI 0.42–0.72)). Prevalence of risk factors ranged from 17–68%. Applying ESPEN 2015 diagnostic criteria, 15% had malnutrition at baseline and 13% at follow-up. In conclusion, malnutrition, risk of malnutrition and risk factors hereof are prevalent in older medical patients. MNA-SF and NRS-2002 showed the highest agreement at baseline, follow-up, and transitionally.
Stress and Coping Strategies among Nursing Students in Clinical Practice during COVID-19
Authors: Hamadi Hanadi Y , Zakari Nazik M. A. , Jibreel Ebtesam , AL Nami Faisal N. , Smida Jamel A. S. , Ben Haddad Hedi H.
Published: August 2021

Stress is common among nursing students and it has been exacerbated during the COVID-19 pandemic. This study examined nursing students’ stress levels and their coping strategies in clinical practice before and during the COVID-19 pandemic. A repeated-measures study design was used to examine the relationship between nursing students’ stress levels and coping strategies before and during the pandemic. Confirmatory factor analyses were conducted to validate the survey and a student T-test was used to compare the level of stress and coping strategies among 131 nursing students. The STROBE checklist was used. During COVID-19, there was a reliable and accurate relationship between stress and coping strategies. Furthermore, both stress and coping strategy scores were lower before COVID-19 and higher during COVID-19. Nursing students are struggling to achieve a healthy stress-coping strategy during the pandemic. There is a need for the introduction of stress management programs to help foster healthy coping skills. Students are important resources for our health system and society and will continue to be vital long term. It is now up to both nursing educators and health administrators to identify and implement the needed improvements in training and safety measures because they are essential for the health of the patient as well as future pandemics.
New Insights into the Biology of the Emerging Tembusu Virus
Authors: Hamel Rodolphe , Phanitchat Thipruethai , Wichit Sineewanlaya , Morales Vargas Ronald Enrique , Jaroenpool Jiraporn , Diagne Cheikh Tidiane , Pompon Julien , Missé Dorothée
Published: August 2021

Reported for the first time in 1955 in Malaysia, Tembusu virus (TMUV) remained, for a long time, in the shadow of flaviviruses with human health importance such as dengue virus or Japanese encephalitis virus. However, since 2010 and the first large epidemic in duck farms in China, the threat of its emergence on a large scale in Asia or even its spillover into the human population is becoming more and more significant. This review aims to report current knowledge on TMUV from viral particle organization to the development of specific vaccines and therapeutics, with a particular focus on host-virus interactions.
Titania Nanosheet Generates Peroxynitrite-Dependent S-Nitrosylation and Enhances p53 Function in Lung Cancer Cells
Authors: Soonnarong Rapeepun , Tungsukruthai Sucharat , Nutho Bodee , Rungrotmongkol Thanyada , Vinayanuwattikun Chanida , Maluangnont Tosapol , Chanvorachote Pithi
Published: August 2021

Metal nanomaterials can enhance the efficacy of current cancer therapies. Here, we show that Ti0.8O2 nanosheets cause cytotoxicity in several lung cancer cells but not in normal cells. The nanosheet-treated cells showed certain apoptosis characteristics. Protein analysis further indicated the activation of the p53-dependent death mechanism. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses revealed the cellular uptake of the nanosheets and the induction of cell morphological change. The nanosheets also exhibited a substantial apoptosis effect on drug-resistant metastatic primary lung cancer cells, and it was found that the potency of the nanosheets was dramatically higher than standard drugs. Ti0.8O2 nanosheets induce apoptosis through a molecular mechanism involving peroxynitrite (ONOO−) generation. As peroxynitrite is known to be a potent inducer of S-nitrosylation, we further found that the nanosheets mediated the S-nitrosylation of p53 at C182, resulting in higher protein-protein complex stability, and this was likely to induce the surrounding residues, located in the interface region, to bind more strongly to each other. Molecular dynamics analysis revealed that S-nitrosylation stabilized the p53 dimer with a ΔGbindresidue of <−1.5 kcal/mol. These results provide novel insight on the apoptosis induction effect of the nanosheets via a molecular mechanism involving S-nitrosylation of the p53 protein, emphasizing the mechanism of action of nanomaterials for cancer therapy.
High-Precision Time-Frequency Signal Simultaneous Transfer System via a WDM-Based Fiber Link
Authors: Zang Qi , Quan Honglei , Zhao Kan , Zhang Xiang , Deng Xue , Xue Wenxiang , Chen Faxi , Liu Tao , Dong Ruifang , Zhang Shougang
Published: August 2021

In this paper, we demonstrate a wavelength division multiplexing (WDM)-based system for simultaneously delivering ultra-stable optical frequency reference, 10 GHz microwave frequency reference, and a one pulse per second (1 PPS) time signal via a 50 km fiber network. For each signal, a unique noise cancellation technique is used to maintain their precision. After being compensated, the transfer frequency instability in terms of the overlapping Allan deviation (OADEV) for the optical frequency achieves 2 × 10−17/s and scales down to 2 × 10−20/10,000 s, which for the 10 GHz microwave reference, approaches 4 × 10−15/s and decreases to 1.4 × 10−17/10,000 s, and the time uncertainty of the 1 PPS time signal along the system is 2.08 ps. In this scheme, specific channels of WDM are, respectively, occupied for different signals to avoid the possible crosstalk interference effect between the transmitted reference signals. To estimate the performance of the above scheme, which is also demonstrated in this 50 km link independent of these signals, the results are similar to that in the case of simultaneous delivery. This work shows that the WDM-based system is a promising method for building a nationwide time and frequency fiber transfer system with a communication optical network.
Quantum Chaos in Time Series of Single Photons as a Superposition of Wave and Particle States
Authors: Mendoza Benjamín David , Lara Diego Alberto , López-Aparicio Jehú , Armendáriz Gustavo , López-Hernández Leonardo , Velázquez Víctor , Hernández Edna Magdalena , Grether Marcela , López-Moreno Enrique , Frank Alejandro
Published: August 2021

We build a time series of single photons with quantum chaos statistics, using a version of the Grangier anti-correlation experiment. The criteria utilized to determine the presence of quantum chaos is the frame of the Fano factor and the power spectrum. We also show that photons with chaotic statistics are in a balanced superposition of photons with both wave-like and particle like behaviors. To support the presence of quantum chaos, we study both Shannon’s entropy, and the complexity of single photons time series.
Pressurized Hot Water Extraction of Okra Seeds Reveals Antioxidant, Antidiabetic and Vasoprotective Activities
Authors: Ong Eng Shi , Oh Christina Liu Ying , Tan Joseph Choon Wee , Foo Su Yi , Leo Chen Huei
Published: August 2021

Abelmoschus esculentus L. Moench (okra) is a commonly consumed vegetable that consists of the seeds and peel component which are rich in polyphenolic compounds. The aim of this study is to utilize pressurized hot water extraction (PHWE) for the extraction of bioactive phytochemicals from different parts of okra. A single step PHWE was performed at various temperatures (60 °C, 80 °C, 100 °C and 120 °C) to determine which extraction temperature exhibits the optimum phytochemical profile, antioxidant and antidiabetic activities. The optimum temperature for PHWE extraction was determined at 80 °C and the biological activities of the different parts of okra (Inner Skin, Outer Skin and Seeds) were characterized using antioxidant (DPPH and ABTS), α-glucosidase and vasoprotective assays. Using PHWE, the different parts of okra displayed distinct phytochemical profiles, which consist of primarily polyphenolic compounds. The okra Seeds were shown to have the most antioxidant capacity and antidiabetic effects compared to other okra parts, likely to be attributed to their higher levels of polyphenolic compounds. Similarly, okra Seeds also reduced vascular inflammation by downregulating TNFα-stimulated VCAM-1 and SELE expression. Furthermore, metabolite profiling by LC/MS also provided evidence of the cytoprotective effect of okra Seeds in endothelial cells. Therefore, the use of PHWE may be an alternative approach for the environmentally friendly extraction and evaluation of plant extracts for functional food applications.
Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production
Authors: Krstić-Milošević Dijana , Banjac Nevena , Janković Teodora , Vinterhalter Dragan , Vinterhalter Branka
Published: August 2021

In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.
Linear Drive Based on Silicon/Ethanol Composite
Authors: Kapłon Tomasz , Milecki Andrzej
Published: August 2021

The paper presents a concept of an actuator, based on a silicon/ethanol composite placed in the brass bellows. Such actuator is operating based on a change in the physical state of ethanol, which is enclosed in bubbles surrounded by a matrix of silicone rubber. In this paper, the prototype of the actuator is described, and a series of its test results, in the open and closed loops, are presented. Two laser distance-sensors, with different accuracies, were used as a source of the feedback signal. During the investigations the temperature of the actuator was also measured. This has allowed us to determine the delay in heat flow from the heater to the composite. In the closed loop, P- and PI-type controllers were used in the drive positioning experiments. It was discovered that in the closed loop control, it was possible to achieve a positioning error of less than 200 µm. During the tests, the temperature inside the drive and the ambient temperature were also measured. In order to improve the dynamics of the drive, a small fan was used, controlled by the automation system. It allowed us to shorten the time to return the drive to its starting position. The results of frequency tests of the drive have also been presented.
Green and Healthier Alternatives to Chemical Additives as Cheese Preservative: Natural Antimicrobials in Active Nanopackaging/Coatings
Authors: Lima Rayssa Cruz , Carvalho Anna Paula Azevedo de , Vieira Carla P. , Moreira Rodrigo Vilela , Conte-Junior Carlos Adam
Published: August 2021

The side effects and potential impacts on human health by traditional chemical additives as food preservatives (i.e., potassium and sodium salts) are the reasons why novel policies are encouraged by worldwide public health institutes. More natural alternatives with high antimicrobial efficacy to extend shelf life without impairing the cheese physicochemical and sensory quality are encouraged. This study is a comprehensive review of emerging preservative cheese methods, including natural antimicrobials (e.g., vegetable, animal, and protist kingdom origins) as a preservative to reduce microbial cheese contamination and to extend shelf life by several efforts such as manufacturing ingredients, the active ingredient for coating/packaging, and the combination of packaging materials or processing technologies. Essential oils (EO) or plant extracts rich in phenolic and terpenes, combined with packaging conditions and non-thermal methods, generally showed a robust microbial inhibition and prolonged shelf life. However, it impaired the cheese sensory quality. Alternatives including EO, polysaccharides, polypeptides, and enzymes as active ingredients/nano-antimicrobials for an edible film of coating/nano-bio packaging showed a potent and broad-spectrum antimicrobial action during shelf life, preserving cheese quality parameters such as pH, texture, color, and flavor. Future opportunities were identified in order to investigate the toxicological effects of the discussed natural antimicrobials’ potential as cheese preservatives.
Cobalt-Mediated Radical Copolymerization of Vinylidene Fluoride and 2,3,3,3-Trifluoroprop-1-ene
Authors: Falireas Panagiotis G. , Ameduri Bruno
Published: August 2021

New copolymers based on vinylidene fluoride (VDF) and 2,3,3,3-tetrafluoroprop-1-ene (1234yf) were synthesized by organometallic-mediated radical copolymerization (OMRcP) using the combination of bis(tert-butylcyclohexyl) peroxydicarbonate initiator and bis(acetylacetonato)cobalt(II), (Co(acac)2) as a controlling agent. Kinetics studies of the copolymerization of the fluoroalkenes copolymers were monitored by GPC and 19F NMR with molar masses up to 12,200 g/mol and dispersities (Đ) ranging from 1.33 to 1.47. Such an OMRcP behaves as a controlled copolymerization, evidenced by the molar mass of the resulting copolymer-monomer conversion linear relationship. The reactivity ratios, ri, of both comonomers were determined by using the Fineman-Ross and Kelen-Tüdos fitting model leading to rVDF = 0.384 ± 0.013 and r1234yf = 2.147 ± 0.129 at 60 °C, showing that a lower reactivity of VDF integrated in the copolymer to a greater extent leads to the production of gradient or pseudo-diblock copolymers. In addition, the Q (0.03) and e (0.06 and 0.94) parameters were assessed, as well as the dyad statistic distributions and mean square sequence lengths of PVDF and P1234yf.
Multifunctional and Self-Healable Intelligent Hydrogels for Cancer Drug Delivery and Promoting Tissue Regeneration In Vivo
Authors: Pishavar Elham , Khosravi Fatemeh , Naserifar Mahshid , Rezvani Ghomi Erfan , Luo Hongrong , Zavan Barbara , Seifalian Amelia , Ramakrishna Seeram
Published: August 2021

Regenerative medicine seeks to assess how materials fundamentally affect cellular functions to improve retaining, restoring, and revitalizing damaged tissues and cancer therapy. As potential candidates in regenerative medicine, hydrogels have attracted much attention due to mimicking of native cell-extracellular matrix (ECM) in cell biology, tissue engineering, and drug screening over the past two decades. In addition, hydrogels with a high capacity for drug loading and sustained release profile are applicable in drug delivery systems. Recently, self-healing supramolecular hydrogels, as a novel class of biomaterials, are being used in preclinical trials with benefits such as biocompatibility, native tissue mimicry, and injectability via a reversible crosslink. Meanwhile, the localized therapeutics agent delivery is beneficial due to the ability to deliver more doses of therapeutic agents to the targeted site and the ability to overcome post-surgical complications, inflammation, and infections. These highly potential materials can help address the limitations of current drug delivery systems and the high clinical demand for customized drug release systems. To this aim, the current review presents the state-of-the-art progress of multifunctional and self-healable hydrogels for a broad range of applications in cancer therapy, tissue engineering, and regenerative medicine.
Design and Analysis of a Mooring Buoy for a Floating Arrayed WEC Platform
Authors: Boo Sung Youn , Shelley Steffen Allan
Published: August 2021

This paper presents the design and analysis of a mooring buoy and its mooring systems to moor a floating platform mounting an arrayed Wave Energy Converters (WECs). The mooring buoy allows the WEC platform to weathervane around the mooring buoy freely by the prevailing environment directions, which enables consistent power generation. The WEC platform is connected to the buoy with synthetic hawsers, while station-keeping of the buoy is maintained with catenary mooring lines of chains tied to the buoy keel. The buoy also accommodates a power cable to transfer the electricity from the WEC platform to the shore. The WEC platform is designed to produce a total of 1.0 MW with multiple WECs installed in an array. Fully coupled time-domain analyses are conducted under the site sea states, including extreme 50 y and survival 100 y conditions. The buoy motions, mooring tensions and other design parameters are evaluated. Strength and fatigue designs of the mooring systems are validated with requirements according to industry standards. Global and local structural designs of the mooring buoy are carried out and confirm the design compliances.
The Everyday Power of Liturgy: On the Significance of the Transcendental for a Phenomenology of Liturgy
Authors: DeRoo Neal
Published: August 2021

The task of this article is to articulate the everyday power of liturgy by clarifying the transcendental significance of ritual action. The paper makes three major claims: first, that liturgical practices function transcendentally, and therefore alter how we experience the world; second, that liturgical practices therefore exercise an immense formative power in our everyday living, including the power to open up or close down the possibility of encountering the sacred in our everyday lives; third, that this power of liturgy can be articulated theoretically through a transcendental phenomenological approach, thereby suggesting that a rigorous phenomenology of liturgy must necessarily include a transcendental element.