bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Fascial Connectivity in Musculoskeletal Dysfunctions: A Narrative Review
Authors: Ajimsha M.S. , Shenoy Pramod D. , Gampawar Neeraj
Published: May 2020

Introduction: Musculoskeletal dysfunctions happen to be the most common reason for referral to physiotherapy and manual therapy services. Therapists may use several articular and/or soft tissue concepts/approaches to evaluate and treat such dysfunctions that may include integration of myofascial system. Despite the research in this area spanning more than three decades, the role played by fascia has not received its duly deserved attention, owing to the lack of definitive research evidence. The concept of ‘fascial connectivity’ evolved two decades ago from a simple anatomical hypothesis called ‘myofascial meridians’. Since then it has been widely researched, as conceptually it makes more sense for functional movements than ‘single-muscle’ theory. Researchers have been exploring its existence and role in musculoskeletal dysfunctions and clinicians continue to practice based on anecdotal evidence. This narrative review attempts to gather available evidence, in order to support and facilitate further research that can enhance evidence based practice in this field. Methods: A search of most major databases was conducted with relevant keywords that yielded 272 articles as of December 2019. Thirty five articles were included for final review with level of evidence ranging from 3b to 2a (as per Center of Evidence Based Medicine’s scoring). Results: Findings from cadaveric, animal and human studies supports the claim of fascial connectivity to neighboring structures in the course of specific muscle-fascia chains that may have significant clinical implications. Current research (level 2) supports the existence of certain myofascial connections and their potential role in the manifestation of musculoskeletal dysfunctions and their treatment. Conclusion: Although these reviews and trials yield positive evidence for the objective reality/existence of fascial connectivity and continuity, several aspects need further exploration and in-depth analysis, which could not be evidenced entirely in this review. Manual and physical therapists may utilize the concept of fascial connectivity as a convincing justification to deal with clinical problems, but need to remain vigilant that functional implications are still being investigated.
Fighting the SARS CoV-2 (COVID-19) Pandemic with Soap
Authors: Chaudhary Narendra Kumar , Chaudhary Nabina , Dahal Manis , Guragain Biswash , Rai Sumie , Chaudhary Rahul , Sachin K.M. , Lamichhane-Khadka Reena , Bhattarai Ajaya
Published: May 2020

Today, the entire globe is struggling to deal with the greatest pandemic of the century, COVID-19. With no clinically approved treatments available, we are left with no options other than following the preventive measures issued by the World Health Organization (WHO). Among many others, hand washing with soap and water has been emphasized the most because it is cost-effective and easily accessible to the general public. Various studies have reported that soaps offer unique chemical properties that can disinfect the virus as a whole. However, there is still ambiguity in the general public about whether soaps can really shield us from this highly contagious disease. In an attempt to help eliminate the ambiguity, we analyzed the mechanisms underlying the efficacy of soap and its prospect for preventing the spread of COVID-19. In this paper, we have provided an overview of the history and characteristics of SARS-CoV-2 (COVID-19), the detailed mechanisms of the deactivation of viruses by soaps, and the potential effectiveness of soap in eliminating coronaviruses including SARS-CoV-2.
The Influence of Nonpharmacological Complex Therapy Conducted at Community Day-Care Center on the Cognitive Functions and Mood in Older Adults
Authors: Gorzkowska Agnieszka , Zacharska-Quiaum Izabela , Cholewa Joanna , Cholewa Jarosław
Published: May 2020

Nonpharmacological therapeutic interventions in elderly may lead to the reduction of cognitive and depressive symptoms. The aim of the study was to evaluate changes in cognitive functions and mood er or not. in older adults participating in therapy, conducted in the community day-care center (CD-CC). 46 elderly adults (21 M, 25 W) (SG) were examined. The control group (CG) included 45 adults (12 M, 33 W), who participated in the activities of the University of the Third Age (U3A). The following measuring tools were used: Mini-Mental State Examination (MMSE), Clock-Drawing Test (CDT), Verbal Fluency Test (VFT), Digit Span Test (DST), Stroop Color and Word Test (SCWT), Beck's Depression Inventory (BDI), and Hospital Anxiety and Depression Scale (HADS). The intervention consisted of CD-CC 6-month complex therapy. In the SG, compared to the CG, the scores on the: MMSE, CDT, VFT, DST, and SCWT were significantly lower (p<0,05), and BDI was significantly higher (p<0,05). After intervention, the SG and the CG, did not show substantial differences in their scores on the: MMSE, CDT, and BDI. In the SG, a significant improvement (p<0,05) was reported on the: VFT, BDI, and HADS scores. The CD-CC complex therapy can be helpful for the cognitive and emotional elderly functioning.
Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nuts by Real Time PCR
Authors: Sanchiz Africa , Cuadrado Carmen , Haddad Joseph , Linacero Rosario
Published: May 2020

Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitive population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut; pistachio and cashew allergens have been tested. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment, in different extent depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow detecting up to 1000 ppm of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices
COVID-19: Multiple Diseases Simulating Extreme High-Altitude Exposure? Oxygen Transport Physiology and Scarce Need of Ventilators; Andean Condor’s-Eye-View
Authors: Zubieta-Calleja Gustavo R. , Zubieta-DeUrioste Natalia , Venkatesh Thuppil , Das Kusal , Soliz Jorge
Published: May 2020

The critical hypoxia in COVID-19 patients during this pandemic, has taken away many lives all around the globe. The mechanism has been poorly understood and initially, word got around that it was a SARS (Severe Acute Respiratory Syndrome) pneumonia. The atypical images in lung computerized axial tomography (CAT) scans were alarming. This immediately alerted everyone including poor countries to purchase lacking sophisticated ventilator equipment. However, in some countries, even 88% of the patients on ventilators lost their lives. New observations and pathological findings are gradually clarifying the disease. What seems evident is that it is not only one disease but several, with different responses in different countries and different altitudes. The critical hypoxia and «gasping» present in some patients are not totally understood. It was mentioned that it could be like a High-Altitude Pulmonary Edema (HAPE). Hereby, as high-altitude medicine and hypoxia physiology specialists, we compare the pathophysiology with that of high-altitude exposure in order to understand the mechanisms involved. Some differences in lung radiological images along with transmission and viral attack mechanisms are discussed. The oxygen transport triad used at high-altitude can be applied on this pathology in order to propose even the use of erythropoietin (EPO) early in the treatment. The immune system is the most important long-term survival tool, so we suggest a short-term strategy: the use of special Earth open-circuit astronaut-resembling suits with effective outside air filtering re-breathing mechanisms in order to return to work and daily activities, without contamination risk. Thereby, the curve can be flattened without quarantine and the economy could recover.
Covid-19 Situation in Bangladesh
Authors: Mohiuddin Abdul Kader
Published: May 2020

COVID-19 has affected 210 countries around the world, killed more than 240,000 and infected more than 3.4 million. Bangladesh, a South Asian low-middle-income economy, has experienced a demographic and epidemiological transition with rapid urbanization and a gradual increase in life expectancy. It is the seventh most populous country in the world and population of the country is expected to be nearly double by 2050. The increasing burden of noncommunicable diseases in Bangladesh can be attributable to rapid urbanization and nearly 50% of all slum dwellers of the country live in Dhaka division. In 2017, National Rapid Response Team of IEDCR investigated 26 incidents of disease outbreak. The joint survey of the Power and Participation Research Centre and BRAC Institute of Governance and Development reveals that per capita daily income of urban slum and rural poor drops by 80% due to present countrywide shutdown enforced by the government to halt the spread of Covid-19. 40%-50% of these population took loans to meet the daily expenses.
Effect of Conventional and Minimum Tillage, Combine Use of Organic Manure and Synthetic Based Fertilizers with Foliar Spray of Zinc Sulphate for Sustaining Wheat Productivity, Quality and Status of Soil Fertility
Authors: Kumar Manoj , Singh Vikram , Kumari Km. Nikam , KUMAR SAGAR , Nandan Rajiv , Abraham Thomas
Published: May 2020

Intensive cultivation and excess chemical fertilizer continuous tillage activity have shown that environmental, soil & water degradation in South Asia has produced serious problems in soil health or agricultural development with a rise in population. The desire for renewable alternatives has therefore been underestimated. The effect on cropping efficiency, machine productivity and development economy of two tillage cum (Vermicompost and FYM manure, Urea, and DAP) or two facts regarding the application of foliar spray and without zinc fertilizers were evaluated. Conventional tillage (1 ploughing by tractor drawn disc plough + 2 harrowing + 1 fb cultivator with planking) increased grain yield (10.85 percent) and (7.21), both during the first and second years of experimental plots, over minimum tillage (1 ploughing by tractor drawn rotavator) (MT). The improved grain output of wheat was primarily due to increased tiller development in CT treatments during both the years. Similarly, maximum most of the growth attributes, productivity components and physical quality charcters were recorded in CT tillage practice. In the joint management of inorganic or organic nitrogen, significant and maximum grain (4.63 t/ha) and straw (8.97 t/ha) yield, weight of spike (2.68 g), length of grain (6.49 mm), breadth of grain (3.29 mm) and length: breadth ratio of grain (1.98) were recorded during 2015-16, However purely chemical fertilizer applied treatment recorded during first year significant and maximum number of tillers per hill (3.14), leaf area index (2.31), plant dry matter (13.09 g plant-1), grain yield (4.00 t ha-1), straw yield (7.87 t ha-1), number of grains per spike (42.42) and weight of spike (2.62 g), respectively.Soil cultivation by CT with fertilized fertilizer at the prescribed total dose of nitrogen 120 kg/ha, in which ½ nitrogen through organic (Vermicompost 3000 kg/ha) and ½ through inorganic (Urea, 117.69 kg/ha and (Diammonium phosphate (DAP) 32.60 kg/ha) during second year with foliar applications of zinc (2.50 kg/ha) were found to increase crop productivity and resilience in management.Soil chemical quality status increased after second year of experiment in combined used of MT (1 ploughing by tractor drawn rotavator) and organic (Vermicompost 4500 kg/ha) and inorganic fertilization ½ through inorganic (Urea, 44.16 kg/ha and (Diammonium phosphate (DAP) 48.91kg/ha) with foliar applications of zinc (2.50 kg/ha).
4D Einstein-Lovelock Black Holes: Hierarchy of Orders in Curvature
Authors: Konoplya Roman A. , Zhidenko Alexander
Published: May 2020

The Einstein-Lovelock theory contains an infinite series of corrections to the Einstein term with an increasing power of the curvature. It is well-known that for large black holes the lowest (Gauss-Bonnet) term is the dominant one, while for smaller black holes higher curvature corrections become important. We will show that if one is limited by positive values of the coupling constants, then the dynamical instability of black holes serves as an effective cut-off of influence of higher curvature corrections in the 4D Einstein-Lovelock approach: the higher is the order of the Lovelock term, the smaller is the maximal value of the coupling constant allowing for stability, so that effectively only a first few orders can deform the observable values seemingly. For negative values of coupling constants this is not so, and, despite some suppression of higher order terms also occurs due to the decreasing threshold values of the coupling constant, this does not lead to an noticeable opportunity to neglect higher order corrections. In the case a lot of orders of Lovelock theory are taken into account, so that the black-hole solution depends on a great number of coupling constants, we propose a compact description of it in terms of only two or three parameters encoding all the observable values.
Antimycobacterial Activity of Laurinterol and Aplysin from Laurencia johnstonii
Authors: García-Davis Sara , Leal-López Karla , Molina-Torres Carmen A. , Vera-Cabrera Lucio , Díaz-Marrero Ana R. , Fernández José J. , Carranza-Rosales Pilar , Viveros-Valdez Ezequiel
Published: May 2020

Marine environments represent a great opportunity for the discovery of compounds with a wide spectrum of bioactive properties. Due to the privileged conditions of natural selection, marine natural products are subject to overcome the pressure put on identify novel drugs; not only in the case of newly discovered bioactive metabolites, but also in those previously known. Since drug resistance has caused an increase in infections caused by tuberculous and nontuberculous Mycobacteria, the re-evaluation of known bioactive metabolites has been suggested as a means to address this problem. In this sense, this study presents an evaluation of in vitro effect of laurinterol (1) and aplysin (2), two brominated sesquiterpenes isolated from Laurencia johnstonii against nine Mycobacterium tuberculosis strains and six nontuberculous mycobacteria. Laurinterol (1) exhibited good anti-tuberculous activity, especially against nontuberculous mycobacteria, being remarkable the effect against M. abscessus with MIC values lower than the reference drug imipenem. This study provides further evidence for the antimycobacterial activity of some sesquiterpenes from L. johnstonii, that can be considered an interesting lead compound for the discovery of novel antimycobacterial molecules to treat NTM infections.
Analytical Descriptions of High-Tc Cuprates by Introducing Rotating Holes and a New Model to Handle Many-Body Interactions
Authors: Ishiguri Shinichi
Published: May 2020

This paper describes all the properties of high-Tc cuprates by introducing rotating holes which are created by angular momentum conservations on a two dimensional CuO2 surface, and which have a different mass from that of a normal hole due to the magnetic field energy induced by the rotation. This new particle called a macroscopic boson describes doping dependences of pseudo gap temperature and the transition temperature at which an anomaly metal phase appears. In addition, it also describes all the properties of the anomaly metal phase, using findings from our previous article [1] . Furthermore, the present paper introduces a new model to handle many-body interactions, which results in a new statistic equation. A partition function of macroscopic bosons describes all the properties of the anomaly metal phase, which sufficiently agrees with experiments. Moreover, the above-mentioned statistic equation describing many-body interactions accurately explains why high-Tc cuprates have significantly high critical temperatures, which indicates that the source of the characteristic stems from pseudo gap energy. By introducing a macroscopic boson and the new statistic model for many-body interactions, the present paper uncovered the mystery of high-Tc cuprates, which have been a challenge for many researchers. Moreover, in the present paper, pure analytical calculations are conducted. These calculations agree with experimental data which do not employ numerical calculations or fitting methods but employ many actual physical constants.
Studying Phase Behavior of Oil/Natural-Gas Systems for Designing Gas-Injection Operations: A Montney Case Study
Authors: Tran Son , Eghbali Sara , Dehghanpour Hassan
Published: May 2020

Advances in horizontal drilling and multistage hydraulic fracturing have unlocked tight-oil resources, such as the Montney Formation in the Western Canadian Sedimentary Basin. However, the average oil-recovery factor after primary production is 5 to 10% of the original oil in place. The aims of this study are to investigate phase behavior and to estimate the minimum miscibility pressure (MMP) of the Montney oil/natural-gas systems.

First, we measure the MMPs of the oil/gas systems using the vanishing interfacial tension (VIT) technique. The gas samples are methane (C1) and mixtures of methane and ethane (C1/C2). Second, we perform constant-composition-expansion (CCE) tests to study the phase behavior of the oil/gas systems using a pressure/volume/temperature (PVT) cell. To complement the VIT and CCE tests, we perform bulk-phase tests to visualize vaporizing/condensing phenomena at the oil/gas interface using a visualization cell. Finally, we use the measured CCE and MMP data to calibrate the Peng-Robinson (Robinson and Peng 1978) equation of state (PR-EOS) and predict the MMP of the oil/gas systems using ternary diagrams. The results suggest that the dominant mechanism for developing miscibility conditions for oil/C1 and oil/C1/C2 systems is vaporizing and condensing gas drive, respectively. According to the results of the VIT and CCE tests, increasing C2 mole fraction in the gas mixtures significantly reduces MMP of the oil/gas system (from 4,366 psi for oil/C1 to 1,467 psi for oil/C1/C2 with 71.3 mol% C2) and increases the oil-swelling factor (from 1.47 to 1.61 by increasing C2 mol% from 0 to 70 mol%). The results of visualization tests show that the presence of C2 in the injection gas significantly enhances oil swelling compared with the pure-C1 case. We observe vaporizing flows of oil components in all tests and strong condensing flows of C1 and C2 into the oil phase in the C1/C2 test with increasing gas-injection pressure. The MMP values predicted by plotting two-phase equilibrium data on ternary diagrams appear to be in good agreement with the measured ones. The results can be used to optimize the injection-gas composition and operating pressure in the Montney.
Radial peripapillary capillary density in superior segmental optic hypoplasia measured with OCT angiography.
Authors: Abe Maiko , Omodaka Kazuko , Kikawa Tsutomu , Nakazawa Toru
Published: May 2020

Background: To investigate the diagnostic power of radial peripapillary capillary (RPC) density, measured with optical coherence tomography angiography (OCT-A), in patients with superior segmental optic hypoplasia (SSOH).Methods: Forty subjects with SSOH and 40 age- and axial length-matched control subjects were retrospectively registered for this study. SSOH was defined as intraocular pressure less than 21 mmHg with the presence of two of the following: superior rim thinning, superior entrance of the central retinal artery, scleral halo, and pale optic disc; as well as non-progressive visual field loss. RPC density was measured with swept-source OCT-A (Triton, Topcon) overall, in the quadrants, and in the 12 clock-wise sectors. Changes in RPC density were also compared in SSOH patients and age-matched patients with mild- or moderate-stage of glaucoma. RPC density was compared in pairs of groups with Welch’s t-test. Diagnostic power was assessed with the area under the receiver operating characteristics curve (AUC). Results: Overall cpRNFLT was significantly different in the normal (106.7 ± 9.5 μm) and SSOH (77.2 ± 13.7 μm, p < 0.001) subjects. RPC density overall and in the superior, nasal, and inferior quadrants was significantly lower in the SSOH group (all, p < 0.001), but not in the temporal (p = 0.756) quadrant. The diagnostic power of RPC density was highest in the superior quadrant (AUC = 0.928) and the 1 o’clock sector (0.896). Comparing the SSOH and glaucoma patients showed that there were no significant differences in RPC density either overall (p=0.391) or in the superior quadrant (p = 0.268), while RPC density was significantly higher in the inferior (p = 0.005) and temporal quadrants (p < 0.001) and lower in the nasal quadrant (p = 0.029).Conclusions: Low RPC density was found in the three non-temporal quadrants of the optic nerve head in SSOH patients, in comparison to normal subjects. Regionally, RPC density in SSOH was lower in the nasal quadrant and higher in the inferior and temporal quadrants in comparison to glaucoma patients. Measuring RPC density with OCT-A may help the diagnosis of SSOH and may improve the management of glaucoma.
Challenges of recruiting emergency department patients to a qualitative study: a thematic analysis of researchers’ experiences
Authors: Price Delyth , Edwards Michelle , Carson-Stevens Andrew , Cooper Alison , Davies Freya , Evans Bridie , Hibbert Peter , Hughes Thomas , Rainer Timothy , Siriwardena Niro , Edwards Adrian
Published: May 2020

Background: At times of increasing pressure on emergency departments, and the need for research into different models of service delivery, little is known about how to recruit patients for qualitative research in emergency departments. We report from one study which aimed to collect evidence on patients’ experiences of attending emergency departments with different models of general practitioners, but faced challenges in recruiting patients. This paper aims to identify and reflect on the challenges faced at all stages of patient recruitment, from identifying and inviting eligible patients, consenting them for participation and finally to engaging them in interviews and make recommendations based on our learning. Methods: A thematic analysis was carried out on field-notes taken during research visits and meeting minutes of discussions to review and improve patient recruitment throughout the study. Results: The following factors influenced the success of patient recruitment in the emergency department setting: complicated or time-consuming electronic health record systems for identifying patients; narrow participant eligibility criteria; limited research nurse support; and lack of face-to-face communication between researchers and eligible patients. Conclusions: This paper adds to the evidence for improving patient recruitment in different settings, with a focus on qualitative research in emergency departments. Our findings have implications for future studies attempting to recruit patients in similar settings.
Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia
Authors: Ma Xiuqing , Li Yanqin , Liang Yuan , Liu Yang , Yu Ling , Li Chunsun , Liu Qiqi , Chen Liangan
Published: May 2020

Background: Rapid identification of pathogenic bacteria is important for appropriate antimicrobial therapy of pneumonia, but traditional bacteria culture is time-consuming and laborious. The aim of this study was to develop and evaluate a DNA microarray assay for the simultaneously detection of fifteen bacteria species directly from respiratory tract specimens in patients with pneumonia. These species included Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Mycoplasma pneumoniae, Enterococcus faecalis, Enterococcus faecium, Enterobacter cloacae, Stenotrophomonas maltophilia, Burkholderia cepacia, Legionella pneumophila and Chlamydia pneumoniae. The 16S rDNA and specific genes of each pathogen were chosen as the amplification target, amplified with multiplex polymerase chain reaction (PCR), and hybridized to the oligonucleotide probes on the microarray. Results: The DNA microarray can reach a detection limit of 10 3 copies/μL. Nineteen standard strains and 119 clinical isolates were correctly detected with our microarray and 3 non-target species from 4 clinical isolates were not detected. Meanwhile, bacterial pathogens were accurately identified when two or three bacterial targets were mixed together. Furthermore, the results of 99.4% (156/157) clinical specimens were the same to that from the conventional assay. Conclusions: we developed a DNA microarray that could simultaneously detect various bacterial pathogens in pneumonia. The method described here has the potential to provide considerable labor and time savings due to its ability to screen for 15 bacterial pathogens simultaneously.
Mitochondria transfer enhances proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cell and promotes bone defect healing
Authors: Guo Yusi , Chi Xiaopei , Wang Yifan , Heng Boon Chin , Wei Yan , Zhang Xuehui , Zhao Han , Yin Ying , Deng Xuliang
Published: May 2020

Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is considered a promising therapeutic approach for bone defect repair. However, during the transplantation procedure, the functions and viability of BMSCs may be impaired due to extended durations of in vitro culture, aging and disease conditions of patients. Inspired by spontaneous intercellular mitochondria transfer that naturally occurs within injured tissues to rescue cellular or tissue function, we investigated whether artificial mitochondria transfer into pre-transplant BMSCs in vitro could improve cellular function and enhance their therapeutic effects on bone defect repair in situ . Methods: Mitochondria were isolated from donor BMSCs and transferred into recipient BMSCs of the same batch and passage. Subsequently, changes in proliferative capacity and cell senescence were evaluated by live cell imaging, Cell Counting Kit-8 assay, cell cycle analysis, Ki67 staining, qPCR and Western Blot analysis of C-myc expression, and β-galactosidase staining. Migration ability was evaluated by the transwell migration assay, wound scratch healing and cell motility tests. Alakine phosphatase (ALP) staining, Alizarin Red staining, combined qPCR and Western Blot analyses of Runx2 and BMP2 were performed to elucidate the effects of mitochondria transfer on the osteogenic potential of BMSCs in vitro . After that, in vivo experiments were performed by transplanting mitochondria-recipient BMSCs into a rat cranial critical-size bone defect model. Micro CT scanning and histological analysis were conducted at 4 and 8 weeks after transplantation to evaluate osteogenesis in situ . Finally, in order to establish the correlation between cellular behavioral changes and aerobic metabolism, OXPHOS (oxidative phosphorylation) and ATP production were assessed and inhibition of aerobic respiration by oligomycin was performed. Results: Mitochondria-recipient BMSCs exhibited significantly enhanced proliferation and migration, and increased osteogenesis upon osteogenic induction. The in vivo results showed more new bone formation after transplantation of mitochondria-recipient BMSCs in situ . Increased OXPHOS activity and ATP production were observed, which upon inhibition by oligomycin attenuated the enhancement of proliferation, migration and osteogenic differentiation induced by mitochondria transfer. Conclusions: Mitochondria transfer is a feasible technique to enhance BMSCs function in vitro and promote bone defect repair in situ through the up-regulation of aerobic metabolism. The results indicated that mitochondria transfer may be a novel promising technique for optimizing stem cell therapeutic function.
Relationships between abnormal neural activities and cognitive impairments in patients with drug-naive first-episode schizophrenia
Authors: Yan Wei , Zhang Rongrong , Zhou Min , Lu Shuiping , Li Wenmei , Xie Shiping , Zhang Ning
Published: May 2020

Background Prior resting state functional magnetic resonance imaging studies via the regional homogeneity (ReHo) method have demonstrated inconsistent and conflicting results because of several confounding factors, such as small sample size, medicinal influence, and illness duration. Relationships between ReHo measures and cognitive impairments in patients with drug-naive first-episode schizophrenia (dn-FES) are rarely reported. This study was conducted to explore the correlations between ReHo measures, cognitive deficits, and clinical symptoms in patients with dn-FES. Methods A total of 69 patients with dn-FES and 74 healthy controls were recruited. MATRICS Consensus Cognitive Battery (MCCB), Wechsler Adult Intelligence Scale (WAIS), and Positive and Negative Syndrome Scale (PANSS) were used to assess cognitive function, intelligence quotient (IQ), and clinical symptoms, respectively. The correlations between ReHo map and cognitive deficits, the severity of symptoms were examined using strict correlation analyses within brain areas. Results The ReHo values in the right middle frontal gyrus (MFG) and the superior frontal gyrus (SFG) increased in the dn-FES group, whereas the ReHo values in the right cuneus decreased. Correlation analysis showed that the ReHo values in the right MFG were positively correlated with attention/vigilance impairments, social cognition deficits, the severity of clinical manifestations. Conclusions These findings suggested that abnormal spontaneous activities in the right MFG reflect the illness severity and cognitive deficits, which serves as a basis for establishing the objective diagnostic markers and might be a clinical intervention target for patients with schizophrenia.
Regional SUV quantification in hybrid PET/MR, a comparison of two atlas-based automatic brain segmentation methods
Authors: Ruan Weiwei , Sun Xun , Hu Xuehan , Liu Fang , Hu Fan , Guo Jinxia , Zhang Yongxue , Lan Xiaoli
Published: May 2020

Background：Quantitative analysis of brain positron-emission tomography (PET) depends on structural segmentation, which can be time-consuming and operator-dependent when performed manually. Previous automatic segmentation usually registered subjects’ images onto an atlas template (defined as RSIAT here) for group analysis, which changed the individuals’ images and probably affected regional PET segmentation. In contrast, we could register atlas template to subjects’ images (RATSI), which created an individual atlas template and may be more accurate for PET segmentation. We segmented two representative brain areas in twenty Parkinson disease (PD) and eight multiple system atrophy (MSA) patients performed in hybrid positron-emission tomography/magnetic resonance imaging (PET/MR). The segmentation accuracy was evaluated using the Dice coefficient (DC) and Hausdorff distance (HD). and the standardized uptake value (SUV) measurements of these two automatic segmentation methods were compared, using manual segmentation as a reference. Results：The DC of RATSI increased and the HD decreased significantly (P < 0.05) compared with the RSIAT in PD, while the results of one-way analysis of variance (ANOVA) found no significant differences in the SUVmean and SUVmax among the two automatic and the manual segmentation methods. Further, RATSI was used to compare regional differences in cerebral metabolism pattern between PD and MSA patients. The SUVmean in the segmented cerebellar gray matter for the MSA group was significantly lower compared with the PD group (P<0.05), which is consistent with previous reports. Conclusion：The RATSI was more accurate for the caudate nucleus and putamen automatic segmentation, and can be used for regional PET analysis in hybrid PET/MR.
Results of the Neuroblastoma-2015 protocol for treating neuroblastoma in China: experience from multiple centers
Authors: Yan Jie , Jin Yan , Li Fu , Jiang Lian , Lu Jun , Li Jianxin , Dai Yunpeng , Zhan Jianghua , Cao Yanna , Li Zhongyuan , Liu Yun , Wang Daowei , Li Zhanglin , Zhao Qiang , Li Jie
Published: May 2020

Background: The collaborative efforts of the Chinese Childhood Cancer Organization (CCCG) have led to an improved understanding of NB biology, standardized classification, and stratification strategies, thereby improving outcomes in China. We aimed to assess the NB-2015 protocol developed by the CCCG in 2015 and to evaluate the degree of impact of autologous peripheral blood stem cell transplantation (APBSCT). Methods: This study enrolled NB patients from seven medical centers in China between 2015 and 2018. In this paper, we retrospectively analyzed the clinical characteristics of 161 patients and used their effect on survival as an outcome measure. Results: The 3-year event-free survival (EFS) rates were 95.5%, 91.6%, and 51.1% in the low-, medium-, and high-risk groups, respectively. In total, 3 out of 7 (42.8%) patients with stage 2B disease, none of whom had MYCN amplifications, relapsed. A total of 47 of the 78 patients in the high-risk group underwent external-beam radiation to the primary tumor bed (dose ranging from 18 to 36 Gy). There was a significant difference in efficacy between patients who received radiation therapy and those who did not (3-year OS P = 0.041). Within the high-risk group, The 3-year EFS rates of the patients with and without APBSCT were 72.6% and 37.1%, respectively (P=0.008).Conclusions: This study showed that satisfactory results were obtained without fatal complications using the NB-2015 protocol. Chemotherapy should be intensified in the stage 2B group. APBSCT can effectively improve the survival rate of NB patients, especially high-risk patients.
A long non-coding RNA signature predicts survival for glioblastoma as prognostic biomarkers
Authors: Li Zhenzhe , Lv Zhonghua , Yu Lei , Zhang Sibin , Wang Yingjie , Ai Siqi , Zhu Kaibin , Li Qingsong , Liang Peng
Published: May 2020

Background: Glioblastoma (GBM) is one of the most fatal tumors in the central nervous system. Its prognosis is very poor. There is increasing evidence that long noncoding RNA (lncRNA) participates in the biological process of glioblastoma. Nevertheless, the role of lncRNA in predicting the prognosis of GBM is still uncertain. Methods: In this study, using RNA-Seq and clinical follow-up data of GBM patients from The Cancer Genome Atlas (TCGA), we performed differential analysis of lncRNA, univariable and multivariable Cox regression analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and Gene Ontology (GO) analysis.Results: We identified four lncRNAs closely interrelated with survival and prognosis of GBM patients. This lncRNA signature was effective in both the training set and the testing set, and it was independent to clinical factors.Conclusions: Our data suggested that the four lncRNAs could be used as promising biomarkers for predicting prognosis in GBM patients.
Effects of anesthetic method on inflammatory response in patients with Parkinson’s disease: a randomized controlled study
Authors: Hwang Won Jung , Joo Min Ah , Joo Jin
Published: May 2020

BackgroundThe pathogenesis of Parkinson’s disease (PD) involves degeneration of dopaminergic neurons, which is influenced by innate and adaptive immunity. IL-17 is a characteristic cytokine secreted by Th17 cells, which acts as a powerful stimulator of neutrophil migration and infiltration and promotes the secretion of inflammatory cytokines. General anesthesia and surgical stress induce immune and inflammatory responses that activate the immunosuppressive mechanism in the perioperative period. The present study investigated changes in levels of inflammatory cytokines, such as IL-17, IL-1β, and TNF-α, in patients with PD undergoing general anesthesia with inhalational anesthetics or TIVA.MethodsAdult patients, aged 40–75 years, scheduled for cerebral stimulator implantation were enrolled. Upon arrival at the operating theater, patients were allocated to the inhalational (I) or TIVA (T) group using block randomization. In group I, anesthesia was induced by tracheal intubation 1–2 min after intravenous administration of propofol (1–2 mg/kg) and rocuronium (0.6–1 mg/kg). Thereafter, anesthesia was maintained with 1–2 vol% sevoflurane, 0.01–0.2 kg/min remifentanil, and O2/air (FiO2 0.4). In group T, propofol (3–6 µg/mL), remifentanil (2–6 ng/mL), and rocuronium (0.6–1 mg/kg) were administered using target controlled infusion (TCI) for induction of anesthesia. Blood samples were obtained preoperatively (T0), 2 h after induction of anesthesia (T1), and 24 h after surgery (T2). IL-17, IL-1β, and TNF-α levels were evaluated by ELISA.ResultsSerum levels of IL-17 were elevated at T2 in group I compared to group T but the difference was not statistically significant. IL-1β tended to be greater in group I compared to group T, but the differences were not significant. (Fig. 3). TNF-α was slightly higher at all time points in group T and showed a tendency to increase at T2 in both groups, but this was not statistically significant (Fig. 4).Conclusions﻿TIVA may be useful for inhibiting neuroinflammation by inhibiting the increase in serum levels of IL-17 24 h after implantation surgery. Serum IL-17 level may be used as a biomarker for PD progression.TRIAL REGISTRATION:Clinical Research Information Service of Korea National Institute of Health (CRIS) Identification number: KCT0002061. Registered 25 October 2019 - Retrospectively registered, https://cris.nih.go.kr/cris/search/search_result_st01.jsp?seq=15125
The impact of aiding robotic-assisted surgery, simulation, virtual reality game-playing and seniority on novices’ learning via skills simulator
Authors: Chen Yin-Yu , Chao Yin-Kai , Huang Yi-Ching , Chao Chung-Hsien , Hsieh Ming-Ju
Published: May 2020

Background

Robotic-assisted surgery learning is highly self-governance, albeit its flourishing. Novices of robotic-assisted surgery are usually trained by virtual reality simulators. This study aims to evaluate whether novices’ prior experiences of aiding robotic-assisted surgery, simulation on virtual reality or game-playing gives weight to their learning outcomes.
Methods

Novices’ attitudes towards robotic-assisted surgery and video game-playing experiences were investigated using a questionnaire. Voluntary novices (n = 70) comprising surgical trainees (first-year to sixth-year residents) and surgeons were then examined on a VR simulator. The simulator automatically generated examination scores. Questionnaires and examination scores were analysed using SPSS.
Results

Participants’ prior experiences of aiding robotic-assisted surgery (p < .01) and having robotic surgical simulation within six months (p < .01) was associated with significantly higher VR simulator performance, but not prior video game-playing (p > .05). Resident participant years 3–5 performed significantly better than resident participant years 1–2, and 6 and visiting staff (p < .01).
Conclusions

When formal robotic surgical training is lacking, novices’ learning can be developed via both simulation and engaging in real cases. Formal robotic surgical training should be ideally introduced during the middle part of the residency period.
Relationship Between the Invasion of Lymphocytes and Cytokines in the Tumor Microenvironment and the Interval After Single Brachytherapy Hypofractionated Rradiotherapy and Conventional Fractionation Radiotherapy in Non-Small Cell Lung Cancer
Authors: Li Lin , Yue Hong Cheng , Han Yun Wei , Liu Wei , Xiong Liang Geng , Zhang Jian Wen
Published: May 2020

Background Lymphocytes and cytokines in tumor microenvironment are the key to immunotherapy, The effect of brachytherapy on tumor microenvironment is not clear. The aim of our study was to analyze the relationship between the invasion of lymphocytes and cytokines in the tumor microenvironment and the interval after single brachytherapy hypofractionated radiotherapy (SBHFRT) and conventional fractionation radiotherapy (CFRT) in non-small cell lung cancer (NSCLC). Methods Lewis tumor-bearing mice were randomly divided into control, CFRT and SBHFRT groups. On the days 7 and 14 after radiation, the expression rates of CD4+, CD8+, Foxp3+, and CD86 + cells and levels of Ki-67 + protein were detected by immunohistochemical analysis, and the tumor necrosis rate was calculated. Following this, interleukin-10 (IL-10), IL-12, and interferon-γ (INF-γ) levels were detected by enzyme-linked immunosorbent assay. The apoptosis rate was evaluated via flow cytometry. The tumor volume and tumor growth inhibition rate (TGIR) were calculated on day 14. Tumor metabolism was assessed via micro 18F-FDG positron emission tomography/computer tomography. Results The tumor volume in the SBHFRT group reduced by 22.0% and TGIR increased by 92.2% (P < 0.05). Further, on days 7 and 14 after radiation, tumor metabolism, Ki-67 + and Foxp3 + expression rates, and IL-10 levels were lower and tumor necrosis and apoptosis rates; CD86+, CD4+, and CD8 + expression rates; and IL-12 and INF-γ levels were higher in SBHFRT group than in the CFRT group, particularly on day 7. Conclusion SBHFRT could lead to more accumulation of dendritic cells and anti-tumor lymphocytes and cytokines in the tumor tissue, and further reduce the aggregation of immunosuppressive lymphocytes and cytokines in the tumor tissue compared with CFRT, and the difference was the most obvious was day 7 after radiation. Hypofractionated radiotherapy combined with immunotherapy may be better for treating NSCLC, as observed on day 7 after radiation.
Comparative study of the rhizospheric arbuscular mycorrhizal fungi from three medicinal licorice plants from Xinjiang
Authors: Gao Tiantian , Wang Zhongke , Lv Xinhua , Li Guifang , He Yaling , Zhuang Li
Published: May 2020

We investigated the rhizospheric arbuscular mycorrhizal (AM) fungi from the three medicinal licorice plants, i.e., Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza glabra L. The AM fungi were reported to be affected by the host plant species as well as soil depth, physical, and chemical properties. We collected the rhizosphere from these licorice plants, from the soil layer with a depth of 0-20 cm, 20-40 cm, and 40-60 cm in the Xinjiang region. Furthermore, we employed the Illumina Miseq high-throughput sequencing platform to investigate the structure and diversity of these AM fungal communities. The soil physical and chemical properties were also evaluated to determine the relationship between the medicinal Glycyrrhiza rhizospheric AM fungi and the associated soil factors. In order to provide a reference for revealing the role of arbuscular mycorrhizal fungi in increasing the content of active components in cultivated licorice root, we isolated a total of 34 AM fungi that encompassed a phylum, a class, five (orders, families, and genus), and the Glomus and Paraglomus emerged as the dominant genus. We observed the highest diversity in the AM fungi in Glycyrrhiza uralensis Fisch. The Glycyrrhiza inflata showed the highest richness, whereas the Glycyrrhiza glabra showed the lowest richness and diversity in each soil layer. The plant species influenced the AM fungi more than the soil depth. The total phosphorus, available phosphorus, and organic matter in the soil prominently impacted the distribution of soil AM fungi. In contrast, the soil ammonium and nitrogen content had the lowest-impact on the AM fungi distribution. The genus Glomus was was found to be positively correlated with total phosphorus (P<0.001), and negatively correlated with total potassium (P<0.001), total salt (P<0.01), soil water content, and organic matter (P<0.05). The genus Paraglomus was found to be negatively correlated with the total phosphorus (P<0.001), whereas positively correlated with total potassium (P<0.001), total salt (P<0.001), organic matter (P<0.05), and fast-acting potassium (P<0.05). Besides, we found a positive correlation between the genus Diversispora and ammonium nitrogen (P<0.05), whereas the available phosphorus (P<0.05) and total salt (P<0.05) were negatively correlated with the genus Diversispora.
Biomechanical study on the stress distribution of the knee joint after distal femoral fracture malunion with residual varus-valgus deformity
Authors: Li Ming , Hu Pan , Ma Lijie , Zhang Di , Chang Wenli , Zhu Yanbin , Zhang Tao , Wei Ning , Chen Wei
Published: May 2020

Background

To investigate the effect of residual varus and valgus deformity on the stress distribution of knee joint after distal femoral fracture malunion.
Methods

Fourteen adult cadaver specimens with formalin were selected to establish the femoral fractures models, which were fixed subsequently at neutral position (anatomical reduction) and malunion positions (at 3 degrees, 7 degrees, 10 degrees valgus positions and 3 degrees, 7 degrees, and 10 degrees varus positions). The stress distribution on the medial and lateral plateau of the femur was quantitatively measured using ultra-low pressure sensitive film technology. The change of stress distribution of knee joint after femoral fracture malunion and the relationship between stress value and residual varus varus or valgus deformity were analyzed.
Results

Under 400 N vertical load, the stress values on the medial and lateral plateau of the femur at the neutral position were 1.162 ± 0.114 MPa and 1.103 ± 0.144 MPa, respectively. When compared with the stress values measured at the neutral position, the stress on the medial plateau of femur were significantly higher at varus deformities and lower at valgus deformities, and the stress on the lateral plateau was significantly higher at valgus deformity and lower at varus deformities (all P < 0.05). The stress values on the medial plateau of tibia were significantly higher than the corresponding data on the lateral plateau at neutral and 3 degrees, 7 degrees, 10 degrees varus deformities, respectively (all P < 0.05), and significantly lower than the corresponding data on the lateral plateau at 3 degrees, 7 degrees, 10 degrees valgus deformities, respectively (all P < 0.05).
Conclusions

Residual varus and valgus deformity after femoral fracture malunion can cause obvious changes of the stress distribution of knee joint. Therefore, the distal femoral fracture should be anatomically reduced and rigidly fixed to avoid residual varus-valgus deformity and malalignment of lower limbs.