bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of the eHealth Intervention ‘MyPlan 1.0’ on Physical Activity in Adults Who Visit General Practice: A Quasi-Experimental Trial
Authors: Degroote Laurent , Plaete Jolien , De Bourdeaudhuij Ilse , Verloigne Maïté , Van Stappen Vicky , De Meester An , Poppe Louise , Van der Mispel Celien , Crombez Geert
Published: January 2018

Physical inactivity is one of the major risk factors for poor health in the world. Therefore, effective interventions that promote physical activity are needed. Hence, we developed an eHealth intervention for adults, i.e., ‘MyPlan 1.0’, which includes self-regulation techniques for behaviour change. This study examined the effect of ‘MyPlan 1.0’ on physical activity (PA) levels in general practice. 615 adults (≥18 years) were recruited in 19 Flemish general practices, for the intervention group (n = 328) or for the wait-list control group (n = 183). Participants in the intervention group received the web-based intervention ‘MyPlan 1.0’ and were prompted to discuss their personal advice/action plan with their general practitioner. Participants in the wait-list control group only received general advice from the website. Self-reported physical activity was assessed with the International Physical Activity Questionnaire (IPAQ) at baseline and after one month. A three-level (general practice, adults, time) regression analysis was conducted in MLwiN. Significant intervention effects were found for total PA and moderate to vigorous PA with an increase for the intervention group compared to a decrease in the control condition. However, there was a high dropout rate in the intervention group (76%) and the wait-list control group (57%). Our self-regulation intervention was effective in increasing physical activity levels in adults. Future studies should consider strategies to prevent the large dropout from participants.
Small Molecules in Rare Tumors: Emerging Role of MicroRNAs in GIST
Authors: Kupcinskas Juozas
Published: January 2018

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of gastrointestinal tract. GISTs have very different clinical phenotypes and underlying molecular characteristics that are not yet completely understood. microRNAs (miRNAs) have been shown to participate in carcinogenesis pathways through post-transcriptional regulation of gene expression in different tumors. Over the last years emerging evidence has highlighted the role of miRNAs in GISTs. This review provides an overview of original research papers that analyze miRNA deregulation patterns, functional role, diagnostic, therapeutic and prognostic implications in GIST as well as provides directions for further research in the field.
Altered Circadian Timing System-Mediated Non-Dipping Pattern of Blood Pressure and Associated Cardiovascular Disorders in Metabolic and Kidney Diseases
Authors: Rahman Asadur , Hasan Arif , Nishiyama Akira , Kobori Hiroyuki
Published: January 2018

The morning surge in blood pressure (BP) coincides with increased cardiovascular (CV) events. This strongly suggests that an altered circadian rhythm of BP plays a crucial role in the development of CV disease (CVD). A disrupted circadian rhythm of BP, such as the non-dipping type of hypertension (i.e., absence of nocturnal BP decline), is frequently observed in metabolic disorders and chronic kidney disease (CKD). The circadian timing system, controlled by the central clock in the suprachiasmatic nucleus of the hypothalamus and/or by peripheral clocks in the heart, vasculature, and kidneys, modulates the 24 h oscillation of BP. However, little information is available regarding the molecular and cellular mechanisms of an altered circadian timing system-mediated disrupted dipping pattern of BP in metabolic disorders and CKD that can lead to the development of CV events. A more thorough understanding of this pathogenesis could provide novel therapeutic strategies for the management of CVD. This short review will address our and others’ recent findings on the molecular mechanisms that may affect the dipping pattern of BP in metabolic dysfunction and kidney disease and its association with CV disorders.
Differential Glycosylation and Modulation of Camel and Human HSP Isoforms in Response to Thermal and Hypoxic Stresses
Authors: Hoter Abdullah , Amiri Mahdi , Prince Abdelbary , Amer Hassan , Warda Mohamad , Naim Hassan
Published: January 2018

Increased expression of heat shock proteins (HSPs) following heat stress or other stress conditions is a common physiological response in almost all living organisms. Modification of cytosolic proteins including HSPs by O-GlcNAc has been shown to enhance their capabilities for counteracting lethal levels of cellular stress. Since HSPs are key players in stress resistance and protein homeostasis, we aimed to analyze their forms at the cellular and molecular level using camel and human HSPs as models for efficient and moderate thermotolerant mammals, respectively. In this study, we cloned the cDNA encoding two inducible HSP members, HSPA6 and CRYAB from both camel (Camelus dromedarius) and human in a Myc-tagged mammalian expression vector. Expression of these chaperones in COS-1 cells revealed protein bands of approximately 25-kDa for both camel and human CRYAB and 70-kDa for camel HSPA6 and its human homologue. While localization and trafficking of the camel and human HSPs revealed similar cytosolic localization, we could demonstrate altered glycan structure between camel and human HSPA6. Interestingly, the glycoform of camel HSPA6 was rapidly formed and stabilized under normal and stress culture conditions whereas human HSPA6 reacted differently under similar thermal and hypoxic stress conditions. Our data suggest that efficient glycosylation of camel HSPA6 is among the mechanisms that provide camelids with a superior capability for alleviating stressful environmental circumstances.
Regulatory Role of MicroRNAs in Muscle Atrophy during Exercise Intervention
Authors: Zhang Shufang , Chen Ning
Published: January 2018

Skeletal muscle comprising approximately 40% of body weight is highly important for locomotion and metabolic homeostasis. The growth and regeneration of skeletal muscle are highly organized processes; thus, it is not surprising to reveal certain complexity during these regulatory processes. Recently, a large number of evidence indicate that microRNAs can result in obvious impacts on growth, regeneration and metabolism of skeletal muscle. In this review, recent research achievements of microRNAs in regulating myogenesis, atrophy and aging during exercise intervention are discussed, which will provide the guidance for developing potential applications of microRNAs in health promotion and rehabilitation of sports injuries.
Inhibition of GLO1 in Glioblastoma Multiforme Increases DNA-AGEs, Stimulates RAGE Expression, and Inhibits Brain Tumor Growth in Orthotopic Mouse Models
Authors: Jandial Rahul , Neman Josh , Lim Punnajit , Tamae Daniel , Kowolik Claudia , Wuenschell Gerald , Shuck Sarah , Ciminera Alexandra , De Jesus Luis , Ouyang Ching , Chen Mike , Termini John
Published: January 2018

Cancers that exhibit the Warburg effect may elevate expression of glyoxylase 1 (GLO1) to detoxify the toxic glycolytic byproduct methylglyoxal (MG) and inhibit the formation of pro-apoptotic advanced glycation endproducts (AGEs). Inhibition of GLO1 in cancers that up-regulate glycolysis has been proposed as a therapeutic targeting strategy, but this approach has not been evaluated for glioblastoma multiforme (GBM), the most aggressive and difficult to treat malignancy of the brain. Elevated GLO1 expression in GBM was established in patient tumors and cell lines using bioinformatics tools and biochemical approaches. GLO1 inhibition in GBM cell lines and in an orthotopic xenograft GBM mouse model was examined using both small molecule and short hairpin RNA (shRNA) approaches. Inhibition of GLO1 with S-(p-bromobenzyl) glutathione dicyclopentyl ester (p-BrBzGSH(Cp)2) increased levels of the DNA-AGE N2-1-(carboxyethyl)-2′-deoxyguanosine (CEdG), a surrogate biomarker for nuclear MG exposure; substantially elevated expression of the immunoglobulin-like receptor for AGEs (RAGE); and induced apoptosis in GBM cell lines. Targeting GLO1 with shRNA similarly increased CEdG levels and RAGE expression, and was cytotoxic to glioma cells. Mice bearing orthotopic GBM xenografts treated systemically with p-BrBzGSH(Cp)2 exhibited tumor regression without significant off-target effects suggesting that GLO1 inhibition may have value in the therapeutic management of these drug-resistant tumors.
The Design and Development of Potent Small Molecules as Anticancer Agents Targeting EGFR TK and Tubulin Polymerization
Authors: Ihmaid Saleh , Ahmed Hany , Zayed Mohamed
Published: January 2018

Some novel anthranilate diamides derivatives 4a–e, 6a–c and 9a–d were designed and synthesized to be evaluated for their in vitro anticancer activity. Structures of all newly synthesized compounds were confirmed by infra-red (IR), high-resolution mass (HR-MS) spectra, 1H nuclear magnetic resonance (NMR) and 13C nuclear magnetic resonance (NMR) analyses. Cytotoxic screening was performed according to (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) assay method using erlotinib as a reference drug against two different types of breast cancer cells. The molecular docking study was performed for representative compounds against two targets, epidermal growth factor receptor (EGFR) and tubulin in colchicine binding site to assess their binding affinities in order to rationalize their anticancer activity in a qualitative way. The data obtained from the molecular modeling was correlated with that obtained from the biological screening. These data showed considerable anticancer activity for these newly synthesized compounds. Biological data for most of the anthranilate diamide showed excellent activity with nanomolar or sub nanomolar half maximal inhibitory concentration (IC50) values against tumor cells. EGFR tyrosine kinase (TK) inhibition assay, tubulin inhibition assay and apoptosis analysis were performed for selected compounds to get more details about their mechanism of action. Extensive structure activity relationship (SAR) analyses were also carried out.
The PUF Protein Family: Overview on PUF RNA Targets, Biological Functions, and Post Transcriptional Regulation
Authors: Wang Ming , Ogé Laurent , Perez-Garcia Maria-Dolores , Hamama Latifa , Sakr Soulaiman
Published: January 2018

Post-transcriptional regulation of gene expression plays a crucial role in many processes. In cells, it is mediated by diverse RNA-binding proteins. These proteins can influence mRNA stability, translation, and localization. The PUF protein family (Pumilio and FBF) is composed of RNA-binding proteins highly conserved among most eukaryotic organisms. Previous investigations indicated that they could be involved in many processes by binding corresponding motifs in the 3′UTR or by interacting with other proteins. To date, most of the investigations on PUF proteins have been focused on Caenorhabditis elegans, Drosophila melanogaster, and Saccharomyces cerevisiae, while only a few have been conducted on Arabidopsis thaliana. The present article provides an overview of the PUF protein family. It addresses their RNA-binding motifs, biological functions, and post-transcriptional control mechanisms in Caenorhabditis elegans, Drosophila melanogaster, Saccharomyces cerevisiae, and Arabidopsis thaliana. These items of knowledge open onto new investigations into the relevance of PUF proteins in specific plant developmental processes.
Prevalence of Vitamin D Deficiency Varies Widely by Season in Canadian Children and Adolescents with Sickle Cell Disease
Authors: Samson Kaitlyn , McCartney Heather , Vercauteren Suzanne , Wu John , Karakochuk Crystal
Published: January 2018

Sickle cell disease (SCD) is an inherited disorder caused by a variant (rs334) in the β-globin gene encoding hemoglobin. Individuals with SCD are thought to be at risk of vitamin D deficiency. Our aim was to assess serum 25-hydroxyvitamin D (25OHD) concentrations, estimate deficiency prevalence, and investigate factors associated with 25OHD concentrations in children and adolescents with SCD attending BC Children’s Hospital in Vancouver, Canada. We conducted a retrospective chart review of SCD patients (2–19 y) from 2012 to 2017. Data were available for n = 45 patients with n = 142 25OHD measurements assessed using a EUROIMMUN analyzer (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany). Additional data were recorded, including age, sex, and season of blood collection. Linear regression was used to measure associations between 25OHD concentration and predictor variables. Overall, mean ± SD 25OHD concentration was 79 ± 36 nmol/L; prevalence of low 25OHD concentrations (<30, <40, and <75 nmol/L) was 5%, 17% and 50%, respectively. Mean 25OHD concentrations measured during Jul–Sep were higher (28 (95% confidence interval CI: 16–40) nmol/L higher, P < 0.001) compared to Jan–Mar. Vitamin D deficiency rates varied widely by season: Based on 25OHD <30 nmol/L, prevalence was 0% in Oct–Dec and 6% in Jan–Mar; based on <40 nmol/L, prevalence was 0% in Oct–Dec and 26% in Jan–Mar.
Red Blood Cell Transfusion Need for Elective Primary Posterior Lumbar Fusion in A High-Volume Center for Spine Surgery
Authors: Ristagno Giuseppe , Beluffi Simonetta , Tanzi Dario , Belloli Federica , Carmagnini Paola , Croci Massimo , D’Aviri Giuseppe , Menasce Guido , Pastore Juan , Pellanda Armando , Pollini Alberto , Savoia Giorgio
Published: January 2018

(1) Background: This study evaluated the perioperative red blood cell (RBC) transfusion need and determined predictors for transfusion in patients undergoing elective primary lumbar posterior spine fusion in a high-volume center for spine surgery. (2) Methods: Data from all patients undergoing spine surgery between 1 January 2014 and 31 December 2016 were reviewed. Patients’ demographics and comorbidities, perioperative laboratory results, and operative time were analyzed in relation to RBC transfusion. Multivariate logistic regression analysis was performed to identify the predictors of transfusion. (3) Results: A total of 874 elective surgeries for primary spine fusion were performed over the three years. Only 54 cases (6%) required RBC transfusion. Compared to the non-transfused patients, transfused patients were mainly female (p = 0.0008), significantly older, with a higher ASA grade (p = 0.0002), and with lower pre-surgery hemoglobin (HB) level and hematocrit (p < 0.0001). In the multivariate logistic regression, a lower pre-surgery HB (OR (95% CI) 2.84 (2.11–3.82)), a higher ASA class (1.77 (1.03–3.05)) and a longer operative time (1.02 (1.01–1.02)) were independently associated with RBC transfusion. (4) Conclusions: In the instance of elective surgery for primary posterior lumbar fusion in a high-volume center for spine surgery, the need for RBC transfusion is low. Factors anticipating transfusion should be taken into consideration in the patient’s pre-surgery preparation.
Benzodiazepines II: Waking Up on Sedatives: Providing Optimal Care When Inheriting Benzodiazepine Prescriptions in Transfer Patients
Authors: Guina Jeffrey , Merrill Brian
Published: January 2018

This review discusses risks, benefits, and alternatives in patients already taking benzodiazepines when care transfers to a new clinician. Prescribers have the decision—sometimes mutually agreed-upon and sometimes unilateral—to continue, discontinue, or change treatment. This decision should be made based on evidence-based indications (conditions and timeframes), comorbidities, potential drug-drug interactions, and evidence of adverse effects, misuse, abuse, dependence, or diversion. We discuss management tools involved in continuation (e.g., monitoring symptoms, laboratory testing, prescribing contracts, state prescription databases, stages of change) and discontinuation (e.g., tapering, psychotherapeutic interventions, education, handouts, reassurance, medications to assist with discontinuation, and alternative treatments).
Moonlighting with WDR5: A Cellular Multitasker
Authors: Guarnaccia Alissa , Tansey William
Published: January 2018

WDR5 is a highly conserved WD40 repeat-containing protein that is essential for proper regulation of multiple cellular processes. WDR5 is best characterized as a core scaffolding component of histone methyltransferase complexes, but emerging evidence demonstrates that it does much more, ranging from expanded functions in the nucleus through to controlling the integrity of cell division. The purpose of this review is to describe the current molecular understandings of WDR5, discuss how it participates in diverse cellular processes, and highlight drug discovery efforts around WDR5 that may form the basis of new anti-cancer therapies.
N-(4-Bromobenzyl)-2-(5,6-dimethyl-1H-benzo[d]imid-azol-2-yl)benzeneamine
Authors: Dziełak Monika , Trzybiński Damian , Czerwińska Jolanta , Majchrzak Bartosz , Tudek Barbara , Woźniak Krzysztof , Mieczkowski Adam
Published: January 2018

N-(4-Bromobenzyl)-2-(5,6-dimethyl-1H-benzo[d]imidazol-2-yl)benzeneamine was obtained by condensation of N-(4-bromobenzyl)-3,1-benzoxazine-2,4-dione (N-(4-bromobenzyl)isatoic anhydride) with 4,5-dimethyl-1,2-phenylenediamine in refluxing acetic acid. This is a rare example of condensation of N-substituted 3,1-benzoxazine-2,4-dione with 1,2-phenylenediamine, which resulted in the formation of a benzimidazole derivative with a moderate yield. Crystallographic studies and initial biological screening were performed for the obtained product.
Acknowledgement to Reviewers of Medicines in 2017
Authors:   Medicines Editorial Office
Published: January 2018

Peer review is an essential part in the publication process, ensuring that Medicines maintains high quality standards for its published papers [...]
Development of an AVF Stenosis Assessment Tool for Hemodialysis Patients Using Robotic Ultrasound System
Authors: Du Yi-Chun , Shih Jheng-Bang , Wu Ming-Jui , Chiou Chung-Yi
Published: January 2018

With the aging population and lifestyle changes, the number of hemodialysis (HD) patients increases year by year. The arteriovenous fistula (AVF) is the gold standard vascular access used to access the blood for HD treatment. Since the status of stenosis affects HD efficiency, current clinical practices usually use a Doppler ultrasound imaging system to assess the parameters of the stenosis, such as the degree of stenosis (DOS). Unfortunately, this is a very time-consuming task. Furthermore, it is difficult to stably maintain the ultrasound probe for a prolonged period to give doctors clearer or reproducible images. In this study, a robotic ultrasound system (RUS) with ultrasound sequential imaging analysis was designed to evaluate the DOS of the AVF. The sequential imaging analysis was capable of image smoothing and vessel boundary detection. It enabled clinicians to mark the thickness of the plaque for further processing. Finally, the system was used to reconstruct 3D models of fistulas and calculated the DOS for clinical assessment. We also designed a pressure sensing module attached to the ultrasound probe to prevent the probe from coming loose, vibrating, and exerting abnormal pressure on the skin. In the phantom test, the results showed that the error of the DOS that was calculated by RUS was less than 3%. The results of clinical trials obtained from ten patients show that the error between the RUS and clinicians’ measurement was about 10% and had a highly linear correlation (R Square > 0.95). In addition, the reproducibility error was about 3% and could effectively save about 46% of the time during clinical examinations.
Statistical Investigation of the Mechanical and Geometrical Properties of Polysilicon Films through On-Chip Tests
Authors: Mirzazadeh Ramin , Ghisi Aldo , Mariani Stefano
Published: January 2018

In this work, we provide a numerical/experimental investigation of the micromechanics-induced scattered response of a polysilicon on-chip MEMS testing device, whose moving structure is constituted by a slender cantilever supporting a massive perforated plate. The geometry of the cantilever was specifically designed to emphasize the micromechanical effects, in compliance with the process constraints. To assess the effects of the variability of polysilicon morphology and of geometrical imperfections on the experimentally observed nonlinear sensor response, we adopt statistical Monte Carlo analyses resting on a coupled electromechanical finite element model of the device. For each analysis, the polysilicon morphology was digitally built through a Voronoi tessellation of the moving structure, whose geometry was in turn varied by sampling out of a uniform probability density function the value of the over-etch, considered as the main source of geometrical imperfections. The comparison between the statistics of numerical and experimental results is adopted to assess the relative significance of the uncertainties linked to variations in the micro-fabrication process, and the mechanical film properties due to the polysilicon morphology.
Process Evaluation and Costing of a Multifaceted Population-Wide Intervention to Reduce Salt Consumption in Fiji
Authors: Webster Jacqui , Pillay Arti , Suku Arleen , Gohil Paayal , Santos Joseph , Schultz Jimaima , Wate Jillian , Trieu Kathy , Hope Silvia , Snowdon Wendy , Moodie Marj , Jan Stephen , Bell Colin
Published: January 2018

This paper reports the process evaluation and costing of a national salt reduction intervention in Fiji. The population-wide intervention included engaging food industry to reduce salt in foods, strategic health communication and a hospital program. The evaluation showed a 1.4 g/day drop in salt intake from the 11.7 g/day at baseline; however, this was not statistically significant. To better understand intervention implementation, we collated data to assess intervention fidelity, reach, context and costs. Government and management changes affected intervention implementation, meaning fidelity was relatively low. There was no active mechanism for ensuring food companies adhered to the voluntary salt reduction targets. Communication activities had wide reach but most activities were one-off, meaning the overall dose was low and impact on behavior limited. Intervention costs were moderate (FJD $277,410 or $0.31 per person) but the strategy relied on multi-sector action which was not fully operationalised. The cyclone also delayed monitoring and likely impacted the results. However, 73% of people surveyed had heard about the campaign and salt reduction policies have been mainstreamed into government programs. Longer-term monitoring of salt intake is planned through future surveys and lessons from this process evaluation will be used to inform future strategies in the Pacific Islands and globally.
Preparation and Characterization of Graphene Oxide-Modified Sapium sebiferum Oil-Based Polyurethane Composites with Improved Thermal and Mechanical Properties
Authors: Wu Guiying , Xu Xiaoling , He Xin , Yan Yunjun
Published: January 2018

Bio-based polyurethane (PU) composites with superior thermal and mechanical properties have received wide attention. This is due to the recent rapid developments in the PU industry. In the work reported here, novel nano-composites with graphene oxide (GO)-modified Sapium sebiferum oil (SSO)-based PU has been synthesized via in situ polymerization. GO, prepared using the improved Hummers method from natural graphene (NG), and SSO-based polyol with a hydroxyl value of 211 mg KOH/g, prepared by lipase hydrolysis, were used as raw materials. The microstructures and properties of GO and the nano-composites were both characterized using Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and tensile tests. The results showed that GO with its nano-sheet structure possessed a significant number of oxygen-containing functional groups at the surface. The nano-composites containing 1 wt % GO in the PU matrix (PU1) exhibited excellent comprehensive properties. Compared with those for pure PU, the glass transition temperature (Tg) and initial decomposition temperature (IDT) of the PU1 were enhanced by 14.1 and 31.8 °C, respectively. In addition, the tensile strength and Young’s modulus of the PU1 were also improved by 126% and 102%, respectively, compared to the pure PU. The significant improvement in both the thermal stability and mechanical properties for PU/GO composites was attributed to the homogeneous dispersion and good compatibility of GO with the PU matrix. The improvement in the properties upon the addition of GO may be attributable to the strong interfacial interaction between the reinforcing agent and the PU matrix.
Modelling Water Stress in a Shiraz Vineyard Using Hyperspectral Imaging and Machine Learning
Authors: Loggenberg Kyle , Strever Albert , Greyling Berno , Poona Nitesh
Published: January 2018

The detection of water stress in vineyards plays an integral role in the sustainability of high-quality grapes and prevention of devastating crop loses. Hyperspectral remote sensing technologies combined with machine learning provides a practical means for modelling vineyard water stress. In this study, we applied two ensemble learners, i.e., random forest (RF) and extreme gradient boosting (XGBoost), for discriminating stressed and non-stressed Shiraz vines using terrestrial hyperspectral imaging. Additionally, we evaluated the utility of a spectral subset of wavebands, derived using RF mean decrease accuracy (MDA) and XGBoost gain. Our results show that both ensemble learners can effectively analyse the hyperspectral data. When using all wavebands (p = 176), RF produced a test accuracy of 83.3% (KHAT (kappa analysis) = 0.67), and XGBoost a test accuracy of 80.0% (KHAT = 0.6). Using the subset of wavebands (p = 18) produced slight increases in accuracy ranging from 1.7% to 5.5% for both RF and XGBoost. We further investigated the effect of smoothing the spectral data using the Savitzky-Golay filter. The results indicated that the Savitzky-Golay filter reduced model accuracies (ranging from 0.7% to 3.3%). The results demonstrate the feasibility of terrestrial hyperspectral imagery and machine learning to create a semi-automated framework for vineyard water stress modelling.
What is the Best Configuration of Wearable Sensors to Measure Spatiotemporal Gait Parameters in Children with Cerebral Palsy?
Authors: Carcreff Lena , Gerber Corinna , Paraschiv-Ionescu Anisoara , De Coulon Geraldo , Newman Christopher , Armand Stéphane , Aminian Kamiar
Published: January 2018

Wearable inertial devices have recently been used to evaluate spatiotemporal parameters of gait in daily life situations. Given the heterogeneity of gait patterns in children with cerebral palsy (CP), the sensor placement and analysis algorithm may influence the validity of the results. This study aimed at comparing the spatiotemporal measurement performances of three wearable configurations defined by different sensor positioning on the lower limbs: (1) shanks and thighs, (2) shanks, and (3) feet. The three configurations were selected based on their potential to be used in daily life for children with CP and typically developing (TD) controls. For each configuration, dedicated gait analysis algorithms were used to detect gait events and compute spatiotemporal parameters. Fifteen children with CP and 11 TD controls were included. Accuracy, precision, and agreement of the three configurations were determined in comparison with an optoelectronic system as a reference. The three configurations were comparable for the evaluation of TD children and children with a low level of disability (CP-GMFCS I) whereas the shank-and-thigh-based configuration was more robust regarding children with a higher level of disability (CP-GMFCS II–III).
Analysis of 3D Scan Measurement Distribution with Application to a Multi-Beam Lidar on a Rotating Platform
Authors: Morales Jesús , Plaza-Leiva Victoria , Mandow Anthony , Gomez-Ruiz Jose , Serón Javier , García-Cerezo Alfonso
Published: January 2018

Multi-beam lidar (MBL) rangefinders are becoming increasingly compact, light, and accessible 3D sensors, but they offer limited vertical resolution and field of view. The addition of a degree-of-freedom to build a rotating multi-beam lidar (RMBL) has the potential to become a common solution for affordable rapid full-3D high resolution scans. However, the overlapping of multiple-beams caused by rotation yields scanning patterns that are more complex than in rotating single beam lidar (RSBL). In this paper, we propose a simulation-based methodology to analyze 3D scanning patterns which is applied to investigate the scan measurement distribution produced by the RMBL configuration. With this purpose, novel contributions include: (i) the adaption of a recent spherical reformulation of Ripley’s K function to assess 3D sensor data distribution on a hollow sphere simulation; (ii) a comparison, both qualitative and quantitative, between scan patterns produced by an ideal RMBL based on a Velodyne VLP-16 (Puck) and those of other 3D scan alternatives (i.e., rotating 2D lidar and MBL); and (iii) a new RMBL implementation consisting of a portable tilting platform for VLP-16 scanners, which is presented as a case study for measurement distribution analysis as well as for the discussion of actual scans from representative environments. Results indicate that despite the particular sampling patterns given by a RMBL, its homogeneity even improves that of an equivalent RSBL.
Wireless Networks under a Backoff Attack: A Game Theoretical Perspective
Authors: Parras Juan , Zazo Santiago
Published: January 2018

We study a wireless sensor network using CSMA/CA in the MAC layer under a backoff attack: some of the sensors of the network are malicious and deviate from the defined contention mechanism. We use Bianchi’s network model to study the impact of the malicious sensors on the total network throughput, showing that it causes the throughput to be unfairly distributed among sensors. We model this conflict using game theory tools, where each sensor is a player. We obtain analytical solutions and propose an algorithm, based on Regret Matching, to learn the equilibrium of the game with an arbitrary number of players. Our approach is validated via simulations, showing that our theoretical predictions adjust to reality.
Defining Benchmarks for Restoration of Green Infrastructure: A Case Study Combining the Historical Range of Variability of Habitat and Species’ Requirements
Authors: Manton Michael , Angelstam Per
Published: January 2018

Animal husbandry in Europe that sustained once wide-spread semi-natural grasslands has been replaced by maximum sustained yield agriculture and forestry. This transformation coincides with declining populations of species dependent on semi-natural grasslands. A key task is therefore to define benchmarks for landscape restoration in terms of well-planned functional habitat networks, i.e., green infrastructure. Using a representative example of the European landscape gradient between agricultural and forest landscapes in southern Sweden as a case study, we analyzed the historic range of variability of the total area, quality, and size of grassland patches, and compared this to the requirements of focal grassland species. Spatial data covering the past two centuries indicated a 75–80% loss of total grassland area. Three factors affected the functionality of grasslands as green infrastructure. First, during the period 1927–1976, the loss of all grassland areas with high nature values was 41–59%. Second, as a measure of alteration, the number of semi-natural grassland types declined from 5 to 1. Third, to address habitat fragmentation, an analysis of changes in grassland patch size showed that patches sufficiently large to support local populations of complete focal grasslands species assemblages declined by 89–100%. The cumulative effect of loss, alteration, and fragmentation over the past two centuries indicates that the functionality of semi-natural grasslands has declined by at least 98%. However, this estimate does not consider land use changes before 1800, reduced connectivity, and altered biotic and abiotic processes in both semi-natural grasslands and the surrounding matrix. We stress the need to define the historic range of variability as a benchmark in relation to species’ requirements to maintain semi-natural grasslands as green infrastructure. Finally, integrated land management and governance that support multi-functionality of grasslands is needed.
Environmental Values (2-MEV) and Appreciation of Nature
Authors: Bogner Franz
Published: January 2018

When monitoring the long history of empirical instruments for environmental attitude measurement, the Two Major Environmental Value model (2-MEV) with its two higher order factors: Preservation (PRE) and Utilization (UTL) has repeatedly and independently been confirmed. PRE assesses preferences towards conservation of nature and the environment, whilst UTL measures preferences towards utilization/exploitation of nature. The latter, however, does not yet include the positive aspects of benefitting from the (enjoyable) use of nature. Consequently, besides the established 2-MEV-battery, additional items from an “Appreciation of Nature” (APR) scale were applied to an Irish sample of 289 secondary school students (age: M = 14.3 years). Responses to the altogether 30-item battery were applied on an oblique rotation by using the Promax procedure: UTL and PRE appeared as orthogonally related factors, APR correlated to PRE with 0.283. Based on loading scores, the item number for each subscale was further reduced to make the analysis more manageable in educational outreach sites; on those sites, where completing questionnaires may well be quite unpopular, they are very much needed for planning and fine-tuning educational programs. Therefore, extending the 2-MEV scale with an added scale for Appreciation may help: (i) to judge participants’ feedback for adjusting/balancing program contents better; and (ii) to promote appreciation as a successful strategy for shifting individuals away from their individual exploitative preferences.
Transition towards Sustainable Solutions: Product, Service, Technology, and Business Model
Authors: Nasiri Mina , Rantala Tero , Saunila Minna , Ukko Juhani , Rantanen Hannu
Published: January 2018

Nowadays, the horse industry can be considered as an important industry in European countries and has a major role in agricultural industry throughout the world. Although today the diversity of the horse-related companies provides new markets and business opportunities, there are also some sustainable issues which needs to be addressed. Therefore, this study contributes to this research gap by reviewing the concept of sustainability and existing approaches to find sustainable solutions for companies. These sustainable approaches can be applied to products, services and technologies as well as business models, such as the product-service-system (PSS), circular economy (CE) and industrial symbiosis (IS). Although there seems to be a growing understanding of sustainable approaches and their role in sustainable development, there is a lack of research at the empirical level regarding the types of sustainability approaches (i.e., technologies, services, products and business models) that evolve in specific industries. The empirical data in this research have been collected from a cross-section of Finnish horse industry operators to determine how willing companies are to exploit approaches to sustainable solutions, as well as what the existing sustainable solutions are in this industry. The response rate of this study is approximately 24 percent, including 139 received valid responses among the sample of 580 operators.
Examining the Relationships of Image and Attitude on Visit Intention to Korea among Tanzanian College Students: The Moderating Effect of Familiarity
Authors: Kim Sung-Bum , Kwon Ki-Joon
Published: January 2018

The aim of this study was to investigate country image and attitudes on Tanzanian tourists’ intention to visit Korea. It also aimed to examine the moderating effects of destination and product familiarities in the relationship between country image and attitudes on future visit intention. We utilized data collected from 350 college students in Tanzania and found that participants’ cognitive image of Korea significantly influenced their affective image, in turn affecting attitudes toward country, products, and cuisine. Attitudes toward country, products, and cuisine, in turn, had positive effects on visit intention. We also showed the moderating effects of destination and product familiarities among the interrelationships.
Announcing the 2018 Toxics Travel Award for Post-Doctoral Fellows
Authors: Bellinger David
Published: January 2018

This year we enjoyed a large number of very highly meritorious applications for our annual Toxics Travel Award.
CRISPR–Cas9 Genetic Analysis of Virus–Host Interactions
Authors: Gebre Makda , Nomburg Jason , Gewurz Benjamin
Published: January 2018

Clustered regularly interspaced short palindromic repeats (CRISPR) has greatly expanded the ability to genetically probe virus–host interactions. CRISPR systems enable focused or systematic, genomewide studies of nearly all aspects of a virus lifecycle. Combined with its relative ease of use and high reproducibility, CRISPR is becoming an essential tool in studies of the host factors important for viral pathogenesis. Here, we review the use of CRISPR–Cas9 for the loss-of-function analysis of host dependency factors. We focus on the use of CRISPR-pooled screens for the systematic identification of host dependency factors, particularly in Epstein–Barr virus-transformed B cells. We also discuss the use of CRISPR interference (CRISPRi) and gain-of-function CRISPR activation (CRISPRa) approaches to probe virus–host interactions. Finally, we comment on the future directions enabled by combinatorial CRISPR screens.
Calcitriol Inhibits HCV Infection via Blockade of Activation of PPAR and Interference with Endoplasmic Reticulum-Associated Degradation
Authors: Lin Yu-Min , Sun Hung-Yu , Chiu Wen-Tai , Su Hui-Chen , Chien Yu-Chieh , Chong Lee-Won , Chang Hung-Chuen , Bai Chyi-Huey , Young Kung-Chia , Tsao Chiung-Wen
Published: January 2018

Vitamin D has been identified as an innate anti-hepatitis C virus (HCV) agent but the possible mechanisms for this issue remain unclear. Here, we clarified the mechanisms of calcitriol-mediated inhibition of HCV infection. Calcitriol partially inhibited HCV infection, nitric oxide (NO) release and lipid accumulation in Huh7.5 human hepatoma cells via the activation of vitamin D receptor (VDR). When cells were pretreated with the activators of peroxisome proliferator-activated receptor (PPAR)-α (Wy14643) and -γ (Ly171883), the calcitriol-mediated HCV suppression was reversed. Otherwise, three individual stimulators of PPAR-α/β/γ blocked the activation of VDR. PPAR-β (linoleic acid) reversed the inhibition of NO release, whereas PPAR-γ (Ly171883) reversed the inhibitions of NO release and lipid accumulation in the presence of calcitriol. The calcitriol-mediated viral suppression, inhibition of NO release and activation of VDR were partially blocked by an inhibitor of endoplasmic reticulum-associated degradation (ERAD), kifunensine. Furthermore, calcitriol blocked the HCV-induced expressions of apolipoprotein J and 78 kDa glucose-regulated protein, which was restored by pretreatment of kifunensine. These results indicated that the calcitriol-mediated HCV suppression was associated with the activation of VDR, interference with ERAD process, as well as blockades of PPAR, lipid accumulation and nitrative stress.
Radiation and Anti-Cancer Vaccines: A Winning Combination
Authors: Cadena Alexandra , Cushman Taylor , Anderson Clark , Barsoumian Hampartsoum , Welsh James , Cortez Maria
Published: January 2018

The emerging combination of radiation therapy with vaccines is a promising new treatment plan in the fight against cancer. While many cancer vaccines such as MUC1, p53 CpG oligodeoxynucleotide, and SOX2 may be great candidates for antitumor vaccination, there still remain many investigations to be done into possible vaccine combinations. One fruitful partnership that has emerged are anti-tumor vaccines in combination with radiation. Radiation therapy was previously thought to be only a tool for directly or indirectly damaging DNA and therefore causing cancer cell death. Now, with much preclinical and clinical data, radiation has taken on the role of an in situ vaccine. With both cancer vaccines and radiation at our disposal, more and more studies are looking to combining vaccine types such as toll-like receptors, viral components, dendritic-cell-based, and subunit vaccines with radiation. While the outcomes of these combinatory efforts are promising, there is still much work to be covered. This review sheds light on the current state of affairs in cancer vaccines and how radiation will bring its story into the future.
Use of primary care data to predict those most vulnerable to cold weather: a case-crossover analysis
Authors: Tammes Peter , Sartini Claudio , Preston Ian , Hay Alastair D , Lasserson Daniel , Morris Richard W
Published: January 2018

Background

The National Institute for Health and Care Excellence (NICE) recommends that GPs use routinely available data to identify patients most at risk of death and ill health from living in cold homes.

Aim

To investigate whether sociodemographic characteristics, clinical factors, and house energy efficiency characteristics could predict cold-related mortality.

Design and setting

A case-crossover analysis was conducted on 34 777 patients aged ≥65 years from the Clinical Practice Research Datalink who died between April 2012 and March 2014. The average temperature of date of death and 3 days previously were calculated from Met Office data. The average 3-day temperature for the 28th day before/after date of death were calculated, and comparisons were made between these temperatures and those experienced around the date of death.

Method

Conditional logistic regression was applied to estimate the odds ratio (OR) of death associated with temperature and interactions between temperature and sociodemographic characteristics, clinical factors, and house energy efficiency characteristics, expressed as relative odds ratios (RORs).

Results

Lower 3-day temperature was associated with higher risk of death (OR 1.011 per 1°C fall; 95% CI = 1.007 to 1.015; P<0.001). No modifying effects were observed for sociodemographic characteristics, clinical factors, and house energy efficiency characteristics. Analysis of winter deaths for causes typically associated with excess winter mortality (N = 7710) showed some evidence of a weaker effect of lower 3-day temperature for females (ROR 0.980 per 1°C, 95% CI = 0.959 to 1.002, P = 0.082), and a stronger effect for patients living in northern England (ROR 1.040 per 1°C, 95% CI = 1.013 to 1.066, P = 0.002).

Conclusion

It is unlikely that GPs can identify older patients at highest risk of cold-related death using routinely available data, and NICE may need to refine its guidance.
Periodic structures on liquid-phase smectic A, nematic and isotropic free surfaces
Authors: Bagdinova Anna N , Demikhov Evgeny I , Borisenko Nataliya G , Tolokonnikov Sergei M , Mishakov Gennadii V , Sharkov Andrei V
Published: January 2018

The free boundary of smectic A (SmA), nematic and isotropic liquid phases were studied using a polarized optical microscope, an interferometric surface structure analyzer (ISSA), an atomic force microscope (AFM) and a scanning near-field optical microscope (SNOM). Images of the SmA phase free surface obtained by the polarized microscope and ISSA are in good correlation and show a well-known focal domain structure. The new periodic stripe structure was observed by scanning near-field optical microscopy on the surface of the smectic A, nematic and isotropic phases. The properties of this periodic structure are similar to the charged liquid helium surface and can be explained by nonlinear electrostatic instabilities previously described.
Symptomatic Bochdalek Hernia in Adult: a Case Report
Authors: Souza Luis Eduardo Almeida de , Anjos Karlla Lorena Dos Santos , Sousa Danielle Oliveira , Loureiro Gabriel De Jesus da Fonseca , Centeno Deborah Marques , Lima Yana Cardoso de , Cordeiro Rodrigo Da Silva , Lisboa Felipe Teixeira , Machado João Paulo Da Silva , Matos Karen Giovana Leal , Vieira Mauro Carvalho
Published: January 2018

Background: The congenital malformation of the posterolateral portion of the diaphragm is called the Bochdalek hernia, and was first described in 1948 by anatomy professor Bochdalek. The diagnosis in adults is extremely rare, being in the majority, an occasional finding in a chest x-ray.

Case: The authors report a Bochdalek Hernia case in an adult pacient, with complaints of dyspnea and epigastralgia refractory to pharmacological treatment. The diagnosis was confirmed after a chest CT scan.

Conclusion: Bochdalek hernias are extremely rare in adults, commonly diagnosed in childhood. The importance of recognition of its diagnosis is crucial, so it should be always among differential diagnoses in radiology, due to the range of complications that may affect the patients.
Electromagnetic Chirps from Neutron Star–Black Hole Mergers
Authors: Schnittman Jeremy D. , Canton Tito Dal , Camp Jordan , Tsang David , Kelly Bernard J.
Published: January 2018

We calculate the electromagnetic signal of a gamma-ray flare coming from the surface of a neutron star shortly before merger with a black hole companion. Using a new version of the Monte Carlo radiation transport code Pandurata that incorporates dynamic spacetimes, we integrate photon geodesics from the neutron star surface until they reach a distant observer or are captured by the black hole. The gamma-ray light curve is modulated by a number of relativistic effects, including Doppler beaming and gravitational lensing. Because the photons originate from the inspiraling neutron star, the light curve closely resembles the corresponding gravitational waveform: a chirp signal characterized by a steadily increasing frequency and amplitude. We propose to search for these electromagnetic chirps using matched filtering algorithms similar to those used in LIGO data analysis.
The Impact of Baryonic Physics on the Kinetic Sunyaev–Zel’dovich Effect
Authors: Park 박현 Hyunbae 배 , Alvarez Marcelo A. , Bond J. Richard
Published: January 2018

Poorly understood “baryonic physics” impacts our ability to predict the power spectrum of the kinetic Sunyaev–Zel’dovich (kSZ) effect. We study this in a sample high-resolution simulation of galaxy formation and feedback, Illustris. The high resolution of Illustris allows us to probe the kSZ power spectrum on multipoles

. Strong AGN feedback in Illustris nearly wipes out gas fluctuations at

and at late times, likely somewhat underpredicting the kSZ power generated at

. The post-reionization kSZ power spectrum for Illustris is well-fit by

over

 10,000, somewhat lower than most other reported values but consistent with the analysis of Shaw et al. Our analysis of the bias of free electrons reveals subtle effects associated with the multi-phase gas physics and stellar fractions that affect even linear scales. In particular, there are fewer electrons in biased galaxies, due to gas-cooling and star formation, and this leads to an electron bias of less than one, even at low wavenumbers. The combination of bias and electron fraction that determines the overall suppression is relatively constant,

, but more simulations are needed to see if this is Illustris-specific. By separating the kSZ power into different terms, we find that at least 6% (10%) of the signal at ℓ = 3000 (10,000) comes from non-Gaussian connected four-point density and velocity correlations,

, even without correcting for the Illustris simulation box-size. A challenge going forward will be accurately modeling long-wave velocity modes simultaneously with Illustris-like high resolution to capture the complexities of galaxy formation and its correlations with large-scale flows.