bluuebunny/crossref_metadata_2025 huggingface dataset:
Platelet SR-PSOX/CXCL16–CXCR6 Axis Influences Thrombotic Propensity and Prognosis in Coronary Artery Disease
Authors: Guan Tianyun , Emschermann Frederic , Schories Christoph , Groga-Bada Patrick , Martus Peter , Borst Oliver , Gawaz Meinrad , Geisler Tobias , Rath Dominik , Chatterjee Madhumita
Published: September 2022

Platelets express the transmembrane chemokine SR-PSOX/CXCL16, proteolytic cleavage of which generates the sCXCL16 soluble-(s) chemokine. The sCXCL16 engages CXCR6 on platelets to synergistically propagate degranulation, aggregation and thrombotic response. Currently, we have investigated the pro-thrombotic and prognostic association of platelet CXCL16–CXCR6 axis in CAD-(n = 240; CCS n = 62; ACS n = 178) patients. Platelet surface-associated-CXCL16 and CXCR6 surface expression ascertained by flow cytometry correlated significantly with platelet activation markers (CD62P denoting degranulation and PAC-1 binding denoting α2bβ3-integrin activation). Higher platelet CXCL16 surface association (1st quartile vs. 2nd–4th quartiles) corresponded to significantly elevated collagen-induced platelet aggregation assessed by whole blood impedance aggregometry. Platelet-CXCL16 and CXCR6 expression did not alter with dyslipidemia, triglyceride, total cholesterol, or LDL levels, but higher (>median) plasma HDL levels corresponded with decreased platelet-CXCL16 and CXCR6. Although platelet-CXCL16 and CXCR6 expression did not change significantly with or correlate with troponin I levels, they corresponded with higher Creatine Kinase-(CK) activity and progressively deteriorating left ventricular ejection fraction (LVEF) at admission. Elevated-(4th quartile) platelet-CXCL16 (p = 0.023) and CXCR6 (p = 0.030) measured at admission were significantly associated with a worse prognosis. However, after Cox-PH regression analysis, only platelet-CXCL16 was ascertained as an independent predictor for all-cause of mortality. Therefore, the platelet CXCL16–CXCR6 axis may influence thrombotic propensity and prognosis in CAD patients.
NSD2 as a Promising Target in Hematological Disorders
Authors: Azagra Alba , Cobaleda César
Published: September 2022

Alterations of the epigenetic machinery are critically involved in cancer development and maintenance; therefore, the proteins in charge of the generation of epigenetic modifications are being actively studied as potential targets for anticancer therapies. A very important and widespread epigenetic mark is the dimethylation of Histone 3 in Lysine 36 (H3K36me2). Until recently, it was considered as merely an intermediate towards the generation of the trimethylated form, but recent data support a more specific role in many aspects of genome regulation. H3K36 dimethylation is mainly carried out by proteins of the Nuclear SET Domain (NSD) family, among which NSD2 is one of the most relevant members with a key role in normal hematopoietic development. Consequently, NSD2 is frequently altered in several types of tumors—especially in hematological malignancies. Herein, we discuss the role of NSD2 in these pathological processes, and we review the most recent findings in the development of new compounds aimed against the oncogenic forms of this novel anticancer candidate.
New Insights into the Pivotal Role of the Amygdala in Inflammation-Related Depression and Anxiety Disorder
Authors: Hu Ping , Lu Ying , Pan Bing-Xing , Zhang Wen-Hua
Published: September 2022

Depression and anxiety disorders are the two most prevalent psychiatric diseases that affect hundreds of millions of individuals worldwide. Understanding the etiology and related mechanisms is of great importance and might yield new therapeutic strategies to treat these diseases effectively. During the past decades, a growing number of studies have pointed out the importance of the stress-induced inflammatory response in the amygdala, a kernel region for processing emotional stimuli, as a potentially critical contributor to the pathophysiology of depression and anxiety disorders. In this review, we first summarized the recent progress from both animal and human studies toward understanding the causal link between stress-induced inflammation and depression and anxiety disorders, with particular emphasis on findings showing the effect of inflammation on the functional changes in neurons in the amygdala, at levels ranging from molecular signaling, cellular function, synaptic plasticity, and the neural circuit to behavior, as well as their contributions to the pathology of inflammation-related depression and anxiety disorders. Finally, we concluded by discussing some of the difficulties surrounding the current research and propose some issues worth future study in this field.
Hyperbaric Oxygen Therapy Alleviates Social Behavior Dysfunction and Neuroinflammation in a Mouse Model for Autism Spectrum Disorders
Authors: Fischer Inbar , Shohat Sophie , Levy Gilad , Bar Ela , Trangle Sari Schokoroy , Efrati Shai , Barak Boaz
Published: September 2022

Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder (NDD) characterized by impaired social communication and repetitive behavior, among other symptoms. ASD is highly heritable, with SHANK3 being one of the high-risk genes for ASD. In recent years, knowledge has been growing regarding the neuroplasticity effect induced by hyperbaric oxygen therapy (HBOT) and its potential use for ASD. Here, we characterized the effect of HBOT on a mouse model for ASD with the human genetic condition of InsG3680 mutation in the Shank3 gene. As compared to placebo, HBOT improved social behavior and reduced neuroinflammation in the cortex of the InsG3680(+/+) mice. Specifically, HBOT induced upregulation of Insulin-like growth factor 1 (Igf1) expression levels and reduced the number of Iba1-positive cells in the mouse model for ASD compared to placebo control. Together, our research suggests that HBOT has the potential to improve the clinical outcome of ASD by ameliorating some of the core pathophysiological processes responsible for the development of the disorder.
Targeting the Maladaptive Effects of Binge Drinking on Circadian Gene Expression
Authors: Grigsby Kolter , Ledford Courtney , Batish Tanvi , Kanadibhotla Snigdha , Smith Delaney , Firsick Evan , Tran Alexander , Townsley Kayla , Reyes Kaylee-Abril Vasquez , LeBlanc Katherine , Ozburn Angela
Published: September 2022

Previous studies (1) support a role of circadian genes in regulating alcohol intake, and (2) reveal that harmful alcohol use alters circadian rhythms. However, there is minimal knowledge of the effects of chronic alcohol processes on rhythmic circadian gene expression across brain regions important for circadian biology and alcohol intake. Therefore, the present study sought to test the effects of chronic binge-like drinking on diurnal circadian gene expression patterns in the master circadian pacemaker (SCN), the ventral tegmental area (VTA), and the nucleus accumbens (NAc) in High Drinking in the Dark-1 (HDID-1) mice, a unique genetic risk model for drinking to intoxication. Consistent with earlier findings, we found that 8 weeks of binge-like drinking reduced the amplitude of several core circadian clock genes in the NAc and SCN, but not the VTA. To better inform the use of circadian-relevant pharmacotherapies in reducing harmful drinking and ameliorating alcohol’s effects on circadian gene expression, we tested whether the casein kinase-1 inhibitor, PF-67046, or the phosphodiesterase type-4 (an upstream regulator of circadian signalling) inhibitor, apremilast, would reduce binge-like intake and mitigate circadian gene suppression. PF-67046 did not reduce intake but did have circadian gene effects. In contrast, apremilast reduced drinking, but had no effect on circadian expression patterns.
Role of RNAIII in Resistance to Antibiotics and Antimicrobial Agents in Staphylococcus epidermidis Biofilms
Authors: Minich Andrej , Lišková Veronika , Kormanová Ľubica , Krahulec Ján , Šarkanová Júlia , Mikulášová Mária , Levarski Zdenko , Stuchlík Stanislav
Published: September 2022

Staphylococcus epidermidis is a known opportunistic pathogen and is one of the leading causes of chronic biofilm-associated infections. Biofilm formation is considered as a main strategy to resist antibiotic treatment and help bacteria escape from the human immune system. Understanding the complex mechanisms in biofilm formation can help find new ways to treat resistant strains and lower the prevalence of nosocomial infections. In order to examine the role of RNAIII regulated by the agr quorum sensing system and to what extent it influences biofilm resistance to antimicrobial agents, deletion mutant S. epidermidis RP62a-ΔRNAIII deficient in repressor domains with a re-maining functional hld gene was created. A deletion strain was used to examine the influence of oxacillin in combination with vanillin on biofilm resistance and cell survival was determined. Utilizing real-time qPCR, confocal laser scanning microscopy (CLSM), and crystal violet staining analyses, we found that the RNAIII-independent controlled phenol soluble modulins (PSMs) and RNAIII effector molecule have a significant role in biofilm resistance to antibiotics and phenolic compounds, and it protects the integrity of biofilms. Moreover, a combination of antibiotic and antimicrobial agents can induce methicillin-resistant S. epidermidis biofilm formation and can lead to exceedingly difficult medical treatment.
Somatic Mutational Landscape in Mexican Patients: CDH1 Mutations and chr20q13.33 Amplifications Are Associated with Diffuse-Type Gastric Adenocarcinoma
Authors: Cerrato-Izaguirre Dennis , Chirino Yolanda I. , Prada Diddier , Quezada-Maldonado Ericka Marel , Herrera Luis A , Hernández-Guerrero Angélica , Alonso-Larraga Juan Octavio , Herrera-Goepfert Roberto , Oñate-Ocaña Luis F. , Cantú-de-León David , Meneses-García Abelardo , Basurto-Lozada Patricia , Robles-Espinoza Carla Daniela , Camacho Javier , García-Cuellar Claudia M. , Sánchez-Pérez Yesennia
Published: September 2022

The Hispanic population, compared with other ethnic groups, presents a more aggressive gastric cancer phenotype with higher frequency of diffuse-type gastric adenocarcinoma (GA); this could be related to the mutational landscape of GA in these patients. Using whole-exome sequencing, we sought to present the mutational landscape of GA from 50 Mexican patients who were treated at The Instituto Nacional de Cancerología from 2019 to 2020. We performed a comprehensive statistical analysis to explore the relationship of the genomic variants and clinical data such as tumor histology and presence of signet-ring cell, H. pylori, and EBV. We describe a potentially different mutational landscape between diffuse and intestinal GA in Mexican patients. Patients with intestinal-type GA tended to present a higher frequency of NOTCH1 mutations, copy number gains in cytobands 13.14, 10q23.33, and 12q25.1, and copy number losses in cytobands 7p12, 14q24.2, and 11q13.1; whereas patients with diffuse-type GA tended to present a high frequency of CDH1 mutations and CNV gains in cytobands 20q13.33 and 22q11.21. This is the first description of a mutational landscape of GA in Mexican patients to better understand tumorigenesis in Hispanic patients and lay the groundwork for discovering potential biomarkers and therapeutic targets.
Fcα Receptor-1-Activated Monocytes Promote B Lymphocyte Migration and IgA Isotype Switching
Authors: Bos Amélie V. , van Gool Melissa M. J. , Breedveld Annelot C. , van der Mast Richard , Marsman Casper , Bouma Gerd , van de Wiel Mark A. , van Ham S. Marieke , Mebius Reina E. , van Egmond Marjolein
Published: September 2022

Patients with inflammatory bowel disease (IBD) produce enhanced immunoglobulin A (IgA) against the microbiota compared to healthy individuals, which has been correlated with disease severity. Since IgA complexes can potently activate myeloid cells via the IgA receptor FcαRI (CD89), excessive IgA production may contribute to IBD pathology. However, the cellular mechanisms that contribute to dysregulated IgA production in IBD are poorly understood. Here, we demonstrate that intestinal FcαRI-expressing myeloid cells (i.e., monocytes and neutrophils) are in close contact with B lymphocytes in the lamina propria of IBD patients. Furthermore, stimulation of FcαRI-on monocytes triggered production of cytokines and chemokines that regulate B-cell differentiation and migration, including interleukin-6 (IL6), interleukin-10 (IL10), tumour necrosis factor-α (TNFα), a proliferation-inducing ligand (APRIL), and chemokine ligand-20 (CCL20). In vitro, these cytokines promoted IgA isotype switching in human B cells. Moreover, when naïve B lymphocytes were cultured in vitro in the presence of FcαRI-stimulated monocytes, enhanced IgA isotype switching was observed compared to B cells that were cultured with non-stimulated monocytes. Taken together, FcαRI-activated monocytes produced a cocktail of cytokines, as well as chemokines, that stimulated IgA switching in B cells, and close contact between B cells and myeloid cells was observed in the colons of IBD patients. As such, we hypothesize that, in IBD, IgA complexes activate myeloid cells, which in turn can result in excessive IgA production, likely contributing to disease pathology. Interrupting this loop may, therefore, represent a novel therapeutic strategy.
Prediction of the Hydrodynamic Forces for a Ship Oscillating in Calm Water by an Improved Higher Order Rankine Panel Method
Authors: Wu Chun-Hsien , Fang Ming-Chung
Published: September 2022

This paper presents a frequency-domain Rankine source method based on a biquadratic B-spline scheme with an improved radiation mechanism. The improved radiation mechanism, based on the introduction of spatially varying Rayleigh artificial damping in addition to the simplified Seto’s radiation boundary conditions, is considered for modeling radiation of generated waves at various τ conditions, where τ=ωU/g including the undercritical condition (τ<0.25); this condition is present when a ship undergoes slow translation or low oscillatory frequency. In evaluations, the proposed method yields accurate solutions for unsteady flows produced by an oscillating, translationally moving submerged singularity. The radiation problem induced by a RIOS bulker is solved to have the resultant added mass and damping coefficients for further comparisons with the experimental data and the public numerical prediction by a simplified combined method at a wide τ region.
Systolic Arterial Pressure Control Using an Automated Closed-Loop System for Vasopressor Infusion during Intermediate-to-High-Risk Surgery: A Feasibility Study
Authors: Rinehart Joseph , Desebbe Olivier , Berna Antoine , Lam Isaac , Coeckelenbergh Sean , Cannesson Maxime , Joosten Alexandre
Published: September 2022

Introduction: Vasopressor infusions are essential in treating and preventing intraoperative hypotension. Closed-loop vasopressor therapy outperforms clinicians when the target is set at a mean arterial pressure (MAP) baseline, but little is known on the performance metrics of closed-loop vasopressor infusions when systolic arterial pressure (SAP) is the controlled variable. Methods: Patients undergoing intermediate- to high-risk abdominal surgery were included in this prospective cohort feasibility study. All patients received norepinephrine infusion through a computer controlled closed-loop system that targeted SAP at 130 mmHg. The primary objective was to determine the percent of case time in hypotension or under target defined as SAP below 10% of the target (SAP < 117 mmHg). Secondary objectives were the percent of case time “above target” (SAP > 10% of the target or >143 mmHg) and “in target” (within 10% of the SAP target or SAP between 117 and 143 mmHg). Results: A total of 12 patients were included. The closed-loop system infused norepinephrine for a median of 94.6% (25–75th percentile: 90.0–98.0%) of case time. The percentage of case time in hypotension or under target was only 1.8% (0.9–3.6%). The percentages of case time “above target” and “in target” were 4.7% (3.2–7.5%) and 92.4% (90.1–96.3%), respectively. Conclusions: This closed-loop vasopressor system minimizes intraoperative hypotension and maintains SAP within 10% of the target range for >90% of the case time in patients undergoing intermediate- to high-risk abdominal surgery.
Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering
Authors: Devi G.V. Yashaswini , Nagendra Apoorva H , Shenoy P. Sudheer , Chatterjee Kaushik , Venkatesan Jayachandran
Published: September 2022

Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects. Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute. The physiochemical characterization that includes FT-IR, XRD, and TGA confirms the structural integration between the materials. The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness. The incorporation of GO enhanced the mechanical strength of the Alg-HA-GO-F. The findings demonstrate the slower degradation and improved protein adsorption in the fucoidan-loaded scaffolds. The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage. The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold’s osteointegration and bone-bonding capability. In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control. Further, the results determined the augmented alkaline phosphatase and mineralization activity. The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.
Ocellatuperoxides A–F, Uncommon Anti-Tumoral γ-Pyrone Peroxides from a Photosynthetic Mollusk Placobranchus ocellatus
Authors: Li Song-Wei , Wu Qihao , Xu Heng , Yao Li-Gong , Luo Cheng , Wang Hong , Zhang Hao , Li Xu-Wen , Guo Yue-Wei
Published: September 2022

Six new pairs of γ-pyrone polypropionate enantiomers with an unusual peroxyl bridge at the side chain, namely (±)-ocellatuperoxides A–F (1–6), were isolated and characterized from the South China Sea photosynthetic mollusk Placobranchus ocellatus. Extensive spectroscopic analysis, single crystal X-ray diffraction analysis, ECD- (electronic circular dichroism) comparison, and TDDFT (time-dependent density functional theory) ECD computation were used to determine the structures and absolute configurations of new compounds. In a cell viability assay, several compounds showed considerable anti-tumoral effects on human non-small cell lung cancer cells A549 with Gefitinib (7.4 μM) and Erlotinib (2.1 μM) as positive controls. Further RNA-sequencing analysis and gene expression evaluation indicated that the anti-tumoral activity of the most effective compound 3 was associated with the regulation of several important genes, such as FGFR1 and HDAC5.
Impact Joining of Pure Copper C1100 and Aluminum Alloy A6061-T6 Plates at Edges
Authors: Yamashita Minoru , Iwatsuka Toru , Taguchi Haruchika , Nikawa Makoto
Published: September 2022

Joining of pure copper C1100 and aluminum alloy A6061-T6 plates of 5 mm thickness was investigated. The method was developed by one of the authors, in which the newly created surfaces of a pair of plates obtained by high-speed shear were immediately in contact with a sliding motion with a small overlap length. The total processing time was just about a few milliseconds. To create the new surface, high-speed shaving was also tested. The joining was not possible for the full thickness of the plates. A sharp notch was observed at the joint boundary due to a large shear droop in the copper. Shaving decreased the shear droop, and the joint length through the plate thickness became longer. The joining performance was evaluated by a uniaxial tensile test. The joint efficiency reached 100% using the specimen cut out from the really joined boundary. The affected zone of joining was confirmed by the hardness distribution near the boundary. It was about 30% of the thickness of the plate, which was much smaller than that in welding by heat, and no softened zone was found in both materials.
Eficacia de la garantía de acción de protección. Análisis de casos: cantón Ibarra, año 2019
Authors: Trujillo Tamayo Eliana Alejandra , López Ruiz Iliana , Aguirre Hernández Gabriela Patricia
Published: September 2022

La Constitución de la República del Ecuador del año 2008 prevé una serie de garantías jurisdiccionales destinadas a tutelares derechos constitucionales. El objetivo del presente artículo es determinar la eficacia de la Acción de protección, como mecanismo de tutela de derechos. Para ello se analizan casos en el cantón Ibarra durante el año 2019, utilizando algunos criterios como la duración de los procesos, decisión judicial en primera instancia entre otros, que permitirán determinar si la garantía es o no eficaz para los fines propuestos por el legislador. Al ser un estudio cualitativo el mismo permite examinar elementos jurídicos, doctrinarios y jurisprudenciales para finalmente establecer si es realmente eficaz o no esta garantía en su ámbito de aplicación. Se puede concluir que en la acción de protección se advierte la existencia de fallas en materia de celeridad procesal, lo que afecta el objeto y la naturaleza jurídica de esta garantía.
Bibliography of Utah Geology 1976
Authors:   Utah Geological and Mineral Survey
Published: September 2022

Papers on the geology and mineral resources of Utah that have appeared during 1976 are listed here along with 1974 and 1975 papers that did not appear in the two previous bibliographies.
Time for Revitalisation of Value Chain Management: A Reassessment of Porter’s View on Procurement
Authors: Dale Mark
Published: September 2022

Purpose: In the light of recent disruptive events, global supply chains have displayed their vulnerability, which has stressed the importance of procurement in modern practice. This study aims to analyse if the procurement function can be reconsidered as aprimary activity in Porter’s (1985) value chain.
Design/methodology/approach: The study utilises secondary data analysis. Data were collected on the basis of searches with nine keywords, which led to a discovery of 63 sources.
Findings: The study has found that the procurement function cannot be regarded as a primary activity. However, it is identified that the unification of fragments of the two activities, inbound logistics and procurement, creates the foundation for a new activity, which mitigates exposure to volatility. Consequently, the findings urge to incorporate procurement logistics into the value chain as a re-modified primary activity.
Иранские шлемы «кула-худ» в комплексе защитного вооружения тюркских кочевников Центральной Азии XVII–XIX вв = Iranskiye shlemy «kula-khud» v komplekse zashchitnogo vooruzheniya tyurkskikh kochevnikov Tsentral'noy Azii XVII–XIX vv
Authors: БОБРОВ Леонид
Published: September 2022

The article considers the topic of the evolution of Iranian „kula-hud” helmets, their construction and decorations system in the perspective of their employment by Turkic nomads of Central Asia. Basing on thorough analyze of the sources it was proved that the „kula-hud” helmet type was created on the foundation of the helmets manufactured from one-piece of metal from Near East in high Middle Ages. „Kula-hud” were originally formed by adding a moveable nasal, two sockets at the temples and a sharp spike at the top of hemispherical body. Chain mail aventail was also added. Early variants of the helmets of this type (proto-kula-hud) can be found as early as 16th century and should be linked with the reform of Iranian protective armament by the first Safavids. In 17th century „kula-hud” helmets were spread widely not only in Iran and India but also in Turkic states of Central Asia. An important factor in popularization of „kula-hud” was its relation with other weaponry (archery, sabers) and the tactics of the peoples of the area. The analyze of „kula-hud” helmets from Central Asia allowed to state that the majority of them are the imports from Iran. Part of the helmets was manufactured by the local masters according to Iranian patterns. The modification of some traditional riveted segmented helmets proves high prestige of Iranian armament among Turkic nomads of the area.
Innovative Technologies as a Factor in Improving the Quality of Laboratory Research
Authors: Выдрицкий А.В. , Бородовская В.Н. , Таврель А.А. , Белоусова Г.В. , Пархамович В.Н. , Муравская Я.П. , Гулякевич А.Г.
Published: September 2022

Введение. Современные инновационные технологии позволяют значительно повысить уровень качества оказания медицинской помощи населению, в том числе и в области лабораторных исследований.Цель. Изучение инновационных технологий, реализованных на всех этапах лабораторного анализа на примере ИООО «Независимая лаборатория ИНВИТРО».Материалы и методы. Анализ влияния внедренных инновационных технологий на различные показатели (экономические, технические, временные) в деятельности ИООО «Независимая лаборатория ИНВИТРО».Результаты. В результате проведенного исследования сделана оценка влияния инновационных технологий на качество предоставляемых лабораторных исследований.Выводы. Внедрение инновационных технологий, как разработанных в группе компаний ИНВИТРО, так и предлагаемых мировыми производителями, позволяет значительно повысить качество оказания лечебно-диагностической помощи населению.

Introduction. Modern innovative technologies can significantly increase the level of quality of medical care to the population, including in the field of laboratory research.Purpose. The study of innovative technologies implemented at all stages of laboratory analysis on the example of Independent Laboratory INVITRO.Materials and methods. Analysis of the impact of introduced innovative technologies on various indicators (economic, technical, temporary) in the activities of Independent Laboratory INVITRO.Results. As a result of the study, an assessment was made of the impact of innovative technologies on the quality of laboratory tests provided.Conclusions. The introduction of innovative technologies, both developed by the INVITRO group of companies and offered by global manufacturers, can significantly improve the quality of medical and diagnostic care for the population.
Evaluation of T- and B-cell Immunity Based on the T- and B-cell Receptor DNA Excision Circles (TREC/KREC) Detection Technology
Authors: Полякова Е.А. , Стёганцева М.В. , Луцкович Д.В. , Белевцев М.В.
Published: September 2022

Цель. Разработать эффективный молекулярно-биологический метод количественного определения кольцевых фрагментов ДНК Т- и В-клеточного рецептора в периферической крови для диагностики нарушений состояния клеточного иммунитета. Материалы и методы. Объектом исследования служили образцы периферической крови 20 здоровых детей в возрасте 9,0 (1,0–15,0) года и 30 детей с первичным и вторичным иммунодефицитом в возрасте 7,16 (0,1–18,0) года, контингент которых включал пациентов с диагнозами «первичный иммунодефицит» (n=21) и «вторичный иммунодефицит» после трансплантации гемопоэтических стволовых клеток (n=9). Результаты. Согласно полученным результатам, созданные калибраторы характеризовались высокой стабильностью и воспроизводимостью получаемых при их использовании результатов. В результате валидации разработанной технологии, в основе которой лежит полимеразная цепная реакция в реальном времени, установлено, что данная технология лабораторного исследования обладает высокой чувствительностью, специфичностью и эффективностью.Заключение. Разработанная технология позволяет выявить различные виды нарушений с вовлечением иммунного механизма, которые сопровождаются дефицитом Т- и В-клеточного звена иммунитета.

Purpose. Developaneffectivemolecular-biologicalmethodforquantitativedetermination of T- and B-cell receptor DNA circle fragments in peripheral blood to diagnose cellular immunity disorders.Materials and methods. Subjects of the study were peripheral blood samples of 20 healthy children aged 9.0 (1.0–15.0) years and 30 children with primary and secondary immunodeficiency aged 7.16 (0.1–18.0) years, whose contingent included patients diagnosed with primary immunodeficiency (n=21) and secondary immunodeficiency after hematopoietic stem cell transplantation (n=9).Results. According to the results obtained, the created calibrators were characterized by high stability and reproducibility of the results obtained when using them. As a result of the validation of the developed technology based on "real-time" polymerase chain reaction, it was found that this technology of laboratory examination has high sensitivity, specificity and efficiency.Conclusion. The developed technology makes it possible to identify various types of disorders with involvement of the immune mechanism, which are accompanied by deficiency of T- and B -cell immunity.
Changes in the Intestinal Microbiome in Primary Immunodeficiency: Review of Current Data
Authors: Скоповец Е.Я. , Белевцев М.В.
Published: September 2022

Первичные иммунодефициты – группа гетерогенных генетических заболеваний, приводящих к развитию как качественной (или функциональной) недостаточности, так и неконтролируемой активации пролиферации иммунокомпетентных клеток с формированием аутоиммунных, аутовоспалительных и аллергических заболеваний. Кишечная микробиота и ее метаболиты влияют на функции иммунной защиты и иммунный гомеостаз как локально, так и системно. Это подтверждается тем, что многие пациенты с первичными иммунодефицитами имеют осложнения в виде воспалительных заболеваний кишечника. Недавние исследования показали, что генетические дефекты, вызывающие первичные иммунодефициты, приводят к нарушениям механизмов, лежащих в основе гомеостаза кишечника, изменению иммунного надзора за кишечным барьером, повышению кишечной проницаемости и бактериальной транслокации. Многие инфекционные агенты при первичных иммунодефицитах усиливают колонизацию патобионтов, что приводит к повышенной восприимчивости организма и развитию вторичных инфекционных заболеваний. Коррекция дисбиотических состояний c помощью изменения состава микробиоты кишечника может быть полезна для снижения риска инфекционных осложнений заболевания.

Primary immunodeficiencies are a group of heterogeneous genetic diseases that lead to the development of both qualitative and (or) functional deficiency, as well as uncontrolled activationoftheproliferationofimmunocompetentcellsandtheformationofautoimmune, autoinflammatory and allergic diseases. The gut microbiota and its metabolites influence immune defense functions and immune homeostasis both locally and systemically. This is supported by the fact that many patients with primary immunodeficiencies have complications in the form of inflammatory bowel disease. Recent studies have shown that the genetic defects that cause primary immunodeficiencies lead to disruption of the mechanisms underlying intestinal homeostasis, altered immune surveillance of the intestinal barrier, increased intestinal permeability, and bacterial translocation. Many infectious agents in primary immunodeficiencies enhance the colonization of pathobionts, which leads to an increased susceptibility of the body and the development of secondary infectious diseases. Correction of dysbiotic conditions by changing the composition of the intestinal microbiota may be useful in reducing the risk of infectious complications of the disease.
Las guerras latinoamericanas del siglo XIX desde una perspectiva de género
Authors: Miseres Vanesa
Published: September 2022

Las guerras del siglo XIX en América Latina han perdurado en la memoria colectiva de la región hasta el presente. Los conflictos armados no sólo han definido la historia y la política del continente, sino que también han operado en el ámbito simbólico, condicionando las concepciones de género con mayor eficacia que cualquier otra práctica o institución de las naciones recién fundadas. La guerra actuó como una fuerza conservadora que organizó la sociedad en estructuras binarias que determinaban que mientras los hombres luchaban en el frente de batalla, las mujeres debían permanecer silenciosas y pacíficas en el frente doméstico (Higonnet 1993). En lo que William Acree (2013) ha llamado la “economía simbólica de la guerra,” las mujeres participaron en las luchas militares de sus países realizando tareas como la cocina, la costura o la enfermería. Estas actividades contribuyeron a la guerra y, al mismo tiempo, reforzaron entre los nuevos ciudadanos las nociones sobre la naturaleza doméstica del “sexo débil.”
Higienismo y maternidad: Carlos Costa y sus cartas “as senhoras brazileiras” (1877)
Authors: De Lara Portela Letícia , Barreto Amorim Pilla Maria Cecilia
Published: September 2022

El presente artículo tiene como objetivo investigar los consejos sobre el universo de la maternidad contenidos en las 24 cartas escritas por el reconocido médico carioca Carlos Antonio de Paula Costa, en 1877 para Gazeta de Notícias, diario de gran relevancia en Rio de Janeiro que introdujo una serie de innovaciones en la prensa de la época. De esa suerte, según las fuentes seleccionadas, se eligió la metodología de análisis del discurso a partir de las percepciones de Michel Foucault (1996, 1999), Sandra Pesavento (2012) y José D'Assunção Barros (2019), entendiendo el discurso como un método que nos permite analizar los agentes que crean valores compartidos en la sociedad. Así, buscamos entender, desde los preceptos higienistas, un discurso científico que predicaba que la maternidad era la principal función social de la mujer, encaminada a una misión civilizadora en Brasil. En ese sentido, la división de roles en la sociedad se vio a través de construcciones de género, según Joan Scott (1995) y Pierre Bourdieu (2020). Se pretendía, por lo tanto, analizar cómo la construcción social dirigió el asesoramiento médico a las mujeres, que como madres deben cuidar y amamantar a sus propios hijos e hijas.
Antifascismo y género en América Latina: el caso del Movimiento Pro Emancipación de las Mujeres de Chile (MEMCh) entre 1935 y 1939
Authors: Olivares Olivares Valeria Alejandra
Published: September 2022

El presente artículo analiza el caso del Movimiento Pro Emancipación de las Mujeres de Chile entre 1935 y 1939 y su autodefinición como organismo antifascista. A partir de los aportes de la historia del antifascismo y de la perspectiva de género, se estudia la manera en que sus integrantes forjaron relaciones con feministas antifascistas de Europa y Latinoamérica. Se propone que la influencia del antifascismo en las relaciones internacionales del MEMCh, dimensión poco explorada hasta la fecha, fue fundamental en su conformación identitaria y en el compromiso con problemáticas más allá de sus fronteras nacionales. Lo anterior, permite concluir que el MEMCh tuvo un rol fundamental en el fortalecimiento de la red antifascista internacional de mujeres en Chile.
Cultura universitária e experiência de juventude de estudantes de geografia da FCT/UNESP - Presidente Prudente
Authors: Turino Silva João Pedro
Published: September 2022

A pesquisa tem como foco a cultura universitária que se constitui no curso de Geografia da Faculdade de Ciências e Tecnologia, da Universidade Estadual Paulista “Júlio de Mesquita Filho” (FCT/UNESP), Presidente Prudente, mais particularmente a experiência que têm dela jovens estudantes do segundo e terceiro anos do curso (inicialmente, antes da pandemia de Covid 19, a ideia era trabalhar com o primeiro e segundo anos), justamente aqueles que estão passando pelo processo de inserção na vida universitária, vindos de trajetórias de vida e com projetos de futuro muito diversos. . As questões principais que orientaram a pesquisa foram: quem são os jovens que chegam ao curso de Geografia da FCT/UNESP e quais eram suas experiências prévias de juventude e que experiências passaram a ter quando ingressam no curso? O que seria a graduação em Geografia da FCT/UNESP enquanto contexto socioespacial e cultural específico, que tornaria esta experiência de juventude singular? Como os estudantes em sua diversidade articulam sua especificidade às experiências como estudantes de Geografia? Para responder estas questões, seguimos um percurso investigativo que articulou estratégias quantitativas e qualitativas de produção de informação.
Edição Completa
Authors: Mares Rizia Mendes
Published: September 2022

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In Mexico-Tenochtitlan ialtepetzintiliz: la fundación de la urbe mexica
Authors: Galindo Trejo Jesús
Published: September 2022

El ámbito celeste jugó un papel fundamental en el desarrollo de la cultura mesoamericana. Las principales deidades poseían una contraparte en el firmamento y eran objeto de un esmerado e intenso culto. A partir de fuentes etnohistóricas del siglo XVI, de una escultura de gran significado simbólico para los mexicas y de varios eventos astronómicos que sucedieron en el año de 1325, se propone una fecha específica de la fundación de Mexico-Tenochtitlan.
Interface de comunicación remota entre un sistema clasificador de ondas cerebrales y un robot móvil
Authors: Juarez-Moreno Iván , Marin-Urias Luis , Vazquez-Santacruz J. Alejandro , Vigueras-Zuñiga Marco
Published: September 2022

Teleoperation of robots allows humans to reach inaccessible places such as water depths greater than 20 m., near volcanoes, mines. Etc. Today this action is carried out by physical electronic controls, such as levers, however much training is needed by humans to control these devices due to the complexity of operation. To teleoperate robots in a more natural way arises the idea of developing a classifier brainwave system, based on statistical data, send generated commands intentions of human movement, thus is intended to provide a way to control the movements robots in different and more understandable without much training by the operator environments. The work presented proposes an entire framework to carry out a remote control of a mobile robot using a classifier brainwave, obtaining movement commands; this is done through free technologies making it a robust, reliable and future tool available to those who require it.
Sentido Artificial Ultrasónico
Authors: Perez Manuel , Donato José
Published: September 2022

Contribution: This project is very important for the educational program Career Industrial Maintenance, because it combines the knowledge of several materials such as Analog and Digital Electronics, Automation, Industrial Networking, Robotics and Programming among some others. Because of its low cost, this device is intended to be marketed or by any social program, distributed among all the people who need it to perform their daily activities as best as possible and actively integrate into productive society.
De la Lucha de Clases a la Lucha de humanidades
Authors: Méndez González Alejandro , Peralta Ávila Pablo Humberto
Published: September 2022

El presente artículo tiene el propósito de profundizar en la categoría “lucha de humanidades” propuesta por Luis Eduardo Primero Rivas, como una herramienta de análisis de la realidad ética y política. Esta herramienta teórico-metodológica va más allá del análisis socioeconómico de la lucha de clases al abordar todos los elementos que conforman el paradigma antropológico que implica cada modelo de humanidad. Este tipo de análisis se realiza desde una racionalidad analógica que evita enjuiciar cada modelo desde el estrado de la univocidad hegemónica, sino valorando las similitudes e interpretando las distinciones de cada modelo de humanidad en lucha por afirmar su identidad ante los paradigmas hegemónicos.
Conocimiento e interpretación: la nueva epistemología analógica.
Authors: Beuchot Puente Mauricio
Published: September 2022

Objetivo: Perfilar los rasgos de la nueva epistemología analógica. Metodología: Análisis teórico-conceptual. Resultados: La nueva epistemología surge de la hermenéutica analógica. Así, conjunta extremos y aun opuestos; se coloca entre la univocidad y la equivocidad, sacando lo mejor de cada una; reduce la dicotomía entre la teoría y la praxis, así como entre la razón y la emoción, enriqueciendo el conocimiento con perspectivas de ambos opuestos; abre el abanico de las interpretaciones, para enriquecer la interpretación, hacerla más fina y adecuada, más comprensiva y explicativa, pero sin renunciar a la verdad o la objetividad, que será equilibrada y situada, como es propio del analogismo. Conclusión: Entre el univocismo de los positivismos y el equivocismo de los posmodernismos, surge una salida: la epistemología analógica, que es mediadora entre esos extremos y ayuda dinamizar la actividad filosófica de América Latina con un pensamiento poscolonial analógico.
Peningkatan Kinerja Aparatur Sipil Negara Melalui Disiplin dan Kompetensi (Studi Kasus Sekretariat Daerah Kabupaten Jayawijaya)
Authors: Takdir Soltan , Ridwan Ridwan
Published: September 2022

Penelitian ini bertujuan menganalisis peningkatan kinerja Aparatur Sipil Negara melalui disiplin dan kompetensi. Penelitian ini menggunakan metode kuantitatif assosiatif dengan populasi penelitian sebanyak 75 Aparatur Sipil Negara Sekretariat Daerah Kabupaten Jayawijaya. Penentuan jumlah sampel menggunakan sampel jenuh dengan mengambil seluruh  Aparatur Sipil Negara Sekretariat Daerah Kabupaten Jayawijaya sebanyak 75 orang. Agar tujuan penelitian tercapai metode analisis data menggunakan analisis regresi linear berganda pada 3 variabel. Hasil dari penelitian ini menunjukkan Variabel Disiplin dan kompetensi pegawai berpengaruh positif dan signifikan terhadap peningkatan kinerja baik parsial maupun simultan dan memberikan persentase determinasi sebesar 36,3 % sedangkan sisanya 63,7% dipengaruhi oleh variabel lain diluar dari model analisis.This study aims to analyze the performance improvement of the State Civil Apparatus through discipline and competence. This study uses quantitative associative methods with a research population of 75 State Civil Apparatuses, Regional Secretariat of Jayawijaya Regency. Determination of the number of samples using a saturated sample by taking the entire State Civil Apparatus of the Regional Secretariat of Jayawijaya Regency as many as 75 people.  to achieve the research objectives, the data analysis method used multiple linear regression analysis on 3 variables. The results of this study indicate that the Discipline Variables and employee competencies have a positive and significant effect on increasing performance both partially and simultaneously and provide a percentage of determination of 36.3% while the remaining 63.7% is influenced by other variables outside of the analysis model.
Effect of Bile on Campylobacter species isolated from stool samples in Osogbo
Authors: Adekunle Olutoyin Catherine
Published: September 2022

Background: Campylobacter jejuni is a prevalent human pathogen and a major cause of bacterial gastroenteritis worldwide. In humans, C. jejuni colonises the intestinal tract, and its tolerance to bile is crucial for bacteria to survive and establish infection. Campylobacter jejuni and C. coli have the highest rate of foodborne-related clinical Campylobacteriosis. The study aims to determine the effect of bile salts, acid, and bacteriocin on campylobacter isolates obtained from stool samples.
Methods: Campylobacters were identified phenotypically in this study using biochemical tests and genotypically using 16S rRNA species-specific gene amplification by PCR. The confirmed twenty-five Campylobacter isolates comprising18 C. jejuni and 7 C. coli were tested for physiological factors such as bile tolerance, bacteriocin tolerance and ability to synthesise proteolytic enzymes on a solid medium.
Results: Campylobacter isolates survived at different concentrations of bile (2.1 -6.8%), low pH (7.1- 3.2) and in the presence of bacteriocin (3.8-6.8 AU/mL) with the production of proteolytic enzymes in the range of 16.2-15.2 mm.
Conclusion: The ability of Campylobacter spp to survive in the presence of bacteriocin and different concentrations of acid and bile salt indicates the strains’ virulence
Trichomoniasis among Pregnant Women Attending Ante-Natal Clinic in Eket, Akwa Ibom State, Nigeria
Authors: Udofia Lydia E. , Owowo Etanguno E.
Published: September 2022

Background: Trichomoniasis is a sexually transmitted disease detrimental to pregnant women. They are prone to vaginal pH increase during pregnancy, creating a conducive environment for the parasite. This study assessed the prevalence of Trichomonas vaginalis among pregnant women attending an ante-natal clinic in Immanuel General Hospital Eket, Akwa Ibom State.
Methods: Pre-designed questionnaires were administered to randomly selected pregnant women aged 18-49. High vaginal swabs were collected from each participant, and T. vaginalis was diagnosed using wet mount and Giemsa-stained microscopy method.
Results: Of the 200 samples collected, 28 (14%) were positive for Trichomonas vaginalis. The infection was highest among the age group 26-35 years, with a prevalence of 22 (78.57%). A higher prevalence of 16 (57.14%) was observed among women with elevated vaginal pH (>4.5) than those with lower pH (<4.5). The distribution of T. vaginalis by clinical manifestation revealed that all the positive women (28; 27.7%) had vaginal discharge and 11(14.3%) experienced itching. Few women experienced Dysuria (4;22.2%) and Dyspareunia (2; 10%). Chi-square analysis showed a significant difference in the age group (p< 0.05).
Conclusions: Routine screening of all pregnant women is recommended so that appropriate treatment can be administered early to avoid childbirth complications due to this disease. There is also a need for routine health talks on personal hygiene and safe sex practices.
EBUS-TBNA versus mediastinoscopy for mediastinal staging of lung cancer: a cost-minimization analysis
Authors: Pedro Steinhauser Motta1 João , Roberto Lapa e Silva1 José , Szklo1 Amir , E. Steffen2 Ricardo
Published: September 2022

Objective: To assess cost differences between EBUS-TBNA and mediastinoscopy for mediastinal staging of non-small cell lung cancer (NSCLC). Methods: This was an economic evaluation study with a cost-minimization analysis. We used a decision analysis software program to construct a decision tree model to compare the downstream costs of mediastinoscopy, EBUS-TBNA without surgical confirmation of negative results, and EBUS-TBNA with surgical confirmation of negative results for the mediastinal staging of NSCLC. The study was conducted from the perspective of the Brazilian public health care system. Only direct medical costs were considered. Results are shown in Brazilian currency (Real; R$) and in International Dollars (I$). Results: For the base-case analysis, initial evaluation with EBUS-TBNA without surgical confirmation of negative results was found to be the least costly strategy (R$1,254/I$2,961) in comparison with mediastinoscopy (R$3,255/I$7,688) and EBUS-TBNA with surgical confirmation of negative results (R$3,688/I$8,711). The sensitivity analyses also showed that EBUS-TBNA without surgical confirmation of negative results was the least costly strategy. Mediastinoscopy would become the least costly strategy if the costs for hospital supplies for EBUS-TBNA increased by more than 300%. EBUS-TBNA with surgical confirmation of negative results, in comparison with mediastinoscopy, will be less costly if the prevalence of mediastinal lymph node metastasis is = 38%. Conclusions: This study has demonstrated that EBUS-TBNA is the least costly strategy for invasive mediastinal staging of NSCLC in the Brazilian public health care system.