bluuebunny/crossref_metadata_2025 huggingface dataset:
Navigated intramedullary nailing for patients with intertrochanteric hip fractures is cost-effective at high-volume hospitals in mainland China: A markov decision analysis
Authors: Tang Liang , Yi Xiaoke , Yuan Ting , Li Hua , Xu Cheng
Published: January 2023

Objective

Previous studies have reported that navigation systems can improve clinical outcomes of intramedullary nailing (IMN) for patients with intertrochanteric fractures. However, information is lacking regarding the relationship between the costs of navigated systems and clinical outcomes. The present research aimed to evaluate the cost-effectiveness of navigated IMN as compared with traditional freehand IMN for patients with intertrochanteric fractures.

Methods

A Markov decision model with a 5-year time horizon was constructed to investigate the costs, clinical outcomes and incremental cost-effectiveness ratio (ICER) of navigated IMN for a 70-year-old patient with an intertrochanteric fracture in mainland China. The costs [Chinese Yuan (¥)], health utilities (quality-adjusted life-years, QALYs) and transition probabilities were obtained from published studies. The willingness-to-pay threshold for ICER was set at ¥1,40,000/QALY following the Chinese gross domestic product in 2020. Three institutional surgical volumes were used to determine the average navigation-related costs per patient: low volume (100 cases), medium volume (200 cases) and high volume (300 cases).

Results

Institutes at which 300, 200 and 100 cases of navigated IMN were performed per year showed an ICER of ¥43,149/QALY, ¥76,132.5/QALY and ¥1,75,083/QALY, respectively. Navigated IMN would achieve cost-effectiveness at institutes with an annual volume of more than 125 cases.

Conclusions

Our analysis demonstrated that the navigated IMN could be cost-effective for patients with inter-trochanteric fracture as compared to traditional freehand IMN. However, the cost-effectiveness was more likely to be achieved at institutes with a higher surgical volume.
Outcomes of integrated surgical wound treatment mode based on tibial transverse transport for diabetic foot wound
Authors: Chang Shusen , Zhang Fang , Chen Wei , Zhou Jian , Nie Kaiyu , Deng Chengliang , Wei Zairong
Published: January 2023

Background

Diabetic foot ulcer (DFU) is frequently difficult to heal and finally leads to amputation, resulting in high mortality rate in diabetic patients. To date, effective and optimal therapies are still lacking. This study aims to investigate the efficacy of integrated surgical wound treatment (ISWT) mode on diabetic foot wound.

Methods

From January 2021 to December 2021, 13 diabetic foot patients with Wagner grade 3 to 4 were treated with ISWT mode, which combined TTT technique with debridement, induced membrane technique, vacuum sealing drainage (VSD) technique and skin grafting technique. The time of wound healing, the skin temperature at midpoint of dorsum of affected foot (T), visual analogue scale (VAS) score and ankle-brachial index (ABI) was measured before and after surgery. CTA examination of the lower extremity arteries was performed at the end of the cortex transport to evaluate the small arteriolar formation of the lower extremity. The complications occurred in each patient were recorded.

Results

13 patients with age ranging from 45 to 66 years were followed up for 3 to 13 months. All patients healed completely without amputation being performed, no serious complications were found except for one case of nail channel infection. The mean healing time was 25.8 ± 7.8 days, with a range of 17 to 39 days. The mean time of carrying external fixation scaffolds and resuming walking was 71.8 ± 10.0 and 30.8 ± 9.1 days, with a range of 56 to 91 days and 18 to 45 days, respectively. The skin temperature at midpoint of dorsum of affected foot (T), VAS and ABI was all improved significantly at 3 months after surgery. Furthermore, CTA examination showed an increase in the number of lower extremity arteries and a thickening in the size of small arteriolar compared with those of pre-operative, and the collateral circulation of lower extremity was established and interweaved into a network.

Conclusion

Integrated surgical treatment of diabetic foot wound can achieve satisfactory clinical results.
Risk factors, prognostic potency, and longitudinal variation of anxiety and depression in postoperative glioma patients
Authors: Wu Xiaohua , Wang Dongdong , Wang Dan
Published: January 2023

Objective

Anxiety and depression are common mental disorders in glioma patients. This study aimed to evaluate the risk factors, prognostic role, and longitudinal changes in anxiety and depression in postoperative glioma patients.

Methods

Anxiety and depression were assessed by Hospital Anxiety and Depression Scale at baseline, month (M) 6, M12, M24 and M36 in 270 glioma patients after surgical resection. Furthermore, comprehensive clinic characteristics and treatment-related information were collected.

Results

Gender (female vs. male) (P = 0.014, odds ratio (OR) = 1.974), marital status (single/divorced/widowed vs. married) (P = 0.019, OR = 2.172), Karnofsky performance status (KPS) score (≤70 vs. > 70) (P = 0.002, OR = 2.556), World Health Organization (WHO) classification (high-grade glioma (HGG) vs. low-grade glioma (LGG)) (P = 0.005, OR = 2.155), and postoperative complications (yes vs. not) (P = 0.001, OR = 2.525) were independently related to anxiety occurrence. Marital status (single/divorced/widowed vs. married) (P = 0.034, OR = 2.026), KPS score (≤70 vs. > 70) (P < 0.001, OR = 3.880), WHO classification (HGG vs. LGG) (P = 0.032, OR = 1.810), and postoperative complications (yes vs. not) (P = 0.001, OR = 2.602) were independently related to depression occurrence. Besides, anxiety (P = 0.038) and depression (P = 0.013) were linked with shorter overall survival (OS), and depression was an independent risk factor for worse OS (P = 0.040, hazard ratio = 1.596). More importantly, anxiety and depression remained at a high prevalence during a 3-year follow-up.

Conclusion

Gender, marital status, KPS score, WHO classification, and postoperative complications are risk factors for anxiety and depression; moreover, anxiety and depression are at high prevalence continuously and correlated with worse survival in postoperative glioma patients.
Functional outcome of arterial thoracic outlet syndrome treatment
Authors: de Kleijn Robert J. C. M. F. , Schropp Ludo , Westerink Jan , van Hattum Eline S. , Petri Bart-Jeroen , de Borst Gert J.
Published: January 2023

Introduction

The low prevalence of Arterial Thoracic Outlet Syndrome (ATOS) and diffuse symptomatology have resulted in limited data on optimal treatment strategies and long-term outcome. The aim of this study was to report and evaluate a single center experience with the treatment of ATOS including midterm patient reported outcome.

Methods

All patients treated for ATOS from 2004 to 2020 were retrospectively identified. Patients were divided into two groups based on presenting symptoms; ATOX group (Acute arterial occlusion with ischemia) and ATOS group (claudication symptoms). Baseline characteristics and treatment details were extracted from electronic patient files. A telephone survey was conducted to collect patients' follow-up data including a functional disability score using the Quick Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire. The primary endpoint of this study was symptom-free survival. Secondary endpoints were median QuickDASH scores during follow-up, postoperative complications and possible re-interventions.

Results

A total of 20 patients (mean age 44.6 years, median follow-up 50.5 months) were included and divided into two groups (ATOXN = 9, ATOSN = 11). In the ATOX group, eight patients were primarily treated with catheter directed thrombolysis (CDT;N = 5) or surgical thrombectomy (N = 3). All patients received staged thoracic outlet decompression surgery (TOD). In the ATOS group, 10 patients primarily received TOD and one patient was treated conservatively with physiotherapy. Seven ATOX patients and nine ATOS patients were symptom free at follow-up with a median QuickDASH score of 2.3 (IQR 12.5) and 2.3 (IQR 16.5) respectively. Ten complications occurred in the ATOX group; three bleeding complications, five re-occlusions, one arterial dissection and one occipital infarction. In the ATOS group five complications occurred; one perioperative bleeding complication, three re-occlusions and a stent fracture. Seven vs. five re-interventions were required in the ATOX and ATOS groups respectively.

Conclusion

The mid-term self-reported symptom free survival in both the ATOX as well as ATOS group seems acceptable while median QuickDASH scores in both groups indicate a very good functional outcome. This however comes at the cost of treatment related bleeding complications in especially the ATOX group presumably due to thrombolysis, and re-interventions required in almost one out of three patients.
Trends in COVID-Related Activity in Sentinel Family Medicine Practices: An Observational Study
Authors: Maeder Muriel , Auderset Diane , Borel Bernard , Masserey Eric , Schwarz Joëlle , Mueller Yolanda
Published: January 2023

Objectives: During the COVID pandemic, data collected in family medicine were scarce. The COVID-FM project aimed to monitor trends of COVID-related activity in family medicine practices of the canton of Vaud, Switzerland, during the year 2021.

Methods: Practitioners were invited to join an ad hoc sentinel surveillance system. Online data collection was based on daily activity reports and monthly questionnaires. Participants categorized daily counts of consultations and phone calls into predefined categories. Data were reported and discussed on a weekly basis with public health authorities.

Results: On the target of 50 physicians, 37 general physicians from 32 practices finally constituted the COVID-FM sentinel network, contributing to 901 practice-weeks of surveillance in family medicine and 604 in paediatrics. In paediatrics, COVID-related activity corresponded mostly to COVID-19 diagnostic consultations (2911/25990 face-to-face consultations = 11.2%) while in family medicine, other COVID-related topics—such as questions on vaccination—predominated (4143/42221 = 9.8%).

Conclusion: COVID-related consultations constituted an important part of primary care practices’ activity in 2021. Monitoring COVID-related activity in primary care provided health authorities with valuable information to guide public health action.
Associations of Long-Term Physical Activity Trajectories With All-Cause Mortality in a General Population
Authors: Lee Chia-Lin , Liu Wei-Ju , Chen Ching-Hsien , Wang Jun-Sing
Published: January 2023

Objectives: We investigated the associations of mean levels of leisure-time physical activity (LTPA) and latent LTPA trajectories with all-cause mortality risk.

Methods: Trajectories of LTPA were established using group-based trajectory analysis with a latent class growth model in a population-based cohort between 1996 and 2014. A Cox-proportional hazard model was conducted to examine the associations of LTPA quintiles and LTPA trajectories with all-cause mortality.

Results: A total of 21,211 participants (age 18–90 years) were analyzed (median follow-up 16.8 years). The study participants were divided into five groups according to percentiles of LTPA (<20th, 20th–<40th, 40th–<60th, 60th–<80th, ≥80th) and LTPA trajectories (low/stable, medium/stable, increasing, decreasing, and fluctuating), respectively. Participants with a decreasing trajectory did not have a significantly lower risk of all-cause mortality despite having the highest baseline level of LTPA. In contrast, participants with a medium/stable (HR 0.84, 95% CI 0.72–0.98, p = 0.031) or an increasing (HR 0.57, 95% CI 0.33–0.97, p = 0.037) trajectory had a significantly lower risk of all-cause mortality.

Conclusion: Promotion of maintaining stable LTPA is beneficial for public health and survival.
Techno-Economic and Environmental Analysis of Decommissioned Flowline, Umbilical, and Tubular for Breakwaters
Authors: Zhang Xihong , Biswas Wahidul K. , Watt Andy , Philip Lendyn , Sadler Shaun
Published: January 2023

This paper presents the application of recycled tubular, flowline, and umbilical in coastal protection structures. Flowline and tubular are found to improve the load resistance capacity of concrete beams. Embedment of flowline, umbilical, and tubular into concrete beams would be beneficial to the structural performance, which do not noticeably alter the initial cracking strength of the concrete beam but will provide good post-cracking resistance. A techno-economic analysis was performed, which revealed that coastal protection concrete structures with decommissioned components accounting for more than 25% of the concrete weight could be both economically viable and environmentally friendly options. Since global warming is the most dominant environmental impact (i.e., 63%), recycling these decommissioned components from offshore structures could impose positive environmental impacts. Given the limited supply of construction materials in the remote coastal area as well as its proximity to decommissioned oil and gas rig sites, these decommissioned components could have great potential for use as construction materials in the coastal areas adjacent to the oil exploration. This preliminary study finds no showstopper for the concept of recycling the mentioned decommissioned components as coastal protection concrete structures from structural performance, environmental impact, and economic perspectives.
Characterization of Mortar from Casa Barbot (Portugal), a Case Study from the Beginning of the 20th Century
Authors: Maljaee Hamid , Silva António Santos , Velosa Ana
Published: January 2023

The popularity of natural cement (Roman cement) in buildings from the late 19th and the beginning of the 20th century and the high diversity of characteristics of natural cement demand research of mortar and binders used in the buildings of this period in Portugal. Understanding the type of binder used in the historic buildings will help the project/intervention team to make the best decisions in terms of the development of compatible mortars for historical conservation purposes. Casa Barbot is one of the Art Nouveau examples of the beginning of the 20th century in Portugal. The garden of this building is characterized by peculiar elements in terms of materials and architecture. Full characterization of the mortars employed in the construction of this building as well as identification of the used binder is the main objective of this study. The study comprises a wide range of laboratory characterization techniques such as X-ray fluorescence (XRF), X-ray diffraction (XRD), petrography, open porosity, density, water absorption by capillarity, and compressive strength. The results show the diversity of compositional characteristics in the mortars used in the decorative elements in the period garden of Casa Barbot. All mortars are composed of Portland cement with a mix of additives such as blast furnace slag, limestone filler, air lime, and the occasional presence of charcoal. The results raise doubt about the use of natural cement as stated in the historical documents. This study will broaden the scientific knowledge of the materials of that period in Portugal to provide a comprehensive plan for the preservation of historical buildings.
Introducing Concrete Fabrication into Ferrocement: A Study on the Shape-Making of Cement Mixture
Authors: Chen Fei , Pang Sibo , Yu Wanhong
Published: January 2023

Widely used in the making of sculptures and prefabricated building components, ferrocement is considered to be beneficial to environmental preservation. However, there is still little attention paid to the making shape and design of the ferrocement in the literature. Moreover, there are some disadvantages associated with ferrocement, such as the lengthy and complex process of binding poles and nets together. The research question to solve in this paper is about how to study the making shape of ferrocement when solving these disadvantages, so as to improve the characteristics of ferrocement. In this research, a dialogue is conducted between concrete fabrication and ferrocement, the focus of which is placed more on the material and craft instead of robotics as the recent frontier of concrete fabrication. By replacing the standard wire mesh in the ferrocement with steel, how to make the steel plate from two dimensions to three dimensions, and then assemble them into a steel skeleton is explored. Then, the craft of casting is studied for integration of the cement mixture into the steel skeleton and its tight attachment onto the steel surface with spraying. Apart from that, a digital software is applied to the simulation and design accompanied by physical experimentation. To sum up, the research demonstrates the potential of free-forming of the ferrocement and its application in the (prefabricated) building technology, with questions raised for future study.
Effects of Pounding and Abutment Behavior on Seismic Response of Multi-Span Bridge Considering Abutment-Soil-Foundation-Structure Interactions
Authors: Deng Yulin , Ge Shuxun , Lei Fan
Published: January 2023

This study aims to analyze the longitudinal seismic performance of a typical multi-span continuous girder bridge with seat-type abutments under earthquake excitation, especially accounting for different abutment behaviors. Three-dimensional finite element models of typical multi-span bridges are built considering the nonlinearity of the bridge columns, bearings, abutment-backfill interactions, pile-soil interactions, and the pounding at expansion joints. One of the models adopts a simplified bilinear model to express the force-displacement relationship of the abutment backwall. The other adopts a more practical multi-linear model, and the abutment backwall is used as a sacrificial component to control the damage to the abutment’s foundation by changing the strength of the abutment backwall. Comparisons of the results of the analysis of two bridge models with and without a sacrificial backwall indicate that it is more favorable for bridges with a sacrificial backwall to protect the foundation, but it is likely to arouse a larger displacement response of the main beam and even cause the unseating of girders. The recommendation for a sacrificial abutment in seismic design is that the right yield strength of the backwall should be selected to reach the balance point of force and displacement, and a collapse-proof system could be employed to prevent the beam from unseating.
Prospective Multicentric Assessment of 68Ga-DOTANOC PET/CT in Grade 1-2 GEP-NET
Authors: Lugat Alexandre , Frampas Éric , Touchefeu Yann , Mirallié Éric , Bras Maëlle Le , Senellart Hélène , Rauscher Aurore , Fleury Vincent , Campion Loïc , Rohmer Vincent , Couturier Olivier-François , Lebtahi Rachida , Rouzet François , Ruszniewski Philippe , Kraeber-Bodéré Françoise , Bourgeois Mickaël , Ansquer Catherine
Published: January 2023

The aim of this multicentric study was to prospectively compare 68Ga-DOTANOC PET/CT versus somatostatin receptor scintigraphy (SRS) with SPECT/CT, combined with multiphasic CT scan and MRI in patients with grade 1 or 2 gastroenteropancreatic neuroendocrine tumors (GEP-NET). Patients with histologically proven grade 1 or 2 GEP-NET with suspicion of recurrence or progression, or with typical aspects of GEP-NET on morphological imaging, were explored with conventional imaging (CI): SRS with SPECT/CT, multiphasic CT scan and/or liver MRI followed by 68Ga-DOTANOC PET/CT. The gold standard was based on histology and imaging follow-up. The data of 105 patients (45 woman and 60 men; median age) were analyzed. 68Ga-DOTANOC PET/CT sensitivity was significantly higher than CI sensitivity in per-patient (98.9% vs. 88.6%, p = 0.016) and per-region (97.6% vs. 75.6%, p < 0.001) analyses, in the detection of the primary (97.9% vs. 78.7%; p = 0.016), peritoneal carcinomatosis (95% vs. 30%, p < 0.001), and bone metastases (100% vs. 33.3%, p = 0.041). 68Ga-DOTANOC PET/CT had an impact on the therapeutic management of 41.9% (44/105) patients compared to decisions based on CI explorations. Our data confirm the superiority of 68Ga-DOTANOC PET/CT over CI in the detection of peritoneal carcinomatosis and bone metastasis, as well as its strong therapeutic impact on the management of patients with grade 1-2 GEP-NETs.
Multiphysics Modeling of Low-Intensity Pulsed Ultrasound Induced Chemotherapeutic Drug Release from the Surface of Gold Nanoparticles
Authors: Hornsby Tyler K. , Kashkooli Farshad Moradi , Jakhmola Anshuman , Kolios Michael C. , Tavakkoli Jahangir (Jahan)
Published: January 2023

Currently, no numerical model for low-intensity pulsed ultrasound (LIPUS)-triggered anticancer drug release from gold nanoparticle (GNP) drug carriers exists in the literature. In this work, LIPUS-induced doxorubicin (DOX) release from GNPs was achieved in an ex vivo tissue model. Transmission electronic microscopy (TEM) imaging was performed before and after LIPUS exposure, and significant aggregation of the GNPs was observed upon DOX release. Subsequently, GNP surface potential was determined before and after LIPUS-induced DOX release, using a Zetasizer. A numerical model was then created to predict GNP aggregation, and the subsequent DOX release, via combining a thermal field simulation by solving the bioheat transfer equation (in COMSOL) and the Derjaguin, Landau, Verwey, and Overbeek (DLVO) total interaction potential (in MATLAB). The DLVO model was applied to the colloidal DOX-loaded GNPs by summing the attractive van der Waals and electrostatic repulsion interaction potentials for any given GNP pair. DLVO total interaction potential was found before and after LIPUS exposure, and an energy barrier for aggregation was determined. The DLVO interaction potential peak amplitude was found to drop from 1.36 kBT to 0.24 kBT after LIPUS exposure, translating to an 82.4% decrease in peak amplitude value. It was concluded that the interaction potential energy threshold for GNP aggregation (and, as a result, DOX release) was equal to 0.24 kBT.
Metabolic Adjustments following Glutaminase Inhibition by CB-839 in Glioblastoma Cell Lines
Authors: De los Santos-Jiménez Juan , Rosales Tracy , Ko Bookyung , Campos-Sandoval José A. , Alonso Francisco J. , Márquez Javier , DeBerardinis Ralph J. , Matés José M.
Published: January 2023

Most tumor cells can use glutamine (Gln) for energy generation and biosynthetic purposes. Glutaminases (GAs) convert Gln into glutamate and ammonium. In humans, GAs are encoded by two genes: GLS and GLS2. In glioblastoma, GLS is commonly overexpressed and considered pro-oncogenic. We studied the metabolic effects of inhibiting GLS activity in T98G, LN229, and U87MG human glioblastoma cell lines by using the inhibitor CB-839. We performed metabolomics and isotope tracing experiments using U-13C-labeled Gln, as well as 15N-labeled Gln in the amide group, to determine the metabolic fates of Gln carbon and nitrogen atoms. In the presence of the inhibitor, the results showed an accumulation of Gln and lower levels of tricarboxylic acid cycle intermediates, and aspartate, along with a decreased oxidative labeling and diminished reductive carboxylation-related labeling of these metabolites. Additionally, CB-839 treatment caused decreased levels of metabolites from pyrimidine biosynthesis and an accumulation of intermediate metabolites in the de novo purine nucleotide biosynthesis pathway. The levels of some acetylated and methylated metabolites were significantly increased, including acetyl-carnitine, trimethyl-lysine, and 5-methylcytosine. In conclusion, we analyzed the metabolic landscape caused by the GLS inhibition of CB-839 in human glioma cells, which might lead to the future development of new combination therapies with CB-839.
Hepatitis Virus and Hepatocellular Carcinoma: Recent Advances
Authors: Shen Chen , Jiang Xin , Li Mei , Luo Yao
Published: January 2023

Hepatocellular carcinoma (HCC) remains a global health challenge, causing 600,000 deaths each year. Infectious factors, including hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis D virus (HDV), have long been considered the major risk factors for the development and progression of HCC. These pathogens induce hepatocyte transformation through a variety of mechanisms, including insertional mutations caused by viral gene integration, epigenetic changes, and the induction of long-term immune dysfunction. The discovery of these mechanisms, while advancing our understanding of the disease, also provides targets for new diagnostic and therapeutic approaches. In addition, the discovery and research of chronic HEV infection over the past decade indicate that this common hepatitis virus also seems to have the potential to induce HCC. In this review, we provide an overview of recent studies on the link between hepatitis virus and HCC, as well as new diagnostic and therapeutic approaches to HCC based on these findings. Finally, we also discuss the potential relationship between HEV and HCC. In conclusion, these associations will further optimize the diagnosis and treatment of infection-associated HCC and call for better management policies.
Configuring Therapeutic Aspects of Immune Checkpoints in Lung Cancer
Authors: Khadela Avinash , Chavda Vivek P. , Postwala Humzah , Ephraim Ramya , Apostolopoulos Vasso , Shah Yesha
Published: January 2023

Immune checkpoints are unique components of the body’s defense mechanism that safeguard the body from immune responses that are potent enough to harm healthy body cells. When proteins present on the surface of T cells recognize and bind to the proteins present on other tumor cells, immune checkpoints are triggered. These proteins are called immunological checkpoints. The T cells receive an on/off signal when the checkpoints interact with companion proteins. This might avert the host’s immune system from eliminating cancer cells. The standard care plan for the treatment of non-small cell lung cancer (NSCLC) has been revolutionized with the use of drugs targeting immune checkpoints, in particular programmed cell death protein 1. These drugs are now extended for their potential to manage SCLC. However, it is acknowledged that these drugs have specific immune related adverse effects. Herein, we discuss the use of immune checkpoint inhibitors in patients with NSCLC and SCLC, their outcomes, and future perspectives.
Integrated Photocatalytic Oxidation and Adsorption Approach for the Robust Treatment of Refinery Wastewater Using Hybrid TiO2/AC
Authors: Ul Haq Ihtisham , Ahmad Waqas , Ahmad Imtiaz , Shah Amjad , Yaseen Muhammad , Muhammad Taj
Published: January 2023

This study reports the removal of hydrocarbon (HC) pollutants from petroleum refinery wastewater by integrated photocatalytic oxidation and adsorption using a TiO2/AC hybrid material. The hybrid adsorbent/catalyst was prepared by the impregnation of TiO2 over AC and characterized by FTIR, SEM, EDX, and XRD analyses. Under the optimized reaction conditions of pH 3, 30 °C, and 1000 mg TiO2/AC per 500 mL of sample in 50 min, the integrated photocatalytic oxidation-adsorption achieved a net percentage removal of benzene, toluene, aniline, and naphthalene of 91% from model HC solutions. Under these conditions, for the treatment of real refinery wastewater, TiO2/AC caused a 95% decrease in chemical oxygen demand (COD). The integrated photocatalytic oxidation and adsorption using TiO2/AC showed a clear advantage over the individual adsorption and photocatalytic oxidation using AC and TiO2, whereby about the same level of removal of model HCs and a decrease in the COD of refinery wastewater was attained in 105 min and 90 min, respectively, utilizing larger adsorbent/catalyst dosages. GC-MS analysis revealed that during the integrated process of adsorption-photocatalytic oxidation, all the parent HCs and oxidation byproducts were completely removed from the refinery wastewater. Based on the outstanding performance, cost-effectiveness, and environmental greenness, the newly designed TiO2/AC via the integrated adsorption-photocatalytic oxidation can be counted as an effective alternative route for the large-scale processing of refinery wastewater.
A Review of the Single-Step Flame Synthesis of Defective and Heterostructured TiO2 Nanoparticles for Photocatalytic Applications
Authors: Khan Sovann , Park Jin-Sung , Ishihara Tatsumi
Published: January 2023

Titanium dioxide (TiO2) is an excellent UV-photocatalytic material that is widely used in various applications, including clean energy production, environmental remediation, and chemical production. However, the use of TiO2 is limited in the field of visible light photocatalysis due to its large bandgap and fast recombination rate between electron and hole pairs, which generally results in a low photocatalytic reaction. Defect/bandgap engineering by doping and the introduction of heterojunctions has been successfully employed to improve the photocatalytic activities of TiO2 over a wide wavelength. To apply the unconventional structured TiO2 with high photocatalytic performance to industries, the development of efficient methods for large-scale production is of high importance. Flame synthesis is a very promising method for the rapid production of nanoparticles. In this article, we summarize the latest reports on the synthesis of defective and heterostructured TiO2 using the single-step method of flame synthesis. Fundamental understandings of reactor configurations, synthesis conditions, precursor preparation and their physicochemical properties are intensively discussed.
Anticancer Asparaginases: Perspectives in Using Filamentous Fungi as Cell Factories
Authors: Garcia Pedro Henrique Dias , Costa-Silva Tales Alexandre , Gómez Martí Morera , Contesini Fabiano Jares , Canella Paula Renata Bueno Campos , Carvalho Patrícia de Oliveira
Published: January 2023

The enzyme L-asparaginase (L-asparagine amidohydrolase) catalyzes the breakdown of L-asparagine into aspartate and ammonia, which leads to an anti-neoplastic activity stemming from its capacity to deplete L-asparagine concentrations in the bloodstream, and it is therefore used in cases of acute lymphoblastic leukemia (ALL) to inhibit malignant cell growth. Nowadays, this anti-cancer enzyme, largely produced by Escherichia coli, is well established on the market. However, E. coli L-asparaginase therapy has side effects such as anaphylaxis, coagulation abnormality, low plasma half-life, hepatotoxicity, pancreatitis, protease action, hyperglycemia, and cerebral dysfunction. This review provides a perspective on the use of filamentous fungi as alternative cell factories for L-asparaginase production. Filamentous fungi, such as various Aspergillus species, have superior protein secretion capacity compared to yeast and bacteria and studies show their potential for the future production of proteins with humanized N-linked glycans. This article explores the past and present applications of this important enzyme and discusses the prospects for using filamentous fungi to produce safe eukaryotic asparaginases with high production yields.
Novel Highly Efficient Green and Reusable Cu(II)/Chitosan-Based Catalysts for the Sonogashira, Buchwald, Aldol, and Dipolar Cycloaddition Reactions
Authors: Dysin Artem P. , Egorov Anton R. , Khubiev Omar , Golubev Roman , Kirichuk Anatoly A. , Khrustalev Victor N. , Lobanov Nikolai N. , Rubanik Vasili V. , Tskhovrebov Alexander G. , Kritchenkov Andreii S.
Published: January 2023

In this study, new Cu(II)/chitosan-based systems were designed via (i) the treatment of chitosan with sodium sulfate (1a) or sodium acetate (1b); (ii) the coating of 1a or 2a with a sodium hyaluronate layer (2a and 2b, correspondingly); (iii) the treatment of a cholesterol–chitosan conjugate with sodium sulfate (3a) or sodium acetate (3b); and (iv) the succination of 1a and 1b to afford 4a and 4b or the succination of 2a and 2b to yield 5a and 5b. The catalytic properties of the elaborated systems in various organic transformations were evaluated. The use of copper sulfate as the source of Cu2+ ions results in the formation of nanoparticles, while the use of copper acetate leads to the generation of conventional coarse-grained powder. Cholesterol-containing systems have proven to be highly efficient catalysts for the cross-coupling reactions of different types (e.g., Sonogashira, Buchwald–Hartwig, and Chan–Lam types); succinated systems coated with a layer of hyaluronic acid are promising catalysts for the aldol reaction; systems containing inorganic copper(II) salt nanoparticles are capable of catalyzing the nitrile-oxide-to-nitrile 1,3-dipolar cycloaddition. The elaborated catalytic systems efficiently catalyze the aforementioned reactions in the greenest solvent available, i.e., water, and the processes could be conducted in air. The studied catalytic reactions proceed selectively, and the isolation of the product does not require column chromatography. The product is separated from the catalyst by simple filtration or centrifugation.
MHC Class II Presentation in Autoimmunity
Authors: Ishina Irina A. , Zakharova Maria Y. , Kurbatskaia Inna N. , Mamedov Azad E. , Belogurov Alexey A. , Gabibov Alexander G.
Published: January 2023

Antigen presentation by major histocompatibility complex class II (MHC-II) molecules is crucial for eliciting an efficient immune response by CD4+ T cells and maintaining self-antigen tolerance. Some MHC-II alleles are known to be positively or negatively associated with the risk of the development of different autoimmune diseases (ADs), including those characterized by the emergence of autoreactive T cells. Apparently, the MHC-II presentation of self-antigens contributes to the autoimmune T cell response, initiated through a breakdown of central tolerance to self-antigens in the thymus. The appearance of autoreactive T cell might be the result of (i) the unusual interaction between T cell receptors (TCRs) and self-antigens presented on MHC-II; (ii) the posttranslational modifications (PTMs) of self-antigens; (iii) direct loading of the self-antigen to classical MHC-II without additional nonclassical MHC assistance; (iv) the proinflammatory environment effect on MHC-II expression and antigen presentation; and (v) molecular mimicry between foreign and self-antigens. The peculiarities of the processes involved in the MHC-II-mediated presentation may have crucial importance in the elucidation of the mechanisms of triggering and developing ADs as well as for clarification on the protective effect of MHC-II alleles that are negatively associated with ADs.
PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
Authors: Ali Md Khadem , Tian Xuefei , Zhao Lan , Schimmel Katharina , Rhodes Christopher J. , Wilkins Martin R. , Nicolls Mark R. , Spiekerkoetter Edda F.
Published: January 2023

Bone morphogenic protein receptor 2 (BMPR2) expression and signaling are impaired in pulmonary arterial hypertension (PAH). How BMPR2 signaling is decreased in PAH is poorly understood. Protein tyrosine phosphatases (PTPs) play important roles in vascular remodeling in PAH. To identify whether PTPs modify BMPR2 signaling, we used a siRNA-mediated high-throughput screening of 22,124 murine genes in mouse myoblastoma reporter cells using ID1 expression as readout for BMPR2 signaling. We further experimentally validated the top hit, PTPN1 (PTP1B), in healthy human pulmonary arterial endothelial cells (PAECs) either silenced by siRNA or exposed to hypoxia and confirmed its relevance to PAH by measuring PTPN1 levels in blood and PAECs collected from PAH patients. We identified PTPN1 as a novel regulator of BMPR2 signaling in PAECs, which is downregulated in the blood of PAH patients, and documented that downregulation of PTPN1 is linked to endothelial dysfunction in PAECs. These findings point to a potential involvement for PTPN1 in PAH and will aid in our understanding of the molecular mechanisms involved in the disease.
Induced Remodelling of Astrocytes In Vitro and In Vivo by Manipulation of Astrocytic RhoA Activity
Authors: Domingos Cátia , Müller Franziska E. , Passlick Stefan , Wachten Dagmar , Ponimaskin Evgeni , Schwarz Martin K. , Schoch Susanne , Zeug André , Henneberger Christian
Published: January 2023

Structural changes of astrocytes and their perisynaptic processes occur in response to various physiological and pathophysiological stimuli. They are thought to profoundly affect synaptic signalling and neuron-astrocyte communication. Understanding the causal relationship between astrocyte morphology changes and their functional consequences requires experimental tools to selectively manipulate astrocyte morphology. Previous studies indicate that RhoA-related signalling can play a major role in controlling astrocyte morphology, but the direct effect of increased RhoA activity has not been documented in vitro and in vivo. Therefore, we established a viral approach to manipulate astrocytic RhoA activity. We tested if and how overexpression of wild-type RhoA, of a constitutively active RhoA mutant (RhoA-CA), and of a dominant-negative RhoA variant changes the morphology of cultured astrocytes. We found that astrocytic expression of RhoA-CA induced robust cytoskeletal changes and a withdrawal of processes in cultured astrocytes. In contrast, overexpression of other RhoA variants led to more variable changes of astrocyte morphology. These induced morphology changes were reproduced in astrocytes of the hippocampus in vivo. Importantly, astrocytic overexpression of RhoA-CA did not alter the branching pattern of larger GFAP-positive processes of astrocytes. This indicates that a prolonged increase of astrocytic RhoA activity leads to a distinct morphological phenotype in vitro and in vivo, which is characterized by an isolated reduction of fine peripheral astrocyte processes in vivo. At the same time, we identified a promising experimental approach for investigating the functional consequences of astrocyte morphology changes.
Application of Fluctuating Asymmetry Values in Pelophylax ridibundus (Amphibia: Anura: Ranidae) Meristic Traits as a Method for Assessing Environmental Quality of Areas with Different Degrees of Urbanization
Authors: Zhelev Zhivko , Mollov Ivelin , Tsonev Stefan
Published: January 2023

In this paper, we assess the environmental impact of urbanization in three freshwater biotopes, using the levels of fluctuating asymmetry (FA) in 10 meristic morphological traits in the Marsh Frog (Pelophylax ridibundus (Pallas, 1771)). Two of the studied biotopes are located in the boundaries of the city of Plovdiv (one in the central part, the other in a suburban residential area), and the third is located in the vicinity of the village of Orizare. Our working hypothesis is based on the assumption that urban and suburban sites are more severely affected by human activities than rural sites. However, according to our results, the population of P. ridibundus inhabiting Maritsa River in the central part of Plovdiv City, and that in the suburban zone, have found relatively good living conditions. Contrary to our expectations, the worst environmental conditions were observed in the rural zone, where anthropogenic stress related to intensive pastoral animal husbandry and crop farming was present. The absence of adult individuals in the rural site is also an indicator of unfavorable living conditions.
Estimation of the Differential Pathlength Factor for Human Skin Using Monte Carlo Simulations
Authors: Althobaiti Murad
Published: January 2023

Near-infrared technology is an emerging non-invasive technique utilized for various medical applications. Recently, there have been many attempts to utilize NIR technology for the continues monitoring of blood glucose levels through the skin. Different approaches and designs have been proposed for non-invasive blood glucose measurements. Light photons penetrating the skin can undergo multiple scattering events, and the actual optical pathlength becomes larger than the source-to-detector separation (optode spacing) in the reflection-mode configuration. Thus, the differential pathlength factor (DPF) must be incorporated into the modified Beer–Lambert law. The accurate estimation of the DPF values will lead to an accurate quantification of the physiological variations within the tissue. In this work, the aim was to systematically estimate the DPF for human skin for a range of source-to-detector separations and wavelengths. The Monte Carlo (MC) method was utilized to mimic the different layers of human skin with different optical properties and blood and water volume fractions. This work could help improve the accuracy of the near-infrared technique in the measurement of physiological variations within skin tissue.
Kinetics of the Cell Cycle Arrest Biomarkers (TIMP2 and IGFBP7) for the Diagnosis of Acute Kidney Injury in Critically Ill COVID-19 Patients
Authors: Greco Massimiliano , De Rosa Silvia , Boehm Fabian , Spano Sofia , Aceto Romina , Voza Antonio , Reggiani Francesco , Calatroni Marta , Castellani Gianluca , Costantini Elena , Villa Gianluca , Cecconi Maurizio
Published: January 2023

Background: Acute kidney injury (AKI) is highly prevalent in critical COVID-19 patients. The diagnosis and staging of AKI are based on serum creatinine (sCr) and urinary output criteria, with limitations in the functional markers. New cell-cycle arrest biomarkers [TIMP2]*[IGFBP7] have been proposed for early detection of AKI, but their role in critically ill COVID-19 patients is poorly understood. Methods: We conducted an observational study to assess the performance of [TIMP2]*[IGFBP7] for the detection of AKI in critical COVID-19 patients admitted to our intensive care unit (ICU). We sampled urinary [TIMP2]*[IGFBP7] levels at ICU admission, 12 h, 24 h, and 48 h, and compared the results to the development of AKI, as well as baseline and laboratory data. Results: Forty-one patients were enrolled. The median age was 66 years [57–72] and most were males (85%). Thirteen patients (31.7%) developed no/mild stage AKI, 19 patients (46.3%) moderate AKI, and nine patients (22.0%) severe AKI. The ICU mortality was 29.3%. sCr levels in the Emergency Department or at ICU admission were not significantly different according to AKI stage. [TIMP-2]*[IGFBP-7] urinary levels were elevated in severe AKI at 12 h after ICU admission, but not at ICU admission or 24 h or 48 h after ICU admission. Conclusion: Urinary biomarkers [TIMP-2]*[IGFBP-7] were generally increased in this population with a high prevalence of AKI, and were higher in patients with severe AKI measured at 12 h from ICU admission. Further studies are needed to evaluate the best timing of these biomarkers in this population.
Standardized Uptake Values on SPECT/CT: A Promising Alternative Tool for Treatment Evaluation and Prognosis of Metastatic Neuroendocrine Tumours
Authors: Gherghe Mirela , Lazar Alexandra Maria , Simion Laurentiu , Irimescu Ionela-Nicoleta , Sterea Maria-Carla , Mutuleanu Mario-Demian , Anghel Rodica Maricela
Published: January 2023

(1) Background: The aim of our study was to assess the feasibility of 99mTcEDDA/HYNIC-TOC SPECT/CT quantitative analysis in evaluating treatment response and disease progression in patients with NETs. (2) Methods: This prospective monocentric study evaluated 35 SPECT/CT examinations performed on 14 patients with neuroendocrine tumours who underwent a baseline and at least one follow-up 99mTcEDDA/HYNIC-TOC scan as part of their clinical management. The examination protocol included a whole-body scan acquired 2 h after the radiotracer’s administration, with the SPECT/CT performed 4 h post-injection. Images were analyzed by two experienced physicians and patients were classified into response categories based on their changes in SUV values. (3) Results: We evaluated 14 baseline studies and 21 follow-up scans, accounting for 123 lesions. A statistically positive correlation has been found between the SUVmax and SUVpeak values in tumoral lesions (p < 0.05). No correlation has been found between the SUV values and the ki67 proliferation index. Finally, 64.29% patients were classified as SD at the end of the study, with only 14.29% of patients exhibiting PD and 21.43% patients with PR. (4) Conclusions: The quantitative analysis of 99mTcEDDA/HYNIC-TOC SPECT/CT data in patients with neuroendocrine tumours could represent an alternative to 68Ga-DOTA-peptides PET/CT for the monitoring and prognosis of NETs.
Sex-Specific Cut-Offs of Single Point Insulin Sensitivity Estimator (SPISE) in Predicting Metabolic Syndrome in the Arab Adolescents
Authors: Wani Kaiser , Khattak Malak N. K. , Saadawy Gamal M. , Al-Attas Omar S. , Alokail Majed S. , Al-Daghri Nasser M.
Published: January 2023

The Single Point Insulin Sensitivity Estimator (SPISE) is a novel surrogate marker for insulin sensitivity and was found comparable to the gold standard clamp test as well as for predicting the Metabolic Syndrome (MetS) in several populations. The present study aimed to assess for the first time, the validity of SPISE in predicting MetS among Arab adolescents. In this cross-sectional study, 951 Saudi adolescents aged 10–17 years were randomly recruited from different schools across Riyadh, Saudi Arabia. Anthropometrics were measured and fasting blood samples were collected for the assessment of glucose, lipid profile, adipokines, C-reactive protein and 25 hydroxyvitamin (OH) D. MetS was defined using the National Cholesterol Education Program’s (NCEP) criteria with age-specific thresholds for adolescents. The SPISE as well as insulin resistance (HOMA-IR) indices were calculated. The over-all prevalence of MetS was 8.6% (82 out of 951). SPISE index was significantly lower in MetS than non-MetS participants in both sexes (5.5 ± 2.5 vs. 9.4 ± 3.2, p < 0.001 in boys and 4.4 ± 1.4 vs. 8.6 ± 3.2, p < 0.001 in girls). The SPISE index showed a significant inverse correlation with resistin, leptin, and C-reactive protein, and a significant positive correlation with adiponectin and 25(OH) D. Areas under the curve (AUC) revealed fair and good accuracy for predicting MetS 84.1% and 90.3% in boys and girls, respectively. The sex-specific cut-off proposed was SPISE index ≤6.1 (sensitivity 72.2% and specificity 83.9%) for boys and ≤6.46 (sensitivity 96.3% and specificity 73.4%), for girls. This study suggests that the SPISE index is a simple and promising diagnostic marker of insulin sensitivity and MetS in Arab adolescents.
Blockchain, Quo Vadis? Recent Changes in Perspectives on the Application of Technology in Agribusiness
Authors: Rocha Geneci da Silva Ribeiro , Mühl Diego Durante , Chingamba Hermenegildo Almeida , de Oliveira Letícia , Talamini Edson
Published: January 2023

Information technologies such as blockchain are developing fast, overcoming bottlenecks, and quickly taking advantage of their application. The present study analyzes recent changes concerning the benefits, disadvantages, challenges, and opportunities of blockchain applications in agribusiness. Interviews were conducted with and a questionnaire was applied to professionals working in the development and application of blockchain technology in agribusiness, to compare their perception of the recent advances. The results showed that the importance of blockchain technology to improve governance and information flow along supply chains has increased, and this is the main perceived benefit. The main disadvantages were removing intermediaries and the high cost of implementing the technology. The absence of a widely accepted platform in blockchain operations is the leading and growing challenge, while patterns for blockchain technology seem to be being overcome. The integration of blockchain with new technologies, and the competitiveness provided by the technology, are seen as the main and growing opportunities. Despite the study limitations, we conclude that the benefits and opportunities associated with blockchain application in agribusiness outweigh the challenges and disadvantages in number and importance, and are becoming more relevant.