bluuebunny/crossref_metadata_2025 huggingface dataset:
Multimodal Drumming Education Tool in Mixed Reality
Authors: Pinkl James , Villegas Julián , Cohen Michael
Published: August 2024

First-person VR- and MR-based Action Observation research has thus far yielded both positive and negative findings in studies observing such tools’ potential to teach motor skills. Teaching drumming, particularly polyrhythms, is a challenging motor skill to learn and has remained largely unexplored in the field of Action Observation. In this contribution, a multimodal tool designed to teach rudimental and polyrhythmic drumming was developed and tested in a 20-subject study. The tool presented subjects with a first-person MR perspective via a head-mounted display to provide users with visual exposure to both virtual content and their physical surroundings simultaneously. When compared against a control group practicing via video demonstrations, results showed increased rhythmic accuracy across four exercises. Specifically, a difference of 239 ms (z-ratio = 3.520, p < 0.001) was found between the timing errors of subjects who practiced with our multimodal mixed reality development compared to subjects who practiced with video, demonstrating the potential of such affordances. This research contributes to ongoing work in the fields of Action Observation and Mixed Reality, providing evidence that Action Observation techniques can be an effective practice method for drumming.
Tunable Ag Nanocavity Enhanced Green Electroluminescence from SiNx:O Light-Emitting Diode
Authors: Zuo Zongyan , Ma Zhongyuan , Chen Tong , Zhang Wenping , Li Wei , Xu Jun , Xu Ling , Chen Kunji
Published: August 2024

As the driving source, highly efficient silicon-based light emission is urgently needed for the realization of optoelectronic integrated chips. Here, we report that enhanced green electroluminescence (EL) can be obtained from oxygen-doped silicon nitride (SiNx:O) films based on an ordered and tunable Ag nanocavity array with a high density by nanosphere lithography and laser irradiation. Compared with that of a pure SiNxO device, the green electroluminescence (EL) from the SiNx:O/Ag nanocavity array device can be increased by 7.1-fold. Moreover, the external quantum efficiency of the green electroluminescence (EL) is enhanced 3-fold for SiNx:O/Ag nanocavity arrays with diameters of 300 nm. The analysis of absorption spectra and the FDTD calculation reveal that the localized surface plasmon (LSP) resonance of size-controllable Ag nanocavity arrays and SiNx:O films play a key role in the strong green EL. Our discovery demonstrates that SiNx:O films coupled with tunable Ag nanocavity arrays are promising for silicon-based light-emitting diode devices of the AI period in the future.
A Mid-Infrared Perfect Metasurface Absorber with Tri-Band Broadband Scalability
Authors: Zou Yongtu , Zhou Shaolin , Li Jingxi , Chen Shanri , Chen Zhijian
Published: August 2024

Metasurfaces have emerged as a unique group of two-dimensional ultra-compact subwavelength devices for perfect wave absorption due to their exceptional capabilities of light modulation. Nonetheless, achieving high absorption, particularly with multi-band broadband scalability for specialized scenarios, remains a challenge. As an example, the presence of atmospheric windows, as dictated by special gas molecules in different infrared regions, highly demands such scalable modulation abilities for multi-band absorption and filtration. Herein, by leveraging the hybrid effect of Fabry–Perot resonance, magnetic dipole resonance and electric dipole resonance, we achieved multi-broadband absorptivity in three prominent infrared atmospheric windows concurrently, with an average absorptivity of 87.6% in the short-wave infrared region (1.4–1.7 μm), 92.7% in the mid-wave infrared region (3.2–5 μm) and 92.4% in the long-wave infrared region (8–13 μm), respectively. The well-confirmed absorption spectra along with its adaptation to varied incident angles and polarization angles of radiations reveal great potential for fields like infrared imaging, photodetection and communication.
New Nitrogen Use Efficiency Indices for Biomass Formation and Productivity in Green Beans Under Foliar Fertilization with Molybdenum Nanofertilizer
Authors: Muñoz-Márquez Ezequiel , Soto-Parra Juan Manuel , Pérez-Leal Ramona , Sanchez Esteban
Published: August 2024

Most crops are fertilized with high amounts of nitrogen, and have an alarmingly low utilization efficiency. For this reason, the coordination between the fertilizer contribution and the nitrogen requirements of the crop is very important. Therefore, the objective of the present study was to establish new indices to determine nitrogen use efficiency (NUE), and to define the amount of assimilated nitrogen, which is used for the formation of green bean plant organs, fertilized with molybdenum nanofertilizer applied foliarly, and combined with edaphic fertilization of ammonium nitrate. The plants were grown in a greenhouse covered with anti-aphid mesh and irrigated with nutrient solution. Three sources of foliar molybdenum (Nanofertilizer, molybdenum Chelate and Sodium Molybdate) were applied in four doses of 0, 5, 10 and 20 ppm Mo, complemented with edaphic fertilization of NH4NO3 (0, 3, 6 and 12 mM of N). As results, the NUE indices showed that with the application of the nanofertilizer, the total biomass production increased 41.65% more than with the application of the chelate, and 36.84% more than with the application of molybdate. In summary, the dose that presented the highest efficiency was 6 mM-N with 10 ppm-Mo. Finally, it is concluded that the use of NUE indices is an important approach that evaluates the fate of nitrogen and accurately estimates plant yield.
Healthy Dietary Patterns with and without Meat Improved Cardiometabolic Disease Risk Factors in Adults: A Randomized Crossover Controlled Feeding Trial
Authors: Hill Erica R , Wang Yu , Davis Eric M , Campbell Wayne W
Published: August 2024

We assessed the effects of consuming a U.S.-style healthy dietary pattern (HDP) with lean, unprocessed beef (BEEF) compared to a U.S.-style HDP without meat (vegetarian, VEG) on short-term changes in cardiometabolic disease (CMD) risk factors in adults classified as overweight or obese. Forty-one adults (22 females, 19 males; age 39.9 ± 8.0 y; BMI 29.6 ± 3.3 kg/m2; mean ± SD) completed two 5-week controlled feeding periods (randomized, crossover, controlled trial). For the BEEF HDP, two 3-oz (168-g) servings/d of lean, unprocessed beef were predominately substituted for some starchy vegetables and refined grains in the VEG HDP. Baseline and post-intervention measurements were fasting CMD risk factors, with serum low-density lipoprotein (LDL), total cholesterol (TC), and total apolipoprotein B as primary outcomes. VEG reduced LDL, insulin, and glucose compared to BEEF. Reductions did not differ between VEG vs. BEEF for TC, high-density lipoprotein (HDL), apolipoprotein A1, small, dense LDL IV, buoyant HDL2b, TC-to-HDL ratio, and systolic blood pressure. Total apolipoprotein B and all other CMD risk factors measured were not influenced by HDP type nor changed over time. Adopting a U.S.-style HDP that is either vegetarian or omnivorous with beef improved multiple cardiometabolic disease risk factors among adults classified as overweight or obese.
Longitudinal Changes in Dietary Supplement Use among United States Military Personnel: The US Military Dietary Supplement Use Study
Authors: Knapik Joseph , Trone Daniel , Steelman Ryan , Farina Emily , Lieberman Harris
Published: August 2024

Previous studies investigating temporal changes in dietary supplement (DS) use have used representative samples but have not followed the same cohort over time. This study investigated longitudinal patterns of changes in DS use and factors associated with discontinuing DS use in a single group of active-duty United States military service members (SMs). SMs (n = 5778) completed two identical questionnaires on their DS use and demographic/lifestyle characteristics an average ± standard deviation 1.3 ± 0.2 years apart. Prevalences of reported DS use ≥1 times/week in the baseline (BL) and follow-up (FU) phases were: any DS, BL = 77%, FU = 78%; multivitamins/multiminerals (MVM), BL = 50%, FU = 48%; individual vitamins/minerals, BL = 33%, FU 35%; proteins/amino acids, BL = 43%, FU = 39%; combination products, BL = 44%, FU = 37%; prohormones, BL = 5%, FU = 4%; herbal products, BL = 23%, FU = 21%; joint health products, BL = 12%, FU = 12%; fish oils, BL = 25%, FU = 23%; other DSs, BL = 17%, FU = 17%. Among BL users, the proportions reporting use in the FU phase were: any DS 88%, MVM 74%, protein/amino acids 70%, individual vitamin/minerals 62%, combination products 62%, fish oils 61%, joint health products 57%, herbal products 50%, other DSs 50%, and prohormones 37%. Higher odds of discontinuing any DS use in the follow-up were associated with female gender, younger age, higher BMI, and less weekly resistance training. Overall, prevalence of DS use was relatively consistent in the two phases; however, the cohort changed their use patterns in the follow-up with some discontinuing use and others initiating use, thus maintaining use prevalence over the period. These findings have implications for repeated cross-sectional DS studies where different samples are followed over time.
The Gender Gap in the Relationship between Metabolic Syndrome and Restrictive Ventilatory Defects
Authors: Chu Ya-Chun , Yang Chi-Chiang , Chen Shaw-Ji , Cheng Pei-Ling , Wu Mei-Chuan , Wu Hsin-Hung , Lai Cheng-Yen
Published: August 2024

Background: Given the fundamental physiological differences between the sexes, this study aimed to investigate the effect of metabolic syndrome on ventilatory defects stratified by sex. Methods: We conducted a nationwide, pooled, cross-sectional study. Data from 45,788 participants (men, n = 15,859; women, n = 29,929) aged 30 years or more were obtained from the Taiwan Biobank. Age–sex-adjusted and multivariate logistic regression models were used to estimate the risk of developing impaired pulmonary function (restrictive or obstructive ventilatory defects) in individuals with or without metabolic syndromes. Separate models were also used to estimate the effect of metabolic syndrome scores and the effect of individual metabolic abnormalities on the risk of restrictive ventilatory defects. Results: The overall prevalence of metabolic syndrome was estimated to be 15.9% in Taiwan, much higher in men than in women (18.6% versus 14.4%). A significant association was observed between metabolic syndromes and the risk of restrictive ventilatory defects. The risk of developing a restrictive ventilator defect was 35% higher in participants with metabolic syndromes (odds ratio, 1.35; 95% confidence interval, 1.26–1.45) than in those without metabolic syndromes. Elevated blood pressure and a triglycerides abnormality were important predictors of restrictive ventilator defects. Sex-stratified subgroup analyses of the individual metabolic abnormalities indicated that men with abdominal obesity and women with dysglycemia were more likely to develop restrictive ventilatory defects. Conclusions: Our study’s evidence suggested that metabolic syndromes were important predictors of impaired pulmonary function and an increased risk of developing restrictive ventilatory defects, and its risk increased with increasing numbers of metabolic abnormalities.
A Mixture of Lactobacillus HY7601 and KY1032 Regulates Energy Metabolism in Adipose Tissue and Improves Cholesterol Disposal in High-Fat-Diet-Fed Mice
Authors: Lee Kippeum , Kim Hyeon-Ji , Kim Joo-Yun , Shim Jae-Jung , Lee Jae-Hwan
Published: August 2024

We aimed to characterize the anti-obesity and anti-atherosclerosis effects of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 using high-fat diet (HFD)-fed obese C57BL/6 mice. We divided the mice into control (CON), HFD, HFD with 108 CFU/kg/day probiotics (HFD + KL, HY7301:KY1032 = 1:1), and HFD with 109 CFU/kg/day probiotics (HFD + KH, HY7301:KY1032 = 1:1) groups and fed/treated them during 7 weeks. The body mass, brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal white adipose tissue (eWAT) masses and the total cholesterol and triglyceride concentrations were remarkably lower in probiotic-treated groups than in the HFD group in a dose-dependent manner. In addition, the expression of uncoupling protein 1 in the BAT, iWAT, and eWAT was significantly higher in probiotic-treated HFD mice than in the HFD mice, as demonstrated by immunofluorescence staining and Western blotting. We also measured the expression of cholesterol transport genes in the liver and jejunum and found that the expression of those encoding liver-X-receptor α, ATP-binding cassette transporters G5 and G8, and cholesterol 7α-hydroxylase were significantly higher in the HFD + KH mice than in the HFD mice. Thus, a Lactobacillus HY7601 and KY1032 mixture with 109 CFU/kg/day concentration can assist with body weight regulation through the management of lipid metabolism and thermogenesis.
The Effect of 4-Month Treatment with Glycocalyx Dietary Supplement on Endothelial Glycocalyx Integrity and Vascular Function in Patients with Psoriasis
Authors: Ikonomidis Ignatios , Katsanaki Eleni , Thymis John , Pavlidis George , Lampadaki Kyriaki , Katogiannis Konstantinos , Vaiopoulos Aristeidis , Lazarou Vicky , Kostelli Gavriella , Michalopoulou Eleni , Pililis Sotirios , Vlachomitros Dimitrios , Theodoropoulos Konstantinos , Vink Hans , Long Robert , Papadavid Evangelia , Lambadiari Vaia
Published: August 2024

Psoriasis predisposes to cardiovascular dysfunction. We investigated whether glycocalyx dietary supplement (GDS), which contains glycosaminoglycans and fucoidan, improves endothelial glycocalyx and arterial stiffness in psoriatic patients. Fifty participants with psoriasis under biological agents were randomly assigned to GDS (n = 25) or placebo (n = 25) for 4 months. We measured at baseline and at follow-up: (a) perfused boundary region (PBR) of the sublingual microvessels (range 4 to 25 μm), a marker of endothelium glycocalyx integrity; (b) carotid–femoral pulse wave velocity (PWV-Complior SP-ALAM) and augmentation index (AIx), markers of arterial stiffness and (c) psoriasis area and severity index (PASI) score. Both groups displayed a similar decrease in PASI at four months (p < 0.05), and no significant differences were found between groups (p > 0.05). Compared to the placebo, participants in the GDS showed a greater percentage reduction in PBR4–25 μm (−9.95% vs. −0.87%), PBR 4–9 μm (−6.50% vs. −0.82%), PBR10–19 μm (−5.12% vs. −1.60%), PBR 20–25 μm (−14.9% vs. −0.31%), PWV (−15.27% vs. −4.04%) and AIx (−35.57% vs. −21.85%) (p < 0.05). In the GDS group, the percentage reduction in PBR 4–25 μm was associated with the corresponding decrease in PWV (r = 0.411, p = 0.015) and AΙx (r = 0.481, p = 0.010) at follow-up. Four-month treatment with GDS improves glycocalyx integrity and arterial stiffness in patients with psoriasis. Clinical trial Identifier: NCT05184699.
Host-Pathogen Interaction and Resistance Mechanisms in Dermatophytes
Authors: Dubljanin Eleonora , Zunic Jelena , Vujcic Isidora , Colovic Calovski Ivana , Sipetic Grujicic Sandra , Mijatovic Stefan , Dzamic Aleksandar
Published: August 2024

Dermatophytes are widely distributed in the environment, with an estimated prevalence of 20–25% of the the global population yearly. These fungi are keratinophilic and keratinolytic and cause the infection of keratin-rich structures such as skin, hair, and nails. The pattern of this infectious disease covers a wide spectrum from exposed individuals without symptoms to those with acutely inflammatory or non-inflammatory, chronic to invasive, and even life-threatening symptoms. This review summarizes current information on the pathogenicity, virulence factors, and drug resistance mechanisms associated with dermatophytes. A greater number of virulence factors of these fungi are important for the occurrence of infection and the changes that occur, including those regarding adhesins, the sulfite efflux pump, and proteolytic enzymes. Other virulence factors include mechanisms of evading the host defense, while the development of resistance to antifungal drugs is increasing, resulting in treatment failure. The investigation of host-pathogen interactions is essential for developing a more complete understanding of the mechanisms underlying dermatophyte pathogenesis and host response to inform the use of diagnostics methods and antifungal therapeutics to minimize the high fungal burden caused by dermatophytes and to control the spread of resistance.
Is There an Interplay between Environmental Factors, Microbiota Imbalance, and Cancer Chemotherapy-Associated Intestinal Mucositis?
Authors: Fernandes Camila , Miranda Mahara Coelho Crisostomo , Roque Cássia Rodrigues , Paguada Ana Lizeth Padilla , Mota Carlos Adrian Rodrigues , Florêncio Katharine Gurgel Dias , Pereira Anamaria Falcão , Wong Deysi Viviana Tenazoa , Oriá Reinaldo Barreto , Lima-Júnior Roberto César Pereira
Published: August 2024

Interindividual variation in drug efficacy and toxicity is a significant problem, potentially leading to adverse clinical and economic public health outcomes. While pharmacogenetics and pharmacogenomics have long been considered the primary causes of such heterogeneous responses, pharmacomicrobiomics has recently gained attention. The microbiome, a community of microorganisms living in or on the human body, is a critical determinant of drug response and toxicity. Factors such as diet, lifestyle, exposure to xenobiotics, antibiotics use, illness, and genetics can influence the composition of the microbiota. Changes in the intestinal microbiota are particularly influential in drug responsiveness, especially in cancer chemotherapy. The microbiota can modulate an individual’s response to a drug, affecting its bioavailability, clinical effect, and toxicity, affecting treatment outcomes and patient quality of life. For instance, the microbiota can convert drugs into active or toxic metabolites, influencing their efficacy and side effects. Alternatively, chemotherapy can also alter the microbiota, creating a bidirectional interplay. Probiotics have shown promise in modulating the microbiome and ameliorating chemotherapy side effects, highlighting the potential for microbiota-targeted interventions in improving cancer treatment outcomes. This opinion paper addresses how environmental factors and chemotherapy-induced dysbiosis impact cancer chemotherapy gastrointestinal toxicity.
Bioequivalence Analysis of Terazosin Hydrochloride Tablets Based on Parallel Artificial Membrane Permeability Analysis
Authors: Niu Jianzhao , Huang Hanhan , Ji Ming , Zhang Wenjing , Huang Yin , Ma Lingyun , Wang Baolian , Liu Qian
Published: August 2024

Parallel artificial membrane permeability analysis (PAMPA) is used to determine the permeability of compounds through concentrated negatively charged phospholipid bilayer barriers. We employed MacroFlux (a scaled-up version of PAMPA) to test the permeation rate of terazosin hydrochloride (TH) tablets and predict in vivo bioequivalence. The dissolution profiles and permeability of one reference formulation, and seven generic TH tablets, were compared. The dissolution profiles of these generic tablets were equivalent to that of the reference drug in four different media. However, the flux and the total permeated amount of some generic TH tablets were below the lower limit of the confidence interval of the original acceptance range in MacroFlux, which implied risk in the bioequivalence test in vivo. We further evaluated potential factors responsible for this discrepancy by µFlux, including active pharmaceutical ingredient (API) permeability and excipient prescriptions. The analysis showed that different properties of API were a main factor leading to biological inequivalence in the MacroFlux assay, while excipient prescriptions did not have an impact on bioequivalence risk. These data indicated that the flux assay may be a helpful as an auxiliary method for predicting bioequivalence of generic drugs and analyze the factors responsible for bioequivalence risk.
Potentially Inappropriate Medications Involved in Drug–Drug Interactions in a Polish Population over 80 Years Old: An Observational, Cross-Sectional Study
Authors: Błeszyńska-Marunowska Emilia , Jagiełło Kacper , Wierucki Łukasz , Renke Marcin , Grodzicki Tomasz , Kalarus Zbigniew , Zdrojewski Tomasz
Published: August 2024

The clinical context of drug interactions detected by automated analysis systems is particularly important in older patients with multimorbidities. We aimed to provide unique, up-to-date data on the prevalence of potentially inappropriate medications (PIMs) and drug–drug interactions (DDIs) in the Polish geriatric population over 80 years old and determine the frequency and the most common PIMs involved in DDIs. We analyzed all non-prescription and prescription drugs in a representative national group of 178 home-dwelling adults over 80 years old with excessive polypharmacy (≥10 drugs). The FORTA List was used to assess PIMs, and the Lexicomp® Drug Interactions database was used for DDIs. DDIs were detected in 66.9% of the study group, whereas PIMs were detected in 94.4%. Verification of clinical indications for the use of substances involved in DDIs resulted in a reduction in the total number of DDIs by more than 1.5 times, as well as in a nearly 3-fold decrease in the number of interactions requiring therapy modification and drug combinations that should be strictly avoided. The most common PIMs involved in DDIs were painkillers, and drugs used in psychiatry and neurology. Special attention should be paid to DDIs with PIMs since they could increase their inappropriate character. The use of automated interaction analysis systems, while maintaining appropriate clinical criticism, can increase both chances for a good therapeutic effect and the safety of the elderly during treatment processes.
Adverse Event Profiles of Adalimumab in Children: A Disproportionality Analysis
Authors: Zhang Wenting , Xu Ziqi , Shu Yamin , Shu Sainan , Zhang Qilin
Published: August 2024

Background: Adalimumab has been approved by the U.S. Food and Drug Administration (FDA) for the treatment of adult rheumatoid arthritis (RA), and subsequently approved for pediatric treatment of various autoimmune diseases in children of different ages. Due to genetic differences between children and adults in terms of physiology and immunity, there is a need to explore the safety of adalimumab in children in the real world. The aim of this study is to identify potential adverse event (AE) signals associated with the use of adalimumab in pediatric patients (<18 years old) using data from the FDA Adverse Event Reporting System (FAERS). Methods: AEs associated with adalimumab in pediatric patients reported in the FAERS database from the first quarter (Q1) of 2017 to the third quarter (Q3) of 2022 were systematically gathered. Reporting odds ratio (ROR), the proportional reporting ratio (PRR), the information component (IC), and the empirical Bayes geometric mean (EBGM) were used to assess the relationship between adalimumab and AEs in children. Results: Out of 8,363,304 reports collected from the FAERS database during the study period, 3819 reports on children on adalimumab were identified. Adalimumab-related AEs reports were concentrated on 10 toxicity areas and a total of 202 positive signals were detected, of which injection site papule (ROR = 261.97) and intestinal fistula (ROR = 122.09) had the strongest signals. Unexpected significant AEs, including intestinal obstruction, immunodeficiency, abdominal abscess, and Takayasu’s arteritis might also occur. In comparison with patients of all ages in the same time window, the median onset time of children was shorter (99 vs. 149 days). Most of the AE cases occurred in children within the first 1 (1.71%), 2 (8.12%), and 3 months (8.39%) and had early failure types after adalimumab initiation. Methotrexate, folic acid, prednisone, azathioprine, and mesalamine were the top five drugs used concomitantly for adalimumab-associated AEs. Conclusions: When adalimumab is used in children, especially in the first 3 months of treatment, in addition to the AEs recorded in the drug package insert, close attention should be paid to the new potential AEs off-label to ensure the safety of adalimumab in children.
The Pathogenic Mechanisms of and Novel Therapies for Lamin A/C-Related Dilated Cardiomyopathy Based on Patient-Specific Pluripotent Stem Cell Platforms and Animal Models
Authors: Wu Xin-Yi , Lee Yee-Ki , Lau Yee-Man , Au Ka-Wing , Tse Yiu-Lam , Ng Kwong-Man , Wong Chun-Ka , Tse Hung-Fat
Published: August 2024

Variants (pathogenic) of the LMNA gene are a common cause of familial dilated cardiomyopathy (DCM), which is characterised by early-onset atrioventricular (AV) block, atrial fibrillation and ventricular tachyarrhythmias (VTs), and progressive heart failure. The unstable internal nuclear lamina observed in LMNA-related DCM is a consequence of the disassembly of lamins A and C. This suggests that LMNA variants produce truncated or alternative forms of protein that alter the nuclear structure and the signalling pathway related to cardiac muscle diseases. To date, the pathogenic mechanisms and phenotypes of LMNA-related DCM have been studied using different platforms, such as patient-specific induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs) and transgenic mice. In this review, point variants in the LMNA gene that cause autosomal dominantly inherited forms of LMNA-related DCM are summarised. In addition, potential therapeutic targets based on preclinical studies of LMNA variants using transgenic mice and human iPSC-CMs are discussed. They include mitochondria deficiency, variants in nuclear deformation, chromatin remodelling, altered platelet-derived growth factor and ERK1/2-related pathways, and abnormal calcium handling.
Synthesis and Biochemical Evaluation of Ethanoanthracenes and Related Compounds: Antiproliferative and Pro-Apoptotic Effects in Chronic Lymphocytic Leukemia (CLL)
Authors: McKeown James , Byrne Andrew , Bright Sandra , Charleton Clara , Kandwal Shubhangi , Čmelo Ivan , Twamley Brendan , McElligott Anthony , Fayne Darren , O’Boyle Niamh , Williams D. , Meegan Mary
Published: August 2024

Chronic lymphocytic leukemia (CLL) is a malignancy of mature B cells, and it is the most frequent form of leukemia diagnosed in Western countries. It is characterized by the proliferation and accumulation of neoplastic B lymphocytes in the blood, lymph nodes, bone marrow and spleen. We report the synthesis and antiproliferative effects of a series of novel ethanoanthracene compounds in CLL cell lines. Structural modifications were achieved via the Diels–Alder reaction of 9-(2-nitrovinyl)anthracene and 3-(anthracen-9-yl)-1-arylprop-2-en-1-ones (anthracene chalcones) with dienophiles, including maleic anhydride and N-substituted maleimides, to afford a series of 9-(E)-(2-nitrovinyl)-9,10-dihydro-9,10-[3,4]epipyrroloanthracene-12,14-diones, 9-(E)-3-oxo-3-phenylprop-1-en-1-yl)-9,10-dihydro-9,10-[3,4]epipyrroloanthracene-12,14-diones and related compounds. Single-crystal X-ray analysis confirmed the structures of the novel ethanoanthracenes 23f, 23h, 24a, 24g, 25f and 27. The products were evaluated in HG-3 and PGA-1 CLL cell lines (representative of poor and good patient prognosis, respectively). The most potent compounds were identified as 20a, 20f, 23a and 25n with IC50 values in the ranges of 0.17–2.69 µM (HG-3) and 0.35–1.97 µM (PGA-1). The pro-apoptotic effects of the potent compounds 20a, 20f, 23a and 25n were demonstrated in CLL cell lines HG-3 (82–95%) and PGA-1 (87–97%) at 10 µM, with low toxicity (12–16%) observed in healthy-donor peripheral blood mononuclear cells (PBMCs) at concentrations representative of the compounds IC50 values for both the HG-3 and PGA-1 CLL cell lines. The antiproliferative effect of the selected compounds, 20a, 20f, 23a and 25n, was mediated through ROS flux with a marked increase in cell viability upon pretreatment with the antioxidant NAC. 25n also demonstrated sub-micromolar activity in the NCI 60 cancer cell line panel, with a mean GI50 value of 0.245 µM. This ethanoanthracene series of compounds offers potential for the further development of lead structures as novel chemotherapeutics to target CLL.
New Approaches for Basophil Activation Tests Employing Dendrimeric Antigen–Silica Nanoparticle Composites
Authors: Calvo-Serrano Silvia , Matamoros Esther , Céspedes Jose Antonio , Fernández-Santamaría Rubén , Gil-Ocaña Violeta , Perez-Inestrosa Ezequiel , Frecha Cecilia , Montañez Maria I. , Vida Yolanda , Mayorga Cristobalina , Torres Maria J.
Published: August 2024

In vitro cell activation through specific IgE bound to high-affinity receptors on the basophil surface is a widely used strategy for the evaluation of IgE-mediated immediate hypersensitivity reactions to betalactams. Cellular activation requires drug conjugation to a protein to form a large enough structure displaying a certain distance between haptens to allow the cross-linking of two IgE antibodies bound to the basophil’s surface, triggering their degranulation. However, no information about the size and composition of these conjugates is available. Routine in vitro diagnosis using the basophil activation test uses free amoxicillin, which is assumed to conjugate to a carrier present in blood. To standardize the methodology, we propose the use of well-controlled and defined nanomaterials functionalized with amoxicilloyl. Silica nanoparticles decorated with PAMAM–dendrimer–amoxicilloyl conjugates (NpDeAXO) of different sizes and amoxicilloyl densities (50–300 µmol amoxicilloyl/gram nanoparticle) have been prepared and chemically characterized. Two methods of synthesis were performed to ensure reproducibility and stability. Their functional effect on basophils was measured using an in-house basophil activation test (BAT) that determines CD63+ or CD203chigh activation markers. It was observed that NpDeAXO nanocomposites are not only able to specifically activate basophils but also do so in a more effective way than free amoxicillin, pointing to a translational potential diagnosis.
Melanoma Brain Metastases Patient-Derived Organoids: An In Vitro Platform for Drug Screening
Authors: Abedellatif Saif-Eldin , Hosni Racha , Waha Andreas , Gielen Gerrit H. , Banat Mohammed , Hamed Motaz , Güresir Erdem , Fröhlich Anne , Sirokay Judith , Wulf Anna-Lena , Kristiansen Glen , Pietsch Torsten , Vatter Hartmut , Hölzel Michael , Schneider Matthias , Toma Marieta Ioana
Published: August 2024

Background and aims: Brain metastases are prevalent in the late stages of malignant melanoma. Multimodal therapy remains challenging. Patient-derived organoids (PDOs) represent a valuable pre-clinical model, faithfully recapitulating key aspects of the original tumor, including the heterogeneity and the mutational status. This study aimed to establish PDOs from melanoma brain metastases (MBM-PDOs) and to test the feasibility of using them as a model for in vitro targeted-therapy drug testing. Methods: Surgical resection samples from eight patients with melanoma brain metastases were used to establish MBM-PDOs. The samples were enzymatically dissociated followed by seeding into low-attachment plates to generate floating organoids. The MBM-PDOs were characterized genetically, histologically, and immunohistologically and compared with the parental tissue. The MBM-PDO cultures were exposed to dabrafenib (BRAF inhibitor) and trametinib (MEK inhibitor) followed by a cell viability assessment. Results: Seven out of eight cases were successfully cultivated, maintaining the histological, immunohistological phenotype, and the mutational status of the parental tumors. Five out of seven cases harbored BRAF V600E mutations and were responsive to BRAF and MEK inhibitors in vitro. Two out of seven cases were BRAF wild type: one case harboring an NRAS mutation and the other harboring a KIT mutation, and both were resistant to BRAF and MEK inhibitor therapy. Conclusions: We successfully established PDOs from melanoma brain metastases surgical specimens, which exhibited a consistent histological and mutational profile with the parental tissue. Using FDA-approved BRAF and MEK inhibitors, our data demonstrate the feasibility of employing MBM-PDOs for targeted-therapy in vitro testing.
The Communicology of a Blank Paper, a Void That Expresses
Authors: Wang Hong
Published: August 2024

In this paper I attempt to trace the semiotic path of meaning experience from “nothing” into “something”. Traditional communication studies are problematic in 1. focusing on the message to the effect of ignoring the communicators; 2. choosing to overlook how yet-to-be signs acquire meanings in the communicative moments; and 3. tending to assume a “natural science” attitude toward the studied phenomenon so that embodied consciousness is either sidetracked or psychologized. Taking communicology as both the theory and methodology, I first describe the semiotic network in which blank paper, a nonconventional sign, acquires its signness in a specified communicative event. Then, I look inward to the relation of consciousness and embodiment. Finally, I argue that communication is such a life-world moment wherein non-expression is collectively constituted as a form of expression.
Laser-Tracing Multi-Station Measurements in a Non-Uniform-Temperature Field
Authors: Chen Hongfang , Zhang Ao , Sun Mengyang , Li Changcheng , Wu Huan , Liang Ziqi , Shi Zhaoyao
Published: August 2024

Due to the increasing requirements for the improvement of the accuracy of large coordinate-measuring machines (CMMs), the laser-tracing multi-station measurement technology, as one of the advanced precision measurement technologies, is worth studying in depth in terms of its practical application for the compensation of errors in large CMMs. Since it is difficult to maintain a constant temperature of about 20 °C in the actual workshop under the influence of solar radiation and convective heat transfer, there is a gradient in the spatial temperature distribution, and the overall temperature changes with the influence of external factors with synchronous hysteresis, it is difficult for the actual calibration environment to meet the standard environmental requirements. Therefore, the influence of temperature and other environmental factors on the accuracy of laser ranging and large-scale CMM calibration should not be ignored. In this paper, on the basis of analyzing the temperature distribution and change rule of large CMM measurement space under different working conditions, the radial basis function (RBF) neural network algorithm was used to build a non-uniform-temperature field model, and based on this model and the measurement principle of the laser-tracking instrument, the method of laser tracking and interferometric ranging accuracy enhancement was put forward under a non-uniform-temperature field. Finally, based on the multi-station technique of laser tracing, an accurate solution for the volumetric error of large CMMs under the condition of non −20 °C ambient temperature was realized. Simulation results proved that compared with the traditional temperature-compensation method, the proposed method improved the measurement accuracy of the volumetric error of a large-scale CMM using laser-tracing multi-station technology in a non-uniform-temperature field by 33.5%. This study provides a new approach for improving the accuracy of laser-tracer multi-station measurement systems.
Reducing Multiphoton Noise in Multiplexed Single-Photon Sources
Authors: Adam Peter , Mechler Matyas
Published: August 2024

Multiplexed single-photon sources can produce indistinguishable single photons with high probability in near-perfect spatial modes. Such systems, realized with optical elements having losses, can be optimized—that is, both the optimal number of multiplexed units in the sources and the optimal mean number of photon pairs generated in a multiplexed unit, for which the output single-photon probability is maximal, can be determined. The accompanying multiphoton noise of the sources, arising from the probabilistic nature of the underlying physical processes in these systems, can be detrimental in certain applications. Inspired by this fact, we develop a procedure aimed at decreasing the multiphoton noise of multiplexed single-photon sources. The procedure is based on the reoptimization of the system for the chosen value of the normalized second-order autocorrelation function characterizing the multiphoton noise. The results of this reoptimization are shown for two types of spatially multiplexed single-photon sources. We find that by applying the proposed procedure, the multiphoton noise can be considerably decreased along with a relatively low decrease in the single-photon probability. Although the method presented here is for two spatially multiplexed single-photon sources, it can be applied straightforwardly for any type of multiplexed single-photon source.
Vibration-Induced Sweeping Operation in Fiber Lasers
Authors: Kashirina Ekaterina K. , Lobach Ivan A. , Kablukov Sergey I.
Published: August 2024

A new vibration-based mechanism of optical frequency/wavelength sweeping in fiber lasers induced by optical path length modulation of a laser cavity section is proposed. The mechanism is implemented for an erbium-doped fiber ring laser with a saturable absorber. Without the vibrations, the laser generates a single longitudinal mode (SLM) radiation. We show experimentally for the first time that mechanical vibrations of the laser cavity section can lead to mode dynamics in both frequency and time domains. The possibility of obtaining various mode dynamics, such as vibration-induced sweeping in a wavelength range of up to 2.2 nm or SLM generation with periodic mode hopping between two fixed longitudinal modes depending on the pump wavelength, is experimentally shown. In this vibration-based approach, the interval between changes in the laser cavity modes has good stability, because it directly relates to the vibration period.
The Influence of Logging-Related Soil Disturbance on Pioneer Tree Regeneration in Mixed Temperate Forests
Authors: Tavankar Farzam , Venanzi Rachele , Nikooy Mehrdad , Lo Monaco Angela , Picchio Rodolfo , Naghdi Ramin
Published: August 2024

The recovery of soil properties and the proper growth of natural tree regeneration are key elements for maintaining forest productivity after selective logging operations. This study was conducted on the soil properties and natural growth of two pioneer seedling species of alder and maple which were on skid trails in the mixed beech forests of northern Iran. To examine the long-term effects, we randomly selected six skid trails, with two replicates established for each of three time periods since last use (10, 20, and 30 years ago). Random plots 4 m × 10 m in size, three plots on each skid trail and six plots on areas without soil compaction (control), were selected. Measurements included the physical and chemical properties of the soil and the growth, and the architectural and qualitative characteristics of the seedlings. The results showed that all the soil properties of the 10- and 20-year-old skid trails were significantly different from the control area (except for the soil moisture in the 20-year-old skid trail). The 30-year-old skid trail showed values of other soil properties which were not significantly different from the control area, except for the amounts of organic matter and soil nitrogen, which was less than the control. The skid trails had a negative effect on all of the growth, qualitative, and architectural indices of seedlings. The characteristics of seedlings were related to soil characteristics and had the highest correlation with the soil penetration resistance (R-value from −0.41 to −0.63 for stem growth, p < 0.05; −0.57 to −0.90 for root growth, p < 0.01; and −0.76 to −0.86 for biomass, p < 0.01). The correlation coefficient between soil penetration resistance and the Dickson quality index of alder and maple seedlings was, respectively, −0.74 and −0.72, p < 0.01. The negative effect of soil compaction on root growth (−27.69% for alder seedlings and −28.08% for maple seedlings) was greater than on stem growth (−24.11% for alder seedlings and −16.27% for maple seedlings). The amount of growth, qualitative, and architectural indices of alder seedlings were higher than that of maple seedlings. Although alder is a better choice as compared to maple seedling in the initial year, the results of our study show that it is recommended to plant both alder and maple on skid trails after logging operations.
Assessing the Fates of Water and Nitrogen on an Open-Field Intensive Vegetable System under an Expert-N System with EU-Rotate_N Model in North China Plain
Authors: Sun Yuan , Chen Shaoqing , Feng Puyu , Chen Qing , Hu Kelin
Published: August 2024

Nitrate leaching, greenhouse gas emissions, and water loss are caused by conventional water and fertilizer management in vegetable fields. The Expert-N system is a useful tool for recommending the optimal nitrogen (N) fertilizer for vegetable cultivation. To clarify the fates of water and N in vegetable fields, an open-field vegetable cultivation experiment was conducted in Dongbeiwang, Beijing. This experiment tested two irrigation treatments (W1: conventional and W2: optimal) and three fertilizer treatments (N1: conventional, N2: optimal N rate by Expert-N system, and N3: 80% optimal N rate) on cauliflower (Brassica oleracea L.), amaranth (Amaranthus tricolor L.), and spinach (Spinacia oleracea L.). The EU-Rotate_N model was used to simulate the fates of water and N in the soil. The results indicated that the yields of amaranth and spinach showed no significant differences among all the treatments in 2000 and 2001. However, cauliflower yield under the W1N2 and W1N3 treatments obviously reduced in 2001. Compared with the W1 treatment, W2 reduced irrigation amount by 27.9–29.8%, water drainage by over 76%, increased water use efficiency by 5–17%, and irrigation water use efficiency by 29–45%. Nitrate leaching was one of the main pathways in this study, accounting for 8.4% of the total N input; compared to N1, the input of fertilizer N under the N2 and N3 treatments decreased by over 66.5%, consequently reducing gaseous N by 48–72% and increasing nitrogen use efficiency (NUE) by 17–37%. Additionally, compared with the W1 treatments, gaseous N loss under the W2 treatments was reduced by 18–26% and annual average NUEs increased by 22–29%. The highest annual average NUEs were under W2N3 (169.6 kg kg−1) in 2000 and W2N2 (188.0 kg kg−1) in 2001, respectively. We found that optimizing fertilizer management allowed subsequent crops to utilize residual N in the soil. Therefore, we suggest that the W2N3 management should be recommended to farmers to reduce water and N loss in vegetable production systems.
Going or Returning to Nature? Wild Vegetable Uses in the Foraging-Centered Restaurants of Lombardy, Northern Italy
Authors: Sulaiman Naji , Zocchi Dauro M. , Bonafede Sara , Nanni Chiara , Sõukand Renata , Pieroni Andrea
Published: August 2024

Wild vegetables (WVs) have been an essential source of human nutrition since ancient times. Foraging is a millennia-old practice that has gained more attention recently and is becoming fashionable, especially in restaurants in urban areas, as they attract customers who see WVs as an innovative sensory element and specialty food. Some cooks have used very few WVs for decades, but most chefs have only recently introduced them in their modern restaurants. Our study aims to have a deeper understanding of the diversity of WVs used by restaurants in the Lombardy region in Northern Italy and to know how they are introduced onto different menus, as well as the source of knowledge and the innovation paths related to the use/introduction of WVs in the selected sample of restaurants. Semi-structured interviews were conducted with 15 restaurant managers, chefs, and their professional foragers in the Lombardy region in Northern Italy in 2022; fifty-four wild plant taxa were recorded to be used in the considered restaurants. The collected data were analyzed to understand the current situation and the potential developments of this practice by exploring the reasons/motivations that underpin the inclusion of WVs in restaurants. A broad spectrum of restaurants was considered to evaluate the potential differences in handling and sourcing these ingredients. The results demonstrated that this trend has mainly been driven by attempts to revitalize traditional cuisines and to generate a positive impact on health, but the actual culinary preparations based upon WVs are often original and remarkably diverge from the Italian food ethnobotanical heritage. Moreover, concerns related to the environmental sustainability of these practices have been addressed.
Antioxidative and Metabolic Responses in Canola: Strategies with Wood Distillate and Sugarcane Bagasse Ash for Improved Growth under Abiotic Stress
Authors: Hafez Emad M. , Gao Yan , Alharbi Khadiga , Chen Wei , Elhawat Nevien , Alshaal Tarek , Osman Hany S.
Published: August 2024

In the context of increasing agricultural challenges posed by soil salinity and drought stress, the main importance of the present study was to evaluate some novel treatments for improving canola productivity and resilience by applying wood distillate (WD) in combination with bagasse ash (SBA). A two-year field experiment using a split plot design was conducted and evaluated several physiological and biochemical parameters under different irrigation regimes conducted at 80% and 50% field capacity. While there were considerable moderation effects of SBA and WD on soil salinity, expressed as exchangeable sodium percentage (ESP), under both well-irrigated and drought conditions, more importantly, the ESP was reduced to 31% under drought stress with combined WD and SBA applications over any single factor. WD and SBA treatments of canola leaves showed reduced Na content with increased K levels, and the plants maintained physiological attributes—chlorophyll content, stomatal conductance, and relative water content—to the level of controls of well-irrigation. Besides, they significantly alleviated oxidative stress by decreasing the hydrogen peroxide (H2O2), malondialdehyde (MDA), and electrolyte leakage (EL) levels and increasing the activities of antioxidant enzymes like superoxide dismutase (SOD) and ascorbate peroxidase (APX). Nonenzymatic antioxidants such as total soluble sugars (TSS), total soluble proteins (TSP), total phenolic content (TPC), and total flavonoid content (TFC) were significantly increased under stress conditions with a special accent on combined treatment, whereas the levels of proline and GB that increased in alignment with drought reduced under the combined application. Various growth parameters of plants like plant height, number of branches, and siliques per plant were significantly improved with WD and SBA under drought stress. Principal component analysis (PCA) and Pearson correlation further confirmed the relationships among these parameters and thus underpinned that WD and SBA can evoke a synergistic effect to enhance growth promotion and stress tolerance in canola. This, therefore, infers that the combined application of WD and SBA can be key, offering very high potential as viable options to better canola productivity under adverse environmental conditions.