bluuebunny/crossref_metadata_2025 huggingface dataset:
Re-Visiting Maximal Heart Rate Prediction Using Cross-Validation in Population Aged 7–55 Years
Authors: Park Jeong-Hui , Jung Hyun Chul , Jung Yeon-Sung , Song Jong-Kook , Lee Jung-Min
Published: July 2022

The primary purpose of the present study was to re-visit HRmax prediction by two commonly used equations (i.e., Fox′s and Tanaka′s equation) compared to the direct measured HRmax using the large sample size of Asians. The second aim of the study was to focus on suggesting new equations for the Asian population by separating gender and specific age groups. A total of 672 participants aged from 7 to 55 years were recruited for the study (male: 280 and female: 392), and the maximal graded exercise test with Bruce protocol was used to measure HRmax. All data obtained from the study were analyzed by SPSS 25.0. Additionally, three statistical analysis methods (i.e., Mean Absolute Percent Errors (MAPE), Bland–Altman plots, and equivalence testing) were utilized to confirm the consistency between the measured HRmax and the two prediction equations. The main finding was that two equations showed significant differences in predicting the HRmax of Korean aged from 7 to 55 years. The outcome of children aged from 7 to 14 was a different fit in the agreement compared to other age groups. Fox′s equation had the best fit in the average of the difference closer to zero and completely included within the equivalence zone, but females over 15 years old revealed higher errors than males in the values calculated by the two equations compared to the direct measured HRmax. Consequently, the study demonstrated that both equations tended to overestimate the HRmax for males and females over 15 years old, and the two universal equations were not suitable to predict the HRmax of Koreans except for children aged from 7 to 14 years. The new HRmax prediction equations suggested in this study will more accurately predict the HRmax of Asians, and additional analyses should be examined the cross-validity of the developed HRmax equation by age and gender in the future study.
Hourly Seamless Surface O3 Estimates by Integrating the Chemical Transport and Machine Learning Models in the Beijing-Tianjin-Hebei Region
Authors: Xue Wenhao , Zhang Jing , Hu Xiaomin , Yang Zhe , Wei Jing
Published: July 2022

Surface ozone (O3) is an important atmospheric trace gas, posing an enormous threat to ecological security and human health. Currently, the core objective of air pollution control in China is to realize the joint treatment of fine particulate matter (PM2.5) and O3. However, high-accuracy near-surface O3 maps remain lacking. Therefore, we established a new model to determine the full-coverage hourly O3 concentration with the WRF-Chem and random forest (RF) models combined with anthropogenic emission data and meteorological datasets. Based on this method, choosing the Beijing-Tianjin-Hebei (BTH) region in 2018 as an example, full-coverage hourly O3 maps were generated at a horizontal resolution of 9 km. The performance evaluation results indicated that the new model is reliable with a sample (station)-based 10-fold cross-validation (10-CV) R2 value of 0.94 (0.90) and root mean square error (RMSE) of 14.58 (19.18) µg m−3. In addition, the estimated O3 concentration is accurately determined at varying temporal scales with sample-based 10-CV R2 values of 0.96, 0.98 and 0.98 at the daily, monthly, and seasonal scales, respectively, which is highly superior to traditional derivation algorithms and other techniques in previous studies. An initial increase and subsequent decrease, which constitute the diurnal variation in the O3 concentration associated with temperature and solar radiation variations, were captured. The highest concentration reached approximately 112.73 ± 9.65 μg m−3 at 15:00 local time (1500 LT) in the BTH region. Summertime O3 posed a high pollution risk across the whole BTH region, especially in southern cities, and the pollution duration accounted for more than 50% of the summer season. Additionally, 43 and two days exhibited light and moderate O3 pollution, respectively, across the BTH region in 2018. Overall, the new method can be beneficial for near-surface O3 estimation with a high spatiotemporal resolution, which can be valuable for research in related fields.
COVID-19 and Digital Health: Evolution, Perspectives and Opportunities
Authors: Dettori Marco , Castiglia Paolo
Published: July 2022

Health Communication is key to establishing an empathic relationship between health professionals and their patients. Indeed, the ability to motivate and reassure the patient often determines the success of the therapies proposed. In the past, the relationship between health professionals and patients was centred on treatment and assistance, and health information came in the form of education campaigns based on signage (large posters, etc.). Subsequently, social and cultural changes gave rise to new ways of communicating science: from signage to magazines and television programmes devoted to health issues, through the use of social networks. In particular, fast and cheap access to the Internet and the vast number of app users have made the Web an effective communication tool. Given the potential of technology in the information-seeking process, the use of online channels by health institutions is a valuable tool for divulging medical and scientific knowledge. As a consequence, prompted by the need to provide fast and reliable information to the population, public institutions have adopted such innovative methods as the use of Information and Communication Technologies (ICTs) to convey health-related content. This practice, known as digital health or e-health, provides healthcare information using digital tools (e.g., Websites and social networks), delivered in an easy-to-understand language in order to reach various population groups and bring about better health conditions for all, hence the importance of acquiring and reinforcing communication skills in the healthcare field, where correct and effective communication immediately translates into a benefit for the professionals themselves and for their patients.
Attitudes and Opinions of Parents towards Water-Only Drink Policy at Junior Triathlon Events
Authors: Devlin Brooke , Staley Kiera , Trakman Gina , Forsyth Adrienne , Nicholson Matthew , Cosgriff Grant , Chisholm Melanie , Belski Regina
Published: July 2022

Rates of childhood obesity within Australia continue to rise, with consumption of sugar-sweetened beverages one contributing factor. Community sport provides an opportunity to implement policies promoting water as the beverage of choice. However, the attitudes of parents toward a water-only policy are not known. This cross-sectional study aimed to investigate parents’ opinions towards beverage consumption and a water-only policy. Data were collected from participants (n = 159) using an investigator-designed questionnaire, administered using iPads, at a junior Triathlon Victoria event. Water was the most popular beverage provided before (75%), during (85%) and after (61%) sport. Parents were more likely to provide sports drinks to children older than 14 years (27%). Three-quarters (77%) of parents reported having received no information regarding hydration requirements. Parents rated the importance of hydration prior to, during and after a triathlon as high (9.08 ± 1.2, 8.76 ± 1.3 and 9.30 ± 0.4 out of 10, respectively). Parents were supportive of a water-only policy at all junior triathlon events and all junior sporting events (7.94 ± 1.3 and 7.86 ± 1.9, respectively). There was less support for a water-only policy for adult triathlons (6.40 ± 3.1). A water-only drink policy at junior sport is viewed positively by parents. This warrants further research and policy development to facilitate behaviour change.
Care Recommendations for the Chronic Risk of COVID-19: Nursing Intervention for Behaviour Changes
Authors: González Aguña Alexandra , Fernández Batalla Marta , Gonzalo de Diego Blanca , Jiménez Rodríguez María Lourdes , Martínez Muñoz María Lourdes , Santamaría García José María
Published: July 2022

The COVID-19 pandemic is a challenge for health systems. The absence of prior evidence makes it difficult to disseminate consensual care recommendations. However, lifestyle adaptation is key to controlling the pandemic. In light of this, nursing has its own model and language that allow these recommendations to be combined from global and person-centred perspectives. The purpose of the study is to design a population-oriented care recommendation guide for COVID-19. The methodology uses a group of experts who provide classified recommendations according to Gordon’s functional patterns, after which a technical team unifies them and returns them for validation through the content validity index (CVI). The experts send 1178 records representing 624 recommendations, which are unified into 258. In total, 246 recommendations (95.35%) are validated, 170 (65.89%) obtain high validation with CVI > 0.80, and 12 (4.65%) are not validated by CVI < 0.50. The mean CVI per pattern is 0.84 (0.70–0.93). These recommendations provide a general framework from a nursing care perspective. Each professional can use this guide to adapt the recommendations to each individual or community and thus measure the health impact. In the future, this guideline could be updated as more evidence becomes available.
Gender Diversity in Leadership: A Bibliometric Analysis and Future Research Directions
Authors: Vieira Elisabete , Madaleno Mara , Lobão Júlio
Published: July 2022

Gender diversity in management has become of increased interest to academics and researchers in the last decades since women and men have different personal and psychological characteristics that affect their decision-making process in management actions and leadership. A female presence has been under-represented on companies’ boards of directors, which affects management decisions and leadership style. This article aims to contribute to the analysis of gender diversity and leadership state of knowledge, conducting a bibliometric review of the existing literature. In this context, we analyze the evolutional research studies published in the Scopus digital library, considering a period of five years, from 2017 to 2021. We focus our analysis on the top 24 cited articles after a wider review of articles published relating to the explored topic (402 articles). Considering the bibliometric analysis done, we then present scrutiny of the state of knowledge on this subject, a summary of the existing gaps, and suggestions for future research.
A Deep Learning Approach to Dynamic Interbank Network Link Prediction
Authors: Zhang Haici
Published: July 2022

Lehman Brothers’ failure in 2008 demonstrated the importance of understanding interconnectedness in interbank networks. The interbank market plays a significant role in facilitating market liquidity and providing short-term funding for each other to smooth liquidity shortages. Knowing the trading relationship could also help understand risk contagion among banks. Therefore, future lending relationship prediction is important to understand the dynamic evolution of interbank networks. To achieve the goal, we apply a deep learning framework model of interbank lending to an electronic trading interbank network for temporal trading relationship prediction. There are two important components of the model, which are the Graph convolutional network (GCN) and the Long short-term memory (LSTM) model. The GCN and LSTM components together capture the spatial–temporal information of the dynamic network snapshots. Compared with the Discrete autoregressive model and Dynamic latent space model, our proposed model achieves better performance in both the precrisis and the crisis period.
Transcriptomics Advancement in the Complex Response of Plants to Viroid Infection
Authors: Joubert Melissa , van den Berg Noëlani , Theron Jacques , Swart Velushka
Published: July 2022

Viroids are the smallest plant pathogens, consisting of a single-stranded circular RNA of less than 500 ribonucleotides in length. Despite their noncoding nature, viroids elicit disease symptoms in many economically important plant hosts, and are, thus, a class of pathogens of great interest. How these viroids establish disease within host plants, however, is not yet fully understood. Recent transcriptomic studies have revealed that viroid infection influences the expression of genes in several pathways and processes in plants, including defence responses, phytohormone signalling, cell wall modification, photosynthesis, secondary metabolism, transport, gene expression and protein modification. There is much debate about whether affected pathways signify a plant response to viroid infection, or are associated with the appearance of disease symptoms in these interactions. In this review, we consolidate the findings of viroid–host transcriptome studies to provide an overview of trends observed in the data. When considered together, changes in the gene expression of different hosts upon viroid infection reveal commonalities and differences in diverse interactions. Here, we discuss whether trends in host gene expression can be correlated to plant defence or disease development during viroid infection, and highlight avenues for future research in this field.
Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli
Authors: Köppl Christoph , Lingg Nico , Fischer Andreas , Kröß Christina , Loibl Julian , Buchinger Wolfgang , Schneider Rainer , Jungbauer Alois , Striedner Gerald , Cserjan-Puschmann Monika
Published: July 2022

Fusion protein technologies to facilitate soluble expression, detection, or subsequent affinity purification in Escherichia coli are widely used but may also be associated with negative consequences. Although commonly employed solubility tags have a positive influence on titers, their large molecular mass inherently results in stochiometric losses of product yield. Furthermore, the introduction of affinity tags, especially the polyhistidine tag, has been associated with undesirable changes in expression levels. Fusion tags are also known to influence the functionality of the protein of interest due to conformational changes. Therefore, particularly for biopharmaceutical applications, the removal of the fusion tag is a requirement to ensure the safety and efficacy of the therapeutic protein. The design of suitable fusion tags enabling the efficient manufacturing of the recombinant protein remains a challenge. Here, we evaluated several N-terminal fusion tag combinations and their influence on product titer and cell growth to find an ideal design for a generic fusion tag. For enhancing soluble expression, a negatively charged peptide tag derived from the T7 bacteriophage was combined with affinity tags and a caspase-2 cleavage site applicable for CASPase-based fusiON (CASPON) platform technology. The effects of each combinatorial tag element were investigated in an integrated manner using human fibroblast growth factor 2 as a model protein in fed-batch lab-scale bioreactor cultivations. To confirm the generic applicability for manufacturing, seven additional pharmaceutically relevant proteins were produced using the best performing tag of this study, named CASPON-tag, and tag removal was demonstrated.
Sexual Dimorphism in Adipose-Hypothalamic Crosstalk and the Contribution of Aryl Hydrocarbon Receptor to Regulate Energy Homeostasis
Authors: Haque Nazmul , Tischkau Shelley A.
Published: July 2022

There are fundamental sex differences in the regulation of energy homeostasis. Better understanding of the underlying mechanisms of energy balance that account for this asymmetry will assist in developing sex-specific therapies for sexually dimorphic diseases such as obesity. Multiple organs, including the hypothalamus and adipose tissue, play vital roles in the regulation of energy homeostasis, which are regulated differently in males and females. Various neuronal populations, particularly within the hypothalamus, such as arcuate nucleus (ARC), can sense nutrient content of the body by the help of peripheral hormones such leptin, derived from adipocytes, to regulate energy homeostasis. This review summarizes how adipose tissue crosstalk with homeostatic network control systems in the brain, which includes energy regulatory regions and the hypothalamic–pituitary axis, contribute to energy regulation in a sex-specific manner. Moreover, development of obesity is contingent upon diet and environmental factors. Substances from diet and environmental contaminants can exert insidious effects on energy metabolism, acting peripherally through the aryl hydrocarbon receptor (AhR). Developmental AhR activation can impart permanent alterations of neuronal development that can manifest a number of sex-specific physiological changes, which sometimes become evident only in adulthood. AhR is currently being investigated as a potential target for treating obesity. The consensus is that impaired function of the receptor protects from obesity in mice. AhR also modulates sex steroid receptors, and hence, one of the objectives of this review is to explain why investigating sex differences while examining this receptor is crucial. Overall, this review summarizes sex differences in the regulation of energy homeostasis imparted by the adipose–hypothalamic axis and examines how this axis can be affected by xenobiotics that signal through AhR.
Predicting Antigenic Peptides from Rocio Virus NS1 Protein for Immunodiagnostic Testing Using Immunoinformatics and Molecular Dynamics Simulation
Authors: Saivish Marielena Vogel , Menezes Gabriela de Lima , Costa Vivaldo Gomes da , Silva Gislaine Celestino Dutra da , Marques Rafael Elias , Nogueira Maurício Lacerda , Silva Roosevelt Alves Da
Published: July 2022

The mosquito-borne disease caused by the Rocio virus is a neglected threat, and new immune inputs for serological testing are urgently required for diagnosis in low-resource settings and epidemiological surveillance. We used in silico approaches to identify a specific antigenic peptide (p_ROCV2) in the NS1 protein of the Rocio virus that was theoretically predicted to be stable and exposed on its surface, where it demonstrated key properties allowing it to interact with antibodies. These findings related to the molecular dynamics of this peptide provide important insights for advancing diagnostic platforms and investigating therapeutic alternatives.
Gold Nanoparticles Contact with Cancer Cell: A Brief Update
Authors: Bloise Nora , Strada Silvia , Dacarro Giacomo , Visai Livia
Published: July 2022

The fine-tuning of the physicochemical properties of gold nanoparticles has facilitated the rapid development of multifunctional gold-based nanomaterials with diagnostic, therapeutic, and therapeutic applications. Work on gold nanoparticles is increasingly focusing on their cancer application. This review provides a summary of the main biological effects exerted by gold nanoparticles on cancer cells and highlights some critical factors involved in the interaction process (protein corona, tumor microenvironment, surface functionalization). The review also contains a brief discussion of the application of gold nanoparticles in target discovery.
Squaramide-Tethered Sulfonamides and Coumarins: Synthesis, Inhibition of Tumor-Associated CAs IX and XII and Docking Simulations
Authors: Arrighi Giulia , Puerta Adrián , Petrini Andrea , Hicke Francisco J. , Nocentini Alessio , Fernandes Miguel X. , Padrón José M. , Supuran Claudiu T. , Fernández-Bolaños José G. , López Óscar
Published: July 2022

(1) Background: carbonic anhydrases (CAs) are attractive targets for the development of new anticancer therapies; in particular, CAs IX and XII isoforms are overexpressed in numerous tumors. (2) Methods: following the tail approach, we have appended a hydrophobic aromatic tail to a pharmacophore responsible for the CA inhibition (aryl sulfonamide, coumarin). As a linker, we have used squaramides, featured with strong hydrogen bond acceptor and donor capacities. (3) Results: Starting from easily accessible dimethyl squarate, the title compounds were successfully obtained as crystalline solids, avoiding the use of chromatographic purifications. Interesting and valuable SARs could be obtained upon modification of the length of the hydrocarbon chain, position of the sulfonamido moiety, distance of the aryl sulfonamide scaffold to the squaramide, stereoelectronic effects on the aromatic ring, as well as the number and type of substituents on C-3 and C-4 positions of the coumarin. (4) Conclusions: For sulfonamides, the best profile was achieved for the m-substituted derivative 11 (Ki = 29.4, 9.15 nM, CA IX and XII, respectively), with improved selectivity compared to acetazolamide, a standard drug. Coumarin derivatives afforded an outstanding selectivity (Ki > 10,000 nM for CA I, II); the lead compound (16c) was a strong CA IX and XII inhibitor (Ki = 19.2, 7.23 nM, respectively). Docking simulations revealed the key ligand-enzyme interactions.
Synthesis of Novel Aminothiazole Derivatives as Promising Antiviral, Antioxidant and Antibacterial Candidates
Authors: Minickaitė Rūta , Grybaitė Birutė , Vaickelionienė Rita , Kavaliauskas Povilas , Petraitis Vidmantas , Petraitienė Rūta , Tumosienė Ingrida , Jonuškienė Ilona , Mickevičius Vytautas
Published: July 2022

It is well-known that thiazole derivatives are usually found in lead structures, which demonstrate a wide range of pharmacological effects. The aim of this research was to explore the antiviral, antioxidant, and antibacterial activities of novel, substituted thiazole compounds and to find potential agents that could have biological activities in one single biomolecule. A series of novel aminothiazoles were synthesized, and their biological activity was characterized. The obtained results were compared with those of the standard antiviral, antioxidant, antibacterial and anticancer agents. The compound bearing 4-cianophenyl substituent in the thiazole ring demonstrated the highest cytotoxic properties by decreasing the A549 viability to 87.2%. The compound bearing 4-trifluoromethylphenyl substituent in the thiazole ring showed significant antiviral activity against the PR8 influenza A strain, which was comparable to the oseltamivir and amantadine. Novel compounds with 4-chlorophenyl, 4-trifluoromethylphenyl, phenyl, 4-fluorophenyl, and 4-cianophenyl substituents in the thiazole ring demonstrated antioxidant activity by DPPH, reducing power, FRAP methods, and antibacterial activity against Escherichia coli and Bacillus subtilis bacteria. These data demonstrate that substituted aminothiazole derivatives are promising scaffolds for further optimization and development of new compounds with potential influenza A-targeted antiviral activity. Study results could demonstrate that structure optimization of novel aminothiazole compounds may be useful in the prevention of reactive oxygen species and developing new specifically targeted antioxidant and antibacterial agents.
Strategies for Controlling the Spatial Orientation of Single Molecules Tethered on DNA Origami Templates Physisorbed on Glass Substrates: Intercalation and Stretching
Authors: Cervantes-Salguero Keitel , Biaggne Austin , Youngsman John M. , Ward Brett M. , Kim Young C. , Li Lan , Hall John A. , Knowlton William B. , Graugnard Elton , Kuang Wan
Published: July 2022

Nanoarchitectural control of matter is crucial for next-generation technologies. DNA origami templates are harnessed to accurately position single molecules; however, direct single molecule evidence is lacking regarding how well DNA origami can control the orientation of such molecules in three-dimensional space, as well as the factors affecting control. Here, we present two strategies for controlling the polar (θ) and in-plane azimuthal (ϕ) angular orientations of cyanine Cy5 single molecules tethered on rationally-designed DNA origami templates that are physically adsorbed (physisorbed) on glass substrates. By using dipolar imaging to evaluate Cy5′s orientation and super-resolution microscopy, the absolute spatial orientation of Cy5 is calculated relative to the DNA template. The sequence-dependent partial intercalation of Cy5 is discovered and supported theoretically using density functional theory and molecular dynamics simulations, and it is harnessed as our first strategy to achieve θ control for a full revolution with dispersion as small as ±4.5°. In our second strategy, ϕ control is achieved by mechanically stretching the Cy5 from its two tethers, being the dispersion ±10.3° for full stretching. These results can in principle be applied to any single molecule, expanding in this way the capabilities of DNA as a functional templating material for single-molecule orientation control. The experimental and modeling insights provided herein will help engineer similar self-assembling molecular systems based on polymers, such as RNA and proteins.
Characterization of Endothelial Progenitor Cell: Past, Present, and Future
Authors: Salybekov Amankeldi A. , Kobayashi Shuzo , Asahara Takayuki
Published: July 2022

Endothelial progenitor cells (EPCs) are currently being studied as candidate cell sources for revascularization strategies. Despite these promising results, widespread clinical acceptance of EPCs for clinical therapies remains hampered by several challenges. The challenges and issues surrounding the use of EPCs and the current paradigm being developed to improve the harvest efficiency and functionality of EPCs for application in regenerative medicine are discussed. It has been observed that controversies have emerged regarding the isolation techniques and classification and origin of EPCs. This manuscript attempts to highlight the concept of EPCs in a sequential manner, from the initial discovery to the present (origin, sources of EPCs, isolation, and identification techniques). Human and murine EPC marker diversity is also discussed. Additionally, this manuscript is aimed at summarizing our current and future prospects regarding the crosstalk of EPCs with the biology of hematopoietic cells and culture techniques in the context of regeneration-associated cells (RACs).
Deficiency in Inactive Rhomboid Protein2 (iRhom2) Alleviates Alcoholic Liver Fibrosis by Suppressing Inflammation and Oxidative Stress
Authors: Liu Yangwenshu , Kuang Qin , Dai Xianling , Zhan Minxia , Zhou Li , Zhu Liancai , Wang Bochu
Published: July 2022

Chronic alcohol exposure can lead to liver pathology relating to inflammation and oxidative stress, which are two of the major factors in the incidence of liver fibrosis and even liver cancer. The underlying molecular mechanisms regarding hepatic lesions associated with alcohol are not fully understood. Considering that the recently identified iRhom2 is a key pathogenic mediator of inflammation, we performed in vitro and in vivo experiments to explore its regulatory role in alcohol-induced liver fibrosis. We found that iRhom2 knockout significantly inhibited alcohol-induced inflammatory responses in vitro, including elevated expressions of inflammatory cytokines (IL-1β, IL-6, IL-18, and TNF-α) and genes associated with inflammatory signaling pathways, such as TACE (tumor necrosis factor-alpha converting enzyme), TNFR1 (tumor necrosis factor receptor 1), and TNFR2, as well as the activation of NF-κB. The in vivo results confirmed that long-term alcohol exposure leads to hepatocyte damage and fibrous accumulation. In this pathological process, the expression of iRhom2 is promoted to activate the TACE/NF-κB signaling pathway, leading to inflammatory responses. Furthermore, the deletion of iRhom2 blocks the TACE/NF-κB signaling pathway and reduces liver damage and fibrosis caused by alcohol. Additionally, the activation of the JNK/Nrf2/HO-1 signaling pathway caused by alcohol exposure was also noted in vitro and in vivo. In the same way, knockout or deleting iRhom2 blocked the JNK/Nrf2/HO-1 signaling pathway to regulate the oxidative stress. Therefore, we contend that iRhom2 is a key regulator that promotes inflammatory responses and regulates oxidative stress in alcoholic liver fibrosis lesions. We posit that iRhom2 is potentially a new therapeutic target for alcoholic liver fibrosis.
Influence of Association on Binding of Disaccharides to YKL-39 and hHyal-1 Enzymes
Authors: Krzemińska Agnieszka , Sánchez-Aparicio José-Emilio , Maréchal Jean-Didier , Paneth Agata , Paneth Piotr
Published: July 2022

Disaccharide complexes have been shown experimentally to be useful for drug delivery or as an antifouling surface biofilm, and are promising drug-encapsulation and delivery candidates. Although such complexes are intended for medical applications, to date no studies at the molecular level have been devoted to the influence of complexation on the enzymatic decomposition of polysaccharides. A theoretical approach to this problem has been hampered by the lack of a suitable computational tool for binding such non-covalent complexes to enzymes. Herein, we combine quantum-mechanical calculations of disaccharides complexes with a nonstandard docking GaudiMM engine that can perform such a task. Our results on four different complexes show that they are mostly stabilized by electrostatic interactions and hydrogen bonds. This strong non-covalent stabilization demonstrates the studied complexes are some excellent candidates for self-assembly smart materials, useful for drug encapsulation and delivery. Their advantage lies also in their biocompatible and biodegradable character.
Lipophilic Bioactive Compounds Transported in Triglyceride-Rich Lipoproteins Modulate Microglial Inflammatory Response
Authors: Espinosa Juan M. , Castellano Jose M. , Garcia-Rodriguez Silvia , Quintero-Flórez Angélica , Carrasquilla Natalia , Perona Javier S.
Published: July 2022

Microglial cells can contribute to Alzheimer’s disease by triggering an inflammatory response that leads to neuronal death. In addition, the presence of amyloid-β in the brain is consistent with alterations in the blood–brain barrier integrity and triglyceride-rich lipoproteins (TRL) permeation. In the present work, we used lab-made TRL as carriers of lipophilic bioactive compounds that are commonly present in dietary oils, namely oleanolic acid (OA), α-tocopherol (AT) and β-sitosterol (BS), to assess their ability to modulate the inflammatory response of microglial BV-2 cells. We show that treatment with lab-made TRL increases the release and gene-expression of IL-1β, IL-6, and TNF-α, as well as NO and iNOS in microglia. On the other hand, TRL revealed bioactive compounds α-tocopherol and β-sitosterol as suitable carriers for oleanolic acid. The inclusion of these biomolecules in TRL reduced the release of proinflammatory cytokines. The inclusion of these biomolecules in TRL reduced the release of proinflammatory cytokines. AT reduced IL-6 release by 72%, OA reduced TNF-α release by approximately 50%, and all three biomolecules together (M) reduced IL-1β release by 35% and TNF-α release by more than 70%. In addition, NO generation was reduced, with the inclusion of OA by 45%, BS by 80% and the presence of M by 88%. Finally, a recovery of the basal glutathione content was observed with the inclusion of OA and M in the TRL. Our results open the way to exploiting the neuro-pharmacological potential of these lipophilic bioactive compounds through their delivery to the brain as part of TRL.
Of Mouse and Man: Cross-Species Characterization of Hypertensive Cardiac Remodeling
Authors: Cooper Susanna T. E. , Westaby Joseph D. , Haines Zoe H. R. , Malone Giles O. , Sheppard Mary N. , Meijles Daniel N.
Published: July 2022

Hypertension is a major public health concern and poses a significant risk for sudden cardiac death (SCD). However, the characterisation of human tissues tends to be macroscopic, with little appreciation for the quantification of the pathological remodelling responsible for the advancement of the disease. While the components of hypertensive remodelling are well established, the timeline and comparative quantification of pathological changes in hypertension have not been shown before. Here, we sought to identify the phasing of cardiac remodelling with hypertension using post-mortem tissue from SCD patients with early and advanced hypertensive heart disease (HHD). In order to study and quantify the progression of phenotypic changes, human specimens were contrasted to a well-described angiotensin-II-mediated hypertensive mouse model. While cardiomyocyte hypertrophy is an early adaptive response in the mouse that stabilises in established hypertension and declines as the disease progresses, this finding did not translate to the human setting. In contrast, optimising fibrosis quantification methods and applying them to each setting identified perivascular fibrosis as the prevailing possible cause for overall disease progression. Indeed, assessing myocardial inflammation highlights CD45+ inflammatory cell infiltration that precedes fibrosis and is an early-phase event in response to elevated arterial pressures that may underscore perivascular remodelling. Along with aetiology insight, we highlight cross-species comparison for quantification of cardiac remodelling in human hypertension. As such, this platform could assist with the development of therapies specific to the disease phase rather than targeting global components of hypertension, such as blood pressure lowering.
Visible-Light-Driven Antimicrobial Activity and Mechanism of Polydopamine-Reduced Graphene Oxide/BiVO4 Composite
Authors: Li Biyun , Gao Xiaoxiao , Qu Jiangang , Xiong Feng , Xuan Hongyun , Jin Yan , Yuan Huihua
Published: July 2022

In this study, a photocatalytic antibacterial composite of polydopamine-reduced graphene oxide (PDA-rGO)/BiVO4 is prepared by a hydrothermal self-polymerization reduction method. Its morphology and physicochemical properties are characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier-transform infrared (FT-IR), and X-ray diffraction (XRD). The results indicate that BiVO4 particles are evenly distributed on the rGO surface. Escherichia coli (E. coli) MG1655 is selected as the model bacteria, and its antibacterial performance is tested by flat colony counting and the MTT method under light irradiation. PDA-rGO/BiVO4 inhibits the growth of E. coli under both light and dark conditions, and light significantly enhances the bacteriostasis of PDA-rGO/BiVO4. A combination of BiVO4 with PDA-rGO is confirmed by the above characterization methods as improving the photothermal performance under visible light irradiation. The composite possesses enhanced photocatalytic antibacterial activity. Additionally, the photocatalytic antibacterial mechanism is investigated via the morphology changes in the SEM images of MG1655 bacteria, 2′,7′-dichlorofluorescein diacetate (DCFH-DA), the fluorescence detection of the reactive oxygen species (ROS), and gene expression. These results show that PDA-rGO/BiVO4 can produce more ROS and lead to bacterial death. Subsequently, the q-PCR results show that the transmembrane transport of bacteria is blocked and the respiratory chain is inhibited. This study may provide an important strategy for expanding the application of BiVO4 in biomedicine and studying the photocatalytic antibacterial mechanism.
Risk Stratification of Patients with Pulmonary Arterial Hypertension: The Role of Echocardiography
Authors: Mercurio Valentina , Hassan Hussein J. , Naranjo Mario , Cuomo Alessandra , Mazurek Jeremy A. , Forfia Paul R. , Balasubramanian Aparna , Simpson Catherine E. , Damico Rachel L. , Kolb Todd M. , Mathai Stephen C. , Hsu Steven , Mukherjee Monica , Hassoun Paul M.
Published: July 2022

Background: Given the morbidity and mortality associated with pulmonary arterial hypertension (PAH), risk stratification approaches that guide therapeutic management have been previously employed. However, most patients remain in the intermediate-risk category despite initial therapy. Herein, we sought to determine whether echocardiographic parameters could improve the risk stratification of intermediate-risk patients. Methods: Prevalent PAH patients previously enrolled in observational studies at 3 pulmonary hypertension centers were included in this study. A validated PAH risk stratification approach was used to stratify patients into low-, intermediate-, and high-risk groups. Right ventricular echocardiographic parameters were used to further stratify intermediate-risk patients into intermediate-low- and intermediate-high-risk groups based on transplant-free survival. Results: From a total of 146 patients included in our study, 38 patients died over a median follow-up of 2.5 years. Patients with intermediate-/high-risk had worse echocardiographic parameters. Tricuspid annular plane systolic excursion (TAPSE) and the degree of tricuspid regurgitation (TR) were highly associated with survival (p < 0.01, p = 0.04, respectively) and were subsequently used to further stratify intermediate-risk patients. Among intermediate-risk patients, survival was worse for patients with TAPSE < 19 mm compared to those with TAPSE ≥ 19 mm (estimated one-year survival 74% vs. 96%, p < 0.01) and for patients with moderate/severe TR compared to those with no/trace/mild TR (estimated one-year survival 70% vs. 93%, p < 0.01). Furthermore, among intermediate-risk patients, those with both TAPSE < 19 mm and moderate/severe TR had an estimated one-year survival (56%) similar to that of high-risk patients (56%), and those with both TAPSE ≥ 19 mm and no/trace/mild TR had an estimated one-year survival (97%) similar to that of low-risk patients (95%). Conclusions: Echocardiography, a routinely performed, non-invasive imaging modality, plays a pivotal role in discriminating distinct survival phenotypes among prevalent intermediate-risk PAH patients using TAPSE and degree of TR. This can potentially help guide subsequent therapy.
Cost, Energy Efficiency and Carbon Footprint Analysis of Hybrid Light-Weight Bulk Carrier
Authors: Palomba Giulia , Scattareggia Marchese Simone , Crupi Vincenzo , Garbatov Yordan
Published: July 2022

Integrating innovative solutions for ship design has always been a great challenge for the maritime sector due to complex design and construction processes. With this scenario in mind, the objective of this study was to develop a procedure to evaluate the potential benefits arising from the integration of innovative light-weight structures in ship hull structural design. To achieve such an objective, a hybrid light-weight ship hull structural design solution, in which aluminium honeycomb sandwich panels were used to build the conventional steel inner side shell of the cargo holds, was adopted for a bulk carrier. The authors of this study used a multiple criteria decision-making approach. An optimal ship hull structural design solution was identified based on capital cost, voyage cost, annual cost, energy efficiency design index, dismantling–reselling cost, cargo transportation, energy consumption and carbon footprint. The optimal solution, identified with the multiple criteria decision-making approach, improved the ship’s efficiency and costs by combining the hybrid structural design with efficient cargo transportation. In addition, using recycled aluminium was found to be a promising strategy to reduce the energy consumption and carbon footprint related to the shipbuilding process.
Postantifungal Effect of Antifungal Drugs against Candida: What Do We Know and How Can We Apply This Knowledge in the Clinical Setting?
Authors: Jauregizar Nerea , Quindós Guillermo , Gil-Alonso Sandra , Suárez Elena , Sevillano Elena , Eraso Elena
Published: July 2022

The study of the pharmacological properties of an antifungal agent integrates the drug pharmacokinetics, the fungal growth inhibition, the fungicidal effect and the postantifungal activity, laying the basis to guide optimal dosing regimen selection. The current manuscript reviews concepts regarding the postantifungal effect (PAFE) of the main classes of drugs used to treat Candida infections or candidiasis. The existence of PAFE and its magnitude are highly dependent on both the fungal species and the class of the antifungal agent. Therefore, the aim of this article was to compile the information described in the literature concerning the PAFE of polyenes, azoles and echinocandins against the Candida species of medical interest. In addition, the mechanisms involved in these phenomena, methods of study, and finally, the clinical applicability of these studies relating to the design of dosing regimens were reviewed and discussed. Additionally, different factors that could determine the variability in the PAFE were described. Most PAFE studies were conducted in vitro, and a scarcity of PAFE studies in animal models was observed. It can be stated that the echinocandins cause the most prolonged PAFE, followed by polyenes and azoles. In the case of the triazoles, it is worth noting the inconsistency found between in vitro and in vivo studies.
Reconstructing the Social Image of Older Women and Ageing: The Transformative Power of the Narrative Set in the Local Context
Authors: Sanvicen-Torné Paquita , Stončikaitė Ieva , Soldevila-Benet Anna , Molina-Luque Fidel
Published: July 2022

This case study reveals that age-related areas are the least desirable professional future options for many university students in social work degree programmes. One of the possible causes is the negative social labelling of older age, especially pronounced in respect of older women. Additionally, there is a poor and limited educational approach towards later life and growing older inside and outside the educational settings. This article focuses on the social construction of older age from gender and double theoretical perspectives. In particular, it centers on the pillars of education and profiguration. For educational and analytical purposes, these aspects are approached in the classroom setting from a critical perspective by using the in-depth reading of a book that is set in the local context, in particular, the city of Lleida (Spain). It presents the results of the content analysis and reflections written by 170 first-year university students taking a degree course in social work, and the outcomes of the subsequent classroom discussions with the author of the book. The study results show that better knowledge about the complexities of ageing and later life can lead to the reconstruction of the students’ viewpoints about older age, help foster critical thinking, and defy age-related stereotypes, beliefs, and prejudices.
An Evaluation of the Development Performance of Small County Towns and Its Influencing Factors: A Case Study of Small Towns in Jiangyin City in the Yangtze River Delta, China
Authors: Gong Xiao , Zhang Xiaolin , Tao Jieyi , Li Hongbo , Zhang Yunrui
Published: July 2022

Research on the development performance of small towns is critical for promoting their revitalization, advancing urbanization, and high-quality development and transformation for realizing urban–rural integration. We used the DPSIR-DEA model to study the spatiotemporal evolution process and characteristics of the development performance of 14 small towns within the administrative division of Jiangyin city from 2001 to 2019. We subsequently applied a geographical detector model to analyze the spatiotemporal heterogeneity of the factors influencing the development performance of small towns. The results showed that 2012 was a turning point in the overall development performance index of small towns in Jiangyin, revealing initially decreasing and then increasing trends. The development performance index values of different types of small towns evidenced three trends: a steady increase, a continuous decrease, and an initial decrease followed by an increase. During 2001–2019, the development performance of Jiangyin’s small towns reflected a spatial evolution pattern of complete dispersion → small agglomeration → large agglomeration. An optimal spatial pattern comprised an increase in the number of towns demonstrating a high development performance and a decrease in the number of towns with a low development performance. GDP per capita, industrial investments, and construction land density were key influencing factors of development performance, which was mainly driven by economic and social factors, with ecological factors having a relatively weak influence.
Land Use Improvement as the Drought Mitigation to Manage Climate Change in the Dodokan Watershed, Lombok, Indonesia
Authors: Nandini Ryke , Kusumandari Ambar
Published: July 2022

The Dodokan watershed is one of the priority watersheds on Lombok Island, which is a mainstay for providing water for the community but has a high vulnerability to drought. This condition causes disruption to the fulfillment of water needs, so it needs to be anticipated early with mitigation actions. Understanding the trend of drought is needed as a basis for developing mitigation measures, especially related to land use in the watershed. This study aims to analyze the hydro-meteorological drought trend that occurred from 2009 to 2020 and to find out the role of land use improvement in mitigating drought, which is predicted in the next 10 years. The Thornthwaite–Mather method was used for drought analysis, and its predictions for the next 10 years were determined by using trend analysis. Land use simulation using a spatial analysis was carried out as an effort to prepare for drought mitigation actions. The results show that the drought in the Dodokan Watershed has increased in the period between 2009–2020 and is predicted to continue to increase. Land use improvement by restoring the forest is a more appropriate mitigation method to overcome the drought than rearranging the agricultural land on slopes above 25%. The simulation results have succeeded in increasing the water surplus and reducing the water deficit in 2030 so that it can be used for drought mitigation in the Dodokan watershed.
What Does the Circular Household of the Future Look Like? An Expert-Based Exploration
Authors: Keesstra Saskia , Metze Tamara , Ofori Linda , Buizer Marleen , Visser Saskia
Published: July 2022

Circularity is a necessity for the future of our society but individual households often find it difficult to contribute to this transition. This paper presents possible future visions of circular (and climate-neutral) households, inside and outside the house, regarding their contributions to the circular society, and taking into account food, energy, waste, household devices, and recreation. We combined expert interviews and a literature review to (1) explore imaginable futures for circular households, and (2) make a qualitative evaluation of the inside- and outside-house influences of households on a climate-neutral and circular society. Interviewees were selected to represent different scientific backgrounds. The four household types were organized according to more local or global, and collective or individual, levels: (1) the Househood (centering around neighborhoods); (2) the HouseNet (connecting households); (3) the Sharing Household (sharing goods between households); and (4) the Designing Household (input from circular-by-design products). The analysis shows that households can become more circular by connecting developments in social, ecological, and technological systems, such as those in price dynamics, policies, or land-use design. However, barriers and limitations need attention, including: (1) public awareness and willingness to change; (2) economic models; (3) waste; and (4) social justice.