bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevention of spinal fusion post-operative wound infections in pediatric patients with scoliosis: a quality improvement initiative
Authors: Partridge Elizabeth , Blumberg Dean , Roberto Rolando F.
Published: January 2021

Purpose

Post-operative wound infections increase patient morbidity and mortality as well as the length of hospital stay, with a profound personal and institutional cost. The aim of this study was to decrease post-operative infections through development of a surgical antibiotic prophylaxis policy based on institution-specific risk factors and microbiology data.

Methods

We conducted a retrospective review of deep wound infections at our institution over a 5-year period (2014–2018). 399 spinal fusion procedures were performed with a 2.5% post-operative infection rate. Patients with neuromuscular scoliosis were six times more likely to develop deep wound infections (7.6%) compared to patients with congenital and idiopathic scoliosis (combined rate of 1.25%). The microbiology data revealed that polymicrobial, extended spectrum beta-lactamase (ESBL) gram negative organisms predominated in patients with neuromuscular scoliosis. Based on these findings, we implemented an evidence-based quality improvement intervention: all patients with neuromuscular scoliosis undergoing spinal fusion were given a single 15 mg/kg dose of amikacin, in addition to our standard practice of perioperative cefazolin plus vancomycin with intra-operative betadine wash and vancomycin powder application. This intervention was put into practice in January 2019.

Results

Since the implementation of our quality improvement initiative, the overall post-operative infection rate decreased to 1.1% (2 infections in 176 cases). Ninety-eight percent of the 43 neuromuscular scoliosis patients who underwent spinal fusion in the post-intervention time frame have remained infection free.

Conclusion

Examination of post-operative infection and microbiology data at the institution level can guide the development of institution specific, evidence-based quality improvement initiatives that reduce post-operative wound infections.
Methodology and political science: the discipline needs three fundamentally different methodological traditions
Authors: Lauer Johann
Published: January 2021

Science is driven by methodology. In this article, I will show that political science, like all other social sciences, can draw on three fundamentally different methodological traditions. The first is adescriptive traditionto generate descriptive knowledge to describe political phenomena and interpret political symbols (text, image, audio and video). The second is anexplanatory-prognostic traditionto generate explanatory and prognostic knowledge to explain and predict political events. The third is agenuine practical(not applied!)traditionto generate and scientifically legitimate practical knowledge for political standardization and regulation. Furthermore, I will show that political science would greatly increase its relevance to practical politics and to society if all methodological traditions within the discipline were usedcomplementarilyand applied in their updated forms. The descriptive tradition makes it possible to describe political reality as well as sociologists, for example, do. The explanatory-prognostic tradition is necessary to be heard alongside the economic sciences. The practical tradition allows catching up with jurisprudence.
School‐related pressure and parental support as predictors of change in student stress levels from early to middle adolescence
Authors: Kulakow Stefan , Raufelder Diana , Hoferichter Frances
Published: January 2021

Introduction

One major stressor of adolescents relates to the pressure students perceive from their parents accompanied by high academic expectations, while in contrast parental support is related to low levels of stress. However, it is not clear whether the perceived parental pressure and support contribute to a change in students’ stress level from early to middle adolescence and if there are differences among students from low vs. high track schools.

Methods

Thus, based on the conservation of resources theory, this two‐wave study examined the role of perceived maternal and paternal pressure and support for students’ general stress level from grades 8 to 9 among students attending high‐ and low‐track schools by applying multigroup multilevel latent change modeling based on data from 1088 8th grade students (M Age = 13.70, SD = 0.53, 53.9% girls at Time 1).

Results

Results indicate that the general stress level from early to middle adolescence increases for students from low‐track schools only. This change increases even more if students from lowtrack schools perceive pressure from their fathers, whereas perceived paternal support dampen the increase of stress. For students from high‐track schools, perceived maternal pressure is positively and maternal support negatively related to students’ stress level in grade 8. Overall, boys tend to report lower stress levels compared to girls.

Conclusions

The findings of the present study support the conservation of resources theory, as both perceived support and pressure from parents and the educational context predict students’ stress development during adolescence.
The right side of the tracks
Authors: Nickels Liz
Published: January 2021

Liz Nickels spoke to rail giant Wabtec’s global additive manufacturing (AM) leader to discuss the company’s increasing focus on metal 3D printed parts for both freight and transit.
Automotive composites post-Covid – highs and lows
Authors: Nickels Liz
Published: January 2021

How might Covid affect the use of composites in auto applications? Reinforced Plastics spoke to Eric Haiss, global director of automotive business development at global thermoset specialist IDI Composites about the history of the company and the way forward.
The association of milk and dairy consumption with iodine status in pregnant women in Oporto region
Authors: Ferreira Pedro , Pinheiro Cátia , Matta Coelho Cláudia , Guimarães Juliana , Pereira Gonçalo , Xavier Moreira Nara , Cortez Alice , Bracchi Isabella , Pestana Diogo , Barreiros Mota Inês , Prucha Carmo , Martins Cristina , Alves Ribeiro Célia , Pinto Edgar , Almeida Agostinho , Delerue-Matos Cristina , Montenegro Nuno , Dias Cláudia Camila , Moreira-Rosário André , Azevedo Luís Filipe , Brantsæter Anne-Lise , Ramalho Carla , Cruz Fernandes Virgínia , Calhau Conceição , Costa Leite João , Keating Elisa
Published: January 2021

The role of milk and dairy products in supplying iodine to pregnant women is unknown in Portugal. The aim of this study was to evaluate the association between milk and dairy product consumption and the iodine status of pregnant women in the IoMum cohort of the Oporto region. Pregnant women were recruited between 10 and 13 weeks of gestation, when they provided a spot urine sample and information on lifestyle and intake of iodine-rich foods. Urinary iodine concentration (UIC) was determined by inductively coupled plasma MS. A total of 468 pregnant women (269 iodine supplement users and 199 non-supplement users) were considered eligible for analysis. Milk (but not yogurt or cheese) intake was positively associated with UIC, in the whole population (P = 0·02) and in the non-supplement users (P = 0·002), but not in the supplement users (P = 0·29). In non-supplement users, adjusted multinomial logistic regression analysis showed that milk consumption <3 times/month was associated with a five times increased risk of having UIC < 50 µg/l when compared with milk consumption ≥2 times/d (OR 5·4; 95 % CI 1·55, 18·78; P = 0·008). The highest UIC was observed in supplement users who reported consuming milk once per d (160 µg/l). Milk, but not yogurt or cheese, was positively associated with iodine status of pregnant women. Despite the observed positive association, daily milk consumption may not be sufficient to ensure adequate iodine intake in this population.
Description of Hemicycliophora cardamomi sp. n. (Nematoda: Hemicycliophoridae) associated with Amomum longiligulare T.L. Wu and a web-based key for the identification of Hemicycliophora spp.
Authors: Nguyen H.T. , Trinh Q.P. , Couvreur M. , Nguyen T.D. , Bert W.
Published: January 2021

During a survey of plant diseases in the Central Highlands of Vietnam, a new plant-parasitic nematode, Hemicycliophora cardamomi sp. n., was discovered in the growing areas of Amomum longiligulare, a valuable medicinal plant with high economical value. The new species is characterized by a cuticular sheath loosely fitting body; a labial region continuous to the body contour bearing 3 annuli; a lateral field frequently marked by a discontinuous single line with indistinct additional short lines in some parts along the body; a modified vulval lip with an anterior lip c. 4 annuli long; and a post-vulval body tapering to a pointed tail tip with an abrupt constriction at the last third of the post-vulval body. Morphology and molecular characterizations of D2-D3 expansion segment of 28S rRNA, ITS, and COI mtDNA gene regions clearly distinguish the new species from all other 133 known species. This study also provides a newly developed web-based key for the identification of Hemicycliophora spp. in order to accelerate the identification process, to minimize the increasing error load associated with larger datasets, and to avoid any dependence on a single starting entry. This key includes both an existing Bray–Curtis similarity measure and a newly developed similarity formula.
Mobilizing Bodies and Body Parts, from Myanmar to Manipur: Medical connections through borderlands in ‘transition’
Authors: MCDUIE-RA DUNCAN
Published: January 2021

This article focuses on cross-border medical connections between Myanmar and Manipur, India. Non-state actors have been instrumental in creating the networks to bring bodies and body parts back and forth, first bypassing, then enmeshing, state actors. I focus on the movement of patients and medical samples across the border—from western Myanmar to Imphal city and back again—and the health infrastructure that enables it. Analysing these connections makes several contributions to the study of border governance. First, movement from Myanmar to Manipur is primarily for treatment or diagnosis, and these connections project particular ways of thinking about each place—western Myanmar as poor and remote, Manipur as advanced and networked. Second, both Manipur and western Myanmar can be considered in ‘transition’—as territories being recalibrated by political dynamics emanating elsewhere yet becoming connected through shared needs. Third, patients and samples move through territories controlled by paramilitary forces, underground groups, and different tribal councils. Routes are sometimes blocked or passage treacherous, testing the limits of conventional notions of bilateral border governance. Finally, cross-border medical connections between Manipur and Myanmar draw attention to the risky cross-border medical mobility of the poor. Rather than seeking to minimize cost, patients utilize Manipur's health infrastructure out of necessity, providing insights into the contours of cross-border medical care in times of transition.
Boundary Making in Myanmar's Electoral Process: Where elections do not take place
Authors: LIDAUER MICHAEL
Published: January 2021

The 2015 general elections were considered a hallmark of Myanmar's transition from an authoritarian regime towards a new form of government. However, the elections did not take place in all parts of the country, and significant portions of the population were excluded from the vote, including voters in areas of contested sovereignty, those who experienced displacement by conflict, and the Rohingya. Against the background of the regulatory framework for elections in Myanmar and its electoral system, this article looks first into a particular understudied element of the electoral process—the cancellation or postponement of elections in areas affected by conflict, which is little understood by voters, election administrators, and outside observers. Second, the article examines the conditions necessary for internally displaced persons (IDPs) to participate in elections. Third, the article recapitulates the gradual legal disenfranchisement of voters and candidates who self-identified as Rohingya, which preceded their mass exodus to Bangladesh in 2017. While in 2015 all these processes of exclusion were arguably of lesser priority for the election administration, which facilitated the first credible Myanmar election in decades, they have not altered significantly since and will affect the electoral participation of disadvantaged communities again in the future.
Fully Distributed Region-Reaching Control with Collision Avoidance for Multi-robot Systems
Authors: Yu Jinwei , Ji Jinchen , Miao Zhonghua , Zhou Jin
Published: January 2021

This paper proposes a fully distributed continuous region-reaching controller for multi-robot systems which can effectively eliminate the chattering issues and the negative effects caused by discontinuities. The adaptive control gain technique is employed to solve the distributed region-reaching control problem. By performing Lyapunov function-based stability analysis, it is shown that all the robots can move cohesively within the desired region under the proposed distributed control algorithm. In addition, collision avoidance and velocity matching within the moving region can be guaranteed under properly designed control gains. Simulation examples are given to verify the capabilities of the proposed control method.
Research Career Paths Among Political Scientists in Research Institutions
Authors: Hill Kim Quaile
Published: January 2021

Considerable research assesses research success in political science. Yet this work has not considered widespread findings that scholars can follow various career research paths that complicate how we envision scholarly success. Further, we have no systematic information on these career paths in any scientific discipline. I present an empirically validated research career-path typology for political scientists who began their career in a research institution. The typology demonstrates that many scholars follow paths different from the most conventional expectations, and research success by measures of publications and citations is associated with only some of these paths. Thus, existing research on aggregate publications and citations likely addresses only a subset of the career paths revealed in this article. Understanding research activities in our profession requires consideration of the various career paths, their motivation, and their place in our research community.
Performance on the ROCF at 8 Years Predicts Academic Achievement at 16 Years in Individuals with Dextro-Transposition of the Great Arteries
Authors: Fasano-McCarron Matthew E. , Bernstein Jane Holmes , Waber Deborah P. , Newburger Jane W. , DeMaso David R. , Bellinger David C. , Cassidy Adam R.
Published: January 2021

Objective:

This study examined longitudinal associations between performance on the Rey–Osterrieth Complex Figure–Developmental Scoring System (ROCF-DSS) at 8 years of age and academic outcomes at 16 years of age in 133 children with dextro-transposition of the great arteries (d-TGA).

Method:

The ROCF-DSS was administered at the age of 8 and the Wechsler Individual Achievement Test, First and Second Edition (WIAT/WIAT-II) at the ages of 8 and 16, respectively. ROCF-DSS protocols were classified by Organization (Organized/Disorganized) and Style (Part-oriented/Holistic). Two-way univariate (ROCF-DSS Organization × Style) ANCOVAs were computed with 16-year academic outcomes as the dependent variables and socioeconomic status (SES) as the covariate.

Results:

The Organization × Style interaction was not statistically significant. However, ROCF-DSS Organization at 8 years was significantly associated with Reading, Math, Associative, and Assembled academic skills at 16 years, with better organization predicting better academic performance.

Conclusions:

Performance on the ROCF-DSS, a complex visual-spatial problem-solving task, in children with d-TGA can forecast academic performance in both reading and mathematics nearly a decade later. These findings may have implications for identifying risk in children with other medical and neurodevelopmental disorders affecting brain development.
Constitutional Adjudication and Constitutional Politics in the United Kingdom: The Miller II Case in Legal and Political Context
Authors: Elliott Mark
Published: January 2021

For a number of years there has been nothing at all unusual about the United Kingdom finding itself in a state of constitutional upheaval; indeed, for some time, this has been the UK constitution’s default setting. This has sometimes been as a result of long-anticipated and carefully planned reforms, such as the enactment, in the late 1990s, of legislation to give domestic effect to the European Convention on Human Rights and to introduce devolved systems of government in Scotland, Wales and Northern Ireland. In contrast, more recent upheaval is attributable to often unexpected reactions to often unexpected events. For example, legislation making substantial changes to the devolution scheme in Scotland – providing, among other things, for the constitutional permanence of the Scottish Parliament and Government – was enacted to implement panicked promises made by UK politicians in the dying days of the Scottish independence referendum campaign, at which point a vote in favour of independence seemed a distinct possibility. And then, needless to say, there is Brexit – about which it is almost impossible to be guilty of hyperbole when describing its constitutional implications, so numerous and potentially far-reaching are they.
A Pilot Study on the Effect of an Energy Drink on Interoception in	High vs. Low Anxiety Sensitivity Individuals
Authors: Mai-Lippold Sandra A. , Dettlinger Clara M. , Khalsa Sahib S. , Pollatos Olga
Published: January 2021

Abstract. Background: The market for energy drinks has grown quickly over the past 20 years. While the physiological and psychological effects of different ingredients have been studied, the influence of energy drinks on interoceptive processes is unclear. Anxiety has been associated with amplified interoceptive functioning, suggesting potentially exaggerated reactions to energy drinks. Aims: Investigate the effect of energy drink consumption and anxiety sensitivity (AS) as well as their possible interactions on cardiorespiratory dimensions of interoception. Method: Thirty-nine healthy students consumed an energy drink via a placebo-controlled, counterbalanced, crossover design. Cardiac and respiratory interoceptive accuracy (IAcc), interoceptive sensibility (IS), and interoceptive evaluation (IE) were assessed. Heartbeat-evoked potentials (HEPs) were analyzed to evaluate neural processing of the heartbeat. Results: Consumption of one energy drink did not influence IAcc, IS, or IE. However, high AS subjects reported reduced interoceptive confidence after energy drink intake. While HEP amplitudes did not differ depending on the type of drink, high AS subjects showed reduced HEPs overall compared to low AS subjects. Heart rate was significantly lower following energy drink consumption as compared to the placebo condition. Limitations: The sample size was small, energy dosages low, and physiological parameters should be assessed in more detail. Conclusion: Energy drink consumption was associated with an interoceptive bias in high AS individuals suggesting possible interaction effects between changes in physical state, interoception, and anxiety.
Slow light bimodal interferometry in one-dimensional photonic crystal waveguides
Authors: Torrijos-Morán Luis , Griol Amadeu , García-Rupérez Jaime
Published: January 2021

Strongly influenced by the advances in the semiconductor industry, the miniaturization and integration of optical circuits into smaller devices has stimulated considerable research efforts in recent decades. Among other structures, integrated interferometers play a prominent role in the development of photonic devices for on-chip applications ranging from optical communication networks to point-of-care analysis instruments. However, it has been a long-standing challenge to design extremely short interferometer schemes, as long interaction lengths are typically required for a complete modulation transition. Several approaches, including novel materials or sophisticated configurations, have been proposed to overcome some of these size limitations but at the expense of increasing fabrication complexity and cost. Here, we demonstrate for the first time slow light bimodal interferometric behaviour in an integrated single-channel one-dimensional photonic crystal. The proposed structure supports two electromagnetic modes of the same polarization that exhibit a large group velocity difference. Specifically, an over 20-fold reduction in the higher-order-mode group velocity is experimentally shown on a straightforward all-dielectric bimodal structure, leading to a remarkable optical path reduction compared to other conventional interferometers. Moreover, we experimentally demonstrate the significant performance improvement provided by the proposed bimodal photonic crystal interferometer in the creation of an ultra-compact optical modulator and a highly sensitive photonic sensor.
ARNT deficiency represses pyruvate dehydrogenase kinase 1 to trigger ROS production and melanoma metastasis
Authors: Huang Chi-Ruei , Chang Ting-Wei , Lee Chung-Ta , Shen Chih-Jie , Chang Wen-Chang , Chen Ben-Kuen
Published: January 2021

The metabolic changes in melanoma cells that are required for tumor metastasis have not been fully elucidated. In this study, we show that the increase in glucose uptake and mitochondrial oxidative phosphorylation confers metastatic ability as a result of aryl hydrocarbon receptor nuclear translocator (ARNT) deficiency. In clinical tissue specimens, increased ARNT, pyruvate dehydrogenase kinase 1 (PDK1), and NAD(P)H quinine oxidoreductase-1 (NQO1) was observed in benign nevi, whereas lower expression was observed in melanoma. The depletion of ARNT dramatically repressed PDK1 and NQO1 expression, which resulted in an increase of ROS levels. The elimination of ROS using N-acetylcysteine (NAC) and inhibition of oxidative phosphorylation using carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and rotenone inhibited the ARNT and PDK1 deficiency-induced cell migration and invasion. In addition, ARNT deficiency in tumor cells manipulated the glycolytic pathway through enhancement of the glucose uptake rate, which reduced glucose dependence. Intriguingly, CCCP and NAC dramatically inhibited ARNT and PDK1 deficiency-induced tumor cell extravasation in mouse models. Our work demonstrates that downregulation of ARNT and PDK1 expression serves as a prognosticator, which confers metastatic potential as the metastasizing cells depend on metabolic changes.
Design, performance, and demand for a novel in-line chlorine doser to increase safe water access
Authors: Powers Julie E. , McMurry Cynthia , Gannon Sarah , Drolet Adam , Oremo Jared , Klein Linden , Crider Yoshika , Davis Jennifer , Pickering Amy J.
Published: January 2021

Financially sustainable strategies are needed to increase access to safe drinking water in low-income settings. We designed a novel in-line chlorine doser that employs the Venturi principle to automatically add liquid chlorine at the point of water collection (tap outflows). The Venturi does not require electricity or moving parts, and users do not have to change the way they typically collect water. We field-tested the Venturi and assessed its technical performance and sales viability at water kiosks in Kisumu County, Kenya. We offered kiosk owners 6-month service packages to lease or lease-to-own the device; 27% of kiosks given a sales pitch committed to a service package. All but one kiosk paid in full during the 6-month service period and more than two-thirds purchased the device with payments totaling >$250 USD per kiosk. Kiosk customers could choose to purchase chlorinated or unchlorinated water from separate taps; 66% reported buying chlorinated water. Kiosk taps fitted with the Venturi had detectable free chlorine residual 97.6% of the time. The technical performance of the Venturi and effective demand from kiosks indicate high potential for the Venturi to increase safe water access in low-income communities.
Dataset of spiking and LFP activity invasively recorded in the human amygdala during aversive dynamic stimuli
Authors: Fedele Tommaso , Boran Ece , Chirkov Valerii , Hilfiker Peter , Grunwald Thomas , Stieglitz Lennart , Jokeit Hennric , Sarnthein Johannes
Published: January 2021

We present an electrophysiological dataset collected from the amygdalae of nine participants attending a visual dynamic stimulation of emotional aversive content. The participants were patients affected by epilepsy who underwent preoperative invasive monitoring in the mesial temporal lobe. Participants were presented with dynamic visual sequences of fearful faces (aversive condition), interleaved with sequences of neutral landscapes (neutral condition). The dataset contains the simultaneous recording of intracranial EEG (iEEG) and neuronal spike times and waveforms, and localization information for iEEG electrodes. Participant characteristics and trial information are provided. We technically validated this dataset and provide here the spike sorting quality metrics and the spectra of iEEG signals. This dataset allows the investigation of amygdalar response to dynamic aversive stimuli at multiple spatial scales, from the macroscopic EEG to the neuronal firing in the human brain.
Morphological and genetic characteristics of the novel entomopathogenic fungus Ophiocordyceps langbianensis (Ophiocordycipitaceae, Hypocreales) from Lang Biang Biosphere Reserve, Vietnam
Authors: Lao Thuan Duc , Le Thuy Ai Huyen , Truong Nguyen Binh
Published: January 2021

An entomopathogenic fungus newly named Ophiocordyceps langbianensis was collected from Lang Biang Biosphere Reserve, located in Lam Dong Province, Vietnam. It is characterized as a species of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) having the unique characteristics of a cylindrical fertile part and several branched apical appendices. Each ascospore develops as two swollen, constricted part-spores. A phylogenetic analysis of multiple genes, including nrLSU, nrSSU, Rpb1, ITS and Tef, supported its systematic position in the genus of Ophiocordyceps; it is related to O. brunneipunctata. Based on morphological and phylogenetic analyses, O. langbianensis was confirmed as a new species from Vietnam.
The demethylase inhibitor GSK-J4 limits inflammatory colitis by promoting de novo synthesis of retinoic acid in dendritic cells
Authors: Doñas Cristian , Neira Jocelyn , Osorio-Barrios Francisco , Carrasco Macarena , Fernández Dominique , Prado Carolina , Loyola Alejandra , Pacheco Rodrigo , Rosemblatt Mario
Published: January 2021

Dendritic cells (DCs) promote T-cell mediated tolerance to self-antigens and induce inflammation to innocuous-antigens. This dual potential makes DCs fundamental players in inflammatory disorders. Evidence from inflammatory colitis mouse models and inflammatory bowel diseases (IBD) patients indicated that gut inflammation in IBD is driven mainly by T-helper-1 (Th1) and Th17 cells, suggesting an essential role for DCs in the development of IBD. Here we show that GSK-J4, a selective inhibitor of the histone demethylase JMJD3/UTX, attenuated inflammatory colitis by reducing the inflammatory potential and increasing the tolerogenic features of DCs. Mechanistic analyses revealed that GSK-J4 increased activating epigenetic signals while reducing repressive marks in the promoter of retinaldehyde dehydrogenase isoforms 1 and 3 in DCs, enhancing the production of retinoic acid. This, in turn, has an impact on regulatory T cells (Treg) increasing their lineage stability and gut tropism as well as potentiating their suppressive activity. Our results open new avenues for the treatment of IBD patients.
Total cholesterol, arterial stiffness, and systolic blood pressure: a mediation analysis
Authors: Chen Haojia , Chen Youren , Wu Weiqiang , Cai Zefeng , Chen Zhichao , Yan Xiuzhu , Wu Shouling
Published: January 2021

On the basis of 45,092 participants (mean age of 54.04 ± 13.09 years) from the Kailuan study, this study was performed to explore the relationships among total cholesterol (TC), brachial-ankle pulse wave velocity (BaPWV), and systolic blood pressure (SBP) and quantify their separate effects. The correlations among TC, SBP, and BaPWV were analyzed using multivariate linear regression models. Mediation analysis was performed to determine whether the effect of TC on SBP can be explained by arterial stiffness. Multivariate linear regression analysis showed that for every one standard deviation increase in TC and BaPWV, SBP increased by 0.33 mmHg and 0.044 mmHg, respectively; for every one standard deviation increase in TC, BaPWV increased by 5.34 cm/s. Mediation analysis showed that the TC-induced SBP elevation was mediated by arterial stiffness in more than half of the whole cohort (indirect effect, 0.73; percent mediated, 54.5%). Furthermore, the TC-induced SBP elevation was mediated by arterial stiffness in less than half of the males (indirect effect, 0.70; percent mediated, 47.9%); however, the results were not statistically significant in females. In conclusion, TC and BaPWV are positively correlated with SBP, whereas TC is positively correlated with BaPWV. Almost half of the increase in SBP contributed to TC is mediated by arterial stiffness.
Reduced vagal tone in women with endometriosis and auricular vagus nerve stimulation as a potential therapeutic approach
Authors: Hao Meihua , Liu Xishi , Rong Peijing , Li Shaoyuan , Guo Sun-Wei
Published: January 2021

Sensory and sympathetic nerves have been shown to promote the progression of endometriosis through the release of neuromediators and the lesional activation of respective receptors. The role of vagus nerves (VN) in lesional progression, however, is completely unclear, despite the signs suggestive of increased sympathetic tone in women with endometriosis. This study was undertaken to investigate whether VN plays any role in the progression of endometriosis. We recruited 45 patients with endometriosis and 42 healthy women, who were given electrocardiogram test and their heart rate variability was evaluated. In addition, three prospective, and randomized mouse experiments were conducted that evaluated, respectively, the effect of vagotomy, the effect of VN stimulation (VNS), and the therapeutic potential of VNS after the endometriosis was well established. All lesions were excised, weighed, and processed for immunohistochemistry and histochemistry analysis of select markers for lesional progression and fibrosis. We found that endometriosis patients exhibited reduced vagal activity as compared with controls, indicative of disrupted autonomic balance. Vagotomy increased while VNS decreased the lesion weight as compared with control mice, concomitant with more progressive and retarded lesion development and fibrogenesis, respectively. In addition, VNS demonstrated promising therapeutic effect, as evidenced by significantly reduced lesion weight, more attenuated lesional progression concomitant with improved hyperalgesia. Taken together, our data indicate that VN activity may play a dampening role in the progression of endometriosis. Consequently, boosting the VN activity may have therapeutic potentials for patients with endometriosis.
OpenEP: an open-source simulator for electroporation-based tumor treatments
Authors: Marino Matías , Luján Emmanuel , Mocskos Esteban , Marshall Guillermo
Published: January 2021

Electroporation (EP), the increase of cell membrane permeability due to the application of electric pulses, is a universal phenomenon with a broad range of applications. In medicine, some of the foremost -based tumor treatments are electrochemotherapy (ECT), irreversible electroporation, and gene electrotransfer (GET). The electroporation phenomenon is explained as the formation of cell membrane pores when a transmembrane cell voltage reaches a threshold value. Predicting the outcome of an -based tumor treatment consists of finding the electric field distribution with an electric threshold value covering the tumor (electroporated tissue). Threshold and electroporated tissue are also a function of the number of pulses, constituting a complex phenomenon requiring mathematical modeling. We present , an open-source specific purpose simulator for -based tumor treatments, modeling among other variables, threshold, and electroporated tissue variations in time. Distributed under a free/libre user license, allows the customization of tissue type; electrode geometry and material; pulse type, intensity, length, and frequency. facilitates the prediction of an optimal -based protocol, such as ECT or GET, defined as the critical pulse dosage yielding maximum electroporated tissue with minimal damage. displays a highly efficient shared memory implementation by taking advantage of parallel resources; this permits a rapid prediction of optimal -based treatment efficiency by pulse number tuning.
Conformational plasticity underlies membrane fusion induced by an HIV sequence juxtaposed to the lipid envelope
Authors: de la Arada Igor , Torralba Johana , Tascón Igor , Colom Adai , Ubarretxena-Belandia Iban , Arrondo José L. R. , Apellániz Beatriz , Nieva José L.
Published: January 2021

Envelope glycoproteins from genetically-divergent virus families comprise fusion peptides (FPs) that have been posited to insert and perturb the membranes of target cells upon activation of the virus-cell fusion reaction. Conserved sequences rich in aromatic residues juxtaposed to the external leaflet of the virion-wrapping membranes are also frequently found in viral fusion glycoproteins. These membrane-proximal external regions (MPERs) have been implicated in the promotion of the viral membrane restructuring event required for fusion to proceed, hence, proposed to comprise supplementary FPs. However, it remains unknown whether the structure–function relationships governing canonical FPs also operate in the mirroring MPER sequences. Here, we combine infrared spectroscopy-based approaches with cryo-electron microscopy to analyze the alternating conformations adopted, and perturbations generated in membranes by CpreTM, a peptide derived from the MPER of the HIV-1 Env glycoprotein. Altogether, our structural and morphological data support a cholesterol-dependent conformational plasticity for this HIV-1 sequence, which could assist cell-virus fusion by destabilizing the viral membrane at the initial stages of the process.
Reduced renal function may explain the higher prevalence of hyperuricemia in older people
Authors: Wang Yutang , Zhang Wanlin , Qian Tingting , Sun Hui , Xu Qun , Hou Xujuan , Hu Wenqi , Zhang Guang , Drummond Grant R. , Sobey Christopher G. , Charchar Fadi J. , Golledge Jonathan , Yang Guang
Published: January 2021

This study aimed to investigate the contribution of renal dysfunction to enhanced hyperuricemia prevalence in older people. A cohort of 13,288 Chinese people aged between 40 and 95 years were recruited from January to May 2019. Serum uric acid concentration and estimated glomerular filtration rate [eGFR] were measured. The associations between age or eGFR and serum uric acid or hyperuricemia were analyzed using linear or binary logistic regression adjusting for risk factors. Uric acid concentration and prevalence of hyperuricemia were greater in older participants. Adjustment for reduced renal function (eGFR < 60 mL/min/1.73 m2) eliminated the associations between older age and higher uric acid concentration and between older age and higher prevalence of hyperuricemia diagnosis, whereas adjustment for other risk factors did not change those associations. Lower eGFR was associated with higher uric acid concentration both before (β = − 0.296, P < 0.001) and after adjustment for age (β = − 0.313, P < 0.001). Reduced renal function was associated with hyperuricemia diagnosis both before (odds ratio, OR, 3.64; 95% CI 3.10–4.28; P < 0.001) and after adjustment for age (adjusted OR, 3.82; 95% CI 3.22–4.54; P < 0.001). Mean serum uric acid and prevalence of hyperuricemia were higher in people with eGFR < 60 mL/min/1.73 m2 than those with eGFR ≥ 60 mL/min/1.73 m2. The prevalence of reduced renal function increased with older age (P < 0.001). This study suggests that reduced renal function can explain the increased uric acid levels and hyperuricemia diagnoses in older people.
Usnic acid deteriorates acidogenicity, acidurance and glucose metabolism of Streptococcus mutans through downregulation of two-component signal transduction systems
Authors: Priya Arumugam , Kumar Chandra Bose Manish , Valliammai Alaguvel , Selvaraj Anthonymuthu , Pandian Shunmugiah Karutha
Published: January 2021

The principal etiological agent of human dental caries, Streptococcus mutans is a multi-virulent pathogen that can transform commensal oral microbial community to plaque biofilms. Major virulence factors that are associated with the cariogenicity of S. mutans include adhesion, acidogenicity and acidurity. All these pathogenic traits coordinate and alter the dental plaque ecology which provide room for interaction with other similar acidogenic and aciduric bacteria. This cariogenic flora increases the possibility of enamel demineralization which headway to caries development. The present study was aimed at evaluating the antimicrobial and antiinfective potential of a lichen secondary metabolite usnic acid (UA) against S. mutans. Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC) and growth kinetics were evaluated to determine the antimicrobial potential of UA against S. mutans. UA at 5 µg mL−1 and 10 µg mL−1 concentration were considered as MIC and MBC respectively. Effect on biofilm formation was microscopically assessed and found to be reduced in a concentration dependent manner. Gene expression of gtfB, gtfC, gtfD, vicR, ComDE and smu0630 was found to be downregulated upon treatment with sub-MIC of UA. Acidogenicity, acidurity, eDNA synthesis and response to oxidative stress were found to be attenuated by the influence of UA. It was also demonstrated to act on preformed mature biofilm of S. mutans. Moreover, UA was shown to possess very low frequency to acquire spontaneous resistance development in S. mutans. Besides, no morphological aberrations or toxic effect was instigated by UA in the human buccal epithelial cells as well as to the oral commensals. Altogether, these results demonstrate the therapeutic potential of usnic acid in the treatment of S. mutans infection.
Drivers of spatio-temporal variation in mosquito submissions to the citizen science project ‘Mückenatlas’
Authors: Pernat Nadja , Kampen Helge , Ruland Florian , Jeschke Jonathan M. , Werner Doreen
Published: January 2021

Intensified travel activities of humans and the ever growing global trade create opportunities of arthropod-borne disease agents and their vectors, such as mosquitoes, to establish in new regions. To update the knowledge of mosquito occurrence and distribution, a national mosquito monitoring programme was initiated in Germany in 2011, which has been complemented by a citizen science project, the ‘Mückenatlas’ since 2012. We analysed the ‘Mückenatlas’ dataset to (1) investigate causes of variation in submission numbers from the start of the project until 2017 and to (2) reveal biases induced by opportunistic data collection. Our results show that the temporal variation of submissions over the years is driven by fluctuating topicality of mosquito-borne diseases in the media and large-scale climate conditions. Hurdle models suggest a positive association of submission numbers with human population, catch location in the former political East Germany and the presence of water bodies, whereas precipitation and wind speed are negative predictors. We conclude that most anthropogenic and environmental effects on submission patterns are associated with the participants’ (recording) behaviour. Understanding how the citizen scientists’ behaviour shape opportunistic datasets help to take full advantage of the available information.
Piglets can secrete acidic mammalian chitinase from the pre weaning stage
Authors: Kawasaki Kiyonori , Osafune Tomomi , Tamehira Saya , Yano Kiminobu
Published: January 2021

Fishmeal substitutes (such as insect-based feeds) in pig diets can promote sustainable pork production. Insect powders contain chitin, a nitrogen-containing indigestible material, and pigs must have the capacity to secrete chitin-degrading enzymes to benefit from these diets. The chitin-degrading enzyme (acidic mammalian chitinase; AMCase) and its gene expression have been detected in the stomach tissue of approximately 6-month-old fattening pigs; however, it remains unclear from which stage chitin-degrading enzymes are secreted. In the present study, the stomach tissue of piglets was collected from the suckling stage (14 d old) to 56 d to evaluate chitin-degrading enzymes and associated gene expression. AMCase mRNA and protein expression was detected in the stomach tissue of all piglets from days 14 to 56. AMCase secretion might increase with the increase in stomach tissue weight as piglets grow. Insect powders can therefore be used in the diets of pre-weaning piglets. The gastric AMCase level was approximately 30% that of fattening pigs. The appropriate inclusion of insect meals in the diets of pigs at different growth stages still needs to be determined.
Heterogeneously integrated ITO plasmonic Mach–Zehnder interferometric modulator on SOI
Authors: Amin Rubab , Maiti Rishi , Gui Yaliang , Suer Can , Miscuglio Mario , Heidari Elham , Khurgin Jacob B. , Chen Ray T. , Dalir Hamed , Sorger Volker J.
Published: January 2021

Densely integrated active photonics is key for next generation on-chip networks for addressing both footprint and energy budget concerns. However, the weak light-matter interaction in traditional active Silicon optoelectronics mandates rather sizable device lengths. The ideal active material choice should avail high index modulation while being easily integrated into Silicon photonics platforms. Indium tin oxide (ITO) offers such functionalities and has shown promising modulation capacity recently. Interestingly, the nanometer-thin unity-strong index modulation of ITO synergistically combines the high group-index in hybrid plasmonic with nanoscale optical modes. Following this design paradigm, here, we demonstrate a spectrally broadband, GHz-fast Mach–Zehnder interferometric modulator, exhibiting a high efficiency signified by a miniscule VπL of 95 V μm, deploying a one-micrometer compact electrostatically tunable plasmonic phase-shifter, based on heterogeneously integrated ITO thin films into silicon photonics. Furthermore we show, that this device paradigm enables spectrally broadband operation across the entire telecommunication near infrared C-band. Such sub-wavelength short efficient and fast modulators monolithically integrated into Silicon platform open up new possibilities for high-density photonic circuitry, which is critical for high interconnect density of photonic neural networks or applications in GHz-fast optical phased-arrays, for example.
Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf
Authors: Alba-Tercedor Javier , Hunter Wayne B. , Alba-Alejandre Ignacio
Published: January 2021

The Asian citrus psyllid (ACP),Diaphorina citri, is a harmful pest of citrus trees that transmitsCandidatusLiberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.
Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity
Authors: Neefjes M. , Housmans B. A. C. , van den Akker G. G. H. , van Rhijn L. W. , Welting T. J. M. , van der Kraan P. M.
Published: January 2021

Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood. Selection of the optimal reporter gene is crucial for study outcome. Here, we compared the characteristics of five reporter genes (Firefly luciferase, stable- and unstable Nano luciferase, secretable Gaussia luciferase and Red Fluorescent Protein) to study complex body fluids. For this comparison, the NFκB Response Element (NFκB-RE) and Smad Binding Element (SBE) were identically cloned into the five different reporter vectors. Reporter characteristics were evaluated by kinetic and concentration–response measurements in SW1353 and HeLa cell lines. Finally, reporter compatibility with complex body fluids (fetal calf serum, knee joint synovial fluid and human serum) and inter-donor variation were evaluated. Red Fluorescent Protein demonstrated poor inducibility as a reporter gene and slow kinetics compared to luciferases. Intracellularly measured luciferases, such as Firefly luciferase and Nano luciferase, revealed good compatibility with complex body fluids. Secreted Gaussia luciferase appeared to be incompatible with complex body fluids, due to variability in inter-donor signal interference. Unstable Nano luciferase demonstrated clear inducibility, high sensitivity and compatibility with complex body fluids and therefore can be recommended for cellular signaling studies using complex body fluids.
In-vacuum active colour sensor and wireless communication across a vacuum-air interface
Authors: Sakai Osamu , Kitagawa Takayuki , Sakurai Keiji , Itami Go , Miyagi Shigeyuki , Noborio Kazuyuki , Taguchi Kohshi
Published: January 2021

In situ sensing with wireless digital-data transfer is a potential processing scheme that works very closely to the location of an event monitored by a sensor and converts the sensor’s raw output into digitized and informative small-volume bits, as suggested by recent proposals for edge computing and the Internet of Things (IoT). Colour perception may be a target of in situ sensor data acquisition; however, in contrast to from other sensing devices, colour sensors that detect visible light signals are usually located away from light-emitting sources, collecting light transmitting through the space and attenuating it in some manner. For example, in a vacuum chamber whose gas pressure is much less than the ambient atmosphere in which the sensors usually work, there are many veiled light sources, such as discharge plasma, for various industrial purposes including nanoscale manufacturing. In this study, we designed an in-vacuum colour sensor that can work with analogue-to-digital conversion and transfer data by wireless communication; this sensor is active in a low-pressure plasma chamber, detecting light signals and transferring them to a personal computer located outside the vacuum chamber. In addition to detecting lights with controlled spectra from outside successfully, we achieved complete operation of our in-vacuum active sensor for plasma emissions generated at 100 Pa. Comparing the signals with data from simultaneous monitoring by a monochromator, we established that the recorded signals arose from the plasma, confirming successful direct detection of low-pressure plasma emissions without any filtering effects between the sensor and the target object.