bluuebunny/crossref_metadata_2025 huggingface dataset:
Micromorphology of Labellum in Selected Dendrobium Sw. (Orchidaceae, Dendrobieae)
Authors: Burzacka-Hinz Aleksandra , Narajczyk Magdalena , Dudek Magdalena , Szlachetko Dariusz L.
Published: August 2022

Dendrobium is one of the most species-rich genera of the Paleotropical orchids. It embraces more than 1000 species, most of which are epiphytes. The strong variation in floral characters causes many identification difficulties within this genus. One of the key structures, often sufficient in identification on a species level, is the labellum, which in many species of Dendrobium possesses a thickened callus and various types of trichomes and papillae. The aim of this study is to identify and describe the structures present on the labellum surface of the analyzed species, determine their distribution and density, as well as to check whether the obtained data have taxonomic value. In this paper, we present the results of a micromorphological study on the labellum of 21 species of Dendrobium, representing 13 sections, using scanning electron microscopy (SEM). Our studies revealed the presence of both uni- and multicellular structures on the surface of the labellum. We observed three types of trichomes (conical, cylindrical, ellipsoidal) and three types of papillae (conical, cylindrical, semicircular). Neither trichomes nor papillae were recorded for five species. In addition, we made diagrams showing the distribution and density of structures on the labellum. Based on the micromorphological results combined with the phylogenetic tree performed, we suggest that the presence/absence of labellum structures does not necessarily reflect the phylogenetic relationship and might be misleading, as in some cases, they arise due to convergence.
Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations
Authors: Cui Jie , Li Junliang , Dai Cuihong , Li Liping
Published: August 2022

Salinity is one of the most serious threats to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt; however, the genome-wide dynamic response to salt stress remains largely unknown in sugar beet. In the present study, physiological and transcriptome analyses of sugar beet leaves and roots were compared under salt stress at five time points. The results showed that different salt stresses influenced phenotypic characteristics, leaf relative water content and root activity in sugar beet. The contents of chlorophyll, malondialdehyde (MDA), the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were also affected by different salt stresses. Compared with control plants, there were 7391 and 8729 differentially expressed genes (DEGs) in leaves and roots under salt stress, respectively. A total of 41 hub genes related to salt stress were identified by weighted gene co-expression network analysis (WGCNA) from DEGs, and a transcriptional regulatory network based on these genes was constructed. The expression pattern of hub genes under salt stress was confirmed by qRT-PCR. In addition, the metabolite of sugar beet was compared under salt stress for 24 h. A total of 157 and 157 differentially accumulated metabolites (DAMs) were identified in leaves and roots, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further indicated that DEGs and DAMs act on the starch and sucrose metabolism, alpha-linolenic acid metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction pathway. In this study, RNA-seq, WGCNA analysis and untargeted metabolomics were combined to investigate the transcriptional and metabolic changes of sugar beet during salt stress. The results provided new insights into the molecular mechanism of sugar beet response to salt stress, and also provided candidate genes for sugar beet improvement.
Design, Synthesis, Molecular Modeling and Anti-Hyperglycemic Evaluation of Quinazoline-Sulfonylurea Hybrids as Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) and Sulfonylurea Receptor (SUR) Agonists
Authors: El-Zahabi Mohamed Ayman , Bamanie Faida H. , Ghareeb Salah , Alshaeri Heba K. , Alasmari Moudi M. , Moustafa Mohamed , Al-Marzooki Zohair , Zayed Mohamed F.
Published: August 2022

New quinazoline-sulfonylurea hybrids were prepared and examined for their in vivo anti-hyperglycemic activities in STZ-induced hyperglycemic rats using glibenclamide as a reference drug. Compounds VI-6-a, V, IV-4, VI-4-c, IV-6, VI-2-a, IV-1, and IV-2 were more potent than the reference glibenclamide. They induced significant reduction in the blood glucose levels of diabetic rats: 78.2, 73.9, 71.4, 67.3, 62, 60.7, 58.4, and 55.9%, respectively, while the reference glibenclamide had 55.4%. Compounds IV-1, VI-2-a, IV-2, V, and IV-6 showed more prolonged antidiabetic activity than glibenclamide. Moreover, molecular docking and pharmacokinetic studies were performed to examine binding modes of the prepared compounds against peroxisome proliferator-activated receptor gamma (PPARγ). The highest active compounds exhibited good binding affinity with high free energy of binding against PPARγ. In silico absorption, distribution, metabolism, elimination and toxicity (ADMET) studies were performed to investigate pharmacokinetics and safety of the synthesized compounds. They showed considerable human intestinal absorption with low toxicity profile.
Essential Oils of Tagetes minuta and Lavandula coronopifolia from Djibouti: Chemical Composition, Antibacterial Activity and Cytotoxic Activity against Various Human Cancer Cell Lines
Authors: Abdoul-Latif Fatouma Mohamed , Elmi Abdirahman , Merito Ali , Nour Moustapha , Risler Arnaud , Ainane Ayoub , Bignon Jérôme , Ainane Tarik
Published: August 2022

The chemical composition of the essential oils of two plants (Tagetes minuta L. and Lavandula coronopifolia L.) harvested from the Day region (in the north of Djibouti) is the subject of this study. The extraction of essential oils was carried out by hydrodistillation, and the average yield was obtained at a rate of approximately 0.25% for Tagetes minuta L. and 0.42% for Lavandula coronopifolia L. The analyses of these essential oils by gas chromatography coupled with mass spectrometry identified 13 compounds in the essential oil of Tagetes minuta L., including dihydrotagetone (20.8%), artemisia (17.9%), (Z)-tagetenone (12.4%), (-)-spathulenol (11.0%) and estragole (9.5%), were obtained as majority compounds, with a percentage of 71.6%. The essential oil of Lavandula coronopifolia L. is characterized by the presence of 42 compounds, including cis-caryophyllene (18.9%), dehydronerolidol (12.8%), isolongifolanone (11.2%), caryophyllene oxide (8.2%), 10-epi-β-eudesmol (7.7%) and humulene (5.1%), were obtained as the majority chemical constituents, with a percentage of 63.9%. The antimicrobial activities of the essential oils at concentrations of 5% were measured against 12 bacterial strains (Gram positive: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Streptococcus agalactiae (ATCC 27956), Staphylococcus epidermidis and Corynebacterium sp.; Gram Negative: Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606), Shigella sonnei (ATCC 9290), Salmonella enterica sv. Typhimurium (ATCC 13311) and Enterobacter cloacae), and the results of in vitro experiments showed inhibitory effects against most strains tested except Staphylococcus aureus, Enterococcus faecalis and Streptococcus agalactiae. Additionally, both oils were tested for their ability to selectively kill 13 human cancer cells (K562, A549, HCT116, PC3, U87-MG, MIA-Paca2, HEK293, NCI-N87, RT4, U2OS, A2780, MRC-5 and JIMT-T1), and the results obtained, according to the values of IC50, show the significant activity of two essential oils, particularly on the HCT116 and A2780 lines, which present values between 0.25 µg/mL and 0.45 µg/mL, respectively.
Analysis of MATSim Modeling of Road Infrastructure in Cyclists’ Choices in the Case of a Hilly Relief
Authors: Delhoum Younes , Belaroussi Rachid , Dupin Francis , Zargayouna Mahdi
Published: August 2022

For too long, many refined transportation models have focused solely on private and public transportation, assuming that bicycles only require simple models, such as bird flight distance or trips on horizontal tracks at a constant speed. This paper aims to study the impact of the road characteristics, such as road gradient, type of road and pavement surface of the road, on cyclists’ behavior using dedicated modules of MATSim. For that, we compare two approaches: a standard approach which does not consider the road characteristics, and a second approach that uses MATSim bicycle extension of Ziemke et al. The two approaches are analyzed over a sub-regional area around a district, focusing on a suburban city with an undulating relief made of average-to-steep hills. The focus is on the bicycle transportation model because the catchment area has a particularly challenging altitude profile and a large variety of roads, whether in type—from residential to national highway—or in pavement surface due to the number of green areas, such as parks and forests. This area is defined as a rather large 7 × 12 km, including five suburban cities in the South of Paris, France. A synthetic population of 126,000 agents was generated at a regional scale, with chains of activity made of work, education, shopping, leisure, restaurant and kindergarten, with activity-time choice, location choice and modal choice. We wanted to know how accurately a standard model of bicycle travels can be made with a 2D flat Earth assumption by comparing it to an algorithm extension that explicitly considers road characteristics in cyclists’ route choices. Our finding is that the MATSim bicycle extension model impacts mainly the long trips. Otherwise, the differences are minimal between the two models in terms of travel time and travel distance.
Carbon Fiber Implants in Orthopaedic Oncology
Authors: Yeung Caleb M. , Bhashyam Abhiram R. , Patel Shalin S. , Ortiz-Cruz Eduardo , Lozano-Calderón Santiago A.
Published: August 2022

Carbon fiber offers numerous material benefits including reduced wear, high strength-to-weight ratio, a similar elastic modulus to that of bone, and high biocompatibility. Carbon fiber implants are increasingly used in multiple arenas within orthopaedic surgery, including spine, trauma, arthroplasty, and oncology. In the orthopaedic oncologic population, the radiolucency of carbon fiber facilitates post-operative imaging for tumor surveillance or recurrence, the monitoring of bony healing and union, and radiation mapping and delivery.
Trends in Open vs. Endoscopic Carpal Tunnel Release: A Comprehensive Survey in Japan
Authors: Yamamoto Michiro , Curley James , Hirata Hitoshi
Published: August 2022

We analyzed trends in open and endoscopic carpal tunnel release (CTR) from 2014 to 2019 using the National Database of Health Insurance Claims and Specific Health Checkups in Japan (NDB). Japan has a universal health insurance system and more than 95% of all claims are searchable in the NDB open data repository. The results revealed that nearly 40,000 CTRs were performed annually in Japan, and open CTR was performed almost 4 times more often than endoscopic CTR. The crude annual incidence of CTR in the general population among people 20 years of age or older was 32.2 per 100,000. The incidence of open CTR peaked in the 80–84 age range for both males and females. The incidence of endoscopic CTR peaked at 80–84 years in females and at 75–79 years in males. There was a mild correlation coefficient between the endoscopic CTRs and the number of hand surgery specialists by prefecture per population (r = 0.32, p = 0.04). However, the number of hand surgeons per capita by region and open CTR per capita was not correlated (r = 0.06, p = 0.67). There were about twice as many outpatient as inpatient surgeries, reflecting a trend toward ambulatory treatment.
Superiority of MRI for Evaluation of Sacral Insufficiency Fracture
Authors: Yamauchi Taro , Sharma Sagar , Chandra Sarath , Tanaka Masato , Fujiwara Yoshihiro , Arataki Shinya , Sharma Ayush , Yokoyama Yusuke , Oomori Toshinori , Kanamaru Akihiro , Masuda Shin , Shimizu Noriyuki , Torigoe Kenta , Honda Osamu
Published: August 2022

Study Design: Retrospective observational study. Background: Sacral insufficiency fractures (SIF) are relatively rare fractures and difficult to diagnose on plain radiographs. The primary objective of the present study was to evaluate the role of lumbar magnetic resonance imaging (MRI) for the diagnosis of SIF. The secondary objective was to identify the classification of SIF by computed tomography (CT). Methods: A total of 77 (Male 11, female 66, mean 80.3 years) people were included in this study. Inclusion criteria for this study were: age ≥ 60 years and no history of high energy trauma. Exclusion criteria were high energy trauma and a current history of malignancy. Differences in the fracture detection and description in the various radiologic procedures were evaluated. Fracture patterns were evaluated with CT. The detection rates of additional pathologies in the MRI of the pelvis and lumbar spine were also recorded. Results: The sensitivities for SIF were 28.5% in radiographs and 94.2% in CT, and all fractures were detected in MRI. MRI showed a more complex fracture pattern compared with CT in 65% of the cases. We observed 71.4% of single SIFs, 9.1% with other spinal fractures, 13.0% with other pelvic fractures, and 7.8% with other fractures. According to the SIF fracture pattern, the H/U type was 40.2%, transverse type was 33.7%, λ/T type was 24.7%, unilateral vertical type was 1.3%, and bilateral vertical type was 0%. Conclusions: an MRI of the lumbar spine including the sacrum with a coronal fat-suppressed T2-weighted image is useful for elderly patients with suddenly increasing low back pain at an early stage. This procedure improves an early SIF detection, recognition of concomitant pathologies, and adequate treatment for the patients.
New and Upcoming Topical Treatments for Atopic Dermatitis: A Review of the Literature
Authors: Sideris Nikolaos , Paschou Eleni , Bakirtzi Katerina , Kiritsi Dimitra , Papadimitriou Ilias , Tsentemeidou Aikaterini , Sotiriou Elena , Vakirlis Efstratios
Published: August 2022

Atopic dermatitis (AD) is a chronic inflammatory dermatosis with periods of exacerbation and remissions. AD is characterized by intense, persistent pruritus and heterogeneity in clinical symptomatology and severity. Therapeutic goals include the amelioration of cutaneous eruptions, diminishing relapses and eventually the disease burden. To date, topical corticosteroids (TCS) and calcineurin inhibitors (TCI) have yet been deemed the mainstay of topical treatments in AD management. Nevertheless, despite their indisputable efficiency, TCS and TCI are not indicated for continuous long-term use given their safety profile. While research in AD has concentrated predominantly on systemic therapies, more than 30 novel topical compounds are under development. The existing data appear encouraging, with some regimens that are already FDA-approved (ruxolitinib was the most recent in September 2021) and several pharmaceutical pipeline products for mild-to-moderate AD that are in an advanced stage of development, such as tapinarof, difamilast and roflumilast. Larger, long-term studies are still required to evaluate the efficacy and safety of these novel compounds in the long run and weigh their advantages over present treatments. In this review, we aim to provide an overview of the latest knowledge about AD topical treatments, echoing upcoming research trends.
Shortening the Biliopancreatic Limb Length of One Anastomosis Gastric Bypass Maintains Glucose Homeostasis Improvement with Limited Weight Loss
Authors: Ribeiro-Parenti Lara , El Jindi Hounayda , Willemetz Alexandra , Siebert Matthieu , Kapel Nathalie , Le Beyec Johanne , Bado André , Le Gall Maude
Published: August 2022

One anastomosis gastric bypass (OAGB) is associated with similar metabolic improvements and weight loss as Roux-en-Y gastric bypass (RYGB). However, this bariatric procedure is still controversial as it is suspected to result in undernutrition. Reducing the size of the biliopancreatic limb of OAGB could be essential to maintain positive outcomes while preventing side effects. The objective of this study was to compare and contrast outcomes of OAGB with two different biliopancreatic limb lengths to RYGB and Sham surgery in obese and non-obese rats. Lean and diet-induced obese Wistar rats were operated on RYGB, OAGB with a short (15 cm OAGB-15) or a long (35 cm OAGB-35) biliopancreatic limb or Sham surgery. Body weight and food intake were monitored over 30 weeks, and rats underwent oral glucose and insulin tolerance tests with a pancreatic and gut hormone secretion assay. Macronutrient absorption was determined by fecal analyses. Statistical analyses used non-parametric one-way or two-way ANOVA tests. Compared to Sham rats, RYGB, OAGB-15 and OAGB-35 rats displayed a significant reduced weight. Weight loss was greater after OAGB-35 than after OAGB-15 or Sham surgery because of transient malabsorption. All OAGB- and RYGB-operated rats displayed an improved pancreatic and gut hormone secretion in response to a meal compared to Sham rats, these effects were independent of limb length, rat weight, and maintained overtime. In conclusion, glucose homeostasis was similarly improved in obese and non-obese OAGB-15 and OAGB-35 rats suggesting that shortening the biliopancreatic limb can improve the metabolic parameters without a major influence on weight.
Impact of the Volume and Distribution of Training Datasets in the Development of Deep-Learning Models for the Diagnosis of Colorectal Polyps in Endoscopy Images
Authors: Gong Eun Jeong , Bang Chang Seok , Lee Jae Jun , Yang Young Joo , Baik Gwang Ho
Published: August 2022

Background: Establishment of an artificial intelligence model in gastrointestinal endoscopy has no standardized dataset. The optimal volume or class distribution of training datasets has not been evaluated. An artificial intelligence model was previously created by the authors to classify endoscopic images of colorectal polyps into four categories, including advanced colorectal cancer, early cancers/high-grade dysplasia, tubular adenoma, and nonneoplasm. The aim of this study was to evaluate the impact of the volume and distribution of training dataset classes in the development of deep-learning models for colorectal polyp histopathology prediction from endoscopic images. Methods: The same 3828 endoscopic images that were used to create earlier models were used. An additional 6838 images were used to find the optimal volume and class distribution for a deep-learning model. Various amounts of data volume and class distributions were tried to establish deep-learning models. The training of deep-learning models uniformly used no-code platform Neuro-T. Accuracy was the primary outcome on four-class prediction. Results: The highest internal-test classification accuracy in the original dataset, doubled dataset, and tripled dataset was commonly shown by doubling the proportion of data for fewer categories (2:2:1:1 for advanced colorectal cancer: early cancers/high-grade dysplasia: tubular adenoma: non-neoplasm). Doubling the proportion of data for fewer categories in the original dataset showed the highest accuracy (86.4%, 95% confidence interval: 85.0–97.8%) compared to that of the doubled or tripled dataset. The total required number of images in this performance was only 2418 images. Gradient-weighted class activation mapping confirmed that the part that the deep-learning model pays attention to coincides with the part that the endoscopist pays attention to. Conclusion: As a result of a data-volume-dependent performance plateau in the classification model of colonoscopy, a dataset that has been doubled or tripled is not always beneficial to training. Deep-learning models would be more accurate if the proportion of fewer category lesions was increased.
Position Classification of the Endotracheal Tube with Automatic Segmentation of the Trachea and the Tube on Plain Chest Radiography Using Deep Convolutional Neural Network
Authors: Jung Heui Chul , Kim Changjin , Oh Jaehoon , Kim Tae Hyun , Kim Beomgyu , Lee Juncheol , Chung Jae Ho , Byun Hayoung , Yoon Myeong Seong , Lee Dong Keon
Published: August 2022

Background: This study aimed to develop an algorithm for multilabel classification according to the distance from carina to endotracheal tube (ETT) tip (absence, shallow > 70 mm, 30 mm ≤ proper ≤ 70 mm, and deep position < 30 mm) with the application of automatic segmentation of the trachea and the ETT on chest radiographs using deep convolutional neural network (CNN). Methods: This study was a retrospective study using plain chest radiographs. We segmented the trachea and the ETT on images and labeled the classification of the ETT position. We proposed models for the classification of the ETT position using EfficientNet B0 with the application of automatic segmentation using Mask R-CNN and ResNet50. Primary outcomes were favorable performance for automatic segmentation and four-label classification through five-fold validation with segmented images and a test with non-segmented images. Results: Of 1985 images, 596 images were manually segmented and consisted of 298 absence, 97 shallow, 100 proper, and 101 deep images according to the ETT position. In five-fold validations with segmented images, Dice coefficients [mean (SD)] between segmented and predicted masks were 0.841 (0.063) for the trachea and 0.893 (0.078) for the ETT, and the accuracy for four-label classification was 0.945 (0.017). In the test for classification with 1389 non-segmented images, overall values were 0.922 for accuracy, 0.843 for precision, 0.843 for sensitivity, 0.922 for specificity, and 0.843 for F1-score. Conclusions: Automatic segmentation of the ETT and trachea images and classification of the ETT position using deep CNN with plain chest radiographs could achieve good performance and improve the physician’s performance in deciding the appropriateness of ETT depth.
Geospatial Analysis of Nonmarket Values to Prioritize Forest Restoration
Authors: Soder Adrienne B. , Mueller Julie M. , Springer Abraham E. , LaPine Katelyn E.
Published: August 2022

Forest restoration is necessary for maintaining healthy watersheds and the ecological spatial networks that provide environmental goods and services. Consideration of the dollar value of these provided benefits in restoration planning is essential to the efficient use of limited resources available to project implementation. Nonmarket valuation is a methodology of economics commonly used to estimate monetary values for environmental goods and services that are not typically bought or sold in a traditional market. Valuation studies are prolific within the restoration literature; however, the use of nonmarket values as decision support is not well represented. We introduce a method using Geographic Information Systems (GIS) to spatially analyze the results from a nonmarket valuation study that estimated dollar values for the attributes of forest restoration characteristic of a semi-arid watershed in the Southwest United States. Map layers were created for the five attributes valued by the study and represent areas in the watershed that are designated as critical habitats, determined to influence surface water quality, prone to high-severity wildfire, representative of culturally significant areas, and contribute to aquifer recharge. A series of overlay analyses were performed to create a composite benefit map that spatially displays nonmarket values throughout the watershed. The per acre benefit values range from USD 0 to USD 104 where all five attributes are present.
Causes and Countermeasures for the Failure of Mining Land Use Policy Reform: Practice Analysis from China
Authors: Guo Jiaxin , Hu Zhenqi , Liang Yusheng
Published: August 2022

The current supply method of land acquisition for mining land in China is not conducive to the sustainable use of land resources, resulting in a large amount of wasted land resources and causing many conflicts. The new model of temporary land use policy for mining solves the long-standing problem of the livelihood of expropriated farmers that has plagued local governments, and also helps to alleviate the conflicts between enterprises and farmers. However, the temporary land use policy is in the practice stage, and the policy failure has resulted in low land reclamation rates. This research is a systematic survey for large and medium-sized mining enterprises, relevant government departments and research institutions nationwide. From the survey we analyze the problems in the implementation of the current temporary land use policy for mining and the causes of the policy failure, and propose an effective implementation mechanism for the future policy. The study showed that: (1) the temporary land use policy for mining was enacted with wide acceptance, but the implementation of the policy was ineffective; (2) the conditions for the application of the temporary land use policy for mining are unclear, the review and supervision by the competent authorities are not strict, and enterprises do not pay attention to land reclamation resulting in a very low rate of land reclamation; and (3) The implementation mechanism of the temporary mining land policy in practice is not perfect, and the proposed implementation framework based on “conditions, approval, implementation, supervision, acceptance and withdrawal” is scientific and feasible. It provides a reference for the management and innovation of strict protection of arable land, land conservation and intensification, and land reclamation in mining areas in China.
Amor Fati: On ‘Crimes of Passion’ in Portuguese Law
Authors: Oliveira Ana
Published: August 2022

The timelessness of the matters of love and heartbreak is evident from the place that these themes have historically held in the literature. Fictional representations of love and estrangement are frequently recovered within legal reasoning, because of the nature of the stories portrayed, or the ethical-normative judgements and frames of reference on which their literary enunciation is based. In the field of law, the formal structuring of these matters and its penal relevance draw on ‘crimes of passion’ as an example and a sign of the legal conditions, interpretative constructs, and sociological conceptions that organize and give meaning to subjects, facts, and norms. Whether it is the cause that justifies the fact, a mitigating factor that modifies the crime or punishment, or a particularly reprehensible and perverse circumstance, this ‘crazy little thing called love’ has provoked and shaped different levels of censure and comprehension throughout history. That very elasticity is the starting point for this article, which examines the legal frameworks and the legal, literary, and historical imaginations that circulate and connect diverse interpretative communities, as well as the discursive debates over authority and normativity, in the different fictions and functions linked by their aspiration to truth and justice.
Interaction of the Blood Components with Ascending Thoracic Aortic Aneurysm Wall: Biomechanical and Fluid Analyses
Authors: Taheri Ramezan Ali , Razaghi Reza , Bahramifar Ali , Morshedi Mahdi , Mafi Majid , Karimi Alireza
Published: August 2022

Background: Ascending thoracic aortic aneurysm (ATAA) is an asymptomatic localized dilation of the aorta that is prone to rupture with a high rate of mortality. While diameter is the main risk factor for rupture assessment, it has been shown that the peak wall stress from finite element (FE) simulations may contribute to refinement of clinical decisions. In FE simulations, the intraluminal boundary condition is a single-phase blood flow that interacts with the thoracic aorta (TA). However, the blood is consisted of red blood cells (RBCs), white blood cells (WBCs), and plasma that interacts with the TA wall, so it may affect the resultant stresses and strains in the TA, as well as hemodynamics of the blood. Methods: In this study, discrete elements were distributed in the TA lumen to represent the blood components and mechanically coupled using fluid–structure interaction (FSI). Healthy and aneurysmal human TA tissues were subjected to axial and circumferential tensile loadings, and the hyperelastic mechanical properties were assigned to the TA and ATAA FE models. Results: The ATAA showed larger tensile and shear stresses but smaller fluid velocity compared to the ATA. The blood components experienced smaller shear stress in interaction with the ATAA wall compared to TA. The computational fluid dynamics showed smaller blood velocity and wall shear stress compared to the FSI. Conclusions: This study is a first proof of concept, and future investigations will aim at validating the novel methodology to derive a more reliable ATAA rupture risk assessment considering the interaction of the blood components with the TA wall.
Exome-Wide Association Study Reveals Host Genetic Variants Likely Associated with the Severity of COVID-19 in Patients of European Ancestry
Authors: Upadhyai Priyanka , Shenoy Pooja U. , Banjan Bhavya , Albeshr Mohammed F. , Mahboob Shahid , Manzoor Irfan , Das Ranajit
Published: August 2022

Host genetic variability plays a pivotal role in modulating COVID-19 clinical outcomes. Despite the functional relevance of protein-coding regions, rare variants located here are less likely to completely explain the considerable numbers of acutely affected COVID-19 patients worldwide. Using an exome-wide association approach, with individuals of European descent, we sought to identify common coding variants linked with variation in COVID-19 severity. Herein, cohort 1 compared non-hospitalized (controls) and hospitalized (cases) individuals, and in cohort 2, hospitalized subjects requiring respiratory support (cases) were compared to those not requiring it (controls). 229 and 111 variants differed significantly between cases and controls in cohorts 1 and 2, respectively. This included FBXO34, CNTN2, and TMCC2 previously linked with COVID-19 severity using association studies. Overall, we report SNPs in 26 known and 12 novel candidate genes with strong molecular evidence implicating them in the pathophysiology of life-threatening COVID-19 and post-recovery sequelae. Of these few notable known genes include, HLA-DQB1, AHSG, ALOX5AP, MUC5AC, SMPD1, SPG7, SPEG,GAS6, and SERPINA12. These results enhance our understanding of the pathomechanisms underlying the COVID-19 clinical spectrum and may be exploited to prioritize biomarkers for predicting disease severity, as well as to improve treatment strategies in individuals of European ancestry.
Analysis of a Low-Loss Gear Geometry Using a Thermal Elastohydrodynamic Simulation including Mixed Lubrication
Authors: Farrenkopf Felix , Schwarz Andreas , Lohner Thomas , Stahl Karsten
Published: August 2022

Low-loss gears are an interesting design approach for increasing the efficiency and thermal load limits of gearboxes. The loss-optimized gear geometry concentrates sliding around the pitch point, which results in low load-dependent gear power losses. In this study, a method for modeling transient EHL (elastohydrodynamically lubricated) contacts in gear mesh considering mixed lubrication and thermal effects is introduced and applied to analyze the tribological behavior of a low-loss gear geometry. Special focus is placed on local frictional losses to analyze the role of the thermal effects of the gear mesh. Although a thermal reduction in fluid friction is observed, the overall effect on total frictional losses of the low-loss gear geometry is evaluated to be very small. The edge geometry strongly influences the lubricant film thickness and frictional power losses.
Phase Separation in a Novel Selective Lithium Extraction from Citrate Media with D2EHPA
Authors: Punt Tiaan , Bradshaw Steven M. , Van Wyk Petrie , Akdogan Guven
Published: August 2022

Lithium-ion battery (LIB) recycling has received continued interest in recent years due to its benefits, which include reducing the environmental impact of spent LIBs and providing a secondary source of valuable metals, such as Li, Co, and Ni. This paper characterized the Li separation with D2EHPA from citrate media as a function of pH and identified the optimal overall Li separation at a pH of 5.5. The Li separation was optimized at a pH of 5.5, with which it was concluded that 23 vol.% D2EHPA and an O/A ratio of 4 provided the best Li separation, for which 66.1% Li was extracted with 26.9% residual Mn, 6.8% Co, and 7.7% Ni in a single stage. The formation of a reversible hydrophobic third phase was identified during Li extraction at a pH of 5.5 or greater. Investigation of the third phase revealed that more than 99% of the Li, Co, and Ni were extracted to the third phase, while more than 69% of the Mn was extracted to the organic phase. STEM images of the third phase revealed a honeycomb-like structure, which was hypothesized to be a 2D mesoporous film caused by the insolubility of the organometallic complexes in the aqueous and organic phase.
Updates and Original Case Studies Focused on the NMR-Linked Metabolomics Analysis of Human Oral Fluids Part II: Applications to the Diagnosis and Prognostic Monitoring of Oral and Systemic Cancers
Authors: Grootveld Martin , Percival Benita C. , Page Georgina , Hunwin Kayleigh , Bhogadia Mohammed , Chan Wyman , Edgar Mark
Published: August 2022

Human saliva offers many advantages over other biofluids regarding its use and value as a bioanalytical medium for the identification and prognostic monitoring of human diseases, mainly because its collection is largely non-invasive, is relatively cheap, and does not require any major clinical supervision, nor supervisory input. Indeed, participants donating this biofluid for such purposes, including the identification, validation and quantification of surrogate biomarkers, may easily self-collect such samples in their homes following the provision of full collection details to them by researchers. In this report, the authors have focused on the applications of metabolomics technologies to the diagnosis and progressive severity monitoring of human cancer conditions, firstly oral cancers (e.g., oral cavity squamous cell carcinoma), and secondly extra-oral (systemic) cancers such as lung, breast and prostate cancers. For each publication reviewed, the authors provide a detailed evaluation and critical appraisal of the experimental design, sample size, ease of sample collection (usually but not exclusively as whole mouth saliva (WMS)), their transport, length of storage and preparation for analysis. Moreover, recommended protocols for the optimisation of NMR pulse sequences for analysis, along with the application of methods and techniques for verifying and resonance assignments and validating the quantification of biomolecules responsible, are critically considered. In view of the authors’ specialisms and research interests, the majority of these investigations were conducted using NMR-based metabolomics techniques. The extension of these studies to determinations of metabolic pathways which have been pathologically disturbed in these diseases is also assessed here and reviewed. Where available, data for the monitoring of patients’ responses to chemotherapeutic treatments, and in one case, radiotherapy, are also evaluated herein. Additionally, a novel case study featured evaluates the molecular nature, levels and diagnostic potential of 1H NMR-detectable salivary ‘acute-phase’ glycoprotein carbohydrate side chains, and/or their monomeric saccharide derivatives, as biomarkers for cancer and inflammatory conditions.
Influence of Genetic West African Ancestry on Metabolomics among Hypertensive Patients
Authors: Mehanna Mai , McDonough Caitrin W. , Smith Steven M. , Gong Yan , Gums John G. , Chapman Arlene B. , Johnson Julie A. , Cooper-DeHoff Rhonda M.
Published: August 2022

Patients with higher genetic West African ancestry (GWAA) have hypertension (HTN) that is more difficult to treat and have higher rates of cardiovascular diseases (CVD) and differential responses to antihypertensive drugs than those with lower GWAA. The mechanisms underlying these disparities are poorly understood. Using data from 84 ancestry-informative markers in US participants from the Pharmacogenomic Evaluation of Antihypertensive Responses (PEAR) and PEAR-2 trials, the GWAA proportion was estimated. Using multivariable linear regression, the baseline levels of 886 metabolites were compared between PEAR participants with GWAA < 45% and those with GWAA ≥ 45% to identify differential metabolites and metabolic clusters. Metabolites with a false discovery rate (FDR) < 0.2 were used to create metabolic clusters, and a cluster analysis was conducted. Differential clusters were then tested for replication in PEAR-2 participants. We identified 353 differential metabolites (FDR < 0.2) between PEAR participants with GWAA < 45% (n = 383) and those with GWAA ≥ 45% (n = 250), which were used to create 24 metabolic clusters. Of those, 13 were significantly different between groups (Bonferroni p < 0.002). Four clusters, plasmalogen and lysoplasmalogen, sphingolipid metabolism and ceramide, cofactors and vitamins, and the urea cycle, were replicated in PEAR-2 (Bonferroni p < 0.0038) and have been previously linked to HTN and CVD. Our findings may give insights into the mechanisms underlying HTN racial disparities.
Utilization Methods and Practice of Abandoned Mines and Related Rock Mechanics under the Ecological and Double Carbon Strategy in China—A Comprehensive Review
Authors: Du Kun , Xie Junjie , Khandelwal Manoj , Zhou Jian
Published: August 2022

Governance of abandoned mines has become a pressing issue for China. The utilization of abandoned mines is a technology that can solve the problem of governance and recreate the value of mines, which is in line with the current strategic goals of ecological protection and double carbon in China. In this paper, the various utilization models and the advances in rock mechanics of abandoned mines across the globe are summarized and reviewed. The utilization models of abandoned mines can be categorized into four aspects: Energy storage, Waste treatment, Ecological restoration, and carbon dioxide (CO2) sequestration. There are a number of applications and uses of abandoned mines, such as pumped storage, compressed air storage, salt cavern gas/oil storage construction, carbon dioxide storage and utilization, radioactive waste disposal and treatment, and tourism development. Various progress practices of abandoned mines are discussed in detail with emphasis on the national conditions of China. The basic rock mechanics problems and advances involved in the construction of the facilities related to the utilization of abandoned mines are discussed and evaluated. The establishment of relevant research and experimental platforms will contribute to the sustainable development of China’s mining industry and the improvement of clean technologies.
Hydrodeoxygenation of Levulinic Acid to γ-Valerolactone over Mesoporous Silica-Supported Cu-Ni Composite Catalysts
Authors: Popova Margarita , Trendafilova Ivalina , Oykova Manuela , Mitrev Yavor , Shestakova Pavleta , Mihályi Magdolna R. , Szegedi Ágnes
Published: August 2022

Monometallic (Cu, Ni) and bimetallic (Cu-Ni) catalysts supported on KIT-6 based mesoporous silica/zeolite composites were prepared using the wet impregnation method. The catalysts were characterized using X-ray powder diffraction, N2 physisorption, SEM, solid state NMR and H2-TPR methods. Finely dispersed NiO and CuO were detected after the decomposition of impregnating salt on the silica carrier. The formation of small fractions of ionic Ni2+ and/or Cu2+ species, interacting strongly with the silica supports, was found. The catalysts were studied in the gas-phase upgrading of lignocellulosic biomass-derived levulinic acid (LA) to γ-valerolactone (GVL). The bimetallic, CuNi-KIT-6 catalyst showed 100% LA conversion at 250 °C and atmospheric pressure. The high LA conversion and GVL yield can be attributed to the high specific surface area and finely dispersed Cu-Ni species in the catalyst. Furthermore, the catalyst also exhibited high stability after 24 h of reaction time with a GVL yield above 80% without any significant change in metal dispersion.
Volatile Composition and Sensory Profile of Lactose-Free Kefir, and Its Acceptability by Elderly Consumers
Authors: Rutkowska Jaroslawa , Antoniewska-Krzeska Agata , Żbikowska Anna , Cazón Patricia , Vázquez Manuel
Published: August 2022

Lactose-free products are crucial in the diet of lactose-intolerant elderly consumers, one of them being kefir due to its unique chemical composition and diversity of valuable microflora. The study aimed at determining the volatile compound profile and the corresponding sensory attributes of lactose-free kefir (LFK) as compared with the traditional one (TK). The perception of main sensory attributes and hedonic acceptability of LFK by elderly were also studied. The LFK contained two times more ketones, especially 3-hydroxy-2-butanone and 2,3-butanedione, that probably contributed to the high intensity of creamy aroma. A substantial share of acetic acid in LFK was not associated with high intensity of sour aroma, probably being masked by the creamy aroma, perceived as dominating. LFK was sensed as sweeter and more milky than the traditional one. The intense sweet taste of LFK was due to higher amounts of glucose and galactose than in TK, and was perceived as “just about right” by 63% of elderly subjects in the just-about-right (JAR) scale. The lower acidity of LFK than that of TK, assayed both instrumentally and by sensory assessment, was highly appreciated by 73% of elderly subjects as “just about right” in JAR scale. These two taste attributes dominated in liking the lactose-free kefir by elderly subjects.
Structural Investigation of Hesperetin-7-O-Glucoside Inclusion Complex with β-Cyclodextrin: A Spectroscopic Assessment
Authors: Kapoor Mahendra P. , Moriwaki Masamitsu , Minoura Katsuhiko , Timm Derek , Abe Aya , Kito Kento
Published: August 2022

Flavonoids are biologically active natural products of great interest for their potential applications in functional foods and pharmaceuticals. A hesperetin-7-O-glucoside inclusion complex with β-cyclodextrin (HEPT7G/βCD; SunActive® HCD) was formulated via the controlled enzymatic hydrolysis of hesperidin with naringinase enzyme. The conversion rate was nearly 98%, estimated using high-performance liquid chromatography analysis. The objective of this study was to investigate the stability, solubility, and spectroscopic features of the HEPT7G/βCD inclusion complex using Fourier-transform infrared (FTIR), Raman, ultraviolet–visible absorption (UV–vis), 1H- and 13C- nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC), liquid chromatography/mass spectroscopy (LC–MS), scanning electron microscopy (SEM), and powdered X-ray diffraction (PXRD) spectroscopic techniques including zeta potential, Job’s plot, and phase solubility measurements. The effects of complexation on the profiles of supramolecular interactions in analytic features, especially the chemical shifts of β-CD protons in the presence of the HEPT7G moiety, were evaluated. The stoichiometric ratio, stability, and solubility constants (binding affinity) describe the extent of complexation of a soluble complex in 1:1 stoichiometry that exhibits a greater affinity and fits better into the β-CD inner cavity. The NMR spectroscopy results identified two different configurations of the HEPT7G moiety and revealed that the HEPT7G/βCD inclusion complex has both –2S and –2R stereoisomers of hesperetin-7-O-glucoside possibly in the –2S/–2R epimeric ratio of 1/1.43 (i.e., –2S: 41.1% and –2R: 58.9%). The study indicated that encapsulation of the HEPT7G moiety in β-CD is complete inclusion, wherein both ends of HEPT7G are included in the β-CD inner hydrophobic cavity. The results showed that the water solubility and thermal stability of HEPT7G were apparently increased in the inclusion complex with β-CD. This could potentially lead to increased bioavailability of HEPT7G and enhanced health benefits of this flavonoid.
Biocatalysts Synthesized with Lipase from Pseudomonas cepacia on Glycol-Modified ZIF-8: Characterization and Utilization in the Synthesis of Green Biodiesel
Authors: Martínez Gil José Manuel , Vivas Reyes Ricardo , Bastidas Barranco Marlon José , Giraldo Liliana , Moreno-Piraján Juan Carlos
Published: August 2022

This research presents results on the production of biodiesel from the transesterification of acylglycerides present in palm oil, using the biocatalysts ZIF-8-PCL and Gly@ZIF-8-PCL synthesized by immobilization of Pseudomonas Cepacia Lipase as catalytic materials and using pure ZIF-8 and Gly@ZIF-8 (modified ZIF-8) as supports. The Gly@ZIF-8 carbonaceous material was prepared by wet impregnation of ZIF-8 with ethylene glycol as the carbon source, and then thermally modified. The calcination conditions were 900 °C for two hours with a heating rate of 7 °C/min in an inert atmosphere. A textural characterization was performed, and results showed superficial changes of materials at the microporous and mesoporous levels for the Gly@ZIF-8 material. Both the starting materials and biocatalysts were characterized by infrared spectroscopy (FTIR) and Raman spectroscopy. During the transesterification, using the two biocatalysts (ZIF-8-PCL and Gly@ZIF-8-PCL), two supernatant liquids were generated which were characterized by infrared spectroscopy (FTIR), gas chromatography coupled to mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The results show that the two routes of synthesis of supports from ZIF-8 will be configured as effective methods for the generation of effective biocatalysts for biodiesel production.
Bridging a Century-Old Problem: The Pathophysiology and Molecular Mechanisms of HA Filler-Induced Vascular Occlusion (FIVO)—Implications for Therapeutic Interventions
Authors: Soares Danny
Published: August 2022

Biocompatible hyaluronic acid (HA, hyaluronan) gel implants have altered the therapeutic landscape of surgery and medicine, fostering an array of innovative products that include viscosurgical aids, synovial supplements, and drug-eluting nanomaterials. However, it is perhaps the explosive growth in the cosmetic applications of injectable dermal fillers that has captured the brightest spotlight, emerging as the dominant modality in plastic surgery and aesthetic medicine. The popularity surge with which injectable HA fillers have risen to in vogue status has also brought a concomitant increase in the incidence of once-rare iatrogenic vaso-occlusive injuries ranging from disfiguring facial skin necrosis to disabling neuro-ophthalmological sequelae. As our understanding of the pathophysiology of these injuries has evolved, supplemented by more than a century of astute observations, the formulation of novel therapeutic and preventative strategies has permitted the amelioration of this burdensome complication. In this special issue article, we review the relevant mechanisms underlying HA filler-induced vascular occlusion (FIVO), with particular emphasis on the rheo-mechanical aspects of vascular blockade; the thromboembolic potential of HA mixtures; and the tissue-specific ischemic susceptibility of microvascular networks, which leads to underperfusion, hypoxia, and ultimate injury. In addition, recent therapeutic advances and novel considerations on the prevention and management of muco-cutaneous and neuro-ophthalmological complications are examined.