bluuebunny/crossref_metadata_2025 huggingface dataset:
The four-dimensional Yang–Mills partition function in the vicinity of the vacuum
Authors: Etesi Gábor
Published: April 2023

The partition function of four-dimensional Euclidean, non-supersymmetric$$\textrm{SU}(2)$$SU(2)Yang–Mills theory is calculated in the perturbative and weak coupling regime, i.e., in a small open ball about the flat connection and when the gauge coupling constant acquires a small but finite value. The computation is based on various known inequalities, valid only in four dimensions, providing two-sided estimates for the exponentiated Yang–Mills action in terms of the$$L^2$$L2-norm of the derivative of the gauge potential only; these estimates then give rise to Gaußian-like infinite- dimensional formal integrals involving the Laplacian and hence can be computed via zeta-function and heat kernel techniques. It then turns out that these formal integrals give a sharp value for the partition function in the aforementioned perturbative and weak coupling regime of the theory. In the resulting expression for the partition function, the original classical value of the coupling constant is shifted to a smaller one which can be interpreted as the manifestation, in this approach, of a non-trivial$$\beta $$β-function and asymptotic freedom in pure non-Abelian gauge theories.
An empirical study of quantum dynamics as a ground state problem with neural quantum states
Authors: Vargas-Calderón Vladimir , Vinck-Posada Herbert , González Fabio A.
Published: April 2023

We consider the Feynman–Kitaev formalism applied to a spin chain described by the transverse-field Ising model. This formalism consists of building a Hamiltonian whose ground state encodes the time evolution of the spin chain at discrete time steps. To find this ground state, variational wave functions parameterised by artificial neural networks—also known as neural quantum states (NQSs)—are used. Our work focuses on assessing, in the context of the Feynman–Kitaev formalism, two properties of NQSs: expressivity (the possibility that variational parameters can be set to values such that the NQS is faithful to the true ground state of the system) and trainability (the process of reaching said values). We find that the considered NQSs are capable of accurately approximating the true ground state of the system, i.e. they are expressive enough ansätze. However, extensive hyperparameter tuning experiments show that, empirically, reaching the set of values for the variational parameters that correctly describe the ground state becomes ever more difficult as the number of time steps increase because the true ground state becomes more entangled, and the probability distribution starts to spread across the Hilbert space canonical basis.
What are teacher relational skills? A defining study using a bottom-up modified Delphi method
Authors: Gerosa Tiziano , Argentin Gianluca , Spada Alice
Published: April 2023

Relational skills are an essential work-related tool for several professions, especially teaching. This specific domain of teacher competence is acquiring ever-increasing attention due to the connection between social and emotional aspects of education and student school success. Nonetheless, a persistent focus on content-related knowledge, didactic skills and digital literacy has generated uncertainties about what teacher relational skills effectively are. In this respect, our study presents an alternative bottom-up approach for the definition of teacher relational skills based on the use of a modified Delphi method. Four iterative rounds of data collection and analysis were carried out on a sample of 35 experienced teachers. This Delphi was pushed to generate innovative definitions, taking advantage of its bottom up perspective with teachers simultaneously involved as reflective professionals and as experts by experience of professional development completion. The emerging innovative elements were coherently integrated with previous literature thanks to a circular relationship between the Delphi panelists and an external group of experts. As a result, 6 relational domains, 44 relational skills and 180 practical examples of their application were identified. Our findings underline the importance of stimulating the debate around direct experiences of effective practices for the development of competence-based working approaches, especially in the areas of socialization, relations and emotions, where well established theoretical frameworks are still a long way off.
A Republican Approach to Jerkish Speech on Online Platforms
Authors: Hoeksema Bernd
Published: April 2023

Jerkish speech on online platforms is at risk of being overlooked as a result of being comparatively insignificant next to the existence of explicit hate speech or other online harms. In this paper I approach online jerkish speech from a republican perspective. I discuss two ways in which republicans can account for jerkish speech. First, jerkish speech could amount to micro-domination, referring to instances of domination that are relatively inconsequential by themselves but problematic when considered in aggregate. Second, jerkish speech can be considered an instantiation of systemic domination, where groups and members are structurally subordinated. I briefly discuss the role of regulatory bans on jerkish speech, before suggesting that Suzanne Whitten’s proposal for critical civility—as an appropriate answer to jerkish speech in online environments—might be too demanding. This might be alleviated by leveraging the role of online platforms.
Alpha band modulation caused by selective attention to music enables EEG classification
Authors: Mizokuchi Kana , Tanaka Toshihisa , Sato Takashi G. , Shiraki Yoshifumi
Published: April 2023

Humans are able to pay selective attention to music or speech in the presence of multiple sounds. It has been reported that in the speech domain, selective attention enhances the cross-correlation between the envelope of speech and electroencephalogram (EEG) while also affecting the spatial modulation of the alpha band. However, when multiple music pieces are performed at the same time, it is unclear how selective attention affects neural entrainment and spatial modulation. In this paper, we hypothesized that the entrainment to the attended music differs from that to the unattended music and that spatial modulation in the alpha band occurs in conjunction with attention. We conducted experiments in which we presented musical excerpts to 15 participants, each listening to two excerpts simultaneously but paying attention to one of the two. The results showed that the cross-correlation function between the EEG signal and the envelope of the unattended melody had a more prominent peak than that of the attended melody, contrary to the findings for speech. In addition, the spatial modulation in the alpha band was found with a data-driven approach called the common spatial pattern method. Classification of the EEG signal with a support vector machine identified attended melodies and achieved an accuracy of 100% for 11 of the 15 participants. These results suggest that selective attention to music suppresses entrainment to the melody and that spatial modulation of the alpha band occurs in conjunction with attention. To the best of our knowledge, this is the first report to detect attended music consisting of several types of music notes only with EEG.
Does Inflammation Play a Major Role in the Pathogenesis of Alzheimer's Disease?
Authors: Wiatrak Benita , Jawień Paulina , Szeląg Adam , Jęśkowiak-Kossakowska Izabela
Published: April 2023

Alzheimer's disease (AD) is a neurodegenerative disease leading to dementia for which no effective medicine exists. Currently, the goal of therapy is only to slow down the inevitable progression of the disease and reduce some symptoms. AD causes the accumulation of proteins with the pathological structure of Aβ and tau and the induction of inflammation of nerves in the brain, which lead to the death of neurons. The activated microglial cells produce pro-inflammatory cytokines that induce a chronic inflammatory response and mediate synapse damage and the neuronal death. Neuroinflammation has been an often ignored aspect of ongoing AD research. There are more and more scientific papers taking into account the aspect of neuroinflammation in the pathogenesis of AD, although there are no unambiguous results regarding the impact of comorbidities or gender differences. This publication concerns a critical look at the role of inflammation in the progression of AD, based on the results of our own in vitro studies using model cell cultures and other researchers.
Impact of perineural invasion on the outcome of patients with synchronous colorectal liver metastases treated with neoadjuvant chemotherapy and surgery
Authors: Zozaya Gabriel , Cienfuegos Javier Álvarez , Martí-Cruchaga Pablo , Hernández-Lizoain José Luis , Baixauli Jorge , Pardo Fernando , Robledano Ramón , Rodríguez Javier , Arbea Leire , Rotellar Fernando
Published: April 2023

Purpose

To analyze the prognostic value of variables of the primary tumor in patients with synchronous liver metastases in colorectal cancer (CLRMs) treated with neoadjuvant chemotherapy and surgery.

Methods/Patients

From a prospective database, we retrospectively identified all patients with synchronous CLRMs who were treated with neoadjuvant chemotherapy and liver resection. Using univariate and multivariate analyses, we identified the variables associated with tumor recurrence. Overall survival and disease-free survival were calculated using the Kaplan–Meier method with differences determined by the Cox multiple hazards model. Results were compared using the log-rank test.

Results

Ninety-eight patients with synchronous CLRMs were identified. With a median follow-up of 39.8 months, overall survival and disease-free survival at 5 and 10 years were 53%, 41.7%, 29% and 29%, respectively. Univariate analysis identified three variables associated with tumor recurrence: location in the colon (p = 0.025), lymphovascular invasion (p = 0.011) and perineural invasion (p = 0.005). Multivariate analysis identified two variables associated with worse overall survival: perineural invasion (HR 2.36, 95% CI 1.162–4.818, p = 0.018) and performing frontline colectomy (HR 3.286, 95% CI 1.256–8.597, p = 0.015). Perineural invasion remained as the only variable associated with lower disease-free survival (HR 1.867, 95% CI 1.013–3.441, p = 0.045). Overall survival at 5 and 10 years in patients with and without perineural invasion was 68.2%, 54.4% and 29.9% and 21.3%, respectively (HR 5.920, 95% CI 2.241–15.630, p < 0.001).

Conclusions

Perineural invasion in the primary tumor is the variable with most impact on survival in patients with synchronous CLRMs treated with neoadjuvant chemotherapy and surgery.
Observational study of HR+/HER2− metastatic breast cancer patients treated with abemaciclib in Spain in the Named Patient Use Program (AbemusS)
Authors: Blanch Salvador , Gil-Gil Juan Miguel , Arumí Miriam , Aguirre Elena , Seguí Miguel Ángel , Atienza Manuel , Díaz-Cerezo Silvia , Molero Alberto , Cervera José Manuel , Gavilá Joaquín
Published: April 2023

Introduction/objectives

To describe abemaciclib use in patients with hormone receptor-positive, human epidermal growth factor receptor-negative (HR+/HER2−) metastatic breast cancer (mBC) who participated in the Named Patient Use program (NPU) in Spain.

Material and methods

This retrospective study was based on medical record review of patients across 20 centers during 2018/2019. Patients were followed up until death, enrolment in a clinical trial, loss of follow-up or study end. Clinical and demographic characteristics, treatment patterns and abemaciclib effectiveness were analyzed; time-to-event and median times were estimated using the Kaplan–Meier (KM) method.

Results

The study included 69 female patients with mBC (mean age 60.4 ± 12.4 years), 86% of whom had an initial diagnosis of early BC and 20% had an ECOG ≥ 2. Median follow-up was 23 months (range 16–28). Metastases were frequently observed in bone (79%) and visceral tissue (65%), with 47% having metastases in > 2 sites. Median number of treatment lines before abemaciclib was 6 (range 1–10). Abemaciclib monotherapy was received by 72% of patients and combination therapy with endocrine therapy by 28% of patients; 54% of patients required dose adjustments, with a median time to first adjustment of 1.8 months. Abemaciclib was discontinued in 86% of patients after a median of 7.7 months (13.2 months for combination therapy and 7.0 months for monotherapy) mainly due to disease progression (69%).

Conclusion

These results suggest that abemaciclib is effective, as monotherapy and in combination, for patients with heavily pretreated mBC, consistent with clinical trial results.
Whose waters, whose nutrients? Knowledge, uncertainty, and controversy over eutrophication in the Mar Menor
Authors: Cabello Violeta , Brugnach Marcela
Published: April 2023

This work explores the role of knowledge claims and uncertainty in the public dispute over the causes and solutions to nonpoint-driven overfertilization of the Mar Menor lagoon (Spain). Drawing on relational uncertainty theory, we combine the analysis of narratives and of uncertainty. Our results show two increasingly polarized narratives that deviate in the causes for nutrient enrichment and the type of solutions seen as effective, all of which relate to contested visions on agricultural sustainability. Several interconnected uncertainties are mobilized to dispute the centrality of agriculture as a driver for eutrophication and to confront strategies that may hamper productivity. Yet, both narratives rest on a logic of dissent that strongly relies on divergent knowledge to provide legitimacy, ultimately reinforcing contestation. Transforming the ongoing polarization dynamics may require different inter- and transdisciplinary approaches that focus on sharing rather than assigning responsibility and that unpack rather than disregard existing uncertainties.
Numerical simulation of nitrogen oxides and carbon monoxide emissions of biodiesel diffusion flame
Authors: Amsal Mohamed , Tran Manh-Vu , Lee Chia Chun , Nurmukan Dastan , Hung Yew Mun , Scribano Gianfranco , Chong Cheng Tung
Published: April 2023

Biodiesel is one of the most promising fossil fuel replacements for automotive engines, furnaces, and turbines due to its sustainability, energy savings, and reduced carbon emissions. While commonly reported in engine studies, nitrogen oxides (NOx) and carbon monoxide (CO) released from combustion of biodiesel have not been studied in laminar diffusion flames. This numerical study examines the concentrations of NOx and CO emissions of the laminar biodiesel diffusion flames at different carbon flow rates and then compares its emissions with those of two liquid hydrocarbon fuel surrogates, n-heptane and iso-octane. A consistent carbon flow rate of 17.2 g/h is applied at the fuel inlet to compare the NOx and CO emissions of the three liquid fuels. The results show that biodiesel diffusion flame produces greater NOx and CO emissions with increasing carbon flow rate. At the same flow rate, n-heptane produces the greatest NO with 2.1% greater than biodiesel and 4.2% greater than iso-octane. The primary pathway for generating NO in biodiesel flame is the prompt pathway, with significant contributions from the thermal and NO2 decomposition pathways. While the NO productions in n-heptane and iso-octane flames are predominantly through the thermal pathway. It is also observed that biodiesel produces the greatest CO emission with 3.2% more than those of n-heptane and iso-octane. The oxidisation reaction of CO, CO + OH = CO2 + H primarily controls the CO mass fraction in the product for all fuels.
Recent Advances of Electrocatalyst and Cell Design for Hydrogen Peroxide Production
Authors: Huang Xiao , Song Min , Zhang Jingjing , Shen Tao , Luo Guanyu , Wang Deli
Published: April 2023

Electrochemical synthesis of H2O2 via a selective two-electron oxygen reduction reaction has emerged as an attractive alternative to the current energy-consuming anthraquinone process. Herein, the progress on electrocatalysts for H2O2 generation, including noble metal, transition metal-based, and carbon-based materials, is summarized. At first, the design strategies employed to obtain electrocatalysts with high electroactivity and high selectivity are highlighted. Then, the critical roles of the geometry of the electrodes and the type of reactor in striking a balance to boost the H2O2 selectivity and reaction rate are systematically discussed. After that, a potential strategy to combine the complementary properties of the catalysts and the reactor for optimal selectivity and overall yield is illustrated. Finally, the remaining challenges and promising opportunities for high-efficient H2O2 electrochemical production are highlighted for future studies.
Advances in Noble Metal-Decorated Metal Oxide Nanomaterials for Chemiresistive Gas Sensors: Overview
Authors: Zhu Li-Yuan , Ou Lang-Xi , Mao Li-Wen , Wu Xue-Yan , Liu Yi-Ping , Lu Hong-Liang
Published: April 2023

Highly sensitive gas sensors with remarkably low detection limits are attractive for diverse practical application fields including real-time environmental monitoring, exhaled breath diagnosis, and food freshness analysis. Among various chemiresistive sensing materials, noble metal-decorated semiconducting metal oxides (SMOs) have currently aroused extensive attention by virtue of the unique electronic and catalytic properties of noble metals. This review highlights the research progress on the designs and applications of different noble metal-decorated SMOs with diverse nanostructures (e.g., nanoparticles, nanowires, nanorods, nanosheets, nanoflowers, and microspheres) for high-performance gas sensors with higher response, faster response/recovery speed, lower operating temperature, and ultra-low detection limits. The key topics include Pt, Pd, Au, other noble metals (e.g., Ag, Ru, and Rh.), and bimetals-decorated SMOs containing ZnO, SnO2, WO3, other SMOs (e.g., In2O3, Fe2O3, and CuO), and heterostructured SMOs. In addition to conventional devices, the innovative applications like photo-assisted room temperature gas sensors and mechanically flexible smart wearable devices are also discussed. Moreover, the relevant mechanisms for the sensing performance improvement caused by noble metal decoration, including the electronic sensitization effect and the chemical sensitization effect, have also been summarized in detail. Finally, major challenges and future perspectives towards noble metal-decorated SMOs-based chemiresistive gas sensors are proposed.
Highly Ordered Thermoplastic Polyurethane/Aramid Nanofiber Conductive Foams Modulated by Kevlar Polyanion for Piezoresistive Sensing and Electromagnetic Interference Shielding
Authors: Qian Kunpeng , Zhou Jianyu , Miao Miao , Wu Hongmin , Thaiboonrod Sineenat , Fang Jianhui , Feng Xin
Published: April 2023

Highly ordered and uniformly porous structure of conductive foams is a vital issue for various functional purposes such as piezoresistive sensing and electromagnetic interference (EMI) shielding. With the aids of Kevlar polyanionic chains, thermoplastic polyurethane (TPU) foams reinforced by aramid nanofibers (ANF) with adjustable pore-size distribution were successfully obtained via a non-solvent-induced phase separation. In this regard, the most outstanding result is the in situ formation of ANF in TPU foams after protonation of Kevlar polyanion during the NIPS process. Furthermore, in situ growth of copper nanoparticles (Cu NPs) on TPU/ANF foams was performed according to the electroless deposition by using the tiny amount of pre-blended Ti3C2Tx MXene as reducing agents. Particularly, the existence of Cu NPs layers significantly promoted the storage modulus in 2,932% increments, and the well-designed TPU/ANF/Ti3C2Tx MXene (PAM-Cu) composite foams showed distinguished compressive cycle stability. Taking virtues of the highly ordered and elastic porous architectures, the PAM-Cu foams were utilized as piezoresistive sensor exhibiting board compressive interval of 0–344.5 kPa (50% strain) with good sensitivity at 0.46 kPa−1. Meanwhile, the PAM-Cu foams displayed remarkable EMI shielding effectiveness at 79.09 dB in X band. This work provides an ideal strategy to fabricate highly ordered TPU foams with outstanding elastic recovery and excellent EMI shielding performance, which can be used as a promising candidate in integration of satisfactory piezoresistive sensor and EMI shielding applications for human–machine interfaces.

Graphical Abstract
Screening of Vitis vinifera cultivars from the Grapevine Germplasm Bank of Aragon for susceptibility to Botryosphaeria dieback fungi
Authors: Langa-Lomba Natalia , González-García Vicente , Martín-Ramos Pablo , Casanova-Gascón José
Published: April 2023

Grapevine trunk diseases (GTDs) are taking the forefront of winegrowers’ concerns, as they cause considerable damage in vineyards not only quantitatively but also qualitatively. Furthermore, in the last three decades, an increasing incidence of the socioeconomic impact of these fungal pathologies has been observed. To date, no effective control strategies or curative treatments are available for these diseases that can replace the effectiveness offered by a series of chemical synthesis fungicides currently prohibited by European legislation. In this scenario, screening for less sensitive cultivars is regarded as a sustainable approach for GTDs management. In the study presented herein, the tolerance/susceptibility of 25 cultivars from the Grapevine Germplasm Bank of Aragon (Movera, Zaragoza, Spain) including commercial, local, or minority germplasm, was tested against two pathogens associated with Botryosphaeria dieback (viz. Neofusicoccum parvum and Diplodia seriata), which were inoculated in a detached cutting assay under open-air conditions. Based on lesion length development after eight months, significant differences were detected among the cultivars in the length of internal (vascular) necroses. In general terms, all cultivars were susceptible to fungal infection, but ‘Macabeo’ and one of the ‘Garnacha Tinta’ ecotypes under study (from Villanueva de Huerva, Zaragoza) would be the least susceptible white and red cultivars, respectively. On the other hand, ‘Monegrina’, ‘Grumel’, and ‘Torcijón’ would be among the least tolerant cultivars to fungal infection.
Building the Leeds Monolingual and Parallel Legal Corpora of Arabic and English Countries’ Constitutions: Methods, Challenges and Solutions
Authors: El-Farahaty Hanem , Khallaf Nouran , Alonayzan Amani
Published: April 2023

Arabic corpora have existed since the last decade of the past century. Although they are constantly increasing, more advanced tools and morpho-syntactically annotated Arabic corpora are still needed for research and teaching. Likewise, parallel and specialised corpora are rare despite the growing need to use them in empirical linguistic investigations of authentic Arabic texts and for language and translation teaching. Therefore, building legal corpora will pave the way for more research in Arabic legal translation, an area which is under-researched worldwide. This paper aims to discuss the building of a collection of specialised parallel and monolingual legal corpora. In particular, it will discuss the building of diachronic corpora, which include all available constitutions of 22 Arabic countries. The aim of building all available versions of these constitutions is two-fold: (1) interdisciplinary corpus-based and socio-cultural investigations and (2) research-led and blended-learning pedagogical approaches to translation teaching and learning. Thus, these corpora are of great value to translation trainers and researchers, law academics and professionals, and governmental, non-governmental and international organisations. The paper will demonstrate the process of building these specialised complex corpora and the challenges encountered throughout this process. Among the challenges faced during the data collection and processing phases are (1) limitations of finding the original constitutions for each Arabic country since some of them date back to 1922; (2) file conversion and the difficulty of choosing one Optical Character Recognition (OCR) tool to rely on for the Arabic language since many lack accuracy, efficiency as well as encoding issues in Arabic.
Statistical analysis of wastewater treatment plant data
Authors: Bourget Gulhan
Published: April 2023

In this paper, we propose to apply the linear mixed effects model (LMEM) for the first time in this discipline to evaluate wastewater treatment plant (WWTP) data. Because the most widely used statistical analyses cannot handle data dependency, as well as fixed and random factors, a new approach was essential. We used the LMEM method to analyze three groups of 19 pharmaceuticals and personal care products (PPCPs) from a California municipal WWTP. We studied the relationships between five treatments, seasons, and three groups of PPCPs. The main contributions of this paper are to investigate three-way and two-way factor interactions and propose that the autoregressive lag 1, AR (1), is a suitable variance-covariance structure for data dependency. While the seasons did not affect the mean concentration levels for the treatments and groups ($$p=0.2540$$

p
=
0.2540

), the mean concentration levels for the seasons differed by groups ($$p<0.0001$$

p
<
0.0001

) and the treatments ($$p=0.0027$$

p
=
0.0027

).
Salt-affected marginal lands: a solution for biochar production
Authors: Wang Yang , Lin Qimei , Liu Zhongzhen , Liu Kesi , Wang Xiang , Shang Jianying
Published: April 2023

The literature has shown that biochar can serve as potential amendment to achieve sustainable agriculture and environment. The accessibility and availability of cheap feedstock are considered as important constraint factors for the widespread application of biochar in agriculture. Marginal lands are widely distributed globally, several times larger than arable land, and hold little value for food production due to poor soil conditions. However, these lands are suitable for growing plants, which can be used as feedstock for biochar production. The salt-affected lands, as one of the main marginal lands, are particularly suitable for cultivating diverse varieties of halophytes that can be pyrolyzed into biochar, bio-gas, and bio-oil. The halophyte-derived biochar is useful to produce a desirable acid soil conditioner due to its high ash and rich bases, and improves soil characteristics under extreme saline conditions. Additionally, syngas and bio-oil hold potential benefits as fuels and industrial raw materials. This study introduces an innovative management technique for marginal lands such as salt-affected land, which can provide all-round benefits in food production, land management, vegetation coverage, carbon sequestration, and climate change mitigation.

Graphical Abstract
Biochar-bacteria partnership based on microbially induced calcite precipitation improves Cd immobilization and soil function
Authors: Xu Min , Ma Jing , Zhang Xiao-Hong , Yang Gang , Long Lu-Lu , Chen Chao , Song Chun , Wu Jun , Gao Peng , Guan Dong-Xing
Published: April 2023

Microbially induced calcite precipitation (MICP) technique utilizes ureolytic bacteria to decompose urea and generate carbonate ions for metal combination. MICP can remediate heavy metal (e.g., Cd) contaminated soils while maintaining or even improving soil functions, but its efficiency in agricultural soil practical application still needs to be enhanced. Here, we constructed a biochar-bacteria (2B) partnership in which biochar provides high nutrition and diverse sorption sites. Using the 2B system, Cd immobilization effectiveness and the underlying mechanism were examined along with the soil properties and soil functions. Results showed that compared to the single biochar and ureolytic bacteria systems, soil Cd mobility was reduced by 23.6% and 45.8% through co-precipitating with CaCO3 as otavite (CdCO3) in the 2B system, whereas soil fertility, bacterial diversity, and richness increased by 11.7–90.2%, 5.4–16.1%, and 6.8–54.7%, respectively. Moreover, the abundances of Proteobacteria and Firmicutes were enhanced in the 2B system. Notably, Sporosarcina and Bacillus (Firmicutes genus) that carry the ureC gene were boosted in the system, further implicating the microbiological mechanism in reducing Cd migration and its bioavailability in soil. Overall, the constructed 2B system was efficient in soil Cd immobilization by strengthening the ureolytic bacteria growth and their nutrient supply in the bacteria-rich soil ecosystem.
Phylotype resolved spatial variation and association patterns of planktonic Thaumarchaeota in eastern Chinese marginal seas
Authors: Liu Jiwen , Huang Fuyan , Liu Jiao , Liu Xiaoyue , Lin Ruiyun , Zhong Xiaosong , Austin Brian , Zhang Xiao-Hua
Published: April 2023

The majority of marine ammonia oxidizers belong to Thaumarchaeota, a phylum of Archaea, which is distributed throughout the water column. Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean, but spatial dynamics of the surface-associated lineages are largely unsolved. This study of 120 seawater samples from the eastern Chinese marginal seas identified contrasting distribution and association patterns among thaumarchaeotal phylotypes across different dimensions. Horizontally, Nitrosopumilus-like and Nitrosopelagicus-like phylotypes dominated the surface water (3 m) of the Yellow Sea (YS) and East China Sea (ECS), respectively, along with increased abundance of total free-living Thaumarchaeota in ECS. Similar compositional changes were observed in the surface microlayer. The spatial heterogeneity of particle-attached Thaumarchaeota was less clear in surface microlayers than in surface waters. Vertically, the Nitrosopelagicus-like phylotype increased in abundance from surface to 90 m in ECS, which led to an increase in the proportion of Thaumarchaeota relative to total prokaryotes. This occurred mainly in the free-living fraction. These results indicate a clear size-fractionated niche partitioning, which is more pronounced at lower depths than in the surface water/surface microlayer. In addition, associations of Thaumarchaeota with other microbial taxa varied between phylotypes and size fractions. Our results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the Nitrosopelagicus-like phylotype is present in shallow shelf waters.
The Internationalisation of Psychology in Brazil: an Analysis of Scientific Outputs, Collaboration Networks and Their Impacts
Authors: Bastos Antônio Virgílio Bittencourt , Gondim Sonia Maria Guedes , Abbad Gardênia da Silva , Mourão Luciana , Pérez-Nebra Amalia Raquel , Coelho Júnior, Francisco Antônio , Carlotto Mary Sandra
Published: April 2023

This article aims
to analyse the process of internationalisation of scientific output in Brazilian psychology and its subfields and compare them with other countries from 2011 to 2020. Two other objectives were taken into consideration: to describe the collaboration networks formed by national psychology researchers and their influence on the impact of scientific output while analysing the relationships in the North–South and South-South axes and to reflect on the advance of the quality of the scientific output over time, considering the indicators of its scientific impact. We used SciVal, based on the Scopus database. The main result is that cooperation on the South-North axis was dominant compared to cooperation on the South-South and South-East axes. The paper also discusses the importance of public funding agencies and the growth of graduate programs in Brazil, enabling the increase in output and the internationalisation of national psychology. The final part addresses the limitations of the Scopus database and some guidelines for the future of the internationalisation of Brazilian psychology.
Controlling Molecular Dye Encapsulation in the Hydrophobic Core of Core–Shell Nanoparticles for In Vivo Imaging
Authors: Umezawa Masakazu , Ueya Yuichi , Ichihashi Kotoe , Dung Doan Thi Kim , Soga Kohei
Published: April 2023

Polymeric nanoparticles with a hydrophobic core are valuable biomedical materials with potential applications in in vivo imaging and drug delivery. These materials are effective at protecting vulnerable molecules, enabling them to serve their functions in hydrophilic physiological environments; however, strategies that allow the chemical composition and molecular weight of polymers to be tuned, forming nanoparticles to control the functional molecules, are lacking. In this article, we review strategies for designing core–shell nanoparticles that enable the effective and stable encapsulation of functional molecules for biomedical applications. IR-1061, which changes its optical properties in response to the microenvironment are useful for in vitro screening of the in vivo stability of polymeric nanoparticles. An in vitro screening test can be performed by dispersing IR-1061-encapsulated polymer nanoparticles in water, saline, buffer solution, aqueous protein solution, etc., and measuring the absorption spectral changes. Through the screening, the effects of the polarity, molecular weight, and the chiral structure of polymers consisting of polymer nanoparticles on their stability have been revealed. Based on the findings presented here, more methodologies for the effective application of various biomolecules and macromolecules with complex high-dimensional structures are expected to be developed.
Global Variations in the Time Delays Between Polar Ionospheric Heating and the Neutral Density Response
Authors: Weimer Daniel R. , Mehta Piyush M. , Licata R. J. , Tobiska W. K.
Published: April 2023

We present results from a study of the time lags between changes in the energy flow into the polar regions and the response of the thermosphere to the heating. Measurements of the neutral density from the Challenging Mini‐satellite Payload (CHAMP) and Gravity Recovery and Climate Experiment (GRACE) missions are used, along with calculations of the total Poynting flux entering the poles. During two major geomagnetic storms in 2003, these data show increased densities are first seen on the dayside edge of the auroral ovals after a surge in the energy input. At lower latitudes, the densities reach their peak values on the dayside earlier than on the night side. A puzzling response seen in the CHAMP measurements during the November 2003 storm was that the density at a fixed location near the “Harang discontinuity” remained at unusually low levels during three sequential orbit passes, while elsewhere the density increased. The entire database of measurements from the CHAMP and GRACE missions were used to derive maps of the density time lags across the globe. The maps show a large gradient between short and long time delays between 60° and 30° geographic latitude. They confirm the findings from the two storm periods, that near the equator, the density on the dayside responds earlier than on the nightside. The time lags are longest near 18–20 hr local time. The time lag maps could be applied to improve the accuracy of empirical thermosphere models, and developers of numerical models may find these results useful for comparisons with their calculations.
Distinct maternal metabolites are associated with obesity and glucose-insulin axis in the first trimester of pregnancy
Authors: Bandres-Meriz Julia , Kunz Christina , Havelund Jesper F. , Færgeman Nils J. , Majali-Martinez Alejandro , Ensenauer Regina , Desoye Gernot
Published: April 2023

Background/Objectives

Obesity in pregnancy associates with changes in the glucose-insulin axis. We hypothesized that these changes affect the maternal metabolome already in the first trimester of human pregnancy and, thus, aimed to identify these metabolites.

Patients/Methods

We performed untargeted metabolomics (HPLC-MS/MS) on maternal serum (n = 181, gestational weeks 4+0–11+6). For further analysis, we included only non-smoking women as assessed by serum cotinine levels (ELISA) (n = 111). In addition to body mass index (BMI) and leptin as measures of obesity and adiposity, we metabolically phenotyped women by their fasting glucose, C-peptide and insulin sensitivity (ISHOMAindex). To identify metabolites (outcome) associated with BMI, leptin, glucose, C-peptide and/or ISHOMA(exposures), we used a combination of univariable and multivariable regression analyses with multiple confounders and machine learning methods (Partial Least Squares Discriminant Analysis, Random Forest and Support Vector Machine). Additional statistical tests confirmed robustness of results. Furthermore, we performed network analyses (MoDentify package) to identify sets of correlating metabolites that are coordinately regulated by the exposures.

Results

We detected 2449 serum features of which 277 were annotated. After stringent analysis, 15 metabolites associated with at least one exposure (BMI, leptin, glucose, C-peptide, ISHOMA). Among these, palmitoleoyl ethanolamine (POEA), an endocannabinoid-like lipid endogenously synthesized from palmitoleic acid, and N-acetyl-L-alanine were consistently associated with C-peptide in all the analyses (95% CI: 0.10–0.34; effect size: 21%;p < 0.001; 95% CI: 0.04–0.10; effect size: 7%;p < 0.001). In network analysis, most features correlating with palmitoleoyl ethanolamide and N-acetyl-L-alanine and associated with C-peptide, were amino acids or dipeptides (n = 9, 35%), followed by lipids (n = 7, 27%).

Conclusions

We conclude that the metabolome of pregnant women with overweight/obesity is already altered early in pregnancy because of associated changes of C-peptide. Changes of palmitoleoyl ethanolamide concentration in pregnant women with obesity-associated hyperinsulinemia may reflect dysfunctional endocannabinoid-like signalling.
High-power continuous-wave optical waveguiding in a silica micro/nanofibre
Authors: Zhang Jianbin , Kang Yi , Guo Xin , Li Yuhang , Liu Keying , Xie Yu , Wu Hao , Cai Dawei , Gong Jue , Shi Zhangxing , Jin Yingying , Wang Pan , Fang Wei , Zhang Lei , Tong Limin
Published: April 2023

As miniature fibre-optic platforms, micro/nanofibres (MNFs) taper-drawn from silica fibres have been widely studied for applications from optical sensing, nonlinear optics to optomechanics and atom optics. While continuous-wave (CW) optical waveguiding is frequently adopted, so far almost all MNFs are operated in low-power region (e.g., <0.1 W). Here, we demonstrate high-power low-loss CW optical waveguiding in MNFs around 1550-nm wavelength. We show that a pristine MNF, even with a diameter down to 410 nm, can waveguide an optical power higher than 10 W, which is about 30 times higher than demonstrated previously. Also, we predict an optical damage threshold of 70 W. In high-power CW waveguiding MNFs, we demonstrate high-speed optomechanical driving of microparticles in air, and second harmonic generation efficiency higher than those pumped by short pulses. Our results may pave a way towards high-power MNF optics, for both scientific research and technological applications.
Scientific rationale for the use of α2A-adrenoceptor agonists in treating neuroinflammatory cognitive disorders
Authors: Arnsten Amy F. T. , Ishizawa Yumiko , Xie Zhongcong
Published: April 2023

Neuroinflammatory disorders preferentially impair the higher cognitive and executive functions of the prefrontal cortex (PFC). This includes such challenging disorders as delirium, perioperative neurocognitive disorder, and the sustained cognitive deficits from “long-COVID” or traumatic brain injury. There are no FDA-approved treatments for these symptoms; thus, understanding their etiology is important for generating therapeutic strategies. The current review describes the molecular rationale for why PFC circuits are especially vulnerable to inflammation, and how α2A-adrenoceptor (α2A-AR) actions throughout the nervous and immune systems can benefit the circuits in PFC needed for higher cognition. The layer III circuits in the dorsolateral PFC (dlPFC) that generate and sustain the mental representations needed for higher cognition have unusual neurotransmission and neuromodulation. They are wholly dependent on NMDAR neurotransmission, with little AMPAR contribution, and thus are especially vulnerable to kynurenic acid inflammatory signaling which blocks NMDAR. Layer III dlPFC spines also have unusual neuromodulation, with cAMP magnification of calcium signaling in spines, which opens nearby potassium channels to rapidly weaken connectivity and reduce neuronal firing. This process must be tightly regulated, e.g. by mGluR3 or α2A-AR on spines, to prevent loss of firing. However, the production of GCPII inflammatory signaling reduces mGluR3 actions and markedly diminishes dlPFC network firing. Both basic and clinical studies show that α2A-AR agonists such as guanfacine can restore dlPFC network firing and cognitive function, through direct actions in the dlPFC, but also by reducing the activity of stress-related circuits, e.g. in the locus coeruleus and amygdala, and by having anti-inflammatory actions in the immune system. This information is particularly timely, as guanfacine is currently the focus of large clinical trials for the treatment of delirium, and in open label studies for the treatment of cognitive deficits from long-COVID.
Viral vectored vaccines: design, development, preventive and therapeutic applications in human diseases
Authors: Wang Shen , Liang Bo , Wang Weiqi , Li Ling , Feng Na , Zhao Yongkun , Wang Tiecheng , Yan Feihu , Yang Songtao , Xia Xianzhu
Published: April 2023

Human diseases, particularly infectious diseases and cancers, pose unprecedented challenges to public health security and the global economy. The development and distribution of novel prophylactic and therapeutic vaccines are the prioritized countermeasures of human disease. Among all vaccine platforms, viral vector vaccines offer distinguished advantages and represent prominent choices for pathogens that have hampered control efforts based on conventional vaccine approaches. Currently, viral vector vaccines remain one of the best strategies for induction of robust humoral and cellular immunity against human diseases. Numerous viruses of different families and origins, including vesicular stomatitis virus, rabies virus, parainfluenza virus, measles virus, Newcastle disease virus, influenza virus, adenovirus and poxvirus, are deemed to be prominent viral vectors that differ in structural characteristics, design strategy, antigen presentation capability, immunogenicity and protective efficacy. This review summarized the overall profile of the design strategies, progress in advance and steps taken to address barriers to the deployment of these viral vector vaccines, simultaneously highlighting their potential for mucosal delivery, therapeutic application in cancer as well as other key aspects concerning the rational application of these viral vector vaccines. Appropriate and accurate technological advances in viral vector vaccines would consolidate their position as a leading approach to accelerate breakthroughs in novel vaccines and facilitate a rapid response to public health emergencies.
Cdc2-like kinases: structure, biological function and therapeutic targets for diseases
Authors: Song Mengqiu , Pang Luping , Zhang Mengmeng , Qu Yingzi , Laster Kyle Vaughn , Dong Zigang
Published: April 2023

The CLKs (Cdc2-like kinases) belong to the dual-specificity protein kinase family and play crucial roles in regulating transcript splicing via the phosphorylation of SR proteins (SRSF1–12), catalyzing spliceosome molecular machinery, and modulating the activities or expression of non-splicing proteins. The dysregulation of these processes is linked with various diseases, including neurodegenerative diseases, Duchenne muscular dystrophy, inflammatory diseases, viral replication, and cancer. Thus, CLKs have been considered as potential therapeutic targets, and significant efforts have been exerted to discover potent CLKs inhibitors. In particular, clinical trials aiming to assess the activities of the small molecules Lorecivivint on knee Osteoarthritis patients, and Cirtuvivint and Silmitasertib in different advanced tumors have been investigated for therapeutic usage. In this review, we comprehensively documented the structure and biological functions of CLKs in various human diseases and summarized the significance of related inhibitors in therapeutics. Our discussion highlights the most recent CLKs research, paving the way for the clinical treatment of various human diseases.
Autophagy deficiency exacerbates iron overload induced reactive oxygen species production and apoptotic cell death in skeletal muscle cells
Authors: Sung Hye Kyoung , Murugathasan Mayoorey , Abdul-Sater Ali A. , Sweeney Gary
Published: April 2023

Iron overload is associated with various pathological changes which contribute to metabolic syndrome, many of which have been proposed to occur via damaging tissue through an excessive amount of reactive oxygen species (ROS) production. In this study, we established a model of iron overload in L6 skeletal muscle cells and observed that iron enhanced cytochrome c release from depolarized mitochondria, assayed by immunofluorescent colocalization of cytochrome c with Tom20 and the use of JC-1, respectively. This subsequently elevated apoptosis, determined via use of a caspase-3/7 activatable fluorescent probe and western blotting for cleaved caspase-3. Using CellROX deep red and mBBr, we observed that iron increased generation of reactive oxygen species (ROS), and that pretreatment with the superoxide dismutase mimetic MnTBAP reduced ROS production and attenuated iron-induced intrinsic apoptosis and cell death. Furthermore, using MitoSox Red we observed that iron enhanced mROS and the mitochondria-targeted anti-oxidant SKQ1 reduced iron-induced ROS generation and cell death. Western blotting for LC3-II and P62 levels as well as immunofluorescent detection of autophagy flux with LC3B and P62 co-localization indicated that iron acutely (2–8 h) activated and later (12–24 h) attenuated autophagic flux. We used autophagy-deficient cell models generated by overexpressing a dominant-negative Atg5 mutant or CRISPR-mediated ATG7 knock out to test the functional significance of autophagy and observed that autophagy-deficiency exacerbated iron-induced ROS production and apoptosis. In conclusion, our study showed that high iron levels promoted ROS production, blunted the self-protective autophagy response and led to cell death in L6 skeletal muscle cells.