bluuebunny/crossref_metadata_2025 huggingface dataset:
Мотивация учения младшего школьника и родительское отношение к ребенку в условиях смешанного обучения
Authors: Вышквыркина Мария А. , Жулина Галина Н. , Лебеденко Ольга А. , Лукьяненко Екатерина С.
Published: December 2022

Введение. Изменившиеся условия существования и обучения, обусловленные карантином и социальными ограничениями в связи с пандемией COVID-19, кардинально трансформировали процессы взаимодействия и коммуникации между людьми. Новизна исследования заключается в получении эмпирических данных, свидетельствующих об изменении характера учебной мотивации и родительского отношения к ребёнку в условиях пандемии и перехода всей системы образования на дистанционный или смешанный формат обучения. Методы. Эмпирическое исследование проводилось с помощью ряда методик: методика «Определение мотивов учения» М. Р. Гинзбурга; опросник «Взаимодействие родитель–ребенок» И. М. Марковской. Исследование проводилось в два этапа в течение 2019–2020 учебного года на базе МАОУ «Школа № 96 Эврика – Развитие им. Нагибина М. В.» г. Ростова-на-Дону. В исследовании приняли участие 107 школьников в возрасте 8–9 лет, а также родители младших школьников в количестве 107 человек (от 30 до 39 лет). Результаты. Полученные результаты выявили наличие некоторых изменений выраженности мотивов учения младших школьников: степень значимости познавательной мотивации младших школьников из семей, склонных к сотрудничеству, во время смешанного обучения стала достоверно ниже, чем при контактном обучении; у младших школьников из семей, склонных к контролю, возросла значимость внешних и игровых мотивов, в то время как выраженность учебных и оценочных мотивов значимо снизилась. Обсуждение результатов. Результаты проведённого исследования соотносятся с выводами других авторов, демонстрирующих снижение школьной мотивации, изменение направленности мотивов и снижение интереса к любимому учебному предмету в условиях дистанционного обучения. Проведенное исследование позволило заключить, что отсутствие непосредственного контакта обучающихся с учителем будет оказывать влияние на повышение значимости внешних и игровых мотивов младших школьников.
Особенности коммуникативного поведения личности подростков с разными уровнями суицидальных рисков при смене реальности
Authors: Лучинкина Анжелика И. , Руденко Екатерина С.
Published: December 2022

Введение. В статье анализируются особенности коммуникативного поведения в виртуальном пространстве подростков с разными уровнями суицидальных рисков. Впервые осуществлено исследование коммуникативного поведения личности подростков с высоким уровнем суицидальных рисков в реальном пространстве при их нахождении в социальных сетях. Большинство исследований доказывает, что пребывание в виртуальной среде оказывает негативное влияние на развитие личности. Учитывая, что подростковый возраст является периодом формирования личности и характеризуется частой сменой настроения, максимализмом, желанием быть в центре внимания референтной группы, подростки зачастую находят себя в виртуальном пространстве, тем самым теряя интерес и навыки коммуникации в реальном, что и приводит к поведению суицидального характера. Методы. Исследование проводилось в реальном и виртуальном пространстве. В констатирующем эксперименте приняли участие 106 подростков в возрасте 14–16 лет. Для выявления групп подростков с повышенными суицидальными рисками в реальном пространстве использовался опросник суицидальных рисков в модификации Т. Н. Разуваевой, суицидальных рисков в виртуальном пространстве – авторская модификация опросника Т. Н. Разуваевой; для изучения коммуникативного поведения подростков применялись методики «Оценка уровня общительности» (тест В. Ф. Ряховского), «Диагностика коммуникативной установки» (В. В. Бойко). Результаты и их обсуждение. Авторы выявили изменения суицидальных рисков у подростков при смене реального пространства на виртуальное. Проанализированы изменения в коммуникативных характеристиках подростков. Установлено, что в виртуальном пространстве у большинства подростков возрастает уровень жестокости, видение окружающей среды приобретает негативную окраску, что увеличивает уровень суицидальных рисков среди подростков.
Structure of Cadmiull}-Substituted Phospholipase A2 from Agkistrodon halys Pallas at 2.8A Resolution
Authors: Lin Zhengjiong , Zhang Hailong , Zhang Yaqun , Song Shiying , Zhou Yuancong
Published: December 2022

The crystal structure of cadmium-substituted basic PLA2 from Agkistrodon halys Pallas (Agkistrodon blomhoffii Brevicaudus, A. h. Pallas) has been determined to 2.8 A with an R factor of 20.1%. Two cadmium-binding sites were found in the enzyme, one is near His48 (S1), the other is near Asp122 (S2). The ligand geometry of the metal ions is described and the inhibition of the catalytic activity by the metal ion is discussed.
Synthesis of Two Designed Hairprin Peptides on a Newly Developed 1,6-Hexanediol Diacrylate (HDODA)-Crosslinked Polystyrene Resin
Authors: Varkey Jaya T. , Awasthi Satish Kumar , Pillai V. N. Rajasekharan
Published: December 2022

Synthesis of two designed hairpin peptides on 1,6-hexanediol diacrylate crosslinked polystyrene support using the standard solid phase methodology is described. Both the peptides are obtained in high yield and purity. The new polymeric system is an ideal support for the synthesis of hairpin peptides, which is a very difficult task by the solid phase method.
Binding of l-Anilino-8-Napthalenesulfonate to Free and Coformycin-Complexed Bovine Adenosine Deaminase
Authors: Britt B. Mark , Cannon Brian , Oakes Jesse A.
Published: December 2022

We have determined the number of binding sites and average binding affinity of the nonpolar probe l-anilino-8- napthalenesulfonate to free bovine adenosine deaminase and to its complex with coformycin, a potent transition state analogue. Our results indicate an increase in average binding affinity accompanied by a reduction in number of sites for the coformycin-complexed enzyme. Interpretation ofthese results in terms of the relative stabilities ofthe two enzyme states and to the catalytic efficiency of enzymes is presented.
The α-Fetoprotein Molecule has One or Two Rigid Domains Depending on the Protein Purification Procedure
Authors: Uversky Vladimir N. , Tomashevski Andrey Yu. , Melnik Tatyana N. , Narizhneva Natalya V. , Shavlovsky Mikhail M. , Vasiliev Vadim B.
Published: December 2022

α-Fetoprotein samples isolated from human cord serum by different methods were compared using circular dichroism, scanning microcalorimetry and fluorescence spectroscopy. Their structural properties and conformational stability were appreciably discriminated. In particular, the protein molecule had either one or two relatively rigid domains which can be co-operatively disrupted by heating or urea treatment. This is due to the difference of the isolation procedures in their capabilities to strip the natural ligands from the protein molecule.
Host Defence Antibacterial Peptides from Skin Secretions of Australian Amphibians. The Relationship Between Structure and Activity
Authors: Bowie John H. , Wegener Kate L. , Chia Brian C.S. , Wabnitz Paul A. , Carver John A. , Tyler Michael J. , Wallace John C.
Published: December 2022

Peptides have been isolated and characterised from the secretions of skin glands of twenty five species of Australian amphibian. Many peptides are host defence agents, showing, for example, neuropeptide and/or antibacterial activity. This review describes the relationship between activity and structure of the antibacterial peptides, particularly the caerin and uperin groups of peptide from the genera Litoria and Uperoleia.
Influence of (;entrally-Administered Peptides on Thirst and Sodium Appetite
Authors: Weisinger Richard S. , Burns Peta
Published: December 2022

This review outlines current knowledge regarding the actions of centrally-administered peptides on the intake of water and/or sodium solutions as well as the possible mechanisms involved. Some peptides facilitate (e.g., angiotensin II), while others decrease or inhibit intake (e.g., somatostatin). Identification of these peptides and/or their receptors in brain regions involved in body fluid and sodium homeostasis, such as the subfomical organ and organum vasculosum of the lamina terminalis, suggests their physiological importance.
Anticariogenic Casein Phosphopeptides
Authors: Reynolds Eric C.
Published: December 2022

Proteins and peptides containing clusters of phosphoseryl residues have been shown to stabilize amorphous calcium phosphate in solution, and have been implicated in the regulation of biomineralisation processes. Casein phosphopeptides (CPP) containing the cluster sequence -Ser(P)-Ser(P)-Ser(P)-Glu-Glu- have been demonstrated to significantly reduce the level of dental caries (tooth decay) in animal and human experiments and also to repair early stages of decay. Through their multiple phosphoseryl residues the peptides bind to forming nanoclusters of amorphous calcium phosphate (ACP) in metastable solution, preventing their growth to the critical size required for nucleation and phase transformation. Conformational and binding studies have shown that all the phosphoseryl residues are important in interaction with ACP. The CPP localise ACP at the tooth surface providing a reservoir of calcium and phosphate ions thereby helping to maintain a state of supersaturation with respect to tooth enamel. The CPP-ACP, unlike fluoride, can be added to sugar-containing foods as well as oral care products for the control of dental caries.
Recent Applications of Instrumental Biosensors for Protein and Peptide Structure-Function Studies
Authors: Nice Edouard , Catimel Bruno , Domagala Teresa , Nerrie Maureen , Weinstock Janet , White Sara , Abud Helen , Heath Joan
Published: December 2022

Since the introduction in the early l 990's of a novel biosensor technology that measures changes in refractive index at or near a sensor surface, the technique has been rapidly embraced by the biological research community to measure a wide range of biomolecular interactions. We will review some recent applications of this technology including preparative ligand fishing and cell-based studies, and discuss the relative merits of different instruments for various applications.
Applications of NMR in Drug Design: Structure-Activity Relationships in Disulfide-Rich Peptides
Authors: Craik David J
Published: December 2022

NMR is a powerful technique for determining structures of biologically active molecules in solution. In recent years.our laboratory has focussed on the structure determination of small disulfide-rich proteins from both plants and animals which are valuable targets in drug design applications. This article will review these structural studies and their implications in drug design.
Synthesis of a New Group of Deltorphin I/II Analogs Modified in the Address Domain with y-Amino Acids, and QSAR Study_ of their δ /µ Opioid Binding
Authors: Heyl Deborah L. , Sanvordekar Medha D. , Dogruyol Guzin , Salamoun Mohamed D. , Rodgers David W. , Renganathan Kutralanathan , Mousigian Carol , Schullery Stephen E.
Published: December 2022

The δ selectivity of the opioid heptapeptides deltorphin I and II has been attributed to the C-terminal "address" domain, the hydrophobic Val5-Val6 residues apparently playing a topographical role. We now report the synthe­sis, opioid binding affinities, and a QSAR study of a series of peptides in which these valine residues were re­ placed with y-amino acids. Results support a conformation stabilization role for this domain, rather than interaction with a receptor binding pocket.
EPO Mimetic Peptide Fused into PAI-1 Augments its EPO Activity
Authors: Zhong Ma , Le-tian Kuai , Chun-lei Wu , Jing Zhang , Qi-feng Qiu , Ai-wu Zhou , Shi-quan Wang , He-yun Zhang , Qian Song , Shu-ning Liao , Yu Han , Jian-ning Liu
Published: December 2022

EPO Mimetic Peptide (EMP) encoding sequence was inserted into the gene of PAI-I between P2'-P3', generating a novel gene of PAI-1/EMP (PMP). It was cloned into pETlld expression vector, and a 43kDa protein was expressed in inclusion bodies with an expression level over 30%. The PMP protein was purified by DEAE ion-exchange chromatography to one band in SDS-PAGE (Silver Stained). The EPO activity of PMP was determined by Reticulocyte Counting Assay to be S000IU/mg, 2500 folds that of EMP (as reported).
Effect of Copper Ion on Conformations of Short Alanine-Based Peptides with Single TRP/HIS Stacking Interactions
Authors: Sugimoto Naoki , Zou Jin
Published: December 2022

cu2+ has an apparent promoting effect on deaggregating β-sheet conformation, conformational
transitions from β-sheet to α-helix, and enhancing α-helix conformation of short alanine-based peptides with single Trp/His (W/H) stacking interactions in different geometrical spacings and positions, respectively.
Crystallization and A Preliminary Crystallographic Analysis of the Catalytic Domain of Chitinase Al from Bacillus Circulans WL-12
Authors: Katouda Haruhiko , Hashimoto Masayuki , Watanabe Takeshi , Matsumoto Takuo , Nonaka Takamasa , Mitsui Yukio
Published: December 2022

The catalytic domain of chitinase Al from Bacillus circulans WL-12 was crystallized by vapor­ diffusion technique. The crystals belong to the triclinic space group Pl with cell dimensions a = 43.96A, b = 48.62A, c = 54.59A, a =108.90°, f3 = 95.06°, and y= 115.77°, and contain one molecule in the asymmetric unit. High-quality diffraction data were collected at 20°C with radiation from a synchrotron source up to 1.5A resolution.
Crystallization and Preliminary X-ray Crystallographic Studies of Ribonuclease LE from Lycopersicon esculentum
Authors: Nakamura Kazuo T. , Tanaka Nobutada , Arai Jun , lnokuchi Norio , Koyama Takashi , Ohgi Kazuko , Irie Masachika
Published: December 2022

The ribonuclease LE, a plant ribonuclease from lycopersicon esculentum, has been crystallized by the hanging-drop vapour diffusion method using polyethylene glycol 1,540 as the precipitating agent. The
crystals belong to an orthorhombic space group P2,2,2, with cell dimensions of a = 74.10 A, b = 78.72 A, and c
= 33.00 A. There is one molecule per asymmetric unit. The crystals diffract to at least 2.0 A resolution and
are suitable for X-ray structure analysis at high resolution.
The cysteine-to-histidine substitution in domain of metallothionein enhances its zinc binding ability
Authors: Ru Binggen , Zhou Yanjiao , Zhang Ning , Li Lingyuan
Published: December 2022

A two-step PCR method was taken to replace the cysteines at position 19, 29 in p domain of Metallothionein with histidines. Then the His19·29-mutant fragment was cloned into the vector pGEX-4T-l and expressed as a fusion protein of glutathione-S-transferase. After cleaved with thrombin and purified, the p domain with the substitution of Cys-to-His was obtained and identified by amino acid composition and molecular weight. The His19·29 mutant can bind with the same amount of zinc or cadmium ions as the p domain but exhibits stronger zinc binding ability and weaker cadmium binding ability.
Expression, purification, crystallization and preliminary x-ray analysis of the augmenter of liver regeneration
Authors: Wu Chia-Kuei , Dailey Tamara A. , Dailey Harry A. , Francavilla Antonio , Starzl Thomas E. , Wang Bi-Cheng , Rose John P.
Published: December 2022

A novel grow.th factor termed augmenter of liver regeneration (ALR) coptaining an N-terrninal histidine tag has been expressed, purified and crystallized. The crystals diffract to beyond 2 A, belong to space group 141 with a= 99.8A and c = 113.4A, and have four molecules per asymmetric unit. Cadmium proved essential to the crystallization of the protein and the positions of four cadmium atoms have been determined by Bijvoet difference Patterson analysis. A possible role for cadmium stabilization of the his-tagged protein lattice is discussed.
Wolff rearrangement of Na-protected a-aminodiazoketones in presence of substituted phenol as a method for the synthesis of 2,4,5- trichlorophenyl esters of Fmoc-/ Boe-/ Z-P-homoamino acids
Authors: Babu Vommina V. Suresh , Gopi Hosahuciya N. , Ananda Kuppanna
Published: December 2022

Silver acetate catalyzed decomposition of the Na-protected a­ aminodiazoketones in the presence of 'substituted phenol' and triethylamine at -
1s0c lead to 2,4,S-trichlorophenyl esters of Fmoc-/Boc-/Z-J3-homoamino acids in
good yield and purity. Thus the homologation of a-amino acids to 13-homoamino acids with concomitant active esters formation has been achieved by this method.
Cytotoxicity and membrane perturbation associated with the myristylated n-terminal portion of hiv-1 gag protein
Authors: Macreadie I. G. , Curtain C. C.
Published: December 2022

The Gag and Nef proteins of human immunodeficiency type l (HIV-1) have conserved N-terminal sequences that appear essential for HIV-1 function. In view of the structural similarities a Gag peptide corresponding to myrGag2_22, was subjected to cytotoxicity tests employing recently developed cell assays. The Gag peptide displays a similar activity to Nef. The role of such sequences may be important in viral membrane association, viral biogenesis and transition of these proteins through the cellular membrane.
The specificity of enteropeptidase relative to the chimeric proteins and trypsinogen processing
Authors: Shibanova Elena , Alexandrov Sergey , Miroshnikov Ariatolii
Published: December 2022

The limited proteolysis by enteropeptidase of the recombinant chimeric proteins incorporating the linker (Asp)4- Lys was studied. The resistant to the hydrolysis chimeric proteins was shown to be the competitive inhibitors in re­ lation to the effectively splitting chimeric proteins and to the low molecular intermediates of the linker, but not of the trypsinogen processing. The linker was shown to be defined the binding of the protein substrates by enzyme only, rather than the efficiency, determined by the C-terminal component of enzyme specificity.
Human nucleotide excision repair protein xpa: summary of exafs studies on the zn (ii), co (ii) and cd
(ii) associated minimal dna-binding domain
Authors: Kennedy Michael A. , Buchko Garry W. , Hess Nancy J.
Published: December 2022

The zinc in the metal-binding core of the DNA-binding domain of the nucleotide excision repair protein XPA (M98-F219) can be replaced with cadmium (II) and cobalt (II). Here, we summarize extended X­ ray fine structure spectra collected on each protein in the lyophilized state and in 15% frozen aqueous glycerol solution. Under both conditions the Zn2•, Cd2+,and Co2• are tetrahedrally coordinated to the sulfur atom of four cysteine residues with the Zn-S and Co-S bond lengths nearly identical, at 2.34 A, and the Cd-S bond length at 2.54A.
Equilibrium unfolding of yeast triosephosphate isomerase: a monomeric intermediate in guanidine-hci and two-state behavior in urea
Authors: Vazquez-Contreras Edgar , Zubillaga Rafael A. , Mendoza-Hernandez Guillermo , Costast Miguel , Fernandez-Velascot D. Alejandro
Published: December 2022

This is the first experimental evidence of an equilibrium intermediate in the unfolding of triosephosphate isomerase (TIM). The reversible unfolding of S. cerevisiae TIM induced by both guanidine­ HCl (Gdn-HCl) and urea, are apparently monophasic when followed by spectroscopic techniques. Kinetic analysis and ANS binding data confirm a two-state transition in urea. nevertheless, in Gdn-HCl they indicate an intermediate. Hydrodynamic properties of the intermediate are consistent with a compact monomer.
Occurrence of an essential amino acid residues in the lytic effect of escherichia coli a -haemolysin
Authors: Bakas Laura
Published: December 2022

α-Hemolysin (HlyA), an extracellular protein toxin produced by some pathogenic strains of Escherichia coli , is known to disrupt eukaryotic cell membranes. In an attempt to correlate the structure of this toxin with its function, we studied the role of the histidine residues on the toxin activity by their chemical modification with the reagent diethyl pyrocarbonate (DEPC). The results of this study suggest the presence of an essential histidine residue at the Ca2+-binding motif and it.s role in the lytic action of E.coli HlyA.
Analysis of the membrane interactive potential of the escherichia coli pbp6b c-terminus
Authors: Phoenix David A. , Wallace James
Published: December 2022

Escherichia coli low molecular weight penicillin binding proteins include PBP4,5,6 and recently PBP6b. PBP5 and PBP6 have been shown to possess C-terminal amphiphilic helical anchors. PBP4 has been shown to have the potential to interact at the membrane interface but the protein appears to bind via a different mechanism to PBP5 and PBP6. It has been suggested that since PBP6b has a high homology with PBP6 it also binds via an amphiphilic alpha helix. Here helical wheel, hydrophobic moment and DWIH analysis are used to show that the C-terminus of PBP6b does not resemble the anchor regions of the PBP5 and 6 but may stabilize membrane binding using a mechanism similar to that of PBP4.
Protein photodegradation in the fluorescence unfolding kinetics study of bromelain
Authors: Arroyo-Reyna Alfonso , Tello-Solis Salvador R.
Published: December 2022

We have detected protein photodegradation in the fluorescence unfolding kinetics experiment of stem bromelain, a cysteine proteinase from the papain family. Thermally induced unfolding has been followed by near and far circular dichroism and tryptophan fluorescence emission. It was found that the fluorescence kinetics involves two phases. This biphasic nature results from ultraviolet induced photolysis oftryptophan.
Conformational changes of human neuronal tau during thermal and guanidine-hcl denatura tion
Authors: Be Rong-Qiao , Luo Jian-Ying , Liu Yang , Bua Qian
Published: December 2022

Unfolding of human neuronal tau was studied during incubation with guanidine hydrochloride (GuBCI) and under thermal denaturation conditions. The intensity of the intrinsic fluorescence at 305 nm increased markedly in GuBCI solutions. Fluorescence quenching by potassium iodide showed that Tyr residues approached to the interior of the molecule during GuBCI denaturation. The difference ultraviolet absorbance at 215 nm showed a marked increase above 45°C. Light scattering of tau solution
· intensity decreased when temperature increased. Kinetics of the increase in the absorbance was a biphasic procedure. The first order rate of the fast phase was 1.84 x 10-1s-1 and that of the slow phase was
2.68 x 10-2s-1• The difference absorbance of tau was reversible. Kinetics of a decrease in the difference absorbance was a fast procedure during tau refolding. The first order rate of the fast phase was much greater than that of the unfolding procedure. This suggests that tau is not completely at a random conformation at room temperature.
Synthetic glycopeptide and phosphopeptide models of an antibacterial fragment of chromogranin A
Authors: Otvos, Jr. Laszlo , Cudic Mare , Bulet Philippe
Published: December 2022

In order to fully characterize the activity spectrum and additional biochemical properties of native antimicrobial peptides corresponding to chromogranin A, we synthesized glycosylated, phosphorylated and double modified versions of the reportedly antibacterial 173-194 fragment. While no conformational change was detected, the modifications stabilized the peptide from proteolytic cleavage. All peptides remained inactive against a number of Gram-positive and Gram-negative bacterial strains in the experimental conditions use .
3D-structure of human seminal plasma prostatic inhibin by nonparametric regression
Authors: Jyothi S. , Joshi Rajani R.
Published: December 2022

Human Seminal Plasma Prostatic Inhibin (HSPI) is a biologically important protein of 94 amino acids isolated from the human prostate glands. No information is yet available on its 3d-structure. Here, we use a nonparametric regression approach to determine the 3d-structure of HSPI. This approach has been validated earlier and is found to be better in accuracy and efficiency than other computational approaches. The structure predicted by us agrees with the properties ofHSPI deciphered experimentally.
Prediction of some spatial structures of proinsulin by hydropathic mass
Authors: HE Rong-Qiao , Liu Yang , Liu Ying
Published: December 2022

Hydropathic mass (HM) has been used to display the characterization of hydrophilicity and hydrophobicity of proinsulin. The a-helix of the B chain (residues 9-19), which is located at the inner part of the crystalline insulin, possesses a strong positive HM. The proteolytic sites of proinsulin for insulin maturation are located in the most negative HM regions. This suggests that they are exposed to the exterior of the molecule, which contributes to the digestion by proteases during insulin maturing.
Conformation of the α-aminoacrylate intermediate of o-acetylserine sulfhydrylase from salmonella typhimurium as inferred by 31p nmr spectroscopy
Authors: Tai Chia-Hui , Cook Paul F. , Schnackerz Klaus D.
Published: December 2022

31P NMR chemical shifts of the internal aldimine and the cx.-aminoacrylate intermediate of 0- acetylserine sulfhydrylase were determined and found to be 5.13 and 3.95 ppm, respectively. In addition, the line width decreases upon addition of OAS, suggesting a less restricted rotation around the CS'-0 bond of pyridoxal 5' -phosphate in the cx.-aminoacrylate intermediate The upfield shift of the cx.-aminoacrylate is similar to the behavior of the L-serine external Schiff base and may be interpreted as a loosening of the active site.
Simulation of the highly stable protein: bovine γb-crystallin at room and high temperature
Authors: Goodfellow Julia M. , Purkiss Andrew G. , Slingsby Christine
Published: December 2022

The behavior of the eye-lens protein bovine γB-crystallin is investigated at both room temperature and at an elevated temperature (at which unfolding would be expected to occur) using molecular dynamics. Even in the high temperature simulation, there is little loss ·of secondary structure. Although only small changes in the tertiary contacts occur, even at high temperature, fluctuations in conformation are much more pronounced at 500K than 300K. These results agree with the experimental observations on the remarkable stability of the eye lens proteins and also indicate where the initial events in unfolding are occurring.
Molecular modeling of arcelin-5c from bean seeds and determination of its solution state
Authors: Cordeiro Artur T. , Gerhardt Isabel R. , de Oliveira-Neto Osmundo B. , Bloch Jr. Carlos , de Sa Maria Fatima Grossi
Published: December 2022

Phaseo/us arcelin proteins are known to be involved in resistance mechanisms to bruchid pests in plants. The mechanism of action against the larvae is still unclear, but it seems to be related to the oligomeric state and stability of the protein in solution. The solution state of arcelin-5 variants was analyzed by size exclusion chromatography and a molecular model of arcelin-5c was built and compared with the structure of arcelin-1 and phytohemagglutin-L.
Expression and purification of human metallothionein-3 beta domain in e.coli
Authors: Wutt Daqing , Shen Jiong , Ru Binggen
Published: December 2022

J3-domain of metallothionein-3/growth inhibitory factor(MT-3/GIF) is crucial to the neuronal growth inhibitory activity of MT-3. The MT-3 J3 domain was efficiently expressed as the soluble fusion form with GST protein at a high level which accounted for over 30% total cellular proteins. After the thrombin cleavage and purification, the bioactive recombinant MT-3 J3 domain was recovered with the yield of 6 mg/L of medium.
Crystallization and preliminary crystallographic studies of pekin duck oxyhemoglobin
Authors: Wang Huan-Chen , Zha Zheng , Liang Yu-He , Hua Zi-Qian , Lu Guang-Ying
Published: December 2022

Pekin duck oxy hemoglobin has been purified and crystallized using PEG 6000 as precipitant. The crystal is of space group P41212 with lattice constants a= b = 81.23, c = 216.3 A. There is one tetramer molecule per asymmetric unit. The crystal diffracts up to 2,8 A resolution and is suitable for X-ray crystallographic studies.
Structural studies of a human malic enzyme
Authors: Tong Liang , Yang Zhiru
Published: December 2022

Structural studies of the human mitochondrial NAD(Pt-dependent malic enzyme (m-NAD-ME) establish that malic enzymes belong to a new class of oxidative decarboxylases: An open and a closed form of the enzyme have been observed from the crystallographic analysis, with the closed form also revealing the binding modes of the divalent cation and the transition-state analog inhibitor oxalate. The structures show that E255, D256 and D279 are the ligands of the cation, and suggest that Y112 and K183 may be the catalytic residues of the enzyme.
Chemical mechanism of malic enzyme as determined by isotope effects and alternate substrates
Authors: Cleland W. W.
Published: December 2022

Isotope effect studies have shown that malic enzyme from avian liver or Ascaris suum has a stepwise mechanism with NAD(P) as substrate, but a concerted one with nucleotide substrates with higher redox potentials. It has been possible to calculate intrinsic deuterium and 13C isotope effects and commitments. The enzyme is very
specific, with only erythro-fluoromalate, D- and mesotartrate and L-aspartate with an unprotonated amino group as slow alternate substrates.
Chemical mechanism of 6-phosphogluconate dehydrogenase via kinetic studies and site­ directed mutagenesis
Authors: Cook Paul F. , Zhang Lei
Published: December 2022

6-Phosphogluconate dehydrogenase catal)jzes the metal ion-independent oxidative decarboxylation of 6- phosphogluconate (6PG). The enzyme catalyzes a reaction in which 6PG is first oxidized at the 3-position, then decarboxylated to the 1,2-enediol of Ru5P , and then tautomerized to give the Ru5P ketone product. In the reaction, Kl83 acts as a general base, general acid, shuttling a proton between the 3-hydroxyl and itself, and E190 acts as a general acid to protonate C1 of the enediol intermediate in the tautomerization step.
6-Phosphogluconate dehydrogenase: Structural symmetry and functional asymmetry
Authors: Dallocchio Franco , Rippa Mario , Hanau Stefania , Cervellati Carlo
Published: December 2022

6-Phosphogluconate dehydrogenase catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate through an oxidation, a decarboxylation and a tautomerization. The two subunits in the crystals of the dimeric sheep liver enzyme have the same conformation, as the apoenzyme and with the substrate or coenzyme. An hypothesis is now advanced that in solution, during catalysis, the two subunits have a different alternating role and thus a functional asymmetry.
Synthesis of peptide with multiple cysteic acids
Authors: Arendt Anatol , McDowell J. Hugh , Smith W. Clay , Hargrave Paul A.
Published: December 2022

We have synthesized and purified a 19-amino acid peptide whose sequence is derived from the carboxyl-terminal region of bovine rhodopsin, in which the seven serines and threonines that would normally become phosphorylated have been substituted with cysteic acid. The peptide was synthesized by the Fmoc (DCC/HOBl)' procedure using Fmoc-(S-4-methoxytrityl) Cys {Fmoc-Cys(Mmt)-OH}. The Mmt group was removed and the resin-bound peptide was oxidized with performic acid, which also removed other blocking grou_psand cleaved the peptide from the resin.
Physicochemical properties and behavior in
solution of three cellulases from haliotis fulgens
Authors: Rodrfguez-Romero Adela , Hernandez-Santoyo Alejandra , Rojo-Domfnguez Arturo , Garcfa-Hernandez Enrique
Published: December 2022

Three proteins that showed activity over cellulosic substrates were isolated from the hepatopancreas of the blue abalone Haliotis fulgens. The purified cellulases are acidic with molecular weights between 17 900 and 30 300. Activity experiments indicated that they are endo-, exo- and l3-glucanases. These proteins form large aggregates as demonstrated by means of DLS experiments, which can be dissociated in the presence of polyethylene glycol and mannitol. The circular dichroism spectra in the far UV indicated that the enzymes belong to the 13-13 family.
Stability of a black eyed pea trypsin/chymotrypsin inhibitor (BTCI)
Authors: de Freitas Sonia Maria , Silva Luciano Paulino , Leite Jose Roberto S. A. , Bloch Jr. Carlos
Published: December 2022

The stability of BTCI has been investigated as function of pH and temperature, following its inhibitory activity against trypsin. The isolated inhibitor of 9,084 Oa is stable over pH 3 to 12 at 25°C. BTCI showed high thermal stability ranging from 25 to 95°C at pH 3 and 7. However, the protein lost about 20% of its inhibitory activity over 75°C at pH 8.2. The results indicated that BTCI is extremely stable to heat and pH as typical of Bowman-Birk inhibitors.
Understanding the contribution of individual tryptophan residues to intrinsic lysozyme fluorescence
Authors: Uversky Vladimir N. , Kuznetsova Irina M. , Biktashev Alexander G. , Malova Lubov' N. , Bushmarina Natalia A. , Turoverov Konstantin K.
Published: December 2022

Packing density and other properties of the microenvironment of tryptophan residues in hen egg-white lysozyme macromolecule have been studied on the basis of the known 3D structure of this protein. Results presented here suggest that the efficiency of action of the group which, in principal, can affect tryptophan fluor scence depends not only on the distance between this group and the indole ring, but to a great extent upon the location of this group relative to the indole ring.
effect of return on assets, debt to equity ratio, and corporate social responsibility policies on company values in PT Semen Baturaja (Persero) Tbk.
Authors: Royhaan Ahmad , Isnurhadi Isnurhadi , Widiyanti Marlina , Adam Mohamad
Published: December 2022

This study aimed to know the effect of return on assets, debt to equity ratio, and corporate social responsibility policies on score company at PT Semen Baturaja (Persero), Tbk. The population and sample in the study is a company engaged in the cement sector, namely PT Semen Baturaja (Persero), Tbk, which uses report finance during periods 1-4 of 2012-2021. Based on the analysis of the results, multiple linear regression that has been conducted shows that return on assets take an adverse effect and has no significance to score Company, equity debt has an effect positive and significant to score Company, corporate social responsibility has an effect positive and significant to score Company. For companies in the cement industry, especially Semen Baturaja, it is necessary to develop ROA, DER, and CSR as tools to increase company value so that they can continue to compete and improve. For regulators, the results of this study provide an overview of ROA, DER, and CSR towards score companies. Therefore, regulatory agencies must set standards for measuring and disclosing existing corporate values.
Diyabet Merkezden Takipli Tip 2 Diabetes Mellitus Hastalarında Hipoglisemi Sıklığı ve Hipoglisemi Korkusu
Authors: ÇALIŞKAN Sema Gizem , HACIAĞAOĞLU Nazlı , TUZUN Sabah , ÖNER Can , ŞİMŞEK Engin Ersin , CETİN Huseyin
Published: December 2022

Giriş: Bu çalışma aile hekimliği yönelimli diyabet merkezi (AHYDM) ile hastane yönelimli diyabet merkezi (HYDM)’den takipli tip 2 diabetes mellitus (DM) hastalarında hipoglisemi sıklığı ve ilişkili faktörlerin karşılaştırılmasını amaçlamaktadır. Yöntem: Bu çalışmaya 1 Aralık 2017– 31 Aralık 2019 tarihleri arasında aynı hastaneye bağlı olan iki diyabet merkezine başvuran Tip 2DM hastaları dahil edilmiştir. Çalışmada hastane içerisinde yer alan ve İç Hastalıkları Kliniği tarafından yürütülen DM merkezi HYDM olarak kabul edilirken, hastane kampüsü dışında bulunan, Aile Hekimliği Kliniği tarafından yürütülen DM merkezi ise AHYDM olarak kabul edilmiştir. Her iki merkezdeki açlık plazma glukozu düzeyi en az iki defa 70 mg/dL’nin altında saptanan hastalar vaka grubuna dahil edilmiştir. Ardından, her bir merkezde vaka grubundaki hastalara yaş ve cinsiyet açısından eşleştirilmiş, hipoglisemi atağı bulunmayan Tip 2 DM hastaları kontrol grubu olarak kabul edilmiştir. Ayrıca, Hipoglisemi Korku Ölçeği (HKÖ) iki merkezdeki vaka grubuna da uygulanmıştır. Bulgular: Çalışmaya dahil edilen 240 hastanın 150 (%62,5)’si kadın idi. HYDM’deki tüm hastaların 53(%1,2)’ünde hipoglisemi saptanmışken, AHYDM’den katılan tüm hastaların 27(%0,31)’sinde hipoglisemi tespit edilmiştir. Her iki merkezdeki vaka grubunda DM süresi, insülin kullanım sıklığı ve toplam insülin dozu kontrol grubundan yüksekti (sırasıyla, HYDM için p=0,049, p<0,001, p=0,001; AHYDM için p=0,014, p<0,001, p=0,010). Ayrıca AHYDM'deki vaka grubunda hipoglisemi eğitimi alma sıklığı ve hipoglisemi eğitiminin yeterli olduğu algısı HYDM'ye göre daha yüksekti (sırasıyla p=0,029 ve p=0,046). İki merkezde de HKÖ’de fark bulunmamıştır (p>0,05). Sonuç: DM hastalarında, özellikle insülin kullanan ve T2DM süresi uzun olan hastalarda hipoglisemi açısından dikkatli değerlendirilmesi gereklidir. Ayrıca hastaların aile hekimleri tarafından hipoglisemi ile ilgili eğitilmesi hipoglisemi görülme sıklığını azaltabilir.
Determination of Dyadic adjustment, life satisfaction, depression, and female sexual dysfunction in married women during the COVID-19 pandemic
Authors: ALAN DİKMEN Hacer
Published: December 2022

Aim: The COVID-19 (Coronavirus disease- 2019) pandemic negatively affects the sexual functions of women as well as their psychological health. The purpose of the study was to evaluate dyadic adjustment, life satisfaction, depression, and female sexual dysfunction (FSD) in married Turkish women during the COVID-19 pandemic. Methods: This is a descriptive study. Data were collected online between May 29th, 2020 and June 22th, 2020 with the Revised Dyadic Adjustment Scale (RDAS), Satisfaction with Life Scale (SWLS), Center for Epidemiologic Studies Depression Scale (CES-D), Female Sexual Function Index (FSFI), and questionnaire form from 210 married women. Results: The average RDAS and SWLS scores of the women were 50.55 ± 9.68 and 16.51 ± 4.96, respectively. While 63.3% of the women had 'depression', there was FSD in 88.1%. In the study, there was a statistically significant difference between FSD presence and RDAS (p < 0.001) and SWLS (p < 0.001). There was no statistically significant difference between the presence of FSD and depression (p = 0.078). Conclusion: This study will enable health professionals (especially female health nurses, psychiatric nurses, and sexual and family therapists) to evaluate and be aware of the presence of FSD, and dyadic adjustment, life satisfaction, and depression levels in married women during the COVID-19 pandemic.
Gebelerde Tutarlılık Duygusu, Algılanan Stres ve Doğum Korkusu
Authors: AKIN Bihter , ÇANKAYA Seyhan
Published: December 2022

Giriş: Çalışma gebelerde tutarlılık duygusu, algılanan stres ve doğum korkusunu belirlemek amacıyla gerçekleştirilmiştir. Yöntem: Tanımlayıcı tipte olan bu çalışma 01 Ocak-31 Mart 2022 tarihleri arasında gerçekleştirilmiştir. Çalışmaya araştırma kriterlerine uyan İç Anadolu Bölgesi’nde yaşayan toplam 218 gebe dahil edilmiştir. Veriler 'Kişisel Bilgi Formu', 'Revize Edilmiş Duygusal Tutarlılık Ölçeği (REDTÖ)', 'Algılanan Stres Ölçeği (ASÖ)' ve 'Wijma Doğum Beklentisi/Deneyimi Ölçeği (W-DEQ) A versiyonu (WİJMA A)' kullanılarak çevrim içi toplanmıştır. Verilerin analizinde bağımsız gruplarda t testi kullanılmıştır. Bulgular: Gebelerin REDTÖ, ASÖ ve WİJMA A ölçek toplam puan ortalamaları sırasıyla 43,41±9,23, 22,73±5,35 ve 76,46±17,52 olarak bulunmuştur. Gebelerin tutarlılık ve doğum korku düzeylerinin yüksek, stres düzeylerinin de orta olduğu görülmektedir. Sosyal güvencesi olmayan ve isteyerek gebe kalmayanların stres ve doğum korku düzeyinin diğer gebelere oranla daha yüksek olduğu bulunmuştur (p<0,05). Araştırmadan elde edilen bir diğer önemli sonuç ise daha önce sezeryan ile doğum yapmış kadınların normal doğum yapanlara göre daha fazla doğum korkusu yaşadığının belirlenmesidir (p<0,05). Sonuç ve Öneriler: Araştırmadan elde edilen veriler önceki doğum şekli, gebeliği isteme ve sosyal güvence durumunun doğum korku düzeyi üzerinde olumsuz etkisi olduğunu göstermektedir. Doğum korkusunun uzun ve kısa vadede anne-çocuk sağlığı üzerinde etkileri düşünüldüğünde özellikle risk grubundaki kadınların doğum korku düzeyinin belirlenmesi ve bu düzeyin azaltılmasına yönelik stratejilerin geliştirilmesine ihtiyaç duyulmaktadır.
Inter- and Intra-Rater Reliability of the Measure of Patient-Centered Communication
Authors: GÜNVAR Tolga , GÜREL Yunus , GÜLDAL Dilek , BAŞAK Okay
Published: December 2022

Introduction: The patient-centered clinical method allows the biopsychosocial model to be implemented in clinical practice. It is critical to look at the use of the patient-centered clinical method, which has been shown to benefit both patients and clinicians, particularly in primary care. The Measure of Patient-Centered Communication (MPPC) is a theory-based instrument for assessment of patient-physician interaction. The aim of this research is to investigate the inter- and intra-rater reliability of the MPCC tool in Turkish. Methods: Audiovisual recordings of 60 patient-physician consultations of 30 family physicians were evaluated. Three researchers independently assessed and scored these interviews with MPCC by following the instructions in the manual of the tool. Evaluators reassessed the randomly selected 20 consultations 15 days later to determine the intra-rater reliability. For each component score and the overall score, ICC estimates, and their 95% confidence intervals were calculated based on a mean-rating (k=3), consistency, 2-way mixed-effects model. Results: The ICCs for overall score and component one were 0.810 and 0.820, respectively, for all 60 consultations, demonstrating strong inter-rater reliability. Components two and three had ICCs of 0.646, indicating strong reliability, and 0.537, indicating moderate reliability. All researchers' intra-rater correlation scores for all score groups ranged between 0.989 and 0.698, indicating good to excellent reliability. Conclusions: MPCC tool is reliable in its current form as it is translated into another language and conducted in another sociocultural environment.
Tıp Öğrencileri Arasında COVID-19 Aşı Tereddütü ve İlişkili Faktörler, Bolu Örneği
Authors: AKBAŞ GÜNEŞ Nurcan , GÜNEŞ Akif
Published: December 2022

Giriş: Tıp öğrencileri, COVID-19 hastaları ile temas eden ön saflardaki sağlık hizmeti sağlayıcıları arasındadır. Bu grupta yüksek COVID-19 aşılama oranlarına ulaşmak gerekli ve önemlidir. Bu çalışmanın amacı, Türkiye’de tıp fakültesi öğrencilerinin COVID-19 aşısına karşı aşı tereddütlerini ve ilişkili faktörleri değerlendirmektir. Elde edilen bilgiler potansiyel endişelerin belirlenmesine yardımcı olacaktır. Yöntem: Çalışmamız kesitsel tipte dizayn edilmiştir. Bolu Abant İzzet Baysal Üniversitesi Tıp Fakültesinde eğitim gören 225 öğrenci çalışmaya dahil edilmiştir. Veri toplama aracı olarak ilgili literatür taranarak değerlendirme formu oluşturulmuştur. Bulgular: Katılımcıların %53,3’ünün (n=120) COVID-19 aşısı ile ilgili tereddütü yoktu, % 46,7’si (n=105) ise çeşitli nedenlerle aşı tereddütü yaşıyordu. %22,7 (n=51) ile en yüksek oranda 'uzun dönem etkileri konusunda tereddütleri olduğu' belirtildi. COVID-19 enfeksiyonu geçiren katılımcılar halk sağlığı uzmanları ve aile hekimlerinden aldıkları aşı hakkındaki bilgilere güvenmiyordu (p=0,237). Sonuç: Türkiye’deki tıp fakültesi öğrencilerinin COVID-19 aşılarının uzun dönem etkileri konusunda tereddütleri olduğu görülmektedir. Hekim ve hekim adaylarının bu tereddütleri giderilmelidir. Çalışmamız sonucunda bir sonraki yıl grip aşısı yaptırmayı düşünenlerin oranının yaklaşık 10 kat arttığı görülmektedir. Pandemi öğrencilerin aşı olma isteğini artırmış gibi görünmektedir.
Çocukluk Çağı Aşı Tereddüdü ile Karşılaşma Sıklığı, Nedenleri ve Çözüm Önerileri: Samsun İli Aile Sağlığı Merkezlerindeki Sağlık Çalışanlarıyla Kesitsel Bir Çalışma
Authors: ALTUNTAŞ Mehtap , ŞAHİN Mustafa Kürşat
Published: December 2022

Amaç: Bu çalışmada, Samsun ilindeki aile sağlığı merkezlerinde görev alan sağlık çalışanlarının çocukluk çağı aşı tereddüdü ile karşılaşma sıklığı, nedenleri ve çözüm önerilerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Kesitsel tipte bir çalışmadır. Samsun’da Mayıs-Eylül 2021’de online bir anket ile yürütülmüştür. Çalışmaya 165 aile hekimi, 131 aile sağlığı çalışanı katılmıştır. Bu çalışma kapsamında, aile sağlığı biriminde çalışan hekim ve diğer sağlık personeli tek tek telefonla aranarak ve çalışılan aile sağlığı merkezlerine gidilerek kendi özel numaraları veya mail adresleri alınmıştır. Google formlar aracılığıyla hazırlanan anket formu elektronik (WhatsApp veya mail aracılığı ile) olarak Samsun’daki aile sağlığı merkezlerinde çalışan aile hekimleri ve aile sağlığı elemanlarına ulaştırılmıştır. Kişiler gönüllülük esasına dayanılarak çalışmaya dahil edilmiştir. Bulgular: Katılımcıların %61,1’i kadın, %85,5’i evli, %55,7’si aile hekimiydi. Katılımcıların aşı tereddüdü olan ebeveynler ile karşılaşma sıklığı %75 olarak bulunmuştur. Aşı tereddüdünün en önemli nedeni, aşıların içeriğindeki maddeler olarak saptanmıştır. Sağlık çalışanlarına göre, aşı tereddüdünü azaltacak en önemli yöntem advers etkiler ile ilgili bilgilendirme yapılması olarak görülmüştür. Sonuç: Çalışmamızda, katılımcıların büyük bir çoğunluğu aşı tereddüdü olan en az bir aile ile daha önce karşılaştığını belirtmişlerdir. Sağlık çalışanlarına aşı tereddüdü ile ilgili eğitimlerin verilmesi fayda sağlayabilir.
Gebelerde Coronavirus ilişkili anksiyete sıklığı ve etkileyen faktörlerin değerlendirilmesi: Kesitsel çalışma
Authors: SÜNTAR Remzi , MERCAN BAŞPINAR Melike , SALMAN Süleyman , BASAT Okcan
Published: December 2022

Amaç: Araştırmamız, gebelerde Covid nedenli anksiyete sıklığı ve etkili faktörlerin tespit edilmesini amaçlamıştır. Yöntem: Gözlemsel (kesitsel) tipte olan çalışma Eylül 2020- Aralık 2020 tarihlerinde bir pandemi hastanesi Aile Hekimliği ve Gebe İzlem Polikliniklerine başvuran 1027 gebe ile gerçekleşmiştir. Katılımcılara demografik soru formu ve Covid anksiyete ölçeği uygulanmıştır. Covid anksiyetesi sıklığını arttıran risk faktörlerinin tespitinde lojistik regresyon testleri kullanılmıştır. Bulgular: Gebelerde, Covid anksiyete sıklığı %13,24 olup birinci, ikinci ve üçüncü trimesterde benzer oranda saptanmıştır (sırasıyla %14,0, %13,8, %12,4 ve p=0,783). Anksiyete ilgili en sık semptom %40,51 ile uyku bozukluğu olarak tespit edilmiştir. Ailede Covid pozitif birey olması 2,470 kat (p<0,001; %95GA:1,584-3,851), bilinen kronik hastalık varlığı 2,726 kat (p<0,001; %95GA:1,627-4,569) Covid nedenli anksiyete görülme sıklığını arttıran risk faktörü olarak izlenmiştir. Sonuç: Çalışmamızda, pandemi gibi beklenmedik acil bir durumda gebelerde nedene özel anksiyetenin belirleyicileri mevcut kronik hastalık varlığı ve yakınlarının etkene maruziyeti olmuştur. Bu durum gelecek yeni afet ve acil durumlarda gebe ruh sağlığında kontrol edilebilir risk faktörü olduğu için gebede kronik hastalık yönetimine dikkat çekmiştir.