bluuebunny/crossref_metadata_2025 huggingface dataset:
Hyperspectral Imaging to Study Dynamic Skin Perfusion after Injection of Articaine-4% with and without Epinephrine—Clinical Implications on Local Vasoconstriction
Authors: Thiem Daniel G. E. , Hans Lukas , Blatt Sebastian , Römer Paul , Heimes Diana , Al-Nawas Bilal , Kämmerer Peer W.
Published: August 2021

This study aimed to investigate the dynamic skin perfusion via hyperspectral imaging (HSI) after application of Articaine-4% ± epinephrine as well as epinephrine only. After the subcutaneous injection of (A100) Articaine-4% with epinephrine 1:100,000, (A200) Articaine-4% with epinephrine 1:200,000, (Aw/o) Articaine-4% without epinephrine, and (EPI200) epinephrine 1:200,000, into the flexor side of the forearm in a split-arm design, dynamic skin perfusion measurement was performed over 120 min by determining tissue oxygen saturation (StO2) using HSI. After injection, all groups experienced a reactive hyperaemia. With A200, it took about three min for StO2 to drop below baseline. For Aw/o and EPI200, perfusion reduction when compared to baseline was seen at 30 min with vasoconstriction >120 min. A100 caused vasodilation with hyperaemia >60 min. After three minutes, the perfusion pattern differed significantly (p < 0.001) between all groups except Aw/o and EPI200. The vasoactive effect of epinephrine-containing local anaesthetics can be visualised and dynamically quantified via StO2 using HSI. Aw/o + epinephrine 1:100,000 and 1:200,000 leads to perfusion reduction and tissue ischaemia after 30 min, which lasts over 120 min with no significant difference between both formulations. When using Aw/o containing epinephrine in terms of haemostasis for surgical procedures, a prolonged waiting time before incision of 30 or more min can be recommended.
Intraocular Temperature at Different Sites in Eye Measured at the Beginning of Vitreous Surgery
Authors: Shinoda Kei , Yagura Kazuma , Matsumoto Soiti , Terauchi Gaku , Mizota Atsushi , Miyake Yozo
Published: August 2021

The temperature of the vitreous has been reported to vary during cataract and vitreous surgery. We measured intraocular temperature at four intraocular sites; the anterior chamber (AC), just behind the crystalline lens, mid-vitreous, and just anterior to the optic disc (OD) at the beginning of vitrectomy with a thermoprobe in 48 eyes. The temperatures were compared in three groups; eyes that underwent vitrectomy for the first time (Group V, n = 30), eyes that had previous vitrectomy and the vitreous cavity had been filled with balanced salt solution (BSS; Group A, n = 12), and eyes that had previous vitrectomy and the vitreous cavity was filled with silicone oil (Group S, n = 6). There was a gradient in the temperature in all groups, i.e., it was lowest in the AC, and it increased at points closer to the retina. The intraocular temperature was significantly correlated with the type of fluid in the vitreous cavity. The mean intraocular temperatures were not significantly different in Groups V and A, but they were significantly higher in Group S. Clinicians should be aware of the differences in the temperature at the different intraocular sites because the temperatures may affect the physiology of the retina and the recovery process.
Association of TNF-α (-308G/A) Gene Polymorphism with Circulating TNF-α Levels and Excessive Daytime Sleepiness in Adults with Coronary Artery Disease and Concomitant Obstructive Sleep Apnea
Authors: Behboudi Afrouz , Thelander Tilia , Yazici Duygu , Celik Yeliz , Yucel-Lindberg Tülay , Thunström Erik , Peker Yüksel
Published: August 2021

Obstructive sleep apnea (OSA) is common in patients with coronary artery disease (CAD), in which inflammatory activity has a crucial role. The manifestation of OSA varies significantly between individuals in clinical cohorts; not all adults with OSA demonstrate the same set of symptoms; i.e., excessive daytime sleepiness (EDS) and/or increased levels of inflammatory biomarkers. The further exploration of the molecular basis of these differences is therefore essential for a better understanding of the OSA phenotypes in cardiac patients. In this current secondary analysis of the Randomized Intervention with Continuous Positive Airway Pressure in CAD and OSA (RICCADSA) trial (Trial Registry: ClinicalTrials.gov; No: NCT 00519597), we aimed to address the association of tumor necrosis factor alpha (TNF-α)-308G/A gene polymorphism with circulating TNF-α levels and EDS among 326 participants. CAD patients with OSA (apnea–hypopnea-index (AHI) ≥ 15 events/h; n = 256) were categorized as having EDS (n = 100) or no-EDS (n = 156) based on the Epworth Sleepiness Scale score with a cut-off of 10. CAD patients with no-OSA (AHI < 5 events/h; n = 70) were included as a control group. The results demonstrated no significant differences regarding the distribution of the TNF-α alleles and genotypes between CAD patients with vs. without OSA. In a multivariate analysis, the oxygen desaturation index and TNF-α genotypes from GG to GA and GA to AA as well as the TNF-α-308A allele carriage were significantly associated with the circulating TNF-α levels. Moreover, the TNF-α-308A allele was associated with a decreased risk for EDS (odds ratio 0.64, 95% confidence interval 0.41–0.99; p = 0.043) independent of age, sex, obesity, OSA severity and the circulating TNF-α levels. We conclude that the TNF-α-308A allele appears to modulate circulatory TNF-α levels and mitigate EDS in adults with CAD and concomitant OSA.
Post Approval Experience with Caplacizumab for Acquired Thrombotic Thrombocytopenic Purpura at a Single Institution
Authors: Logothetis Constantine N. , Patel Ankita , Eatrides Jennifer , Jaglal Michael , Haider Mintallah , Visweshwar Nathan , Laber Damian A.
Published: August 2021

Caplacizumab prevents platelet adhesion and has been approved for acquired thrombotic thrombocytopenic purpura (aTTP). This study was retrospective, including all patients diagnosed with aTTP and treated with caplacizumab since commercial availability in 2019 until 28 February 2021 at a single academic hospital with no exclusion criteria. Results used definitions for outcomes in aTTP from the International Working Group Consensus. Ten patients with aTTP received caplacizumab. The median age was 52 years. Six (60%) patients had refractory aTTP while 4 (40%) had newly diagnosed aTTP. The median laboratory values prior to therapy demonstrated: platelet count (PC) 29/uL, LDH 518 U/L (182–1850), ADAMTS13 activity 3% and ADAMTS13 inhibitor 1.4 BU. Everyone received glucocorticoids, rituximab, therapeutic plasma exchange (TPE) and caplacizumab. The median number of TPE was 12 days. Caplacizumab was started at a median of 5 days after the first TPE and the median treatment duration was 31 days. Normalization of PC, LDH and ADAMTS13 activity in days were 5, 3.5, and 32.5, respectively. Six (60%) patients achieved complete response, 3 (30%) had refractory disease and 1 (10%) had relapsed aTTP. No subject suffered abnormal bleeding, or thrombotic event. There were no deaths. Caplacizumab with TPE, glucocorticoids and rituximab was a safe and effective therapy for aTTP.
Effect of Progressive Head Extension Swallowing Exercise on Lingual Strength in the Elderly: A Randomized Controlled Trial
Authors: Park Jin-Woo , Oh Chi-Hoon , Choi Bo-Un , Hong Ho-Jin , Park Joong-Hee , Kim Tae-Yeon , Cho Yong-Jin
Published: August 2021

Lingual strengthening training can improve the swallowing function in older adults, but the optimal method is unclear. We investigated the effects of a new progressive resistance exercise in the elderly by comparing with a conventional isometric tongue strengthening exercise. Twenty-nine participants were divided into two groups randomly. One group performed forceful swallow of 2 mL of water every 10 s for 20 min, and a total of 120 swallowing tasks per session at 80% angle of maximum head extension. The other group performed five repetitions in 24 sets with a 30 s rest, and the target level was settled at 80% of one repetition maximum using the Iowa Oral Performance Instrument (IOPI). A total of 12 sessions were carried out by both groups over a 4-week period. Blinded measurements (for maximum lingual isometric pressure and peak pressure during swallowing) were obtained using IOPI before exercise and at four weeks in both groups. After four weeks, both groups showed a significant improvement in lingual strength involving both isometric and swallowing tasks. However, there was no significant difference between the groups in strength increase involving both tasks. Regardless of the manner, tongue-strengthening exercises substantially improved lingual pressure in the elderly with equal effect.
Improvement of Working Memory and Processing Speed in Patients over 70 with Bilateral Hearing Impairment Following Unilateral Cochlear Implantation
Authors: Knopke Steffen , Schubert Arvid , Häussler Sophia Marie , Gräbel Stefan , Szczepek Agnieszka J. , Olze Heidi
Published: August 2021

Several studies demonstrated the association of hearing disorders with neurocognitive deficits and dementia disorders, but little is known about the effects of auditory rehabilitation on the cognitive performance of the elderly. Therefore, the research question of the present study was whether cochlear implantation, performed in 21 patients over 70 with bilateral severe hearing impairment, could influence their cognitive skills. The measuring points were before implantation and 12 months after the first cochlear implant (CI) fitting. Evaluation of the working memory (WMI) and processing speed (PSI) was performed using the Wechsler Adult Intelligence Scale 4th edition (WAIS-IV). The audiological assessment included speech perception (SP) in quiet (Freiburg monosyllabic test; FMT), noise (Oldenburg sentence test; OLSA), and self-assessment inventory (Oldenburg Inventory; OI). Twelve months after the first CI fitting, not only the auditory parameters (SP and OI), but also the WMI and PSI, improved significantly (p < 0.05) in the cohort. The presented results imply that cochlear implantation of bilaterally hearing-impaired patients over 70 positively influences their cognitive skills.
Longitudinal Changes in Body Composition of Long-Term Survivors of Pancreatic Head Cancer and Factors Affecting the Changes
Authors: Kong Hyun-Ho , Kim Kyung-Won , Ko You-Sun , Kim Song-Cheol , Lee Jae-Hoon , Song Ki-Byung , Hwang Dae-Wook , Kim Won
Published: August 2021

Previous studies on changes in body composition of pancreatic cancer patients have only focused on short-term survivors. We studied longitudinal body composition changes and factors affecting them in long-term survivors by analyzing many abdominal computed tomography images using artificial intelligence technology. Of 302 patients who survived for >36 months after surgery were analyzed. Multivariate logistic regression analysis for factors affecting body composition changes and repeated-measures analysis of variance to observe differences in the course of change according to each factor were performed. In logistic analysis, preoperative sarcopenia and recurrence were the main factors influencing body composition changes at 1 and 3 years after surgery, respectively. In changes of longitudinal body composition, the decrease in body composition was the greatest at 3–6 months postoperatively, and the preoperative status did not recover even 3 years after surgery. Especially, males showed a greater reduction in skeletal muscle (SKM) after surgery than females (p < 0.01). In addition, SKM (p < 0.001) and subcutaneous adipose tissue (p < 0.05) mass rapidly decreased in case of recurrence. In conclusion, long-term survivors of pancreatic cancer did not recover their preoperative body composition status, and preoperative sarcopenia and recurrence influenced body composition changes.
Analyses of Swallowing Function and Its Related Factors in Community-Dwelling Elderly Patients: A Case-Control Study
Authors: Ogino Yoichiro , Suzuki Hiroki , Ayukawa Yasunori , Jinnouchi Akio , Koyano Kiyoshi
Published: August 2021

This retrospective case-control study evaluated the prevalence of declined swallowing function and the association with oral functions and gender in community-dwelling elderly patients. Their profiles, the results of swallowing function (Eating Assessment Tool: EAT-10) and other oral functions (oral dryness, maximum occlusal force (MOF), tongue–lip motor function (oral diadochokinesis: ODK), maximum tongue pressure (MTP) and masticatory performance (MP)) were extracted for analyses. The patients were categorized into three groups according to EAT-10 score (Group 1: 0, Group 2: 1 and 2, Group 3: ≥3). In total, 242 patients were enrolled and 46 of them (19.0%) were categorized into declined swallowing function (Group 3). In two-group comparisons (Group 1, 2 versus Group 3), significant differences were identified in age and the number of remaining teeth, but they were not identified in three-group comparisons. The patients with declined swallowing function (Group 3) had significantly lower function in ODK and MTP. Multiple logistic regression analyses identified that declined swallowing function was independently associated with declined functions in ODK /ka/ (OR: 5.31, 95% CI: 1.03–27.23, p = 0.04) and in MTP (OR: 2.74, 95% CI: 1.12–6.66, p = 0.03). This study could confirm the critical role of tongue functions in swallowing in community-dwelling elderly patients.
Green Nanocomposites for Energy Storage
Authors: Kausar Ayesha
Published: August 2021

The green nanocomposites have elite features of sustainable polymers and eco-friendly nanofillers. The green or eco-friendly nanomaterials are low cost, lightweight, eco-friendly, and highly competent for the range of energy applications. This article initially expresses the notions of eco-polymers, eco-nanofillers, and green nanocomposites. Afterward, the energy-related applications of the green nanocomposites have been specified. The green nanocomposites have been used in various energy devices such as solar cells, batteries, light-emitting diodes, etc. The main focus of this artifact is the energy storage application of green nanocomposites. The capacitors have been recognized as corporate devices for energy storage, particularly electrical energy. In this regard, high-performance supercapacitors have been proposed based on sustainable nanocomposites. Consequently, this article presents various approaches providing key knowledge for the design and development of multi-functional energy storage materials. In addition, the future prospects of the green nanocomposites towards energy storage have been discussed.
Morphological and Functional Changes of Roof Plate Cells in Spinal Cord Development
Authors: Shinozuka Takuma , Takada Shinji
Published: August 2021

The most dorsal region, or roof plate, is the dorsal organizing center of developing spinal cord. This region is also involved in development of neural crest cells, which are the source of migratory neural crest cells. During early development of the spinal cord, roof plate cells secrete signaling molecules, such as Wnt and BMP family proteins, which regulate development of neural crest cells and dorsal spinal cord. After the dorso-ventral pattern is established, spinal cord dynamically changes its morphology. With this morphological transformation, the lumen of the spinal cord gradually shrinks to form the central canal, a cavity filled with cerebrospinal fluid that is connected to the ventricular system of the brain. The dorsal half of the spinal cord is separated by a glial structure called the dorsal (or posterior) median septum. However, underlying mechanisms of such morphological transformation are just beginning to be understood. Recent studies reveal that roof plate cells dramatically stretch along the dorso-ventral axis, accompanied by reduction of the spinal cord lumen. During this stretching process, the tips of roof plate cells maintain contact with cells surrounding the shrinking lumen, eventually exposed to the inner surface of the central canal. Interestingly, Wnt expression remains in stretched roof plate cells and activates Wnt/β-catenin signaling in ependymal cells surrounding the central canal. Wnt/β-catenin signaling in ependymal cells promotes proliferation of neural progenitor and stem cells in embryonic and adult spinal cord. In this review, we focus on the role of the roof plate, especially that of Wnt ligands secreted by roof plate cells, in morphological changes occurring in the spinal cord.
Glaciers and Paleorecords Tell Us How Atmospheric Circulation Changes and Successive Cooling Periods Occurred in the Fennoscandia during the Holocene
Authors: Pinault Jean-Louis
Published: August 2021

Two major climatic phenomena that occurred during the Holocene are interpreted from the resonance in subharmonic modes of long-period Rossby waves winding around the North Atlantic gyre, the so-called gyral Rossby waves (GRWs). These are, on the one hand, the change in atmospheric circulation that occurred in the North Atlantic in the middle Holocene, and, on the other hand, the occurrence of abrupt cooling events more frequently than what is generally accepted. The amplitude of GRWs is deduced by filtering, within bands characteristic of various subharmonic modes, climate records from the Greenland ice sheet, pollen, and tree rings in northern Fennoscandia, and from two Norwegian glaciers in northern Folgefonna and on the Lyngen peninsula. While the subharmonic modes reflect the acceleration/deceleration phases of the western boundary current, an anharmonic mode is evidenced in the 400–450 year band. Abrupt cooling events of the climate are paced by this anharmonic mode while the western boundary current is decelerating, and the northward heat advection of air favors the melting of the pack ice. Then, the current of the northernmost part of the North Atlantic gyre cools before branching off to the north, which alters its buoyancy. On the other hand, according to high subharmonic modes, high-pressure systems prevailed over the North Atlantic in the first half of the Holocene while low-pressure systems resulted from baroclinic instabilities of the atmosphere dominate during the second half, favoring the growth of glaciers in Scandinavia by a better snowfall in winter and cooler summers.
Public Investment on Irrigation and Poverty Alleviation in Rural Laos
Authors: Inthakesone Bounmy , Syphoxay Pakaiphone
Published: August 2021

The demand for water is rising rapidly, particularly in agricultural and environmental sectors. This has led to more competition to access limited and scarce water resources. Therefore, choosing an appropriate approach to manage water resources, distribution and allocation, to attain sustainable agriculture is critical for every country worldwide. The most well-known method to preserve or store water and adaptation strategy to climate change is irrigation. This paper wished to understand the impact of irrigation on farmers’ income in Laos, especially from rice, which is the main crop of rural people. The difference in differences (DID) method was employed to estimate the regression results. The DID was estimated by the pooled OLS of the effect on the log of households’ rice farm income and log of households’ total income with household head’s age, education, gender, household size, ethnicity and harvest areas variables pointing out the coefficients of the outcome variables of interest (after treatment) were 0.037 and 0.076 with positive sign but statistically insignificant. The result implies irrigation has no impact on rice products. In other words, irrigation does not increase households’ income. The finding indicates the type of irrigation, the location of the operation headquarters and the management system or governance are crucial factors for explaining the impact of irrigation on the rice products in Laos.
Architectures and Protocols for Wireless Sensor and Actuator Networks
Authors: Di Marco Piergiuseppe , Park Pangun
Published: August 2021

Recent advances in wireless networking, sensing, computing, and control are revolutionizing how physical systems interact with information and physical processes such as Cyber-Physical Systems (CPS), Internet of Things (IoT), and Tactile Internet [...]
Developing a Metropolitan-Wide Urban Forest Strategy for a Large, Expanding and Densifying Capital City: Lessons from Melbourne, Australia
Authors: Hartigan Martin , Fitzsimons James , Grenfell Maree , Kent Toby
Published: August 2021

Urban forests provide many ecosystem services, such as reducing heat, improving air quality, treatment of stormwater, carbon sequestration, as well as biodiversity benefits. These benefits have resulted in increasing demand for urban forests and strategies to maintain and enhance this natural infrastructure. In response to a broader resilience strategy for Melbourne, Australia, we outline how a metropolitan-wide urban forest strategy (Living Melbourne) was developed, encompassing multiple jurisdictions and all land tenures. To this end, we mapped tree cover within the Melbourne metropolitan area, modelled potential habitat for some bird species, and investigated the role of tree cover for urban heat island mitigation. We outline the consultation and governance frameworks used to develop the strategy, the vision, goals and actions recommended, including canopy and shrub cover targets for different parts of the metropolitan area. The metropolitan-wide urban forest strategy acts as an overarching framework to guide local government authorities and various stakeholders towards a shared objective of increasing tree cover in Melbourne and we discuss the outcomes and lessons from this approach.
Psycholinguistic Evidence for Incipient Language Change in Mexican Spanish: The Extension of Differential Object Marking
Authors: Arechabaleta Regulez Begoña , Montrul Silvina
Published: August 2021

Spanish marks animate and specific direct objects overtly with the preposition a, an instance of Differential Object Marking (DOM). However, in some varieties of Spanish, DOM is advancing to inanimate objects. Language change starts at the individual level, but how does it start? What manifestation of linguistic knowledge does it affect? This study traced this innovative use of DOM in oral production, grammaticality judgments and on-line comprehension (reading task with eye-tracking) in the Spanish of Mexico. Thirty-four native speakers (ages 18–22) from the southeast of Mexico participated in the study. Results showed that the incidence of the innovative use of DOM with inanimate objects varied by task: DOM innovations were detected in on-line processing more than in grammaticality judgments and oral production. Our results support the hypothesis that language variation and change may start with on-line comprehension.
Retouchée au Féminin: The Gendered Nature of the French Law Mandating Labeling of Digitally Modified Images
Authors: Rodgers Rachel F. , Laveway Katherine
Published: August 2021

The majority of advertisements contain thin-ideal imagery that have been digitally modified. A robust body of research has suggested that exposure to these retouched images has negative effects on body image and increases eating disorder risk. Furthermore, these concerns are known to be highly gendered both in nature and in their extent, with women revealing higher levels of concerns predominantly related to thinness. Although not supported as a useful approach by empirical data, in 2017, France introduced a law requiring advertisers to label images featuring models whose weight and/or shape have been altered. These images must bear the label “photographie retouchée”, or “retouched image”. However, this legislation has been difficult to enforce, as unlike other French legislation related to labeling advertising, its lack of specificity makes it difficult to identify violations. Paradoxically, given its intentions, where applied, uses of the label disproportionately focus on women’s bodies in the media, as compared to men’s bodies. These findings highlight the need for legislation that is enforceable and supported by the allocation of sufficient resources. In addition, findings highlight the importance of grounding legislation and policy in the extant relevant data and involving strategic stakeholders in its creation.
Dystonia and Cerebellum: From Bench to Bedside
Authors: Morigaki Ryoma , Miyamoto Ryosuke , Matsuda Taku , Miyake Kazuhisa , Yamamoto Nobuaki , Takagi Yasushi
Published: August 2021

Dystonia pathogenesis remains unclear; however, findings from basic and clinical research suggest the importance of the interaction between the basal ganglia and cerebellum. After the discovery of disynaptic pathways between the two, much attention has been paid to the cerebellum. Basic research using various dystonia rodent models and clinical studies in dystonia patients continues to provide new pieces of knowledge regarding the role of the cerebellum in dystonia genesis. Herein, we review basic and clinical articles related to dystonia focusing on the cerebellum, and clarify the current understanding of the role of the cerebellum in dystonia pathogenesis. Given the recent evidence providing new hypotheses regarding dystonia pathogenesis, we discuss how the current evidence answers the unsolved clinical questions.
Unilateral Optic Nerve Sheath Fenestration in Idiopathic Intracranial Hypertension: A 6-Month Follow-Up Study on Visual Outcome and Prognostic Markers
Authors: Hagen Snorre Malm , Wegener Marianne , Toft Peter Bjerre , Fugleholm Kåre , Jensen Rigmor Højland , Hamann Steffen
Published: August 2021

Loss of vision is a feared consequence of idiopathic intracranial hypertension (IIH). Optic nerve sheath fenestration (ONSF) may be an effective surgical approach to protect visual function in medically refractory IIH. In this study, we evaluate the impact of unilateral superomedial transconjunctival ONSF on bilateral visual outcome using a comprehensive follow-up program. A retrospective chart review of IIH patients who underwent unilateral ONSF between January 2016 and March 2021 was conducted. Patients fulfilling the revised Friedman criteria for IIH and who had exclusively received ONSF as a surgical treatment were included. Main outcomes were visual acuity (VA); perimetric mean deviation (PMD); papilledema grade; and optic nerve head elevation (maxONHE) 1 week, 2 weeks, and 1, 3, and 6 months after surgery. VA (p < 0.05), PMD (p < 0.05), papilledema grade (p < 0.01), and maxOHNE (p < 0.001) were improved after 6 months on both the operated and non-operated eye. Prolonged surgical delay impedes PMD improvement (r = −0.78, p < 0.01), and an increasing opening pressure initiates a greater ganglion cell loss (r = −0.79, p < 0.01). In this small case series, we demonstrate that unilateral superonasal transconjunctival ONSF is a safe procedure with an effect on both eyes. Optic nerve head elevation and PMD are feasible biomarkers for assessing early treatment efficacy after ONSF.
Online Nonlinear Error Compensation Circuit Based on Neural Networks
Authors: Gao Zhenyi , Zhou Bin , Ju Chunge , Wei Qi , Zhang Xinxi , Zhang Rong
Published: August 2021

Nonlinear errors of sensor output signals are common in the field of inertial measurement and can be compensated with statistical models or machine learning models. Machine learning solutions with large computational complexity are generally offline or implemented on additional hardware platforms, which are difficult to meet the high integration requirements of microelectromechanical system inertial sensors. This paper explored the feasibility of an online compensation scheme based on neural networks. In the designed solution, a simplified small-scale network is used for modeling, and the peak-to-peak value and standard deviation of the error after compensation are reduced to 17.00% and 16.95%, respectively. Additionally, a compensation circuit is designed based on the simplified modeling scheme. The results show that the circuit compensation effect is consistent with the results of the algorithm experiment. Under SMIC 180 nm complementary metal-oxide semiconductor (CMOS) technology, the circuit has a maximum operating frequency of 96 MHz and an area of 0.19 mm2. When the sampling signal frequency is 800 kHz, the power consumption is only 1.12 mW. This circuit can be used as a component of the measurement and control system on chip (SoC), which meets real-time application scenarios with low power consumption requirements.
Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane
Authors: Choi Wanjae , Ryu Hyunil , Fuwad Ahmed , Goh Seulmini , Zhou Chaoge , Shim Jiwook , Takagi Masahiro , Kwon Soonjo , Kim Sun Min , Jeon Tae-Joon
Published: August 2021

Local anesthesia is a drug that penetrates the nerve cell membrane and binds to the voltage gate sodium channel, inhibiting the membrane potential and neurotransmission. It is mainly used in clinical uses to address the pain of surgical procedures in the local area. Local anesthetics (LAs), however, can be incorporated into the membrane, reducing the thermal stability of the membrane as well as altering membrane properties such as fluidity, permeability, and lipid packing order. The effects of LAs on the membrane are not yet fully understood, despite a number of previous studies. In particular, it is necessary to analyze which is the more dominant factor, the membrane affinity or the structural perturbation of the membrane. To analyze the effects of LAs on the cell membrane and compare the results with those from model membranes, morphological analysis and 50% inhibitory concentration (IC50) measurement of CCD-1064sk (fibroblast, human skin) membranes were carried out for lidocaine (LDC) and tetracaine (TTC), the most popular LAs in clinical use. Furthermore, the membrane affinity of the LAs was quantitatively analyzed using a colorimetric polydiacetylene assay, where the color shift represents their distribution in the membrane. Further, to confirm the membrane affinity and structural effects of the membranes, we performed an electrophysiological study using a model protein (gramicidin A, gA) and measured the channel lifetime of the model protein on the free-standing lipid bilayer according to the concentration of each LA. Our results show that when LAs interact with cell membranes, membrane affinity is a more dominant factor than steric or conformational effects of the membrane.
The Functional Significance of Hydrophobic Residue Distribution in Bacterial Beta-Barrel Transmembrane Proteins
Authors: Roterman Irena , Stapor Katarzyna , Fabian Piotr , Konieczny Leszek
Published: August 2021

β-barrel membrane proteins have several important biological functions, including transporting water and solutes across the membrane. They are active in the highly hydrophobic environment of the lipid membrane, as opposed to soluble proteins, which function in a more polar, aqueous environment. Globular soluble proteins typically have a hydrophobic core and a polar surface that interacts favorably with water. In the fuzzy oil drop (FOD) model, this distribution is represented by the 3D Gauss function (3DG). In contrast, membrane proteins expose hydrophobic residues on the surface, and, in the case of ion channels, the polar residues face inwards towards a central pore. The distribution of hydrophobic residues in membrane proteins can be characterized by means of 1–3DG, a complementary 3D Gauss function. Such an analysis was carried out on the transmembrane proteins of bacteria, which, despite the considerable similarities of their super-secondary structure (β-barrel), have highly differentiated properties in terms of stabilization based on hydrophobic interactions. The biological activity and substrate specificity of these proteins are determined by the distribution of the polar and nonpolar amino acids. The present analysis allowed us to compare the ways in which the different proteins interact with antibiotics and helped us understand their relative importance in the development of the resistance mechanism. We showed that beta barrel membrane proteins with a hydrophobic core interact less strongly with the molecules they transport.
Contraindications to the Initiation of Veno-Venous ECMO for Severe Acute Respiratory Failure in Adults: A Systematic Review and Practical Approach Based on the Current Literature
Authors: Harnisch Lars-Olav , Moerer Onnen
Published: August 2021

(1) Background: Extracorporeal membrane oxygenation (ECMO) is increasingly used for acute respiratory failure with few absolute but many relative contraindications. The provider in charge often has a difficult time weighing indications and contraindications to anticipate if the patient will benefit from this treatment, a decision that often decides life and death for the patient. To assist in this process in coming to a good evidence-based decision, we reviewed the available literature. (2) Methods: We performed a systematic review through a literature search of the MEDLINE database of former and current absolute and relative contraindications to the initiation of ECMO treatment. (3) Results: The following relative and absolute contraindications were identified in the literature: absolute—refusal of the use of extracorporeal techniques by the patient, advanced stage of cancer, fatal intracerebral hemorrhage/cerebral herniation/intractable intracranial hypertension, irreversible destruction of the lung parenchyma without the possibility of transplantation, and contraindications to lung transplantation; relative—advanced age, immunosuppressed patients/pharmacological immunosuppression, injurious ventilator settings > 7 days, right-heart failure, hematologic malignancies, especially bone marrow transplantation and graft-versus-host disease, SAPS II score ≥ 60 points, SOFA score > 12 points, PRESERVE score ≥ 5 points, RESP score ≤ −2 points, PRESET score ≥ 6 points, and “do not attempt resuscitation” order (DN(A)R status). (4) Conclusions: We provide a simple-to-follow algorithm that incorporates absolute and relative contraindications to the initiation of ECMO treatment. This algorithm attempts to weigh pros and cons regarding the benefit for an individual patient and hopefully assists caregivers to make better, informed decisions.
Membrane-Based Harvesting Processes for Microalgae and Their Valuable-Related Molecules: A Review
Authors: Castro-Muñoz Roberto , García-Depraect Octavio
Published: August 2021

The interest in microalgae production deals with its role as the third generation of feedstock to recover renewable energy. Today, there is a need to analyze the ultimate research and advances in recovering the microalgae biomass from the culture medium. Therefore, this review brings the current research developments (over the last three years) in the field of harvesting microalgae using membrane-based technologies (including microfiltration, ultrafiltration and forward osmosis). Initially, the principles of membrane technologies are given to outline the main parameters influencing their operation. The main strategies adopted by the research community for the harvesting of microalgae using membranes are subsequently addressed, paying particular attention to the novel achievements made for improving filtration performance and alleviating fouling. Moreover, this contribution also gives an overview of the advantages of applying membrane technologies for the efficient extraction of the high added-value compounds in microalgae cells, such as lipids, proteins and carbohydrates, which together with the production of renewable biofuels could boost the development of more sustainable and cost-effective microalgae biorefineries.
Modulation of the Human Erythroid Plasma Membrane Calcium Pump (PMCA4b) Expression by Polymorphic Genetic Variants
Authors: Mózner Orsolya , Zámbó Boglárka , Sarkadi Balázs
Published: August 2021

In the human ATP2B4 gene, coding for the plasma membrane calcium pump PMCA4b, a minor haplotype results in the decreased expression of this membrane protein in erythroid cells. The presence of this haplotype and the consequently reduced PMCA4b expression have been suggested to affect red blood cell hydration and malaria susceptibility. By using dual-luciferase reporter assays, we have localized the erythroid-specific regulatory region within the haplotype of the ATP2B4 gene, containing predicted GATA1 binding sites that are affected by SNPs in the minor haplotype. Our results show that, in human erythroid cells, the regulation of ATP2B4 gene expression is significantly affected by GATA1 expression, and we document the role of specific SNPs involved in predicted GATA1 binding. Our findings provide a mechanistic explanation at the molecular level for the reduced erythroid-specific PMCA4b expression in carriers of ATP2B4 gene polymorphic variants.
Using Reverse Osmosis Membrane at High Temperature for Water Recovery and Regeneration from Thermo-Responsive Ionic Liquid-Based Draw Solution for Efficient Forward Osmosis
Authors: Kamio Eiji , Kurisu Hiroki , Takahashi Tomoki , Matsuoka Atsushi , Yoshioka Tomohisa , Nakagawa Keizo , Matsuyama Hideto
Published: August 2021

Forward osmosis (FO) membrane process is expected to realize energy-saving seawater desalination. To this end, energy-saving water recovery from a draw solution (DS) and effective DS regeneration are essential. Recently, thermo-responsive DSs have been developed to realize energy-saving water recovery and DS regeneration. We previously reported that high-temperature reverse osmosis (RO) treatment was effective in recovering water from a thermo-responsive ionic liquid (IL)-based DS. In this study, to confirm the advantages of the high-temperature RO operation, thermo-sensitive IL-based DS was treated by an RO membrane at temperatures higher than the lower critical solution temperature (LCST) of the DS. Tetrabutylammonium 2,4,6-trimethylbenznenesulfonate ([N4444][TMBS]) with an LCST of 58 °C was used as the DS. The high-temperature RO treatment was conducted at 60 °C above the LCST using the [N4444][TMBS]-based DS-lean phase after phase separation. Because the [N4444][TMBS]-based DS has a significantly temperature-dependent osmotic pressure, the DS-lean phase can be concentrated to an osmotic pressure higher than that of seawater at room temperature (20 °C). In addition, water can be effectively recovered from the DS-lean phase until the DS concentration increased to 40 wt%, and the final DS concentration reached 70 wt%. From the results, the advantages of RO treatment of the thermo-responsive DS at temperatures higher than the LCST were confirmed.
Biaxial Experiments and Numerical Analysis on Stress-State-Dependent Damage and Failure Behavior of the Anisotropic Aluminum Alloy EN AW-2017A
Authors: Brünig Michael , Gerke Steffen , Koirala Sanjeev
Published: August 2021

Many experiments indicated the remarkable dependence of the strength and failure behavior of anisotropic ductile metals on the loading direction and on the stress state. These influences have to be taken into account in accurate material models and in the numerical simulation of complex loading processes predicting the safety and lifetime of aerospace structures. Therefore, the present paper discusses the effect of loading direction and stress state on the damage and failure behavior of the anisotropic aluminum alloy EN AW-2017A. Experiments and corresponding numerical analysis with the newly developed, biaxially loaded X0 specimen have been performed and the influence of different load ratios is examined. The formation of strain fields in critical parts of the X0 specimen is monitored by digital image correlation. Different failure modes are visualized by scanning electron microscopy of fracture surfaces. Stress states are predicted by finite element calculations and they are used to explain damage and fracture processes at the micro-level. The experimental–numerical analysis shows that the loading direction and the stress state remarkably affect the evolution of the width and orientation of localized strain fields as well as the formation of damage processes and fracture modes. As a consequence, characterization of anisotropic metals is highly recommended to be based on an enhanced experimental program with biaxial tests including different load ratios and loading directions.
Modern Powder Metallurgy: Chemical Composition Design for Improved Heat Resistant Alloys
Authors: Razumovskii Igor , Logacheva Alla , Razumovskiy Vsevolod , Logachev Ivan , Razumovsky Mikhail
Published: August 2021

The modern approach to the design of heat-resistant metal alloys (HRAs) is analyzed, according to which the creep rupture characteristics of an alloy are mostly determined by the strength of interatomic bonding at grain boundaries (GBs) and in the bulk of a matrix phase. The main attention is paid to the concept of “low alloying additions” to polycrystalline alloys with transition metals, because of which the cohesive strength of the GBs and the cohesion energy of the alloy matrix are increased. This approach is especially important in relation to alloys obtained by powder metallurgy, which, in the compacted state, are fine-grained polycrystals. The methodology for calculating the key parameters of the theory (the energy of impurity segregation to the grain boundaries Egb and to the free surface Efs, as well as the values of the partial molar energy of the cohesion of the alloys) from the first principles is given. The results of applying the theory to the study of Ni-, Cr- and Ti-based alloys and the development of new HRAs based on them are presented. Typical defects in the microstructures of objects obtained using additive technologies (AT) and the application efficiency of standard methods of processing powder alloys (Hot Isostatic Pressing (HIP), heat treatment (HT)) to improve the microstructure and increase the mechanical properties are considered.
News Trends in Powder Metallurgy: Microstructures, Properties, Durability
Authors: Hug Eric , Dirras Guy
Published: August 2021

Compared with traditional casting or plastic deformation processes, powder metallurgy-based methods are versatile routes for producing in-demand microstructures of various types [...]
Fatigue Behavior of Nonreinforced Hand-Holes in Aluminum Light Poles
Authors: Rusnak Cameron R. , Menzemer Craig C.
Published: August 2021

Hand-holes are present within the body of welded aluminum light poles. They are used to provide access to the electrical wiring for both installation and maintenance purposes. Wind is the main loading on these slender aluminum light poles and acts in a very cyclic way. In the field, localized fatigue cracking has been observed. This includes areas around hand-holes, most of which are reinforced with a cast insert welded to the pole. This study is focused on an alternative design, specifically hand-holes without reinforcement. Nine poles with 18 openings were fatigue tested in four-point bending at various stress ranges. Among the 18 hand-holes tested, 17 failed in one way or another as a result of fatigue cracking. Typically, fatigue cracking would occur at either the 3:00 or 9:00 positions around the hand-hole and then proceed to propagate transversely into the pole before failure. Finite element analysis was used to complement the experimental study. Models were created with varying aspect ratios to see if the hand-hole geometry had an effect on fatigue life.
Optimal Design of the Submerged Entry Nozzle for Thin Slab Continuous Casting Molds
Authors: Xuan Mingtao , Chen Min
Published: August 2021

For the purpose of increasing the capacity of an Angang Strip Production (ASP) continuous caster and the surface quality of a medium-thin slab with mold sections of 150 × (1020–1540) mm2, the present work investigated the influences of the submerged entry nozzle (SEN) structure and main operating parameters on the flow characteristic and temperature distribution in the mold by physical and numerical simulations. The results showed that the typical “double-roll” flow and a central jet were formed through the three-port SEN. With the original SEN, the mean wave height exceeded the critical value of 5.0 mm after the casting speed was increased due to the strong upper recirculation flow. By the slight increment of the bottom port area and the side port angle of SEN, the mean wave height was obviously decreased below 4.4 mm due to the depressing of the upper recirculation flow after the casting speed increased. Meanwhile, the temperature distribution was slightly changed by using the optimized SEN. The practical application showed that the breakout rate decreased from 0.349% to 0.107% and the surface defect rate decreased from 0.54% to 0.19% by using the optimized SEN, while throughput reached the new level of 3.96 t/min.
Effect of Milling Parameters on the Development of a Nanostructured FCC–TiNb15Mn Alloy via High-Energy Ball Milling
Authors: García-Garrido Cristina , Sepúlveda Ferrer Ranier , Salvo Christopher , García-Domínguez Lucía , Pérez-Pozo Luis , Lloreda-Jurado Pedro , Chicardi Ernesto
Published: August 2021

In this work, a blend of Ti, Nb, and Mn powders, with a nominal composition of 15 wt.% of Mn, and balanced Ti and Nb wt.%, was selected to be mechanically alloyed by the following two alternative high-energy milling devices: a vibratory 8000D mixer/mill® and a PM400 Retsch® planetary ball mill. Two ball-to-powder ratio (BPR) conditions (10:1 and 20:1) were applied, to study the evolution of the synthesized phases under each of the two mechanical alloying conditions. The main findings observed include the following: (1) the sequence conversion evolved from raw elements to a transitory bcc-TiNbMn alloy, and subsequently to an fcc-TiNb15Mn alloy, independent of the milling conditions; (2) the total full conversion to the fcc-TiNb15Mn alloy was only reached by the planetary mill at a minimum of 12 h of milling time, for either of the BPR employed; (3) the planetary mill produced a non-negligible Fe contamination from the milling media, when the highest BPR and milling time were applied; and (4) the final fcc-TiNb15Mn alloy synthesized presents a nanocrystalline nature and a partial degree of amorphization.
Effect of Calcium Treatment on Inclusions in H08A Welding Rod Steel
Authors: Lan Fangjie , Zhuang Changling , Li Changrong , Yang Guangkai , Yao Hanjie
Published: August 2021

The effect of calcium treatment on inclusions in H08A welding rod steel was studied by industrial experiment and using thermodynamics theory. The effects of inclusion composition, morphology, quantity, and size in H08A welding rod steel before and after calcium treatment were studied by metallographic microscope, scanning electron microscope (SEM), and energy dispersive spectrometer (EDS). Thermodynamic studies show that the addition of calcium can form various forms of xCaO·yAl2O3, under the condition that the composition of molten steel remains unchanged, the control of calcium content is the key to generate low melting point calcium-aluminate complex non-metallic inclusions and improve the quality of molten steel. The production practice in steel plant shows that for welding rod steels, the calcium content in a suitable range can meet the requirements of calcium treatment. Effective calcium treatment can not only transform the high melting point Al2O3 inclusions into the low melting point complex non-metallic inclusions between 3CaO·Al2O3 and 12CaO·7Al2O3, but also make the original shape-diversified inclusions into the spherical calcium-aluminate complex non-metallic inclusions. Meanwhile, the total number of inclusions and large-scale inclusions in welding rod steel are reduced, and the inclusions tend to disperse in the steel, which is very conducive to the improvement of steel quality. The results show that the modification path of magnesium aluminate spinel in steel is as follows: Al2O3 → MgO-Al2O3 → MgO-CaO-Al2O3. In addition, calcium treatment can modify MgO-Al2O3 spinel in steel into liquid MgO-CaO-Al2O3 complex non-metallic inclusions with low melting point.