bluuebunny/crossref_metadata_2025 huggingface dataset:
The Anti-Vitiligo Effects of Feshurin In Vitro from Ferula samarcandica and the Mechanism of Action
Authors: Nueraihemaiti Mayire , Deng Zang , Kamoldinov Khamidulla , Chao Niu , Habasi Maidina , Aisa Haji Akber
Published: September 2024

Background: Vitiligo is a complex disorder characterized by skin depigmentation; the canonical Wnt signaling pathway that involves β-catenin plays a crucial role in promoting the melanin production in melanocytes. Targeted inhibition of the Janus kinase JAK-STAT pathway can effectively diminish the secretion of the chemokine C-X-C motif ligand CXCL10, thereby safeguarding melanocytes. Ferula has been applied as a treatment regimen for a long period; however, its use for the treatment of vitiligo has not been previously documented. Methods: CCK-8 assay, Intracellular melanin content assay, Tyrosinase activity assay, Western blotting, qRT-PCR, and ELISA methods were employed. Using molecular docking verified the inhibitory effects of feshurin on the JAK1. Results: The sesquiterpene coumarin feshurin was separated from Ferula samarcandica. Feshurin was shown to induce GSK-3β phosphorylation, resulting in the translocation of β-catenin into the nucleus. This translocation subsequently upregulated the transcription of microphthalmia-associated transcription factor (MITF), leading to increased tyrosinase activity and melanin production. In addition, feshurin inhibited the production of chemokine CXCL10 via the JAK-STAT signaling pathway, which was verified by molecular docking. Conclusions: Based on these findings, it can be concluded that feshurin exhibits significant potential for the development of novel anti-vitiligo therapeutics.
Analysis of Lipophilicity and Pharmacokinetic Parameters of Dipyridothiazine Dimers with Anticancer Potency
Authors: Martula Emilia , Morak-Młodawska Beata , Jeleń Małgorzata , Okechukwu Patrick Nwabueze
Published: September 2024

Lipophilicity is an essential parameter of a compound that determines the solubility and pharmacokinetic properties that determine the transport of the drug to the molecular target. Dimers of dipyridothiazines are diazaphenothiazine derivatives exhibiting diverse anticancer potential in vitro, which is related to their affinity for histone deacetylase. In this study, the lipophilicity of 16 isomeric dipyridothiazine dimers was investigated theoretically and experimentally by reversed-phase thin-layer chromatography (RP-TLC) in an acetone–TRIS buffer (pH = 7.4). The relative lipophilicity parameter RM0 and specific hydrophobic surface area b were significantly intercorrelated, showing congeneric classes of dimers. The parameter RM0 was transformed into parameter logPTLC by use of the calibration curve. Molecular descriptors, ADMET parameters and probable molecular targets were determined in silico for analysis of the pharmacokinetic profile of the tested compounds showing anticancer activity. The analyzed compounds were tested in the context of Lipinski’s rule of five, Ghose’s rule and Veber’s rule, confirming their bioavailability.
Genome-Wide Association for Morphological and Agronomic Traits in Phaseolus vulgaris L. Accessions
Authors: Alves Stephanie Mariel , Lacanallo Giselly Figueiredo , Gonçalves-Vidigal Maria Celeste , Vaz Bisneta Mariana , Vidigal Rosenberg Andressa Gonçalves , Vidigal Filho Pedro Soares
Published: September 2024

Exploring genetic resources through genomic analyses has emerged as a powerful strategy to develop common bean (Phaseolus vulgaris L.) cultivars that are both productive and well-adapted to various environments. This study aimed to identify genomic regions linked to morpho-agronomic traits in Mesoamerican and Andean common bean accessions and to elucidate the proteins potentially involved in these traits. We evaluated 109 common bean accessions over three agricultural years, focusing on traits including the grain yield (YDSD), 100-seed weight (SW), number of seeds per pod (SDPD), number of pods per plant (PDPL), first pod insertion height (FPIH), plant height (PLHT), days to flowering (DF), and days to maturity (DPM). Using multilocus methods such as mrMLM, FASTmrMLM, FASTmrEMMA, ISIS EM-BLASSO, and pLARmEB, we identified 36 significant SNPs across all chromosomes (Pv01 to Pv11). Validating these SNPs and candidate genes in segregating populations is crucial for developing more productive common bean cultivars through marker-assisted selection.
Rhizofungus Aspergillus terreus Mitigates Heavy Metal Stress-Associated Damage in Triticum aestivum L.
Authors: Dilawar Naveen , Hamayun Muhammad , Iqbal Amjad , Lee Bokyung , Ali Sajid , Ahmad Ayaz , Alrefaei Abdulwahed Fahad , Faraj Turki Kh. , Kim Ho-Youn , Hussain Anwar
Published: September 2024

Industrial waste and sewage deposit heavy metals into the soil, where they can remain for long periods. Although there are several methods to manage heavy metals in agricultural soil, microorganisms present a promising and effective solution for their detoxification. We isolated a rhizofungus, Aspergillus terreus (GenBank Acc. No. KT310979.1), from Parthenium hysterophorus L., and investigated its growth-promoting and metal detoxification capabilities. The isolated fungus was evaluated for its ability to mitigate lead (25 and 75 ppm) and copper (100 and 200 ppm) toxicity in Triticum aestivum L. seedlings. The experiment utilized a completely randomized design with three replicates for each treatment. A. terreus successfully colonized the roots of wheat seedlings, even in the presence of heavy metals, and significantly enhanced plant growth. The isolate effectively alleviates lead and copper stress in wheat seedlings, as evidenced by increases in shoot length (142%), root length (98%), fresh weight (24%), dry weight (73%), protein content (31%), and sugar content (40%). It was observed that wheat seedlings possess a basic defense system against stress, but it was insufficient to support normal growth. Fungal inoculation strengthened the host’s defense system and reduced its exposure to toxic heavy metals. In treated seedlings, exposure to heavy metals significantly upregulated MT1 gene expression, which aided in metal detoxification, enhanced antioxidant defenses, and maintained metal homeostasis. A reduction in metal exposure was observed in several areas, including normalizing the activities of antioxidant enzymes that had been elevated by up to 67% following exposure to Pb (75 mg/kg) and Cu (200 mg/kg). Heavy metal exposure elevated antioxidant levels but also increased ROS levels by 86%. However, with Aspergillus terreus colonization, ROS levels stayed within normal ranges. This decrease in ROS was associated with reduced malondialdehyde (MDA) levels, enhanced membrane stability, and restored root architecture. In conclusion, rhizofungal colonization improved metal tolerance in seedlings by decreasing metal uptake and increasing the levels of metal-binding metallothionein proteins.
Effects of Acmella radicans Invasion on Soil Seed Bank Community Characteristics in Different Habitats
Authors: Wu Xiaohan , Yang Kexin , Zheng Fengping , Xu Gaofeng , Fan Zewen , Clements David Roy , Yang Yunhai , Yang Shaosong , Jin Guimei , Zhang Fudou , Shen Shicai
Published: September 2024

To examine the effects of the recent Acmella radicans invasion on plant community and diversity in invaded habitats, the composition, density, species richness, diversity indices, and evenness index of the soil seed bank community of two different habitats (wasteland and cultivated land) in Yunnan Province, China, were analyzed through field sampling and greenhouse germination tests. A total of 28 species of plants belonging to 15 families and 28 genera, all annual herbs, were found in the soil seed bank. Seed densities and species number in the seed bank tended to be greater in April than in October; cultivated land also featured higher seed densities and species numbers compared to wasteland. With increased A. radicans cover, the seed bank population of A. radicans also significantly increased, but the seed bank populations of many other dominant species (e.g., Ageratum conyzoides and Gamochaeta pensylvanica) and native species (e.g., Laggera crispata and Poa annua) clearly declined. The germination of A. radicans seeds was concentrated during the period from the 4th to the 5th weeks. Vertically, the seed number of A. radicans was significantly different among the 0–5 cm, 5–10 cm and 10–20 cm layers that accounted for 80.7–90.6%, 9.4–16.1% and 0.0–3.2% of the total seed density in wasteland, respectively; and in cultivated land, A. radicans accounted for 56.8–64.9%, 26.7–31.8% and 8.1–13.5% of the total seed density, respectively. With reduced A. radicans cover, the species richness, Simpson index, Shannon–Wiener index, and Pielou indices of the weed community generally increased, and most diversity indices of weed communities in cultivated land were lower than in wasteland under the same cover of A. radicans. The results indicate that the invasion of A. radicans has negatively affected local weed community composition and reduced weed community diversity, and that these negative impacts in cultivated land may be enhanced by human disturbance. Our study was the first to elucidate the influence of A. radicans invasion on soil seed bank community characteristics in invaded habitats, providing a better understanding of its invasion and spread mechanisms in order to aid in developing a scientific basis for the prevention and control of this invader.
Functional Identification and Regulatory Active Site Screening of the DfDXS Gene of Dryopteris fragrans
Authors: Zhao Hanxu , Su Jiameng , Zhong Zhaoxuan , Xiong Tongyou , Dai Weicong , Zhang Dongrui , Chang Ying
Published: September 2024

Dryopteris fragrans (L.) Schott has anti-inflammatory and antioxidant properties, and terpenoids are important components of its active constituents. The methyl-D-erythritol 4-phosphate (MEP) pathway is one of the major pathways for the synthesis of terpene precursors in plants, and 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in this pathway. DXS has been shown to be associated with increased stress tolerance in plants. In this experiment, two DXS genes were extracted from the D. fragrans transcriptome and named DfDXS1 and DfDXS2. Based on phylogenetic tree and conserved motif analyses, DXS was shown to be highly conserved evolutionarily and its localization to chloroplasts was determined by subcellular localization. Prokaryotic expression results showed that the number and growth status of recombinant colonies were better than the control under 400 mM NaCl salt stress and 800 mM mannitol-simulated drought stress. In addition, the DfDXS1 and DfDXS2 transgenic tobacco plants showed improved resistance to drought and salt stress. DfDXS1 and DfDXS2 responded strongly to methyl jasmonate (MeJA) and PEG-mimicked drought stress following exogenous hormone and abiotic stress treatments of D. fragrans. The transcriptional active sites were investigated by dual luciferase and GUS staining assays, and the results showed that the STRE element (AGGGG), the ABRE element (ACGTGGC), and the MYC element (CATTTG) were the important transcriptional active sites in the promoters of the two DXS genes, which were closely associated with hormone response and abiotic stress. These results suggest that the DfDXS gene of D. fragrans plays an important role in hormone signaling and response to stress. This study provides a reference for analyzing the molecular mechanisms of stress tolerance in D. fragrans.
Impact of Chromosomal Fusion and Transposable Elements on the Genomic Evolution and Genetic Diversity of Ilex Species
Authors: Xu Zhenxiu , Wei Haikun , Li Mingyue , Qiu Yingjie , Li Lei , Xu Ke-Wang , Guo Zhonglong
Published: September 2024

The genus Ilex belongs to the sole family and is the single genus within the order Aquifoliales, exhibiting significant phenotypic diversity. However, the genetic differences underlying these phenotypic variations have rarely been studied. In this study, collinearity analyses of three Ilex genomes, Ilex latifolia Thunb., Ilex polyneura (Hand.-Mazz.) S. Y. Hu, and Ilex asprella Champ. ex Benth., indicated a recent fusion event contributing to the reduction of chromosomes in I. asprella. Comparative genome analyses showed slight differences in gene annotation among the three species, implying a minimal disruption of genes following chromosomal fusion in I. asprella. Comprehensive annotation of transposable elements (TEs) revealed that TEs constitute a significant portion of the Ilex genomes, with LTR transposons being predominant. TEs exhibited an inverse relationship with gene density, potentially influencing gene regulation and chromosomal architecture. TE insertions were shown to affect the conformation and binding sites of key genes such as 7-deoxyloganetin glucosyltransferase and transmembrane kinase (TMK) genes, highlighting potential functional impacts. The structural variations caused by TE insertions suggest significant roles in the evolutionary dynamics, leading to either loss or gain of gene function. This study underscores the importance of TEs in shaping the genomic landscape and evolutionary trajectories of Ilex species.
Comparative Analysis of Water Stress Regimes in Avocado Plants during the Early Development Stage
Authors: Rondon Tatiana , Guzmán-Hernández Manuel , Torres-Madronero Maria C. , Casamitjana Maria , Cano Lucas , Galeano July , Goez Manuel
Published: September 2024

The avocado cv. Hass requires a suitable rootstock for optimal development under water stress. This study evaluated the performance of two avocado rootstocks (ANRR88 and ANGI52) grafted onto cv. Hass under four water stress conditions, 50% and 25% deficit, and 50% and 25% excess during the nursery stage. Plant height, leaf area (LA), dry matter (DM), and Carbon (OC) content in the roots, stems, and leaves were measured. Root traits were evaluated using digital imaging, and three vegetation indices (NDVI, CIRE, and MTCI) were used to quantify stress. The results showed that genotype significantly influenced the response to water stress. ANRR88 exhibited adaptation to moderate to high water deficits. ANGI52 adapted better to both water deficit and excess, and showed greater root exploration. LA and DM reductions of up to 60% were observed in ANRR88, suggesting a higher sensitivity to extreme changes in water availability. More than 90% of the total OC accumulation was observed in the stem and roots. The NDVI and the MTCI quantified the presence and levels of stress applied, and the 720 nm band provided high precision and speed for detecting stress. These insights are crucial for selecting rootstocks that ensure optimal performance under varying water availability, enhancing productivity and sustainability.
Chitosan-Polyaniline (Bio)Polymer Hybrids by Two Pathways: A Tale of Two Biocomposites
Authors: Anisimov Yuriy A. , Yang Heng , Kwon Johnny , Cree Duncan E. , Wilson Lee D.
Published: September 2024

Previous research highlights the potential of polyaniline-based biocomposites as unique adsorbents for humidity sensors. This study examines several preparative routes for creating polyaniline (PANI) and chitosan (CHT) composites: Type 1—in situ polymerization of aniline with CHT; Type 2—molecular association in acidic aqueous media; and a control, Type 3—physical mixing of PANI and CHT powders (without solvent). The study aims to differentiate the bonding nature (covalent vs. noncovalent) within these composites, which posits that noncovalent composites should exhibit similar physicochemical properties regardless of the preparative route. The results indicate that Type 1 composites display features consistent with covalent and hydrogen bonding, which result in reduced water swelling versus Type 2 and 3 composites. These findings align with spectral and thermogravimetric data, suggesting more compact structure for Type 1 materials. Dye adsorption studies corroborate the unique properties for Type 1 composites, and 1H NMR results confirm the role of covalent bonding for the in situ polymerized samples. The structural stability adopts the following trend: Type 1 (covalent and noncovalent) > Type 2 (possible trace covalent and mainly noncovalent) > Type 3 (noncovalent). Types 2 and 3 are anticipated to differ based on solvent-driven complex formation. This study provides greater understanding of structure-function relationships in PANI-biopolymer composites and highlights the role of CHT as a template that involves variable (non)covalent contributions with PANI, according to the mode of preparation. The formation of composites with tailored bonding modalities will contribute to the design of improved adsorbent materials for environmental remediation to versatile humidity sensor systems.
Construction of Thick Myocardial Tissue through Layered Seeding in Multi-Layer Nanofiber Scaffolds
Authors: You Yuru , Xu Feng , Liu Lingling , Chen Songyue , Ding Zhengmao , Sun Daoheng
Published: September 2024

A major challenge in myocardial tissue engineering is replicating the heart’s highly complex three-dimensional (3D) anisotropic structure. Heart-on-a-chip (HOC) is an emerging technology for constructing myocardial tissue in vitro in recent years, but most existing HOC systems face difficulties in constructing 3D myocardial tissue aligned with multiple cell layers. Electrospun nanofibers are commonly used as scaffolds for cell growth in myocardial tissue engineering, which can structurally simulate the extracellular matrix to induce the aligned growth of myocardial cells. Here, we developed an HOC that integrates multi-layered aligned polycaprolactone (PCL) nanofiber scaffolds inside microfluidic chips, and constructed 3D thick and aligned tissue with a layered seeding approach. By culturing human-induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) on chip, the myocardial tissue on the two layered nanofibers reached a thickness of ~53 μm compared with ~19 μm for single-layered nanofibers. The obtained myocardial tissue presented well-aligned structures, with densely distributed α-actinin. By the third day post seeding, the hiPSC-CMs contract highly synchronously, with a contraction frequency of 18 times/min. The HOC with multi-layered biomimetic scaffolds provided a dynamic in vitro culture environment for hiPSC-CMs. Together with the layered cell-seeding process, the designed HOC promoted the formation of thick, well-aligned myocardial tissue.
Correction: Rehman et al. Nanocomposite Membranes for PEM-FCs: Effect of LDH Introduction on the Physic-Chemical Performance of Various Polymer Matrices. Polymers 2023, 15, 502
Authors: Rehman Muhammad Habib Ur , Lufrano Ernestino , Simari Cataldo
Published: September 2024

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A Numerical Study on the Flow Field and Classification Performance of an Industrial-Scale Micron Air Classifier under Various Outlet Mass Airflow Rates
Authors: Ho Nang Xuan , Dinh Hoi Thi , Dau Nhu The , Nguyen Bang Hai
Published: September 2024

In this study, the gas−particle flow field in a real-size industrial-scale micron air classifier manufactured by Phenikaa Group using 3D transient simulations with the FWC-RSM–DPM (Four-Way Coupling-Reynold Stress Model-Discrete Phase Model) in ANSYS Fluent 2022 R2 and with the assistance of High-Performance Computing (HPC) systems is explored. A comparison among three coupling models is carried out, highlighting the significant influence of the interactions between solid and gas phases on the flow field. The complex two-phase flow, characterized by the formation of multiple vortices with different sizes, positions, and rotation directions, is successfully captured on the real-size model of the classifier. Additionally, analyzing the effects of the vortices on the flow field provides a comprehensive understanding of the gas–solid flow field and the classification mechanism. The effect of the outlet mass airflow rate is also investigated. The classifier’s Key Performance Indicators (KPIs: d50, K, η, ΔP) and the constrained condition of the particle size distribution curve of the final product are used to evaluate the classification efficiency. The contributions of this work are as follows: (i) a simulation analysis of a real-size industrial-scale classifier is conducted that highlights its advantages over a lab-scale one; (ii) a comparison is conducted among three coupling models, showing the advancement of four-way coupling in providing accurate results for simulations of interactions between the gas phase and particles; and (iii) the particle size distribution curve performances of a classified product under different simulation models and outlet airflow rates are addressed, from which optimal parameters can be selected in the design and operation processes to achieve the required efficiency of an air classifier.
Study of Noise Reduction and Identification of Internal Damage Signals in Wire Ropes
Authors: Li Pengbo , Tian Jie
Published: September 2024

Mining wire rope, a frequently used load-bearing element, suffers various forms of damage over extended periods of operation. Damage occurring within the wire rope, which is not visible to the naked eye and is difficult to detect accurately with current technology, is of particular concern. Consequently, the identification of internal damage assumes paramount importance in ensuring mine safety. This study proposes a wire rope internal damage noise reduction and identification method, first of all, through a three-dimensional magnetic dipole model to achieve the detection and analysis of the internal damage of the wire rope. Simultaneously, a sensor system capable of accurately detecting the internal damage of wire rope is developed and validated through experimentation. A novel approach is proposed to address the noise reduction issue in the design process. This approach utilizes a particle swarm optimization variational modal decomposition method to enhance the signal-to-noise ratio. Additionally, a dual-attention mode, which combines channel attention and spatial attention, is integrated into the CNN-GRU network model. This network model is specifically designed for the detection of internal damage in steel wire ropes. The proposed method successfully achieves quantitative identification of internal damage in steel wire ropes. The experimental findings demonstrate that this approach is capable of efficiently detecting internal damage in wire rope and possesses the capacity to quantitatively identify such damage, enabling adaptive identification of wire rope.
The Microstructure, Mechanical Properties, and Precipitation Behavior of 1000 MPa Grade GEN3 Steel after Various Quenching Processes
Authors: Ning Angang , Gao Rui , Yue Stephen , Skszek Timothy
Published: September 2024

This study examines the microstructure, mechanical properties, and precipitation behavior of 1000 MPa grade GEN3 steel when subjected to various quenching processes, with a focus on the quench and partition (Q&P) technique. The Q&P-treated samples achieved 1300 MPa tensile strength and demonstrated superior yield strength, attributed to their refined substructure and their large amounts of precipitates. The quenched samples exhibited the thinnest martensite laths due to the highest martensite volume. Despite the as-annealed samples having the smallest grain size, the Q&P treatment resulted in optimal microstructural refinement results and a high dislocation density, reaching 1.15 × 1015 m−2. Analysis of the precipitates revealed the presence of V8C7, M7C3, M2C, and Ti(C, N) across various heat treatments. The application of the McCall–Boyd method and the Ashby–Orowan correction model indicated that quench and tempered (Q&T) samples contained the largest volume of fine precipitates, contributing to their high yield strengths. These findings offer valuable insights for optimizing heat treatment processes to develop advanced high-strength steels for industrial applications.
Optimal Reactive Power Dispatch and Demand Response in Electricity Market Using Multi-Objective Grasshopper Optimization Algorithm
Authors: Das Punam , Dawn Subhojit , Gope Sadhan , Das Diptanu , Salata Ferdinando
Published: September 2024

Optimal Reactive Power Dispatch (ORPD) is a power system optimization tool that modifies system control variables such as bus voltage and transformer tap settings, and it compensates devices’ Volt Ampere Reactive (VAR) output. It is used to decrease real power loss, enhance the voltage profile, and promote stability. Furthermore, several issues have been faced in electricity markets, such as price volatility, transmission line congestion, and an increase in the cost of electricity during peak hours. Programs such as demand response (DR) provide system operators with more control over how small customers participate in lowering peak-hour energy prices and demand. This paper presents an extensive study on ORPD methodologies and DR programs for lowering voltage deviation, limiting cost, and minimizing power losses to create effective and economical operations systems. The main objectives of this work are to minimize costs and losses in the system and reduce voltage variation. The Grasshopper Optimization Algorithm (GOA) and Dragonfly Algorithm (DA) have been implemented successfully to solve this problem. The proposed technique has been evaluated by using the IEEE-30 bus system. The results obtained by the implementation of demand response systems show a considerable reduction in costs and load demands that benefit consumers through DR considerations. The results obtained from the GOA and DA are compared with those generated by other researchers and published in the literature to ascertain the algorithm’s efficiency.
A Method for Predicting the Action Sites of Regional Mudstone Cap Rock Affecting the Diversion of Hydrocarbons Transported along Oil Source Faults
Authors: Zhou Tianqi , Wang Yachun , Yuan Hongqi , Yu Yinghua , Zhang Yunfeng
Published: September 2024

Regional mudstone cap rock has an important influence on the oil and gas distribution of the oil source faults below it. Therefore, studying the influence of these mudstone cap rocks on the hydrocarbon distribution pattern is fundamental to understanding the oil and gas distribution of the lower generation and upper reservoir reservoirs in the Bohai Bay Basin. This study classified two types of hydrocarbon diversion from oil source faults: blockage diversion and seepage diversion. To locate them, we established a method to predict the areas with blockage diversion and seepage diversion separately by superimposing the sealing and leakage parts of the regional mudstone cap rock with the regions of the connected distribution of sand bodies and the favorable hydrocarbon transport sites of the oil source faults, respectively. We used this approach to predict the locations where hydrocarbons are diverted by the oil source faults under the regional mudstone cap rocks in the first and second sections of the Dongying Formation (E3d1-2) in the Liuchu area of the Raoyang Sag, Bohai Bay Basin. The results show that the regional mudstone cap rock’s blockage diversion occurs mainly in the south-central area of Liuchu, with a localized distribution in the northern part. The seepage diversion site is primarily located in the northeastern area and is also found locally in the west. Both diversions are beneficial for the accumulation of hydrocarbons from the source rocks of the first member of the Shahejie Formation (E3s1) to the upper second member of the Dongying Formation (E3d2U). The latter can also accumulate hydrocarbons in the Guantao Formation (N1g). The results align with the hydrocarbon distribution, demonstrating the feasibility of our method to predict various oil source fault diversion sites under the regional mudstone cap rock. This prediction method offers valuable guidance for exploring the lower generation and upper reservoir hydrocarbon accumulations in hydrocarbon-bearing basins.
God as Male–Female: Priscillian, Prophecy, and the Witness of Irenaeus and Marius Victorinus
Authors: Mews Constant J.
Published: September 2024

This paper examines a comment by Priscillian (d. c. 385) in his Liber apologeticus that certain people erroneously applied to God the unusual Latin neologism, masculofemina. He contrasts their perspective with scriptural teaching about the Holy Spirit being poured out on both men and women. This raises two questions, namely, how Priscillian’s comment relates to accusations he faced of encouraging dangerous intimacy between men and women and the source of his information about their teaching. This paper argues that the central thrust of Priscillian’s teaching is around the notion that the spirit of prophecy was manifested in both sexes, but that he distinguished his teaching from that of Valentinian gnostics to defend his own orthodoxy. It argues that Priscillian acquired this teaching about God as masculofemina from the translation into Latin of the Aduersus haereses of Irenaeus of Lyons (d. c. 202). The term also occurs within the writing of Marius Victorinus (c. 359–61) in defense of Catholic Christianity. Priscillian drew on Irenaeus to defend the orthodoxy of his notion that the gift of prophecy was given to both men and women.
Southern Baptist Slaveholding Women and Mythologizers
Authors: Vaughn Cross C. A.
Published: September 2024

Christian slaveholding should not be forgotten or minimized, nor should its mythologies go unchallenged or uncritiqued. This article surveys some of the leading Southern Baptist women slaveholders and mythologizers before and after the U.S. Civil War. It examines sources of SBC hagiography about the Convention foremothers and their persistent apologia for slaveholding. In particular, it discusses how female mythologizers in the antebellum and postbellum eras linked slaveholding, evangelism, and mission identity. It demonstrates how postbellum Southern Baptist women chose to view women slaveholders as moral exemplars for their current missions. It concludes that understanding the myth-making by and about women slaveholders in Southern Baptist patriarchal society is instructive for understanding this group of American Evangelical Protestants in Christian history.
Swarm Investigation of Ultra-Low-Frequency (ULF) Pulsation and Plasma Irregularity Signatures Potentially Associated with Geophysical Activity
Authors: Balasis Georgios , De Santis Angelo , Papadimitriou Constantinos , Boutsi Adamantia Zoe , Cianchini Gianfranco , Giannakis Omiros , Potirakis Stelios M. , Mandea Mioara
Published: September 2024

Launched on 22 November 2013, Swarm is the fourth in a series of pioneering Earth Explorer missions and also the European Space Agency’s (ESA’s) first constellation to advance our understanding of the Earth’s magnetic field and the near-Earth electromagnetic environment. Swarm provides an ideal platform in the topside ionosphere for observing ultra-low-frequency (ULF) waves, as well as equatorial spread-F (ESF) events or plasma bubbles, and, thus, offers an excellent opportunity for space weather studies. For this purpose, a specialized time–frequency analysis (TFA) toolbox has been developed for deriving continuous pulsations (Pc), namely Pc1 (0.2–5 Hz) and Pc3 (22–100 mHz), as well as ionospheric plasma irregularity distribution maps. In this methodological paper, we focus on the ULF pulsation and ESF activity observed by Swarm satellites during a time interval centered around the occurrence of the 24 August 2016 Central Italy M6 earthquake. Due to the Swarm orbit’s proximity to the earthquake epicenter, i.e., a few hours before the earthquake occurred, data from the mission may offer a variety of interesting observations around the time of the earthquake event. These observations could be associated with the occurrence of this geophysical event. Most notably, we observed an electron density perturbation occurring 6 h prior to the earthquake. This perturbation was detected when the satellites were flying above Italy.
Analysis of Spatiotemporal Changes in Energy Consumption Carbon Emissions at District and County Levels Based on Nighttime Light Data—A Case Study of Jiangsu Province in China
Authors: Xiang Chengzhi , Mei Yong , Liang Ailin
Published: September 2024

Approximately 86% of the total carbon emissions are generated by energy consumption, and the study of the variation of energy consumption carbon emissions (ECCE) is of vital significance to regional sustainable development and energy conservation. Currently, carbon emissions accounting mainly focuses on large and medium-scale statistics, but at smaller scales (district and county level), it still remains unclear. Due to the high correlation between nighttime light (NTL) data and ECCE, this study combines “energy inventory statistics” with NTL data to estimate ECCE at smaller scales. First, we obtained city-level statistics on ECCE and corrected the NTL data by applying the VANUI index to the original NTL data from NPP-VIIRS. Second, an analysis was conducted on the correlation between the two variables, and a model was created to fit the relationship between them. Under the assumption that ECCE will be consistent within a given region, we utilized the model to estimate ECCE in districts and counties, eventually obtaining correct results at the county-level. We estimated the ECCE in each district and county of Jiangsu Province from 2013 to 2022 using the above-proposed approach, and we examined the variations in these emissions both spatially and temporally across the districts and counties. The results revealed a significant degree of correlation between the two variables, with the R2 of the fitting models exceeding 0.8. Furthermore, ECCE in Jiangsu Province fluctuated upward during this period, with clear regional clustering characteristics. The study’s conclusions provide information about how carbon emissions from small-scale energy use are estimated. They also serve as a foundation for the creation of regional energy conservation and emission reduction policies, as well as a small-scale assessment of the present state.
Educational Poverty and Deprivation: Attitudes and the Role of Teachers during the Pandemic
Authors: Colombo Maddalena , Mesa Diego , Battilocchi Gianluca , Truscello Gianluca
Published: September 2024

Educational poverty is widespread in compulsory education in terms of poor learning and socio-cultural disadvantages; it affects students with disabilities, students with learning difficulties, and those with an immigrant background. During the pandemic crisis, students with special educational needs (SENs) mostly suffered the risk of an additional deprivation. Within an advanced inclusive model during school closure, the Italian State guaranteed access to schools only to students with disabilities. What effects did this “equalizing” measure produce? What kinds of attitudes have teachers adopted and what roles have they played (active vs. passive) in order to make it sustainable? What was their acknowledgement of the educational poverty and deprivation? To what extent were their attitudes differentiated? This article explores the roles and attitudes of teachers during periods of lockdown as a result of the COVID-19 pandemic in Italy. We assume that the teachers’ attitudes were differentiated on the basis of five cultural dimensions (auto/hetero-referencing; vision of remote teaching; relationship with bureaucracy, relationships with students; teaching as performance or relation), and two functions (classroom teachers/ SENs teachers). The study is based on 42 semi-structured interviews conducted with teachers, principals, and parents in six lower secondary public schools with high rates of SENs students and multi-ethnicity (Northern Italy), and focused on the organisational, teaching, and relational aspects. Results seem to confirm that not all teachers agreed with the Ministry line and adopted a range of responses to the challenge of maintaining inclusivity, from the inert/impatient to the creative/active ones. Their attitudes changed during the post-pandemic period from atomistic to more collaborative, and their preoccupation with the fragile “uncertified students” increased. The article closes with recommendations on teacher training.
Change in Land Use Affects Soil Organic Carbon Dynamics and Distribution in Tropical Systems
Authors: Saravia-Maldonado Selvin Antonio , Rodríguez-González María Ángeles , Ramírez-Rosario Beatriz , Fernández-Pozo Luis Francisco
Published: September 2024

Anthropogenic land cover change is directly responsible for the deforestation and degradation of tropical forests. In this context, assessing soil organic carbon (SOC) stocks is key to understanding the impact of anthropogenic activities on SOC so that we can implement management practices that effectively reduce emissions or promote carbon sequestration. Our objective was to assess the effect of land-use change on the dynamics and distribution of SOC in three systems (agriculture, pasture and agroforestry) after 40 years of deforestation in a tropical dry forest in the central–eastern region of Honduras. For this purpose, the bulk density, percentage of coarse fragments (>2 mm) and soil organic carbon content were determined at three depths (0.00–0.10 m, 0.10–0.20 m and 0.20–0.30 m). The results showed an increase in bulk density for all new uses, although soil compaction had not yet occurred. In terms of total soil organic carbon (TOC) stocks, deforestation caused a decrease from 17% to 48% in agricultural and agroforestry soils, respectively; on the other hand, grasslands did not show significant differences compared to tropical dry forest, suggesting that they have a high potential as carbon sinks in deforested tropical areas. However, this did not imply a better state of the system, as the greatest increases in bulk density were found in pastures.
Validation of Saliva as the Clinical Specimen Type for a University-Wide COVID-19 Surveillance Program
Authors: Farrell Michael L. , Bryksin Anton V. , Ryan Emily , Lin Jessica , Djeddar Naima , Khunteev German , Holton Benjamin , Paca Miles , Speller Nicholas , Merrill James T. , Ross Ted M. , Hogan Robert J. , Gibson Greg , García Andrés J. , Shannon Michael P.
Published: September 2024

At the beginning of the COVID-19 pandemic, the Georgia Institute of Technology made the decision to keep the university doors open for on-campus attendance. To manage COVID-19 infection rates, internal resources were applied to develop and implement a mass asymptomatic surveillance program. The objective was to identify infections early for proper follow-on verification testing, contact tracing, and quarantine/isolation as needed. Program success depended on frequent and voluntary sample collection from over 40,000 students, faculty, and staff personnel. At that time, the nasopharyngeal (NP) swab, not saliva, was the main accepted sample type for COVID-19 testing. However, due to collection discomfort and the inability to be self-collected, the NP swab was not feasible for voluntary and frequent self-collection. Therefore, saliva was selected as the clinical sample type and validated. A saliva collection kit and a sample processing and analysis workflow were developed. The results of a clinical sample-type comparison study between co-collected and matched NP swabs and saliva samples showed 96.7% positive agreement and 100% negative agreement. During the Fall 2020 and Spring 2021 semesters, 319,988 samples were collected and tested. The program resulted in maintaining a low overall mean positivity rate of 0.78% and 0.54% for the Fall 2020 and Spring 2021 semesters, respectively. For this high-throughput asymptomatic COVID-19 screening application, saliva was an exceptionally good sample type.
Combining Short- and Long-Read Sequencing Technologies to Identify SARS-CoV-2 Variants in Wastewater
Authors: Jayme Gabrielle , Liu Ju-Ling , Galvez Jose Hector , Reiling Sarah Julia , Celikkol Sukriye , N’Guessan Arnaud , Lee Sally , Chen Shu-Huang , Tsitouras Alexandra , Sanchez-Quete Fernando , Maere Thomas , Goitom Eyerusalem , Hachad Mounia , Mercier Elisabeth , Loeb Stephanie Katharine , Vanrolleghem Peter A. , Dorner Sarah , Delatolla Robert , Shapiro B. Jesse , Frigon Dominic , Ragoussis Jiannis , Snutch Terrance P.
Published: September 2024

During the COVID-19 pandemic, the monitoring of SARS-CoV-2 RNA in wastewater was used to track the evolution and emergence of variant lineages and gauge infection levels in the community, informing appropriate public health responses without relying solely on clinical testing. As more sublineages were discovered, it increased the difficulty in identifying distinct variants in a mixed population sample, particularly those without a known lineage. Here, we compare the sequencing technology from Illumina and from Oxford Nanopore Technologies, in order to determine their efficacy at detecting variants of differing abundance, using 248 wastewater samples from various Quebec and Ontario cities. Our study used two analytical approaches to identify the main variants in the samples: the presence of signature and marker mutations and the co-occurrence of signature mutations within the same amplicon. We observed that each sequencing method detected certain variants at different frequencies as each method preferentially detects mutations of distinct variants. Illumina sequencing detected more mutations with a predominant lineage that is in low abundance across the population or unknown for that time period, while Nanopore sequencing had a higher detection rate of mutations that are predominantly found in the high abundance B.1.1.7 (Alpha) lineage as well as a higher sequencing rate of co-occurring mutations in the same amplicon. We present a workflow that integrates short-read and long-read sequencing to improve the detection of SARS-CoV-2 variant lineages in mixed population samples, such as wastewater.
A Quadruple Gene-Deleted Live BoHV-1 Subunit RVFV Vaccine Vector Reactivates from Latency and Replicates in the TG Neurons of Calves but Is Not Transported to and Shed from Nasal Mucosa
Authors: Pavulraj Selvaraj , Stout Rhett W. , Paulsen Daniel B. , Chowdhury Shafiqul I.
Published: September 2024

Bovine herpesvirus type 1 (BoHV-1) establishes lifelong latency in trigeminal ganglionic (TG) neurons following intranasal and ocular infection in cattle. Periodically, the latent virus reactivates in the TG due to stress and is transported anterogradely to nerve endings in the nasal epithelium, where the virus replicates and sheds. Consequently, BoHV-1 is transmitted to susceptible animals and maintained in the cattle population. Modified live BoHV-1 vaccine strains (BoHV-1 MLV) also have a similar latency reactivation. Therefore, they circulate and are maintained in cattle herds. Additionally, they can regain virulence and cause vaccine outbreaks because they mutate and recombine with other circulating field wild-type (wt) strains. Recently, we constructed a BoHV-1 quadruple mutant virus (BoHV-1qmv) that lacks immune evasive properties due to UL49.5 and glycoprotein G (gG) deletions. In addition, it also lacks the gE cytoplasmic tail (gE CT) and Us9 gene sequences designed to make it safe, increase its vaccine efficacy against BoHV-1, and restrict its anterograde neuronal transport noted above. Further, we engineered the BoHV-1qmv-vector to serve as a subunit vaccine against the Rift Valley fever virus (BoHV-1qmv Sub-RVFV) (doi: 10.3390/v15112183). In this study, we determined the latency reactivation and nasal virus shedding properties of BoHV-1qmv (vector) and BoHV-1qmv-vectored subunit RVFV (BoHV-1qmv sub-RVFV) vaccine virus in calves in comparison to the BoHV-1 wild-type (wt) following intranasal inoculation. The real-time PCR results showed that BoHV-1 wt- but not the BoHV-1qmv vector- and BoHV-1qmv Sub-RVFV-inoculated calves shed virus in the nose following dexamethasone-induced latency reactivation; however, like the BoHV-1 wt, both the BoHV-1qmv vector and BoHV-1qmv Sub-RVFV viruses established latency, were reactivated, and replicated in the TG neurons. These results are consistent with the anterograde neurotransport function of the gE CT and Us9 sequences, which are deleted in the BoHV-1qmv and BoHV-1qmv Sub-RVFV.
Unleashing Nature’s Allies: Comparing the Vertical Transmission Dynamics of Insect-Specific and Vertebrate-Infecting Flaviviruses in Mosquitoes
Authors: Peterson Alyssa J. , Hall Roy A. , Harrison Jessica J. , Hobson-Peters Jody , Hugo Leon E.
Published: September 2024

Insect-specific viruses (ISVs) include viruses that are restricted to the infection of mosquitoes and are spread mostly through transovarial transmission. Despite using a distinct mode of transmission, ISVs are often phylogenetically related to arthropod-borne viruses (arboviruses) that are responsible for human diseases and able to infect both mosquitoes and vertebrates. ISVs can also induce a phenomenon called “superinfection exclusion”, whereby a primary ISV infection in an insect inhibits subsequent viral infections of the insect. This has sparked interest in the use of ISVs for the control of pathogenic arboviruses transmitted by mosquitoes. In particular, insect-specific flaviviruses (ISFs) have been shown to inhibit infection of vertebrate-infecting flaviviruses (VIFs) both in vitro and in vivo. This has shown potential as a new and ecologically friendly biological approach to the control of arboviral disease. For this intervention to have lasting impacts for biological control, it is imperative that ISFs are maintained in mosquito populations with high rates of vertical transmission. Therefore, these strategies will need to optimise vertical transmission of ISFs in order to establish persistently infected mosquito lines for sustainable arbovirus control. This review compares recent observations of vertical transmission of arboviral and insect-specific flaviviruses and potential determinants of transovarial transmission rates to understand how the vertical transmission of ISFs may be optimised for effective arboviral control.
N-Acetylcysteine Inhibits Coxsackievirus B3 Replication by Downregulating Eukaryotic Translation Elongation Factor 1 Alpha 1
Authors: Wang Yao , Luan Tian , Wang Lixin , Feng Danxiang , Dong Yanyan , Li Siwei , Yang Hong , Chen Yang , Fei Yanru , Lin Lexun , Pan Jiahui , Zhong Zhaohua , Zhao Wenran
Published: September 2024

Group B Coxsackieviruses (CVB) are one of the causative pathogens of myocarditis, which may progress to cardiomyopathy. The pathogenesis of CVB is not fully understood, and effective antiviral therapy is not available. N-acetylcysteine (NAC), the classic antioxidant, has been used in clinical practice for several decades to treat various medical conditions. In this study, the anti-CVB effect of NAC was investigated. We show that NAC dramatically suppressed viral replication and alleviated cardiac injury induced by CVB3. To further study the antiviral mechanism of NAC, RNA-sequencing was performed for CVB3-infected cells with NAC treatment. We found that eukaryotic elongation factor 1 alpha 1 (EEF1A1) is one of the most upregulated genes in CVB3-infected cells. However, EEF1A2, the highly homologous isoform of EEF1A1, remains unchanged. EEF1A1 expression was significantly suppressed by NAC treatment in CVB3-infected cells, while EEF1A2 was not affected. eEF1A1 knockdown significantly inhibited CVB3 replication, implicating that eEF1A1 facilitates viral replication. Importantly, we show that eEF1A1, which was not expressed in the myocardia of newborn mice, was significantly upregulated by CVB3 infection. NAC markedly downregulated the expression of eEF1A1 but not eEF1A2 in the myocardia of CVB3-infected mice. Furthermore, NAC accelerated eEF1A1 degradation by promoting autophagy in CVB3-infected cells. We show that p62, one of the critical adaptors of autophagic targets, interacts with eEF1A1 and was downregulated in CVB3-infected cells upon NAC treatment. Taken together, this study demonstrated that NAC shows a potent anti-CVB effect through the downregulation of eEF1A1.