bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt
Authors: Mahmoud Hassan Y. A. H. , Ali Alsagher O. , Tanaka Tetsuya
Published: May 2023

Anaplasmosis is a severe tickborne disease of ruminants caused by Anaplasma marginale. A. marginale is distributed worldwide and attacks erythrocytes, resulting in an increased body temperature, anemia, jaundice, abortion, and, in some cases, death. Animals infected with this pathogen become lifelong carriers. In this study, we aimed to detect and characterize A. marginale isolated from cattle, buffalo, and camel populations using novel molecular techniques in southern Egypt. In total, 250 samples (from 100 cattle, 75 water buffaloes, and 75 camels) were analyzed by PCR for the presence of Anaplasmataceae, specifically A. marginale. The animals varied in breed, age, and gender, with most showing no signs of severe disease. By species, A. marginale was found in 61 out of 100 (61%) cattle, 9 out of 75 (12%) buffaloes, and only 5 out of 75 (6.66%) camels. All A. marginale-positive samples were examined for the heat-shock protein groEL gene and, additionally, for major surface protein 4 (msp4) and major surface protein 5 (msp5) genes to enhance specificity. Phylogenetic analysis of A. marginale targeted three genes (groEL, msp4, and msp5). This study provides the first report on using three genes for A. marginale detection in Camelus dromedarius in southern Egypt and generated new phylogenetic data for A. marginale infections in camels. A. marginale infection is endemic in different animal species in southern Egypt. Screening herds for A. marginale is recommended even when the signs of anaplasmosis are absent.
Assessment of thermal changes in water buffalo mobilized from the paddock and transported by short journeys
Authors: Rodríguez-González Daniela , Guerrero Legarreta Isabel , Cruz-Monterrosa Rosy G. , Napolitano Fabio , Titto Cristiane Gonçalves , Abd El-Aziz Ayman H. , Hernández-Avalos Ismael , Casas-Alvarado Alejandro , Domínguez-Oliva Adriana , Mota-Rojas Daniel
Published: May 2023

Evaluating the welfare of buffaloes during transport is key to obtaining and commercializing high-quality meat products; however, effective assessments require recognizing several stressors that activate physiological mechanisms that can have repercussions on the health and productive performance of species. The aim of this study was to evaluate the surface temperatures of different body and head regions in this species during events prior, and posterior, to transport for short periods; that is, from paddock to loading. The second goal was to determine the level of correlation between thermal windows. This study used infrared thermography (IRT) to evaluate the surface temperature of 624 water buffaloes (Buffalypso breed) during 12 short trips (average duration = 2 h ± 20 min) by focusing on 11 regions of the body (Regio corporis), in the head regions (Regiones capitis) the face regions (Regiones faciei), Orbital region (Regio orbitalis) with special attention to structures such as the lacrimal caruncle, periocular area and lower eyelid (Regio palpebralis inferior); nasal region (Regio nasalis) with special attention to nostril thermal window; and regions of the skull (Regiones cranii) such as auricular region (Regio auricularis) with special attention to auditory canal and frontal–parietal region (Regio frontalis-parietalis) and trunk region (Truncus regionis) such as thoracic and abdominal regions, regions of the vertebral column (Columna vertebralis) with the thoracic vertebral region (Regio vertebralis thoracis) and lumbar region (Regio lumbalis); and regions of the pelvis limb (Regiones membri pelvini). Recordings were made during seven phases: paddock (P1), herding (P2), corral (P3), chute handling (P4), shipping (P5), pre- (P6), and post-transport (P7). A total of 48,048 readings were obtained from 11 thermal windows. The results showed that the surface temperatures of the windows increased by as much as 5°C during P2, P3, P5, P6, and P7 compared to P1 and P4 (p < 0.0001). Differences of at least 1°C were also observed between thermal windows in the craniofacial, lateral corporal, and peripheral zones (p < 0.0001). Finally, a strong positive correlation (r = 0.9,p < 0.0001) was found between the thermal windows. These findings lead to the conclusion that the surface temperature of the craniofacial and corporal regions of buffaloes transported for short periods varied in relation to the phase of mobilization (from paddock to post-transport), likely as a response to stressful factors, since herding and loading increased the thermal values in each window. The second conclusion is that there are strong positive correlations between central and peripheral thermal windows.
Key Factors That Influence the Frequency Range of Measured Leak Noise in Buried Plastic Water Pipes: Theory and Experiment
Authors: Scussel Oscar , Brennan Michael J. , de Almeida Fabrício Cézar L. , Iwanaga Mauricio K. , Muggleton Jennifer M. , Joseph Phillip F. , Gao Yan
Published: May 2023

The frequency range of the leak noise in buried water pipes, measured using acoustic correlators, depends significantly on the type of pipe and its location as well as the type of sensors used. Having a rough idea of this frequency range can be beneficial for operators prior to conducting tests; however, there is currently no method of predicting it except through practical experience, and no model-based approach yet exists. This issue is addressed in the present paper by using a concise and relatively simple analytical model of the water-pipe–soil system combined with the sensors’ frequency response. The influence of the various physical parameters of the system, such as the pipe and soil properties and the sensor type, on the cross-power spectral density (CPSD) of leak noise signals and, furthermore, the frequency range are investigated. The main factors that affect the bandwidth are the distance between the sensors, wave speed of the predominantly fluid-borne wave in the pipe and the attenuation of this wave. It is shown that the external medium has a profound effect on the propagation and, in turn, on the bandwidth. The approach to predicting this bandwidth is validated using experimental data from three different test sites.
Dynamic Model of a Novel Planar Cable Driven Parallel Robot with a Single Cable Loop
Authors: González-Rodríguez Antonio , Martín-Parra Andrea , Juárez-Pérez Sergio , Rodríguez-Rosa David , Moya-Fernández Francisco , Castillo-García Fernando J. , Rosado-Linares Jesús
Published: May 2023

Cable-Driven Parallel Robots (CDPRs) are a special kind of parallel manipulator that uses cables to control the position and orientation of the mobile platform or end effector. The use of cables instead of rigid links offers some advantages over their conventional rigid counterparts. As cables can only pull but not push, the number of cables (n) required to command the end-effector is always n+1. This configuration is known as fully-constrained, and it is the most extended configuration for CDPRs. Although CDPRs have many advantages, such as their ability to cover large working areas, one of their main problems is that their working area (workspace) is limited in comparison to its frame area (planar case) or frame volume (spatial case), due to the minimum and maximum allowed tensions. Depending on these tension values, the workspace can notoriously decrease. In order to tackle this problem, lots of works focus on solving kinematics or dynamics problems for cable sagging, i.e., they take into account sagging when modelling the robot kinematic and include these poses inside the usable robot workspace. Taking into account phenomena such as this increases the mathematical complexity of the problem, and much more complex techniques are required. On the other hand, the lack of workspace problem can be tackled by adding active or passive elements to the robot design. In this sense, this paper proposes two mechanical modifications: to add passive carriages to the robot frame and to use a single cable loop to command the end-effector position and orientation. This work presents the kinematic, static, and dynamic models of the novel design and shows the gain of workspace for a planar case while taking into account different parameters of the robot.
Investigating the Temperature Shock of a Plate in the Framework of a Static Two-Dimensional Formulation of the Thermoelasticity Problem
Authors: Sedelnikov Andry , Serdakova Valeria , Orlov Denis , Nikolaeva Alexandra
Published: May 2023

The paper investigates the stress–strain state of a homogeneous rectangular plate after a temperature shock. It is believed that the plate is the first approximation of the solar panel model of a small spacecraft. To study the stress–strain state of the plate, a two-dimensional thermoelasticity problem is posed. The problem has a static formulation, since it does not take into account the dynamics of the plate’s natural oscillations. These oscillations affect the stress–strain state through the initial deflection of the plate at the time of the temperature shock. This deflection changes the parameters of the temperature shock and does not allow the use of a one-dimensional formulation of the thermoelasticity problem. As a result of solving the static two-dimensional thermoelasticity problem, approximate solutions are obtained for the components of the plate point’s displacement vector after the temperature shock. An approximation of the temperature field is presented. A numerical simulation is carried out. The correspondence of the obtained approximate analytical dependencies of the components of the plate point’s displacement vector to the numerical simulation data is analyzed. The proposed method can be used to assess the significance of the influence of the small spacecraft’s solar panels temperature shock on the dynamics of its rotational motion.
A New Mode of a Natural Convection Solar Greenhouse Dryer for Domestic Usage: Performance Assessment for Grape Drying
Authors: Tawfik M. A. , Oweda Khaled M. , Abd El-Wahab M. K. , Abd Allah W. E.
Published: May 2023

It is known that the natural convection (NC) solar drying process is a simple and cheap method for drying foodstuffs, but it is not preferable for common users in the case of drying high-moisture content agro-products due to the slow rate of drying. Meanwhile, the forced convection (FC) drying process is most appropriate for such products, but its economic feasibility may be affected due to high initial and maintenance costs. Therefore, the present study proposed a controlled natural convection (CNC) drying mode using a solar greenhouse dryer (SGD) for drying grapes with two types of cover materials, glass and Plexiglas, through intermittent operation with a PV system to save energy as a simple and inexpensive domestic dryer instead of the common forced convection SGD and the conventional natural convection SGD. The obtained results of the new CNC drying mode using a Plexiglas SGD showed a higher drying rate than the NC drying mode and are close to the FC drying mode using the same cover material. The initial moisture content of the grapes was reduced from 5.91 g water/g dry matter to the final moisture content of 0.15 g water/g dry matter within 12 h and 15 h for the CNC and NC drying modes, respectively, using the Plexiglas SGD. Moreover, the thermal drying efficiency for the two mentioned drying modes was 12.5 and 9.7%, respectively. The Page model was found to be the most appropriate model to predict the kinetics of the SGD in all drying modes, regardless of the cover type. The new CNC drying mode using the Plexiglas SGD achieved the lowest cost per kg of dried grapes (1.26 USD/kg), the highest total saved costs over the lifespan of the dryer (USD 245.46) and the shortest payback period (1.08 years) compared to the other two dryers, NC-SGD and FC-SGD. Generally, the CNC-SGD had good performance over the NC-SGD because it is not affected by the fluctuation in the volume, velocity and direction of the inlet ambient air/wind during drying grapes as a high-moisture content product without external heating sources or complicated parts. Thus, the proposed drying system has the advantage in terms of simplicity, cheapness and saving energy compared to FC-SGD.
A Novel YOLOv6 Object Detector for Monitoring Piling Behavior of Cage-Free Laying Hens
Authors: Bist Ramesh Bahadur , Subedi Sachin , Yang Xiao , Chai Lilong
Published: May 2023

Piling behavior (PB) is a common issue that causes negative impacts on the health, welfare, and productivity of the flock in poultry houses (e.g., cage-free layer, breeder, and broiler). Birds pile on top of each other, and the weight of the birds can cause physical injuries, such as bruising or suffocation, and may even result in death. In addition, PB can cause stress and anxiety in the birds, leading to reduced immune function and increased susceptibility to disease. Therefore, piling has been reported as one of the most concerning production issues in cage-free layer houses. Several strategies (e.g., adequate space, environmental enrichments, and genetic selection) have been proposed to prevent or mitigate PB in laying hens, but less scientific information is available to control it so far. The current study aimed to develop and test the performance of a novel deep-learning model for detecting PB and evaluate its effectiveness in four CF laying hen facilities. To achieve this goal, the study utilized different versions of the YOLOv6 models (e.g., YOLOv6t, YOLOv6n, YOLOv6s, YOLOv6m, YOLOv6l, and YOLOv6l relu). The objectives of this study were to develop a reliable and efficient tool for detecting PB in commercial egg-laying facilities based on deep learning and test the performance of new models in research cage-free facilities. The study used a dataset comprising 9000 images (e.g., 6300 for training, 1800 for validation, and 900 for testing). The results show that the YOLOv6l relu-PB models perform exceptionally well with high average recall (70.6%), mAP@0.50 (98.9%), and mAP@0.50:0.95 (63.7%) compared to other models. In addition, detection performance increases when the camera is placed close to the PB areas. Thus, the newly developed YOLOv6l relu-PB model demonstrated superior performance in detecting PB in the given dataset compared to other tested models.
Seed Germination of Two Hybrids Obtained via Cross-Pollination between Miscanthus sinensis × Miscanthus sacchariflorus
Authors: Patanè Cristina , Saita Alessandro , Cosentino Salvatore L. , Iurato Antonella , Testa Giorgio
Published: May 2023

To date, economically and energy-costly vegetative propagation using rhizomes and tissue culture are the only options for the cultivation of Miscanthus spp. Some genotypes of miscanthus produce fertile seeds, offering a valid alternative to vegetative propagation. A preliminary study has been conducted on the seeds of two hybrids of miscanthus obtained via interspecific cross-pollination between M. sacchariflorus and M. sinensis: ‘GRC14’ (maternal: M. sacchariflorus) and ‘GRC10B’ (maternal: M. sinensis). Seeds were assessed for germination traits in a laboratory (at 25 °C in the dark) just after panicle harvest, and during 1-year storage at room temperature or at 8 °C. In a second experiment, the effects of gibberellic acid (GA3) solution at different concentrations (0, 50, 100, 300, 500 ppm) on the germination of freshly matured seeds were assessed. Poor germination just after harvest (<30%) indicates the occurrence of a physiological dormancy. Indeed, two months later, germination rose up to 76.7% in ‘GRC14’ and 50.8%, in ‘GRC10B’, and peaked at 95.6% in ‘GRC14’ and at 78% in ‘GRC10B’, 6 months after harvest. After a total of 12 months, germination was significantly reduced in both hybrids (≈60%). Seeds stored at room temperature lost dormancy earlier than those stored at 8 °C. Overall, germination was significantly improved by GA3, but the extent of the GA effect was genotype-dependent. In conclusion, a low establishment rate may result from direct seeding when fresh seed is used in the field. In this case, the use of GA3 is a possible strategy to ameliorate the impact of dormancy on seed germination. In the case of delayed sowings in late winter–early spring, seeds stored at room temperature after harvest may better perform than those stored at 8 °C.
Watering Shapes a Robust and Stable Microbial Community under Fusarium Crown Rot Infection
Authors: Xu Runlai , Du Chenghang , Gao Yutian , Zhou Xiaohan , Ejaz Irsa , Guo Jieru , Chen Kunhu , Ma Jun , Zhang Yinghua , Wang Zhimin , Sun Zhencai
Published: May 2023

Wheat crown rot, caused by Fusarium pseudograminearum, is an emerging soil-borne fungal disease and causes serious damage in areas where water is scarce. However, the interactions between plant, microbiome, and pathogen under different watering regimes are rarely known. In our study, we designed three watering patterns, including the early-drought (DR1), late-drought (DR2), and well-watered (WAT) patterns, and sampled at heading and grain filling, to investigate the effect of different watering regimes on the microbial community and disease severity. These findings showed that well-watered pattern at grain filling decreased the disease index compared to other patterns, and the bacterial community in the WAT and DR2 at grain filling showed higher alpha diversity (rhizosphere and root) and more stable structures (root). For the microbial network, irrespective of compartments, bacterial networks in the WAT and DR2 were more complex and connected with a higher average degree and clustering coefficient than DR1 at both periods. Furthermore, several potential beneficial microbes as biomarkers were enriched under good water conditions, specifically during the heading of DR2 and grain filling of WAT, including operational taxonomic units (OTUs) affiliated with the taxa of Arenimonas, Sphingomonas, Pseudoxanthomonas, Devosia, Lysobacter, Chitinophagaceae, and Gaiellales in the rhizosphere and root. Overall, the microbiome reshaped by good moisture or avoiding early drought should be emphasized and further used in controlling Fp-caused wheat crown rot.
Integrated Pathogen Management in Stevia Using Anaerobic Soil Disinfestation Combined with Different Fungicide Programs in USA, Mexico, and Paraguay
Authors: Sanabria-Velazquez Andres D. , Enciso-Maldonado Guillermo A. , Maidana-Ojeda Marco , Diaz-Najera Jose F. , Ayvar-Serna Sergio , Thiessen Lindsey D. , Shew H. David
Published: May 2023

Stevia is a semi-perennial crop grown to obtain the diterpene glycosides in its leaves, which are processed to manufacture non-caloric sweeteners. Anaerobic soil disinfestation (ASD) and fungicide application were evaluated for the management of stevia stem rot (SSR) and Septoria leaf spot (SLS) in lab and field experiments. In 2019 and 2021, experiments using carbon sources for ASD were carried out in microplots at NCSU (Clayton, NC, USA). In 2020/21 and 2021/22 seasons, field experiments were conducted at CSAEGRO, Mexico (MX) and CEDIT, Paraguay (PY) using a 2 × 3 factorial design with two ASD treatments and three fungicide treatments. ASD treatments included soil amended with cornmeal (MX) or wheat bran (PY) at a rate of 20.2 Mg ha−1, molasses at 10.1 Mg ha−1, and non-amended controls. Fungicide applications included chemical (azoxystrobin), organic (pyroligneous acid, PA), and a non-treated control. ASD was effective in reducing sclerotia viability of Sclerotium rolfsii in laboratory assays (p < 0.0001) and microplot trials (p < 0.0001) in NC. During field trials, the viability of sclerotia was significantly reduced (p < 0.0001) in soils amended with cornmeal + molasses or wheat bran + molasses as carbon sources for ASD. While there was no significant effectiveness of ASD in reducing SLS in 2020 and 2021 or SSR in MX 2020 field trials (p = 0.83), it did exhibit efficacy on SSR in 2021 (p < 0.001). The application of fungicides was significantly effective in reducing SSR (p = 0.01) and SLS (p = 0.001), with azoxystrobin being the most consistent and PA not being statistically different from the control or azoxystrobin. The effects of ASD on fresh yield were inconsistent, exhibiting significant effects in Mexican fields in 2020 but not in 2021. During Paraguayan field trials, ASD only significantly interacted with fungicide applications in the dry yield in 2022. In the 2020/21 MX and 2020 PY field trials, fungicides were significantly effective in enhancing dry leaf yields, with azoxystrobin showing the highest consistency among treatments and PA variable control. In conclusion, utilizing ASD alongside organic fungicides can be a valuable tool for stevia farmers when the use of chemical fungicides is limited. Further research is required to enhance consistency and reduce the costs associated with these treatments under diverse edaphoclimatic conditions.
Hyphantria cunea (Drury) Showed a Stronger Oviposition Preference for Native Plants after Invading the Subtropical Region of China
Authors: Li Zikun , Yin Hao , Li Yue , Wang Yiping , Yu Wenxian , Feng Bojie , Zhang Shouke
Published: May 2023

Hyphantria cunea (Drury) (Lepidoptera: Erebidae) is an invasive alien species that is widely distributed in northern China. H. cunea now occurs for the first time in the subtropical areas of China. Despite the importance of identifying oviposition host plants to control the spread of H. cunea, it is not clear whether H. cunea has a new oviposition preference for plant hosts in the new habitat after invading the subtropical region. At the same time, whether the presence of new plant volatiles of new hosts in new habitats influences the oviposition host preference of H. cunea has not been studied. In this study, we investigated the oviposition host plant preferences of H. cunea in the subtropical region of China. In the presence of multiple potential host plants, we found, for the first time, that H. cunea preferred Carya illinoinensis, Morus alba, and Carya cathayensis for oviposition. Through the effects on plant volatiles and on the oviposition, ten volatile compounds with significant differences in relative abundance between five plants with different fitness levels were significantly correlated with the oviposition preference of females. Cis-Hex-3-en-1-ol, (E)-3-Hexen-1-ol, cis-3-Hexenyl acetate 1, and 3-Pentadiene,4-methyl, had a significant positive correlation with the adult oviposition preference. Our results provide an important research basis for the study of green prevention and control strategies of subtropical H. cunea in China.
Genetic Diversity and Structure Revealed by Genomic Microsatellite Markers of Mytilus unguiculatus in the Coast of China Sea
Authors: Wei Xuelian , Fu Zeqin , Li Jiji , Ye Yingying
Published: May 2023

The hard-shelled mussel Mytilus unguiculatus plays an important role in mussel aquaculture in China due to its characteristic and nutritive value. In this study, 10 microsatellite loci are used to study the genetic diversity and genetic structure of seven location populations of M. unguiculatus in coastal areas of China. The results of amplification and genotyping show that the observed heterozygosity (Ho) and the expected heterozygosity (He) are 0.61~0.71 and 0.72~0.83, respectively. M. unguiculatus has high genetic diversity. The inbreeding index (FIS) of M. unguiculatus is significantly positive (FIS: 0.14~0.19), indicating that inbreeding might exist within populations. The genetic structure of M. unguiculatus is weak within populations from the East China Sea All results showed that genetic differences existed between the Qingdao population from the Yellow Sea and other populations from the East China Sea. It does not detect a population bottleneck event or expansion event in the populations. The results from this study can be used to provide important insights in genetic management units and sustainable utilization of M. unguiculatus resources and provide a better understand of genetic structure of marine bivalve with similar planktonic larval stage in the China Sea.
Investigations of Fish Assemblages Using Two Methods in Three Terminal Reservoirs of the East Route of South-to-North Water Transfer Project, China
Authors: Yan Huiguo , Chen Sibao , Liu Xia , Cheng Zhenhao , Schmidt Bjorn Victor , He Wenping , Cheng Fei , Xie Songguang
Published: May 2023

The terminal reservoirs of water transfer projects directly supply water for domestic, agricultural, and industrial applications, and the water quality of these reservoirs produce crucial effects on the achievement of project targets. Typically, fish assemblages are monitored as indicators of reservoir water quality, and can also be regulated for its improvement. In the present study, we compared traditional fish landing (TFL) and environmental DNA (eDNA) metabarcoding methods for monitoring fish assemblages in three terminal reservoirs of the East Route of the South-to-North Water Transfer Project, China. Results of TFL and eDNA showed similar assemblage structures and patterns of diversity and spatial distribution with obvious differences in fish composition across three examined reservoirs. Demersal and small fish were dominant in all reservoirs. In addition, a strong association between water transfer distance and assemblages and distribution of non-native fish was found. Our findings highlight the necessity of the fish assemblage monitoring and managing for water quality and revealed the impact of water diversion distance on the structure of fish assemblages and dispersal of alien species along the water transfer project.
Epibiont Cohabitation in Freshwater Shrimp Neocaridina davidi with the Description of Two Species New to Science, Cladogonium kumaki sp. nov. and Monodiscus kumaki sp. nov., and Redescription of Scutariella japonica and Holtodrilus truncatus
Authors: Maciaszek Rafał , Świderek Wiesław , Prati Sebastian , Huang Chih-Yang , Karaban Kamil , Kaliszewicz Anita , Jabłońska Aleksandra
Published: May 2023

This contribution presents the occurrence of epibiotic species associated with Neocaridina davidi shrimp collected in the wild, aquaculture ponds, and aquaria. A total of 900 shrimp are imported from Taiwan, three-quarters of which host at least one of the recorded epibionts. Among those epibionts, two species new to science are discovered, Cladogonium kumaki sp. nov. and Monodiscus kumaki sp. nov., while the other two, Holtodrilus truncatus and Scutariella japonica, are redescribed. The largest number of epibionts is found in shrimp collected from aquaculture ponds and the lowest in individuals from aquaria. Epibiont occurrence differs across designated microhabitats. The epibionts may be introduced alongside their host outside their native range, and their presence may affect shrimp breeding rates. Thus, more control over them should be provided. Their spread can be limited by removal from the host during molting or manually, as well as by using interspecies interactions.
Identification of Key Genes Associated with Heat Stress in Rats by Weighted Gene Co-Expression Network Analysis
Authors: Zhang Fan , Dou Jinhuan , Zhao Xiuxin , Luo Hanpeng , Ma Longgang , Wang Lei , Wang Yachun
Published: May 2023

Heat stress has been a big challenge for animal survival and health due to global warming. However, the molecular processes driving heat stress response were unclear. In this study, we exposed the control group rats (n = 5) at 22 °C and the other three heat stress groups (five rats in each group) at 42 °C lasting 30, 60, and 120 min, separately. We performed RNA sequencing in the adrenal glands and liver and detected the levels of hormones related to heat stress in the adrenal gland, liver, and blood tissues. Weighted gene co-expression network analysis (WGCNA) was also performed. Results showed that rectal temperature and adrenal corticosterone levels were significantly negatively related to genes in the black module, which was significantly enriched in thermogenesis and RNA metabolism. The genes in the green-yellow module were strongly positively associated with rectal temperature and dopamine, norepinephrine, epinephrine, and corticosterone levels in the adrenal glands and were enriched in transcriptional regulatory activities under stress. Finally, 17 and 13 key genes in the black and green-yellow modules were identified, respectively, and shared common patterns of changes. Methyltransferase 3 (Mettl3), poly(ADP-ribose) polymerase 2 (Parp2), and zinc finger protein 36-like 1 (Zfp36l1) occupied pivotal positions in the protein–protein interaction network and were involved in a number of heat stress-related processes. Therefore, Parp2, Mettl3, and Zfp36l1 could be considered candidate genes for heat stress regulation. Our findings shed new light on the molecular processes underpinning heat stress.
Effects of Long-Term Cold Stress on Growth Performance, Behavior, Physiological Parameters, and Energy Metabolism in Growing Beef Cattle
Authors: Wang Siyuan , Li Qi , Peng Jianhao , Niu Huaxin
Published: May 2023

This study aimed to evaluate the effects of a long-term cold environment on growth performance, physiological behavior, biochemical blood indexes, and hormone levels in Simmental cattle. Thirty Simmental crossbred bulls (weight = 350 ± 17 kg, 13–14 months old) were selected for two trials at autumn suitable temperatures (A-ST) and winter cold temperatures (W-CT) (15 cattle per season). The results showed that compared with the A-ST group, dry matter intake (p < 0.05) and feed:gain (p < 0.01) of the W-CT group increased, while body weight (p < 0.01) and average daily gain (p < 0.01) significantly decreased. Long-term cold stress also increased lying time (p < 0.01), feeding time (p < 0.05), and pulse rate (p < 0.01) in the W-CT group, while the rumen volatile fatty acids content (p < 0.01) and apparent digestibility of nutrients (p < 0.05) were significantly decreased. In terms of blood indicators, long-term cold stress increased the concentrations of glucose, glucose metabolic enzymes, glucocorticoids, triiodothyronine, and tetraiodothyronine in the plasma of the W-CT group (p < 0.05), but the levels of triglycerides, β-hydroxybutyrate, propionate, insulin, and growth hormone were decreased (p < 0.01). In summary, long-term cold stress may inhibit the digestive function of Simmental cattle and enhance the body’s energy metabolism and stress hormone imbalance, ultimately damaging the normal growth and development of the body.
The Association between Prepartum Rumination Time, Activity and Dry Matter Intake and Subclinical Hypocalcemia and Hypomagnesemia in the First 3 Days Postpartum in Holstein Dairy Cows
Authors: Emam Mahmoud H. , Shepley Elise , Mahmoud Mourad M. , Ruch Megan , Elmaghawry Sobhy , Abdelrazik Wafaa , Abdelaal Ahmed M. , Crooker Brian A. , Caixeta Luciano S.
Published: May 2023

Changes in prepartum behaviors such as total daily rumination (TDR), total daily activity (TDA) and dry matter intake (DMI) have the potential to be used as early indicators for cows at risk for subclinical hypocalcemia (SCH) or hypomagnesemia (HYM) after calving. Our objective was to investigate associations between average daily rate of change in total daily rumination (ΔTDR), total daily activity (ΔTDA) and dry matter intake (ΔDMI) from −3 days prepartum to calving with SCH and HYM at D0 or D3 relative to calving. Prepartum TDR, TDA and DMI were measured in 64 Holstein dairy cows. Blood samples were taken at D0 and D3 post-calving for the measurement of total plasma Ca and Mg concentration. Linear regression models were used to analyze the association between ΔTDR, ΔTDA and ΔDMI and SCH and HYM at D0 and D3 relative to calving. Potential confounding variables were offered to the models and backwards selection was used to determine which covariates to retain. No significant differences in prepartum ΔTDR, ΔTDA or ΔDMI were found between cows with or without SCH and HYM at D0 and D3. Our results suggest that the change in TDR, TDA and DMI in the last 3 days prepartum are not effective predictors for cows that will have SCH or HYM in the first 3 days postpartum.
Are There Differences between Methods Used for the Objective Estimation of Boar Sperm Concentration and Motility?
Authors: Sevilla Francisco , Soler Carles , Araya-Zúñiga Ignacio , Barquero Vinicio , Roldan Eduardo R. S. , Valverde Anthony
Published: May 2023

Artificial insemination in the swine industry, as in other species, demands adequate semen handling and accurate evaluation for the preparation of seminal doses. Sperm concentration and motility estimates are part of the semen evaluation process and are considered important for maximizing the yield of doses for insemination. In this study, methods were examined for their accuracy in the estimation of boar sperm concentration and motility. Assessments of sperm concentration were carried out using iSperm®, ISAS® v1, Open CASA v2, and the Accuread® photometer. Analyses of sperm motility were performed with iSperm®, ISAS® v1, and Open CASA v2 systems. In this study, boar semen samples were collected from 10 healthy males from two genetic lines. There were no relevant differences between sire lines when sperm concentration was assessed. A Bayesian analysis was applied to the four methods used to assess sperm concentration to examine whether there are relevant differences between them. Results suggested differences in the four methods, with a probability of relevance (PR) of 0.86–1.00. The iSperm® method revealed higher concentration values within the highest posterior density region at 95% confidence interval (HPD95%) = 167.0, 224.2 M/mL, whereas Open CASA v2 showed the lowest values, with HPD95% = 99.3, 155.9 M/mL. The iSperm® demonstrated higher reliability in measuring sperm concentration compared to other methods or devices within the given range of confidence. ANOVAs revealed relevant differences in the three methods of motility estimation. Overall, differences in boar sperm concentration and motility estimates were found using various methods, but further studies are needed for better characterization of these differences.
First Study on Gyrodactylus (Monogenea: Gyrodactylidae) in Morocco, with Description of a New Species from Luciobarbus pallaryi and Luciobarbus ksibi (Actinopterygii: Cyprinidae)
Authors: Shigoley Miriam Isoyi , Rahmouni Imane , Louizi Halima , Pariselle Antoine , Vanhove Maarten P. M.
Published: May 2023

To date, 41 species of Gyrodactylus have been described from Africa. However, none of these have been reported in Morocco. After identifying and examining 738 cyprinid host specimens, 26 specimens belonging to Gyrodactylus were found to parasitize the gills of nine species of Luciobarbus, Carasobarbus, and Pterocapoeta. The current study provides new information about the presence of a new parasitic species in Morocco, the first to be characterized on a species level in the Maghreb region. It describes in detail 12 specimens of Gyrodactylus isolated from the gills of Luciobarbus pallaryi (Pellegrin, 1919) and Luciobarbus ksibi (Boulenger, 1905). Based on morphoanatomical observations, the characterization of the specimens collected indicates a species of Gyrodactylus that is new to science, described here as Gyrodactylus nyingiae n. sp. The new species is different from previously described gyrodactylids infecting African cyprinid hosts because it has a longer hamulus total length, a longer hamulus root, a downward projecting toe of the marginal hook, and a trapezium-shaped ventral bar membrane with a slightly striated median portion and small rounded anterolateral processes. This study increases the total number of Gyrodactylus spp. found in African cyprinids to four.
Guanidine Acetic Acid Alters Tissue Bound Amino Acid Profiles and Oxidative Status in Finishing Pigs
Authors: Cui Yiyan , Yu Miao , Li Zhenming , Song Min , Tian Zhimei , Deng Dun , Ma Xianyong
Published: May 2023

This study aims to investigate the effects of guanidine acetic acid (GAA) on carcass traits, plasma biochemical parameters, tissue antioxidant capacity, and tissue-bound amino acid contents in finishing pigs. Seventy-two 140-day-old (body weight 86.59 ± 1.16 kg) crossbred pigs (Duroc × Landrace × Large White) were randomly assigned into four treatments with six replicate pens and three pigs per pen, which were fed the basal diets supplemented with 0, 0.05%, 0.10%, or 0.15% GAA, respectively. The plasma glucose concentration decreased, and creatine kinase activity and levels of GAA and creatine increased with the dietary GAA concentration. GAA linearly improved creatine content in the longissimus thoracis muscle (LM) and heart. The activities of superoxide dismutase, total antioxidant capacity, and glutathione peroxidase increased linearly in tissue or/and plasma, while the contents of malondialdehyde and protein carbonyl decreased linearly. GAA improved the contents of multiple-bound amino acids (such as proline or isoleucine) in the myocardium and LM. In conclusion, GAA enhanced the plasma biochemical parameters, oxidative status, and bound amino acid profiles of the heart and LM in finishing pigs.
Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles
Authors: Viudes-de-Castro Maria Pilar , Marco Jimenez Francisco , Vicente José Salvador
Published: May 2023

Rabbit is a reflexively ovulating species. Accordingly, in the practice of artificial insemination (AI) ovulation must be induced via exogenous GnRH (Gonadotropin-Releasing Hormone) administration, which may be performed intramuscularly, subcutaneously, or intravaginally. Unfortunately, the bioavailability of the GnRH analogue when added to the extender is lower due to the proteolytic activity in the seminal plasma and the poor permeability of the vaginal mucosa. The aim of the study was to refine the practice of AI practice in rabbits by replacing parenteral GnRH analogue administration (subcutaneous, intravenous, or intramuscular injection) with intravaginal application, while reducing its concentration in the diluent. Extenders containing the buserelin acetate in chitosan–dextran sulphate and chitosan–alginate nanoparticles were designed and 356 females were inseminated. Reproductive performance of females inseminated with the two experimental extenders, receiving 4 μg of buserelin acetate intravaginally per doe, was compared with that in the control group, the does of which were inseminated with the extender without the GnRH analogue and induced to ovulate with 1 μg of buserelin acetate administered intramuscularly. The entrapment efficiency of the chitosan–dextran sulphate complex was higher than that of chitosan–alginate. However, females inseminated with both systems showed similar reproductive performance. We conclude that both nanoencapsulation systems are an efficient way of intravaginal ovulation induction, allowing a reduction in the level of the GnRH analogue normally used in seminal doses from 15–25 μg to 4 μg.
Evaluating Biofilm Inhibitory Potential in Fish Pathogen, Aeromonas hydrophila by Agricultural Waste Extracts and Assessment of Aerolysin Inhibitors Using In Silico Approach
Authors: Arumugam Manikandan , Manikandan Dinesh Babu , Marimuthu Sathish Kumar , Muthusamy Govarthanan , Kari Zulhisyam Abdul , Téllez-Isaías Guillermo , Ramasamy Thirumurugan
Published: May 2023

Aeromonas hydrophila, an opportunistic bacteria, causes several devastating diseases in humans and animals, particularly aquatic species. Antibiotics have been constrained by the rise of antibiotic resistance caused by drug overuse. Therefore, new strategies are required to prevent appropriate antibiotic inability from antibiotic-resistant strains. Aerolysin is essential for A. hydrophila pathogenesis and has been proposed as a potential target for inventing drugs with anti-virulence properties. It is a unique method of disease prevention in fish to block the quorum-sensing mechanism of A. hydrophila. In SEM analysis, the crude solvent extracts of both groundnut shells and black gram pods exhibited a reduction of aerolysin formation and biofilm matrix formation by blocking the QS in A. hydrophila. Morphological changes were identified in the extracts treated bacterial cells. Furthermore, in previous studies, 34 ligands were identified with potential antibacterial metabolites from agricultural wastes, groundnut shells, and black gram pods using a literature survey. Twelve potent metabolites showed interactions between aerolysin and metabolites during molecular docking analysis, in that H-Pyran-4-one-2,3 dihydro-3,5 dihydroxy-6-methyl (−5.3 kcal/mol) and 2-Hexyldecanoic acid (−5.2 kcal/mol) showed promising results with potential hydrogen bond interactions with aerolysin. These metabolites showed a better binding affinity with aerolysin for 100 ns in molecular simulation dynamics. These findings point to a novel strategy for developing drugs using metabolites from agricultural wastes that may be feasible pharmacological solutions for treating A. hydrophila infections for the betterment of aquaculture.
Phylogenetic Analysis of Escherichia coli Isolated from Australian Feedlot Cattle in Comparison to Pig Faecal and Poultry/Human Extraintestinal Isolates
Authors: Messele Yohannes E. , Trott Darren J. , Hasoon Mauida F. , Veltman Tania , McMeniman Joe P. , Kidd Stephen P. , Djordjevic Steven P. , Petrovski Kiro R. , Low Wai Y.
Published: May 2023

The similarity of commensal Escherichia coli isolated from healthy cattle to antimicrobial-resistant bacteria causing extraintestinal infections in humans is not fully understood. In this study, we used a bioinformatics approach based on whole genome sequencing data to determine the genetic characteristics and phylogenetic relationships among faecal Escherichia coli isolates from beef cattle (n = 37) from a single feedlot in comparison to previously analysed pig faecal (n = 45), poultry extraintestinal (n = 19), and human extraintestinal E. coli isolates (n = 40) from three previous Australian studies. Most beef cattle and pig isolates belonged to E. coli phylogroups A and B1, whereas most avian and human isolates belonged to B2 and D, although a single human extraintestinal isolate belonged to phylogenetic group A and sequence type (ST) 10. The most common E. coli sequence types (STs) included ST10 for beef cattle, ST361 for pig, ST117 for poultry, and ST73 for human isolates. Extended-spectrum and AmpC β-lactamase genes were identified in seven out of thirty-seven (18.9%) beef cattle isolates. The most common plasmid replicons identified were IncFIB (AP001918), followed by IncFII, Col156, and IncX1. The results confirm that feedlot cattle isolates examined in this study represent a reduced risk to human and environmental health with regard to being a source of antimicrobial-resistant E. coli of clinical importance.
Antibiotics Knowledge, Attitudes and Behaviours among the Population Living in Cyprus
Authors: Sullman Mark J. M. , Lajunen Timo J. , Baddal Buket , Apostolou Menelaos
Published: May 2023

This study investigated the knowledge, attitudes and behaviours towards antibiotics among the general public living in the Republic of Cyprus (RoC) and the Turkish Republic of Northern Cyprus (TRNC) by using an online questionnaire. Differences were examined using independent samples t-tests, chi-square tests, Mann–Whitney U tests and Spearman’s rho. In total, 519 individuals completed the survey (RoC = 267, TRNC = 252), with an average age of 32.7, and 52.2% were female. Most citizens correctly identified paracetamol (TRNC = 93.7%, RoC = 53.9%) and ibuprofen (TRNC = 70.2%, RoC = 47.6%) as non-antibiotic medications. A substantial proportion thought antibiotics could treat viral infections, such as a cold (TRNC = 16.3%, RoC = 40.8%) or the flu (TRNC = 21.4%, RoC = 50.4%). Most participants understood that bacteria can become resistant to antibiotics (TRNC = 71.4%, RoC = 64.4%), that unnecessary use can lead to drug ineffectiveness (TRNC = 86.1%, RoC = 72.3%) and that they should always complete the course of antibiotics (TRNC = 85.7%, RoC = 64.0%). Positive attitudes towards antibiotics correlated negatively with knowledge in both samples, indicating that the more people know, the less positive their attitudes towards their use. The RoC appears to have tighter controls of over-the-counter (OTC) sales of antibiotics than TRNC. This study reveals that different communities can have varying levels of knowledge, attitudes and perceptions about antibiotic use. Tighter enforcement of the OTC regulations, educational efforts and media campaigns are needed for enhancing prudent antibiotic use on the island.
Untargeted Metabolomics for Unraveling the Metabolic Changes in Planktonic and Sessile Cells of Salmonella Enteritidis ATCC 13076 after Treatment with Lippia origanoides Essential Oil
Authors: Guillín Yuliany , Cáceres Marlon , Stashenko Elena E. , Hidalgo William , Ortiz Claudia
Published: May 2023

Nontyphoidal Salmonella species are one of the main bacterial causes of foodborne diseases, causing a public health problem. In addition, the ability to form biofilms, multiresistance to traditional drugs, and the absence of effective therapies against these microorganisms are some of the principal reasons for the increase in bacterial diseases. In this study, the anti-biofilm activity of twenty essential oils (EOs) on Salmonella enterica serovar Enteritidis ATCC 13076 was evaluated, as well as the metabolic changes caused by Lippia origanoides thymol chemotype EO (LOT-II) on planktonic and sessile cells. The anti-biofilm effect was evaluated by the crystal violet staining method, and cell viability was evaluated through the XTT method. The effect of EOs was observed by scanning electron microscopy (SEM) analysis. Untargeted metabolomics analyses were conducted to determine the effect of LOT-II EO on the cellular metabolome. LOT-II EO inhibited S. Enteritidis biofilm formation by more than 60%, without decreasing metabolic activity. Metabolic profile analysis identified changes in the modulation of metabolites in planktonic and sessile cells after LOT-II EO treatment. These changes showed alterations in different metabolic pathways, mainly in central carbon metabolism and nucleotide and amino acid metabolism. Finally, the possible mechanism of action of L. origanoides EO is proposed based on a metabolomics approach. Further studies are required to advance at the molecular level on the cellular targets affected by EOs, which are promising natural products for developing new therapeutic agents against Salmonella sp. strains.
Dietary Total Antioxidant Capacity, a Diet Quality Index Predicting Mortality Risk in US Adults: Evidence from the NIH-AARP Diet and Health Study
Authors: Ha Kyungho , Liao Linda M. , Sinha Rashmi , Chun Ock K.
Published: May 2023

Dietary total antioxidant capacity (TAC) is an index representing the total antioxidant power of antioxidants consumed via the diet. This study aimed to investigate the association between dietary TAC and mortality risk in the US adults using data from the NIH-AARP Diet and Health Study. A total of 468,733 adults aged 50–71 years were included. Dietary intake was assessed using a food frequency questionnaire. Dietary TAC from diet was calculated from antioxidants including vitamin C, vitamin E, carotenoids, and flavonoids, and TAC from dietary supplements was calculated from supplemental vitamin C, vitamin E, and beta-carotene. During a median follow-up of 23.1 years, 241,472 deaths were recorded. Dietary TAC was inversely associated with all-cause (hazard ratio (HR) for quintile 5 vs. quintile 1: 0.97, 95% confidence interval (CI): 0.96–0.99, p for trend < 0.0001) and cancer mortality (HR for quintile 5 vs. quintile 1: 0.93, 95% CI: 0.90–0.95, p for trend < 0.0001). However, dietary supplement TAC was inversely associated with cancer mortality risk only. These findings indicate that consuming a habitual diet high in antioxidants may reduce the risk of all-cause and cancer mortality and TAC from foods might confer greater health benefits than TAC from dietary supplements.