bluuebunny/crossref_metadata_2025 huggingface dataset:
Choriocapillaris Vascular Density Changes: Healthy vs. Advanced Exudative Age-Related Macular Degeneration Previously Treated with Multiple Anti-VEGF Intravitreal Injections
Authors: Savastano Maria Cristina , Rizzo Clara , Gambini Gloria , Savastano Alfonso , Falsini Benedetto , Bacherini Daniela , Caputo Carmela Grazia , Kilian Raphael , Faraldi Francesco , De Vico Umberto , Rizzo Stanislao
Published: October 2021

Purpose: To assess choriocapillaris vascular density (VD) in healthy and advanced exudative age-related macular degeneration (ae-AMD) patients by new full-range optical coherence tomography angiography (OCT-A). Method: In this observational, cross-sectional study, 21 healthy and 21 ae-AMD eyes, already treated with anti-VEGF, were enrolled. Angio-View retina patterns centered on fovea (6.4 × 6.4 mm) were acquired for all participants using Solix full-range OCT (Optovue Inc., Freemont, CA, USA). The main outcome was to compare choriocapillaris VD between healthy and ae-AMD eyes. Automated measurements of whole image choriocapillaris VD (%) and fovea grid-based (%) were collected for the analysis. Angio-View patterns were used to assess the flow area (mm2) of macular neovascularization (MNV) by contour flow measure algorithm. Best-corrected visual acuity (BCVA) of both groups was also used for the statistical analysis. Results: The mean age was 60.9 (±8.3) in healthy and 73.33 (±15.05) in ae-AMD eyes. The mean BCVA (ETDRS letters) was 98.47 (±1.50) in healthy and 7.04 (±5.96) in ae-AMD eyes. The Mann–Whitney test comparing choriocapillaries VD for whole and fovea healthy and ae-AMD eyes showed statistical significance (p < 0.0001 (t = 4.91; df = 40) and p < 0.0001 (t = 6.84; df = 40), respectively). Regarding, the correlation between MNV and VD of choriocapillaries, neither whole nor fovea areas were statistically significant (F = 0.38 (R2 = 0.01) and 1.68 (R2 = 0.08), respectively). Conclusions: Choriocapillaris VD showed a statistically significant reduction in comparison to healthy eyes in ae-AMD eyes. Choriocapillaris impairment can be seen in the early phase of MNV pathogenesis.
High School Students’ Performance Indicators in Distance Learning in Chemistry during the COVID-19 Pandemic
Authors: AlMahdawi Manal , Senghore Salieu , Ambrin Horia , Belbase Shashidhar
Published: October 2021

All private and public schools in the UAE had to run online classes as they closed their face-to-face classes due to the COVID-19 pandemic in the spring of 2021. In this context, the purpose of this study was to investigate the indicators of high school students’ performance in online chemistry classes in a private school in Al Ain, UAE. A quantitative study with an online survey questionnaire was carried out with 101 participants. The data were analyzed using One-Sample Wilcoxon Signed Ranked Test, Independent Sample Mann Whitney U, Independent Sample Kruskal Wallis H, and Spearman’s Rank Correlation in the Statistical Package for Social Sciences (IBM SPSS 26). The findings revealed that there was a statistically significant positive impact on critical thinking, collaborative skills, creativity and innovation, technology application, class participation, and overall achievement during online and distance learning of chemistry. There was a statistically significant difference in students’ critical thinking, collaborative skills, creativity and innovation, class participation, and achievement by gender and nationality. These skills were not statistically significantly different across students of grades 10, 11, and 12, except for creativity and innovation, which were significantly different between students of grades 11 and 12. All the six indicators of students’ performance had a significant correlation between each other, with the highest correlation between collaborative skills and participation level. These findings indicated that students’ performance in online chemistry classes during the COVID-19 pandemic provided opportunities to develop creativity and collaborative skills, together with better learning achievement as perceived by the students.
From Theory to Practice: An Adaptive Development of Design Education
Authors: Chiang I-Ying , Lin Po-Hsien , Kreifeldt John G. , Lin Rungtai
Published: October 2021

This study aims to discuss the adaptive challenge, and bridge the gap between theory and practice in design education. From now on, navigating design communities through the new era is a significant issue facing global competition and pluralistic society. First, this study reviews the essential evolution of design theories in the past few decades on man-machine system design, user-centered design, and user experience design. Second, based on three case studies of the REACH toothbrushes, an IEF wearable computer, and a LINNAK twin-cup, the research findings are offered to witness the advancement and transformation from hi-tech to hi-touch. Furthermore, this study summarizes three paradigms to interpret the adaptive evolution in design education. Finally, the authors propose three directions for the advancement of the creative industry and design education. The contributions of this study are to (1) clarify the interrelations between the theory and practice of design via the three foci of the human factors, human actors, and cultural aspects; (2) demonstrate the transformation of the archetypal model of user–tool–task employing illustrative paradigms; (3) identify the evolution of design education with contextual stages in the past decades; and (4) propose orientated perspectives for the personnel and institutes of the design industry and design education.
Advanced Authentication Method by Geometric Data Analysis Based on User Behavior and Biometrics for IoT Device with Touchscreen
Authors: Lee Jiwoo , Park Sohyeon , Kim Young-Gon , Lee Eun-Kyu , Jo Junghee
Published: October 2021

The Internet of Things (IoT) technology is rapidly being applied to real life, but the application of a corresponding secure and convenient authentication method is still in significant challenge. So far, pattern, password and fingerprint authentication are the most used methods, but it is important to address various security vulnerabilities and limitations of these approaches. In the case of fingerprint recognition, additional hardware such as a fingerprint scanner is required, which causes cost issues and could be vulnerable to fingerprint theft. To solve this problem, this paper proposes a model that uses both biometric and behavioral authentication at the same time. This method exploits the biometric authentication that measures the length of the contact region that occurs when three fingers are placed side by side on the touch screen or pad. In addition, it utilizes the behavioral authentication itself using three-finger L-shape touch, as well as secure geometric information generated by smart watch such as acceleration sensors. Therefore, this proposed model will be useful to implement more secure, rapid and user-friendly way of authentication in many practical busy and buzzling field where deal with sensitive private information.
Natural Convection over Two Superellipse Shapes with a Porous Cavity Populated by Nanofluid
Authors: Alsedais Noura
Published: October 2021

The influences of superellipse shapes on natural convection in a horizontally subdivided non-Darcy porous cavity populated by Cu-water nanofluid are inspected in this paper. The impacts of the inner geometries (n=0.5,1,1.5,4), Rayleigh number (103≤Ra≤106), Darcy number (10−5≤Da≤10−2), porosity (0.2≤ϵ≤0.8), and solid volume fraction (0.01≤∅≤0.05) on nanofluid heat transport and streamlines were examined. The hot superellipse shapes were placed in the cavity’s bottom and top, while the adiabatic boundaries on the flat walls of the cavity were considered. The governing equations were numerically solved using the finite volume method (FVM). It was found that the movement of the nanofluid upsurged as Ra boosted. The temperature distributions in the cavity’s core had an inverse relationship with increasing Rayleigh number. An extra porous resistance at lower Darcy numbers limited the nanofluid’s movement within the porous layers. The mean Nusselt number decreased as the porous resistance increased (Da≤10−4). The flow and temperature were strongly affected as the shape of the inner superellipse grew larger.
Austroboletus brunneisquamus (Boletaceae, Boletales), a New Ectomycorrhizal Fungus from a Tropical Rainforest, China
Authors: Xu Chang , Liang Zhi-Qun , Su Ming-Sheng , Jiang Shuai , Chen Yun , Fan Yu-Guang , Zeng Nian-Kai
Published: October 2021

Austroboletusbrunneisquamus (Boletaceae/Boletales), an ectomycorrhizal fungus, is described as a new species from a tropical rainforest in China based on morphological and molecular evidence. It is morphologically characterized by a subtomentose pileal surface when young, which cracks into areolae, having large, pale brown and brown to dark brown scales, a stipe with yellowish brown reticulation, basidiospores measuring (11–)12–14.5(–15) × 6–8(–8.5) μm, with fine cristate to subreticulate ornamentation, and a pileipellis in the form of a cutis. A detailed description, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented.
Genome-Wide Identification and Functional Analysis of the Basic Helix-Loop-Helix (bHLH) Transcription Family Reveals Candidate PtFBH Genes Involved in the Flowering Process of Populus trichocarpa
Authors: Ye Yang , Xin Haodong , Gu Xiting , Ma Jianwen , Li Lingli
Published: October 2021

As one of the largest TF families+ in plants, the basic helix-loop-helix (bHLH) family plays an important part in the growth and development of many plants. FLOWERING BHLH (FBH) encodes a bHLH-type transcriptional factor related to the flowering process. Poplar is a model woody plant as well as an important economic tree species with a small genome. However, the characteristics of the bHLHs and FBHs gene family in the newest version of Populustrichocarpa genome have not been analyzed yet. We identified 233 PtbHLHs and 10 PtFBHs in the newest version genome, and PtbHLHs were classified into 21 groups with FBH subfamily occupying one, supported by phylogenetic analysis, exon–intron patterns, and conserved protein motifs. These PtHLHs were distributed on 19 chromosomes unevenly and expressed in nucleus mainly. Gene duplication and synteny analysis have indicated that the PtbHLHs gene family has undergone strong purification selection during the evolution process. The cis-elements analysis has suggested that PtbHLHs may be related to the growth and development. Conserved residues of FBHs among Arabidopsis and poplar were also identified. Expression of 227 PtHLH genes (6 unmatched, 13 no expressed) showed diverse patterns in different tissues, implying their multiple functions. Protein–protein interaction network prediction and expression patterns in three states of the flowering process (Flowers-Dormant, Flowers-Expanding and Flowers-Expanded) suggested that some members of PtbHLH and PtFBH family may be involved in the flowering process. Our comprehensive and systematic analysis can provide some valuable clues and basic reference toward further investigations on physiological and molecular functions of PtbHLHs.
Determining the Contributions of Vegetation and Climate Change to Ecosystem WUE Variation over the Last Two Decades on the Loess Plateau, China
Authors: Chang Xiaoge , Wang Zhihui , Wei Fengyuan , Xiao Peiqing , Shen Zhenzhou , Lv Xizhi , Shi Yonglei
Published: October 2021

Exploring the variations in the water use efficiency (WUE) is helpful in gaining an in-depth understanding of the regional carbon and water cycles on the Chinese Loess Plateau (CLP). Here, we employed the spatial variations in the WUE and the quantitative contributions of the influencing factors, including the precipitation (P), temperature (Temp), vapor pressure deficit (VPD), sunshine duration (SD), and leaf area index (LAI), with the drought index varying over the last two decades. Results showed that the multiyear average WUE decreased significantly as the drought index increased for all of the vegetation types. Per-pixel interannual variability of WUE trend was 0.024 gC·m−2·mm−1·year−1. As the drought index increased, the WUE initially increased and then decreased for the forests, grassland, and shrubland, and their peaks occurred at drought index values of 2.60–3.10. Among the influencing factors, the WUE was predominantly controlled by the LAI, with an impact and relative contribution of 0.014 gC·m−2·mm−1·year−1 and 58.3%, respectively. The P and SD contributed the least to the trend in WUE, and impact and relative contribution of both were 0.001 gC·m−2·mm−1·year−1 and 4.17%. Our study also demonstrated that the LAI was the dominant factor affecting the WUE trends for grassland and the Yan River due to the structural parameters and geographical location. In addition, the impact and relative contribution of the residual factors on the WUE trend were 0.004 gC·m−2·mm−1·year−1 and 16.7%. Our findings suggested that comprehensive effects such as micro-geomorphic changes and nitrogen deposition could not be ignored except for vegetation and climate change. This study will clarify the spatial and temporal evolution of WUE and its influence mechanism.
Parameter Variability in Viscous Convection
Authors: Holzbecher Ekkehard
Published: October 2021

For the optimal design of cooling and heating devices, the properties of the included fluids are crucial. The temperature dependence of viscosity deserves attention, as changes can be one order of magnitude or more. Here we examine the influence on convective motions by simulating a heating and cooling experiment with a vertical cylinder by finite element computational fluid dynamics (CFD) models. Such an experimental setup in which flow patterns are determined by transient viscous convection has not been simulated before. Evaluating the general behavior of the experiment in 2D, we find a dynamic phase after and before phases with moderate changes. Flow patterns in the dynamic phase change significantly with the temperature range of the experiment. We compare the outcome of the numerical models with results from laboratory experiments, finding major discrepancies concerning the flow patterns in the dynamic phase. 3D modeling shows weaker dynamics but does not show good timing with the experiment. The study depicts the importance of parameter dependencies for convective motions and demonstrates the capabilities and limitations of models to reproduce details of viscous convection.
Glycemic Index Values of Pasta Products: An Overview
Authors: Di Pede Giuseppe , Dodi Rossella , Scarpa Cecilia , Brighenti Furio , Dall’Asta Margherita , Scazzina Francesca
Published: October 2021

Durum wheat pasta is considered a low-glycemic index (GI) food. In recent years, the interest in developing enriched pasta has increased. Since both the formulation and processing technologies may affect the GI, this study aimed to investigate the GI values of pasta products (pp) reported in the literature until 2020. GI values of pp analyzed following the ISO guidelines were included in this survey. A total of 95 pp were identified and, according to their formulation, classified into 10 categories (n, mean GI): category n 1: 100% refined wheat (35, 55); category n 2: 100% whole wheat (6, 52); category n 3: other cereal-based products (8, 52); category n 4: containing egg (5, 52); category n 5: gluten free (11, 60); category n 6: containing legumes (9, 46); category n 7: noodles and vermicelli (9, 56); category n 8: containing vegetable or algae (6, 51); category n 9: containing other ingredients (5, 37); category n 10: stuffed (1, 58). Overall, pasta is confirmed to be a medium–low-GI food, even if a high variability among or within each category emerged. The formulation of enriched pp able to elicit a controlled glycemic response could represent a strategy to improve the nutritional value of pasta.
Effect of Moisture Distribution Changes Induced by Different Cooking Temperature on Cooking Quality and Texture Properties of Noodles Made from Whole Tartary Buckwheat
Authors: Zou Shuping , Wang Lijuan , Wang Aili , Zhang Qian , Li Zaigui , Qiu Ju
Published: October 2021

While precooking and processing have improved the quality of gluten-free noodles, the effects of different cooking temperatures on their quality—neither gluten-free noodles nor whole Tartary buckwheat noodles—have rarely been clarified. This study investigated the key role of moisture distribution induced by different cooking temperatures in improving the noodle quality of whole Tartary buckwheat. The results showed that cooking temperatures higher than 70 °C led to a sharp increase in cooking loss, flavonoid loss and the rate of broken noodles, as well as a sharp decrease in water absorption. Moreover, the noodles cooked at 70 °C showed the lowest rate of hardness and chewiness and the highest tensile strength of all cooking temperatures from 20 °C to 110 °C. The main positive attribute of noodles cooked at 70 °C might be their high uniform moisture distribution during cooking. Cooking at 70 °C for 12 min was determined as the best condition for the quality improvement of whole Tartary buckwheat noodles. This is the first study to illustrate the importance of cooking temperatures on the quality of Tartary buckwheat noodles. More consideration must also be given to the optimal cooking conditions for different gluten-free noodles made from minor coarse cereals.
The Effect of Vacuum Deep Frying Technology and Raphanus sativus on the Quality of Surimi Cubes
Authors: Chen Jinghao , Lei Yi , Zuo Jiaxin , Guo Zebin , Miao Song , Zheng Baodong , Lu Xu
Published: October 2021

This study uses a response surface methodology to optimize the vacuum deep frying process of surimi cubes. The effects of vacuum deep frying temperature, frying time, and thickness on the hardness and color difference of surimi cubes with Raphanus sativus were studied. Further, the manuscript explored the quality changes of surimi cubes under different frying processes (vacuum deep frying, atmospheric deep frying, and shallow frying). Moreover, the Chinese Min-Cantonese cuisine-Raphanus sativus was utilized as auxiliary raw material to change the hardness and reduce the oil content. The optimal parameters of response surface methodology determined were: vacuum deep frying temperature 130 °C, frying time 900 s, and thickness 0.75 cm. Additionally, under this process condition, the hardness of the surimi chunks was 2015 ± 48.17 g, and the color difference was 23.27 ± 1.86. Surimi cubes without Raphanus sativus have superior elasticity and low hardness, and surimi cubes with Raphanus sativus have little color difference and high chewability. After the vacuum deep frying process, there was a high protein content and superior crispness. Shallow frying and adding Raphanus sativus effectively reduced the product’s oil content. Therefore, Raphanus sativus is suitable as a potential nutritional supplement to broaden its application in fried surimi foods.
Effect of Fermentation Parameters on Natto and Its Thrombolytic Property
Authors: Yang Yun , Lan Guangqun , Tian Xueyi , He Laping , Li Cuiqin , Zeng Xuefeng , Wang Xiao
Published: October 2021

Natto is a popular food because it contains a variety of active compounds, including nattokinase. Previously, we discovered that fermenting natto with the combination of Bacillus subtilis GUTU09 and Bifidobacterium animalis subsp. lactis BZ25 resulted in a dramatically better sensory and functional quality of natto. The current study further explored the effects of different fermentation parameters on the quality of natto fermented with Bacillus subtilis GUTU09 and Bifidobacterium BZ25, using Plackett–Burman design and response surface methodology. Fermentation temperature, time, and inoculation amount significantly affected the sensory and functional qualities of natto fermented with mixed bacteria. The optimal conditions were obtained as follows: soybean 50 g/250 mL, triangle container, 1% sucrose, Bacillus subtilis GUTU09 to Bifidobacterium BZ25 ratio of 1:1, inoculation 7%, fermentation temperature 35.5 °C, and fermentation time 24 h. Under these conditions, nattokinase activity, free amino nitrogen content, and sensory score were increased compared to those before optimization. They were 144.83 ± 2.66 FU/g, 7.02 ± 0.69 mg/Kg and 82.43 ± 5.40, respectively. The plate thrombolytic area and nattokinase activity both increased significantly as fermentation time was increased, indicating that the natto exhibited strong thrombolytic action. Hence, mixed-bacteria fermentation improves the taste, flavor, nattokinase activity, and thrombolysis of natto. This research set the groundwork for the ultimate manufacturing of natto with high nattokinase activity and free amino nitrogen content, as well as good sensory and thrombolytic properties.
Development of Gluten-Free Muffins with β-Glucan and Pomegranate Powder Using Response Surface Methodology
Authors: Kurek Marcin Andrzej , Moczkowska-Wyrwisz Małgorzata , Wyrwisz Jarosław , Karp Sabina
Published: October 2021

More consumers are being diagnosed with celiac disease or diseases in which wheat products should be avoided. For this reason, it is important to increase the range of gluten-free products available. In this study, it was decided to optimize the technology for the creation of a muffin with β-glucan (BG) and pomegranate (PG), while establishing water share (WT), using the response surface methodology. It was shown that β-glucan and water had the most significant influence on specific volume and moisture (p ≤ 0.001). However, the increase of hardness, color, and total phenolic content (TPC) was mainly influenced by the increase of pomegranate content (p ≤ 0.01 for harness and color and p ≤ 0.001 for TPC). Consumers accepted products high in β-glucan more than high in pomegranate. Optimization ended with a composition that included 1.89% BG, 9.51% PG, and 77.87% WT. There were no significant differences between the model and the experimental sample, apart from higher consumer acceptability.
3D Printed Chitosan-Pectin Hydrogels: From Rheological Characterization to Scaffold Development and Assessment
Authors: Zarandona Iratxe , Bengoechea Carlos , Álvarez-Castillo Estefanía , de la Caba Koro , Guerrero Antonio , Guerrero Pedro
Published: October 2021

Chitosan-pectin hydrogels were prepared, and their rheological properties were assessed in order to select the best system to develop scaffolds by 3D printing. Hydrogels showed a weak gel behavior with shear thinning flow properties, caused by the physical interactions formed between both polysaccharides, as observed by FTIR analysis. Since systems with high concentration of pectin showed aggregations, the system composed of 2 wt% chitosan and 2 wt% pectin (CHI2PEC2) was selected for 3D printing. 3D printed scaffolds showed good shape accuracy, and SEM and XRD analyses revealed a homogeneous and amorphous structure. Moreover, scaffolds were stable and kept their shape and size after a cycle of compression sweeps. Their integrity was also maintained after immersion in PBS at 37 °C, showing a high swelling capacity, suitable for exudate absorption in wound healing applications.
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon
Authors: Bitencourt Chaves Lana , Guimarães Glaucia de Oliveira , Perce-da-Silva Daiana de Souza , Banic Dalma Maria , Totino Paulo Renato Rivas , Machado Ricardo Luiz Dantas , Rodrigues-da-Silva Rodrigo Nunes , Pratt-Riccio Lilian Rose , Daniel-Ribeiro Cláudio Tadeu , Lima-Junior Josué da Costa
Published: October 2021

The Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for vivax malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the pvcyrpa genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the pvcyrpa gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct P. vivax endemic areas worldwide.
Genotype-Phenotype Correlations in PMM2-CDG
Authors: Vaes Laurien , Rymen Daisy , Cassiman David , Ligezka Anna , Vanhoutvin Nele , Quelhas Dulce , Morava Eva , Witters Peter
Published: October 2021

PMM2-CDG is a rare disease, causing hypoglycosylation of multiple proteins, hence preventing full functionality. So far, no direct genotype–phenotype correlations have been identified. We carried out a retrospective cohort study on 26 PMM2-CDG patients. We collected the identified genotype, as well as continuous variables indicating the disease severity (based on Nijmegen Pediatric CDG Rating Score or NPCRS) and dichotomous variables reflecting the patients’ phenotype. The phenotypic effects of patients’ genotype were studied using non-parametric and Chi-Square tests. Seventeen different pathogenic variants have been studied. Variants with zero enzyme activity had no significant impact on the Nijmegen score. Pathogenic variants involving the stabilization/folding domain have a significantly lower total NPCRS (p = 0.017): presence of the p.Cys241Ser mutation had a significantly lower subscore 1,3 and NPCRS (p = 0.04) and thus result in a less severe phenotype. On the other hand, variants involving the dimerization domain, p.Pro113Leu and p.Phe119Leu, resulted in a significantly higher NPCRS score (p = 0.002), which indicates a worse clinical course. These concepts give a better insight in the phenotypic prognosis of PMM2-CDG, according to their molecular base.
NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
Authors: Liang Tingming , Shen Lulu , Ji Yaya , Jia Lin , Dou Yuyang , Guo Li
Published: October 2021

Intrahepatic cholangiocarcinoma (ICC) is a common type of human cancer with a poor prognosis, and investigating the potential molecular mechanisms that can contribute to gene diagnosis and therapy. Herein, based on the recently concerned vertebrate-specific Cyr61/CTGF/NOV (CCN) gene family because of its important roles in diverse diseases, we obtained NOV/CCN3 to query for its potential roles in tumorigenesis via bioinformatics analysis. Experimental validations confirmed that both NOV mRNA and protein are up-regulated in two ICC cell lines, suggesting that it may promote cell migration and invasion by promoting EMT. To elucidate the detailed regulatory mechanism, miR-92a-3p is screened and identified as a negative regulatory small RNA targeting NOV, and further experimental validation demonstrates that miR-92a-3p contributes to NOV-mediated migration and invasion of ICC via the Notch signaling pathway. Our study reveals that NOV may be a potential target for diagnosing and treating ICC, which will provide experimental data and molecular theoretical foundation for cancer treatment, particularly for future precision medicine.
17q12 Recurrent Deletions and Duplications: Description of a Case Series with Neuropsychiatric Phenotype
Authors: Milone Roberta , Tancredi Raffaella , Cosenza Angela , Ferrari Anna Rita , Scalise Roberta , Cioni Giovanni , Battini Roberta
Published: October 2021

Syndromic neurodevelopmental disorders are usually investigated through genetics technologies, within which array comparative genomic hybridization (Array-CGH) is still considered the first-tier clinical diagnostic test. Among recurrent syndromic imbalances, 17q12 deletions and duplications are characterized by neurodevelopmental disorders associated with visceral developmental disorders, although expressive variability is common. Here we describe a case series of 12 patients with 17q12 chromosomal imbalances, in order to expand the phenotypic characterization of these recurrent syndromes whose diagnosis is often underestimated, especially if only mild traits are present. Gene content and genotype-phenotype correlations have been discussed, with special regard to neuropsychiatric features, whose impact often requires etiologic analysis.
Pairwise Correlation Analysis of the Alzheimer’s Disease Neuroimaging Initiative (ADNI) Dataset Reveals Significant Feature Correlation
Authors: Huckvale Erik D. , Hodgman Matthew W. , Greenwood Brianna B. , Stucki Devorah O. , Ward Katrisa M. , Ebbert Mark T. W. , Kauwe John S. K. ,   The Alzheimer’s Disease Neuroimaging Initiative,   The Alzheimer’s Disease Metabolomics Consortium, Miller Justin B.
Published: October 2021

The Alzheimer’s Disease Neuroimaging Initiative (ADNI) contains extensive patient measurements (e.g., magnetic resonance imaging [MRI], biometrics, RNA expression, etc.) from Alzheimer’s disease (AD) cases and controls that have recently been used by machine learning algorithms to evaluate AD onset and progression. While using a variety of biomarkers is essential to AD research, highly correlated input features can significantly decrease machine learning model generalizability and performance. Additionally, redundant features unnecessarily increase computational time and resources necessary to train predictive models. Therefore, we used 49,288 biomarkers and 793,600 extracted MRI features to assess feature correlation within the ADNI dataset to determine the extent to which this issue might impact large scale analyses using these data. We found that 93.457% of biomarkers, 92.549% of the gene expression values, and 100% of MRI features were strongly correlated with at least one other feature in ADNI based on our Bonferroni corrected α (p-value ≤ 1.40754 × 10−13). We provide a comprehensive mapping of all ADNI biomarkers to highly correlated features within the dataset. Additionally, we show that significant correlation within the ADNI dataset should be resolved before performing bulk data analyses, and we provide recommendations to address these issues. We anticipate that these recommendations and resources will help guide researchers utilizing the ADNI dataset to increase model performance and reduce the cost and complexity of their analyses.
Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
Authors: Gross Thomas , Becker Annette
Published: October 2021

Angiosperm flowers are the most complex organs that plants generate, and in their center, the gynoecium forms, assuring sexual reproduction. Gynoecium development requires tight regulation of developmental regulators across time and tissues. How simple on and off regulation of gene expression is achieved in plants was described previously, but molecular mechanisms generating complex expression patterns remain unclear. We use the gynoecium developmental regulator CRABS CLAW (CRC) to study factors contributing to its sophisticated expression pattern. We combine in silico promoter analyses, global TF-DNA interaction screens, and mutant analyses. We find that miRNA action, DNA methylation, and chromatin remodeling do not contribute substantially to CRC regulation. However, 119 TFs, including SEP3, ETT, CAL, FUL, NGA2, and JAG bind to the CRC promoter in yeast. These TFs finetune transcript abundance as homodimers by transcriptional activation. Interestingly, temporal–spatial aspects of expression regulation may be under the control of redundantly acting genes and require higher order complex formation at TF binding sites. Our work shows that endogenous regulation of complex expression pattern requires orchestrated transcription factor action on several conserved promotor sites covering almost 4 kb in length. Our results highlight the utility of comprehensive regulators screens directly linking transcriptional regulators with their targets.
Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells
Authors: Redon Christophe E. , Schmal Zoe , Tewary Gargi , Mangelinck Adèle , Courbeyrette Régis , Thuret Jean-Yves , Aladjem Mirit I. , Bonner William M. , Rübe Claudia E. , Mann Carl
Published: October 2021

H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the H2AFJ gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer.
Improving Adult Vaccination Status in the United States
Authors: Valdez R. Burciaga , Romero Korazon S.
Published: October 2021

Adult immunization practices leave much to be desired. Misinformation has increased mistrust. As a result, Latino and African American populations have low rates of annual flu vaccinations and, during the COVID-19 pandemic, lag behind for COVID-19 vaccination. Historically, healthcare staff have failed to adhere to adult immunization guidelines contributing to patient infections. Healthcare staff, both clinical and non-clinical, must lead by example by making “prevention primary”. Most adults may not realize they need immunizations. We recommend the following steps to increase immunization uptake: Make adult immunization a standard of patient care as we do for children. Assess immunization status at every clinical opportunity. Strongly recommend vaccinations needed. Administer needed vaccinations, multiple if warranted. Document vaccines received by your patient. Participate in your state’s immunization registry and work with community organizations that can help make adult immunization the norm.
How Can Hybrid Simulation Support Organizations in Assessing COVID-19 Containment Measures?
Authors: Cimini Chiara , Pezzotta Giuditta , Lagorio Alexandra , Pirola Fabiana , Cavalieri Sergio
Published: October 2021

Simulation models have always been an aid in epidemiology for understanding the spread of epidemics and evaluating their containment policies. This paper illustrates how hybrid simulation can support companies in assessing COVID-19 containment measures in indoor environments. In particular, a Hybrid Simulation (HS) is presented. The HS model consists of an Agent-Based Simulation (ABS) to simulate the virus contagion model and a Discrete Event Simulation (DES) model to simulate the interactions between flows of people in an indoor environment. Compared with previous works in the field of simulation and COVID-19, this study provides the possibility to model the specific behaviors of individuals moving in time and space and the proposed HS model could be adapted to several epidemiological conditions (just setting different parameters in the agent-based model) and different kinds of facilities. The HS approach has been developed and then successfully tested with a real case study related to a university campus in northern Italy. The case study highlights the potentials of hybrid simulation in assessing the effectiveness of the containment measures adopted during the period under examination in the pandemic context. From a managerial perspective, this study, exploiting the complementarity of the ABM and DES approaches in a HS model, provides a complete and usable tool to support decision-makers in evaluating different contagion containment measures.
Impact of Bariatric Surgery on the Healthy Eating Index, Binge Eating Behavior and Food Craving in a Middle Eastern Population: A Lebanese Experience
Authors: Jabbour Jana , Awada Dalia , Naim Nour , Al-Jawaldeh Ayoub , Haidar Ahmad Houssein , Mortada Hussein , Hoteit Maha
Published: October 2021

Even though bariatric surgeries (BS) are on the rise in Lebanon and the Middle East, the changes in diet quality, binge eating, and food cravings in this region are poorly studied peri-operatively. This cross-sectional study aimed to assess binge eating behaviors, food craving and the Healthy Eating Index (HEI) in Lebanese patients who underwent BS in a duration that exceeds 6 months. Evaluation included a dietary assessment of usual diet preoperatively and postoperatively. It included the collection of information on sociodemographic, anthropometric and surgical variables, as well as the administration of dietary recalls and questionnaires to calculate the HEI score, the Binge Eating Scale (BES) and the Food Craving Inventory (FCI). Participants (n = 60) were mostly females (85%) who had undergone sleeve gastrectomy (90%), with a mean duration since BS of 2.4 ± 1.8 years. Despite improvements in their HEI scores, 97% of the participants remained in the worst category. The frequency of participants in the severe BES category dropped markedly postoperatively from 78% to 5% (p < 0.01). Food craving followed a similar trend, with scores dropping from 50 ± 36 pre-surgery to 30 ± 25 post surgery (p < 0.01). Weight regain, prevalent among 40% of participants, was predicted by BES. Despite the improvement in BES and FCI, HEI improvement remained shy. Future interventions should validate findings in other countries and assess means for optimizing HEI scores among BS patients in the Middle East region.
Organic Collaborative Research Partnership Building: Researchers, Needle and Syringe Program Providers, and People Who Inject Drugs
Authors: Resiak Danielle , Mpofu Elias , Rothwell Roderick
Published: October 2021

(1) Background: People who inject drugs (PWID) and needle and syringe program (NSP) providers increasingly partner with researchers to explore harm reduction best practice. However, a paucity of research exists regarding how best to engage PWID and community NSP providers to generate the evidence for sustainable harm reduction services. (2) Aim: This study reports on our use of an organic community research partnership-building approach between researchers, NSP providers, and PWID in Canberra ACT, Australia. (3) Method: Survey participants included both PWID (n = 70) and NSP providers (n = 26) across primary (n = 2), secondary (n = 7), and outreach (n = 1) services in Canberra ACT. Applying an organic partnership-building strategy, we engaged with partners and adapted approaches according to information gained in the process of implementation. (4) Results: We found engaging in relationship building around partner priority activities created mutual understanding and trust premised in authenticity of the evolving partnership. Our organic approach, which included a partner audit of the research tools for relevance, resulted in high acceptance and enrolment into the research by NSP providers and PWID. Finally, we observed strong social capital building utilizing an organic approach for the sustainability of the partnership. (5) Conclusions: The results of this study provide evidence for the benefits of organic collaborative research partnership building with NSP providers and PWID for authentic service program implementation. Our approach to research partnership building resulted in strong relationships built on shared goals and objectives, mutual gains, and complementary expertise. We propose the wider use of organic approaches to developing collaborative research partnerships with NSP providers and PWID to enhance consumer responsiveness towards service provision.
Handling Uncertainty in Cost-Effectiveness Analysis: Budget Impact and Risk Aversion
Authors: Sendi Pedram , Matter-Walstra Klazien , Schwenkglenks Matthias
Published: October 2021

Methods to handle uncertainty in economic evaluation have gained much attention in the literature, and the cost-effectiveness acceptability curve (CEAC) is the most widely used method to summarise and present uncertainty associated with program costs and effects in cost-effectiveness analysis. Some researchers have emphasised the limitations of the CEAC for informing decision and policy makers, as the CEAC is insensitive to radial shifts of the joint distribution of incremental costs and effects in the North-East and South-West quadrants of the cost-effective plane (CEP). Furthermore, it has been pointed out that the CEAC does not incorporate risk-aversion in valuing uncertain costs and effects. In the present article, we show that the cost-effectiveness affordability curve (CEAFC) captures both dimensions of the joint distribution of incremental costs and effects on the CEP and is, therefore, sensitive to radial shifts of the joint distribution on the CEP. Furthermore, the CEAFC also informs about the budget impact of a new intervention, as it can be used to estimate the joint probability that an intervention is both affordable and cost-effective. Moreover, we show that the cost-effectiveness risk-aversion curve (CERAC) allows the analyst to incorporate different levels of risk-aversion into the analysis and can, therefore, be used to inform decision-makers who are risk-averse. We use data from a published cost-effectiveness model of palbociclib in addition to letrozole versus letrozole alone for the treatment of oestrogen-receptor positive, HER-2 negative, advanced breast cancer to demonstrate the differences between CEAC, CEAFC and CERAC, and show how these can jointly be used to inform decision and policy makers.
Development of an Online Asynchronous Clinical Learning Resource (“Ask the Expert”) in Dental Education to Promote Personalized Learning
Authors: Menon Rohit Kunnath , Seow Liang Lin
Published: October 2021

This article describes the development and testing of an online asynchronous clinical learning resource named “Ask the Expert” to enhance clinical learning in dentistry. After the resource development, dental students from years 3 and 4 were randomly allocated to two groups (Group A—“Ask the Expert” and L—“lecturer-led”). All the students attempted a pre-test related to replacement of teeth in the anterior aesthetic zone. Group A (33 students) underwent an online case-based learning session of 60 minutes’ duration without a facilitator, while Group L (27 students) concurrently underwent a case-based learning session of 60 minutes’ duration with a lecturer facilitating the session. An immediate post-test was conducted followed by a retention test after one week. Student feedback was obtained. There was a significant increase in the test scores (maximum score 10) for both groups when comparing the pre-test (Group A—5.61 ± 1.34, Group L—5.22 ± 1.57) and immediate post-test scores (Group A—7.42 ± 1.34, Group L—8.04 ± 1.22; paired t-test, p < 0.001). However, no significant difference was observed in the test scores when comparing Group A to Group L for both the immediate post-test as well as the retention test (Group A—5.36 ± 1.29, Group L—5.33 ± 1.39 (independent sample t-test, p > 0.05). To conclude, adequately structured online asynchronous learning resources are comparable in their effectiveness to online synchronous learning in the undergraduate dental curriculum.
Posttraumatic Stress Disorder and Adjustment Disorder in Lithuanian Healthcare in 2018–2020: A Nation-Wide Cohort Study of the Effects of COVID-19 Pandemic
Authors: Kazlauskas Evaldas , Gelezelyte Odeta , Nomeikaite Auguste , Zelviene Paulina
Published: October 2021

Multiple empirical studies have revealed significant pandemic effects of COVID-19 on mental health in various populations. This study aimed to analyze the incidences of posttraumatic stress disorder (PTSD) and adjustment disorder (AjD) in national healthcare in 2018–2020 in one of the European countries—Lithuania—and estimate the effect of the COVID-19 pandemic on PTSD and AjD incidences in 2020. The national healthcare registry was used for estimations of diagnosis of PTSD, AjD, and major depressive disorder (MD). The study revealed that stress-related disorders PTSD and AjD are diagnosed rarely, resulting in a considerable gap between the expected prevalence and incidences of these diagnoses in healthcare in Lithuania. Moreover, a significant decline in mental disorders incidence in healthcare in 2020, in comparison to 2018 and 2019, was found, revealing that the COVID-19 pandemic had a negative impact on access to healthcare services and increased barriers for mental disorders treatment. The study indicates that major developments in building up knowledge about the effects of trauma and life stressors on mental health are needed in Lithuania and other countries to increase awareness about stress-related disorders and improve care for trauma survivors, in particular in the context of the pandemics or other large-scale disasters.
Survival of COVID-19 with Multimorbidity Patients
Authors: Bustos-Vázquez E. , Padilla-González E. , Reyes-Gómez D. , Carmona-Ramos M. C. , Monroy-Vargas J. A. , Benítez-Herrera A. E. , Meléndez-Mier G.
Published: October 2021

Background: The outbreak of SARS-CoV-2 abruptly disseminated in early 2020, overcoming the capacity of health systems to respond the pandemic. It was not until the vaccines were launched worldwide that an increase in survival was observed. The objectives of this study were to analyse the characteristics of survivors and their relationship with comorbidities. We had access to a database containing information on 16,747 hospitalized patients from Mexico, all infected with SARS-CoV-2, as part of a regular follow-up. The descriptive analysis looked for clusters of either success or failure. We categorized the samples into no comorbidities, or one and up to five coexisting with the infection. We performed a logistic regression test to ascertain what factors were more influential in survival. The main variable of interest was survival associated with multimorbidity factors. The database hosted information on hospitalized patients from Mexico between March 2020 through to April 2021. Categories 2 and 3 had the largest number of patients. Survival rates were higher in categories 0 (64.8%), 1 (57.5%) and 2 (51.6%). In total, 1741 (10.5%) patients were allocated to an ICU unit. Mechanical ventilators were used on 1415 patients, corresponding to 8.76%. Survival was recorded in 9575 patients, accounting for 57.2% of the sample population. Patients without comorbidities, younger people and women were more likely to survive.
Salting the Earth: Intentional Application of Common Salt to Australian Farmland during the Nineteenth Century
Authors: Spennemann Dirk H. R.
Published: October 2021

During the nineteenth century, common salt (NaCl) was liberally applied to Australian farmland as a manure to improve productivity and as a fungicide to prevent, or at least reduce, the impact of rust in wheat. In an age where salinity control is paramount for biodiversity and agricultural productivity alike, it is worth reflecting that during the nineteenth century salt was intentionally applied as a manure to improve soil productivity. This paper traces the origin and extent of this practice in Australia.