bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Dietary Fermented Grape Pomace Supplementation on In Vitro Total Gas and Methane Production, Digestibility, and Rumen Fermentation
Authors: Kara Kanber , Öztaş Mehmet Akif
Published: August 2023

The aim of this study comprises the effect of fermented grape pomace (FGP) in experimental total mixed rations (TMR) at different rates (0, 7.5%, 15%, and 22.5%) on the in vitro cumulative gas production (6th, 12th, 18th and 24th hours), methane production, ruminal fermentation values, pH and ammonia-nitrogen and straight and branched short-chain fatty acids (SCFA and BCFA) concentration. The method of in vitro total gas production was carried out in glass syringes. Ruminal in vitro methane production linearly decreased by adding up to 22.5% FGP in experimental TMR (p < 0.05). The molarities of acetic, propionic, butyric, and valeric acids in the in vitro fermentation fluid linearly decreased with the addition of FGP to TMR (p < 0.05). FGP up to 22.5% in experimental TMRs decreased the molarity of iso-valeric acid and iso-butyric acid from BSCFAs (p < 0.05). As a result, it was concluded that the use of FGP, containing a low level of total condensed tannins (TCTs), up to 22.5% in the experimental TMR based on dry matter (DM) did not adversely affect the in vitro ruminal fermentation value and had an anti-methanogenic effect. In addition, some SCFA (acetic, propionic, butyric, and valeric acids) molarities and iso-acid BSCFA (iso-butyric and iso-valeric acid) did not change up to 15% rate of FGP in the ration. Still, these values decreased by using a 22% rate of FGP. The dose-dependent effect of FGP on ruminal iso-acids has been associated with the ability of TCTs to inhibit ruminal protein degradation partially.
Efficient Scale-Resolving Simulations of Open Cavity Flows for Straight and Sideslip Conditions
Authors: Rajkumar Karthick , Tangermann Eike , Klein Markus
Published: August 2023

This study aims to facilitate a physical understanding of resonating cavity flows with efficient numerical treatments of turbulence. It reinforces the efficiency and affordability of scale-adaptive numerical techniques for simulating open cavity flows with a separated shear layer consisting of a wide range of flow scales. Visualization of the resonant modes occurring due to the acoustic feedback loop aids in a better understanding of large-scale flow oscillations. Under this scope, scale-adaptive simulation (SAS) based on the k-ω SST RANS model with different turbulence treatments has been studied for an open cavity configuration with a length-to-depth (L/D) ratio of 5.7 featuring Mach number (Ma) 0.8 and Reynolds number (Re) 12×106. It is shown that the essential cavity flow physics has been captured using the SAS approach with more than 90% improved computational efficiency compared to commonly used hybrid RANS-LES approaches. In addition, wall-modeled SAS when supplemented with an artificial forcing concept to trigger the model provides very good spectral estimates comparable with hybrid RANS-LES results. Following the validation of numerical approaches, the directional dependence of the cavity resonance is investigated under asymmetric flow conditions, and spanwise interference of waves due to the lateral walls of the cavity has been observed.
Unveiling the Therapeutic Potentials of Mushroom Bioactive Compounds in Alzheimer’s Disease
Authors: Li Na , Li Hongbo , Liu Zhenbin , Feng Gao , Shi Chunyang , Wu Yue
Published: August 2023

Alzheimer’s disease (AD) stands as a prevailing neurodegenerative condition (NDs), leading to the gradual deterioration of brain cells and subsequent declines in memory, thinking, behavior, and emotion. Despite the intensive research efforts and advances, an effective curative treatment for the disease has not yet been found. Mushrooms, esteemed globally for their exquisite flavors and abundant nutritional benefits, also hold a wealth of health-promoting compounds that contribute to improving AD health. These compounds encompass polysaccharides, proteins, lipids, terpenoids, phenols, and various other bioactive substances. Particularly noteworthy are the potent neuroprotective small molecules found in mushrooms, such as ergothioneine, erinacine, flavonoids, alkaloids, ergosterol, and melanin, which warrant dedicated scrutiny for their therapeutic potential in combating AD. This review summarizes such positive effects of mushroom bioactive compounds on AD, with a hope to contribute to the development of functional foods as an early dietary intervention for this neurodegenerative disease.
Cuttlefish Species Authentication: Advancing Label Control through Near-Infrared Spectroscopy as Rapid, Eco-Friendly, and Robust Approach
Authors: Currò Sarah , Balzan Stefania , Novelli Enrico , Fasolato Luca
Published: August 2023

Accurate species identification, especially in the fishery sector, is critical for ensuring food safety, consumer protection and to prevent economic losses. In this study, a total of 93 individual frozen–thawed cuttlefish samples from four different species (S. officinalis, S. bertheloti, S. aculeata, and Sepiella inermis) were collected from two wholesale fish plants in Chioggia, Italy. Species identification was carried out by inspection through morphological features using dichotomic keys and then through near-infrared spectroscopy (NIRS) measurements. The NIRS data were collected using a handled-portable spectrophotometer, and the spectral range scanned was from 900–1680 nm. The collected spectra were processed using principal component analysis for unsupervised analysis and a support vector machine for supervised analysis to evaluate the species identification capability. The results showed that NIRS classification had a high overall accuracy of 93% in identifying the cuttlefish species. This finding highlights the robustness and effectiveness of spectral analysis as a tool for species identification, even in complex spatial contexts. The findings emphasize the potential of NIRS as a valuable tool in the field of fishery product authentication, offering a rapid and eco-friendly approach to species identification in the post-processing stages.
Identification of Potential Bioactive Peptides in Sheep Milk Kefir through Peptidomic Analysis at Different Fermentation Times
Authors: Dalabasmaz Sevim , de la Torre Esther Prados , Gensberger-Reigl Sabrina , Pischetsrieder Monika , Rodríguez-Ortega Manuel J.
Published: August 2023

Sheep farming is an important socioeconomic activity in most Mediterranean countries, particularly Spain, where it contributes added value to rural areas. Sheep milk is used in Spain mainly for making cheese, but it can be used also for making other dairy products, such as the lactic-alcoholic fermentation product known as kefir. Dairy products have health benefits because, among other reasons, they contain molecules with biological activity. In this work, we performed a proteomics strategy to identify the peptidome, i.e., the set of peptides contained in sheep milk kefir fermented for four different periods of time, aiming to understand changes in the pattern of digestion of milk proteins, as well as to identify potential bioactive peptides. In total, we identified 1942 peptides coming from 11 different proteins, and found that the unique peptides differed qualitatively among samples and their numbers increased along the fermentation time. These changes were supported by the increase in ethanol, lactic acid, and D-galactose concentrations, as well as proteolytic activity, as the fermentation progressed. By searching in databases, we found that 78 of the identified peptides, all belonging to caseins, had potential biological activity. Of these, 62 were not previously found in any milk kefir from other animal species. This is the first peptidomic study of sheep milk kefir comprising time-course comparison.
Hydroxypropyl Methylcellulose and Gum Arabic Composite Edible Coatings Amended with Geraniol to Control Postharvest Brown Rot and Maintain Quality of Cold-Stored Plums
Authors: Asgarian Zahra Sadat , Palou Lluís , Souza Ricardo Felipe Lima de , Quintanilla Paloma G. , Taberner Verònica , Karimi Rouhollah , Pérez-Gago María Bernardita
Published: August 2023

In this study, the effect of hydroxypropyl methylcellulose (HPMC) and gum Arabic (GA) edible coatings amended with 0.2% geraniol (GE) were evaluated for the control of brown rot, caused by Monilinia fructicola, on artificially inoculated plums (Prunus salicina Lindl., cv. Angeleno) stored for 5 weeks at 1 °C. Brown rot is the most important pre- and postharvest fungal disease of stone fruits, causing severe economic losses worldwide. Geraniol is an important constituent of many essential oils that can be obtained as a byproduct from different industrial procedures, such as those of the juice industry. Fruit postharvest quality was also evaluated after 5 and 8 weeks of storage at 1 °C, followed by 3 days at 7 °C plus 5 days at 20 °C, simulating packinghouse, transport, and retail shelf-life conditions, respectively. HPMC coatings containing 0.2% GE reduced the incidence and severity of brown rot by 37.5 and 64.8%, respectively, compared to uncoated fruit after 5 weeks of storage at 1 °C. HPMC-coated plums, with and without GE, showed the highest level of firmness, the lowest change in external peel color parameters (L*, a*, b*, C*, hue), and the lowest flesh bleeding compared to uncoated control and GA-coated samples throughout the entire storage period, which correlated with a higher gas barrier of these coatings without negatively affecting sensory quality. Furthermore, the HPMC-0.2% GE coating provided the highest gloss to coated plums, showing the potential of this coating as a safe and environmentally friendly alternative to conventional fungicides and waxes for brown rot control and quality maintenance of cold-stored plums.
A Colorimetric Sensor Enabled with Heterogeneous Nanozymes with Phosphatase-like Activity for the Residue Analysis of Methyl Parathion
Authors: Zhao Fengnian , Li Mengyue , Wang Li , Wang Min
Published: August 2023

In this study, a colorimetric sensor was developed for the detection of organophosphorus pesticides (OPs) using a heterogeneous nanozyme with phosphatase-like activity. Herein, this heterogeneous nanozyme (Au-pCeO2) was obtained by the modification of gold nanoparticles on porous cerium oxide nanorods, resulting in synergistic hydrolysis performance for OPs. Taking methyl parathion (MP) as the target pesticide, the catalytic performance and mechanism of Au-pCeO2 were investigated. Based on the phosphatase-like Au-pCeO2, a dual-mode colorimetric sensor for MP was put forward by the analysis of the hydrolysis product via a UV-visible spectrophotometer and a smartphone. Under optimum conditions, this dual-mode strategy can be used for the on-site analysis of MP with concentrations of 5 to 200 μM. Additionally, it can be applied for MP detection in pear and lettuce samples with recoveries ranging from 85.27% to 115.87% and relative standard deviations (RSDs) not exceeding 6.20%, which can provide a simple and convenient method for OP detection in agricultural products.
The Application of Biometric Approaches in Agri-Food Marketing: A Systematic Literature Review
Authors: Cong Lei , Luan Siqiao , Young Erin , Mirosa Miranda , Bremer Phil , Torrico Damir D.
Published: August 2023

A challenge in social marketing studies is the cognitive biases in consumers’ conscious and self-reported responses. To help address this concern, biometric techniques have been developed to obtain data from consumers’ implicit and non-verbal responses. A systematic literature review was conducted to explore biometric applications’ role in agri-food marketing to provide an integrated overview of this topic. A total of 55 original research articles and four review articles were identified, classified, and reviewed. It was found that there is a steady growth in the number of studies applying biometric approaches, with eye-tracking being the dominant method used to investigate consumers’ perceptions in the last decade. Most of the studies reviewed were conducted in Europe or the USA. Other biometric techniques used included facial expressions, heart rate, body temperature, and skin conductance. A wide range of scenarios concerning consumers’ purchase and consumption behaviour for agri-food products have been investigated using biometric-based techniques, indicating their broad applicability. Our findings suggest that biometric techniques are expanding for researchers in agri-food marketing, benefiting both academia and industry.
Impact of Processing and Physicochemical Parameter on Hibiscus sabdariffa Calyxes Biomolecules and Antioxidant Activity: From Powder Production to Reconstitution
Authors: M’be Cho Urielle , Scher Joël , Gaiani Claire , Amani N’Guessan Georges , Burgain Jennifer
Published: August 2023

Hibiscus sabdariffa is a tropical plant with red calyxes whose anthocyanins, phenols, and antioxidant activity make it attractive to consumers both from a nutritional and medicinal standpoint. Its seasonality, perishability, and anthocyanin instability, led to the setup of stabilization methods comprising drying and powdering. However, its properties can often be altered during these stabilization processes. Treatments such as dehumidified-air-drying, infrared drying, and oven-drying, and their combination showed better quality preservation. Moreover, powder production enables superior biomolecule extractability which can be linked to a higher bioaccessibility. However, the required temperatures for powder production increase the bioactive molecules degradation leading to their antioxidant activity loss. To overcome this issue, ambient or cryogenic grinding could be an excellent method to improve the biomolecule bioavailability and accessibility if the processing steps are well mastered. To be sure to benefit from the final nutritional quality of the powder, such as the antioxidant activity of biomolecules, powders have to offer excellent reconstitutability which is linked to powder physicochemical properties and the reconstitution media. Typically, the finest powder granulometry and using an agitated low-temperature reconstitution media allow for improving anthocyanin extractability and stability. In this review, the relevant physicochemical and processing parameters influencing plant powder features from processing transformation to reconstitution will be presented with a focus on bioactive molecules and antioxidant activity preservation.
Genome Analysis of Bifidobacterium Bifidum E3, Structural Characteristics, and Antioxidant Properties of Exopolysaccharides
Authors: Yue Yingxue , Wang Yuqi , Han Yu , Zhang Yifan , Cao Ting , Huo Guicheng , Li Bailiang
Published: August 2023

In this study, the antioxidant properties of intact cells (IC), cell-free supernatant (CFS), and cell-free extracts (CFE) and whole genome sequencing of Bifidobacterium bifidum E3 (B. bifidum E3), as well as the structural characteristics and antioxidant properties of EPS-1, EPS-2, and EPS-3, were evaluated. The results revealed that intact cells (IC), cell-free supernatant (CFS), and cell-free extracts (CFE) had potent DPPH (1,1-Diphenyl-2-picrylhydrazyl radical), hydroxyl, and superoxide anion radical scavenging capacities, among which CFS was the best. At the genetic level, we identified a strong carbohydrate metabolism capacity, an EPS synthesis gene cluster, and five sugar nucleotides in B. bifidum E3. Therefore, we extracted cEPS from B. bifidum E3 and purified it to obtain EPS-1, EPS-2, and EPS-3. EPS-1, EPS-2, and EPS-3 were heteropolysaccharides with an average molecular weight of 4.15 × 104 Da, 3.67 × 104 Da, and 5.89 × 104 Da, respectively. The EPS-1 and EPS-2 are mainly comprised of mannose and glucose, and the EPS-3 is mainly comprised of rhamnose, mannose, and glucose. The typical characteristic absorption peaks of polysaccharides were shown in Fourier transform infrared spectroscopy (FT-IR spectroscopy). The microstructural study showed a rough surface structure for EPS-1, EPS-2, and EPS-3. Furthermore, EPS-1, EPS-2, and EPS-3 exhibited potent DPPH, hydroxyl, and superoxide anion radical scavenging capacities. Correlation analysis identified that antioxidant capacities may be influenced by various factors, especially molecular weight, chemical compositions, and monosaccharide compositions. In summary, the EPS that was produced by B. bifidum E3 may provide insights into health-promoting benefits in humans.
Toward Sustainable and Healthy Fish Products—The Role of Feeding and Preservation Techniques
Authors: Antonelli Giorgia , Chiarello Elena , Picone Gianfranco , Tappi Silvia , Baldi Giulia , Di Nunzio Mattia , Mente Eleni , Karapanagiotis Stelios , Vasilaki Phelly , Petracci Massimiliano , Rocculi Pietro , Bordoni Alessandra , Capozzi Francesco
Published: August 2023

Fish is a fundamental component of the human diet, and in the near future the proportion of aquatic foods originating from aquaculture production is expected to increase to over 56%. The sustainable growth of the aquaculture sector involves the use of new sustainable raw materials as substitutes for traditional fishmeal and fish oil ingredients, but it is crucial that the substitution maintains the nutritional value of the fish meat. In addition, the preservation of the nutritional value should be a mandatory requirement of new technologies that extend the shelf life of fish. In this context, we evaluated the impact of a newly formulated feed and three preservation treatments (brine, pulsed electric field (PEF), and PEF plus brine) on the fatty acid composition and protein and lipid digestibility of sea bass fillets. In non-digested fillets, although slightly reduced by the newly formulated feed (standard = 2.49 ± 0.14; newly formulated = 2.03 ± 0.10) the n-3/n-6 PUFA ratio indicated good nutritional value. The preservation treatments did not modify the fatty acid content and profile of non-digested fillets. Conversely, protein and lipid digestibility were not affected by the different diets but were significantly reduced by brine, with or without PEF, while PEF alone had no effect. Overall, our results indicated that the newly formulated feed containing 50% less fishmeal is a good compromise between the sustainability and nutritional value of cultivated seabass, and PEF is a promising preservation technology deserving of further study.
Bioactive Compounds of Portuguese Fruits with PDO and PGI
Authors: Farinha Diana , Faustino Hélio , Nunes Catarina , Sales Hélia , Pontes Rita , Nunes João
Published: August 2023

The European Union has established two designations, Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI), to guarantee the authenticity of products with valued specificities associated with the regions where they are produced. The valorization of these products, particularly fruits, not only preserves their natural origins but also promotes the cultivalion of specific regional fruit varieties. This contributes to the preservation of biodiversity and the development of innovative bio-based products in the regions of production. In recent years, substantial efforts have been made to characterize PDO and PGI fruits, particularly in relation to the presence of bioactive compounds with antioxidant and antimicrobial properties. Portugal, with its diverse range of climates and geographical characteristics, is home to numerous fruits with unique flavors, textures, and appearances, many of which are now recognized with PDO or PGI seals. This review compiles data from the production of Portuguese fruits with PDO designations, such as the ‘Maça Bravo de Esmolfe’ (apple) and ‘Pera Rocha do Oeste’ (pear), and PGI designations, such as ‘Citrinos do Algarve’ (citrus)and ‘Cereja da Cova da Beira’ (cherry), and summarizes studies focusing on the bioactive compounds present in these fruits. The presence of bioactive compounds is a significant aspect of nutritious food, associated with health benefits that consumers are increasingly aware of and value.
Understanding the Role of Intraspecific Disease Transmission and Quarantine on the Dynamics of Eco-Epidemiological Fractional Order Model
Authors: Panigoro Hasan S. , Anggriani Nursanti , Rahmi Emli
Published: August 2023

An eco-epidemiological model involving competition regarding the predator and quarantine on infected prey is studied. The prey is divided into three compartments, namely susceptible, infected, and quarantine prey, while the predator only attacks the infected prey due to its weak condition caused by disease. To include the memory effect, the Caputo fractional derivative is employed. The model is validated by showing the existence, uniqueness, non-negativity, and boundedness of the solution. Three equilibrium points are obtained, namely predator-disease-free, predator-free-endemic, and predator-endemic points, which, respectively, represent the extinction of both predator and disease, the extinction of predator only, and the existence of all compartments. The local and global stability properties are investigated using the Matignon condition and the Lyapunov direct method. The numerical simulations using a predictor–corrector scheme are provided not only to confirm the analytical findings but also to explore more the dynamical behaviors, such as the impact of intraspecific competition, memory effect, and the occurrence of bifurcations.
Highly Adhesive Antimicrobial Coatings for External Fixation Devices
Authors: Bredikhin Mikhail , Sawant Sushant , Gross Christopher , Antonio Erik L. S. , Borodinov Nikolay , Luzinov Igor , Vertegel Alexey
Published: August 2023

Pin site infections arise from the use of percutaneous pinning techniques (as seen in skeletal traction, percutaneous fracture pinning, and external fixation for fracture stabilization or complex deformity reconstruction). These sites are niduses for infection because the skin barrier is disrupted, allowing for bacteria to enter a previously privileged area. After external fixation, the rate of pin site infections can reach up to 100%. Following pin site infection, the pin may loosen, causing increased pain (increasing narcotic usage) and decreasing the fixation of the fracture or deformity correction construct. More serious complications include osteomyelitis and deep tissue infections. Due to the morbidity and costs associated with its sequelae, strategies to reduce pin site infections are vital. Current strategies for preventing implant-associated infections include coatings with antibiotics, antimicrobial polymers and peptides, silver, and other antiseptics like chlorhexidine and silver-sulfadiazine. Problems facing the development of antimicrobial coatings on orthopedic implants and, specifically, on pins known as Kirschner wires (or K-wires) include poor adhesion of the drug-eluting layer, which is easily removed by shear forces during the implantation. Development of highly adhesive drug-eluting coatings could therefore lead to improved antimicrobial efficacy of these devices and ultimately reduce the burden of pin site infections. In response to this need, we developed two types of gel coatings: synthetic poly-glycidyl methacrylate-based and natural-chitosan-based. Upon drying, these gel coatings showed strong adhesion to pins and remained undamaged after the application of strong shear forces. We also demonstrated that antibiotics can be incorporated into these gels, and a K-wire with such a coating retained antimicrobial efficacy after drilling into and removal from a bone. Such a coating could be invaluable for K-wires and other orthopedic implants that experience strong shear forces during their implantation.
The Development of the State Emblems and Coats of Arms in Southeast Europe
Authors: Jonovski Jovan
Published: August 2023

Heraldic traditions in southeast European countries are similar, as are the histories of their state emblems and coat of arms. Their development could be classified into three periods: (1) from the founding of the states until the end of World War II; (2) the socialist period; and (3) the period of democratisation after the collapse of socialism. The focus of this work is the processes of the adoption of coats of arms. The descriptions are taken from the appropriate legal documents. This paper examines the emblems and coats of arms of modern southeastern European, or Balkan states, Albania, Bosnia and Herzegovina, Bulgaria, Croatia, Greece, Kosovo, Macedonia, Montenegro, Romania, Slovenia, and Serbia.
Assistive Technology Involving Postural Control and Gait Performance for Adults with Stroke: A Systematic Review and Meta-Analysis
Authors: Hwang Sujin , Song Chiang-Soon
Published: August 2023

This study aimed to comprehensively summarize assistive technology devices for postural control and gait performance in stroke patients. In the study, we searched for randomized controlled trials (RCTs) published until 31 December 2022 in four electrical databases. The most frequently applied assistive technology devices involving postural stability and gait function for stroke patients were robot-assistive technology devices. Out of 1065 initially retrieved citations that met the inclusion criteria, 30 RCTs (12 studies for subacute patients and 18 studies for chronic patients) were included in this review based on eligibility criteria. The meta-analysis included ten RCTs (five studies for subacute patients and five for chronic patients) based on the inclusion criteria of the data analysis. After analyzing, the variables, only two parameters, the Berg balance scale (BBS) and the functional ambulation category (FAC), which had relevant data from at least three studies measuring postural control and gait function, were selected for the meta-analysis. The meta-analysis revealed significant differences in the experimental group compared to the control group for BBS in both subacute and chronic stroke patients and for the FAC in chronic stroke patients. Robot-assistive training was found to be superior to regular therapy in improving postural stability for subacute and chronic stroke patients but not gait function. This review suggests that robot-assistive technology devices should be considered in rehabilitative approaches for postural stability and gait function for subacute and chronic stroke patients.
Ultrasound Imaging of Subtalar Joint Instability for Chronic Ankle Instability
Authors: Kudo Shintarou , Aoyagi Tsutomu , Kobayashi Takumi , Koshino Yuta , Edama Mutsuaki
Published: August 2023

The purpose of this study was to develop the assessment of subtalar joint instability with chronic ankle instability (CAI) using ultrasonography. Forty-six patients with anterior talofibular ligament (ATFL) abnormalities and a history of ankle sprain were divided into CAI (21.2 ± 5.9 y/o, 7 males and 17 females) and asymptomatic groups (21.0 ± 7.4 y/o, 9 males and 12 females) on the basis of subjective ankle instability assessed using the CAIT and the Ankle Instability Instrument Tool (AIIT). Twenty-six age-matched feet participated in a control group (18.9 ± 7.0 y/o, 9 males and 17 females). Ultrasound measurements of the width of the posterior subtalar joint facet were obtained at rest and maximum ankle inversion (subtalar joint excursion; STJE). The differences in STJE among the three groups were assessed by one-way ANOVA. The relationship between STJE and subjective ankle instability was assessed using Spearman’s correlation tests. The STJE value was significantly greater in the CAI group (2.3 ± 0.8 mm) than in the asymptomatic (1.0 ±0.4 mm) and control groups (0.8 ±0.2 mm) (p < 0.001, effect size: 0.64). STJE had significant negative correlations with CAIT (r = −0.71, p < 0.01), and significant positive correlations with AIIT (r = 0.74, p < 0.01). The cut-off value to distinguish between the CAI and asymptomatic groups was 1.7 mm using the ROC curve.
Classification of Color Fundus Photographs Using Fusion Extracted Features and Customized CNN Models
Authors: Wang Jing-Zhe , Lu Nan-Han , Du Wei-Chang , Liu Kuo-Ying , Hsu Shih-Yen , Wang Chi-Yuan , Chen Yun-Ju , Chang Li-Ching , Twan Wen-Hung , Chen Tai-Been , Huang Yung-Hui
Published: August 2023

This study focuses on overcoming challenges in classifying eye diseases using color fundus photographs by leveraging deep learning techniques, aiming to enhance early detection and diagnosis accuracy. We utilized a dataset of 6392 color fundus photographs across eight disease categories, which was later augmented to 17,766 images. Five well-known convolutional neural networks (CNNs)—efficientnetb0, mobilenetv2, shufflenet, resnet50, and resnet101—and a custom-built CNN were integrated and trained on this dataset. Image sizes were standardized, and model performance was evaluated via accuracy, Kappa coefficient, and precision metrics. Shufflenet and efficientnetb0demonstrated strong performances, while our custom 17-layer CNN outperformed all with an accuracy of 0.930 and a Kappa coefficient of 0.920. Furthermore, we found that the fusion of image features with classical machine learning classifiers increased the performance, with Logistic Regression showcasing the best results. Our study highlights the potential of AI and deep learning models in accurately classifying eye diseases and demonstrates the efficacy of custom-built models and the fusion of deep learning and classical methods. Future work should focus on validating these methods across larger datasets and assessing their real-world applicability.
Violence Prevention Climate and Health-Oriented Leadership in German Emergency Departments
Authors: Guliani Mannat , Reißmann Sonja , Westenhöfer Joachim , Harth Volker , Mache Stefanie
Published: August 2023

Emergency departments (EDs) are high-risk environments for workplace violence. Research into techniques to prevent violence has less frequently explored the influence of leadership. This study aims to analyze the association of leadership with the prevention of violence using the concepts of health-oriented leadership (HoL) and the violence prevention climate (VPC). This quantitative cross-sectional study was conducted through online surveys between November 2021 and March 2022 across Germany. A sample of 370 doctors and nurses working in German EDs were recruited. Perceptions towards VPC and HoL were compared between groups divided according to profession and position using independent t-tests or Mann–Whitney U tests. Separate multiple linear regression models for supervisors and employees analyzed the association between different profiles of HoL with VPC. Supervisors and employees showed significant differences in supervisor staff-care and VPC. Regression analysis demonstrated that supervisors’ self-care and employees’ assessment of supervisor’s staff-care positively predicted all dimensions of VPC. This empirical study provides insights into the variable perceptions of different groups and the association of leadership profiles with the perceptivity of VPC. The results of this study can be used to emphasize the importance of HoL training for both employees and supervisors to improve communication and health-promoting behavior.
Correlation of the Grapevine (Vitis vinifera L.) Leaf Chlorophyll Concentration with RGB Color Indices
Authors: Bodor-Pesti Péter , Taranyi Dóra , Nyitrainé Sárdy Diána Ágnes , Le Phuong Nguyen Lien , Baranyai László
Published: August 2023

Spectral investigation of the canopy has an increasing importance in precision viticulture to monitor the effect of biotic and abiotic stress factors. In this study, RGB (color model, red, green, blue)-based vegetation indices were evaluated to find a correlation with grapevine leaf chlorophyll concentration. ‘Hárslevelű’ (Vitis vinifera L.) leaf samples were obtained from a commercial vineyard and digitalized. The chlorophyll concentration of the samples was determined with a portable chlorophyll meter. Image processing and color analyses were performed to determine the RGB average values of the digitized samples. According to the RGB values, 31 vegetation indices were calculated and evaluated with a correlation test and multivariate regression. The Pearson correlation between the chlorophyll concentration and most of the indices was significant (p < 0.01), with some exceptions. The same results were obtained with the Spearman correlation as the relationship had high significance (p < 0.01) for most of the indices. The highest Pearson correlation was obtained with the index PCA2 (Principal Component Analysis 2), while Spearman correlation was the highest for RMB (difference between red and blue) and GMB (difference between green and blue). The multivariate regression model also showed a high correlation with the pigmentation. We consider that our results would be applicable in the future to receive information about the canopy physiological status monitored with on-the-go sensors.
Effects of Orchard Grass on Soil Fertility and Nutritional Status of Fruit Trees in Korla Fragrant Pear Orchard
Authors: Wang Zengheng , Liu Rui , Fu Liang , Tao Shutian , Bao Jianping
Published: August 2023

Grass cultivation is widely used as an effective soil management method in pear orchards. A Korla fragrant pear orchard with clean cultivation (CK) and natural grass planting for 1, 2, 3, 4, and 5 years was examined in this study. We analyzed the differences in soil fertility and shoot and leaf nutrient content under different grassing years. Compared with the clean cultivation, grass cultivation reduced the soil fertility and nutrient content of shoots and leaves at the early stage of natural grass planting (1–2 years). With the increase in grassing years, the overall level of the nutrient content of soil, shoots, and leaves gradually increased; the shallower the soil layer, the more significant the effect. Furthermore, grass cultivation significantly increased the contents of soil organic matter, total nitrogen, total phosphorus, total potassium, alkali-hydrolyzable nitrogen, available phosphorus, and available potassium. The nutrient contents of shoots and leaves were also significantly increased after grass cultivation. The contents of total nitrogen, total phosphorus, and total potassium in shoots were significantly increased by 7.32%, 154.84%, and 219.29% in 5, 4, and 5 years, respectively, and their contents were also increased by 69.57%, 22.86%, and 26.45% in leaves. The correlation analysis showed that there was a significant or extremely significant positive correlation among the shoots, leaves, and soil nutrient contents. In conclusion, continuous grass cultivation significantly improved the soil quality, health status, and nutritional status, and effectively solved the problem of harm to the pear orchard caused by long-term clean cultivation. This study will provide the scientific basis for the construction of a reasonable orchard soil management mode.
Cloning and Functional Analysis of NtHDR in ‘Jinzhanyintai’ of Narcissus tazetta var. chinensis M.Roem
Authors: Hu Xiaomeng , Zhang Na , Fan Keke , Chang Yanting , Zhang Wenbo , Deng Yayun , Wang Shuhua , Hu Tao , Ma Yanjun
Published: August 2023

The gene encoding 1-hydroxy-2-methyl-2-(E)-butenyl-4-pyrophosphate reductase, a key enzyme at the end of the methylerythritol 4-phosphate pathway (MEP), was cloned from the petals of Narcissus tazetta var. Chinensis ‘Jinzhanyintai’ and named NtHDR (accession number OQ739816). NtHDR exhibits a full-length coding sequence spanning 1380 base pairs, encoding a total of 460 amino acids. Through phylogenetic-tree analysis, it has been determined that NtHDR shared the closest evolutionary relationship with monocotyledons, specifically Asparagus officinalis and Zingiber officinale. Utilizing quantitative real-time PCR analysis, it was observed that NtHDR exhibited significant expression in both petals and corona, with expression levels varying throughout the flowering process of Narcissus. Specifically, in petals, NtHDR expression demonstrated a pattern of initial increase followed by subsequent decrease, while in corona, it consistently increased. Subsequent subcellular-localization experiments indicated that NtHDR was localized within the chloroplasts. To investigate the functional impact of NtHDR, a stable transformation was performed, where NtHDR was introduced into Nicotiana benthamiana. The resulting transgenic N. benthamiana flowers were analyzed using solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME-GC-MS) to characterize their volatile components. The analysis revealed the presence of the monoterpene compound linalool, as well as the phenylpropanoids benzyl alcohol and phenylethanol, within the floral fragrance components of the transgenic N. benthamiana plants. However, these compounds were absent in the floral fragrance components of wild-type tobacco plants, thus highlighting the impact of NtHDR transformation on the floral scent profile.
Metric and Color Modifications for the Automated Construction of Map Symbols
Authors: Gong Xinyu , Lan Tian , Ti Peng
Published: August 2023

Personalized mappings become popular among the public with the support of data diversity and device diversity. To develop personalized maps, constructing map symbols through automated ways is beneficial. The formal representation of map symbols (i.e., expressing map symbols by mathematical operators) is fundamental to the automated construction of map symbols. A previous study to evaluate the feasibility of structures of Chinese characters for representing map symbols shows that 77.5% of map symbols can be represented by them, although there are imperfections in some cases. It means that: (1) the other 22.5% of symbols should be formally represented by other mathematical solutions, and (2) those imperfect cases should be made perfect through some modification or refinements. In this study, we solve the representation problems of these two types of map symbols (i.e., the map symbol did not or imperfectly fit the structures of Chinese characters) by employing additional basic operators and proposing some metric and color modifications. To validate these proposed solutions, experiments have been carried out by using eight sets of symbols that are publicly available (e.g., Google Icons). The results indicated that almost all the map symbols can be formally represented with additional operators and metric and color modifications. The percentages of map symbols that did not fit structures of Chinese characters solved by these operators and modifications are 2.4% and 20.1%, respectively. The percentages of map symbols that imperfectly fit them solved by these operators and modifications are 8.7% and 8%, respectively. This work could not only enrich cartographic theory but also prompt the mathematization of map symbol construction.
Application of Human Brain Organoids—Opportunities and Challenges in Modeling Human Brain Development and Neurodevelopmental Diseases
Authors: Kim Soo-hyun , Chang Mi-Yoon
Published: August 2023

Brain organoids are three-dimensional (3D) structures derived from human pluripotent stem cells (hPSCs) that reflect early brain organization. These organoids contain different cell types, including neurons and glia, similar to those found in the human brain. Human brain organoids provide unique opportunities to model features of human brain development that are not well-reflected in animal models. Compared with traditional cell cultures and animal models, brain organoids offer a more accurate representation of human brain development and function, rendering them suitable models for neurodevelopmental diseases. In particular, brain organoids derived from patients’ cells have enabled researchers to study diseases at different stages and gain a better understanding of disease mechanisms. Multi-brain regional assembloids allow for the investigation of interactions between distinct brain regions while achieving a higher level of consistency in molecular and functional characterization. Although organoids possess promising features, their usefulness is limited by several unresolved constraints, including cellular stress, hypoxia, necrosis, a lack of high-fidelity cell types, limited maturation, and circuit formation. In this review, we discuss studies to overcome the natural limitations of brain organoids, emphasizing the importance of combinations of all neural cell types, such as glia (astrocyte, oligodendrocytes, and microglia) and vascular cells. Additionally, considering the similarity of organoids to the developing brain, regionally patterned brain organoid-derived neural stem cells (NSCs) could serve as a scalable source for cell replacement therapy. We highlight the potential application of brain organoid-derived cells in disease cell therapy within this field.
Tackling Sleeping Sickness: Current and Promising Therapeutics and Treatment Strategies
Authors: Jamabo Miebaka , Mahlalela Maduma , Edkins Adrienne L. , Boshoff Aileen
Published: August 2023

Human African trypanosomiasis is a neglected tropical disease caused by the extracellular protozoan parasite Trypanosoma brucei, and targeted for eradication by 2030. The COVID-19 pandemic contributed to the lengthening of the proposed time frame for eliminating human African trypanosomiasis as control programs were interrupted. Armed with extensive antigenic variation and the depletion of the B cell population during an infectious cycle, attempts to develop a vaccine have remained unachievable. With the absence of a vaccine, control of the disease has relied heavily on intensive screening measures and the use of drugs. The chemotherapeutics previously available for disease management were plagued by issues such as toxicity, resistance, and difficulty in administration. The approval of the latest and first oral drug, fexinidazole, is a major chemotherapeutic achievement for the treatment of human African trypanosomiasis in the past few decades. Timely and accurate diagnosis is essential for effective treatment, while poor compliance and resistance remain outstanding challenges. Drug discovery is on-going, and herein we review the recent advances in anti-trypanosomal drug discovery, including novel potential drug targets. The numerous challenges associated with disease eradication will also be addressed.
T Follicular Helper Cells in Tertiary Lymphoid Structure Contribute to Renal Fibrosis by IL-21
Authors: Luo Ran , Chang Dan , Zhang Nanhui , Cheng Yichun , Ge Shuwang , Xu Gang
Published: August 2023

Tertiary lymphoid structure (TLS) represents lymphocyte clusters in non-lymphoid organs. The formation and maintenance of TLS are dependent on follicular helper T (TFH) cells. However, the role of TFH cells during renal TLS formation and the renal fibrotic process has not been comprehensively elucidated in chronic kidney disease. Here, we detected the circulating TFH cells from 57 IgAN patients and found that the frequency of TFH cells was increased in IgA nephropathy patients with renal TLS and also increased in renal tissues from the ischemic-reperfusion-injury (IRI)-induced TLS model. The inducible T-cell co-stimulator (ICOS) is one of the surface marker molecules of TFH. Remarkably, the application of an ICOS-neutralizing antibody effectively prevented the upregulation of TFH cells and expression of its canonical functional mediator IL-21, and also reduced renal TLS formation and renal fibrosis in IRI mice in vivo. In the study of this mechanism, we found that recombinant IL-21 could directly promote renal fibrosis and the expression of p65. Furthermore, BAY 11-7085, a p65 selective inhibitor, could effectively alleviate the profibrotic effect induced by IL-21 stimulation. Our results together suggested that TFH cells contribute to TLS formation and renal fibrosis by IL-21. Targeting the ICOS-signaling pathway network could reduce TFH cell infiltration and alleviate renal fibrosis.
The Crossroads of the Coagulation System and the Immune System: Interactions and Connections
Authors: Wilhelm Grzegorz , Mertowska Paulina , Mertowski Sebastian , Przysucha Anna , Strużyna Jerzy , Grywalska Ewelina , Torres Kamil
Published: August 2023

The coagulation and immune systems, two vital systems in the human body, share intimate connections that fundamentally determine patient health. These systems work together through several common regulatory pathways, including the Tissue Factor (TF) Pathway. Immune cells expressing TF and producing pro-inflammatory cytokines can influence coagulation, while coagulation factors and processes reciprocally impact immune responses by activating immune cells and controlling their functions. These shared pathways contribute to maintaining health and are also involved in various pathological conditions. Dysregulated coagulation, triggered by infection, inflammation, or tissue damage, can result in conditions such as disseminated intravascular coagulation (DIC). Concurrently, immune dysregulation may lead to coagulation disorders and thrombotic complications. This review elucidates these intricate interactions, emphasizing their roles in the pathogenesis of autoimmune diseases and cancer. Understanding the complex interplay between these systems is critical for disease management and the development of effective treatments. By exploring these common regulatory mechanisms, we can uncover innovative therapeutic strategies targeting these intricate disorders. Thus, this paper presents a comprehensive overview of the mutual interaction between the coagulation and immune systems, highlighting its significance in health maintenance and disease pathology.
GV1001 Inhibits the Severity of the Ligature-Induced Periodontitis and the Vascular Lipid Deposition Associated with the Periodontitis in Mice
Authors: Kim Sharon Y. , Kim Yun-Jeong , Kim Suyang , Momeni Mersedeh , Lee Alicia , Treanor Alexandra , Kim Sangjae , Kim Reuben H. , Park No-Hee
Published: August 2023

GV1001, a 16 amino acid peptide derived from the catalytic segment of human telomerase reverse transcriptase, was developed as an anti-cancer vaccine. Subsequently, it was found to exhibit anti-inflammatory and anti-Alzheimer’s disease properties. Periodontitis is a risk factor for a variety of systemic diseases, including atherosclerosis, a process in which chronic systemic and vascular inflammation results in the formation of plaques containing lipids, macrophages, foam cells, and tissue debris on the vascular intima. Thus, we investigated the effect of GV1001 on the severity of ligature-induced periodontitis, vascular inflammation, and arterial lipid deposition in mice. GV1001 notably reduced the severity of ligature-induced periodontitis by inhibiting gingival and systemic inflammation, alveolar bone loss, and vascular inflammation in wild-type mice. It also significantly lowered the amount of lipid deposition in the arterial wall in ApoE-deficient mice receiving ligature placement without changing the serum lipid profile. In vitro, we found that GV1001 inhibited the Receptor Activator of NF-κB ligand (RANKL)-induced osteoclast formation and tumor necrosis factor-α (TNF-α)-induced phenotypic changes in endothelial cells. In conclusion, our study suggests that GV1001 prevents the exacerbation of periodontitis and atherosclerosis associated with periodontitis partly by inhibiting local, systemic, and vascular inflammation and phenotypic changes of vascular endothelial cells.