bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Different Downward Loads on Canal Centering Ability, Vertical Force, and Torque Generation during Nickel–Titanium Rotary Instrumentation
Authors: Maki Keiichiro , Ebihara Arata , Unno Hayate , Omori Satoshi , Nakatsukasa Taro , Kimura Shunsuke , Okiji Takashi
Published: April 2022

This study aimed to examine how downward loads influence the torque/force and shaping outcome of ProTaper NEXT (PTN) rotary instrumentation. PTN X1, X2, and X3 were used to prepare J-shaped resin canals employing a load-controlled automated instrumentation and torque/force measuring device. Depending on the torque values, the handpiece was programmed to move as follows: up and down; downward at a preset downward load of 1 N, 2 N or 3 N (Group 1N, 2N, and 3N, respectively; each n = 10); or upward. The torque/force values and instrumentation time were recorded, and the canal centering ratio was calculated. The results were analyzed using a two-way or one-way analysis of variance and the Tukey test (α = 0.05). At the apex level, Group 3N exhibited the least canal deviation among the three groups (p < 0.05). The downward force was Group 3N > Group 2N > Group 1N (p < 0.05). The upward force, representing the screw-in force, was Group 3N > Group 1N (p < 0.05). The total instrumentation time was Group 1N > Group 3N (p < 0.05). In conclusion, increasing the downward load during PTN rotary instrumentation improved the canal centering ability, reduced the instrumentation time, and increased the upward force.
The Improvement of Durability of Reinforced Concretes for Sustainable Structures: A Review on Different Approaches
Authors: Coppola Luigi , Beretta Silvia , Bignozzi Maria Chiara , Bolzoni Fabio , Brenna Andrea , Cabrini Marina , Candamano Sebastiano , Caputo Domenico , Carsana Maddalena , Cioffi Raffaele , Coffetti Denny , Colangelo Francesco , Crea Fortunato , De Gisi Sabino , Diamanti Maria Vittoria , Ferone Claudio , Frontera Patrizia , Gastaldi Matteo Maria , Labianca Claudia , Lollini Federica , Lorenzi Sergio , Manzi Stefania , Marroccoli Milena , Notarnicola Michele , Ormellese Marco , Pastore Tommaso , Pedeferri MariaPia , Petrella Andrea , Redaelli Elena , Roviello Giuseppina , Telesca Antonio , Todaro Francesco
Published: April 2022

The topic of sustainability of reinforced concrete structures is strictly related with their durability in aggressive environments. In particular, at equal environmental impact, the higher the durability of construction materials, the higher the sustainability. The present review deals with the possible strategies aimed at producing sustainable and durable reinforced concrete structures in different environments. It focuses on the design methodologies as well as the use of unconventional corrosion-resistant reinforcements, alternative binders to Portland cement, and innovative or traditional solutions for reinforced concrete protection and prevention against rebars corrosion such as corrosion inhibitors, coatings, self-healing techniques, and waterproofing aggregates. Analysis of the scientific literature highlights that there is no preferential way for the production of “green” concrete but that the sustainability of the building materials can only be achieved by implementing simultaneous multiple strategies aimed at reducing environmental impact and improving both durability and performances.
Using Artificial Intelligence Techniques to Predict Punching Shear Capacity of Lightweight Concrete Slabs
Authors: Ebid Ahmed , Deifalla Ahmed
Published: April 2022

Although lightweight concrete is implemented in many mega projects to reduce the cost and improve the project’s economic aspect, research studies focus on investigating conventional normal-weight concrete. In addition, the punching shear failure of concrete slabs is dangerous and calls for precise and consistent prediction models. Thus, this current study investigates the prediction of the punching shear strength of lightweight concrete slabs. First, an extensive experimental database for lightweight concrete slabs tested under punching shear loading is gathered. Then, effective parameters are determined by applying the principles of statistical methods, namely, concrete density, columns dimensions, slab effective depth, concrete strength, flexure reinforcement ratio, and steel yield stress. Next, the manuscript presented three artificial intelligence models, which are genetic programming (GP), artificial neural network (ANN) and evolutionary polynomial regression (EPR). In addition, it provided guidance for future design code development, where the importance of each variable on the strength was identified. Moreover, it provided an expression showing the complicated inter-relation between affective variables. The novelty lies in developing three proposed models for the punching capacity of lightweight concrete slabs using three different (AI) techniques capable of accurately predicting the strength compared to the experimental database
Experimental Analysis of the Effect of Wear Factors on Guide Vane of Hydraulic Turbine
Authors: Zhao Xiaoyi , Peng Yuanjie , Yang Junxiang , Ren Minghui , Liu Xiaobing
Published: April 2022

In this paper, in order to study the wear of the guide vane of the hydraulic turbine, a test bench was built according to the actual internal flow of the hydraulic turbine of the power station. The value of the wear of the surface of the guide vane after polishing was reduced by 18.1 μm compared with that before polishing of P = 30.9 MW and by 12.5 μm at P = 42.8 MW. In order to reduce the influence of sediment wear on the guide vane, a 0.3 mm thick tungsten carbide coating was sprayed on its surface, and the wear of the guide vane after spraying tungsten carbide was obtained. The wear of the guide vane was reduced by about a factor of three to four times compared with that before spraying. In addition, according to the pH value of 6.73 of the river where the power station is located, the change of dissolved oxygen in the water body will affect the wear of the metal material on the surface of the guide vane, and the dissolved oxygen varies with the change of the water body temperature, so we simulated the temperature of the water body in the flood and the dry period of the power station, and got the wear amount of the polished guide vane in the flood period under the two working conditions of 28.1 μm and 47.3 μm, respectively. The wear amounts of the guide vane in the dry period were 25.2 μm and 43.9 μm, respectively. In addition, the service life of the guide vane before and after polishing and after tungsten carbide spraying was estimated based on the wear data obtained from the test, which provides a basis for power plant maintenance.
Graphene-Coated Iron Nitride Streptavidin Magnetic Beads: Preparation and Application in SARS-CoV-2 Enrichment
Authors: Li Jianxing , Wang Minglian , Jia Runqing , Ma Zhuang , Zhang Xiaoxu , Li Jintao , Xiao Xiangqian , Zhou Yunzhi , Wang Qun
Published: April 2022

In this study, we prepared a streptavidin magnetic bead based on graphene-coated iron nitride magnetic beads (G@FeN-MB) and tried to use it for the enrichment of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The outer shell of our magnetic bead was wrapped with multiple graphene sheets, and there is no report on the application of graphene to the magnetic-bead-coating material. First, the graphene shell of G@FeN-MB was oxidized by a modified Hummer method so as to generate the carboxyl groups required for the coupling of streptavidin (SA) on the surface of the magnetic beads. X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) were used to characterize the oxidized G@FeN-MB (GO@FeN-MB). Streptavidin was then linked to the surface of the GO@FeN-MB by coupling the amino of the streptavidin with the carboxyl on the magnetic beads by carbodiimide method; thus, the streptavidin magnetic beads (SAMBs) were successfully prepared. To prove the practicality of the SAMBs, biotinylated SARS-CoV-2 S1 antibody was linked with it to respectively capture SARS-CoV-2 Spike-protein-coupled polystyrene beads (S-PS) and pseudovirus with S-protein expressed. Microplate reader and fluorescence microscope results show that the SAMBs can effectively enrich viruses. In conclusion, the preparation of SAMBs with G@FeN-MB is feasible and has potential for application in the field of virus enrichment.
Metamorphoses of Earnings in the Transport Sector of the V4 Region
Authors: Durana Pavol , Valaskova Katarina , Blazek Roman , Palo Jozef
Published: April 2022

The transportation sector is a crucial sector of the sustainability of every national economy. Previous studies highlighted the core significance of transport enterprises in European countries over the past 60 years. The long-term sustainability of enterprises is determined by their ability to gain earnings. Thus, earnings are the synonym of significance in corporate life. The purpose of this study was to capture the lever year, the trend, and the slope of the development of earnings in the transport sector before the COVID-19 pandemic. Time series of the annual earnings of the enterprises from the close countries of the V4 region were used during a 10-year period. Buishand’s test sets the change-points of the development and indicated the values of specific central lines. The year 2013 was the lever date for the earnings of 830 Slovak and 1042 Hungarian enterprises. The year 2015 was the year of momentum for 757 Polish enterprises. The development of 397 Czech enterprises was mainly influenced by the year 2014. The results of the Mann–Kendall test detected a positive trend in the series of business finance in all countries. In addition, the Sen’s slope was estimated in the transport sector for the analyzed period 2010–2019.
Zeroing Neural Network for Pseudoinversion of an Arbitrary Time-Varying Matrix Based on Singular Value Decomposition
Authors: Kornilova Mariya , Kovalnogov Vladislav , Fedorov Ruslan , Zamaleev Mansur , Katsikis Vasilios N. , Mourtas Spyridon D. , Simos Theodore E.
Published: April 2022

Many researchers have investigated the time-varying (TV) matrix pseudoinverse problem in recent years, for its importance in addressing TV problems in science and engineering. In this paper, the problem of calculating the inverse or pseudoinverse of an arbitrary TV real matrix is considered and addressed using the singular value decomposition (SVD) and the zeroing neural network (ZNN) approaches. Since SVD is frequently used to compute the inverse or pseudoinverse of a matrix, this research proposes a new ZNN model based on the SVD method as well as the technique of Tikhonov regularization, for solving the problem in continuous time. Numerical experiments, involving the pseudoinversion of square, rectangular, singular, and nonsingular input matrices, indicate that the proposed models are effective for solving the problem of the inversion or pseudoinversion of time varying matrices.
A Note on the Concept of Time in Extensive Games
Authors: Luckraz Shravan , Pansera Bruno Antonio
Published: April 2022

Using the concept of informational digraphs, we propose a “no redundant information sets” property that can characterize the exact class of extensive games which can be time structured. Our result can be applied to define time-dependent solution concepts like the Open-Loop and the Closed-Loop Nash Equilibrium in extensive games with imperfect information.
Factors Associated with the Refusal of Direct-Acting Antiviral Agents for the Treatment of Hepatitis C in Taiwan
Authors: Chang Li-Jen , Chang Han-Cheng , Chen Po-Yueh , Chen Chi-Yi , Tsai Kun-Feng , Yean Koh-Kok , Yang Hsin-Yi , Chen Tsung-Hsien , Yu Pao-Ta , Chou Chu-Kuang , Chen Sheng-Hsuan
Published: April 2022

Background and Objectives: Direct-acting antiviral agents (DAA) are a safe and highly effective treatment for hepatitis C virus (HCV) infection. However, the uptake of DAA treatment remains a challenge. This study aims to examine the reasons for DAA refusal among HCV patients covered by the Taiwan National Health Insurance system. Materials and Methods: This retrospective observational study covered the period from January 2009 to December 2019 and was conducted at a single hepatitis treatment center in Taiwan. This study involved chart reviews and phone-based surveys to confirm treatment status and refusal causes. To confirm treatment status, subjects with HCV without treatment records were phone-contacted to confirm treatment status. Patients who did not receive treatment were invited back for treatment. If the patient refused, the reason for refusal was discussed. Results: A total of 3566 patients were confirmed with DAA treatment; 418 patients (179 patients who were lost to contact or refused the survey and 239 patients who completed the survey of DAA refusal) were included in the no-DAA-therapy group. Factors associated with receiving DAAs were hemoglobin levels, hepatitis B virus co-infection, and regular gastroenterology visits. Meanwhile, male sex, platelet levels, and primary care physician visits were associated with DAA refusal. The leading causes of treatment refusal were multiple comorbidities, low health literacy, restricted access to hospitals, nursing home residence, and old age. The rate of DAA refusal remains high (10%). Conclusions: The reasons for treatment refusal are multifactorial, and addressing them requires complex interventions.
Pollution and Cleaning of PDMS Pervaporation Membranes after Recovering Ethyl Acetate from Aqueous Saline Solutions
Authors: Sun Xuefei , Pan Yang , Shen Chunxiang , Zuo Chengye , Ding Xiaobin , Liu Gongping , Xing Weihong , Jin Wanqin
Published: April 2022

The removal of volatile organic compounds (VOCs) from wastewater containing nonvolatile salts has become an important and interesting case of the application of the pervaporation (PV) process. The aim of this study was to evaluate the influence of salts on the PV removal of ethyl acetate from wastewater using a polydimethylsiloxane (PDMS) membrane. The fouled membrane was then characterized via scanning electron microscopy–energy-dispersive X-ray analysis (SEM–EDX) to investigate salt permeation. The membrane backflushing process was carried out by periodically flushing the permeate side of the tubular membrane. The results demonstrated that salts (NaCl and CaCl2) could permeate through the PDMS membrane and were deposited on the permeate side. The presence of salts in the feed solution caused a slight increase in the membrane selectivity and a decrease in the permeate flux. The flux decreased with increasing salt concentration, and a notable effect occurred at higher feed-salt concentrations. A permeate flux of up to 98.3% of the original flux was recovered when the permeation time and backflushing duration were 30 and 5 min, respectively, indicating that the effect of salt deposition on flux reduction could be mitigated. Real, organic, saline wastewater was treated in a pilot plant, which further verified the feasibility of wastewater PV treatment.
MXene (Ti3C2Tx)/Cellulose Acetate Mixed-Matrix Membrane Enhances Fouling Resistance and Rejection in the Crossflow Filtration Process
Authors: Azam Reem S. , Almasri Dema A. , Alfahel Radwan , Hawari Alaa H. , Hassan Mohammad K. , Elzatahry Ahmed A. , Mahmoud Khaled A.
Published: April 2022

Obstacles in the membrane-based separation field are mainly related to membrane fouling. This study involved the synthesis and utilization of covalently crosslinked MXene/cellulose acetate mixed matrix membranes with MXene at different concentrations (CCAM-0% to CCAM-12%) for water purification applications. The membranes’ water flux, dye, and protein rejection performances were compared using dead-end (DE) and crossflow (CF) filtration. The fabricated membranes, especially CCAM-10%, exhibited high hydrophilicity, good surface roughness, significantly high water flux, high water uptake, and high porosity. A significantly higher flux was observed in CF filtration relative to DE filtration. Moreover, in CF filtration, the CCAM-10% membrane exhibited 96.60% and 99.49% rejection of methyl green (MG) and bovine serum albumin (BSA), respectively, while maintaining a flux recovery ratio of 67.30% and an irreversible fouling ratio at (Rir) of 32.70, indicating good antifouling performance. Hence, this study suggests that covalent modification of cellulose acetate membranes with MXene significantly improves the performance and fouling resistance of membranes for water filtration in CF mode relative to DE mode.
Study of the Double-Layer Sintering Process with Stand-Support
Authors: Liu Jie , Zhou Mingshun , Wu Fadeng , Zhang Hui , Xu Libing , Zhai Liwei , Gao Wei , Zhong Qiang
Published: April 2022

It has been widely reported that the sintering productivity and sintering bed permeability would be reduced when adopting ultra-deep bed sintering. To solve the aforementioned problems, the double-layer sintering process with stand-support (DLSP-S) is proposed in this research to achieve the sintering of a 1000 mm ultra-deep bed. The results showed that compared with the double-layer sintering process, the DLSP-S improved the yield and productivity of sintering from 64.53% and 1.76 t·m−2·h−1 to 66.74% and 2.12 t·m−2·h−1, respectively. The research findings showed that the quasi-fines of 5–10 mm were reduced by 2.1% when the stand height increased from 0 mm to 350 mm, which further illustrated the effect of the DLSP-S. During the DLSP-S, the air permeability of the sinter bed was evidently improved and the content of O2 in the lower layer was enhanced. The present study provides an effective approach to improve the bed permeability and sintering productivity in high-bed sintering.
Effect of Mechanical Properties of Rail and Wheel on Wear and Rolling Contact Fatigue
Authors: Seo Jung-Won , Hur Hyun-Moo , Kwon Seok-Jin
Published: April 2022

Rolling contact fatigue (RCF) and wear are important problems for the wheel and rail. RCF and wear is caused by contact stress and the slip ratio between the wheel and the rail. The material properties of the wheel and rail are an important factor to prevent the degradation caused by RCF and wear. In this study, the mechanical properties and fatigue characteristics of the two types of wheel and rail were evaluated, and the effects on wear and contact fatigue were examined. We found that the crack growth rate and the hardness were important factors in the contact fatigue and the wear. The rail steel with a higher crack growth rate and hardness had a low resistance to contact fatigue with large size damage. The hardness ratio and the total hardness are important factors in evaluating the wear resistance. In addition, we found that the residual stress increased proportionally to the maximum shear stress.
Production of Hydroxy Fatty Acids, Precursors of γ-Hexalactone, Contributes to the Characteristic Sweet Aroma of Beef
Authors: Ueda Shuji , Hosoda Mana , Kasamatsu Kumi , Horiuchi Masahiro , Nakabayashi Rio , Kang Bubwoong , Shinohara Masakazu , Nakanishi Hiroki , Ohto-Nakanishi Takayo , Yamanoue Minoru , Shirai Yasuhito
Published: April 2022

Aroma is an essential factor for meat quality. The meat of Japanese Black cattle exhibits fine marbling and a rich and sweet aroma with a characteristic lactone composition. The mechanism of lactone formation associated with beef aroma has not been elucidated. In this study, we examined the precursors of γ-hexalactone, an indicator of the sweet aroma of beef and identified the mechanism underlying γ-hexalactone production. A low-temperature vacuum system was used to prepare beef tallow from Japanese Black cattle and Holstein cattle. The odor components were identified using headspace–gas chromatography. The analysis revealed that γ-hexalactone, γ-dodecalactone, δ-tetradecalactone, and δ-hexadecalactone were present as sweet aroma components of beef tallow prepared from marbling and muscle. Since we previously reported that γ-hexalactone formation correlates with linoleic acid content in beef, we analyzed ten oxidized fatty acids derived from linoleic acid by liquid chromatography–triple quadrupole mass spectrometry and detected two hydroxy-octadecadienoic acids (9S-HODE and 13S-HODE) in beef tallow. Significant differences in arachidonic acid 15-lipoxygenase and cyclooxygenase protein expression levels among subcutaneous fat, intramuscular fat, and muscle tissue were observed. Our results suggest that the combination of linoleic acid and the expression of lipid oxidase derived from beef muscle and intramuscular fat produce hydroxy fatty acids that result in a sweet aroma.
The Effect of Surface Wettability on Viscoelastic Droplet Dynamics under Electric Fields
Authors: Wei Bo Sen , Joo Sang Woo
Published: April 2022

The effects of surface wettability and viscoelasticity on the dynamics of liquid droplets under an electric field are studied experimentally. A needle-plate electrode system is used as the power source to polarize a dielectric plate by the corona discharge emitted at the needle electrode, creating a new type of steerable electric field realized. The dynamics of droplets between the dielectric plate and a conductive substrate include three different phenomena: equilibrium to a stationary shape on substrates with higher wettability, deformation to form a bridge between the top acrylic plate and take-off on the substrates with lower wettability. Viscoelastic droplets differ from water in the liquid bridge and takeoff phenomena in that thin liquid filaments appear in viscoelastic droplets, not observed for Newtonian droplets. The equilibrated droplet exhibits more pronounced heights for Newtonian droplets compared to viscoelastic droplets, with a decrease in height with the increase in the concentration of the elastic constituent in the aqueous solution. In the take-off phenomenon, the time required for the droplet to contact the upper plate decreases with the concentration of the elastic constituent increases. It is also found that the critical voltage required for the take-off phenomenon to occur decreases as the elasticity increases.
Rational Design and Numerical Analysis of a Hybrid Floating cIDE Separator for Continuous Dielectrophoretic Separation of Microparticles at High Throughput
Authors: Li Yalin , Wang Yan , Pesch Georg R. , Baune Michael , Du Fei , Liu Xiaomin
Published: April 2022

Dielectrophoresis (DEP) enables continuous and label-free separation of (bio)microparticles with high sensitivity and selectivity, whereas the low throughput issue greatly confines its clinical application. Herein, we report a novel design of the DEP separator embedded with cylindrical interdigitated electrodes that incorporate hybrid floating electrode layout for (bio)microparticle separation at favorable throughput. To better predict microparticle trajectory in the scaled-up DEP platform, a theoretical model based on coupling of electrostatic, fluid and temperature fields is established, in which the effects of Joule heating-induced electrothermal and buoyancy flows on particles are considered. Size-based fractionation of polystyrene microspheres and dielectric properties-based isolation of MDA-MB-231 from blood cells are numerically realized, respectively, by the proposed separator with sample throughputs up to 2.6 mL/min. Notably, the induced flows can promote DEP discrimination of heterogeneous cells. This work provides a reference on tailoring design of enlarged DEP platforms for highly efficient separation of (bio)samples at high throughput.
A Biomimetic Porcine Urothelial Model for Assessing Escherichia coli Pathogenicity
Authors: Predojević Luka , Keše Darja , Žgur Bertok Darja , Železnik Ramuta Taja , Veranič Peter , Erdani Kreft Mateja , Starčič Erjavec Marjanca
Published: April 2022

Urinary tract infections can be severe, sometimes fatal, diseases whose etiological pathogens are predominantly uropathogenic strains of E. coli (UPEC). To investigate the UPEC pathogenesis, several models have already been established with minor or major disadvantages. The aim was to develop a simple, fast, and inexpensive biomimetic in vitro model based on normal porcine urothelial (NPU) cells that are genetically and physiologically similar to human bladder urothelium and to perform basic studies of E. coli pathogenicity. Initially, the model was tested using a set of control E. coli strains and, subsequently, with human E. coli strains isolated either from patients with urinary infections or from the feces of healthy individuals. A drop in viability of NPU cells was used as a measure of the pathogenicity of the individual strain tested. To visualize the subcellular events, transmission and scanning electron microscopy was performed. The strains were tested for the presence of different virulence-associated genes, phylogroup, type of core lipid, O-serotype, and type of lipopolysaccharide and a statistical analysis of possible correlations between strains’ characteristics and the effect on the model was performed. Results showed that our model has the discriminatory power to distinguish pathogenic from non-pathogenic E. coli strains, and to identify new, potentially pathogenic strains.
Three-Dimensional Gravity Inversion in the Presence of the Sediment-Basement Interface: A Case Study in Utah, USA
Authors: Zhdanov Michael S. , Jorgensen Michael , Wan Le
Published: April 2022

We introduce a novel approach to three-dimensional gravity inversion in the presence of the sediment-basement interface with a strong density contrast. This approach makes it possible to incorporate the known information about the basement depth in the inversion. It also allows the user to determine the depth-to-basement in the initial inversion phase. One can then use this interface to constrain the final inversion phase. First, the inversion generates the depth-to-basement model based on the 3D Cauchy-type integral representation of the gravity field. Then, in the second phase, full 3D voxel-type inversion applies the depth-to-basement model determined in the first phase as an a priori constraint. We use this approach to the 3D inversion of the Bouguer gravity anomaly data observed in Utah, USA. The results of inversion generated a 3D density model of the top layers of the earth’s crust, including unconsolidated sediments and the top of the crystalline basement.
Comparative Toxicity Assessment of Eco-Friendly Synthesized Superparamagnetic Iron Oxide Nanoparticles (SPIONs) in Plants and Aquatic Model Organisms
Authors: Hoffmann Nicolás , Tortella Gonzalo , Hermosilla Edward , Fincheira Paola , Diez M. Cristina , Lourenço Isabella M. , Seabra Amedea B. , Rubilar Olga
Published: April 2022

This study aimed to evaluate the toxicity of superparamagnetic iron oxide nanoparticles (SPIONs) synthesized by biogenic (BS) and chemical (CH) routes. The nanoparticles were characterized by X-ray diffraction (XRD), X-ray spectroscopy (XPS), atomic force microscopy (AFM), vibrating-sample magnetometry (VSM-SQUID), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The toxicity of SPIONs was evaluated using Artemia salina as model aquatic organisms and Raphanus sativus and Lactuca sativa as model plants to evaluate their phytotoxicity. The results obtained from XRD, XPS, and AFM confirmed the formation of spherical nanoparticles of 41.9 ± 1.00 nm (BS route) and 19.8 ± 0.47 nm (CH route). VSM-SQUID demonstrated the superparamagnetic behavior of both nanoparticles, and FT-IR provided evidence of the differences in the surface of SPIONs, suggesting the presence of phenolic compounds on the surface of BS-SPIONs. For the assays with Artemia salina, the results demonstrated (i) nonsignificant differences of BS-SPIONs in mortality rates, and (ii) significant toxicity (p < 0.05) was observed for CH-SPIONs at 300 and 400 mg L−1. The Raphanus sativa plant assay tests showed (i) BS-SPIONs and CH-SPIONs improved the root elongation of seedlings. However, BS-SPIONs demonstrated significant activity on root seedling elongation (p < 0.05) in the range of 300 mg L−1 to 600 mg L−1. To the best of our knowledge, this is the first report to compare the toxicity of chemically and biogenically synthesized SPIONs. In conclusion, although BS-SPIONs and CH-SPIONs present similar structures, their characteristics of magnetic saturation and surface structure are nonidentical, providing differences in their biological activity.
Deep-Learning-Based Automatic Mineral Grain Segmentation and Recognition
Authors: Latif Ghazanfar , Bouchard Kévin , Maitre Julien , Back Arnaud , Bédard Léo Paul
Published: April 2022

A multitude of applications in engineering, ore processing, mineral exploration, and environmental science require grain recognition and the counting of minerals. Typically, this task is performed manually with the drawback of monopolizing both time and resources. Moreover, it requires highly trained personnel with a wealth of knowledge and equipment, such as scanning electron microscopes and optical microscopes. Advances in machine learning and deep learning make it possible to envision the automation of many complex tasks in various fields of science at an accuracy equal to human performance, thereby, avoiding placing human resources into tedious and repetitive tasks, improving time efficiency, and lowering costs. Here, we develop deep-learning algorithms to automate the recognition of minerals directly from the grains captured from optical microscopes. Building upon our previous work and applying state-of-the-art technology, we modify a superpixel segmentation method to prepare data for the deep-learning algorithms. We compare two residual network architectures (ResNet 1 and ResNet 2) for the classification and identification processes. We achieve a validation accuracy of 90.5% using the ResNet 2 architecture with 47 layers. Our approach produces an effective application of deep learning to automate mineral recognition and counting from grains while also achieving a better recognition rate than reported thus far in the literature for this process and other well-known, deep-learning-based models, including AlexNet, GoogleNet, and LeNet.
Chromium-Modified Ultrathin CoFe LDH as High-Efficiency Electrode for Hydrogen Evolution Reaction
Authors: Zhang Jun-Jun , Li Meng-Yang , Li Xiang , Bao Wei-Wei , Jin Chang-Qing , Feng Xiao-Hua , Liu Ge , Yang Chun-Ming , Zhang Nan-Nan
Published: April 2022

Hydrogen evolution reaction (HER) has a dominant function in energy conversion and storage because it supplies a most effective way for converting electricity into sustainable high-purity hydrogen. Layered double hydroxides (LDHs) have shown promising performance in the process of electrochemical water oxidation (a half-reaction for water splitting). Nevertheless, HER properties have not been well released due to the structural characteristics of related materials. Herein, a simple and scalable tactics is developed to synthesize chromium-doped CoFe LDH (CoFeCr LDH). Thanks to oxygen vacancy, optimized electronic structure and interconnected array hierarchical structure, our developed ternary CoFeCr-based layered double hydroxide catalysts can provide 10 mA cm−2 current density at −0.201 V vs. RHE with superior long-term stability in alkaline electrolyte. We anticipate that the simple but feasible polymetallic electronic modulation strategy can strengthen the electrocatalytic property of the layered double hydroxides established in the present study, based on a carbon neutral and hydrogen economy.
Direct Epitaxial Growth of Polar Hf0.5Zr0.5O2 Films on Corundum
Authors: Barriuso Eduardo , Koutsogiannis Panagiotis , Serrate David , Herrero-Martín Javier , Jiménez Ricardo , Magén César , Algueró Miguel , Algarabel Pedro A. , Pardo José A.
Published: April 2022

Single-phase epitaxial Hf0.5Zr0.5O2 films with non-centrosymmetric orthorhombic structure have been grown directly on electrode-free corundum (α-Al2O3) substrates by pulsed laser deposition. A combination of high-resolution X-ray diffraction and X-ray absorption spectroscopy confirms the epitaxial growth of high-quality films belonging to the Pca21 space group, with [111] out-of-plane orientation. The surface of a 7-nm-thick sample exhibits an atomic step-terrace structure with a corrugation of the order of one atomic layer, as proved by atomic force microscopy. Scanning transmission electron microscopy reveals that it consists of grains with around 10 nm lateral size. The polar nature of this film has been corroborated by pyroelectric measurements. These results shed light on the mechanisms of the epitaxial stabilization of the ferroelectric phase of hafnia.
FIB-DIC Residual Stress Evaluation in Shot Peened VT6 Alloy Validated by X-ray Diffraction and Laser Speckle Interferometry
Authors: Somov Pavel , Statnik Eugene , Kan Yuliya , Pisarev Vladimir , Eleonsky Svyatoslav , Ozherelkov Dmitry , Salimon Alexey
Published: April 2022

Ga-ion micro-ring-core FIB-DIC evaluation of residual stresses in shot peened VT6 (Ti-6Al-4V) alloy was carried out and cross-validated against other non-destructive and semi-destructive residual stresses evaluation techniques, namely, the conventional sin2ψ X-ray diffraction and mechanical hole drilling. The Korsunsky FIB-DIC method of Ga-ion beam micro-ring-core milling within FIB-SEM with Digital Image Correlation (DIC) deformation analysis delivered spatial resolution down to a few micrometers, while the mechanical drilling of circular holes of ~2 mm diameter with laser speckle interferometry monitoring of strains gave a rough spatial resolution of a few millimeters. Good agreement was also found with the X-ray diffraction estimates of residual stress variation profiles as a function of depth. These results demonstrate that FIB-DIC provides rich information down to the micron scale, it also allows reliable estimation of macro-scale residual stresses.
A New Look at the Effects of Engineered ZnO and TiO2 Nanoparticles: Evidence from Transcriptomics Studies
Authors: Wang Shuyuan , Alenius Harri , El-Nezami Hani , Karisola Piia
Published: April 2022

Titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles (NPs) have attracted a great deal of attention due to their excellent electrical, optical, whitening, UV-adsorbing and bactericidal properties. The extensive production and utilization of these NPs increases their chances of being released into the environment and conferring unintended biological effects upon exposure. With the increasingly prevalent use of the omics technique, new data are burgeoning which provide a global view on the overall changes induced by exposures to NPs. In this review, we provide an account of the biological effects of ZnO and TiO2 NPs arising from transcriptomics in in vivo and in vitro studies. In addition to studies on humans and mice, we also describe findings on ecotoxicology-related species, such as Danio rerio (zebrafish), Caenorhabditis elegans (nematode) or Arabidopsis thaliana (thale cress). Based on evidence from transcriptomics studies, we discuss particle-induced biological effects, including cytotoxicity, developmental alterations and immune responses, that are dependent on both material-intrinsic and acquired/transformed properties. This review seeks to provide a holistic insight into the global changes induced by ZnO and TiO2 NPs pertinent to human and ecotoxicology.
Establishment of a Simple and Versatile Evaporation Compensation Model for In Vitro Chronic Ethanol Treatment: Impact on Neuronal Viability
Authors: Rath Meera , Figueroa Ariana M. , Zhang Ping , Stevens Stanley M. , Liu Bin
Published: April 2022

Alcohol overconsumption is a major cause of preventable mental disorders and death in the United States and around the world. The pathogenesis of alcohol dependence, abuse, and toxicity to the central nervous system remains incompletely understood. Cell culture-based models have been highly valuable in studying the molecular and cellular mechanisms underlying the contribution of individual CNS cell types to ethanol’s effects on the brain. However, conventional cell culture model systems carry the inherent disadvantage of rapid loss of ethanol due to evaporation following a bolus addition of ethanol at the start of the treatment. In this study, we have established a multi-well cell culture plate-based ethanol evaporation compensation model that utilizes the inter-well space as a reservoir to compensate for the evaporative loss of ethanol in the cell treatment wells. Following a single bolus addition at the start, ethanol concentration in the treatment wells rapidly decreased over time. Through compensation using the multi-well plate platform, maintenance of ethanol concentrations ranging from 10–100 mM was achieved for up to 72 h in a cell-free system. Furthermore, greater effects on ethanol-induced decrease in the viability of human dopaminergic neuronal cells were observed with than without evaporation compensation. Our method effectively compensates for the evaporative loss of ethanol typically observed in the traditional treatment method and provides a simple and economic in vitro model system for ethanol treatment over an extended timeframe where maintenance of a relatively constant concentration of ethanol is desired.
Diagnostic Accuracy of the Five-Word Test for Mild Cognitive Impairment Due to Alzheimer’s Disease
Authors: Fornari Chiara , Mori Francesco , Zoppi Nicola , Libri Ilenia , Silvestri Chiara , Cosseddu Maura , Turrone Rosanna , Maffi Matteo , Caratozzolo Salvatore , Borroni Barbara , Padovani Alessandro , Benussi Alberto
Published: April 2022

New diagnostic methods have been developed for the early diagnosis of Alzheimer’s disease (AD) with the primary purpose of intercepting the transition-phase (mild cognitive impairment, MCI) between normal aging and dementia. We aimed to explore whether the five-word test (FWT) and the mini-mental state examination (MMSE) are predictive for the early diagnosis of MCI due to AD (AD-MCI). We computed ROC analyses to evaluate the sensitivity and specificity of MMSE and FWT in predicting abnormal CSF (t-Tau, p-Tau181, Aβ1–42) and amyloid-PET biomarkers. AD-MCI patients showed lower MMSE and FWT scores (all p < 0.001) than non-AD-MCI. The best predictor of amyloid plaques’ presence at amyloid-PET imaging was the encoding sub-score of the FWT (AUC = 0.84). Both FWT and MMSE had low/moderate accuracy for the detection of pathological CSF Aβ42, t-Tau and p-Tau181 values, with higher accuracy for the t-Tau/Aβ1–42 ratio. In conclusion, the FWT, as a single-domain cognitive screening test, seems to be prompt and moderately accurate tool for the identification of an underlying AD neuropathological process in patients with MCI, supporting the importance of associating biomarkers evaluation in the work-up of patients with dementing neurodegenerative disorders.
Neophobia—A Natural Developmental Stage or Feeding Difficulties for Children?
Authors: Białek-Dratwa Agnieszka , Szczepańska Elżbieta , Szymańska Dorota , Grajek Mateusz , Krupa-Kotara Karolina , Kowalski Oskar
Published: April 2022

Food neophobia is the tendency to reject or be reluctant to try new and unfamiliar foods. Due to the period of its occurrence, which falls in the years of early childhood, it can significantly affect the child’s food choices, shape taste preferences, and significantly influence the quality of the child’s diet. The neophobic attitude has an important evolutionary significance because it protects the individual from ingesting potentially dangerous substances. On the other hand, it fosters avoidance behaviors that can also relate to the beneficial aspects of obtaining and consuming food. Currently, the strong emphasis placed on food safety means that neophobia may be less adaptive; nevertheless, a conservative attitude toward new foods still prevails. There is a strong association between food neophobia and the diversity of a person’s diet and previous exposure to different foods. This review describes behaviors associated with food neophobia and analyzes other feeding and eating difficulties in children that should be differentiated from food neophobia. Management approaches affecting the reduction in food neophobia in children through various dietary and psychological interventions are also proposed.
Effect of Long-Term Hydroxytyrosol Administration on Body Weight, Fat Mass and Urine Metabolomics: A Randomized Double-Blind Prospective Human Study
Authors: Fytili Christina , Nikou Theodora , Tentolouris Nikolaos , Tseti Ioulia K. , Dimosthenopoulos Charilaos , Sfikakis Petros P. , Simos Dimitrios , Kokkinos Alexandros , Skaltsounis Alexios L. , Katsilambros Nikolaos , Halabalaki Maria
Published: April 2022

Hydroxytyrosol (HT) is a natural antioxidant found in olive products and characterized by well-documented beneficial effects on human health. Several research studies are ongoing that aim to investigate its potency and molecular mechanism of action. The present study aimed to investigate the potential effect of HT on human obesity through a randomized double-blind prospective design. HT in two different doses (15 and 5 mg/day) and a placebo capsule was administered to 29 women with overweight/obesity for six months and their weight and fat mass were monitored at three time points (baseline, 4, 12 and 24 weeks). Statistically significant weight and visceral fat mass loss (%weight loss: p = 0.012, %visceral fat loss: p = 0.006) were observed in the group receiving the maximum HT dosage versus placebo after 4 weeks of the intervention, with attenuation of these findings at 12 and 24 weeks of the study. Urine samples were collected during the intervention and analyzed via liquid chromatography–high-resolution mass spectrometry for untargeted metabolomic purposes and comparisons between study groups were performed. HT administration was safe and well-tolerated. To the best of our knowledge, this is the first human cohort investigating the effects of HT on obesity for a prolonged study period.
CRABP1 in Non-Canonical Activities of Retinoic Acid in Health and Diseases
Authors: Nhieu Jennifer , Lin Yu-Lung , Wei Li-Na
Published: April 2022

In this review, we discuss the emerging role of Cellular Retinoic Acid Binding Protein 1 (CRABP1) as a mediator of non-canonical activities of retinoic acid (RA) and relevance to human diseases. We first discuss the role of CRABP1 in regulating MAPK activities and its implication in stem cell proliferation, cancers, adipocyte health, and neuro-immune regulation. We then discuss an additional role of CRABP1 in regulating CaMKII activities, and its implication in heart and motor neuron diseases. Through molecular and genetic studies of Crabp1 knockout (CKO) mouse and culture models, it is established that CRABP1 forms complexes with specific signaling molecules to function as RA-regulated signalsomes in a cell context-dependent manner. Gene expression data and CRABP1 gene single nucleotide polymorphisms (SNPs) of human cancer, neurodegeneration, and immune disease patients implicate the potential association of abnormality in CRABP1 with human diseases. Finally, therapeutic strategies for managing certain human diseases by targeting CRABP1 are discussed.
Sulfur Amino Acid Supplementation Abrogates Protective Effects of Caloric Restriction for Enhancing Bone Marrow Regrowth Following Ionizing Radiation
Authors: Hine Christopher , Treviño-Villarreal J. Humberto , Mejia Pedro , Longchamp Alban , Brace Lear E. , Harputlugil Eylul , Mitchell Sarah J. , Yang Jie , Guan Yihong , Maciejewski Jaroslaw P. , Jha Babal K. , Mitchell James R.
Published: April 2022

Radiation therapy damages and depletes total bone marrow (BM) cellularity, compromising safety and limiting effective dosing. Aging also strains total BM and BM hematopoietic stem and progenitor cell (HSPC) renewal and function, resulting in multi-system defects. Interventions that preserve BM and BM HSPC homeostasis thus have potential clinical significance. Here, we report that 50% calorie restriction (CR) for 7-days or fasting for 3-days prior to irradiation improved mouse BM regrowth in the days and weeks post irradiation. Specifically, one week of 50% CR ameliorated loss of total BM cellularity post irradiation compared to ad libitum-fed controls. CR-mediated BM protection was abrogated by dietary sulfur amino acid (i.e., cysteine, methionine) supplementation or pharmacological inhibition of sulfur amino acid metabolizing and hydrogen sulfide (H2S) producing enzymes. Up to 2-fold increased proliferative capacity of ex vivo-irradiated BM isolated from food restricted mice relative to control mice indicates cell autonomy of the protective effect. Pretreatment with H2S in vitro was sufficient to preserve proliferative capacity by over 50% compared to non-treated cells in ex vivo-irradiated BM and BM HSPCs. The exogenous addition of H2S inhibited Ten eleven translocation 2 (TET2) activity in vitro, thus providing a potential mechanism of action. Short-term CR or fasting therefore offers BM radioprotection and promotes regrowth in part via altered sulfur amino acid metabolism and H2S generation, with translational implications for radiation treatment and aging.
Macronutrients and Micronutrients in Parenteral Nutrition for Preterm Newborns: A Narrative Review
Authors: Rizzo Valentina , Capozza Manuela , Panza Raffaella , Laforgia Nicola , Baldassarre Maria Elisabetta
Published: April 2022

Preterm neonates display a high risk of postnatal malnutrition, especially at very low gestational ages, because nutritional stores are less in younger preterm infants. For this reason nutrition and growth in early life play a pivotal role in the establishment of the long-term health of premature infants. Nutritional care for preterm neonates remains a challenge in clinical practice. According to the recent and latest recommendations from ESPGHAN, at birth, water intake of 70–80 mL/kg/day is suggested, progressively increasing to 150 mL/kg/day by the end of the first week of life, along with a calorie intake of 120 kcal/kg/day and a minimum protein intake of 2.5–3 g/kg/day. Regarding glucose intake, an infusion rate of 3–5 mg/kg/min is recommended, but VLBW and ELBW preterm neonates may require up to 12 mg/kg/min. In preterm infants, lipid emulsions can be started immediately after birth at a dosage of 0.5–1 g/kg/day. However, some authors have recently shown that it is not always possible to achieve optimal and recommended nutrition, due to the complexity of the daily management of premature infants, especially if extremely preterm. It would be desirable if multicenter randomized controlled trials were designed to explore the effect of early nutrition and growth on long-term health.
Healthy Food Pyramid as Well as Physical and Mental Activity in the Prevention of Alzheimer’s Disease
Authors: Kępka Alina , Ochocińska Agnieszka , Borzym-Kluczyk Małgorzata , Chojnowska Sylwia , Skorupa Ewa , Przychodzeń Małgorzata , Waszkiewicz Napoleon
Published: April 2022

The ageing of the population is resulting in neurodegenerative diseases, including Alzheimer’s disease (AD), which are an increasing social, economic and medical problem. Diet and physical activity are now considered as important modifiable factors that help prevent or delay the development of AD and other dementia-related diseases. The pyramid of healthy nutrition and lifestyle is a way of presenting the principles, the implementation of which gives a chance for proper development and a long healthy life. The basis of the pyramid, in the first place, is physical activity. Our review of the literature in the PubMed database supports the hypothesis that complementary factors, such as proper diet, physical exercise and mental activity, have a positive impact on the prevention of neurodegenerative diseases. The nutritional recommendations for healthy adults primarily include the consumption of vegetables, fruits, cereals, legumes, vegetable oils and fishes. Therefore, the introduction of Mediterranean and Asian diets may reduce the risk of the neurodegenerative diseases associated with dementia, whereas dairy products and meat—the main sources of L-carnitine—should be consumed in moderate amounts. The aim of our work is to provide up-to-date knowledge about the appropriate dietary model and healthy lifestyle elements and their impact on good health and the long life of people.