bluuebunny/crossref_metadata_2025 huggingface dataset:
CaMKIIα neurons in the anterior insular cortex regulate attention behavior in mice
Authors: Ma Yingping , Jiang Shaofei , Zhao Xin , Li Shen , Chen Liping , Zhao Zhe , Shen Wei , Wu Yan , Wu Haitao
Published: July 2023

Introduction

The insular cortex is involved in multiple physiological processes including working memory, pain, emotion, and interoceptive functions. Previous studies have indicated that the anterior insular cortex (aIC) also mediates interoceptive attention in humans. However, the exact cellular and physiological function of the aIC in the regulation of this process is still elusive.

Methods

In this study, using the 5-choice serial reaction time task (5-CSRTT) testing paradigm, we assessed the role of the aIC in visuospatial attention and impulsiveness in mice.

Results

The results showed a dramatic activation of c-Fos in the aIC CaMKIIα neurons after the 5-CSRTT procedure. In vivo fiber photometry revealed enhanced calcium signaling in aIC CaMKIIα neurons when the mice responded correctly. In addition, chemogenetic suppression of aIC CaMKIIα neurons led to increased incorrect responses within the appropriate time. Importantly, pharmacological activation of aIC CaMKIIα neurons enhanced their performance in the 5-CSRTT test.

Discussion

These results provide compelling evidence that aIC CaMKIIα neurons are essential for the modulation of attentional processing in mice.
A review of invasive intracranial pressure monitoring following surgery for hypertensive cerebral hemorrhage
Authors: Chen Fu , Zhang Shukui , Li Bingzhen , Zhang Jin , Ran Maoxin , Qi Bin
Published: July 2023

Hypertensive cerebral hemorrhage, the most common prevalent of spontaneous cerebral hemorrhage, poses a significant threat to patient mortality and morbidity, while therapeutic options remain limited, making the disease a burden not only for patients’ families but also a major challenge for national healthcare systems. The elevation of intracranial pressure subsequent to hypertensive cerebral hemorrhage is a critical contributor to mortality. However, it often manifests before the onset of clinical symptoms, which are typically atypical, leading to delayed treatment and irreversible consequences for the patient. Hence, early detection of intracranial pressure variations can aid in timely, efficient, and precise treatment, reducing patient mortality. Invasive intracranial pressure monitoring enables real-time, accurate monitoring of intracranial pressure changes, providing clinicians with therapeutic guidance and overcoming the limitations of empirical treatment. This article aims to review the use of invasive intracranial pressure monitoring in postoperative hypertensive cerebral hemorrhage and hopes to contribute to clinical and scientific research.
Intracranial hemorrhage detection in 3D computed tomography images using a bi-directional long short-term memory network-based modified genetic algorithm
Authors: Sengupta Jewel , Alzbutas Robertas , Falkowski-Gilski Przemysław , Falkowska-Gilska Bożena
Published: July 2023

Introduction

Intracranial hemorrhage detection in 3D Computed Tomography (CT) brain images has gained more attention in the research community. The major issue to deal with the 3D CT brain images is scarce and hard to obtain the labelled data with better recognition results.

Methods

To overcome the aforementioned problem, a new model has been implemented in this research manuscript. After acquiring the images from the Radiological Society of North America (RSNA) 2019 database, the region of interest (RoI) was segmented by employing Otsu’s thresholding method. Then, feature extraction was performed utilizing Tamura features: directionality, contrast, coarseness, and Gradient Local Ternary Pattern (GLTP) descriptors to extract vectors from the segmented RoI regions. The extracted vectors were dimensionally reduced by proposing a modified genetic algorithm, where the infinite feature selection technique was incorporated with the conventional genetic algorithm to further reduce the redundancy within the regularized vectors. The selected optimal vectors were finally fed to the Bi-directional Long Short Term Memory (Bi-LSTM) network to classify intracranial hemorrhage sub-types, such as subdural, intraparenchymal, subarachnoid, epidural, and intraventricular.

Results

The experimental investigation demonstrated that the Bi-LSTM based modified genetic algorithm obtained 99.40% sensitivity, 99.80% accuracy, and 99.48% specificity, which are higher compared to the existing machine learning models: Naïve Bayes, Random Forest, Support Vector Machine (SVM), Recurrent Neural Network (RNN), and Long Short-Term Memory (LSTM) network.
Opportunities and barriers for maternal nutrition behavior change: an in-depth qualitative analysis of pregnant women and their families in Uttar Pradesh, India
Authors: Jhaveri Neha R. , Poveda Natalia E. , Kachwaha Shivani , Comeau Dawn L. , Nguyen Phuong H. , Young Melissa F.
Published: July 2023

Background

Maternal undernutrition during pregnancy remains a critical public health issue in India. While evidence-based interventions exist, poor program implementation and limited uptake of behavior change interventions make addressing undernutrition complex. To address this challenge, Alive & Thrive implemented interventions to strengthen interpersonal counseling, micronutrient supplement provision, and community mobilization through the government antenatal care (ANC) platform in Uttar Pradesh, India.

Objective

This qualitative study aimed to: (1) examine pregnant women’s experiences of key nutrition-related behaviors (ANC attendance, consuming a diverse diet, supplement intake, weight gain monitoring, and breastfeeding intentions); (2) examine the influence of family members on these behaviors; and (3) identify key facilitators and barriers that affect behavioral adoption.

Methods

We conducted a qualitative study with in-depth interviews with 24 pregnant women, 13 husbands, and 15 mothers-in-law (MIL). We analyzed data through a thematic approach using the Capability-Opportunity-Motivation-Behavior (COM-B) framework.

Results

For ANC checkups and maternal weight gain monitoring, key facilitators were frontline worker home visits, convenient transportation, and family support, while the primary barrier was low motivation and lack understanding of the importance of ANC checkups. For dietary diversity, there was high reported capability (knowledge related to the key behavior) and most family members were aware of key recommendations; however, structural opportunity barriers (financial strain, lack of food availability and accessibility) prevented behavioral change. Opportunity ranked high for iron and folic acid supplement (IFA) intake, but was not consistently consumed due to side effects. Conversely, lack of supply was the largest barrier for calcium supplement intake. For breastfeeding, there was low overall capability and several participants described receiving inaccurate counseling messages.

Conclusion

Key drivers of maternal nutrition behavior adoption were indicator specific and varied across the capability-opportunity-motivation behavior change spectrum. Findings from this study can help to strengthen future program effectiveness by identifying specific areas of program improvement.
Camrelizumab as a novel third or post-third-line treatment strategy in small cell lung cancer: a retrospective study of 12 patients
Authors: Tian Jizheng , Sui Lili , Wang Hong , Chen Xiaoyan
Published: July 2023

Background

Small cell lung cancer (SCLC) constitutes 15% of all lung cancer cases, with a comparatively low survival rate. The advent of immune checkpoint inhibitors (ICIs) has provided new alternatives for treating SCLC. However, the effectiveness of camrelizumab in the treatment of SCLC remains unclear. This retrospective case series was designed to investigate the efficacy and safety of camrelizumab in SCLC patients.

Methods

The study enrolled SCLC patients recorded as having received more than one cycle of camrelizumab in the electronic medical record system. Data related to clinical and survival times were collected and statistically analyzed.

Results

From August 2019 to December 2021, the study enrolled 12 SCLC patients. The objective response rate was 41.7% (95% confidence interval [CI]: 15.2%–72.3%). The disease control rate was 83.3% (95% CI: 51.6%–97.9%). The median progression-free survival (PFS) for all patients was 4.0 months. Notably, the median PFS of patients in third- or post-third-line subgroups was 7 months (95% CI: 1.12–12.88 months). The median overall survival (OS) for all eligible patients was 10.0 months (95% CI: 7.35–12.65 months), with a 1-year survival rate of 25%. Notably, the OS of patients treated with third- or post-third-line therapy was 5–34 months, with a 1-year survival rate of 75%. The two most prevalent non-hematological adverse events associated with the immune response were pneumonitis (44.4%) and reactive cutaneous capillary endothelial proliferation (44.4%). One patient experienced an exacerbation of preexisting diabetes and reached grade 3 hyperglycemia. There were no grade 4/5 immune-related adverse events.

Conclusion

This case series highlights the potential benefits and safety concerns of camrelizumab in SCLC patients. These findings suggest a possible strategy for third- and post-third-line treatments of SCLC. However, the conclusion is limited due to the study’s retrospective nature and small sample size. Therefore, large-scale randomized controlled studies are needed to determine its efficacy.
Spirometric and anthropometric improvements in response to elexacaftor/tezacaftor/ivacaftor depending on age and lung disease severity
Authors: Schütz Katharina , Pallenberg Sophia Theres , Kontsendorn Julia , DeLuca David , Sukdolak Cinja , Minso Rebecca , Büttner Tina , Wetzke Martin , Dopfer Christian , Sauer-Heilborn Annette , Ringshausen Felix C. , Junge Sibylle , Tümmler Burkhard , Hansen Gesine , Dittrich Anna-Maria
Published: July 2023

Introduction: Triple-combination cystic fibrosis transmembrane conductance regulator (CFTR) modulator therapy with elexacaftor/tezacaftor/ivacaftor (ETI) was introduced in August 2020 in Germany for people with CF (pwCF) ≥12 years (yrs.) of age and in June 2021 for pwCF ≥6 yrs of age. In this single-center study, we analyzed longitudinal data on the percent-predicted forced expiratory volume (ppFEV1) and body-mass-index (BMI) for 12 months (mo.) after initiation of ETI by linear mixed models and regression analyses to identify age- and severity-dependent determinants of response to ETI.

Methods: We obtained data on 42 children ≥6–11 yrs, 41 adolescents ≥12–17 yrs, and 143 adults by spirometry and anthropometry prior to ETI, and 3 and 12 mo. after ETI initiation. Data were stratified by the age group and further sub-divided into age-specific ppFEV1 impairment. To achieve this, the age strata were divided into three groups, each according to their baseline ppFEV1: lowest 25%, middle 50%, and top 25% of ppFEV1.

Results: Adolescents and children with more severe lung disease prior to ETI (within the lowest 25% of age-specific ppFEV1) showed higher improvements in lung function than adults in this severity group (+18.5 vs. +7.5; p = 0.002 after 3 mo. and +13.8 vs. +7.2; p = 0.012 after 12 mo. of ETI therapy for ≥12–17 years and +19.8 vs. +7.5; p = 0.007 after 3 mo. for children ≥6–11 yrs). In all age groups, participants with more severe lung disease showed higher BMI gains than those with medium or good lung function (within the middle 50% or top 25% of age-specific ppFEV1). Regression analyses identified age as a predictive factor for FEV1 increase at 3 mo. after ETI initiation, and age and ppFEV1 at ETI initiation as predictive factors for FEV1 increase 12 mo. after ETI initiation.

Discussion: We report initial data, which suggest that clinical response toward ETI depends on age and lung disease severity prior to ETI initiation, which argue for early initiation of ETI.
Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell
Authors: Wu Wenbin , Xia Qingsong , Guo Yujin , Wang Hongzhan , Dong Hui , Lu Fuer , Yuan Fen
Published: July 2023

Background: The evidence on berberine stimulating the secretion of GLP-1 in intestinal L cell has been studied. However, few research has explored its role on generating GLP-1 of islet α cell. Our experiment aims to clarify the mechanism of berberine promoting the secretion of GLP-1 in intestinal L cell and islet α cell, activating GLP-1R and its downstream molecules through endocrine and paracrine ways, thus improving the function of islet β cell and treating T2DM.

Methods: After confirming that berberine can lower blood glucose and improve insulin resistance in db/db mice, the identity maintenance, proliferation and apoptosis of islet cells were detected by immunohistochemistry and immunofluorescence. Then, the activation of berberine on GLP-1/GLP-1R/PKA signaling pathway was evaluated by Elisa, Western blot and PCR. Finally, this mechanism was verified by in vitro experiments on Min6 cells, STC-1 cells and aTC1/6 cells.

Results: Berberine ameliorates glucose metabolism in db/db mice. Additionally, it also increases the number and enhances the function of islet β cell. This process is closely related to improve the secretion of intestinal L cell and islet α cell, activate GLP-1R/PKA signaling pathway through autocrine and paracrine, and increase the expression of its related molecule such as GLP-1, GLP-1R, PC1/3, PC2, PKA, Pdx1. In vitro, the phenomenon that berberine enhanced the GLP-1/GLP-1R/PKA signal pathway had also been observed, which confirmed the results of animal experiments.

Conclusion: Berberine can maintain the identity and normal function of islet β cell, and its mechanism is related to the activation of GLP-1/GLP-1R/PKA signal pathway in intestinal L cell and islet α cell.
A comparative study of Chinese women 3 × 3 basketball players exercise load in Tokyo Olympic preparation cycle
Authors: Wang Zhe , Cao Guohuan , Xu Jiamin , Qiu Jun , Yang Ruoyu
Published: July 2023

Objective: The aim of this study was to compare the variances in-game loads exhibited by Chinese women’s 3 × 3 basketball team across different stages of the preparation cycle for the Tokyo Olympic Games, and to summarize the fundamental regulations governing Chinese women’s 3 × 3 basketball training and games, in order to establish a theoretical research foundation for the team’s new preparation cycle.

Methods: This study measured load-related data during the preparation and main competition periods of the 2019–2021 Tokyo Olympics, from April to August 2019 and from April to June 2021. The aim was to compare the changes and differences in a load of competition during different stages and to explore patterns of load changes during the preparation period. This study used wearable devices authorized by FIFA and NBA, along with the Catapult GPS performance monitoring system from Australia (Catapult&Polar Team) as instruments for collecting sports load data. The OptimEye S5 device was worn around the athlete’s neck to collect data prior to the game, while the Open Field™ system was utilized for data editing and report generation post-game.

Results: Compared to the primary competition load during the 2019 preparation period, the 2021 preparation period exhibited significant increases and decreases (p < 0.05) in competition load, high-intensity load, the number of explosive moves, the number of high-intensity acceleration, several changes to the left and right, and the number of explosive jumps. During the 2021 preparation period, the mean heart rate, mean heart rate percentage, and mean speed of the race demonstrated significant decreases in comparison to the race during the 2019 preparation period (p < 0.05). Throughout the training period spanning from 2019 to 2021, no significant differences were observed in running distance and maximum speed (p > 0.05).

Conclusion: The findings of this study reveal that the national training team has fostered positive adaptive changes in athletes, resulting in a significant enhancement in both load and sports performance science data during competition from 2019 to 2021.
The pathogenic role of intestinal flora metabolites in diabetic nephropathy
Authors: Tian En , Wang Feng , Zhao Lei , Sun Yan , Yang Jurong
Published: July 2023

With the increasing incidence of diabetes, diabetic kidney disease has become a major cause of chronic kidney disease. The role of the gut microbiota in diabetes and its related complications have been extensively investigated; the modulatory effect of the gut microbiota on the host depends on several gut microbial metabolites, particularly short-chain fatty acids, secondary bile acids, and trimethylamine N-oxide. In this review, we focused on the evidence related to the pathogenic role of each of the gut microbial metabolites in diabetic nephropathy. The main novel therapies targeting the gut microbiota include probiotics, dietary prebiotics, synbiotic supplements, and faecal microbiota transplants, although there is no standard treatment principle. Further research is therefore needed to elucidate the link between gut microbes and diabetic nephropathy, and more therapeutic targets should be explored to treat diabetic nephropathy with dysbiosis of the gut microbes.
Epidemiology of flavescence dorée and hazelnut decline in Slovenia: geographical distribution and genetic diversity of the associated 16SrV phytoplasmas
Authors: Kogej Zwitter Zala , Seljak Gabrijel , Jakomin Tjaša , Brodarič Jakob , Vučurović Ana , Pedemay Sandra , Salar Pascal , Malembic-Maher Sylvie , Foissac Xavier , Mehle Nataša
Published: July 2023

Flavescence dorée (FD) phytoplasma from 16SrV-C and -D subgroups cause severe damage to grapevines throughout Europe. This phytoplasma is transmitted from grapevine to grapevine by the sap-sucking leafhopper Scaphoideus titanus. European black alder and clematis serve as perennial plant reservoirs for 16SrV-C phytoplasma strains, and their host range has recently been extended to hazelnuts. In Slovenia, hazelnut orchards are declining due to 16SrV phytoplasma infections, where large populations of the non-autochthonous leafhopper Orientus ishidae have been observed. To better characterise the phytoplasma-induced decline of hazelnut and possible transmission fluxes between these orchards and grapevine, genetic diversity of 16SrV phytoplasmas in grapevine, hazelnut and leafhoppers was monitored from 2017 to 2022. The nucleotide sequence analysis was based on the map gene. The most prevalent map genotype in grapevine in all wine-growing regions of Slovenia was M54, which accounted for 84% of the 176 grapevines tested. Besides M54, other epidemic genotypes with lower frequency were M38 (6%), M51 (3%), M50 (2%) and M122 (1%). M38, M50 and M122 were also detected in infected cultivated hazelnuts and in specimens of O. ishidae leafhopper caught in declining hazelnut orchards. It suggests that this polyphagous vector could be responsible for phytoplasma infection in hazelnut orchards and possibly for some phytoplasma exchanges between hazelnuts and grapevine. We hereby describe new genotypes: M158 in grapevine as well as four never reported genotypes M159 to M162 in hazelnut. Of these four genotypes in hazelnut, one (M160) was also detected in O. ishidae. Analysis of additional genes of the new genotypes allowed us to assign them to the VmpA-III cluster, which corresponds to the 16SrV-C strains previously shown to be compatible with S. titanus transmission.
An upgraded method of high-throughput chromosome conformation capture (Hi-C 3.0) in cotton (Gossypium spp.)
Authors: Han Jin , Wang Siyuan , Wu Hongyu , Zhao Ting , Guan Xueying , Fang Lei
Published: July 2023

High-throughput chromosome conformation capture (Hi-C) technology has been applied to explore the chromatin interactions and shed light on the biological functions of three-dimensional genomic features. However, it remains challenging to guarantee the high quality of Hi-C library in plants and hence the reliable capture of chromatin structures, especially loops, due to insufficient fragmentation and low efficiency of proximity ligations. To overcome these deficiencies, we optimized the parameters of the Hi-C protocol, principally the cross-linking agents and endonuclease fragmentation strategy. The double cross-linkers (FA+DSG) and double restriction enzymes (DpnII+DdeI) were utilized. Thus, a systematic in situ Hi-C protocol was designed using plant tissues embedded with comprehensive quality controls to monitor the library construction. This upgraded method, termed Hi-C 3.0, was applied to cotton leaves for trial. In comparison with the conventional Hi-C 2.0, Hi-C 3.0 can obtain more than 50% valid contacts at a given sequencing depth to improve the signal-to-noise ratio. Hi-C 3.0 can furthermore enhance the capturing of loops almost as twice as that of Hi-C 2.0. In addition, Hi-C 3.0 showed higher efficiency of compartment detection and identified compartmentalization more accurately. In general, Hi-C 3.0 contributes to the advancement of the Hi-C method in plants by promoting its capability on decoding the chromatin organization.
Motivation of sex workers who provide camming services to engage in sex with their real-life and virtual partners
Authors: Kelberga (Kelberg) Anna , Martinsone Baiba
Published: July 2023

In this study, the motivations of 80 sex workers who provide camming services (76 females and 4 non-binary/trans/gender fluid individuals; aged from 20 to 49 years, M = 30.68, SD = 6.43; 56.2% married or in a committed relationship, 18.8% in a non-committed relationship and 25% - single) were compared in terms of engaging in sexual activity with their real-life partners versus their virtual partners (predominantly kink-oriented clients). Presented with 16 reasons to engage in sexual activity, the respondents rated the frequency to engage in sex for each of these reasons with their real-life and virtual partners. Results showed that there were five reasons in which there were differences in motivation to engage in sex with real-life versus virtual partners and 11 reasons showed no differences. Specifically, respondents reported engaging in sex more often with their virtual partners to get resources and to experience a specific type of sex (kink), while they reported engaging in sex more often with their real-life partners to experience physical pleasure, motivated by physical desirability of a partner and to express love and commitment. However, for all other reasons that motivate people to engage in sex, including stress reduction, experience seeking, self-esteem boost, social status, revenge, utilitarian reasons, emotional expression, duty or pressure, thrill of the forbidden, mate guarding, and desire to have sex with a person of other gender, respondents engaged in sex equally frequently with their real-life and virtual partners (clients) and there were no statistical differences. This study adds to the existing research on sex work by providing insights into the motivations of sex workers to engage in sex with different partners and demonstrates that apart from common sense differences the reasons to engage in sex with clients and real-life partners are vastly similar.
The emotional intelligence of today’s parents – influences on parenting style and parental competence
Authors: Șițoiu Andreea , Pânișoară Georgeta
Published: July 2023

The emotional intelligence of parents and parental competence become two premises of effective parenting. This study contributes to the understanding of how parents’ emotional intelligence exerts its effect on both their parenting style and parenting competence. The present research also sought to identify the factors that determine the level of parental competence. The research method used is the questionnaire-based survey. The research sample, represented by 610 respondents, was determined by the sampling strategy based on convenience, respectively by the snowball method. The results indicated that the emotional intelligence of parents is associated with an increased level of parental competence (r = 0.24,p < 0.001), and 15% of the variability of parental competence is determined by the level of parental emotional intelligence (R = 0.38,p < 0.00, R2 = 0.15). It was demonstrated that 6% of the variability of parental competence is determined by the level of parents’ self-esteem (R = 0.24,p < 0.001, R2 = 0.06). The emotional intelligence and self-esteem of the parents contribute to the development of a high level of parental competence, while the level of education of the respondents according to this research partially influences the development of parental competence. Despite the fact that a high educational level of parents is assumed to give them access to quality information, the Barnum effect is experienced regardless of educational level. According to the field of parenting, the Barnum effect refers to consulting non-scientifically validated sources and trusting information that responds to parents’ wishes, but not to children’s educational needs.These results highlight the role of parents’ emotional intelligence on the growth and education of children, but also the usefulness of training programs in the field of parenting with the aim of training parents in the necessary skills for effective parenting.
Ontological how and why: action and objective of planned processes in the food domain
Authors: Dooley Damion , Naravane Tarini
Published: July 2023

The computational modeling of food processing, aimed at various applications including industrial automation, robotics, food safety, preservation, energy conservation, and recipe nutrition estimation, has been ongoing for decades within food science research labs, industry, and regulatory agencies. The datasets from this prior work have the potential to advance the field of data-driven modeling if they can be harmonized, but this requires a standardized language as a starting point. Our primary goal is to explore two interdependent aspects of this language: the granularity of process modeling sub-parts and parameter details and the substitution of compatible inputs and processes. A delicate semantic distinction—categorizing planned processes based on the objectives they seek to fulfill vs. categorizing them by the actions or mechanisms they utilize—helps organize and facilitate this endeavor. To bring an ontological lens to process modeling, we employ the Open Biological and Biomedical Ontology Foundry ontological framework to organize two main classes of the FoodOn upper-level material processing hierarchy according to objective and mechanism, respectively. We include examples of material processing by mechanism, ranging from abstract ones such as “application of energy” down to specific classes such as “heating by microwave.” Similarly, material processing by objective—often a transformation to bring about materials with certain qualities or composition—can, for example, range from “material processing by heating threshold” to “steaming rice”.
Detection of exon 5 c.577del variant of human erythropoietin gene in whole blood, dried blood spots and urine samples for doping control
Authors: Donati Francesco , Concetti Livia , de la Torre Xavier , Zhou Xinmiao , Zhang Lisi , Botrè Francesco
Published: July 2023

Background: In doping control, the presence of the exon5 c.577del variant in the human erythropoietin gene may be a confounding factor in the interpretation of the results from the analytical method currently in force for the detection of human recombinant erythropoietin, based on immunoelectrophoresis on SDS-PAGE and/or SAR-PAGE. This variant, determining the transcription of a higher molecular weight protein, can erroneously suggest the presence of recombinant erythropoietin in a biological sample, causing the possibility of a false positive result. Although the variant was now identified only in East Asian populations and with a very low frequency, it can threaten the reliability of current anti-doping tests.

Methods: We have implemented a genetic test to identify the presence of this variant in the biological samples that are presently collected for anti-doping analysis (whole blood, urine, and dried blood spots). The test is based on the Sanger sequencing of the human erythropoietin gene exon 5, where the c.577del variant falls.

Results and Discussion: The method has a specificity of 100% and allows identification of the possible presence of the variant starting from 100 pg of genomic DNA extracted from each biological sample. The efficacy of the test has been confirmed by the analysis of real samples from subjects showing and not showing the exon c.577del variant.
“It probably could've done with less plastic” - Consumers' cyclical and uneasy relationship with food packaging
Authors: Ruippo Lotta , Kylkilahti Eliisa , Sekki Sanna , Autio Minna
Published: July 2023

Food packaging has an essential function in the contemporary food supply chain, but it is also a key source of municipal solid waste. The ability to package foods has changed eating habits as takeaway coffees, bottled water, and fast food have become more commonplace. Although the task of recycling packaging materials falls on the consumer who is guided to sort the waste and ensure it is taken to a recycling bin, the consumer perspectives of the mutually constitutive market device–consumer relationship are not yet well-known. This paper studies how food shoppers are constructing their relationship with packaging in their everyday lives, and especially how their moral considerations construct the relationship with sustainability and materiality of packaging. Based on the analysis of consumer interviews, the study argues that consumers' perspective on packaging use is renegotiated during their continuous relationship with packaging. Food packaging acts as a political market device that evokes morally charged consumer perspectives throughout different stages of consumption processes beyond the supermarket. In the first stage, the consumer is mainly focused on finding the products that have already become a part of their daily routine and the materiality of packaging oftentimes remains unseen. Tensions arise as packaging is both a source of frustration, and a necessary element of managing food consumption. After eating the food product, the packaging turns into waste and the consumer “becomes aware” of the packaging materials and several negative interpretations arise. Finally, packaging waste becomes morally charged: it invites consumers to partake in recycling work and evokes environmental anxieties. The results indicate that consumers often have an uneasy, cyclical relationship with packaging use.
Exploring the determinants of organic matter bioavailability through substrate-explicit thermodynamic modeling
Authors: Ahamed Firnaaz , You Yaqi , Burgin Amy , Stegen James C. , Scheibe Timothy D. , Song Hyun-Seob
Published: July 2023

Microbial decomposition of organic matter (OM) in river corridors is a major driver of nutrient and energy cycles in natural ecosystems. Recent advances in omics technologies enabled high-throughput generation of molecular data that could be used to inform biogeochemical models. With ultrahigh-resolution OM data becoming more readily available, in particular, the substrate-explicit thermodynamic modeling (SXTM) has emerged as a promising approach due to its ability to predict OM degradation and respiration rates from chemical formulae of compounds. This model implicitly assumes that all detected organic compounds are bioavailable, and that aerobic respiration is driven solely by thermodynamics. Despite promising demonstrations in previous studies, these assumptions may not be universally valid because OM degradation is a complex process governed by multiple factors. To identify key drivers of OM respiration, we performed a comprehensive analysis of diverse river systems using Fourier-transform ion cyclotron resonance mass spectrometry OM data and associated respiration measurements collected by the Worldwide Hydrobiogeochemistry Observation Network for Dynamic River Systems (WHONDRS) consortium. In support of our argument, we found that the incorporation of all compounds detected in the samples into the SXTM resulted in a poor correlation between the predicted and measured respiration rates. The data-model consistency was significantly improved by the selective use of a small subset (i.e., only about 5%) of organic compounds identified using an optimization method. Through a subsequent comparative analysis of the subset of compounds (which we presume as bioavailable) against the full set of compounds, we identified three major traits that potentially determine OM bioavailability, including: (1) thermodynamic favorability of aerobic respiration, (2) the number of C atoms contained in compounds, and (2) carbon/nitrogen (C/N) ratio. We found that all three factors serve as “filters” in that the compounds with undesirable properties in any of these traits are strictly excluded from the bioavailable fraction. This work highlights the importance of accounting for the complex interplay among multiple key traits to increase the predictive power of biogeochemical and ecosystem models.
Pathologic complete response to TNT + camrelizumab for rectal cancer with surgical anus-preservation: case report and literature review
Authors: Pi Feng , Tang Gang , Xie Chaozheng , Cao Yukun , Yang Shilai , Wei Zhengqiang
Published: July 2023

Background

This case report demonstrates the efficacy of total neoadjuvant therapy (TNT) based on pathological complete response (PCR). We also discuss the surgical approach to preserving the anus and its perioperative management.

Case presentaion

The patient was a 26-year-old woman, with blood in the stool and stool thinning for over two months. Preoperative examination revealed locally advanced rectal cancer invading the left anal raphe and enlarged lymph nodes adjacent to the left internal iliac vessels. The lesion was preoperatively classified as T4bN1bM0 IIIC. Considering the size and depth of the tumor, it was difficult to have sufficient margins for radical resection, and the tumor was too close to the anal orifice. Considering the patient's youth and strong desire to preserve the anus, it was decided to use TNT combined with a camrelizumab regimen. After the entire course of neoadjuvant radiotherapy, the tumor size significantly reduced in fibrotic manifestations, and no enlargement of the lymph nodes adjacent to the left internal iliac vessels was observed. She underwent robotic laparoscopic ultra-low anterior rectal resection, left lateral lymph node dissection, and temporary ileostomy, and no significant residue was observed after all bowel tubes were taken for examination, nor was there cancerous involvement at the distal or radial cut edges, or metastasis. The patient was discharged nine days postoperatively, and no major complications were detected. Follow-up was performed without adjuvant chemotherapy.

Conclusions

TNT may be a better surgical option for preserving the anus and for complete radical resection in patients with LARC for whom Miles’ resection is indicated.
Veterinarian burnout demographics and organizational impacts: a narrative review
Authors: Steffey Michele A. , Griffon Dominique J. , Risselada Marije , Scharf Valery F. , Buote Nicole J. , Zamprogno Helia , Winter Alexandra L.
Published: July 2023

Burnout is a work-related syndrome of physical and emotional exhaustion secondary to prolonged, unresolvable occupational stress. Individuals of different demographic cohorts may have disparate experiences of workplace stressors and burnout impacts. Healthcare organizations are adversely affected by burnt out workers through decreased productivity, low morale, suboptimal teamwork, and potential impacts on the quality of patient care. In this second of two companion reviews, the demographics of veterinary burnout and the impacts of burnout on affected individuals and work environments are summarized, before discussing mitigation concepts and their extrapolation for targeted strategies within the veterinary workplace and profession.
Spectral Visualization of Alloy Reactions during Laser Melting
Authors: Volpp Joerg , Naesstroem Himani , Wockenfuss Lisanne , Schmidt Malte , Partes Knut
Published: July 2023

Laser materials processing includes rapid heating to possibly high temperatures and rapid cooling of the illuminated materials. The material reactions can show significant deviations from equilibrium processing. During processing of complex materials and material combinations, it is mainly unknown how the materials react and mix. However, it is important to know which chemical elements or compounds are present in the material to define the alloy. In addition, their distribution after rapid cooling needs to be better understood. Therefore, such alloy changes at rapid heating induced by laser illumination were created as pre-placed and pre-mixed powder nuggets. The energy input and the material ratio between the powder components were varied to identify characteristic responses. For the detection of reaction durations and mixing characteristics, the vapor plume content was assumed to contain the necessary information. Spectral measurements of the plume were used to identify indicators about process behaviors. It was seen that the spectral data give indications about the chemical reactions in the melt pool. The reactions of iron ore components with aluminum seem to require laser illumination to finish completely, although the thermite reaction should maintain the chemical reaction, likely due to the required melt mixing that enables the interaction of the reacting partners at all.
Fine-Tuning through Generations: Advances in Structure and Production of CAR-T Therapy
Authors: Zheng Zhibo , Li Siyuan , Liu Mohan , Chen Chuyan , Zhang Lu , Zhou Daobin
Published: July 2023

Chimeric antigen receptor (CAR)-T cell therapy is a promising form of immunotherapy that has seen significant advancements in the past few decades. It involves genetically modifying T cells to target cancer cells expressing specific antigens, providing a novel approach to treating various types of cancer. However, the initial success of first-generation CAR-T cells was limited due to inadequate proliferation and undesirable outcomes. Nonetheless, significant progress has been made in CAR-T cell engineering, leading to the development of the latest fifth-generation CAR-T cells that can target multiple antigens and overcome individual limitations. Despite these advancements, some shortcomings prevent the widespread use of CAR-T therapy, including life-threatening toxicities, T-cell exhaustion, and inadequate infiltration for solid tumors. Researchers have made considerable efforts to address these issues by developing new strategies for improving CAR-T cell function and reducing toxicities. This review provides an overview of the path of CAR-T cell development and highlights some of the prominent advances in its structure and manufacturing process, which include the strategies to improve antigen recognition, enhance T-cell activation and persistence, and overcome immune escape. Finally, the review briefly covers other immune cells for cancer therapy and ends with the discussion on the broad prospects of CAR-T in the treatment of various diseases, not just hematological tumors, and the challenges that need to be addressed for the widespread clinical application of CAR-T cell therapies.
Comparative In Silico Analysis of Ultra-Hypofractionated Intensity-Modulated Photon Radiotherapy (IMRT) Versus Intensity-Modulated Proton Therapy (IMPT) in the Pre-Operative Treatment of Retroperitoneal Sarcoma
Authors: Gogineni Emile , Chen Hao , Istl Alexandra C. , Johnston Fabian M. , Narang Amol , Deville Curtiland
Published: July 2023

Background: While pre-operative radiation did not improve abdominal recurrence-free survival for retroperitoneal sarcoma (RPS) in the randomized STRASS trial, it did reduce rates of local recurrence. However, the risk of toxicity was substantial and the time to surgery was prolonged. A combination of hypofractionation and proton therapy may reduce delays from the initiation of radiation to surgery and limit the dose to surrounding organs at risk (OARs). We conducted a dosimetric comparison of the pre-operative ultra-hypofractionated intensity-modulated photon (IMRT) and proton radiotherapy (IMPT). Methods: Pre-operative IMRT and IMPT plans were generated on 10 RPS patients. The prescription was 25 Gy radiobiological equivalents (GyEs) (radiobiological effective dose of 1.1) to the clinical target volume and 30 GyEs to the margin at risk, all in five fractions. Comparisons were made using student T-tests. Results: The following endpoints were significantly lower with IMPT than with IMRT: mean doses to liver, bone, and all genitourinary and gastrointestinal OARs; bowel, kidney, and bone V5–V20; stomach V15; liver V5; maximum doses to stomach, spinal canal, and body; and whole-body integral dose. Conclusions: IMPT maintained target coverage while significantly reducing the dose to adjacent OARs and integral dose compared to IMRT. A prospective trial treating RPS with pre-operative ultra-hypofractionated IMPT at our institution is currently being pursued.
SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-κB- and PRMT5-Mediated PGC1α Reduction
Authors: Tamiya Hironari , Urushihara Naoko , Shizuma Kazuko , Ogawa Hisataka , Nakai Sho , Wakamatsu Toru , Takenaka Satoshi , Kakunaga Shigeki
Published: July 2023

Sarcoma is a rare type of cancer for which new therapeutic agents are required. Ferroptosis is a nonapoptotic cell death triggered by iron-mediated lipid peroxidation. We found that TFRC, an iron uptake protein, was expressed at higher levels in sarcoma cell lines than in noncancer and carcinoma cell lines. Glutathione peroxidase 4 (GPX4) protects cells against ferroptosis, and its inhibition using RAS-selective lethal 3 (RSL3) had an antitumor effect that was more pronounced in sarcoma cell lines, particularly synovial sarcoma cells, compared to non-sarcoma cells. Because NF-κB can provoke ferroptosis, we examined the role of SHARPIN, an activator of NF-κB, in sarcoma. We found that SHARPIN expression was significantly associated with reduced survival in cohorts of patients with cancer, including sarcoma. In addition, SHARPIN promoted the sensitivity of sarcoma cells to ferroptosis. Further analyses revealed that the PGC1α/NRF2/SLC7A11 axis and BNIP3L/NIX-mediated mitophagy are regulated through NF-κB and PRMT5 downstream of SHARPIN. Our findings suggest that ferroptosis could have a therapeutic effect in sarcoma, particularly in subpopulations with high TFRC and SHARPIN expression.
Simplified Method for Determining Thermal Stresses during the Construction of Massive Monolithic Foundation Slabs
Authors: Chepurnenko Anton , Turina Vasilina
Published: July 2023

For massive monolithic foundation slabs, the problem of early cracking due to the intense heat release of concrete during the hardening process is relevant. The purpose of this article is to develop a simplified method for determining thermal stresses during the construction of massive monolithic foundation slabs. The proposed technique is based on the hypothesis of parabolic temperature distribution over the thickness of the structure at each moment of time. In addition to the parabolic distribution, the half-wave cosine distribution is also used. A hypothesis is also introduced about the same conditions of heat exchange with the environment on the lower and upper surfaces of the foundation. As a result, formulas are obtained that establish a direct relationship between thermal stresses and the temperature difference between the center and the surface. The solution to the test problem for the foundation slab is presented and compared with an alternative technique that does not use the hypothesis about the character of the temperature distribution over the thickness. Also, the inverse problem of determining the allowable temperature drop between the center and the surface of the structure is solved, at which the stresses on the upper surface at each moment of time will not exceed the tensile strength of concrete.
Effect of Gelling Agent Type on the Physical Properties of Nanoemulsion-Based Gels
Authors: Riquelme Natalia , Savignones Constanza , López Ayelén , Zúñiga Rommy N. , Arancibia Carla
Published: July 2023

Senior populations may experience nutritional deficiencies due to physiological changes that occur during aging, such as swallowing disorders, where easy-to-swallow foods are required to increase comfort during food consumption. In this context, the design of nanoemulsion-based gels (NBGs) can be an alternative for satisfying the textural requirements of seniors. This article aimed to develop NBGs with different gelling agents, evaluating their physical properties. NBGs were prepared with a base nanoemulsion (d = 188 nm) and carrageenan (CA) or agar (AG) at two concentrations (0.5–1.5% w/w). The color, rheology, texture, water-holding capacity (WHC) and FT-IR spectra were determined. The results showed that the CA-based gels were more yellow than the AG ones, with the highest hydrocolloid concentration. All gels showed a non-Newtonian flow behavior, where the gels’ consistency and shear-thinning behavior increased with the hydrocolloid concentration. Furthermore, elastic behavior predominated over viscous behavior in all the gels, being more pronounced in those with AG. Similarly, all the gels presented low values of textural parameters, indicating an adequate texture for seniors. The FT-IR spectra revealed non-covalent interactions between nanoemulsions and hydrocolloids, independent of their type and concentration. Finally, the CA-based gels presented a higher WHC than the AG ones. Therefore, NBG physical properties can be modulated according to gelling agent type in order to design foods adapted for seniors.
The Impact on Cow Performance and Feed Efficiency When Individual Cow Milk Composition and Energy Intake Are Accounted for When Allocating Concentrates
Authors: Craig Aimee-Louise , Gordon Alan W. , Ferris Conrad P.
Published: July 2023

The objective of this three-treatment, 12-week study (involving 69 dairy cows) was to test three methods of concentrate allocation on milk production efficiency. All treatments were offered a basal mixed ration of grass silage and concentrates, with additional concentrates offered to individual cows based on either milk yield alone (Control), milk energy output (Precision 1) or energy intake and milk energy output (Precision 2). Concentrate requirements were calculated and adjusted weekly. Control cows had lower concentrate dry matter intake (DMI; p = 0.040) and milk protein content (p = 0.003) but yield of milk and energy-corrected milk (ECM), energy balance, bodyweight and condition score were unaffected by treatment. Efficiency measures such as ECM/DMI and ECM/metabolizable energy intake were also unaffected by treatment. Less concentrates were used per kg ECM yield in the Control compared to the Precision treatments (p < 0.001). In conclusion, accounting for individual cow milk composition or milk composition combined with individual cow energy intake did not improve production efficiency compared to an approach based on individual cow milk yield only.
Feature Papers in Ecology 2022 and the Remarkable Journal’s Advancements
Authors: Arévalo José Ramón
Published: July 2023

The journal Ecologies experienced continuous growth throughout the year 2022 [...]