bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspects of Fe-Incorporation into CaTiO3-SrTiO3 Perovskites and Their Catalytic Application for Ammonia SCO/SCR
Authors: Gwóźdź Paulina , Łącz Agnieszka , Górecka Sylwia , Pacultová Kateřina , Górecki Kamil , Obalová Lucie , Drożdż Ewa
Published: November 2024

Perovskite materials in the CaTiO3-SrTiO3 system doped with different amounts of iron (1, 2 and 5 mol.%) and various Ca/Sr ratios were prepared by the modified citrate method. Additionally, the materials with 0.05 deficiency in strontium/calcium sublattice and 5 mol.% of Fe were also synthesised. The materials were subjected to structural (XRD, XANES) and microstructural (SEM) characterisation, as well as the analysis of susceptibility to reduction/oxidation processes. The structural analysis indicates a lack of iron-containing phases; thus, an incorporation of Fe into the perovskite structure was postulated. Additionally, the oxidation state of iron in the perovskite structure changes with the dopant amount. The temperature-programmed reduction measurements showed partial reversibility of the reduction processes. For the materials with the highest iron amount, the catalytic tests in NH3-SCO and NH3-SCR reactions were carried out. The materials showed high catalytic activity and high selectivity to N2 in the NH3-SCR process; however, they were inactive in NH3-SCO.
Unconventional C—Hlg···H–C (Hlg = Cl, Br, and I) Interactions Involving Organic Halides: A Theoretical Study
Authors: Burguera Sergi , Bauzá Antonio
Published: November 2024

In this study, unconventional C—Hlg···H–C (Hlg = Cl, Br, and I) interactions involving sp, sp2, and sp3 organic halides were investigated at the RI-MP2/aug-cc-pVQZ level of theory. Energy Decomposition Analyses (EDA) and Natural Bonding Orbital (NBO) studies showed that these intermolecular contacts are mainly supported by orbital and dispersion contributions, which counteracted the unfavorable/slightly favorable electrostatics due to the halogen–hydrogen σ-hole facing. In addition, the Bader’s Quantum Theory of Atoms in Molecules (QTAIM) and the Noncovalent Interaction plot (NCIplot) visual index methodologies were used to further characterize the interactions discussed herein. We expect that the results reported herein will be useful in the fields of supramolecular chemistry, crystal engineering, and rational drug design, where the fine tuning of noncovalent interactions is crucial to achieve molecular recognition or a specific solid-state architecture.
Effect of Selenium Supplementation on Biotin and Selenobiotin Concentrations in Meyerozyma guilliermondii and Trichosporon cutaneum Cells
Authors: Patelski Andrea Maria , Dziekońska-Kubczak Urszula , Nowak Agnieszka , Ditrych Maciej , Balcerek Maria , Pielech-Przybylska Katarzyna , Dziugan Piotr
Published: November 2024

Numerous studies have demonstrated the efficacy of selenium compounds in preventing and treating lifestyle-related diseases such as cancer and cardiovascular disorders. The formulation of selenium-enriched supplements for humans and animals, particularly those containing selenium yeast, is highly advantageous. These products are rich in organic selenium derivatives, showing significantly higher bioavailability than inorganic forms of selenium. A particularly promising selenium analogue of sulphur-containing compounds is selenobiotin. The literature indicates that Phycomyces blakesleeanus and Escherichia coli strains can synthesise this compound. This research aimed to evaluate the effect of selenium supplementation on the biosynthesis of biotin and selenobiotin in Trichosporon cutaneum and Meyerozyma guilliermondii. The results have the potential to advance biotechnological approaches for the production of selenobiotin for various applications. A method based on affinity chromatography was used to quantify selenobiotin. The results confirmed that both yeast strains could synthesise selenobiotin in addition to biotin. In M. guilliermondii cells, selenobiotin accounted for up to 17.3% of the total biotin vitamer fraction. In comparison, in T. cutaneum cells, it accounted for up to 28.4% of the sum of biotin and its analogues. The highest levels of selenobiotin were observed in cells cultured with selenomethionine.
In Vitro Antibacterial Activity, Molecular Docking, and ADMET Analysis of Phytochemicals from Roots of Dovyalis abyssinica
Authors: Belitibo Dereilo Bekere , Meressa Asfaw , Abebe Abiy , Negassa Temesgen , Endale Milkyas , Assamo Frehiwot Teka , Wolde-Mariam Messay , Ayana Temesgen Abdisa , Frese Marcel , Sewald Norbert , Abdissa Negera
Published: November 2024

Dovyalis abyssinica is widely used in Ethiopia for treating various human ailments, yet its pharmacological properties and chemical composition remain largely unexplored. The chromatographic separation of D. abyssinica roots extract afforded five compounds, namely tremulacin (1), cochinchiside A (2), 5-methoxydurmillone (3), catechin-7-O-α-L-rhamnopyranoside (4), and stigmasterol (5), confirmed via IR, NMR, and MS spectral data. This is the first report of these compounds from this plant, except for compounds 1 and 5. The extracts and isolated compounds were tested for antibacterial activity against S. aureus, S. epidermidis, E. faecalis, E. coli, K. pneumoniae, and P. aeruginosa strains. Methanol roots extract exhibited significant antibacterial activity (MIC 0.195 mg/mL) against E. coli and P. aeruginosa. Compounds 1 and 3 showed remarkable antibacterial activity, with compound 1 (MIC 0.625 mg/mL) exhibiting antibacterial activity against S. aureus and S. epidermidis, whereas compound 3 (MIC 0.625 mg/mL) exhibited antibacterial activity against S. epidermidis and K. pneumoniae. Molecular docking analysis revealed better binding energies for compound 1 (−8.0, −9.7, and −8.0 kJ/mol) and compound 3 (−9.0, −8.7, and −8.4 kJ/mol), compared to ciprofloxacin (−8.3, −7.5, and −6.7 kJ/mol), in regard to S. aureus pyruvate kinase, S. epidermidis FtsZ, and K. pneumoniae Topoisomerase IV, respectively. ADME analysis also revealed good antibacterial candidacy of these compounds, provided that in vivo analysis is conducted for further confirmation of the results.
Simulation of Solvatochromic Phenomena in Xanthione Using Explicit Solvent Methods
Authors: Manian Anjay , Chen Zifei , Hudson Rohan J. , Russo Salvy P.
Published: November 2024

Xanthione is a sulfated polycyclic aromatic hydrocarbon which exhibits unique anti-Kasha properties and substantial sensitivity to its medium. Due to this sensitivity however, this makes xanthione-based systems very difficult to simulate. Further, xanthione’s is understood to be come more photostable in the presence of a highly polar medium, however whether these photophysical properties could be taken advantage of for certain applications remains to be seen. In clarifying long-held beliefs of specific solvent effects, we apply a rigorous theoretical solvent analysis in both implicit and explicit solvent mediums to elucidate a more complete description of solvent polarity sensitivity in xanthione using both quantum chemical and molecular dynamics techniques. Not only was it found that explicit solvation methods are vital in an accurate description of the system, only a handful of explicit solvent molecules in the simulation are required to yield an appropriate electronic description. This short work is vital to devising future applications for xanthione-based and other quantum technologies, and is an important foundation stone on this journey.
Pimozide and Adipic Acid: A New Multicomponent Crystalline Entity for Improved Pharmaceutical Behavior
Authors: Buscarini Alessandra , Zaworotko Michael J. , Matos Catiúcia R. M. O. , Grepioni Fabrizia , Contini Laura , Capsoni Doretta , Friuli Valeria , Maggi Lauretta , Bruni Giovanna
Published: November 2024

Pimozide is a first-generation antipsychotic used in the treatment of schizophrenia, Gilles de la Tourette syndrome, and other chronic psychoses. Its in vivo efficacy is limited by poor solubility and consequent poor bioavailability. Therefore, adipic acid was used as a coformer for the preparation of a binary product with improved pharmaceutical properties. The thermal behavior of the liquid-assisted grinding products of compositions included in the range 0.1 < XPMZ < 0.9 has been interpreted using a thermo-dynamic model according to which the two components originate a new crystalline entity in molar ratio pimozide:adipic acid 0.66:0.33, which forms an eutectic system with adipic acid. The model was confirmed using the quantitative analysis of the melting peaks and using the X-ray diffraction measurements from powders and single crystals. In particular, the latter have demonstrated that the new entity resulting from the pimozide:adipic acid 0.66:0.33 composition is actually salt [PMZH]2[adipate]. The crystalline product was characterized, from a pharmaceutical perspective, in terms of solubility and wettability (contact angle). Then, a tablet formulation was developed, and its dissolution behavior was compared to a commercial product considered as a reference. The new entity showed improved pharmaceutical properties in terms of solubility and wettability compared to the pure drug in both deionized water and bio-relevant fluids simulating oral administration in fed and fasted conditions. The tablets containing the new crystalline form can make this virtually insoluble drug available for absorption within minutes regardless of the variability in gastric conditions.
The Valorisation of Biochar Produced from Black Liquor Pyrolysis for the Development of CO2 Adsorbents
Authors: Zaharioiu Anca Maria , Niculescu Violeta-Carolina , Sandru Claudia , Spiridon Stefan Ionut , Soare Amalia , Oancea Simona , Marin Florian
Published: November 2024

The paper manufacturing process produces liquid and gaseous alternative fuels, as well as solid wastes. These can be subsequently treated through chemical processing, oxidation, and thermal activation, resulting in adsorbent materials with CO2 adsorption capacities. The valorisation of black liquor waste resulting from paper manufacturing was achieved through a catalytic pyrolysis process using two catalysts previously prepared in house (Cu-Zn-MCM-41 and Ni-SBA-16). The HCl-treated adsorbent material, resulting from Ni-SBA-16-catalysed pyrolysis, was selected for use in CO2 adsorption tests as it had the highest specific surface area (224.06 m2/g) and pore volume (0.28 cm3/g). The adsorption experimental setup was linked to a gas chromatograph in order to evaluate CO2 adsorption efficiency using a binary gas mixture consisting of 81% CO2 and 19% N2. With a CO2 adsorption capacity of 1.61 mmol/g, a separation efficiency of 99.78%, and a CO2 recovery yield of 90.02%, it can be concluded that the developed adsorbent material resulting from Ni-SBA16-catalysed pyrolysis and HCl treatment represents a viable solution for black liquor pyrolytic solid waste removal and reduction in greenhouse gases.
Quantum Otto Heat Engine Using Polar Molecules in Pendular States
Authors: Li Xiang , Sun Zhaoxi , Fang Yu-Yan , Huang Xiao-Li , Huang Xinning , Li Jin-Fang , Zhang Zuo-Yuan , Liu Jin-Ming
Published: November 2024

Quantum heat engines (QHEs) are established by applying the principles of quantum thermodynamics to small−scale systems, which leverage quantum effects to gain certain advantages. In this study, we investigate the quantum Otto cycle by employing the dipole−dipole coupled polar molecules as the working substance of QHE. Here, the molecules are considered to be trapped within an optical lattice and located in an external electric field. We analyze the work output and the efficiency of the quantum Otto heat engine (QOHE) as a function of various physical parameters, including electric field strength, dipole−dipole interaction and temperatures of heat baths. It is found that by adjusting these physical parameters the performance of the QOHE can be optimized effectively. Moreover, we also examine the influences of the entanglement and relative entropy of coherence for the polar molecules in thermal equilibrium states on the QOHE. Our results demonstrate the potential of polar molecules in achieving QHEs.
Tumor-Targeted Cell-Penetrating Peptides Reveal That Monomethyl Auristatin E Temporally Modulates the Tumor Immune Microenvironment
Authors: Mortaja Mahsa , Cheng Marcus M. , Ali Alina , Lesperance Jacqueline , Hingorani Dina V. , Allevato Mike M. , Dhawan Kanika , Camargo Maria F. , McKay Rana R. , Adams Stephen R. , Gutkind J. Silvio , Advani Sunil J.
Published: November 2024

Chemotherapies remain standard therapy for cancers but have limited efficacy and cause significant side effects, highlighting the need for targeted approaches. In the progression of cancer, tumors increase matrix metalloproteinase (MMP) activity. Leveraging and therapeutically redirecting tumor MMPs through activatable cell-penetrating peptide (ACPP) technology offers new approaches for tumor-selective drug delivery and for studying how drug payloads engage the tumor immune microenvironment. ACPPs are biosensing peptides consisting of a drug-conjugated polycationic cell-penetrating peptide masked by an autoinhibitory polyanionic peptide through an interlinking peptide linker. Since tumors overexpress MMPs, ACPP tumor-targeting is achieved using an MMP cleavable linker. Monomethyl auristatin E (MMAE) is a potent anti-tubulin and common drug payload in antibody drug conjugates; however there are limited pre-clinical studies on how this clinically effective drug modulates the interplay of cancer cells and the immune system. Here, we report the versatility of ACPP conjugates in syngeneic murine cancer models and interrogate how MMAE temporally alters the tumor immune microenvironment. We show that cRGD-ACPP-MMAE preferentially delivered MMAE to tumors in murine models. Targeted cRGD-ACPP-MMAE demonstrated anti-tumor kill activity that activated the innate and adaptive arms of the immune system. Understanding how targeted MMAE engages tumors can optimize MMAE tumor kill activity and inform rational combinations with other cancer therapeutics.
Nanomaterials and Textiles
Authors: Mahltig Boris , Ehrmann Andrea
Published: November 2024

The terms “nano” and “nanomaterials” have come to be buzz words describing tremendous advances in research and development during the last decades [...]
Synthesis and Application of Optical Nanomaterials
Authors: Jun Bong-Hyun
Published: November 2024

The field of optical nanomaterials stands at the forefront of technological innovation, driving advancements across a spectrum of scientific disciplines and industries [...]
Daily Amount of Purine in Commonly Recommended Well-Balanced Diets in Japan and Overseas
Authors: Kaneko Kiyoko , Tsuruga Keito , Takayanagi Fukue , Fukuuchi Tomoko , Yamaoka Noriko , Seki Reiko , Fujimori Shin
Published: November 2024

Background/Objectives: A low purine diet has been recommended for patients with gout and hyperuricemia, but there are concerns about excessive carbohydrates and a lack of protein. A well-balanced diet in accordance with general dietary guidelines is widely recommended. The Mediterranean diet and the DASH (Dietary Approaches to Stop Hypertension) diet are also recommended for gout and hyperuricemia patients. However, there is little information on the purine levels in these diets. The aim of this study is to determine the daily amount of purine in well-balanced diets that follows the dietary guidelines, including the Mediterranean diet and the DASH diet. Methods: We measured the purine content in various foodstuffs. Using these values, we calculated the amount of purine in generally recommended well-balanced diets—the Japanese diet, American My Plate Plan, the Mediterranean diet, and the DASH diet. To calculate the amount of purine, recipes tailored to the characteristics of each diet were created. Results: The amount of purine in the Japanese diet, in the American My Plate Plan, in the Mediterranean diet, and in the DASH diet were 308.5–366.1, 308.7–335.0, 346.6–394.1, 325.9–493.9 mg/day, respectively. These values were close to the 400 mg/day recommended in the Japanese Guideline for the management of Hyperuricemia and Gout. Conclusions: A well-balanced diet following the recommendation in the dietary guidelines is considered to provide adequate purines. Because a high purine diet can lead to the recurrence of gout, advising to eat a balanced diet is useful on lifestyle guidance for the patients with gout and hyperuricemia.
Dietary Phytic Acid, Dephytinization, and Phytase Supplementation Alter Trace Element Bioavailability—A Narrative Review of Human Interventions
Authors: Chondrou Thiresia , Adamidi Nikoleta , Lygouras Dimosthenis , Hirota Simon A. , Androutsos Odysseas , Svolos Vaios
Published: November 2024

Background: Phytic acid is abundant in plant-based diets and acts as a micronutrient inhibitor for humans and non-ruminant animals. Phytases are enzymes that break down phytic acid, releasing micronutrients and enhancing their bioavailability, particularly iron and zinc. Deficiencies in iron and zinc are significant public health problems, especially among populations with disease-associated malnutrition or those in developing countries consuming phytic acid-rich diets. This narrative review aimed to summarize findings from human intervention studies on the interactions between phytic acid, phytase, and micronutrient bioavailability. Methods: An extensive PubMed search (1 January 1990 to 8 February 2024) was conducted using MeSH terms (phytic acid, phytase, IP6, “inositol hexaphosphate,” micronutrient, magnesium, calcium, iron, zinc). Eligible studies included human intervention trials investigating the bioavailability of micronutrients following (a) phytase supplementation, (b) consumption of phytic acid-rich foods, or (c) consumption of dephytinized foods. In vitro, animal, cross-sectional, and non-English studies were excluded. Results: 3055 articles were identified. After the title and full-text review, 40 articles were eligible. Another 2 were identified after cross-checking reference lists from included papers, resulting in 42 included articles. Most studies exploring the efficacy of exogenous phytase (9 of 11, 82%) or the efficacy of food dephytinization (11 of 14, 79%) demonstrated augmented iron and zinc bioavailability. Most phytic acid-rich food-feeding studies (13 of 17, 77%) showed compromised iron and zinc bioavailability. Conclusions: Strong evidence supports decreased iron and zinc bioavailability in phytic acid-rich diets and significant improvements with phytase interventions. Studies of longer periods and within larger populations are needed.
The Impact of Complementary Feeding on Fecal Microbiota in Exclusively Breast-Fed Infants with Cystic Fibrosis (A Descriptive Study)
Authors: Asensio-Grau Andrea , Garriga María , Vicente Saioa , Andrés Ana , Ribes-Koninckx Carmen , Calvo-Lerma Joaquim
Published: November 2024

Background/Objectives: Early life gut microbiota plays a pivotal role in shaping immunity, metabolism, and overall health outcomes. This is relevant in healthy infants but may be even more crucial in infants with chronic devastating diseases, such as cystic fibrosis (CF). While the introduction of solid foods in healthy infants modifies the composition of colonic microbiota, less knowledge is available on those with CF. The aim of this descriptive observational study was to assess the composition of fecal microbiota in six exclusively breast-fed infants with CF, and then explore the changes induced upon the introduction of different foods. Methods: two types of fecal samples were collected from each subject: one during the exclusive-breastfeeding period, and the other after incorporating each new food in the ad libitum diet. The microbiota composition was analyzed by 16S rRNA amplicon sequencing. Results: Wide heterogenicity in the composition at the phylum level (variable proportions of Actinobacteriota, Proteobacteria, and Firmicutes, and the absence of Bacteroidota in all subjects) was found, and different enterotypes were characterized in each subject by the main presence of one genus: Bifidobacterium in Subject 1 (relative abundance of 54.4%), Klebsiella in Subject 3 (49.1%), Veillonella in Subjects 4 and 5 (32.7% and 36.9%, respectively), and Clostridium in Subject 6 (48.9%). The transition to complementary feeding induced variable changes in microbiota composition, suggesting a subject-specific response and highlighting the importance of inter-individual variation. Conclusions: Further studies are required to identify which foods contribute to shaping colonic microbiota in the most favorable way for patients with CF using a personalized approach.
Vitamin D Supplementation as a Therapeutic Strategy in Autoimmune Diabetes: Insights and Implications for LADA Management
Authors: Mourelatou Niki G. , Kounatidis Dimitris , Jude Edward B. , Rebelos Eleni
Published: November 2024

Latent autoimmune diabetes of adults (LADA) is the most prevalent form of autoimmune diabetes (AI-D) in adulthood; however, its accurate diagnosis and optimal treatment remain challenging. Vitamin D deficiency (VDD) is commonly observed in LADA patients, while increased vitamin D exposure through supplementation and dietary intake is associated with a reduced incidence of LADA. Although limited, case reports, case-control studies, and randomized clinical trials have examined the effects of vitamin D supplementation—alone or combined with dipeptidyl peptidase-4 inhibitors (DPP4-is)—on glucose regulation, residual β-cell function, and glutamic acid decarboxylase antibody (GADA65) levels. Findings, while preliminary, indicate that vitamin D supplementation may enhance glycemic control, preserve β-cell function, and reduce autoimmune activity. Given its accessibility, affordability, and relative safety, vitamin D supplementation presents an attractive adjunct treatment option for LADA patients. This narrative review discusses current evidence on the potential therapeutic benefits of vitamin D supplementation in patients with AI-D, including LADA, who are also vitamin D deficient. Beginning with an exploration of the epidemiological patterns, clinical presentation, and diagnostic framework essential for understanding and identifying LADA, this review then examines the proposed mechanisms through which vitamin D may influence autoimmune modulation of pancreatic β-cells, integrating recent data pertinent to LADA pathology. By distilling and consolidating existing research, we aim to provide a platform for advancing targeted investigations within this distinct patient population.
Protective Effects of Spirulina Against Lipid Micelles and Lipopolysaccharide-Induced Intestinal Epithelium Disruption in Caco-2 Cells: In Silico Molecular Docking Analysis of Phycocyanobilin
Authors: Arrari Fatma , Ortiz-Flores Rodolfo-Matias , Lhamyani Said , Garcia-Fuentes Eduardo , Jabri Mohamed-Amine , Sebai Hichem , Bermudez-Silva Francisco-Javier
Published: November 2024

Damage to intestinal epithelial cells is present in obesity and other diseases because of inflammatory and oxidative processes. This damage compromises the gastrointestinal barrier, killing enterocytes, altering intestinal permeability, and eliciting abnormal immune responses that promote chronic inflammation. Recent evidence shows that spirulina is a potent natural agent with antioxidant and anti-inflammatory properties. Objectives: This study was conducted to evaluate the effect of spirulina aqueous extract (SPAE) on the alterations of the intestinal epithelium induced by lipid micelles (LMs) and/or inflammation induced by lipopolysaccharides (LPSs) in the Caco-2 cell line. Methods: In the current research, we assessed the protective actions of SPAE against cytotoxicity, oxidative stress, inflammation, and epithelial barrier perturbation by using an in vitro model, the intestinal Caco-2 cells, treated with LPSs and/or LMs. We also performed an in silico molecular docking analysis with spirulina’s bioactive compound, phycocyanobilin. Results: Our results showed that SPAE has no cytotoxic effect on Caco-2 cells. On the contrary, it improved cell viability and exhibited anti-inflammatory and antioxidant actions. SPAE also protected against endoplasmic reticulum stress and tight junction proteins, thus improving the epithelial barrier. The in silico study revealed a strong binding affinity of the phycocyanobilin compound with human SOD and NADPH oxidase and a good binding affinity towards COX-2 and iNOS. Conclusions: Taken together, these findings demonstrate the beneficial actions of SPAE on Caco-2 cells, suggesting it may be useful in preserving the epithelial intestinal barrier in human conditions involving oxidative stress and inflammation such as obesity.
Resveratrol Butyrate Esters Reduce Hypertension in a Juvenile Rat Model of Chronic Kidney Disease Exacerbated by Microplastics
Authors: Huang Yi-Ning , Hsu Chien-Ning , Hou Chih-Yao , Chen Shin-Yu , Tain You-Lin
Published: November 2024

Background: Resveratrol is recognized as a promising nutraceutical with antihypertensive and prebiotic properties; however, its bioavailability in vivo is limited. To enhance its bioactivity, we developed resveratrol butyrate esters (RBEs). This study investigates whether RBEs can mitigate hypertension induced by chronic kidney disease (CKD) and exacerbated by microplastics (MPs) exposure in juvenile rats. Methods: Three-week-old male Sprague Dawley rats were fed either regular chow or 0.5% adenine chow for three weeks. The adenine-fed CKD rats (N = 8 per group) received either 5 μM MPs (10 mg/L) or MPs combined with RBE (25 mg/L) in their drinking water from weeks 3 to 9. Results: Our results indicate that MP exposure worsened CKD-induced hypertension, while RBE treatment resulted in a reduction in systolic BP by 15 mmHg (155 ± 2 mmHg vs. 140 ± 1 mmHg, p < 0.05). The combined exposure to adenine and MPs was associated with nitric oxide (NO) deficiency, which RBE treatment alleviated. Additionally, our findings revealed that RBE modulated both the classical and nonclassical renin–angiotensin system (RAS), contributing to its protective effects. We also observed changes in gut microbiota composition, increased butyric acid levels, and elevated renal GPR41 expression associated with RBE treatment. Conclusions: In conclusion, in this juvenile rat model of combined CKD and MP exposure, RBE demonstrates antihypertensive effects by modulating NO levels, the RAS, gut microbiota, and their metabolites.
Microencapsulation of Anthocyanins from Zea mays and Solanum tuberosum: Impacts on Antioxidant, Antimicrobial, and Cytotoxic Activities
Authors: Barba-Ostria Carlos , Carrero Yenddy , Guamán-Bautista Jéssica , López Orestes , Aranda Christian , Debut Alexis , Guamán Linda P.
Published: November 2024

Objectives: This study investigates the biological activities of microencapsulated anthocyanins extracted from two Andean ancestral edible plants, Solanum tuberosum, and Zea mays, with a focus on their potential applications in functional foods and therapeutics. The primary objective was to evaluate their antioxidant, antimicrobial, and cytotoxic properties alongside structural and functional analyses of the microencapsulation process. Methods: Anthocyanins were extracted and microencapsulated using maltodextrin as a carrier. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were employed to analyze the stability and structure of the microencapsulated particles. The antioxidant, antimicrobial, and cytotoxic activities of the microencapsulated were assessed through established assays. Results:S. tuberosum exhibited superior antioxidant capacity and potent anticancer activity against HepG2 and THJ29T cell lines, while Z. mays demonstrated significant antimicrobial efficacy against multidrug-resistant bacterial strains and biofilm-forming pathogens. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) confirmed the stabilization of anthocyanins within a maltodextrin matrix, enhancing their bioavailability and application potential. Conclusions: These results highlight the versatility of microencapsulated anthocyanins as bioactive agents for industrial and therapeutic applications. Future studies should explore in vivo validation and synergistic formulations to optimize their efficacy and broaden their use in nutraceutical and pharmaceutical fields.
Influence of Alkaline Reduced Water Supplementation on Glucose and Lipid Metabolism in Non-Diabetic and Diabetic Rats
Authors: Huang Chung-Hsiung , Chao Ying-Chiun , Chiang Meng-Tsan
Published: November 2024

Background: With the global increase in metabolic disorders, identifying effective dietary strategies is crucial for enhancing health outcomes. While various health advantages of alkaline reduced water (ARW) have been documented, its specific impacts on glucose and lipid metabolism in both healthy and diabetic conditions are still not well understood. Methods: This study investigates how ARW affects carbohydrate and lipid metabolism in male Wistar rats, which were induced to develop glucose metabolism disorders through subcutaneous injections of nicotinamide and streptozotocin (STZ). The rats were allocated into four groups: one group received distilled water, another ARW, with similar arrangements for both non-diabetic and diabetic rats. Throughout the six-week experiment, the rats had unrestricted access to food and water. At the end of the study, blood and tissue samples were collected post-euthanasia for further analysis. Results: Non-diabetic rats consuming ARW experienced significant decreases in plasma glucose, triglycerides, cholesterol, insulin, leptin, and TBARS levels, along with reduced activities of hepatic hexokinase and intestinal sucrase. Meanwhile, there were increases in hepatic antioxidant enzyme activities, such as glutathione peroxidase and glucose-6-phosphate dehydrogenase, although glutathione levels decreased. In diabetic rats, ARW supplementation notably reduced plasma glucose and the glucose area under the curve, lowered hepatic glucose-6-phosphatase and intestinal disaccharidase activities, and raised hepatic GSH levels. Conclusions: These findings suggest that ARW supplementation significantly enhances glucose and lipid metabolism and boosts antioxidant activity in both non-diabetic and diabetic rats, indicating its potential as a therapeutic aid for managing metabolic disorders.
Limosilactobacillus fermentum MG5368 and Lactiplantibacillus plantarum MG989 Regulates Skin Health in UVB-Induced HaCaT Cells and Hairless Mice Model
Authors: Park Jeong-Yong , Lee Ji Yeon , Hong Seonghwa , Heo Huijin , Lee Hana , Kim Yong Gyeong , Kim Byoung-Kook , Choi Soo-Im , Lee Junsoo
Published: November 2024

Background: Photoaging, induced by chronic ultraviolet B (UVB) exposure, results in the degradation of extracellular matrix (ECM) components, leading to skin roughness, wrinkle formation, and reduced elasticity. Recent studies have explored probiotics as potential inhibitors of extrinsic aging, primarily through mechanisms that protect the skin barrier and reduce collagen breakdown. Methods: This study investigates the anti-photoaging effects of Limosilactobacillus fermentum MG5368 (L. fermentum MG5368) and Lactiplantibacillus plantarum MG989 (L. plantarum MG989) in UVB-exposed keratinocytes and an SKH-1 hairless mice model. Results: Both strains demonstrated significant efficacy in preserving collagen through the inhibition of activating protein-1 (AP-1) and reducing the expression of matrix metalloproteinase (MMP)-1 and MMP-3. Additionally, both strains restored COL1A1 protein expressions, thereby enhancing collagen synthesis and ECM stability. Enhanced skin elasticity was observed, attributed to restored levels of hyaluronic acid and hyaluronan synthase 2 (HAS2) protein expressions. Conclusions: These findings suggest that L. fermentum MG5368 and L. plantarum MG989 may serve as promising probiotic-based agents for anti-photoaging applications.
Vitamin D and Pancreatic Ductal Adenocarcinoma—A Review of a Complicated Relationship
Authors: Grosu Iustina , Constantinescu Alexandru , Balta Mihaela Daniela , Băjenaru Ovidiu , Nuța Cătălina , Pavel Christopher , Sandru Vasile , Munteanu Mihai , Andronic Octavian
Published: November 2024

Introduction: From the observation of a negative relationship between UV-B exposure and cancer rates, we hypothesized that vitamin D (VD) may play a protective role in oncogenesis. Moreover, repurposing a well-known and relatively safe drug for conditions with dismal prospects, such as pancreatic ductal adenocarcinoma (PDAC), is a tempting idea. Thus, we aimed to summarize the current knowledge regarding the role of VD in the prevention and treatment of PDAC. Methods: We conducted a systematic review of VD and PDAC using Medline-indexed studies accessed through PubMed as the primary data source. This study aimed to identify articles focusing on the role of VD as a risk and prognostic factor for PDAC, mechanistic studies evaluating the effects of VD or vitamin D analogs (VDAs) in PDAC models, and clinical trials on VDAs in PDAC. After the screening, 97 studies were included in the final manuscript. Conclusion: Even though the results from epidemiologic studies were contradictory, basic research has demonstrated that VD can act on PDAC cells either directly, inhibiting proliferation, apoptosis, EMT, migration, invasion, and stemness, or indirectly, through stromal remodeling. A better understanding of the consequences of VD-induced tumor–stroma cross-talk alterations is needed to determine whether VD/VDAs can be used to our own advantage in the treatment of PDAC.
Sirtuin Proteins and Memory: A Promising Target in Alzheimer’s Disease Therapy?
Authors: Fernandez Francesca , Griffiths Lyn R. , Sutherland Heidi G. , Cole Michael H. , Fitton J. Helen , Winberg Pia , Schweitzer Daniel , Hopkins Lloyd N. , Meyer Barbara J.
Published: November 2024

Sirtuins (SIRTs), nicotine adenine dinucleotide (+)-dependent histone deacetylases, have emerged as critical regulators in many signalling pathways involved in a wide range of biological processes. Currently, seven mammalian SIRTs have been characterized and are found across a number of cellular compartments. There has been considerable interest in the role of SIRTs in the brain due to their role in a plethora of metabolic- and age-related diseases, including their involvement in learning and memory function in physiological and pathophysiological conditions. Although cognitive function declines over the course of healthy ageing, neurological disorders including Alzheimer’s disease (AD) can be associated with progressive cognitive impairments. This review aimed to report and integrate recent advances in the understanding of the role of SIRTs in cognitive function and dysfunction in the context of AD. We have also reviewed the use of selective and/or natural SIRT activators as potential therapeutic agents and/or adjuvants for AD.
Association of Social Jetlag with the Dietary Quality Among Korean Workers: Findings from a Nationwide Survey
Authors: Baek Seong-Uk , Yoon Jin-Ha
Published: November 2024

Background/Objectives: Social jetlag, which refers to the misalignment between biological rhythms and social schedule, is linked to an increased risk of metabolic diseases. This cross-sectional study investigated the relationship between social jetlag and workers’ dietary quality. Methods: This secondary data analysis included a sample of workers from the Korea National Health and Nutrition Examination Survey (n = 11,430). Social jetlag was determined by calculating the difference in the sleep midpoint between free days and workdays, based on sleep onset and offset times. The Korean Health Eating Index (KHEI) was calculated based on 24-h recalls, with higher scores indicating superior dietary qualities (range: 0–100). Poor dietary quality was defined as a KHEI below the lowest quartile (<51.6). Linear or logistic regressions were utilized to estimate β or odds ratio (OR), respectively. Results: Among study participants, 12.1% of workers experienced ≥120 min of social jetlag. ≥120 min of social jetlag was associated with a reduced KHEI score compared with 0–59 min (β: −1.23, 95% confidence interval [CI]: −2.16, −0.30). Those with ≥120 min of social jetlag were more likely to have poor dietary quality than those with 0–59 min (OR: 1.27, 95% CI: 1.08, 1.50). Conclusions: Workers experiencing ≥120 min of social jetlag had poorer dietary quality compared with workers with 0–59 min of social jetlag. Therefore, this study suggests that policy efforts are needed to reduce social jetlag among workers in Korea.
Effectiveness of Compassion Training on Stress and Anxiety: A Pre-Experimental Study on Nursing Students
Authors: Gutiérrez-Carmona Andrés , González-Pérez Marta , Ruiz-Fernández María Dolores , Ortega-Galán Angela María , Henríquez Diego
Published: November 2024

Background: Stress and anxiety are common problems among nursing students, affecting their mental health and academic performance. Compassion training has been shown to be effective in reducing these states. Objectives: This study evaluated the effects of a compassion-based course on perceived stress and anxiety (state and trait) among nursing students at a state university in Chile. Methods: A pre-experimental design was implemented with 45 second-year students, who completed evaluations before and after the intervention. The course included 10 sessions involving compassion meditation, mindfulness, self-compassion exercises, and autonomous practice. Results: The results showed significant reductions in and a high effect size regarding perceived stress (Cohen’s d = 0.83) and state anxiety (Cohen’s d = 0.74), while trait anxiety showed a moderate reduction (Cohen’s d = 0.34). Mediation analysis revealed that increases in self-compassion mediated the relationship between autonomous practice time and reductions in stress and trait anxiety. Conclusions: These findings suggest that compassion training is an effective strategy for reducing stress and anxiety in nursing students, improving their emotional regulation and preparation for professional practice. Therefore, it is necessary to include this approach in students’ curricular programs.
Analysis of Nursing Education Curricula in Spain: Integration of Genetic and Genomic Concepts
Authors: Ramírez-Baraldes Estel·la , Ariza-Martin Katerin , García-Gutiérrez Daniel , García-Salido Cristina
Published: November 2024

The integration of genetics and genomics into nursing practice is essential for addressing genetic pathologies and providing personalized patient care. This study aims to analyze the nursing education curricula across Spanish universities to understand how genetic and genomic concepts are incorporated. Using the “Qué estudiar y dónde en la Universidad” (QEDU) database, an official source provided by the Spanish Ministry of Universities, a systematic review of 4720 teaching guides from 118 university centers was conducted. Our findings reveal that 12 centers do not include any genetic or genomic content, while 43% of the remaining centers focus solely on basic genetics. In contrast, 57% cover advanced topics, such as genetic counseling and pharmacogenetics. Teaching methodologies predominantly involve lectures and seminars, with limited practical training. On average, three to six ECTS credits out of the total 240 required for the Nursing Degree are dedicated to genetics and genomics. This study highlights the need for curriculum updates and standardization to ensure comprehensive training in these crucial areas. Improved integration of genetic and genomic education will better prepare nursing professionals to meet the evolving demands of modern healthcare. This study was not registered.
Recognizing a Silent Threat: Canine Ehrlichiosis and Rickettsiosis in Northern Portugal
Authors: Zając Zbigniew , Cabezas-Cruz Alejandro
Published: November 2024

Vector-borne diseases pose significant challenges for both animal and public health worldwide [...]
Silver Nanoparticles from Duddingtonia flagrans: Evaluation of Potential Ovicidal Activity on Toxocara canis Eggs
Authors: Ferraz Carolina Magri , Comério Lara Coslop , Segantine Vinícius Bastos Salles , de Assis João Pedro Barbosa , Costa Silva Laryssa Pinheiro , Bezerra Lara De Nadai Rodrigues , de Araújo Jackson Victor , Vilela Vinícius Longo Ribeiro , Soares Filippe Elias de Freitas , Rossi Gabriel Augusto Marques , Tobias Fernando Luiz , Langoni Helio , Braga Fabio Ribeiro
Published: November 2024

The filtrate of the nematophagous fungus Duddingtonia flagrans produces silver nanoparticles (AgNPs) with nematicidal potential. However, there are currently no reports of its activity against Toxocara canis eggs. The aim of this study was to investigate the potential ovicidal activity of AgNPs–D. flagrans on T. canis eggs. T. canis eggs were obtained from the dissection of the uterus of adult female nematodes. After the biosynthesis of AgNPs, two experimental assays (A and B) were performed. In assay A, the ovicidal activity of AgNPs on eggs was evaluated after 15 and 30 days of interaction. In assay B, the inhibition (development) of the eggs was measured after 30 days of interaction. The results of assay A showed that the AgNPs destroyed an average of 47% of the eggs tested by the end of the experiment, causing significant structural damage. In assay B, an inhibition rate of 88% was observed at the end of 30 days. The results of the ovicidal activity of AgNP–D. flagrans were promising and indicate the potential for future studies on these biomolecules with ovicidal properties.
Natural Deep Eutectic Solvents Combined with Supercritical Carbon Dioxide for the Extraction of Curcuminoids from Turmeric
Authors: Stasiłowicz-Krzemień Anna , Wójcik Julia , Gościniak Anna , Szymański Marcin , Szulc Piotr , Górecki Krzysztof , Cielecka-Piontek Judyta
Published: November 2024

Background: Curcuminoids, the bioactive compounds found in turmeric, exhibit potent antioxidant, anti-inflammatory, and neuroprotective properties. This study aims to enhance the extraction of curcuminoids from turmeric using environmentally friendly solvents supercritical CO2 (scCO2) combined with natural deep eutectic solvents (NADESs) in one process, and to evaluate the resulting biological activity. Methods: A Box–Behnken statistical design was applied to optimize scCO2 extraction conditions—pressure, CO2 volume, and temperature—to maximize curcuminoid yield. Next, the menthol and lactic acid NADESs were selected, and these two solvents were combined into a single turmeric extraction process. The biological activity of the resulting extract was evaluated using antioxidant assays (ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl) and enzyme inhibition assays (acetylcholinesterase, butyrylcholinesterase, and tyrosinase). Toxicity assessments were conducted on the aquatic invertebrates Daphnia pulex, Artemia sp., and Chironomus aprilinus. Results: The most effective extraction was achieved using a menthol–lactic acid NADES as a cosolvent, integrated at a 1:20 ratio of plant material to NADESs while in combination with scCO2. The optimized scCO2–NADES extraction resulted in a high curcuminoid yield (33.35 mg/g), outperforming scCO2 extraction (234.3 μg/g), NADESs ultrasound-assisted extraction (30.50 mg/g), and alcohol-based solvents (22.95–26.42 mg/g). In biological assays, the extract demonstrated significant antioxidant activity and effective inhibition of enzymes (acetylcholinesterase, butyrylcholinesterase, and tyrosinase). Toxicity studies showed a concentration-dependent response, with EC50 for Chironomus aprilinus at the level of 0.098 μL/mL and Daphnia pulex exhibiting high sensitivity to the extract. Conclusions: This study highlights the potential of combining NADESs and scCO2 extraction in one process, demonstrating the effectiveness of scCO2–NADES extraction in maximizing curcuminoid yield and enhancing bioactivity.
Cyclodextrin-Nanosponge-Loaded Cyclo-Oxygenase-2 Inhibitor-Based Topical Gel for Treatment of Psoriatic Arthritis: Formulation Design, Development, and In vitro Evaluations
Authors: Hani Umme , Paramshetti Sharanya , Angolkar Mohit , Alqathanin Wajan Khalid , Alghaseb Reema Saeed , Al Asmari Saja Mohammed , Alsaab Alhanouf A. , Fatima Farhat , Osmani Riyaz Ali M. , Gundawar Ravi
Published: November 2024

Background: Psoriatic arthritis (PsA), a chronic inflammatory disease, mainly affects the joints, with approximately 30% of psoriasis patients eventually developing PsA. Characterized by both innate and adaptive immune responses, PsA poses significant challenges for effective treatment. Recent advances in drug delivery systems have sparked interest in developing novel formulations to improve therapeutic outcomes. The current research focuses on the development and evaluation of a nanosponge-loaded, cyclo-oxygenase-2 (COX-2) inhibitor-based topical gel for the treatment of PsA. Methods: Nanosponges (NSs) were prepared by using beta-cyclodextrin as a polymer and dimethyl carbonate (DMC) as a crosslinker by melting, and gels were prepared by employing carbopol and badam gum as polymers. Results: Solubility studies confirmed that the prepared nanosponges were highly soluble. FT-IR studies confirmed the formation of hydrogen bonds between lumiracoxib and beta-cyclodextrin. SEM confirmed that the prepared formulations were roughly spherical and porous in nature. The average particle size was 190.5 ± 0.02 nm, with a zeta potential of −18.9 mv. XRD studies showed that the crystallinity of lumiracoxib decreased after encapsulation, which helped to increase its solubility. The optimized nanosponges (NS2) were incorporated in an optimized gel (FG10) to formulate a nanosponge-loaded topical gel. The optimized gel formulation exhibited a homogeneous consistency, with a pH of 6.8 and a viscosity of 1.15 PaS, indicating its suitability for topical application and stability. The in vitro diffusion studies for the topical gel showed drug release of 82.32% in 24 h. The optimized formulation demonstrated significant antipsoriatic activity, as confirmed through cytotoxicity studies conducted on HaCaT cells. Conclusions: On the basis of the findings, it can be concluded that the prepared nanosponge-loaded topical gel formulation presents a promising solution for the effective management of PsA, offering enhanced drug solubility, sustained release, and improved therapeutic potential.
Investigating the Antibacterial, Antioxidant, and Anti-Inflammatory Properties of a Lycopene Selenium Nano-Formulation: An In Vitro and In Vivo Study
Authors: Binsuwaidan Reem , El-Masry Thanaa A. , El-Nagar Maysa M. F. , El Zahaby Enas I. , Gaballa Mohamed M. S. , El-Bouseary Maisra M.
Published: November 2024

Background: The potent antioxidant lycopene has attracted a large amount of research attention given its potential health benefits. We aimed to assess the antimicrobial, anti-inflammatory, and antioxidant properties of lycopene (Lyc), selenium nanoparticles (Se-NPs), and lycopene selenium nanoparticles (Lyc-Se-NPs). Methods: FTIR, polydispersity index, and zeta potential evaluations provided a complete characterization of the synthesized Lyc-Se-NPs. The broth dilution method and a crystal violet microtiter plate assay were employed to assess the antibacterial and antibiofilm activity, respectively. The rat wound infection model was performed to study the anti-inflammatory effect. Findings: The Lyc-Se-NPs had a zeta potential range of −16.93 to −31.04 mV and a mean particle size of 126.6 ± 3.12 nm. All peaks’ percentage transmittance decreased, according to the FTIR analysis of the Lyc-Se-NPs, with the exception of one peak at 2924.22 cm−1, which is suggestive of C-H stretching. The mean scavenging concentrations for Lyc-Se-NPs in the DPPH and ABTS radical scavenging experiments were 3.85 ± 0.65 and 4.26 ± 0.7 µg/mL, respectively. For S. aureus, the Lyc-Se-NPs’ MIC values varied from 64 to 1024 µg/mL. CLSM verified that S. aureus treated with sub-MICs of Lyc-Se-NPs showed a significant reduction in biofilm formation. Furthermore, the group treated with 50 mg of Lyc-Se-NPs showed the quickest rate of wound healing. They demonstrated a notable elevation of the HO−1 content in skin tissues, together with the greatest downregulation of TNF-α, IL-1β, and COX-2. Conclusions: The distinguishing features of Lyc-Se-NPs reveal that this unique compound is a promising antibacterial, antioxidant, and anti-inflammatory agent.