bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental and Numerical Study of the Laminar Burning Velocity and Pollutant Emissions of the Mixture Gas of Methane and Carbon Dioxide
Authors: Wang Yalin , Wang Yu , Zhang Xueqian , Zhou Guoping , Yan Beibei , Bastiaans Rob J. M.
Published: February 2022

This paper presents the experimental and numerical study of the laminar burning velocity and pollutant emissions of the mixture gas of methane and carbon dioxide. Compared to previous research, a wider range of experimental conditions was realized in this paper: CO2 dilution level up to 60% (volume fraction) and equivalence ratio of 0.7–1.3. The burning velocities were measured using the heat flux method. The CO and NO emissions after premixed combustion were measured by a gas analyzer placed 20 cm downstream of the flame. The one-dimensional free flames were simulated using the in-house laminar flame code CHEM1D. Four chemical kinetic mechanisms, GRI-Mech 3.0, San Diego, Konnov, and USC Mech II were used in Chem1D. The results showed that, for laminar burning velocity, the simulation results are all lower than the experimental results. GRI Mech 3.0 showed the best agreement when the CO2 content was below 20%. USC Mech II showed the best consistency when the CO2 content was between 40 and 60%. For CO emission, these four mechanisms all showed a small error compared with the experiments. When CO2 content is higher than 40%, the deviation between simulation and experiment becomes bigger. When the CO2 ratio is more than 20%, the proportion of CO2 does not affect CO emission so much. For NO emission, when the CO2 content is 40%, the results from simulation and experiment showed a good agreement. As the proportion of CO2 increases, the difference in NO emissions decreases.
Mercury Exposure and Its Health Effects in Workers in the Artisanal and Small-Scale Gold Mining (ASGM) Sector—A Systematic Review
Authors: Taux Kira , Kraus Thomas , Kaifie Andrea
Published: February 2022

Gold is one of the most valuable materials but is frequently extracted under circumstances that are hazardous to artisanal and small-scale gold miners’ health. A common gold extraction method uses liquid mercury, leading to a high exposure in workers. Therefore, a systematic review according to the PRISMA criteria was conducted in order to examine the health effects of occupational mercury exposure. Researching the databases PubMed®, EMBASE® and Web of ScienceTM yielded in a total of 10,589 results, which were screened by two independent reviewers. We included 19 studies in this review. According to the quantitative assessment, occupational mercury exposure may cause a great variety of signs and symptoms, in particular in the field of neuro-psychological disorders, such as ataxia, tremor or memory problems. However, many reported symptoms were largely unspecific, such as hair loss or pain. Most of the included studies had a low methodological quality with an overall high risk of bias rating. The results demonstrate that occupational mercury exposure seriously affects miners’ health and well-being.
Reflections on Experiencing Parental Bereavement as a Young Person: A Retrospective Qualitative Study
Authors: Chater Angel Marie , Howlett Neil , Shorter Gillian W. , Zakrzewski-Fruer Julia K. , Williams Jane
Published: February 2022

Background: It is estimated that approximately 41,000 children and young people experience the death of a parent each year. Grief responses, such as anxiety and depression, can follow. This research investigated the adult reflections of experiencing parental death as a young person. Methods: Semi-structured interviews were conducted with adults (N = 14; female n = 8) who experienced parental death as a young person, which occurred over 5 years ago (time since death, M = 12.9 years; age at death, M = 16.4 years; age at interview, M = 30.9 years). The data were analysed inductively using thematic analysis. Results: Seven themes revealed that parental bereavement can lead to (1) “Distance and isolation” and is an (2) “Emotional journey” with (3) a “Physical impact”. Many experienced (4) “Post-traumatic growth” but acknowledged that (5) “Life will never be the same”, highlighting the importance of (6) “Support and understanding” and triggers for (7) “Re-grief”. Conclusions: Parental bereavement has significant emotional and physical consequences, but can also lead to personal growth. Talking therapies were rarely accessed, often due to a lack of awareness or desire to engage, revealing a translational gap between existing support services and uptake. Enabling open conversations about grief and identifying suitable support is a public health priority. This need has been amplified since the start of the COVID-19 pandemic, which may be a trigger for grief empathy and re-grief in those who have already been bereaved.
Pathogenic Biallelic Mutations in ECHS1 in a Case with Short-Chain Enoyl-CoA Hydratase (SCEH) Deficiency-Case Report and Literature Review
Authors: Muntean Carmen , Tripon Florin , Bogliș Alina , Bănescu Claudia
Published: February 2022

ECHS1 gene mutations are known to cause mitochondrial short-chain enoyl-CoA hydratase 1 deficiency, a neurodegenerative disorder characterized by psychomotor development delay, lactic acidosis, and basal ganglia lesions resembling Leigh syndrome. Short-chain enoyl-CoA hydratase 1 (ECHS1) deficiency is a very rare and new disorder, with a wide phenotypic spectrum and different outcomes ranging from neonatal death to survival into adulthood. Since the identification of ECHS1 deficiency in 2014, almost 63 patients with pathogenic mutations in the ECHS1 gene have been described to date. This paper focuses on the clinical and molecular findings as well as the evolution of a Caucasian girl diagnosed with ECHS1 deficiency who carries a new compound heterozygous mutation in the ECHS1 gene. Polymorphic symptoms, namely failure to thrive, significant global developmental delay/regression, movement disorders, ocular abnormalities, hearing loss, seizure, and cardiac myopathy, may be a challenge in mitochondrial disorder suspicion. Early diagnosis, an appropriate diet with valine restriction, and trigger avoidance are essential, as there is no effective therapy for the disease. This disorder influences life quality in these patients and their caregivers, and it has the potential to be fatal.
The Average Man Does Not Exist
Authors: Molenbroek Johan
Published: February 2022

Public health will improve if the prevention means take care of the variation in human characteristics and do not only consider the average or reference man [...]
A Supplementary Approach for Effective Anti-Doping Education: A Pilot Study Applying Refutation Texts to Modify Misperception of the Whereabouts System
Authors: Deng Zhangyan , Guo Jinyang , Wang Dong , Chen Zuosong
Published: February 2022

Background: Over the past twenty years, a multifaceted anti-doping system was established to detect, deter, and prevent doping among athletes. However, perception of the whereabouts system has been a controversial issue. This pilot study aimed to evaluate the effects of refutation text intervention on the perception of the whereabouts system. Methods: In two studies, we tested whether (1) detailed refutation texts are perceived as more effective than simply refuting with a true or false claim among 132 athletes (47.73% female, mean age = 20.99 ± 2.11), and if (2) refutation text intervention can alter the perception of the whereabouts system among 177 athletes (53.11% female, mean age = 21.17 ± 2.27). Descriptive statistics were calculated, followed by a one-sample T-test, independent T-test, chi-square test, and a repeated-measures analysis of variance. Results: The results demonstrate that five true/false statements were developed as refutation texts, and the mean accuracy of the true/false test is less than the probability of guess (p < 0.05, d = −0.18). In addition, detailed refutation texts evoked significantly greater perceived effectiveness than the simple refutation texts (p < 0.01, d = 0.66). Furthermore, the refutation text intervention enhanced the positive perception of the whereabouts system (p < 0.01, η2 = 0.15). Conclusions: Our findings support the efficacy of refutation texts to improve the misperception of anti-doping regimes among athletes and have implications for future education prevention initiatives.
Review of Novel Potential Insulin Resistance Biomarkers in PCOS Patients—The Debate Is Still Open
Authors: Kruszewska Jagoda , Laudy-Wiaderny Hanna , Kunicki Michał
Published: February 2022

Research on proteins and peptides that play roles in metabolic regulation, which may be considered potential insulin resistance markers in some medical conditions, such as diabetes mellitus, obesity and polycystic ovarian syndrome (PCOS), has recently gained in interest. PCOS is a common endocrine disorder associated with hyperandrogenemia and failure of ovulation, which is often accompanied by metabolic abnormalities, including obesity, dyslipidemia, hyperinsulinemia, and insulin resistance. In this review, we focus on less commonly known peptides/proteins and investigate their role as potential biomarkers for insulin resistance in females affected by PCOS. We summarize studies comparing the serum fasting concentration of particular agents in PCOS individuals and healthy controls. Based on our analysis, we propose that, in the majority of studies, the levels of nesfastin-1, myonectin, omentin, neudesin were decreased in PCOS patients, while the levels of the other considered agents (e.g., preptin, gremlin-1, neuregulin-4, xenopsin-related peptide, xenin-25, and galectin-3) were increased. However, there also exist studies presenting contrary results; in particular, most data existing for lipocalin-2 are inconsistent. Therefore, further research is required to confirm those hypotheses, as well as to elucidate the involvement of these factors in PCOS-related metabolic complications.
Good Care during COVID-19: A Narrative Approach to Care Home Staff’s Experiences of the Pandemic
Authors: Dohmen Marleen D. W. , van den Eijnde Charlotte , Thielman Christina L. E. , Lindenberg Jolanda , Huijg Johanna M. , Abma Tineke A.
Published: February 2022

Due to its major impact on Dutch care homes for older people, the COVID-19 pandemic has presented care staff with unprecedented challenges. Studies investigating the experiences of care staff during the COVID-19 pandemic have shown its negative impact on their wellbeing. We aimed to supplement this knowledge by taking a narrative approach. We drew upon 424 personal narratives written by care staff during their work in a Dutch care home during the COVID-19 pandemic. Firstly, our results show that care staff have a relational-moral approach to good care. Residents’ wellbeing is their main focus, which they try to achieve through personal relationships within the triad of care staff–resident–significant others (SOs). Secondly, our results indicate that caregivers experience the COVID-19 mitigation measures as obstructions to relational-moral good care, as they limit residents’ wellbeing, damage the triadic care staff–residents–SOs relationship and leave no room for dialogue about good care. Thirdly, the results show that care staff experiences internal conflict when enforcing the mitigation measures, as the measures contrast with their relational-moral approach to care. We conclude that decisions about mitigation measures should be the result of a dialogic process on multiple levels so that a desired balance between practical good care and relational-moral good care can be determined.
Prolonged Sleep Latency and Reduced REM Latency Are Associated with Depressive Symptoms in a Japanese Working Population
Authors: Omichi Chie , Kadotani Hiroshi , Sumi Yukiyoshi , Ubara Ayaka , Nishikawa Kohei , Matsuda Arichika , Ozeki Yuji ,   the NinJaSleep Study Group
Published: February 2022

Background: Examining the relationship between sleep and depression may be important for understanding the aetiology of affective disorders. Most studies that use electroencephalography (EEG) to objectively assess sleep have been conducted using polysomnography in the laboratory. Impaired sleep continuity, including prolonged sleep latency and changes in rapid eye movement (REM) sleep, have been reported to be associated with depression in clinical settings. Here, we aimed to use home EEG to analyse the association between sleep and depressive symptoms. Methods: We performed a cross-sectional epidemiological study in a large Japanese working population to identify the EEG parameters associated with depressive symptoms based on the results of a questionnaire survey and home EEG measurements using 1-channel (1-Ch) EEG. Results: The study included 650 Japanese patients (41.2% male, 44.7 ± 11.5 years) who underwent home EEG monitoring along with the Patient Health Questionnaire-9 (PHQ-9) to assess depressive symptoms. Logistic regression analysis revealed that depressive symptoms (PHQ-9 ≥ 10) were associated with sleep latency (odds ratio (OR) 1.02; 95% confidence interval (CI): 1.00–1.04) and REM latency (OR, 0.99; 95% CI: 0.99–1.00). Conclusions: Our results suggest that depressive symptoms are associated with prolonged sleep latency and reduced REM latency in a Japanese working population. The 1-Ch EEG may be a useful tool to monitor sleep and screen depression/depressive symptoms in non-clinical settings.
Group Triple P Intervention Effects on Children and Parents: A Systematic Review and Meta-Analysis
Authors: Nogueira Sandra , Canário Ana Catarina , Abreu-Lima Isabel , Teixeira Pedro , Cruz Orlanda
Published: February 2022

Supporting parents through the delivery of evidence-based parenting interventions (EBPI) is a way of promoting children’s rights, given the known benefits to child development and family wellbeing. Group Triple P (GTP) is an EBPI suitable for parents of children aged 2–12 years, who experience parenting difficulties, and/or child behavior problems. Even though GTP has been intensively studied, information lacks on the magnitude of its effects, considering the risk of bias within and across prior research. To address this, a systematic review and meta-analysis (PROSPERO registration CRD42019085360) to evaluate the effects of GTP on child and parent outcomes at short- and longer-term was performed. Through a systematic search of a set of databases, 737 research papers were identified, and 11 trials were selected. The risk of bias within and across studies was evaluated. Significant positive effects of GTP were found immediately after the intervention for child behavior problems, dysfunctional parenting practices, parenting sense of competence, psychological adjustment, parental stress levels, conflict, and relationship quality. Six months after the intervention, positive effects were found only for child behavior problems. Data suggest that GTP might be an effective EBPI leading to positive family outcomes. Substantial risk of bias was found, highlighting the importance of improving the quality of research.
Role Identity, Dissonance, and Distress among Paramedics
Authors: Mausz Justin , Donnelly Elizabeth Anne , Moll Sandra , Harms Sheila , McConnell Meghan
Published: February 2022

Role identity theory describes the purpose and meaning in life that comes, in part, from occupying social roles. While robustly linked to health and wellbeing, this may become unideal when an individual is unable to fulfill the perceived requirements of an especially salient role in the manner that they believe they should. Amid high rates of mental illness among public safety personnel, we interviewed a purposely selected sample of 21 paramedics from a single service in Ontario, Canada, to explore incongruence between an espoused and able-to-enact paramedic role identity. Situated in an interpretivist epistemology and using successive rounds of thematic analysis, we developed a framework for role identity dissonance wherein chronic, identity-relevant disruptive events cause emotional and psychological distress. While some participants were able to recalibrate their sense of self and understanding of the role, for others, this dissonance was irreconcilable, contributing to disability and lost time from work. In addition to contributing a novel perspective on paramedic mental health and wellbeing, our work also offers a modest contribution to the theory in using the paramedic context as an example to consider identity disruption through chronic workplace stress.
Inclusive Aging in Korea: Eradicating Senior Poverty
Authors: Kang Jooyeon , Park Jungmin , Cho Jaemin
Published: February 2022

Poverty for the elderly is one of the most urgent social problems when discussing the social problems facing Korean society. The purpose of this study is to identify the causes of elderly poverty problems and to seek countermeasures. According to a systematic analysis of the economic difficulties of the elderly population that applies a socioecological model, the cause of elderly poverty is complicated by the specificity of the labor market and pension system in Korean society. This is compounded by the lack of a public support system that can overcome insufficient family care and a lack of individual preparation. To alleviate elderly poverty, this paper recommends three policy alternatives. First, a robust multipillar retirement income security system must be established. To secure a minimal retirement income for the elderly in poverty, who have been marginalized from the public pension system design, the basic pension should be raised for the bottom 70% of senior citizens. Second, in order to tackle labor market duality and early retirement, the seniority-oriented wage system should be reformed into a job-based wage system. Third, to minimize unemployment and promote quality among re-employment jobs, the government should strengthen vocational skills development by expanding programs tailored to older people.
A Method to Identify Urban Fringe Area Based on the Industry Density of POI
Authors: Dong Qi , Qu Shuxue , Qin Jiahui , Yi Disheng , Liu Yusi , Zhang Jing
Published: February 2022

During the period of rapid urbanization, the urban fringe area is the area where urban expansion occurs first, and land use change is the most active. Studying its evolution laws and characteristics is of great significance to urban planning and urban expansion, and the primary task of fringe area research is the spatial recognition and boundary division of urban fringe area. The previous methods for defining urban fringe areas are mainly divided into qualitative division based on experience and quantitative division based on indicators constructing. This research avoids the construction of index systems and the selection of mathematical models and improves the objectivity of the experiment. Based on the existing methods, this research considers the correlation between the difference of industrial distribution within cities and the urban spatial structure and spatial distribution of urban elements and considers the distance decay law of urban density. The urban fringe area in this research is defined as the distinction region of the service and manufacturing industry extending outward from the inside of the city. First, calculate the POI density of service industry and manufacturing industry. Then look for the inflection point where its density value drops sharply and get the isoline of that point. The range within the isoline is that the industry extends outward from the inner city and has reached the saturation state. Two types of industries can determine two isolines, and the belt region between those isolines is the urban fringe area. We use the urban fringe area identified from the impervious surface data to verify the result. The comparative results show that the identification method of urban fringe area based on POI works effectively, and it can successfully identify the multi-center urban core area. The method mentioned in this paper provides a new idea from the perspective of industrial activities in identifying and defining the belt region of urban fringe area.
Topological Relationships Cytoskeleton-Membrane Nanosurface-Morphology as a Basic Mechanism of Total Disorders of RBC Structures
Authors: Kozlova Elena , Sergunova Viktoria , Sherstyukova Ekaterina , Gudkova Olga , Kozlov Aleksandr , Inozemtsev Vladimir , Lyapunova Snezhanna , Chernysh Aleksandr
Published: February 2022

The state of red blood cells (RBCs) and their functional possibilities depend on the structural organization of the membranes. Cell morphology and membrane nanostructure are compositionally and functionally related to the cytoskeleton network. In this work, the influence of agents (hemin, endogenous oxidation during storage of packed RBCs, ultraviolet (UV) radiation, temperature, and potential of hydrogen (pH) changes) on the relationships between cytoskeleton destruction, membrane nanostructure, and RBC morphology was observed by atomic force microscope. It was shown that the influence of factors of a physical and biochemical nature causes structural rearrangements in RBCs at all levels of organization, forming a unified mechanism of disturbances in relationships “cytoskeleton-membrane nanosurface-cell morphology”. Filament ruptures and, consequently, large cytoskeleton pores appeared. The pores caused membrane topological defects in the form of separate grain domains. Increasing loading doses led to an increase in the number of large cytoskeleton pores and defects and their fusion at the membrane nanosurfaces. This caused the changes in RBC morphology. Our results can be used in molecular cell biology, membrane biophysics, and in fundamental and practical medicine.
Novel Oleanane-Type Triterpene Glycosides from the Saponaria officinalis L. Seeds and Apoptosis-Inducing Activity via Mitochondria
Authors: Takahashi Naoki , Iguchi Tomoki , Kuroda Minpei , Mishima Masaki , Mimaki Yoshihiro
Published: February 2022

Saponaria officinalis L., commonly known as “Soapwort”, is a rich source of triterpene glycosides; however, the chemical constituents of S. officinalis seeds have not been fully identified. In this study, we conducted a systematic phytochemical investigation of the seeds of S. officinalis and obtained 17 oleanane-type triterpene glycosides (1–17), including seven new glycosides (1–7). The structures of 1–7 were determined based on a detailed analysis of NMR spectroscopic data and chromatographic and spectroscopic analyses following specific chemical transformation. The cytotoxicities of the isolated compounds were evaluated against HL-60 human promyelocytic leukemia cells, A549 human adenocarcinoma lung cancer cells, and SBC-3 human small-cell lung cancer cells. The cytotoxicities of 1, 4, and 10 toward HL-60 cells and SBC-3 cells were nearly as potent as that of cisplatin. Compound 1, a bisdesmosidic triterpene glycoside obtained in good yield, arrested the cell cycle of SBC-3 cells at the G2/M phase, and induced apoptosis through an intrinsic pathway, accompanied by ROS generation. As a result of the mitochondrial dysfunction induced by 1, mitochondria selective autophagy, termed mitophagy, occurred in SBC-3 cells.
Whole Body Ip6k1 Deletion Protects Mice from Age-Induced Weight Gain, Insulin Resistance and Metabolic Dysfunction
Authors: Ghoshal Sarbani , Mukherjee Sandip , Chakraborty Molee , Msengi Eliwaza Naomi , Haubner Jake , Chakraborty Anutosh
Published: February 2022

(1) Background: We previously demonstrated that disruption of IP6K1 improves metabolism, protecting mice from high-fat diet-induced obesity, insulin resistance, and non-alcoholic fatty liver disease and steatohepatitis. Age-induced metabolic dysfunction is a major risk factor for metabolic diseases. The involvement of IP6K1 in this process is unknown. (2) Methods: Here, we compared body and fat mass, insulin sensitivity, energy expenditure and serum-, adipose tissue- and liver-metabolic parameters of chow-fed, aged, wild type (aWT) and whole body Ip6k1 knockout (aKO) mice. (3) Results: IP6K1 was upregulated in the adipose tissue and liver of aWT mice compared to young WT mice. Moreover, Ip6k1 deletion blocked age-induced increase in body- and fat-weight and insulin resistance in mice. aKO mice oxidized carbohydrates more efficiently. The knockouts displayed reduced levels of serum insulin, triglycerides, and non-esterified fatty acids. Ip6k1 deletion partly protected age-induced decline of the thermogenic uncoupling protein UCP1 in inguinal white adipose tissue. Targets inhibited by IP6K1 activity such as the insulin sensitivity- and energy expenditure-inducing protein kinases, protein kinase B (PKB/Akt) and AMP-activated protein kinase (AMPK), were activated in the adipose tissue and liver of aKO mice. (4) Conclusions: Ip6k1 deletion maintains healthy metabolism in aging and thus, targeting this kinase may delay the development of age-induced metabolic dysfunction.
A Survey on Text Classification Algorithms: From Text to Predictions
Authors: Gasparetto Andrea , Marcuzzo Matteo , Zangari Alessandro , Albarelli Andrea
Published: February 2022

In recent years, the exponential growth of digital documents has been met by rapid progress in text classification techniques. Newly proposed machine learning algorithms leverage the latest advancements in deep learning methods, allowing for the automatic extraction of expressive features. The swift development of these methods has led to a plethora of strategies to encode natural language into machine-interpretable data. The latest language modelling algorithms are used in conjunction with ad hoc preprocessing procedures, of which the description is often omitted in favour of a more detailed explanation of the classification step. This paper offers a concise review of recent text classification models, with emphasis on the flow of data, from raw text to output labels. We highlight the differences between earlier methods and more recent, deep learning-based methods in both their functioning and in how they transform input data. To give a better perspective on the text classification landscape, we provide an overview of datasets for the English language, as well as supplying instructions for the synthesis of two new multilabel datasets, which we found to be particularly scarce in this setting. Finally, we provide an outline of new experimental results and discuss the open research challenges posed by deep learning-based language models.
A Study on the Effect of Customer Habits on Revisit Intention Focusing on Franchise Coffee Shops
Authors: Lee Hong-Joo
Published: February 2022

As the economy grows in South Korea and the needs of consumers become increasingly diversified, various consumer markets are emerging. In this study, the reasons for customers’ intentions to revisit a franchise coffee shop were explored. Many researchers have found that customer satisfaction is a major factor in the intention to revisit, and the factors affecting this satisfaction have been presented as major research topics. However, in this study, in order to examine the consumers’ behavior toward revisit intentions, the results were analyzed by dividing the consumer data into a group of college students in their 20s and a group of office workers in their 30s and 40s. The results of this study found that in regard to customers’ habitual intentions to revisit, perceived product quality and brand awareness were more influential than service quality or the physical environment. In particular, before returning to a coffee shop, customers habitually recalled the coffee shop they wanted to revisit, suggesting that the taste of the coffee and the quality of the various products had a very important impact on their revisiting habits.
A Privacy-Preserving and Standard-Based Architecture for Secondary Use of Clinical Data
Authors: Ciampi Mario , Sicuranza Mario , Silvestri Stefano
Published: February 2022

The heterogeneity of the formats and standards of clinical data, which includes both structured, semi-structured, and unstructured data, in addition to the sensitive information contained in them, require the definition of specific approaches that are able to implement methodologies that can permit the extraction of valuable information buried under such data. Although many challenges and issues that have not been fully addressed still exist when this information must be processed and used for further purposes, the most recent techniques based on machine learning and big data analytics can support the information extraction process for the secondary use of clinical data. In particular, these techniques can facilitate the transformation of heterogeneous data into a common standard format. Moreover, they can also be exploited to define anonymization or pseudonymization approaches, respecting the privacy requirements stated in the General Data Protection Regulation, Health Insurance Portability and Accountability Act and other national and regional laws. In fact, compliance with these laws requires that only de-identified clinical and personal data can be processed for secondary analyses, in particular when data is shared or exchanged across different institutions. This work proposes a modular architecture capable of collecting clinical data from heterogeneous sources and transforming them into useful data for secondary uses, such as research, governance, and medical education purposes. The proposed architecture is able to exploit appropriate modules and algorithms, carry out transformations (pseudonymization and standardization) required to use data for the second purposes, as well as provide efficient tools to facilitate the retrieval and analysis processes. Preliminary experimental tests show good accuracy in terms of quantitative evaluations.
Morphological Structure and Distribution of Hairiness on Different Body Parts of Apis mellifera with an Implication on Pollination Biology and a Novel Method to Measure the Hair Length
Authors: Khan Kamal Ahmed , Liu Tengteng
Published: February 2022

Bees play a very important role in pollination, especially western honey bees, which contribute upwards of billions of dollars concerning crop pollination. Hairiness plays an important role in pollination success by transporting pollen, and pollen intake, but there is a lack of detailed studies on the morphological mechanisms. The hairiness trait is barely discussed in pollinator trait analysis because of the lack of systematic techniques used to measure hairiness. This paper reports a novel method that is used to measure the hair length of different body parts of a western honey bee through a stereomicroscope equipped with live measurement module software. Scanning electron microscope (SEM) was used to update the knowledge regarding the hair structure of a western honey bee. We explained different types of hairs, hair branches, and their distributions on different body parts, which are discussed in detail. A positive correlation was found between hair length and the number of branches on all body parts. Five types of branches were observed, and these branches vary with different body parts. Our study provides sufficient details about the hair morphology of the western honey bee and a new methodology for measuring hair length. This methodology will improve the knowledge about understanding the pollination efficiency of the western honey bee.
Organophosphate Insecticides Resistance in Field Populations of House Flies, Musca domestica L.: Levels of Resistance and Acetylcholinesterase Activity
Authors: Abobakr Yasser , Al-Hussein Faisal I. , Bayoumi Alaa E. , Alzabib Ali A. , Al-Sarar Ali S.
Published: February 2022

The house fly, Musca domestica L., is an important medical and veterinary pest associated with humans and livestock. Management of house flies has relied extensively on chemical control. In this study, we report on the resistance of house fly field-collected populations to diazinon and fenitrothion OP insecticides in Riyadh, Saudi Arabia. The diazinon and fenitrothion median lethal dose (LD50) values against adult female M. domestica field-collected populations were significantly higher than those of the laboratory (LAB) strain. Different levels of resistance were detected in all field-collected populations toward the two OP insecticides. The resistance ratios for diazinon ranged from 62.47 to 309.78, while there were 53.08 to 261.24 for fenitrothion in the eight field-collected populations. The specific activity of acetylcholinesterase (AChE) in all field populations was significantly (p < 0.05) higher than that in the LAB strain. In vitro diazinon and fenitrothion median inhibitory concentration (IC50) values of LAB strain AChE activity were significantly (p < 0.05) lower than those for field-collected populations. This study found high levels of resistance in the house fly field-collected populations to diazinon and fenitrothion. Replacing these two insecticides and any other OPs with novel ones that have different modes of action is an urgent need in the insect-vector control programs in Riyadh, Saudi Arabia. An altered AChE enzyme of M. domestica field populations might be partially responsible for the developed resistance. Monitoring of insecticide resistance development in M. domestica populations and a better understanding of its mechanisms are needed to design operative management strategies for controlling the house flies.
1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees
Authors: Lee Saetbyeol , Kalcic Filip , Duarte Iola , Titera Dalibor , Kamler Martin , Mrna Pavel , Hyrsl Pavel , Danihlik Jiri , Dobes Pavel , Kunc Martin , Pudlo Anna , Havlik Jaroslav
Published: February 2022

In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze the metabolic differences between summer and winter bees of the same age. The multivariate analysis showed that summer and winter bees could be distinguished based on their metabolic profiles. Among the 36 metabolites found, 28 metabolites have displayed significant changes from summer to winter bees. Compared to summer bees, trehalose in winter bees showed 1.9 times higher concentration, and all amino acids except for proline and alanine showed decreased patterns. We have also detected an unknown compound, with a CH3 singlet at 2.83 ppm, which is a potential biomarker that is about 13 times higher in summer bees. Our results show that the metabolites in summer and winter bees have distinctive characteristics; this information could provide new insights and support further studies on honey bee longevity and overwintering.
Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia
Authors: Pramasivan Sandthya , Liew Jonathan Wee Kent , Jeyaprakasam Nantha Kumar , Low Van Lun , Ngui Romano , Vythilingam Indra
Published: February 2022

The Leucosphyrus Group of mosquitoes are the major simian malaria vectors in Malaysia. Accurate species identification is required to help in curbing the spread of simian malaria. The aim of the study is to provide an accurate molecular method for identifying the four important Anopheles vector species found in Malaysia. Mosquito specimens were collected from various localities in Malaysia, where simian malaria cases were reported. DNA from 122 mosquito specimens was tested to develop a multiplex polymerase chain reaction (PCR) assay. The specificity of this assay was tested against other mosquito species. Molecular identification of the species was further confirmed by analysing the internal transcribed spacer 2 (ITS2) DNA region of the specimens. Anopheles balabacensis and An. latens showed two distinct clades in the phylogenetic tree. The multiplex PCR assay was developed based on the ITS2 region for the identification of Anopheles introlatus (298–299 bp), Anopheles latens (197–198 bp), Anopheles cracens (421–426 bp), and Anopheles balabacensis (224–228 bp). This method will be useful to accurately identify the major Anopheles Leucosphyrus Group species in Malaysia, which are difficult to identify morphologically, to determine the correct vector as well as its geographical distribution.
The Development of SiPM-Based Fast Time-of-Flight Detector for the AMS-100 Experiment in Space
Authors: Chung Chanhoon , Backes Theresa , Dittmar Clemens , Karpinski Waclaw , Kirn Thomas , Louis Daniel , Schwering Georg , Wlochal Michael , Schael Stefan
Published: February 2022

AMS-100 is the next-generation high-energy cosmic-ray experiment in Space. It is designed as a magnetic spectrometer with a geometrical acceptance of 100 m2 · sr to be operated for ten years at the Sun–Earth Lagrange Point 2. Its Time-of-Flight (TOF) detector is a crucial sub-detector for the main trigger and the particle identification constructed from individual scintillation counters. A fast time measurement with a resolution of 20 ps for a single counter is required to cover wide energy ranges for particle identification. A prototype counter has been designed based on a fast plastic scintillator tile readout by two silicon photomultipliers (SiPMs). An amplifier board was built to merge 16 SiPM channels into four readout channels in a hybrid connection. The signals are read out by a fast waveform digitizer. The timing performance was studied with electrons from a 90Sr source. A time resolution of 40 ps for a single counter has been achieved. Various operational and environmental conditions have been studied.
Suicidal Ideation and Suicide-Attempt-Related Hospitalizations among People with Alzheimer’s Disease (AD) and AD-Related Dementias in the United States during 2016–2018
Authors: Alipour-Haris Golnoosh , Armstrong Melissa , Sullivan Jennifer , Suryadevara Uma , Rouhizadeh Masoud , Brown Joshua
Published: February 2022

People living with Alzheimer’s disease (AD) and AD-related dementias (ADRDs) are at a higher risk of suicidal behaviors given intersecting risk factors. Previous studies generally only focused on AD, small clinical samples, or grouped all dementia subtypes together, limiting insights for other ADRD subtypes. The objective of this study was to generate evidence related to the relative burden of suicidal behaviors (suicidal ideation and suicide attempt) among people with AD and ADRDs. This retrospective cross-sectional study identified hospitalizations related to suicidal behaviors (suicidal ideation and suicide attempt) for patients with Alzheimer’s disease (AD) and AD-related dementias using ICD-10-CM codes from the Nationwide Readmissions Database (NRD). A logistic regression model was estimated to assess associations between AD/ADRD subtype and patient characteristics, and the risk for a suicidal-behavior-related hospitalization and modes of harm were reported. During 2016–2018, there were 12,538 hospitalizations related to suicidal behaviors for people with AD/ADRDs. The overall prevalence of suicidal-behavior-related hospitalizations was lowest for AD (0.8%) and highest for frontotemporal dementia (2.6%). Among hospitalizations for suicide attempts, the most common mode of harm was medications or drugs (89.2% of all attempts), followed by weapons (17.7%). We found that there was a difference in the frequency of suicidal-behavior-related hospitalizations among AD/ADRD hospitalized patients across dementia subtypes.
Incidence, Impact and Treatment of Ongoing CMV Infection in Patients with Biliary Atresia in Four European Centres
Authors: Fischler Björn , Czubkowski Piotr , Dezsofi Antal , Liliemark Ulrika , Socha Piotr , Sokol Ronald J. , Svensson Jan F. , Davenport Mark
Published: February 2022

Cytomegalovirus (CMV) infection has been suggested to be of importance for the development and outcome of biliary atresia (BA). However, most data are only available from single centre studies. We retrospectively collected data on rates, outcomes, and treatments for ongoing CMV infection at the time of Kasai portoenterostomy (KPE) from four different tertiary centres in Europe. The rate of ongoing CMV infection varied between 10–32% in the four centres. CMV positive patients were significantly older and had higher levels of several liver biochemistries at the time of KPE (p < 0.05 for all comparisons). In the largest centre, CMV infection was more common in non-Caucasians, and CMV infected patients had poorer long-term survival with native liver than CMV negative patients (p = 0.0001). In contrast, survival with native liver in the subgroup of CMV infected patients who had received antiviral treatment was similar to the CMV negative group. We conclude that ongoing CMV infection at the time of KPE occurs in a significant proportion of BA patients and that these patients seem to differ from CMV negative patients regarding age and biochemistry at the time of KPE as well as long-term survival with native liver. The latter difference may be reduced by antiviral treatment, but randomized, controlled trials are needed before such treatment can be recommended routinely.
Prognostic Value of Decreased High-Density Lipoprotein Cholesterol Levels in Infective Endocarditis
Authors: Zampino Rosa , Patauner Fabian , Karruli Arta , Iossa Domenico , Ursi Maria Paola , Bertolino Lorenzo , Peluso Anna Maria , D’Amico Fabiana , Cavezza Giusi , Durante-Mangoni Emanuele
Published: February 2022

(1) Background: Simple parameters to be used as early predictors of prognosis in infective endocarditis (IE) are lacking. The aim of this study was to evaluate the prognostic role of high-density-lipoprotein cholesterol (HDL-C) and also of total-cholesterol (TC), low-density-lipoprotein cholesterol (LDL-C), and triglycerides, in relation to clinical features and mortality, in IE. (2) Methods: Retrospective analysis of observational data from 127 consecutive patients with a definite diagnosis of IE between 2016 and 2019. Clinical, laboratory and echocardiography data, mortality, and co-morbidities were analyzed in relation to HDL-C and lipid profile. (3) Results: Lower HDL-C levels (p = 0.035) were independently associated with in-hospital mortality. HDL-C levels were also significantly lower in IE patients with embolic events (p = 0.036). Based on ROC curve analysis, a cut-off value was identified for HDL-C equal to 24.5 mg/dL for in-hospital mortality. HDL-C values below this cut-off were associated with higher triglyceride counts (p = 0.008), higher prevalence of S. aureus etiology (p = 0.046) and a higher in-hospital mortality rate (p = 0.004). Kaplan–Meier survival analysis showed higher 90-day mortality in patients with HDL-C ≤ 24.5 mg/dL (p = 0.001). (4) Conclusions: Low HDL-C levels could be used as an easy and low-cost marker of severity in IE, particularly to predict complications, in-hospital and 90-day mortality.
A Prospective Investigation of Tumor Hypoxia Imaging with 68Ga-Nitroimidazole PET/CT in Patients with Carcinoma of the Cervix Uteri and Comparison with 18F-FDG PET/CT: Correlation with Immunohistochemistry
Authors: Mokoala Kgomotso M. G. , Lawal Ismaheel O. , Maserumule Letjie C. , Hlongwa Khanyisile N. , Ndlovu Honest , Reed Janet , Bida Meshack , Maes Alex , van de Wiele Christophe , Mahapane Johncy , Davis Cindy , Jeong Jae Min , Popoola Gbenga , Vorster Mariza , Sathekge Mike M.
Published: February 2022

Hypoxia in cervical cancer has been associated with a poor prognosis. Over the years 68Ga labelled nitroimidazoles have been studied and have shown improved kinetics. We present our initial experience of hypoxia Positron Emission Tomography (PET) imaging in cervical cancer with 68Ga-Nitroimidazole derivative and the correlation with 18F-FDG PET/CT and immunohistochemistry. Twenty women with cervical cancer underwent both 18F-FDG and 68Ga-Nitroimidazole PET/CT imaging. Dual-point imaging was performed for 68Ga-Nitroimidazole PET. Immunohistochemical analysis was performed with hypoxia inducible factor-1α (HIF-1α). We documented SUVmax, SUVmean of the primary lesions as well as tumor to muscle ratio (TMR), tumor to blood (TBR), metabolic tumor volume (MTV) and hypoxic tumor volume (HTV). There was no significant difference in the uptake of 68Ga-Nitroimidazole between early and delayed imaging. Twelve patients had uptake on 68Ga-Nitroimidazole PET. Ten patients demonstrated varying intensities of HIF-1α expression and six of these also had uptake on 68Ga-Nitroimidazole PET. We found a strong negative correlation between HTV and immunohistochemical staining (r = −0.660; p = 0.019). There was no correlation between uptake on PET imaging and immunohistochemical analysis with HIF-1α. Two-thirds of the patients demonstrated hypoxia on 68Ga-Nitroimidazole PET imaging.
Efficacy and Safety of the Hexanic Extract of Serenoa repens vs. Watchful Waiting in Men with Moderate to Severe LUTS-BPH: Results of a Paired Matched Clinical Study
Authors: Alcaraz Antonio , Gacci Mauro , Ficarra Vincenzo , Medina-Polo José , Salonia Andrea , Fernández-Gómez Jesús , Ciudin Alexandru , Castro-Díaz David , Rodríguez-Antolín Alfredo , Carballido-Rodríguez Joaquín , Cózar-Olmo José , Búcar-Terrades Santiago , Pérez-León Noemí , Brenes-Bermúdez Francisco , Molero-García José , Ledesma Antonio , Herdman Michael , Manasanch José , Angulo Javier , Group on
Published: February 2022

We investigated changes in symptoms and quality of life (QoL) in men with moderate-to-severe lower urinary tract symptoms associated with benign prostatic hyperplasia (LUTS/BPH) receiving the hexanic extract of Serenoa repens (HESr) and compared results with a matched group on watchful waiting (WW). Data was from a real-world, open-label, prospective, multicenter study. This sub-group analysis included patients with moderate-to-severe symptoms receiving either the HESr 320 mg/daily for six months (HESr) or who remained untreated for LUTS/BPH (WW). Changes in urinary symptoms and QoL were measured by IPSS and BII questionnaires. Two statistical approaches (iterative matching and propensity score pairing) were used to maximize between-group comparability at baseline. Tolerability was assessed in the HESr group. After iterative matching, data for analysis was available for 783 patients (102 WW, 681 HESr). IPSS scores improved by a mean (SD) of 3.8 (4.4) points in the HESr group and by 2.2 (4.5) points in the WW group (p = 0.002). Changes in BII score were 1.8 (2.4) points and 1.0 (2.2) points, respectively (p < 0.001). Three patients (0.9%) treated with the HESr reported mild adverse effects. Moderate-severe LUTS/BPH patients treated for six months with the HESr showed greater improvements in symptoms and QoL than matched patients on WW, with a very low rate of adverse effects.
The Effect of Past Cataract Surgery within the Medium to Long-Term Period on Patients with Dry Eye Disease
Authors: Siew Lei , Tong Louis
Published: February 2022

This study investigates the effects of cataract surgery on dry eye parameters of patients with dry eye disease (DED) in the medium- to long-term post-surgical period (6 months to 5 years). A cross-sectional study was conducted on 438 eyes on first visit to a tertiary clinic (219 eyes with cataract surgery within timeframe, i.e., pseudophakic dry eye group, 219 comparison eyes without such history, i.e., comparison dry eye group). Parameters evaluated include Ocular Surface Disease Index (OSDI) and standard examination for DED. A significantly greater proportion of pseudophakic dry eye group (50%) experienced frequent blurred vision (≥1 episode per week) compared to dry eye control group (38%) (OR = 1.66, 95%CI 1.13, 2.44). Those with ocular discomfort before surgery were more likely to experience blurring at least once a day. However, a significantly greater proportion of the pseudophakic dry eye group (34%) had Schirmer’s I >8mm compared to the comparison group (25%) (OR = 0.605, 95% CI 0.398, 0.921), though the difference was not clinically significant (7.51 mm versus 6.51 mm, p > 0.05). Other DED signs (e.g., overall OSDI score, Tear Break-up Time) were not found to be worse among pseudophakic dry eye group. Pre-operative counselling and preventative measures should be undertaken, especially those with suggestive symptoms pre-operatively.