bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALYSIS OF ROAD SAFETY IN THE BALURAN STREET KM SBY 230 - 231 WITH ROAD SAFETY AUDIT
Authors: Azizah Nur , Hasanuddin Akhmad , Sukmawati Sri
Published: May 2020

The Baluran Street Sumberejo Village Banyuputih Districts Situbondo Districts KM SBY 230-231 is categorized as block spot area with 15 accident to 6 dead, 6 people seriously injured (LB) and minor injuries (LR ) 21 people as well as the material loss of Rp113.250.000,00 during the year 2015-2017. The purpose of this study is determined to the factor accident and provided recommendation for handling road safety in the case of traffic accidents. The road safety analysis data used are road safety audit data and accident data released by Unit Laka Lantas Polres Situbondo in 2017. The result of road safety analysis indicates that several road facilities are categorized as “dangerous” and/or “very dangerous” which shall be improved to reduce traffic accident potential. The improvement may includes (1) the geometric aspect of the road, including stop visibility, sight distance to overtake, bend radius, vertical ramp, critical length, vertical bend length, stop visibility at vertical arch, different shoulder elevation road to pavement edge; (2) pavement aspect, including hole and crack; and (3) the complementary aspects of the road including the speed limit at the corner, climbing sign, sign of descendant, anticipatory prohibition sign, bend sign, double bend sign, and road marking.

Jalan Raya Baluran Desa Sumberejo Kecamatan Banyuputih Kabupaten Situbondo KM SBY 230-231 merupakan lokasi rawan kecelakaan yang mengalami 15 kejadian kecelakaan dengan jumlah korban meninggal dunia (MD) sebanyak 6 orang, korban luka berat (LB) 6 orang dan korban luka ringan (LR) 21 orang serta kerugian materi sebesar Rp113.250.000,00 selama tahun 2015-2017. Tujuan penelitian ini yaitu untuk menentukan faktor penyebab kecelakaan dan memberikan rekomendasi penanganan keselamatan jalan pada kasus kecelakaan lalu lintas. Data analisis keselamatan jalan yang digunakan adalah data hasil audit keselamatan jalan dan data kecelakaan yang dikeluarkan oleh Unit Laka Lantas Polres Situbondo tahun 2017. Hasil analisis keselamatan jalan menunjukkan bahwa beberapa bagian fasilitas jalan berada dalam kategori “bahaya” dan atau “sangat berbahaya”, yang harus segera diperbaiki untuk memperkecil potensi terjadinya kecelakaan, yaitu: (1) aspek geometrik jalan yang meliputi jarak pandang henti, jarak pandang menyiap, radius tikungan, landai vertikal, panjang kritis, panjang lengkung vertikal, jarak pandang henti pada lengkung vertikal, beda elevasi bahu jalan terhadap tepi perkerasan; (2) aspek perkerasan jalan yang meliputi kerusakan berupa lubang dan retak; dan (3) aspek pelengkap jalan yang meliputi rambu batas kecepatan di tikungan, rambu tanjakan, rambu turunan, rambu larangan mendahului, rambu tikungan, rambu tikungan ganda, dan marka jalan.
EVALUATION FINAL SETTLEMENT IN FIED UING OBSERVATIONAL METHOD: ASAOKA AND HYPERBOLIC IN PPKA PROJECT SECTION 2 PALEMBANG SOUTH SUMATRA
Authors: Efendi Aflah Baihaqi , Nurtjahtjaningtyas Indra , Wicaksono Luthfi Amri
Published: May 2020

In the construction of the Trans Sumatra Toll Road, the existing land that will be used as a foundation belongs to the soft soil category. This is because the original soil to be built by the toll road is swamps. The soil improvement method used in PPKA section 2 is Vacuum Consolidation. In this study, a geotechnical instrument evaluation was carried out using the Settlement Plate to evaluate the actual settlement, then calculate the final settlement prediction with the Asaoka and Hyperbolic observation methods. Then from the monitoring data, the prediction of the final settlement was calculated using the Asaoka and hyperbolic observation methods, calculated the value of the degree of consolidation from each method. So that the results of the calculation can be concluded in the form of the superiority of each method used as consideration for further planning. The results obtained in this study are the actual settlement with theoretical and settlement predictions along with the comparison of the two observation methods. From the average value of the degree of consolidation produced also in the percentage of 98.68% while the hyperbolic is 99.35%. The calculation of soil subsidence using the Asaoka method is more same with the field (actual). Because the data used for the Asaoka comes from all monitoring data on the field settlement plate.

Pada pembangunan jalan Tol Trans Sumatra, tanah eksisting yang akan dijadikan pondasi tergolong dalam kategori tanah lunak. Hal itu dikarenkan tanah asli yang akan dibangun jalan tol merupakan rawa-rawa. Metode perbaikan tanah yang digunakan di PPKA seksi 2 adalah Vacuum Consolidation. Dalam kajian ini, dilakukan evaluasi instrumen geoteknik menggunakan Settlement Plate untuk mengevaluasi penurunan aktual, kemudian menghitung prediksi penurunan akhir (final settlement) dengan metode observasi Asaoka dan Hiperbolik. Kemudian dari data monitoring dihitung besar prediksi penurunan akhir tanah menggunakan metode observasi asaoka dan hiperbolik, dihitung nilai derajat konsolidasi dari setiap metode. Sehingga dari hasil perhitungan didapat kesimpulan berupa keunggulan setiap metode yang digunakan sebagai pertimbangan untuk perencanaan lanjutan. Hasil yang diperoleh dalam kajian ini adalah besar penurunan aktual dengan teoritis dan prediksi settlement beserta mengetahui perbandingan dari kedua metode observasi. Dari nilai rerata derajat konsolidasi yang dihasilkan juga pada asaoka sebesar 98,68% sedangkan pada hiperbolik sebesar 99,35%. Perhitungan penurunan tanah menggunakan metode asaoka lebih sesuai dengan lapangan (aktual). Dikarenakan data yang digunakan untuk asaoka berasal dari semua data monitoring settlement plate lapangan.
A SCHEDULING ON IMPLEMENTATION OF ISDB PROJECT JEMBER UNIVERSITY WITH PRECEDENCE DIAGRAM METHOD
Authors: Derka Icha Tatrisya , Suyoso Hernu , Ratnaningsih Anik
Published: May 2020

Scheduling is important, because of the time limit, so the implementation of project activities must be planned and timely. The Integrated Laboratory for Natural Science and Food Technology building is one of the development projects of the IsDB (Islamic Development Bank), in the Jember University. In this study, the schedule will be carried out using the PDM method (Precedence Diagram Method), assisted by the Microsoft Project. The PDM is a working network with a clear dependency relationship and can be used on projects that have overlapping activities between tasks to another, PDM can show critical trajectory. Secondary data needed in the form of soft-drawings, BoQ (Bill of Quantity), City of Jember AHS 2018 and S curve. There are 30 jobs included in the critical track including 14 jobs in construction work, 11 jobs in architectural work and 5 jobs in outside work. The results of the study indicate that the duration of the construction Building is 242 days, or 8 months 16 days.

Penjadwalan merupakan hal yang penting, karena adanya batasan waktu, maka pelaksanaan kegiatan proyek harus tepat rencana dan tepat waktu. Gedung Integrated Laboratory for Natural Science and Food Technology merupakan salah satu pembangunan proyek IsDB (Islamic Development Bank), yang ada di lingkungan Universitas Jember. Pada penelitian ini, akan dilakukan penjadwalan menggunakan metode PDM (Precedence Diagram Method) dibantu dengan Microsoft Project. PDM merupakan jaringan kerja dengan hubungan ketergantungan yang jelas dan dapat digunakan pada proyek yang mempunyai kegiatan tumpang tindih antar satu pekerjaan ke pekerjaan lainnya, serta dapat menunjukkan lintasan kritis. Data yang dibutuhkan berupa data sekunder yaitu gambar rencana, BoQ, AHS Kota Jember 2018 dan kurva S. Terdapat 30 pekerjaan yang termasuk dalam lintasan kritis meliputi 14 pekerjaan di pekerjaan strukur, 11 pekerjaan di pekerjaan arsitektur serta 5 pekerjaan di pekerjaan luar bangunan. Hasil penelitian menunjukkan bahwa jangka waktu pelaksanaan pembangunan Gedung Integrated Laboratory for Natural Science and Food Technology adalah selama 242 hari, atau 8 bulan lebih 16 hari.
DEVELOPMENT OF CLEAN WATER DISTRIBUTION NETWORK SYSTEM IN GENTENG SUB-DISTRICT BANYUWANGI USING EPANET 2.0 PROGRAM
Authors: Jayanti Ayu Rahmad , Badriani Ririn Endah , Dhokhikah Yeny
Published: May 2020

The clean water distribution in the Genteng Subdistrict, Banyuwangi Regency is included in the service area of the Zone 1 PDAM tile unit. The 60 liters/second reservoir discharge capacity is obtained from Sumber Umbul Sari in the Glenmore District. The distribution of clean water in Zone 1 is still less than 70% of the area served, as the installed discharge capacity is estimated to be insufficient. In order to achieve the distribution goal, a network system must be developed by adding direct debits and planning a new pipeline. The Epanet 2.0 program simplifies the calculation of pipeline networks by integrating elevation data, network maps, pipeline specification, and load. The analysis of the simulation results was conducted using the Public Works Minister's hydraulic parameter standards 2007. Planning of a distribution network and a cost budget in 2029 were done to estimate the water supply needs and budgets required. The hydraulic simulation results based on the analysis of the pressure of all joints are in accordance with the standard, while the analysis of the velocity in pipe is less standard. The need for water discharge in 2029 is 71.6 liters/second. In Kembiritan Village, the construction of distribution pipes with an additional reservoir unit was planned. The planned pipe dimensions in the development area were 25 mm at 796 meters, 50 mm at 4062 meters, and 75 mm at 1518 meters. The cost of planning a clean water distribution system in 2029 is Rp. 1,431,375,000.00.

Distribusi air bersih di Kecamatan Genteng Kabupaten Banyuwangi merupakan wilayah pelayanan Zona 1 PDAM unit Genteng. Kapasitas debit reservoir sebesar 60 liter/detik berasal dari sumber umbul sari di Kecamatan Glenmore. Pendistribusian air bersih di wilayah Zona 1 masih kurang dari 70% wilayah yang terlayani, karena diperkirakan kapasitas debit yang terpasang kurang mencukupi. Untuk memenuhi target pemerataan distribusi perlu pengembangan sistem jaringan dengan penambahan debit dan perencanaan jaringan pipa baru. Program Epanet 2.0 memudahkan dalam perhitungan jaringan perpipaan dengan mengintegrasi data elevasi, peta jaringan, spesifikasi pipa dan debit. Analisis hasil simulasi menggunakan standar parameter hidrolis Permen PU 2007. Perencanaan pengembangan jaringan distribusi dan anggaran biaya pada tahun 2029 guna memperkirakan debit kebutuhan air dan anggaran biaya yang dibutuhkan. Hasil simulasi hidrolis berdasarkan analisis tekanan semua junction telah sesuai standar, sedangkan analisis kecepatan masih di bawah standar. Kebutuhan debit air tahun 2029 sebesar 71,6 Liter/detik. Pengembangan pipa distribusi direncanakan di Desa Kembiritan dengan tambahan satu unit reservoir. Dimensi pipa rencana di wilayah pengembangan digunakan diameter 25 mm sepanjang 796 m, diameter 50 mm sepanjang 4062 m dan diameter 75 mm sepanjang 1518 m. Biaya perencanaan sistem distribusi air bersih tahun 2029 sebesar Rp. 1.431.375.000,00.
Production of pomegranate snacks as affected by different pre-treatments
Authors: ERSUS Seda , ÇETİNER Miray , HAYATİOĞLU Nergiz , HEPÇİMEN A. Zeki
Published: May 2020

Objective: In this research, the production and quality characteristics of new healthy pomegranate snacks (Punica granatum L.) obtained by drying after different pre-treatments were investigated.
Material and Methods: Besides control group, three different pre-treatments were applied to the pomegranate seeds before drying. Pomegranate seeds without any pre-treatment were grouped as “control” samples (C) and other group was boiled in 80 °C water for 2 minutes “boiled” (B). Samples treated with sucrose and glucose (60 °Brix) solutions (18 hours at 40 °C) for osmotic dehydration were grouped as "sucrose” (S) and “glucose" (G) group. Samples in all groups were dried at 65 °C in a tray dryer at an air velocity of 1.6 m/s until the moisture content reached 6%.
Results: According to physical and chemical analysis results, the total sugar content was between 87.96-97.50 g/100 g; the amount of invert sugar 46.23-62.75 g/100 g; the total amount of phenolic substance 255.6-407.6 mg/100 g; antioxidant capacity values ranged from 40.2% to 49.6%. Total loss of phenolic substance according to raw material was found to be the lowest in boiled samples. According to sensory tests in terms of all sensory properties (color, texture, flavour and preference), boiling pre-treatment applied sample was determined as the most preferred sample.
Conclusion: When all results are analysed, to obtain a healthy snack product using pomegranate fruit, it is thought that applying the boiling pre-treatment before drying to the pomegranate may be appropriate for preserving nutrients and obtaining a preferred snack pomegranate.
Avant-propos
Authors: POULIQUEN H.
Published: May 2020

L’INRA et l’IFREMER ont chargé les responsables de la coordination entre les deux organismes de l’organisation d’un colloque consacré à la santé des espèces aquacoles.Les objectifs de ce colloque ont été de dresser un bilan des compétences et des connaissances, d'évaluer les forces et faiblesses du dispositif français de recherche et d'identifier des thèmes prioritaires sur lesquels la mise en commun des compétences permettrait d'optimiser le dispositif.
Les thèmes abordés lors de ce colloque n’ont pas fait l’objet, lors de sa tenue en juin 2006, d’une production écrite spécifique. Ce numéro spécial d’INRA Productions Animales constitue donc un ouvrage de référence original dans la littérature scientifique en santé des espèces aquacoles, notamment parce que les synthèses ont été complétées d’articles plus spécifiques dans le domaine de la santé des espèces piscicoles ou conchylicoles.Ce numéro spécial est divisé en trois parties :– outils de diagnostic et émergence des maladies infectieuses aquacoles, – épidémiologie et modélisation des maladies infectieuses aquacoles, – mesures de gestion du risque d’apparition de maladies infectieuses aquacoles.Je tiens à exprimer ma reconnaissance aux auteurs et aux relecteurs pour avoir accepté ce surcroît de travail nécessaire à une plus large diffusion d’informations récentes et originales en matière de santé des espèces aquacoles.
Facteurs de risque d’apparition et d’émergence des maladies infectieuses en aquaculture
Authors: RENAULT T. , GUICHARD B.
Published: May 2020

Au cours des dernières années, l’augmentation de la production aquacole et du nombre d’espèces aquatiques élevées au niveau mondial s’est accompagnée d’une augmentation du nombre et de la répartition des maladies infectieuses. En 2002-2003, 62 cas d’émergence de ma-ladies infectieuses chez des animaux aquatiques ont été rapportés, les deux tiers en élevage, principalement en Europe et en Amérique du Nord. Une maladie est dite émergente quand son incidence augmente significativement pour une population, un lieu et une période donnés. Pour émerger, une maladie doit d’abord être introduite dans une zone ou une population indemne, et les animaux être exposés à des facteurs favorisants sa transmission. Les risques d’introduction de nouvelles maladies sont principalement associés aux mouvements d’animaux infectés (sauvage ou d’élevage, vivants ou morts), au matériel d’élevage ou à des milieux de transport contaminés, à l’évolution des agents pathogènes eux-mêmes, et à des facteurs environnementaux : réservoirs sauvages, polluants, changements climatiques. Les facteurs d’exposition permettant l’émergence d’une maladie après son introduction sont liés aux conditions d’élevage, à l’épidémiologie des différentes maladies et aux mouvements d’eau et d’animaux sauvages.
COVID-19 Management in Spain. Errors to Avoid and the Need of A Post-Confinement Longitudinal Epidemiological Study
Authors: Franco Rafael
Published: May 2020

Some of the European countries affected by COVID-19 have not learned from previous experience in China. Italy did not learn from China and Spain did neither learn from China nor from Italy. Confinement in Spain was postponed due to pressure from economic interests and traveling of infected people, especially from Madrid to the beaches in South-East Spain, was allowed. Strict confinement and border closure came late, when the curve of infected people and the death toll already had the worse trend worldwide. Tests to SARS-CoV-2 RNA detection by PCR, were first unavailable and, later, faulty and/or detecting antibodies and not the virus itself. Instead of mobilizing research laboratories for making tests, and instead of making masks and ventilators, mediatic scientists asked for money for controversial clinical trials and for obtaining a vaccine. In this scenario, common sense indicates that ad hoc measures should be taken at the end of confinement in order to minimize pain. The chain of errors should be avoided in the management of next pandemics by designing Good Practice Rules (GPRs). In addition, post-confinement measures should be implemented as soon as possible to be ready for SARS-CoV-2 return next season. In this sense a longitudinal study in the most affected cities (Madrid, Barcelona, Vitoria and Pamplona) should be performed with the primary objective of detecting carriers with no symptoms, to stratify patients according to symptoms, and to early detection of virus reappearance.
Covid-19 and Other Viruses in Brazil: Can the New Pandemic Influence Epidemiological Records?
Authors: Fernandes Haniel
Published: May 2020

Amid the covid-19 pandemic, other diseases, including viruses, are still acting to the detriment of their seasonality and risk factors for contagion. For this reason, it is interesting to know the degree of impact of other viruses, mainly respiratory, in which they have similar symptoms, in diagnoses for contamination by the new coronavirus based on epidemiological surveys, via epidemiological weeks, in Brazil. To what extent there may be a hypothesis of confusion of contaminated data, harming the health system, with regard to the need for intensive care units and control of viruses, and negatively or positively implying in the control or uncontrolling of viruses in general.
Plasmids Shape the Diverse Accessory Resistomes of Escherichia coli ST131
Authors: Decano Arun G. , Tran Nghia , Al-Foori Hawriya , Al-Awadi Buthaina , Campbell Leigh , Ellison Kevin , Mirabueno Louisse P. , Nelson Maddy , Power Shane , Smith Genevieve , Smyth Cian , Vance Zoe , Woods Caitriona , Rahm Alexander , Downing Tim
Published: May 2020

The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. Escherichia coli is a significant component of the gastrointestinal microbiome and is typically non-pathogenic in this niche. In contrast, extra-intestinal pathogenic E. coli (ExPEC) including ST131 may occupy other environments like the urinary tract or bloodstream where they express genes enabling AMR and host adhesion like type 1 fimbriae. The extent to which non-pathogenic gut E. coli and infectious ST131 share AMR genes and key associated plasmids remains understudied at a genomic level. Here, we examined AMR gene sharing between gut E. coli and ST131 to discover an extensive shared preterm infant resistome. In addition, individual ST131 show extensive AMR gene diversity highlighting that analyses restricted to the core genome may be limiting and could miss AMR gene transfer patterns. We show that pEK499-like segments are ancestral to most ST131 Clade C isolates, contrasting with a minority with substantial pEK204-like regions encoding a type IV fimbriae operon. Moreover, ST131 possess extensive diversity at genes encoding type 1, type IV, P and F17-like fimbriae, particular within subclade C2. The type, structure and composition of AMR genes, plasmids and fimbriae varies widely in ST131 and this may mediate pathogenicity and infection outcomes.
Study on Machine Learning and Deep Learning Methods for Human Action Recognition
Authors: Rajendran Gopika , Thomas Lee Ojus , Gopi Arya , jose Jais , Gautham Neha
Published: May 2020

With the evolution of computing technology in many application like human robot interaction, human computer interaction and health-care system, 3D human body models and their dynamic motions has gained popularity. Human performance accompanies human body shapes and their relative motions. Research on human activity recognition is structured around how the complex movement of a human body is identified and analyzed. Vision based action recognition from video is such kind of tasks where actions are inferred by observing the complete set of action sequence performed by human. Many techniques have been revised over the recent decades in order to develop a robust as well as effective framework for action recognition. In this survey, we summarize recent advances in human action recognition, namely the machine learning approach, deep learning approach and evaluation of these approaches.
Effect of COVID-19 on the Indian Economy and Supply Chain
Authors: Agrawal Shruti , Jamwal Anbesh , Gupta Sumit
Published: May 2020

At present time world is facing from the coronavirus disease known as Covid-19. The first case of the coronavirus was reported in the December, 2019 in the Wuhan city of China which is known as the major transportation hub of China. After the spread of Covid-19 many countries have shut down their sea ports and airports. They have banned the import and export activities. Also, China is the major distributor of the raw materials which affect the manufacturing activities across the globe due to lockdowns. India is the developing country due to the Covid-19 spread the cases reported in the India government has lockdown the country for 41 days which affected the manufacturing activities and majorly it affects the supply chains and economy of the country. In the present paper we have discussed the effect of Covid-19 on Indian economy and on supply chains in India. There are total of 18 critical barriers are found out which affected the supply chains in the India. It is expected that this study will helpful the researchers to develop the conceptual models to overcome from this issue.
Flattening COVID-19 Curve in Egypt: An Epidemiological Modelling
Authors: Hasab Ali
Published: May 2020

Background: Flattening the curve refers to community isolation measures that keep the daily number of disease cases at a manageable level for medical providers. Efforts to completely contain the COVID – 19 have failed. As there is currently no vaccine or specific medication to treat COVID-19, the only way to flatten the curve is through collective action of mitigation efforts. Objectives: The study was carried out with aim to study the effect of mitigation efforts in reducing the overall COVID -19 attack rate and flattening the curve of COVID-19 in Egypt. Methods: Secondary data in the situation reports of WHO, Worldometer and Egyptian MoHP Report about the COVID-19 epidemic in Egypt were analysed till April 24, 2020. The Susceptible Infected Recovered (SIR) model was used to study the epidemic dynamics of COVID-19 pandemic. Results: In Egypt in fourth week after exposure β was 0.27 and γ = 0.06. The fraction of infectious individuals will grow exponentially as the exponential growth rate was 0.21, with an epidemic time of 4 days. R in 23 rd April it was 0.18 (less than one person infected) where β=0.07 and γ =0.39. There was reduction in the actual overall attack rate up to 23th April of 70 % of its value without intervention. There was flattening of the curve. Conclusion and Recommendation: Mitigation efforts of Egypt had succeeded in flattening the curve of COVID-19 Pandemic. Even though, strengthening all the mitigation efforts to keep the exposure probability, as well as the transmission rate as low as possible is mandatory for containment of the epidemic.
3d Printing Technology and Internet of Things Prototyping in Family Practice: Building Pulsoximeters during COVID-19 Pandemic
Authors: Capobussi Matteo , Moja Lorenzo
Published: May 2020

Family doctors can have an active role in identifying significant population needs and solutions. During COVID-19 epidemic, patient home monitoring with pulse oximetry has been a key aspect of care of patients. However, pandemics bring shortage medical equipment such as pulse oximetry. Through the local maker community in a matter of days four “smart” pulsoximeters were created and built. Following Internet of Things principles, the pulsoximeters were programmed to transmit recorded data through Wi-Fi, in real time, directly to the doctors. Each protype pulsoximeter served a family doctor during the pandemic. Building instructions were shared in maker-oriented websites, potentially leading to additional small-scale productions.
Ribosomal Proteins Homologies and Methylation of Mitoribosome by Corona Virus Nsp9, Nsp10 and Previously Described Nsp13-16 Proteins Suggested Their Roles in the Inhibition of Host Protein Synthesis: Discovery of New Therapeutic Targets against Corona Virus Infections
Authors: Chakraborty Asit Kumar
Published: May 2020

Multi-Alignment method coupled with phylogenetic analysis we disclosed the Nsp9 and Nsp10 non-structural proteins of Corona Virus as rRNA RlmH/K methyltransferases with similarities with bin recombinase and int-core integrase fold. Further, Nsp9 has similarities to S8 ribosomal protein and Nap10 has similarity to S10 ribosomal protein. Previously, we showed Nsp13, Nsp14, Nsp15 and Nsp16 are also different types of rRNA RlmE/N and Cfr-like methyltransferases-ribonuclease with RNA helicase domains. Two domains of Nsp13 astonishingly have similarities to ribosomal proteins L6 and L9. Taken together, Nsp9/10 and Nsp13-16 proteins could mimic host ribosome assembly and also could methylate rRNA of mitobibosome preventing mitochondrial protein synthesis and oxidative phosphorylation. Low ATP synthesis causes lowering blood pressure following coma but very ATP concentration (1-10nM) surely induces platelets aggregation through vWA, collagen and GpIIb/IIIa proteins followed by fibrin formation and blood clotting as recently have seen in the lung of many Corona virus infected patients. We have also postulated that two polyproteins itself resemble like 28S and 38S mitoribosome subunits and compete with rRNAs inhibiting the ribosome turnover and new protein synthesis due to their similarities with many ribosomal proteins. Such finding may be valuable in computer-based novel drug design against Corona virus.
Deteriorating Atrioventricular Block in COVID-19 Suspected Patient after Receiving Initial Dose of Azythromycin and Hydroxychloroquine
Authors: Pratama Billy Aditya , Rachman Afik Maulana , Anggraeni Vita Yanti , Maharani Erika , Trisnawati Ika , Riyanto Bambang Sigit , Hartopo Anggoro Budi
Published: May 2020

Coronavirus 2019 (COVID-19) is an infectious disease that is becoming a pandemic. Hydroxychloroquine in combination with azythromycin are among drugs currently in use to eradicate COVID-19. Despite concerns due to its potential cardiac toxicity, hydroxychloroquine is widely accepted in mild and moderate COVID-19 pneumonia. In this case report, we report a case of a young Indonesian adult male with suspected COVID-19 pneumonia who received hydroxychloroquine and azythromycin therapies and during 24 hour experienced deterioration of atrioventricular block.
O Processo Orçamentário de uma Instituição Pública Federal de Ensino sob a Ótica do Isomorfismo
Authors: Zambenedetti Lidiane , Angonese Rodrigo
Published: May 2020

Esta pesquisa tem como objetivo compreender como o isomorfismo explica o processo orçamentário de uma instituição pública federal de ensino, conforme o modelo de Dimaggio e Powell (1983). A pesquisa, de caráter qualitativo e exploratória, adota a perspectiva da Teoria Institucional. A coleta dos dados ocorreu por meio de  entrevistas, análise documental e observação não participante. Os resultados mostram que o processo orçamentário da instituição é influenciado pelos três tipos de isomorfismo (coercivo; mimético; normativo), em conformidade com a nova sociologia institucional. Observou-se que os três tipos de isomorfismo atuam simultaneamente, influenciando o processo orçamentário, com destaque para o isomorfismo coercivo. O isomorfismo normativo é o menos explorado.
Accelerometry Assessed Physical Activity of Older Adults Hospitalized with Acute Medical Illness - an Observational Study
Authors: Tasheva Plamena , Kraege Vanessa , Vollenweider Peter , Roulet Guillaume , Méan Marie , Marques-Vidal Pedro
Published: May 2020

Background: In a hospital setting and among older patients, inactivity and bedrest are associated with a wide range of negative outcomes such as functional decline, increased risk of falls, longer hospitalization and institutionalization. Our aim was to assess the distribution, determinants and predictors of physical activity (PA) levels using wrist-worn accelerometers in older patients hospitalized with acute medical illness.Methods: Observational study conducted from February to November 2018 at an acute internal medicine unit in the University hospital of Lausanne, Switzerland. We enrolled 177 patients aged ≥65 years, able to walk prior to admission. PA during acute hospital stay was continuously recorded via a 3D wrist accelerometer. Clinical data was collected from medical records or by interview. Autonomy level prior to inclusion was assessed using Barthel Index score. PA levels were defined as <30 mg for inactivity, 30-99 mg for light and ≥100 for moderate PA. Physically active patients were defined as 1) being in the highest quartile of time spent in light and moderate PA or 2) spending ≥20 minutes/day in moderate PA.Results: Median [interquartile range - IQR] age was 83 [74-87] years and 60% of participants were male. The median [IQR] time spent inactive and in light PA was 613 [518-663] and 63 [30-97] minutes/day, respectively. PA peaked between 8 and 10 am, at 12 am and at 6 pm. Less than 10% of patients were considered physically active according to definition 2. For both definitions, active patients had a lower prevalence of walking aids and a lower dependency level according to Barthel Index score. For definition 1, use of medical equipment was associated with a 70% reduction in the likelihood of being active: odds ratio (OR) 0.30 [0.10-0.92] p=0.034; for definition 2, use of walking aids was associated with a 75% reduction in the likelihood of being active: OR=0.24 [0.06-0.89], p=0.032.Conclusion: Older hospitalized patients are physically active only 10% of daily time and concentrate their PA around eating periods. Whether a Barthel Index below 95 prior to admission may be used to identify patients at risk of inactivity during hospital stay remains to be proven.
Early prediction of pathologic response to neoadjuvant treatment of breast cancer: use of a cell-loss metric based on serum thymidine kinase 1 and tumour volume
Authors: Tribukait Bernhard
Published: May 2020

Background: After neoadjuvant chemotherapy of breast cancer pathologic complete response (pCR) indicates a favorable prognosis. Among non-selected patients, pCR is, however, achieved in only 10-30%. Early evaluation of tumour response to treatment would facilitate individualized therapy, with ineffective chemotherapy interrupted or changed. The methodology for this purpose is still limited. Tumour imaging and analysis of macromolecules, released from disrupted tumour cells, are principal alternatives.Objective: To investigate whether a metric of cell-loss, defined as the ratio between serum concentration of thymidine kinase1 (sTK1, ng x ml-1) and tumour volume, can be used for early prediction of pathologic response.Methods: 104 women with localized breast cancer received neoadjuvant epirubicin/docetaxel in 6 cycles, supplemented with bevacizumab in cycles 3-6. The cell-loss metric was established at baseline (n=104), 48h after cycle 2 (n=104) and prior to cycle 2 (n=57). The performance of the metric was evaluated by association with pathologic tumour response at surgery 4 months later.Results: Treatment caused a rise in sTK1, a reduction in tumour volume and a marked increase in the cell-loss metric. Patients were subdivided into quartiles according to the baseline cell-loss metric. For these groups, baseline means were 0.0016, 0.0042, 0.0062, 0.0178 units. After subtraction of baselines, means for the quartiles 48h after treatment 2 were 0.002, 0.011, 0.030 and 0.357 units. pCR was achieved in 24/104, their distribution in the quartiles (11%, 11%, 23% and 46%) differed significantly (p=0.01). In 80 patients with remaining tumour, tumour size was inversely related to the metric (p=0.002). In 57 patients studied before treatment 2, positive and negative predictive values of the metric were 77.8% and 83.3%, compared to 40.5% and 88.7% 48h after treatment 2.Conclusion: A cell-loss metric, based on serum levels of TK1, released from disrupted tumour cells, and tumour volume, reveal tumour response early during neoadjuvant treatment. The metric reflect tumour properties that differ greatly between patients and determine the sensitivity to cytotoxic treatment. The findings point to the significance of cell loss for tumour growth rate. The metric should be considered in personalized oncology and in the evaluation of new therapeutic modalities.Trial registration: PROMIX (Clinical Trials.gov NCT000957125).https://clinicaltrials.gov/ct2/show/NCT00957125
Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro
Authors: Xing Hang , Xu Sihan , Xie Xin'e , Wang Yuan , Lu Cheng Biao , Han Xiong
Published: May 2020

The authors have withdrawn this preprint due to author disagreement.
Assessment of Knowledge, Attitude and Practices and the Analysis of Risk Factors regarding Schistosomiasis Among Fishermen and Boatmen in the Dongting Lake Basin, P.R. China
Authors: Guan Zhou , Dai Si-Min , Zhou Jie , Ren Xiao-Bing , Qin Zhi-Qiang , Li Yin-Long , Lv Shan , Li Shi-Zhu , Zhou Xiao-Nong , Xu Jing
Published: May 2020

Background: Fishermen and boatmen are a risk population for contracting schistosomiasis due to their high frequency of water contact in endemic areas of schistosomiasis in the People’s Republic of China (P.R. China). To develop specific interventions towards this population, our present study was designed to assess the knowledge, attitudes and practices (KAPs) towards schistosomiasis of fishermen and boatmen, and to identify the risk factors associated with schistosome infection using molecular technique in the selected area of Hunan Province in P.R. China. Methods: A cross sectional survey was conducted in the Dongting Lake Basin of Yueyang County, Hunan Province, P.R. China. A total of 601 fishermen and boatmen were interviewed between October and November 2017. Information regarding socio-demographic details and KAPs towards schistosomiasis were collected using a standardized questionnaire. Fecal samples of participants were collected and tested by Polymerase Chain Reaction (PCR). Logistic regression analysis was conducted to explore the risk factors related to the positive results of PCR. Results: Of the 601 respondents, over 90% knew schistosomiasis and how the disease was contracted, the intermediate host of schistosomes and preventive methods. The majority of respondents had a positive attitude towards schistosomiasis prevention. However, only 6.66% (40/601) of respondents had installed a latrine on their boats, while 32.61% (196/601) of respondents defecated in the public toilets on shore. In addition, only 4.99% (30/601) respondents protected themselves while exposed to freshwater. The prevalence of schistosomiasis, as determined by PCR, among fishermen and boatmen in Yueyang County was 13.81% (83/601). Age, years of performing the current job, number of times receiving treatment, and whether they were treated in past three years were the main influencing factors of PCR results among this population. Conclusions: Fishermen and boatmen are still at high risk of infection in P.R. China and gaps exist in KAPs towards schistosomiasis in this population group. Chemotherapy, and health education encouraging behavior change in combination with other integrated approaches to decrease the transmission risk in environments should be improved.
Genome-wide identification and characterization of lectin receptor-like kinase gene family in cucumber and expression profiling analysis under different treatments
Authors: Lv Duo , Wang Gang , Xiong Liang-Rong , Sun Jing-Xian , Chen Yue , Guo Chun-Li , Yu Yao , He Huan-Le , Cai Run , Pan Junsong
Published: May 2020

Background Lectin receptor-like kinases (LecRLKs) are a class of membrane proteins found in plants that are involved in diverse functions, including plant development and stress responses. Although LecRLK families have been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in cucumber ( Cucumis sativus L.). Results In this study, 46 putative LecRLK genes were identified in cucumber genome, including 23 G-type, 22 L-type and 1 C-type LecRLK genes. They unequally distributed on all 7 chromosomes with a clustering trendency. Most of the genes in the cucumber LecRLK ( Cs ecRLK ) gene family lacked introns. In addition, there were many regulatory elements associated with phytohormone and stress on these genes’ promoters. Transcriptome data demonstrated that distinct expression patterns of CsLecRLK genes in various tissues. Furthermore, we found that each member of the CsLecRLK family had its own unique expression pattern under hormone and stress treatment by the quantitative real time PCR (qRT-PCR) analysis. Conclusion This study provides a better understanding of the evolution and function of LecRLK gene family in cucumber, and opens the possibility to explore the roles that LecRLK s might play in the life cycle of cucumber.
Effect of Porphyromonas gingivalis infection on gut dysbiosis and arthritis exacerbation in mouse model
Authors: Hamamoto Yuta , Ouhara Kazuhisa , Munenaga Syuichi , Shoji Mikio , Ozawa Tatsuhiko , Hisatsune Jyunzo , Kado Isamu , Kajiya Mikihito , Matsuda Shinji , Kawai Toshihisa , Mizuno Noriyoshi , Fujita Tsuyoshi , Hirata Shintaro , Tanimoto Kotaro , Nakayama Koji , Kishi Hiroyuki , Sugiyama Eiji , Kurihara Hidemi
Published: May 2020

Background: Porphyromonas gingivalis (Pg) infection causes periodontal disease and is involved in the exacerbation of rheumatoid arthritis (RA). Gut microbiota dysbiosis has shown strong associations with systemic diseases, including RA, diabetes mellitus, and inflammatory bowel disease, and inoculation of periodontopathogenic bacteria (i.e. Pg) can alter gut microbiota composition. Therefore, this study investigated dysbiosis-mediated arthritis exacerbation by Pg oral inoculation in an experimental arthritis model mouse.Methods: Pg inoculation in the oral cavity twice a week for 6 weeks was performed to induce periodontitis in SKG mice. Concomitantly, a single intraperitoneal (i.p.) injection of laminarin (LA) was administered to induce experimental arthritis (Pg-LA mouse). Citrullinated protein (CP) and IL-6 levels in periodontal, intestinal, and joint tissues, and serum were measured by ELISA. Gut microbiota composition was determined by sequencing the bacterial 16S rRNA after DNA purification of mouse feces. Fecal microbiota transplantation (FMT) was performed by transferring Pg-RA-derived feces to normal SKG mice. The effect of the Pg peptidylarginine deaminase (PgPAD) on the level of citrullinated proteins and arthritis progression were determined using PgPAD knockout mutant.Results: Periodontal alveolar bone loss and IL-6 in gingival tissue were induced by Pg oral infection, as well as severe joint destruction, increased arthritis scores (AS), and IL-6 and CP production in serum, joint, and intestinal tissues. Distribution of Deferribacteres and S24-7 was decreased, while CP was significantly increased in gingival, joint, and intestinal tissues of Pg-inoculated experimental arthritis mice compared to experimental arthritis mice without Pg inoculation. Further, FMT from Pg-inoculated experimental arthritis mice showed the reproduction of donor gut microbiota and resulted in severe joint destruction with increased IL-6 and CP production in joint and intestinal tissues. The avarage AS of FMT from Pg-inoculated experimental arthritis was much higher than that of donor mouse. However, inoculation of the PgPAD knockout mutant inhibited the elevation of arthritis scores, ACPA level in serum and reduced CP amount in gingival, joint, and intestinal tissues compared to Pg wild type inoculation .Conclusion: Pg oral infection affected gut microbiota dysbiosis and joint destruction via increased CP generation.
Two-by-two factorial randomised study within a trial (SWAT) to evaluate strategies for follow-up in a randomised prevention trial
Authors: Bradshaw Lucy , Montgomery Alan A , Williams Hywel , Chalmers Joanne , Haines Rachel
Published: May 2020

Background Failure to collect outcome data in randomised trials can result in bias and loss of statistical power. Further evaluations of strategies to increase retention are required. We assessed the effectiveness of two strategies for retention in a randomised prevention trial using a two-by-two factorial randomised study within a trial (SWAT). Methods Parents of babies included in the host trial were randomised to (1) Short message service (SMS) notification prior to sending questionnaires at 3, 6, 12 and 18 months versus no SMS notification and (2) £10 voucher sent with the invitation letter for the primary follow up visit at 24 months or given at the visit. The two co-primary outcomes were collection of host trial (1) questionnaire data at interim follow-up times and (2) primary outcome at 24 months during a home/clinic visit with a research nurse. Results Between November 2014 and November 2016, 1394 participants were randomised: 350 to no SMS + voucher at visit, 345 to SMS + voucher at visit, 352 to no SMS + voucher before visit and 347 to SMS + voucher before visit. Overall questionnaire data was collected at interim follow-up times for 75% in both the group allocated to the prior SMS notification and the group allocated to no SMS notification (odds ratio (OR) SMS v. none 1.02, 95% CI 0.83 to 1.25). Host trial primary outcome data was collected at a visit for 557 (80%) allocated to the voucher before the visit in the invitation letter and for 566 (81%) whose parents were allocated to receive the voucher at the visit (OR before v. at visit 0.89, 95% CI 0.69 to 1.17). Conclusion There was no evidence of a difference in retention according to SMS notification or voucher timing. Future synthesis of SWAT results is required to be able to detect small but important incremental effects of retention strategies. Trial registration: Host trial registration: ISRCTN registry; ID: ISRCTN21528841. Registered on 25 July 2014. SWAT Repository Store ID 25
DL-3-n-butylphthalide improves cerebral hypoperfusion in patients with large cerebral atherosclerotic stenosis. A single-center, randomized, double-blind, placebo-controlled study.
Authors: Chen Dawei , Yin Yanwei , Shi Jin , Yang Fen , Wang Kehua , Zhao Faguo , Li Wenping , Li Bin
Published: May 2020

Background: DL-3-n-butylphthalide (NBP) was demonstrated to increase the cerebral blood flow (CBF) in the animal models, but there are no clinic studies to verify this. We aimed to explore the effect of NBP on improving cerebral hypoperfusion caused by cerebral large-vessel stenosis.Methods: In this single-center, randomized, double-blind, placebo-controlled study, 120 patients with severe carotid atherosclerotic stenosis and cerebral hypoperfusion in the ipsilateral middle cerebral artery (MCA) were included and randomly assigned into NBP or placebo group as 1:1 radio. Patients in NBP or placebo group received 200mg or 20mg of NBP capsules three times daily for four weeks respectively. Single photon emission computed tomography (SPECT) was used to assess regional CBF (rCBF) in four regions of interest (ROIs) corresponding to MCA before and 12 weeks after the treatment. After therapy, the rCBF change for every ROI and the whole CBF change in MCA territory for every patient were classified into amelioration, stabilization and deterioration respectively.Results: 48 NBP patients (6 with bilateral stenosis) and 46 placebo patients (8 with bilateral stenosis) completed the trial. Overall, both groups had 54 stenotic carotid arteries and 216 ROIs for rCBF change analysis. After therapy, the rCBF in ROIs increased in NBP group (83.5%±11.4% vs. 85.8%±12.5%, p=0.000), whereas no change was found in placebo group (86.9%±11.6% vs. 87.8%±11.7%, p=0.331). Besides, there was higher percentages of ROIs with rCBF amelioration and stabilization in NBP group than in placebo group (93.1% vs. 79.2%, p=0.000). Furthermore, ordinal regression analysis showed that compared with placebo, NBP independently made more patients to have whole CBF amelioration in ipsilateral MCA (Wald-χ2=5.247, OR=3.31, p=0.022).Conclusions: NBP might improve the cerebral hypoperfusion in the patients with carotid artery atherosclerotic stenosis.Trial registration: Chinese Clinical Trial Registry, ChiCTR1900028005, registered December 8th 2019- Retrospectively registered (http://www.chictr.org.cn/index.aspx).
HiCoP, a simple and robust method for detecting interactions of regulatory regions
Authors: Zhang Yan , Li Zhaoqiang , Bian Shasha , Zhao Hao , Feng Delong , Chen Yanhong , Hou Yuhe , Liu Qifa , Hao Bingtao
Published: May 2020

Background: Chromatin physical interactions provide essential information for understanding the regulation of cis-elements like enhancers, promoters, and insulators in cell development and differentiation. The Hi-C assay is a technique detecting chromatin structures of the whole genome but not sensitive to interactions of regulatory elements. Several methods, like HiChIP, DNase-C, and OCEAN-C, have been developed for enriching interactions of regulatory regions, but all of them have some shortcomings. New simple, efficient, and robust methods are still in need of detecting interactions of regulatory regions. Results: We developed a new, simple, and robust assay called CoP (Column Purified chromatin) for profiling of open chromatin regions by directly purifying fragmentized crosslinked chromatin with a DNA purification column. The accessible chromatin regions, including active enhancers, promoters, and insulators, were significantly enriched in CoP chromatin. The CoP-seq assay can efficiently detect open chromatin regions, especially active promoters, with a high signal-to-noise ratio. We integrated the CoP-seq and Hi-C technique (HiCoP) for the detection of interactions of accessible chromatin regions, which represent active cis-regulatory elements in cells. We observed that the HiCoP captured the peaks in the promoters-associated enhancer regions, and the chromatin features identified by HiCoP were similar to HiChIP of histone H3K27 acetylation rather than Hi-C. HiCoP detected more promoter-enhancer (P-E), promoter-promoter (P-P), and enhancer-enhancer (E-E) interactions within 20kb-5Mb than Hi-C. Most of the loops identified by HiCoP were associated with the expressed genes. Conclusion: CoP assay can efficiently enrich open chromatin regions. When CoP assay was integrated with Hi-C assay, it provides a simple, robust, alternative technique for profiling accessible chromatin regions and chromatin conformation simultaneously.