bluuebunny/crossref_metadata_2025 huggingface dataset:
Zinc oxide nanoparticles improve lactation and metabolism in dairy goats by modulating the rumen microbiota
Authors: Xie Shan , Ying Zaixiang , Xiu Ziqing , Sun Yawang , Yang Qinlin , Gao Hanyu , Fan Wenqiao , Wu Yongjiang
Published: November 2024

This study aimed to investigate the effects of dietary supplementation with zinc oxide nanoparticles (ZnONPs) on lactation, rumen microbiota, and metabolomics in dairy goats. Twenty Guanzhong dairy goats, with comparable milk yields and in the mid-lactation stage, were randomly divided into two groups, with 10 goats in each group. The control group was fed a standard diet, while the ZnONP group received the control diet plus 30 mg ZnONPs/kg DM. The pre-trial period lasted for 7 days, followed by a trial period of 30 days. The results showed that the addition of ZnONPs increased the milk yield and milk fat content (p < 0.05). The results of rumen microbial sequencing showed that the Chao1, Observed species, and PD_whole_tree indices of the ZnONP group were higher than those of the control group. The addition of ZnONPs altered the composition of the rumen microbiota, increasing the abundance of beneficial bacteria (Prevotella and Rikenellaceae_RC9_gut_group) and decreasing the abundance of the harmful bacterium Sediminispirochaeta. Non-targeted metabolomics analysis identified a total of 261 differential metabolites between the two groups, indicating changes in rumen metabolism. Further correlation analysis revealed a positive correlation between beneficial bacteria (Rikenellaceae RC9 gut group and Anaeroplasma) and metabolites such as nicotinamide riboside, inosine, and guanosine (p < 0.05). In addition, a positive correlation was observed between milk yield and beneficial bacteria (RF39 and Clostridia vadinBB60 group), as well as between milk fat content and Quinella (p < 0.05). In summary, ZnONP supplementation can improve the structure of the rumen microbiota in dairy goats, positively influencing milk yield, milk composition, and metabolism.
The clinical value of fibrosis indices for predicting the hemorrhagic transformation in patients with acute ischemic stroke after intravenous thrombolysis
Authors: Chen Jiahao , Li Xiaoqin , Hu Rui , Hu Chuanchen
Published: November 2024

Background

The incidence of stroke in China is approximately 343 per 100,000 people each year, the highest rate worldwide. Hemorrhagic transformation (HT), particularly symptomatic intracerebral hemorrhage (sICH) following acute ischemic stroke (AIS) with or without intravenous thrombolysis (IVT), can lead to rapid neurological deterioration, poor prognosis, and even death. Non-alcoholic fatty liver disease (NAFLD) has been identified as a risk factor for stroke occurrence and associated with poor long-term functional outcomes. Nonetheless, no studies have examined the association between liver fibrosis and HT in AIS patients who underwent IVT.

Method

A total of 826 patients with AIS who underwent IVT were included in this study. We calculated nine validated liver fibrosis indices to assess the extent of liver fibrosis. HT was detected by follow-up cranial CT/MRI within 24 h post-IVT and was classified as either hemorrhagic infarction (HI) or parenchymal hematoma (PH). Symptomatic intracranial hemorrhage was defined as a sudden symptomatic neurological deterioration, indicated by an increase in (National Institutes of Health Stroke Scale) NIHSS score of 4 points or more.

Result

The median values of fibrosis-4 (FIB-4), modified FIB-4 (mFIB-4), aspartate aminotransferase (AST)-platelet ratio index (APRI), Forns index, alanine aminotransferase (ALT)/AST (ARR), AST/ALT ratio-platelet ratio index (AARPRI), fibrosis quotient (FibroQ), and Fibrosis Index were significantly higher, while the fibrosis-5 (FIB-5) was significantly lower in the HT and sICH groups (all P < 0.001). After adjusting for potential confounders, all nine liver fibrosis indices remained associated with HT and sICH. Receiver operating characteristic (ROC) curve analysis revealed that the FibroQ score had the best predictive ability for HT (AUC = 0.707, CI = 0.652–0.762, P < 0.001), while FIB-4 had the best predictive ability for sICH (AUC = 0.802, CI = 0.711–0.892, P < 0.001).

Conclusion

Liver fibrosis, as validated by FIB-4, mFIB-4, FIB-5, APRI, Forns index, ARR, AARPRI, FibroQ, and Fibrosis Index, was associated with HT and sICH in AIS patients after IVT.
Interlaminar and varicose-projection astrocytes: toward a new understanding of the primate brain
Authors: Ciani Caterina , Falcone Carmen
Published: November 2024

In the last years, science started to move toward a more glio-neurocentric view, in which astrocytes are hypothesized to be directly involved in cognitive functions. Indeed, astrocytes show a variety of shapes with species-specific characteristics, suggesting a specialization of roles during evolution. Interlaminar (ILA) and varicose-projection (VP-As) astrocytes show an anatomical organization that is different compared to the classical horizontal net typically formed by protoplasmic and fibrous astrocytes. ILAs show a modular architecture with the soma in the first cortical layer and processes toward the deep layers with species-specific length. VP-As reside in the deep layers of the cortex, are characterized by varicosities on the longest processes, and are individual-specific. These characteristics suggest roles that are more complex than what was theorized until now. Here, we recapitulate what we know so far from literature from the first time ILAs were described to the most recent discoveries, spanning from morphology description, hypothesis on the development to their features in diseases. For a complete glance on this topic, we included a final paragraph on which techniques and models were used to study ILAs and VP-As, and what new avenues may be opened thanks to more novel methods.
The indirect effect of nonadherence on health-related quality of life in older adults with neurological disorders: implications for clinical endpoints and interventions
Authors: Schönenberg Aline , Mendorf Sarah , Prell Tino
Published: November 2024

Objective

This study assessed how Health-Related Quality of Life (HRQoL) and nonadherence to medication are linked, to determine whether HRQoL is a suitable endpoint for clinical trials evaluating nonadherence.

Background

HRQoL is often used as an endpoint in clinical trials to determine the effectiveness of nonadherence interventions. However, the relationship between HRQoL and nonadherence is not clear, as some interventions find an effect of nonadherence on HRQoL while others do not. Since both HRQoL and nonadherence are latent constructs, it is of interest to understand the factors that link them.

Methods

Medication nonadherence was assessed in 731 older adults with neurological disorders using the Stendal Adherence to Medication Score (SAMS). Regression and network analyses were performed to examine the association between the SAMS and HRQoL (SF-36). Cognitive function, depressive symptoms, mobility, and healthcare satisfaction were included as covariates.

Results

There was a weak association between the SAMS and HRQoL only for the mental component scale. The relationship between the SAMS and HRQoL appears indirect, as its effect is nullified upon the inclusion of covariates, especially depressive symptoms. Network analyses showed that the effect of nonadherence on HRQoL is mainly delivered by depressive symptoms, while cognition and satisfaction with healthcare contribute to a lesser extent.

Conclusion

Nonadherence and HRQoL are both latent variables influenced by similar factors. The effect of nonadherence on HRQoL seems to be indirect and mainly delivered by depressive symptoms, possibly via motivational pathways. These associations need to be considered when selecting clinical endpoints and planning interventions.
Efficacy and safety of anlotinib combined with immune checkpoint inhibitors and platinum-containing chemotherapy for later-line advanced non-small cell lung cancer: a retrospective three-arm real-world study using propensity-score matching
Authors: Wang Zeyang , Ren Bingnan , Yang Haotian , Qiu Xuejia , Wu Yin , Xue Chaojun , Zhao Yue , Li Xiao , Yu Ze , Zhang Jinyuan
Published: November 2024

Objective

To assess the efficacy and safety of anlotinib combined with immune checkpoint inhibitors (ICIs) in patients with advanced non-small-cell lung cancer (NSCLC).

Methods

Clinical data on patients with advanced NSCLC were collected from June 2019 to October 2022 at Hebei General Hospital, China. The efficacy and safety of anlotinib combined with ICIs and platinum-containing chemotherapy were retrospectively analyzed. The primary endpoint was progression-free survival (PFS). The secondary endpoint was the disease control rate (DCR) and overall survival (OS). Survival curves were created using the Kaplan–Meier method. The efficacy and adverse reactions were evaluated according to the RECIST 1.1 and CTCAE 5.0 standards.

Results

A total of 54 patients were enrolled in this study after propensity score matching (PSM), including 27 men and 17 women, with a median age of 59. A total of 26 patients received anlotinib + ICIs + platinum-containing chemotherapy (AIC), 15 patients received anlotinib + platinum-containing chemotherapy (AC), and 13 patients received ICIs + platinum-containing chemotherapy (IC). The PFS of the AIC group was 7.76 months (95% CI: 3.71–NC). The DCR was 65.38%. The OS endpoint had not been reached, The AIC combination regimen group had a significantly longer PFS than the IC group (mPFS, 7.76 vs. 2.33 months, p=0.012, HR=0.23, 95% CI: 0.06–0.8). There was no significant difference in the DCR between the two groups (65.38% vs. 53.85%, p=0.326). There was a statistically significant difference in PFS between the AC group and the IC group (mPFS, 9.2 vs. 2.33 months, p=0.02, HR=0.14, 95% CI: 0.03–0.65). There was no significant difference in the DCR between the two groups (40% vs. 53.85%, p=0.445). The common adverse reactions of the combination of anti-angiogenic agents, ICIs, and platinum-containing chemotherapy were anemia (34.62%), allergic reactions (19.23%), thrombocytopenia (11.54%), gastrointestinal reactions (15.38%), and hepatobiliary disorders (11.54%). Most of them were manageable.

Conclusions

Anlotinib combined with immune checkpoint inhibitors and platinum-containing chemotherapy regimens offers a good survival benefit for patients with advanced non-small-cell lung cancer who fail to respond to standard therapy. When both efficacy and safety are considered, a combination of anti-angiogenic agents, ICIs, and platinum-containing chemotherapy can be used as a choice for the treatment of advanced NSCLC.
Salvage chemotherapy after progression on immunotherapy in recurrent/metastatic squamous cell head and neck carcinoma
Authors: Llop Sandra , Plana Maria , Tous Sara , Ferrando-Díez Angelica , Brenes Jesús , Juarez Marc , Vidales Zara , Vilajosana Esther , Linares Isabel , Arribas Lorena , Duch Maria , Fulla Marta , Brunet Aina , Lozano Alicia , Cirauqui Beatriz , Mesía Ricard , Oliva Marc
Published: November 2024

Objectives

Anti-PD-(L)1 agents changed the landscape of recurrent or metastatic head and neck squamous cell carcinoma (R/M HNSCC) treatment. Previous studies showed improved response rates to salvage chemotherapy (SCT) after progression to anti-PD-(L)1 agents. This study aims to evaluate the outcomes of SCT and to identify predictors of response and survival in patients with R/M HNSCC.

Materials and methods

Retrospective cohort analysis of 63 R/M patients treated with SCT after antiPD-(L1)-based therapy between January 2015 and August 2022. The overall response rate (ORR) was evaluated. Progression-free survival (PFS) and overall survival (OS) were estimated with Kaplan–Meier method. Progression-free survival 2 was calculated from anti-PD-(L)1-therapy start until progression to SCT (PFS2-I). Logistic regression and Cox regression analyses were performed to identify predictors of outcome.

Results

A total of 63 patients were included: 76% were men, and median age was 60 years. PD-L1 status was available in 68% (61% positive). Up to 71% received SCT as third line or beyond. ORR to SCT was 49% with higher rates in PD-L1 positive tumors, 71% vs. 18% (p=0.001), and cetuximab-containing regimens, 68% vs. 39% (p=0.026). PD-L1 status was the only predictor of ORR in the adjusted model (OR=8.6, 95% CI 1.7–43.0). OS and PFS were 9.3 months (95% CI, 6.5–12.3) and 4.1 months (95% CI, 3.0–5.8) respectively. PFS2-I was 8.6 months (95% CI, 6.6–10.5). In the multivariate analysis, PD-L1 was the only independent factor for OS (HR=0.3; 95% CI, 0.1–0.7), PFS (HR=0.2; 95% CI, 0.1–0.5; p<0.001), and PFS2-I (HR=0.2; 95% CI 0.1–0.5; p<0.001).

Conclusion

PDL1 status appeared as a strong predictor of response of efficacy for SCT after anti-PD-(L)1 agents. Patients receiving cetuximab-containing regimens trended towards greater benefit. This highlights the importance of treatment sequencing and personalized treatment strategies.
A novel comprehensive metric balancing imaging dose and setup accuracy in image-guided radiotherapy: concept proposal and clinical validation
Authors: Liu Jiang , Fu Xinhui , Luo Zhiyao , Yin Chuou , Li Qiao , Fan Xigang , Li Tian , Lin Chen , Xu Shouping , Zhang Yibao
Published: November 2024

Purpose

To propose and validate a comprehensive novel metric balancing the registration accuracy and imaging dose for image-guided-radiotherapy based on real patient data.

Materials and methods

With written informed consent and ethical approval, 56 patients were scanned using 6MV CBCT, 140 kV CBCT, and 100 kV CBCT on Halcyon system for three consecutive treatment fractions. Online registration was performed by various on-duty therapists under routine clinical pressure and time limitation. Offline registration was carried out by an experienced physicist without pressure. The consistency between the online and offline results was used as a surrogate of the missing ground-truth of registration accuracy, which was usually developed by introducing ‘known’ setup errors and rescan the phantoms, yet is ethnically not applicable to real patients. The registration differences (ΔD) between various imaging methods and observers were analyzed. The weighted CT dose index (CTDIw) for kV and MV CBCT was acquired using the PTW CTDI head phantom. The weighted-Dose-Accuracy-Product (DAPw) index was defined as DAPw =ΔD(mm) w1* CTDIw(mGy) w2, where w1 and w2 are the weighting factors of accuracy and dose respectively (w1+w2 = 1).

Results

The mean and interquartile range (IQR) of ΔD decreased monotonically for MV CBCT, 100 kV CBCT, and 140 kV CBCT, supporting the registration consistency as a surrogate metric of image quality. Significant differences of ΔD were observed between the online and offline registration across three imaging methods (P<0.05). The 140 kV CBCT provides superior positioning accuracy, less dependency on observer subjectivity and time pressure of clinical workflow. Using w1=w2 = 0.5 as an example, the smallest mean, standard deviation, and IQR of DAPw were observed on the 100 kV CBCT, indicating optimal balance between dose and accuracy than the other two methods. Analysis of variance (ANOVA) showed statistically significant differences in DAPw among the different imaging methods (P<0.01, F=50.57).

Conclusion

Using registration consistency as a surrogate indicator of image quality, this study proposed and validated a novel “DAPw” parameter based on real patient data, providing a purpose-specific tool for balancing setup accuracy and radiation dose in clinic.
Long noncoding RNA mediates enzalutamide resistance and transformation in neuroendocrine prostate cancer
Authors: Zhu Zhe , Xuan Wenjing , Wang Chaohui , Li Chancan
Published: November 2024

Prostate cancer is a malignant tumor caused by the malignant proliferation of epithelial cells, which is highly heterogeneous and drug-resistant, and neuroendocrine prostate cancer (NEPC) is an essential cause of drug resistance in its late stage. Elucidating the evolution of NEPC and the resistance process of enzalutamide, a novel antiandrogen, will be of great help in improving the prognosis of patients. As a research hotspot in the field of molecular biology in recent years, the wide range of biological functions of long noncoding RNAs (lncRNAs) has demonstrated their position in the therapeutic process of many diseases, and a large number of studies have revealed their critical roles in tumor progression and drug resistance. Therefore, elucidating the involvement of lncRNAs in the formation of NEPCs and their interrelationship with enzalutamide resistance may provide new ideas for a deeper understanding of the development of this disease and the occurrence of enzalutamide resistance and give a new direction for reversing the therapeutic dilemma of advanced prostate cancer. This article focuses on lncRNAs that regulate enzalutamide resistance and the neuroendocrine transition of prostate cancer through epigenetic, androgen receptor (AR) signaling, and non-AR pathways that act as “molecular sponges” interacting with miRNAs. Some insights into these mechanisms are used to provide some help for subsequent research in this area.
Potential predictive biomarkers in antitumor immunotherapy: navigating the future of antitumor treatment and immune checkpoint inhibitor efficacy
Authors: Yin Xiangyu , Song Yunjie , Deng Wanglong , Blake Neil , Luo Xinghong , Meng Jia
Published: November 2024

Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment modality, offering promising outcomes for various malignancies. However, the efficacy of ICIs varies among patients, highlighting the essential need of accurate predictive biomarkers. This review synthesizes the current understanding of biomarkers for ICI therapy, and discusses the clinical utility and limitations of these biomarkers in predicting treatment outcomes. It discusses three US Food and Drug Administration (FDA)-approved biomarkers, programmed cell death ligand 1 (PD-L1) expression, tumor mutational burden (TMB), and microsatellite instability (MSI), and explores other potential biomarkers, including tumor immune microenvironment (TIME)-related signatures, human leukocyte antigen (HLA) diversity, non-invasive biomarkers such as circulating tumor DNA (ctDNA), and combination biomarker strategies. The review also addresses multivariable predictive models integrating multiple features of patients, tumors, and TIME, which could be a promising approach to enhance predictive accuracy. The existing challenges are also pointed out, such as the tumor heterogeneity, the inconstant nature of TIME, nonuniformed thresholds and standardization approaches. The review concludes by emphasizing the importance of biomarker research in realizing the potential of personalized immunotherapy, with the goal of improving patient selection, treatment strategies, and overall outcomes in cancer treatment.
Efficacy of neoadjuvant therapy and lymph node dissection in advanced gallbladder cancer without distant metastases: a SEER database analysis
Authors: Dong Jun , Zhu Zhengqiu
Published: November 2024

Purpose

To investigate the effectiveness of neoadjuvant therapy and lymph node dissection(LND) on overall survival (OS) in patients with stage III/IV gallbladder cancer without distant metastases.

Methods

Data from 101 patients who received neoadjuvant therapy followed by surgery combined with adjuvant chemotherapy, and 1412 patients who received direct surgical treatment followed by adjuvant chemotherapy, were collected from the SEER database from 2004 to 2020. Patients were divided into group A (neoadjuvant therapy) and group B (direct surgery) based on the treatment modality. A total of 202 cases were obtained after propensity score matching, with 101 cases in each group (A and B). Cox unifactorial and multifactorial analyses were performed to identify independent risk factors for patients with advanced cholecystic carcinoma, and the Kaplan-Meier method was used to analyze overall survival (OS). The Cox proportional hazards model was used to investigate the effect of different subgroups on OS in both patient groups. Further survival analyses were conducted to determine whether lymph node dissection(LND) was beneficial for patients receiving neoadjuvant therapy for gallbladder cancer.

Results

Cox univariate analysis showed that marital status, AJCC stage, number of LND, tumor size, and treatment modality were associated with OS (P<0.05). Cox multifactorial regression analysis indicated that AJCC stage, LND, tumor size, and treatment modality were independent risk factors for OS in patients with non-metastatic advanced gallbladder cancer (P<0.05). Survival curves demonstrated that the OS in group A was longer than in group B (median OS: 30 months vs. 14 months, P<0.001). Subgroup analysis indicated that neoadjuvant therapy had a consistent effect on the OS of patients with advanced gallbladder cancer, improving both survival time and outcomes. Survival curves indicated that lymph node dissection was not significant in group A patients (p>0.05) but was significant in group B (p<0.05).

Conclusion

Neoadjuvant therapy can improve the OS of patients with non-metastatic stage III/IV gallbladder cancer and is an independent risk factor affecting prognosis; however, the significance of lymph node dissection in these patients still needs further study.
The involvement of the dysfunctional insulin receptor signaling system in long COVID patients with diabetes and chronic pain and its implications for the clinical management using taVNS
Authors: Li Riwang , Liu Wenguo , Liu Dahai , Jin Xu , Wang Shuxing
Published: November 2024

In clinical terms, chronic pain is the most prevalent sequela resulting from COVID-19, which is induced by the novel coronavirus (SARS-CoV-2), while type 2 diabetes mellitus (T2D) is the most common comorbidity. This triangular relationship can be attributed to the dysfunction of the insulin receptor signaling system (IRSS) in both central and peripheral systems. Patients with T2D are essentially more susceptible to SARS-CoV-2 infection due to the widespread expression of angiotensin converting enzyme 2 (ACE2) in their pancreatic beta cells, which serves as the cellular port for the SARS-CoV-2 to infect and enter the cell. This infection can exacerbate chronic pain and insulin resistance for various reasons. Peripherally, once infected, the virus can cause damage to peripheral nerves and pancreatic β-cells, further exacerbating pain and glucose metabolism conditions. Additionally, in the central nervous system, dysfunctional IRSS is closely linked to chronic pain. Over the past few years of the COVID-19 pandemic, an increasing body of evidence suggests that insulin and other medications currently used in clinical practice for hyperglycemia control may not be safe for treating these patients. Therefore, we need a proper approach for the treatment of chronic pain in long COVID patients, especially patients with T2D. This review presents evidence that transcutaneous auricular vagal nerve stimulation (taVNS) may provide a viable treatment option for chronic pain and metabolic dysfunction by improving the function of IRSS in both the central nervous system and peripheral tissues.
Diagnostic and prognostic values of HCG15 and morrbid in acute myocardial infarction
Authors: Huang Min , Wu Bohua , Ou Xiuxia , Sun Shuo , Han Kedong , Li Lijian , Liang Haiyan , Qiu Chunchan , Xu Qingbo
Published: November 2024

Background

Acute myocardial infarction (AMI) represents the gravest manifestation of ischemic heart disease, with the primary cause of mortality and morbidity worldwide. Although timely and accurate diagnosis of AMI is crucial in clinical practice, they are impeded by the limitation of current biomarkers. We aimed to explore the potential predictive value of two novel long non-coding RNA (lncRNA) HCG15 and Morrbid in AMI diagnosis and prognosis.

Method

We measured the lncRNA levels in the blood samples of 412 AMI patients and 111 healthy volunteers with the RT-PCR method. Receiver operating characteristic (ROC) curves were plotted to access the diagnostic value of selected lncRNAs. Restricted cubic splines (RCS) and the Kaplan-Meier method were utilized to examine the predictive value of the selected lncRNAs in AMI diagnosis.

Result

ROC curves identified an acceptable diagnostic value of HCG15 and Morrbid (AUC for HCG15: 0.937; AUC for Morrbid: 0.940). RCS and Kaplan-Meier analysis revealed the cut-off value of 3.6 for HCG15 and 4.0 for Morrbid have a good predictive value in MACCE within 12 months once AMI was diagnosed (p-value for HCG15: p = 0.025; p-value for Morrbid: p < 0.0001).

Conclusion

HCG15 and Morrbid were confirmed as promising lncRNA biomarkers for both diagnosis and prognosis of AMI in this study. Additionally, their importance of application in real-world clinical practice and underlying mechanisms in AMI diagnosis and prognosis remain to be explored.
Velocity structure of crust and mid-strong earthquake preparation characteristics in the Huoshan region, East China
Authors: Hu Tiantian , Fan Xiaoping , He Yicheng , Yang Longshuai , Yang Congjie , Jiang Pengcheng , Liu Zhiwen , Xia Ziyan , Xu Menghui , Wu Yuhan
Published: November 2024

The Huoshan region, located on the northern margin of the Dabie Orogenic Belt at the junction of the North China Plate and the Yangtze Plate, is one of the most seismically active and concentrated areas in the Dabie Orogenic Belt and adjacent regions. Utilizing the travel time data from 4,427 seismic events observed by 202 stations, we investigated the deep medium structure of the Huoshan region using the double-difference tomography method. The results reveal the medium structure and characteristics of mid-strong earthquake preparation in the region. The crustal medium in the study area exhibits significant lateral heterogeneity. The Dabie Orogenic Belt shows notably high velocity, whereas the North China Plate and the Yangtze Plate display relatively lower velocities. The Tan-Lu Fault Zone exhibits segmentation characteristics; with the crustal medium velocity south of Lujiang being relatively high, north of Jiashan being relatively low, and between Lujiang and Jiashan being intermediate. The epicenters of mid-strong earthquakes are located on the gradient zones of velocity and Poisson’s ratio. The source regions of these earthquakes show significant anomalies of high Poisson’s ratio and low S-wave velocity, which may indicate the presence of fluids. These anomalies possibly reflect the intrusion of deep materials along the fault zone, which could be the driving force for the preparation of mid-strong earthquakes.
Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
Authors: Alagna Adriana , Giacalone Vincenzo Maximiliano , Zenone Arturo , Martinez Marco , D’Anna Giovanni , Buffa Gaspare , Cavalca Caterina Jessica , Poli Anna , Varese Giovanna Cristina , Prigione Valeria Paola , Badalamenti Fabio
Published: November 2024

Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. Ad-hoc protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of Posidonia oceanica, the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development. In this study we aim to (i) identify the putative causal agents of seed decay and (ii) test the efficacy of copper sulphate (0.2 and 2 ppm) and of tannin-based products derived from chestnut, tara and quebracho in reducing seed and seedling decay, while assessing possible phytotoxic effects on plant development. Halophytophthora lusitanica, H. thermoambigua and a putative new Halophytophtora species were identified as possible causal agents of seed loss. The antimicrobial agents (copper and tannins) reduced seed contamination by 20%, although copper sulphate at 2 ppm strongly inhibited the root growth. Among tannins, chestnut and tara reduced seeds germination by up to 75% and decreased shoot and root development, while quebracho showed a less severe phytotoxic effect. The use of copper sulphate at 0.2 ppm is therefore recommended to prevent P. oceanica seedling loss in culture facilities since it reduces seed contamination with no phytotoxic effects. Our results contribute to improving the seedling culture of one the key species of the Mediterranean Sea, increasing propagule availability for restoration purposes.
Integrative transcriptomic analysis reveals the molecular responses of tobacco to magnesium deficiency
Authors: Liang Tingmin , Lin Jinbin , Wu Shengxin , Ye Rongrong , Qu Mengyu , Xie Rongrong , Lin Yingfeng , Gao Jingjuan , Wang Yuemin , Ke Yuqin , Li Chunying , Guo Jinping , Lu Jianjun , Tang Weiqi , Chen Songbiao , Li Wenqing
Published: November 2024

Introduction

Magnesium (Mg) is a crucial macronutrient for plants. Understanding the molecular responses of plants to different levels of Mg supply is important for improving cultivation practices and breeding new varieties with efficient Mg utilization.

Methods

In this study, we conducted a comprehensive transcriptome analysis on tobacco (Nicotiana tabacum L.) seedling leaves to investigate changes in gene expression in response to different levels of Mg supply, including Mg-deficient, 1/4-normal Mg, normal Mg, and 4×-normal Mg, with a particular focus on Mg deficiency at 5, 15 and 25 days after treatment (DAT), respectively.

Results

A total of 11,267 differentially expressed genes (DEGs) were identified in the Mg-deficient, 1/4-normal Mg, and/or 4×-normal Mg seedlings compared to the normal Mg seedlings. The global gene expression profiles revealed potential mechanisms involved in the response to Mg deficiency in tobacco leaves, including down-regulation of genes–two DEGs encoding mitochondria-localized NtMGT7 and NtMGT9 homologs, and one DEG encoding a tonoplast-localized NtMHX1 homolog–associated with Mg trafficking from the cytosol to mitochondria and vacuoles, decreased expression of genes linked to photosynthesis and carbon fixation at later stages, and up-regulation of genes related to antioxidant defenses, such as NtPODs, NtPrxs, and NtGSTs.

Discussion

Our findings provide new insights into the molecular mechanisms underlying how tobacco responds to Mg deficiency.
Functional connectivity density of different suicidal behaviors in adolescents with major depressive disorders
Authors: Zhong Hui , Zhang Jianzhao , Mo Daming , Zheng Hongyu , Li Mengting , Liu Wenyuan , Shen Xiaoshuang , Cao Xiaomei , Jia Yanbin
Published: November 2024

Background

Suicidal behavior including suicidal ideation (SI) and suicide attempts (SA) is a common clinical feature of adolescent patients with major depressive disorders (MDD). We hypothesized that differences in functional connectivity density (FCD) exist between adolescent patients with SA and SI, and aimed to investigate the different suicidal behaviors in adolescents patients with MDD17.

Methods

37 MDD adolescents with SA, 34 MDD adolescents with SI, 20 MDD adolescents without SA and SI (non-suicidal group), and 20 adolescents healthy controls (HC) were enrolled in this study. All participants were scanned using functional magnetic resonance imaging (fMRI) to evaluated the FCD. Between-group differences of all variables were analyzed. The relationships between FCD values and clinical scale scores were also analyzed.

Results

The FCD of the left inferior occipital gyrus in the SI group was higher than those in the other groups. The FCD in the SA group was higher than that in the control group. The FCD of the right dorsolateral superior frontal gyrus in the SI group was lower than that in the other three groups. The FCD values of the left precentral gyrus in the SI group were higher than those in the other three groups. The left inferior occipital gyrus FCD positively correlated with the suicide factor score of Hamilton Depression Scale (HAMD), and the right dorsolateral superior frontal gyrus negatively correlated with the HAMD suicide factor score but not with the HAMD total score.

Conclusion

Changes in FCD in adolescent patients with depression and SI can reflect changes in functional connections in the brain.
Toward explainable deep learning in healthcare through transition matrix and user-friendly features
Authors: Barmak Oleksander , Krak Iurii , Yakovlev Sergiy , Manziuk Eduard , Radiuk Pavlo , Kuznetsov Vladislav
Published: November 2024

Modern artificial intelligence (AI) solutions often face challenges due to the “black box” nature of deep learning (DL) models, which limits their transparency and trustworthiness in critical medical applications. In this study, we propose and evaluate a scalable approach based on a transition matrix to enhance the interpretability of DL models in medical signal and image processing by translating complex model decisions into user-friendly and justifiable features for healthcare professionals. The criteria for choosing interpretable features were clearly defined, incorporating clinical guidelines and expert rules to align model outputs with established medical standards. The proposed approach was tested on two medical datasets: electrocardiography (ECG) for arrhythmia detection and magnetic resonance imaging (MRI) for heart disease classification. The performance of the DL models was compared with expert annotations using Cohen’s Kappa coefficient to assess agreement, achieving coefficients of 0.89 for the ECG dataset and 0.80 for the MRI dataset. These results demonstrate strong agreement, underscoring the reliability of the approach in providing accurate, understandable, and justifiable explanations of DL model decisions. The scalability of the approach suggests its potential applicability across various medical domains, enhancing the generalizability and utility of DL models in healthcare while addressing practical challenges and ethical considerations.
Dolomedes fishing spider biology: gaps and opportunities for future research
Authors: Yu Kuang-Ping , Roithmair Zita , Kurovski Jillian , Connolly Simon J. , Vink Cor J. , Johnson James Chadwick , Kralj-Fišer Simona , Kuntner Matjaž , Hebets Eileen A. , Painting Christina J.
Published: November 2024

Dolomedes may easily be considered to be among the most charismatic spider taxa. Known colloquially as fishing or raft spiders, this clade of dolomedid cursorial hunters is speciose with about 100 valid species names. Most Dolomedes are large spiders that inhabit water bodies across all continents except Antarctica and, interestingly, South America. Dolomedes have captured the attention of researchers and the public alike for their ability to walk on and submerge under water, fish for prey (including small vertebrates), and for their often-bizarre mating behavior that includes examples of male spontaneous death and sexual cannibalism. In this review, we critically evaluate what is known of Dolomedes biology, focusing on their systematics and morphology, ecology, behavior, and conservation. Given their close association with water, Dolomedes may be particularly vulnerable to the impacts of anthropogenic change and provide an important group of indicator species for understanding the effect of pollution, habitat loss and climate change. We outline a roadmap for future studies that, in our view, will consolidate Dolomedes as an ideal model lineage among spiders for addressing a vast array of questions across multiple fields of biology.
Canopy height mapping in French Guiana using multi-source satellite data and environmental information in a U-Net architecture
Authors: Lahssini Kamel , Baghdadi Nicolas , le Maire Guerric , Fayad Ibrahim , Villard Ludovic
Published: November 2024

Canopy height is a key indicator of tropical forest structure. In this study, we present a deep learning application to map canopy height in French Guiana using freely available multi-source satellite data (optical and radar) and complementary environmental information. The potential of a U-Net architecture trained on sparse and unevenly distributed GEDI data to generate a continuous canopy height map at a regional scale was assessed. The developed model, named CHNET, successfully produced a canopy height map of French Guiana at a 10-m spatial resolution, achieving relatively good accuracy compared to a validation airborne LiDAR scanning (ALS) dataset. The study demonstrates that relevant environmental descriptors, namely, height above nearest drainage (HAND) and forest landscape types (FLT), significantly contribute to the model’s accuracy, highlighting that these descriptors bring important information on canopy structural properties and that the CHNET framework can efficiently use this information to improve canopy height prediction. Another critical aspect highlighted is the necessity of addressing GEDI data inaccuracies and geolocation uncertainties, which is essential for any GEDI-based application. However, challenges remain, particularly in characterizing tall canopies, as our CHNET model exhibits a tendency to underestimate canopy heights greater than 35 m. A large part of this error arises from the use of GEDI measurements as reference, given the fact these data exhibit certain saturation in tropical biomes. Future improvements in the analysis of GEDI signal as well as the implementation of robust models are essential for better characterization of dense and tall tropical forest ecosystems.
Risk factors associated with human echinococcosis: a systematic review and meta-analysis
Authors: Kakamad Fahmi H. , Anwar Khanda A. , Ahmed Harem K. , Habibullah Imad J. , Kaka Ali Hemn H. , Nasralla Hawkar A. , Abdullah Hiwa O. , Tahir Soran H. , Kareem Honar O. , Hasan Ali H. , Gharib Dana T. , Asaad Hoshmand R. , Mohammed Ayoob A. , Abdalla Berun A. , Esmaeil Deari A. , Rashid Rezheen J. , Hamahussein Karokh F.
Published: November 2024

Introduction

Echinococcosis is a widespread zoonotic disease caused by tapeworms of the Echinococcus genus, manifesting in mature or larval forms. Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are the primary types affecting humans, linked, respectively, to Echinococcus granulosus and Echinococcus multilocularis. This study is a systematic review and meta-analysis of the risk factors associated with CE and AE in humans.

Methods

Relevant English publications were found through a thorough search of eligible databases. The inclusion criteria focused on cross-sectional and case–control studies investigating risk factors for human echinococcosis. Collected data included author, country, study design, demographics, sample size, literacy, occupation, drinking water source, dog ownership, and hand hygiene.

Results

A total of 1,594 studies were found in the initial search, with only 36 papers (involving 1,207,436 cases) meeting the inclusion criteria. Most of the study population (99.35%) showed no echinococcosis infection, while 0.65% were infected. Of the infected cases, 77.92% had CE, while 22.08% had AE. Among 629,996 (52.18%) females, 4,830 (0.76%) were infected, compared to 2,968 (0.52%) infections among 565,872 (46.86%) males (p < 0.001). Rural areas, low education levels, agricultural/livestock workers, dog owners, water sources, and poor hand hygiene were all significantly associated with the infection (p < 0.05).

Conclusion

Echinococcosis remains a global health concern, particularly among rural residents, those with lower education, agricultural workers, and dog owners. Targeted public health measures, including improved hygiene practices and access to clean water, are essential to reducing its impact.
Calculation of Damping Ratio and Analysis of Damping Effect of Turbine Blade Dry Friction Damper in Rotating State
Authors: Li Di , Li Hongguang , Meng Guang , Wei Dasheng , Qiao Kun , Han Le
Published: November 2024

The vibration of turbine blades during the operation of jet engines is a serious and complex issue that has garnered significant attention. In practical jet engines, dry friction damping is commonly used to suppress blade vibrations due to its reliability and efficiency. The equivalent damping ratio of dry friction dampers is a crucial metric for evaluating their performance. However, calculating dry friction dampers’ damping ratio for actual structures involves nonlinear vibration calculations, which are challenging and often lack precision. A method combining simulation and experimentation to calculate the equivalent damping ratio of a structure is proposed. In a laboratory setting, the vibration response of turbine blades under centrifugal load and the damping effect of under-platform dampers were analyzed using oil excitation. The research results indicate that this method can effectively calculate the equivalent damping ratio of actual structures. The findings provide robust support for the design of under-platform dampers and the vibration analysis of turbine blades.
Bacteriophage Therapy as a Promising Alternative for Antibiotic-Resistant Enterococcus faecium: Advances and Challenges
Authors: Ribes-Martínez Laura , Muñoz-Egea Maria-Carmen , Yuste Jose , Esteban Jaime , García-Quintanilla Meritxell
Published: November 2024

Enterococcus faecium is a Gram-positive bacterium increasingly identified as a critical nosocomial pathogen that poses significant treatment challenges due to its resistance to multiple antibiotics, particularly vancomycin-resistant E. faecium (VRE) strains. The urgent need for alternative therapeutic strategies has renewed interest in bacteriophage (phage) therapy, given phages specificity and bactericidal potential. This review explores the advancements in phage therapy against antibiotic-resistant E. faecium, including phage morphological diversity, genomic characteristics, and infection mechanisms. The efficacy of phage therapy in in vitro, ex vivo, and in vivo models and the compassionate use in clinical settings are evaluated, highlighting the promising outcomes of phage–antibiotic synergies and biofilm disruption. Key challenges and future research directions are discussed, with a focus on improving therapeutic efficacy and overcoming bacterial resistance. This review emphasizes the potential of phage therapy as a viable solution for managing multidrug-resistant E. faecium infections and underscores the importance of future investigations to enhance clinical applications.
Infective Endocarditis by Biofilm-Producing Methicillin-Resistant Staphylococcus aureus—Pathogenesis, Diagnosis, and Management
Authors: Kaushik Ashlesha , Kest Helen , Sood Mangla , Thieman Corey , Steussy Bryan W. , Padomek Michael , Gupta Sandeep
Published: November 2024

Infective endocarditis (IE) is a life-threatening condition with increasing global incidence, primarily caused by Staphylococcus aureus, especially methicillin-resistant strains (MRSA). Biofilm formation by S. aureus is a critical factor in pathogenesis, contributing to antimicrobial resistance and complicating the treatment of infections involving prosthetic valves and cardiovascular devices. Biofilms provide a protective matrix for MRSA, shielding it from antibiotics and host immune defenses, leading to persistent infections and increased complications, particularly in cases involving prosthetic materials. Clinical manifestations range from acute to chronic presentations, with complications such as heart failure, embolic events, and neurological deficits. Diagnosis relies on the Modified Duke Criteria, which have been updated to incorporate modern cardiovascular interventions and advanced imaging techniques, such as PET/CT (positron emission tomography, computed tomography), to improve the detection of biofilm-associated infections. Management of MRSA-associated IE requires prolonged antimicrobial therapy, often with vancomycin or daptomycin, needing a combination of antimicrobials in the setting of prosthetic materials and frequently necessitates surgical intervention to remove infected prosthetic material or repair damaged heart valves. Anticoagulation remains controversial, with novel therapies like dabigatran showing potential benefits in reducing thrombus formation. Despite progress in treatment, biofilm-associated resistance poses ongoing challenges. Emerging therapeutic strategies, including combination antimicrobial regimens, bacteriophage therapy, antimicrobial peptides (AMPs), quorum sensing inhibitors (QSIs), hyperbaric oxygen therapy, and nanoparticle-based drug delivery systems, offer promising approaches to overcoming biofilm-related resistance and improving patient outcomes. This review provides an overview of the pathogenesis, current management guidelines, and future directions for treating biofilm-related MRSA IE.
Three-Dimensional Active Magnetic Levitation Actuating and Control System for Curved Pipes
Authors: Liu Guancheng , Gao Meng , Sun Deshuai , Jiang Renjun , Fan Lei
Published: November 2024

A three-dimensional active maglev (magnetic levitation) actuating system based on force imbalance is proposed. By combining the principle of force imbalance control with the control algorithm, the stable levitation and controllable levitating motion of the magnetic ball can be realized. The four electromagnetic actuating structures are used to stabilize the force of the controlled object, and the dual-hall sensor group and hardware differential method are used to improve control stability and accuracy. By combining the fine adjustment of the active maglev actuating system with the coarse adjustment of the mechanical arm, the three-dimensional levitation motion of the magnetic ball in curved pipes is realized. Experimental results show that the proposed control algorithm solves problems such as the increase of deviation between the controlled object and the steady-state operating point and the rapid deterioration of tracking performance in the model-based control method. In the vertical direction, the overshoot is within 0.418%, regardless of axis motion or non-axis motion. In the horizontal direction, the offset limits left and right of the axis are 4.590 mm and 3.536 mm, respectively. The fluctuation of vertical and horizontal motion is within the allowable range of ±0.2 mm. This can be applied to the non-destructive quality detection of the inner walls and the internal dredging of long and thin pipes in examinations and industrial fields.
Investigation of the Influence of Lubricating Oil Viscosity on the Wear-Reducing Characteristics of Cylinder Liner Surface Texture
Authors: Zhang Hongyang , Gong Junzhen , Ma Yuejin , Sun Wen , Sun Ke , Bai Shuzhan
Published: November 2024

Lubricating oil and cylinder liner surface textures can significantly reduce the friction coefficient between the piston ring and the cylinder liner, thereby improving engine performance. However, the friction-diminishing mechanisms between the lubricating oil and surface texture remain unclear. Properly combining lubricating oil and surface texture can achieve better friction reduction effects. This paper, based on a transient thermo-hydrodynamic model developed in MATLAB 2020a, conducted numerous simulation experiments to explore the matching characteristics of textured cylinder liners. The study provides theoretical support for the future selection of lubricating oils for textured cylinder liners. The results show that, within the range of the circular texture parameters used in this study, the texture radius is directly proportional to the reduction in friction mean effective pressure (FMEP), while the texture depth is inversely proportional to the FMEP reduction. At the same rotational speed, as the viscosity of the lubricating oil increases, the friction-reducing effect of the texture on the piston ring–cylinder liner pair decreases. When the texture depth is 2 μm, the engine speed is inversely proportional to the reduction in FMEP. As the texture depth increases from 2 μm to 6 μm, there is a significant change in the friction-reducing effect: for the 2 μm texture, the friction-reducing impact decreases with increasing lubricant viscosity, while for the 6 μm texture, the friction-reducing effect increases with increasing lubricant viscosity.