bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Dosing Regimen of Osteoporosis Drugs in Relation to Food Intake as the Key for the Enhancement of the Treatment Effectiveness—A Concise Literature Review
Authors: Wiesner Agnieszka , Szuta Mariusz , Galanty Agnieszka , Paśko Paweł
Published: March 2021

Bisphosphonates and selective estrogen receptor modulators (SERMs) represent the two most important groups of medications taken orally and employed in osteoporosis treatment. Effectiveness of the therapy may be affected by poor patient adherence, in particular, due to the inconvenient dosing regimen of oral bisphosphonates. With this review we aimed to assess the effects that food, beverages, and dietary supplements consumed during treatment, along with the dosing regimens, may have on pharmacokinetics and pharmacodynamics of oral drugs employed in treating osteoporosis; we also aimed to shape the recommendations valuable for professional patients’ counseling and education, to provide appropriate dosing regimens in order to improve adherence to the therapy. Food, beverages such as coffee, juices, and mineral water, as well as dietary supplements containing multivalent cations, e.g., calcium, magnesium, aluminium, iron, showed to have a deleterious effect on the bioavailability of all the investigated oral bisphosphonates, specifically alendronate, risedronate, ibandronate, minodronate, and etidronate. For risedronate, a delayed-release (DR) tablet was designed to solve the malabsorption problem in the presence of food, hence DR risedronate can be ingested following breakfast. For other oral bisphosphonates, the proper interval between drug and food, beverages, and dietary supplements intake should be maintained to minimize the risk of interactions. The effect of food on pharmacokinetic parameters of selective estrogen receptor modulators (SERMs) was found to be clinically irrelevant.
Water Management of River Beaches—A Portuguese Case Study
Authors: Oliveira Maria , Antunes Margarida , Carvalho Ana
Published: March 2021

The quality of water is crucial for the qualification of river beaches. The Cávado River watershed (Northern Portugal) contains five river beaches with a regular and specific mandatory monitorization. The main subject of this research is the evaluation of spatial and temporal water microbiological and physicochemical parameters to assess the water quality improvement and consequently watershed management. The results of monitoring surface water, considering microbiological parameters from the five river beaches (2015/19), and physicochemical parameters from three water points along the Cávado River (2018/19) were considered. The river beaches located upstream of the town of Braga has an “excellent” and “good” quality, while the river beach located downstream shows a lower water quality. The physicochemical water results indicated that there is a progressive degradation of water quality from upstream to downstream of the river, which is associated with the influence of domestic and industrial activities. To improve water quality, continuous monitoring will be necessary, with the implementation of adequate awareness-raising programs and strategic water quality management by the population and local agents.
SUMAMOS EXCELENCIA® Project: Results of the Implementation of Best Practice in a Spanish National Health System (NHS)
Authors: Martínez-Gimeno María-Lara , Fernández-Martínez Nélida , Escobar-Aguilar Gema , Moreno-Casbas María-Teresa , Brito-Brito Pedro-Ruyman , Caperos Jose-Manuel
Published: March 2021

The use of certain strategies for the implementation of a specific recommendation yields better results in clinical practice. The aim of this study was to assess the effectiveness of an evidence-based model using clinical audits (GRIP model), for the implementation of recommendations in pain and urinary incontinence management as well as fall prevention, in the Spanish National Health System during the period 2015–2018. A quasi-experimental study has been conducted. The subjects were patients treated in hospitals, primary care units and nursing home centers. There were measures related to pain, fall prevention and urinary incontinence. Measurements were taken at baseline and at months 3, 6, 9, and 12. The sample consisted of 22,114 patients. The frequency of pain assessment increased from 59.9% in the first cycle to a mean of 71.6% in the last cycle, assessments of risk of falling increased from 56.8% to 87.8% in the last cycle; and finally, the frequency of assessments of urinary incontinence increased from a 43.4% in the first cycles to a mean of 62.2% in the last cycles. The implementation of specific evidence-based recommendations on pain, fall prevention, and urinary incontinence using a model based on clinical audits improved the frequency of assessments and their documentation.
Prevalence and Associated Factors of Frailty and Mortality in Patients with End-Stage Renal Disease Undergoing Hemodialysis: A Systematic Review and Meta-Analysis
Authors: Lee Hyeon-Ju , Son Youn-Jung
Published: March 2021

Hemodialysis is the most common type of treatment for end-stage renal disease (ESRD). Frailty is associated with poor outcomes such as higher mortality. ESRD patients have a higher prevalence of frailty. This systematic review and meta-analysis aimed to identify the prevalence and associated factors of frailty and examine whether it is a predictor of mortality among ESRD patients undergoing hemodialysis. Five electronic databases including PubMed, Embase, CINAHL, Web of Science, and Cochrane Library were searched for relevant studies up to 30 November 2020. A total of 752 articles were found, and seven studies with 2604 participants in total were included in the final analysis. The pooled prevalence of frailty in patients with ESRD undergoing hemodialysis was 46% (95% Confidence interval (CI) 34.2−58.3%). Advanced age, female sex, and the presence of diabetes mellitus increased the risk of frailty in ESRD patients undergoing hemodialysis. Our main finding showed that patients with frailty had a greater risk of all-cause mortality compared with those without (hazard ratio (HR): 2.02, 95% CI: 1.65−2.48). To improve ESRD patient outcomes, healthcare professionals need to assess the frailty of older ESRD patients, particularly by considering gender and comorbidities. Comprehensive frailty screening tools for ESRD patients on hemodialysis need to be developed.
The Seneb’s Enigma: Impact of a Hybrid Personal and Social Responsibility and Gamification Model-Based Practice on Motivation and Healthy Habits in Physical Education
Authors: Melero-Canas David , Manzano-Sánchez David , Navarro-Ardoy Daniel , Morales-Baños Vicente , Valero-Valenzuela Alfonso
Published: March 2021

Increasing physical activity (PA) and personal and social values are two of the greatest demands in the current educational system. This study examined the effects of a program based on the hybridization of the Personal and Social Responsibility Model and gamification. A total of 58 students (13.89 years old, SD = 1.14) in two groups (experimental and control) participated during a complete academic year. Motivation, physical activity and sedentary behavior were assessed through questionnaires. Physical fitness was evaluated using previously validated field tests. The results showed significant differences over time between the experimental group (EG) and control group (CG) in afterschool physical activity (APA) during the weekend (p = 0.003), sedentary time (p = 0.04) and speed–agility (p = 0.04). There were no significant differences in motivation. In reference to the intervention, the speed–agility (p = 0.000), strength (p = 0.000), agility (p = 0.000), cardiorespiratory fitness (p = 0.001), APA–weekend (p = 0.000), APA–week (p = 0.000) and sedentary time (p = 0.000) increased significantly in the EG. The speed–agility (p = 0.000), APA–weekend (p = 0.03) and sedentary time (p = 0.008) increased in the CG. The implementation of a program based on the hybridization of pedagogical models can be useful in producing improvements in physical fitness, physical activity and sedentary behaviors.
A Case for Systematic Quality Management in Mosquito Control Programmes in Europe
Authors: Michaelakis Antonios , Balestrino Fabrizio , Becker Norbert , Bellini Romeo , Caputo Beniamino , della Torre Alessandra , Figuerola Jordi , L’Ambert Gregory , Petric Dusan , Robert Vincent , Roiz David , Saratsis Anastasios , Sousa Carla A. , Wint William G. R. , Papadopoulos Nikos T.
Published: March 2021

The recent spread of invasive mosquito species, such as Aedes albopictus and the seasonal sporadic transmission of autochthonous cases of arboviral diseases (e.g., dengue, chikungunya, Zika) in temperate areas, such as Europe and North America, highlight the importance of effective mosquito-control interventions to reduce not only nuisance, but also major threats for public health. Local, regional, and even national mosquito control programs have been established in many countries and are executed on a seasonal basis by either public or private bodies. In order for these interventions to be worthwhile, funding authorities should ensure that mosquito control is (a) planned by competent scientific institutions addressing the local demands, (b) executed following the plan that is based on recommended and effective methods and strategies, (c) monitored regularly by checking the efficacy of the implemented actions, (d) evaluated against the set of targets, and (e) regularly improved according to the results of the monitoring. Adherence to these conditions can only be assured if a formal quality management system is adopted and enforced that ensures the transparency of effectiveness of the control operation. The current paper aims at defining the two components of this quality management system, quality assurance and quality control for mosquito control programs with special emphasis on Europe, but applicable over temperate areas.
Muscle Oxygen Desaturation and Re-Saturation Capacity Limits in Repeated Sprint Ability Performance in Women Soccer Players: A New Physiological Interpretation
Authors: Vasquez-Bonilla Aldo A. , Camacho-Cardeñosa Alba , Timón Rafael , Martínez-Guardado Ismael , Camacho-Cardeñosa Marta , Olcina Guillermo
Published: March 2021

Muscle oxygen consumption could provide information on oxidative metabolism in women soccer players. Therefore, the objective of this study was to analyze muscle oxygenation dynamics during repeated sprint ability (RSA): (8 sprint × 20 s recovery) by near-infrared spectroscopy (NIRS). The sample was made up of 38 professional women soccer players. To measure the external load, the best time, worst time, average time, individual speed, sprint decrement, and power were assessed. In connection with the internal load, the desaturation (sprint) and re-saturation (recovery) rates, as well as the oxygen extraction (∇%SmO2) in the gastrocnemius muscle and maximum heart rate (%HRmax) were measured. A repeated measures statistic was applied based on the inter-individual response of each subject from the baseline versus the other sprints, with linear regression and nonlinear regression analyses between variables. There was an increase in the SmO2: desaturation rate after four sprints (Δ = 32%), in the re-saturation rate after six sprints (Δ = 89%), and in ∇%SmO2 after four sprints (Δ = 72.1%). There was a linear association between the rates of desaturation and re-saturation relationships and the worst time (r = 0.85), and a non-linear association between ∇%SmO2 and speed (r = 0.89) and between ∇%SmO2 and the sprint decrease (r = 0.93). The progressive increase in SmO2 during RSA is a performance limitation to maintain a high speed; it depends on the capacity of fatigue resistance. Therefore, monitoring the muscle oxygenation dynamics could be a useful tool to evaluate the performance in women soccer players.
Children’s Perceived and Actual Physical Activity Levels within the Elementary School Setting
Authors: Howells Kristy , Coppinger Tara
Published: March 2021

To date, little research has longitudinally examined young children’s physical activity (PA) during school hours, nor questioned children’s perceptions of their own PA behaviours. This study investigated 20 children’s actual physical activity levels (APA) and their perceived physical activity levels (PPA) (10 infants, mean age 6.6 years; 10 juniors, mean age 9.5 years). APA was evaluated using accelerometers across 36 whole school days (371 min per day); 18 days included Physical Education (PE) lessons and 18 did not. A repeated-measures three-factor ANOVA analysed: type of day; age phase; parts of the day and sex. PPA was collected by an interactive handset and an adapted version of the PA Questionnaire for Children (PAQ-C). Participants undertook 10 more minutes of moderate to vigorous PA (MVPA) on PE days (53 ± 19 min) compared to non-PE days (43 ± 15 min) (F = 92.32, p < 0.05) and only junior boys reached daily MVPA recommendations (60 ± 13 min) on PE days. Juniors over-estimated, and infants under-estimated, their APA levels. Educators need more support to teach and embed different PA intensities into the school day to enable children to better understand the health benefits associated with varying the intensity of their PA during school hours.
Bayesian Group Index Regression for Modeling Chemical Mixtures and Cancer Risk
Authors: Wheeler David C. , Rustom Salem , Carli Matthew , Whitehead Todd P. , Ward Mary H. , Metayer Catherine
Published: March 2021

There has been a growing interest in the literature on multiple environmental risk factors for diseases and an increasing emphasis on assessing multiple environmental exposures simultaneously in epidemiologic studies of cancer. One method used to analyze exposure to multiple chemical exposures is weighted quantile sum (WQS) regression. While WQS regression has been demonstrated to have good sensitivity and specificity when identifying important exposures, it has limitations including a two-step model fitting process that decreases power and model stability and a requirement that all exposures in the weighted index have associations in the same direction with the outcome, which is not realistic when chemicals in different classes have different directions and magnitude of association with a health outcome. Grouped WQS (GWQS) was proposed to allow for multiple groups of chemicals in the model where different magnitude and direction of associations are possible for each group. However, GWQS shares the limitation of WQS of a two-step estimation process and splitting of data into training and validation sets. In this paper, we propose a Bayesian group index model to avoid the estimation limitation of GWQS while having multiple exposure indices in the model. To evaluate the performance of the Bayesian group index model, we conducted a simulation study with several different exposure scenarios. We also applied the Bayesian group index method to analyze childhood leukemia risk in the California Childhood Leukemia Study (CCLS). The results showed that the Bayesian group index model had slightly better power for exposure effects and specificity and sensitivity in identifying important chemical exposure components compared with the existing frequentist method, particularly for small sample sizes. In the application to the CCLS, we found a significant negative association for insecticides, with the most important chemical being carbaryl. In addition, for children who were born and raised in the home where dust samples were taken, there was a significant positive association for herbicides with dacthal being the most important exposure. In conclusion, our approach of the Bayesian group index model appears able to make a substantial contribution to the field of environmental epidemiology.
Vitamin D Levels Are Reduced at the Time of Hospital Admission in Sicilian SARS-CoV-2-Positive Patients
Authors: Gaudio Agostino , Murabito Andrea , Agodi Antonella , Montineri Arturo , Castellino Pietro ,   D.O.CoV Research
Published: March 2021

The coronavirus disease 2019 (COVID-19) pandemic poses a worldwide healthcare challenge that needs an efficient response. Unfortunately, to date there is no highly effective treatment, so a deep understanding of COVID-19 risk factors could be an important step in treating the disease. Vitamin D affects the immune system in many different ways, and other authors already found that COVID-19 patients have low levels of vitamin D. In our retrospective study, we evaluated the vitamin D status at the time of hospital admission in 50 COVID-19 patients in Sicily, which is the southernmost region of Italy, and compared them with 100 control subjects matched for age and sex. Our data showed markedly low levels of vitamin D in patients with a positive polymerase chain reaction (PCR) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but no association was found with inflammation markers or clinical severity. Vitamin D levels were reduced at the time of hospital admission in Sicilian SARS-CoV-2-positive patients, but it is not clear whether this condition has an impact on the clinical course of COVID-19.
Bayesian Methods for Meta-Analyses of Binary Outcomes: Implementations, Examples, and Impact of Priors
Authors: Al Amer Fahad M. , Thompson Christopher G. , Lin Lifeng
Published: March 2021

Bayesian methods are an important set of tools for performing meta-analyses. They avoid some potentially unrealistic assumptions that are required by conventional frequentist methods. More importantly, meta-analysts can incorporate prior information from many sources, including experts’ opinions and prior meta-analyses. Nevertheless, Bayesian methods are used less frequently than conventional frequentist methods, primarily because of the need for nontrivial statistical coding, while frequentist approaches can be implemented via many user-friendly software packages. This article aims at providing a practical review of implementations for Bayesian meta-analyses with various prior distributions. We present Bayesian methods for meta-analyses with the focus on odds ratio for binary outcomes. We summarize various commonly used prior distribution choices for the between-studies heterogeneity variance, a critical parameter in meta-analyses. They include the inverse-gamma, uniform, and half-normal distributions, as well as evidence-based informative log-normal priors. Five real-world examples are presented to illustrate their performance. We provide all of the statistical code for future use by practitioners. Under certain circumstances, Bayesian methods can produce markedly different results from those by frequentist methods, including a change in decision on statistical significance. When data information is limited, the choice of priors may have a large impact on meta-analytic results, in which case sensitivity analyses are recommended. Moreover, the algorithm for implementing Bayesian analyses may not converge for extremely sparse data; caution is needed in interpreting respective results. As such, convergence should be routinely examined. When select statistical assumptions that are made by conventional frequentist methods are violated, Bayesian methods provide a reliable alternative to perform a meta-analysis.
Quadratus Lumborum and Transversus Abdominis Plane Blocks and Their Impact on Acute and Chronic Pain in Patients after Cesarean Section: A Randomized Controlled Study
Authors: Borys Michał , Zamaro Aleksandra , Horeczy Beata , Gęszka Ewa , Janiak Marek , Węgrzyn Piotr , Czuczwar Mirosław , Piwowarczyk Paweł
Published: March 2021

Background: Severe postoperative pain is a significant problem after cesarean sections. Methods: This study was a randomized, controlled trial of 105 patients conducted in two hospitals. All patients were anesthetized spinally for elective cesarean section. Each participant was randomly allocated to one of three study groups: the quadratus lumborum block (QLB) group, the transversus abdominis plane block (TAPB) group, or the control (CON) group. The primary outcome of this study determined acute pain intensity on the visual analog scale (VAS). The secondary outcomes determined morphine consumption and chronic pain evaluation according to the Neuropathic Pain Symptom Inventory (NPSI) after hospital discharge. Results: At rest, the pain intensity was significantly higher in the CON group than in the QLB and TAPB groups at hours two and eight. Upon activity, the pain in the control subjects was more severe than in the QLB and TAPB groups in three and two of five measurements, respectively. Moreover, morphine consumption was significantly lower in the QLB (9 (5–10)) and TAPB (10 (6–14)) groups than in the CON (16 (11–19)) group. Persistent postoperative pain was significantly lower in the QLB group than in the CON group at months one and six following hospital discharge. Conclusions: Both the QLB and TAPB can improve pain management after cesarean delivery. Moreover, the QLB might reduce the severity of persistent postoperative pain months after cesarean section.
Engagement of Government Social Media on Facebook during the COVID-19 Pandemic in Macao
Authors: Pang Patrick Cheong-Iao , Cai Qixin , Jiang Wenjing , Chan Kin Sun
Published: March 2021

Government social media is widely used for providing updates to and engaging with the public in the COVID-19 pandemic. While Facebook is one of the popular social media used by governments, there is only a scant of research on this platform. This paper aims to understand how government social media should be used and how its engagement changes in prodromal, acute and chronic stages of the pandemic. We collected 1664 posts and 10,805 comments from the Facebook pages of the Macao government from 1 January to 31 October 2020. Using word frequency and content analysis, the results suggest that the engagement was relatively low at the beginning and then surged in the acute stage, with a decreasing trend in the chronic stage. Information about public health measures maintained their engagement in all stages, whereas the engagement of other information was dropping over time. Government social media can be used for increasing vigilance and awareness in the prodromal stage; disseminating information and increasing transparency in the acute stage; and focusing on mental health support and recovery policies in the chronic stage. Additionally, it can be a tool for controlling rumors, providing regular updates and fostering community cohesion in public health crises.
Addendum: Rudasill, K.M.; et al. Promoting Higher Quality Teacher–Child Relationships: The INSIGHTS Intervention in Rural Schools. Int. J. Environ. Res. Public Health 2020, 17, 9371
Authors: Rudasill Kathleen Moritz , Reichenberg Ray E. , Eum Jungwon , Barrett Jentry Stoneman , Joo Yuenjung , Wilson Emily , Sealy Martinique
Published: March 2021

The authors wish to make the following correction to this paper [...]
Strategies to Increase Uptake of Parent Education Programs in Preschool and School Settings to Improve Child Outcomes: A Delphi Study
Authors: Sim Wan Hua , Toumbourou John W. , Clancy Elizabeth M. , Westrupp Elizabeth M. , Benstead Michelle L. , Yap Marie B. H.
Published: March 2021

Parent education programs, offered via family–school partnerships, offer an effective means for promoting the mental health and educational functioning of children and adolescents at a whole-school level. However, these programs often have a low uptake. This study aimed to identify strategies for increasing the uptake of parent education programs within preschool and school settings. A three-round Delphi procedure was employed to obtain expert consensus on strategies that are important and feasible in educational settings. First, thirty experts rated statements identified from the literature and a stakeholder forum. Next, experts re-appraised statements, including new statements generated from the first round. Ninety statements were endorsed by ≥80% of the experts. Primary themes include strategies for program selection; strategies for increasing the accessibility of programs and the understanding of educational staff on parent engagement and child mental health; strategies for program development, promotion and delivery; as well as strategies for increasing parent and community engagement. This study offers a set of consensus strategies for improving the uptake of parent education programs within family–school partnership.
Assessing Hotel Employee Knowledge on Risk Factors and Risk Management Procedures for Microbial Contamination of Hotel Water Distribution Systems
Authors: Leftwich Brandon , Opoku Samuel , Yin Jingjing , Adhikari Atin
Published: March 2021

Water management plans (WMPs), sometimes referred to as risk management plans (RMPs) or water safety plans (WSPs), are not mandatory for hotels in many countries of the world, including the US. As such, many hotel personnel are uninformed of WMPs and the precautions to take if their hotel water system is compromised. The purpose of this study was to identify hotel personnel’s knowledge and practices of WMPs through a survey incorporating the Health Belief Model (HBM). Data were collected from 59 hotels within Fulton County, Georgia, USA, through a questionnaire, and questions were developed tailored to the HBM. Significant associations were found between the perceived susceptibility of contracting a waterborne illness and WMP for hotel personnel as well as between cues to action and having a WMP in general linear models (p ≤ 0.05). The study concludes that many key personnel are not aware of WMPs. Many hotel facilities do not have a plan in place, and some facilities are unaware of a current plan is in place. The study findings provide insight into the importance of WMPs and the risk factors associated with microbial contamination in a hotel building’s plumbing system. Future research and potential law change should be emphasized to increase hotel employees’ and owner’s WMP knowledge.
Parent-Adolescent Communication, School Engagement, and Internet Addiction among Chinese Adolescents: The Moderating Effect of Rejection Sensitivity
Authors: Li Jingjing , Yu Chengfu , Zhen Shuangju , Zhang Wei
Published: March 2021

Although a large body of research has indicated that parent-adolescent communication is a crucial protective factor for adolescent Internet addiction, the mediating and moderating mechanisms underlying this relationship remain unclear. To address this research gap, this study, based on social control theory and the organism-environment interaction model, was designed to test whether school engagement mediated the relationship between parent-adolescent communication and adolescent Internet addiction and whether this mediating effect was moderated by rejection sensitivity. A sample of 1006 adolescents (Meanage = 13.16 years, SD = 0.67) anonymously completed the questionnaires. The results showed that the positive association between parent-adolescent communication and adolescent Internet addiction was mediated by school engagement. Moreover, this indirect link was stronger among adolescents with high rejection sensitivity than those with low rejection sensitivity. These findings highlighted school engagement as a potential mechanism linking parent-adolescent communication to adolescent Internet addiction, with high rejection sensitivity being an important risk factor amplifying this indirect effect. Intervention programs aimed at reducing Internet addiction among adolescents might benefit from the current research.
The Factors Associated with Attempted Smoking Cessation and Successful Four-Week Smoking Abstinence According to the Types of Disability in Seoul, Korea
Authors: Kang Han-Nu-Ri , Lee Kang-Sook , Koh JuYeon , Park YuJin , Shin HyunKyung
Published: March 2021

This study investigated smoking behaviors by disability type among people with disabilities in Korea and identified factors associated with attempted smoking cessation and successful four-week smoking abstinence. Data were collected between 1 January 2018 and 31 December 2019. Predictors of attempted smoking cessation and successful four-week smoking abstinence were analyzed by disability type in 557 participants. Compared to people with mental health disorders, people with physical disabilities or brain lesions were more likely to attempt smoking cessation, and people with physical or internal disabilities were more likely to successfully abstain for four weeks. Common predictors of smoking cessation attempts and four-week abstinence were education level and CO level. Employment status predicted attempted cessation, while confidence in smoking cessation predicted four-week abstinence. To provide effective smoking cessation services for people with disabilities, disability type should be considered, and comprehensive and sustainable community-based programs need to be developed. Furthermore, a standardized survey of people with disabilities should be conducted to examine socioeconomic factors, including health status, employment, and education level, and to explore fundamental measures needed to address the problem of smoking among people with disabilities.
Barriers of Occupational Safety Implementation in Infrastructure Projects: Gaza Strip Case
Authors: Abu Aisheh Yazan Issa , Tayeh Bassam A. , Alaloul Wesam Salah , Jouda Amro Fareed
Published: March 2021

Infrastructure projects are the foundation for essential public services and have an influential position in societal development. Although the role of infrastructure projects is substantial, they can involve complexities and safety issues that lead to an unsafe environment, and which impacts the project key stakeholders. Therefore, this study aimed to evaluate the barriers to implementing occupational safety in infrastructure projects in the Gaza Strip, which cause serious threats and reduce project performance. To evaluate the barriers, 39 items were highlighted and modified as per the construction context and environment, and which later were distributed in the form of a questionnaire, to get feedback from consultants and contractors. The analysis shows that in the safety policy barriers group, consultants and contractors both ranked the item “a contractor committed to an occupational safety program is not rewarded” first. In the management barriers group, consultants and contractors both ranked the item “safety engineer does not have significant powers, such as stopping work when needed” in the first place. In the behavior and culture barriers group, consultants and contractors both ranked the item “workers who are not committed to occupational safety are not excluded” in the first place. Overall, both consultants and contractors shared the same viewpoint in classifying the barriers in the working environment. The outcome of this study is beneficial for Palestinian construction industry policymakers, so they can monitor the highlighted barriers in on-going infrastructure projects and can modify the safety guidelines accordingly.
Decision Making in the 4th Dimension—Exploring Use Cases and Technical Options for the Integration of 4D BIM and GIS during Construction
Authors: Liu Alyssa Huaqiu , Ellul Claire , Swiderska Monika
Published: March 2021

In both the Geospatial (Geo) and Building Information Modelling (BIM) domains, it is widely acknowledged that the integration of geo-data and BIM-data is beneficial and a crucial step towards solving the multi-disciplinary challenges of our built environment. The result of this integration—broadly termed GeoBIM—has the potential to be particularly beneficial in the context of the construction of large infrastructure projects, which could make use of data relating to the larger spatial extents typically handled in geographical information systems (GIS) as well as the detailed models generated by BIM. To date, GeoBIM integration has mainly been explored for buildings, in a 3D context and for small projects. This paper demonstrates the results of the next level of integration, exploring the addition of the fourth dimension by linking project schedule information to create 4D GeoBIM, examining interoperability challenges and benefits in the context of a number of use cases relating to the enabling works for a major commercial infrastructure project. The integrating power of location and time—knowing where and when data relate to—allows us to explore data interoperability challenges relating to linking real world construction data, created using commercial software, with other data sources; we are then able to demonstrate the benefits of 4D GeoBIM in the context of three decision making scenarios: examining the potential for prioritisation of noise mitigation interventions by identifying apartments closest to the noisiest construction process; development of a 4D location-enabled risk register allowing, for example, work to continue underground if a risk is specific to the top of a building; ensuring construction safety by using 3D buffering to ensure that the required distances between moving construction equipment and surrounding infrastructure are not breached. Additionally, once integrated, we are able to ‘democratize’ the data—make it accessible beyond the BIM and GIS expert group—by embedding it into a 3D/4D open source Web GIS tool.
Proliferative Capacity of Adult Mouse Brain
Authors: Semenov Mikhail
Published: March 2021

We studied cell proliferation in the postnatal mouse brain between the ages of 2 and 30 months and identified four compartments with different densities of proliferating cells. The first identified compartment corresponds to the postnatal pallial neurogenic (PPN) zone in the telencephalon; the second to the subpallial postnatal neurogenic (SPPN) zone in the telencephalon; the third to the white matter bundles in the telencephalon; and the fourth to all brain parts outside of the other three compartments. We estimated that about 3.4 million new cells, including 0.8 million in the subgranular zone (SGZ) in the hippocampus, are produced in the PPN zone. About 21 million new cells, including 10 million in the subependymal zone (SEZ) in the lateral walls of the lateral ventricle and 2.7 million in the rostral migratory stream (RMS), are produced in the SPPN zone. The third and fourth compartments together produced about 31 million new cells. The analysis of cell proliferation in neurogenic zones shows that postnatal neurogenesis is the direct continuation of developmental neurogenesis in the telencephalon and that adult neurogenesis has characteristics of the late developmental process. As a developmental process, adult neurogenesis supports only compensatory regeneration, which is very inefficient.
Candidate Genes for the High-Altitude Adaptations of Two Mountain Pine Taxa
Authors: Zaborowska Julia , Łabiszak Bartosz , Perry Annika , Cavers Stephen , Wachowiak Witold
Published: March 2021

Mountain plants, challenged by vegetation time contractions and dynamic changes in environmental conditions, developed adaptations that help them to balance their growth, reproduction, survival, and regeneration. However, knowledge regarding the genetic basis of species adaptation to higher altitudes remain scarce for most plant species. Here, we attempted to identify such corresponding genomic regions of high evolutionary importance in two closely related European pines, Pinus mugo and P. uncinata, contrasting them with a reference lowland relative—P. sylvestris. We genotyped 438 samples at thousands of single nucleotide polymorphism (SNP) markers, tested their genetic differentiation and population structure followed by outlier detection and gene ontology annotations. Markers clearly differentiated the species and uncovered patterns of population structure in two of them. In P. uncinata three Pyrenean sites were grouped together, while two outlying populations constituted a separate cluster. In P. sylvestris, Spanish population appeared distinct from the remaining four European sites. Between mountain pines and the reference species, 35 candidate genes for altitude-dependent selection were identified, including such encoding proteins responsible for photosynthesis, photorespiration and cell redox homeostasis, regulation of transcription, and mRNA processing. In comparison between two mountain pines, 75 outlier SNPs were found in proteins involved mainly in the gene expression and metabolism.
Impairments of Photoreceptor Outer Segments Renewal and Phototransduction Due to a Peripherin Rare Haplotype Variant: Insights from Molecular Modeling
Authors: Donato Luigi , Abdalla Ebtesam Mohamed , Scimone Concetta , Alibrandi Simona , Rinaldi Carmela , Nabil Karim Mahmoud , D'Angelo Rosalia , Sidoti Antonina
Published: March 2021

Background: Retinitis pigmentosa punctata albescens (RPA) is a particular form of retinitis pigmentosa characterized by childhood onset night blindness and areas of peripheral retinal atrophy. We investigated the genetic cause of RPA in a family consisting of two affected Egyptian brothers with healthy consanguineous parents. Methods: Mutational analysis of four RPA causative genes was realized by Sanger sequencing on both probands, and detected variants were subsequently genotyped in their parents. Afterwards, found variants were deeply, statistically, and in silico characterized to determine their possible effects and association with RPA. Results: Both brothers carry three missense PRPH2 variants in a homozygous condition (c.910C > A, c.929G > A, and c.1013A > C) and two promoter variants in RHO (c.-26A > G) and RLBP1 (c.-70G > A) genes, respectively. Haplotype analyses highlighted a PRPH2 rare haplotype variant (GAG), determining a possible alteration of PRPH2 binding with melanoregulin and other outer segment proteins, followed by photoreceptor outer segment instability. Furthermore, an altered balance of transcription factor binding sites, due to the presence of RHO and RLBP1 promoter variants, might determine a comprehensive downregulation of both genes, possibly altering the PRPH2 shared visual-related pathway. Conclusions: Despite several limitations, the study might be a relevant step towards detection of novel scenarios in RPA etiopathogenesis.
CRISPR-Cas9–Mediated TIM3 Knockout in Human Natural Killer Cells Enhances Growth Inhibitory Effects on Human Glioma Cells
Authors: Morimoto Takayuki , Nakazawa Tsutomu , Matsuda Ryosuke , Nishimura Fumihiko , Nakamura Mitsutoshi , Yamada Shuichi , Nakagawa Ichiro , Park Young-Soo , Tsujimura Takahiro , Nakase Hiroyuki
Published: March 2021

Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor in adults. Natural Killer (NK) cells are potent cytotoxic effector cells against tumor cells inducing GBM cells; therefore, NK cell based- immunotherapy might be a promising target in GBM. T cell immunoglobulin mucin family member 3 (TIM3), a receptor expressed on NK cells, has been suggested as a marker of dysfunctional NK cells. We established TIM3 knockout in NK cells, using the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). Electroporating of TIM3 exon 2- or exon 5-targeting guide RNA- Cas9 protein complexes (RNPs) inhibited TIM3 expression on NK cells with varying efficacy. T7 endonuclease I mutation detection assays showed that both RNPs disrupted the intended genome sites. The expression of other checkpoint receptors, i.e., programmed cell death 1 (PD1), Lymphocyte-activation gene 3 (LAG3), T cell immunoreceptor with Ig and ITIM domains (TIGIT), and TACTILE (CD96) were unchanged on the TIM3 knockout NK cells. Real time cell growth assays revealed that TIM3 knockout enhanced NK cell–mediated growth inhibition of GBM cells. These results demonstrated that TIM3 knockout enhanced human NK cell mediated cytotoxicity on GBM cells. Future, CRISPR-Cas9 mediated TIM3 knockout in NK cells may prove to be a promising immunotherapeutic alternative in patient with GBM.
Expression Pattern of α-Tubulin, Inversin and Its Target Dishevelled-1 and Morphology of Primary Cilia in Normal Human Kidney Development and Diseases
Authors: Solic Ivana , Racetin Anita , Filipovic Natalija , Mardesic Snjezana , Bocina Ivana , Galesic-Ljubanovic Danica , Glavina Durdov Meri , Saraga-Babić Mirna , Vukojevic Katarina
Published: March 2021

The spatiotemporal expression of α-tubulin, inversin and dishevelled-1 (DVL-1) proteins associated with the Wnt-signaling pathway, and primary cilia morphology were analyzed in developing kidneys (14th–38th developmental weeks), healthy postnatal (1.5- and 7-years old) and pathologically changed human kidneys, including multicystic dysplastic kidneys (MCDK), focal segmental glomerulosclerosis (FSGS) and nephrotic syndrome of the Finnish type (CNF). The analysis was performed by double immunofluorescence, electron microscopy, semiquantitative and statistical methods. Cytoplasmic co-expression of α-tubulin, inversin and DVL-1 was observed in the proximal convoluted tubules (pct), distal convoluted tubules (dct) and glomeruli (g) of analyzed tissues. During kidney development, the overall expression of α-tubulin, inversin and DVL-1 decreased, while in the postnatal period slightly increased. The highest expressions of α-tubulin and inversin characterized dct and g, while high DVL-1 characterized pct. α-tubulin, inversin and DVL-1 expression pattern in MCDK, FSGS and CNF kidneys significantly differed from the healthy control. Compared to healthy kidneys, pathologically changed kidneys had dysmorphic primary cilia. Different expression dynamics of α-tubulin, inversin and DVL-1 during kidney development could indicate that switch between the canonical and noncanonical Wnt-signaling is essential for normal kidney morphogenesis. In contrast, their disturbed expression in pathological kidneys might be associated with abnormal primary cilia, leading to chronic kidney diseases.
Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis
Authors: Roseren Flavy , Pithioux Martine , Robert Stéphane , Balasse Laure , Guillet Benjamin , Lamy Edouard , Roffino Sandrine
Published: March 2021

Granulocyte colony-stimulating factor (G-CSF) was shown to promote bone regeneration and mobilization of vascular and osteogenic progenitor cells. In this study, we investigated the effects of a systemic low dose of G-CSF on both bone consolidation and mobilization of hematopoietic stem/progenitor cells (HSPCs), endothelial progenitor cells (EPCs) and mesenchymal stromal cells (MSCs) in a rat model of distraction osteogenesis (DO). Neovascularization and mineralization were longitudinally monitored using positron emission tomography and planar scintigraphy. Histological analysis was performed and the number of circulating HSPCs, EPCs and MSCs was studied by flow cytometry. Contrary to control group, in the early phase of consolidation, a bony bridge with lower osteoclast activity and a trend of an increase in osteoblast activity were observed in the distracted callus in the G-CSF group, whereas, at the late phase of consolidation, a significantly lower neovascularization was observed. While no difference was observed in the number of circulating EPCs between control and G-CSF groups, the number of MSCs was significantly lower at the end of the latency phase and that of HSPCs was significantly higher 4 days after the bone lengthening. Our results indicate that G-CSF accelerates bone regeneration and modulates mobilization of progenitor cells during DO.
Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase
Authors: Mussa Bashair M. , Khan Amir Ali , Srivastava Ankita , Abdallah Sallam Hasan
Published: March 2021

It is evident that depletion of interstitial cells and dysfunction of nitric oxide (NO) pathways are key players in development of several gastrointestinal (GI) motility disorders such as diabetic gastroparesis (DGP). One of the main limitations of DGP research is the lack of isolation methods that are specific to interstitial cells, and therefore conducting functional studies is not feasible. The present study aims (i) to differentiate telomerase transformed mesenchymal stromal cells (iMSCs) into platelet-derived growth factor receptor-α-positive cells (PDGFRα-positive cells) using connective tissue growth factor (CTGF) and L-ascorbic acids; (ii) to investigate the effects of NO donor and inhibitor on the survival rate of differentiated PDGFRα-positive cells; and (iii) to evaluate the impact of increased glucose concentrations, mimicking diabetic hyperglycemia, on the gene expression of neuronal nitric oxide synthase (nNOS). A fibroblastic differentiation-induction medium supplemented with connective tissue growth factor was used to differentiate iMSCs into PDGFRα-positive cells. The medium was changed every day for 21 days to maintain the biological activity of the growth factors. Gene and protein expression, scanning electron and confocal microscopy, and flow cytometry analysis of several markers were conducted to confirm the differentiation process. Methyl tetrazolium cell viability, nitrite measurement assays, and immunostaining were used to investigate the effects of NO on PDGFRα-positive cells. The present study, for the first time, demonstrated the differentiation of iMSCs into PDGFRα-positive cells. The outcomes of the functional studies showed that SNAP (NO donor) increased the survival rate of differentiated PDGFRα-positive cells whereas LNNA (NO inhibitor) attenuated these effects. Further experimentations revealed that hyperglycemia produced a significant increase in expression of nNOS in PDGFRα-positive cells. Differentiation of iMSCs into PDGFRα-positive cells is a novel model to conduct functional studies and to investigate the involvement of NO pathways. This will help in identifying new therapeutic targets for treatment of DGP.
Developmental and Neurotoxicity of Acrylamide to Zebrafish
Authors: Park Jong-Su , Samanta Palas , Lee Sangwoo , Lee Jieon , Cho Jae-Woo , Chun Hang-Suk , Yoon Seokjoo , Kim Woo-Keun
Published: March 2021

Acrylamide is a commonly used industrial chemical that is known to be neurotoxic to mammals. However, its developmental toxicity is rarely assessed in mammalian models because of the cost and complexity involved. We used zebrafish to assess the neurotoxicity, developmental and behavioral toxicity of acrylamide. At 6 h post fertilization, zebrafish embryos were exposed to four concentrations of acrylamide (10, 30, 100, or 300 mg/L) in a medium for 114 h. Acrylamide caused developmental toxicity characterized by yolk retention, scoliosis, swim bladder deficiency, and curvature of the body. Acrylamide also impaired locomotor activity, which was measured as swimming speed and distance traveled. In addition, treatment with 100 mg/L acrylamide shortened the width of the brain and spinal cord, indicating neuronal toxicity. In summary, acrylamide induces developmental toxicity and neurotoxicity in zebrafish. This can be used to study acrylamide neurotoxicity in a rapid and cost-efficient manner.