bluuebunny/crossref_metadata_2025 huggingface dataset:
Attitude of the youth in the Republic of Tatarstan towards organ donation
Authors: Anisimov A. A. , Gilmetdinova E. S. , Mulendeeva M. A. , Anisimov A. Yu.
Published: April 2022

Objective: to study the views of the youth in the Republic of Tatarstan on organ donation and transplantation, to analyze their awareness and ideas about donor transplants and potential their willingness to become organ donors.

Materials and methods. An anonymous sociological survey of 880 respondents aged 18 to 35 from the Republic of Tatarstan was conducted in the period from January 1 to July 1, 2021. An 11-question questionnaire was developed using online service Google Forms. Participation in the survey was voluntary.

Results. Female and male respondents accounted for 79.0% and 21.0%, respectively; 34.2% of the respondents have or are receiving medical education. Among the respondents, 71.5% have a clear understanding of the term «organ donation», 27.4% are not sure of their understanding, 1.1% do not have a clear understanding. 56.8% consider the issue of organ donation for transplantation in the Republic of Tatarstan as a pressing matter, 3.5% do not see it so, while 39.7% found it difficult to answer. After death, 35.9% would agree to become donors, 39.5% probably would agree, 9.3% probably would disagree, 5.6% strongly disagrees, 9.7% found it difficult to answer. The most common associations with organ donation were positive: 34.5% associate it with «life», 25.1% with «help», and 22.0% with «lifeline».

Conclusion. Young people in the Republic of Tatarstan are ready for a healthy debate of the problem of organ donation and most of them see it as a noble course. Given the interest in the problem and lack of awareness by the target audience, it is advisable to include independent academic disciplines on transplantology and organ donation in the curriculums of medical universities in the country. It is necessary to attract modern interdisciplinary information and educational resources to promote organ donation among the Russian public.
History and background of kidney transplantation in Uzbekistan
Authors: Matkarimov Z. T. , Bahritdinov F. S. , Ibadov R. A. , Suyumov A. S. , Mahmudov Q. O. , Ahmedov A. R. , Shernazarov S. I. , Rustamov M. O. , Abdugafurov Z. U. , Saatova U. M. , Urinov J. B.
Published: April 2022

This paper presents a brief outline of the history of transplantation service in the Republic of Uzbekistan, which originated at the country’s Center for Kidney Transplantation. The role played by outstanding scientists in Uzbekistan, their works and efforts towards the creation of a separate area of clinical and scientific medicine in Uzbekistan, are highlighted. Achievements by the research school of U.A. Aripov, Academician of the Academy of Sciences of the Republic of Uzbekistan, who performed the first successful kidney transplantation in 1972, are shown. The ups and downs of national transplantation nephrology, as well as the birth of a national school of kidney transplantation, domiciled at the Vakhidov Republican Specialized Scientific and Practical Medical Center of Surgery, headed by academician F.G. Nazirov, and giving a stimulus to the «second breath» of the national school of transplantology, is reflected. Separate attention is devoted to the actual problems of national transplantology, moral, ethical and regulatory issues that inevitably accompany this scientific and clinical direction are reflected. Kidney transplant outcomes in Uzbekistan are given, the prospects for further scientific and clinical directions are indicated.
Determining the optimal pancreatic decellularization protocol, taking into account tissue morphological features
Authors: Ponomareva A. S. , Baranova N. V. , Kirsanova L. A. , Bubentsova G. N. , Nemets E. A. , Miloserdov I. A. , Sevastianov V. I.
Published: April 2022

Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves a search for matrices/scaffolds capable of mimicking the structure and composition of the natural extracellular matrix (ECM), which is an important component of the tissue microenvironment. A cell-free, tissue-specific matrix obtained from pancreas decellularization seems to be the most suitable for creation of a TEPC. The choice of pancreatic tissue decellularization protocol should take into account the morphological characteristics of the original pancreas. Preservation of the architectonics and composition of the native tissue in the decellularized pancreas matrix (DPM), and the presence of native ECM components allow for creation of conditions for prolonged vital activity of functionally active islet (insulin-producing) cells when creating TEPC.

Objective: to determine the optimal parameters for decellularization of deceased donor pancreas with fibrosis, lipomatosis, and without pronounced signs of fibrosis and lipomatosis.

Materials and methods. We used the caudal part of the pancreas obtained after multiorgan procurement from deceased donors, which was unsuitable for transplantation. Tissue-specific matrix was obtained by a combination of physical and chemical methods of pancreatic decellularization. A freeze-thaw cycle protocol and two protocols using osmotic shock were used. Samples of initial pancreatic tissue and decellularized fragments were subjected to histological analysis.

Results. It was shown that a physico-chemical method with freeze-thaw cycles is suitable for effective pancreatic decellularization in severe lipomatosis; a physico-chemical method using osmotic shock, but different protocol variants, is suitable for pancreas with diffuse fibrosis and for pancreas without pronounced signs of fibrosis and lipomatosis.

Conclusion. For complete human pancreatic decellularization, the protocol should be correlated with histological features of the original tissue.
Combined treatment of unresectable hilar cholangiocarcinoma with subsequent liver transplantation
Authors: Granov D. A. , Tileubergenov I. I. , Zhuikov V. N. , Sheraliev A. R. , Polikarpov A. A. , Moiseenko A. V.
Published: April 2022

Objective: to demonstrate the experience of unresectable hilar cholangiocarcinoma treatment using neoadjuvant therapy followed by liver transplantation (LT).

Materials and methods. From 2017 to 2021, six patients were included in the treatment protocol for unresectable Klatskin tumor followed by liver transplantation at Granov Russian Scientific Center for Radiology and Surgical Technology. The neoadjuvant therapy included endobiliary photodynamic therapy (PDT), as well as regional and systemic chemotherapy. Each method was used at least three times for 4 to 5 months with radiological evaluation and measurement of CA 19-9 levels. Patients were placed on the waiting list when the tumor marker reduced, or when there were no radiological signs of disease progression and there was no acute cholangitis. The recipients underwent laparoscopic abdominal revision for carcinomatosis and assessment of lymph nodes in the hepatoduodenal ligament with urgent morphological examination. Where there was no extrahepatic spread, LT was performed according to the classical technique with paracaval, para-aortic and hepatoduodenal lymphodissection, biliodigestive anastomosis by an isolated Roux loop of small intestine. The operation was performed in three patients, all of them were men aged 40 to 55 years (mean 48). The mean time from the start of treatment to transplantation was 9.3 months (range 6 to 14). Mean CA 19-9 level at the time of intervention was 81.3 IU/mL (8 to 212).

Results. In three patients, CA 19-9 levels more than doubled on average over four months despite treatment. According to data from computed tomography RECIST assessment, two of the patients showed disease progression. In one patient, carcinomatosis was detected by diagnostic laparoscopy. In three patients, CA 19-9 levels decreased more than fourfold. Two of these patients were radiologically confirmed to have the disease stabilized, and one had a partial response. One patient died from sepsis three years after transplantation as a result of secondary biliary cirrhosis and biliary abscesses without signs of progression. Two patients are still alive after 6 and 21 months without signs of tumor progression.

Conclusion. LT for unresectable Klatskin tumor is effective in controlling the bioactivity of the tumor through the use of neoadjuvant therapy
Effect of transdermal immunomodulation on liver regeneration
Authors: Kuznetsova E. G. , Kuryleva O. M. , Salomatina L. A. , Kirsanova L. A. , Gonikova Z. Z. , Nikolskaya A. O. , Shmerko N. P. , Sevastianov V. I
Published: April 2022

Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.

Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.

Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).

Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.
From diagnosis to complications: experiences of those who live with systemic lupus erythematosus
Authors: Souza Rebeca Rosa de , Marcon Sonia Silva , Teston Elen Ferraz , Barreto Mayckel da Silva , Reis Pamela dos , Cecilio Hellen Pollyanna Mantelo , Marquete Verônica Francisqueti , Ferreira Patricia Chatalov
Published: April 2022

ABSTRACT Objective: To understand how people with lupus experience the diagnosis and how they deal with complications arising from the disease. Method: Qualitative study, whose data were collected between February and July 2019, through semi-structured interviews with 26 individuals and submitted to content analysis. Results: Three categories emerged that show illness from lupus as a difficult experience, permeated by sadness, fear and suffering, which, in addition to being linked to society’s lack of knowledge about the disease, negatively impacts the lives of those who experience it. Furthermore, they show that the time of living with the disease favors the development of self-care strategies and greater therapeutic adherence and, consequently, longer periods of disease remission. Considerations: More disclosure about the disease and its implications in the daily lives of those affected is essential, culminating in greater understanding of family, friends and colleagues and improvements in health care and quality of life for these people.
Validation of Questionnaire to Assess the Impact of Dementia on the Family
Authors: Silva Mafalda Sofia Gomes Oliveira da , Sá Luís Octávio de , Almeida Sandra Sofia Barbosa Pinto de , Reis Ana Catarina Rodrigues da Silva
Published: April 2022

ABSTRACT Objective: To validate a questionnaire to assess the impact of dementia on one of the household members. Methods: Methodological study. The instrument was designed based on literature review, expert opinion, and researchers’ experience and then applied to a non-probability convenience sample consisting of 262 family members who live daily with a person with dementia. The construct validity was studied by exploratory factor analysis, principal components method, with varimax rotation of the items. Results: An instrument with 30 items was obtained, distributed in four dimensions: “Emotional dimension,” “Economic dimension,” “Family relations dimension,” and “Support-seeking dimension.” Factor analysis revealed a total explained variance of 54.96% and a total Cronbach’s alpha of .899. Conclusions: The instrument presents high internal consistency, grouped into four dimensions, all closely related to the family’s adaptation to the onset of dementia in one of its members.
Official COVID-19 coping strategies in educational institutions
Authors: Strapasson Sabrina , Khalaf Daiana Kloh , Macedo Laura Christina , Pypcak Everly Maltaca , Lima Amanda Thais , Mendonça Roseli Camargo , Moraes Juliana Vieira de
Published: April 2022

ABSTRACT Objective: To analyze normative acts on coping with COVID-19 in the teaching environment, sanctioned by the state and federal governments of Brazil. Methods: Documentary research, with a qualitative approach to documents published by federal and state education and health bodies between March/2020 and March/2021, analyzed according to Bardin’s Document Analysis framework. Results: From 61 documents, four categories resulted: Acts and strategies to protect against COVID19 adopted by the federal government; Normative acts of state governments on suspension of classes; Strategies to protect against COVID-19 in the teaching environment common among Brazilian states; Strategies to protect against COVID-19 in the teaching environment specific to Brazilian states. Final considerations: The normative acts analyzed present weaknesses regarding the clarification on how to establish the systematization of COVID-19 surveillance actions in the teaching environment, as well as there is a lack of reflections on the importance of such actions.
The family system seeking to transform its eating behavior in the face of childhood obesity
Authors: Verga Samea Marine Pimentel , Mazza Verônica de Azevedo , Teodoro Fernanda Cassanho , Girardon-Perlini Nara Marilene Oliveira , Marcon Sonia Silva , Rodrigues Érika Tatiane de Almeida Fernandes , Ruthes Victoria Beatriz Trevisan Nóbrega Martins
Published: April 2022

ABSTRACT Objectives: to understand families’ behaviors and eating practices in the face of childhood obesity. Methods: a qualitative study, which used the Grounded Theory as a methodological framework and the Complexity Theory as a theoretical framework. Twenty-six informants participated in the study, who were part of two sample groups. Data were collected through intensive interviews, using a semi-structured script. Results: “The family system seeking to change eating behavior patterns in the face of childhood obesity” emerged as a central concept, relating three conceptual categories: “Recognizing its behavior patterns and eating practices”; “Reorganizing in the face of childhood obesity”; “Responding to change”. Final Considerations: the family influences children’s eating behavior and contributes to changes that occur in it, which highlights the relevance of the family approach in childhood obesity care, raising reflection on the current nursing practice together with families who experience the same problem.
Let it not be that nursing that asks for silence: participation in social movements and sociopolitical-emancipatory knowledge
Authors: Rabelo Ana Renata Moura , Silva Kênia Lara da
Published: April 2022

ABSTRACT Objectives: to analyze the expression of sociopolitical-emancipatory knowledge, based on the participation of women-nurses in social movements, and the implications for nursing care. Methods: a research-interference, whose data were obtained from narrative interviews with six women-nurses inserted in social movements and with political representation. Data were submitted to discourse analysis, based on Michel Foucault. Results: women-nurses’ social and political involvement is driven by the contexts of life and work, marked by gender inequalities. Ability to criticize oneself, the profession and health policies and practices are presented as expressions of sociopolitical-emancipatory knowledge. Nursing care is conceived as a political practice that, influenced by learning from social movements, must go through a denial of the historically performed form. Final Considerations: participation in social movements triggers sociopolitical-emancipatory knowledge, resulting in differentiated care, a way of acting oriented towards reducing inequalities.
Risk factors for Central Nervous System drug use among nursing students
Authors: Abreu Victórya Suéllen Maciel , Teles Davi Oliveira , Rodrigues Hillary Bastos Vasconcelos , Pires José Mateus , Soares Paula Renata Amorim Lessa , Aquino Priscila de Souza , Ribeiro Samila Gomes
Published: April 2022

ABSTRACT Objectives: to verify sociodemographic, academic and health risk factors for Central Nervous System drug use among nursing students. Methods: a cross-sectional study with 199 public undergraduate students from Ceará, using Characterization Instrument, Antonovsky’s Sense of Coherence Questionnaire, Beck Depression Inventory and Beck Anxiety Inventory. Fischer’s exact test and Pearson’s chi-square test were performed, verifying an association between variables. Prevalence ratio was used. Results: a total of 34 used such drugs, with predominance of use of anxiolytics, associated with age (p=0.026), dissatisfaction with the relationship between classmates (p=0.003), insomnia (p=0.009), chronic disease (p=0.001), depression (p=0.035), and severe anxiety (p=0.023). Conclusions: using Central Nervous System drugs among students was associated with sociodemographic, academic and health factors, evidencing the need for actions promoting undergraduate students’ mental health.
Organizational climate and nurses’ turnover intention: a mixed method study
Authors: Bracarense Carolina Feliciana , Costa Nara dos Santos , Raponi Maria Beatriz Guimarães , Goulart Bethania Ferreira , Chaves Lucieli Dias Pedreschi , Simões Ana Lúcia de Assis
Published: April 2022

ABSTRACT Objectives: to describe the organizational climate perceived by nurses of a hospital linked to the Brazilian Hospital Services Company and the reasons for the turnover intention. Methods: mixed, concomitant triangulation type. Qualitative data were analyzed according to the discourse of the collective subject, in addition to quantitative data analysis, descriptive analysis, Pearson correlation, and multiple linear regression. Data collection was carried through a semi-structured interview with 116 nurses. Results: the study presented the speeches in five categories: Leadership and organization support; Reward; Physical comfort; Control/pressure; and Cohesion among colleagues. It showed that, with increased Reward factor, there is a decrease in turnover intention; and, with decreased Physical comfort, there is an increase in turnover intention. Conclusions: there is a dichotomy in the organizational climate perceived by nurses, and personal reasons mainly justify the turnover intention. The reason could be the current processes of institutional reorganization and the hiring of experienced staff.
National Systems of Innovation and Non-OECD Countries: Notes about a rudimentary and tentative “typology”
Authors: ALBUQUERQUE EDUARDO DA MOTTA E
Published: April 2022

ABSTRACT This paper suggests a tentative “typology” for Non-OECD National Systems of Innovation (NSIs). Three main categories compose this tentative “typology”: “mature” NSIs, catching up NSIs, and “non-mature” NSIs. This paper investigates theoretical mediations which may be necessary if the NSI concept is to be applied appropriately to Non-OECD countries. Science and technology statistics are used to evaluate the suggested “typology”. Basic statistics (GDP, R&D expenditures, education, patents, and papers) are presented. Data from forty-six countries are used for statistical exercises. The findings of this paper hint that it is possible to cluster different countries around science and technology indicators.
UNDENATURED COLLAGEN TYPE II FOR THE TREATMENT OF OSTEOARTHRITIS OF THE KNEE
Authors: SADIGURSKY DAVID , MAGNAVITA VICTOR FILARDI STOLZE , SÁ CLOUD KENNEDY COUTO DE , MONTEIRO HENRIQUE DE SOUSA , BRAGHIROLI ODDONE FREITAS MELRO , MATOS MARCOS ANTÔNIO ALMEIDA
Published: April 2022

ABSTRACT Objective: To test the hypothesis that undenatured type II collagen (UC-II) relieves pain, quality of life, and joint function in women aged from 60 to 80 years with knee osteoarthritis. Methods: 53 patients in the UC-II treatment group (for 90 days) and 52 in the control group (without UC-II) were evaluated at 1, 30, and 90 days regarding health-related quality of life, pain, and function with questionnaires, anthropometric data, alignment, range of motion, and radiographic analysis. Results: Quality of life increased significantly in the Physical domain in the treatment vs control group. Also, there was a difference between the first and the last evaluation on the pain visual analog scale (−3.8 ± 1.8 versus −1.3 ± 2.0) and on the WOMAC score (−9.5 ± 11.9 versus −1.3 ± 11.1). No variation in the temporal evolution of the Mental domain was found. Conclusion: Pain, joint stiffness, and quality of life (Physical domain) improved with the inclusion of UC-II for 90 days to the therapeutic toolbox for knee osteoarthritis in individuals aged 60 to 80 years. Level of evidence II, Comparative Prospective Study.
RESULT OF BONE BLOCKING SURGERY IN COMBAT ATHLETES WITH ANTERIOR SHOULDER INSTABILITY: A PROSPECTIVE STUDY
Authors: BALDAN ARTHUR RODRIGUES , PEREIRA VITOR LUIS , LARA PAULO HENRIQUE SCHMIDT , EJNISMAN BENNO , BELANGERO PAULO SANTORO
Published: April 2022

ABSTRACT Objective: This study aims to understand the way fighting athletes respond to bone block surgery in the treatment for shoulder instability. Methods: Prospective clinical study with competitive fighters with shoulder instability who underwent bone block surgery from 2013 to 2016, followed by a postoperative rehabilitation protocol. For the evaluation, eight combat athletes with anterior shoulder instability were treated, with a total of nine shoulders, since one athlete underwent bilateral surgery. All patients signed the Free and Informed Consent Form. The evaluation protocol included medical consultation, radiography of the operated shoulder, degree of active and passive lateral rotation; degree of active and passive elevation; visual analogue scale (VAS) for pain; Athletic Shoulder Outcome Rating Scale (EROE; acronym in Portuguese) scores; Western Ontario Shoulder Instability Index (WOSI), and American Shoulder and Elbow Surgeons (ASES). Results: We observed a decrease in the range of passive and active movement in the recent postoperative period. In later postoperative, values were close to those in the preoperative period at the end of the follow-up. There was improvement in pain, and in all ASES, WOSI and EROE scores no complications were documented. As for returning to sport, two athletes did not return, one of them due to shoulder pain and the other due to retirement. Conclusion: Bone block surgery has shown good functional results in uncomplicated combat athletes. Level of Evidence IV, Prospective Case Series.
THE EFFECT OF THE USE OF ALCOHOL AND DRUGS ON MOTORCYCLICAL TRAUMAS WITH SURGICAL TREATMENT FRACTURES: EPIDEMIOLOGY
Authors: PIRES FERNANDO ARAUJO , SANTOS JOÃO BAPTISTA GOMES DOS , UETA RENATO HIROSHI SALVIONI , FALOPPA FLÁVIO , MORAES VINICIUS YNOE DE , TAMAOKI MARCEL JUN SUGAWARA
Published: April 2022

ABSTRACT Objective: To verify the prevalence of patients who were victims of motorcycle trauma who were under the influence of alcohol and drugs. Methods: The study was observational and prospective, with patients hospitalized to the Orthopedics and Traumatology Ward of Hospital São Paulo (UNIFESP), from March 2015 to March 2016. The study included patients of all genders, over 18 years old, hospitalized due to motorcycle trauma and who needed orthopedic surgical treatment. Results: During the research, 282 patients were hospitalized, of which 23.8% were victims of motorcycle trauma. Of these, 49.3% motorcyclists reported the use of alcohol and drugs before the accident, while 50.7% denied their consumption. In the analysis of alcohol and drug consumption in patients with motorcycle injuries, the results showed that: 65% used only alcohol; 16% used both alcohol and drugs; and 19% used only illicit drugs. Conclusion: The incidence of patients who suffered motorcycle accidents under the influence of alcohol and drugs was 49.3%. Level of Evidence IV, Prospective Case Series.
EXPERIMENTAL MODEL STUDY OF ISCHEMIC NECROSIS INDUCTION OF THE GROWING FEMORAL HEAD
Authors: GORIOS FILHO ANTONIO , SANTOS GUSTAVO BISPO DOS , GUARNIERO JOSÉ ROBERTO BEVILACQUA , ANGELI LUIZ RENATO AGRIZZI DE , GRANGEIRO PATRICIA MORENO , GUARNIERO ROBERTO
Published: April 2022

ABSTRACT Many experimental models exist to better understand the necrosis of the femoral head etiology, both in terms of the species variety in which necrosis is induced and in the operative techniques used for treatment. Objective: This study has two main objectives, the first is to review the literature concerning experimental models of avascular necrosis of the growing femoral head, the second, to demonstrate the experimental pig model’s reproducibility using a pilot study. Methods: This was a bibliographic review to describe the attempts over time to find the best species and technique for induction that would reproduce ischemic necrosis of the growing femoral head in humans. Simultaneously, a pilot study was performed to verify the replication of induction in pigs, the species that has more similarities with the human hip. The pilot’s methodological analysis consists of conventional radiology and verification of possible anatomical, pathological changes. Results: In imaging exams; lateral sub-dislocation of the femur head and triangular appearance of the head were observed, characterizing its flattening; in macroscopic examination, the femoral head flattening with femoral neck widening and shortening was identified; in histology, the proliferation of articular cartilage with the presence of vascular granulation regenerative tissue, with osteoclasts and fibrocartilaginous tissue in the metaphyseal femoral neck region was identified. Conclusion: The experimental pig model can be used as a valuable tool for the reproducibility of anatomical, pathological changes in ischemic necrosis of the growing femoral head. The model is reproducible and feasible and can be beneficial for future studies on the anatomical pathology of necrosis of the growing femoral head. Level of Evidence III, Literature Review .
Insecticidal activity of essential oils on Spodoptera frugiperda and selectivity to Euborellia annulipes
Authors: Alves A. C. L. , Silva T. I. , Batista J. L. , Galvão J. C. C.
Published: April 2022

Abstract Fall armyworm (Spodoptera frugiperda) is the main species that causes damage to the maize crop in Brazil. In the perspective of studying alternatives of control of this pest that preserve the natural enemies, the aim of this research was to evaluate the insecticidal efficiency of the essential oils of Vanillosmopsis arborea and Lippia microphylla on S. frugiperda and verify the selectivity to the predator Euborellia annulipes. The bioassays were carried out in the Agricultural Entomology Laboratory of the Federal University of Paraiba, using insects, from 3rd instar of S. frugiperda and E. annulipes, originating from mass rearing in the laboratory itself. Dilutions of the oils were performed in Tween® 80 at concentrations of 0, 100, 150 and 200 mg mL-1. 1.0 μL from each dilution was applied to the prothoracic region of the insects. The S. frugiperda mortality was verified by topical contact of V. arborea oil with LC10 = 74.3 mg mL-1 and LC50 = 172.86 mg mL-1, for L. microphyla, LC10 = 51.26 mg mL-1 and LC50 = 104.52 mg mL-1. The observed lethal concentrations for E. annulipes were V. arborea LC10 = 71.3 mg mL-1 and LC50 = 160.2 mg mL-1. While L. microphyla, were LC10 = 50.3 mg mL-1 and LC50 = 134.67 mg mL-1. The essential oils of V. arborea and L. microphylla are efficient in the control of S. frugiperda, but are not selective to the predator E. Annulipes.
USO DE ESTREPTOMICINA NA ELIMINAÇÃO DA LEPTOSPIRÚRIA EM TOUROS (BOS TAURUS INDICUS) NATURALMENTE INFECTADOS PELO SOROVAR HARDJO
Authors: Girio T.M.S. , Magajevski F.S. , Girio R.J.S. , Miashyro S. , Rodrigues L.H. , Scarcelli E.P. , Toma S.B.
Published: April 2022

RESUMO O objetivo desse trabalho foi verificar a ação de marcas diferentes de estreptomicinas polivalentes para o tratamento da leptospirose bovina em dose única (25 mg de estreptomicina por kg de peso corpóreo). O trabalho foi realizado com 14 touros adultos sorologicamente reagentes para Leptospira interrogans sorovar Hardjo, com título mínimo de 800 e com cultivo positivo. Os animais foram separados em 2 grupos de acordo com a marca da estreptomicina utilizada no tratamento, grupo 1: estreptomicina A e grupo 2: estreptomicina B. Os bovinos controles não receberam nenhum tratamento. Foram obtidas amostras de sangue e urina dos animais tratados e controles nos dias -1, 0, 1, 2, 3, 5, 10, 15 e 25; considerando-se o dia do tratamento como dia 0. Na urina dos bovinos tratados e controle foi realizada a reação em cadeia da polimerase (PCR) e isolamento com inoculação em hamsters. Observou-se que a estreptomicina da marca A, em dose única de 25 mg/kg de peso corpóreo, conseguiu eliminar a leptospirúria no grupo de touros após 24h do tratamento. Já no grupo de touros tratados com a estreptomicina da marca B, foi constatado a leptospirúria entre 48h e 72h, após o tratamento. Em ambos os grupos tratados, a resposta sorológica apresentou uma variação da queda dos níveis dos títulos de anticorpos aglutinantes, sendo que embora a estreptomicina A tenha aparentemente apresentado um melhor desempenho quando comparada com as médias geométricas dos títulos do grupo tratado com a estreptomicina B as médias não diferiram entre si pelo Teste de Tukey (P > 0,05). Nos bovinos tratados a leptospirúria foi intermitente e a média geométrica dos títulos foi menor que a média geométrica dos títulos dos bovinos controles. A diferença do efeito da ação das diferentes marcas de estreptomicina está possivelmente na qualidade da matéria prima produzida pelos laboratórios.
EFEITOS DA AFLATOXINA B 1 E FUMONISINA B1 SOBRE OS NÍVEIS SÉRICOS DE ASPARTATO AMINO-TRANSFERASE E PROTEÍNA TOTAL DE FRANGOS DE CORTE
Authors: Tessari E.N.C. , Oliveira C.A.F. , Cardoso A.L.S.P. , Ledoux D.R. , Rottinghaus G.E.
Published: April 2022

RESUMO O objetivo do presente trabalho foi avaliar os efeitos da aflatoxina B 1 (AFB1) e da fumonisina B1 (FB1) sobre os níveis séricos da enzima aspartato amino-transferase (AST) e de proteínas totais de frangos de corte alimentados com ração contendo as toxinas isoladas e em associação, nos níveis de 0, 50 e 200 ?g de AFB1/kg e 0, 50 e 200 mg de FB1/kg. O delineamento experimental foi o inteiramente casualizado em esquema fatorial 3 x 3, com 9 tratamentos e 12 repetições, totalizando 108 aves. As aves foram alimentadas com as rações contaminadas a partir do 8 o dia até o 41o dia de idade. Aos 41 dias de idade, houve um aumento (p < 0,05) nos níveis de AST das aves que receberam dietas contendo AFB1 e FB1, com exceção do grupo que recebeu somente 50 ?g de FB1/kg. Este aumento foi maior quanto mais elevados foram os níveis de AFB1, sendo que um efeito tóxico aditivo foi observado nos tratamentos de associação com 50 mg de AFB1/ kg e 50 ou 200 mg de FB1/kg. Aos 28 dias de idade, observou-se uma redução (p < 0,05) na concentração de proteína dos grupos que receberam ração com 200 ?g de AFB1/kg, com ou sem FB1, porém esta redução não foi observada aos 41 dias de idade. Conclui-se que a intoxicação de frangos de corte com AFB1 e FB1, isoladas ou associadas, causa um aumento na concentração sérica de AST e uma redução nos níveis de proteínas totais após 20 dias de exposição contínua através da ração.
FLUTUAÇÃO POPULACIONAL DE ALPHITOBIUS DIAPERINUS (PANZER, 1979) (COLEOPTERA: TENEBRIONIDAE), CAPTURADOS POR ARMADILHA DO TIPO SANDUÍCHE, EM GRANJA AVÍCOLA, NO MUNICÍPIO DE PELOTAS, RS
Authors: Pinto D.M. , Ribeiro P.B. , Bernardi E.
Published: April 2022

RESUMO Alphitobius diaperinus (Coleóptera: Tenebrionidae) coloniza substratos encontrados em granjas, criando-se em altas populações, causando problemas sanitários e econômicos. Para conhecer melhor o comportamento deste coleóptero avaliou-se a flutuação populacional em uma granja no Município de Pelotas, RS, durante 12 meses, através de armadilhas do tipo sanduíche. A. diaperinus ocorreu em todos os meses do período experimental, com uma densidade populacional que variou ao longo do ano, sendo capturados adultos e larvas.
NÍVEIS DE ADUBAÇÃO ORGÂNICA NA PRODUÇÃO DE CALÊNDULA E ARTRÓPODES ASSOCIADOS
Authors: Leite G.L.D. , Araújo C.B.O. , Amorim C.A.D. , Pêgo K.P. , Martins E.R. , Santos E.A.M.
Published: April 2022

RESUMO Avaliou-se o efeito de 4 níveis de adubação orgânica (0, 3, 6 e 9 kg/m2) na produção da calêndula (Calendula officinalis L.) e dos artrópodes associados em 2 cultivos no campus da Universidade Federal de Minas Gerais no Município de Montes Claros, MG. O delineamento experimental foi em blocos casualizados com 8 repetições. A dose de adubo orgânico, não residual, recomendada para produção quantitativa (número de capítulos/planta) e qualitativa (teor de flavonóides totais) de calêndula é de 6 kg/m2, contudo, deve-se atentar ao ataque de pulgões, já que esta dosagem o favorece, bem como de outros artrópodes, já que estes ocasionam perdas diretas e indiretas na produção de calêndula.
EFEITO DE FUNGICIDAS NO CONTROLE DA FERRUGEM DO FEIJOEIRO CAUSADA POR UROMYCES APPENDICULATUS EM CONDIÇÕES DE CAMPO
Authors: Modesto J.C. , Fenille R.C. , Habermann G.
Published: April 2022

RESUMO Avaliou-se a eficácia de fungicidas no controle da ferrugem do feijoeiro, em condições de campo. O experimento foi conduzido em Marília, SP, com plantas de feijão do grupo Carioca. Foram realizadas 3 pulverizações a cada 14 dias, utilizando-se 300 L de calda/ha, com os ingredientes ativos: trifloxystrobin + propiconazole (75 mL/ha), prothioconazole (100 mL ha-1); azoxystrobin (50 g ha-1) + nimbus 0,25% v/v e trifloxystrobin + tebuconazole (50 + 100, 60 + 120 e 75 + 150 mL ha-1). O controle proporcionado pelos ingredientes ativos foi avaliado nas folhas, sendo o índice de infecção calculado através de escala de notas dividida em nove níveis. Constatou-se q ue todos os fungicidas e doses utilizados foram eficientes no controle da ferrugem (Uromyces appendiculatus), proporcionando mais de 90% de eficiência. Na testemunha o índice de infecção chegou a 52% na terceira avaliação.
DETERMINAÇÃO EXPERIMENTAL DA DOSE DE REDUÇÃO DO CRESCIMENTO (GR50) E DA DOSE LETAL (LD50) DE SOJA IRRADIADA POR RAIOS GAMA
Authors: Barros A.C. de , Arthur V.
Published: April 2022

RESUMO O grau de radiosensibilidade de um embrião vegetal depende da espécie, do estágio de desenvolvimento deste embrião durante a irradiação, da dose empregada e do critério usado para medir o efeito biológico. Um dos critérios mais comuns utilizado para avaliar a radiosensibilidade de sementes é medir a altura da planta em um tempo determinado após a germinação. A dose de radiação necessária para provocar uma redução de 50% na altura média da população estudada chama-se GR50 (growth reduction to 50%) e a dose que mata 50% da população em estudo é chamada de dose letal para 50% da população ou LD50 (lethal dose to 50%). O objetivo deste trabalho foi estudar quantitativamente a sensibilidade da soja à radiação gama através do GR50 e LD50. As sementes variedade IAC 12 provenientes do Instituto Agronômico, Campinas, SP, foram irradiadas em uma fonte de 60Co tipo gammacell modelo 220 no CENA com taxa de dose de 1,481 kGy/h. As doses em kGy foram as seguintes: 0,0 (controle), 0,10, 0,20, 0,30, 0,40 e 0,50. Concluiu-se que o GR50 da soja ocorre em 0,30 ± 0,04 kGy. Não foi possível determinar o LD50 para a faixa de doses utilizadas neste experimento.
PARASITOS DE ANIMAIS SILVESTRES NO RIO GRANDE DO SUL: I-PIOLHOS DE ALGUNS ANSERIFORMES
Authors: Brum J.G.W. , Coimbra M.A. , Albano A.P. , Paulsen R.M.M.
Published: April 2022

RESUMO É reportado pela primeira vez no Estado do Rio Grande do Sul e no Brasil, o parasitismo dos piolhos menoponídeos Dicteisia tristis em tachã (Chaunatorquata), Holomenopon brevithoracicum em cisne-de-pescoço-preto (Cygnus melancoryphus) e Holomenopon leucoxanthum em marrecão da Patagônia (Netta peposaca), hospedeiros pertencentes à ordem Anseriformes.
Consumer-consumption characteristics of ready-to-eat sous vide food products within the habitual context of the household
Authors: Aviles María Victoria , Naef Elisa Fernanda , Gómez María Beatriz , Abalos Rosa Ana
Published: April 2022

Abstract Preparing cooked food by sous vide is an alternative for the design of products that permit the consumer to eat quickly and practically, without losing the sensory and nutritional characteristics of foods. This study aimed to determine the sensory properties, the overall liking, and the consumer-consumption characteristics and predispositions regarding ready-to-eat high-nutritional-quality preparations made with locally produced vegetables and cooked by the sous vide technique. A vegetable millefeuille and a chicken and vegetable hamburger were prepared for sensory characterization. Questions related to the consumer's consumption characteristics and predisposition to purchase this type of product were incorporated into the survey. Consumers performed the sensory evaluation in their home, within the habitual living ambience where they consumed their usual diet. The results revealed that most consumers accepted both millefeuille (100 %) and chicken and vegetable hamburger (92 %) and also reported a predisposition for purchasing both preparations (87 % and 84 %, respectively). The main characteristics that made the consumption attractive were quality (95%), market availability (89 %), lack of practical cooking skills (87 %), the price (84 %), the shortness of time invested in preparation (78 %), and the consumer's occupation (73 %). These types of preparations constitute an innovative alternative for the design of products of higher nutritional quality, without ignoring the requirements for their consumption.
CROSS-CULTURAL ADAPTATION AND VALIDATION OF THE CONSTIPATION SCORING SYSTEM FOR THE BRAZILIAN POPULATION
Authors: TANIGUCHI Thiago Masashi , ABREU Glicia Estevam de , PORTUGAL Matheus Mascarenhas , BARROSO JUNIOR Ubirajara
Published: April 2022

ABSTRACT Background Beyond Rome IV Criteria, the assessment of functional constipation in clinical practice can also be obtained by the Constipation Scoring System (CSS). By accessing the CSS, health professionals are able to measure this dysfunction, guiding initial therapeutic approach and post-treatment response. In addition, the CSS enables the standardization of results concerning functional constipation research. Objective To promote translation, cross-cultural adaptation and validation of the CSS for the Brazilian population. Methods To attain the score in Portuguese, the adaptation was accomplished in four steps (translation, back translation, application and adjustments). Afterward, the validation and adaptation to the Brazilian population was performed through test-retest. Results For adults, the convergent validity of the Brazilian version of the CSS showed a significant correlation to the Rome IV Criteria evinced by the positive Spearman correlation (r2) of 0.816 (P<0.001). Between the test-retest responses, the translated version of the score had a Cronbach’s Alpha of 0.972. A high level of internal consistency was also obtained when each item of the questionnaire was assessed separately, revealing an adequate internal reliability Conclusion The CSS was well adapted and accepted by the Brazilian population, demonstrating the linguistic and psychometric validity of this Portuguese version of the score.
EVALUATING THE IMPACT OF EARLY NUTRITIONAL ASSESSMENT AND INTERVENTION IN HOSPITALIZED LIVER CIRRHOSIS PATIENTS
Authors: JAVAID Nida , KHAN Zahra , ALI Muhammad Asif , TAHIR Sajid Khan
Published: April 2022

ABSTRACT Background Malnutrition is common in liver cirrhosis patients that is correlated with early complications, morbidity and mortality. Objective The purpose of the study was to assess nutritional status, impact of nutritional screening and intervention in liver cirrhosis patients by evaluating their actual energy and protein intake during hospital stay. Methods A cross sectional study was conducted wherein all patients’ nutritional status was defined by Subjective Global Assessment tool. Adequate energy and protein supply were planned and executed by using individualized nutritional plan for patients with dietitian’s collaboration. Anthropometric measurements included height, weight, body mass index, mid upper arm circumference, hand grip strength and triceps skin-fold thickness. Biochemical tests included haemoglobin, mean corpuscular haemoglobin, volume and concentration, albumin and liver function tests. To record the daily food intake, a 24-hour dietary recall was used. Results Overall 83 patients (mean age 55) were included, among them 46% of patients were moderately malnourished, 12% were normal, while 42% of cirrhotic patients were severely depleted according to Subjective Global Assessment. The mean intake of calories and protein was improved during stay in hospital after nutritional intervention and critical monitoring (P<0.05). Anthropometric measurements at baseline and discharge showed significant differences (P <0.05) in weight, body mass index, triceps skin fold thickness and mid upper arm circumference values, but not in hand grip strength that was associated with malnourishment among patients. Conclusion Providing individualized nutritional intervention and its monitoring by qualified dietitians during hospital stay helps to improve intake in patients that prevent further risk of malnutrition and related complications.
HEPATIC ALTERATIONS IN KIDNEY TRANSPLANT RECIPIENTS FROM THE LARGEST KIDNEY TRANSPLANT CENTER IN BRAZIL
Authors: VIEIRA Gustavo de Almeida , AMARAL Ana Cristina de Castro , CARVALHO FILHO Roberto José de , SOUZA Ana Lucia da Silva , MEDINA-PESTANA José Osmar , FERRAZ Maria Lucia Gomes
Published: April 2022

ABSTRACT Background Kidney transplant is the treatment of choice for patients with end-stage renal disease and is associated with lower mortality when compared to dialysis methods. Brazil is the country with the second largest number of kidney transplants in the world and among these patients it has been observed that liver abnormalities are common. The frequency of liver abnormalities ranges from 20-50% post-transplantation, and have an important impact on the survival and quality of life of these patients. There are scarce data about the frequency, causes and characteristics of these alterations. Objective To determine the prevalence of the different causes of hepatic abnormalities in kidney transplant recipients, to associate the characteristics of these abnormalities with demographic, epidemiological and clinical variables, to compare the characteristics of hepatic alterations between different etiologies, and to evaluate possible changes in diagnosis over two different periods of time. Methods Descriptive, cross-sectional observational, epidemiological study was conducted at the outpatient “Hepato-Rim”clinic of Hospital São Paulo (EPM/UNIFESP), a center providing specialized care for patients with hepatic abnormalities and underlying kidney diseases. Results Five-hundred eighty-one transplant patients were evaluated. The most prevalent etiologies of liver abnormalities were hepatitis C and B, iron overload, nonalcoholic fatty liver disease (NAFLD), and drug-induced liver injury (DILI). The most common cause — hepatitis C — was analyzed in greater detail. Compared to the other causes, this infection was more frequent in older patients, female patients, and patients with a longer time since transplantation and hemodialysis. Analysis of the two periods showed that patients of period 1 (P1 — 1993 to 2005) were older and were more frequently referred because of positive serology; referral due to aminotransferases abnormalities predominated during period 2 (P2 — 2006 to 2018). The predominant diagnoses were hepatitis C and B during P1 and NAFLD and DILI during P2. Conclusion Assessment of the main hepatic alterations in kidney transplant recipients is important because it permits better management of these patients in terms of diagnostic investigation and treatment and contributes to the prevention of complications in this special population.
FUNCTIONAL ABDOMINAL PAIN IS THE MAIN ETIOLOGY AMONG CHILDREN REFERRED TO TERTIARY CARE LEVEL FOR CHRONIC ABDOMINAL PAIN
Authors: MARTINS Gabriela Parússolo , SANDY Natascha Silva , ALVARENGA Lucas Rocha , LOMAZI Elizete Aparecida , BELLOMO-BRANDÃO Maria Angela
Published: April 2022

ABSTRACT Background Chronic abdominal pain (CAP) carries a significant burden of disease. The last edition of the Rome Criteria (Rome IV) allows the diagnosis of functional gastrointestinal disorders (FGIDs) according to symptoms-based criteria; however, patients continue to experience a delay in their diagnosis and to be submitted to different interventions before the establishment of a positive diagnosis. Objective We aimed to characterize etiology, clinical features, and interventions in a pediatric cohort of patients with CAP secondary to FGIDs, who were referred to our tertiary care university-affiliated hospital, in Brazil. Methods A retrospective descriptive study of children and adolescents (aged 20 years and younger) referred to our institution, from January/2013 to December/2018, for CAP, and who fulfilled criteria for FGIDs classified according to Rome IV criteria. Results Three hundred twenty-eight patients with CAP were screened, of which 67.9% (223 patients) fulfilled the criteria for FGIDs and were included in the study. Sixty percent were female, with a mean age of 8.3 years. At the time of referral, the mean duration of symptoms was 2.8 years. Length/height for age and weight for age mean z-scores were -0.08±1.87 and -0.38±1.62, respectively. Functional abdominal pain not otherwise specified was overall the most common diagnosis (70.4%). Before establishing the diagnosis of FGIDs, multiple pharmacological interventions were described, while after, the mainstay of therapy was education/reassurance and dietary interventions. Thirty-two percent of patients did not further require specialized follow-up. Conclusion Even at the tertiary care level, FGIDs were still the most common etiology of chronic abdominal pain, particularly functional abdominal pain not otherwise specified. Despite the relatively long duration of symptoms at referral, cessation of specialized care follow-up was possible in approximately a third of the cases.