bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparative Study on the Nutritional, Antioxidant, Thermal, Morphological and Diffraction Properties of Selected Cucurbit Seeds
Authors: Gade Sanghmitra R. , Meghwal Murlidhar , Prabhakar Pramod K. , Giuffrè Angelo Maria
Published: September 2022

Cucurbit seeds are highly nutritious but generally discarded as a waste by various processing sectors. This study aims to investigate the nutritional profile of seeds of ash gourd, pumpkin, watermelon and musk melon. The techniques, such as mineral, vitamin, amino acid and fatty acid profiling, will provide the nutritional status of the selected seed samples, while the antinutritional accounts will assess the safety of the food application. The thermal behavior of the seeds will guide the decision of the food application of the selected seeds. These seeds were found to be rich in protein and fat, minerals, vitamins and amino acids. The antinutritional compounds in the studied seeds were within the safety limits. Ash gourd seeds had the highest antioxidant activity at 85.11%, with the highest values of total phenols at 176.07 mg GAE/100 g and flavonoids at 159.16 mg QE/100 g. The peaks of the functional group at 3008 cm−1, 1651 cm−1, 1528 cm−1 and 1233 cm−1 denote the presence of polyunsaturated fatty acids, and amide I, II and III, respectively. The thermal analysis of the seeds reviled that the seeds were thermally stable and could be used in product development. The surface morphology was attributed to the interaction of fat, protein and carbohydrates. The semicrystalline or amorphous nature of the seeds resulted in an A-type pattern in the XRD analysis. The results obtained here supports the food application of the selected seeds.
Biochar-Induced Mitigation Potential of Greenhouse Gas Emissions Was Enhanced under High Soil Nitrogen Availability in Intensively-Irrigated Vegetable Cropping Systems
Authors: Zhang Yunfeng , Hwarari Delight , Yang Yuwen , Huo Ailing , Wang Jinyan , Yang Liming
Published: September 2022

Intensive irrigation coupled with excessive nitrogen (N) fertilizer input has resulted in high soil greenhouse gas (GHG) emissions in vegetable cropping systems. Biochar as a soil amendment has been advocated as a desirable option to reduce GHG emissions in agricultural systems, but its interactive effects with soil N availability in vegetable systems have yet to be clarified. We performed a field study to examine how biochar interacts with N fertilizer in driving annual methane (CH4) and nitrous oxide (N2O) emissions from an intensively-irrigated greenhouse vegetable cropping system acting as both sources of atmospheric CH4 and N2O in subtropical China. Biochar amendment significantly increased soil CH4 emissions by 33% and 85%, while it decreased soil N2O emissions by 22% and 12% with and without N fertilizer input, respectively. Fertilizer N combination weakened the positive response of CH4 to biochar while it enhanced the mitigation potential of biochar for N2O. Annual direct emission factors of fertilizer N for N2O were estimated to be 1.35% and 1.94% for the fields with and without biochar amendment, respectively. Annual flux-sustained global warming potential (SGWP) and greenhouse gas intensity (GHGI) were significantly decreased by biochar amendment, and this mitigation effect was enhanced with fertilizer N combination. Altogether, we highlight that biochar can reconcile higher yield and lower climatic impact in intensive vegetable cropping systems in subtropical China, particularly in vegetable soils with high N availability.
A Variant of the Deltoid Muscle and Its Clinical Implications: A Cadaveric Study
Authors: Gómez-Sánchez Stella Maris , Gómez-Esquer Francisco , Gil-Crujera Antonio , Palomar-Gallego Mª Angustias , Delcán-Giráldez José , Díaz-Gil Gema
Published: September 2022

Background: The deltoid is the muscle that forms the rounded contour of the shoulder. Anatomically, it seems to be made up of three different sets of fibers. The three anatomical portions in which the deltoid muscle are typically divided into the anterior (clavicular), the mean (acromial), and the posterior (spinal). Different variations of the deltoid muscle have been described in the literature. The analysis and knowledge of these anomalies are essential for surgeons and anatomists. Methods: A total of 21 specimens (12 women and 9 men) were used with a 10% formaldehyde solution. All specimens were dissected bilaterally (the classical dissection methodology was used). Results: In one corpse, an additional bilateral belly of the deltoid muscle was found. It was observed that this additional muscular belly was related to the fascial tissue of the deltoid muscle and its muscle fibers take origin from the middle third of the inferomedial bands of infraspinatus fascia. In addition, the belly attaches to the posterior belly of the deltoid muscle. The deltoid muscle and the additional belly were innervated by the axillar nerve, which is a lateral terminal bouquet of the posterior fascicle of the brachial plexus. Conclusions: This new variation of the deltoid muscle must be considered by surgeons, as well as abnormal conditions in terms of compartment syndrome involving the shoulder. Therefore, consideration of this variant is necessary for the correct diagnosis and treatment of trauma or other shoulder pathology that may be refractory to standard treatments.
Synergy between Mecillinam and Ceftazidime/Avibactam or Avibactam against Multi-Drug-Resistant Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae
Authors: List Karoline Knudsen , Kolpen Mette , Kragh Kasper Nørskov , Charbon Godefroid , Radmer Stine , Hansen Frank , Løbner-Olesen Anders , Frimodt-Møller Niels , Hertz Frederik Boetius
Published: September 2022

Background: Carbapenemase-producing Klebsiella pneumoniae and Escherichia coli have become a significant global health challenge. This has created an urgent need for new treatment modalities. We evaluated the efficacy of mecillinam in combination with either avibactam or ceftazidime/avibactam against carbapenemase-producing clinical isolates. Materials and methods: Nineteen MDR clinical isolates of K. pneumoniae and E. coli were selected for the presence of blaKPC, blaNDM, blaOXA or blaIMP based on whole-genome sequencing and phenotypic susceptibility testing. We tested the synergy between mecillinam and avibactam or ceftazidime/avibactam. We used time–kill studies in vitro and a mouse peritonitis/sepsis model to confirm the synergistic effect. We investigated avibactam’s impact on mecillinam´s affinity for penicillin-binding proteins with a Bocillin assay, and cell changes with phase-contrast and confocal laser scanning microscopy. Results: Mecillinam combined with ceftazidime/avibactam or avibactam substantially reduced MICs (from up to >256 µg/mL to <0.0016 µg/mL) for 17/18 strains. Significant log-CFU reductions were confirmed in time–kill and in vivo experiments. The Bocillin assay did not reveal changes. Conclusion: Mecillinam in combination with avibactam or ceftazidime/avibactam has a notable effect on most types of CPEs, both in vitro and in vivo. The mecillinam/avibactam combination treatment could be a new efficient antibiotic treatment against multi-drug-resistant carbapenemase-producing Gram-negative pathogens.
Phytochemical Composition and Biological Activities of Arctium minus (Hill) Bernh.: A Potential Candidate as Antioxidant, Enzyme Inhibitor, and Cytotoxic Agent
Authors: İlgün Selen , Karatoprak Gökçe Şeker , Polat Derya Çiçek , Şafak Esra Köngül , Yıldız Gülsüm , Küpeli Akkol Esra , Sobarzo-Sánchez Eduardo
Published: September 2022

Arctium minus (Hill) Bernh. (Asteraceae), which has a wide distribution area in Turkey, is a medicinally important plant. Eighty percent methanol extracts of the leaf, flower head, and root parts of A. minus were prepared and their sub-fractions were obtained. Spectrophotometric and chromatographic (high-performance liquid chromatography) techniques were used to assess the phytochemical composition. The extracts were evaluated for antioxidant activity by diphenyl-2-picrylhydrazil radical (DPPH●), 2,2′-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS●+) radical scavenging, and β-carotene linoleic acid bleaching assays. Furthermore, the extracts were subjected to α-amylase, α-glucosidase, lipoxygenase, and tyrosinase enzyme inhibition tests. The cytotoxic effects of extracts were investigated on MCF-7 and MDA-MB-231 breast cancer cell lines. The richest extract in terms of phenolic compounds was identified as the ethyl acetate sub-fraction of the root extract (364.37 ± 7.18 mgGAE/gextact). Furthermore, chlorogenic acid (8.855 ± 0.175%) and rutin (8.359 ± 0.125%) were identified as the primary components in the leaves’ ethyl acetate sub-fraction. According to all methods, it was observed that the extracts with the highest antioxidant activity were the flower and leaf ethyl acetate fractions. Additionally, ABTS radical scavenging activity of roots’ ethyl acetate sub-fraction (2.51 ± 0.09 mmol/L Trolox) was observed to be as effective as that of flower and leaf ethyl acetate fractions at 0.5 mg/mL. In the β-carotene linoleic acid bleaching assay, leaves’ methanol extract showed the highest antioxidant capacity (1422.47 ± 76.85) at 30 min. The enzyme activity data showed that α-glucosidase enzyme inhibition of leaf dichloromethane extract was moderately high, with an 87.12 ± 8.06% inhibition value. Lipoxygenase enzyme inhibition was weakly detected in all sub-fractions. Leaf methanol extract, leaf butanol, and root ethyl acetate sub-fractions showed 99% tyrosinase enzyme inhibition. Finally, it was discovered that dichloromethane extracts of leaves, roots, and flowers had high cytotoxic effects on the MDA-MB-231 cell line, with IC50 values of 21.39 ± 2.43, 13.41 ± 2.37, and 10.80 ± 1.26 µg/mL, respectively. The evaluation of the plant extracts in terms of several bioactivity tests revealed extremely positive outcomes. The data of this study, in which all parts of the plant were investigated in detail for the first time, offer promising results for future research.
Rosa davurica Pall., Rosa rugosa Thumb., and Rosa acicularis Lindl. Originating from Far Eastern Russia: Screening of 146 Chemical Constituents in Three Species of the Genus Rosa
Authors: Razgonova Mayya P. , Bazhenova Bayana A. , Zabalueva Yulia Yu. , Burkhanova Anastasia G. , Zakharenko Alexander M. , Kupriyanov Andrey N. , Sabitov Andrey S. , Ercisli Sezai , Golokhvast Kirill S.
Published: September 2022

Rosa rugosa Thumb., Rosa davurica Pall., and Rosa acicularis Lindl. contain a large number of target analytes which are bioactive compounds. High performance liquid chromatography (HPLC), in combination with the ion trap (tandem mass spectrometry), was used to identify target analytes in MeOH extracts of R. rugosa, R. davurica, and R. acicularis, originating from the Russian Far East, Trans-Baikal Region, and Western Siberia. The results of initial studies revealed the presence of 146 compounds, of which 115 were identified for the first time in the genus Rosa (family Rosaceae). The newly identified metabolites belonged to 18 classes, including 14 phenolic acids and their conjugates, 18 flavones, 7 flavonols, 2 flavan-3-ols, 2 flavanones, 3 stilbenes, 2 coumarins, 2 lignans, 9 anthocyanins, 3 tannins, 8 terpenoids, 3 sceletium alkaloids, 4 fatty acids, 2 sterols, 2 carotenoids, 3 oxylipins, 3 amino acids, 5 carboxylic acids, etc. The proven richness of the bioactive components of targeted extracts of R. rugosa, R. davurica, and R. acicularis invites extensive biotechnological and pharmaceutical research, which can make a significant contribution both in the field of functional and enriched nutrition, and in the field of cosmetology and pharmacy.
Long Short-Term Memory (LSTM)-Based Dog Activity Detection Using Accelerometer and Gyroscope
Authors: Hussain Ali , Begum Khadija , Armand Tagne Poupi Theodore , Mozumder Md Ariful Islam , Ali Sikandar , Kim Hee Cheol , Joo Moon-Il
Published: September 2022

Dog owners are extremely driven to comprehend the activity and health of their dogs and to keep tabs on their well-being. Dogs’ health and well-being, whether as household pets or service animals, are critical issues that are addressed seriously for moral, psychological, and economical reasons. Evaluations of a dog’s welfare depend on quantitative assessments of the frequency and variability of certain behavioral features, which are sometimes challenging to make in a dog’s normal environment. While it is challenging to obtain dogs’ behavioral patterns, it is nearly impossible to directly identify one distinct behavior when they are roaming around at will. Applications for automatic pet monitoring include real-time surveillance and monitoring systems that accurately identify pets using the most recent methods for the classification of pet activities. The suggested method makes use of a long short-term memory (LSTM)-based method to detect and classify the activities of dogs based on sensor data (i.e., accelerometer and gyroscope). The goal of this study is to use wearable sensor data and examine the activities of dogs using recurrent neural network (RNN) technology. We considered 10 pet behaviors, which include walking, sitting, down, staying, feeding, sideways, leaping, running, shaking, and nose work. As dog activity has a wider diversity, experimental work is performed on the multi-layer LSTM framework to have a positive influence on performance. In this study, data were collected from 10 dogs of various ages, sexes, breeds, and sizes in a safe setting. Data preprocessing and data synchronization were performed after the collection of data. The LSTM model was trained using the preprocessed data and the model’s performance was evaluated by the test dataset. The model showed good accuracy and high performance for the detection of 10 activities of dogs. This model will be helpful for the real-time monitoring of dogs’ activity, thus improving the well-being of dogs.
The Impact of Traditional Raw Earth Dwellings’ Envelope Retrofitting on Energy Saving: A Case Study from Zhushan Village, in West of Hunan, China
Authors: Xie Liang , Li Zhe , Li Jiayu , Yang Guanglei , Jiang Jishui , Liu Zhezheng , Tong Shuyuan
Published: September 2022

This study presents the CO2 emissions and energy performance of traditional raw earth dwellings’ envelope retrofitting located in the Zhushan Village, western Hunan Province, China. The numerical simulations of heating energy consumption on the building models were performed using DesignBuilder, an energy simulation program. The energy performance was evaluated using the indexes (including energy consumption, CO2 emissions, heat balance analysis, and air temperature profiles). The detailed evaluation process of the energy performance is presented as follows. First, the current situation was analyzed through the field research, and two typical building models were built. Second, all schemes were simulated using the DesignBuilder software. Subsequently, the four main retrofit measures (replacing the external insulation windows, setting the external wall insulation layer, setting the roof insulation layer, and setting the ceiling insulation layer) were analyzed, respectively. The optimal parameters of the respective retrofit measure were calculated. Lastly, a multi-objective optimization analysis was conducted on all retrofit plans using the coupling method. In the winter, the results indicated that the “I-shape” dwelling heat consumption of the enclosure structure was reduced by 12.8 kW·h/m2, and the CO2 emissions were reduced by 882.8 kg. While in the benchmark building, the results showed that the “L-shape” dwelling heat consumption of the enclosure structure was decreased by 13.27 kW·h/m2, and the CO2 emissions were reduced by 894.4 kg. As the renewal scheme has been progressively implemented, the whole Zhushan Village will save energy by 11.2 × 104 kW·h after the insulation renewal of the envelope structure is completed.
Multispecies Assessment of Anthropogenic Particle Ingestion in a Marine Protected Area
Authors: Compa Montserrat , Alomar Carme , López Cortès María , Rios-Fuster Beatriz , Morató Mercè , Capó Xavier , Fagiano Valentina , Deudero Salud
Published: September 2022

We have applied a multispecies ecosystem approach to analyse the ingestion of anthropogenic particles (AP) in the gastrointestinal tract of 313 individuals (17 fish species and 8 invertebrate species) from pelagic, demersal and benthic habitats in a marine protected area off the Western Mediterranean (Cabrera National Park). We have quantified and characterized the ingestion at several taxonomic levels of fish, sea urchins, sea cucumbers, bivalves, and jellyfish in relation to biotic/abiotic factors based on taxonomic groups, trophic guilds (functional groups) and habitats. AP ingestion occurrence ranged from 26 to 100% with no significant differences among taxonomic groups. The fish within the MPA showed an overall ingestion occurrence ranging from 0 to 100%, the echinoderms from 29 to 100%, the bivalves from 72 to 96% and the jellyfish 36% ingestion. The ecosystem approach applied to evaluate overall AP ingestion within the species reported that for trophic guilds, the omnivorous species ingested the highest amounts of anthropogenic items, while herbivores ingested significantly fewer items than all other trophic guilds. Moreover, no significant differences were found amongst habitats, indicating a homogeneous spatial distribution of APs at all studied habitats. The multispecies approach provided insight into the high APs exposure to species within Cabrera MPA, highlighting the potential harm linked with marine litter that threatens marine biodiversity.
Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo
Authors: Papoutsopoulou Stamatia , Pollock Liam , Williams Jonathan M. , Abdul-Mahdi Maya M. L. F. , Dobbash Reyhaneh , Duckworth Carrie A. , Campbell Barry J.
Published: September 2022

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has a major protective role against intestinal inflammation. We recently revealed that intestinal epithelial cells in vitro regulate NFκB-driven transcriptional responses to TNF via an autocrine mechanism dependent on IL-10 secretion. Here in this study, we investigated the impact of IL-10 deficiency on the NFκB pathway and its downstream targets in the small intestinal mucosa in vivo. We observed dysregulation of TNF, IκBα, and A20 gene and protein expression in the small intestine of steady-state or TNF-injected Il10−/− mice, compared to wild-type C57BL6/J counterparts. Upon TNF injection, tissue from the small intestine showed upregulation of NFκB p65[RelA] activity, which was totally diminished in Il10−/− mice and correlated with reduced levels of TNF, IκBα, and A20 expression. In serum, whilst IgA levels were noted to be markedly downregulated in IL-10-deficient- mice, normal levels of mucosal IgA were seen in intestine mucosa. Importantly, dysregulated cytokine/chemokine levels were observed in both serum and intestinal tissue lysates from naïve, as well as TNF-injected Il10−/− mice. These data further support the importance of the IL-10-canonical NFκB signaling pathway axis in regulating intestinal mucosa homeostasis and response to inflammatory triggers in vivo.
Spectral Properties Echoing the Tautomerism of Milrinone and Its Application to Fe3+ Ion Sensing and Protein Staining
Authors: Zhu Hanming , Ma Pan , Qian You , Xia Jiaoyun , Gong Fuchun , Chen Lusen , Xu Lujie
Published: September 2022

Knowledge on the spectral properties of the tautomers of milrinone (MLR) in solvents and solid-state, as well as under light conditions is of critical importance from both theoretical and practical points of view. Herein, we investigated the spectral properties of MLR in different conditions using UV-Vis and fluorescence spectroscopies. The experimental results demonstrated that MLR can undergo the tautomerization reaction induced by solvent polarity, light and pH, eliciting four tautomeric structures (enol, keto, anion, and cation forms). The interesting multi-functional groups in MLR enable it to coordinate with metal ions or to recognize gust molecules by H-bonding. In the use of MLR as an excited-state intermolecular proton transfer (inter-ESPT) fluorescent probe, a highly sensitive and selective analysis of Fe3+ was developed, which offered a sensitive detection of Fe3+ with the detection limit of 3.5 nM. More importantly, MLR exhibited the ability of anchoring proteins and led to the recognition-driven turn-on inter-ESPT process, highlighting the potential for the probe to image proteins in electrophoresis gels. The spectral experimental results revealed the possible degradation mechanism, so that we can better understand the side effects of oral preparations. The use of the available drug as an inter-ESPT fluorescent probe is simple and accurate, providing a good method for Fe3+ ion sensing and protein staining.
Tandem Walk in Simulated Martian Gravity and Visual Environment
Authors: Rosenberg Marissa J. , Koslovsky Matthew , Noyes Matthew , Reschke Millard F. , Clément Gilles
Published: September 2022

Astronauts returning from long-duration spaceflights experience visual-vestibular conflicts that causes motion sickness, perceptions that the environment is moving when it is not, and problems with walking and other functional tasks. To evaluate whether astronauts will have similar decrements after they land on Mars following exposure to weightlessness, participants were held by a device that offloads their weight, first entirely (0 G), and then partially (0.38 G) or not at all (1 G). Tandem (heel-to-toe) walk on a medium-density foam surface was used to assess the subject’s walking performance. Two visual conditions in virtual reality were investigated: normal vision and a visual-vestibular conflict generated by disorienting optokinetic stimulation (DOS). Tandem walking performance with DOS was better in 0.38 G compared to 1 G. Tandem walking performance in DOS in 1 G was not significantly different from tandem walking performance after spaceflight or bed rest. The increased tandem walking performance in 0.38 G compared to 1 G was presumably due to an increased cone of stability, allowing a larger amplitude of body sway without resulting in a fall. Tandem walking on a compliant foam surface with a visual-vestibular conflict is a potential analog for simulating postflight dynamic balance deficits in astronauts.
Volitional Generation of Reproducible, Efficient Temporal Patterns
Authors: Ning Yuxiao , Wan Guihua , Liu Tengjun , Zhang Shaomin
Published: September 2022

One of the extraordinary characteristics of the biological brain is the low energy expense it requires to implement a variety of biological functions and intelligence as compared to the modern artificial intelligence (AI). Spike-based energy-efficient temporal codes have long been suggested as a contributor for the brain to run on low energy expense. Despite this code having been largely reported in the sensory cortex, whether this code can be implemented in other brain areas to serve broader functions and how it evolves throughout learning have remained unaddressed. In this study, we designed a novel brain–machine interface (BMI) paradigm. Two macaques could volitionally generate reproducible energy-efficient temporal patterns in the primary motor cortex (M1) by learning the BMI paradigm. Moreover, most neurons that were not directly assigned to control the BMI did not boost their excitability, and they demonstrated an overall energy-efficient manner in performing the task. Over the course of learning, we found that the firing rates and temporal precision of selected neurons co-evolved to generate the energy-efficient temporal patterns, suggesting that a cohesive rather than dissociable processing underlies the refinement of energy-efficient temporal patterns.
Study on Influence of Numerical Simulation Accuracy on High Core Wall Rockfill Dam Deformation and Crack Analysis
Authors: Zhou Xiongxiong , Wu Bo , Li Li
Published: September 2022

With the increasing height of rockfill dams, detailed and effective numerical simulation for the dam design and construction has become increasingly important. This paper develops a detailed and effective finite element simulation program for the construction and impoundment process of high large-scale earth rockfill dams and studies the influence of numerical simulation accuracy on dam deformation and crack analysis. By establishing the subdivision mode of conventional elements, the mesh generation of detailed finite element simulation is realized. Using the row compression storage method and the MPI parallel method, the calculation program of the total stiffness matrix is developed on the Linux operating system. On the basis of the detailed simulation program above, the filling and impoundment process of Pubugou high core wall rockfill dam is simulated, and it is found that the influence of simulation accuracy on the core wall zone is greater than that on the rockfill zone. Compared with the dam construction process, the detailed simulation of the impoundment process is more necessary. When simulating the impoundment process, there is a significant effect of simulation accuracy on the simulation result of the upstream side of the core wall and the upstream filtration zone. So, it is necessary to simulate the impoundment process of the earth rockfill dam in detail. Moreover, using the detailed method to simulate the impoundment process of earth rockfill dams can more accurately predict dam cracks.
Proteasome Inhibitors and Their Potential Applicability in Osteosarcoma Treatment
Authors: Van Stiphout Cassidy M. , Luu Anita K. , Viloria-Petit Alicia M.
Published: September 2022

Osteosarcoma (OS) is the most common type of bone cancer, with ~30% of patients developing secondary/metastatic tumors. The molecular complexity of tumor metastasis and the lack of effective therapies for OS has cultivated interest in exploiting the proteasome as a molecular target for anti-cancer therapy. As our understanding towards the behavior of malignant cells expands, it is evident that cancerous cells display a greater reliance on the proteasome to maintain homeostasis and sustain efficient biological activities. This led to the development and approval of first- and second-generation proteasome inhibitors (PIs), which have improved outcomes for patients with multiple myeloma and mantle cell lymphoma. Researchers have since postulated the therapeutic potential of PIs for the treatment of OS. As such, this review aims to summarize the biological effects and latest findings from clinical trials investigating PI-based treatments for OS. Integrating PIs into current treatment regimens may better outcomes for patients diagnosed with OS.
Update in Molecular Testing for Intraocular Lymphoma
Authors: Heiferman Michael J. , Yu Michael D. , Mruthyunjaya Prithvi
Published: September 2022

The diagnosis of primary vitreoretinal lymphoma and central nervous system lymphoma is challenging. In cases with intraocular involvement, vitreous biopsy plays a pivotal role. Several diagnostic tests are employed to confirm a diagnosis and include cytologic evaluation, immunohistochemistry, flow cytometry, and cytokine analysis. The limitations of these conventional diagnostic tests stem from the often paucicellular nature of vitreous biopsy specimens and the fragility of malignant cells ex vivo. Several emerging molecular techniques show promise in improving the diagnostic yield of intraocular biopsy, possibly enabling more accurate and timely diagnoses. This article will review existing diagnostic modalities for intraocular lymphoma, with an emphasis on currently available molecular tests.
Long-Term Management of Advanced Basal Cell Carcinoma: Current Challenges and Future Perspectives
Authors: Heppt Markus V. , Gebhardt Christoffer , Hassel Jessica C. , Alter Mareike , Gutzmer Ralf , Leiter Ulrike , Berking Carola
Published: September 2022

The first-line therapy for locally advanced basal cell carcinoma (laBCC) is Hedgehog pathway inhibitors (HHIs), as they achieve good efficacy and duration of response. However, toxicity in the course of long-term treatment may lead to a decrease in the quality of life, and consequently to interruption or even discontinuation of therapy. As HHI therapy is a balancing act between effectiveness, adverse events, quality of life, and adherence, numerous successful treatment strategies have evolved, such as dose reduction and dose interruptions with on-off treatment schedules or interruptions with re-challenge after progression. As a small percentage of patients show primary or acquired resistance to HHIs, the inhibition of programmed cell death protein 1 (PD-1) has been approved as a second-line therapy, which may also be accompanied by immune-related toxicities and non-response. Thus, optimization of current treatment schedules, novel agents, and combination strategies are urgently needed for laBCC. Here, we narratively model the treatment sequence for patients with laBCC and summarize the current state of approved treatment regimens and therapeutic strategies to optimize the long-term management of laBCC.
A Novel tiRNA-Gly-GCC-1 Promotes Progression of Urothelial Bladder Carcinoma and Directly Targets TLR4
Authors: Qin Chuan , Chen Zheng-Hao , Cao Rui , Shi Ming-Jun , Tian Ye
Published: September 2022

Background: Patients with urothelial bladder carcinoma (UBC) have a poor prognosis and a high risk of progression. Recently, tRNA-derived small RNAs (tsRNAs), a novel type of noncoding RNA, have been identified. In our previous study, we found tiRNA-Gly-GCC-1 was significantly upregulated in UBC tissue and might target the predicted target gene toll-like receptor 4 (TLR4) to play a regulatory role in UBC. Thus, the aim of this study was to identify the functional roles of tiRNA-Gly-GCC-1 and the relationship between tiRNA-Gly-GCC-1 and TLR4. Methods: After lentiviral transfection in 5637 and T24 cell lines, quantitative reverse transcription-PCR, Cell Counting Kit-8, IncuCyte ZOOM™ live cell imaging, flow cytometry, Transwell assays, scratch assay, and luciferase assay were performed. Results: The results showed down-regulation of tiRNA-Gly-GCC-1 inhibits cell proliferation, migration and invasion, promotes cell apoptosis, and affects the cell cycle. Besides, tiRNA-Gly-GCC-1 was found to inhibit TLR4 expression by directly targeting its 3′UTR. Conclusions: Our study demonstrated that tiRNA-Gly-GCC-1 promotes the progression of UBC and directly targets TLR4. This study provides novel insights for future investigations to explore the mechanisms and therapeutic targets for UBC.
The Feasibility of Stereotactic Body Proton Beam Therapy for Pancreatic Cancer
Authors: Shin Hyunju , Yu Jeong Il , Park Hee Chul , Yoo Gyu Sang , Cho Sungkoo , Park Joon Oh , Lee Kyu Taek , Lee Kwang Hyuck , Lee Jong Kyun , Park Joo Kyung , Heo Jin Seok , Han In Woong , Shin Sang Hyun
Published: September 2022

Background/Purpose: This study aimed to evaluate the clinical outcomes of stereotactic body proton beam therapy (SBPT) for pancreatic cancer. Methods: This retrospective study included 49 patients who underwent SBPT for pancreatic cancer between 2017 and 2020. Survival outcomes, bowel-related toxicities, and failure patterns were analysed. SBPT was performed after induction chemotherapy in 44 (89.8%) patients. The dose-fractionation scheme included 60 gray (Gy) relative biological effectiveness (RBE) in five fractions (n = 42, 85.7%) and 50 GyRBE in five fractions (n = 7, 14.3%). The median follow-up was 16.3 months (range, 1.8–45.0 months). Results: During follow-up, the best responses were complete response, partial response, and stable disease in four (8.2%), 13 (26.5%), and 31 (63.3%) patients, respectively. The 2-year overall survival, progression-free survival, and local control (LC) rates were 67.6%, 38.0%, and 73.0%, respectively. Grade ≥ 3 gastroduodenal (GD) toxicity occurred in three (6.1%) patients. Among them, one patient underwent endoscopic haemostasis. The other two patients received surgical management. They were followed up without disease progression for >30 months after SBPT. Overall, there was no significant dosimetric difference between the grade ≥ 2 and lower toxicity groups. Conclusions: SBPT provides relatively high LC rates with acceptable toxicities in pancreatic cancer.
Engraftment of Allotransplanted Tumor Cells in Adult rag2 Mutant Xenopus tropicalis
Authors: Tulkens Dieter , Dimitrakopoulou Dionysia , Boelens Marthe , Van Nieuwenhuysen Tom , Demuynck Suzan , Toussaint Wendy , Creytens David , Van Vlierberghe Pieter , Vleminckx Kris
Published: September 2022

Modeling human genetic diseases and cancer in lab animals has been greatly aided by the emergence of genetic engineering tools such as TALENs and CRISPR/Cas9. We have previously demonstrated the ease with which genetically engineered Xenopus models (GEXM) can be generated via injection of early embryos with Cas9 recombinant protein loaded with sgRNAs targeting single or multiple tumor suppressor genes. What has been lacking so far is the possibility to propagate and characterize the induced cancers via transplantation. Here, we describe the generation of a rag2 knockout line in Xenopus tropicalis that is deficient in functional T and B cells. This line was validated by means of allografting experiments with primary tp53−/− and apc+/−/tp53−/− donor tumors. In addition, we optimized available protocols for the sub-lethal irradiation of wild-type X. tropicalis froglets. Irradiated animals also allowed the stable, albeit transient, engraftment of transplanted X. tropicalis tumor cells. The novel rag2−/− line and the irradiated wild-type froglets will further expand the experimental toolbox in the diploid amphibian X. tropicalis and help to establish it as a versatile and relevant model for exploring human cancer.
Evaluation of Blood Soluble CD26 as a Complementary Biomarker for Colorectal Cancer Screening Programs
Authors: De Chiara Loretta , Barcia-Castro Leticia , Gallardo-Gómez María , Páez de la Cadena María , Martínez-Zorzano Vicenta S. , Rodríguez-Berrocal Francisco J. , Bujanda Luis , Etxart Ane , Castells Antoni , Balaguer Francesc , Jover Rodrigo , Cubiella Joaquín , Cordero Oscar J.
Published: September 2022

Fecal hemoglobin immunodetection (FIT) in combination with endoscopy has been implemented to reduce mortality from colorectal cancer (CRC), although there are issues that can be improved in relation to participation rates. We studied whether the blood biomarker soluble-CD26 (sCD26), related at least in part to the immune system and inflammation, and/or its dipeptidyl peptidase enzyme activity (DPP4), could help reduce false positives. In a cohort of 1703 individuals who underwent colonoscopy and had a serum sample, sCD26 and DPP4 activity showed statistically significant differences regarding sex and age. According to the colonoscopy findings, sCD26 and DPP4 activity progressively decreased in advanced adenomas and CRC, with statistically significant differences, even between both groups; 918 of them had a FIT result (n = 596 positive cases) with approximately 70% of these (n = 412) false positives. With cut-offs of 440 ng/mL for sCD26, 42 mU/mL for DPP4, and 11 ng/mU for their ratio, the combined information of the three biomarkers (at least positive for one biomarker) identified almost all advanced adenomas and CRC cases in the FIT cohort with approximately half of the false positives compared to FIT. A sequential testing strategy with FIT and our blood biomarker test is proposed.
Insight into LncRNA- and CircRNA-Mediated CeRNAs: Regulatory Network and Implications in Nasopharyngeal Carcinoma—A Narrative Literature Review
Authors: Zhang Senmiao , Li Yanling , Xin Shuyu , Yang Li , Jiang Mingjuan , Xin Yujie , Wang Yiwei , Yang Jing , Lu Jianhong
Published: September 2022

Nasopharyngeal carcinoma (NPC) is a kind of head-and-neck malignant tumor, and distant metastasis treatment resistance is the leading cause of patient death. In-depth understanding of NPC progression and treatment failure remains to be explored. Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) are noncoding RNAs that play key regulatory role in shaping tumor cell activities. Recent studies have revealed that lncRNA and circRNA function as competitive endogenous RNAs (ceRNAs) by regulating the posttranscriptional expression of genes as miRNA baits. The imbalanced ceRNA networks derived from lncRNA/circRNA-miRNA-mRNA interaction are widely found to contribute to NPC development. Herein, we summarize typical examples of lncRNA/circRNA-associated ceRNAs in recent years, which involved the potential molecular mechanisms in the regulation of proliferation, apoptosis, treatment resistance and metastasis of NPC, and discuss their potential clinical significance in the prognosis and treatment of NPC. Interpreting the involvement of ceRNAs networks will provide new insight into the pathogenesis and treatment strategies of NPC. However, ceRNA regulatory mechanism has some limitations currently. Screening the most effective ceRNA targets and the clinical application of ceRNA still has many challenges.
Pd-Catalyzed Hirao P–C Coupling Reactions with Dihalogenobenzenes without the Usual P-Ligands under MW Conditions
Authors: Huszár Bianka , Varga Petra Regina , Szűcs Nóra Á. , Simon András , Drahos László , Keglevich György
Published: September 2022

A literature survey of the P–C coupling reactions of 1,4-and 1,2-bromo-iodobenzenes with diphenylphosphine oxide or diethyl phosphite under different conditions comprising Pd-, Ni-, or Cu-catalysis revealed that, depending on the experimental details, the yields of the corresponding >P(O)-bromobenzenes were rather diverse and occasionally contradicting. Therefore, the reactivity of a series of 1,4-, 1,3- and 1,2-dibromo- and bromo-iodobenzenes with the above mentioned P-reagents was evaluated under the “P-ligand-free” microwave (MW)-assisted conditions elaborated by us. Starting from dibromobenzenes and iodo-bromoarenes, practical and competent syntheses were developed for phosphonoyl- and phosphinoyl-bromoarenes, and, in a few instances, for arenes with two P-functions. The cheaper dibromobenzenes may be substituted for the bromo-iodo derivatives. In all, 12 products were prepared in yields of 45–82%. They were fully characterized. The method described does not require the use of traditional P-ligands.
The C-H Bond Activation Triggered by Subsurface Mo Dopant on MgO Catalyst in Oxidative Coupling of Methane
Authors: Sun Xiaoying , Li Xinyu , Liu Yue , Yu Zhan , Li Bo , Zhao Zhen
Published: September 2022

In this work, density functional theory calculations are performed to explore the unique role of Mo dopant on MgO in oxidative coupling of methane. It is revealed that subsurface Mo dopant significantly enhanced the adsorption and activation of oxygen molecules. The combination of adsorbed oxygen and surface Mg exhibited a balanced activity for C-H bond activation and release of methyl radical which paves the way to activate methane with a promising yield.
Recent Developments in Activated Carbon Catalysts Based on Pore Size Regulation in the Application of Catalytic Ozonation
Authors: Yang Jin , Fu Liya , Wu Fachao , Chen Xingxing , Wu Changyong , Wang Qibao
Published: September 2022

Due to its highly developed pore structure and large specific surface area, activated carbon is often used as a catalyst or catalyst carrier in catalytic ozonation. Although the pore structure of activated carbon plays a significant role in the treatment of wastewater and the mass transfer of ozone molecules, the effect is complicated and unclear. Because different application scenarios require catalysts with different pore structures, catalysts with appropriate pore structure characteristics should be developed. In this review, we systematically summarized the current adjustment methods for the pore structure of activated carbon, including raw material, carbonization, activation, modification, and loading. Then, based on the brief introduction of the application of activated carbon in catalytic ozonation, the effects of pore structure on catalytic ozonation and mass transfer are reviewed. Furthermore, we proposed that the effect of pore structure is mainly to provide catalytic active sites, promote free radical generation, and reduce mass transfer resistance. Therefore, large external surface area and reasonable pore size distribution are conducive to catalytic ozonation and mass transfer.
The Inhibitive Effect of Sebacate-Modified LDH on Concrete Steel Reinforcement Corrosion
Authors: Caballero David , Beltrán-Cobos Ruben , Tavares Fabiano , Cruz-Yusta Manuel , Granados Luis Sánchez , Sánchez-Moreno Mercedes , Pavlovic Ivana
Published: September 2022

In recent decades, layered double hydroxides (LDH) have been proposed as innovative corrosion inhibitors for reinforced concrete. Their protective action is based on the ability to intercalate specific anions in the interlayer and on their ability to exchange the intercalated anion. In the present study, an organically charged LDH, with sebacate anions in the interlayer (LDH-S), is proposed as a water-repellent additive for mortar. The waterproofing efficiency of LDH-S and the associated corrosion inhibition ability has been evaluated in reinforced mortar samples. A 42% decrease in the water capillary absorption coefficient has been estimated when 3% LHD-S is added to a mortar. Both the passivation processes of the steel rebars during the curing period and the initiation of corrosion due to chloride exposure have been studied by electrochemical measurements. Three different mortars have been evaluated: reference mortar (REF), mortar with Mg-Al LDH (LDH), and mortar with LDH-sebacate (LDH-S). The latter has shown an important protective capacity for preventing the initiation of corrosion by chloride penetration, with an inhibitory efficiency of 74%. The presence of LDHs without sebacate in the interlayer also improved the performance of the mortar against rebar corrosion, but with lower efficiency (23% inhibitory efficiency). However, this protection is lost after continued chloride exposure over time, and corrosion initiates similarly to the reference mortar. The low corrosion current density values registered when LDH-S is added to the mortar may be related to the increased electrical resistance recorded in this mortar.
Impact of Diabetes on Short-Term and Long-Term Outcomes of Ampullary Adenocarcinoma Patients after Curative Pancreatoduodenectomy
Authors: Zhang Xiaojie , Sun Chongyuan , Fei He , Li Zefeng , Guo Chunguang , Chen Yingtai , Che Xu , Zhao Dongbing
Published: September 2022

Background: Many studies have confirmed that diabetes was associated with prognosis in many malignant cancer types. However, the impact of diabetes on ampullary carcinoma (AC) has not been investigated. Methods: A total of 266 AC patients in the National Cancer Center of China between January 1998 and December 2020 were retrospectively reviewed. The postoperative complication rate, postoperative recurrence rate, and long-term survival were compared between the diabetes group and the no diabetes group. Results: A total of 32 AC patients (12.03%) were diagnosed with diabetes before surgery. In total, 111 patients (41.73%) had one or more postoperative complications, and there was no perioperative death. There was no statistically significant difference regarding postoperative complications between the diabetes group and the no diabetes group. Altogether, 120 patients (45.11%) experienced postoperative recurrence. Multivariate analysis revealed that diabetes was an independent risk factor for the recurrence (OR: 2.384, 95% CI: 1.065–5.336, p = 0.035), OS (HR: 1.597, 95% CI: 1.005–2.537, p = 0.047), and RFS (HR: 1.768, 95% CI: 1.068–2.925, p = 0.027) in AC patients after curative pancreatoduodenectomy. Conclusions: Diabetes may adversely affect the recurrence of patients with AC after curative pancreaticoduodenectomy, leading to an increased risk of poor prognosis in early-stage patients. Further studies involving a large sample size are needed to validate our results.
Virtual or In-Person: A Mixed Methods Survey to Determine Exercise Programming Preferences during COVID-19
Authors: Suderman Kirsten , Skene Tara , Sellar Christopher , Dolgoy Naomi , Pituskin Edith , Joy Anil A. , Culos-Reed Susan Nicole , McNeely Margaret L.
Published: September 2022

A survey was conducted to identify barriers and facilitators to engaging in virtual and in-person cancer-specific exercise during COVID-19. A theory-informed, multi-method, cross-sectional survey was electronically distributed to 192 individuals with cancer investigating preferences towards exercise programming during COVID-19. Respondents had previously participated in an exercise program and comprised two groups: those who had experience with virtual exercise programming (‘Virtual’) and those who had only taken part in in-person exercise (‘In-Person’). Quantitative data were summarized descriptively. Qualitative data were thematically categorized using framework analysis and findings were mapped to an implementation model. The survey completion response rate was 66% (N = 127). All respondents identified barriers to attending in-person exercise programming during COVID-19 with concerns over the increased risk of viral exposure. Virtual respondents (n = 39) reported: (1) feeling confident in engaging in virtual exercise; and (2) enhanced motivation, accessibility and effectiveness as facilitators to virtual exercise. In-Person respondents (n = 88) identified: (1) technology as a barrier to virtual exercise; and (2) low motivation, accessibility and exercise effectiveness as barriers towards virtual exercise. Sixty-six percent (n = 58) of In-Person respondents reported that technology support would increase their willingness to exercise virtually. With appropriately targeted support, perceived barriers to accessing virtual exercise—including motivation, accessibility and effectiveness—may become facilitators. The availability of technology support may increase the engagement of individuals with cancer towards virtual exercise programming.