bluuebunny/crossref_metadata_2025 huggingface dataset:
Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products
Authors: Lameei Abazar , Rahimi Ebrahim , Shakerian Amir , Momtaz Hassan
Published: April 2022

Background

Arcobacter spp. has been considered an emerging foodborne pathogen and a hazard to human health. The dairy chain has been isolated from different sources; nevertheless, data on Arcobacter occurrence in raw milk and dairy products in Iran are still scant.

Objective

The present study investigates the prevalence, antimicrobial susceptibility and the presence of virulence genes of Arcobacters species isolated from milk and dairy products.

Methods

Then, a total of 350 raw milk samples and 400 dairy product samples were collected from dairy supply centers in Isfahan, Iran. Presumptive Arcobacter strains were obtained by enriching samples in Oxoid Arcobacter enrichment broth (AEB) followed by the filtration of enrichment product through 0.45‐μm pore size membrane filters laid onto non‐selective blood at 30°C under microaerophilic conditions. Molecular identification of Arcobacter cryaerophilus and A. butzleri was performed by Polymerase chain reaction (PCR) amplification of the 16S rRNA gene, followed by sequencing. The disc diffusion method was used to determine the antimicrobial susceptibility of isolates. Targeted resistance and virulence genes were detected using multiplex PCR.

Results

The results show a low recovery rate of Arcobacter spp. in milk. Arcobacters were found in all types of milk, except raw camel milk, but were absent from all dairy products. Arcobacter butzleri was the predominant species in raw milk. Detection of virulence genes shows that all virulence genes targeted were found among A. butzleri, and six (cadF, cj1349, irgA, mviN, pldA, tlyA) were found among A. cryaerophilus. All A. butzleri strains and some A. cryaerophilus strains isolated from milk were resistant to amoxicillin‐clavulanic acid and tetracycline. All A. cryaerophilus isolates from milk were susceptible to gentamycin, streptomycin, erythromycin and ciprofloxacin. The distribution of resistance genes in Arcobacter strains in milk shows that all isolates carried tet(O) and blaOXA‐61 genes.

Conclusions

In conclusion, the results indicate a low recovery rate of Arcobacter spp. in milk and milk products. However, a significant number of Arcobacter strains with putative virulence genes may be potential pathogens for humans and an overall increase in Arcobacter resistance to first‐line antibiotics. These results highlight the need for regular surveillance of Arcobacter strains in milk and milk products in Iran.
Preparation and performance of low‐dielectric benzocyclobutene resins containing dicyclopentadiene
Authors: Wu Song , Peng Qiuxia , Deng Yueting , Shi Qingyu , Ma Jiajun , Huang Yawen , Yang Junxiao
Published: April 2022

Low‐dielectric‐constant polymer materials are widely used in the electronic industry owing to their unique characteristics. Benzocyclobutene (BCB) resin is one such material with excellent dielectric properties and thermal stabilities. In this work, a novel BCB‐functionalized monomer (DCPDNO‐BCB) was synthesized from 2,6‐dimethyl phenol‐DCPD novolac (DCPDNO) and 4‐Bromobenzocyclobutene through Ullmann coupling. The structure of DCPDNO‐BCB was characterized by 1H NMR, 13C NMR, and ESI‐MS, and poly(DCPDNO‐BCB), obtained by ring‐opening and subsequent Diels–Alder reaction at the appropriate temperature, was found to exhibit low dielectric properties (k = 2.53 at 10 MHz), low water absorption, high thermal stability (Td5 = 430°C), and good film flatness.
Humeral air sac neoplasm in a cockatiel (Nymphicus hollandicus)
Authors: Tyson Susan J. , Biswell Ethan
Published: April 2022

Primary respiratory neoplasms are uncommonly reported in avian species, and available literature on the occurrence of spontaneous neoplasms in birds is limited. Humeral air sac carcinomas are reported in large psittaciforms; however, no reports exist in cockatiels or other small psittaciforms. Psittaciforms have unique air sac anatomy compared to aquatic avian species and differences exist within the family Psittacidae. This report is the first to describe a humeral air sac neoplasm with pulmonary invasion in a cockatiel (Nymphicus hollandicus).
Target space entanglement in a matrix model for the bubbling geometry
Authors: Tsuchiya Asato , Yamashiro Kazushi
Published: April 2022

Abstract

We study the target space entanglement entropy in a complex matrix model that describes the chiral primary sector in $$ \mathcal{N} $$
N
= 4 super Yang-Mills theory, which is associated with the bubbling AdS geometry. The target space for the matrix model is a two-dimensional plane where the eigenvalues of the complex matrix distribute. The eigenvalues are viewed as the position coordinates of fermions, and the eigenvalue distribution corresponds to a droplet formed by the fermions. The droplet is identified with one that specifies a boundary condition in the bubbling geometry. We consider states in the matrix model that correspond to AdS5× S5, an AdS giant graviton and a giant graviton in the bubbling geometry. We calculate the target space entanglement entropy of a subregion for each of the states in the matrix model as well as the area of the boundary of the subregion in the bubbling geometry, and find a qualitative agreement between them.
On the Stokes-Type Resolvent Problem Associated with Time-Periodic Flow Around a Rotating Obstacle
Authors: Eiter Thomas
Published: April 2022

Consider the resolvent problem associated with the linearized viscous flow around a rotating body. Within a setting of classical Sobolev spaces, this problem is not well posed on the whole imaginary axis. Therefore, a framework of homogeneous Sobolev spaces is introduced where existence of a unique solution can be guaranteed for every purely imaginary resolvent parameter. For this purpose, the problem is reduced to an auxiliary problem, which is studied by means of Fourier analytic tools in a group setting. In the end, uniform resolvent estimates can be derived, which lead to the existence of solutions to the associated time-periodic linear problem.
Extending the viability of human precision-cut intestinal slice model for drug metabolism studies
Authors: Biel C. , Martinec O. , Sibering B. , van Summeren K. , Wessels A. M. A. , Touw D. J. , de Jong K. P. , de Meijer V. E. , Faber K. N. , Klooster J. P. ten , de Graaf I. A. M. , Olinga P.
Published: April 2022

Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS during traditional culture. We hypothesized that we could extend the hPCIS viability by using organoid medium. Therefore, we cultured hPCIS for up to 72 h in organoid media [expansion medium (Emed) and differentiation medium (Dmed)]. After incubation, we assessed culture-induced changes on viability markers, specific cell type markers and we assessed the metabolic activity of enterocytes by measuring midazolam metabolite formation. We show that the adenosine triphosphate (ATP)/protein ratio of Emed-cultured hPCIS and morphology of both Emed- and Dmed-cultured hPCIS was improved compared to WME-cultured hPCIS. Emed-cultured hPCIS showed an increased expression of proliferation and stem cell markers, whereas Dmed-cultured hPCIS showed an increased expression of proliferation and enterocyte markers, along with increased midazolam metabolism. Using the Emed, the viability of hPCIS could be extended for up to 72 h, and proliferating stem cells remained preserved. Using Dmed, hPCS also remained viable for up to 72 h, and specifically rescued the metabolizing enterocytes during culture. In conclusion, by using two different organoid culture media, we could extend the hPCIS viability for up to 72 h of incubation and specifically steer stem cells or enterocytes towards their original function, metabolism, and proliferation, potentially allowing pharmacokinetic and toxicology studies beyond the 24 h timeframe.
Rotations with Constant $$\mathbf {{\text {curl }}}$$ are Constant
Authors: Ginster Janusz , Acharya Amit
Published: April 2022

We address a problem that extends a fundamental classical result of continuum mechanics from the time of its inception, as well as answers a fundamental question in the recent, modern nonlinear elastic theory of dislocations. Interestingly, the implication of our result in the latter case is qualitatively different from its well-established analog in the linear elastic theory of dislocations. It is a classical result that if $$u\in C^2({\mathbb {R}}^n;{\mathbb {R}}^n)$$

u
∈

C
2

(

R

n

;

R

n

)

and $$\nabla u \in SO(n)$$

∇
u
∈
S
O
(
n
)

, it follows that u is rigid. In this article this result is generalized to matrix fields with non-vanishing $${\text {curl }}$$

curl

. It is shown that every matrix field $$R\in C^2(\varOmega ;SO(3))$$

R
∈

C
2

(
Ω
;
S
O

(
3
)

)

such that $${\text {curl }}R = constant$$

curl

R
=
c
o
n
s
t
a
n
t

is necessarily constant. Moreover, it is proved in arbitrary dimensions that a measurable rotation field is as regular as its distributional $${\text {curl }}$$

curl

allows. In particular, a measurable matrix field $$R: \varOmega \rightarrow SO(n)$$

R
:
Ω
→
S
O
(
n
)

, whose $${\text {curl }}$$

curl

in the sense of distributions is smooth, is also smooth.
Convergence Rate to the Tracy–Widom Laws for the Largest Eigenvalue of Wigner Matrices
Authors: Schnelli Kevin , Xu Yuanyuan
Published: April 2022

We show that the fluctuations of the largest eigenvalue of a real symmetric or complex Hermitian Wigner matrix of size N converge to the Tracy–Widom laws at a rate $$O(N^{-1/3+\omega })$$

O
(

N

-
1
/
3
+
ω

)

, as N tends to infinity. For Wigner matrices this improves the previous rate $$O(N^{-2/9+\omega })$$

O
(

N

-
2
/
9
+
ω

)

obtained by Bourgade (J Eur Math Soc, 2021) for generalized Wigner matrices. Our result follows from a Green function comparison theorem, originally introduced by Erdős et al. (Adv Math 229(3):1435–1515, 2012) to prove edge universality, on a finer spectral parameter scale with improved error estimates. The proof relies on the continuous Green function flow induced by a matrix-valued Ornstein–Uhlenbeck process. Precise estimates on leading contributions from the third and fourth order moments of the matrix entries are obtained using iterative cumulant expansions and recursive comparisons for correlation functions, along with uniform convergence estimates for correlation kernels of the Gaussian invariant ensembles.
The soluble form of CD160 acts as a tumor mediator of immune escape in melanoma
Authors: Gauci Marie-Léa , Giustiniani Jérôme , Lepelletier Clémence , Garbar Christian , Thonnart Nicolas , Dumaz Nicolas , Foussat Arnaud , Lebbé Céleste , Bensussan Armand , Marie-Cardine Anne
Published: April 2022

Melanoma is responsible for 90% of skin cancer-related deaths. Major therapeutic advances have led to a considerable improvement in the prognosis of patients, with the development of targeted therapies (BRAF or MEK inhibitors) and immunotherapy (anti-CTLA-4 or -PD-1 antibodies). However, the tumor constitutes an immunosuppressive microenvironment that prevents the therapeutic efficacy and/or promotes the development of secondary resistances. CD160 is an activating NK-cell receptor initially described as delineating the NK and CD8+T-cell cytotoxic populations. Three forms of CD160 have been described: (1) the GPI isoform, constitutively expressed and involved in the initiation of NK-cells' cytotoxic activity, (2) the transmembrane isoform, neo-synthesized upon cell activation, allowing the amplification of NK cells' cytotoxic functions and (3) the soluble form, generated after cleavage of the GPI isoform, which presents an immuno-suppressive activity. By performing immunohistochemistry analyses, we observed a strong expression of CD160 at the primary cutaneous tumor site of melanoma patients. We further demonstrated that melanoma cells express CD160-GPI isoform and constitutively release the soluble form (sCD160) into the tumor environment. sCD160 was shown to inhibit the cytotoxic activity of NK-cells towards their target cells. In addition, it was found in the serum of melanoma patients and associated with increased tumor dissemination. Altogether these results support a role for sCD160 in the mechanisms leading to the inhibition of anti-tumor response and immune surveillance in melanoma.
Comparison and Trends of Endovascular, Surgical and Hybrid Revascularizations and the Influence of Comorbidity in 1 Million Hospitalizations Due to Peripheral Artery Disease in Germany Between 2009 and 2018
Authors: Decker Josua A. , Helmer Magnus , Bette Stefanie , Schwarz Florian , Kroencke Thomas J. , Scheurig-Muenkler Christian
Published: April 2022

Objective

To analyze trends and differences of endovascular, surgical and hybrid revascularization approaches and the impact of comorbidity on characteristics, costs, and outcome of in-patients with peripheral artery disease (PAD) of the lower extremity.

Methods

Analyzing data provided by the Research Data Center of the German Federal Statistical Office, we included all hospitalizations due to PAD Fontaine IIb (Rutherford 2–3) or higher in Germany between 2009–2011 and 2016–2018. According to the individually performed procedures encoded by the Operation and Procedure Classification System, we divided hospitalizations by revascularization procedures into sole endovascular, sole surgical, hybrid, two-step and no revascularization. Patient’s comorbidity was assessed using the linear van Walraven comorbidity score (vWs).

Results

1,067,671 hospitalizations (mean age 71.3 ± 11.1 years; 60.1% male) were analyzed. Between 2009–2011 and 2016–2018, reimbursement costs rose by 28.0% from €2.72 billion (€5,350/case) to €3.49 billion (€6,238/case). The share of hospitalizations with any revascularization increased by 8.9% (67.7–73.7%) driven by an increase in two-step (+ 63.3%), hybrid (+ 58.2%) and sole endovascular revascularizations (+ 32.6%), while sole surgical approaches declined (− 18.2%). Hospitalizations of more comorbid patients (vWs ≥ 20) rose by 46.8% (21,444–31,478 cases), showed an overproportionate increase in costs of 124.6% (+ €1,750/case) and were associated with more individual procedures (+ 90.6%).

Conclusions

In-patient treatment of PAD patients shows increasing numbers of hybrid and sole endovascular revascularizations and more patients with higher comorbidity, while sole surgical interventions and in-hospital mortality decrease. Consequently, associated costs are surging especially in more comorbid patients due to an increasing number of performed procedures and escalation of therapy.
Assessment of cytochrome P450 3A4-mediated drug–drug interactions for ipatasertib using a fit-for-purpose physiologically based pharmacokinetic model
Authors: Jing Jing , Chen Yuan , Musib Luna , Jin Jin Y. , Cheung Kit Wun Kathy , Yoshida Kenta , Sane Rucha
Published: April 2022

Purpose

Ipatasertib, a potent and highly selective small-molecule inhibitor of AKT, is currently under investigation for treatment of cancer. Ipatasertib is a substrate and a time-dependent inhibitor of CYP3A4. It exhibits non-linear pharmacokinetics at subclinical doses in the clinical dose escalation study. To assess the DDI risk of ipatasertib at the intended clinical dose of 400 mg with CYP3A4 inhibitors, inducers, and substrates, a fit-for-purpose physiologically based pharmacokinetic (PBPK) model of ipatasertib was developed.

Methods

The PBPK model was constructed in Simcyp using in silico, in vitro, and clinical data and was optimized and verified using clinical data.

Results

The PBPK model described non-linear pharmacokinetics of ipatasertib and captured the magnitude of the observed clinical DDIs. Following repeated doses of 400 mg ipatasertib once daily (QD), the PBPK model predicted a 3.3-fold increase of ipatasertib exposure with itraconazole; a 2–2.5-fold increase with moderate CYP3A4 inhibitors, erythromycin and diltiazem; and no change with a weak CYP3A4 inhibitor, fluvoxamine. Additionally, in the presence of strong or moderate CYP3A4 inducers, rifampicin and efavirenz, ipatasertib exposures were predicted to decrease by 86% and 74%, respectively. As a perpetrator, the model predicted that ipatasertib (400 mg) caused a 1.7-fold increase in midazolam exposure.

Conclusion

This study demonstrates the value of using a fit-for-purpose PBPK model to assess the clinical DDIs for ipatasertib and to provide dosing strategies for the concurrent use of other CYP3A4 perpetrators or victims.
The impact of body mass index on prognosis in patients with colon carcinoma
Authors: Fuchs Julian , Schellerer Vera S , Brunner Maximilian , Geppert Carol I , Grützmann Robert , Weber Klaus , Merkel Susanne
Published: April 2022

Background

The impact of body mass index (BMI) on prognosis in patients with curatively resected stage I–III colon carcinoma was analyzed.

Methods

The prospectively collected data of 694 patients who underwent complete mesocolic excision between 2003 and 2014 were analyzed. BMI was classified into four categories: underweight (BMI < 18.5 kg/m2; n = 13), normal weight (BMI 18.5 to 24.9 kg/m2; n = 221), overweight (BMI 25.0 to 29.9 kg/m2; n = 309), and obese (BMI ≥ 30.0 kg/m2; n = 151). Univariate and multivariate analyses for comparison of prognosis were performed.

Results

The 5-year rate of locoregional recurrence in all 694 patients was 2.1%, and no differences were found with respect to BMI (p = 0.759). For distant metastasis, the 5-year rate for all patients was 13.4%, and BMI did not have a significant impact (p = 0.593). The 5-year rate of disease-free survival for all 694 patients was 72.4%. The differences with respect to BMI were not found to be significant in univariate analysis (p = 0.222). In multivariate Cox regression analysis, disease-free survival was significantly better in obese patients (HR 0.7; p = 0.034). Regarding overall survival, the 5-year rate for all patients was 78.1%. In univariate analyses, no significant differences were found for BMI (p = 0.094). In the Cox regression analysis, overweight and obese patients had significantly better survival (overweight: HR 0.7; p = 0.027; obese: HR 0.6; p = 0.019).

Conclusion

The better survival of overweight and obese patients in multivariate analyses must be interpreted with caution. It is influenced by several factors and seems to correspond to the phenomenon of the obesity paradox.
On a virtual element formulation for trusses and beams
Authors: Wriggers P. 
Published: April 2022

The virtual element method (VEM) was developed not too long ago, starting with the paper [2] related to elasticity in solid mechanics. The virtual element method allows to revisit the construction of different elements; however, it has so far not applied to one-dimensional structures like trusses and beams. Here we study several VEM elements suitable for trusses and beams and show that the virtual element methodology produces elements that are equivalent to well-known finite elements but also elements that are different, especially for higher-order ansatz functions. It will be shown that these elements can be easily incorporated in classical finite element codes since they have the same number of unknowns as finite beam elements. Furthermore, the formulation allows to compute nonlinear structural problems undergoing large deflections and rotations.
Nuclear survivin is a prognosticator in gastroenteropancreatic neuroendocrine neoplasms: a meta-analysis
Authors: Krieg Sarah , Roderburg Christoph , Fung Stephen , Luedde Tom , Knoefel Wolfram Trudo , Krieg Andreas
Published: April 2022

Purpose

Gastroenteropancreatic neuroendocrine neosplasms (GEP-NEN) are biologically heterogenous tumors with an increasing incidence over the past decades. Although efforts have been made in the treatment of these tumors, survival rates in metastasized tumor stages remain frustrating. Thus, there is an urgent need to identify novel targets as alternative treatment options. In this regard, the inhibitor of apoptosis protein (IAP) family member survivin could be such an attractive target. Therefore, aim of our meta-analysis was to assess the role of survivin as a biomarker and predictor in GEP-NEN.

Methods

Medline, Web of Science and Scopus were screened for studies that fulfilled our selection criteria. Quality assessement of the studies was based on design, methodology, generalizability and results analysis. Meta-analyses were conducted using a random-effects model and effect size measures were expressed as pooled Hazard Ratio (HR) or Odds Ratio (OR) with 95% Confidence Interval (CI).

Results

Six eligible studies with 649 patients (range 77–132) assessed survivin expression in GEP-NEN by immunohistochemistry. High expression levels of nuclear survivin in GEP-NEN correlated with a shorter overall survival (HR 3.10; 95% CI 2.15–4.47; p < 0.0001). In contrast to cytoplasmic survivin (OR 1.24; CI 0.59–2.57; p = 0.57), nuclear survivin was also associated (OR 15.23; CI 3.61–64.23; p = 0.0002) with G3/poorly differentiated GEP-NEN.

Conclusion

Nuclear Survivin is highly expressed in more aggressive G3 GEP-NEN and correlates with a poor outcome. Survivin is therefore an interesting molecule for a targeted therapy, especially for patients with highly proliferative G3 GEP-NENs.
Addressing symptoms that affect patients’ eating according to the Head and Neck Patient Symptom Checklist©
Authors: Granström Brith , Holmlund Thorbjörn , Laurell Göran , Fransson Per , Tiblom Ehrsson Ylva
Published: April 2022

Purpose

The purpose of this prospective study was to assess which nutritional impact symptoms (NIS) interfere with oral intake in patients with head and neck cancer (HNC) and how the symptoms interfere with body weight loss, up to 1 year after treatment.

Methods

This was a prospective study of 197 patients with HNC planned for treatment with curative intention. Body weight was measured before the start of treatment, at 7 weeks after the start of treatment, and at 6 and 12 months after completion of treatment. NIS and NIS interfering with oral intake at each follow-up were examined with the Head and Neck Patient Symptom Checklist©(HNSC©).

Results

At 7 weeks of follow-up, patients experienced the greatest symptom and interference burden, and 12 months after treatment the NIS scorings had not returned to baseline. One year after treatment, the highest scored NIS to interfere with oral intake was swallowing problems, chewing difficulties, and loss of appetite. At all 3 follow-ups, the total cumulative NIS and NIS interfering with oral intake were associated with body weight loss. Factors increasing the risk for a body weight loss of ≥ 10% at 12 months after treatment were pain, loss of appetite, feeling full, sore mouth, difficulty swallowing, taste changes, and dry mouth. Women scored higher than men in NIS and NIS interfering with oral intake. Furthermore, during the study period about half of the population had a body weight loss > 5%.

Conclusion

Because both nutritional and clinical factors may affect body weight, this study highlights the importance of a holistic approach when addressing the patients’ nutritional issues.

Trial registration

ClinicalTrials.gov NCT03343236, date of registration: November 17, 2017.
Scoping review on trauma and recovery in youth after natural disasters: what Europe can learn from natural disasters around the world
Authors: Witt Andreas , Sachser Cedric , Fegert Jörg M.
Published: April 2022

In the last decade, Europe has seen a rise in natural disasters. Due to climate change, an increase of such events is predicted for the future. While natural disasters have been a rare phenomenon in Europe so far, other regions of the world, such as Central and North America or Southeast Asia, have regularly been affected by Hurricanes and Tsunamis. The aim of the current study is to synthesize the literature on child development in immediate stress, prolonged reactions, trauma, and recovery after natural disasters with a special focus on trajectories of (mal-)adaptation. In a literature search using PubMed, Psychinfo and EBSCOhost, 15 studies reporting about 11 independent samples, including 11,519 participants aged 3–18 years, were identified. All studies identified resilience, recovery, and chronic trajectories. There was also evidence for delayed or relapsing trajectories. The proportions of participants within each trajectory varied across studies, but the more favorable trajectories such as resilient or recovering trajectory were the most prevalent. The results suggested a more dynamic development within the first 12 months post-disaster. Female gender, a higher trauma exposure, more life events, less social support, and negative coping emerged as risk factors. Based on the results, a stepped care approach seems useful for the treatment of victims of natural disasters. This may support victims in their recovery and strengthen their resilience. As mental health responses to disasters vary, a coordinated screening process is necessary, to plan interventions and to detect delayed or chronic trauma responses and initiate effective interventions.
Schlaganfallpfad Tirol
Authors: Boehme Christian , Krebs Stefan , Geley Theresa , Rinner Heinrich , Maurer Andreas , Runge Julia , Schoech Johannes , Willeit Johann , Kiechl Stefan , Knoflach Michael
Published: April 2022

Hintergrund

In der Behandlung des Schlaganfalls gibt es Fortschritte auf vielen Ebenen. Dies führt zu besseren Heilungschancen und einer Minderung körperlicher Beeinträchtigung. Akuttherapiemaßnahmen sind auf ein kurzes Zeitfenster nach Auftreten limitiert, deshalb ist das Notfallmanagement besonders kritisch. Das Projekt Schlaganfallpfad Tirol wurde realisiert, um die Versorgungskette beim Krankheitsbild Schlaganfall von der prähospitalen Phase bis zum Abschluss der Rehabilitation zu optimieren.

Ziel der Arbeit

Beschreibung des Tiroler Schlaganfallpfads als Beispiel für die Optimierung der Schlaganfallversorgung in einer mitteleuropäischen alpinen Region mit Schwerpunkt auf die prähospitale Versorgung.

Material und Methoden

In vier Teilprojekten von der Prähospitalphase bis zur Nachbehandlung wurden Versorgungsprozesse und Schnittstellen optimiert und mit Qualitätssicherungsmaßnahmen evaluiert.

Ergebnisse

Nach Implementierung hat sich die Thrombolyserate fast verdoppelt und die Rate an gutem funktionellem Outcome nach 3 Monaten verbessert. Komplikationen wie eine Aspirationspneumonie haben deutlich abgenommen. Der Zugang zu rehabilitativen Maßnahmen hat sich verbessert, insbesondere auch wegen des Aufbaus einer qualitätskontrollierten und finanzierten ambulanten Rehabilitation.

Schlussfolgerung

Ein ganzheitliches Versorgungsprojekt kann gut in die Praxis umgesetzt werden und verbessert die Versorgungsqualität beim ischämischen Schlaganfall. Der europäische Aktionsplan Schlaganfall 2018–2030 empfiehlt die Etablierung umfassender Schlaganfallpfade in allen Regionen und Ländern Europas.
Patterns, drivers and detectability of infestation symptoms following attacks by the European spruce bark beetle
Authors: Kautz Markus , Peter Felix J. , Harms Laura , Kammen Stephan , Delb Horst
Published: April 2022

Recent outbreaks of the European spruce bark beetle (Ips typographus) in Norway spruce (Picea abies) forests in Central Europe highlight the importance of timely detection and sanitation of infested trees for pest management efficacy. This study provides novel quantitative evidence on the manifestation of infestation symptoms and their visual detectability, to guide accelerated, optimized terrestrial bark beetle monitoring, as well as establishing benchmarks for potential alternative (e.g. sensor-based) monitoring approaches. We employed bi-weekly, individual tree-level assessments on 85 hectares of spruce-dominated unmanaged forest over a 2-year period in south-western Germany (detecting a total of 1,176 infested trees). By applying decision tree-type models, we quantified the predictive power of observed symptoms and their correlation with environmental factors and time. Terrestrial detection accuracy and timeliness were high, suggestive of being sufficient to suppress I. typographus outbreak propagation by subsequent sanitation felling. Among the six studied symptoms, boring dust occurred most frequently (in 82% of correctly detected infestations) and is most suitable for timely detection. Total symptom abundance was best explained by two site parameters (slope, Standardized Precipitation-Evapotranspiration-Index) and I. typographus population density, while it was widely independent of tree parameters and time. Though individual symptoms varied over time and among trees, patterns were clearly identified. For instance, infestations in spring were most critical to be timely detected, while increasing crown discoloration and defoliation facilitated detection in late summer and autumn. Findings further imply that hibernation trees would optimally be detected already in late summer with sanitation felling applied before November.
Preparation and benchmarking of novel cellulose nanopaper
Authors: Kargupta Wriju , Seifert Reanna , Martinez Mark , Olson James , Tanner Joanne , Batchelor Warren
Published: April 2022

Synthetic polymers and plastics which are currently used as barrier materials in packaging applications are neither renewable nor biodegradable. Nanopaper, which is obtained by breaking down cellulose fibers into nanoscale particles, have unique properties with the potential to replace synthetic packaging materials, but requires very high energy to mechanically process the fibers into nanopaper. This research investigates whether refining alone can be used to produce nanopaper with sufficient quality for packaging applications. Nanopaper was produced from Bleached Eucalyptus Kraft (BEK) refined with a PFI mill and from Northern Bleached Softwood Kraft (NBSK) refined in a pilot disc refiner. Both trials found a plateau for oxygen permeability and water vapour permeability that was reached after 1800 kWh/t and 12,000 kWh/t for refining in the pilot disc refiner and PFI mill, respectively. Refining beyond these optima produced either little or no reduction in permeability, while increasing the drainage time to form a sheet. However, elastic modulus, strain at break and sheet light transmittance did continue to increase. The plateau oxygen permeability of ~ 1.24 (cc µm)/(m2 day kPa) is 1–3 orders of magnitude lower than the oxygen permeability for PET and LDPE, respectively, while the plateau water vapour permeability ~ 3 × 10–11 g/m.s. Pa was 1–2 orders of magnitude higher than for PET and LDPE. The improved strength and barrier properties of nanopaper achieved at lab and pilot scale mechanical refining process promises a sustainable alternative to conventional packaging.

Graphical abstract
The Influence of Indirect Democracy and Leadership Choice on Cooperation
Authors: Schories Fanny E.
Published: April 2022

The paper examines whether an institution has a differing impact on cooperation if it is introduced by a representative of the affected subjects rather than exogenously imposed. The experimental design controls for selection effects arising from the endogenous policy choice. The treatment varies whether the decision-maker is elected or randomly appointed. There is evidence of a large democracy premium in the sense that endogenously chosen institutions lead to more cooperation than identical exogenous institutions, but only if the group leader is democratically chosen. Especially the subjects who initially did not prefer the policy are more likely to cooperate if it was brought about by an elected representative. There is no democracy premium for randomly appointed group leaders.
Economic analysis of international environmental agreements: lessons learnt 2000–2020
Authors: Pouw Nicky R. M. , Weikard Hans-Peter , Howarth Richard B.
Published: April 2022

On the occasion of the 20th anniversary of International Environmental Agreements: Politics, Law & Economics, we conduct an extensive review of papers published in this journal that address the economic dimensions of international environmental agreements (IEAs). We focus particularly on the lessons learnt from this body of literature and the implications for the assessment and design of IEAs in relation to goals such as efficiency, effectiveness, and equity. Our key conclusions run as follows. First, at the international level, universal coalitions are more cost-efficient and effective than fragmented regimes, but more difficult to negotiate and less stable. Second, in developing countries, there is need for substantial external funding to cover the short-run costs of environmental compliance. Third, market-based solutions have been increasingly applied in international agreements but with mixed results. For example, cap-and-trade systems have the potential to achieve greenhouse gas emissions reductions and least economic cost. But in the provisioning of water services, private sector solutions often result in outcomes that are unaffordable for low-income groups or nonviable for businesses, suggesting well-designed public–private partnerships. At the international level, Green Bond markets can attract investors for climate and environmental projects, but implementation failures tend to weaken outcomes. Finally, in practical politics, economically optimal designs are rarely achieved. Future applied economic research should therefore critically investigate institutions and the scope for their reform. Gains in knowledge are expected to come from economic analyses taking a broader perspective on “the economy”, taking institutions and social and ecological relations into account from the start.
Sentimental and spatial analysis of COVID-19 vaccines tweets
Authors: Umair Areeba , Masciari Elio
Published: April 2022

The world has to face health concerns due to huge spread of COVID. For this reason, the development of vaccine is the need of hour. The higher vaccine distribution, the higher the immunity against coronavirus. Therefore, there is a need to analyse the people’s sentiment for the vaccine campaign. Today, social media is the rich source of data where people share their opinions and experiences by their posts, comments or tweets. In this study, we have used the twitter data of vaccines of COVID and analysed them using methods of artificial intelligence and geo-spatial methods. We found the polarity of the tweets using the TextBlob() function and categorized them. Then, we designed the word clouds and classified the sentiments using the BERT model. We then performed the geo-coding and visualized the feature points over the world map. We found the correlation between the feature points geographically and then applied hotspot analysis and kernel density estimation to highlight the regions of positive, negative or neutral sentiments. We used precision, recall and F score to evaluate our model and compare our results with the state-of-the-art methods. The results showed that our model achieved 55% & 54% precision, 69% & 85% recall and 58% & 64% F score for positive class and negative class respectively. Thus, these sentimental and spatial analysis helps in world-wide pandemics by identify the people’s attitudes towards the vaccines.
Syntheses and Structures of [Fe(TPA)X2](ClO4) and [{Fe(TPA)Y}2O](ClO4)2 Where TPA = Tris-(2-pyridylmethyl)amine, X = N3, or Br, and Y = N3, Br, NCO, or NCS
Authors: Xue Jia , Hangun-Balkir Yelda , Mullaney Matthew , Nadir Shamila , Lewis Justin Cody , Henry-Smith Christine , Jayaratna Naleen B. , Kawshalya Kumarihami C. A. U. , Norman Richard E.
Published: April 2022

[Fe(TPA)(N3)2](ClO4) and [Fe(TPA)Br2](ClO4), where TPA is tris-(2-pyridylmethyl)amine, crystallize in the monoclinic space group P21/c with a = 8.7029(5) Å, b = 19.168(1) Å, c = 13.5728(7) Å, β = 101.472(3)°, and a = 8.944(3) Å, b = 16.578(6) Å, c = 15.108(6) Å, β = 103.18(2)°, respectively. The structures were determined at 150 K from 3397 reflections (1426 observed) with R = 0.063 (Rw = 0.097), and at 115 K from 5617 reflections (2261 observed) with R = 0.057 (Rw = 0.065), respectively. In both cases, the iron is pseudo-octahedral with the two halide/pseudohalide ions cis. The Fe–X bond trans to the tertiary amine is shorter. The structures of [{Fe(TPA)X}2O](ClO4)2 where X = N3, Br, NCO, and two polymorphic forms of NCS, are also reported. The azide derivative [CH3CN solvate, monoclinic P21/n, a = 11.8038(11) Å, b = 22.547(2) Å, c = 17.344(2) Å, β = 106.972(4)°, determined at 100 K from 8972 reflections (4404 observed) with R = 0.087 (Rw = 0.145)] has two distinct Fe environments—the tertiary amine is cis to the oxido bridge at one site and is trans to the oxido bridge at the other site; the trans Fe–N3° distance is longer. Both the Br and NCO derivatives are monoclinic, C2/c [with a = 16.1480(17), b = 17.2036(13), c = 16.8521(12), β = 111.204(10), data collected at 293 K, 3753 reflections (2404 observed), R = 0.069 (Rw = 0.151), and a = 15.7470(9), b = 18.2270(11), c = 16.8950(8), β = 110.666(3), data collected at 90 K, 5392 reflections (3028 observed), R = 0.064 (Rw = 0.091), respectively]. Both polymorphs of the NCS derivative are monoclinic—one is P21/c and the other P21/n [a = 11.075(2), b = 15.436(2), c = 12.351(2), β = 95.528(7), data collected at 90 K, 5378 reflections (4345 observed), R = 0.068 (Rw = 0.198), and a = 12.396(2), b = 15.428(3), c = 44.505(8), β = 95.211(7), data collected at 110 K, 16,527 reflections (6540 observed), R = 0.069 (Rw = 0.105), respectively]. For the Br, NCO and NCS dimers, each iron of the [{Fe(TPA)X}2O]2+ unit is pseudo-octahedral with the halide/pseudohalide and oxide ions cis. The oxide bridge is linear, and the two halides/pseudohalides are anti. The ranking of trans influence of the ligands is O2− ≫ Br− > Cl− > N3− > NCO− ≥ NCS− > pyridyl > tertiary amine and the ranking of cis influence of the ligands is O2− ≫ N3− > NCO− > Cl− ≥ Br− > NCS−.

Graphical Abstract

The X-ray structures of two monomeric [Fe(TPA)(X)2](ClO4), where TPA is tris-(2-pyridylmethyl)amine and X = N3, and Br, and four dimeric [{Fe(TPA)Y}2O](ClO4)2, where Y =N3, Br, NCO, and NCS are presented and discussed.
A Tri-Directional Examination of Parental Personality, Parenting Behaviors, and Contextual Factors in Influencing Adolescent Behavioral Outcomes
Authors: Truhan Tayler E. , Sedikides Constantine , McIlvenna Micheala , Andrae Lena , Turner Rhiannon N. , Papageorgiou Kostas A.
Published: April 2022

Links between parental personality, parenting, and adolescent behavior have been well established. However, extant research is limited by the sole focus on parental Big Five personality, and not taking home and family context into account. These gaps were addressed in two studies. In study 1, context, parental personality, and their interactions were examined as predictors of parenting in separate mother and father models (parents only). In study 2, context, parental personality, and parenting were examined as predictors of adolescent behavioral outcomes (parent–adolescent dyads). Parents (N = 283, 45.6% mothers, Mage = 45.51 years) completed assessments of socioeconomic status (SES), adverse childhood experiences (ACEs), personality (Big Five, Dark Triad), and parenting. Adolescents (N = 257, 51.4% female, Mage = 13.65 years) completed an assessment of behavior. Parent Dark Triad domains explained more variance in parental warmth and hostility than the Big Five, but equivalent variance in adolescent behavior. SES interacted with maternal personality, whereas ACEs interacted with paternal personality, to predict parenting behavior. The results showcase the importance of assessing a wider spectrum of parental personality, and examining contextual factors, in affecting adolescent development.
On testimonial knowledge and its functions
Authors: Croce Michel
Published: April 2022

The problem of explaining how we acquire knowledge via testimony gives rise to a dilemma, according to which any theory must make testimonial knowledge either too hard or too easy, and therefore no adequate account of testimonial knowledge is possible. In recent work (2020), John Greco offers a solution to the dilemma on behalf of anti-reductionism that appeals to Edward Craig’s functionalist epistemology. It is argued that Greco’s solution is flawed, in that his functionalist account provides wrong verdicts of ordinary cases of testimonial knowledge. In contrast, it is shown that both anti-reductionism and reductionism have the resources to solve the dilemma and provide the right verdicts in ordinary cases of testimonial knowledge without appealing to Greco’s functionalism.
Science advice: making credences accurate
Authors: Blessenohl Simon , Sarikaya Deniz
Published: April 2022

Policy-makers often rely on scientists to inform their decisions. When advising policy-makers, what should scientists say? One view says that scientists ought to say what they have a high credence in. Another view says that scientists ought to say what they expect to lead to good policy outcomes. We explore a third view: scientists ought to say what they expect to make the policy-makers’ credences accurate.
Group belief reconceived
Authors: de Ridder Jeroen
Published: April 2022

An influential account or group belief analyzes it as a form ofjoint commitmentby group members. In spite of its popularity, the account faces daunting objections. I consider and reply to two of them. The first, due to Jennifer Lackey, is that the joint commitment account fails as an account of groupbeliefsince it cannot distinguish groupbeliefsfrom groupliesandbullshit. The second is that the joint commitment account fails because it makes group beliefvoluntary, whereas genuine belief isinvoluntary. I propose an amendment to the basic joint commitment account which offers a unified reply to both objections. Although my novel account of group belief departs from the basic joint commitment account, it retains its spirit. The account entails that genuine group belief is much rarer than proponents of the joint commitment account have hitherto realized.
Language games and the emergence of discourse
Authors: Barrett Jeffrey A. , VanDrunen Jacob
Published: April 2022

Wittgenstein (Philosophical investigations, Basil Blackwell Ltd., 1958) used the notion of a language game to illustrate how language is interwoven with action. Here we consider how successful linguistic discourse of the sort he described might emerge in the context of a self-assembling evolutionary game. More specifically, we consider how discourse and coordinated action might self-assemble in the context of two generalized signaling games. The first game shows how prospective language users might learn to initiate meaningful discourse. The second shows how more subtle varieties of discourse might co-emerge with a meaningful language.
Relationship between temporal rhythm-based classification of atrial fibrillation and stroke: real-world vs. clinical trial
Authors: Ding Wern Yew , Rivera-Caravaca José Miguel , Marin Francisco , Roldán Vanessa , Lip Gregory Y. H.
Published: April 2022

Background

The risk of stroke according to clinical classification of atrial fibrillation (AF) remains poorly defined. Here, we assessed the impact of AF type on stroke risk in vitamin K antagonist-treated patients with AF in ‘real-world’ and ‘clinical trial’ cohorts.

Methods

Post-hoc analysis of patient-level data from the Murcia AF Project and AMADEUS trial. Clinical classification of AF was based on contemporary recommendations from international guidelines. Study endpoint was the incidence rate of ischaemic stroke. Stroke risk was determined using CHA2DS2-VASc score and CARS. A modified CHA2DS2-VAS‘c’ score that applied one additional point for a ‘c’ criterion of continuous AF (i.e. non-paroxysmal AF) was calculated.

Results

We included 5,917 patients: 1,361 (23.0%) real-world and 4,556 (77.0%) clinical trial. Baseline demographics were balanced in the real-world cohort but clinical trial participants with non-pAF (vs. pAF) were older, male-predominant and had more comorbidities. Crude stroke rates were comparable between the groups in real-world patients (IRR 0.72 [95% CI,0.37-1.28], p = 0.259) though clinical trial participants with non-pAF had a significantly higher crude rate of stroke events (IRR 4.66 [95%,CI,2.41-9.48], p < 0.001). Using multivariable analysis, AF type was not independently associated with stroke risk in the real-world (adjusted HR 1.41 [95% CI,0.80-2.50], p = 0.239) and clinical trial (adjusted HR 1.16 [95% CI,0.62-2.20], p = 0.646) cohorts, after accounting for other risk factors. There was no significant improvement in the CHA2DS2-VAS‘c’ compared to CHA2DS2-VASc score in either cohorts (p > 0.05).

Conclusions

Overall, our results support the need for anticoagulation based on thromboembolic risk profile rather than AF type.
EMG analysis across different tasks improves prevention screenings in diabetes: a cluster analysis approach
Authors: Piatkowska Weronika , Spolaor Fabiola , Guiotto Annamaria , Guarneri Gabriella , Avogaro Angelo , Sawacha Zimi
Published: April 2022

The aim of this work was twofold: on one side to determine the most suitable parameters of surface electromyography (sEMG) to classify diabetic subjects with and without neuropathy and discriminate them from healthy controls and second to assess the role of the task acquired in the classification process. For this purpose 30 subjects were examined (10 controls, 10 diabetics with and 10 without neuropathy) whilst walking and stair ascending and descending. The electrical activity of six muscles was recorded bilaterally through a 16-channel sEMG system synchronised with a stereophotogrammetric system: Rectus Femoris, Gluteus Medius, Tibialis Anterior, Peroneus Longus, Gastrocnemius Lateralis and Extensor Digitorum. Spatiotemporal parameters of gait and stair climbing and the following sEMG parameters were extracted: signal envelope, activity duration, timing of activation and deactivation. A hierarchical clustering algorithm was applied to the whole set of parameters with different distances and linkage methods. Results showed that only by applying the Ward agglomerative hierarchical clustering (Hamming distance) to the all set of parameters extracted from both tasks, 5 well-separated clusters were obtained: cluster 3 included only DS subjects, cluster 2 and 4 only controls and cluster 1 and 5 only DNS subjects. This method could be used for planning rehabilitation treatments.

Graphical abstract
Historically transposed flipper pairs in a mounted plesiosaurian skeleton
Authors: Krahl Anna , Smith Adam S. , Werneburg Ingmar
Published: April 2022

Plesiosaurians evolved four wing-like flippers that are morphologically similar to each other and were most likely used in underwater flight. Plesiosaurians have been the subject of a long research history as well as a long history of misidentifications and misinterpretations, especially transposition of parts of or entire fore- and hind flippers. We identified the transposed fore- and hind flippers in a mountedCryptoclidus eurymerusspecimen (GPIT-PV-30092) on display in the Paleontological Collection of Tübingen University. It is likely that the fore- and hind flippers were accidentally transposed when the skeleton was mounted, although, amongst plesiosaurians, the fore- and hind flippers ofCryptoclidus eurymerusare some of the least similar-looking ones. This occurred either during a remounting of the skeleton from a free-standing armature on the ground to a freely “flying” skeleton hanging from the ceiling, or after a research project conducted on the specimen in the 1970s. We summarize osteological characteristics that can be used to correctly identify fore- and hind flippers of this species, and for better future assessment of the plesiosaurian locomotory system.