bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterisation of the Complete Mitochondrial Genome of Critically Endangered Mustela lutreola (Carnivora: Mustelidae) and Its Phylogenetic and Conservation Implications
Authors: Skorupski Jakub
Published: January 2022

In this paper, a complete mitochondrial genome of the critically endangered European mink Mustela lutreola L., 1761 is reported. The mitogenome was 16,504 bp in length and encoded the typical 13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes, and harboured a putative control region. The A+T content of the entire genome was 60.06% (A > T > C > G), and the AT-skew and GC-skew were 0.093 and −0.308, respectively. The encoding-strand identity of genes and their order were consistent with a collinear gene order characteristic for vertebrate mitogenomes. The start codons of all protein-coding genes were the typical ATN. In eight cases, they were ended by complete stop codons, while five had incomplete termination codons (TA or T). All tRNAs had a typical cloverleaf secondary structure, except tRNASer(AGC) and tRNALys, which lacked the DHU stem and had reduced DHU loop, respectively. Both rRNAs were capable of folding into complex secondary structures, containing unmatched base pairs. Eighty-one single nucleotide variants (substitutions and indels) were identified. Comparative interspecies analyses confirmed the close phylogenetic relationship of the European mink to the so-called ferret group, clustering the European polecat, the steppe polecat and the black-footed ferret. The obtained results are expected to provide useful molecular data, informing and supporting effective conservation measures to save M. lutreola.
Acceptability of Community-Based Tuberculosis Preventive Treatment for People Living with HIV in Zimbabwe
Authors: Msukwa Martin K. , Mapingure Munyaradzi P. , Zech Jennifer M. , Masvawure Tsitsi B. , Mantell Joanne E. , Musuka Godfrey , Apollo Tsitsi , Boccanera Rodrigo , Chingombe Innocent , Gwanzura Clorata , Howard Andrea A. , Rabkin Miriam
Published: January 2022

As Zimbabwe expands tuberculosis preventive treatment (TPT) for people living with HIV (PLHIV), the Ministry of Health and Child Care is considering making TPT more accessible to PLHIV via less-intensive differentiated service delivery models such as Community ART Refill Groups (CARGs). We designed a study to assess the feasibility and acceptability of integrating TPT into CARGs among key stakeholders, including CARG members, in Zimbabwe. We conducted 45 key informant interviews (KII) with policy makers, implementers, and CARG leaders; 16 focus group discussions (FGD) with 136 PLHIV in CARGs; and structured observations of 8 CARG meetings. KII and FGD were conducted in English and Shona. CARG observations were conducted using a structured checklist and time-motion data capture. Ninety six percent of participants supported TPT integration into CARGs and preferred multi-month TPT dispensing aligned with ART dispensing schedules. Participants noted that the existing CARG support systems could be used for TB symptom screening and TPT adherence monitoring/support. Other perceived advantages included convenience for PLHIV and decreased health facility provider workloads. Participants expressed concerns about possible medication stockouts and limited knowledge about TPT among CARG leaders but were confident that CARGs could effectively provide community-based TPT education, adherence monitoring/support, and TB symptom screening provided that CARG leaders received appropriate training and supervision. These results are consistent with findings from pilot projects in other African countries that are scaling up both differentiated service delivery for HIV and TPT and suggest that designing contextually appropriate approaches to integrating TPT into less-intensive HIV treatment models is an effective way to reach people who are established on ART but who may have missed out on access to TPT.
Ultrasonographic Changes of Abdominal Muscles in Subjects with and without Chronic Low Back Pain
Authors: Da Cuña-Carrera Iria , Alonso-Calvete Alejandra , Lantarón-Caeiro Eva M. , Soto-González Mercedes
Published: January 2022

Chronic low back pain (CLBP) is a prevalent disfunction in the spine, affecting both women and men. The implication of the abdominal muscles in this disfunction has been studied, including wrong breathing patterns or inactivity of this area. However, there is a lack of studies examining changes in thickness of abdominal with ultrasonography. Thus, the aim of this study is to analyze the differences in the thickness of abdominal muscles at rest and during breathing between subjects with and without CLBP. A total of 72 subjects were divided in two groups: participants with CLBP (n = 36) and participants without CLBP (n = 36). In both groups, the thickness of the four abdominal muscles was measured and compared at rest and during breathing with ultrasonography. In TrA and IO there were no significant differences between groups, but those subjects with CLBP increased the muscle thickness more than participants without pain during breathing. In EO there were no differences in muscle thickness between groups and between rest and breathing. In RA, subjects with CLBP showed less muscle thickness than subjects without pain during breathing, but no changes were found at rest. In conclusion, the deepest abdominal muscles, TrA and IO, appear to increase their thickness and RA appear to decrease more in subjects with CLBP, in comparison with healthy participants.
The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway
Authors: Fotirić Akšić Milica , Cerović Radosav , Hjeltnes Stein Harald , Meland Mekjell
Published: January 2022

This study evaluated the effective pollination period (EPP) in four European plum (Prunus domestica L.) cultivars (‘Mallard’, ‘Edda’, ‘Jubileum’, and ‘Reeves’) during two years (2018–2019) under the environmental conditions in western Norway. The pollination of plum cultivars was carried out one, three, five, seven, and nine days after anthesis (DAA) with a pollen mix of two compatible cultivars (‘Victoria’ and ‘Opal’). Initial, middle-season, and final fruit set was recorded after one month and two months after pollination and just before the harvest, respectively. On average from both years cultivar ‘Jubileum’ had the highest fruit set when pollinated one, three, five, seven, and nine DAA (33.23%, 30.83%, 8.47%, 3.08%, and 1.15%, respectively), which was more than two folds higher fruit set than in the other studied cultivars. Cultivar ‘Jubileum’ showed significantly reduced fruit set between pollination on five and nine DAA, while cultivars ‘Mallard’, ‘Edda’, and ‘Reeves’ had markedly reduced fruit set if pollinated three to five DAA, implying that the EPP in ‘Jubileum’ was five days while in the rest it was three days. Variation of weather conditions during the flowering period in both years did not have a major effect on the receptivity of stigmas in the studied plum cultivars, which means that the existing differences in the length of EPP is maternal-genotype dependent.
Light-Intercepting Characteristics and Growth of Tomatoes Cultivated in a Greenhouse Using a Movable Bench System
Authors: Ohashi Yuta , Murai Misato , Ishigami Yasuhiro , Goto Eiji
Published: January 2022

The objective of this study was to investigate the growth and light-intercepting characteristics of tomatoes when movable benches are used in their cultivation. We cultivated tomatoes in a greenhouse (168 m2) during summer (9 July–9 September 2018) under different furrow distances (F1.0 = 1.0 m and F1.6 = 1.6 m) and movable benches (M indicates that the furrow distance = 0.4–0.8 m). Compared to the other treatments, when the movable bench was used to the change furrow distance depending on the plant growth stage (M treatment), the percentage of canopy light interception increased to ~90% at the early stage of plant growth (~20 days after transplanting). The percentage of canopy light interception for different treatments increased in the order of M > F1.0 > F1.6, and it increased towards the end of cultivation. In addition, the yield per unit area exhibited the same trend. Therefore, the solar radiation inside a greenhouse can be efficiently intercepted by plants when movable benches are used. This indicated that it was possible to increase plant yield per unit area using movable benches in plant cultivation.
Correlation of Soil Characteristics and Citrus Leaf Nutrients Contents in Current Scenario of Layyah District
Authors: Ahmad Niaz , Hussain Sajjad , Ali Muhammad Arif , Minhas Asif , Waheed Waqar , Danish Subhan , Fahad Shah , Ghafoor Umber , Baig Khurram Shehzad , Sultan Haider , Hussain Muhammad Iftikhar , Ansari Mohammad Javed , Marfo Theodore Danso , Datta Rahul
Published: January 2022

Soil with low fertility is a big problem for achieving citrus productivity. In this regard, the management of macro and micronutrients is essential. Macro and micronutrient deficiency decreased the yield and the quality of citrus fruit. It is the need of the hour to classify the soil fertility status under changing climatic scenarios. The current soil fertility survey was conducted to examine the macro and micronutrient status in the citrus production area. In soil, three depths (0–15, 15–30, and 30–45 cm) were taken for sampling. For leaves, 4–6-months-old non-bearing twigs were sampled from 20 trees per orchard at breast height. Results showed that soil pH (7.1–8.4) was slightly alkaline, electrical conductivity (EC) was non-saline (<4 dSm−1), soil organic matter (SOM) was deficient (<0.86%), and calcium carbonate (CaCO3) was slight calcareous (<8%), at 0–15, 15–30, and 30–45 cm depths. The majority of soil samples were low in nitrogen (N) contents at all depths, i.e., (<0.043) 0–15 (85%), 15–30 (97%), and 30–45 (100%) cm depths. Phosphorus (P) was medium (7–15 mg kg−1) at 0–15 cm (60%) but low (<7 mg kg−1) at 15–30 (63%) and 30–45 cm (82%) depths. Potassium (K) was medium (80–180 mg kg−1) at 0–15 (69%), 15–30 (69%), and 30–45 cm (10%) depths. Boron (B) and manganese (Mn) were medium, and Cu was high in 0.15 cm, but all were low at 15–30 and 30–45 cm depths. Iron (Fe) and zinc (Zn) were low at depths of 0–15, 15–30, and 30–45 cm. Most citrus leaves were deficient in N (94%), Fe (76%), Zn (67%), and B (67%). In conclusion, soil fertilization is not sufficient for optimum citrus yield because of alkaline pH and slight calcareous soil conditions in this region. Foliar application of nutrients is suggested instead of only soil fertilization, for better nutrient management in citrus orchards.
Identification and Functional Characterization of Apple MdCKX5.2 in Root Development and Abiotic Stress Tolerance
Authors: Liu Yang , Wang Xun , Wang Xiaofei , Gao Wensheng , You Chunxiang
Published: January 2022

Cytokinin oxidase/dehydrogenases (CKXs) are the key enzymes in cytokinin degradation and have been widely studied in model plants. Little is known about apple’s (Malus×domestica) CKX genes. Here, using genome-wide analysis, we identified 10 MdCKX genes in apple. The phylogenetics, chromosome locations, and genome structures were then tested. Expression analysis showed that MdCKX genes had different expression profiles in apple, pointing to the different roles. Meanwhile, relative expression analysis showed that these genes have different expression patterns in response to several exogenous cytokinin factors, including trans-zeatin (ZT), thidiazuron (TDZ), and N6-furfuryladenine (KT). Finally, we introduced the MdCKX5.2 gene into Arabidopsis to evaluate its functions, and the results suggested the transgenic Arabidopsis displayed phenotypes related to promoting primary root and lateral root development, response to exogenous ZT, and conferring to drought and salt tolerant. Taken together, our results provide insights on the possible application of the MdCKX5.2 gene for molecular breeding in apples.
Loco-Regional Treatments for Hepatocellular Carcinoma in People Living with HIV
Authors: Micali Cristina , Russotto Ylenia , Caci Grazia , Ceccarelli Manuela , Marino Andrea , Celesia Benedetto Maurizio , Pellicanò Giovanni Francesco , Nunnari Giuseppe , Venanzi Rullo Emmanuele
Published: January 2022

Hepatocellular carcinoma (HCC) accounts for approximately 75–90% of primary liver cancers and is the sixth most common cancer and the third leading cause of cancer-related deaths worldwide. In the HIV-positive population, the risk of HCC is approximately four times higher than in the general population, with higher cancer-specific mortality than in HIV-negative patients. In most cases, HCC diagnosis is made in patients younger than the HIV-negative population and in the intermediate-advanced stage, thus limiting the therapeutic possibilities. Treatment choice in HIV-positive patients with HCC is subject to cancer staging, liver function and health status, as for HIV-negative and non-HIV-negative HCC patients. There are relatively few studies on the efficacy and safety in HIV-positive patients to date in loco-regional treatments for HCC. So far, literature shows that curative treatments such as radiofrequency ablation (RFA) have no significant differences in overall survival between HIV-positive and HIV-negative patients, as opposed to palliative treatments such as TACE, where there is a significant difference in overall survival. Although it can be assumed that the most recently discovered loco-regional therapies are applicable to HIV-positive patients with HCC in the same way as HIV-negative patients, further studies are needed to confirm this hypothesis. The purpose of our review is to evaluate these treatments, their efficacy, effectiveness, safety and their applicability to HIV-positive patients.
Impact of Nurse–Physician Collaboration, Moral Distress, and Professional Autonomy on Job Satisfaction among Nurses Acting as Physician Assistants
Authors: Kim Yunmi , Oh Younjae , Lee Eunhee , Kim Shin-Jeong
Published: January 2022

Although there is considerable literature on job satisfaction among nurses in various settings, there is little research about contributing factors, including moral distress to job satisfaction among a certain group of nurses, such as nurses acting as physician assistants. The purpose of this study was to verify the impact of nurse–physician collaboration, moral distress, and professional autonomy on job satisfaction among nurses acting as physician assistants. Descriptive and correlational research was conducted on a convenience sample of 130 nurses from five general hospitals in South Korea. In the final regression model, the adjusted R square was significant, explaining 38.2% of the variance of job satisfaction (F = 8.303, p < 0.001), where ‘cooperativeness’ (β = 0.469, p = 0.001) from nurse–physician collaboration, ‘institutional and contextual factor’ from moral distress (β = −0.292, p = 0.014), and professional autonomy (β = 0.247, p = 0.015) were included. In hospital environments, a more cooperative inter-professional relationship between nurses and physicians led to less moral distress caused by organisational constraints. A higher level of professional autonomy among nurses acting as physician assistants is required to increase their job satisfaction.
Exploring European Heavy Goods Vehicle Crashes Using a Three-Level Analysis of Crash Data
Authors: Schindler Ron , Jänsch Michael , Bálint András , Johannsen Heiko
Published: January 2022

Heavy goods vehicles (HGVs) are involved in 4.5% of police-reported road crashes in Europe and 14.2% of fatal road crashes. Active and passive safety systems can help to prevent crashes or mitigate the consequences but need detailed scenarios based on analysis of region-specific data to be designed effectively; however, a sufficiently detailed overview focusing on long-haul trucks is not available for Europe. The aim of this paper is to give a comprehensive and up-to-date analysis of crashes in the European Union that involve HGVs weighing 16 tons or more (16 t+). The identification of the most critical scenarios and their characteristics is based on a three-level analysis, as follows. Crash statistics based on data from the Community Database on Accidents on the Roads in Europe (CARE) provide a general overview of crashes involving HGVs. These results are complemented by a more detailed characterization of crashes involving 16 t+ trucks based on national road crash data from Italy, Spain, and Sweden. This analysis is further refined by a detailed study of crashes involving 16 t+ trucks in the German In-Depth Accident Study (GIDAS), including a crash causation analysis. The results show that most European HGV crashes occur in clear weather, during daylight, on dry roads, outside city limits, and on nonhighway roads. Three main scenarios for 16 t+ trucks are characterized in-depth: rear-end crashes in which the truck is the striking partner, conflicts during right turn maneuvers of the truck with a cyclist riding alongside, and pedestrians crossing the road in front of the truck. Among truck-related crash causes, information admission failures (e.g., distraction) were the main crash causation factor in 72% of cases in the rear-end striking scenario while information access problems (e.g., blind spots) were present for 72% of cases in the cyclist scenario and 75% of cases in the pedestrian scenario. The three levels of data analysis used in this paper give a deeper understanding of European HGV crashes, in terms of the most common crash characteristics on EU level and very detailed descriptions of both kinematic parameters and crash causation factors for the above scenarios. The results thereby provide both a global overview and sufficient depth of analysis of the most relevant cases and aid safety system development.
Gender-Specific Determinants of eHealth Literacy: Results from an Adolescent Internet Behavior Survey in Taiwan
Authors: Cheng Chia-Shiang , Huang Yi-Jen , Sun Chien-An , An Chi , Chang Yu-Tien , Chu Chi-Ming , Chang Chi-Wen
Published: January 2022

Adolescents’ Internet health information usage has rarely been investigated. Adolescents seek all kinds of information from the Internet, including health information, which affects their Health Literacy that eHealth Literacy (eHL). This study is a retrospective observational study, we have total of 500 questionnaires were distributed, 87% of which were recovered, and we explored the channels that adolescents use to search for health information, their ability to identify false information, and factors affecting the type and content of health information queried. Adolescents believe that the Internet is a good means to seek health information because of its instant accessibility, frequent updating, convenience, and lack of time limits. More boys use the Internet to seek health information than girls in junior high schools (p = 0.009). The Internet is an important source of health information for adolescents but contains extensive misinformation that adolescents cannot identify. Additionally, adolescent boys and girls are interested in different health issues. Therefore, the government should implement measures to minimize misinformation on the Internet and create a healthy, educational online environment to promote Adolescents’ eHealth Literacy (eHL).
Territorial Strategy of Medical Units for Addressing the First Wave of the COVID-19 Pandemic in the Metropolitan Area of Mexico City: Analysis of Mobility, Accessibility and Marginalization
Authors: Galindo-Pérez Mateo Carlos , Suárez Manuel , Rosales-Tapia Ana Rosa , Sifuentes-Osornio José , Angulo-Guerrero Ofelia , Benítez-Pérez Héctor , de Anda-Jauregui Guillermo , Díaz-de-León-Santiago Juan Luis , Hernández-Lemus Enrique , Alonso Herrera Luis , López-Arellano Oliva , Revuelta-Herrera Arturo , Ruiz-Gutiérrez Rosaura , Sheinbaum-Pardo Claudia , Kershenobich-Stalnikowitz David
Published: January 2022

Background. The COVID-19 pandemic has caused an exponential increase in the demand for medical care worldwide. In Mexico, the COVID Medical Units (CMUs) conversion strategy was implemented. Objective. To evaluate the CMU coverage strategy in the Mexico City Metropolitan Area (MCMA) by territory. Materials. The CMU directory was used, as were COVID-19 infection and mobility statistics and Mexican 2020 census information at the urban geographic area scale. The degree of urban marginalization by geographic area was also considered. Method. Using descriptive statistics and the calculation of a CMU accessibility index, population aggregates were counted based on coverage radii. In addition, two regression models are proposed to explain (1) the territorial and temporal trend of COVID-19 infections in the MCMA and (2) the mobility of the COVID-infected population visiting medical units. Results. The findings of the evaluation of the CMU strategy were (1) in the MCMA, COVID-19 followed a pattern of contagion from the urban center to the periphery; (2) given the growth in the number of cases and the overload of medical units, the population traveled greater distances to seek medical care; (3) after the CMU strategy was evaluated at the territory level, it was found that 9 out of 10 inhabitants had a CMU located approximately 7 km away; and (4) at the metropolitan level, the lowest level of accessibility to the CMU was recorded for the population with the highest levels of marginalization, i.e., those residing in the urban periphery.
Predictive Model of Quality of Life in Patients with Parkinson’s Disease
Authors: Candel-Parra Eduardo , Córcoles-Jiménez María Pilar , Delicado-Useros Victoria , Ruiz-Grao Marta Carolina , Hernández-Martínez Antonio , Molina-Alarcón Milagros
Published: January 2022

Parkinson’s disease is a chronic, progressive, and disabling neurodegenerative disease which evolves until the end of life and triggers different mood and organic alterations that influence health-related quality of life. The objective of our study was to identify the factors that negatively impact the quality of life of patients with Parkinson’s disease and construct a predictive model of health-related quality of life in these patients. Methods: An analytical, prospective observational study was carried out, including Parkinson’s patients at different stages in the Albacete Health Area. The sample consisted of 155 patients (T0) who were followed up at one (T1) and two years (T2). The instruments used were a purpose-designed data collection questionnaire and the “Parkinson’s Disease Questionnaire” (PDQ-39), with a global index where a higher score indicates a worse quality of life. A multivariate analysis was performed by multiple linear regression at T0. Next, the model’s predictive capacity was evaluated at T1 and T2 using the area under the ROC curve (AUROC). Results: Predictive factors were: sex, living in a residence, using a cane, using a wheelchair, having a Parkinson’s stage of HY > 2, having Alzheimer’s disease or a major neurocognitive disorder, having more than five non-motor symptoms, polypharmacy, and disability greater than 66%. This model showed good predictive capacity at one year and two years of follow-up, with an AUROC of 0.89 (95% CI: 0.83–0.94) and 0.83 (95% CI: 0.76–0.89), respectively. Conclusions: A predictive model constructed with nine variables showed a good discriminative capacity to predict the quality of life of patients with Parkinson’s disease at one and two years of follow-up.
Responsiveness and Adaptability of Healthcare Facilities in Emergency Scenarios: COVID-19 Experience
Authors: Łukasik Marta , Porębska Anna
Published: January 2022

The COVID-19 pandemic revealed many vulnerabilities of the contemporary built environment along with limited preparedness and low efficiency in mitigating unexpected and unprecedented challenges. This article discusses the efficiency and responsiveness of basic hospital spatial layouts in three different scenarios: normal operation; the segregation of a large number of patients and still providing them with access to emergency healthcare, typical for a pandemic; and a sudden, extremely high number of admissions typical for compound disasters and terrorist attacks. A set of parameters and a method for general adaptability assessment (GAAT) that can be used as a tool in decision-making processes as well as evaluation of both existing facilities and the new models for resilient hospitals resulting from the experience of the pandemic are proposed. The paper emphasizes why factors among which adaptability, convertibility, and scalability should be at the very core of hospital development and management strategies. It also discusses new models of adaptable healthcare facilities that enable day-to-day operations to continue alongside a pandemic, and other emergency scenarios.
Hyperthermic Intraperitoneal Chemotherapy in the Management of Gastric Cancer: A Narrative Review
Authors: Mazurek Marek , Szlendak Małgorzata , Forma Alicja , Baj Jacek , Maciejewski Ryszard , Roviello Giandomenico , Marano Luigi , Roviello Franco , Polom Karol , Sitarz Robert
Published: January 2022

Gastric cancer (GC) patients with peritoneal metastasis tend to achieve poor clinical outcomes. Until recently, the treatment options were limited mainly to either palliative chemotherapy or radiation therapy in exceptional cases. Currently, these patients benefit from multimodal treatment, such as cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC). Despite good overall results, this treatment modality is still widely debated. The following study is designed to assess the papers about the possible application and utility of HIPEC in GC. A search in the PubMed, Web of Science, and Scopus databases was performed to assess the papers devoted to the role of HIPEC in GC treatment; a literature search was performed until March 21st; and, finally, 50 studies with a total number of 3946 patients were analyzed. According to the most recent data, it seems to be reasonable to limit the duration of HIPEC to the shortest effective time. Moreover, the drugs used in HIPEC need to have equal concentrations and the same solvent. Perioperative chemotherapy needs to be reported in detail and, furthermore, the term “morbidity” should be defined more clearly by the authors.
Complex Contact Network of Patients at the Beginning of an Epidemic Outbreak: An Analysis Based on 1218 COVID-19 Cases in China
Authors: Yang Zhangbo , Zhang Jiahao , Gao Shanxing , Wang Hui
Published: January 2022

The spread of viruses essentially occurs through the interaction and contact between people, which is closely related to the network of interpersonal relationships. Based on the epidemiological investigations of 1218 COVID-19 cases in eight areas of China, we use text analysis, social network analysis and visualization methods to construct a dynamic contact network of the epidemic. We analyze the corresponding demographic characteristics, network indicators, and structural characteristics of this network. We found that more than 65% of cases are likely to be infected by a strong relationship, and nearly 40% of cases have family members infected at the same time. The overall connectivity of the contact network is low, but there are still some clustered infections. In terms of the degree distribution, most cases’ degrees are concentrated between 0 and 2, which is relatively low, and only a few ones have a higher degree value. The degree distribution also conforms to the power law distribution, indicating the network is a scale-free network. There are 17 cases with a degree greater than 10, and these cluster infections are usually caused by local transmission. The first implication of this research is we find that the COVID-19 spread is closely related to social structures by applying computational sociological methods for infectious disease studies; the second implication is to confirm that text analysis can quickly visualize the spread trajectory at the beginning of an epidemic.
Association between Total and Individual PCB Congener Levels in Maternal Serum and Birth Weight of Newborns: Results from the Chiba Study of Mother and Child Health Using Weighted Quantile Sum Regression
Authors: Eguchi Akifumi , Sakurai Kenichi , Yamamoto Midori , Watanabe Masahiro , Hisada Aya , Takahashi Tomoko , Todaka Emiko , Mori Chisato
Published: January 2022

Maternal exposure to polychlorinated biphenyls (PCBs) during pregnancy is associated with a low birth weight; however, the congener-specific effects of PCB congeners are not well defined. In this study, we used maternal serum samples from the Chiba Study of Mother and Child Health (C-MACH) cohort, collected at 32 weeks of gestational age, to analyze the effects of PCB congener exposure on birth weight by examining the relationship between newborn birth weight and individual PCB congener levels in maternal serum (n = 291). The median total PCB level in the serum of mothers of male and female newborns at approximately 32 weeks of gestation was 39 and 37 ng g−1 lipid wt, respectively. The effect of the total PCB levels and the effects of PCB congener mixtures were analyzed using a linear regression model and a generalized weighted quantile sum regression model (gWQS). The birth weight of newborns was significantly associated with maternal exposure to PCB mixtures in the gWQS model. The results suggest that exposure to PCB mixtures results in low newborn birth weight. However, specific impacts of individual PCB congeners could not be related to newborn birth weight.
Tracking of Maternal Physical Activity and Sport Participation over 11 Years: Findings from the Czech ELSPAC Study
Authors: Kasović Mario , Štefan Lovro , Piler Pavel , Zvonar Martin
Published: January 2022

Purpose: Tracking of physical activity (PA) and sport participation (SP) during motherhood is poorly understood. The purpose of the study was to analyze the extent of tracking of maternal PA and SP. Methods: In this investigation, data were collected from the Czech ELSPAC study subsample of 4811 and 2609 women measured postnatally (1991–1992) and after 11 years of follow-up (2002–2003), respectively. The structured questionnaire was used to assess the participation and average weekly time spent in PA, and the frequency of engaging in different sports (running, cycling, strength training, racket sports, swimming, and team sports). Tracking was calculated using generalized estimating equations (GEE) with beta coefficients (β), odds ratios (ORs), and 95% confidence intervals (95% CI). Results: Moderately high tracking coefficients were observed for cycling (β = 0.69, 95% 0.67–0.72), strength training (β = 0.59, 95% 0.56–0.63), and weekly time spent in PA (β = 0.53, 95% 0.38–0.66); meanwhile, moderate tracking coefficients were generated for swimming (β = 0.48, 95% 0.44–0.52), team sports (β = 0.44, 95% 0.39–0.48), racket sports (β = 0.44, 95% 0.39–0.48), and running (β = 0.35, 95% 0.30–0.40). Mothers who did not participate in PA at baseline were 81% more likely not to participate in it at follow-up (OR = 1.81, 95% CI 1.53–2.13). Conclusion: Cycling- and strength-related activities and weekly PA were tracked moderately-to-moderately high during motherhood. Moreover, the strong tracking of physical inactivity indicates that the detection of this risk factor before pregnancy should be advocated.
Post-Exposure Prophylaxis and Methamphetamine Use among Young Sexual Minority Men: The P18 Cohort Study
Authors: Kaplun Elizabeth , Martino Richard , Krause Kristen , Briganti Michael , D’Avanzo Paul , Halkitis Perry
Published: January 2022

Methamphetamine use is associated with increased risk of HIV infection among young sexual minority men (SMM). Post-exposure prophylaxis (PEP) is an effective strategy for individuals who are exposed to HIV, but there is limited research about PEP use among young SMM and its relationship with methamphetamine use. This study analyzes the association between ever PEP use and recent methamphetamine use among young SMM in New York City, using cross-sectional data from the P18 Cohort Study (n = 429). Multivariable logistic regression models were used to assess the association between methamphetamine use and ever PEP use. Compared with those who had not used methamphetamine in the last 6 months, young SMM who did use methamphetamine were significantly more likely to have ever used PEP (AOR = 6.07, 95% CI: 2.10–16.86). Young SMM who had ever used PrEP had 16 times higher odds of ever using PEP (AOR = 16, 95% CI: 7.41–35.95). Those who completed bachelor’s degrees were 61% less likely to have ever used PEP (AOR = 0.39, 95% CI: 0.17–0.88). These data suggest that methamphetamine use could increase the risk of HIV infection, highlighting the critical need to target interventions for young SMM who use methamphetamine and are more likely to engage in unprotected intercourse.
Comparison of the Cardiovascular Effects of Extreme Psychological and Physical Stress Tests in Male Soccer Players
Authors: Móra Ákos , Komka Zsolt , Végh József , Farkas István , Kocsisné Gyöngyi Szilágyi , Bosnyák Edit , Szmodis Márta , Ligetvári Roland , Csöndör Éva , Almási Gábor , Oláh András , Kemper Han C. G. , Tóth Miklós , Ács Pongrác
Published: January 2022

Background: The purpose of our study was to compare the physiological effects of extreme physical and psychological stress tests in male soccer players, since these two types of stress apply to athletes with high performance requirements. Methods: A total of 63 healthy male soccer players participated in this study, all of whom underwent both of the tests. A physical stress test was carried out in an exercise physiology laboratory, where subjects completed an incremental treadmill running test to full exhaustion, and a psychological test was performed in a military tactical room, where subjects met a street offence situation. Heart rate variability (HRV) and blood pressure (BP) were recorded directly before, immediately after, and 30 min after the stress tests. Results: The majority of HRV indices changed significantly in both stress protocols. Inverse, significant changes (positive for the physical test, negative for the psychological test, p < 0.001) were found when comparing the alterations of HRV indices between the tests. Significant differences were found in the changes in systolic (p = 0.003) and diastolic (p < 0.001) BP between the test protocols, and also between the baseline and post-test measurements (p < 0.001). Conclusion: Both HRV and BP are sensitive physiological parameters to measure the impact of extreme physical and/or psychological stress
An Overview of Environmental Risk Factors for Food Allergy
Authors: Peters Rachel L. , Mavoa Suzanne , Koplin Jennifer J.
Published: January 2022

IgE-mediated food allergy is an increasing public health concern in many regions around the world. Although genetics play a role in the development of food allergy, the reported increase has occurred largely within a single generation and therefore it is unlikely that this can be accounted for by changes in the human genome. Environmental factors must play a key role. While there is strong evidence to support the early introduction of allergenic solids to prevent food allergy, this is unlikely to be sufficient to prevent all food allergy. The purpose of this review is to summarize the evidence on risk factors for food allergy with a focus the outdoor physical environment. We discuss emerging evidence of mechanisms that could explain a role for vitamin D, air pollution, environmental greenness, and pollen exposure in the development of food allergy. We also describe the recent extension of the dual allergen exposure hypothesis to potentially include the respiratory epithelial barrier in addition to the skin. Few existing studies have examined the relationship between these environmental factors with objective measures of IgE-mediated food allergy and further research in this area is needed. Future research also needs to consider the complex interplay between multiple environmental factors.
The Impact of Green Technology Innovation on Carbon Emissions in the Context of Carbon Neutrality in China: Evidence from Spatial Spillover and Nonlinear Effect Analysis
Authors: Zeng Shihong , Li Gen , Wu Shaomin , Dong Zhanfeng
Published: January 2022

The Paris agreement is a unified arrangement for the global response to climate change and entered into force on 4 November 2016. Its long-term goal is to hold the global average temperature rise well below 2 °C. China is committed to achieving carbon neutrality by 2060 through various measures, one of which is green technology innovation (GTI). This paper aims to analyze the levels of GTI in 30 provinces in mainland China between 2001 and 2019. It uses the spatial econometric models and panel threshold models along with the slack based measure (SBM) and Global Malmquist-Luenberger (GML) index to analyze the spatial spillover and nonlinear effects of GTI on regional carbon emissions. The results show that GTI achieves growth every year, but the innovation efficiency was low. China’s total carbon dioxide emissions were increasing at a marginal rate, but the carbon emission intensity was declining year by year. Carbon emissions were spatially correlated and show significant positive agglomeration characteristics. The spatial spillover of GTI plays an important role in reducing carbon dioxide emissions. In the underdeveloped regions in China, this emission reduction effect was even more significant.
Effects of Personal Low-Frequency Stimulation Device on Myalgia: A Randomized Controlled Trial
Authors: Yoon Yong-Soon , Ko Myoung-Hwan , Cho Il-Young , Kim Cheol-Su , Bajgai Johny , Jang Hong-Young , Kim Ka-Eun , Lee Kyu-Jae , Lee Mihyun
Published: January 2022

Electrotherapy is commonly used for myalgia alleviation. Low-frequency stimulation (LFS) is primarily used for controlling acute and chronic pain and is a non-invasive therapy that can be easily performed with electric stimulation applied on the skin. However, little evidence exists regarding the pain alleviation effects of personal low-frequency stimulation device for home use. Moreover, no studies have compared myalgia alleviation effects between personal low-frequency stimulation (PLS) and physical therapy (PT), which are most commonly used for patients with myalgia in hospitals and clinics. Therefore, we aimed to investigate the pain alleviation effects of PLS in patients with myalgia and compare these effects with those of conventional PT (transcutaneous electrical nerve stimulation + ultrasound). In total, 39 patients with myalgia in the neck, shoulder, back, and waist areas were randomly assigned to the personal low-frequency stimulation group (PLSG: n = 20) and physical therapy group (PTG: n = 19). Both groups were treated for 3 weeks (20 min per session and 5 sessions per week). Patients were assessed for pain intensity by surface electromyography (sEMG), visual analogue scale (VAS) and a short-form McGill pain questionnaire (SF-MPQ) before and after the intervention period. Our results showed that PLSG showed a tendency of muscle relaxation with a significant decrease in sEMG in the neck (p = 0.0425), shoulder (p = 0.0425), and back (p = 0.0046) areas compared to the control group. However, there was no significant difference in waist area. Additionally, VAS scores significantly decreased between pre- and post-treatment in both PTG (p = 0.0098), and PLSG (p = 0.0304) groups, but there was no significance difference between the groups. With respect to SF-MPQ, the PLSG showed greater pain alleviation (5.23 ± 0.25) effects than the PTG (6.23 ± 0.25). Accordingly, our results suggest that PLS treatment using a home device might offer positive assistance in pain alleviation for patients with myalgia that is as equally effective as conventional PT treatment. However, further detailed studies are required considering larger samples to fully claim the effectiveness of this device.
Multi-Regional Modeling of Cumulative COVID-19 Cases Integrated with Environmental Forest Knowledge Estimation: A Deep Learning Ensemble Approach
Authors: Alamrouni Abdelgader , Aslanova Fidan , Mati Sagiru , Maccido Hamza Sabo , Jibril Afaf. A. , Usman A. G. , Abba S. I.
Published: January 2022

Reliable modeling of novel commutative cases of COVID-19 (CCC) is essential for determining hospitalization needs and providing the benchmark for health-related policies. The current study proposes multi-regional modeling of CCC cases for the first scenario using autoregressive integrated moving average (ARIMA) based on automatic routines (AUTOARIMA), ARIMA with maximum likelihood (ARIMAML), and ARIMA with generalized least squares method (ARIMAGLS) and ensembled (ARIMAML-ARIMAGLS). Subsequently, different deep learning (DL) models viz: long short-term memory (LSTM), random forest (RF), and ensemble learning (EML) were applied to the second scenario to predict the effect of forest knowledge (FK) during the COVID-19 pandemic. For this purpose, augmented Dickey–Fuller (ADF) and Phillips–Perron (PP) unit root tests, autocorrelation function (ACF), partial autocorrelation function (PACF), Schwarz information criterion (SIC), and residual diagnostics were considered in determining the best ARIMA model for cumulative COVID-19 cases (CCC) across multi-region countries. Seven different performance criteria were used to evaluate the accuracy of the models. The obtained results justified both types of ARIMA model, with ARIMAGLS and ensemble ARIMA demonstrating superiority to the other models. Among the DL models analyzed, LSTM-M1 emerged as the best and most reliable estimation model, with both RF and LSTM attaining more than 80% prediction accuracy. While the EML of the DL proved merit with 96% accuracy. The outcomes of the two scenarios indicate the superiority of ARIMA time series and DL models in further decision making for FK.
Ecologies of Resilience for Australian High School Students from Refugee Backgrounds: Quantitative Study
Authors: Miller Emily , Ziaian Tahereh , de Anstiss Helena , Baak Melanie
Published: January 2022

Forced displacement of refugees, currently at record levels, leads to increased cultural diversity in many countries with benefits and challenges for individuals, communities, and societies. Refugees often face significant stressors both pre- and post-migration, and hence are at increased risk of poor mental health and wellbeing. Children and adolescents make up a significant proportion of refugees globally, and hence mental health supports for these young people are crucial. Current mental health research often uses pathologized approaches that focus on trauma, although there is growing literature highlighting the importance of a sense of belonging and the reduction in discrimination and social exclusion, emphasizing strengths and agency of individuals and communities. Resilience is often noted for its positive influence on mental health and wellbeing; however, research regarding how mechanisms of resilience function is still developing. This study investigated mental health and wellbeing of refugee-background Australian youth to better understand the role and function of resilience. Findings suggest that intersecting social ecologies, such as those within family, school, or community networks, contribute to development of identity and a sense of belonging for youth, which together form a resilient system that provides resources for wellbeing. Adaptations of school policy and practice can support positive mental health and wellbeing outcomes by contributing to and developing resilient environments, such as through building connections to family, improving positive recognition of cultural identity for individuals and across the whole school community, and actively working to minimize discrimination.
The Effects of Traffic Air Pollution in and around Schools on Executive Function and Academic Performance in Children: A Rapid Review
Authors: Gartland Nicola , Aljofi Halah E. , Dienes Kimberly , Munford Luke Aaron , Theakston Anna L. , van Tongeren Martie
Published: January 2022

This review summarises the extant literature investigating the relation between traffic-related air pollution levels in and around schools and executive functioning in primary-school-aged children. An electronic search was conducted using Web of Science, Scopus, and Education Literature Datasets databases (February 2020). Review articles were also searched, and forwards and backwards searches of identified studies were performed. Included papers were assessed for quality. We included 9 separate studies (published in 13 papers). Findings suggest that indoor and outdoor particulate matter with a diameter of 2.5 μm or less (PM2.5) negatively influences executive function and academic achievement and that indoor and outdoor nitrogen dioxide (NO2) adversely affects working memory. Evidence for the effects of particulate matter with a diameter of 10 μm or less (PM10) is limited but suggests potential wide-ranging negative effects on attention, reasoning, and academic test scores. Air pollution in and around schools influences executive function and appears to impede the developmental trajectory of working memory. Further research is required to establish the extent of these effects, reproducibility, consequences for future attainment, and place within the wider context of cognitive development.
Do School-Level Factors Affect the Health Behaviors of High School Students in Korea?
Authors: Kwak Seon-Hui , Lee Hyo-Jin , Shin Bo-Mi
Published: January 2022

We conducted a multilevel analysis to identify the individual- and school-level factors that affect Korean high school students’ tooth brushing, soda intake, smoking, and high-intensity physical activity. We sampled 27,919 high school students from the 15th Korea Youth Risk Behavior Web-Based Survey. The individual-level variables included demographic, socioeconomic, and health-related factors. The school-level variables included school system and school type. Regarding the individual-level factors, economic level and academic performance had a significant effect on health behavior when the demographic variables were adjusted. In the final model, the school-level factors had a significant effect on health behavior. The odds ratio (OR) of brushing less than twice a day in vocational schools compared to general schools was 1.63 (p < 0.001), and the OR of soda intake more than three times a week in vocational schools was 1.33 (p < 0.001). In addition, the OR of smoking in vocational schools was 2.89 (p < 0.001), and the OR of high-intensity physical activity in vocational schools was 0.80 (p < 0.001). Therefore, both individual- and school-level factors affect Korean students’ health behaviors. A school-based comprehensive health promotion strategy should be developed that considers schools’ characteristics to equip all students with health awareness, regardless of socioeconomic status.
Sirtuins Modulation: A Promising Strategy for HIV-Associated Neurocognitive Impairments
Authors: Figarola-Centurión Izchel , Escoto-Delgadillo Martha , González-Enríquez Gracia Viviana , Gutiérrez-Sevilla Juan Ernesto , Vázquez-Valls Eduardo , Torres-Mendoza Blanca Miriam
Published: January 2022

HIV-Associated neurocognitive disorder (HAND) is one of the major concerns since it persists in 40% of this population. Nowadays, HAND neuropathogenesis is considered to be caused by the infected cells that cross the brain–blood barrier and produce viral proteins that can be secreted and internalized into neurons leading to disruption of cellular processes. The evidence points to viral proteins such as Tat as the causal agent for neuronal alteration and thus HAND. The hallmarks in Tat-induced neurodegeneration are endoplasmic reticulum stress and mitochondrial dysfunction. Sirtuins (SIRTs) are NAD+-dependent deacetylases involved in mitochondria biogenesis, unfolded protein response, and intrinsic apoptosis pathway. Tat interaction with these deacetylases causes inhibition of SIRT1 and SIRT3. Studies revealed that SIRTs activation promotes neuroprotection in neurodegenerative diseases such Alzheimer’s and Parkinson’s disease. Therefore, this review focuses on Tat-induced neurotoxicity mechanisms that involve SIRTs as key regulators and their modulation as a therapeutic strategy for tackling HAND and thereby improving the quality of life of people living with HIV.