bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal trends in the incidence, treatment patterns, and outcomes of coronary artery disease and peripheral artery disease in the UK, 2006–2015
Authors: Sundaram Varun , Bloom Chloe , Zakeri Rosita , Halcox Julian , Cohen Alexander , Bowrin Kevin , Briere Jean-Baptiste , Banerjee Amitava , Simon Daniel I , Cleland John G F , Rajagopalan Sanjay , Quint Jennifer K
Published: December 2019

Aims

Most reports estimating national incidence rates of coronary (CAD) and peripheral arterial disease (PAD) have focused on stable outpatients or acute or elective hospital admissions, but not on the overall burden of disease. In this study, we report the changing trends in the population-level incidence of CAD and PAD, respectively from 2006 to 2015, statin utilization for secondary prevention and survival outcomes using multiple nationally representative data sources from the UK (primary care encounters, hospital admissions, and procedure-level data).

Methods and results

A nationally representative study of linked primary and secondary care electronic health records of 4.6 million individuals from the UK. We calculated crude and standardized annual incidence rates separately for CAD and PAD. Statin use for secondary prevention, trends in annual major vascular event rates, and mortality between 2006 and 2015, were estimated for CAD and PAD, respectively. We identified 160 376 and 70 753 patients with incident CAD and PAD, respectively. The age- and sex-standardized incidence of CAD was similar in 2006 (443 per 100 000 person-years) and 2015 [436 per 100 000 person-years; adjusted incidence rate ratio (IRR) 0.98, 95% confidence interval (CI) 0.96–1.00]. In contrast, there was a 15% decline in the standardized incidence of PAD (236 per 100 000 person-years in 2006 to 202 per 100 000 person-years in 2015; adjusted IRR 0.85, 95% CI 0.82–0.88). The proportion of incident CAD and PAD patients prescribed long-term statins, was only 66% and 55%, respectively and was less common amongst women, patients aged >70 years, with heart failure, chronic lung disease, or depression. Cardiovascular mortality declined by 43% for incident CAD (adjusted IRR 0.57, 95% CI 0.50–0.64) between 2006 and 2015 but did not decline for incident PAD (adjusted IRR 0.84, 95% CI 0.70–1.00).

Conclusion and relevance

In the UK, the standardized incidence of CAD appears stable but mortality rates are falling, whereas the standardized incidence of PAD is falling but mortality rates are not.
Risk of hypertension into adulthood in persons born prematurely: a national cohort study
Authors: Crump Casey , Sundquist Jan , Sundquist Kristina
Published: December 2019

Aims

Preterm birth has been associated with elevated blood pressure early in life; however, hypertension risks from childhood into adulthood remain unclear. We conducted a large population-based study to examine gestational age at birth in relation to hypertension risks from childhood into adulthood.

Methods and results

A national cohort study was conducted of all 4 193 069 singleton live births in Sweden during 1973–2014, who were followed up for hypertension identified from nationwide inpatient and outpatient (specialty and primary care) diagnoses from any health care encounters through 2015 (maximum age 43 years; median 22.5). Cox regression was used to examine gestational age at birth in relation to hypertension risk while adjusting for other perinatal and maternal factors, and co-sibling analyses assessed the potential influence of unmeasured shared familial (genetic and/or environmental) factors. In 86.8 million person-years of follow-up, 62 424 (1.5%) persons were identified with hypertension (median age 29.8 years at diagnosis). Adjusted hazard ratios for new-onset hypertension at ages 18–29 years associated with preterm (<37 weeks) and extremely preterm (22–27 weeks) birth were 1.28 [95% confidence interval (CI), 1.21–1.36] and 2.45 (1.82–3.31), respectively, and at ages 30–43 years were 1.25 (1.18–1.31) and 1.68 (1.12–2.53), respectively, compared with full-term birth (39–41 weeks). These associations affected males and females similarly and appeared substantially related to shared genetic or environmental factors in families.

Conclusions

In this large national cohort, preterm birth was associated with increased risk of hypertension into early adulthood. Persons born prematurely may need early preventive evaluation and long-term monitoring for the development of hypertension.
Magnetic resonance imaging in non-conditional pacemakers and implantable cardioverter-defibrillators: a systematic review and meta-analysis
Authors: Munawar Dian A , Chan Joel E Z , Emami Mehrdad , Kadhim Kadhim , Khokhar Kashif , O’Shea Catherine , Iwai Shinsuke , Pitman Bradley , Linz Dominik , Munawar Muhammad , Roberts-Thomson Kurt , Young Glenn D , Mahajan Rajiv , Sanders Prashanthan , Lau Dennis H
Published: December 2019

Aims

There is growing evidence that magnetic resonance imaging (MRI) scanning in patients with non-conditional cardiac implantable electronic devices (CIEDs) can be performed safely. Here, we aim to assess the safety of MRI in patients with non-conditional CIEDs.

Methods and results

English scientific literature was searched using PubMed/Embase/CINAHL with keywords of ‘magnetic resonance imaging’, ‘pacemaker’, ‘implantable defibrillator’, and ‘cardiac resynchronization therapy’. Studies assessing outcomes of adverse events or significant changes in CIED parameters after MRI scanning in patients with non-conditional CIEDs were included. References were excluded if the MRI conditionality of the CIEDs was undisclosed; number of patients enrolled was <10; or studies were case reports/series. 35 cohort studies with a total of 5625 patients and 7196 MRI scans (0.5–3 T) in non-conditional CIEDs were included. The overall incidence of lead failure, electrical reset, arrhythmia, inappropriate pacing and symptoms related to pocket heating, or torque ranged between 0% and 1.43%. Increase in pacing lead threshold >0.5 V and impedance >50Ω was seen in 1.1% [95% confidence interval (CI) 0.7–1.8%] and 4.8% (95% CI 3.3–6.4%) respectively. The incidence of reduction in P- and R-wave sensing by >50% was 1.5% (95% CI 0.6–2.9%) and 0.4% (95% CI 0.06–1.1%), respectively. Battery voltage reduction of >0.04 V was reported in 2.2% (95% CI 0.2–6.1%).

Conclusion

This meta-analysis affirms the safety of MR imaging in non-conditional CIEDs with no death or implantable cardioverter-defibrillator shocks and extremely low incidence of lead or device-related complications.
A novel application of small area estimation in loblolly pine forest inventory
Authors: Green P Corey , Burkhart Harold E , Coulston John W , Radtke Philip J
Published: December 2019

Loblolly pine (Pinus taeda L.) is one of the most widely planted tree species globally. As the reliability of estimating forest characteristics such as volume, biomass and carbon becomes more important, the necessary resources available for assessment are often insufficient to meet desired confidence levels. Small area estimation (SAE) methods were investigated for their potential to improve the precision of volume estimates in loblolly pine plantations aged 9–43. Area-level SAE models that included lidar height percentiles and stand thinning status as auxiliary information were developed to test whether precision gains could be achieved. Models that utilized both forms of auxiliary data provided larger gains in precision compared to using lidar alone. Unit-level SAE models were found to offer additional gains compared with area-level models in some cases; however, area-level models that incorporated both lidar and thinning status performed nearly as well or better. Despite their potential gains in precision, unit-level models are more difficult to apply in practice due to the need for highly accurate, spatially defined sample units and the inability to incorporate certain area-level covariates. The results of this study are of interest to those looking to reduce the uncertainty of stand parameter estimates. With improved estimate precision, managers, stakeholders and policy makers can have more confidence in resource assessments for informed decisions.
Emergence and Evolution of ERM Proteins and Merlin in Metazoans
Authors: Shabardina Victoria , Kashima Yukie , Suzuki Yutaka , Makalowski Wojciech
Published: December 2019

Ezrin, radixin, moesin, and merlin are cytoskeletal proteins, whose functions are specific to metazoans. They participate in cell cortex rearrangement, including cell–cell contact formation, and play an important role in cancer progression. Here, we have performed a comprehensive phylogenetic analysis of the proteins spanning 87 species. The results describe a possible mechanism for the protein family origin in the root of Metazoa, paralogs diversification in vertebrates, and acquisition of novel functions, including tumor suppression. In addition, a merlin paralog, present in most vertebrates but lost in mammals, has been described here for the first time. We have also highlighted a set of amino acid variations within the conserved motifs as the candidates for determining physiological differences between ERM paralogs.
Examining the ecological role of jellyfish in the Eastern Bering Sea
Authors: Ruzicka James , Brodeur Richard D , Cieciel Kristin , Decker Mary Beth
Published: December 2019

Within the Eastern Bering Sea, the jellyfish Chrysaora melanaster has fluctuated widely over recent decades. We examined the role of C. melanaster as an ecosystem-structuring agent via application of ecosystem models representing inner-, mid-, and outer-shelf regions of comparable areal coverage. Chrysaora melanaster utilize 1% of total mid-shelf consumer production, or 1/4th the energy required by forage fish (capelin Mallotus villosus, Pacific herring Clupea pallasii, age-0 Pacific cod Gadus macrocephalus, age-0 walleye pollock Gadus chalcogrammus). Model simulations show the impacts of C. melanaster are broadly distributed across consumer groups with increasingly negative impacts with higher jellyfish biomass. Age-0 pollock represent the greater part of the forage fish biomass, and observed pollock biomass during low jellyfish years (2004–2007) was significantly greater than during high jellyfish years (2009–2014). However, sensitivity among consumer groups to observed jellyfish variability is small, within 5% of baseline (2004–2015) conditions. Estimates using similar models for the Coastal Gulf of Alaska (CGoA) and Northern California Current (NCC) suggest large differences in the role of scyphozoans among northern Pacific shelf ecosystems. Only 0.1% of total summer consumer production is required to support CGoA Chrysaora, while the coastal NCC population uses 19%.
The influence of a lipid reservoir on the tear film formation
Authors: Maki Kara L , Braun Richard J , Barron Gregory A
Published: December 2019

We present a mathematical model to study the influence of a lipid reservoir, seen experimentally, at the lid margin on the formation and relaxation of the tear film during a partial blink. Applying the lubrication limit, we derive two coupled non-linear partial differential equations characterizing the evolution of the aqueous tear fluid and the covering insoluble lipid concentration. Departing from prior works, we explore a new set of boundary conditions (BCs) enforcing hypothesized lipid concentration dynamics at the lid margins. Using both numerical and analytical approaches, we find that the lipid-focused BCs strongly impact tear film formation and thinning rates. Specifically, during the upstroke of the eyelid, we find specifying the lipid concentration at the lid margin accelerates thinning. Parameter regimes that cause tear film formation success or failure are identified. More importantly, this work expands our understanding of the consequences of lipid dynamics near the lid margins for tear film formation.
Freedom of Religious Association: Towards a Purposive Interpretation of the Employment Equality Exceptions
Authors: Cannon Catriona
Published: December 2019

The exceptions to the principle of equality in employment for employers in Britain with a religious or belief ethos (or who employ personnel for the purposes of organised religion) are ambiguous in scope and lack any clear foundational principle to guide judicial interpretation. In view of the consequent risk of inconsistency in decision-making, this article addresses the question of how best to understand and interpret the exceptions. While the exceptions should be regarded as limited derogations from the equality principle, it is nonetheless important that recognition is afforded to their underlying rationale which, I argue, derives from the fundamental human rights of religious association. Though the concept of associative rights and related ideas have not featured heavily in appellate judgments on the exceptions to date, I argue that such a purposive approach to interpretation of the exceptions could assist the judiciary to reach fair, balanced and consistent decisions in this highly contested area of the law by inviting consideration of the relationships among an employer’s ethos, its employees and the religious group it serves, and by encouraging engagement with the discriminatory impacts which an exercise of these rights may entail.
Post-Doc Interviews in the Life Sciences: An Often-Overlooked Process that Is Susceptible to Bias
Authors: Burnett N P , Combes S A
Published: December 2019

Synopsis

Post-doctoral training is a critical career stage for researchers in the life sciences, yet interviewing for a post-doctoral position is largely an unregulated process. Without regulation, interviews are susceptible to unconscious biases that may lead to discrimination against certain demographic groups (e.g., women and minorities). Using data from an online survey of post-docs, we show that interview procedures for post-doctoral positions in the life sciences are correlated with several factors (e.g., candidate demographics) in ways that may bias the outcome of interviews. We discuss key components of interviews and suggest that conducting standardized, well-planned interviews that are less susceptible to unconscious biases may help increase the retention of women and under-represented minorities in the life sciences.
Systemist International Relations
Authors: James Patrick
Published: December 2019

Systemist international relations (SIR) is put forward as a potential solution to short- and long-term problems faced by the discipline of international relations (IR). SIR responds to the immediate difficulties that stem from an impasse between advocates of analytic eclecticism and skeptics who prefer paradigmatic research. The more sustained challenges posed by the size and complexity of IR also can be met through implementation of SIR, which entails a graphic turn. Along those lines, the Visual International Relations Project (VIRP) is creating an archive of one-page graphic summaries for cause and effect as conveyed in respective publications. The VIRP aims toward an improved state of communication in the field based on such visual representations.
Phase-feeding strategies based on lysine specifications for grow-finish pigs1
Authors: Menegat Mariana B , Dritz Steve S , Tokach Mike D , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D
Published: December 2019

Four experiments were conducted using 1,100 to 1,188 pigs each (PIC 359 × 1,050) from ~27 to 127 kg BW to evaluate phase-feeding strategies based on Lys specifications and number of dietary phases for grow-finish pigs. Different phase-feeding strategies were used in each experiment with treatments consisting of a combination of 3 Lys specifications at 96%, 98%, or 100% of estimated requirement for growth rate and 4 phase-feeding strategies with 1, 2, 3, or 4 dietary phases. A single-phase-feeding strategy reduced (P < 0.05) overall growth performance, live BW, and HCW whether Lys specifications were at 98% or 100% of estimated requirements compared with multi-phase-feeding strategies. Lysine specifications at 96% of estimated requirements in a 4-phase-feeding strategy reduced (P < 0.05) overall growth performance compared with feeding strategies with Lys at 100% of estimated requirements, unless Lys specifications were increased to 100% of estimated requirements in the late finishing phase. Lysine specifications at 98% or 100% of estimated requirements in a 2-, 3- or 4-phase feeding strategy led to similar (P < 0.05) overall growth rate, live BW, and HCW of grow-finish pigs. Pigs fed 1, 2, or 3-phase feeding strategies or feeding strategies with Lys below the requirements in early grow finish had improved growth performance driven by improved feed efficiency in the period following low Lys levels, indicating the occurrence of compensatory growth. For carcass characteristics, there was no evidence (P > 0.10) for differences in carcass yield, back fat, loin depth, or lean percentage across feeding strategies in any of the experiments. In conclusion, phase-feeding strategies provide performance advantages over feeding a single dietary phase throughout the grow-finish period. Simplification of feeding strategies from 4 to 3 or 2 dietary phases with Lys specifications at 98% to 100% of estimated requirements for growth rate does not compromise overall growth performance and carcass characteristics of grow-finish pigs from 27 to 127 kg BW. Although, using feeding programs with fewer dietary phases and Lys set slightly below the requirements can compromise growth performance if initial BW and feed intake in the grow-finish period are lower than expected.
Vectorial Transmission of Malaria in Major Districts of Côte d’Ivoire
Authors: Assouho Konan F , Adja Akré M , Guindo-Coulibaly Négnorogo , Tia Emmanuel , Kouadio Affoué M N , Zoh Dounin D , Koné Moussa , Kessé Nestor , Koffi Bernard , Sagna André B , Poinsignon Anne , Yapi Ahoua
Published: December 2019

To better understand the influence of periodic mass distribution of Long-Lasting Insecticidal Nets (LLINs) on malaria transmission, a 1-yr entomological survey was conducted in three major districts of Côte d’Ivoire. Mosquitoes were sampled by Human Landing Catches (HLC) in urban and rural areas of San Pedro and Abidjan (coastal), and in Yamoussoukro (central). Mosquitoes were identified morphologically and by molecular methods. The Plasmodium falciparum circumsporozoite (CSP) indices were measured by ELISA, and the Entomological Inoculation Rates (EIR) were calculated for each species and area. Anopheles gambiae s.l. Giles (Diptera: Culicidae) and Anopheles nili Theobald (Diptera: Culicidae) were identified in coastal districts, while An. gambiae s.l. and Anopheles funestus Giles (Diptera: Culicidae) were reported in the central district. In urban areas, malaria vectors showed a low aggressiveness (<10 bites per person per night), except in Yamoussoukro, where up to 18.9 b/p/n were recorded. The annual EIR was higher in the central urban area (138.7 infected bites per person per year) than in coastal ones (10–62 ib/p/n). In rural areas, malaria vectors were highly aggressive with an average 13 b/p/n for An. gambiae s.l, 21.2 b/p/n for An. nili and 12 b/p/n for An. funestus. The annual EIR ranged between 94.9 and 193.4 infected bites per person per year. This work indicates that, despite repeated mass distribution of LLINs, malaria transmission remains high and heterogeneous across Côte d’Ivoire. Malaria transmission was lower in coastal urban areas than in the central one, and remains high rural areas where two additional Anopheles vectors are involved in malaria transmission.
Biodiversity of Medically Important Calyptratae Flies (Diptera: Schizophora) in Hospitals in the Northern Coastline of the Persian Gulf, Iran
Authors: Sanei-Dehkordi Alireza , Soleimani-Ahmadi Moussa , Cheshmposhan Abdolrahim , Akbarzadeh Kamran
Published: December 2019

Domestic filth flies play a significant role in the transmission of nosocomial infections and cause nosocomial myiasis in hospitals worldwide. This descriptive cross-sectional study was conducted in five hospitals of the Hormozgan province, of southern Iran. Adult flies were collected by bottle fly traps. Specimens were pinned and morphologically identified using the relevant keys. Species biodiversity indices including Menhinick (M), Shannon–Wiener (H’), Pielou’s Evenness (E), and Simpson’s diversity (D) were calculated. Totally 2,298 individuals of 18 species of flies belonging to nine genera and four families were collected. Chrysomya megacephala was the most frequent species (37.3%), followed by Chrysoma albiceps (24.8%), Fannia canicularis (5.1%), Musca domestica (4.8%), Sarcophaga aegyptica (3.7%), and 15 other species (24.4%). Our results show a high diversity and abundance of domestic filth flies in the studied hospitals. Relatively similar data derived from various hospitals in this study can show the uniformity in sanitary conditions of hospitals. On the other hand, estimation of diversity indices alarmed for potential of dominant species in fly communities in hospital environments.
Spatiotemporal variation in leaf size and shape in response to climate
Authors: Li Yaoqi , Zou Dongting , Shrestha Nawal , Xu Xiaoting , Wang Qinggang , Jia Wen , Wang Zhiheng
Published: December 2019

Aims

Morphological variation of leaves is a key indicator of plant response to climatic change. Leaf size and shape are associated with carbon, water and energy exchange of plants with their environment. However, whether and how leaf size and shape responded to climate change over the past decades remains poorly studied. Moreover, many studies have only explored inter- but not intraspecific variation in leaf size and shape across space and time.

Methods

We collected >6000 herbarium specimens spanning 98 years (1910–2008) in China for seven representative dicot species and measured their leaf length and width. We explored geographical patterns and temporal trends in leaf size (i.e. leaf length, leaf width and length × width product) and shape (i.e. length/width ratio), and investigated the effects of changes in precipitation and temperature over time and space on the variation in leaf size and shape.

Important Findings

After accounting for the effects of sampling time, leaf size decreased with latitude for all species combined, but the relationship varied among species. Leaf size and shape were positively correlated with temperature and precipitation across space. After accounting for the effects of sampling locations, leaf size of all species combined increased with time. Leaf size changes over time were mostly positively correlated with precipitation, whereas leaf shape changes were mostly correlated with temperature. Overall, our results indicate significant spatial and temporal intraspecific variation in leaf size and shape in response to climate. Our study also demonstrates that herbarium specimens collected over a considerable period of time provide a good resource to study the impacts of climate change on plant morphological traits.
ABA perception is modulated by membrane receptor-like kinases
Authors: Konopka-Postupolska Dorota , Dobrowolska Grazyna
Published: December 2019

This article comments on:

Shang Y, Yang D, Ha Y, Shin H-Y, Nam KH. 2020. RPK1 and BAK1 sequentially form complexes with OST1 to regulate ABA-induced stomatal closure. Journal of Experimental Botany 71, 1491–1502.
Toward Personalized Medicine Implementation: Survey of Military Medicine Providers in the Area of Pharmacogenomics
Authors: DeLuca Jesse , Selig Daniel , Poon Lucas , Livezey Jeffrey , Oliver Thomas , Barrett John , Turner Clesson , Hellwig Lydia
Published: December 2019

Introduction

Personalized medicine is the right treatment, to the right patient, at the right dose. Knowledge of genetic predisposition to variable metabolism and distribution of drugs within the body is currently available as pharmacogenomic testing and is one of the pillars of personalized medicine. Pharmacogenomic testing is growing. It has become part of guidelines for dosing on FDA labels and has been used by health care organizations to improve outcomes and reduce adverse events. Additionally, it has been FDA approved for direct-to-consumer purchase and has been cause of concern of patient self-dosing and medication changes. Presumably in the near future, pharmacogenomics will be impressed upon the military health system (MHS) provider from either a top-down, command requested, or from a bottom-up, patient requested, approach. To date, widespread implementation of pharmacogenomic testing does not seem to be established within the MHS. This survey sheds light on the knowledge, exposure, use, comfort, and interest among family medicine providers in the MHS. It compares similar results in other national and international surveys and compares results among a small subset of residents to staff.

Materials and Methods

The questions were part of a larger survey conducted by the Clinical Investigations Committee of the Uniformed Services Academy of Family Physicians (USAFP) at the USAFP 2019 annual meeting. The study received approval from the Uniformed Services University Institutional Review Board. Submitted questions were written using multiple choice, fill-in, five-point Likert scale, and best answer. Direct results are reported as well as chi-square statistics for categorical data with statistical significance to attain a P-value of < 0.05.

Results

Among the 532 USAFP-registered conference attendees eligible to complete the survey, 387 attendees responded to the survey, for a response rate of 72.7%. Some results included were a knowledge question in which 37% of respondents answered correctly. Less than half of respondents agreed that they could define pharmacogenomics, and resident respondents were more likely to have received teaching in graduate medical education. Additionally, 12% of providers responded to being exposed to direct-to-consumer results, and 28% of those exposed were influenced to change medications, while 14% were influenced to change medications on multiple occasions. Chi-square comparisons resulted in statistically significant direct relationships to exposure to direct to consumer testing, previous training, and confidence of those that answered the knowledge question correctly.

Conclusions

This survey establishes a baseline for the possible needs associated with implementation of a pharmacogenomic program, and it argues an actionable level for the use of pharmacogenomics among the patient population within the MHS.
How cold gas continuously entrains mass and momentum from a hot wind
Authors: Gronke Max , Oh S Peng
Published: December 2019

The existence of fast moving, cold gas ubiquitously observed in galactic winds is theoretically puzzling, since the destruction time of cold gas is much smaller than its acceleration time. In previous work, we showed that cold gas can accelerate to wind speeds and grow in mass if the radiative cooling time of mixed gas is shorter than the cloud destruction time. Here, we study this process in much more detail, and find remarkably robust cloud acceleration and growth in a wide variety of scenarios. Radiative cooling, rather than the Kelvin–Helmholtz instability, enables self-sustaining entrainment of hot gas on to the cloud via cooling-induced pressure gradients. Indeed, growth peaks when the cloud is almost co-moving. The entrainment velocity is of order the cold gas sound speed, and growth is accompanied by cloud pulsations. Growth is also robust to the background wind and initial cloud geometry. In an adiabatic Chevalier-Clegg type wind, for instance, the mass growth rate is constant. Although growth rates are similar with magnetic fields, cloud morphology changes dramatically, with low density, magnetically supported filaments, which have a small mass fraction but dominate by volume. This could bias absorption line observations. Cloud growth from entraining and cooling hot gas can potentially account for the cold gas content of the circumgalactic medium (CGM). It can also fuel star formation in the disc as cold gas recycled in a galactic fountain accretes and cools halo gas. We speculate that galaxy-scale simulations should converge in cold gas mass once cloud column densities of N ∼ 1018 cm−2 are resolved.
O(3P) + CO2 scattering cross-sections at superthermal collision energies for planetary aeronomy
Authors: Gacesa Marko , Lillis R J , Zahnle K J
Published: December 2019

We report new elastic and inelastic cross-sections for O(3P) + CO2 scattering at collision energies from 0.03 to 5 eV, of major importance to O escape from Mars, Venus, and CO2-rich atmospheres. The cross-sections were calculated from first principles using three newly constructed ab initio potential energy surfaces correlating to the lowest energy asymptote of the complex. The surfaces were restricted to a planar geometry with the CO2 molecule assumed to be in linear configuration fixed at equilibrium. Quantum-mechanical coupled-channel formalism with a large basis set was used to compute state-to-state integral and differential cross-sections for elastic and inelastic O(3P) + CO2 scattering between all pairs of rotational states of CO2 molecule. The elastic cross-sections are 35 per cent lower at 0.5 eV and more than 50 per cent lower at 4 + eV than values commonly used in studies of processes in upper and middle planetary atmospheres of Mars, Earth, Venus, and CO2-rich planets. Momentum transfer cross-sections, of interest for energy transport, were found to be proportionally lower than predicted by mass scaling.
Improved calculations of electron–ion bremsstrahlung Gaunt factors for astrophysical applications
Authors: Chluba Jens , Ravenni Andrea , Bolliet Boris
Published: December 2019

Electron–ion bremsstrahlung (free–free) emission and absorption occur in many astrophysical plasmas for a wide range of physical conditions. This classical problem has been studied multiple times, and many analytical and numerical approximations exist. However, accurate calculations of the transition from the non-relativistic to the relativistic regime remain sparse. Here we provide a comprehensive study of the free–free Gaunt factors for ions with a low charge (Z ≤ 10). We compute the Gaunt factor using the expressions for the differential cross-section given by Elwert and Haug (EH) and compare to various limiting cases. We develop a new software package, BRpack, for direct numerical applications. This package uses a combination of pre-computed tables and analytical approximations to efficiently cover a wide range of electron and photon energies, providing a representation of the EH Gaunt factor to better than $0.03{{\ \rm per\ cent}}$ precision for Z ≤ 2. Our results are compared to those of previous studies highlighting the improvements achieved here. BRpack should be useful in computations of spectral distortions of the cosmic microwave background, radiative transfer problems during reionization or inside galaxy clusters, and the modelling of galactic free–free foregrounds. The developed computational methods can furthermore be extended to higher energies and ion charge.
Anti–PD-L1 antibody direct activation of macrophages contributes to a radiation-induced abscopal response in glioblastoma
Authors: Ene Chibawanye I , Kreuser Shannon A , Jung Miyeon , Zhang Huajia , Arora Sonali , White Moyes Kara , Szulzewsky Frank , Barber Jason , Cimino Patrick J , Wirsching Hans-Georg , Patel Anoop , Kong Paul , Woodiwiss Timothy R , Durfy Sharon J , Houghton A McGarry , Pierce Robert H , Parney Ian F , Crane Courtney A , Holland Eric C
Published: December 2019

Background

Most glioblastomas recur near prior radiation treatment sites. Future clinical success will require achieving and optimizing an “abscopal effect,” whereby unirradiated neoplastic cells outside treatment sites are recognized and attacked by the immune system. Radiation combined with anti–programmed cell death ligand 1 (PD-L1) demonstrated modest efficacy in phase II human glioblastoma clinical trials, but the mechanism and relevance of the abscopal effect during this response remain unknown.

Methods

We modified an immune-competent, genetically driven mouse glioma model (forced platelet derived growth factor [PDGF] expression + phosphatase and tensin homolog loss) where a portion of the tumor burden is irradiated (PDGF) and another unirradiated luciferase-expressing tumor (PDGF + luciferase) is used as a readout of the abscopal effect following systemic anti–PD-L1 immunotherapy. We assessed relevance of tumor neoepitope during the abscopal response by inducing expression of epidermal growth factor receptor variant III (EGFRvIII) (PDGF + EGFRvIII). Statistical tests were two-sided.

Results

Following radiation of one lesion, anti–PD-L1 immunotherapy enhanced the abscopal response to the unirradiated lesion. In PDGF-driven gliomas without tumor neoepitope (PDGF + luciferase, n = 8), the abscopal response occurred via anti–PD-L1 driven, extracellular signal-regulated kinase–mediated, bone marrow–derived macrophage phagocytosis of adjacent unirradiated tumor cells, with modest survival implications (median survival 41 days vs radiation alone 37.5 days, P = 0.03). In PDGF-driven gliomas with tumor neoepitope (PDGF + EGFRvIII, n = 8), anti–PD-L1 enhanced abscopal response was associated with macrophage and T-cell infiltration and increased survival benefit (median survival 36 days vs radiation alone 28 days, P = 0.001).

Conclusion

Our results indicate that anti–PD-L1 immunotherapy enhances a radiation- induced abscopal response via canonical T-cell activation and direct macrophage activation in glioblastoma.
Radiosensitizers in the temozolomide era for newly diagnosed glioblastoma
Authors: Mathen Peter , Rowe Lindsay , Mackey Megan , Smart DeeDee , Tofilon Philip , Camphausen Kevin
Published: December 2019

Glioblastoma (GBM) is a challenging diagnosis with almost universally poor prognosis. Though the survival advantage of postoperative radiation (RT) is well established, around 90% of patients will fail in the RT field. The high likelihood of local failure suggests the efficacy of RT needs to be improved to improve clinical outcomes. Radiosensitizers are an established method of enhancing RT cell killing through the addition of a pharmaceutical agent. Though the majority of trials using radiosensitizers have historically been unsuccessful, there continues to be interest with a variety of approaches having been employed. Epidermal growth factor receptor inhibitors, histone deacetylase inhibitors, antiangiogenic agents, and a number of other molecularly targeted agents have all been investigated as potential methods of radiosensitization in the temozolomide era. Outcomes have varied both in terms of toxicity and survival, but some agents such as valproic acid and bortezomib have demonstrated promising results. However, reporting of results in phase 2 trials in newly diagnosed GBM have been inconsistent, with no standard in reporting progression-free survival and toxicity. There is a pressing need for investigation of new agents; however, nearly all phase 3 trials of GBM patients of the past 25 years have demonstrated no improvement in outcomes. One proposed explanation for this is the selection of agents lacking sufficient preclinical data and/or based on poorly designed phase 2 trials. Radiosensitization may represent a viable strategy for improving GBM outcomes in newly diagnosed patients, and further investigation using agents with promising phase 2 data is warranted.
The painful truth: work-related musculoskeletal disorders in Australian surgeons
Authors: Grant K M K , Vo T , Tiong L U
Published: December 2019

Background

Work-related musculoskeletal disorder (WRMD) is a collective term for preventable work-related disorders that cause musculoskeletal symptoms. The growing prevalence of these disorders amongst surgeons has been labelled an ‘impending epidemic’ and currently there is a lack of awareness and no existing intervention programme.

Aims

To estimate prevalence and identify risk factors for developing work related neck, shoulder and upper back pain in surgeons.

Methods

Cross-sectional questionnaire survey of surgeons and surgical trainees across Australia. We collected data on demographics, nature of regular operative work, hours working/operating per week, and prevalence and severity of musculoskeletal disorders based on the Nordic Musculoskeletal Questionnaire [1]. We assessed associations between categorical predictors and pain over 7 days and 12 months using Pearson’s chi-square test. We assessed associations involving continuous variables using Student’s t-test. Tests were two-tailed and assessed at the 5% alpha level.

Results

From ~2058 recipients, 329 (16%) responded; 137 (42%) surgeons reported shoulder, neck or upper back pain in a 7-day period, 245 (75%) reported pain and 101 (31%) had pain preventing normal work in a 12-month period. Significant predictors of increased prevalence included female sex (P < 0.001), decreasing age (P < 0.01) and fewer years working (P < 0.01). Body mass index (BMI) and mode of practise did not significantly affect prevalence of WRMDs.

Conclusions

The prevalence of WRMDs amongst surgeons is high, females and younger surgeons reporting increased rates. There is a need to build awareness and educate surgical trainees about WRMDs.
Systematic Review of the Effectiveness of Lumbar Medial Branch Thermal Radiofrequency Neurotomy, Stratified for Diagnostic Methods and Procedural Technique
Authors: Schneider Byron J , Doan Lisa , Maes Marc K , Martinez Kevin R , Gonzalez Cota Alan , Bogduk Nikolai ,   on behalf of the Standards Division of the Spine Intervention Society
Published: December 2019

Objective

To determine the effectiveness of lumbar medial branch thermal radiofrequency neurotomy based on different selection criteria and procedural techniques.

Design

Comprehensive systematic review.

Methods

A comprehensive literature search was conducted, and all authors screened and evaluated the studies. The Grades of Recommendation, Assessment, Development, and Evaluation system was used to assess all eligible studies.

Outcome Measures

The primary outcome measure assessed was the success rate of the procedure, defined by varying degrees of pain relief following neurotomy. Data are stratified by number of diagnostic blocks and degree of pain relief, as well as procedural technique with perpendicular or parallel placement of electrodes.

Results

Results varied by selection criteria and procedural technique. At six months, 26% of patients selected via single medial branch block with 50% pain relief and treated via perpendicular technique achieved at least 50% pain relief; 49% of patients selected via dual medial branch blocks with 50% pain relief and treated via parallel technique achieved at least 50% pain relief. The most rigorous patient selection and technique—two diagnostic medial branch blocks with 100% pain relief and parallel electrode placement—resulted in 56% of patients experiencing 100% relief of pain at six months.

Conclusions

This comprehensive systematic review found differences in the effectiveness of lumbar medial branch radiofrequency neurotomy when studies were stratified by patient selection criteria and procedural technique. The best outcomes are achieved when patients are selected based on high degrees of pain relief from dual medial branch blocks with a technique employing parallel electrode placement.
The Debt Burden of Entry-Level Physical Therapists
Authors: Ambler Steven B
Published: December 2019

Background

The growing student debt of physical therapists entering the workforce, coupled with the growth in projected need, raises concerns about where and how entry-level physical therapists will practice and if these choices will be affected by their debt burden.

Objective

The purpose of this study was to identify the debt profile of entry-level physical therapists and explore relationships between student debt and clinical practice setting choices.

Methods

This study utilized a cross-sectional survey design to identify debt profiles and explore relationships between student debt and the clinical practice choices of entry-level physical therapists.

Results

The mean debt-to-income ratio based on the total reported educational debt was 197% (93%). The most frequently reported debt range for doctor of physical therapy (DPT) debt and total educational debt was $100,000 to $124,999. Despite the setting itself being rated as the most important factor (83%), 28% of participants reported debt as a barrier to their desired practice setting. In addition, when considering job choice overall, 57% of the participants reported that their student debt has had an effect on their decision.

Limitations

This study is limited by its small sample size, originating from 1 state, and being taken by convenience from a special interest group. Data were collected via an anonymous survey, which increases the risk of selection bias. In addition, there are further personal, family, and institutional characteristics that were not collected in this study, which may influence the interaction between student debt and clinical practice choices.

Conclusion

The results of this study suggest that practice setting choice may be affected by physical therapist student debt, and student debt may be a barrier overall to practice and career choices in physical therapy.
Determinants of Public Attitudes Towards Immigrants: Evidence from Arab Barometer
Authors: Ceyhun Hüseyin Emre
Published: December 2019

What factors determine public opinion towards immigrants? This inquiry is especially crucial in the context of developing countries since they hold 80 per cent of global refugee populations. Lebanon, with the burden on its shoulders due to hosting about one million Syrians, offers a unique case to study the mechanisms driving the formation of attitudes towards immigrants. In this article, I examine how Syrian density is associated with Lebanese attitudes towards immigrants. Using Arab Barometer Wave IV data (2016), I test three arguments linking public attitudes to natives’ economic, security, and sectarian concerns. My analysis suggests that there is no relationship between employment status and negative attitudes towards immigrants. Instead, I argue that perceived economic situation and sense of security provide better mechanisms for the formation of natives’ attitudes towards immigrants. Moreover, I present the observational evidence that Lebanese attitudes towards immigrants are driven by one’s sectarian affiliation. Notably, Christians are more likely to adopt positive attitudes towards immigrants as Syrian density increases, compared with Shi’as more likely to cite prejudice.
Application of a sequence-based taxonomic classification method to uncultured and unclassified marine single-stranded RNA viruses in the order Picornavirales
Authors: Vlok Marli , Lang Andrew S , Suttle Curtis A
Published: December 2019

Metagenomics has altered our understanding of microbial diversity and ecology. This includes its applications to viruses in marine environments that have demonstrated their enormous diversity. Within these are RNA viruses, many of which share genetic features with members of the order Picornavirales; yet, very few of these have been taxonomically classified. The only recognized family of marine RNA viruses is the Marnaviridae, which was founded based on discovery and characterization of the species Heterosigma akashiwo RNA virus. Two additional genera of marine RNA viruses, Labyrnavirus (one species) and Bacillarnavirus (three species), were subsequently defined within the order Picornavirales but not assigned to a family. We have defined a sequence-based framework for taxonomic classification of twenty marine RNA viruses into the family Marnaviridae. Using RNA-dependent RNA polymerase (RdRp) phylogeny and distance-based analyses, we assigned the genera Labyrnavirus and Bacillarnavirus to the family Marnaviridae and created four additional genera in the family: Locarnavirus (four species), Kusarnavirus (one species), Salisharnavirus (four species) and Sogarnavirus (six species). We used pairwise capsid protein comparisons to delineate species within families, with 75 per cent identity as the species demarcation threshold. The family displays high sequence diversities and Jukes–Cantor distances for both the RdRp and capsid genes, suggesting that the classified viruses are not representative of all of the virus diversity within the family and that there are many more extant taxa. Our proposed taxonomic framework provides a sound classification system for this group of viruses that will have broadly applicable principles for other viral groups. It is based on sequence data alone and provides a robust taxonomic framework to include viruses discovered via metagenomic studies, thereby greatly expanding the realm of viruses subject to taxonomic classification.
Black Rot of Sweetpotato: A Comprehensive Diagnostic Guide
Authors: Stahr Madison N. , Quesada-Ocampo Lina M.
Published: December 2019

Black rot of sweetpotato (Ipomoea batatas) has been considered one of the most historically devastating diseases of the crop. The pathogen, Ceratocystis fimbriata (Ellis and Halst), is able to infect a variety of hosts including morning glory (Ipomoea sp.), coffee (Coffea sp.), and mango (Mangifera indica) over a wide geographic range. The slow-growing nature of the pathogen can lead to difficulty in isolating and maintaining cultures of the fungus. Thus, the objective for this diagnostic guide is to provide information about effective techniques for pathogen isolation, identification, storage, and pathogenicity testing as well as describe the host and geographic range, taxonomy, and disease in sweetpotato.
Defective sarcomere organization and reduced larval locomotion and fish survival in slow muscle heavy chain 1 (smyhc1) mutants
Authors: Li Siping , Wen Haishen , Du Shaojun
Published: December 2019

Zebrafish skeletal muscles are broadly divided into slow‐twitch and fast‐twitch muscle fibers. The slow fibers, which express a slow fiber‐specific myosin heavy chain 1 (Smyhc1), are the first group of muscle fibers formed during myogenesis. To uncover Smyhc1 function in muscle growth, we generated three mutant alleles with reading frame shift mutations in the zebrafish smyhc1 gene using CRISPR. The mutants showed shortened sarcomeres with no thick filaments and M‐lines in slow fibers of the mutant embryos. However, the formation of slow muscle precursors and expression of other slow muscle genes were not affected and fast muscles appeared normal. The smyhc1 mutant embryos and larvae showed reduced locomotion and food intake. The mutant larvae exhibited increased lethality of incomplete penetrance. Approximately 2/5 of the homozygous mutants were viable and grew into reproductive adults. These adult mutants displayed a typical pattern of slow and fast muscle fiber distribution, and regained normal slow muscle formation. Together, our studies indicate that Smyhc1 is essential for myogenesis in embryonic slow muscles, and loss of Smyhc1 results in defective sarcomere assembly, reduces larval motility and fish survival, but has no visible impact on muscle growth in juvenile and adult zebrafish that escape the larval lethality.