bluuebunny/crossref_metadata_2025 huggingface dataset:
Raman suppression in high-power fiber oscillators by femtosecond-written chirped and tilted fiber Bragg gratings
Authors: Li Hao , Chen Jinbao , Ye Xinyu , Rao Binyu , Wang Meng , Wu Baiyi , Gao Chenhui , Chen Zilun , Wang Zefeng
Published: November 2023

Realizing stimulated Raman scattering (SRS) suppression is a key topic for high-power fiber lasers. Here, we report an effective and simple strategy for SRS suppression using chirped and tilted fiber Bragg gratings (CTFBGs) in high-power fiber oscillators while maintaining the compactness and stability of the system. The CTFBG is inserted on the side of a cavity mirror FBG without cutting the gain fiber. To improve power handling capability, the CTFBG and cavity mirror FBGs are inscribed by femtosecond (fs) lasers. The optimal SRS suppression effect can be realized when the CTFBG is inserted into the resonant cavity and on the side of the output coupler FBG. The SRS threshold is increased by approximately 11% with an SRS suppression ratio of nearly 14 dB. Moreover, the output power of the fiber oscillator is improved to 3.5 kW, which is the maximum power achieved in fiber oscillators with SRS suppression using CTFBGs, to the best of our knowledge. The temperature of the air-cooled CTFBG is 50.2 °C, which has the potential to handle higher power. This work provides new insights for suppressing SRS in fiber oscillators, promoting the application of CTFBGs in high-power lasers.
Self-healing FBG sensor network fault-detection based on a multi-class SVM algorithm
Authors: Hu Jinhua , Wang Boying , Di Kangjian , Zou Jun , Ren Danping , Zhao Jijun
Published: November 2023

We propose a three-layer ring architecture with enhanced reconfigurable capabilities for fiber Bragg grating (FBG) sensor networks. The proposed network is capable of self-healing when three fiber links fail. In addition to self-healing, soft faults in the FBG sensors can be detected using a multi-classification support vector machine (multi-class SVM) algorithm. The detection accuracy reached 99%. Additionally, we used an artificial neural network (ANN) reliability estimation model to estimate the reliability of the FBG self-healing network. The results show that the ANN reliability analysis model can accurately estimate the reliability of the architecture at a reasonable cost.
Optical wavefront phase-tilt measurement using Si-photonic waveguide grating couplers
Authors: Janz S. , Xu D. X. , Grinberg Y. , Wang S. , Vachon M. , Cheben P. , Schmid J. H. , Melati D.
Published: November 2023

Silicon photonic wavefront phase-tilt sensors for wavefront monitoring using surface coupling grating arrays are demonstrated. The first design employs the intrinsic angle dependence of the grating coupling efficiency to determine local wavefront tilt, with a measured sensitivity of 7 dB/°. The second design connects four gratings in an interferometric waveguide circuit to determine incident wavefront phase variation across the sensor area. In this device, one fringe spacing corresponds to 2° wavefront tilt change. These sensor elements sample a wavefront incident on the chip surface without the use of bulk optic elements, fiber arrays, or imaging arrays. Both sensor elements are less than 60 µm across and are potential unit cell sensor elements for large arrays that monitor wavefront shape across an image or pupil plane in adaptive optics systems for free-space optical communications, astronomy, and beam pointing applications.
Multipartite entanglement encoded in the photon-number basis by sequential excitation of a three-level system
Authors: Santos Alan C. , Schneider Christian , Bachelard R. , Predojević Ana , Antón-Solanas Carlos
Published: November 2023

We propose a general scheme to generate entanglement encoded in the photon-number basis, via a sequential resonant two-photon excitation of a three-level system. We apply it to the specific case of a quantum dot three-level system, which can emit a photon pair through a biexciton–exciton cascade. The state generated in our scheme constitutes a tool for secure communication, as the multipartite correlations present in the produced state may provide an enhanced rate of secret communication with respect to a perfect GHZ state.
Periodic poling of thin-film lithium tantalate by applying a high-voltage electric field
Authors: Chen Haiwei , Zhao Mengwei , Xie Haotian , Su Yawen , Ye Zhilin , Ma Jianan , Nie Yuefeng , Niu Yunfei , Zhan Peng , Zhu Shining , Hu Xiaopeng
Published: November 2023

Periodically poled lithium tantalate on insulator (PPLTOI) was
successfully fabricated by applying a high-voltage electric field. The
shape of the electrode, which determines the electric field
distribution, as well as the poling time, and the strength of the
electric field, are investigated in detail for the fabrication of
periodically poled LTOI. By optimizing the poling parameters, the duty
cycle of the inverted domain can be flexibly adjusted as well as be
controlled to the optimal value of 50%. Moreover, the
fabricated domain structure is uniform, and the standard deviation is
less than 4.8%. The study presented in this work will pave the
way for applications of LTOI in nonlinear integrated photonics.
Color characterization of infrared two-photon vision
Authors: Gil Pedro , Tabernero Juan , Manzanera Silvestre , Schwarz Christina , Artal Pablo
Published: November 2023

Humans have the ability to perceive pulsed near-infrared (NIR) light as visible light with about half the wavelength through a process known as two-photon (2P) absorption. Although it has been known for several decades, color perception in 2P vision remains uncharacterized. In this study, we conducted color matching experiments between pulsed NIR light and continuous visible light. We investigated seven NIR wavelengths ranging from 880 to 1100 nm, along with three radiant power values at the pupil plane, varying from 10 to 30 µW. Through these experiments, we obtained chromatic coordinates, chromaticity diagrams, dominant wavelengths, and average spectra. We found a pronounced correlation between perceived hue and wavelength, with hues shifting from reddish purple at 880 nm to blue, green, and yellowish green at 1100 nm. Moreover, we observed a relationship between hue and power for the wavelengths closer to the visible end of the spectrum. This phenomenon appears to be a consequence of the intensity-dependent ratio between the single photon (1P) and 2P absorption efficiencies of the visual pigments.
High-responsivity on-chip waveguide coupled germanium photodetector for 2  μm waveband
Authors: Wang Jianing , Wang Xi , Li Yihang , Yang Yanfu , Song Qinghai , Xu Ke
Published: November 2023

Recently, the emerging 2 μm waveband has gained increasing interest due to its great potential for a wide scope of applications. Compared with the existing optical communication windows at shorter wavelengths, it also offers distinct advantages of lower nonlinear absorption, better fabrication tolerance, and larger free carrier plasma effects for silicon photonics, which has been a proven device technology. While much progress has been witnessed for silicon photonics at the 2 μm waveband, the primary challenge still exists for on-chip detectors. Despite the maturity and compatibility of the waveguide coupled photodetectors made of germanium, the 2 μm regime is far beyond its cutoff wavelength. In this work, we demonstrate an efficient and high-speed on-chip waveguide-coupled germanium photodetector operating at the 2 μm waveband. The weak sub-bandgap absorption of epitaxial germanium is greatly enhanced by a lateral separation absorption charge multiplication structure. The detector is fabricated by the standard process offered by a commercial foundry. The device has a benchmark performance with responsivity of 1.05 A/W and 3 dB bandwidth of 7.12 GHz, which is able to receive high-speed signals with up to 20 Gbit/s data rate. The availability of such an efficient and fast on-chip detector circumvents the barriers between silicon photonic integrated circuits and the potential applications at the 2 μm waveband.
Comparison of door-to-door and fixed-point delivery of azithromycin distribution for child survival in Niger: A cluster-randomized trial
Authors: Arzika Ahmed M. , Maliki Ramatou , Amza Abdou , Karamba Alio , Gallo Nasser , Aichatou Bawa , Sara Ismael Issa , Beidi Diallo , Haroun Laminou Maliki , Oumarou Farissatou , Lebas Elodie , Peterson Brittany , Colby Emily , Nguyen William , Liu Zijun , Fitzpatrick Meagan C. , Arnold Benjamin F. , Lietman Thomas M. , O’Brien Kieran S. ,   the AVENIR Study Group
Published: November 2023

Recent evidence indicates mass azithromycin distribution reduces under-5 mortality. This intervention is being considered for child survival programs in high mortality sub-Saharan African settings. The delivery approach used in prior studies required a full-time census and distribution team, which is not feasible for most programs. To determine the optimal programmatic approach to delivery, this study aimed to compare treatment coverage, costs, and acceptability of different delivery approaches with existing community health workers (CHWs). This cluster-randomized trial included rural and peri-urban communities in Dosso, Niger (clinicaltrials.gov, NCT04774991). A random sample of 80 eligible communities was randomized 1:1 to biannual door-to-door or fixed-point delivery of oral azithromycin to children 1–59 months old over 1 year. Data analysts alone were masked given the nature of the intervention. The primary outcome was community-level treatment coverage defined as the number of children treated recorded by CHWs divided by the number of eligible children determined using a post-distribution census. Costs were monitored through routine administrative data collection and micro-costing. The census included survey questions on intervention acceptability among caregivers, community leaders, and CHWs. After randomization, 1 community was excluded due to inaccuracies in available administrative data, resulting in 39 communities receiving door-to-door delivery. At the second distribution, community-level mean treatment coverage was 105% (SD 44%) in the door-to-door arm and 92% (SD 20%) in the fixed-point arm (Mean difference 13%, 95% CI -2% to 28%, P-value = 0.08). The total cost per dose delivered was $1.91 in the door-to-door arm and $2.51 in the fixed-point arm. Indicators of acceptability were similar across stakeholder groups in both arms, with most respondents in each group indicating a preference for door-to-door. Overall, door-to-door delivery is the preferred approach to azithromycin distribution in this setting and might reach more children at a lower cost per dose delivered than fixed-point.

Trial Registration: clinicaltrials.gov NCT04774991.
Remote evaluation of STH program coverage: Experiences from the DeWorm3 study, India
Authors: Aruldas Kumudha , Ramesh Rohan Michael , Oswald William E. , Janagaraj Venkateshprabhu , Titus Angelin , Johnson Jabaselvi , Saxena Malvika , Israel Gideon John , Halliday Katherine , Walson Judd L. , Means Arianna Rubin , Ajjampur Sitara Swarna Rao
Published: November 2023

Background

The DeWorm3 trial is a multi-country study testing the feasibility of interrupting transmission of soil-transmitted helminths by community-wide mass drug administration (cMDA). Treatment coverage during cMDA delivery was validated by in-person coverage evaluation surveys (CES) after each round of treatment. A mobile phone-based CES was carried out in India when access to households was restricted during the COVID-19 lockdown.

Methods

Two focus group discussions were conducted with the survey implementers to document their experiences of conducting phone-based CES via mobile-phone voice calls.

Principal findings

In the phone-based CES, only 56% of sampled households were reached compared to 89% during the in-person CES (89%). This was due to phone numbers being wrongly recorded, or calls being unanswered leading to a higher number of households that had to be sampled in order to achieve the sample size of 2,000 households in phone-based CES compared in-person CES (3,600 and 2,352 respectively). Although the phone-based CES took less time to complete than in person coverage evaluations, the surveyors highlighted the lack of gender representation among phone survey participants as it was mostly men who answered calls and were then interviewed. The surveyors also mentioned that eliciting responses to open-ended questions and confirming treatment compliance from every member of the household was challenging during phone based CES. These observations were confirmed by analysing the survey participation data which showed women’s participation in CES was significantly lower in phone-based CES (66%) compared to in-person CES (94%) (Z = -22.38; p<0.01) and that a significantly higher proportion of households provided proxy responses in phone-based CES (51%) compared to in-person CES (21%) (Z = 20.23; p<0.01).

Conclusions

The phone-based CES may be a viable option to evaluate treatment coverage but issues such as participation bias, gender inclusion, and quality of responses will need to be addressed to optimize this methodology.
Songbird mesostriatal dopamine pathways are spatially segregated before the onset of vocal learning
Authors: Ramarao Malavika , Jones Caleb , Goldberg Jesse H. , Roeser Andrea
Published: November 2023

Diverse dopamine (DA) pathways send distinct reinforcement signals to different striatal regions. In adult songbirds, a DA pathway from the ventral tegmental area (VTA) to Area X, the striatal nucleus of the song system, carries singing-related performance error signals important for learning. Meanwhile, a parallel DA pathway to a medial striatal area (MST) arises from a distinct group of neighboring DA neurons that lack connectivity to song circuits and do not encode song error. To test if the structural and functional segregation of these two pathways depends on singing experience, we carried out anatomical studies early in development before the onset of song learning. We find that distinct VTA neurons project to either Area X or MST in juvenile birds before the onset of substantial vocal practice. Quantitative comparisons of early juveniles (30–35 days post hatch), late juveniles (60–65 dph), and adult (>90 dph) brains revealed an outsized expansion of Area X-projecting neurons relative to MST-projecting neurons in VTA over development. These results show that a mesostriatal DA system dedicated to social communication can exist and be spatially segregated before the onset of vocal practice and associated sensorimotor experience.
Tamarix articulata extract offers protection against toxicity induced by beauty products in Hs27 human skin fibroblasts
Authors: Alnuqaydan Abdullah M. , Zainy Faten M. Ali , Almutary Abdulmajeed G. , Sadier Najwane Said , Rah Bilal
Published: November 2023

The current study evaluates the cytotoxicity, mode of cell death and chemical analysis of selected beauty products and evaluation of the protective effect of Tamarix articulata (TA) extract against toxicity induced by beauty products in skin fibroblasts (Hs27). MTT and Crystal violet (CV) assays were used to determine the dose-dependent cytotoxic effects of beauty products against Hs27 fibroblasts. DNA fragmentation assay and annexin-V staining were conducted to determine the mode of cell killing induced by evaluated beauty products. Quantification of reactive oxygen species (ROS) and antioxidant enzyme levels were used to evaluate the oxidative stress. Chemical analysis and heavy metals were evaluated to determine beauty products. Pre-treatment with TA extract for different time points followed by time-dependent exposure with beauty products to assess the protective effect of TA extract in Hs27 cells was analyzed by MTT and CV assays. Owing to the presence of various harmful heavy metals such as arsenic (As), chromium (Cr), cadmium (Cd), nickel (Ni), and lead (Pb) in beauty products, our results revealed that all beauty products induce significant cytotoxicity over time (1, 4 h) in a dose-dependent (125, 250, 500 μg/mL) manner. DNA fragmentation assay, quantification of apoptosis by annexin-V staining, determination of ROS and antioxidant enzymes (CAT, GSH-Px and SOD) revealed that the induced cytotoxicity was caused by oxidative stress-mediated apoptosis. However, pre-incubation with a safe dose (50 μg/mL) of TA for different times (24, 48 h) followed by exposure to various doses (62.5, 125, 250, 500 μg/mL) of beauty products for different times (1, 4 h) revealed significant (*p≤0.05, **p≤0.01) protection against beauty product-mediated cytotoxicity. The effect was more pronounced for 1 h exposure to beauty products compared to 4 h. Our study demonstrates that the due to the presence of heavy metals in synthetic beauty products exhibit marked toxicity to skin fibroblasts due to oxidative stress-mediated apoptosis. However, the presence of abundant bioactive polyphenols with promising antiscavenging activity in TA extracts significantly nullifies cytotoxicity promoted by examined beauty products in skin fibroblasts (Hs27).
Induced interpretation bias affects free recall and episodic memory bias in social anxiety
Authors: Park Hye Ryeong , Lee Jong-Sun
Published: November 2023

The combined effect of each cognitive bias, interpretation, attention, and memory bias, is known to play a causal role in the etiology and maintenance of social anxiety. However, little is known about how each type of bias (i.e., interpretation, memory bias) acts during social anxiety. The present study aimed to investigate whether experimentally induced interpretation bias using the cognitive bias modification (CBM) paradigm would influence free recall and episodic memory biases in a Korean sample. A total of 61 participants were randomly assigned to either a positive (n = 30) or negative (n = 31) CBM group. The study used CBM scenarios that were auditory-specific and focused on social anxiety symptoms. The results showed that interpretation biases could be induced, and they resulted in training congruent state mood and memory biases on both free-recall memory and autobiographical memory, which partly confirmed the combined cognitive biases hypothesis proposed by Hirsch, Clark (1).
Effectivenes of incentive constraint policies in enhancing green bond credit rating and certification: A theoretical and empirical study
Authors: Zhao Hanyi , Tian Yixiang , Zhou Xiangyun , Zhao Xuefeng
Published: November 2023

This paper aims to effectively reduce CO2 emissions by examining the impact of three distinct incentive and constraint policies on the quality of rating and certification information in China’s green bond issuance market. To accomplish this, the government has implemented incentives, while regulators have introduced constraints to curb the spread of inflated rating and certification information. We build on the integrated rating and certification regulation mechanism by presenting a two-stage Stackelberg game model that involves four key participants: the China Securities Regulatory Commission, local governments, green evaluation and certification agencies, and credit rating agencies. We incorporate environmental effects indicators into the expected utility of rating and certification agencies to investigate the equilibrium conditions under three policy scenarios: a single financial incentive policy, a single regulatory constraint policy, and a combined incentive and constraint policy. The paper employs Stackelberg game theory to analyze how different policies mitigate the occurrence of “inflated” ratings and “greenwashing” in certifications. Numerical analysis is conducted to validate the theoretical findings. Moreover, we assess the impact of these policies on the quality of rating and evaluation information, using data from China’s green bond issuance market between 2016 and 2021. Our research offers valuable management insights and regulatory recommendations for both regulators and local governments.
An improved gene expression programming algorithm for function mining of map-reduce job execution in catenary monitoring systems
Authors: Ding Jin , Jiang Tianyu , Tan Ping , Wang Yi , Fei Zhenshun , Huang Chuyuan , Ma Jien , Fang Youtong
Published: November 2023

Gene expression programming (GEP) is one of the most prominent algorithms in function mining. In order to obtain a more accurate function model in configuration parameters-execution efficiency (CP-EE) of map-reduce job in the high-speed railway catenary monitoring system, this paper proposes a novel algorithm, called GEP based on multi-strategy (MS-GEP). Compared to traditional GEP, the proposed algorithm can escape premature convergence and jump out of local optimum. First, an adaptive mutation rate is designed according to the evolutionary generations, population diversity, and individual fitness values. A manual intervention strategy is then proposed to determine whether the algorithm enters the dilemma of local optimum based on the generations of population evolutionary stagnation. Finally, the average quality of the population is changed by randomly replacing individuals, and the ancestral population is traced to change the evolutionary direction. The experimental results on the benchmarks of function mining show that the proposed MS-GEP has better solution quality and higher population diversity than other GEP algorithms. Furthermore, the proposed MS-GEP has higher accuracy on the function model of CP-EE of high-speed railway catenary monitoring system than other commonly used algorithms in the field of function mining.
Mapping landscape ecological patterns using numeric and categorical maps
Authors: Riitters Kurt , Vogt Peter
Published: November 2023

The reciprocal relationships between ecological process and landscape pattern are fundamental to landscape ecology. Landscape ecologists traditionally use raster maps portraying classified features such as land use or land cover categories, and metrics suggested by the patch-corridor-matrix conceptual model of pattern. Less attention has been given to the landscape gradient conceptual model and raster maps portraying numeric features such as greenness or percent vegetation cover. We introduce the open-source tool GraySpatCon to calculate and map a variety of landscape pattern metrics from both conceptual models using either categorical or numeric maps. The 51 metrics, drawn mostly from the landscape ecology and image processing literatures, are calculated from the frequencies of input pixel values and/or the pixel value adjacencies in an analysis region. GraySpatCon conducts either a moving window analysis which produces a continuous map of a pattern metric, or a global analysis which produces a single metric value. We describe an implementation in the GuidosToolbox desktop application which allows novice users to interactively explore GraySpatCon functionality. In the R desktop environment, we demonstrate several metrics using an example map of percent tree cover and illustrate a multi-scale moving window analysis to identify scale domains. Comparisons of computational efficiency indicate a substantial GraySpatCon advantage over related software in the R environment.
Healthy adults’ views and experiences on behavior change strategies in mobile applications for diet monitoring: A single centre qualitative study
Authors: Abdul Khalil Nur Melissa , Mohd Mydin Fadzilah Hanum , Moy Foong Ming
Published: November 2023

Mobile diet apps assist in tracking nutritional intake and managing healthy eating diets. Effective diet apps incorporate specific population-tailored behavior change strategies (BCS) for user engagement and adherence to healthy diets. Malaysians have their unique behaviors and customs surrounding food and diet. This study aims to explore the perceptions, views, and experiences of healthy Malaysian adults with diet monitoring apps, by focusing on the BCS that engages users to use diet mobile apps and adhere to a healthy diet. A qualitative approach utilizing semi-structured in-depth interviews was conducted using a topic guide based on the Theory of Planned Behavior and trigger materials. Twenty interviews were audio-recorded and transcribed verbatim. Five themes emerged from the data, which are; instilling self-awareness, closed online group support, shaping knowledge, personalization, and user-friendly design. Influence by one’s social circle and attractiveness of app features may initiate users’ interest and help them engage with mobile diet apps, but the app’s ability to raise awareness of progress and impart useful knowledge help them adhere and comply to a healthier diet in the long run. The results from this study may help improve the behavior change strategy features of mobile diet apps for Malaysian adults.
Sustainability of facilities built under the Community-Led Total Sanitation (CLTS) implementation: Moving from basic to safe facilities on the sanitation ladder
Authors: Kouassi Hemez Ange Aurélien , Andrianisa Harinaivo Anderson , Sossou Seyram Kossi , Traoré Maïmouna Bologo , Nguematio Rikyelle Momo
Published: November 2023

In the context of monitoring progress towards SDG target 6.2, a household is counted to have access to sanitation if it uses at least basic sanitation services. Several approaches have been employed to help rural communities to climb up the sanitation ladder such as Community-led Total Sanitation (CLTS), whose primary target is to end open defecation through behavior change. CLTS does not subsidize sanitation facilities, but let households build their own facilities. The types and sustainability of facilities when construction is entrusted to households without guidelines remain understudied. The contribution of CLTS in achieving SDG6.2 also have not been studied. This paper addresses these gaps. Conducted in the province of Sissili in Burkina Faso, our study involved interviewing CLTS implementers, government officials, and community stakeholders. Coupled with household surveys, the data was analyzed using SPSS and Excel software. Findings indicate that CLTS succeeded in motivating households to build latrines hence escalating latrine coverage from 29.51% in 2016 (pre-CLTS) to 90.44% in 2020 (post-CLTS) in the province. However, 97.53% of latrines built were unimproved pit latrines with superstructures and without/with wooden or clay slabs and no roof, of which 19.76% collapsed during the rainy season. During this period, sanitation access rate rose from 11.9% to 17.00%. The study has therefore revealed that CLTS significantly elevates latrine coverage, yet it does not guarantee a proportional rise in sanitation access. This discrepancy results from the type of technologies generated by CLTS, which are not considered in calculating the sanitation access rate due to their unimproved nature. Consequently, further exploration of social approaches is essential, amalgamating technical and engineering aspects. Beyond socio-economic considerations, the sustainability of CLTS and the achievement of access to adequate and safe sanitation also rely on the robustness and resilience of the implemented facilities.
Treatment for radiographically active, sputum culture-negative pulmonary tuberculosis: A systematic review and meta-analysis
Authors: Gray Adam Thorburn , Macpherson Liana , Carlin Ffion , Sossen Bianca , Richards Alexandra S. , Kik Sandra V. , Houben Rein M. G. J. , MacPherson Peter , Quartagno Matteo , Rogozińska Ewelina , Esmail Hanif
Published: November 2023

Background

People with radiographic evidence for pulmonary tuberculosis (TB), but negative sputum cultures, have increased risk of developing culture-positive TB. Recent expansion of X-ray screening is leading to increased identification of this group. We set out to synthesise the evidence for treatment to prevent progression to culture-positive disease.

Methods

We conducted a systematic review and meta-analysis. We searched for prospective trials evaluating the efficacy of TB regimens against placebo, observation, or alternative regimens, for the treatment of adults and children with radiographic evidence of TB but culture-negative respiratory samples. Databases were searched up to 18 Oct 2022. Study quality was assessed using ROB 2·0 and ROBINS-I. The primary outcome was progression to culture-positive TB. Meta-analysis with a random effects model was conducted to estimate pooled efficacy. This study was registered with PROSPERO (CRD42021248486).

Findings

We included 13 trials (32,568 individuals) conducted between 1955 and 2018. Radiographic and bacteriological criteria for inclusion varied. 19·1% to 57·9% of participants with active x-ray changes and no treatment progressed to culture-positive disease. Progression was reduced with any treatment (6 studies, risk ratio [RR] 0·27, 95%CI 0·13–0·56), although multi-drug TB treatment (RR 0·11, 95%CI 0·05–0·23) was significantly more effective than isoniazid treatment (RR 0·63, 95%CI 0·35–1·13) (p = 0·0002).

Interpretation

Multi-drug regimens were associated with significantly reduced risk of progression to TB disease for individuals with radiographically apparent, but culture-negative TB. However, most studies were old, conducted prior to the HIV epidemic and with outdated regimens. New clinical trials are required to identify the optimal treatment approach.
Oxytocinergic projection from the hypothalamus to supramammillary nucleus drives recognition memory in mice
Authors: Takahashi Junpei , Yamada Daisuke , Nagano Wakana , Sano Yoshitake , Furuichi Teiichi , Saitoh Akiyoshi
Published: November 2023

Oxytocin (OXT) neurons project to various brain regions and its receptor expression is widely distributed. Although it has been reported that OXT administration affects cognitive function, it is unclear how endogenous OXT plays roles in cognitive function. The present study examined the role of endogenous OXT in mice cognitive function. OXT neurons were specifically activated by OXT neuron-specific excitatory Designer Receptors Exclusively Activated by Designer Drug expression system and following administration of clozapine-N-oxide (CNO). Object recognition memory was assessed with the novel object recognition task (NORT). Moreover, we observed the expression of c-Fos via immunohistochemical staining to confirm neuronal activity. In NORT, the novel object exploration time percentage significantly increased in CNO-treated mice. CNO-treated mice showed a significant increase in the number of c-Fos-positive cells in the supramammillary nucleus (SuM). In addition, we found that the OXT-positive fibers from paraventricular hypothalamic nucleus (PVN) were identified in the SuM. Furthermore, mice injected locally with CNO into the SuM to activate OXTergic axons projecting from the PVN to the SuM showed significantly increased percentage time of novel object exploration. Taken together, we proposed that object recognition memory in mice could be modulated by OXT neurons in the PVN projecting to the SuM.
Supporting clinical decision making in the emergency department for paediatric patients using machine learning: A scoping review protocol
Authors: Leonard Fiona , O’Sullivan Dympna , Gilligan John , O’Shea Nicola , Barrett Michael J.
Published: November 2023

Introduction

Machine learning as a clinical decision support system tool has the potential to assist clinicians who must make complex and accurate medical decisions in fast paced environments such as the emergency department. This paper presents a protocol for a scoping review, with the objective of summarising the existing research on machine learning clinical decision support system tools in the emergency department, focusing on models that can be used for paediatric patients, where a knowledge gap exists.

Materials and methods

The methodology used will follow the scoping study framework of Arksey and O’Malley, along with other guidelines. Machine learning clinical decision support system tools for any outcome and population (paediatric/adult/mixed) for use in the emergency department will be included. Articles such as grey literature, letters, pre-prints, editorials, scoping/literature/narrative reviews, non-English full text papers, protocols, surveys, abstract or full text not available and models based on synthesised data will be excluded. Articles from the last five years will be included. Four databases will be searched: Medline (EBSCO), CINAHL (EBSCO), EMBASE and Cochrane Central. Independent reviewers will perform the screening in two sequential stages (stage 1: clinician expertise and stage 2: computer science expertise), disagreements will be resolved by discussion. Data relevant to the research question will be collected. Quantitative analysis will be performed to generate the results.

Discussion

The study results will summarise the existing research on machine learning clinical decision support tools in the emergency department, focusing on models that can be used for paediatric patients. This holds the promise to identify opportunities to both incorporate models in clinical practice and to develop future models by utilising reviewers from diverse backgrounds and relevant expertise.
Efficacy and safety of vitamin D supplementation on psoriasis: A systematic review and meta-analysis
Authors: Dai Qianqian , Zhang Yanfeng , Liu Qian , Zhang Chijin
Published: November 2023

Objectives

Our aim was to analyze the results of published randomized controlled trials (RCTs) on vitamin D supplementation for psoriasis in order to explore its effectiveness and safety.

Patients and methods

As of July 7 2023, we conducted a systematic literature search in PubMed, Cochrane, Embase, and Web of Science Core Collection databases. The study outcomes included change values in Psoriasis Area and Severity Index (PASI) (at 3 months, 6 months, and end of follow-up)/Dermatology Life Quality Index (DLQI)/Psoriasis disability index (PDI)/C-reactive protein (CRP), and adverse events.

Results

333 patients from 4 studies were evaluated. Pooled analyses showed no significant effect of DLQI/PDI/CRP change value (P > 0.05) or PASI change value (3 months, end of follow-up; P > 0.05). Sensitivity analyses and statistical tests did not support the results of the PASI change values (6 months, P = 0.05). However, the results of subgroup analyses should not be ignored(supplementation with vitamin D2 or Asia would be more effective; P = 0.03). There were no serious adverse effects, and only a few individuals experienced nausea.

Conclusions

The efficacy and safety of vitamin D supplementation in the treatment of psoriasis remains unremarkable. The search for a new prognostic index that combines clinical and laboratory factors is needed to compensate for the shortcomings of existing measures and provide stronger evidence of validity.
An empirical study on learners’ learning emotion and learning effect in offline learning environment
Authors: Mou Xiangwei , Xin Yu , Song Yongfu , Xiang Jinshan , Tang Yuanbin
Published: November 2023

The application of non-cognitive factors represented by facial emotion in educational evaluation has attracted much attention in recent years. There are many existing studies on facial emotion assisted education evaluation, but most of them are based on virtual learning environments, which means that the research on facial emotion and learning effect in offline learning environments is sparse. In order to solve this problem, this study designed an emotion observation experiment based on the offline learning environment, obtained the type of learner facial emotion and learning effect of 127 college students, and further explored the relationship between the two. The results show that: 1) We obtained eight types of learner emotion through the combined description method: joy, relaxation, surprise, meekness, contempt, disgust, sadness, anxiety and their respective PAD emotional mean. 2) We obtained the correlation results of the six emotions of joy, relaxation, surprise, meekness, contempt, and anxiety with the learning effect and the predicted value of the learning effect. 3) We then constructed an explanatory model of learner emotion and learning effect based on the offline learning environment.
Clinical outcomes of immunoglobulin treatment for patients with secondary antibody deficiency: Data from the Ontario immunoglobulin treatment case registry
Authors: Abadeh Armin , Shehadeh Sarah , Betschel Stephen , Waserman Susan , Cameron Donald William , Cowan Juthaporn
Published: November 2023

Background

Despite the increasing number of cases of secondary antibody deficiency (SAD) and immunoglobulin (Ig) utilization, there is a paucity of data in the literature on clinical and patient-reported outcomes in this population.

Objective

To describe immunoglobulin utilization patterns, clinical and patient-reported outcomes in patients with SAD on immunoglobulin replacement therapy (IgRT).

Methods

A cross-sectional study of patients with secondary antibody deficiency enrolled in the Ontario Immunoglobulin Treatment (ONIT) Case Registry from June 2020 to September 2022 was completed. Demographics, comorbidities, indications for immunoglobulin treatment, clinical infections at baseline and post IgRT, and patient-reported outcomes were collected and analyzed.

Results

There were 140 patients (58 males; 82 females; median age 68) with SAD during the study period; 131 were on subcutaneous Ig (SCIG) and 9 were on intravenous Ig (IVIG). The most common indication was chronic lymphocytic leukemia (CLL) (N = 52). IgRT reduced the average annual number of infections by 82.6%, emergency room (ER) visits by 84.6%, and hospitalizations by 83.3%. Overall, 84.6% of patients reported their health as better compared to before IgRT. Among those patients who switched from IVIG to SCIG (N = 35), 33.3% reported their health as the same, and 62.9% reported their health as better.

Conclusions

This study demonstrates that IgRT significantly improved clinical outcomes and patient-reported general health state in patients with SAD. This study also further supports the use of SCIG in patients with SAD.
Prevalence and clinical implications of respiratory viruses in asthma during stable disease state and acute attacks: Protocol for a meta-analysis
Authors: Alimani Gioulinta S. , Ananth Sachin , Boccabella Cristina , Khaleva Ekaterina , Roberts Graham , Papadopoulos Nikolaos G. , Kosmidis Chris , Vestbo Jørgen , Papageorgiou Effie , Beloukas Apostolos , Mathioudakis Alexander G.
Published: November 2023

Introduction

Viruses are detected in over 50% of acute asthma attacks and in a notable proportion of patients with asthma during stable disease state They are associated with worse outcomes. We will conduct a series of systematic reviews and meta-analyses to quantify the prevalence and clinical burden of various respiratory viruses in stable asthma and acute asthma attacks. In addition, we will assess the viral loads of respiratory viruses during stable and acute asthma, to explore whether viral load could differentiate attacks triggered by viruses versus those where viruses are present as “innocent bystanders”.

Materials and methods

Based on a prospectively registered protocol (PROSPERO, ID: CRD42023375108) and following standard methodology recommended by Cochrane, we will systematically search Medline/PubMed, EMBASE, the Cochrane Library and relevant conference proceedings for studies assessing the prevalence or clinical burden of respiratory viruses in asthma. Methodological rigour of the included studies will be appraised using a tool specific for prevalence studies and the Newcastle-Ottawa Scale respectively. In anticipation of significant clinical and methodological heterogeneity, we will conduct random effect meta-analyses. For evaluating the prevalence of viruses, we will perform meta-analyses of proportions using the inverse variance method, and the Freeman-Tukey transformation. We will conduct meta-regression analyses for exploring heterogeneity.

Conclusion

We envisage that these systematic reviews and meta-analyses will quantify the prevalence and burden of respiratory viruses in stable and acute asthma and will drive future research and clinical practice.
Dysfunction of peripheral somatic and autonomic nervous system in patients with severe forms of Crohn’s disease on biological therapy with TNFα inhibitors–A single center study
Authors: Wasserbauer Martin , Mala Sarka , Stechova Katerina , Hlava Stepan , Cernikova Pavlina , Stovicek Jan , Drabek Jiri , Broz Jan , Pichlerova Dita , Kucerova Barbora , Liskova Petra , Kral Jan , Bartuskova Lucia , Keil Radan
Published: November 2023

Objective

Crohn’s disease (CD) can be associated with a wide range of extraintestinal manifestations (EIMs), including neurological ones. Published studies differ in their conclusions about the epidemiology and etiopathogenesis of neurological EIMs. The aims of this study were to demonstrate the presence and find risk factors of peripheral (somatic and autonomic) neuropathy patients with severe CD on anti-TNFα biological therapy.

Material and methods

A clinical examination focusing on detection of peripheral sensor-motor nervous dysfunction (including Sudoscan) and examination of autonomic nervous system dysfunction (using Ewing´s battery tests and spectral analysis) together with laboratory tests and collection of demographic data followed by administration of questionnaires were performed on a total of 30 neurologically asymptomatic outpatients with severe CD on anti-TNFα biological therapy.

Results

Peripheral sensor-motor nervous function via clinical neurological examination was pathological in 36.7% and Sudoscan in 33.3% of cases. Statistically significant associations between vibration perception test and age, CD and biological therapy duration, body mass index and Crohn’s Disease Activity Index were proved while statistically significant associations between temperature perception test and age and BMI were proved as well. Additionally, a decrease of total protein in a patient´s serum below the physiological cut-off in the 6 months prior to measurement was associated with a pathological result of a Sudoscan. Cardiovascular autonomic neuropathy based on Ewing´s battery tests was present in 56.7% of patients, no statistically significant risk factors were found. Our peripheral neuropathy questionnaire correlated with the results of the Sudoscan test and some tests of the clinical examination of peripheral sensor-motor nervous function (discriminatory contact perception test, temperature perception test).

Conclusions

This study demonstrated a relatively high prevalence of peripheral (especially autonomic) neuropathy and verified some risk factors for the development of peripheral somatic neuropathy in asymptomatic patients with severe form of CD on anti-TNFα biological therapy.
Differences in the genomic diversity, structure, and inbreeding patterns in wild and managed populations of Agave potatorum Zucc. used in the production of Tobalá mezcal in Southern Mexico
Authors: Ruiz Mondragón Karen Y. , Klimova Anastasia , Aguirre-Planter Erika , Valiente-Banuet Alfonso , Lira Rafael , Sanchez-de la Vega Guillermo , Eguiarte Luis E.
Published: November 2023

Agave potatorumZucc. locally known as Tobalá, is an important species for mezcal production. It is a perennial species that takes 10 to 15 years to reach reproductive age. Because of high demand of Tobalá mezcal and the slow maturation of the plants, its wild populations have been under intense anthropogenic pressure. The main objective of this study was to estimate the genome-wide diversity inA.potatorumand determine if the type of management has had any effect on its diversity, inbreeding and structure. We analyzed 174 individuals (105 wild, 42 cultivated and 27 from nurseries) from 34 sites with a reduced representation genomic method (ddRADseq), using 14,875 SNPs. The diversity measured as expected heterozygosity was higher in the nursery and wild plants than in cultivated samples. We did not find private alleles in the cultivated and nursery plants, which indicates that the individuals under management recently derived from wild populations, which was supported by higher gene flow estimated from wild populations to the managed plants. We found low but positive levels of inbreeding (FIS= 0.082), probably related to isolation of the populations. We detected low genetic differentiation among populations (FST= 0.0796), with positive and significant isolation by distance. The population genetic structure in the species seems to be related to elevation and ecology, with higher gene flow among populations in less fragmented areas. We detected an outlier locus related to the recognition of pollen, which is also relevant to self-incompatibility protein (SI). Due to seed harvest and long generation time, the loss of diversity inA.potatorumhas been gradual and artificial selection and incipient management have not yet caused drastic differences between cultivated and wild plants. Also, we described an agroecological alternative to the uncontrolled extraction of wild individuals.
Study of heavy metal resistance genes in Escherichia coli isolates from a marine ecosystem with a history of environmental pollution (arsenic, cadmium, copper, and mercury)
Authors: Tseng Ashley S. , Roberts Marilyn C. , Weissman Scott J. , Rabinowitz Peter M.
Published: November 2023

We analyzed whole genome sequences of 308 Escherichia coli isolates from a marine ecosystem to determine the prevalence and relationships of heavy metal resistance genes (HMRGs) and antibiotic resistance genes (ARGs), as well as the presence of plasmid sequences. We screened all genomes for presence of 18 functional HMRGs conferring resistance to arsenic, cadmium, copper, or cadmium/mercury. In subset analyses, we examined geographic variations of HMRG carriage patterns in 224 isolates from water sources, and sought genetic linkages between HMRGs and ARGs in 25 genomes of isolates resistant to antibiotics. We found high carriage rates of HMRGs in all genomes, with 100% carrying at least one copy of 11 out of 18 HMRGs. A total of 173 (56%) of the isolates carried both HMRGs and plasmid sequences. In the 25 genomes of antibiotic-resistant isolates, 80% (n = 20) carried HMRGs, ARGs, and plasmid sequences, while 40% (n = 10) had linked HMRGs and ARGs on their assembled genomes. We found no evidence of geographic variation in HMRG frequency, nor any association between locational proximity to Superfund sites and co-carriage of HMRGs and ARGs. Our study findings indicate that HMRGs are common among E. coli in marine ecosystems, suggesting widespread heavy metal presence in water sources of a region with history of environmental pollution. Further research is needed to determine the role HMRGs play in driving antimicrobial resistance in human pathogens through genetic linkage and the value their detection in environmental bacterial genomes may offer as an indicator of environmental heavy metal pollution.