bluuebunny/crossref_metadata_2025 huggingface dataset:
Driving factors and key paths of greenhouse gas and air pollutants synergistic control using hybrid multiple-criteria decision-making
Authors: Guo Di , Li Yingqiu , Xie Danlan , Sun Dongshi
Published: April 2024

Humanity has consumed a large amount of energy and resources to maintain the rapid development of the economy and society, causing greenhouse gas and air pollutants to rise continuously, generating enormous pressures for the sustainable development of many cities. It is economical to control greenhouse gas and air pollutants from the synergy perspective. To identify the key driving factors involved in synergistic control, this paper uses the pressure-state-response (PSR) model to design a performance evaluation model of greenhouse gas and air pollutants synergistic control (GASC) utilizing pressure, state, and response dimensions. The performance evaluation factor system of GASC comprises three primary aspects and 18 criteria. The analytic hierarchy process (AHP) was used to determine the weight of each factor in the evaluation system. The technique for order preference by similarity to an ideal solution (TOPSIS) method was used to calculate the ranking of the synergistic control effects of the four representative provinces in China. We use Importance-performance analysis (IPA) to analyze the performance of driving factors of synergistic control in the province with the lowest ranking from 2016 to 2020. The research shows that in Northeast China, represented by Liaoning province, the government’s response should include changing the support strategy for the new energy consumer, introducing synergistic control standards and policies, and making flexible adjustments to the supply chain. The research provides a scientific basis for the performance evaluation of GASC and decision-making support for lean response strategies.
Research on the spatial spillover effect of carbon trading market development on regional emission reduction
Authors: Cui Yuxin , Feng Wei , Gu Xuesong
Published: April 2024

This paper introduces the current situation of carbon trading market in China and the effect of carbon emission reduction in each region. Theoretically, it expounds the influence mechanism and spatial spillover way of carbon trading market on regional carbon emission. Next, we use the data of 30 provinces in China from 2006 to 2019 to build a continuous spatial difference in difference model (SDM-DID) to empirically study the spatial spillover effect of carbon trading market on regional emission reduction. The results are as follows: First, the implementation of the pilot policy of carbon emission trading has significantly promoted the carbon emission reduction in the pilot areas. From the perspective of impact mechanism, the implementation of carbon trading pilot policy promotes carbon emission reduction in pilot areas by promoting clean transformation of energy consumption structure, improving technology absorption capacity and stimulating development of low-carbon technologies. From the perspective of spatial spillover effect, China’s regional carbon emissions have significant spatial spillover effect, and carbon emissions trading has spatial spillover effect. From the perspective of spatial spillover, the carbon emission trading market promotes the carbon emission reduction in neighboring areas by promoting the clean transformation of energy structure in neighboring areas, improving technology absorption capacity and promoting technological progress.
Corporate carbon emissions management and the disclosure of key audit matters
Authors: Wang Guoqing , Wu Maoguo
Published: April 2024

The principle that sustainable development can foster high-quality corporate growth has gained popularity as a tool for environmental protection. Utilizing data of firms listed on Shanghai Stock Exchange and Shenzhen Stock Exchange from 2010 to 2020, this study investigates the impact of corporate carbon emissions management (CCEM) on the disclosure of key audit matters (KAMs). The findings indicate that CCEM can significantly elevate the adequacy of disclosure concerning KAMs. Tests on underlying mechanism underscore that accounting conservatism and audit quality serve as pivotal channels through which CCEM facilitates the disclosure of KAMs. Further analysis on economic consequences reveals that the disclosure of KAMs helps mitigate stock price crash risk while enhancing environmental, social, and governance performance. This study enriches research on factors influencing the disclosure of KAMs, concurrently providing valuable insights for advancing high-quality corporate development and facilitating communication between auditors and users of audit reports.
The ins and outs of microglial cells in brain health and disease
Authors: Pallarés-Moratalla Carla , Bergers Gabriele
Published: April 2024

Microglia are the brain’s resident macrophages that play pivotal roles in immune surveillance and maintaining homeostasis of the Central Nervous System (CNS). Microglia are functionally implicated in various cerebrovascular diseases, including stroke, aneurysm, and tumorigenesis as they regulate neuroinflammatory responses and tissue repair processes. Here, we review the manifold functions of microglia in the brain under physiological and pathological conditions, primarily focusing on the implication of microglia in glioma propagation and progression. We further review the current status of therapies targeting microglial cells, including their re-education, depletion, and re-population approaches as therapeutic options to improve patient outcomes for various neurological and neuroinflammatory disorders, including cancer.
Concomitant use of interleukin-2 and tacrolimus suppresses follicular helper T cell proportion and exerts therapeutic effect against lupus nephritis in systemic lupus erythematosus-like chronic graft versus host disease
Authors: Nasa Yutaro , Satake Atsushi , Tsuji Ryohei , Saito Ryo , Tsubokura Yukie , Yoshimura Hideaki , Ito Tomoki
Published: April 2024

Introduction

Defective interleukin-2 (IL-2) production contributes to immune system imbalance in patients with systemic erythematosus lupus (SLE). Recent clinical studies suggested that low-dose IL-2 treatment is beneficial for SLE and the therapeutic effect is associated with regulatory T cell (Treg) expansion. Pharmacological calcineurin inhibition induces a reduction in the number of Tregs because they require stimulation of T cell receptor signaling and IL-2 for optimal proliferation. However, the activation of T cell receptor signaling is partially dispensable for the expansion of Tregs, but not for that of conventional T cells if IL-2 is present.

Aim

We examined whether addition of IL-2 restores the Treg proportion even with concurrent use of a calcineurin inhibitor and if the follicular helper T cell (Tfh) proportion is reduced in an SLE-like murine chronic graft versus host disease model.

Methods

Using a parent-into-F1 model, we investigated the effect of IL-2 plus tacrolimus on Treg and Tfh proportions and the therapeutic effect.

Results

Treatment with a combination of IL-2 and tacrolimus significantly delayed the initiation of proteinuria and decreased the urinary protein concentration, whereas tacrolimus or IL-2 monotherapy did not significantly attenuate proteinuria. Phosphorylation of signal transducer and activator of transcription 3, a positive regulator of Tfh differentiation, was reduced by combination treatment, whereas phosphorylation of signal transducer and activator of transcription 5, a negative regulator, was not reduced.

Conclusion

Addition of calcineurin inhibitors as adjunct agents may be beneficial for IL-2-based treatment of lupus nephritis.
Case report: Atypical case of autoimmune glial fibrillary acidic protein astrocytopathy following COVID-19 vaccination refractory to immunosuppressive treatments
Authors: Morishima Yuto , Hata Takanori , Nakajima Sho , Shindo Kazumasa , Tsuchiya Mai , Watanabe Tsubasa , Tahara Ippei , Kondo Tetsuo , Kimura Akio , Shimohata Takayoshi , Ueno Yuji
Published: April 2024

A 54-year-old Japanese man presented with headache and fever the day after SARS-CoV-2 vaccination. He became deeply unconscious within a week. Brain MRI showed periventricular linear enhancements and a few spotty lesions in the cerebral white matter. Cerebrospinal fluid (CSF) testing showed mild pleocytosis. He was treated with intravenous methylprednisolone and plasma exchange. However, the white matter lesions enlarged to involve the brainstem and cerebellum, and long cord spinal lesions appeared. Anti-glial fibrillary acidic protein (GFAP) antibody was positive in the CSF and serum, and he was therefore diagnosed as autoimmune GFAP-astrocytopathy (GFAP-A). In addition, high-dose immunoglobulin therapy was administered twice, but his symptoms did not improve; the white matter lesions enlarged further, and modified Rankin Scale score increased to 5. A brain biopsy specimen showed infiltration of macrophages and CD4+ lymphocytes together with neuron and oligodendrocytic injuries and glial scar. Although GFAP-A generally responds well to steroids, the present case developed GFAP-A following SARS-CoV-2 vaccination, with refractory to intensive immunosuppressive therapy and atypical pathologic findings of infiltration of CD4+ lymphocytes and demyelination.
Creatinine-to-cystatin C ratio and body composition predict response to PD-1 inhibitors-based combination treatment in metastatic gastric cancer
Authors: Ji Hongjuan , Liu Bona , Jin Peng , Li Yingchun , Cui Lili , Jin Shanxiu , Wu Jingran , Shan Yongqi , Zhang Zhenyong , Ming Jian , Zhang Liang , Du Cheng
Published: April 2024

Background

Creatinine-to-cystatin C ratio (CCR) and body composition (BC) parameters have emerged as significant prognostic factors in cancer patients. However, the potential effects of CCR in gastric cancer (GC) remains to be elucidated. This multi-center retrospective study explored the predictive and prognostic value of CCR and BC-parameters in patients with metastatic GC receiving PD-1 inhibitors-based combination therapy.

Methods

One hundred and thirteen GC patients undergoing PD-1 inhibitors-based combination therapy were enrolled at three academic medical centers from January 2021 to July 2023. A deep-learning platform based on U-Net was developed to automatically segment skeletal muscle index (SMI), subcutaneous adipose tissue index (SATI) and visceral adipose tissue index (VATI). Patients were divided into two groups based on the median of CCR or the upper tertile of BC-parameters. Logistic and Cox regression analysis were used to determine the effect of CCR and BC-parameters in predicting response rates and survival rates.

Results

The CCR was positively correlated with SMI (r=0.43; P<0.001), but not with SATI or VATI (P>0.05). Multivariable logistic analysis identified that both low CCR (OR=0.423, P=0.066 for ORR; OR=0.026, P=0.005 for DCR) and low SATI (OR=0.270, P=0.020 for ORR; OR=0.149, P=0.056 for DCR) were independently associated with worse objective response rate (ORR) and disease control rate (DCR). Patients with low CCR or low SATI had significantly lower 8-month progression-free survival (PFS) rate and 16-month overall survival (OS) rate than those with high CCR (PFS rate, 37.6% vs. 55.1%, P=0.011; OS rate, 19.4% vs. 44.9%, P=0.002) or those with high SATI (PFS rate, 37.2% vs. 53.8%, P=0.035; OS rate, 8.0% vs. 36.0%, P<0.001). Multivariate Cox analysis showed that low CCR (HR=2.395, 95% CI: 1.234-4.648, P=0.010 for PFS rate; HR=2.528, 95% CI: 1.317-4.854, P=0.005 for OS rate) and low SATI (HR=2.188, 95% CI: 1.050-4.560, P=0.037 for PFS rate; HR=2.818, 95% CI: 1.381-5.752, P=0.004 for OS rate) were both independent prognostic factors of poor 8-month PFS rate and 16-month OS rate. A nomogram based on CCR and BC-parameters showed a good performance in predicting the 12- and 16-month OS, with a concordance index of 0.756 (95% CI, 0.722-0.789).

Conclusions

Low pre-treatment CCR and SATI were independently associated with lower response rates and worse survival in patients with metastatic GC receiving PD-1 inhibitors-based combination therapy.
CAR products from novel sources: a new avenue for the breakthrough in cancer immunotherapy
Authors: Huang Jiawen , Yang Qian , Wang Wen , Huang Juan
Published: April 2024

Chimeric antigen receptor (CAR) T cell therapy has transformed cancer immunotherapy. However, significant challenges limit its application beyond B cell-driven malignancies, including limited clinical efficacy, high toxicity, and complex autologous cell product manufacturing. Despite efforts to improve CAR T cell therapy outcomes, there is a growing interest in utilizing alternative immune cells to develop CAR cells. These immune cells offer several advantages, such as major histocompatibility complex (MHC)-independent function, tumor microenvironment (TME) modulation, and increased tissue infiltration capabilities. Currently, CAR products from various T cell subtypes, innate immune cells, hematopoietic progenitor cells, and even exosomes are being explored. These CAR products often show enhanced antitumor efficacy, diminished toxicity, and superior tumor penetration. With these benefits in mind, numerous clinical trials are underway to access the potential of these innovative CAR cells. This review aims to thoroughly examine the advantages, challenges, and existing insights on these new CAR products in cancer treatment.
Kikuchi-Fujimoto disease evolves into lupus encephalopathy characterized by venous sinus thrombosis: a case report
Authors: Qin Wenyi , Yang Shuangshuang , Zhang Lijuan , Liu Mengqi , Tian Jiayu , Yang Juan , Zhou Guoqing , Rong Xiaofeng
Published: April 2024

Kikuchi-Fujimoto disease (KFD) is a benign, self-limiting illness that can progress to systemic lupus erythematosus (SLE) in approximately 30% of cases. Neurological injuries can occur in both diseases, albeit with distinct presentations. Venous sinus thrombosis is a serious cerebrovascular complication in patients with neuropsychiatric SLE but is rarely observed in patients with KFD. The involvement of various antibodies, particularly antiphospholipid antibodies, can cause vascular endothelial cell injury, resulting in focal cerebral ischemia and intracranial vascular embolism in SLE. However, there are cases in which thrombotic pathology occurs without antiphospholipid antibody positivity, attributed to vascular lesions. In this report, we present a case of KFD and lupus encephalopathy featuring cerebral venous sinus thrombosis, despite the patient being negative for antiphospholipid antibody. We also conducted a comparative analysis of C3 and C4 levels in cerebrospinal fluid (CSF) and peripheral blood, along with the protein ratio in CSF and serum, to elucidate the pathological changes and characteristics of lupus encephalopathy.
Multisensor data fusion of operational sea ice observations
Authors: Wang Keguang , Wang Caixin , Dinessen Frode , Spreen Gunnar , Ricker Robert , Tian-Kunze Xiangshan
Published: April 2024

Multisensor data fusion (MDF) is a process/technique of combining observations from multiple sensors to provide a more robust, accurate and complete description of the concerned object, environment or process. In this paper we introduce a new MDF method, multisensor optimal data fusion (MODF), to fuse different operational sea ice observations around Svalbard. The overall MODF includes regridding, univariate multisensor optimal data merging (MODM), multivariate check of consistency, and generation of new variables. For MODF of operational sea ice observations around Svalbard, the AMSR2 sea ice concentration (SIC) is firstly merged with the Norwegian Meteorological Institute ice chart. Then the daily SMOS sea ice thickness (SIT) is merged with the weekly CS2SMOS SIT to form a daily CS2SMOS SIT, which is further refined to be consistent with the SIC through consistency check. Finally sea ice volume (SIV) and its uncertainty are calculated based on the merged SIC and fused SIT. The fused products provide an improved, united, consistent and multifaceted description for the operational sea ice observations, they also provide consistent descriptions of sea ice edge and marginal ice zone. We note that uncertainties may vary during the regridding process, and therefore correct determination of the observation uncertainties is critically important for MDF. This study provides a basic framework for managing multivariate multisensor observations.
Stainless steel selection tool for water application: pitting engineering diagrams
Authors: Hägg Mameng Sukanya , Wegrelius Lena , Hosseinpour Saman
Published: April 2024

Introduction: This work systematically investigates the effect of chloride level, temperature, and the water system’s oxidative power on the pitting corrosion performance of stainless steels in pH-neutral environments.

Methods: Two test programs were set to a) develop a robust method for constructing the pitting engineering diagrams and b) construct the pitting engineering diagrams based on the obtained method from the first test program. The various electrochemical techniques were selected to assess and understand factors that affect the corrosion behavior of stainless steel. Extensive testing was performed with short-term electrochemical measurements and long-term immersion tests.

Results and Discussion: The obtained results demonstrate that the electrochemical methods are sufficient to define pitting diagrams showing the boundaries between pitting and no pitting as a function of chloride concentration, temperature, and the water system’s oxidation potential. The laboratory long-term electrochemical test results correspond the best to real applications and clearly underline the importance of an induction time for pit initiation. Accordingly, two sets of pitting engineering diagrams were constructed based on the water system’s oxidation potential. Measurements at the applied potential of 150 mV vs. saturated calomel electrode (SCE) correspond to applications in sterile tap water, whereas the applied potential of 400 mV vs. SCE corresponds to slightly chlorinated water or water with some biological activity. Pitting engineering diagrams were proved to be very useful tools to aid material selection in water application. However, it is important to realize that additional factors, such as different surface conditions and the presence of other environmental species, crevice design, or weld will affect the exact position of the boundaries between pitting and no pitting.
Antibacterial and anti-inflammatory polyethyleneimine/polyacrylic acid hydrogel loaded with CeO2 quantum dots for wet tissue wound dressings
Authors: Hou Wenxue , Lin Zehui , Xia Xiaomin , Sun Sa , Niu Zhaojun , Liu Jie , Lu Jiqing , Yin Dongming , Li Xue
Published: April 2024

Searching for an antibacterial and anti-inflammatory dressing that can stably adhere to wet tissues remains a momentous clinical challenge, especially in the context of treatment failure due to multi-drug-resistant bacteria. Using a hard template method in combination with an in situ chelating strategy, three-dimensional nitrogen-doped graded porous carbon anchored 1.5–2.5 nm CeO2 quantum dots (CeO2-QDs) were tailor-designed in this study. Using the size effect, CeO2-QDs have a higher percentage of Ce3+ and oxygen vacancies that could amplify their antibacterial effects. Polyethyleneimine/polyacrylic acid (PEA) powder could self-gel and be adhesive due to its strong physical interactions, which make it an ideal carrier for CeO2-QDs. PEA@50 (mg/mL) CeO2-QDs hydrogel and PEA@75 (mg/mL) CeO2-QDs hydrogel with moderate doses of CeO2-QDs show a superior antibacterial effect against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) strains. Furthermore, PEA@50CeO2-QDs hydrogels possess excellent anti-inflammatory capacity through their antioxidant activity, which could promote macrophage M2 phenotype polarization. More importantly, cytotoxicity assays on L929 fibroblasts show that PEA@CeO2-QDs hydrogels have no significant toxicity, and a significant proliferative effect could be observed. Overall, PEA@50CeO2-QDs hydrogels have the potential to become a multifunctional wet tissue dressing with anti-inflammatory and antiseptic properties to promote the healing of infected wounds.
Innovative framework for effective service parts management in the automotive industry
Authors: Nathan B. Sendhil , Siva Reddy B. Veera , Sastry C. Chandrasekhara , Krishnaiah J. , Eswaramoorthy K. V.
Published: April 2024

Effective service parts management and demand forecasting are crucial for optimizing operations in the automotive industry. However, existing literature lacks a comprehensive framework tailored to the specific context of the Thai automotive sector. This study addresses this gap by proposing a strategic approach to service parts management and demand forecasting in the Thai automotive industry. Drawing on a diverse set of methodologies, including classical time series models and advanced machine learning techniques, various forecasting models were assessed to identify the most effective approach for predicting service parts demand. Categorization of service parts based on demand criteria was conducted, and decision rules were developed to guide stocking strategies, balancing the need to minimize service disruptions with cost optimization. This analysis reveals substantial cost savings potential through strategic stocking guided by the developed decision rules. Furthermore, evaluation of the performance of different forecasting models recommends the adoption of Support Vector Regressor (SVR) as the most accurate model for forecasting service parts demand in this context. This research contributes to the automotive service industry by providing a nuanced framework for service parts management and demand forecasting, leading to cost-effective operations and enhanced service quality. The findings offer valuable insights for practitioners and policymakers seeking to improve efficiency and sustainability in the Thai automotive sector.
Case report: Iatrogenic tattoos caused by skin marking pen in a postoperative patient
Authors: Zhao Hanxing , Wu Xingru , Yu Yue , Cao Chang
Published: April 2024

In this report, a female patient suffering from pigment retention caused by a skin marking pen was elucidated. The patient underwent blepharoplasty 6 months ago and presented with blue-black linear marks at the upper eyelid incision 2 weeks after surgery. Under dermoscopy, scattered pigments were observed to accumulate in the epidermis of the upper eyelid. The patient was diagnosed with iatrogenic tattoo by a surgical marking pen. We chose surgical excision of the skin with the pigmentation. Previous studies have established that the risk of bacterial contamination, contact dermatitis, and allergies may increase with the surgical marking pens, while pigment retention has not yet been mentioned yet. Here, we present a case with a pigment retention in the incision. The selection of the surgical labelling methods and the management of the pigmentation were also addressed. According to our clinical findings, the risk of pigment retention by marking pens needs to be mentioned in the patient’s informed consent. Therefore, the practitioner should ensure that the ink is cleaned by the end of each invasive procedure.
Characterization of the disease-causing mechanism of KIF3B mutations from ciliopathy patients
Authors: Adams Jessica M. , Sawe Caleb , Rogers Skye , Reid Jordyn , Dasari Ronith , Engelke Martin F.
Published: April 2024

The heterodimeric kinesin-2 motor (KIF3A/KIF3B with accessory protein KAP3) drives intraflagellar transport, essential for ciliogenesis and ciliary function. Three point mutations in the KIF3B subunit have recently been linked to disease in humans (E250Q and L523P) and Bengal cats (A334T) (Cogné et al., Am. J. Hum. Genet., 2020, 106, 893–904). Patients display retinal atrophy and, in some cases, other ciliopathy phenotypes. However, the molecular mechanism leading to disease is currently unknown. Here, we used Kif3a−/−;Kif3b−/− (knockout) 3T3 cells, which cannot make cilia, to characterize these mutations. While reexpression of KIF3B(E250Q) and KIF3B(L523P) did not rescue ciliogenesis, reexpression of wildtype or KIF3B(A334T) restored ciliogenesis to wildtype levels. Fluorescent tagging revealed that the E250Q mutant decorated microtubules and thus is a rigor mutation. The L523P mutation, in the alpha-helical stalk domain, surprisingly did not affect formation of the KIF3A/KIF3B/KAP3 complex but instead impaired motility along microtubules. Lastly, expression of the A334T motor was reduced in comparison to all other motors, and this motor displayed an impaired ability to disperse the Golgi complex when artificially linked to this high-load cargo. In summary, this work uses cell-based assays to elucidate the molecular effects of disease-causing mutations in the KIF3B subunit on the kinesin-2 holoenzyme.
A delayed and unsynchronized ovary development as revealed by transcriptome of brain and pituitary of Coilia nasus
Authors: Yu Ziyan , Gao Zongshuai , Zeng Yun , Li Mingyou , Xu Gangchun , Ren Mingchun , Zhu Yunxia , Liu Dong
Published: April 2024

Coilia nasus is an anadromous fish that has been successfully domesticated in the last decade due to its high economic value. The fish exhibits a delayed ovary development during the reproductive season, despite breeding and selection for five to six offspring. The molecular mechanism of the delayed ovary development is still unknown, so the obstacles have not been removed in the large-scale breeding program. This study aims to investigate the key genes regulating ovarian development by comparing the transcriptomes of ovarian-stage IV and stage II brain/pituitary of Coilia nasus. Ovarian stages were validated by histological sections. A total of 75,097,641 and 66,735,592 high-quality reads were obtained from brain and pituitary transcriptomes, respectively, and alternatively spliced transcripts associated with gonadal development were detected. Compared to ovarian Ⅱ- brain, 515 differentially expressed genes (DEGs) were upregulated and 535 DEGs were downregulated in ovarian Ⅳ- brain, whereas 470 DEGs were upregulated and 483 DEGs were downregulated in ovarian Ⅳ- pituitary compared to ovarian Ⅱ- pituitary. DEGs involved in hormone synthesis and secretion and in the GnRH signaling pathway were screened. Weighted gene co-expression network analysis identified gene co-expression modules that were positively correlated with ovarian phenotypic traits. The hub genes Smad4 and TRPC4 in the modules were co-expressed with DEGs including Kiss1 receptor and JUNB, suggesting that ovarian development is controlled by a hypothalamic-pituitary-gonadal axis. Our results have provided new insights that advance our understanding of the molecular mechanism of C. nasus reproductive functions and will be useful for future breeding.
Improving the stability and transdermal permeability of phycocyanin loaded cubosomes
Authors: Zhu Chune , Duan Wenjuan , Jing Hui , Long Jieyu , Huang Ying , Huang Di , Wu Chuanbin
Published: April 2024

Instability and low transdermal permeability of protein antioxidants are major obstacles to resist oxidative stress in transdermal drug delivery system. To overcome these shortcomings, cubosomes were developed as an advanced transdermal delivery system to improve stability and transdermal absorption of the model antioxidant phycocyanin in this study. Glyceryl monooleate and poloxamer 407 (P407) were used to prepare cubosomes as carrier matrix and stabilizer, respectively. Phycocyanin loaded cubosomes (PC-cubosomes) were prepared by the emulsification and homogenization method. A 33 full factorial design was used to optimize the cubosome formulations. The final optimal PC-cubosomes possessed an average particle size of 183.2 ± 0.5 nm and a negative surface charge as well as achieved a high encapsulation efficiency of 87.2% ± 2.7%. PC-cubosomes appeared as nano-sized and well-shaped spheres with highly ordered cubical structures. The residual amount of phycocyanin in PC-cubosomes was 3-fold higher than that in the free drug solution after 10 days ultraviolet radiation exposure. In vitro release kinetics of phycocyanin from PC-cubosomes fitted to the Higuchi kinetic model, indicating that phycocyanin released from cubosomes mainly attributed to drug diffusion and dissolution. PC-cubosomes also exhibited higher permeability (39.79 μg⋅cm−2⋅hour−1) across the rat skin than phycocyanin solution (16.33 μg⋅cm−2⋅hour−1). Furthermore, PC-cubosomes were easily taken up by keratinocytes, thereby achieving a prolonged anti-oxidative stress effect. These results therefore suggested that cubosomes could be a promising transdermal delivery system to improve the stability and transdermal permeability of phycocyanin.
GABAergic interneuron diversity and organization are crucial for the generation of human-specific functional neural networks in cerebral organoids
Authors: Heesen Sebastian H. , Köhr Georg
Published: April 2024

This mini review investigates the importance of GABAergic interneurons for the network function of human-induced pluripotent stem cells (hiPSC)-derived brain organoids. The presented evidence suggests that the abundance, diversity and three-dimensional cortical organization of GABAergic interneurons are the primary elements responsible for the creation of synchronous neuronal firing patterns. Without intricate inhibition, coupled oscillatory patterns cannot reach a sufficient complexity to transfer spatiotemporal information constituting physiological network function. Furthermore, human-specific brain network function seems to be mediated by a more complex and interconnected inhibitory structure that remains developmentally flexible for a longer period when compared to rodents. This suggests that several characteristics of human brain networks cannot be captured by rodent models, emphasizing the need for model systems like organoids that adequately mimic physiological human brain function in vitro.
Application of imaging modalities for endovascular thrombectomy of large core infarcts in clinical practice
Authors: Zeng Hai , Zhu Qingfeng
Published: April 2024

Four randomized controlled trials of large infarct core volume (LICV) included three imaging modalities: non-contrast CT (NCCT)-Alberta Stroke Program Early CT Score (ASPECTS), diffusion-weighted imaging (DWI)-ASPECTS, and NCCT-ASPECTS combined with CTP (CT perfusion). However, there is no clear consensus on the optimal imaging modality for endovascular thrombectomy (EVT) trials of large core infarcts. The variety and complexity of imaging modalities make it difficult to apply them in clinical practice. By familiarizing ourselves with these imaging modalities, we can better apply them in the clinic and correctly screen patients with large core infarcts in the anterior circulation who can benefit from EVT therapy.
Global trends of acupuncture clinical research on analgesia from 2010 to 2023: a bibliometric and visualization analysis
Authors: Li Zhi-Qiang , Wang Xue-Feng , Feng Cao , Fei Yu-Tong , Liu Jian-Ping
Published: April 2024

Objective

Acupuncture, acknowledged as a potent non-pharmacological therapy, is frequently employed to alleviate pain. Despite its widespread use, there has been a lack of overarching bibliometric analysis of clinical research on acupuncture analgesia. We aimed to summarize current patterns, hotspots, and development trends in this field through bibliometric analysis.

Methods

This study evaluates academic publications retrieved from the Web of Science database (2010.01–2023.09) concerning acupuncture analgesia in clinical settings. All primary and secondary studies on humans were included. To track global developmental trends, we employed several software for analyzing annual publication volumes, countries/regions, institutions, authors, cited authors, journals, cited journals, references, and keywords and to draw collaborative networks and reference co-citation network maps.

Results

The final search encompassed 7,190 relevant studies, including 1,263 randomized controlled trials (RCTs) and 1,293 systematic reviews and meta-analyses. The results indicated a gradual increase in the number of annual publications on acupuncture analgesia in clinical practice. Among countries and institutions, China (2,139) and Chengdu University of Traditional Chinese Medicine (258) ranked first. Liang FR (89 articles) was the most prolific author, while MacPherson H (604) was the most cited author. MEDICINE (455) was the most productive journal, and Pain (2,473/0.20) ranked first in both the frequency and centrality of cited journals. Notably, the most frequently cited reference was a systematic review of individual patient data on acupuncture carried out for chronic pain that was published by Vickers Andrew J in 2012 (156). Burst analysis identified frontier research areas for 2010–2020, encompassing network meta-analysis, case reports, dry needling, lumbar disc herniation, cancer, post-herpetic neuralgia, insomnia, and bibliometric analysis.

Conclusion

This study outlines current trends and potential future research hotspots in clinical acupuncture analgesia over the past decade. Findings emphasize the necessity for enhanced international collaboration to improve research output and translation.
Research on active and passive schemes for safety improvement of nuclear energy hydrogen production system
Authors: Gao Qunxiang , Sun Qi , Zhang Ping , Zhao Gang , Peng Wei
Published: April 2024

Nuclear hydrogen production has the advantages of large-scale and low carbon emissions, and is expected to play an active role in the energy transition process. However, the storage and transportation of hydrogen pose potential risks of leakage and diffusion when connected to high-pressure hydrogen storage tanks and pipelines. To address this concern, this study focused on designing three distinct safety improvement schemes tailored for potential hydrogen leakage accidents. These schemes encompassed a passively distributed arrangement of obstacles (Scheme 1), a passively centralized arrangement of obstacles (Scheme 2), and an active fan array blowing (Scheme 3). Numerical simulation methods were applied on extensive spatial scales for relevant calculations. The results revealed that all three schemes effectively reduced the diffusion distance of combustible hydrogen. Specifically, at lower ambient wind speeds, Scheme 1, Scheme 2, and Scheme 3 achieved the shortest diffusion distances of 123 m, 56 m, and 46 m, respectively. Meanwhile, at higher ambient wind speeds, the corresponding distances were 282 m, 100 m, and 79 m. These results collectively offer valuable insights to mitigate the risk of leakage accidents in nuclear hydrogen production systems.
Establishment of a prognostic model for gastric cancer patients who underwent radical gastrectomy using machine learning: a two-center study
Authors: Lu Tong , Lu Miao , Liu Haonan , Song Daqing , Wang Zhengzheng , Guo Yahui , Fang Yu , Chen Qi , Li Tao
Published: April 2024

Objective

Gastric cancer is a prevalent gastrointestinal malignancy worldwide. In this study, a prognostic model was developed for gastric cancer patients who underwent radical gastrectomy using machine learning, employing advanced computational techniques to investigate postoperative mortality risk factors in such patients.

Methods

Data of 295 patients with gastric cancer who underwent radical gastrectomy at the Department of General Surgery of Affiliated Hospital of Xuzhou Medical University (Xuzhou, China) between March 2016 and November 2019 were retrospectively analyzed as the training group. Additionally, 109 patients who underwent radical gastrectomy at the Department of General Surgery Affiliated to Jining First People’s Hospital (Jining, China) were included for external validation. Four machine learning models, including logistic regression (LR), decision tree (DT), random forest (RF), and gradient boosting machine (GBM), were utilized. Model performance was assessed by comparing the area under the curve (AUC) for each model. An LR-based nomogram model was constructed to assess patients’ clinical prognosis.

Results

Lasso regression identified eight associated factors: age, sex, maximum tumor diameter, nerve or vascular invasion, TNM stage, gastrectomy type, lymphocyte count, and carcinoembryonic antigen (CEA) level. The performance of these models was evaluated using the AUC. In the training group, the AUC values were 0.795, 0.759, 0.873, and 0.853 for LR, DT, RF, and GBM, respectively. In the validation group, the AUC values were 0.734, 0.708, 0.746, and 0.707 for LR, DT, RF, and GBM, respectively. The nomogram model, constructed based on LR, demonstrated excellent clinical prognostic evaluation capabilities.

Conclusion

Machine learning algorithms are robust performance assessment tools for evaluating the prognosis of gastric cancer patients who have undergone radical gastrectomy. The LR-based nomogram model can aid clinicians in making more reliable clinical decisions.
Plasma miRNA expression profile in pediatric pineal pure germinomas
Authors: Fakhry Mona , Elayadi Moatasem , Elzayat Mariam G. , Samir Omar , Maher Eslam , Taha Hala , El-Beltagy Mohamed , Refaat Amal , Zamzam Manal , Abdelbaki Mohamed S. , Sayed Ahmed A. , Kieran Mark , Elhaddad Alaa
Published: April 2024

Background

Pure germinomas account for 40% of pineal tumors and are characterized by the lack of appreciable tumor markers, thus requiring a tumor biopsy for diagnosis. MicroRNAs (miRNA) have emerged as potential non-invasive biomarkers for germ cell tumors and may facilitate the non-invasive diagnosis of pure pineal germinomas.

Material and methods

A retrospective chart review was performed on all patients treated at the Children’s Cancer Hospital Egypt diagnosed with a pineal region tumor between June 2013 and March 2021 for whom a research blood sample was available. Plasma samples were profiled for miRNA expression, and DESeq2 was used to compare between pure germinoma and other tumor types. Differentially expressed miRNAs were identified. The area under the curve of the receive;r operating characteristic curve was constructed to evaluate diagnostic performance.

Results

Samples from 39 pediatric patients were available consisting of 12 pure germinomas and 27 pineal region tumors of other pathologies, including pineal origin tumors [n = 17; pineoblastoma (n = 13) and pineal parenchymal tumors of intermediate differentiation (n = 4)] and others [n = 10; low-grade glioma (n = 6) and atypical teratoid rhabdoid tumor (n = 4)]. Using an adjusted p-value <0.05, three miRNAs showed differential expression (miR-143-3p, miR-320c, miR-320d; adjusted p = 0.0058, p = 0.0478, and p = 0.0366, respectively) and good discriminatory power between the two groups (AUC 90.7%, p < 0.001) with a sensitivity of 25% and a specificity of 100%.

Conclusion

Our results suggest that a three-plasma miRNA signature has the potential to non-invasively identify pineal body pure germinomas which may allow selected patients to avoid the potential surgical complications.
Case report: Concurrent intrathecal and intravenous pembrolizumab for metastatic melanoma with leptomeningeal disease
Authors: Dan Xiang , Huang Mengxi , Sun Zhaochen , Chu Xiaoyuan , Shi Xin , Chen Yitian
Published: April 2024

Leptomeningeal disease (LMD) is a serious cancer complication associated with poor prognosis. Approximately 5%–25% of patients with melanoma develop LMD. Currently, no standard treatment protocol exists and very few cases have been reported. Despite ongoing advances in new therapies, treatment options for LMD remain limited. Herein, we report a case of intrathecal pembrolizumab administration in a patient with melanoma and LMD. Intrathecal pembrolizumab administration was feasible and safe at the doses tested. Drawing from this case, along with our expertise and the existing evidence on systemic immunotherapy, we propose that an immunotherapy approach involving intrathecal administration for patients with LMD from melanoma warrants additional exploration in clinical trials.
Unusual coexistence: branchial cleft cyst harboring papillary thyroid carcinoma with lymph node metastasis — a rare case report and clinical insights
Authors: Wang Wei-Tao , Ni Xi-Hao , Gu Yong-Xue , An Ran , Wang Chang-Liang , Zhang Jun
Published: April 2024

Background

The simultaneous occurrence of Branchial Cleft Cyst (BCC) and Papillary Thyroid Carcinoma (PTC) represents an unusual malignant tumor, with cases featuring associated lymph node metastasis being particularly rare. This combination underscores an increased potential for metastasis, and the assessment of neck masses, particularly on the lateral aspect, may inadvertently overlook the scrutiny of the thyroid. Therefore, healthcare providers should exercise vigilance, especially in patients over the age of 40, regarding the potential for neck masses to signify metastasis from thyroid malignancies. Currently, surgical intervention stands as the primary effective curative method, while the postoperative administration of radioactive iodine therapy remains a topic of ongoing debate.

Case report

In the presented case, a 48-year-old male patient with a right neck mass underwent surgical intervention. The procedure included the excision of the right neck mass, unilateral thyroidectomy with isthmus resection, and functional neck lymph node dissection under tracheal intubation and general anesthesia. Postoperative pathology findings revealed the coexistence of a BCC with metastatic PTC in the right neck mass, as well as papillary carcinoma in the right thyroid lobe. Lymph node metastasis was observed in the central and levels III of the right neck.

Conclusion

The rare amalgamation of a BCC with PTC and concurrent lymph node metastasis underscores the invasive nature of this malignancy. Healthcare professionals should be well-acquainted with its clinical presentation, pathological characteristics, and diagnostic criteria. A multidisciplinary approach is strongly recommended to enhance patient outcomes.
Successful treatment with fludarabine and cyclophosphamide in a VEXAS syndrome patient with associated myelodysplastic syndrome: a case report and systematic review
Authors: Bellman Polina , Gonzalez-Lugo Jesus D. , Shahzad Moazzam , Amin Muhammad Kashif , Khalid Muhammad Fareed , Suleman Nahid , Ahmed Nausheen , Singh Anurag K. , Yacoub Abdulraheem , Zhang Da , McGuirk Joseph P. , Mushtaq Muhammad Umair
Published: April 2024

Vacuoles, E1 syndrome, X-linked, autoinflammatory, somatic (VEXAS) syndrome is a chronic inflammatory disorder that affects various organ systems. It is associated with hematologic malignancies and is generally refractory to therapies. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) may be considered for selected patients. We report a case wherein systemic and hematological manifestations completely resolved in a patient with VEXAS and associated myelodysplastic syndrome (MDS), following the administration of fludarabine and cyclophosphamide as part of the preparation for allo-HSCT. We conducted a systematic literature review and included 86 patients with VEXAS syndrome and associated MDS. Most cases presented with musculoskeletal involvement (71%) and anemia (72%) with lower-risk MDS. Most patients responded to corticosteroids (CS) but had a recurrence of symptoms with CS taper and were refractory to other immunosuppressive agents. Hypomethylating agents and Janus kinase inhibitors achieved a complete response in some cases. Further research is needed to develop more effective treatment strategies.
Content validity of the electronic faces thermometer scale for pain in children: is a picture worth more than a thousand words?
Authors: Höök Angelica , Castor Charlotte , Björk Maria , Forsgren Emma , Muszta Anders , Nilsson Stefan
Published: April 2024

Introduction

Early recognition of pain in children is crucial, and their self-report is the primary source of information. However, communication about pain in healthcare settings can be challenging. For non-verbal communication regarding different symptoms, children prefer digital tools. The electronic Faces Thermometer Scale (eFTS) utilizes a universal design with colors, face emojis, and numbers on an 11-point scale (0–10) for pain assessment. The aim of this study was to establish content validity of the eFTS for pain assessments in children.

Methods

A mixed methods design was used. The study took place at a university hospital in eastern Sweden, involving 102 children aged 8–17 years who visited outpatient clinics. Participants were presented with 17 pictures representing varying pain levels and asked to assess hypothetical pain using the eFTS. A think-aloud approach was employed, prompting children to verbalize their thoughts about assessments and the eFTS. Quantitative data were analyzed using descriptive and comparative statistics, together with a qualitative approach for analysis of think-aloud conversations.

Results

A total of 1,734 assessments of hypothetical pain using the eFTS were conducted. The eFTS differentiated between no pain (level 0–1) and pain (level 2–10). However, no clear agreement was found in the differentiation between hypothetical pain intensity levels (level 2–10). The analysis revealed that children utilized the entire scale, ranging from no pain to high pain, incorporating numbers, colors, and face emojis in their assessments.

Discussion

The variability in assessments was influenced by prior experiences, which had an impact on the statistical outcome in our study. However, employing the think-aloud method enhances our understanding of how children utilize the scale and perceive its design, including the incorporation of emotion-laden anchors. Children express a preference for using the eFTS to assess their pain during hospital visits.
Cumulative sum learning curve analysis of tubularized incised plate repair for hypospadias: a study of a single surgeon with a single surgical procedure
Authors: Li Jiaqiang , Zhang Jiaqian , Diao Hongwang , He Zhuoyuan , Li Shoulin , Yin Jianchun
Published: April 2024

Purpose

To ascertain the quantity of instances by which a single surgeon achieves competency and proficiency in using tubularized incised plate (TIP) technique for the repair of distal and mid-shaft hypospadias using the cumulative sum (CUSUM) analysis.

Methods

We retrospectively evaluated patients with distal and mid-shaft hypospadias who were treated by a single surgeon between 2015 and 2021, using a single primary TIP technique with a de-epithelialized Byars flap. Data including type of hypospadias, age at surgery, curvature, operation time (OT), length of the reconstructed urethra, and postoperative outcomes were collected and assessed. CUSUM was used to assess the trends in OT and complication rate (CR) in order to generate the learning curve. The evolution of OT and CR can be divided into three phases: learning, competence, and proficiency.

Results

CUSUM identified three phases in the learning curves of all TIP repairs. The median OT decreased from 135 min [interquartile range (IQR) = 125–155] to 92 min (IQR = 80–100) (P < 0.001), CR decreased from 28 (28%) to 8 (5.3%) (P < 0.001), and reoperations decreased from 15 (15.2%) to 4 (2.6%) (P < 0.001). According to the CUSUM learning curve, technical competency plateaued after the 99th case, and both OT and CR entered a significantly declining proficiency phase after the 231st case. Further, when the neourethral length exceeded the total average, total complications, urethrocutaneous fistula, and reoperations increased (P = 0.013, P = 0.006, and P = 0.028, respectively).

Conclusions

Our study suggests that surgeons performing TIP repair may reach technical competency and achieve proficiency after operating on 99,231 cases, respectively. Moreover, the longer the neourethral length, the higher is the CR.