bluuebunny/crossref_metadata_2025 huggingface dataset:
Knowledge and Perception of and Attitude toward a Premarital Screening Program in Qatar: A Cross-Sectional Study
Authors: Al-Shafai Mashael , Al-Romaihi Aisha , Al-Hajri Noora , Islam Nazmul , Adawi Karam
Published: April 2022

Premarital screening (PMS) is a primary preventive measure to decrease the incidence of certain genetic disorders and sexually transmitted diseases. This study aimed to explore the knowledge and perception of and the attitude toward PMS and predictors of knowledge and attitude. A cross-sectional study was conducted among Qatar University students using an online survey. Multivariable regression analyses were used to identify factors associated with PMS knowledge and attitude. A total of 476 students participated in the study; 424 (89.1%) were females; two-thirds were 18–21 years old. Only 100 participants had heard about PMS. Knowledge of PMS was significantly associated with females, students enrolled in a health-related college, and non-consanguineous marriage of a participant’s parents. The majority of the participants agreed that genetic diseases are psychological and economic burdens. For attitude, only 178 participants were willing to cancel marriages, given incompatible PMS results. The following factors were positively associated with attitude: PMS knowledge, enrollment in a health-related college, and the belief that PMS does not interfere with destiny. Our study findings revealed that despite the mandatory PMS in Qatar, the study participants, future couples, had low knowledge about the program. Therefore, strategies to increase awareness of PMS should be considered toward improving its outcomes.
The Emerging Threat of Plasmodium knowlesi Malaria Infection: A Concept Paper on the Vulnerable Factors in Human
Authors: Naserrudin Nurul Athirah , Hod Rozita , Jeffree Mohammad Saffree , Ahmed Kamruddin , Hassan Mohd Rohaizat
Published: April 2022

In South-East Asia (SEA), there has been an increase in the trend of detected and reported cases of Plasmodium knowlesi malaria in the last few decades. This higher transmission rate within SEA countries is attributed to the distribution of the Macaque, banded leaf monkeys, and Anopheles mosquito in this region. This study aims to propose a concept that highlights the integration of vulnerability factors to P. knowlesi malaria infection. The relevant literature on the vulnerability factors of P. knowlesi was reviewed. Any theories and models that could be integrated to support the factors were also explored throughout this study. Exposure to P. knowlesi malaria was found to be influenced by sociodemographic, socioeconomic, environmental, social context, belief, and human behaviour factors. However, these factors were commonly discussed separately in existing studies. For better disease prevention and control, all these factors should be explored collectively, to facilitate research aimed at generating a deeper understanding of the vulnerability factors to P. knowlesi malaria from various perspectives, including the genetic, sociodemographic, socioeconomic, environmental, sociocultural beliefs, and human behaviour of the population.
Virtual 3D City Models
Authors: Stouffs Rudi
Published: April 2022

Virtual 3D city models, in varying forms of extent and detail, are becoming more common, yet their usage might still be limited [...]
Comparison between Nanoparticle Encapsulation and Surface Loading for Lysosomal Enzyme Replacement Therapy
Authors: Muntimadugu Eameema , Silva-Abreu Marcelle , Vives Guillem , Loeck Maximilian , Pham Vy , del Moral Maria , Solomon Melani , Muro Silvia
Published: April 2022

Poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) enhance the delivery of therapeutic enzymes for replacement therapy of lysosomal storage disorders. Previous studies examined NPs encapsulating or coated with enzymes, but these formulations have never been compared. We examined this using hyaluronidase (HAse), deficient in mucopolysaccharidosis IX, and acid sphingomyelinase (ASM), deficient in types A–B Niemann–Pick disease. Initial screening of size, PDI, ζ potential, and loading resulted in the selection of the Lactel II co-polymer vs. Lactel I or Resomer, and Pluronic F68 surfactant vs. PVA or DMAB. Enzyme input and addition of carrier protein were evaluated, rendering NPs having, e.g., 181 nm diameter, 0.15 PDI, −36 mV ζ potential, and 538 HAse molecules encapsulated per NP. Similar NPs were coated with enzyme, which reduced loading (e.g., 292 HAse molecules/NP). NPs were coated with targeting antibodies (> 122 molecules/NP), lyophilized for storage without alterations, and acceptably stable at physiological conditions. NPs were internalized, trafficked to lysosomes, released active enzyme at lysosomal conditions, and targeted both peripheral organs and the brain after i.v. administration in mice. While both formulations enhanced enzyme delivery compared to free enzyme, encapsulating NPs surpassed coated counterparts (18.4- vs. 4.3-fold enhancement in cells and 6.2- vs. 3-fold enhancement in brains), providing guidance for future applications.
Emerging Roles of Motile Epidermal Chloroplasts in Plant Immunity
Authors: Irieda Hiroki
Published: April 2022

Plant epidermis contains atypical small chloroplasts. However, the physiological role of this organelle is unclear compared to that of large mesophyll chloroplasts, the well-known function of which is photosynthesis. Although knowledge of the involvement of chloroplasts in the plant immunity has been expanded to date, the differences between the epidermal and mesophyll chloroplasts are beyond the scope of this study. Given the role of the plant epidermis as a barrier to environmental stresses, including pathogen attacks, and the immune-related function of chloroplasts, plant defense research on epidermal chloroplasts is an emerging field. Recent studies have revealed the dynamic movements of epidermal chloroplasts in response to fungal and oomycete pathogens. Furthermore, epidermal chloroplast-associated proteins and cellular events that are tightly linked to epidermal resistance against pathogens have been reported. In this review, I have focused on the recent progress in epidermal chloroplast-mediated plant immunity.
Identification of Entry Inhibitors against Delta and Omicron Variants of SARS-CoV-2
Authors: Lee Richard Kuan-Lin , Li Tian-Neng , Chang Sui-Yuan , Chao Tai-Ling , Kuo Chun-Hsien , Pan Max Yu-Chen , Chiou Yu-Ting , Liao Kuan-Ju , Yang Yi , Wu Yi-Hsuan , Huang Chen-Hao , Juan Hsueh-Fen , Hsieh Hsing-Pang , Wang Lily Hui-Ching
Published: April 2022

Entry inhibitors against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgently needed to control the outbreak of coronavirus disease 2019 (COVID-19). This study developed a robust and straightforward assay that detected the molecular interaction between the receptor-binding domain (RBD) of viral spike protein and the angiotensin-converting enzyme 2 (ACE2) receptor in just 10 min. A drug library of 1068 approved compounds was used to screen for SARS-CoV2 entry inhibition, and 9 active drugs were identified as specific pseudovirus entry inhibitors. A plaque reduction neutralization test using authentic SARS-CoV-2 virus in Vero E6 cells confirmed that 2 of these drugs (Etravirine and Dolutegravir) significantly inhibited the infection of SARS-CoV-2. With molecular docking, we showed that both Etravirine and Dolutegravir are preferentially bound to primary ACE2-interacting residues on the RBD domain, implying that these two drug blocks may prohibit the viral attachment of SARS-CoV-2. We compared the neutralizing activities of these entry inhibitors against different pseudoviruses carrying spike proteins from alpha, beta, gamma, and delta variants. Both Etravirine and Dolutegravir showed similar neutralizing activities against different variants, with EC50 values between 4.5 to 5.8 nM for Etravirine and 10.2 to 22.9 nM for Dolutegravir. These data implied that Etravirine and Dolutegravir may serve as general spike inhibitors against dominant viral variants of SARS-CoV-2.
IGFBP-3 Regulates Mitochondrial Hyperfusion and Metabolic Activity in Ocular Surface Epithelia during Hyperosmolar Stress
Authors: Stuard Whitney L. , Guner Melis K. , Robertson Danielle M.
Published: April 2022

In the eye, hyperosmolarity of the precorneal tear film triggers inflammation and the development of dry eye disease (DED), a highly prevalent condition that causes depression and disability in severe forms. A member of the insulin-like growth factor (IGF) family, the IGF binding protein-3 (IGFBP-3), is a pleiotropic protein with known roles in growth downregulation and survival. IGFBP-3 exerts these effects by blocking IGF-1 activation of the type 1 IGF-receptor (IGF-1R). Here, we examined a new IGF-independent role for IGFBP-3 in the regulation of mitochondrial and metabolic activity in ocular surface epithelial cells subject to hyperosmolar stress and in a mouse model of DED. We found that hyperosmolar stress decreased IGFBP-3 expression in vitro and in vivo. Treatment with exogenous IGFBP-3 induced an early, transient shift in IGF-1R to mitochondria, followed by IGFBP-3 nuclear accumulation. IGFBP-3 nuclear accumulation increased protein translation, blocked the hyperosmolar-mediated decrease in oxidative phosphorylation through the induction of mitochondrial hyperfusion, and restored corneal health in vivo. These data indicate that IGFBP-3 acts a stress response protein in ocular surface epithelia subject to hyperosmolar stress. These findings may lead to the development of first-in-class therapeutics to treat eye diseases with underlying mitochondrial dysfunction.
Released ATP Mediates Spermatozoa Chemotaxis Promoted by Uterus-Derived Factor (UDF) in Ascaris suum
Authors: Wang Qiushi , He Ruijun , Zhang Qi , Shan Jin , Zhao Yanmei , Wang Xia
Published: April 2022

Fertilization requires sperm migration toward oocytes and subsequent fusion. Sperm chemotaxis, a process in which motile sperm are attracted by factors released from oocytes or associated structures, plays a key role in sperm migration to oocytes. Here, we studied sperm chemotaxis in the nematode Ascaris suum. Our data show that uterus-derived factor (UDF), the protein fraction of uterine extracts, can attract spermatozoa. UDF is heat resistant, but its activity is attenuated by certain proteinases. UDF binds to the surface of spermatozoa but not spermatids, and this process is mediated by membranous organelles that fuse with the plasma membrane. UDF induces spermatozoa to release ATP from intracellular storage sites to the extracellular milieu, and extracellular ATP modulates sperm chemotaxis. Moreover, UDF increases protein serine phosphorylation (pS) levels in sperm, which facilitates sperm chemotaxis. Taken together, we revealed that both extracellular ATP and intracellular pS signaling are involved in Ascaris sperm chemotaxis. Our data provide insights into the mechanism of sperm chemotaxis in Ascaris suum.
Aflatoxin B1 Metabolism of Reared Alphitobius diaperinus in Different Life-Stages
Authors: Meijer Nathan , Nijssen Rosalie , Bosch Marlou , Boers Ed , van der Fels-Klerx H. J.
Published: April 2022

The presence of carcinogenic aflatoxins in food and feed is a major issue. In prior studies, aflatoxin B1 (AfB1) and known primary metabolites were absent from Lesser Mealworm (LMW, Alphitobius diaperinus) reared on contaminated diets. LMW is a promising alternative protein source. The objectives of this stu\dy were to determine whether LMW can be reared on AfB1-contaminated feed in each life-stage, and to gather more insight into potential metabolites formed. Results suggested no adverse effects in terms of survival/growth when three stages of LMW (larvae, pre-pupae, beetles) were exposed to feed containing AfB1 concentrations of 200 and 600 µg/kg for 48 h. Insect and frass samples were analyzed by LC-MS/MS and high-resolution MS to, respectively, quantify concentrations of AfB1 and its major metabolites, and determine secondary metabolites. No AfB1 or major metabolites were quantified in the insect samples. Mass balance calculations showed that up to 40% of spiked AfB1 could be recovered in the frass, in the form of AfB1, aflatoxicol and AfM1. HRMS results suggested the presence of additional metabolites in the frass, but, due to lack of commercially available reference standards for these compounds, exact identification and quantification was not possible. More research is needed to verify the absence of toxicity.
Hypertension and Arrhythmias: A Clinical Overview of the Pathophysiology-Driven Management of Cardiac Arrhythmias in Hypertensive Patients
Authors: Marazzato Jacopo , Blasi Federico , Golino Michele , Verdecchia Paolo , Angeli Fabio , De Ponti Roberto
Published: April 2022

Because of demographic aging, the prevalence of arterial hypertension (HTN) and cardiac arrhythmias, namely atrial fibrillation (AF), is progressively increasing. Not only are these clinical entities strongly connected, but, acting with a synergistic effect, their association may cause a worse clinical outcome in patients already at risk of ischemic and/or haemorrhagic stroke and, consequently, disability and death. Despite the well-known association between HTN and AF, several pathogenetic mechanisms underlying the higher risk of AF in hypertensive patients are still incompletely known. Although several trials reported the overall clinical benefit of renin–angiotensin–aldosterone inhibitors in reducing incident AF in HTN, the role of this class of drugs is greatly reduced when AF diagnosis is already established, thus hinting at the urgent need for primary prevention measures to reduce AF occurrence in these patients. Through a thorough review of the available literature in the field, we investigated the basic mechanisms through which HTN is believed to promote AF, summarising the evidence supporting a pathophysiology-driven approach to prevent this arrhythmia in hypertensive patients, including those suffering from primary aldosteronism, a non-negligible and under-recognised cause of secondary HTN. Finally, in the hazy scenario of AF screening in hypertensive patients, we reviewed which patients should be screened, by which modality, and who should be offered oral anticoagulation for stroke prevention.
Assessment of Neuromuscular and Psychological Function in People with Recurrent Neck Pain during a Period of Remission: Cross-Sectional and Longitudinal Analyses
Authors: Alalawi Ahmed , Devecchi Valter , Gallina Alessio , Luque-Suarez Alejandro , Falla Deborah
Published: April 2022

The aim of this study was to examine for the presence of differences in neuromuscular and psychological function in individuals with recurrent neck pain (RNP) or chronic neck pain (CNP) following a whiplash trauma compared to healthy controls. A secondary aim was to examine whether neuromuscular characteristics together with psychological features in people with RNP were predictive of future painful episodes. Multiple features were assessed including neck disability, kinesiophobia, quality of life, cervical kinematics, proprioception, activity of superficial neck flexor muscles, maximum neck flexion and extension strength, and perceived exertion during submaximal contractions. Overall, those with RNP (n = 22) and CNP (n = 8) presented with higher neck disability, greater kinesiophobia, lower quality of life, slower and irregular neck movements, and less neck strength compared to controls (n = 15). Prediction analysis in the RNP group revealed that a higher number of previous pain episodes within the last 12 months along with lower neck flexion strength were predictors of higher neck disability at a 6-month follow-up. This preliminary study shows that participants with RNP presented with some degree of altered neuromuscular features and poorer psychological function with respect to healthy controls and these features were similar to those with CNP. Neck flexor weakness was predictive of future neck disability.
Is the JAK-STAT Signaling Pathway Involved in the Pathogenesis of Depression?
Authors: Gałecka Małgorzata , Szemraj Janusz , Su Kuan-Pin , Halaris Angelos , Maes Michael , Skiba Aleksandra , Gałecki Piotr , Bliźniewska-Kowalska Katarzyna
Published: April 2022

(1) Background: Only 60–70% of depressed patients respond to standard antidepressant treatments. Hence, it is essential to search for new, effective and safe therapies for unmet clinical needs of treatment-resistant depression (TRD). Agents targeting the components of the JAK-STAT signaling pathway have been shown to be relevant in immunology and are commonly used in the treatment of many hematological, rheumatological and dermatological diseases. The aim of this study was to investigate the role of elements of the JAK-STAT signaling pathway in the etiopathogenesis of depressive disorders. (2) Methods: A total of 290 subjects took part in the study (190 depressed patients, 100 healthy controls). Sociodemographic data were collected. The severity of depressive symptoms was assessed using the Hamilton Depression Rating Scale (HDRS). The gene expression at the mRNA protein levels of JAK (JAK1-JAK3) and STAT (STAT1-STAT5) was assessed by using RT-PCR and ELISA. (3) Results: Increased expression of JAK3 and decreased expression of STAT1 were observed in the group of depressed patients. (4) Conclusions: Further studies are necessary to determine whether moderation of the JAK-STAT signaling pathways is involved in the treatment of depression.
Effects of Obstructive Sleep Apnea on Epilepsy, and Continuous Positive Airway Pressure as a Treatment Option
Authors: Jo Hyunjin , Choi Sujung , Kim Dongyeop , Joo Eunyeon
Published: April 2022

Purpose: To compare the characteristics of obstructive sleep apnea (OSA) between patients with epilepsy and patients without epilepsy and to investigate CPAP (Continuous Positive Airway Pressure) effect on seizures. Methods: Medical and polysomnography (PSG) data from 235 adult OSA patients with epilepsy (OE; 183 males; mean age, 49.8 years) and 268 age- and sex-matched OSA patients without epilepsy (OSE; 216 males; mean age, 51.3 years), obtained between March 2014 and May 2020 and housed in a database in a university-affiliated hospital, were retrospectively reviewed. All subjects completed surveys addressing comorbidities and medications, and sleep-related questionnaires including the Insomnia Severity Index, Epworth Sleepiness Scale, Pittsburgh Sleep Quality Index, and Beck Depression Inventory-II. Results: Compared with the OSE group, the OE group reported fewer sleep-related complaints and less severe OSA-related PSG parameters, with a lower apnea-hypopnea index (24.9 vs. 33.4 events/h; p < 0.003), arousal index (23.3 vs. 30.8 events/h; p < 0.001), and oxygen desaturation index (19.6 vs. 28.8; p < 0.002). The OE group had fewer smokers and lower alcohol consumption but a higher body mass index (27.0 vs. 25.9 kg/m2; p < 0.001). No correlations were observed between OSA-related PSG parameters and epilepsy-related factors, such as age at seizure onset, seizure type, frequency of seizures, presence of nocturnal seizures, and number of antiseizure medications, in the OE group. Patients with OE who demonstrated good compliance with CPAP therapy exhibited a decrease in seizure frequency. Conclusions: The OE group exhibited less severe disease characteristics than their age- and sex-matched OSE counterparts. Nevertheless, because the coexistence of OSA and epilepsy is high, CPAP therapy can reduce the frequency of seizures. Therefore, it is important to evaluate the presence of OSA in patients with epilepsy and to treat the conditions concurrently.
Solar-Light-Driven Ag9(SiO4)2NO3 for Efficient Photocatalytic Bactericidal Performance
Authors: Taki Malaa M. , Mahdi Rahman I. , Al-Keisy Amar , Alsultan Mohammed , Al-Bahnam Nabil Janan , Majid Wan Haliza Abd. , Swiegers Gerhard F.
Published: April 2022

Photocatalytic materials are being investigated as effective bactericides due to their superior ability to inactivate a broad range of dangerous microbes. In this study, the following two types of bacteria were employed for bactericidal purposes: Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). The shape, crystal structure, element percentage, and optical properties of Ag9(SiO4)2NO3 were examined after it was successfully synthesized by a standard mixing and grinding processing route. Bactericidal efficiency was recorded at 100% by the following two types of light sources: solar and simulated light, with initial photocatalyst concentration of 2 µg/mL, and 97% and 95% of bactericidal activity in ultra-low photocatalyst concentration of 0.2 µg/mL by solar and simulated light, respectively, after 10 min. The survival rate was studied for 6 min, resulting in 99.8% inhibition at the photocatalyst dose of 2 µg/mL. The mechanism of bactericidal efficiency was found to be that the photocatalyst has high oxidation potential in the valence band. Consequently, holes play a significant part in bactericidal efficiency.
Irradiation Effects in Polymer Composites for Their Conversion into Hybrids
Authors: Zaharescu Traian , Mariş Marius
Published: April 2022

In this paper several aspects of profound modifications caused by high energy exposures are presented as possible candidates for the efficient adjusting processing of polymer materials. The class of hybrid composites receives special attention due to the large spectrum of formulations, where the interphase interaction decisively influences the material properties. They represent potential start points for the intimate uniformity of hybrid morphologies. Their radiation processing turns composites onto hybrid morphology with expected features, because the transferred energy is spent for the modification of components and for their compatibility. The essential changes achieved in radiation processed composites explain the new material behavior and durability based on the peculiar restructuring of polymer molecules that occurred in the polymer phase. During high energy irradiation, the interaction between intermediates born in the constitutive phases may convert the primary composites into hybrids, integrating them into large applicability spheres. During the radiation exposure, the resulting hybrids gain a continuous dispersion by means of new chemical bonds. This type of compounds achieves some specific structural modifications in the polymer phase, becoming stable hybrid composites. The functional properties of hybrids definitely influence the material behavior due to the molecular changes based on the structural reasons. The radiolysis of the vulnerable component becomes an appropriate opportunity for the creation of new material with improved stability. The radiation treatment is a proper conversion procedure by which common mixtures may become continuously reorganized. This review presents several examples for the radiation modifications induced by radiation exposure that allow the compatibilization and binding of components as well as the creation of new structures with improved properties. This approach provides the reference patterns for the extension of radiation processing over the well-conducted adjustments of polymer composites, when certain material features are compulsorily required. From this review, several solutions for the adjustment of regular polymer composites into hybrid systems may become conceivable by the extended radiation processing.
Novel Hypertrophic Cardiomyopathy Diagnosis Index Using Deep Features and Local Directional Pattern Techniques
Authors: Gudigar Anjan , Raghavendra U. , Samanth Jyothi , Dharmik Chinmay , Gangavarapu Mokshagna Rohit , Nayak Krishnananda , Ciaccio Edward J. , Tan Ru-San , Molinari Filippo , Acharya U. Rajendra
Published: April 2022

Hypertrophic cardiomyopathy (HCM) is a genetic disorder that exhibits a wide spectrum of clinical presentations, including sudden death. Early diagnosis and intervention may avert the latter. Left ventricular hypertrophy on heart imaging is an important diagnostic criterion for HCM, and the most common imaging modality is heart ultrasound (US). The US is operator-dependent, and its interpretation is subject to human error and variability. We proposed an automated computer-aided diagnostic tool to discriminate HCM from healthy subjects on US images. We used a local directional pattern and the ResNet-50 pretrained network to classify heart US images acquired from 62 known HCM patients and 101 healthy subjects. Deep features were ranked using Student’s t-test, and the most significant feature (SigFea) was identified. An integrated index derived from the simulation was defined as 100·log10(SigFea/2)  in each subject, and a diagnostic threshold value was empirically calculated as the mean of the minimum and maximum integrated indices among HCM and healthy subjects, respectively. An integrated index above a threshold of 0.5 separated HCM from healthy subjects with 100% accuracy in our test dataset.
Multi-Decadal Seawall-Induced Topo-Bathymetric Perturbations along a Highly Energetic Coast
Authors: Lerma Alexandre Nicolae , Billy Julie , Bulteau Thomas , Mallet Cyril
Published: April 2022

Seawalls are commonly used worldwide to protect urbanized sea fronts. These alongshore protection structures are often blamed for hydro-sedimentary dynamics perturbations, but without clear and generalizable conclusions on long-term morphodynamic effects. In this paper, evolutions of beaches are studied from 1966 to 2021, comparing the urbanized sea front of Lacanau seaside resort (Aquitaine France) and adjacent natural areas. A large-scale spatiotemporal multisource dataset is used to derivate several indicators and evaluate the characteristics and magnitude of passive and active erosion related to a large riprap seawall at a highly energetic meso–macro tidal coast. The most dramatic manifestation of the presence of the seawall (passive erosion) is the beach lowering and the reduction of beach variability at the seasonal and interannual timescale in front of the seawall. However, recent evolutions are roughly similar at the seawall-backed beach than at the natural sector, indicating no specific active seawall influence on beach erosion or recovery. The perturbations directly attributable to the seawall (active erosion) are limited to temporary end-effect, slight perturbation of outer bar pattern and the setup of a slight platform around the depth of closure. The adverse effects are currently manageable, but they require a new strategy in view of the chronic shoreline retreat at adjacent sectors and the expected effects of climate change.
Integrated Berth and Crane Scheduling Problem Considering Crane Coverage in Multi-Terminal Tidal Ports under Uncertainty
Authors: Jiang Meixian , Zhou Jian , Feng Jiajia , Zhou Lin , Ma Fangzheng , Wu Guanghua
Published: April 2022

In this work, we study the integrated berth and crane scheduling problem in a tidal port with multiple terminals, considering the uncertainties, tides, maximum coverage of cranes and interference between cranes. For coping with the uncertainties, a certain number of randomly generated samples are used to evaluate the solutions, and slack variables are introduced to reduce the impact caused by the variation in vessel arrival and crane operational efficiency. A novel nonlinear mixed integer programming model is first formulated for the problem to minimize the sum of expectation and variance of costs under all samples. An improved adaptive genetic algorithm, combining a simulated annealing mechanism and greedy construction strategy, is developed and implemented by MATLAB. The feasibility and validity of the algorithm and the benefits of multi-terminal collaborative scheduling strategy under uncertainty are evaluated through numerical experiments. The results show that the algorithm can obtain feasible scheduling solutions with higher quality. Compared to the strategy that considers either the uncertainty or the multi-terminal collaborative mechanism, the resulting solution considering both can effectively reduce the cost and improve the competitiveness of the port.
Prediction of the Deterioration of FRP Composite Properties Induced by Marine Environments
Authors: Vizentin Goran , Vukelic Goran
Published: April 2022

In this paper, a model for the prediction of fatigue life degradation of fiber-reinforced (FRP) composite materials exposed for prolonged periods to real marine environments is proposed. The data collected during the previous phases of a more comprehensive research of real marine environment-induced changes of mechanical properties in FRP composites are used to assess the influence of these changes on the durability characteristics of composites. Attention is paid to the classification societies’ design and exploitation rules on this matter. The need for the modification of the process used for obtaining composite material S–N curves, considering the influence of the marine environment, is studied. A regression analysis of the experimental data is conducted, resulting in a mathematical model of strength degradation over time. The regression analysis shows an acceptable correlation value. The S–N curves for E-glass/polyester composites with three different fiber layout configurations are evaluated and modified to encompass the findings of this research.
Insight into the Taxonomic Resolution of the Pleosporalean Species Associated with Dead Woody Litter in Natural Forests from Yunnan, China
Authors: Wanasinghe Dhanushka N. , Ren Guang-Cong , Xu Jian-Chu , Cheewangkoon Ratchadawan , Mortimer Peter E.
Published: April 2022

In the course of investigating the systematics of woody litter micromycete associates in Yunnan Province, China, we found one new species in Phaeoseptaceae, one new genus and three new species in Sulcatisporaceae from 16 specimens collected (ten collections of ascomycetous teleomorphs, four collections of hyphomycetous and two collections of coelomycetes anamorphs) from Ailaoshan, Chuxiong, Diqing, Honghe, Kunming, Lancang, Mengla and Yuxi in Yunnan Province. These taxonomic novelties were recognized with the aid of morphological comparisons and phylogenetic analyses of multiple gene sequences (non-translated loci and protein-coding regions). Pleopunctum menglaense sp. nov. is accommodated in Phaeoseptaceae (Pleosporales) based on its hyphomycetous anamorph, which is characterized by superficial sporodochia on the host surface, macronematous, mononematous, cylindrical, unbranched, aseptate, hyaline and smooth-walled conidiophores, monoblastic, terminal, hyaline conidiogenous cells, hyaline, muriform α conidia, and brown, muriform β conidia with tri-lobed wing like basal cells. Kazuakitanaka gen. nov. (type: K. yuxiensis) is introduced in Sulcatisporaceae (Massarineae, Pleosporales) for a saprobic ascomycete with teleomorphic and anamorphic (coelomycetous) features. The teleomorph possesses globose to subglobose ascomata with acentric ostiole, a peridial wall of textura angularis to textura prismatica, cylindric-clavate, pedicellate asci with an ocular chamber, and 1–2-septate, hyaline, fusiform, guttulate ascospores with a distinct mucilaginous sheath. The anamorph features pycnidial conidiomata, phialidic, ampulliform to cylindrical, hyaline conidiogenous cells and ampulliform to cylindrical, one-to-three-septate, hyaline, guttulate conidia. Loculosulcatispora was known only from its anamorph of L. thailandica. We observed the teleomorph of Loculosulcatispora hongheensis sp. nov. and amended the generic description of Loculosulcatispora accordingly. Loculosulcatispora hongheensis is characterized by globose to subglobose ascomata with a central ostiole, a peridial wall of textura angularis to globosa, branched, septate, pseudoparaphyses, clavate asci with a short pedicel and a minute ocular chamber and hyaline, fusiform, 1-septate ascospores with a thick irregular mucilaginous sheath. This study provides some insights into the diversity of fungi on dead woody litter in terrestrial habitats.
Xenocentrism and Formal Education: Evaluating Its Impact on the Behavior of Chilean Consumers
Authors: Camacho Luis J. , Ramírez-Correa Patricio , Salazar-Concha Cristian
Published: April 2022

Understanding social behavior and explaining its implications is essential when examining consumer xenocentric behavior. This study evaluated the direct effects of consumer xenoncentrism on product attitude and purchase intention of imported products and analyzed the moderating impact of formal education on xenocentric consumer behavior considering groups with higher and low formal education levels. Structural equation modeling technique and multigroup analysis based on samples collected from Chilean consumers were used to analyze the research model. There were 295 effective online questionnaires (42.4% females and 57.6% males). This study’s findings suggest that consumer xenocentric effects are directly related to imported products’ product attitude (G: R2 = 0.254; L: R2 = 0.121; H: R2 = 0.327) and purchase intention (G: R2 = 0.454; L: R2 = 0.469; H: R2 = 0.365). In addition, findings exhibit that xenocentric consumer behaviors are more significant when associated with formal educational level (G: 0.575; L: 0.640; H: 0.443). Therefore, as education levels increase, the xenocentric effect also increases. An important application of these findings is that education in emerging countries and developing economies should strengthen local production valorization and promote marketing strategies that foster the sustainable consumption of products manufactured in their own countries.
Understanding Residents’ Perceptions of the Ecosystem to Improve Park–People Relationships in Wuyishan National Park, China
Authors: He Siyuan , Su Yang
Published: April 2022

A healthy park–people relationship depends essentially on the fair and sustainable maintenance of rural livelihood. When a protected area is designated, rural people may face restrictions on access to land and resource use. In Wuyishan of China, we analyzed the role of traditional tea cultivation during consistent protected area management to find ways to maintain the stability of this social-ecological system in the new national park era. Based on the social-ecological system meaning perception, we used an intensive social survey to investigate residents’ perception of the ecosystem in terms of tea cultivation and its interaction with conservation policies. Results showed that tea cultivation brought major household income and was associated with multiple cultural services. Protected area management affected land use, and conservation outcomes were more obvious to farmers than economic and social ones. We argue that the multi-functionality of the forest-tea system has the potential to benefit both the local people and the public through conservation-compatible activities at three levels: to regulate biophysical elements in the land plot, to link production and market at the mountain level, and to secure tenure and encourage community participation at the landscape level. This knowledge co-production approach revealed that to avoid a negative park–people relationship, traditional knowledge and people’s right to benefit must be respected.
Using Photovisualizations to Gain Perspectives on River Conservation over Time
Authors: Braconnier Meryl , Morse Cheryl E. , Hurley Stephanie
Published: April 2022

The Missisquoi River originates in a densely forested, hilly, and lightly populated region in northern Vermont, USA, flowing north until it crosses the Canadian border. The upper American stretches of the river are federally designated as “Wild and Scenic” in recognition of its outstanding natural, cultural, and recreational values. This paper reports on the place-based and river-focused perspectives of rural residents who live and work along the Missisquoi River and its tributaries and who are the recipients of Vermont’s shifting river management strategies. The mixed methods research drew on participant observation, interviews, and interpretations of photovisualizations (PVZs). The PVZ method identified the different geographical imaginaries held by residents and conservation professionals, demonstrating that PVZs can be used as a method to foster dialogue about sense of place and conservation initiatives. Visual aids can help unveil the complex, temporal relationships between landowners and the adjacent waterways, which in turn influence participation in river restoration efforts.
A Quantitative Approach to Microvariation: Negative Marking in Central Romance
Authors: Pescarini Diego
Published: April 2022

This work presents an exploratory data analysis of the syntactic distribution of pre- and postverbal negation (N1 and N2) in a corpus of data gathered from two linguistic atlases, the Linguistic Atlas of France (ALF) and the Italo-Swiss Atlas (AIS). Metadata concerning the distribution of N1 and N2 across dialects and syntactic contexts are analyzed with the r package Rbrul. Multiple logistic regression allows us to assess how independent variables affect the presence/absence of N1/N2. Geographical and grammatical factors are examined; the latter concern mainly clause typing and negative concord, i.e., the co-occurrence of clausal negation and a negative word. The data from the two atlases are first analyzed separately and eventually merged in order to strengthen the statistical significance. Both geographical and grammatical factors prove to be significant. In particular, the preliminary findings show that N1 is more likely retained in sentences containing another negative word, the incidence of N1 varies according to the type of co-occurring negative word, and veridicality has a mild effect on N2 but not N1.
Perception of Hidden Confidence in Neutral Expressions: Interactions of Facial Attractiveness, Self-Esteem, and Names to Be Addressed by
Authors: Azami Ritsuko , Kikutani Mariko , Kitamura Hideya
Published: April 2022

Even when a person is portraying a neutral expression, their internal feelings can be reflected subtly on their face, and observers can perceive them. A previous study took facial photographs of female models while wearing attractive and unattractive clothing. Although the models displayed neutral expressions for both cases, their faces while wearing attractive clothing were perceived as more attractive because, it was argued, the attractive clothing raised their confidence, which was observable on the neutral faces. The present study aimed to replicate this. Envisaging being addressed by a specific name (given name, nickname, and formal title) are used to alter the models’ internal states instead of clothing. Twenty-one Japanese models took three photographs of their faces while imagining (1) being addressed by names they like, (2) being addressed by names they dislike, and (3) being addressed by their surnames with titles. A number of Japanese observers viewed three images of the same model at once and ordered them according to their attractiveness (Study 1) and confidence (Study 2). The images in condition (1) were perceived as more attractive/confident than other images. This suggests that being addressed by the name we like can raise our confidence momentarily, and it reflects subtly in neutral expressions.
Less Direct, More Analytical: Eye-Movement Measures of L2 Idiom Reading
Authors: Senaldi Marco S. G. , Titone Debra A.
Published: April 2022

Idioms (e.g., break the ice, spill the beans) are ubiquitous multiword units that are often semantically non-compositional. Psycholinguistic data suggests that L1 readers process idioms in a hybrid fashion, with early comprehension facilitated by direct retrieval, and later comprehension inhibited by factors promoting compositional parsing (e.g., semantic decomposability). In two eye-tracking experiments, we investigated the role of direct retrieval and compositional analysis when idioms are read naturally in sentences in an L2. Thus, French–English bilingual adults with French as their L1 were tested using English sentences. For idioms in canonical form, Experiment 1 showed that prospective verb-related decomposability and retrospective noun-related decomposability guided L2 readers towards bottom-up figurative meaning access over different time courses. Direct retrieval played a lesser role, and was mediated by the availability of a congruent “cognate” idiom in the readers’ L1. Next, Experiment 2 included idioms where direct retrieval was disrupted by a phrase-final language switch into French (e.g., break the glace, spill the fèves). Switched idioms were read comparably to switched literal phrases at early stages, but were penalized at later stages. These results collectively suggest that L2 idiom processing is mostly compositional, with direct retrieval playing a lesser role in figurative meaning comprehension.
Cotton and Flax Textiles Leachables Impact Differently Cutaneous Staphylococcus aureus and Staphylococcus epidermidis Biofilm Formation and Cytotoxicity
Authors: Catovic Chloé , Abbes Imen , Barreau Magalie , Sauvage Catherine , Follet Jacques , Duclairoir-Poc Cécile , Groboillot Anne , Leblanc Sandra , Svinareff Pascal , Chevalier Sylvie , Feuilloley Marc G. J.
Published: April 2022

Bacteria can bind on clothes, but the impacts of textiles leachables on cutaneous bacteria remain unknown. Here, we studied for the first time the effects of cotton and flax obtained through classical and soft ecological agriculture on the representatives S. aureus and S. epidermidis bacteria of the cutaneous microbiota. Crude flax showed an inhibitory potential on S. epidermidis bacterial lawns whereas cotton had no effect. Textile fiber leachables were produced in bacterial culture media, and these extracts were tested on S. aureus and S. epidermidis. Bacterial growth was not impacted, but investigation by the crystal violet technique and confocal microscopy showed that all extracts affected biofilm formation by the two staphylococci species. An influence of cotton and flax culture conditions was clearly observed. Flax extracts had strong inhibitory impacts and induced the formation of mushroom-like defense structures by S. aureus. Conversely, production of biosurfactant by bacteria and their surface properties were not modified. Resistance to antibiotics also remained unchanged. All textile extracts, and particularly soft organic flax, showed strong inhibitory effects on S. aureus and S. epidermidis cytotoxicity on HaCaT keratinocytes. Analysis of flax leachables showed the presence of benzyl alcohol that could partly explain the effects of flax extracts.
Weight Loss and Fat Metabolism during Multi-Day High-Altitude Sojourns: A Hypothesis Based on Adipocyte Signaling
Authors: Pramsohler Stephan , Burtscher Martin , Rausch Linda , Netzer Nikolaus C.
Published: April 2022

Several publications and random observations have reported weight loss in high-altitude sojourners of both sexes. This could be a result of multiple adaptations, which hypoxia and mountaineering provoke on a cellular and organic level. Several publications have discussed the effect on appetite-regulating hormones to be one of the main contributing factors. We aimed to review the available data and show the current state of knowledge regarding nutritional aspects in high altitude with a special focus on fatty dietary forms. To reach this aim we conducted a literature search via PubMed according to the PRISMA 2020 protocol to identify relevant studies. We found that very few studies cover this field with scientifically satisfying evidence. For final analysis, reviews as well as papers that were not clearly related to the topic were excluded. Six articles were included discussing hormonal influences and the impact of exercise on appetite regulation as well as genetic factors altering metabolic processes at altitude. Leptin expression seems to be the biggest contributor to appetite reduction at altitude with an initial increase followed by a decrease in the course of time at high altitude. Its expression is greatly dependent on the amount of white adipose tissue. Since the expression of leptin is associated with an increased β-oxidation of fatty acids, a high-fat diet could be advantageous at a certain time point in the course of high-altitude sojourns.
Determinants of Antibody Response to a Third SARS-CoV-2 mRNA Vaccine Dose in Solid Organ Transplant Recipients: Results from the Prospective Cohort Study COVAC-Tx
Authors: Balsby Daniel , Nilsson Anna Christine , Möller Sören , Lindvig Susan Olaf , Davidsen Jesper Rømhild , Abazi Rozeta , Poulsen Mikael Kjær , Holden Inge Kristine , Justesen Ulrik Stenz , Bistrup Claus , Johansen Isik Somuncu
Published: April 2022

Background: We studied factors related to humoral response in solid organ transplant (SOT) recipients following a three-dose regimen of an mRNA-based SARS-CoV-2 vaccine. Method: This was a prospective study of SOT recipients who received a third homologous dose of the BNT162b2 (Pfizer–BioNTech) vaccine. The anti-spike S1 IgG response was measured using the SARS-CoV-2 IgG II Quant assay (Abbott Laboratories) with a cut-off of 7.1 BAU/mL. Multiple logistic regression was used to determine the factors associated with humoral response. Results: In total, 395 SOT recipients were included. Anti-spike IgG was detected in 195/395 (49.4%) patients after the second dose and 261/335 (77.9%) patients after the third dose. The overall mean increase in antibody concentration after the third dose was 831.0 BAU/mL (95% confidence interval (CI) 687.4–974.5) and 159 (47.5%) participants had at least a 10-fold increase in antibody concentration after the third dose. The increase in antibody concentration was significantly higher among patients with detectable antibodies after the second dose than those without. Cumulative time from transplantation and liver recipients was positively associated with an antibody response, whereas older age, administration of prednisolone, and proliferation inhibitors were associated with diminished antibody response. Conclusion: Although the third dose of the BNT162b2 vaccine improved humoral responses among SOT non-responders following the second dose, the overall response remained low, and 22.1% did not develop any response. Patients at risk of a diminished vaccine response require repeated booster doses and alternative treatment approaches.
An Integrated Approach for Deciphering Hydrogeochemical Processes during Seawater Intrusion in Coastal Aquifers
Authors: Abu Salem Hend S. , Gemail Khaled S. , Junakova Natalia , Ibrahim Amin , Nosair Ahmed M.
Published: April 2022

For managing the freshwater in the worldwide coastal aquifers, it is imperative to understand the hydrogeochemical processes and flow patterns in the mixing freshwater/saltwater zone. The Egyptian Nile Delta aquifer is a typical example. The management of seawater intrusion (SWI) requires detailed investigations of the intrusion wedge and the dynamic processes in the mixing zone. Thus, a multidisciplinary approach was applied based on holistic hydrogeochemical, statistical analysis, and DC resistivity measurements to investigate the lateral and vertical changes in groundwater characteristics undergoing salinization stressor. The results of cross plots and ionic deviations of major ions, hydrochemical facies evolution diagram (HFE-D), and seawater mixing index (SMI) were integrated with the resistivity results to show the status of the SWI where the intrusion phase predominates in ~2/3 of the study are (~70 km radius) and the compositional thresholds of Na, Mg, Cl, and SO4 are 600, 145, 1200, and 600 mg/L, respectively, indicating that the wells with higher concentrations than these thresholds are affected by SWI. Moreover, the results demonstrate the efficiency of combining hydrogeochemical facies from heatmap and resistivity investigations to provide a large-scale characterization of natural and anthropogenic activities controlling aquifer salinization to support decision-makers for the long-term management of coastal groundwater.