bluuebunny/crossref_metadata_2025 huggingface dataset:
Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids
Authors: de Lemos Luisa , Antas Pedro , Ferreira Inês S. , Santos Inês Paz , Felgueiras Beatriz , Gomes Catarina M. , Brito Catarina , Seabra Miguel C. , Tenreiro Sandra
Published: March 2024

Purpose

Diabetic retinopathy (DR) is a complication of diabetes and a primary cause of visual impairment amongst working-age individuals. DR is a degenerative condition in which hyperglycaemia results in morphological and functional changes in certain retinal cells. Existing treatments mainly address the advanced stages of the disease, which involve vascular defects or neovascularization. However, it is now known that retinal neurodegeneration and inflammation precede these vascular changes as early events of DR. Therefore, there is a pressing need to develop a reliable human in vitro model that mimics the early stage of DR to identify new therapeutic approaches to prevent and delay its progression.

Methods

Here, we used human-induced pluripotent stem cells (hiPSCs) differentiated into three-dimensional (3D) retinal organoids, which resemble the complexity of the retinal tissue. Retinal organoids were subjected to high-glucose conditions to generate a model of early DR.

Results

Our model showed well-established molecular and cellular features of early DR, such as (i) loss of retinal ganglion and amacrine cells; (ii) glial reactivity and inflammation, with increased expression of the vascular endothelial-derived growth factor (VEGF) and interleukin-1β (IL-1β), and monocyte chemoattractant protein-1 (MCP-1) secretion; and (iii) increased levels of reactive oxygen species accompanied by activation of key enzymes involved in antioxidative stress response.

Conclusion

The data provided highlight the utility of retinal organoid technology in modelling early-stage DR. This offers new avenues for the development of targeted therapeutic interventions on neurodegeneration and inflammation in the initial phase of DR, potentially slowing the disease’s progression.
Perioperative taurine or taurolidine supplementation on clinical outcomes: A systematic review with meta-analysis
Authors: Ho Kwok M. , Harahsheh Yusra
Published: March 2024

Taurine is a conditionally essential amino acid that has been reported to have antioxidative and immunomodulatory effects either directly or through its interactions with gut microbiome. Although taurine has been widely used as a health supplement and also for treatment of congestive heart failure in Japan, its roles in the perioperative setting have not been well characterized. The purpose of this systematic review and meta-analysis was to assess whether oral or intravenous taurine, or its derivatives such as taurolidine, can offer benefits compared to placebo in the perioperative setting. Non-randomized studies, trials assessed topical taurine or taurolidine, or trials did not report clinical outcomes were excluded. Six randomized-controlled-trials (RCTs) involving a total of 596 adult surgical patients, in PubMed, MEDLINE and EMBASE databases on September 14, 2023 were identified and meta-analyzed using a fixed-effect model. Biases were assessed by reporting individual trial elements. Taurine or taurolidine (which is readily metabolized by hydrolysis to taurine) supplementation was associated with a reduction in all-cause infection (5 trials: pooled odds ratio (OR) 0.59; 95% confidence interval (CI) 0.38–0.92; p = 0.020) but not delirium (2 trials: OR 0.63, 95% CI 0.38–1.04; p = 0.071) or mortality (4 trials: OR 0.82, 95% CI 0.43–1.58; p = 0.557) compared to placebo. Using the ‘trim and fill’ technique to adjust for publication bias did not change the favorable effect of taurine on risk of infection (OR 0.61, 95% CI 0.40–0.95). Because the number of studies included was small, the promising benefits of taurine on risks of perioperative infection should be confirmed by adequately-powered RCTs.
Progress, application and challenges of liver organoids
Authors: Sang Chen , Lin Jian , Ji Shuyi , Gao Qiang
Published: March 2024

The liver, the largest solid organ in the body, is susceptible to metabolic diseases and malignant tumors. Studying its physiological and pathological processes helps to optimize the clinical treatment. Organoids are a novel tool for studying physical development, disease mechanisms, and high-throughput drug screening due to their similarity in composition, structure, and function to internal organs. Recent studies have shown that stem cells, hepatocytes, or cholangiocytes can form “liver organoids” under the synergistic action of specific extracellular matrix and various signaling molecules. This review outlines techniques for generating liver organoids that maximally recapitulate the liver structure and functions in vitro and thoroughly discusses the customary applications of organoids derived from liver tissue, induced pluripotent stem cells (iPSCs) and liver tumors. In this review, a meticulous analysis is provided of the comparatively advanced culture systems used in the construction of liver cancer-derived organoids. Additionally, we reviewed the progress of liver organoids in disease modeling, drug efficacy, and toxicity evaluation, in hopes of generating innovative ideas for the research and applications of liver organoids.
Counter-Stereotypes and Attitudes Toward Gender and LGBTQ Equality
Authors: Jung Jae-Hee , Tavits Margit
Published: March 2024

Insights from social psychology and the gender and politics literature, as well as discussions and campaigns in the policymaking world, suggest that exposure to counter-stereotypes about gender roles might improve people's attitudes toward gender equality and LGBTQ rights. The authors test this expectation by conducting five survey experiments (N=6,916) and a separate, follow-up experiment (N=3,600) in the US context using counter-stereotypical treatments commonly encountered in the real world. They examine both political and non-political attitudes, manipulate stereotypes about both men and women, and provide visual as well as textual stimuli. The treatments undermined stereotypes about the gender roles depicted in the counter-stereotypical exemplars. However, they failed to alter respondents' generic core beliefs about women and men and increase equitable attitudes. The results improve our understanding of how stereotypes contribute to gender and anti-LGBTQ bias.
A Practical Introduction to Regression Discontinuity Designs
Authors: Cattaneo Matias D. , Idrobo Nicolas , Titiunik Rocío
Published: March 2024

In this Element, which continues our discussion in Foundations, the authors provide an accessible and practical guide for the analysis and interpretation of Regression Discontinuity (RD) designs that encourages the use of a common set of practices and facilitates the accumulation of RD-based empirical evidence. The focus is on extensions to the canonical sharp RD setup that we discussed in Foundations. The discussion covers (i) the local randomization framework for RD analysis, (ii) the fuzzy RD design where compliance with treatment is imperfect, (iii) RD designs with discrete scores, and (iv) and multi-dimensional RD designs.
Interrelationships Among the Rice Export Prices of Major Exporting Countries: In Search of the Leader and Followers
Authors: Mottaleb Khondoker Abdul , Durand-Morat Alvaro
Published: March 2024

By examining the econometric interrelationships of rice export prices of India, Thailand, Vietnam, Pakistan, the U.S., Argentina, Brazil, and Uruguay, this study examines the most influential rice-exporting countries in the international rice export market. The sampled countries are the source of more than 80% of the global rice exports. In the process, this study relies on online data portals of the United Nations and country-level sources for aromatic and non-aromatic rice export price information from India, Pakistan, and Thailand. This study employed the Prais-Winsten and Autoregressive Distributive Lag (ARDL) estimation processes with the error correction procedure. Combining the findings from both models, this study concludes that in terms of interactions, the non-aromatic long-grain rice price of the U.S., and the aromatic rice price of Pakistan are the most correlated prices in the international rice market. Considering the number of countries influenced by the price of a given country, our results indicate that Vietnam is the price leader in the non-aromatic long-grain market, followed by Argentina, Brazil, and India. India, the largest exporter, is the fourth top price leader. In the case of aromatic rice, Thailand is the absolute leader. Many countries in Asia and Africa rely on rice imports to meet domestic demand, where the food security situation is already precarious. Based on the findings, this study suggests closely monitoring rice production and export trends of Vietnam, Argentina, Brazil, India and Thailand to provide early warning when necessary to better manage supply shocks in the international rice market.
Mack’s estimator motivated by large exposure asymptotics in a compound poisson setting
Authors: Engler Nils , Lindskog Filip
Published: March 2024

The distribution-free chain ladder of Mack justified the use of the chain ladder predictor and enabled Mack to derive an estimator of conditional mean squared error of prediction for the chain ladder predictor. Classical insurance loss models, that is of compound Poisson type, are not consistent with Mack’s distribution-free chain ladder. However, for a sequence of compound Poisson loss models indexed by exposure (e.g., number of contracts), we show that the chain ladder predictor and Mack’s estimator of conditional mean squared error of prediction can be derived by considering large exposure asymptotics. Hence, quantifying chain ladder prediction uncertainty can be done with Mack’s estimator without relying on the validity of the model assumptions of the distribution-free chain ladder.
Racial Discourses in Aegean Prehistory c. 1900: The Case of the Cupbearer Fresco at Knossos
Authors: Duray Anne
Published: March 2024

This article traces characterizations of the Cupbearer fresco, named after the large vessel the figure holds and uncovered at the site of Knossos in 1900, in light of the research agendas about the ‘races’ of the prehistoric Aegean and traditions of racial science current in late Victorian Britain. The head of the Cupbearer was compared to Classical Greek art, modern Cretan populations, and cranial remains from prehistoric contexts. Drawing from academic publications, articles in the press, and reports of the British Association for the Advancement of Science, the author situates the discourse surrounding the Cupbearer in the context of scholars seeking the origins of ‘European’ civilization in prehistory, and the creation of racial typologies, especially using cranial measurements and photography. The Cupbearer gained a dual status as a racial portrait comparable to past and present human populations, but also as a work of art that prefigured the later achievements of Classical Greece.
The origins and development of Article 16 of the Charter of fundamental rights
Authors: Hogan Hilary
Published: March 2024

When the Charter of Fundamental Rights was published in 2000, it was the first time that an international human rights instrument had recognised the freedom to conduct a business. To understand how conducting a business came to occupy the status of a fundamental right, I undertake an examination of the drafting process of Article 16 in Section 2. Early drafts protected the freedom to pursue an occupation, before the freedom to conduct a business eventually became a freestanding entitlement. This was thanks to the influence of Convention members associated with the European People’s Party who were instrumental in ensuring Article 16 was added to the draft Charter. In Section 3, I critically assess the claim contained in the Explanations to the Charter that Article 16 was simply a codification of pre-existing entitlements deriving from the case law of the Court of Justice. In Section 4, I examine the impact of Article 16 Many of the limitations that constrain the scope of Article 16 have been weakened, or have not proved to be the limitations that were originally envisaged. The Court of Justice’s defence of select areas, such as consumer protection, has shielded the deregulatory potential of Article 16 from view. Yet Article 16 has had a real and damaging impact on workers’ rights across the Union. Protecting the freedom to conduct a business in Article 16 means that a remarkably broad range of actions taken in the course of running a business are now accepted as the exercise of a fundamental right.
Revisiting the modelling framework for the unresolved scalar variance
Authors: Nikolaou Z. , Domingo P. , Vervisch L.
Published: March 2024

The unresolved scalar variance in large-eddy simulations of turbulent flows is a fundamental physical and modelling parameter. Despite its importance, relatively few algebraic models have been developed for this important variable with the most prominent models to date being the classic scale-similarity and gradient models. In this work a new generalized modelling framework based on reconstruction has been developed, which in contrast to classic modelling approaches allows the construction of base static variance models of arbitrary accuracy. It is demonstrated that higher-order reconstructions naturally lead to base static variance models of increased accuracy, and that the classic scale-similarity and gradient models are subsets of more general and higher-order models. The classic scale-similarity assumption for developing dynamic models is also revisited, and it is demonstrated that this can essentially be reinterpreted as a two-level reconstruction approach. Based on this result, a new general methodology is proposed that allows the construction of dynamic models for any given base static model, and a corresponding general reconstruction operator, algebraic or iterative. Consequently, improved static and dynamic models for the scalar variance are developed. The newly developed models are then thoroughly tested a priori using two high-fidelity direct numerical simulation databases corresponding to two substantially different flame and flow configurations, and are shown to outperform classic algebraic models for the variance.
WAND/SET THEORIES: A REALIZATION OF CONWAY’S MATHEMATICIANS’ LIBERATION MOVEMENT, WITH AN APPLICATION TO CHURCH’S SET THEORY WITH A UNIVERSAL SET
Authors: BUTTON TIM
Published: March 2024

Consider a variant of the usual story about the iterative conception of sets. As usual, at every stage, you find all the (bland) sets of objects which you found earlier. But you also find the result of tapping any earlier-found object with any magic wand (from a given stock of magic wands).

By varying the number and behaviour of the wands, we can flesh out this idea in many different ways. This paper's main Theorem is that any loosely constructive way of fleshing out this idea is synonymous with a ZF-like theory.

This Theorem has rich applications; it realizes John Conway's (1976) Mathematicians' Liberation Movement; and it connects with a lovely idea due to Alonzo Church (1974).
Navigating transformations: Climate change and international law
Authors: Mai Laura
Published: March 2024

The global climate crisis response envisioned by the Paris Agreement is commonly understood as demanding transformative change. International law, however, lacks a holistic conceptual framing for making sense of what such change would entail, how it might unfold, and who and what it will involve. Moreover, there has been little critical engagement with the question of what is at stake when invoking the notion of transformation. Contributing to the broader debate about what the climate crisis demands of international law, this article offers a critical conceptual appraisal of the notion of ‘transformation’. Conceptually, it describes transformative dynamics as processes which work towards radically different states of affairs that seem practically impossible under the status quo, but which could arguably be realized if different conditions were in place. Developing an ontology of transformative change, the article identifies heterogenous temporality, the actualization of impossible possibilities, and distributed engagement as three central features of transformations in climate crisis. Having laid the conceptual groundwork, the article then takes a critical turn and foregrounds unresolved tensions that run through transformation thinking. The aim here is to connect to critical discourses and show how these tensions can serve as entry points for international law to meaningfully engage with the notion of transformation. The article closes by offering some reflections on what engagement with the notion of transformation might mean for international law’s disciplinary identity, rationale, and sense of purpose.
Responding to a Non-Imminently Dying Patient’s Request for Pacemaker Deactivation
Authors: Gipe Kelsey
Published: March 2024

Based on Nathan Goldstein’s case report, “But I have a pacer…there is no point in engaging in hypothetical scenarios”: A Non-imminently Dying Patient’s Request for Pacemaker Deactivation, it is reasonable to conclude that it was, all-things-considered, ethically appropriate to grant the patient’s request to deactivate her pacemaker. Philosophically, and as a clinical bioethicist, I support the team’s decision to honor the patient’s request for pacemaker deactivation. However, it is worth exploring a bit further whether the distress on the part of the outside hospital’s ethics committee and providers—who declined to honor the patient’s request for pacemaker deactivation—may actually track something of moral significance. Might their discomfort around deactivation be “truth-tracking” in moral terms?
Oppressive Even As It Inspires
Authors: Visser-Maessen Laura , Van den Berk Jorrit
Published: March 2024

In this article, we trace the evolution of the connections between Black America and (Black) Europe since the mid-twentieth century and the study thereof. We do so through the lens of ‘Black American centrality,’ referring to the ways in which perceptions of Black America serve as an outsized reference point in European understandings of race, ‘Blackness,’ and Black (European) emancipation struggles. This allows for exploring the dilemmas that the, at times overwhelming, visibility of ‘Black America’ poses to Black Europeans, particularly during the current moment of flourishing Black European culture, politics, and scholarship. In that context, we show how both U.S.- and Europe-based scholars of Black American history and Black European history have approached Black American-European connections differently. The article concludes with suggestions for how these fields can engage with each other to develop academic approaches that account for but do not privilege the position of Black Americans within diasporic exchanges in the North Atlantic region, which is currently an underexplored area in diaspora studies.
Practice versus perception: A discussion of the humanitarian principle of independence in the context of migration
Authors: Hoagland Nicole , Arias Cubas Magdalena
Published: March 2024

The principle of independence is central to the International Red Cross and Red Crescent Movement's work with and for migrants. Independence requires humanitarian actors to retain their autonomy and resist any interference that might divert them from acting according to the principles of humanity, impartiality and neutrality. Yet, in the face of increasing securitization of migration and instrumentalization of aid and migrants, independence – in practice and perception – cannot be assumed. Drawing from current debates and primary research by the Red Cross Red Crescent Global Migration Lab, this article examines existing challenges in upholding independence in migration contexts and outlines recommendations for action.
Absence of High Frequency Echoes From Ionosondes During the 23–25 April 2023 Geomagnetic Storm; What Happened?
Authors: Habarulema John Bosco , Zhang Yongliang , Matamba Tshimangadzo , Buresova Dalia , Lu Gang , Katamzi‐Joseph Zama , Fagundes Paulo Roberto , Okoh Daniel , Seemala Gopi
Published: March 2024

We report an unusual event on absence of high frequency (HF) echoes in ionosonde observations from the ionospheric F2 region during the geomagnetic storm of 23–25 April 2023. This event was observed in both southern and northern hemispheres over two stations, Grahamstown (33.3°S, 26.5°E), South Africa and Pruhonice (50.0°N, 14.6°E), Czech Republic. Significant O/N2 depletion over the stations was observed by TIMED/GUVI, indicating a strong negative ionospheric storm. This is unique since absence of echoes in ionosonde measurements is usually due to strong radio absorption in the ionosphere associated with solar flares. However, there was no flare activity during the periods of “absent” F2 HF echoes. On the other hand, the ionosonde detected echoes from E‐layer. TIEGCM simulation reproduced TIMED/GUVI O/N2 depletion and showed that NmE was larger than NmF2 on dayside over Pruhonice. TIMED/GUVI O/N2 also showed a clear spatial gradient in the O/N2 depleted regions, suggesting F‐region ionosphere was tilted. By estimating the critical frequency of the F2 layer using GNSS observations, we have shown that it wasn't possible for the ionospheric electron density to reach depletion levels prohibiting reflection of HF echoes from ionosondes. We suggest that this phenomena may have been caused by either (a) maximum electron density of E layer exceeding that of F2 layer and/or (b) ionospheric tilting which made the signals to be reflected far away from the ionosonde locations.
Quantifying the Electron Energy of Mars Aurorae Through the Oxygen Emission Brightness Ratio at 130.4 and 135.6 nm
Authors: Soret Lauriane , Hubert Benoît , Gérard Jean‐Claude , Jain Sonal , Chirakkil K. , Lillis R. , Deighan J.
Published: March 2024

Mars discrete aurorae are caused by accelerated electrons precipitating into the atmosphere and interacting with species such as atomic oxygen. However, the energy of the electrons causing these aurorae remains currently unclear: no simultaneous and concurrent measurements of electron analyzers and spectrometers have been performed so far, preventing from assessing the exact energy of the downgoing auroral electrons. Several auroral emissions have been observed so far on Mars, among which are two oxygen emissions in the far ultraviolet at 130.4 and 135.6 nm. In this study, we simulate the vertical distribution of these auroral oxygen emissions with an electron transport calculation coupled with a radiative transfer model to account for the optical thickness of the atmosphere for the 130.4‐nm triplet. We show that the brightness ratio of these oxygen emissions is independent of the downward electron energy flux and only slightly depends on the atomic oxygen atmospheric composition. In contrast, the brightness ratio is strongly related to the initial energy of the auroral electrons. Measuring the brightness ratio is therefore a unique tool to remotely estimate the energy of the electrons causing the Mars discrete aurorae. We compare our model results with observations from the Emirates Mars Ultraviolet Spectrometer on board the Emirates Mars Mission and find that electrons with typical energies of 250–300 eV are compatible with the observed ratio of 5.
Stochastic in Space and Time: 1. Characterizing Orographic Gradients in Mean Runoff and Daily Runoff Variability
Authors: Forte A. M. , Rossi M. W.
Published: March 2024

Mountain topography alters the phase, amount, and spatial distribution of precipitation. Past efforts focused on how orographic precipitation can alter spatial patterns in mean runoff, with less emphasis on how time‐varying runoff statistics may also vary with topography. Given the importance of the magnitude and frequency of runoff events to fluvial erosion, we evaluated whether orographic patterns in mean runoff and daily runoff variability can be constrained using the global WaterGAP3 water model data. Model runoff data are validated against observational data in the contiguous United States, showing agreement with mean runoff in all settings and daily runoff variability in settings where rainfall‐runoff predominates. In snowmelt‐influenced settings, runoff variability is overestimated by the water model data. Cognizant of these limitations, we use the water model data to develop relationships between mean runoff and daily runoff variability and how these are mediated by snowmelt fraction in mountain topography globally. A global analysis of topographic controls on hydroclimatic variables using a random forest model was ambiguous. Instead, relationships between topography and runoff parameters are better assessed at the mountain range scale. Rulesets linking topography to mean runoff and snowmelt fraction are developed for three mid‐latitude mountain landscapes—British Columbia, European Alps, and Greater Caucasus. Increasing topographic elevation and relief together leads to higher mean runoff and lower runoff variability due to the increasing contribution of snowmelt. The three sets of empirical relationships developed here serve as the basis for a suite of numerical experiments in our companion manuscript (Part 2, Forte & Rossi, 2024a, https://doi.org.10.1002/2023JF007327).
Predicting the Electrical Conductivity of Partially Saturated Frozen Porous Media, a Fractal Model for Wide Ranges of Temperature and Salinity
Authors: Luo Haoliang , Jougnot Damien , Jost Anne , Teng Jidong , Mendieta Aida , Lin Gang , Thanh Luong Duy
Published: March 2024

The quantitative determination of liquid water content and salinity in soils is crucial for the preservation of hydrological environments and engineering infrastructures, especially in frozen regions. Electrical conductivity, as a fundamental physical parameter in electrical and electromagnetic non‐destructive techniques, varies significantly with the physical and chemical properties, such as pore water conductivity, salinity, water saturation, and temperature. In this study, accounting for pore size and tortuous length following fractal distributions, we develop a new capillary bundle model for variation of electrical conductivity as a function of temperature in broad water saturation and salinity ranges. In this new model, we consider the contributions of bulk and surface conductivities to the total electrical conductivity. To test this model, a series of laboratory experiments were carried out for different initial water saturations and salinities using an electrical resistance apparatus and a nuclear magnetic resonance method. The experimental results show that unfrozen water saturation and ionic concentration affect the electrical conductivity of unsaturated frozen soils. Furthermore, the proposed model is capable of fitting the main trends of the experimental data from the literature and acquired in this study in unfrozen‐frozen conditions for different water contents. Relying on the proposed model, we also determine the expression of the apparent formation factor, which is significantly sensitive to porosity, water saturation, and temperature. The predicted values of the apparent formation factor also agree very well with the experimental data. This new capillary bundle model provides a new perspective in interpreting electrical monitoring to easily deduce changes in key variables in the cryosphere such as liquid water content and moisture gradients.
Investigating the spatiotemporal characteristics and medical response during the initial COVID-19 epidemic in six Chinese cities
Authors: Lan Li , Li Gang , Mehmood Muhammad Sajid , Xu Tingting , Wang Wei , Nie Qifan
Published: March 2024

In the future, novel and highly pathogenic viruses may re-emerge, leading to a surge in healthcare demand. It is essential for urban epidemic control to investigate different cities’ spatiotemporal spread characteristics and medical carrying capacity during the early stages of COVID-19. This study employed textual analysis, mathematical statistics, and spatial analysis methods to examine the situation in six highly affected Chinese cities. The findings reveal that these cities experienced three phases during the initial outbreak of COVID-19: “unknown-origin incubation”, “Wuhan-related outbreak”, and “local exposure outbreak”. Cities with a high number of confirmed cases exhibited a multicore pattern, while those with fewer cases displayed a single-core pattern. The cores were distributed hierarchically in the central built-up areas of cities’ economic, political, or transportation centers. The radii of these cores shrank as the central built-up area’s level decreased, indicating a hierarchical decay and a core–edge structure. It suggests that decentralized built environments (non-clustered economies and populations) are less likely to facilitate large-scale epidemic clusters. Additionally, the deployment of designated hospitals in these cities was consistent with the spatial distribution of the epidemic; however, their carrying capacity requires urgent improvement. Ultimately, the essence of prevention and control is the governance of human activities and the efficient management of limited resources about individuals, places, and materials through leveraging IT and GIS technologies to address supply–demand contradictions.
An isochronous stress ratio logarithmic strain curve based clay creep model considering the effects of hardening and damage
Authors: Wang Peng , Tang Yin , Ren Peng , Zhang Hua
Published: March 2024

Creep is one of the typical mechanical properties of clay, and studying the creep mechanical properties of clay is of great significance to construction projects in clay sites. This study conducted creep tests on Chengdu clay and found that the soil mass underwent elastic deformation, decay creep deformation, steady-state creep deformation, and accelerated creep deformation. The isochronous stress ratio-logarithmic strain curves and their mathematical models were proposed to thoroughly analyze clay creep mechanical properties. Creep automatic feature points, such as linear elastic extreme point, initial yield point, long-term strength point, and plastic point, were identified on the curve. Considering the hardening and damage effects during creep loading, linear elastic and viscoelastic elements considering the time-dependent damage, a viscoplastic element considering the load hardening effect, and viscoplastic and plastic elements considering the load damage effect were established based on the element model and the Riemann–Liouville fractional derivative. Based on the mechanical properties of the whole clay creep process, the creep mechanical feature points, and the established element model, a clay creep model was proposed considering the hardening and damage effects. The rationality and regularity of the creep model were verified using the creep test data. This research accurately revealed the creep mechanical properties of clay and facilitated soil deformation prediction, thus providing technical guidance and references for construction projects in clay sites.
Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.
Authors: Ebadi Mostafa , Ahmadi Fatemeh , Tahmouresi Hossein , Pazhang Mohammad , Mollaei Saeed
Published: March 2024

Endophytic fungi are microorganisms that are considered as a potential source of natural compounds, and can be applied in various industries. The aims of this research were molecular identification of endophytic fungi isolated from the Gundelia tournefortii stems, and investigation their biological activities as well as phenolic and fatty acid profile. Surface sterilized stems of G. tournefortii were placed on potato dextrose agar (PDA) to isolate the fungal endophytes. Genomic DNA was extracted by CTAB method, and PCR amplification was performed by ITS 1 and ITS 4 as primers. The enzyme production of endophytic fungi was determined based on the formation of a clear zone that appeared around the colonies of fungus. The anti-oxidant activity was evaluated by measuring the amount of free radicals DPPH. Also, the total phenol and flavonoid contents were measured obtained by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Moreover, the separation and identification of phenolic acids and fatty acids were done by HPLC and GC, respectively. Phylogenetic analysis was done based on the Internal Transcribed Spacer (ITS) region, and five isolates were identified as following: Aspergillus niger, Penicillium glabrum, Alternaria alternata, A. tenuissima, and Mucor circinelloides. Evaluation of the enzymatic properties showed that P. gabrum (31 ± 1.9 mm), and A. niger (23 ± 1.7) had more ability for producing pectinase and cellulase. The anti-oxidant activity of isolates showed that A. alternata extract (IC50 = 471 ± 29 µg/mL) had the highest anti-oxidant properties, followed by A. tenuissima extract (IC50 = 512 ± 19 µg/mL). Also, the extract of A. alternata had the greatest amount of total phenols and flavonoids contents (8.2 ± 0.4 mg GAL/g and 2.3 ± 0.3 mg QE/g, respectively). The quantification analysis of phenolic acid showed that rosmarinic acid, para-coumaric acid, and meta-coumaric acid (42.02 ± 1.31, 7.53 ± 0.19, 5.41 ± 0.21 mg/g, respectively) were the main phenolic acids in the studied fungi. The analysis of fatty acids confirmed that, in all fungi, the main fatty acids were stearic acid (27.9–35.2%), oleic acid (11.3–17.3%), palmitic acid (16.9–23.2%), linoleic acid (5.8–11.6%), and caprylic acid (6.3–10.9%). Our finding showed that endophytic fungi are a source of bioactive compounds, which could be used in various industries. This is the first report of endophytic fungi associated with G. tournefortii, which provides knowledge on their future use on biotechnological processes.
Investigation of the rhythmic recruitment of tear neutrophils to the ocular surface and their phenotypes
Authors: Jin Yutong , Minten Ceili , Jenkins Mara , Jones Lyndon , Gorbet Maud
Published: March 2024

Hundreds of thousands of polymorphonuclear neutrophils (PMNs) are collected from the ocular surface upon waking, while few are harvested during daytime. This study aimed to investigate potential factors contributing to the circadian infiltration of tear PMNs, including changes in IL-8 and C5a in tears, and their phenotypes across different time points in a 24-h cycle. Tear PMNs were collected using a gentle eyewash after 2-h and 7-h of sleep (eye closure, EC) at night, after 2-h EC during the day, and towards the end of the afternoon. Significantly fewer cells were collected after 2-h EC during the day compared to 2-h EC at night. A positive correlation between IL-8 and PMN numbers existed, but not with C5a. Tear PMNs collected after 2-h EC at night were less degranulated and possessed a larger activation potential compared to 7-h EC. Tear PMNs from 7-h EC at night exhibited hyper-segmented nuclei and more NETosis compared to 2 h EC night, indicating an aged and activated phenotype. The diurnal-nocturnal recruitment pattern of tear PMNs may be driven by increased IL-8 in nighttime tears. Higher degranulation and NETs point to the significant activation of tear PMNs on the ocular surface during prolonged eye closure at night.
Effects of the interplay between topology and function of an integrated urban development on patterns of user movement
Authors: Manivannan Ajaykumar , Chin Wei Chien Benny , Gopalakrishnan Srilalitha , Wong Daniel K. H. , Schroepfer Thomas , Bouffanais Roland
Published: March 2024

With the advent of distributed multi-sensory networks of devices, vast troves of real-time data can be gathered about our interactions with the built environment. These rich data sets can be mined to achieve improved and informed data-driven designs of buildings, neighborhoods, and potentially entire cities. Among those, integrated developments have the peculiarity of combining multiple functions within a compact space and, as such, behave as microcosms of a city that can help address the problem of urban sprawl and density. However, a general lack of data and framework about integrated developments hinders our ability to test design hypotheses about the complex interplay between heterogeneity in both space and function. Here, we apply a data-driven approach to analyze the joint influence of topology and function on user movement within a state-of-the-art integrated development in Singapore. Specifically, we leverage the network representation of the building and use movement data collected from 51 individuals over a month. We show evidence of correlation (40%) between the spatial network features and human movement at the building level. We are also able to quantify the relationship between the functional and spatial components of the integrated development through user movement. Previous studies have shown a 60% or higher correlation between the topology and human movement at the city or country scales. Our moderate correlation, therefore, implies that more factors influencing user movement are at play. The heterogeneity in the spatial function introduced trips with diverse origins and destinations. A further data-driven analysis integrating origins and destinations reveals both qualitative and quantitative means of studying the relationship between the built environment and the processes that take place in them.
Copper(II) phosphate as a promising catalyst for the degradation of ciprofloxacin via photo-assisted Fenton-like process
Authors: Rozmyślak Mateusz , Walkowiak Adrian , Frankowski Marcin , Wolski Lukasz
Published: March 2024

This work aims to unravel the potential of copper(II) phosphate as a new promising heterogenous catalyst for the degradation of ciprofloxacin (CIP) in the presence of H2O2 and/or visible light (λ > 400 nm). For this purpose, copper(II) phosphate was prepared by a facile precipitation method and fully characterized. Of our particular interest was the elucidation of the kinetics of CIP degradation on the surface of this heterogeneous catalyst, identification of the main reactive oxygen species responsible for the oxidative degradation of CIP, and the evaluation of the degradation pathways of this model antibiotic pollutant. It was found that the degradation of the antibiotic proceeded according to the pseudo-first-order kinetics. Copper(II) phosphate exhibited ca. 7 times higher CIP degradation rate in a Fenton-like process than commercial CuO (0.00155 vs. 0.00023 min−1, respectively). Furthermore, the activity of this metal phosphate could be significantly improved upon exposure of the reaction medium to visible light (reaction rate = 0.00445 min−1). In a photo-assisted Fenton-like process, copper(II) phosphate exhibited the highest activity in CIP degradation from among all reference samples used in this study, including CuO, Fe2O3, CeO2 and other metal phosphates. The main active species responsible for the degradation of CIP were hydroxyl radicals.
An efficient intrusion detection model based on convolutional spiking neural network
Authors: Wang Zhen , Ghaleb Fuad A. , Zainal Anazida , Siraj Maheyzah Md , Lu Xing
Published: March 2024

Many intrusion detection techniques have been developed to ensure that the target system can function properly under the established rules. With the booming Internet of Things (IoT) applications, the resource-constrained nature of its devices makes it urgent to explore lightweight and high-performance intrusion detection models. Recent years have seen a particularly active application of deep learning (DL) techniques. The spiking neural network (SNN), a type of artificial intelligence that is associated with sparse computations and inherent temporal dynamics, has been viewed as a potential candidate for the next generation of DL. It should be noted, however, that current research into SNNs has largely focused on scenarios where limited computational resources and insufficient power sources are not considered. Consequently, even state-of-the-art SNN solutions tend to be inefficient. In this paper, a lightweight and effective detection model is proposed. With the help of rational algorithm design, the model integrates the advantages of SNNs as well as convolutional neural networks (CNNs). In addition to reducing resource usage, it maintains a high level of classification accuracy. The proposed model was evaluated against some current state-of-the-art models using a comprehensive set of metrics. Based on the experimental results, the model demonstrated improved adaptability to environments with limited computational resources and energy sources.
Surgical impact of bilateral transient occlusion of uterine and utero-ovarian arteries during laparoscopic myomectomy
Authors: Moratalla-Bartolomé Enrique , Lázaro-de-la-Fuente Jesús , López-Carrasco Irene , Cabezas-López Elena , Carugno Jose , Sancho-Sauco Javier , Pelayo-Delgado Irene
Published: March 2024

The objective of this article is to compare the amount of intraoperative blood loss during laparoscopic myomectomy when performing bilateral transient clamping of the uterine and utero-ovarian arteries versus no intervention. It´s a randomized controlled prospective study carried out in the Department of Obstetrics and Gynecology Ramón y Cajal University Hospital and HM Montepríncipe-Sanchinarro University Hospital, Madrid, Spain, in women with fibroid uterus undergoing laparoscopic myomectomy. Eighty women diagnosed with symptomatic fibroid uterus were randomly assigned to undergo laparoscopic myomectomy without additional intervention (Group A) or temporary clamping of bilateral uterine and utero-ovarian arteries prior to laparoscopic myomectomy (Group B). Estimated blood loss, operating time, length of hospital stay, and postoperative hemoglobin values were compared in both groups. The number of fibroids removed was similar in both groups (p = 0.77). Estimated blood loss was lower in the group of patients with prior occlusion of uterine arteries (p = 0.025) without increasing operating time (p = 0.17) nor length of stay (p = 0.17). No patient had either intra or postoperative complications. Only two patients (2.5%) required blood transfusion after surgery. We conclude that temporary clamping of bilateral uterine arteries prior to laparoscopic myomectomy is a safe intervention that reduces blood loss without increasing operative time.
Significance of pyrolytic temperature, application rate and incubation period of biochar in improving hydro-physical properties of calcareous sandy loam soil
Authors: Albalasmeh Ammar A. , Quzaih Mohammad Z. , Gharaibeh Mamoun A. , Rusan Munir , Mohawesh Osama E. , Rababah Samer R. , Alqudah Ahmad , Alghamdi Abdulaziz G. , Naserin Amir
Published: March 2024

Biochar is increasingly recognized for its ability to enhance hydro-physical properties of soil, offering promising solutions for improving soil structure, water retention, and overall agricultural productivity. In this study, sandy loam soil was amended at different rates (0, 15, 30, and 60 t ha−1) of biochar produced from olive pomace (Jift) at different pyrolysis temperatures (300, 400, 500, and 600 °C), and incubated for 30, 60, and 90 days. The biochar-amended soils were collected for analysis after each incubation period for infiltration rate, aggregate stability, soil water retention, water repellency, and penetration resistance. At 300 °C, aggregate stability increased with biochar amendments; the highest value (65%) was after 60 days of incubation. At other pyrolysis temperatures, aggregate stability decreased, or no effect of temperature was observed. Also, at 300 °C, the infiltration rate was decreased with biochar application and the lowest value of (0.14 ml/min) was at 90 days of incubation. At other pyrolysis temperatures, the infiltration rate was increased with increased biochar application rate. Water retention was increased with biochar application at 300 °C; however, biochar application did not affect water retention at other pyrolysis temperatures. These results strongly suggest the improvement of soil physical and hydraulic properties following the addition of biochar amendment. Overall, biochar had positive effects on hydro-physical properties. The biochar produced at 300 °C pyrolysis temperature was the most beneficial to agriculturally relevant hydraulic conditions. However, field assessments are necessary to evaluate the long-term effects of biochar on hydro-physical properties.
A randomized controlled study to assess the effect of mosapride citrate on intestinal recovery following gastrectomy
Authors: Jun Shiyeol , Oh Seyeol , Jung Ji Eun , Kwon In Gyu , Noh Sung Hoon
Published: March 2024

The enhanced recovery after surgery (ERAS) protocol, including prokinetic medications, is commonly used to prevent postoperative ileus. Prospective studies evaluating the effectiveness of mosapride citrate, a prokinetic 5-hydroxytryptamine 4 receptor agonist, in patients undergoing gastrectomy within the ERAS framework are lacking. This double-blind randomized trial included patients who were scheduled for laparoscopic or robotic gastrectomy for gastric cancer. Participants were randomly assigned to either a control (placebo) or experimental (mosapride citrate) group, with drugs administered on postoperative days 1–5. Bowel motility was evaluated based on bowel transit time measured using radiopaque markers, first-flatus time, and amount of food intake. No significant differences were observed in baseline characteristics between the two groups. On postoperative day 3, no significant difference was observed in the number of radiopaque markers visible in the colon between the groups. All factors associated with bowel recovery, including the time of first flatus, length of hospital stay, amount of food intake, and severity of abdominal discomfort, were similar between the two groups. Mosapride citrate does not benefit the recovery of intestinal motility after minimally invasive gastrectomy in patients with gastric cancer. Therefore, routine postoperative use of mosapride citrate is not recommended in such patients.
Integration of polygenic and gut metagenomic risk prediction for common diseases
Authors: Liu Yang , Ritchie Scott C. , Teo Shu Mei , Ruuskanen Matti O. , Kambur Oleg , Zhu Qiyun , Sanders Jon , Vázquez-Baeza Yoshiki , Verspoor Karin , Jousilahti Pekka , Lahti Leo , Niiranen Teemu , Salomaa Veikko , Havulinna Aki S. , Knight Rob , Méric Guillaume , Inouye Michael
Published: March 2024

Multiomics has shown promise in noninvasive risk profiling and early detection of various common diseases. In the present study, in a prospective population-based cohort with ~18 years of e-health record follow-up, we investigated the incremental and combined value of genomic and gut metagenomic risk assessment compared with conventional risk factors for predicting incident coronary artery disease (CAD), type 2 diabetes (T2D), Alzheimer disease and prostate cancer. We found that polygenic risk scores (PRSs) improved prediction over conventional risk factors for all diseases. Gut microbiome scores improved predictive capacity over baseline age for CAD, T2D and prostate cancer. Integrated risk models of PRSs, gut microbiome scores and conventional risk factors achieved the highest predictive performance for all diseases studied compared with models based on conventional risk factors alone. The present study demonstrates that integrated PRSs and gut metagenomic risk models improve the predictive value over conventional risk factors for common chronic diseases.