bluuebunny/crossref_metadata_2025 huggingface dataset:
A Ratiometric Fluorescent Probe for Hypochlorite and Lipid Droplets to Monitor Oxidative Stress
Authors: Baruah Mousumi , Jana Anal , Pareek Niharika , Singh Shikha , Samanta Animesh
Published: June 2023

Mitochondria are valuable subcellular organelles and play crucial roles in redox signaling in living cells. Substantial evidence proved that mitochondria are one of the critical sources of reactive oxygen species (ROS), and overproduction of ROS accompanies redox imbalance and cell immunity. Among ROS, hydrogen peroxide (H2O2) is the foremost redox regulator, which reacts with chloride ions in the presence of myeloperoxidase (MPO) to generate another biogenic redox molecule, hypochlorous acid (HOCl). These highly reactive ROS are the primary cause of damage to DNA (deoxyribonucleic acid), RNA (ribonucleic acid), and proteins, leading to various neuronal diseases and cell death. Cellular damage, related cell death, and oxidative stress are also associated with lysosomes which act as recycling units in the cytoplasm. Hence, simultaneous monitoring of multiple organelles using simple molecular probes is an exciting area of research that is yet to be explored. Significant evidence also suggests that oxidative stress induces the accumulation of lipid droplets in cells. Hence, monitoring redox biomolecules in mitochondria and lipid droplets in cells may give a new insight into cell damage, leading to cell death and related disease progressions. Herein, we developed simple hemicyanine-based small molecular probes with a boronic acid trigger. A fluorescent probe AB that could efficiently detect mitochondrial ROS, especially HOCl, and viscosity simultaneously. When the AB probe released phenylboronic acid after reacting with ROS, the product AB–OH exhibited ratiometric emissions depending on excitation. This AB–OH nicely translocates to lysosomes and efficiently monitors the lysosomal lipid droplets. Photoluminescence and confocal fluorescence imaging analysis suggest that AB and corresponding AB–OH molecules are potential chemical probes for studying oxidative stress.
Current Innovations in Intraocular Pressure Monitoring Biosensors for Diagnosis and Treatment of Glaucoma—Novel Strategies and Future Perspectives
Authors: Raveendran Rubiya , Prabakaran Lokesh , Senthil Rethinam , Yesudhason Beryl Vedha , Dharmalingam Sankari , Sathyaraj Weslen Vedakumari , Atchudan Raji
Published: June 2023

Biosensors are devices that quantify biologically significant information required for diverse applications, such as disease diagnosis, food safety, drug discovery and detection of environmental pollutants. Recent advancements in microfluidics, nanotechnology and electronics have led to the development of novel implantable and wearable biosensors for the expedient monitoring of diseases such as diabetes, glaucoma and cancer. Glaucoma is an ocular disease which ranks as the second leading cause for loss of vision. It is characterized by the increase in intraocular pressure (IOP) in human eyes, which results in irreversible blindness. Currently, the reduction of IOP is the only treatment used to manage glaucoma. However, the success rate of medicines used to treat glaucoma is quite minimal due to their curbed bioavailability and reduced therapeutic efficacy. The drugs must pass through various barriers to reach the intraocular space, which in turn serves as a major challenge in glaucoma treatment. Rapid progress has been observed in nano-drug delivery systems for the early diagnosis and prompt therapy of ocular diseases. This review gives a deep insight into the current advancements in the field of nanotechnology for detecting and treating glaucoma, as well as for the continuous monitoring of IOP. Various nanotechnology-based achievements, such as nanoparticle/nanofiber-based contact lenses and biosensors that can efficiently monitor IOP for the efficient detection of glaucoma, are also discussed.
BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays
Authors: Raysyan Anna , Zwigart Sandro , Eremin Sergei , Schneider Rudolf
Published: June 2023

BPA is a chemical commonly used in the production of polymer-based materials that can have detrimental effects on the thyroid gland and impact human reproductive health. Various expensive methods, such as liquid and gas chromatography, have been suggested for detecting BPA. The fluorescence polarization immunoassay (FPIA) is an inexpensive and efficient homogeneous mix-and-read method that allows for high-throughput screening. FPIA offers high specificity and sensitivity and can be carried out in a single phase within a timeframe of 20–30 min. In this study, new tracer molecules were designed that linked the fluorescein fluorophore with and without a spacer to the bisphenol A moiety. To assess the influence of the C6 spacer on the sensitivity of an assay based on the respective antibody, hapten–protein conjugates were synthesized and assessed for performance in an ELISA setup, and this resulted in a highly sensitive assay with a detection limit of 0.05 μg/L. The lowest limit of detection was reached by employing the spacer derivate in the FPIA and was 1.0 μg/L, working range from 2 to 155 μg/L. The validation of the methods was conducted using actual samples compared to LC–MS/MS, which served as the reference method. The FPIA and ELISA both demonstrated satisfactory concordance.
Unawareness of Apathy in Parkinson’s Disease: The Role of Executive Dysfunction on Symptom Recognition
Authors: Maggi Gianpaolo , Vitale Carmine , Delle Curti Alessia , Amboni Marianna , Santangelo Gabriella
Published: June 2023

Altered self-awareness or anosognosia may impact patients’ everyday life by interfering with their safe and independent functioning. Symptom awareness has been linked to executive dysfunctions caused by damage to frontal regions. Apathy is a frequent neuropsychiatric manifestation of Parkinson’s disease (PD) and is considered a consequence of altered functioning of cortico-subcortical circuitries connecting the prefrontal cortex (PFC) with the basal ganglia. Thus, apathetic PD patients may be not be fully aware of their condition due to shared neuropathophysiological mechanisms. The present study aimed to explore the awareness of apathy in PD patients by comparing the self-reported evaluations with their caregivers’ ratings. Moreover, we explored the clinical predictors of possible discrepancies and their consequences on patients’ self-reported evaluation of quality of life (QoL). We found a fair agreement between patients’ self-reports and caregivers’ ratings on apathy scores, with patients reporting less severe apathetic symptoms, especially those related to executive and auto-activation processing, compared to their caregivers’ reports. Executive functioning was found to mediate the relationship between disease stage and awareness of the apathetic state. Awareness of executive apathy impacted patients’ self-reported QoL. Therefore, PD patients might be unaware of their apathetic symptoms, especially those with worse executive functioning, which plays a key role in metacognitive processes such as self-monitoring and error detection. Anosognosia for apathy in PD patients may affect their QoL perception and leads to misleading self-report evaluations that delay diagnosis and treatment.
Visual Motor Reaction Times Predict Receptive and Expressive Language Development in Early School-Age Children
Authors: Alhamdan Areej A. , Murphy Melanie J. , Crewther Sheila G.
Published: June 2023

Proficiency of multisensory processing and motor skill are often associated with early cognitive, social, and language development. However, little research exists regarding the relationship between multisensory motor reaction times (MRTs) to auditory, visual and audiovisual stimuli, and classical measures of receptive language and expressive vocabulary development in school-age children. Thus, this study aimed to examine the concurrent development of performance in classical tests of receptive (Peabody Picture Vocabulary Test; abbreviated as PPVT) and expressive vocabulary (Expressive Vocabulary Test; abbreviated as EVT), nonverbal intelligence (NVIQ) (determined with the aid of Raven’s Colored Progressive Matrices; abbreviated as RCPM), speed of visual–verbal processing in the Rapid Automatic Naming (RAN) test, Eye–Hand Co-ordination (EHC) in the SLURP task, and multisensory MRTs, in children (n = 75), aged between 5 and 10 years. Bayesian statistical analysis showed evidence for age group differences in EVT performance, while PPVT was only different for the youngest group of children aged 5–6, supporting different developmental trajectories in vocabulary acquisition. Bayesian correlations revealed evidence for associations between age, NVIQ, and vocabulary measures, with decisive evidence and a higher correlation (r = 0.57 to 0.68) between EVT, MRT tasks, and EHC visuomotor processing. This was further supported by regression analyses indicating that EVT performance was the strongest unique predictor of multisensory MRTs, EHC, and RAN time. Additionally, visual MRTs were found to predict both receptive and expressive vocabulary. The findings of the study have important implications as accessible school-based assessments of the concurrent development of NVIQ, language, and multisensory processing; and hence as rapid and timely measures of developmental and neurodevelopmental status.
Evaluating Technology-Based Self-Monitoring of Performance with Differential Reinforcement for Students with Disabilities
Authors: Risse Madeline R. , Blair Kwang-Sun Cho , Russo Danielle A.
Published: June 2023

This study evaluated the use of a technology-based self-monitoring of performance (SMP) with differential reinforcement to increase task completion and reduce off-task behavior for three 5th-grade students with disabilities. A concurrent multiple baseline design across participants was used to examine the impact of the intervention on the targeted behaviors when implemented by a general education teacher and its maintenance effects with a delay of reinforcement. The implementation involved training students to use a mobile app for SMP and providing differential reinforcement contingent on task completion and accuracy of self-monitoring during academic periods. The secondary measure of off-task behavior was included to evaluate the relationship between task completion and engagement. The results demonstrated that the technology-based SMP with differential reinforcement increased task completion and reduced off-task behavior for all students. Furthermore, the gradual fading of the reinforcement, with a 45 min delay, was successful for all students. The efficiency and immediacy of the intervention suggest that technology-based SMP with differential reinforcement holds promise as a practical, efficient, and effective school-based intervention.
Experimental Study on Mechanical Properties of Structured Clay under Different Unloading Rates and Unloading Stress Paths
Authors: Li Lu , Zang Meng , Zhang Rongtang , Lu Haijun
Published: June 2023

Consolidated undrained triaxial shear tests were performed on undisturbed saturated structured clay at three unloading rates (0.1, 0.25, and 2.5 kPa/min) using a GDS triaxial system to determine the effects of different unloading rates and unloading stress paths on the stress–strain relationship, pore pressure variation, and failure strength characteristics of Zhanjiang structured clay. Microstructural changes in the clay were observed during shear tests at different unloading rates. Furthermore, the obtained stress–strain relationship indicates strain-softening under different unloading stress paths. Under the same axial strain, a larger unloading rate caused a larger deviatoric stress. Under the same conditions, the higher the confining pressure, the greater the peak pore pressure, the smaller the unloading rate, the greater the pore pressure development, and the greater the variation in the pore pressure. Moreover, the undrained shear strength increased with an increase in the unloading rate from 0.1 to 2.5 kPa/min. The change in the unloading rate had a greater effect on the undrained strength under the passive tensile path than that under the passive compression path. The microstructure of the Zhanjiang structured clay changed after shear tests at different unloading rates, exhibiting various degrees of adjustment in the particle arrangement, contact relations, pore sizes, and shapes.
Nonlinear Dynamic Assessment of a Steel Frame Structure Subjected to Truck Collision
Authors: Safari Honar Fatemeh , Broujerdian Vahid , Mohammadi Dehcheshmeh Esmaeil , Bedon Chiara
Published: June 2023

The progressive collapse of structures subjected to a truck collision with ground floor columns is numerically investigated in this paper. For this purpose, a four-story steel building with a dual system (including an intermediate steel moment frame, with a special concentric steel bracing system in the longitudinal (x) direction, and an intermediate steel moment frame in the transversal (y) direction) is considered. The structure, which was designed according to AISC, ASCE7 and 2800 Iranian seismic standard guidelines, is located in seismic-prone area and subjected to eight different truck collision scenarios. The nonlinear dynamic analyses carried out in ABAQUS on a three-dimensional finite element (FE) numerical model include variations in collision features (i.e., mass and speed of the truck, the height of collision point), and are used to support the analysis of expected damage. The presented results confirm that increasing the truck mass and speed increases damage entity for the column and structure. Several influencing parameters are involved in damage location and progressive evolution. The height of the collision point from the ground also significantly affects the magnitude of structural damage, especially in terms of stress peaks in the panel zones for the target column. Finally, the perimeter columns are more vulnerable to impact than corner columns, in structures with dual system as with the examined four-story building. The presence of a bracing system parallel to the impacting vehicle can in fact reduce the deformation—and thus the expected damage—of the adjacent target column. Most importantly, it is shown that the numerically reproduced collision scenarios (and the associated damage configurations) based on truck impact are significantly more severe than those artificially created based on the conventional column removal method (i.e., alternate path (AP) analysis approach), which confirms the importance of more sophisticated numerical calculation procedures to investigate and assess the progressive collapse of structures.
Potential Use of Oyster Shell Waste in the Composition of Construction Composites: A Review
Authors: Bellei Poliana , Torres Isabel , Solstad Runar , Flores-Colen Inês
Published: June 2023

The oyster shell is a residue rich in calcium carbonate, which can be reused as a raw material for creating building materials. For this reason, many researchers focused on the incorporation of oyster shell in the composition of composites, as it is a means of contributing to the economic sustainability by reducing the presence of pollution caused by aquaculture waste in the environment, thus increasing the value chain of the construction sector and reducing its carbon footprint. This paper intends to systematize the scientific production related to oyster shell-based composites in construction, carrying out a search using the Scopus tool and a systematic review based on the PRISMA statement. The results show that research on the incorporation of oyster shell into cementitious mortar mixtures, with a focus on its use in concrete, dominates existing scientific research. There is a lack of studies on the incorporation of the oyster shell that address its application as an aggregate or binder in the composition of coating and laying mortars. Most existing research is from Asia, and there is a lack of research in some parts of Europe. In the Americas, Africa and Oceania, no existing studies were found. Despite the growing understanding of the importance of sustainability and economic issues related to products used in the blue circular economy sector, there are still few studies that consider the incorporation of waste or by-products of aquaculture. Future investigations that cover these practical and contextual gaps can contribute to the better use of oyster shell waste and its insertion in the blue circular economy.
Numerical and Experimental Study on Loading Behavior of Facade Sandwich Panels
Authors: Stanisavljević Gorjana , Golubović Matić Darinka , Komnenović Milorad , Vasović Maksimović Ivana , Flajs Željko
Published: June 2023

This paper focuses on the study of the strength of facade sandwich panels used in building construction. The paper describes the results of experimental and numerical research on the behavior of sandwich panels made of polyisocyanurate core (PIR) and their structural connections when exposed to tensile and compressive loads. In the initial phase of this study, laboratory tests were performed to determine the physical and mechanical characteristics of the material from which the sandwich panels are made. Laboratory tensile and compression tests were performed on small samples of sandwich facade panels. In order to verify the obtained results, they were compared with the numerical analysis performed in the ANSYS software. The numerical model was found to accurately predict the results of the laboratory tests, suggesting that the model can be used to predict the behavior of these panels under different loads in service. The study showed that the foam core sandwich panel exhibits excellent mechanical properties. The results indicate the suitability of foam-based composite structures in the construction industry for various applications, such as roof and wall structures. The findings of this study may help in the development of lightweight and durable construction materials for the industry.
From “Division” to “Integration”: Evolution and Reform of China’s Spatial Planning System
Authors: Zhang Yongjiao , Man Xiaowu , Zhang Yongnian
Published: June 2023

Spatial planning is a public policy arrangement for land use allocation and spatial structure regulation. As a method used by the public sector to influence the spatial distribution of future activities, spatial planning has become an important method and basis for the Chinese government to perform its duties. In the process of its long-term development, China has formed a unique spatial planning system. Based on the perspective of evolution and comparison, this paper systematically reviews the evolution of China’s spatial planning system from “multi-plan division” to “multi-plan integration” under the inheritance of departments. The findings are as follows. ① China’s spatial planning has long presented a pattern of separate management by multiple departments, such as development and reform, construction, land, and environmental protection. The emergence and development of various types of planning is a necessary spatial governance tool for specific national conditions and major issues of land space development and protection in China. ② In the evolution process of more than half a century, the planning of various departments has gradually established, inherited, and continuously changed their own planning systems and control content; thus, China’s spatial planning has undergone a process of “planning absence–planning division–planning integration”. ③ The brand-new territorial spatial plan inherits the “three types” of control space, including land utilization master planning, urban and rural master planning, and ecological environment planning, and forms a set of binding index systems, which have become the decision-making basis for the current territorial space resource allocation. ④ In the future, China’s spatial planning system should be further optimized and improved in aspects such as the coordination mechanism of “soft” and “hard” spatial planning, the spatial resource allocation system that places equal emphasis on legality and efficiency, and the spatial layout system from “major function-oriented zoning” to “space use zoning”. Insight into the evolution of China’s spatial planning system can provide historical and logical support for the improvement of China’s spatial governance thinking and the continuous improvement of the efficiency of land space resource allocation in the future and provide a certain reference value for the comparative study of the planning systems of different countries in the world.
Analysis of Carbon Emission Reduction Paths for the Production of Prefabricated Building Components Based on Evolutionary Game Theory
Authors: Wang Qun , Guo Wei , Xu Xizhen , Deng Ronghui , Ding Xiaoxin , Chen Tiebing
Published: June 2023

Prefabricated buildings are gradually being promoted from pilot demonstration to scale, to combat climate change and improve energy conservation and emission reduction in the building sector. Despite the carbon emission of assembled structures being substantially lower than that of cast-in-place buildings due to the significant reduction in energy demand during the materialization process, there is still a lot of room for improvement. This study looks at the strategy choices made by manufacturers of prefabricated building components in relation to lowering carbon emissions from the standpoint of manufacturing prefabricated building components. By building a dynamic evolutionary game model between two parties, we investigate the evolutionary process of the strategy chosen by prefabricated building component manufacturers and the government, analyzing the evolutionary stability of each side’s strategy choice, and finally using Matlab tools to simulate the effectiveness of the evolutionary stability. The study findings indicate that (1) low-carbon production costs, local government incentives and sanctions, and corporate low-carbon production benefits are the main influencing factors for manufacturers of prefabricated building components to adopt low-carbon production techniques; and that (2) the cost of regulation under low-carbon production methods and the local government performance assessment system are the key elements affecting regulations by the local government. Based on this finding, we suggest corresponding countermeasures in three areas, including investigating new low-carbon technology options for businesses, developing a new carbon emission accounting subsidy mechanism, and improving the regulatory framework of the government, to provide an efficient pathway for the growth of a low-carbon economy.
The Distinctive Features behind the Aggressiveness of Oral and Cutaneous Squamous Cell Carcinomas
Authors: Alonso-Juarranz Miguel , Mascaraque Marta , Carrasco Elisa , Gracia-Cazaña Tamara , De La Sen Oscar , Gilaberte Yolanda , Gonzalez Salvador , Juarranz Ángeles , Falahat Farzin
Published: June 2023

Squamous cell carcinomas arise from stratified squamous epithelia. Here, a comparative analysis based on recent studies defining the genetic alterations and composition of the stroma of oral and cutaneous squamous cell carcinomas (OSCC and CSCC, respectively) was performed. Both carcinomas share some but not all histological and genetic features. This review was focused on how mutations in tumor suppressor genes and protooncogenes cooperate to determine the differentiation, aggressiveness, and metastatic potential of OSCC and CSCC. In fact, driver mutations in tumor suppressor genes are more frequently observed in OSCC than CSCC. These include mutations in TP53 (encoding pP53 protein), CDKN2A (encoding cyclin dependent kinase inhibitor 2A), FAT1 (encoding FAT atypical cadherin 1), and KMT2D (encoding lysine methyltransferase 2D), with the exception of NOTCH (encoding Notch receptor 1), whose mutation frequency is lower in OSCC compared to CSCC. Finally, we describe the differential composition of the tumor microenvironment and how this influences the aggressiveness of each tumor type. Although both OSCC and CSCC tumors are highly infiltrated by immune cells, high levels of tumor-infiltrating lymphocytes (TILs) have been more frequently reported as predictors of better outcomes in OSCC than CSCC. In conclusion, OSCC and CSCC partially share genetic alterations and possess different causal factors triggering their development. The tumor microenvironment plays a key role determining the outcome of the disease.
Fat-Containing Soft Tissue Tumors in Children, Adolescents, and Young Adults: Which Require Biopsy?
Authors: Cardoen Liesbeth , Nicolas Nayla , Le Gaudu Violette , Gauthier Arnaud , Carton Matthieu , Berrebi Dominique , Cyrta Joanna , Collignon Charlotte , Cordero Camille , Pierron Gaëlle , Pannier Stéphanie , Philippe-Chomette Pascale , Orbach Daniel , Brisse Hervé J.
Published: June 2023

Purpose: To confirm the overall benignity of fat-containing soft tissue tumors (STT) on a pediatric cohort and to define the clinical and imaging features that warrant a biopsy. Methods: A retrospective monocentric study was conducted on patients aged less than 25 years consecutively referred for fat-containing STT to our Comprehensive Cancer Center between 1998 and 2022. Tumor imaging characteristics at diagnosis (US, CT, or MRI) were correlated with pathology. Results: The database extraction identified 63 fat-containing tumors with clinical, histologic, and imaging data available for review. In total, 58 (92%) were benign tumors: 36 lipoblastomas and lipomas, 12 fibrous hamartomas of infancy (FHI), 5 lipofibromatosis, 2 lipomas arborescens, 2 lipomatosis and 1 spindle-cell lipoma. Five patients (8%) were diagnosed with liposarcoma. Factors significantly correlated with malignancy were age >10 years old (p < 0.001), having a cancer-predisposing condition (p < 0.001), a percentage of fat <25% (p = 0.002), and a presence of myxoid zones (p < 0.001) on imaging. Conclusion: Most fat-containing STT in children may be classified as benign tumors based on clinics and imaging. The indication for biopsy could be limited to patients aged 10 years or more with either a cancer-predisposing condition or imaging features demonstrating either a low-fat component (<25%) or the presence of myxoid zones.
Comparative Analyses of the Clinicopathologic Features of Short-Term and Long-Term Survivors of Patients with Pancreatic Ductal Adenocarcinoma Who Received Neoadjuvant Therapy and Pancreatoduodenectomy
Authors: Liang Tom Z. , Katz Matthew H. G. , Prakash Laura R. , Chatterjee Deyali , Wang Hua , Kim Michael , Tzeng Ching-Wei D. , Ikoma Naruhiko , Wolff Robert A. , Zhao Dan , Koay Eugene J. , Maitra Anirban , Kundu Suprateek , Wang Huamin
Published: June 2023

Neoadjuvant therapy (NAT) is increasingly used to treat patients with pancreatic ductal adenocarcinoma (PDAC). Patients with PDAC often show heterogenous responses to NAT with variable clinical outcomes, and the clinicopathologic parameters associated with these variable outcomes remain unclear. In this study, we systematically examined the clinicopathologic characteristics of 60 short-term survivors (overall survival < 15 months) and 149 long-term survivors (overall survival > 60 months) and compared them to 352 intermediate-term survivors (overall survival: 15–60 months) of PDAC who received NAT and pancreatoduodenectomy. We found that the short-term survivor group was associated with male gender (p = 0.03), tumor resectability prior to NAT (p = 0.04), poorly differentiated tumor histology (p = 0.006), more positive lymph nodes (p = 0.04), higher ypN stage (p = 0.002), and higher positive lymph node ratio (p = 0.03). The long-term survivor group had smaller tumor size (p = 0.001), lower ypT stage (p = 0.001), fewer positive lymph nodes (p < 0.001), lower ypN stage (p < 0.001), lower positive lymph node ratio (p < 0.001), lower rate of lymphovascular invasion (p = 0.001) and perineural invasion (p < 0.001), better tumor response grading (p < 0.001), and less frequent recurrence/metastasis (p < 0.001). The ypN stage is an independent predictor of both short-term and long-term survivors by multivariate logistic regression analyses. In addition, tumor differentiation was also an independent predictor for short-term survivors, and tumor response grading and perineural invasion were independent predictors for long-term survivors. Our results may help to plan and select post-operative adjuvant therapy for patients with PDAC who received NAT and pancreatoduodenectomy based on the pathologic data.
Effect of Mitotane on Male Gonadal Function
Authors: Innocenti Federica , Di Persio Sara , Taggi Marilena , Maggio Roberta , Lardo Pina , Toscano Vincenzo , Canipari Rita , Vicini Elena , Stigliano Antonio
Published: June 2023

Background: Clinical evidence has shown frequent hypogonadism following mitotane (MTT) treatment in male patients with adrenocortical carcinoma. This study aimed to evaluate the impact of MTT on male gonadal function. Methods: Morphological analysis of testes and testosterone assays were performed on adult CD1 MTT-treated and untreated mice. The expression of key genes involved in interstitial and tubular compartments was studied by real-time PCR. Moreover, quantitative and qualitative analysis of spermatozoa was performed. Results: Several degrees of damage to the testes and a significant testosterone reduction in MTT-treated mice were observed. A significant decline in 3βHsd1 and Insl3 mRNA expression in the interstitial compartment confirmed an impairment of androgen production. Fsh-R mRNA expression was unaffected by MTT, proving that Sertoli cells are not the drug’s primary target. Sperm concentrations were significantly lower in MTT-treated animals. Moreover, the drug caused a significant increase in the percentage of spermatozoa with abnormal chromatin structures. Conclusion: MTT negatively affects the male reproductive system, including changes in the morphology of testicular tissue and reductions in sperm concentration and quality.
Full-Dose Intraoperative Electron Radiotherapy for Early Breast Cancer: Evidence from a Single Center’s Experience
Authors: Stefanelli Antonio , Farina Eleonora , Mastella Edoardo , Fabbri Sara , Turra Alessandro , Bonazza Simona , De Troia Alessandro , Radica Margherita K. , Carcoforo Paolo
Published: June 2023

To evaluate the clinical response rate and cosmetic outcome after full-dose intraoperative electron radiotherapy (IOERT) in early breast cancer (BC) treated with conserving surgery. Inclusion criteria were: >60 years old, clinical tumor size ≤2 cm, luminal A carcinoma, patological negative lymph nodes, excluded lobular carcinoma histology. IOERT was delivered with a dose of 21 Gy at 90% isodose. Clinical, cosmetic and/or instrumental follow-up were performed 45 days after IOERT, 6 months after the first check, and every 12 months thereafter. Acute and late toxicities were assessed with the CTCAE v.4.03 and EORTC-RTOG scales, respectively. Cosmetic outcome was evaluated using the Harvard/NSABO/RTOG Breast Cosmesis Grading Scale. Overall, 162 consecutive patients were included in this analysis (median follow-up: 54 months, range: 1–98 months). The overall response rate was 97.5% (CI 95%: 0.93–0.99%). Locoragional relapse occurred in 2.5% of patients. No patient showed distant metastases. No patient showed radiation-related acute complications, with 3.7% showing late G2–3 toxicity. Only 3.7% of patients showed poor cosmetic results. Our data confirmed that IOERT is a feasible and valid therapeutic option in low-risk BC patients treated with lumpectomy. A low local recurrence rate combined with good cosmetic results validates the settings of our operative method in routinely clinical practice.
Adenovirus-Derived Nano-Capsid Platforms for Targeted Delivery and Penetration of Macromolecules into Resistant and Metastatic Tumors
Authors: Benhaghnazar Rebecca , Medina-Kauwe Lali
Published: June 2023

Macromolecular therapeutics such as nucleic acids, peptides, and proteins have the potential to overcome treatment barriers for cancer. For example, nucleic acid or peptide biologics may offer an alternative strategy for attacking otherwise undruggable therapeutic targets such as transcription factors and similar oncologic drivers. Delivery of biological therapeutics into tumor cells requires a robust system of cell penetration to access therapeutic targets within the cell interior. A highly effective means of accomplishing this may be borrowed from cell-penetrating pathogens such as viruses. In particular, the cell entry function of the adenovirus penton base capsid protein has been effective at penetrating tumor cells for the intracellular deposition of macromolecular therapies and membrane-impermeable drugs. Here, we provide an overview describing the evolution of tumor-targeted penton-base-derived nano-capsids as a framework for discussing the requirements for overcoming key barriers to macromolecular delivery. The development and pre-clinical testing of these proteins for therapeutic delivery has begun to also uncover the elusive mechanism underlying the membrane-penetrating function of the penton base. An understanding of this mechanism may unlock the potential for macromolecular therapeutics to be effectively delivered into cancer cells and to provide a treatment option for tumors resisting current clinical therapies.
LIN28B and Let-7 in Diffuse Midline Glioma: A Review
Authors: Knowles Truman , Huang Tina , Qi Jin , An Shejuan , Burket Noah , Cooper Scott , Nazarian Javad , Saratsis Amanda M.
Published: June 2023

Diffuse midline glioma (DMG) is the most lethal of all childhood cancers. DMGs are driven by histone-tail-mutation-mediated epigenetic dysregulation and partner mutations in genes controlling proliferation and migration. One result of this epigenetic and genetic landscape is the overexpression of LIN28B RNA binding protein. In other systems, LIN28B has been shown to prevent let-7 microRNA biogenesis; however, let-7, when available, faithfully suppresses tumorigenic pathways and induces cellular maturation by preventing the translation of numerous oncogenes. Here, we review the current literature on LIN28A/B and the let-7 family and describe their role in gliomagenesis. Future research is then recommended, with a focus on the mechanisms of LIN28B overexpression and localization in DMG.
Electrochemical Promotion of CO2 Hydrogenation Using Rh Catalysts Supported on O2− Conducting Solid Electrolyte
Authors: Kokkinou Nikoleta , Xydas Fotios , Brosda Susanne , Kyriakou Georgios , Katsaounis Alexandros
Published: June 2023

Electrochemical promotion was used to modify the activity and selectivity of a Rh catalyst electrode in the CO2 hydrogenation reaction. The experiments were carried out in a temperature range of 350–430 °C at ambient pressure and at different CO2 to H2 gas feeding ratios (1:2 to 4:1). The only reaction products observed were CO and CH4, both under open- and closed-circuit conditions. The CH4 formation rate was found to increase with both positive and negative potential or current application. The CO formation rate followed the opposite trend. The selectivity to CH4 increased under high values of hydrogen partial pressure and decreased at high pressures of CO2. The results demonstrate how electrochemical promotion can be used to finely tune activity and selectivity for a reaction of high technical and environmental importance.
Gas-Phase Deoxygenation of Biomass Pyrolysis Tar Catalyzed by Rare Earth Metal Loaded Hβ Zeolite
Authors: Jazie Ali A. , Haydary Juma , Abed Suhad A. , Husár Jakub
Published: June 2023

Biomass pyrolysis tar (BPT) with a higher heating value of 24.23 MJ/kg was used as raw feed for the catalytic gas-phase deoxygenation (GDO) process using Hβ zeolite loaded with different amounts of active elements (Ce, La, and Nd). Acetone molecule was chosen as a model compound to test the activity of pure Hβ zeolite, 1 wt% Ce/Hβ zeolite, 5 wt% Ce/Hβ zeolite, 1 wt% La/Hβ zeolite, 5 wt% La/Hβ zeolite, 1 wt% Nd/Hβ zeolite, and 5 wt% Nd/Hβ zeolite at 400 °C and process time of 3 h. BPT characterization showed a wide range of oxygenated compounds with the main components including water: 0.71%, furfural: 5.85%, 4-ethylguaiacol: 2.14%, phenol: 13.63%, methylethyl ketone: 5.34%, cyclohexanone: 3.23%, isopropanol: 4.78%, ethanol: 3.67%, methanol: 3.13%, acetic acid: 41.06%, and acetone: 16.46%. BPT conversion using 1 wt% Ce/Hβ zeolite catalyst showed the highest values of degree of deoxygenation (DOD) (68%) and conversion (16% for phenol, 88% for acetic acid, and 38% for 4-ethlyguaiacol). Yields of water, liquid phase, and gas phase in the GDO reaction using 1%Ce/Hβ zeolite were 18.33%, 47.42%, and 34.25%, respectively. Alkyl-substituted phenols and aromatic hydrocarbons achieved the highest yields of 37.34% and 35.56%, respectively. The main interaction pathways for BPT-GDO are also proposed.
The Role of RNA Sensors in Regulating Innate Immunity to Gammaherpesviral Infections
Authors: Zhang Huirong , Sandhu Praneet K. , Damania Blossom
Published: June 2023

Kaposi’s sarcoma-associated herpesvirus (KSHV) and the Epstein–Barr virus (EBV) are double-stranded DNA oncogenic gammaherpesviruses. These two viruses are associated with multiple human malignancies, including both B and T cell lymphomas, as well as epithelial- and endothelial-derived cancers. KSHV and EBV establish a life-long latent infection in the human host with intermittent periods of lytic replication. Infection with these viruses induce the expression of both viral and host RNA transcripts and activates several RNA sensors including RIG-I-like receptors (RLRs), Toll-like receptors (TLRs), protein kinase R (PKR) and adenosine deaminases acting on RNA (ADAR1). Activation of these RNA sensors induces the innate immune response to antagonize the virus. To counteract this, KSHV and EBV utilize both viral and cellular proteins to block the innate immune pathways and facilitate their own infection. In this review, we summarize how gammaherpesviral infections activate RNA sensors and induce their downstream signaling cascade, as well as how these viruses evade the antiviral signaling pathways to successfully establish latent infection and undergo lytic reactivation.
Mechanisms of Atrial Fibrillation in Obstructive Sleep Apnoea
Authors: Saleeb-Mousa James , Nathanael Demitris , Coney Andrew M. , Kalla Manish , Brain Keith L. , Holmes Andrew P.
Published: June 2023

Obstructive sleep apnoea (OSA) is a strong independent risk factor for atrial fibrillation (AF). Emerging clinical data cite adverse effects of OSA on AF induction, maintenance, disease severity, and responsiveness to treatment. Prevention using continuous positive airway pressure (CPAP) is effective in some groups but is limited by its poor compliance. Thus, an improved understanding of the underlying arrhythmogenic mechanisms will facilitate the development of novel therapies and/or better selection of those currently available to complement CPAP in alleviating the burden of AF in OSA. Arrhythmogenesis in OSA is a multifactorial process characterised by a combination of acute atrial stimulation on a background of chronic electrical, structural, and autonomic remodelling. Chronic intermittent hypoxia (CIH), a key feature of OSA, is associated with long-term adaptive changes in myocyte ion channel currents, sensitising the atria to episodic bursts of autonomic reflex activity. CIH is also a potent driver of inflammatory and hypoxic stress, leading to fibrosis, connexin downregulation, and conduction slowing. Atrial stretch is brought about by negative thoracic pressure (NTP) swings during apnoea, promoting further chronic structural remodelling, as well as acutely dysregulating calcium handling and electrical function. Here, we provide an up-to-date review of these topical mechanistic insights and their roles in arrhythmia.
Disaster Risk, Climate Change, and Urbanization as Research Topics in Western Asia—A Bibliometric Literature Analysis
Authors: Fekete Alexander
Published: June 2023

Scientifically analyzing and documenting climate change and related disaster risks is demanded by international organizations such as the United Nations. However, global or national studies predominate, and cross-regional overviews are lacking, especially for Western Asia. In four countries in the region, Iran, Israel, Saudi Arabia, and Turkey, transport accidents, floods, fires, and earthquakes are the predominant accidents and disasters in the Emergency Events Database (EM-DAT). The result is different when analyzing the scientific publications via a bibliometric literature analysis using VOS viewer and the Web of Science, and earthquakes, climate change, COVID-19, and terrorism dominate here. Governance and management are also an important and recurring cluster topic. The conceptual components of vulnerability and resilience are discussed in most countries. The hazards are often associated with specific concepts and quantitative methods. GIS and remote sensing as specific methodologies also often appear in a cluster. Further clusters derived from the keyword search include floods and droughts, food security and agriculture, and posttraumatic stress and psychological aspects. The results help us to identify countries with a rich literature on certain hazards and gaps in relation to other types of disasters, which are more prevalent. The findings can help scientists and policymakers to support future studies based on either high or low research coverage.
The Design and Preparation of Antibacterial Polymer Brushes with Phthalocyanine Pigments
Authors: Zhou Yu , Chen Kaimin , Liu Li , Wen Shaoguo , Gui Taijiang
Published: June 2023

Phthalocyanine pigments have many problems in waterborne coating applications because of their low polarity, poor dispersion in water, and easy agglomeration properties. In order to solve these problems, the phthalocyanine pigments were encapsulated with a copolymer of methyl methacrylate (MMA) and butyl acrylate (BA) by a mini-emulsion polymerization method. The pigments are effectively dispersed in water and have good compatibility with the resin. Concerning the bacterial reproduction and growth problem for the waterborne system, the resin-encapsulated phthalocyanine pigments were further grafted with antibacterial polymer poly(N-(2-hydroxyethyl) acrylamide) (PHEAA) on its surface using the photoemulsion polymerization technique. Comprehensive properties, including centrifugal stability and chromaticity change, were studied. The polymer encapsulation improved the centrifugal stability of the pigment. The thermogravimetric results showed that the residual mass of C.I. Pigment Green 7 (52.30%) was higher than that of C.I. Pigment Blue 15:3 (30.06%), and the sublimation fastness of PG7 was higher. The TEM results revealed that the shape of the PG7 after encapsulation and grafting was more regular than that of PB15:3. The L* of the pigment decreased after encapsulation but then increased after further grafting. The phthalocyanine pigment composite latex had good antibacterial properties after the grafting of PHEAA.
Effect of Aging Temperature on Precipitates Evolution and Mechanical Properties of GH4169 Superalloy
Authors: Liu Anqi , Zhao Fei , Huang Wensen , Tan Yuanbiao , Ren Yonghai , Wang Longxiang , Xu Fahong
Published: June 2023

GH4169 is primarily strengthened through precipitation, with heat treatment serving as a crucial method for regulating the precipitates of the alloy. However, the impact of aging temperature on the microstructure and properties of GH4169 has not been thoroughly studied, hindering effective regulation of its microstructure and properties. This study systematically investigated the effects of aging temperature on the evolution of precipitates and mechanical properties of GH4169 alloy using various techniques such as OM, SEM, XRD and TEM. The results indicate that raising the aging temperature leads to an increase in the sizes of both the γ″ and γ′ phases in the alloy, as well as promoting the precipitation of δ phase at grain boundaries. Notably, the increase in γ″ phase size enhances the strength of the alloy, while the presence of δ phase is detrimental to its strength but greatly enhances its elongation. The yield strength of the alloy aged at 750 ℃ exhibits the highest yield strength, with values of 1135 MPa and 1050 MPa at room temperature and elevated temperature, respectively. As the aging temperature increases, the Portevin-Le Châtelier (PLC) effect during elevated temperature tensile tests at 650 ℃ gradually weakens. The PLC effect disappears almost completely when the aging temperature reaches 780 ℃.
Photoaligned Liquid Crystalline Structures for Photonic Applications
Authors: Kudreyko Aleksey , Chigrinov Vladimir , Hegde Gurumurthy , Chausov Denis
Published: June 2023

With the advancement of information display technologies, research on liquid crystals is undergoing a tremendous shift to photonic devices. For example, devices and configurations based on liquid crystal materials are being developed for various applications, such as spectroscopy, imaging, and fiber optics. One of the problems behind the development of photonic devices lies in the preparation of patterned surfaces that can provide high resolution. Among all liquid crystal alignment techniques, photoalignment represents a promising non-contact method for the fabrication of patterned surfaces. In this review, we discuss the original research findings on electro-optic effects, which were mainly achieved at the Department of Electronic and Computer Engineering of the Hong Kong University of Science and Technology and the collaborating research laboratories.
Enhanced Performances of Quantum Dot Light-Emitting Diodes with an Organic–Inorganic Hybrid Hole Injection Layer
Authors: Chen Ling , Jiang Donghuai , Du Wenjing , Shang Jifang , Li Dongdong , Liu Shaohui
Published: June 2023

PEDOT:PSS (polyethylene dioxythiophene:polystyrenesulfonate) is a commonly used hole injection layer (HIL) in optoelectronic devices due to its high conductive properties and work function. However, the acidic and hygroscopic nature of PEDOT:PSS can be problematic for device stability over time. To address this issue, in this study we demonstrated the potential of an organic–inorganic hybrid HIL by incorporating solution-processed WOx nanoparticles (WOx NPs) into the PEDOT:PSS mixture. This hybrid solution was found to have a superior hole transport ability and low Ohmic contact resistance contributing to higher brightness (~62,000 cd m−2) and current efficiency (13.1 cd A−1) in the manufactured quantum-dot-based light-emitting diodes (QLEDs). In addition, the resulting devices achieved a relative operational lifetime of 7071 h, or approximately twice that of traditional QLEDs with PEDOT:PSS HILs. The proposed method is an uncomplicated, reliable, and low-cost way to achieve long operational lifetimes without sacrificing efficiency in optoelectronic devices.