bluuebunny/crossref_metadata_2025 huggingface dataset:
Service as an innovation
Authors: Zavodna Lucie Sara
Published: December 2021

Service innovations make life of customers easier during or after use of company’s products or services. They are coming in different forms. Companies create strong connections with their customers by offering different helps, consultations or new experience. Service innovations make customers’ experiences stronger and strengthen their gratitude. This chapter provides two case studies. The first case study is the famous company Whirlpool, which made service innovation by implementing CRM system. This enabled stronger straight relationship with their final customers and expansion of incomes. The second case study is an innovative way of selling old antiquarian books in the Czech company Knihobot. This innovation enables fast preparation of books for selling and introduces automation into a new area.
Storytelling in business—How to increase customer engagement
Authors: Olejnik Iwona
Published: December 2021

Storytelling, as a marketing innovation, is the market communication tool that allows present the facts in a coherent and attractive way so as to stand out from the competition and more effectively influence the consumer’s imagination and engagement. It can be concluded that the history of storytelling itself dates back to the beginnings of mankind, when humans started to transfer knowledge from generation to generation. The chapter indicates that storytelling evokes emotions in customers, and these in turn affect commitment and action. The most important and primary elements of storytelling include superior message, conflict, characters (the archetypes) and plot. Each good story can be based on a few important points, which are well described by the concept proposed by American writer Joseph Campbell. In addition, the chapter also presents examples of sources of inspiration for storytelling. Among them, it is worth mentioning, for example: history of the company’s founder or president, the beginnings of the company, employees’ stories or milestones (successes or failures). The last part presents two case studies of the use of storytelling by enterprises. These are examples from the Polish market regarding the use of storytelling in employee recruitment and customer engagement.
Los movimientos eclesiales y las nuevas comunidades: entre institución y carisma
Authors: Delgado Galindo Miguel
Published: December 2021

Los movimientos eclesiales y las nuevas comunidades son realidades eclesiales portadoras de carismas que el Espíritu Santo distribuye para el bien común de la Iglesia, con el fin de contribuir a la santidad no solo de un conjunto determinado de fieles, sino de todos los miembros del Pueblo de Dios. Los movimientos eclesiales y las nuevas comunidades, integrados mayoritariamente por fieles laicos, encuentran su origen en la eclesiología de comunión que caracteriza el magisterio del Concilio Vaticano II, y constituyen un motivo de esperanza para la evangelización en este periodo de la historia. La profundización de la eclesiología del último concilio ecuménico ha puesto de manifiesto la coesencialidad entre los dones jerárquicos y carismáticos en la constitución divina de la Iglesia, aspecto que ha sido elevado a la categoría de principio teológico. Los últimos papas han expresado su estima hacia estas nuevas realidades eclesiales, han establecido criterios para su discernimiento, han dado indicaciones generales para facilitar su armónica inserción en las Iglesias particulares donde se encuentran presentes sus miembros y han contribuido al proceso de conversión misionera en la que está comprometida la Iglesia entera.
Some Reflections Regarding the Public Pension and The Number of Pensioners in Romania, in The Period 2010-2021
Authors: Bălăneasa Maria-Cristina , Dogotari Cătălina
Published: December 2021

The current importance of public pensions is given by the fact that this is the main form of support for inactive or unemployed people.Through this article we aim to review, in a brief way, the evolution and particularities of public pension.In particular, we want to analyze the evolution of the number of retirees in the public system, of the average pension but also of the service pensions during the years 2010-2021, in order to identify some directions for improving the public pension system.
Alterations in the Hemato-biochemical, Endocrine, Somatic Cell Count and Milk Composition in Lactating Tarai Buffaloes during Different Lactation Stages
Authors: Huozha Ruokuobeinuo , Mukherjee Joydip , Begum Rizwana , Rastogi Sunil Kumar
Published: December 2021

Background: Tarai buffalo is indigenous buffalo breed of Uttarakhand state, dual purpose, well adapt to hot-humid climatic condition of Tarai area. This study aimed to evaluate the alterations in the hemato-biochemical, endocrine, milk somatic cell count and milk composition in lactating Tarai buffaloes. Methods: Thirty-six healthy Tarai buffaloes were selected from four different Gujjar farms and divided into four groups consisting nine buffaloes in each group as early (60±30 days), mid (120±30 days) and late (180±30 days) lactation stages and dry buffaloes. Both blood and milk samples were collected once from each lactation stage of selected animals. Hematological parameters and milk composition were evaluated by standard procedures and methods. Biochemical and endocrine parameters were evaluated using commercially available kits as per manufacturer’s protocol. Milk somatic cell count (SCC) was done by direct microscopic counts using Newman Lampert stain. Result: It observed significantly (p less than 0.05) higher TLC value but lower plasma glucose, cholesterol, calcium, phosphorus and urea levels during early lactation stage. Significantly (p less than 0.05) declined in plasma total protein during late lactation stage and triglyceride in dry cows. Plasma hormone significantly (p less than 0.05) higher in cortisol during early lactation while higher T4 and insulin in late lactation stages. Milk fat, urea and total solids were significantly (p less than 0.05) decreased during early lactation and again increased during late lactation while milk SCC significantly (p less than 0.05) higher during early and late lactation stages.
Hubungan Motivasi Berprestasi dengan Stres pada Siswa di SMA Negeri 1 Jejawi
Authors: Rahman Rani , Alimron Alimron , Fauzi Muhammad
Published: December 2021

Penelitian ini bertujuan untuk mengetahui hubungan antara motivasi berprestasi dengan stres di SMA Negeri 1 Jejawi. Metode penelitian yang digunakan adalah kuantitatif deskriptif dengan pendekatan kuantitatif. Sampel yang digunakan sebanyak 30 siswa dari 100 siswa. Penelitian ini menyimpulkan bahwa tidak ada hubungan yang signifikan antara motivasi berprestasi dengan stres.
Mikrogravitenin Elektroensafalogram Bulguları Üzerine Etkisi: Bir Derleme Çalışması
Authors: SARITAŞ Serdar , TARLACI Sultan
Published: December 2021

Tüm diğer canlılar gibi insanlar da yeryüzündeki fizik kanunları çerçevesinde doğar ve yaşarlar. Ancak bu durum 20 yy. ortasından itibaren değişmiş ve insanlar, uzay yolculuklarına başlayarak, bu kuralların dışında yaşamayı tecrübe etmeye başlamıştır. Uzay, düşük yerçekimi (mikrogravite) ve kozmik radyasyon gibi zorlukları olduğu gibi, uzay araçları (Uluslararası uzay istasyonu-ISS da dahil) da kısıtlı bir alana mahkum olma, izolasyon, aile ve sosyal çevreden ayrı kalma gibi güçlükleri de beraberinde getirmektedir. Tüm bu zorluklar, insan fizyolojisi ve psikolojisine de etki etmektedir. Özellikle sinirbilim alanında bu sorunları, denge (vestibuler sistem), uzay oryantasyonu, görme, propriyosepsiyon, motor kontol ve uyku konusunda görmek mümkündür. Bu problemlerin anlaşılması ve tedavisi için yerdeki kontrol merkezi ile daima iletişim kurulur. Ayrıca uzay aracı içinde bulunan tanı araçlarından da yararlanılır. Taşınabilir olması ve kullanımı kolay olduğu için en sık kullanılan yöntemlerden birisi elektroensafalogramdır (EEG). Şu ana kadar daha çok yer işgal eden ve ağır bir cihaz olan MRG (Manyetik Rezonans Görüntüleme) ve CT (Bilgisayarlı Tomografi) uzaya taşınamamış, sadece astronotların uzay uçuş öncesi ve sonrası beyin görüntüleri alınabilmiştir. Bu derleme yazısı sınırlı literatür bilgisi ışığında astronotların uzay uçuşu sırasındaki EEG değişikliklerini kısmen de olsa açıklayabilmek üzere hazırlanmıştır.
Chatbot as a Telehealth Intervention Strategy in the COVID-19 Pandemic
Authors: Azevedo Chagas Bruno , Ferreguetti Kícila , C. Ferreira Thiago , S. Marcolino Milena , B. Ribeiro Leonardo , S. Pagano Adriana , S. N. Reis Zilma , O. Prates Raquel , Meira Jr. Wagner , L. Ribeiro Antonio
Published: December 2021

The COVID-19 pandemic and the need for social distancing have created a demand for new and innovative solutions in healthcare systems worldwide. One of the strategies that have been implemented are chatbots, which can be helpful in providing reliable health information and preventing people from seeking assistance in healthcare centers and being unnecessarily exposed to the virus. In this context, although a high number of chatbots have been implemented worldwide, little has been discussed about the process and challenges in developing and implementing this technology. This paper reports on an action research, which designed a novel chatbot as a prompt response to the COVID-19 pandemic. The chatbot is intended to be a first layer of interaction with the public, performing triage of patients and providing information about COVID-19 on a large scale and without human contact. Our contribution is twofold: (i) we reflected on the development process and discuss lessons learned and recommendations to support a multidisciplinary development and evolution process of the chatbot; and (ii) we identified some interactive and technological features that can be used as a reference framework for this kind of technology. These contributions can be useful to other researchers and multidisciplinary teams facing similar challenges.
The current state and prospects for hydrogenisation of motor transport in Northwestern Europe and Poland
Authors: Gis Maciej , Gis Wojciech
Published: December 2021

The article presents the situation regarding the hydrogenisation of motor transport in Northwestern Europe, a region leading in this regard in Europe. The following countries were included in the analysis of national plans in this area, taking into account both technical issues – among others - concerning HRS and FCEV, their number, as well as economic issues (among other things relating to the costs of using hydrogen): Belgium, Denmark, France, Germany, the Netherlands, Norway and England. Reference was also made to the situation in Poland, where major fuel and energy companies (among others: Orlen, Lotos, PGNiG and ZE PAK Capital Group) are strongly interested in hydrogenisation of motor transport and manufacturers of vehicles – for example – Solaris or Autosan in producing vehicles equipped with fuel cells. Based on the analyses carried out at the Institute of Motor Transport, it was found that the good location of basic hydrogen refuelling stations is along the TEN-T corridors running across Poland. The order of their location is as follows: 1- Poznan,2-Warsaw,3 Bialystok, 4-Szczecin, 5- Łódź region, 6-Tricity, 7-Wroclaw, 8- Katowice region, 9-Kraków.
Characterisation of Phenolic Compounds, Sterols and Geographical Fingerprint of Çekişte Extra-Virgin Olive Oils According to their Geographical Locations by Using LC IMS QTOF Mass Spectrometry
Authors: Uckun Ayca Akca
Published: December 2021

In this context, aim of this study is to determine the effect of Çekişte olive oils in different locations and show differences on geographical locations while taking geographical indication label. Çekişte olive oil variety which cultivated in six different locations (Birgi, Bademli, Beyazit, Yeniceköy, Zeytinlik, Uzumlu) were evaluated the effects of geographical locations on the chemical characterization of in the southwest of Turkey. The agricultural ecological map of each location was created using GIS. Olive oil samples were analyzed fatty acid, sterol and phenolic. Moreover, LC IMS Qtof spectrometer and Progenesis QI software were used to determine the geographical fingerprints of olive oil samples in different locations. Results showed that oil qualities of some locations differ significantly depending on olive growing area (p <0.05), some of them not. The Principal Component Analysis of the different locations analyzed revealed that "geographical location" factor significantly affects the olive oil quality.
PENGELOMPOKAN KABUPATEN/KOTA DI PROVINSI JAWA TENGAH BERDASARKAN JUMLAH PEREMPUAN (USIA 18+) KORBAN KEKERASAN DENGAN MENGGUNAKAN K-MEANS CLUSTERING
Authors: Yulianti Evi , Sugandha Agus
Published: December 2021

Violence against women is one of the cases that often gets attention and news in various mass media and electronic media today. In Komnas Perempuan's Annual Records, there were 406,178 cases of violence against women reported and handled throughout 2018. This number increased compared to the previous year's 348,466 cases. This study was conducted to try to group 35 districts/cities in Central Java into 3 clusters based on the number of women (age 18+) victims of violence. Grouping is done using the k-means cluster method. The regencies/cities in Central Java are grouped into 3 clusters, namely: cluster 1 there is 1 Regency/City with a high number of women (age 18+) victims of violence, cluster 2 there are 4 Regency/City with a high number of victims of violence. female (age 18+) victims of violence. moderate, and cluster 3 there are 30 districts/cities with a low number of women (age 18+) victims of violence.
Enjoying an Empty Edifice
Authors: Blanuša Nebojša , Jerbić Vedran
Published: December 2021

This paper reaffirms the methodological potentials of Lacanian psychoanalysis for the theories of nationalism. From the Lacanian perspective, national consciousness and self-determination are only possible in the fantasmatic frame­work through the (mis)recognition and retroactive construction. National imagination is the form of transference, necessary for performing the nation through invented traditions and rituals. However, beyond symbolization and imaginary (mis)recognition, there is always something that resists closure, linked with the subjects' desire and organized around the lack of the subjects' full enjoyment. Taking together all these aspects, we build an analytical framework for the study of nationalism, which comprises a quadruple system of identifications by referring to the concepts of Ideal-Ego, Ego-Ideal, Super-Ego and specular Other, and illustrate it through the example of the AKP's Turkish nationalism.
The Three Versions of the Ultimatum Game
Authors: Pavlović Dušan , Đurašković Stevo
Published: December 2021

We examine the 1914-1918 creation of the Kingdom of Serbs, Croats and Slovenes as a form of the ultimatum game. The negotiations among the Serbian Cabinet and the yugoslav Committee representatives of the Habsburg Souths Slavs from 1914-1918 exemplify three versions of this game. The first version is a typical (rational choice) type of the ultimatum game in which the receiver is satisfied with any offer by the Proposer. The second version is an instance of behavioral game theory. When the Proposer gives an unfair offer, it provokes an emotional reaction in the receiver who will reject it at the cost of harming themselves. We observe this behavior in the emotional behavior of frano Supilo, a Croat and one of the leaders of the yugoslav Committee. The third version of the behavioral ultimatum game can be observed in the behavior of Serbian Prime minister nikola Pašić who opposed any concessions to the yugoslav Committee, thus giving an ultimatum to the Croat side to accept the Serbian offer or remain with nothing, which was harmful to the Serbian side, too. This example is important because it produces two conclusions. first, historical games are often a mixture of several versions. Second, Proposers, too, can have an emotional reaction and give an offer that can hurt themselves. This aspect of the ultimatum game is less mentioned because it is difficult to simulate in experiments.
Interpretación de la curación periapical en imágenes radiológicas. Una revisión
Authors: Archila-Montañez Eloísa , Medina-Ocampo Paola Elena
Published: December 2021

La interpretación mediante imágenes radiológicas del estado de curación de una periodontitis apical crónica o de un curetaje apical con apicectomía, se basa en el análisis de los cambios en el aspecto del área periapical, en la estructura del hueso adyacente al sitio de la inflamación o de la intervención quirúrgica, que se observan proyectados sobre estructuras óseas normales como las corticales. Por esto, al interpretar radiografías, se deben considerar limitaciones propias como la distorsión de estructuras o la superposición de imágenes anatómicas. En los estudios tradicionales de evaluación del área periapical después de un tratamiento, apoyados en imágenes 2D, los casos considerados exitosos o los fallidos se han comparado con un estándar de referencia, como el índice periapical o los criterios de evaluación del resultado del tratamiento quirúrgico endodóntico. Actualmente, algunos autores han referido otras condiciones que podrían intervenir en la evaluación, por ejemplo, que la apariencia radiográfica de la corona y la raíz del diente a evaluar pueden influir en la interpretación del área periapical. Adicionalmente, la tomografía computarizada de haz cónico (TCHC) permite relacionar el tejido lesionado o en proceso de reparación con las tablas vestibular y lingual para medir su periferia en una reconstrucción transversal. En efecto, durante el proceso de curación de los tejidos periapicales y radiculares, se presentan eventos biológicos complejos y mecanismos que pueden ocurrir a un mismo o en diferente tiempo. El conocimiento de la diversidad de tejidos es fundamental para identificar la dinámica de los signos de regeneración o si se produjo la curación por reparación. Por tales razones, el propósito de este artículo fue identificar en la literatura actual los criterios utilizados para interpretar la curación periapical en imágenes bidimensionales o tridimensionales, y su relación con el diagnóstico y tratamiento.
Beliefs and Perceptions About Parenteral Nutrition and Hydration By Advanced Cancer Patients
Authors: Abe Akiko , Amano Koji , Morita Tatsuya , Miura Tomofumi , Mori Naoharu , Tatara Ryohei , Kessoku Takaomi , Matsuda Yoshinobu , Tagami Keita , Otani Hiroyuki , Mori Masanori , Taniyama Tomohiko , Nakajima Nobuhisa , Nakanishi Erika , Kako Jun , Kiuchi Daisuke , Ishiki Hiroto , Matsuoka Hiromichi , Satomi Eriko , Miyashita Mitsunori
Published: December 2021

PurposeObjectives of this study are to clarify the beliefs and perceptions of parenteral nutrition and hydration (PNH) by patients with advanced cancer and to examine the relationships between their beliefs and perceptions and cachexia stages.MethodsWe conducted a questionnaire survey. We asked about patient characteristics and anthropometric measurements. We subsequently asked patients to answer 15 items regarding their beliefs and perceptions. The proportions of patients were calculated. Comparisons were performed using the Mann-Whitney U test. We conducted an explanatory factor analysis and calculated Cronbach’s alpha coefficients to assess the internal consistency. A multiple logistic regression analysis was performed to identify the independent factors affecting cancer cachexia stages.ResultsAmong 495 patients, 378 responded. Due to missing data, 357 remained in the frequency distribution analysis and explanatory factor analysis, and 344 were classified into the non-cachexia group (n = 174) and cachexia group (n = 170). Approximately 60% of patients thought that PNH were beneficial. Approximately 70% considered PNH as a standard medical practice. Approximately 70% did not feel that they received a sufficient explanation. There were no significant differences in any items between the two groups. We extracted four conceptual groups and Cronbach’s alpha coefficients were 0.87, 0.73, 0.71, and 0.48, respectively. The concept of ‘Belief that PNH are harmful’ was identified as an independent factor [odds ratio 2.57 (95% confidence interval 1.10-6.01), P = 0.030].ConclusionsThis study revealed that patients had a strong preference for receiving PNH with or without cancer cachexia.
The Feasibility of Edoxaban for Asymptomatic Cancer-associated Thrombosis in Japanese Gastrointestinal Cancer Patients (ExCAVE study)
Authors: Nakamura Michio , Ishiguro Atsushi , Dazai Masayoshi , Kawamoto Yasuyuki , Yuki Satoshi , Sogabe Susumu , Hosokawa Ayumu , Sawada Kentaro , Muto Osamu , Izawa Naoki , Nakashima Koji , Horie Yoshiki , Yagisawa Masataka , Kajiura Shinya , Ando Takayuki , Mitsuhashi Yosuke , Sunakawa Yu , Kikuchi Yasuka , Komatsu Yoshito
Published: December 2021

BackgroundAlthough an initial therapy with a parenteral anticoagulant is required before edoxaban, this strategy is frequently avoided in actual clinical practice because of its complexity. This study assessed the feasibility of edoxaban without initial heparin usage for asymptomatic cancer-associated thrombosis (CAT) in Japanese patients with gastrointestinal cancer (GIC) at a high risk of bleeding.MethodsIn this multicenter prospective feasibility study conducted at 10 Japanese institutions, patients with active GIC who developed accidental asymptomatic CAT during chemotherapy were recruited. Edoxaban was orally administered once daily without initial parenteral anticoagulant therapy within 3 days after detecting asymptomatic CAT. The primary outcome was the incidence of major bleeding (MB) or clinically relevant non-major bleeding (CRNMB) during the first 3 months of edoxaban administration. ResultsOf the 54 patients enrolled from October 2017 to September 2020, one was excluded because of a misdiagnosis of CAT. In the remaining 53 patients, the primary outcome occurred in six patients (11.3%). MB occurred in four patients (7.5%), including gastrointestinal bleeding in three patients and intracranial hemorrhage in one patient. CRNMB occurred in two patients (3.8%), including bleeding from the stoma site and genital bleeding in one patient each. There were no deaths attributable to bleeding, and all patients who had experienced MB and CRNMB recovered.ConclusionsThe risk of bleeding after edoxaban without heparin pretreatment was acceptable, demonstrating new treatment options for asymptomatic CAT in patients with GIC.
Estimation of the Present Status of the Species Based on the Theoretical Bounds of Environmental Noise Intensity: An Illustration Through a Big Abundance Data and Simulation
Authors: Paul Ayan , Ghosh Nabakumar , Bhattacharya Sabyasachi
Published: December 2021

Sibly et al. (2005) described that most species have a fundamental characteristic to follow the theta-logistic growth trait with the convex downward trend. The fundamental yardstick of this research work builds under the deterministic setup, whereas the involvement of the external noise in any growth system is inevitable. But, the involvement of external affairs in any species growth can't be well judged only through its density dependence; it requires a further assessment. So, we frame the theta-logistic model with the stochastic analog in two directions, i.e., the discrete and continuous setup. The analysis of the discrete stochastic model is manifested by the bifurcation analysis, which shows that the attainment of the chaotic regime enhances with the increase in noise intensity level. Although the role of chaos in species extinction is debatable, a literature survey suggests that chaos with stochasticity accelerates the extinction of species. Similarly, in the case of the continuous version, we performed a theoretical study on the stochastic theta-logistic model to provide a critical value of the noise intensity parameter. This threshold magnitude act as the sustainability criteria of any species environmental tolerability. In this connection, we use the data of four major taxonomic groups, i.e., Bird, Insect, Mammal, and Fish, from the GPDD database and classify the species based on environmental sensitivity. The high sensitive species have a low tolerance level, associated with the small magnitude of environmental noise intensity parameter. Moreover, the simulation prediction model on these four taxonomic classes also shows that the overall extinction probability of the Bird is almost negligible for the current time window.
The Critical Role of Polyketide Synthase Gene On The Swainsonine Biosynthesis in The Fungus Metarhizium Anisopliae
Authors: Sun Lu , Huang Enxia , Zhang Yu , Guo Ziyu , Wu Kexin , Zhang Yunhao , Mo Chonghui , Wang Jinglong , Zhao Baoyu , Lu Hao
Published: December 2021

Swainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by fungi including Metarhizium anisopliae, Slafractonia leguminicola, and Alternaria oxytropis. While the SW biosynthesis pathway of fungi and the catalytic enzyme genes that regulate synthesis are not cleanly. In this study, we used homologous recombination (HR) to knock out and interfere with the polyketide synthase gene (pks) of M. anisopliae to determine its effect on the SW biosynthesis pathway. The concentration of SW was measured in the fermentation broth of M. anisopliae at 1 d, 2 d, 3 d, 4 d, 5 d, 6 d or 7 d using LC-MS. The gene for the pks gene was detected by RT-qPCR. Day 5 of M. anisopliae gave the highest content of SW and the highest expression of the pks gene. To determine the role of the pks gene in the SW biosynthesis pathway of M. anisopliae, we used PEG-mediated homologous recombination (HR) to transform a wild-type strain (WT) with a Benomyl (ben)-resistant fragment to knock out the pks gene producing a mutant-type strain (MT) and used PEG-mediated RNAi to transform a wild-type strain (WT) with a Benomyl (ben)-resistant plasmid to interfere with the pks gene. A complemented-type (CT) strain was produced by adding a complementation vector that contains the geneticin (G418) resistance gene as a marker. The content of SW didn’t detected in MT strain, and returned to the original level in the CT strain, while the content of SW was significantly decreased in RNAi strain. We suggest that mutation and RNAi in the pks gene affect the cell wall formation of M. anisopliae, while the colony diameters, phenotypes, and growth rates did not change significantly, and no obvious changes in other cellular organelles were noted. These results indicate that the pks gene plays a crucial role in the SW biosynthesis of M. anisopliae, which provides an important theoretical basis for illuminating the SW biosynthesis and solving locoism in livestock.
Non-Wilms' Renal Tumors In Children:A 139 Cases Series
Authors: Fang Yi Wei , Song Hong Cheng , Sun Ning , Zhang Wei Ping
Published: December 2021

Background:Pediatric non-Wilms renal tumors (NWRTs) which comprise a small proportion of renal tumors,are a heterogeneous group of neoplasms with variable malignant potential, mortality, and response to treatment.This study aimed to determine the clinical characteristics, management and prognosis of children with non-Wilms' renal tumors(NWRTs). Methods:Medical records of all patients (n = 139) treated for NWRTs over a 12-year period (2008.01–2019.10) at a single center were reviewed retrospectively.Results:The histopathological groups of NWRTs included malignant rhabdoid tumor of the kidney(MRTK)(n: 30, 21.6%), renal cell cancer(RCC)(n: 26,18.7%), clear cell sarcoma of the kidney(CCSK)(n: 24,17.3%), congenital mesoblastic nephroma(CMN)(n: 21,15.1%), cystic nephroma(CN)(n: 16,11.5%),metanephric tumours(n: 12, 8.6%), renal angiomyoliporma(RAML)(n: 3, 2.2%), renal primitive neuroectodermal tumor(rPNET)(n: 2, 1.4%), renal hemangioma(n: 2, 1.4%), inflammatory myofibroblastic tumor(IMT)(n: 2, 1.4%), ossifying renal tumor of infancy(ORTI)(n: 1, 0.7%). 123 children were followed up with an average of 42 months. 16 children were lost to follow-up. Tumor-free survival was observed in 94 children. 28 children(22.8%) were died.Conclusions:Pediatric NWRTs comprises 19.1% of all renal tumors in our single center. Accurate diagnoses along with appropriate management are important factors in improving patients outcome. The mainstay treatment of malignant NWRTs including MRTK,CCSK,RCC and PNET is comprehensive treatment. The mainstay treatment of benign NWRTs including RAML,CN, ORTI, CMN,metanephric tumours, and renal hemangioma is surgical resection alone.
Food Consumption Patterns, Breakfast, Meals with Family and Associations with Common Mental Disorders in Adolescents: A School-Based Cross-Sectional Study
Authors: Gratão Lucia Helena Almeida , Pessoa Milene Cristine , Silva Thales Philipe Rodrigues da , Rocha Luana Lara , Inácio Monique Louise Cassimiro , de Oliveira Tatiana Resende Prado Rangel , Cunha Cristiane de Freitas , Mendes Larissa Loures
Published: December 2021

BackgroundAdolescence is a period of transition and vulnerabilities, in which mental illnesses can develop more easily. The objective of this work is to verify the association between food consumption patterns and the presence of Common Mental Disorders in Brazilian adolescents. MethodsThis is a cross-sectional study which analyzed data from 71,553 Brazilian adolescents aged 12-17 years, from the ERICA Study. Principal Component Analysis was performed to identify Food Consumption Patterns, and Logistic Regression Models were performed to identify the associations between Common Mental Disorders, Food Consumption Patterns, and eating practices that are breakfast consumption and practice of having meals accompanied by family. ResultsTwo patterns were found, a Healthy Food Consumption Pattern and a Unhealthy Food Consumption Pattern. Adolescents classified in the second (OR: 0.77; 95% CI 0.68 - 0.88) or third (OR: 0.87; 95% CI 0.74 - 0.95) tercile of the Healthy Food Consumption Pattern had a lower chance of having Common Mental Disorders, while those who belonged to the third tercile of the Unhealthy Food Consumption Pattern (OR: 1.22; 95% CI 1.02 - 1.42), had an increased chance of having Common Mental Disorders. Eating breakfast sometimes (OR: 0.69; 95% CI 0.59 - 0.80) or almost every day/every day (OR: 0.52; 95% CI 0.46 - 0.59), and the practice of having the main meals with the family sometimes (OR: 0.64; 95% CI 0.54 - 0.75) or almost every day/every day (OR: 0.48; 95% CI 0.40-0.55), decreased the chance for Common Mental Disorders.ConclusionStrategies to promote the mental health of adolescents as the adoption of healthy eating practices must be strengthened and promoted.
Spontaneous Regression of Tumor in an Optic Pathway Glioma Patient With Diencephalic Syndrome: Case Report and Literature Review
Authors: Luo Rutao , Zhang Tianlei , Yang Wei , Ji Yuanqi , Sun Hailang , Li Dapeng , Ge Ming
Published: December 2021

Background: Diencephalic syndrome (DS) can cause failure to thrive in pediatrics, which is mostly found in optic pathway gliomas (OPGs) patients. OPGs patients with DS always show a poor outcome. Case presentation: We present the case of an OPG patient with DS who got a spontaneous regression without any treatment. A 6-month-old girl presented with failure to thrive for two months and visual dysfunction with bilateral horizontal nystagmus. MRI demonstrated a 42mm*37mm*36mm enhanced and lobulated lesion located in the sellar region with a clear boundary with surrounding tissues. OPG and DS was diagnosed according to her radiological examination and manifestation. Conservative follow-up was given. There was a spontaneous decrease in tumor size during follow-up. Symptom improves and the patient continues to have a good quality of life despite a moderate dysfunction of her left eye.Conclusions: Conservative observation can be used as a treatment for some OPG patients, body weight may be a marker of the growth of tumor in OPG patients with DS.
Composition And Key-Influencing Factors of Bacterial Communities Active In Sulfur Cycling of Soda Lake Sediments
Authors: Li Xiangyuan , Yang Maohua , Mu Tingzhen , Miao Delu , Liu Jinlong , Xing Jianmin
Published: December 2021

Bacteria are important participants in sulfur cycle of the extremely haloalkaline environment, e.g. soda lakes. The effects of physicochemical factors on the composition of sulfide-oxidizing bacteria (SOB) and sulfate-reducing bacteria (SRB) in soda lakes have remained elusive. Here, we surveyed the communities structure of total bacteria, SOB and SRB based on 16S rRNA, soxB and dsrB gene sequencing, respectively, in five soda lakes with different physicochemical factors. The results showed that the dominant bacteria in soda lakes sediments belonged to the phyla Proteobacteria, Bacteroidetes, Halanaerobiaeota, Firmicutes and Actinobacteria. SOB and SRB were widely distributed in lakes with different physicochemical characteristics，and the community composition were different . In general, salinity and inorganic nitrogen sources (NH4+-N, NO3--N) were the most significant factors. Specifically, the communities of SOB, mainly including Thioalkalivibrio, Burkholderia, Paracoccus, Bradyrhizobium, and Hydrogenophaga genera, were remarkably influenced by the levels of NH4+-N and salinity. Yet, for SRB communities, including Desulfurivibrio, Candidatus Electrothrix, Desulfonatronospira, Desulfonatronum, Desulfonatronovibrio, Desulfonatronobacter and so on, the most significant determinants were salinity and NO3--N. Besides, Rhodoplanes played a significant role in the interaction between SOB and SRB. From our results, the knowledge regarding the community structures of SOB and SRB in extremely haloalkaline environment was extended.
Together Apart: Increased Crowd Heterogeneity Influences Crowd Dynamics and Interpersonal Distance at Bottlenecks
Authors: Geoerg Paul , Schumann Jette , Boltes Maik , Kinateder Max
Published: December 2021

Despite considerable research efforts, most controlled empirical studies on crowd movement usually rely on homogeneous crowds, i.e., research participants are typically young adults without disabilities. Consequently, little is known about pedestrian movement in more diverse and heterogeneous crowd conditions, e.g., when persons with reduced mobility are present. This gap may be particularly relevant at bottlenecks, along the path of a moving crowd that limit the capacity of pedestrian flow. Here, we present results from 12 studies in which participants (total N = 252) with and without visible disabilities moved together in a crowd. In each study, groups of participants walked together in a hallway with a bottleneck at the end. The point of speed adoption, distances between neighbours, and behavioural activities were analysed. We found (1) that participants with disabilities reduced their speed further away from the bottleneck than participants without disabilities; (2) participants without disabilities stayed closer to neighbors with disabilities than to neighbors without disabilities; and (3) participants interacted and communicated with each other to organise in front of the bottleneck. These results underline the importance of studying representative and heterogeneous samples in crowd dynamics. We also argue that more interdisciplinary research is needed to better understand the dynamics of interactions between neighbors in a crowd. A more nuanced understanding of pedestrian dynamics holds the promise of improving the validity of simulation tools such as movement and evacuation models.
GPER Activation Attenuates Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Sex-Dependently and Inhibits Alveolar Macrophage Activation
Authors: Yang Cui , Cong Zhukai , Zhao Feng , Shen Ziyuan , Zhu Xi
Published: December 2021

Background: Acute respiratory distress syndrome (ARDS), a common and critical disease, is clinically characterized by uncontrolled inflammation and alveolar-capillary barrier disruption. Estrogen can reportedly alleviate ARDS caused by numerous insults in mice. Moreover, the estradiol receptors α, not β,participated in E2-induced attenuation of ARDS. But the role of another estradiol receptor, G protein-coupled estradiol receptor 1 (GPER1) in ARDS are not undertood. This study is aimed to investigate the effect of GPER activation on LPS-induced ARDS in mice.Methods: Female mice were randomly subjected to bilateral ovarectomy (OVX) or sham surgery two weeks before lung injury. The GPER-selective agonist G1 or vehicle were intraperitoneally injected 0.5 h before intratracheal administration of LPS or phosphate-buffered saline in male and female mice. After 24 h, mice were sacrificed to collect blood, bronchoalveolar lavage fluid (BALF), and lung tissue. Histological injury and inflammatory cell infiltration in lung tissue, as well as cytokine and protein concentrations in BALF were determined. In vitro experiments were also performed on alveolar macrophages (MH-S cells) to investigate the effect of GPER activation on LPS-induced inflammatory responses.Results: Activation of GPER by G1 administration significantly ameliorated lung pathological damage, attenuated alveolar capillary barrier destruction, inhibited recruitment of inflammatory cells into alveoli, and decreased concentrations of the pro-inflammatory factors tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in BALF of LPS-administered male and OVX female mice, but not intact female mice. In vitro experiments demonstrated that G1 pretreatment significantly inhibited LPS-mediated increases of TNF-α, IL-6, and MIP2 in a dose-dependent manner.Conclusions: These results demonstrated that GPER activation attenuated lung injury of male and OVX female mice by inhibiting the inflammatory response of alveolar macrophages.
Decomposition of Fine Roots and Aboveground Agroforestry Litter as Plant-soil Feedback After Volcanic Ash Deposition
Authors: Purnamasari Eka , Noordwijk Meine van , Maulana Rizky , Saputra Danny , Sari Rika Ratna , Hairiah Kurniatun
Published: December 2021

Background and PurposeAbove- and belowground organic inputs feed decomposer communities in the soil enhancing soil organic matter (Corg) formation, depending on the vegetation, soil, contextual factors and human management of (agro)ecosystems. Plant-soil feedback in volcanic ash rapidly increases Corg during transformation to Andisols. We quantified fine root turnover in agroforestry systems, including the ash-adapted native tree Parasponia rigida, as part of the C accumulation process. MethodsFine root (<2 mm) decomposition was quantified with a total of 1440 litterbag samples, testing the effects of six tree species (Coffea canephora, Persea americana, Durio zibethinus, Gliricidia sepium, Falcataria moluccana and Parasponia rigida), three distances to the nearest coffee trees, two seasons (rainy and dry), two sites (with and without recent ash deposits), four time intervals (2, 4, 6 and 8 weeks) and five replicates. Soil temperature around the litterbags was used to derive equivalent decomposition rates at 20oC. The ratio of lignin plus phenolics over nitrogen was used as main litter quality indicator.ResultsDecomposition of fine tree roots was up to three times faster than that of aboveground litter with the same quality index measured in the same habitat. Root decomposition was slower in topsoils with recent volcanic ash, with a mean residence time extended by, on average, two weeks. Decomposition of roots of the ash-adapted native tree Parasponia rigida was especially rapid. ConclusionsFine root turnover contributes to the Corg accumulation that turns low-C volcanic ash into high-carbon andic soil and has relatively short necromass residence times.
Association of Circulating Circular RNAs (mmu-015947, hg38-0008980, and DLGAP4) in Diagnosis, Diseases Severity, and Prognosis of Ischemic Stroke
Authors: Zeraatiannejad Mastaneh , Bayat Mahnaz , Tabrizi Reza , Borhani-Haghighi Afshin , Mokhtari Mohammad Javad
Published: December 2021

Backgrounds Quickly diagnosing ischemic stroke (IS) is a critical issue in clinical studies, as it allows more effective therapy and stops the progression of IS. The blood level of CircRNAs after stroke may act as a rapid diagnostic marker.Methods and Results In this study, the blood level of circRNAs was evaluated using a real-time PCR. We used logistic and linear regression analysis to evaluate the potential of circRNAs levels with the risk of IS. Circ-mmu-015947 was up-regulated in patients. Its expression also showed a good value in predicting IS risk, and a significant diagnostic value. CircDLGAP4 was decreased in patients compared with controls and logistic regression showed its expression negatively associated with IS risk. The expression level of hg38 was reduced significantly in patients with small vessel disease (SVD) and the linear regression analysis showed a negative relationship between hg38 expression with SVD subtype. Circhg38 expression relative to controls showed a significant association with IS risk. Conclusion Taken together, circulating circ-mmu-015947 may serve as a novel biomarker for IS, and we found a significant decrease in the level of hg38-0008980 after IS it may act as a novel circRNA in IS pathophysiology with a positive correlation with stroke severity.
Roots With Larger Specific Root Length and C: N Ratio Sustain More Complex Nematode Community
Authors: Zhang Jingru , Hu Zhengkun , Zhang Chongzhe , Tao Yiheng , Chen Xiaoyun , Griffiths Bryan , Liu Manqiang
Published: December 2021

Aims Roots bridge above and belowground systems, and play a pivotal role in structuring root-associated organisms via influencing food resources and habitat conditions. Most studies focused on the relationships between plant identity and root-associated organisms, however, little is known about how root traits affect nematode communities within the rhizosphere. Methods We investigated the relationships between root traits of four plant species and nematode diversity, community structure and trophic complexity in an ex-arable field. Results While the relative abundance of herbivorous nematodes was negatively associated with specific root length (SRL), specific root area (SRA), root length density (RLD) and root C: N ratio, free-living nematodes were positively affected by these traits, implying a multifaceted effect of root traits on root-associated organisms. Importantly, we found that finer root systems promoted the complexity of the nematode community, by increasing the relative abundance of high trophic-level nematodes (i.e., omnivores and predators) and enhancing nematode diversity. Conclusion Our findings suggest that root traits could be reliable indicators of soil community structure and interactions, and provide new insights into soil biodiversity and functional maintenance.
Removal of Silica in Wastes With Methyl Cellulose Composite Membranes
Authors: Dağıdır Demet , Acaralı Nil
Published: December 2021

In this study, experiments were carried out for composite membrane synthesis with m-phenylenediamine (MPD), hydroxyethyl methacrylate (HEMA) and methyl cellulose (MS) by changing process parameters (methyl cellulose ratio, synthesis temperature, stirring time, oven temperature). Taguchi Method, an optimization method for production, was applied to 4 levels 4 parameters. The silica solution was passed through the produced composite membrane, and the filtering property of the membrane for different periods was examined. Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) were used for characterization of composite membranes. Shore A test was performed to determine the mechanical strength of composite membranes. The amount of silica in membranes obtained because of the silica treatment was determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysis. As a result, it was seen that the composite membranes produced could be evaluated in the treatment of wastewater containing silica.
Targeting the Tumor Immune Microenvironment Could Become a Potential Therapeutic Modality for Invasive Pituitary Adenoma.
Authors: Yang Zuocheng , Tian Xuemei , Wang Chen , Yao Kun , Yang Yakun , Zhang Linpeng , Liu Ning , Yan Changxiang , Qi Xueling , Han Song
Published: December 2021

Objective: The study aimed to explore the relationship between the invasiveness and immune cell infiltration in pituitary adenoma (PA) and provide the basis for immuno-targeting therapies.Methods: One hundred three patients who underwent surgery at a single institution were retrospectively identified. The infiltration of macrophages and T lymphocytes was quantitatively assessed in PA.Results: The number of CD68+ macrophages was positively correlated with Knosp (P=0.003) and MMP-9 grades (P=0.00). The infiltration of CD163+ macrophages differed among Knosp (P=0.022) and MMP-9 grades (P=0.04). CD8+ tumor-infiltrating lymphocytes (TILs) were also positively associated with Knosp (P=0.002) and MMP-9 grades (P=0.01). Interestingly, MGMT expression was positively correlated with MMP-9 staining extent (P=0.000). The quantity of CD8+ TILs (P= 0.016), CD68+ macrophages (P=0.000) and CD163+ macrophages (P=0.043) were negatively associated with MGMT expression levels. The number of CD68+ macrophages in the PD-L1 negative group was significantly more than in the PD-L1 positive group (P=0.01). The rate of PD-L1 positivity had positive correlations with the Ki-67 index (P=0.046) and p53 expression (P =0.029). Conclusion: Targeted therapy for macrophages and CD8+ TILs could be a helpful treatment in the future for invasive PA. Temozolomide (TMZ) may have better effects on the treatment of PA infiltrating more immune cells. Anti-PD-L1 therapy may better respond to PA with higher Ki-67, p53 expression and more infiltrating CD68+ macrophages. Multiple treatment modalities, especially combined immunotherapy, or immunotherapy with TMZ combination, could become a novel therapeutic strategy for invasive PA.
RBM24 Mediates Lymph Node Metastasis and Epithelial-Mesenchymal-Transition in Human Hypopharyngeal Squamous Cell Carcinoma Via Regulating Twist1
Authors: Liu Yuhong , Lu Tao , Pan Min , Yu Dan , Li Yanshi , Hu Guohua
Published: December 2021

Background: Hypopharyngeal squamous cell carcinoma (HSCC) has the worst prognosis among head and neck tumours, and Lymph node (LN) metastasis mainly accounts for the poor prognosis. RBM24 (RNA Binding Motif Protein 24) regulates target RNA as an RNA binding protein involved in several cancers. However, its role in HSCC remains completely unknown. Here we attempt to explore the effects of RBM24 on HSCC. Methods: RNA sequencing was conducted to find the differentially expressed genes in tumour tissues from HSCC patients with LN metastasis and without LN metastasis in our previous study. Expression of RBM24 in HSCC tissues was analyzed by qRT-PCR, western blot and immunohistochemistry. Cell proliferation was tested by CCK8 assay as well as Colony formation analysis. Cell migration and invasion capacity were estimated by transwell assay. The wound healing assay was also carried out to evaluate the motility of FaDu cells. QRT-PCR, western blot and immunofluorescence assays were conducted to detect the process of EMT. A popliteal lymph node metastasis model was constructed to explore the effect of RBM24 on HSCC in vivo.Results: RBM24 was remarkably down-regulated in HSCC patients with LN metastasis, and low expression of RBM24 was inextricably linked to the poor prognosis. Knockdown of RBM24 facilitated the proliferation, migration and invasion of RBM24, whereas overexpression of RBM24 showed the opposite effects and suppressed the epithelial-mesenchymal-transition (EMT) process. Overexpression of Twist1 could reverse the inhibitory effects of RBM24 on motility and invasion of FaDu cells. The inhibitory effects of RBM24 on tumour growth and lymphatic metastasis in HSCC were demonstrated by the in vivo experiment as well.Conclusions: These results indicated RBM24 was a suppressor gene and might inhibit EMT and LN metastasis in HSCC via regulating Twist1.