bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening and Functional Analysis of the Peroxiredoxin Specifically Expressed in Bursaphelenchus xylophilus—The Causative Agent of Pine Wilt Disease
Authors: Fu Han-Yu , Ren Jia-Hong , Huang Lin , Li Hao , Ye Jian-Ren
Published: June 2014

The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. Accurately differentiating B. xylophilus from other nematodes species, especially its related species B. mucronatus, is important for pine wood nematode detection. Thus, we attempted to identify a specific protein in the pine wood nematode using proteomics technology. Here, we compared the proteomes of B. xylophilus and B. mucronatus using Two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization -time-of-flight/time-of-flight (MALDI-TOF/TOF-MS) technologies. In total, 15 highly expressed proteins were identified in B. xylophilus compared with B. mucronatus. Subsequently, the specificity of the proteins identified was confirmed by PCR using the genomic DNA of other nematode species. Finally, a gene encoding a specific protein (Bx-Prx) was obtained. This gene was cloned and expressed in E. coli. The in situ hybridisation pattern of Bx-Prx showed that it was expressed strongly in the tail of B. xylophilus. RNAi was used to assess the function of Bx-Prx, the results indicated that the gene was associated with the reproduction and pathogenicity of B. xylophilus. This discovery provides fundamental information for identifying B. xylophilus via a molecular approach. Moreover, the purified recombinant protein has potential as a candidate diagnostic antigen of pine wilt disease, which may lead to a new immunological detection method for the pine wood nematode.
SUMOylation of FOXM1B Alters Its Transcriptional Activity on Regulation of MiR-200 Family and JNK1 in MCF7 Human Breast Cancer Cells
Authors: Wang Chiung-Min , Liu Runhua , Wang Lizhong , Nascimento Leticia , Brennan Victoria , Yang Wei-Hsiung
Published: June 2014

Transcription factor Forkhead Box Protein M1 (FOXM1) is a well-known master regulator in controlling cell-cycle pathways essential for DNA replication and mitosis, as well as cell proliferation. Among the three major isoforms of FOXM1, FOXM1B is highly associated with tumor growth and metastasis. The activities of FOXM1B are modulated by post-translational modifications (PTMs), such as phosphorylation, but whether it is modified by small ubiquitin-related modifier (SUMO) remains unknown. The aim of the current study was to determine whether FOXM1B is post-translationally modified by SUMO proteins and also to identify SUMOylation of FOXM1B on its target gene transcription activity. Here we report that FOXM1B is clearly defined as a SUMO target protein at the cellular levels. Moreover, a SUMOylation protease, SENP2, significantly decreased SUMOylation of FOXM1B. Notably, FOXM1B is selectively SUMOylated at lysine residue 463. While SUMOylation of FOXM1B is required for full repression of its target genes MiR-200b/c and p21, SUMOylation of FOXM1B is essential for full activation of JNK1 gene. Overall, we provide evidence that FOXM1B is post-translationally modified by SUMO and SUMOylation of FOXM1B plays a functional role in regulation of its target gene activities.
Erythropoietin Action in Stress Response, Tissue Maintenance and Metabolism
Authors: Zhang Yuanyuan , Wang Li , Dey Soumyadeep , Alnaeeli Mawadda , Suresh Sukanya , Rogers Heather , Teng Ruifeng , Noguchi Constance
Published: June 2014

Erythropoietin (EPO) regulation of red blood cell production and its induction at reduced oxygen tension provides for the important erythropoietic response to ischemic stress. The cloning and production of recombinant human EPO has led to its clinical use in patients with anemia for two and half decades and has facilitated studies of EPO action. Reports of animal and cell models of ischemic stress in vitro and injury suggest potential EPO benefit beyond red blood cell production including vascular endothelial response to increase nitric oxide production, which facilitates oxygen delivery to brain, heart and other non-hematopoietic tissues. This review discusses these and other reports of EPO action beyond red blood cell production, including EPO response affecting metabolism and obesity in animal models. Observations of EPO activity in cell and animal model systems, including mice with tissue specific deletion of EPO receptor (EpoR), suggest the potential for EPO response in metabolism and disease.
Study on the Synthesis, Biological Activity and Spectroscopy of Naphthalimide-Diamine Conjugates
Authors: Tian Zhi-Yong , Li Jing-Hua , Li Qian , Zang Feng-Lei , Zhao Zhong-Hua , Wang Chao-Jie
Published: June 2014

Eleven novel naphthalimide-diamine conjugates were synthesized and their structures were confirmed by elemental analysis, 1H-NMR, 13C-NMR and MS. Their in vitro antitumor activities were assessed using MTT assays on two cancerous cell lines K562, HCT116, and one normal hepatoma cell line QSG 7701. Compound 7f exhibited potent antitumor activity on HCT116 cells and favorable cell selectivity toward QSG 7701 compared with the positive control, amonafide. Moreover, 7f could block HeG2 cells in the G2/M phase and induce HeG2 cells apoptosis. The interaction of compound 7f with herring sperm DNA was studied by UV/vis absorption and fluorescence spectroscopy under physiological conditions (pH = 7.4). The observed spectral quenching of compound 7f by DNA and the displacement of EB from DNA-EB complex by compound 7f indicated that compound 7f could intercalate into DNA base pairs, which was also corroborated by the effect of KI on compound-DNA interaction. Further caloric fluorescent tests revealed that the quenching mechanism was a static type. Meanwhile, the binding constants, thermodynamic parameters and the effect of NaCl on compound-DNA interaction showed that the type of interaction force was mainly hydrogen bonds and the binding process was driven by hydrogen and van der Waals bonding.
Salidroside Protects Caenorhabditis elegans Neurons from Polyglutamine-Mediated Toxicity by Reducing Oxidative Stress
Authors: Xiao Lingyun , Li Haifeng , Zhang Ju , Yang Fan , Huang Aizhen , Deng Jingjing , Liang Ming , Ma Fangli , Hu Minghua , Huang Zebo
Published: June 2014

Polyglutamine (polyQ) aggregation plays a pivotal role in the pathological process of Huntington’s disease and other polyQ disorders. Therefore, strategies aiming at restoring dysfunction and reducing stresses mediated by polyQ toxicity are of therapeutic interest for proteotoxicity diseases. Salidroside, a glycoside from Rhodiola rosea, has been shown to have a variety of bioactivities, including antioxidant activity. Using transgenic Caenorhabditis elegans models, we show here that salidroside is able to reduce neuronal death and behavioral dysfunction mediated by polyQ expressed in ASH neurons, but the neuroprotective effect is not associated with prevention of polyQ aggregation per se. Further experiments reveal that the neuroprotective effect of salidroside in C. elegans models involves its antioxidant capabilities, including decrease of ROS levels and paraquat-induced mortality, increase of antioxidant enzyme activities and reduction of lipid peroxidation. These results demonstrate that salidroside exerts its neuroprotective function against polyQ toxicity via oxidative stress pathways.
Antimicrobial Peptides in Reptiles
Authors: Van Hoek Monique
Published: June 2014

Reptiles are among the oldest known amniotes and are highly diverse in their morphology and ecological niches. These animals have an evolutionarily ancient innate-immune system that is of great interest to scientists trying to identify new and useful antimicrobial peptides. Significant work in the last decade in the fields of biochemistry, proteomics and genomics has begun to reveal the complexity of reptilian antimicrobial peptides. Here, the current knowledge about antimicrobial peptides in reptiles is reviewed, with specific examples in each of the four orders: Testudines (turtles and tortosises), Sphenodontia (tuataras), Squamata (snakes and lizards), and Crocodilia (crocodilans). Examples are presented of the major classes of antimicrobial peptides expressed by reptiles including defensins, cathelicidins, liver-expressed peptides (hepcidin and LEAP-2), lysozyme, crotamine, and others. Some of these peptides have been identified and tested for their antibacterial or antiviral activity; others are only predicted as possible genes from genomic sequencing. Bioinformatic analysis of the reptile genomes is presented, revealing many predicted candidate antimicrobial peptides genes across this diverse class. The study of how these ancient creatures use antimicrobial peptides within their innate immune systems may reveal new understandings of our mammalian innate immune system and may also provide new and powerful antimicrobial peptides as scaffolds for potential therapeutic development.
The Effect of CFRP Length on the Failure Mode of Strengthened Concrete Beams
Authors: Ding Jun , Wang Fang , Huang Xia , Chen Song
Published: June 2014

This paper reports the effects of carbon fiber-reinforced polymer (CFRP) length on the failure process, pattern and crack propagation for a strengthened concrete beam with an initial notch. The experiments measuring load-bearing capacity for concrete beams with various CFRP lengths have been performed, wherein the crack opening displacements (COD) at the initial notch are also measured. The application of CFRP can significantly improve the load-bearing capacity, and the failure modes seem different with various CFRP lengths. The stress profiles in the concrete material around the crack tip, at the end of CFRP and at the interface between the concrete and CFRP are then calculated using the finite element method. The experiment measurements are validated by theoretical derivation and also support the finite element analysis. The results show that CFRP can significantly increase the ultimate load of the beam, while such an increase stops as the length reaches 0.15 m. It is also concluded that the CFRP length can influence the stress distribution at three critical stress regions for strengthened concrete beams. However, the optimum CFRP lengths vary with different critical stress regions. For the region around the crack tip, it is 0.15 m; for the region at the interface it is 0.25 m, and for the region at the end of CFRP, it is 0.30 m. In conclusion, the optimum CFRP length in this work is 0.30 m, at which CFRP strengthening is fully functioning, which thus provides a good reference for the retrofitting of buildings.
Joint Target Tracking, Recognition and Segmentation for Infrared Imagery Using a Shape Manifold-Based Level Set
Authors: Gong Jiulu , Fan Guoliang , Yu Liangjiang , Havlicek Joseph , Chen Derong , Fan Ningjun
Published: June 2014

We propose a new integrated target tracking, recognition and segmentation algorithm, called ATR-Seg, for infrared imagery. ATR-Seg is formulated in a probabilistic shape-aware level set framework that incorporates a joint view-identity manifold (JVIM) for target shape modeling. As a shape generative model, JVIM features a unified manifold structure in the latent space that is embedded with one view-independent identity manifold and infinite identity-dependent view manifolds. In the ATR-Seg algorithm, the ATR problem formulated as a sequential level-set optimization process over the latent space of JVIM, so that tracking and recognition can be jointly optimized via implicit shape matching where target segmentation is achieved as a by-product without any pre-processing or feature extraction. Experimental results on the recently released SENSIAC ATR database demonstrate the advantages and effectiveness of ATR-Seg over two recent ATR algorithms that involve explicit shape matching.
Exploring Sustainable Urban Food Provisioning: The Case of Eggs in Dar es Salaam
Authors: Wegerif Marc
Published: June 2014

Global food supply is dominated by transnational corporations, which have great power and are widely critiqued for the negative environmental and social impacts of their operations. Many argue that this industrial food system is unsustainable, yet its expansion seems inevitable and alternatives are seen as incapable of feeding the world’s growing and increasingly urban population. Since much of the world’s future population growth is going to happen in the cities of the developing world, they have become the frontline for the expansion of the industrial food system, raising the serious challenge of ensuring food security for residents. This paper, based on a qualitative study of patterns of egg provisioning in Dar es Salaam, explores whether existing patterns of food supply in this rapidly growing city, of over four million people, provide workable alternatives. Eggs are an important source of nutrition and patterns of egg supply offer a lens through which to explore the sustainability of different modes of provisioning. A range of non-corporate provisioning patterns, based on small-scale enterprises, are found to have social, economic and environmental advantages, challenging assumptions that corporate food chains are necessary, or desirable, to feed cities sustainably.
Further Insights into Brevetoxin Metabolism by de Novo Radiolabeling
Authors: Calabro Kevin , Guigonis Jean-Marie , Teyssié Jean-Louis , Oberhänsli François , Goudour Jean-Pierre , Warnau Michel , Bottein Marie-Yasmine , Thomas Olivier
Published: June 2014

The toxic dinoflagellate Karenia brevis, responsible for early harmful algal blooms in the Gulf of Mexico, produces many secondary metabolites, including potent neurotoxins called brevetoxins (PbTx). These compounds have been identified as toxic agents for humans, and they are also responsible for the deaths of several marine organisms. The overall biosynthesis of these highly complex metabolites has not been fully ascertained, even if there is little doubt on a polyketide origin. In addition to gaining some insights into the metabolic events involved in the biosynthesis of these compounds, feeding studies with labeled precursors helps to discriminate between the de novo biosynthesis of toxins and conversion of stored intermediates into final toxic products in the response to environmental stresses. In this context, the use of radiolabeled precursors is well suited as it allows working with the highest sensitive techniques and consequently with a minor amount of cultured dinoflagellates. We were then able to incorporate [U-14C]-acetate, the renowned precursor of the polyketide pathway, in several PbTx produced by K. brevis. The specific activities of PbTx-1, -2, -3, and -7, identified by High-Resolution Electrospray Ionization Mass Spectrometer (HRESIMS), were assessed by HPLC-UV and highly sensitive Radio-TLC counting. We demonstrated that working at close to natural concentrations of acetate is a requirement for biosynthetic studies, highlighting the importance of highly sensitive radiolabeling feeding experiments. Quantification of the specific activity of the four, targeted toxins led us to propose that PbTx-1 and PbTx-2 aldehydes originate from oxidation of the primary alcohols of PbTx-7 and PbTx-3, respectively. This approach will open the way for a better comprehension of the metabolic pathways leading to PbTx but also to a better understanding of their regulation by environmental factors.
Designing Vaccines to Neutralize Effective Toxin Delivery by Enterotoxigenic Escherichia coli
Authors: Fleckenstein James , Sheikh Alaullah
Published: June 2014

Enterotoxigenic Escherichia coli (ETEC) are a leading cause of diarrheal illness in developing countries. Despite the discovery of these pathogens as a cause of cholera-like diarrhea over 40 years ago, and decades of vaccine development effort, there remains no broadly protective ETEC vaccine. The discovery of new virulence proteins and an improved appreciation of the complexity of the molecular events required for effective toxin delivery may provide additional avenues to pursue in development of an effective vaccine to prevent severe diarrhea caused by these important pathogens.
Non-Human Primate Models of Orthopoxvirus Infections
Authors: Schmitt Anne , Mätz-Rensing Kerstin , Kaup Franz-Josef
Published: June 2014

Smallpox, one of the most destructive diseases, has been successfully eradicated through a worldwide vaccination campaign. Since immunization programs have been stopped, the number of people with vaccinia virus induced immunity is declining. This leads to an increase in orthopoxvirus (OPXV) infections in humans, as well as in animals. Additionally, potential abuse of Variola virus (VARV), the causative agent of smallpox, or monkeypox virus, as agents of bioterrorism, has renewed interest in development of antiviral therapeutics and of safer vaccines. Due to its high risk potential, research with VARV is restricted to two laboratories worldwide. Therefore, numerous animal models of other OPXV infections have been developed in the last decades. Non-human primates are especially suitable due to their close relationship to humans. This article provides a review about on non-human primate models of orthopoxvirus infections.
Membrane phospholipid redistribution in cancer micro‐particles and implications in the recruitment of cationic protein factors
Authors: Hou Songwang , Grillo Doris , Williams Carol L. , Wasserstrom J. Andrew , Szleifer Igal , Zhao Ming
Published: June 2014

Cancer cell‐derived micro‐particles (MPs) play important regulatory roles on cellular and system levels. These activities are attributed in part to protein factors carried by MPs. However, recruitment strategies for sequestering certain protein factors in MPs are poorly understood. In the current study, using exogenous and endogenously expressed phospholipid‐binding probes, we investigated the distribution of membrane phospholipids in MPs as a potential mechanism for electrostatically enriching cationic protein factors in MPs. We detected a significant level of externalised phosphatidylethanolamine (PE) at the outer surface of MPs. This was accompanied, in the inner leaflet of the MP membrane, by a greater density of negatively charged phospholipids, particularly phosphatidylserine (PS). The local enrichment of PS in the inner surface of MPs was correlated with an elevated presence of small GTPases in a polybasic region (PBR)‐dependent fashion. By employing a series of RhoA derivatives, including constitutively active and RhoA derivatives lacking a PBR, we could demonstrate that the congregation of RhoA in MPs was dependent on the presence of the PBR. A chimer with the fusion of PBR sequence alone to GFP significantly enhanced GFP localisation in MPs, indicative of a positive contribution of electrostatic interactions in RhoA recruitment to MPs. Using in silico thermodynamic simulations, we characterised the electrostatic interactions between PBR and anionic lipid membrane surface. In summary, the redistribution of membrane phospholipids in MPs has an impact on the local ionic density, and is likely a contributing factor in the electrostatic recruitment of membrane‐associated proteins to MPs in a PBR‐dependent fashion.
Measuring air layer volumes retained by submerged floating-ferns Salvinia and biomimetic superhydrophobic surfaces
Authors: Mayser Matthias J , Bohn Holger F , Reker Meike , Barthlott Wilhelm
Published: June 2014

Some plants and animals feature superhydrophobic surfaces capable of retaining a layer of air when submerged under water. Long-term air retaining surfaces (Salvinia-effect) are of high interest for biomimetic applications like drag reduction in ship coatings of up to 30%. Here we present a novel method for measuring air volumes and air loss under water. We recorded the buoyancy force of the air layer on leaf surfaces of four different Salvinia species and on one biomimetic surface using a highly sensitive custom made strain gauge force transducer setup. The volume of air held by a surface was quantified by comparing the buoyancy force of the specimen with and then without an air layer. Air volumes retained by the Salvinia-surfaces ranged between 0.15 and 1 L/m2 depending on differences in surface architecture. We verified the precision of the method by comparing the measured air volumes with theoretical volume calculations and could find a good agreement between both values. In this context we present techniques to calculate air volumes on surfaces with complex microstructures. The introduced method also allows to measure decrease or increase of air layers with high accuracy in real-time to understand dynamic processes.
Severity of Liver Disease Affects HCV Kinetics in Patients Treated with Intravenous Silibinin Monotherapy
Authors: Canini Laetitia , DebRoy Swati , Mariño Zoe , Conway Jessica M , Crespo Gonzalo , Navasa Miquel , D'Amato Massimo , Ferenci Peter , Cotler Scott J , Forns Xavier , Perelson Alan S , Dahari Harel
Published: June 2014

Background

HCV kinetic analysis and modelling during antiviral therapy have not been performed in decompensated cirrhotic patients awaiting liver transplantation. Here, viral and host parameters were compared in three groups of patients treated with daily intravenous silibinin (SIL) monotherapy for 7 days according to the severity of their liver disease.

Methods

Data were obtained from 25 patients, 12 non-cirrhotic, 8 with compensated cirrhosis and 5 with decompensated cirrhosis. The standard-biphasic model with time-varying SIL effectiveness (from 0 to final effectiveness [εmax]) was fitted to viral kinetic data.

Results

Baseline viral load and age were significantly associated with the severity of liver disease ( P<0.0001). A biphasic viral decline was observed in most patients with a higher first phase decline in patients with less severe liver disease. The εmax was significantly ( P≤0.032) associated with increasing severity of liver disease (non-cirrhotic εmax [se]=0.86 [0.05], compensated cirrhotic εmax=0.69 [0.06] and decompensated cirrhotic εmax=0.59 [0.1]). The second phase decline slope was not significantly different among groups (mean 1.88 ±0.15 log10 IU/ml/week, P=0.75) as was the rate of change of SIL effectiveness (k=2.12/day [se=0.18/day]). HCV-infected cell loss rate (δ [se]=0.62/ day [0.05/day]) was high and similar among groups.

Conclusions

The high loss rate of HCV-infected cells suggests that sufficient dose and duration of SIL might achieve viral suppression in advanced liver disease.
Dynamiques identitaires d'acteurs de l'éducationen période de transition
Authors: Perez-Roux Thérèse
Published: June 2014

Résumé Cet article analyse les transitions professionnelles vécues par d’anciens enseignants spécialisés évoluant vers des fonctions d’Enseignants Référents pour le suivi d’élèves en situation de handicap. Il montre que ces nouveaux acteurs de l’éducation peuvent mobiliser un certain nombre de compétences et ressources développées en tant qu’enseignant spécialisé pour investir cette fonction de coordination et de médiation. Il rend également compte des ruptures et déstabilisations qui impliquent la construction d’un nouveau rapport au métier, rendu possible par l’élaboration collective entre pairs d’une culture professionnelle et par la reconnaissance institutionnelle de l’autonomie de ces professionnels.
La création d'entreprise dans le secteur du tourisme sportif
Authors: Dubois Fanny , Terral Philippe
Published: June 2014

Résumé partir d’entretiens biographiques et directifsmenés auprès de cinquantetrois dirigeants d’entreprises de tourisme sportif de nature, cet article tente de comprendre le processus de transition propre à la création de ces entreprises, le caractère passionné des professionnels avec ces activités ne s’associant pas aisément avec leur marchandisation. Il met en évidence trois figures de transition. Les transformations identitaires vécues par les acteurs et les aspects plus concrets caractérisant les processus transitionnels révèlent la nécessité de dissocier la dimension objective de la dimension subjective dans l’analyse des transitions professionnelles.
Les transitions professionnelles des personnes touchées par une maladie chronique
Authors: Waser Anne-Marie , Lénel Pierre
Published: June 2014

Résumé À partir de l’analyse d’un matériau issu de deux recherches-actions auprès de personnes touchées par des maladies chroniques qui avaient exprimé le souhait de retrouver une activité, cet article propose une thématisation des transitions professionnelles sous l’aspect d’une rupture avec les normes sociales du travail. Les participants, à travers différentes stratégies, montrent qu’ils cherchent à agir non seulement sur les conditions de leur santé, sur les conditions de collaboration avec les autres, mais aussi à faire évoluer les normes du travail. Ainsi à l’occasion d’un dispositif d’enquête particulier, la rupture biographique se « transmeut » en une dynamique de transformation sociale.
First report of mycophenolic acid production byEurotium repens isolated from agricultural and indoor environments
Authors: Séguin V. , Gente S. , Heutte N. , Vérité P. , Kientz-Bouchart V. , Sage L. , Goux D. , Garon D.
Published: June 2014

Mycophenolic acid is considered as an immunosuppressive pharmaceutical drug, but also as a mycotoxin due to its undesirable presence in various feedstuffs. AlthoughPenicillium brevicompactum has been historically identified as the major producer of mycophenolic acid, this study reports for the first time the production of mycophenolic acid by isolates ofEurotium repens collected from agricultural and indoor environments. Fungal identification was achieved by scanning electron microscopy and molecular techniques, and mycophenolic acid production was confirmed by two analytical methods (gas and liquid chromatography coupled to mass spectrometry). These findings suggest that mycophenolic acid production and immunosuppressive properties of other closely relatedEurotium should be evaluated in future studies.
Création de dictionnaires bilingues spécialistes : l’épreuve de vérité du retournement informatique
Authors: Schuwer Martine
Published: June 2014

Cet exposé constitue la seconde partie de la méthodologie de création de dictionnaires bilingues spécialisés consacrée à la réalisation de la version anglais-français, et élaborée à l’aide d’un logiciel spécifique de gestion de données. Lors de la mise en œuvre de la partie français-anglais, réalisée informatiquement, sont apparus de multiples problèmes inattendus : certains, d’ordre technique, peuvent bénéficier d’améliorations en amont, mais pour les autres, il n’existe pas de solution d’ensemble car ils sont en réalité le révélateur sévère de la non-correspondance des champs sémantiques d’une langue à l’autre. Le retournement informatique constitue donc une « épreuve de vérité » en ce sens qu’il met en lumière les « vices cachés » (lacunes/approximations) de la version de départ ; il souligne par ailleurs les efforts délibérés ou inconscients que le lexicographe déploie lors de la mise en équivalence afin de procurer à l’utilisateur un outil aussi fiable et complet que possible.
A Research on the Weight of Interactive Multiple Model in Maneuvering Target Tracking
Authors: Liu Ming Yong , Li Yang , Zhang Xiao Jian
Published: June 2014

The establishment of the target model is the key of maneuvering target tracking. The previous research on interactive multiple model, which is applied on tracking extensively, focused on the design of the model set and fusion with other algorithms, while there is less study on change mechanisms of the model weight. In light of this, the impetus behind this paper is to do some analysis which based on the model weight of different trajectories, reveal the change rule. Finally, the validity of the proposed approach is demonstrated by simulation.
An Antenna Tracking Servo System Based on Particle Filtering
Authors: Guo Rui , Yin Xing Hui , Xu Li Zhong
Published: June 2014

To track the moving target with the nonlinear and non-Gaussian state in real time, we in this paper apply particle filtering theory in the antenna tracking system. After the prediction of the target state, the antenna attitude is regulated in real time by the servo control loop so that the antenna can aim at the target precisely. The particle filtering can efficiently improve the tracking performance of the antenna tracking servo system affected by the delay time in comparison with Extended Kalman Filtering. The system performance is verified by a numerical simulation of maneuvering target tracking.
Fuzzy PID Control for Fast-Steering Mirror System
Authors: Bai Xue Jin , Qiao Yong Ming
Published: June 2014

Fast-steering mirror system demands higher accuracy and fast responding speed to track targets, but the conventional PID controllers cannot meet the demands. Instead, the fuzzy PID control can greatly improve the capturing and tracking capacities to high-speed dynamic targets. So we apply the fuzzy-PID controller in the positioning loop of the stabilized system, not only improving the transient process of the control system and decreasing the overshoot, but also enhancing the accuracy of tracking stabilization and response. At the end, simulations were performed to test the effectiveness of this method through the MATLAB platform.
Research on the Real-Time Model of Aero-Engine Actuator
Authors: Li Hua Cong , Zhang Hong An , Han Xiao Bao , Fu Jiang Feng
Published: June 2014

Since the solving process of hydraulic dynamic simulation is complex and computational ineffectiveness,the aero-engine actuators real-time modeling is presented in this paper. Combined with the precise model, the convergence of the model and flow coefficient is analyzed. The real-time model operates a number of solving processes in one 20ms simulation cycle and the convergence of fix-step algorithm is guaranteed by adjusting the relevant parameters. The simulation shows that the real-time model can improve the computational efficiency with satisfactory real-time performance and precision.
A LPV Network Control System Fault Detection Based on H∞ Control Theory
Authors: Gao Zhen Bin
Published: June 2014

This paper considers the problem of a polytopic approach for Linear parameter Variation network control system with the sensor failure case. Based on Bounded Real Lemma of control theory, the sufficient condition of robust stability of a LPV augmented network control system with tracking error and the sensor failure is addressed; Using Linear Matrix Inequality convex optimal technique, the feasible solution of state feedback controller is obtained. The simulation of a inverted pendulum model shows that the presented method is feasible and effective.
The Environment Monitoring System of Refuge Cabin and its Simulation Based on Fuzzy-PID Algorithm
Authors: Yan Huai Chao , Li Lin Jie , Huang Jun Ge
Published: June 2014

In order to improve people's chances of survival, Multifunctional fire refuge compartment, a kind of emergency equipment, was invented when a high-rise building breaking out of fire. One key component of it is the environmental monitoring system of refuge cabin. Meanwhile, to ensure that the refuge chamber system can respond quickly and be stable operation, it takes an kind of intelligent control technology which combined with traditional PID control(Proportion、Integration、Differentiation)and fuzzy control technology, namely fuzzy PID control to regulate. In this paper, combined with the fuzzy control technology, we discuss the fuzzy self-tuning PID control method of refuge cabin environment monitoring system. Try to make digital processing of control system by MCU(Micro Control Unit), we use the Simulink and Fuzzy toolbox in MATLAB to simulate the refuge compartment cooling system which using the fuzzy PID control method and compare with the conventional PID control. The result shows that Fuzzy-PID control method reduces the overshoot of the system, shortens the settling time, decreases steady-state deviation, improves the static and dynamic characteristics of the system effectively. Finally, it can enhance the reliability and adaptability of the system.
The Overall Training Evaluation of Command and Control System
Authors: Mao Jian Zhou , Zhang Hao , Wang Yan Hui , Li Yu Bao
Published: June 2014

This paper proposed a set of evaluation index system to assess the holistic training effectiveness of the ship-borne command and control system, the criteria and computing method of each index are provided, the weights value of each index are analyzed and calculated, and provided a set of method to integrate the evaluation index for the evaluation system. The method could be used to promote the training and combat effectiveness.
Design of Automated Catwalks Machine Control System for Offshore Drilling Platform
Authors: Qi Shu Fen , Cheng Shi Ping , Wang Wen Long , Zhang Kun
Published: June 2014

In recent years, the use of drill string automated processing systems on offshore drilling platform has become more and more widespread, and it not only can improve the efficiency of workers, but also can reduce the labor intensity. In order to make the automatic system more intelligent and more efficient in the whole process, in this paper, we designed a set of adjustable speed drill delivery system, which is based on PID control algorithm. It can not only improve the efficiency of the automated catwalks machine conveying pipe, but also ensure the quick and efficient production.
Design of Remote Destruction SSD
Authors: Yu Yi , Xu Xin , Zheng Guo
Published: June 2014

An implementation of a remote destruction solid state drive (SSD) was introduced in the design. The advanced technology of rapid data destruction, the technology of breakdown the flash with high-voltage pulse and the technology of GSM is combined together. As a result, user can operate through a simple command (text message or a call) to destroy all data in SSD, as long as the user confirms SSD is out of control. The method of erasing data and destroying the flash I/O are both executed, so the data can’t be recovered after destruction. At last, some tests are displayed to verify the performance of the remote destruction SSD.
Research and Implementation of SSD Lifespan Protection Mechanism
Authors: Liu Chao , Li Nan , Xu Xin
Published: June 2014

With the continuous development of SSD, It becomes the commodity requirements of memory storage users especially in consumer application. NAND Flash lifespan limitation is the most concerns of every user in relation to the drive’s reliability. This paper explains three factors which embark from the basic principle effect of SSD lifespan and induced the reliability strategies/the method of SSD lifespan prediction during the SSD design and usage period. We have designed a SSD`s lifespan management software which predict and manage the SSD lifespan. It will enhance the safety of SSD usage.SSD lifespan mechanism analyzes how actively you use your solid-state drive and uses a special algorithm to calculate its estimated lifetime. Of course, the date of the lifetime expiration is corrected depending on how intensively you keep using your drive.
Design and Simulation of High-Frequency Track Circuit for Rail Transportation Experimental System
Authors: Hu Chun Hong , Tian Yuan , Zhai Guo Rui , He Jun
Published: June 2014

Rail transportation experimental system is the urgent need of modern railway signal professional colleges and universities teaching equipment. This equipment can deepen students' understanding of theoretical knowledge and improve their ability of practical operation. In this article the design schemes of track circuit for high-frequency test system are simulated. Through the result of simulation the design meet the functional requirement.
Approaches on the Calibration of Laser Diode (LD) Tester
Authors: Zhan Zhi Qiang , Xia Jun Wen , Yu Lei
Published: June 2014

Laser Diode (LD) tester is widely used in the research, manufacturing and testing of laser diode, but no verification regulation or calibration specification for laser diode tester has been released until now. With the fully using of the standard instruments in our optical communication laboratory, and referring to the operational manual, specification and relevant calibration regulations, we provide approaches to the calibration of the laser diode tester. The evaluation processes and results of measurement uncertainty for optical power measurement, output DC current, and DC voltage measurement are delivered at the end of the paper.
Fiber-Optics Data Transmission for a Gyro Stabilized Platform
Authors: Han Di , Li Hai Xia
Published: June 2014

An optical fiber based data transmission link for a four-axis gyro stabilized inertial reference system is presented. The operation and configuration of the gyro stabilized platform is introduced to explain the requirement of data transmission. Four passive fiber optic rotary joints (FORJs) instead of traditional electrical sliprings are used to transmit optical signals between these relatively rotating gimbals. The hardware and software design of this optical fiber communication system operated at an updating rate of 1kHz are described to achieve high-speed, real-time, reliable data transmission. The experimental results show that the fiber optic rotary joint-based data transmission link is real-time and reliable. The use of FORJs as fiber optic sliprings will also result in an extremely low friction torque by comparing to electrical slipring assemblies. This allows the system designer to optimize the servo control performance for such high-precision gyro stabilized inertial reference systems.
Research on Automatic Technology of Debugging and Assembling Optical Fiber Collimator
Authors: Wang Xiao Hua , Zheng Qiu Xin , Zhao Qian , Li Li , Guo Jian Qin
Published: June 2014

In order to improve the efficiency of debugging and assembling optical fiber collimator and to facilitate mass production, Automatic assembly technology of optical fiber collimator was studied. A new method was proposed to assemble optical fiber collimator by detecting minimal receiving beam spot. First, the relation was analyzed between the detected spot radius and the distance from the emitting end to reception end according to Gaussian beam transmission theory. Then, an automation system was designed to detect the minimal receiving beam spot when the distance was suitable. The optical fiber collimator was successfully assembled after being fixed. Experimental results show that the coupling loss between two collimators is less than 0.53dB when the wavelength of the transmitting light is 1550nm and the distance between two collimators is less than 1 mm.
Complexity Evaluation Method of Electromagnetic Environment Oriented to Communication Equipment Based on Statistic Theory
Authors: Zhang Shao Jun , Su Dong Lin , Wang Jian , Wang Rui
Published: June 2014

To improve the objectivity and accuracy of evaluation for electromagnetic environment complexity and its influence on communication equipment, a subjective complexity evaluation method of electromagnetic environment oriented to the communication equipment is presented based on sensitivity and RF protection ratio of communication equipment. Based on statistical concept, the energy correlation factor is modified, and modulation and polarization correlation concept are imported into this method, by which the factor of energy correlation is modified and the subjective evaluation is made by analyzing the time domain correlation, frequency domain correlation, energy domain correlation, modulation correlation and polarization correlation of the electromagnetic environment. These simulation results indicate that communication equipment with various working parameters are influenced differently under the same electromagnetic environment. Namely, the complexities of electromagnetic environment for communication equipment with various working parameters are different.
Construction of Network Teaching Platform
Authors: Kou Yuan Yuan
Published: June 2014

This article discusses the design model, general construction and implement scheme of network teaching platform based on Web techniques, probes the application of education informationization in the teaching process. Combined modern teaching theory and multi-medium techniques, the article demonstrates and implements the main function and demand of network teaching platform from m teaching and learning point of view.
Planning of Network Communication System of Yalong River Basin Hydropower Development Co., LTD
Authors: Cai Hua Long , Gu Bing , Wu Zhi Yu
Published: June 2014

The planning of network communications of Yalong river basin hydropower development company aims to adapt to the company's strategy and making decision, meet the requirements of project construction, electric power production and administrative management. Based on the principle of advanced nature, economy, reliability, security, scalability, and standardization, the concept of basin area and framework of network communication are put forward on the regional development and project category of Yalong River Company . The content of communication planning is to solve the network communication problems of the company headquarters, between the company headquarters and secondary units focus on office, between corporate headquarters and production, between basin area and each power plant under construction projects.
Multi-Agent Fault Diagnosis Methods Based on Information Fusion
Authors: Lv Peng Liang , Chen Guo Shun
Published: June 2014

In order to meet the current needs of complex technical equipment maintenance and support, the paper member with the structural characteristics of Multi-Agent System, which was introduced to the fault diagnosis method based on information fusion, the main research was distributed intelligent monitoring and diagnosis system framework based on information fusion, and analysis of information fusion method for the diagnosis of strategies for the a typical system feature, including the contents of the implementation of the method and research status, and points out its future research directions.
On Energy Consumption of Network Coding Based Reliability Transmission Mechanism
Authors: Hu Peng , Song Xiao Quan
Published: June 2014

Network coding based multipath routing (NCMR) transmission mechanism is interpreted. Introduced the energy model into research, the transmission reliability and energy consumption of NCMR is analyzed with quantitative analysis on a braided multipath network model. A source controlling strategy is proposed for optimizing the energy consumption with transmission reliability requirement.
Design and Implementation of Network Events Monitoring System
Authors: Wang Chun Ping
Published: June 2014

The model of network monitoring system proposed in this paper, the use of user modeling techniques and event detection techniques. Preclude the use of dynamic modeling and dynamic model of the method of combining inferred more detailed user interest model to optimize the results, event detection method for the introduction of natural language processing, the system automatically send the text to identify hot topics and events advertising. In the system design, considering the intersection of the two, to obtain a better user experience.
Implementation of a Customiazble GUI Software Platform for IC Equipment
Authors: Wang Chen Xi , Liu Ming Zhe , Xu Ai Dong
Published: June 2014

With the development of semiconductor industry, different types of wafer processing are increasing. According to the different wafer processing models, the need of data display and process is different. In this paper, a customizable software platform is described for the manufacturing equipment of semiconductor integrated circuit (IC equipment). The C# control technology has been used to build the IC equipment customizable control system interface. The development method of customizable control technology based on C# can realize the control of the reuse and codes sharing, in order to improve programming efficiency, avoid the development of two times, cost saving and be easy to debug.
Research on Automatic Test Case Generation Framework for Java
Authors: Xu Ming Gang , Mu Yong Min , Zhang Zhi Hua , Liu Ang
Published: June 2014

Automatic test case generation has been a hotspot and a difficult problem in the software testing, Accurately and efficiently generate test cases can improve the efficiency of software testing. Java programs have many characteristics such as encapsulation, inheritance, polymorphism and so on, it is convenient for software design and development, but to bring automated testing some difficulties. This article on the Java program of automatic test case generation method is studied and presents a framework for automatic generation of test cases. With this framework, test case suite will be generated quickly and accurately. Experimental results show that automatic Java test case generation framework can quickly and accurately generate test cases , reduce labor costs and improve efficiency.
Generation of Static Function Calling Paths in C++ Based on Finite-State Machine
Authors: Liu Dan Feng , Mu Yong Min , He Yu Jie , Liu Meng Ting
Published: June 2014

Path coverage testing based on the function calling relationship is an important criterion for ensuring testing adequacy. The key to path testing is the determination of path number. This paper proposes an algorithm to generate static function calling paths with control logic based on the finite-state machine (FSM). The algorithm analyzes the C++ static source code and extracts the program information, generating static function calling paths and function calling graphs. The experiment proves that the algorithm can accurately generate static function calling paths with control logic and obtain the path number of the testing program. The accurate extraction of the static function calling paths lays a solid foundation for path coverage testing based on the function calling relationship.
A Visual Simulation Method for 2-D Complex Situation of Electromagnetic Environment
Authors: Hu Meng Zhong , Yi Tong Sheng , Pan Jia Liang
Published: June 2014

As the complex situation of electromagnetic environment is difficult to be analyzed and simulated visibly, interface program is developed based on the calculation module of Wireless Insite2.5 software. Equipments such as communication, radar, electronics resists and other radio devices are studied to build accurate theoretic models. Moreover, a method for creating software receivers quickly in MATLAB is proposed and other equipment models are created automatically just based on the parameters of equipments in the same way. Then, situations of time, frequency and energy-domain are calculated successfully with using Wireless Insite2.5. Complex 2-D situation of electromagnetic environment is displayed in the 2-D map by using MATLAB map display technology. At last, Display and query visualization for complex situation of electromagnetic environment is realized by designing a visual data query tool of mouse.
Finding k-Dominant Skyline for Combined Dataset
Authors: Dong Lei Gang , Cui Xiao Wei
Published: June 2014

The k-dominant skyline query which can return small result set in high dimensional space is very convenient for user to make decision. But all the existent algorithms are for single dataset, not suitable for two or more datasets being combined. To solve this problem, this paper proposed a new idea to get the new k-dominant skyline by computing the partial points of each dataset based on their existent k-dominant skylines. Furthermore, detailed theoretical analyses and extensive experiments demonstrate that the algorithms can effectively reduce redundant work, and keep the result correctly.