bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone
Authors: Gonçalves Maria Carolina Pereira , Amaral Jéssica Cristina , Fernandez-Lafuente Roberto , Sousa Junior Ruy de , Tardioli Paulo Waldir
Published: June 2021

In this paper, we have performed the Lipozyme 435-catalyzed synthesis of xylose oleate in methyl ethyl ketone (MEK) from xylose and oleic acid. The effects of substrates’ molar ratios, reaction temperature, reaction time on esterification rates, and Lipozyme 435 reuse were studied. Results showed that an excess of oleic acid (xylose: oleic acid molar ratio of 1:5) significantly favored the reaction, yielding 98% of xylose conversion and 31% oleic acid conversion after 24 h-reaction (mainly to xylose mono- and dioleate, as confirmed by mass spectrometry). The highest Lipozyme 435 activities occurred between 55 and 70 °C. The predicted Ping Pong Bi Bi kinetic model fitted very well to the experimental data and there was no evidence of inhibitions in the range assessed. The reaction product was purified and presented an emulsion capacity close to that of a commercial sugar ester detergent. Finally, the repeated use of Lipozyme 435 showed a reduction in the reaction yields (by 48 and 19% in the xylose and oleic acid conversions, respectively), after ten 12 h-cycles.
BDNF as a Putative Target for Standardized Extract of Ginkgo biloba-Induced Persistence of Object Recognition Memory
Authors: Muratori Beatriz G. , Zamberlam Cláudia R. , Mendes Thaís B. , Nozima Bruno H. N. , Cerutti Janete M. , Cerutti Suzete M.
Published: June 2021

Despite considerable progress on the study of the effect of standardized extract of Gingko biloba (EGb) on memory processes, our understanding of its role in the persistence of long-term memory (LTM) and the molecular mechanism underlying its effect, particularly episodic-like memory, is limited. We here investigated the effects of EGb on the long-term retention of recognition memory and its persistence and BDNF expression levels in the dorsal hippocampal formation (DHF). Adult male Wistar rats (n = 10/group) were handled for 10 min/5 day. On day 6, the animals were treated with vehicle or 0.4 mg/kg diazepam (control groups) or with EGb (250, 500 or 100 mg/kg) 30 min before the training session (TR1), in which the animals were exposed to two sample objects. On day 7, all rats underwent a second training session (TR2) as described in the TR1 but without drug treatment. Object recognition memory (ORM) was evaluated on day 8 (retention test, T1) and day 9 (persistence test, T2). At the end of T1or T2, animals were decapitated, and DHF samples were frozen at −80 °C for analyses of the differential expression of BDNF by Western blotting. EGb-treated groups spent more time exploring the novel object in T2 and showed the highest recognition index (RI) values during the T1 and T2, which was associated with upregulation of BDNF expression in the DHF in a dose-and session-dependent manner. Our data reveal, for the first time, that EGb treatment before acquisition of ORM promotes persistence of LTM by BDNF differential expression.
PEP-1-GLRX1 Reduces Dopaminergic Neuronal Cell Loss by Modulating MAPK and Apoptosis Signaling in Parkinson’s Disease
Authors: Choi Yeon Joo , Kim Dae Won , Shin Min Jea , Yeo Hyeon Ji , Yeo Eun Ji , Lee Lee Re , Song Yejin , Kim Duk-Soo , Han Kyu Hyung , Park Jinseu , Lee Keun Wook , Park Jong Kook , Eum Won Sik , Choi Soo Young
Published: June 2021

Parkinson’s disease (PD) is characterized mainly by the loss of dopaminergic neurons in the substantia nigra (SN) mediated via oxidative stress. Although glutaredoxin-1 (GLRX1) is known as one of the antioxidants involved in cell survival, the effects of GLRX1 on PD are still unclear. In this study, we investigated whether cell-permeable PEP-1-GLRX1 inhibits dopaminergic neuronal cell death induced by 1-methyl-4-phenylpyridinium (MPP+) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We showed that PEP-1-GLRX1 protects cell death and DNA damage in MPP+-exposed SH-SY5Y cells via the inhibition of MAPK, Akt, and NF-κB activation and the regulation of apoptosis-related protein expression. Furthermore, we found that PEP-1-GLRX1 was delivered to the SN via the blood–brain barrier (BBB) and reduced the loss of dopaminergic neurons in the MPTP-induced PD model. These results indicate that PEP-1-GLRX1 markedly inhibited the loss of dopaminergic neurons in MPP+- and MPTP-induced cytotoxicity, suggesting that this fusion protein may represent a novel therapeutic agent against PD.
Novel Bis- and Mono-Pyrrolo[2,3-d]pyrimidine and Purine Derivatives: Synthesis, Computational Analysis and Antiproliferative Evaluation
Authors: Bistrović Popov Andrea , Vianelo Robert , Grbčić Petra , Sedić Mirela , Pavelić Sandra Kraljević , Pavelić Krešimir , Raić-Malić Silvana
Published: June 2021

Novel symmetrical bis-pyrrolo[2,3-d]pyrimidines and bis-purines and their monomers were synthesized and evaluated for their antiproliferative activity in human lung adenocarcinoma (A549), cervical carcinoma (HeLa), ductal pancreatic adenocarcinoma (CFPAC-1) and metastatic colorectal adenocarcinoma (SW620) cells. The use of ultrasound irradiation as alternative energy input in Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) shortened the reaction time, increased the reaction efficiency and led to the formation of exclusively symmetric bis-heterocycles. DFT calculations showed that triazole formation is exceedingly exergonic and confirmed that the presence of Cu(I) ions is required to overcome high kinetic requirements and allow the reaction to proceed. The influence of various linkers and 6-substituted purine and regioisomeric 7-deazapurine on their cytostatic activity was revealed. Among all the evaluated compounds, the 4-chloropyrrolo[2,3-d]pyrimidine monomer 5f with 4,4′-bis(oxymethylene)biphenyl had the most pronounced, although not selective, growth-inhibitory effect on pancreatic adenocarcinoma (CFPAC-1) cells (IC50 = 0.79 µM). Annexin V assay results revealed that its strong growth inhibitory activity against CFPAC-1 cells could be associated with induction of apoptosis and primary necrosis. Further structural optimization of bis-chloropyrrolo[2,3-d]pyrimidine with aromatic linker is required to develop novel efficient and non-toxic agent against pancreatic cancer.
Lead-Free Cesium Titanium Bromide Double Perovskite Nanocrystals
Authors: Grandhi G. , Matuhina Anastasia , Liu Maning , Annurakshita Shambhavee , Ali-Löytty Harri , Bautista Godofredo , Vivo Paola
Published: June 2021

Double perovskites are a promising family of lead-free materials that not only replace lead but also enable new optoelectronic applications beyond photovoltaics. Recently, a titanium (Ti)-based vacancy-ordered double perovskite, Cs2TiBr6, has been reported as an example of truly sustainable and earth-abundant perovskite with controversial results in terms of photoluminescence and environmental stability. Our work looks at this material from a new perspective, i.e., at the nanoscale. We demonstrate the first colloidal synthesis of Cs2TiX6 nanocrystals (X = Br, Cl) and observe tunable morphology and size of the nanocrystals according to the set reaction temperature. The Cs2TiBr6 nanocrystals synthesized at 185 °C show a bandgap of 1.9 eV and are relatively stable up to 8 weeks in suspensions. However, they do not display notable photoluminescence. The centrosymmetric crystal structure of Cs2TiBr6 suggests that this material could enable third-harmonic generation (THG) responses. Indeed, we provide a clear evidence of THG signals detected by the THG microscopy technique. As only a few THG-active halide perovskite materials are known to date and they are all lead-based, our findings promote future research on Cs2TiBr6 as well as on other lead-free double perovskites, with stronger focus on currently unexplored nonlinear optical applications.
Oblique and Asymmetric Klein Tunneling across Smooth NP Junctions or NPN Junctions in 8-Pmmn Borophene
Authors: Kong Zhan , Li Jian , Zhang Yi , Zhang Shu-Hui , Zhu Jia-Ji
Published: June 2021

The tunneling of electrons and holes in quantum structures plays a crucial role in studying the transport properties of materials and the related devices. 8-Pmmn borophene is a new two-dimensional Dirac material that hosts tilted Dirac cone and chiral, anisotropic massless Dirac fermions. We adopt the transfer matrix method to investigate the Klein tunneling of massless fermions across the smooth NP junctions and NPN junctions of 8-Pmmn borophene. Like the sharp NP junctions of 8-Pmmn borophene, the tilted Dirac cones induce the oblique Klein tunneling. The angle of perfect transmission to the normal incidence is 20.4∘, a constant determined by the Hamiltonian of 8-Pmmn borophene. For the NPN junction, there are branches of the Klein tunneling in the phase diagram. We find that the asymmetric Klein tunneling is induced by the chirality and anisotropy of the carriers. Furthermore, we show the oscillation of electrical resistance related to the Klein tunneling in the NPN junctions. One may analyze the pattern of electrical resistance and verify the existence of asymmetric Klein tunneling experimentally.
Nucleation and Growth-Controlled Facile Fabrication of Gold Nanoporous Structures for Highly Sensitive Surface-Enhanced Raman Spectroscopy Applications
Authors: Lee Eunji , Ryu Sangwoo
Published: June 2021

The fabrication of porous metal structures usually involves complicated processes such as lithography or etching. In this study, a facile and clean method based on thermal evaporation at high pressure is introduced, by which a highly porous, black colored structure of Au can be formed through the control of homogeneous nucleation and growth during evaporation. The deposited films have different morphologies, from columnar to nanoporous structures, depending on the working pressure. These porous structures consist of Au nanoparticle aggregates, and a large number of nano-gaps are found among the nanoparticles. Thus, these structures indicate a much higher intensity of surface-enhanced Raman spectroscopy (SERS) when compared with commercial SERS substrates. The SERS intensity depends on the working pressure and thickness. Even circumstances that can induce agglomeration of nanoparticle aggregates do not deteriorate the sensitivity of SERS. These nanoporous structures based on high-pressure thermal evaporation are expected to provide a new platform for the development of low-cost and highly sensitive chemical sensors.
Tea Bags—Standard Materials for Testing Impacts of Nitrogen Addition on Litter Decomposition in Aquatic Ecosystems?
Authors: Mori Taiki
Published: June 2021

How the anthropogenic addition of nutrients, especially nitrogen (N), impacts litter decomposition has attracted extensive attention, but how environmental factors other than nutrients affect the impacts of N addition on litter decomposition is less understood. Since different local litters could respond differently to N addition, standard materials are necessary for comparing the impacts among various environments. The present study tested if tea bags used for the Tea Bag Index (TBI) approach, i.e., constructing an asymptote model by using a green tea decomposition datum and a rooibos tea decomposition datum (single measurement in time), can be standard materials for testing the impacts of N addition on litter decomposition in aquatic ecosystems. A laboratory incubation experiment was performed using a water sample taken from a stream in Kumamoto, Japan. Since a recent study suggested that the TBI approach may be inapplicable to aquatic ecosystems, a time-series data approach, i.e., fitting models to time-series mass loss data of tea bags, was also used for testing if tea bag decomposition can pick up the impacts of N addition on aquatic litter decomposition. The time-series data approach demonstrated that N addition significantly suppressed rooibos tea decomposition, whereas green tea decomposition was not affected by N addition. The TBI approach was unsuitable for testing the sensitivity of the response of tea bag decomposition to N addition because the TBI-based asymptote model failed to predict the observed data, confirming the suggestion by a previous study. Overall, the present study suggested that the tea bags can be used as standard materials for testing the impacts of N addition on litter decomposition in aquatic ecosystems, but only when using a time-series measurement and not the TBI.
Biogeographic Overview of Ulmaceae: Diversity, Distribution, Ecological Preferences, and Conservation Status
Authors: Fragnière Yann , Song Yi-Gang , Fazan Laurence , Manchester Steven R. , Garfì Giuseppe , Kozlowski Gregor
Published: June 2021

The elm family (Ulmaceae) is a woody plant group with important scientific, societal, and economic value. We aim to present the first biogeographic synthesis investigating the global diversity, distribution, ecological preferences, and the conservation status of Ulmaceae. A literature review was performed to explore the available data for all extant species. Our study made it possible to map the actual global distribution of Ulmaceae with high precision, and to elucidate the centers of diversity, located mainly in China and in the southeastern USA. A detailed comparative analysis of the macroclimatic niche for each species was produced, which shows the general biogeographic pattern of the family and pinpoints the outlier species. The results corroborate recent molecular analyses and support the division of Ulmaceae into two taxonomically, biogeographically, and ecologically well-differentiated groups: the so-called temperate clade with 4 genera and 43 species and the tropical clade with 3 genera and 13 species. The elm family is often described as a typical temperate plant group, however the diversity peak of all Ulmaceae is located in the subtropical zone, and a non-negligible part of the family is exclusively distributed in the tropics. We also noticed that a high proportion of Ulmaceae is linked to humid macro- or microhabitats. Finally, we highlighted that nearly 25% of all Ulmaceae are threatened. Fieldwork, conservation efforts, and research activities are still necessary for this family, particularly for the tropical members and the most endangered species.
Bacillus subtilis MBI600 Promotes Growth of Tomato Plants and Induces Systemic Resistance Contributing to the Control of Soilborne Pathogens
Authors: Samaras Anastasios , Roumeliotis Efstathios , Ntasiou Panagiota , Karaoglanidis George
Published: June 2021

Bacillus subtilis MBI600 (Bs MBI600) is a recently commercialized plant-growth-promoting rhizobacterium (PGPR). In this study, we investigated the effects of Bs MBI600 on the growth of tomato and its biocontrol efficacy against three main soilborne tomato pathogens (Rhizoctonia solani, Pythium ultimum, and Fusarium oxysporum f.sp. radicis-lycopersici-Forl). Furthermore, the root colonization ability of the Bs MBI600 strain on tomato roots was analyzed in vivo with a yellow fluorescence protein (yfp)-labeled strain, revealing strong colonization ability, which was affected by the root growth substrate. The application of Bs MBI600 on tomato plants resulted in significant increases in shoot and root lengths. Transcriptional activation of two auxin-related genes (SiPin6 and SiLax4) was observed. Single applications of Bs MBI600 on inoculated tomato plants with pathogens revealed satisfactory control efficacy compared to chemical treatment. Transcriptomic analysis of defense-related genes used as markers of the salicylic acid (SA) signaling pathway (PR-1A and GLUA) or jasmonic acid/ethylene (JA/ET) signaling pathway (CHI3, LOXD, and PAL) showed increased transcription patterns in tomato plants treated with Bs MBI600 or Forl. These results indicate the biochemical and molecular mechanisms that are activated after the application of Bs MBI600 on tomato plants and suggest that induction of systemic resistance (ISR) occurred.
The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the Hordeum vulgare Cytoplasm
Authors: Trubacheeva Nataliya V. , Divashuk Mikhail G. , Chernook Anastasiya G. , Belan Igor A. , Rosseeva Ludmila P. , Pershina Lidiya A.
Published: June 2021

The genetic mechanisms of fertility restoration in alloplasmic bread wheat with the barley cytoplasm are poorly explored. The effect of the 1BS chromosome arm on the fertility of bread wheat with the H. vulgare cytoplasm was studied depending on the incompleteness/completeness of the cytonuclear compatibility. (i) Three self-fertile (SF) lines and one partially fertile (PF) line with an incomplete cytonuclear compatibility and (ii) four self-fertile (SF) lines with a complete cytonuclear compatibility were studied. For the lines in group (i), the heteroplasmy (simultaneous presence of barley and wheat copies) of the 18S/5S mitochondrial (mt) repeat was revealed as well as the barley-type homoplasmy of chloroplast simple sequence repeats (cpSSRs). In the lines in group (ii), the 18S/5S mt repeat and cpSSRs were found in the wheat-type homoplasmic state. In all of the lines, the 1BS chromosome arm was substituted for the 1RS arm. The F1 plants of SF(i)-1BS × 1RS hybrids were fertile. The results of a segregation analysis in the F2 plants of SF(i)-1BS × 1RS showed that 1BS carries a single dominant fertility restorer gene (Rf) of bread wheat with the H. vulgare cytoplasm. All of the F1 plants of PF(i)-1BS × 1RS hybrids were sterile. A single dose of this restorer gene is not sufficient to restore fertility in this alloplasmic PF(i) line. All of the F1 and F2 plants of SF(ii)-1BS × 1RS hybrids were self-fertile.
Cortisol and Testosterone in Leadership Practice
Authors: Moe Helene Tronstad , Strand Martin Frank , Karp Tom , Norbom Hans Marius
Published: June 2021

This study sought to discover whether and how biological parameters can predict leadership behavior in the following leadership-related tasks: a face-to-face negotiation (Study 1), an individual problem-solving case (Study 2), and a group-based problem-solving case (Study 3). We replicated previous work by Mehta, Mor, Yap and Prasad in testing the dual-hormone hypothesis related to testosterone increase and cortisol decrease (Study 1), but our findings do not provide evidence to support the dual-hormone hypothesis. In Study 2, we found that high openness was a significant predictor in the individual problem-solving case. The results from Study 3 indicated that higher openness was related to a better score on the group exercise. Our findings did not support the dual-hormone model, and we did not find support for the seller-specific effect reported in Mehta et al. The original study included 64 participants with complete hormone data, while our replicational study involved 114 participants with complete hormone data.
The Necessity of Recycling of Waste Li-Ion Batteries Used in Electric Vehicles as Objects Posing a Threat to Human Health and the Environment
Authors: Sobianowska-Turek Agnieszka , Urbańska Weronika , Janicka Anna , Zawiślak Maciej , Matla Jędrzej
Published: June 2021

The automotive industry is one of the fastest-growing sectors of the modern economy. Growing customer expectations, implementing solutions related to electromobility, and increasingly stringent legal restrictions in the field of environmental protection, determine the development and introduction of innovative technologies in the field of car production. To power the most modern vehicles that include electric and hybrid cars, packages of various types of lithium-ion cells are used, the number of which is constantly growing. After use, these batteries, due to their complex chemical composition, constitute hazardous waste that is difficult to manage and must be recycled in modern technological lines. The article presents the morphological characteristics of the currently used types of Li-ion cells, and the threats to the safety of people and the environment that may occur in the event of improper use of Li-ion batteries and accumulators have been identified and described on the basis of the Regulation of the European Parliament and Council (EC) No. 1272/2008 of 16 December 2008 and No. 1907/2006 of 18 December 2006 on the classification, labeling and packaging of substances and mixtures and the registration, evaluation, authorization and restriction of chemicals (REACH), establishing the European Chemicals Agency.
Promising Pathway of Thermostable Mannitol Dehydrogenase (MtDH) from Caldicellulosiruptor hydrothermalis 108 for D-Mannitol Synthesis
Authors: Koko Marwa Y. F. , Sami Rokayya , Muhoza Bertrand , Khojah Ebtihal , Mansour Ahmed M. A.
Published: June 2021

In this study, we conducted the characterization and purification of the thermostable mannitol dehydrogenase (MtDH) from Caldicellulosiruptor hydrothermalis 108. Furthermore, a coupling-enzyme system was designed using (MtDH) from Caldicellulosiruptor hydrothermalis 108 and formate dehydrogenase (FDH) from Ogataea parapolymorpha. The biotransformation system was constructed using Escherichia coli whole cells. The purified enzyme native and subunit molecular masses were 76.7 and 38 kDa, respectively, demonstrating that the enzyme was a dimer. The purified and couple enzyme system results were as follows; the optimum pH for the reduction and the oxidation was 7.0 and 8.0, the optimum temperature was 60 °C, the enzyme activity was inhibited by EDTA and restored by zinc. Additionally, no activity was detected with NADPH and NADP. The purified enzyme showed high catalytic efficiency Kcat 385 s−1, Km 31.8 mM, and kcat/Km 12.1 mM−1 s−1 for D-fructose reduction. Moreover, the purified enzyme retained 80%, 75%, 60%, and 10% of its initial activity after 4 h at 55, 60, 65, and 75 °C, respectively. D-mannitol yield was achieved via HPLC. Escherichia coli are the efficient biotransformation mediator to produce D-mannitol (byproducts free) at high temperature and staple pH, resulting in a significant D-mannitol conversation (41 mg/mL) from 5% D-fructose.
Mental reactions of doctors against the background of the SARS-CoV-2 pandemic (message 3)
Authors: Kinkulkina Marina Arkadievna , Gardanova Zhanna Robertovna , Novikov Vladimir Vladimirovich , Khritinin Dmitriy Fedorovich , Khritinin Aleksandr Dmitrievich , Novikova Anna Mikhaylovna
Published: June 2021

This article continues the review of the complex of mental reactions of doctors against the background of the SARS-CoV-2 pandemic described in messages 1 and 2 in the context of possible psychotherapeutic and psychocorrective effects on doctors to prevent the negative impact of the pandemic conditions on their quality of life, adaptation and the presence/level of anxiety disorders and anosognosia.
Neuro-ophthalmology — history and present
Authors: Tropinskaya Olga Feliksovna
Published: June 2021

The article examines the stages of the origin and development of Russian neuro-ophthalmology. The modern achievements and priority directions of its development are presented.
The role of the nurse in providing predoctor medical care for gastric ulcer
Authors: GARANINA Lilia Andreevna , NAZAROV Artem Aleksandrovich
Published: June 2021

Treatment of diseases in the field of gastroenterology should be carried out taking into account the participation of many risk factors in the development of diseases. The modern rhythm of life, constant stress, malnutrition, unfavorable environmental conditions — all these affect the human body, including the gastrointestinal tract. The article describes the methods of providing pre-medical care for stomach ulcers.
Experience of the Pavlodar Regional Emergency Station in the context of the COVID-19 pandemic
Authors: Akhmetzhanov Kanadan Kanashevich
Published: June 2021

Due to the complicated epidemiological situation in the region, the number of calls to the emergency station has increased. In order to prevent a decrease in operational work, a number of changes were introduced which made it possible to reduce the time of arrival of the teams from the moment the call was received at the 103 console to 7 minutes and contributed to the provision of high-quality medical care to the population by emergency medical teams.
Semangat Reformasi Persembahan Umat Pasca Pembuangan Menurut Maleakhi 3:6-12
Authors: Panggabean Rudolf
Published: June 2021

The tithe offering to God shows the repentance of the people to Him. Obedience in giving a true tithe offering is a practice of covenant between God and His people, but n its implementation, people break their covenants against God's decree.  people still practice the wrong practices of worshipping God, especially regarding things. The real tithe is not of how much the people give to God, but rather a form of obedience to Him. This condition was conveyed by Malachi to the people of Israel. This study aims to analyze the text of Malachi 3:6-12 to gain an understanding of the spirit of reform of post-exile offerings. The method used in this study is qualitative by applying descriptive methods through the analysis of the social history of the text. In terms of the spirit of reform of the people after the exile according to the text of Malachi 4:6-12, it is obtained an understanding of the spirit of reform of the offering of the people as obedience through thanksgiving to God and to the common welfare.AbstrakPersembahan persepuluhan kepada Allah menunjukkan pertobatan umat kepada-Nya. Ketaatan dalam memberikan persembahan persepuluhan yang benar merupakan salah satu praktik perjanjian antara Allah dan umat-Nya, namun pada pelaksanaannya, umat melanggar perjanjian mereka terhadap ketetapan Allah itu. umat masih saja melakukan praktik peribadatan yang salah kepada Allah, khususnya mengenai persembahan perse-puluhan. Kondisi ini disampaikan Nabi Maleakhi kepada umat Israel. Penelitian ini bertujuan untuk menganalisa teks Maleakhi 3:6-12 untuk mendapatkan pemahaman semangat reformasi persembahan umat pasca pembuangan. Metode yang dipakai dalam penelitian ini adalah kualitatif, dengan menerapkan metode deskriptif melalui analisis sejarah sosial teks. Dalam hal semangat reformasi persmbahan umat pasca pembuangan  menurut teks Maleakhi 4:6-12, maka didapatkan pemahaman mengenai semangat reformasi persembahan umat sebagai ketaatan melalui ucapan syukur kepada Allah dan untuk kesejahteraan bersama
MODERN UNDERSTANDING OF INSTALLATION IN FORENSIC TECHNICAL EXAMINATION OF DOCUMENTS
Authors: Kulykovska V.
Published: June 2021

The article presents an attempt to provide a general methodological concept of document mounting and describe its main methods and features.
The article considers practical examples in which a scheme-algorithm for conducting research is described and a logical chain of established facts is built, which helped in each case to come to a reasoned conclusion.
The article also draws attention to the fact that today the schemes for solving problems to establish the execution of documents using installation are the least developed both theoretically and scientifically-methodically - there is no single terminological approach that allows you to unambiguously interpret and evaluate the results obtained.
FEATURES OF CONDUCTING RESEARCH ON CASES RELATED TO THE ACCRUAL AND PAYMENT OF AVERAGE EARNINGS FOR THE PERIOD OF FORCED ABSENTEEISM IN THE CASE OF THE ILLEGAL DISMISSAL OF AN EMPLOYEE
Authors: Kandiral O.
Published: June 2021

The article discusses the features of research on the validity of the calculation and payment of average earnings for the period of forced absenteeism in the case of illegal dismissal of an employee. For the purpose of this scientific study, it is the formation of a unified approach in conducting forensic economic examinations in cases related to the calculation and payment of average earnings for the period of forced absence for the illegal dismissal of an employee.
The article systematizes the regulations governing labor relations in Ukraine. The advantages and disadvantages of applying the current labor legislation in practice are described and analyzed. The author points out the need to concretize and generalize legal norms in order to implement the principle of legal certainty, modernize outdated provisions of the Labor Code of Ukraine in order to take into account the labor legal relations that currently exist, business customs and avoid possible abuse of their rights by both the employer and the employee.
The urgency of this problem and its impact on the growth of the national economy are noted. The questions of the search for the solution of the problems facing the forensic economic examination regarding the accrual and payment of average earnings for the period of forced absence from the illegal dismissal of an employee are raised.
The article systematizes and summarizes the forensic and expert practice, which made it possible to draw conclusions about the ambiguous interpretation of the law, which in turn leads to disputes between the employer and the unlawfully dismissed. Then reinstated, hired employee regarding the accrual and payment of average earnings for the period of forced absenteeism upon his/her illegal dismissal.
The author sets out the methodological principles of conducting forensic examinations in cases related to the calculation and payment of average earnings for the period of forced absence from the illegal dismissal of an employee, the subject, tasks and objects of the examinations are determined.
The result of the work done, the author proposed an algorithm for the sequential actions of an expert during forensic economic research on the validity of calculating and paying average earnings for the period of forced absenteeism in the event of illegal dismissal of an employee.
The results of the article can be used by judicial and law enforcement agencies during the investigation and consideration of cases, the subject of which is the establishment of facts of compliance by employers with the requirements of the current labor legislation, in expert practice, during scientific and methodological work, as well as in further scientific developments on these issues.
GENERAL ISSUES OF FORENSIC CHARACTERIZATION OF MONEY LAUNDERING
Authors: Dumchykov M. , Bondarenko O. , Utkina M.
Published: June 2021

The purpose of the article is to analyze approaches to the formation of the essence and constituent elements of the forensic characteristics of the legalization of corruption proceeds (Article 209 of the Criminal Code of Ukraine), to highlight and describe the elements of the forensic characteristics of this criminal offense.
Forensic characteristics play a special role in the very structure of the methodology for investigating a criminal offense of this type. A successful and comprehensive study of the circumstances of a criminal offense largely depends not only on the correct definition of the criminal-legal signs of an act, but also on the investigator’s understanding of the criminalistics nature of the corresponding offense.
The analysis of publications indicates the presence of a number of disagreements between the authors in the construction of the forensic characteristics of the legalization (laundering) of proceeds from crime.
Comparison of the existing doctrinal approaches to understanding the structural elements of the forensic characteristics of the legalization of funds obtained by criminal means, made it possible to form the author’s approach. According to the authors of the article, the forensic characteristics of the legalization (laundering) of incomes obtained as a result of the commission of a criminal offense should include both basic and additional elements. The main elements of the forensic characteristics of legalization are proposed to include the following: typical traces of a criminal offense; its subject; the way of committing such an offense.
Additional, according to the authors of the article, it is advisable to include the following: the subject of a criminal offense; the time of its commission; the area in which the offense is committed; the environment of the criminal offense; source of origin of “dirty money”.
The proposed elements of the forensic characteristics of a criminal offense can be the starting point in the development of an effective methodology for investigating money laundering.
HISTORICAL PERIODIZATION (HISTORICAL AND CHRONOLOGICAL ASPECT) OF "INVESTIGATION METHODS" OF CRIMINAL OFFENSES
Authors: Starushkevych А.
Published: June 2021

Methodological problems of historical periodization are considered, in particular, the historical and chronological aspect of the "methods of investigation" of criminal offenses. It is concluded that in order to study the process of the emergence, formation and development of "investigation methods", it is advisable to develop a historical periodization, which would provide for the division of such a process into segments (periods, stages, stages) reflecting the qualitative state of the object under study.
It is not enough to consider the process of formation and development of investigation methods as "biography", that is, through the work of individual scientists or scientific schools, it is more successful and profound to study the origins, prerequisites, the process of formation and development of the subject under study using elements of the activity approach, taking into account changes in needs , subjects, conditions, means, algorithms of actions on individual subjects studied (in our case, "methods of investigation") at certain variable time and local intervals. And also taking into account the general historical and cultural contexts, combining with the principles of "stadiality", "polyvariance" and "civilizational discreteness" generally recognized in historical science.
The periodization of the emergence, formation and development of "investigation methods" must meet certain requirements: 1) rely on common principles for dividing the time tape, taking into account the object and subject of research, its goals and objectives; 2) the criteria for identifying periods must be scientifically grounded and take into account the periodization used in science (in particular, historical); 3) periodization should have as clear boundaries as possible, which are usually marked by events that were most important in the historical process; 4) the criteria of division, which became the basis of periodization, do not exhaust all the tendencies and patterns of the investigated "methods of investigation", therefore, such periodization usually has certain cognitive limitations, and this fact must be taken into account.
The study of the historical aspect of "methods of investigation" should involve two main approaches. The first one is to consider the process of development of "methods of investigation" and an idea of them in the classical periods of history: prehistoricity, antiquity, the Middle Ages, early modern times, modern times, modern times, taking into account the elements of "total" and "local" periodization. The second approach provides for the identification of the stages of the emergence and development of "investigation methods", taking into account the degree of development of forensic knowledge, as well as the formation of forensic science as an "organized science" (the formation of its sections, in particular forensic methods, the emergence of forensic scientific schools, the differentiation of science into separate theories and teachings and the like).
Oferta de assistência nutricional a crianças menores de cinco anos na Estratégia de Saúde da Família
Authors: Paula Weslla Karla Albuquerque Silva de , Silva Bianca Santana , Oliveira Horasa Gonçalves Leal de , Lavôr Priscila Monteiro Tavares de , Samico Isabella Chagas
Published: June 2021

OBJETIVO: Avaliar a oferta da assistência nutricional às crianças menores de cinco anos assistidas pelas equipes da Estratégia Saúde da Família. MÉTODO: Estudo transversal, avaliativo, ancorado no modelo sistêmico de Donabedian, sendo avaliadas as dimensões estrutura e processo. Perfizeram a amostra 41 enfermeiros das equipes da Estratégia de Saúde da Família de um município do interior de Pernambuco. RESULTADOS: Em relação a estrutura, o item recursos humanos foi o único classificado como regular e os demais aspectos foram pontuados como bons.  Quanto ao componente processo, os itens avaliados apresentaram conformidade, sendo classificados entre bom e excelente. CONCLUSÃO: Evidenciou-se a necessidade da formação continuada dos enfermeiros das equipes de saúde da família para o desenvolvimento das ações de alimentação e nutrição.
Depressão entre idosos portadores de doença de Parkinson: opinião dos membros da Associação Capixaba de Parkinson
Authors: Fernandes Hellen Cristina Oliveira , Scarpat Lìvia Tassinari , Carvalho Marina Miguel de , Oliveira Núbia Dutra de , Salim Rebeca Netto Habib , Souza Rodolpho Rodrigues de , Madeira Elizabeth Santos
Published: June 2021

INTRODUÇÃO - A Doença de Parkinson é a segunda enfermidade neurodegenerativa mais comum no idoso, enquanto a depressão é uma síndrome com transtorno do humor. Os sintomas depressivos são as manifestações não motoras mais comuns entre os acometidos pela Doença de Parkinson. OBJETIVO - Caracterizar as mudanças ocorridas nos hábitos de vida dos acometidos pelo Parkinson, identificar a percepção dos membros da Associação sobre os sintomas de depressão, identificar a percepção dos mesmos sobre a relação entre Doença de Parkinson e depressão e caracterizar o seu convívio social com a depressão. METODOLOGIA - Estudo descritivo qualitativo, no qual foram entrevistados membros idosos da Associação Capixaba de Parkinson. As entrevistas foram gravadas, transcritas, categorizadas, armazenadas e submetidas a análise de discurso. Este estudo foi aprovado pelo Comitê de Ética da Faculdade Brasileira- Multivix sobre número 47289215.1.10000.5066. RESULTADOS - O estudo revela que os diferentes quadros clínicos do Parkinson são definidores dos hábitos de vida desses pacientes, já que causam limitação motora, transtornos do sono e memória, perda da autonomia e desmotivação. Na visão dos membros da Associação Capixaba de Parkinson, os sintomas depressivos são reconhecidos pela maioria dos portadores de Parkinson, que acreditam que há relação entre ambas as doenças. Dessa forma, indivíduos diagnosticados com as duas enfermidades podem ter alterações em seu convívio social, como o isolamento e a busca por grupos de apoio. CONCLUSÃO - O Parkinson gera prejuízos em vários âmbitos da vida do portador, principalmente quando está associado a depressão. Os parkinsonianos reconhecem os sintomas depressivos e percebem a relação com a Doença de Parkinson. Aspectos físicos e mentais devem ser levados em consideração no tratamento da doença, necessitando de uma abordagem multiprofissional e interdisciplinar.
Avaliação da cadeia de frio do transporte de vacina: estudo transversal em Minas Gerais, Brasil
Authors: Oliveira Valéria Conceição de
Published: June 2021

Estudo transversal de abordagem avaliativa do grau de implantação da cadeia de frio durante o transporte de vacina do nível regional para municípios. Avaliamos 53 rotas de transporte de vacina de municípios de Minas Gerais. Para a coleta de dados, utilizamos um questionário estruturado e o monitoramento das temperaturas das caixas de vacina encaminhadas aos municípios através do data logger. O grau de implantação foi definido por meio de um sistema de escores, com pesos diferenciados para indicadores em cada dimensão avaliada. Os escores obtidos a partir da soma dos pontos dos indicadores foram transformados em percentuais, com referência à pontuação máxima possível. A seguir, foram definidas as categorias para o grau de qualidade: “adequado”, “parcialmente adequado” e “não adequado”. Os dados foram digitados no programa Epidata 3,0 e analisados no EPI-INFO 7.0, GraphPad Prism 5 e BioEstat 5.0, Excel. Realizamos análise univariada com cálculo das frequências, medidas de aferição e dispersão. A cadeia de frio durante o transporte de vacina não está adequadamente implantada na maioria dos municípios avaliados. Em vinte e dois municípios as vacinas foram expostas a temperaturas abaixo de +2ºC, sendo que em seis destes a temperatura variou de 0oC a +0,5ºC. O transporte de vacina apresenta falhas que podem comprometer a qualidade dos imunobiológicos.
Identificação de infecções do trato reprodutivo em mulheres atendidas na Atenção Primária à Saúde
Authors: Gomes Liliane Soares , Holanda Viviane Rolim de , Barros Mariana Boulitreau Siqueira Campos
Published: June 2021

Objetivo: identificar a frequência das infecções do trato reprodutivo em mulheres atendidas em uma unidade de atenção primária à saúde, na região da zona da mata de Pernambuco. Método: Trata-se de um estudo retrospectivo, documental, de corte transversal, realizado com 361 registros de mulheres na faixa etária dos 14 aos 87 anos. Para a análise, procedeu-se a distribuição das frequências absolutas e relativas das variáveis coletadas, assim como medidas de tendência central e de dispersão, e representados por tabelas através do programa estatístico EpiInfo versão 7.1.5.2.Pesquisa aprovada pelo Comitê de Ética em Pesquisa, com CAAE nº 54417916.2.0000.5208. Resultados: A Gardnerella vaginalis (16,62%) foi o agente microbiológico mais frequente que os demais causadores de infecções do trato reprodutivo; os microrganismos foram mais prevalentes na faixa etária de 25-34 anos. Discussão: a equipe de saúde deve estar atenta às orientações relacionadas à vaginose bacteriana, uma vez que esta se configurou como a mais prevalente afecção nas mulheres usuárias do serviço, considerando o tratamento empregado após o diagnóstico microbiológico. O elevado número de registros de mulheres jovens deve-se ao maior cuidado com a saúde íntima, pois se sugere que estas se encontram em pleno exercício da atividade sexual, e tem maior acesso a informação e aos serviços de saúde. Conclusão: Sob o ponto de vista dos benefícios do cuidado à saúde da mulher, na atenção primária, incentiva-se a atuação profissional no sentido de promover ações de educação em saúde visando a conscientização de mulheres das diversas faixas etárias, sobre a relevância das ITR’s.
Reflexões sobre as diretrizes dos Núcleos de Apoio à Saúde da Família acerca do profissional fisioterapeuta
Authors: Bim Cíntia Raquel , González Alberto Durán
Published: June 2021

No âmbito do Sistema Único de Saúde - SUS, ainda não existe uma política pública de saúde consolidada sobre o papel da fisioterapia, em particular na atenção básica. O perfil epidemiológico e a transição demográfica brasileira levam a fisioterapia a rever sua práxis profissional e adequar suas ações para a atenção básica, visando maior integralidade e resolubilidade dos problemas de saúde da população neste nível de atenção.  Os Núcleos de Apoio à Saúde da Família - NASF criados em 2008 são a maior oportunidade de inserção do fisioterapeuta na atenção básica. O objetivo deste trabalho foi realizar uma reflexão crítica do capítulo direcionado a fisioterapeutas nas diretrizes do NASF, de 2009. O texto apresenta-se confuso sobre a atuação deste profissional na atenção básica, e ainda direciona muito suas práticas à reabilitação. Considera o fisioterapeuta como profissional de reabilitação para atuar com idosos/deficientes, o que é um contra censo ao que se preconiza como ações para a atenção primária em saúde, tanto pela restrição às ações de reabilitação, quanto por colocar apenas duas condições de saúde como aptas à atuação da fisioterapia. O documento traz direcionamentos para atuação do fisioterapeuta apenas para idosos e deficientes, não abrangendo os problemas de saúde de uma população considerando todos os problemas de saúde que uma população pode apresentar. A apresentação do conteúdo destinado a fisioterapeutas difere da apresentação de outros profissionais da saúde que também atuam no NASF, tendo estes direcionamentos mais específicos. Conclui-se que a referida política não fortalece a atual demanda da população sobre as práticas do fisioterapeuta na atenção básica à saúde, tão pouco auxilia na quebra de paradigma do fisioterapeuta como profissional exclusivo da reabilitação. São necessárias novas políticas públicas de saúde voltadas para o trabalho do fisioterapeuta na atenção básica para que este profissional possa ampliar suas ações neste nível de atenção.
O trabalho em equipe multiprofissional na Residência: a perspectiva dos residentes multiprofissionais
Authors: Assunção Natália Gualberto , Martins Lívia Módolo
Published: June 2021

Mudanças no contexto das políticas públicas desencadearam transformações nas práticas de atenção em saúde por meio da reorganização do sistema de saúde e formação de profissionais para atuação no setor. A Residência apresenta-se como uma das ferramentas para formação de profissionais de saúde, enfatizando cuidado integral e trabalho em equipe. O objetivo da pesquisa foi analisar a percepção de residentes multiprofissionais sobre trabalho em equipe na APS; e apreender suas perspectivas acerca de sua inserção nas equipes de APS, na Residência. Utilizou-se análise de conteúdo dedutiva (Bardin, 2010), que é uma ferramenta para análise de dados que visa interpretar um material de caráter qualitativo, descrevendo o conteúdo manifesto nas comunicações. Realizaram-se entrevistas semiestruturadas com quatorze residentes, que atuam em Unidades de Saúde da Família, de Ribeirão Preto, São Paulo. Definiram-se quatro categorias temáticas para análise e discussão. Foram realizados apontamentos sobre formação na graduação e na Residência Multiprofissional para o trabalho em equipe na APS; reflexões sobre concepções e desafios do trabalho em equipe, e da inserção desses residentes nas equipes de APS, na Residência. Evidenciou-se que os residentes apresentam visão ampla sobre o trabalho em equipe, especialmente acerca do cuidado integral dos usuários, contemplando os profissionais, que se beneficiam da troca de experiência proporcionada por ações conjuntas. A Residência tem sido cenário importante de formação para profissionais de saúde, propiciando trocas e vivências práticas conjuntas. A pesquisa também propiciou espaço para reflexão sobre própria atuação e movimentos que podem ser dados visando melhorias no trabalho em equipe na APS.
Planejamento estratégico na Atenção Primária em Saúde
Authors: Calvo Amanda Ramos Berti Guilhen , Bracciali Luzmarina Aparecida Doreto , Caleman Gilson , Marin Maria José Sanches , Nascimento Edinalva Neves
Published: June 2021

Objetivo: Analisar a construção de um projeto de intervenção com os gestores municipais de saúde e suas equipes, a partir de um problema relevante para eles. Método: Utilizou-se a ferramenta de gestão do PES, proposta por Carlos Matus, com os momentos: explicativo, normativo, estratégico e tático-operacional. Participaram 32 trabalhadores da Atenção Primária a Saúde. A coleta de dados foi realizada durante 12 encontros quinzenais nos meses de dezembro de 2017 a maio de 2018. Resultados e Discussão: Priorizados pelos participantes o problema: falta de organização do processo de trabalho na Atenção Primária a Saúde; os descritores: Descritor 1: 90% dos servidores municipais não tem capacitação para trabalhar na atenção primária à saúde; Nó crítico1: não existe um espaço coletivo de discussão de problemas e ações; Intervenção: criação de um espaço em cada equipe da APS para discussão dos problemas por meio da Educação Permanente em Saúde e Descritor 2: não aplicação/ elaboração de protocolos que orientem os serviços, sobretudo na unidade; Nó crítico 2: não existência de protocolos na atenção primária à saúde; Intervenção: Disponibilizar recursos para os profissionais se apropriarem dos conceitos e práticas para construção de protocolos. Considerações Finais: Gestores e equipes construíram o planejamento utilizando a ferramenta do PES com momentos de divergências de ideias, porém, houve fortalecimento do vínculo, tanto no processo de trabalho, como na convivência e na construção de intervenções.