bluuebunny/crossref_metadata_2025 huggingface dataset:
Peran Manajemen Pendidikan di Era Digital
Authors: Khoiri Ahmad , Wulan Werdining
Published: January 2025

Tujuan manajemen pendidikan di era digital adalah memastikan pendidikan berkualitas tinggi, tepat guna, bertanggung jawab, dan berdampak positif bagi masyarakat. Penelitian ini bertujuan untuk meningkatkan pemahaman tentang manajemen yang diperlukan untuk organisasi pendidikan. Peneliti menggunakan pendekatan deskriptif kualitatif melalui studi kepustakaan atau studi kepustakaan. Selanjutnya, data yang dikumpulkan dianalisis menggunakan pendekatan deskriptif-analistis; proses ini melibatkan penelaahan dan evaluasi. Pada kenyataannya, Indonesia masih perlu mengubah infrastruktur informasi teknologinya hingga saat ini. Masalah saat ini tidak dapat diselesaikan dengan cara yang sama seperti konsep sebelumnya. Manajemen adalah komponen penting dalam organisasi yang mengelola kehidupan agar berjalan lancar. Di era digital, perlu meningkatkan kualitas pendidikan. Ini termasuk meningkatkan kemampuan siswa untuk belajar dan mendukung program belajar bebas. Menejemen pendidikan menghadapi banyak masalah, seperti pendidik yang tidak terampil dalam menghadapi era digital dan ancaman keamanan seperti peretasan data.
Implementasi EDM dan E-RKAM di Madrasah Ibtidaiyah Muhammadiyah Tanjungsari
Authors: Berlianto Abdul Fitri , Wulandari Laila
Published: January 2025

This study aims to get an overview of the implementation of EDM and E-RKAM in Madrasah Ibtidaiyah Muhammadiyah Tanjungsari Banyudono. This study uses a qualitative method where all information obtained from observations, documents and interviews is described in the form of written narratives as findings in the field. The results of the study illustrate that the implementation of EDM and E-RKAM is going well. The stages passed are also in accordance with the guidelines from the Ministry of Religion of the Republic of Indonesia, namely forming a TEAM, conducting socialization, collecting data and information, discussing and setting the level of each indicator, filling out instruments available online or semi-online, the Madrasah Head approves the results of the EDM fields, send EDM filling results. The results of the EDM will be used as material for determining priority types of programs/activities in preparing plans for the improvement and development of madrasas as outlined in the E-RKAM. The benefits of implementing EDM and E-RKAM include: knowing the strengths, weaknesses and challenges that madrasas have, knowing opportunities to improve the quality of education, knowing the types of needs needed to improve the quality of education, knowing the level of achievement of madrasah performance, being able to identify priority programs/activities for improving madrasah performance.
Optimalisasi Manajemen Humas dalam Meningkatkan Kompetensi Guru dan Siswa melalui Kerja Sama Eksternal
Authors: Faozi Ahmad , Bakhri Syaiful
Published: January 2025

This research is motivated by the high unemployment rate of vocational high school graduates which is one of the main problems in the world of vocational education in Indonesia. This is inseparable from the incompatibility of graduate competencies with industry needs. In addition, cooperation with industry that is not optimal and the inequality in the number of cooperation between departments also worsens the situation. In this condition, competition between vocational schools has become increasingly difficult to force each school to continue to improve the quality of its education. This study uses a descriptive qualitative method with a case study approach. Data collection is carried out through interviews, observations, and documentation. After that, the data is analyzed by reducing, presenting, and drawing conclusions. The results of the first planning research set the goals of the cooperation to be carried out. Second, update information, make an MoU, and make a timeline for coordination meetings and report results. The implementation of public relations in building the first cooperation of public relations carries out cooperation with industry in an effort to improve teacher competence through training and student competence through street vendors and training. Second, public relations in updating information through offline and online. The three public relations agencies have implemented many MoUs, there are 18 MoUs carried out by public relations. Fourth, in the implementation of coordination meetings with school structures, they are carried out every Monday, while internal public relations meetings are held once a month. The first evaluation is to re-check that the cooperation carried out is in accordance with the vision and mission, the second in evaluating the update of public relations information involves departments to participate in evaluating. Third, the evaluation of the MoU is carried out when the cooperation period has expired, which is every two years. The four evaluation techniques used by public relations are two, namely oral techniques and written techniques. The impact of the collaboration with external parties is an increase in teacher competence through the internship program. Students benefit from Field Work Practice (PKL) programs that are relevant to industry needs. Further research could focus on the direct evaluation of the impact of cooperation with industry on job skills and the level of graduate absorption in the job market.
Guinea Pig Breeding, Consumption, Prices, and Exports in Peru: What Do the Statistical Sources Say?
Authors: Leiva Cárdenas Favio
Published: January 2025

This article aims to compile and estimate the stock, breeding, and consumption of guinea pigs in Peru from a long-term perspective, using data from agricultural censuses and a market perspective based on household surveys, agricultural surveys, and export data. The objective is to provide a quantitative and market-oriented approach to the current situation of guinea pig breeding and consumption in Peru. The guinea pig is an important source of protein and holds significant historical and cultural value, as it was one of the first animals domesticated in pre-Hispanic times. The results reveal a lack of diversification in export markets, which are primarily concentrated in the United States. Additionally, it is revealed that companies exporting guinea pigs are often multiproduct exporters for which guinea pig is not the main product but a complementary one. Despite the significant price increases in the Peruvian agricultural sector, it is not evident that breeders directly benefit from this rise due to the high level of self-consumption. As regards sales, the main places where consumers buy guinea pigs are retail markets and street vendors. Therefore, a strategy focused on selling large quantities of guinea pigs should consider retail markets, while a strategy targeting higher prices but lower volumes could focus on exports and sales to restaurants, which have higher prices but lower sales volumes in kilograms. This study also finds that sales of guinea pigs are more regular than self-consumption. The main contribution of this article is to offer different estimates of guinea pig sales, consumption, and stock based on agricultural censuses, agricultural surveys, and household surveys, opening up the possibility for similar research on other important animal stocks. This can allow entre­preneurs, researchers, and policymakers to make more realistic projections and ground-based strategies to generate value and promote welfare. Under­standing the sales and consumption patterns of these animals will help improve productivity policies and market integration for those engaged in livestock breeding.
Produção de mudas de amburana cearensis (allemao) a.c smith em diferentes substratos irrigada com efluente industrial
Authors:   , Alves Lívia Laiane Barbosa
Published: January 2025

Apesar de abundante no planeta terra, nas últimas décadas os problemas relacionados a água
acabaram sendo intensificados, principalmente nas regiões semiáridas. Diante da necessidade
de se produzir mudas para diversas finalidade, a qualidade da água para irrigação das plantas
em alguns casos pode comprometer a produção, assim uma das principais alternativas para
suprir a demanda nesses locais é o reúso de água. Objetivou-se com este trabalho foi avaliar o
crescimento da espécie Amburana cearensis em diferentes substratos utilizando efluente de
uma indústria de produtos de limpeza. O estudo foi desenvolvido em casa de vegetação no
Departamento de Ciências Agronômicas e Florestais, da Universidade Federal Rural do
Semiárido (UFERSA), Mossoró-RN. O experimento foi conduzido em blocos casualizados, em
esquema fatorial 2 x 5, correspondente a dois substratos e cinco doses do efluente (0%, 25%,
50%, 75% e 100%). Os substratos utilizados foram constituídos de: solo e composto orgânico
(S1); solo e fibra de aguapés (S2). Realizou-se análises químicas e de metais tóxicos no
efluente antes do início do experimento. Para os substratos as mesmas análises foram
realizadas antes do início e após o fim do experimento. O crescimento foi avaliado por meio
do diâmetro do caule (DC) e altura da muda (H), e as avaliações foram realizadas a cada 15
dias. Foram determinadas as taxas de crescimento absoluto (TCA) e taxas de crescimento
relativo (TCR). Os resultados das características avaliadas foram submetidos a análise de
variância e para os fatores qualitativos foi feita análise de média pelo teste de Tukey a 5% de
probabilidade. Para os fatores quantitativos, foi realizada análise de regressão e correlação.
Verificou-se que o diâmetro do caule médio foi maior para as plantas cultivadas em S2 e a
altura média foi maior em S1. A TCR para altura de planta e para diâmetro do caule
apresentaram o mesmo padrão de comportamento no tratamento testemunha, e nas doses 25 e
50% em S1. Em S2, a TCR para diâmetro reduziu de forma contínua durante os 90 dias em
todas as doses. As taxas de crescimento relativo para altura apresentaram uma tendência de
redução independente dos tratamentos. O pH e a condutividade elétrica foram mais elevados
em S2. Os metais tóxicos foram registrados em baixas concentrações nos substratos e nas
plantas. Dessa forma, as mudas apresentaram boas característica de crescimento para todos os
tratamentos, sendo que a dose 50% em S1 atingiu os melhores resultados para altura e
diâmetro.
Condicionamento fisiológico e tolerância ao estresse salino em sementes de coentro (coriandrum sativum l.)
Authors:   , Dantas Nadjamara Bandeira de Lima
Published: January 2025

O coentro (Coriandrum sativum L.) é amplamente cultivado no nordeste brasileiro, principalmente pela agricultura familiar, no entanto, a salinização dos solos e das águas constitui um dos principais problemas ambientais enfrentados nesta região, podendo comprometer o seu desenvolvimento. Neste sentido, o condicionamento fisiológico pode ser uma alternativa promissora para minimizar tais efeitos negativos durante a germinação e crescimento inicial de plântulas. Sendo assim, objetivou-se avaliar métodos para o condicionamento fisiológico em sementes de coentro, e seus efeitos na germinação e vigor em condições de salinidade. Para isto, utilizaram-se três lotes de sementes de coentro, cultivar SF Verdão 177, adquiridos no comércio local. O estudo foi dividido em duas etapas, sendo inicialmente avaliada a qualidade inicial dos lotes através da determinação do grau de umidade, primeira contagem, germinação, comprimento de plântulas, massa seca de plântulas, envelhecimento acelerado e condutividade elétrica. Os métodos testados para o condicionamento foram: hidrocondicionamento por 1, 2 e 3 dias; osmocondicionamento em solução de polietilenoglicol (PEG) 6000, nas concentrações de -0,2 e -0,4 MPa por 1, 2 e 3 dias e a testemunha. O delineamento experimental foi o inteiramente casualizado (DIC), com quatro repetições de 25 sementes. Na segunda etapa, sementes condicionadas e não condicionadas foram colocadas para germinar sob estresse salino nos níveis 2, 4, 6 e 8 dS.m-1 a 20-30 °C. O delineamento experimental foi o DIC, em esquema fatorial 2 x 4 (sementes condicionadas e sem condicionamento e quatro níveis de salinidade), com quatro repetições de 25 sementes. As variáveis analisadas em ambas as etapas foram: primeira contagem, germinação, comprimento de plântulas e massa seca de plântulas. Verificou-se que osmocondicionamento com solução de PEG 6000 -0,2 MPa é benéfico no desempenho de sementes de coentro. No entanto, os lotes responderam diferentemente com relação ao tempo de condicionamento, sendo recomendado o período de 1, 2 e 3 dias para os lotes B, C e A, respectivamente. O condicionamento osmótico favoreceu a germinação e vigor das sementes sob estresse salino.
Surgical Advancements, Immunotherapy, Targeted and Conventional Therapies, Biopsy, Colposcopy, and Pap Smear Integration in the Management of Cervical Cancer
Authors: Wargantiwar Siddhi , Bhattacharya Sankha , Kanugo Abhishek
Published: January 2025

Cervical cancer remains a significant global health concern, making it essential
to investigate new treatment options continuously. This page provides an overview
of the latest advancements and best practices in detection and intervention, including
Pap smears, colposcopy, biopsy, immunotherapy, targeted therapies, chemotherapy, radiation
therapy, and surgery. Surgical techniques such as radical hysterectomy and minimally
invasive procedures have advanced to enhance patient outcomes and quality of
life. Simultaneously, radiation therapy methods have been refined to maximize tumour
control while reducing adverse effects. Chemotherapy remains vital, with new drugs and
combination regimens demonstrating improved tolerance and efficacy. Immunotherapy,
notably immune checkpoint inhibitors, has shown promise in advanced stages of cervical
cancer. Additionally, targeted therapies that focus on specific biochemical pathways
offer the potential for personalized treatment approaches. This review critically assesses
ongoing research, evaluates existing data, and emphasizes the opportunities and challenges
of each therapeutic approach. Ultimately, integrating these diverse treatment
strategies is the key to enhancing patient outcomes.
The Role of Sirtuins in Diabetic Nephropathy: A Comprehensive Review
Authors: Wal Pranay , Tarannum Tarannum , Baldaniya Lalji , Kaur Kiranjeet , Singh Priyanka , Aziz Namra , Singh Komal , Gasmi Amin
Published: January 2025

Introduction:

Diabetic nephropathy is characterized by elevated oxidative stress and
chronic inflammation in the kidneys. A class of proteins called sirtuins is well-known to be important
for a number of cellular functions, such as metabolism, stress tolerance, and ageing. Among
them, SIRT1 is associated with the progression of diabetic nephropathy, a dangerous kidney-related
consequence of diabetes mellitus. Thus, this study aims to examine the function and pathways
of sirtuin that are responsible for the progression of this disease.

Methods:

Publications considered from the standard databases like PUBMED-MEDLINE, Google
Scholar, and Scopus using standard keywords, “Sirtuin,” Signalling pathway”, and “Diabetic
Nephropathy” well described the actual knowledge on the scientific literature indicating patient
susceptibility to kidney disease that is influenced by sirtuin-1 gene variants.

Results:

The research results imply that sirtuins offer enormous promise as cutting-edge therapeutic
targets for kidney disease prevention and management. Renal fibrosis, metabolic disorders, renal
impairment, and a possible regulation mechanism all probably entail blocking inflammation
through various signalling pathways.

Conclusion:

A comprehensive understanding of the fundamental pathophysiological pathways targeting
sirtuin is essential as a diagnostic tool. For the treatment of diabetic nephropathy, researchers
are developing therapeutic techniques to target biological roles and functions of different types
of sirtuin, processes, and signalling pathways.
Sortilin as a Culprit in the Atherosclerosis Plaque Progression:
Evidence from Clinical and Experimental Studies
Authors: Cheng Guangyu , Liu Jie , Zhang HongLi , Cui YouXiang , Xu ShuJie , Wang Lichun
Published: January 2025

Sortilin acts as a key receptor for lipids, growth factors, cytokines, and
enzymes and participates in pathological cargo loading and transferring of extracellular
vesicles. Emerging evidence suggests a significant role of sortilin in hyperlipidemia
and the risk of atherosclerosis. Recent epidemiological evidence demonstrated that
sortilin has been implicated in atherosclerosis plaque progression in patients with
coronary or peripheral artery disease. The present study presents a comprehensive
review of the contribution of sortilin to atherosclerosis progression. Here, recent
experimental and clinical findings are summarized to determine the effects of sortilin
on atherosclerosis progression and the related underlying mechanisms.
Targeting Glutamine Metabolic Reprogramming in Pancreatic Cancer: Current Insights and Future Directions
Authors: Ma Yanhui , Wang Mingling , Zhang Kexing , Ren Fangbing , Wang Yuqun , Zhang Wenqiang , Kan Chengxia , Han Fang , Sun Hongxi , Sun Xiaodong
Published: January 2025

Pancreatic Cancer (PC) is a devastating malignancy with a poor prognosis and in-creasing morbidity. Current treatment strategies have limited efficacy in improving patient survival. Metabolic reprogramming is a hallmark of cancer and plays a key role in the pro-gression and maintenance of PC. PC cells exhibit a unique glutamine metabolism that is dis-tinct from other cancer types. The non-classical pathway of glutamine metabolic reprogram-ming plays a “permissive role” in the survival and proliferation of PC cells, mainly by affect-ing the redox homeostasis of the cells. In this review, we compare and contrast the canonical and non-canonical glutamine metabolic pathways and highlight recent advances in targeting non-canonical glutamine metabolism for therapeutic intervention. This may provide novel in-sights and opportunities for exploiting glutamine metabolic reprogramming in PC treatment.
Macranthoside B Suppresses the Growth of Adenocarcinoma of Esophagogastric Junction by Regulating Iron Homeostasis and Ferroptosis through NRF2 Inhibition
Authors: Wang Lingling , Pan Guangzhao , Tian Sichao , Zhang Che , Tao Fangfang , Qin Jiang-Jiang
Published: January 2025

Background:

Macranthoside B (MB) is a saponin compound extracted from hon-eysuckle that has been reported to exhibit significant medicinal values, particularly anti-tumor activities. This study aimed to evaluate the anticancer efficacy of MB in treating adenocarci-noma of the esophagogastric junction (AEG) and elucidate its underlying mechanisms.

Methods:

Three AEG cell lines and normal gastric epithelial cells were used to assess the an-ticancer activity of MB in vitro. A series of experiments, including RNA sequencing (RNA-seq) analysis, transmission electron microscopy (TEM), immunofluorescence, and western blot assay, were conducted to validate the molecular mechanisms by which MB may mediate these physiological changes. Finally, we used shRNA assays to silence the key gene driving these changes and examined the expression of molecules involved in the affected pathways.

Results:

MB exhibited significant anti-AEG cell activity with IC50 values ranging from 9.5 to 12.7 μM. RNA-seq results indicated that MB treatment in AEG cells significantly altered mRNA levels of autophagy- and ferroptosis-related genes. Further experiments revealed that MB treatment led to the up-regulation of lipid reactive oxygen species (Lip-ROS), oxidative stress-related pathway genes, and LC3B-labeled autophagic vesicles in AEG cells. Moreover, MB mediated NCOA4-dependent ferritinophagy, disrupting iron homeostasis and causing subsequent ferroptosis. We further confirmed that the intrinsic connection between autophagy and ferroptosis was due to the inhibition of NRF2 by MB. The inhibition of NRF2 by MB triggered transcriptional repression of its downstream effector molecules HERC2 and VAMP8, thus stabilizing NCOA4.

Conclusion:

This study demonstrated MB to inhibit AEG cell growth by regulating iron ho-meostasis and inducing ferroptosis through the inhibition of NRF2, providing a basis for the development of novel drugs for AEG treatment
SRT1720 Treatments Hepatic Ischemia Reperfusion Injury by Regulation of NF-κB Signaling Pathways and Reduce Cell Apoptosis: From Network
Pharmacology to Experimental Validation
Authors: Li Zhongzhe , Geng Wenting , Yu Beilei , Wang Bin , Sun Shuxuan , Zhou Lu
Published: January 2025

Background and objective:

Hepatic ischemia reperfusion injury (HIRI) is a common
complication closely related to the prognosis of liver surgery, and effective treatment methods
are still unavailable. SRT1720 has the characteristics of multifunction and multitarget which may
cope with the multidirectional complex pathological process caused by HIRI. The present study
aimed to explore the potential mechanism of SRT1720 in HIRI through a combination of network
pharmacology, in vitro experiments and in vivo models.

Methods:

Differentially expressed genes (DEGs) were identified based on the GSE15480 and
Genecards database. Enrichment analyses were then conducted. SRT1720-targeted genes were
obtained through databases such as Chembl, TTD, GtoPdb, and so on. All target genes were
standardized by the Uniprot database and the Kyoto Encyclopedia of Genes and Genomes
(KEGG) pathways were identified by STRING. Shared KEGG pathways were identified using a
Venn diagram among SRT1720-targeted pathways and HIRI. Furthermore, experimental techniques
such as cell apoptosis assay and western blotting were used to confirm the most significant
biological processes and the key pathway between SRT1720-targeted and HIRI.

Results:

This study identified 118 HIRI-related DEGs, 69 shared KEGG pathways of SRT1720
and HIRI. In addition, the findings revealed that SRT1720 significantly reduced liver ischemiareperfusion
(I/R) injury. NF-κB signaling pathway and the expression of promoting apoptosis
factors such as Bax and Caspase3 were inhibited, while antiapoptotic protein Bcl-2 was promoted
in the SRT1720 group compared with the I/R group.

Conclusion:

The findings indicate that SRT1720 may inhibit the development of HIRI by inhibiting
the NF-κB signaling pathway and reducing cell apoptosis, acting as a treatment for HIRI.
First Report of HPV Genotyping and Distribution in People Living with
and Without HIV from Iran and the Middle East
Authors: Maleki Faezeh , Farahmand Mohammad , Keyvani Hossein
Published: January 2025

Aims:

In people living with human immune deficiency (PLHIV), the rates of human papillomavirus
(HPV) infection, mixed types, and high-risk (HR) strains increase, while the virus
clearance is prevented. Here, we report HPV genotyping in PLHIVs from Iran and the Middle
East region for the first time.

background:

In people living with human immune deficiency (PLHIV), the rates of human papillomavirus (HPV) infection, mixed types, and high-risk (HR) strains increase, while the virus clearance would be called off.

Methods:

HPV genotyping in referring individuals from different provinces to our laboratory was
evaluated over 2023-2024. For this, the HPV types in specimens were detected through the INNO-
LiPA HPV genotyping kit. Statistical analysis was conducted with a 95% confidence interval
(95%CI) and P < 0.05. Accordingly, 481 subjects from various provinces participated in this
study.

Results:

The rate of HPV infection was 45.7%, of which 14% were HIV-infected women referred
from all provinces. The most prevalent types included 6, 51, and 18, but not 16 HR types. Mixed
infections in dually infected women were significantly more than in HPV-infected ones. The
HPV+/HIV+ subgroup had the lowest median age. The prevalence of HPV types and mixed infection
in PLHIVs was congruent with the previous reports, except for the low rate of type 16 infection,
perhaps due to the healthy nature of our subjects. Only HIV+/HPV+ cases' age was similar
to the previous reports, perhaps because of sample collection and study designs. Among all factors,
age and gender affected the HPV type distribution notably.

Conclusion:

The current study corroborated the results of many prior reports, demonstrating the
considerable impact of HIV status on HPV distribution. The authors recommend implementing a
national HPV vaccination and more comprehensive reports of HPV genotyping in PLHIVs.

other:

NA
Peptic Ulcer Disease: A Comprehensive Review on Conventional Therapy and Herbal Treatment
Authors: Kumar Vijay , Goel Vrinda , Bajaj Sakshi , Garg Kalpana , Bhargava Anurag , Dhingra Ashwani K.
Published: January 2025

Still today, peptic ulcer disease (PUD) is a major digestive illness that affects millions of
people around the world every year. This study looks at both traditional and herbal ways of treating
PUD, focusing on how they work, how well they work, and whether they can work together. Pharmaceuticals
like antibiotics, proton pump inhibitors (PPIs), and H2-receptor antagonists are common
ways to treat the condition. In more serious cases, surgery may also be an option. The use of medical
plants in phytotherapy, an herbal treatment, has shown promise in helping people with PUD deal with
their symptoms and speed up the healing of ulcers. Citrus maxima (Pomelo), a plant in the Rutaceae
family, may be able to help treat PUD because it has functional and antioxidant properties. In addition,
studies on how well different plants treat PUD have shown that they have anti-inflammatory
and cytoprotective qualities. Also, the parts of photosynthesis that plants use contain many beneficial
substances that might help with the treatment of PUD. This all-around method would deal with many
pathogenic pathways at the same time. More study needs to be done to learn more about how herbal
therapies can be used to treat PUD and how they can be combined with other common treatments.
Efficacy of Ozonated Olive Oil as a Topical Treatment for Dermatophytosis Caused by T. mentagrophytes in Rat Model
Authors: Mohaghegh Ali , Mansouri Mahdi , Etemad Leila , Soheili Vahid , Haghighzadeh Atoosa , Dadpour Saba , Rajabi Omid
Published: January 2025

Introduction:

Dermatophytosis is a significant global health issue that poses a serious risk
to public well-being. This fungal infection occurs when dermatophytes invade the outer surfaces of
both human and animal bodies. The use of common anti-dermatophyte medications has some limitations
due to their side effects and drug resistance. Herbal remedies offer broad-spectrum antimicrobial
effects low toxicity, and are widely used compared to traditional chemical fungicides. Recent studies
have indicated that the ozone has antifungal activity due to its high oxidizing power and skin
penetrating capacity.

Methods:

This study examined the antifungal effects of ozonated olive oil in treating dermatophytosis
caused by Trichophyton mentagrophytes through in vitro and in vivo tests on rats. The agar dilution
method was used to establish the treatments' minimum inhibitory concentrations (MICs). For the
in vivo study, thirty male Wistar albino rats from the Laboratory Animal Unit were prepared. The
topical antifungals were then applied topically once daily for 7 days to the animals beginning 10 days
after the appearance of symptoms. Five groups of animals were treated with virgin olive oil, ozonated
olive oil (1.25, 2.5, or 5 mg/mL), and terbinafine (1.25 g/mL).

Results:

Following the therapy period, clinical and mycological efficacies were assessed. Ozonated
olive oil and terbinafine were shown to have MIC of 2.5 μg/mL and 0.62 μg/mL against T. mentagrophytes,
respectively. Ozonated olive oil showed antifungal effects and significantly reduced the
clinical score by increasing hair growth and decreasing inflammation, similar to terbinafine.

Conclusion:

Therefore, ozonated olive oil may serve as an alternative treatment for Trichophyton
infections.
Phillygenin: A Versatile Multi-Targeted Anti-inflammatory, Hepatoprotactive
and Anticancer Agent
Authors: Tailor Navin Kumar
Published: January 2025

Background:

Multi-target drugs are gaining attention for treating chronic diseases. Phillygenin (C21H24O6), a compound in Forsythiae Fructus, demonstrates strong anti-inflammatory, antifibrotic, anticancer, antioxidant, and antimicrobial properties. Its polar functional groups allow for
structural modifications, offering the potential for novel drug discovery and development.

Objective:

This review provides an overview of Phillygenin as a scaffold for multi-target drug development against chronic disease and disorders. It examines the molecular mechanisms behind its therapeutic effects, focusing on targets like NF-κB, PI3K/AKT, MAPK, Nrf2-ARE, P2X7R/NLRP3,
Ca2+-calcineurin-TFEB, JAK/STAT, Notch1, TGF-β/Smads, and AMPK/ERK/NF-κB.

Methods:

A systematic review of the literature on Phillygenin was conducted to explore its therapeutic applications. The study examined Phillygenin's molecular mechanisms and potential targets to
highlight its multi-targeting capabilities.

Results:

Phillygenin shows promise in managing chronic diseases by targeting multiple cellular
pathways, including NF-κB, PI3K/AKT, MAPK, Nrf2-ARE, P2X7R/NLRP3, Ca2+-calcineurinTFEB, JAK/STAT, Notch1, TGF-β/Smads, and AMPK/ERK/NF-κB. Its versatility and natural
origin make it a valuable scaffold for developing multi-target therapeutics.

Conclusion:

Exploring Phillygenin as a framework for multi-target drugs offers the potential to
boost efficacy and reduce side effects. Further research and clinical trials are needed to confirm Phillygenin's therapeutic potential.
Aberrant Expressions of EDNRB and EDN3 in a Multifactorial Hirschsprung Disease
Authors: Gunadi . , Vujira Khanza Adzkia , Amadeus Verrell Christopher , Gabriela Gita Christy , Amaragati Adisrasti Rejeki , Geometri Esensi Tarian , Eryvinka Laudria Stella , Ferdinandus Kevin Eliezer , Pramono Nabila Ardia , Dwihantoro Andi
Published: January 2025

Background:

Hirschsprung disease (HSCR) is a multifactorial disorder due to the enteric
nervous system (ENS) development failure. At least 35 genes have been responsible for HSCR,
including EDNRB and EDN3. Here, we aimed to determine the EDRNB and EDN3 expressions effects
in HSCR subjects.

Methods:

Our study analyzed EDNRB and EDN3 expressions in the colon of HSCR subjects and
controls by a quantitative PCR. The EDNRB and EDN3 expressions were analyzed by the Livak
method (2-ΔΔC
T).

Results:

Twenty-seven HSCR patients and 20 controls were ascertained. EDNRB and EDN3 expressions
downregulated was found in ganglionic and aganglionic HSCR than control colons (EDNRB:
ΔCT 6.78 ± 1.38 vs. 1.71 ± 2.79; p = 0.0001 (ganglionic); ΔCT 4.41 ± 1.63 vs. 1.71 ± 2.79; p
= 0.0005 (aganglionic); and EDN3: ΔCT 7.60 ± 1.93 vs. 1.81 ± 2.89; p = 0.0001 (ganglionic); ΔCT
9.72 ± 4.32 vs. 1.81 ± 2.89; p = 0.0001 (aganglionic)). A significant difference in EDNRB and
EDN3 expressions was also noted between the HSCR colon: ganglionic vs. aganglionic segment
(p = 0.00002 and 0.017).

Conclusion:

We report the downregulated EDNRB and EDN3 expressions in HSCR subjects, indicating
EDNRB/EDN3 expressions have a significant responsibility in HSCR pathogenesis. Nevertheless,
we further clarify the complexity of the development of ENS.
Modulation of Bone Turnover Markers with Dipeptidyl Deptidase-4:
Interplay in Bone Health
Authors: Ahmed Faraha , Ahmad Syed Sufian , Najmi Abul Kalam , Khan Mohammad Ahmed
Published: January 2025

Understanding the structure, development, and maintenance of bone is crucial to the assessment of bone health and interpretation of clinical outcomes. Bone turnover markers released
during bone remodeling have been extensively investigated in osteoporosis studies. These markers
include bone formation markers, which are involved in osteogenesis, and bone resorption markers,
which break down the bone structure. Although these markers complement bone mineral density
results, their clinical use faces challenges because of fluctuations related to factors such as age,
gender, and circadian rhythms, as well as analytical variability. Dipeptidyl peptidase-4 (DPP-4) is
a ubiquitous exopeptidase that regulates the functions of various proteins. It is present in bound
and free form and circulates freely in the extracellular space. It regulates glucose metabolism, cell
migration and differentiation, oxidative stress, inflammation, cellular signaling, and the immune
system. It also plays an important role in collagen metabolism due to its presence in osteocytes, osteoclasts, and osteoblasts. The substrates of DPP-4, like gastrointestinal peptides, incretins, and
neuropeptides, influence bone homeostasis as well via modulation of bone markers. This review
explores bone turnover markers in relation to DPP-4 to better understand their role in bone
metabolism concerning osteoporosis.
Optimized Rutin-incorporating PEGylated Nanoliposomes as a Model wit Remarkable Selectivity Against PANK1 and MCF7 Cell Lines
Authors: Al-Samydai Ali , Al Qaraleh Moath , Al-Halaseh Lidia K. , Abu Hajleh Maha N , Carradori Simone , Abdulmaged Maryam , Kareem Rand , Alzaidi Hasanain , AK Mousa Mohamad , Al-Hiari Yusuf , Nsairat Hamdi , Alshaer Walhan
Published: January 2025

Background:

This study aims to enhance the delivery of polyphenols using nanotechnology.

Objective:

To develop and evaluate liposomal formulations for improved delivery and stability of polyphenols,
specifically focusing on Rutin.

Methods:

Liposomal formulations were meticulously prepared via the Thin-Film Hydration method. Comprehensive
physical characterization was conducted, including stability assessments using Dynamic Light Scattering
(DLS) and Thermogravimetric Analysis (TGA). The free radical scavenging activity was measured using the
DPPH• assay, and MTT cell viability assays were performed to assess cytotoxicity.

Results:

The results demonstrated a significant reduction in nanoparticle size from 123 nm to 116 nm and an
increase in charge from -14 to -22 with rising Rutin concentrations. The formulation achieved enhanced homogeneity
at a Rutin concentration of 2.0 mg/mL and showed higher stability. Incorporating Rutin improved the formulation's
stability over 30 days, as evidenced by a decrease in the Differential Scanning Calorimetry peak temperature
from 58.65 °C to 54.42 °C. Rutin-loaded and co-loaded nanoliposomes exhibited remarkable selectivity
against PANK1 and MCF7 cell lines, with IC50 values of 2.13±0.35 μg/mL and 4.75±0.19 μg/mL, respectively.

Conclusion:

PEGylated Rutin-loaded nanoliposomes offer a promising platform for biodegradable and biocompatible
drug delivery systems, enhancing the bioavailability, solubility, and stability of the polyphenols.
Treatment of Primary Hypothyroidism by Slow-Release Liothyronine Monotherapy
Authors: Azizi Fereidoun , Amouzegar Atieh , Abdi Hengameh , Masoumi Safdar , Mehran Ladan
Published: January 2025

Background:

Combination therapy with levothyroxine (L-T4) and slow-release T3 (SRT3)
in the treatment of hypothyroidism results in a normal triiodothyronine/thyroxine (T3/T4) ratio
above that of L-T4 monotherapy. No clinical study has been reported on SRT3 monotherapy
for hypothyroidism.

Methods:

This study was conducted in two parts. In the first part, 20 patients with primary hypothyroidism
and serum thyrotropin (TSH) >30 mU/L were randomized into three groups receiving
1.6 μg/kg L-T4, equivalent doses of SRT3 or L-T3 of 0.55 μg/kg for 4 weeks. Their fasting
serum-free T4 [fT4], T3, and TSH were measured weekly before taking medication for up to 4
weeks. In the second part, in 9 hypothyroid patients on L-T4 therapy and normal serum TSH, LT4
therapy was discontinued, and a once-daily dose of SRT3 of 0.55 μg/kg was replaced. Serum
fT4, T3, and TSH were measured weekly.

Results:

In part one, in patients treated with L-T3 and L-T4, serum TSH decreased to normal values
after 4 weeks of intervention. In 7 patients on SRT3, serum T3 increased from 47±12 at baseline
to 110±16 ng/dL, and serum TSH decreased from 60±11 at baseline to 24±10 and 26±7
mU/L, respectively, at 14 and 21 days after intervention. At the end of 28 days, mean serum T3
was 110±16, 168±74, and 96±18 ng/dL in SRT3, L-T3, and L-T4 groups, respectively (p <
0.001). In part two, serum fT4 decreased from 1.43±0.7 to 0.41±0.14 ng/dl, and serum T3 increased
from 86±21 to 113±27 ng/dL by 21 days. Mean serum TSH remained normal until 14
days but increased to 15.1±7.6 mU/L at 21 days. In the end, mean serum fT4, T3, and TSH were
0.35±0.17 ng/dl, 77.4±8.9 ng/dL, and 35±11 mU/L, respectively.

Conclusion:

In patients with primary hypothyroidism, SRT3 monotherapy with an equivalent
dose to L-T4 maintained normal serum T3 but could not sustain normal serum TSH concentration.
The Role of the Toll-Like Receptor Signaling Pathway in Autoimmune Diseases and Treatment with Traditional Chinese Medicine: A Literature Review
Authors: Wang Yaru , Bao Lin , Liu Mingying , Tang Xintian , Liu Hui , Liang Xiaodong , Liu Yifan
Published: January 2025

Toll-Like Receptors (TLRs) is a pattern recognition receptor that connects innate and
adaptive immunity and participates in inflammatory responses play a key role in common autoimmune
diseases such as Rheumatoid Arthritis (RA), systemic lupus erythematosus (SLE), psoriasis,
and Sjögren’s syndrome (SS) by participating in antigen recognition, immune cell activation, and
inflammatory factor release. Due to the multi-component and multi-target characteristics of traditional
Chinese medicine (TCM), the role of TCM active ingredients acting on TLRs has been
widely studied. This article describes the relationship between TLR and four autoimmune diseases,
as well as a review of the efficacy of TLR intervention by active ingredients of traditional Chinese
medicine. To provide some basis for the future clarification of the mechanism of action of
drugs for autoimmune diseases and to assist in the development of new medicines.
Prolonged Sitting and Low Back Pain in Dental Practice: A Complex Relationship Explored in Jakarta
Authors: Hendarmin Laifa Annisa , Zaki Achmad , Andini Dela , Mardjikoen Bisatyo , Rufaidah Melia Fatrani , Thaiyibah Nazaratun
Published: January 2025

Aims & Background

Low back pain (LBP) is the leading cause of disability globally, affecting approximately 619 million people in 2020, with projections suggesting that this number will rise to 843 million by 2050. LBP accounts for a significant proportion of years lived with disability (YLDs) worldwide. Dentists are particularly vulnerable to LBP due to the prolonged static and non-ergonomic postures required to access small areas within the oral cavity, which often results in extended examination times. Poor posture, such as prolonged standing or sitting, is a recognized risk factor for developing LBP. This study aims to examine the relationship between prolonged sitting duration during dental practice and the prevalence of LBP among dentists in Jakarta.

Materials and Methods

A cross-sectional study was performed involving 106 dentists, chosen through quota sampling based on specific inclusion and exclusion criteria. Data were gathered using a standardized questionnaire and the Roland-Morris Disability Questionnaire (RMQ) to assess functional impairment due to LBP. The Chi-Square test was used to analyse the association between sitting duration and LBP complaints.

Results

The results showed no significant association between prolonged sitting duration (p = 0.617) or physical activity (p = 0.662) and the prevalence of LBP. However, age was significantly associated with LBP prevalence (p = 0.008), with dentists aged 46–65 years reporting a higher prevalence. Additionally, years of practice approached statistical significance (p = 0.057), suggesting a potential link between prolonged occupational exposure and the risk of LBP. Despite the lack of significance for sitting duration and physical activity, the findings highlight the importance of considering other factors, such as chair design, posture, movement patterns, and the cumulative impact of years of practice, that may contribute to LBP in this professional group.

Conclusion

Age and years of practice are important factors contributing to the prevalence of LBP among dentists, emphasizing the impact of age-related changes and prolonged occupational strain. While sitting duration and physical activity showed no significant association, the findings underscore the need for targeted ergonomic interventions and preventative strategies tailored to the specific demands of dental practice.
Adherence to Radiation Protection Principles in the Clinical Environment among Anesthesia and Operating Room Nursing Students: Implications for Educational Interventions
Authors: Ghasemi Sina , Khosravi Hossein , Imani Behzad , Shabani Naeeme
Published: January 2025

Background

The increasing use of ionizing radiation in clinical environments, particularly during surgeries such as orthopedics and neurosurgery, has heightened concerns about the potential health risks associated with this type of radiation. This issue is particularly relevant for nursing students undergoing training in operating room settings, where they are frequently exposed to various sources of radiation. To effectively implement radiation protection principles, it is crucial to evaluate the knowledge and attitudes of the students toward ionizing radiation. Understanding their level of awareness and beliefs about radiation safety will inform the development of targeted training programs aimed at minimizing exposure and enhancing safety in clinical environments.

Objective

This study aimed to investigate the level of knowledge, attitudes, and performance of 8th-semester undergraduate anesthesia and operating room nursing students toward radiation protection principles.

Methods

This cross-sectional study was conducted at the Hamadan University of Medical Sciences in 2024. In this study, students were selected via the census sampling method. The data related to the study were collected from valid questionnaires concerning the participants’ level of knowledge, attitudes, and performance toward ionizing radiation. The data analysis was also conducted at the descriptive and inferential statistics level via SPSS version 16 software.

Results

The knowledge, attitudes, and performance of the students in both fields were inadequate compared to the ideal standards. Additionally, the mean attitude score toward protective principles was significantly greater among operating room nursing students than anesthesia nursing students (p-value = 0.023). Furthermore, a positive and significant correlation was found between students' Grade Point Average (GPA) and their scores on knowledge and attitudes toward protective principles (r = 0.449, p-value = 0.000 and r = 0.648, p-value = 0.000, respectively).

Conclusion

This study revealed that the level of knowledge among students regarding ionizing radiation is lower than the desired standards for ensuring safety in clinical environments. As a result, it is essential to implement targeted training courses to enhance students' understanding of the risks associated with ionizing radiation.
Synthesis and Characterization of Thioamidoalkyl Fluorescein Analogs for Antibacterial Activity and Molecular Docking Analyses
Authors: Kousar Shazia , Tabassum Maryam , Qaid Saif Mabkhot , Mohamed Yusof Nur Intan Saidaah , Mohd Fauzi Fazlin , Ali Shah Syed Adnan , Shahid Muhammad , Irfan Madiha , Monawar Ayesha , Bukhari Syed Waqas , Mudassir Muhammad Ahmad
Published: January 2025

Aims and Objectives:

The study aimed to employ one-pot solvent-free boric acid-catalyzed
multicomponent reactions (MCRs) to synthesize bioactive thioamidoalkyl fluorescein analogs.
The study aimed to introduce a facile and environmentally sustainable strategy for efficiently
producing alternate potent bioactivity agents.

Background:

Population growth trends, limited efficacy and side effects of available medicine, and
new challenges like antibiotic resistance have led to the urgent need for more and better pharmaceuticals
and a notable increase in drug development. The global health demands and significant
medicinal value of thioamidoalkyl compounds prompted synthesizing new fluorescein-based thioamidoalkyl
derivatives to explore their prospective biomedical potential.

Methods:

To prepare thioamidoalkyl fluorescein analogs, a solvent-free three-component reaction
of fluorescein with aryl aldehydes and thiobenzamide catalyzed by boric acid was used. The antibacterial
potentials of thioamidoalkyl fluorescein analogs against Escherichia coli (E. coli) bacteria
were analyzed in terms of half-maximal inhibitory concentration (IC50). Moreover, molecular docking
experiments explored the binding affinities and possible interaction mechanisms between newly
synthesized analogs and active sites of E. coli adhesion protein FimH.

Results:

FTIR, 1H, and 13C NMR results verified the successful formation of all analogs. The
experimental and theoretical antibacterial activity results confirmed that the compound M-11 is
relatively more potent against E. coli based on lower IC50 values of 54.14 nM and binding energy
value of ‒6.30 kcal/mol (comparable to ‒6.70 kcal/mol of reference ligand) probably because of
unique structure and strong binding affinities for target protein structure.

Conclusion:

The findings demonstrated the potential of the currently employed synthetic approach
to produce new analogs with decent yields facilely. Interestingly, the M-11 compound proved to be
an excellent prospective source of antibiotic drugs based on both experimental and computational
analyses.
Impact of Green Synthesis of Ag-nanoparticle Using Extracellular
Manganese Peroxidase on Different Dye Degradation
Authors: Hoque Rohida Amin , Yadav Meera
Published: January 2025

Introduction:

The study explores the biosynthesis of silver nanoparticles (AgNPs) using
extracellular manganese peroxidase enzyme from Trichoderma parestonica. The synthesis was optimized
at a 1:1 enzyme and silver nitrate ratio, pH 12, shaking process, and 48-hour synthesis
period. The AgNPs were characterized using spectroscopic and microscopic techniques, showing
absorbance in UV-spectroscopy between 410-450 nm due to Surface Plasmon Resonance (SPR).

Method:

The stabilization of extracellular manganese peroxidase with the nanoparticles through
capping was observed by Fourier Transform Infrared Spectroscopy (FT-IR). The spherical shape
of the AgNPs, with an average size of 69.09 nm, is confirmed by the Field Emission Scanning
Electron Microscopy (FESEM) study. The size of the nanoparticles was also determined by Dynamic
Light Scattering (DLS) to be 75.99 nm. When synthesized AgNPs were used to decolorize
Alizarin red S (ARS), Methylene Blue (MB), and Methyl Orange (MO) in the presence of sodium
borohydride reducing agent, the results showed that, within 20 minutes, 90% of 0.1mM ARS, MB,
and 75% 0.1mM MO were degraded.

Result:

This study demonstrated the potential of AgNPs synthesized from MnP enzyme in nanoremediation
projects, offering a sustainable solution to the problems and issues of dye-induced
wastewater pollution and fostering environmental conservation.

Conclusion:

Enzymes are being studied in nanotechnology, leading to the development of enzyme
nanoparticles, which can be utilized in various fields like biosensors agriculture, drug delivery, and
bioremediation.

other:

Key-words: Manganese peroxidase; Silver nanoparticles; Dye decolorization; Alizarin red S, Methylene blue, Methyl orange