bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Activity, Body Mass, and Adherence to the Mediterranean Diet in Preschool Children: A Cross-Sectional Analysis in the Split-Dalmatia County (Croatia)
Authors: Obradovic Salcin Lejla , Karin Zeljka , Miljanovic Damjanovic Vesna , Ostojic Marko , Vrdoljak Andrea , Gilic Barbara , Sekulic Damir , Lang-Morovic Maja , Markic Josko , Sajber Dorica
Published: September 2019

Physical activity, body mass, and dietary habits are known to be important determinants of overall health status, but there is an evident lack of studies that examine these issues specifically in preschool children. The aim of this study was to identify associations that may exist between adhering to the Mediterranean diet (MD), levels of physical activity (PA), and body composition indices in apparently healthy preschool children from southern Croatia. Participants were 5- to 6-year-old preschoolers from the Mediterranean part of the country (the Split-Dalmatia County; n = 260, 126 females). Adherence to the MD was observed by the Mediterranean Diet Quality Index (KIDMED), PA level was evaluated by the Preschool-age Children’s Physical Activity Questionnaire (Pre-PAQ), and responses were collected from the parents. The participants’ waist circumferences (in cm), waist-to-hip ratios, and body mass index (in kg/m2, and in a z-score calculated relative to the normative value for age and sex) were used as indicators of body composition. All children were of the same age and tested over a one-month period of the same year as a part of the regular examination undertaken before attending elementary school. With only 6% of the children having a low KIDMED score, adherence to the MD was high. MD adherence was higher in girls (Chi-square = 15.31, p < 0.01) and children who live on the coast of the Adriatic Sea (Chi-square = 18.51, p < 0.01). A mixed effects logistic regression (with kindergarten as random factor) identified sedentary activity to be negatively associated with MD adherence (OR per point: 0.65, 95% CI: 0.44–0.91). High adherence to the MD in the studied sample may be attributed to regulated feeding in kindergarten. Considering that most Croatian elementary schools do not provide food to their students, MD adherence should be investigated later in life and also in other parts of the country where the MD is culturally less prevalent.
The “Motor of the Day”: Parent and School-Age Children’s Cognitions, Barriers, and Supports for Breakfast
Authors: Eck Kaitlyn M. , Delaney Colleen L. , Clark Rashel L. , Leary Miriam P. , Shelnutt Karla Pagan , Olfert Melissa D. , Byrd-Bredbenner Carol
Published: September 2019

Despite the many benefits of regular breakfast consumption few parents and children consume this meal every day and research examining the determinants of breakfast consumption is limited. Thus, the purpose of this study was to examine breakfast-related cognitions (i.e., beliefs, attitudes, facilitators, barriers) of parents and school-age children (ages 6–11 years) using the constructs of Social Cognitive Theory as a guide. Parents (n = 37) and children (n = 41) participated in focus group discussions held in community settings in 3 states (FL, NJ, WV). Data were qualitatively content analyzed to detect trends. Parents felt breakfast was important for numerous reasons. Parents expressed concern about the healthfulness of some traditional breakfast options, preferring breakfasts containing mostly fruits, vegetables, and protein and fewer carbohydrates. Parents identified lack of time as the greatest barrier to breakfast consumption. To overcome this barrier, they utilized grab-and-go foods, prepared breakfast ahead of time, and got up earlier. Utilizing the school breakfast program was another strategy mentioned, however some were concerned about the nutritional quality of this option. Children recognized the importance of breakfast and cited several benefits. The greatest barrier to breakfast identified by children was feeling rushed in the morning. To overcome barriers, children suggested having a morning routine, selecting or preparing breakfast foods ahead, and relying on parents to encourage breakfast consumption. The effectiveness of interventions aiming to improve breakfast intake may be improved by addressing parent and child breakfast-related cognitions and tailoring interventions to address their needs.
A Multicentre Study of Psychological Variables and the Prevalence of Burnout among Primary Health Care Nurses
Authors: Ortega-Campos Elena , Cañadas-De la Fuente Guillermo A. , Albendín-García Luis , Gómez-Urquiza José L. , Monsalve-Reyes Carolina , de la Fuente-Solana E. Inmaculada
Published: September 2019

Nurses in primary health care (PHC) have multiple responsibilities but must often work with limited resources. The study’s aim was to estimate burnout levels among PHC nurses. A Quantitative, observational, cross-sectional, multicentre study of 338 nurses working in PHC in the Andalusian Public Health Service (Spain) is presented. A total of 40.24% of the nurses studied had high levels of burnout. The dimensions of emotional exhaustion and depersonalisation were significantly associated with anxiety, depression, neuroticism, on-call duty and seniority-profession and inversely related to agreeableness. In addition, depersonalisation was significantly associated with gender, and emotional exhaustion correlated inversely with age. Personal achievement was inversely associated with anxiety and depression and positively correlated with agreeableness, extraversion and responsibility. There is a high prevalence of burnout among nurses in PHC. Those most likely to suffer burnout syndrome are relatively young, suffer from anxiety and depression and present high scores for neuroticism and low ones for agreeableness, responsibility and extraversion.
Importance of Agility Performance in Professional Futsal Players; Reliability and Applicability of Newly Developed Testing Protocols
Authors: Sekulic Damir , Foretic Nikola , Gilic Barbara , Esco Michael R. , Hammami Raouf , Uljevic Ognjen , Versic Sime , Spasic Miodrag
Published: September 2019

The purpose of this study was to determine the inter- and intra-testing reliability of newly developed tests of the change of direction speed (CODS) and reactive agility (RAG) in competitive futsal players. Additionally, the developed tests were evaluated for their validity with regard to the differentiation of two performance-levels. Thirty-two professional male futsal players (age = 26.22 ± 5.22 years; body height = 182.13 ± 5.99 cm, body mass = 77.43 ± 8.00 kg) participated in the study. The sample was divided into two groups based on their level of futsal performance: A top-level-group (n = 12) and a team-level-group (n = 20). The variables included body height, mass, body mass index, a sprint over a 10-m distance (S10M), and eight newly developed futsal specific CODS and RAG tests. The CODS and RAG tests were performed by dribbling the balls (CODS_D and RAG_D) and without dribbling (CODS_T and RAG_T), and the performances on the dominant and non-dominant sides were observed separately. All CODS, and RAG tests performed on dominant side and RAG_T tests performed on the non-dominant side had good inter-testing (CV = 5–8%; ICC = 0.76–0.89) and intra-testing (CV = 4–9%; ICC = 0.77–0.91) reliability. However, RAG_D performed on the non-dominant side was not reliable (ICC = 0.60, CV = 10%). The top-level-players outperformed the team-level-players in the CODS and RAG tests that involved dribbling (t-test: 4.28 and 2.40, p < 0.05; effect sizes (ES): 0.81 and 1.5, respectively), while the team-level players achieved better results in the CODS_T (t-test: 2.08, p < 0.05; ES: 0.60). The proposed CODS and RAG tests that involved dribbling over a 3.2-m distance, especially on the dominant side, appeared to be reliable, as well as valid for distinguishing the performance level in futsal players.
Do Health-Related Quality of Life and Pain-Coping Strategies Explain the Relationship between Older Women Participants in a Pilates-Aerobic Program and Bodily Pain? A Multiple Mediation Model
Authors: Ruiz-Montero Pedro Jesús , Ruiz-Rico Ruiz Gerardo José , Martín-Moya Ricardo , González-Matarín Pedro José
Published: September 2019

This study (1) analyzes the differences between non-participating and participating older women in terms of clinical characteristics, pain coping strategies, health-related quality of life and physical activity (PA); (2) studies the associations between non-participants and participants, clinical characteristics, pain coping strategies, HRQoL and bodily pain and PA; and (3) determines whether catastrophizing, physical role, behavioural coping, social functioning and emotional role are significant mediators in the link between participating in a Pilates-aerobic program (or not) and bodily pain. The sample comprised 340 older women over 60 years old. Participants of the present cross-sectional study completed measures of clinical characteristics: HRQoL using the SF-36 Health Survey, pain-coping strategies using the Vanderbilt Pain Management Inventory (VPMI) and PA using the International Physical Activity Questionnaire (IPAQ). Significant differences between non-participants and participants, were found in clinical characteristics, pain-coping strategies (both, p < 0.05), HRQoL (p < 0.01), and PA (p < 0.001). Moreover, catastrophizing support mediated the link between non-participants and participants and bodily pain by 95.9% of the total effect; 42.9% was mediated by PA and 39.6% was mediated by behavioural coping. These results contribute to a better understanding of the link between PA and bodily pain.
The Association of Internet Use with Wellbeing, Mental Health and Health Behaviours of Persons with Disabilities
Authors: Duplaga Mariusz , Szulc Katarzyna
Published: September 2019

Introduction: There is strong evidence that people with disabilities suffer from a significant digital divide. However, there are reports indicating that Internet use may result in many benefits for those with disabilities. The aim of the study was to assess the impact that the use of the Internet has on the wellbeing and health behaviours of persons with disabilities. Methods: An analysis was carried out using the dataset obtained from Social Diagnosis, a panel study undertaken on a nationally representative sample. The records of persons with disabilities were retrieved from the dataset which was established in 2015. An analysis of the association between Internet use and the wellbeing, mental health and health behaviours of the respondents was undertaken. The variables reflecting the self-assessment of their own life and experience of loneliness were treated as being indicators of their wellbeing and the prevalence of suicidal thoughts or making use of psychological help as indicators of mental health. The health behaviours analysed in the study included smoking, excessive consumption of alcohol and undertaking physical activity. For all these variables, multivariate logistic regression models were developed. The effect of Internet use was adjusted for sociodemographic variables and the degree of disability. An analysis was performed after applying post-stratification weights available from the Social Diagnosis study. Results: The weighted study group consisted of 2529 people having a mean age of 59.33 ± 16.89 years. The group included 20.71% (N = 524) respondents with a mild, 41.58% (N = 1052) with a moderate, and 26.54% (N = 671) with a severe disability. The proportion of Internet users was 37.07% (N = 937). In all the regression models, Internet use had a significant impact on the dependent variables. After adjustment for sociodemographic variables and the degree of disability, the Internet users more frequently assessed their lives as happy (odds ratio, 95% confidence interval: 1.86, 1.47–2.37) and less frequently experienced loneliness (0.63, 0.49–0.81) or suicidal thoughts (0.47, 0.35–0.65). In addition, they needed psychological help less frequently (0.50, 0.35–0.72). Interestingly, Internet users undertook some form of physical activity or sport more often (2.41, 1.87–3.13) and fewer smoked cigarettes (0.70, 0.54–0.91) or consumed alcohol excessively (0.32, 0.19–0.56). Conclusions: The use of the Internet by people with disabilities was associated with improved wellbeing, better mental health and more beneficial health behaviours. These findings support the development of intensive actions to reduce the digital divide for the population of people with disabilities.
Assessing Risk of E. coli Resuspension from Intertidal Estuarine Sediments: Implications for Water Quality
Authors: Wyness Adam J. , Paterson David M. , Rimmer James E. V. , Defew Emma C. , Stutter Marc I. , Avery Lisa M.
Published: September 2019

Estuarine sediments are a reservoir for faecal bacteria, such as E. coli, where they reside at greater concentrations and for longer periods than in the overlying water. Faecal bacteria in sediments do not usually pose significant risk to human health until resuspended into the water column, where transmission routes to humans are facilitated. The erosion resistance and corresponding E. coli loading of intertidal estuarine sediments was monitored in two Scottish estuaries to identify sediments that posed a risk of resuspending large amounts of E. coli. In addition, models were constructed in an attempt to identify sediment characteristics leading to higher erosion resistance. Sediments that exhibited low erosion resistance and a high E. coli loading occurred in the upper- and mid-reaches of the estuaries where sediments had higher organic content and smaller particle sizes, and arose predominantly during winter and autumn, with some incidences during summer. Models using sediment characteristics explained 57.2% and 35.7% of sediment shear strength and surface stability variance respectively, with organic matter content and season being important factors for both. However large proportions of the variance remained unexplained. Sediments that posed a risk of resuspending high amounts of faecal bacteria could be characterised by season and sediment type, and this should be considered in the future modelling of bathing water quality.
Does Rebound Pain after Peripheral Nerve Block for Orthopedic Surgery Impact Postoperative Analgesia and Opioid Consumption? A Narrative Review
Authors: Dada Olufunke , Gonzalez Zacarias Alicia , Ongaigui Corinna , Echeverria-Villalobos Marco , Kushelev Michael , Bergese Sergio D. , Moran Kenneth
Published: September 2019

Regional anesthesia has been considered a great tool for maximizing post-operative pain control while minimizing opioid consumption. Post-operative rebound pain, characterized by hyperalgesia after the peripheral nerve block, can however diminish or negate the overall benefit of this modality due to a counter-productive increase in opioid consumption once the block wears off. We reviewed published literature describing pathophysiology and occurrence of rebound pain after peripheral nerve blocks in patients undergoing orthopedic procedures. A search of relevant keywords was performed using PubMed, EMBASE, and Web of Science. Twenty-eight articles (n = 28) were included in our review. Perioperative considerations for peripheral nerve blocks and other alternatives used for postoperative pain management in patients undergoing orthopedic surgeries were discussed. Multimodal strategies including preemptive analgesia before the block wears off, intra-articular or intravenous anti-inflammatory medications, and use of adjuvants in nerve block solutions may reduce the burden of rebound pain. Additionally, patient education regarding the possibility of rebound pain is paramount to ensure appropriate use of prescribed pre-emptive analgesics and establish appropriate expectations of minimized opioid requirements. Understanding the impact of rebound pain and strategies to prevent it is integral to effective utilization of regional anesthesia to reduce negative consequences associated with long-term opioid consumption.
Predicting Slope Stability Failure through Machine Learning Paradigms
Authors: Tien Bui Dieu , Moayedi Hossein , Gör Mesut , Jaafari Abolfazl , Foong Loke Kok
Published: September 2019

In this study, we employed various machine learning-based techniques in predicting factor of safety against slope failures. Different regression methods namely, multi-layer perceptron (MLP), Gaussian process regression (GPR), multiple linear regression (MLR), simple linear regression (SLR), support vector regression (SVR) were used. Traditional methods of slope analysis (e.g., first established in the first half of the twentieth century) used widely as engineering design tools. Offering more progressive design tools, such as machine learning-based predictive algorithms, they draw the attention of many researchers. The main objective of the current study is to evaluate and optimize various machine learning-based and multilinear regression models predicting the safety factor. To prepare training and testing datasets for the predictive models, 630 finite limit equilibrium analysis modelling (i.e., a database including 504 training datasets and 126 testing datasets) were employed on a single-layered cohesive soil layer. The estimated results for the presented database from GPR, MLR, MLP, SLR, and SVR were assessed by various methods. Firstly, the efficiency of applied models was calculated employing various statistical indices. As a result, obtained total scores 20, 35, 50, 10, and 35, respectively for GPR, MLR, MLP, SLR, and SVR, revealed that the MLP outperformed other machine learning-based models. In addition, SVR and MLR presented an almost equal accuracy in estimation, for both training and testing phases. Note that, an acceptable degree of efficiency was obtained for GPR and SLR models. However, GPR showed more precision. Following this, the equation of applied MLP and MLR models (i.e., in their optimal condition) was derived, due to the reliability of their results, to be used in similar slope stability problems.
Wind Condition Analysis of Japanese Rural Landscapes in the 19th Century: A Case Study of Kichijoji Village in Musashino Upland
Authors: Natsume Muneyuki
Published: September 2019

Woodlands in the traditional rural landscape of Japan are thought to have a role as windbreaks, among various functions. However, in previous studies, the windbreak effect of woodlands in early-modern settlements has not been quantitatively analyzed. To perform a quantitative analysis, computational fluid dynamics was used with a 3D reconstruction of the early-modern rural landscape of Kichijoji village in a suburb of Tokyo. The landscape was reconstructed based on historical records. The analysis showed that the woodland in Kichijoji village effectively reduced the speed of northbound and southbound winds in the fields. The results are consistent with the actual prevailing wind direction in this area. The purpose of this study was to determine a method and model to quantify the windbreak effect of woodlands in early-modern settlements.
Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)
Authors: Wang Xue-Yang , Wu Kang-Hui , Pang Hui-Lin , Xu Ping-Zhen , Li Mu-Wang , Zhang Guo-Zheng
Published: September 2019

Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens of the silkworm. Cytochrome c (cytc) showed a significant response to BmNPV infection in our previous transcriptome study. However, little is known about the role of Bombyx mori cytc (Bmcytc) in resistance to BmNPV infection. In this study, the expression levels analysis of Bmcytc showed stable expression levels in selected tissues of the resistant strain AN following BmNPV infection, while there was downregulation in the susceptible strain p50, except in the malpighian tubule. To further study the role of Bmcytc in viral infection, Bmcytc was knocked down with siRNA in vitro, resulting in significant downregulation of selected downstream genes of the mitochondrial pathway, including Bmapaf, Bmcaspase-Nc, and Bmcaspase-1; this was also confirmed by overexpression of Bmcytc using the pIZT/V5-His-mCherry insect vector, except Bmcaspase-1. Moreover, knockdown of Bmcytc significantly promoted the infection process of BmNPV in vitro, while the infection was inhibited by overexpression of Bmcytc at the early stage and subsequently increased rapidly. Based on these results, we concluded that Bmcytc plays a vital role in BmNPV infection by regulating the mitochondrial apoptosis pathway. Our work provides valuable data for the clarification of the mechanism of silkworm resistance to BmNPV infection.
RNA-Seq de Novo Assembly and Differential Transcriptome Analysis of Chaga (Inonotus obliquus) Cultured with Different Betulin Sources and the Regulation of Genes Involved in Terpenoid Biosynthesis
Authors: Fradj Narimene , Gonçalves dos Santos Karen Cristine , de Montigny Nicolas , Awwad Fatima , Boumghar Yacine , Germain Hugo , Desgagné-Penix Isabel
Published: September 2019

Chaga (Inonotus obliquus) is a medicinal fungus used in traditional medicine of Native American and North Eurasian cultures. Several studies have demonstrated the medicinal properties of chaga’s bioactive molecules. For example, several terpenoids (e.g., betulin, betulinic acid and inotodiol) isolated from I. obliquus cells have proven effectiveness in treating different types of tumor cells. However, the molecular mechanisms and regulation underlying the biosynthesis of chaga terpenoids remain unknown. In this study, we report on the optimization of growing conditions for cultured I. obliquus in presence of different betulin sources (e.g., betulin or white birch bark). It was found that better results were obtained for a liquid culture pH 6.2 at 28 °C. In addition, a de novo assembly and characterization of I. obliquus transcriptome in these growth conditions using Illumina technology was performed. A total of 219,288,500 clean reads were generated, allowing for the identification of 20,072 transcripts of I. obliquus including transcripts involved in terpenoid biosynthesis. The differential expression of these genes was confirmed by quantitative-PCR. This study provides new insights on the molecular mechanisms and regulation of I. obliquus terpenoid production. It also contributes useful molecular resources for gene prediction or the development of biotechnologies for the alternative production of terpenoids.
Mapping and Identifying a Candidate Gene Plr4, a Recessive Gene Regulating Purple Leaf in Rice, by Using Bulked Segregant and Transcriptome Analysis with Next-Generation Sequencing
Authors: Gao Ju , Dai Gaoxing , Zhou Weiyong , Liang Haifu , Huang Juan , Qing Dongjin , Chen Weiwei , Wu Hao , Yang Xinghai , Li Danting , Gao Lijun , Deng Guofu
Published: September 2019

The anthocyanin biosynthesis of rice is a major concern due to the potential nutritional value. Purple appears in various organs and tissues of rice such as pericarp, flower organs, leaves, leaf sheaths, internodes, ligules, apex, and stigma. At present, there are many studies on the color of rice pericarp, but the gene and mechanism of other organs such as leaves are still unclear, and the gene regulatory network of specific organ coloring has not been systematically understood. In this study, genetic analysis demonstrated that the purple leaf traits of rice were regulated by a recessive gene. The green leaf cultivar Y58S and purple leaf cultivar XianHongB were used to construct the mapping population. A set of near isogenicline (NIL) (BC3F1) was bred via crossing and back-crossing. The generations of BC3F2 appeared to separate four phenotypes, pl1, pl2, pl3, and pl4, due to the occurrence of a purple color in different organs. We constructed three bulked segregant analysis (BSA) pools (pl1–pl2, pl1–pl3, and pl1–pl4) by using the separated generations of BC3F5 and mapped the purple leaf gene plr4 to the vicinity of 27.9–31.1 Mb on chromosome 4. Subsequently, transcriptome sequencing (RNA-Seq) for pl3 and pl2 was used to analyze the differentially expressed genes in the localization interval, where 12 unigenes exhibited differential expression in which two genes (Os04g0577800, Os04g0616400) were downregulated. The two downregulated genes (Os04g0577800 and Os04g0616400) are possible candidate genes because of the recessive genetic characteristics of the purple leaf genes. These results will facilitate the cloning of plr4 and illustrate the molecular mechanisms of the anthocyanin synthesis pathway.
Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBPγ, XBP1, INSM1 and ZNF263
Authors: Khan Rajwali , Raza Sayed Haidar Abbas , Junjvlieke Zainaguli , Xiaoyu Wang , Garcia Matthew , Elnour Ibrahim Elsaeid , Hongbao Wang , Linsen Zan
Published: September 2019

The TORC2 gene is a member of the transducer of the regulated cyclic adenosine monophosphate (cAMP) response element binding protein gene family, which plays a key role in metabolism and adipogenesis. In the present study, we confirmed the role of TORC2 in bovine preadipocyte proliferation through cell cycle staining flow cytometry, cell counting assay, 5-ethynyl-2′-deoxyuridine staining (EdU), and mRNA and protein expression analysis of proliferation-related marker genes. In addition, Oil red O staining analysis, immunofluorescence of adiponectin, mRNA and protein level expression of lipid related marker genes confirmed the role of TORC2 in the regulation of bovine adipocyte differentiation. Furthermore, the transcription start site and sub-cellular localization of the TORC2 gene was identified in bovine adipocytes. To investigate the underlying regulatory mechanism of the bovine TORC2, we cloned a 1990 bp of the 5’ untranslated region (5′UTR) promoter region into a luciferase reporter vector and seven vector fragments were constructed through serial deletion of the 5′UTR flanking region. The core promoter region of the TORC2 gene was identified at location −314 to −69 bp upstream of the transcription start site. Based on the results of the transcriptional activities of the promoter vector fragments, luciferase activities of mutated fragments and siRNAs interference, four transcription factors (CCAAT/enhancer-binding protein C/BEPγ, X-box binding protein 1 XBP1, Insulinoma-associated 1 INSM1, and Zinc finger protein 263 ZNF263) were identified as the transcriptional regulators of TORC2 gene. These findings were further confirmed through Electrophoretic Mobility Shift Assay (EMSA) within nuclear extracts of bovine adipocytes. Furthermore, we also identified that C/EBPγ, XBP1, INSM1 and ZNF263 regulate TORC2 gene as activators in the promoter region. We can conclude that TORC2 gene is potentially a positive regulator of adipogenesis. These findings will not only provide an insight for the improvement of intramuscular fat in cattle, but will enhance our understanding regarding therapeutic intervention of metabolic syndrome and obesity in public health as well.
Inflammatory Response to Different Toxins in Experimental Sepsis Models
Authors: Dickson Kayle , Lehmann Christian
Published: September 2019

Sepsis is defined as life-threatening organ dysfunction caused by the dysregulated host response to infection. Despite serious mortality and morbidity, no sepsis-specific drugs exist. Endotoxemia is often used to model the hyperinflammation associated with early sepsis. This model classically uses lipopolysaccharide (LPS) from Gram-negative pathogens to activate the immune system, leading to hyperinflammation, microcirculatory disturbances and death. Other toxins may also be used to activate the immune system including Gram-positive peptidoglycan (PG) and lipoteichoic acid (LTA). In addition to these standard toxins, other bacterial components can induce inflammation. These molecules activate different signaling pathways and produce different physiological responses which can be taken advantage of for sepsis modeling. Endotoxemia modeling can provide information on pathways to inflammation in sepsis and contribute to preclinical drug development.
Graphene Oxide Nanoparticles Having Long Wavelength Absorbing Chlorins for Highly-Enhanced Photodynamic Therapy with Reduced Dark Toxicity
Authors: Kang Eun Seon , Lee Tae Heon , Liu Yang , Han Ki-Ho , Lee Woo Kyoung , Yoon Il
Published: September 2019

The long wavelength absorbing photosensitizer (PS) is important in allowing deeper penetration of near-infrared light into tumor tissue for photodynamic therapy (PDT). A suitable drug delivery vehicle is important to attain a sufficient concentration of PS at the tumor site. Presently, we developed graphene oxide (GO) nanoparticles containing long wavelength absorbing PS in the form of the chlorin derivative purpurin-18-N-ethylamine (maximum absorption wavelength [λmax] 707 nm). The GO–PS complexes comprised a delivery system in which PS was loaded by covalent and noncovalent bonding on the GO nanosheet. The two GO–PS complexes were fully characterized and compared concerning their synthesis, stability, cell viability, and dark toxicity. The GO–PS complexes produced significantly-enhanced PDT activity based on excellent drug delivery effect of GO compared with PS alone. In addition, the noncovalent GO–PS complex displayed higher photoactivity, corresponding with the pH-induced release of noncovalently-bound PS from the GO complex in the acidic environment of the cells. Furthermore, the noncovalently bound GO‒PS complex had no dark toxicity, as their highly organized structure prevented GO toxicity. We describe an excellent GO complex-based delivery system with significantly enhanced PDT with long wavelength absorbing PS, as well as reduced dark toxicity as a promising cancer treatment.
An Optimised Compaction Process for Zr-Fumarate (MOF-801)
Authors: Taddei Marco , McPherson Matthew J. , Gougsa Abel , Lam Jamie , Sewell Jack , Andreoli Enrico
Published: September 2019

We reported a systematic approach aimed at identifying the optimal conditions for compaction of MOF-801, a small-pore zirconium-based metal–organic framework (MOF) containing fumaric acid as the linker, that can be easily synthesised in aqueous medium. Pellets of the MOF were prepared by compressing the powder either in neat form or dry-mixed with binders (sucrose, polyvinylalcohol, polyvinylbutyral) under a range of pressures and for different times. The mechanical stability and durability of the pellets was tested by simple drop tests and shake tests, finding that addition of 5% of polyvinylbutyral was enough to produce highly resilient pellets that did not release significant amounts of powder upon cracking. The crystallinity, textural properties and CO2 adsorption performance of the MOF were successively assessed, observing the least change of the original properties in pellets compressed at 146 MPa for 15 s. Compaction at higher pressures impacted the performance more heavily, with no evident benefit from the mechanical point of view, whereas compression time did not have a relevant effect. The cyclic adsorption behaviour was tested, showing that the pellets retained as much as 90% of the CO2 working capacity, while displaying unaffected sorption kinetics, and 74% of the H2O working capacity.
Extrusion-Based Additive Manufacturing of Concrete Products: Revolutionizing and Remodeling the Construction Industry
Authors: Valente Marco , Sibai Abbas , Sambucci Matteo
Published: September 2019

Additive manufacturing is one of the main topics of the fourth industrial revolution; defined as Industry 4.0. This technology offers several advantages related to the construction and architectural sectors; such as economic; environmental; social; and engineering benefits. The usage of concrete in additive technologies allows the development of innovative applications and complexity design in the world of construction such as buildings; housing modules; bridges; and urban and domestic furniture elements. The aim of this review was to show in detail a general panoramic of extrusion-based additive processes in the construction sector; the main advantages of using additive manufacturing with the respect to traditional manufacturing; the fundamental requirements of 3D printable material (fresh and hardened properties), and state-of-the-art aesthetic and architectural projects with functional properties.
Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography
Authors: Jeon Deokmin , Kim Joon Ki , Jeon Mansik , Kim Jeehyun
Published: September 2019

Optical coherence tomography (OCT) has a micro-resolution with a penetration depth of about 2 mm and field of view of about 10 mm. This makes OCT well suited for analyzing the anatomical and internal structural assessment of the middle ear. To study the vibratory motion of the tympanic membrane (TM) and its internal structure, we developed a phase-resolved Doppler OCT system using Kasai’s autocorrelation algorithm. Doppler optical coherence tomography is a powerful imaging tool which can offer the micro-vibratory measurement of the tympanic membrane and obtain the micrometer-resolved cross-sectional images of the sample in real-time. To observe the relative vibratory motion of individual sections (malleus, thick regions, and the thin regions of the tympanic membrane) of the tympanic membrane in respect to auditory signals, we designed an experimental study for measuring the difference in Doppler phase shift for frequencies varying from 1 to 8 kHz which were given as external stimuli to the middle ear of a small animal model. Malleus is the very first interconnecting region between the TM and cochlea. In our proposed study, we observed that the maximum change in Doppler phase shift was seen for the 4 kHz acoustic stimulus in the malleus, the thick regions, and in the thin regions of the tympanic membrane. In particular, the vibration signals were higher in the malleus in comparison to the tympanic membrane.
The Influence of Free Long Wave Generation on the Shoaling of Forced Infragravity Waves
Authors: Moura Theo , Baldock Tom
Published: September 2019

Different conceptual models for forced infragravity (long) waves exist in the literature, which suggest different models for the behavior of shoaling forced waves and the possible radiation of free long waves in that process. These are discussed in terms of existing literature. A simple numerical model is built to evaluate the wave shape in space and time during shoaling of forced waves with concurrent radiation of free long waves to ensure mass continuity. The same qualitative results were found when performing simulations with the COULWAVE model using the radiation stress term in the momentum equation to force the generation and propagation of bound waves. Both model results indicate a strong frequency dependence in the shoaling rate and on the lag of the total long wave with respect to the forcing, consistent with observations in the literature and more complex evolution models. In this approach, a lag of the long wave is only observed in the time domain, not in the space domain. In addition the COULWAVE is used to investigate dissipation rates of incident free and forced long waves inside the surf zone. The results also show a strong frequency dependence, as previously suggested in the literature, which can contribute to the total rate of decay of the incident forced wave after short wave breaking.
Defects, Diffusion, and Dopants in Li2Ti6O13: Atomistic Simulation Study
Authors: Kuganathan Navaratnarajah , Ganeshalingam Sashikesh , Chroneos Alexander
Published: September 2019

In this study, force field-based simulations are employed to examine the defects in Li-ion diffusion pathways together with activation energies and a solution of dopants in Li2Ti6O13. The lowest defect energy process is found to be the Li Frenkel (0.66 eV/defect), inferring that this defect process is most likely to occur. This study further identifies that cation exchange (Li–Ti) disorder is the second lowest defect energy process. Long-range diffusion of Li-ion is observed in the bc-plane with activation energy of 0.25 eV, inferring that Li ions move fast in this material. The most promising trivalent dopant at the Ti site is Co3+, which would create more Li interstitials in the lattice required for high capacity. The favorable isovalent dopant is the Ge4+ at the Ti site, which may alter the mechanical property of this material. The electronic structures of the favorable dopants are analyzed using density functional theory (DFT) calculations.
Alumina-Doped Zirconia Submicro-Particles: Synthesis, Thermal Stability, and Microstructural Characterization
Authors: Dahl Gregor Thomas , Döring Sebastian , Krekeler Tobias , Janssen Rolf , Ritter Martin , Weller Horst , Vossmeyer Tobias
Published: September 2019

Zirconia nanoceramics are interesting materials for numerous high-temperature applications. Because their beneficial properties are mainly governed by the crystal and microstructure, it is essential to understand and control these features. The use of co-stabilizing agents in the sol-gel synthesis of zirconia submicro-particles should provide an effective tool for adjusting the particles’ size and shape. Furthermore, alumina-doping is expected to enhance the particles’ size and shape persistence at high temperatures, similar to what is observed in corresponding bulk ceramics. Dispersed alumina should inhibit grain growth by forming diffusion barriers, additionally impeding the martensitic phase transformation in zirconia grains. Here, alumina-doped zirconia particles with sphere-like shape and average diameters of ∼ 300 n m were synthesized using a modified sol-gel route employing icosanoic acid and hydroxypropyl cellulose as stabilizing agents. The particles were annealed at temperatures between 800 and 1200 ∘ C and characterized by electron microscopy, elemental analysis, and X-ray diffraction. Complementary elemental analyses confirmed the precise control over the alumina content (0–50 mol%) in the final product. Annealed alumina-doped particles showed more pronounced shape persistence after annealing at 1000 ∘ C than undoped particles. Quantitative phase analyses revealed an increased stabilization of the tetragonal/cubic zirconia phase and a reduced grain growth with increasing alumina content. Elemental mapping indicated pronounced alumina segregation near the grain boundaries during annealing.
Influence of Fragment Size on the Time and Temperature of Ethylene Vinyl Acetate Lamination Decomposition in the Photovoltaic Module Recycling Process
Authors: Kuczyńska-Łażewska Anna , Klugmann-Radziemska Ewa
Published: September 2019

Photovoltaics is a commercially available and reliable technology with significant potential for long-term growth in nearly all global regions. Several research institutes and companies are working on recycling concepts for thin film modules and modules with crystalline cells. The establishment of recycling and reuse technologies appropriate and applicable to all photovoltaics (PV) modules is a key issue to be addressed as part of corporate social responsibility to safeguard the environment and to implement a fully material-circulated society without any waste. The copolymer ethylene-vinyl acetate (EVA) layer is a thermoplastic containing cross-linkable ethylene vinyl acetate, which is used to encapsulate the photovoltaic cells. The cells are laminated between films of EVA in a vacuum, under compression, and up to 150·°C. The encapsulant’s primary purpose is to bond or laminate the multiple layers of the module together. In the photovoltaic module recycling process, the second important step (after mechanical dismantling of the frame) is EVA lamination removal. In this study, different parameters of the thermal delamination method used during the recycling process were experimentally tested and compared, and the most ecological and economical one is proposed.
Effect of Nanoparticle Content on the Microstructural and Mechanical Properties of Forged and Heat-Treated TiC/2219 Nanocomposites
Authors: Yang Yilong , Jiang Ripeng , Li Xiaoqian , Zhang Yun , Li Ruiqing
Published: September 2019

In this study, castings of TiC nanoparticle reinforced 2219 aluminum matrix composites with different TiC nanoparticle contents (0, 0.5, 0.9, 1.3, and 1.7 wt.%) prepared using an ultrasound-assisted stirring technology were deformed by multidirectional forging at 510 °C followed by T6 aging treatment. The microstructural evolution and mechanical properties of the 2219 alloy and its composites were investigated and compared. Optical microscopy and scanning electron microscopy revealed that the composite with 0.9 wt.% TiC nanoparticle content possessed finer grains and the lowest amount of Al2Cu phases. The electron backscattered diffraction (EBSD) was used to characterize the sub-grains. The precipitation microstructures of the 2219 alloy and composites with different nanoparticle contents were characterized by differential scanning calorimetry and transmission electron microscopy. It was found that 0.9 wt.% TiC/2219 nanocomposites contained the highest amount of θ″ and θ′ phases with shorter lengths. This might imply that the nanoparticles uniformly dispersed in the matrix could facilitate the precipitation of θ″ and θ′ phases during aging. Thus, the 0.9 wt.% TiC/2219 nanocomposite showed the best mechanical properties. The ultimate tensile strength, yield strength, and elongation of the 0.9 wt.% TiC/2219 nanocomposite increased by 24.2, 46.1, and 37.2%, respectively, compared to those of the 2219 alloy.
Erratum: Chen, F. et al. Advances of Metabolomics in Fungal Pathogen–Plant Interactions. Metabolites 2019, 9, 169
Authors: Chen Fangfang , Ma Ruijing , Chen Xiao-Lin
Published: September 2019

The authors wish to make the following corrections to this paper [...]
Probiotic and Triticale Silage Fermentation Potential of Pediococcus pentosaceus and Lactobacillus brevis and Their Impacts on Pathogenic Bacteria
Authors: Soundharrajan Ilavenil , Kim Dahye , Kuppusamy Palaniselvam , Muthusamy Karanan , Lee Hyun Jeong , Choi Ki Choon
Published: September 2019

The purpose of this study was to identify potent lactic acid bacteria that could have a great impact on triticale silage fermentation at different moisture levels and determine their anti-bacterial activity and high probiotic potential. For this purpose, Pediococcus pentosaceus (TC48) and Lactobacillus brevis (TC50) were isolated from fermented triticale silage. The fermentation ability of these isolates in triticale powder was studied by an ensiling method. TC48 had higher ability to ferment silage powder by increasing the lactic acid content of silage than TC50. Extracellular supernatant (ECS) of TC48 and TC50 exhibited strong antibacterial effects (inhibition zone diameters: 18–28 mm) against tested cattle pathogenic bacteria with minimum inhibitory/ minimum bactericidal concentrations (MIC/MBC) values of 5.0–10 mg/mL and 10–20 mg/mL, respectively. Extracellular supernatant (ECS) of TC48 and TC50 showed antibacterial activities on E. coli, P. aeruoginosa, S. aureus and E. faecalis through destruction of membrane integrity as confirmed by decreased viability, and increased 260 nm absorbing material in culture filtrate of pathogenic bacteria exposed to ECS of both strains. TC48 and TC50 strains exhibited high tolerance to artificial gastric, duodenal and intestinal fluids. TC48 showed good hydrophobicity and auto-aggregations properties. TC48 and TC50 significantly co-aggregated with E. coli, P. aeruoginosa, S. aureus and E. faecalis in a time-dependent manner. In summary, all of the bacteria had a positive impact on at least one functional property of the silage during the fermentation process. However, the addition of P. pentosaceus (TC48) and L. brevis (TC50) yielded the greatest silage quality improvement, having high antibacterial and probiotic properties.
Molecular Dynamics Gives New Insights into the Glucose Tolerance and Inhibition Mechanisms on β-Glucosidases
Authors: Costa Leon Sulfierry Corrêa , Mariano Diego César Batista , Rocha Rafael Eduardo Oliveira , Kraml Johannes , Silveira Carlos Henrique da , Liedl Klaus Roman , de Melo-Minardi Raquel Cardoso , Lima Leonardo Henrique Franca de
Published: September 2019

β-Glucosidases are enzymes with high importance for many industrial processes, catalyzing the last and limiting step of the conversion of lignocellulosic material into fermentable sugars for biofuel production. However, β-glucosidases are inhibited by high concentrations of the product (glucose), which limits the biofuel production on an industrial scale. For this reason, the structural mechanisms of tolerance to product inhibition have been the target of several studies. In this study, we performed in silico experiments, such as molecular dynamics (MD) simulations, free energy landscape (FEL) estimate, Poisson–Boltzmann surface area (PBSA), and grid inhomogeneous solvation theory (GIST) seeking a better understanding of the glucose tolerance and inhibition mechanisms of a representative GH1 β-glucosidase and a GH3 one. Our results suggest that the hydrophobic residues Y180, W350, and F349, as well the polar one D238 act in a mechanism for glucose releasing, herein called “slingshot mechanism”, dependent also on an allosteric channel (AC). In addition, water activity modulation and the protein loop motions suggest that GH1 β-Glucosidases present an active site more adapted to glucose withdrawal than GH3, in consonance with the GH1s lower product inhibition. The results presented here provide directions on the understanding of the molecular mechanisms governing inhibition and tolerance to the product in β-glucosidases and can be useful for the rational design of optimized enzymes for industrial interests.
Identification of Passion Fruit Oil Adulteration by Chemometric Analysis of FTIR Spectra
Authors: Kiefer Johannes , Lampe Anja I. , Nicoli Stefano F. , Lucarini Massimo , Durazzo Alessandra
Published: September 2019

Passion fruit oil is a high-value product with applications in the food and cosmetic sectors. It is frequently diluted with sunflower oil. Sunflower oil is also a potential adulterant as its addition does not notably alter the appearance of the passion fruit oil. In this paper, we show that this is also true for the FTIR spectrum. However, the chemometric analysis of the data changes this situation. Principal component analysis (PCA) enables not only the straightforward discrimination of pure passion fruit oil and adulterated samples but also the unambiguous classification of passion fruit oil products from five different manufacturers. Even small amounts—significantly below 1%—of the adulterant can be detected. Furthermore, partial least-squares regression (PLSR) facilitates the quantification of the amount of sunflower oil added to the passion fruit oil. The results demonstrate that the combination of FTIR spectroscopy and chemometric data analysis is a very powerful tool to analyze passion fruit oil.