bluuebunny/crossref_metadata_2025 huggingface dataset:
Patient Safety Culture: Nurses’ Perspective in the Hospital Setting
Authors: Reyes Ramos Maria José , Costa Abós Silvia
Published: May 2024

(1) Background: Patient safety culture (PSC) encompasses the values, attitudes, norms, beliefs, practices, perceptions, competencies, policies, and behaviours of professionals that determine organisational commitment to quality and patient safety. Few studies use mixed methods to analyse patient safety culture, and none offer the richness of using a mixed methodology to develop their theoretical model. This study aims to identify the factors nurses believe contextualise and influence PSC in relation to existing theoretical frameworks. (2) Methods: This study employed a sequential explanatory mixed-methods design combined with the Pillar Integration Process for data integration. (3) Results: In the final data integration process, 26 factors affecting nurses’ PSC were identified. Factors nurses related to PSC not being assessed with the tool used in phase 1 were notification system, flow of patients, patient involvement, resources and infrastructure, and service characteristics. (4) Conclusions: This mixed-methods study provides an opportunity to identify the weaknesses and strengths of currently developed theoretical frameworks related to PSC and offers content for its improvement. Even though multiple studies aim to assess PSC using existing quantitative method tools, the development of this study offers a glimpse of some aspects relevant to nurses’ PSC not included in the theoretical framework of the said tools, such as patient involvement, the flow of patients, and service infrastructure.
Digital Training for Nurses and Midwives to Improve Treatment for Women with Postpartum Depression and Protect Neonates: A Dynamic Bibliometric Review Analysis
Authors: Tzitiridou-Chatzopoulou Maria , Orovou Eirini , Zournatzidou Georgia
Published: May 2024

The high prevalence of postpartum depression makes it necessary for midwives and nurses to implement prenatal interventions for expectant mothers. The current study aims to investigate and highlight the importance of the digital training of nurses in order to help women mitigate the symptoms of postpartum depression and protect infants. To approach this, we conducted a bibliometric analysis to address the study’s main objective. Articles were retrieved from the Scopus database for the timeframe 2000–2023. Data analysis was conducted using the statistical programming language R (version R-4.4.) and the bibliometric software VOSviewer (version 1.6.20) and Biblioshiny (version 4.1.4), focused on year, journal, and country. For this investigation, we selected a total of 31 MeSH keywords and sub-headings that exhibited significant frequencies. We consistently used six significant clusters of MeSH keywords. We obtained a total of 585 articles from the Scopus database that were major contributors to the field of PPD, as evidenced by their extensive publication of research articles and their influential role in the domain. The studies included a thorough analysis of depression research, the use of scales for diagnosing and screening PPD, psychological studies related to PPD, and the exploration of causes, mechanisms, outcomes, and genetic factors. Our study’s results demonstrate a steady and significant increase in the availability of information on PPD. Importantly, the novelty of the current study lies in highlighting the need for a transition in the ways in which nurses and midwives are trained to mitigate postpartum disease by integrating emerging technologies into their practices. The knowledge provided here has the potential to serve as a foundation for future advancements in obstetric psychology, both presently and in the future.
Prevalence of Liver Steatosis in Tuberous Sclerosis Complex Patients: A Retrospective Cross-Sectional Study
Authors: De Bock Thaïs , Brussaard Carola , François Silke , François Karlien , Seynaeve Laura , Jansen Anna , Wissing Karl Martin , Janssens Peter
Published: May 2024

Introduction: Tuberous sclerosis complex (TSC) is a genetic disease caused by pathogenetic variants in either the TSC1 or TSC2 genes. Consequently, the mechanistic target of the rapamycin complex 1 (mTORC1) pathway, a regulator of cell growth, metabolism, and survival, becomes inappropriately activated, leading to the development of benign tumors in multiple organs. The role of mTORC1 in lipid metabolism and liver steatosis in TSC patients has not been well-studied, and clinical data on liver involvement in this population are scarce. Methods: We conducted a retrospective, cross-sectional study to compare liver steatosis in TSC patients with age-, sex-, BMI-, and diabetes status-matched controls. Participants with a definite diagnosis of TSC were recruited from the TSC clinic at UZ Brussel. Liver steatosis was quantified using the fat signal fraction from in-phase and out-of-phase MRI, with a threshold of ≥5% defining the presence of steatosis. We also evaluated the prevalence of liver angiomyolipomata in the TSC group and analyzed risk factors for both liver steatosis and angiomyolipomata. Results: The study included 59 TSC patients and 59 matched controls. The mean fat signal fraction was 4.0% in the TSC group and 3.9% in the controls, showing no significant difference (two-tailed Wilcoxon signed ranks test, p = 0.950). Liver steatosis was observed in 15.3% of TSC patients compared to 23.7% of the controls, which was not statistically significant (two-tailed McNemar test, p = 0.267). Liver angiomyolipomata were identified in 13.6% of the TSC cohort. Conclusions: Our study, describing in detail the liver phenotype of TSC patients, did not reveal a significant difference in the prevalence of MRI-assessed liver steatosis in a large cohort of TSC patients compared to a closely matched control group.
Three-Dimensional Cone Beam Computed Tomography (CBCT)-Derived Soft Tissue Changes in Patients with Unilateral Cleft Lip, Alveolus, and Palate with Midfacial Deficiency after 1.5 Years of Bone-Anchored Maxillary Protraction
Authors: Steegman Ralph M. , Klein Meulekamp Annemarlien F. , Renkema Anne-Marie , Janssen Krista I. , Kuijpers-Jagtman Anne Marie , Ren Yijin
Published: May 2024

Background: Bone-anchored maxillary protraction (BAMP) aims to correct midfacial deficiencies, with proven positive skeletal changes without potential unwanted side effects. However, the influence of BAMP treatment on facial soft tissues, particularly in subjects with complete unilateral cleft lip, alveolus, and palate (CUCLAP), remains unclear. Methods: This single-center longitudinal cohort study examined the effects of 1.5 years of BAMP treatment on facial soft tissues in growing subjects with complete unilateral cleft lip, alveolus, and palate. The sample consisted of 25 patients, age range 9.7 to 12.6 years. Three-dimensional surface models derived from CBCT scans were superimposed on stable structures of the anterior cranial base and on the occipital area posterior of the foramen magnum to assess three-dimensional changes due to growth and BAMP therapy. Results: The results revealed a moderate positive correlation (Pearson’s correlation coefficient from 0.203 to 0.560) between changes in hard tissue and soft tissue; some correlations were found to be weak (<0.300). Linear changes in soft tissue following BAMP were in the same direction as skeletal changes, showing downward, forward, and outward displacement. The only exception was in the vertical dimension. The lower facial third showed a slight but significant reduction, mainly in lip length (−1.2 mm), whereas the middle facial third showed a small increase (1.1 mm). Conclusions: It was concluded that during BAMP, soft tissue changes occur in the same direction as skeletal changes, although with a larger variability and less pronounced effects.
Analysis of Target Vessel Instability in Fenestrated Endovascular Repair (f-EVAR) in Thoraco-Abdominal Aortic Pathologies
Authors: Becker Daniel , Sikman Laura , Ali Ahmed , Mosbahi Selim , F. Prendes Carlota , Stana Jan , Tsilimparis Nikolaos
Published: May 2024

Objective: The aim of this study was to evaluate the influence of target vessel anatomy and post-stenting geometry on the outcome of fenestrated endovascular aortic repair (f-EVAR). Methods: A retrospective review of data from a single center was conducted, including all consecutive fenestrated endovascular aortic repairs (f-EVARs) performed between September 2018 and December 2023 for thoraco-abdominal aortic aneurysms (TAAAs) and complex abdominal aortic aneurysms (cAAAs). The analysis focused on the correlation of target vessel instability to target vessel anatomy and geometry after stenting. The primary endpoint was the cumulative incidence of target vessel instability. Secondary endpoints were the 30-day and follow-up re-interventions. Results: A total of 136 patients underwent f-EVAR with 481 stented target vessels. A total of ten target vessel instabilities occurred including three in visceral and seven instabilities in renal vessels. The cumulative incidence of target vessel instability with death as the competing risk was 1.4%, 1.8% and 3.4% at 1, 2 and 3 years, respectively. In renal target vessels (260/481), a diameter ≤ 4 mm (OR 1.21, 95% CI 1.035–1.274, p = 0.009) and an aortic protrusion ≥ 5.75 mm (OR 8.21, 95% CI 3.150–12-23, p = 0.027) was associated with an increased target vessel instability. In visceral target vessels (221/481), instability was significantly associated with a preoperative tortuosity index ≥ 1.25 (HR 15.19, CI 95% 2.50–17.47, p = 0.045) and an oversizing ratio of ≥1.25 (HR 7.739, CI % 4.756–12.878, p = 0.049). Conclusions: f-EVAR showed favorable mid-term results concerning target vessel instability in the current cohort. A diameter of ≤4 mm and an aortic protrusion of ≥5.75 mm in the renal target vessels as well as a preoperative tortuosity index and an oversizing of the bridging stent of ≥1.25 in the visceral target vessels should be avoided.
Untangling Structural and Functional Diversity of Prokaryotic Microbial Assemblage on Mangrove Pneumatophores
Authors: Bohra Varsha , Tam Nora Fung-Yee , Chen Luzhen , Lai Kaze King-Yip , Lam Winnie , Xu Steven Jing-Liang , Zhou Hai-Chao , Lang Tao , Lee Chak-Lam , Lee Fred Wang-Fat
Published: May 2024

Mangroves are important coastal wetlands along tropical and subtropical regions. Pneumatophore, a kind of aerial root, is among the prominent components of a mangrove ecosystem, which provides microhabitats for a range of prokaryotic (bacteria and cyanobacteria) microbial assemblages, whose role in the maintenance of mangrove ecology often remains neglected. Very few studies are available on pneumatophore-associated prokaryotic microorganisms (PAPMs). The majority of them are related to the microscopic identification of cyanobacteria, with very limited research on the bacterial population, even though they demand more attention. Also, very scarce information is available on biotic and abiotic factors shaping the PAPMs. The objective of this review is to highlight the structural and functional importance of prokaryotic organisms associated with pneumatophores. This review begins with a brief introduction of what mangrove pneumatophores are, then focuses on the PAPMs, accentuating the breadth and depth of information gained from previous research. We further discuss how a combination of a traditional cultivable approach and a newly developed omics approach can be efficaciously employed to untangle PAPMs. This review provides updated information on PAPMs, which will intensify the visibility and necessity of pneumatophore-associated microbial community research.
A Narrative Review of Revascularization in Chronic Coronary Syndrome/Disease: Concepts and Misconceptions
Authors: Nogueira-Garcia Beatriz , Vilela Marta , Oliveira Catarina , Caldeira Daniel , Martins Ana Margarida , Nobre Menezes Miguel
Published: May 2024

Ischemic heart disease represents a significant global burden of morbidity and mortality. While revascularization strategies are well defined in acute settings, there are uncertainties regarding chronic coronary artery disease treatment. Recent trials have raised doubts about the necessity of revascularization for “stable”, chronic coronary syndromes or disease, leading to a shift towards a more conservative approach. However, the issue remains far from settled. In this narrative review, we offer a summary of the most pertinent evidence regarding revascularization for chronic coronary disease, while reflecting on less-often-discussed details of major clinical trials. The cumulative evidence available indicates that there can be a prognostic benefit from revascularization in chronic coronary syndrome patients, provided there is significant ischemia, as demonstrated by either imaging or coronary physiology. Trials that have effectively met this criterion consistently demonstrate a reduction in rates of spontaneous myocardial infarction, which holds both prognostic and clinical significance. The prognostic benefit of revascularization in patients with heart failure with reduced ejection fraction remains especially problematic, with a single contemporary trial favouring surgical revascularization. The very recent publication of a trial focused on revascularizing non-flow-limiting “vulnerable” plaques adds further complexity to the field. The ongoing debates surrounding revascularization in chronic coronary syndromes emphasize the importance of personalized strategies. Revascularization, added to the foundational pillar of medical therapy, should be considered, taking into account symptoms, patient preferences, coronary anatomy and physiology, ischemia tests and intra-coronary imaging.
How Did COVID-19 Pandemic Stress Affect Poles’ Views on the Role of the Forest?
Authors: Janeczko Emilia , Banaś Jan , Woźnicka Małgorzata , Janeczko Krzysztof , Utnik-Banaś Katarzyna , Zięba Stanisław , Fialova Jitka
Published: May 2024

The 2020–2021 COVID-19 pandemic has had a tremendous impact on the daily lives of everyone, including local communities and entire societies. Under the influence of this new experience, the importance of the services and benefits provided by forests and other green spaces has increased. A very large role in this aspect was played by media messages promoting the idea of being close to nature as a remedy for malaise and stress, and pushing the thesis that the risk of spreading the virus outdoors is lower than it is indoors. Thanks to media messages, as well as government responses (i.e., lockdown, temporary bans on entering the forest), public attention has been directed toward forests, generating greater interest in forest management and conservation issues, as well as in nature and forestry education. The purpose of our research was to determine how the pandemic affected the frequency of visits to the forest and how it changed the public’s views on the role of forests. The research material consists of the results of a questionnaire survey (online and traditionally way) carried out in Poland from September to October in 2020. A total of 1402 people were surveyed. The results show that nearly 52% of respondents increased their use of forest recreational services during the COVID-19 pandemic. We also found that more than 80% of respondents agreed with statements that the forest is a safer space than, for example, parks or squares, and it is more difficult to contract the virus there. Men were more likely to agree with this statement than women (1.51), respondents without children (1.45), respondents over the age of 31 (1.72), and respondents with more than primary or secondary education (1.37). Also, more than 80% of respondents said that the social functions of the forest (e.g., recreational) had gained importance as a result of the pandemic. The social functions of the forest gained importance primarily among respondents with higher education (2.40), and among respondents who had visited the forest rather infrequently (several times a year) for recreational purposes before the pandemic (1.72). Those with children were more likely to agree with the statement that the economic functions of the forest have lost their importance (1.43), as were those who had formerly visited the forest several times a year (1.53). With regard to the statement “the slowdown of the economy has contributed to the improvement of the environment,” there were no statistically significant differences in the views of respondents in terms of their socio-demographic characteristics.
“Architectural Acupuncture” in Urban Morphology Studies
Authors: Carlotti Paolo
Published: May 2024

This paper aims to track the evolution of urban morphology studies, focusing on a graphical understanding of transformation phenomena in historical and contemporary city fabrics. It points out similarities in urban morphology studies by authors like Aldo Rossi, Carlo Oswald W. Ungers, Hans Kollhoff, Saverio Muratori, Gianfranco Caniggia, and Giancarlo de Carlo. These studies developed within a supportive cultural environment, aligning with analogical procedures and anticipating the contemporary concept of urban acupuncture. Urban acupuncture denotes episodic and locally impactful interventions countering grand, self-celebratory architectural projects. These interventions are promoted both by liberal and capitalist culture as well as by socialist-inspired culture. Lastly, these interventions, promoted by various cultural backgrounds, highlight the multi-scale nature of urban morphology studies and urban acupuncture projects. Each change in form corresponds to a morphological adaptation and a redefinition of urban rules and grammar usable in projects with territorial significance. Today, enhanced by digital tools, these studies confirm insights and syntheses, presenting urban acupuncture interventions in real-time socio-economic flows and dynamics.
The Relationship between Farmland Abandonment and Urbanization Processes: A Case Study in Four Chinese Urban Agglomerations
Authors: Zheng Nan , Li Le , Han Lijian , Zhu Xiufang , Zhao Kefei , Zhu Ziyang , Ye Xiaolan
Published: May 2024

Clarifying the relationship between urbanization and farmland abandonment in urban agglomerations (UAs) is crucial to guide the formulation of arable land management policies and strategies for sustainable urban development. Despite numerous studies confirming the correlation between farmland abandonment and certain urbanization factors, the exploration of the patterns and underlying mechanisms of farmland abandonment in China’s UAs remains worthy of systematic investigation. In this study, we conducted an analysis of the spatiotemporal trends in farmland abandonment and examined the key drivers of farmland abandonment in four representative Chinese UAs—Beijing–Tianjin–Hebei (BTH), Chengdu–Chongqing (CC), Pearl River Delta (PRD), and Yangtze River Delta (YRD). Our findings reveal that farmland abandonment has been intensified with increasing fragmentation and aggregation patches across these UAs. Abandonment experience was the main driver of continuous abandonment. Moreover, natural conditions persistently influenced farmland abandonment in the BTH, while land urbanization and economic urbanization were predominant drivers in the CC. The abandonment in the PRD was mainly driven by population urbanization, while the abandonment in the YRD was primarily driven by economic urbanization and land urbanization. The research findings provide data support and scientific explanation for land policy-making in these typical UAs under different development strategies.
Can Market-Oriented Allocation of Land Factors Promote the Adoption of Cropland Quality Protection Behaviors by Farmers: Evidence from Rural China
Authors: Shen Lulin , Wang Fang
Published: May 2024

Protecting cropland quality is a fundamental national policy that China must adhere to for the long term. This study examines the impact of market-oriented allocation of land factors on farmers’ cropland quality protection behaviors and its mechanism of action, based on survey data from 3804 farm households in the 2020 China Rural Revitalization Survey (CRRS). The study employs the Ordered Probit (O-probit) model, the mediated effect model, and other econometric tools to analyze the data. The study found that the market-oriented allocation of land factors can significantly promote farmers’ adoption of cropland quality protection behaviors. The robustness test supports this conclusion. The market-oriented allocation of land factors indirectly promotes the adoption of cropland quality protection by expanding the plot size and improving agricultural income. The analysis of heterogeneity indicates that farmers are more likely to adopt cropland quality protection behaviors in the plains, suburban areas, or areas with better developed labor markets. Therefore, it is essential to continue promoting market-oriented reforms of rural land factors, actively promoting land transfer policies, and guiding the development of agricultural operations towards scaling, specialization, and modernization. This will achieve the rational allocation of land resources. It is important to consider geographical variations in each area when implementing policies to guarantee effective utilization and protection of cropland.
Spatial Distribution of Relationship between Historical Monuments and Tourism: The Case Study of Bihor County in Romania
Authors: Herman Grigore Vasile , Blaga Lucian , Filimon Claudiu , Caciora Tudor , Filimon Luminița , Herman Laura Mariana , Wendt Jan A.
Published: May 2024

Tourism is one of the emerging branches of the economy, playing an important role in the development of specific economies within local communities. In this context, the perspectives of exploiting historical monuments, seen as raw material in the tourism industry, represent a desirable goal worth considering at the locality and territorial administrative unit level. The purpose of this study is to highlight the relationship between historical monuments, viewed as factors generating tourist motivation and tourism. This was made possible by conducting a spatial analysis (at the level of territorial administrative units and localities) of the defining criteria for historical monuments and tourism in Bihor County, Romania. The research methodology involved the use of multicriteria analysis to identify and establish the types of relationships between historical monuments and tourism, at a spatial level. The results of the study aimed to present an image of the spatial distribution of the characteristics of historical monuments and tourism, as well as to establish and depict spatial relationships between them, thus partially confirming the working hypothesis that the number and importance of historical monuments influence and determine tourist activity within a given area. Thus, although the studied area has 455 historical monuments, they are not exploited from a tourist point of view, with there being no strong relationships, except at the level of 19 territorial administrative units (18.8%), respectively, in 15 localities (3.3%). Among them, the obtained values stand out for the territorial administrative units of Oradea and Biharia, respectively, in the localities of Oradea and Beiuș.
Spatio-Temporal Evolution and Multi-Scenario Simulation of Non-Grain Production on Cultivated Land in Jiangsu Province, China
Authors: Jiang Chengge , Wang Lingzhi , Guo Wenhua , Chen Huiling , Liang Anqi , Sun Mingying , Li Xinyao , Omrani Hichem
Published: May 2024

Cultivated land plays a crucial role as the basis of grain production, and it is essential to effectively manage the unregulated expansion of non-grain production (NGP) on cultivated land in order to safeguard food security. The study of NGP has garnered significant attention from scholars, but the prediction of NGP trends is relatively uncommon. Therefore, we focused on Jiangsu Province, a significant grain production region in China, as the study area. We extracted data on cultivated land for non-grain production (NGPCL) in 2000, 2005, 2010, 2015, and 2019, and calculated the ratio of non-grain production (NGPR) for each county unit in the province. On this basis, Kernel Density Estimation (KDE) and spatial autocorrelation analysis tools were utilized to uncover the spatio-temporal evolution of NGP in Jiangsu Province. Finally, the Patch-Generating Land Use Simulation (PLUS) model was utilized to predict the trend of NGP in Jiangsu Province in 2038 under the three development scenarios of natural development (NDS), cultivated land protection (CPS), and food security (FSS). After analyzing the results, we came to the following conclusions:(1) During the period of 2000–2019, the NGPCL area and NGPR in Jiangsu Province exhibited a general decreasing trend. (2) The level of NGP displayed a spatial distribution pattern of being “higher in the south and central and lower in the north”. (3) The results of multi-scenario simulation show that under the NDS, the area of NGPCL and cultivated land for grain production (GPCL) decreases significantly; under the CPS, the decrease in NGPCL and GPCL is smaller than that of the NDS. Under the FSS, NGPCL decreases, while GPCL increases. These results can provide reference for the implementation of land use planning, the delineation of the cultivated land protection bottom line, and the implementation of thee cultivated land use control system in the study area.
Farmland Rental Market, Outsourcing Services Market and Agricultural Green Productivity: Implications for Multiple Forms of Large-Scale Management
Authors: Zhang Heng , Guo Xiangyu
Published: May 2024

Large-scale management is the key to realizing long-term agricultural growth in smallholder countries. Land-scale management and service-scale management are two forms of agricultural large-scale management. The former is committed to changing the small-scale management pattern, but the latter tends to maintain it. There has been a lack of discussion and controversy about the relationship between the two. From the perspective of market maturity, this paper explores whether the two are complementary or mutually exclusive and how their complementary or mutually exclusive relationship affects agricultural green productivity. The results show the following: Land-scale management and service-scale management are complementary, not superficially contradictory. The benign interaction between the two has a consistent improvement effect on green productivity in both the short and long term, which has spatial spillovers appearing in the long term. The reasons are as follows: The farmland rental market can reverse the inhibitory effect of the current low-maturity outsourcing services market on green productivity. The outsourcing services market can delay the arrival of the inflection point beyond which expansion of farmland rental transactions reduces green productivity, and amplify the positive effect of farmland rental on it. Although the degree of benign interaction between the two forms of large-scale management has gradually increased in recent years, it is still low overall. Agricultural large-scale management in China is still in the stage driven by land-scale management. Smallholder countries such as China need not worry prematurely about which large-scale management path to take, and they should treat both forms of large-scale management with an equal perspective to accelerate the high-level interaction between them.
Coupling Coordination Analysis of County Tourism Development and Multidimensional Poverty Based on Nighttime Light Data
Authors: Xiao Hai , Yu Jiahao , Zhang Yifan , Xin Chuliang , Wan Jiangjun , Tang Xiaohong
Published: May 2024

In China, tourism development is a crucial approach to poverty alleviation. With the consolidation of poverty alleviation achievements and the promotion of rural revitalization, it is of great significance to explore the relationship between tourism development and poverty alleviation from the perspective of multidimensional poverty. Therefore, this study took 28 key assistance counties for rural revitalization in the Sichuan–Chongqing region (hereinafter referred to as “key counties”) as the research objects, introduced NPP-VIIRS nighttime light (NTL) data, and a coupling coordination degree (CCD) model to explore the coordination relationship and mechanism between them. The results showed that from 2015 to 2020, the tourism development index (TDI) and estimated comprehensive development index (ECDI) of the key counties increased by 112.57% and 115.12%, respectively. In addition, the spatial differences in tourism development and multidimensional poverty both showed a narrowing trend. According to the results of the CCD model, the key counties basically faced coordination obstacles in the early stage, which were mainly transformed into reluctant coordination and moderate coordination in the later stage. This indicated that tourism poverty alleviation showed a coordinated development trend overall. However, the study also found that there may not be synchronicity between tourism development and poverty alleviation and analyzed the mechanism of their interaction. Overall, the study confirmed the positive impact of tourism development on alleviating multidimensional poverty. In addition, the study found that measuring multidimensional poverty based on NTL data has a high accuracy and can provide support for poverty research. These research results have an important reference value for China to carry out sustainable tourism poverty alleviation and comprehensively promote rural revitalization.
Coupling of Changing Trends in Population and Construction Land in Traditional Rural Areas and Spatial Patterns in Urban–Rural Development, 2016–2021: A Case Study of Heilongjiang Province, China
Authors: Lin Jia , Du Guoming , Zhang Ying , Yu Xiaoyang
Published: May 2024

Achieving the goal of integrated urban–rural development is to achieve a spatially balanced development of the constituent elements of urban–rural relations in China. Rural populations and land dedicated to construction are the main components of the countryside in traditional agricultural areas; they play an important role in the development of the countryside itself in terms of urban and rural land use and in the formation of urban and rural development patterns. This study analyzes the spatial and temporal changes in rural populations and construction land at the township level, alongside assessing various forms and the extent of coupled development. Herein, we consider the role of urban–rural attractiveness and propose a framework for relationships between urban and rural development based on different forms of coupled development; a model of urban–rural forces is constructed to determine spatial patterns of urban–rural development at the township level that may transpire in the future. Our study shows that the rural population and construction land in the study area are characterized by significant spatial and temporal dynamics, indicating that traditional rural areas are in a process of rapid development and change. The results of our measurements of township-level coupling indicate that there exist four development patterns within urban–rural development: the A-type is most likely to produce new cities or satellite towns in the future and form new urban areas; the B-type is the area most likely to cease and be annexed to other villages or cities to meet building targets; the C-type comprises areas to be focused on in the future to attract populations and strictly control the growth of rural construction land areas (to avoid land transforming into the B-type); and the D-type refers to lands upon which regional township centers may develop in the future, becoming an area devoted to rural revitalization. The A-type and D-type are prioritized for the allocation of construction land, which can be contracted from types B and C. The results of this study have provided important reference for the formulation of population and construction land control policies in accordance with local conditions and the realization of integrated urban and rural development strategies.
Risk of Atrial Fibrillation in Patients with Different Cancer Types in Taiwan
Authors: Liao Kuang-Ming , Yu Chia-Hung , Wu Yu-Cih , Wang Jhi-Joung , Liang Fu-Wen , Ho Chung-Han
Published: May 2024

Atrial fibrillation (AF) commonly occurs in approximately 2% of cancer patients, and the incidence of AF among cancer patients is greater than in the general population. This observational study presented the incidence risk of AF among cancer patients, including specific cancer types, using a population database. The Taiwan Cancer Registry was used to identify cancer patients between 2008 and 2017. The diagnosis of AF was based on the International Classification of Diseases codes (ICD-9-CM: 427.31 or ICD-10-CM: I48.0, I48.1, I48.2, and I48.91) in Taiwan national health insurance research datasets. The incidence of developing AF in the cancer population was calculated as the number of new-onset AF cases per person-year of follow-up during the study period. The overall incidence of AF among cancer patients was 50.99 per 100,000 person-years. Patients aged older than 65 years and males had higher AF incidence rates. Lung cancer males and esophageal cancer females showed the highest AF incidence risk (185.02 and 150.30 per 100,000 person-years, respectively). Our findings identified esophageal, lung, and gallbladder cancers as the top three cancers associated with a higher incidence of AF. Careful monitoring and management of patients with these cancers are crucial for early detection and intervention of AF.
Oxidative Stress, Persistent Inflammation and Blood Coagulation Alterations in Serum Proteome of Patients with Neovascular Age-Related Macular Degeneration
Authors: Winiarczyk Mateusz , Thiede Bernd , Utheim Tor Paaske , Kaarniranta Kai , Winiarczyk Dagmara , Michalak Katarzyna , Mackiewicz Jerzy
Published: May 2024

Neovascular age-related macular degeneration (AMD) is a major cause of irreversible blindness in elderly populations in developed countries. AMD’s etiopathology is multifactorial, with strong environmental and genetic components, but the exact molecular pathomechanisms underlying the disease are still unknown. In this study, we analyzed blood serum collected from 74 neovascular AMD patients and 58 healthy controls to identify proteins that may serve as potential biomarkers and expand our knowledge about the etiopathogenesis of the disease. The study revealed 17 differentially expressed proteins—11 up-regulated and 6 down-regulated—in neovascular AMD, which are involved in the biological processes previously linked with the disease—oxidative stress and persistent inflammation, impaired cellular transport, lipid metabolism and blood coagulation. In conclusion, the differences in the expressions of the proteins identified in this study may contribute to our understanding of the mechanisms underlying AMD and possibly serve in future as promising biomarkers.
Prenatal Diagnosis of Fryns Syndrome through Identification of Two Novel Splice Variants in the PIGN Gene—A Case Series
Authors: Marchetto Aruna , Leidescher Susanne , van Hoi Theresia , Hirschberger Niklas , Vogel Florian , Köhler Siegmund , Bedei Ivonne Alexandra , Axt-Fliedner Roland , Shoukier Moneef , Keil Corinna
Published: May 2024

Fryns syndrome (FS) is a multiple congenital anomaly syndrome with different multisystemic malformations. These include congenital diaphragmatic hernia, pulmonary hypoplasia, and craniofacial dysmorphic features in combination with malformations of the central nervous system such as agenesis of the corpus callosum, cerebellar hypoplasia, and enlarged ventricles. We present a non-consanguineous northern European family with two recurrent cases of FS: a boy with multiple congenital malformations who died at the age of 2.5 months and a female fetus with a complex developmental disorder with similar features in a following pregnancy. Quad whole exome analysis revealed two likely splicing-affecting disease-causing mutations in the PIGN gene: a synonymous mutation c.2619G>A, p.(Leu873=) in the last nucleotide of exon 29 and a 30 bp-deletion c.996_1023+2del (NM_176787.5) protruding into intron 12, with both mutations in trans configuration in the affected patients. Exon skipping resulting from these two variants was confirmed via RNA sequencing. Our molecular and clinical findings identified compound heterozygosity for two novel splice-affecting variants as the underlying pathomechanism for the development of FS in two patients.
Systematic Development of a Novel Laser-Sintering Machine with Roving Integration and Sustainability Evaluation
Authors: Baranowski Michael , Scholz Johannes , Kößler Florian , Fleischer Jürgen
Published: May 2024

Incorporating continuous carbon fibre-reinforced polymer (CCFRP) parts within additive manufacturing processes presents a significant advancement in the fabrication of robust lightweight parts, particularly relevant to aerospace, engineering, and various industrial sectors. Nonetheless, prevailing additive manufacturing methodologies for CCFRP parts exhibit notable limitations. Techniques reliant on resin and extrusion entail extensive and costly post-processing procedures to eliminate support structures, constraining design versatility and complicating small-scale production endeavours. In contrast, laser sintering (LS) emerges as a promising avenue for industrial application. It facilitates the efficient and cost-effective manufacturing of resilient parts without needing support structures. However, the current state of research and technological capabilities has yet to yield an LS machine that integrates the benefits of continuous fibre reinforcement with the inherent advantages of the LS process. This paper describes the systematic development process according to VDI 2221 of a new type of LS machine with automated continuous fibre integration while keeping the advantages of the LS process. The resulting physical prototype of the machine is also presented. Furthermore, this study presents an approach to integrate the cost and Product Carbon Footprint of the process in the product design. For this purpose, a machine state model was developed, and the costs and Product Carbon footprint of a part were analysed based on the model. The promising potential for future lightweight products is demonstrated through the production of CCFRP parts.
Role of Type 4B Secretion System Protein, IcmE, in the Pathogenesis of Coxiella burnetii
Authors: Palanisamy Rajesh , Zhang Yan , Zhang Guoquan
Published: May 2024

Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes Q fever, a life-threatening zoonotic disease. C. burnetii replicates within an acidified parasitophorous vacuole derived from the host lysosome. The ability of C. burnetii to replicate and achieve successful intracellular life in the cell cytosol is vastly dependent on the Dot/Icm type 4B secretion system (T4SSB). Although several T4SSB effector proteins have been shown to be important for C. burnetii virulence and intracellular replication, the role of the icmE protein in the host–C. burnetii interaction has not been investigated. In this study, we generated a C. burnetii Nine Mile Phase II (NMII) mutant library and identified 146 transposon mutants with a single transposon insertion. Transposon mutagenesis screening revealed that disruption of icmE gene resulted in the attenuation of C. burnetii NMII virulence in SCID mice. ELISA analysis indicated that the levels of pro-inflammatory cytokines, including interleukin-1β, IFN-γ, TNF-α, and IL-12p70, in serum from Tn::icmE mutant-infected SCID mice were significantly lower than those in serum from wild-type (WT) NMII-infected mice. Additionally, Tn::icmE mutant bacteria were unable to replicate in mouse bone marrow-derived macrophages (MBMDM) and human macrophage-like cells (THP-1). Immunoblotting results showed that the Tn::icmE mutant failed to activate inflammasome components such as IL-1β, caspase 1, and gasdermin-D in THP-1 macrophages. Collectively, these results suggest that the icmE protein may play a vital role in C. burnetii virulence, intracellular replication, and activation of inflammasome mediators during NMII infection.
Susceptibility and Resilience, a Fig Tree and a Scream
Authors: Saunders Rebecca
Published: May 2024

Analyzing two key figures in Elif Shafak’s novel The Island of Missing Trees—a schoolgirl’s scream and a narrating fig tree—this essay analyzes the intersection between susceptibility and resilience, particularly as these terms are developed in psychology, trauma studies, and ecology. I argue that the novel’s resonant scream critiques the discourse of psychological resilience on multiple counts: its inadequacy as a response to complex trauma, its focus on autonomous individuals, its assumption that responsibility for resilience rests on victims rather than perpetrators of harm, its construction of a “resistance imperative” and its disavowal of the inequalities in access to resilience-building resources. By contrast, the novel’s fig tree, I contend, exemplifies an ecological model of resilience rooted in a recognition of the interdependence of the multiple and diverse organisms that comprise an ecosystem, and of susceptibility as an advantageous suite of capacities that are crucial to resilience. These contrasting conceptions of resilience lead me to advocate for a politics of susceptibility, an eco-psychosocial politics based on the recognition that individuals cannot become resilient on their own, through their own volition, intention, or “self-efficacy”, and that focuses instead on building systemic and sustainable forms of resilience inclusive of the diverse subjects that comprise a community, society or ecosystem; that, rather than fetishizing independence, liberty and rights, fortifies interdependence and reinforces mutual responsibilities; and that rather than exploiting susceptibility as a weakness, nurtures it as the soul of resilience itself.
Crystal ZnGeP2 for Nonlinear Frequency Conversion: Physical Parameters, Phase-Matching and Nonlinear Properties: Revision
Authors: Grechin Sergey G. , Muravev Ilyia A.
Published: May 2024

The article presents a comparative analysis of published data for the physical parameters of the ZGP (ZnGeP2) crystal, its nonlinear and phase-matching properties, and functional capabilities for all frequency conversion processes (harmonics, sum and difference frequencies, and parametric generation). At the first time, the possibilities for obtaining the temperature-noncritical processes for some combinations of wavelengths are shown.
CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)
Authors: Huang Yanzhong , Wu Juanzi , Lin Jianyu , Liu Zhiwei , Mao Zhengfeng , Qian Chen , Zhong Xiaoxian
Published: May 2024

The degree of forage lignification is a key factor affecting its digestibility by ruminants such as cattle and sheep. Sudan grass (Sorghum sudanense S.) is a high-quality sorghum forage, and its lignocellulose is mostly stored in the secondary cell wall. However, the secondary cell wall synthesis mechanism of Sudan grass has not yet been studied in depth. To further study the secondary cell wall synthesis mechanism of Sudan grass using established transcriptome data, this study found that CcNAC6, a homologous gene of Arabidopsis AtSND2, is related to the secondary cell wall synthesis of Sudan grass. Accordingly, we constructed a CcNAC6-overexpressing line of Arabidopsis to investigate the function of the CcNAC6 gene in secondary cell wall synthesis. The results showed that the overexpression of the CcNAC6 gene could significantly increase the lignin content of Arabidopsis. Based on subcellular localization analysis, CcNAC6 is found in the nucleus. In addition, yeast two-hybridization screening showed that CcCP1, associated with secondary cell wall synthesis, can interact with CcNAC6. Therefore, the above results indicate that CcNAC6 has a positive regulatory effect on the secondary cell wall synthesis of Sudan grass, and it is speculated that CcNAC6 may be the main regulator of the secondary cell wall synthesis of Sudan grass through its interaction with another regulatory protein, CcCP1. This study provides a theoretical basis and new genetic resources for the creation of new Sudan grass germplasm with a low lignin content.
Genome-Wide Identification and Characterization of the GRAS Gene Family in Lettuce Revealed That Silencing LsGRAS13 Delayed Bolting
Authors: Chen Li , Qin Yong , Fan Shuangxi
Published: May 2024

Lettuce is susceptible to high-temperature stress during cultivation, leading to bolting and affecting yield. Plant-specific transcription factors, known as GRAS proteins, play a crucial role in regulating plant growth, development, and abiotic stress responses. In this study, the entire lettuce LsGRAS gene family was identified. The results show that 59 LsGRAS genes are unevenly distributed across the nine chromosomes. Additionally, all LsGRAS proteins showed 100% nuclear localization based on the predicted subcellular localization and were phylogenetically classified into nine conserved subfamilies. To investigate the expression profiles of these genes in lettuce, we analyzed the transcription levels of all 59 LsGRAS genes in the publicly available RNA-seq data under the high-temperature treatment conducted in the presence of exogenous melatonin. The findings indicate that the transcript levels of the LsGRAS13 gene were higher on days 6, 9, 15, 18, and 27 under the high-temperature (35/30 °C) treatment with melatonin than on the same treatment days without melatonin. The functional studies demonstrate that silencing LsGRAS13 accelerated bolting in lettuce. Furthermore, the paraffin sectioning results showed that flower bud differentiation in LsGRAS13-silenced plants occurred significantly faster than in control plants. In this study, the LsGRAS genes were annotated and analyzed, and the expression pattern of the LsGRAS gene following melatonin treatment under high-temperature conditions was explored. This exploration provides valuable information and identifies candidate genes associated with the response mechanism of lettuce plants high-temperature stress.
Cladodes of Opuntia ficus-indica (L.) Mill. Possess Important Beneficial Properties Dependent on Their Different Stages of Maturity
Authors: Maiuolo Jessica , Nucera Saverio , Serra Maria , Caminiti Rosamaria , Oppedisano Francesca , Macrì Roberta , Scarano Federica , Ragusa Salvatore , Muscoli Carolina , Palma Ernesto , Mollace Vincenzo
Published: May 2024

Opuntia ficus-indica (L.) Mill. belongs to the Cactaceae family and the genus Opuntia; it is a succulent plant that adapts to extreme climatic conditions. The aerial part of the plant consists of the cladodes, morphological changes of branches that appear green, are covered with thorns, and are essential to reduce excessive perspiration of water. The composition of cladodes is very varied, and the main constituents are water, fibers, polysaccharides, proteins, fatty acids, vitamins, sterols, minerals, and polyphenols. Polyphenols are responsible for many beneficial activities for human health, such as antioxidant, anti-inflammatory, anticancer, and nutritional properties. The purpose of this manuscript was to compare the properties of cladodes belonging to the same plant but with different stages of maturity. Relative extracts were tested both in vitro and on a cell line and antioxidant and anti-apoptotic properties were found. The antioxidant activity was tested by the Oxygen Radical Absorbance Capacity (ORAC) test, the 1,1-diphenyl-2-picrylhydrazil (DPPH) test, and the measurement of cellular accumulation of reactive oxygen species (ROS). Anti-apoptotic activity was evaluated by the annexin/PI assay and measurement of caspases 9 and 3 expression. The results obtained showed that the extracts considered possess antioxidant and anti-apoptotic properties. However, the different stages of maturity of cladodes are essential for the performance of both functions. In addition, important variations were made in the dissolution of the extracts that brought greater safety in their use. In conclusion, this manuscript provides further information on cladodes of Opuntia ficus-indica, which can be used as adjuvants in many human pathologies.