bluuebunny/crossref_metadata_2025 huggingface dataset:
Utility of a very high IRT/No mutation referral category in cystic fibrosis newborn screening
Authors: Kay Denise M. , Langfelder‐Schwind Elinor , DeCelie‐Germana Joan , Sharp Jack K. , Maloney Breanne , Tavakoli Norma P. , Saavedra‐Matiz Carlos A. , Krein Lea M. , Caggana Michele , Kier Catherine ,   the New York State Cystic Fibrosis Newborn Screening Consortium
Published: June 2015

Newborn screening for Cystic Fibrosis (CF) began in New York in October, 2002 using immunoreactive trypsinogen (IRT)/DNA methodology. Infants with at least one CFTR mutation or very high IRT and no mutations (VHIRT) are referred for sweat testing. In a preliminary analysis, we noted a very low positive predictive value (PPV) and preponderance of Hispanic infants in the group of infants with CF referred for VHIRT, which led to a decision to revise, but not eliminate, the VHIRT category. Automatic referral for specimens with VHIRT collected on the day of birth was eliminated, and the VHIRT threshold was raised from 0.2% to 0.1%. In this report, we describe outcomes from VHIRT referrals among 2.4 million infants screened between March 2003 and February 2013. Following the algorithm change, referrals decreased by 37.8% overall (annual mean 1,485 vs. 923), and the VHIRT PPV improved (0.6–1.0%). The number of infants diagnosed has remained consistent at 1 in 4,400 births. The proportion of Black/Hispanic/Asian/Other infants with confirmed CF, CFTR‐related metabolic syndrome (CRMS), or possible CF/CRMS was 21.3% in infants with 1–2 mutations, but 75.8% in the VHIRT group. In conclusion, although the PPV among VHIRT referrals remains low, had this category never been implemented, 24 infants with confirmed CF, and 9 infants with CRMS or possible CF/CRMS, most of whom were Hispanic, would have been missed over the 10 years. Information from this study may be helpful in assessing the need for the VHIRT category and algorithm changes in other screening programs. Pediatr Pulmonol. 2015; 50:771–780. © 2015 Wiley Periodicals, Inc.
Sublethal doses of fipronil intensify synapsin immunostaining in Atta sexdens rubropilosa (Hymenoptera: Formicidae) brains
Authors: Cintra‐Socolowski Priscila , Roat Thaisa C , Nocelli Roberta CF , Nunes Pablo H , Ferreira Rafael AC , Malaspina Osmar , Bueno Odair C
Published: June 2015

BACKGROUND

Although ants are common insects in agricultural ecosystems, few studies have considered how xenobiotics might induce physiological and morphological alterations in these insects. This study aimed to verify the neurotoxic action of sublethal doses of fipronil on the mushroom bodies of brains from the leaf‐cutting ant Atta sexdens rubropilosa through immunocytochemistry analysis for the protein synapsin.

RESULTS

The LD50 value was established as 1.42 ng ant−1, and the sublethal doses used were LD50/10 and LD50/100. Synapsin labelling was more evident in the brains extracted from ants exposed to the insecticide, specifically in the regions of glia in the mushroom bodies, compared with the control group. It was possible to measure the intensity of emitted fluorescence in the areas of the mushroom bodies, and a statistical test showed differences between the control group and the treatment group.

CONCLUSION

Thus, it is concluded that sublethal doses of the insecticide fipronil intensified synapsin immunostaining, suggesting an increased release of neurotransmitters, which may be linked to neurotoxicity and overexcitation. These sublethal doses may have two different effects: compromising the operation and maintenance of the colony and leading to the establishment of resistance in insects. © 2015 Society of Chemical Industry
Measures of agreement between many raters for ordinal classifications
Authors: Nelson Kerrie P. , Edwards Don
Published: June 2015

Screening and diagnostic procedures often require a physician's subjective interpretation of a patient's test result using an ordered categorical scale to define the patient's disease severity. Because of wide variability observed between physicians' ratings, many large‐scale studies have been conducted to quantify agreement between multiple experts' ordinal classifications in common diagnostic procedures such as mammography. However, very few statistical approaches are available to assess agreement in these large‐scale settings. Many existing summary measures of agreement rely on extensions of Cohen's kappa. These are prone to prevalence and marginal distribution issues, become increasingly complex for more than three experts, or are not easily implemented. Here we propose a model‐based approach to assess agreement in large‐scale studies based upon a framework of ordinal generalized linear mixed models. A summary measure of agreement is proposed for multiple experts assessing the same sample of patients' test results according to an ordered categorical scale. This measure avoids some of the key flaws associated with Cohen's kappa and its extensions. Simulation studies are conducted to demonstrate the validity of the approach with comparison with commonly used agreement measures. The proposed methods are easily implemented using the software package R and are applied to two large‐scale cancer agreement studies. Copyright © 2015 John Wiley & Sons, Ltd.
Meta‐analysis of time‐to‐event outcomes from randomized trials using restricted mean survival time: application to individual participant data
Authors: Wei Yinghui , Royston Patrick , Tierney Jayne F. , Parmar Mahesh K. B.
Published: June 2015

Meta‐analysis of time‐to‐event outcomes using the hazard ratio as a treatment effect measure has an underlying assumption that hazards are proportional. The between‐arm difference in the restricted mean survival time is a measure that avoids this assumption and allows the treatment effect to vary with time. We describe and evaluate meta‐analysis based on the restricted mean survival time for dealing with non‐proportional hazards and present a diagnostic method for the overall proportional hazards assumption. The methods are illustrated with the application to two individual participant meta‐analyses in cancer. The examples were chosen because they differ in disease severity and the patterns of follow‐up, in order to understand the potential impacts on the hazards and the overall effect estimates. We further investigate the estimation methods for restricted mean survival time by a simulation study. Copyright © 2015 John Wiley & Sons, Ltd.
Optimization of multi‐stage dynamic treatment regimes utilizing accumulated data
Authors: Huang Xuelin , Choi Sangbum , Wang Lu , Thall Peter F.
Published: June 2015

In medical therapies involving multiple stages, a physician's choice of a subject's treatment at each stage depends on the subject's history of previous treatments and outcomes. The sequence of decisions is known as a dynamic treatment regime or treatment policy. We consider dynamic treatment regimes in settings where each subject's final outcome can be defined as the sum of longitudinally observed values, each corresponding to a stage of the regime. Q‐learning, which is a backward induction method, is used to first optimize the last stage treatment then sequentially optimize each previous stage treatment until the first stage treatment is optimized. During this process, model‐based expectations of outcomes of late stages are used in the optimization of earlier stages. When the outcome models are misspecified, bias can accumulate from stage to stage and become severe, especially when the number of treatment stages is large. We demonstrate that a modification of standard Q‐learning can help reduce the accumulated bias. We provide a computational algorithm, estimators, and closed‐form variance formulas. Simulation studies show that the modified Q‐learning method has a higher probability of identifying the optimal treatment regime even in settings with misspecified models for outcomes. It is applied to identify optimal treatment regimes in a study for advanced prostate cancer and to estimate and compare the final mean rewards of all the possible discrete two‐stage treatment sequences. Copyright © 2015 John Wiley & Sons, Ltd.
Multiple imputation for harmonizing longitudinal non‐commensurate measures in individual participant data meta‐analysis
Authors: Siddique Juned , Reiter Jerome P. , Brincks Ahnalee , Gibbons Robert D. , Crespi Catherine M. , Brown C. Hendricks
Published: June 2015

There are many advantages to individual participant data meta‐analysis for combining data from multiple studies. These advantages include greater power to detect effects, increased sample heterogeneity, and the ability to perform more sophisticated analyses than meta‐analyses that rely on published results. However, a fundamental challenge is that it is unlikely that variables of interest are measured the same way in all of the studies to be combined. We propose that this situation can be viewed as a missing data problem in which some outcomes are entirely missing within some trials and use multiple imputation to fill in missing measurements. We apply our method to five longitudinal adolescent depression trials where four studies used one depression measure and the fifth study used a different depression measure. None of the five studies contained both depression measures. We describe a multiple imputation approach for filling in missing depression measures that makes use of external calibration studies in which both depression measures were used. We discuss some practical issues in developing the imputation model including taking into account treatment group and study. We present diagnostics for checking the fit of the imputation model and investigate whether external information is appropriately incorporated into the imputed values. Copyright © 2015 John Wiley & Sons, Ltd.
Learning the Confucian Way
Authors: Guo Tieyuan
Published: June 2015

In this chapter, the focus is on understanding the unique learning motivations shared by contemporary students in Confucian heritage cultures.
Authentic Assessment of Knowledge, Skills, and Attitudes
Authors: Litchfield Brenda C. , Dempsey John V.
Published: June 2015

This chapter proposes supplementing traditional assessment strategies with innovative and authentic ways to evaluate students’ performance on intended learning outcomes.
Transcriptional responses in male Japanese medaka exposed to antiandrogens and antiandrogen/androgen mixtures
Authors: Sun Liwei , Peng Tao , Liu Fang , Ren Lin , Peng Zuhua , Ji Guorong , Zhou Yinfang , Fu Zhengwei
Published: June 2015

The occurrence of androgenic endocrine disrupting chemicals (EDCs) in water is thought to be linked to deviation from normal male developmental and reproductive functions in exposed aquatic organisms. Because aquatic environments represent a chemically complex medium, the combined effects of androgenic EDCs require urgent attention. In the present study, the effects of two model androgen receptor (AR) antagonists, flutamide (FLU), and vinclozolin (VIN), were first determined individually in male Japanese medaka using the transcriptional response for genes associated with the hypothalamic‐pituitary‐gonadal axis. The fish were further exposed to binary mixtures of VIN and 17β‐trenbolone (TRE, AR agonist) to confirm the theoretical opposing effects of the AR antagonist and agonist. The results showed that exposure to FLU or VIN alone induced very similar transcriptional responses, demonstrating that gene transcription analysis could be successfully employed in identifying the action of single chemicals. For example, both exposures increased the transcription of cyp17b but decreased that of cyp19b in the gonad, demonstrating the compensatory response for AR blockage. However, in the case of exposure to mixtures, although the joint antagonistic action of TRE and VIN affected the most genes, the transcription profiles after exposure to mixtures were not consistent with expectations based on the results for individual chemicals, such as hepatic vtg, and star or cyp19a in gonads. Therefore, the limitation of gene transcription analyses in exposures to mixtures, as well as the potential for the extrapolation of single chemicals, should be considered in future studies. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1591–1599, 2016.
Social learning and climate change adaptation: evidence for international development practice
Authors: Ensor Jonathan , Harvey Blane
Published: June 2015

The potential for social learning to address complex, interconnected social and environmental challenges, such as climate change adaptation, is receiving increasing attention in research and practice. Social learning approaches vary, but commonly include cycles of knowledge sharing and joint action to co‐create knowledge, relationships, and practices among diverse stakeholders. This results in learning and change that goes beyond the individual into communities, networks, or systems. Many authors have focused on analysis of case studies to better understand the contexts in which such learning occurs. In this paper, we look across this literature to draw out lessons for international development practice. To support those looking to purposively design social learning interventions for adaptation, we focus on four areas: lessons learned and the principles adopted when using a social learning approach, examples of tools and methods used, approaches to evaluating social learning, and examples of its impact. While we identify important lessons for practice within each of these areas, three cross‐cutting themes emerge. These are: the importance of developing a shared view among those initiating learning processes of how change might happen and of how social learning fits within it, linking this locus of desired change to the tools employed; the centrality of skilled facilitation and in particular how practitioners may shift toward being participants in the collective learning process; and the need to attend to social difference, recognizing the complexity of social relations and the potential for less powerful actors to be co‐opted in shared decision making.WIREs Clim Change2015, 6:509–522. doi: 10.1002/wcc.348

This article is categorized under:

Vulnerability and Adaptation to Climate Change > Learning from Cases and Analogies

Climate and Development > Knowledge and Action in Development
Nanoparticle‐motivated gene delivery for ophthalmic application
Authors: Mitra Rajendra Narayan , Zheng Min , Han Zongchao
Published: June 2015

Ophthalmic gene therapy is an intellectual and intentional manipulation of desired gene expression into the specific cells of an eye for the treatment of ophthalmic (ocular) genetic dystrophies and pathological conditions. Exogenous nucleic acids such as DNA, small interfering RNA, micro RNA, and so on, are used for the purpose of managing expression of the desired therapeutic proteins in ocular tissues. The delivery of unprotected nucleic acids into the cells is limited because of exogenous and endogenous degradation modalities. Nanotechnology, a promising and sophisticated cutting edge tool, works as a protective shelter for these therapeutic nucleic acids. They can be safely delivered to the required cells in order to modulate anticipated protein expression. To this end, nanotechnology is seen as a potential and promising strategy in the field of ocular gene delivery. This review focused on current nanotechnology modalities and other promising nonviral strategies being used to deliver therapeutic genes in order to treat various devastating ocular diseases. WIREs Nanomed Nanobiotechnol 2015, 8:160–174. doi: 10.1002/wnan.1356

This article is categorized under:

Nanotechnology Approaches to Biology > Nanoscale Systems in Biology
Influence of the Oil Phase on the Microencapsulation by Complex Coacervation
Authors: Prata Ana S. , Grosso Carlos R.F.
Published: June 2015

The influence of oil type on the process yield, efficiency of encapsulation, particle size and morphological aspects of coacervated microparticles was investigated. Firstly, several factors affecting microencapsulation of oils by complex coacervation were simultaneously examined. The results indicated that the process yield is mainly dependent on the velocity of homogenization, temperature and polymer ratio. Using optimum conditions for producing microparticles [pH 4.0, 14,000 rpm, 50 °C, gelatin:gum arabic (GE:GA) 1:1 and 2.5 % w/v], different core materials were tested: a vegetable oil (almond oil), an oil with higher hydrophilic lipophilic balance (vetiver essential oil) and a highly hydrophobic oil (mineral oil). The oil phase exerted an influence on microparticle formation, disturbing the complexation of polymers and modifying the core distribution within the particles. Some of the polymers were complexed when mineral oil was used, and the highest efficiency of encapsulation (91.8 %) was obtained with vetiver oil, followed by the almond (70.6 %) and mineral (38.0 %) oils. Particles produced with vetiver oil were larger (43.5 μm) than those produced with mineral oil (35.0 μm) and almond oil (19.2 μm), and the increase in the size is due to the encapsulation of many small droplets of emulsion, characterizing these particles as multinucleate ones.
Surfactant Concentration, Antioxidants, and Chelators Influencing Oxidative Stability of Water‐in‐Walnut Oil Emulsions
Authors: Yi Jianhua , Dong Wenbin , Zhu Zhenbao , Liu Ning , Ding Yong , McClements David Julian , Decker Eric Andrew
Published: June 2015

Effects of surfactant concentration, antioxidants with different polarities, and chelator type on the oxidative stability of water‐in‐stripped walnut oil (W/O) emulsions stabilized by polyglycerol polyricinoleate (PGPR) were evaluated. The formation of primary oxidation products (lipid hydroperoxides) and secondary oxidation products (hexanal) decreased with increasing PGPR concentrations (0.3–1.0 wt% of emulsions). Excess surfactant might solubilize lipid hydroperoxides out of the oil–water interface, resulting in the decreased lipid oxidation rates in W/O emulsions. At concentrations of 10–1000 μM, the polar Trolox demonstrated concentration‐dependent antioxidant activity according to both hydroperoxide and hexanal formation. The antioxidant efficiency of the non‐polar α‐tocopherol was slightly reduced at the higher range of 500–1000 μM based on hydroperoxide formation. Both ethylenediaminetetraacetic acid (EDTA) and deferoxamine (DFO) at concentrations of 5–100 μM reduced the rates of lipid oxidation at varying degrees, indicating that endogenous transition metals may promote lipid oxidation in W/O emulsions. EDTA was a stronger inhibitor of lipid oxidation than DFO. These results suggest that the oxidative stability of W/O emulsions could be improved by the appropriate choice of surfactant concentration, antioxidants, and chelators.
For Better or Worse: The Health Implications of Marriage Separation Due to Migration in Rural China
Authors: Chen Feinian , Liu Hui , Vikram Kriti , Guo Yu
Published: June 2015

Massive rural-to-urban migration in China has led to spatial separation of millions of married couples. In this article, we examine the question of whether the well-documented health benefits of marriage extend to left-behind individuals in rural China who are spatially separated from their spouses. Using longitudinal data that span 16 years (China Health and Nutrition Survey 1991, 1993, 1997, 2000, 2004, 2006), we compare the self-reported health trajectories of adults across different marital statuses while taking into account the physical location of their spouses. Our results suggest a clear health disadvantage of married individuals whose spouses are absent compared with those whose spouses are living in the same household. Further, longer spousal absence is more harmful to an individual’s health. Finally, spousal absence and longer physical separation from their spouses induce stronger health deficits for married men than for married women, suggesting that a gendered process is at work.
Gender, Power, and Emigration From Mexico
Authors: Nobles Jenna , McKelvey Christopher
Published: June 2015

The prevailing model of migration in developing countries conceives of a risk-diversifying household in which members act as a single entity when making migration decisions. Ethnographic studies challenge this model by documenting gender hierarchy in family decisions and arguing that, in many contexts, men and women have differing views on the value of migration. We assess these perspectives using longitudinal survey data from Mexico. We show that Mexican households are heterogeneous in terms of women’s decision-making authority and control over resources, and this variation predicts the subsequent emigration of their male partners to the United States. We then use data from a policy experiment to demonstrate that an exogenous increase in a woman’s control over household resources decreases the probability that her spouse migrates. Our findings support the presence of important gender differences in how migration is valued. They also suggest that women’s role in these decisions is inadvertently underrepresented in studies of migrant families. Staying is also a migration decision, and it is more likely in homes in which women have greater authority. From a policy perspective, the results suggest that Mexican migration is influenced not only by increases in household resources but also by which members of the household control them.
Daily life with depressive symptoms: Gender differences in adolescents' everyday emotional experiences
Authors: Frost Allison , Hoyt Lindsay T. , Chung Alissa Levy , Adam Emma K.
Published: June 2015

Depression is a prevalent and debilitating illness facing many adolescents, especially adolescent girls, whose risk for this disorder is approximately twice that of boys. Many studies have identified mechanisms that place girls at higher risk for depression during adolescence. Few, however, have examined differences in the everyday emotional experiences of boys and girls with varying levels of depressive symptoms. Using the Experience Sampling Method, this study investigated the roles of gender and depressive symptomatology in the emotional experiences of a community sample of youth (11–18 year‐olds) from the Sloan 500 Family Study. Females with higher levels of depressive symptoms were more likely than females with fewer depressive symptoms and all males to experience strong negative emotions and to attribute the cause of these emotions to other people. These results suggest that emotional reactivity in interpersonal contexts is especially important to understand gender differences in the daily experience of depressive symptoms.
Is the ferret a suitable species for studying perinatal brain injury?
Authors: Empie Kristen , Rangarajan Vijayeta , Juul Sandra E.
Published: June 2015

Complications of prematurity often disrupt normal brain development and/or cause direct damage to the developing brain, resulting in poor neurodevelopmental outcomes. Physiologically relevant animal models of perinatal brain injury can advance our understanding of these influences and thereby provide opportunities to develop therapies and improve long‐term outcomes. While there are advantages to currently available small animal models, there are also significant drawbacks that have limited translation of research findings to humans. Large animal models such as newborn pig, sheep and nonhuman primates have complex brain development more similar to humans, but these animals are expensive, and developmental testing of sheep and piglets is limited. Ferrets (Mustela putorius furo) are born lissencephalic and undergo postnatal cortical folding to form complex gyrencephalic brains. This review examines whether ferrets might provide a novel intermediate animal model of neonatal brain disease that has the benefit of a gyrified, altricial brain in a small animal. It summarizes attributes of ferret brain growth and development that make it an appealing animal in which to model perinatal brain injury. We postulate that because of their innate characteristics, ferrets have great potential in neonatal neurodevelopmental studies.
Preparing the next generation of maternal and newborn health leaders: The maternal and newborn health champions initiatives
Authors: Dao Blami , Otolorin Emmanuel , Gomez Patricia P. , Carr Catherine , Sanghvi Harshad
Published: June 2015

A champion in health care can be defined as any health professional who has the requisite knowledge and skills in a relevant health field, who is respected by his/her peers and supported by his/her supervisors, and who takes the lead to promote or introduce evidence‐based interventions to improve the quality of care. Jhpiego used a common approach during two distinct initiatives to identify individuals in Africa, Asia, and Latin America and the Caribbean whose expertise in their clinical service area and whose leadership capacity could be strengthened to enable them to serve as champions for maternal and newborn health (MNH). These champions have gone on to contribute to the improvement of MNH in their respective countries and regions. The lessons learned from this approach are shared so they can be used by other organizations to design leadership development strategies for MNH in low‐resource countries.
GLEAM: The GaLactic and Extragalactic All-Sky MWA Survey
Authors: Wayth R. B. , Lenc E. , Bell M. E. , Callingham J. R. , Dwarakanath K. S. , Franzen T. M. O. , For B.-Q. , Gaensler B. , Hancock P. , Hindson L. , Hurley-Walker N. , Jackson C. A. , Johnston-Hollitt M. , Kapińska A. D. , McKinley B. , Morgan J. , Offringa A. R. , Procopio P. , Staveley-Smith L. , Wu C. , Zheng Q. , Trott C. M. , Bernardi G. , Bowman J. D. , Briggs F. , Cappallo R. J. , Corey B. E. , Deshpande A. A. , Emrich D. , Goeke R. , Greenhill L. J. , Hazelton B. J. , Kaplan D. L. , Kasper J. C. , Kratzenberg E. , Lonsdale C. J. , Lynch M. J. , McWhirter S. R. , Mitchell D. A. , Morales M. F. , Morgan E. , Oberoi D. , Ord S. M. , Prabu T. , Rogers A. E. E. , Roshi A. , Shankar N. Udaya , Srivani K. S. , Subrahmanyan R. , Tingay S. J. , Waterson M. , Webster R. L. , Whitney A. R. , Williams A. , Williams C. L.
Published: June 2015

GLEAM, the GaLactic and Extragalactic All-sky MWA survey, is a survey of the entire radio sky south of declination + 25° at frequencies between 72 and 231 MHz, made with the MWA using a drift scan method that makes efficient use of the MWA’s very large field-of-view. We present the observation details, imaging strategies, and theoretical sensitivity for GLEAM. The survey ran for two years, the first year using 40-kHz frequency resolution and 0.5-s time resolution; the second year using 10-kHz frequency resolution and 2 s time resolution. The resulting image resolution and sensitivity depends on observing frequency, sky pointing, and image weighting scheme. At 154 MHz, the image resolution is approximately 2.5 × 2.2/cos (δ + 26.7°) arcmin with sensitivity to structures up to ~ 10° in angular size. We provide tables to calculate the expected thermal noise for GLEAM mosaics depending on pointing and frequency and discuss limitations to achieving theoretical noise in Stokes I images. We discuss challenges, and their solutions, that arise for GLEAM including ionospheric effects on source positions and linearly polarised emission, and the instrumental polarisation effects inherent to the MWA’s primary beam.
Morphological and fecundity traits of Culex mosquitoes caught in gravid traps in urban and rural Berkshire, UK
Authors: Townroe S. , Callaghan A.
Published: June 2015

Culex pipiens s.l. is one of the primary vectors of West Nile Virus in the USA and Continental Europe. The seasonal abundance and eco-behavioural characteristics of the typical form, Cx. pipiens pipiens, make it a key putative vector in Britain. Surveillance of Culex larvae and adults is essential to detect any changes to spatial and seasonal activity or morphological traits that may increase the risk of disease transmission. Here we report the use of the modified Reiter gravid box trap, which is commonly used in the USA but scarcely used in the UK, to assess its suitability as a tool for British female Culex mosquito surveillance. Trapping was carried out at 110 sites in urban and rural gardens in Berkshire in May, July and September 2013. We tested if reproductively active adult female Culex are more abundant in urban than rural gardens and if wing characteristic traits and egg raft size are influenced by location and seasonal variations. Gravid traps were highly selective for Culex mosquitoes, on average catching significantly more per trap in urban gardens (32.4 ± 6.2) than rural gardens (19.3 ± 4.0) and more in July than in May or September. The majority of females were caught alive in a good condition. Wing lengths were measured as an indicator of size. Females flying in September were significantly smaller than females in May or July. Further non-significant differences in morphology and fecundity between urban and rural populations were found that should be explored further across the seasons.
Siemens and the Construction of Hospitals in Latin America, 1949–1964
Authors: Donzé Pierre-Yves
Published: June 2015

This article focuses on the construction of turnkey hospitals in Latin America by the German electromedical equipment maker Siemens-Reiniger-Werke (SRW) in the 1950s and 1960s. After World War II, SRW was unable to return to the overseas markets it had lost during the war through only the export of goods. Hospital construction was thus a way to reenter these markets, offering local governments a new form of service and product: a German hospital. In order to achieve this strategy, SRW organized and directed an informal association called Deutsche Hospitalia, which gathered together some thirty German manufacturers involved in the process of building a fully equipped hospital. This article argues that SRW, by diffusing a standardized hospital model in Latin America, contributed to the globalization of medicine.
Spatial variability of helminth parasites and evidence for stock discrimination in the round sardinella,Sardinella aurita(Valenciennes, 1847), off the coast of Tunisia
Authors: Feki M. , Châari M. , Neifar L.
Published: June 2015

Three digeneans –Parahemiurus merus(Linton, 1910),Aphanurus stossichii(Monticelli, 1891) andLecithochiriumsp. – and one tetraphyllidean cestode larva were used as biological tags to discriminate the stock ofSardinella aurita(Valenciennes, 1847). In total, 579 fish were examined in five zones off the Tunisian coast, including Bizerte and Kelibia in the north, Mahdia in the east, Gabes and Zarzis in the south. Discriminant analyses used for the separation ofS. auritaallowed for the identification of two discrete stocks.Sardinella auritafrom Bizerte, Kelibia and Zarzis clumped together as a single stock.Parahemiurus merusandA. stossichiiwere the most important species in determining the location of sampled fish from these regions. Specimens from Mahdia and Gabes were grouped as one stock characterized by the presence ofLecithochiriumsp. and larvae of the Tetraphyllidea. These results were corroborated by comparing the parameters of prevalence and mean abundance of parasites among zones. The separation ofS. auritabetween localities after pooling specimens from Bizerte, Kelibia and Zarzis and separately pooling those from Mahdia and Gabes also allowed the identification of two discrete stocks, one in offshore waters from Bizerte, Kelibia and Zarzis characterized by the digeneansP. merusandA. stossichiiand one in inshore waters from Mahdia and Gabes characterized byLechithochiriumsp. and tetraphyllidean larvae.
In vitro activity of essential oils of free and nanostructured Melaleuca alternifolia and of terpinen-4-ol on eggs and larvae of Haemonchus contortus
Authors: Grando T.H. , de Sá M.F. , Baldissera M.D. , Oliveira C.B. , de Souza M.E. , Raffin R.P. , Santos R.C.V. , Domingues R. , Minho A.P. , Leal M.L.R. , Monteiro S.G.
Published: June 2015

Haemonchus contortus is one of the major gastrointestinal nematodes responsible for significant economic and production losses of sheep. Diseases caused by this species lack effective anthelmintic products, and the search for new compounds to replace synthetic anthelmintics has been extensive. The present investigation assesses the in vitro activity of the essential oil of melaleuca (Melaleuca alternifolia), both free (TTO) and nanostructured (nanoTTO), and terpinen-4-ol (terp-4-ol) on eggs and larvae of H. contortus. Tests of egg hatching (EHT) and inhibition of larval migration (LMIT) were used to assess the in vitro efficacy of TTO, nanoTTO and terp-4-ol. Using EHT, at a concentration of 3.5 mg/ml, 100% inhibition occurred using TTO and terp-4-ol, with LC50 values of 0.43 and 0.63 mg/ml, and LC90 values of 1.75 mg/ml and 3.12 mg/ml, respectively. NanoTTO had lower activity, with 82.6% inhibition at the same concentration. Using LMIT, TTO and nanoTTO had a similar activity with 88.0% and 84.8% inhibition, respectively, at a concentration of 56 mg/ml. Terp-4-ol had a greater effect on larvae, with 85.7% inhibition at a concentration of 56 mg/ml and 82.4% at 3.5 mg/ml, demonstrating high activity at the lowest concentration tested. Therefore, the results indicate that all substances tested showed ovicidal and larvicidal activity against H. contortus. TTO, terp-4-ol and, mainly, nanoTTO may be targeted in in vivo studies, besides being a promising line of research into the control and treatment of veterinary important helminths.
Franciscana dolphins as PCBs marine biomonitors in Argentina, south-west Atlantic Ocean
Authors: Arias Andrés H. , Panebianco María V. , Net Sopheak , Dumoulin David , Souissi Sami , Ouddane Baghdad , Cappozzo Humberto L. , Marcovecchio Jorge E.
Published: June 2015

Including a multi-year collection of samples (2004–2011) the present research fills 20 years of an information gap regarding the PCB burden in south-west Atlantic franciscana dolphins (Pontoporia blainvillei) while aiming to test the null hypothesis that PCBs congeners are increasingly bioaccumulating in south-west Atlantic specimens in relation to northern hemisphere records. In addition, the present survey analyses indicators of potential biological impairment associated to PCBs tissue burden. The results could associate each sampling area group of dolphins to one or two Aroclor® patterns and point to dominant regional diffuse sources entering PCBs mixtures to the marine environment with a possible regional/long-range atmospheric contribution. In addition, total PCB levels were from four to seven times lower than the closer precedents for the area (18–26 years ago) indicating a progress in the environmental release and biota exposure of PCBs and posing an objective indicator of success of the present international elimination programme. Further, when compared with regional and global bioaccumulation patterns, PCBs congeners in Argentinean specimens appeared to occur in a decreasing tendency. Finally, calculated TEQs TCDD levels raised a concern in regards to environmental safety, showing guideline values to be widely exceeded and the occasional occurrence of positive correlations between PCBs bioaccumulationvs. sexual immaturity.
Diversity of shallow water zoantharians in Hengam and Larak Islands, in the Persian Gulf
Authors: Noori Koupaei Atoosa , Mostafavi Pargol Ghavam , Fallah Mehrabadi Jalil , Fatemi Seyed Mohammad Reza , Dehghani Hamed
Published: June 2015

Zoantharians are one of the least studied orders of benthic cnidarians of the Persian Gulf. A survey and molecular analysis was conducted to determine zoantharian species diversity in the Persian Gulf. For this purpose, 63 colonies of zoantharians were collected from Hengam and Larak Islands in the Strait of Hormuz and some morphological characteristics of each specimen were recorded, i.e. sand encrustation, polyp shape and colour, oral groove, oral zone and oral disc colours as well as tentacle number and colour. After DNA extraction, mitochondrial 16S ribosomal DNA (16S rDNA) and cytochrome oxidase subunit I (COI) were amplified by polymerase chain reaction (PCR). Based on obtained 16S rDNA and COI gene sequences, five putative species-level clades were identified:Zoanthus sansibaricus(N = 30),Palythoa tuberculosa(N = 12),Palythoa mutuki(N = 2),Palythoaaff.mutuki(N = 18) andNeozoanthussp. Iran (N = 1). While the first three are known species, the last two were potentially novel undescribed species.Palythoaaff.mutukihas an external appearance similar toPalythoa mutuki. However, mitochondrial DNA sequences obtained from these specimens placed them in a previously undescribed species group. TheNeozoanthusspecimen was morphologically and molecularly different from other describedNeozoanthusspecies. This is the first record of this genus from the Persian Gulf and neighbouring areas. Since there is not much work on zoantharians identification in the Persian Gulf, further sampling and investigation is needed to speculate on the accuracy of these potentially new species and to complete the knowledge of zoantharian diversity in this area.
Life history of Sternaspis costata (Sternaspidae: Polychaeta) in Ariake Bay, Japan
Authors: Yoshino Kenji , Nagayoshi Manato , Sato Masanori , Katano Toshiya , Ito Yuji , Fujii Naoki , Hamada Takaharu , Hayami Yuichi
Published: June 2015

The life history of the Japanese sternaspid polychaeteSternaspis costatavon Marenzeller, 1879 was investigated in the inner part of Ariake Bay from May 2010 to May 2011, with additional sampling in July and September 2011. All the worms were measured by the width of the ventral shield (WS) as an indicator of body size, and their coelomic contents were also examined. Ovigerous females occurred throughout the year, except during October and March, with the highest ovigerous rate (number of ovigerous females/number of adults with a WS larger than that of the smallest ovigerous female, i.e. 1.8 mm) noted in September (43.8% in 2010, 34.7% in 2011). The ovigerous rate was less than 10% in the other months, except in April 2011 (30%). Although the coelomic oocytes ranged from 90 to 160 μm in diameter, the diameters of most of the oocytes were 140–160 μm in September. The number of adults drastically decreased from September to October, suggesting that most of the adults died after spawning in the major reproductive period, although a small fraction of adults seemed to reproduce earlier or later. A cohort of recruits with an approximately 1.2 mm WS was identified in March and April. Most of the recruits grew to adult size in July–September, when many ovigerous females occurred. These results indicated that this species is fundamentally semelparous, with longevity around 1 year, and they mostly reproduce in September, with a few adults reproducing almost throughout the year.
Is the Martinique ground snake Erythrolamprus cursor extinct?
Authors: Caut Stephane , Jowers Michael J.
Published: June 2015

The Caribbean Islands are a biodiversity hotspot where anthropogenic disturbances have had a significant impact, causing population declines and extinction of endemic species. The ground snake Erythrolamprus cursor is a dipsadid endemic to Martinique; it is categorized as Critically Endangered on the IUCN Red List and is known only from museum specimens. The snake was common on Martinique during the 18th and 19th centuries but there have been no reliable sightings since 1968, suggesting it may have gone extinct, probably as a result of the introduction of the small Indian mongoose Herpestes javanicus auropunctatus. However, the islet known as Diamond Rock, south-west of Martinique, is mongoose-free and the last reported sighting of E. cursor there was in 1968. The islet was last occupied during the Napoleonic Empire (the early 19th century), is now completely protected, and is difficult to access (it spans 5.8 ha, with a maximum elevation of 175 m). We conducted the first extensive survey of the islet, over 10 days, to clarifty the status of E. cursor. Our study revealed that unique conditions exist on Diamond Rock (i.e. aridity and a distinct potential prey community) and that E. cursor would have had to modify its ecology to persist on the islet. Although the rugged terrain of Diamond Rock makes it difficult to explore, it is probable that E. cursor is now extinct.
Improved cognitive content endures for 2 years among unstable responders to acute-phase cognitive therapy for recurrent major depressive disorder
Authors: Vittengl J. R. , Clark L. A. , Thase M. E. , Jarrett R. B.
Published: June 2015

Background.

The cognitive model of depression suggests that cognitive therapy (CT) improves major depressive disorder (MDD) in part by changing depressive cognitive content (e.g. dysfunctional attitudes, hopelessness). The current analyses clarified: (1) the durability of improvements in cognitive content made by acute-phase CT responders; (2) whether continuation-phase CT (C-CT) or fluoxetine (FLX) further improves cognitive content; and (3) the extent to which cognitive content mediates continuation treatments’ effects on depressive symptoms and major depressive relapse/recurrence.

Method.

Out-patients with recurrent MDD who responded to acute-phase CT (n = 241) were randomized to 8 months of C-CT, FLX or pill placebo (PBO) and followed for an 24 additional months. Cognitive content was assessed approximately every 4 months using five standard patient-report measures.

Results.

Large improvements in cognitive content made during acute-phase CT were maintained for 32 months, with 78–90% of patients scoring in normal ranges, on average. Cognitive content varied little between C-CT, FLX and PBO arms, overall. Small, transient improvements in cognitive content in C-CT or FLX compared with PBO patients did not clearly mediate the treatments’ effects on depressive symptoms or on major depressive relapse/recurrence.

Conclusions.

Outpatients with recurrent MDD who respond to acute-phase CT show durable improvements in cognitive content. C-CT or FLX may not continue to improve patient-reported cognitive content substantively, and thus may treat recurrent MDD by other paths.
Baby sign but not spontaneous gesture predicts later vocabulary in children with Down Syndrome
Authors: ÖZÇALIŞKAN ŞEYDA , ADAMSON LAUREN B. , DIMITROVA NEVENA , BAILEY JHONELLE , SCHMUCK LAUREN
Published: June 2015

Early spontaneous gesture, specifically deictic gesture, predicts subsequent vocabulary development in typically developing (TD) children. Here, we ask whether deictic gesture plays a similar role in predicting later vocabulary size in children with Down Syndrome (DS), who have been shown to have difficulties in speech production, but strengths in spontaneous gesture and baby sign use. We compared the gestures and baby signs produced by twenty-three children with DS (Mage = 2;6) and twenty-three TD children (Mage = 1;6), in relation to their expressive spoken vocabulary size one year later. Children with DS showed significant deficits in gesture production, particularly for deictic gestures, but strengths in baby sign production, compared to their typically developing peers. More importantly, it was the baby signs produced by children with DS, but not deictic gestures, that predicted their spoken vocabulary size one year later. Our results further highlight the important role baby signs can play in language development in children with developmental disorders.
Seamless Pedestrian Positioning and Navigation Using Landmarks
Authors: Basiri Anahid , Amirian Pouria , Winstanley Adam , Marsh Stuart , Moore Terry , Gales Guillaume
Published: June 2015

Many navigation services, such as car navigation services, provide users with praxic navigational instructions (such as “turn left after 200 metres, then turn right after 150 metres”), however people usually associate directions with visual cues (e.g. “turn right at the square”) when giving navigational instructions in their daily conversations. Landmarks can play an equally important role in navigation and routing services. Landmarks are unique and easy-to-recognise and remember features; therefore, in order to remember when exploring an unfamiliar environment, they would be assets. In addition, Landmarks can be found both indoors and outdoors and their locations are usually fixed. Any positioning techniques which use landmarks as reference points can potentially provide seamless (indoor and outdoor) positioning solutions. For example, users can be localised with respect to landmarks if they can take a photograph of a registered landmark and use an application for image processing and feature extraction to identify the landmark and its location. Landmarks can also be used in pedestrian-specific path finding services. Landmarks can be considered as an important parameter in a path finding algorithm to calculate a route passing more landmarks (to make the user visit a more tourist-focussed area, pass along an easier-to-follow route, etc.). Landmarks can also be used as a part of the navigational instructions provided to users; a landmark-based navigation service makes users sure that they are on the correct route, as the user is reassured by seeing the landmark whose information/picture has just been provided as a part of navigational instruction. This paper shows how landmarks can help improve positioning and praxic navigational instructions in all these ways.
Sweet corn hybrid tolerance to weed competition under three weed management levels
Authors: Boydston R.A. , Williams M.M.
Published: June 2015

Nearly all commercial sweet corn fields contain weeds that escaped management and, therefore, sweet corn often suffers yield losses due to weed competition. For this reason, field trials were conducted from 2009 to 2011 near Prosser, WA and Urbana, IL to evaluate the responses of weeds and four sweet corn hybrids to three levels of weed management; weed free, high intensity cultivation (HC), and low intensity cultivation (LC). Weed management level had the greatest impact on early season weed densities and HC reduced final weed biomass more than LC in 2 of 4 site-years. Two taller sweet corn hybrids with greater leaf area suppressed final weed biomass more than two shorter hybrids with less leaf area in 3 of 4 site-years. When grown with less intense weed management that resulted in more weeds, taller sweet corn hybrids with greater leaf area maintained yields better than shorter, less competitive sweet corn hybrids. Utilizing hybrids with greater tolerance to weeds and greater ability to suppress weeds could be a valuable component of an integrated weed management system.
Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1–BAP1 complex
Authors: Balasubramani Anand , Larjo Antti , Bassein Jed A. , Chang Xing , Hastie Ryan B. , Togher Susan M. , Lähdesmäki Harri , Rao Anjana
Published: June 2015

ASXL1 is the obligate regulatory subunit of a deubiquitinase complex whose catalytic subunit is BAP1. Heterozygous mutations of ASXL1 that result in premature truncations are frequent in myeloid leukemias and Bohring–Opitz syndrome. Here we demonstrate that ASXL1 truncations confer enhanced activity on the ASXL1–BAP1 complex. Stable expression of truncated, hyperactive ASXL1–BAP1 complexes in a haematopoietic precursor cell line results in global erasure of H2AK119Ub, striking depletion of H3K27me3, selective upregulation of a subset of genes whose promoters are marked by both H2AK119Ub and H3K4me3, and spontaneous differentiation to the mast cell lineage. These outcomes require the catalytic activity of BAP1, indicating that they are downstream consequences of H2AK119Ub erasure. In bone marrow precursors, expression of truncated ASXL1–BAP1 complex cooperates with TET2 loss-of-function to increase differentiation to the myeloid lineage in vivo. Our data raise the possibility that ASXL1 truncation mutations confer gain-of-function on the ASXL–BAP1 complex.