bluuebunny/crossref_metadata_2025 huggingface dataset:
Multidimensional characteristics, prognostic role, and preoperative prediction of peritoneal sarcomatosis in retroperitoneal sarcoma
Authors: Li Yang , Wu Jian-Hui , Li Cheng-Peng , Liu Bo-Nan , Tian Xiu-Yun , Qiu Hui , Hao Chun-Yi , Lv Ang
Published: October 2022

Background

Peritoneal sarcomatosis (PS) could occur in patients with retroperitoneal sarcomas (RPS). This study aimed to expand the understanding of PS on its characteristics and prognostic role, and develop a nomogram to predict its occurrence preoperatively.

Methods

Data of 211 consecutive patients with RPS who underwent surgical treatment between 2011 and 2019 was retrospectively reviewed. First, the clinicopathological characteristics of PS were summarized and analyzed. Second, the disease-specific survival (DSS) and recurrence-free survival (RFS) of patients were analyzed to evaluate the prognostic role of PS. Third, preoperative imaging, nearly the only way to detect PS preoperatively, was combined with other screened risk factors to develop a nomogram. The performance of the nomogram was assessed.

Results

Among the 211 patients, 49 (23.2%) patients had PS with an incidence of 13.0% in the primary patients and 35.4% in the recurrent patients. The highest incidence of PS occurred in dedifferentiated liposarcoma (25.3%) and undifferentiated pleomorphic sarcoma (25.0%). The diagnostic sensitivity of the preoperative imaging was 71.4% and its specificity was 92.6%. The maximum standardized uptake value (SUVmax) was elevated in patients with PS (P<0.001). IHC staining for liposarcoma revealed that the expression of VEGFR-2 was significantly higher in the PS group than that in the non-PS group (P = 0.008). Survival analysis (n =196) showed significantly worse DSS in the PS group than in non-PS group (median: 16.0 months vs. not reached, P < 0.001). In addition, PS was proven as one of the most significant prognostic predictors of both DSS and RFS by random survival forest algorithm. A nomogram to predict PS status was developed based on preoperative imaging combined with four risk factors including the presentation status (primary vs. recurrent), ascites, SUVmax, and tumor size. The nomogram significantly improved the diagnostic sensitivity compared to preoperative imaging alone (44/49, 89.8% vs. 35/49, 71.4%). The C-statistics of the nomogram was 0.932, and similar C-statistics (0.886) was achieved at internal cross-validation.

Conclusion

PS is a significant prognostic indicator for RPS, and it occurs more often in recurrent RPS and in RPS with higher malignant tendency. The proposed nomogram is effective to predict PS preoperatively.
The path towards consensus genome classification of diffuse large B-cell lymphoma for use in clinical practice
Authors: Mendeville Matias , Roemer Margaretha G. M. , Los-de Vries G. Tjitske , Chamuleau Martine E. D. , de Jong Daphne , Ylstra Bauke
Published: October 2022

Diffuse large B-cell lymphoma (DLBCL) is a widely heterogeneous disease in presentation, treatment response and outcome that results from a broad biological heterogeneity. Various stratification approaches have been proposed over time but failed to sufficiently capture the heterogeneous biology and behavior of the disease in a clinically relevant manner. The most recent DNA-based genomic subtyping studies are a major step forward by offering a level of refinement that could serve as a basis for exploration of personalized and targeted treatment for the years to come. To enable consistent trial designs and allow meaningful comparisons between studies, harmonization of the currently available knowledge into a single genomic classification widely applicable in daily practice is pivotal. In this review, we investigate potential avenues for harmonization of the presently available genomic subtypes of DLBCL inspired by consensus molecular classifications achieved for other malignancies. Finally, suggestions for laboratory techniques and infrastructure required for successful clinical implementation are described.
BgNet: Classification of benign and malignant tumors with MRI multi-plane attention learning
Authors: Liu Hong , Jiao Meng-Lei , Xing Xiao-Ying , Ou-Yang Han-Qiang , Yuan Yuan , Liu Jian-Fang , Li Yuan , Wang Chun-Jie , Lang Ning , Qian Yue-Liang , Jiang Liang , Yuan Hui-Shu , Wang Xiang-Dong
Published: October 2022

Objectives

To propose a deep learning-based classification framework, which can carry out patient-level benign and malignant tumors classification according to the patient’s multi-plane images and clinical information.

Methods

A total of 430 cases of spinal tumor, including axial and sagittal plane images by MRI, of which 297 cases for training (14072 images), and 133 cases for testing (6161 images) were included. Based on the bipartite graph and attention learning, this study proposed a multi-plane attention learning framework, BgNet, for benign and malignant tumor diagnosis. In a bipartite graph structure, the tumor area in each plane is used as the vertex of the graph, and the matching between different planes is used as the edge of the graph. The tumor areas from different plane images are spliced at the input layer. And based on the convolutional neural network ResNet and visual attention learning model Swin-Transformer, this study proposed a feature fusion model named ResNetST for combining both global and local information to extract the correlation features of multiple planes. The proposed BgNet consists of five modules including a multi-plane fusion module based on the bipartite graph, input layer fusion module, feature layer fusion module, decision layer fusion module, and output module. These modules are respectively used for multi-level fusion of patient multi-plane image data to realize the comprehensive diagnosis of benign and malignant tumors at the patient level.

Results

The accuracy (ACC: 79.7%) of the proposed BgNet with multi-plane was higher than that with a single plane, and higher than or equal to the four doctors’ ACC (D1: 70.7%, p=0.219; D2: 54.1%, p<0.005; D3: 79.7%, p=0.006; D4: 72.9%, p=0.178). Moreover, the diagnostic accuracy and speed of doctors can be further improved with the aid of BgNet, the ACC of D1, D2, D3, and D4 improved by 4.5%, 21.8%, 0.8%, and 3.8%, respectively.

Conclusions

The proposed deep learning framework BgNet can classify benign and malignant tumors effectively, and can help doctors improve their diagnostic efficiency and accuracy. The code is available at https://github.com/research-med/BgNet.
Cost-benefit analysis of the integrated pharmaceutical supply chain information service after the establishment of the Korean Pharmaceutical Information Service
Authors: Kim Myojeong , Lee GeunWoo , Hwang Yungi , Kim Tae Hyun , Kim Dong-Sook
Published: October 2022

Background: The Korean Pharmaceutical Information Service (KPIS) was established in October 2007 to increase the transparency of the pharmaceutical supply chain by integrating relevant information. This study aimed to describe the KPIS program and perform a cost-benefit analysis of the KPIS.

Methods: We conducted a cost-benefit analysis based on cost savings in terms of National Health Insurance (NHI). The outcome measures were the net financial benefit and benefit-cost ratio over the 12 years since the establishment of the KPIS. The cost estimate included the costs of labor and business operations, the development of an information entry system, and office maintenance. Financial benefits were defined as savings resulting from the implementation of the program based on KPIS data. Social benefits were defined as the prevention of recalled medicines from entering the supply chain and the decrease in inventory and disposal.

Results: The KPIS clearly resulted in a net financial benefit, saving 37.2 million USD, which was 2.6 times higher than the cost of implementation. While the benefit-cost ratio was less than one during the first period, it exceeded 3.4 during the second period. After calculating and integrating social benefits, the net benefit increased to 571.6 million USD, and the benefit-cost ratio was 24.8. A sensitivity analysis of the annual benefit showed that the net benefit varied from a low of −1.5 million USD to a high of 24.7 million USD according to the program implementation year.

Conclusion: The establishment of the KPIS and a system for collecting information on the pharmaceutical supply chain showed meaningful financial and social benefits when compared to the input cost. Since no other countries have an integrated pharmaceutical information system that incorporates all information from production to administration, the example of the KPIS can provide a precedent for other countries.
Transcriptome and metabolome analyses provide insights into the relevance of pericarp thickness variations in Camellia drupifera and Camellia oleifera
Authors: Li Yongjuan , Liao Boyong , Wang Yi , Luo Huihua , Wang Shimin , Li Caiqin , Song Wenpei , Zhang Kunchang , Yang Boqun , Lu Shaoqiang , Zhang Bipei , Li Yongquan
Published: October 2022

Camellia fruit is a woody edible oil source with a recalcitrant pericarp, which increases processing costs. However, the relevance of pericarp thickness variations in Camellia species remains unclear. Therefore, this study aimed to identify pericarp differences at the metabolic and transcription levels between thick-pericarp Camellia drupifera BG and thin-pericarp Camellia oleifera SG. Forty differentially accumulated metabolites were screened through non-targeted UHPLC-Q-TOF MS-based metabolite profiling. S-lignin was prominently upregulated in BG compared with SG, contributing to the thick pericarp of BG. KEGG enrichment and coexpression network analysis showed 29 differentially expressed genes associated with the lignin biosynthetic pathway, including 21 genes encoding catalysts and 8 encoding transcription factors. Nine upregulated genes encoding catalysts potentially led to S-lignin accumulation in BG pericarp, and transcription factors NAC and MYB were possibly involved in major transcriptional regulatory mechanisms. Conventional growth-related factors WRKYs and AP2/ERFs were positively associated while pathogenesis-related proteins MLP328 and NCS2 were negatively associated with S-lignin content. Thus, Camellia balances growth and defense possibly by altering lignin biosynthesis. The results of this study may guide the genetic modifications of C. drupifera to optimize its growth–defense balance and improve seed accessibility.
Characterization and attribution of vegetation dynamics in the ecologically fragile South China Karst: Evidence from three decadal Landsat observations
Authors: Pei Jie , Wang Li , Huang Huabing , Wang Lei , Li Wang , Wang Xiaoyue , Yang Hui , Cao Jianhua , Fang Huajun , Niu Zheng
Published: October 2022

Plant growth and its changes over space and time are effective indicators for signifying ecosystem health. However, large uncertainties remain in characterizing and attributing vegetation changes in the ecologically fragile South China Karst region, since most existing studies were conducted at a coarse spatial resolution or covered limited time spans. Considering the highly fragmented landscapes in the region, this hinders their capability in detecting fine information of vegetation dynamics taking place at local scales and comprehending the influence of climate change usually over relatively long temporal ranges. Here, we explored the spatiotemporal variations in vegetation greenness for the entire South China Karst region (1.9 million km2) at a resolution of 30m for the notably increased time span (1987-2018) using three decadal Landsat images and the cloud-based Google Earth Engine. Moreover, we spatially attributed the vegetation changes and quantified the relative contribution of driving factors. Our results revealed a widespread vegetation recovery in the South China Karst (74.80%) during the past three decades. Notably, the area of vegetation recovery tripled following the implementation of ecological engineering compared with the reference period (1987-1999). Meanwhile, the vegetation restoration trend was strongly sustainable beyond 2018 as demonstrated by the Hurst exponent. Furthermore, climate change contributed only one-fifth to vegetation restoration, whereas major vegetation recovery was highly attributable to afforestation projects, implying that anthropogenic influences accelerated vegetation greenness gains in karst areas since the start of the new millennium during which ecological engineering was continually established. Our study provides additional insights into ecological restoration and conservation in the highly heterogeneous karst landscapes and other similar ecologically fragile areas worldwide.
Integrated analysis reveals effects of bioactive ingredients from Limonium Sinense (Girard) Kuntze on hypoxia-inducible factor (HIF) activation
Authors: Zhao Hualong , Wang Siyuan , Zhou Yilu , Ertay Ayse , Williamson Philip T. F. , Ewing Rob M. , Tang Xinhui , Wang Jialian , Wang Yihua
Published: October 2022

Limonium Sinense (Girard) Kuntze is a traditional Chinese medicinal herb, showing blood replenishment, anti-tumour, anti-hepatitis, and immunomodulation activities amongst others. However, the mechanism of its pharmacological activities remains largely unknown. Here, we investigated the effects of bioactive ingredients from Limonium Sinense using an integrated approach. Water extracts from Limonium Sinense (LSW) showed a strong growth inhibitory effect on multiple cells in both 2D and 3D cultures. Global transcriptomic profiling and further connectivity map (CMap) analysis identified several similarly acting therapeutic candidates, including Tubulin inhibitors and hypoxia-inducible factor (HIF) modulators. The effect of LSW on the cell cycle was verified with flow cytometry showing a G2/M phase arrest. Integrated analysis suggested a role for gallic acid in mediating HIF activation. Taken together, this study provides novel insights into the bioactive ingredients in Limonium Sinense, highlighting the rich natural resource and therapeutic values of herbal plants.
Stardust and feminism: A creatureliness agenda
Authors: Dess Nancy K.
Published: October 2022

People are living, breathing creatures. Dominant feminist discourses are situated within hegemonic human exceptionalism (HHE) which, by framing the body in terms of human forms of meaning-making and social life, eschews first-order embodiment (or creatureliness) as worthy of inquiry. Here, well-known reasons for avoidance of “the biological” are briefly summarized and an argument is advanced for meta-theoretical centering of creatureliness. A three-pronged agenda is proposed that embraces the creaturely body without the “-isms” (e.g., essentialism) and “-izings” (e.g., so-called “naturalizing”) that subvert feminist commitments. By unsettling HHE, executing the agenda would promote broader feminist coalitions and new scholarly collaborations aimed at fleshing out gender.
Green advertising is more environmentally friendly? The influence of advertising color on consumers’ preferences for green products
Authors: Wenting Feng , Yuelong Zeng , Xianyun Shen , Chenling Liu
Published: October 2022

The color of green product advertisements is an important factor affecting consumers’ preferences. Based on the theory of the self-control system, this paper explores the influence mechanism and boundary conditions of green product ad color on consumers’ preferences through three experiments. Experiment 1 tested the effect of advertisement color type (green/color) on consumers’ preferences for green products. The results show that color ad can promote consumers’ preferences for green products compared with green ad. Experiment 1 also analyzed the mediating role of the self-control system between advertisement color type (green/color) and consumers’ preferences. Experiment 2 further clarified the boundary of the main effect. The effect of ad color (green/color) on consumers’ preferences was only effective in the context of green products. Experiment 3 explored the moderating effect of green product type (egoistic/altruistic) on the main effect. The results show that only when the green product type is altruistic, the ad color type (green/color) can significantly affect consumers’ preferences. This study is the first to link the ad color of green products with consumers’ preferences. The findings confirm that the use of color ad for green products can elicit higher consumers’ preferences than pure green ad, which enriches the research on the color of green product advertisements.
Healthcare graduate students' perceived control and preventive behavior for COVID-19 in Japan and the United States: A cross-sectional study
Authors: Abuliezi Renaguli , Kondo Akiko , Niitsu Kosuke , Ota Erika
Published: October 2022

Background

Both individual and policy level perceived control are known to be positively related to preventive behavior, and both may differ among healthcare graduate students with different cultural backgrounds. This study compared the preventive health behavior and perceived control among domestic and international healthcare graduate students in Japan and the United States during the COVID-19 pandemic, and analyzed factors associated with preventive health behavior and perceived control.

Methods

The study used a self-administered online survey, conducted at two universities in Japan and one university in the United States. The survey included sociodemographic data and scales of preventive health behaviors, perceived control (policy level), and perceived health competence (individual level). Association among variables were analyzed using structural equation modeling.

Results

A total of 610 students (485 domestic and 125 international) in Japan and 231 students (220 domestic and 11 international) in the United States completed the survey. Participants' average age was 31.3 years, and 67.0% were female. Model fit of structural equation modeling was good (χ2 = 9.419, P = 0.151, comparative fit index = 0.995, RMSEA = 0.026). Japanese students had better preventive health behavior than American (β = −0.407, P < 0.001) and international students in both countries (β = −0.112, P < 0.001). However, Japanese students had significantly lower perceived control than American students (β = 0.346, P < 0.001) and international students in both countries (β = 0.188, P < 0.001). Overall higher perceived control (β = 0.175, P < 0.001) and being female (β = 0.141, P < 0.001) were significantly associated with better preventive behavior. Although higher perceived control was related to higher perceived health competence (β = 0.295, P < 0.001), perceived health competence was not associated with preventive behavior (β = 0.025, P = 0.470). Religion was not associated with perceived control or preventive behavior.

Conclusion

Nationality was identified as the main factor associated with both perceived control and preventive behavior. Policy level perceived control was more strongly associated with preventive health behavior than individual level perceived health competence. Further investigations in the contribution of specific cultural dimensions associated with perceived control and preventive behaviors are recommended.
Extraction, analysis, and occurrence of per- and polyfluoroalkyl substances (PFAS) in wastewater and after municipal biosolids land application to determine agricultural loading
Authors: Caniglia Justin , Snow Daniel D. , Messer Tiffany , Bartelt-Hunt Shannon
Published: October 2022

Given the ubiquitous detection of per- and polyfluoroalkyl substances (PFAS) within numerous soil and water environmental compartments, there is a need for global understanding of current methodologies for extracting water, solids, polar organic chemical integrative samplers (POCIS), and plant tissue for these substances. This study provides details of several current extraction methods, demonstrates the use of POCIS in monitoring these compounds in a wastewater environment, and provides evidence of detectable levels of certain PFAS compounds within Midwestern municipalities and agroecosystems. Validated extraction procedures help characterize occurrence and release of 18 PFAS in a midwestern wastewater treatment plant (WWTP), surface water, runoff after land application of biosolids to agricultural test plots, infiltration into topsoil, and uptake by grain sorghum. Of the compounds measured, 14 PFAS were detected at least at one sampling site or type. The average total (Σ PFAS) dissolved phase time-weighted average (TWA) concentration in wastewater influent, effluent and in the upstream and downstream effluent mixing zone (EMZ) sites in the receiving stream, respectively, were 27.9, 132, 37.7, and 71.4 ng L−1. Long-chain PFAS dominated most of the aqueous compartments, and perfluoroalkyl acids (PFAAs) occurred in the WWTP and receiving surface waters. Total Σ14 PFAS measured in municipal biosolids applied to soils were 22.9 ng g−1 dw with long-chain PFAS comprising 77.5% of the cumulative PFAS mass. Perfluorooctanesulfonic acid (PFOS) was the most abundant compound detected in biosolids at the highest concentration (9.40 ng g−1 dw). Accumulation in WWTP biosolids was estimated to occur at a rate of 72.8 g day−1 dw based on the difference between influent and effluent time weighted average concentrations. PFAS were detected in both surface soil and runoff after land application of biosolids, but also in control plots consistent with background PFAS contamination. PFAS concentrations in surface runoff decreased over time from plots treated with biosolids. These results provide evidence of the introduction of PFAS to agroecosystems from wastewater effluent and land application of biosolids in the Midwest.
The colibri telescope array for KBO detection through serendipitous stellar occultations: A technical description
Authors: Mazur Michael J. , Metchev Stanimir , Brown Rachel A. , Gupta Ridhee , Bloch Richard , Mills Tristan , Pass Emily
Published: October 2022

We present the technical design, construction and testing of the Colibri telescope array at Elginfield Observatory near London, Ontario, Canada. Three 50-cm telescopes are arranged in a triangular array and are separated by 110–160 m. During operation, they will monitor field stars at the intersections of the ecliptic and galactic plane for serendipitous stellar occultations (SSOs) by trans-Neptunian objects (TNOs). At a frame rate of 40 frames per second (fps), Fresnel diffraction in the occultation light curve can be resolved and, with coincident detections, be used to estimate basic properties of the occulting object. Using off-the-shelf components, the Colibri system streams imagery to disk at a rate of 1.5 GB/s for next-day processing by a custom occultation detection pipeline.

The imaging system has been tested and is found to perform well, given the moderate site conditions. Limiting magnitudes at 40 fps are found to be about 12.1 (temporal SNR = 5, visible light Gaia G band) with time-series standard deviations ranging from about 0.035 mag to >0.2 mag. SNR is observed to decrease linearly with magnitude for stars fainter than about G = 9.5 mag. Brighter than this limit, SNR is constant, suggesting that atmospheric scintillation is the dominant noise source. Astrometric solutions show errors typically less than ±0.3 pixels (0.8 arc seconds) without a need for high-order corrections.
Use of platelet-rich fibrin in fat grafts during facial lipostructure
Authors: Zhang Zhaoxiang , Qiu Lihong , Cui Dong , Geng Jian , Yi Chenggang
Published: October 2022

Background

This review was designed to discuss the safety and efficacy of using platelet-rich fibrin (PRF) in fat grafts during facial lipostructure.

Methods

From January 2018 to December 2021, 650 fat grafts for facial lipostructure were performed in the authors' department. According to their wishes, we divided the patients into two groups: 498 patients were treated with autologous fat injection (control group), and 152 patients were treated with autologous fat injection combined with PRF. All of the patients were monitored for at least six months. The effects were evaluated via physician assessment and patient satisfaction rates, and the incidences of complications were compared.

Results

All the cases had a degree of improvement after treatment. The patient satisfaction rate was 55.3% in the PRF group and 43.4% in the control group. In all, 68.4% of the patients in the PRF group and 58.2% in the control group indicated that one-stage surgery was sufficient to achieve the desired effect. According to the evaluation conducted by the plastic surgeon, 59.2% of patients in the PRF group and 47.0% in the control group achieved a perfect effect. A total of 76.3% of patients in the PRF group and 63.9% in the control group reported that one surgery achieved satisfactory results. The difference between the PRF and control groups was statistically significant.

Conclusion

Using an autologous fat graft during facial lipostructure is beneficial and safe when combined with PRF. The combination may enhance the effect and satisfaction rate. Further research and prospective clinical studies are needed to understand the role of PRF in fat grafting.
Microbiota of vaccinated and non-vaccinated clinically inconspicuous and conspicuous piglets under natural Lawsonia intracellularis infection
Authors: Hankel Julia , Sander Saara , Muthukumarasamy Uthayakumar , Strowig Till , Kamphues Josef , Jung Klaus , Visscher Christian
Published: October 2022

Lawsonia(L.)intracellularisis a widespread, economically important bacterium causing the porcine proliferative enteropathy (PPE). In this study, we evaluated intestinal microbiota of naturally exposedL. intracellularis-positive pigs under standardized conditions. To obtain three independent repetitions, 27L. intracellularis-infected pigs (19.0 ± 1.50 kg body weight) from one farm were divided into three groups at an age of 7 to 8 weeks (nine pigs/group). Pigs were either vaccinated againstL. intracellularisvia oral drenching on their 21st day of life (attenuated live vaccine) or non-vaccinated and selected according to clinical findings (pigs without deviating fecal consistency or with moderate to soft fecal consistency). Comparison of the clinically inconspicuous piglets that differed regarding their vaccination status showed fewer significant differences in fecal microbiota composition. The vaccination led to an overall enrichment of bacterial species belonging to the orderClostridiales, while species of the genusCollinsellaandPrevotellawere decreased. Several bacterial species belonging to the orderBacteroidales, mainly of the familyPrevotellacecae, often closely matchingPrevotella copridiffered significantly between non-vaccinated clinically inconspicuous and conspicuous piglets. Whether those bacterial species play a role in mitigating the severity of anL. intracellularisinfection remains to be defined.
Geoprivacy in Neighbourhoods and Health Research: A Mini-Review of the Challenges and Best Practices in Epidemiological Studies
Authors: Ribeiro Ana Isabel , Dias Vasco , Ribeiro Sofia , Silva José Pedro , Barros Henrique
Published: October 2022

Neighbourhood and health research often relies on personal location data (e.g., home address, daily itineraries), despite the risks of geoprivacy breaches. Thus, geoprivacy is an important emerging topic, contemplated in international regulations such as the General Data Protection Regulation. In this mini-review, we briefly assess the potential risks associated with the usage of personal location data and provide geoprivacy-preserving recommendations to be considered in epidemiological research. Risks include inference of personal information that the individual does not wish to disclose, reverse-identification and security breaches. Various measures should be implemented at different stages of a project (pre-data collection, data processing, data analysis/publication and data sharing) such as informed consent, pseudo-anonymization and geographical methods.
CEO Education and Firm Performance: Evidence from Corporate Universities
Authors: Nguyen Hai Long , Fan Pengda
Published: October 2022

Corporate universities are increasingly being established in response to corporate dissatisfaction with the knowledge provided by traditional universities. While some argue that they may pose the greatest threat to traditional universities, others propose that corporate universities might provide better undergraduate-level education than traditional universities. To this end, this research tries to answer the following research question: can integrating corporation education into traditional universities provide better educational outcomes? Using a sample of 40,563 firm-year observations from 2001 to 2019 and exploiting the unique setting of Japanese corporate universities, the authors find that the CEOs who are graduates of corporate universities significantly underperform compared to their counterparts graduating from traditional universities. The results are robust under various alternative estimations, such as general method of moments (GMM) IV regressions and propensity score matching. Overall, the results indicate that integrating corporation needs into traditional higher education will lead to lower individual productivity.
Establishment of a Protoplasts-Based Transient Expression System in Banana (Musa spp.)
Authors: Zhao Chunhui , Li Shuyu , Du Chanjuan , Gao Hui , Yang Di , Fu Gang , Cui Haitao
Published: October 2022

The breeding of triploid banana cultivars with improved traits, such as yield and disease resistance, remains a major challenge for breeders. One reason is that the molecular study and functional gene analysis in bananas fall behind due to the difficulties of its genetic manipulation. The plant protoplast-based transient transformation has been documented and widely used as a versatile and convenient system for functional gene analysis in many plant species. However, an efficient high-quality protoplast isolation and transformation system is still lacking for bananas. Here, we established an efficient protoplast isolation and transformation method for bananas by selecting proper source materials, optimizing conditions for enzymatic hydrolysis and PEG-mediated transfection. We found the best source materials for banana protoplasts’ isolation are young suckers, which give a yield of protoplasts ranging from 2.5 × 106 to 10.1 × 107 g−1 fresh weight after 5 to 6 h of enzymolysis. The yield is sufficient for most assays that have been established in protoplasts-based systems, such as protein subcellular localization and protein interaction assays. Moreover, using the established transient gene expression system in banana protoplasts, we validated the subcellular localization of Arabidopsis VESICLE SORTING RECEPTOR 1 (VSR1) and the protein self-interaction of Arabidopsis CNGC20 on the cell membrane. The results indicated this system works well and could be routinely used for the functional characterization of banana genes.
Vitamin D Mitigates Hyperglycemia-Induced Cognition Decline in Danio rerio (Zebrafish) through the Activation of Antioxidant Mechanisms
Authors: Uthaiah Chinnappa A. , Devaru Nandini C. , Shivakumar Nandini H. , R Rajalakshmi , Madhunapantula SubbaRao V.
Published: October 2022

Hyperglycemia contributes to the development of cognition impairment and related disorders, induces oxidative stress in neuronal cells; thereby, impairs normal signaling mechanisms involved in cognition processes. Studies have shown a significant decrease in the vitamin D in individuals with hyperglycemia and cognition impairment. But whether supplementing vitamin D has any beneficiary impact on mitigating hyperglycemia-induced cognition impairment is unknown. We have first tested the impact of hyperglycemia on the induction of cognition deficiency in a zebrafish model. Next, the molecular mechanisms related to oxidative stress, which are deregulated in hyperglycemic zebrafish brains, have been explored. Subsequently, the impact of supplementing the water with vitamin D and a known activator of nuclear factor erythroid-2 related factor 2 (Nrf2) i.e., sulforaphane (SFN) on learning and memory functions were assessed. We showed a significant increase in the oxidative stress in the brain tissue of zebrafish residing in hyperglycemic water (111 mM glucose). Addition of vitamin D and SFN increased Nrf2, but differentially modulated its target genes (NQO1, SOD, GPx etc) activity in zebrafish and neuronal cell lines thereby improved the hyperglycemia-induced decline of cognition impairment. Mechanistically, vitamin D binds to the Keap1 protein; thereby, interfering with its binding to Nrf2, which leads to the activation of antioxidant mechanisms in the cells. In summary, reducing the oxidative stress through vitamin D treatment is a possible option for controlling the cognition impairment in diabetic population, but studies testing this possibility in clinical trials are currently needed.
Advanced Coatings of Polyureas for Building Blast Protection: Physical, Chemical, Thermal and Mechanical Characterization
Authors: Leite Fernando , Mota Carlos , Bessa João , Cunha Fernando , Fangueiro Raul , Gomes Gabriel , Mingote José
Published: October 2022

Due to the increase in the global terrorist threat, there has been a growing demand for materials that can more efficiently protect civil, industrial, and military structures against explosions. In this sense, two new commercial polyureas (A and B), that present high potential to be used as a protective coating on building facades against explosions, were compared in this work, through several tests. Chemical characterization with the Scanning Electron Microscope (SEM) of the surface of the polyureas revealed that the commercial polyurea A has a heterogeneous surface while the other polyurea has a more uniform and homogeneous surface, resulting in a more compact structure. The shock-wave attenuation ability of polyurea is believed to be controlled primarily by the hard domains. The TGA tests revealed that polyurea B has more hard segments than polyurea A in its composition. The mechanical tests performed showed that polyurea B has significantly better tensile properties-almost 3000% of maximum deformation capacity compared with approximately 115% of polyurea A. Thus, it was concluded that polyurea B has more potential to be used as a coating in building blast protection due to its exceptional elongation characteristics, a critical parameter to absorb the high frequency and intensity of blasts.
Bioengineering to Accelerate Biodiesel Production for a Sustainable Biorefinery
Authors: Rathore Dheeraj , Sevda Surajbhan , Prasad Shiv , Venkatramanan Veluswamy , Chandel Anuj Kumar , Kataki Rupam , Bhadra Sudipa , Channashettar Veeranna , Bora Neelam , Singh Anoop
Published: October 2022

Biodiesel is an alternative, carbon-neutral fuel compared to fossil-based diesel, which can reduce greenhouse gas (GHGs) emissions. Biodiesel is a product of microorganisms, crop plants, and animal-based oil and has the potential to prosper as a sustainable and renewable energy source and tackle growing energy problems. Biodiesel has a similar composition and combustion properties to fossil diesel and thus can be directly used in internal combustion engines as an energy source at the commercial level. Since biodiesel produced using edible/non-edible crops raises concerns about food vs. fuel, high production cost, monocropping crisis, and unintended environmental effects, such as land utilization patterns, it is essential to explore new approaches, feedstock and technologies to advance the production of biodiesel and maintain its sustainability. Adopting bioengineering methods to produce biodiesel from various sources such as crop plants, yeast, algae, and plant-based waste is one of the recent technologies, which could act as a promising alternative for creating genuinely sustainable, technically feasible, and cost-competitive biodiesel. Advancements in genetic engineering have enhanced lipid production in cellulosic crops and it can be used for biodiesel generation. Bioengineering intervention to produce lipids/fat/oil (TGA) and further their chemical or enzymatic transesterification to accelerate biodiesel production has a great future. Additionally, the valorization of waste and adoption of the biorefinery concept for biodiesel production would make it eco-friendly, cost-effective, energy positive, sustainable and fit for commercialization. A life cycle assessment will not only provide a better understanding of the various approaches for biodiesel production and waste valorization in the biorefinery model to identify the best technique for the production of sustainable biodiesel, but also show a path to draw a new policy for the adoption and commercialization of biodiesel.
The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics
Authors: Monzani Romina , Gagliardi Mara , Clemente Nausicaa , Saverio Valentina , Pańczyszyn Elżbieta , Santoro Claudio , Yissachar Nissan , Visciglia Annalisa , Pane Marco , Amoruso Angela , Corazzari Marco
Published: October 2022

Background: IBD is a spectrum of pathologies characterized by dysregulated immune activation leading to uncontrolled response against the intestine, thus resulting in chronic gut inflammation and tissue damage. Due to its complexity, the molecular mechanisms responsible for disease onset and progression are still elusive, thus requiring intense research effort. In this context, the development of models replicating the etiopathology of IBD and allowing the testing of new potential therapies is critical. Methods: Colon from C57BL/6 or BALB/c mice was cultivated in a Gut-Ex-Vivo System (GEVS), exposed for 5 h to DNBS 1.5 or 2.5 mg/mL, in presence or absence of two probiotic formulations (P1 = Bifidobacterium breve BR03 (DSM16604) and B632 (DSM24706); P2 = Lacticaseibacillus rhamnosus LR04 (DSM16605), Lactiplantibacillus plantarum LP14 (DSM33401) and Lacticaseibacillus paracasei LPC09), and the main hallmarks of IBD were evaluated. Results: Gene expression analysis revealed the following DNBS-induced effects: (i) compromised tight junction organization, responsible for tissue permeability dysregulation; (ii) induction of ER stress, and (iii) tissue inflammation in colon of C57BL/6 mice. Moreover, the concomitant DNBS-induced apoptosis and ferroptosis pathways were evident in colon from both BALB/c and C57BL/6 mice. Finally, the co-administration of probiotics completely prevented the detrimental effects of DNBS. Conclusions: Overall, we have provided results demonstrating that GEVS is a consistent, reliable, and cost-effective system for modeling DNBS-induced IBD, useful for studying the onset and progression of human disease at the molecular level, while also reducing animal suffering. Moreover, we have confirmed the beneficial effect of probiotics administration in promoting the remission of IBD.
Calibrating a Comprehensive Immune Age Metric to Analyze the Cross Sectional Age-Related Decline in Cardiorespiratory Fitness
Authors: Bröde Peter , Claus Maren , Gajewski Patrick D. , Getzmann Stephan , Golka Klaus , Hengstler Jan G. , Wascher Edmund , Watzl Carsten
Published: October 2022

Cardiorespiratory fitness (CRF) is essential for sustained work ability in good health, but declines with aging, as does the functionality of the immune system, the latter process commonly referred to as immunosenescence. This study aimed to compare the capacity of immunosenescence biomarkers with chronological age for predicting low CRF in a cross-sectional sample recruited from the regional working population. CRF was determined by submaximal bicycle ergometer testing in a cross-sectional sample of 597 volunteers aged 20–70 years from the ’Dortmund Vital Study’ (DVS, ClinicalTrials.gov Identifier: NCT05155397). Low CRF was scored if the ergometer test was not completed due to medical reasons or if the power output projected to a heart rate of 130 bpm divided by body mass was below sex-specific reference values of 1.25 W/kg for females and 1.5 W/kg for males, respectively. In addition to established biomarkers of immunosenescence, we calibrated a comprehensive metric of immune age to our data and compared its predictive capacity for low CRF to chronological age, while adjusting our analysis for the influence of sex, obesity, and the level of regular physical activity, by applying univariate and multiple logistic regression. While obesity, low physical activity, chronological and immune age were all associated with increased probability for low CRF in univariate analyses, multiple logistic regression revealed that obesity and physical activity together with immune age, but not chronological age, were statistically significant predictors of low CRF outcome. Sex was non-significant due to the applied sex-specific reference values. These results demonstrate that biological age assessed by our immunological metric can outperform chronological age as a predictor for CRF and indicate a potential role for immunosenescence in explaining the inter-individual variability of the age-related decline in cardiorespiratory fitness.
Proline-Rich Region II (PRR2) Plays an Important Role in Tau–Glycan Interaction: An NMR Study
Authors: Murray Anqesha , Yan Lufeng , Gibson James M. , Liu Jian , Eliezer David , Lippens Guy , Zhang Fuming , Linhardt Robert J. , Zhao Jing , Wang Chunyu
Published: October 2022

(1) Background: Prion-like transcellular spreading of tau pathology in Alzheimer’s disease (AD) is mediated by tau binding to the cell-surface glycan heparan sulfate (HS). However, the structural determinants for tau–HS interaction are not well understood. (2) Methods and Results: Binding-site mapping using NMR showed two major binding regions in full-length tau responsible for heparin interaction. Thus, two tau constructs, tau PRR2* and tau R2*, were designed to investigate the molecular details at the tau–heparin binding interface. The 2D 1H-15N HSQC of tau PRR2* and tau R2* lacked dispersion, which is characteristic for intrinsically disordered proteins. NMR titration of Arixtra into 15N-labeled tau R2* induced large chemical shift perturbations (CSPs) in 275VQIINK280 and downstream residues K281-D283, in which L282 and I278 displayed the largest shifts. NMR titration of Arixtra into 15N-labeled tau PRR2* induced the largest CSPs for residue R209 followed by residues S210 and R211. Residue-based CSP fitting showed that tau PRR2*–Arixtra interaction had a much stronger binding affinity (0.37–0.67 mM) than that of tau R2*–Arixtra (1.90–5.12 mM) interaction. (3) Conclusions: Our results suggested that PRR2 is a crucial domain for tau–heparin and tau–HS interaction.
Biomass and β-Glucosidase Production by the Cyanobacterium Pseudanabaena sp. under Heterotrophic Conditions
Authors: Karageorgou Dimitra , Sainis Ioannis , Touka Anastasia , Vareli Katerina , Stamatis Haralambos , Katapodis Petros
Published: October 2022

A cyanobacterium producing β-glucosidase was isolated from Lake Pamvotis located in Ioannina in Greece. This microorganism, named Pamv7, was identified as Pseudanabaena sp. using phylogenetic characterization. The high-throughput BiologMicroPlate™ method, used for the rapid assessment of heterotrophic potential, indicates that Pseudanabaena sp. metabolizes a wide range of organic substrates such as amino acids, carbohydrates, and carboxylic acids. When the strain grows in a culture medium containing cellobiose as a carbon source, it produces a significant amount of intracellular β-glucosidase. The effect of cellobiose concentration, nitrogen source, and nitrogen concentration of the growth medium, as well as the temperature of the culture, on biomass and β-glucosidase by Pseudanabaena sp., was studied. Biomass and β-glucosidase production by the strain in a lab-scale bioreactor at optimal conditions (10 g/L cellobiose, 1.5 g/L yeast, and 23 ± 1 °C) reached 2.8 g dry weight/L and 44 U/L, respectively. The protein and lipid content of the produced cyanobacterium biomass were 23% and 43 w/w, respectively. This study is the first report of β-glucosidase production by a cyanobacterial strain and concomitant high production of microalgae biomass, making Pseudanabaena sp. a promising microorganism in the field of enzyme biotechnology.
Phenotype and Neuronal Cytotoxic Function of Glioblastoma Extracellular Vesicles
Authors: Zhou Wenbo , Lovasz Daniel , Zizzo Zoë , He Qianbin , Coughlan Christina , Kowalski Robert , Kennedy Peter , Graner Arin , Lillehei Kevin , Ormond D. , Youssef A. , Graner Michael , Yu Xiaoli
Published: October 2022

Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. Extracellular vesicles (EVs) released by tumor cells play a critical role in cellular communication in the tumor microenvironment promoting tumor progression and invasion. We hypothesized that GBM EVs possess unique characteristics which exert effects on endogenous CNS cells including neurons, producing dose-dependent neuronal cytotoxicity. We purified EVs from the plasma of 20 GBM patients, 20 meningioma patients, and 21 healthy controls, and characterized EV phenotypes by electron microscopy, nanoparticle tracking analysis, protein concentration, and proteomics. We evaluated GBM EV functions by determining their cytotoxicity in primary neurons and the neuroblastoma cell line SH-SY5Y. In addition, we determined levels of IgG antibodies in the plasma in GBM (n = 82), MMA (n = 83), and controls (non-tumor CNS disorders and healthy donors, n = 50) with capture ELISA. We discovered that GBM plasma EVs are smaller in size and had no relationship between size and concentration. Importantly, GBM EVs purified from both plasma and tumor cell lines produced IgG-mediated, complement-dependent apoptosis and necrosis in primary human neurons, mouse brain slices, and neuroblastoma cells. The unique phenotype of GBM EVs may contribute to its neuronal cytotoxicity, providing insight into its role in tumor pathogenesis.
A Modular Chain Bioreactor Design for Fungal Productions
Authors: Kırdök Onur , Çetintaş Berker , Atay Asena , Kale İrem , Akyol Altun Tutku Didem , Hameş Elif Esin
Published: October 2022

Plastic bag bioreactors are single-use bioreactors, frequently used in solid culture fermentation. This study developed plastic bag bioreactors with more effective aeration conditions and particular connection elements that yield sensors, environmental control, and modular connectivity. This bioreactor system integrates the bags in a chain that circulates air and moisture through filtered connections. Within the present scope, this study also aimed to reveal that cultures in different plastic bags can be produced without affecting each other. In this direction, biomass production in the modular chain bioreactor (MCB) system developed in this study was compared to traditional bag systems. In addition, contamination experiments were carried out between the bags in the system, and it was observed that the filters in the developed system did not affect the microorganisms in different bags.