bluuebunny/crossref_metadata_2025 huggingface dataset:
Krill Oil and Its Bioactive Components as a Potential Therapy for Inflammatory Bowel Disease: Insights from In Vivo and In Vitro Studies
Authors: Liu Yingying , Robinson Ainsley M. , Su Xiao Qun , Nurgali Kulmira
Published: April 2024

Krill oil is extracted from krill, a small crustacean in the Antarctic Ocean. It has received growing attention because of krill oil’s unique properties and diverse health benefits. Recent experimental and clinical studies suggest that it has potential therapeutic benefits in preventing the development of a range of chronic conditions, including inflammatory bowel disease (IBD). Krill oil is enriched with long-chain n-3 polyunsaturated fatty acids, especially eicosapentaenoic and docosahexaenoic acids, and the potent antioxidant astaxanthin, contributing to its therapeutic properties. The possible underlying mechanisms of krill oil’s health benefits include anti-inflammatory and antioxidant actions, maintaining intestinal barrier functions, and modulating gut microbiota. This review aims to provide an overview of the beneficial effects of krill oil and its bioactive components on intestinal inflammation and to discuss the findings on the molecular mechanisms associated with the role of krill oil in IBD prevention and treatment.
A Flavonoid Glycoside Compound from Siraitia grosvenorii with Anti-Inflammatory and Hepatoprotective Effects In Vitro
Authors: Wu Juanjiang , Huang Huaxue , Gong Limin , Tian Xing , Peng Zhi , Zhu Yizhun , Wang Wei
Published: April 2024

Inflammation is a pivotal factor in the development and advancement of conditions like NAFLD and asthma. Diet can affect several phases of inflammation and significantly influence multiple inflammatory disorders. Siraitia grosvenorii, a traditional Chinese edible and medicinal plant, is considered beneficial to health. Flavonoids can suppress inflammatory cytokines, which play a crucial role in regulating inflammation. In the present experiments, kaempferol 3-O-α-L-rhamnoside-7-O-β-D-xylosyl(1→2)-O-α-L-rhamnoside (SGPF) is a flavonoid glycoside that was first isolated from S. grosvenorii. A series of experimental investigations were carried out to investigate whether the flavonoid component has anti-inflammatory and hepatoprotective effects in this plant. The researchers showed that SGPF has a stronger modulation of protein expression in LPS-induced macrophages (MH-S) and OA-induced HepG2 cells. The drug was dose-dependent on cells, and in the TLR4/NF-κB/MyD88 pathway and Nrf2/HO-1 pathway, SGPF regulated all protein expression. SGPF has a clear anti-inflammatory and hepatoprotective function in inflammatory conditions.
Mitochondrial Dysfunction in Chronic Obstructive Pulmonary Disease: Unraveling the Molecular Nexus
Authors: Li Chin-Ling , Liu Jui-Fang , Liu Shih-Feng
Published: April 2024

Chronic obstructive pulmonary disease (COPD) is a prevalent and debilitating respiratory disorder characterized by persistent airflow limitation and chronic inflammation. In recent years, the role of mitochondrial dysfunction in COPD pathogenesis has emerged as a focal point of investigation. This review endeavors to unravel the molecular nexus between mitochondrial dysfunction and COPD, delving into the intricate interplay of oxidative stress, bioenergetic impairment, mitochondrial genetics, and downstream cellular consequences. Oxidative stress, a consequence of mitochondrial dysfunction, is explored as a driving force behind inflammation, exacerbating the intricate cascade of events leading to COPD progression. Bioenergetic impairment sheds light on the systemic consequences of mitochondrial dysfunction, impacting cellular functions and contributing to the overall energy imbalance observed in COPD patients. This review navigates through the genetic landscape, elucidating the role of mitochondrial DNA mutations, variations, and haplogroups in COPD susceptibility and severity. Cellular consequences, including apoptosis, autophagy, and cellular senescence, are examined, providing insights into the intricate mechanisms by which mitochondrial dysfunction influences COPD pathology. Therapeutic implications, spanning antioxidant strategies, mitochondria-targeted compounds, and lifestyle modifications, are discussed in the context of translational research. Important future directions include identifying novel biomarkers, advancing mitochondria-targeted therapies, and embracing patient-centric approaches to redefine COPD management. This abstract provides a comprehensive overview of our review, offering a roadmap for understanding and addressing the molecular nexus between mitochondrial dysfunction and COPD, with potential implications for precision medicine and improved patient outcomes.
Humanoid Head Camera Stabilization Using a Soft Robotic Neck and a Robust Fractional Order Controller
Authors: Muñoz Jorge , de Santos-Rico Raúl , Mena Lisbeth , Monje Concepción A.
Published: April 2024

In this paper, a new approach for head camera stabilization of a humanoid robot head is proposed, based on a bio-inspired soft neck. During walking, the sensors located on the humanoid’s head (cameras or inertial measurement units) show disturbances caused by the torso inclination changes inherent to this process. This is currently solved by a software correction of the measurement, or by a mechanical correction by motion cancellation. Instead, we propose a novel mechanical correction, based on strategies observed in different animals, by means of a soft neck, which is used to provide more natural and compliant head movements. Since the neck presents a complex kinematic model and nonlinear behavior due to its soft nature, the approach requires a robust control solution. Two different control approaches are addressed: a classical PID controller and a fractional order controller. For the validation of the control approaches, an extensive set of experiments is performed, including real movements of the humanoid, different head loading conditions or transient disturbances. The results show the superiority of the fractional order control approach, which provides higher robustness and performance.
A Novel DNA Synthesis Platform Design with High-Throughput Paralleled Addressability and High-Density Static Droplet Confinement
Authors: Yang Shijia , Wang Dayin , Zhao Zequan , Wang Ning , Yu Meng , Zhang Kaihuan , Luo Yuan , Zhao Jianlong
Published: April 2024

Using DNA as the next-generation medium for data storage offers unparalleled advantages in terms of data density, storage duration, and power consumption as compared to existing data storage technologies. To meet the high-speed data writing requirements in DNA data storage, this paper proposes a novel design for an ultra-high-density and high-throughput DNA synthesis platform. The presented design mainly leverages two functional modules: a dynamic random-access memory (DRAM)-like integrated circuit (IC) responsible for electrode addressing and voltage supply, and the static droplet array (SDA)-based microfluidic structure to eliminate any reaction species diffusion concern in electrochemical DNA synthesis. Through theoretical analysis and simulation studies, we validate the effective addressing of 10 million electrodes and stable, adjustable voltage supply by the integrated circuit. We also demonstrate a reaction unit size down to 3.16 × 3.16 μm2, equivalent to 10 million/cm2, that can rapidly and stably generate static droplets at each site, effectively constraining proton diffusion. Finally, we conducted a synthesis cycle experiment by incorporating fluorescent beacons on a microfabricated electrode array to examine the feasibility of our design.
Highly Sensitive and Linear Resonator-Based Biosensor for White Blood Cell Counting: Feasible Measurement Method and Intrinsic Mechanism Exploration
Authors: Wang Yi-Ke , Shi Bo-Wen , Zhao Jun-Ming , Wang Yan-Xiong , Jiang Yan-Feng , Yang Gang-Long , Gao Xiao-Dong , Qiang Tian
Published: April 2024

Since different quantities of white blood cells (WBCs) in solution possess an adaptive osmotic pressure of cells, the WBCs themselves and in solution have similar concentrations, resulting in them having similar dielectric properties. Therefore, a microwave sensor could have difficulty in sensing the quantity variation when WBCs are in solution. This paper presents a highly sensitive, linear permittivity-inspired microwave biosensor for WBCs, counting through the evaporation method. Such a measurement method is proposed to record measurements after the cell solution is dripped onto the chip and is completely evaporated naturally. The proposed biosensor consists of an air-bridged asymmetric differential inductor and a centrally located circular fork-finger capacitor fabricated on a GaAs substrate using integrated passive fabrication technology. It is optimized to feature a larger sensitive area and improved Q-factor, which increases the effective area of interaction between cells and the electromagnetic field and facilitates the detection of their changes in number. The sensing relies on the dielectric properties of the cells and the change in the dielectric constant for different concentrations, and the change in resonance properties, which mainly represents the frequency shift, corresponds to the macroscopic change in the concentration of the cells. The microwave biosensors are used to measure biological samples with concentrations ranging from 0.25 × 106 to 8 × 106 cells per mL in a temperature (26.00 ± 0.40 °C) and humidity (54.40 ± 3.90 RH%) environment. The measurement results show a high sensitivity of 25.06 Hz/cells·mL−1 with a highly linear response of r2 = 0.99748. In addition, a mathematical modeling of individual cells in suspension is performed to estimate the dielectric constant of individual cells and further explain the working mechanism of the proposed microwave biosensor.
Synthesis and Biological Activities of Some Metal Complexes of Peptides: A Review
Authors: Marinova Petja , Tamahkyarova Kristina
Published: April 2024

Peptides, both natural and synthetic, are well suited for a wide range of purposes and offer versatile applications in different fields such as biocatalysts, injectable hydrogels, tumor treatment, and drug delivery. The research of the better part of the cited papers was conducted using various database platforms such as MetalPDB. The rising prominence of therapeutic peptides encompasses anticancer, antiviral, antimicrobial, and anti-neurodegenerative properties. The metals Na, K, Mg, Ca, Fe, Mn, Co, Cu, Zn, and Mo are ten of the twenty elements that are considered essential for life. Crucial for understanding the biological role of metals is the exploration of metal-bound proteins and peptides. Aside from essential metals, there are other non-essential metals that also interact biologically, exhibiting either therapeutic or toxic effects. Irregularities in metal binding contribute to diseases like Alzheimer’s, neurodegenerative disorders, Wilson’s, and Menkes disease. Certain metal complexes have potential applications as radiopharmaceuticals. The examination of these complexes was achieved by preforming UV–Vis, IR, EPR, NMR spectroscopy, and X-ray analysis. This summary, although unable to cover all of the studies in the field, offers a review of the ongoing experimentation and is a basis for new ideas, as well as strategies to explore and gain knowledge from the extensive realm of peptide-chelated metals and biotechnologies.
Correction: Dreismickenbecker et al. Electroencephalography-Based Effects of Acute Alcohol Intake on the Pain Matrix. Brain Sci. 2023, 13, 1659
Authors: Dreismickenbecker Elias , Zinn Sebastian , Romero-Richter Mara , Kohlhaas Madeline , Fricker Lukas R. , Petzel-Witt Silvana , Walter Carmen , Kreuzer Matthias , Toennes Stefan W. , Anders Malte
Published: April 2024

This paper [...]
Psychological Traditionality and Modernity and Happiness: The Different Happiness Levels in Chinese Students
Authors: Xu Erjia , Liao Sihua , Hu Ping
Published: April 2024

The conceptualization of happiness varies across different cultures. In Chinese culture, happiness includes oneself and hinges on others. Chinese social development has influenced psychological traditionality (PT), psychological modernity (PM), and personal happiness. Our study recruited 450 participants to examine the different happiness levels in Chinese students with diverse PT and PM. The results indicate that individuals scoring higher in PT and PM reported higher life satisfaction. Moreover, individuals scoring higher in PT reported more positive emotions, fewer negative emotions, and greater social well-being, while those scoring higher in PM reported more negative emotions and greater relationship happiness. The happiness of Chinese students comprised individual, relational, and societal levels and happiness at different levels related to Chinese PT and PM. The present study may promote cross-cultural understanding and potentially inform interventions for individual happiness within positive psychology.
Protection and Utilization of Historical Sites Using Digital Twins
Authors: Liu Zherong , Wang Jiang
Published: April 2024

Historical sites are incomplete relics left by human activities and are also valuable resources for human society, with most of them buried deep underground. Because the protection of original historical sites is difficult, very few sites choose this method. Many funerary objects that have been in historical sites are scattered in museums, losing their original context and complicating their utilization. Thus, using digital technology to protect and utilize historical sites and to explore their outstanding value from historical, aesthetic, and anthropological perspectives is a foremost concern. Therefore, this study aims to develop a comprehensive method for the protection and utilization of historical sites, that is, digital protection and utilization based on a digital twin. We constructed a historical site digital twin model using qualitative and vertical methods, including a physical entity, virtual entity, twin data center, digital twin service, and connection. We also established a technical framework of data acquisition and processing, digital protection, and digital utilization, forming a layered management and application of digital resources. In digital protection, information in the real world and the virtual world are connected to monitor risks, collect data, create simulations, and propose protection strategies, quickly and accurately. In digital utilization, the knowledge graph is constructed to associate seemingly unrelated information, explore potential knowledge, and improve information sharing. In addition, the method is validated by means of case studies of historical sites in China. In this paper, the historical sites of the Northern Qi Dynasty in Taiyuan, Shanxi, especially the Xuxianxiu Tomb and Lourui Tomb, are discussed in detail. The results indicate that this method is effective for the protection and utilization of historical sites.
Implementation of Wood-Framed Buildings in the Nordic Region: A MADAMOS (an Integrated Multi-Criteria Decision-Making Approach for Profitable Realization Alternatives) Method
Authors: Ustinovichius Leonas , Turskis Zenonas , Miedziałowski Czeslaw , Vaišnoras Mantas
Published: April 2024

This study optimizes the sustainable implementation of light wood-framed buildings in the Nordic region using multi-criteria decision-making. Integrating prefabrication, transportation logistics, and multi-criteria decision-making (MCDM) methods enhances cost efficiency, time savings, and quality assurance. Significant international and local impact promotes sustainable construction practices. Strong promotion fosters industry-wide adoption. The presented framework enables stakeholders to make informed decisions, enhancing the efficiency and effectiveness of building implementation processes and fostering sustainable development in the construction industry.
Development of Eco-Friendly Soil Improvement Agent for Enhanced Slope Stability and Erosion Control in Urban Areas
Authors: Kang Dae-Hung , Kim Jaehong
Published: April 2024

Due to the impact of climate change, extreme rainfall events are becoming more frequent, resulting in shallow slope collapse and erosion that trigger debris flows. While traditional reinforcement methods like anchoring and nailing are effective, they can be costly and environmentally unfriendly. To address this issue, researchers have investigated using in situ soil reinforcement with vegetation, which is a more sustainable and economical option. In this study, a soil improvement agent was developed using leaf mold and herbal medicine to promote vegetation growth. Adding microcement and gypsum hemihydrate increased the shear strength of the soil, preventing surface erosion. A laboratory test confirmed that the combination of these ingredients effectively increased the soil’s resistance to erosion caused by rainfall. The soil improvement agent proposed in this study was applied to the case of the slope failure in the Gwangju area, South Korea, to confirm the slope stability for 10 days of rainfall. The results of numerical analysis confirmed that the reinforced slope cured by the pozzolanic reaction using the developed material improved the slope stability by 36% compared to the original soil slope during the rainy season.
The Impact of Indoor Air Humidity on the Infiltration of Ambient Particles
Authors: Qiu Jiayi , Zhang Haixi , Liu Jialu , Liu Yanhua
Published: April 2024

Ambient particles contribute to occupant exposure as they infiltrate indoor environments through building envelope cracks, impacting indoor air quality. This study investigates the impact of indoor air humidity on the infiltration of ambient particles, highlighting humidity’s crucial role in influencing particle dynamics in indoor environments. Employing a controlled chamber system, we conducted experiments to quantify the infiltration of size-resolved particles under varying relative humidity (RH) conditions. Both the total number and the mass concentration of particles increased with RH in the experimental chamber. The smallest particles (0.3–0.4 μm) experienced reduced infiltration at higher RH levels due to hygroscopic growth, while intermediate-sized particles showed increased infiltration, resulted from coagulation effects. Large particles (>1.0 μm) demonstrated reduced infiltration factors, caused by lower penetration and higher deposition rates, with minimal impact from RH changes. Our findings reveal that RH influences particle hygroscopic growth, deposition rate, and coagulation process, thereby affecting indoor particle size distribution and concentration.
Experimental Study on Anti-Aging Effect of Asphalt Binder Liquid Anti-Aging Agent
Authors: Fu Chunping , Wang Zhen , Song Shanglin , Yao Xiupeng , Wang Fangliang , Wei Lewen , Guo Meng
Published: April 2024

During its service life, asphalt pavement is affected by environmental factors such as heat, light, oxygen and moisture. The asphalt binder ages, resulting in a decrease in the performance of the asphalt pavement. Therefore, the development and application of an asphalt anti-aging agent is of great significance. In this paper, the road performance of the developed asphalt binder liquid anti-aging agent was verified in the two scales of asphalt binder and asphalt mixture, respectively. Firstly, a simple aging test method was proposed using needle penetration as the evaluation index. The aging time of the simple aging method was determined to be 20 h. Secondly, the addition of anti-aging agents improved the low-temperature performance of the asphalt binder and had a less adverse effect on the high-temperature performance. The test values of needle penetration and the ductility of the asphalt binder increased and the values of the softening point decreased. And the road performance of the anti-aging asphalt mixture basically met the specification requirements. The addition of the anti-aging agent improved the low-temperature performance of the aged asphalt mixture by 16%, which is of great significance for improving the service life of asphalt pavement.
Structural Behavior of Precast Monolithic Composite Beams with ECC Prefabricated Shells
Authors: Lu Tingting , Li Zhilong , Pan Jiaojiao , Guan Kai
Published: April 2024

Engineered Cementitious Composite (ECC) was used in the prefabricated shell of a precast composite beam, and the validity of the ECC in the precast monolithic composite beam’s flexural performance was studied. Flexural performance tests were conducted on six specimens to analyze their failure state, failure mechanism, bearing capacity, and deformation capacity. Compared with the Reinforced Concrete (RC) prefabricated shell composite beam, the ECC prefabricated shell composite beam showed a higher bearing capacity and lower damage degree. The yielding load of specimen PSMCB−1 increased by 5.6%. The maximum load of specimen PSMCB−1 reached 256.6 kN, having increased by 8.1%. When the tests stopped, the maximum crack width of specimen PSMCB−1 was 4.1mm, while the maximum crack width of specimen PSMCB−6 reached 9.0 mm. The ECC material in the prefabricated shell also delayed the yielding of the steel bars in the shell.
Design Strategies to Improve Metro Transit Station Walking Environments: Five Stations in Chongqing, China
Authors: Yao Chungui , Li Gaoyuan , Yan Shuiyu
Published: April 2024

While transit-oriented development (TOD) has been widely adopted in urban design alongside the expansion of urban metro transit, the creation of pedestrian-friendly environments has often been overlooked during implementation. This has resulted in a lower walking advantage around metro transit stations. To address this issue and encourage walking and public transport use in metro transit station areas, this study undertook a quantitative comparative analysis of the pedestrian environment in five Chongqing metro transit station areas. The analysis focused on three key dimensions: “comprehensive evaluation”, “basic scale”, and “structural quality”. The comprehensive evaluation considered factors such as the pedestrian catchment area ratio, POI kernel density distribution, and crowd agglomeration. The basic scale dimension comprised floor area ratio, building density, pedestrian road density, and the quantity of station entrances and exits. Finally, structural quality factors included land use type mixing degree, POI function mixing degree, intersection connectivity, median street length, pedestrian route directness, and green view index. Based on these analyses, this study proposes a series of pedestrian environment design strategies including land use and transportation. The strategies for land use advocate for “developing compact and diverse land use”, “strengthening attraction of station center”, “positioning large projects on the edge”, “restricting private transportation capabilities”. The strategies for transportation consist of “increasing pedestrian road density”, “traffic calming organization”, “subdivision of road types”, and “three-dimensional pedestrian traffic system”. These strategies aim to create a more humanized and environmentally friendly pedestrian environment, proactively rise to the challenge of climate change, thereby cultivating sustainable urban development.
An Improved Trilateral Localization Technique Fusing Extended Kalman Filter for Mobile Construction Robot
Authors: Zeng Lingdong , Guo Shuai , Zhu Mengmeng , Duan Hao , Bai Jie
Published: April 2024

Semi-open and chaotic environments of building sites are considered primary challenges for the localization of mobile construction robots. To mitigate environmental limitations, an improved trilateral localization technique based on artificial landmarks fusing the extended Kalman filters (EKFs) is proposed in this paper. The reflective intensity of the onboard laser is employed to identify artificial landmarks arranged in the ongoing construction environment. A trilateral positioning algorithm is then adopted and evaluated based on artificial landmarks. Multi-sensor fusion, combined with the EKF, is included to improve the positioning accuracy and reliability of the robot in complex conditions. We constructed validation scenarios in the Gazebo simulation environment to verify the required localization functionality. Concurrently, we established simulated testing environments in real-world settings, where the practicality of the proposed technique was validated through the fitting of ideal and actual localization trajectories. The effectiveness of the proposed technique was corroborated through comparative experimental results.
Association between Hometown Landholdings and Housing Quality of Rural Migrants in Urban Areas: Evidence from China
Authors: Wang Wei , Luo Gai , Gong Xinzhi
Published: April 2024

Urbanization progress in developing countries is reflected in the urban living conditions of their rural migrants. Housing quality, in particular, is linked to migrants’ social integration and development of human capital. In China, where urban housing is highly stratified by urban citizenship via the “hukou” or household registration system, improving housing quality is a top priority for the central government in its pursuit of human-centered urbanization. Despite some social and economic elements affecting migrants’ housing quality being documented, few studies have analyzed the determinants of rural migrants’ housing quality from the perspective of rural landholdings or possession of use rights of rural lands, which is endowed by the land system of China. Using large micro-data from the China Migrant Dynamic Survey (CMDS), this study investigates the association between hometown landholdings and rural migrants’ housing quality in their host cities. The empirical results suggest that possessing rural landholdings in their hometown is negatively correlated with rural migrants’ housing quality in their host cities, wherein rural migrants’ hukou transfer intention is found as an intermediary factor. Furthermore, the heterogeneity of the relationship across demographic characteristics and regions was investigated. In addition, short-term revenue derived from hometown landholdings is also verified to have a very limited positive effect on migrants’ housing quality. Land transfer policies customized for subgroups of rural migrants across sociological attributes and urban stratification are concluded finally.
Seismic Performance Evaluation of a Chilean RC Building Damaged during the Mw8.8 Chile Earthquake
Authors: Suquillo Betzabeth , Rojas Fabián , Massone Leonardo M.
Published: April 2024

Chile, recognized as one of the world’s most earthquake-prone nations, has gained valuable insights from significant earthquakes, such as those in 1985 and 2010, which have influenced updates to the nation’s design codes. Although Chile’s seismic design approach has been largely effective in recent earthquakes and demonstrated an “operational” performance level in most structures, performance-based design (PBD) methods have not yet been officially incorporated as valid approaches in the Chilean seismic design codes for buildings. However, in 2017, the Chilean Association on Seismology and Earthquake Engineering (ACHISINA) introduced a PBD approach, primarily for verification purposes, based on the Los Angeles Tall Buildings Structural Design Council (LATBSDC) framework. In this work, firstly, we provide an overview of Chile’s PBD methodology, focusing on the thresholds for various performance levels. These levels are established through experimental and numerical analysis, correlating performance with permissible damage levels. The second part of the paper examines the seismic performance of a residential building, designed before the 2010 Maule earthquake and subsequently damaged, using Chile’s PBD guidelines. This case study highlights the implementation and effectiveness of PBD for assessing seismic resilience in Chilean structures.
Liquid Sloshing in Soil-Supported Multiple Cylindrical Tanks Equipped with Baffle under Horizontal Excitation
Authors: Sun Ying , Meng Xun , Zhang Zhong , Gu Zhenyuan , Wang Jiadong , Zhou Ding
Published: April 2024

The dynamic behavior of liquid storage tanks is one of the research issues about fluid–structure interaction problems. The analysis errors of the dynamics of multiple adjacent tanks can exist if neglecting soil–tank interaction since tanks are typically supported on flexible soil. In the present paper, the dynamics of a group of baffled cylindrical storage tanks supported on a circular surface foundation and undergoing horizontal excitation are analytically examined. For upper multiple tank–liquid–baffle subsystems, accurate solutions to the velocity potential for liquid sloshing are acquired according to the subdomain partition technique. A theoretical model is utilized to portray the continuous sloshing of each tank. For the soil–foundation subsystem, a lumped-parameter model is used to characterize the impacts of soil on upper-tank structures using Chebyshev complex polynomials that present the fitting results of horizontal, rocking, and coupling impedance functions. Then, a model of the soil–foundation–tank–liquid–baffle system is constructed on the basis of the substructure approach. The present sloshing frequencies, sloshing height, and hydrodynamic shear as well as the moment under rigid/soft soil foundations are compared to the available exact results and the numerical results to prove the validity of the present model. The error of the maximum sloshing height between the present and the numerical solutions is within 5.27%; the solution efficiency of system dynamics from the present model is 40–50 times faster than that from the ADINA model. A detailed parameter analysis of the dynamic characteristics and earthquake responses of the coupling system is presented. The research novelty is that an equivalent analytical model is presented, and it allows for investigating the dynamics of soil-supported multiple cylindrical tanks with a baffle, providing acceptable accuracy and high calculation efficiency.
A Parametric Study Investigating the Dowel Bar Load Transfer Efficiency in Jointed Plain Concrete Pavement Using a Finite Element Model
Authors: Yaqoob Saima , Silfwerbrand Johan , Balieu Romain Gabriel Roger
Published: April 2024

Transverse joints are introduced in jointed plain concrete pavement systems to mitigate the risk of cracks that can develop due to shrinkage and temperature variations. However, the structural behaviour of jointed plain concrete pavement (JPCP) is significantly affected by the transverse joint, as it creates a discontinuity between adjacent slabs. The performance of JPCP at the transverse joints is enhanced by providing steel dowel bars in the traffic direction. The dowel bar provides reliable transfer of traffic loads from the loaded side of the joint to the unloaded side, known as load transfer efficiency (LTE) or joint efficiency (JE). Furthermore, dowel bars contribute to the slab’s alignment in the JPCP. Joints are the critical component of concrete pavements that can lead to various distresses, necessitating rehabilitation. The Swedish Transport Administration (Trafikverket) is concerned with the repair of concrete pavement. Precast concrete slabs are efficient for repairing concrete pavement, but their performance relies on well-functioning dowel bars. In this study, a three-dimensional finite element model (3D-FEM) was developed using the ABAQUS software to evaluate the structural response of JPCP and analyse the flexural stress concentration in the concrete slab by considering the dowel bar at three different locations (i.e., at the concrete slabs’ top, bottom, and mid-height). Furthermore, the structural response of JPCP was also investigated for several important parameters, such as the joint opening between adjacent slabs, mispositioning of dowel bars (horizontal, vertical, and longitudinal translations), size (diameter) of the dowel bar, and bond between the slab and the dowel bar. The study found that the maximum LTE occurred when the dowel bar was positioned at the mid-depth of the concrete slab. An increase in the dowel bar diameter yielded a 3% increase in LTE. Conversely, the increase in the joint opening between slabs led to a 2.1% decrease in LTE. Additionally, the mispositioning of dowel bars in the horizontal and longitudinal directions showed a 2.1% difference in the LTE. However, a 0.5% reduction in the LTE was observed for a vertical translation. Moreover, an approximately 0.5% increase in LTE was observed when there was improved bonding between the concrete slab and dowel bar. These findings can be valuable in designing and evaluating dowel-jointed plain concrete pavements.
Prediction of Torque Capacity in Circular Concrete-Filled Double-Skin Tubular Members under Pure Torsion via Machine Learning and Shapley Additive Explanations Interpretation
Authors: Simwanda Lenganji , Ikotun Bolanle Deborah
Published: April 2024

The prediction of torque capacity in circular Concrete-Filled Double-Skin Tubular (CFDST) members under pure torsion is considered vital for structural design and analysis. In this study, torque capacity is predicted using machine learning (ML) algorithms, such as Categorical Boosting (CatBoost), Extreme Gradient Boosting (XGBoost), Gradient Boosting Machine (GBM), Random Forest (RF), and Decision Tree (DT), which are employed. The interpretation of the results is conducted using Shapley Additive Explanations (SHAPs). The performance of these ML models is evaluated against two traditional analytical formulas that have been proposed and are available in the literature. Through comprehensive analysis, it is shown that superior predictive capabilities are possessed by the CatBoost and XGBoost models, characterized by high R2 values and minimal mean errors. Additionally, insights into the influence of input features are provided by SHAP interpretation, with an emphasis on key parameters such as concrete compressive strength and steel tube dimensions. The gap between empirical models and ML techniques is bridged by this study, offering engineers a more accurate and efficient tool for CFDST structural design. Significant implications for optimizing CFDST column designs and advancing structural engineering practices are presented by these findings. Directions for future research include the further refinement of ML models and the integration of probabilistic analyses for enhanced structural resilience. Overall, the transformative potential of ML and SHAP interpretation in advancing the field of structural engineering is showcased by this study.
The Micro-Immunotherapy Medicine 2LPAPI® Displays Immune-Modulatory Effects in a Model of Human Papillomavirus Type-16 L1-Protein Capsid-Treated Human Peripheral Blood Mononuclear Cells and Antiproliferative Effects in a Model of Cervical Cancer Cells
Authors: Jacques Camille , Marchand Flora , Chatelais Mathias , Albinet Virginie , Coustal Claire , Floris Ilaria
Published: April 2024

Human papillomavirus (HPV) is the second most common infectious agent causing cancer. Persistent infection with high-risk (HR)-HPV can lead to cervical intra-epithelial neoplasia and cervical carcinomas (CC). While host immune response is necessary for viral clearance, chronic immune activation contributes to a low-grade inflammation that can ultimately lead to carcinogenesis. The micro-immunotherapy medicine (MIM) 2LPAPI® could be a valuable tool to manage the clearance of the virus and reduce the risk of developing CC. In this in vitro study, we aimed to investigate its mode of action. We showed that actives from the MIM increased the IL-6, IFN-γ, and IP-10 secretion in human peripheral blood mononuclear cells (PBMCs) exposed to peptides derived from the HPV-16 capsid (HPV16(L1)). This could reflect an increase in the immune activity toward HPV-16. At the same time, some active substances reduced the lympho-proliferation and the expression of T-cell activation markers. Finally, some of the MIM actives displayed antiproliferative effects in CC-derived HeLa cells under serum-starvation conditions. Altogether, this body of data highlighted for the first time the dual effect of MIM in the framework of HR-HPV infections as a potential (i) immune modulator of HPV16(L1)-treated PBMCs and (ii) antiproliferative agent of HPV-positive CC cells.
Prognostic Indicators of Overall Survival in Hepatocellular Carcinoma Patients Undergoing Liver Resection
Authors: Ursu Cristina-Paula , Ciocan Andra , Ursu Ștefan , Ciocan Răzvan Alexandru , Gherman Claudia Diana , Cordoș Ariana-Anamaria , Vălean Dan , Pop Rodica Sorina , Furcea Luminița Elena , Procopeț Bogdan , Ștefănescu Horia , Moiș Emil Ioan , Al Hajjar Nadim , Graur Florin
Published: April 2024

Hepatocellular carcinoma (HCC) is the predominant form of primary liver cancer and the third contributor to malignancy-related deaths worldwide. The hepatic venous pressure gradient (HVPG), transient elastography-liver stiffness measurement (TE-LSM), and the association between TBS (tumor burden score), alpha-fetoprotein levels, and the Child–Pugh classification (TAC score) can serve as valuable prognostic indicators for these patients. Therefore, the main objective of our research was to analyze the prognostic value of the HVPG, TE-LSM, TBS, and TAC scores. An observational and survival study was conducted on 144 subjects. Our findings indicated that HVPG greater than 10 mmHg, AFP surpassing 400 ng/mL, an advanced C–P class, and low TAC score are independent predictors of overall survival. During the multivariate analysis, AFP serum levels and C–P class proved statistically significant. The present study revealed significant differences in overall survival between the two groups divided upon HVPG values and settled by the cutoff of 10 mmHg (p = 0.02). Moreover, by dividing the cohort into three groups based on the TAC score (very low, low, and moderate), statistically significant differences in overall survival were observed across the groups (p = 0.004).
PAX1/JAM3 Methylation and HPV Viral Load in Women with Persistent HPV Infection
Authors: Li Mingzhu , Zhao Chao , Zhang Xiaobo , Li Jingran , Zhao Yun , Zhang Wei , Ren Lihua , Wei Lihui
Published: April 2024

The relationship of PAX1/JAM3 methylation as well as HPV viral load (VL) with cervical lesions has been reported, but their role in persistent HPV infection without cervical high-grade lesions has not been fully elucidated. A total of 231 females diagnosed with persistent HPV infection and pathologically confirmed absence of high-grade cervical lesions were selected from the Colposcopy Outpatient Clinic of Peking University People’s Hospital, from March 2023 to December 2023. They were categorized into two groups based on the duration of HPV infection: the HPV persistent less than 3 years group and the more than 3 years group. PAX1/JAM3 methylation and HPV VL were determined by real-time PCR and BioPerfectus Multiplex Real-Time (BMRT)-HPV reports type-specific VL/10,000 cells, respectively. The average age of individuals with HPV infection lasting more than 3 years was higher compared to those with less than 3 years (48.9 vs. 45.1 years), with a statistically significant difference. Among the participants, 81.8% (189/231) had no previous screening. The methylation levels of JAM3 and PAX1 were significantly higher in individuals with HPV infection persisting for more than 3 years compared to those with less than 3 years, with a statistically significant difference (p < 0.05). There was a significant correlation between PAX1 and JAM3 methylation (p < 0.001), which could be used as cumulative evidence of HPV infection duration before the occurrence of precancerous lesions. The incidence of vaginal intraepithelial lesions was higher in individuals with HPV infection persisting for more than 3 years compared to those with less than 3 years, and HPV VL can be used as an indicative biomarker for concurrent cervical–vaginal lesions, especially for HPV other than 16/18 genotypes.
A Plausible Framework Reveals Potential Similarities in the Regulation of Immunity against Some Cancers and Some Infectious Agents: Implications for Prevention and Treatment
Authors: Bretscher Peter A.
Published: April 2024

Different frameworks, which are currently employed to understand how immune responses are regulated, can account for different observations reported in the classical literature. I have argued that the predominant frameworks, employed over the last two/three decades to analyze the circumstances that determine whether an immune response is generated or this potential is ablated, and that determine the class of immunity an antigen induces, are inconsistent with diverse classical observations. These observations are “paradoxical” within the context of these frameworks and, consequently, tend to be ignored by most contemporary researchers. One such observation is that low and high doses of diverse types of antigen result, respectively, in cell-mediated and IgG antibody responses. I suggest these paradoxes render these frameworks implausible. An alternative framework, The Threshold Hypothesis, accounts for the paradoxical observations. Some frameworks are judged more plausible when found to be valuable in understanding findings in fields beyond their original compass. I explore here how the Threshold Hypothesis, initially based on studies with chemically well-defined and “simple antigens”, most often a purified protein, can nevertheless shed light on diverse classical and more recent observations in the fields of immunity against cancer and against infectious agents, thus revealing common, immune mechanisms. Most cancers and some pathogens are best contained by cell-mediated immunity. The success of the Threshold Hypothesis has encouraged me to employ it as a basis for proposing strategies to prevent and to treat cancer and those infectious diseases caused by pathogens best contained by a cell-mediated attack.
Correction: Garza-Morales et al. Temozolomide Enhances Triple-Negative Breast Cancer Virotherapy In Vitro. Cancers 2018, 10, 144
Authors: Garza-Morales Rodolfo , Gonzalez-Ramos Roxana , Chiba Akiko , Montes de Oca-Luna Roberto , McNally Lacey R. , McMasters Kelly M. , Gomez-Gutierrez Jorge G.
Published: April 2024

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Translational Aspects in Metaplastic Breast Carcinoma
Authors: Barrientos-Toro Elizve Nairoby , Ding Qingqing , Raso Maria Gabriela
Published: April 2024

Breast cancer is the most common cancer among women. Metaplastic breast carcinoma (MpBC) is a rare, heterogeneous group of invasive breast carcinomas, which are classified as predominantly triple-negative breast carcinomas (TNBCs; HR-negative/HER2-negative). Histologically, MpBC is classified into six subtypes. Two of these are considered low-grade and the others are high-grade. MpBCs seem to be more aggressive, less responsive to neoadjuvant chemotherapy, and have higher rates of chemoresistance than other TNBCs. MpBCs have a lower survival rate than expected for TNBCs. MpBC treatment represents a challenge, leading to a thorough exploration of the tumor immune microenvironment, which has recently opened the possibility of new therapeutic strategies. The epithelial–mesenchymal transition in MpBC is characterized by the loss of intercellular adhesion, downregulation of epithelial markers, underexpression of genes with biological epithelial functions, upregulation of mesenchymal markers, overexpression of genes with biological mesenchymal functions, acquisition of fibroblast-like (spindle) morphology, cytoskeleton reorganization, increased motility, invasiveness, and metastatic capabilities. This article reviews and summarizes the current knowledge and translational aspects of MpBC.
Exploring The Prognostic Significance of SET-Domain Containing 2 (SETD2) Expression in Advanced and Castrate-Resistant Prostate Cancer
Authors: Gamallat Yaser , Felipe Lima Joema , Seyedi Sima , Li Qiaowang , Rokne Jon George , Alhajj Reda , Ghosh Sunita , Bismar Tarek A.
Published: April 2024

SET-domain containing 2 (SETD2) is a histone methyltransferase and an epigenetic modifier with oncogenic functionality. In the current study, we investigated the potential prognostic role of SETD2 in prostate cancer. A cohort of 202 patients’ samples was assembled on tissue microarrays (TMAs) containing incidental, advanced, and castrate-resistant CRPCa cases. Our data showed significant elevated SETD2 expression in advanced and castrate-resistant disease (CRPCa) compared to incidental cases (2.53 ± 0.58 and 2.21 ± 0.63 vs. 1.9 ± 0.68; p < 0.001, respectively). Interestingly, the mean intensity of SETD2 expression in deceased vs. alive patients was also significantly different (2.31 ± 0.66 vs. 2 ± 0.68; p = 0.003, respectively). Overall, high SETD2 expression was found to be considered high risk and was significantly associated with poor prognosis and worse overall survival (OS) (HR 1.80; 95% CI: 1.28–2.53, p = 0.001) and lower cause specific survival (CSS) (HR 3.14; 95% CI: 1.94–5.08, p < 0.0001). Moreover, combining high-intensity SETD2 with PTEN loss resulted in lower OS (HR 2.12; 95% CI: 1.22–3.69, p = 0.008) and unfavorable CSS (HR 3.74; 95% CI: 1.67–8.34, p = 0.001). Additionally, high SETD2 intensity with ERG positive expression showed worse prognosis for both OS (HR 1.99, 95% CI 0.87–4.59; p = 0.015) and CSS (HR 2.14, 95% CI 0.98–4.68, p = 0.058). We also investigated the protein expression database TCPA, and our results showed that high SETD2 expression is associated with a poor prognosis. Finally, we performed TCGA PRAD gene set enrichment analysis (GSEA) data for SETD2 overexpression, and our data revealed a potential association with pathways involved in tumor progression such as the AMPK signaling pathway, the cAMP signaling pathway, and the PI3K-Akt signaling pathway, which are potentially associated with tumor progression, chemoresistance, and a poor prognosis.
Simultaneous Visualization of Lung Tumor and Intersegmental Plane during Pulmonary Segmentectomy by Intravenous Injection of Indocyanine Green
Authors: Kim Kyungsu , Jeon Ok Hwa , Choi Byeong Hyeon , Rho Jiyun , Lee Jun Hee , Eo Jae Seon , Kim Beop-Min , Kim Hyun Koo
Published: April 2024

Segmentectomy is a targeted surgical approach tailored for patients with compromised health and early-stage lung cancer. The key to successful segmentectomy lies in precisely identifying the tumor and intersegmental planes to ensure adequate resection margins. In this study, we aimed to enhance this process by simultaneously visualizing the tumor and intersegmental planes through the intravenous injection of indocyanine green (ICG) at different time points and doses. Lung tumors were detected by intravenous injection of ICG at a dose of 2 mg/kg 12 h before surgery in a rabbit model. Following the dissection of the pulmonary artery, vein, and bronchi of the target segment, 0.6 mg/kg of ICG was injected intravenously to detect the intersegmental plan. Fluorescent images of the lung tumors and segments were acquired, and the fluorescent signal was quantified using the signal-to-background ratio (SBR). Finally, a pilot study of this method was conducted in three patients with lung cancer. In a preclinical study, the SBR of the tumor (4.4 ± 0.1) and nontargeted segments (10.5 ± 0.8) were significantly higher than that of the targeted segment (1.6 ± 0.2) (targeted segment vs. nontarget segment, p < 0.0001; target segment vs. tumor, p < 0.01). Consistent with preclinical results, lung tumors and the intersegmental plane were successfully detected in patients with lung cancer. Consequently, adequate resection margins were identified during the surgery, and segmentectomy was successfully performed in patients with lung cancer. This study is the first to use intravenous ICG injections at different time points and doses to simultaneously detect lung cancer and intersegmental planes, thereby achieving segmentectomy for lung cancer.