bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydroxyl-Decorated Diiron Complex as a [FeFe]-Hydrogenase Active Site Model Complex: Light-Driven Photocatalytic Activity and Heterogenization on Ethylene-Bridged Periodic Mesoporous Organosilica
Authors: Amaro-Gahete Juan , Esquivel Dolores , Pavliuk Mariia V. , Jiménez-Sanchidrián César , Tian Haining , Ott Sascha , Romero-Salguero Francisco J.
Published: February 2022

A biomimetic model complex of the [FeFe]-hydrogenase active site (FeFeOH) with an ethylene bridge and a pendant hydroxyl group has been synthesized, characterized and evaluated as catalyst for the light-driven hydrogen production. The interaction of the hydroxyl group present in the complex with 3-isocyanopropyltriethoxysilane provided a carbamate triethoxysilane bearing a diiron dithiolate complex (NCOFeFe), thus becoming a potentially promising candidate for anchoring on heterogeneous supports. As a proof of concept, the NCOFeFe precursor was anchored by a grafting procedure into a periodic mesoporous organosilica with ethane bridges (EthanePMO@NCOFeFe). Both molecular and heterogenized complexes were tested as catalysts for light-driven hydrogen generation in aqueous solutions. The photocatalytic conditions were optimized for the homogenous complex by varying the reaction time, pH, amount of the catalyst or photosensitizer, photon flux, and the type of light source (light-emitting diode (LED) and Xe lamp). It was shown that the molecular FeFeOH diiron complex achieved a decent turnover number (TON) of 70 after 6 h, while NCOFeFe and EthanePMO@NCOFeFe had slightly lower activities showing TONs of 37 and 5 at 6 h, respectively.
Altered RBP1 Gene Expression Impacts Epithelial Cell Retinoic Acid, Proliferation, and Microenvironment
Authors: Yu Jianshi , Perri Mariarita , Jones Jace W. , Pierzchalski Keely , Ceaicovscaia Natalia , Cione Erika , Kane Maureen A.
Published: February 2022

Vitamin A is an essential diet-derived nutrient that has biological activity affected through an active metabolite, all-trans retinoic acid (atRA). Retinol-binding protein type 1 (RBP1) is an intracellular chaperone that binds retinol and retinal with high affinity, protects retinoids from non-specific oxidation, and delivers retinoids to specific enzymes to facilitate biosynthesis of RA. RBP1 expression is reduced in many of the most prevalent cancers, including breast cancer. Here, we sought to understand the relationship between RBP1 expression and atRA biosynthesis in mammary epithelial cells, as well as RBP1 expression and atRA levels in human mammary tissue. We additionally aimed to investigate the impact of RBP1 expression and atRA on the microenvironment as well as the potential for therapeutic restoration of RBP1 expression and endogenous atRA production. Using human mammary ductal carcinoma samples and a series of mammary epithelial cell lines representing different stages of tumorigenesis, we investigated the relationship between RBP1 expression as determined by QPCR and atRA via direct liquid chromatography-multistage-tandem mass spectrometry-based quantification. The functional effect of RBP1 expression and atRA in epithelial cells was investigated via the expression of direct atRA targets using QPCR, proliferation using Ki-67 staining, and collagen deposition via picrosirius red staining. We also investigated the atRA content of stromal cells co-cultured with normal and tumorigenic epithelial cells. Results show that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Knock down of RBP1 expression using shRNA or overexpression of RBP1 supported a direct relationship between RBP1 expression with atRA. Increases in cellular atRA were able to activate atRA direct targets, inhibit proliferation and inhibit collagen deposition in epithelial cell lines. Conditions encountered in tumor microenvironments, including low glucose and hypoxia, were able to reduce RBP1 expression and atRA. Treatment with either RARα agonist AM580 or demethylating agent Decitabine were able to increase RBP1 expression and atRA. Cellular content of neighboring fibroblasts correlated with the RA producing capacity of epithelial cells in co-culture. This work establishes a direct relationship between RBP1 expression and atRA, which is maintained when RBP1 expression is restored therapeutically. The results demonstrate diseases with reduced RBP1 could potentially benefit from therapeutics that restore RBP1 expression and endogenous atRA.
Enhanced Wild-Type MET Receptor Levels in Mouse Hepatocytes Attenuates Insulin-Mediated Signaling
Authors: Rada Patricia , Lamballe Fabienne , Carceller-López Elena , Hitos Ana B. , Sequera Celia , Maina Flavio , Valverde Ángela M.
Published: February 2022

Compelling evidence points to the MET receptor tyrosine kinase as a key player during liver development and regeneration. Recently, a role of MET in the pathophysiology of insulin resistance and obesity is emerging. Herein, we aimed to determine whether MET regulates hepatic insulin sensitivity. To achieve this, mice in which the expression of wild-type MET in hepatocytes is slightly enhanced above endogenous levels (Alb-R26Met mice) were analyzed to document glucose homeostasis, energy balance, and insulin signaling in hepatocytes. We found that Alb-R26Met mice exhibited higher body weight and food intake when compared to R26stopMet control mice. Metabolic analyses revealed that Alb-R26Met mice presented age-related glucose and pyruvate intolerance in comparison to R26stopMet controls. Additionally, in Alb-R26Met mice, high MET levels decreased insulin-induced insulin receptor (IR) and AKT phosphorylation compared to control mice. These results were corroborated in vitro by analyzing IR and AKT phosphorylation in primary mouse hepatocytes from Alb-R26Met and R26stopMet mice upon insulin stimulation. Moreover, co-immunoprecipitation assays revealed MET-IR interaction under both basal and insulin stimulation conditions; this effect was enhanced in Alb-R26Met hepatocytes. Altogether, our results indicate that enhanced MET levels alter hepatic glucose homeostasis, which can be an early event for subsequent liver pathologies.
Vaping in Asthmatic Adolescents: Time to Deal with the Elephant in the Room
Authors: Chatziparasidis Grigorios , Kantar Ahmad
Published: February 2022

Adolescence is a period characterized by developmental, psychological, and psychosocial alterations, with a major impact on youths’ attitudes and perceptions. Adolescents with asthma may not comply with treatment and may develop risky behaviors, including smoking, vaping, and other substance use, leading to unanticipated exacerbations and consequences. Vaping has become extremely popular in this age group, and studies have suggested that it has potential adverse effects on asthmatic airways. More well-designed studies are needed to confirm the initial worrying data, and action must be taken by both medical officers and health authorities to deal with the elephant in the room and curb the vaping pandemic. The aim of this paper is to provide a review of the current knowledge regarding the effect of vaping on adolescents with asthma and to propose actions to restrain this fast-growing trend.
Comparative Study of HVOF Cr3C2–NiCr Coating with Different Bonding Layer on the Interactive Behavior of Fatigue and Corrosion
Authors: He Bing , Zhang Lijie , Yun Xiao , Wang Jing , Zhou Guangzhi , Chen Zhikai , Yuan Xiaoming
Published: February 2022

In order to improve material service life under a fatigue and corrosion coupling environment, a high-velocity oxygen fuel (HVOF) Cr3C2–NiCr coating with a bonding layer was prepared. The objective was to obtain the optimum bonding layer for the HVOF Cr3C2–NiCr coating, which included a laser cladding (LC) Ni625 layer, extreme high-speed laser material deposition (EHLA) Ni625 layer and HVOF NiCr layer. Fatigue properties of the samples with various bonding layers were investigated by means of a four-point bending fatigue test. Electrochemical impedance spectroscopy (EIS) and the salt spray test were executed after the bending fatigue test to simulate the interactive effect of fatigue and corrosion atmosphere. Failure surfaces were characterized by scanning electron microscopy (SEM) and an energy-dispersive spectrometer (EDS) to indicate the details of corrosion products. Corrosive behaviors of samples were adequately demonstrated according to the results, which included the curves of potentiostatic polarization, impedance magnitude and phase degree, and corrosion products. The result showed that the cycles of perforative cracking for the sample with the EHLA Ni625 bonding layer was almost three times than that of the sample with the HVOF NiCr layer. The magnitude of EIS reduced from ~105 to ~103 for the sample after BFT. Eventually, the main improvement mechanism of the HVOF Cr3C2–NiCr coating with the EHLA Ni625 bonding layer was attributed to the grain refinement of the bonding layer and performed a good level of metallurgical bonding with the substrate.
p53 Immunohistochemistry and Mutation Types Mismatching in High-Grade Serous Ovarian Cancer
Authors: Park Eunhyang , Han Hyunho , Choi Sung-Eun , Park Hyunjin , Woo Ha-Young , Jang Mi , Shim Hyo-Sup , Hwang Sohyun , Kang Haeyoun , Cho Nam-Hoon
Published: February 2022

High-grade serous carcinoma (HGSCa) of the ovary is featured by TP53 gene mutation. Missense or nonsense mutation types accompany most cases of HGSCa that correlate well with immunohistochemical (IHC) staining results—an all (missense) or none (nonsense) pattern. However, some IHCs produce subclonal or mosaic patterns from which TP53 mutation types, including the wild type of the gene, cannot be clearly deduced. We analyzed a total of 236 cases of ovarian HGSCa and tumors of other histology by matching the results of p53 IHC staining and targeted next-generation sequencing (TruSight Tumor 170 panel). Ambiguous IHCs that do not belong to the conventional “all or none” groups were reviewed to distinguish the true wild type (WT) from potentially pathogenic subclonal or mosaic patterns. There were about 9% of sequencing-IHC mismatching cases, which were enriched by the p53 c-terminal encoding nuclear localization signal and oligomerization domain, in which the subcellular locations of p53 protein were affected. Indeed, mutations in the oligomerization domain of the p53 protein frequently revealed an unmatched signal or cytosolic staining (L289Ffs*57 (Ins), and R342*). We conclude that both mutation types and IHC patterns of p53 are important sources of information to provide a precise diagnosis of HGSCa.
The Composition and Physical Properties of Clots in COVID-19 Pathology
Authors: Dauwerse Sierk , ten Cate Hugo , Spronk Henri M. H. , Nagy Magdolna
Published: February 2022

Hemostasis is a finely tuned process of which dysregulation can lead either to bleeding or thrombotic complications. The latter is often caused by the hypercoagulable state as it is also seen in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, i.e., in COVID-19 patients. COVID-19 patients requiring hospitalization often suffer from thrombotic events that could not be predicted using routine coagulation assays. Recently, several studies have reported ROtational ThromboElastoMetry (ROTEM) as a promising tool to predict outcomes in COVID-19 patients. In this review we give an overview of ROTEM with a particular focus on the interpretation of the symmetrical clot formation curve in relation to coagulopathy in COVID-19 patients. Furthermore, we have introduced new parameters that might help to better distinguish between COVID-19 patients and outcomes.
Construction of Questionnaire-Scale USOTIC “Social Networks in Primary and Secondary School Students: Use and Digital Coexistence”
Authors: López Berlanga María Carmen , Ortiz Jiménez Luis , Sánchez Romero Cristina
Published: February 2022

In the present work, the construction process, namely the design, elaboration and validation of a questionnaire-scale is described to evaluate the attitudes and interactions in the way of living in the digital world of social networks in primary and secondary students. The current aim was to analyse the means, uses and risks, as well as the sources of training and advice those students have to interact with the diverse range of devices with an internet connection that provide access to social networks. The participating sample for validation was 1073 students from the Primary and Secondary Education stages of centres with organic dependency: Private, concerted and public. The obtained results express the satisfactory metric quality of the questionnaire, thus presenting a theoretically based and operationally defined instrument, considering four relevant dimensions: Special, environmental, perceptual and motivational.
Students’ Depression and School Burnout in the Context of Family Network Acceptance and Deviation from Balanced Time Perspective
Authors: Macałka Emilia , Tomaszek Katarzyna , Kossewska Joanna
Published: February 2022

Nowadays, a growing increase in depression and school burnout is being observed in students. The research aim is to test the mediating effect deviation from balanced time perspective, gender, and subjective sense of family network acceptance on the relationship between depression and school burnout. The sample consisted of 355 students (61.6% girls, 48.4% boys) from the third grade of high school in the south of Poland (mean age 18.5). The student school burnout scale for youth, Time Perspective Inventory, and Depression Scale for youth were used in the survey. Results showed significant relationships between the time perspective indicators, depression, and school burnout as well as a mediation effect of the past time perspective and the fatalistic present time perspective towards depression through school burnout. The findings confirmed that the focus of a person on immediate hedonistic goals becomes significant for the severity of depression only after accounting for the level of school burnout. Conclusions comprised the design of psychological interventions aimed at the group of young people experiencing depression and school burnout considering the time perspective development.
Instructional Perseverance in Early-Childhood Classrooms: Supporting Children’s Development of STEM Reasoning in a Social Justice Context
Authors: Ward Jennifer , DiNapoli Joseph , Monahan Katie
Published: February 2022

In early childhood education (ECE) classrooms, teachers navigate practices about how to allow space for students to make sense of new STEM-based ideas. We posit that such pedagogical moves require ample in-the-moment perseverance by the instructor. In this paper, we seek to explore the nature of such instructional perseverance in ECE classrooms and how it manifests when ECE educators are supporting young children to develop their STEM reasoning, with a primary focus on the mathematics discipline in a social justice context. Working with a dataset consisting of four ECE classroom episodes, we employed an analytical framework that captured evidence of instructional perseverance. We found that the instructional perseverance of the ECE teacher was integral to the development of STEM reasoning of her young students. We present an illustrative case that details the instructional perseverance of the ECE teacher and the related STEM reasoning of her students in the context of exploring income variance by race. We argue that teacher education development must address how ECE teachers can plan for and navigate in-the-moment instructional obstacles in order to support young students’ STEM reasoning development, which positions students for productive STEM-based outcomes.
25,000 fps Computational Ghost Imaging with Ultrafast Structured Illumination
Authors: Huang Hongxu , Li Lijing , Ma Yuxuan , Sun Mingjie
Published: February 2022

Computational ghost imaging, as an alternative photoelectric imaging technology, uses a single-pixel detector with no spatial resolution to capture information and reconstruct the image of a scene. Due to its essentially temporal measurement manner, improving the image frame rate is always a major concern in the research of computational ghost imaging technology. By taking advantage of the fast switching time of LED, an LED array was developed to provide a structured illumination light source in our work, which significantly improves the structured illumination rate in the computational ghost imaging system. The design of the LED array driver circuit presented in this work makes full use of the LED switching time and achieves a pattern displaying rate of 12.5 MHz. Continuous images with 32 × 32 pixel resolution are reconstructed at a frame rate of 25,000 fps, which is approximately 500 times faster than what a universally used digital micromirror device can achieve. The LED array presented in this work can potentially be applied to other techniques requiring high-speed structured illumination, such as fringe 3D profiling and array-based LIFI.
Control Design of Observer-Based Virtual Soft Boundary for a Power-Assist System with Limited Operating Range
Authors: Hsueh Po-Wen , Yao Wu-Sung , Kao Tien-Min
Published: February 2022

Control design of power-assist systems has been widely applied to human-robot interactive systems such as wearable exoskeleton systems, of which the range of motion limitation of human joints in the power-assist systems is essential. This paper presents a virtual soft boundary design for a human-robot cooperation system with a limited operating range. The proposed virtual soft boundary is realized by impedance control and integrated into the power-assist robot arm system; meanwhile, power-assist robot arm systems are typical human-in-the-loop systems, and the control of the power-assist system in performing in accordance with a human’s perception is a significant issue. Therefore, a model-based disturbance observer with a pseudo-derivative feedback feedforward (PDFF) compensator is designed to effectively estimate the human’s torque for an appropriate motor torque command. Experimental results show that the proposed control method can estimate the human torque exerted on the robot arm system to achieve a power-assist system, and the virtual soft boundary can be realized by the impedance control and integrated into the power-assist robot arm system.
A Study on the Relationship between RPE and sEMG in Dynamic Contraction Based on the GPR Method
Authors: Ni Weiguang , Zhang Yuxin , Li Xinyi , Wang Xixi , Wu Yiqi , Liu Guangda
Published: February 2022

The rating of perceived exertion (RPE) and surface electromyography (sEMG) describe exercise intensity subjectively and objectively, while there has been a lack of research on the relationship between them during dynamic contractions to predict exercise intensity, comprehensively. The purpose of this study was to establish a model of the relationship between sEMG and RPE during dynamic exercises. Therefore, 20 healthy male subjects were organized to perform an incremental load test on a cycle ergometer, and the subjects’ RPEs (Borg Scale 6–20) were collected every minute. Additionally, the sEMGs of the subjects’ eight lower limb muscles were collected. The sEMG features based on time domain, frequency domain and time–frequency domain methods were extracted, and the relationship model was established using Gaussian process regression (GPR). The results show that the sEMG and RPE of the selected lower limb muscles are significantly correlated (p < 0.05) but that they have different monotonic correlation degrees. The model that was established with all three domain features displayed optimal performance and when the RPE was 13, the prediction error was the smallest. The study is significant for lower limb muscle training strategy and quantification of training intensity from both subjective and objective aspects, and lays a foundation for sEMG further applications in rehabilitation medicine and sports training.
Emotion Analysis and Dialogue Breakdown Detection in Dialogue of Chat Systems Based on Deep Neural Networks
Authors: Matsumoto Kazuyuki , Sasayama Manabu , Yoshida Minoru , Kita Kenji , Ren Fuji
Published: February 2022

In dialogues between robots or computers and humans, dialogue breakdown analysis is an important tool for achieving better chat dialogues. Conventional dialogue breakdown detection methods focus on semantic variance. Although these methods can detect dialogue breakdowns based on semantic gaps, they cannot always detect emotional breakdowns in dialogues. In chat dialogue systems, emotions are sometimes included in the utterances of the system when responding to the speaker. In this study, we detect emotions from utterances, analyze emotional changes, and use them as the dialogue breakdown feature. The proposed method estimates emotions by utterance unit and generates features by calculating the similarity of the emotions of the utterance and the emotions that have appeared in prior utterances. We employ deep neural networks using sentence distributed representation vectors as the feature. In an evaluation of experimental results, the proposed method achieved a higher dialogue breakdown detection rate when compared to the method using a sentence distributed representation vectors.
A 28 nm Bulk CMOS Fully Digital BPSK Demodulator for US-Powered IMDs Downlink Communications
Authors: Ballo Andrea , Grasso Alfio Dario , Privitera Marco
Published: February 2022

Low-invasive and battery-less implantable medical devices (IMDs) have been increasingly emerging in recent years. The developed solutions in the literature often concentrate on the Bidirectional Data-Link for long-term monitoring devices. Indeed, their ability to collect data and communicate them to the external world, namely Data Up-Link, has revealed a promising solution for bioelectronic medicine. Furthermore, the capacity to control organs such as the brain, nerves, heart-beat and gastrointestinal activities, made up through the manipulation of electrical transducers, could optimise therapeutic protocols and help patients’ pain relief. These kinds of stimulations come from the modulation of a powering signal generated from an externally placed unit coupled to the implanted receivers for power/data exchanging. The established communication is also defined as a Data Down-Link. In this framework, a new solution of the Binary Phase-Shift Keying (BPSK) demodulator is presented in this paper in order to design a robust, low-area, and low-power Down-Link for ultrasound (US)-powered IMDs. The implemented system is fully digital and PLL-free, thus reducing area occupation and making it fully synthesizable. Post-layout simulation results are reported using a 28 nm Bulk CMOS technology provided by TSMC. Using a 2 MHz carrier input signal and an implant depth of 1 cm, the data rate is up to 1.33 Mbit/s with a 50% duty cycle, while the minimum average power consumption is cut-down to 3.3 μW in the typical corner.
Halide Segregation in Mixed Halide Perovskites: Visualization and Mechanisms
Authors: Tian Liuwen , Xue Jingjing , Wang Rui
Published: February 2022

Photoinduced halide segregation in mixed halide perovskites is an intriguing phenomenon and simultaneously a stability issue. In-depth probing this effect and unveiling the underpinning mechanisms are of great interest and significance. This article reviews the progress in visualized investigation of halide segregation, especially light-induced, by means of spatially-resolved imaging techniques. Furthermore, the current understanding of photoinduced phase separation based on several possible mechanisms is summarized and discussed. Finally, the remained open questions and future outlook in this field are outlined.
Grid Synchronization Enhancement of Distributed Generators Using an Adaptive Phase-Locked Loop Tuning System
Authors: Elkholy Ahmed M. , Taha Ibrahim B. M. , Kamel Salah , El-Nemr Mohamed K.
Published: February 2022

This paper proposes an effective algorithm; this algorithm is updating the phase-locked loop (PLL) gains according to the value of the short circuit ratio that will decrease the weak grid effect in the PLL controllers to reduce problems related to renewable energy sources and connect to the point of common coupling (PCC). Based on system parameters estimation, the proposed algorithm enhances the system stability for the distribution networks. The standard IEEE 33-bus test system is used to investigate the proposed algorithm’s effectiveness and superiority. An extensive mathematical effort is exerted to get the system’s small-signal stability (SSS) model. The tuning of the phase-locked loop and the current control loop are choosing to dynamically change according to the system variables to ensure the same system stability margin, whatever the change in the loading conditions. The MATLAB/Simulink model is used to validate the algorithm and simulate the test system.
Impact of Special Drying Schemes on Color Stability of Mangoes with Different Maturity Degrees
Authors: Diop Alioune , Méot Jean-Michel , Léchaudel Mathieu , Chiroleu Frédéric , Ndiaye Nafissatou Diop , Mertz Christian , Cissé Mady , Chillet Marc
Published: February 2022

A previous study demonstrated that the color of 4 mm mango slices is altered very slightly by drying for 5 h at 60 °C, 30% RH and 1 m/s. The objectives of this complementary study were to determine the impact of various drying procedures encountered in the drying units on color alterations of sulfite-free mango slices from heterogeneous raw material due to variable maturity degrees of mangoes. Drying procedures with various temperature/humidity/duration combinations were performed to analyze their effects on the color of natural dried mangoes according to the degree of fruit maturity. They were dried at an air speed of 1.0 m/s for 5 h according to 3 schemes: standard drying (SD) at 60 °C and 30% RH; wet drying (WD) for 1 h at 60 °C and 60% RH, followed by 4 h SD; and finally, hot drying (HD) for 4 h SD, followed by 1 h at 80 °C and 30% RH. The color of the mango slices was analyzed before and after drying. SD preserves the color of fresh mangoes very well, whatever their maturity stage. A relatively slow drying onset corresponding to WD has a highly adverse impact, which becomes greater as the degree of maturity increases. There is already significant browning on mangoes with near-optimum quality (L* = 75; H* = 92). Applying high temperature at the end of the drying procedure (HD) for 20% of the time has a more limited adverse impact with immature mangoes that are the most sensitive. Linear regressions were assessed to represent the relationships of color differences between drying schemes according to mango maturity degrees. These statistical models showed a significant increase in color degradation in the case of WD and a decrease in color differences in the case of HD with the advance in fruit maturity.
Natural Antioxidants and Hydrocolloids as a Mitigation Strategy to Inhibit Advanced Glycation End Products (AGEs) and 5-Hydroxymethylfurfural (HMF) in Butter Cookies
Authors: Hu Huiyu , Wang Yuting , Huang Yousheng , Yu Yanpeng , Shen Mingyue , Li Chang , Nie Shaoping , Xie Mingyong
Published: February 2022

Maillard reaction during food processing contributes to the formation of some unpleasant heat-induced toxicants including advanced glycation end products (AGEs) and 5-hydroxymethylfurfural (HMF). The current study prepared butter cookies fortified with two dietary natural antioxidants (catechins and curcumin) and two dietary hydrocolloids (pectin and chitosan), and investigated their effects on formation of free Nε-(carboxymethyl)lysine (CML)/Nε-(carboxyethyl)lysine (CEL), protein-bound CML/CEL and HMF and on the sensory qualities of butter cookies. Meanwhile, three typical α-dicarbonyl compounds were also determined to identify possible correlations between α-dicarbonyl intermediates and formation of these harmful heat-induced products in butter cookies. Experimental data showed that catechin exhibited the strongest inhibitory effects on formation of AGEs and HMF, but its addition would impair the color and taste of cookies. On the other hand, chitosan was not so effective in inhibiting AGEs and HMF as compared to catechin, but its addition could increase the sensory qualities of butter cookies.
Interaction between Fish Skin Gelatin and Pea Protein at Air-Water Interface after Ultrasound Treatment
Authors: Odelli Davide , Sarigiannidou Krystalia , Soliani Alberto , Marie Rodolphe , Mohammadifar Mohammad Amin , Jessen Flemming , Spigno Giorgia , Vall-llosera Mar , de Carvalho Antonio Fernandes , Verni Michela , Casanova Federico
Published: February 2022

The interaction between fish skin gelatin (FG) and pea protein isolate (PPI) was investigated at the air-water interface (A-W) before and after a high intensity (275 W, 5 min) ultrasound treatment (US). We analyzed the properties of the single protein suspensions as well as an equal ratio of FG:PPI (MIX), in terms of ζ-potential, particle size, molecular weight, bulk viscosity and interfacial tension. The foaming properties were then evaluated by visual analysis and by Turbiscan Tower. Confocal laser scanning microscopy (CLSM) was employed to explore the role of the proteins on the microstructure of foams. The results showed that the ultrasound treatment slightly influenced physicochemical properties of the proteins, while in general, did not significantly affect their behavior both in bulk and at the air-water interface. In particular, PPI aggregate size was reduced (−48 nm) while their negative charges were increased (−1 mV) after the treatment. However, when the proteins were combined, higher molecular weight of aggregates, higher foam stability values (+14%) and lower interfacial tension (IFT) values (47.2 ± 0.2 mN/m) were obtained, leading us to assume that a weak interaction was developed between them.
Assessment of Various Process Parameters for Optimized Sterilization Conditions Using a Multi-Sensing Platform
Authors: Vahidpour Farnoosh , Guthmann Eric , Arreola Julio , Alghazali Yousef , Wagner Torsten , Schöning Michael J.
Published: February 2022

In this study, an online multi-sensing platform was engineered to simultaneously evaluate various process parameters of food package sterilization using gaseous hydrogen peroxide (H2O2). The platform enabled the validation of critical aseptic parameters. In parallel, one series of microbiological count reduction tests was performed using highly resistant spores of B. atrophaeus DSM 675 to act as the reference method for sterility validation. By means of the multi-sensing platform together with microbiological tests, we examined sterilization process parameters to define the most effective conditions with regards to the highest spore kill rate necessary for aseptic packaging. As these parameters are mutually associated, a correlation between different factors was elaborated. The resulting correlation indicated the need for specific conditions regarding the applied H2O2 gas temperature, the gas flow and concentration, the relative humidity and the exposure time. Finally, the novel multi-sensing platform together with the mobile electronic readout setup allowed for the online and on-site monitoring of the sterilization process, selecting the best conditions for sterility and, at the same time, reducing the use of the time-consuming and costly microbiological tests that are currently used in the food package industry.
Facing Food Risk Perception: Influences of Confinement by SARS-CoV-2 Pandemic in Young Population
Authors: Cantalapiedra Fernando , Juan Cristina , Juan-García Ana
Published: February 2022

A new food safety level of trust in food risk perception has been noticed, as a consequence of the SARS-CoV-2 pandemic. The pandemic made-up to review nutritional recommendations for the population, mainly for the young population. Here, the results of a designed survey for the young population, from the University of Valencia, Spain, belonging to grades in the health branch of knowledge, and in charge of carrying out the shopping task for their household, are reported. The study reports three different scenarios and years, as defined by the SARS-CoV-2 pandemic: before the pandemic (period January–December 2019), during the pandemic lockdown (period March 2020–August 2020), and after the pandemic lockdown (September 2020–June 2021). The survey was designed with questions, profiling responses using the best–worst elicitation (BWE) format. Results reported that trust and evaluation of information differed in all three scenarios. In the SARS-CoV-2 pandemic, there was (i) a high increase in trust in the information provided inside (by) the shopping place, while there were no changes for the outside (kept in medium score); (ii) trust in cooperative stakeholders went from a medium-low to medium-high score, while, for individual stakeholders, it was maintained as a medium score, and (iii) trust in information on food products was kept in high score. Regarding the evaluation of the information provided by stakeholders, a tendency in medium score was maintained, while that from the channels of distribution went from medium-low to medium-high for buying on-site. A uniform tendency was observed for online/other distribution channels for all three years and descriptors studied: “Internet”, “Farmer on-demand”, and “Cooperative consumers” (<50%). This research provides findings of implications that contribute to changing the perception of food risk, due to the COVID-19 pandemic, i.e., the adaptation of the young population, trust in safety and quality, and importance of coordination from all communication points to avoid negative or strongest consequences, in case of future lockdowns or health crisis.
Assessment of “Sugranineteen” Table Grape Maturation Using Destructive and Auto-Fluorescence Methods
Authors: Hamie Najwane , Tarricone Luigi , Verrastro Vincenzo , Natrella Giuseppe , Faccia Michele , Gambacorta Giuseppe
Published: February 2022

The optimal harvesting of table grapes is commonly determined based on technological and phenolic indices analyzed over the course of its maturity. The classical techniques used for these analyses are destructive, time-consuming, and work for a limited number of samples that may not represent the heterogeneity of the vineyard. This study aimed to follow the ripening season of table grapes using non-destructive tools as a rapid and accurate alternative for destructive techniques. Grape samples were collected from a Sugranineteen vineyard during the ripening season to measure the basic maturity indices via wet chemistry, and total polyphenols, anthocyanins, and flavonoids were evaluated by spectrophotometry. Fluorescent readings were collected from intact clusters with a portable optical sensor (Multiplex® 3, Force-A, France) that generates indices correlated to different maturity parameters. Results revealed strong relationships between the Multiplex® indices ANTH_RG and FERARI and the skin anthocyanin content, with R2 values equal to 0.9613 and 0.8713, respectively. The NBI_R index was also related to total anthocyanins (R2 = 0.8032), while the SFR_R index was linked to the titratable acidity (R2 = 0.6186), the sugar content (R2 = 0.7954), and to the color index of red grapes (CIRG) (R2 = 0.7835). Results demonstrated that Multiplex® 3 can be applied on intact clusters as an effective non-destructive tool for a rapid estimation of table grapes’ anthocyanin content.
Effect of Protein Thermal Denaturation on the Texture Profile Evolution of Beijing Roast Duck
Authors: Liu Yanxia , Wang Zhenyu , Zhang Dequan , Pan Teng , Liu Huan , Shen Qingwu , Hui Teng
Published: February 2022

To investigate the mechanism of the texture formed by protein thermal denaturation, the profile and formation of texture and thermal denaturation of protein were evaluated using texture profile analysis (TPA) and transmission electron microscopy (TEM) combined with differential scanning calorimeter (DSC). Results indicated that the surface temperature of Beijing roast duck increased from 23.9 to 174.4 °C, while the center temperature rose from 20.6 to 99.3 °C during roasting. Shear force decreased significantly during the first 20 min, and the texture profile largely changed at 20 and 40 min. Firstly, Band I was broken and twisted, Band A was overstruck, and Z-line was diffused and finally disappeared, resulting in a blurred myofibril structure. The sarcomere considerably contracted within 30 min. Secondly, the main myofibrillar proteins were denatured at 20 and 40 min, respectively. The formation of hydrophobic interactions and the reduction of ionic bonds were observed. Thirdly, roasting induced protein thermal denaturation, which was correlated with interprotein forces, texture profile, and the shear force. Muscle fibers were damaged and shrunken, accompanied by the formation of hydrophobic interactions and the reduction of ionic bonds. The turning points were at 20 and 40 min, and the main proteins were denatured, leading to the formation of tenderness of Beijing roast duck.
Comprehensive Studies of Different Cancer Diseases among Less-Developed Countries
Authors: Rahman Mohammed M. , Opo Firoz A. D. M. , Asiri Abdullah M.
Published: February 2022

Recently, the rate of cancer deaths in less-developed countries such as Bangladesh has significantly increased day by day, making it a major health issue. The most predominant types of cancers among the populations of less-developed countries (especially Bangladesh) are lung, throat, colon, gastric, ovarian, breast, and skin cancers. The mortality rate is increasing for both males and females. The main common factors are smoking, use of tobacco leaves, bacterial or viral infection, hereditary disorders, food adulterations, and environmental factors, which are highly responsible for the development of carcinoma in the young to adult population in this region. Raising consciousness among people regarding early diagnosis, decreasing the use of chemicals such as formalin for food preservation, and reducing environmental pollution such as arsenic as well as air pollution might help to reduce the number of deaths. Education and public campaigns can also reduce the intensity of cancer occurrence. Breast, esophagus, and cervical cancer are common diseases in less-developed countries such as Bangladesh.
Pomological Traits and Genome Size of Prunus armeniaca L. Considering to Geographical Origin
Authors: Rampáčková Eliška , Mrázová Martina , Čížková Jana , Nečas Tomáš
Published: February 2022

Apricot (Prunus armeniaca L.) is an important fruit crop and member of the Prunus genus of the Rosaceae family that is planted in many temperate regions worldwide. The quality of fruit is assessed by many pomological parameters which can serve as a decisive factor in apricot breeding, because the introduction of new cultivars is required. These parameters can differ with climate conditions, geographical location or geographic ecological origin. Similarly, another biological characteristic can be measured depending on these terms. The present study was conducted with the aim of estimating pomological traits together with the nuclear DNA content of 35 apricot cultivars with different geographical origins. Only CV values lower than 5% were considered in flow cytometry analysis. All analyzed cultivars were diploid and the genome size value ranged from 0.587 to 0.644 pg/2C, where Turkish apricots reached the highest value (on average 0.628 pg/2C) followed by the European group (on average 0.625 pg/2C). A Spearman-rank correlation was used and the different correlation was found for specific geographical groups of apricot cultivars. The genome size values of apricots and related botanical species P. mume, P. sibirica and P. ansu showed to be very similar values.
Patient with Phenylketonuria and Intellectual Disability—Problem Not Always Caused Exclusively by Insufficient Metabolic Control (Coexistence of PKU and Alazami Syndrome)
Authors: Patalan Michał , Leśniak Alicja , Bernatowicz Krzysztof , Romanowska Hanna , Krzywińska-Zdeb Elżbieta , Walczak Mieczysław , Giżewska Maria
Published: February 2022

The authors present a case report of a boy with a classical form of phenylketonuria and Alazami syndrome. The inborn error of phenylalanine metabolism was diagnosed in the neonatal period based on population new-born screening. Despite early implementation of a low-phenylalanine diet and good biochemical control, the patient developed behavioural disorders and intellectual disability. He also presented with dysmorphic features. After long and extensive attempts to establish the genetic cause of this unusual phenotype, finally, at the age of 16 the boy was diagnosed with Alazami syndrome based on whole exome sequencing. The authors discussed the problem of neuropsychological disorders in patients with phenylketonuria and described typical clinical symptoms of Alazami syndrome. It was emphasized that the presence of one monogenic disease does not exclude the coexistence of another one.
Distinguishing Different Types of Mobile Phone Addiction: Development and Validation of the Mobile Phone Addiction Type Scale (MPATS) in Adolescents and Young Adults
Authors: Liu Qing-Qi , Xu Xiao-Pan , Yang Xiu-Juan , Xiong Jie , Hu Yu-Ting
Published: February 2022

Researchers have developed various versions of scales to measure mobile phone addiction. Existing scales, however, focus primarily on the overall level of mobile phone addiction but do not distinguish the potential differences between different types of mobile phone addiction. There is a lack of established scales that can measure different types of mobile phone addiction. The present study aimed to uncover the specific types of mobile phone addiction and develop a Mobile Phone Addiction Type Scale (MPATS) for adolescents and young adults. Adolescents and young adults from two high schools and two universities in Central and South China participated in our study. A total of 108 mobile phone addicts (Mage = 17.60 years, SD = 3.568 years; 60.185% males) were interviewed to uncover the specific types of mobile phone addiction. Data from 876 adolescents and young adults (Mage = 16.750 years, SD = 3.159 years; 49.087% males) were tested for item discrimination and exploratory factor analysis. Data from 854 adolescents and young adults (Mage = 16.750 years, SD = 3.098 years; 50.820% males) were analyzed for construct validity, convergent validity, criterion-related validity, and internal consistency reliability. The 26-item Mobile Phone Addiction Type Scale (MPATS) was developed with four factors named mobile social networking addiction, mobile game addiction, mobile information acquisition addiction, and mobile short-form video addiction. The four-factor, 26-item MPATS revealed good construct validity, convergent validity, criterion-related validity, and internal consistency reliability. The new scale is suitable for measuring different types of mobile phone addiction in adolescents and young adults. Limitations and implications are discussed.
The Role of Medical Mistrust in Concerns about Tumor Genomic Profiling among Black and African American Cancer Patients
Authors: Hoadley Ariel , Bass Sarah Bauerle , Chertock Yana , Brajuha Jesse , D’Avanzo Paul , Kelly Patrick J. , Hall Michael J.
Published: February 2022

Tumor genomic profiling (TGP) is used in oncology practice to optimize cancer treatment and improve survival rates. However, TGP is underutilized among Black and African American (AA) patients, creating potential disparities in cancer treatment outcomes. Cost, accuracy, and privacy are barriers to genetic testing, but medical mistrust (MM) may also influence how Black and AA cancer patients perceive TGP. From December 2019 to February 2020, 112 Black and AA adults from two outpatient oncology sites in Philadelphia, PA without a known history of having TGP testing conducted completed a cross-sectional survey. Items queried included sociodemographic characteristics, clinical factors, patient–oncologist relationship quality, medical mistrust, and concerns about TGP. A k-means cluster analysis revealed two distinct psychographic clusters: high (MM-H) versus low (MM-L) medical mistrust. Clusters were not associated with any sociodemographic or clinical factors, except for age (MM-H patients older than MM-L patients, p = 0.006). Eleven TGP concerns were assessed; MM-H patients expressed greater concerns than MM-L patients, including distrust of the government, insurance carriers, and pharmaceutical companies. TGP concerns varied significantly based on level of medical mistrust, irrespective of sociodemographic characteristics. Targeted communications addressing TGP concerns may mitigate disparities in TGP uptake among those with medical mistrust.
Telework and Mental Health during COVID-19
Authors: Mendonça Inês , Coelho Franz , Ferrajão Paulo , Abreu Ana Maria
Published: February 2022

COVID-19 has come to change societal organization. Due to lockdowns, work typologies have been rethought and telework has gained strength. However, the impact of the constant use of information and communication technologies on the mental health of workers needs to be considered. We aimed to investigate the impact of different work conditions on mental health, to which end we disseminated an online questionnaire during lockdowns to assess imagined surveillance, mobile maintenance expectation, communication overload, feelings of entrapment, depression, anxiety, stress, and flourishing in four groups (employed in telework, employed on-site, employed in layoff, and unemployed). We computed mean comparisons and serial mediations. We show that depression and anxiety were more prevalent in women; parents flourished more than people without children; and people with a higher level of education feel more entrapment. Crucially, we show that telework was associated with imagined surveillance and communication overload, which mediated the association with mobile maintenance expectations and entrapment (which was exacerbated by parenthood), impacting mental health and the quality of life. However, this was also partially observed in the remaining work conditions. Finally, flourishing worked as a protector against mental health issues in all work conditions. We discuss this given the massification of digital migration.
Co-Creation Hub Is the First Step for the Successful Creation of a Unified Urban Ecosystem-Kaunas City Example
Authors: Feiferytė-Skirienė Akvilė , Draudvilienė Lina , Stasiškienė Žaneta , Sosunkevič Sergej , Pamakštys Kastytis , Daniusevičiūtė-Brazaitė Laura , Gurauskienė Inga
Published: February 2022

The identification of the main steps for the creation of a unified ecosystem from the institutional point of view and the framework for ecosystem design is presented and discussed. Based on the expertise and the knowledge gained during the time when the ELISE project had been implemented, a unified Kaunas city ecosystem is being designed using the Ecosystem Map method. As the review of the ELISE project reports helped to identify the main steps of each project partner in building ecosystems’ networks, Kaunas city chose to create a co-Creation Hub (c-CH), which is the first step in developing an ecosystem management model. The main tasks of such a hub are listed, and should involve the preparation of a long-term action plan involving not only the coordination of the stakeholder meetings, organisation of seminars, the preparation of new materials, and methodology but also the development of a clear strategy for each stakeholder based on national economy and government and municipality policies. The role of the c-CH is to ensure the ease of cooperation and knowledge distribution among stakeholders within the city, public authorities, and the national government. This approach could become a fundamental background tool for the regional and/or city municipal and stakeholder-based creation and development of unified ecosystem development.