bluuebunny/crossref_metadata_2025 huggingface dataset:
Different Inhibitory Effects of Erythromycin and Chlortetracycline on Early Growth of Brassica campestris Seedlings
Authors: Cheong Mi Sun , Choe Hyeonji , Jeong Myeong Seon , Yoon Young-Eun , Jung Hyun Suk , Lee Yong Bok
Published: October 2021

Veterinary antibiotics, including erythromycin (Ery) and chlortetracycline (CTC), are often detected in agricultural land. Although these contaminants affect plant growth and development, their effects on crops remain elusive. In this study, the effects of Ery and CTC on plant growth were investigated and compared by analyzing transcript abundance in Brassica campestris seedlings. Treatment with Ery and/or CTC reduced chlorophyll content in leaves and photosynthetic efficiency. Examination of the chloroplast ultrastructure revealed the presence of abnormally shaped plastids in response to Ery and CTC treatments. The antibiotics produced similar phenotypes of lower accumulation of photosynthetic genes, including RBCL and LHCB1.1. Analysis of the transcript levels revealed that Ery and CTC differentially down-regulated genes involved in the tetrapyrrole biosynthetic pathway and primary root growth. In the presence of Ery and CTC, chloroplasts were undeveloped and photosynthesis efficiency was reduced. These results suggest that both Ery and CTC individually affect gene expression and influence plant physiological activity, independently of one another.
CRIF1 Deficiency Increased Homocysteine Production by Disrupting Dihydrofolate Reductase Expression in Vascular Endothelial Cells
Authors: Lee Ikjun , Piao Shuyu , Kim Seonhee , Nagar Harsha , Choi Su-Jeong , Jeon Byeong Hwa , Oh Sang-Ha , Irani Kaikobad , Kim Cuk-Seong
Published: October 2021

Elevated plasma homocysteine levels can induce vascular endothelial dysfunction; however, the mechanisms regulating homocysteine metabolism in impaired endothelial cells are currently unclear. In this study, we deleted the essential mitoribosomal gene CR6 interacting factor 1 (CRIF1) in human umbilical vein endothelial cells (HUVECs) and mice to induce endothelial cell dysfunction; then, we monitored homocysteine accumulation. We found that CRIF1 downregulation caused significant increases in intracellular and plasma concentrations of homocysteine, which were associated with decreased levels of folate cycle intermediates such as 5-methyltetrahydrofolate (MTHF) and tetrahydrofolate (THF). Moreover, dihydrofolate reductase (DHFR), a key enzyme in folate-mediated metabolism, exhibited impaired activity and decreased protein expression in CRIF1 knockdown endothelial cells. Supplementation with folic acid did not restore DHFR expression levels or MTHF and homocysteine concentrations in endothelial cells with a CRIF1 deletion or DHFR knockdown. However, the overexpression of DHFR in CRIF1 knockdown endothelial cells resulted in decreased accumulation of homocysteine. Taken together, our findings suggest that CRIF1-deleted endothelial cells accumulated more homocysteine, compared with control cells; this was primarily mediated by the disruption of DHFR expression.
Design of Supply Chain System Based on Blockchain Technology
Authors: Li Jing , Song Yafei
Published: October 2021

As the interaction between companies becomes more and more complex, the problems of asymmetric information, weak traceability, and low collaboration efficiency in the traditional centralized supply chain are becoming increasingly prominent. To solve these problems, this paper designs a supply chain system based on blockchain. With the help of trade chain and information chain platforms, an overall framework of the supply chain system is constructed. By formulating platform interaction rules, the system information exchange format is standardized to ensure the stability and efficiency of system interaction. Smart contracts are used to manage supply chain system transactions and information interactions to achieve efficient and convenient information sharing, ensuring the security and reliability of supply chain information. The comprehensive performance of the system is evaluated through experiments. Experimental results indicate that while the system realizes the basic functions of the supply chain, it can promote the sharing of information between participants and improve its efficiency.
Modelling Strategies for the Numerical Simulation of the Behaviour of Corroded RC Columns under Cyclic Loads
Authors: Molaioni Filippo , Di Carlo Fabio , Rinaldi Zila
Published: October 2021

Rebars corrosion phenomena can modify the structural behaviour of reinforced concrete (RC) members and consequently the seismic performance of RC structures. Since many existing RC structures are affected by this phenomenon, the influence of the reinforcement corrosion on the seismic performance is still under examination, especially when the corrosive attack is localized in the dissipative areas of the plastic hinges. In this work, the effect of localized corrosion is numerically investigated, through the adoption of a suitable finite element model, object of validation with the outcomes of an experimental campaign carried out in the Laboratory of the University of Rome “Tor Vergata”, on un-corroded and corroded RC columns subjected to axial load and cyclic horizontal actions. Particular attention has been paid to the definition of the three-dimensional model and to the modelling of the corroded rebars and their corrosion morphology. Indeed, different modelling strategies are proposed with the aim to properly simulate the cyclic behaviour of the corroded columns. The main results show how more refined strategies taking into account the morphological aspects of the corrosion phenomenon produce a better fit with the experimental results for both Damage Control and Life Safety limit states performance.
Feasibility of Using Nanosilanes in a New Hybrid Stabilised Soil Solution in Rural and Low-Volume Roads
Authors: Díaz-López José Luis , Cabrera Manuel , Marcobal José Ramón , Agrela Francisco , Rosales Julia
Published: October 2021

The application of new materials for soil stabilisation is a growing field of study in recent years. In this work, the effect of two types of silica-based nanomaterials combined with binders (quicklime and cement) are studied to stabilise soils and form structural layers for rural and low volume roads. The physical and chemical properties of the materials have been determined, as well as the mechanical behaviour of the stabilised soil. Three hybrid stabilised soil sections have been designed using a multilayer elastic model, executed at full scale and measuring the evolution of their properties in the medium to short term. The results show that the application of silica-based nanomaterials and two types of binders on the tread layers provide high structural stability and good behaviour of the sections.
Security Challenges in Industry 4.0 PLC Systems
Authors: Hajda Janusz , Jakuszewski Ryszard , Ogonowski Szymon
Published: October 2021

The concept of the fourth industrial revolution assumes the integration of people and digitally controlled machines with the Internet and information technologies. At the end of 2015, more than 20 billion machines and devices were connected to the Internet, with an expected growth to half a trillion by 2030. The most important raw material for this digital revolution is data, which when properly stored, analyzed and secured, constitute the basis for the development of any business. In times of rapid industrial development, automation of production processes and systems integration via networks, the effective protection of the cyber-physical systems of a plant is particularly important. To minimize the risks associated with Internet access, one must define all the possible threats and determine their sources in the plant and block or minimize the possibility of sabotage or data loss. This article analyzes the security measures used in industrial systems. In particular, risk management and the study of the risk sources in terms of human, hardware and software aspects in networked PLC and SCADA systems are discussed. Methods of improving the architecture of industrial networks and their management are proposed in order to increase the level of security. Additionally, the safety of the communication protocols with PLCs in industrial control systems is discussed.
State-Burst Feedback Control for Fault Recovery of Input/State Asynchronous Sequential Machines
Authors: Yang Jung-Min , Park Seong-Jin , Kwak Seong Woo
Published: October 2021

Static corrective controllers are more efficient than dynamic ones since they consist of only logic elements, whereas their existence conditions are more restrictive. In this paper, we present a static corrective control scheme for fault diagnosis and fault tolerant control of input/state asynchronous sequential machines (ASMs) vulnerable to transient faults. The design flexibility of static controllers is enlarged by virtue of using a diagnoser and state bursts. Necessary and sufficient conditions for the existence of a diagnoser and static fault tolerant controller are presented, and the process of controller synthesis is addressed based on the derived condition. Illustrative examples on practical ASMs are provided to show the applicability of the proposed scheme.
Privacy-Preserving Solutions in Blockchain-Enabled Internet of Vehicles
Authors: Kaltakis Konstantinos , Polyzi Panagiota , Drosatos George , Rantos Konstantinos
Published: October 2021

Blockchain, a promising technology that has matured and nowadays is widely used in many fields, such as supply chain management, smart grids, agriculture and logistics, has also been proposed for the Internet of Vehicles (IoV) ecosystem to enhance the protection of the data that roadside units and vehicles exchange. Blockchain technology can inherently guarantee the availability, integrity and immutability of data stored in IoV, yet it cannot protect privacy and data confidentiality on its own. As such, solutions that utilise this technology have to consider the adoption of privacy-preserving schemes to address users’ privacy concerns. This paper provides a literature review of proposed solutions that provide different vehicular services using blockchain technology while preserving privacy. In this context, it analyses existing solutions’ main characteristics and properties to provide a comprehensive and critical overview and identifies their contribution in the field. Moreover, it provides suggestions to researchers for future work in the field of privacy-preserving blockchain-enabled solutions for vehicular networks.
Household Air Pollution and Respiratory Health in Rural Crete, Greece: A Cross-Sectional FRESH AIR Study
Authors: Anastasaki Marilena , Tsiligianni Ioanna , Sifaki-Pistolla Dimitra , Chatzea Vasiliki Eirini , Karelis Andreas , Bertsias Antonios , Chavannes Niels H. , van Gemert Frederik , Lionis Christos ,   the FRESH AIR Collaborators
Published: October 2021

Breathing polluted air is a risk to respiratory conditions. During the Greek financial crisis, the use of household fireplaces/wood stoves shifted from mostly decorative to actual domestic heating, resulting in increased indoor smoke production. We aimed to evaluate household air pollution (HAP), fuel use and respiratory symptoms in rural Crete, Greece. PM2.5 and CO were measured in 32 purposively selected rural households (cross-sectional study) at periods reflecting lesser (baseline) versus extensive (follow-up) heating. Clinical outcomes were assessed using questionnaires. Mean PM2.5 were not significantly different between measurements (36.34 µg/m3 vs. 54.38 µg/m3, p = 0.60) but exceeded the WHO air quality guidelines. Mean and maximal CO levels were below the WHO cut-offs (0.56 ppm vs. 0.34 ppm, p = 0.414 and 26.1 ppm vs. 9.72 ppm, p = 0.007, respectively). In total, 90.6% of households were using wood stoves or fireplaces for heating, but half also owned clean fuel devices. The differences between devices that were owned versus those that were used were attributed to financial reasons. In both cases, the most frequent respiratory symptoms were phlegm (27.3% vs. 15.2%; p = 0.34) and cough (24.2% vs. 12.1%; p = 0.22). Our findings demonstrate the magnitude of HAP and confirm the return to harmful practices during Greece’s austerity. Upon validation, these results can support strategies for fighting fuel poverty, empowering communities and strengthening local health systems.
Finite Arithmetic Axiomatization for the Basis of Hyperrational Non-Standard Analysis
Authors: Lovyagin Yuri N. , Lovyagin Nikita Yu.
Published: October 2021

The standard elementary number theory is not a finite axiomatic system due to the presence of the induction axiom scheme. Absence of a finite axiomatic system is not an obstacle for most tasks, but may be considered as imperfect since the induction is strongly associated with the presence of set theory external to the axiomatic system. Also in the case of logic approach to the artificial intelligence problems presence of a finite number of basic axioms and states is important. Axiomatic hyperrational analysis is the axiomatic system of hyperrational number field. The properties of hyperrational numbers and functions allow them to be used to model real numbers and functions of classical elementary mathematical analysis. However hyperrational analysis is based on well-known non-finite hyperarithmetic axiomatics. In the article we present a new finite first-order arithmetic theory designed to be the basis of the axiomatic hyperrational analysis and, as a consequence, mathematical analysis in general as a basis for all mathematical application including AI problems. It is shown that this axiomatics meet the requirements, i.e., it could be used as the basis of an axiomatic hyperrational analysis. The article in effect completes the foundation of axiomatic hyperrational analysis without calling in an arithmetic extension, since in the framework of the presented theory infinite numbers arise without invoking any new constants. The proposed system describes a class of numbers in which infinite numbers exist as natural objects of the theory itself. We also do not appeal to any “enveloping” set theory.
On Periodic Fractional (p, q)-Integral Boundary Value Problems for Sequential Fractional (p, q)-Integrodifference Equations
Authors: Soontharanon Jarunee , Sitthiwirattham Thanin
Published: October 2021

We study the existence results of a fractional (p, q)-integrodifference equation with periodic fractional (p, q)-integral boundary condition by using Banach and Schauder’s fixed point theorems. Some properties of (p, q)-integral are also presented in this paper as a tool for our calculations.
Sex Associated Effects of Noise Pollution in Stone Sculpin (Paracottus knerii) as a Model Object in the Context of Human-Induced Rapid Environmental Change
Authors: Sapozhnikova Yulia P. , Koroleva Anastasia G. , Yakhnenko Vera M. , Khanaev Igor V. , Glyzina Olga Yu. , Avezova Tatyana N. , Volkova Aleksandra A. , Mushinskaya Angela V. , Tyagun Marina L. , Shagun Artem N. , Makarov Mikhail M. , Kirilchik Sergey V. , Sudakov Nikolay P. , Klimenkov Igor V. , Sukhanova Lyubov V.
Published: October 2021

This work simulates the consequences of HIREC using stone sculpins as model organisms. Sex-dependent effects of long-term noise exposure at mean sound pressure levels of 160–179 dB re 1 μPa (SPLpk–pk) were measured. We applied a multilevel approach to testing the stress response: a comparative analysis of the macula sacculi and an assessment of hematological and molecular stress responses. Noise exposure resulted in hair cell loss, changes in some cytometric parameters in blood, and an increase in the number of functionally active mitochondria in the red blood cells of males and its decrease in females, demonstrating a mitochondrial allostatic load and depletion of functional reserve. Finally, a statistically significant decrease in the telomerase activity of the auditory epithelium and a shortening of telomere length in the brain as molecular markers of stress were observed after noise exposure only in females. No significant decrease in telomerase activity and shortening of telomere length in nerve target tissues were observed in stressed males. However, we recorded an increase in the telomerase activity in male gonads. This sex-dependent difference in load may be associated with accelerated cellular aging in females and lower stress-related long-term risk in males. In this article, we discuss possible reasons for these noise-induced stress effects.
Human Drug Pollution in the Aquatic System: The Biochemical Responses of Danio rerio Adults
Authors: Mauro Manuela , Lazzara Valentina , Arizza Vincenzo , Luparello Claudio , Ferrantelli Vincenzo , Cammilleri Gaetano , Inguglia Luigi , Vazzana Mirella
Published: October 2021

To date, drug pollution in aquatic systems is an urgent issue, and Danio rerio is a model organism to study the toxicological effects of environmental pollutants. The scientific literature has analyzed the effect of human drug pollution on the biochemical responses in the tissues of D. rerio adults. However, the information is still scarce and conflicting, making it difficult to understand its real impact. The scientific studies are not consistent with each other and, until now, no one has grouped their results to create a baseline of knowledge of the possible impacts. In this review, the analysis of literature data highlights that the effects of drugs on adult zebrafishes depend on various factors, such as the tissue analyzed, the drug concentration and the sex of the individuals. Furthermore, the most influenced biochemical responses concern enzymes (e.g., antioxidants and hydrolase enzymes) and total protein and hormonal levels. Pinpointing the situation to date would improve the understanding of the chronic effects of human drug pollution, helping both to reduce it in the aquatic systems and then to draw up regulations to control this type of pollution.
Quantifying Leaf Trait Covariations and Their Relationships with Plant Adaptation Strategies along an Aridity Gradient
Authors: Yang Yanzheng , Kang Le , Zhao Jun , Qi Ning , Li Ruonan , Wen Zhongming , Kassout Jalal , Peng Changhui , Lin Guanghui , Zheng Hua
Published: October 2021

A trait-based approach is an effective way to quantify plant adaptation strategies in response to changing environments. Single trait variations have been well depicted before; however, multi-trait covariations and their roles in shaping plant adaptation strategies along aridity gradients remain unclear. The purpose of this study was to reveal multi-trait covariation characteristics, their controls and their relevance to plant adaptation strategies. Using eight relevant plant functional traits and multivariate statistical approaches, we found the following: (1) the eight studied traits show evident covariation characteristics and could be grouped into four functional dimensions linked to plant strategies, namely energy balance, resource acquisition, resource investment and water use efficiency; (2) leaf area (LA) together with traits related to the leaf economic spectrum, including leaf nitrogen content per area (Narea), leaf nitrogen per mass (Nmass) and leaf dry mass per area (LMA), covaried along the aridity gradient (represented by the moisture index, MI) and dominated the trait–environmental change axis; (3) together, climate, soil and family can explain 50.4% of trait covariations; thus, vegetation succession along the aridity gradient cannot be neglected in trait covariations. Our findings provide novel perspectives toward a better understanding of plant adaptations to arid conditions and serve as a reference for vegetation restoration and management programs in arid regions.
Haematological, Biochemical and Hormonal Biomarkers of Heat Intolerance in Military Personnel
Authors: Alele Faith O. , Malau-Aduli Bunmi S. , Malau-Aduli Aduli E. O. , Crowe Melissa J.
Published: October 2021

Heat intolerance is the inability to withstand heat stress and this may occur due to exertional heat stroke (EHS). However, it is unknown if heat intolerance is associated with immune and hormonal disturbances. This study investigates haematological, biochemical and hormonal biomarkers related to heat intolerance and EHS in military and civilian volunteers. A quasi-experimental pre-and post-test design was used, with participants drawn from the Australian Defence Force (ADF) and the general populace. Blood samples were collected and analysed for biomarkers. Inferential statistics compared the biomarkers between the groups. Changes in alanine amino transaminase (p = 0.034), creatine kinase (0.044), cortisol (p = 0.041) and creatinine (p < 0.001) differed between the heat-intolerant and heat-tolerant groups. Participants with a history of EHS showed significant changes in creatinine (p = 0.022) and urea (p = 0.0031) compared to those without EHS history. Predictors of heat intolerance were increasing post-HTT creatinine and cortisol (OR = 1.177, p = 0.011 and OR = 1.015, p = 0.003 respectively). Conclusively, EHS history is associated with changes in creatinine and urea concentrations, while the predictors of heat intolerance are creatinine and cortisol. However, further exploration of other biomarkers, such as genetic polymorphism, is needed.
Efficacy and Safety of Filgrastim and Its Biosimilars to Prevent Febrile Neutropenia in Cancer Patients: A Prospective Study and Meta-Analysis
Authors: Rastogi Shruti , Kalaiselvan Vivekananda , Ali Sher , Ahmad Ajaz , Guru Sameer Ahmad , Sarwat Maryam
Published: October 2021

Background: The aim of this review and meta-analysis was to identify, assess, meta-analyze and summarize the comparative effectiveness and safety of filgrastim in head-to-head trials with placebo/no treatment, pegfilgrastim (and biosimilar filgrastim to update advances in the field. Methods: The preferred reporting items for systematic reviews and meta-analyses PRISMA statement were applied, and a random-effect model was used. Primary endpoints were the rate and duration of grade 3 or 4 neutropenia, and an incidence rate of febrile neutropenia. Secondary endpoints were time to absolute neutrophil count ANC recovery, depth of ANC nadir (lowest ANC), neutropenia-related hospitalization and other neutropenia-related complications. For filgrastim versus biosimilar filgrastim comparison, the primary efficacy endpoint was the mean difference in duration of severe neutropenia DSN. Results: A total of 56 studies were considered that included data from 13,058 cancer patients. The risk of febrile neutropenia in filgrastim versus placebo/no treatment was not statistically different. The risk ratio for febrile neutropenia was 0.58, a 42% reduction in favor of filgrastim. The most reported adverse event with FIL was bone pain. For pegfilgrastim versus filgrastim, no statistically significant difference was noted. The risk ratio was 0.90 (95% CI 0.67 to 1.12). The overall difference in duration of severe neutropenia between filgrastim and biosimilar filgrastim was not statistically significant. The risk ratio was 1.03 (95% CI 0.93 to 1.13). Conclusions: Filgrastim was effective and safe in reducing febrile neutropenia and related complications, compared to placebo/no treatment. No notable differences were found between pegfilgrastim and filgrastim in terms of efficacy and safety. However, a similar efficacy profile was observed with FIL and its biosimilars.
Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury
Authors: Rosero Salazar Doris H. , van Rheden René E. M. , van Hulzen Manon , Carvajal Monroy Paola L. , Wagener Frank A. D. T. G. , Von den Hoff Johannes W.
Published: October 2021

This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 ± 7.73% fibrin only and 21.11 ± 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 ± 8.89%, p < 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group (p ˂ 0.05). The areas of myofiber formation in the fibrin only group (31.77 ± 10.81%) and fibrin with laminin-nidogen group (43.13 ± 10.39%) were larger than in the empty wounds (28.10 ± 11.68%, p ˂ 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.
Impact of Protein Corona on the Biological Identity of Nanomedicine: Understanding the Fate of Nanomaterials in the Biological Milieu
Authors: Akhter Md Habban , Khalilullah Habibullah , Gupta Manish , Alfaleh Mohamed A. , Alhakamy Nabil A. , Riadi Yassine , Md Shadab
Published: October 2021

Nanoparticles (NPs) in contact with a biological medium are rapidly comprehended by a number of protein molecules resulting in the formation of an NP–protein complex called protein corona (PC). The cell sees the protein-coated NPs as the synthetic identity is masked by protein surfacing. The PC formation ultimately has a substantial impact on various biological processes including drug release, drug targeting, cell recognition, biodistribution, cellular uptake, and therapeutic efficacy. Further, the composition of PC is largely influenced by the physico-chemical properties of NPs viz. the size, shape, surface charge, and surface chemistry in the biological milieu. However, the change in the biological responses of the new substrate depends on the quantity of protein access by the NPs. The PC-layered NPs act as new biological entities and are recognized as different targeting agents for the receptor-mediated ingress of therapeutics in the biological cells. The corona-enveloped NPs have both pros and cons in the biological system. The review provides a brief insight into the impact of biomolecules on nanomaterials carrying cargos and their ultimate fate in the biological milieu.
The Diagnostic, Prognostic and Therapeutic Role of miRNAs in Adrenocortical Carcinoma: A Systematic Review
Authors: Mytareli Chrysoula , Delivanis Danae A. , Athanassouli Fani , Kalotychou Vassiliki , Mantzourani Marina , Kassi Eva , Angelousi Anna
Published: October 2021

Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with a dismal prognosis and a high rate of recurrence and mortality. Therapeutic options are limited. In some cases, the distinction of ACCs from benign adrenal neoplasms with the existing widely available pathological and histopathological tools is difficult. Thus, new biomarkers have been tested. We conducted a review of the recent literature on the advances of the diagnostic, prognostic and therapeutic role of miRNAs on ACC patients. More than 10 miRNAs validated by multiple studies were found to present a diagnostic and prognostic role for ACC patients, from which miR-483-5p and miR-195 were the most frequently met biomarkers. In particular, upregulation of miR-483-5p and downregulation of miR-195 were the most commonly validated molecular alterations. Unfortunately, data on the therapeutic role of miRNA are still scarce and limited mainly at the experimental level. Thus, the role of miRNA regulation in ACC remains an area of active research.
Simultaneous Measurement of Changes in Neutrophil Granulocyte Membrane Potential, Intracellular pH, and Cell Size by Multiparametric Flow Cytometry
Authors: Stratmann Alexander Elias Paul , Wohlgemuth Lisa , Erber Maike Elisabeth , Bernhard Stefan , Hug Stefan , Fauler Michael , Vidoni Laura , Mohamed Adam Omar Khalaf , Thomaß Bertram Dietrich , Münnich Frederik , Stukan Laura , Föhr Karl Josef , Mannes Marco , Huber-Lang Markus Stefan , Messerer David Alexander Christian
Published: October 2021

Neutrophils provide rapid and efficient defense mechanisms against invading pathogens. Upon stimulation with proinflammatory mediators, including complement factors and bacterial peptides, neutrophils respond with changes in their membrane potential, intracellular pH, and cellular size. This study provides an approach to quantify these important changes simultaneously using multiparametric flow cytometry, thereby revealing a typical sequence of neutrophil activation consisting of depolarization, alkalization, and increase in cellular size. Additionally, the time resolution of the flow cytometric measurement is improved in order to allow changes that occur within seconds to be monitored, and thus to enhance the kinetic analysis of the neutrophil response. The method is appropriate for the reliable semiquantitative detection of small variations with respect to an increase, no change, and decrease in those parameters as demonstrated by the screening of various proinflammatory mediators. As a translational outlook, the findings are put into context in inflammatory conditions in vitro as well as in a clinically relevant whole blood model of endotoxemia. Taken together, the multiparametric analysis of neutrophil responsiveness regarding depolarization, alkalization, and changes in cellular size may contribute to a better understanding of neutrophils in health and disease, thus potentially yielding innovative mechanistic insights and possible novel diagnostic and/or prognostic approaches.
A Review of Capillary Pressure Control Valves in Microfluidics
Authors: Wang Shaoxi , Zhang Xiafeng , Ma Cong , Yan Sheng , Inglis David , Feng Shilun
Published: October 2021

Microfluidics offer microenvironments for reagent delivery, handling, mixing, reaction, and detection, but often demand the affiliated equipment for liquid control for these functions. As a helpful tool, the capillary pressure control valve (CPCV) has become popular to avoid using affiliated equipment. Liquid can be handled in a controlled manner by using the bubble pressure effects. In this paper, we analyze and categorize the CPCVs via three determining parameters: surface tension, contact angle, and microchannel shape. Finally, a few application scenarios and impacts of CPCV are listed, which includes how CPVC simplify automation of microfluidic networks, work with other driving modes; make extensive use of microfluidics by open channel, and sampling and delivery with controlled manners. The authors hope this review will help the development and use of the CPCV in microfluidic fields in both research and industry.
Skin Lesion Classification Based on Surface Fractal Dimensions and Statistical Color Cluster Features Using an Ensemble of Machine Learning Techniques
Authors: Moldovanu Simona , Damian Michis Felicia Anisoara , Biswas Keka C. , Culea-Florescu Anisia , Moraru Luminita
Published: October 2021

(1) Background: An approach for skin cancer recognition and classification by implementation of a novel combination of features and two classifiers, as an auxiliary diagnostic method, is proposed. (2) Methods: The predictions are made by k-nearest neighbor with a 5-fold cross validation algorithm and a neural network model to assist dermatologists in the diagnosis of cancerous skin lesions. As a main contribution, this work proposes a descriptor that combines skin surface fractal dimension and relevant color area features for skin lesion classification purposes. The surface fractal dimension is computed using a 2D generalization of Higuchi’s method. A clustering method allows for the selection of the relevant color distribution in skin lesion images by determining the average percentage of color areas within the nevi and melanoma lesion areas. In a classification stage, the Higuchi fractal dimensions (HFDs) and the color features are classified, separately, using a kNN-CV algorithm. In addition, these features are prototypes for a Radial basis function neural network (RBFNN) classifier. The efficiency of our algorithms was verified by utilizing images belonging to the 7-Point, Med-Node, and PH2 databases; (3) Results: Experimental results show that the accuracy of the proposed RBFNN model in skin cancer classification is 95.42% for 7-Point, 94.71% for Med-Node, and 94.88% for PH2, which are all significantly better than that of the kNN algorithm. (4) Conclusions: 2D Higuchi’s surface fractal features have not been previously used for skin lesion classification purpose. We used fractal features further correlated to color features to create a RBFNN classifier that provides high accuracies of classification.
Spontaneous Calcium Oscillations through Differentiation: A Calcium Imaging Analysis of Rat Cochlear Nucleus Neural Stem Cells
Authors: Voelker Johannes , Voelker Christine , Engert Jonas , Goemann Nikolas , Hagen Rudolf , Rak Kristen
Published: October 2021

Causal therapies for the auditory-pathway and inner-ear diseases are still not yet available for clinical application. Regenerative medicine approaches are discussed and examined as possible therapy options. Neural stem cells could play a role in the regeneration of the auditory pathway. In recent years, neural stem and progenitor cells have been identified in the cochlear nucleus, the second nucleus of the auditory pathway. The current investigation aimed to analyze cell maturation concerning cellular calcium activity. Cochlear nuclei from PND9 CD rats were microscopically dissected and propagated as neurospheres in free-floating cultures in stem-cell medium (Neurobasal, B27, GlutaMAX, EGF, bFGF). After 30 days, the dissociation and plating of these cells took place under withdrawal of the growth factors and the addition of retinoic acid, which induces neural cell differentiation. Calcium imaging analysis with BAPTA-1/Oregon Green was carried out at different times during the differentiation phase. In addition, the influence of different voltage-dependent calcium channels was analyzed through the targeted application of inhibitors of the L-, N-, R- and T-type calcium channels. For this purpose, comparative examinations were performed on CN NSCs, and primary CN neurons. As the cells differentiated, a significant increase in spontaneous neuronal calcium activity was demonstrated. In the differentiation stage, specific frequencies of the spontaneous calcium oscillations were measured in different regions of the individual cells. Initially, the highest frequency of spontaneous calcium oscillations was ascertainable in the maturing somata. Over time, these were overtaken by calcium oscillations in the axons and dendrites. Additionally, in the area of the growth cones, an increasing activity was determined. By inhibiting voltage-dependent calcium channels, their expression and function in the differentiation process were confirmed. A comparable pattern of maturation of these channels was found in CN NSCs and primary CN neurons. The present results show that neural stem cells of the rat cochlear nucleus differentiated not only morphologically but also functionally. Spontaneous calcium activities are of great relevance in terms of neurogenesis and integration into existing neuronal structures. These functional aspects of neurogenesis within the auditory pathway could serve as future targets for the exogenous control of neuronal regeneration.
Fluctuations in AKT and PTEN Activity Are Linked by the E3 Ubiquitin Ligase cCBL
Authors: Olazábal-Morán Manuel , Sánchez-Ortega Miriam , Martínez-Muñoz Laura , Hernández Carmen , Rodríguez Manuel S. , Mellado Mario , Carrera Ana C.
Published: October 2021

3-Poly-phosphoinositides (PIP3) regulate cell survival, division, and migration. Both PI3-kinase (phosphoinositide-3-kinase) and PTEN (phosphatase and tensin-homolog in chromosome 10) control PIP3 levels, but the mechanisms connecting PI3-kinase and PTEN are unknown. Using non-transformed cells, the activation kinetics of PTEN and of the PIP3-effector AKT were examined after the addition of growth factors. Both epidermal growth factor and serum induced the early activation of AKT and the simultaneous inactivation of PTEN (at ~5 min). This PIP3/AKT peak was followed by a general reduction in AKT activity coincident with the recovery of PTEN phosphatase activity (at ~10–15 min). Subsequent AKT peaks and troughs followed. The fluctuation in AKT activity was linked to that of PTEN; PTEN reconstitution in PTEN-null cells restored AKT fluctuations, while PTEN depletion in control cells abrogated them. The analysis of PTEN activity fluctuations after the addition of growth factors showed its inactivation at ~5 min to be simultaneous with its transient ubiquitination, which was regulated by the ubiquitin E3 ligase cCBL (casitas B-lineage lymphoma proto-oncogene). Protein-protein interaction analysis revealed cCBL to be brought into the proximity of PTEN in a PI3-kinase-dependent manner. These results reveal a mechanism for PI3-kinase/PTEN crosstalk and suggest that cCBL could be new target in strategies designed to modulate PTEN activity in cancer.
Evaluation of an Early Intervention Model for Child and Adolescent Victims of Interpersonal Violence
Authors: Calvano Claudia , Murray Elena , Bentz Lea , Bos Sascha , Reiter Kathrin , Ihme Loretta , Winter Sibylle M.
Published: October 2021

Only the minority of youth exposed to traumatic events receive mental health care, as trauma-informed clinical services are lacking or are poorly accessible. In order to bridge this gap, the Outpatient Trauma Clinic (OTC) was founded, an easily accessible early, short-time intervention, with onward referral to follow-up treatment. This report presents the OTC’s interventional approach and first outcome data. Using a retrospective naturalistic design, we analyzed trauma- and intervention-related data of the sample (n = 377, 55.4% female, mean age 10.95, SD = 4.69). Following drop-out analyses, predictors for treatment outcome were identified by logistic regression. The majority (81.9%) was suffering from posttraumatic stress disorder (PTSD) or adjustment disorders. Around one forth dropped out of treatment; these cases showed higher avoidance symptoms at presentation. In 91%, psychological symptoms improved. Experience of multiple traumatic events was the strongest predictor for poor treatment outcome (B = −0.823, SE = 0.313, OR = 0.439, 95% CI 0.238–0.811). Around two thirds were connected to follow-up treatment. The OTC realized a high retention rate, initial improvement of symptoms and referral to subsequent longer-term psychotherapeutic treatment in the majority. Further dissemination of comparable early intervention models is needed, in order to improve mental health care for this vulnerable group.
Annealing Effect on the Contact Angle, Surface Energy, Electric Property, and Nanomechanical Characteristics of Co40Fe40W20 Thin Films
Authors: Liu Wen-Jen , Chang Yung-Huang , Fern Chi-Lon , Chen Yuan-Tsung , Jhou Tian-Yi , Chiu Po-Chun , Lin Shih-Hung , Lin Ko-Wei , Wu Te-Ho
Published: October 2021

This study investigated Co40Fe40W20 single-layer thin films according to their corresponding structure, grain size, contact angle, and surface energy characteristics. Co40Fe40W20 alloy thin films of different thicknesses, ranging from 10 to 50 nm, were sputtered on Si(100) substrates by DC magnetron sputtering. The thin films were annealed under three conditions: as-deposited, 250 °C, and 350 °C temperatures, respectively. The Scherrer equation was applied to calculate the grain size of Co40Fe40W20 thin films. The results show that the grain size of CoFe(110) increased simultaneously with the increase of post-annealing temperature, suggesting that the crystallinity of Co40Fe40W20 thin films increased with the post-annealing temperature. Moreover, the contact angles of all Co40Fe40W20 thin films were all less than 90°, suggesting that Co40Fe40W20 thin films show changes in the direction of higher hydrophilicity. However, we found that their contact angles decreased as the grain size of CoFe increased. Finally, the Young equation was applied to calculate the surface energy of Co40Fe40W20 thin films. After post-annealing, the surface energy of Co40Fe40W20 thin films increased with the rising post-annealing temperature. This is the highest value of surface energy observed for 350 °C. In addition, the surface energy increased as the contact angle of Co40Fe40W20 thin films decreased. The high surface energy means stronger adhesion, allowing the formation of multilayer thin films with magnetic tunneling junctions (MTJs). The sheet resistance of the as-deposited and thinner CoFeW films is larger than annealed and thicker CoFeW films. When the thickness is from 10 nm to 50 nm, the hardness and Young’s modulus of the CoFeW film also show a saturation trend.
One-Step Multi-Doping Process for Producing Effective Zinc Oxide Nanofibers to Remove Industrial Pollutants Using Sunlight
Authors: Saber Osama , Shaalan Nagih M. , Ahmed Faheem , Kumar Shalendra , Alshoaibi Adil
Published: October 2021

Doping processes for optical materials are one of the driving forces for developing efficient and clean technologies for decontamination of aquatic effluents through lowering their band gap energy to become effective in sunlight. The current study has used a non-conventional technique for doping zinc oxide by multi metals, non-metals and organic dyes through a one-step process. In this trend, Zn-Al nanolayered structures have been used as hosts for building host–guest interactions. Organic dyes that have inorganic species of iron, nitrogen and sulfur have been used as guests in the intercalation reactions of Zn-Al layered double hydroxides. By intercalating green dyes, organic–inorganic nanohybrids were formed as nanolayered structures with expanding interlayered spacing to 2.1 nm. By changing the concentration of green dyes and thermal treatment, series of nanofibers and nanoplates of zinc oxides were formed and doped by aluminum, iron and sulfur in addition to colored species. The optical properties of the multi-doped zinc oxide indicated that it became suitable for solar applications because its band gap energy decreased from 3.30 eV to 2.80 eV. The experimental and kinetic results of the multi-doped zinc oxide concluded that the colored pollutants were effectively removed during 50 min of sunlight irradiation.
Spectroscopic and Structural Properties of β-Tricalcium Phosphates Ca9RE(PO4)7 (RE = Nd, Gd, Dy)
Authors: Paterlini Veronica , El Khouri Asmaa , Bettinelli Marco , Trucchi Daniele Maria , Capitelli Francesco
Published: October 2021

Rare-earth-based Ca9RE(PO4)7 (RE = Nd, Gd, Dy) materials were synthesized by solid-state reaction at T = 1200 °C. The obtained tricalcium phosphate (TCP) materials are efficient light emitters due to the presence of RE3+ ions, although these ions are present at high concentrations. Moreover, in these host structures, these ions can be used as optical probes to study their local environments. Thus, photoluminescence (PL) emission spectra of the powder samples clearly indicated, for Dy3+ and Gd3+ ions, the presence of the RE3+ ion in low-symmetry sites with some local structural disorder, and the spectra show the presence of vibrational features (in the case of Gd3+). For the Nd3+ phase, emission bands are present around 900, 1050, and 1330 nm, originating from the 4F3/2 level. In general, these RE-TCP samples are interesting luminescent materials in the visible (Dy), UV (Gd), and NIR (Nd) regions, due to weak concentration quenching even for high concentrations of the emitting ion.
Overlooked Species Diversity and Distribution in the Antarctic Mite Genus Stereotydeus
Authors: Brunetti Claudia , Siepel Henk , Convey Peter , Fanciulli Pietro Paolo , Nardi Francesco , Carapelli Antonio
Published: October 2021

In the harsh Antarctic terrestrial ecosystems, invertebrates are currently confined to sparse and restricted ice free areas, where they have survived on multi-million-year timescales in refugia. The limited dispersal abilities of these invertebrate species, their specific habitat requirements, and the presence of geographical barriers can drastically reduce gene flow between populations, resulting in high genetic differentiation. On continental Antarctica, mites are one of the most diverse invertebrate groups. Recently, two new species of the free living prostigmatid mite genus Stereotydeus Berlese, 1901 were discovered, bringing the number of Antarctic and sub-Antarctic species of this genus up to 15, of which 7 occur along the coast of Victoria Land and in the Transantarctic Mountains. To examine the biodiversity of Stereotydeus spp., the present study combines phylogenetic, morphological and population genetic data of specimens collected from nine localities in Victoria Land. Genetically distinct intraspecific groups are spatially isolated in northern Victoria Land, while, for other species, the genetic haplogroups more often occur sympatrically in southern Victoria Land. We provide a new distribution map for the Stereotydeus species of Victoria Land, which will assist future decisions in matters of the protection and conservation of the unique Antarctic terrestrial fauna.
Association of Cervical and Lumbar Paraspinal Muscle Composition Using Texture Analysis of MR-Based Proton Density Fat Fraction Maps
Authors: Burian Egon , Becherucci Edoardo A. , Junker Daniela , Sollmann Nico , Greve Tobias , Hauner Hans , Zimmer Claus , Kirschke Jan S. , Karampinos Dimitrios C. , Subburaj Karupppasamy , Baum Thomas , Dieckmeyer Michael
Published: October 2021

In this study, the associations of cervical and lumbar paraspinal musculature based on a texture analysis of proton density fat fraction (PDFF) maps were investigated to identify gender- and anatomical location-specific structural patterns. Seventy-nine volunteers (25 men, 54 women) participated in the present study (mean age ± standard deviation: men: 43.7 ± 24.6 years; women: 37.1 ± 14.0 years). Using manual segmentations of the PDFF maps, texture analysis was performed and texture features were extracted. A significant difference in the mean PDFF between men and women was observed in the erector spinae muscle (p < 0.0001), whereas the mean PDFF did not significantly differ in the cervical musculature and the psoas muscle (p > 0.05 each). Among others, Variance(global) and Kurtosis(global) showed significantly higher values in men than in women in all included muscle groups (p < 0.001). Not only the mean PDFF values (p < 0.001) but also Variance(global) (p < 0.001), Energy (p < 0.001), Entropy (p = 0.01), Homogeneity (p < 0.001), and Correlation (p = 0.037) differed significantly between the three muscle compartments. The cervical and lumbar paraspinal musculature composition seems to be gender-specific and has anatomical location-specific structural patterns.