bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Alloying Elements on the Sharpness Retention of Knife Blades Made of High Carbon Martensitic Stainless Steels
Authors: Wu Dong , Zhang Qinyi , Liu Wei
Published: March 2022

Blades usually become blunt as the blade tip suffers wear during cutting, and improving the sharpness retention of steel blades has become an attractive prospect in various industries. In this study, blades were fabricated from commercial high carbon martensitic stainless steels (154CM, 440C and N690) with different contents of alloying elements. 154CM with higher Mo content demonstrated superior capability in sharpness retention to 440C and N690, although these steels exhibit similar chemical composition, carbide phases, microstructure and HRC hardness. Further investigations via SEM and nanoindentation indicated that the faster deterioration of sharpness in 440C and N690 may result from the fatigue peeling of the carbides, which was aggravated by the modulus mismatch between carbide particles and the martensitic steel matrix.
Improving Mechanical Properties of a Forged High-Manganese Alloy by Regulating Carbon Content and Carbide Precipitation
Authors: Pan Zhizhou , Liu Tao , Li Jiang , Wang Lei , Zhang Tianyu , Wang Jian , Tao Qing
Published: March 2022

The effect of different heat treatment processes (as-cast, annealing, forging, and annealing after forging) on the microstructure transition and mechanical property evolution of Fe50Mn30Co10Cr10 alloys with different carbon contents (0, 0.2, 0.5 wt.%) was investigated, and a potential strengthening–toughening mechanism was revealed. With 0.5 wt.% carbon added, the interstitial carbon atoms provided a great deal of strength and the highest hardness was obtained. Meanwhile, the high carbon content generated a large amount of stacking fault energy and inhibited the transition of a face-centered cubic (FCC) to a hexagonal close-packed phase (HCP); as such, the TRIP and TWIP effects were induced during deformation and a favorable ductility with the largest elongation to fracture (of 141%) was achieved. The forged-annealed specimen with 0.2 wt.% carbon obtained favorable comprehensive mechanical properties, with an ultimate tensile strength of 795 MPa and an elongation of 104%. After forging, the grains were refined and several dislocations were generated; as such, the yield strength was greatly improved. With subsequent annealing, a good phase distribution of FCC and HCP was achieved, inducing the TRIP and TWIP effects during deformation and producing favorable ductility.
Plasma Tsukushi Concentration Is Associated with High Levels of Insulin and FGF21 and Low Level of Total Cholesterol in a General Population without Medication
Authors: Furuhashi Masato , Higashiura Yukimura , Sakai Akiko , Koyama Masayuki , Tanaka Marenao , Saitoh Shigeyuki , Shimamoto Kazuaki , Ohnishi Hirofumi
Published: March 2022

Tsukushi (TSK) is a member of the small leucine-rich proteoglycan family that controls developmental processes and organogenesis. TSK was also identified as a new hepatokine, which is mainly expressed in the liver, and is secreted by hepatocytes, to regulate energy and glycolipid metabolism in response to nonalcoholic fatty liver disease. However, the role of plasma TSK, especially its role in the general population, has not been fully addressed. We investigated the associations between plasma TSK concentration and several metabolic markers, including fibroblast growth factor 21 (FGF21), a hepatokine, and adiponectin, an adipokine, in 253 subjects (men/women: 114/139) with no medication in the Tanno–Sobetsu Study, which employed a population-based cohort. There was no significant sex difference in plasma TSK concentration, and the level was positively correlated with the fatty liver index (FLI) (r = 0.131, p = 0.038), levels of insulin (r = 0.295, p < 0.001) and levels of FGF21 (r = 0.290, p < 0.001), and was negatively correlated with the total cholesterol level (r = −0.124, p = 0.049). There was no significant correlation between the TSK level and body mass index, waist circumference, adiponectin, high-density lipoprotein cholesterol or total bile acids. The multivariable regression analysis showed that high levels of insulin and FGF21 and a low level of total cholesterol were independent determinants of plasma TSK concentration, after adjustment for age, sex and FLI. In conclusion, plasma TSK concentration is independently associated with high levels of insulin and FGF21, a hepatokine, and a low level of total cholesterol, but not with adiposity and adiponectin, in a general population of subjects who have not taken any medications.
Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device
Authors: Phiphattanaphiphop Chalinee , Leksakul Komgrit , Wanta Thananut , Khamlor Trisadee , Phattanakun Rungrueang
Published: March 2022

This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were discarded, so that X-sperms can be isolated and commercially exploited for fertilization demands of female cattle in dairy industry. Therefore, the research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The experimental design was divided into the following: Model 1, the microfluid system for sorting positive magnetic beads, which yielded 100% separation; Model 2, the sorting of monoclonal antibody-conjugated magnetic beads in the fluid system, yielding 98.84% microcirculation; Model 3, the sorting of monoclonal antibody-conjugated magnetic beads with sperm in the microfluid system, yielding 80.12% microcirculation. Moreover, the fabrication microfluidic system had thin film electrodes created via UV lithography and MWCNTs electrode structure capable of erecting an electrode wall 1500 µm above the floor with a flow channel width of only 100 µm. The system was tested using a constant flow rate of 2 µL/min and X-/Y-sperm were separated using carbon nanotube electrodes at 2.5 V. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency.
Review of Electrothermal Micromirrors
Authors: Tang Yue , Li Jianhua , Xu Lixin , Lee Jeong-Bong , Xie Huikai
Published: March 2022

Electrothermal micromirrors have become an important type of micromirrors due to their large angular scanning range and large linear motion. Typically, electrothermal micromirrors do not have a torsional bar, so they can easily generate linear motion. In this paper, electrothermal micromirrors based on different thermal actuators are reviewed, and also the mechanisms of those actuators are analyzed, including U-shape, chevron, thermo-pneumatic, thermo-capillary and thermal bimorph-based actuation. Special attention is given to bimorph based-electrothermal micromirrors due to their versatility in tip-tilt-piston motion. The exemplified applications of each type of electrothermal micromirrors are also presented. Moreover, electrothermal micromirrors integrated with electromagnetic or electrostatic actuators are introduced.
Mechanical Characterization and In Vitro Assay of Biocompatible Titanium Alloys
Authors: Baltatu Iustinian , Sandu Andrei Victor , Vlad Maria Daniela , Spataru Mihaela Claudia , Vizureanu Petrica , Baltatu Madalina Simona
Published: March 2022

Metals that come into contact with the body can cause reactions in the body, so biomaterials must be tested to avoid side effects. Mo, Zr, and Ta are non-toxic elements; alloyed with titanium, they have very good biocompatibility properties and mechanical properties. The paper aims to study an original Ti20Mo7ZrxTa system (5, 10, 15 wt %) from a mechanical and in vitro biocompatibility point of view. Alloys were examined by optical microstructure, tensile strength, fractographic analysis, and in vitro assay. The obtained results indicate very good mechanical and biological properties, recommending them for future orthopedic medical applications.
Design and Computational Analyses of Nature Inspired Unmanned Amphibious Vehicle for Deep Sea Mining
Authors: Raja Vijayanandh , Solaiappan Senthil Kumar , Kumar Lokeshkumar , Marimuthu Arishwaran , Gnanasekaran Raj Kumar , Choi Yosoon
Published: March 2022

This paper presents the design calculations, implementations, and multi-engineering based computational constructions of an unmanned amphibious vehicle (UAmV) which efficiently travels underwater to detect and collect deep-sea minerals for investigations, as well as creative usage purposes. The UAmV is expected to operate at a 300 m depth from the water surface. The UAmV is deployed above the water surface near to the approximate target location and swims underwater, checking the presence of various mining, then extracts them using a unique mechanism and stores them in an inimitable fuselage location. Since this proposed UAmV survives in deep-sea regions, the design construction of this UAmV is inspired by hydrodynamic efficient design-based fish, i.e., Rhinaancylostoma. Additionally, standard analytical approaches are followed and, subsequently, the inimitable components such as wing, stabilizers, propellers, and mining storage focused fuselage are calculated. The computational analyses such as hydrodynamic investigations and vibrational investigations were carried out with the help of ANSYS Workbench. The hydrodynamic pressures at various deployment regions were estimated and thereafter the vibrational outcomes of UAmVs were captured for various lightweight materials. The computed outcomes were imposed in the analytical approach and thereby the electrical energy generations by the UAmV’s components were calculated. Finally, the hydrodynamic efficient design and best material were picked, which provided a path to further works on the execution of the focused mission. Based on the low drag generating design profile and high electrical energy induction factors, the optimizations were executed on this work, and thus the needful, as well as suitable UAmV, was finalized for targeted real-time applications.
Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing
Authors: Liu Yang , Wu Ning , Geng Lihua , Yue Yang , Zhang Quanbin , Wang Jing
Published: March 2022

Nowadays, natural polysaccharides-based hydrogels have achieved promising results as dressings to promote skin healing. In the present study, we prepared a novel hydrogel nanocomposite with poly(vinyl alcohol) (PVA) and sulfated heterosaccharide (UF), named UPH. The SEM results showed that the UPH had dense porous structures with a high porosity and a specific surface area. The UPH had a good swelling property, which can effectively adsorb exudate and keep the wound moist. The in vitro experiments results showed that the UPH was non-cytotoxic and could regulate the inflammatory response and promote the migration of fibroblasts significantly. The phenotypic, histochemistry, and Western blot analyses showed UPH treatment accelerated the wound healing and recovery of skin tissue at wound sites in a C57BL/6 mouse model. Furthermore, the UPH could promote the inflammation process to onset earlier and last shorter than that in a normal process. Given its migration-promoting ability and physicochemical properties, the UPH may provide an effective application for the treatment and management of skin wounds.
Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides’ and Amino Acids’ Micro- and Nanostructures
Authors: Handelman Amir
Published: March 2022

In recent years, self-assembled peptides’ and amino acids’ (SAPA) micro- and nanostructures have gained much research interest. Here, description of how SAPA architectures can be characterized using polarization-based optical measurement methods is provided. The measurement methods discussed include: polarized Raman spectroscopy, polarized imaging microscopy, birefringence imaging, and fluorescence polarization. An example of linear polarized waveguiding in an amino acid Histidine microstructure is discussed. The implementation of a polarization-based measurement method for monitoring peptide self-assembly processes and for deriving molecular orientation of peptides is also described.
High Performance Liquid Chromatography (HPLC) with Fluorescence Detection for Quantification of Steroids in Clinical, Pharmaceutical, and Environmental Samples: A Review
Authors: Hameedat Fatima , Hawamdeh Sahar , Alnabulsi Soraya , Zayed Aref
Published: March 2022

Steroids are compounds widely available in nature and synthesized for therapeutic and medical purposes. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with the sensitivity, selectivity, simplicity, and cost-efficiency of fluorescence, make HPLC coupled to fluorescence detection (HPLC-FLD) an ideal tool for routine measurement and detection of steroids. In this review, we covered HPLC-FLD methods reported in the literature for the steroids quantification in clinical, pharmaceutical, and environmental applications, focusing on the various approaches of fluorescent derivatization. The aspects related to analytical methodology including sample preparation, derivatization reagents, and chromatographic conditions will be discussed.
Influence of Salinity Stress on Color Parameters, Leaf Pigmentation, Polyphenol and Flavonoid Contents, and Antioxidant Activity of Amaranthus lividus Leafy Vegetables
Authors: Hossain Md. Nazmul , Sarker Umakanta , Raihan Md. Sharif , Al-Huqail Asma A. , Siddiqui Manzer H. , Oba Shinya
Published: March 2022

This is the first attempt to evaluate the impact of four salinity levels on the color parameters, pigments, polyphenols, flavonoids, and antioxidant capacities of four promising A. lividus genotypes. The color parameters, such as the yellowness/blueness (b*) and the chroma (C*); the antioxidant components, such as the polyphenols and flavonoids; and the antioxidant capacities of the leaves were remarkably increased by 39, 1, 5, 10 and 43%, respectively, at 50 mM of NaCl, and by 55, 5, 60, 34, 58 and 82%, respectively, at 100 mM NaCl concentrations. The green tower and SA6 genotypes were identified as tolerant varieties. The total phenolic content (TPC) and the total flavonoid content (TFC) played vital roles in scavenging reactive oxygen species (ROS), and they would be beneficial for the human diet and would serve as good antioxidants for the prevention of aging, and they are also essential to human health. A correlation study revealed the strong antioxidant capacities of the pigments and antioxidant components that were studied. It was revealed that A. lividus could tolerate a certain level of salinity stress without compromising the antioxidant quality of the final product. Taken together, our results suggest that A. lividus could be a promising alternative crop for farmers, especially in saline-prone areas in the tropical and subtropical regions.
A Method for Prediction of Ultrasonic Detectability of Interface Gap Defects on TC4 Diffusion-Bonded Joints
Authors: Teng Lichen , Zhou Zhenggan
Published: March 2022

An analysis method for the detectability of defects on the TC4 (Ti-6Al-4V) diffusion bonding interface was proposed in this study. First, a semi-analytical model of the liquid–solid coupling acoustic field with attenuation characteristics was constructed. Based on this, a method for the selection of transducer parameters was investigated for effective focus on the diffusion bonding interface. Second, according to the characteristics of defects on the diffusion bonding interface, an acoustic response model for diffusion bonding defects was established based on Kirchhoff approximation. The detectability of defects on the diffusion bonding interface was analyzed using transducers of different frequencies with different diffusion bonding interface gaps. Finally, an experiment was conducted to verify the reliability of the simulation. The analysis method proposed shows the advantages in the selection of suitable parameters for detecting specific diffusion bonding interface gaps, providing theoretical predictions of the detectability of diffusion bonding interface defects.
Ribosome-Associated ncRNAs (rancRNAs) Adjust Translation and Shape Proteomes
Authors: Pecoraro Valentina , Rosina Alessia , Polacek Norbert
Published: March 2022

The regulation of protein synthesis is of extreme importance for cell survival in challenging environmental conditions. Modulating gene expression at the level of translation allows a swift and low-energy-cost response to external stimuli. In the last decade, an emerging class of regulatory ncRNAs, namely ribosome-associated non-coding RNAs (rancRNAs), has been discovered. These rancRNAs have proven to be efficient players in the regulation of translation as a first wave of stress adaptation by directly targeting the ribosome, the central enzyme of protein production. This underlying principle appears to be highly conserved, since rancRNAs are present in all three domains of life. Here, we review the major findings and mechanistic peculiarities of rancRNAs, a class of transcripts that is providing new and broader perspectives on the complexity of the ribosome and translation regulation.
Neuroprotective Effects of Nicotinamide (Vitamin B3) on Neurodegeneration in Diabetic Rat Retinas
Authors: Jung Kyoung In , Han Jeong-Sun , Park Chan Kee
Published: March 2022

The loss of inner retinal neurons is an initial event in diabetic retinopathy. In diabetic retinas, oxidative stress is increased, which could lead to increased oxidative DNA damage. Nicotinamide is a precursor to nicotinamide adenine dinucleotide, which contributes to the DNA damage response. We investigated whether nicotinamide plays a neuroprotective role in diabetic retinal neurodegeneration in terms of DNA repair. Male Sprague Dawley rats with streptozotocin-induced diabetes were orally administered nicotinamide (500 mg/kg/day) for 4 or 12 weeks. Oxidative stress exhibited by dihydroethidium was upregulated at 4 and 12 weeks after onset of diabetes, and nicotinamide treatment reduced oxidative stress at 4 weeks after induction of diabetes. Oxidative DNA damage measured by 8-hydroxy-2′-deoxyguanosine (8-OHdG) increased at 4 and 12 weeks after induction of diabetes and decreased following nicotinamide treatment. The elevated expression of glial fibrillary acidic protein (GFAP) induced by diabetes was attenuated by nicotinamide treatment. In Western blot analysis, the increased expression of cleaved PARP-1 in diabetes was attenuated by nicotinamide treatment at 12 weeks after induction of diabetes. The diabetes-induced apoptosis of inner retinal cells detected by the TUNEL assay was reduced by nicotinamide treatment. In conclusion, nicotinamide attenuated retinal neurodegeneration in diabetes, probably by reducing oxidative DNA damage and supporting DNA repair.
The Suitability of Questionnaires for Exploring Relations of Dietary Behavior and Tooth Wear
Authors: Schlenz Maximiliane Amelie , Schlenz Moritz Benedikt , Wöstmann Bernd , Jungert Alexandra , Glatt Anna Sophia , Ganss Carolina
Published: March 2022

Tooth wear is a relevant oral health problem, especially at a young age. Although ongoing acid exposures may contribute to tooth wear, the role of acidic dietary components in this context remains unclear. To date, in tooth wear studies, dietary behavior has been assessed using traditional questionnaires, but the suitability of this approach has not been investigated so far. In our longitudinal study, we followed 91 participants (21.0 ± 2.2 years) over a period of 1 year (373 ± 19 days) and monitored tooth wear with an intraoral scanner. At baseline (T0) and at the end (T1), we assessed dietary behavior with questionnaires asking about the consumption frequencies of acidic dietary components and the acid taste preferences. Complete data were available from 80 subjects. The consumption frequencies of T0 and T1 correlated weakly to moderately. Taste preferences seem to be a more consistent measure, but there was predominantly no significant correlation with the corresponding consumption frequencies. None of the dietary parameters showed a significant relation with tooth wear. The suitability of dietary questionnaires to assess tooth-relevant dietary behavior seems to be limited.
Calcifediol (25OH Vitamin D3) Deficiency: A Risk Factor from Early to Old Age
Authors: Bouillon Roger , Antonio Leen , Olarte Oscar Rosero
Published: March 2022

Vitamin D deficiency is the main cause of nutritional rickets in children and osteomalacia in adults. There is consensus that nutritional access to vitamin D can be estimated by measuring serum concentrations of 25OHD and vitamin D deficiency can thus be considered as calcifediol deficiency. However, the threshold for vitamin D/calcifediol sufficiency remains a matter of debate. Vitamin D/calcifediol deficiency has been associated with musculoskeletal effects but also multiple adverse extra-skeletal consequences. If these consequences improve or if they can be treated with vitamin D supplementation is still unclear. Observational studies suggest a higher infection risk in people with low calcifediol levels. There is also a consistent association between serum calcifediol and cardiovascular events and deaths, but large-scale, long-term intervention studies did not show any benefit on cardiovascular outcomes from supplementation, at least not in subjects without clear vitamin D deficiency. Cancer risk also did not change with vitamin D treatment, although there are some data that higher serum calcifediol is associated with longer survival in cancer patients. In pregnant women, vitamin D supplementation decreases the risk of pre-eclampsia, gestational diabetes mellitus, and low birth weight. Although preclinical studies showed that the vitamin D endocrine system plays a role in certain neural cells as well as brain structure and function, there is no evidence to support a beneficial effect of vitamin D in neurodegenerative diseases. Vitamin D supplementation may marginally affect overall mortality risk especially in elderly subjects with low serum calcifediol concentrations.
Track Reconstruction in a High-Density Environment with ALICE
Authors: Arslandok Mesut , Hellbär Ernst , Ivanov Marian , Münzer Robert Helmut , Wiechula Jens
Published: March 2022

ALICE is the dedicated heavy-ion experiment at the CERN Large Hadron Collider (LHC). Its main tracking and particle-identification detector is a large volume Time Projection Chamber (TPC). The TPC has been designed to perform well in the high-track density environment created in high-energy heavy-ion collisions. In this proceeding, we describe the track reconstruction procedure in ALICE. In particular, we focus on the two main challenges that were faced during the Run 2 data-taking period (2015–2018) of the LHC, which were the baseline fluctuations and the local space charge distortions in the TPC. We present the corresponding solutions in detail and describe the software tools that allowed us to circumvent these challenges.
Toxoplasma gondii Infection Is Associated with Low Birth Weight: Findings from an Observational Study among Rural Bangladeshi Women
Authors: Parvin Irin , Das Sumon Kumar , Ahmed Shahnawaz , Rahman Aminur , Shahid Abu Sadat Mohammad Sayeem Bin , Shahrin Lubaba , Afroze Farzana , Ackhter Mst. Mahmuda , Alam Tahmina , Jahan Yasmin , Palit Parag , Sarker Mohammad Habibur Rahman , Das Jui , Hoque Mohammad Enamul , Magalhães Ricardo J. Soares , Mamun Abdullah Al , Faruque Abu Syed Golam , Ahmed Tahmeed , Chisti Mohammod Jobayer
Published: March 2022

Gestational Toxoplasma gondii (T. gondii) infection may cause substantial adverse effects on developing fetuses, newborns and also mothers. This study aims to estimate the seroprevalence of T. gondii among rural Bangladeshi pregnant women and determine the risk of a low birth weight (LBW). We followed a longitudinal design where 208 pregnant women were followed until the birth of their infants. Levels of IgG and IgM of T. gondii were assessed using chemiluminescent immunoassay. Modified Poisson regression was used to estimate crude and adjusted associations and multiple regression analysis was performed to understand the confounding and modifying effects of the variables. Thirty-nine (19%) children were born with LBW, among whom 15 (39%) mothers were positive for T. gondii IgG during pregnancy. After adjusting for several confounders and modifiers, pregnant women with T. gondii IgG or IgM seropositivity were significantly associated with LBW of infants (aRR: 2.00, 95% CI: 1.17–3.42). The strength of this association increased after adjusting for maternal education (aRR: 4.88, 95% CI: 1.74–13.69). The final model had an AROC of 0.84 with a sensitivity of 36% and specificity of 97%. Although causality is yet to be established, the study observed an association between T. gondii infection during pregnancy among rural Bangladeshi women and LBW of newborns.
Molecular Signaling Mechanisms for the Antidepressant Effects of NLX-101, a Selective Cortical 5-HT1A Receptor Biased Agonist
Authors: Cabanu Sharon , Pilar-Cuéllar Fuencisla , Zubakina Paula , Florensa-Zanuy Eva , Senserrich Júlia , Newman-Tancredi Adrian , Adell Albert
Published: March 2022

Depression is the most prevalent of the mental illnesses and serotonin (5-hydroxytryptamine, 5-HT) is considered to be the major neurotransmitter involved in its etiology and treatment. In this context, 5-HT1A receptors have attracted interest as targets for therapeutic intervention. Notably the activation of presynaptic 5-HT1A autoreceptors delays antidepressant effects whereas the stimulation of postsynaptic 5-HT1A heteroreceptors is needed for an antidepressant action. NLX-101 (also known as F15599) is a selective biased agonist which exhibits preferred activation of cortical over brain stem 5-HT1A receptors. Here, we used behavioral, neurochemical and molecular methods to examine the antidepressant-like effects in rats of a single dose of NLX-101 (0.16 mg/kg, i.p.). NLX-101 reduced immobility in the forced swim test when measured 30 min but not 24 h after drug administration. NLX-101 increased extracellular concentrations of glutamate and dopamine in the medial prefrontal cortex, but no changes were detected in the efflux of noradrenaline or 5-HT. NLX-101 also produced an increase in the activation of pmTOR, pERK1/2 and pAkt, and the expression of PSD95 and GluA1, which may contribute to its rapid antidepressant action.
In Vitro Biological Evaluation of Aprepitant Based 177Lu-Radioconjugates
Authors: Halik Paweł K. , Koźmiński Przemysław , Matalińska Joanna , Lipiński Piotr F. J. , Misicka Aleksandra , Gniazdowska Ewa
Published: March 2022

Currently, the search for promising NK1R-positive tumor-targeting radiopharmaceuticals based on the structure of small molecular antagonists of neurokinin-1 receptor can be observed. Following this trend, we continued our evaluation of aprepitant-based 177Lu-radioconjugates in terms of future oncological applications. For this purpose, three novel aprepitant homologues were synthesized to broaden the previously obtained derivative portfolio, functionalized with the DOTA chelator and labeled with 68Ga and 177Lu. The newly evaluated radioconjugates showed the intended significant increase in lipophilicity compared to the previous ones, while maintaining stability in the human serum. Then, in a receptor binding study to the human NK1 receptor, we compared the two series of 177Lu-radioconjugates of aprepitant with each other and with the reference Substance P derivative currently used in glioblastoma therapy, clearly indicating the high affinity and better binding capacity of the novel radioconjugates. The in vitro experimental results included in the presented study, supported by labeling optimization, radioconjugate characterization and docking modeling of new aprepitant-derived radioagents, confirm our assumptions about the usefulness of aprepitant as a NK1R targeting vector and point out the perspectives for the forthcoming first in vivo trials.
Advancing Regenerative Cellular Therapies in Non-Scarring Alopecia
Authors: Anudeep Talagavadi Channaiah , Jeyaraman Madhan , Muthu Sathish , Rajendran Ramya Lakshmi , Gangadaran Prakash , Mishra Prabhu Chandra , Sharma Shilpa , Jha Saurabh Kumar , Ahn Byeong-Cheol
Published: March 2022

Alopecia or baldness is a common diagnosis in clinical practice. Alopecia can be scarring or non-scarring, diffuse or patchy. The most prevalent type of alopecia is non-scarring alopecia, with the majority of cases being androgenetic alopecia (AGA) or alopecia areata (AA). AGA is traditionally treated with minoxidil and finasteride, while AA is treated with immune modulators; however, both treatments have significant downsides. These drawbacks compel us to explore regenerative therapies that are relatively devoid of adverse effects. A thorough literature review was conducted to explore the existing proven and experimental regenerative treatment modalities in non-scarring alopecia. Multiple treatment options compelled us to classify them into growth factor-rich and stem cell-rich. The growth factor-rich group included platelet-rich plasma, stem cell-conditioned medium, exosomes and placental extract whereas adult stem cells (adipose-derived stem cell-nano fat and stromal vascular fraction; bone marrow stem cell and hair follicle stem cells) and perinatal stem cells (umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs), Wharton jelly-derived MSCs (WJ-MSCs), amniotic fluid-derived MSCs (AF-MSCs), and placental MSCs) were grouped into the stem cell-rich group. Because of its regenerative and proliferative capabilities, MSC lies at the heart of regenerative cellular treatment for hair restoration. A literature review revealed that both adult and perinatal MSCs are successful as a mesotherapy for hair regrowth. However, there is a lack of standardization in terms of preparation, dose, and route of administration. To better understand the source and mode of action of regenerative cellular therapies in hair restoration, we have proposed the “À La Mode Classification”. In addition, available evidence-based cellular treatments for hair regrowth have been thoroughly described.
Study of the Response Surface in the Photocatalytic Degradation of Acetaminophen Using TiO2
Authors: Marizcal-Barba Adriana , Sanchez-Burgos Jorge Alberto , Zamora-Gasga Victor , Perez Larios Alejandro
Published: March 2022

An effective way to obtain the optimal parameters of a process or experiment is the response surface method. Using the Box–Behnken design further decreases the number of experiments needed to obtain sufficient data to obtain a reliable equation. From the equation, it is possible to predict the behavior of the response with respect to the combination of variables involved. In this study we evaluated the photocatalytic activity of the synthesized TiO2 for the degradation of acetaminophen, a frequently used and uncontrolled drug that has been detected with increasing frequency in wastewater effluents. The variables used for this study were pH, contaminant concentration (acetaminophen) and catalyst dose. We found, with a 95% confidence level, that 99% of the contaminant can be degraded to pH 10, contaminant to 35 mg/L and a catalyst dose of 0.15 g TiO2.
O-Band Frequency-Tunable (10–22 GHz) Ultra-Low Timing-Jitter (<12-fs) Regenerative Mode-Locked Laser
Authors: Qi Hefei , Zhang Zhihao , Lu Dan , Zhang Ruikang , Zhao Lingjuan
Published: March 2022

A frequency-tunable and low-timing-jitter O-band regenerative mode-locked laser (RMLL) using an optoelectronic oscillation configuration and electric-controlled yttrium iron garnet (YIG) bandpass filter is proposed and demonstrated. In this scheme, an O-band semiconductor optical amplifier (SOA) is used as the gain medium of the RMLL to realize a dispersion-management-free operation during frequency tuning. With a polarization-maintaining fiber loop of 300 m, we produced a robust frequency-tunable RMLL with a pulse width below 16 ps, phase noises below −123 dBc/Hz at a 10-kHz frequency offset from the carrier frequency, and timing jitter less than 12 fs (integrated in 1-kHz to 1-MHz range) in a frequency tuning range between 10 GHz and 22 GHz.
Assessment of Phenotypic Diversity in the USDA Collection of Quinoa Links Genotypic Adaptation to Germplasm Origin
Authors: Hafeez Muhammad Bilal , Iqbal Shahid , Li Yuanyuan , Saddiq Muhammad Sohail , Basra Shahzad M. A. , Zhang Hui , Zahra Noreen , Akram Muhammad Z. , Bertero Daniel , Curti Ramiro N.
Published: March 2022

Quinoa’s germplasm evaluation is the first step towards determining its suitability under new environmental conditions. The aim of this study was to introduce suitable germplasm to the lowland areas of the Faisalabad Plain that could then be used to introduce quinoa more effectively to that region. A set of 117 quinoa genotypes belonging to the USDA quinoa collection was evaluated for 11 phenotypic quantitative traits (grain yield (Y), its biological and numerical components plus phenological variables) in a RCBD during two consecutive growing seasons at the University of Agriculture, Faisalabad, Pakistan under mid-autumn sowings. Genotypic performance changed across the years, however most phenotypic traits showed high heritability, from 0.75 for Harvest Index (HI) to 0.97 for aerial biomass (B) and Y. Ordination and cluster analyses differentiated four groups dominated by genotypes from: Peru and the Bolivian Highlands (G1); the Bolivian Highlands (G2); the Ballón collection (regarded as a cross between Bolivian and Sea Level (Chilean) genotypes) plus Bolivian Highlands (G3); and Ballón plus Sea Level (G4), this latter group being the most differentiated one. This genetic structure shared similarities with previous groups identified using SSR markers and G×E data from an international quinoa test. G4 genotypes showed the highest Y associated with higher B and seed numbers (SN), while HI made a significant contribution to yield determination in G2 and seed weight (SW) in G3. G1 and G2 showed the lowest Y associated with a lower B and SN. Moreover, SW showed a strongly negative association with SN in G2. Accordingly, G4 followed by G3 are better suited to the lowland areas of Faisalabad plain and the physiological traits underlying yield determination among genotypic groups should be considered in future breeding programs.
Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS
Authors: Aboushanab Saied A. , Shevyrin Vadim A. , Slesarev Grigory P. , Melekhin Vsevolod V. , Shcheglova Anna V. , Makeev Oleg G. , Kovaleva Elena G. , Kim Ki Hyun
Published: March 2022

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries.
Polypropylene Random Copolymer Based Composite Used for Fused Filament Fabrication: Printability and Properties
Authors: Zhang Zhiyao , Gao Xueqin
Published: March 2022

Fused filament fabrication (FFF) is one of the most commonly used additive manufacturing technologies. However, the applied material for commercial FFF is limited. Presently, though being one of the most used polymer materials, polypropylene (PP) is rarely used in FFF because of its serious warpage and shrinkage problems. This study investigated the impact of addition of short glass fibers (GF) and ethylene propylene diene monomer (EPDM) on the printability of polypropylene random copolymer (PPR) based FFF and mechanical properties of the printed samples, as well as other properties including rheology, thermal behaviors, and morphology. The results show that the modified PPR has excellent printability, as the printed samples are of good geometrical accuracy. The addition of GF can significantly improve the strength and modulus of the composite, but it also leads to serious decrease in toughness. EPDM addition can effectively improve the toughness of the composite, showing a complementary effect with GF. This work has important meaning in expanding the FFF applicable material and in broadening the application of PP.
Xanthate-Modified Magnetic Fe3O4@SiO2-Based Polyvinyl Alcohol/Chitosan Composite Material for Efficient Removal of Heavy Metal Ions from Water
Authors: Wang Shifan , Liu Yuan , Yang Aiwen , Zhu Qi , Sun Hua , Sun Po , Yao Bing , Zang Yunxiao , Du Xihua , Dong Liming
Published: March 2022

Chitosan has several shortcomings that limit its practical application for the adsorption of heavy metals: mechanical instability, a challenging separation and recovery process, and low equilibrium capacity. This study describes the synthesis of a magnetic xanthate-modified polyvinyl alcohol and chitosan composite (XMPC) for the efficient removal and recovery of heavy metal ions from aqueous solutions. The XMPC was synthesized from polyvinyl alcohol, chitosan, and magnetic Fe3O4@SiO2 nanoparticles. The XMPC was characterized, and its adsorption performance in removing heavy metal ions was studied under different experimental conditions. The adsorption kinetics fit a pseudo-second-order kinetic model well. This showed that the adsorption of heavy metal ions by the XMPC is a chemical adsorption and is affected by intra-particle diffusion. The equilibrium adsorption isotherm was well described by the Langmuir and Freundlich equations. The XMPC reached adsorption equilibrium at 303 K after approximately 120 min, and the removal rate of Cd(II) ions was 307 mg/g. The composite material can be reused many times and is easily magnetically separated from the solution. This makes the XMPC a promising candidate for widespread application in sewage treatment systems for the removal of heavy metals.
Cost Function Analysis Applied to Different Kinetic Release Models of Arrabidaea chica Verlot Extract from Chitosan/Alginate Membranes
Authors: Concha Luis , Resende Pires Ana Luiza , Moraes Angela Maria , Mas-Hernández Elizabeth , Berres Stefan , Hernandez-Montelongo Jacobo
Published: March 2022

This work focuses on the mathematical analysis of the controlled release of a standardized extract of A. chica from chitosan/alginate (C/A) membranes, which can be used for the treatment of skin lesions. Four different types of C/A membranes were tested: a dense membrane (CA), a dense and flexible membrane (CAS), a porous membrane (CAP) and a porous and flexible membrane (CAPS). The Arrabidae chica extract release profiles were obtained experimentally in vitro using PBS at 37 °C and pH 7. Experimental data of release kinetics were analyzed using five classical models from the literature: Zero Order, First Order, Higuchi, Korsmeyer–Peppas and Weibull functions. Results for the Korsmeyer–Peppas model showed that the release of A. chica extract from four membrane formulations was by a diffusion through a partially swollen matrix and through a water filled network mesh; however, the Weibull model suggested that non-porous membranes (CA and CAS) had fractal geometry and that porous membranes (CAP and CAPS) have highly disorganized structures. Nevertheless, by applying an explicit optimization method that employs a cost function to determine the model parameters that best fit to experimental data, the results indicated that the Weibull model showed the best simulation for the release profiles from the four membranes: CA, CAS and CAP presented Fickian diffusion through a polymeric matrix of fractal geometry, and only the CAPS membrane showed a highly disordered matrix. The use of this cost function optimization had the significant advantage of higher fitting sensitivity.
Eco-Friendly Triboelectric Material Based on Natural Rubber and Activated Carbon from Human Hair
Authors: Chomjun Tanapon , Appamato Intuorn , Harnchana Viyada , Amornkitbamrung Vittaya
Published: March 2022

The triboelectric nanogenerator (TENG) has emerged as a novel energy technology that converts mechanical energy from surrounding environments to electricity. The TENG fabricated from environmentally friendly materials would encourage the development of next-generation energy technologies that are green and sustainable. In the present work, a green triboelectric material has been fabricated from natural rubber (NR) filled with activated carbon (AC) derived from human hair. It is found that the TENG fabricated from an NR-AC composite as a tribopositive material and a poly-tetrafluoroethylene (PTFE) sheet as a tribonegative one generates the highest peak-to-peak output voltage of 89.6 V, highest peak-to-peak output current of 6.9 µA, and can deliver the maximum power density of 242 mW/m2. The finding of this work presents a potential solution for the development of a green and sustainable energy source.
An In Situ Experiment to Evaluate the Aging and Degradation Phenomena Induced by Marine Environment Conditions on Commercial Plastic Granules
Authors: De Monte Cristina , Locritani Marina , Merlino Silvia , Ricci Lucia , Pistolesi Agnese , Bronco Simona
Published: March 2022

In this paper, we present two novel experimental setups specifically designed to perform in situ long-term monitoring of the aging behaviour of commercial plastic granules (HDPE, PP, PLA and PBAT). The results of the first six months of a three year monitoring campaign are presented. The two experimental setups consist of: (i) special cages positioned close to the sea floor at a depth of about 10 m, and (ii) a box containing sand exposed to atmospheric agents to simulate the surface of a beach. Starting from March 2020, plastic granules were put into the cages and plunged in seawater and in a sandboxe. Chemical spectroscopic and thermal analyses (GPC, SEM, FTIR-ATR, DSC, TGA) were performed on the granules before and after exposure to natural elements for six months, in order to identify the physical-chemical modifications occurring in marine environmental conditions (both in seawater and in sandy coastal conditions). Changes in colour, surface morphology, chemical composition, thermal properties, molecular weight and polydispersity, showed the different influences of the environmental conditions. Photooxidative reaction pathways were prevalent in the sandbox. Abrasive phenomena acted specially in the sea environment. PLA and PBAT did not show significant degradation after six months, making the possible reduction of marine pollution due to this process negligible.
Review of the Common Deposition Methods of Thin-Film Pentacene, Its Derivatives, and Their Performance
Authors: Yunus Yusniza , Mahadzir Nurul Adlin , Mohamed Ansari Mohamed Nainar , Tg Abd Aziz Tg Hasnan , Mohd Afdzaluddin Atiqah , Anwar Hafeez , Wang Mingqing , Ismail Ahmad Ghadafi
Published: March 2022

Pentacene is a well-known conjugated organic molecule with high mobility and a sensitive photo response. It is widely used in electronic devices, such as in organic thin-film transistors (OTFTs), organic light-emitting diodes (OLEDs), photodetectors, and smart sensors. With the development of flexible and wearable electronics, the deposition of good-quality pentacene films in large-scale organic electronics at the industrial level has drawn more research attention. Several methods are used to deposit pentacene thin films. The thermal evaporation technique is the most frequently used method for depositing thin films, as it has low contamination rates and a well-controlled deposition rate. Solution-processable methods such as spin coating, dip coating, and inkjet printing have also been widely studied because they enable large-scale deposition and low-cost fabrication of devices. This review summarizes the deposition principles and control parameters of each deposition method for pentacene and its derivatives. Each method is discussed in terms of experimentation and theory. Based on film quality and device performance, the review also provides a comparison of each method to provide recommendations for specific device applications.
Influence of the Unit Cell Parameters on the Thermomechanical Non-Symmetric In-Plane Shear Behavior of 2D Biaxial Braided Preform for Thermoplastic Biocomposites
Authors: Zhai Wenqian , Soulat Damien , Legrand Xavier , Wang Peng
Published: March 2022

The identification of thermomechanical in-plane shear behavior of preform is one of the most important factors to ensure the quality of the thermoplastic composites during the thermoforming process. In this present work, the non-symmetric in-plane shear behavior of flax/polypropylene 2D biaxial braided preform for thermoplastic biocomposites was characterized at elevated temperature chamber by using bias-extension test. Analytical models of a bias-extension test based on non-symmetric unit cell geometry for 2D biaxial braids were defined and applied; the thermo-condition-dependent experiments were conducted to study the temperature and displacement rate dependences. The influence of unit cell geometry parameters including braiding angle, tow waviness, and cover factor on the thermal in-plane shear behavior was deeply invested, experiments in both axial and transversal directions were performed for a complete study, and asymmetric scissor mechanisms for in-plane shear behavior were introduced and studied. Finally, a simulation of thermal impregnation distribution based on unit cell geometry was made to clarify the importance of the overall fiber volume fraction.