bluuebunny/crossref_metadata_2025 huggingface dataset:
The natural history of infantile neuroaxonal dystrophy
Authors: Altuame Fadie D. , Foskett Gretchen , Atwal Paldeep S. , Endemann Sarah , Midei Mark , Milner Peter , Salih Mustafa A. , Hamad Muddathir , Al-Muhaizea Mohammad , Hashem Mais , Alkuraya Fowzan S.
Published: May 2020

Background

Infantile neuroaxonal dystrophy (INAD) is a rapidly progressive neurodegenerative disorder of early onset causing premature death. It results from biallelic pathogenic variants in PLA2G6, which encodes a calcium-independent phospholipase A2.

Objective

We aim to outline the natural history of INAD and provide a comprehensive description of its clinical, radiological, laboratory, and molecular findings.

Materials and methods

We comprehensively analyzed the charts of 28 patients: 16 patients from Riyadh, Saudi Arabia, 8 patients from North and South America and 4 patients from Europe with a molecularly confirmed diagnosis of PLA2G6-associated neurodegeneration (PLAN) and a clinical history consistent with INAD.

Results

In our cohort, speech impairment and loss of gross motor milestones were the earliest signs of the disease. As the disease progressed, loss of fine motor milestones and bulbar dysfunction were observed. Temporo-frontal function was among the last of the milestones to be lost. Appendicular spastic hypertonia, axial hypotonia, and hyperreflexia were common neurological findings. Other common clinical findings include nystagmus (60.7%), seizures (42.9%), gastrointestinal disease (42.9%), skeletal deformities (35.7%), and strabismus (28.6%). Cerebellar atrophy and elevations in serum AST and LDH levels were consistent features of INAD. There was a statistically significant difference when comparing patients with non-sense/truncating variants compared with missense/in-frame deletions in the time of initial concern (p = 0.04), initial loss of language (p = 0.001), initial loss of fine motor skills (p = 0.009), and initial loss of bulbar skills (p = 0.007).

Conclusion

INAD is an ultra-rare neurodegenerative disorder that presents in early childhood, with a relentlessly progressive clinical course. Knowledge of the natural history of INAD may serve as a resource for healthcare providers to develop a targeted care plan and may facilitate the design of clinical trials to treat this disease.
Ubiquitination of the DNA-damage checkpoint kinase CHK1 by TRAF4 is required for CHK1 activation
Authors: Yu Xinfang , Li Wei , Liu Haidan , Deng Qipan , Wang Xu , Hu Hui , Xu-Monette Zijun Y. , Xiong Wei , Lu Zhongxin , Young Ken H. , Wang Wei , Li Yong
Published: May 2020

Background

Aberrant activation of DNA damage response (DDR) is a major cause of chemoresistance in colorectal cancer (CRC). CHK1 is upregulated in CRC and contributes to therapeutic resistance. We investigated the upstream signaling pathways governing CHK1 activation in CRC.

Methods

We identified CHK1-binding proteins by mass spectrometry analysis. We analyzed the biologic consequences of knockout or overexpression of TRAF4 using immunoblotting, immunoprecipitation, and immunofluorescence. CHK1 and TRAF4 ubiquitination was studied in vitro and in vivo. We tested the functions of TRAF4 in CHK1 phosphorylation and CRC chemoresistance by measuring cell viability and proliferation, anchorage-dependent and -independent cell growth, and mouse xenograft tumorigenesis. We analyzed human CRC specimens by immunohistochemistry.

Results

TRAF4 catalyzed the ubiquitination of CHK1 in multiple CRC cell lines. Following DNA damage, ubiquitination of CHK1 at K132 by TRAF4 is required for CHK1 phosphorylation and activation mediated by ATR. Notably, TRAF4 was highly expressed in chemotherapy-resistant CRC specimens and positively correlated with phosphorylated CHK1. Furthermore, depletion of TRAF4 impaired CHK1 activity and sensitized CRC cells to fluorouracil and other chemotherapeutic agents in vitro and in vivo.

Conclusions

These data reveal two novel steps required for CHK1 activation in which TRAF4 serves as a critical intermediary and suggest that inhibition of the ATR–TRAF4–CHK1 signaling may overcome CRC chemoresistance.
The application and prospect of CDK4/6 inhibitors in malignant solid tumors
Authors: Du Qi , Guo Xiang , Wang Miao , Li Yongfu , Sun Xiaoyi , Li Qin
Published: May 2020

Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors, which block the transition from the G1 to S phase of the cell cycle by interfering with Rb phosphorylation and E2F release, have shown potent antitumor activity and manageable toxicity in HR+/HER2− breast cancer patients. Some clinical trials involving CDK4/6 inhibitors in other tumors have achieved preliminary impressive efficacy. Whether CDK4/6 inhibitors possess great potential as broad-spectrum antitumor drugs and how to maximize their clinical benefits remain uncertain. TCGA database analysis showed that CDK4/6 genes and related genes are widely expressed among various tumors, and high or moderate expression of CDK4/6 genes commonly indicates poor survival. CDK4/6 gene expression is significantly higher in COAD, ESCA, STAD, LIHC, and HNSC, suggesting that CDK4/6 inhibitors could be more efficacious in those tumors. Moreover, network analysis with the STRING database demonstrated that CDK4/6-related proteins were co-expressed or co-occurred with the classical tumor signaling pathways, such as the cell cycle pathway, RAS pathway, PI3K pathway, Myc pathway, and p53 pathway. The extensive antitumor effects of CDK4/6 inhibitors may be achieved by synergizing or antagonizing with other signaling molecule inhibitors, and combination therapy might be the most effective treatment strategy. This article analyzed the feasibility of expanding the application of CDK4/6 inhibitors at the genetic level and further summarized the associated clinical/preclinical studies to collect supportive evidence. This is the first study that presents a theoretical foundation for CDK4/6 inhibitor precision therapy via combined analysis of comprehensive gene information and clinical research results.
Peer support for people with severe mental illness versus usual care in high-, middle- and low-income countries: study protocol for a pragmatic, multicentre, randomised controlled trial (UPSIDES-RCT)
Authors: Moran Galia S. , Kalha Jasmine , Mueller-Stierlin Annabel S. , Kilian Reinhold , Krumm Silvia , Slade Mike , Charles Ashleigh , Mahlke Candelaria , Nixdorf Rebecca , Basangwa David , Nakku Juliet , Mpango Richard , Ryan Grace , Shamba Donat , Ramesh Mary , Ngakongwa Fileuka , Grayzman Alina , Pathare Soumitra , Mayer Benjamin , Puschner Bernd
Published: May 2020

Background

Peer support is an established intervention involving a person recovering from mental illness supporting others with mental illness. Peer support is an under-used resource in global mental health. Building upon comprehensive formative research, this study will rigorously evaluate the impact of peer support at multiple levels, including service user outcomes (psychosocial and clinical), peer support worker outcomes (work role and empowerment), service outcomes (cost-effectiveness and return on investment), and implementation outcomes (adoption, sustainability and organisational change).

Methods

UPSIDES-RCT is a pragmatic, parallel-group, multicentre, randomised controlled trial assessing the effectiveness of using peer support in developing empowering mental health services (UPSIDES) at four measurement points over 1 year (baseline, 4-, 8- and 12-month follow-up), with embedded process evaluation and cost-effectiveness analysis. Research will take place in a range of high-, middle- and low-income countries (Germany, UK, Israel, India, Uganda and Tanzania). The primary outcome is social inclusion of service users with severe mental illness (N = 558;N = 93 per site) at 8-month follow-up, measured with the Social Inclusion Scale. Secondary outcomes include empowerment (using the Empowerment Scale), hope (using the HOPE scale), recovery (using Stages of Recovery) and health and social functioning (using the Health of the Nations Outcome Scales). Mixed-methods process evaluation will investigate mediators and moderators of effect and the implementation experiences of four UPSIDES stakeholder groups (service users, peer support workers, mental health workers and policy makers). A cost-effectiveness analysis examining cost-utility and health budget impact will estimate the value for money of UPSIDES peer support.

Discussion

The UPSIDES-RCT will explore the essential components necessary to create a peer support model in mental health care, while providing the evidence required to sustain and eventually scale-up the intervention in different cultural, organisational and resource settings. By actively involving and empowering service users, UPSIDES will move mental health systems toward a recovery orientation, emphasising user-centredness, community participation and the realisation of mental health as a human right.

Trial registration

ISRCTN,ISRCTN26008944. Registered on 30 October 2019.
Validity and diagnostic performance of fluorescence optical imaging measuring synovitis in hand osteoarthritis: baseline results from the Nor-Hand cohort
Authors: Maugesten Øystein , Mathiessen Alexander , Hammer Hilde Berner , Hestetun Sigrid Valen , Kvien Tore Kristian , Uhlig Till , Ohrndorf Sarah , Haugen Ida Kristin
Published: May 2020

Objective

Fluorescence optical imaging (FOI) demonstrates enhanced microcirculation in finger joints as a sign of inflammation. We wanted to assess the validity and diagnostic performance of FOI measuring synovitis in persons with hand OA, comparing it with magnetic resonance imaging (MRI)- and ultrasound-detected synovitis.

Methods

Two hundred and twenty-one participants with hand OA underwent FOI and ultrasound (gray-scale synovitis and power Doppler activity) of the bilateral hands and contrast-enhanced MRI examination of the dominant hand. Fifteen joints in each hand were scored on semi-quantitative scales (grade 0–3) for all modalities. Four FOI images were evaluated: one composite image (Prima Vista Mode (PVM)) and three images representing phases of fluorescent dye distribution. Spearman’s correlation coefficients were calculated between sum scores of FOI, MRI, and ultrasound. Sensitivity, specificity, and area under the curve (AUC) were calculated for FOI using MRI or ultrasound as reference.

Results

FOI did not demonstrate enhancement in the thumb base, and the joint was excluded from further analyses. FOI sum scores showed poor to fair correlations with MRI (rho 0.01–0.24) and GS synovitis sum scores (rho 0.12–0.25). None of the FOI images demonstrated both good sensitivity and specificity, and the AUC ranged from 0.50–0.61 and 0.51–0.63 with MRI and GS synovitis as reference, respectively. FOI demonstrated similar diagnostic performance with PD activity and GS synovitis as reference.

Conclusion

FOI enhancement correlated poorly with synovitis assessed by more established imaging modalities, questioning the value of FOI for the evaluation of synovitis in hand OA.
MiR-21-5p regulates extracellular matrix degradation and angiogenesis in TMJOA by targeting Spry1
Authors: Ma Shixing , Zhang Aobo , Li Xiaole , Zhang Shizhou , Liu Shaopeng , Zhao Haoming , Wu Shichao , Chen Lei , Ma Chuan , Zhao Huaqiang
Published: May 2020

Background

Due to the lack of research on the pathological mechanism of temporomandibular joint osteoarthritis (TMJOA), there are few effective treatment measures in the clinic. In recent years, microRNAs (miRs) have been demonstrated to play an important role in the pathogenesis of osteoarthritis (OA) by regulating a variety of target genes, and the latest evidence shows that miR-21-5p is specifically overexpressed in OA. The purpose of this project was to clarify whether miR-21-5p can regulate the TMJOA process by targeting Spry1.

Methods

TMJOA was induced by a unilateral anterior crossbite (UAC) model, and the effect of miR-21-5p knockout on TMJOA was evaluated by toluidine blue (TB), immunohistochemical (IHC) staining, Western blotting (WB) and RT-qPCR. Primary mouse condylar chondrocytes (MCCs) were isolated, cultured and transfected with a series of mimics, inhibitors, siRNA-Spry1 or cDNA Spry1. WB, RT-qPCR, IHC and TB were used to detect the effect of miR-21-5p and its target gene Spry1 on the expression of MMP-13, VEGF and p-ERK1/2 in TMJOA. The effect of miR-21-5p on angiogenesis was evaluated by chick embryo chorioallantoic membrane (CAM) assay and WB.

Results

In the UAC model, the cartilage thickness and extracellular matrix of miR-21-5p knockout mice were less damaged, and miR-21-5p and UAC model were shown to affect the expression of Spry1, IL-1β, MMP-13, and VEGF. Luciferase experiments confirmed that Spry1 was the direct target of miR-21-5p. The expression levels of Spry1, MMP-13, VEGF and p-ERK1/2 in MCCs transfected with miR-21-5p mimic were higher than those in the inhibitor group. Under the simulated inflammatory environment of IL-1β, the expression levels of MMP-13, VEGF and p-ERK1/2 were positively correlated with miR-21-5p, while Spry1 was negatively correlated with miR-21-5p. Inhibition of miR-21-5p expression and overexpression of Spry1 enhanced the inhibition of MMP-13, VEGF and p-ERK1/2 expression. MiR-21-5p had a significant role in promoting angiogenesis in the chick embryo CAM assay, and this role was clearly mediated by the ERK-MAPK signalling pathway.

Conclusion

This study verified that miR-21-5p can promote the process of TMJOA by targeting Spry1, which provides a new direction for future research on the treatment of this disease.
Is real time PCR preferable to the direct immunofluorescence in the diagnosis of Pneumocystis jirovecii pneumonia in HIV-infected patients?
Authors: Bossart Simon , Mühlethaler Konrad , Garzoni Christian , Furrer Hansjakob
Published: May 2020

Objectives

In this study, we compared IFA and real-time PCR in bronchoalveolar lavage specimens of HIV infected patients. A total of 66 BALs from 62 HIV patients were included in the study. 30 IFA positive and 36 IFA negative specimens were tested with real-time PCR, targeting the major surface glycoprotein. We performed a retrospective analysis of the patient’s medical records, compared the results of the IFA and PCR tests and analyzed costs, expenditure of time and personal expenses.

Results

All of the 30 IFA positive samples were PCR positive. 35 of 36 IFA negative probes were also negative in the PCR assay. Considering the PCR results as a binary outcome (positive/negative) sensitivity was 100%, specificity 97.2%. The patient with negative IFA and positive PCR had a clear clinical picture of PCP and responded to PCP treatment. PCR was more than twice as expensive and time-consuming as IFA. Diagnostic accuracy for PCP of PCR and IFA was comparable in HIV-infected patients, but IFA was significantly less expensive and less time-consuming. Therefore, IFA testing can continue to be used as gold standard in the diagnosis of PCP in HIV patients. However, in special cases, IFA may lack sensitivity and PCR should be added to the diagnostic armamentarium.
Sex-related differences in markers of immune activation in virologically suppressed HIV-infected patients
Authors: Santinelli Letizia , Ceccarelli Giancarlo , Borrazzo Cristian , Innocenti Giuseppe Pietro , Frasca Federica , Cavallari Eugenio Nelson , Celani Luigi , Nonne Chiara , Mastroianni Claudio Maria , d’Ettorre Gabriella
Published: May 2020

Objectives

Gender-specific studies remain a neglected area of biomedical research. Recent reports have emphasized that sex-related biological factors may affect disease progression during HIV-1 infection. The aim of this study was to investigate the influence of sex on the levels of immune activation in the gut and in peripheral blood of individuals with HIV treated with fully suppressive antiretroviral therapy (ART).

Methods

Thirty individuals with HIV undergoing long-term fully suppressive ART were enrolled in this study. Lamina propria lymphocytes (LPL) and peripheral blood mononuclear cells (PBMCs) were isolated from gut biopsies collected by pancolonoscopy and peripheral blood samples. The expression of markers of immune activation was evaluated by multi-parametric flow cytometry. This is a sub analysis of ClinicalTrials.gov Identifier: NCT02276326

Results

We observed differences in the levels of immune activation in the gut and in PBMCs, with values higher in the gut compartment compared to PBMCs.

In addition, we found that the mean value of the levels of immune activation was higher in the women than in the men. Finally, we measured the markers of immune activation by mean relative difference (MRD) and confirmed the higher value in the women.

Conclusion

A significant sex-related difference in the level of immune activation was observed in a population of individuals with HIV on long-term ART. A more complete characterization of these differences may support the introduction of sex-specific approaches in the clinical management of individuals with HIV.
Elderly bedridden patients with dementia use over one quarter of resources in internal medicine wards in an Israeli hospital
Authors: Salz Inbal Weiss , Carmeli Yehuda , Levin Avi , Fallach Noga , Braun Tali , Amit Sharon
Published: May 2020

Background

Elderly bedridden patients with dementia (EBRPD) are a growing segment of the population. We aimed to describe acute care hospitalization of EBRPD in internal medicine wards: the prevalence of EBRPD, their impact on hospital resources and hospital ecology, one-year survival, and one-year readmission-free survival.

Methods

The study setting was the internal medicine division of one tertiary care hospital in Israel. We conducted a point-prevalence survey to measure the prevalence of EBRPD and the prevalence of multidrug-resistant organism (MDRO) carriage. We also conducted a retrospective chart review of EBRPD who were hospitalized in the internal medicine division in order to assess resource use, survival, and readmission.

Results

In the point prevalence surveys (N = 1667 patients), EBRPD comprised 24.3% of patients and 59.0% of mechanically ventilated patients. EBRPD were twice as likely to be colonized or infected by MDROs as other patients (39.3% vs. 18%, p < 0.001); thus, 41% of MDRO carriers during the survey days were EBRPD. In the retrospective study (N = 517 EBRPD), 80% of EBRPD received antibiotics; on average, they received an antibiotic on 87.7% of their hospital days. One-year survival was 35.6% and one-year readmission-free survival was 16.4%.

Conclusions

Acute care hospitalization of EBRPD accounted for a high proportion of bed occupancy and ventilator use in internal medicine wards. EBRPD significantly increase the potential for MDRO transmission. Policymakers should seek alternatives to acute care hospitalization for EBRPD.
Iodine deficiency in Israeli pregnant women – a time for action
Authors: Lazarus John H.
Published: May 2020

Iodine is an essential micronutrient for thyroid gland function. Iodine deficiency disorders are a spectrum of conditions affecting the fetus, child, adolescent and adult. Iodine requirements are increased in pregnancy in order that the fetus receives enough maternal thyroxine via transplacental passage. Previous studies in Israel have shown widespread iodine deficiency by measurement of urinary iodine concentrations in school age children and adults. The present study clearly shows iodine deficiency in a group of 2nd trimester women as judged by measurement of serum thyroglobulin (a recently evaluated marker of iodine deficiency). An additional factor in this work is that the subjects all resided in an area using desalinated water. Desalination has previously been shown to significantly reduce the iodine content of water compared to water in Israel not subject to this process.

The data in this group of pregnant women should serve as a wakeup call to the public health community to correct this deficiency which is known to significantly affect child neurodevelopment. There are several issues to be addressed. The knowledge base relating to iodine nutrition especially during pregnancy is low. An educational plan is required. The strategy for achieving adequate iodine nutrition in the population and particularly before and during pregnancy requires urgent review. While iodine supplementation before and during gestation can correct iodine deficiency, the provision of iodised salt in the community is recommended, similar to more than 80% of countries in the world.

It is indeed a time for action to ensure the adequate intellectual performance of Israel’s children.
Time-frequency feature extraction for classification of episodic memory
Authors: Anderson Rachele , Sandsten Maria
Published: May 2020

This paper investigates the extraction of time-frequency (TF) features for classification of electroencephalography (EEG) signals and episodic memory. We propose a model based on the definition of locally stationary processes (LSPs), estimate the model parameters, and derive a mean square error (MSE) optimal Wigner-Ville spectrum (WVS) estimator for the signals. The estimator is compared with state-of-the-art TF representations: the spectrogram, the Welch method, the classically estimated WVS, and the Morlet wavelet scalogram. First, we evaluate the MSE of each spectrum estimate with respect to the true WVS for simulated data, where it is shown that the LSP-inference MSE optimal estimator clearly outperforms other methods. Then, we use the different TF representations to extract the features which feed a neural network classifier and compare the classification accuracies for simulated datasets. Finally, we provide an example of real data application on EEG signals measured during a visual memory encoding task, where the classification accuracy is evaluated as in the simulation study. The results show consistent improvement in classification accuracy by using the features extracted from the proposed LSP-inference MSE optimal estimator, compared to the use of state-of-the-art methods, both for simulated datasets and for the real data example.
Approximation by modified Kantorovich–Szász type operators involving Charlier polynomials
Authors: Ansari K. J. , Mursaleen M. , Shareef KP M. , Ghouse M.
Published: May 2020

In this paper, we give some direct approximation results by modified Kantorovich–Szász type operators involving Charlier polynomials. Further, approximation results are also developed in polynomial weighted spaces. Moreover, for the functions of bounded variation, approximation results are proved. Finally, some graphical examples are provided to show comparisons of convergence between old and modified operators towards a function under different parameters and conditions.
Effect of dietary supplementation with yeast cell wall extracts on performance and gut response in broiler chickens
Authors: Pascual A. , Pauletto M. , Giantin M. , Radaelli G. , Ballarin C. , Birolo M. , Zomeño C. , Dacasto M. , Bortoletti M. , Vascellari M. , Xiccato G. , Trocino A.
Published: May 2020

Background

The dietary supplementation of yeast cell wall extracts (YCW) has been found to reduce pathogenic bacteria load, promote immunoglobulin production, prevent diseases by pro-inflammatory responses, and alter gut microbiota composition. This study evaluated growth and slaughter results, health, gut morphology, immune status and gut transcriptome of 576 male chickens fed two diets, i.e. C (control) or Y (with 250–500 g/t of YCW fractions according to the growth period). At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation, with Alcian-PAS for goblet cells, and antibodies against CD3+ intraepithelial T-cells and CD45+ intraepithelial leukocytes. The jejunum sampled at 42 d were also used for whole-transcriptome profiling.

Results

Dietary YCW supplementation did not affect final live weight, whereas it decreased feed intake (114 to 111 g/d; P ≤ 0.10) and improved feed conversion (1.74 to 1.70; P ≤ 0.01). Regarding the gut, YCW supplementation tended to increase villi height (P = 0.07); it also increased the number of goblet cells and reduced the density of CD45+ cells compared to diet C (P < 0.001). In the gut transcriptome, four genes were expressed more in broilers fed diet Y compared to diet C, i.e. cytochrome P450, family 2, subfamily C, polypeptide 23b (CYP2C23B), tetratricopeptide repeat domain 9 (TTC9), basic helix-loop-helix family member e41 (BHLHE41), and the metalloreductase STEAP4. Only one gene set (HES_PATHWAY) was significantly enriched among the transcripts more expressed in broilers fed diet Y. However, a total of 41 gene sets were significantly over-represented among genes up-regulated in control broilers. Notably, several enriched gene sets are implicated in immune functions and related to NF-κB signaling, apoptosis, and interferon signals.

Conclusions

The dietary YCW supplementation improved broiler growth performance, increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut transcriptome, which can be considered useful to improve animal welfare and health under the challenging conditions of intensive rearing systems in broiler chickens.
Perceived social support and psychological wellbeing among Nepalese adolescents: the mediating role of self-esteem
Authors: Poudel Anju , Gurung Bishnu , Khanal Gopal Prasad
Published: May 2020

Background

Adolescence is characterized by unique, multiple physical, psychological and social development. Understanding the well-being of adolescents and the factors that contribute to it will help towards clarifying and defining ways to better help adolescents prepare for adult life. Therefore, the present study aims to find out the relationship between Perceived Social Support (PSS) and Psychological Well-Being (PWB) among Nepalese adolescence based on mediating role of Self-esteem (SE).

Methods

The study was conducted among 348 adolescents studying in grade 9 and 10 of government secondary level schools of Pokhara Metropolitan city, Nepal. Data were collected through self-administered standard tools-Multidimensional Scale of Perceived Social Support (MSPSS), Rosenberg self-esteem scale (RSES) and General Health Questionnaire (GHQ-12). For statistical analysis descriptive statistics, correlation, regression and mediation analyses were used. The statistical significance of mediating effect of the tested model was examined through a path proposed by Baron and Kenny and Bootstrap method.

Results

Overall, the finding suggests that PSS indirectly affects PWB of adolescents through mediating variable SE. Adolescents who perceive good social support had higher SE, which in turn contributed to their PWB. Furthermore, the study found no significant gender difference for PSS, SE and PWB. Also among various sources of PSS, both boys and girls were more oriented towards family for social support than friends and others.

Conclusion

Adolescents who experience higher social support are likely to have higher SE and are more likely to have better PWB. The findings of the study will be useful to the parents, teachers, counselors, psychologist and researchers to develop strategies to enhance adolescent’s mental health.
Deep groundwater discharge after the 2011 Mw 6.6 Iwaki earthquake, Japan
Authors: Sato Tsutomu , Kazahaya Kohei , Matsumoto Norio , Takahashi Masaaki
Published: May 2020

Hot spring discharge was linked to the 2011 Mw 6.6 Iwaki earthquake. Periodic surveys revealed that the discharge continued for more than 7 years, which is a rare and valuable long-term record of hot spring discharge triggered by an earthquake in a non-volcanic area. In terms of coseismic changes, based on a comparison of the spatial distribution of changes in the coseismic water head and calculated crustal volumetric strain using a fault model, hot spring water discharge was found to be caused by a change in the coseismic crustal volumetric strain. As for the postseismic changes, observations over 7 years revealed a gradual rise in the temperature and chloride ion concentration of the hot spring water. Such long-term hot spring discharge may be explained by the following two causes: the rise of thermal water from the deep part and the permeability changes along the hot spring channels.
New hypothesis to explain Quaternary forearc deformation and the variety of plate boundary earthquakes along the Suruga–Nankai Trough by oblique subduction of undulations on the Philippine Sea Plate
Authors: Okamura Yukinobu , Shishikura Masanobu
Published: May 2020

Plate-boundary earthquakes of magnitude 8 or greater along the Suruga–Nankai Trough subduction zone have repeated at intervals of 90–150 years, but with widely varying magnitudes and rupture areas. We propose, based on geologic data on crustal movements of the forearc wedge, that these earthquake variations are controlled by two separate locked zones in the deeper part of the plate boundary. Long-wavelength topographic undulations, composed of alternating zones of uplift and subsidence along the forearc wedge, are associated with 2000 to 3000 m of vertical relief that has accumulated during Quaternary time. We suggest that this crustal deformation in the forearc wedge is caused not by stress loading and release during earthquake cycles, but rather by vertical displacements of the plate boundary caused by the westward movement of undulations in the obliquely subducting slab of the Philippine Sea Plate as it subducts beneath Southwest Japan. Dating of emergent marine shell fossil assemblages shows that the Kii Mountains, an uplift zone at the midpoint of the trough, has undergone uplift events at intervals of 400–600 year, and the latest event of those occurred when the 1707 Hoei earthquake ruptured the entire plate boundary along the trough. We infer that the plate boundary under the Kii Mountains is a locked zone and that slips of this zone, which accompanied ruptures of the entire plate boundary, caused uplift of the mountains by decreasing the plate boundary depth. A similar locked zone is inferred under the uplift zone of the Akaishi Mountains, along the eastern margin of the trough, and the 1854 Ansei earthquake pair was presumably caused by the slip of this zone. During the two 1854 events, the locked zone under the Kii Mountains presumably restricted the rupture propagation to the eastern half of the trough, then a rupture of the western half of the trough followed within 32 h. These locked zones are inferred to slip independently every few hundred years and determine the major patterns of characteristic ruptures along the Suruga–Nankai Trough.
Abortion attitudes, training, and experience among medical students in Jamaica, West Indies
Authors: Matthews Glenmarie , Atrio Jessica , Fletcher Horace , Medley Nathalie , Walker Leo , Benfield Nerys
Published: May 2020

Objective

To define abortion attitudes, training and experience among medical students in Jamaica, a restricted environment for legal abortion.

Method

From September to November 2017 we conducted an anonymous online cross-sectional survey among medical students enrolled at the University of West Indies (UWI) in Jamaica. An abortion attitudes sum score was used for analysis. Multivariate regression was applied to evaluate the impact of characteristics and experiences on abortion attitudes.

Results

The primary outcome was a validated composite abortion attitudes sum score, ranging from zero to forty-five. 1404 students completed the survey for a response rate of 88%. 64% had a positive attitude towards abortion. In multivariate analysis, medical students’ attitudes were favorably impacted by a prior personal or family experience with abortion, identifying as non-religious, being older in age and mixed raced. 1321 (94%) agreed that abortion training should be included in the medical school curriculum. 78.8% reported no abortion training and only 17.9% reported miscarriage management training.

Conclusion

Medical students at UWI had favorable attitudes towards abortion, despite their limited training in a restrictive environment. Prior personal experience with abortion and being non-religious were the strongest predictor of favorable attitudes. Increased training and clinical exposure may prove to be crucial in improving access of safe abortion.
Osteochondrogenesis derived from synovial fibroblasts in inflammatory arthritis model
Authors: Miura Yoko , Kanazawa Satoshi
Published: May 2020

Rheumatoid arthritis (RA) is characterized by chronic joint inflammation, which forms pannus with bone destruction. Bony ankylosis is also observed following inflammation; however, the mechanism behind this aberrant bone formation in RA had remained unclear. Based on our recent findings obtained using a novel arthritis model called D1BC mouse, we found that synovial fibroblasts in pannus consist of at least three different populations with the osteochondrogenic lineage being predominant. We also found endochondral ossification like that in embryonic bone development adjacent to invasive synovial fibroblasts. Such ectopic endochondral ossification leads to the failure of bone repair and results in ankylosis. In this review, we describe the character of synovial fibroblasts toward the osteochondrogenic lineage and ectopic endochondral ossification in an inflammatory arthritis mouse model.
Pathogenicity of three entomopathogenic fungi against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
Authors: Soliman N. A. , Al-amin Sherihan M. , Mesbah Amira E. , Ibrahim Ahmed M. A. , Mahmoud Ali M.A.
Published: May 2020

The use of pesticides against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) as a tool to control the pest has become an obstacle to the fresh agricultural products export to many countries that restrict pesticides residues. The effectiveness of three local strains of entomopathogenic fungi: Metarhizium anisopliae, Beauveria bassiana, and Paecilomyces lilacinus against the adult and immature stages of C. capitata was evaluated under laboratory conditions. Obtained results showed that M. anisopliae and B. bassiana were superior in its pathogenicity and potential to kill the pest than P. lilacinus. These results may be important to be used for the control of the pest in IPM program.
Recent Research on Students’ Identities: Advancing Theory and Practice to Disrupt Inequities
Authors: Gouvea Julia
Published: May 2020

This installment of Current Insights features recent research on identity development, with implications for equity in science and mathematics education.
Annual Meeting Update
Authors: Menyoli Elive , Abriel Bill
Published: May 2020

In addition to the substantial technical program provided during the SEG Annual Meeting, there are also special sessions designed to bring focus to important and relevant topics of interest to attendees. A global technical session this year will highlight Africa while the Business of Applied Geophysics (BAGS) plenary sessions will highlight several additional topics of interest. We believe these special sessions fit the needs and interests of many, and we look forward to seeing you 11–16 October in Houston.
Introduction to this special section: Near-surface imaging and modeling
Authors: Sloan Steve , Feigenbaum Dan
Published: May 2020

This special section on near-surface imaging and modeling was intended originally to focus on improving deeper imaging for exploration purposes through more accurate representations of the near surface, the highly variable zone that energy must traverse through on the way down and back up again to be recorded at the surface. However, as proposed manuscript topics started coming in, it became clear that this section would cover a wider range, from kilometers down to meters. Papers in this section highlight a range of near-surface-related work that includes applying full-waveform inversion (FWI) to improve near-surface velocity models, identifying potential sinkhole hazards before they collapse, the potential of smartphones as geophysical sensors, and new open-source software for ground-penetrating radar data.
Time-lapse monitoring of stress-field variations within the Lower Permian shales in Kansas
Authors: Morton Sarah L. , Miller Richard D. , Ivanov Julian , Peterie Shelby L. , Parsons Robert L.
Published: May 2020

Azimuthal stress-field variations in Permian shale overlying voids in salt appear related to localized void roof failure based on interpretations of time-lapse recordings of passive seismic surface-wave data. Salt-dissolution voids in south-central Kansas exist within the Hutchinson Salt Member as a result of solution mining operations early in the 20th century. Since shear-wave velocity can be estimated from surface-wave analysis and is directly related to the shear modulus, or material rigidity, it can be used to indicate shale cap-rock competency. Temporal bulk-velocity variations in bedrock acquired over a known void from 2013 to 2018 are consistent with cyclic stress-field behavior expected with repetitive localized failure within the shale cap rock. It was determined that roof failure and associated subsidence began prior to the 2013 surveys along a north–south failure plane with consistent progression from southwest to northeast until 2017. A low-velocity anomaly observed in 2014–2015 on both profiles indicated a load redistribution in the shale cap rock. This low-velocity region was gradually replaced by an increasing velocity field in subsequent years. These bulk-velocity variations are interpreted as representations of stoping, which occurs when overlying roof layers or sidewall segments progressively fail. As of 2018, the velocity trend in the affected area remained lower than the recorded baseline conditions, with no low-velocity anomalies observed since 2015. Shear-wave velocities are not likely to return to 2013 conditions due to loss of roof material and redistribution of load. Annual surveys will monitor for conditions consistent with the beginning of a stress buildup and roof collapse unless monitoring indicates a change in the subsurface that warrants invasive analysis or other actions.
GPRPy: Open-source ground-penetrating radar processing and visualization software
Authors: Plattner Alain M.
Published: May 2020

GPRPy is an open-source ground-penetrating radar software compatible with a range of ground-penetrating radar systems. Data processing and plotting can be performed by using graphical user interfaces or scripts that are generated automatically from the graphical user interfaces. This makes learning the software easy, and it enables researchers to share their scripts as part of a publication to ensure reproducible research. GPRPy enables profile data processing and visualization, velocity analysis, interpolation of 3D data cubes from profile data, and 3D interpolation for interfaces visible in multiple profiles. The software is written in Python and runs on all major operating systems.
Compressive seismic data acquisition in a desert area of western China: A case study
Authors: Shu Guoxu , Shi Taikun , Huang Liang , Gao Zhenghui , Lv Gonghe , Di Zhixin , Huo Shoudong
Published: May 2020

The seismic exploration industry continuously demands better imaging quality and consequently requires denser spatial sampling, which increases acquisition cost and time. To alleviate this burden, compressive sensing (CS) theory has been introduced in the design of acquisition geometry, as it requires fewer shot and receiver locations than traditional methods. In 2017, we conducted a field experiment in a desert area in western China. This was the first such test in China to utilize CS theory in a field test. The survey had 1760 shot records with irregular shot and receiver locations designed with guidance from CS theory. By way of data reconstruction, a seismic data set with higher sampling density (7.5 × 7.5 m bin size) was acquired, and the imaging quality was improved significantly compared to existing legacy data (15 × 15 m bin size). These results indicate that a CS-designed acquisition may reduce cost while enhancing imaging quality.
Impact of phase rotation on reservoir characterization and implementation of seismic well tie technique for calibration offshore Nile Delta, Egypt
Authors: El-Behiry Mohamed G. , Al Araby Mohamed S. , Ragab Ramy Z.
Published: May 2020

Seismic wavelets are dynamic components that result in a seismic trace when convolved with reflectivity series. The seismic wavelet is described by three components: amplitude, frequency, and phase. Amplitude and frequency are considered static because they mainly affect the appearance of a seismic event. Phase can have a large effect on seismic appearance by changing the way it describes the subsurface. Knowing the wavelet properties of certain seismic data facilitates the process of interpretation by providing an understanding of the appearance of regional geologic markers and hydrocarbon-bearing formation behavior. The process through which seismic data wavelets are understood is called seismic well tie. Seismic well tie is the first step in calibrating seismic data in terms of polarity and phase. It ensures that the seismic data are descriptive to regional markers, well markers, and discoveries (if they exist). The step connects well data to seismic data to ensure that the seismic correctly describes well results at the well location. It then extends the understanding of seismic behavior to the rest of the area covered by the seismic data. Good seismic well tie will greatly reduce uncertainties accompanying seismic interpretation. One important outcome of the seismic well tie process is understanding the phase of seismic data, which affects how seismic data will reflect a known geologic marker or hydrocarbon-bearing zone. This understanding can be useful in quantifying discoveries attached to seismic anomalies and extending knowledge from the well location to the rest of the area covered by seismic data.
Near-surface modeling challenges highlighted in recent workshop
Authors: Saad Abdulaziz , Al-Jahdhami Moosa
Published: May 2020

Despite technological and computational advances in geophysical imaging, near-surface geophysics continues to pose significant challenges in modeling and imaging the subsurface. Geoscientists from around the world attended the first and second editions of the SEG/DGS Near-surface Modeling and Imaging Workshop in 2014 and 2016 to address these challenges. A range of near-surface disciplines were represented from academia and industry, covering aspects of engineering and hydrocarbon exploration. The previous workshops explored emerging and underdeveloped techniques, including deep learning (machine learning), nonseismic methods, full-waveform inversion (FWI), and joint inversion. The necessity to further understand guided waves, anisotropy, velocity inversion, and the creation of an inclusive near-surface model was identified. The previous editions led to a greater understanding of the importance of knowledge sharing among various disciplines in modeling and imaging of the near surface.
Student Zone
Authors: Petrokushyn Roman
Published: May 2020

This is my fourth year as a member of the Kyiv SEG Student Chapter. During my membership, I have taken part in seven field camps, two of which were organized by our student chapter. I can say with confidence that the field camps changed my life and made me who I am today.
Neuronal and glial regulation of CNS angiogenesis and barriergenesis
Authors: Biswas Saptarshi , Cottarelli Azzurra , Agalliu Dritan
Published: May 2020

Neurovascular pathologies of the central nervous system (CNS), which are associated with barrier dysfunction, are leading causes of death and disability. The roles that neuronal and glial progenitors and mature cells play in CNS angiogenesis and neurovascular barrier maturation have been elucidated in recent years. Yet how neuronal activity influences these processes remains largely unexplored. Here, we discuss our current understanding of how neuronal and glial development affects CNS angiogenesis and barriergenesis, and outline future directions to elucidate how neuronal activity might influence these processes. An understanding of these mechanisms is crucial for developing new interventions to treat neurovascular pathologies.
Direct Transformation of Silica from Natural Resources to Form Tetramethoxysilane
Authors: Putro Wahyu S , Fukaya Keisuke , Choi Jun-Chul , Choi Seong Jib , Horikoshi Toshio , Sato Kazuhiko , Fukaya Norihisa
Published: May 2020

A simple and practical method for direct synthesis of tetramethoxysilane (TMOS) from silica (SiO2) and methanol was achieved using a base catalyst and acetal as a dehydrant under carbon dioxide (CO2). The production of TMOS was strongly influenced by the kind of the acetal used, with 2,2-dimethoxypropane identified as the most effective dehydrant. We observed that the acetal used enabled the production of a high yield of dimethyl carbonate (DMC), which promoted the TMOS production. DMC is an intermediate product from the reaction of CO2 and methanol, which supported the SiO2 depolymerization process. When the reaction is conducted with 2,2-dimethoxypropane at 260 °C for 24 h, TMOS can be produced in up to 59% yield. For practical applications, the TMOS synthesis has been developed on a 250 mL and 1 L-scale reaction with constant yield (>50%) from various silica resources.
Mathematics Teachers Reflection on the Role of Productive Pedagogies in Improving their Classroom Instruction
Authors: Bature Iliya Joseph , Atweh Bill
Published: May 2020

This paper sought to investigate the mathematics teachers’ reflection on the role of Productive Pedagogies’ framework in festering effective classroom instruction. Two research objectives were used to discuss the reflection of the four mathematic teachers. A qualitative case study was adopted as the design of the study. Classroom observations, Research Journal and Reflective Interviews were organised to collect data for the study, while the narratives approach to data analysis was used to analyse the data collected. From the study, the mathematics teachers were able to gradually adjust to the new framework through effective reflective meetings with their colleagues and with the support of the researcher. They (mathematics teachers) reflected that, the framework helps them developed confidence, positive attitude and interest to their teaching practice. It helped fostered effective collaboration and created an atmosphere of trust between the mathematics teachers and their students. The study also suggested that the pedagogy influence the mathematics teachers to provide and received feedback from their colleagues and from their students. They were also of the view that there was a gradual shift from the traditional teacher centred instruction they are more conversant with to a more student-centred pedagogy. The implication of the study suggests the need to adopt the Productive Pedagogies framework into the Nigerian mathematics classroom to improve mathematics teacher’s classroom instruction.
A Review on Current Mechanical and Electronic Design Aspects and Future Prospects of Smart Canes for Individuals with Lower Limb Difficulties
Authors: Arefin Paroma , Habib Md Shehan , Arefin Aishawarya , Arefin Md Saidul
Published: May 2020

Smart canes are one of the mobility assistive devices to facilitate the freedom of movement and help people with mobility problems to move around and perform daily chores, which are not possible usually. But they are available in different design options to offer specific advantages. In this review paper, we have addressed different mechanical and electronic designs of assistive devices proposed and developed by various researchers. The aim of our study was to sort out different mechanisms of actions used by them. With the discussion and comparison of their mode of functions, we have found a direction to potential future improvements, development, and variations to fulfill individualized and customized requirements.