bluuebunny/crossref_metadata_2025 huggingface dataset:
Production of Abzymes in Th, CBA, and C57BL/6 Mice before and after MOG Treatment: Comparing Changes in Cell Differentiation and Proliferation
Authors: Aulova Kseniya S. , Urusov Andrey E. , Toporkova Ludmila B. , Sedykh Sergey E. , Shevchenko Yuliya A. , Tereshchenko Valery P. , Sennikov Sergei V. , Budde Thomas , Meuth Sven G. , Popova Nelly A. , Orlovskaya Irina A. , Nevinsky Georgy A.
Published: December 2019

Till yet there is no data concerning mechanisms of autoimmune diseases development. Experimental autoimmune encephalomyelitis (EAE) prone C57BL/6 (T- and B-lymphocyte response), non-autoimmune CBA, and Th mice with T cell response were immunized with myelin oligodendrocyte glycoprotein (MOG35–55) to compare different characteristics of autoimmune reaction development. Bone marrow differentiation profiles of hematopoietic stem cells (HSCs), lymphocyte proliferation in various organs associated with the production of antibodies against DNA, myelin basic protein (MBP), and MOG, as well as abzymes hydrolyzing these antigens, were analyzed before and after immunization. Profiles of HSC differentiation [BFU-E (erythroid burst-forming unit (early erythroid colonies), CFU-E (erythroid burst-forming unit (late erythroid colonies), CFU-GM (granulocytic-macrophagic colony-forming unit), and CFU-GEMM granulocytic-erythroid-megakaryocytic-macrophagic colony-forming unit] and patterns of lymphocyte proliferation in different organs (brain, spleen, thymus, and lymph nodes) were very different for C57BL/6, CBA, and Th mice. We conclude that only C57BL/6 mice were predisposed to spontaneous and MOG-induced acceleration of EAE development. CBA mice are not prone to the development of autoimmune reactions. After immunization, Th mice demonstrate changes in several parameters similar to C57BL/6 and other to CBA mice; Th mice are more prone to developing autoimmune reactions than CBA mice. Our data may be important for understanding the combined presence in mice lymphocytes with T and B cell responses for spontaneous and induced autoimmune diseases.
Melatonin and Its Protective Role against Biotic Stress Impacts on Plants
Authors: Moustafa-Farag Mohamed , Almoneafy Abdulwareth , Mahmoud Ahmed , Elkelish Amr , Arnao Marino , Li Linfeng , Ai Shaoying
Published: December 2019

Biotic stress causes immense damage to agricultural products worldwide and raises the risk of hunger in many areas. Plants themselves tolerate biotic stresses via several pathways, including pathogen-associated molecular patterns (PAMPs), which trigger immunity and plant resistance (R) proteins. On the other hand, humans use several non-ecofriendly methods to control biotic stresses, such as chemical applications. Compared with chemical control, melatonin is an ecofriendly compound that is an economical alternative strategy which can be used to protect animals and plants from attacks via pathogens. In plants, the bactericidal capacity of melatonin was verified against Mycobacterium tuberculosis, as well as multidrug-resistant Gram-negative and -positive bacteria under in vitro conditions. Regarding plant–bacteria interaction, melatonin has presented effective antibacterial activities against phytobacterial pathogens. In plant–fungi interaction models, melatonin was found to play a key role in plant resistance to Botrytis cinerea, to increase fungicide susceptibility, and to reduce the stress tolerance of Phytophthora infestans. In plant–virus interaction models, melatonin not only efficiently eradicated apple stem grooving virus (ASGV) from apple shoots in vitro (making it useful for the production of virus-free plants) but also reduced tobacco mosaic virus (TMV) viral RNA and virus concentration in infected Nicotiana glutinosa and Solanum lycopersicum seedlings. Indeed, melatonin has unique advantages in plant growth regulation and increasing plant resistance effectiveness against different forms of biotic and abiotic stress. Although considerable work has been done regarding the role of melatonin in plant tolerance to abiotic stresses, its role in biotic stress remains unclear and requires clarification. In our review, we summarize the work that has been accomplished so far; highlight melatonin’s function in plant tolerance to pathogens such as bacteria, viruses, and fungi; and determine the direction required for future studies on this topic.
Decreased H19, GAS5, and linc0597 Expression and Association Analysis of Related Gene Polymorphisms in Rheumatoid Arthritis
Authors: Wu Jun , Zhang Tian-Ping , Zhao Yu-Lan , Li Bao-Zhu , Leng Rui-Xue , Pan Hai-Feng , Ye Dong-Qing
Published: December 2019

Long noncoding RNAs (lncRNAs) widely participate in human diseases by regulating gene transcription, modulating protein function, or acting as ceRNAs. Yet, their roles in rheumatoid arthritis (RA) remain obscure. In this study, the expression of three lncRNAs (H19, GAS5, and linc0597) in peripheral blood mononuclear cells (PBMCs) were detected in 77 RA patients and 78 controls using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The association of lncRNAs related gene polymorphisms with RA were evaluated in 828 RA patients and 780 controls using TaqMan single nucleotide polymorphism (SNP) genotyping assays. We observed that the expression levels of H19, GAS5 and linc0597 were down-regulated in PBMCs of RA patients, of which GAS5 level decreased in patients with hypocomplementemia, and negatively correlated with C-reactive protein (CRP) level in RA patients. Moreover, we highlighted two related potential functional SNPs, GAS5 rs6790 and linc0597 rs2680700 for associations with RA susceptibility. The precise roles of these lncRNAs in mechanism of RA remain to be further explored.
Anti-Atopic Effect of Acorn Shell Extract on Atopic Dermatitis-Like Lesions in Mice and Its Active Phytochemicals
Authors: Lee Sullim , Jegal Hyun , Bong Sim-Kyu , Yoon Kyeong-No , Park No-June , Shin Myoung-Sook , Yang Min Hye , Kim Yong Kee , Kim Su-Nam
Published: December 2019

To investigate the potential effects of acorn shells on atopic dermatitis (AD), we utilized oxazolone (OX)- or 2,4-dinitrochlorobenzene (DNCB)-induced AD-like lesion mouse models. Our research demonstrates that Acorn shell extract (ASE) improved the progression of AD-like lesions, including swelling, which were induced by oxazolone on Balb/c mouse ears. Additionally, ASE significantly decreased the ear thickness (OX: 0.42 ± 0.01 mm, OX-ASE: 0.32 ± 0.02 mm) and epidermal thickness (OX: 75.3 ± 32.6 µm, OX-ASE: 46.1 ± 13.4 µm). The continuous DNCB-induced AD mouse model in SKH-1 hairless mice demonstrated that ASE improved AD-like symptoms, including the recovery of skin barrier dysfunction, Immunoglobulin E hyperproduction (DNCB: 340.1 ± 66.8 ng/mL, DNCB-ASE: 234.8 ± 32.9 ng/mL) and an increase in epidermal thickness (DNCB: 96.4 ± 21.9 µm, DNCB-ASE: 52.4 ± 16.3 µm). In addition, we found that ASE suppressed the levels of AD-involved cytokines, such as Tumor Necrosis Factor α, IL-1β, IL-25 and IL-33 in both animal models. Furthermore, gallic acid and ellagic acid isolated from ASE suppressed β-hexosaminidase release and IL-4 expression in RBL-2H3 cells. The acorn shell and its active phytochemicals have potential as a new remedy for the improvement of atopic dermatitis and other inflammatory diseases.
Haptoglobin Hp1 Variant Does Not Associate with Small Vessel Disease
Authors: Lempiäinen Juha , Ijäs Petra , Niiranen Teemu J. , Kaste Markku , Karhunen Pekka J. , Lindsberg Perttu J. , Erkinjuntti Timo , Melkas Susanna
Published: December 2019

Haptoglobin (Hp) is a plasma protein that binds free hemoglobin and protects tissues from oxidative damage. An Hp2 allele has been associated with an increased risk of cardiovascular complications. On the other hand, recent studies have suggested that Hp1 allele increases risk to develop severe cerebral small vessel disease. We aimed to replicate this finding in a first-ever stroke patient cohort. Hp was genotyped by PCR and gel electrophoresis in the Helsinki Stroke Aging Memory Study in patients with DNA and magnetic resonance imaging (MRI) available (SAM; n = 316). Lacunar infarcts and white matter lesions (WML) classified by Fazekas grading from brain MRI were associated with Hp genotypes. As population controls, we used participants of Cardiovascular diseases—a sub study of Health 2000 Survey (n = 1417). In the SAM cohort, 63.0% of Hp1-1 carriers (n = 46), 52.5% of Hp1-2 carriers (n = 141) and 51.2% of Hp2-2 carriers (n = 129) had severe WML (p = 0.372). There was no difference in severe WMLs between Hp1-1 vs. Hp1-2 and Hp2-2 carriers (p = 0.201). In addition, 68.9% of Hp1-1 carriers (n = 45), 58.5% of Hp1-2 carriers (n = 135), and 61.8% of Hp2-2 carriers (n = 126) had one or more lacunar lesions (p = 0.472). There was no difference in the number of patients with at least one lacunar infarct between Hp1-1 vs. Hp1-2 and Hp2-2 groups (p = 0.322). Neither was there any difference when diabetic patients (type I and II) were examined separately. Hp1 allele is not associated with an increased risk for cerebral small vessel disease in a well-characterized Finnish stroke patient cohort.
Radiotherapy as a Backbone for Novel Concepts in Cancer Immunotherapy
Authors: Kabiljo Julijan , Harpain Felix , Carotta Sebastian , Bergmann Michael
Published: December 2019

Radiation-induced immunogenic cell death has been described to contribute to the efficacy of external beam radiotherapy in local treatment of solid tumors. It is well established that radiation therapy can induce immunogenic cell death in cancer cells under certain conditions. Initial clinical studies combining radiotherapy with immunotherapies suggest a synergistic potential of this approach. Improving our understanding of how radiation reconditions the tumor immune microenvironment should pave the way for designing rational and robust combinations with immunotherapeutic drugs that enhance both local and systemic anti-cancer immune effects. In this review, we summarize irradiation-induced types of immunogenic cell death and their effects on the tumor microenvironment. We discuss preclinical insights on mechanisms and benefits of combining radiotherapy with immunotherapy, focusing on immune checkpoint inhibitors. In addition, we elaborate how these observations were translated into clinical studies and which parameters may be optimized to achieve best results in future clinical trials.
Conversion Surgery in Metastatic Gastric Cancer and Cancer Dormancy as a Prognostic Biomarker
Authors: Choe Hun Jee , Kim Jin Won , Han Song-Hee , Lee Ju Hyun , Ahn Sang-Hoon , Park Do Joong , Kim Ji-Won , Kim Yu Jung , Lee Hye Seung , Kim Jee Hyun , Kim Hyung-Ho , Lee Keun-Wook
Published: December 2019

The role of conversion surgery in metastatic gastric cancer remains unclear. Cancer dormancy markers might have a role in predicting the survival in patients with conversion surgery. We identified 26 patients who went through conversion surgery, i.e., a curative-intent gastrectomy with metastasectomy after chemotherapy in initially metastatic gastric cancer. As controls, 114 potential candidates for conversion surgery who only received chemotherapy were included for the propensity score matching. Conversion surgery showed a significantly longer overall survival (OS) compared with only palliative chemotherapy (median—43.6 vs. 14.0 months, respectively, p < 0.001). This better survival in the conversion surgery group persisted even after propensity matching (p < 0.001), and also when compared to patients with tumor response over 5.1 months in the chemotherapy only group (p = 0.005). In the conversion surgery group, OS was longer in patients with R0 resection (22/26, 84.6%) than without R0 resection (4/26, 15.4%) (median—not reached vs 22.1 months, respectively, p = 0.005). Although it should be interpreted with caution due to the primitive analysis in a small population, the positive expression of NR2F1 showed a longer duration of disease-free survival (DFS) after conversion surgery (p = 0.016). In conclusion, conversion surgery showed a durable OS even in patients with initially metastatic gastric cancer when R0 resection was achieved after chemotherapy.
Bulk, Surface and Interface Promotion of Co3O4 for the Low-Temperature N2O Decomposition Catalysis
Authors: Wójcik Sylwia , Grzybek Gabriela , Stelmachowski Paweł , Sojka Zbigniew , Kotarba Andrzej
Published: December 2019

Nanocrystalline cobalt spinel has been recognized as a very active catalytic material for N2O decomposition. Its catalytic performance can be substantially modified by proper doping with alien cations with precise control of their loading and location (spinel surface, bulk, and spinel-dopant interface). Various doping scenarios for a rational design of the optimal catalyst for low-temperature N2O decomposition are analyzed in detail and the key reactivity descriptors are identified (content and topological localization of dopants, their redox vs. non-redox nature and catalyst work function). The obtained results are discussed in the broader context of the available literature data to establish general guidelines for the rational design of the N2O decomposition catalyst based on a cobalt spinel platform.
Transcriptional Suppression of the NLRP3 Inflammasome and Cytokine Release in Primary Macrophages by Low-Dose Anthracyclines
Authors: Köse-Vogel Nilay , Stengel Sven , Gardey Elena , Kirchberger-Tolstik Tatiana , Reuken Philipp A. , Stallmach Andreas , Bruns Tony
Published: December 2019

Tissue-resident macrophages play critical roles in controlling homeostasis, tissue repair, and immunity. Inflammatory macrophages can sustain tissue damage and promote the development of fibrosis during infections and sterile tissue injury. The NLRP3 inflammasome and its effector cytokine IL-1β have been identified as important mediators of fibrosis. Epirubicin, an anthracycline topoisomerase II inhibitor, has been reported to inhibit myeloid inflammatory cytokine production and to promote tissue tolerance following bacterial infection. We investigated the anti-inflammatory properties of epirubicin on the NLRP3 inflammasome and TLR4-mediated inflammation in PMA-primed THP-1 and in primary human peritoneal macrophages (PM). Low-dose epirubicin at non-cytotoxic doses downregulated NLRP3 inflammasome components and reduced the release of cleaved caspase-1, bioactive IL-1β, and TNF-α following NLRP3 activation in a dose-dependent fashion. In addition, epirubicin attenuated inflammatory macrophage responses after TLR4 and TLR2 ligation. These anti-inflammatory effects were not mediated by the induction of autophagy or altered MAPK signaling, but as the result of a global transcriptional suppression of LPS-dependent genes. Epirubicin-treated macrophages displayed reduced acetylation of histone 3 lysine 9 (H3K9ac), suggesting anti-inflammatory epigenetic imprinting as one underlying mechanism.
Investigation of Cuprizone-Induced Demyelination in mGFAP-Driven Conditional Transient Receptor Potential Ankyrin 1 (TRPA1) Receptor Knockout Mice
Authors: Kriszta Gábor , Nemes Balázs , Sándor Zoltán , Ács Péter , Komoly Sámuel , Berente Zoltán , Bölcskei Kata , Pintér Erika
Published: December 2019

Transient receptor potential ankyrin 1 (TRPA1) receptors are non-selective cation channels responsive to a variety of exogenous irritants and endogenous stimuli including products of oxidative stress. It is mainly expressed by primary sensory neurons; however, expression of TRPA1 by astrocytes and oligodendrocytes has recently been detected in the mouse brain. Genetic deletion of TRPA1 was shown to attenuate cuprizone-induced oligodendrocyte apoptosis and myelin loss in mice. In the present study we aimed at investigating mGFAP-Cre conditional TRPA1 knockout mice in the cuprizone model. These animals were generated by crossbreeding GFAP-Cre+/− and floxed TRPA1 (TRPA1Fl/Fl) mice. Cuprizone was administered for 6 weeks and demyelination was followed by magnetic resonance imaging (MRI). At the end of the treatment, demyelination and glial activation was also investigated by histological methods. The results of the MRI showed that demyelination was milder at weeks 3 and 4 in both homozygous (GFAP-Cre+/− TRPA1Fl/Fl) and heterozygous (GFAP-Cre+/− TRPA1Fl/−) conditional knockout animals compared to Cre−/− control mice. However, by week 6 of the treatment the difference was not detectable by either MRI or histological methods. In conclusion, TRPA1 receptors on astrocytes may transiently contribute to the demyelination induced by cuprizone, however, expression and function of TRPA1 receptors by other cells in the brain (oligodendrocytes, microglia, neurons) warrant further investigation.
Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors
Authors: Cutarelli Alessandro , Ghio Simone , Zasso Jacopo , Speccher Alessandra , Scarduelli Giorgina , Roccuzzo Michela , Crivellari Michele , Maria Pugno Nicola , Casarosa Simona , Boscardin Maurizio , Conti Luciano
Published: December 2019

Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, it is possible to build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems for human iPSC-derived cortical progenitors. In particular, our aim was to mimic the radially-oriented cortical radial glia fibres that during embryonic development play key roles in controlling the expansion, radial migration and differentiation of cortical progenitors, which are, in turn, pivotal to the establishment of the correct multilayered cerebral cortex structure. Here we show that silicon vertical micropillar arrays efficiently promote expansion and stemness preservation of human cortical progenitors when compared to standard monolayer growth conditions. Furthermore, the vertically-oriented micropillars allow the radial migration distinctive of cortical progenitors in vivo. These results indicate that vertical silicon micropillar arrays can offer an optimal system for human cortical progenitors’ growth and migration. Furthermore, similar structures present an attractive platform for cortical tissue engineering.
Tensin-3 Regulates Integrin-Mediated Proliferation and Differentiation of Tonsil-Derived Mesenchymal Stem Cells
Authors: Park Gi Cheol , Kim Hyung-Sik , Park Hee-Young , Seo Yoojin , Kim Ji Min , Shin Sung-Chan , Kwon Hyun-Keun , Sung Eui-Suk , Lee Jin-Choon , Lee Byung-Joo
Published: December 2019

Human palatine tonsils are potential tissue source of multipotent mesenchymal stem cells (MSCs). The proliferation rate of palatine tonsil-derived MSCs (TMSCs) is far higher than that of bone marrow-derived MSCs (BMSCs) or adipose tissue-derived MSCs (ADSCs). In our previous study, we had found through DNA microarray analysis that tensin-3 (TNS3), a type of focal adhesion protein, was more highly expressed in TMSCs than in both BMSCs and ADSCs. Here, the role of TNS3 in TMSCs and its relationship with integrin were investigated. TNS3 expression was significantly elevated in TMSCs than in other cell types. Cell growth curves revealed a significant decrease in the proliferation and migration of TMSCs treated with siRNA for TNS3 (siTNS3). siTNS3 treatment upregulated p16 and p21 levels and downregulated SOX2 expression and focal adhesion kinase, protein kinase B, and c-Jun N-terminal kinase phosphorylation. siTNS3 transfection significantly reduced adipogenic differentiation of TMSCs and slightly decreased osteogenic and chondrogenic differentiation. Furthermore, TNS3 inhibition reduced active integrin beta-1 (ITGβ1) expression, while total ITGβ1 expression was not affected. Inhibition of ITGβ1 expression in TMSCs by siRNA showed similar results observed in TNS3 inhibition. Thus, TNS3 may play an important role in TMSC proliferation and differentiation by regulating active ITGβ1 expression.
PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo
Authors: Yen Yu-Ting , Chien May , Lai Yung-Chih , Chen Dao-Peng , Chuong Cheng-Ming , Hung Mien-Chie , Hung Shih-Chieh
Published: December 2019

In most cancers, cellular origin and the contribution of intrinsic and extrinsic factors toward transformation remain elusive. Cell specific carcinogenesis models are currently unavailable. To investigate cellular origin in carcinogenesis, we developed a tumorigenesis model based on a combination of carcinogenesis and genetically engineered mouse models. We show in organoids that treatment of any of three carcinogens, DMBA, MNU, or PhIP, with protein phosphatase 2A (PP2A) knockout induced tumorigenesis in Lgr5+ intestinal lineage, but not in differentiated cells. These transformed cells increased in stem cell signature, were upregulated in EMT markers, and acquired tumorigenecity. A mechanistic approach demonstrated that tumorigenesis was dependent on Wnt, PI3K, and RAS-MAPK activation. In vivo combination with carcinogen and PP2A depletion also led to tumor formation. Using whole-exome sequencing, we demonstrate that these intestinal tumors display mutation landscape and core driver pathways resembling human intestinal tumor in The Cancer Genome Atlas (TCGA). These data provide a basis for understanding the interplay between extrinsic carcinogen and intrinsic genetic modification and suggest that PP2A functions as a tumor suppressor in intestine carcinogenesis.
Urinary Catheter Coating Modifications: The Race against Catheter-Associated Infections
Authors: Andersen Marissa J. , Flores-Mireles Ana L.
Published: December 2019

Urinary catheters are common medical devices, whose main function is to drain the bladder. Although they improve patients’ quality of life, catheter placement predisposes the patient to develop a catheter-associated urinary tract infection (CAUTI). The catheter is used by pathogens as a platform for colonization and biofilm formation, leading to bacteriuria and increasing the risk of developing secondary bloodstream infections. In an effort to prevent microbial colonization, several catheter modifications have been made ranging from introduction of antimicrobial compounds to antifouling coatings. In this review, we discuss the effectiveness of different coatings in preventing catheter colonization in vitro and in vivo, the challenges in fighting CAUTIs, and novel approaches targeting host–catheter–microbe interactions.
A Bayesian Model for Bivariate Causal Inference
Authors: Kurthen Maximilian , Enßlin Torsten
Published: December 2019

We address the problem of two-variable causal inference without intervention. This task is to infer an existing causal relation between two random variables, i.e., X → Y or Y → X , from purely observational data. As the option to modify a potential cause is not given in many situations, only structural properties of the data can be used to solve this ill-posed problem. We briefly review a number of state-of-the-art methods for this, including very recent ones. A novel inference method is introduced, Bayesian Causal Inference (BCI) which assumes a generative Bayesian hierarchical model to pursue the strategy of Bayesian model selection. In the adopted model, the distribution of the cause variable is given by a Poisson lognormal distribution, which allows to explicitly regard the discrete nature of datasets, correlations in the parameter spaces, as well as the variance of probability densities on logarithmic scales. We assume Fourier diagonal Field covariance operators. The model itself is restricted to use cases where a direct causal relation X → Y has to be decided against a relation Y → X , therefore we compare it other methods for this exact problem setting. The generative model assumed provides synthetic causal data for benchmarking our model in comparison to existing state-of-the-art models, namely LiNGAM, ANM-HSIC, ANM-MML, IGCI, and CGNN. We explore how well the above methods perform in case of high noise settings, strongly discretized data, and very sparse data. BCI performs generally reliably with synthetic data as well as with the real world TCEP benchmark set, with an accuracy comparable to state-of-the-art algorithms. We discuss directions for the future development of BCI.
Combined Stochastic Process and Value at Risk: A Real-World Information System Decision Case
Authors: Wu Liang-Chuan , Wu Liang-Hong , Pai Fan-Yun
Published: December 2019

In this study, we used a combined stochastic process and value-at-risk (VaR) method to examine an electronic commerce expansion decision. By modeling uncertain benefits as a stochastic process, maximum losses of alternative decisions were quantified and compared to help managers to make information system/information technology (IS/IT) project decisions. Our results, based on the maximum loss perspective, demonstrated that uncertainty plays a critical role in evaluating IS/IT projects. More importantly, the results illustrate that VaR serves as a useful tool in decision-making for managers to quantify the value of maximum possible loss and to help them reach decisions.
Productivity and Predictability for Measuring Morphological Complexity
Authors: Gutierrez-Vasques Ximena , Mijangos Victor
Published: December 2019

We propose a quantitative approach for quantifying morphological complexity of a language based on text. Several corpus-based methods have focused on measuring the different word forms that a language can produce. We take into account not only the productivity of morphological processes but also the predictability of those morphological processes. We use a language model that predicts the probability of sub-word sequences within a word; we calculate the entropy rate of this model and use it as a measure of predictability of the internal structure of words. Our results show that it is important to integrate these two dimensions when measuring morphological complexity, since languages can be complex under one measure but simpler under another one. We calculated the complexity measures in two different parallel corpora for a typologically diverse set of languages. Our approach is corpus-based and it does not require the use of linguistic annotated data.
A Comparison Analysis of BLE-Based Algorithms for Localization in Industrial Environments
Authors: Cannizzaro Davide , Zafiri Marina , Jahier Pagliari Daniele , Patti Edoardo , Macii Enrico , Poncino Massimo , Acquaviva Andrea
Published: December 2019

Proximity beacons are small, low-power devices capable of transmitting information at a limited distance via Bluetooth low energy protocol. These beacons are typically used to broadcast small amounts of location-dependent data (e.g., advertisements) or to detect nearby objects. However, researchers have shown that beacons can also be used for indoor localization converting the received signal strength indication (RSSI) to distance information. In this work, we study the effectiveness of proximity beacons for accurately locating objects within a manufacturing plant by performing extensive experiments in a real industrial environment. To this purpose, we compare localization algorithms based either on trilateration or environment fingerprinting combined with a machine-learning based regressor (k-nearest neighbors, support-vector machines, or multi-layer perceptron). Each algorithm is analyzed in two different types of industrial environments. For each environment, various configurations are explored, where a configuration is characterized by the number of beacons per square meter and the density of fingerprint points. In addition, the fingerprinting approach is based on a preliminary site characterization; it may lead to location errors in the presence of environment variations (e.g., movements of large objects). For this reason, the robustness of fingerprinting algorithms against such variations is also assessed. Our results show that fingerprint solutions outperform trilateration, showing also a good resilience to environmental variations. Given the similar error obtained by all three fingerprint approaches, we conclude that k-NN is the preferable algorithm due to its simple deployment and low number of hyper-parameters.
Phase-Shift PWM Converter with Wide Voltage Operation Capability
Authors: Lin Bor-Ren
Published: December 2019

A soft switching three-level pulse-width modulation (PWM) converter is presented for industrial electronics with wide voltage range operation, such as solar power or fuel cell applications. Phase shift PWM scheme is used on the input-side to accomplish the zero voltage turn-on on power switches and improve the converter efficiency. Three-level diode-clamp circuit topology is adopted in the presented circuit to lessen the voltage ratings on active devices for high voltage applications. Three sub-circuits with the different turns-ratio of transformers can be selected in the presented converter in order to achieve 10:1 (Vin,max = 10Vin,min) wide input voltage operation when compared to the conventional multilevel converter. The proposed circuit is a single-stage converter instead of two-stage converter to realize wide voltage operation. Therefore, the presented converter has less component counts. Finally, the design procedure and experiments with a 300W laboratory circuit are presented and discussed to confirm the circuit analysis and converter performance.
Design and Implementation of 150 W AC/DC LED Driver with Unity Power Factor, Low THD, and Dimming Capability
Authors: Tung Ngo Thanh , Tuyen Nguyen Dinh , Huy Nguyen Minh , Phong Nguyen Hoai , Cuong Ngo Cao , Phuong Le Minh
Published: December 2019

This paper presents the implementation of a two-stage light-emitting diode (LED) driver based on commercial integrated circuits (IC). The presented LED driver circuit topology, which is designed to drive a 150 W LED module, consists of two stages: AC-DC power factor correction (PFC) stage and DC/DC power converter stage. The implementation of the PFC stage uses IC NCP1608, which uses the critical conduction mode to guarantee a unity input power factor with a wide range of input voltages. The DC/DC power converter with soft-switching characteristics for the entire load range uses IC FLS2100XS. Furthermore, the design of an electromagnetic interference (EMI) filter for the LED driver and the dimming control circuit are discussed in detail. The hardware prototype, an LED lighting system, with a rated power of 150 W/32 V from a nominal 220 V/50 Hz AC voltage supply was tested to show the effectiveness of the design. The presented LED driver was tested for street lighting, and the experimental results show that the power factor (PF) was higher than 0.97, the total harmonics distortion (THD) was lower than 7%, and the efficiency was 91.7% at full load. The results prove that the performance of the presented LED driver complies with the standards: IEC61000-3-2 and CIRSP 15:2009.
Potable Reuse of Coalbed Methane-Produced Waters in Developing Country Contexts—Could the Benefits Outweigh the Costs to Facilitate Coal Transitions?
Authors: Singh Udayan , Colosi Lisa M.
Published: December 2019

Development of coalbed methane (CBM) projects is critical to the achievement of climate change goals because it will help facilitate coal-to-gas transitions in Asia-Pacific countries with low conventional gas reserves. However, growth in CBM in these regions will necessitate strategic, sustainable approaches to produced water management. We posit that it may be possible to deliver synergistic water, energy, and health benefits by reusing CBM-produced waters as potable water supply in water-stressed coal-bearing regions. The goal of this study is to probabilistically evaluate life cycle costs and benefits of using reverse osmosis to treat CBM-produced water in the Damodar Valley coalfields in eastern India. Two treatment configurations are assessed, namely, centralized, and decentralized (i.e., in-home). We find that both configurations offer good cost-effectiveness based on two separately computed metrics to account for the value of health improvement benefits (i.e., disability-adjusted life years (DALYs) averted or monetized health benefits). We also observe that centralized systems are more cost-effective than decentralized, because they reduce capital cost and use-phase energy consumption per unit-volume treated. Average estimated values for the cost–benefit ratio are <0.5 and 1.0 for centralized and decentralized, respectively. Normalizing by anticipated health benefits, cost-effectiveness metrics are <$30/DALY for the centralized system versus <$200/DALY for the decentralized system. These results are highly sensitive to the value of statistical life and baseline water access. A related analysis taking into account both CBM-produced waters and mine waters revealed that deployment of reverse osmosis (RO) could provide drinking to approximately 3.5 million people over 20 years in the Damodar Valley region. These results have interesting implications not only for the study region but also for other CBM-producing countries experiencing chronic severe water stress.
Life-Cycle Assessment (LCA) of Different Pretreatment and Product Separation Technologies for Butanol Bioprocessing from Oil Palm Frond
Authors: Mahmud Nazira , Rosentrater Kurt A.
Published: December 2019

Environmental impact assessment is a crucial aspect of biofuels production to ensure that the process generates emissions within the designated limits. In typical cellulosic biofuel production process, the pretreatment and downstream processing stages were reported to require a high amount of chemicals and energy, thus generating high emissions. Cellulosic butanol production while using low moisture anhydrous ammonia (LMAA) pretreatment was expected to have a low chemical, water, and energy footprint, especially when the process was combined with more efficient downstream processing technologies. In this study, the quantification of environmental impact potentials from cellulosic butanol production plants was conducted with modeled different pretreatment and product separation approaches. The results have shown that LMAA pretreatment possessed a potential for commercialization by having low energy requirements when compared to the other modeled pretreatments. With high safety measures that reduce the possibility of anhydrous ammonia leaking to the air, LMAA pretreatment resulted in GWP of 5.72 kg CO2 eq./L butanol, ecotoxicity potential of 2.84 × 10−6 CTU eco/L butanol, and eutrophication potential of 0.011 kg N eq./L butanol. The lowest energy requirement in biobutanol production (19.43 MJ/L), as well as better life-cycle energy metrics performances (NEV of 24.69 MJ/L and NER of 2.27) and environmental impacts potentials (GWP of 3.92 kg N eq./L butanol and ecotoxicity potential of 2.14 × 10−4 CTU eco/L butanol), were recorded when the LMAA pretreatment was combined with the membrane pervaporation process in the product separation stage.
Multi-Criteria Decision Analysis of Road Transportation Fuels and Vehicles: A Systematic Review and Classification of the Literature
Authors: Kügemann Martin , Polatidis Heracles
Published: December 2019

Multi-Criteria Decision Analysis (MCDA) methods help decision makers to consider and weigh diverse criteria that include economic, environmental, social and technological aspects. This characteristic makes them a popular tool to comparatively evaluate road transportation fuels and vehicles (RTFV). The aim of this paper is to systematically classify and analyse the literature applying MCDA methods on the evaluation of RTFV. To this end, 40 relevant papers are pinpointed and discussed. We identified a great number of evaluation criteria employed in the reviewed papers from which we have established a concluding list of 41 criteria, that can serve as a pool for future research. A further analysis of the evaluation criteria reveals that the process of criteria selection partly suffers from a lack of scientific foundation and standardization. We propose to standardize the criteria selection process by using the Life Cycle Sustainability Assessment (LCSA) methodology as a guiding reference. In addition, we compared the MCDA results obtained from studies with relatively similar setups and found that the evaluation results are also generally similar and seem not to be influenced by the particular MCDA method employed. Based on the results of the reviewed papers, one may say that electricity and ethanol appear to be good alternatives for light vehicles, whereas gaseous fuels seem more appropriate for heavy vehicles like buses. Striking deviations from these generally observed results are often caused by specific evaluation contexts, particular criteria taken into account and unusual weight sets applied.
Voltage Regulation Planning for Distribution Networks Using Multi-Scenario Three-Phase Optimal Power Flow
Authors: Baran Junior Antonio Rubens , Piazza Fernandes Thelma S. , Borba Ricardo Augusto
Published: December 2019

Active distribution networks must operate properly for different scenarios of load levels and distributed generation. An important operational requirement is to maintain the voltage profile within standard operating limits. To do this, this paper proposed a Multi-Scenario Three-Phase Optimal Power Flow (MTOPF) that plans the voltage regulation of unbalance and active distribution networks considering typical scenarios of operation. This MTOPF finds viable operation points by the optimal adjustments of voltage regulator taps and distribution transformer taps. The differentiating characteristic of this formulation is that in addition to the traditional tuning of voltage regulator taps of an active network applied for just one scenario of load and generation, it also performs the optimal adjustment of distribution transformer taps, which, once fixed, is able to meet the voltage limits of diverse operating situations. The optimization problem was solved by the primal-dual interior-point method and the formulation was tested using the IEEE 123-bus system.
Experimental Study of Nucleate Boiling of Flammable, Environmentally Friendly Refrigerants
Authors: Gil Bartosz , Fijałkowska Beata
Published: December 2019

This paper investigates the nucleate boiling process of dimethyl ether and selected hydrocarbons. The main goal of this study is to measure the heat transfer coefficients of RE170, R600a, and R601, and to compare them with R134a. The experiments were carried out for heat fluxes up to 70 kW/m2. Experimental results have shown a typical trend that the heat transfer coefficient of flammable refrigerants increases as the heat flux increases. Among the tested fluids, the highest values of heat transfer coefficient were obtained for RE170. Available correlations describing this coefficient showed a deviation of up to 93%, as compared to the data obtained. The new correlation was developed by regression analysis taking into account dimensionless variables affecting the boiling process.
Analysis of the Thermal Behavior of an Earthbag Building in Mediterranean Continental Climate: Monitoring and Simulation
Authors: Rincón Lídia , Carrobé Ariadna , Medrano Marc , Solé Cristian , Castell Albert , Martorell Ingrid
Published: December 2019

Nearly 30% of humanity lives in earthen dwellings. Earthbag is a sustainable, cheap, feasible and comfortable option for emergency housing. A comparative monitoring-simulation analysis of the hygrothermal behavior of an Earthbag dwelling in Mediterranean continental climate, designed under bioclimatic criteria, is presented. The dome shape Earthbag dwelling has a net floor area of 7.07 m2, a glass door facing south and two confronted windows in the east and west facades. A numerical model (EnergyPlus v8.8) was designed for comparison. Twenty-four hour cross ventilation, night cross ventilation, and no ventilation in free floating mode and a controlled indoor temperature were the tested scenarios. Comparisons between experimental data and simulation show a good match in temperature behavior for the scenarios studied. Reductions of 90% in summer and 88% in winter, in the interior thermal amplitude with respect to exterior temperatures are found. Position of the glazed openings was fundamental in the direct solar gains, contributing to the increase of temperature in 1.31 °C in winter and 1.37 °C in the equinox. Night ventilation in the summer period had a good performance as a passive system. Passive solar gains made a reduction of heating energy consumption of 2.3% in winter and 8.9% in equinox.
Analytical and Numerical Feasibility Analysis of a Contra-Rotary Ramjet Engine
Authors: Laube Tomasz , Piechna Janusz
Published: December 2019

A new idea for a contra-rotary ramjet engine is presented. To define the theoretical limits of the non-typical, contra-rotary ramjet engine configuration, its analytical model was developed. The results obtained from that model and the analytical results were compared with those received from numerical simulations. The main weakness of existing rotary ramjet engine projects is the very high rotational speed of the rotor required for achieving supersonic inlet flow. In this paper, a new idea for a contra-rotary ramjet engine (CORRE) is presented and analyzed. This paper presents the results of analytical analysis and numerical simulations of a jet engine system with two rotors rotating in opposite directions. Contra-rotating rotors generate a supersonic air velocity at the inlet to the compressor at two times slower rotor’s speed. To determine the flow characteristics, combustion process, and engine efficiency of the double-rotor engine, a numerical solution of the average Navier-Stokes equations was used with the k-eps turbulence model and the non-premixed combustion model. The results of numerical simulations of flow and the combustion process inside the contra-rotary jet engine achieving a shockwave compression are shown and compared with similar data for a single-rotor engine design and analytical data. This paper presents only the calculation results of the flow processes and the combustion process, indicating the advantages of the proposed double-rotor design. The results of the numerical analysis were presented on the contours and diagrams of the pressure and flow velocity, temperature distribution, and mass fraction of the fuel.
The Impact of Freeze-Drying Conditions on the Physico-Chemical Properties and Bioactive Compounds of a Freeze-Dried Orange Puree
Authors: Silva-Espinoza Marilú A. , Ayed Charfedinne , Foster Timothy , Camacho María del Mar , Martínez-Navarrete Nuria
Published: December 2019

Fruits are essential for a healthy diet, as they contribute to the prevention of cardiovascular diseases and some cancers, which is attributed to their high bioactive compound content contributing to their antioxidant capacity. Nevertheless, fruits have a short shelf life due to their high-water content, and freeze-drying is a well-known technique to preserve their nutritive quality. However, it is an expensive technology, both due to the use of low pressure and long processing time. Therefore, an optimisation of variables such as the freezing rate, working pressure and shelf temperature during freeze-drying may preserve fruit quality while reducing the time and costs. The impact of these variables on colour, porosity, mechanical properties, water content, vitamin C, total phenols, β-carotene, and antioxidant activity of a freeze-dried orange puree was evaluated. The results showed a great impact of pressure and shelf temperature on luminosity, chroma and water content. Vitamin C and β-carotene were more preserved with higher shelf temperatures (shorter times of processing) and lower pressure, respectively. The optimum freeze-drying conditions preserving the nutrients, and with an interesting structural property, perceived as a crunchy product by consumers, are low pressure (5 Pa) and high shelf temperature (50 °C).
Genome-Wide Analysis of the NAC Domain Transcription Factor Gene Family in Theobroma cacao
Authors: Shen Shiya , Zhang Qianru , Shi Yu , Sun Zhenmei , Zhang Qianqian , Hou Sijia , Wu Rongling , Jiang Libo , Zhao Xiyang , Guo Yunqian
Published: December 2019

As a plant-specific transcription factor, the NAC (NAM, ATAF1/2 and CUC2) domain protein plays an important role in plant growth and development, as well as stress resistance. Based on the genomic data of the cacao tree, this study identified 102 cacao NAC genes and named them according to their location within the genome. The phylogeny of the protein sequence of the cacao tree NAC family was analyzed using various bioinformatic methods, and then divided into 12 subfamilies. Then, the amino-acid composition, physicochemical properties, genomic location, gene structure, conserved domains, and promoter cis-acting elements were analyzed. This study provides information on the evolution of the TcNAC gene and its possible functions, laying the foundation for further research on the NAC family.
Construction of High-Density Genetic Map and Identification of QTLs Associated with Seed Vigor after Exposure to Artificial Aging Conditions in Sweet Corn Using SLAF-seq
Authors: Wu Xiaming , Feng Faqiang , Zhu Yuzhong , Xie Fugui , Yang Jing , Gong Jie , Liu Yu , Zhu Wei , Gao Tianle , Chen Danyi , Li Xiaoqin , Huang Jun
Published: December 2019

Seed vigor is a key factor that determines the quality of seeds, which is of great significance for agricultural production, with the potential to promote growth and productivity. However, the underlying molecular mechanisms and genetic basis for seed vigor remain unknown. High-density genetic linkage mapping is an effective method for genomic study and quantitative trait loci (QTL) mapping. In this study, a high-density genetic map was constructed from a 148 BC4F3 population cross between ‘M03’ and ‘M08’ strains based on specific-locus amplified fragment (SLAF) sequencing. The constructed high-density genetic linkage map (HDGM) included 3876 SNP markers on ten chromosomes covering 2413.25 cM in length, with a mean distance between markers of 0.62 cM. QTL analysis was performed on four sweet corn germination traits that are related to seed vigor under artificial aging conditions. A total of 18 QTLs were identified in two seasons. Interestingly, a stable QTL was detected in two seasons on chromosome 10—termed qGR10—within an interval of 1.37 Mb. Within this interval, combined with gene annotation, we found four candidate genes (GRMZM2G074309, GRMZM2G117319, GRMZM2G465812, and GRMZM2G343519) which may be related to seed vigor after artificial aging.
Biomphalaria glabrata Granulin Increases Resistance to Schistosoma mansoni Infection in Several Biomphalaria Species and Induces the Production of Reactive Oxygen Species by Haemocytes
Authors: Hambrook Jacob R. , Gharamah Abdullah A. , Pila Emmanuel A. , Hussein Solomon , Hanington Patrick C.
Published: December 2019

Gastropod molluscs, which have co-evolved with parasitic digenean trematodes for millions of years, utilize circulating heamocytes as the primary method of containing and killing these invading parasites. In order to do so, they must generate suitable amounts of haemocytes that are properly armed to kill parasitic worms. One method by which they generate the haemocytes required to initiate the appropriate cell mediated immune response is via the production and post-translational processing of granulins. Granulins are an evolutionarily conserved family of growth factors present in the majority of eukaryotic life forms. In their pro-granulin form, they can elicit cellular replication and differentiation. The pro-granulins can be further processed by elastase to generate smaller granulin fragments that have been shown to functionally differ from the pro-granulin precursor. In this study, we demonstrate that in vivo addition of Biomphalaria glabrata pro-granulin (BgGRN) can reduce Schistosoma mansoni infection success in numerous Biomphalaria sp. when challenged with different S. mansoni strains. We also demonstrate that cleavage of BgGRN into individual granulin subunits by elastase results in the stimulation of haemocytes to produce reactive oxygen species.