bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a Community-Driven Waste Reduction Education and Action Program
Authors: Chenier Keith A. , Englebretson Elizabeth , James-Barry Jessi A. , Rigsby Amanda N. , Rodolfich Alyssa E. , McQueen Evelyn P. , Sparks Eric L.
Published: June 2024

Mismanaged and excessive waste has adverse effects on natural environments, economies, and human health. To address these waste issues, the Sustainability with Awareness and Prevention Solutions Program was developed and implemented in communities along the Mississippi Gulf Coast (USA) with the aim to provide the knowledge and actions needed to reduce or eliminate mismanaged and excessive waste. The program uses four educational modules focused on (1) microplastics in the watershed, (2) food waste, (3) single-use plastics, and (4) involvement in community prevention and removal programs. The program curriculum was written to meet education standards in Mississippi for 5th grade gifted, 6–8th grades, and 9–12th grades, and can be easily adapted to other states’ curriculums. The program provides material incentives to participants so knowledge learned can immediately be translated to behavior change. The program is continually being developed to find the best methods to implement behavior change at multiple scales.
Exploring the Relationships among HRM Investment, Strategy Implementation, and Firm Performance with Multiple Correspondence Analysis
Authors: Choo Seungyoup
Published: June 2024

This study visually explores the relationships among human resource management (HRM) investment, strategy implementation, and firm performance for small- and medium-sized enterprises (SMEs) in Korea. This exploratory research focuses on the significance of strategy implementation, an often overlooked factor in the relationship between HRM and firm performance, as well as HRM investment, which can enable the development of organizational capabilities. We conducted a multiple correspondence analysis to understand the interrelationship between HRM investment, competitive strategy implementation, and firm performance. We differentiated the results by group type according to the level of HRM investment, competitive strategy implementation, and firm performance. We found that firms with high HRM investment achieve the highest performance by implementing an ambidextrous strategy that simultaneously pursues cost leadership and differentiation. Ultimately, this study’s results suggest that for firms to create a competitive advantage through the strategic capitalization of human resources, long-term and continuous investment in HRM is necessary, even if short-term visible effects are not observed while the HRM system is being built, to eventually establish the organization’s capacity to support strategy execution. This study’s potential contribution is to extend the resource-based view by establishing the role of strategy implementation in linking HRM systems as organizational capabilities to performance.
Solar Powered Thermoelectric Air Conditioning for Temperature Control in Poultry Incubators
Authors: Cheepati Kumar Reddy , Balal Nezah
Published: June 2024

Proper air conditioning is crucial for hatching, growing, and reproducing poultry chickens. The existing methods are often costly and only viable for some chicken farmers. This paper presents a novel solar-powered thermoelectric module that utilizes the Peltier effect for efficient cooling and heating in poultry incubators. The proposed system consists of a Peltier module with cool and hot junctions, powered by a solar panel through a charge controller and battery. The cool junction is located in the chicken-breeding and reproduction unit, while the hot junction is situated in the egg-incubation unit. Temperature controllers maintain the required temperatures of 35–40 °C for the egg-hatching and 10–24 °C for the chicken-growing units. The experimental results demonstrate the system’s effectiveness in maintaining the desired temperatures. This solar-powered thermoelectric air conditioning system offers advantages over traditional methods, including lower energy consumption, reduced costs, and eco-friendliness. It has the potential to benefit off-grid poultry farmers and reduce energy bills for existing chicken farms. The mathematical modeling, load calculations, and prototype results show that the proposed system is best suited for providing the required cooling and heating effects in poultry incubators. This research represents a significant step forward in temperature control for poultry incubators and could revolutionize poultry farming practices, especially in remote locations with limited electricity access.
Evaluating Logistics Companies’ Readiness towards Adopting Synchromodality in the Flanders Region
Authors: Alaei Shafagh , Mommens Koen , Durán-Micco Javier , Macharis Cathy
Published: June 2024

The transport sector accounts for approximately a quarter of the EU’s total greenhouse gas emissions, with freight transport alone accounting for about one-third of the overall transport-related emissions. Mitigating the sector’s environmental impact is crucial for tackling climate change and achieving sustainable development goals. Modal shift is one of the main solutions to address this challenge; however, many companies have yet to realize its full potential. This paper presents a survey conducted in the Flanders region of Belgium, aiming to identify the challenges and barriers faced by industry players in this key geographical area and to explore the reasons behind the limited implementation of synchromodal transport among them. The survey evaluates the current state of synchromodal transport adoption and offers valuable insights for policymakers and industry stakeholders aiming to enhance sustainability in the logistics sector. The findings emphasize that to overcome the identified challenges, both policy support and the companies’ commitment are required. Policy support includes establishing consistent regulations and promoting greener transport modes through providing incentives and technological advancements. This research contributes to the field by examining barriers to the adoption of synchromodality and exploring its application within the context of Flanders. By focusing on this strategic logistics hub, the study provides insights and recommendations tailored to the specific challenges of the region’s logistics sector. The challenges faced by industry players in Flanders offer a deeper understanding of modal shift dynamics, facilitating informed decision-making for policymakers and industry stakeholders. Implementing these strategies paves the way for more environmentally friendly, efficient, and integrated transport, benefiting both the industry and the planet.
The Importance of the Product “Tourism in Bullfighting Ranches” in Spain from the Perspective of the Breeders
Authors: Flores-García Paloma , Sánchez-Martín José Manuel , Rengifo-Gallego Juan Ignacio
Published: June 2024

Bullfighting tourism is a recent tourism modality that is in constant growth, and which consists of visits to bullfighting ranches with the aim of showing what life is like for this animal in its natural habitat. This activity represents a source of extra income for many breeders, but it has not yet been adequately promoted and publicized. This study analyzes the supply side of this type of tourism. For this purpose, a non-parametric statistical model has been used and results have been obtained which corroborate that the promotion of this type of tourism is scarce, and that the breeders began to offer this type of tourism to make known what life is like for the fighting bull in the countryside and to increase their income. Furthermore, in this study, it is fundamental to differentiate between bullfighting and bullfighting tourism so that this type of tourism can reach the greatest possible number of people and not be influenced by thoughts against bullfighting, as they are different activities.
Deploying Bottleneck Management Strategies for Ameliorating Critical Delays in Building Construction Projects: A Case for Developing Country of Iran
Authors: Karimi Hamidreza , Sarvari Hadi , Edwards David J. , Chan Daniel W. M. , Olawumi Timothy O.
Published: June 2024

One of the primary concerns and challenges encountered in the construction industry is the emergence of crucial factors instigating project delays throughout the construction project lifecycle (CPL). The critical delay factors (CDFs) are the significant factors that not only cause project delays but also create obstacles and bottlenecks for the projects. Hence, the current study aims to determine CDFs affecting project completions and ameliorates the adverse situation by developing relevant bottleneck management strategies. To achieve this goal, a desktop review of previous research studies was undertaken to identify the CDFs in the CPL. The brainstorming technique was further utilized to filter the identified CDFs and match them to the context of developing countries, using Iran as a case example. Finally, an empirical questionnaire was created that included 22 CDFs divided into three distinct groups. The questionnaire’s validity and reliability were checked and validated before massive distribution to target respondents. Sixty industry experts appraised the identified CDFs in the CPL based on two assessment criteria: the severity of impact and probability of occurrence. The findings revealed that the groups with the most significant level of impact (out of 5 points) are project planning and design (2.29), construction and delivery (1.99), and policymaking and legislation (1.72). Similarly, the groups of project planning and design (2.30), construction and delivery (2.20), and policymaking and legislation (1.5) were ranked from first to third based on the probability of occurrence. According to the survey findings, the project planning and design stage is the most optimal time to mitigate the impact of project delays. Moreover, the study posited some pragmatic recommendations as bottleneck management strategies for ameliorating the identified CDFs for future projects. The study deliverables can serve as an effective tool for project stakeholders and decision makers to diminish the impact on and penetration of CDFs into building construction projects and enhance the delivery path leading to project success.
Potential of MMP-2 and MMP-9 Gelatinase Blockade as a Therapeutic Strategy in Fibrosarcoma Treatment: A Decadal Review
Authors: Shoari Alireza
Published: June 2024

Fibrosarcoma represents a significant challenge in oncology, characterized by high invasiveness and a poor prognosis. Gelatinases, particularly matrix metalloproteinases MMP-2 and MMP-9, play a pivotal role in the degradation of the extracellular matrix, facilitating tumor invasion and metastasis. Inhibiting these enzymes has emerged as a promising therapeutic strategy. This review evaluates the progress in the development and therapeutic potential of gelatinase inhibitors as treatments for fibrosarcoma over the last decade, highlighting molecular mechanisms and future directions. A comprehensive literature review was conducted, focusing on studies published from 2013 to 2023. Research articles and review papers relevant to gelatinase inhibition and fibrosarcoma were examined to assess the efficacy and mechanisms of gelatinase inhibitors. Gelatinase inhibitors have shown the potential to reduce tumor progression, invasion, and metastasis in fibrosarcoma. Clinical trials, although limited, have indicated that these inhibitors can be effectively integrated into existing therapeutic regimens, offering a reduction in metastatic spread and potentially improving patient survival rates. Mechanistic studies suggest that the inhibition of MMP-2 and MMP-9 disrupts critical pathways involved in tumor growth and cell invasion. Gelatinase inhibition represents a viable and promising approach to fibrosarcoma treatment. Future research should focus on developing more specific inhibitors, understanding long-term outcomes, and integrating gelatinase inhibition into multimodal treatment strategies to enhance efficacy.
Antibiotics in Surface Sediments from the Anning River in Sichuan Province, China: Occurrence, Distribution, and Risk Assessment
Authors: Zhou Junlie , Kang Jianglin , Lin Chunyan , Xu Qi , Yang Wanrong , Fan Ke , Li Jinrong
Published: June 2024

The occurrence, distribution, and ecological risk assessment of 36 antibiotics from five groups, including macrolides (MLs), fluoroquinolones (FQs), tetracyclines (TCs), amphenicols (APs), and sulfonamides (SAs), were investigated for the first time in the Anning River, Sichuan Province, China. The results show that antibiotics were widely present in the sediments of the Anning River, with a total of 22 antibiotics detected. FQs were among the most abundant antibiotics, followed by TCs, MLs, APs, and SAs. The total concentrations of antibiotics in surface sediments varied from 0.05 to 53.35 ng/g, with an average of 8.09 ng/g. Among these groups, MLs, FQs, and TCs emerged as the predominant classes of antibiotics. The midstream sediments showed the highest residual levels of antibiotics, with lower levels observed in the downstream and upstream sediments. Anthropogenic activities, such as human clinical practices and animal breeding, might be sources of antibiotics released into the river. An ecological risk assessment revealed that trimethoprim from the SA group exhibited high risks, and MLs showed medium risks in the Anning River, whereas most antibiotics presented minimal to low risks. This study provides valuable information on antibiotic pollution in the upstream region of the Yangtze River, and future management measures are needed for the Anning River.
Biogas Digestate and Sewage Sludge as Suitable Feeds for Black Soldier Fly (Hermetia illucens) Larvae
Authors: Kofroňová Jana , Melliti Abir , Vurm Radek
Published: June 2024

Hermetia illucens larvae can use organic wastes as a substrate, which makes them an interesting potential feed. However, waste may contain heavy metals, which are limited in feed. Here, we investigated the ability of H. illucens to grow on organic wastes and measured their heavy metal bioaccumulation. The larvae were fed with food waste, biogas digestates, and sewage sludge. When the first adult fly was visible, the tests were stopped and the larvae immediately processed. The samples (wastes before use, larvae after feeding) were analysed for mineral nutrient and heavy metal content using AAS and ICP-OES, respectively. The results show that the weight of the larvae fed with food waste increased sevenfold, which was broadly in line with expectations. Those fed with sewage sludge and digestate from biogas station increased threefold. While the larvae fed with sewage sludge exceeded the limits for heavy metals, particularly Cd and Pb, in feedstock, those fed with biogas digestate and food waste did not. These findings add to the literature showing the suitability of different wastes as H. illucens feed, and the importance of excluding waste contaminated with heavy metals from larvae intended for use as animal feed, or else diverting these larvae to non-feed uses.
Constraints on the Primordial Curvature Power Spectrum and Reheating Temperature from the NANOGrav 15-Year Dataset
Authors: Fei Qin
Published: June 2024

The stochastic signal observed by collaborations such as NANOGrav, PPTA, EPTA +InPTA, and CPTA may originate from gravitational waves induced by primordial curvature perturbations during inflation. This study investigates small-scale properties of inflation and reheating, assuming a log-normal form for the power spectrum of the primordial curvature and a reheating phase equation of state w=1/9. Inflation and reheating scenarios are thoroughly examined using Bayesian methods applied to the NANOGrav 15-year dataset. The analysis establishes constraints on the reheating temperature, suggesting Trh≳0.1Gev, consistent with Big Bang nucleosynthesis constraints. Additionally, the NANOGrav 15-year dataset requires the amplitude (A∼0.1) and width (Δ≲0.001) of the primordial curvature power spectrum to be within specific ranges. A notable turning point in the energy density of scalar-induced gravitational waves occurs due to a change in the equation of state w. This turning point signifies a transition from the reheating epoch to radiation domination. Further observations of scalar-induced gravitational waves could provide insights into the precise timing of this transition, enhancing our understanding of early Universe dynamics.
Improving Green Literacy and Environmental Culture Associated with Youth Participation in the Circular Economy: A Case Study of Vietnam
Authors: Tran Phuong Mai , Nguyen Thuy , Nguyen Huu-Dung , Thinh Nguyen An , Lam Nguyen Duc , Huyen Nguyen Thi , Khuc Van Quy
Published: June 2024

The circular economy (CE), a sustainability concept that promotes resource efficiency and waste reduction, has garnered significant popularity in recent years due to its potential to address pressing environmental and economic challenges. This study applies the Bayesian Mindsponge Mindspongeconomics (BMM) framework/analytic method, based on the Bayesian Mindsponge Framework (BMF), to the factors influencing young adults’ pro-environmental behavior and their purchases of green products at different price levels. The findings indicate that young adults who are knowledgeable about the CE and who value environmental protection and energy conservation are more likely to engage in waste sorting, while the factors that affect their willingness to pay (WTP) more for green and energy-saving products vary at different price tiers. This study demonstrates that knowledge of the CE, daily waste sorting habits, and environmental concern positively impact young adults’ WTP for products that are priced 5%, 10%, and 15% higher, respectively. Furthermore, this study also highlights the potential of educational programs and cultural influences in nurturing a generation that prioritizes environmental value. This research integrates multidisciplinary perspectives and offers practical implications for policymakers, educators, and businesses seeking to promote green literacy and foster an environmental culture among the youth, contributing to the broader goals of green transformation and sustainable development associated with the CE and the green economy, especially in the urban areas of emerging countries and beyond.
Coxsackievirus A7 and Enterovirus A71 Significantly Reduce SARS-CoV-2 Infection in Cell and Animal Models
Authors: Svyatchenko Victor A. , Legostaev Stanislav S. , Lutkovskiy Roman Y. , Protopopova Elena V. , Ponomareva Eugenia P. , Omigov Vladimir V. , Taranov Oleg S. , Ternovoi Vladimir A. , Agafonov Alexander P. , Loktev Valery B.
Published: June 2024

In this study, we investigated the features of co-infection with SARS-CoV-2 and the enterovirus vaccine strain LEV8 of coxsackievirus A7 or enterovirus A71 for Vero E6 cells and Syrian hamsters. The investigation of co-infection with SARS-CoV-2 and LEV-8 or EV-A71 in the cell model showed that a competitive inhibitory effect for these viruses was especially significant against SARS-CoV-2. Pre-infection with enteroviruses in the animals caused more than a 100-fold decrease in the levels of SARS-CoV-2 virus replication in the respiratory tract and more rapid clearance of infectious SARS-CoV-2 from the lower respiratory tract. Co-infection with SARS-CoV-2 and LEV-8 or EV-A71 also reduced the severity of clinical manifestations of the SARS-CoV-2 infection in the animals. Additionally, the histological data illustrated that co-infection with strain LEV8 of coxsackievirus A7 decreased the level of pathological changes induced by SARS-CoV-2 in the lungs. Research into the chemokine/cytokine profile demonstrated that the studied enteroviruses efficiently triggered this part of the antiviral immune response, which is associated with the significant inhibition of SARS-CoV-2 infection. These results demonstrate that there is significant viral interference between the studied strain LEV-8 of coxsackievirus A7 or enterovirus A71 and SARS-CoV-2 in vitro and in vivo.
Tepilamide Fumarate as a Novel Potentiator of Virus-Based Therapy
Authors: Alwithenani Akram , Arulanandam Rozanne , Wong Boaz , Spinelli Marcus M. , Chen Andrew , Maznyi Glib , Gilchrist Victoria H. , Alain Tommy , Diallo Jean-Simon
Published: June 2024

Oncolytic virotherapy, using viruses such as vesicular stomatitis virus (VSVΔ51) and Herpes Simplex Virus-1 (HSV-1) to selectively attack cancer cells, faces challenges such as cellular resistance mediated by the interferon (IFN) response. Dimethyl fumarate (DMF) is used in the treatment of multiple sclerosis and psoriasis and is recognized for its anti-cancer properties and has been shown to enhance both VSVΔ51 and HSV-1 oncolytic activity. Tepilamide fumarate (TPF) is a DMF analog currently undergoing clinical trials for the treatment of moderate-to-severe plaque psoriasis. The aim of this study was to evaluate the potential of TPF in enhancing the effectiveness of oncolytic viruses. In vitro, TPF treatment rendered 786-0 carcinoma cells more susceptible to VSVΔ51 infection, leading to increased viral replication. It outperformed DMF in both increasing viral infection and increasing the killing of these resistant cancer cells and other cancer cell lines tested. Ex vivo studies demonstrated TPF’s selective boosting of oncolytic virus infection in cancer cells without affecting healthy tissues. Effectiveness was notably high in pancreatic and ovarian tumor samples. Our study further indicates that TPF can downregulate the IFN pathway through a similar mechanism to DMF, making resistant cancer cells more vulnerable to viral infection. Furthermore, TPF’s impact on gene therapy was assessed, revealing its ability to enhance the transduction efficiency of vectors such as lentivirus, adenovirus type 5, and adeno-associated virus type 2 across various cell lines. This data underscore TPF’s potential role in not only oncolytic virotherapy but also in the broader application of gene therapy. Collectively, these findings position TPF as a promising agent in oncolytic virotherapy, warranting further exploration of its therapeutic potential.
HIV-Induced Thymic Insufficiency and Aging-Related Immunosenescence on Immune Reconstitution in ART-Treated Patients
Authors: Guedes Maria Carolina Santos , Carvalho-Silva Wlisses Henrique Veloso , Andrade-Santos José Leandro , Brelaz-de-Castro Maria Carolina Accioly , Souto Fabrício Oliveira , Montenegro Lílian Maria Lapa , Guimarães Rafael Lima
Published: June 2024

The mechanisms underlying unsatisfactory immune reconstitution in HIV-1 positive patients under ART have not been fully elucidated, even after years of investigation. Thus, this study aimed to assess the correlation between age and thymic production profile, and its influence on inadequate immunological recovery. Here, 44 ART-treated patients with undetectable plasma HIV-1 load (<40 copies/mL) were classified as 31 immunological responders (IR) and 13 immunological non-responders (INR), according to their CD4+ T-cell count after 18 months of ART. The thymic function was assessed by identifying recent thymic emigrants (RTEs) CD4+ T cells (CD4+/CD45RA+CD31+) in PBMCs using flow cytometry. Clinical data were also analyzed from medical records. The INR group showed a higher age at ART initiation (41 ± 3.0) compared to the IR (33.7 ± 2.1) group (p = 0.041). Evaluating RTE CD4+ T-cells, we observed a lower percentage in the INR group (19.5 ± 6.3) compared to the IR group (29.9 ± 11.5) (p = 0.012). There was a strong negative correlation between age at ART initiation and RTE CD4+ T-cells in INRs (r = −0.784, p = 0.004). Our study has highlighted the thymic insufficiency and aging-related immunosenescence with unsatisfactory immunological recovery during ART in HIV-1 positive patients.
Anthrax Vaccination, Gulf War Illness, and Human Leukocyte Antigen (HLA)
Authors: James Lisa M. , Carpenter Adam F. , Engdahl Brian E. , Johnson Rachel A. , Lewis Scott M. , Georgopoulos Apostolos P.
Published: June 2024

We report on a highly significant, positive association between anthrax vaccination and occurrence of Gulf War Illness (GWI) in 111 Gulf War veterans (42 with GWI and 69 controls). GWI was diagnosed in 47.1% of vaccinated veterans but only in 17.2% of non-vaccinated veterans (Pearson χ2 = 7.08, p = 0.008; odds ratio = 3.947; relative risk = 2.617), with 1.6x higher GWI symptom severity in vaccinated veterans (p = 0.007, F-test in analysis of covariance). Next, we tested the hypothesis that the susceptibility to GWI following anthrax vaccination could be due to inability to make antibodies against the anthrax protective antigen (PA), the key protein contained in the vaccine. Since the first step in initiating antibody production would be the binding of PA peptide fragments (typically 15-amino acid long [15-mer]) to peptide-binding motifs of human leukocyte antigen (HLA) Class II molecules, we assessed the binding-motif affinities of such HLA specific molecules to all linear 15-mer peptide fragments of the anthrax PA. We identified a total of 58 HLA Class II alleles carried by the veterans in our sample and found that, of those, 18 (31%) were present in the vaccinated group that did not develop GWI but were absent from the vaccinated group who developed GWI. Remarkably, in silico analyses revealed very high binding affinities of peptide-binding motifs of those 18 HLA alleles with fragments of anthrax vaccine PA, leading to the successful production of anti-PA antibodies. Conversely, the absence of these protective HLA alleles points to a reduced ability to develop antibodies against PA, thus resulting in harmful PA persistence and development of GWI.
Preclinical Immunogenicity and Efficacy Studies for Therapeutic Vaccines for Human Papillomavirus-Type-16-Associated Cancer
Authors: Mohammadi Mohsen , Saha Amara , Giles-Davis Wynetta , Xiang Zhiquan , Novikov Mikhail , Hasanpourghadi Mohadeseh , C. J. Ertl Hildegund
Published: June 2024

The objective of this study was to conduct preclinical immunogenicity and efficacy studies with several therapeutic vaccines for human papillomavirus (HPV)-16-associated cancers expressing the early antigens E5, E6, and E7 with or without E2. The viral oncoproteins were either expressed by themselves as fusion proteins or the fusion proteins were inserted genetically into herpes simplex virus (HSV)-1 glycoprotein D (gD) which, upon binding to the herpes virus entry mediator (HVEM), inhibits an early T cell checkpoint mediated by the B and T cell mediator (BTLA). This, in turn, lowers the threshold for T cell activation and augments and broadens CD8+ T cell responses to the antigens. The fusion antigens were expressed by chimpanzee adenovirus (AdC) vectors. Expression of the HPV antigens within gD was essential for vaccine immunogenicity and efficacy against challenge with TC-1 cells, which express E7 and E6 of HPV-16 but neither E5 nor E2. Unexpectedly, inclusion of E2 increased both CD8+ T cell responses to the other oncoproteins of HPV-16 and the effectiveness of the vaccines to cause the regression of sizable TC-1 tumors.
Enhanced Glycosylation Caused by Overexpression of Rv1002c in a Recombinant BCG Promotes Immune Response and Protects against Mycobacterium tuberculosis Infection
Authors: Weng Shufeng , Li Qingchun , Zhang Tianran , Lin Taiyue , He Yumo , Yang Guang , Wang Honghai , Xu Ying
Published: June 2024

Tuberculosis (TB) is a major global health threat despite its virtual elimination in developed countries. Issues such as drug accessibility, emergence of multidrug-resistant strains, and limitations of the current BCG vaccine highlight the urgent need for more effective TB control measures. This study constructed BCG strains overexpressing Rv1002c and found that the rBCG-Rv1002c strain secreted more glycosylated proteins, significantly enhancing macrophage activation and immune protection against Mycobacterium tuberculosis (M. tb). These results indicate that Rv1002c overexpression promotes elevated levels of O-glycosylation in BCG bacteriophages, enhancing their phagocytic and antigenic presentation functions. Moreover, rBCG-Rv1002c significantly upregulated immune regulatory molecules on the macrophage surface, activated the NF-κB pathway, and facilitated the release of large amounts of NO and H2O2, thereby enhancing bacterial control. In mice, rBCG-Rv1002c immunization induced greater innate and adaptive immune responses, including increased production of multifunctional and long-term memory T cells. Furthermore, rBCG-Rv1002c-immunized mice exhibited reduced lung bacterial load and histological damage upon M. tb infection. This result shows that it has the potential to be an excellent candidate for a preventive vaccine against TB.
Enhanced Timeliness and Co-Administration of Meningitis B Vaccination in Children: Impact of Funding in Valencian Community, Spain
Authors: Juaneda Juan , Estrella-Porter Pablo , Blanco-Calvo Carolina , Orrico-Sánchez Alejandro , Lluch-Rodrigo José Antonio , Pastor-Villalba Eliseo
Published: June 2024

Public funding of vaccines may enhance vaccination rates, co-administration, and timeliness. The impacts of including the serogroup B meningococcus vaccine (MenB) into the national immunisation schedule on vaccination rates, co-administration rates, and timeliness were assessed using a population-based pre-funding (2022) and post-funding (2023) study design. MenB vaccination rates improved after funding and were in line with previously funded vaccines. Co-administration rates also increased significantly. Timely administration increased, protecting children at an early age. Public funding has a positive impact on vaccine accessibility and early protection. Consistent population characteristics highlight the role of funding.
Exploring the Impact of mRNA Modifications on Translation Efficiency and Immune Tolerance to Self-Antigens
Authors: Sioud Mouldy , Juzeniene Asta , Sæbøe-Larssen Stein
Published: June 2024

Therapeutic modified mRNAs are being developed for a broad range of human diseases. However, the impact of potential miscoding of modified mRNAs on self-tolerance remains unknown. Additionally, more studies are needed to explore the effects of nucleoside alkylation on translation. While all six tested modifications are tolerated as substrates by T7 RNA polymerase and inhibited mRNA immunogenicity, the translation efficiency varied significantly depending on the type of modification. In contrast to methylation, ethylation at the N1 position of pseudouridine (Ψ) hindered translation, suggesting that the C5-C1’ glycosidic bond alone is not a critical element for high translation. Inhibition of mRNA translation was also observed with 5-methoxyuridine modification. However, this inhibition was partially alleviated through the optimization of mRNA coding sequences. BALB/c mice immunized with syngeneic ψ-modified mRNA encoding for Wilms’ tumor antigen-1 (WT1) developed a low but significant level of anti-WT1 IgG antibodies compared to those immunized with either unmodified or N1-methyl ψ-modified mRNA. Overall, the data indicate that adding a simple ethyl group (-CH2CH3) at the N1 position of ψ has a major negative effect on translation despite its reduced immunogenicity. Additionally, mRNA containing Ψ may alter translation fidelity at certain codons, which could lead to a breakdown of immune tolerance to self-antigens. This concern should be taken into account during gene replacement therapies, although it could benefit mRNA-based vaccines by generating a diverse repertoire of antigens.
Of Money and Men: A Scoping Review to Map Gender Barriers to Immunization Coverage in Low- and Middle-Income Countries
Authors: Kalbarczyk Anna , Brownlee Natasha , Katz Elizabeth
Published: June 2024

Among the multiple factors impeding equitable childhood immunization coverage in low- and middle-income countries (LMICs), gender barriers stand out as perhaps the most universal. Despite increasing recognition of the importance of gender considerations in immunization programming, there has not yet been a systematic assessment of the evidence on gender barriers to immunization. We conducted a scoping review to fill that gap, identifying 92 articles that described gender barriers to immunization. Studies documented a range of gender influencers across 43 countries in Africa and South Asia. The barrier to immunization coverage most frequently cited in the literature is women’s lack of autonomous decision-making. Access to immunization is significantly impacted by women’s time poverty; direct costs are also a barrier, particularly when female caregivers rely on family members to cover costs. Challenges with clinic readiness compound female caregiver’s time constraints. Some of the most important gender barriers lie outside of the usual purview of immunization programming but other barriers can be addressed with adaptations to vaccination programming. We can only know how important these barriers are with more research that measures the impact of programming on gender barriers to immunization coverage.
The Human Genetic Differences in the Outcomes of mRNA Vaccination against COVID-19: A Prospective Cohort Study
Authors: Ryu Ha-Eun , Yoon Jihyun , Choi Ja-Eun , Heo Seok-Jae , Hong Kyung-Won , Jung Dong-Hyuk
Published: June 2024

Background: This study aimed to explore how genetic variations in individuals impact neutralization activity post-mRNA vaccination, recognizing the critical role vaccination plays in curbing COVID-19 spread and the necessity of ensuring vaccine efficacy amidst genetic diversity. Methods: In a 4-week clinical pilot study, 534 healthy subjects received their first COVID vaccine dose, followed by the second dose. Antibody levels were evaluated thrice. From this pool, 120 participants were selected and divided into high- and low-antibody groups based on their levels. Genomic DNA was isolated from peripheral blood mononuclear cells for pilot genome-wide association studies (GWAS) conducted on a single platform. Real-time PCR was used to confirm differences in gene expression identified via GWAS analysis. Results: Three SNPs exceeded the level of p < 1.0 × 10−3. The rs7795433 SNP of the HDAC9 gene (7q21.1) showed the strongest association with COVID-19 vaccination under the additive model (OR = 5.63; p = 3 × 10−5). In the PCR experiments, the AA genotype group showed that the gene expression level of HDAC9 was likely to be decreased in the low-antibody-formation group at the time of vaccination. Conclusion: We found that AA genotype holders (rs7795433 SNP of the HDAC9 gene) have a high probability of having a higher antibody count when vaccinated, and GG type holders have a high probability of the opposite. These findings show that the genetic characteristics of vaccinated people may affect antibody production after COVID vaccination.
Factors Influencing COVID-19 Vaccine Confidence and Uptake in Australian Adults
Authors: Williams Charles Travers , Saini Bandana , Zaidi Syed Tabish R. , Kali Christina , Moujalli Grace , Castelino Ronald
Published: June 2024

In January 2021, Australia initiated a national COVID-19 vaccine rollout strategy but faced setbacks, leading to negative press and media controversy, which may have diminished vaccine confidence. This study aimed to assess the factors influencing vaccine confidence in Australian adults (≥18 years of age) following the administration of a COVID-19 vaccine. Conducted at Blacktown Hospital, Sydney, a cross-sectional survey with 1053 respondents gauged vaccine confidence and influencing factors. The results showed overall high confidence (mean score 33/40). Trusted sources included the Australian Department of Health (77.8%), NSW Health (76.7%), and general practitioners (53.7%), while social media was distrusted (5.9%). The motivations for vaccination varied: university-educated individuals prioritised personal health (X2 = 17.81; p < 0.001), while religious and/or older respondents (≥50 years of age) emphasised community (X2 = 11.69; p < 0.001) and family protection (X2 = 17.314; p < 0.001). Multivariate logistic regression revealed use of the Australian Department of Health website as a trusted source of COVID-19 information as the strongest predictor of high confidence (>30; OR 1.43; p = 0.041), while exposure to fake news decreased confidence (OR 0.71; p = 0.025). The study underscores the importance of reliable health information sources in bolstering vaccine confidence and highlights the detrimental effects of misinformation. Promoting awareness of trustworthy health channels is crucial to combat vaccine hesitancy in Australia.
COVID-19 Vaccine Uptake and Effectiveness by Time since Vaccination in the Western Cape Province, South Africa: An Observational Cohort Study during 2020–2022
Authors: Kassanjee Reshma , Davies Mary-Ann , Heekes Alexa , Mahomed Hassan , Hawkridge Anthony , Morden Erna , Jacobs Theuns , Cohen Cheryl , Moultrie Harry , Lessells Richard , Van Der Walt Nicolette , Arendse Juanita , Wolter Nicole , Walaza Sibongile , Jassat Waasila , von Gottberg Anne , Hannan Patrick , Feikin Daniel , Cloete Keith , Boulle Andrew
Published: June 2024

There are few data on the real-world effectiveness of COVID-19 vaccines and boosting in Africa, which experienced widespread SARS-CoV-2 infection before vaccine availability. We assessed the association between vaccination and severe COVID-19 in the Western Cape, South Africa, in an observational cohort study of >2 million adults during 2020–2022. We described SARS-CoV-2 testing, COVID-19 outcomes, and vaccine uptake over time. We used multivariable cox models to estimate the association of BNT162b2 and Ad26.COV2.S vaccination with COVID-19-related hospitalization and death, adjusting for demographic characteristics, underlying health conditions, socioeconomic status proxies, and healthcare utilization. We found that by the end of 2022, 41% of surviving adults had completed vaccination and 8% had received a booster dose. Recent vaccination was associated with notable reductions in severe COVID-19 during periods dominated by Delta, and Omicron BA.1/2 and BA.4/5 (sub)lineages. During the latest Omicron BA.4/5 wave, within 3 months of vaccination or boosting, BNT162b2 and Ad26.COV2.S were each 84% effective against death (95% CIs: 57–94 and 49–95, respectively). However, distinct reductions of effectiveness occurred at longer times post completing or boosting vaccination. Results highlight the importance of continued emphasis on COVID-19 vaccination and boosting for those at high risk of severe COVID-19, even in settings with widespread infection-induced immunity.
Hematological and Biochemical Effects Associated with Prolonged Administration of the NSAID Firocoxib in Adult Healthy Horses
Authors: Ignácio Fernanda Saules , Garcia Luana Venâncio , de Souza Giovanna Gati , Amatti Lidiana Zanetti , de Barros Luiz Daniel , Bergfelt Don R. , Camargo Giovana Siqueira , de Meira Cezinande , de Almeida Breno Fernando Martins
Published: June 2024

Non-steroidal anti-inflammatory drugs (NSAIDs) represent one of the most commonly used classes of drugs in both human and veterinary medicine. However, many clinical side effects have been observed, especially when treatment has been prolonged. While the anti-inflammatory efficacy and safety of repeated administration of firocoxib (Previcox®), which is a selective NSAID COX-2 inhibitor, has been evaluated for short-term use (one to fourteen days), its clinical relevance for longer-term use is not known. As a preliminary study, healthy, adult male and female horses (n = 7) were treated with firocoxib for 40 days concomitant with the collection of blood samples encompassing treatment to assess hematological and biochemical endpoints. Daily oral administration of firocoxib was performed with one 57 mg tablet/animal (0.11–0.14 mg/kg), which was crushed and mixed with feed. Blood samples were collected one day before treatment (D0 or basal sample), during (D10, D20, D30, and D40), and after treatment (D55 and D70). Results indicated some hematological and biochemical effects were significantly reduced (p < 0.05) towards the end of treatment on D40 relative to pre-treatment or baseline values on D0. Post-treatment, all values returned to pre-treatment values within 30 days without any apparent clinical adversities. In conclusion, while these preliminary results are favorable for prolonged use of firocoxib in horses, future studies are required to evaluate the efficacy of prolonged use accompanied with other clinically relevant endpoints in healthy as well as injured or diseased animals.
Application of the Data-Driven Method and Hydrochemistry Analysis to Predict Groundwater Level Change Induced by the Mining Activities: A Case Study of Yili Coalfield in Xinjiang, Norwest China
Authors: Luo Ankun , Dong Shuning , Wang Hao , Cao Haidong , Wang Tiantian , Hu Xiaoyu , Wang Chenyu , Zhang Shouchuan , Qu Shen
Published: June 2024

As the medium of geological information, groundwater provides an indirect method to solve the secondary disasters of mining activities. Identifying the groundwater regime of overburden aquifers induced by the mining disturbance is significant in mining safety and geological environment protection. This study proposes the novel data-driven algorithm based on the combination of machine learning methods and hydrochemical analyses to predict anomalous changes in groundwater levels within the mine and its neighboring areas induced after mining activities accurately. The hydrochemistry analysis reveals that the dissolution of carbonate and evaporite and the cation exchange function are the main hydrochemical process for controlling the groundwater environment. The anomalous change in the hydrochemistry characteristic in different aquifers reveals that the hydraulic connection between different aquifers is enhanced by mining activities. The continuous wavelet coherence is used to reveal the nonlinear relationship between the groundwater level change and external influencing factors. Based on the above analysis, the groundwater level, precipitation, mine water inflow, and unit goal area could be considered as the input variables of the hydrological model. Two different data-driven algorithms, the Decision Tree and the Long Short-Term Memory (LSTM) neural network, are introduced to construct the hydrological prediction model. Four error metrics (MAPE, RMSE, NSE and R2) are applied for evaluating the performance of hydrological model. For the NSE value, the predictive accuracy of the hydrological model constructed using LSTM is 8% higher than that of Decision Tree algorithm. Accurately predicting the anomalous change in groundwater level caused by the mining activities could ensure the safety of coal mining and prevent the secondary disaster of mining activities.
Application of a Bayesian-Based Integrated Approach for Groundwater Contamination Sources Parameter Identification Considering Observation Error
Authors: Yan Xueman , An Yongkai
Published: June 2024

Groundwater contamination source (GCS) parameter identification can help with controlling groundwater contamination. It is proverbial that groundwater contamination concentration observation errors have a significant impact on identification results, but few studies have adequately quantified the specific impact of the errors in contamination concentration observations on identification results. For this reason, this study developed a Bayesian-based integrated approach, which integrated Markov chain Monte Carlo (MCMC), relative entropy (RE), Multi-Layer Perceptron (MLP), and the surrogate model, to identify the unknown GCS parameters while quantifying the specific impact of the observation errors on identification results. Firstly, different contamination concentration observation error situations were set for subsequent research. Then, the Bayesian inversion approach based on MCMC was used for GCS parameter identification for different error situations. Finally, RE was applied to quantify the differences in the identification results of each GCS parameter under different error situations. Meanwhile, MLP was utilized to build a surrogate model to replace the original groundwater numerical simulation model in the GCS parameter identification processes of these error situations, which was to reduce the computational time and load. The developed approach was applied to two hypothetical numerical case studies involving homogeneous and heterogeneous cases. The results showed that RE could effectively quantify the differences caused by contamination concentration observation errors, and the changing trends of the RE values for GCS parameters were directly related to their sensitivity. The established MLP surrogate model could significantly reduce the computational load and time for GCS parameter identification. Overall, this study highlights that the developed approach represents a promising solution for GCS parameter identification considering observation errors.
A Mini-Review on Safe Treatment and Valorization of Salt Waste in Chemical Production Processes in China
Authors: Lv Yang , Wang Yi , Zhang Dapeng , Wu Chaoyue , Zhang Jun , Zhao Zehua , Nabi Mohammad , Luo Xuan , Xiao Keke
Published: June 2024

In chemical production processes in China, a huge volume of salt waste is produced, contributing to environmental pollution due to the lack of proper treatment procedures to manage this waste effectively. With the current lack of proper treatment methods for salt waste disposal, landfill emerges as the predominant disposal approach adopted in China, exacerbating environmental concerns associated with the accumulation of such waste. In addition, this method fails to reuse and recycle salt waste. This review paper presents pertinent research on the safe disposal and utilization of salt waste in China. Well-known valorization methods, such as oxidation, thermal treatment, washing separation, precipitation, and evaporation crystallization, are comprehensively reviewed. The current technologies for recovering resources from salt waste and recommendations for its treatment and valorization are analyzed. This research work offers a reference for future resource utilization of industrial salt waste.