bluuebunny/crossref_metadata_2025 huggingface dataset:
Невербальна інформація в криміналістиці: доктринальні засади
Authors: ВАЩУК ОЛЕСЯ
Published: December 2021

Метою статті є розроблення доктринальних засад використання невербальної інформації в криміналістиці. Актуальність такого дослідження полягає в тому, що вже розроблені положення щодо використання невербальної інформації в криміналістиці є достатніми для подальшого розроблення їх доктринальних засад, теоретично обґрунтовуючи таку інтегративну можливість, підвищуючи їхню роль та значення на практиці. Наголошено, що розроблення доктринальних засад використання невербальної інформації має такі теоретичні напрями, як то визначення і характеристика засад невербальної інформації в криміналістиці (теорія) та формування наукових положень щодо сприйняття і використання невербальної інформації у кримінальному провадженні.
Здійснено узагальнення сучасного криміналістичного знання про положення невербальної інформації в кримінальному провадженні. Виділено етапи формування наукових підходів до розробленості проблеми, пов’язаної з дослідженням невербальної інформації в криміналістиці із трьох напрямів: психологічного, кримінального процесуального та криміналістичного. Підкреслено, що розробленими та вживаними є такі два види невербальної інформації в криміналістиці: невербальна інформація, що наявна в речах матеріальної обстановки (навколишнього середовища, енвайроментджерельна); антроподжерельна невербальна інформація, що надходить із зовнішності учасника кримінально-релевантної події (у статиці) й антроподжерельні функціональні прояви почуттів, думок у вигляді міміки, жестів, рухів тіла, особливостей мовлення, погляду, що виражаються і сприймаються у процесі комунікативного процесу (в динаміці).
Визначено принципи використання невербальної інформації в криміналістиці: ретроспективний підхід до вивчення невербальних проявів; єдність діяльності і свідомості особи; урахування взаємозв’язку фізичних і психічних проявів при дешифруванні невербальної комунікації; врахування двоаспектності проявів вербальної та невербальної поведінки.
Сформовано доктринальні засади використання невербальної інформації в кримінальному провадженні. Розкрито значення доктринального напрацювання використання невербальної інформації в криміналістиці на теоретичному та практичному рівнях. Визначено, що метою такого доктринального підходу є інтенси-фікація використання положень невербальної інформації в криміналістиці, що сприяє вдосконаленню практики боротьби зі злочинністю. Зроблено висновок, що предметом наукового пізнання цих засад є енвайроментджерельна невербальна інформація та прояви антроподжерельної невербальної інформації осіб, причетних до кримінально-релевантної події на рівні злочинної діяльності і діяльності у сфері кримінального провадження. Запроновано впровадження у доктринальний обіг нових наукових категорій (“антроподжерельна невербальна інформація”, “енвайроментджерельна невербальна інформація”, “верифікація”, “валідація”, “вербалізація”).
FAKTOR-FAKTOR YANG BERPENGARUH TERHADAP KEPATUHAN WAJIB PAJAK PASCA PENERAPAN PERATURAN PEMERINTAH NOMOR 23 TAHUN 2018
Authors: Santuo Santuo
Published: December 2021

Kepatuhan wajib pajak merupakan salah satu faktor penentu dalam pencapaian penerimaan pajak.Pemerintah telah melakukan berbagai upaya untuk meningkatkan kepatuhan wajib pajak. Salahsatunya adalah dengan menerbitkan Peraturan Pemerintah Nomor 23 Tahun 2018. Penelitian inibertujuan untuk mengetahui pengaruh perubahan tarif pajak, penerapan asas keadilan perpajakan danpelaksanaan sosialisasi perpajakan terhadap kepatuhan wajib pajak pada Kantor Pelayanan PajakPratama Bulukumba setelah penerapan Peraturan Pemerintah Nomor 23 Tahun 2018. Jumlah sampeldalam penelitian ini sebanyak 120 wajib pajak yang terdaftar pada Kantor Pelayanan Pajak PratamaBulukumba, yang ditentukan dengan teknik cluster random sampling yaitu dengan mengambilresponden secara acak dari tiga kabupaten yang ada di wilayah kerja Kantor Pelayanan Pajak PratamaBulukumba. Penelitian ini menggunakan kuesioner sebagai instrumen pengumpulan data. Analisisdata menggunakan analisis regresi linear berganda dengan uji hipotesis parsial dan simultan. Hasilpenelitian menunjukkan bahwa tarif pajak, asas keadilan perpajakan dan sosialisasi perpajakanberpengaruh secara positif dan signifikan terhadap kepatuhan wajib pajak. Hal ini berarti bahwakepatuhan wajib pajak pada Kantor Pelayanan Pajak Pratama Bulukumba setelah penerapan PeraturanPemerintah Nomor 23 Tahun 2018 secara mayoritas dipengaruhi oleh perubahan tarif pajak,penerapan asas keadilan perpajakan dan pelaksanaan sosialisasi perpajakan atas penerapan peraturantersebut serta selebihnya dipengaruhi oleh faktor lain yang tidak dimasukkan dalam model penelitianini.
EVALUASI PELAKSANAAN E-TENDERING PADA UNIT LAYANAN PENGADAAN DAERAH KEMENTERIAN KEUANGAN PROVINSI SULAWESI SELATAN
Authors: Salupra Yohanis
Published: December 2021

Pelaksanaan pengadaan barang/jasa pemerintah melalui e-tendering di Indonesia masih mengalami beberapa masalah. Seperti halnya pelaksanaan e-tendering pada Unit Layanan Pengadaan Daerah Kementerian Keuangan Provinsi Sulawesi Selatan yang mengalami tender gagal. Pada tahun 2016, jumlah tender gagal sebanyak enam paket tender gagal sebesar Rp4.695.581.000,00. Kemudian  pada tahun 2017, jumlah paket tender gagal sebanyak empat paket sebesar Rp36.531.445.000,00. Pada tahun 2018, sebanyak empat paket mengalami tender gagal senilai Rp23.407.124.000,00. Sampai Agustus 2019, tender gagal terjadi sebanyak dua paket dengan nilai Rp2.061.932.000,00. Selain itu, masih terdapat adanya persekongkolan penyedia dalam mengikuti tender. Penelitian ini bertujuan untuk melakukan evaluasi terhadap Pelaksanaan E-Tendering Pada Unit Layanan Pengadaan Daerah Kementerian Keuangan Provinsi Sulawesi Selatan dengan menggunakan pendekatan deskriptif kualitatif melalui wawancara, observasi, dan studi dokumentasi. Hasil penelitian menunjukkan bahwa terdapat keterlambatan penyelesaian tender, dan penerapan reverse auction yang belum sesuai dengan ketentuan. Kecukupan biaya dan hasil dalam pelaksanaan e-tendering telah terpenuhi dan juga terjadi perataan distribusi kegiatan dan manfaat bagi setiap pihak yang terlibat secara langsung dalam proses e-tendering. Kepuasan para pihak dalam melaksanakan e-tendering adalah kemudahan akses sistem, persaingan dan prosesnya yang terbuka, dan mampu mengurangi beban kerja. Maintenance jaringan yang mengganggu jadwal tender, kewajaran nilai honorarium Pokja ULPD, dan proses pemberian penjelasan belum sesuai dengan ketentuan. Pelaksanaan e-tendering pada Unit Layanan Pengadaan Daerah Kementerian Keuangan Provinsi Sulawesi Selatan telah dilaksanakan cukup baik tetapi masih terdapat beberapa hal yang perlu dibenahi, diantaranya status ad hoc Pokja ULPD, kompetensi para pihak, koordinasi antara PPK dengan Pokja ULPD, maintenance jaringan, kewajaran nilai honorarium Pokja ULPD, dan pelaksanaan tahapan pemberian penjelasan.
Disentanglement network data to characterize leatherback sea turtle Dermochelys coriacea bycatch in fixed-gear fisheries
Authors: Dodge KL , Landry S , Lynch B , Innis CJ , Sampson K , Sandilands D , Sharp B
Published: December 2021

To characterize sea turtle bycatch in fixed-gear fisheries in Massachusetts, USA, we analyzed a 15 yr dataset of entanglement reports and detailed documentation from disentanglement operations. Almost all (272) of the 280 confirmed entanglements involved leatherback turtles Dermochelys coriacea. The majority of turtles were entangled in actively fished (96%), commercial (94%) pot/trap gear with unbroken/untriggered weak links, specifically the buoy lines marking lobster, whelk, and fish traps. Most reports came from recreational boaters (62%) and other sources (26%), rather than commercial fishers (12%). Leatherback entanglements occurred from May to November, with peak reporting in August, and included adult males, adult females, and subadults. All entanglements involved the turtle’s neck and/or front flippers, with varying degrees of visible injuries; 47 entangled leatherbacks were dead in gear, 224 were alive at first sighting, and 1 case was unknown. Post-release monitoring suggested turtles can survive for days to years after disentanglement, but data were limited. While the observed entanglements in our study are low relative to global bycatch, these numbers should be considered a minimum. Our findings are comparable to observed numbers of leatherbacks taken in Canadian fixed-gear fisheries, and represent just one of multiple, cumulative threats in the North Atlantic. Managers should focus on strategies to reduce the co-occurrence of sea turtles and fixed-fishing gear, including reductions in the number of buoy lines allowed (e.g. replace single sets with trawls), seasonal and area closures targeted to reduce sea turtle-gear interaction, and encourage the development of emerging technologies such as ‘ropeless’ fishing.
Acute and Chronic Effects of High-Intensity Interval Training (HIIT) on Postexercise Intramuscular Lipid Metabolism in Rats
Authors: CHEN M , ZHOU L , CHEN S , SHANGGUAN R , QU Y , SUN J
Published: December 2021

Recovery from exercise refers to the period between the end of a bout of exercise and the subsequent return to a resting or recovered state. It is a dynamic period in which many physiological changes occur. A large amount of research has evaluated the effect of training on intramuscular lipid metabolism. However, data are limited regarding intramuscular lipid metabolism during the recovery period. In this study, lipid metabolism-related proteins were examined after a single bout of exercise in a time-dependent way to explore the mechanism of how exercise induces intramuscular lipid metabolism adaptation. Firstly, all rats in the exercise group underwent a five-week training protocol (HIIT, five times/week), and then performed a more intense HIIT session after 72 h of the last-time five-week training. After that, rats were sampled in a time-dependent way, including 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, and 96 h following the acute training session. Our results discovered that five weeks of HIIT increased the content of intramuscular triglyceride (IMTG) and enhanced the lipolytic and lipogenesis-related proteins in skeletal muscle. Furthermore, IMTG content decreased immediately post HIIT and gradually increased to baseline levels 48 h postexercise, continuing to over-recover up to 96 h postexercise. Following acute exercise, lipolytic-related proteins showed an initial increase (6-12 h) before decreasing during recovery. Conversely, lipogenesis-related proteins decreased following exercise (6-12 h), then increased in the recovery period. Based on the changes, we speculate that skeletal muscle is predominated by lipid oxidative at the first 12 h postexercise. After this period, lipid synthesis-related proteins increased, which may be the result of body recovery. Together, these results may provide insight into how the lipid metabolism-related signaling changes after chronic and acute HIIT and how protein levels lipid metabolism correlates to IMTG recovery.
Initial Anticoagulant Management of Deep Vein Thrombosis/Venous Thromboembolism in Primary Care: Review of Current Approaches
Authors: Martin Pablo Millares
Published: December 2021

Background and Aims: The initial management of deep vein thrombosis is starting to happen in general practice. New treatments are available to allow this shift, but guidance is variable. The aim of this study was to understand current choices used in general practice in the UK and to determine if there is a more efficient treatment, considering variability observed locally. Methods: A systematic literature review and freedom of information requests to England’s 198 clinical commissioning groups (CCG) were used to gather information on treatment options and current uses, respectively. Over 100 papers were assessed, and information from 177 CCG was obtained. Results: There is noninferiority between injectable low-molecular-weight heparin and novel oral anticoagulant treatments. Fifteen CCG offered variable, but also limited, options of treatment. Patient choice was not necessarily considered. Conclusion: There is variability in England on availability and choice of therapy for the initial management of deep vein thrombosis at present, which may also be the case elsewhere. The implementation of evidence-based guidelines should be carefully considered in all settings and countries.
A Virtual Live Case of Bi/Trifurcation Percutaneous Coronary Intervention of the
Left Main
Authors: Rodgers Louise
Published: December 2021

On Day 2 of EuroPCR 2021, discussants from around the world took to their screens to join Jean Fajadet, PCR Vice-Chairman, and his panel, live from their studio in Paris, France. The aim was to understand how to adapt treatment strategy to the underlying anatomy in a case of left main bifurcation percutaneous coronary intervention.
Breast Cancer Immunotherapy: From Biology to Current Clinical Applications
Authors: Leal Jorge Henrique Santos , McArthur Heather
Published: December 2021

Therapeutic strategies for the treatment of breast cancer have historically been determined by the presence or absence of hormone receptors and HER2 amplification and/or protein expression. For patients with breast cancer that lack these biomarkers, the so-called ‘triple-negative’ subtype, chemotherapy has been the cornerstone of cure and palliation. However, with the recent successful development of immune checkpoint molecules that target cytotoxic T-lymphocyte antigen-4, programmed cell death-1 (PD-1), and PD-ligand 1 (PD-L1), improved survival has been reported across a range of tumour types including melanoma, lung, and bladder cancer. In metastatic breast cancer, trials of single-agent immune checkpoint inhibitors (ICI) have resulted in limited overall response rates; however, strategies that combine local or systemic therapies with ICI have improved response rates and, in some cases, improved survival. For example, the addition of an anti-PD-L1 inhibitor, atezolizumab, to nab-paclitaxel chemotherapy for newly diagnosed metastatic triple-negative breast cancer demonstrated an improvement in overall survival in an informal analysis of the PD-L1-positive subset in a recently reported Phase III clinical trial. These results ultimately led to U.S. Food and Drug Administration (FDA) approval for an ICI for the treatment of breast cancer, with numerous other health authorities following suit. Herein, the authors describe the biology behind ICI, the rationale for ICI administration in breast cancer, the related clinical trial data reported to date, and promising future strategies.
When Regenerative Medicine Faces the Challenges of Reproductive Medicine: A Review Study on Recent Advances in the Strategies for Derivation of Gametes from Stem Cells
Authors: Juliá María Gil , Medrano José V.
Published: December 2021

The murine model has allowed for the replication of all developmental stages of the mammalian germline in vitro, from embryonic stem cells to epiblast cells, primordial germ cells, and finally into functional haploid gametes. However, because of interspecies differences between mice and humans, these results are yet to be replicated in our species. Reports on the use of stem cells as a source of gametes, retrieved from public scientific databases, were analysed and classified according to the animal model used, the stem cell source and type, the differentiation strategy, and its potential application. This review offers a comprehensive compilation of recent publications of key events in the derivation of germ cells and gametogenesis in vitro, in both mice and human models. Additionally, studies intending to replicate the different stages in human cells in vitro, in order to obtain cells with a phenotype akin to functional human gametes, are also depicted. The authors present options for deriving gametes from stem cells in vitro and different reproductive options for specific groups of patients. Lastly, the potential applications of in vitro human gametogenesis are evaluated as well as the main limitations of the techniques employed. Even though it appears that we are far from being able to obtain gametes from pluripotent stem cells in vitro as a viable reproductive option, its current academic and clinical implications are extremely promising.
Non-papillary Percutaneous Puncture: A Safe Approach to Consider
Authors: Tsaturyan Arman , Kallidonis Panagiotis , Liatsikos Evangelos
Published: December 2021

Percutaneous nephrolithotomy (PCNL) is the ‘gold standard’ treatment modality for renal stones larger than 2 cm. It can be also applied to manage smaller renal stones and large, impacted stones located in the proximal ureter. Nevertheless, even in experienced hands, the PCNL procedure is associated with the development of several complications. Despite the existing extensive research in this field, studies evaluating the effect of puncture site on perioperative bleeding are very few. In part, this can be explained by the dogma that the safest way to perform kidney puncture is through the renal papilla. In this paper, the authors summarise their experience of non-papillary PCNL and demonstrate this puncture technique for PCNL tract establishment.
CNN for Image Identification of Hiragana Based on Pattern Recognition using CNN
Authors: Umam Chaerul , Krismawan Andi Danang , Ali Rabei Raad
Published: December 2021

Hiragana is one of the letters in Japanese. In this study, CNN (Convolutional Neural Network) method used as identication method, while he preprocessing used thresholding. Then carry out the normalization stage and the filtering stage to remove noise in the image. At the training stage use maxpooling and danse methods as a liaison in the training process, wherea in testing stage using the Adam Optimizer method. Here, we use 1000 images from 50 hiragana characters with a ratio of 950: 50, 950 as training data and 50 data as testing data. Our experiment yield accuracy in 95%.
Analisis Perubahan Lahan Sawah Areal Pengairan Bendungan Dikaitkan Dengan Ketahanan Pangan
Authors: Nursajidah Nursajidah , Golok Jaya La Ode Muh. , Iradat Salihin Laode Muh.
Published: December 2021

Abstrak: Konversi lahan pertanian menimbulkan dampak negatif terhadap pembangunan pertanian, sehingga menurunkan kapasitas produksi pertanian, rusaknya sistem pengairan dan hilangnya investasi yang telah ditanamkan dalam membangun jaringan irigasi dan pembuatan teras pematang sawah. Penelitian ini bertujuan untuk: (1) mengetahui perubahan lahan sawah menggunakan Citra Landsat multitemporal; (2) mengetahui pengaruh perubahan lahan sawah areal Bendungan Wawotobi terhadap pemenuhan kebutuhan dan ketahanan pangan. Metode analisis data dalam penelitian ini adalah: (1) analisis penggunaan lahan dengan metode berbasis objek; (2) analisis estimasi produksi dan kebutuhan beras. Hasil penelitian ini antara lain: (1) perubahan luas lahan sawah tahun 2009 seluas 4.291,6 ha dan meningkat menjadi seluas 7.162,67 ha pada tahun 2018; (2) analisis estimasi produksi tanaman padi pada empat Kecamatan di Kabupaten Konawe dalam areal Pengairan Bendungan Wawotobi, yaitu Kecamatan Pondidaha didapat  sebesar 8.138,99 ton dan kebutuhan beras 837,9 ton/tahun dengan jumlah penduduk 11.970 jiwa, Kecamatan Wonggeduku sebesar 48.416,13 ton dan kebutuhan beras 952,14 ton/tahun dengan jumlah penduduk 13.602 jiwa, Kecamatan Wawotobi sebesar 9.801,20 ton dan kebutuhan beras 1.619,8 ton/tahun dengan jumlah penduduk 23.140 jiwa, dan Kecamatan Unaaha sebesar 1.984,27 ton dan kebutuhan beras 1.821,89 ton/tahun dengan jumlah penduduk 26.027 jiwa.Kata Kunci: Sawah, Perubahan Lahan Sawah, Ketahanan Pangan
Pengetahuan dan Sikap Ibu Menyusui Tentang Asi Eksklusif Pada Masa Pandemi Covid-19
Authors: Kambera Loli , Pratiwi Bintang Agustina , Yanuarti Riska , Oktarianita Oktarianita , Wati Nopia
Published: December 2021

Penelitian ini bertujuan untuk mengetahui perbedaan pengetahuan dan sikap ibu sebelum dan sesudah diberikan edukasi tentang ASI Eksklusif di masa pandemi Covid-19 Wilayah Kerja Puskesmas Lingkar Timur Kota Bengkulu. Jenis penelitian quassy experimental dengan rancangan one group pretest-posttest design. Penelitian dilakukan di wilayah kerja Puskesmas Lingkar Timur. Populasi penelitian yaitu semua Ibu menyusui yang memiliki bayi usia 0 – 6 bulan di wilayah kerja Puskesmas Lingkar Timur Kota Bengkulu berjumlah sebanyak 59 orang, selanjutnya sampel diambil dengan teknik purposive sampling. Data yang terkumpul dianalisis, univariat dan bivariat (paired sample t test). Hasil analisis univariat menunjukan bahwa rata-rata skor pengetahuan sebelum edukasi sebesar 11,76 dan sesudah edukasi sebesar 15,69, sedangkan rata-rata skor sikap sebelum edukasi sebesar 78,93 dan sesudah edukasi sebesar 98,80. Hasil analisis bivariat menunjukan bahwa terdapat perbedaan signifikan pengetahuan (P value = 0.000) dan sikap (P Value = 0.000) sebelum diberikan edukasi dan sesudah diberikan edukasi ASI Eksklusif. Edukasi sebaiknya terus dilakukan terutama selama masa pandemic, sehingga ibu tetap semangat terus memberikan ASI eksklusif
The Implementasi Kegiatan Imunisasi Posyandu dengan Menggunakan Juknis Imunisasi pada masa Pandemi Covid -19
Authors: Permatasari Rahmi , Liliandriani Andi
Published: December 2021

Penelitian ini bertujuan untuk mengetahui bagaimana implementasi dari kegiatan posyandu khususnya kegiatan imunisasi posyandu dimasa Pandemi Covid-19. Peneliti ini bersifat deskriptif non eksperimental, menggunakan pendekatan kualitatif dengan melakukan survey lapangan serta wawancara mendalam pihak posyandu dan masyarakat yang terlibat dalam kegiatan imunisasi posyandu Puskesmas Matakali. Pengambilan sampel manggunakan teknik purposive sampling hingga informasi yang dibutuhkan terpenuhi dan dilanjutkan dengan triangulasi data dan triangulasi sumber. Triagulasi data dilihat dari buku panduan yang di lihat dari Juknis Imunisasi pada Masa Pandemi Covid 19 yang dikeluarkan oleh Direktorat Surveilans dan Karantina Kesehatan Dirjen Pencegahan dan Pengendalian Penyakit Kemenkes RI tahun 2020.Hasil penelitian menunjukan kegiatan imunisasi posyandu Puskesmas Matakali dimasa pandemic Covid-19 tetap dilaksanakan sesuai dengan prosedur imunisasi masa pandemic seperti tetap menjaga jarak antar peserta, memastikan peserta dan tenaga kesehatan dalam keadaan sehat mengatur jumlah peserta dan mengatur posisi tempat duduk, menyiapkan keperluan cuci tangan dan menghimbau masyarakat membawa kain sendiri untuk penimbangan dacin. Sempat terjadi penundaan kegiatan imunisasi di awal pandemi karena adanya pembatasan kegiatan. Selain itu untuk pasokan hazmat juga terbatas mengalami keterbatasan sehingga APD yang digunakan untuk imunisasi selanjutnya menggunakan APD lain seperti sarung tangan, apron, penutup kepala, dan protokol 3M. Kesimpulan yaitu posyandu Puskesmas Matakali menjalankan kegiatan imunisasi berdasarkan Juknis Imunisasi pada Masa Pandemi Covid 19
The Application of Wearable Glucose Sensors in Point-of-Care Testing
Authors: Zhang Sheng , Zeng Junyan , Wang Chunge , Feng Luying , Song Zening , Zhao Wenjie , Wang Qianqian , Liu Chen
Published: December 2021

Diabetes and its complications have become a worldwide concern that influences human health negatively and even leads to death. The real-time and convenient glucose detection in biofluids is urgently needed. Traditional glucose testing is detecting glucose in blood and is invasive, which cannot be continuous and results in discomfort for the users. Consequently, wearable glucose sensors toward continuous point-of-care glucose testing in biofluids have attracted great attention, and the trend of glucose testing is from invasive to non-invasive. In this review, the wearable point-of-care glucose sensors for the detection of different biofluids including blood, sweat, saliva, tears, and interstitial fluid are discussed, and the future trend of development is prospected.
The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis
Authors: Colin-Pierre Charlie , Berthélémy Nicolas , Belloy Nicolas , Danoux Louis , Bardey Vincent , Rivet Romain , Mine Solène , Jeanmaire Christine , Maquart François-Xavier , Ramont Laurent , Brézillon Stéphane
Published: December 2021

The hair renewal involves changes in the morphology of the hair follicle and its micro-vascularization. In alopecia, the hair cycle is accelerated, resulting in the formation of thinner and shorter hair. In addition, alopecia is associated with a decrease in the micro-vascularization of the hair follicles. In this study, the role of glypicans (GPCs) was analyzed in the regulation of the angiogenesis of human dermal microvascular endothelial cells (HDMEC). The analysis of glypican gene expression showed that GPC1 is the major glypican expressed by human keratinocytes of outer root sheath (KORS), human hair follicle dermal papilla cells (HHFDPC) and HDMEC. KORS were demonstrated to secrete VEGF and HGF. The HDMEC pseudotube formation was induced by KORS conditioned media (KORSCM). It was totally abrogated after GPC1 siRNA transfection of HDMEC. Moreover, when cleaved by phospholipase C (PLC), GPC1 promotes the proliferation of HDMEC. Finally, GPC1 was shown to interact directly with VEGFR2 or c-Met to regulate angiogenesis induced by the activation of these receptors. Altogether, these results showed that GPC1 is a key regulator of microvascular endothelial cell angiogenesis induced by VEGF and HGF secreted by KORS. Thus, GPC1 might constitute an interesting target to tackle alopecia in dermatology research.
Low Dose Chronic Angiotensin II Induces Selective Senescence of Kidney Endothelial Cells
Authors: Khan Irfan , Schmidt Marcel O. , Kallakury Bhaskar , Jain Sidharth , Mehdikhani Shaunt , Levi Moshe , Mendonca Margarida , Welch William , Riegel Anna T. , Wilcox Christopher S. , Wellstein Anton
Published: December 2021

Angiotensin II can cause oxidative stress and increased blood pressure that result in long term cardiovascular pathologies. Here we evaluated the contribution of cellular senescence to the effect of chronic exposure to low dose angiotensin II in a model that mimics long term tissue damage. We utilized the INK-ATTAC (p16Ink4a–Apoptosis Through Targeted Activation of Caspase 8) transgenic mouse model that allows for conditional elimination of p16Ink4a -dependent senescent cells by administration of AP20187. Angiotensin II treatment for 3 weeks induced ATTAC transgene expression in kidneys but not in lung, spleen and brain tissues. In the kidneys increased expression of ATM, p15 and p21 matched with angiotensin II induction of senescence-associated secretory phenotype genes MMP3, FGF2, IGFBP2, and tPA. Senescent cells in the kidneys were identified as endothelial cells by detection of GFP expressed from the ATTAC transgene and increased expression of angiopoietin 2 and von Willebrand Factor, indicative of endothelial cell damage. Furthermore, angiotensin II induced expression of the inflammation-related glycoprotein versican and immune cell recruitment to the kidneys. AP20187-mediated elimination of p16-dependent senescent cells prevented physiologic, cellular and molecular responses to angiotensin II and provides mechanistic evidence of cellular senescence as a driver of angiotensin II effects.
Exosomal circRNAs: Emerging Players in Tumor Metastasis
Authors: Zhou Hao , He Xiaoyun , He Yuxiang , Ou Chunlin , Cao Pengfei
Published: December 2021

Metastasis is an important feature of malignant tumors, and is the primary cause of poor prognosis and treatment failure, in addition to representing a potentially fatal challenge for cancer patients. Exosomes are small extracellular vesicles 30–150 nm in diameter that transmit cargo, such as DNA, RNA, and proteins, as a means of intercellular communication. Exosomes play crucial roles in a range of human diseases, especially malignant tumors. A growing number of studies have verified that circRNAs can be enveloped in exosomes and transferred from secretory cells to recipient cells, thereby regulating tumor progression, especially tumor metastasis. Exosomal circRNAs regulate tumor cell metastasis not only by regulating the signaling pathways, but also by affecting the tumor microenvironment. Moreover, exosomal circRNAs have the potential to serve as valuable diagnostic biomarkers and novel therapeutic targets in cancer patients. In this review, we summarize the mechanism by which exosomal circRNAs modulate metastatic phenomena in various types of tumors, and put forward the prospects of clinical applications of exosomal circRNAs in tumor therapy.
An Apoptosis-Related Gene Prognostic Index for Colon Cancer
Authors: Tang Hanmin , Wang Jing , Luo Xuehui , Wang Qi , Chen Jie , Zhang Xinyue , Li Qiuting , Gao Chengyi , Li Yuesen , Han Suxia
Published: December 2021

Purpose: To construct an apoptosis-related gene prognostic index (ARGPI) for colon cancer, and clarify the molecular and immune characteristics of the risk subgroup as defined by the prognostic index and the benefits of adjuvant chemotherapy. Integrating the prognostic index and clinicopathological risk factors to better evaluate the prognosis of patients with colon cancer.

Methods: Based on the colon adenocarcinoma data in the TCGA database, 20 apoptosis-related hub genes were screened by weighted gene co-expression network analysis (WGCNA). Five genes constituting the prognosis model were determined by Cox regression and verified by the Gene Expression Omnibus (GEO) dataset. Then the molecular and immune characteristics of risk subgroups defined by the prognostic index and the benefits of adjuvant chemotherapy were analyzed. Finally, nomograms integrating ARGPI and four clinicopathological risk factors were used to evaluate the prognosis of patients with colon cancer.

Results: The ARGPI was constructed based on the FAS, VWA5A, SPTBN2, PCK1, and TIMP1 genes. In the TCGA cohort, patients in the low-risk subgroup had a longer progression-free interval (PFI) than patients in the high-risk subgroup, which coincided with the results of the GEO cohort. The comprehensive results showed that the high-risk score was related to the enrichment of the cell cycle pathway, high mutation rate of TP53 and KRAS, high infiltration of T regulatory cells (Tregs), immunosuppressive state, and less chemotherapeutic benefit. However, low-risk scores are related to drug metabolism-related pathways, low TP53 and KRAS mutation rates, high infiltration of plasma cells, more resting CD4 memory cells and eosinophils, active immune function, and better chemotherapeutic benefits. Receiver operating characteristic curve of two-year progress prediction evaluation showed that the ARGPI had higher prognostic accuracy than TNM staging. Nomograms integrating ARGPI and clinicopathological risk factors can better evaluate the prognosis of patients with colon cancer.

Conclusions: The ARGPI is a promising biomarker for determining risk of colon cancer progression, molecular and immune characteristics, and chemotherapeutic benefit. This is a reliable method to predict the prognosis of colon cancer patients. It also can assist doctors in formulating more effective treatment strategies.
Assessment of CMIP6 Model Performance for Wind Speed in China
Authors: Zhao Lijun , Jin Shuanglong , Liu Xiaolin , Wang Bo , Song Zongpeng , Hu Ju , Guo Yuyang
Published: December 2021

As the major renewable energy, wind can greatly reduce carbon emissions. Following the “carbon neutral” strategy, wind power could help to achieve the realization of energy transformation and green development. Based on ERA5 reanalysis data and the multi-ensemble historical and scenario simulations of the Coupled Model Intercomparison Project Phase 6 (CMIP6), a variety of statistical analyses are used to evaluate the performance of CMIP6 simulating the wind speed in China. The conclusions are as follows: spatial patterns of the nine CMIP6 models are similar with ERA5, but BCC-CSM2-MR and MRI-ESM2-0 highly overestimate the wind speed in northwest China. CESM2-WACCM, NorESM2-MM, and HadGEM3-GC31-MM behave better than the other six CMIP6 models in four specific regions are chosen for detailed study. CESM2-WACCM, NorESM2-MM, and HadGEM3-GC31-MM tend to simulate a larger wind speed than ERA5 except the yearly averaged wind speed in region II and region IV. CESM2-WACCM and NorESM2-MM simulate a large monthly mean wind speed, but the value is relatively close with ERA5 in the summer. HadGEM3-GC31-MM overestimates wind speed in region I and region II from April to October, but gets closer with ERA during winter. CESM2-WACCM, NorESM2-MM, and HadGEM3-GC31-MM simulate an increasing trend in Tibetan Plateau and Xinjiang in the next 100 years, while NorESM2-MM projects rising wind speed in the eastern part of Inner Mongolia, and HadGEM3-GC31-MM simulates increasing wind speed in the northeast and central China. The future wind speed in three models is projected to decline in region I, and the value of HadGEM3-GC31-MM is much larger. In region II, wind speed simulated by three models is projected to decrease, but the wind speed from HadGEM3-GC31-MM in region III and modeled wind speed in region IV from NorESM2-MM would climb with the slope equal to 0.0001 and 0.0012, respectively. This study indicates that the CMIP6 models have certain limitations to perform realistic wind changes, but CMIP6 could provide available reference for the projection of wind in specific areas.
Reflections on Enhancing the Impact of Climate Risk Management Through Transformative Adaptation
Authors: Hellin Jon , Fisher Eleanor , Loboguerrero Ana María
Published: December 2021

Climate risk management is part of the response to the threat of climate change. Much effort has focused on the promotion on climate-resilient agriculture. There continues to be undue focus on technology solutions per se and not enough attention on the coupling of technologies and socio-economics and how they become embedded in ecological systems underpinning smallholder agriculture. In this perspective, we argue that an intertwined social–ecological–technological systems approach to climate risk management is needed to ensure that climate-resilient agriculture contributes more to the realization of goals of the Paris Agreement and the UN Framework Convention on Climate Change. Furthermore, in line with broader policy thinking on the need for transformative change toward sustainably living on the planet and “leaving no one behind,” a greater focus on transformative adaptation is required. Transformative adaptation tackles the root causes of vulnerability including unevenly distributed power relations, and extant networks of control and influence. There are, however, relatively few examples of moving from the theory of transformative adaptation to practice. Three recent practical examples of transdisciplinary approaches, that we have direct experience of as researchers, provide lessons for initial ways forward as part of climate risk management initiatives. Examples from Vietnam, East and Southern Africa, and Guatemala illustrate the importance of inter- and transdisciplinary responses whereby the inequalities underlying unequal power structures may be addressed, enabling farmers to pursue climate risk management pathways that contribute to climate resilience and human development, as epitomized by the Sustainable Development Goals.
Implementing Machine Learning in Interventional Cardiology: The Benefits Are Worth the Trouble
Authors: Ben Ali Walid , Pesaranghader Ahmad , Avram Robert , Overtchouk Pavel , Perrin Nils , Laffite Stéphane , Cartier Raymond , Ibrahim Reda , Modine Thomas , Hussin Julie G.
Published: December 2021

Driven by recent innovations and technological progress, the increasing quality and amount of biomedical data coupled with the advances in computing power allowed for much progress in artificial intelligence (AI) approaches for health and biomedical research. In interventional cardiology, the hope is for AI to provide automated analysis and deeper interpretation of data from electrocardiography, computed tomography, magnetic resonance imaging, and electronic health records, among others. Furthermore, high-performance predictive models supporting decision-making hold the potential to improve safety, diagnostic and prognostic prediction in patients undergoing interventional cardiology procedures. These applications include robotic-assisted percutaneous coronary intervention procedures and automatic assessment of coronary stenosis during diagnostic coronary angiograms. Machine learning (ML) has been used in these innovations that have improved the field of interventional cardiology, and more recently, deep Learning (DL) has emerged as one of the most successful branches of ML in many applications. It remains to be seen if DL approaches will have a major impact on current and future practice. DL-based predictive systems also have several limitations, including lack of interpretability and lack of generalizability due to cohort heterogeneity and low sample sizes. There are also challenges for the clinical implementation of these systems, such as ethical limits and data privacy. This review is intended to bring the attention of health practitioners and interventional cardiologists to the broad and helpful applications of ML and DL algorithms to date in the field. Their implementation challenges in daily practice and future applications in the field of interventional cardiology are also discussed.
The Association and Pathogenesis of SERPINA3 in Coronary Artery Disease
Authors: Li Bo , Lei Zhijun , Wu You , Li Bingyu , Zhai Ming , Zhong Yuan , Ju Peinan , Kou Wenxin , Shi Yefei , Zhang Xianling , Peng Wenhui
Published: December 2021

Background: Serine proteinase inhibitor A3 (SERPINA3) has been discovered in the pathogenesis of many human diseases, but little is known about the role of SERPINA3 in coronary artery disease (CAD). Therefore, we aim to determine its relationship with CAD and its function in the pathogenesis of atherosclerosis.

Methods: In total 86 patients with CAD and 64 patients with non-CAD were compared. The plasma SERPINA3 levels were measured using ELISA. Logistic regression analysis and receiver-operating characteristic (ROC) analysis were performed to illustrate the association between plasma SERPINA3 levels and CAD. In vitro, real-time PCR (RT-PCR) and immunofluorescence staining were used to determine the expression of SERPINA3 in atherosclerotic plaques and their component cells. Then rat aortic smooth muscle cells (RASMCs) were transfected with siRNA to knock down the expression of SERPINA3 and human umbilical vein endothelial cells (HUVECs) were stimulated by SERPINA3 protein. EdU assay and scratch assay were used for assessing the capability of proliferation and migration. The cell signaling pathway was evaluated by western blot and RT-PCR.

Results: Patients with CAD [104.4(54.5–259.2) μg/mL] had higher levels of plasma SERPINA3 than non-CAD [65.3(47.5–137.3) μg/mL] (P = 0.004). After being fully adjusted, both log-transformed and tertiles of plasma SERPINA3 levels were significantly associated with CAD. While its diagnostic value was relatively low since the area under the ROC curve was 0.64 (95% CI: 0.55–0.73). Secreted SERPINA3 might increase the expression of inflammatory factors in HUVECs. Vascular smooth muscle cells had the highest SERPINA3 expression among the aorta compared to endothelial cells and inflammatory cells. The knockdown of SERPINA3 in RASMCs attenuated its proliferation and migration. The phosphorylated IκBα and its downstream pathway were inhibited when SERPINA3 was knocked down.

Conclusions: Elevated plasma SERPINA3 levels were associated with CAD. SERPINA3 can increase inflammatory factors expression in HUVECs. It can regulate VSMCs proliferation, migration, and releasing of inflammatory factors through the NF-κB signaling pathway. Thus, SERPINA3 played a significant role in the pathogenesis of atherosclerosis.
The Present State and Future Perspectives of Cardiac Regenerative Therapy Using Human Pluripotent Stem Cells
Authors: Soma Yusuke , Morita Yuika , Kishino Yoshikazu , Kanazawa Hideaki , Fukuda Keiichi , Tohyama Shugo
Published: December 2021

The number of patients with heart failure (HF) is increasing with aging in our society worldwide. Patients with HF who are resistant to medication and device therapy are candidates for heart transplantation (HT). However, the shortage of donor hearts is a serious issue. As an alternative to HT, cardiac regenerative therapy using human pluripotent stem cells (hPSCs), such as human embryonic stem cells and induced pluripotent stem cells, is expected to be realized. Differentiation of hPSCs into cardiomyocytes (CMs) is facilitated by mimicking normal heart development. To prevent tumorigenesis after transplantation, it is important to eliminate non-CMs, including residual hPSCs, and select only CMs. Among many CM selection systems, metabolic selection based on the differences in metabolism between CMs and non-CMs is favorable in terms of cost and efficacy. Large-scale culture systems have been developed because a large number of hPSC-derived CMs (hPSC-CMs) are required for transplantation in clinical settings. In large animal models, hPSC-CMs transplanted into the myocardium improved cardiac function in a myocardial infarction model. Although post-transplantation arrhythmia and immune rejection remain problems, their mechanisms and solutions are under investigation. In this manner, the problems of cardiac regenerative therapy are being solved individually. Thus, cardiac regenerative therapy with hPSC-CMs is expected to become a safe and effective treatment for HF in the near future. In this review, we describe previous studies related to hPSC-CMs and discuss the future perspectives of cardiac regenerative therapy using hPSC-CMs.
AquaScouts: ePROs Implemented as a Serious Game for Children With Cancer to Support Palliative Care
Authors: Hoffmann Stefan , Schraut Robert , Kröll Thomas , Scholz Wiebke , Belova Tatiana , Erhardt Johann , Gollmer Daniel , Mauck Christian , Zacharioudakis Giorgos , Meyerheim Marcel , Bonotis Panos , Kakalou Christine , Chatzimina Maria , Karamanidou Christina , Sander Annette , Didi Jana , Graf Norbert , Natsiavas Pantelis
Published: December 2021

MyPal is a European initiative focusing on the use of the electronic patient reported outcome (ePRO) measures to enhance patient engagement in palliative cancer care via digital self-reporting palliative care for patients with cancer. As a part of its approach, MyPal also focuses on pediatric patients, implementing a specific digital health platform including a serious game to facilitate the reporting of the symptoms and overall status regarding their quality of life (QoL). To this end, the reduction of psychological burden related to frequent reporting, a.k.a. as “reporting fatigue” has been identified as a priority. In this study, we present the MyPal-CHILD platform, emphasizing on the serious game named AquaScouts and its key design decisions, while also emphasizing on the respective challenges. More specifically, we provide insights on the participatory design approach applied during the design of the platform and the high-level goals defined based on end-user input. In addition, the validation process applied before the use of the platform under real-world conditions is also presented. Finally, we discuss a number of challenges and the prospects of deploying eHealth interventions to support palliative care.
Switching Intent of Disruptive Green Products: The Roles of Comparative Economic Value and Green Trust
Authors: Lin Chunpei , Lai Xiumei , Yu Chuanpeng
Published: December 2021

This study explores consumers’ motivations to switch to new products in the context of disruptive innovation and investigates the role of comparative economic value and green trust. Switching from an existing product to a disruptive green product not only involves benefits but also requires major sacrifices, which are not encountered in the context of continuous innovation. In this study, the relationships between comparative economic value, green trust, self-accountability, and disruptive green product switching intent are examined. Data were collected from China with self-administered questionnaires regarding the disruptive green product. Results of a structural model reveal positive relationships between comparative economic value, green trust, and disruptive green product switching intent. In addition, green trust mediates the effects of the comparative economic value on the disruptive green product switching intent, and self-accountability moderates the relationship between green trust and disruptive green product switching intent. From a practitioner perspective, the research is important because it illuminates the consumer’s motivations regarding product switching in the hitherto unexplored field of automobiles, for which we have shown that our extended model yields meaningful results.
Repercussions of Sustainable Agricultural Productivity, Foreign Direct Investment, Renewable Energy, and Environmental Decay: Recent Evidence from Latin America and the Caribbean
Authors: Naseem Sobia , Hui Wang , Sarfraz Muddassar , Mohsin Muhammad
Published: December 2021

This research aimed to assess and implement the long- and short-run relationship of agriculture and environmental sustainability with control variables. Purposely, this research consolidated theoretical and conceptual principles to create a systematic structure in agriculture for the development of both sectors, i.e., agricultural and the environment. On this ground statement, this research was motivated to contemplate the relationship between carbon dioxide emission, agricultural production, gross domestic product, renewable energy consumption, and foreign direct investment using annual data series of Latin American and Caribbean countries from 1971 to 2018. Autoregressive distributed lag (ARDL) was used as an econometric methodology to examine the relationship among the variables. Agriculture is the most vulnerable sector in Latin American and Caribbean countries, and the economy is heavily dependent on it. The main results of this research indicated that agriculture and CO2 emissions were positively related to each other for the long and short run, which means that agricultural activities increased the CO2 emission levels. At the same time, the control variables showed mixed associations with environmental degradation as gross domestic product (GDP) was positively significant and renewable energy consumption was negatively significant. The error correction (ECt−1) term was negatively significant, confirming the long-run relationship and the speed of adjustment from short- to long-run equilibrium. Agricultural production and GDP led to increments in CO2 emissions, while renewable energy consumption negatively contributed to toxic emissions. The speed of adjustment in Latin American and Caribbean countries was nippy. It required 2.933 periods for the transformation from the short periodic phase to the long term. A comprehensive approach is the research debate rigorously and holistically based on divergent sectors of an economy and their relationship with environmental sustainability. The econometric method, symbolic system, and conceptual existence were designed originally.