bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Farming Systems, Genotype and Their Interaction on Bioactive Compound, Protein and Starch Content of Bread and Spelt Wheat
Authors: Takač Verica , Tóth Viola , Rakszegi Marianna , Mikó Péter , Mikić Sanja , Mirosavljević Milan
Published: December 2022

An increase in the production and consumption of spelt products can be associated with positive effects on human health, which are attributed to bioactive compounds present in the grain. The basic success of spelt wheat in organic farming might be explained by the fact that spelt wheat belongs to the group of hulled wheat where the presence of a husk protects the seed from abiotic and biotic stress factors, thus demanding less chemical protection. The goal of this study was to investigate the variations in the bioactive compound (alkylresorcinol, arabinoxylan, β-glucan), protein, starch and fructan content of bread and spelt wheat under different farming systems (conventional and organic). The results showed higher protein and alkylresorcinol but lower fructan content in spelt wheat. Organic spelt had significantly higher starch, fiber and alkylresorcinol content but lower β-glucan and protein content than conventionally grown spelt. The spelt variety ‘Oberkulmer-Rotkorn’ was characterized by the highest values for the majority of analyzed traits under both farming systems. Overall, the environmental conditions (Hungary and Serbia), farming systems (conventional and organic) and wheat species (bread and spelt) contributed to the variations of the compositional traits in different manners.
Characterization of the Quality and Oxidative Stability of Hemp-Oil-Based Oleogels as an Animal Fat Substitute for Meat Patties
Authors: Hamidioglu Irfan , Alenčikienė Gitana , Dzedulionytė Miglė , Zabulionė Aelita , Bali Aditya , Šalaševičienė Alvija
Published: December 2022

The effect of the incorporation of rice bran wax (5%; 7%) or candelilla wax (3%; 7%) for production of hemp-oil-based oleogels was analyzed in this study. The experiment was carried out to replace between 0 and 100% of animal fat in meat patties with oleogels. Free fatty acids (FFAs), acid value (AV), oxidative stability index (OSI), conjugated diene value, malondialdehyde value, physicochemical properties, and the sensory properties of oleogels and meat patties were studied. The results indicated that hemp oil had more polyunsaturated fatty acids and lower oxidative stability when compared to oleogels. The OSI for oil was 3.1 h, while for oleogels it was 3.4–3.6 (candelilla case) or 3.7–3.9 (rice bran). Oleogels were able to match pork fat texture properties such as spreadability and adhesiveness in meat patties. However, sensory data for cooked meat patties with animal fat fully replaced by oleogels revealed that samples with 100% pork fat had higher juiciness and taste intensity. Our results showed that a wax-based oleogel had a higher oxidative stability and nutritional profile, but further investigations to mimic pork fat properties in meat patties are necessary.
Effect of Mulberry Leaf TMR on Growth Performance, Meat Quality and Expression of Meat Quality Master Genes (ADSL, H-FABP) in Crossbred Black Goats
Authors: Long Yong , Han Yong , Zhao Yuanfeng , Chen Dianqian , Wang Defeng , Yang Yang , Su Chaozhi , Shen Xiaoyun
Published: December 2022

This study was conducted to examine the effect of a mulberry leaf total mixed ration (TMR) diet on growth performance, apparent digestibility, meat quality and the expression of related meat-quality genes (ADSL, H-FABP) in crossbred black goats. Forty-four Guizhou crossbred black goats (Nubian black goat ♂ × Guizhou black goat ♀), weighing 33.43 ± 0.55 kg, were chosen. The goats were randomly divided into four groups, with 11 test replicates in each group. Group I was the control group and fed with the traditional feeding method of roughage and concentrate supplement without adding mulberry leaf. Group II was fed with a 40% mulberry leaf pellet TMR diet. Group III was fed with a freshly processed 40% mulberry leaf TMR diet. Group IV was fed with a 40% mulberry leaf fermented total mixed rations (FTMR) diet. The results showed that the average daily gain (ADG) of group II was significantly higher than that of group I and III (p < 0.05). The apparent digestibility of group II of ether extract (EE) and neutral detergent fiber (NDF) was significantly higher than that of group I (p < 0.05), and the apparent digestibility of dry matter (DM) and crude protein (CP) was significantly higher than that of group I (p < 0.01). Compared with group I, meat in group II had lower meat color lightness (L*) and yellowness (b*) values (p < 0.01) in the Longissimus thoracis et lumborum. The shear force of group II was significantly lower than that of group I (p < 0.05). The total fatty acids (TFA) of group II was significantly higher than that of groups I and III (p < 0.05), but the total saturated fatty acids (SFA) of group II was significantly lower that than of group I (p < 0.01). Subsequently, the Unsaturated fatty acids (USFA), Monounsaturated fatty acids (MUFA), and Polyunsaturated fatty acids (PUFA) of group II were significantly higher than those in group I (p < 0.01). The contents of total amino acids (TAA), total essential amino acids (EAA), total non-essential amino acids (NEAA) and total of major fresh-tasting amino acids (DAA) of groups II, III and IV were significantly higher than those of group I (p < 0.05), as well as the contents of IMP (p < 0.01). The expression of the H-FABP gene in the arm triceps of group II was significantly higher than that of groups I, III and IV (p < 0.05). The expression of the ADSL gene in the Longissimus thoracis et lumborum and biceps femoris of group II was significantly higher than that of group I (p < 0.05). Collectively, the results of the current study indicated that the mulberry leaf TMR diet improved the growth performance, apparent digestibility and expression of related meat-quality master genes (ADSL, H-FABP) in crossbred black goats, which promoted the deposition of intramuscular fat (IMF) and inosinic acid (IMP) and improved the composition of fatty acids and amino acids in the muscles.
Pasta from Red Lentils (Lens culinaris): The Effect of Pasta-Making Process on Starch and Protein Features, and Cooking Behavior
Authors: Bresciani Andrea , Erba Daniela , Casiraghi Maria Cristina , Iametti Stefania , Marti Alessandra , Barbiroli Alberto
Published: December 2022

The effect of pasta-making processes on starch and protein features, as well as cooking behavior, and nutritional properties (i.e., resistant starch and starch in vitro digestibility) were assessed. Pasta from raw red lentils (R) was prepared by conventional extrusion (C_R) and extrusion-cooking (EC_R), whereas heat-treated red lentils (HT) were processed into pasta by conventional extrusion (C_HT). A “high protein” and “high fiber” pasta was prepared. Using HT was effective in increasing the luminosity (that was about 88, 91, and 96 for EC_R, C_R, and C_HT, respectively), and decreasing the presence of defects on the pasta surface (heterogeneity was 5%, 36%, and 45% for C_HT, EC_R, and C_R, respectively). Heat treatment on grains or flour significantly increased starch susceptibility to α-amylase (6.6, 7.4, and 8.6% for C_R, C_HT, and EC_R, respectively) and decreased the final viscosity (from 335 BU in C_R to 287 and 291 BU in EC_R and C_HT), resulting in a significant increase in starch digestibility (slowly digestible starch was about 41, 27, and 26% in C_R, C_HT, and EC_R, respectively). As regards proteins, the main effect on their structure was observed in C_HT, where the cooking behavior was much improved and cooking losses were lowest (5.7%). On the other hand, protein and starch organization in EC_R might have accounted for pasta resistance in overcooking.
Liouville Type Theorems Involving the Fractional Laplacian on the Upper Half Euclidean Space
Authors: Zhang Tao
Published: December 2022

In this paper, we mainly establish Liouville-type theorems for the elliptic semi-linear equations involving the fractional Laplacian on the upper half of Euclidean space. We employ a direct approach by studying an equivalent integral equation instead of using the conventional extension method. Applying the method of moving planes in integral forms, we prove the non-existence of positive solutions under very weak conditions. We also extend the results to a more general equation.
Assessment of Some Clay-Based Products Available on Market and Designed for Topical Use
Authors: Bastos Carla Marina , Rocha Fernando
Published: December 2022

The establishment of quality requirements of clay-based products, for medicinal, wellness, and aesthetic purposes, is mainly sustained by the good interactions between the clay-based formulation and the skin. The release of ionizable elements and their availability to percutaneous absorption should be, ideally, physiologically effective during passive percutaneous absorption. Clay-based products are promoted in the European market as therapeutic clays or aesthetics, which is labeling that combines characteristics of medicinal products along with cosmetics. Different countries regulate these products under different legal frameworks. This study focuses on the mineralogical, chemical, and technological characterization of some clay-based products available on the market, designed for topical use, framed in the peloids concept, and claimed as natural products. The main goals are to contribute to the establishment of clay-based products quality criteria as reliable scientific information, aiming for the compliance of intended use, the information for the potential health hazards and toxicological effects of clay-based products, and the distinction in what concerns therapeutic compliance and aesthetic or wellbeing product certification. There were 13 clayed products for cosmetic purposes, available online and in commercial stores, together with three thermal peloids, that were studied. Mineralogical composition of the 16 studied samples reveals a polymineralic association with the presence of variable quantities of quartz, calcite, and feldspars, whereas clay minerals are not predominant and characterized by the presence of clay-based fraction content, composed mainly by illite, smectite, and kaolinite in variable amounts and with several mineral associations. The clay-based products contain median values of 17 ppm As, 315 ppm Ba, 79 ppm Cr, 11 ppm Co, 29 ppm Pb, 26 ppm Ni, and 62 ppm Zn. One sample presented 4.1 ppm of Cd. The studied samples have safety concerns about specific limits of As, Ba, Cd, Cr, Co, Pb, Ni, and Zn which are above the regulated avoidable limits. Samples’ pH is out of range of skin’s natural pH as well.
Application of Prenatal Whole Exome Sequencing for Structural Congenital Anomalies—Experience from a Local Prenatal Diagnostic Laboratory
Authors: Lai Theodora Hei Tung , Au Leung Kuen Sandy , Lau Yuen Ting Eunice , Lo Hei Man , Chan Kelvin Yuen Kwong , Cheung Ka Wang , Ma Teresa Wei Ling , Leung Wing Cheong , Kong Choi Wah , Shu Wendy , So Po Lam , Kwong Anna Ka Yee , Mak Christopher Chun Yu , Lee Mianne , Chui Martin Man Chun , Chung Brian Hon Yin , Kan Anita Sik Yau
Published: December 2022

Fetal structural congenital abnormalities (SCAs) complicate 2–3% of all pregnancies. Whole-exome sequencing (WES) has been increasingly adopted prenatally when karyotyping and chromosomal microarray do not yield a diagnosis. This is a retrospective cohort study of 104 fetuses with SCAs identified on antenatal ultrasound in Hong Kong, where whole exome sequencing is performed. Molecular diagnosis was obtained in 25 of the 104 fetuses (24%). The highest diagnostic rate was found in fetuses with multiple SCAs (29.2%), particularly those with involvement of the cardiac and musculoskeletal systems. Variants of uncertain significance were detected in 8 out of the 104 fetuses (7.7%). Our study shows the utility of WES in the prenatal setting, and the extended use of the technology would be recommended in addition to conventional genetic workup.
Association between Breast Cancer Knowledge and Mammogram Utilization among Immigrant Muslim Arab Women in California: Cross-Sectional Design
Authors: Alkhaifi Sarah , Badr Hanan
Published: December 2022

Background: Regular mammogram screenings have contributed to early breast cancer (BC) diagnoses and lowered the mortality rate by 40% in the United States of America (USA). Nonetheless, ethnic women living in developed countries, such as immigrant Muslim Arab women (IMAW), are less likely to get mammograms. Aim of the study: In our study, we aimed to understand health behaviors among IMAWs as understudied populations in the USA. Methods: We conducted a cross-sectional study on a convenience sample of IMAW living in southern California. We used logistic regression and multivariate logistic regressions to analyze the data. Results: The total number of participants who completed the survey was 184 IMAW. Participants who had a higher level of knowledge about BC signs and symptoms and mammogram knowledge were more likely to have obtained a mammogram at some point compared with their counterparts (OR = 1.23, p = 0.03, CI: 1.07–1.42; OR = 2.23, p = 0.23, CI: 1.11–4.46, respectively). Conclusions: Our results provide more evidence emphasizing the important influence of BC and mammogram knowledge on immigrant women’s behavior regarding mammogram utilization. The average level of knowledge in all three domains (BC risk factors, BC signs and symptoms, and mammogram use) reported in this study is considered low.
Differentiating between Natural and Modified Cellulosic Fibres Using ATR-FTIR Spectroscopy
Authors: Geminiani Ludovico , Campione Francesco , Corti Cristina , Luraschi Moira , Motella Sila , Recchia Sandro , Rampazzi Laura
Published: December 2022

This paper presents the limitations and potential of ATR-FTIR spectroscopy applied to the study of cellulosic textile collections. The technique helps to differentiate natural fibres according to the content of lignin, pectin, hemicellulose, and wax, although some problematic issues should be considered. The spectral differences derived from the environmental humidity uptake and the plant composition are reviewed and discussed in the light of new experimental data. Diagnostic bands are proposed that can discriminate between different fibres from different plants. The contribution of ageing is also considered, demonstrating that sometimes aged fibres cannot be reliably recognised. In contrast, the potential of ATR-FTIR spectroscopy to discriminate between natural and modified fibres is discussed and proven. The best results were obtained when microinvasive ATR-FTIR spectroscopy was coupled with SEM observations. The proposed protocol was tested on microsamples of various cellulosic materials from traditional Japanese samurai armours dating from the 16th to the 20th centuries (Morigi Collection, Museo delle Culture, Lugano, Switzerland). The results facilitated a complete characterisation of the materials and demonstrated that the protocol can be used to study a wide variety of cellulosic materials, including both natural and man-modified fibres, and paper.
Effects of a Long-Term Adapted Judo Program on the Health-Related Physical Fitness of Children with ASD
Authors: Pierantozzi Emanuela , Morales José , Fukuda David H. , Garcia Vanessa , Gómez Antonia M. , Guerra-Balic Myriam , Carballeira Eduardo
Published: December 2022

Physical fitness is one of the most important physical and mental health aspects for children with Autism Spectrum Disorder (ASD). This study aimed to test the effects of a long-term adapted judo program on the health-related physical fitness of children with ASD. The participants were recruited from various associations of families and schools for children with special needs. Twenty-one children were assigned to an experimental group and nineteen to a control group. The experimental group participated in a six-month adapted judo program consisting of 90 min of practice each week. Health-related physical fitness was measured using the indicators obtained from the ALPHA-fitness battery, the estimated VO2max and the waist/height ratio0.5. Changes within and between groups were analyzed using linear mixed models for repeated measures designs and test-retest reliability of tests requiring a maximum score using the Intraclass Correlation Coefficient (ICC). A judo program tailored for children with ASD can improve the cardio-metabolic health and cardiorespiratory fitness of its participants. The problems involved with administering physical aptitude tests that involve maximum effort or performance in children with ASD cast serious doubts on the reproducibility of their results.
“Virtual Surf Booth”: Assessment of a Novel Tool and Data Collection Process to Measure the Impact of a 6-Week Surf Programme on Mental Wellbeing
Authors: Gerami Ariane , Foster Charlie , Murphy Joey
Published: December 2022

Surf therapy is increasingly used as a health intervention, but evidence of its mental health benefits remains unclear. This longitudinal mixed-method study assessed the usability and acceptability of a novel online data collection tool and process to measure the impact of a surf programme on acute and chronic mental wellbeing. Fifteen women attending a 6-week surf programme in the UK were asked to complete a tool consisting of video recordings, word association and the Short Warwick-Edinburgh Mental Well-Being scale (SWEMWBS). Usability and acceptability were assessed through focus groups and quantitative data. The data generated in the focus groups, video recordings and word association were analysed via reflexive thematic analysis, and SWEMWBS presented descriptively. Participants perceived the tool as easy to use due to the completion time and its functionalities, and useful for self-reflection. Facilitating conditions such as timing and location, areas for improvement such as increased privacy, accessibility, incentivisation, and factors impacting data generated were further identified. Data collected covered both acute and chronic mental wellbeing and showed a positive relationship between surf and mental wellbeing. Further research is needed to confirm these findings in diverse populations, identify potential moderators, and confirm the validity of this tool and process.
Assessment of the Health Care System in Poland and Other OECD Countries Using the Hellwig Method
Authors: Smarżewska Daria , Wereda Wioletta Sylwia , Jończyk Joanna Anna
Published: December 2022

The health care system is a key element in the functioning of any country. However, depending on the level of funding, the number of medical staff and their availability, there are significant discrepancies in the health care systems of different countries. This article presents a picture of the Polish health care system compared to the systems of other selected OECD countries. The comparison was made on the basis of selected indicators concerning financing, medical and nursing staff as well as patient satisfaction with the availability and quality of healthcare. The aim of this article is to analyze the Polish health care system and compare it with other selected OECD countries’ health care systems. A literature review, secondary data analysis and statistical analyses were used as the research method. The ranking was prepared using Hellwig’s linear ordering method. Ten indicators related to financing, medical and nursing staff, and residents’ opinions on the availability and quality of medical services were selected for the analysis. The presented results clearly indicated that Norway, Germany and Switzerland have the best health care systems. The Polish system takes one of the last places in developed classification. The conducted analysis indicates the need to introduce changes to the health care system and the need to implement solutions from countries where health care systems have been indicated as the best.
Research on the Driving Factors of Collective Nostalgia and the Impact of Collective Nostalgia on National Brand Consciousness
Authors: Zhang Yi , Zhou Hang , Qin Jian
Published: December 2022

Nostalgia is an important factor affecting consumers’ intention and behavior. A lot of previous research on nostalgia has been conducted from the perspective of individuals rather than groups. Then how does group-based collective nostalgia come into being? How will consumers’ collective nostalgia affect their consumption decisions? And what can we do to guide it? By sorting out the relevant literature, this paper attempts to explore the driving factors of collective nostalgia and observe the internal impact of it on national brand consciousness. Furthermore, a mechanism model of collective nostalgia is constructed, and data collection and empirical analysis are carried out by means of a questionnaire. The results show that relative deprivation, social alienation, interpersonal alienation and environmental alienation have significant positive predictive effects on collective nostalgia, while cultural discontinuity and historical discontinuity have no significant predictive effect on collective nostalgia. In addition, collective nostalgia has a positive influence on national brand consciousness; personal identity, social identity and collective identity all play mediating roles between collective nostalgia and national brand consciousness. With the improvement in social emotion, the positive effect of social identity and collective identity on national brand consciousness is strengthened, while the influence of personal identity on national brand consciousness is not significant. The study enriches the basic theory of collective nostalgia and national brand consciousness and provides suggestions for further developing domestic brands and expanding the influence of domestic brands.
Magnetic-Transition-Metal Oxides Modified Pollen-Derived Porous Carbon for Enhanced Absorption Performance
Authors: Tai Shuyun , Li Ying , Yang Ling , Zhao Yue , Wang Sufei , Xia Jianxin , Li Hua
Published: December 2022

In our work, the transition-metal-oxide precursor (TMO@BC, M = Fe, Co, Ni) has been loaded on the pollen carbon by the hydrothermal method and annealed at different temperatures to generate a composite material of metal oxide and pollen carbon in this study, which can effectively prevent agglomeration caused by a small size and magnetism. The XRD patterns of the samples showed that the as-synthesized metal oxides were γ-Fe2O3, CoO, and NiO. In the 20 mg/L methyl orange adsorption experiment, the adsorption amount of CoO@C at 500 ℃ reached 19.32 mg/g and the removal rate was 96.61%. Therefore, CoO@C was selected for the adsorption correlation-model-fitting analysis, which was in line with the secondary reaction. The pseudo-second-order kinetic model (R2: 0.9683–0.9964), the intraparticle diffusion model, and the Freundlich adsorption isotherm model indicated that the adsorption process was the result of both physical and chemical adsorptions, and the judgment was based on the electrostatic action. The adsorption and removal efficiency of ciprofloxacin (CIP) by changing the pH of the reaction was about 80%, so the electrostatic attraction worked, but not the main factor. Recovered by an external magnetic field, the three-time recycling efficiency was still maintained at more than 80%. This novel biomass-derived magnetic porous carbon material embedded with transition-metal-oxide nanoparticles is highly promising for many applications, especially in the field of environmental remediation.
1000 Days: The “WeCare Generation” Program—The Ultimate Model for Improving Human Mental Health and Economics: The Study Protocol
Authors: Uccellini Orlando , Benlodi Andrea , Caroppo Emanuele , Cena Loredana , Esposito Gianluca , Fernandez Isabel , Ghazanfar Maria , Imbasciati Antonio , Longo Francesco , Mazza Marianna , Marano Giuseppe , Nacinovich Renata , Pignatto Antonio , Rolnick Arthur , Trivelli Marco , Spada Elena , Vanzini Cinzia
Published: December 2022

Introduction: The COVID-19 pandemic stressed the necessity of a new resilience of the human population and health system. The “WeCare Generation” program is a new proposal of territorial intervention, with a new paradigm, on the diseases of the human body and mind. Background: In recent decades, the independent strands of investigation on brain plasticity and early trauma consequences have demonstrated that traumatic experiences in the period from pregnancy to the age of 3 years have an enormous impact on an individual’s future development, and both physical and mental health. Research shows that adverse child experiences (ACEs) are associated with a strong risk of conditions such as: harmful alcohol use, smoking, illicit drug use, high body-mass index, depression, anxiety, interpersonal violence, cancer, type 2 diabetes, cardiovascular diseases, stroke respiratory diseases and, as a consequence, to a high financial cost in Italy and also across Europe (1–9% GDP) and the USA (total annual costs estimated to be USD 581 billion in Europe and USD 748 billion in North America). All this suggests that an early intervention on that traumatized-slice of population leads to multiplied savings. Methods: A multi-center, randomized, controlled trial was designed. The parents of the future neonatal population (from pregnancy to delivery) with trauma will be enrolled, and randomized to treatment, or control arm. The article describes in detail how the primary outpoint (cost to the national health system), and some secondary outpoints, will be collected. Discussion: An overall rate of return on investment (ROI) statistically significant 13.0% per annum with an associated benefit/cost ratio (BCR) of 6.3 is expected as the primary outcome of the “WeCare Generation” program. Our proposed model predicts a new medical paradigm aiming to empower new generations, with a strong return on economy and health.
Clinical Influence of Mandibular Flexure on Oral Rehabilitation: Narrative Review
Authors: Mijiritsky Eitan , Shacham Maayan , Meilik Yuval , Dekel-Steinkeller Michal
Published: December 2022

Aim: The current paper aims to review mandibular flexure and its clinical implications in the field of oral rehabilitation. Mandibular flexure is a deformity of the mandible, which occurs during jaw movements. Methods and Materials: An electronic database search was conducted using the PRISM model, with a total of 49 articles included. Results: Mandibular flexure affects various stages of oral rehabilitation treatments. Effects of mandibular flexure are more significant in periodontal patients, and in implant-supported restorations, compared to natural teeth, due to differences in the force absorption by the periodontal ligament. Various adjustments must be made to the prosthodontic framework to enable long-term survival of the restorative treatments. Conclusions: Dental practitioners should pay attention to the following: (1) digital impressions are preferred over conventional; (2) mouth opening should be kept to a minimum (as possible, up to 10–20 mm) while also avoiding any anterior movements of the mandible (protrusion); (3) the number of abutment teeth should be kept to a minimum; (4) structures in the lower jaw should be splitted; (5) non-rigid connectors should be used to reduce the effort exerted; (6) in periodontal patients, the preference is for short-span restorations and non-rigid connectors; (7) in implant-supported restorations, it is preferable to divide the framework into two or three segments, utilizing rigid materials with a low elastic modulus. There is no agreement in the literature about the preferred location of the implants in the jaw.
Study on the Impact of Environmental Tax on Industrial Green Transformation
Authors: Shen Yang , Zhang Xiuwu
Published: December 2022

Tax revenue is one of the essential means through which the government controls the macro-economy and plays a vital role in promoting environmental protection and sustainable development. This study takes Chinese panel data from 2004 to 2020 as sample observations, uses the SBM-GML index method to measure industrial green total factor productivity, and then uses econometric methods such as the two-way fixed effects model and instrumental variable method to analyze the impact of an environmental tax on industrial green transformation. It is found that the generalized environmental tax represented by vehicle and vessel tax, resource tax, and urban land use tax has a significant positive effect on industrial green transformation. After a series of robustness tests and the exclusion of endogeneity, this conclusion remains valid. The research shows that credit governance, the agglomeration of producer service, and their co-agglomeration with manufacturing are important adjustment mechanisms. Among them, credit management is special and compulsory, greatly restricting the environmental pollution behavior of industrial enterprises, and encourages enterprises to make green investments and to actively improve production processes.
Does BMI Modify the Association between Vitamin D and Pulmonary Function in Children of the Mild Asthma Phenotype?
Authors: Papamichael Maria Michelle , Itsiopoulos Catherine , Katsardis Charis , Tsoukalas Dimitris , Erbas Bircan
Published: December 2022

Vitamin D deficiency and obesity are global health problems that are associated with increased asthma risk in children. The purpose of this study was to investigate whether BMI modifies pulmonary function across vitamin D tertiles in pediatric asthma patients of the mild asthma phenotype. This cross-sectional study conducted from November 2016–September 2017 compared lung function variability as assessed by spirometry and nitric oxide in exhaled breath (FeNO) among 35 normal-weight and 26 overweight/obese Greek schoolchildren (5–12 years old) with mild asthma. Serum 25 (OH)D levels ≥ 30 ng/mL were defined as ‘sufficient’, 20–30 ng/mL ‘insufficient’, and <20 ng/mL ‘deficient’. Stratification by BMI category, linear regression showed positive associations between D, % FVC (β = 0.49, 95%CI: 0.05, 0.94), and % FEV1 (β = 0.48, 95%CI: −0.01, 0.95) in the normal-weight only, adjusted for age, sex, regular exercise, and medication. FEV1 was 10% higher in the normal-weight D-sufficient group compared to those D-deficient (β = 10.43, 95%CI: 0.54, 20.32). No associations were observed for the overweight/obese group or FeNO. In conclusion, BMI modified associations of vitamin D on airway mechanics in children of the mild asthma phenotype. Serum 25 (OH)D concentrations ≥ 30 ng/mL were associated with higher ventilation in central airways of normal-weight asthmatic children.
The Impact of the Available Infrastructure on the Electric Vehicle Market in Poland and in EU Countries
Authors: Tucki Karol , Orynycz Olga , Dudziak Agnieszka
Published: December 2022

The dynamic development of the automotive market entails the need to understand its characteristics. The purpose of this publication is to indicate the changes in the automotive sector resulting from the approaching electromobility. In order to achieve this goal, the market of electric and conventional vehicles in Poland are discussed. This paper discusses issues related to employment in the automotive industry, analyses the location of factories of the largest vehicle manufacturers and analyzes the structure of sales. The development of electromobility in Poland against the background of other EU countries was analyzed in detail. The analysis was based on data from September 2022. The analysis has been conducted by means of computations of the correlation of individual variables (numbers of different types of vehicles: traditional vehicles, BEV and PHEV). The presented results show that the number of BEVs and PHEVs is increasing, and the infra-structure is developing. The number of PHEV cars is usually higher than BEV ones. The results of the analysis of the vehicle market in Poland show occurrence of the variety of vehicle types and variety of the types of vehicle power supplies with alternating current (AC) or direct current (DC). It can be concluded that standardization would be helpful for further development. The number of charging stations is small compared to the number of vehicles, and the advantage of AC stations is evident. This means investing in infrastructure is necessary to enable further development.
Unusual Experience in the COVID-19 Pandemic: Young Children’s Readjustment from Home to Preschool following School Closures in Different Risk Regions
Authors: Hong Xiumin , Zhao Sijie , Liu Qianqian
Published: December 2022

Children’s readjustment to preschool following long-term school closures during the COVID-19 pandemic merits special attention. This study examined children’s preschool readjustment using a survey of 1008 teachers in a high-risk region and 1399 teachers in a fluctuating-risk region of China. Results found are as follows. (1) children’s preschool readjustment was at a medium level after the long-term school closures. However, children’s preschool readjustment scores in the fluctuating-risk region were significantly lower than those in the high-risk region. (2) Children in both regions were divided into four profiles based on their preschool readjustment: low-level, middle-level, upper-middle-level, and high-level groups. (3) Preschool transition practices and teachers’ turnover intention are common factors relating to preschool readjustment in both regions. Teachers’ professional development support impacted children’s preschool readjustment only in the high-risk region. The findings inform the design of targeted interventions to help children readjust to preschool across different risk regions.
Sedentary Behavioral Studies of Young and Middle-Aged Adults with Hypertension in the Framework of Behavioral Epidemiology: A Scoping Review
Authors: Zhang Kexin , Huang Sufang , Feng Danni , Lang Xiaorong , Wang Quan , Liu Yuchen
Published: December 2022

(1) Background: As times change, the detection rate of hypertension is increasing in the young and middle-aged population due to prevalent sedentary behaviors. The purpose of this study was to conduct a scoping review to identify and summarize the research on sedentary behavior in this population by separating it into five stages: the relationship between sedentary behavior and health; measurement modalities; influencing factors; interventions; and translational research in young and middle-aged adults with hypertension. (2) Methods: Using a scoping review research approach, the PubMed, Web of Science Core Collection, and MEDLINE databases were used to search for the literature on this subject from the date of the database’s creation to 14 June 2022, and the behavioral epidemiology framework was used to classify the retrieved articles. (3) Results: A total of eight articles were included. Among them, there were six articles on the relationship between behavior and health, which includes blood pressure, insulin resistance, and the cardiovascular system; one article on the study of measurement methods, which was used for clinical decision making through decision trees; one article on influencing factors, which was divided into intrinsic and extrinsic factors; and no articles on intervention program development or the translation of intervention programs to further practice in this population. (4) Conclusions: Sedentary behavioral studies of young and middle-aged adults with hypertension are scarce and are generally carried out in the early stages of the condition. In the future, in-depth studies can be conducted on the dose–response relationship between sedentary behavior and health in this population; the development of easier and targeted measurement tools; the exploration of more influencing factors; and the effectiveness and translation of intervention programs.
Normal Pregnancy-Induced Islet Beta Cell Proliferation in Mouse Models That Are Deficient in Serotonin-Signaling
Authors: Goyvaerts Lotte , Schraenen Anica , Lemaire Katleen , Veld Peter in’t , Smolders Ilse , Maroteaux Luc , Schuit Frans
Published: December 2022

During mouse pregnancy placental lactogens stimulate prolactin receptors on pancreatic islet beta cells to induce expression of the tryptophan hydroxylase Tph1, resulting in the synthesis and secretion of serotonin. Presently, the functional relevance of this phenomenon is unclear. One hypothesis is that serotonin-induced activation of 5-HT2B receptors on beta cells stimulates beta cell proliferation during pregnancy. We tested this hypothesis via three different mouse models: (i) total Tph1KO mice, (ii) 129P2/OlaHsd mice, which are incompetent to upregulate islet Tph1 during pregnancy, whereas Tph1 is normally expressed in the intestine, mammary glands, and placenta, and (iii) Htr2b-deficient mice. We observed normal pregnancy-induced levels of beta cell proliferation in total Tph1KO mice, 129P2/OlaHsd mice, and in Htr2b−/− mice. The three studied mouse models indicate that islet serotonin production and its signaling via 5-HT2B receptors are not required for the wave of beta cell proliferation that occurs during normal mouse pregnancy.
Expanding the Therapeutic Window of EGFR-Targeted PE24 Immunotoxin for EGFR-Overexpressing Cancers by Tailoring the EGFR Binding Affinity
Authors: Jun Sei-Yong , Kim Dae-Seong , Kim Yong-Sung
Published: December 2022

Immunotoxins (ITs), which are toxin-fused tumor antigen-specific antibody chimeric proteins, have been developed to selectively kill targeted cancer cells. The epidermal growth factor receptor (EGFR) is an attractive target for the development of anti-EGFR ITs against solid tumors due to its overexpression on the cell surface of various solid tumors. However, the low basal level expression of EGFR in normal tissue cells can cause undesirable on-target/off-tumor toxicity and reduce the therapeutic window of anti-EGFR ITs. Here, based on an anti-EGFR monobody with cross-reactivity to both human and murine EGFR, we developed a strategy to tailor the anti-EGFR affinity of the monobody-based ITs carrying a 24-kDa fragment of Pseudomonas exotoxin A (PE24), termed ER-PE24, to distinguish tumors that overexpress EGFR from normal tissues. Five variants of ER-PE24 were generated with different EGFR affinities (KD ≈ 0.24 nM to 104 nM), showing comparable binding activity for both human and murine EGFR. ER/0.2-PE24 with the highest affinity (KD ≈ 0.24 nM) exhibited a narrow therapeutic window of 19 pM to 93 pM, whereas ER/21-PE24 with an intermediate affinity (KD ≈ 21 nM) showed a much broader therapeutic window of 73 pM to 1.5 nM in in vitro cytotoxic assays using tumor model cell lines. In EGFR-overexpressing tumor xenograft mouse models, the maximum tolerated dose (MTD) of intravenous injection of ER/21-PE24 was found to be 0.4 mg/kg, which was fourfold higher than the MTD (0.1 mg/kg) of ER/0.2-PE24. Our study provides a strategy for the development of IT targeting tumor overexpressed antigens with basal expression in broad normal tissues by tailoring tumor antigen affinities.
Reoxygenation Modulates the Adverse Effects of Hypoxia on Wound Repair
Authors: Bai Que , Gao Qian , Hu Fangfang , Zheng Caiyun , Sun Na , Chen Wenting , Liu Jinxi , Zhang Yanni , Wu Xianglong , Lu Tingli
Published: December 2022

Hypoxia is a major stressor and a prominent feature of pathological conditions, such as bacterial infections, inflammation, wounds, and cardiovascular defects. In this study, we investigated whether reoxygenation has a protective effect against hypoxia-induced acute injury and burn using the C57BL/6 mouse model. C57BL/6 mice were exposed to hypoxia and treated with both acute and burn injuries and were in hypoxia until wound healing. Next, C57BL/6 mice were exposed to hypoxia for three days and then transferred to normoxic conditions for reoxygenation until wound healing. Finally, skin wound tissue was collected to analyze healing-related markers, such as inflammation, vascularization, and collagen. Hypoxia significantly increased inflammatory cell infiltration and decreased vascular and collagen production, and reoxygenation notably attenuated hypoxia-induced infiltration of inflammatory cells, upregulation of pro-inflammatory cytokine levels (IL-6 and TNF-α) in the wound, and remission of inflammation in the wound. Immunofluorescence analysis showed that reoxygenation increased the expression of the angiogenic factor α-SMA and decreased ROS expression in burn tissues compared to hypoxia-treated animals. Moreover, further analysis by qPCR showed that reoxygenation could alleviate the expression of hypoxic-induced inflammatory markers (IL-6 and TNF), increase angiogenesis (SMA) and collagen synthesis (Col I), and thus promote wound healing. It is suggested that oxygen can be further evaluated in combination with oxygen-releasing materials as a supplementary therapy for patients with chronic hypoxic wounds.
Protein-Based Oncopanel as Addition to Target Sequencing in Head and Neck Squamous Cell Carcinoma to Individualize Treatment Decisions
Authors: von Witzleben Adrian , Müller-Richter Urs , Maurus Katja , Brändlein Stephanie , Theodoraki Marie-Nicole , Brunner Cornelia , Laban Simon , Lennerz Jochen , Möller Peter , Hoffmann Thomas K. , Doescher Johannes , Schuler Patrick J.
Published: December 2022

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of cancers and patients have limited therapy options if primary treatment fails. Therefore, additional information about the biology of the tumor is essential. Here we performed a feasibility study of concurrently applying two precision diagnostic tools in a consecutive series of HNSCC patients. We analyzed tumor samples of 31 patients using a genomic (oncomine) and a proteomic, immunohistochemical approach (oncopanel) and compared the result, also in the focus on their overlapping therapeutical targets. We found no strong correlation between the two approaches and observed a higher proportion of marker expression for the immunohistochemical panel. However, both panels show in our HNSCC cohort distinct patterns with druggable targets. The data suggest that both approaches complement one another and can be applied side-by-side to identify the best targets for the development of individual treatment options for HNSCC patients.
Combination of Biochemical, Molecular, and Synchrotron-Radiation-Based Techniques to Study the Effects of Silicon in Tomato (Solanum Lycopersicum L.)
Authors: Marmiroli Marta , Mussi Francesca , Gallo Valentina , Gianoncelli Alessandra , Hartley William , Marmiroli Nelson
Published: December 2022

The work focused on the analysis of two cultivars of tomato (Solanum lycopersicum L.), Aragon and Gladis, under two different treatments of silicon, Low, 2 L of 0.1 mM CaSiO3, and High, 0.5 mM CaSiO3, weekly, for 8 weeks, under stress-free conditions. We subsequently analyzed the morphology, chemical composition, and elemental distribution using synchrotron-based µ-XRF techniques, physiological, and molecular aspects of the response of the two cultivars. The scope of the study was to highlight any significant response of the plants to the Si treatments, in comparison with any response to Si of plants under stress. The results demonstrated that the response was mainly cultivar-dependent, also at the level of mitochondrial-dependent oxidative stress, and that it did not differ from the two conditions of treatments. With Si deposited mainly in the cell walls of the cells of fruits, leaves, and roots, the treatments did not elicit many significant changes from the point of view of the total elemental content, the physiological parameters that measured the oxidative stress, and the transcriptomic analyses focalized on genes related to the response to Si. We observed a priming effect of the treatment on the most responsive cultivar, Aragon, in respect to future stress, while in Gladis the Si treatment did not significantly change the measured parameters.
Identification of Iguania Ancestral Syntenic Blocks and Putative Sex Chromosomes in the Veiled Chameleon (Chamaeleo calyptratus, Chamaeleonidae, Iguania)
Authors: Tishakova Katerina V. , Prokopov Dmitry Yu. , Davletshina Guzel I. , Rumyantsev Alexander V. , O’Brien Patricia C. M. , Ferguson-Smith Malcolm A. , Giovannotti Massimo , Lisachov Artem P. , Trifonov Vladimir A.
Published: December 2022

The veiled chameleon (Chamaeleo calyptratus) is a typical member of the family Chamaeleonidae and a promising object for comparative cytogenetics and genomics. The karyotype of C. calyptratus differs from the putative ancestral chameleon karyotype (2n = 36) due to a smaller chromosome number (2n = 24) resulting from multiple chromosome fusions. The homomorphic sex chromosomes of an XX/XY system were described recently using male-specific RADseq markers. However, the chromosomal pair carrying these markers was not identified. Here we obtained chromosome-specific DNA libraries of C. calyptratus by chromosome flow sorting that were assigned by FISH and sequenced. Sequence comparison with three squamate reptiles reference genomes revealed the ancestral syntenic regions in the C. calyptratus chromosomes. We demonstrated that reducing the chromosome number in the C. calyptratus karyotype occurred through two fusions between microchromosomes and four fusions between micro-and macrochromosomes. PCR-assisted mapping of a previously described Y-specific marker indicates that chromosome 5 may be the sex chromosome pair. One of the chromosome 5 conserved synteny blocks shares homology with the ancestral pleurodont X chromosome, assuming parallelism in the evolution of sex chromosomes from two basal Iguania clades (pleurodonts and acrodonts). The comparative chromosome map produced here can serve as the foundation for future genome assembly of chameleons and vertebrate-wide comparative genomic studies.
Conserved Double Translation Initiation Site for Δ160p53 Protein Hints at Isoform’s Key Role in Mammalian Physiology
Authors: López-Iniesta Maria José , Parkar Shrutee N. , Ramalho Ana Catarina , Lacerda Rafaela , Costa Inês F. , Zhao Jingyuan , Romão Luísa , Candeias Marco M.
Published: December 2022

p53 is the most commonly mutated gene in human cancers. Two fundamental reasons for this are its long protein isoforms protect from cancer, while its shorter C-terminal isoforms can support cancer and metastasis. Previously, we have shown that the Δ160p53 protein isoform enhances survival and the invasive character of cancer cells. Here, we identified a translation initiation site nine codons downstream of codon 160—the known initiation codon for the translation of Δ160p53—that is recognized by the translation machinery. When translation failed to initiate from AUG160 due to mutation, it initiated from AUG169 instead, producing similar levels of a similar protein, Δ169p53, which promoted cell survival as efficiently as Δ160p53 following DNA damage. Interestingly, almost all mammalian species with an orthologue to human AUG160 also possess one for AUG169, while none of the non-mammalian species lacking AUG160 have AUG169, even if that region of the p53 gene is well conserved. In view of our findings, we do not believe that Δ169p53 acts as a different p53 protein isoform; instead, we propose that the double translation initiation site strengthens the translation of these products with a critical role in cell homeostasis. Future studies will help verify if this is a more general mechanism for the expression of essential proteins in mammals.