bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative analysis identifies oxidative stress biomarkers in non-alcoholic fatty liver disease via machine learning and weighted gene co-expression network analysis
Authors: Wang Haining , Cheng Wei , Hu Ping , Ling Tao , Hu Chao , Chen Yongzhen , Zheng Yanan , Wang Junqi , Zhao Ting , You Qiang
Published: February 2024

Background

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease globally, with the potential to progress to non-alcoholic steatohepatitis (NASH), cirrhosis, and even hepatocellular carcinoma. Given the absence of effective treatments to halt its progression, novel molecular approaches to the NAFLD diagnosis and treatment are of paramount importance.

Methods

Firstly, we downloaded oxidative stress-related genes from the GeneCards database and retrieved NAFLD-related datasets from the GEO database. Using the Limma R package and WGCNA, we identified differentially expressed genes closely associated with NAFLD. In our study, we identified 31 intersection genes by analyzing the intersection among oxidative stress-related genes, NAFLD-related genes, and genes closely associated with NAFLD as identified through Weighted Gene Co-expression Network Analysis (WGCNA). In a study of 31 intersection genes between NAFLD and Oxidative Stress (OS), we identified three hub genes using three machine learning algorithms: Least Absolute Shrinkage and Selection Operator (LASSO) regression, Support Vector Machine - Recursive Feature Elimination (SVM-RFE), and RandomForest. Subsequently, a nomogram was utilized to predict the incidence of NAFLD. The CIBERSORT algorithm was employed for immune infiltration analysis, single sample Gene Set Enrichment Analysis (ssGSEA) for functional enrichment analysis, and Protein-Protein Interaction (PPI) networks to explore the relationships between the three hub genes and other intersecting genes of NAFLD and OS. The distribution of these three hub genes across six cell clusters was determined using single-cell RNA sequencing. Finally, utilizing relevant data from the Attie Lab Diabetes Database, and liver tissues from NASH mouse model, Western Blot (WB) and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted, this further validated the significant roles of CDKN1B and TFAM in NAFLD.

Results

In the course of this research, we identified 31 genes with a strong association with oxidative stress in NAFLD. Subsequent machine learning analysis and external validation pinpointed two genes: CDKN1B and TFAM, as demonstrating the closest correlation to oxidative stress in NAFLD.

Conclusion

This investigation found two hub genes that hold potential as novel targets for the diagnosis and treatment of NAFLD, thereby offering innovative perspectives for its clinical management.
Effects of estuary reopening management on the fish community in the Nakdong River Estuary
Authors: Hong Donghyun , Gim Jeong-Soo , Joo Gea-Jae , Kim Dong-Kyun , Choi Daehyun , Lee Hak-Young , Jeong Kwang-Seuk , Jo Hyunbin
Published: February 2024

Estuary reopening is a means of restoring estuarine habitats, which has recently been implemented in a few developed countries. The regeneration of a brackish zone in the Nakdong River Estuary (NRE), South Korea, were tested through a series of barrage reopening. During the same period, we conducted extensive fish surveys in the upper part of the NRE barrage on a monthly basis from 2017 to 2021, and subsequently determined whether fish populations and communities were affected by the reopening. The results showed that the reopening of the NRE hardly affected the fish community structure, as non-native species such asErythroculter erythropterusandLepomis macrochirusmaintained their dominance. Still, we discovered that certain euryhaline species are positively affected by estuary reopening, as total 46 Japanese eels (Anguilla japonica) were captured after the reopening, which had not been detected before the reopening. By comparing the size structure of various fish species, we discovered that size distribution of native and migratory species presented more positively skewed pattern after the reopening, while size structure in non-native species remained relatively unchanged normally distributed pattern. Piecewise structural equation modelling revealed that the NRE had become more complex ecosystem, as migratory fish species abundance and biomass started to show a positive correlation with hydraulic factors such as discharge and negative correlation with seasonality after the reopening. We concluded that estuary reopening created some changes in migratory and native freshwater species but such changes were not notably detected in non-native species. Therefore, appropriate sluice operation methodologies, such as considering the migration seasons of migratory species, should be developed. Additionally, human-involved management policies are required to regulate non-native species populations.
Formulation and evaluation of ophthalmic microemulsion for enhanced topical administration of brinzolamide
Authors: Zafar Sehrish , Nazar Muhammad Faizan , Siddique Muhammad Yasir , Haider Sajjad , Alam Kamran , Saleem Muhammad Atif , Shaukat Saadia , Abd Ur Rahman Hafiz Muhammad , Ullah Zaka
Published: February 2024

Microemulsions (μEs) are more effective than conventional formulations for ophthalmic use due to their optical transparency, thermodynamic stability, structural flexibility and higher bioavailability. In addition, μE formulations can increase the water solubility of the drug and improve drug absorption in the eye. Herein, we report the development of three new biocompatible μE formulations containing an antihypertensive drug brinzolamide (BZD) and their evaluation for topical ocular administration. For this, Formulations A, B and C were optimized using an appropriate ratio of isopropyl myristate (IPM) as oil phase, water as aqueous phase and 2-propanol as co-surfactant, while Tween-80, Tween-20 and Tween-60 were selected as surfactant for each formulation, respectively. Preliminary, pseudoternary phase diagrams were delineated and then electrical conductivity and optical microscopy were used to establish optimal formulation for each μE to upheld the appropriate amount of BZD, i.e., 2.0 wt%, 2.0 wt%, and 1.0 wt% in formulation A, B and C, respectively. Dynamic light scattering demonstrated very fine monomodal assembly of BZD-μE nanodroplets (∼50 nm), while FTIR analysis showed effective encapsulation of BZD into hydrophobic microenvironment with no observable chemical interaction between BZD and μE excipients, which was further verified by the peak-to-peak concomitant measurement of fluorescence. Further,in-vitrorelease of BZD-μE showed enhanced and persistent topical ocular administration (>99%) within 10 h demonstrating the appropriate formulation for topical instillation.
Can acupuncture increase microcirculation in peripheral artery disease and diabetic foot syndrome? – a pilot study
Authors: Valentini Jan , Sigl Martin , Dunckel Cornelia , Krisam Johannes , Amendt Klaus , Greten Henry Johannes
Published: February 2024

Background

Globally, diabetes mellitus (DM) and peripheral artery disease (PAD) have an increasing incidence and a high prevalence and are both associated with high morbidity and complication rates, e.g., as chronic non-healing peripheral ulcers. Impaired macro- and microcirculation and peripheral neuropathy lead to an increased risk of foot ulcers and infections. These complications are difficult to treat, have a high risk of becoming chronic and often lead to lower limb amputation. The aim of this planned study was to investigate the potential effects of acupuncture on improving microcirculation in patients with Diabetic Foot Syndrome (DFS) and PAD.

Materials and methods

In 18 patients with chronic non-healing peripheral ulcers and diagnosed DM or PAD, data on 8 microcirculatory parameters were collected simultaneously on intact skin close to the wound margin. Microcirculation was assessed using an O2C device combining laser Doppler shift and white light spectroscopy (LEA Medizintechnik GmbH, Giessen, Germany). Unilateral and bilateral acupuncture was performed on the connecting line between acupuncture points Stomach 14 and Stomach 15.

Results

After unilateral acupuncture (ipsilateral to the wound side), a statistically significant improvement in 7 out of 8 microcirculatory parameters was demonstrated compared to baseline measurements before acupuncture. After bilateral acupuncture, there was an additional improvement and statistical significance in all parameters in both DFS and PAD patients.

Discussion

These results show an improvement in the microcirculation and peripheral blood flow at the edges of the wound. As impaired micro- and macrocirculation is considered to be a critical prognostic factor for the healing of a peripheral lesion, the intervention could have a positive impact on the healing of (chronic) peripheral wounds.
Anti-diabetic effect of red quinoa polysaccharide on type 2 diabetic mellitus mice induced by streptozotocin and high-fat diet
Authors: Zang Yanqing , Ge Yinchen , Cao Yang , Tang Huacheng
Published: February 2024

The purpose of this study was to explore the mechanism of red quinoa polysaccharide (RQP) in alleviating type 2 diabetes (T2D) through in vivo and in vitro experiments. Results of HPLC and FITR showed that RQP was a complex polysaccharide and contained more glucose, galactose and acarbose. In vitro experiments, RQP showed strong antioxidant capacity and inhibition on α-amylase and α-glucosidase. In vivo experiments, RQP was proved to induce a significant improvement of diabetes after 4 weeks of ingestion, including the abilities of lowering blood glucose, regulating lipid metabolism, anti-oxidation and promoting secretion of SCFAs. Furthermore, 16S rRNA study demonstrated that RQP transformed the intestinal microbiota composition in diabetic mice, decreased the abundance of norank_f_Muribaculaceae and Lachnospiraceae_NK4A136_group, and increased the relative abundance of Akkermansia, unclassified_f_Lachnospiraceae, norank_f_Eubacterium_coprostanoligenes_group, unclassified_f_Atopobiaceae and norank_f_Lachnospiraceae. The biosynthetic pathways, metabolic pathways and intestinal microbiome phenotypes in mice also changed accordingly. In conclusion, this study suggests that RQP can inhibit the development of diabetes by correcting the imbalance of intestinal flora.
Exploring the complex interplay: gut microbiome, stress, and leptospirosis
Authors: Petakh Pavlo , Oksenych Valentyn , Kamyshna Iryna , Boisak Iryna , Lyubomirskaya Katerina , Kamyshnyi Oleksandr
Published: February 2024

Leptospirosis, a re-emerging zoonotic disease, remains a significant global health concern, especially amid floods and disasters such as the Kakhovka Dam destruction. As is known, the stress that occurs in the conditions of military conflicts among civilian and military personnel significantly affects susceptibility to infectious diseases and possibly even influences their course. This review aims to explore how the gut microbiome and stress mediators (such as catecholamines and corticosteroids) might impact the leptospirosis disease course. The review opens new horizons for research by elucidating the connections between the gut microbiome, stress, and leptospirosis.
Dual-task turn velocity – a novel digital biomarker for mild cognitive impairment and dementia
Authors: Wang Jing , Zhou Zheping , Cheng Shanshan , Zhou Li , Sun Xiaoou , Song Ziyang , Wu Zhiwei , Lu Jinhua , Qin Yiren , Wang Yueju
Published: February 2024

Background

Disorders associated with cognitive impairment impose a significant burden on both families and society. Previous studies have indicated that gait characteristics under dual-task as reliable markers of early cognitive impairment. Therefore, digital gait detection has great potential for future cognitive screening. However, research on digital biomarkers based on smart devices to identify cognitive impairment remains limited. The aim of this study is to explore digital gait biomarkers by utilizing intelligent wearable devices for discriminating mild cognitive impairment and dementia.

Methods

This study included 122 subjects (age: 74.7 ± 7.7 years) diagnosed with normal cognition (NC, n = 38), mild cognitive impairment (MCI, n = 42), or dementia (n = 42). All subjects underwent comprehensive neuropsychological assessments and cranial Magnetic Resonance Imaging (MRI). Gait parameters were collected using validated wearable devices in both single-task and dual-task (DT). We analyzed the ability of gait variables to predict MCI and dementia, and examined the correlations between specific DT-gait parameters and sub-cognitive functions as well as hippocampal atrophy.

Results

Our results demonstrated that dual-task could significantly improve the ability to predict cognitive impairment based on gait parameters such as gait speed (GS) and stride length (SL). Additionally, we discovered that turn velocity (TV and DT-TV) can be a valuable novel digital marker for predicting MCI and dementia, for identifying MCI (DT-TV: AUC = 0.801, sensitivity 0.738, specificity 0.842), and dementia (DT-TV: AUC = 0.923, sensitivity 0.857, specificity 0.842). The correlation analysis and linear regression analysis revealed a robust association between DT-TV and memory function, as well as the hippocampus atrophy.

Conclusion

This study presents a novel finding that DT-TV could accurately identify varying degrees of cognitive impairment. DT-TV is strongly correlated with memory function and hippocampus shrinkage, suggests that it can accurately reflect changes in cognitive function. Therefore, DT-TV could serve as a novel and effective digital biomarker for discriminating cognitive impairment.
Modeling sporadic Alzheimer’s disease in mice by combining Apolipoprotein E4 risk gene with environmental risk factors
Authors: Ganesan Kiruthika , Rentsch Peggy , Langdon Alexander , Milham Luke T. , Vissel Bryce
Published: February 2024

Introduction

Developing effective treatment for Alzheimer’s disease (AD) remains a challenge. This can be partially attributed to the fact that the mouse models used in preclinical research largely replicate familial form of AD, while majority of human cases are sporadic; both forms differ widely in the onset and origin of pathology, therefore requiring specific/targeted treatments.

Methods

In this study, we aimed to model sporadic AD in mice by combining two of the many risk factors that are strongly implicated in AD: ApoE4, a major genetic risk factor, together with an inflammatory stimuli. Accordingly, we subjected ApoE4 knock in (KI) mice, expressing humanized ApoE4, to low doses of Lipopolysaccharide (LPS) injections (i.p, weekly, for 4 months).

Results

We assessed these animals for behavioral impairments at 6 months of age using Open Field, Y-maze, and Barnes Maze Test. LPS induced hypoactivity was observed in the Open Field and Y-maze test, whereas spatial learning and memory was intact. We then quantified differences in dendritic spine density, which is a strong correlate of AD. ApoE4KI mice showed a significant reduction in the number of spines after treatment with LPS, whereas there were no obvious differences in the total number of microglia and astrocytes.

Discussion

To conclude, in the current study the APoEe4 risk gene increases the vulnerability of hippocampal neurons to inflammation induced spine loss, laying a foundation for an early sporadic AD mouse model.
The ventral hippocampus is activated in olfactory but not auditory threat memory
Authors: Sepahvand Tayebeh , Carew Samantha J. , Yuan Qi
Published: February 2024

Hippocampal networks required for associative memory formation are involved in cue- and context-dependent threat conditioning. The hippocampus is functionally heterogeneous at its dorsal and ventral poles, and recent investigations have focused on the specific roles required from each sub-region for associative conditioning. Cumulative evidence suggests that contextual and emotional information is processed by the dorsal and ventral hippocampus, respectively. However, it is not well understood how these two divisions engage in threat conditioning with cues of different sensory modalities. Here, we compare the involvement of the dorsal and ventral hippocampus in two types of threat conditioning: olfactory and auditory. Our results suggest that the dorsal hippocampus encodes contextual information and is activated upon recall of an olfactory threat memory only if contextual cues are relevant to the threat. Overnight habituation to the context eliminates dorsal hippocampal activation, implying that this area does not directly support cue-dependent threat conditioning. The ventral hippocampus is activated upon recall of olfactory, but not auditory, threat memory regardless of habituation duration. Concurrent activation of the piriform cortex is consistent with its direct connection with the ventral hippocampus. Together, our study suggests a unique role of the ventral hippocampus in olfactory threat conditioning.
Association between Sjögren syndrome, sociodemographic factors, comorbid conditions, and optic neuritis: a Taiwanese population-based study
Authors: Jan Ren-Long , Ho Chung-Han , Sung Cheng-Hao , Wang Jhi-Joung , Jan Han-Yi , Chen Wei-Yu , Chang Yuh-Shin
Published: February 2024

Purpose

Our study aimed to explore the correlation between Sjögren syndrome, sociodemographic factors, comorbid conditions, and optic neuritis.

Methods

This retrospective, nationwide, population-based, matched case–control investigation involved 33,190 individuals diagnosed with optic neuritis, identified using the International Classification of Diseases, Ninth Revision, Clinical Modification codes 377.30 for optic neuritis or 377.32 for retrobulbar neuritis. Patient data were extracted from the Taiwan National Health Insurance Research Database. Demographic characteristics, the presence of Sjögren syndrome, and pre-existing comorbid conditions were analyzed using univariate logistic regression. Continuous variables were assessed with a paired t-test. Adjusted logistic regression was employed to compare the prognosis odds ratio (OR) of patients with optic neuritis to controls.

Results

After adjusting for confounding variables, individuals with Sjögren syndrome exhibited a significantly higher likelihood of developing optic neuritis compared to controls (adjusted OR, 9.79; 95% confidence interval [CI], 7.28–12.98; p < 0.0001). Other conditions associated with increased odds of optic neuritis included rheumatoid arthritis, ankylosing spondylitis, multiple sclerosis, systemic lupus erythematosus, and granulomatous vasculitis (adjusted OR: 1.57, 95% CI: 1.33–1.86; adjusted OR: 2.02, 95% CI: 1.65–2.48; adjusted OR: 140.77, 95% CI: 35.02–565.85; adjusted OR: 2.38, 95% CI: 1.71–3.30; adjusted OR: 18.28, 95% CI: 2.21–151.45, respectively), as well as systemic infections such as human herpes viral infection and tuberculosis infection (adjusted OR: 1.50, 95% CI: 1.35–1.66; adjusted OR: 4.60, 95% CI: 3.81–5.56, respectively).

Discussion

Our findings strongly support the existence of an association between Sjögren syndrome, rheumatoid arthritis, ankylosing spondylitis, multiple sclerosis, systemic lupus erythematosus, granulomatous vasculitis, human herpes viral infection, tuberculosis, and optic neuritis.
Machine learning-based diagnostic model for preoperative differentiation between xanthogranulomatous cholecystitis and gallbladder carcinoma: a multicenter retrospective cohort study
Authors: Fu Tianwei , Bao Yating , Zhong Zhihan , Gao Zhenyu , Ye Taiwei , Zhang Chengwu , Jing Huang , Xiao Zunqiang
Published: February 2024

Background

Xanthogranulomatous cholecystitis (XGC) and gallbladder carcinoma (GBC) share similar imaging and serological profiles, posing significant challenges in accurate preoperative diagnosis. This study aimed to identify reliable indicators and develop a predictive model to differentiate between XGC and GBC.

Methods

This retrospective study involved 436 patients from Zhejiang Provincial People’s Hospital and The Affiliated Lihuili Hospital of Ningbo University. Comprehensive preoperative imaging, including ultrasound, Computed Tomography (CT), Magnetic Resonance Imaging (MRI), and blood tests, were analyzed. Machine learning (Random Forest method) was employed for variable selection, and a multivariate logistic regression analysis was used to construct a nomogram for predicting GBC. Statistical analyses were performed using SPSS and RStudio software.

Results

The study identified gender, Murphy’s sign, absolute neutrophil count, glutamyl transpeptidase level, carcinoembryonic antigen level, and comprehensive imaging diagnosis as potential risk factors for GBC. A nomogram incorporating these factors demonstrated high predictive accuracy for GBC, outperforming individual or combined traditional diagnostic methods. External validation of the nomogram showed consistent results.

Conclusion

The study successfully developed a predictive nomogram for distinguishing GBC from XGC with high accuracy. This model, integrating multiple clinical and imaging indicators, offers a valuable tool for clinicians in making informed diagnostic decisions. The findings advocate for the use of comprehensive preoperative evaluations combined with advanced analytical tools to improve diagnostic accuracy in complex medical conditions.
Psychological/behavioral interventions for emerging adults with chronic pain
Authors: Foy Judith G. , Kechichian Sandra , Foy Michael R. , Ziadni Maisa
Published: February 2024

Background

Emerging adults, of whom significant numbers report chronic pain, are characterized as having unique needs and challenges. Psychological/behavioral treatments found to be beneficial for reducing pain outcomes in children and adults are understudied in emerging adults. Following a systematic review of the literature, our objective is to report on quantitative studies of psychological/behavioral interventions for chronic pain in emerging adults.

Method

We conducted a search of six databases (Cochrane Central Register of Controlled Trials, Google Scholar, ProQuest, PsycINFO, PubMed, and Web of Science) and reference sections in dissertations and systematic reviews to 4/29/2023. Keywords and phrases were search term combinations of “chronic/persistent pain”, “emerging/young adults,” and “intervention/treatment” using Boolean logic.

Results

Our review resulted in identifying 37 articles, of which 2 duplicates were removed, and 31 were further excluded by a screening process based on various inclusionary and exclusionary criteria. The search yielded four studies on psychological/behavioral interventions (yoga, acceptance and commitment therapy and relaxation), all of which positively affected the pain experience and/or pain-related outcomes. These studies presented issues in design such as not being blinded or randomized, having a small sample size, and potential confounds that were not reported or examined.

Discussion

The low number of studies reveals a large gap in the literature and is a call-to-action to further expand our understanding of effective and safer psychological/behavioral therapies for chronic pain in emerging adults. Successful pain management during this developmental phase may help young adults achieve positive trajectories for personal, occupational, relational, and health aspects of their lives.
Non-coding RNAs: targets for Chinese herbal medicine in treating myocardial fibrosis
Authors: Wang Minghui , Yan Maocai , Tan Liqiang , Zhao Xiaona , Liu Guoqing , Zhang Zejin , Zhang Jing , Gao Honggang , Qin Wei
Published: February 2024

Cardiovascular diseases have become the leading cause of death in urban and rural areas. Myocardial fibrosis is a common pathological manifestation at the adaptive and repair stage of cardiovascular diseases, easily predisposing to cardiac death. Non-coding RNAs (ncRNAs), RNA molecules with no coding potential, can regulate gene expression in the occurrence and development of myocardial fibrosis. Recent studies have suggested that Chinese herbal medicine can relieve myocardial fibrosis through targeting various ncRNAs, mainly including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs). Thus, ncRNAs are novel drug targets for Chinese herbal medicine. Herein, we summarized the current understanding of ncRNAs in the pathogenesis of myocardial fibrosis, and highlighted the contribution of ncRNAs to the therapeutic effect of Chinese herbal medicine on myocardial fibrosis. Further, we discussed the future directions regarding the potential applications of ncRNA-based drug screening platform to screen drugs for myocardial fibrosis.
Melatonin derivative 6a as a PARP-1 inhibitor for the treatment of Parkinson’s disease
Authors: Ma Qing-Wei , Han Rui-Ting , Wu Zi-Jie , Zhou Jun-Jie , Chen Meng-Ting , Zhang Xiang-Zhi , Ma Wen-Zhe , Feng Na
Published: February 2024

Both continuous oxidative stress and poly (ADP-ribose) polymerase 1 (PARP-1) activation occur in neurodegenerative diseases such as Parkinson’s disease. PARP-1 inhibition can reverse mitochondrial damage and has a neuroprotective effect. In a previous study, we synthesized melatonin derivative 6a (MD6a) and reported that it has excellent antioxidant activity and significantly reduces α-synuclein aggregation in Caenorhabditis elegans; however, the underlying mechanism is largely unknown. In the present study, we revealed that MD6a is a potential PARP-1 inhibitor, leading to mammalian targe of rapamycin/heat shock factor 1 signaling downregulation and reducing heat shock protein 4 and 6 expression, thus helping to maintain protein homeostasis and improve mitochondrial function. Together, these findings suggest that MD6a might be a viable candidate for the prevention and treatment of Parkinson’s disease.
Microglial reactivity in brainstem chemosensory nuclei in response to hypercapnia
Authors: Eugenín Jaime , Beltrán-Castillo Sebastián , Irribarra Estefanía , Pulgar-Sepúlveda Raúl , Abarca Nicolás , von Bernhardi Rommy
Published: February 2024

Microglia, the resident immune cells of the CNS, surveil, detect, and respond to various extracellular signals. Depending on the nature of these signals, an integrative microglial response can be triggered, resulting in a phenotypic transformation. Here, we evaluate whether hypercapnia modifies microglia phenotype in brainstem respiratory-related nuclei. Adult C57BL/6 inbred mice were exposed to 10% CO2 enriched air (hypercapnia), or pure air (control), for 10 or 30 min and immediately processed for immunohistochemistry to detect the ubiquitous microglia marker, ionized calcium binding adaptor molecule 1 (Iba1). Hypercapnia for thirty, but not 10 min reduced the Iba1 labeling percent coverage in the ventral respiratory column (VRC), raphe nucleus (RN), and nucleus tractus solitarius (NTS) and the number of primary branches in VRC. The morphological changes persisted, at least, for 60 min breathing air after the hypercapnic challenge. No significant changes were observed in Iba1+ cells in the spinal trigeminal nucleus (Sp5) and the hippocampus. In CF-1 outbred mice, 10% CO2 followed by 60 min of breathing air, resulted in the reduction of Iba1 labeling percent coverage and the number and length of primary branches in VRC, RN, and NTS. No morphological change was observed in Iba1+ cells in Sp5 and hippocampus. Double immunofluorescence revealed that prolonged hypercapnia increased the expression of CD86, an inflammatory marker for reactive state microglia, in Iba1+ cells in VRC, RN, and NTS, but not in Sp5 and hippocampus in CF-1 mice. By contrast, the expression of CD206, a marker of regulatory state microglia, persisted unmodified. In brainstem, but not in hippocampal microglia cultures, hypercapnia increased the level of IL1β, but not that of TGFβ measured by ELISA. Our results show that microglia from respiratory-related chemosensory nuclei, are reactive to prolonged hypercapnia acquiring an inflammatory-like phenotype.
GmGLU1 and GmRR4 contribute to iron deficiency tolerance in soybean
Authors: Kohlhase Daniel R. , O’Rourke Jamie A. , Graham Michelle A.
Published: February 2024

Iron deficiency chlorosis (IDC) is a form of abiotic stress that negatively impacts soybean yield. In a previous study, we demonstrated that the historical IDC quantitative trait locus (QTL) on soybean chromosome Gm03 was composed of four distinct linkage blocks, each containing candidate genes for IDC tolerance. Here, we take advantage of virus-induced gene silencing (VIGS) to validate the function of three high-priority candidate genes, each corresponding to a different linkage block in the Gm03 IDC QTL. We built three single-gene constructs to target GmGLU1 (GLUTAMATE SYNTHASE 1, Glyma.03G128300), GmRR4 (RESPONSE REGULATOR 4, Glyma.03G130000), and GmbHLH38 (beta Helix Loop Helix 38, Glyma.03G130400 and Glyma.03G130600). Given the polygenic nature of the iron stress tolerance trait, we also silenced the genes in combination. We built two constructs targeting GmRR4+GmGLU1 and GmbHLH38+GmGLU1. All constructs were tested on the iron-efficient soybean genotype Clark grown in iron-sufficient conditions. We observed significant decreases in soil plant analysis development (SPAD) measurements using the GmGLU1 construct and both double constructs, with potential additive effects in the GmRR4+GmGLU1 construct. Whole genome expression analyses (RNA-seq) revealed a wide range of affected processes including known iron stress responses, defense and hormone signaling, photosynthesis, and cell wall structure. These findings highlight the importance of GmGLU1 in soybean iron stress responses and provide evidence that IDC is truly a polygenic trait, with multiple genes within the QTL contributing to IDC tolerance. Finally, we conducted BLAST analyses to demonstrate that the Gm03 IDC QTL is syntenic across a broad range of plant species.
Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane
Authors: Qin Ying , Khan Qaisar , Yan Jia-Wei , Wang Yu-Yi , Pan Yang-Fei , Huang Ying , Wei Jiang-Lu , Guo Dao-Jun , Li Yang-Rui , Dong Deng-Feng , Xing Yong-Xiu
Published: February 2024

Introduction

Sugarcane endophytic nitrogen-fixing bacterium Klebsiella variícola DX120E displayed broad impact on growth, but the exact biological mechanism, especially polyamines (PAs) role, is still meager.

Methods

To reveal this relationship, the content of polyamine oxidase (PAO), PAs, reactive oxygen species (ROS)-scavenging antioxidative enzymes, phytohormones, 1-aminocyclopropane-1-carboxylic synthase (ACS), chlorophyll content, and biomass were determined in sugarcane incubated with the DX120E strain. In addition, expression levels of the genes associated with polyamine metabolism were measured by transcriptomic analysis.

Results

Genomic analysis of Klebsiella variícola DX120E revealed that 39 genes were involved in polyamine metabolism, transport, and the strain secrete PAs in vitro. Following a 7-day inoculation period, DX120E stimulated an increase in the polyamine oxidase (PAO) enzyme in sugarcane leaves, however, the overall PAs content was reduced. At 15 days, the levels of PAs, ROS-scavenging antioxidative enzymes, and phytohormones showed an upward trend, especially spermidine (Spd), putrescine (Put), catalase (CAT), auxin (IAA), gibberellin (GA), and ACS showed a significant up-regulation. The GO and KEGG enrichment analysis found a total of 73 differentially expressed genes, involving in the cell wall (9), stimulus response (13), peroxidase activity (33), hormone (14) and polyamine metabolism (4).

Discussion

This study demonstrated that endophytic nitrogen-fixing bacteria stimulated polyamine metabolism and phytohormones production in sugarcane plant tissues, resulting in enhanced growth. Dual RNA-seq analyses provided insight into the early-stage interaction between sugarcane seedlings and endophytic bacteria at the transcriptional level. It showed how diverse metabolic processes selectively use distinct molecules to complete the cell functions under present circumstances.
Predicting pragmatic functions of Chinese echo questions using prosody: evidence from acoustic analysis and data modeling
Authors: Cao Siyi , Xu Yizhong , Zhou Tongquan , Wu Anqi
Published: February 2024

Echo questions serve two pragmatic functions (recapitulatory and explicatory) and are subdivided into two types (yes-no echo question and wh-echo question) in verbal communication. Yet to date, most relevant studies have been conducted in European languages like English and Spanish. It remains unknown whether the different functions of echo questions can be conveyed via prosody in spoken Chinese. Additionally, no comparison was made on the diversified algorithmic models in predicting functions by the prosodity of Chinese echo questions, a novel linguistic cognition in nature. This motivated us to use different acoustic cues to predict different pragmatic functions of Chinese echo questions by virtue of acoustic experiment and data modeling. The results showed that for yes-no echo question, explicatory function exhibited higher pitch and intensity patterns than recapitulatory function whereas for wh-echo question, recapitulatory function demonstrated higher pitch and intensity patterns than explicatory function. With regard to data modeling, the algorithm Support Vector Machine (SVM) relative to Random Forest (RF) and Logistic Regression (LR) performed better when predicting different functions using prosodic cues in both yes-no and wh-echo questions. This study from a digitized perspective adds evidence to the cognition of echo questions’ functions on a prosodic basis.
Refocusing on nature: the role of nature reintegration in environmental, mental, and societal wellbeing
Authors: Willis Royce L. , Brymer Eric , Sharma-Brymer Vinathe , Leach Matthew
Published: February 2024

This perspective paper investigates the dynamic interplay between wealth, materialism, environmental degradation, and mental health amid escalating challenges of climate change. The paper critically examines how affluence, often a buffer against climate impacts, paradoxically leads to higher consumption and carbon footprints, exacerbating environmental problems. A societal emphasis on materialism contributes to an estrangement from nature, with significant implications for mental health and environmental sustainability. The paper proposes a fundamental shift in addressing these intertwined challenges through reintegration with nature. The paper recommends integrating urban planning, education, mental health, and community engagement strategies to build a sustainable, mentally resilient society more integrated with nature. This approach, supported by future research directions, aims to create a more balanced, environmentally conscious, and mentally healthy world.
The formation of episodic autobiographical memory is predicted by mental imagery, self-reference, and anticipated details
Authors: Lenormand Diane , Fauvel Baptiste , Piolino Pascale
Published: February 2024

Introduction

Despite the ecological nature of episodic memory (EM) and the importance of consolidation in its functioning, studies tackling both subjects are still scarce. Therefore, the present study aims at establishing predictions of the future of newly encoded information in EM in an ecological paradigm.

Methods

Participants recorded two personal events per day with a SenseCam portable camera, for 10 days, and characterized the events with different subjective scales (emotional valence and intensity, self-concept and self-relevance, perspective and anticipated details at a month, mental images…). They then performed a surprise free recall at 5 days and 1 month after encoding. Machine learning algorithms were used to predict the future of events (episodic or forgotten) in memory at 1 month.

Results

The best algorithm showed an accuracy of 78%, suggesting that such a prediction is reliably possible. Variables that best differentiated between episodic and forgotten memories at 1 month were mental imagery, self-reference, and prospection (anticipated details) at encoding and the first free recall.

Discussion

These results may establish the basis for the development of episodic autobiographical memory during daily experiences.
Observation of human-animal interaction for research (OHAIRE) behavior coding in a randomized control trial of children with attention-deficit hyperactivity disorder (ADHD) and a canine-assisted intervention
Authors: Nieforth Leanne O. , Guerin Noémie A. , Stehli Annamarie , Schuck Sabrina E. B. , Yi Katherine , O’Haire Marguerite E.
Published: February 2024

Introduction

Diagnosed in about 10% of children in the United States, attention-deficit hyperactivity disorder (ADHD) is characterized by symptoms including inattention, hyperactivity, and impulsivity. Traditional interventions, such as pharmacological and psychological interventions, are often used in conjunction with integrative health options, such as animal-assisted interventions. The objective of this manuscript is to report behavior coding findings from a randomized control trial of children with ADHD.

Methods

As part of a larger randomized control trial focused on the efficacy of combining a canine-assisted intervention (live therapy dog or control stuffed dog) with cognitive behavioral therapy for children with ADHD, the current manuscript focuses on video-captured behavior observations (n = 35 children, approximately 322 minutes of data). Data were extracted and coded using the Observation of Human-Animal Interaction Research (OHAIRE) Coding System. Behavior codes are reported as summary scores for the following domains: animal social interaction and human social interaction (further separated into human-adult social interaction and human-peer social interaction). Repeated measures mixed models analyses were performed using SAS PROC GLIMMIX to evaluate group differences and change across the study period.

Results

There were no significant differences in how much children interacted with the live therapy dogs versus control stuffed dogs. With respect to human-to-human social interactions, children showed greater increases over time in human-directed social interactions in the presence of live therapy dogs compared to stuffed dogs (p = .020). Over the course of the 12-week intervention, children increased in interactions with both adults (p = .006) and their peers (p = .014); however, there were more increases over time in adult-directed social interactions in the live animal condition compared to the control stuffed animal condition (p < 0.0001).

Discussion & conclusions

Findings suggest changes in social interaction when participating in this canine-assisted intervention, specifically greater increases in human-to-human social interactions over time when a live therapy dog is present compared to a control stuffed dog. Children appear to engage relatively equally with both live and stuffed dogs; however, the impact of animals on human socialization differs based on if a live animal is present. Future studies should consider incorporating behavior coding analysis into studies of canine-assisted interventions to identify how human-animal interactions may be moderators or mechanisms for psychosocial outcomes.
The impact of public transportation on the transmission of COVID-19 in Rwanda
Authors: Umutoni Brigitte , Tumushime Jean Claude , Hewins Benjamin , Udahemuka Jean Claude , Ndishimye Pacifique , Kelvin David J. , Sganzerla Martinez Gustavo
Published: February 2024

Introduction

The onset of the COVID-19 pandemic has placed a significant burden on healthcare systems worldwide, particularly in sub-Saharan regions where healthcare resources are limited. The transmission of SARS-CoV-2 is facilitated by the movement of people from place to place. Therefore, implementing measures that restrict movement of people and contacts is crucial in controlling the spread of the disease. Following the identification of the first COVID-19 case in Rwanda, the government implemented stringent measures, including a complete nationwide lockdown, border closures, curfews, reduced capacity in public transportation and businesses, and mandatory testing. This study aims to assess epidemiological trends in COVID-19 cases in relation to changes in population mobility within the public transportation system.

Methods

A descriptive analysis using publicly available data on COVID-19 epidemiological indicators (cases, deaths, vaccinations, and stringency index) and mobility data was conducted.

Results

The results reveal a strong correlation between mobility in public transportation and other activities, underscoring Rwanda’s reliance on its public transportation system. The study also identifies a pattern where increases in transit station mobility preceded spikes in COVID-19 cases, suggesting that the subsequent rise in public transportation usage may contribute to higher infection rates.

Discussion

Therefore, this study emphasizes the importance of ongoing vigilance and regulatory measures regarding public transportation during infectious disease outbreaks.
Stress events and stress symptoms in Chinese secondary school students: gender and academic year characteristics of the relationship
Authors: Ma Aimin , Tan Shuying , Chen Jin , Lou Hu
Published: February 2024

Objective

To explore the relationship between stress events and stress symptoms and their gender and academic year characteristics in Chinese secondary school students.

Methods

4,995 secondary school students were investigated by the Adolescent Self-rating Life Events Checklist (ASLEC) and the Calgary Symptoms of Stress Inventory (C-SOSI).

Results

First, there were significant differences in all dimensions and total scores of stress events and stress symptoms between boys and girls in secondary school and between junior high school students and senior high school students. Second, the dimensions and total scores of stress events in secondary school students are positively correlated with the dimensions and total scores of stress symptoms. Third, the influence of punishment on the stress symptoms of secondary school boys is the most obvious, and the influence of punishment, adaption, relationship stress, and learning stress on the stress symptoms of secondary school girls is the most obvious. The influence of punishment on the stress symptoms of junior high school students is the most obvious, and the influence of punishment and relationship on the stress symptoms of senior high school students is the most obvious.

Conclusion

Stress events and stress symptoms of Chinese secondary school students have significant differences in gender and academic year. The same stress event has different influence mechanisms on the stress symptoms of Chinese secondary school students of different genders and different academic years.
Meaning making and fostering radical hope: applying positive psychology to eco-anxiety research in youth
Authors: Malboeuf-Hurtubise Catherine , Léger-Goodes Terra , Herba Catherine M. , Bélanger Nadia , Smith Jonathan , Marks Elizabeth
Published: February 2024

The consequences of human activity on climate change are increasingly apparent. For example, they are causing ecological degradation and affecting human and animal health. Rightly so, it is considered as the most important challenge of this century. Researchers in psychology and mental health developed an interest in the direct and indirect effects of climate and ecological change on people's psychological wellbeing, which is referred to as a concept described as eco-anxiety or eco-distress. It is worth emphasizing that climate issues are taking a larger place in the school curriculum for youth in elementary, middle and high schools. Youth are thus increasingly aware of the major threat and understandably report legitimate concerns and worries. For some youth, eco-anxiety leads to greater involvement and activism, as can be seen by the international movement set out and led by youth activist Greta Thunberg. However, eco-anxiety can also lead to feelings of hopelessness and disengagement. Despite contributing the least to the climate and ecological crises, youth will be most affected by the impacts, and will carry the burden of the climate crisis throughout their lives. Researchers, educators and mental health professionals must therefore find ways to foster youth psychosocial wellbeing and resilience alongside ensuring that their voices are heard. To this end, it is vital that young people feel able to openly discuss climate change and associated issues alongside the distressing thoughts and feelings they engender. This can be supported by using various psychological approaches to develop effective interventions. Researchers and clinicians in child mental health could gain from drawing from research in positive psychology to develop such interventions. In this review and commentary, we will outline how eco-anxiety and child psychological wellbeing can be framed within a positive psychology framework, including the relevance of self-determined motivation. Insights from interventions based on positive psychology including exercises to foster hope, forgiveness and meaning making will also be discussed. We will highlight how such interventions can be adapted as powerful tools to foster child wellbeing and cope with their eco-anxiety.
Assessing and mapping water-energy-food nexus smart innovations and practices in Vhembe District Municipality, Limpopo Province, South Africa
Authors: Musetsho Khangwelo D. , Mwendera Emmanuel , Madzivhandila Tshilidzi , Makungo Rachel , Volenzo Tom E. , Mamphweli Ntshengedzeni S. , Nephawe Khathutshelo A.
Published: February 2024

Water, energy, and food and their interactions (commonly referred to as the WEF nexus) are critical pillars to resolving the intractable global challenges such as poverty, hunger, malnutrition, poor sanitation, climate, and health crises. The nexus approach, practices, and innovations at the household level are critical determinants of whether resource use efficiency, co-benefits, basic rights to water and food, and sustainability governance are attained. In particular, smart WEF innovations can contribute to the current generations' economic, social, and environmental needs without compromising the needs of the future generation. The study aimed to identify smart innovations, practices, and factors influencing their adoption to inform policy and decision-making processes. The study intends to support scaling up the adoption of innovations and practices that enhance sustainability and resource security in support of the sustainable development goals (SDGs). Semi-structured interviews and key informant interviews (KII) supplemented with observational checklists were used to identify the WEF nexus smart technologies, innovations, and practices in Vhembe District Municipality, Limpopo Province, South Africa. Data were collected from a sample size of 128 households in the study area. Our findings revealed synergistic smart innovation practices across WEF resource use and management practices. Though indigenous knowledge (IK) practices were widely evident in the study area, non-existent WEF smart knowledge support systems existed in the study area. Indigenous knowledge practices were the most elicited innovation by 99.2% of households, suggesting it is critical to advancing WEF smart innovations and practices and needs to be integrated into any policy and governance interventions. A proportion of households recycle water (27%), whilst 53% use untreated water. Furthermore, the knowledge systems on smart WEF innovations were fragmented despite their potential to synergize sustainability objectives. Exploring innovation platforms (IPs) as vehicles for dissemination, innovation, and extension and advisory service delivery, as well as validation of Indigenous Knowledge Systems (IKS), has the potential to contribute to the diffusion, uptake, and scaling of existing innovation and practices with significant spill-over effects on WEF resource security and sustainability outcomes both at local and extra local scales.