bluuebunny/crossref_metadata_2025 huggingface dataset:
A preliminary association study of fat mass and obesity associated gene polymorphisms and degenerative disc disease in a Chinese Han population
Authors: Lao Lifeng , Zhong Guibin , Li Xinfeng , Liu Zude
Published: December 2013

Objective

To assess whether polymorphisms of the fat mass and obesity associated gene ( FTO) are associated with the presence and severity of degenerative disc disease (DDD) in a Chinese Han population.

Methods

In this case–control study, patients with DDD and controls matched for age, sex and body mass index were genotyped for six single nucleotide polymorphisms (SNPs) of FTO. Disease severity was measured using the Japanese Orthopaedic Association score. Allelic, genotypic and genotype–phenotype association analyses were performed.

Results

A total of 80 patients with DDD and 80 controls were studied. All six SNPs were in Hardy–Weinberg equilibrium. The frequencies of allele G and genotype G/G of the SNP rs11076008 were significantly associated with DDD after Bonferroni correction. No associations were shown between the SNPs studied and sex or disease severity.

Conclusion

The SNP rs11076008 of FTO may play an important role in the development of DDD in a Chinese Han population. The G/G genotype and/or G allele may be a risk factor for DDD. These results suggest that FTO is a DDD predisposition gene and may support a close relationship between obesity and DDD.
The role of in vivo confocal microscopy in the diagnosis of hidden corneal foreign bodies
Authors: Xu Zhongzhong , Yu Xiaofei , Li Zhijie , Wang Liya
Published: December 2013

Objective

To investigate in vivo confocal microscopy (IVCM) to diagnose hidden corneal foreign bodies.

Methods

Male Kunming mice ( n = 25; 12 weeks old) were anaesthetized prior to the insertion of five different materials (spiny wood, rusty iron, sharp stone, sharp glass fragment and human hair fragment) into the cornea by different traumatic processes. A separate mouse was used for each corneal foreign body. The corneas of the mice were scanned 24 h later by a laser scanning IVCM to establish the characteristics (shape, reflectivity and depth in the cornea) of each foreign body. These findings were used to help screen and identify corneal foreign bodies in patients. Corneal smears and scraping cultures were performed in cases of probable corneal infection.

Results

Animal models for the five different foreign particles were established successfully, with each showing distinctive characteristics. These animal results were used to diagnose 41 patients with suspected corneal foreign bodies who were negative by slit lamp examination, but positive by IVCM (observational case series). The most prevalent type of hidden foreign body was plant material (51.2%), followed by metal (29.3%). Ten patients with corneal foreign bodies developed fungal keratitis, found using IVCM.

Conclusions

Laser IVCM is an effective and reliable tool for the diagnosis of hidden corneal foreign bodies.
Evaluation of a Passive Nature Viewing Program Set to Music
Authors: Cadman Sally J.
Published: December 2013

Background: Research has revealed that passive nature viewing (viewing nature scenes without actually being in nature) has many health benefits but little is known about the best method of offering this complementary modality. Aims: The purpose of this pilot program was to evaluate the impact of a passive nature viewing program set to music on stress reduction in adults living in the community. Description and Evaluation: A pre- and postsurvey design along with weekly recordings of stress and relaxation levels were used to evaluate the effect of this passive nature viewing program on stress reduction. Participants watched one of three preselected nature scenes for 5 minutes a day over 1 month and rated their stress and relaxation levels weekly on a 100-mm Visual Analogue Scale before and after viewing the nature DVD. Quantitative analysis were not performed because of the less number of subjects ( n = 10) completing the study. Qualitative analysis found five key categories that have an impact on program use: (a) technology, (b) personal preferences, (c) time, (d) immersion, and (e) use of the program. Conclusions and Implications: Holistic nurses may consider integrating patient preferences and immersion strategies in the design of future passive nature viewing programs to reduce attrition and improve success.
Impact of the spatial and thematic resolution of Holocene anthropogenic land-cover scenarios on modeled soil erosion and sediment delivery rates
Authors: De Brue Hanne , Verstraeten Gert
Published: December 2013

Recent reconstructions of global Holocene vegetation may provide a powerful means to quantify long-term cumulative sediment fluxes induced by anthropogenic land-cover changes. However, the former’s low spatial resolutions question their potential for use in geomorphic models, since sediment redistribution processes operate at much smaller scales. Furthermore, current land-cover reconstructions often do not differentiate the typology of human impact, although the susceptibility of different anthropogenic land uses towards erosion varies greatly. Hence, the present study investigates the sensitivity of a spatially distributed erosion and sediment redistribution model to the spatial and thematic resolution of input land-cover information. The geomorphic model was applied to the Scheldt Basin (~19,000 km2) with low-resolution and high-resolution land-cover input, and subsequently compared to a field-based reference sediment budget of the Dijle subcatchment. Results indicate that low-resolution land-cover information, expressed as proportions of different land-cover types within each grid cell, leads to largely overestimated sediment fluxes. In contrast, spatial allocation of individual land-cover types to a 100-m grid yields more accurate results. These variations in model outcomes are related to differences in landscape connectivity between high- and low-resolution land cover. Moreover, geomorphic model results are non-linearly related to the area under cropland. This indicates that there is not only a need for land-cover reconstructions at high spatial resolution but also that differentiation of anthropogenic land-cover types is essential for accurate geomorphic modeling.
Book Review: The Great Powers versus the Hegemon
Authors: Ahmed Ishtiaq
Published: December 2013

Ahrari, Ehsan, The Great Powers versus the Hegemon (Houndsmills Baskerville: Palgrave Macmillan, 2011). Pp. 266 (including index).
Politiques de lutte contre le paludisme en Casamance, Sénégal: une activité de santé publique soumise aux contextes de conflit et de décentralisation
Authors: Faye Sylvain Landry , le Marcis Frédéric , Samb Fatoumata , Badji Mouhamed
Published: December 2013

Cet article analyse, à partir d’une ethnographie menée à Oussouye (Casamance), la manière dont s’incarnent et se traduisent les politiques nationales sanitaires, dans des contextes locaux spécifiques. Il discute des contraintes que rencontre une politique globalisante, ou plus exactement des points aveugles de cette politique pas suffisamment à l’écoute du contexte, c’est-à-dire, des spécificités régionales et géopolitiques.

Les résultats indiquent que le contexte de la décentralisation et du conflit casamançais ont eu des effets sur le financement, la gestion des activités promotionnelles et la domestication des recommandations officielles de la lutte contre le paludisme. Par ailleurs, celles antérieures laissent des traces, qui s’expriment dans les discours des acteurs et structurent la façon dont ils pensent la lutte aujourd’hui, alors même que cette dernière est supposée s’inscrire dans un autre paradigme. Enfin la dissonance de la prescription d’antipaludiques à Mlomp et Elinkine fait penser à l’existence de « territoires » locaux de la santé où les recommandations nationales cèdent la place aux spécificités contextuelles et locales. Les politiques de santé ne peuvent faire l’économie ni des acteurs, ni des contextes locaux, cependant la prise en compte de la spécificité ne doit certainement pas se faire au détriment d’une certaine notion de « l’être ensemble ».
Analysis of sea-island cotton and upland cotton in response to Verticillium dahliaeinfection by RNA sequencing
Authors: Sun Quan , Jiang Huaizhong , Zhu Xiaoyan , Wang Weina , He Xiaohong , Shi Yuzhen , Yuan Youlu , Du Xiongming , Cai Yingfan
Published: December 2013

Background

Cotton Verticillium wilt is a serious soil-borne vascular disease that causes great economic loss each year. However, due to the lack of resistant varieties of upland cotton, the molecular mechanisms of resistance to this disease, especially to the pathogen Verticillium dahliae, remain unclear.

Results

We used the RNA-seq method to research the molecular mechanisms of cotton defence responses to different races of Verticillium dahliae by comparing infected sea-island cotton and upland cotton. A total of 77,212 unigenes were obtained, and the unigenes were subjected to BLAST searching and annotated using the GO and KO databases. Six sets of digital gene expression data were mapped to the reference transcriptome. The gene expression profiles of cotton infected with Verticillium dahliae were compared to those of uninfected cotton; 44 differentially expressed genes were identified. Regarding genes involved in the phenylalanine metabolism pathway, the hydroxycinnamoyl transferase gene (HCT) was upregulated in upland cotton whereas PAL, 4CL, CAD, CCoAOMT, and COMT were upregulated in sea-island cotton. Almost no differentially expressed genes in this pathway were identified in sea-island cotton and upland cotton when they were infected with V. dahliae V991 and V. dahliae D07038, respectively.

Conclusions

Our comprehensive gene expression data at the transcription level will help elucidate the molecular mechanisms of the cotton defence response to V. dahliae. By identifying the genes involved in the defence response of each type of cotton to V. dahliae, our data not only provide novel molecular information for researchers, but also help accelerate research on genes involved in defences in cotton.
A platform independent RNA-Seq protocol for the detection of transcriptome complexity
Authors: Calabrese Claudia , Mangiulli Marina , Manzari Caterina , Paluscio Anna Maria , Caratozzolo Mariano Francesco , Marzano Flaviana , Kurelac Ivana , D’Erchia Anna Maria , D’Elia Domenica , Licciulli Flavio , Liuni Sabino , Picardi Ernesto , Attimonelli Marcella , Gasparre Giuseppe , Porcelli Anna Maria , Pesole Graziano , Sbisà Elisabetta , Tullo Apollonia
Published: December 2013

Background

Recent studies have demonstrated an unexpected complexity of transcription in eukaryotes. The majority of the genome is transcribed and only a little fraction of these transcripts is annotated as protein coding genes and their splice variants. Indeed, most transcripts are the result of antisense, overlapping and non-coding RNA expression. In this frame, one of the key aims of high throughput transcriptome sequencing is the detection of all RNA species present in the cell and the first crucial step for RNA-seq users is represented by the choice of the strategy for cDNA library construction. The protocols developed so far provide the utilization of the entire library for a single sequencing run with a specific platform.

Results

We set up a unique protocol to generate and amplify a strand-specific cDNA library representative of all RNA species that may be implemented with all major platforms currently available on the market (Roche 454, Illumina, ABI/SOLiD). Our method is reproducible, fast, easy-to-perform and even allows to start from low input total RNA. Furthermore, we provide a suitable bioinformatics tool for the analysis of the sequences produced following this protocol.

Conclusion

We tested the efficiency of our strategy, showing that our method is platform-independent, thus allowing the simultaneous analysis of the same sample with different NGS technologies, and providing an accurate quantitative and qualitative portrait of complex whole transcriptomes.
Long-term effects of stimulants on neurocognitive performance of Taiwanese children with attention-deficit/hyperactivity disorder
Authors: Tsai Ching-Shu , Huang Yu-Shu , Wu Chen-Long , Hwang Fang-Ming , Young Kin-Bao , Tsai Ming-Horng , Chu Shih-Ming
Published: December 2013

Background

Attention-deficit/hyperactivity disorder (ADHD) is a common behavioral and neurocognitive disorder in school-age children. Methylphenidate (MPH) is the most frequently prescribed CNS stimulant for ADHD. The aim of this study is to evaluate the changes in intelligence quotient and domains of neurocognitive function after long-term MPH treatment of Taiwanese children with ADHD.

Methods

The Wechsler Intelligence Scale (WISC-III) was administrated twice at an interval of at least one year for all 171 subjects (6–12 years) and 47 age- and gender-matched children without ADHD. The ADHD-Rating scale and Clinical Global Impression-Severity (CGI-S) were also used at the time of enrolment, and at 6 months and one year later.

Results

Taiwanese children with ADHD had lower Verbal IQ (VIQ) and Full IQ (FIQ) and performed poorly on several subtests of the WISC-III, including Similarities, Vocabulary, and Coding, compared to healthy children without ADHD. After one year of MPH treatment, significant decrements in all scores of the ADHD-Rating scale and CGI-S and increments in several domains of the WISC-III, including FIQ, VIQ, PIQ, Perceptual Organization Index (POI), Picture Completion, Picture Arrangement, Object Assembly, and Digit Span were observed. When the ADHD children under MPH treatment were subdivided into two age groups (6–8 years and 9–12 years), significantly better performance in some subtests and subscales of the WISC-III (such as Similarities, Comprehension, and Object assembly) was found in the 6–8 years age group.

Conclusions

Long-term MPH treatment may improve the neurocognitive profiles of the ADHD children, as seen in their performance in several subtests and in the IQ scores on the WISC-III. And this improvement had no correlation with the decrement of ADHD symptoms. Starting stimulant treatment at as young an age as possible is advised due to the greater benefits in the 6–8 years age group, as seen in this study. More research in this area is also needed to confirm these results.
The association between hyperuricemia and betel nut chewing in Taiwanese men: a cross-sectional study
Authors: Tai Tsai-Sung , Hsu Chih-Cheng , Pai Hsiang-Chu , Liu Wen-Hsin , Hsu Yueh-Han
Published: December 2013

Background

Studies have associated betel nut chewing with cancers, metabolic syndrome, cardiovascular disorders, chronic kidney disease, and proteinuria. This study investigated whether hyperuricemia is associated with betel nut chewing in men who participated in a health check-up program.

Methods

From hospital records, we identified a total of 11,991 men who participated in the health check-up program from 2003 to 2009. They were divided into hyperuricemic group and non-hyperuricemic group. Laboratory tests, medical history, and status of cigarette smoking, alcohol consumption, and betel nut chewing were compared between the 2 groups. We calculated odds ratio (OR) and 95% confidence interval (CI) of hyperuricemia in association with betel nut consumption and other factors.

Results

Compared with the non-hyperuricemic group, the hyperuricemic group was slightly older (59.4 vs. 58.6 years) but less prevalent with betel nut use (11.8 vs. 13.6%, p = 0.003). Multivariable logistic regression analysis showed that hyperuricemia was negatively associated with betel nut chewing (OR 0.75, 95% CI 0.66-0.84), older age (OR 0.84, 95% CI 0.77-0.93), and diabetes mellitus (OR 0.57, 95% CI 0.50-0.64). On the other hand, hyperuricemia was positively associated with body mass index (OR 1.75, 95% CI 1.62-1.90), drinking (OR 1.36, 95% CI 1.25-1.49), hypertension (OR 1.41, 95% CI 1.30-1.52), mixed hyperlipidemia (OR 1.84, 95% CI 1.33-2.54), chronic kidney disease (OR 3.28, 95% CI 2.94-3.65), and proteinuria (OR 1.22, 95% CI 1.08-1.38). Smoking, hypercholesterolemia, and hypertriglyceridemia had no significant association with hyperuricemia.

Conclusion

Our data suggest that betel nut chewing is negatively associated with hyperuricemia.
Metachronous renal vein and artery injure after percutaneous nephrostolithotomy
Authors: Wang Chaojun , Chen Shanwen , Tang Fuqing , Shen Baihua
Published: December 2013

Background

Percutaneous nephrostolithotomy is important approach for kidney stones removal. A percutaneous nephrostomy drainage tube placement is an effective method to stop venous bleeding. Occasionally, the catheter can pierce into the renal parenchyma, and migrate into the renal vein even to the vena cava.

Case presentation

A 66-year-old woman underwent a percutaneous nephrostolithotomy for kidney staghorn stone complicating severe bleeding. A computed tomography angiography showed the percutaneous nephrostomy drainage tube inside the renal vein. The percutaneous nephrostomy drainage tube was withdrawn 3 cm back to the renal parenchyma/sinus/pelvis in stages with the surgical team on standby. Seven days later, the patient developed severe hematuria. Computed tomography angiography demonstrated the pseudoaneurysm located near the percutaneous nephrostomy drainage tube. Pseudoaneurysm is embolized successfully.

Conclusion

Our case shows intravenous misplacement of the nephrostomy tube and subsequent pseudoaneurysm after percutaneous nephrostolithotomy. To our knowledge, this seems to be the first documentation of major bleeding from the injury to both renal vein and artery. The percutaneous nephrostomy drainage tube can be withdrawn back to the renal parenchyma/sinus/pelvis in stages with the surgical team on standby, and the withdrawn distance may vary according to patient and catheter position.
Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
Authors: Matsuda Fumio , Ishii Jun , Kondo Takashi , Ida Kengo , Tezuka Hironori , Kondo Akihiko
Published: December 2013

Background

Isobutanol is an important target for biorefinery research as a next-generation biofuel and a building block for commodity chemical production. Metabolically engineered microbial strains to produce isobutanol have been successfully developed by introducing the Ehrlich pathway into bacterial hosts. Isobutanol-producing baker’s yeast (Saccharomyces cerevisiae) strains have been developed following the strategy with respect to its advantageous characteristics for cost-effective isobutanol production. However, the isobutanol yields and titers attained by the developed strains need to be further improved through engineering of S. cerevisiae metabolism.

Results

Two strategies including eliminating competing pathways and resolving the cofactor imbalance were applied to improve isobutanol production in S. cerevisiae. Isobutanol production levels were increased in strains lacking genes encoding members of the pyruvate dehydrogenase complex such as LPD1, indicating that the pyruvate supply for isobutanol biosynthesis is competing with acetyl-CoA biosynthesis in mitochondria. Isobutanol production was increased by overexpression of enzymes responsible for transhydrogenase-like shunts such as pyruvate carboxylase, malate dehydrogenase, and malic enzyme. The integration of a single gene deletion lpd1 Δ and the activation of the transhydrogenase-like shunt further increased isobutanol levels. In a batch fermentation test at the 50-mL scale from 100 g/L glucose using the two integrated strains, the isobutanol titer reached 1.62 ± 0.11 g/L and 1.61 ± 0.03 g/L at 24 h after the start of fermentation, which corresponds to the yield at 0.016 ± 0.001 g/g glucose consumed and 0.016 ± 0.0003 g/g glucose consumed, respectively.

Conclusions

These results demonstrate that downregulation of competing pathways and metabolic functions for resolving the cofactor imbalance are promising strategies to construct S. cerevisiae strains that effectively produce isobutanol.
Extracellular micronutrient levels and pro-/antioxidant status in trauma patients with wound healing disorders: results of a cross-sectional study
Authors: Blass Sandra C , Goost Hans , Burger Christof , Tolba René H , Stoffel-Wagner Birgit , Stehle Peter , Ellinger Sabine
Published: December 2013

Background

Disorders in wound healing (DWH) are common in trauma patients, the reasons being not completely understood. Inadequate nutritional status may favor DWH, partly by means of oxidative stress. Reliable data, however, are lacking. This study should investigate the status of extracellular micronutrients in patients with DWH within routine setting.

Methods

Within a cross-sectional study, the plasma/serum status of several micronutrients (retinol, ascorbic acid, 25-hydroxycholecalciferol, α-tocopherol, β-carotene, selenium, and zinc) were determined in 44 trauma patients with DWH in addition to selected proteins (albumin, prealbumin, and C-reactive protein; CRP) and markers of pro-/antioxidant balance (antioxidant capacity, peroxides, and malondialdehyde). Values were compared to reference values to calculate the prevalence for biochemical deficiency. Correlations between CRP, albumin and prealbumin, and selected micronutrients were analyzed by Pearson’s test. Statistical significance was set at P < 0.05.

Results

Mean concentrations of ascorbic acid (23.1 ± 15.9 μmol/L), 25-hydroxycholecalciferol (46.2±30.6 nmol/L), β-carotene (0.6 ± 0.4 μmol/L), selenium (0.79±0.19 μmol/L), and prealbumin (24.8 ± 8.2 mg/dL) were relatively low. Most patients showed levels of ascorbic acid (<28 μmol/L; 64%), 25-hydroxycholecalciferol (<50 μmol/L; 59%), selenium (≤ 94 μmol/L; 71%) and β-carotene (<0.9 μmol/L; 86%) below the reference range. Albumin and prealbumin were in the lower normal range and CRP was mostly above the reference range. Plasma antioxidant capacity was decreased, whereas peroxides and malondialdehyde were increased compared to normal values. Inverse correlations were found between CRP and albumin (P < 0.05) and between CRP and prealbumin (P < 0.01). Retinol (P < 0.001), ascorbic acid (P < 0.01), zinc (P < 0.001), and selenium (P < 0.001) were negatively correlated with CRP.

Conclusions

Trauma patients with DWH frequently suffer from protein malnutrition and reduced plasma concentrations of several micronutrients probably due to inflammation, increased requirement, and oxidative burden. Thus, adequate nutritional measures are strongly recommended to trauma patients.
Association between a low IgE response to Phl p 5 and absence of asthma in patients with grass pollen allergy
Authors: Savi Eleonora , Peveri Silvia , Incorvaia Cristoforo , Dell’Albani Ilaria , Marcucci Francesco , Di Cara Giuseppe , Frati Franco
Published: December 2013

Background

The introduction of component-resolved diagnosis was a great advance in diagnosis of allergy. In particular, molecular allergy techniques allowed investigation of the association between given molecular profiles and clinical expression of allergy. We evaluated the possible correlation between the level of specific IgE (sIgE) to single components of Phleum pratense and clinical issues such as the severity of allergic rhinitis (AR) and the presence or absence of asthma.

Methods

The study included 140 patients with rhinitis and/or asthma caused by sensitization to grass pollen. sIgE to Phl p 1, Phl p 5, Phl p 7, and Phl p 12 from Phleum pratense were measured, and the correlation between the stage of AR according to Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines and the presence of asthma was studied by multivariate logistic regression in terms of sIgE and ARIA stage, while univariate logistic regression was used for IgE and a dichotomic classification of asthma as present or absent.

Results

Ten patients had intermittent AR, 48 had mild persistent AR, and 82 had severe persistent AR. Asthma was present in 86 patients and absent in 54. A significant correlation was found between severe persistent AR and presence of asthma (p < 0.01). The only significant correlation between clinical data and sIgE values was that of low values of sIgE to Phl p 5 and absence of asthma (p < 0.01).

Conclusions

This preliminary finding suggests that low values of sIgE to Phl p 5 are correlated with the absence of asthma in patients with grass-pollen induced allergy. The data, provided they are confirmed by further studies, could be useful when selecting patients who are candidates for allergen immunotherapy, since a higher risk of asthma could be used as a selection criterion for using this approach.
Development of HomeSTEAD’s physical activity and screen time physical environment inventory
Authors: Hales Derek , Vaughn Amber E , Mazzucca Stephanie , Bryant Maria J , Tabak Rachel G , McWilliams Christina , Stevens June , Ward Dianne S
Published: December 2013

Background

The home environment has a significant influence on children’s physical activity, sedentary behavior, dietary intake, and risk for obesity and chronic disease. Our understanding of the most influential factors and how they interact and impact child behavior is limited by current measurement tools, specifically the lack of a comprehensive instrument. HomeSTEAD (the Home Self-administered Tool for Environmental assessment of Activity and Diet) was designed to address this gap. This new tool contains four sections: home physical activity and media equipment inventory, family physical activity and screen time practices, home food inventory, and family food practices. This paper will describe HomeSTEAD’s development and present reliability and validity evidence for the first section.

Methods

The ANGELO framework guided instrument development, and systematic literature reviews helped identify existing items or scales for possible inclusion. Refinement of items was based on expert review and cognitive interviews. Parents of children ages 3–12 years (n = 125) completed the HomeSTEAD survey on three separate occasions over 12–18 days (Time 1, 2, and 3). The Time 1 survey also collected demographic information and parent report of child behaviors. Between Time 1 and 2, staff conducted an in-home observation and measured parent and child BMI. Kappa and intra-class correlations were used to examine reliability (test-retest) and validity (criterion and construct).

Results

Reliability and validity was strong for most items (97% having ICC > 0.60 and 72% having r > 0.50, respectively). Items with lower reliability generally had low variation between people. Lower validity estimates (r < 0.30) were more common for items that assessed usability and accessibility, with observers generally rating usability and accessibility lower than parents. Small to moderate, but meaningful, correlations between physical environment factors and BMI, outside time, and screen time were observed (e.g., amount of child portable play equipment in good condition and easy to access was significantly associated with child BMI: r = -0.23), providing evidence of construct validity.

Conclusions

The HomeSTEAD instrument represents a clear advancement in the measurement of factors in the home environment related to child weight and weight-related behaviors. HomeSTEAD, in its entirety, represents a useful tool for researchers from which they can draw particular scales of greatest interest and highest relevance to their research questions.
Effects of a synbiotic on fecal quality, short-chain fatty acid concentrations, and the microbiome of healthy sled dogs
Authors: Gagné Jason W , Wakshlag Joseph J , Simpson Kenneth W , Dowd Scot E , Latchman Shalini , Brown Dawn A , Brown Kit , Swanson Kelly S , Fahey George C
Published: December 2013

Background

Sled dogs commonly suffer from diarrhea. Although multiple etiologies exist there are limited field studies using synbiotics as a supplement to prevent or treat diarrhea. The objective of this study was to examine alterations in fecal quality, short-chain fatty acids (SCFA), and the fecal microbiome in two groups of training sled dogs fed a synbiotic or microcrystalline cellulose placebo. Twenty clinically healthy training sled dogs randomized into two cohorts (9 synbiotic-fed, 8 placebo-fed) for a 6 week prospective study were examined. Fecal pH and fecal short chain fatty acid (SCFA) concentrations were measured and tag-encoded FLX 16S rDNA amplicon pyrosequencing (bTEFAP) and quantitative real-time PCR were performed at baseline (10 d prior to the study) and after 2 weeks of treatment with a total treatment time of 6 weeks. Fecal scores for all dogs were assessed at baseline and every day for 6 wk after initiation of treatment.

Results

Alterations in the fecal microbiome were observed with a significant rise in Lactobacillaceae in the synbiotic group (P = 0.004) after 2 wk of treatment. A positive correlation was found between Lactobacillaceae and overall butyrate concentration (R = 0.62, p = 0.011) in all dogs. After 5 wk of treatment, there was an improved fecal score and fewer days of diarrhea (Χ2 = 5.482, P = 0.019) in the dogs given synbiotic, which coincided with a presumed contagious outbreak shared by all dogs in the study.

Conclusions

Use of this synbiotic results in an increase in presumed beneficial bacterial flora of the host colon which was associated with a decrease in the prevalence of diarrhea in training sled dogs.
A preliminary report on the feasibility of single-port thoracoscopic surgery for diaphragm plication in the treatment of diaphragm eventration
Authors: Wu Hsin-Hung , Chen Chih-Hao , Chang Ho , Liu Hung-Chang , Hung Tzu-Ti , Lee Shih-Yi
Published: December 2013

Introduction

Thoracoscopic surgery is a popular widely used surgical technique in the treatment of common chest conditions. Conventional thoracoscopic surgery utilizes multiple small wounds for carrying out the procedure. Many procedures can also be performed with a single small port wound. In this study, we performed diaphragm plication using the techniques of single-port thoracoscopic surgery.

Materials and methods

From July 1st, 2008 to December 31th, 2011, there were 21 patients admitted to our hospital due to diaphragm eventration. All of them underwent diaphragm plication. The initial 11 patients underwent two-port thoracoscopic surgery while the subsequent 10 patients underwent single-port thoracoscopic surgery.

Results

The side of diaphragm eventration was on the left in all of the cases. The mean operative time was 87.3 minutes and the mean follow-up time was 17 months. There was no procedure-related complication or mortality. The time required for surgery and the postoperative pain scores were similar in the two groups.

Conclusion

Single-port thoracoscopic surgery for diaphragm plication is a safe procedure. It can serve as an alternative to conventional thoracoscopic approaches to diaphragm surgery.
Does Metformin affect ER, PR, IGF-1R, β-catenin and PAX-2 expression in women with diabetes mellitus and endometrial cancer?
Authors: Markowska Anna , Pawałowska Monika , Filas Violetta , Korski Konstanty , Gryboś Marian , Sajdak Stefan , Olejek Anita , Bednarek Wiesława , Śpiewankiewicz Beata , Lubin Jolanta , Markowska Janina
Published: December 2013

Objective

Diabetes mellitus, as a risk factor for endometrial cancer (EC), causes an increase in insulin and IGF-1 concentrations in the blood serum. The increase in insulin and IGF-1 are considered mitogenic factors contributory to cancer development. Studies suggest that metformin has preventive activity, decreasing mortality and the risk of neoplasms. Since estrogen (ER), progesterone (PR) and IGF-1 (IGF-1R) receptor expression and β-catenin and PAX-2 mutations are significant in the development of endometrial cancer, it was decided to study these factors in patients with endometrial cancer and type 2 diabetes mellitus (DM2), and to establish the effects of metformin on their expression.

Methods

The expression of ER, PR, IGF-1R, β-catenin and PAX-2 have been immunohistochemically investigated in 86 type I endometrial cancer specimens. Patients were grouped according to the presence of DM2 and the type of hypoglycemic treatment administered.

Results

Comparing EC patients with DM2 and normal glycemic status, we found increased IGF-1R expression in women with DM2. A decrease in ER expression was noted in women with EC and DM2 receiving metformin as compared to women treated with insulin (p = 0.004). There was no statistically significant difference in PR, IGF-1R, β-catenin and PAX-2 expression among women receiving metformin and other hypoglycemic treatment.

Conclusion

Although epidemiological studies suggest the beneficial role of metformin in many human cancers, there are still few studies confirming its favorable effect on endometrial cancer. Decreased ER expression in patients receiving metformin needs further research to allow evaluation of its clinical significance.
PGC-1α supports glutamine metabolism in breast cancer
Authors: McGuirk Shawn , Gravel Simon-Pierre , Deblois Geneviève , Papadopoli David J , Faubert Brandon , Wegner André , Hiller Karsten , Avizonis Daina , Akavia Uri David , Jones Russell G , Giguère Vincent , St-Pierre Julie
Published: December 2013

Background

Glutamine metabolism is a central metabolic pathway in cancer. Recently, reductive carboxylation of glutamine for lipogenesis has been shown to constitute a key anabolic route in cancer cells. However, little is known regarding central regulators of the various glutamine metabolic pathways in cancer cells.

Methods

The impact of PGC-1α and ERRα on glutamine enzyme expression was assessed in ERBB2+ breast cancer cell lines with quantitative RT-PCR, chromatin immunoprecipitation, and immunoblotting experiments. Glutamine flux was quantified using 13C-labeled glutamine and GC/MS analyses. Functional assays for lipogenesis were performed using 14C-labeled glutamine. The expression of glutamine metabolism genes in breast cancer patients was determined by bioinformatics analyses using The Cancer Genome Atlas.

Results

We show that the transcriptional coactivator PGC-1α, along with the transcription factor ERRα, is a positive regulator of the expression of glutamine metabolism genes in ERBB2+ breast cancer. Indeed, ERBB2+ breast cancer cells with increased expression of PGC-1α display elevated expression of glutamine metabolism genes. Furthermore, ERBB2+ breast cancer cells with reduced expression of PGC-1α or when treated with C29, a pharmacological inhibitor of ERRα, exhibit diminished expression of glutamine metabolism genes. The biological relevance of the control of glutamine metabolism genes by the PGC-1α/ERRα axis is demonstrated by consequent regulation of glutamine flux through the citric acid cycle. PGC-1α and ERRα regulate both the canonical citric acid cycle (forward) and the reductive carboxylation (reverse) fluxes; the latter can be used to support de novo lipogenesis reactions, most notably in hypoxic conditions. Importantly, murine and human ERBB2+ cells lines display a significant dependence on glutamine availability for their growth. Finally, we show that PGC-1α expression is positively correlated with that of the glutamine pathway in ERBB2+ breast cancer patients, and high expression of this pathway is associated with reduced patient survival.

Conclusions

These data reveal that the PGC-1α/ERRα axis is a central regulator of glutamine metabolism in ERBB2+ breast cancer. This novel regulatory link, as well as the marked reduction in patient survival time associated with increased glutamine pathway gene expression, suggests that targeting glutamine metabolism may have therapeutic potential in the treatment of ERBB2+ breast cancer.
Genotypic variation in sorghum [Sorghum bicolor (L.) Moench] exotic germplasm collections for drought and disease tolerance
Authors: Kapanigowda Mohankumar H. , Perumal Ramasamy , Djanaguiraman Maduraimuthu , Aiken Robert M. , Tesso Tesfaye , Prasad P. V. Vara , Little Christopher R.
Published: December 2013

Sorghum [Sorghum bicolor(L.) Moench] grain yield is severely affected by abiotic and biotic stresses during post-flowering stages, which has been aggravated by climate change. New parental lines having genes for various biotic and abiotic stress tolerances have the potential to mitigate this negative effect. Field studies were conducted under irrigated and dryland conditions with 128 exotic germplasm and 12 adapted lines to evaluate and identify potential sources for post-flowering drought tolerance and stalk and charcoal rot tolerances. The various physiological and disease related traits were recorded under irrigated and dryland conditions. Under dryland conditions, chlorophyll content (SPAD), grain yield and HI were decreased by 9, 44 and 16%, respectively, compared to irrigated conditions. Genotype RTx7000 and PI475432 had higher leaf temperature and grain yield, however, genotype PI570895 had lower leaf temperature and higher grain yield under dryland conditions. Increased grain yield and optimum leaf temperature was observed in PI510898, IS1212 and PI533946 compared to BTx642 (B35). However, IS14290, IS12945 and IS1219 had decreased grain yield and optimum leaf temperature under dryland conditions. Under irrigated conditions, stalk and charcoal rot disease severity was higher than under dryland conditions. Genotypes IS30562 and 1790E R had tolerance to both stalk rot and charcoal rot respectively and IS12706 was the most susceptible to both diseases. PI510898 showed combined tolerance to drought and Fusarium stalk rot under dryland conditions. The genotypes identified in this study are potential sources of drought and disease tolerance and will be used to develop better adaptable parental lines followed by high yielding hybrids.
Effect of microtitanium impregnated tape on the recovery of triceps surae musculotendinous function following strenuous running
Authors: Hughes Jonathan D. , Fink Philip W. , Graham David F. , Rowlands David S.
Published: December 2013

We previously reported increased running economy and joint range of motion (ROM) during subsequent exercise performed 48-h following strenuous exercise while wearing garments containing micro-titanium particles generated from high-pressure aqueous processing of titanium (AQUA TITANTM). Here we utilised an isolated plantarflexion triceps surae model and AQUA TITAN-treated flexible tape to determine if dermal application of the micro-titanium could account for meaningful changes in functional properties of the musculotendinous unit. In a randomised double-blind crossover, 20 trained men day 1, baseline measures, AQUA TITAN or placebo tape covering the triceps surae, intermittent high-intensity treadmill running; day 2, rest; day 3, post-stress post-treatment outcome measures. Outcomes comprised: plantarflexion ROM via isokinetic dynamometry; short latency reflex from electromyography; Achilles tendon stiffness from isometric dynamometry, ultrasonography (Achilles-medial-gastrocnemius junction), motion analysis, and force-length modelling. High-intensity exercise with placebo tape reduced tendon stiffness (-16.5%; 95% confidence limits ±8.1%; small effect size), relative to non-taped baseline, but this effect was negligible (-5.9%; ±9.2%) with AQUA TITAN (AQUA TITAN-placebo difference -11.3%; ±11.6%). Change in latency relative to baseline was trivial with placebo (1.6%; ±3.8%) but large with AQUA TITAN (-11.3%; ±3.3%). The effects on ROM with AQUA TITAN (1.6%; ±2.0%) and placebo were trivial (-1.6% ±1.9%), but the small difference (3.1%; ±2.7%) possibly greater with AQUA TITAN. AQUA TITAN tape accelerated the reflex response and attenuated reduced Achilles tendon stiffness following fatiguing exercise. Altered neuromuscular control of tendon stiffness via dermal application of micro-titanium treated materials may facilitate restoration of musculotendinous contractile performance following prior strenuous exercise.
Graph analysis of β2 adrenergic receptor structures: a “social network” of GPCR residues
Authors: Sheftel Samuel , Muratore Kathryn E , Black Michael , Costanzi Stefano
Published: December 2013

Purpose

G protein-coupled receptors (GPCRs) are a superfamily of membrane proteins of vast pharmaceutical interest. Here, we describe a graph theory-based analysis of the structure of the β2adrenergic receptor (β2AR), a prototypical GPCR. In particular, we illustrate the network of direct and indirect interactions that link each amino acid residue to any other residue of the receptor.

Methods

Networks of interconnected amino acid residues in proteins are analogous to social networks of interconnected people. Hence, they can be studied through the same analysis tools typically employed to analyze social networks – or networks in general – to reveal patterns of connectivity, influential members, and dynamicity. We focused on the analysis of closeness-centrality, which is a measure of the overall connectivity distance of the member of a network to all other members.

Results

The residues endowed with the highest closeness-centrality are located in the middle of the seven transmembrane domains (TMs). In particular, they are mostly located in the middle of TM2, TM3, TM6 or TM7, while fewer of them are located in the middle of TM1, TM4 or TM5. At the cytosolic end of TM6, the centrality detected for the active structure is markedly lower than that detected for the corresponding residues in the inactive structures. Moreover, several residues acquire centrality when the structures are analyzed in the presence of ligands. Strikingly, there is little overlap between the residues that acquire centrality in the presence of the ligand in the blocker-bound structures and the agonist-bound structures.

Conclusions

Our results reflect the fact that the receptor resembles a bow tie, with a rather tight knot of closely interconnected residues and two ends that fan out in two opposite directions: one toward the extracellular space, which hosts the ligand binding cavity, and one toward the cytosol, which hosts the G protein binding cavity. Moreover, they underscore how interaction network is by the conformational rearrangements concomitant with the activation of the receptor and by the presence of agonists or blockers.
Essential Health Benefits and the Affordable Care Act: Law and Process
Authors: Bagley Nicholas , Levy Helen
Published: December 2013

Starting in 2014, the Affordable Care Act (ACA) will require private insurance plans sold in the individual and small-group markets to cover a roster of “essential health benefits.” Precisely which benefits should count as essential, however, was left to the discretion of the Department of Health and Human Services (HHS). The matter was both important and controversial. Nonetheless, HHS announced its policy by posting on the Internet a thirteen-page bulletin stating that it would allow each state to define essential benefits for itself. On both substance and procedure, the move was surprising. The state-by-state approach departed from the uniform, federal standard that the ACA appears to anticipate and that informed observers expected HHS to adopt. And announcing the policy through an Internet bulletin appeared to allow HHS to sidestep traditional administrative procedures, including notice and comment, immediate review in the courts, and White House oversight. This article explores two questions. First, is the state-by-state approach a lawful exercise of HHS's authority? Second, did HHS in fact evade the procedural obligations that are meant to shape the exercise of its discretion?
The role of hydrodynamics in structuring in situ ammonium uptake within a submerged macrophyte community
Authors: Morris Edward P. , Peralta Gloria , Van Engeland Tom , Bouma Tjeerd J. , Brun Fernando G. , Lara Miguel , Hendriks Iris E. , Benavente Javier , Soetaert Karline , Middelburg Jack J. , Lucas Perez‐Llorens J.
Published: December 2013

Lay Abstract

Aquatic macrophytes (such as macroalgae and sea grasses) are able to take up nutrients directly from the surrounding water in which they inhabit. How fast nutrients are transported into their structures (such as leaves or fronds) can be influenced by water flow. However, macrophytes tend to form canopies made up of many individual structures, and the presence of these canopies has large effects on local water flow. This implies that in shallow regions the architecture of different canopies may well be a key factor in setting rates of nutrient transport to the sea floor. Predicting these effects is complicated because in many natural systems macrophyte communities often form patchy distributions consisting of bare and vegetated areas, as well as patches of different species with different canopy types. Here we use a new application of stable isotope labeling to directly measure the in situ uptake of ammonium at the transition between short, dense Caulerpa prolifera (a macroalga) and tall, sparse Cymodocea nodosa (a sea grass) canopies. We found that as water flowed over the dense macroalgae into the sparse sea grass canopy, it accelerated, resulting in horizontal and vertical variations in water velocity and mixing that appeared to explain variations in the ammonium uptake of the different types of organisms living on and within the canopies. These results highlight how the interplay between the physical structure of organisms and flow may determine the nutrient transport of individual species within macrophyte communities, creating nutrient transport microhabitats that may influence biodiversity and ecosystem functioning.
Involvement of Delta/Notch signaling in zebrafish adult pigment stripe patterning
Authors: Hamada Hiroki , Watanabe Masakatsu , Lau Hiu Eunice , Nishida Tomoki , Hasegawa Toshiaki , Parichy David M. , Kondo Shigeru
Published: December 2013

The skin pigment pattern of zebrafish is a good model system in which to study the mechanism of biological pattern formation. Although it is known that interactions between melanophores and xanthophores play a key role in the formation of adult pigment stripes, molecular mechanisms for these interactions remain largely unknown. Here, we show that Delta/Notch signaling contributes to these interactions. Ablation of xanthophores in yellow stripes induced the death of melanophores in black stripes, suggesting that melanophores require a survival signal from distant xanthophores. We found that deltaC and notch1a were expressed by xanthophores and melanophores, respectively. Moreover, inhibition of Delta/Notch signaling killed melanophores, whereas activation of Delta/Notch signaling ectopically in melanophores rescued the survival of these cells, both in the context of pharmacological inhibition of Delta/Notch signaling and after ablation of xanthophores. Finally, we showed by in vivo imaging of cell membranes that melanophores extend long projections towards xanthophores in the yellow stripes. These data suggest that Delta/Notch signaling is responsible for a survival signal provided by xanthophores to melanophores. As cellular projections can enable long-range interaction between membrane-bound ligands and their receptors, we propose that such projections, combined with direct cell-cell contacts, can substitute for the effect of a diffusible factor that would be expected by the conventional reaction-diffusion (Turing) model.