bluuebunny/crossref_metadata_2025 huggingface dataset:
Topology and Impedance Identification Method of Low-Voltage Distribution Network Based on Smart Meter Measurements
Authors: Tong Li , Chai Weijian , Wu Dongqi
Published: April 2022

In recent years, distributed photovoltaic facilities and distributed energy storage are widely connected to a low-voltage distribution network, which makes the topology of the low-voltage distribution network change faster, and the online identification and monitoring of the impedance of each branch are becoming more difficult. With the widespread installation of new distribution automation terminals and smart electricity meters, the technical gap of power data collection has been filled, the state perception ability of the distribution network has been improved, and the data support for further exploration of the topology and impedance of the distribution network has been provided. Therefore, the low-voltage distribution network topology and impedance identification method based on smart meter measurements, the physical topology model of distribution transformer-branch-meter box-user and the impedance exclusion model are proposed. Line loss and branch node power flow are calculated on the basis of the two models. Finally, the low-voltage distribution network topology recognition algorithm and power flow partition backtracking impedance estimation technology are developed based on the data mining method of distribution transformer terminal and smart meter. The example results show that the physical topology model of distribution transformer-branch-meter box-user and the impedance exclusion model proposed in this study can effectively identify the branch topology and impedance distribution in the low-voltage distribution network with a large amount of measurement information, realize the online perception of the distribution network, and provide the basis for the fine management of the power grid and line loss.
Healthy Eating Index Scores, Body Mass Index and Abdominal Obesity Among Asian Americans: NHANES 2011–2018
Authors: Thomas Deena B. , Leak Tashara M.
Published: April 2022

Objective

Obesity rates are increasing among Asian Americans so, the objective of this study was to examine associations between Healthy Eating Index scores (a measure of adherence to the Dietary Guidelines for Americans (DGAs)) and body mass index (BMI) and waist circumference (measure of abdominal obesity) among Asian American adults.

Methods

Included in these analyses were Asian Americans ≥20 years from the National Health and Nutrition Examination Survey (2011–18) who had: (1) two 24 h diet recalls to calculate HEI and HEI subcomponent scores, (2) height and weight data to calculate BMI overweight ≥25 kg/m2 and obese ≥ 30kg/m (2), and (3) waist circumference data (high risk ≥ 80 cm for women and ≥ 90 cm for men; n = 1024 women and n = 904 men). Multinomial logistic regression models were run with BMI as the outcome and logistic regression models were run with waist circumference as the outcome, controlling for age, income, marital status, education, and physical activity.

Results

Among women, a diet with greater adherence to the DGAs for total fruit, fatty acids and total protein was associated with lower likelihood of developing obesity, but a diet with greater adherence to the DGAs in terms of whole grains and sodium (i.e., lower sodium intake) was associated with a higher likelihood of obesity. Among men, greater adherence to the DGAs for fatty acids was associated with a lower likelihood of obesity but greater adherence to the DGAs in terms of whole grains and sodium (i.e., lower sodium intake) was associated with a greater likelihood of obesity.

Conclusions

Future research is needed to examine associations between consumption of specific foods and beverages and how they are associated with BMI and waist circumference among Asian American adults. Furthermore, there is a need culturally relevant obesity prevention interventions for Asian American adults, especially interventions that take into consideration cultural food norms of specific Asian American subgroups.
Potential Futures for Coastal Wolves and Their Ecosystem Services in Alaska, With Implications for Management of a Social-Ecological System
Authors: Gilbert Sophie L. , Haynes Trevor , Lindberg Mark S. , Albert David M. , Kissling Michelle , Lynch Laurel , Person Dave
Published: April 2022

Carnivores across much of the world are declining, leading to loss of biodiversity as well as the ecosystem services carnivores provide. In 2020, the Alexander Archipelago (AA) wolf was petitioned for protection under the U.S. Endangered Species Act (ESA) for the third time in 30 years. Concerns included habitat alteration from industrial timber harvest and subsequent declines in prey (deer), human-caused mortality, climate change, and genetic inbreeding. However, the underlying biogeography and ecology of these wolves continues to suggest resiliency across the subspecies’ range, even though local populations may go extinct. If local wolf populations go extinct, it will result in loss of their ecosystem services (e.g., interactions of wolves with their prey, which prevents over-browsing and protects carbon sequestration in soils and trees), which will likely have major consequences for the local social-ecological system. Here, we updated a model we constructed for the last ESA listing process (2015) to examine the dynamics of wolf and deer populations on Prince of Wales Island (the primary geographic focus of all three petitions) in response to future environmental and management scenarios developed with stakeholders. Further, we considered how changes in deer abundance impact predation services (prevention of over-browsing by deer). We found that wolf populations generally persisted over 30 years, but dropped below an effective population size of 50 wolves in 10–98% of years simulated. Low wolf abundance resulted in higher deer abundance, which increased hunting opportunity, but also browsing damages (e.g., 19% of areas would be over-browsed if wolf harvest caps are removed, and >30% of areas would be over-browsed if wolves go extinct). Human harvest of wildlife was a key regulator of abundance and ecosystem services within the coastal rainforest social-ecological system; wolf abundance was most affected by wolf harvest regulations; and deer harvest restrictions increased wolf and deer abundances, but also greatly increased browsing impacts (>70% of areas heavily browsed if hunting ceased). Our findings support an integrated approach to management of this social-ecological system, such that social and ecological sciences are both used to monitor important components of the system (e.g., measuring public sentiment and likelihood of poaching, alongside wolf and deer numbers). Integration and adaptive approaches are needed to ensure that the many ecosystem services humans depend on are valued, conserved, and restored, including the cryptic predation services wolves have historically provided to the timber industry via reduced browsing pressure by deer.
A Novel Risk Model Based on Autophagy-Related LncRNAs Predicts Prognosis and Indicates Immune Infiltration Landscape of Patients With Cutaneous Melanoma
Authors: Shu Qi , Zhou Yi , Zhu Zhengjie , Chen Xi , Fang Qilu , Zhong Like , Chen Zhuo , Fang Luo
Published: April 2022

Cutaneous melanoma (CM) is a malignant tumor with a high incidence rate and poor prognosis. Autophagy plays an essential role in the development of CM; however, the role of autophagy-related long noncoding RNAs (lncRNAs) in this process remains unknown. Human autophagy-related genes were extracted from the Human Autophagy Gene Database and screened for autophagy-related lncRNAs using Pearson correlation. Multivariate Cox regression analysis was implemented to identify ten autophagy-related lncRNAs associated with prognosis, and a risk model was constructed. The Kaplan–Meier survival curve showed that the survival probability of the high-risk group was lower than that of the low-risk group. A novel predictive model was constructed to investigate the independent prognostic value of the risk model. The nomogram results showed that the risk score was an independent prognostic signature that distinguished it from other clinical characteristics. The immune infiltration landscape of the low-risk and high-risk groups was further investigated. The low-risk groups displayed higher immune, stromal, and ESTIMATE scores and lower tumor purity. The CIBERSORT and single sample gene set enrichment analysis (ssGSEA) algorithms indicated a notable gap in immune cells between the low- and high-risk groups. Ten autophagy-related lncRNAs were significantly correlated with immune cells. Finally, Gene Set Enrichment Analysis (GSEA) and Gene Set Variation Analysis (GSVA) results demonstrated that autophagy-related lncRNA-mediated and immune-related signaling pathways are crucial factors in regulating CM. Altogether, these data suggest that constructing a risk model based on ten autophagy-related lncRNAs can accurately predict prognosis and indicate the tumor microenvironment of patients with CM. Thus, our study provides a new perspective for the future clinical treatment of CM.
Feasibility and Acceptability of a Guided Self-Help, Text-Messaging Intervention to Promote Positive Body Image of Emerging Adult Women
Authors: Rogers Courtney B. , Webb Jennifer B. , Bauert Lia , Carelock Jordan
Published: April 2022

The predominant approach of existing eating disorder prevention programs targets risk factors for development; furthermore, burgeoning evidence suggests that promotion of protective factors against eating disorders (e.g., positive body image) is also a worthy avenue for prevention efforts. The present study considered existing literature gaps in the design of an 8-week guided self-help intervention meant to address the risk for disordered eating through the improvement of positive body image and enhancement of current adaptive functioning. The intervention incorporated elements of weight-inclusive health promotion (e.g., Health at Every Size; HAES) alongside positive psychology and third-wave behavioral interventions [e.g., self-compassion, mindful eating, Acceptance and Commitment Therapy (ACT)] to promote engagement in mindful-self-care. This mixed-methods study evaluated the feasibility and acceptability of the text-messaging based intervention in a diverse sample of cisgender college women (N = 30; 30% Black; 30% bisexual) at risk for disordered eating. Results indicated a high level of engagement and satisfaction with the intervention. Proof of concept was preliminarily supported by the observed significant changes in variables of interest (i.e., body appreciation, positive embodiment, mindful self-care, intuitive eating, self-compassion, disordered eating, and body image dissatisfaction) across the intervention. Overall, results of this study suggest that the use of a guided self-help program based in technology which seeks to reduce risk factors for disordered eating while also supporting adaptive functioning may be indicated for emerging adult women. This article will discuss how the present study provides the groundwork for continued development of innovative and remotely accessible interventions which promote positive body image.
Survival Prognosis, Tumor Immune Landscape, and Immune Responses of ADAMTS14 in Clear Cell Renal Cell Carcinoma and Its Potential Mechanisms
Authors: Chen Yinhao , Ji Hao , Liu Shouyong , Xing Qianwei , Zhu Bingye , Wang Yi
Published: April 2022

Background

ADAMTS14 played a crucial role in the formation and development of various cancers. Currently, no associations had been revealed between ADAMTS14 and clear cell renal cell carcinoma (ccRCC). Hence, this study was designed to assess the prognostic values and immunological roles of ADAMTS14 in ccRCC and to reveal its potential mechanisms.

Methods

ADAMTS14-related expression profiles and related clinical data were downloaded from The Cancer Genome Atlas (TCGA) dataset, validated by the ICGC dataset, qRT-PCR, and immunohistochemistry. We utilized gene set enrichment analysis (GSEA) to find potentially ADAMTS14-related pathways and applied univariate/multivariate Cox regression analyses to identify independent factors significantly related to overall survival (OS) for ccRCC. A nomogram consisted of independent prognostic factors was also conducted. We further explored the associations between ADAMTS14 with immunity and revealed its potential mechanisms.

Results

ADAMTS14 displayed a higher expression in ccRCC tumor than in adjacent normal tissues, and further validated results of the ICGC dataset; qRT-PCR and immunohistochemistry remained consistent (all p < 0.05). Moreover, elevated ADAMTS14 expression was significantly associated with poor OS (p < 0.001). Through univariate/multivariate Cox regression analyses, ADAMTS14 was found to be an independent prognostic factor for ccRCC (both p < 0.05) and GSEA identified several signaling pathways including INSULIN, MTOR, and PPAR pathways. The nomogram based on independent prognostic factors was successfully established and well evaluated. Moreover, the expression of ADAMTS14 was remarkably associated with immune checkpoint molecules, tumor mutational burden (TMB), immune cells, and tumor immune microenvironment (all p < 0.05). Results from TIDE and TCIA showed that highly expressed ADAMTS14 could predict worse efficacy of immunotherapy (all p < 0.05). As for its potential mechanisms, we also revealed several LncRNA/RNA binding protein (RBP)/ADAMTS14 mRNA networks.

Conclusions

ADAMTS14 was found to play oncogenic roles in ccRCC and to be significantly associated with immunity. Several LncRNA/RBP/ADAMTS14 mRNA networks were also identified for its potential mechanisms.
m6A Reader Igf2bp1 Regulates the Inflammatory Responses of Microglia by Stabilizing Gbp11 and Cp mRNAs
Authors: Ding Lu , Wu Huiran , Wang Yi , Li Yun , Liang Zhanping , Xia Xiaohuan , Zheng Jialin C.
Published: April 2022

Microglia are brain resident cells that function as brain phagocytic macrophages. The inflammatory responses of microglia induced by pathologic insults are key regulators in the progression of various neurological disorders. Currently, little is known about how these responses are regulated intrinsically. Here, it is observed that LPS-activated microglia exhibit distinct N6-methyladenosine (m6A) methylation patterns that are positively correlated with the expression patterns of corresponding mRNAs. High-throughput analyses and molecular studies both identified Igf2bp1 as the most significantly regulated m6A modifiers in activated microglia. Perturbation of function approaches further indicated Igf2bp1 as a key mediator for LPS-induced m6A modification and microglial activation presumablyviaenhancing the m6A methylation and stability ofGbp11andCpmRNAs. Thus, our study provides a possible mechanism for the m6A methylation-mediated microglia regulation and identifies Igf2bp1 as a potential target for modulating the inflammatory responses of microglia.
m6A Regulator-Mediated Tumour Infiltration and Methylation Modification in Cervical Cancer Microenvironment
Authors: Zhang Wenyi , Xiao Pei , Tang Jiayi , Wang Rui , Wang Xiangdong , Wang Fengxu , Ruan Junpu , Yu Shali , Tang Juan , Huang Rongrong , Zhao Xinyuan
Published: April 2022

Background

N6-methyladenosine (m6A) is the most abundant internal mRNA modification in eukaryotic cells. There is accumulating evidence that m6A methylation can play a significant role in the early diagnosis and treatment of cancers. However, the mechanism underlying the involvement of m6A in cervical cancer remains unclear.

Methods

Here, we examined the m6A modification patterns of immune cells in the tumour microenvironments (TMEs) of 306 patients with cervical cancer from The Cancer Genome Atlas dataset and analysed the relations between them according to 32 m6A regulators. Immune infiltration in the TME of cervical cancer was analysed using the CIBERSORT algorithm and single-sample gene set enrichment analysis. The m6Ascore was structured though principal component analysis.

Results

Two different m6A modification patterns were detected in 306 patients with cervical cancer, designated as m6Acluster A and B. The immune cell infiltration characteristics and biological behaviour differed between the two patterns, with m6Acluster A showing a higher level of immune infiltration. The samples were also divided into two genomic subtypes according to 114 m6A regulatory genes shown to be closely correlated with prognosis on univariate Cox regression analysis. Survival analysis showed that gene cluster B was related to better survival than gene cluster A. Most of the m6A regulators showed higher expression in gene cluster B than in gene cluster A. Single-sample gene set enrichment analysis indicated a higher level of immune cell infiltration in gene cluster A. The m6Ascore signature was examined to determine the m6A modification patterns in cervical cancer. Patients with a high m6Ascore showed better survival, while the low m6Ascore group had a higher mutation frequency and better response to treatment.

Conclusions

This study showed that m6A modification patterns play important roles in cervical cancer. Analysis of m6A modification patterns will yield an improved understanding of the TME in cervical cancer, and facilitate the development of better immunotherapy strategies.
Evaluation of Humoral and Cellular Immune Responses to the SARS-CoV-2 Vaccine in Patients With Common Variable Immunodeficiency Phenotype and Patient Receiving B-Cell Depletion Therapy
Authors: Antolí Arnau , Rocamora-Blanch Gemma , Framil Mario , Mas-Bosch Virgínia , Navarro Sergio , Bermudez Carla , Martinez-Yelamos Sergio , Dopico Eva , Calatayud Laura , Garcia-Muñoz Nadia , Hernández-Benítez Luis Humberto , Riera-Mestre Antoni , Bas Jordi , Masuet-Aumatell Cristina , Rigo-Bonnin Raúl , Morandeira Francisco , Solanich Xavier
Published: April 2022

Introduction

SARS-CoV-2 vaccines’ effectiveness is not yet clearly known in immunocompromised patients. This study aims to assess the humoral and cellular specific immune response to SARS-CoV-2 vaccines and the predictors of poor response in patients with common variable immunodeficiency (CVID) phenotype and in patients treated with B-cell depletion therapies (BCDT), as well as the safety of these vaccines.

Methods

From March to September 2021, we performed a prospective study of all adult patients who would receive the SARS-CoV-2 vaccination and were previously diagnosed with (i) a CVID syndrome (CVID phenotype group; n=28) or (ii) multiple sclerosis (MS) treated with B-cell depleting therapies three to six months before vaccination (BCD group; n=24). Participants with prior SARS-CoV-2 infection; or prior SARS-CoV-2 vaccine administration; or use of any immunosuppressant (except BCDT in MS group) were excluded. A group of subjects without any medical condition that confers immunosuppression and who met all study criteria was also assessed (control group; n=14). A chemiluminescence immunoassay was used to determine pre- and post-SARS-CoV-2 vaccine anti-S IgG antibodies. T-cell specific response was assessed by analysis of pre- and post-SARS-CoV-2 vaccination blood samples with an interferon-gamma release assay. The baseline blood sample also included several biochemical, haematological and immunological analyses.

Results

SARS-CoV-2 vaccines are safe in immunocompromised patients, although their effectiveness was lower than in healthy individuals. CVID phenotype patients showed impaired humoral (29%) and cellular (29%) response, while BCD patients fundamentally presented humoral failure (54%). Low IgA values, low CD19+ peripheral B cells, low switched memory B cells, and a low CD4+/CD8+ ratio were predictors of inadequate specific antibody response in CVID phenotype patients. No factor was found to predict poor cellular response in CVID phenotype patients, nor a defective humoral or cellular response in BCD patients.

Conclusion

The effectiveness of SARS-CoV-2 vaccines in CVID phenotype and BCD patients is lower than in healthy individuals. Knowledge of predictive factors of humoral and cellular response failure in immunocompromised patients could be very useful in clinical practice, and thus, studies in this regard are clearly needed.
Effects of Shear Stress and Salinity Stratification on Floc Size Distribution During the Dry Season in the Modaomen Estuary of the Pearl River
Authors: Huang Jie , Wang Simin , Li Xinran , Xie Rongyao , Sun Jianxiong , Shi Benwei , Liu Feng , Cai Huayang , Yang Qingshu , Zheng Zhaoyong
Published: April 2022

The floc size distribution of fine cohesive sediments in estuaries varies spatiotemporally within assorted physical, chemical, and biological factors. However, the distribution of different floc fractions that are affected by shear stress and salinity stratification has not yet been thoroughly investigated. This study intends to clarify the floc size distribution within the influences of turbulent shear rate and salinity stratification, and the implications for the flocculation process during the dry season in the Modaomen Estuary of the Pearl River. The decomposition of multimodal floc size distributions (FSDs) indicates that the floc fractions were composed of macroflocs (Macro), microflocs (Micro), Flocculi, and primary particles (Pp). Macro generally existed among the upper and middle layers, but smaller flocs, i.e., Micro, Flocculi, and Pp, were mostly concentrated in the bottom layer. The results agreed that the flocculation and deflocculation processes were dominant in the upper and bottom layers, respectively. In response to strong turbulent shear rates, FSDs in the bottom layer skewed toward small sizes and had a dual-peak tendency with frequent floc exchanges between Pp and Micro, then being Pp-dominant but converting to Micro when turbulent shear rates decreased. With impeded vertical mixing by salinity stratification, the FSDs in the upper or middle layers skewed toward a larger particle size with single peaks and lack of exchange among different floc fractions, leading to Macro dominance with a larger volume concentration and median size. In addition, turbulence mixing dramatically interfered with the good mixing of floc fractions amidst the vertical water column, with a low-salinity condition greatly affecting the formation of Macro in the bottom layer within proper turbulent shear rates. This study explores the effects of shear stress and salinity stratification on the flocculation process in the Modaomen Estuary, which contributes to a better understanding of sediment movement in a complex estuarine environment.
Temporal Genetic Stability Despite Decades of Overexploitation for Large Yellow Croaker in the East China Sea
Authors: Xu Zhe , Dou Shuo-Zeng , Ding Shao-Xiong , Liu Jin-Xian
Published: April 2022

Almost all the commercial fishery stocks have been overexploited, resulting in dramatic populations decline and phenotypic change. Understanding the genetic effects of overexploitation have important implications for the conservation and management of fishery resources. In the present study, we investigated temporal changes of genetic diversity and spatio-temporal genetic structure in the heavily exploited large yellow croaker (Larimichthys crocea) in the East China Sea, through microsatellite analysis of historical and contemporary samples. Despite the drastic population decline, we found no significant decline in measures of genetic diversity (Ar, He and FIS). The contemporary effective population sizes were still large enough and genetic drift was not strong enough to reduce the genetic diversity of large yellow croaker significantly in the East China Sea. Furthermore, no evidence of spatio-temporal genetic structure was detected. All the analysis of genetic structure indicated that the proportion of variance explained by temporal factors was small and similar with that of spatial factors. We therefore concluded that the genetic structure of the large yellow croaker in the East China Sea has been essentially stable over the time-span of 60 years. These results suggested that the drastic population declines did not change genetic composition of large yellow croaker in the East China Sea. Based on the long-term stable temporal pattern of genetic composition, we suggested that fishing restrictions and habitat restoration should be the most direct and effective management strategy for the recovery of large yellow croaker stocks.
Uniformity Prediction of Bending Stiffness of Composite Space Mirrors With Ply Angle Misalignments by Deflection Angle
Authors: Liu Qingnian , Shao Yingfeng , Li Long , Cai Yong , Song Fan
Published: April 2022

Based on the classical lamination theory, the principal moment direction of the laminate is derived. It is 45° apart from the generalized ply angle, which is the orientation of the ply where the ply angle misalignment occurs nominally. After adding a fixed one-ply angle or random multiple ply angle misalignments to six quasi-isotropic and symmetric stacking sequences, out-of-plane deformations of mirrors were simulated and compared. The result shows an angle difference between the principal moment and curvature directions. It is referred to as the deflection angle, approximately appearing as a trigonometric curve, which varies with laminates and is dependent on the generalized ply angle within each laminate. The deflection angle directly reflects the consistency of bending stiffness or laminate bending anisotropy; the smaller the deflection angle, the better will be the uniformity of bending stiffness. Consequently, it is an efficient approach to obtain a laminate with more uniformity of bending stiffness by comparing the deflection angle. These results enrich the research of out-of-plane deformation and could objectively promote the study of quasi-isotropic laminates for bending stiffness.
Liquid Biopsy in Squamous Cell Carcinoma of the Esophagus and of the Head and Neck
Authors: Iacob Razvan , Mandea Matei , Iacob Speranta , Pietrosanu Catalina , Paul Doru , Hainarosie Razvan , Gheorghe Cristian
Published: April 2022

Squamous cell carcinomas of the esophagus (ESCC) and of the head and neck (HNSCC) are two neoplasms that share common risk factors and have the same embryological origin, but a very different prognosis, the 5-year survival of HNSCC being almost double (40–50%) compared to the 5-year survival of ESCC (20%). Current guidelines emphasize the importance of screening for ESCC in patients diagnosed with head and neck cancers. A liquid biopsy is a novel tool for diagnosis, prognostic stratification, and personalized therapy. Liquid biopsy biomarkers for these two malignancies could help both their early detection, facilitate residual disease identification, and provide prognosis information. The present systematic review of the literature was aimed at describing the liquid biopsy biomarkers present in these two malignancies, with an emphasis on potential clinical applications.
Dynamics of Hepatitis B Virus Pregenomic RNA in Chronic Hepatitis B Patients With Antiviral Therapy Over 9 Years
Authors: Pan Jiali , Tian Yu , Xu Jinghang , Luo Hao , Tan Ning , Han Yifan , Kang Qian , Chen Hongyu , Yang Yuqing , Xu Xiaoyuan
Published: April 2022

Serum hepatitis B virus pregenomic RNA (HBV pgRNA) is a potential biomarker that is correlated with covalently closed circular DNA. The long-term dynamics of HBV pgRNA in patients with chronic hepatitis B need to be explored. One hundred naïve nucleos(t)ide analog-treated patients with chronic hepatitis B were enrolled to analyze the dynamics of HBV pgRNA over 9 years. The positive rates of HBV pgRNAs declined gradually and showed biphasic kinetics. Serum HBV pgRNA levels in patients treated with entecavir became negative later than those treated with adefovir or lamivudine. Patients who remain positive for HBV pgRNA after 9 years of treatment may have higher viral transcription efficiencies. The reverse transcription efficiency of hepatitis B e-antigen (HBeAg)-positive patients was higher than that of HBeAg-negative patients at baseline and showed no difference after 24-week nucleos(t)ide analog treatment. The trajectory of serum HBV pgRNA-negative transformation differs in patients with different characteristics. Long-term dynamic monitoring of serum HBV pgRNA levels has significance in hepatitis B treatment.
The Kidney Donor Profile Index (KDPI) Correlates With Histopathologic Findings in Post-reperfusion Baseline Biopsies and Predicts Kidney Transplant Outcome
Authors: Bachmann Quirin , Haberfellner Flora , Büttner-Herold Maike , Torrez Carlos , Haller Bernhard , Assfalg Volker , Renders Lutz , Amann Kerstin , Heemann Uwe , Schmaderer Christoph , Kemmner Stephan
Published: April 2022

Background

The increasing organ shortage in kidney transplantation leads to the necessity to use kidneys previously considered unsuitable for transplantation. Numerous studies illustrate the need for a better decision guidance rather than only the classification into kidneys from standard or expanded criteria donors referred to as SCD/ECD-classification. The kidney donor profile index (KDPI) exhibits a score utilizing a much higher number of donor characteristics. Moreover, graft biopsies provide an opportunity to assess organ quality.

Methods

In a single center analysis 383 kidney transplantations (277 after deceased and 106 after living donation) performed between January 1st, 2006, and December 31st, 2016, retrospectively underwent SCD/ECD and KDPI scoring. Thereby, the quality of deceased donor kidneys was assessed by using the KDPI and the living donor kidneys by using the living KDPI, in the further analysis merged as (L)KDPI. Baseline biopsies taken 10 min after the onset of reperfusion were reviewed for chronic and acute lesions. Survival analyses were performed using Kaplan-Meier analysis and Cox proportional hazards analysis within a 5-year follow-up.

Results

The (L)KDPI correlated with glomerulosclerosis (r = 0.30, p < 0.001), arteriosclerosis (r = 0.33, p < 0.001), interstitial fibrosis, and tubular atrophy (r = 0.28, p < 0.001) as well as the extent of acute tubular injury (r = 0.20, p < 0.001). The C-statistic of the (L)KDPI concerning 5-year death censored graft survival was 0.692. Around 48% of ECD-kidneys were classified as (L)KDPI<85%. In a multivariate Cox proportional hazard analysis including (preformed) panel reactive antibodies, cold ischemia time, (L)KDPI, and SCD/ECD-classification, the (L)KDPI was significantly associated with risk of graft loss (hazard ratio per 10% increase in (L)KDPI: 1.185, 95% confidence interval: 1.033–1.360, p = 0.025). Survival analysis revealed decreased death censored (p < 0.001) and non-death censored (p < 0.001) graft survival in kidneys with an increasing (L)KDPI divided into groups of <35, 35–85, and >85%, respectively.

Conclusion

With a higher granularity compared to the SCD/ECD-classification the (L)KDPI is a promising tool to judge graft quality. The correlation with chronic and acute histological lesions in post-reperfusion kidney biopsies underlines the descriptive value of the (L)KDPI. However, its prognostic value is limited and underlines the urgent need for a more precise prognostic tool adopted to European kidney transplant conditions.
Parents’ and Childcare Workers’ Perspectives Toward SARS-CoV-2 Test and Surveillance Protocols in Pre-school Children Day Care Centers: A Qualitative Study Within the German Wü-KiTa-CoV Project
Authors: Gierszewski David , Kurotschka Peter Konstantin , Krauthausen Maike , Fröhlich Willi , Forster Johannes , Pietsch Franziska , Streng Andrea , Rücker Viktoria , Wallstabe Julia , Hartmann Katrin , Jans Thomas , Engels Geraldine , Romanos Marcel , Heuschmann Peter , Härtel Christoph , Kurzai Oliver , Liese Johannes , Gágyor Ildikó
Published: April 2022

Background

Feasibility of surveillance through continuous SARS-CoV-2 testing in pre-school children and childcare workers (CCWs) to prevent closure of day care centers (DCCs) was proven in the Wü-KiTa-CoV study. The purpose of this study was to describe the factors that facilitate or hinder the implementation of continuous SARS-CoV-2 testing from the perspective of parents and CCWs involved in the study.

Methods

A total of 148 semi–structured telephone interviews, repeated before and after the implementation of the surveillance protocols, were conducted with parents and CCWs belonging to the DCCs involved in Wü-KiTa-CoV and analyzed using qualitative content analysis.

Results

Five main topical categories that influences implementation of surveillance protocols for SARS-CoV-2 in DCCs emerged: Generating valuable knowledge, Impact on daily life, Communication and information, Children’s wellbeing and the Sense of security. Smooth integration in daily routines, quickly delivered test results, and efficient communication and information between the study team and the participants were identified as factors that had a positive impact on implementation. To ensure children’s wellbeing, the introduction of non-invasive testing procedures such as saliva testing, parental involvement to motivate, and prepare children for the procedure, the creation of a child-friendly environment for testing, and use of child-friendly explanations were considered critical. The surveillance was found to increase the sense of security during the pandemic. Conversely, reliability of tests in the surveillance protocols, low participation rates, non-transparent communication, the need to travel to testing sites, fear of quarantine in case of positive test results, concerns about higher workloads, the fear of unpleasant feelings for children, their young age, and changing test teams were considered as hindering factors.

Conclusion

This qualitative study of parents of children in day care and DCC staff under surveillance through continuous testing for SARS-CoV-2 in nine German DCCs identified several factors that facilitate or hinder its implementation. These should be considered when planning screening interventions to prevent the spread of SARS-CoV-2 or other infectious diseases in pre-school children DCCs.
A Surface Exposed, Two-Domain Lipoprotein Cargo of a Type XI Secretion System Promotes Colonization of Host Intestinal Epithelia Expressing Glycans
Authors: Grossman Alex S. , Escobar Cristian A. , Mans Erin J. , Mucci Nicholas C. , Mauer Terra J. , Jones Katarina A. , Moore Cameron C. , Abraham Paul E. , Hettich Robert L. , Schneider Liesel , Campagna Shawn R. , Forest Katrina T. , Goodrich-Blair Heidi
Published: April 2022

The only known required component of the newly described Type XI secretion system (TXISS) is an outer membrane protein (OMP) of the DUF560 family. TXISSOMPs are broadly distributed across proteobacteria, but properties of the cargo proteins they secrete are largely unexplored. We report biophysical, histochemical, and phenotypic evidence that Xenorhabdus nematophila NilC is surface exposed. Biophysical data and structure predictions indicate that NilC is a two-domain protein with a C-terminal, 8-stranded β-barrel. This structure has been noted as a common feature of TXISS effectors and may be important for interactions with the TXISSOMP. The NilC N-terminal domain is more enigmatic, but our results indicate it is ordered and forms a β-sheet structure, and bioinformatics suggest structural similarities to carbohydrate-binding proteins. X. nematophila NilC and its presumptive TXISSOMP partner NilB are required for colonizing the anterior intestine of Steinernema carpocapsae nematodes: the receptacle of free-living, infective juveniles and the anterior intestinal cecum (AIC) in juveniles and adults. We show that, in adult nematodes, the AIC expresses a Wheat Germ Agglutinin (WGA)-reactive material, indicating the presence of N-acetylglucosamine or N-acetylneuraminic acid sugars on the AIC surface. A role for this material in colonization is supported by the fact that exogenous addition of WGA can inhibit AIC colonization by X. nematophila. Conversely, the addition of exogenous purified NilC increases the frequency with which X. nematophila is observed at the AIC, demonstrating that abundant extracellular NilC can enhance colonization. NilC may facilitate X. nematophila adherence to the nematode intestinal surface by binding to host glycans, it might support X. nematophila nutrition by cleaving sugars from the host surface, or it might help protect X. nematophila from nematode host immunity. Proteomic and metabolomic analyses of wild type X. nematophila compared to those lacking nilB and nilC revealed differences in cell wall and secreted polysaccharide metabolic pathways. Additionally, purified NilC is capable of binding peptidoglycan, suggesting that periplasmic NilC may interact with the bacterial cell wall. Overall, these findings support a model that NilB-regulated surface exposure of NilC mediates interactions between X. nematophila and host surface glycans during colonization. This is a previously unknown function for a TXISS.
Suppression of Aspergillus fumigatus Germination by Neutrophils Is Enhanced by Endothelial-Derived CSF3 Production
Authors: Zhang Wenxin , He Dan , Wei Yunyun , Shang Shumi , Li Dong , Wang Li
Published: April 2022

Infection with Aspergillus fumigatus can cause life-threatening diseases in immunocompromised patients with an unacceptable mortality rate. Angioinvasion is one of the features of severe invasive aspergillosis. Neutrophils are short-lived immune cells regulated by colony-stimulating factor 3 (CSF3) that play a key role in anti-fungal immune responses. To investigate the interactions between A. fumigatus and the host immune cells, such as neutrophils, we stimulated human umbilical vein endothelial cells (HUVECs) with the conidia of A. fumigatus, and co-cultured them with human neutrophils. Apoptosis and functions of neutrophils were analyzed. Our results showed that HUVECs upregulate the expression of CSF3, which could reduce the apoptosis of neutrophils while enhancing their functions. Lack of CSF3 was associated with enhanced apoptosis of neutrophils with impaired function. This work indicated that the CSF3 is required for neutrophil survival and function, at least in the early stages of A. fumigatus infection.
Mutational Pattern Induced by 5-Fluorouracil and Oxaliplatin in the Gut Microbiome
Authors: Wan Li , Li Hexin , Sun Gaoyuan , Zhang Lili , Xu Hongtao , Su Fei , He Shunmin , Xiao Fei
Published: April 2022

Chemotherapeutic agents, such as 5-fluorouracil (5-FU) and oxaliplatin (Oxi), can not only kill the cancer cell but also influence the proliferation of gut microbiota; however, the interaction between these drugs and gut microbiota remains poorly understood. In this study, we developed a powerful framework for taxonomy composition and genomic variation analysis to investigate the mutagenesis effect and proliferation influence of chemotherapeutic agents, such as 5-FU and Oxi, on gut microbiota and the interaction between these drugs and gut microbiota during chemotherapy. Using the gut microbiome data, we detected 1.45 million variations among the chemotherapy groups and found the drugs significantly affected mutation signatures of gut microbiota. Oxi notably increased transversion rate, whereas 5-FU reduced the rate. Traits related to cell division and nutrient mobilization showed evidence of strong selection pressure from chemotherapeutic agents. In addition, drug-associated bacteriome shift patterns and functional alterations were found: the metabolism changes in the 5-FU group implied that gut microbiota could provide additional nicotinamide adenine dinucleotide (NAD+) to inhibit cancer cell autophagy; in the Oxi group, the ribosome and lysine biosynthesis genes were obviously enriched. Our study provides a blueprint for characterizing the role of microbes and drug–microbe interaction in the gut microbiota response to chemotherapy.
Analysis of Phyllosphere Microorganisms and Potential Pathogens of Tobacco Leaves
Authors: Xiang Li-Gang , Wang Han-Cheng , Wang Feng , Cai Liu-Ti , Li Wen-Hong , Hsiang Tom , Yu Zhi-He
Published: April 2022

In the tobacco phyllosphere, some of the microbes may have detrimental effects on plant health, while many may be neutral or even beneficial. Some cannot be cultivated, so culture-independent methods are needed to explore microbial diversity. In this study, both metagenetic analysis and traditional culture-dependent methods were used on asymptomatic healthy leaves and symptomatic diseased leaves of tobacco plants. In the culture-independent analysis, asymptomatic leaves had higher microbial diversity and richness than symptomatic leaves. Both asymptomatic and symptomatic leaves contained several potentially pathogenic bacterial and fungal genera. The putative bacterial pathogens, such as species of Pseudomonas, Pantoea, or Ralstonia, and putative fungal pathogens, such as species of Phoma, Cladosporium, Alternaria, Fusarium, Corynespora, and Epicoccum, had a higher relative abundance in symptomatic leaves than asymptomatic leaves. FUNGuild analysis indicated that the foliar fungal community also included endophytes, saprotrophs, epiphytes, parasites, and endosymbionts. PICRUSt analysis showed that the dominant functions of the bacterial community in a symptomatic leaf were cellular processes and environmental information processing. In the other five foliar samples, the dominant functions of the bacterial community were genetic information processing, metabolism, and organismal systems. In the traditional culture-dependent method, 47 fungal strains were isolated from 60 symptomatic tobacco leaf fragments bearing leaf spots. Among them, 21 strains of Colletotrichum (29%), Xylariaceae (14%), Corynespora (14%), Pestalotiopsis (10%), Alternaria (10%), Epicoccum (10%), Byssosphaeria (5%), Phoma (5%), and Diaporthe (5%) all fulfilled Koch’s postulates and were found to cause disease on detached tobacco leaves in artificial inoculation tests. Symptoms on detached leaves caused by three strains of Corynespora cassiicola in artificial inoculation tests were similar to the original disease symptoms in the tobacco field. This study showed that the combined application of culture-dependent and independent methods could give comprehensive insights into microbial composition that each method alone did not reveal.
Effects of Replacing Alfalfa Hay With Native Grass Hay in Pelleted Total Mixed Ration on Physicochemical Parameters, Fatty Acid Profile, and Rumen Microbiota in Lamb
Authors: Du Shuai , You Sihan , Sun Lin , Wang Xiaolong , Jia Yushan , Zhou Yulei
Published: April 2022

This study aimed to investigate the effects of replacing alfalfa with native grass on growth performance, meat quality, and rumen microbiota of lambs. Forty-five 6-month-old Ujimqin lambs with live body weight (BW) of 29.50 ± 0.26 kg were used for the experiment, and the lambs were assigned to three diet treatments (three pens per treatment and five lambs per pen) with 30 square meters per pen in semi-open housing based on similar BW. The lambs have received 30% alfalfa (HA, high alfalfa percentage group), 20% alfalfa (MA, moderate alfalfa percentage group), and 10% alfalfa (LA, low alfalfa percentage group) diets, respectively (dry matter basis). The diet treatments had a significant (P < 0.05) influence on the dry matter intake of lambs and the dry matter intake increased with the increasing percentages of native grass hay, while the significantly (P < 0.05) lower final BW and average daily gain were observed in the MA and LA groups compared with that in the HA group. The diet had a significant (P < 0.05) difference in meat pH value, water loss rate, cooked meat rate, moisture, and intramuscular fat, while no significant (P > 0.05) difference was found in protein. As native grass hay percentages increased in the diet, the contents of palmitic (C16:0) and palmitoleic (C16:1 cis-9) in the HA and MA groups were significantly (P < 0.05) lower than that in the LA groups, and compared with the HA group, the contents of elaidic (C18:1 trans-9), oleic (C18:1 cis-9), and linoleic (C18:2 cis-9–cis-12) were significantly (P < 0.05) increased in the MA and LA groups. The content of α-linolenic (C18:3n3) was significantly (P < 0.05) higher in the LA group than that in the HA and MA groups. The principal coordinate analysis profile displayed that the composition of the bacterial community of these groups was distinctly separated from each other. No significant (P > 0.05) difference was observed in the dominant rumen bacteria at the phyla and genus levels. In conclusion, the meat quality and fatty acid profile analysis revealed that replacing alfalfa hay with native grass hay is more beneficial for Mongolian lambs, and the meat from LA diet treatment is better than the others. In addition, correlation analysis of the association of rumen microbiome with growth performance, meat quality, and fatty acid profile provides us with a comprehensive understanding of the composition and function of rumen microbiota. These findings could provide knowledge of how the diet affects the animal performance, meat quality of lambs, and microbiota of the rumen, laying a theoretical basis for replacing alfalfa with native grass.
Emergence of a Hypervirulent Tigecycline-Resistant Klebsiella pneumoniae Strain Co-producing blaNDM–1 and blaKPC–2 With an Uncommon Sequence Type ST464 in Southwestern China
Authors: Hao Jingchen , Zhang Bangqin , Deng Jiamin , Wei Yueshuai , Xiao Xue , Liu Jinbo
Published: April 2022

Emergence of blaNDM–1 and blaKPC–2 co-producing Klebsiella pneumoniae strains is currently attracting widespread attention, but little information is available about their tigecycline resistance, virulence, and prevalence in Southwest China. In July 2021, an extensively drug-resistant K. pneumoniae strain AHSWKP25 whose genome contained both blaNDM–1 and blaKPC–2 genes was isolated from the blood of a patient with the malignant hematological disease in Luzhou, China. We investigated the resistance profiles of AHSWKP25 using microbroth dilution, agar dilution, modified carbapenemase inactivation (mCIM), and EDTA-modified carbapenemase inactivation methods (eCIM). The virulence of AHSWKP25 was assessed through string tests, serum killing assays, and a Galleria mellonella larval infection model. Conjugation and plasmid stability experiments were conducted to determine the horizontal transfer capacity of plasmids. And efflux pump phenotype test and real-time quantitative reverse transcription-PCR (RT-PCR) were used to determine its efflux pump activity. Sequencing of AHSWKP25 determined that AHSWKP25 belonged to ST464, which is resistant to antibiotics such as carbapenems, tetracycline, fluoroquinolones, tigecycline, and fosfomycin. The efflux pump phenotype tests and RT-PCR results demonstrated that efflux pumps were overexpressed in the AHSWKP25, which promoted the tigecycline resistance of the bacteria. AHSWKP25 also showed hypervirulence and serum resistance in vitro model. AHSWKP25 carried several different plasmids that contained blaNDM–1, blaKPC–2, and mutated tet(A) genes. Sequence alignment revealed that the plasmids carrying blaNDM–1 and blaKPC–2 underwent recombination and insertion events, respectively. We demonstrated that an X3 plasmid carrying blaNDM–1 was transferred from pSW25NDM1 to E. coli J53. We also identified missense mutations in the ramR, rcsA, lon, and csrD genes of AHSWKP25. Our results highlighted the potential of blaNDM–1 and blaKPC–2 co-producing K. pneumoniae strains to further develop antimicrobial resistance and hypervirulent phenotypes, but measures should be taken to closely monitor and control the spread of superbugs with multidrug-resistant phenotypes and hypervirulence.