bluuebunny/crossref_metadata_2025 huggingface dataset:
Peran Pemerintah Dalam Mengembangkan Obyek Wisata Goa Kreo
Authors: Tutik Tutik
Published: October 2022

Obyek Wisata bertujuan untuk meningkatkan potensi pariwisata dan meningkatkan potensi masyarakat untuk menciptakan kemandirian ekonomi melalui sektor pariwisata. Goa Kreo merupakan salah satu obyek wisata yang memiliki peran dalam sosial ekonomi masyarakat disekitarnya. Namun dengan perkembangan teknologi yang mulai merambah saat ini memiliki dampak bagi perkembangan Goa Kreo, tak terkecuali bagi kehidupan ekonomi masyarakat sekitar. Penelitian ini bertujuan untuk menganalisis peran pemerintah dan masyarakat dalam mengelola Obyek Wisata Goa Kreo.  Metode pengambilan data dalam penelitian ini adalah dengan teknik kuesioner dan wawancara. Jenis penelitian ini adalah kualitatif dengan metode analisis swot. Hasil deskriptif kualitatif menunjukkan profil dan kondisi Obyek Wisata Goa Kreo, sedangkan hasil perhitungan analisis swot menunjukkan bahwa Goa Kreo berada pada kuadran I, memiliki kekuatan dan peluang yang dominan sehingga berpotensi untuk dikembangkan.
PELATIHAN PENGEMBANGAN POTENSI DESA WISATA BERBASIS EKOWISATA DAN PENGETAHUAN GUIDE DI PATEMON GUNUNGPATI SEMARANG
Authors: Suwarti  , Dyah Palupiningtyas
Published: October 2022

Development of Village Potential in tourism in Patemon Village, Gunungpati District, Semarang, as well as the implementation of community service by several lecturers who have knowledge and skills about tourism. This activity was in the form of socialization about the Potential of Tourism Village based on Ekowista and Guide. The main objective of this activity is to help the people of Patemon Village, Gunungpati District, Semarang City, in order to develop the potential of their village for tourism activities. As well as providing benefits in the form of Training and Skills related to the Tourism Sector based on the Tourism Law Number 10 of 2009 in Chapter I article 1. The method used is Participatory Action Research (PAR) Analyzing the potential of Tourism Villages, in collaboration with UPRRID KKN Students, village potential based on maps, thus the principle of developing village potential as a tourist destination location through FGD of Village potential for the development of Leading Ecotourism. With the principle of making tour packages by practicing Guide training, this is the concept of Tourism Promotion and its marketing
PEMANFAATAN LINGKUNGAN RUMAH MELALUI PELATIHAN PEMBUATAN BIOPORI DI KELURAHAN PURWOYOSO KECAMATAN NGALIYAN KOTA SEMARANG
Authors: Nuryanti  , Zusrotin  , Eko Heri Widiastuti  , R.Soelistijanto
Published: October 2022

Community service with counseling steps carried out by the Extension Team of the Department of History Education, Faculty of Teacher Training and Education, Ivet University in Purwoyoso Village, Ngaliyan District, Semarang City, has succeeded in increasing public awareness of the importance of a healthy environment and making absorption through Biopori. Human life, cannot be separated from the environment around the house where you live. Both the natural environment and the social environment. We breathe requires air from the environment. Eating, drinking, keeping healthy, everything involves the environment. From the environment, humans can take advantage of parts of the environment such as animals, plants, water, air, sunlight, salt, wood, mining products and so on for their daily needs. But it is not only humans who live like that. Other living things such as animals and microbial animals and plants can also live because of their environment. A healthy environment in the form of shady plants and trees can be a source of oxygen and become a shelter for residents. Apart from being a shelter for residents, it is also a place for residents to chat and play with children. The role of Biopores is also important, as water infiltration when it rains so that it can provide water for the plants around it, so that the plants around it become fertile.
UPAYA PEMENUHAN GIZI TAMBAHAN PADA ANAK MELALUI PELATIHAN PEMBUATAN NUGET AYAM KELOR DAN STICK DAUN KELOR DI DUSUN PANGENYAR KECAMATAN SUMBAWA
Authors: Ana Merdekawaty  , Fatmawati  , Ardiyansyah
Published: October 2022

The purpose of this service activity is to increase the knowledge and skills of participants in Moringa processing; increase participants' knowledge about the potential of Moringa leaves as a healthy food ingredient, and produce Moringa chicken nuget products and Moringa leaf sticks. The activity method used is mentoring with activity stages; 1. preparation, 2. implementation, 3. evaluation. The results of the activity showed an increase in the knowledge and skills of the participants in processing Moringa leaves, participants knew that Moringa leaves have good nutritional potential and can be developed as a healthy food processing ingredient, and participants are skilled in processing Moringa leaves into Moringa chicken nuggets and Moringa leaf sticks. . Given the large benefits of this activity, it is advisable to carry out further activities in the form of assistance in marketing Moringa leaf processed products in order to increase people's income.
PEMBERDAYAAN MASYARAKAT DALAM PENERAPAN CHS DI DESA SIKASUR KABUPATEN PEMALANG DI ERA NEW NORMAL
Authors: Julian Andriani Putri  , Wenefrida Ardhian Ayu Hardiani
Published: October 2022

The Covid-19 pandemic has had a significant impact on all industrial sectors in Indonesia, and one of them is the country's tourism sector since the beginning of

2020 and consequently has had a tremendous impact on tourism villages in Indonesia. A survey conducted by the Desa Wisata Institute (2020) of 97 tourist villages throughout Indonesia noted that no less than 92.8% of villages (or around

 

 

 

 

 

population in these villages continued to maintain their profession. the original. It is  undeniable  that tourism  contributes  a large amount  of  income  so  that  this pandemic has caused the tourist villages to suffer losses of tens to hundreds of millions of rupiah. Nearly 99% of tourism villages in Indonesia were closed due to the implementation of social restriction policies which resulted in the cancellation of visiting guests and in the end it had a long-term effect on tourism entrepreneurs in tourist villages such as restaurant entrepreneurs, homestay owners, craftsmen, artists. For this reason, in order to help revive the existence of the Tourism Village in the community, in the community service of STIEPARI Semarang this time the goal is in Sikasur Village, Pemalang Regency, this program was held with the aim of helping tourist villages raise their ranks and providing a forum for community service for the academics involved. CHS (Cleanliness, Health, Safety) which in the new normal era is considered very important to be implemented in various tourist destinationsThese community service activities include location surveys, Forum Group Discussion (FGD), and outreach. The results of this activity are expected to make Sikasur Tourism Village begin to adapt and develop in this new normal era
PENERAPAN METODE DAN APLIKASI STATISTIK (SPSS dan Excel) UNTUK MENINGKATKAN MOTIVASI GURU DALAM PENELITIAN DAN PUBLIKASI ILMIAH
Authors: M. Y. Matdoan  , M. W. Talakua  , N. Lewaherilla  , S. J. Latupeirissa  , J. E. T. Radjabaycolle
Published: October 2022

The development of computers in the current era can help teachers improve human resources (HR), especially in the field of education. SPSS and Excel are statistical applications that can process teacher research data such as education management data and classroom action research data and so on. This community service (PKM) is carried out in the form of statistical application training (SPSS and Excel) as well as writing techniques and publication of scientific articles. Participants in this activity consisted of 21 teachers and employees of the Muhammadiyah Mamala High School in Central Maluku. The conclusion obtained in this activity is that activities that have been carried out in the form of training are considered very good (99%). All material that has been planned can be delivered, although due to time constraints there are some materials that are only presented in an outline. The participants' ability in mastering the material was considered very

 

1Jurusan Matematika FMIPA Universitas Pattimura, Ambon-Maluku

 

 

 

 

good (98%). This is based on the teacher's ability in training and trials in the form of classroom action research data processing using Excel and SPSS applications. In addition, from the results of the training given to the participants, it was found that all the training participants perfectly mastered the tasks given in the training.
PENGARUH LOKASI DAN KUALITAS PELAYANAN TERHADAP KEPUASAN TAMU DI LIND’S ICE CREAM DAN RESTO PAPANDAYAN SEMARANG
Authors: Andri Yanusman Amazihono
Published: October 2022

This research was conducted at Lind's Ice Cream and Resto Papandayan Semarang which aims to analyze and explain the influence of location and service quality on guest satisfaction, as well as to determine which variables have the most dominant influence on guest satisfaction. The method used in this research is a survey with quantitative analysis,. The number of samples in this study were 86 respondents who visited Lind's Ice Cream and Resto Papandayan Semarang. Based on the results of SPSS calculations, the hypothesis at the tested level is significant and has a positive effect on the regression equation,And it is proven that the service quality  variable  has  the  most  dominant  effect   on  guest   satisfaction  with  a regression coefficient of 0.794. The conclusion of this study  is that The quality of service that has the most positive effect on guest  satisfaction at Lind's Ice Cream and Resto Papandayan Semarang.
POTENSI USAHA TOURISM WEB SERVICE WWW.AYOHOLIDAY.COM KAISA GROUP SEMARANG
Authors: Ester Wulandari
Published: October 2022

Scientific study activities on tourism, mice, and marketing communications. The activities carried out are in the form of participating in discussions and assigned to attend seminars, aiming to enrich knowledge about tourism, mice and marketing communications. This activity is a practice to process vendor data that will collaborate with the office into one in the service provider partner file. Both transportation, accommodation, restaurants, logistics and human resources. Aims to collect data on cooperation partners so that the data is neat and can be searched at any time when needed
MODEL PENGEMBANGAN POTENSI AGROWISATA DI PT CENGKEH ZANZIBAR (PLANTERA) PATEAN KENDAL
Authors: Nina Mistriani  , RudiPrasetyoArid
Published: October 2022

This community service activity at Plantera Patean Kendal Agro-tourism is carried out in order to fulfill the request of Plantera Patean Kendal Agro-tourism (PT Cengkeh Zanzibar). As well as the implementation of community service by several lecturers who have knowledge and skills about tourism. This activity was in the form of an analysis FGD on the packaging of agro-based tourism products based on market segmentation. The main objective of this activity is to assist Plantera Agro-tourism in developing agro-tourism potential. As well as providing benefits in the form of training and skills related to the tourism sector.
Efektivitas Pembelajaran Daring Menggunakan Zoom Terhadap Hasil Belajar Mahasiswa Pada Mata Kuliah Matematika Diskrit
Authors: Kurnia Ulfa Isni , Alfina Alfina , Kurniawansah Alfi
Published: October 2022

Pemanfaatan aplikasi zoom pada pembelajaran di prodi Pendidikan Teknologi Informasi dilaksanakan dalam menunjang pembelajaran secara daring saat pademi. Tujuan dari penelitian ini adalah mengungkapkan keefektifan pembelajaran daring menggunakan zoom dalam pemahaman mahasiswa terhadap materi pembelajaran pada mata kuiah matematika diskrit. Teknik pengumpulan data menggunakan instrumen pretest dan posttest. Teknik analisis data menggunakan teknik analisis data kuantitatif untuk melihat signifikansi dan keefektifan yaitu Paired Sample t Test dan Gain Score. Pembelajran daring menggunakan zoom ternyata tidak terbukti efektif di gunakan pada mata kuliah matematika diskrit tetapi Pembelajaran tatap muka secara langsung yang dapat meningkatkan hasil belajar dan pemahaman mahasiswa. Hal ini dilihat dari pretes dan posstes mahasiswa. Penelitian ini juga dilakukan analisis signifikansi yang lebih kecil dari taraf signifikansi (P-Value < 0,05) yaitu P-Value = 0,041, yang berarti bahwa ada perbedaan yang signifikansi antara menggunakan aplikasi zoom dan tatap muka secara langsung di kelas, dimana hasil normalized gain diperoleh nilai yaitu 0,64.
Pengembangan Media Permainan Smart Jenga dalam Meningkatkan Kemampuan Kognitif Anak Kelompok B TK Pembina Ampenan Tahun 2022
Authors: Azizah Wanda , Suarta I Nyoman , Astini Baik Nilawati , Astawa I Made Suwasa
Published: October 2022

Bermain merupakan hak dasar bagi anak usia dini, karena dengan bermain seluruh aspek perkembangan anak dapat terstimulasi dengan cara yang menyenangkan. Penelitian ini dilatarbelakangi oleh kurangnya penggunaan media permainan untuk menstimulasi kemampuan kognitif anak secara maksimal. Tujuan dari penelitian ini adalah untuk mengembangkan media permainan smart jenga guna meningkatkan kemampuan kognitif anak. Untuk mencapai tujuan penelitian digunakan metode pengumpulan data berupa observasi dan dokumentasi dengan teknik analisis kualitatif deskriptif. Hasil dari penelitian ini adalah smart jenga yang terdiri dari balok jenga, dadu, dan jenga card dengan penerapan menggunakan tahapan permainan yang terdiri dari persiapan permainan yakni menyiapkan alat dan bahan, kelompok, kesepakatan bermain, dan urutan main; kegiatan main yakni anak mengelompokkan balok dan kartu, mengambil balok, menyelesaikan instruksi kartu; dan akhir permainan yakni menghitung skor dan menentukan pemenang, dapat meningkatkan kemampuan kognitif anak. Hal ini dibuktikan dengan hasil persentase yang diperoleh yaitu 47,16% pada tahap pengembangan I menjadi 74,75% pada tahap pengembangan II dan pada tahap pengambangan III capaian kognitif siswa memenuhi keritiria berkembang sangat baik dengan nilai rata-rata yaitu 88,33%. Dengan demikian dapat  disimpulkan bahwa pengembangan  smart jenga dapat meningkatkan kemampuan kognitif anak kelompok B TK Negeri Pembina Ampenan dan hasil penelitian ini dapat dmanfaatkan dalam menstimulasi perkembangan kognitif anak.
The Syllabus Evaluation: Our Discovery Island 5–Student Book published by Pearson Education
Authors: Sitoresmi Woro Endah , Damayanti Nina Afria
Published: October 2022

The success of English language subject is hardly required because the prior of language learning is communication. However, the success itself is not only based on the teacher, method, curriculum but also the package such as syllabus and textbook for delivering materials. Syllabus and textbook refer to the content of what is to be taught and the skills to be mastered. Furthermore, both of them help teacher to provide the materials well and to be relevant into the present context. The goal of this paper is to analyze deeply the English language syllabus design in Our Discovery Island 5–student book published by Pearson Education that is used by the elementary students of grade V in Citra Indonesia school, Bintaro. This study belongs to descriptive qualitative study. The findings show that the syllabus design in this book used the linear format to display the contents which include scope, sequence, integration, articulation and balance. Meanwhile for the syllabus model, it used process model on subject center design.
La estrategia de los 50 municipios prioritarios en materia de seguridad pública
Authors: Vizcaíno Zamora Álvaro
Published: October 2022

La estrategia del Gobierno federal de México consiste en concentrar acciones de prevención y persecución de delitos en los 50 municipios que suman el mayor número de homicidios dolosos y no ha dado resultados. La lógica haría pensar que los municipios con mayor población concentran el mayor número de delitos. Sin embargo, hay ciudades que no están entre las 50 más pobladas y presentan muchos homicidios, mientras que otras ciudades con población superior a 500 000 habitantes no están en la lista. Analizar estos extremos muestra la importancia de impulsar la seguridad pública desde lo local.
Factors Increasing Teachers` Motivation: The Case of Vilnius City Municipality
Authors: Stasiukynas Andrius , Žibėnienė Gintautė , Gudelis Dangis , Žemaitaitytė Irena
Published: October 2022

The article presents the results of a quantitative study. The aim of the article is to identify the factors motivating teachers of the Vilnius City Municipality to work. The questionnaire survey of Vilnius city teachers was conducted in April-May, 2020, using the electronic survey tool Qualtrics. Employees of the Vilnius City Municipality Administration distributed the link to the questionnaire to all teachers of the Vilnius city schools by e-mail. 1660 respondents started the questionnaire on this electronic platform, but after eliminating the incomplete questionnaires, a database of 873 respondents who answered all the questions of the questionnaire was created. The survey of teachers of Vilnius city educational institutions has shown that teachers are most motivated by the improvement of teachers' working conditions at school and the reduction of direct control as well as by personal recognition and ensuring favourable opportunities for professional development.
Degradation of Forest Reserves in Asunafo Forest District, Ghana
Authors: Peprah Kenneth , Aabeyir Raymond , Kuuwaabong Gervase
Published: October 2022

Forest reserve degradation is a global concern because it is a storage facility of global biodiversity. In addition, forest reserves contain the wealth of several poor countries, particularly in Africa. Such is the situation in Ghana, which possesses portions of the tropical African rainforest. The timber species thereof has been harvested to create wealth since the 1800s. The wealth of the soils for cocoa production was realised in the first decade of the nineteenth century in Asunafo. Hence, the desire to reserve portions of the forest as protected areas began in 1910. Therefore, the aim of this study is an investigation of the degradation of the forest reserves of Asunafo. The methods of the study include a survey of farmers, key informant interviews, community meetings, and transect drives. The results reveal a progressive increase in the human population, expansion of settlements, and a drastic reduction in the forest reserves by –24.59%. The timber industry, cocoa farming, and population increase have caused forest reserve degradation, a loss of wildlife habitats, an out-migration of elephants, buffalos, and chimpanzees, a loss of plant biodiversity, and an invasion by weeds. The Government of Ghana should increase efforts to halt forest reserve degradation.
Application of a Fully Automated Dried Blood Spot Method for Therapeutic Drug Monitoring of Immunosuppressants: Another Step Toward Implementation of Dried Blood Spot Analysis
Authors: Deprez Sigrid , Stove Christophe
Published: October 2022

Context.—

The follow-up of patients under lifelong immunosuppressant therapy is pivotal to prevent allograft rejection after transplant. Part of the difficulties associated with routine monitoring of immunosuppressant concentrations can be alleviated by home sampling using dried blood spots (DBSs).

Objective.—

To evaluate the applicability of a DBS method for the determination of immunosuppressants in venous blood samples, making use of an automated extraction platform.

Design.—

Paired venous DBSs and whole blood samples were analyzed for tacrolimus (n = 162), sirolimus (n = 47), everolimus (n = 45), and cyclosporin A (n = 61) with liquid chromatography coupled to tandem mass spectrometry, using fully automated extraction for DBSs. Agreement between the automated DBS and whole blood method was assessed by using Bland-Altman comparison. Both an analytical and a clinical acceptance limit were predefined at more than 67% of all paired samples within 20% of the mean of both samples and more than 80% of all paired samples within 20% of the whole blood concentration, respectively.

Results.—

An impact of the hematocrit (hct) on DBS quantitation was observed for all analytes, which could be alleviated for all analytes by using a hct conversion formula based on a tacrolimus data subset: [DBScorrected] = [DBSmeasured]/(1.6305 – 1.559*hct). After correction, both analytical and clinical acceptance criteria were met for all analytes.

Conclusions.—

Automated DBS analysis shows great potential for routine therapeutic drug monitoring of immunosuppressants, avoiding any manual sample handling.
Clinicopathologic Impact of Peptide Hormonal Expression in Rectal Neuroendocrine Tumors
Authors: Kim Jisup , Yang Dong-Hoon , Jung HaeSung , Cho HyungJun , Jang Hyeung-Jin , Yoo Changhoon , Park In Ja , Ryoo Baek-Yeol , Ryu Jin-Sook , Hong Seung-Mo
Published: October 2022

Context.—

Although several neuroendocrine cell types constitute gastroenteropancreatic neuroendocrine tumors (NETs), the clinical and prognostic implications of the expression of multiple peptide hormones have not been comprehensively evaluated in rectal NETs.

Objective.—

To identify the clinicopathologic characteristics and prognostic impact of peptide hormone expression.

Design.—

We evaluated the expression of peptide YY (PYY), glucagon, somatostatin, serotonin, insulin, and gastrin using immunolabeling in 446 endoscopically or surgically resected rectal NETs.

Results.—

PYY, glucagon, serotonin, somatostatin, insulin, and gastrin were expressed in 261 of 389 (67.1%), 205 of 446 (46.0%), 36 of 446 (8.1%), 33 of 446 (7.4%), 2 of 446 (0.4%), and 1 of 446 cases (0.2%), respectively. Immunoreactivity to any peptide hormone was present in 345 of 446 cases (77.4%). Tumors expressing serotonin or somatostatin were associated with lymphovascular invasion, chromogranin A expression, and shorter disease-free survival (DFS). Rectal NETs were classified as L-cell, enterochromaffin-cell, D-cell, null-expression, or mixed-expression type based on peptide hormonal expression status. Patients with D-cell NET had the shortest DFS (10-year DFS, 54.5%), followed by those with enterochromaffin-cell NET (89.5%), null expression (97.0%), L-cell NET (99.6%), and mixed-expression NET (100%; P < .001). Multivariable analyses revealed that somatostatin expression was an independent indicator of poor prognosis with respect to DFS in rectal NETs (P = .001).

Conclusions.—

Somatostatin expression is a poor prognostic indicator in patients with rectal NETs. Therefore, additional peptide hormonal immunolabeling, including somatostatin, serotonin, and PYY, in rectal NETs can provide more information regarding DFS.
Burnout and Disengagement in Pathology: A Prepandemic Survey of Pathologists and Laboratory Professionals
Authors: Smith Stephen M. , Liauw Daniel , Dupee David , Barbieri Andrea L. , Olson Kristine , Parkash Vinita
Published: October 2022

Context.—

Despite widely prevalent burnout and attendant disengagement in medicine, the specific patterns and drivers within pathology and laboratory medicine are uncommonly studied.

Objective.—

To assess the prevalence and drivers of burnout among pathology and laboratory medicine professionals, retrospectively, prior to the COVID-19 pandemic.

Design.—

This was a cross-sectional, mixed-methods study engaging pathology and laboratory medicine professionals as subjects.

Results.—

Of 2363 respondents, 438 identified as pathologists, 111 as pathology assistants, and 911 as pathology and laboratory professionals. The burnout rate was 58.4% (1380 of 2363) across all respondents in pathology and laboratory medicine. Burnout varied by job role (P < .01) and was highest among pathology and laboratory professionals. Disparities in burnout rate were observed by race. Fifty-six percent (1323 of 2363) of respondents felt that they had at least 1 symptom of burnout and were advancing toward a breaking point. Underlying factors ranked highly among all groups included control over workload and loss of meaning in work.

Conclusions.—

Data provided from this cohort may help departments create successful strategies to reduce disengagement and burnout in the laboratory, especially during periods of increased stress as experienced during the COVID-19 pandemic. Further, these data may serve as a baseline comparison for future studies.
Evidence-Based Acquisition at Hacettepe University Libraries
Authors: Yılmaz Damla , Ünal Yurdagül
Published: October 2022

To determine the most suitable acquisition model, or to decide whether or not the model they have already chosen is functioning efficiently and economically, librarians must carry out relevant evaluations of their current or potential acquisition models. In this study, an evaluation of the Cambridge evidence-based acquisition (EBA), was carried out at Hacettepe University Libraries between December 16, 2019, and December 31, 2020. Within the scope of the EBA, the number of e-books opened for access was 35,624, the number of unique books used was 2,462 and the number of the books purchased at the end of the model was 168. In total, the books were used 36,934 times. Ninety-three percent of the books were never used during the EBA model implementation term. While 52 percent of the books opened for access consisted of “books for research,” 47 percent of the number of unique books used consisted of “coursebooks.” Usage cost per unit was calculated as 0.82 USD, and the average book cost was calculated at 180 USD. Purchasing the books based on their list price was a reason for the high average book cost; nevertheless, one should consider that the entire collection of books was open for access for a year-long period of evaluation. In addition, one should not forget that further usage of e-books from the entire collection would decrease the unit cost of the books in the final purchase. During the implementation term, we observed that the Cambridge EBA Model was a suitable option for Hacettepe University Libraries.
Integrated development of Quality and information management systems in organized vehicle fleet: Possibilities and opportunities
Authors: Vasić Miloš , Brzaković Miodrag , Stanojević Nada
Published: October 2022

The subject of this paper is a complementary area consisting of management systems (QMS) and information systems (IS), i.e., the theoretical and practical need for their parallel design, development, implementation and operation. Companies are increasingly concerned with managing the quality of their products and services in accordance with international standards. Designing a quality management system requires the intensive participation of managers and employees who perform analysis and reengineering of business processes and accompanying procedures. Very similar requisites and tasks characterize the design of a company's information system, but these two endeavors are most often implemented as separate projects, handled by different teams, equipped with unrelated methodologies. Within the scope of this paper, the author's ambition was to establish a model of synergistic connection of the information system and the quality management system through the establishment of a Quality management system suitability function and check to what extent the definition of common databases, integrated data model and information model confirms or refutes the hypothesis that the integration of QMS and IS contributes to better overall business results and individually improves each of the connected systems.
Malondialdehyde as an independent predictor of body mass index in adolescent girls
Authors: Klisic Aleksandra , Malenica Maja , Kostadinovic Jelena , Kocic Gordana , Ninic Ana
Published: October 2022

Background: Given the fact that the studies that examined oxidative stress in relation to obesity that included late adolescents are scarce and show inconclusive results we aimed to investigate a wide spectrum of nitro-oxidative stress biomarkers i.e., malondialdehyde (MDA), xanthine oxidase (XO), xanthine oxidoreductase (XOD), xanthine dehydrogenase (XDH), advanced oxidation protein products (AOPP) and nitric oxide products (NOx), as well as an antioxidative enzyme, i.e., catalase (CAT) in relation with obesity in the cohort of adolescent girls ages between 16 and 19 years old. Methods: A total of 59 teenage girls were included in this cross-sectional study. Binary logistic regression analysis was performed to examine possible associations between biochemical and nitro-oxidative stress markers and body mass index (BMI). Results: There were not significant differences between oxidative stress markers between normal weight and overweight/obese girls (i.e., AOPP, XOD, XO, XDH) and CAT, except for MDA (p<0.001) and NOx (p=0.010) concentrations which were significantly higher in overweight/obese adolescent girls. Positive associations were evident between BMI and high sensitivity C-reactive protein (hsCRP) (OR=2.495), BMI and uric acid (OR=1.024) and BMI and MDA (OR=1.062). Multivariable binary regression analysis demonstrated significant independent associations of BMI and hsCRP (OR=2.150) and BMI and MDA (OR=1.105). Even 76.3% of the variation in BMI could be explained with this Model. Conclusions: Inflammation (as measured with hsCRP) and oxidative stress (as determined with MDA) independently correlated with BMI in teenage girls.
Czech Preschool Teachers’ Contrasting Beliefs about Inquiry-based Activitie
Authors: Gavora Peter , Wiegerová Adriana
Published: October 2022

Using a self-reporting measure, the study examined IBA-related beliefs of Czech preschool teachers (n = 1,004). In addition, it explored the beliefs of teachers who strongly agreed (n = 564) and those who strongly disagreed (n = 67) with the implementation of IBA in preschool classes. The findings show that teachers within the full sample, as well as those within the contrasting subsamples, hold considerably strong beliefs about the potential of IBA for children´s cognitive development. At the same time, teachers are aware of the challenges associated with implementing IBA. Teachers who strongly favour IBA have significantly stronger beliefs in the benefits that IBA bring to children and also have stronger beliefs about the knowledge and skills teachers need for IBA planning and implementation than has the group that strongly rejects IBA. In contrast, teachers who strongly reject IBA have stronger beliefs that IBA cause problems in preschool classes. The group of teachers who strongly favour IBA have higher levels of education and fewer years of practice than the contrasting group. They are mostly younger teachers who received a modern professional education and who are more open to newer instructional strategies such as IBA.
Bracon hebetor (Say, 1836) (Hymenoptera: Braconidae)’un bazı biyolojik özelliklerine indol-3-asetik asitin etkisinin deneysel olarak incelenmesi
Authors: AKMAN GÜNDÜZ Nevran Eylem , SAĞLAM Duygu
Published: October 2022

İndole-3-asetik asitin (IAA) parazitoit Bracon hebetor Say (Hymenoptera: Braconidae)’un bazı biyolojik özelliklerine etkileri araştırılmıştır. Farklı konsantrasyonlarda IAA (5, 50, 100, 500 and 1000 mg L-1) konukçu larvaların yapay besinine ilave edilmiştir. Bu konukçularda yetiştirilen parazitoitlerin gelişim süresi, ergin ömür uzunluğu, verim ve eşey oranı IAA konsantrasyonlarına göre değerlendirilmiştir. IAA uygulaması, 50 mg L-1 dışındaki tüm konsantrasyonlarda dişi parazitoitlerin gelişim süresinde kısalmaya neden olmuştur. Erkeklerin gelişim süresi, kontrol grubu ile karşılaştırıldığında 5 and 500 mg L-1 konsantrasyonda önemli bir azalma ve 50 mg L-1 konsantrasyonda dikkate değer bir artış ile IAA uygulanan gruplar arasında dalgalanmalar göstermiştir. IAA B. hebetor’un veriminde olumsuz bir etki yapmıştır. Buna karşın, parazitoitin eşey oranı IAA uygulanan ve uygulanmayan gruplar arasında farklılık göstermemiştir. IAA uygulanmış konukçular üzerinde yetiştirilen B. hebetor dişilerinin ömür uzunluğu 50, 100, 500 ve 1000 mg L-1 konsantrasyonlarında IAA uygulanmamış konukçular üzerindekilere oranla daha kısadır. Erkeklerin ömür uzunluğunda tüm konsantrasyonlarda kontrol grubu ile karşılaştırıldığında önemli bir azalma belirlenmiştir.
Efektivitas Terapi Akupunktur terhadap Penurunan Intensitas Nyeri pada Pasien dengan Nyeri Kanker
Authors: Afladhia Hanna Lianti , Cahyanur Rahmat , Wahdini Sri , Rachman Andhika
Published: October 2022

Latar Belakang: Nyeri merupakan salah satu keluhan paling banyak serta mengganggu kualitas hidup pasien kanker. Beberapa penelitian merekomendasikan intervensi multimodal yang mengkombinasikan antara terapi farmakologis dan nonfarmakologis. Salah satu pilihan tatalaksana nonfarmakologis yang dapat dipertimbangkan adalah terapi akupunktur.Tujuan: Tinjauan kasus berbasis bukti ini disusun untuk mengkaji efektivitas terapi akupunktur terhadap penurunan intensitas nyeri pada pasien dengan nyeri kanker.Metode: Pencarian dilakukan di tiga database (PubMed, Scopus, dan Embase). Kata kunci yang digunakan adalah cancer pain, malignant pain, acupuncture, acupuncture therapy, pain intensity, pain assessment, pain scale. Telaah kritis dilakukan dengan menggunakan panduan dari University of Oxford Centre for Evidence-based Medicine.Hasil: Diperoleh 4 artikel yang relevan dengan pertanyaan klinis. Terapi akupuntur secara konsisten menunjukkan penurunan intensitas nyeri, waktu onset nyeri yang lebih pendek, penurunan dosis dan jumlah analgesik yang dikonsumsi, serta kualitas hidup yang lebih baik dengan efek samping yang minimal, dibandingkan dengan terapi obat saja.Kesimpulan: Terapi akupunktur dapat dipertimbangkan sebagai terapi komplementer dalam penatalaksanaan nyeri kanker, namun tidak untuk menggantikan protokol standar nyeri. Keputusan klinis dalam menerapkan terapi akupunktur pada pasien perlu menilai preferensi pasien, aksesibilitas, serta efektivitas biaya.Kata Kunci: akupunktur, nyeri kanker, intensitas nyeri
The structure-selective endonucleases GEN1 and MUS81 mediate complementary functions in safeguarding the genome of proliferating B lymphocytes
Authors: Fernandez Keith Conrad , Feeney Laura , Smolkin Ryan M , Yen Wei-Feng , Matthews Allysia J , Alread William , Petrini John HJ , Chaudhuri Jayanta
Published: October 2022

During the development of humoral immunity, activated B lymphocytes undergo vigorous proliferative, transcriptional, metabolic, and DNA remodeling activities; hence, their genomes are constantly exposed to an onslaught of genotoxic agents and processes. Branched DNA intermediates generated during replication and recombinational repair pose genomic threats if left unresolved, and so they must be eliminated by structure-selective endonucleases to preserve the integrity of these DNA transactions for the faithful duplication and propagation of genetic information. To investigate the role of two such enzymes, GEN1 and MUS81, in B cell biology, we established B-cell conditional knockout mouse models and found that deletion of GEN1 and MUS81 in early B-cell precursors abrogates the development and maturation of B-lineage cells while the loss of these enzymes in mature B cells inhibits the generation of robust germinal centers. Upon activation, these double-null mature B lymphocytes fail to proliferate and survive while exhibiting transcriptional signatures of p53 signaling, apoptosis, and type I interferon response. Metaphase spreads of these endonuclease-deficient cells show severe and diverse chromosomal abnormalities, including a preponderance of chromosome breaks, consistent with a defect in resolving recombination intermediates. These observations underscore the pivotal roles of GEN1 and MUS81 in safeguarding the genome to ensure the proper development and proliferation of B lymphocytes.
Notch signaling functions in noncanonical juxtacrine manner in platelets to amplify thrombogenicity
Authors: Chaurasia Susheel N , Ekhlak Mohammad , Kushwaha Geeta , Singh Vipin , Mallick Ram L , Dash Debabrata
Published: October 2022

Background:

Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. Existence of functional Notch signaling in enucleate platelets remains elusive.

Methods:

Transcripts/peptides of Notch1 and Delta-like ligand (DLL)–4 were detected in platelets isolated from human blood by RT-qPCR, Western analysis and flow cytometry. Platelet aggregation, granule secretion and platelet-leukocyte interaction were analyzed by lumi-aggregometry and flow cytometry. Platelet-derived extracellular vesicles were documented with Nanoparticle Tracking Analyzer. Platelet thrombus on immobilized collagen was quantified using microfluidics platform. Intracellular calcium was monitored by fluorescence spectrophotometry. Whole blood coagulation was studied by thromboelastography. Ferric chloride-induced mouse mesenteric arteriolar thrombosis was imaged by intravital microscopy.

Results:

We demonstrate expression of Notch1, its ligand DLL-4 and their respective transcripts in human platelets. Synthesis and surface translocation of Notch1 and DLL-4 were upregulated by thrombin. DLL-4, in turn, instigated neighbouring platelets to switch to ‘activated’ phenotype through cleavage of Notch receptor and release of its intracellular domain (NICD), which was averted by inhibition of γ-secretase and phosphatidylinositol-3-kinase (PI3K). Inhibition of Notch signaling, too, restrained agonist-induced platelet activation, and significantly impaired arterial thrombosis in mice. Strikingly, prevention of DLL-4-Notch1 interaction by a blocking antibody abolished platelet aggregation and extracellular vesicle shedding induced by thrombin.

Conclusions:

Our study presents compelling evidence in support of non-canonical juxtacrine Notch signaling within platelet aggregates that synergizes with physiological agonists to generate occlusive intramural thrombi. Thus, Notch pathway can be a potential anti-platelet/anti-thrombotic therapeutic target.

Funding:

Research was supported by grants received by DD from JC Bose Fellowship (JCB/2017/000029), ICMR (71/4/2018-BMS/CAR), DBT (BT/PR-20645/BRB/10/1541/2016) and SERB (EMR/2015/000583). SNC, ME and VS are recipients of ICMR-Scientist-C, CSIR-SRF and UGC-SRF support, respectively. Funders had no role in design, analysis and reporting of study.
A visual sense of number emerges from divisive normalization in a simple center-surround convolutional network
Authors: Park Joonkoo , Huber David E
Published: October 2022

Many species of animals exhibit an intuitive sense of number, suggesting a fundamental neural mechanism for representing numerosity in a visual scene. Recent empirical studies demonstrate that early feedforward visual responses are sensitive to numerosity of a dot array but substantially less so to continuous dimensions orthogonal to numerosity, such as size and spacing of the dots. However, the mechanisms that extract numerosity are unknown. Here, we identified the core neurocomputational principles underlying these effects: (1) center-surround contrast filters; (2) at different spatial scales; with (3) divisive normalization across network units. In an untrained computational model, these principles eliminated sensitivity to size and spacing, making numerosity the main determinant of the neuronal response magnitude. Moreover, a model implementation of these principles explained both well-known and relatively novel illusions of numerosity perception across space and time. This supports the conclusion that the neural structures and feedforward processes that encode numerosity naturally produce visual illusions of numerosity. Taken together, these results identify a set of neurocomputational properties that gives rise to the ubiquity of the number sense in the animal kingdom.
Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA
Authors: Goswami Hemant N , Rai Jay , Das Anuska , Li Hong
Published: October 2022

Cas7-11 is a Type III-E CRISPR Cas effector that confers programmable RNA cleavage and has potential applications in RNA interference. Cas7-11 encodes a single polypeptide containing four Cas7- and one Cas11-like segments that obscures the distinction between the multi-subunit Class 1 and the single-subunit Class-2 CRISPR Cas systems. We report a cryo-EM (cryo-electron microscopy) structure of the active Cas7-11 from Desulfonema ishimotonii (DiCas7-11) that reveals the molecular basis for RNA processing and interference activities. DiCas7-11 arranges its Cas7- and Cas11-like domains in an extended form that resembles the backbone made up by four Cas7 and one Cas11 subunits in the multi-subunit enzymes. Unlike the multi-subunit enzymes, however, the backbone of DiCas7-11 contains evolutionarily different Cas7 and Cas11 domains, giving rise to their unique functionality. The first Cas7-like domain nearly engulfs the last 15 direct repeat nucleotides in processing and recognition of the CRISPR RNA, and its free-standing fragment retains most of the activity. Both the second and the third Cas7-like domains mediate target RNA cleavage in a metal-dependent manner. The structure and mutational data indicate that the long variable insertion to the fourth Cas7 domain has little impact on RNA processing or targeting, suggesting the possibility for engineering a compact and programmable RNA interference tool.
A novel lineage of the Capra genus discovered in the Taurus Mountains of Turkey using ancient genomics
Authors: Daly Kevin G , Arbuckle Benjamin S , Rossi Conor , Mattiangeli Valeria , Lawlor Phoebe A , Mashkour Marjan , Sauer Eberhard , Lesur Joséphine , Atici Levent , Erek Cevdet Merih , Bradley Daniel G
Published: October 2022

Direkli Cave, located in the Taurus Mountains of southern Turkey, was occupied by Late Epipaleolithic hunters-gatherers for the seasonal hunting and processing of game including large numbers of wild goats. We report genomic data from new and publishedCapraspecimens from Direkli Cave and, supplemented with historic genomes from multipleCapraspecies, find a novel lineage best represented by a ~14,000 year old 2.59 X genome sequenced from specimen Direkli4. This newly discoveredCapralineage is a sister clade to the Caucasian tur species (Capra cylindricornisandCapra caucasica), both now limited to the Caucasus region. We identify genomic regions introgressed in domestic goats with high affinity to Direkli4, and find that West Eurasian domestic goats in the past, but not those today, appear enriched for Direkli4-specific alleles at a genome-wide level. This forgotten ‘Taurasian tur’ likely survived Late Pleistocene climatic change in a Taurus Mountain refuge and its genomic fate is unknown.
A/r/tografi som tilgang til udvikling af en sanselig bæredygtighedsdidaktik i kunstfagene
Authors: Illeris Helene , Knudsen Kristian Nødtvedt , Skregelid Lisbet
Published: October 2022

This article presents a study conducted in the spring of 2020 by three researchers from the University of Agder with the aim of exploring and developing possible sensory-based approaches to sustainability in teacher education with a special focus on the arts. Due to the consequences that followed in the wake of Covid-19, the inquiries took an unexpected turn: Digital platforms, isolation and separate borders, became key focal points for the implementation of the project. Using a /r/tography as a methodological frame (Roussel, Cutcher, Cook & Irwin, 2018; Triggs, Irwin & Leggo, 2014; Irwin, 2013), the authors explore how everyday events characterized by affect (Massumi, 2015) and intimacy (Morton, 2018) can form the basis for bodily and sensory approaches to ecological and social sustainability. The empirical material of the study is generated in connection with the authors' implementation of three “propositions”: preparation of a joint meal, joint contact with nature, and running as a joint ritual. The focus was on sensory interaction with each other and with more-than-human actors, including beets, landscapes, bodily routines and not least the digital media. In the concluding part the article analyzes and discusses how the work with the the a/r/tographic propositions led to experiences of risk, loss of control, uncomfortability and vulnerability among the participants, and how these experiences can be related to sensuous experiences in connection to the necessary transition to a more sustainable lifestyle, e.g. experiences of insecurity and loss. Finally the authors propose the areas of attention for future work with sensuous sustainability education in arts teacher education: propositions a a didactic tool, ecological awareness, and sustainability as a sesnsuous form of relating to the world.
Artistic Research in Artists’ Books
Authors: Stana Ingeborg
Published: October 2022

The phenomenon of artistic research attracts considerable attention because of the growing integration between art, design and science. At the same time, it remains a challenging research subject that is interpreted differently by various scientists, designers and artists. This paper presents an attempt to investigate the relationship between artistic research and artistic practice, and the role of artistic research in artists’ books. It aims to present a brief history of artists’ books and show how various artists have captured the complex creative and artistic processes, results or stories behind the works of art.
The article also discusses the problem and potential of representation in artists´ books and examines them as an effective mechanism for disseminating findings of artistic research. To present the multifaceted action behind a work of art implies challenges. The author’s own attempt to capture knowledge from artistic research was challenged while working on a book project and will be part of the discussion. Finally, the study will illustrate how artists’ books contribute to the process of capturing reflections of artistic research and make the manifestations accessible to people outside of the formal context of the lecture halls.
Cover image: Newton, by William Blake (1795). The William Blake Archive, Public Domain