bluuebunny/crossref_metadata_2025 huggingface dataset:
EFEITO DA MÍDIA COLABORATIVA ON-LINE NAS DECISÕES DE RECRUTAMENTO: UM ESTUDO EXPERIMENTAL NO SETOR DE HOSPITALIDADE
Authors: Üngüren Engin , Türker Nazlı
Published: July 2023

RESUMO Esta pesquisa tem como objetivo determinar se os dicionários colaborativos de hipertexto (DCHs) oferecem garantias no âmbito da teoria da garantia. Um roteiro fictício baseado em processos de recrutamento foi usado para examinar se os comentários sobre pessoas em DCHs fornecem uma garantia ou não. O estudo foi conduzido com um desenho pós-teste único com grupo de controle em meio a delineamentos experimentais verdadeiros. A amostra para o estudo foi composta por 359 pessoas, incluindo membros do conselho de administração de estabelecimentos de hospitalidade cinco estrelas com privilégios de recrutamento, subgerentes gerais, responsáveis pela contabilidade e pessoal de recursos humanos. O estudo concluiu que comentários positivos ou negativos sobre candidatos a um emprego em DCHs têm um impacto significativo na perspectiva dos recrutadores em relação aos candidatos e são considerados recursos importantes nos processos de recrutamento para fornecer garantias. Os resultados da pesquisa oferecem uma contribuição prática ao revelar o efeito do conteúdo de mídia social de candidatos na escolha feita pelo recrutador em um processo seletivo.
Salvajes, indígenas, cojas, inválidas: Epistemologías anticapacitistas del Sur
Authors: Pino-Morán Juan Andrés , Rodríguez-Garrido Pía , Lapierre Michelle
Published: July 2023

Resumen El objetivo del manuscrito es presentar una primera aproximación a una propuesta epistemológica que reflexione y se ocupe de la construcción y legitimación de conocimientos generados desde corporeidades abyectas, anormales o tullidas geopolíticamente ubicadas al Sur. Pone especial atención al sistema sexo-género-capacidad en la organización social y epistemológica del saber. En ese devenir, identificamos una posicionalidad y riqueza desperdiciada para el análisis y transformación social regional como resultado de un ordenamiento moderno colonial. De ahí que esta propuesta se inscriba dentro del pensamiento crítico latinoamericano para reflexionar sobre esos lugares otros de enunciación abyecta.
Does Platelet Distribution Width Correlate with Acute Coronary Syndrome Severity and Coronary Thrombus Burden?
Authors: OKUYAN Hızır
Published: July 2023

Aim: Platelets play a pivotal role in the pathogenesis of acute coronary syndrome (ACS), and platelet distribution width (PDW) shows the activities of platelets in circulation. The purpose of this study was to determine the association of PDW with the ACS severity and angiographic intracoronary thrombus burden and Thrombolysis In Myocardial Infarction (TIMI) flow grade.

Materials and Methods: One hundred forty-nine consecutive patients who were diagnosed as having ACS were retrospectively evaluated. Global Registry of Acute Coronary Events (GRACE) scores were calculated from the clinical and laboratory data for ACS severity. TIMI flow grades and thrombus grades of the culprit coronary arteries were calculated for each patient. Patients were grouped into two groups according to their PDW values: high PDW and low PDW. The SPSS 17.0 software was used for statistical analysis.

Results: PDW was found to be correlated with GRACE scores (P
THE EVALUATION OF THE EFFECT OF THE TREATMENTS ON OXIDATIVE STRESS AND INFLAMMATION IN PATIENTS RECEIVING DIFFERENT DIALYSIS MODALITIES
Authors: KOYUNCU Sümeyra , SİPAHİOĞLU Hilal , KOÇYİĞİT İsmail , OYMAK Oktay , TOKGÖZ Bülent , SİPAHİOĞLU Murat
Published: July 2023

ABSTRACT

Aim: To evaluate the effects of different dialysis methods on oxidative stress and inflammation in patients with end-stage renal disease (ESRD) who are newly enrolled in a routine dialysis program.

Material and Methods: In this prospective study, 138 ESRD patients and 30 healthy volunteers were evaluated. Fifty-four of 73 hemodialysis (HD) patients and 51 of 65 periton dialysis (PD) patients completed the study. Other patients were excluded from the study. The levels of superoxide dismutase (SOD) and total antioxidant capacity (TAC) were measured to determine the oxidative stress status, and IL-6, IL-10, and F2 isoprostane levels were measured to determine the inflammation status, just before the start of dialysis treatment and at 6 months.

Results: At the beginning of the study, hs-CRP and IL-6 levels were significantly higher in the patient group compared to the control group (p
PRIMARY EBV INFECTION AND HEMATOLOGICAL FINDINGS IN TURKISH CHILDREN: A RETROSPECTIVE, SINGLE-CENTER STUDY
Authors: ÖNER Nergiz , ŞAHİN Gürses , YEŞİL Şule , FETTAH Ali , ÖZ Fatma Nur , ÇAPKINOĞLU Emre , KILCI Azize , ÜNÜVAR GÖK Şeyma
Published: July 2023

Abstract

Aim: Primary infection with EBV during childhood is usually asymptomatic. Hematologic abnormalities such as hemolytic anemia, thrombocytopenia, and neutropenia are relatively common, but aplastic anemia, severe thrombocytopenia, severe neutropenia, and lymphoid malignancies are rare. In this retrospective study, we have analyzed data from children with primary EBV infection who were examined at our center over 12 years to describe the distribution, clinical features, complications, and outcome of EBV in Turkish children.

Materials and Methods: Data from all children (age
DEPRESSION AND SPIRITUAL WELL-BEING OF HEMODIALYSIS PATIENTS: A SECTIONAL STUDY
Authors: BAHAR Aynur
Published: July 2023

Aim: This study was designed to determine the relationship between depression and spirituality in hemodialysis patients in a dialysis center.

Material and Method: The research is in the type of descriptive research. The current study was conducted with 74 patients hemodialysis. Ethics committee approval and institutional permission were obtained before starting the study. The data of the study was collected by applying “Personal Information Form”, “Beck Depression Inventory” and “Spiritual Well-Being Scale”. The data were collected face to face using the questionnaire method. In the analysis of the data, descriptive statistics, t-test, Anova test and correlation analysis were used.

Results: More than half of the patients were 51 years and over (54.1%) and 60.8% were male. When the Beck Depression Inventory's scores were analyzed according to the cut-off point, it was found that 58.1% of the patients showed depressive symptoms. In the study, BDI mean score was 19.27±7.31, SWBS mean score was 18.74±10.8 (Subscales: Peace 5.70±3.52, Meaning 6.27±3.67, Faith 6.75±3.97). The results of this study revealed that there is a negative significant relationship between mental well-being and depression (p
Comparison of Length of Hospital Stay and Routine Laboratory Parameters in Covid-19 Patients With and Without Serum Vitamin D Deficiency
Authors: UMAY Ayşe , AYDIN Birsen , ÇETİN Şirin , SEZİKLİ Selçuk
Published: July 2023

Amaç :Although some recent studies have shown that serum 25-hydroxyvitamin D (25(OH)D) may be effective on the course of Covid-19 disease, the results obtained are still controversial. Therefore, in this study, it was aimed to examine whether there are differences in terms of age, gender, length of hospital stay, biochemical and hematological parameters between those with and without serum 25(OH)D deficiency in Covid-19 patients.

Gereç ve Yöntem: The data of 413 patients hospitalized in Ankara Pursaklar State Hospital whose covid-19 positivity was revealed by PCR test were evaluated retrospectively. Those with less than serum 25(OH)D (
Empirical assessment of SR/CA small-dollar lending letter impact
Authors: Gorin Daniel , Gosky Sarah , Suher Michael
Published: July 2023

Guidance is used by bank regulators to communicate supervisory expectations to both examiners and banks. In March 2020, in response to pandemic shut-downs, financial regulators issued a joint statement encouraging small-dollar lending to meet temporary cash-flow imbalances, unexpected expenses, or income short-falls.
Islamic Philosophy in Indian-Subcontinent and Middle Asia: An Analysis of Literature Published in The 21st Century
Authors: UDDIN Md Salah
Published: July 2023

Islamic philosophy as a branch of knowledge emerged following the triumph of Greek philosophy. A large number of studies focused on various aspects of Islamic philosophy throughout the world. The present study aims to analyze the articles published in the SCOPUS-indexed journals on different issues of Islamic philosophy in the 21st century from central Asia and the Indian subcontinent. Using bibliometric R-package and Microsoft Excel, the analysis focused on the summary of the data, productivity over the years, analysis of the sources and the areas of study. The number of publications has increased over the years on this topic. However, a significant amount of research has been conducted in Iran and Turkey. Eventually, the contributing sources are also mainly from these two countries. Among the topics covered are related to ‘Islamism’, ‘Islam’, ‘History’, ‘Religion’ and ‘Philosophy’. Future research can be conducted on the contributions of various Islamic philosophers in metaphysics, epistemology, natural philosophy and social philosophy. Based on the current study's findings, future researchers on Islamic philosophy can determine their areas of interest.
İngiltere ve Rusya’nın I. Dünya Savaşı Sırasında ve Sonrasında İran Siyaseti (1914-1925)
Authors: KARADENİZ Yılmaz
Published: July 2023

İran, tarih boyunca üzerinde bulunduğu stratejik konumu ve sahip olduğu yeraltı kaynakları sebebiyle emperyalist devletlerin dikkatini çekmiştir. 19. asrın son yarısından itibaren de İngiltere, Rusya ve Fransa’nın sömürgeci siyasetine maruz kalmıştır. Özellikle 20. asrın başında nüfuzunu İran’da hissettiren İngiltere, aynı topraklarda hedefleri olan Rusya’nın Hindistan güzergâhına sarkmaması ve Fars Körfezi’ne ulaşmaması için bütün diplomatik manevraları devreye sokmuştur. İngiltere, Rusya’nın masa başında oyalanması, İran’ın kuzeydeki topraklarını işgal etmesi ile yetinmesi siyasetinde başarılı olmuştur. Kaçar idaresinin başlarında Fethali Şah döneminde kuzeydeki toprakların işgali ile başlayan İran-Rus Savaşlarının güneye sıçramaması için arabulucu rolü oynamış, bu rolü neticesinde iki devlet arasında imzalanan Gülistan ve Türkmençay antlaşmalarıyla İran’ın ağır savaş tazminatına mahkûm ettirilmesini sağlamıştır. Rusya’ya ödenecek tazminat, mali açıdan İngiliz nüfuzunun kurulmasını kolaylaştırmış, işgal edilen toprakların Rusya’da kalması ise bu devleti durdurmuştur. İran’ı I. Dünya Savaşı öncesinde kendi nüfuz bölgesi olarak gören İngiltere, bir yandan Kaçar idaresini de yıpratarak adım adım hedefine doğru ilerlemiştir. Rusya ise bu hedefin içerisinde rol almak suretiyle İran üzerindeki yayılmacı siyasetini ortaya koymuştur.

Çalışmamızda adı geçen iki sömürgeci ülkenin İran’da gerçekleştirdikleri işgali, İngiltere’nın savaş sırasında temel gıda maddelerini stoklamasıyla ortaya çıkan kıtlık ve toplu ölümlere nasıl sebep olduğunu, Sovyetler Birliği’nin sosyalist ihtilal ile savaştan çekilmesiyle İran’da ortaya çıkan durumu vermeye çalışacağız. Sovyetler Birliği’nin İran siyaseti ve İngiltere’nın diplomatik hamleleri ile bu siyasetin etkisiz hale getirilmesi, Kaçar idaresinde sıkça gerçekleşen hükümet değişiklikleri ekseninde ele alınacaktır. Savaş sonrasında imzalanan İran-İngiltere Anlaşması ile bağımsızlığın elden çıkışı ve Rıza Mirpençe’nin niçin iktidara getirildiğini dönemin kaynakları doğrultusunda ortaya koymaya çalışacağız.
Halikarnas Balıkçısı’nın Düşün Yazılarına Kültür ve Medeniyet Perspektifinden Bakış
Authors: DOĞRAMACIOĞLU Hüseyin
Published: July 2023

Azra Erhat’ın Halikarnas Balıkçısı’nın mektuplarından derlediği Düşün Yazıları adlı eserde kültürel ve tarihsel konularda bilgiler yer almaktadır. Onun bu eseri Halikarnas Balıkçısı’nın edebî eserlerinin fikrî zeminini oluşturmaktadır, denilebilir. Çünkü edebî eserlerinde kullandığı derin tarih bilgisi ve kültür bilinci incelendiğinde yazarın köklü bir bilgi birikimine sahip olduğu ortaya çıkacaktır. Bu bilgilerini roman formatında değil, mektup şeklinde sıralayan yazar, tarih ve edebiyata farklı bakış açıları getirir. Tarih, din, kültür, inanışlar ve diğer konuları farklı bakış açılarıyla ve özgün yorumlarla izah eder. Onun bu yaklaşımı bilgi birikimini edebî hazla birleştirerek aktarma çabasında aranmalıdır. Yazarın mektuplarında konuşma üslûbuyla ve daldan dala atlayarak dağınık bir şekilde vermesi okuyucunun dikkatini canlı tutma isteğine bağlanabilir. Onun bu yazıları incelendiğinde gerek Türk tarihine ve gerekse Akdeniz havzası medeniyetine dair yeni çıkarımlar yaptığı görülebilecektir. Tarih kitaplarında rastlamadığımız ve daha çok mitolojik bilgilerle süslenmiş bilgileri sıralarken kaynak vermekten kaçınan yazar ele aldığı düşünceleri halk kültüründen ve halkın kullandığı sözcüklerden hareketle ispatlama yoluna gider. Onun bu tavrı mektuplarına işlediği konuları özgün ve ilginç bir hâle getirmektedir.
Ankara Numune Hastanesi’nde Alman Bilim İnsanları ve Albert Eckstein
Authors: YILDIZ ÖZLÜ Zeynep , ÜNÜVAR Süleyman
Published: July 2023

Kurtuluş Savaşı tamamlandıktan sonra Mustafa Kemal Atatürk Türkiye’yi çağdaş medeniyetler seviyesine yükseltmek istemiştir. Toplumun ekonomik, kültürel ve sosyal alanda ilerleyebilmesinin ilk şartı ise sağlıklı bireylerin olmasıdır. Cumhuriyet kurulduktan sonra halka sağlık hizmeti vermek için eski Gureba Hastanesi Ankara Numune Hastanesi’ne dönüştürülmüştür. Ancak bu hastane için alanlarında uzman profesörlere ihtiyaç duyulmuştur. Anadolu uzun yıllar savaş halinde olduğu için nitelikli eleman sıkıntısı yaşanmıştır. Hükümet çözüm olarak Almanya’dan profesörleri Türkiye’ye davet etmiştir. Ankara Numune Hastanesi’nde Eduard Melchior, Alfred Marchionini, August Laqueur, Max Meyer, Ernst Magnus- Alsleben ve Albert Eckstein gibi önemli profesörler gelmiştir. Albert Eckstein, Ankara Numune Hastanesi’nde çocuk kliniğini kurmakla görevlendirilmiştir. Türk Pediatrisinin temelini atmıştır. Ülkedeki salgın hastalıkların ve çocuk ölümleri üzerine çalışmıştır. Eckstein, Türkiye’de kaldığı dönemlerde çocuk ölümleri büyük oranda azalmıştır. Anadolu’yu ve köylerini gezmiştir. Süt çocuğu ölümleri ve salgın hastalıklar hakkında incelemelerde bulunmuştur. Bu incelemelerini raporlaştırarak Sağlık Bakanlığına sunmuştur. Köylülere hastalıklarla mücadele yöntemlerini anlatmıştır. Türkiye’de önemli çocuk doktorlarının yetişmesini sağlamıştır. 1949 yılında Türkiye’den ayrılıp, Almanya geri dönmüş ve Hamburg Tıp Fakültesi Çocuk Kliniğinde göreve başlamıştır. Bu makale Ankara Numune Hastanesi’ne gelen Alman Bilim Adamlarının hem hastaneye hem de ülkenin sağlık politikasına katkıları anlatılmak istenmiştir. Özellikle çocuk doktoru Albert Eckstein’in Türkiye’deki çalışmaları, pediatri alanındaki başarıları dile getirilmiştir. Alman bilim insanlarının çalışmaları sayesinde önemli Türk doktorları yetişmiştir. Türk tıbbı çalışmalarından dolayı her zaman minnettar olmuştur
Eski Türk Yazıtlarında Savaş, Mücadele, Ordu, Kahramanlık Gibi Unsurlara Dair Eş Anlamlı İsimler
Authors: ŞAHİN Ayşegül
Published: July 2023

Anlam biliminin inceleme alanlarından olan eş anlamlılık, dildeki zenginliğin en önemli göstergelerinden biridir. Ancak dilde yüzde yüz bir eş anlamlılığın olup olmadığı hususunda pek çok görüş ortaya konulmuştur. Çalışmamızda bu görüşlere kısaca değinilmiş; Eski Türk Yazıtlarında yer alan savaş, mücadele, ordu ve kahramanlığa dair eş anlamlı sözler (isimler) tespit edilerek örneklerle incelenmiş ve Türkçenin tarihi dönemlerinde kullanımları tanıklanmaya çalışılmıştır.
Synthesis, structure and electromagnetic properties of FeCoCu/C nanocomposites
Authors: Muratov D. G. , Kozhitov L. V. , Zaporotskova I. V. , Popkova A. V. , Tarala V. A. , Korovin E. Yu. , Zorin A. V.
Published: July 2023

FeCoCu ternary nanoparticles distributed and stabilized in the carbon matrix of FeCoCu/C metal-carbon nanocomposites have been synthesized using controlled IR pyrolysis of precursors consisting of the “polymer / iron acetylacetate / cobalt and copper acetates” type system obtained by joint dissolution of components followed by solvent removal. The effect of the synthesis temperature on the structure, composition and electromagnetic properties of the nanocomposites has been studied. By XRD was shown that the formation of the FeCoCu ternary nanoparticles occurs due to the interaction of Fe3С with the nanoparticles of the CoCu solid solution. An increase in the synthesis temperature leads to an increase in the size of the metal nanoparticles due to their agglomeration and coalescence as a result of matrix reconstruction. Furthermore, ternary alloy nanoparticles having a variable composition may form depending on the synthesis temperature and the content ratio of the metals. Raman spectroscopy has shown that the crystallinity of the carbon matrix of the nanocomposites increases with the synthesis temperature. The frequency responses of the relative permittivity and permeability of the nanocomposites have been studied at 3–13 GHz. It has been shown that a change in the content ratio of the metals noticeably increases both the dielectric and the magnetic losses. The former loss is caused by the formation of a complex nanostructure of the nanocomposite carbon matrix while the latter one originates from an increase in the size of the nanoparticles and a shift of the natural ferromagnetic resonance frequency to the low-frequency region. The reflection loss has been calculated using a standard method from the experimental data on the frequency responses of the relative permittivity and permeability. It has been shown that the frequency range and the absorption of electromagnetic waves (from –20 to –52 dB) can be controlled by varying the content ratio of the metals in the precursor. The nanocomposites obtained as a result of the experiment deliver better results in comparison with FeCo/C nanocomposites synthesized under similar conditions.
Application of Al2O3 film for stabilization of charge properties of the SiO2/p-Si interface
Authors: Kim A. S. , Serko N. A. , Khakuashev P. E. , Kolky A. N. , Yurchuk S. Yu.
Published: July 2023

The influence of aluminum oxide films obtained by high-frequency cathode sputtering of an Al2O3 target in argon atmosphere on charging properties of the SiO2/p-Si interface was investigated. High-frequency C-V characteristics for MIS-structure with one-layer dielectric films: SiO2 (0,10 µm and 0,36 µm), Al2O3 (0,14 µm) – and its double-layers compositions were measured. Experiment was carried out with a KDB-4.5 and a KDB-5000 substrates. Some electrophysical parameters of the obtained films such as UFB and Qss were calculated. Based on experimental results it was confirmed that the embedded negative charge of Al2O3 film prevented the formation of the inversive layer on p-Si surface by compensation of the embedded positive charge of SiO2 film and enhancement of semiconductor surface with majority charge carriers and, thus, allowed stabilization of charge properties of the SiO2/p-Si interface. The applicability of Al2O3 film as additional dielectric covering for manufacture technology of photodiodes on high-resistance p-Si was confirmed by applying on a multi-element p-i-n photosensitive element (PE) as an example. It was established that passivation of silicon dioxide on periphery and between the elements of PE by Al2O3 film improved I-V characteristics and insulation resistance, which lead to increased yield rate of photodiodes.
Selectivity of the bioherbicidal potential of Artemisia arborescens L. among model and agronomic crops and implications for pre- and post-emergence applications
Authors: Ilehi Oumayma , Chograni Hnia , Ben Rejeb Fedoua , Riahi Leila
Published: July 2023

Background: The efficacy and selectivity of phytochemicals are required for the development of new botanical bioherbicides for both pre- and post-emergence applications.

Questions: How vary the phytotoxic potential of the aqueous extracts of Artemisia arborescens among five plant species?

Studied species: Lactuca sativa used as a model species, two monocotyledons (Triticum turgidum, Zea mays) and two dicotyledons (Raphanus sativus, Pisum sativum).

Study site and dates: Two Artemisia arborescens genotypes were collected at Bousselem and Kef, North West of Tunisia; March 2021.

Results: The aqueous extracts are rich in polyphenols, flavonoids, condensed tannins and terpenoids. The highest in vitroinhibitory potential on germination and seedlings growth was detected against Lactuca sativa. The concentration 8 mg/mL causes total inhibition of lettuce germination and has no significant effect on pea germination. At this concentration, the extracts inhibited the germination of the agronomic crops by 46-58 % (Raphanus sativus), 74 % (Zea mays) and 72-78 % (Triticum turgidum). The inhibition rates of radicles and shoots growth vary according to the applied concentration. The in vivo tests showed higher phytotoxic activity against Lactuca sativa and both monocotyledons. This was confirmed by the variation of the symptomatic and biochemical traits.

Conclusions: The obtained results confirm the selective bioherbicidal activity of A. arborescens water extracts which promote its potential as natural resource for the development of new botanical bioherbicides.
Iohannes Serifaber Zrínyi Miklósnak címzett két verse 1550-ből
Authors: Molnár Dávid
Published: July 2023

A fiatalon elhunyt Iohannes Serifaber két, idáig ismeretlen verse 1550-ből az akkor még viszonylag ismeretlen horvát-szlavón bánról, Zrínyi Miklósról. Az egyik vers egy korai, szintén ismeretlen Zrínyi-portréhoz íródott.
Hozzászólás Bezeczky Gábor Szinnyei József és a hazai hírlapirodalom című írásához
Authors: Gazda István
Published: July 2023

A Magyar Könyvszemle 2021-es évfolyama 3. számában megjelent cikk helyesbítése.
Amália Kerekes, Wartezeit, Studien zur Geschichte der ungarischen Emigration in Wien, 1919–1926, Würzburg, Königshausen & Neumann, 2018
Authors: Rózsa Mária
Published: July 2023

Kerekes Amália, az ELTE Német Irodalom és Kultúra Tanszéke habilitált egyetemi adjunktusának könyve a magyar szociáldemokrata és kommunista emigráció képviselőinek bécsi publicisztikai tevékenységét, politikai nézeteinek alakulását követi végig az 1920-as évek közepéig.A bécsi emigráció eddigi historiográfiai és irodalomtörténeti feldolgozásának eredményeit ismertetve a szerző a sajtóbeli, levéltári, illetve a hézagosan fennmaradt rendőrségi dokumentumok segítségével mutatja be az emigránsok befogadásának állomásait.
Nők, időszaki kiadványok és nyomtatott nyilvánosság, 1820–1920, szerk. Török Zsuzsa, Budapest, Reciti, 2020
Authors: Rózsa Mária
Published: July 2023

A kötet anyagát a Bölcsészettudományi Központ által 2019. október 14–15-én megrendezett „Nőszerzők a 19. században: lehetőségek és korlátok” című konferencián elhangzott előadások szerkesztett változata adja. A tematikus vezérelv a női szerzőktől származó írások és a periodikusan megjelenő kiadványok kapcsolata kronologikusan áttekintve az egy évszázadot átfogó időszakot.A publikáló szerzők (nemüktől most eltekintve) ismertté válását nagymértékben elősegítette a 19. században az időszaki kiadványokban történő megjelenés, gyakran ezt követte műveiknek kötetekben való közlése. Ugyanakkor a vizsgált időszakban gyakran találunk példát a fordítottjára is, a megjelent kötetek anyagát egyes periodikumok másodközlés formájában népszerűsítették.
EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO) v1
Authors: Holzer Elisabeth
Published: July 2023

This protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP. The expression is performed with E. coli Rosetta pLysS cells. The protein is purified via a GST batch purification and gel filtration (SEC).
Fixing hippo neurons to assess endogenous NEMO during oxidative stress v1
Authors: HARDING OLIVIA
Published: July 2023

We altered a previously developed method (dx.doi.org/10.17504/protocols.io.bxpfpmjn) in order to investigate the interaction of endogenous NEMO with damaged mitochondria in Hippocampal rat neurons. Mitochondrial damage was induced by the administration of 35 nM Antimycin A, a complex III inhibitor, over 1 hr. Here, we visualized neuronal mitochondria with a mito-targeted construct, Mito-SNAP, and a fluorescent SNAP ligand, JaneliaFluor 646, though other mitochondrial markers may be suitable. Endogenous NEMO was visualized with the commercially available anti-NEMO primary antibody (abcam). AntA-treated neurons exhibited fragmented mitochondria. We did not observe a change in the appearance of NEMO labeling in AntA treated cells versus non-treated cells.
TCR sequencing and activation v1
Authors: Jonathan Zorea
Published: July 2023

This protocol is a procedure we follow to isolate tumor infiltrating T cells and try to activate them with unique peptides and to characterize their TCR repertoire
Fixation and imaging of HeLa cells after mitochondrial depolarization v1
Authors: HARDING OLIVIA
Published: July 2023

Ectopic expression of p62/SQSTM1 often induces puncta formation and poor cell health due to p62’s proclivity to multimerize and form filaments. We used the cell fixation protocol described here in order to over-express fluorescently tagged p62 at low levels in p62-/- cells and amplify the signal with immuno-labeling. By this method, we were able to express a variety of tagged p62 mutations to determine their effects on NEMO recruitment to depolarized mitochondria without introducing toxic artifacts of p62 overexpression. We also note the use of a modified fixation protocol to visualize various endogenous ATG8 proteins, which are also difficult to over-express in our system. Thus, fixation techniques are a critical complement to studies in live cells.
Live imaging to investigate mitophagy kinetics and NEMO recruitment in HeLa-M cells v1
Authors: HARDING OLIVIA
Published: July 2023

There is no substitute for live cell imaging in the investigation of the kinetics of subcellular biology. Here, we enumerate a protocol to visualize fluorescently-tagged mitochondria, NEMO, OPTN, and p62 during the cellular response to mitochondrial depolarization. Because live cells are sensitive to photo-damage, we used low laser power and short exposure times in our confocal microscopy system. Even with minimally damaging parameters, we were able to collect high-content data that we subsequently analyzed. With this technique, we showed that NEMO and OPTN, despite containing highly similar domains, were recruited to damaged mitochondria with less correlation than NEMO and p62. Furthermore, live imaging of NEMO occupancy on damaged mitochondria was a necessary complement to our parallel fixation studies. Since fixation introduces artifacts, especially in samples with concentrated proteins like those in mitophagy, results from our live cell imaging corroborated our findings in fixation studies. Our reporting of these results would not have been possible without real-time, live cell imaging.
HeLa culture, transfection, and labeling of Halo-fusion proteins v1
Authors: HARDING OLIVIA
Published: July 2023

High-throughput, predictable systems that are easily modulated are ideal for the study of cell biology. Here we developed a protocol to investigate the role of the Nuclear Factor kappa-B Effector Molecule (NEMO) in Parkin-dependent mitochondrial clearance. Transient transfection of fluorescent constructs allowed us to visualize subcellular structures and dynamics while maintaining flexibility in a consistent model system. The EGFP-NEMO plasmid was repeatedly employed to study NEMO interactions during mitophagy, and we were also able to edit the construct to create both a NEMO point mutation and a Halo-tagged NEMO construct, which we readily expressed in HeLa cells. Halo-fusion constructs, including NEMO and OPTN used in our study, allowed us to visualize the exogenously expressed proteins conjugated to chemical ligands in a variety of colors. This and the accompanying protocols were critical to our characterization of NEMO’s involvement in mitophagy.
Pre-service Teachers’ Comprehension of Renewable Energy Sources
Authors: ŞEN Şenol , TEMEL Senar
Published: July 2023

This study aims to identify pre-service science teachers’ comprehension of renewable energy sources. The study was conducted with a qualitative research method. 33 pre-service science teachers were included in the study group. A purposeful sampling method was used in choosing the participants in the study. The study data were collected through 15-20-minute semi-structured interviews. The interview form containing questions prepared by the researchers on why to prefer renewable energy sources, whether or not the use of renewable energy sources was necessary, and how to promote the use of those sources was used in the interviews. All the interviews were voice recorded by taking the consent of the participants. The data obtained from the interviews were transcribed and then put into a computer by the other researcher and analyzed by content analysis. During the analysis, themes were created after the codes and categories were determined. After that, the data were divided into themes. The findings were considered in three themes and the results obtained were evaluated generally. Although it was considered a result that the pre-service science teachers stated their views in three different themes, the fact that the low rates of the views stated can be interpreted as that their comprehension of renewable energy sources is inadequate.
Potential Impact of Waterway Development on Cultural Landscape Values: The Case of the Lower Vistula
Authors: Golędzinowska Anna
Published: July 2023

The northern (“lower”) section of the Vistula is on the route of two international waterways—E70 and E40. However, the current condition of the riverbed prevents larger vessels from passing through. Plans for the waterway date back to the beginning of the 20th century. Following Poland’s ratification of the European Agreement on Main Inland Waterways of International Importance in 2017, the general concept has been transformed into more concrete studies and has found its place in the national development policy. The scientific and political discourse primarily addresses the potential benefits of river regulation in the field of transport and energy. Against this background, studies on the impact of investments on the natural environment are published less frequently. Meanwhile, the Vistula has for centuries influenced the formation of a unique cultural landscape, which will be severely transformed if the river is regulated. On the other hand, insufficient transit depths of the waterway result in the loss of the function of the historic transport corridor, which also changes the character of parts of the area dependent on the river—in particular, the riverside areas of towns. This article aims to indicate the need for a qualitative landscape assessment of how the impact of investments is assessed and the best solution chosen. Using the assumptions of the historic urban landscape, the author analyses the potential impact of the planned investment in the lower Vistula on the surrounding cultural landscape. The potential scope for change in two dimensions is indicated at the scale of the lower Vistula and the individual towns. The possible impact of the investments on the panoramas is illustrated for selected cases.
Literature Study: Visual Support Design as Assistive Technology for Children with Autism Spectrum Disorder
Authors: Irvan Muchamad , Jauhari Muhammad Nurrohman , Wasesa Andarmadi Jati Abdhi , Rosmi Yandika Fefrian
Published: July 2023

Providing interventions for children with autism requires various methods and learning media. Visual Support has long been implemented to help train communication skills in children with autism. Recently, many applications have developed as assistive technology to help children with autism in the learning environment. This study aims to elaborate on the visual design integrated into application-based assistive technology for children with autism. The method used in this research is a Systematic Literature Review. Based on the data tracing results, several references have been collected that specifically discuss the design of visual Support. From the data analysis, it can be concluded that visual Support is important to be integrated into digital applications to help children with ASD to carry out daily activities in various environmental settings. The development of visual support design must refer to the prerequisite rules to support the suitability of their characteristics and needs.
PENGEMBANGAN E-MODUL INTERAKTIF HEYZINE FLIPBOOK BERBASIS SCIENTIFIC MATERI SIKLUS AIR BAGI SISWA KELAS V SEKOLAH DASAR
Authors: Manzil Emilda Farkhiatul , Sukamti Sukamti , Thohir M. Anas
Published: July 2023

The purpose of this research is to develop an interactive scientific-based electronic module that is valid according to material experts and media experts and is suitable for users (teachers) and students. The model used is the ADDIE model which includes five stages, namely (1) Analyze, (2) Design, (3) Development, (4) Implementation, and (5) Evaluation. This study uses observation, interviews, and questionnaires as data collection instruments. The material validity test score was 93 percent, the media validity test score was 75 percent, the teacher feasibility test score was 98 percent, and the student test score was 96.5 percent and received a positive response from the students. From the results of these scores, the electronic module for class V water cycle materials in elementary schools is classified as very valid and feasible to use. Tujuan dari penelitian ini yaitu mengembangkan modul elektronik interaktif berbasis scientific yang valid menurut ahli materi dan ahli media, serta layak bagi pendidik (guru) dan peserta didik. Model yang digunakan adalah model ADDIE yang mencakup lima tahap yakni (1) analisis, (2) desain, (3) pengembangan, (4) implementasi, dan (5) evaluasi. Penelitian ini menggunakan observasi, wawancara, dan angket sebagai instrumen pengumpulan data. Skor uji validitas materi diperoleh sebesar 93 persen, skor uji validitas media sebesar 75 persen, skor uji kelayakan guru sebesar 98 persen, dan skor uji coba peserta didik sebesar 96,5 persen serta mendapat respon positif dari peserta didik. Dari hasil skor tersebut, modul elektronik untuk materi siklus air kelas V di Sekolah Dasar tergolong sangat valid dan layak digunakan.
Pengembangan Media Pembelajaran E-Materi untuk Meningkatkan Minat dan Motivasi Belajar Mahasiswa pada Matakuliah Konsep Dasar PPKn
Authors: Mulyani Mulyani , Prabowo Suryo Hadi Wira , Wicaksono Vicky Dwi , Paksi Hendrik Pandu
Published: July 2023

E-Material through multimedia can make the learning process more interesting so that it is easier to understand. Good multimedia is able to present various kinds of events that can be used as learning media. E-Material is intended to motivate students to learn by attracting their interest and piquing their curiosity. Thus, E- Material is thought to be able to increase competence in the Basic Concepts of Civics. The purpose of this study was to find out the results of developing E-Material products as learning media in the Civics Basic Concepts course which was declared feasible by validation experts and student limited trials. ASSURE is the development methodology utilized for this study. Based on the score obtained from the expert validation test and limited trial, it was stated that the E-Material made was declared valid in terms of the material contained and the use of learning media. These results can be concluded that the compiled E-Material can be used on elementary school teacher education students. Multimedia E-Materi dapat membuat proses pembelajaran lebih menarik dan karenanya lebih mudah untuk dipahami. Multimedia yang baik mampu menyajikan berbagai agenda yang dapat digunakan sebagai media pendidikan. Diharapkan E-Materi akan menarik perhatian dan minat mahasiswa, sehingga memotivasi mahasiswa untuk belajar. Dengan demikian, E-Materi diyakini dapat meningkatkan pengetahuan tentang Konsep Dasar PPKn. Penelitian ini berusaha untuk mengetahui hasil pengembangan produk E-Materi sebagai media pembelajaran pada mata kuliah Konsep Dasar PPKn yang dinyatakan layak oleh ahli validasi dan uji coba terbatas mahasiswa. ASSURE adalah metodologi pengembangan yang digunakan untuk penelitian ini. Berdasarkan skor yang diperoleh dari uji validasi ahli dan uji coba terbatas menyatakan bahwa E-Materi yang disusun dinyatakan valid secara materi yang terkandung dan penggunaan media pembelajaran. Hasil tersebut dapat disimpulkan bahwa E-Materi yang disusun dapat digunakan pada mahasiswa Pendidikan Guru Sekolah Dasar.
Analisis Penerapan Pembelajaran Berdiferensiasi Pendidikan Pancasila dan Kewarganegaraan (PPKn) dalam Kurikulum Merdeka Sekolah Dasar
Authors: Ramadhan Wandri , Rifana Fitria , Meisya Rovika , Putro Khamim Zarkasih , Frasandy Rendy Nugraha
Published: July 2023

This study aims to reveal the process of implementing differentiated learning and the implementation factors of Civics learning using differentiated learning in 4th grade. This qualitative research uses data in the form of descriptive data such as writing, speech, and behavior that can be observed. The research was conducted in the even semester of 2022 at SDN Adisucipto 1 Yogyakarta. Teacher research informants and grade IV students. Differentiated learning in Civics subjects carried out in class IV of SDN Adisucipto 1 Yogyakarta has a good impact on teachers and students in learning which is described in its application which consists of three stages: content differentiation, process, and product. Furthermore, the factors of differentiated learning implementation in PPKn class IV were also obtained, namely effective learning strategies and active student involvement.Penelitian ini bertujuan untuk mengungkapkan proses pelaksanaan pembelajaran berdiferensiasi dan faktor keterlaksanaan pembelajaran PPKn menggunakan pembelajaran berdiferensiasi di kelas IV. Penelitian kualitatif ini menggunakan data berupa data deskriptif seperti tulisan, perkataan dan perilaku yang bisa diamati. Penelitian dilakukan pada semester genap 2022 di SDN Adisucipto 1 Yogyakarta. Informan penelitian guru dan siswa kelas IV. Pembelajaran berdiferensiasi dalam mata pelajaran PPKn yang dilaksanakan di kelas IV SDN Adisucipto 1 Yogyakarta memberikan dampak yang baik bagi guru dan siswa dalam pembelajaran yang diuraikan dalam penerapannya yang terdiri dari tiga tahap: diferensiasi konten, proses dan produk. Selanjutnya, juga diperoleh faktor keterlaksanaan pembelajaran berdiferensiasi dalam PPKn kelas IV, yaitu strategi pembelajaran yang efektif dan keterlibatan siswa yang aktif.
Pengembangan Aplikasi Berbasis Android untuk Media Membaca Permulaan Siswa Kelas I Sekolah Dasar
Authors: Erlina Tiara , Iswara Prana Dwija
Published: July 2023

This study aims to produce an Android-based application for reading media in grade I elementary schools such as difficulty remembering the shapes and sounds of letters, distinguishing similar letter shapes, the pronunciation of letters that are not fluent, and removing letter sounds and words. The research subjects used in this research were grade I elementary school students in a Sumedang Regency. The RnD procedure with the ADDIE model produces a description of the feasibility of the application obtained from the assessment of material experts and design experts. The data collection process and data processing used interview instruments, questionnaires, and scoring guidelines. Based on the results of the assessment, the material validation value reached 90.63 percent and the design validation value reached 75 percent. So, this Android-based application meets the eligibility criteria, so it can be used in early reading learning in class or as an independent learning media.Penelitian ini bertujuan untuk menghasilkan aplikasi berbasis Android untuk media membaca di kelas I sekolah dasar seperti kesulitan mengingat bentuk dan bunyi huruf, membedakan bentuk huruf yang mirip, pelafalan huruf yang tidak fasih, serta terjadi penghilangan bunyi huruf dan kata. Subjek penelitian yang digunakan dalam penelitian ini adalah siswa kelas I sekolah dasar di sebuah Kabupaten Sumedang. Prosedur RnD dengan model ADDIE ini menghasilkan deskripsi kelayakan aplikasi yang diperoleh dari penilaian ahli materi dan ahli desain. Proses pengambilan data dan pengolahan data menggunakan instrumen wawancara, kuesioner, dan pedoman penskoran. Berdasarkan hasil penilaian, nilai validasi materi mencapai 90,63 persen dan nilai validasi desain mencapai 75 persen. Dengan demikian, aplikasi berbasis Android ini memenuhi kriteria kelayakan, sehingga dapat digunakan dalam pembelajaran membaca permulaan di kelas atau sebagai media belajar mandiri.
The Career Development Model of Students of Private Vocational High School Veteran 1 of Tulungagung during the Pandemic
Authors: Pratiwi Niken Hari , Nurhadi Didik , Yoto Yoto
Published: July 2023

The online learning system during the pandemic of COVID-19 has changed the system for conselling services in such a way that guidance and counselling teachers at vocational high schools are required to be creative and innovative in performing services for students online. They are shouldering more responsibilities because during the pandemic the rate of employment termination has increased, while that of job vacancies has decreased, which constitutes a serious challenge for students’ career development. They are also faced with the problem of exploring career choices, finding good jobs in accordance with students’ interests and characters, and improving students’ career prospects withouth meeting those students in person. This research focused on knowing the career development model of the students of Private Vocational High School (PVHS) Veteran 1 of Tulungagung during the pandemic. This was qualititative approach with descriptive research in which the data obtained from interviews and observation were presented in a descriptive report which can be easily understood. The research findings shows that guidance and counselling teachers are able to follow technological advances, foster innovation, and establish good communication with industries and business world during the pandemic of COVID-19. Guidance and counselling teachers of PVHS Veteran 1 of Tulungangung continues to provide counselling services for career development by combining online and offline models in a scheduled appoinment by still obeying health protocols.
The Human Capital of Technicians in State University of Malang
Authors: Pratama Aditya Yudha , Nurhadi Didik
Published: July 2023

Agencies or companies' ability to create and develop strategies to advance depend on human capital. It receives public attention as tangible state assets, policies, and organizations and are described as the core vital forces in the professional world. It is essential because it provides companies with some new skills, opportunities, and even development. This study aims to describe the human capital (knowledge, competence, skill, and innovation) of the technicians in the State University of Malang using a survey research design. The population is the technicians in the State University of Malang. The result indicates that the human capital in the State University of Malang can be categorized as follows: 3.1 percent of the technicians are very positive (one technician), 31.3 percent are (ten technicians), 34.4 percent are average (11 technicians), 28.1 percent are negative (nine technicians), and 3.1 percent are very negative (one technician). It can be concluded that the human capital of the State University of Malang is average, which implies that not all the employees can perform well at work and meet the indicators; hence, the need for improvement.
A Bibliometric Analysis of the Employability Skills Research Trends in Vocational Education
Authors: Maharbid Allan Anugraha , Kustiawan Iwan
Published: July 2023

Employability skills are the skills needed to acquire, retain, and do a good job. Employability skills are essential in helping prepare students to increase competitiveness and work productivity. The world of work wants graduates to be competitive and technically competent with skills relevant to technological developments. This study aims to analyze research trends related to employability skills in vocational education. We use bibliometric analysis procedures on 1123 published articles in the Scopus database. The results of this study reveal citation, co-authorship, co-citation, and keyword analyses producing a pattern of research maps in vocational education. The findings identify publication trends, widely cited articles, contributing countries, productive journals, and obtain information for future research on employability skills
The 4C Learning Innovation as an Effort to Improve Teacher's Professionalism and Students' Competency at Private Vocational High School of Walisongo 2 Gempol, Indonesia
Authors: Afifudin Afifudin
Published: July 2023

This research was conducted to observe the efforts of a private school to improve teachers’ professionalism and students’ competency. This study used a case study model with a descriptive qualitative approach. Here, data collection was done through in-depth interviews, observation, and document study. The results showed that teachers with knowledge and motivation improve their service to students through teaching skills. Teachers’ knowledge was a learning innovation to the better learning process. They had the 4C skills: critical and creative thinking, collaboration, communication, and creativity. Teachers’ professionality then affected students’ competency, be it soft and hard skills. However, the evidence that was found at Private Vocational High School of Walisongo 2 Gempol, Indonesia could not be generalized to all private vocational high schools. Thus, further research on other private schools in Indonesia is needed to develop creativity and innovation to support graduates’ quality
Analisa Karakter Yang Baik dalam Sistem Pendidikan di Era Masyarakat 5.0
Authors: Putri Syarifa Dhea Fanny
Published: July 2023

The education sector is currently experiencing a rapid revolution that presents various challenges in adapting to the changing times. This revolution has both positive and negative impacts on education and character. The aim of this research is to address the challenges and strategies for character education in the era of society 5.0. The implementation of character education in the education system should focus on good character aspects, which are categorized into three: moral knowledge, moral feelings, and moral actions. The study used qualitative descriptive analysis, which revealed that character education in the era of society 5.0 is a deliberate effort to instill morals and ethics in personal, social, and daily life. Good character values have become crucial in this era of disruption, and there are two models of good character disruption in the education system: substantive and reflective.

Dunia pendidikan saat ini sedang menghadapi  revolusi yang sangat cepat dengan berbagai tantangan  sehingga  dapat dengan mudah  beradaptasi sesuai  dengan  kondisi  dan  tuntutan  zaman. Dengan adanya revolusi ini banyak dampak positif dan negatif yang berpengaruh pada Pendidikan begitu juga karakter. Penelitian ini di latar belakangi oleh tantangan dan strategi pendidikan karakter dalam menyongsong masa masyarakat 5.0. Implementasi pendidikan karakter dalam sistem pendidikan harus mempertimbangkan aspek - aspek karakter yang baik. Aspek - aspek karakter yang baik umumnya diklasifikasikan menjadi tiga (3) aspek yaitu pengetahuan tentang budi pekerti (pengetahuan moral), kebiasaan karakter (perasaan moral), dan tindakan yang digerakkan oleh karakter (tindakan moral). Cara yang dipakai pada analisa kali ini menggunakan analisis secara kualitatif deskriptif. Reaksi dari analisa yang telah dikemukakan menghasilkan sesuatu hal penting yaitu pendidikan karakter dalam sistem pendidikan di era society 5.0 merupakan tindakan yang sadar dan terencana yang dilakukan manusia dengan memiliki moral dan etika dalam kehidupan pribadi, sosial, dan kebangsaan kehidupan. Nilai-nilai karakter yang baik sudah menjadi prioritas utama untuk dikembangkan pada masa masyarakat 5.0, mengacu pada nilai-nilai karakter yang baik pada era disrupsi. Secara umum, ada dua (2) model disrupsi karakter yang baik pada sistem pendidikan di era masyarakat 5.0, yaitu substantif dan reflektif.