bluuebunny/crossref_metadata_2025 huggingface dataset:
The Design and Optimization of Natural Gas Liquefaction Processes: A Review
Authors: Gao Lei , Wang Jiaxin , Binama Maxime , Li Qian , Cai Weihua
Published: October 2022

As the energy crisis intensifies, the global demand for natural gas is growing rapidly. Liquefied natural gas (LNG) technology is among the delivery solutions with flexible and reliable application prospects and is already a significant field of research in energy utilization. The performance of natural gas liquefaction process has a major influence on the production capacity, energy consumption, economics, and safety of the entire supply chain. Many scholars have conducted numerous studies on various LNG processes and designed many classical processes. This paper summarizes and discusses current research status and development level in the design and optimization of natural gas liquefaction processes in recent years, mainly focusing on cascade liquefaction process, expansion liquefaction process, and mixed refrigerant liquefaction process. The advantages and disadvantages of various liquefaction processes are compared and analyzed in terms of liquefaction capacity, energy consumption, economy, safety, and adaptability. In addition, the rapid development of pressurized liquefaction technology in recent years and its application outlooks are also introduced in detail. Finally, the present situation and industrial demand of LNG process are analyzed, and reasonable suggestions and future research prospects are put forward.
Remote Sensing-Based Estimation on Hydrological Response to Land Use and Cover Change
Authors: Ding Ying , Feng Huihui , Zou Bin
Published: October 2022

Hydrological processes are an important driving force of environmental pollutant variation that has aroused global concern. Land use and cover change (LUCC) strongly affects hydrological processes. Remote sensing technology has played an increasingly important role in studying the relationship between LUCC and hydrological processes. This study summarizes the progress of hydrological responses to LUCC. Overall, remote sensing can provide spatially continuous data of land cover and hydrological variables. With the aid of the retrieved data sets, the effects of LUCC on hydrological processes can be evaluated via correlation analysis, multiple regression method, experimental watershed approach and trajectory-based approaches. However, due to the high complexity of geographical systems, it is difficult to quantitatively separate the actual components of the influence of LUCC. The heterogeneous surface properties also lead to various results at different spatial and temporal scales. Future research should meet the challenges in data estimation, research methodology and analysis process.
The Impact of Collective Forestland Tenure Reform on Rural Households’ Inputs: Moderating Effects Based on Off-Farm Employment
Authors: Xiao Hui , Xie Yang , Hou Fangmiao , Li Xiaoyi
Published: October 2022

Collective Forestland Tenure Reform has confirmed the forestland tenure of rural households and made forestland property rights clearer. In order to explain whether this policy is effective in improving rural households’ expected returns and sense of forestland tenure security, we built models to study the impact of off-farm employment on forestland input in the context of labor migration to urban areas. We used data from the rural household tracking survey conducted by the Development Research Center of the National Forestry and Grassland Bureau from 2003–2016, which includes nine provinces (districts) and 1227 sample rural households in China. Regression models with the forestland titling program as the key influencing factor were constructed, controlling for household characteristics, household head characteristics, forestland characteristics, village level characteristics, market characteristics, and policy factors. Forestland leases had no significant on cash outlays and labor inputs. Forest tenure mortgage loans had a significant positive effect on cash outlays and labor inputs. For households’ off-farm employment, the moderating effects of labor migration on labor inputs and cash outlays are modeled separately. The study indicated that the forestland tenure titling certificates increase households’ enthusiasm in forestland production and promote cash outlays and labor inputs in forestland management. The results regarding the moderating effect indicated that labor migration has a positive moderating effect on rural households’ forestland inputs including labor inputs and cash outlays.
Residents’ Preferences to Multiple Sound Sources in Urban Park: Integrating Soundscape Measurements and Semantic Differences
Authors: Gong Yuan , Cui Chuyun , Cai Mengmeng , Dong Zehao , Zhao Zheng , Wang Aihua
Published: October 2022

Urban parks provide their residents with a space for activities and interactions, and soundscapes play an important role in influencing the residents’ sense of experience of the park environment, with the result that the preferences of residents regarding multiple sound sources can be used as the basis of improving the soundscape quality and optimizing the function of urban parks. Using Shanghai Century Park as the research area, this study focused on the residents’ preference to multiple sound sources based on registered residence differences, and comprehensively used the semantic differential method, importance-performance analysis model, and multinomial logit model to conduct quantitative research on urban park soundscapes and combined the measurement results of the soundscape with the residents’ perception and preference. The results showed that the artificial sounds in Century Park were noisy during the morning and evening peak, while the human sounds were noisy when residents gathered in the park. There was a close relationship between the favorability and subjective loudness of urban park soundscapes, and the residents’ perceptions of urban park soundscapes substantially differed in terms of loudness, tone, and sense of belonging, whereas the differences in the perception of timbre, coverage, and sound source distance were relatively small. Furthermore, it is necessary to enhance the role of natural sounds as well as control the main noise-producing sound sources so that various sounds in the park will not interfere with each other; the optimization of soundscapes should also focus on the residents’ different soundscape preferences due to their individual characteristics.
Agroforestry Systems in Wine Production-Mitigating Climate Change in the Mosel Region
Authors: Riekötter Nicklas , Hassler Markus
Published: October 2022

As Climate Change starts to show substantial impact on viticulture, winemakers are under pressure to implement sustainability transformation to maintain future production. This article deals with the effects of agroforestry system use on wine production and marketing in the German Mosel Region and reviews its challenges and benefits regarding sustainability transformation. The study is based upon qualitative interviews and field visits, researching farmers’ experiences with an agroforestry vineyard in the cool climate wine growing region and reviewing the production methods on-site. Applying Sustainability Levers Theory, the article shows that the use of agroforestry in viticulture targets deep leverage points of the food production system, enabling sustainability transformation by providing positive effects on production and marketing. The article thus concludes that there’s a potential of mitigating challenges of climate change, sustaining the wine production due to the implementation of agroforestry.
Micro-Aerobic Pre-Treatment vs. Thermal Pre-Treatment of Waste Activated Sludge for Its Subsequent Anaerobic Digestion in Semi-Continuous Digesters: A Comparative Study
Authors: Castillo Alejandra , Ortega-Martínez Eduardo , Pagés-Díaz Jhosané , Montalvo Silvio , Huiliñir Cesar
Published: October 2022

This article investigates methane production, organic matter removal, and energy by comparing micro-aerobic pre-treatment and thermal pre-treatment of waste-activated sludge (WAS). For micro-aerobic pre-treatment, WAS was pre-treated at 0.35 vvm (volume of air per volume of medium per minute) for 48 h. The data showed over a 30% increase in soluble Chemical Oxygen Demand (COD) and soluble proteins when this pre-treatment was applied. Then, the micro-aerobically pre-treated sludge was mixed with primary sludge and anaerobically digested in semi-continuous digesters with Hydraulic Retention Times (HRT) of 20, 15, and 10 days at 35 °C. We used two digesters as a control: one fed with a mixture of primary sludge (PS) and raw WAS; another fed with a mixture of PS and thermally pre-treated WAS. The results showed a better performance for the digester fed with micro-aerobically pre-treated sludge than the other two at all the HRT tested. The better performance is because of the solubilization of particulate organic matter, as shown at the reactor outlet. Energy consumption analysis showed that micro-aerobic pre-treatment required 32% more energy in a year than thermal pre-treatment. However, if sludge is pre-thickened in a similar way as performed for thermal pre-treatment, then the energy demand required by micro-aerobic pre-treatment is reduced by 41% concerning the thermal pre-treatment; nevertheless, more studies should be performed to verify that methane production and solid reduction advantages are maintained.
Food Waste Treatments and the Impact of Composting on Carbon Footprint in Canada
Authors: Thapa Pradeep , Hasnine MD Tanvir , Zoungrana Ali , Thakur Sandeep , Yuan Qiuyan
Published: October 2022

Forty percent of the food generated in Canada is wasted, making it the most significant component of municipal solid waste. Food waste characteristics, such as high moisture and oil content, and variable composition, make it difficult to manage with conventional waste treatment methods. Part of food waste is disposed of in landfills, generating greenhouse gases and significantly increasing the carbon footprint. Various treatment methods such as composting and anaerobic digestion have been employed to treat and manage the remaining waste efficiently. This study provides an overview of the impact of composting as a food waste treatment method in Canada and paves way for the research of the usefulness of composting in addition to other food waste treatment methods such as anaerobic digestion. Average composting data for Canada was used to determine the change in the carbon footprint by the diversion of food waste using CCaLC2 software. It was determined that the overall carbon footprint of 1.38 and 1.33 mega-tons of CO2 was reduced from the composting of food waste in the years 2014 and 2016, which were approximately 18% and 20% of the total footprint of Canada municipal solid waste, respectively. The carbon footprint data collected herein were compared to the data from England, Sweden, and the USA to reveal the high effectiveness of composting in Canada.
Molecular Characterization of a Stable and Robust L-Asparaginase from Pseudomonas sp. PCH199: Evaluation of Cytotoxicity and Acrylamide Mitigation Potential
Authors: Kumar Subhash , Darnal Sanyukta , Patial Vijeta , Kumar Virender , Singh Dharam
Published: October 2022

L-asparaginase is an important industrial enzyme widely used to treat acute lymphoblastic leukemia (ALL) and to reduce acrylamide formation in food products. In the current study, a stable and robust L-asparaginase from Pseudomonas sp. PCH199, with a high affinity for L-asparagine, was cloned and expressed in Escherichia coli BL21(DE3). Recombinant L-asparaginase (Pg-ASNase II) was purified with a monomer size of 37.0 kDa and a native size of 148.0 kDa. During characterization, Pg-ASNase II exhibited 75.8 ± 3.84 U/mg specific activities in 50.0 mM Tris-HCl buffer (pH 8.5) at 50 °C. However, it retained 80 and 70% enzyme activity at 37 °C and 50 °C after 60 min, respectively. The half-life and kd values were 625.15 min and 1.10 × 10−3 min−1 at 37 °C. The kinetic constant Km, Vmax, kcat, and kcat/Km values were 0.57 mM, 71.42 U/mg, 43.34 s−1, and 77.90 ± 9.81 s−1 mM−1 for L-asparagine, respectively. In addition, the enzyme has shown stability in the presence of most metal ions and protein-modifying agents. Pg-ASNase II was cytotoxic towards the MCF-7 cell line (breast cancer) with an estimated IC50 value of 0.169 U/mL in 24 h. Further, Pg-ASNase II treatment led to a 70% acrylamide reduction in baked foods. These findings suggest the potential of Pg-ASNase II in therapeutics and the food industry.
Anti-Influenza Virus Potential of Probiotic Strain Lactoplantibacillus plantarum YML015 Isolated from Korean Fermented Vegetable
Authors: Majumder Rajib , Alam Md Badrul , Paudel Keshav Raj , Ahmed Khandaker Asif , Devkota Hari Prasad , Lee Sang-Han , Hansbro Philip M. , Park Yong-Ha
Published: October 2022

Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic Lactobacilli strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate its anti-viral potential against influenza virus A (IFVA) H1N1 using the MDCK cell line in vitro, and in embryonated eggs in ovo. The YML015 strain was selected from among the 1200 Lactobacilli isolates for further studies based on its potent anti-viral efficacy. YML015 was identified and characterized as Lactoplantibacillus plantarum YML015 based on the 16S rRNA gene sequencing and biochemically with an API 50 CHL Kit. In ovo assay experienced with embryonated eggs and the hemagglutination inhibition method, as well as cytopathogenic reduction assay, was performed individually to observe anti-influenza viral activity of YML015 against influenza virus A H1N1. Additionally, YML015 was classified for its non-resistance nature as safe for humans and animals as confirmed by the antibiotic susceptibility (MIC) test, cell viability, and hemolysis assay. The heat stability test was also experienced by using different heat-treated cell-free supernatant (CFS) samples of YML015. As a result, YML015 showed highly potent anti-viral activity against influenza virus A H1N1 in vitro in the MDCK cell line. Overall findings suggest that anti-influenza viral activity of L. plantarum YML015 makes it a potential candidate of choice for use as an influential probiotic in pharmacological preparations to protect humans and animals from flu and viral infection.
Characterization of Physicochemical Properties of Melanin Produced by Gluconobacter oxydans FBFS 97
Authors: Noman Abeer Essam , Al-Barha Naif S. , Chen Fusheng
Published: October 2022

The present study aimed to characterize melanin pigment extracted from Gluconobacter oxydans FBFS 97. After 14 days of culture at 28 °C in GY (glucose and yeast extract) liquid-state medium, G. oxydans FBFS97 produce the maximum melanin, up to about 12–15 mg/L. The physicochemical characteristics of the extracted melanin showed an ability to dissolve in 1 mol/L NaOH or 1 mol/L KOH, and insolubility in water and most organic solvents, such as chloroform and petroleum ether. The extracted melanin was confirmed to be exact melanin by ultraviolet-visible spectrophotometry, Fourier-transform infrared spectroscopy, thin-layer chromatography, elemental analysis, and scanning electron microscopy. The UV-visible spectrum of G. oxydans FBFS97 exhibited a maximum absorption peak at 230 nm. Extracted melanin demonstrated significant free radical-scavenging activity by DPPH and ABTS methods. The IC50 value of the extracted melanin for scavenging 50% DPPH radicals was 36.94 μg/mL, and the IC50 value of antioxidant activity for ABTS was 4.06 μg/mL. Hence, G. oxydans FBFS97 has the potential to be a new candidate for melanin production.
Fermented Total Mixed Ration with Cottonseed Meal or Rapeseed Meal Improved Growth Performance and Meat Quality of Hu Lamb Compared to Total Mixed Ration with Soybean Meal
Authors: Yusuf Hassan Ali , Rehemujiang Halidai , Ma Tao , Piao Minyu , Huo Ruiying , Tu Yan
Published: October 2022

Protein sources in livestock feed include cottonseed meals (CSM) or rapeseed meals (RSM). However, their use in feed diets is restricted due to anti-nutritional elements such as free gossypol or glucosinolate. The main objective of this study was to determine the effect of microbial fermentation of total mixed ration (TMR) with CSM/RSM on the growth performance and meat quality of Hu lamb. Fifty-one male Hu lambs (4 months old) with a 22.51 kg body weight were randomly assigned to three treatments and fed unfermented TMR with soybean meal (control group), FTMR (fermented total mixed ration) with CSM or RSM, respectively. The experiment lasted 97 days. Growth performance, rumen fermentation, and meat quality were measured. Overall dry matter intake (DMI), average daily gain (ADG), and final body weight (FBW) were similar among treatments (p > 0.05). There were no significant differences in the lightness (L*), redness (a*), and yellowness (b*) of longissimus thoracis (LT) muscle meat. There were no significant differences between the FTMR-CSM/RSM and control groups in drip loss, cooking loss, cooking percentage rate, or shear force of LT muscle meat. Lambs fed FTMR-CSM had significantly higher rumen fluid total volatile fatty acid values than those in control or FTMR-RSM groups (p < 0.05). Meat from lambs fed FTMR-RSM had a higher level of saturated fatty acids than lambs fed the control diet. The LT muscle meat of lamb fed FTMR-CSM had more unsaturated fatty acids than the control group (p < 0.001). In conclusion, FTMR with cottonseed or rapeseed meal can be fed without causing any adverse effects on Hu lamb.
Angular Momentum of Leaky Modes in Hollow-Core Fibers
Authors: Alagashev Grigory , Stafeev Sergey , Kotlyar Victor , Pryamikov Andrey
Published: October 2022

It is known that angular momentum (AM) is an important characteristic of light, which can be separated into the spin (SAM) and orbital parts (OAM). The dynamical properties of the spin and orbital angular momentums are determined by the polarization and spatial degrees of freedom of light. In addition to optical vortex beams possessing spatial polarization and phase singularities, optical fibers can be used to generate and propagate optical modes with the orbital and spin parts of the angular momentum. In this paper, using the example of hollow-core fibers, we demonstrate the fact that their leaky air core modes also have an orbital part of AM in the case of circular polarization arising from the spin–orbit interaction of the air core modes. The reason for the appearance of AM is the leakage of the air core mode energy.
Assessment and Mitigation of the Fire Vulnerability and Risk in the Historic City Centre of Aveiro, Portugal
Authors: Silva Dener , Rodrigues Hugo , Ferreira Tiago Miguel
Published: October 2022

Identifying fire risk in urban centres is instrumental for supporting informed decision-making and outlining efficient vulnerability mitigation strategies. Historic centres are particularly complex in this regard due to the high density of combustible materials in these areas, the favourable fire propagation conditions between buildings, and the complex urban morphology, which makes the evacuation of inhabitants difficult in case of a fire emergency. Recent safety regulations tend not to be fully applicable to historic city centres, where the specificities of the buildings, together with the need to safeguard their heritage value, make the rules for new buildings incompatible. For that reason, an adaptation of current evaluation methods is required to assure the safety of these places. The present paper aims to contribute to this topic by presenting and discussing the results obtained from the application of a simplified fire risk assessment methodology to a representative part of the historic city centre of Aveiro, Portugal. Data were collected through fieldwork building inspections and the results were mapped using a Geographic Information System tool. The study reveals that around 63% of the assessed buildings have a level of fire risk greater than the level of risk which is acceptable for buildings with this type of use and value. Based on the work developed, different mitigation strategies are suggested and compared. Finally, the results obtained in this work are compared with results published for historic urban areas with similar characteristics.
Nanotechnology in Residential Building Materials for Better Fire Protection and Life Safety Outcomes
Authors: Mullins-Jaime Charmaine , Smith Todd D.
Published: October 2022

Residential fires are the main source of fire deaths and injuries both in the United States and globally. As such, better fire-resistant building materials are needed to bolster fire protection and to enhance life safety. This is during a time when fewer materials are being used to construct homes. Nanotechnology may be a solution if it can overcome its current barriers to widespread adoption in residential construction, namely economy, sustainability, and safety. This research effort includes a critical examination of the literature from a safety perspective to address fire deaths and prevent personal injuries and illnesses by targeting fortification of residential construction building materials via the use of nanotechnology. The paper reviews nanotechnology for building materials by material type, known toxicity of various nanomaterials used in construction, and a discussion on a way forward through assessing materials by their ability to satisfy the requirements of sustainability, economy, and safety- both as a material designed to reduce fire injury and death and from a toxicological hazard perspective.
The Effect of Knocked-Down Anti-Müllerian Hormone mRNA on Reproductive Characters of Male Nile Tilapia (Oreochromis niloticus) through Inhibition of the TGF-Beta Signaling Pathway
Authors: Yan Yue , Tao Yifan , Cao Zheming , Lu Siqi , Xu Pao , Qiang Jun
Published: October 2022

Anti-Müllerian hormone (amh), an important regulator of gonad development in male teleosts, regulates the development and differentiation of germ cells. We performed transcriptional knock-down of amh in Nile tilapia (Oreochromis niloticus) using antisense RNA technology, resulting in down-regulation in the expression of amh transcription and Amh protein in males. Compared with the control groups, the fish in treatment groups with down-regulated amh had increased weight and an extremely significant decrease in the gonadosomatic index. Hematoxylin–eosin staining revealed impaired testis development and significant reductions in numbers of sperm. Serum estradiol levels were significantly increased, and the levels of testosterone, luteinizing hormone, and follicle-stimulating hormone were significantly decreased. RNA-sequencing analysis of the fish in the down-regulated amh and control groups identified 12,048 differentially expressed genes, of which 1281 were up-regulated and 10,767 were down-regulated. Kyoto Encyclopedia of Genes and Genomes analysis revealed that differentially expressed genes related to growth and development were mainly enriched in the Cell cycle, Endocytosis, TGF-beta signaling pathway, Wnt signaling pathway, FoxO signaling pathway, Insulin signaling pathway, and MAPK signaling pathway. The RNA-sequencing data accuracy was verified by qRT-PCR analysis of the expression levels of selected differentially expressed genes. The abnormal TGF-beta signaling pathway may cause fish weight gain, testis dysplasia, and abnormal spermatogenesis: smad5, smad3a, tgfb2, tgfbr1b, gsdf, and amh were significantly down-regulated. These findings indicated that antisense RNA technology has strong application prospects and can specifically knock down amh in Nile tilapia, resulting in an abnormal TGF-beta signaling pathway, inhibiting testis development and inducing weight gain.
Larval Retention and Homing Behaviour Shape the Genetic Structure of the Bullet Tuna (Auxis rochei) in the Mediterranean Sea
Authors: Ollé-Vilanova Judith , Pérez-Bielsa Núria , Araguas Rosa Maria , Sanz Núria , Saber Sámar , Macías David , Viñas Jordi
Published: October 2022

Background: The bullet tuna (Auxis rochei) is an epipelagic fish with a worldwide distribution that is highly targeted by fisheries. Genetic diversity and population genetics are good indicators of population structure and thus, essential tools for fisheries management. Knowing which factors (biotic and abiotic) might be shaping such structure is crucial for management plans. In the present study, we assessed the population structure of the bullet tuna in the western and central Mediterranean Sea. Methods: We used two types of genetic data: the mitochondrial DNA control region and seven microsatellite loci. The analysis of 431 sequences with a length of 386 bp from the mtDNA CR and the results from 276 individuals were genotyped by seven microsatellite loci. Results: Both types of markers coincided in showing significant genetic differences between an Iberian Peninsula–Strait of Gibraltar stock in comparison with a North African stock. Conclusions: We argue that this differentiation pattern is likely caused by reproductive strategies such as coastal spawning, larval retention, and natal homing behavior. These results should endorse the implementation of management plans for a resource that currently is not being managed. Thus, to ensure sustainability, these new policies should consider the presence of at least two genetically identified stocks.
Study of the Quality and Nutritional Value of Alosa sapidissima in the Postmortem Process
Authors: Li Le , Zhu Haojun , Yi Xiangyu , Nie Zhijuan , Zheng Yao , Yang Xiwei , Xu Pao , Yu Yaqing , Xu Gangchun
Published: October 2022

The American shad (Alosa sapidissima) is an important freshwater food fish, yet little is known about its postmortem quality. We sampled the meat of American shad and tracked the changes in color, pH, shear stress, cooking loss and total volatile basic nitrogen (TVB-N) over 48 h of storage at 24 °C (unrefrigerated) or 4 °C (refrigerated). Thereafter, the essential nutrients, fatty acids, hydrolyzed amino acids, free amino acids, and electronic tongue were evaluated. The results show that the L* and a* values decreased as storage progressed, while the b* value increased. The shear force decreased, but cooking losses in the American shad increased; the TVB-N value continuously increased over the duration of storage. The TVB-N content deviated from the freshness range at 48 h when stored at 24 °C. At 24 °C, the sweet amino acids reached a maximum at 6 h, and the bitter amino acids reached a maximum at 48 h. At 4 °C, there was a significant difference in bitter free amino acids at 48 h (p < 0.05). The nutrient composition showed that the contents of fat and protein decreased, whereas the water content increased. These results showed that American shad should be eaten within 6 h when stored at 24 °C, while American shad stored at 4 °C can maintain freshness for 24 h to obtain better product quality.
Evaluation of RANS-DEM and LES-DEM Methods in OpenFOAM for Simulation of Particle-Laden Turbulent Flows
Authors: Jaiswal Atul , Bui Minh Duc , Rutschmann Peter
Published: October 2022

CFD-DEM modelling of particle-laden turbulent flow is challenging in terms of the required and obtained CFD resolution, heavy DEM computations, and the limitations of the method. Here, we assess the efficiency of a particle-tracking solver in OpenFOAM with RANS-DEM and LES-DEM approaches under the unresolved CFD-DEM framework. Furthermore, we investigate aspects of the unresolved CFD-DEM method with regard to the coupling regime, particle boundary condition and turbulence modelling. Applying one-way and two-way coupling to our RANS-DEM simulations demonstrates that it is sufficient to include one-way coupling when the particle concentration is small (O ~ 10−5). Moreover, our study suggests an approach to estimate the particle boundary condition for cases when data is unavailable. In contrast to what has been previously reported for the adopted case, our RANS-DEM results demonstrate that simple dispersion models considerably underpredict particle dispersion and previously observed reasonable particle dispersion were due to an error in the numerical setup rather than the used dispersion model claiming to include turbulence effects on particle trajectories. LES-DEM may restrict extreme mesh refinement, and, under such scenarios, dynamic LES turbulence models seem to overcome the poor performance of static LES turbulence models. Sub-grade scale effects cannot be neglected when using coarse mesh resolution in LES-DEM and must be recovered with efficient modelling approaches to predict accurate particle dispersion.
The Effect of Ferulic Acid-Grafted Chitosan (FA-g-CS) on the Transmembrane Transport of Anthocyanins by sGLT1 and GLUT2
Authors: Ma Yi , Chen Xiaojiao , Diao Tiwei , Leng Yinjiang , Lai Xiaoqin , Wei Xin
Published: October 2022

This work aims to evaluate the effect of ferulic acid-grafted chitosan (FA-g-CS) on the interaction between anthocyanin (ANC) and sGLT1/GLUT2 and their functions in ANC transmembrane transport using Caco-2 cells. The transmembrane transport experiments of ANC showed its low transport efficiency (Papp < 10−6 cm/s), whereas the phenomenon of a significantly rise in anthocyanins transport efficiency was observed with the incubation of FA-g-CS (p < 0.05). In order to investigate the mechanism of FA-g-CS improving ANC transmembrane transport, Caco-2 cells were transfected with small interfering RNA (siRNA) specific for transporters sGLT1 and GLUT2, and incubated with ANC, FA-g-CS, or their combination. Subsequently, Western blot analyses and immunofluorescence staining were carried out to monitor the intracellular sGLT1 and GLUT2 levels. These siRNA-transfected cells, incubated with compounds, indicate that sGLT1 and GLUT2 participated in the ANC transmembrane transport and that FA-g-CS, ANC, or their combination enhance sGLT1/GLUT2 expression. In particular, Caco-2 cells incubated with both FA-g-CS and ANC show significantly increased sGLT1 or GLUT2 expression (>80%) compared with exclusively using FA-g-CS or ANC (<60%). Molecular docking results demonstrate that there is a good binding between FA-g-CS/ANC and sGLT1 or GLUT2. These results highlight that FA-g-CS promotes the transmembrane transport of ANC by influencing the interaction between ANC and sGLT1/GLUT2; the interaction between FA-g-CS and ANC could be another key factor that improves the bioavailability of ANC.
Heterologous Expression of the Lactobacillus sakei Multiple Copper Oxidase to Degrade Histamine and Tyramine at Different Environmental Conditions
Authors: Wang Xiaofu , Zhao Yunsong , Zhang Sufang , Lin Xinping , Liang Huipeng , Chen Yingxi , Ji Chaofan
Published: October 2022

Biogenic amines (BAs) are produced by microbial decarboxylation in various foods. Histamine and tyramine are recognized as the most toxic of all BAs. Applying degrading amine enzymes such as multicopper oxidase (MCO) is considered an effective method to reduce BAs in food systems. This study analyzed the characterization of heterologously expressed MCO from L. sakei LS. Towards the typical substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the optimal temperature and pH for recombinant MCO (rMCO) were 25 °C and 3.0, respectively, with the specific enzyme activity of 1.27 U/mg. Then, the effect of different environmental factors on the degrading activity of MCO towards two kinds of BAs was investigated. The degradation activity of rMCO is independent of exogenous copper and mediators. Additionally, the oxidation ability of rMCO was improved for histamine and tyramine with an increased NaCl concentration. Several food matrices could influence the amine-oxidizing activity of rMCO. Although the histamine-degrading activities of rMCO were affected, this enzyme reached a degradation rate of 28.1% in the presence of surimi. Grape juice improved the tyramine degradation activity of rMCO by up to 31.18%. These characteristics of rMCO indicate that this enzyme would be a good candidate for degrading toxic biogenic amines in food systems.
Understanding the Performance of Plant Protein Concentrates as Partial Meat Substitutes in Hybrid Meat Emulsions
Authors: Santos Mirian dos , Rocha Daniela Almeida Vieira Fogaça da , Bernardinelli Oigres Daniel , Oliveira Júnior Fernando Divino , de Sousa Diógenes Gomes , Sabadini Edvaldo , da Cunha Rosiane Lopes , Trindade Marco Antonio , Pollonio Marise Aparecida Rodrigues
Published: October 2022

Hybrid meat products are an excellent strategy to incorporate plant proteins into traditional meat formulations considering recent market trends focusing on the partial reduction in red meat content. In this work, we evaluated the effects of different concentrated plant proteins (soy, pea, fava bean, rice, and sunflower) in partially replacing meat in meat emulsion model systems. Soy, pea, and sunflower proteins showed great compatibility with the meat matrix, giving excellent emulsion stability and a cohesive protein network with good fat distribution. Otherwise, adding rice and fava bean proteins resulted in poor emulsion stability. Color parameters were affected by the intrinsic color of plant proteins and due to the reduction in myoglobin content. Both viscoelastic moduli, G′ and G″ decreased with the incorporation of plant proteins, especially for rice and fava bean. The temperature sweep showed that myosin denaturation was the dominant effect on the G′ increase. The water mobility was affected by plant proteins and the proportion between immobilized and intermyofibrillar water was quite different among treatments, especially those with fava bean and rice proteins. In vitro protein digestibility was lower for hybrid meat emulsion elaborated with rice protein. It is concluded that soy, pea, and mainly sunflower proteins have suitable compatibility with the meat matrix in emulsified products.
Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee
Authors: Akaeze Onyekachukwu , Kilonzo-Nthenge Agnes , Nandwani Dilip , Mafiz Abdullah Ibn , Nzomo Maureen , Aniume Tobenna
Published: October 2022

The production and consumption of organic fresh produce have constantly increased since the 1990s. Consumers prefer organic produce because it does not contain synthetic chemical residues that are often implicated in health problems. The contamination of fresh produce by pathogenic Enterobacteriaceae strains remains a major challenge, and is responsible for frequent foodborne disease outbreaks. The use of antibiotics has proved an effective treatment, but the increase in occurrences of antibiotic resistance is becoming a health challenge. This study seeks to establish the presence of antimicrobial resistance in Enterobacteriaceae on organic and conventional watermelon fruits. Watermelons used for this study were cultivated at the Tennessee State University Certified Organic Farm, Nashville. At harvest, nine fruits were selected from among fruits lying on plastic mulch, and nine from fruits lying on the soil of both organic and conventional plots. These were placed in sterile sample bags for microbial analysis. Spread plating technique, API 20E, and apiweb software were used for microbial isolation and identification. Identified strains were tested for antimicrobial resistance against 12 common antibiotics. Seventeen Enterobacteriaceae strains were isolated and identified. Isolates were susceptible to gentamycin, ciprofloxacin, and chloramphenicol, but were resistant to cefoxitin. Citrobacter freundii showed a 14.3% resistance to Streptomycin. Pantoea spp. and Providencia rettigeri showed 50% and 100% resistance to tetracycline. Findings from this study confirm the presence of antibiotic-resistant Enterobacteriaceae strains on organic watermelons in Nashville, TN.
Chestnut Starch Nanocrystal Combined with Macadamia Protein Isolate to Stabilize Pickering Emulsions with Different Oils
Authors: Zheng Jingyi , Zhao Lei , Yi Junjie , Zhou Linyan , Cai Shengbao
Published: October 2022

This study investigated the formation and molecular interaction mechanism of chestnut starch nanocrystal (SNC)/macadamia protein isolate (MPI) complexes and their application in edible oil-in-water Pickering emulsion (PE). SNC/MPI complexes were characterized by scanning electron microscopy and particle size analyzer. The PEs stabilized by SNC/MPI complexes were characterized by confocal laser scanning microscopy and rheological measurement. The results showed that hydrogen bonds between the two particles significantly affected the secondary structure and assembly of SNC/MPI complexes at the oil/water interface. The optimal mass ratio of SNC to MPI in the complexes with the best stability was determined as 20:1. The formation of edible oil-in-water PEs stabilized by SNC/MPI complexes significantly improved the oxidative and storage stability of different edible oils (olive oil, walnut oil, edible tea oil, and macadamia oil). These different edible oil-in-water PEs stabilized by SNC/MPI could be used as effective carriers of quercetin with their loading rates higher than 93%.
Food Security in China: A Brief View of Rice Production in Recent 20 Years
Authors: Tang Ling , Risalat Hamdulla , Cao Rong , Hu Qinan , Pan Xiaoya , Hu Yaxin , Zhang Guoyou
Published: October 2022

Rice production affects the food security and socioeconomic status of over half the world’s population. Rice-producing countries, however, are facing population growth, reduction in rice planting area, and global change. Understanding the trends of rice production and major determinants is key to regulating rice production. We thus analyzed the trends of rice production and related determinants in China from 2001 to 2021, revealing that the annual rice production (TRP) has risen steadily (r = 0.929, p < 0.0001) in recent 20 years. TRP in 2021 was 19.9% higher than that in 2001, which was primarily achieved by the increment of middle rice production (MRP). MRP increased by 46.2% from 2000 to 2018, and grain yield per unit area (GPA) was the largest in middle rice. The enhancement of GPAs is significantly correlated with the consumption of agricultural resources and the number of released rice cultivars, but variations exist. TRP and GPA vary in different provinces; Hunan (25 ± 2 megatons) and Xinjiang (8364 ± 806 kg/hectare) show the largest values, respectively. TRP could be further increased by 13.8% by improving MRP. The results suggest that rice production in China has a large potential to be further improved through regulations.
Nutritional Value and Physicochemical Characteristics of Alternative Protein for Meat and Dairy—A Review
Authors: Zeng Yan , Chen Enhui , Zhang Xuewen , Li Demao , Wang Qinhong , Sun Yuanxia
Published: October 2022

In order to alleviate the pressure on environmental resources faced by meat and dairy production and to satisfy the increasing demands of consumers for food safety and health, alternative proteins have drawn considerable attention in the food industry. However, despite the successive reports of alternative protein food, the processing and application foundation of alternative proteins for meat and dairy is still weak. This paper summarizes the nutritional composition and physicochemical characteristics of meat and dairy alternative proteins from four sources: plant proteins, fungal proteins, algal proteins and insect proteins. The difference between these alternative proteins to animal proteins, the effects of their structural features and environmental conditions on their properties, as well as the corresponding mechanism are compared and discussed. Though fungal proteins, algal proteins and insect proteins have shown some advantages over traditional plant proteins, such as the comparable protein content of insect proteins to meat, the better digestibility of fungal proteins and the better foaming properties of algal proteins, there is still a big gap between alternative proteins and meat and dairy proteins. In addition to needing to provide amino acid composition and digestibility similar to animal proteins, alternative proteins also face challenges such as maintaining good solubility and emulsion properties. Their nutritional and physicochemical properties still need thorough investigation, and for commercial application, it is important to develop and optimize industrial technology in alternative protein separation and modification.
Dissipation, Residue and Dietary Intake Risk Assessment of Penthiopyrad in Eggplants and Its Removal Using Various Household Processing Techniques
Authors: Dou Li , Mu Shiyin , Yang Guangqian , Chang Jinming , Zhang Kankan
Published: October 2022

A field trial was conducted to illustrate the dissipation and residue and assess the dietary intake risk of penthiopyrad in eggplants, and the distribution was further estimated after different household processing methods. Penthiopyrad dissipated quickly in eggplants, with half-lives of 1.85–2.56 days. The final residue data indicated that following the recommended spraying method, penthiopyrad would not threaten human health. Risk quotient results (<<100%) also demonstrated that the dietary intake risk of penthiopyrad in eggplants for Chinese consumers could be negligible. Washing, peeling and thermal treatments had significant removal effects on penthiopyrad from eggplants (0 < processing factor < 0.60). The characterization of the dissipation and distribution of penthiopyrad in field and processed eggplant samples could provide a more realistic reference for risk assessment of processed products, as well as some information for humans who may be exposed to penthiopyrad.
Improvement in Emulsifying Capacity of Goose Liver Protein Treated by pH Shifting with Addition of Sodium Tripolyphosphate and Its Proteomics Analysis
Authors: Zhang Yulong , Bai Yun , Yu Xiaobo , Li Zhen , Wang Peng , Xu Xinglian
Published: October 2022

Goose liver isolate treated by pH shifting and pH shifting/non-enzyme phosphorylation with goose liver isolate was used as a control. The functional property differences in the protein and proteins involved in the interfacial layer treated with pH shifting and non-enzyme phosphorylation were studied. Compared with the goose protein isolates (GPIs) at pH 7.0, the GPIs treated by pH shifting was not a good choice to be an emulsifier in a neutral environment, and non-enzyme phosphorylation inhibited the negative effects of pH shifting treatment and improved protein properties. The results of proteomics showed that the identified proteins in the interfacial layer belong to hydrophilic proteins. Non-enzyme phosphorylation increased the abundances of most proteins due to ion strength, including some phosphorylated proteins. Correlation analysis indicated that protein solubility was highly positively related with S0, intrinsic fluorescence, total sulfhydryl, free sulfhydryl, A0A0K1R5T3, R0KA48, R0KFP7, U3J1L1, P01989, R0JSM9, and R0LAD1, and was also highly negatively related with particle size and R0M210, R0M714, and R0LFA3. The emulsifying activity index (EAI) demonstrated highly positive correlation with protein solubility, and was correlated with R0JKI4, R0KK84, R0L1Y3, R0LCM7, A0A068C605, and U3IW62.
A Simple Aptamer SERS Sensor Based on Mesoporous Silica for the Detection of Chlorpyrifos
Authors: Dong Sa , Shi Qiuyun , He Kangli , Wu Jianwei , Zhu Zixin , Feng Jianguo
Published: October 2022

Chlorpyrifos is an organophosphorus insecticide, which can be used to control a variety of chewing and piercing mouthparts pests in agricultural production. It can destroy the normal nerve impulse conduction by inhibiting the activity of acetylcholinesterase or cholinesterase in the nerves, causing a series of poisoning symptoms. In order to achieve the quantitative analysis of chlorpyrifos residues in agricultural products, an aptamer-controlled signal molecule release method was developed in this study. The signal molecule 4-ATP of surface-enhanced Raman spectroscopy (SERS) was loaded into aminated mesoporous silica nanoparticles (MSNs-NH2) prepared by the one pot method, and then coated with an aptamer of chlorpyrifos through electrostatic interaction. The specific binding of the aptamer and chlorpyrifos led to the release of 4-ATP, and the amount of 4-ATP released was positively correlated with the amount of chlorpyrifos. Finally, the standard curve of chlorpyrifos quantitative detection based on SERS was established. Meanwhile, Ag-carrying mesoporous silica (Ag@MSNs) was prepared as the reinforcement substrate for SERS detection. The results showed that there was a good linear correlation between the Raman intensity and the concentration of chlorpyrifos at 25–250 ng/mL, and the limit of detection (LOD) was 19.87 ng/mL. The recoveries of chlorpyrifos in the apple and tomato samples were 90.08–102.2%, with RSD < 3.32%. This method has high sensitivity, specificity, reproducibility and stability, and can be used for the quantitative detection of chlorpyrifos in the environment and agricultural products.
Study on the Drying Technology of Tomato Pulp with Phytoene, Phytofluene and Lycopene Retention as Inspection Indexes
Authors: Li Liying , Yang Cheng , Zhang Jian , Zhang Lianfu
Published: October 2022

The objective was to design a feasible drying method to increase the retention rates of phytoene (PT), phytofluene (PTF) and lycopene (LYC) in tomato powder. The method was to compare the effects of vacuum freeze-drying (FD), vacuum drying (VD) and hot-air drying (HAD) technologies on tomato pulp rich in PT, PTF and LYC. When dried by HAD, the retention rates of PT, PTF and LYC decreased significantly (p < 0.05) only when the water content decreased from 30% to 3.5%. When dried by VD, the temperatures had no significant effect on the retention rates, and only alkaline conditions (pH = 9), Fe3+ and Al3+ could significantly reduce the retention rates (p < 0.05). Therefore, a combined drying process (CDP) was designed: before the water content decreased to 50%, HD (60 °C) technology was used; then, the paste was dried via VD (80 °C, 0.08 MPa) technology till the water content reached 5 ± 2%; loading weight was 40 g (thinkness 5.70 mm) for each batch. Compared with VD alone, the CDP technology improved the retention rates of PT and LYC by 12% and 36%, respectively, while PTF decreased by only 6%.
Dynamics of Microbial Communities, Flavor, and Physicochemical Properties during Ziziphus jujube Vinegar Fermentation: Correlation between Microorganisms and Metabolites
Authors: Ruan Wei , Liu Junli , Li Pengliang , Zhao Wei , Zhang Aixia , Liu Songyan , Wang Zhixin , Liu Jingke
Published: October 2022

Jujube pulp separated from Ziziphus jujube is often discarded after processing, resulting in a serious waste of resources and environmental pollution. Herein, Ziziphus jujube pulp was used as a raw material for vinegar fermentation. To investigate the dynamic distribution of microorganisms and flavor substances in ZJV, correlations between environmental variables (e.g., total acid, reducing sugar, temperature) and flavor substances (organic acids, amino acids, volatile substances) and microorganisms were analyzed. Physicochemical indicators (temperature, total acid, alcohol) were the main factors affecting ZJV fermentation. The middle and later stages of ZJV fermentation were the periods showing the largest accumulation of flavor substances. Organic acids (acetic acid, malic acid, citric acid, lactic acid), amino acids (Asp, Glu, Arg) and volatile substances (ethyl phenylacetate, phenethyl alcohol) were important odor-presenting substances in ZJV. In the bacterial community, the Operational Taxonomic Units (OTUs) with an average relative abundance of more than 10% in at least one fermentation stage were mainly Acetobacter, Lactobacillus and Saccharopolyspora, while it was Thermomyces in the fungal community. Pearson correlation coefficients showed that Penicillium, Lactobacillus and Acetobacter were the core microorganisms, implying that these microorganisms contributed to the flavor formation greatly in ZJV fermentation. This study reveals the correlation between physicochemical indexes and flavor substances and microorganisms in ZJV fermentation. The results of the study can provide a theoretical basis for the development of the ZJV industry.