bluuebunny/crossref_metadata_2025 huggingface dataset:
A Low-Power, Fast-Transient Output-Capacitorless LDO with Transient Enhancement Unit and Current Booster
Authors: Jiang Yongchao , Wang Lixin , Wang Shixin , Cui Mengyao , Zheng Zhuoxuan , Li Yuanzhe
Published: February 2022

With the wide application of advanced portable devices, output-capacitorless low dropout regulators (OCL-LDO) are receiving increasing attention. This paper presents a low quiescent current OCL-LDO with fast transient response. A transient enhancement unit (TEU) is proposed as the output voltage-spike detection circuit. It enhances the transient response by improving the slew-rate at the gate of the power transistor. In addition, a current booster (CB), which consists of a current subtractor and a non-linear current mirror, is designed to improve the slew-rate further. The current subtractor increases the transconductances of the differential-input transistors to obtain a large slewing current, while the non-linear current mirror further boosts the current with no extra quiescent current consumption. The simulated results show that the proposed OCL-LDO is capable of supplying 100 mA load current while consuming 10.3 μA quiescent current. It regulates the output at 1 V from a supply voltage ranging from 1.2 to 1.8 V. When the load current is stepped from 1 mA to 100 mA in 100 ns, the OCL-LDO has attained a settling time of 190 ns, and the output voltage undershoot and overshoot are controlled under 110 mV.
Optimization of CSP Plants with Thermal Energy Storage for Electricity Price Stability in Spot Markets
Authors: Borge-Diez David , Rosales-Asensio Enrique , Palmero-Marrero Ana I. , Acikkalp Emin
Published: February 2022

This research presents a novel optimization strategy for concentrating solar power (CSP) plants with thermal energy storage (TES) systems that aims to stabilize and reduce electricity prices in spot markets. In the current international scenario of initiatives with regulatory changes aiming to reduce climate change effects and therefore CO2 emissions, many countries are reducing the fossil fuel share in their respective electrical systems and increasing electrical renewable energy systems. These carbon free generation systems have inherent problems such as their intermittence which, combined with the lack of high-scale energy storage systems, cause a stability risk in electrical grid systems and require conventional fuel systems to match demand and production. In this research, we analyze spot price markets using a marginal price system that relies on natural gas and is quite sensitive to an increase in fuel prices, causing a direct increase in the final energy cost in systems with a high renewable energy share, and we study how optimally sized CSP plants with TES can be used as manageable solar energy systems and contribute to both price and grid stability. For the Spanish market, we analyze the ongoing market situation and generation mix and we present an optimization scheme using the System Advisor Model (SAM) software that aims to maximize energy availability throughout the day and contribute to peak generation. The results show that the optimization strategy increases the profitability of CSP plants but also ensures a contribution to spot price stability and reduction. A sensibility analysis is presented, and a case study is analyzed in southern Spain. The results show the importance of optimized CSP plants in an energy transition and a strategy is proposed that can be extended to other locations worldwide.
Analysis of Reject Water Formed in the Mechanical Dewatering Process of Digested Sludge Conditioned by Physical and Chemical Methods
Authors: Bień Beata , Bień Jurand D.
Published: February 2022

Reject water separated from digested sludge may be a potential source of nutrients due to its high content. However, most often, reject water after sludge dewatering is directed to sewage lines at wastewater treatment plants, negatively affecting their operation, especially in the biological part. The activities related to sludge conditioning before dewatering have a direct impact on the quality of the reject water. The reject water of raw digested sludge is characterized by very high concentrations of ammonium nitrogen, at 1718 mgN-NH4+/dm3; phosphates, at 122.4 mgPO43−/dm3; and chemical oxygen demand (COD), at 2240 mgO2/dm3. The objective of the research was to determine the impact of selected sludge conditioning methods on the quality of reject water obtained after sludge dewatering. The following parameters were analyzed in the reject water: the chemical oxygen demand (COD), phosphates, ammonium nitrogen, and total suspended solids (TSS). It has been observed that the sludge sonification process increases the content of impurities (COD, phosphates) in reject water with an increase in the amplitude of the ultrasonic field. On the other hand, the chemical reagents cause a decrease in the concentration of the pollutants with an increase of the chemical dose. It has been found that the inorganic coagulant PIX 113 gives much better results regarding the reduction of contamination than the polyelectrolyte Zetag 8180.
A Comparison of Frequency-Dependent Soil Models: Electromagnetic Transient Analysis of Overhead Transmission Lines Using Modal Decomposition
Authors: Garbelim Pascoalato Tainá Fernanda , Justo de Araújo Anderson Ricardo , Leon Colqui Jaimis Sajid , Kurokawa Sérgio , Pissolato Filho José
Published: February 2022

This article investigates the influence of four causal frequency-dependent (FD) soil models and their impact on the responses of a multiphase overhead transmission line (OHTL) with ground wires, generated by a lightning strike. The FD models proposed by Longmire-Smith (LS), Messier (M), Portela (P) and Alípio-Visacro (AV) are considered. The ground-return impedance and admittance matrices are computed with the Nagakawa approach for both frequency-constant and FD soil models. The frequency-domain modal voltages and time-domain transient voltages are assessed in this work. Modal decomposition technique is used to study the attenuation constant, propagation velocity and voltages for each propagation mode. Simulations are carried out in a frequency range of 100 Hz to 10 MHz, for OHTLs with lengths of 1 and 10 km, on soils of 700 and 4000 Ω·m. Simulation results demonstrated that the Portela (P) model has resulted in more significant variation in the ground-return impedance and admittance, constant attenuation and propagation velocity in which a pronounced variation, especially at the high frequencies, is seen. On the other hand, Longmire-Smith (LS) and Messier (M) have produced similar results in both frequency and time domains. Additionally, the Alípio-Visacro (AV) model has produced intermediate responses, being the model recommended by CIGRÈ WG C4.33. Time-domain induced voltage waveforms obtained with the Portela (P) model has shown pronounced differences, especially at the peak values, for the high-resistive soil. This study demonstrates the importance of considering the FD soil models to assess the transient responses adequately, especially when OHTLs are on high-resistive soils.
Target Force Curve Searching Method for Axial Electromagnetic Dynamic Balance of Scroll Compressor
Authors: Qu Xiao , Shi Yantao , Cai Jiongjiong
Published: February 2022

In order to explore the axial electromagnetic dynamic balance force curve of a scroll compressor under the action of thermal energy, radial clearance, mechanism friction, and other factors in actual working conditions, an axial force exploration method that can automatically approach sections is proposed in this paper. Considering the dynamic response ability of the electromagnetic balance system, an automatic optimization algorithm of the partition number was proposed to find the optimal partition number in order to achieve the optimal tracking effect. An experimental platform was built to test the effect of the segmented tracking method on calibrating the deviation between the theoretical axial force curve and the real curve. The results show that the curve construction method proposed in this paper has convergence. This method can automatically and accurately construct the axial balance force curve required by the electromagnetic dynamic balance. Through the automatic optimization algorithm, the standard error (RMSE) between the target curve and the theoretical curve was reduced from 290 to 22.6, and the number of partitions with the lowest standard error was 20. The results provide a useful reference for the accurate, automatic, and efficient exploration of the actual axial sealing force of the scroll compressor.
Surrogate Model of the Optimum Global Battery Pack Thermal Management System Control
Authors: Arrinda Mikel , Vertiz Gorka , Sanchéz Denis , Makibar Aitor , Macicior Haritz
Published: February 2022

The control of the battery-thermal-management-system (BTMS) is key to prevent catastrophic events and to ensure long lifespans of the batteries. Nonetheless, to achieve a high-quality control of BTMS, several technical challenges must be faced: safe and homogeneous control in a multi element system with just one actuator, limited computational resources, and energy consumption restrictions. To address those challenges and restrictions, we propose a surrogate BTMS control model consisting of a classification machine-learning model that defines the optimum cooling-heating power of the actuator according to several temperature measurements. The la-belled-data required to build the control model is generated from a simulation environment that integrates model-predictive-control and linear optimization concepts. As a result, a controller that optimally controls the actuator with multi-input temperature signals in a multi-objective optimization problem is constructed. This paper benchmarks the response of the proposal using different classification machine-learning models and compares them with the responses of a state diagram controller and a PID controller. The results show that the proposed surrogate model has 35% less energy consumption than the evaluated state diagram, and 60% less energy consumption than a traditional PID controller, while dealing with multi-input and multi-objective systems.
Analysis and Tests of Lateral Resistance of Bolted and Screwed Connections of CLT
Authors: Huo Liangliang , Zhu Enchun , Niu Shuang , Wu Guofang
Published: February 2022

The lateral resistance of dowel-type connections with CLT is related to its lay-up, species of the laminations and even the manufacture method. Treating the CLT as homogeneous material, current methods develop new equations through test results or make use of the existing equations for the embedment strength already used in design codes; thus, the lateral resistance of dowel-type connections of CLT can be calculated. This kind of approach does not take the embedment stress distribution into account, which may lead to inaccuracy in predicting the lateral resistance and yield mode of the dowel-type connections in CLT. In this study, tests of the bolted connections and the screwed connections of CLT were conducted by considering the effects of the orientation of the laminations, the thickness of the connected members, the fastener diameter and strength of the materials. The material properties including yield strength of the fasteners and embedment strength of the CLT laminations were also tested. Using analysis of the dowel-type connections of CLT by introducing the equivalent embedment stress distribution, equations for the lateral resistance of the connections based on the European Yield Model were developed. The predicted lateral resistance and yield modes were in good agreement with the test results; the correctness and the feasibility of the equations were thus validated.
Antimicrobial Resistance and Virulence of Non-Typhoidal Salmonella from Retail Foods Marketed in Bangkok, Thailand
Authors: Kong-Ngoen Thida , Santajit Sirijan , Tunyong Witawat , Pumirat Pornpan , Sookrung Nitat , Chaicumpa Wanpen , Indrawattana Nitaya
Published: February 2022

Nontyphoidal-Salmonella bacteria cause foodborne gastroenteritis that may lead to fatal bacteremia, osteomyelitis, and meningitis if not treated properly. The emergence of multidrug-resistant Salmonella strains is a global public health threat. Regular monitoring of genotypes and phenotypes of Salmonella isolated from humans, animals, foods, and environments is mandatory for effective reduction and control of this food-borne pathogen. In this study, antimicrobial-resistant and virulent genotypes and phenotypes of Salmonella isolated from retail food samples in Bangkok, Thailand, were investigated. From 252 raw food samples, 58 Salmonella strains that belonged only to serotype Enteritidis were isolated. Disc diffusion method showed that all isolates were still sensitive to amikacin and carbapenems. More than 30% of the isolates were resistant to ampicillin, tetracycline, and ciprofloxacin. Twenty isolates resist at least three antibiotic classes. Minimum inhibitory concentration tests showed that 12.07% of the isolates produced extended-spectrum β-Lactamase. Polymerase chain reaction indicated that 32.76, 81.03, 39.66, and 5.17% of the isolates carried blaTEM-1, tetA, sul2, and dfrA7, respectively. All isolates were positive for invasion-associated genes. Effective prevention and control of Salmonella (as well as other food-borne pathogens) is possible by increasing public awareness and applying food hygienic practices. Active and well harmonised “One Health” co-operation is required to effectively control food-borne zoonosis.
Cylindrical Gravastar Like-Structures in f(G) Gravity
Authors: Bhatti M. Z. , Yousaf Z. , Rehman A.
Published: February 2022

The aim of this manuscript is to explore singularity-free solution for a specific self-gravitating highly dense object known as gravastar suggested by Mazur and Mottola, in the context of f(G) gravity theory. Gravastars are regarded as a possible alternate to black hole. To derive modified field equations and law of conservation related to Gauss-Bonnet gravity, we assume cylindrically symmetric irrotational configuration. Particular equation of states are used for the illustration of three sectors of gravastar model. Furthermore, we are intended to obtain a regular solution for our model and graphs will be used to elaborate various substantial characteristics of it.
Breast Tumour Kinase (Brk/PTK6) Contributes to Breast Tumour Xenograft Growth and Modulates Chemotherapeutic Responses In Vitro
Authors: Burmi Rajpal S. , Box Gary M. , Wazir Umar , Hussain Haroon A. , Davies Julie A. , Court William J. , Eccles Suzanne A. , Jiang Wen G. , Mokbel Kefah , Harvey Amanda J.
Published: February 2022

Breast tumour kinase (Brk/PTK6) is overexpressed in up to 86% of breast cancers and is associated with poorer patient outcomes. It is considered a potential therapeutic target in breast cancer, even though the full spectrum of its kinase activity is not known. This study investigated the role of the kinase domain in promoting tumour growth and its potential in sensitising triple negative breast cancer cells to standard of care chemotherapy. Triple negative human xenograft models revealed that both kinase-inactive and wild-type Brk promoted xenograft growth. Suppression of Brk activity in cells subsequently co-treated with the chemotherapy agents doxorubicin or paclitaxel resulted in an increased cell sensitivity to these agents. In triple negative breast cancer cell lines, the inhibition of Brk kinase activity augmented the effects of doxorubicin or paclitaxel. High expression of the alternatively spliced isoform, ALT-PTK6, resulted in improved patient outcomes. Our study is the first to show a role for kinase-inactive Brk in human breast tumour xenograft growth; therefore, it is unlikely that kinase inhibition of Brk, in isolation, would halt tumour growth in vivo. Breast cancer cell responses to chemotherapy in vitro were kinase-dependent, indicating that treatment with kinase inhibitors could be a fruitful avenue for combinatorial treatment. Of particular prognostic value is the ratio of ALT-PTK6:Brk expression in predicating patient outcomes.
Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11
Authors: Miyata Yugo , Tokumoto Shoko , Arai Tomohiko , Shaikhutdinov Nurislam , Deviatiiarov Ruslan , Fuse Hiroto , Gogoleva Natalia , Garushyants Sofya , Cherkasov Alexander , Ryabova Alina , Gazizova Guzel , Cornette Richard , Shagimardanova Elena , Gusev Oleg , Kikawada Takahiro
Published: February 2022

Genomic safe harbors (GSHs) provide ideal integration sites for generating transgenic organisms and cells and can be of great benefit in advancing the basic and applied biology of a particular species. Here we report the identification of GSHs in a dry-preservable insect cell line, Pv11, which derives from the sleeping chironomid, Polypedilum vanderplanki, and similar to the larvae of its progenitor species exhibits extreme desiccation tolerance. To identify GSHs, we carried out genome analysis of transgenic cell lines established by random integration of exogenous genes and found four candidate loci. Targeted knock-in was performed into these sites and the phenotypes of the resulting transgenic cell lines were examined. Precise integration was achieved for three candidate GSHs, and in all three cases integration did not alter the anhydrobiotic ability or the proliferation rate of the cell lines. We therefore suggest these genomic loci represent GSHs in Pv11 cells. Indeed, we successfully constructed a knock-in system and introduced an expression unit into one of these GSHs. We therefore identified several GSHs in Pv11 cells and developed a new technique for producing transgenic Pv11 cells without affecting the phenotype.
Predictive Simulation for Surface Fault Occurrence Using High-Performance Computing
Authors: Sawada Masataka , Haba Kazumoto , Hori Muneo
Published: February 2022

Numerical simulations based on continuum mechanics are promising methods for the estimation of surface fault displacements. We developed a parallel finite element method program to perform such simulations and applied the program to reproduce the 2016 Kumamoto earthquake, where surface rupture was observed. We constructed an analysis model of the 5 × 5 × 1 km domain, including primary and secondary faults, and inputted the slip distribution of the primary fault, which was obtained through inversion analysis and the elastic theory of dislocation. The simulated slips on the surface were in good agreement with the observations. We then conducted a predictive simulation by inputting the slip distributions of the primary fault, which were determined using a strong ground motion prediction method for an earthquake with a specified source fault. In this simulation, no surface slip was induced in the sub-faults. A large surface slip area must be established near a sub-fault to induce the occurrence of a slip on the surface.
Possible Effects on Health of Ultrasound Exposure, Risk Factors in the Work Environment and Occupational Safety Review
Authors: Moyano David Baeza , Paraiso Daniel Arranz , González-Lezcano Roberto Alonso
Published: February 2022

Ultrasonic waves are mechanical waves with a frequency greater than 20,000 Hz. Ultrasonic waves are emitted by devices that are used in industry or that have a medical or aesthetic purpose. There is growing interest in the effect of ultrasound absorption on the human body, since people’s exposure to these acoustic waves has increased considerably in recent years. There are more and more devices that emit ultrasounds used for different sanitary procedures, aesthetic treatments and industrial processes, creating more possibilities of ultrasound noise, and therefore an increased risk of occupational hazard and occupational danger. Experiments on animals have shown damage to internal organs from receiving different ultrasonic frequencies. The main task of this work was to organize and summarize recent studies on ultrasound to reflect the current state of this technique and establish a systematic basis for future lines of research. This work has allowed us to better understand the unknown field of these high frequencies of sound, and highlights the need to carry out more studies on the ultrasound emissions that can be absorbed by the human body to determine how this energy could affect humans by calculating the maximum dose of exposure and developing manuals for the use of ultrasound-emitting equipment to protect the health of workers and all people. It is necessary to develop regulations by public administrations to improve the protection of workers, health professionals, patients and all people in general for better occupational safety, indoor environmental quality and environmental health.
Association between Social Support and Physical Activity in Patients with Coronary Artery Disease: Multiple Mediating Roles of Self-Efficacy and Autonomous Motivation
Authors: Han Nam-Sin , Won Mi-Hwa
Published: February 2022

Physical inactivity in patients with coronary artery disease is linked to recurrent cardiac events. Given that social support may be an enduring major factor for physical activity, the mechanism underlying the multiple mediating effects of self-efficacy and autonomous motivation on the relationship between social support and physical activity in patients with coronary artery disease has hardly been examined. Therefore, this study aimed to clarify the multiple mediating roles of social support and physical activity on the relationship between self-efficacy and autonomous motivation in patients with coronary artery disease. This descriptive cross-sectional study included 190 inpatients who were diagnosed with coronary artery disease and admitted to a cardiology ward university hospital in Korea. Parallel multiple mediated models were tested using the SPSS PROCESS macro. The direct effects of social support on physical activity and the indirect effects of self-efficacy and autonomous motivation on social support and physical activity were statistically significant. Thus, positive social support from health-care providers has an important role to play in promoting physical activity by increasing self-efficacy and autonomous motivation for physical activity in patients with coronary artery disease.
COVID-19 in Patients with Transfusion Dependent Thalassemia (TDT) in Indonesia: Characteristics of the Disease and Patients, and Comparison between Epidemiological Data for COVID-19 and Thalassemia in Indonesia and Southeast Asia
Authors: Atmakusuma Tubagus Djumhana
Published: February 2022

Background: People living with transfusion dependent thalassemia have a high risk of becoming infected with COVID-19. This can be caused by both internal factors, namely the formation of alloantibodies and autoimmune disorder, and external factors such as routine visits for blood transfusions. Chronic complications of thalassemia also render them more vulnerable to infectious diseases, including COVID-19. However, anecdotal data shows that thalassemia patients experience less incidence of COVID-19 compared to the general population. Purpose: This study aims to find the correlation between COVID-19 in thalassemia-dependent transfusion patients in Indonesia and Southeast Asia. Patients and Methods: This study used a cross-sectional design. The study was conducted at the Division of Hematology and Medical Oncology of the Cipto Mangunkusumo Hospital in Jakarta from May 2020–August 2021. The total sampling method was used involving all thalassemia major patients who had been infected with COVID-19 (obtained directly from medical record and through the thalassemia patients-parents foundation). Results: From 10,397 patients with thalassemia, 67 (0.64%) people were infected by COVID-19 and 2 (2.9%) were deceased. Meanwhile, the incidence of COVID-19 in the general population of Indonesia was 0.87% (more than in the thalassemia population). This means that thalassemia might provide additional protection against COVID-19 due to several mechanisms. This phenomenon has also been seen in other countries with a high prevalence of thalassemia, wherein there are less COVID-19 cases despite the pandemic. On the contrary, countries with low rates of thalassemia had experienced deadly surges of the pandemic. Conclusion: Indonesia and other countries with a high prevalence of thalassemia have lower COVID-19 incidence than countries with low prevalence of thalassemia. Thalassemia might provide additional protection against COVID-19. Well-designed studies are needed to provide better evidence on the protective effect of thalassemia on COVID-19.
Choice, Motives, and Mixed Messages: A Qualitative Photo-Based Inquiry of Parents’ Perceptions of Food and Beverage Marketing to Children in Sport and Recreation Facilities
Authors: Prowse Rachel , Storey Kate , Olstad Dana Lee , Carson Valerie , Raine Kim D.
Published: February 2022

Unhealthy food and beverage availability and sponsorship undermine healthy food practices in sport and recreation. We conducted a focused ethnography with reflexive photo-interviewing to examine parents’ awareness, reactions, and experiences of food and beverage marketing in and around their children’s physical activity in public sport and recreation facilities. Eleven parents took photos of what they thought their facility was ‘saying about food and eating’. Photos guided semi-structured interviews on the ‘4Ps’ of marketing (product, pricing, placement, promotion). Thematic analysis was conducted by holistic coding followed by in vivo, versus, and value coding. Photo-taking increased parents’ awareness of food marketing in facilities. Reactions to food and beverage marketing were positive or negative depending on parents’ perspectives of healthy food availability (choice), marketers’ motives, and mixed messages within the facility. Parents experienced their children requesting ‘junk’ food at the facility leading to parents actively attempting to reduce the frequency of these requests. Healthy eating promotion in sport and recreation facilities was misaligned with the foods and beverages available which contributed to parents’ distrust of social marketing initiatives. Critically evaluating the alignment of commercial and social marketing in recreation and sport may help inform effective healthy eating interventions that are accepted and supported by parents.
Acute Physiological Response to Different Sprint Training Protocols in Normobaric Hypoxia
Authors: Maldonado-Rodriguez Naomi , Bentley David J. , Logan-Sprenger Heather M.
Published: February 2022

Background: the purpose of this study was to examine acute physiological responses to and the performance effects of two sprint training protocols in normobaric hypoxic conditions. Methods: Healthy competitive female (n = 2) and male (n = 5) kayakers (19 ± 2.1 years) performed four sprint training sessions on a kayak ergometer over a period of two weeks. Participants performed five sets of 12 × 5 s sprints or 3 × 20 s sprints in both normobaric normoxic (NOR, FiO2 = 20.9%) or normobaric hypoxic (HYP, FiO2 = 13.6%) conditions. The peak power output (PPO), rate of perceived exertion (RPE), and heart rate (HR) of each participant were monitored continuously. Their blood lactate concentrations ([BLa+]), in addition to their blood gas (mixed-venous partial pressure (p) of carbon dioxide (pCO2), O2 (pO2), and oxygen saturations (sO2)) were collected before and after exercise. Results: A significantly greater RPE, HR, and [BLa+] response and a significant decrease in pCO2, pO2, and sO2 were observed in HYP conditions versus NOR ones, independent of the type of training session. The PPO of participants did not differ between sessions. Their RPE in HYP12 × 5 was greater compared to all other sessions. Conclusions: The HYP conditions elicited significantly greater physiological strain compared to NOR conditions and this was similar in both training sessions. Our results suggest that either sprint training protocol in HYP conditions may induce more positive training adaptations compared to sprint training in NOR conditions.
Evaluation of Sociodemographic Factors and Prevalence of Oral Lesions in People Living with HIV from Cacoal, Rondônia, Amazon Region of Brazil
Authors: da Rocha Graziela de Carvalho Tavares , Fonseca Ricardo Roberto de Souza , Oliveira-Filho Aldemir Branco , Ribeiro Andre Luis Ribeiro , de Menezes Silvio Augusto Fernandes , Laurentino Rogério Valois , Machado Luiz Fernando Almeida
Published: February 2022

Background: It is necessary to evaluate and understand the prevalence and risk factors of oral lesions (OL) in people living with HIV (PLWH) who were never studied before. The present study aimed to describe the prevalence of OL and its correlation with CD4+ T lymphocytes counts and HIV plasma viral load in PLWH treated in Rondônia. Methods: A cross-sectional study was carried out at Cacoal city, Rondônia state, Northern Brazil. Sociodemographic, epidemiological, immunologic and virological information of 113 PLWH were collected from medical records and dental examination was conducted to diagnose and classify OL in PLWH. Statistical analysis was performed using relative frequency distribution, ANOVA, Kruskal–Wallis, T-student and Mann-Whitney tests. Results: The overall prevalence of oral lesions was 28.3% (32/113), with candidiasis (7/32; 21.8%) and aphthous ulcer (7/32; 21.8%) being the most prevalent. There was a predominance of females, most patients being married, with a low level of education, a family income of 1 to 3 minimum wages and a single partner. An association was observed between the presence of oral lesions and a high viral load, as well as a lower occurrence of oral lesions in individuals with a higher count of CD4+ T cells. Conclusions: This study reveals a low prevalence of OL among PLWH, as well as the absence of relationship between HIV viral load, CD4+ T cells count and OL high prevalence.
Let It Beat: How Lifestyle and Psychosocial Factors Affect the Risk of Sudden Cardiac Death—A 10-Year Follow-Up Study
Authors: Obrova Jana , Sovova Eliska , Ivanova Katerina , Furstova Jana , Taborsky Milos
Published: February 2022

(1) Background: The aim of this study was to evaluate the lifestyle and occurrence of psychosocial factors in patients with a high risk of sudden cardiac death (SCD) and to explore their effect on the occurrence of the adequate therapy of an Implantable Cardioverter Defibrillator (ICD). (2) Methods: In this retro-prospective single-centre study, a group of patients aged 18–65 years old, who underwent the first ICD implantation for primary (PP) or secondary (SP) prevention between 2010–2014, was studied. The control group consisted of pair-matched (age ± 5 years, gender) respondents without a high risk of SCD. Information was obtained using a self-reported questionnaire and hospital electronic health records. The adequacy of ICD therapy was evaluated regularly until 31 January 2020. Multivariate logistic regression models were employed to assess the risk of SCD. (3) Results: A family history of SCD, coronary artery disease, diabetes mellitus and depression significantly aggravated the odds of being at a high risk of SCD. The occurrence of an appropriate ICD therapy was significantly associated with being in the SP group, BMI, education level and TV/PC screen time. (4) Conclusions: Lifestyle and psychosocial factors have been confirmed to affect the risk of SCD. Early identification and treatment of coronary artery disease and its risk factors remain the cornerstones of preventive effort. Further research is needed to evaluate the complex nature of psychosocial determinants of cardiac health.
Active Video Games Improve Muscular Fitness and Motor Skills in Children with Overweight or Obesity
Authors: Comeras-Chueca Cristina , Villalba-Heredia Lorena , Perez-Lasierra Jose Luis , Marín-Puyalto Jorge , Lozano-Berges Gabriel , Matute-Llorente Ángel , Vicente-Rodríguez Germán , Gonzalez-Aguero Alex , Casajús José A.
Published: February 2022

(1) Background: Childhood obesity is an important public health problem. Children with overweight or obesity often tend to show the pediatric inactivity triad components; these involve exercise deficit disorder, pediatric dynapenia, and physical illiteracy. The aim of the study was to examine the influence of an active video games (AVG) intervention combined with multicomponent exercise on muscular fitness, physical activity (PA), and motor skills in children with overweight or obesity. (2) Methods: A total of 29 (13 girls) children (10.07 ± 0.84 years) with overweight or obesity were randomly allocated in the intervention group (AVG group; n = 21) or in the control group (CG; n = 8). The intervention group performed a 5-month AVG training using the Xbox 360® with the Kinect, the Nintendo Wii®, dance mats, and the BKOOL® interactive cycling simulator, combined with multicomponent exercise, performing three sessions per week. The control group continued their daily activities without modification. Weight, PA using accelerometers, and motor competence using the Test of Gross Motor Development 3rd edition were measured. Muscular fitness was evaluated through the Counter Movement Jump height, maximal isometric strength of knee extension and handgrip strength, and lean mass using Dual-energy X-ray Absorptiometry. Mann–Whitney U and Wilcoxon signed rank tests were performed. The biserial correlation coefficients (r) were calculated. Spearman’s correlation coefficients among PA, muscular fitness, and motor competence variables were also calculated. (3) Results: The AVG group significantly increased their knee extension maximal isometric strength (4.22 kg; p < 0.01), handgrip strength (1.93 kg; p < 0.01), and jump height (1.60 cm; p < 0.01), while the control group only increased the knee extension maximal isometric strength (3.15 kg; p < 0.01). The AVG group improved motor competence and light physical activity (p < 0.05) and decreased sedentary time (p < 0.05). Lean mass improved in both AVG group and CG (p < 0.05). Lastly, the percentage of improvement of motor skills positively correlated with the percentage of improvement in vigorous PA (r = 0.673; p = 0.003) and the percentage of improvement in CMJ (r = 0.466; p = 0.039). (4) Conclusions: A 5-month intervention combining AVG with multicomponent training seems to have positive effects on muscle fitness, motor competence, and PA in children with overweight or obesity.
A Non-Exercise Model for Predicting Cardiovascular Risks among Apparently Healthy Male Office Workers—Cross-Sectional Analysis: A Pilot Study
Authors: Zadarko Emilian , Zadarko-Domaradzka Maria , Barabasz Zbigniew , Sobolewski Marek
Published: February 2022

The health condition of working-age males in Poland remains largely associated with long-lasting sick leaves, one of the main reasons of which being cardiovascular diseases (CVD). The aim of this work was to develop a prediction model for FIT Treadmill Score (“FIT” refers to Henry Ford ExercIse Testing (FIT) Project) that only depends on easily accessible somatic data and smoking without the need to perform the exercise test anymore. The study comprised 146 men with a negative cardiological history, aged 26–60, with desk-jobs. By means of regression analysis it was tested to what degree obesity-related indices as well as smoking cigarettes allow for determining the measure level of mortality risk, without the necessity of performing an exercise test. The following independent variables were entered into the linear regression model: age, BMI, Fat%, waist circumference (WC), waist to height ratio (WHtR) as well as smoking. Statistically significant factors were singled out from among them. The obtained model accounts for a significant part (over 87%) of the variability of the mortality risk measure among the tested population. Based on the value of the standardised regression coefficient β, it can be stated that age is the factor that mostly determines the mortality risk measure, followed by the WHtR and smoking. The simplicity of the worked-out model and, resulting from it, the possibility of its common application should enable better health monitoring of working-age men with regard to cardiovascular disease occurrence and, related to it, mortality risk, thereby improving the quality of public health management.
Lipid Signaling Requires ROS Production to Elicit Actin Cytoskeleton Remodeling during Plant Innate Immunity
Authors: Cao Lingyan , Wang Wenyi , Zhang Weiwei , Staiger Christopher J.
Published: February 2022

In terrestrial plants a basal innate immune system, pattern-triggered immunity (PTI), has evolved to limit infection by diverse microbes. The remodeling of actin cytoskeletal arrays is now recognized as a key hallmark event during the rapid host cellular responses to pathogen attack. Several actin binding proteins have been demonstrated to fine tune the dynamics of actin filaments during this process. However, the upstream signals that stimulate actin remodeling during PTI signaling remain poorly characterized. Two second messengers, reactive oxygen species (ROS) and phosphatidic acid (PA), are elevated following pathogen perception or microbe-associated molecular pattern (MAMP) treatment, and the timing of signaling fluxes roughly correlates with actin cytoskeletal rearrangements. Here, we combined genetic analysis, chemical complementation experiments, and quantitative live-cell imaging experiments to test the role of these second messengers in actin remodeling and to order the signaling events during plant immunity. We demonstrated that PHOSPHOLIPASE Dβ (PLDβ) isoforms are necessary to elicit actin accumulation in response to flg22-associated PTI. Further, bacterial growth experiments and MAMP-induced apoplastic ROS production measurements revealed that PLDβ-generated PA acts upstream of ROS signaling to trigger actin remodeling through inhibition of CAPPING PROTEIN (CP) activity. Collectively, our results provide compelling evidence that PLDβ/PA functions upstream of RBOHD-mediated ROS production to elicit actin rearrangements during the innate immune response in Arabidopsis.
Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification
Authors: Song Min , Chang Jeong-Ho
Published: February 2022

Candida antarctica lipase B (CalB) enzyme was encapsulated and cross-linked by silica matrix to enhance its thermal stability and reusability, and demonstrated an enzymatic ability for rapid hydrolysis and esterification. Silica encapsulated CalB particles (Si-E-CPs) and silica cross-linked CalB particles (Si-CL-CPs) were prepared as a function of TEOS concentration. The particle size analysis, thermal stability, catalytic activity in different pHs, and reusability of Si-E-CPs and Si-CL-CPs were demonstrated. Furthermore, the determination of the CalB enzyme in Si-E-CPs and Si-CL-CPs was achieved by Bradford assay and TGA analysis. Enzymatic hydrolysis was performed against the p-nitrophenyl butyrate and the catalytic parameters (Km, Vmax, and Kcat) were calculated by the Michaelis–Menten equation and a Lineweaver–Burk plot. Moreover, enzymatic synthesis for benzyl benzoate was demonstrated by esterification with an acyl donor of benzoic acid and two acyl donors of benzoic anhydride. Although the conversion efficiency of Si-CL-CPs was not much higher than that of native CalB, it has an efficiency of 91% compared to native CalB and is expected to be very useful because it has high thermal and pH stability and excellent reusability.
Exosomal RNAs: Novel Potential Biomarkers for Diseases—A Review
Authors: Wang Jian , Yue Bing-Lin , Huang Yong-Zhen , Lan Xian-Yong , Liu Wu-Jun , Chen Hong
Published: February 2022

Exosomes are a subset of nano-sized extracellular vesicles originating from endosomes. Exosomes mediate cell-to-cell communication with their cargos, which includes mRNAs, miRNAs, lncRNAs, and circRNAs. Exosomal RNAs have cell specificity and reflect the conditions of their donor cells. Notably, their detection in biofluids can be used as a diagnostic marker for various diseases. Exosomal RNAs are ideal biomarkers because their surrounding membranes confer stability and they are detectable in almost all biofluids, which helps to reduce trauma and avoid invasive examinations. However, knowledge of exosomal biomarkers remains scarce. The present review summarizes the biogenesis, secretion, and uptake of exosomes, the current researches exploring exosomal mRNAs, miRNAs, lncRNAs, and circRNAs as potential biomarkers for the diagnosis of human diseases, as well as recent techniques of exosome isolation.
Effect of Irradiation on Structural Changes of Levan
Authors: Chelminiak-Dudkiewicz Dorota , Smolarkiewicz-Wyczachowski Aleksander , Wegrzynowska-Drzymalska Katarzyna , Ziegler-Borowska Marta
Published: February 2022

Levan, as a biocompatible and renewable biopolymer with anticancer properties, is a promising candidate for a wide range of applications in various fields of industry. However, in the literature, there is a lack of information about its behavior under the influence of UV irradiation, which may limit its potential application, including medical science. Therefore, this study describes the effects of irradiation on the structural properties of levan. This type of fructan was subjected to stability tests under radiation conditions using LED and polychromatic lamps. The results showed that the photodegradation of levan irradiated with a polychromatic light occurs faster and more efficiently than the photodegradation of levan irradiated with an LED lamp. Furthermore, AFM analysis showed that the surface became smoother after irradiation, as evidenced by decreasing values of roughness parameters. Moreover, UV irradiation causes the decrease of total surface free energy and both its components in levan; however, more significant changes occur during irradiation of the sample with a polychromatic lamp.
Genetic and Neurological Deficiencies in the Visual System of mct8 Mutant Zebrafish
Authors: Rozenblat Rotem , Tovin Adi , Zada David , Lebenthal-Loinger Ilana , Lerer-Goldshtein Tali , Appelbaum Lior
Published: February 2022

Thyroid hormones (THs; T3 and T4) enter cells using specific transporters and regulate development and metabolism. Mutation in the TH transporter monocarboxylate transporter 8 (MCT8, SLC16A2) is associated with brain hypothyroidism and neurological impairment. We established mct8 mutant (mct8−/−) zebrafish as a model for MCT8 deficiency, which causes endocrinological, neurological, and behavioral alterations. Here, we profiled the transcriptome of mct8−/− larvae. Among hundreds of differentially expressed genes, the expression of a cluster of vision-related genes was distinct. Specifically, the expression of the opsin 1 medium wave sensitive 2 (opn1mw2) decreased in two mct8 mutants: mct8−/− and mct8−25bp−/− larvae, and under pharmacological inhibition of TH production. Optokinetic reflex (OKR) assays showed a reduction in the number of conjugated eye movements, and live imaging of genetically encoded Ca2+ indicator revealed altered neuronal activity in the pretectum area of mct8−25bp−/− larvae. These results imply that MCT8 and THs regulate the development of the visual system and suggest a mechanism to the deficiencies observed in the visual system of MCT8-deficiency patients.
Dysregulation of Human Somatic piRNA Expression in Parkinson’s Disease Subtypes and Stages
Authors: Zhang Tianjiao , Wong Garry
Published: February 2022

Piwi interacting RNAs (piRNAs) are small non-coding single-stranded RNA species 20–31 nucleotides in size generated from distinct loci. In germline tissues, piRNAs are amplified via a “ping-pong cycle” to produce secondary piRNAs, which act in transposon silencing. In contrast, the role of somatic-derived piRNAs remains obscure. Here, we investigated the identity and distribution of piRNAs in human somatic tissues to determine their function and potential role in Parkinson’s disease (PD). Human datasets were curated from the Gene Expression Omnibus (GEO) database and a workflow was developed to identify piRNAs, which revealed 902 somatic piRNAs of which 527 were expressed in the brain. These were mainly derived from chromosomes 1, 11, and 19 compared to the germline tissues, which were from 15 and 19. Approximately 20% of somatic piRNAs mapped to transposon 3′ untranslated regions (UTRs), but a large proportion were sensed to the transcript in contrast to germline piRNAs. Gene set enrichment analysis suggested that somatic piRNAs function in neurodegenerative disease. piRNAs undergo dysregulation in different PD subtypes (PD and Parkinson’s disease dementia (PDD)) and stages (premotor and motor). piR-has-92056, piR-hsa-150797, piR-hsa-347751, piR-hsa-1909905, piR-hsa-2476630, and piR-hsa-2834636 from blood small extracellular vesicles were identified as novel biomarkers for PD diagnosis using a sparse partial least square discriminant analysis (sPLS-DA) (accuracy: 92%, AUC = 0.89). This study highlights a role for piRNAs in PD and provides tools for novel biomarker development.
Altered p16Ink4a, IL-1β, and Lamin b1 Protein Expression Suggest Cellular Senescence in Deep Endometriotic Lesions
Authors: Malvezzi Helena , Dobo Cristine , Filippi Renee Zon , Mendes do Nascimento Helen , Palmieri da Silva e Sousa Laura , Meola Juliana , Piccinato Carla Azevedo , Podgaec Sérgio
Published: February 2022

Endometriosis causes immunological and cellular alterations. Endometriosis lesions have lower levels of lamin b1 than the endometrium. Moreover, high levels of pro-inflammatory markers are observed in the peritoneal fluid, follicular fluid, and serum in endometriosis lesions. Thus, we hypothesized that the accumulation of senescent cells in endometriosis tissues would facilitate endometriosis maintenance in an inflammatory microenvironment. To study senescent cell markers and the senescence-associated secretory phenotype (SASP) in endometriosis lesions, we conducted a cross-sectional study with 27 patients undergoing video laparoscopy for endometriosis resection and 19 patients without endometriosis. Endometriosis lesions were collected from patients with endometriosis, while eutopic endometrium was collected from patients both with and without endometriosis. Tissues were evaluated for senescence markers (p16Ink4a, lamin b1, and IL-1β) and interleukin concentrations. The expression of p16Ink4a increased in lesions compared to that in eutopic endometrium from endometriosis patients in the secretory phase. In the proliferative phase, lesions exhibited lower lamin b1 expression but higher IL-4 expression than the eutopic endometrium. Further, IL-1β levels were higher in the lesions than in the eutopic endometrium in both the secretory and proliferative phases. We believe that our findings may provide targets for better therapeutic interventions to alleviate the symptoms of endometriosis.