Antioxidant Capacity of Myrciaria Cauliflora Seeds Extracts By Spectrophotometric, Biochemical and Electrochemical Methods Preferred Presentation
Authors: Mendes Hacke Ana Carolina , Valério Taynara , Cubo Mateus , Lima Dhésmon , Pessoa Christiana , Pereira Romaiana 
Published: July 2022

It is well known that fruit processing industries lead to the generation of large amounts of by-products every year, such as seeds and peels, which are frequently considered as waste. However, it has already been proved that these by-products can consist in a rich source of bioactive compounds with valuable nutritional and biological effects (1). In this perspective, there is a growing interest in the discovery of natural antioxidants which can be used as an alternative to synthetic substances by pharmaceutical and food industries (2). Jabuticaba (Myrciaria cauliflora) is a typical fruit from Brazil that belongs to Myrtaceae family. Its seeds are usually discarded as waste by industries and studies have already proven that these by-products are rich in bioactive compounds with promising biological effects (3,4). In this study, it was evaluated the antioxidant activity of hydroalcoholic (EtOH), methanolic (MEtOH), aqueous (AQ) and propanone (AQAc) extracts from jabuticaba seeds. This effect was assessed by their ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2’-azino-bis (ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), hypochlorous acid (HOCl), and superoxide anion (O2
•−) radicals. The redox properties of jabuticaba seed extracts were studied by using differential pulse voltammetry (DPV). Their protective effect to prevent in vitro oxidative cellular damages induced by 2,2-azobis(2-aminopropanone) dihydrochloride (AAPH) in human erythrocytes was also investigated. The obtained results revealed that all extracts were able to scavenge free radicals used in this work, due to their lower IC50 (inhibitory concentration) values. A similarity between E
pa values suggested that the extracts were constituted by analogous electroactive species and exhibited similar antioxidant effect in this assay. However, it was observed that the AQAc extract presented the lowest E
pa value (115.1 ± 0.95 mV) indicating a higher antioxidant activity when compared to the other extracts (p < 0.05). Furthermore, it was showed a good positive correlation between the results obtained by using DPV and the synthetic and biological radicals (DPPH•: r = 0.8215; ABTS•+: r = 0.9123; HOCl: r = 0.8193, and O2
•-: r = 0.9916). The extracts protected human erythrocytes against oxidative cellular damage caused by AAPH, which was confirmed by using field-emission gun scanning electron microscopy (FESEM) analysis. Taken together, the results reported in this work evidence that jabuticaba seeds are a source of antioxidant compounds that present a remarkable potential to prevent damage related to oxidative stress in biological systems. In this way, our work suggests the use of this fruit byproduct in further biochemical studies aiming its use as a new nutraceutical and food additive.

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