bluuebunny/crossref_metadata_2025 huggingface dataset:
GJB2 and GJB6 Mutations in Hereditary Recessive Non-Syndromic Hearing Impairment in Cameroon
Authors: Tingang Wonkam Edmond , Chimusa Emile , Noubiap Jean Jacques , Adadey Samuel Mawuli , F. Fokouo Jean Valentin , Wonkam Ambroise
Published: October 2019

This study aimed to investigate GJB2 (connexin 26) and GJB6 (connexin 30) mutations associated with familial non-syndromic childhood hearing impairment (HI) in Cameroon. We selected only families segregating HI, with at least two affected individuals and with strong evidence of non-environmental causes. DNA was extracted from peripheral blood, and the entire coding region of GJB2 was interrogated using Sanger sequencing. Multiplex PCR and Sanger sequencing were used to analyze the prevalence of the GJB6-D3S1830 deletion. A total of 93 patients, belonging to 41 families, were included in the analysis. Hearing impairment was sensorineural in 51 out of 54 (94.4%) patients. Pedigree analysis suggested autosomal recessive inheritance in 85.4% (35/41) of families. Hearing impairment was inherited in an autosomal dominant and mitochondrial mode in 12.2% (5/41) and 2.4% (1/41) of families, respectively. Most HI participants were non-syndromic (92.5%; 86/93). Four patients from two families presented with type 2 Waardenburg syndrome, and three cases of type 2 Usher syndrome were identified in one family. No GJB2 mutations were found in any of the 29 families with non-syndromic HI. Additionally, the GJB6-D3S1830 deletion was not identified in any of the HI patients. This study confirms that mutations in the GJB2 gene and the del(GJB6-D13S1830) mutation do not contribute to familial HI in Cameroon.
“Zwischen allen Stühlen”: Reflections on Judaism in Germany in Victor Klemperer’s Post-Holocaust Diaries
Authors: Sepp Arvi
Published: October 2019

This article focuses as a case study on Victor Klemperer’s diaristic representation of German-Jewish identity and culture after 1945 in the Soviet Occupation Zone and the GDR. The contribution shows how Klemperer’s professional and social situation remained very uncomfortable even in East Germany. For the diarist, the communist code ‘antifascist/fascist’, just like the code ‘German/un-German’ before it, was tantamount to concealing Jewish origin. His post-Holocaust journals provide an immediate insider’s view of Jewish life in Germany after the Holocaust from the perspective of a victim of active persecution. Against this backdrop, the contribution examines how the author’s original German nationalism gradually makes way, caught between contradictory impulses of assimilation and decreed Jewish identity, for a much more complex understanding of his own cultural identity. Klemperer’s diaries highlight a number of tensions that ultimately reflect on the disjunction between living and writing: The divide between a single and changing self lies at the heart of his diaries after 1945, which depict an astute, complex psychogram of the assimilated German-Jewish bourgeoisie that survived the Holocaust and tried to continue living in communist Germany.
Variations in Parent and Teacher Ratings of Internalizing, Externalizing, Adaptive Skills, and Behavioral Symptoms in Children with Selective Mutism
Authors: Klein Evelyn R. , Ruiz Cesar E. , Morales Kylee , Stanley Paige
Published: October 2019

Selective mutism (SM) is an anxiety disorder that impacts communication. Children with SM present concerns to parents and teachers as they consistently do not speak in situations where there is an expectation to speak, such as at school, but speak in other settings where they feel more comfortable, such as at home. The purpose of this study was to investigate the relationship between parents’ and teachers’ perceptions of children with SM on behavioral rating scales and language measures. Forty-two children (22 boys and 20 girls, ranging from 2.4 to 13.8 years, with a mean age of 7.1 years) took part in this study. Parents and teachers completed the Behavior Assessment System for Children (BASC-3) measuring internalizing behaviors, externalizing behaviors, adaptive skills, and behavioral symptoms. Frequency of speaking and language abilities were also measured. Parents and teachers both identified withdrawal as the most prominent feature of SM but parents saw children as significantly more withdrawn than did their teachers. Both rated children similarly at-risk on scales of functional communication and social skills. Higher adaptive skills (including functional communication and social skills) were positively correlated with vocabulary, narrative language, and auditory serial memory according to teachers. Parent and teacher rating scales provide valuable information for diagnosis and progress monitoring. Children with SM can benefit from mental health practitioners who can identify and enhance their emotional well-being.
Hierarchical Semantic Correspondence Analysis on Feature Classes between Two Geospatial Datasets Using a Graph Embedding Method
Authors: Huh Yong
Published: October 2019

A method to find corresponding feature class pairs, including hierarchical M:N pairs between two geospatial datasets is proposed. Applying an overlapping analysis to the object sets within the feature classes, the similarities of the feature classes are estimated and projected onto a lower-dimensional vector space after applying the graph embedding method. In this space, conventional mathematical tools—agglomerative hierarchical clustering in this study—could be used to analyze semantic correspondences between the datasets and identify their hierarchical M:N corresponding pairs. The proposed method was applied to two cadastral parcel datasets; one for latest land-use records in an urban information system, and the other, for original land-use categories in the Korea land information system. To quantitatively assess identified feature pairs, F-measures for each pair are presented. The results showed that it was possible to find various semantic correspondences of the feature classes and infer regional land development characteristics.
The Role of the Gut Microbiota in Colorectal Cancer Causation
Authors: Alhinai Eiman A. , Walton Gemma E. , Commane Daniel M.
Published: October 2019

Here, we reviewed emerging evidence on the role of the microbial community in colorectal carcinogenesis. A healthy gut microbiota promotes intestinal homeostasis and can exert anti-cancer effects; however, this microbiota also produces a variety of metabolites that are genotoxic and which can negatively influence epithelial cell behaviour. Disturbances in the normal microbial balance, known as dysbiosis, are frequently observed in colorectal cancer (CRC) patients. Microbial species linked to CRC include certain strains of Bacteroides fragilis, Escherichia coli, Streptococcus gallolyticus, Enterococcus faecalis and Fusobacterium nucleatum, amongst others. Whether these microbes are merely passive dwellers exploiting the tumour environment, or rather, active protagonists in the carcinogenic process is the subject of much research. The incidence of chemically-induced tumours in mice models varies, depending upon the presence or absence of these microorganisms, thus strongly suggesting influences on disease causation. Putative mechanistic explanations differentially link these strains to DNA damage, inflammation, aberrant cell behaviour and immune suppression. In the future, modulating the composition and metabolic activity of this microbial community may have a role in prevention and therapy.
Wideband Spectrum Sensing Method Based on Channels Clustering and Hidden Markov Model Prediction
Authors: Wang Huan , Wu Bin , Yao Yuancheng , Qin Mingwei
Published: October 2019

Spectrum sensing is the necessary premise for implementing cognitive radio technology. The conventional wideband spectrum sensing methods mainly work with sweeping frequency and still face major challenges in performance and efficiency. This paper introduces a new wideband spectrum sensing method based on channels clustering and prediction. This method counts on the division of the wideband spectrum into uniform sub-channels, and employs a density-based clustering algorithm called Ordering Points to Identify Clustering Structure (OPTICS) to cluster the channels in view of the correlation between the channels. The detection channel (DC) is selected and detected for each cluster, and states of other channels (estimated channels, ECs) in the cluster are then predicted with Hidden Markov Model (HMM), so that all channels states of the wideband spectrum are finally obtained. The simulation results show that the proposed method could effectively improve the wideband spectrum sensing performance.
Advances and Challenges of Using the Sterile Insect Technique for the Management of Pest Lepidoptera
Authors: Marec František , Vreysen Marc J. B.
Published: October 2019

Over the past 30 years, the sterile insect technique (SIT) has become a regular component of area-wide integrated pest management (AW-IPM) programs against several major agricultural pests and vectors of severe diseases. The SIT-based programs have been especially successful against dipteran pests. However, the SIT applicability for controlling lepidopteran pests has been challenging, mainly due to their high resistance to the ionizing radiation that is used to induce sterility. Nevertheless, the results of extensive research and currently operating SIT programs show that most problems with the implementation of SIT against pest Lepidoptera have been successfully resolved. Here, we summarize the cytogenetic peculiarities of Lepidoptera that should be considered in the development and application of SIT for a particular pest species. We also discuss the high resistance of Lepidoptera to ionizing radiation, and present the principle of derived technology based on inherited sterility (IS). Furthermore, we present successful SIT/IS applications against five major lepidopteran pests, and summarize the results of research on the quality control of reared and released insects, which is of great importance for their field performance. In the light of new research findings, we also discuss options for the development of genetic sexing strains, which is a challenge to further improve the applicability of SIT/IS against selected lepidopteran pests.
Biomechanical Effects of a New Macrogeometry Design of Dental Implants: An In Vitro Experimental Analysis
Authors: Gehrke Sergio Alexandre , Pérez-Díaz Leticia , Mazón Patricia , De Aza Piedad N.
Published: October 2019

The purpose of the present study was to measure and compare the insertion torque, removal torque, and the implant stability quotient by resonance frequency analysis in different polyurethane block densities of two implant macrogeometries. Four different polyurethane synthetic bone blocks were used with three cortical thickness: Bone 1 with a cortical thickness of 1 mm, Bone 2 with a cortical thickness of 2 mm, Bone 3 with a cortical thickness of 3 mm, and Bone 4, which was totally cortical. Four groups were created in accordance with the implant macrogeometry (n = 10 per group) and surface treatment: G1—regular implant design without surface treatment; G2—regular implant design with surface treatment; G3—new implant design without surface treatment; G4—new implant design with surface treatment. All implants used were 4 mm in diameter and 10 mm in length and manufactured in commercially pure titanium (grade IV) by Implacil De Bortoli (São Paulo, Brazil). The implants were installed using a computed torque machine, and following installation of the implant, the stability quotient (implant stability quotient, ISQ) values were measured in two directions using Osstell devices. The data were analyzed by considering the 5% level of significance. All implant groups showed similar mean ISQ values without statistical differences (p > 0.05), for the same synthetic bone block: for Bone 1, the value was 57.7 ± 3.0; for Bone 2, it was 58.6 ± 2.2; for Bone 3, it was 60.6 ± 2.3; and for Bone 4, it was 68.5 ± 2.8. However, the insertion torque showed similar higher values for the regular macrogeometry (G1 and G2 groups) in comparison with the new implant macrogeometry (G3 and G4 groups). The analysis of the results found that primary stability does not simply depend on the insertion torque but also on the bone quality. In comparison with the regular implant macrogeometry, the new implant macrogeometry decreased the insertion torque without affecting the implant stability quotient values.
Investigation on Fatigue Threshold Testing Methods in a Near Lamellar TiAl Alloy
Authors: Wang Shiyuan , Li Hangyue , Bowen Paul
Published: October 2019

The effects of influential fatigue testing factors, including loading schemes (e.g., traditional load shedding and staircase load increasing), morphology of crack starters, and precracking approaches on the near-threshold fatigue crack growth behaviors for a near lamellar γ-TiAl alloy (Ti-45Al-2Mn-2Nb-1B) were investigated at room temperature and 650 °C. The results showed that the measured fatigue threshold values in lamellar γ-TiAl alloys are very sensitive to the applied testing procedures. For example, the staircase load-increasing method yielded smaller threshold values. When such a load-increasing method was used, the threshold values were measured either from a notch machined by electro-discharge machining or prepared by a compression–compression fatigue loading. Moreover, some differences could be seen with respect to the morphologies of the crack starters. Most of the above influences are associated with the brittle nature of the material and the characteristics of the lamellar microstructures, and closure effects are primarily induced by crack wake roughness or unbroken ligaments.
On Expansive Mappings
Authors: Markin Marat V. , Sichel Edward S.
Published: October 2019

When finding an original proof to a known result describing expansive mappings on compact metric spaces as surjective isometries, we reveal that relaxing the condition of compactness to total boundedness preserves the isometry property and nearly that of surjectivity. While a counterexample is found showing that the converse to the above descriptions do not hold, we are able to characterize boundedness in terms of specific expansions we call anticontractions.
Parametric Analysis of Macro-Geometrical Deviations in Dry Turning of UNS A97075 (Al-Zn) Alloy
Authors: Martín Béjar Sergio , Trujillo Vilches Francisco Javier , Bermudo Gamboa Carolina , Sevilla Hurtado Lorenzo
Published: October 2019

Macro-geometrical deviations play a very important role in the functionality and reliability of structural parts for aircraft. The use of environmentally friendly techniques, such as dry machining, may negatively affect these deviations. Despite its importance, there is a lack of research that analyzes them as a function of the cutting parameters in the case of aluminum alloys for aeronautical purpose. In this work, the cutting speed and feed influence on several macro-geometrical deviations (parallelism, straightness, circular run-out, roundness, concentricity, total circular run-out and cylindricity) in dry turning of UNS A97075 alloy was analyzed. The main novelty of this work lies in the use of high slenderness parts used in further fatigue tests. The results showed that feed seems to be the most influential parameter in most of the deviations studied. In addition, the parts with lower rigidity exhibited higher sensitivity to change with the cutting parameters. Finally, different parametric models were proposed to obtain the geometrical deviations as a function of the cutting parameters.
Evaluation of Heat Sources for the Simulation of the Temperature Distribution in Gas Metal Arc Welded Joints
Authors: Chiocca Andrea , Frendo Francesco , Bertini Leonardo
Published: October 2019

Residual stresses can affect both the static strength and the fatigue endurance of welded joints. Residual stresses can be assessed by numerical simulation; however, the simulation of the welding process is a complex task that requires knowledge of several parameters, many of which can only be estimated with some uncertainty. The reduction in the number of these parameters can lead to a more feasible and efficient study. In this work, the finite element method is used to assess the capability of different thermal methods used to simulate a single pass of the gas metal arc welding process in reproducing the temperature distribution around the weld. Results of the simulations are compared to experimental measurements of the surface temperature close to the welding area. The thermal techniques analyzed adopt different levels of complexity, from the basic implementation of a constant initial temperature assigned to a given material volume, to the more comprehensive and widespread Goldak’s double-ellipsoid model. The study shows that, close to the weld seam, very similar thermal behaviors can be achieved by employing each one of the analyzed methods. Secondly, considering the constant initial temperature method, the comparison between experimental measurements and numerical simulations showed a fairly good agreement, suggesting that a relatively simple method (i.e., requiring the setting of only one parameter) can be used to efficiently reproduce the thermal history of a welding process.
Interface Microstructure and Nanoindentation Characterization of Laser Offset Welded 5052 Aluminum to Press-Hardened Steel Using a Brass Interlayer
Authors: Cao Xiaobing , Zhou Xiongfeng , Li Zhou , Luo Zhi , Duan Ji’an
Published: October 2019

Laser offset welding of 5052 aluminum to press-hardened steel using a brass interlayer was carried out. The cross-sectioned macrostructure and tensile strength were governed by varying the thickness of the brass interlayer. The maximum tensile strength reached 56.4 MPa when the thickness of brass interlayer was 0.05 mm. Subsequently, the interface microstructure, the nanoindentation characterization, and the fracture behavior were evaluated experimentally by means of scanning electron microscopy (SEM), energy-dispersive X-ray spectrometer (EDS), and micro-X-ray diffraction (micro-XRD), respectively. It was found that the intermetallic compound (IMC) layer at the interface consisted of an Fe2Al5 layer and an FeAl layer, and the estimated nanohardness of Fe2Al5, FeAl, and Fe3Al were 16.11 GPa, 9.48 GPa, and 4.13 GPa, respectively. The fracture of the joint with the 0.05 mm brass interlayer was a mixture of cleavage fracture and intergranular fracture, while that of the joint with the 0.1 mm brass interlayer exhibited the characterization of a major dendrite arm, leaving a metallurgical connected zone consisting of the Al2Cu and the α-Al phase.
Influence of Thermal and Magnetic History on Direct ΔTad Measurements of Ni49+xMn36−xIn15 Heusler Alloys
Authors: Moreno-Ramírez Luis M. , Delgado-Matarín Antonio , Law Jia Yan , FRANCO Victorino , Conde Alejandro , Giri Anit K.
Published: October 2019

In the present work, using Heusler Ni49+xMn36-xIn15 (with x = 0 and 0.5) alloys, it is shown that the choice of the appropriate measurement protocol (erasing the prior state of the sample in between experiments) in ∆Tad first shot characterization is crucial for obtaining reliable results. Unlike indirect measurements, for which incorrect protocols produce overestimates of the characteristics of the material, erroneous direct measurements underestimate ∆Tad in the region close to its first order phase transition. The error in ∆Tad is found to be dependent on the temperature step used, being up to ~40% underestimation, including a slight shift in its peak temperature.
Quantitative 1H NMR Metabolomics Reveal Distinct Metabolic Adaptations in Human Macrophages Following Differential Activation
Authors: Fuchs Amanda L. , Schiller Sage M. , Keegan Wyatt J. , Ammons Mary Cloud B. , Eilers Brian , Tripet Brian , Copié Valérie
Published: October 2019

Macrophages (MΦs) are phagocytic immune cells that are found in nearly all human tissues, where they modulate innate and adaptive immune responses, thereby maintaining cellular homeostasis. MΦs display a spectrum of functional phenotypes as a result of microenvironmental and stress-induced stimuli. Evidence has emerged demonstrating that metabolism is not only crucial for the generation of energy and biomolecular precursors, but also contributes to the function and plasticity of MΦs. Here, 1D 1H NMR-based metabolomics was employed to identify metabolic pathways that are differentially modulated following primary human monocyte-derived MΦ activation with pro-inflammatory (M1) or anti-inflammatory (M2a) stimuli relative to resting (M0) MΦs. The metabolic profiling of M1 MΦs indicated a substantial increase in oxidative stress as well as a decrease in mitochondrial respiration. These metabolic profiles also provide compelling evidence that M1 MΦs divert metabolites from de novo glycerophospholipid synthesis to inhibit oxidative phosphorylation. Furthermore, glycolysis and lactic acid fermentation were significantly increased in both M1 and M2a MΦs. These metabolic patterns highlight robust metabolic activation markers of MΦ phenotypes. Overall, our study generates additional support to previous observations, presents novel findings regarding the metabolic modulation of human MΦs following activation, and contributes new knowledge to the rapidly evolving field of immunometabolism.
Abscisic Acid Receptors Modulate Metabolite Levels and Phenotype in Arabidopsis Under Normal Growing Conditions
Authors: Li Xiaoyi , Wu Lintao , Qiu Yao , Wang Tao , Zhou Qin , Zhang Qian , Zhang Wei , Liu Zhibin
Published: October 2019

Abscisic acid (ABA) is a vital phytohormone that accumulates in response to various biotic and abiotic stresses, as well as plant growth. In Arabidopsis thaliana, there are 14 members of the ABA receptor family, which are key positive regulators involved in ABA signaling. Besides reduced drought stress tolerance, the quadruple and sextuple mutants (pyr1pyl1pyl2pyl4 (1124) and pyr1pyl1pyl2pyl4pyl5pyl8 (112458) show abnormal growth phenotypes, such as decreases in yield and height, under non-stress conditions. However, it remains unknown whether ABA receptors mediate ABA signaling to regulate plant growth and development. Here, we showed the primary metabolite profiles of 1124, 112458 and wild-type (WT) plants grown under normal conditions. The metabolic changes were significantly different between ABA receptor mutants and WT. Guanosine, for the biosynthesis of cyclic guanosine 3′,5′-monophosphate (cGMP), is an important second messenger that acts to regulate the level of ABA. In addition, other amino acids were increased in the 112458 mutant, including proline. These results, together with phenotype analysis, indicated that ABA receptors are involved in ABA signaling to modulate metabolism and plant growth under normal conditions.
Analytic Correlation Filtration: A New Tool to Reduce Analytical Complexity of Metabolomic Datasets
Authors: Monnerie Stephanie , Petera Melanie , Lyan Bernard , Gaudreau Pierrette , Comte Blandine , Pujos-Guillot Estelle
Published: October 2019

Metabolomics generates massive and complex data. Redundant different analytical species and the high degree of correlation in datasets is a constraint for the use of data mining/statistical methods and interpretation. In this context, we developed a new tool to detect analytical correlation into datasets without confounding them with biological correlations. Based on several parameters, such as a similarity measure, retention time, and mass information from known isotopes, adducts, or fragments, the algorithm principle is used to group features coming from the same analyte, and to propose one single representative per group. To illustrate the functionalities and added-value of this tool, it was applied to published datasets and compared to one of the most commonly used free packages proposing a grouping method for metabolomics data: ‘CAMERA’. This tool was developed to be included in Galaxy and is available in Workflow4Metabolomics.
Gas Pressure Sensor Based on BDK-Doped Polymer Optical Fiber
Authors: Cheng Xin , Liu Yi , Yu Changyuan
Published: October 2019

This paper presents a high sensitivity gas pressure sensor with benzyl-dimethylketal (BDK)-doped polymer optical fiber Bragg grating (POFBG), whose sensitivity is up to 8.12 pm/kPa and 12.12 pm/kPa in positive and negative pressure atmosphere, respectively. The high sensitivity can be explained by its porous chemical structure. The stability and response behavior under air pressure atmosphere has also been investigated. The new understanding of the air pressure response principle and sensitivity difference for the presented sensor can be a worthy reference.
Fluoride-Ion Batteries: On the Electrochemical Stability of Nanocrystalline La0.9Ba0.1F2.9 against Metal Electrodes
Authors: Gombotz Maria , Pregartner Veronika , Hanzu Ilie , Wilkening H. Martin R.
Published: October 2019

Over the past years, ceramic fluorine ion conductors with high ionic conductivity have stepped into the limelight of materials research, as they may act as solid-state electrolytes in fluorine-ion batteries (FIBs). A factor of utmost importance, which has been left aside so far, is the electrochemical stability of these conductors with respect to both the voltage window and the active materials used. The compatibility with different current collector materials is important as well. In the course of this study, tysonite-type La 0.9 Ba 0.1 F 2.9 , which is one of the most important electrolyte in first-generation FIBs, was chosen as model substance to study its electrochemical stability against a series of metal electrodes viz. Pt, Au, Ni, Cu and Ag. To test anodic or cathodic degradation processes we carried out cyclic voltammetry (CV) measurements using a two-electrode set-up. We covered a voltage window ranging from −1 to 4 V, which is typical for FIBs, and investigated the change of the response of the CVs as a function of scan rate (2 mV/s to 0.1 V/s). It turned out that Cu is unstable in combination with La 0.9 Ba 0.1 F 2.9 , even before voltage was applied. The cells with Au and Pt electrodes show reactions during the CV scans; in the case of Au the irreversible changes seen in CV are accompanied by a change in color of the electrode as investigated by light microscopy. Ag and Ni electrodes seem to suffer from contact issues which, most likely, also originate from side reactions with the electrode material. The experiments show that the choice of current collectors in future FIBs will become an important topic if we are to develop long-lasting FIBs. Most likely, protecting layers between the composite electrode material and the metal current collector have to be developed to prevent any interdiffusion or electrochemical degradation processes.
Enhancing Photovoltaic Performance of GaAs Single-Junction Solar Cells by Applying a Spectral Conversion Layer Containing Eu-Doped and Yb/Er-Doped Phosphors
Authors: Ho Wen-Jeng , Liu Jheng-Jie , Lin Zong-Xian , Shiao Hung-Pin
Published: October 2019

In this study, we examined efforts to increase the photovoltaic performance of GaAs single-junction solar cells using spectral conversion layers, respectively, composed of europium-doped (Eu-doped) phosphors, ytterbium/erbium-doped (Yb/Er-doped) phosphors, and a combination of Eu-doped and Yb/Er-doped phosphors. Spin-on film deposition was used to apply the conversion layers, all of which had a total phosphor concentration of 3 wt%. The chemical compositions of the phosphors were examined by energy-dispersive X-ray spectroscopy. The fluorescence emissions of the phosphors were confirmed by using photoluminescence measurements. Under laser diode excitation at 405 nm, we observed green luminescent downshift (LDS) emissions by Eu-doped phosphors at wavelengths of 479 nm to 557 nm, and under excitation at 980 nm, we observed red up-conversion (UC) emissions by Yb/Er-doped phosphors at wavelengths of 647 nm to 672 nm. The spectral conversion layers were characterized in terms of optical reflectance, external quantum efficiency, and photovoltaic current and voltage under AM 1.5 G simulations. The conversion efficiency of the cell combining Eu-doped and Yb/Er-doped phosphors (23.84%) exceeded that of the cell coated with Yb/Er-doped phosphors (23.72%), the cell coated with Eu-doped phosphors (23.19%), and the cell coated without phosphors (22.91%).
Docosahexaenoic Acid Inhibits PTP1B Phosphatase and the Viability of MCF-7 Breast Cancer Cells
Authors: Kuban-Jankowska Alicja , Gorska-Ponikowska Magdalena , Sahu Kamlesh Kumar , Kostrzewa Tomasz , Wozniak Michal , Tuszynski Jack
Published: October 2019

Background: Docosahexaenoic acid (DHA) is an essential polyunsaturated fatty acid compound present in deep water fishes and dietary supplements, with a wide spectrum of potential health benefits, ranging from neurological to anti-inflammatory. Methods: Due to the fact that DHA is considered a breast cancer risk reducer, we examined the impact of DHA on MCF-7 breast cancer cells’ viability and its inhibitory properties on protein tyrosine phosphatase 1B (PTP1B), a pro-oncogenic phosphatase. Results: We found that DHA is able to lower both the enzymatic activity of PTP1B phosphatase and the viability of MCF-7 breast cancer cells. We showed that unsaturated DHA possesses a significantly higher inhibitory activity toward PTP1B in comparison to similar fatty acids. We also performed a computational analysis of DHA binding to PTP1B and discovered that it is able to bind to an allosteric binding site. Conclusions: Utilizing both a recombinant enzyme and cellular models, we demonstrated that DHA can be considered a potential pharmacological agent for the prevention of breast cancer.
Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
Authors: Takahashi Yumiko , Matsunaga Yutaka , Banjo Mai , Takahashi Kenya , Sato Yosuke , Seike Kohei , Nakano Suguru , Hatta Hideo
Published: October 2019

We investigated the effects of nutrient intake timing on glycogen accumulation and its related signals in skeletal muscle after an exercise that did not induce large glycogen depletion. Male ICR mice ran on a treadmill at 25 m/min for 60 min under a fed condition. Mice were orally administered a solution containing 1.2 mg/g carbohydrate and 0.4 mg/g protein or water either immediately (early nutrient, EN) or 180 min (late nutrient, LN) after the exercise. Tissues were harvested at 30 min after the oral administration. No significant difference in blood glucose or plasma insulin concentrations was found between the EN and LN groups. The plantaris muscle glycogen concentration was significantly (p < 0.05) higher in the EN group—but not in the LN group—compared to the respective time-matched control group. Akt Ser473 phosphorylation was significantly higher in the EN group than in the time-matched control group (p < 0.01), while LN had no effect. Positive main effects of time were found for the phosphorylations in Akt substrate of 160 kDa (AS160) Thr642 (p < 0.05), 5′-AMP-activated protein kinase (AMPK) Thr172 (p < 0.01), and acetyl-CoA carboxylase Ser79 (p < 0.01); however, no effect of nutrient intake was found for these. We showed that delayed nutrient intake could not increase muscle glycogen after endurance exercise which did not induce large glycogen depletion. The results also suggest that post-exercise muscle glycogen accumulation after nutrient intake might be partly influenced by Akt activation. Meanwhile, increased AS160 and AMPK activation by post-exercise fasting might not lead to glycogen accumulation.
Relationship between 1,25-Dihydroxyvitamin D and Body Composition in Middle-Aged Sedentary Adults: The FIT-AGEING Study
Authors: De-la-O Alejandro , Jurado-Fasoli Lucas , Castillo Manuel J. , Gracia-Marco Luis , Gutierrez Ángel , Amaro-Gahete Francisco J.
Published: October 2019

Vitamin D deficiency is a worldwide health problem that, in addition to its well-known negative effects on musculoskeletal health, has been related to a wide range of acute and chronic age-related diseases. However, little is known about the association of body composition with the active, hormonal form of vitamin D, 1,25-dihydroxyvitamin D plasma levels (1,25(OH)2D). Therefore, the aim of this study was to investigate the association of 1,25(OH)2D with body composition including lean and fat body mass as well as bone mineral density (BMD) in middle-aged sedentary adults. A total of 73 (39 women) middle-aged sedentary adults (53.7 ± 5.1 years old) participated in the current study. We measured weight and height, and we used dual energy X-ray absorptiometry to measure lean body mass, fat body mass and BMD. Body mass index (BMI), lean mass index (LMI), and fat mass index (FMI) were calculated. 1,25(OH)2D was measured using a DiaSorin Liaison® immunochemiluminometric analyzer. The results showed a negative association of 1,25(OH)2D with BMI, LMI and BMD (β = −0.274, R2 = 0.075, p = 0.019; β = −0.268, R2 = 0.072, p = 0.022; and β = −0.325, R2 = 0.105, p = 0.005, respectively), which persisted after controlling for age and sex. No significant differences in 1,25(OH)2D across body weight status were observed after controlling for the same covariates. In summary, our results suggest that 1,25(OH)2D could be negatively associated with BMI, LMI and BMD whereas no association was found with FMI in middle-aged sedentary adults.
Fatty Acid Profile and Antioxidant Status Fingerprint in Sarcopenic Elderly Patients: Role of Diet and Exercise
Authors: Corsetto Paola Antonia , Montorfano Gigliola , Klersy Catherine , Massimino Luca , Infantino Vittoria , Iannello Giancarlo , Anna Faliva Milena , Lukaski Henry , Perna Simone , Alalwan Tariq A. , Rizzo Angela Maria , Rondanelli Mariangela
Published: October 2019

Plasma fatty acids (FAs) and oxidant status contribute to the etiology of sarcopenia in the elderly concurring to age-related muscle loss and elderly frailty through several mechanisms including changes in FA composition within the sarcolemma, promotion of chronic low-grade inflammation, and insulin resistance. The aim of this study was to determine the FA profile and pro-antioxidant status in sarcopenic frail elderly patients enrolled in a nutritional and physical activity program and to evaluate their correlation with clinical markers. Moreover, the possible changes, produced after a short-term clinical protocol, were evaluated. Plasma and erythrocyte FA composition and pro-antioxidant status were analyzed in sarcopenic elderly subjects recruited for the randomized clinical study and treated with a placebo or dietary supplement, a personalized diet, and standardized physical activity. Subjects were tested before and after 30 days of treatment. Pearson correlations between biochemical parameters and patients’ characteristics at recruitment indicate interesting features of sarcopenic status such as negative correlation among the plasma FA profile, age, and physical characteristics. Physical activity and dietetic program alone for 30 days induced a decrease of saturated FA concentration with a significant increase of dihomo-gamma-linolenic acid. Supplementation plus physical activity induced a significant decrease of linoleic acid, omega-6 polyunsaturated FAs, and an increase of stearic and oleic acid concentration. Moreover, glutathione reductase activity, which is an indicator of antioxidant status, significantly increased in erythrocytes. Changes over time between groups indicate significant differences for saturated FAs, which suggest that the amino acid supplementation restores FA levels that are consumed during physical activity. A relationship between FA and clinical/metabolic status revealed unique correlations and a specific metabolic and lipidomic fingerprint in sarcopenic elderly. The results indicate the positive beneficial role of supplementation and physical activity on plasma FA status and the antioxidant system as a co-adjuvant approach in sarcopenic, frail, elderly patients.
Role of Citrate in Pathophysiology and Medical Management of Bone Diseases
Authors: Granchi Donatella , Baldini Nicola , Ulivieri Fabio Massimo , Caudarella Renata
Published: October 2019

Citrate is an intermediate in the “Tricarboxylic Acid Cycle” and is used by all aerobic organisms to produce usable chemical energy. It is a derivative of citric acid, a weak organic acid which can be introduced with diet since it naturally exists in a variety of fruits and vegetables, and can be consumed as a dietary supplement. The close association between this compound and bone was pointed out for the first time by Dickens in 1941, who showed that approximately 90% of the citrate bulk of the human body resides in mineralised tissues. Since then, the number of published articles has increased exponentially, and considerable progress in understanding how citrate is involved in bone metabolism has been made. This review summarises current knowledge regarding the role of citrate in the pathophysiology and medical management of bone disorders.
Altilix® Supplement Containing Chlorogenic Acid and Luteolin Improved Hepatic and Cardiometabolic Parameters in Subjects with Metabolic Syndrome: A 6 Month Randomized, Double-Blind, Placebo-Controlled Study
Authors: Castellino Giuseppa , Nikolic Dragana , Magán-Fernández Antonio , Malfa Giuseppe Antonio , Chianetta Roberta , Patti Angelo M. , Amato Antonella , Montalto Giuseppe , Toth Peter P. , Banach Maciej , Cicero Arrigo F. G. , Rizzo Manfredi
Published: October 2019

The objective was to evaluate the effects of 6 months of supplementation with Altilix®, containing chlorogenic acid and its derivatives, and luteolin and its derivatives, on cardiovascular risk and hepatic markers in subjects with metabolic syndrome (MetS). A randomized, double-blind, placebo-controlled study was performed in 100 subjects with MetS with a follow-up period of 6 months; 50 subjects were randomized to Altilix® (26 men and 24 women, mean age 63 ± 8 years) and the other 50 to placebo (28 men and 22 women, mean age 63 ± 11 years). Anthropometric, cardiometabolic, and hepatic parameters were assessed at baseline and at the end of follow-up. Carotid intima-media thickness and endothelial function were assessed by doppler ultrasound and by flow-mediated dilation of the brachial artery, respectively. The presence and degree of non-alcoholic fatty liver disease (NAFLD) was assessed by the fatty liver index (FLI), and subjects were divided into three subgroups: (1) without NAFLD; (2) with borderline NAFLD; and (3) with NAFLD. After 6 months of Altilix® supplementation, we found a significant improvement vs. placebo in most of the evaluated parameters, including body weight (−2.40% (95% CI −3.79, −1.01); p < 0.001), waist circumference (−2.76% (95% CI −4.55, −0.96); p = 0.003), HbA1c (−0.95% (95% CI −1.22, −0.67); p < 0.001), plasma lipids, FLI (−21.83% (95% CI −27.39, −16.27); p < 0.001), hepatic transaminases, flow-mediated dilation (10.56% (95% CI 5.00, 16.12); p < 0.001), and carotid intima-media thickness (−39.48% (95% CI −47.98, −30.97); p < 0.001). Further, the improvement in cardiometabolic variables was independent of the degree of hepatic steatosis. Altilix® supplementation improved hepatic and cardio-metabolic parameters in MetS subjects. Altilix® supplementation was a beneficial approach in the management of hepatic and cardiometabolic alterations in MetS subjects.
Resorbable Beads Provide Extended Release of Antifungal Medication: In Vitro and In Vivo Analyses
Authors: Hsu Yung-Heng , Chen Huang-Yu , Chen Jin-Chung , Yu Yi-Hsun , Chou Ying-Chao , Ueng Steve Wen-Neng , Liu Shih-Jung
Published: October 2019

Fungal osteomyelitis has been difficult to treat, with first-line treatments consisting of implant excision, radical debridement, and local release of high-dose antifungal agents. Locally impregnated antifungal beads are another popular treatment option. This study aimed to develop biodegradable antifungal-agent-loaded Poly(d,l-lactide-co-glycolide) (PLGA) beads and evaluate the in vitro/in vivo release patterns of amphotericin B and fluconazole from the beads. Beads of different sizes were formed using a compression-molding method, and their morphology was evaluated via scanning electron microscopy. Intrabead incorporation of antifungal agents was evaluated via Fourier-transform infrared spectroscopy, and in vitro fluconazole liberation curves of PLGA beads were inspected via high-performance liquid chromatography. When we implanted the drug-incorporated beads into the bone cavity of rabbits, we found that a high level of fluconazole (beyond the minimum therapeutic concentration [MTC]) was released for more than 49 d in vivo. Our results indicate that compression-molded PLGA/fluconazole beads have potential applications in treating bone infections.
Evaluation of the Properties, Gas Permeability, and Selectivity of Mixed Matrix Membrane Based on Polysulfone Polymer Matrix Incorporated with KIT-6 Silica
Authors: Ding Sie Hao , Ng Tiffany Yit Siew , Chew Thiam Leng , Oh Pei Ching , Ahmad Abdul Latif , Ho Chii-Dong
Published: October 2019

Mixed matrix membranes (MMMs) separation is a promising technology for gas permeation and separation involving carbon dioxide (CO2). However, finding a suitable type of filler for the formation of defect-free MMMs with enhancement in gas permeability remains a challenge. Current study focuses on synthesis of KIT-6 silica and followed by the incorporation of KIT-6 silica as filler into polysulfone (PSF) polymer matrix to fabricate MMMs, with filler loadings of 0–8 wt %. The effect of KIT-6 incorporation on the properties of the fabricated MMMs was evaluated via different characterization techniques. The MMMs were investigated for gas permeability and selectivity with pressure difference of 5 bar at 25 °C. KIT-6 with typical rock-like morphology was synthesized. Incorporation of 2 wt % of KIT-6 into PSF matrix produced MMMs with no void. When KIT-6 loadings in the MMMs were increased from 0 to 2 wt %, the CO2 permeability increased by ~48%, whereas the ideal CO2/CH4 selectivity remained almost constant. However, when the KIT-6 loading in PSF polymer matrix was more than 2 wt %, the formation of voids in the MMMs increased the CO2 permeability but sacrificed the ideal CO2/CH4 selectivity. In current study, KIT-6 was found to be potential filler for PSF matrix under controlled KIT-6 loading for gas permeation.
Study on Preparation and Separation and Adsorption Performance of Knitted Tube Composite β-Cyclodextrin/Chitosan Porous Membrane
Authors: Tang Qian , Li Nana , Lu Qingchen , Wang Xue , Zhu Yaotian
Published: October 2019

In order to obtain membranes with both organic separation and adsorption functions, knitted tube composite β-cyclodextrin/chitosan (β-CD/CS) porous membranes were prepared by the non-solvent induced phase separation (NIPS) method using CS and β-CD as a membrane-forming matrix, glutaraldehyde as crosslinking agent to improve water stability, and knitted tube as reinforcement to enhance the mechanical properties. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), contact angle, water flux, bovine serum albumin (BSA) rejection and tensile test were carried out. The FTIR demonstrated that the β-CD and CS had been successfully crosslinked. With the crosslinking time increased, the membrane structure became denser, the contact angle and the rejection rate increased, while the water flux decreased. The strength and elongation at a break were 236 and 1.7 times higher than these of bare β-CD/CS porous membranes, respectively. The strength of crosslinking membranes increased further. The adsorption performance of composite membranes was investigated for the removal of phenolphthalein (PP) from aqueous solution. The adsorption process followed the Langmuir isotherm model, and the kinetic behavior was accorded with the Double constant equation and the Elovich equation. The adsorption mechanism could be explained by the synergistic effect of host-guest interaction from β-cyclodextrin, non-uniform diffusion and porous network capture.
Fabrication and Properties of Superhydrophobic Waterborne Polyurethane Composites with Micro-Rough Surface Structure Using Electrostatic Spraying
Authors: Wang Fangfang , Feng Lajun , Li Guangzhao , Zhai Zhe , Ma Huini , Deng Bo , Zhang Shengchao
Published: October 2019

Waterborne polyurethane (WPU) coatings hold advantages of good toughness, low cost and environmental protection. However, the low water contact angle (WCA), poor wear and corrosion resistance make them unsuitable for application in the superhydrophobic coatings such as antipollution flashover coatings for transmission lines, self-cleaning coatings for outdoor equipment and waterproof textiles. A series of superhydrophobic WPU composites (SHWPUCs) with micro-rough surface structure was prepared by electrostatic spraying nano-SiO2 particles on WPU composites with low surface energy. It showed that as the hydrophobic system content rose the WCAs of the composites first increased and then remained stationary; however, the adhesion and corrosion resistance first increased and then decreased. An appropriate addition of the hydrophobic system content would lead to a dense coating structure, but an excessive addition could increase the interfaces in the coating and then reduce the coating performance. When the mass ratio of the WPU dispersion, polytetrafluoroethylene (PTFE) particles and modified polydimethylsiloxane was 8:0.3:0.4, 10 g/m2 nano-SiO2 particles were sprayed on the uncured coating surface to construct the SHWPUC with a WCA of 156°. Compared with pure WPU coating, its adhesion and corrosion resistance increased by 12.5% and one order of magnitude, respectively; its wear rate decreased by 88.8%.
Using γ-Ray Polymerization-Induced Assemblies to Synthesize Polydopamine Nanocapsules
Authors: Jiang Wenwen , Zhang Xinyue , Luan Yafei , Wang Rensheng , Liu Hanzhou , Li Dan , Hu Liang
Published: October 2019

This work reports a simple and robust strategy for synthesis of polydopamine nanocapsules (PDA NCs). First, polymer assemblies were synthesized by a γ-ray-induced liquid–liquid (H2O–acrylate) interface polymerization strategy, in the absence of any surfactants. 1H nuclear magnetic resonance analysis and molecular dynamics simulation reveal that the generation of polymer assemblies largely depends on the hydrophilicity of acrylate and gravity of the oligomers at the interface. By virtue of the spherical structure and mechanic stability of the polymer assemblies, PDA NCs are next prepared by the interfacial polymerization of dopamine onto the assemblies, followed by the removal of templates by using ethanol. The polydopamine nanocapsules are shown to load and release ciprofloxacin (CIP, a model drug), such that the CIP-loaded PDA NCs are able to inhibit the growth of Escherichia coli.