bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic and Probiotic Characteristics of Urolithin A Producing Enterococcus faecium FUA027
Authors: Xia Mengjie , Mu Shuting , Fang Yaowei , Zhang Xiaomeng , Yang Guang , Hou Xiaoyue , He Fuxiang , Zhao Yaling , Huang Yichen , Zhang Wei , Shen Juan , Liu Shu
Published: March 2023

Enterococcus faecium FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of E. faecium FUA027 were evaluated through whole-genome sequence analysis and phenotypic assays. The chromosome size of this strain was 2,718,096 bp, with a GC content of 38.27%. The whole-genome analysis revealed that the genome contained 18 antibiotic resistance genes and seven putative virulence factor genes. E. faecium FUA027 does not contain plasmids and mobile genetic elements (MGEs), and so the transmissibility of antibiotic resistance genes or putative virulence factors should not occur. Phenotypic testing further indicated that E. faecium FUA027 is sensitive to clinically relevant antibiotics. In addition, this bacterium exhibited no hemolytic activity, no biogenic amine production, and could significantly inhibit the growth of the quality control strain. In vitro viability was >60% in all simulated gastrointestinal environments, with good antioxidant activity. The study results suggest that E. faecium FUA027 has the potential to be used in industrial fermentation for the production of urolithin A.
Impact of Milk Thermization on the Quality Characteristics of P.D.O. “Canestrato Pugliese” Ovine Hard Cheese
Authors: Natrella Giuseppe , Gambacorta Giuseppe , Squeo Giacomo , Faccia Michele
Published: March 2023

The use of raw milk is compulsory in the manufacturing process of most of the European protected designation of origin (PDO) cheeses but, for ovine products, it is often responsible for faulty productions. Since pasteurization is hardly compatible with the PDO concept, a milder treatment (thermization) is allowed in some cases. An investigation was undertaken to assess the effect of thermization on the overall quality of Canestrato Pugliese, a PDO ovine hard cheese of Southern Italy that can be manufactured exclusively from raw milk. Three types of cheese were produced using raw, mild-thermized and high-thermized milk inoculated with a thermophilic commercial starter. The results demonstrated that the heat treatment did not cause remarkable differences in the gross composition, but the microbiological profiles had some differences despite the use of the selected starter. The raw milk cheese contained higher levels (0.5–1 log units) of mesophilic lactobacilli, total viables, total coliforms and enterococci with respect to the thermized counterparts, with the high-thermized cheese showing the lowest levels; these microbiological differences fitted well with the higher content and the different High Performance Liquid Chromatography (HPLC) pattern of soluble nitrogen. The sensory analysis revealed that the thermized cheeses lost some typical sensory characteristics, probably as a consequence of the reduced indigenous microbiota populations. It was concluded that milk thermization could be applied to Canestrato Pugliese manufacturing only together with the development and use of an autochthonous starter.
Identification, Typing and Drug Resistance of Cronobacter spp. in Powdered Infant Formula and Processing Environment
Authors: Li Hongxuan , Fu Shiqian , Song Danliangmin , Qin Xue , Zhang Wei , Man Chaoxin , Yang Xinyan , Jiang Yujun
Published: March 2023

Cronobacter spp. is a food-borne pathogenic microorganism that can cause serious diseases such as meningitis, sepsis, and necrotizing colitis in infants and young children. Powdered infant formula (PIF) is one of the main contamination routes, in which the processing environment is an important source of pollution. In this investigation, 35 Cronobacter strains isolated from PIF and its processing environment were identified and typed by 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 sequence types were obtained, and three new sequence types were isolated for the first time. The antibiotic resistance was analyzed, showing that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Multi-drug resistant strains accounted for 68.57% of the total, among which Cronobacter strains with the strongest drug resistance reached 13 multiple drug resistance. Combined with transcriptomics, 77 differentially expressed genes related to drug resistance were identified. The metabolic pathways were deeply excavated, and under the stimulation of antibiotic conditions, Cronobacter strains can activate the multidrug efflux system by regulating the expression of chemotaxis-related genes, thus, secreting more drug efflux proteins to enhance drug resistance. The study of drug resistance of Cronobacter and its mechanism has important public health significance for the rational selection of existing antibacterial drugs, the development of new antibacterial drugs to reduce the occurrence of bacterial resistance, and the control and treatment of infections caused by Cronobacter.
Frozen Ready-to-(h)eat Meals: Evolution of Their Quality during a Real-Time Short Shelf Life
Authors: Dottori Ilenia , Urbani Stefania , Sordini Beatrice , Servili Maurizio , Selvaggini Roberto , Veneziani Gianluca , Ranucci David , Taticchi Agnese , Esposto Sonia
Published: March 2023

The purpose of this experimentation was to study the evolution of the quality of two types of blast-frozen ready-to-(h)eat meals, tortellini and a vegetable soup, during a short shelf life of 70 days. The analyses, performed in order to identify any variations resulting either from the freezing process or from the subsequent storage, carried out at the temperatures of −30 °C and −18 °C, respectively, examined the consistency of the tortellini and the soup, the acidity and the peroxide value of the oil extracted from them, the phenols and carotenoids present in the soup, the volatile compounds in the tortellini and the soup, and a sensory analysis of both products. The results showed that, during the 70 days of shelf life, there was no variation in the texture of the tortellini, but there were changes in the consistency of the soup, which decreased as the days of storage went on. Furthermore, statistically significant increases (p < 0.05) in the acidity and in the peroxide value of the oil of the soup were observed during the storage period; however, no statistically significant difference (p > 0.05) in the peroxide value of the oil of the tortellini was found. Moreover, no quantitative changes were observed in the phenolic compounds and carotenoids in the soup or in the volatile substances of either product. Finally, the sensory analysis confirmed, together with the chemical data, that the blast-freezing process adopted was suitable to maintain the good quality of these fresh meals, even if some technical modifications (in particular, lower freezing temperatures) should be adopted to improve the final quality of the products.
The Environment, Farm Animals and Foods as Sources of Clostridioides difficile Infection in Humans
Authors: Bolton Declan , Marcos Pilar
Published: March 2023

The recent discovery of the same Clostridioides difficile ribotypes associated with human infection in a broad range of environments, animals and foods, coupled with an ever-increasing rate of community-acquired infections, suggests this pathogen may be foodborne. The objective of this review was to examine the evidence supporting this hypothesis. A review of the literature found that forty-three different ribotypes, including six hypervirulent strains, have been detected in meat and vegetable food products, all of which carry the genes encoding pathogenesis. Of these, nine ribotypes (002, 003, 012, 014, 027, 029, 070, 078 and 126) have been isolated from patients with confirmed community-associated C. difficile infection (CDI). A meta-analysis of this data suggested there is a higher risk of exposure to all ribotypes when consuming shellfish or pork, with the latter being the main foodborne route for ribotypes 027 and 078, the hypervirulent strains that cause most human illnesses. Managing the risk of foodborne CDI is difficult as there are multiple routes of transmission from the farming and processing environment to humans. Moreover, the endospores are resistant to most physical and chemical treatments. The most effective current strategy is, therefore, to limit the use of broad-spectrum antibiotics while advising potentially vulnerable patients to avoid high-risk foods such as shellfish and pork.
Functional Ingredients and Additives from Lemon by-Products and Their Applications in Food Preservation: A Review
Authors: Magalhães Daniela , Vilas-Boas Ana A. , Teixeira Paula , Pintado Manuela
Published: March 2023

Citrus trees are among the most abundant fruit trees in the world, with an annual production of around 124 million tonnes. Lemons and limes are among the most significant contributors, producing nearly 16 million tonnes per year. The processing and consumption of citrus fruits generates a significant amount of waste, including peels, pulp, seeds, and pomace, which represents about 50% of the fresh fruit. Citrus limon (C. limon) by-products are composed of significant amounts of bioactive compounds, such as phenolic compounds, carotenoids, vitamins, essential oils, and fibres, which give them nutritional value and health benefits such as antimicrobial and antioxidant properties. These by-products, which are typically discarded as waste in the environment, can be explored to produce new functional ingredients, a desirable approach from a circular economy perspective. The present review systematically summarizes the potential high-biological-value components extracted from by-products to achieve a zero-waste goal, focusing on the recovery of three main fractions: essential oils, phenolic compounds, and dietary fibres, present in C. limon by-products, and their applications in food preservation.
3-Phenyllactic Acid and Polyphenols Are Substances Enhancing the Antibacterial Effect of Methylglyoxal in Manuka Honey
Authors: Thierig Marcus , Raupbach Jana , Wolf Diana , Mascher Thorsten , Subramanian Kannan , Henle Thomas
Published: March 2023

Manuka honey is known for its unique antibacterial activity, which is due to methylglyoxal (MGO). After establishing a suitable assay for measuring the bacteriostatic effect in a liquid culture with a time dependent and continuous measurement of the optical density, we were able to show that honey differs in its growth retardingeffect on Bacillus subtilis despite the same content of MGO, indicating the presence of potentially synergistic compounds. In model studies using artificial honey with varying amounts of MGO and 3-phenyllactic acid (3-PLA), it was shown that 3-PLA in concentrations above 500 mg/kg enhances the bacteriostatic effect of the model honeys containing 250 mg/kg MGO or more. It has been shown that the effect correlates with the contents of 3-PLA and polyphenols in commercial manuka honey samples. Additionally, yet unknown substances further enhance the antibacterial effect of MGO in manuka honey. The results contribute to the understanding of the antibacterial effect of MGO in honey.
Effect of Marjoram Leaf Powder Addition on Nutritional, Rheological, Textural, Structural, and Sensorial Properties of Extruded Rice Noodles
Authors: Vishwakarma Siddharth , Mandliya Shubham , Dalbhagat Chandrakant , Majumdar Jayshree , Mishra Hari
Published: March 2023

Food-to-food fortification is an emerging technique to enrich the micronutrients in foods. Pertaining to this technique, noodles could also be fortified with natural fortificants. In this study, marjoram leaf powder (MLP) at a level of 2–10% was used as a natural fortificant to produce fortified rice noodles (FRNs) through an extrusion process. The MLP addition caused a significant increase in the iron, calcium, protein, and fiber in the FRNs. The noodles had a lower whiteness index than unfortified noodles but had a similar water absorption index. The water solubility index increased significantly due to the higher water retention ability of MLP. A rheological study showed a minimal effect of fortification on the gelling strength of the FRNs at lower levels. The microstructural studies found incremental cracks, which facilitated a lower cooking time and hardness but had an insignificant effect on the cooked noodle texture. Fortification improved the total phenolic content, antioxidant capacity, and total flavonoid content. However, no significant changes in bonds were observed, but a reduction in the noodles’ crystallinity could be seen. The sensory analysis of the noodles reflected a higher acceptability of the 2–4% MLP fortified samples compared to the others. Overall, the MLP addition improved the nutritional content, antioxidant activity, and the cooking time but slightly affected the rheological, textural, and color properties of the noodles.
Comparison of Chemical and Sensory Profiles between Cabernet Sauvignon and Marselan Dry Red Wines in China
Authors: Song Xixian , Yang Weixi , Qian Xu , Zhang Xinke , Ling Mengqi , Yang Li , Shi Ying , Duan Changqing , Lan Yibin
Published: March 2023

The differences in chemical and sensory characteristics between Marselan and Cabernet Sauvignon in China were investigated with gas chromatography–mass spectrometry (GC-MS) and high-performance liquid chromatography–triple quadrupole mass spectrometry (HPLC-QqQ-MS/MS), combined with color parameters and sensory data. The paired t-test results showed that terpenoids, higher alcohols, and aliphatic lactones were significantly different according to the grape variety. Meanwhile, terpenoids could be considered as marker aroma compounds to distinguish Marselan wines from Cabernet Sauvignon, which could explain the distinct floral note in Marselan wines. The mean concentrations of the mv-vsol, mv-vgol, mv-vcol, mvC-vgol, mv-v(e)cat, mvC-v(e)cat, mv-di(e)cat, and cafA were higher in Marselan wines than Cabernet Sauvignon wines, and these compounds might confer Marselan wines with a deeper color, more red hue, and higher tannin quality. The phenolic profiles of Marselan and Cabernet Sauvignon wines were influenced by the winemaking process, mitigating the varietal differences. As for sensory evaluation, the intensities of herbaceous, oak, and astringency of Cabernet Sauvignon were more pronounced than Marselan, whereas the Marselan wines were characterized by a high color intensity and more redness, together with floral, sweet, and roasted sweet potato attributes, and tannin roughness.
Percolation on Fractal Networks: A Survey
Authors: Cruz Miguel-Ángel Martínez , Ortiz Julián Patiño , Ortiz Miguel Patiño , Balankin Alexander
Published: March 2023

The purpose of this survey is twofold. First, we survey the studies of percolation on fractal networks. The objective is to assess the current state of the art on this topic, emphasizing the main findings, ideas and gaps in our understanding. Secondly, we try to offer guidelines for future research. In particular, we focus on effects of fractal attributes on the percolation in self-similar networks. Some challenging questions are outlined.
Certain Properties and Applications of Δh Hybrid Special Polynomials Associated with Appell Sequences
Authors: Alyusof Rabab , Wani Shahid Ahmmad
Published: March 2023

The development of certain aspects of special polynomials in line with the monomiality principle, operational rules, and other properties and their aspects is obvious and indisputable. The study presented in this paper follows this line of research. By using the monomiality principle, new outcomes are produced, and their differential equation and series representation is obtained, which are important in several branches of mathematics and physics. Thus, in line with prior facts, our aim is to introduce the Δh hybrid special polynomials associated with Hermite polynomials denoted by ΔhHQm(u,v,w;h). Further, we obtain some well-known main properties and explicit forms satisfied by these polynomials.
Antibacterial and Anti-Acne Activity of Benzoyl Peroxide Nanoparticles Incorporated in Lemongrass Oil Nanoemulgel
Authors: Eid Ahmad M. , Naseef Hani , Jaradat Nidal , Ghanim Lina , Moqadeh Roaa , Yaseen Miasar
Published: March 2023

Purpose: The goal of this study was to make Benzoyl Peroxide (BPO) nanoemulgel to improve its ability to kill bacteria. BPO has trouble getting into the skin, being absorbed by the skin, staying stable, and being spread out. Methods: A BPO nanoemulgel formulation was prepared by combining BPO nanoemulsion with Carbopol hydrogel. The drug was tested for solubility in various oils and surfactants in order to select the best oil and surfactant for the drug, and then the drug nanoemulsion formulation was prepared using a self-nano-emulsifying technique with Tween 80, Span 80, and lemongrass oil. The drug nanoemulgel was looked at in terms of its particle size, polydispersity index (PDI), rheological behavior, drug release, and antimicrobial activity. Results: Based on the solubility test results, lemongrass oil was the best solubilizing oil for drugs, while Tween 80 and Span 80 demonstrated the highest solubilizing ability for drugs among the surfactants. The optimum self-nano-emulsifying formulation had particle sizes of less than 200 nm and a PDI of close to zero. The results showed that incorporating the SNEDDS formulation of the drug with Carbopol at various concentrations did not cause a significant change in the particle size and PDI of the drug. The zeta potential results for drug nanoemulgel were negative, with more than 30 mV. All nanoemulgel formulations exhibited pseudo-plastic behavior, with 0.4% Carbopol exhibiting the highest release pattern. The drug nanoemulgel formulation worked better against bacteria and acne than the product on the market. Conclusion: Nanoemulgel is a promising way to deliver BPO because it makes the drug more stable and increases its ability to kill bacteria.
Modeling and Optimization of the Adsorption of Cr (VI) in a Chitosan-Resole Aerogel Using Response Surface Methodology
Authors: Flores-Gómez Jean , Romero-Arellano Victor Hugo , Vazquez-Lepe Milton , Martínez-Gómez Álvaro de Jesús , Morales-Rivera Juan
Published: March 2023

In this paper, a model for Cr (VI) removal and optimization was made using a novel aerogel material, chitosan-resole CS/R aerogel, where a freeze-drying and final thermal treatment was employed to fabricate the aerogel. This processing ensures a network structure and stability for the CS, despite the non-uniform ice growth promoted by this process. Morphological analysis indicated a successful aerogel elaboration process., FTIR spectroscopy corroborated the aerogel precursor’s identity and ascertained chemical bonding after adsorption. Owing to the variability of formulations, the adsorption capacity was modeled and optimized using computational techniques. The response surface methodology (RSM), based on the Box–Behnken design using three levels, was used to calculate the best control parameters for the CS/R aerogel: the concentration at %vol (50–90%), the initial concentration of Cr (VI) (25–100 mg/L), and adsorption time (0.3–4 h). Analysis of variance (ANOVA) and 3D graphs reveal that the CS/R aerogel concentration and adsorption time are the main parameters that influence the initial concentration of CS/R aerogel metal-ion uptake. The developed model successfully describes the process with a correlation coefficient of R2 = 0.96 for the RSM. The model obtained was optimized to find the best material design proposal for Cr (VI) removal. Numerical optimization was used and showed superior Cr (VI) removal (94.4%) under conditions of a CS/R aerogel concentration of 87/13 %vol, with an initial concentration of Cr (VI) of 31 mg/L, and an adsorption time of 3.02 h. These results suggest that the proposed computational model can obtain an effective and viable model for CS material processing and for optimization of the uptake of this metal.
Introduction: The History of the “Balkan Family”
Authors: Todorova Maria
Published: March 2023

In 1996, the freshly created journal “The History of the Family” devoted its fourth issue to the Balkan Family [...]
Short Insertion and Deletion Discoveries via Whole-Genome Sequencing of 101 Thoroughbred Racehorses
Authors: Tozaki Teruaki , Ohnuma Aoi , Kikuchi Mio , Ishige Taichiro , Kakoi Hironaga , Hirota Kei-ichi , Takahashi Yuji , Nagata Shun-ichi
Published: March 2023

Thoroughbreds are some of the most famous racehorses worldwide and are currently animals of high economic value. To understand genomic variability in Thoroughbreds, we identified genome-wide insertions and deletions (INDELs) and obtained their allele frequencies in this study. INDELs were obtained from whole-genome sequencing data of 101 Thoroughbred racehorses by mapping sequence reads to the horse reference genome. By integrating individual data, 1,453,349 and 113,047 INDELs were identified in the autosomal (1–31) and X chromosomes, respectively, while 18 INDELs were identified on the mitochondrial genome, totaling 1,566,414 INDELs. Of those, 779,457 loci (49.8%) were novel INDELs, while 786,957 loci (50.2%) were already registered in Ensembl. The sizes of diallelic INDELs ranged from −286 to +476, and the majority, 717,736 (52.14%) and 220,672 (16.03%), were 1-bp and 2-bp variants, respectively. Numerous INDELs were found to have lower frequencies of alternative (Alt) alleles. Many rare variants with low Alt allele frequencies (<0.5%) were also detected. In addition, 5955 loci were genotyped as having a minor allele frequency of 0.5 and being heterogeneous genotypes in all the horses. While short-read sequencing and its mapping to reference genome is a simple way of detecting variants, fake variants may be detected. Therefore, our data help to identify true variants in Thoroughbred horses. The INDEL database we constructed will provide useful information for genetic studies and industrial applications in Thoroughbred horses, including a gene-editing test for gene-doping control and a parentage test using INDELs for horse registration and identification.
Causal Association between Iritis or Uveitis and Glaucoma: A Two-Sample Mendelian Randomisation Study
Authors: Seo Je Hyun , Lee Young
Published: March 2023

Recent studies have suggested an association between iritis or uveitis and glaucoma. This study investigated the causal relationship between glaucoma and iritis and uveitis as exposures in a multi-ethnic population. Single-nucleotide polymorphisms associated with exposures to iritis and uveitis from the genome-wide association study (GWAS) data of Biobank Japan (BBJ) and the meta-analysis data from BBJ and UK Biobank (UKB) were used as instrumental variables (IVs). The GWAS dataset for glaucoma was extracted from the meta-analysis data (n = 240,302) of Genetic Epidemiology Research in Adult Health and Aging and UKB. The casual estimates were assessed with a two-sample Mendelian randomisation (MR) test using the inverse-variance-weighted (IVW) method, weighted median method, MR–Egger method, and MR-Pleiotropy Residual Sum and Outlier test. The IVW method revealed a significant causal association between iritis and glaucoma using IVs (p < 5.0 × 10−8) from the East Asian population (n = 2) (odds ratio [OR] = 1.01, p = 0.017), a significant association between iritis exposures (p < 5.0 × 10−8) in the multi-ethnic population (n = 11) (OR = 1.04, p = 0.001), and a significant causal association between uveitis exposures (n = 10 with p < 5.0 × 10−8) and glaucoma in the multi-ethnic population (OR = 1.04, p = 0.001). Iritis and uveitis had causal effects on glaucoma risk based on IVs from the multi-ethnic population. These findings imply that the current classifications of uveitic glaucoma and open-angle glaucoma overlap, indicating the need for further investigating these complex relationships.
Molecular Phylogenetic Relationships and Unveiling Novel Genetic Diversity among Slow and Pygmy Lorises, including Resurrection of Xanthonycticebus intermedius
Authors: Blair Mary , Cao Giang , López-Nandam Elora , Veronese-Paniagua Daniel , Birchette Mark , Kenyon Marina , Md-Zain Badrul , Munds Rachel , Nekaris K. , Nijman Vincent , Roos Christian , Thach Hoàng , Sterling Eleanor , Le Minh
Published: March 2023

Genetic analysis of historical museum collections presents an opportunity to clarify the evolutionary history of understudied primate groups, improve taxonomic inferences, and inform conservation efforts. Among the most understudied primate groups, slow and pygmy lorises (genera Nycticebus and Xanthonycticebus) are nocturnal strepsirrhines found in South and Southeast Asia. Previous molecular studies have supported five species, but studies using morphological data suggest the existence of at least nine species. We sequenced four mitochondrial loci, CO1, cytb, d-loop, and ND4, for a total of 3324 aligned characters per sample from 41 historical museum specimens for the most comprehensive geographic coverage to date for these genera. We then combined these sequences with a larger dataset composed of samples collected in Vietnam as well as previously published sequences (total sample size N = 62). We inferred phylogenetic relationships using Bayesian inference and maximum likelihood methods based on data from each locus and on concatenated sequences. We also inferred divergence dates for the most recent common ancestors of major lineages using a BEAST analysis. Consistent with previous studies, we found support for Xanthonycticebus pygmaeus as a basal taxon to the others in the group. We also confirmed the separation between lineages of X. pygmaeus from northern Vietnam/Laos/China and southern Vietnam/Cambodia and included a taxonomic revision recognizing a second taxon of pygmy loris, X. intermedius. Our results found support for multiple reciprocally monophyletic taxa within Borneo and possibly Java. The study will help inform conservation management of these trade-targeted animals as part of a genetic reference database for determining the taxonomic unit and provenance of slow and pygmy lorises confiscated from illegal wildlife trade activities.
Genome-Wide Associations and Confirmatory Meta-Analyses in Diabetic Retinopathy
Authors: Yu Xinting , Rong Shisong
Published: March 2023

The present study aimed to summarize and validate the genomic association signals for diabetic retinopathy (DR), proliferative DR, and diabetic macular edema/diabetic maculopathy. A systematic search of the genome-wide association study (GWAS) catalog and PubMed/MELINE databases was conducted to curate a comprehensive list of significant GWAS discoveries. The top signals were then subjected to meta-analysis using established protocols. The results indicate the need for improved consensus among DR GWASs, highlighting the importance of validation efforts. A subsequent meta-analysis confirmed the association of two SNPs, rs4462262 (ZWINT-MRPS35P3) (odds ratio = 1.38, p = 0.001) and rs7903146 (TCF7L2) (odd ratio = 1.30, p < 0.001), with DR in independent populations, strengthening the evidence of their true association. We also compiled a list of candidate SNPs for further validation. This study highlights the importance of consistent validation and replication efforts in the field of DR genetics. The two identified gene loci warrant further functional investigation to understand their role in DR pathogenesis.
Genome-Wide Detection and Analysis of Copy Number Variation in Anhui Indigenous and Western Commercial Pig Breeds Using Porcine 80K SNP BeadChip
Authors: Xu Chengliang , Zhang Wei , Jiang Yao , Zhou Mei , Liu Linqing , Su Shiguang , Li Xueting , Wang Chonglong
Published: March 2023

Copy number variation (CNV) is an important class of genetic variations widely associated with the porcine genome, but little is known about the characteristics of CNVs in foreign and indigenous pig breeds. We performed a genome-wide comparison of CNVs between Anhui indigenous pig (AHIP) and Western commercial pig (WECP) breeds based on data from the Porcine 80K SNP BeadChip. After analysis using the PennCNV software, we detected 3863 and 7546 CNVs in the AHIP and WECP populations, respectively. We obtained 225 (loss: 178, gain: 47) and 379 (loss: 293, gain: 86) copy number variation regions (CNVRs) randomly distributed across the autosomes of the AHIP and WECP populations, accounting for 10.90% and 22.57% of the porcine autosomal genome, respectively. Functional enrichment analysis of genes in the CNVRs identified genes related to immunity (FOXJ1, FOXK2, MBL2, TNFRSF4, SIRT1, NCF1) and meat quality (DGAT1, NT5E) in the WECP population; these genes were a loss event in the WECP population. This study provides important information on CNV differences between foreign and indigenous pig breeds, making it possible to provide a reference for future improvement of these breeds and their production performance.
Density-Driven Convection for CO2 Solubility Trapping in Saline Aquifers: Modeling and Influencing Factors
Authors: Chen Yizhen , Chen Suihong , Li Didi , Jiang Xi
Published: March 2023

Industrial development has significantly increased the concentration of CO2 in the atmosphere, resulting in the greenhouse effect that harms the global climate and human health. CO2 sequestration in saline aquifers is considered to be one of the efficient ways to eliminate atmospheric CO2 levels. As an important mechanism, the solubility trapping greatly determines the efficiency of CO2 sequestration in saline aquifers, and this depends, in turn, on the density-driven convection that occurs during the sequestration. Density-driven convection is influenced by multiple factors. However, existing discussions on some of these influential factors are still ambiguous or even reach contradictory conclusions. This review summarizes the common modeling approaches and the influence of factors on density-driven convection. We suggest that saline aquifers with high values of depth, permeability, pH, and SO2 impurity concentration are the ideal CO2 sequestration sites. A certain degree of porosity, fractures, stratification, slope, hydrodynamic dispersion, background flow, and formation pressure are also considered advantageous. Meanwhile, the geological formation of the Permian White Rim Sandstone or carbonate is important, but it should not contain brine with excessive viscosity and salinity. Finally, we discuss the contents in need of further research.
Feasibility of a 12-Month Follow-Up in Swiss Older Adults after Post-Acute Care in Nursing Homes—A Pilot Study
Authors: Gagesch Michael , Hüni Andreas , Geschwindner Heike , Abderhalden Lauren A. , Lang Wei , Bieri-Brüning Gaby , Bischoff-Ferrari Heike A.
Published: March 2023

(1) Background: Post-acute care (PAC) aims to support functional recovery in older adults after acute hospitalization in order to regain a sufficient level of self-care facilitating their return home. However, the long-term outcomes of PAC are understudied due to challenges in recording a follow-up. We aimed to investigate the feasibility of a 12-month follow-up after PAC in Swiss nursing homes, examining practicability and potential factors influencing participation rate. (2) Methods: Collection of one-year follow-up data among 140 eligible patients after PAC in nursing homes was attempted. Patients were recruited using letters and phone calls between August and December 2017. We compared baseline data of all initial PAC patients with those who declined participation in the follow-up to identify factors potentially influencing participation. (3) Results: Overall mortality at 12 months was 25% (n = 35 of 140). Of the 105 survivors, 53 (50%) refused participation, 26 (25%) were interviewed, and 26 (25%) were lost to follow-up. Comparison of baseline characteristics between participants and objectors indicated significant statistical differences in Mini-Mental State Examination (MMSE) scores (participants mean of 26.0 [SD 3.92] vs. objectors mean of 23.5 points [SD 4.40], p = 0.015). Further, logistic regression showed statistically significantly greater odds of participation (OR 1.25 [95% CI 1.06–1.48]) for each point increase in MMSE scores. (4) Conclusions: Long-term follow-up studies in older adults after PAC are challenging due to high mortality and dropout rates. Of note, intact cognitive function at baseline was associated with a higher willingness to participate in a follow-up phone interview. The assessment of cognitive function should be considered when estimating the participation rate in older patients.
Humane and Comprehensive Management of Challenging Behaviour in Health and Social Care: Cross-Sectional Study Testing Newly Developed Instrument
Authors: Tölli Sirpa , Kontio Raija , Partanen Pirjo , Terkamo-Moisio Anja , Häggman-Laitila Arja
Published: March 2023

Background: Management of challenging behaviour causes victimization and violates the human rights of service users in psychiatric and long-term settings for people having mental health issues and learning disabilities. The purpose of the research was to develop and test an instrument for measuring humane behaviour management (HCMCB). The research was guided by the following questions: (1) What is the structure and content of the Human and Comprehensive management of Challenging Behaviour (HCMCB) instrument, (2) What are the psychometric properties of the HCMCB instrument, and (3) How do Finnish health and social care professionals evaluate their humane and comprehensive management of challenging behaviour? Methods: A cross-sectional study design and STROBE checklist were applied. A convenience sample of health and social care professionals (n = 233) studying at the University of Applied Sciences (n = 13) was recruited. Results: The EFA revealed a 14-factor structure and included a total of 63 items. The Cronbach’s alpha values for factors varied from 0.535 to 0.939. The participants rated their individual competence higher than leadership and organizational culture. Conclusions: HCMCB is a useful tool for evaluating competencies, leadership, and organizational practices in the context of challenging behaviour. HCMCB should be further tested in various international contexts involving challenging behaviour with large samples and longitudinal design.
Determining the Role of Employee Engagement in Nurse Retention along with the Mediation of Organizational Culture
Authors: Goyal Ridhya , Kaur Gurvinder
Published: March 2023

In today’s unpredictable environment, the rapid emergence of the COVID-19 pandemic has shaken the world and its healthcare infrastructure immensely. As nurses are the building blocks of the healthcare personnel labor market, organizations should develop tactics that aid in their retention. With a solid theoretical foundation in self-determination theory, this study aims to understand the role of employee engagement in keeping nurses in 51 hospitals in the Northern Indian region, along with the mediation of organizational culture through smart PLS. In a complementary mediation relationship with organizational culture, nurse retention is positively correlated with employee engagement.
Caregiving Self-Efficacy of the Caregivers of Family Members with Oral Cancer—A Descriptive Study
Authors: Cheng Ching-Hui , Liang Shu-Yuan , Lin Ling , Chang Tzu-Ting , Wang Tsae-Jyy , Lin Ying
Published: March 2023

In Taiwan, oral cancer is the fourth most common cause of cancer death in men. The complications and side effects of oral cancer treatment pose a considerable challenge to family caregivers. The purpose of this study was to analyze the self-efficacy of the primary family caregivers of patients with oral cancer at home. A cross-sectional descriptive research design and convenience recruiting were adopted to facilitate sampling, and 107 patients with oral cancer and their primary family caregivers were recruited. The Caregiver Caregiving Self-Efficacy Scale-Oral Cancer was selected as the main instrument to be used. The primary family caregivers’ mean overall self-efficacy score was 6.87 (SD = 1.65). Among all the dimensions, managing patient-related nutritional issues demonstrated the highest mean score (mean = 7.56, SD = 1.83), followed by exploring and making decisions about patient care (mean = 7.05, SD = 1.92), acquiring resources (mean = 6.89, SD = 1.80), and managing sudden and uncertain patient conditions (mean = 6.17, SD = 2.09). Our results may assist professional medical personnel to focus their educational strategies and caregiver self-efficacy enhancement strategies on the dimensions that scored relatively low.
Predicting Maximum Oxygen Uptake from Non-Exercise and Submaximal Exercise Tests in Paraplegic Men with Spinal Cord Injury
Authors: Lee Bum-Suk , Bae Jae-Hyuk , Choi Yu-Jin , Lee Jung-Ah
Published: March 2023

This study aimed to develop prediction equations for maximum oxygen uptake (VO2max) based on non-exercise (anthropometric) and submaximal exercise (anthropometric and physiological) variables in paraplegic men with a spinal cord injury. All participants were tested on an arm ergometer using a maximal graded exercise test. Anthropometric variables such as age, height, weight, body fat, body mass index, body fat percentage, and arm muscle mass and physiological variables such as VO2, VCO2, and heart rate at 3 and 6 min of graded exercise tests were included in the multiple linear regression analysis. The prediction equations revealed the following. Regarding non-exercise variables, VO2max was correlated with age and weight (equation R = 0.771, R2 = 0.595, SEE= 3.187). Regarding submaximal variables, VO2max was correlated with weight and VO2 and VCO2 at 6 min (equation R = 0.892, R2 = 0.796, SEE = 2.309). In conclusion, our prediction equations can be used as a cardiopulmonary function evaluation tool to estimate VO2max simply and conveniently using the anthropometric and physiological characteristics of paraplegic men with spinal cord injuries.
Child and Adolescent Mental Health during the COVID-19 Pandemic: Challenges of Psychiatric Outpatient Clinics
Authors: Mosheva Mariela , Barzilai Yael , Hertz-Palmor Nimrod , Mekori-Domachevsky Ehud , Avinir Asia , Erez Galit , Vardi Noa , Schoen Gila , Lahav Tal , Sadeh Hadar , Rapaport Michal , Dror Chen , Gizunterman Alex , Tsafrir Shlomit , Gothelf Doron , Bloch Yuval
Published: March 2023

Background: Worldwide national surveys show a rising mental health burden among children and adolescents (C&A) during COVID-19. The objective of the current study is to verify the expected rise in visits to psychiatric outpatient clinics of C&A, especially of new patients. Methods: a cross-sectional study focusing on visits as recorded in electronic medical records of eight heterogeneous C&A psychiatric outpatient clinics. The assessment was based on visits held from March to December of 2019 (before the pandemic) in comparison to visits held in 2020 (during the pandemic). Results: The number of visits was similar for both periods. However, in 2020, 17% of the visits used telepsychiatry (N = 9885). Excluding telepsychiatry reveals a monthly decrease in traditional in-person activities between 2020 and 2019 (691.6 ± 370.8 in 2020 vs. 809.1 ± 422.8 in 2019, mean difference = −117.5, t (69) = −4.07, p = 0.0002, Cohen’s d = −0.30). Acceptation of new patients declined during 2020, compared to 2019 (50.0 ± 38.2 in 2020 vs. 62.8 ± 42.9 in 2019; Z = −3.12, p = 0.002, r = 0.44). Telepsychiatry was not used for new patients. Conclusions: The activity of C&A psychiatric outpatient clinics did not rise but was guarded due to the use of telepsychiatry. The decline in visits of new patients was explained by the lack of use of telepsychiatry for these patients. This calls for expanding the use of telepsychiatry, especially for new patients.
Development of Data Labelling and Visualization Technologies for Cultural Heritage Intelligent Curation
Authors: Lee Jongwook , Shin Hyunbin , Im Seoyeon
Published: March 2023

This study aims to enable users of digitized cultural heritage artefacts to understand the correlation between artefacts and their context, discover new knowledge, and gain inspiration. We have developed data labelling and visualization technologies specific to cultural heritage so that users, such as curators, cultural heritage experts, and museum visitors, can easily and quickly access the information that they want through the correlation between artefacts. We came up with a web-based labelling tool for cultural heritage data and applied it to data on 45,692 artefacts from the National Museum of Korea. We also invented a web-based visualization tool that converts lists of attribute information in Excel spreadsheets into visualized content and edits the attributes. The meanings of thumbnails of visualized cultural heritage and that of connected lines, a search function, and visualized cultural heritage will allow users to quickly and easily access the cultural heritage information they are looking for.
Equity/Equality, Diversity and Inclusion, and Other EDI Phrases and EDI Policy Frameworks: A Scoping Review
Authors: Wolbring Gregor , Nguyen Annie
Published: March 2023

Equity, equality, diversity, inclusion, belonging, dignity, justice, accessibility, accountability, and decolonization are individual concepts used to engage with problematic social situations of marginalized groups. Phrases that put together these concepts in different ways, such as “equity, diversity and inclusion”, “equality, diversity, and inclusion”, “diversity, equity and inclusion”, “equity, diversity, inclusion, and accessibility”, “justice, equity, diversity, and inclusion”, and “equity, diversity, inclusion, and decolonization” are increasingly used, indicating that any one of these concepts is not enough to guide policy decisions. These phrases are also used to engage with problems in the workplace. Universities are one workplace where these phrases are used to improve the research, education, and general workplace climate of marginalized students, non-academic staff, and academic staff. EDI policy frameworks such as Athena SWAN and DIMENSIONS: equity, diversity, and inclusion have been also set up with the same purpose. What EDI data are generated within the academic literature focusing on EDI in the workplace, including the higher education workplace, influence the implementation and direction of EDI policies and practices within the workplace and outside. The aim of this scoping review of academic abstracts employing SCOPUS, the 70 databases of EBSCO-HOST and Web of Sciences, was to generate data that allow for a detailed understanding of the academic inquiry into EDI. The objective of this study was to map out the engagement with EDI in the academic literature by answering seven research questions using quantitative hit count manifest coding: (1) Which EDI policy frameworks and phrases are mentioned? (2) Which workplaces are mentioned? (3) Which academic associations, societies, and journals and which universities, colleges, departments, and academic disciplines are mentioned? (4) Which medical disciplines and health professionals are mentioned? (5) Which terms, phrases, and measures of the “social” are present? (6) Which technologies, science, and technology governance terms and ethics fields are present? (7) Which EDI-linked groups are mentioned and which “ism” terms? Using a qualitative thematic analysis, we aimed to answer the following research question: (8) What are the EDI-related themes present in relation to (a) the COVID-19/pandemic, (b) technologies, (c) work/life, (d) intersectionality, (e) empowerment of whom, (f) “best practices”, (g) evaluation and assessment of EDI programs, (h) well-being, and (i) health equity. We found many gaps in the academic coverage, suggesting many opportunities for academic inquiries and a broadening of the EDI research community.
Antioxidant, Antimicrobial, and Anti-Insect Properties of Boswellia carterii Essential Oil for Food Preservation Improvement
Authors: Borotová Petra , Čmiková Natália , Galovičová Lucia , Vukovic Nenad L. , Vukic Milena D. , Tvrdá Eva , Kowalczewski Przemysław Łukasz , Kluz Maciej Ireneusz , Puchalski Czeslaw , Schwarzová Marianna , Bakay Ladislav , Kačániová Miroslava
Published: March 2023

Boswellia carterii, known as frankincense, is a fragrant medicinal plant. The essential oil from this plant is often used in traditional medicine or aromatherapy. Due to its positive properties, it has potential applications as an antimicrobial agent in medicine and the food industry. The aim of this study was to evaluate the antimicrobial, anti-insect, and antioxidant activities of frankincense essential oil (FEO). The composition of volatile compounds was determined by GC/MS, and the main components were found to be α-pinene (37.0%), α-limonene (19.8%), and p-cymene (6.3%). The antioxidant activity was evaluated with DPPH and ABTS methods with the resulting inhibition of 73.88 ± 0.35% of DPPH radical (6.27 ± 0.17 TEAC) and 97.09 ± 0.48% of ABTS radical cation (5.21 ± 0.07 TEAC). The antimicrobial activity was the strongest against Candida species with inhibition zones in the range of 38.00–44.00 mm and MIC 50 and MIC 90 values of 11.72 and 12.58 μL/mL, respectively. The inhibition activity of the vapor phase was the highest against G+ and G− bacteria growing on a carrot with inhibition ranging from 65.14 to 93.67%. The anti-insect activity against O. lavaterae was determined as 100% at 100% concentration and 50% at 25% concentration. The degradation of biofilm was tested with MALDI-TOF MS, and changes in the protein profile were observed.
Horticultural Activities Participation and College Students’ Positive Mental Characters: Mediating Role of Academic Self-Efficacy
Authors: Guo Siyuan , Li Tongyu , Xue Binxia , Yang Xiuxian
Published: March 2023

In recent years, the ongoing impact of the COVID-19 epidemic, irregular closed school life and frequent online teaching have negatively impacted the mental health and academic performance of many college students. Doing horticultural activities is an effective way to promote physical and mental health and enhance academic performance. This paper explores the relationship between horticultural activities participation, academic self-efficacy and positive mental characters under the perspective of disciplinary integration, with a view to promoting the mental health status and academic performance of college students and the application of horticultural therapy on college campuses. Questionnaires such as the Positive Mental Characters Scale for Chinese College Students (PMCS-CCS) and Academic Self-Efficacy Scale (ASES) are used to investigate 160 college students from four universities in China. The results show that horticultural activity participation is significantly positively correlated with academic self-efficacy (r = 0.345; p < 0.01) and positive mental characters (r = 0.298; p < 0.01), and horticultural activity participation can positively affect positive mental characters (B = 0.135, p < 0.01). At the same time, academic self-efficacy has a partial mediating effect between horticultural activity participation and positive mental characters. Universities can actively carry out campus horticultural activities to enhance students’ horticultural activity participation, which in turn promotes academic self-efficacy and further enhances the positive psychological level of college students.