bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunomodulatory Effects of the Cyclooxygenase Inhibitor Lornoxicam on Phenotype and Function of Camel Blood Leukocytes
Authors: Hussen Jamal , Kandeel Mahmoud , Shawaf Turke , Al-Mubarak Abdullah I. A. , Al-Humam Naser A. , Almathen Faisal
Published: July 2021

(1) Background: Lornoxicam is a nonsteroidal anti-inflammatory drug (NSAID) with analgesic, antiphlogistic and antipyretic effects. The improved tolerance of lornoxicam due to the relatively shorter elimination half-life in comparison to other members of the oxicams may favor its application in the management of pain and inflammation in race dromedary camels. There are no studies conducted yet on the immunomodulatory or immunotoxilogic effect of lornoxicam in camels. Therefore, the current study aimed to evaluate the immunomodulatory effects of the cyclooxygenase inhibitor lornoxicam on some phenotypic and functional properties of camel blood leukocytes; (2) Methods: Using flow cytometry, blood leukocyte composition, monocyte phenotype, and antimicrobial functions of neutrophils and monocytes were analyzed ex vivo after a single dose injection with lornoxicam. In addition, the effect of in vitro incubation of camel blood with lornoxicam on leukocyte cell vitality and antimicrobial functions were evaluated; (3) Results: The injection of camels with a single dose of lornoxicam resulted in a significant change in their leukogram with reduced numbers of total leukocytes, neutrophils, eosinophils, monocytes, and lymphocytes. Within the lymphocyte population, the numbers of CD4+ T cells, γδ T cells, and B cells decreased significantly in blood after injection of camels with lornoxicam. In addition, injection of lornoxicam resulted in decreased abundance of major histocompatibility complex (MHC) class II molecules and increased abundance of the scavenger receptor CD163 on blood monocytes, indicating an anti-inflammatory phenotype of monocytes. Functionally, administration of lornoxicam decreased the capacity of camel neutrophils and monocytes to uptake bacteria and to produce reactive oxygen species (ROS) after bacterial stimulation. Similarly, the in vitro whole blood incubation with lornoxicam resulted in reduced phagocytosis and ROS production activity of the camel blood phagocytes. Flow cytometric analysis of cell vitality, including cell necrosis and apoptosis, revealed a pro-apoptotic effect of lornoxicam on camel leukocytes; (4) Conclusions: Lornoxicam administration, at the dose and intervals utilized herein, induces significant changes in the phenotype and function of camel blood leukocytes. The reduced cell numbers of all studied leukocyte subpopulations in lornoxicam-treated camels, which seems to be a result of enhanced cell apoptosis, indicates an inhibitory effect rather than a modulatory effect of lornoxicam on the camel immune system, which need to be considered when using lornoxicam in camel medicine.
Identification of Human SARS-CoV-2 Monoclonal Antibodies from Convalescent Patients Using EBV Immortalization
Authors: Valgardsdottir Rut , Cattaneo Irene , Napolitano Gavino , Raglio Annibale , Spinelli Orietta , Salmoiraghi Silvia , Castilletti Concetta , Lapa Daniele , Capobianchi Maria Rosaria , Farina Claudio , Golay Josee
Published: July 2021

We report the isolation of two human IgG1k monoclonal antibodies (mAbs) directed against the SARS-CoV-2 spike protein. These mAbs were isolated from two donors who had recovered from COVID-19 infection during the first pandemic peak in the Lombardy region of Italy, the first European and initially most affected region in March 2020. We used the method of EBV immortalization of purified memory B cells and supernatant screening with a spike S1/2 assay for mAb isolation. This method allowed rapid isolation of clones, with one donor showing about 7% of clones positive against spike protein, whereas the other donor did not produce positive clones out of 91 tested. RNA was extracted from positive clones 39–47 days post-EBV infection, allowing VH and VL sequencing. The same clones were sequenced again after a further 100 days in culture, showing that no mutation had taken place during in vitro expansion. The B cell clones could be expanded in culture for more than 4 months after EBV immortalization and secreted the antibodies stably during that time, allowing to purify mg quantities of each mAb for functional assays without generating recombinant proteins. Unfortunately, neither mAb had significant neutralizing activity in a virus infection assay with several different SARS-CoV-2 isolates. The antibody sequences are made freely available.
S-PRG Filler Eluate Induces Oxidative Stress in Oral Microorganism: Suppression of Growth and Pathogenicity, and Possible Clinical Application
Authors: Kono Yu , Tamura Muneaki , Cueno Marni E. , Tonogi Morio , Imai Kenichi
Published: July 2021

Controlling the oral microbial flora is putatively thought to prevent not only oral diseases, but also systemic diseases caused by oral diseases. This study establishes the antibacterial effect of the novel bioactive substance “S-PRG filler” on oral bacteria. We examined the state of oxidative stress caused by the six types of ions released in eluate from the S-PRG filler in oral bacterial cells. Moreover, we investigated the effects of these ions on the growth and pathogenicity of Gram-positive and Gram-negative bacteria. We found that the released ions affected SOD amount and hydrogen peroxide in bacterial cells insinuating oxidative stress occurrence. In bacterial culture, growth inhibition was observed depending on the ion concentration in the medium. Additionally, released ions suppressed Streptococcus mutans adhesion to hydroxyapatite, S. oralis neuraminidase activity, and Porphyromonas gingivalis hemagglutination and gingipain activity in a concentration-dependent manner. From these results, it was suggested that the ions released from the S-PRG filler may suppress the growth and pathogenicity of the oral bacterial flora. This bioactive material is potentially useful to prevent the onset of diseases inside and outside of the oral cavity, which in turn may have possible applications for oral care and QOL improvement.
In Vitro Antifungal Activity and Toxicity of Dihydrocarvone-Hybrid Derivatives against Monilinia fructicola
Authors: Díaz Katy , Werner Enrique , Besoain Ximena , Flores Susana , Donoso Viviana , Said Bastian , Caro Nelson , Vega Ernesto , Montenegro Iván , Madrid Alejandro
Published: July 2021

The aim of this study was to synthesize a series of novel and known dihydrocarvone-hybrid derivatives (2–9) and to evaluate mycelial growth activity of hybrid molecules against two strains of Monilinia fructicola, as well as their toxicity. Dihydrocarvone-hybrid derivatives have been synthesized under sonication conditions and characterized by FTIR, NMR, and HRMS. Antifungal efficacy against both strains of M. fructicola was determined by half maximal effective concentration (EC50) and toxicity using the brine shrimp lethality test (BSLT). Among the synthesized compounds, 7 and 8 showed the best activity against both strains of M. fructicola with EC50 values of 148.1 and 145.9 µg/mL for strain 1 and 18.1 and 15.7 µg/mL for strain 2, respectively, compared to BC 1000® (commercial organic fungicide) but lower than Mystic® 520 SC. However, these compounds showed low toxicity values, 910 and 890 µg/mL, respectively, compared to Mystic® 520 SC, which was highly toxic. Based on the results, these hybrid compounds could be considered for the development of more active, less toxic, and environmentally friendly antifungal agents against phytopathogenic fungi.
Risk Factors for Antimicrobial Resistance in Turkey Farms: A Cross-Sectional Study in Three European Countries
Authors: Horie Mayu , Yang Dongsheng , Joosten Philip , Munk Patrick , Wadepohl Katharina , Chauvin Claire , Moyano Gabriel , Skarżyńska Magdalena , Dewulf Jeroen , Aarestrup Frank M. , Blaha Thomas , Sanders Pascal , Gonzalez-Zorn Bruno , Wasyl Dariusz , Wagenaar Jaap A. , Heederik Dick , Mevius Dik , Schmitt Heike , Smit Lidwien A. M. , Van Gompel Liese
Published: July 2021

Food-producing animals are an important reservoir and potential source of transmission of antimicrobial resistance (AMR) to humans. However, research on AMR in turkey farms is limited. This study aimed to identify risk factors for AMR in turkey farms in three European countries (Germany, France, and Spain). Between 2014 and 2016, faecal samples, antimicrobial usage (AMU), and biosecurity information were collected from 60 farms. The level of AMR in faecal samples was quantified in three ways: By measuring the abundance of AMR genes through (i) shotgun metagenomics sequencing (n = 60), (ii) quantitative real-time polymerase chain reaction (qPCR) targeting ermB, tetW, sul2, and aph3′-III; (n = 304), and (iii) by identifying the phenotypic prevalence of AMR in Escherichia coli isolates by minimum inhibitory concentrations (MIC) (n = 600). The association between AMU or biosecurity and AMR was explored. Significant positive associations were detected between AMU and both genotypic and phenotypic AMR for specific antimicrobial classes. Beta-lactam and colistin resistance (metagenomics sequencing); ampicillin and ciprofloxacin resistance (MIC) were associated with AMU. However, no robust AMU-AMR association was detected by analyzing qPCR targets. In addition, no evidence was found that lower biosecurity increases AMR abundance. Using multiple complementary AMR detection methods added insights into AMU-AMR associations at turkey farms.
Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties
Authors: Nainu Firzan , Masyita Ayu , Bahar Muh. Akbar , Raihan Muhammad , Prova Shajuthi Rahman , Mitra Saikat , Emran Talha Bin , Simal-Gandara Jesus
Published: July 2021

Bee products have long been used in traditional healing practices to treat many types of disorders, including cancer and microbial-related diseases. Indeed, several chemical compounds found in bee products have been demonstrated to display anticancer, antibacterial, antiviral, and antiparasitic properties. With the improvement of research tools and in view of recent advances related to bee products, this review aims to provide broad yet detailed insight into the pharmaceutical prospects of bee products such as honey, propolis, bee pollen, royal jelly, bee bread, beeswax, and bee venom, in the domain of cancer and infectious disease management. Available literature confirms the efficacy of these bee products in the alleviation of cancer progression, inhibition of bacterial and viral proliferation, and mitigation of parasitic-related symptoms. With such potentials, bioactive components isolated from the bee products can be used as an alternative approach in the long-run effort to improve humans’ health at a personal and community level.
Improving Sperm Oxidative Stress and Embryo Quality in Advanced Paternal Age Using Idebenone In Vitro—A Proof-of-Concept Study
Authors: Nikitaras Victoria , Zander-Fox Deirdre , McPherson Nicole O.
Published: July 2021

Advanced paternal age is associated with increased sperm reactive oxygen species (ROS) and decreased fertilization and pregnancy rates. Sperm washing during infertility treatment provides an opportunity to reduce high sperm ROS concentrations associated with advanced paternal age through the addition of idebenone. Sperm from men aged >40 years and older CBAF1 mice (12–18 months), were treated with 5 µM and 50 µM of idebenone and intracellular and superoxide ROS concentrations assessed. Following in vitro fertilization (IVF), embryo development, blastocyst differentiation, DNA damage and cryosurvival, pregnancy and implantation rates and fetal and placental weights were assessed. Five µM of idebenone given to aged human and mouse sperm reduced superoxide concentrations ~20% (p < 0.05), while both 5 and 50 µM reduced sperm intracellular ROS concentrations in mice ~30% (p < 0.05). Following IVF, 5 µM of idebenone to aged sperm increased fertilization rates (65% vs. 60%, p < 0.05), blastocyst total, trophectoderm and inner cell mass cell numbers (73 vs. 66, 53 vs. 47 and 27 vs. 24, respectively, p < 0.01). Treatment with idebenone also increased blastocyst cryosurvival rates (96% vs. 78%, p < 0.01) and implantation rates following embryo transfer (35% vs. 18%, p < 0.01). Placental weights were smaller (107 mg vs. 138 mg, p < 0.05), resulting in a larger fetal to placental weight ratio (8.3 vs. 6.3, p = 0.07) after sperm idebenone treatment. Increased sperm ROS concentrations associated with advanced paternal age are reduced with the addition of idebenone in vitro, and are associated with improved fertilization rates, embryo quality and implantation rates after IVF.
Rice Husk Silica Liquid Protects Pancreatic β Cells from Streptozotocin-Induced Oxidative Damage
Authors: Chen Hsin-Yuan , Chiang Yi-Fen , Wang Kai-Lee , Huang Tsui-Chin , Ali Mohamed , Shieh Tzong-Ming , Chang Hsin-Yi , Hong Yong-Han , Hsia Shih-Min
Published: July 2021

Type 2 diabetes mellitus is a complex multifactorial disease characterized by insulin resistance and dysfunction of pancreatic β-cells. Rice husk silica liquid (RHSL) is derived from rice husks and has not been explored in diabetes mellitus until now. Previous studies showed that rice husk is enriched with silica, and its silica nanoparticles are higher more biocompatible. To investigate the potential protective role of RHSL on pancreatic β cells, we utilized RIN-m5F pancreatic β cells and explored RHSL effect after streptozotocin (STZ)-stimulation. The recovery effects of RHSL were evaluated using flow cytometry, Western blotting, and immunofluorescence analysis. Results of our study showed that RHSL reversed the cell viability, insulin secretion, reactive oxygen species (ROS) production, and the change of mitochondria membrane potential (ΔΨm) in STZ-treated RIN-m5F cells. Moreover, the expression of phospho-receptor-interacting protein 3 (p-RIP3) and cleaved-poly (ADP-ribose) polymerase (PARP), phospho-mammalian target of rapamycin (p-mTOR), and sequestosome-1 (p62/SQSTM1) were significantly decreased, while the transition of light chain (LC)3-I to LC3-II was markedly increased after RHSL treatment in STZ-induced RIN-m5F cells. Interestingly, using autophagy inhibitors such as 3-methyladenine (3-MA) and chloroquine (CQ) both showed an increase in cleaved-PARP protein level, indicating apoptosis induction. Taken together, this study demonstrated that RHSL induced autophagy and alleviated STZ-induced ROS-mediated apoptosis in RIN-m5F cells.
Integration of Cardiac Actin Mutants Causing Hypertrophic (p.A295S) and Dilated Cardiomyopathy (p.R312H and p.E361G) into Cellular Structures
Authors: Erdmann Constanze , Hassoun Roua , Schmitt Sebastian , Kikuti Carlos , Houdusse Anne , Mazur Antonina J. , Mügge Andreas , Hamdani Nazha , Geyer Matthias , Jaquet Kornelia , Mannherz Hans Georg
Published: July 2021

The human mutant cardiac α-actins p.A295S or p.R312H and p.E361G, correlated with hypertrophic or dilated cardiomyopathy, respectively, were expressed by the baculovirus/Sf21 insect cell system and purified to homogeneity. The purified cardiac actins maintained their native state but showed differences in Ca2+-sensitivity to stimulate the myosin-subfragment1 ATPase. Here we analyzed the interactions of these c-actins with actin-binding and -modifying proteins implicated in cardiomyocyte differentiation. We demonstrate that Arp2/3 complex and the formin mDia3 stimulated the polymerization rate and extent of the c-actins, albeit to different degrees. In addition, we tested the effect of the MICAL-1 monooxygenase, which modifies the supramolecular actin organization during development and adaptive processes. MICAL-1 oxidized these c-actin variants and induced their de-polymerization, albeit at different rates. Transfection experiments using MDCK cells demonstrated the preferable incorporation of wild type and p.A295S c-actins into their microfilament system but of p.R312H and p.E361G actins into the submembranous actin network. Transduction of neonatal rat cardiomyocytes with adenoviral constructs coding HA-tagged c-actin variants showed their incorporation into microfilaments after one day in culture and thereafter into thin filaments of nascent sarcomeric structures at their plus ends (Z-lines) except the p.E361G mutant, which preferentially incorporated at the minus ends.
LC-ESI-LTQ-Orbitrap-MS for Profiling the Distribution of Oleacein and Its Metabolites in Rat Tissues
Authors: López-Yerena Anallely , Vallverdú-Queralt Anna , Lamuela-Raventós Rosa M. , Escribano-Ferrer Elvira
Published: July 2021

The purpose of this work was to study the distribution of oleacein (OLEA) and its metabolites in rat plasma and different tissues, namely brain, heart, kidney, liver, lung, small intestine, spleen, stomach, skin, and thyroid, following the acute intake of a refined olive oil containing 0.3 mg/mL of OLEA. For this purpose, a distribution kinetics study was carried out. The plasma and tissues were collected at 1, 2, and 4.5 h after the intervention, and analyzed by LC-ESI-LTQ-Orbitrap-MS. Unmetabolized OLEA was detected in the stomach, small intestine, liver, plasma and, most notably, the heart. This finding may be useful for the development of new applications of OLEA for cardiovascular disease prevention. Noteworthy are also the high levels of hydroxytyrosol (OH-TY) and OLEA + CH3 found in the small intestine, liver, and plasma, and the detection of nine OLEA metabolites, five of them arising from conjugation reactions. Liver, heart, spleen, and lungs were the target tissues where the metabolites were most distributed. However, it is important to note that OH-TY, in our experimental conditions, was not detected in any target tissue (heart, spleen, thyroids, lungs, brain, and skin). These results shed further light on the metabolism and tissue distribution of OLEA and contribute to understanding the mechanisms underlying its effect in human health.
Therapies with Antioxidant Potential in Psoriasis, Vitiligo, and Lichen Planus
Authors: Guarneri Fabrizio , Bertino Lucrezia , Pioggia Giovanni , Casciaro Marco , Gangemi Sebastiano
Published: July 2021

Oxidative stress plays an important pathogenetic role in many chronic inflammatory diseases, including those of dermatological interest. In particular, regarding psoriasis, vitiligo, and lichen planus, excess reactive oxygen species and a decline in endogenous antioxidant systems are observed. In this regard, treatments with antioxidant properties could be appropriate therapeutic options. To date, clinical trials in dermatology on these treatments are limited. We reviewed the available studies on the efficacy of antioxidant therapies in psoriasis, vitiligo, and lichen planus. The role of herbal derivatives, vitamins, and trace elements was analyzed. The antioxidant properties of conventional therapies were also evaluated. Data from the literature suggest that antioxidants might be useful, but available studies on this topic are limited, heterogeneous, not completely standardized, and on small populations. Furthermore, in most cases, antioxidants alone are unable to induce significant clinical changes, except perhaps in mild forms, and must be used in conjunction with standard drug treatments to achieve measurable results. Further studies need to be conducted, considering larger populations and using internationally validated scales, in order to compare the results and clinical efficacy.
Application of Gas Foil Bearings in China
Authors: Hou Yu , Zhao Qi , Guo Yu , Ren Xionghao , Lai Tianwei , Chen Shuangtao
Published: July 2021

Gas foil bearing has been widely used in high-speed turbo machinery due to its oil-free, wide temperature range, low cost, high adaptability, high stability and environmental friendliness. In this paper, state-of-the-art investigations of gas foil bearings are reviewed, mainly on the development of the high-speed turbo machinery in China. After decades of development, progress has been achieved in the field of gas foil bearing in China. Small-scale applications of gas foil bearing have been realized in a variety of high-speed turbo machinery. The prospects and markets of high-speed turbo machinery are very broad. Various high-speed turbomachines with gas foil bearings have been developed. Due to the different application occasions, higher reliability requirements are imposed on the foil bearing technology. Therefore, its design principle, theory, and manufacturing technology should be adaptive to new application occasions before mass production. Thus, there are still a number of inherent challenges that must be addressed, for example, thermal management, rotor-dynamic stability and wear-resistant coatings.
Regenerative Endodontics as the Future Treatment of Immature Permanent Teeth
Authors: Zbańska Justyna , Herman Katarzyna , Kuropka Piotr , Dobrzyński Maciej
Published: July 2021

The regenerative endodontic procedure (REP) is an alternative solution for endodontic treatment of permanent teeth with incomplete root apex development. It results in angiogenesis, reinnervation, and further root formation. Indications for REP include immature permanent teeth with necrotic pulp and inflammatory lesions of the periapical tissues. The main contraindications comprise significant destruction of the tooth tissues and a lack of patient cooperation. We distinguish the following stages of this procedure: disinfection of the canal, delivery of the REP components, closure of the cavity, and follow-up appointments. For effective canal disinfection, the use of both rinsing agents and intracanal medicaments is suggested. Sodium hypochlorite and triple antibiotic paste are used most commonly. Light-activated disinfection is proposed as an alternative method. The prerequisite for the regeneration process of the pulp is the supply of its essential components: stem cells, growth factors, and scaffolds to the canal lumen. Blood clotting, platelet-rich plasma, and platelet-rich fibrin are used for this purpose. For a proper course of REP, it is also necessary to close the tooth canal tightly. For this purpose, mineral trioxide aggregate (MTA), tricalcium silicate (Biodentine), or types of glass ionomer cement are employed. The patient should attend regularly scheduled follow-up appointments and each time undergo a thorough interview, physical and radiological examination. The most important indicator of a successful REP is the continued growth of the root in length and thickness and the closure of the root apex visible on X-rays. Many different proposals for a management protocol have been published; the following paper proposes the authors’ original scheme. Regenerative endodontics is the future of the endodontic treatment of immature permanent teeth; however, it still requires a lot of research to refine and standardize the treatment protocol. The application of tissue engineering methods seems to be promising, also for mature teeth treatment.
Asynchrony Drives Plant and Animal Community Stability in Mediterranean Coastal Dunes
Authors: Bird Tania L.F. , Bar (Kutiel) Pua , Groner Elli , Bouskila Amos
Published: July 2021

Substantial evidence now suggests that a positive diversity–stability relationship exists. Yet few studies examine the facets of biodiversity that contribute to this relationship, and empirical research is predominantly conducted on grassland communities under controlled conditions. We investigate the roles of species richness, environmental condition (vegetation cover), asynchrony, and weighted population stability in driving community stability across multiple taxa. We used data from a Long-term Ecological Research project to investigate temporal stability of annual plants, beetles, reptiles, and rodents in Nizzanim Coastal Sand Dune Nature Reserve in Israel. All four taxa had a strong positive relationship between asynchrony and community stability. Only rodents showed a positive richness–stability relationship. Perennial plant cover had a significant relationship with community stability for three taxa, but the direction of the correlation varied. Asynchrony had a stronger relationship with perennial plant cover than it did with richness for both plants and beetles. We suggest that community stability is driven by asynchrony for flora as well as fauna. Stability appears to be determined by species’ interactions and their responses to the environment, and not always by diversity. This has important consequences for understanding the effects of environmental degradation on ecosystem stability and productivity, which have destabilizing consequences beyond biodiversity loss.
Changes in the Biochemical Composition and Physicochemical Properties of Apples Stored in Controlled Atmosphere Conditions
Authors: Butkeviciute Aurita , Viskelis Jonas , Viskelis Pranas , Liaudanskas Mindaugas , Janulis Valdimaras
Published: July 2021

Apples are an important component of the diet and are used in the food industry in the production of food products and beverages. The aim of the study was to determine the changes in the biochemical composition and physicochemical properties of apples stored in a controlled atmosphere. We studied the biochemical composition (sugars, ascorbic acid, soluble solids, and titratable acidity) and physicochemical properties (color coordinates, peel, and flesh firmness) in the apple samples before placing them in the controlled atmosphere chambers and at the end of the experiment 8 months later. The total content of sugars and soluble solids was found to increase in the samples of apples stored in I to VIII conditions. The study showed a decrease in titratable acidity in apple samples of all cultivars stored in I to VIII conditions. The values of C*, L*, a*, and b* co-ordinates of apple colors were evaluated. Apple samples stored in VI conditions were the lightest color, and their lightness was close to that of fresh fruit. The firmness of apple peel samples of the ‘Sampion’ cultivar stored in I and III–VI conditions increased. The study is valuable and proves that, under the studied conditions, it is possible to extend the time of the provision of apples to the consumers with minimal changes in their chemical composition and nutritional value.
Informative Biomarkers for Autism Spectrum Disorder Diagnosis in Functional Magnetic Resonance Imaging Data on the Default Mode Network
Authors: Karampasi Aikaterini S. , Savva Antonis D. , Korfiatis Vasileios Ch. , Kakkos Ioannis , Matsopoulos George K.
Published: July 2021

Effective detection of autism spectrum disorder (ASD) is a complicated procedure, due to the hundreds of parameters suggested to be implicated in its etiology. As such, machine learning methods have been consistently applied to facilitate diagnosis, although the scarcity of potent autism-related biomarkers is a bottleneck. More importantly, the variability of the imported attributes among different sites (e.g., acquisition parameters) and different individuals (e.g., demographics, movement, etc.) pose additional challenges, eluding adequate generalization and universal modeling. The present study focuses on a data-driven approach for the identification of efficacious biomarkers for the classification between typically developed (TD) and ASD individuals utilizing functional magnetic resonance imaging (fMRI) data on the default mode network (DMN) and non-physiological parameters. From the fMRI data, static and dynamic connectivity were calculated and fed to a feature selection and classification framework along with the demographic, acquisition and motion information to obtain the most prominent features in regard to autism discrimination. The acquired results provided high classification accuracy of 76.63%, while revealing static and dynamic connectivity as the most prominent indicators. Subsequent analysis illustrated the bilateral parahippocampal gyrus, right precuneus, midline frontal, and paracingulate as the most significant brain regions, in addition to an overall connectivity increment.
Comparative Analysis of Derivatization Reagents for Catecholamines and Amino Acids
Authors: Taira Shu , Ikeda Akari , Kobayashi Shoko , Shikano Hitomi , Ikeda Ryuzoh , Maejima Yuko , Horita Shoichiro , Yokoyama Jun , Shimomura Kenju
Published: July 2021

We compared four derivatization reagents to analyze catecholamines and amino acids by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. 2,4,6-Trimethylpyrylium tetrafluoroborate (TMPy), 2,4-diphenyl-pyranylium tetrafluoroborate (DPP-TFB), 4-(anthracen-9-yl)-2-fluoro-1-methylpyridin-1-ium iodide (FMP-10), and triphenyl pyrilium (TPP) were used as derivatization reagents that can specifically modify primary amines or hydroxy groups in target molecules. Three derivatization reagents, not including TPP, reacted with all target molecules. The derived catecholamines dopamine and L-DOPA, and the amino acids GABA and glycine, were efficiently ionized in comparison with non-derivatized targets. Comparative analysis indicated that TMPy and FMP-10 produced general increases in signal-to-noise ratios (S/N), whereas DPP and TPP produced specific increases in the S/N of GABA and DA. Notably, TMPy is a small molecule that efficiently reacts with target molecules due to the absence of high bulk and steric hinderance.
Packet Optical Transport Network Slicing with Hard and Soft Isolation
Authors: Barguil Samier , Lopez Alvarez Victor , Contreras Murillo Luis Miguel , Gonzalez de Dios Oscar , Alcala Alvarez Alejandro , Manso Carlos , Alemany Pol , Casellas Ramon , Martinez Ricardo , Gonzalez-Perez David , Liu Xufeng , Pulido Jose-Miguel , Fernandez-Palacios Juan Pedro , Muñoz Raul , Vilalta Ricard
Published: July 2021

Network operators have been dealing with the necessity of a dynamic network resources allocation to provide a new generation of customer-tailored applications. In that sense, Telecom providers have to migrate their BSS/OSS systems and network infrastructure to more modern solutions to introduce end-to-end automation and support the new use cases derived from the 5G adoption and transport network slices. In general, there is a joint agreement on making this transition to an architecture defined by programmable interfaces and standard protocols. Hence, this paper uses the iFusion architecture to control and program the network infrastructure. The work presents an experimental validation of the network slicing instantiation in an IP/Optical environment using a set of standard protocols and interfaces. The work provides results of the creation, modification and deletion of the network slices. Furthermore, it demonstrates the usage of standard communication protocols (Netconf and Restconf) in combination with standard YANG data models.
Improving Bridge Expansion and Contraction Installation Replacement Decision System Using Hybrid Chaotic Whale Optimization Algorithm
Authors: Xu Zian , Huang Minshui
Published: July 2021

Bridge expansion and contraction installation (BECI) has proved to be an essential component of the bridge structure due to its stability, comfort, and durability benefits. At present, traditional replacement technologies for modular type, comb plate type, and seamless type BECIs are widely applied worldwide. Nevertheless, it is unfortunate that the research conducted on decision-making (DM) approaches for the technical condition assessment and the optimal replacement plan selection of existing BECIs remain scarce, which results in the waste of resources and the increase in cost. Therefore, a BECI technical condition assessment approach, which contains specific on-site inspection regulations with both qualitative and quantitative descriptions, is proposed in this research, and a corresponding calculation program has been developed based on the MATLAB platform, which provides the basis for the necessity of replacement. Simultaneously, the hybrid chaotic whale optimization algorithm is designed and performed to improve and automate the process of optimal replacement plan selection under the assistance of the analytic hierarchy process (AHP), where both the achievement in consistency modification and the reservation of initial information are perused, and its superiority and effectiveness are verified via the comparative experimental analysis. The improved BECI replacement decision system is established, and the corresponding case study demonstrates that the proposed system in this research proves reasonable and feasible. The improved system can effectively assist bridge managers in making more informed operation and maintenance (O and M) decisions in actual engineering projects.
Visual Tracking Control of Cable-Driven Hyper-Redundant Snake-Like Manipulator
Authors: Zhou Qisong , Tang Jianzhong , Nie Yong , Chen Zheng , Qin Long
Published: July 2021

The cable-driven hyper-redundant snake-like manipulator (CHSM) inspired by the biomimetic structure of vertebrate muscles and tendons, which consists of numerous joint units connected adjacently driven by elastic materials with hyper-redundant DOF, performs flexible kinematic skills and competitive compound capability under complicated working circumstances. Nevertheless, the drawback of lacking the ability to perceive the environment to perform intelligently in complex scenarios leaves a lot to be improved, which is the original intention to introduce visual tracking feedback acting as an instructor. In this paper, a cable-driven snake-like robotic arm combined with a visual tracking technique is introduced. A visual tracking approach based on dual correlation filter is designed to guide the CHSM in detecting the target and tracing after its trajectory. Specifically, it contains an adaptive optimization for the scale variation of the tracking target via pyramid sampling. For the CHSM, an explicit kinematics model is derived from its specific geometry relationships and followed by a simplification for the inverse kinematics based on some assumption or limitation. A control scheme is brought up to combine the kinematics with visual tracking via the processing tracking errors. The experimental results with a practical prototype validate the availability of the proposed compound control method with the derived kinematics model.
A Study of Predictive Models for Early Outcomes of Post-Prostatectomy Incontinence: Machine Learning Approach vs. Logistic Regression Analysis Approach
Authors: Park Seongkeun , Byun Jieun
Published: July 2021

Background: Post-prostatectomy incontinence (PPI) is a major complication that can significantly decrease quality of life. Approximately 20% of patients experience consistent PPI as long as 1 year after radical prostatectomy (RP). This study develops a preoperative predictive model and compares its diagnostic performance with conventional tools. Methods: A total of 166 prostate cancer patients who underwent magnetic resonance imaging (MRI) and RP were evaluated. According to the date of the RP, patients were divided into a development cohort (n = 109) and a test cohort (n = 57). Patients were classified as PPI early-recovery or consistent on the basis of pad usage for incontinence at 3 months after RP. Uni- and multi-variable logistic regression analyses were performed to identify associates of PPI early recovery. Four well-known machine learning algorithms (k-nearest neighbor, decision tree, support-vector machine (SVM), and random forest) and a logistic regression model were used to build prediction models for recovery from PPI using preoperative clinical and imaging data. The performances of the prediction models were assessed internally and externally using sensitivity, specificity, accuracy, and area-under-the-curve values and estimated probabilities and the actual proportion of cases of recovery from PPI within 3 months were compared using a chi-squared test. Results: Clinical and imaging findings revealed that age (70.1 years old for the PPI early-recovery group vs. 72.8 years old for the PPI consistent group), membranous urethral length (MUL; 15.7 mm for the PPI early-recovery group vs. 13.9 mm for the PPI consistent group), and obturator internal muscle (18.2 mm for the PPI early-recovery group vs. 17.5 mm for the PPI consistent group) were significantly different between the PPI early-recovery and consistent groups (all p-values < 0.05). Multivariate analysis confirmed that age (odds ratio = 1.07, 95% confidence interval = 1.02–1.14, p-value = 0.007) and MUL (odds ratio = 0.87, 95% confidence interval = 0.80–0.95, p-value = 0.002) were significant independent factors for early recovery. The prediction model using machine learning algorithms showed superior diagnostic performance compared with conventional logistic regression (AUC = 0.59 ± 0.07), especially SVM (AUC = 0.65 ± 0.07). Moreover, all models showed good calibration between the estimated probability and actual observed proportion of cases of recovery from PPI within 3 months. Conclusions: Preoperative clinical data and anatomic features on preoperative MRI can be used to predict early recovery from PPI after RP, and machine learning algorithms provide greater diagnostic accuracy compared with conventional statistical approaches.
Experimental Investigation of Traditional Clay Brick and Lime Mortar Intended for Restoration of Cultural Heritage Sites
Authors: Lee Gayoon , Park Jun Hyoung , Pham Khoa V. A. , Lee Chan Hee , Lee Kihak
Published: July 2021

To properly restore masonry cultural heritage sites, the materials used for retrofitting can have a critical effect, and this requires standards for traditional Korean brick and lime mortar to be examined. This study experimentally investigated the material characteristics of Korean traditional bricks and two types of lime mortar (quicklime lumps and powdered hydrated lime) and the strength of masonry specimens made from those materials. Four different mixing ratios of lime, sand and white cement were considered as material parameters in this study. The experiment included uniaxial compressive testing and flexural testing to examine the mortars’ mechanical properties, and compression tests, triplet shear tests and diagonal compression tests for the masonry specimens. The results found that the strength of the masonry specimens was not necessarily associated with the mortar’s strength, but rather the cohesion between brick and mortar. In the material test, adding white cement had no noticeable effect on mortar strength. Meanwhile, in the masonry specimen, the effect of the added white cement was significant in terms of compressive and shear strength. This suggests that the bonding ratio between mortar and brick, which is an important factor influencing the behavior of bricks, was stronger with the addition of white cement. Furthermore, it was found that quicklime lumps had a lower strength than powdered hydrated lime. The test specimen with white cement added to powdered hydrated lime exhibited the greatest strength.
Continuous Control Set Predictive Current Control for Induction Machine
Authors: Varga Toni , Benšić Tin , Jerković Štil Vedrana , Barukčić Marinko
Published: July 2021

A speed tracking control method for induction machine is shown in this paper. The method consists of outer speed control loop and inner current control loop. Model predictive current control method without the need for calculation of the weighing factors is utilized for the inner control loop, which generates a continuous set of voltage reference values that can be modulated and applied by the inverter to the induction machine. Interesting parallels are drawn between the developed method and state feedback principles that helped with the analysis of the stability and controllability. Simple speed and rotor flux estimator is implemented that helps achieve sensorless control. Simulation is conducted and the method shows great performance for speed tracking in a steady state, and during transients as well. Additionally, compared to the finite control set predictive current control, it shows less harmonic content in the generated torque on the rotor shaft.
Photonic Integrated Reconfigurable Linear Processors as Neural Network Accelerators
Authors: De Marinis Lorenzo , Cococcioni Marco , Liboiron-Ladouceur Odile , Contestabile Giampiero , Castoldi Piero , Andriolli Nicola
Published: July 2021

Reconfigurable linear optical processors can be used to perform linear transformations and are instrumental in effectively computing matrix–vector multiplications required in each neural network layer. In this paper, we characterize and compare two thermally tuned photonic integrated processors realized in silicon-on-insulator and silicon nitride platforms suited for extracting feature maps in convolutional neural networks. The reduction in bit resolution when crossing the processor is mainly due to optical losses, in the range 2.3–3.3 for the silicon-on-insulator chip and in the range 1.3–2.4 for the silicon nitride chip. However, the lower extinction ratio of Mach–Zehnder elements in the latter platform limits their expressivity (i.e., the capacity to implement any transformation) to 75%, compared to 97% of the former. Finally, the silicon-on-insulator processor outperforms the silicon nitride one in terms of footprint and energy efficiency.
Multiparametric Analysis of a Gravity Retaining Wall
Authors: Varga Rok , Žlender Bojan , Jelušič Primož
Published: July 2021

The design of a gravity retaining wall should be simple to construct, quick to build and the best economic solution to a problem. This can be achieved by using advanced optimization methods. Since geotechnical engineers are not always able to determine the exact soil properties and other project data, an optimal design of a gravity retaining wall should also be determined for a wide range of input parameters. Therefore, a multiparametric analysis of an optimal designed gravity retaining wall was carried out. Optimum designs of gravity retaining walls were obtained for 567 combinations of different design parameters. Diagrams were developed to help engineers determine the optimum section of the wall, based on construction costs. An exhaustive search was carried out within the available parameters (project data). The parameters were ranked according to which had the most influence on the optimum cost of the gravity retaining wall and the utilization of multiple constraints. The most important parameter for the optimal cost of a gravity retaining wall is the height of the retained ground, followed by the shear angle of the soil, the soil–wall interaction coefficient, the slope angle and the variable surcharge load. The shear angle of the soil is most relevant to the bearing capacity and eccentricity condition, while the soil–wall interaction coefficient is most relevant to the sliding condition. Since European countries apply different load, material and resistance safety factors, the optimization model was developed in a general form, where different design approaches and unit prices could be applied. The case study provides an improved optimization model for selecting the optimal design of gravity walls, for engineers.
Fast Low-Precision Computer-Generated Holography on GPU
Authors: Blinder David , Schelkens Peter
Published: July 2021

Computer-generated holography (CGH) is a notoriously difficult computation problem, simulating numerical diffraction, where every scene point can affect every hologram pixel. To tackle this challenge, specialized software instructions and hardware solutions are developed to significantly reduce calculation time and power consumption. In this work, we propose a novel algorithm for high-performance point-based CGH, leveraging fixed-point integer representations, the separability of the Fresnel transform and using new look-up table free cosine representation. We report up to a 3-fold speed up over an optimized floating-point GPU implementation, as well as a 15 dB increase in quality over a state-of-the-art FPGA-based fixed-point integer solution.
Diversity and Seasonal Dynamics of Airborne Fungi in Nerja Cave, Spain
Authors: Jurado Valme , Del Rosal Yolanda , Liñan Cristina , Martin-Pozas Tamara , Gonzalez-Pimentel Jose Luis , Saiz-Jimenez Cesareo
Published: July 2021

Nerja Cave, Southern Spain, was revealed as an important biodiversity reservoir from which several novel species of Aspergillus were described. We carried out an aerobiological study in Nerja Cave to assess the origin of airborne fungi. This study quantified the fungi present in the air of ten representative halls covering the three sectors comprising the cave: Touristic Galleries, High Galleries, and New Galleries. Microclimatological monitoring allowed us to understand the dynamic of airborne fungi in two seasons of the year (winter and summer), corresponding to the strongest and the lowest cave ventilation, and to validate the influence that the transport of airborne fungi from outside may have on the cave itself. The data show that cold air enters in winter, as confirmed by the abundant presence of Aspergillus and Penicillium spores inside and outside the cave. In summer, the abundance of some fungi in the air of Nerja Cave, which are not detected outside, indicates a stagnation or low ventilation, and therefore, the concentration of fungal spores is maxima. The high occurrence of Cladosporium outside the cave and the scarce abundance inside support the cave stagnation in this season.
Application Study on Fiber Optic Monitoring and Identification of CRTS-II-Slab Ballastless Track Debonding on Viaduct
Authors: Guo Gaoran , Wang Junfang , Du Bowen , Du Yanliang
Published: July 2021

China Railway Track System (CRTS)-II-slab ballastless track is a new type of track structure, and its interlayer connection state is considerably important for the operation safety and ride comfort of high-speed trains. However, the location and multiple influencing factors of interlayer debonding lead to difficulties in monitoring and identification. Here, the research on the design and application of a monitoring scheme that facilitates interlayer debonding detection of ballastless track and an effective indicator for debonding identification and assessment is proposed. The results show that on-site monitoring can effectively capture the vibration signals caused by train vibration and interlayer debonding. The features of the data acquired in the situations with and without interlayer debonding are compared after instantaneous baseline validation. Some significant features capable of obviously differentiating a debonding state from the normal state are identified. Furthermore, a new indicator, combining multiple debonding-sensitive features by similarity-based weights normalizing the initial difference between mutual instantaneous baselines, is developed to support rational and comprehensive assessment quantitatively. The contribution of this study includes the development and application of an interlay-debonding monitoring scheme, the establishment of an effective-feature pool, and the proposal of the similarity-based indicator, thereby laying a good foundation for debonding identification of ballastless track.
Energy Consumption Evaluation of Active Tillage Machines Using Dynamic Modelling
Authors: Forgó Zoltán , Tolvaly-Roșca Ferenc , Pásztor Judit , Kovari Attila
Published: July 2021

Soil tillage is a very energy-intensive operation. A general expectation is to reduce energy consumption and reduce soil compaction with as few turns and interventions as possible. Thus, more and more attention is being paid to the use of active tillage machines. The aim of the present work is to test a new approach to optimize PTO-driven tilling machine operations regarding energy consumption. A real, active tillage machine, the MSS-140 type spading machine, was investigated in the Matlab® Simscape™ environment. The solid model of the spading machine was built using actual dimensions. The work done by a single spade is broken down into elementary processes. The acting forces on the implement, in each elementary process for different advancing speeds, were modelled and calculated. The model is suitable for illustrating the dynamics of loads and for calculating the mechanical work. The model was also tested in comparison with real fuel consumption. The consumed fuel quantity was measured and the energy requirement for the model calculated at three advancing speeds. A comparison between the measured and calculated energy consumption values was made: the calculated results are similar to the measured values; the mean difference is 9.91%, with a standard deviation 3.3%.