bluuebunny/crossref_metadata_2025 huggingface dataset:
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Authors: López-Serrano Sergi , Galofré-Milà Nuria , Costa-Hurtado Mar , Pérez-de-Rozas Ana M. , Aragon Virginia
Published: January 2020

Background

Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine.

Results

In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains.

Conclusions

In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
Mesenchymal stem cells derived from iPSCs expressing interleukin-24 inhibit the growth of melanoma in the tumor-bearing mouse model
Authors: Wu Zheng , Liu Wei , Wang Zujia , Zeng Baitao , Peng Guangnan , Niu Hongyan , Chen Linlin , Liu Cong , Hu Qian , Zhang Yuxuan , Pan Mengmeng , Wu Lingqian , Liu Mujun , Liu Xionghao , Liang Desheng
Published: January 2020

Background

Interleukin-24 (IL-24) is a therapeutic gene for melanoma, which can induce melanoma cell apoptosis. Mesenchymal stem cells (MSCs) show promise as a carrier to delivery anti-cancer factors to tumor tissues. Induced pluripotent stem cells (iPSCs) are an alternative source of mesenchymal stem cells (MSCs). We previously developed a novel non-viral gene targeting vector to target IL-24 to human iPSCs. This study aims to investigate whether MSCs derived from the iPSCs with the site-specific integration of IL-24 can inhibit the growth of melanoma in a tumor-bearing mouse model via retro-orbital injection.

Methods

IL-24-iPSCs were differentiated into IL-24-iMSCs in vitro, of which cellular properties and potential of differentiation were characterized. The expression of IL-24 in the IL-24-iMSCs was measured by qRT-PCR, Western Blotting, and ELISA analysis. IL-24-iMSCs were transplanted into the melanoma-bearing mice by retro-orbital intravenous injection. The inhibitory effect of IL-24-iMSCs on the melanoma cells was investigated in a co-culture system and tumor-bearing mice. The molecular mechanisms underlying IL-24-iMSCs in exerting anti-tumor effect were also explored.

Results

iPSCs-derived iMSCs have the typical profile of cell surface markers of MSCs and have the ability to differentiate into osteoblasts, adipocytes, and chondroblasts. The expression level of IL-24 in IL-24-iMSCs reached 95.39 ng/106 cells/24 h, which is significantly higher than that in iMSCs, inducing melanoma cells apoptosis more effectively in vitro compared with iMSCs. IL-24-iMSCs exerted a significant inhibitory effect on the growth of melanoma in subcutaneous mouse models, in which the migration of IL-24-iMSCs to tumor tissue was confirmed. Additionally, increased expression of Bax and Cleaved caspase-3 and down-regulation of Bcl-2 were observed in the mice treated with IL-24-iMSCs.

Conclusion

MSCs derived from iPSCs with the integration of IL-24 at rDNA locus can inhibit the growth of melanoma in tumor-bearing mouse models when administrated via retro-orbital injection.
Correction to: Association of PM2.5 concentration with health center outpatient visits for respiratory diseases of children under 5 years old in Lima, Peru
Authors: Davila Cordova Jennifer Estefanía , Aguirre Vilma Tapia , Apestegui Vanessa Vasquez , Ibarguen Luis Ordoñez , Vu Bryan N. , Steenland Kyle , Gonzales Gustavo F.
Published: January 2020

The original version of this article [1], published on 15 January 2020, contained incorrect name of the co- author. In this Correction the affected part of the article is shown.
Biodegradable polymer nanocomposites for ligament/tendon tissue engineering
Authors: Silva Magda , Ferreira Fernando N. , Alves Natália M. , Paiva Maria C.
Published: January 2020

Ligaments and tendons are fibrous tissues with poor vascularity and limited regeneration capacity. Currently, a ligament/tendon injury often require a surgical procedure using auto- or allografts that present some limitations. These inadequacies combined with the significant economic and health impact have prompted the development of tissue engineering approaches. Several natural and synthetic biodegradable polymers as well as composites, blends and hybrids based on such materials have been used to produce tendon and ligament scaffolds. Given the complex structure of native tissues, the production of fiber-based scaffolds has been the preferred option for tendon/ligament tissue engineering. Electrospinning and several textile methods such as twisting, braiding and knitting have been used to produce these scaffolds. This review focuses on the developments achieved in the preparation of tendon/ligament scaffolds based on different biodegradable polymers. Several examples are overviewed and their processing methodologies, as well as their biological and mechanical performances, are discussed.
Analysis of viral diversity in stool samples from infants and children with acute gastroenteritis in Kuwait using Metagenomics approach
Authors: Mohammad Hawraa Adel , Madi Nada Mohammed , Al-Nakib Widad
Published: January 2020

Background

Current molecular target-dependent methods are used to detect only known viruses. However, metagenomics based on next-generation sequencing (NGS) technique is a target-independent assay that enables simultaneous detection and genomic characterisation of all microorganisms present in a sample. In this study, we aimed to develop a metagenomics approach using NGS to identify and characterise viruses in stool samples from infants and children with Acute Gastroenteritis (AGE) in Kuwait.

Methods

We have investigated 84 stool samples from infants and children aged one month to ten years old with signs and symptoms of gastroenteritis who attended Mubarak Al-Kabeer and Al-Amiri hospitals in Kuwait from January to December 2017. A metagenomics approach using NGS to characterise viruses in clinical samples was used. Also, the commercial Real-Time PCR assay was used to detect viruses causing gastroenteritis.

Results

Metagenomics analysis revealed an average of 280,768 reads in which 5% of the reads were derived from viruses. The analysis of viral sequences verified that single infection of human adenovirus was the leading cause of gastroenteritis among infants and children, which was detected in 23.2% of the patients, followed by a mixed infection of human adenovirus and other viruses, which was detected in 20.9% of patients. Also, the newly discovered viruses known to cause gastroenteritis were detected, such as astrovirus MLB2, primate bocaparvovirus-1, Aichivirus A, cardiovirus, parechovirus A, astrovirus VA4, cosavirus-F, and bufavirus-3. Our results showed 71% agreement (k = 0.445, P = 0.000) between multiplex Real-Time PCR, which is used as a routine diagnostic test and metagenomics approach in the detection of viruses causing gastroenteritis in clinical samples.

Conclusion

Despite the difficulties in sample preparation and analysis process, we showed that metagenomics approach is a powerful and promising tool for the detection and characterisation of different viruses in clinical samples.
Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro
Authors: Nishihara Hideaki , Soldati Sasha , Mossu Adrien , Rosito Maria , Rudolph Henriette , Muller William A. , Latorre Daniela , Sallusto Federica , Sospedra Mireia , Martin Roland , Ishikawa Hiroshi , Tenenbaum Tobias , Schroten Horst , Gosselet Fabien , Engelhardt Britta
Published: February 2020

Background

The brain barriers establish compartments in the central nervous system (CNS) that significantly differ in their communication with the peripheral immune system. In this function they strictly control T-cell entry into the CNS. T cells can reach the CNS by either crossing the endothelial blood–brain barrier (BBB) or the epithelial blood-cerebrospinal fluid barrier (BCSFB) of the choroid plexus (ChP).

Objective

Analysis of the cellular and molecular mechanisms involved in the migration of different human CD4+ T-cell subsets across the BBB versus the BCSFB.

Methods

Human in vitro models of the BBB and BCSFB were employed to study the migration of circulating and CNS-entry experienced CD4+ T helper cell subsets (Th1, Th1*, Th2, Th17) across the BBB and BCSFB under inflammatory and non-inflammatory conditions in vitro.

Results

While under non-inflammatory conditions Th1* and Th1 cells preferentially crossed the BBB, under inflammatory conditions the migration rate of all Th subsets across the BBB was comparable. The migration of all Th subsets across the BCSFB from the same donor was 10- to 20-fold lower when compared to their migration across the BBB. Interestingly, Th17 cells preferentially crossed the BCSFB under both, non-inflamed and inflamed conditions. Barrier-crossing experienced Th cells sorted from CSF of MS patients showed migratory characteristics indistinguishable from those of circulating Th cells of healthy donors. All Th cell subsets could additionally cross the BCSFB from the CSF to ChP stroma side. T-cell migration across the BCSFB involved epithelial ICAM-1 irrespective of the direction of migration.

Conclusions

Our observations underscore that different Th subsets may use different anatomical routes to enter the CNS during immune surveillance versus neuroinflammation with the BCSFB establishing a tighter barrier for T-cell entry into the CNS compared to the BBB. In addition, CNS-entry experienced Th cell subsets isolated from the CSF of MS patients do not show an increased ability to cross the brain barriers when compared to circulating Th cell subsets from healthy donors underscoring the active role of the brain barriers in controlling T-cell entry into the CNS. Also we identify ICAM-1 to mediate T cell migration across the BCSFB.
So, what is chiropractic? Summary and reflections on a series of papers in Chiropractic and Manual Therapies
Authors: Hartvigsen Jan , French Simon D.
Published: January 2020

This commentary brings the 2017–2019 thematic series What is Chiropractic? to a close. The 18 papers published in the series contribute to a better understanding of what chiropractic is, where chiropractors practice and function, who seeks their care, what chiropractors do, and how they interact with other healthcare professionals. Several papers in the series highlighted deeply rooted disagreements within chiropractic about fundamental issues pertaining to ideology, acceptance of scientific evidence as the basis for clinical practice and the future of chiropractic. If the chiropractic profession is to remain relevant in today’s evidence-based healthcare environment, there is an urgent for the profession to undertake further research to describe what chiropractic is, what chiropractors do, and provide evidence for the value of these activities to patients and healthcare decision makers.
Host proteome linked to HPV E7-mediated specific gene hypermethylation in cancer pathways
Authors: Na Rangsee Nopphamon , Yanatatsaneejit Pattamawadee , Pisitkun Trairak , Somparn Poorichaya , Jintaridth Pornrutsami , Topanurak Supachai
Published: February 2020

Background

Human papillomavirus (HPV) infection causes around 90% of cervical cancer cases, and cervical cancer is a leading cause of female mortality worldwide. HPV-derived oncoprotein E7 participates in cervical carcinogenesis by inducing aberrant host DNA methylation. However, the targeting specificity of E7 methylation of host genes is not fully understood but is important in the down-regulation of crucial proteins of the hallmark cancer pathways. In this study, we aim to link E7-driven aberrations in the host proteome to corresponding gene promoter hypermethylation events in the hope of providing novel therapeutic targets and biomarkers to indicate the progression of cervical cancer.

Methods

HEK293 cells were transfected with pcDNA3.1-E7 plasmid and empty vector and subjected to mass spectrometry-based proteomic analysis. Down-regulated proteins (where relative abundance was determined significant by paired T-test) relevant to cancer pathways were selected as gene candidates for mRNA transcript abundance measurement by qPCR and expression compared with that in SiHa cells (HPV type 16 positive). Methylation Specific PCR was used to determine promoter hypermethylation in genes downregulated in both SiHa and transfected HEK293 cell lines. The FunRich and STRING databases were used for identification of potential regulatory transcription factors and the proteins interacting with transcription factor gene candidates, respectively.

Results

Approximately 400 proteins totally were identified in proteomics analysis. The transcripts of six genes involved in the host immune response and cell proliferation (PTMS, C1QBP, BCAP31, CDKN2A, ZMYM6 and HIST1H1D) were down-regulated, corresponding to proteomic results. Methylation assays showed four gene promoters (PTMS, C1QBP, BCAP31 and CDKN2A) were hypermethylated with 61, 55.5, 70 and 78% increased methylation, respectively. Those four genes can be regulated by the GA-binding protein alpha chain, specificity protein 1 and ETS-like protein-1 transcription factors, as identified from FunRich database predictions.

Conclusions

HPV E7 altered the HEK293 proteome, particularly with respect to proteins involved in cell proliferation and host immunity. Down-regulation of these proteins appears to be partly mediated via host DNA methylation. E7 possibly complexes with the transcription factors of its targeting genes and DNMT1, allowing methylation of specific target gene promoters.
How POCUS picks up a rare mimickers of acute appendicitis in the emergency department
Authors: Al Sheikh Shihab , Al Ali Mariam , Kiran Dhanya Sochel , Al Tabatabai Mohsen
Published: February 2020

Background

Appendicitis is the most common surgical abdominal emergency. Punctual diagnosis and rapid operative treatment of acute appendicitis are critically important, as it reduces the risk of complications, associated with greater morbidity and cost of care. The clinical diagnosis of acute appendicitis can be difficult and confusing. Due to some typical presentation and mimic, several abdominal conditions are left undiagnosed. POCUS has comparatively acceptable sensitivity and high specificity for diagnosing acute appendicitis, and early practice POCUS has a standalone test to rule out acute appendicitis.

Case presentation

A 43-year-old man presented with a 3-day history of abdominal pain rated 5/10 in intensity which had localized to the right iliac fossa by the time he attended our emergency. He described the acute pain as sharp in nature, colicky without the presence of any radiation. The pain was associated with nausea, but there was no vomiting. On clinical examination, the patient was stable at first, had a normal temperature with tenderness and guarding the right iliac fossa under nearby normal laboratory investigation.

Conclusion

The importance of POCUS in scanning right iliac fossa for patients present with signs and symptoms that are mimicking acute appendicitis for diagnosing a rare pathology and avoiding the risk of ionizing radiation hazards and unnecessary surgical intervention.
Enhancement of the serum chloride concentration by administration of sodium–glucose cotransporter-2 inhibitor and its mechanisms and clinical significance in type 2 diabetic patients: a pilot study
Authors: Kataoka Hajime , Yoshida Yuichi
Published: February 2020

Background

Chloride is a key electrolyte that regulates the body fluid distribution. Accordingly, manipulating chloride kinetics by selecting a suitable diuretic could be an attractive strategy for correcting body fluid dysregulation. Therefore, this study examined the effects and contributing factors of a sodium–glucose cotransporter-2 inhibitor (SGLT2i) on the serum chloride concentration in type 2 diabetic (T2DM) patients without heart failure (HF).

Methods

This study was a retrospective single-center observational study that enrolled 10 T2DM/non-HF outpatients for whom the SGLT2i empagliflozin (daily oral dose of 10 mg) was prescribed. Among these 10 patients, 6 underwent detailed clinical testing that included hormonal and metabolic blood tests.

Results

Empagliflozin treatment for 1–2 months decreased body weight (− 2.69 ± 1.9 kg; p = 0.002) and HbA1c (− 0.88 ± 0.55%; p = 0.0007). The hemoglobin (+ 0.27 ± 0.36 g/dL; p = 0.04) and hematocrit (+ 1.34 ± 1.38%; p = 0.014) values increased, but the serum creatinine concentration remained unchanged. The serum chloride concentration increased from 104 ± 3.23 to 106 ± 2.80 mEq/L (p = 0.004), but the sodium and potassium concentrations did not change. The spot urinary sodium concentration decreased from 159 ± 43 to 98 ± 35 mEq/L (p < 0.02) and the spot urinary chloride tended to decrease (from 162 ± 59 to 104 ± 36 mEq/L, p < 0.08). Both renin and aldosterone tended to be activated (5/6, 83%). The strong organic acid metabolite concentrations of serum acetoacetate (from 42 ± 25 to 100 ± 45 μmol/L, p < 0.02) and total ketone bodies (from 112 ± 64 to 300 ± 177 μmol/L, p < 0.04) increased, but the actual HCO3−concentration decreased (from 27 ± 2.5 to 24 ± 1.6 mEq/L, p < 0.008).

Conclusions

The present study demonstrated that SGLT2i enhances the serum chloride concentration in T2DM patients and suggests that the effect is mediated by the possible following mechanisms: (1) enhanced reabsorption of urinary chloride by aldosterone activation due to blood pressure lowering and blood vessel contraction effects, (2) reciprocal increase in the serum chloride concentration by reducing the serum HCO3−concentration via a buffering effect of strong organic acid metabolites, and (3) reduced NaHCO3reabsorption and concurrently enhanced chloride reabsorption in the urinary tubules by inhibiting Na+–H+exchanger 3 in the renal proximal tubules. Thus, the diuretic SGLT2i induces excessive extravascular fluid to drain into the vascular space by the enhanced vascular “tonicity” caused by the elevated serum chloride concentration.
Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration
Authors: Parate Dinesh , Kadir Nurul Dinah , Celik Cenk , Lee Eng Hin , Hui James H. P. , Franco-Obregón Alfredo , Yang Zheng
Published: February 2020

Background

The mesenchymal stem cell (MSC) secretome, via the combined actions of its plethora of biologically active factors, is capable of orchestrating the regenerative responses of numerous tissues by both eliciting and amplifying biological responses within recipient cells. MSCs are “environmentally responsive” to local micro-environmental cues and biophysical perturbations, influencing their differentiation as well as secretion of bioactive factors. We have previously shown that exposures of MSCs to pulsed electromagnetic fields (PEMFs) enhanced MSC chondrogenesis. Here, we investigate the influence of PEMF exposure over the paracrine activity of MSCs and its significance to cartilage regeneration.

Methods

Conditioned medium (CM) was generated from MSCs subjected to either 3D or 2D culturing platforms, with or without PEMF exposure. The paracrine effects of CM over chondrocytes and MSC chondrogenesis, migration and proliferation, as well as the inflammatory status and induced apoptosis in chondrocytes and MSCs was assessed.

Results

We show that benefits of magnetic field stimulation over MSC-derived chondrogenesis can be partly ascribed to its ability to modulate the MSC secretome. MSCs cultured on either 2D or 3D platforms displayed distinct magnetic sensitivities, whereby MSCs grown in 2D or 3D platforms responded most favorably to PEMF exposure at 2 mT and 3 mT amplitudes, respectively. Ten minutes of PEMF exposure was sufficient to substantially augment the chondrogenic potential of MSC-derived CM generated from either platform. Furthermore, PEMF-induced CM was capable of enhancing the migration of chondrocytes and MSCs as well as mitigating cellular inflammation and apoptosis.

Conclusions

The findings reported here demonstrate that PEMF stimulation is capable of modulating the paracrine function of MSCs for the enhancement and re-establishment of cartilage regeneration in states of cellular stress. The PEMF-induced modulation of the MSC-derived paracrine function for directed biological responses in recipient cells or tissues has broad clinical and practical ramifications with high translational value across numerous clinical applications.
The hyperbolic model for edge and texture detection in the primary visual cortex
Authors: Chossat Pascal
Published: January 2020

The modeling of neural fields in the visual cortex involves geometrical structures which describe in mathematical formalism the functional architecture of this cortical area. The case of contour detection and orientation tuning has been extensively studied and has become a paradigm for the mathematical analysis of image processing by the brain. Ten years ago an attempt was made to extend these models by replacing orientation (an angle) with a second-order tensor built from the gradient of the image intensity, and it was named the structure tensor. This assumption does not follow from biological observations (experimental evidence is still lacking) but from the idea that the effectiveness of texture processing with the structure tensor in computer vision may well be exploited by the brain itself. The drawback is that in this case the geometry is not Euclidean but hyperbolic instead, which complicates the analysis substantially. The purpose of this review is to present the methodology that was developed in a series of papers to investigate this quite unusual problem, specifically from the point of view of tuning and pattern formation. These methods, which rely on bifurcation theory with symmetry in the hyperbolic context, might be of interest for the modeling of other features such as color vision or other brain functions.
Variations in association of nasal microbiota with virulent and non-virulent strains of Glaesserella (Haemophilus) parasuis in weaning piglets
Authors: Mahmmod Yasser S. , Correa-Fiz Florencia , Aragon Virginia
Published: February 2020

Glaesserella (formerly Haemophilus) parasuis causes Glässer’s disease, which results in high economic loss in the swine industry. To understand the polymicrobial interactions of G. parasuis and the nasal microbiota, the statistical association patterns of nasal colonizing bacteria with virulent and non-virulent strains of G. parasuis were studied accounting for the farm management practices as potential risk factors for the occurrence of Glässer’s disease. The nasal microbiota from 51 weaned-piglets from four farms with Glässer’s disease and three farms with no respiratory diseases was previously characterized and included in this study. The presence of virulent and/or non-virulent G. parasuis strains in the nasal cavities was determined in order to establish the potential association with other members of the nasal microbiota. Multivariate logistic and linear regression models were performed among the various members of nasal microbiota and G. parasuis. The multi-site production system and disease presence in the farm were both significantly associated with the presence of G. parasuis virulent strains in the nose of the piglets. Differential bacterial associations were observed with virulent or non-virulent G. parasuis. Chitinophagaceae, Corynebacteriaceae and Corynebacterium were positively associated with the virulent G. parasuis strains, while Enterobacteriaceae, Peptostreptococcaceae, Clostridium XI, and Escherichia/Shigella were negatively associated with virulent G. parasuis. On the other hand, Flavobacteriaceae, Planobacterium, and Phascolarctobacterium were positively associated with the non-virulent G. parasuis strains, while Rikenellaceae, Enterococcaceae, Odoribacter, and Corynebacterium were negatively associated with non-virulent G. parasuis. In conclusion, the nasal microbiota communities showed variations in the association with the G. parasuis strains type.
Synergistic renoprotective effects of sesame oil and erythropoietin on ischemic kidney injury after renal transplantation
Authors: Ye Liyu , Xiao Fang , Xie Jijun , Feng Lingling , Tang Zhangjun , Chen E. , Chen Chuchu , Xu Bowen , Deng Ronghai
Published: February 2020

In this study, we evaluated the combined therapeutic efficacy of erythropoietin (a hematopoietic hormone produced by the fetal liver and kidney in response to inflammation and apoptosis) and sesame oil (from Sesamum indicum L.) on ischemic kidney injury following kidney transplantation in a rat model. Rats were assigned to the following groups: sham, control, 1000 U/kg erythropoietin, 1 mL/kg sesame oil, 1000 U/kg erythropoietin + 1 mL/kg sesame oil, and positive control. We measured the levels of blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), lipid peroxidation, reactive oxygen species (ROS), reduced glutathione (GSH), antioxidant enzymes, and proinflammatory markers and performed renal histopathological evaluation. The combined erythropoietin and sesame oil treatment significantly reduced BUN, ALT, creatinine, lipid peroxidation, ROS, and proinflammatory markers and GSH and antioxidant enzyme levels. Histopathological examination showed that the combined erythropoietin and sesame oil treatment significantly reduced necrosis. Therefore, combined treatment of sesame oil and erythropoietin may represent an effective therapeutic approach against ischemic kidney injury after kidney transplantation.
The accumulation of rare-earth yttrium ions by Penicillium sp. ZD28
Authors: Wang Weiying , Xu Chenglong , Jin Yiqiao , Zhang Zhibin , Yan Riming , Zhu Du
Published: February 2020

To obtained fungal resources with excellent tolerance and accumulation capacity to rare earth yttrium ions (Y3+), rare earth ore samples were collected and used for microbial screening. A fungus hyper-resistant to Y3+ was obtained and the effects of the fungus in three physiological states (growth process, mycelial pellets with physiological activity and the fungus powder after being ground) on the Y3+ accumulation were investigated. The Y3+ resistant fungus was identified as Penicillium sp. ZD28, and its mycelium pellets (about 1 mm in diameter) showed poor ability to accumulate Y3+ with an adsorption capacity of less than 81 μmol/g. However, the fungus was able to remove 99% of Y3+ during the growth process, at an initial concentration of less than 600 μM. Bioaccumulation of Y was observed on the cell surface of the ZD28 strain by elemental mapping using scanning electron microscopy-energy dispersive X-ray spectroscopy. The adsorbent (the dry fungal powder) had a remarkable adsorption property for Y3+ that was greater than 455 μmol/g in conditions of 465 μM < [Y3+] < 6382 μM. Penicillium sp. ZD28 has major potential applications in the accumulation of yttrium group rare earth ions. This research has formed a theoretical foundation for the application of this biological method to extract rare earth ions in the mining and smelting of yttrium group rare earth elements.
Control of mesenchymal stem cell biology by histone modifications
Authors: Ren Jianhan , Huang Delan , Li Runze , Wang Weicai , Zhou Chen
Published: February 2020

Mesenchymal stem cells (MSCs) are considered the most promising seed cells for regenerative medicine because of their considerable therapeutic properties and accessibility. Fine-tuning of cell biological processes, including differentiation and senescence, is essential for achievement of the expected regenerative efficacy. Researchers have recently made great advances in understanding the spatiotemporal gene expression dynamics that occur during osteogenic, adipogenic and chondrogenic differentiation of MSCs and the intrinsic and environmental factors that affect these processes. In this context, histone modifications have been intensively studied in recent years and have already been indicated to play significant and universal roles in MSC fate determination and differentiation. In this review, we summarize recent discoveries regarding the effects of histone modifications on MSC biology. Moreover, we also provide our insights and perspectives for future applications.
Web intrusion detection system combined with feature analysis and SVM optimization
Authors: Liu Chao , Yang Jing , Wu Jinqiu
Published: February 2020

The current network traffic is large, and the network attacks have multiple types. Therefore, anomaly detection model combined with machine learning is developing rapidly. Frequent occurrences of Web Application Firewall (WAF) bypass attacks and the redundancy of the data characteristics in Hypertext Transfer Protocol (HTTP) protocol make it difficult to extract data characteristics. In this paper, an integrated web intrusion detection system combined with feature analysis and support vector machine (SVM) optimization is proposed. By using expert’s knowledge, the characteristics of the common Web attacks are analyzed. The related data characteristics are selected by the analysis of the HTTP protocol. In the classification learning, the mature and robust support vector machine algorithm is utilized and the grid search method is used for the parameter optimization. Consequently, a better detection capability on Web attacks can be obtained. By using the HTTP DATASET CSIC 2010 data set, experiments have been carried out to compare the detection capability of different kernel functions. The results show that the proposed system performs good in the detection capability and can detect the WAF bypass attacks effectively.
Channel estimation based on the PSS-MUSIC for millimeter-wave MIMO systems equipped with co-prime arrays
Authors: Li Shufeng , Cao Guangjing , Jin Libiao , Wu Hongda
Published: February 2020

The millimeter-wave channel estimation problem can be solved by estimating the path directions and the path gains. The previous schemes are nearly all based on the uniform linear arrays (ULAs). However, compared with the ULAs, the co-prime array can use fewer array elements to realize larger array aperture, which is beneficial to improve the performance of arrival direction estimation contributing to the estimation performance of angle of arrival (AOA) and angle of departures (AOD). Encouraged by the property of co-prime arrays, a novel channel estimation scheme is proposed for two-dimensional (2-d) co-prime arrays. For each path direction, multiple peaks are generated in the spatial spectrum of each subarray and by selecting over any limited sector to search for an arbitrary peak, which can recover the rest peaks. Then by comparing the peaks of the two subarrays, the common peaks are the correct path direction. Compared with the total-spectrum search (TSS) method, this method effectively reduces the complexity. Simulation results show that the proposed algorithm can reduce the computational complexity and maintain accurate channel estimation.
Prognostic-factors for neurodegeneration in chronic moderate-to-severe traumatic brain injury: a systematic review protocol
Authors: Sharma Bhanu , Changoor Alana , Monteiro Leanne , Colella Brenda , Green Robin
Published: February 2020

Background

Traumatic brain injury (TBI) is a leading cause of death and disability. Recently, a paradigm shift in our understanding of moderate-to-severe TBI has led to its reconceptualization as a progressive neurodegenerative disorder. Widespread progressive atrophy is observed in the months and years post-injury, long after the acute effects of the injury have resolved. Some studies have begun to examine prognostic demographic, injury-related, and post-injury risk factors that contribute to these declines. A synthesis of this information, and in particular, an increased understanding of post-injury factors that may be modifiable, would improve our ability to design interventions to reduce neurodegeneration in moderate-to-severe TBI. This systematic review aims to identify prognostic factors for neural deterioration in moderate-to-severe TBI, and thereby inform future intervention research in this population.

Methods

This review protocol was informed by and conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P) guidelines. Search strategies (designed to identify literature on prognostic factors of neurodegeneration in adults with moderate-to-severe TBI) optimized for MEDLINE, EMBASE PsychINFO, CINAHL, SportDiscus, and Cochrane Central Register of Controlled Trials will be developed with the assistance of a health sciences librarian. Retrieved studies will be screened by two team members. Studies must report on longitudinal neuroimaging (i.e., two or more scans in the same cohort) or neuroimaging in a cross-sectional study and potential prognostic factors for neurodegeneration, such as demographics (e.g., gender, age, education), injury (e.g., severity, etiology), or post-injury characteristics (e.g., type and length of therapy, activity level, mood).

Discussion

By identifying prognostic factors for neurodegeneration, this systematic review can help inform injury management, as well as intervention research designed to offset the effects of modifiable prognostic factors, such as low levels of cognitive or physical activity. In turn, this systematic review can increase our understanding of how to improve outcome following moderate-to-severe TBI.

Systematic review registration

PROSPERO CRD42019122389
A spectral element Crank–Nicolson model to the 2D unsteady conduction–convection problems about vorticity and stream functions
Authors: Teng Fei , Zhu Taiying , Luo Zhendong
Published: January 2020

In this study, a time semi-discretized Crank–Nicolson (CN) scheme of the two-dimensional (2D) unsteady conduction–convection problems for vorticity and stream functions is first built together with showing the existence and stability along with error estimates to the semi-discretized CN solutions. Afterwards, a fully discretized spectral element CN (SECN) model of the 2D unsteady conduction–convection problems as regards the vorticity and stream functions is set up together with showing the proof of the existence and stability along with error estimates of the SECN solution. Lastly, a set of numerical experiments are offered for checking the correctness of the theoretical conclusions.
Existence of rotating-periodic solutions for nonlinear second order vector differential equations
Authors: Zhang Jin , Yang Xue
Published: January 2020

In this paper, we establish two existence theorems of rotating-periodic solutions for nonlinear second order vector differential equations via the Leray–Schauder degree theory and the lower and upper solutions method. The concept “rotating-periodicity” is a kind of symmetry, which is a general version of periodicity, anti-periodicity, harmonic-periodicity, and it is also a special kind of quasi-periodicity. We also include several examples to illustrate the validity and applicability of our results.
The existence of solutions for perturbed fractional differential equations with impulses via Morse theory
Authors: Li Peiluan , Xu Liang , Wang Hui , Wang Yumeng
Published: January 2020

In the present paper, the existence of nontrivial solutions of impulsive fractional differential equations with Dirichlet boundary conditions is studied. We apply Morse theory coupled with local linking arguments to solve the topic, and we prove the existence of at least one nontrivial solution for the impulsive fractional differential equations.
Ground states for a coupled Schrödinger system with general nonlinearities
Authors: Duan Xueliang , Wei Gongming , Yang Haitao
Published: January 2020

We study a coupled Schrödinger system with general nonlinearities. By using variational methods, we prove the existence and asymptotic behaviour of ground state solution for the system with periodic couplings. Moreover, we prove the existence and nonexistence of ground state solution for the system with non-periodic couplings via Nehari manifold method. Especially, the ground state solution with both nontrivial components is obtained, and the sign of nontrivial components is considered.
Comparisons of improved genomic predictions generated by different imputation methods for genotyping by sequencing data in livestock populations
Authors: Wang Xiao , Su Guosheng , Hao Dan , Lund Mogens Sandø , Kadarmideen Haja N.
Published: February 2020

Background

Genotyping by sequencing (GBS) still has problems with missing genotypes. Imputation is important for using GBS for genomic predictions, especially for low depths, due to the large number of missing genotypes. Minor allele frequency (MAF) is widely used as a marker data editing criteria for genomic predictions. In this study, three imputation methods (Beagle, IMPUTE2 and FImpute software) based on four MAF editing criteria were investigated with regard to imputation accuracy of missing genotypes and accuracy of genomic predictions, based on simulated data of livestock population.

Results

Four MAFs (no MAF limit, MAF ≥ 0.001, MAF ≥ 0.01 and MAF ≥ 0.03) were used for editing marker data before imputation. Beagle, IMPUTE2 and FImpute software were applied to impute the original GBS. Additionally, IMPUTE2 also imputed the expected genotype dosage after genotype correction (GcIM). The reliability of genomic predictions was calculated using GBS and imputed GBS data. The results showed that imputation accuracies were the same for the three imputation methods, except for the data of sequencing read depth (depth) = 2, where FImpute had a slightly lower imputation accuracy than Beagle and IMPUTE2. GcIM was observed to be the best for all of the imputations at depth = 4, 5 and 10, but the worst for depth = 2. For genomic prediction, retaining more SNPs with no MAF limit resulted in higher reliability. As the depth increased to 10, the prediction reliabilities approached those using true genotypes in the GBS loci. Beagle and IMPUTE2 had the largest increases in prediction reliability of 5 percentage points, and FImpute gained 3 percentage points at depth = 2. The best prediction was observed at depth = 4, 5 and 10 using GcIM, but the worst prediction was also observed using GcIM at depth = 2.

Conclusions

The current study showed that imputation accuracies were relatively low for GBS with low depths and high for GBS with high depths. Imputation resulted in larger gains in the reliability of genomic predictions for GBS with lower depths. These results suggest that the application of IMPUTE2, based on a corrected GBS (GcIM) to improve genomic predictions for higher depths, and FImpute software could be a good alternative for routine imputation.
Is there an association between full IQ score and mental health problems in young adults? A study with a convenience sample
Authors: Melby Linde , Indredavik Marit S. , Løhaugen Gro , Brubakk Ann Mari , Skranes Jon , Vik Torstein
Published: January 2020

Background

Intelligence is the aggregate or global capacity of the individual to act purposefully, to think rationally and to deal effectively with the environment. Previous studies have shown that individuals with intellectual disability, IQ < 70, have increased risk of being diagnosed with one or more mental disorders. We wanted to investigate if this also applies to individuals with IQ between 70 and 85.

Methods

In this study, data was abstracted from a longitudinal follow-up study of individuals with low birth weight and a control group. In the present study, mental health of participants with borderline IQ, defined as a full IQ score 70–84, were compared with mental health of a reference group with full IQ scores ≥85. Mental health at age 19 was assessed using the Schedule for Affective Disorder and Schizophrenia for School-age Children Present and Lifetime (K-SADS P/L) whereby scores meeting the diagnostic criteria for a mental disorder were defined as having mental health problems. In addition the participants completed the ADHD-rating scale and the Autism Spectrum Quotient form (AQ). Logistic regression analyses were used to calculate odds ratio (OR) with 95% confidence intervals (CI) for high scores on the K-SADS.

Results

Thirty participants with borderline IQ and 146 controls were included. Sixteen (53%) of the participants with borderline IQ met the diagnostic criteria on the K-SADS for any diagnosis compared with 18 (12%) in the reference group (OR: 6.2; CI: 2.6–14.9). In particular the participants with borderline IQ had excess risk of ADHD and anxiety. These associations were slightly attenuated when adjusted for birth weight and parents’ socioeconomic status.

Conclusions

53% of the participants with borderline IQ had increased risk for a research assessed psychiatric diagnosis compared to about one in ten in the reference group. The group with borderline IQ also had higher total scores and higher scores on some sub-scores included in the Autism Spectrum Quotient form. Our results points towards an increased vulnerability for mental illness in individuals with borderline low IQ.

Trial registration

The main study is recorded by the Regional Committee for Health Research Ethics in Mid-Norway (as project number 4.2005.2605).
Wwox deficiency leads to neurodevelopmental and degenerative neuropathies and glycogen synthase kinase 3β-mediated epileptic seizure activity in mice
Authors: Cheng Ya-Yun , Chou Ying-Tsen , Lai Feng-Jie , Jan Ming-Shiou , Chang Tsung-Hao , Jou I-Ming , Chen Pei-Shiuan , Lo Jui-Yen , Huang Shiang-Suo , Chang Nan-Shan , Liou Yung-Tsai , Hsu Po-Chih , Cheng Hui-Ching , Lin Yee-Shin , Hsu Li-Jin
Published: January 2020

Human WWOX gene resides in the chromosomal common fragile site FRA16D and encodes a tumor suppressor WW domain-containing oxidoreductase. Loss-of-function mutations in both alleles of WWOX gene lead to autosomal recessive abnormalities in pediatric patients from consanguineous families, including microcephaly, cerebellar ataxia with epilepsy, mental retardation, retinal degeneration, developmental delay and early death. Here, we report that targeted disruption of Wwox gene in mice causes neurodevelopmental disorders, encompassing abnormal neuronal differentiation and migration in the brain. Cerebral malformations, such as microcephaly and incomplete separation of the hemispheres by a partial interhemispheric fissure, neuronal disorganization and heterotopia, and defective cerebellar midline fusion are observed in Wwox−/− mice. Degenerative alterations including severe hypomyelination in the central nervous system, optic nerve atrophy, Purkinje cell loss and granular cell apoptosis in the cerebellum, and peripheral nerve demyelination due to Schwann cell apoptosis correspond to reduced amplitudes and a latency prolongation of transcranial motor evoked potentials, motor deficits and gait ataxia in Wwox−/− mice. Wwox gene ablation leads to the occurrence of spontaneous epilepsy and increased susceptibility to pilocarpine- and pentylenetetrazol (PTZ)-induced seizures in preweaning mice. We determined that a significantly increased activation of glycogen synthase kinase 3β (GSK3β) occurs in Wwox−/− mouse cerebral cortex, hippocampus and cerebellum. Inhibition of GSK3β by lithium ion significantly abolishes the onset of PTZ-induced seizure in Wwox−/− mice. Together, our findings reveal that the neurodevelopmental and neurodegenerative deficits in Wwox knockout mice strikingly recapitulate the key features of human neuropathies, and that targeting GSK3β with lithium ion ameliorates epilepsy.
Sustainability problems of the Giza pyramids
Authors: Hemeda Sayed , Sonbol Alghreeb
Published: January 2020

The Pyramids complex in Giza consists of three main pyramids in addition to the famous Sphinx and small queen’s pyramids. Recently, the pyramids of Cheops (Khufu), Chephren (Khafre) and Mykerinos (Menkaure) on the Giza plateau have been threatened by a rising groundwater table resulting from water leakage from the suburbs irrigation canals, and mass urbanization surrounding the Giza pyramids. The pyramids at Giza suffer from a lot of Geo-environmental and structural problems. The main objectives of this study are (1) to assess the current status of the preservation of this unique and high valuable archaeological site, (2) to analyze the various actions that cause the destruction of the pyramid complex, in particular the weathering activities and strong seismic event, and (3) to determine the geochemical and engineering properties for construction materials using different types of tools and advanced analytical and diagnostic techniques. Structural stability analysis requires good assessment of present conditions of major materials used such as stones and structural mortar. The paper shows a thorough analysis of the current condition of the Great Pyramids at Giza. The work includes a discussion and analysis of the natural character and source of the pyramids building stones, geological context, damage survey, petrographic investigation, and physical and mechanical characterization of the stones and structural mortars, by means of laboratory and in situ testing. The results are displayed, described and analyzed in the paper in the context of potential threats to the monuments. The experimental study indicates the dependence of mechanical geological properties on the physical properties and the mineral composition of the studied building materials. The physical and petrographic characteristic of the stones are related. The modeling of properties indicates a reliable relationship between the various visible pores and uniaxial compression force parameters that can be applied to predict and characterize limestone elsewhere.