bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental restrictions in territorial planning schemes of Russian agglomerations
Authors: Ageeva I. V. , Volkova I. N. , Krylov P. M.
Published: April 2023

Aim. We consider the environmental restrictions in the development of agglomerations on the territory of Russia, which are taken into account or should be taken into account in the process of developing the schemes for their territorial planning.

Methodology. The work makes use of official statistical data, information from state authorities and local governments, materials from Development Concepts and Territorial Planning Schemes (TPSs) of a number of Russian agglomerations, and the results of the 2021 All-Russian Population Census. As the keys, we study TPSs of Samara-Togliatti, Kavminvodsk and Novosibirsk agglomerations. Various environmental restrictions taken into account in the development of TPSs of agglomerations, selected as the keys for this study, are compared and analyzed.

Results. The main results of the work include the compilation of a list of environmental restrictions on territorial development used in a number of TPSs for agglomerations developed in our country in recent years with references to their legal regime, as well as a comparative analysis of the use of certain environmental restrictions in the development of TPSs of agglomerations located in different types of Russian regions. The analysis shows that the list of environmental restrictions taken into account in the development of the TPS of a particular agglomeration depends not only on the natural, economic-geographical or urban specifics of the planning object. In a number of cases, unreasonable omissions of some restrictions taken into account in other TPSs are noted. The absence of a clearly regulated list of environmental restrictions approved by experts in this field of activity, as well as a unified form for submitting such materials to the TPS of each agglomeration, in our opinion, requires serious improvement.

Research implications. The work is of practical importance, since the analysis of the developed TPSs of Russian agglomerations in regions of various types and, based on its results, a fairly complete (representative) list of the environmental restrictions of prospective territorial development used in them allows using this material for further work in this type of territorial planning. The data on the legal regime of each type of environmental restrictions given in the same list will make it possible to use them in the future in the development of regional strategic documents and territorial planning documents.
New geography of the world pharmaceutical industry: trends of the development
Authors: Rodionova I. A. , Aidrous I. A.
Published: April 2023

Aim. The purpose is to reveal the spatial regrouping of forces in the architecture of the global pharmaceutical industry at regional and global levels.

Methodology. The analysis of available sources (UNIDO. Industrial Development Report 2020; “Torreya’s Pharma 1000 Report. 2021”; “COVID-19: The Great Reset” 2020 and others) was carried out. Statistical data (“Science and Engineering Indicators” 2022; “Global pharmaceutical industry – statistics & facts” 2022; “Fortune Global 500”) were used. А comparative analysis of indicators in the dynamics of the beginning of the 21st century was applied.

Results. Spatial changes in the global pharmaceutical industry since the beginning of the 21st century are assessed. The regions-leaders and countries-leaders of the industry are designated. The role and positions of the largest pharmaceutical corporations in the Fortune Global 500 rating are characterized. The high level of monopolization of the industry’s production capacities is shown. The main directions of the development of the world pharmaceutical industry are outlined. The factors determining them are indicated.

Research implications. The Asian region is currently the leader in the global pharmaceutical industry. However, the United States holds the leading position in the pharmaceutical industry. But China has already taken the 2nd place in the world, ahead of many highly developed countries. At the same time, China’s share in the output of the pharmaceutical industry in the Asian region exceeds 65%. Theoretical and practical significance of the study on identifying the spatial regrouping of forces in the global pharmaceutical industry is due to the fact that the recommendations on using the experience of industry development in the leading countries (primarily in China) can be used by Russian pharmaceutical companies and regulatory authorities in the healthcare sector. The results of the paper can be used in the educational process in universities.
Molecular Characterization of Odorant Receptor Gene GmolOR7 in Grapholita molesta (Lepidoptera: Tortricidae)
Authors: Chen Li-Hui , Li Mei-Mei , Li Yan-Ying , Cheng Wei-Ning , Wei Hong-Yi , Xu Xiang-Li , Wu Jun-Xiang
Published: April 2023

Grapholita molesta Busck (Lepidoptera: Tortricidae) is a major fruit pest of a wide range of Rosaceae trees. Olfaction plays a crucial role in insect behavioral activities, and insects primarily rely on odorant receptors (ORs) to detect odorant molecules. Several olfactory-related genes in G. molesta have been studied; however, the ORs of G. molesta are unclear. Here, GmolOR7, a general OR gene, was cloned and characterized. Sequence alignment and phylogenetic tree analysis showed that GmolOR7 is closely related to Cydia pomonella OR54, with the amino acid sequence identity of 80.21%. Furthermore, the different developmental stages and tissues of GmolOR7 in G. molesta were analyzed by real-time quantitative polymerase chain reaction. The results demonstrated that GmolOR7 was expressed at higher levels in adults, and particularly in the antennae of females. GmolOR7 expression was the highest in 3-d-old adults. These results will provide an indication for further functional study of the GmolOR7 in G. molesta and its role in the detection of host-plant volatiles by insects in general.
State-owned enterprises and economic growth in interaction models: Role of institutional quality
Authors: Hoang Dinh Cong , Tuan Dinh Cong
Published: April 2023

China’s institutional quality and organizational structure have been altered as a result of the 4.0 industrial revolution. Because of this, the role of state-owned enterprises (SOEs) has become even more important since the arrival of the fourth industrial revolution. This study explores whether or not there is a complementarity between SOEs and the quality of institutions in terms of their influence on economic growth for selected regions in China. The data on SOEs is collected for 29 regions (provinces, autonomous regions, or municipalities) of China over the period of 2001 to 2020. The study uses the Cross-Sectional Autoregressive Distributive Lag model (CS-ARDL) approach to estimate the model. The findings indicate that the effect of SOEs is conditioned on the quality of institutions. Our analysis reveals that the quality of a nation's institutions is an important factor to consider when evaluating the effectiveness of SOEs in spurring economic growth.
The Effect of Digital Leadership, organizational culture, digital competence and organization's commitment on Organizational Performance: Information Technology System in Indonesian Navy
Authors: T Puliwarna , Djati S. Pantja , Tanti P. Elisabeth
Published: April 2023

The phenomenon of organizational performance within the TNI AL Headquarters has not been optimally assisted by the existence of existing information technology. The work process becomes less responsive because a lot is done manually. The purpose of this study is to explain the influence of digital leadership, digital competence and digital culture of crew members to improve the performance of the Indonesian Navy's organizations. The research sample was taken by purposive and cluster random sampling by selecting respondents who became operators or were involved in the use of information systems in their work units. The total sample of 445 respondents was obtained from 11 Navy's organizations of the Indonesian Navy Headquarters, which in total had a crew of 1,400 people, but the population involved in IT operations was approximately 800 people. The results of this study indicate that digital leadership has a direct positive and insignificant effect on organizational performance, The direct effect is shown in the positive and insignificant direct influence of digital leadership on organizational performance; while the direct effect is positive and significant digital competence, and organizational commitment to organizational performance; negatively and significantly direct effect of digital culture on organizational performance within the Indonesian Navy Headquarters. Digital competence directly has a positive and significant effect on organizational performance as well as on organizational commitment. Digital culture has a direct and significant negative effect on organizational performance and organizational commitment. The novelty of this research, Organizational commitment has a direct positive and significant effect on organizational performance
Podjetniška demografija in značilnosti startup in scaleup podjetij: Slovenski podjetniški observatorij 2022
Authors: Bradač Hojnik Barbara
Published: April 2023

V prvem delu monografije smo proučevali temeljno poslovno demografijo slovenskih podjetij in jo primerjali z evropsko. Namen tega dela je, da dobimo temeljno sliko o slovenskih podjetjih in razumemo, kako je slovensko podjetništvo umeščeno v evropsko. Analizirali smo ključne značilnosti slovenskih gospodarskih družb in samostojnih podjetnikov, ki so se leta 2021 ukvarjali s podjetniškimi aktivnostmi in so bili razvrščeni v vse dejavnosti SKD. V drugem delu raziskave smo proučevali značilnosti startup in scaleup podjetij. Osredotočili smo se predvsem na proučevanje nihovih značilnosti, potreb in izzivov, saj podjetja na začetku razvoja in pri prehodu v rast potrebujejo največ podpore in spodbud. Proučili smo njihov strateški pomen, naredili mednarodno primerjavo startup in scaleup podjetij, analizirali podporno okolje zanje ter na osnovi primarne raziskave med njimi podali ključne značilnosti ter priporočila za njihovo podporo in razvoj v Sloveniji.
On microbial landscape of palatine tonsils in patients with chronic tonsillitis associated with chronic opisthorchiasis invasion
Authors:   Tyumen State Medical University, Tyumen, 625023, Russia, Izvin A. I. , Rudzevich A. V. ,   Tyumen State Medical University, Tyumen, 625023, Russia,   Regional Clinical Hospital No. 2, Tyumen, 625039, Russia
Published: April 2023

The palatine tonsils, both in normal conditions and in chronic tonsillitis (CT), can be carriers of a diverse, including pathogenic, flora, which becomes virulent under the influence of various adverse factors of the external and internal environment. Bacteriological studies carried out by CT patients show a wide variety of vegetative flora in the palatine tonsils. However, according to many researchers, the leading microbial agent is group A B-hemolytic streptococcus. Objective. To study the microbial landscape of palatine tonsils in patients with chronic tonsillitis associated with chronic opisthorchiasis and without the latter. Patients and methods. The authors studied the microbial landscape in 106 patients with chronic tonsillitis aged 20 to 55 years who were randomized into 2 groups (the main 56 people with CT associated with chronic opisthorchiasis invasion (COI)) and the control group (50 patients without the presence of the latter). Conclusions. It was established that the microbial landscape of the palatine tonsils in patients with chronic tonsillitis in association with COI is characterized by significant polymorphism with the predominant role of staphylococci and streptococci, with the lacunar microflora being more virulent, while the microflora of the palatine tonsils in patients without COI did not significantly differ from the same as in the control group, but it was less virulent.
Tactics of treatment of exacerbations of chronic rhinosinusitis in patients with polypous rhinosinusitis in combination with allergic rhinitis
Authors:   Vladimirsky Moscow Regional Research Clinical Institute, Moscow, 129110, Russia, Shachnev K. N. , Egorov V. I. ,   Vladimirsky Moscow Regional Research Clinical Institute, Moscow, 129110, Russia, Savushkina E. Yu. ,   Vladimirsky Moscow Regional Research Clinical Institute, Moscow, 129110, Russia
Published: April 2023

The relationship between polypous rhinosinusitis and allergic rhinitis, as well as the formation of tactics for the treatment of exacerbations, is a prominent problem of modern otorhinolaryngology. Changes of the microbiota of the nasal cavity and paranasal sinuses, the addition of a bacterial infection are the risk factors in the development of exacerbations of chronic purulent rhinosinusitis. Of particular difficulty is the formation of bacterial biofilms, as well as the presence of concomitant pathology that worsens the course of an exacerbation of the disease and additional methods of treatment. Our goal was to develop and test in clinical practice a personalized treatment regimen for exacerbations of chronic purulent rhinosinusitis in patients with polyposis rhinosinusitis in combination with allergic rhinitis, as well as to determine its effectiveness. A prospective study was conducted on a cohort of 19 patients with polypous rhinosinusitis in combination with allergic rhinitis. During the study, the bacteriological composition of the microflora of the nasal cavity and paranasal sinuses was assessed, a thorough examination of patients was carried out, treatment was prescribed in accordance with the severity and the developed scheme. Control studies were carried out on the 7th day of the study, if necessary, the treatment tactics were modified. By the 14th day of the study, patients showed normalization of the endoscopic picture, the disappearance of the symptoms of the disease, as well as the restoration of the microflora of the nasal cavity with a decrease in the level of pathogenic and opportunistic microorganisms. Thus, the need for complex diagnostics and personalized treatment in exacerbation of chronic purulent rhinosinusitis in patients with polypous rhinosinusitis in combination with allergic rhinitis was shown, and the effectiveness of the developed treatment regimen was confirmed by both subjective and objective methods.
Case report of rare case of foreign body in laryngopharynx
Authors:   Kursk State Medical University, Kursk, 305041, Russia, Chestnikova S. E. , Nikitin N. A. ,   Kursk Regional Multidisciplinary Clinical Hospital, Kursk, 305007, Russia, Piskunov V. S. ,   Kursk State Medical University, Kursk, 305041, Russia, Piskunov I. S. ,   Kursk State Medical University, Kursk, 305041, Russia, Levchenko A. S. ,   Kursk State Medical University, Kursk, 305041, Russia, Mezentseva O. Yu. ,   Kursk State Medical University, Kursk, 305041, Russia
Published: April 2023

Foreign bodies of the larynx, pharynx, and laryngopharynx are quite common cases in otorhinolaryngological practice. At the same time, they make up a significant proportion of emergency surgical interventions among other ENT pathologies. Foreign bodies can damage the walls of the organ, causing dangerous complications. Diagnosis is usually based on data from laryngoscopy or endoscopy. However, in rare cases, additional research methods, such as computed tomography, are required. With deep localization of foreign bodies of the laryngopharynx, their removal is difficult, as a result of which they resort to surgical intervention. This article is a description of a foreign body (chicken bone) of unusual localization in a 57-year-old female patient. The thin sharp bone migrated entirely into the soft tissues of the left pyriform sinus, which was diagnosed by computed tomography. Since it was not possible to remove the foreign body under the control of laryngoscopy, it was decided to perform a left-sided colotomy. After a successful operation, the patient’s condition was satisfactory, the wound healed by primary intention, the sutures were removed on the seventh day after the operation. The article presents the data upon admission of the patient as well as the course of treatment illustrated by the picture of changes presented on tomography scans.
Pneumatic controllers based on the jet force action effect
Authors: Makarov V.A. , Kazaryan A.Z. , Korolev F.A.
Published: April 2023

The paper considers schemes of pneumatic regulators, which operation principle is based on the jet force action effect on the flat plate. The main nodes of such regulators include the plate support and the compensatory circuit of the regenerative feedback forming the basis for building pneumatic control circuits that implement the basic laws of regulation. The proposed devices are able to improve static characteristics due to their linearity. Schemes are provided of aerostatic bearing, regenerative feedback, jet-pneumatic proportional and proportional-integral controllers, precession and differentiation block, proportional integral differential controller and semi-proportional controller.
Introduction of multicenter design in the non-clinical phase to overcome translational barriers in clinical trials
Authors: Murazov Ia. G. , Kovaleva M. A. , Makarova M. N. , Makarov V. G.
Published: April 2023

Background. The translating of individual preclinical studies (PCTs) results into clinical practice is accompanied by a significant number of failures and is associated with significant time and financial costs. The unsatisfactory design of individual non-clinical studies and the reports submitted after having done in vivo experiments at one laboratories do not allow overcoming translational barriers and increasing the results reproducibility. To conduct multicenter non-clinical trials is relevant to to confirm the effectiveness of medicines and increase the chances of successful translation of non-clinical results into clinical practice.

Objective. To analyze the scientific literature devoted to multicenter non-clinical studies in the research of the pharmacological activity of new drugs.

Design and methods. The research was done using PubMed and Google Scholar databases. The review included available publications as of October 16, 2022.

Results. It was discovered that single-center studies did not allow predicting the magnitude of the effect accurately, and large sample sizes made the estimation of the magnitude of the effect even less precise.

Conclusions. The main purpose of multicenter non-clinical studies rather should be assessing the direction of the effect, including its “stability”, as well as to identify sources of heterogeneity between studies conducted in different laboratories (centers) than performing a quantitative assessment of the effect on several laboratories, as it tend to be in foreign non-clinical practice.
The Classical Conception of Truth in the Light of Hegel's "Logic“
Authors: Kozłowski Marek
Published: April 2023

Ponieważ każda księga heglowskiej "Logiki" przedstawia tylko jej właściwy sposób przejawiania się bytu, więc zakłada ona także - określoną przez ten sposób przejawiania się bytu - charakterystyczną wykładnię prawdy. Niedowolna kolejność poszczególnych ksiąg określa wtedy także porządek, w jaki układają się założone w nich wykładnie prawdy. Skorelowanie klasycznej i innych wykładni prawdy z odpowiednimi księgami "Logiki" pozwala więc wykorzystać porządek "Logiki” do odtworzenia struktury całości prawdy, tzn. także do objaśnienia miejsca, jakie klasyczna wykładnia zajmuje zarówno w obrębie całości prawdy, jak i wśród wykładni pozostałych.
ПРИНЦИПЫ АНАЛИЗА И ТИПОЛОГИЯ В МЕТОДОЛОГИИ ЭКОНОМИЧЕСКИХ ШОКОВ
Authors: Жиронкин Сергей Александрович , Таран Екатерина Александровна , Алешина Ольга Геннадьевна
Published: April 2023

В настоящее время распространенным явлением в экономике является глобальное усиление разнообразных флуктуаций, которые носят как циклический, так и нециклический – шоковый – характер. Предметом современных дискуссий часто выступает обсуждение краткосрочности шокового воздействия и эффективности рыночного механизма в условиях шоков. Преодоление последствий воздействия различных шоков на экономику, как и формирование ее шокоустойчивости, ассоциируется большинством авторов с повышением гибкости рынков, наращиванием государственных расходов и масштабными денежными эмиссиями – традиционными антициклическими инструментами. Вместе с тем сегодня наиболее разрушительной силой отличаются экзогенные шоки (технологические прорывы и ограничения, пандемия, санкции и эмбарго), которые, будучи посторонними по отношению к экономической системе, недостаточно чувствительны к экономическим инструментам противодействия. Цель: дополнение существующей методологии экономических шоков новыми принципами и типологией, отражающими их связь со структурными изменениями – залогом шокоустойчивости экономики. Методы: анализ работ российских и зарубежных авторов в области исследования, обобщение научных подходов и критический анализ теоретических положений. Результаты представляют собой типологию экономических шоков, структурированную в разрезе ряда принципов их исследования и критериев спецификации, которые образуют методологию нециклических явлений в экономике. Конструктивная критика существующих подходов позволила разработать теоретическое положение о связи экономических шоков со структурой экономики, позитивные изменения в которой должны лечь в основу антишокового регулирования. Выводы: существующие подходы к анализу экономических шоков адекватно описывают их влияние на экономические циклы и основаны на главном достижении современной рыночной экономики – гибкости рынков и их регулировании. В то же время наблюдаемые сегодня наиболее сильные экзогенные шоки, воздействие которых на трансформационную российскую экономику может стать разрушительным, актуализируют поиск структурных принципов противодействия им, которое не может быть организовано без соответствующей теории и методологии, важной частью которой является структурно-обусловленная типология шоков.
Homenaje a Félix Denegri Luna
Authors: Aparicio Quispe Severo
Published: April 2023

Ente los historiadores peruanos de los últimos tiempos se destaca con perfil propio Félix Denegrí Luna, limeño de nacimiento, abogado de profesión y consagrado con amorosa dedicación al estudio de la historia patria. Por mi prolongada permanencia en el extranjero, conocí tarde a este historiador de excelsas cualidades humanas y cívicas. Desde que nos conocimos se entabló entre él y el suscrito noble y entrañable amistad. Quienes le conocieron de más cerca y por más tiempo que yo, podrán ocuparse de Félix Denegrí con más autoridad de su ejemplar trayectoria humana y de su generosa contribución a la cultura nacional. Con todo, en esta ocasión quiero expresar el testimonio de mi admiración a las altas cualidades humanas de don Félix, hombre afectuoso, acogedor, caballero a carta cabal y amigo de sus amigos. A veces nos toca valorar a las personas cuando ya no están entre nosotros.
Evocación de don Félix Denegri Luna
Authors: Sanz Luis Santiago
Published: April 2023

El historiador peruano Félix Denegri Luna falleció en el Ecuador el 6 de diciembre de 1998, a los 79 años. Era miembro correspondiente de nuestra corporación. Participó en el VI Congreso Internacional de Historia de América, celebrado en 1980 en Buenos Aires. En esa oportunidad leyó un trabajo vinculado con un tema que por entonces estaba en el centro de sus estudios. Lo tituló Cusco. Sus fundaciones y su rol en la formación del nacionalismo hispanoamericano. Poco antes había prologado la obra de Diego de Esquivel y Navia Noticias Cronológicas de la gran ciudad del Cusco, escrita en el siglo XVIII.
Félix Denegri Luna
Authors: Salvador Lara Jorge
Published: April 2023

El III Congreso Nacional de Historia, que con tanto éxito se realizó en Quito con asistencia de numerosos investigadores ecuatorianos y extranjeros, terminó con una nota dolorosa: el infarto cardiaco que afectó a uno de sus más connotados participantes, el historiador peruano doctor Félix Denegri. Operado de urgencia, el buen éxito de la intervención quirúrgica hacía presumir su pronta convalecencia, no obstante hallarse próximo a los 80 años de edad; pero aunque él deambulaba como un joven en el Cuzco o La Paz, no logró finalmente resistir la presión de la altiplanicie quiteña y falleció en esta ciudad, que había visitado tantas veces y a la que admiraba. En medio del amor y dolor de su familia, que vino ante la emergencia, y de sus amigos, sus restos mortales fueron cremados y volvieron a Lima para recibir cristiana sepultura.
Discurso en el acto de homenaje ofrecido por la Academia Nacional de la Historia en el Museo Nacional de Antropología, Arqueología e Historia del Perú
Authors: Nieto Vélez Armando
Published: April 2023

El 6 de diciembre del año pasado falleció en Quito Félix Denegri Luna, miembro ilustre de nuestra Academia. En su memoria nos reunimos esta noche para evocar al colega y al amigo, y para poner de relieve la importancia de su obra historiográfica, que me atrevo a calificar de imprescindible dentro del acervo de aportes sustantivos a la historia de la República.
Vida y obra de Juan Pablo Viscardo y Guzmán : precursor de la Independencia de Hispanoamérica
Authors: de Belaunde Ruiz de Somocurcio Javier
Published: April 2023

Juan Pablo Viscardo y Guzmán, peruano inmortal, es una de las más notables personalidades que han brillado en el escenario del acontecer humano. Nació el 26 de junio de 1748 en la hermosa villa de Pampacolca. Por ello, esta población estará siempre presente en la historia: la gloria de su hijo impar la ha inmortalizado.
Moquegua ante los retos de la modernidad : sociedad urbana y producción en el siglo XIX
Authors: Cañedo-Argüelles Fabrega Teresa
Published: April 2023

Los albores del siglo XIX coincidieron con la difusión de un nuevo bagaje de ideas que circulaban desde la lejana Europa hasta los parajes más recónditos de América, por cauces que se abrían y se multiplicaban formando una tupida red de significados contradictorios. Por una parte los conceptos de «monarquía» y «patria» inundaban las conciencias de los súbditos americanos, en un desesperado intento de restañar las fisuras que agrietaban la máquina imperial, mientras que por otra la «masonería», bajo su lema de fraternidad universal, contribuía a activarlas con la propagación de ideas antimonárquicas e independentistas y con las que todo el continente sintonizó en una magna experiencia revolucionaria.
Screening of Different Bioagents and Fungicides against Dry Root Rot of Chickpea Incited by Macrophomina phaseolina (Tassi) Goid
Authors: Malagi Nagaraj C. , Kumhar Data Ram , Yadav A.L. , Kumar Vikash , Kumar Rakesh , Choudhary Anand , Mimrot Mahesh Kumar
Published: April 2023

Background: Dry root rot of chickpea caused by Macrophomina phaseolina (Tassi) Goid is a serious biotic constraint for chickpea production in Rajasthan. For the management of soil borne disease like dry root rot of chickpea, by using fungicides alone is not feasible due to environmental and health hazards. Hence integrated management of the disease by using bio-agents and fungicides is the best alternative. Therefore, in the present investigation, our main emphasis was to identify best fungicide and bio-agent for management of dry root rot in chickpea. Methods: Eight fungicides and four bio-agents were evaluated against dry root rot pathogen (M. phaseolina) in lab as well as in field condition. The experiment was conducted at instructional farm, COA, SKRAU, Bikaner during Rabi-2019 on most popular cv. GNG-1581 in RBD design with the application of seed treatment and soil application of different bio-agents and fungicides at different concentrations against dry root rot disease and compared with an untreated control. Result: Among all the fungicides used in the present investigation, tebuconazole 50% + trifloxystrobin 25% was found most effective in inhibiting the mycelial growth of the pathogen. Among all the bio-agents, T. harzianum was found most effective in inhibiting the mycelial growth of the pathogen. Under field condition, tebuconazole 50%+ trifloxystrobin 25% WG as seed treatment @ 1.5 g/kg along with T. harzianum @ 10 kg/ha as soil application gave maximum (83.76 %) disease control with highest pod yield (19.5 q/ha) and net return (Rs 39,826/ha). These treatments can provide an effective and economical management of dry root rot disease for chickpea cultivators.
Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China
Authors: Zhang XiaoLing , Jia Siyuan , Cui LuYue , Yang Hong , Yi Li
Published: April 2023

Background: Escherichia coli (E. coli) is a pathogen that sickens both humans and animals. There are relatively few reports on the correlation between drug resistance and biofilm formation ability of E.coli isolated from goose and fish in water environment of China. This study investigated the genetic typing, antibiotic sensitivity and biofilm formation ability of E. coli strains isolated from goose and fish. Methods: The phylogenetic clustering, drug resistance and biofilm formation ability of E. coli were tested by triple PCR, crystal violet microplate and Microdilution method. Result: The results showed the prevalence of group B2 and D in fish-derived E.coli accounted for 59% and 12%, goose-derived E. coli accounted for 48% and 9%, respectively. Some strains could form biofilm and biofilm formation ability was associated with the drug resistance. The MIC values of strains growing in biofilm were 2-16 times higher than those of corresponding planktonic bacteria. The antibiotic resistance among biofilm-forming isolates was significantly higher than that strains unable to form biofilm (p less than 0.05).
Biodistribution of therapeutic extracellular vesicles
Authors: Gupta Dhanu , Wiklander Oscar P.B , Wood Matthew J.A , El-Andaloussi Samir
Published: April 2023

The field of extracellular vesicles (EVs) has seen a tremendous paradigm shift in the past two decades, from being regarded as cellular waste bags to being considered essential mediators in intercellular communication. Their unique ability to transfer macromolecules across cells and biological barriers has made them a rising star in drug delivery. Mounting evidence suggests that EVs can be explored as efficient drug delivery vehicles for a range of therapeutic macromolecules. In contrast to many synthetic delivery systems, these vesicles appear exceptionally well tolerated in vivo. This tremendous development in the therapeutic application of EVs has been made through technological advancement in labelling and understanding the in vivo biodistribution of EVs. Here in this review, we have summarised the recent findings in EV in vivo pharmacokinetics and discussed various biological barriers that need to be surpassed to achieve tissue-specific delivery.
Senolytics rejuvenate the reparative activity of human cardiomyocytes and endothelial cells
Authors: Sunderland Piotr , Alshammari Lulwah , Ambrose Emily , Torella Daniele , Ellison-Hughes Georgina M.
Published: April 2023

Introduction: Senescent cells have emerged as bona fide drivers of ageing and age-related cardiovascular disease, with senescent cells accumulating in the aged heart and following damage/injury. We have shown that the removal of senescent cells using senolytics can rejuvenate the regenerative capacity of the aged heart. Aim: To investigate the effects of cell senescence and the action of the senolytics, Dasatinib (D) and Quercetin (Q) on human iPSC-derived cardiomyocyte survival and cell cycle, and endothelial cell survival, cell cycle, migration and tube formation in vitro. Methods and Results: We developed a transwell insert co-culture stress-induced premature senescence human cell model system to test the effects of senolytics D+Q in vitro. Co-culture of iPSC-derived cardiomyocytes (iPSC-CMs) with senescent cardiac stromal progenitor cells (senCPCs) led to decreased number and DNA-synthesising activity of iPSC-CMs. Treatment with senolytics D+Q led to the elimination of senCPCs in the co-culture and the rescue of iPSC-CM number and DNA synthesis. Treatment of HUVECs with senCPC conditioned media decreased HUVEC number, cell cycle activity, migration, and tube formation. Treatment of HUVECs with D+Q conditioned media rescued HUVEC number, migration and tube formation. Next, we investigated the effects of co-culture of senescent HUVECs (senHUVECs) with HUVECs and showed decreased HUVEC number and DNA synthesis. Treatment with senolytics D+Q led to the elimination of senHUVECs in the co-culture and ameliorated HUVEC number, but not DNA synthesis. Treatment of HUVECs with conditioned media from senHUVECs led to decreased HUVEC migration and tube formation. Treatment of HUVECs with D+Q conditioned media improved HUVEC tube formation but not migration. Luminex analysis of the conditioned media from iPSC-CM and HUVEC co-cultures revealed upregulation of senescence-associated secretory phenotype (SASP) factors, but the level of SASP factors was reduced with the application of D+Q. Conclusion: Senescent cell removal by senolytics D+Q shows therapeutic potential in rejuvenating the reparative activity of human cardiomyocytes and endothelial cells. These results open the path to further studies on using senolytic therapy in age-related cardiac deterioration and rejuvenation. Potential impact of the findings: Senescent cells and their SASP present a promising therapeutic target to rejuvenate the heart’s reparative potential. Clinical trials using senolytics D+Q are already underway and thus far have shown promising results. Further pre-clinical studies are warranted for evidence-based clinical trials using senolytics in age-related cardiovascular diseases.
The Experience of Type II Diabetes Mellitus Patients with Early Symptoms of Hypoglycemia: A Phenomenological Study
Authors: Nurjannah Misbah , Asthiningsih Ni Wayan Wiwin
Published: April 2023

Hypoglycemia is one of the acute complications that often occur in diabetes mellitus (DM) patients and could become an emergency condition that requires immediate treatment. The initial symptoms of hypoglycemia are cold sweats, tremors, dizziness, and blurred vision. Generally, DM patients’ lack of diet management exacerbates hypoglycemia. This study aimed to explore experience of type 2 diabetes patient’s hypoglycemia symptoms and affected factors. This qualitative research applied a phenomenological approach to seven participants. Semi-structured interviews were done to collect data. The Braun & Clarke method was then used to analyze the data. This study revealed three themes including the initial hypoglycemia symptoms, diet modification, and medication adherence. The study’s results are expected to provide information on the importance of education and control related to initial hypoglycemia symptoms, nutrition, and medications awareness to the type 2 diabetes patients and family.
A The Determinant Factors of Patient Satisfaction among Outpatient and Inpatient Services in A Type B Hospital in Indonesia
Authors: Suhita Byba Melda , Fajriah Asruria Sani , Acob Joel Rey Ugsang
Published: April 2023

Healthcare providers have made various efforts to increase patient and community satisfaction with health services. However, large sample size studies have not been conducted to investigate the determinants of community stratification in Indonesia. This research aimed to analyze determinant factors affecting patient satisfaction in a type B hospital. A cross-sectional with an online survey was used and analyzed with path analysis. A total of 884 participants were recruited with the criteria that all respondents were patients aged 17 years and over who visited the inpatient and outpatient hospital. Overall, the value service satisfaction was very good (scored 87.44 score). Moreover, online service directly increased by age (b = 0.68; 95% CI = 0.08 to 1.27; p = 0.026), gender (b = 0.82; 95% CI = 0.15 up to 1.49; p = 0.016), and education level (b = 1.76; 95% CI = 0.86 to 2.66; p <0.001). Moreover, service costs are directly affected by employment (b = 0.67; 95% CI = 0.92 to 1.26; p = 0.023); and product-specific services were directly affected by age (b = 0.74; 95% CI = 0.20 to 1.29; p = 0.008). These findings suggest that education, gender, and age directly affect online services as an indicator of satisfaction.
Prevalence of Streptococcus Pneumonia and Haemophillus Influenza in primary school children that diagnosed acute otitis media
Authors: Wahyono Daniel Joko , Darmawan Anton Budhi , Mars Wijayanti Siwi Pramatama , Wibowo Yudhi , Mumpuni Aris , Krisnansari Diah , Nawangtantri Gita , Anjarwati Dwi Utami , Kusdaryanto Wahyu Dwi , Krisniawati Nia , Pramono Hendro , Pratiwi Meyta , Riza Chamadi Muhammad Riza , Octaviana Devi , Rejeki Dwi Sarwani Sri , Khoeri Mifathuddin Majid , Salsabila Korrie , Dodi Safari Dodi
Published: April 2023

Acute otitis media (AOM) remains a disease that cause major public health problem worldwide. Less information about its prevalence in Indonesia, especially in Java.  The most common bacterial causes of AOM are Streptococcus pneumoniae, non-typeable Haemophilus influenzae, and Moraxella catarrhalis. There is increasing evidence that the predominant causative pathogen in AOM is changing from Streptococcus pneumoniae to non-typeable Haemophilus influenza since the introduction of pneumococcal conjugate vaccines. This study aims to determine the prevalence of Streptococcus pneumonia and Haemophillus influenzae in AOM in primary school children in Banyumas Regency. The design of this study was cross sectional and we conducted multistage random sampling to recruit the subject. Approximately 3,574 school children in Urban Banyumas Regency were screened based on the diagnose of AOM by Otolaryngologist. The resultof this study showed that the prevalence of AOM was 4,64 % (166 children were diagnosed with AOM).  In AOM samples, the prevalence of  Streptococcus pneumonia  was found in 78,4 % by optochin test, while that of Haemophillus influenzae was found in 70,4% based on the need of  X and V factor
The effect of long germination on the levels and types of non-enzymatic antioxidants in red kidney bean sprouts
Authors: Winarsi Hery , Bestari Yudhanti Putri , Masluki Nisa Ilmi Lulu , Septiana Aisyah Tri
Published: April 2023

This study aims to explore the content of several types of non-enzymatic antioxidants in red kidney beans sprout based on duration germination. The complete randomized design study was carried out by red kidney beans sprout with germination duration variables of 0, 10, 20, and 30 hours. Red kidney bean sprouts are blended with water 8 times the amount, then filtered, so that red kidney bean sprouts extract is obtained. The phenolic content determinate using the Folin-Ciocalteu method; vitamin C testing using Iodometry; and fiber content by the proximate method. Data were analyzed using ANOVA, followed by the DMRT test if there was any significance with an error rate of 5%. The selection of products with the highest antioxidant content uses the effectiveness index test. The duration of germination increased the content of phenolic antioxidant (P <0.05), vitamin C (P <0.05), and fiber (P <0.05). Red kidney bean sprouts resulting from germination for 30 hours have the highest content antioxidants of phenolic, vitamin C, and fiber, respectively 583.85 mg GAE/L, 23.14 mg/100 mL, and 6.75%. Red kidney bean sprouts can be used as an ingredient of antioxidant-rich products that are useful for the prevention of degenerative diseases
Demographic Profile on Professional Behavior among Nursing Students in Indonesia
Authors: Susmarini Dian , Sumarwati Made , Mulyono Wastu Adi , Purnamasari Meivita Dewi , Hayati Yati Sri
Published: April 2023

Professionalism in nursing is a self-identity for every nurse. The learning of its concept begins in the academic stage through subjects and the hidden curriculum. Learning strategies have been investigated to develop professionalism values ​​among students. Aspects that affect professional behaviour need to be conveyed. This study aims to identify the correlation between the demographic profile and nurse students' professional behaviour in Indonesia. This study used a correlational design with a cross-sectional approach. A sample of 291 student nurses across Indonesia was involved and selected by quota sampling. The questionnaire used the NSPBS adapted for Indonesian students and was given through the online survey platform. The non-parametric test using the Mann-Whitney U and Kruskal Wallis tests was chosen because the data were not normally distributed. The total mean value was 92.37. The variables' test showed there was no correlation between province and sex with professional behavior with p = 0.931 and p = 0.945, respectively. Meanwhile, there were significant correlations between the age and course toward professional behavior (p = 0.007 and p = 0.012).  In this study, it was found that the older age and the extension course program showed a higher score on professional behavior. The difference is in healthcare practice and activity practice which may be shaped by work experience. Conclusion: Students' demographic profiles need more investigation as it is an antecedent of the professionalism concept
Knowledge Sharing in Peer Group: An Alternative of Internet Literacy Education Media for Children
Authors: Setiansah Mite , Novianti Wiwik , Rahmawati Anggun , Agustina Lydia
Published: April 2023

The data of the Indonesian Internet Service Provider Association in 2018 mentioned that children and teenagers aged 15-19 years old are the majority internet users in Indonesia. The first time the children access to the internet is also getting earlier from year to year. In that context, parents’ role to guide and protect their children from the online risk is getting stronger. The problem is that the parents’ internet literacy level is often lower than the children. Such a condition then push the children to search other source of information when having difficulties in accessing to the internet. The previous research mentioned that elder brothers or sisters and friends often become the reference for children related to the digital media usage. Departed from this condition, the research is conducted to explore the opportunity of utilizing peer group in literacy internet education for children. This research has been conducted in eight months involving 40 students of SMPN 2 and 40 students of SMP Al Irsyad Purwokerto as respondents. The data are collected via the survey, FGD, and indepth interview. Data analysis is conducted using descriptive and interactive analysis. The result of research shows that 61.4% of respondents admit that they prefer to choose their friends to ask and to do the knowledge sharing when having difficulties in accessing to the internet. Some reasons appearing are because the parents’ technical skill are lower than the children’s, uncommunicative talk with parents, high level of parents’ busy activities, and children’s need to socialize with their friends as amusement especially in the middle of pandemic
Implementation of standards, targets, and policy implementers at E-Warong KUBE Kenanga Sari in Banyumas Regency
Authors: Amanda Ariesta , Azriah Thahrina , Sari Lupita Ariantika
Published: April 2023

The background of this study is the high number of poor families as the family beneficiaries (KPM) of PKH in Banyumas Regency of 92,590 people. An innovation in the form of E-Warong KUBE (Electronic Shop for Mutual Business Group), such as E-Warong KUBE “Kenanga Sari” often encounters problems in the implementation process. Therefore, a study needs to be conducted on the implementation of E-Warong KUBE “Kenanga Sari”. The objective of this study was to describe the implementation of E-Warong KUBE “Kenanga Sari”, especially in terms of policy standards and objectives and the characteristics of the implementers. This study used qualitative research method. The informants were selected using purposive sampling technique. Data collection methods used interview, observation, and documentation. The results indicated that E-Warong KUBE PKH “Kenanga Sari” has been running in accordance with its objectives. All selected family beneficiaries (KPM) have been on target. However, E-Warong had no clear standards such as implementation guideline and technical guideline. The characteristics of implementers of E-Warong can be considered good. The implementation of E-Warong has been running smoothly during the Covid-19 Pandemic
Fictitious-Positive Decision Dispute Resolution at PTUN For the Achieving Unity of Proceedings and Legal Certainty
Authors: Kupita Weda , Ardhanariswari Riris
Published: April 2023

The Administrative Court (PTUN) has the authority to adjudicate ordinary state administrative disputes, namely disputes resulting from the issuance of written decisions by government officials, these disputes are classified as "ordinary state administrative disputes". The Administrative Court is also authorized to adjudicate "fictitious-positive decision disputes", namely disputes caused by legal events in the form of silence by government officials not issuing the requested decision, then the application is considered legally granted. Plaintiff (person or civil legal entity), before filing a lawsuit to the Administrative Court, it must first take an " Administrative Appeal ", it is determined if the authorized official does not respond (acts in silence) to Administrative Appeal, then "Administrative Appeal are deemed granted". Then there will be a juridical problem: "whether the dispute will be tried by PTUN as" Ordinary State Administrative Dispute "or as" Fictitious-Positive Decision Dispute? ".  The approach method used is Normative Juridical, qualitative normative analysis method, and grammatical and systematic interpretation method. Based on normative studies, it is not yet clear whether the legal event will become an "Ordinary TUN Dispute" or a "Fictitious-Positive Decision Dispute", because it cannot be classified into the two types of disputes. Certainty is needed, because the procedural law are very different between the two types of disputes. The Article 77 and Article 78 of the UU-AP need to be amended by adding the provisions in the next paragraph which stipulates: "if the applicant for an administrative objection or appeal wishes to submit to the Administrative Court, then the TUN dispute is resolved in the Ordinary Procedure or is resolved as a fictitious decision dispute- positive". It is very important to emphasize in the provisions of the article in order to realize the unity of the proceedings and legal certainty