bluuebunny/crossref_metadata_2025 huggingface dataset:
Relative Contributions of Different Lifestyle Factors to Health-Related Quality of Life in the Elderly
Authors: Zhang Xiaona , Xia Ruyi , Wang Shu , Xue Wei , Yang Jian , Sun Shuliu , Zhuang Guihua
Published: February 2018

Much of the previous literature has studied the relationship between individual lifestyle factors and the health-related quality of life (HRQOL). However, only a few studies combined them to explore their relative importance to the HRQOL in the elderly. This study assesses the HRQOL of the urban, rural, and institutionalized Chinese elderly and explores the relative contributions of different lifestyle factors to their HRQOL. The SF-36v2 Health Survey, the WHOQOL-OLD module, and the socio-demographic and lifestyle questionnaire were utilized in this study. Hierarchical regression was performed in order to analyze the results. The physical and mental component scores of the SF-36v2 survey were 47.05 ± 9.95 and 54.92 ± 9.92, respectively. The total score for the WHOQOL-OLD module was 73.01 ± 11.99, with institutionalized persons reporting lower scores. For the physical component of the elderly participants’ HRQOL, the R2 value changed the most (0.116) when exercise-and-labor-related factors were added in. For the mental component, sleep-related (0.054), and leisure-time-activity-related factors (0.053) caused the largest change of the R2 value. For the elderly-specific HRQOL, measured by the WHOQOL-OLD module, the leisure-time-activity-related factors caused the largest change in the R2 value (0.119), followed by exercise-and-labor-related factors (0.078). Heterogeneity was present among the three subgroups. In sum, compared with their community-dwelling counterparts, the HRQOL of institutionalized older people was relatively poor and different lifestyle factors contributed to the HRQOL differently.
Interactive Cumulative Burden Assessment: Engaging Stakeholders in an Adaptive, Participatory and Transdisciplinary Approach
Authors: Shrestha Rehana , Flacke Johannes , Martinez Javier , Van Maarseveen Martin
Published: February 2018

Cumulative burden assessment (CuBA) has the potential to inform planning and decision-making on health disparities related to multiple environmental burdens. However, scholars have raised concerns about the social complexity to be dealt with while conducting CuBA, suggesting that it should be addressed in an adaptive, participatory and transdisciplinary (APT) approach. APT calls for deliberation among stakeholders by engaging them in a process of social learning and knowledge co-production. We propose an interactive stakeholder-based approach that facilitates a science-based stakeholder dialogue as an interface for combining different knowledge domains and engendering social learning in CuBA processes. Our approach allows participants to interact with each other using a flexible and auditable CuBA model implemented within a shared workspace. In two workshops we explored the usefulness and practicality of the approach. Results show that stakeholders were enabled to deliberate on cumulative burdens collaboratively, to learn about the technical uncertainties and social challenges associated with CuBA, and to co-produce knowledge in a realm of both technical and societal challenges. The paper identifies potential benefits relevant for responding to social complexity in the CuBA and further recommends exploration of how our approach can enable or constraint social learning and knowledge co-production in CuBA processes under various institutional, social and political contexts.
Fat Mass Index and Body Mass Index Affect Peak Metabolic Equivalent Negatively during Exercise Test among Children and Adolescents in Taiwan
Authors: Tuan Shenghui , Su Hungtzu , Chen Yijen , Li Minhui , Tsai Yunjen , Yang Chunhan , Lin Kolong
Published: February 2018

Peak metabolic equivalent (MET) is the most reliable indicator of cardiorespiratory fitness (CRF). The aim of this study was to examine the association between CRF indicated by peak MET and body mass index (BMI) or fat mass index (FMI) in Taiwanese children and adolescents (C-A). Data of 638 C-A aged 10–18 that received symptom-limited treadmill exercise testing was analyzed. Anthropometry-body composition was measured by vector bioelectrical impedance analysis. BMI was defined as body weight (kg)/body height (m)2 and FMI was defined as fat mass (kg)/body height (m)2. BMI was grouped by Taiwanese obesity cut-off points. FMI Class-I was categorized by percentage of body fat. FMI Class-II used the reference values from Korean C-A. Excess adiposity was defined as (1) “overweight” and “obesity” by BMI, (2) greater than the sex- and age-specific 75th percentile of whole subjects by FMI Class-I, and (3) greater than 95th percentiles of reference value by FMI Class-II. Boys had significantly higher fat mass and FMI, and had more excess adiposity than girls (all p < 0.05). Both boys and girls with excess adiposity (by any definition) had lower MET at anaerobic threshold (AT MET) and peak MET (all p < 0.001). BMI and FMI were significantly negatively associated with both AT MET and peak MET significantly (all p < 0.001). FMI (95% CI: −0.411~−0.548) correlated with peak MET more than BMI (95% CI: −0.134~ −0.372) did. Excess adiposity affected CRF negatively. It is concluded that weight management should start early in childhood.
The Effect of a Leisure Time Physical Activity Intervention Delivered via a Workplace: 15-Month Follow-Up Study
Authors: Skogstad Marit , Lunde Lars-Kristian , Ulvestad Bente , Aass Hans , Clemm Thomas , Mamen Asgeir , Skare Øivind
Published: February 2018

In line with recommendations from both the World Health Organization and the European Union some employers encourage workplace health promotion through physical activity (PA) facilities and leisure time PA-initiatives. The current study describes a 15-month follow-up after an 8-week workplace delivered PA-initiative, investigates if improvements in cardiovascular risk status are sustainable, and elucidates differences according to educational level. One hundred and twenty one employees (43 women) were examined before and after the 8-week PA-initiative and 94 at the 15-month follow-up. PA-levels, blood pressure, resting heart rate, lipids, glycosylated hemoglobin (HbA1c), C-reactive protein (CRP), and selected markers of inflammation were registered at baseline, immediately after the 8-week PA-initiative, and 15 months after baseline. At the end of follow-up (15-month), PA-levels—increased during the 8-week intervention—had returned to baseline values. None of the five improvements in cardiovascular markers (total cholesterol, low density lipoprotein (LDL), P-selectin, CD40Ligand (CD40L) and Monocyte chemoattractant protein-1 (MCP-1)) seen at the 8-week follow-up were sustained. At the 15-month follow-up as compared to baseline HbA1c, CRP (log) and interleukin-6 (IL-6) were reduced by 0.06 mmol/L (95% CI −0.11,−0.01), 0.25 mg/L (95% CI −0.46,−0.04) and 0.39 pg/mL (95% CI −0.75, −0.04), respectively. At baseline, there were differences in cardiovascular risk factors comparing men with low versus high levels of education. No differences in changes in outcomes between these groups of men were found during follow-up. In this study highly educated men generally have lower levels of cardiovascular risk factors, but the effect of PA on such markers in this group do not differ from the effects seen in less educated men.
Do Authoritarian Governments Respond to Public Opinion on the Environment? Evidence from China
Authors: Tang Xiao , Chen Weiwei , Wu Tian
Published: February 2018

Given its serious impacts on the public’s health, air pollution in China is a matter of strong public concern, particularly in reference to malodorous waste gas. Petition letters related to atmospheric pollution accounted for about 40% of the total petition cases. However, scholarly views differ on whether the Chinese government responds to public opinion on the environment and seeks to improve its environmental governance behavior. For this study, data from national surveys on the public’s environmental satisfaction administered during the period 2011–2015 were analyzed to determine whether the public’s dissatisfaction with the state of the environment in a given year resulted in increased investments by provincial governments in pollution governance during the following year. The study’s findings revealed that governmental behavior in response to public opinion on the environment was selective within the field of environmental governance, with provincial governments being inclined to invest more in waste gas pollution control than in water pollution control. Furthermore, results from this study show that the Chinese government tends to put more efforts into the environmental field where it could more easily achieve short-term benefits.
Will Green CSR Enhance Innovation? A Perspective of Public Visibility and Firm Transparency
Authors: Wu Weiwei , Liu Yexin , Chin Tachia , Zhu Wenzhong
Published: February 2018

In response to the asking and requiring of stakeholders to be more environmentally responsible, firms must commit to green corporate social responsibility (CSR). Firms being green and responsible always can acquire intangible resources that are important for firm innovation. Given the scarcity of existing research addressing relevant issues in depth, this paper expands our understanding of green CSR by revealing its antecedent effects on firm innovation performance. We also include public visibility and firm transparency as contingency factors to explore the relationship between green CSR and firm innovation performance. Using data collected from publicly listed firms in China, we find that greater innovation performance is associated with an increase in firm green CSR, and the positive relationship between green CSR and innovation performance is moderated by public visibility and firm transparency. Based on the results, theoretical contributions and practical implications are outlined.
Expansion Analysis of Yangtze River Delta Urban Agglomeration Using DMSP/OLS Nighttime Light Imagery for 1993 to 2012
Authors: Lu Huimin , Zhang Meiliang , Sun Weiwei , Li Weiyue
Published: February 2018

Investigating the characteristics of urban expansion is helpful in managing the relationship between urbanization and the ecological and environmental issues related to sustainable development. The Defense Meteorological Satellite Program/Operational Line-scan System (DMSP/OLS) collects visible and near-infrared light from the Earth’s surface at night without moonlight. It generates effective time series data for mapping the dynamics of urban expansion. As a major urban agglomeration in the world, the Yangtze River Delta Urban Agglomeration (YRDUA) is an important intersection zone of both the “Belt and Road Initiative” and the “Yangtze River Economic Belt” in China. Therefore, this paper analyses urban expansion characteristics of the YRDUA for 1993–2012 from urban extents extracted from the DMSP/OLS for 1993, 1997, 2002, 2007, and 2012. First, calibration procedures are applied to DMSP/OLS data, including intercalibration, intra-annual composition, and inter-annual series correction procedures. Spatial extents are then extracted from the corrected DMSP/OLS data, and a threshold is determined via the spatial comparison method. Finally, three models are used to explore urban expansion characteristics of the YRDUA from expansion rates, expansion spatial patterns, and expansion evaluations. The results show that the urban expansion of the YRDUA occurred at an increasing rate from 1993–2007 and then declined after 2007 with the onset of the global financial crisis. The Suxichang and Ningbo metropolitan circles were seriously affected by the financial crisis, while the Hefei metropolitan circle was not. The urban expansion of the YRDUA moved from the northeast to the southwest over the 20-year period. Urban expansion involved internal infilling over the first 15 years and then evolved into external sprawl and suburbanization after 2007.
The Impact of Vitamin D in the Treatment of Essential Hypertension
Authors: Legarth Christian , Grimm Daniela , Wehland Markus , Bauer Johann , Krüger Marcus
Published: February 2018

The aim of this review is to investigate, whether there is a possible link between vitamin D supplementation and the reduction of blood pressure in hypertensive patients. The renin-angiotensin-aldosterone system is known for being deeply involved in cardiovascular tonus and blood pressure regulation. Hence, many of the pharmaceutical antihypertensive drugs inhibit this system. Interestingly, experimental studies in mice have indicated that vitamin D supplementation significantly lowers renin synthesis and blood pressure. It is conceivable that similar mechanisms may be found in the human organism. Regarding this, large-scale cross-sectional studies suggest the serum 25(OH)D-level to be inversely correlated to the prevalence of hypertension. However, randomized controlled trials (RCTs) have not found a clear association between vitamin D supplementation and improvements in hypertension. Nevertheless, the missing association of vitamin D and hypertension in clinical trials can be due to suboptimal study designs. There are hints that restoration of serum 25(OH)D levels during vitamin D therapy is essential to achieve possible beneficial cardiovascular effects. It is important to perform long-term trials with a short dose interval and a high bioavailability of supplementation. Taken together, more RCTs are required to further investigate if vitamin D can be beneficial for the reduction of blood pressure.
MicroRNAs as New Biomarkers for Diagnosis and Prognosis, and as Potential Therapeutic Targets in Acute Myeloid Leukemia
Authors: Trino Stefania , Lamorte Daniela , Caivano Antonella , Laurenzana Ilaria , Tagliaferri Daniela , Falco Geppino , Del Vecchio Luigi , Musto Pellegrino , De Luca Luciana
Published: February 2018

Acute myeloid leukemias (AML) are clonal disorders of hematopoietic progenitor cells which are characterized by relevant heterogeneity in terms of phenotypic, genotypic, and clinical features. Among the genetic aberrations that control disease development there are microRNAs (miRNAs). miRNAs are small non-coding RNAs that regulate, at post-transcriptional level, translation and stability of mRNAs. It is now established that deregulated miRNA expression is a prominent feature in AML. Functional studies have shown that miRNAs play an important role in AML pathogenesis and miRNA expression signatures are associated with chemotherapy response and clinical outcome. In this review we summarized miRNA signature in AML with different cytogenetic, molecular and clinical characteristics. Moreover, we reviewed the miRNA regulatory network in AML pathogenesis and we discussed the potential use of cellular and circulating miRNAs as biomarkers for diagnosis and prognosis and as therapeutic targets.
Neutrophils: Beneficial and Harmful Cells in Septic Arthritis
Authors: Boff Daiane , Crijns Helena , Teixeira Mauro , Amaral Flavio , Proost Paul
Published: February 2018

Septic arthritis is an inflammatory joint disease that is induced by pathogens such as Staphylococcus aureus. Infection of the joint triggers an acute inflammatory response directed by inflammatory mediators including microbial danger signals and cytokines and is accompanied by an influx of leukocytes. The recruitment of these inflammatory cells depends on gradients of chemoattractants including formylated peptides from the infectious agent or dying cells, host-derived leukotrienes, complement proteins and chemokines. Neutrophils are of major importance and play a dual role in the pathogenesis of septic arthritis. On the one hand, these leukocytes are indispensable in the first-line defense to kill invading pathogens in the early stage of disease. However, on the other hand, neutrophils act as mediators of tissue destruction. Since the elimination of inflammatory neutrophils from the site of inflammation is a prerequisite for resolution of the acute inflammatory response, the prolonged stay of these leukocytes at the inflammatory site can lead to irreversible damage to the infected joint, which is known as an important complication in septic arthritis patients. Thus, timely reduction of the recruitment of inflammatory neutrophils to infected joints may be an efficient therapy to reduce tissue damage in septic arthritis.
Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron
Authors: He Ao-Lei , Niu Shu-Qi , Zhao Qi , Li Yong-Sheng , Gou Jing-Yi , Gao Hui-Juan , Suo Sheng-Zhou , Zhang Jin-Lin
Published: February 2018

Drought and soil salinity reduce agricultural output worldwide. Plant-growth-promoting rhizobacteria (PGPR) can enhance plant growth and augment plant tolerance to biotic and abiotic stresses. Haloxylon ammodendron, a C4 perennial succulent xerohalophyte shrub with excellent drought and salt tolerance, is naturally distributed in the desert area of northwest China. In our previous work, a bacterium strain numbered as M30-35 was isolated from the rhizosphere of H. ammodendron in Tengger desert, Gansu province, northwest China. In current work, the effects of M30-35 inoculation on salt tolerance of perennial ryegrass were evaluated and its genome was sequenced to identify genes associated with plant growth promotion. Results showed that M30-35 significantly enhanced growth and salt tolerance of perennial ryegrass by increasing shoot fresh and dry weights, chlorophyll content, root volume, root activity, leaf catalase activity, soluble sugar and proline contents that contributed to reduced osmotic potential, tissue K+ content and K+/Na+ ratio, while decreasing malondialdehyde (MDA) content and relative electric conductivity (REC), especially under higher salinity. The genome of M30-35 contains 4421 protein encoding genes, 12 rRNA, 63 tRNA-encoding genes and four rRNA operons. M30-35 was initially classified as a new species in Pseudomonas and named as Pseudomonas sp. M30-35. Thirty-four genes showing homology to genes associated with PGPR traits and abiotic stress tolerance were identified in Pseudomonas sp. M30-35 genome, including 12 related to insoluble phosphorus solubilization, four to auxin biosynthesis, four to other process of growth promotion, seven to oxidative stress alleviation, four to salt and drought tolerance and three to cold and heat tolerance. Further study is needed to clarify the correlation between these genes from M30-35 and the salt stress alleviation of inoculated plants under salt stress. Overall, our research indicated that desert shrubs appear rich in PGPRs that can help important crops tolerate abiotic stress.
Design of Microscale Magnetic Tumbling Robots for Locomotion in Multiple Environments and Complex Terrains
Authors: Bi Chenghao , Guix Maria , Johnson Benjamin , Jing Wuming , Cappelleri David
Published: February 2018

This paper presents several variations of a microscale magnetic tumbling ( μ TUM) robot capable of traversing complex terrains in dry and wet environments. The robot is fabricated by photolithography techniques and consists of a polymeric body with two sections with embedded magnetic particles aligned at the ends and a middle nonmagnetic bridge section. The robot’s footprint dimensions are 400 μ m × 800 μ m. Different end geometries are used to test the optimal conditions for low adhesion and increased dynamic response to an actuating external rotating magnetic field. When subjected to a magnetic field as low as 7 mT in dry conditions, this magnetic microrobot is able to operate with a tumbling locomotion mode and translate with speeds of over 60 body lengths/s (48 mm/s) in dry environments and up to 17 body lengths/s (13.6 mm/s) in wet environments. Two different tumbling modes were observed and depend on the alignment of the magnetic particles. A technique was devised to measure the magnetic particle alignment angle relative to the robot’s geometry. Rotational frequency limits were observed experimentally, becoming more prohibitive as environment viscosity increases. The μ TUM’s performance was studied when traversing inclined planes (up to 60°), showing promising climbing capabilities in both dry and wet conditions. Maximum open loop straight-line trajectory errors of less than 4% and 2% of the traversal distance in the vertical and horizontal directions, respectively, for the μ TUM were observed. Full directional control of μ TUM was demonstrated through the traversal of a P-shaped trajectory. Additionally, successful locomotion of the optimized μ TUM design over complex terrains was also achieved. By implementing machine vision control and/or embedding of payloads in the middle section of the robot, it is possible in the future to upgrade the current design with computer-optimized mobility through multiple environments and the ability to perform drug delivery tasks for biomedical applications.
High-Performance MIM Capacitors for a Secondary Power Supply Application
Authors: Mu Jiliang , Chou Xiujian , Ma Zongmin , He Jian , Xiong Jijun
Published: February 2018

Microstructure is important to the development of energy devices with high performance. In this work, a three-dimensional Si-based metal-insulator-metal (MIM) capacitor has been reported, which is fabricated by microelectromechanical systems (MEMS) technology. Area enlargement is achieved by forming deep trenches in a silicon substrate using the deep reactive ion etching method. The results indicate that an area of 2.45 × 103 mm2 can be realized in the deep trench structure with a high aspect ratio of 30:1. Subsequently, a dielectric Al2O3 layer and electrode W/TiN layers are deposited by atomic layer deposition. The obtained capacitor has superior performance, such as a high breakdown voltage (34.1 V), a moderate energy density (≥1.23 mJ/cm2) per unit planar area, a high breakdown electric field (6.1 ± 0.1 MV/cm), a low leakage current (10−7 A/cm2 at 22.5 V), and a low quadratic voltage coefficient of capacitance (VCC) (≤63.1 ppm/V2). In addition, the device’s performance has been theoretically examined. The results show that the high energy supply and small leakage current can be attributed to the Poole–Frenkel emission in the high-field region and the trap-assisted tunneling in the low-field region. The reported capacitor has potential application as a secondary power supply.
Characterization of the Lime Mortars of the Rui Barbosa House Museum in Rio De Janeiro, Brazil
Authors: Pereira da Silva Dalto Daniele , Carlos da Conceição Ribeiro Roberto , Cavalcanti Rebecchi de Moura Luanna
Published: February 2018

The aim of this research is to characterize the mortars of the Rui Barbosa House Museum, built in 1850, in the city of Rio de Janeiro, Brazil, using X-ray diffraction (XRD), gel-CSH determination, Fourier Transform Infrared spectrometry (FTIR), Wavelength-Dispersive X-ray Fluorescence spectrometry (WDXRF), Thermo gravimetric analysis (TG-DTG), and granulometric analysis. Also, petrographic and physical characteristics such as porosity, water absorption, and apparent density of the mortars were used to identify the raw materials employed in their preparation. Mineralogical associations found are similar (quartz, calcite, kaolinite, microcline, muscovite, and albite) for the majority of samples and only vary in their proportions; the most prominent phases are quartz and calcite. The collected data, indicating that the binder is hydrated lime, lead us to conclude that the analyzed samples are lime mortars, discarding the possibility of cement as the binder material. Two samples, located on the corner of the building, which underwent repairs, present a differentiated behavior, with high levels of dolomite and the presence of pozzolanic material, with greater water absorption and higher density, indicating the use of a different mortar type. In addition, it was verified that the WDXRF and XRD methods are able to determine the trace element composition with comparable precision with respect to the methodological approach proposed by Teutonico (1988). In addition, it has been verified that the major pollutant causing degradation of building are sulfur emitted by the vehicles and saline spray.
Cell Migration Related to MDR—Another Impediment to Effective Chemotherapy?
Authors: Kryczka Jakub , Boncela Joanna
Published: February 2018

Multidrug resistance, mediated by members of the ATP-binding cassette (ABC) proteins superfamily, has become one of the biggest obstacles in conquering tumour progression. If the chemotherapy outcome is considered successful, when the primary tumour volume is decreased or completely abolished, modulation of ABC proteins activity is one of the best methods to overcome drug resistance. However, if a positive outcome is represented by no metastasis or, at least, elongation of remission-free time, then the positive effect of ABC proteins inhibition should be compared with the several side effects it causes, which may inflict cancer progression and decrease overall patient health. Clinical trials conducted thus far have shown that the tested ABC modulators add limited or no benefits to cancer patients, as some of them are merely toxic and others induce unwanted drug–drug interactions. Moreover, the inhibition of certain ABC members has been recently indicated as potentially responsible for increased fibroblasts migration. A better understanding of the complex role of ABC proteins in relation to cancer progression may offer novel strategies in cancer therapy.
The Fungal Metabolite Eurochevalierine, a Sequiterpene Alkaloid, Displays Anti-Cancer Properties through Selective Sirtuin 1/2 Inhibition
Authors: Schnekenburger Michael , Mathieu Véronique , Lefranc Florence , Jang Jun Young , Masi Marco , Kijjoa Anake , Evidente Antonio , Kim Hyun-Jung , Kiss Robert , Dicato Mario , Han Byung Woo , Diederich Marc
Published: February 2018

NAD+-dependent histone deacetylases (sirtuins) are implicated in cellular processes such as proliferation, DNA repair, and apoptosis by regulating gene expression and the functions of numerous proteins. Due to their key role in cells, the discovery of small molecule sirtuin modulators has been of significant interest for diverse therapeutic applications. In particular, it has been shown that inhibition of sirtuin 1 and 2 activities is beneficial for cancer treatment. Here, we demonstrate that the fungal metabolite eurochevalierine from the fungus Neosartorya pseudofischeri inhibits sirtuin 1 and 2 activities (IC50 about 10 µM) without affecting sirtuin 3 activity. The binding modes of the eurochevalierine for sirtuin 1 and 2 have been identified through computational docking analyses. Accordingly, this sequiterpene alkaloid induces histone H4 and α-tubulin acetylation in various cancer cell models in which it induces strong cytostatic effects without affecting significantly the viability of healthy PBMCs. Importantly, eurochevalierine targets preferentially cancer cell proliferation (selectivity factor ≫ 7), as normal human primary CD34+ stem/progenitor cells were less affected by the treatment. Finally, eurochevalierine displays suitable drug-likeness parameters and therefore represent a promising scaffold for lead molecule optimization to study the mechanism and biological roles of sirtuins and potentially a basis for development into therapeutics.
Polymer-Based Nanocarriers for Co-Delivery and Combination of Diverse Therapies against Cancers
Authors: Yan Guowen , Li Aihua , Zhang Aitang , Sun Yong , Liu Jingquan
Published: February 2018

Cancer gives rise to an enormous number of deaths worldwide nowadays. Therefore, it is in urgent need to develop new therapies, among which combined therapies including photothermal therapy (PTT) and chemotherapy (CHT) using polymer-based nanocarriers have attracted enormous interest due to the significantly enhanced efficacy and great progress has been made so far. The preparation of such nanocarriers is a comprehensive task involving the cooperation of nanomaterial science and biomedicine science. In this review, we try to introduce and analyze the structure, preparation and synergistic therapeutic effect of various polymer-based nanocarriers composed of anti-tumor drugs, nano-sized photothermal materials and other possible parts. Our effort may bring benefit to future exploration and potential applications of similar nanocarriers.
Antibacterial Effects of Chitosan/Cationic Peptide Nanoparticles
Authors: Tamara Frans , Lin Chi , Mi Fwu-Long , Ho Yi-Cheng
Published: February 2018

This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and antibacterial activities of the hybrid nanoparticles were investigated. The antibacterial experiments demonstrated that the addition of protamine (125 µg/mL) in the hybrid nanoparticles (500 µg/mL chitosan and 166.67 µg/mL TPP) improved the antimicrobial specificity with the minimum inhibitory concentration (MIC) value of 31.25 µg/mL towards Escherichia coli (E. coli), while the MIC value was higher than 250 µg/mL towards Bacillus cereus. The chitosan/protamine hybrid nanoparticles induced the formation of biofilm-like structure in B. cereus and non-motile-like structure in E. coli. The detection of bacterial cell ruptures showed that the inclusion of protamine in the hybrid nanoparticles caused different membrane permeability compared to chitosan nanoparticles and chitosan alone. The chitosan/protamine nanoparticles also exhibited lower binding affinity towards B. cereus than E. coli. The results suggested that the hybridization of chitosan with protamine improved the antibacterial activity of chitosan nanoparticles towards pathogenic E. coli, but the inhibitory effect against probiotic B. cereus was significantly reduced.
Unravelling the Long Non-Coding RNA Profile of Undifferentiated Large Cell Lung Carcinoma
Authors: Shukla Sudhanshu
Published: February 2018

Undifferentiated large cell lung carcinoma (LCLC) accounts for 2.9–9% of total lung cancers. Recently, RNA-seq based studies have revealed major genomic aberrations in LCLC. In this study, we aim to identify long non-coding RNAs (LncRNAs) expression pattern specific to LCLC. The RNA-seq profile of LCLC and other non-small cell lung carcinoma (NSCLC) was downloaded from Gene Expression Omnibus (GEO) and analyzed. Using 10 LCLC samples, we found that 18% of all the annotated LncRNAs are expressed in LCLC samples. Among 1794 expressed LncRNAs, 11 were overexpressed and 14 were downregulated in LCLC compared to normal samples. Based on receiver operating characteristic (ROC) analysis, we showed that the top five differentially expressed LncRNAs were able to differentiate between LCLC and normal samples with high sensitivity and specificity. Guilt by association analysis using genes correlating with differentially expressed LncRNAs identified several cancer-associated pathways, suggesting the role of these deregulated LncRNA in LCLC biology. We also identified the LncRNA differentially expressed in LCLC compared to lung squamous carcinoma (LUSC) and Lung-adenocarcinoma (LUAD). We found that LCLC sample showed more deregulated LncRNA in LUSC than LUAD. Interestingly, LCLC had more downregulated LncRNA compared to LUAD and LUSC. Our study provides novel insight into LncRNA deregulation in LCLC. This study also finds tools to diagnose LCLC and differentiate LCLC with other Non-Small Cell Lung Cancer.
An Active Learning Activity to Reinforce the Design Components of the Corticosteroids
Authors: Slauson Stephen , Mandela Prashant
Published: February 2018

Despite the popularity of active learning applications over the past few decades, few activities have been reported for the field of medicinal chemistry. The purpose of this study is to report a new active learning activity, describe participant contributions, and examine participant performance on the assessment questions mapped to the objective covered by the activity. In this particular activity, students are asked to design two novel corticosteroids as a group (6–8 students per group) based on the design characteristics of marketed corticosteroids covered in lecture coupled with their pharmaceutics knowledge from the previous semester and then defend their design to the class through an interactive presentation model. Although class performance on the objective mapped to this material on the assessment did not reach statistical significance, use of this activity has allowed fruitful discussion of misunderstood concepts and facilitated multiple changes to the lecture presentation. As pharmacy schools continue to emphasize alternative learning pedagogies, publication of previously implemented activities demonstrating their use will help others apply similar methodologies.
An Investigation and Comparison of the Blending of LDPE and PP with Different Intrinsic Viscosities of PET
Authors: Chen Shi-Chang , Zhang Li-Hao , Zhang Guo , Zhong Guo-Cai , Li Jian , Zhang Xian-Ming , Chen Wen-Xing
Published: February 2018

The blending of aliphatic polyolefins and aromatic poly(ethylene terephthalate) (PET) based on different intrinsic viscosities (IV) was conducted in a torque rheometer. The comparison of blend components in terms of low density polythene (LDPE) and polypropylene (PP) in blending with PET was investigated, and the effects of the IV and proportion of PET on polymer blends are discussed in detail. Polymer blends with or without compatibilizer were examined by using a differential scanning calorimeter, thermogravimetric analyzer, rotary rheometer, field-emission scanning electron microscopy and a universal testing machine. It was found that the blending led to an increase in processability and a decrease in thermal stability for blends. The morphological analysis revealed that the incompatibility of blends was aggravated by a higher IV of PET, while this situation could be improved by the addition of compatibilizer. Results showed that there was an opposite effect for the tensile strength and the elongation at break of the polymer blend in the presence of a compatibilizer, wherein the influence of IV of PET was complicated.
Evolution of Filament-Shaped Porous Structure in Polycarbonate by Stretching under Carbon Dioxide
Authors: Taguchi Tomoaki , Hatakeyama Tomoe , Miike Ramu , Saito Hiromu
Published: February 2018

We found that a filament-shaped porous structure with periodic distance was obtained in polycarbonate for optical disk grade (OD-PC) film by stretching under compressed carbon dioxide (CO2). The evolution of the characteristic porous structure was investigated by in situ observation during the stretching under compressed CO2 and the optical microscopic observation of the stretched specimen. The voids were obtained under high CO2 pressure as in the case of elevated temperature, suggesting that the evolution of the voids was caused by crazing due to chain disentanglement by accelerated molecular motion owing to the plasticization effect of CO2. The filament-shaped voids were initiated at around the yielding point and increased continuously by nucleation in the matrix around the surface of the pre-existing voids. The shape of the voids did not change to an ellipsoidal one during stretching due to suppression of the craze opening by the hydrostatic pressure effect. The stretching of the CO2-absorbed depressurized OD-PC revealed that the initiation of the voids was not only caused by the plasticization effect, but the hydrostatic pressure effect was also required.
In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets
Authors: Segura Víctor , Valero M. Luz , Cantero Laura , Muñoz Javier , Zarzuela Eduardo , García Fernando , Aloria Kerman , Beaskoetxea Javier , Arizmendi Jesús M. , Navajas Rosana , Paradela Alberto , Díez Paula , Dégano Rosa Mª , Fuentes Manuel , Orfao Alberto , García Montero Andrés , Garin-Muga Alba , Corrales Fernando J. , Sánchez del Pino Manuel M.
Published: February 2018

Monocytes are bone marrow-derived leukocytes that are part of the innate immune system. Monocytes are divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16. These cells are key players in the inflammation process underlying the mechanism of many diseases. Thus, the molecular characterization of these cells may provide very useful information for understanding their biology in health and disease. We performed a multicentric proteomic study with pure classical and non-classical populations derived from 12 healthy donors. The robust workflow used provided reproducible results among the five participating laboratories. Over 5000 proteins were identified, and about half of them were quantified using a spectral counting approach. The results represent the protein abundance catalogue of pure classical and enriched non-classical blood peripheral monocytes, and could serve as a reference dataset of the healthy population. The functional analysis of the differences between cell subsets supports the consensus roles assigned to human monocytes.
Sustainable Airport Waste Management: The Case of Kansai International Airport
Authors: Baxter Glenn , Srisaeng Panarat , Wild Graham
Published: February 2018

The global air transport industry is predicted to continue its rapid growth. A by-product of air transport operations, however, is the substantial volumes of waste generated at airports. To mitigate the environmental impact of waste and to comply with regulatory requirements, airports are increasingly implementing sustainable waste management policies and systems. Using an in-depth case study research design, this study has examined waste management at Kansai International Airport from 2002 to 2015. Throughout its history the airport has implemented world best practices to achieve its goal of being an eco-friendly airport. The qualitative data gathered for the study were analysed using document analysis. The quantitative data were analysed using t-tests. Statistically significant results were found in the reduction in waste per passenger and aircraft movement (for total waste, incinerated waste, and landfill waste). In addition, a statistically significant increase in the proportion of waste recycled, and a decrease in the proportion of waste sent to landfill was observed. As such, quantitatively speaking, Kansai International Airport has shown significant waste management improvements. The study concludes that Kansai Airport’s waste management approaches and policies can be transferred to other airport facilities. This would greatly improve sustainability across airports, globally.
Evil and Human Suffering in Islamic Thought—Towards a Mystical Theodicy
Authors: Rouzati Nasrin
Published: February 2018

This paper sheds light on the treatment of the ‘problem of evil’ and human suffering from an Islamic perspective. I begin by providing an overview of the term ‘evil’ in the Qur’an to highlight its multidimensional meaning and to demonstrate the overall portrait of this notion as it is presented in the Islamic revelation through the narrative of the prophet Job. Having established a Qur’anic framework, I will then provide a brief historical overview of the formation of philosophical and theological debates surrounding “good” and “bad/evil” and the origination of Muslim theodicean thought. This will lead us to Ghazālian theodicy and the famous dictum of the “best of all possible worlds” by one of the most influential scholars of Islamic thought, Abu Ḥāmid Ghazālī. The final section of this paper will explore the Sufi/ mystical tradition of Islam through the teachings of one of the most distinguished mystics of Islam, Jalāl al-Dīn Rūmī. The conclusion of the paper will attempt to bring about a new understanding of how the so-called “problem of evil” is not presented in Islam as a problem but rather as an instrument in the actualization of God’s plan, which is intertwined with human experiences in this world—an experience that is necessary for man’s spiritual development.
Regional Inequality in China Based on NPP-VIIRS Night-Time Light Imagery
Authors: Wu Rongwei , Yang Degang , Dong Jiefang , Zhang Lu , Xia Fuqiang
Published: February 2018

Regional economic inequality is a persistent problem for all nations. Meanwhile, satellite-derived night-time light (NTL) data have been extensively used as an efficient proxy measure for economic activity. This study firstly proposes a new method for correction of the NTL data derived from the Visible Infrared Imaging Radiometer Suite (VIIRS) aboard the Suomi National Polar-orbiting Partnership (Suomi-NPP) satellite and then applies the corrected NTL data to estimate gross domestic product (GDP) at a multi-scale level in China from 2014 to 2017. Secondly, incorporating the two-stage nested Theil decomposition method, multi-scale level regional inequalities are investigated. Finally, by using scatter plots, this paper identifies the relationship between the regional inequality and the level of economic development. The results indicate that: (1) after correction, the NPP-VIIRS NTL data show a statistically positive correlation with GDP, which proves that our correction method is scientifically effective; (2) from 2014 to 2017, overall inequality, within-province inequality, and between-region inequality all declined, However, between-province inequality increased slightly. As for the contributions to overall regional inequality, the within-province inequality was the highest, while the between-province inequality was the lowest; (3) further analysis of within-province inequality reveals that economic inequalities in coastal provinces in China are smaller than in inland provinces; (4) China’s economic development plays an important role in affecting regional inequality, and the extent of influence of economic development on regional inequality is varied across provinces.
Analysis of Flow Cytometric Fluorescence Lifetime with Time-Delay Estimation of Pulse Signals
Authors: Zhu Lianqing , Zhang Wenchang , Dong Mingli , Lou Xiaoping
Published: February 2018

The measurement of fluorescence lifetimes emerged in flow cytometry because it is not impacted by the non-linearity, which occurs in fluorescence intensity measurements. However, this significantly increases the cost and complexity of a traditional flow cytometer. This work reports a simple method of fluorescence lifetime measurement of a flow cytometer based on the cytometric fluorescence pulse time-delay estimation and hardware time-delay calibration. The modified chirp Z-transform (MCZT) algorithm, combined with the algorithm of fine interpolation of correlation peak (FICP), is applied to improve the temporal resolution of the cross-correlation function of the scattering and fluorescence signals, which in turn improves the time-delay estimation accuracy. The estimation accuracy is verified by Gauss fitting. Cells that were labeled simultaneously with three-color reagents are measured; the statistical results of 5000 cells are compared with reference values and are verified with the pulse width variation. The results show the potential of fluorescence lifetime measurements in the traditional flow cytometer.
Speed Bump Detection Using Accelerometric Features: A Genetic Algorithm Approach
Authors: Celaya-Padilla Jose , Galván-Tejada Carlos , López-Monteagudo F. , Alonso-González O. , Moreno-Báez Arturo , Martínez-Torteya Antonio , Galván-Tejada Jorge , Arceo-Olague Jose , Luna-García Huizilopoztli , Gamboa-Rosales Hamurabi
Published: February 2018

Among the current challenges of the Smart City, traffic management and maintenance are of utmost importance. Road surface monitoring is currently performed by humans, but the road surface condition is one of the main indicators of road quality, and it may drastically affect fuel consumption and the safety of both drivers and pedestrians. Abnormalities in the road, such as manholes and potholes, can cause accidents when not identified by the drivers. Furthermore, human-induced abnormalities, such as speed bumps, could also cause accidents. In addition, while said obstacles ought to be signalized according to specific road regulation, they are not always correctly labeled. Therefore, we developed a novel method for the detection of road abnormalities (i.e., speed bumps). This method makes use of a gyro, an accelerometer, and a GPS sensor mounted in a car. After having the vehicle cruise through several streets, data is retrieved from the sensors. Then, using a cross-validation strategy, a genetic algorithm is used to find a logistic model that accurately detects road abnormalities. The proposed model had an accuracy of 0.9714 in a blind evaluation, with a false positive rate smaller than 0.018, and an area under the receiver operating characteristic curve of 0.9784. This methodology has the potential to detect speed bumps in quasi real-time conditions, and can be used to construct a real-time surface monitoring system.
SNDR Limits of Oscillator-Based Sensor Readout Circuits
Authors: Cardes Fernando , Quintero Andres , Gutierrez Eric , Buffa Cesare , Wiesbauer Andreas , Hernandez Luis
Published: February 2018

This paper analyzes the influence of phase noise and distortion on the performance of oscillator-based sensor data acquisition systems. Circuit noise inherent to the oscillator circuit manifests as phase noise and limits the SNR. Moreover, oscillator nonlinearity generates distortion for large input signals. Phase noise analysis of oscillators is well known in the literature, but the relationship between phase noise and the SNR of an oscillator-based sensor is not straightforward. This paper proposes a model to estimate the influence of phase noise in the performance of an oscillator-based system by reflecting the phase noise to the oscillator input. The proposed model is based on periodic steady-state analysis tools to predict the SNR of the oscillator. The accuracy of this model has been validated by both simulation and experiment in a 130 nm CMOS prototype. We also propose a method to estimate the SNDR and the dynamic range of an oscillator-based readout circuit that improves by more than one order of magnitude the simulation time compared to standard time domain simulations. This speed up enables the optimization and verification of this kind of systems with iterative algorithms.
Visual Estimation of Bacterial Growth Level in Microfluidic Culture Systems
Authors: Kim Kyukwang , Kim Seunggyu , Jeon Jessie
Published: February 2018

Microfluidic devices are an emerging platform for a variety of experiments involving bacterial cell culture, and has advantages including cost and convenience. One inevitable step during bacterial cell culture is the measurement of cell concentration in the channel. The optical density measurement technique is generally used for bacterial growth estimation, but it is not applicable to microfluidic devices due to the small sample volumes in microfluidics. Alternately, cell counting or colony-forming unit methods may be applied, but these do not work in situ; nor do these methods show measurement results immediately. To this end, we present a new vision-based method to estimate the growth level of the bacteria in microfluidic channels. We use Fast Fourier transform (FFT) to detect the frequency level change of the microscopic image, focusing on the fact that the microscopic image becomes rough as the number of cells in the field of view increases, adding high frequencies to the spectrum of the image. Two types of microfluidic devices are used to culture bacteria in liquid and agar gel medium, and time-lapsed images are captured. The images obtained are analyzed using FFT, resulting in an increase in high-frequency noise proportional to the time passed. Furthermore, we apply the developed method in the microfluidic antibiotics susceptibility test by recognizing the regional concentration change of the bacteria that are cultured in the antibiotics gradient. Finally, a deep learning-based data regression is performed on the data obtained by the proposed vision-based method for robust reporting of data.