bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Biochar Diet Supplementation on Chicken Broilers Performance, NH3 and Odor Emissions and Meat Consumer Acceptance
Authors: Kalus Kajetan , Konkol Damian , Korczyński Mariusz , Koziel Jacek A. , Opaliński Sebastian
Published: September 2020

The aim of this research was to evaluate the effect of biochar diet supplementation for broiler chickens on (1) ammonia and odor emissions from manure, (2) feed conversion ratio and daily weight gain, and (3) selected meat quality and sensory parameters. Beechwood biochar (BC, 2 and 4%) and BC–glycerin–aluminosilicates mix (BCM, 3 and 6%) were tested as dietary additives. A total of 750 chicken broilers (Ross 308) were divided into five dietary groups with five replicates per group (n = 5, 30 birds in each replicate) and reared on a littered floor for 5 weeks. Both feed additives showed a significant reduction of ammonia emissions by up to 17%, while the reduction of odor emissions was not statistically significant. The feed conversion ratio increased by 8% for the highest concentration of the mixture. The change of the treated broilers’ average body weight ranged in the last week of the experiment from 0 to −7%, with the most negative effect for the highest dose of the mixture. Sensory analysis of the sous-vide cooked breasts showed no significant differences.
Phages as a Cohesive Prophylactic and Therapeutic Approach in Aquaculture Systems
Authors: Żaczek Maciej , Weber-Dąbrowska Beata , Górski Andrzej
Published: September 2020

Facing antibiotic resistance has provoked a continuously growing focus on phage therapy. Although the greatest emphasis has always been placed on phage treatment in humans, behind phage application lies a complex approach that can be usefully adopted by the food industry, from hatcheries and croplands to ready-to-eat products. Such diverse businesses require an efficient method for combating highly pathogenic bacteria since antibiotic resistance concerns every aspect of human life. Despite the vast abundance of phages on Earth, the aquatic environment has been considered their most natural habitat. Water favors multidirectional Brownian motion and increases the possibility of contact between phage particles and their bacterial hosts. As the global production of aquatic organisms has rapidly grown over the past decades, phage treatment of bacterial infections seems to be an obvious and promising solution in this market sector. Pathogenic bacteria, such as Aeromonas and Vibrio, have already proved to be responsible for mass mortalities in aquatic systems, resulting in economic losses. The main objective of this work is to summarize, from a scientific and industry perspective, the recent data regarding phage application in the form of targeted probiotics and therapeutic agents in aquaculture niches.
Vancomycin-Resistant Enterococci (VRE) in Nigeria: The First Systematic Review and Meta-Analysis
Authors: Wada Yusuf , Harun Azian Binti , Yean Chan Yean , Zaidah Abdul Rahman
Published: September 2020

Vancomycin-Resistant Enterococci (VRE) are on the rise worldwide. Here, we report the first prevalence of VRE in Nigeria using systematic review and meta-analysis. International databases MedLib, PubMed, International Scientific Indexing (ISI), Web of Science, Scopus, Google Scholar, and African journals online (AJOL) were searched. Information was extracted by two independent reviewers, and results were reviewed by the third. Two reviewers independently assessed the study quality using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) checklist. OpenMeta analyst was used. The random effect was used, and publication bias was assessed using a funnel plot. Between-study heterogeneity was assessed, and the sources were analysed using the leave-one-out meta-analysis, subgroup analysis, and meta-regression. Nineteen studies met the eligibility criteria and were added to the final meta-analysis, and the study period was from 2009–2018. Of the 2552 isolates tested, 349 were VRE, and E. faecalis was reported the most. The pooled prevalence of VRE in Nigeria was estimated at 25.3% (95% CI; 19.8–30.8%; I2 = 96.26%; p < 0.001). Between-study variability was high (t2 = 0.011; heterogeneity I2 = 96.26% with heterogeneity chi-square (Q) = 480.667, degrees of freedom (df) = 18, and p = 0.001). The funnel plot showed no publication bias, and the leave-one-out forest plot did not affect the pooled prevalence. The South-East region had a moderate heterogeneity though not significant (I2 = 51.15%, p = 0.129). Meta-regression showed that all the variables listed contributed to the heterogeneity except for the animal isolate source (p = 0.188) and studies that were done in 2013 (p = 0.219). Adherence to proper and accurate antimicrobial usage, comprehensive testing, and continuous surveillance of VRE are required.
Olive Leaves and Hibiscus Flowers Extracts-Based Preparation Protect Brain from Oxidative Stress-Induced Injury
Authors: Chiaino Elda , Micucci Matteo , Cosconati Sandro , Novellino Ettore , Budriesi Roberta , Chiarini Alberto , Frosini Maria
Published: September 2020

Oxidative stress (OS) arising from tissue redox imbalance, critically contributes to the development of neurodegenerative disorders. Thus, natural compounds, owing to their antioxidant properties, have promising therapeutic potential. Pres phytum (PRES) is a nutraceutical product composed of leaves- and flowers-extracts of Olea europaea L. and Hibiscus sabdariffa L., respectively, the composition of which has been characterized by HPLC coupled to a UV-Vis and QqQ-Ms detector. As PRES possess antioxidant, antiapoptotic and anti-inflammatory properties, the aim of this study was to assess its neuroprotective effects in human neuroblastoma SH-SY5Y cells and in rat brain slices subjected to OS. PRES (1–50 µg/mL) reverted the decrease in viability as well as the increase in sub-diploid-, DAPI-and annexin V-positive-cells, reduced ROS formation, recovered the mitochondrial potential and caspase-3 and 9 activity changes caused by OS. PRES (50–100 µg/mL) neuroprotective effects occurred also in rat brain slices subjected to H2O2 challenge. Finally, as the neuroprotective potential of PRES is strictly related to its penetration into the brain and a relatively good pharmacokinetic profile, an in-silico prediction of its components drug-like properties was carried out. The present results suggest the possibility of PRES as a nutraceutical, which could help in preventing neurodegenerative diseases.
Dietary Intervention Accelerates NASH Resolution Depending on Inflammatory Status with Minor Additive Effects on Hepatic Injury by Vitamin E Supplementation
Authors: Klaebel Julie Hviid , Rakipovski Günaj , Andersen Birgitte , Lykkesfeldt Jens , Tveden-Nyborg Pernille
Published: September 2020

Despite the lack of effective pharmacotherapy against nonalcoholic steatohepatitis (NASH) and liver fibrosis, vitamin E (vitE) supplementation and lifestyle modifications are recommended for the management of NASH due to promising clinical results. We recently reported a positive effect of supplementation with 800 IU vitE and atorvastatin on NASH resolution in guinea pigs. In the present study, we investigated the effect of high-dose vitE therapy combined with dietary intervention against progressive NASH and advanced fibrosis in the guinea pig model. Sixty-six guinea pigs received either high-fat (HF) or standard guinea pig chow diet (Control) for 25 weeks. Prior to eight weeks of intervention, HF animals were allocated into groups; dietary intervention (Chow) or dietary intervention with 2000 IU/d vitE supplementation (CvitE). Both Chow and CvitE reduced dyslipidemia, hepatic lipid accumulation and liver weight (p < 0.05), while CvitE further decreased hepatocellular ballooning (p < 0.05). Subanalyses of individual responses within intervention groups showed significant correlation between the hepatic hallmarks of NASH and lipid accumulation vs. inflammatory state (p < 0.05). Collectively, our results indicate that individual differences in sensitivity towards intervention and inflammatory status determine the potential beneficial effect of dietary intervention and high-dose vitE supplementation. Moreover, the study suggests that inflammation is a primary target in NASH treatment.
Natural Nrf2 Modulators for Skin Protection
Authors: Boo Yong Chool
Published: September 2020

Since the discovery of antioxidant responsive elements (ARE), which are commonly found in the promoter of the Phase II metabolism/antioxidant enzymes, and nuclear factor erythroid 2-related factor 2 (Nrf2), the transcription factor that binds to ARE, the study conducted in this field has expanded remarkably over the decades, and the Nrf2-mediated pathway is now recognized to occupy a central position in cell defense mechanisms. Induction of the Phase II metabolism/antioxidant enzymes through direct activation of Nrf2 can be a promising strategy for preventing degenerative diseases in general, but a dark side of this strategy should be considered, as Nrf2 activation can enhance the survival of cancer cells. In this review, we discuss the historical discovery of Nrf2 and the regulatory mechanism of the Nrf2-mediated pathway, focusing on the interacting proteins and post-translational modifications. In addition, we discuss the latest studies that examined various natural Nrf2 modulators for the protective roles in the skin, in consideration of their dermatological and cosmetic applications. Studies are reviewed in the order of time of research as much as possible, to help understand how and why such studies were conducted under the circumstances of that time. We hope that this review can serve as a steppingstone in conducting more advanced research by providing a scientific basis for researchers newly entering this field.
Experimental Study of Artificial Ground Freezing by Natural Cold Gas Injection
Authors: Liu Zhao , Sun Youhong , Wang Bingge , Li Qiang
Published: September 2020

The application of conventional artificial ground freezing (AGF) has two disadvantages: low freezing rate and small frozen range. In this study, a new method with natural cold gas injection was proposed, whereby the shallow soils and water can be frozen rapidly due to the effect of the heat convection. Cold gas from −15 °C to −10 °C, in the winter of northeast China, was injected into the laboratory-scale sand pipe; evolution of the induced frozen front and water migration were studied, and then, the feasibility of the new method was analyzed. According to the evolution of the induced frozen front, the freezing process was divided into an initial cooling stage, phase transition stage, and subcooled stage. The results showed that the increase of initial water content at the beginning of the experiments had little effect on the time required for completing the initial cooling stage, while the time required for the phase transition would increase in nearly the same proportion. In addition, the increase of the cold gas flow rate could not only strengthen the cooling rate of the initial cooling stage but also shorten the phase transition time; thereby, the freezing rate was increased. The freezing rate could reach 0.18–0.61 cm/min in the direction of cold gas flow, and compared to the conventional AGF (months are required for approximately 1 m), the freezing efficiency was greatly improved.
Simulation-Based Generation of Representative and Valid Training Data for Acoustic Resonance Testing
Authors: Heinrich Matthias , Rabe Ute , Valeske Bernd
Published: September 2020

Analyzing eigenfrequencies of serial parts by acoustic resonance testing enables an efficient nondestructive assessment of component quality or structural state. Usually, each application is based on experimentally acquired training data, which represent the typical natural vibration behavior of the component type to be inspected. From the training data, suitable test characteristics are identified according to the inspection objective. The experimental collection of training data, which involves selecting and characterizing numerous representing parts, is often associated with a great amount of effort. Instead, this work focuses on a simulation-based generation of synthetic training data. Within an application example, the eigenfrequencies of a set of virtual parts were calculated with FEM as a function of geometry. The resulting simulation values were adapted using empirical correction factors, which were derived from both calculated and measured eigenfrequencies of machine-made reference parts. The simulation-based data were finally used to form linear regression models within a training procedure. These models enabled the precise estimation of geometric dimensions of further machine-made parts using their measured eigenfrequencies as input data. The novel approach, which requires the experimental characterization of only a few real parts, can thus significantly reduce the effort associated with efficient and reliable acoustic resonance testing.
The Control of Volume Expansion and Porosity in Carbon Block by Carbon Black (CB) Addition for Increasing Thermal Conductivity
Authors: Kim Min Il , Cho Jong Hoon , Bai Byong Chol , Im Ji Sun
Published: September 2020

The graphite block as a phase change materials (PCMs) was manufactured by graphitization of a carbon block. Carbon blocks were prepared by filler (cokes or graphite) and binder (pitch). The binder-coated filler was thermally treated for carbonization. The gases generated from the evaporation of low molecular weight components in the binder pitch during the carbonization process were not released to the outside. Consequently, porosity and volume expansion were increased in artificial graphite, and thereby the thermal conductivity decreased. In this study, to prevent the decrease of thermal conductivity in the artificial graphite due to the disadvantages of binder pitch, the carbon block was prepared by the addition of carbon black, which can absorb low molecular weight compounds and release the generated gas. The properties of the prepared carbon blocks were analyzed by SEM, TGA, and thermal conductivity. The addition of carbon black (CB) decreased the porosity and volume expansion of the carbon blocks by 38.3% and 65.9%, respectively, and increased the thermal conductivity by 57.1%. The CB absorbed the low molecular weight compounds of binder pitch and induced the release of generated gases during the carbonization process to decrease porosity, and the thermal conductivity of the carbon block increased.
Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2
Authors: Shaath Hibah , Alajez Nehad M.
Published: September 2020

The continuous and rapid emergence of new viral strains calls for a better understanding of the fundamental changes occurring within the host cell upon viral infection. In this study, we analyzed RNA-seq transcriptome data from Calu-3 human lung epithelial cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) compared to five other viruses namely, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East Respiratory Syndrome (SARS-MERS), influenzavirus A (FLUA), influenzavirus B (FLUB), and rhinovirus (RHINO) compared to mock-infected cells and characterized their coding and noncoding RNA transcriptional portraits. The induction of interferon, inflammatory, and immune response was a hallmark of SARS-CoV-2 infection. Comprehensive bioinformatics revealed the activation of immune response and defense response to the virus as a common feature of viral infection. Interestingly however, the degree of functional categories and signaling pathways activation varied among different viruses. Ingenuity pathways analysis highlighted altered conical and casual pathways related to TNF, IL1A, and TLR7, which are seen more predominantly during SARS-CoV-2 infection. Nonetheless, the activation of chemotaxis and lipid synthesis was prominent in SARS-CoV-2-infected cells. Despite the commonality among all viruses, our data revealed the hyperactivation of chemotaxis and immune cell trafficking as well as the enhanced fatty acid synthesis as plausible mechanisms that could explain the inflammatory cytokine storms associated with severe cases of COVID-19 and the rapid spread of the virus, respectively.
Insights into the Complement System of Tunicates: C3a/C5aR of the Colonial Ascidian Botryllus schlosseri
Authors: Peronato Anna , Franchi Nicola , Ballarin Loriano
Published: September 2020

As an evolutionary ancient component of the metazoan immune defense toolkit, the complement system can modulate cells and humoral responses of both innate and (in jawed vertebrates) adaptive immunity. All the three known complement-activation pathways converge on the cleavage of C3 to C3a and C3b. The anaphylatoxin C3a behaves as a chemokine in inflammatory responses, whereas C3b exerts an opsonic role and, ultimately, can activate the lytic pathway. C3aR, one of the mammalian receptors for C3a, is a member of the G-protein-coupled receptor family sharing seven transmembrane alpha helixes. C3aR can act as a chemokine and recruit neutrophils, triggering degranulation and respiratory burst, which initiates an inflammatory reaction. Mining the transcriptome of the colonial ascidian Botryllus schlosseri, we identified a transcript showing homology with both mammalian C3aR and C5aR. The gene (bsc3/c5ar) is actively transcribed in morula cells, the circulating immunocyte triggering the inflammatory reactions in response to the recognition of nonself. Its transcription is modulated during the recurrent cycles of asexual reproduction known as blastogenetic cycles. Moreover, the treatment of hemocytes with C3aR agonist, induces a significant increase in the transcription of BsC3, revealing the presence of an autocrine feedback system able to modulate the expression of C3 in order to obtain a rapid clearance of potentially dangerous nonself cells or particles. The obtained results support the previously proposed role of complement as one of the main humoral components of the immune response in tunicates and stress the importance of morula cells in botryllid ascidian innate immunity.
Implication of Potassium Channels in the Pathophysiology of Pulmonary Arterial Hypertension
Authors: Le Ribeuz Hélène , Capuano Véronique , Girerd Barbara , Humbert Marc , Montani David , Antigny Fabrice
Published: September 2020

Pulmonary arterial hypertension (PAH) is a rare and severe cardiopulmonary disease without curative treatments. PAH is a multifactorial disease that involves genetic predisposition, epigenetic factors, and environmental factors (drugs, toxins, viruses, hypoxia, and inflammation), which contribute to the initiation or development of irreversible remodeling of the pulmonary vessels. The recent identification of loss-of-function mutations in KCNK3 (KCNK3 or TASK-1) and ABCC8 (SUR1), or gain-of-function mutations in ABCC9 (SUR2), as well as polymorphisms in KCNA5 (Kv1.5), which encode two potassium (K+) channels and two K+ channel regulatory subunits, has revived the interest of ion channels in PAH. This review focuses on KCNK3, SUR1, SUR2, and Kv1.5 channels in pulmonary vasculature and discusses their pathophysiological contribution to and therapeutic potential in PAH.
Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols
Authors: Chang Chia-Chen , Hsu Tsz-Lian , Chen Chie-Pein , Chen Chen-Yu
Published: September 2020

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.
Reductive Dechlorination of Chloroacetamides with NaBH4 Catalyzed by Zero Valent Iron, ZVI, Nanoparticles in ORMOSIL Matrices Prepared via the Sol-Gel Route
Authors: Meistelman Michael , Meyerstein Dan , Bardea Amos , Burg Ariela , Shamir Dror , Albo Yael
Published: September 2020

The efficient reductive dechlorination, as remediation of dichloroacetamide and monochloroacetamide, toxic and abundant pollutants, using sodium borohydride catalyzed by zero valent iron nanoparticles (ZVI-NPs), entrapped in organically modified hybrid silica matrices prepared via the sol-gel route, ZVI@ORMOSIL, is demonstrated. The results indicate that the extent of the dechlorination reaction depends on the nature of the substrate and on the reaction medium. By varying the amount of catalyst or reductant in the reaction it was possible to obtain conditions for full dechlorination of these pollutants to nontoxic acetamide and acetic acid. A plausible mechanism of the catalytic process is discussed. The present work expands the scope of ZVI-NP catalyzed reduction of polluting compounds, first reports the catalytic parameters of chloroacetamide reduction, and offers additional insight into the heterogeneous catalyst structure of M0@ORMOSIL sol-gel. The ZVI@ORMOSIL catalyst is ferromagnetic and hence can be recycled easily.
Hydrodechlorination of Different Chloroaromatic Compounds at Room Temperature and Ambient Pressure—Differences in Reactivity of Cu- and Ni-Based Al Alloys in an Alkaline Aqueous Solution
Authors: Weidlich Tomáš , Kamenická Barbora , Melánová Klára , Čičmancová Veronika , Komersová Alena , Čermák Jiří
Published: September 2020

It is well known that the hydrodechlorination (HDC) of chlorinated aromatic contaminants in aqueous effluents enables a significant increase in biodegradability. HDC consumes a low quantity of reactants producing corresponding non-chlorinated and much more biodegradable organic compounds. Two commonly used precious metals free Al alloys (Raney Al-Ni and Devarda’s Al-Cu-Zn) were compared in reductive action in an alkaline aqueous solution. Raney Al-Ni alloy was examined as a universal and extremely effective HDC agent in a diluted aqueous NaOH solution. The robustness of Raney Al-Ni activity is illustrated in the case of HDC of polychlorinated aromatic compounds mixture in actual waste water. In contrast, Devarda’s Al-Cu-Zn alloy was approved as much less active for HDC of the tested chlorinated aromatic compounds, but with a surprisingly high selectivity on cleavage of C-Cl bonds in the meta and sometimes the ortho position in chlorinated aniline and sometimes chlorinated phenol structures. The reaction of both tested alloys with chlorinated aromatic compounds in the aqueous NaOH solution is accompanied by dissolution of aluminum. Dissolved Al in the alkaline HDC reaction mixture is very useful for subsequent treatment of HDC products by coagulation and flocculation of Al(OH)3 caused by simple neutralization of the alkaline aqueous phase after the HDC reaction.
Feasibility Study of Plasma-Catalytic Ammonia Synthesis for Energy Storage Applications
Authors: Rouwenhorst Kevin H. R. , Lefferts Leon
Published: September 2020

Plasma catalysis has recently gained traction as an alternative to ammonia synthesis. The current research is mostly fundamental and little attention has been given to the technical and economic feasibility of plasma-catalytic ammonia synthesis. In this study, the feasibility of plasma-catalytic ammonia is assessed for small-scale ammonia synthesis. A brief summary of the state of the art of plasma catalysis is provided as well as a targets and potential avenues for improvement in the conversion to ammonia, ammonia separation and a higher energy efficiency. A best-case scenario is provided for plasma-catalytic ammonia synthesis and this is compared to the Haber-Bosch ammonia process operated with a synthesis loop. An ammonia outlet concentration of at least 1.0 mol. % is required to limit the recycle size and to allow for efficient product separation. From the analysis, it follows that plasma-catalytic ammonia synthesis cannot compete with the conventional process even in the best-case scenario. Plasma catalysis potentially has a fast response to intermittent renewable electricity, although low pressure absorbent-enhanced Haber-Bosch processes are also expected to have fast responses to load variations. Low-temperature thermochemical ammonia synthesis is expected to be a more feasible alternative to intermittent decentralized ammonia synthesis than plasma-catalytic ammonia synthesis due to its superior energy efficiency.
Actomyosin Contractility in the Generation and Plasticity of Axons and Dendritic Spines
Authors: Mikhaylova Marina , Rentsch Jakob , Ewers Helge
Published: September 2020

Actin and non-muscle myosins have long been known to play important roles in growth cone steering and neurite outgrowth. More recently, novel functions for non-muscle myosin have been described in axons and dendritic spines. Consequently, possible roles of actomyosin contraction in organizing and maintaining structural properties of dendritic spines, the size and location of axon initial segment and axonal diameter are emerging research topics. In this review, we aim to summarize recent findings involving myosin localization and function in these compartments and to discuss possible roles for actomyosin in their function and the signaling pathways that control them.
A Numerical Analysis of the Effects of Supercritical CO2 Injection on CO2 Storage Capacities of Geological Formations
Authors: Khudaida Kamal Jawher , Das Diganta Bhusan
Published: September 2020

One of the most promising means of reducing carbon content in the atmosphere, which is aimed at tackling the threats of global warming, is injecting carbon dioxide (CO2) into deep saline aquifers (DSAs). Keeping this in mind, this research aims to investigate the effects of various injection schemes/scenarios and aquifer characteristics with a particular view to enhance the current understanding of the key permanent sequestration mechanisms, namely, residual and solubility trapping of CO2. The paper also aims to study the influence of different injection scenarios and flow conditions on the CO2 storage capacity and efficiency of DSAs. Furthermore, a specific term of the permanent capacity and efficiency factor of CO2 immobilization in sedimentary formations is introduced to help facilitate the above analysis. Analyses for the effects of various injection schemes/scenarios and aquifer characteristics on enhancing the key permanent sequestration mechanisms is examined through a series of numerical simulations employed on 3D homogeneous and heterogeneous aquifers based on the geological settings for Sleipner Vest Field, which is located in the Norwegian part of the North Sea. The simulation results highlight the effects of heterogeneity, permeability isotropy, injection orientation and methodology, and domain-grid refinement on the capillary pressure–saturation relationships and the amounts of integrated CO2 throughout the timeline of the simulation via different trapping mechanisms (solubility, residual and structural) and accordingly affect the efficiency of CO2 sequestration. The results have shown that heterogeneity increases the residual trapping of CO2, while homogeneous formations promote more CO2 dissolution because fluid flows faster in homogeneous porous media, inducing more contact with fresh brine, leading to higher dissolution rates of CO2 compared to those in heterogeneous porous medium, which limits fluid seepage. Cyclic injection has been shown to have more influence on heterogenous domains as it increases the capillary pressure, which forces more CO2 into smaller-sized pores to be trapped and exposed to dissolution in the brine at later stages of storage. Storage efficiency increases proportionally with the vertical-to-horizontal permeability ratio of geological formations because higher ratios facilitate the further extent of the gas plume and increases the solubility trapping of the integrated gas. The developed methodology and the presented results are expected to play key roles in providing further insights for assessing the feasibility of various geological formations for CO2 storage.
Corrosion Inhibitory Effects of Mullite in Concrete Exposed to Sulfuric Acid Attack
Authors: Taheri Shima , Delgado Gerardo Pareja , Agbaje Oluwatoosin B. A. , Giri Paritosh , Clark Simon Martin
Published: September 2020

Prolonged exposure to low pH conditions affects the durability of concrete. In this work, the effect of mullite, aluminum silicate, on the strength and the acid corrosion of mortar and concrete under induced accelerated conditions in sulfuric acid solutions at pH of 0.25 and 1 was studied. The characterization of physicochemical changes was performed using techniques including compressive strength, scanning electron microscopy, micro-X-ray fluorescence spectrometry, and the Vickers hardness test. The results indicate that the addition of mullite does not have any significant effect on the overall strength of mortar and concrete samples, while it significantly increases their resistance to corrosion caused by sulfate attack by 90%, therefore, it is expected to increase the life span and decrease the maintenance costs of concrete pipes subjected to acid corrosion in sewer environments. The inhibition efficiency is observed to be sensitive to acid concentration and was improved with increase in the amount of mullite in samples.
Gloss, Light Reflection and Iridescence in Ceramic Tile Enamels Containing ZrO2 and ZnO
Authors: Echarri-Iribarren Víctor , Rizo-Maestre Carlos
Published: September 2020

Ceramic claddings on building facades not only present functional qualities and good resistance; they also add value to the architecture due to their qualities of light reflection, gloss and iridescence. The colour ranges produced by some enamel application techniques can vary widely. They change depending on one’s angle of vision and movement, colours in the surroundings, sunlight and their angle of incidence. In addition, the iridescent-pearl effect produced by light diffraction can lead to beautiful goniochromatic colours. This study analyses the production of square tiles of stoneware manufactured by extrusion, and their application to the Faculty of Education of the University of Alicante (FEUA) (Spain). Applying an enamel containing zirconium silicate ZrSiO4 and other metals such as Zn and Al produces iridescence-like effects. The physical-chemical properties of enamel and gloss values were characterised. A colorimetric characterisation was conducted by evaluating goniochromatic or iridescent colours, measuring the light’s spectral radiance factor, and comparing these results with other ceramic tiles of marked iridescent effects, with the presence of a final layer of anatase TiO2 enamel.
Spontaneous and Induced Animal Models for Cancer Research
Authors: Onaciu Anca , Munteanu Raluca , Munteanu Vlad Cristian , Gulei Diana , Raduly Lajos , Feder Richard-Ionut , Pirlog Radu , Atanasov Atanas G. , Korban Schuyler S. , Irimie Alexandru , Berindan-Neagoe Ioana
Published: September 2020

Considering the complexity of the current framework in oncology, the relevance of animal models in biomedical research is critical in light of the capacity to produce valuable data with clinical translation. The laboratory mouse is the most common animal model used in cancer research due to its high adaptation to different environments, genetic variability, and physiological similarities with humans. Beginning with spontaneous mutations arising in mice colonies that allow for pursuing studies of specific pathological conditions, this area of in vivo research has significantly evolved, now capable of generating humanized mice models encompassing the human immune system in biological correlation with human tumor xenografts. Moreover, the era of genetic engineering, especially of the hijacking CRISPR/Cas9 technique, offers powerful tools in designing and developing various mouse strains. Within this article, we will cover the principal mouse models used in oncology research, beginning with behavioral science of animals vs. humans, and continuing on with genetically engineered mice, microsurgical-induced cancer models, and avatar mouse models for personalized cancer therapy. Moreover, the area of spontaneous large animal models for cancer research will be briefly presented.
Mouthwash Effects on LGG-Integrated Experimental Oral Biofilms
Authors: Jiang Qingru , Kainulainen Veera , Stamatova Iva , Janket Sok-Ja , Meurman Jukka H. , Korpela Riitta
Published: September 2020

In order to investigate the effects of mouthwashes on oral biofilms with probiotics, we compared in biofilms the susceptibility to mouthwashes of probiotic Lactobacillus rhamnosus GG (LGG) and oral pathogens Streptococcus mutans, Streptococcus sanguinis, and Candida albicans. We also evaluated these pathogens’ susceptibility to the mouthwashes and their recovery after mouthwash-rinsing in biofilms with/without LGG. First, 1-day-/3-day-old LGG-integrated multi-species biofilms were exposed for 1 min to mouthwashes containing chlorhexidine, essential oils, or amine fluoride/stannous fluoride. Cells were plate-counted and relative survival rates (RSRs) of LGG and pathogens calculated. Second, 1-day-/3-day-old multispecies biofilms with and without LGG were exposed for 1 min to mouthwashes; cells were plate-counted and the pathogens’ RSRs were calculated. Third, 1-day-old biofilms were treated for 1 min with mouthwashes. Cells were plate-counted immediately and after 2-day cultivation. Recovery rates of pathogens were calculated and compared between biofilms with/without LGG. Live/Dead® staining served for structural analyses. Our results showed that RSRs of LGG were insignificantly smaller than those of pathogens in both 1-day and 3-day biofilms. No significant differences appeared in pathogens’ RSRs and recovery rates after treatment between biofilms with/without LGG. To conclude, biofilm LGG was susceptible to the mouthwashes; but biofilm LGG altered neither the mouthwash effects on oral pathogens nor affected their recovery.
Treatment Success and User-Friendliness of An Electric Toothbrush App: A Pilot Study
Authors: Humm Viviane , Wiedemeier Daniel , Attin Thomas , Schmidlin Patrick , Gartenmann Stefanie
Published: September 2020

Electronic and mobile health (eHealth/mHealth) are rapidly growing areas in medicine and digital technologies are gaining importance. In dentistry, digitalization is also an emerging topic, whereby more and more applications are being offered. As an example, using real-time feedback, digital application software (an app) was designed to help users brush their teeth more accurately. However, there is no data on the effectiveness and haptic of such apps. Therefore, a single-blinded, randomized controlled clinical trial was designed: twenty volunteers received an electric toothbrush with an associated app to assess whether the app-assisted toothbrushing is better than without. After a short period of familiarization with the electric toothbrush, plaque index (O‘Leary et al. 1972) was recorded and subjects were assigned to the test (with app; n = 10) or the control group (no app; n = 10). At the end of the 2-week pilot study period, plaque was again assessed and participants in the test group completed a questionnaire about the app’s user-friendliness. Statistical analysis revealed no significant differences between the test and control groups. The plaque index improved on average by 8.5% points in the test and 4.7% points in the control group. Fifty percent of the test group participants were of the opinion that they had achieved better cleaning results and would recommend the app to others, although the app contributed only marginally to increased plaque removal. However, such apps may nevertheless be helpful as motivational tools, especially when tracking and monitoring cleaning data. Therefore, more development and research on this topic is indicated.
Management of Peri-Implant Diseases: A Survey of Australian Periodontists
Authors: Khan Ahsen , Sharma Dileep
Published: September 2020

Background/Aim: This survey-based study aims to explore the clinical management protocols of followed by Australian periodontists in relation to peri-implant diseases. Materials and Methods: A five-part online questionnaire was developed and administered through email. Descriptive statistics were used for analysis, with the univariate associations between a categorical outcome and the variables evaluated using Pearson’s Chi-squared test. Results: The survey yielded 99 responses, resulting in a response rate of 41.8%. Most participants were male and aged 35–44 years. More than a quarter of practitioners had been placing implants for 6–10 years and almost two-fifths of practitioners placed 1–10 implants per month. The estimated prevalence of peri-implant mucositis and peri-implantitis in the general Australian population was 47% and 21%, respectively. Practitioners reported using systemic antibiotics to manage peri-implant mucositis (7%) and (72%) peri-implantitis lesions, with a combination of amoxicillin and metronidazole. Most common treatment modalities were oral hygiene instructions, nonsurgical debridement and antimicrobial gel/rinse. Surgical debridement and systemic antibiotics were also often used for peri-implantitis treatment. Practitioners preferred a 3-month clinical follow-up and 6-month radiographic evaluation. Furthermore, three-quarters of practitioners rated their management as moderately effective, although upwards of nine-tenths expressed the need for further training and awareness. Conclusion: This study confirms a significant use of empirical treatment modalities due to lack of standard therapeutic protocol. However, some approaches followed by the specialists may provide a basis to formulate a therapeutic protocol for peri-implant disease management.
Formative and Pilot Study for an Effectiveness-Implementation Hybrid Cluster Randomized Trial to Incorporate Oral Health Activities into Pediatric Well-Child Visits
Authors: Nelson Suchitra , Slusar Mary Beth , Curtan Shelley , Selvaraj David , Hertz Andrew
Published: September 2020

Background: Dental caries in pediatric patients are noted to have broad impacts on systemic health and well-being. Thus, utilizing an effectiveness-implementation hybrid I design, the Pediatric Providers Against Cavities in Children’s Teeth (PACT) trial is investigating multi-level interventions at the practice (incorporation of oral health in electronic medical record [EMR]) and provider levels (theory-based didactic and skills training to communicate oral health facts to parent/caregiver, give a prescription to see a dentist and a list of area dentists) to increase dental utilization among 3 to 6 year old Medicaid-enrolled children attending well-child visits (WCV). The formative and pilot work for the larger main trial are presented. Methods: Formative work—Focus groups with 26 participants (Community leaders, providers, parent/caregivers); and key informant interviews with practice leadership (n = 4). Topics discussed were: core oral health (OH) information to communicate at WCVs and study logistics. Transcripts were coded and analyzed using Atlas.ti; Pilot study was refined using the formative findings and was conducted at two pediatric practices to test the implementation of: the provider didactic and skills training curriculum; EMR incorporation of four OH questions; logistics of incorporating OH activities at a WCV; and parent/caregiver recruitment. Results: Formative work showed that providers and parent/caregivers required knowledge of dental caries, and a list of area Medicaid-accepting dentists. Providers and practice leadership advised on the logistics of incorporating oral health into WCVs. All groups suggested asking parent/caregivers their preferred method of contact and emphasizing importance of OH to motivate participation. Utilizing these findings, the curriculum and protocol was revised. The pilot study in two practices successfully implemented the protocol as follows: all seven providers were trained in two 45 min didactic education and skills session; incorporation of OH questions into practices EMR; recruited 86 child-parent dyads (95% participation) at the WCV; providers delivered the OH intervention to parent/caregivers in <2 min and 90% completed EMR documentation of OH questions. These findings were instrumental in finalizing the main PACT trial in 18 practices. The RE-AIM framework is used in the main trial to collect effectiveness and implementation measures at baseline and follow-up visits. Conclusions: The formative and pilot findings were instrumental in refining the OH intervention and protocol which has resulted in successful implementation of the main trial. Trial Registration: Clinical trials.gov, Registered 9 November 2017, NCT03385629.
Developing a Messaging Graphic for Storage Times of Refrigerated Ready to Eat (RTE) Foods for a Consumer Food Safety Health Campaign
Authors: Chambers Delores , Chambers Edgar , Godwin Sandria , Doan Alisa , Cates Sheryl
Published: September 2020

This study developed and evaluated risk communication messages for ready to eat (RTE) foods targeted towards consumer storage practices in a food safety health campaign. Concepts were determined from a fractional factorial design of five categories of attributes potentially present in health promotion: title, message, graphic, slogan, and icon. Consumers viewed a subset of concepts and scored how useful the concept was in remembering to throw away RTE foods that were stored too long. Regression analysis determined which combinations of message attributes were most likely to result in using the information to throw out foods, which could help prevent foodborne illness. Findings showed that for this type of information, a graphic is a critical element for the printed schematic. The slogan (i.e., a short statement similar to a jingle or tag-line in a commercial) may be important to consumers, but the icon was not important.
Oxygen-Plasma Surface Treatment of an Electrode Sheet Using Carbon from Japanese Distilled Liquor Waste for Double-layer Capacitors
Authors: Tashima Daisuke , Hirano Masaki , Kitazaki Satoshi , Eguchi Takuya , Kumagai Seiji
Published: September 2020

Oxygen-plasma treatment was performed on an activated carbon sheet obtained from shochu (Japanese distilled liquor) waste using a high-frequency plasma generator. The capacitances of the activated carbon sheet electrode subjected to surface treatment were measured using cyclic voltammetry. The best results were obtained with a processing time of 60 min, an output power of 40 W, an interelectrode distance of 13 cm and an oxygen pressure of 40 Pa. The maximum capacitance was 247 F g−1. Investigation of the surface functional groups of the activated carbon sheet revealed that the oxygen-containing functional groups modified on the surface of the activated carbon sheet electrode contributed to the improvement in the capacitance. High-performance electric double-layer capacitors can be realized using the developed electrodes.
Hybrid Non-Volatile Flip-Flops Using Spin-Orbit-Torque (SOT) Magnetic Tunnel Junction Devices for High Integration and Low Energy Power-Gating Applications
Authors: Park Jaeyoung
Published: September 2020

This paper presents two novel hybrid non-volatile flip-flops (NVFFs) comprised of the conventional CMOS flip-flop for static storage in normal operations and Spin-Orbit-Torque Magnetic Tunnel Junction (SOT-MTJ) devices for temporary storage during power gating. The proposed NVFFs re-utilize a part of the standard CMOS flip-flop infrastructure for storing and restoring data onto MTJs for reducing the area. Furthermore, the proposed NVFFs re-use a write current, which is used for storing an MTJ, to write the other MTJ at a time, resulting in 50% storing energy reduction. To reduce the area further, the number of external terminals of an MTJ is reduced by shorting the shorting physical terminals. Removing a terminal using the proposed STT-Like SOT configuration results in fewer transistors to control. The proposed NVFF circuits are evaluated using a compact MTJ model targeting implementation in a 14-nm technology node. Analysis indicates that area overheads are only 10.3% and 6.9% compared to the conventional D flip-flop because three or two minimum-sized NMOS transistors are added for accessing MTJs. Compared to the best previously known NVFFs, the proposed NVFF has an improvement by a factor of 2–8 in terms of the area overhead.
Bottleneck Based Gridlock Prediction in an Urban Road Network Using Long Short-Term Memory
Authors: Mon Ei Ei , Ochiai Hideya , Saivichit Chaiyachet , Aswakul Chaodit
Published: September 2020

The traffic bottlenecks in urban road networks are more challenging to investigate and discover than in freeways or simple arterial networks. A bottleneck indicates the congestion evolution and queue formation, which consequently disturb travel delay and degrade the urban traffic environment and safety. For urban road networks, sensors are needed to cover a wide range of areas, especially for bottleneck and gridlock analysis, requiring high installation and maintenance costs. The emerging widespread availability of GPS vehicles significantly helps to overcome the geographic coverage and spacing limitations of traditional fixed-location detector data. Therefore, this study investigated GPS vehicles that have passed through the links in the simulated gridlock-looped intersection area. The sample size estimation is fundamental to any traffic engineering analysis. Therefore, this study tried a different number of sample sizes to analyze the severe congestion state of gridlock. Traffic condition prediction is one of the primary components of intelligent transportation systems. In this study, the Long Short-Term Memory (LSTM) neural network was applied to predict gridlock based on bottleneck states of intersections in the simulated urban road network. This study chose to work on the Chula-Sathorn SUMO Simulator (Chula-SSS) dataset. It was calibrated with the past actual traffic data collection by using the Simulation of Urban MObility (SUMO) software. The experiments show that LSTM provides satisfactory results for gridlock prediction with temporal dependencies. The reported prediction error is based on long-range time dependencies on the respective sample sizes using the calibrated Chula-SSS dataset. On the other hand, the low sampling rate of GPS trajectories gives high RMSE and MAE error, but with reduced computation time. Analyzing the percentage of simulated GPS data with different random seed numbers suggests the possibility of gridlock identification and reports satisfying prediction errors.
Neuromorphic Computing Using Emerging Synaptic Devices: A Retrospective Summary and an Outlook
Authors: Park Jaeyoung
Published: September 2020

In this paper, emerging memory devices are investigated for a promising synaptic device of neuromorphic computing. Because the neuromorphic computing hardware requires high memory density, fast speed, and low power as well as a unique characteristic that simulates the function of learning by imitating the process of the human brain, memristor devices are considered as a promising candidate because of their desirable characteristic. Among them, Phase-change RAM (PRAM) Resistive RAM (ReRAM), Magnetic RAM (MRAM), and Atomic Switch Network (ASN) are selected to review. Even if the memristor devices show such characteristics, the inherent error by their physical properties needs to be resolved. This paper suggests adopting an approximate computing approach to deal with the error without degrading the advantages of emerging memory devices.