bluuebunny/crossref_metadata_2025 huggingface dataset:
The moderating role of audit quality between earning management and sustainable investment opportunities: Evidences from gulf cooperation council countries
Authors: Alsmady Ahnaf Ali
Published: March 2023

This study examines the relationship between the earning management and audit quality on sustainable investment opportunities in the Gulf Cooperation Council (GCC) countries, including, Bahrain, Oman, Qatar, Saudi Arabia, Kuwait and United Arab Emirates. Further, moderating relationship of audit quality between earnings management and sustainable investment is also investigated in the present study. This study includes the data of 1337 companies and their-year observations from the period of 2011 to 2017. This study used the Panel Data Ordinary Least Squares with correlated random effect to examine the direct relationship and the moderated multiple regression to examine the moderating effects of audit quality on the earnings management and sustainable investment opportunities relationship. The results demonstrate that the earnings’ management encompasses a significant negative relationship with sustainable investment opportunities but on the other side, it also displays a positive relationship between audit quality and sustainable investment opportunities. Furthermore, the study investigates the moderating effect of audit quality on the relationship of earning management and sustainable in-vestment opportunities. Empirical evidence suggests that the audit quality plays a vital role in mitigating the agency theory problem and reducing the information asymmetry. These findings suggest that the big audit firms act as a good governance representatives for the management and it further enhances the credibility of accounting information for the new investors. This research has several usages for policymakers and investors in the GCC. The investors should assess their choice when they are investing in firms. The big auditing firms mitigates earnings management, which may raise sustainable investment opportunities. Thus, listed firms in the GCC should implement governance and disclosure practices that strengthen the credibility of financial earnings and reduce the danger for investors who later discovers poor investment decisions.
Inflation, the global financial crisis, and COVID-19 pandemic
Authors: Jałtuszyk Grzegorz
Published: March 2023

In the years 2021–2022, world inflation has drastically increased. The analyses of the main characteristics of the 2007–2009 financial crisis and of the economic crisis caused by the COVID-19 pandemic as well as of the rise in total debt of the non-financial sector suggest that the growth in inflation was triggered by excessive debt growth of the government sector in 2020–2021. In that period, the main goal of the debt growth of the government sector was to finance the aid schemes limiting the negative effects of the COVID-19 pandemic. These aid programmes were indispensable to avoid deep and long-term global economic recession, however, their value was too high to keep inflation in the world under control. The fiscal intervention of particular countries should be co-ordinated internationally, as the cumulative result of the operations undertaken by particular states might have detrimental effects globally.
Pax transactivation domain-interacting protein is required for preserving hematopoietic stem cell quiescence via regulating lysosomal activity
Authors: Zhang Tong , Cui Manman , Li Yashu , Cheng Ying , Gao Zhuying , Wang Jing , Zhang Tiantian , Han Guoqiang , Yin Rong , Wang Peipei , Tian Wen , Liu Weidong , Hu Jin , Wang Yuhua , Liu Zheming , Zhang Haojian
Published: March 2023

Hematopoietic stem cells (HSCs) maintain lifetime whole blood hematopoiesis through self-renewal and differentiation. To sustain HSC stemness, most HSCs reside in a quiescence state, which is affected by diverse cellular stress and intracellular signal transduction. How HSCs accommodate those challenges to preserve lifetime capacity remains elusive. Here we show that Pax transactivation domain-interacting protein (PTIP) is required for preserving hematopoietic stem cell quiescence via regulating lysosomal activity. Using a genetic knockout mouse model to specifically delete Ptip in HSCs, we find that loss of Ptip promotes HSCs exiting quiescence, and results in functional exhaustion of HSCs. Mechanistically, Ptip loss increases lysosomal degradative activity of HSCs. Restraining lysosomal activity restores the quiescence and repopulation potency of Ptip-/- HSCs. Additionally, PTIP interacts with SMAD2/3 and mediates TGFβ signaling-induced HSC quiescence. Overall, our work uncovers a key role of PTIP in sustaining HSC quiescence via regulating lysosomal activity.
Prescripción de las acciones monitorias y los procesos ordinarios para el cobro de las obligaciones
Authors: Ramos Núñez Gissela Estefanía , Arroba López David Alejandro
Published: March 2023

Introducción: La temática a investigar se enmarca en las necesidades de saber el vacío legal que existe del tiempo de prescripción para cobrar la obligación por procedimiento monitorio, Estos procedimientos otorga al actor la facultad de ejercer la acción de cobro por una deuda dineraria, líquida, exigible y vencida, como consta en el artículo 356 del COGEP toda vez que no exceda de cincuenta Salarios Básicos Unificados. Objetivo: El objetivo de la presente investigación ha sido analizar el cobro de obligaciones a través de procedimiento monitorio a fin de establecer la necesidad de subsanar el vacío legal que existe respecto del tiempo de prescripción de estas. Metodología: La investigación se enmarca en un método sistemático, así como el analítico - sintético y deductivo – inductivo, el enfoque empleado es el cualitativo. Así también se ha elaborado un trabajo académico de tipo documental histórico descriptivo y de revisión. Resultados: Se analizó la prueba que acredita una relación previa entre acreedor y deudor, cuando el documento -que se pretende cobrar- haya sido creado unilateralmente por el acreedor. Conclusiones: Sobre la acción de cobro, el COGEP no estatuye tiempo alguno para la prescripción generando así un vacío estructural que resulta ineludible subsanar. Área de estudio general: Jurisprudencia y Ciencias Sociales. Área de estudio específica: Derecho civil.
PISA Assessment on Reading Literacy Competency : Evidence from Students in Urban, Mountainous and Island Areas
Authors: Amir Johar , Dalle Ambo , Dj Sulastriningsih , Irmawati Irmawati
Published: March 2023

This research aims to analyze the reading literacy competence of students in class VIII junior high school in Pangkajene and Islands Regency to find, understand, and evaluate information from reading texts. The research used the descriptive quantitative method. The population in this study were all class VIII students spread throughout Pangkajene and Islands Regency were divided into urban, mountainous and island areas totaling 114 schools where the sample selection used purposive sampling and cluster sampling techniques. The technique for collecting data is the test technique. The research instrument was PISA’s questions in 2021. The test PISA’s results showed that 26.7- 60% of students in Class VIII from the three schools in Pangkajene and Islands Regencies had very low competence in finding information, with a score of <54. Then in understanding the reading text, they could only obtain an average score of 33.03. As for the ability to evaluate text, 96.6% of students were in the low category, and only 3.4% were in the high category. This research indicated that students at the junior high school level needed the PISA assessment system to improve the quality of educational competence in Indonesia towards international education standards in the 21st century, especially in reading literacy. The PISA assessment focuses on improving the quality of teaching by teachers and learning in individual students. So that regular PISA assessments can help all students to achieve high competence if the government, school principals and teachers work together to implement and evaluate the PISA assessment on the reading literacy method.
Characteristics of Unexposed Proliferative Diabetic Retinopathy: An Optical Coherence Tomography Angiography Study
Authors: Kim Hyunyeon , Lee Donghun , Yoon Sook Hyun , Lee Geun Woo
Published: March 2023

Purpose: To compare optical coherence tomography angiography (OCTA) findings between severe non-proliferative diabetic retinopathy (NPDR) and unexposed proliferative diabetic retinopathy (PDR), and identify predictive factors.Methods: Patients newly diagnosed with severe NPDR or unexposed PDR between January 2018 and December 2021 were reviewed retrospectively. Unexposed PDR was diagnosed using fluorescein fundus angiography, because new vessels could not be observed in the poster pole or clearly distinguished in the retinal periphery on wide fundus photography. Clinical features at the time of diagnosis, and OCTA measurements (mean vascular density, superficial capillary plexus (SCP) foveal avascular zone (FAZ) area, and mean retinal thickness), were compared between the two groups. Factors that could predict unexposed PDR were investigated using multivariate analysis with a generalized estimating equation.Results: A total of 61 severe NPDR and 23 unexposed PDR eyes were included. The unexposed PDR had significantly larger SCP-FAZ areas (p = 0.031) and lower total and parafoveal mean inner retinal thicknesses (p = 0.014 and p < 0.001, respectively). However, there were no differences in mean vascular density between the groups (p > 0.05). Multivariate analysis showed that SCP-FAZ area and parafoveal mean inner retinal thickness were significant predictors of unexposed PDR (p = 0.027 and p = 0.001, respectively).Conclusions: In severe NPDR patients, unexposed PDR may be considered a differential diagnosis when the SCP-FAZ area is large or the parafoveal mean inner retinal thickness is small.
Müsnedün İleyh ve Müsned Bağlamında Lafzî Mutâbakat ve Udûl
Authors: AKBAŞ Yaşar Fatih
Published: March 2023

Lafızlar ister umde görevi üstlensinler ister kayd/Fuzla (tekmile) olarak kullanılsınlar Arapça bir cümlede yanlış anlama ve karışıklığı engellemek gayesiyle cümle öğelerinin bir kısmının bir kısım öğelerle cinsiyet (müzekkerlik-müenneslik), belirlilik (marifelik-nekrelik), sayı ve i‘rab bakımından bazen tam bazen kısmî bir uyumu bulunmaktadır. Bu uyum okuyucu veya dinleyicinin hataya düşmesini engellemek, kelamı daha rahat anlamasını sağlamak açısından son derece önemlidir. Ne var ki bu uyum bazen kelimenin kendisinden kaynaklanan bir sebeple bazen de kullanımın değişmesine bağlı olarak ortadan kalkabilmektedir. Bu bir eksiklik olmayıp Arapçanın geniş bir kullanım alanı olmasından kaynaklanmaktadır. Bu çalışma bir karîne türü olan lafzî mutâbakatı, müsnedün ileyh (özne) ve müsned (yüklem) olarak isimlendirilen cümlenin temel unsurlarının birbirleriyle olan uyum ilişkisini, bu uyum ilişkisinin hangi durumlarda gerçekleştiğini ve udûl kavramı ile ifade edilen mutâbakatın hangi durumlarda ortadan kalktığı konusunu ele almayı hedeflemektedir. Zikri geçen hususlar dil bilgisi ve belâgat ilmi açısından ele alınarak bir sonuca varılmıştır.
Contribuições da Análise de Causa Raiz (RCA) na gestão de eventos adversos em Emergência – uma revisão de literatura
Authors: Souza Flávia Silva de , Calado Robisom , Reis Milena Estanislau Diniz Mansur dos , Santos Teresa de Lourdes da Rosa dos , Andrade Salua Ilza de , Doria Kelly Cristina Freire
Published: March 2023

O presente estudo busca identificar, na literatura, publicações que indiquem o uso da RCA como metodologia para a gestão de eventos na Emergência e suas contribuições para a equipe assistencial por meio de uma Revisão Sistemática de Literatura. A coleta de dados foi realizada através de busca virtual de artigos e estudos indexados nas Bases de Dados da Biblioteca Virtual da Saúde (BVS), Cochrane, Elsevier e Banco de dados da Biblioteca Digital Brasileira de Teses e Dissertações (BDTD). Como resultado, foram encontrados 17 estudos, que foram tratados por meio da Análise de Conteúdo, sendo distribuídos em três categorias, a saber: Uso da RCA em Serviço de Emergência, Recomendações e Limitações do uso da RCA – uma análise crítica e Uso de outras ferramentas para a Gestão de Eventos Adversos em Emergência. Conclui-se que, apesar de não ser utilizada em sua completude em alguns cenários de Emergência, a RCA, ainda é a ferramenta de escolha para a análise de causa raiz de eventos adversos. O presente estuda aponta a possibilidade de associação da metodologia DMAIC ao uso da ferramenta com vistas a reduzir tempo de respostas da análise, implantação de melhorias e controle dos resultados.
Identificação automatizada de textos em imagens com Amazon Rekognition
Authors: Barata Jardel Silas da Silva , Teixeira Lucas Ravele de Sousa , Silva Bruno Campos da , Conte Thiago Nicolau Magalhães de Souza , Santos Wilker José Caminha dos
Published: March 2023

O reconhecimento de texto em imagens é um desafio na área de visão computacional devido à variedade de fontes, qualidade da imagem, tamanho e cores presentes nas imagens. Nesse contexto, este trabalho tem como objetivo desenvolver uma aplicação de reconhecimento de textos em imagens utilizando a API Amazon Rekognition e avaliar sua precisão. Para isso, é proposto um algoritmo baseado em técnicas de deep learning capaz de alcançar uma precisão acima de 90% na localização e extração de texto nas imagens, utilizando métodos de extração de dados da função de detecção de texto da API Amazon Rekognition. Este artigo também tem o potencial de contribuir para o avanço de trabalhos futuros no campo da visão computacional, com ênfase na detecção de texto em imagens. Por fim, o estudo conclui que a API de detecção de texto da Amazon Rekognition é relevante na análise de dados, considerando que é treinada com grandes quantidades de dados de imagem para aprender características relevantes, alcançando uma precisão acima de 90%. No entanto, é necessário considerar que a qualidade da imagem e o tipo de fonte utilizada podem influenciar na precisão dos resultados.
Agronomic performance and morphogenic and structural characteristics of Axonopus aureus pastures under phosphate fertilization levels in the Roraima’s savannas
Authors: Costa Newton de Lucena , Magalhães João Avelar , Rodrigues Braz Henrique Nunes , Santos Francisco José de Seixas
Published: March 2023

The effect of phosphate fertilization (0, 30, 60 and 120 kg of P2O5 ha-1) on forage production and morphogenetic and structural characteristics of Axonopus aureus was evaluated under natural field conditions in the savannas of Roraima. Phosphate fertilization positively and significantly (P<0.05) affected green dry matter (GDM) production, absolute growth rate (AGR), number of tillers plant-1, number of leaves tiller-1 (NLT), average leaf size (ALS), leaf tiller area (LTA), appearance rates (LAR), expansion (LER) and leaf senescence rates. The maximum yields of GDM, AGR, LAR, LER, NLT, LTA and ALS were obtained with the application of 110.4; 108.5; 54.1; 83.5; 96.6; 59.8 and 90.6 kg of P2O5 ha-1, respectively. The efficiency of P utilization was inversely proportional to the P doses applied.
A ludicidade no ensino remoto: práxis docente ao processo de alfabetização de crianças
Authors: Lins Léia Soares , Vasconcelos Corina Fátima Costa , Justi Jadson
Published: March 2023

Este estudo teve como objetivo investigar a contribuição da ludicidade como estratégia metodológica na alfabetização de crianças do 1º ano do Ensino Fundamental no ensino remoto. Metodologicamente, a presente pesquisa se enquadra como qualitativa. A produção (coleta) de dados ocorreu por meio de observação direta e conversas informais que possibilitaram resultados com maior fidedignidade. As participantes da pesquisa foram duas professoras alfabetizadoras que ministram aulas no 1º ano do Ensino Fundamental. Os resultados obtidos demonstram que a ludicidade no ensino remoto, sob a perspectiva de alfabetização, está diretamente relacionada ao brincar, assim como no ensino presencial. Parte-se do pressuposto de que se houver ambiente, interações e situações pedagogicamente adequadas, as crianças usarão a linguagem da leitura e escrita em situações lúdicas favorecedoras ao processo de aprendizagem. Desta forma, conclui-se que há necessidade de oferecer meios favoráveis ao desenvolvimento de aulas remotas de forma que valorize a condição de ludicidade, uma vez que o novo formato educacional remoto se apresenta como algo que irá permanecer como avanço educacional da sociedade moderna.
Impacts of a conversation wheel between pregnant women and medicine students
Authors: Lins Anete Nailane Silva , Feitosa Joyci Kelly Estalião , Ribeiro Diego Ulisses de Melo , Lima Adna Cristina Marinho de , Siqueira Maíra Ribeiro de Santana , Vasconcelos Camila Soares de
Published: March 2023

Faced with a reality in which reliable popular knowledge and untruths routinely mix, it is necessary to investigate the impacts of dialogue on the formation of a critical conscience. In this sense, the experience report had the following objective: to report the experience of pregnant women and medical students in a conversation circle as a space for the collective construction of knowledge. Starting from an observational, descriptive study with a qualitative approach, it was observed, during the experience of the conversation wheel, the persistence of beliefs involving normal childbirth, given that, when listing truths and myths about humanized childbirth, many of the myths were part of the context of the pregnant women, including information still propagated by health professionals. It also became evident that the users were unaware of their rights during childbirth and how the absence of this information favors the occurrence of episodes of obstetric violence in a unique moment of significant vulnerability, childbirth. In addition, medical students involved in the event benefited from experiencing practices that go beyond theory, such as improving communication skills, autonomy in studies and transforming reality through knowledge. From the idealized dynamics based on PBL teaching, it is concluded that information sharing is the master way in demystifying inadequate knowledge and in building women aware of their rights, since female empowerment and knowledge are closely intertwined
Skrining senyawa Combretum Indicum sebagai inhibitor Caspase 3 secara In Silico
Authors:   Universitas Lambung Mangkurat Indonesia, Hadi Samsul , Salma Salma ,   Universitas Lambung Mangkurat Indonesia, khairunnisa Amalia ,   Universitas Lambung Mangkurat Indonesia, Kamelia Suci ,   Universitas Lambung Mangkurat Indonesia
Published: March 2023

Osteoarthritis ialah gangguan sendi degeneratif yang ditandai dengan erosi cartilage artikular. Salah satu protein yang mempunyai peran besar dalam penyakit ini adalah caspase sub type 3. Sehingga tujuan dari penelitian ini adalah skrining senyawa belanda yang berpotensi sebagai inhibitor caspase 3. Metode dari penelitian ini menggunakan seanyawa yang terkandung dalam Combretum indicum, data ini kemudian dilakukan docking menggunakan software PLANTS. Hasil dari penelitian ini adalah scor docking dari masing masing senyawa yaitu Isatin Sulfonamide -90,8975; Casuariin -92,4942; asquisqualic acid -90,8911; 1-desgalloyleugeniin -100,988; arachidonic acid -63,4613; Punicalin -88,2142; linoleic acid - 97,4111; gallic acid -59,1281; Rutin-95,3717; ellagic acid -63,6793; methyl ursolate -77,5611; flavogallonic acid -80,8221; Quisqualic acid -63,1281; brevifolin carboxylic acid -71,5203; Trigonelline-59,8987; Quercetin -74,4091; quinoline-4-carbonitrile -61,5971; β-sitosterol -87,3881; Pedunculagin-86,843 Lupeol -76,4221; Punicalagin -82,1754; linalool -67,8673; Eugeniin -100,7541.Oleh karena itu senyawa yang berpotensi sebagai inhibitor caspase 3 adalah Rutin, linoleic acid, asquisqualic acid, Eugenin, Casuariin, 1-desgalloyleugeniin.
Občianstvo Európskej únie
Authors:   Univerzita Pavla Jozefa Šafárika v Košiciach, Eštok Gabriel
Published: March 2023

Vysokoškolská učebnica Občianstvo Európskej únie je vnútorne rozdelené do štyroch častí. Prvá kapitola je zameraná na charakteristiku občianstva v historickom kontexte (koncepcie občianstva v staroveku, stredoveku a v novoveku). Druhá kapitola sa zaoberá teoretickým predstavením občianstva. Prichádza s charakteristikou občianstva, predstavuje spôsoby nadobúdania a straty občianstva a premenami občianstva v kontexte globalizácie a multikulturalizmu. Tretia kapitola sa zameriava samotným občianstvom Európskej únie, jeho historickým vývojom a procesom zavedenia a posilňovania občianstva v Európskej únii. Štvrtá kapitola predstavuje konkrétne práva občanov vyplývajúce z občianstva Európskej únie – právo na voľný pohyb, právo voliť a byť volený vo voľbách do orgánov samosprávnych obcí a voľby do Európskeho parlamentu, právo na diplomatickú a konzulárnu ochranu na území tretej krajiny, právo obrátiť sa petíciou na inštitúcie Európskej únie, právo na prístup k dokumentom inštitúcií a orgánov Únie a právo na iniciatívu občanov Európskej únie.
The Epidemic of Twins: The Challenge in Obstetrics and Gynaecology
Authors: González-Mesa Ernesto , Cazorla Olga , González-Valenzuela María José
Published: March 2023

In the past 50 years, the incidence of multiple pregnancy has increased dramatically due almost exclusively to two factors: delayed childbearing and assisted reproduction techniques (ARTs). Although the clinical guidelines and protocols currently endorsed by scientific societies have systematised the obstetric care required in multiple pregnancy, the effects of obstetric care beyond the perinatal period have seldom been evaluated. Twin deliveries also involve additional difficulties derived from the need for ‘simultaneous’ care of two fetuses during the expulsive period, during which the second twin is particularly vulnerable due to potential complications. Chorionicity, gestational age at birth, ART, birth order, and some parental variables, such as maternal age, have been studied as possible sources of neurodevelopmental delay in twins.
IRRIGATED MEADOW FARMING AND LYSIMETRIC STUDIES AT THE FEDERAL WILLIAMS RESEARCH CENTER OF FORAGE PRODUCTION AND AGROECOLOGY (To the 85th anniversary of B.I. Korotkov)
Authors: Grechishnikov Nikolay , Rodionov Vladimir , Trofimov Ilya
Published: March 2023

Agricultural land reclamation is of great importance in the creation and life of grass ecosystems. Even V.R. Williams in his book "Meadow Farming" (1901) wrote about the need for reclamation of meadows by drainage and irrigation, which is a necessary condition for obtaining high and stable yields. In 1969, an irrigation system on an area of 85 hectares was put into operation at the V.R. Williams Forage Research Institute, and in 1970 — a drainage system (closed drainage) on an area of 25 hectares. After the organization of the problem laboratory of "Irrigated Meadow Farming" in 1970, senior researcher and later the head of the laboratory B.I. Korotkov conducted research here on the study of irrigation regimes of hayfields and pastures. He was born on February 20, 1938 in the village of Mordviny, Kovrovsky district, Vladimir region. After graduating from school, he studied in 1955–1960 at the Timiryazev Agricultural Academy (TAA) with a degree in "Scientist agronomist" and worked for 5 years as an agronomist-meadow farmer of the Vladimir Meliovodstroy Trust. In 1965–1968, B.I. Korotkov entered and successfully graduated from the graduate school of the TAA, defended his PhD thesis on the topic "The experience of using irrigated by sprinkling cultural pastures in the Non-Chernozem zone". Together with his teacher, the famous scientist N.G. Andreev, B.I. Korotkov developed the topic of irrigation of pastures and hayfields of the Non-Chernozem region, including in combination with other methods of intensification of forage lands, developed criteria for pre-irrigation soil moisture, determined the capacity of the active water exchange layer, irrigation and irrigation norms. The result of their joint scientific activity was the book "Irrigated and cultural pastures" (1979), which summarizes the experience and achievements of science in our country and abroad. B.I. Korotkov made a significant contribution to the development of science and practice of irrigated meadow farming. He is the author of more than 100 scientific papers.
BIOSPHERE, NOOSPHERE AND AGRICULTURE (to the 160th anniversary of the birth V.I. Vernadsky)
Authors: Trofimov Ilya , Trofimova Lyudmila , Yakovleva Elena
Published: March 2023

The article is devoted to the outstanding Russian scientist V.I. Vernadsky, who is one of the founders of ecology. As a student of V.V. Dokuchaev, he developed his ideas of a systematic dynamic approach to the study of nature and introduced deep scientific ecological content into the concepts of the biosphere and the noosphere. V.I. Vernadsky is the founder of the complex of modern Earth sciences (geochemistry, biogeochemistry, radiology, hydrogeology) and the creator of many scientific schools. In his scientific work, he covered many scientific areas, from geology to the study of the role of living matter in geochemical cycles, from soil science to the biosphere, the increasing influence of scientific thought, human activity in the biosphere and its transformation into the noosphere. The importance of agriculture in the biosphere and noosphere is considered. Biosphere is an area of active life of the Earth (troposphere, hydrosphere and part of the lithosphere), the composition, structure and energy of which are mainly due to the activity of living organisms. The noosphere is its thinking shell. Agriculture, the most important part of which are grassland ecosystems, is an important component of the biosphere, a reproducible, autotrophic sustainable resource (energy, environmental, food and feed). In light of the pressing environmental problems facing the world, environmental education and environmental thinking are a priority for the development of the biosphere, the noosphere and agriculture.
Co-sensitization between legumes is frequently seen, but variable and not always clinically relevant
Authors: Smits Mark , Verhoeckx Kitty , Knulst André , Welsing Paco , de Jong Aard , Gaspari Marco , Ehlers Anna , Verhoeff Paulien , Houben Geert , Le Thuy-My
Published: March 2023

Background

Food allergy to peanut and soybean, both legumes, is highly prevalent. The consumption of other legumes and legume protein isolates, some of which may be considered novel foods, is increasing. This may lead to an increase in sensitization and allergy and may pose a risk for legume-allergic (e.g. peanut and soybean) patients due to cross-reactivity.

Objective

This study investigated the frequency of co-sensitization and co-allergy between legumes and the role of different protein families.

Methods

Six legume-allergic patient groups were included: peanut (n = 30), soybean (n = 30), lupine (n = 30), green pea (n = 30), lentil (n = 17), bean (n = 9). IgE binding to total extracts, protein fractions (7S/11S globulin, 2S albumin, albumin), and 16 individual proteins from 10 legumes (black lentil, blue lupine, chickpea, faba bean, green lentil, pea, peanut, soybean, white bean, and white lupine) was measured by line blot

Results

Co-sensitization varied from 36.7% to 100%. Mono-sensitization was only found in soybean (16.7%), peanut (10%), and green pea-allergic (3.3%) patients. A high frequency of co-sensitization between the 7S/11S globulin fractions of all 10 legumes and individual 7S and 11S globulins was observed. In peanut and soybean-allergic patients, co-allergies for other legumes were uncommon (≤16,7%), while in green pea, lupine, lentil, and bean-allergic patients co-allergy for peanut (64.7%–77.8%) or soybean (50%–64.7%) was frequently seen.

Conclusion

Co-sensitization between legumes was high, but generally not clinically relevant. Co-allergy to other legumes was not often seen in peanut- and soybean allergic patients. The 7S and 11S globulins were likely responsible for the observed co-sensitization.
Mechanical disturbances applied by motorized ankle foot orthosis to adapt ankle muscles activation—A validation study
Authors: Asín-Prieto Guillermo , Oliveira Barroso Filipe , Martínez-Expósito Aitor , Urendes Eloy , Gonzalez-Vargas Jose , Moreno Juan C.
Published: March 2023

Background: Reduced function of ankle muscles usually leads to impaired gait. Motorized ankle foot orthoses (MAFOs) have shown potential to improve neuromuscular control and increase volitional engagement of ankle muscles. In this study, we hypothesize that specific disturbances (adaptive resistance-based perturbations to the planned trajectory) applied by a MAFO can be used to adapt the activity of ankle muscles. The first goal of this exploratory study was to test and validate two different ankle disturbances based on plantarflexion and dorsiflexion resistance while training in standing still position. The second goal was to assess neuromuscular adaptation to these approaches, namely, in terms of individual muscle activation and co-activation of antagonists.

Methods: Two ankle disturbances were tested in ten healthy subjects. For each subject, the dominant ankle followed a target trajectory while the contralateral leg was standing still: a) dorsiflexion torque during the first part of the trajectory (Stance Correlate disturbance—StC), and b) plantarflexion torque during the second part of the trajectory (Swing Correlate disturbance—SwC). Electromyography was recorded from the tibialis anterior (TAnt) and gastrocnemius medialis (GMed) during MAFO and treadmill (baseline) trials.

Results: GMed (plantarflexor muscle) activation decreased in all subjects during the application of StC, indicating that dorsiflexion torque did not enhance GMed activity. On the other hand, TAnt (dorsiflexor muscle) activation increased when SwC was applied, indicating that plantarflexion torque succeeded in enhancing TAnt activation. For each disturbance paradigm, there was no antagonist muscle co-activation accompanying agonist muscle activity changes.

Conclusion: We successfully tested novel ankle disturbance approaches that can be explored as potential resistance strategies in MAFO training. Results from SwC training warrant further investigation to promote specific motor recovery and learning of dorsiflexion in neural-impaired patients. This training can potentially be beneficial during intermediate phases of rehabilitation prior to overground exoskeleton-assisted walking. Decreased activation of GMed during StC might be attributed to the unloaded body weight in the ipsilateral side, which typically decreases activation of anti-gravity muscles. Neural adaptation to StC needs to be studied thoroughly in different postures in futures studies.
Identification of circular RNAs expression pattern in caprine fetal fibroblast cells exposed to a chronic non-cytotoxic dose of graphene oxide-silver nanoparticle nanocomposites
Authors: Yuan Yu-Guo , Xing Yi-Tian , Liu Song-Zi , Li Ling , Reza Abu Musa Md Talimur , Cai He-Qing , Wang Jia-Lin , Wu Pengfei , Zhong Ping , Kong Il-Keun
Published: March 2023

The widespread use of graphene oxide-silver nanoparticle nanocomposites (GO-AgNPs) in biomedical sciences is increasing the chances of human and animal exposure to its chronic non-toxic doses. Exposure to AgNPs-related nanomaterials may result in the negative effect on the dam, fetus and offspring. However, there are only little available information for profound understanding of the epigenetic alteration in the cells and animals caused by low-dose chronic exposure of GO-AgNPs. The present study investigated the effect of 0.5 μg/mL GO-AgNPs for 10 weeks on the differential expression of circular RNAs (circRNAs) in caprine fetal fibroblast cells (CFFCs), and this dose of GO-AgNPs did not affect cell viability and ROS level. We predicted the functions of those differentially expressed (DE) circRNAs in CFFCs by bioinformatics analysis. Furthermore, we validated the expression of ten DE circRNAs using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) to ensure the reliability of the sequencing data. Our results showed that the DE circRNAs may potentially regulate the GO-AgNPs-inducing epigenetic toxicity through a regulatory network consisted of circRNAs, miRNAs and messenger RNAs (mRNAs). Therefore, the epigenetics toxicity is essential to assess the biosafety level of GO-AgNPs.
Single-cell transcriptome profiling reveals enriched memory T-cell subpopulations in hypertension
Authors: Wang Xiaoqi , Wu Xiaobin , Zhang Pei , Zhou Yuan , Cai Jun , Jin Ling
Published: March 2023

Introduction: The adaptive immune response mediated by T cells plays a vital role in the initiation and maintenance of blood pressure (BP) elevation. Memory T cells, which are antigen-specific T cells, can respond specifically to repeated hypertensive stimuli. Although the roles of memory T cells in animal models are well studied, their maintenance and functions in hypertensive patients are poorly understood.

Method: Here, we focused on the circulating memory T cells of hypertensive patients. By using single-cell RNA sequencing technology, subsets of memory T cells were identified. Differentially expressed genes (DEGs) and functional pathways were explored for related biological functions in each population of memory T cells.

Result and Discussion: Our study identified four subsets of memory T cells in the blood of hypertensive patients, with CD8 effector memory T (TEM) cells accounting for more cells and demonstrating more biological functions than CD4 TEM cells. CD8 TEM cells were further analyzed using single-cell RNA sequencing technology, and subpopulation 1 was demonstrated to contribute to BP elevation. The key marker genes CKS2, PLIN2, and CNBP were identified and validated by mass-spectrum flow cytometry. Our data suggest that CD8 TEM cells as well as the marker genes could be preventive targets for patients with hypertensive cardiovascular disease.
N6-methyladenosine modifications in maternal-fetal crosstalk and gestational diseases
Authors: Wu Suqi , Liu Ketong , Zhou Bingyan , Wu Suwen
Published: March 2023

As a medium among pregnant women, environment and fetus, placenta owns powerful and delicate epigenetic processes to regulate gene expression and maintain cellular homeostasis. N6-methyladenosine (m6A) is the most prevalent modification that determines the fate of RNA, and its dynamic reversibility indicates that m6A may serve as a sensitive responder to environmental stimuli. Emerging evidence suggests that m6A modifications play an essential role in placental development and maternal-fetal crosstalk, and are closely related to gestational diseases. Herein, we summarized the latest techniques for m6A sequencing and highlighted current advances of m6A modifications in maternal-fetal crosstalk and the underlying mechanisms in gestational diseases. Therefore, proper m6A modifications are important in placental development, but its disturbance mainly caused by various environmental factors can lead to abnormal placentation and function with possible consequences of gestational diseases, fetal growth and disease susceptibility in adulthood.
Synthesis of strong magnetic response ZIF-67 for rapid adsorption of Cu2+
Authors: Lei Yuanhang , Yang Haibo , Xie Jiangqin , Chen Qi , Quan Wenxuan , Wang Anping
Published: March 2023

With the acceleration of industrialization and urbanization, global water resources have been polluted. Among the water pollutants, heavy metals have caused great harm to the environment and organisms. When the concentration of Cu2+ in water exceeds the standard, the intake of the human body will mainly damage the nervous system. We use MOF materials with high chemical stability, specific surface area, adsorption, and other unique properties to adsorb Cu2+. MOF-67 was prepared with various solvents, and a stronger magnetic response MOF-67 with the largest surface area and best crystal form were selected. It quickly adsorbs low-concentration Cu2+ in water to purify water quality. At the same time, it can be recovered promptly through an external magnetic field to avoid secondary pollution, which conforms to the concept of green environmental protection. When the initial concentration of Cu2+ is 50 mg/L for 30 min, the adsorption rate reaches 93.4%. The magnetic adsorbent can be reused three times.
Brief review: Applications of nanocomposite in electrochemical sensor and drugs delivery
Authors: Haq Khan Zia Ul , Khan Taj Malook , Khan Amjad , Shah Noor Samad , Muhammad Nawshad , Tahir Kamran , Iqbal Jibran , Rahim Abdur , Khasim Syed , Ahmad Iftikhar , Shabbir Khadija , Gul Noor Shad , Wu Jianbo
Published: March 2023

The recent advancement of nanoparticles (NPs) holds significant potential for treating various ailments. NPs are employed as drug carriers for diseases like cancer because of their small size and increased stability. In addition, they have several desirable properties that make them ideal for treating bone cancer, including high stability, specificity, higher sensitivity, and efficacy. Furthermore, they might be taken into account to permit the precise drug release from the matrix. Drug delivery systems for cancer treatment have progressed to include nanocomposites, metallic NPs, dendrimers, and liposomes. Materials’ mechanical strength, hardness, electrical and thermal conductivity, and electrochemical sensors are significantly improved using nanoparticles (NPs). New sensing devices, drug delivery systems, electrochemical sensors, and biosensors can all benefit considerably from the NPs’ exceptional physical and chemical capabilities. Nanotechnology is discussed in this article from a variety of angles, including its recent applications in the medical sciences for the effective treatment of bone cancers and its potential as a promising option for treating other complex health anomalies via the use of anti-tumour therapy, radiotherapy, the delivery of proteins, antibiotics, and vaccines, and other methods. This also brings to light the role that model simulations can play in diagnosing and treating bone cancer, an area where Nanomedicine has recently been formulated. There has been a recent uptick in using nanotechnology to treat conditions affecting the skeleton. Consequently, it will pave the door for more effective utilization of cutting-edge technology, including electrochemical sensors and biosensors, and improved therapeutic outcomes.
Comparative proteomics analysis of adult Haemonchus contortus isolates from Ovis ammon
Authors: Liu Gongzhen , Liu Qing , Han Zhaoqing , Wang Peikun , Li Yanshen
Published: March 2023

Haemonchus contortus is an important parasite that causes disease that seriously endangers ruminant animals cattle, sheep, goat, and camel. Here, we compared the proeomics analysis of three adult Haemonchus contortus isolates from mouflons (Ovis ammon). A total of 1,299 adult worm proteins were identified, and 461 proteins were quantified, of which 82 (108), 83 (97), and 97 (86) significantly upregulated (downregulated) differentially expressed proteins (DEPs) were detected among pairwise comparisons (1-vs.-3, 2-vs.-3, and 2-vs.-1). Liquid chromatography–tandem mass spectrometry (LC−MS/MS) and bioinformatic analysis indicated that these DEPs are mainly concentrated in cellular composition, molecular function, biological function, and catabolism pathways. In addition, Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out to screen the DEPs. The main biological processes involved were nucleotide, nucleotide phosphate, ribonucleotide, purine-containing compound, purine ribonucleotide, single-organism, oxoacid, organic, carboxylic, oxoacid metabolic processes and single-organism catabolic processes. The majority of KEGG pathways were found to be related to metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, carbon metabolism, and microbial metabolism in diverse environments. Moreover, we also found differences in the expression of some important or novel regulatory proteases, such as serine hydroxymethyl transferase (SHMT), dihydrolipoyl dehydrogenase (DLD), and transket pyr domain-containing protein (TKPD). In summary, label-free proteomic analysis of adult H. contortus worms displayed significant differences in three different individual isolates, which helps to improve our understanding of the growth and metabolic mechanisms of H. contortus in different individuals and relative natural environments and provides novel drug targets for the treatment of parasitic diseases.
Online corporate communication: Should national culture matter?
Authors: Capece Guendalina , Di Pillo Francesca
Published: March 2023

Introduction

The purpose of this paper is to investigate how the culture of a nation influences online corporate communication, focusing on the assessment of the culturability of business websites. Although the Internet constitutes a global phenomenon, cultural filters influence its use at the local level, ultimately determining a more or less favorable attitude toward a given website. Understanding and analysing the cultural adaptation of online communication is crucial as it has the potential to greatly influence how customers perceive and respond to the graphic and content elements.

Methods

Building upon Hofestede's and Hall's theories, a content analysis technique was used to code the cultural markers and new indicators have been created to allow the association of the graphic elements and the contents of the websites with the cultural characteristics. The countries chosen as case studies are India and Australia, which present significant cultural differences and allow highlighting of the practical implications deriving from the cultural adaptation of the website design.

Results

The results of this paper show that the company websites analyzed are designed to incorporate the cultural nuances of the target country. In particular, on the Australian websites, there is a greater frequency of cultural markers referring to individualism, masculinity, and uncertainty avoidance compared to Indian ones. On the contrary, Indian websites show more cultural markers referring to power distance, high context, and polychronic characteristics than Australian ones. This paper overcomes the qualitative approaches of the previous literature, developing new indicators for website analysis and providing a quantitative comparison with Hofstede and Hall frameworks.

Discussion

This work provides a guideline for companies, analysts, and the different professionals involved in online communication and web design. More specifically, they have to be aware of the cultural challenges when they operate outside the national boundaries, by designing a culturally usable website in one of the countries chosen for this study.
Improved computation of Lagrangian tissue displacement and strain for cine DENSE MRI using a regularized spatiotemporal least squares method
Authors: Ghadimi Sona , Abdi Mohamad , Epstein Frederick H.
Published: March 2023

Introduction

In displacement encoding with stimulated echoes (DENSE), tissue displacement is encoded in the signal phase such that the phase of each pixel in space and time provides an independent measurement of absolute tissue displacement. Previously for DENSE, estimation of Lagrangian displacement used two steps: first a spatial interpolation and, second, least squares fitting through time to a Fourier or polynomial model. However, there is no strong rationale for such a through-time model,

Methods

To compute the Lagrangian displacement field from DENSE phase data, a minimization problem is introduced to enforce fidelity with the acquired Eulerian displacement data while simultaneously providing model-independent regularization in space and time, enforcing only spatiotemporal smoothness. A regularized spatiotemporal least squares (RSTLS) method is used to solve the minimization problem, and RSTLS was tested using two-dimensional DENSE data from 71 healthy volunteers.

Results

The mean absolute percent error (MAPE) between the Lagrangian displacements and the corresponding Eulerian displacements was significantly lower for the RSTLS method vs. the two-step method for both x- and y-directions (0.73±0.59 vs 0.83 ±0.1, p < 0.05) and (0.75±0.66 vs 0.82 ±0.1, p < 0.05), respectively. Also, peak early diastolic strain rate (PEDSR) was higher (1.81±0.58 (s-1) vs. 1.56±0. 63 (s-1), p<0.05) and the strain rate during diastasis was lower (0.14±0.18 (s-1) vs 0.35±0.2 (s-1), p < 0.05) for the RSTLS vs. the two-step method, with the former suggesting that the two-step method was over-regularized.

Discussion

The proposed RSTLS method provides more realistic measurements of Lagrangian displacement and strain from DENSE images without imposing arbitrary motion models.
Evaluation of the safety and efficacy of a Polyzene-F nanocoated coronary stent system: A systematic review and single-arm meta-analysis
Authors: Bian Jifang , Yang Rongyuan , Wang Dawei , Yu Huimin , Liu Yuan , Liu Qing
Published: March 2023

Background

A stent for patients with coronary heart disease (CHD) provides a requirement for a long-term antiplatelet therapy because of the high possibility of the development of stent thrombosis. It was against this background that both Cobra and Catania Polyzene-F (PzF) stents were designed to reduce the occurrence of stent thrombosis (ST). In this study, we review the safety and effectiveness of a PzF-nanocoated stent.

Methods

This systematic review with the title was registered in PROSPERO (No.398781). The inclusion criteria were including studies among patients with PzF-nanocoated coronary stents and reported target vessel failure (TVF) and ST as the outcomes, and the exclusion criteria were excluding reported patients who could not receive the adjunctive medical therapies or without the necessary endpoints. Reports about PzF-nanocoated stents were searched in PubMed, Embase, and Web of Science and other sources. Because of the existence of few reports and a lack of comparison groups, a single-arm meta-analysis was conducted in R software (v3.6.2), using a random-effects model with the generic inverse variance method. After a heterogeneity test, assessment of evidence quality was conducted by using GRADE software. A funnel plot Egger's test was performed to evaluate publication bias, and a sensitivity analysis was done to determine the robustness of the overall effects.

Results

Six studies of 1,768 subjects were included. The primary endpoint that pooled the TVF rate was 8.9% (95% CI 7.5%–10.2%), which comprised the pooled cardiac death (CD) rate (1.5%, 95% CI 0%–3%), myocardial infarction (MI) rate (2.7%, 95% CI 0.4%–5.1%), target vessel revascularization (TVR) (4.8%, 95% CI 2.4%–7.2%), or target lesion revascularization (TLR) (5.2%, 95% CI 4.2%–6.4%), while the secondary endpoint ST was 0.4% (95% CI 0.1%–0.9%). The funnel plots of TVF, CD, TVR, and TLR did not show any serious publication bias, and TVF, TVR, and TLR showed evidence of moderate quality in GRADE assessment. The sensitivity analysis showed that TVF, TLR, and ST exhibited good stability (I2 = 26.9%, 16.4%, and 35.5%, respectively), while the other endpoints showed moderate instability.

Conclusion

These data indicated that the PzF-nanocoated coronary stents of the Cobra and Catania systems demonstrated good safety and efficacy in clinical application. However, the sample size of patients included in the reports was relatively small, and this meta-analysis will be updated if more studies are published in the future.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42023398781