bluuebunny/crossref_metadata_2025 huggingface dataset:
N-Glycomic Profiling of Microsatellite Unstable Colorectal Cancer
Authors: Ukkola Iiris , Nummela Pirjo , Heiskanen Annamari , Holm Matilda , Zafar Sadia , Kero Mia , Haglund Caj , Satomaa Tero , Kytölä Soili , Ristimäki Ari
Published: July 2023

Aberrant glycosylation affects cancer progression and immune evasion. Approximately 15% of colorectal cancers (CRCs) demonstrate microsatellite instability (MSI) and display major differences in outcomes and therapeutic responses, as compared to corresponding microsatellite stable (MSS) tumors. We compared the N-glycan profiles of stage II and IV MSI CRC tumors, further subdivided into BRAFV600E wild-type and mutated subgroups (n = 10 in each subgroup), with each other and with those of paired non-neoplastic mucosal samples using mass spectrometry. Further, the N-glycans of BRAFV600E wild-type stage II MSI tumors were compared to corresponding MSS tumors (n = 9). Multiple differences in N-glycan profiles were identified between the MSI CRCs and control tissues, as well as between the stage II MSI and MSS samples. The MSI CRC tumors showed a lower relative abundance of high-mannose N-glycans than did the control tissues or the MSS CRCs. Among MSI CRC subgroups, acidic N-glycans showed tumor stage and BRAF mutation status-dependent variation. Specifically, the large, sulfated/phosphorylated, and putative terminal N-acetylhexosamine-containing acidic N-glycans differed between the MSI CRC subgroups, showing opposite changes in stages II and IV, when comparing BRAF mutated and wild-type tumors. Our results show that molecular subgroups of CRC exhibit characteristic glycan profiles that may explain certain carcinogenic properties of MSI tumors.
Neural Progenitor Cells and the Hypothalamus
Authors: Makrygianni Evanthia A. , Chrousos George P.
Published: July 2023

Neural progenitor cells (NPCs) are multipotent neural stem cells (NSCs) capable of self-renewing and differentiating into neurons, astrocytes and oligodendrocytes. In the postnatal/adult brain, NPCs are primarily located in the subventricular zone (SVZ) of the lateral ventricles (LVs) and subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). There is evidence that NPCs are also present in the postnatal/adult hypothalamus, a highly conserved brain region involved in the regulation of core homeostatic processes, such as feeding, metabolism, reproduction, neuroendocrine integration and autonomic output. In the rodent postnatal/adult hypothalamus, NPCs mainly comprise different subtypes of tanycytes lining the wall of the 3rd ventricle. In the postnatal/adult human hypothalamus, the neurogenic niche is constituted by tanycytes at the floor of the 3rd ventricle, ependymal cells and ribbon cells (showing a gap-and-ribbon organization similar to that in the SVZ), as well as suprachiasmatic cells. We speculate that in the postnatal/adult human hypothalamus, neurogenesis occurs in a highly complex, exquisitely sophisticated neurogenic niche consisting of at least four subniches; this structure has a key role in the regulation of extrahypothalamic neurogenesis, and hypothalamic and extrahypothalamic neural circuits, partly through the release of neurotransmitters, neuropeptides, extracellular vesicles (EVs) and non-coding RNAs (ncRNAs).
An Experimental Approach to Estimation of the Energy Cost of Dynamic Branch Prediction in an Intel High-Performance Processor
Authors: Alqurashi Fahad Swilim , Al-Hashimi Muhammad
Published: July 2023

Power and energy efficiency are among the most crucial requirements in high-performance and other computing platforms. In this work, extensive experimental methods and procedures were used to assess the power and energy efficiency of fundamental hardware building blocks inside a typical high-performance CPU, focusing on the dynamic branch predictor (DBP). The investigation relied on the Running Average Power Limit (RAPL) interface from Intel, a software tool for credibly reporting the power and energy based on instrumentation inside the CPU. We used well-known microbenchmarks under various run conditions to explore potential pitfalls and to develop precautions to raise the precision of the measurements obtained from RAPL for more reliable power estimation. The authors discuss the factors that affect the measurements and share the difficulties encountered and the lessons learned.
Correction: Alshehri et al. Unsteady Convective MHD Flow and Heat Transfer of a Viscous Nanofluid across a Porous Stretching/Shrinking Surface: Existence of Multiple Solutions. Crystals 2021, 11, 1359
Authors: Alshehri Nawal A. , Abidi Awatef , Khan Muhammad Riaz , Reddy Yanala Dharmendar , Rasheed Saim , Alali Elham , Galal Ahmed M.
Published: July 2023

We ought to correct the misprinted terms in our published paper ‘Unsteady Convective MHD Flow and Heat Transfer of a Viscous Nanofluid across a Porous Stretching/Shrinking Surface: Existence of Multiple Solutions’ [Crystals2021, 11, 1359] [...]
The Case for a Nuclear Barcode: Using the CAD CPS Region for Species and Genus Level Discrimination in Beetles
Authors: Gorring Patrick S. , Cognato Anthony I.
Published: July 2023

DNA barcoding has revolutionized how we discover, identify, and detect species. A substantial foundation has been established with millions of mitochondrial cytochrome c oxidase I sequences freely available for eukaryotes. However, issues with COI ranging from uniparental inheritance and small genetic population sizes to nuclear and asymmetric introgression can impede its use. We propose using CAD as the “nuclear barcode” to complement the COI barcode and ameliorate these concerns. We focused on beetles from taxonomically diverse species-level studies that used COI and CAD. An ambiguous barcode gap was present between intra- and interspecific genetic distances in CAD and COI; this led to difficulty with automated gap detection methods. We found pseudogenes, problematic population structure, introgression, and incomplete lineage sorting represented in the COI data. A CAD gene tree illuminated these cryptic problems. Placement tests of species and outgroups using distance-based tree building were largely successful for CAD, demonstrating its phylogenetic signal at the species and genus levels. Species placement issues were typically unique to one locus, allowing for recognition of misdiagnosis. We conclude that a CAD barcode is a valuable tool for beetle diagnostics, metabarcoding, and faunistic surveys.
pELECTRE-Tri: Probabilistic ELECTRE-Tri Method—Application for the Energy Renovation of Buildings
Authors: Baseer Majid , Ghiaus Christian , Viala Roxane , Gauthier Ninon , Daniel Souleymane
Published: July 2023

This paper proposes the method pELECTRE Tri, a novel probabilistic Multi-Criteria Decision Making (MCDM) method using the traditional ELECTRE Tri, probability distribution, and Monte Carlo simulation to support informed decision-making in complex and uncertain decision environments. The proposed method is illustrated through a case study involving the renovation of three social housing buildings. The paper provides new insights into the application of probabilistic sorting MCDM in the context of energy efficiency in buildings and highlights the benefits of using probabilities rather than crisp values to categorize alternatives enabling stakeholders to make better use of available resources, especially when dealing with a large dataset of energy measures with different features. The methodology implemented in Python (DOI: 10.5281/zenodo.7967655) is available as an open source.
Application of Particle Swarm Optimization (PSO) Algorithm in Determining Thermodynamics of Solid Combustibles
Authors: Pan Haoyu , Gong Junhui
Published: July 2023

The thermodynamics of a solid are crucial in predicting thermal responses and fire behaviors, and they are commonly determined by inverse modeling and optimization algorithms at constant heat flux. However, in practical scenarios, the imposed heat flux frequently varies with time, and related thermodynamics determination methods are rarely reported. In this study, the particle swarm optimization (PSO) algorithm and a 1D numerical model were utilized to determine temperature-dependent thermal conductivity and specific heat of beech wood and polymethyl methacrylate (PMMA). Surface, 3 and 6 mm in-depth temperatures were measured in three sets of ignition tests where constant and time-dependent heat fluxes (HFs) were applied. In each set, PSO was implemented at individual HFs, and the average value was deemed as the final outcome. Reliability of the optimized thermodynamics was verified by comparing with the reported values in the literature and predicting the experimental measurements that were not employed during parameterization. The results showed that wood thermodynamics attained under constant and time-dependent HFs in agreement with previously reported ones. Similar optimization procedures were conducted for PMMA, and good agreement with literature values was found. Using the obtained thermodynamics of wood under constant HF, the numerical model successfully captured the surface temperature at time-dependent HFs. Meanwhile, comparisons using wood temperatures at constant HFs and PMMA temperatures at linear HFs also verified the feasibility of PSO.
Looking for an Optimal Composition of Nickel-Based Catalysts for CO2 Methanation
Authors: Busca Guido , Spennati Elena , Riani Paola , Garbarino Gabriella
Published: July 2023

A detailed critical analysis of the scientific literature data concerning catalysts for CO2 methanation based on nickel supported over oxides was performed. According to the obtained information, it seems that an ionic support is necessary to allow a good nickel dispersion to produce very small nickel metal particles. Such small metal particles result in being very active toward methanation, limiting the production of carbonaceous materials. The use of support and/or surface additives gives rise to medium surface basicity, allowing medium-strong adsorption of CO2, and it is also advisable to increase the reaction rate. A medium nickel loading would allow the free support geometric surface to be covered densely by small nickel metal particles without the production of larger Ni crystals. It is also advisable to work at temperatures where Ni(CO)4 formation is not possible (e.g., >573 K). The promising properties of systems based on doped Ni/Al2O3, doped with basic and re-active oxides such as MnOx or/and CeO2, and those based on Ni/CeO2 were underlined.
Dominance of Topography on Vegetation Dynamics in the Mt. Qomolangma National Nature Reserve: A UMAP and PLS-SEM Analysis
Authors: Xu Binni , Li Jingji , Pei Xiangjun , Bian Lijiao , Zhang Tingbin , Yi Guihua , Bie Xiaojuan , Peng Peihao
Published: July 2023

The southern portion of the Qinghai–Tibet Plateau (QTP) and the central Himalayan region are home to the Mt. Qomolangma (Everest) National Nature Reserve (QNNR), which is the world’s highest nature reserve and is distinguished by delicate natural ecosystems and unique geographic features. Analyzing regional vegetation trends, as well as the impacts of natural and anthropogenic variables on vegetation coverage, is crucial for local environmental protection and sustainable development. In this study, the variation patterns of the MOD13Q1 Normalized Difference Vegetation Index (NDVI) data were explored, and the responses of vegetation development to both natural and anthropogenic parameters were investigated by applying trend analysis and partial correlation analysis, as well as the partial least squares-structural equation model (PLS-SEM). To better comprehend the spatial characteristics and interrelationships between NDVI and various parameters under different vegetation types, the Uniform Manifold Approximation and Projection (UMAP) was employed for dimensionality reduction and visualization. The results illustrated that between 2000 and 2018, the reserve greened up at a rate of 0.00073/a (p < 0.05), with vegetation improvement areas accounting for 49.46%. The major climatic driver for the greening trend of vegetation was temperature. Topography (especially elevation) remains dominant in regulating vegetation development in the QNNR, despite a progressively growing impact of hydrothermal conditions on vegetation development. Additionally, the implementation of environmental initiatives has stifled the adverse impacts of human activity.
Forest Canopy Water Content Monitoring Using Radiative Transfer Models and Machine Learning
Authors: Liu Liang , Li Shaoda , Yang Wunian , Wang Xiao , Luo Xinrui , Ran Peilian , Zhang Helin
Published: July 2023

Forests are facing various threats, such as drought, in the context of global climate change. Canopy water content (CWC) is a crucial indicator of forest water stress, mortality, and fire monitoring. However, previous studies on CWC have not adequately simulated forests with heterogeneous and discontinuous canopy structures. At the same time, there is a lack of field validation. This study retrieved the forest CWC across the contiguous U.S. (CONUS) with coupled radiative transfer models (RTMs) and the random forest (RF) algorithm. A Gaussian copula and prior knowledge were used for model parameterization. The results indicated that more accurate simulations of leaf trait dependencies and canopy structure characteristics lead to better CWC inversion. In addition, GeoSail, coupled with PROSPECT-5B, showed good performance (R2 = 0.68, RMSE = 0.15 kg m−2, MAE = 0.12 kg m−2, rRMSE = 12.78%, Bias = −0.036 kg m−2) for forest CWC retrieval. Large variation existed in forest CWC, spatiotemporally, and evergreen needle forest (ENF) showed strong CWC capacity. This study underscores the suitability of 3D RTMs for inversing the parameters of forest canopies.
Field-Measured Hydraulic Traits and Remotely Sensed NDVI of Four Subtropical Tree Species Showed Transient Declines during the Drought–Heatwave Event
Authors: Wang Yongkang , Song Jia
Published: July 2023

Unpredictable drought–heatwave events occur frequently worldwide, causing low water availability (drought) and high temperatures (hot), with consequences for forest decline and mortality. Our knowledge of the potential instantaneous reactions and subsequent recovery of water-related physiological processes and vegetation indices in hot drought events remains unclear. Here, we investigated how the 2022 summer drought–heatwave event in the subtropical regions of China affected hydraulic traits and NDVI values in the forests of four common subtropical tree species. During the hot drought, the NDVI values of all four forests decreased (−31%~−23%), accompanied by leaf scorch and tree crown dieback. Among the four species, a hot drought event caused an instantaneous descent in hydraulic conductivity (Ks, −72%~−31%), stomatal conductance (gs, −94%~−50%), and midday water potential (−40%~−169%), with severe drought-induced stem xylem embolism. A trade-off was found between resistance and resilience in hot-drought-induced hydraulic dysfunction, as species with lower declines in Ks and gs during the hot drought had a shorter recovery in the post-stress phase. This study highlights that the 2022 hot drought event had severe negative instantaneous impacts on the forests of four subtropical tree species, which were reflected both in water-related physiological processes in the field and in remote sensing data from satellites.
Construction of Eicosatetraenoic Acid Producing Cell Factory by Genetic Engineering of Mucor circinelloides
Authors: Wu Chen , Yang Junhuan , Li Shaoqi , Shi Wenyue , Xue Futing , Liu Qing , Naz Tahira , Mohamed Hassan , Song Yuanda
Published: July 2023

Eicosatetraenoic acid (ETA, 20:4, ω-3) is the desaturation product of dihomo-gamma linolenic acid (DGLA, 20:3, ω-6) catalyzed by delta-17 desaturase, which is considered as a healthy product that helps to lower risk of heart diseases. The oleaginous filamentous fungus, Mucor circinelloides, has been used for a long time as a model micro-organism for GLA production at industrial scales. However, M. circinelloides lacks the key enzymes to synthesize C20 polyunsaturated fatty acids (PUFAs). M. circinelloides could produce DGLA by overexpressing the D6E(GLELO) gene, which could be a useful tool to produce ETA due to the availability of established genetic manipulation tools. Therefore, in this study, delta-17 desaturase (PpD17 and PaD17) genes from Phytophthora parasitica and Pythium aphanidermatum, respectively, were introduced into M. circinelloides to construct an ETA-producing cell factory. Our results showed that the PaD17 and PpD17 overexpression strains’ biomass increased by 25.98 and 23.34 g/L (39.98 and 25.75%), respectively, compared with the control strain. Meanwhile, the lipid contents of the recombinant strains also increased and reached up to 28.88% in Mc-PaD17 and 30.95% in Mc-PpD17, respectively, compared with the control strain (23.38% in Mc-2076). The RT-qPCR results showed that overexpression of delta-17 desaturase genes promoted the expression of cme2, fas2, and D6E, thereby contributing to lipid biosynthesis in M. circinelloides. Meanwhile, the content of ETA reached up to 1.95%, and the yield of ETA was up to 114.69 mg/L in PpD17 overexpression mutants at 96 h. This study provided the first report on the construction of an ETA-producing cell factory by heterologous overexpression of the PpD17 gene in M. circinelloides, which established a new scope for further research in the production of ETA in oleaginous fungi.
An Updated Review of Soy-Derived Beverages: Nutrition, Processing, and Bioactivity
Authors: Olías Raquel , Delgado-Andrade Cristina , Padial María , Marín-Manzano M. Carmen , Clemente Alfonso
Published: July 2023

The global market for plant-based drinks is experiencing rapid growth driven by consumer demand for more sustainable diets, including vegetarian and vegan options. Soy beverages in particular are gaining popularity among individuals with lactose intolerance and milk protein allergies. They are considered an excellent source of high-quality protein, vitamin B, unsaturated fatty acids, and beneficial phytochemicals such as phytosterols, soy lecithins, and isoflavones. This review presents a comprehensive market survey of fifty-two soy beverages available in Spain and other European countries. The predominant category among those evaluated was calcium and vitamin-fortified drinks, accounting for 60% of the market. This reflects the need to address the nutritional gap compared to cow’s milk and meet essential dietary requirements. The review covers the technological aspects of industrial soy milk production, including both traditional methods and innovative processing techniques. Additionally, it analyzes multiple studies and meta-analyses, presenting compelling evidence for the positive effects of soy beverages on various aspects of health. The review specifically examines the contributions of different components found in soy beverages, such as isoflavones, proteins, fiber, and oligosaccharides. Moreover, it explores controversial aspects of soy consumption, including its potential implications for growth, puberty, fertility, feminization, and the thyroid gland.
Effect of Fertilization Regime of Common Wheat (Triticum aestivum) on Flour Quality and Shelf-Life of PDO Tuscan Bread
Authors: Bianchi Alessandro , Sanmartin Chiara , Taglieri Isabella , Macaluso Monica , Venturi Francesca , Napoli Marco , Mancini Marco , Fabbri Carolina , Zinnai Angela
Published: July 2023

The shelf-life of bread is influenced by flour components, such as starch, composed of amylose and amylopectin. The aim was to test the effect of different balances of N (45, 90, 135 kg/ha) and P (48, 96 kg/ha) fertilizers on the flour characteristics and consequently the shelf-life of PDO Tuscan bread, stored in different modified atmosphere packaging (Ar, N2, Air). The amylose and phytochemical compounds were increased by N and decreased by the addition of P, but excessive doses of N (135 kg/ha) had a negative effect on flour quality. In the bread, the study highlighted the tendency of N2 and Ar, as storage filler gases, to reduce water loss, slow down the staling process, and prolong shelf-life. However, the most significant influence on shelf-life was related to the different fertilizations of wheat. In fact, when N was present in equal dose to P (90/96 or 45/48 kg/ha) or slightly higher (90/48 kg/ha), the bread tended to last longer over time. Instead, when these ratios were unbalanced in favor of N (135/48 or 135/96 kg/ha) and in favor of P (45/96 kg/ha), the shelf-life decreased considerably.
Quality Attributes and Sensory Acceptance of Different Botanical Coffee Co-Products
Authors: Poláková Katarína , Bobková Alica , Demianová Alžbeta , Bobko Marek , Lidiková Judita , Jurčaga Lukáš , Belej Ľubomír , Mesárošová Andrea , Korčok Melina , Tóth Tomáš
Published: July 2023

Coffee processing is a major contributor to the creation of food and product waste. Using coffee co-products can play an essential role in addressing environmental problems and issues with nutritionally unbalanced foods, population growth, and food-related diseases. This research aimed to determine the quality and sensory parameters (aw, pH, dry matter, TAC, TPC, fat, fatty acids profile, fiber, caffeine, chlorogenic acids, color, and sensory analysis) of different botanical origins of cascara (coffee husks) and silverskin (thin layer). The results of this study show that silverskin and cascara are a good source of TAC (1S 58.17 ± 1.28%, 2S 46.65 ± 1.20%, 1C 36.54 ± 1.84%, 2C 41.12 ± 2.11%). Cascara showed the presence of polyphenols (2C 49.135 g GAE·kg−1). Coffee co-products are good sources of fiber. Silverskin had higher values of caffeine than cascara. Palmitic, stearic, oleic, linoleic, and arachidic acids were the most represented acids in the samples. Given the obtained results, cascara can be considered “low-fat” (1C 4.240 g·kg−1 and 2C 5.4 g·kg−1). Based on the sensory evaluation, no sample reached the acceptable index value of 70%. Understanding the link between the character, identification properties, and composition of coffee co-products of different botanical origins can enable their application in the food industry.
Physicochemical, Structural, and Functional Properties of Snake Melon (Cucumis melo subsp. melo Var. flexuosus) Microencapsulated with Pea Protein and Pea Fibre by Freeze-Drying
Authors: Igual Marta , Flores-León Alejandro , Picó Belén , Martínez-Monzó Javier , García-Segovia Purificación
Published: July 2023

The purpose of this study was to obtain a functional, stable powder product from Cucumis melo subsp. melo Var. flexuosus (L.) to promote its consumption and reduce waste and production losses. The melons were ground and freeze-dried with or without biopolymers (pea protein (PPSM) or pea fibre (PFSM)). The physicochemical, structural, and functional properties of the powder were studied. The water content, water activity, bulk density, porosity, Hausner ratio, Carr index, hygroscopicity, water solubility, water absorption index, particle size, colour, and microstructure of the powder were determined. In addition, vitamin C, folates, chlorophyll a, total phenols and carotenoids, antioxidant capacity, and powder encapsulation efficiency were analysed. Snake melon (SM) powders contained vitamin C, folates, carotenoids, chlorophyll a, and phenols, which contributed to their antioxidant capacity. The incorporation of PP or PF in the formulation before lyophilisation generated stable encapsulates that protected the bioactive compounds. PPSM and PFSM were less hygroscopic and more free-flowing and had lower water content and water activity compared to the SM. PFSM showed higher encapsulation efficiency and smaller particles with a smooth surface and oval shape.
The Characteristics of Whey Protein and Blueberry Juice Mixed Fermentation Gels Formed by Lactic Acid Bacteria
Authors: Wang Wenqiong , Wang Yuxian , Liu Xian , Yu Qian
Published: July 2023

The properties of blueberry juice and whey protein gels formed by the mixed fermentation of L. plantarum 67 and L. paracasei W125 were investigated. The state of the gels, including the colour and surface morphology of the microspheres, showed significant changes with different fermentation times. The polyphenolic, flavonoid, and protein release of whey protein or combined blueberry juice fermented gels under in vitro digestion were investigated. The whey protein and blueberry juice fermented gels had more small pores, with a honeycomb structure, compared to whey protein fermented gels. The hardness of the gels was increased after fermentation for 7 h for the whey protein gels and whey protein mixture blueberry juice gels. The storage modulus and water-holding capacity of the gels were increased between fermentation times of 6 h and 8 h. The swelling rates of the whey protein gels fermented for 7 h and whey protein mixed blueberry juice gels fermented for 8 h and kept in pepsin-free simulated gastric fluid for 1 h had higher values. The release of polyphenols, flavonoids, and protein for the fermented gels was higher at fermentation of 7 h in the in vitro digestion experiment. We found that the chewiness of the whey protein gels, or whey protein mixed fermentation gels, was higher at a fermentation time of 7.5 h and 8 h. However, the cohesiveness values were not significantly different. Therefore, whey protein fermented gels and whey protein mixed blueberry juice fermented gels should be fermented for more than 7 h. This facilitates the release of polyphenols, flavonoids, and protein in the gastric juices.
Non-Coding RNAs in Human Health and Diseases
Authors: Good Deborah J.
Published: July 2023

Non-coding RNAs (ncRNAs) are, arguably, the enigma of the RNA transcriptome. Even though there are more annotated ncRNAs (25,967) compared to mRNAs (19,827), we know far less about each of the genes that produce ncRNA, especially in terms of their regulation, molecular functions, and interactions. Further, we are only beginning to understand the role of differential regulation or function of ncRNAs caused by genetic and epigenetic perturbations, such as single nucleotide variants (SNV), deletions, insertions, and histone/DNA modifications. The 22 papers in this Special Issue describe the emerging roles of ncRNAs in neurological, cardiovascular, immune, and hepatic systems, to name a few, as well as in diseases such as cancer, Prader–Willi Syndrome, cardiac arrhythmias, and diabetes. As we begin to understand the function and regulation of this class of RNAs, strategies targeting ncRNAs could lead to improved therapeutic interventions for some conditions.
The Italian Family Satisfaction in the Intensive Care Unit Questionnaire: A Psychometric Evaluation Using the Rasch Model
Authors: Danielis Matteo , Zanotti Renzo , Rosset Marika , Giorgino Serena , Gentilini Sara , Molaro Dina , Qualizza Anna , Garau Alessandro
Published: July 2023

Quality measurement of the intensive care unit (ICU) should include families’ perspectives, their satisfaction with the care process and outcomes, and the evaluation of actions to improve their psychological health and wellbeing. The current study was designed to validate the Italian version of the Family Satisfaction in the Intensive Care Unit (FS-ICU) using the Rasch model. Results included reliability and separation for items and persons, item fit statistics, unidimensionality, and item characteristic curve. The study was conducted between August 2022 and February 2023. A total of 108 family members (mean age 54.9 years) completed the FS-ICU questionnaire. The instrument had a moderate discrimination ability and only five items (#21, #23, #10, #22, and #24) exhibited a misfit. The Rasch dimension explained 52.1% of the variance in the data, while the unexplained variance in the first contrast is 7.2%, which indicates a possible second dimension. FS-ICU was shown to be beneficial as an assessment instrument for family member satisfaction in the ICU, despite some flaws that need to further be addressed to improve the scale.
Production of Tetraploid Plants from Cotyledons of Diploid Melia volkensii Gürke
Authors: Dushimimana Constantin , Van Laere Katrijn , Magomere Titus , Smagghe Guy , Werbrouck Stefaan P. O.
Published: July 2023

Polyploidy was induced in Melia volkensii (Mukau), a valuable native tree from the semi-arid regions of East Africa. Cotyledons of diploid M. volkensii (2n = 2x = 28) were treated with oryzalin for 0 (control), 1, 2, or 3 h with or without pretreatment with 1.1 µM thidiazuron. Cotyledons treated with 10 mg·L−1 oryzalin for three hours yielded 40% tetraploids. Pretreatment of cotyledons with thidiazuron for 18 days followed by treatment with oryzalin increased tetraploid plant production to 52.5%, but this also yielded more mixoploids. Compared to diploid M. volkensii, the tetraploid in vitro and young potted plants were compacter, with thicker stems, wider leaves, and a low density of longer and wider stomata. In the coming years, tetraploid M. volkensii plants will be observed in field trials and serve as a basis for further breeding efforts.
Do Interlimb Knee Joint Loading Asymmetries Persist throughout Stance during Uphill Walking Following Total Knee Arthroplasty?
Authors: Thorsen Tanner , Wen Chen , Porter Jared , Reinbolt Jeffery A. , Weinhandl Joshua T. , Zhang Songning
Published: July 2023

The purpose of this study was to determine differences in total (TCF), medial compartment (MCF), and lateral compartment (LCF) tibiofemoral joint compressive forces and related muscle forces between replaced and non-replaced limbs during level and uphill walking at an incline of 10°. A musculoskeletal modeling and simulation approach using static optimization was used to determine the muscle forces and TCF, MCF, and LCF for 25 patients with primary TKA. A statistical parametric mapping repeated-measures ANOVA was conducted on knee compressive forces and muscle forces using statistical parametric mapping. Greater TCF, MCF, and LCF values were observed throughout the loading response, mid-stance, and late stance during uphill walking. During level walking, knee extensor muscle forces were greater throughout the first 50% of the stance during level walking, yet greater during uphill walking during the last 50% of the stance. Conversely, knee flexor muscle forces were greater through the loading response and push-off phases of the stance. No between-limb differences were observed for compressive or muscle forces, suggesting that uphill walking may promote a more balanced loading of replaced and non-replaced limbs. Additionally, patients with TKA appear to rely on the hamstrings muscle group during the late stance for knee joint control, thus supporting uphill walking as an effective exercise modality to improve posterior chain muscle strength.
Trophic Chain Organochlorine Pesticide Contamination in a Highly Productive Upwelling Area in Southeastern Brazil
Authors: Lavandier Ricardo , Arêas Jennifer , Lemos Leila , de Moura Jailson , Taniguchi Satie , Montone Rosalinda , Quinete Natalia , Hauser-Davis Rachel , Siciliano Salvatore , Moreira Isabel
Published: July 2023

Organochlorine pesticides (OCP) are legacy anthropogenic compounds known to persist for several years in the environment. The continuous use of some OCP, such as DDT, after restrictions in developing countries are cause of concern, due to their deleterious effects to marine life and humans. Studies assessing OCP contamination in coastal environments are still scarce in South America and there is a need to understand the impacts from trophic chain accumulation of these pollutants in marine life. In this study, we have assessed OCP levels in muscle and liver and estimated the biomagnification factor in several upwelling system trophic chain members, including fish, squid, and marine mammal from Southeastern Brazil. DDT degradation product DDE was the OCP detected in the highest concentrations in Franciscana dolphins (Pontoporia blainvillei), 86.4 ng·g−1 wet weight, and fish muscle and liver. In general, higher OCP levels were found in liver than in muscle, except for croaker. Biomagnification factors (BMF) of OCP in the top predator P. blainvillei and the carnivorous cutlass fish (Trichiurus lepturus) were on average between 0.2 and 1.8. Continued OCP monitoring in this region is warranted to better understand the distribution and fate of these compounds over time, with the goal to establish strategies for the conservation of local dolphin species and to assess human health risks from local coastal region populations.
Correction: Santucci et al. Assessment of Posterior Dentoalveolar Expansion with Invisalign in Adult Patients. Int. J. Environ. Res. Public Health 2023, 20, 4318
Authors: Santucci Vincent , Rossouw Paul Emile , Michelogiannakis Dimitrios , El-Bialy Tarek , Feng Changyong
Published: July 2023

There was an error in the original publication [...]
Food Appreciation Scale Development and Dimensionality Assessment
Authors: Cosgrove Kelly , Wharton Christopher
Published: July 2023

Food appreciation has been associated with favorable dietary and food waste behaviors. However, no validated food appreciation assessment currently exists. This study aimed to develop and validate a food appreciation scale (FAS) using data from two independent US-based samples recruited online. The 29-item FAS was based on existing literature regarding appreciation as a psychological construct, mindful eating, and epicurean tendencies. In Study 1, 311 participants completed the FAS, and exploratory factor analysis (EFA) was conducted. In Study 2, 300 participants completed the FAS, and confirmatory factor analysis (CFA) was conducted to determine whether the factor structure remained consistent. The EFA indicated a good model fit for a four-factor structure after excluding six items that loaded on multiple or no factors (TLI 0.95, CFI 0.97, SRMR 0.03, RMSEA 0.05), and Cronbach’s alpha indicated excellent reliability (Cronbach’s alpha values 0.82–0.9). The CFA confirmed the four-factor structure (TLI 0.97, CFI 0.98, SRMR 0.08, RMSEA 0.05) and acceptable factor loadings with a simple structure. The factors assessed active food appreciation, reflective food appreciation, mindful epicurean tendencies, and food-related rituals. The validated FAS could allow researchers to assess food appreciation, measure changes in food appreciation over time, and compare food appreciation among different study populations.
The Market’s View on Accounting Classifications for Asset Securitizations
Authors: Ahn Minkwan
Published: July 2023

Prior research has examined how investors view asset securitizations, and shows that investors treat securitizations as borrowings, even when GAAP treats them as sales. Upon the adoption of two new accounting standards relating to securitizations, some off-balance-sheet securitized assets were consolidated back onto firms’ balance sheets. This study investigated how investors viewed assets that firms consolidated under the new standards and those that firms left unconsolidated. I found that investors differentiated between these two types of securitizations, treating the consolidated assets as borrowings and the unconsolidated assets as sales. I conclude that the new accounting standards are more consistent with equity investors’ views of securitizations. I also found that, for the consolidated assets, investors did not distinguish between securitizations going through two different accounting structures. Lastly, this study provides evidence on one information channel that investors use to distinguish between securitizations that may have the economic substance of borrowings versus sales.
GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury
Authors: Xie Ming , Xie Ruiyan , Huang Pengcheng , Yap Desmond Y. H. , Wu Peng
Published: July 2023

Chromatin regulators (CRs) are essential upstream regulatory factors of epigenetic modification. The role of CRs in the pathogenesis of renal ischemia-reperfusion injury (IRI) remains unclear. We analyzed a bioinformatic analysis on the differentially expressed chromatin regulator genes in renal IRI patients using data from public domains. The hub CRs identified were used to develop a risk prediction model for renal IRI, and their expressions were also validated using Western blot, qRT-PCR, and immunohistochemistry in a murine renal IRI model. We also examined the relationships between hub CRs and infiltrating immune cells in renal IRI and used network analysis to explore drugs that target hub CRs and their relevant downstream microRNAs. The results of machine learning methods showed that five genes (DUSP1, GADD45A, GADD45B, GADD45G, HSPA1A) were upregulated in renal IRI, with key roles in the cell cycle, p38 MAPK signaling pathway, p53 signaling pathway, FoxO signaling pathway, and NF-κB signaling pathway. Two genes from the network, GADD45A and GADD45B (growth arrest and DNA damage-inducible protein 45 alpha and beta), were chosen for the renal IRI risk prediction model. They all showed good performance in the testing and validation cohorts. Mice with renal IRI showed significantly upregulated GADD45A and GADD45B expression within kidneys compared to sham-operated mice. GADD45A and GADD45B showed correlations with plasmacytoid dendritic cells (pDCs) in infiltrating immune cell analysis and enrichment in the MAPK pathway based on the weighted gene co-expression network analysis (WGCNA) method. Candidate drugs that target GADD45A and GADD45B include beta-escin, sertraline, primaquine, pimozide, and azacyclonol. The dysregulation of GADD45A and GADD45B is related to renal IRI and the infiltration of pDCs, and drugs that target GADD45A and GADD45B may have therapeutic potential for renal IRI.
APOA2: New Target for Molecular Hydrogen Therapy in Sepsis-Related Lung Injury Based on Proteomic and Genomic Analysis
Authors: Wang Yuanlin , Fan Yan , Jiang Yi , Wang Enquan , Song Yu , Chen Hongguang , Xu Feier , Xie Keliang , Yu Yonghao
Published: July 2023

Target biomarkers for H2 at both the protein and genome levels are still unclear. In this study, quantitative proteomics acquired from a mouse model were first analyzed. At the same time, functional pathway analysis helped identify functional pathways at the protein level. Then, bioinformatics on mRNA sequencing data were conducted between sepsis and normal mouse models. Differential expressional genes with the closest relationship to disease status and development were identified through module correlation analysis. Then, common biomarkers in proteomics and transcriptomics were extracted as target biomarkers. Through analyzing expression quantitative trait locus (eQTL) and genome-wide association studies (GWAS), colocalization analysis on Apoa2 and sepsis phenotype was conducted by summary-data-based Mendelian randomization (SMR). Then, two-sample and drug-target, syndrome Mendelian randomization (MR) analyses were all conducted using the Twosample R package. For protein level, protein quantitative trait loci (pQTLs) of the target biomarker were also included in MR. Animal experiments helped validate these results. As a result, Apoa2 protein or mRNA was identified as a target biomarker for H2 with a protective, causal relationship with sepsis. HDL and type 2 diabetes were proven to possess causal relationships with sepsis. The agitation and inhibition of Apoa2 were indicated to influence sepsis and related syndromes. In conclusion, we first proposed Apoa2 as a target for H2 treatment.
Screening for Novel Inhibitors of Amyloid Beta Aggregation and Toxicity as Potential Drugs for Alzheimer’s Disease
Authors: Sharari Sanaa , Vaikath Nishant N. , Tsakou Magdalini , Ghanem Simona S. , Vekrellis Kostas
Published: July 2023

AD is the most common neurodegenerative disorder characterized by progressive memory impairment and cognitive deficits. The pathology of AD is still unclear; however, several studies have shown that the aggregation of the Aβ peptide in the CNS is an exclusively pathological process involved in AD. Currently, there is no proven medication to cure or prevent the disease progression. Nevertheless, various therapeutic approaches for AD show only relief of symptoms and mostly work on cognitive recovery. However, one of the promising approaches for therapeutic intervention is to use inhibitors for blocking the Aβ peptide aggregation process. Recently, herbal phenolic compounds have been shown to have a therapeutic property for treatment of AD due to their multifaceted action. In this study, we investigated the effectiveness of SA, Gn Rb1, and DMyr on inhibiting the aggregation and toxicity of Aβ40 and Aβ42 using different biochemical and cell-based assays. Our results showed that SA and DMyr inhibit Aβ40 and Aβ42 fibrillation, seeded aggregation, and toxicity. Gn Rb1 did not have any effect on the aggregation or toxicity induced by Aβ40 and Aβ42. Moreover, SA and DMyr were able to disaggregate the preformed fibrils. Overall, these compounds may be used alone or synergistically and could be considered as a lead for designing new compounds that could be used as effective treatment of AD and related disorders.
Low Glucose plus β-Hydroxybutyrate Induces an Enhanced Inflammatory Response in Yak Alveolar Macrophages via Activating the GPR109A/NF-κB Signaling Pathway
Authors: Qi Jiancheng , Yang Qiyuan , Xia Qing , Huang Fangyuan , Guo Hongrui , Cui Hengmin , Xie Yue , Ren Zhihua , Gou Liping , Cai Dongjie , Kumbhar Maqsood Ahmed , Fang Jing , Zuo Zhicai
Published: July 2023

Yaks are often subject to long-term starvation and a high prevalence of respiratory diseases and mortality in the withered season, yet the mechanisms that cause this remain unclear. Research has demonstrated that β-hydroxybutyrate (BHB) plays a significant role in regulating the immune system. Hence, we hypothesize that the low glucose and high BHB condition induced by severe starvation might have an effect on the pro-inflammatory response of the alveolar macrophages (AMs) in yaks. To validate our hypothesis, we isolated and identified primary AMs from freshly slaughtered yaks and cultured them in a medium with 5.5 mM of glucose or 2.8 mM of glucose plus 1–4 mM of BHB. Utilizing a real-time quantitative polymerase chain reaction (RT-qPCR), immunoblot assay, and enzyme-linked immunosorbent assay (ELISA), we evaluated the gene and protein expression levels of GPR109A (G-protein-coupled receptor 109A), NF-κB p65, p38, and PPARγ and the concentrations of pro-inflammatory cytokines interleukin (IL)-1β and IL-6 and tumor necrosis factor (TNF)-α in the supernatant. The results demonstrated that AMs exposed to low glucose plus BHB had significantly higher levels of IL-1β, IL-6, and TNF-α (p < 0.05) and higher activity of the GPR109A/NF-κB signaling pathway. A pretreatment of either pertussis toxin (PTX, inhibitor of GPR109A) or pyrrolidinedithiocarbamic (PDTC, inhibitor of NF-κB p65) was effective in preventing the elevated secretion of pro-inflammatory cytokines induced by low glucose plus BHB (p < 0.05). These results indicated that the low glucose plus BHB condition would induce an enhanced pro-inflammatory response through the activation of the GPR109A/NF-κB signaling pathway in primary yak AMs, which is probably the reason why yaks experience a higher rate of respiratory diseases and mortality. This study will offer new insight into the prevention and treatment of bovine respiratory diseases.
SF3B1 Mutations Are Associated with Resistance to Non-Genotoxic MDM2 Inhibition in Chronic Lymphocytic Leukemia
Authors: Aptullahoglu Erhan , Wallis Jonathan P. , Marr Helen , Marshall Scott , Bown Nick , Willmore Elaine , Lunec John
Published: July 2023

Chronic lymphocytic leukemia (CLL) is a genetically and clinically heterogeneous malignancy affecting older individuals. There are a number of current treatment options for CLL, including monoclonal antibodies, targeted drugs, chemotherapy, and different combinations of these. However, for those patients who are intrinsically treatment resistant, or relapse following initial responses, novel targeted therapies are still needed. Targeting the mouse double-minute-2 human homolog (MDM2), a primary negative regulator of p53, is an appealing therapeutic strategy for non-genotoxic reactivation of p53, since the TP53 gene is in its wild-type state at diagnosis in approximately 90% of patients. Mutated SF3B1 and TP53 are both associated with more aggressive disease, resistance to therapies and poorer overall survival for CLL. In this study, we performed a screen for SF3B1 and TP53 mutations and tested RG7388 (idasanutlin), a second-generation MDM2 inhibitor, in a cohort of CLL primary patient samples. SF3B1 mutations were detected in 24 of 195 cases (12.3%) and found associated with poor overall survival (hazard ratio [HR] 2.12, p = 0.032) and high CD38 expression (median CD38 (%) 32 vs. 5; p = 0.0087). The novel striking finding of this study was an independent link between SF3B1 mutational status and poor response to RG7388. Overall, SF3B1 mutations in CLL patient samples were associated with resistance to treatment with RG7388 ex vivo, and patients with the wild type for both SF3B1 and TP53 are more likely to benefit from treatment with MDM2 inhibitors.
Is It Possible to Obtain a Product of the Desired Configuration from a Single Knoevenagel Condensation? Isomerization vs. Stereodefined Synthesis
Authors: Novikova Daria , Grigoreva Tatyana , Gurzhiy Vladislav , Tribulovich Vyacheslav
Published: July 2023

The biological activity of compounds directly depends on the three-dimensional arrangement of affinity fragments since a high degree of pharmacophore compliance with the binding site is required. 3-Benzylidene oxindoles are privileged structures due to their wide spectrum of biological activity, synthetic availability, and ease of modification. In particular, both kinase inhibitors and kinase activators can be found among 3-benzylidene oxindoles. In this work, we studied model compounds obtained via oxindole condensation with aldehydes and alkylphenones. These condensation products can exist in the form of E- and Z-isomers and also undergo isomerization in solutions. The factors causing isomeric transformation of these compounds were established. Comparative kinetic studies to obtain quantitative characteristics of UV-driven isomerization were first performed. The results obtained indicate dramatic differences in two subclasses, which should be considered when developing biologically active molecules.