bluuebunny/crossref_metadata_2025 huggingface dataset:
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in Bombyx mori
Authors: Li Gang , Zhang Xiao , Qian Heying , Liu Mingzhu , Zhao Guodong , Xu Anying
Published: December 2019

Fluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occur at the metabolic level of silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) to analyze the changes in midgut tissue after NaF stress using silkworm strains 733xin (susceptible stain) and T6 (strain resistant to fluoride), which were previously reported by our laboratory. Differential metabolomics analysis showed that both T6 and 733xin strains displayed complex responses after exposure to 200 mg/kg NaF. The purine metabolism and arginine and proline metabolic pathways of fluoride-tolerant strains reached significant levels, among which 3′-adenylic acid and hypoxanthine were significantly upregulated, whereas guanine, allantoic acid, xanthine, N-acetyl-L-glutamic acid, and pyruvate were significantly downregulated. These metabolic pathways may be related to the fluoride tolerance mechanism of NaF poisoning and tolerant strains.
Changes in Energy Reserves and Gene Expression Elicited by Freezing and Supercooling in the Antarctic Midge, Belgica antarctica
Authors: Teets Nicholas M. , Dalrymple Emma G. , Hillis Maya H. , Gantz J. D. , Spacht Drew E. , Lee Richard E. , Denlinger David L.
Published: December 2019

Freeze-tolerance, or the ability to survive internal ice formation, is relatively rare among insects. Larvae of the Antarctic midge Belgica antarctica are freeze-tolerant year-round, but in dry environments, the larvae can remain supercooled (i.e., unfrozen) at subzero temperatures. In previous work with summer-acclimatized larvae, we showed that freezing is considerably more stressful than remaining supercooled. Here, these findings are extended by comparing survival, tissue damage, energetic costs, and stress gene expression in larvae that have undergone an artificial winter acclimation regime and are either frozen or supercooled at −5 °C. In contrast to summer larvae, winter larvae survive at −5 °C equally well for up to 14 days, whether frozen or supercooled, and there is no tissue damage at these conditions. In subsequent experiments, we measured energy stores and stress gene expression following cold exposure at −5 °C for either 24 h or 14 days, with and without a 12 h recovery period. We observed slight energetic costs to freezing, as frozen larvae tended to have lower glycogen stores across all groups. In addition, the abundance of two heat shock protein transcripts, hsp60 and hsp90, tended to be higher in frozen larvae, indicating higher levels of protein damage following freezing. Together, these results indicate a slight cost to being frozen relative to remaining supercooled, which may have implications for the selection of hibernacula and responses to climate change.
Immunological and Clinical Impact of Manipulated and Unmanipulated DLI after Allogeneic Stem Cell Transplantation of AML Patients
Authors: Greiner Jochen , Götz Marlies , Bunjes Donald , Hofmann Susanne , Wais Verena
Published: December 2019

Allogeneic stem cell transplantation (allo-SCT) is the preferred curative treatment for several hematological malignancies. The efficacy of allo-SCT depends on the graft-versus-leukemia (GvL) effect. However, the prognosis of patients with relapsed acute myeloid leukemia (AML) following allo-SCT is poor. Donor lymphocyte infusion (DLI) is utilized after allo-SCT in this setting to prevent relapse, to prolong progression free survival, to establish full donor chimerism and to restore the GvL effect in patients with hematological malignancies. Thus, there are different options for the administration of DLI in AML patients. DLI is currently used prophylactically and in the setting of an overt relapse. In addition, in the minimal residual disease (MRD) setting, DLI may be a possibility to improve overall survival. However, DLI might increase the risk of severe life-threatening complications such as graft-versus-host disease (GvHD) as well as severe infections. The transfusion of lymphocytes has been tested not only for the treatment of hematological malignancies but also chronic infections. In this context, manipulated DLI in a prophylactic or therapeutic approach are an option, e.g., virus-specific DLI using different selection methods or antigen-specific DLI such as peptide-specific CD8+ cytotoxic T lymphocytes (CTLs). In addition, T cells are also genetically engineered, using both chimeric antigen receptor (CAR) genetically modified T cells and T cell receptor (TCR) genetically modified T cells. T cell therapies in general have the potential to enhance antitumor immunity, augment vaccine efficacy, and limit graft-versus-host disease after allo-SCT. The focus of this review is to discuss the different strategies to use donor lymphocytes after allo-SCT. Our objective is to give an insight into the functional effects of DLI on immunogenic antigen recognition for a better understanding of the mechanisms of DLI. To ultimately increase the GvL potency without raising the risk of GvHD at the same time.
An Educational Study Promoting the Delivery of Transcranial Doppler Ultrasound Screening in Paediatric Sickle Cell Disease: A European Multi-Centre Perspective
Authors: Inusa Baba P. D. , Sainati Laura , MacMahon Corrina , Colombatti Raffaella , Casale Maddalena , Perrotta Silverio , Rampazzo Paola , Hemmaway Claire , Padayachee Soundrie T.
Published: December 2019

Background: Effective stroke prevention in sickle cell disease (SCD) is recommended for children with sickle cell anaemia. Effective implementation relies on the correct stratification of stroke risk using Transcranial Doppler Ultrasound (TCD), prior to committing children to long-term treatment with transfusion. Nevertheless, less than 50% of children with SCD in Europe receive annual TCD—one of the reasons being a lack of trained personnel. The present European multi-centre study was designed to promote the standardisation and delivery of effective screening. Methods: Fifty-five practitioners from differing professional backgrounds were recruited to the TCD training program. The impact of the training programme was evaluated in three European haematology clinics by comparing stroke risk classification and middle cerebral artery time-averaged maximum velocity (TAMMV) obtained from a cohort of 555 patients, before and after training. Results: 42% (23/55) of trainees successfully completed the program. The TAMMV, used to predict stroke risk at each Centre, demonstrated the highest values in Centre 3 (p < 0.0001) before training. The imaging-TCD TAMMV was also higher in Centre 3 (p < 0.001). Following training, the TAMMV showed closer agreement between centres for both imaging-TCD and non-imaging TCD. The stroke risk distribution of children at each centre varied significantly before training (p < 0.001), but improved after training (Fisher’s Exact: no treatment = 5.6, p = 0.41, treatment = 13.8, p < 0.01). The same consistency in stroke risk distribution following training was demonstrated with both non-imaging and imaging-TCD data. Conclusion: The attainment of competency in stroke screening using transcranial Doppler scanning (TCD) in sickle cell disease is more feasible for professionals with an ultrasound imaging background. A quality assurance (QA) system is required to ensure that standards are maintained. Further work is in progress to develop an achievable and reproducible QA program.
Favorable 90-Day Mortality in Obese Caucasian Patients with Septic Shock According to the Sepsis-3 Definition
Authors: Mewes Caspar , Böhnke Carolin , Alexander Tessa , Büttner Benedikt , Hinz José , Popov Aron-Frederik , Ghadimi Michael , Beißbarth Tim , Raddatz Dirk , Meissner Konrad , Quintel Michael , Bergmann Ingo , Mansur Ashham
Published: December 2019

Septic shock is a frequent life-threatening condition and a leading cause of mortality in intensive care units (ICUs). Previous investigations have reported a potentially protective effect of obesity in septic shock patients. However, prior results have been inconsistent, focused on short-term in-hospital mortality and inadequately adjusted for confounders, and they have rarely applied the currently valid Sepsis-3 definition criteria for septic shock. This investigation examined the effect of obesity on 90-day mortality in patients with septic shock selected from a prospectively enrolled cohort of septic patients. A total of 352 patients who met the Sepsis-3 criteria for septic shock were enrolled in this study. Body-mass index (BMI) was used to divide the cohort into 24% obese (BMI ≥ 30 kg/m2) and 76% non-obese (BMI < 30 kg/m2) patients. Kaplan-Meier survival analysis revealed a significantly lower 90-day mortality (31% vs. 43%; p = 0.0436) in obese patients compared to non-obese patients. Additional analyses of baseline characteristics, disease severity, and microbiological findings outlined further statistically significant differences among the groups. Multivariate Cox regression analysis estimated a significant protective effect of obesity on 90-day mortality after adjustment for confounders. An understanding of the underlying physiologic mechanisms may improve therapeutic strategies and patient prognosis.
Energy-Efficient Architecture for CNNs Inference on Heterogeneous FPGA
Authors: Spagnolo Fanny , Perri Stefania , Frustaci Fabio , Corsonello Pasquale
Published: December 2019

Due to the huge requirements in terms of both computational and memory capabilities, implementing energy-efficient and high-performance Convolutional Neural Networks (CNNs) by exploiting embedded systems still represents a major challenge for hardware designers. This paper presents the complete design of a heterogeneous embedded system realized by using a Field-Programmable Gate Array Systems-on-Chip (SoC) and suitable to accelerate the inference of Convolutional Neural Networks in power-constrained environments, such as those related to IoT applications. The proposed architecture is validated through its exploitation in large-scale CNNs on low-cost devices. The prototype realized on a Zynq XC7Z045 device achieves a power efficiency up to 135 Gops/W. When the VGG-16 model is inferred, a frame rate up to 11.8 fps is reached.
Shoreline Extraction Based on an Active Connection Matrix (ACM) Image Enhancement Strategy
Authors: Zollini Sara , Alicandro Maria , Cuevas-González María , Baiocchi Valerio , Dominici Donatella , Buscema Paolo Massimo
Published: December 2019

Coastal environments are facing constant changes over time due to their dynamic nature and geological, geomorphological, hydrodynamic, biological, climatic and anthropogenic factors. For these reasons, the monitoring of these areas is crucial for the safeguarding of the cultural heritage and the populations living there. The focus of this paper is shoreline extraction by means of an experimental algorithm, called J-Net Dynamic (Semeion Research Center of Sciences of Communication, Rome, Italy). It was tested on two types of image: a very high resolution (VHR) multispectral image (WorldView-2) and a high resolution (HR) radar synthetic aperture radar (SAR) image (Sentinel-1). The extracted shorelines were compared with those manually digitized for both images independently. The results obtained with the J-Net Dynamic algorithm were also compared with common algorithms, widely used in the literature, including the WorldView water index and the Canny edge detector. The results show that the experimental algorithm is more effective than the others, as it improves shoreline extraction accuracy both in the optical and SAR images.
Chromosome-Level Comprehensive Genome of Mangrove Sediment-Derived Fungus Penicillium variabile HXQ-H-1
Authors: Peng Ling , Li Liangwei , Liu Xiaochuan , Chen Jianwei , Shi Chengcheng , Guo Wenjie , Xu Qiwu , Fan Guangyi , Liu Xin , Li Dehai
Published: December 2019

Penicillium is an ascomycetous genus widely distributed in the natural environment and is one of the dominant fungi involved in the decomposition of mangroves, which can produce a variety of antitumor compounds and bioactive substances. However, in mangrove ecosystems there is no complete genome in this genus. In this study, we isolated a fungus strain named Penicillium variabile HXQ-H-1 from coast mangrove (Fujian Province, China). We generated a chromosome-level genome with total size of 33.32 Mb, scaffold N50 of 5.23 Mb and contig N50 of 96.74 kb. Additionally, we anchored about 95.91% assembly sequences into the longest seven scaffolds, and predicted 10,622 protein-coding genes, in which 99.66% could be annotated by eight protein databases. The secondary metabolites analysis reveals the strain has various gene clusters involving polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and terpene synthase that may have a largely capacity of biotechnological potential. Comparison genome analysis between Penicillium variabile and Talaromyces islandicus reveals a small difference in the total number of genes, whereas HXQ-H-1 has a higher gene number with COG functional annotation. Evolutionary relationship of Penicillum based on genome-wide data was carried out for the first time, showing the strain HXQ-H-1 is closely related to Talaromyces islandicus. This genomic resource may provide a new resource for development of novel bioactive antibiotics, drug candidates and precursors in Penicillium variabile.
N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi
Authors: Min Kyunghun , Naseem Shamoon , Konopka James B.
Published: December 2019

N-acetylglucosamine (GlcNAc) is being increasingly recognized for its ability to stimulate cell signaling. This amino sugar is best known as a component of cell wall peptidoglycan in bacteria, cell wall chitin in fungi and parasites, exoskeletons of arthropods, and the extracellular matrix of animal cells. In addition to these structural roles, GlcNAc is now known to stimulate morphological and stress responses in a wide range of organisms. In fungi, the model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe lack the ability to respond to GlcNAc or catabolize it, so studies with the human pathogen Candida albicans have been providing new insights into the ability of GlcNAc to stimulate cellular responses. GlcNAc potently induces C. albicans to transition from budding to filamentous hyphal growth. It also promotes an epigenetic switch from White to Opaque cells, which differ in morphology, metabolism, and virulence properties. These studies have led to new discoveries, such as the identification of the first eukaryotic GlcNAc transporter. Other results have shown that GlcNAc can induce signaling in C. albicans in two ways. One is to act as a signaling molecule independent of its catabolism, and the other is that its catabolism can cause the alkalinization of the extracellular environment, which provides an additional stimulus to form hyphae. GlcNAc also induces the expression of virulence genes in the C. albicans, indicating it can influence pathogenesis. Therefore, this review will describe the recent advances in understanding the role of GlcNAc signaling pathways in regulating C. albicans morphogenesis and virulence.
Bicyclo[4.2.0]octa-1,3,5-trien-3-yl-dimethyl((E)-styryl)-silane
Authors: Levchenko Konstantin S. , Chudov Konstantin A. , Demin Dmitri Yu. , Shmelin Pavel S. , Grebennikov Evgeny P.
Published: December 2019

Bicyclo[4.2.0]octa-1,3,5-trien-3-yl-dimethyl-((E)-styryl)-silane was synthesized via three stage synthesis starting from benzocyclobutene and (2-bromo-vinyl)-benzene. The structure of the product was determined using 1H- and 13C-NMR and HRMS.
Modifications of Dental Implant Surfaces at the Micro- and Nano-Level for Enhanced Osseointegration
Authors: Yeo In-Sung Luke
Published: December 2019

This review paper describes several recent modification methods for biocompatible titanium dental implant surfaces. The micro-roughened surfaces reviewed in the literature are sandblasted, large-grit, acid-etched, and anodically oxidized. These globally-used surfaces have been clinically investigated, showing survival rates higher than 95%. In the past, dental clinicians believed that eukaryotic cells for osteogenesis did not recognize the changes of the nanostructures of dental implant surfaces. However, research findings have recently shown that osteogenic cells respond to chemical and morphological changes at a nanoscale on the surfaces, including titanium dioxide nanotube arrangements, functional peptide coatings, fluoride treatments, calcium–phosphorus applications, and ultraviolet photofunctionalization. Some of the nano-level modifications have not yet been clinically evaluated. However, these modified dental implant surfaces at the nanoscale have shown excellent in vitro and in vivo results, and thus promising potential future clinical use.
Releasing Behavior of Lipopolysaccharide from Gelatin Modulates Inflammation, Cellular Senescence, and Bone Formation in Critical-Sized Bone Defects in Rat Calvaria
Authors: Zhao Jianxin , Honda Yoshitomo , Tanaka Tomonari , Hashimoto Yoshiya , Matsumoto Naoyuki
Published: December 2019

Lipopolysaccharide (LPS) is a well-known strong inducer of inflammation. However, there is little information regarding how LPS-release behavior affects cellular senescence at the affected area. In this paper, we demonstrate that a vacuum-heating technique (dehydrothermal treatment) can be utilized to prepare an LPS sustained-release gelatin sponge (LS-G). LPS sustained release from gelatin leads to the long-term existence of senescent cells in critical-sized bone defects in rat calvaria. Three types of gelatin sponges were prepared in this study: a medical-grade gelatin sponge with extremely low LPS levels (MG), LS-G, and a LPS rapid-release gelatin sponge (LR-G). Histological (H-E) and immunohistochemical (COX-2, p16, and p21) staining were utilized to evaluate inflammatory reactions and cellular senescence one to three weeks after surgery. Soft X-ray imaging was utilized to estimate new bone formation in the defects. The LR-G led to stronger swelling and COX-2 expression in defects compared to the MG and LS-G at 1 week. Despite a small inflammatory reaction, LS-G implantation led to the long-term existence of senescent cells and hampered bone formation compared to the MG and LR-G. These results suggest that vacuum heating is a viable technique for preparing different types of materials for releasing bacterial components, which is helpful for developing disease models for elucidating cellular senescence and bone regeneration.
The Effect of Layer Thicknesses in Hybrid Titanium–Carbon Laminates on Low-Velocity Impact Response
Authors: Jakubczak Patryk , Bieniaś Jarosław , Droździel Magda , Podolak Piotr , Harmasz Aleksandra
Published: December 2019

The purpose of the work was the effect of metal volume fraction of fiber metal laminates on damage after dynamic loads based upon the example of innovative hybrid titanium–carbon composite laminates. The subject of the study was metal–fiber hybrid titanium–carbon composite laminates. Four types of hybrid titanium–carbon laminates were designed with various metal volume fraction coefficient but constant thickness. Based on the results, it can be stated that changes in the metal volume fraction coefficient in the range of 0.375–0.6 in constant thickness titanium–carbon composite laminates do not significantly affect their resistance to impacts in the energy range of 5–45 J. It was concluded that there were no significant differences in maximum force values, total contact time, and damage range. Some tendency towards a reduction in the energy accumulation capacity was observed with an increase in thickness of the metal part in relation to the total thickness of the laminate, especially in the lower impact energy range. This can result in the lower bending stiffness of laminates with lower metal content and potential elastic strain of the composite part before the initiation of the fiber damage process.
Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin
Authors: Nguyen Van Bon , Chen Shan-Ping , Nguyen Thi Hanh , Nguyen Minh Trung , Tran Thi Thanh Thao , Doan Chien Thang , Tran Thi Ngoc , Nguyen Anh Dzung , Kuo Yao-Haur , Wang San-Lang
Published: December 2019

Marine chitins (MC) have been utilized for the production of vast array of bioactive products, including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds. The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG), via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011 with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of 5.65–6.15 containing 1% α-chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was kept at 25 °C for 2 d. Notably, α-chitin was newly investigated as the major potential material for PG production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06, 0.04, 0.04, and 0.2 µg/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold efficacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest that marine chitins are valuable sources for production of prodigiosin, a potential candidate for cancer drugs.
Effect of Carrageenans on Vegetable Jelly in Humans with Hypercholesterolemia
Authors: Valado Ana , Pereira Maria , Caseiro Armando , Figueiredo João P. , Loureiro Helena , Almeida Carla , Cotas João , Pereira Leonel
Published: December 2019

Changes in lipid profile constitute the main risk factor for cardiovascular diseases. Algae extracted carrageenans are long-chain polysaccharides and their ability to form gels provides for the formation of vegetable jelly. The objective was to evaluate the bioactive potential of carrageenan (E407) in the lipid profile, after ingestion of jelly. A total of 30 volunteers of both sexes, aged 20–64 years and with total cholesterol (TC) values ≥200 mg/dL, who ingested 100 mL/day of jelly for 60 days, were studied. All had two venous blood collections: before starting the jelly intake and after 60 days. At both times, TC, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG), were evaluated using commercial kits and spectrophotometer. The statistics were performed using the SPSS 25.0 software and p < 0.05 were considered statistically significant. Serum values after 60 days of jelly intake revealed a statistically significant decrease in TC levels (5.3%; p = 0.001) and LDL-C concentration (5.4%; p = 0.048) in females. The daily intake of vegetable jelly for 60 days showed a reduction in serum TC and LDL-C levels in women, allowing us to conclude that carrageenan has bioactive potential in reducing TC concentration.
Affinity Membranes and Monoliths for Protein Purification
Authors: Lalli Eleonora , Silva Jouciane S. , Boi Cristiana , Sarti Giulio C.
Published: December 2019

Affinity capture represents an important step in downstream processing of proteins and it is conventionally performed through a chromatographic process. The performance of this step highly depends on the type of matrix employed. In particular, resin beads and convective materials, such as membranes and monoliths, are the commonly available supports. The present work deals with non-competitive binding of bovine serum albumin (BSA) on different chromatographic media functionalized with Cibacron Blue F3GA (CB). The aim is to set up the development of the purification process starting from the lab-scale characterization of a commercially available CB resin, regenerated cellulose membranes and polymeric monoliths, functionalized with CB to identify the best option. The performance of the three different chromatographic media is evaluated in terms of BSA binding capacity and productivity. The experimental investigation shows promising results for regenerated cellulose membranes and monoliths, whose performance are comparable with those of the packed column tested. It was demonstrated that the capacity of convective stationary phases does not depend on flow rate, in the range investigated, and that the productivity that can be achieved with membranes is 10 to 20 times higher depending on the initial BSA concentration value, and with monoliths it is approximately twice that of beads, at the same superficial velocity.
Heat-Affected-Zone Liquation Cracking in Welded Cast Haynes® 282®
Authors: Singh Sukhdeep , Andersson Joel
Published: December 2019

Varestraint weldability testing and Gleeble thermomechanical simulation of the newly developed cast form of Haynes® 282® were performed to understand how heat-affected-zone (HAZ) liquation cracking is influenced by different preweld heat treatments. In contrast to common understanding, cracking susceptibility did not improve with a higher degree of homogenization achieved at a higher heat-treatment temperature. Heat treatments with a 4 h dwell time at 1120 °C and 1160 °C exhibited low cracking sensitivity, whereas by increasing the temperature to 1190 °C, the cracking was exacerbated. Nanosecond ion mass spectrometry analysis was done to characterize B segregation at grain boundaries that the 1190 °C heat treatment indicated to be liberated from the dissolution of C–B rich precipitates.
A Comparative Study on the Influence of Chromium on the Phase Fraction and Elemental Distribution in As-Cast High Chromium Cast Irons: Simulation vs. Experimentation
Authors: Nayak U. Pranav , Guitar María Agustina , Mücklich Frank
Published: December 2019

The excellent abrasion resistance of high chromium cast irons (HCCIs) stems from the dispersion of the hard iron-chromium eutectic carbides. The surrounding matrix on the other hand, provides sufficient mechanical support, improving the resistance to cracking deformation and spalling. Prior knowledge of the microstructural characteristics is imperative to appropriately design subsequent heat treatments, and in this regard, employing computational tools is the current trend. In this work, computational and experimental results were correlated with the aim of validating the usage of MatCalc simulations to predict the eutectic carbide phase fraction and the elemental distribution in two HCCI alloys, in the as-cast condition. Microstructural observations were carried out using optical microscopy and SEM. The chemical composition and fraction of each phase was measured by electron probe microanalysis and image analysis, respectively. In all cases, the values predicted by the pseudo-equilibrium diagrams, computed with MatCalc, were in accordance with the experimentally determined values. Consequently, the results suggest that time and resource intensive experimental procedures can be replaced by simulation techniques to determine the phase fraction and especially, the individual phase compositions in the as-cast state.
Reductive Smelting of Neutralized Red Mud for Iron Recovery and Produced Pig Iron for Heat-Resistant Castings
Authors: Valeev Dmitry , Zinoveev Dmitry , Kondratiev Alex , Lubyanoi Dmitry , Pankratov Denis
Published: December 2019

The chemical and mineral composition of the red mud from the Ural Aluminum Plant were studied by XRF, XRD, and Mössbauer spectroscopy. Experiments on reductive smelting of red mud were carried out in a range of temperatures (1650–1750 °C) to recover iron from the aluminum production waste with maximum efficiency. It was found that it is possible to obtain pig iron with a high content of titanium, phosphorus, and vanadium, and low sulfur content. The efficiency of iron recovery at 1750 °C was found to be around 98%. Thermodynamic calculations were carried out to assist in finding the optimal conditions for the process (e.g., carbon content, furnace temperature, slag liquidus temperature). It was also found that the pig iron phase obtained at 1650 to 1700 °C is not separated from the slag phase into ingot compared with the sample obtained at 1750 °C. Pig iron obtained at 1750 °C can be used to produce molds for the steel-casting equipment.
Establish Using FEM Method of Constitutive Model for Chip Formation in the Cutting Process of Gray Cast Iron
Authors: Tu Lihui , Shi Weimin
Published: December 2019

The constitutive equation of mechanics, namely the stress–strain model of the material, is used to describe the mathematical expression of the mechanical properties of the material (stress–strain–intensity–time relationship). In the cutting simulation of metals, the material constitutive model needs to be established. To study the cutting mechanism of gray cast iron using the finite element method (FEM), a series of split Hopkinson press bar (SHPB) tests are carried out to achieve the stress–strain curves of gray cast iron under different strain rates and temperatures. In the cutting simulation of gray cast iron, the Johnson–Cook (JC) constitutive equation is used to reflect the mechanical behavior during the cutting process. The calculation of JC constants is analyzed deeply and obtained from the SHPB test data. One orthogonal finite element model is developed with the JC material model to reveal the cutting mechanism of gray cast iron. The chip formation, stress distribution, temperature distribution, and cutting force are investigated through the cutting simulation. At the same time, cutting experiments of gray cast iron are carried out for validating the simulation results. The results of the cutting simulation and experiments show a good agreement.
Novel α + β Type Ti-Fe-Cu Alloys Containing Sn with Pertinent Mechanical Properties
Authors: Zadorozhnyy Vladislav , Ketov Sergey V. , Wada Takeshi , Wurster Stefan , Nayak Vignesh , Louzguine-Luzgin Dmitri V. , Eckert Jürgen , Kato Hidemi
Published: December 2019

Rising demand for bone implants has led to the focus on future alternatives of alloys with better biocompatibility and mechanical strength. Thus, this research is dedicated to the synthesis and investigation of new compositions for low-alloyed Ti-based compounds, which conjoin relatively acceptable mechanical properties and low elastic moduli. In this regard, the structural and mechanical properties of α + β Ti-Fe-Cu-Sn alloys are described in the present paper. The alloys were fabricated by arc-melting and tilt-casting techniques which followed subsequent thermo-mechanical treatment aided by dual-axial forging and rolling procedures. The effect of the concentrations of the alloying elements, and other parameters, such as regimes of rolling and dual-axial forging operation, on the microstructure and mechanical properties were thoroughly investigated. The Ti94Fe1Cu1Sn4 alloy with the most promising mechanical properties was subjected to thermo-mechanical treatment. After a single rolling procedure at 750 °C, the alloy exhibited tensile strength and tensile plasticity of 1300 MPa and 6%, respectively, with an elastic modulus of 70 GPa. Such good tensile mechanical properties are explained by the optimal volume fraction balance between α and β phases and the texture alignment obtained, providing superior alternatives in comparison to pure α- titanium alloys.
Using Statistical Modeling to Predict the Electrical Energy Consumption of an Electric Arc Furnace Producing Stainless Steel
Authors: Carlsson Leo S. , Samuelsson Peter B. , Jönsson Pär G.
Published: December 2019

The non-linearity of the Electric Arc Furnace (EAF) process and the correlative behavior between the process variables impose challenges that have to be considered if one aims to create a statistical model that is relevant and useful in practice. In this regard, both the statistical modeling framework and the statistical tools used in the modeling pipeline must be selected with the aim of handling these challenges. To achieve this, a non-linear statistical modeling framework known as Artificial Neural Networks (ANN) has been used to predict the Electrical Energy (EE) consumption of an EAF producing stainless steel. The statistical tools Feature Importance (FI), Distance Correlation (dCor) and Kolmogorov–Smirnov (KS) tests are applied to investigate the most influencing input variables as well as reasons behind model performance differences when predicting the EE consumption on future heats. The performance, measured as kWh per heat, of the best model was comparable to the performance of the best model reported in the literature while requiring substantially fewer input variables.
Vascularized Microfluidics and the Blood–Endothelium Interface
Authors: Hesh Christopher A. , Qiu Yongzhi , Lam Wilbur A.
Published: December 2019

The microvasculature is the primary conduit through which the human body transmits oxygen, nutrients, and other biological information to its peripheral tissues. It does this through bidirectional communication between the blood, consisting of plasma and non-adherent cells, and the microvascular endothelium. Current understanding of this blood–endothelium interface has been predominantly derived from a combination of reductionist two-dimensional in vitro models and biologically complex in vivo animal models, both of which recapitulate the human microvasculature to varying but limited degrees. In an effort to address these limitations, vascularized microfluidics have become a platform of increasing importance as a consequence of their ability to isolate biologically complex phenomena while also recapitulating biochemical and biophysical behaviors known to be important to the function of the blood–endothelium interface. In this review, we discuss the basic principles of vascularized microfluidic fabrication, the contribution this platform has made to our understanding of the blood–endothelium interface in both homeostasis and disease, the limitations and challenges of these vascularized microfluidics for studying this interface, and how these inform future directions.
A Micromachined Metal Oxide Composite Dual Gas Sensor System for Principal Component Analysis-Based Multi-Monitoring of Noxious Gas Mixtures
Authors: Yang In-Hwan , Jin Joon-Hyung , Min Nam Ki
Published: December 2019

Microelectronic gas-sensor devices were developed for the detection of carbon monoxide (CO), nitrogen dioxides (NO2), ammonia (NH3) and formaldehyde (HCHO), and their gas-sensing characteristics in six different binary gas systems were examined using pattern-recognition methods. Four nanosized gas-sensing materials for these target gases, i.e., Pd-SnO2 for CO, In2O3 for NOX, Ru-WO3 for NH3, and SnO2-ZnO for HCHO, were synthesized using a sol-gel method, and sensor devices were fabricated using a microsensor platform. Principal component analysis of the experimental data from the microelectromechanical systems gas-sensor arrays under exposure to single gases and their mixtures indicated that identification of each individual gas in the mixture was successful. Additionally, the gas-sensing behavior toward the mixed gas indicated that the traditional adsorption and desorption mechanism of the n-type metal oxide semiconductor (MOS) governs the sensing mechanism of the mixed gas systems.
Design of a Compliant Mechanism Based Four-Stage Amplification Piezoelectric-Driven Asymmetric Microgripper
Authors: Chen Xiaodong , Deng Zilong , Hu Siya , Gao Jinhai , Gao Xingjun
Published: December 2019

The existing symmetrical microgrippers have larger output displacements compared with the asymmetrical counterparts. However, the two jaws of a symmetrical microgripper are less unlikely to offer the same forces on the two sides of a grasped micro-object due to the manufacture and assembly errors. Therefore, this paper proposes a new asymmetric microgripper to obtain stable output force of the gripper. Compared with symmetrical microgrippers, asymmetrical microgrippers usually have smaller output displacements. In order to increase the output displacement, a compliant mechanism with four stage amplification is employed to design the asymmetric microgripper. Consequently, the proposed asymmetrical microgripper possesses the advantages of both the stable output force of the gripper and large displacement amplification. To begin with, the mechanical model of the microgripper is established in this paper. The relationship between the driving force and the output displacement of the microgripper is then derived, followed by the static characteristics’ analysis of the microgripper. Furthermore, finite element analysis (FEA) of the microgripper is also performed, and the mechanical structure of the microgripper is optimized based on the FEA simulations. Lastly, experimental tests are carried out, with a 5.28% difference from the FEA results and an 8.8% difference from the theoretical results. The results from theoretical calculation, FEA simulations, and experimental tests verify that the displacement amplification ratio and the maximum gripping displacement of the microgripper are up to 31.6 and 632 μm, respectively.
Effects of Channel Wall Twisting on the Mixing in a T-Shaped Micro-Channel
Authors: Kang Dong Jin
Published: December 2019

A new design scheme is proposed for twisting the walls of a microchannel, and its performance is demonstrated numerically. The numerical study was carried out for a T-shaped microchannel with twist angles in the range of 0 to 34π. The Reynolds number range was 0.15 to 6. The T-shaped microchannel consists of two inlet branches and an outlet branch. The mixing performance was analyzed in terms of the degree of mixing and relative mixing cost. All numerical results show that the twisting scheme is an effective way to enhance the mixing in a T-shaped microchannel. The mixing enhancement is realized by the swirling of two fluids in the cross section and is more prominent as the Reynolds number decreases. The twist angle was optimized to maximize the degree of mixing (DOM), which increases with the length of the outlet branch. The twist angle was also optimized in terms of the relative mixing cost (MC). The two optimum twisting angles are generally not coincident. The optimum twist angle shows a dependence on the length of the outlet branch but it is not affected much by the Reynolds number.
Spread of Carbapenem-Resistant Klebsiella pneumoniae in Hub and Spoke Connected Health-Care Networks: A Case Study from Italy
Authors: Barbadoro Pamela , Dichiara Arianna , Arsego Daniele , Ponzio Elisa , Savini Sandra , Manso Esther , D’Errico Marcello M.
Published: December 2019

The study describes the spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) in a regional healthcare network in Italy. The project included several stages: (1) Establishment of a laboratory-based regional surveillance network, including all the acute care hospitals of the Marches Region (n = 20). (2) Adoption of a shared protocol for the surveillance of Multi-Drug Resistant Organisms (MDROs). Only the first CRKP isolate for each patient has been included in the surveillance in each hospital. The anonymous tracking of patients, and their subsequent microbial records within the hospital network, allowed detection of networks of inter-hospital exchange of CRKP and its comparison with transfer of patients within the hospital network. Pulsed-Field Gel Electrophoresis (PFGE) analysis has been used to study selected isolates belonging to different hospitals. 371,037 admitted patients have been included in the surveillance system. CRKP has shown an overall incidence rate of 41.0 per 100,000 days of stay (95% confidence interval, CI 38.5–43.5/100,000 DOS), a CRKP incidence rate of isolation in blood of 2.46/100,000 days of stay (95% CI 1.89–3.17/100,000 days of stay (DOS) has been registered; significant variability has been registered in facilities providing different levels of care. The network of CRKP patients’ exchange was correlated to that of the healthcare organization, with some inequalities and the identification of bridges in CRKP transfers. More than 73% of isolates were closely related. Patients’ exchange was an important route of spread of antimicrobial resistance, highlighting the pivotal role played by the hub, and selected institution to be used in prioritizing infection control efforts.
Tomato RNA-seq Data Mining Reveals the Taxonomic and Functional Diversity of Root-Associated Microbiota
Authors: Chialva Matteo , Ghignone Stefano , Novero Mara , Hozzein Wael N. , Lanfranco Luisa , Bonfante Paola
Published: December 2019

Next-generation approaches have enabled researchers to deeply study the plant microbiota and to reveal how microbiota associated with plant roots has key effects on plant nutrition, disease resistance, and plant development. Although early “omics” experiments focused mainly on the species composition of microbial communities, new “meta-omics” approaches such as meta-transcriptomics provide hints about the functions of the microbes when interacting with their plant host. Here, we used an RNA-seq dataset previously generated for tomato (Solanum lycopersicum) plants growing on different native soils to test the hypothesis that host-targeted transcriptomics can detect the taxonomic and functional diversity of root microbiota. Even though the sequencing throughput for the microbial populations was limited, we were able to reconstruct the microbial communities and obtain an overview of their functional diversity. Comparisons of the host transcriptome and the meta-transcriptome suggested that the composition and the metabolic activities of the microbiota shape plant responses at the molecular level. Despite the limitations, mining available next-generation sequencing datasets can provide unexpected results and potential benefits for microbiota research.
Gene Cloning, Recombinant Expression, Characterization, and Molecular Modeling of the Glycolytic Enzyme Triosephosphate Isomerase from Fusarium oxysporum
Authors: Hernández-Ochoa Beatriz , Gómez-Manzo Saúl , Alcaraz-Carmona Erick , Serrano-Posada Hugo , Centeno-Leija Sara , Arreguin-Espinosa Roberto , Cuevas-Cruz Miguel , González-Valdez Abigail , Mendoza-Espinoza José Alberto , Acosta Ramos Marcelo , Cortés-Maldonado Leyda , Montiel-González Alba Mónica , Pérez de la Cruz Verónica , Rocha-Ramírez Luz María , Marcial-Quino Jaime , Sierra-Palacios Edgar
Published: December 2019

Triosephosphate isomerase (TPI) is a glycolysis enzyme, which catalyzes the reversible isomerization between dihydroxyactetone-3-phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP). In pathogenic organisms, TPI is essential to obtain the energy used to survive and infect. Fusarium oxisporum (Fox) is a fungus of biotechnological importance due to its pathogenicity in different organisms, that is why the relevance of also biochemically analyzing its TPI, being the first report of its kind in a Fusarium. Moreover, the kinetic characteristics or structural determinants related to its function remain unknown. Here, the Tpi gene from F. oxysporum was isolated, cloned, and overexpressed. The recombinant protein named FoxTPI was purified (97% purity) showing a molecular mass of 27 kDa, with optimal activity at pH 8.0 and and temperature of 37 °C. The values obtained for Km and Vmax using the substrate GAP were 0.47 ± 0.1 mM, and 5331 μmol min−1 mg−1, respectively. Furthemore, a protein structural modeling showed that FoxTPI has the classical topology of TPIs conserved in other organisms, including the catalytic residues conserved in the active site (Lys12, His94 and Glu164). Finally, when FoxTPI was analyzed with inhibitors, it was found that one of them inhibits its activity, which gives us the perspective of future studies and its potential use against this pathogen.
Petrogenesis and Tectonic Setting of Ore-Associated Intrusive Rocks in the Baiyinnuoer Zn–Pb Deposit, Southern Great Xing’an Range (NE China): Constraints from Zircon U–Pb Dating, Geochemistry, and Sr–Nd–Pb Isotopes
Authors: Zhao Qing , Xiao Rongge , Zhang Dehui , Wang Jianping , Zhang Yanfei , Li Panpan
Published: December 2019

The Baiyinnuoer skarn Zn–Pb deposit, located in the Southern Great Xing’an Range, Northeast China, is the largest Zn–Pb deposit of the northern China, with a total reserve of 32.74 Mt at average grades of 5.44% Zn and 2.02% Pb. The Zn–Pb ore bodies are hosted in the Lower Permian Huanggangliang Formation. The results of zircon U–Pb geochronology show that the ore-associated granodiorite porphyry, granodiorite, and diorite were emplaced at 248 ± 1.3, 251 ± 1.8, and 249 ± 1.4 Ma, respectively. The granodiorites and granodiorite porphyry have low P2O5 (0.13–0.23 wt %) and A/CNK (0.79–1.05) values, and their SiO2 and P2O5 contents are negatively correlated, indicating I-type affinity. The positive εNd(t) values (+1.3 to +1.8) and young two-stage model ages (TDM2) (880–916 Ma) of the Baiyinnuoer intrusive rocks suggest that they might have formed by the mixing of both mantle and crustal materials. The variations in the major elements, Rb, Sr, and Ba, and the negative Nb–Ta–Ti anomalies indicate that fractional crystallization might have occurred during magma ascent. In combination with the regional geology, the new geochronological, geochemical, and isotopic data reveal that the ore-associated intrusive rocks at Baiyinnuoer were formed in a post-collision setting in the Late Permian.
Discovery of [1,2,4]Triazole Derivatives as New Metallo-β-Lactamase Inhibitors
Authors: Yuan Chen , Yan Jie , Song Chen , Yang Fan , Li Chao , Wang Cheng , Su Huiling , Chen Wei , Wang Lijiao , Wang Zhouyu , Qian Shan , Yang Lingling
Published: December 2019

The emergence and spread of metallo-β-lactamase (MBL)-mediated resistance to β-lactam antibacterials has already threatened the global public health. A clinically useful MBL inhibitor that can reverse β-lactam resistance has not been established yet. We here report a series of [1,2,4]triazole derivatives and analogs, which displayed inhibition to the clinically relevant subclass B1 (Verona integron-encoded MBL-2) VIM-2. 3-(4-Bromophenyl)-6,7-dihydro-5H-[1,2,4]triazolo [3,4-b][1,3]thiazine (5l) manifested the most potent inhibition with an IC50 (half-maximal inhibitory concentration) value of 38.36 μM. Investigations of 5l against other B1 MBLs and the serine β-lactamases (SBLs) revealed the selectivity to VIM-2. Molecular docking analyses suggested that 5l bound to the VIM-2 active site via the triazole involving zinc coordination and made hydrophobic interactions with the residues Phe61 and Tyr67 on the flexible L1 loop. This work provided new triazole-based MBL inhibitors and may aid efforts to develop new types of inhibitors combating MBL-mediated resistance.