bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly Efficient Production of DMF from Biomass-Derived HMF on Recyclable Ni-Fe/TiO2 Catalysts
Authors: Przydacz Martyna , Jędrzejczyk Marcin , Rogowski Jacek , Szynkowska-Jóźwik Małgorzata , Ruppert Agnieszka M.
Published: September 2020

5-Hydroxymethylfurfural, which can be derived from lignocellulosic biomass, can be transformed via the hydrodeoxygenation process to potential biofuels, such as 2,5 dimethylfuran or other chemicals of industrial importance. Non-noble metal catalysts constitute a robust and cheap solution for this process. In this work, the modification of the Ni/TiO2 catalyst by the addition of iron and support modification was evaluated. It was shown that bimetallic Ni-Fe catalysts are more selective and stable than their monometallic counterparts. This improvement in properties depends on the Ni:Fe ratio, but the support plays an equally important role—namely the high surface area anatase titania support improves the metal dispersion, resulting in a higher catalytic activity, and the formation of NiFe alloy facilitates the C=O bond cleavage. Such catalysts are active and stable and can be easily separated from the reaction mixture thanks to their magnetic properties.
Complex Investigation of High Efficiency and Reliable Heterojunction Solar Cell Based on an Improved Cu2O Absorber Layer
Authors: Fara Laurentiu , Chilibon Irinela , Nordseth Ørnulf , Craciunescu Dan , Savastru Dan , Vasiliu Cristina , Baschir Laurentiu , Fara Silvian , Kumar Raj , Monakhov Edouard , Connolly James P.
Published: September 2020

This study is aimed at increasing the performance and reliability of silicon-based heterojunction solar cells with advanced methods. This is achieved by a numerical electro-optical modeling and reliability analysis for such solar cells correlated with experimental analysis of the Cu2O absorber layer. It yields the optimization of a silicon tandem heterojunction solar cell based on a ZnO/Cu2O subcell and a c-Si bottom subcell using electro-optical numerical modeling. The buffer layer affinity and mobility together with a low conduction band offset for the heterojunction are discussed, as well as spectral properties of the device model. Experimental research of N-doped Cu2O thin films was dedicated to two main activities: (1) fabrication of specific samples by DC magnetron sputtering and (2) detailed characterization of the analyzed samples. This last investigation was based on advanced techniques: morphological (scanning electron microscopy—SEM and atomic force microscopy—AFM), structural (X-ray diffraction—XRD), and optical (spectroscopic ellipsometry—SE and Fourier-transform infrared spectroscopy—FTIR). This approach qualified the heterojunction solar cell based on cuprous oxide with nitrogen as an attractive candidate for high-performance solar devices. A reliability analysis based on Weibull statistical distribution establishes the degradation degree and failure rate of the studied solar cells under stress and under standard conditions.
A Real-Time Implementation of Novel and Stable Variable Step Size MPPT
Authors: Farhat Maissa , Barambones Oscar , Sbita Lassaâd
Published: September 2020

This paper presents a complete study of a standalone photovoltaic (PV) system including a maximum power tracker (MPPT) driving a DC boost converter to feed a resistive load. Here, a new MPPT approach using a modification on the original perturb and observe (P&O) algorithm is proposed; the improved algorithm is founded on a variable step size (VSZ). This novel algorithm is realized and efficiently implemented in the PV system. The proposed VSZ algorithm is compared both in simulation and in real time to the P&O algorithm. The stability analysis for the VSZ algorithm is performed using Lyapunov’s stability theory. In this paper, a detailed study and explanation of the modified P&O MPPT controller is presented to ensure high PV system performance. The proposed algorithm is practically implemented using a DSP1104 for real-time testing. Significant results are achieved, proving the validity of the proposed PV system control scheme. The obtained results show that the proposed VSZ succeeds at harvesting the maximum power point (MPP), as the amount of harvested power using VSZ is three times greater than the power extracted without the tracking algorithm. The VSZ reveals improved performance compared to the conventional P&O algorithm in term of dynamic response, signal quality and stability.
An Overview of Phytophthora Species Inhabiting Declining Quercus suber Stands in Sardinia (Italy)
Authors: Seddaiu Salvatore , Brandano Andrea , Ruiu Pino Angelo , Sechi Clizia , Scanu Bruno
Published: September 2020

Cork oak forests are of immense importance in terms of economic, cultural, and ecological value in the Mediterranean regions. Since the beginning of the 20th century, these forests ecosystems have been threatened by several factors, including human intervention, climate change, wildfires, pathogens, and pests. Several studies have demonstrated the primary role of the oomycete Phytophthora cinnamomi Ronds in the widespread decline of cork oaks in Portugal, Spain, southern France, and Italy, although other congeneric species have also been occasionally associated. Between 2015 and 2019, independent surveys were undertaken to determine the diversity of Phytophthora species in declining cork oak stands in Sardinia (Italy). Rhizosphere soil samples were collected from 39 declining cork oak stands and baited in the laboratory with oak leaflets. In addition, the occurrence of Phytophthora was assayed using an in-situ baiting technique in rivers and streams located throughout ten of the surveyed oak stands. Isolates were identified by means of both morphological characters and sequence analysis of internal transcribed spacer (ITS) regions of ribosomal DNA. In total, 14 different Phytophthora species were detected. Phytophthora cinnamomi was the most frequently isolated species from rhizosphere soil, followed by P. quercina, P. pseudocryptogea, and P. tyrrhenica. In contrast, P. gonapodyides turned out to be the most dominant species in stream water, followed by P. bilorbang, P. pseudocryptogea, P. lacustris, and P. plurivora. Pathogenicity of the most common Phytophthora species detected was tested using both soil infestation and log inoculation methods. This study showed the high diversity of Phytophthora species inhabiting soil and watercourses, including several previously unrecorded species potentially involved in the decline of cork oak forests.
Visible and Near-Infrared Multispectral Features in Conjunction with Artificial Neural Network and Partial Least Squares for Predicting Biochemical and Micro-Structural Features of Beef Muscles
Authors: Aït-Kaddour Abderrahmane , Andueza Donato , Dubost Annabelle , Roger Jean-Michel , Hocquette Jean-François , Listrat Anne
Published: September 2020

The objective of this study was to determine the potential of multispectral imaging (MSI) data recorded in the visible and near infrared electromagnetic regions to predict the structural features of intramuscular connective tissue, the proportion of intramuscular fat (IMF), and some characteristic parameters of muscle fibers involved in beef sensory quality. In order to do this, samples from three muscles (Longissimus thoracis, Semimembranosus and Biceps femoris) of animals belonging to three breeds (Aberdeen Angus, Limousine, and Blonde d’Aquitaine) were used (120 samples). After the acquisition of images by MSI and segmentation of their morphological parameters, a back propagation artificial neural network (ANN) model coupled with partial least squares was applied to predict the muscular parameters cited above. The results presented a high accuracy and are promising (R2 test > 0.90) for practical applications. For example, considering the prediction of IMF, the regression model giving the best ANN model exhibited R2P = 0.99 and RMSEP = 0.103 g × 100 g−1 DM.
The Effect of Barley and Lysine Supplementation on the longissimus lumborum Meat Quality of Pasture-Raised Fallow Deer (Dama dama)
Authors: Bureš Daniel , Bartoň Luděk , Kudrnáčová Eva , Kotrba Radim , Hoffman Louwrens C.
Published: September 2020

The chemical characteristics (proximate composition, amino acids, and fatty acids) and sensory quality of the longissimus lumborum (LL) muscle of 45 farmed male fallow deer were investigated. The animals were divided into three separate groups (n = 15 per treatment): pasture-fed (P), pasture-fed and supplemented with barley (B), and pasture-fed and supplemented with barley and lysine (BL). Differences were observed in LL moisture and the intramuscular fat contents, the latter being almost two-fold greater in the meat of B and BL groups compared to P. The concentrations of histidine, leucine, alanine, glutamic acid and glycine in the raw meat were higher in the BL group compared to the P group. Higher contents of n–3 polyunsaturated fatty acids (PUFAs), and consequently lower n–3 ratios, were found in the P group, compared to the BL group. The grilled meat samples from the P group scored higher than the other groups for grassy flavour, and lower for liver flavour.
The Mode of Grass Supply to Dairy Cows Impacts on Fatty Acid and Antioxidant Profile of Milk
Authors: De La Torre-Santos Senén , Royo Luis J. , Martínez-Fernández Adela , Chocarro Cristina , Vicente Fernando
Published: September 2020

The optimization of milk production includes a rational use of forages, respect for the environment and offers the best quality to consumers. Milk production based on grass and forages produces healthier milk and it is widely spread throughout the Atlantic arc to maximize milk yield per hectare. However, the mode of offering the grass can have a major influence on milk composition. The aim of this study was to evaluate the effect of grass supply mode (grazing, zero-grazing or ensiling) on dairy cows’ performance, with particular reference to fatty acids and fat-soluble antioxidants concentration. A three by three Latin square experiment was performed with 18 dairy cows. Experimental treatments consisted of exclusive feeding with grass silage and zero-grazing, both offered ad libitum indoors, or grazing for 24 h. The results showed that grazing cows had a higher dry matter intake and greater milk yield than cows feeding on grass silage and zero-grazing, as well as higher concentrations of protein, lactose, nonfat-solids and urea in milk than housed cows. Milk fat from grazing cows had a higher proportion of unsaturated fatty acids than from cows feeding on grass silage and zero-grazing, with significant differences in the proportion of vaccenic and rumenic acids. The 18:1 trans-11 to 18:1 trans-10 ratio is proposed as biomarker to identify the milk produced from the management system of grazing cattle. Milk from grazing cows had a greater proportion of lutein than cows eating grass silage, with the zero-grazing system having intermediate values. In conclusion, the mode of grass supply affects fatty acid and antioxidant profiles of milk.
Do Consumers Change Their Perception of Liking, Expected Satiety, and Healthiness of a Product If They Know It Is a Ready-to Eat Meal?
Authors: Laguna Laura , Gómez Beatriz , Garrido María D. , Fiszman Susana , Tarrega Amparo , Linares María B.
Published: September 2020

A ready-to-eat meal is a prepared meal within a container or package that requires little preparation or heating before consumption. Despite ready-to-eat meals being widely consumed, to date, little information is available on the consumers’ perceptions of such products in comparison to a homemade meal. Thus, three groups of eighty participants took part in the present study; each group evaluated five ready-to-eat meals (Pasta, Meatballs, Salad, Beans, and a Sandwich) using one of the following conditions: (i) observation of the packaging, (ii) observation of the meal on a plate (photographs), and (iii) tasting the ready-to-eat product with the packaging being presented alongside the meal. Consumers were asked about their liking, satiety, and healthiness perception. The results showed that both the ready-to-eat pack and sensory quality of the product highly impact liking and healthiness perceptions. Being a ready-to-eat meal in a pack has a negative impact on liking expectations of the meal; however, the sensory quality can either counteract these effects or increase them. Expected satiety of meals depends on the type of meal and varies slightly according to the evaluation condition.
Characterization of Differentially Expressed miRNAs and Their Predicted Target Transcripts during Smoltification and Adaptation to Seawater in Head Kidney of Atlantic Salmon
Authors: Shwe Alice , Østbye Tone-Kari Knutsdatter , Krasnov Aleksei , Ramberg Sigmund , Andreassen Rune
Published: September 2020

Smoltification and early seawater phase are critical developmental periods with physiological and biochemical changes in Atlantic salmon that facilitates survival in saltwater. MicroRNAs (miRNAs) are known to have important roles in development, but whether any miRNAs are involved in regulation of gene expression during smoltification and the adaption to seawater is largely unknown. Here, small RNA sequencing of materials from head kidney before, during smoltification and post seawater transfer were used to study expression dynamics of miRNAs, while microarray analysis was applied to study mRNA expression dynamics. Comparing all timepoints, 71 miRNAs and 2709 mRNAs were identified as differentially expressed (DE). Hierarchical clustering analysis of the DE miRNAs showed three major clusters with characteristic expression changes. Eighty-one DE mRNAs revealed negatively correlated expression patterns to DE miRNAs in Cluster I and III. Furthermore, 42 of these mRNAs were predicted as DE miRNA targets. Gene enrichment analysis of negatively correlated target genes showed they were enriched in gene ontology groups hormone biosynthesis, stress management, immune response, and ion transport. The results strongly indicate that post-transcriptional regulation of gene expression by miRNAs is important in smoltification and sea water adaption, and this study identifies several putative miRNA-target pairs for further functional studies.
Doing Motherhood, Doing Home: Mothering as Home-Making Practice in Half of a Yellow Sun
Authors: Jilek Barbara
Published: September 2020

Home and motherhood are tightly interwoven, particularly in the dominant conceptualizations of home as a physical and emotional refuge from the public world. However, a closer look into these concepts helps question the naturalization of both motherhood and home, revealing them as shaped by complex lived experiences and relations instead. I argue that such a rethinking of home and motherhood beyond essentialist discourse is prominent in Chimamanda Ngozi Adichie’s postcolonial novel Half of a Yellow Sun. Drawing on concepts and theories from the fields of gender studies and geography, and taking into account the postcolonial, Nigerian context of the novel, I address how Adichie’s 2006 piece of historical fiction thematizes the intersection point of motherhood and home as a relational practice. Adichie provides alternative conceptualizations of motherhood and home through her focus on performative, ritualized mothering practices that also function as relational home-making practices and that stretch beyond gender and biological relations. Through the central ambivalence that emerges in the novel when the female protagonist chooses and practices a traditional mother role but simultaneously does not correspond to the dominant Nigerian ideal of a mother, Adichie destabilizes binary views of both home and of motherhood.
Development and Evaluation of an Antiviral Agent Medication Adherence Education Program for Patients with Chronic Hepatitis C
Authors: Cho Hoo Jeung , Park Euna
Published: September 2020

This study aimed to develop and evaluate a goal attainment theory-based antiviral agent medication adherence education program (AMAEP) for patients with chronic hepatitis C. A nonequivalent control group pretest-posttest design was used. Data were collected from December 2019 to March 2020 from a control group of 35 outpatients and an experimental group of 28 outpatients older than 20 years old who had been diagnosed with chronic hepatitis C. The data analysis included an independent t-test, a χ2-test or Fisher’s exact test, a Kolmogorov–Smirnov test, an analysis of covariance, and a Mann–Whitney U test. The results showed the effectiveness of the education program for patients with chronic hepatitis C. There were significant differences between the control group and experimental group in patients’ knowledge of chronic hepatitis C (Z = −5.91, p < 0.001), medication self-efficacy (Z = −5.02, p < 0.001), medication adherence rate (t = −3.88, p < 0.001), medication misuse behavior (Z = −5.00, p < 0.001), and patients’ satisfaction with their interaction with healthcare practitioners (Z = −6.61, p < 0.001). Therefore, we hope that the education program developed in this study will be utilized as an intervention for patients with chronic hepatitis C and be further developed for other patients with viral hepatitis.
Psychometric Proprieties of a Mobile Application to Measure the Craniovertebral Angle a Validation and Reliability Study
Authors: Gallego-Izquierdo Tomas , Arroba-Díaz Enrique , García-Ascoz Gema , Val-Cano María del Alba , Pecos-Martin Daniel , Cano-de-la-Cuerda Roberto
Published: September 2020

The aim of this study was to assess the psychometric properties of the mobile application forward head posture in terms of validity, inter- and intra-rater reliability, minimum detectable change, sensitivity, and specificity to measure craniovertebral angle. In total, 44 subjects (mean age 23.30 ± 4.44 years) were evaluated in the standing position with markers on the tragus and cutaneous prominence of seventh cervical vertebra (C7). We had two experienced and trained physiotherapists assess cervical posture using the mobile application forward head posture and photogrammetry. Intraclass correlation coefficients were used to determine validity and reliability. A contingency table was made to determine sensitivity and specificity. Intra-rater reliability of the mobile application forward head posture had an intraclass correlation coefficient of 0.88. The inter-rater reliability generated an intraclass correlation coefficient of 0.83 to 0.89. Criterion validity data were above 0.82. The minimum detectable change was 4.96° for intra-rater and 5.52° for inter-rater reliability. The smartphone application exhibited 94.4% sensitivity and 84.6% specificity. The smartphone application forward head posture is a valid and reliable tool to measure craniovertebral angle in a standing position and, therefore, could be a useful assessment tool in clinical practice.
Strategies and Measurement Tools in Physical Activity Promotion Interventions in the University Setting: A Systematic Review
Authors: García-Álvarez David , Faubel Raquel
Published: September 2020

The university environment is especially suitable for implementing health promotion interventions and specifically for physical activity promotion among university students. The objective of this systematic review was to describe the strategies employed and the physical activity data collection tools that have been used in said interventions. A systematic search for articles was conducted using the PubMED, Cochrane, and PEDro databases. The articles selected were those describing a physical activity promotion intervention aimed at university students in their own university setting in which there was a control group. Eventually, 1074 articles were identified, of which 13 fulfilled the selection criteria. The results show eight strategies and nine different instruments for collecting physical activity data. The strategies identified were used in combination and they were adapted in each of the complex interventions. Validated questionnaires were the most widely used instrument. Future original studies are needed to find out the impact of these strategies in physical activity promotion among university students specifically in the university context.
Oral Health Inequalities and the Corporate Determinants of Health: A Commentary
Authors: Jamieson Lisa , Gibson Barry , Thomson W. Murray
Published: September 2020

Empirical research critically examining the role of the corporate determinants of health has gained traction in the past few years. Many of these reports have received strong, sometimes litigious, backlash from the corporations exposed. The aim of this paper is to provide a critical commentary on existing literature, policies, procedures and observations of issues, especially regarding the use of the corporate determinants of health as a research construct, in the persistence and flourishing of oral health inequalities at a global level. We discuss theoretical frameworks that underpin the power constructs of the corporate determinants of health, including Lukes “three faces of power” theory. This theory posits that power is exercised in three ways: through decision-making, through non-decision-making and ideologically. We will demonstrate, using examples of corporate determinants of health and oral health inequalities from several countries, how intervening at key leverage points is a crucial strategy for improving oral health inequalities at a global level.
A Perspective on Inhabited Urban Space: Land Use and Occupation, Heat Islands, and Precarious Urbanization as Determinants of Territorial Receptivity to Dengue in the City of Rio De Janeiro
Authors: Santos Jefferson Pereira Caldas , Honório Nildimar Alves , Barcellos Christovam , Nobre Aline Araújo
Published: September 2020

Introduction: Rio de Janeiro is the second-largest city in Brazil, with strong socio-spatial segregation, and diverse and heterogeneous land use, occupation, and landscapes. The complexity of dengue requires the construction of surveillance and control tools that take into account the historical, social, economic, and environmental processes mediated in the territory as a central axis of public policy. In this context, this study aimed to stratify the city into areas of receptivity to dengue, using innovative “territorial indicators” because they are built based on the actual occupation of the territory. Methods: We designed and constructed 17 indicators that sought to characterize the transformed and inhabited space according to receptivity to dengue. We used data on land use and occupation, connectivity, climate, and landscape. We developed the dengue receptivity through principal component analysis (PCA), using multiple criteria analysis and map algebra integrated in a GIS platform. Results: The most receptive areas were concentrated in the transition between the north and west zones of the city, a region of unconsolidated urban sprawl. The areas of greatest receptivity had the highest incidence and density of Aedes eggs during the study period. The correlation between receptivity index and incidence rate was positive in the epidemic years. Conclusion: The proposed set of indicators was able to identify areas of greater receptivity, such as regions of disorderly urban sprawl, with a concentration of social and environmental processes that are related to the occurrence of dengue outbreaks and high vector density. On the other hand, population immunity plays an important role in the spatial distribution of dengue during non-epidemic years.
Communicating Evidence about the Causes of Obesity and Support for Obesity Policies: Two Population-Based Survey Experiments
Authors: Reynolds James P. , Vasiljevic Milica , Pilling Mark , Hall Marissa G. , Ribisl Kurt M. , Marteau Theresa M.
Published: September 2020

Public support for numerous obesity policies is low, which is one barrier to their implementation. One reason for this low support is the tendency to ascribe obesity to failings of willpower as opposed to the environment. Correlational evidence supports this position. However, the experimental evidence is mixed. In two experimental studies, participants were randomised to receive no message, messages about the environment’s influence on obesity (Study 1 & 2), or messages about the environment’s influence on human behaviour (Study 1). We investigated whether communicating these messages changed support for obesity policies and beliefs about the causes of obesity. Participants were recruited from nationally representative samples in Great Britain (Study 1 & 2) and the USA (Study 2) (total n = 4391). Study 2 was designed to replicate existing research. Neither study found evidence that communicating the messages increased support for obesity policies or strengthened beliefs about the environment’s role in obesity. Study 2, therefore, did not replicate two earlier experimental studies. Instead, the studies reported here suggest that people’s beliefs about the causes of obesity are resistant to change in response to evidence and are, therefore, not a promising avenue to increase support for obesity policies.
Evaluation of the Reverse Mortgage Option in Korea: A Long Straddle Perspective
Authors: Choi Kyung Jin , Lim Byungkwon , Park Jaehwan
Published: September 2020

This study explored the option value embedded in a reverse mortgage in Korea through an empirical analysis, using the Black–Scholes option-pricing model. The value of a reverse mortgage is affected by the variation in house prices. However, older homeowners using reverse mortgages are able to choose this option due to the unique characteristics of reverse mortgages, such as non-recourse clauses or being able to redeem the loan. This paper found the following results. First, the call option value is 5.8% of the house price at the age of 60, under the assumption of a KRW three hundred million house value, while the put option value is only 2.0%. Contrary to what it is at sixty years of age, only the call option value will remain when the homeowner reaches the age of 80. Second, this article analyzed the sensitivity of the key variables of real-option analytical models, such as the change of the exercise price, the change of the risk-free rate, volatility, and maturity, on the option value of a reverse mortgage. The sensitivity results of the key variables supported economic rationales for the option pricing model.
Erratum: Ramos, F.; Trilles, S.; Torres-Sospedra, J.; Perales, F.J. New Trends in Using Augmented Reality Apps for Smart City Contexts. ISPRS Int. J. Geo-Inf. 2018, 7, 478
Authors: Yagol Pravesh , Ramos Francisco , Trilles Sergio , Torres-Sospedra Joaquín , Perales Francisco J.
Published: September 2020

The authors wish to make the following corrections to their paper [...]
Regional Terrain Complexity Assessment Based on Principal Component Analysis and Geographic Information System: A Case of Jiangxi Province, China
Authors: Huang Faming , Yang Jianbo , Zhang Biao , Li Yijing , Huang Jinsong , Chen Na
Published: September 2020

Regional terrain complexity assessment (TCA) is an important theoretical foundation for geological feature identification, hydrological information extraction and land resources utilization. However, the previous TCA models have many disadvantages; for example, comprehensive consideration and redundancy information analysis of terrain factors is lacking, and the terrain complexity index is difficult to quantify. To overcome these drawbacks, a TCA model based on principal component analysis (PCA) and a geographic information system (GIS) is proposed. Taking Jiangxi province of China as an example, firstly, ten terrain factors are extracted using a digital elevation model (DEM) in GIS software. Secondly, PCA is used to analyze the information redundancy of these terrain factors and deal with data compression. Then, the comprehensive evaluation of the compressed terrain factors is conducted to obtain quantitative terrain complexity indexes and a terrain complexity map (TCM). Finally, the TCM produced by the PCA method is compared with those produced by the slope-only, the variation coefficient and K-means clustering models based on the topographic map drawn by the Bureau of Land and Resources of Jiangxi province. Meanwhile, the TCM is also verified by the actual three-dimensional aerial images. Results show that the correlation coefficients between the TCMs produced by the PCA, slope-only, variable coefficient and K-means clustering models and the local topographic map are 0.894, 0.763, 0.816 and 0.788, respectively. It is concluded that the TCM of the PCA method matches well with the actual field terrain features, and the PCA method can reflect the regional terrain complexity characteristics more comprehensively and accurately when compared to the other three methods.
MicroRNAs in Chronic Kidney Disease: Four Candidates for Clinical Application
Authors: Peters Linsey J. F. , Floege Jürgen , Biessen Erik A. L. , Jankowski Joachim , van der Vorst Emiel P. C.
Published: September 2020

There are still major challenges regarding the early diagnosis and treatment of chronic kidney disease (CKD), which is in part due to the fact that its pathophysiology is very complex and not clarified in detail. The diagnosis of CKD commonly is made after kidney damage has occurred. This highlights the need for better mechanistic insight into CKD as well as improved clinical tools for both diagnosis and treatment. In the last decade, many studies have focused on microRNAs (miRs) as novel diagnostic tools or clinical targets. MiRs are small non-coding RNA molecules that are involved in post-transcriptional gene regulation and many have been studied in CKD. A wide array of pre-clinical and clinical studies have highlighted the potential role for miRs in the pathogenesis of hypertensive nephropathy, diabetic nephropathy, glomerulonephritis, kidney tubulointerstitial fibrosis, and some of the associated cardiovascular complications. In this review, we will provide an overview of the miRs studied in CKD, especially highlighting miR-103a-3p, miR-192-5p, the miR-29 family and miR-21-5p as these have the greatest potential to result in novel therapeutic and diagnostic strategies.
Muscle Wasting and Sarcopenia in Heart Failure—The Current State of Science
Authors: Lena Alessia , Anker Markus S. , Springer Jochen
Published: September 2020

Sarcopenia is primarily characterized by skeletal muscle disturbances such as loss of muscle mass, quality, strength, and physical performance. It is commonly seen in elderly patients with chronic diseases. The prevalence of sarcopenia in chronic heart failure (HF) patients amounts to up to 20% and may progress into cardiac cachexia. Muscle wasting is a strong predictor of frailty and reduced survival in HF patients. Despite many different techniques and clinical tests, there is still no broadly available gold standard for the diagnosis of sarcopenia. Resistance exercise and nutritional supplementation represent the currently most used strategies against wasting disorders. Ongoing research is investigating skeletal muscle mitochondrial dysfunction as a new possible target for pharmacological compounds. Novel agents such as synthetic ghrelin and selective androgen receptor modulators (SARMs) seem promising in counteracting muscle abnormalities but their effectiveness in HF patients has not been assessed yet. In the last decades, many advances have been accomplished but sarcopenia remains an underdiagnosed pathology and more efforts are needed to find an efficacious therapeutic plan. The purpose of this review is to illustrate the current knowledge in terms of pathogenesis, diagnosis, and treatment of sarcopenia in order to provide a better understanding of wasting disorders occurring in chronic heart failure.
Greatest Hits—Innovative Technologies for High Throughput Identification of Bispecific Antibodies
Authors: Hofmann Tim , Krah Simon , Sellmann Carolin , Zielonka Stefan , Doerner Achim
Published: September 2020

Recent years have shown a tremendous increase and diversification in antibody-based therapeutics with advances in production techniques and formats. The plethora of currently investigated bi- to multi-specific antibody architectures can be harnessed to elicit a broad variety of specific modes of actions in oncology and immunology, spanning from enhanced selectivity to effector cell recruitment, all of which cannot be addressed by monospecific antibodies. Despite continuously growing efforts and methodologies, the identification of an optimal bispecific antibody as the best possible combination of two parental monospecific binders, however, remains challenging, due to tedious cloning and production, often resulting in undesired extended development times and increased expenses. Although automated high throughput screening approaches have matured for pharmaceutical small molecule development, it was only recently that protein bioconjugation technologies have been developed for the facile generation of bispecific antibodies in a ‘plug and play’ manner. In this review, we provide an overview of the most relevant methodologies for bispecific screening purposes—the DuoBody concept, paired light chain single cell production approaches, Sortase A and Transglutaminase, the SpyTag/SpyCatcher system, and inteins—and elaborate on the benefits as well as drawbacks of the different technologies.
Chemopreventive Effect of Dietary Anthocyanins against Gastrointestinal Cancers: A Review of Recent Advances and Perspectives
Authors: Dharmawansa K.V. Surangi , Hoskin David W. , Rupasinghe H. P. Vasantha
Published: September 2020

Anthocyanins are a group of dietary polyphenols, abundant mainly in fruits and their products. Dietary interventions of anthocyanins are being studied extensively related to the prevention of gastrointestinal (GI) cancer, among many other chronic disorders. This review summarizes the hereditary and non-hereditary characteristics of GI cancers, chemistry, and bioavailability of anthocyanins, and the most recent findings of anthocyanin in GI cancer prevention through modulating cellular signaling pathways. GI cancer-preventive attributes of anthocyanins are primarily due to their antioxidative, anti-inflammatory, and anti-proliferative properties, and their ability to regulate gene expression and metabolic pathways, as well as induce the apoptosis of cancer cells.
Is There Evidence for IGF1R-Stimulating Abs in Graves’ Orbitopathy Pathogenesis?
Authors: Krieger Christine C. , Neumann Susanne , Gershengorn Marvin C.
Published: September 2020

In this review, we summarize the evidence against direct stimulation of insulin-like growth factor 1 receptors (IGF1Rs) by autoantibodies in Graves’ orbitopathy (GO) pathogenesis. We describe a model of thyroid-stimulating hormone (TSH) receptor (TSHR)/IGF1R crosstalk and present evidence that observations indicating IGF1R’s role in GO could be explained by this mechanism. We evaluate the evidence for and against IGF1R as a direct target of stimulating IGF1R antibodies (IGF1RAbs) and conclude that GO pathogenesis does not involve directly stimulating IGF1RAbs. We further conclude that the preponderance of evidence supports TSHR as the direct and only target of stimulating autoantibodies in GO and maintain that the TSHR should remain a major target for further development of a medical therapy for GO in concert with drugs that target TSHR/IGF1R crosstalk.
Characterization of Integrin αIIbβ3-Mediated Outside-in Signaling by Protein Kinase Cδ in Platelets
Authors: Chaudhary Preeti Kumari , Kim Sanggu , Jee Youngheun , Lee Seung-Hun , Kim Soochong
Published: September 2020

Engagement of integrin αIIbβ3 promotes platelet–platelet interaction and stimulates outside-in signaling that amplifies activation. Protein kinase Cδ (PKCδ) is known to play an important role in platelet activation, but its role in outside-in signaling has not been established. In the present study, we determined the role of PKCδ and its signaling pathways in integrin αIIbβ3-mediated outside-in signaling in platelets using PKCδ-deficient platelets. Platelet spreading to immobilized fibrinogen resulted in PKCδ phosphorylation, suggesting that αIIbβ3 activation caused PKCδ activation. αIIbβ3-mediated phosphorylation of Akt was significantly inhibited in PKCδ -/- platelets, indicating a role of PKCδ in outside-in signaling. αIIbβ3-mediated PKCδ phosphorylation was inhibited by proline-rich tyrosine kinase 2 (Pyk2) selective inhibitor, suggesting that Pyk2 contributes to the regulation of PKCδ phosphorylation in outside-in signaling. Additionally, Src-family kinase inhibitor PP2 inhibited integrin-mediated Pyk2 and PKCδ phosphorylation. Lastly, platelet spreading was inhibited in PKCδ -/- platelets compared to the wild-type (WT) platelets, and clot retraction from PKCδ -/- platelets was markedly delayed, indicating that PKCδ is involved in the regulation of αIIbβ3-dependent interactivities with cytoskeleton elements. Together, these results provide evidence that PKCδ plays an important role in outside-in signaling, which is regulated by Pyk2 in platelets.
Palaearctic Egg Parasitoids Interaction to Three Grapevine Exotic Pests in Northwestern Italy: A New Association Involving Metcalfa pruinosa
Authors: Bocca Federico Marco , Picciau Luca , Laudonia Stefania , Alma Alberto
Published: September 2020

The most important exotic leafhopper pests currently affecting the Italian vineyards are the leafhoppers Scaphoideus titanus, Orientus ishidae and the planthopper Metcalfa pruinosa. Their highest population density is detected in the uncultivated areas with wild grapevines. Should these habitats be considered only a problem or a potential resource for Palearctic entomophagy of these three exotic pests? The aim of this work was to study the biotopes and biocoenosis present in the Piedmontese vineyard agroecosystem, evaluating the parasitization rate and other crucial aspects for a possible application in biological control. Several specimens of egg-parasitoid wasps were obtained from filed-collected two-year-old grapevine canes. The most prevalent one belonged to the Oligosita collina group (Trichogrammatidae) emerged only from M. pruinosa eggs with a parasitization rate of over 40%. The new association is the first report of such a high level of parasitization on the flatid planthopper. The parasitization rate mainly relied on the host egg density and the abundance of plants suitable for the oviposition. A second parasitoid generation on the overwintering eggs is discussed, as well as other hypothesis. Furthermore, the parasitization rate was higher than the one showed by the dryinid Neodryinus typhlocybae, the control agent introduced in Italy under the biological control strategy, highlighting a possible implication in this biocoenosis. We assume that the egg parasitoid adaptation may contribute to M. pruinosa control.
First Report of Leptopilina japonica in Europe
Authors: Puppato Simone , Grassi Alberto , Pedrazzoli Federico , De Cristofaro Antonio , Ioriatti Claudio
Published: September 2020

Drosophila suzukii (Matsumura; Diptera: Drosophilidae) is a key pest of sweet cherry and small fruits worldwide. Biological control remains unutilized in the framework of D. suzukii management. Nonetheless, natural enemies may play an important role in regulating this pest. We report for the first time the presence of Leptopilina japonica Novković and Kimura (Hymenoptera: Figitidae) in Europe. Two specimens emerged from ripened fruits and one was collected after direct observation on a cherry tree in June 2019. They showed the distinctive morphological traits already described and shared more than 99% sequence similarity with specimens of L. japonica collected in Asia. This first finding was confirmed by a wider survey carried out in 2020; L. japonica emerged from cherry fruit samples collected in five other sites across the Trentino region, suggesting that L. japonica has already colonized a wide area. Detection of this Asian species is relevant to the future direction in managing D. suzukii, both in Europe and North America. In fact, L. japonica showed similarity with Ganaspis brasiliensis (Ihering) (Hymenoptera: Figitidae), the most promising candidate for the classical biological control, in terms of developmental time, egg maturation, host age preference and lifetime fecundity.
Time Trends of Crohn’s Disease in Catalonia from 2011 to 2017. Increasing Use of Biologics Correlates with a Reduced Need for Surgery
Authors: Brunet Eduard , Vela Emili , Melcarne Luigi , Clèries Montserrat , Pontes Caridad , Llovet Laura Patricia , García-Iglesias Pilar , Gallach Marta , Villòria Albert , Vergara Mercedes , Calvet Xavier
Published: September 2020

Background and Aims: Data from clinical trials suggest that biological drugs may improve the outcomes in Crohn’s disease (CD) by reducing the need for surgery or hospitalization. The aim of this study is to evaluate the time-trends of the use of biological drugs and other treatments for CD, and its relationship with outcomes in Catalonia. Materials and Methods: All patients with CD included in the Catalan Health Surveillance System (containing data on a population of more than 7.5 million) from 2011 to 2017 were identified. The exposures to different treatments for inflammatory bowel disease were retrieved from electronic invoicing records. Results: Between 2011 and 2017, the use of salicylates, corticosteroids and immunosuppressive treatment fell from 28.8% to 17.1%, 15.8% to 13.7%, and 32.9% to 29.6%, respectively (p < 0.001). Biological treatment use rose from 15.0% to 18.7% (p < 0.001). Ostomy rates per 1000 patients/year fell from 13.2 in 2011 to 9.8 in 2017 (p = 0.003), and surgical resection rates from 24.1 to 18.0 (p < 0.001). The rate of CD-related hospitalizations per 1000 patients/year also fell, from 92.7 to 72.2 (p < 0.001). Conclusions: Biological drug use rose from 15.0% to 18.7% between 2011 and 2017. During this period, we observed an improvement in the outcomes of CD patients.
Preoperative Neutrophil-to-Lymphocyte Ratio as a Prognostic Factor in Uterine Sarcoma
Authors: Jeong Min Jin , Park Jung Hyun , Hur Soo Young , Kim Chan Joo , Nam Hae Seong , Lee Yong Seok
Published: September 2020

Background: Recent studies have demonstrated that the tumor microenvironment, known to be influenced by inflammatory cells, plays a crucial role in cancer progression and clinical outcome of patients. The objective of the present study was to investigate prognostic values of preoperative neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) for disease-free survival (DFS) and overall survival (OS) of uterine sarcoma patients. Methods: Ninety-nine patients with uterine sarcoma treated in eight multicenter institutions over the last 20 years were retrospectively analyzed. Curves of DFS and OS were calculated using the Kaplan–Meier method, and univariate and multivariate analyses of various prognostic factors were performed using a Cox proportional hazard regression model. Results: High NLR was significantly associated with worse DFS (p = 0.007) and OS (p = 0.039). Advanced stage (p = 0.017) and high mitotic index (p = 0.036) retained their prognostic significance for DFS. Other clinical variables, including PLR, CA125, and lactate dehydrogenase (LDH) failed to show significant impact. Conclusions: Our findings showed that an elevated preoperative NLR was associated with poor clinical outcome in uterine sarcoma patients. Our results suggest that high NLR in early-stage uterine sarcoma patients might indicate that such patients need more intensive treatments.
Severe Acute Kidney Injury in Cardiovascular Surgery: Thrombotic Microangiopathy as a Differential Diagnosis to Ischemia Reperfusion Injury. A Retrospective Study
Authors: Grigorescu Melissa , Kamla Christine-Elena , Wassilowsky Dietmar , Joskowiak Dominik , Peterss Sven , Kemmner Stephan , Pichlmaier Maximilian , Hagl Christian , Fischereder Michael , Schönermarck Ulf
Published: September 2020

Background: Acute kidney injury (AKI) after cardiovascular surgery (CVS) infers high morbidity and mortality and may be caused by thrombotic microangiopathy (TMA). This study aimed to assess incidence, risk factors, kidney function, and mortality of patients with a postoperative TMA as possible cause of severe AKI following cardiovascular surgery. Methods: We analyzed retrospectively all patients admitted to the ICU after a cardiovascular procedure between 01/2018 and 03/2019 with severe AKI and need for renal replacement therapy (RRT). TMA was defined as post-surgery-AKI including need for RRT, hemolytic anemia, and thrombocytopenia. TMA patients were compared to patients with AKI requiring RRT without TMA. Results: Out of 893 patients, 69 (7.7%) needed RRT within one week after surgery due to severe AKI. Among those, 15 (21.7%) fulfilled TMA criteria. Aortic surgery suggested an increased risk for TMA (9/15 (60.0%) vs. 7/54 (31.5%), OR 3.26, CI 1.0013-10.64). Ten TMA patients required plasmapheresis and/or eculizumab, and five recovered spontaneously. Preoperative kidney function was significantly better in TMA patients than in controls (eGFR 92 vs. 60.5 mL/min, p = 0.004). However, postoperative TMA resulted in a more pronounced GFR loss (ΔeGFR −54 vs. −17 mL/min, p = 0.062). There were no deaths in the TMA group. Conclusion: Our findings suggest TMA as an important differential diagnosis of severe AKI following cardiovascular surgery, which may be triggered by aortic surgery. Therefore, early diagnosis and timely treatment of TMA could reduce kidney damage and improve mortality of AKI following cardiovascular surgery, which should be further investigated.
Heterogeneity of Clinical Presentations and Paraclinical Explorations to Diagnose Disseminated Histoplasmosis in Patients with Advanced HIV: 34 Years of Experience in French Guiana
Authors: Nacher Mathieu , Valdes Audrey , Adenis Antoine , Blaizot Romain , Abboud Philippe , Demar Magalie , Djossou Félix , Epelboin Loïc , Drak Alsibai Kinan , Misslin Caroline , Ntab Balthazar , Couppié Pierre
Published: September 2020

We aimed to describe the ways patients with disseminated histoplasmosis—a multifaceted and often lethal disease—present themselves and are explored. A retrospective, observational, multicentric study spanned the period between 1 January 1981 and 1 October 2014. Principal component analysis was performed for the sampling sites and for the clinical signs and symptoms. The factor loadings of the principal components were selected for eigenvalues > 1. The most frequent signs and symptoms were an alteration of the WHO general performance status, fever, digestive tract, respiratory signs and symptoms and lymphadenopathies. The most common sites sampled were bone marrow, respiratory tract, blood, lymph node and liver biopsies, with significant variations in the number of sites from which samples were taken to try to identify the pathogen. The principal component analysis clinical signs and symptoms leading to the diagnosis showed four main lines of variation. The factor loadings of the four main components were compatible with four broad types of clinical presentations and four types of exploration strategies. Extracting simple algorithms was difficult, emphasizing the importance of clinical expertise when diagnosis depends on obtaining a sample where Histoplasma can be seen or grown. Histoplasma antigen detection tests will help simplifying the algorithms.