bluuebunny/crossref_metadata_2025 huggingface dataset:
Geostatistical Distribution and Contamination Status of Heavy Metals in the Sediment of Perak River, Malaysia
Authors: Salam Mohammed Abdus , Paul Shujit Chandra , Shaari Farrah Izzaty , Rak Aweng Eh , Ahmad Rozita Binti , Kadir Wan Rashidah
Published: April 2019

Heavy metal pollution is one of the major environmental issues in recent decades owing to the rapid increase in urbanisation and industrialisation. Sediments usually act as sinks for heavy metals due to their complex physical and chemical adsorption mechanisms. In this study, heavy metals like lead (Pb), Zinc (Zn), Cadmium (Cd), Copper (Cu) and Iron (Fe) in the surface sediment from 15 location (upstream and downstream) on the Perak River, Malaysia were investigated by means of inductively coupled plasma optical emission spectroscopy (ICP-OES). The geostatistical prediction map showed the range of Pb, Zn, Cd, Cu and Fe concentration in upstream area was 14.56–27.0 µg/g, 20–51.27 µg/g, 1.51–3.0 µg/g, 6.6–19.12 µg/g and 20.24–56.58%, respectively, and in downstream areas was 27.6–60.76 µg/g, 49.04–160.5 µg/g, 2.77–4.02 µg/g, 9.82–59.99 µg/g and 31.34–39.5%, respectively. Based on the enrichment factor and geoaccumulation index, Cd was found to be the most dominant pollutant in the study area. Pollution load index, sediment quality guidelines and sediment environmental toxicity quotient data showed that the downstream sediment was more polluted than the upstream sediment in the Perak River. The multivariate analysis showed that Pb, Zn and Cu mainly originated from natural sources with minor contribution from human activities, whereas Fe and Cd originated from various industrial and agricultural activities along the studied area.
PM2.5 Cooperative Control with Fuzzy Cost and Fuzzy Coalitions
Authors: Zhou Zhen , Zhang Meijia , Yu Xiaohui , He Xijun , Wang Kang , Shao Quan , Wang Jie , Sun Hongxia
Published: April 2019

Haze control cost is hard to value by a crisp number because it is often affected by various factors such as regional uncertain meteorological conditions and topographical features. Furthermore, regions may be involved in different coalitions for haze control with different levels of effort. In this paper, we propose a PM2.5 cooperative control model with fuzzy cost and crisp coalitions or fuzzy coalitions based on the uncertain cross-border transmission factor. We focus on the Beijing–Tianjin–Hebei regions of China and obtain the following major findings. In the case of haze control in the Beijing–Tianjin–Hebei regions of China, local governments in the global crisp coalition can achieve their emission reduction targets with the lowest aggregated cost. However, Hebei fails to satisfy its individual rationality if there is no cost sharing. Therefore, the Hukuhara–Shapley value is used to allocate the aggregated cost among these regions so that the grand coalition is stable. However, the Beijing–Tianjin–Hebei regions cannot achieve their emission reduction targets in the global fuzzy coalition without government subsidies.
Public Perception on Healthcare Services: Evidence from Social Media Platforms in China
Authors: Hu Guangyu , Han Xueyan , Zhou Huixuan , Liu Yuanli
Published: April 2019

Social media has been used as data resource in a growing number of health-related research. The objectives of this study were to identify content volume and sentiment polarity of social media records relevant to healthcare services in China. A list of the key words of healthcare services were used to extract data from WeChat and Qzone, between June 2017 and September 2017. The data were put into a corpus, where content analyses were performed using Tencent natural language processing (NLP). The final corpus contained approximately 29 million records. Records on patient safety were the most frequently mentioned topic (approximately 8.73 million, 30.1% of the corpus), with the contents on humanistic care having received the least social media references (0.43 Million, 1.5%). Sentiment analyses showed 36.1%, 16.4%, and 47.4% of positive, neutral, and negative emotions, respectively. The doctor-patient relationship category had the highest proportion of negative contents (74.9%), followed by service efficiency (59.5%), and nursing service (53.0%). Neutral disposition was found to be the highest (30.4%) in the contents on appointment-booking services. This study added evidence to the magnitude and direction of public perceptions on healthcare services in China’s hospital and pointed to the possibility of monitoring healthcare service improvement, using readily available data in social media.
A Land Use/Land Cover Based Green Development Study for Different Functional Regions in the Jiangsu Province, China
Authors: Guo Xiaomin , Chuai Xiaowei , Huang Xianjin
Published: April 2019

Land use/land cover (LULC) change can strongly affect carbon storage in terrestrial ecosystems. The rapid development of China’s economy has formed different functional regions. These functional regions profoundly affect land use patterns. Thus, assessing the carbon storage induced by LULC changes is significant for green development. Selecting the typical region of the Jiangsu Province as the study area, this study first examines the research associated with the regional functional characteristics and various high accuracy data and methods have been used to greatly improve the research accuracy. The results showed that from 1995 to 2015, approximately 10.26% of the entire land area had LULC type changes. Additionally, decreases in the built-up land expansion and ecological land were the main LULC change characteristics, which are mainly affected by socioeconomic development. The total carbon storage of the Jiangsu Province decreased by 714.03 × 104 t and the four regions all presented decreasing carbon storage levels. The economically developed regions presented a more obvious loss of carbon. The region with small LULC changes had a lower carbon loss. The land transfer of cultivated land to built-up land is the main transfer type causing the carbon storage loss. This study investigates the human-environmental interactions from the new perspective of functional zoning and, thus, it enriches the comparative analysis of carbon storage in functional regions and provides references for the green development of a developing country’s developed areas.
Family Functioning and Psychological Health of Children with Mentally Ill Parents
Authors: Wiegand-Grefe Silke , Sell Marlit , Filter Bonnie , Plass-Christl Angela
Published: April 2019

Parental mental illness can be linked to reduced family functioning, which is associated with more conflicts, less adaptability and cohesion as well as a disorganized pattern of everyday planning. Concurrently, family functioning is an important moderator for the influence of parental mental disorders on the development of the children. Consequently, the current study addresses the correlation of family functioning in families with mentally ill parents and the psychological health of the children. The sample consists of 67 mentally ill parents. Both parents and therapists completed questionnaires related to family functioning and the psychological health of the children. Family functioning was rated as dysfunctional in 38% of the families. The psychological health of the children was classified as clinical or subclinical in 43% of the cases. 52% of the children were rated to have no psychological problems. In families with good family functioning, children were assessed to have less psychological problems than in families with poor functioning. Children outside the clinical range lived in families with good family functioning and vice versa. Significant positive correlations were found between the FB-A scales, the CBCL/4-18 syndrome scales and the CBCL/4–18 total score. Results indicate that family functioning and psychological health of children in families with mentally ill parents correlate closely and represent potential targets for future family interventions.
Risks of Ear Complaints of Passengers and Drivers While Trains Are Passing Through Tunnels at High Speed: A Numerical Simulation and Experimental Study
Authors: Xie Pengpeng , Peng Yong , Wang Tiantian , Zhang Honghao
Published: April 2019

Ear complaints induced by interior pressure transients are common experiences for passengers and crew members when high-speed trains are passing through tunnels. However, approaches to assessing the risks of the pressure-related aural discomfort have not been reported until recently. The objective of this study was to evaluate the hazards of interior pressure transients of high-speed train on human ears combining the effects of operation speed and seal index. Moving model tests were conducted to obtain the pressure transients when the model train runs in the tunnel. The recorded data were transformed into the interior pressures by empirical formula. Furthermore, the aural sensations were divided into four levels hierarchically and the range for each level was derived by logistic regression analysis method and represented by three biomechanical metrics. Furthermore, a human middle ear finite element (FE) model was used to simulate its dynamics under the interior pressures. The results indicate that lifting operation speed from 250 km/h to 350 km/h in tunnel will prolong the duration of ear complaints by more than two times whereas improving the seal index from 4 s to 12 s will reduce the incidences of the onset of tinnitus and hearing loss by more than ten times. In addition, the duration of aural comfort shortens from the head car to the tail car against the running direction. It is desirable that enhancing the seal index improve the aural sensations of the passengers and crew members considering the lifting operation speed of high-speed train.
Going beyond Quietness: Determining the Emotionally Restorative Effect of Acoustic Environments in Urban Open Public Spaces
Authors: Herranz-Pascual Karmele , Aspuru Itziar , Iraurgi Ioseba , Santander Álvaro , Eguiguren Jose Luis , García Igone
Published: April 2019

The capacity of natural settings to promote psychological restoration has attracted increasing research attention, especially with regards to the visual dimension. However, there is a need to extend these studies to urban settings, such as squares, parks or gardens, due to the global trend towards urbanisation, and to integrate the dimension of sound into landscape. Such was the main aim of this study, in which 53 participants assessed four public spaces in Vitoria-Gasteiz (Spain) as part of the CITI-SENSE Project (137 observations were used for analysis). A smartphone application was used to simultaneously collect objective and subjective data. The results show that at the end of the urban environmental experience, there was a statistically significant reduction in negative emotions and perceived stress, and a slight increase in positive emotions. Emotional restoration was mainly associated with prior emotional states, but also with global environmental comfort and acoustic comfort. The soundscape characteristics that contributed to greater emotional restoration and a reduction in perceived stress were pleasantness, calm, fun and naturalness. Therefore, in agreement with previous research, the findings of the present study indicate that besides contributing to the quietness of the urban environment, the urban soundscape can promote psychological restoration in users of these spaces.
Melkersson–Rosenthal Syndrome in Childhood: Report of Three Paediatric Cases and a Review of the Literature
Authors: Savasta Salvatore , Rossi Alessandra , Foiadelli Thomas , Licari Amelia , Elena Perini Anna Maria , Farello Giovanni , Verrotti Alberto , Marseglia Gian Luigi
Published: April 2019

Melkersson–Rosenthal syndrome (MRS) in children is a rare condition, clinically characterised by a triad of synchronous or metachronous symptoms: recurrent peripheral facial palsy, relapsing orofacial oedema, and a fissured tongue; the most recent review published on the topic has reported 30 published patients. The aetiology of this disease is still unclear. However, genetic factors, as well as alterations in immune functions, infections, and allergic reactions have been postulated. We report three children suffering from MRS and perform a literature review of paediatric cases. Taking into account that clinical and laboratoristical criteria for the diagnosis of MRS are lacking, this syndrome is probably underestimated, and we suggest increasing awareness of such a rare syndrome. Close multidisciplinary follow-up of these children with a team composed by paediatricians, neurologists, neuro-ophthalmologists, dermatologists, and otolaryngologists is crucial to guarantee exhaustive management and treatment success, while minimising relapses.
On the Feasibility of Water Surface Mapping with Single Photon LiDAR
Authors: Mandlburger Gottfried , Jutzi Boris
Published: April 2019

Single photon sensitive airborne Light Detection And Ranging (LiDAR) enables a higher area performance at the price of an increased outlier rate and a lower ranging accuracy compared to conventional Multi-Photon LiDAR. Single Photon LiDAR, in particular, uses green laser light potentially capable of penetrating clear shallow water. The technology is designed for large-area topographic mapping, which also includes the water surface. While the penetration capabilities of green lasers generally lead to underestimation of the water level heights, we specifically focus on the questions of whether Single Photon LiDAR (i) is less affected in this respect due to the high receiver sensitivity, and (ii) consequently delivers sufficient water surface echoes for precise high-resolution water surface reconstruction. After a review of the underlying sensor technology and the interaction of green laser light with water, we address the topic by comparing the surface responses of actual Single Photon LiDAR and Multi-Photon Topo-Bathymetric LiDAR datasets for selected horizontal water surfaces. The anticipated superiority of Single Photon LiDAR could not be verified in this study. While the mean deviations from a reference water level are less than 5 cm for surface models with a cell size of 10 m, systematic water level underestimation of 5–20 cm was observed for high-resolution Single Photon LiDAR based water surface models with cell sizes of 1–5 m. Theoretical photon counts obtained from simulations based on the laser-radar equation support the experimental data evaluation results and furthermore confirm the feasibility of Single Photon LiDAR based high-resolution water surface mapping when adopting specifically tailored flight mission parameters.
A New Approach for Glyco-Functionalization of Collagen-Based Biomaterials
Authors: Figuereido Ines , Paiotta Alice , Dal Magro Roberta , Tinelli Francesca , Corti Roberta , Re Francesca , Cassina Valeria , Caneva Enrico , Nicotra Francesco , Russo Laura
Published: April 2019

The cell microenvironment plays a pivotal role in mediating cell adhesion, survival, and proliferation in physiological and pathological states. The relevance of extracellular matrix (ECM) proteins in cell fate control is an important issue to take into consideration for both tissue engineering and cell biology studies. The glycosylation of ECM proteins remains, however, largely unexplored. In order to investigate the physio-pathological effects of differential ECM glycosylation, the design of affordable chemoselective methods for ECM components glycosylation is desirable. We will describe a new chemoselective glycosylation approach exploitable in aqueous media and on non-protected substrates, allowing rapid access to glyco-functionalized biomaterials.
The Potential Role of iNOS in Ovarian Cancer Progression and Chemoresistance
Authors: Kielbik Michal , Szulc-Kielbik Izabela , Klink Magdalena
Published: April 2019

Inducible nitric oxide synthase (iNOS), the enzyme responsible for nitric oxide (NO) production, is not present in most cells under normal conditions. The expression of its mRNA, as well as its protein synthesis and full enzymatic activity, undergoes multilevel regulation including transcriptional and posttranscriptional mechanisms, the availability of iNOS substrate and cofactors and oxygen tension. However, in various malignant diseases, such as ovarian cancer, the intracellular mechanisms controlling iNOS are dysregulated, resulting in the permanent induction of iNOS expression and activation. The present review summarizes the multistaged processes occurring in normal cells that promote NO synthesis and focuses on factors regulating iNOS expression in ovarian cancer. The possible involvement of iNOS in the chemoresistance of ovarian cancer and its potential as a prognostic/predictive factor in the course of disease development are also reviewed. According to the available yet limited data, it is difficult to draw unequivocal conclusions on the pros and cons of iNOS in ovarian cancer. Most clinical data support the hypothesis that high levels of iNOS expression in ovarian tumors are associated with a greater risk of disease relapse and patient death. However, in vitro studies with various ovarian cancer cell lines indicate a correlation between a high level of iNOS expression and sensitivity to cisplatin.
Fibrin Sealant Derived from Human Plasma as a Scaffold for Bone Grafts Associated with Photobiomodulation Therapy
Authors: Pomini Karina Torres , Buchaim Daniela Vieira , Andreo Jesus Carlos , Rosso Marcelie Priscila de Oliveira , Della Coletta Bruna Botteon , German Íris Jasmin Santos , Biguetti Ana Carolina Cestari , Shinohara André Luis , Rosa Júnior Geraldo Marco , Cosin Shindo João Vitor Tadashi , Alcalde Murilo Priori , Duarte Marco Antônio Hungaro , de Bortoli Teixeira Daniel , Buchaim Rogério Leone
Published: April 2019

Fibrin sealants derived from human blood can be used in tissue engineering to assist in the repair of bone defects. The objective of this study was to evaluate the support system formed by a xenograft fibrin sealant associated with photobiomodulation therapy of critical defects in rat calvaria. Thirty-six rats were divided into four groups: BC (n = 8), defect filled with blood clot; FSB (n = 10), filled with fibrin sealant and xenograft; BCPBMT (n = 8), blood clot and photobiomodulation; FSBPBMT (n = 10), fibrin sealant, xenograft, and photobiomodulation. The animals were killed after 14 and 42 days. In the histological and microtomographic analysis, new bone formation was observed in all groups, limited to the defect margins, and without complete wound closure. In the FSB group, bone formation increased between periods (4.3 ± 0.46 to 6.01 ± 0.32), yet with lower volume density when compared to the FSBPBMT (5.6 ± 0.45 to 10.64 ± 0.97) group. It was concluded that the support system formed by the xenograft fibrin sealant associated with the photobiomodulation therapy protocol had a positive effect on the bone repair process.
Effects of Palm Oil Tocotrienol-Rich Fraction (TRF) and Carotenes in Ovalbumin (OVA)-Challenged Asthmatic Brown Norway Rats
Authors: Zainal Zaida , Abdul Rahim Afiqah , Khaza’ai Huzwah , Chang Sui
Published: April 2019

Synthetic therapeutic drugs for asthma, a chronic airway inflammation characterised by strong eosinophil, mast cell, and lymphocyte infiltration, mucus hyper-production, and airway hyper-responsiveness, exhibit numerous side effects. Alternatively, the high antioxidant potential of palm oil phytonutrients, including vitamin E (tocotrienol-rich fractions; TRF) and carotene, may be beneficial for alleviating asthma. Here, we determined the therapeutic efficacy of TRF, carotene, and dexamethasone in ovalbumin-challenged allergic asthma in Brown Norway rats. Asthmatic symptoms fully developed within 8 days after the second sensitization, and were preserved throughout the time course via intranasal ovalbumin re-challenge. Asthmatic rats were then orally administered 30 mg/kg body weight TRF or carotene. TRF-treated animals exhibited reduced inflammatory cells in bronchial alveolar lavage fluid. TRF- and carotene-treated rats exhibited notable white blood cell reduction comparable to that from dexamethasone. TRF- and carotene-treatment also downregulated pro-inflammatory markers (IL-β, IL-6, TNF-α), coincident with anti-inflammatory marker IL-4 and IL-13 upregulation. Treatment significantly reduced asthmatic rat plasma CRP and IgE, signifying improved systemic inflammation. Asthmatic lung histology displayed severe edema and inflammatory cell infiltration in the bronchial wall, whereas treated animals retained healthy, normal-appearing lungs. The phytonutrients tocotrienol and carotene thus exhibit potential benefits for consumption as nutritional adjuncts in asthmatic disease.
Angiotensin II Induces Automatic Activity of the Isolated Guinea Pig Pulmonary Vein Myocardium through Activation of the IP3 Receptor and the Na+-Ca2+ Exchanger
Authors: Tanaka Yusuke , Obata Kae , Ohmori Tamano , Ishiwata Kohei , Abe Manato , Hamaguchi Shogo , Namekata Iyuki , Tanaka Hikaru
Published: April 2019

The automaticity of the pulmonary vein myocardium is known to be the major cause of atrial fibrillation. We examined the involvement of angiotensin II in the automatic activity of isolated guinea pig pulmonary vein preparations. In tissue preparations, application of angiotensin II induced an automatic contractile activity; this effect was mimicked by angiotensin I and blocked by losartan, but not by PD123,319 or carvedilol. In cardiomyocytes, application of angiotensin II induced an increase in the frequency of spontaneous Ca2+ sparks and the generation of Ca2+ transients; these effects were inhibited by losartan or xestospongin C. In tissue preparations, angiotensin II caused membrane potential oscillations, which lead to repetitive generation of action potentials. Angiotensin II increased the diastolic depolarization slope of the spontaneous or evoked action potentials. These effects of angiotensin II were inhibited by SEA0400. In tissue preparations showing spontaneous firing of action potentials, losartan, xestospongin C or SEA0400 decreased the slope of the diastolic depolarization and inhibited the firing of action potentials. In conclusion, in the guinea pig pulmonary vein myocardium, angiotensin II induces the generation of automatic activity through activation of the IP3 receptor and the Na+-Ca2+ exchanger.
Roles of the Two-MnSOD System of Stenotrophomonas maltophilia in the Alleviation of Superoxide Stress
Authors: Jair Herng-Woei , Lu Hsu-Feng , Huang Yi-Wei , Pan Sz-Yun , Lin I-Ling , Huang Hsin-Hui , Yang Tsuey-Ching
Published: April 2019

Manganese-dependent superoxide dismutase (MnSOD, SodA) and iron-dependent SOD (FeSOD, SodB) are critical cytosolic enzymes for alleviating superoxide stress. Distinct from the singular sodA gene in most bacteria, Stenotrophomonas maltophilia harbors two sodA genes, sodA1 and sodA2. The roles of SodA1, SodA2, and SodB of S. maltophilia in alleviating superoxide stress were investigated. The expression of sod genes was determined by promoter–xylE transcriptional fusion assay and qRT–PCR. SodA2 and sodB expressions were proportional to the bacterial logarithmic growth, but unaffected by menadione (MD), iron, or manganese challenges. SodA1 was intrinsically unexpressed and inducibly expressed by MD. Complementary expression of sodA1 was observed when sodA2 was inactivated. The individual or combined sod deletion mutants were constructed using the gene replacement strategy. The functions of SODs were assessed by evaluating cell viabilities of different sod mutants in MD, low iron-stressed, and/or low manganese-stressed conditions. Inactivation of SodA1 or SodA2 alone did not affect bacterial viability; however, simultaneously inactivating sodA1 and sodA2 significantly compromised bacterial viability in both aerobic growth and stressed conditions. SodA1 can either rescue or support SodA2 when SodA2 is defective or insufficiently potent. The presence of two MnSODs gives S. maltophilia an advantage against superoxide stress.
Cyclooxygenase-2 Activity Regulates Recruitment of VEGF-Secreting Ly6Chigh Monocytes in Ventilator-Induced Lung Injury
Authors: Huang Tzu-Hsiung , Fang Pin-Hui , Li Jhy-Ming , Ling Huan-Yuan , Lin Chieh-Mo , Shi Chung-Sheng
Published: April 2019

Mechanical ventilation is usually required for saving lives in critically ill patients; however, it can cause ventilator-induced lung injury (VILI). As VEGF-secreting Ly6Chigh monocytes are involved in VILI pathogenesis, we investigated whether cyclooxygenase-2 (COX-2) activity regulates the recruitment of VEGF-secreting Ly6Chigh monocytes during VILI. The clinically relevant two-hit mouse model of VILI, which involves the intravenous injection of lipopolysaccharide prior to high tidal volume (HTV)-mechanical ventilation, was used in this study. To investigate the role of COX-2 in the recruitment of VEGF-secreting Ly6Chigh monocytes during VILI, celecoxib, which is a clinical COX-2 inhibitor, was administered 1 h prior to HTV-mechanical ventilation. Pulmonary vascular permeability and leakage, inflammatory leukocyte infiltration, and lung oxygenation levels were measured to assess the severity of VILI. HTV-mechanical ventilation significantly increased the recruitment of COX-2-expressing Ly6Chigh, but not Ly6Clow, monocytes. Celecoxib significantly diminished the recruitment of Ly6Chigh monocytes, attenuated the levels of VEGF and total protein in bronchoalveolar lavage fluid, and restored pulmonary oxygenation during VILI. Our findings demonstrate that COX-2 activity is important in the recruitment of VEGF-secreting Ly6Chigh monocytes, which are involved in VILI pathogenesis, and indicate that the suppression of COX-2 activity might be a useful strategy in mitigating VILI.
Two Component Regulatory Systems and Antibiotic Resistance in Gram-Negative Pathogens
Authors: Bhagirath Anjali Y. , Li Yanqi , Patidar Rakesh , Yerex Katherine , Ma Xiaoxue , Kumar Ayush , Duan Kangmin
Published: April 2019

Gram-negative pathogens such as Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa are the leading cause of nosocomial infections throughout the world. One commonality shared among these pathogens is their ubiquitous presence, robust host-colonization and most importantly, resistance to antibiotics. A significant number of two-component systems (TCSs) exist in these pathogens, which are involved in regulation of gene expression in response to environmental signals such as antibiotic exposure. While the development of antimicrobial resistance is a complex phenomenon, it has been shown that TCSs are involved in sensing antibiotics and regulating genes associated with antibiotic resistance. In this review, we aim to interpret current knowledge about the signaling mechanisms of TCSs in these three pathogenic bacteria. We further attempt to answer questions about the role of TCSs in antimicrobial resistance. We will also briefly discuss how specific two-component systems present in K. pneumoniae, A. baumannii, and P. aeruginosa may serve as potential therapeutic targets.
Mechanisms of Adiponectin Action: Implication of Adiponectin Receptor Agonism in Diabetic Kidney Disease
Authors: Kim Yaeni , Park Cheol Whee
Published: April 2019

Adiponectin, an adipokine secreted by adipocytes, exerts favorable effects in the milieu of diabetes and metabolic syndrome through its anti-inflammatory, antifibrotic, and antioxidant effects. It mediates fatty acid metabolism by inducing AMP-activated protein kinase (AMPK) phosphorylation and increasing peroxisome proliferative-activated receptor (PPAR)-α expression through adiponectin receptor (AdipoR)1 and AdipoR2, respectively, which in turn activate PPAR gamma coactivator 1 alpha (PGC-1α), increase the phosphorylation of acyl CoA oxidase, and upregulate the uncoupling proteins involved in energy consumption. Moreover, adiponectin potently stimulates ceramidase activity associated with its two receptors and enhances ceramide catabolism and the formation of its anti-apoptotic metabolite, sphingosine 1 phosphate (S1P), independently of AMPK. Low circulating adiponectin levels in obese patients with a risk of insulin resistance, type 2 diabetes, and cardiovascular diseases, and increased adiponectin expression in the state of albuminuria suggest a protective and compensatory role for adiponectin in mitigating further renal injury during the development of overt diabetic kidney disease (DKD). We propose AdipoRon, an orally active synthetic adiponectin receptor agonist as a promising drug for restoration of DKD without inducing systemic adverse effects. Its renoprotective role against lipotoxicity and oxidative stress by enhancing the AMPK/PPARα pathway and ceramidase activity through AdipoRs is revealed here.
Empowering Mesenchymal Stem Cells for Ocular Degenerative Disorders
Authors: Ding Shirley Suet Lee , Subbiah Suresh Kumar , Khan Mohammed Safwan Ali , Farhana Aisha , Mok Pooi Ling
Published: April 2019

Multipotent mesenchymal stem cells (MSCs) have been employed in numerous pre-clinical and clinical settings for various diseases. MSCs have been used in treating degenerative disorders pertaining to the eye, for example, age-related macular degeneration, glaucoma, retinitis pigmentosa, diabetic retinopathy, and optic neuritis. Despite the known therapeutic role and mechanisms of MSCs, low cell precision towards the targeted area and cell survivability at tissue needing repair often resulted in a disparity in therapeutic outcomes. In this review, we will discuss the current and feasible strategy options to enhance treatment outcomes with MSC therapy. We will review the application of various types of biomaterials and advances in nanotechnology, which have been employed on MSCs to augment cellular function and differentiation for improving treatment of visual functions. In addition, several modes of gene delivery into MSCs and the types of associated therapeutic genes that are important for modulation of ocular tissue function and repair will be highlighted.
Mitogen Activated Protein Kinases in Steatotic and Non-Steatotic Livers Submitted to Ischemia-Reperfusion
Authors: Jiménez-Castro Mónica B. , Cornide-Petronio María Eugenia , Gracia-Sancho Jordi , Casillas-Ramírez Araní , Peralta Carmen
Published: April 2019

We analyzed the participation of mitogen-activated protein kinases (MAPKs), namely p38, JNK and ERK 1/2 in steatotic and non-steatotic livers undergoing ischemia-reperfusion (I-R), an unresolved problem in clinical practice. Hepatic steatosis is a major risk factor in liver surgery because these types of liver tolerate poorly to I-R injury. Also, a further increase in the prevalence of steatosis in liver surgery is to be expected. The possible therapies based on MAPK regulation aimed at reducing hepatic I-R injury will be discussed. Moreover, we reviewed the relevance of MAPK in ischemic preconditioning (PC) and evaluated whether MAPK regulators could mimic its benefits. Clinical studies indicated that this surgical strategy could be appropriate for liver surgery in both steatotic and non-steatotic livers undergoing I-R. The data presented herein suggest that further investigations are required to elucidate more extensively the mechanisms by which these kinases work in hepatic I-R. Also, further researchers based in the development of drugs that regulate MAPKs selectively are required before such approaches can be translated into clinical liver surgery.
Newborn Screening for Selected Disorders in Nepal: A Pilot Study
Authors: Sharma Pandey Arti , Joshi Suchita , Rajbhandari Rateena , Kansakar Prerana , Dhakal Sadichhya , Fingerhut Ralph
Published: April 2019

The prevalence of metabolic disorders in Nepal is yet unknown, although many case reports occur in literature. Heel-prick blood samples from newborns were collected on Dried Blood Spot (DBS) collection cards and tested through Tandem Mass Spectroscopy and fluorescence assays for disorders included in the Swiss neonatal screening program; two cases of hypothyroidism and one case of cystic fibrosis were identified. Thyroid stimulating hormone (TSH), immuoreactive trypsinogen (IRT), hydroxyprogesterone (OHP), tyrosine (Tyr), and octanoylcarnitine (C8) showed significant differences with gestation age. Most of the parameters were positively correlated with each other except galactose, galactose 1 phosphate uridyl transferase (GALT), and biotinidase. First and ninety-ninth percentiles in the Nepalese newborns were found to be different when compared with the Swiss newborns. Congenital hypothyroidism and cystic fibrosis are candidates to be considered for a newborn screening program in Nepal. Differences between the Nepalese and Swiss newborns in parametric values that change with gestation age can be attributed to a higher survival rate of pre-term babies in Switzerland. Others could be explained in part by early and exclusive breastfeeding in Nepalese newborns.
Game Analysis of Access Control Based on User Behavior Trust
Authors: Wang Yan , Tian Liqin , Chen Zhenguo
Published: April 2019

Due to the dynamics and uncertainty of the current network environment, access control is one of the most important factors in guaranteeing network information security. How to construct a scientific and accurate access control model is a current research focus. In actual access control mechanisms, users with high trust values bring better benefits, but the losses will also be greater once cheating access is adopted. A general access control game model that can reflect both trust and risk is established in this paper. First, we construct an access control game model with user behavior trust between the user and the service provider, in which the benefits and losses are quantified by using adaptive regulatory factors and the user’s trust level, which enhances the rationality of the policy making. Meanwhile, we present two kinds of solutions for the prisoner’s dilemma in the traditional access control game model without user behavior trust. Then, due to the vulnerability of trust, the user’s trust value is updated according to the interaction situation in the previous stage, which ensures that the updating of the user’s trust value can satisfy the “slow rising-fast falling” principle. Theoretical analysis and the simulation experiment both show that this model has a better performance than a traditional game model and can guarantee scientific decision-making in the access control mechanism.
Cardiac Glycosides in Human Physiology and Disease: Update for Entomologists
Authors: El-Mallakh Rif S. , Brar Kanwarjeet S. , Yeruva Rajashekar Reddy
Published: April 2019

Cardiac glycosides, cardenolides and bufadienolides, are elaborated by several plant or animal species to prevent grazing or predation. Entomologists have characterized several insect species that have evolved the ability to sequester these glycosides in their tissues to reduce their palatability and, thus, reduce predation. Cardiac glycosides are known to interact with the sodium- and potassium-activated adenosine triphosphatase, or sodium pump, through a specific receptor-binding site. Over the last couple of decades, and since entomologic studies, it has become clear that mammals synthesize endogenous cardenolides that closely resemble or are identical to compounds of plant origin and those sequestered by insects. The most important of these are ouabain-like compounds. These compounds are essential for the regulation of normal ionic physiology in mammals. Importantly, at physiologic picomolar or nanomolar concentrations, endogenous ouabain, a cardenolide, stimulates the sodium pump, activates second messengers, and may even function as a growth factor. This is in contrast to the pharmacologic or toxic micromolar or milimolar concentrations achieved after consumption of exogenous cardenolides (by consuming medications, plants, or insects), which inhibit the pump and result in either a desired medical outcome, or the toxic consequence of sodium pump inhibition.
GLP-1 Limits Adipocyte Inflammation and Its Low Circulating Pre-Operative Concentrations Predict Worse Type 2 Diabetes Remission after Bariatric Surgery in Obese Patients
Authors: Izaguirre Maitane , Gómez-Ambrosi Javier , Rodríguez Amaia , Ramírez Beatriz , Becerril Sara , Valentí Víctor , Moncada Rafael , Unamuno Xabier , Silva Camilo , de la Higuera Magdalena , Salvador Javier , Monreal Ignacio , Frühbeck Gema , Catalán Victoria
Published: April 2019

Objective: Glucagon-like peptide (GLP)-1 has been proposed as a key candidate in glucose improvements after bariatric surgery. Our aim was to explore the role of GLP-1 in surgically-induced type 2 diabetes (T2D) improvement and its capacity to regulate human adipocyte inflammation. Methods: Basal circulating concentrations of GLP-1 as well as during an oral glucose tolerance test (OGTT) were measured in lean and obese volunteers with and without T2D (n = 93). In addition, GLP-1 levels were determined before and after weight loss achieved by Roux-en-Y gastric bypass (RYGB) (n = 77). The impact of GLP-1 on inflammation signalling pathways was also evaluated. Results: We show that the reduced (p < 0.05) circulating levels of GLP-1 in obese T2D patients increased (p < 0.05) after RYGB. The area under the curve was significantly lower in obese patients with (p < 0.01) and without (p < 0.05) T2D compared to lean volunteers while obese patients with T2D exhibited decreased GLP-1 levels at baseline (p < 0.05) and 120 min (p < 0.01) after the OGTT. Importantly, higher (p < 0.05) pre-operative GLP-1 concentrations were found in patients with T2D remission after RYGB. We also revealed that exendin-4, a GLP-1 agonist, downregulated the expression of inflammation-related genes (IL1B, IL6, IL8, TNF) and, conversely, upregulated the mRNA levels of ADIPOQ in human visceral adipocytes. Furthermore, exendin-4 blocked (p < 0.05) LPS-induced inflammation in human adipocytes via downregulating the expression and secretion of key inflammatory markers. Conclusions: Our data indicate that GLP-1 may contribute to glycemic control and exert a role in T2D remission after RYGB. GLP-1 is also involved in limiting inflammation in human visceral adipocytes.
Early-Life Exposure to the Chinese Famine and Risk of Cognitive Decline
Authors: Rong Hongguo , Lai Xiaozhen , Mahmoudi Elham , Fang Hai
Published: April 2019

Previous studies on the Chinese famine suggested long-term effects of early-life famine exposure on health conditions. This study aims to investigate the association between exposure to the Chinese famine of 1959–1961 at different early-life stages and the risk of cognitive decline in adulthood. A total of 6417 adults born between 1952 and 1964 in the 2015 survey data of China Health and Retirement Longitudinal Study were included in this study. Cognitive performance was estimated through a series of comprehensive neuropsychological tests, including the Telephone Interview of Cognitive Status (TICS-10), word recall, and pentagon drawing. Multiple generalized linear model (GLM) was employed to detect the association between multi-stage early-life famine exposure and late-life cognitive performance. Compared with the unexposed group, respondents exposed to famine in the fetal period performed worse in the TICS (difference −0.52, 95% confidence interval (CI): −0.93 to −0.10), word recall (difference −0.46, 95% CI: −0.74 to −0.19), and general cognition (difference −1.05, 95% CI: −1.64 to −0.47). Furthermore, we also found negative effects of famine exposure on performance of word recall and pentagon drawing in the early (word recall difference −0.56, 95% CI: −1.00 to −0.11; pentagon drawing difference −0.76, 95% CI: −1.40 to −0.12), mid (word recall difference −0.46, 95% CI: −0.81 to −0.11; pentagon drawing difference −0.66, 95% CI: −1.16 to −0.16), and late (word recall difference −0.30, 95% CI: −0.55 to −0.04; pentagon drawing difference −0.75, 95% CI: −1.13 to −0.37) childhood-exposed groups. Early-life famine exposure in different stages is positively associated with late-life cognitive decline. Fetal famine exposure might affect the overall cognitive status in adulthood, and childhood famine exposure has potential adverse effects on visuospatial episodic memory.
Efficient Numerical Pricing of American Call Options Using Symmetry Arguments
Authors: Stentoft Lars
Published: April 2019

This paper demonstrates that it is possible to improve significantly on the estimated call prices obtained with the regression and simulation-based least-squares Monte Carlo method by using put-call symmetry. The results show that, for a large sample of options with characteristics of relevance in real-life applications, the symmetric method performs much better on average than the regular pricing method, is the best method for most of the options, never performs poorly and, as a result, is extremely efficient compared to the optimal, but unfeasible method that picks the method with the smallest Root Mean Squared Error (RMSE). A simple classification method is proposed that, by optimally selecting among estimates from the symmetric method with a reasonably small order used in the polynomial approximation, achieves a relative efficiency of more than 98 % . The relative importance of using the symmetric method increases with option maturity and with asset volatility. Using the symmetric method to price, for example, real options, many of which are call options with long maturities on volatile assets, for example energy, could therefore improve the estimates significantly by decreasing their bias and RMSE by orders of magnitude.
What They Did Not Tell You about Algebraic (Non-) Existence, Mathematical (IR-)Regularity, and (Non-) Asymptotic Properties of the Dynamic Conditional Correlation (DCC) Model
Authors: McAleer Michael
Published: April 2019

In order to hedge efficiently, persistently high negative covariances or, equivalently, correlations, between risky assets and the hedging instruments are intended to mitigate against financial risk and subsequent losses. If there is more than one hedging instrument, multivariate covariances and correlations have to be calculated. As optimal hedge ratios are unlikely to remain constant using high frequency data, it is essential to specify dynamic time-varying models of covariances and correlations. These values can either be determined analytically or numerically on the basis of highly advanced computer simulations. Analytical developments are occasionally promulgated for multivariate conditional volatility models. The primary purpose of this paper is to analyze purported analytical developments for the only multivariate dynamic conditional correlation model to have been developed to date, namely the widely used Dynamic Conditional Correlation (DCC) model. Dynamic models are not straightforward (or even possible) to translate in terms of the algebraic existence, underlying stochastic processes, specification, mathematical regularity conditions, and asymptotic properties of consistency and asymptotic normality, or the lack thereof. This paper presents a critical analysis, discussion, evaluation, and presentation of caveats relating to the DCC model, with an emphasis on the numerous dos and don’ts in implementing the DCC model, as well as a related model, in practice.
State-Owned Entities as Key Actors in the Promotion and Implementation of the 2030 Agenda for Sustainable Development: Examples of Good Practices
Authors: Barnes Mihaela-Maria
Published: April 2019

The purpose of this article is to demonstrate that a wide range of entities associated with the State and which engage in business or investment activities on behalf of the State have an important role to play in the promotion and implementation of the sustainable development goals found in the 2030 Agenda. The contribution starts with a background to the 2030 Agenda, followed by an introduction to the features of State-owned entities. Since the 2030 Agenda requires the ‘mobilization of all available resources’ to achieve its implementation, it is argued that entities that are owned by States could have a significant role to play in this context. Examples of good practices from a number of jurisdictions show how the development and implementation of domestic measures that cover State-owned entities have the potential to contribute to the promotion and implementation of the 2030 Agenda.
Effect of LiNO3 on Expansion of Alkali–Silica Reaction in Rock Prisms and Concrete Microbars Prepared by Sandstone
Authors: Liu Jinxin , Yu Lanqing , Deng Min
Published: April 2019

The aim of this research is to investigate the effect of LiNO3 on the alkali–silica reaction (ASR) expansion of reactive sandstone and the mechanism through which this occurs. This paper presents the results from tests carried out on rock prisms and concrete microbars prepared by sandstone and LiNO3. The findings show that LiNO3 does not decrease the expansion of these samples unless the molar ratio of [Li]/[Na + K] exceeds 1.66, and the expansion is greatly increased when its concentration is below this critical concentration. The expansion stress test proves that Li2SiO3 is obviously expansive. X-ray diffraction (XRD) and scanning electron microscope (SEM) results indicate that LiNO3 reacts with the microcrystalline quartz inside sandstone, inhibiting the formation of ASR gel, and the formation of Li2SiO3 causes larger expansion. A high concentration of LiNO3 might inhibit the ASR reaction in the early stages, and the formation of Li2SiO3 causes expansion and cracks in concrete after a long period of time.
Some of the Latest Active Strengthening Techniques for Masonry Buildings: A Critical Analysis
Authors: Ferretti Elena , Pascale Giovanni
Published: April 2019

The present paper deals with the retrofitting of unreinforced masonry (URM) buildings, subjected to in-plane shear and out of-plane loading when struck by an earthquake. After an introductive comparison between some of the latest punctual and continuous active retrofitting methods, the authors focused on the two most effective active continuous techniques, the CAM (Active Confinement of Masonry) system and the Φ system, which also improve the box-type behavior of buildings. These two retrofitting systems allow increasing both the static and dynamic load-bearing capacity of masonry buildings. Nevertheless, information on how they actually modify the stress field in static conditions is lacking and sometimes questionable in the literature. Therefore, the authors performed a static analysis in the plane of Mohr/Coulomb, with the dual intent to clarify which of the two is preferable under static conditions and whether the models currently used to design the retrofitting systems are fully adequate.
GHOST—Gate to Hybrid Optimization of Structural Topologies
Authors: Bochenek Bogdan , Tajs-Zielińska Katarzyna
Published: April 2019

Although well-recognized in the fields of structural and material design and widely present in engineering literature, topology optimization still arouses a high interest within research communities. Moreover, it is observed that the development of innovative, efficient and versatile methods is one of the most important issues stimulating progress within the topology optimization area. Following this activity, in the present study, a concept of a hybrid algorithm developed in order to generate optimal structural topologies of minimal compliance is presented. The hybrid algorithm is built based on two existing approaches. The first one makes use of the formal optimality criterion, whereas the second one utilizes a special heuristic rule of design variables updating. The main idea that stands behind the concept of the present proposal is to take the advantage of both algorithms capabilities. In a numerical implementation of the hybrid algorithm, the design variables are updated at each iteration step using both approaches, and the solution with a lower objective function value is selected for the next iteration. The numerical tests of the generation of minimal compliance structures have been performed for chosen structures including a real engineering one. It has been confirmed that the proposed hybrid technique based on switching between the considered rules allows the final structures having lower values of compliance as compared with the results of an application of basic algorithms running separately to be obtained. Moreover, based on promising results of the tests performed, one can consider the proposed concept of a hybrid algorithm as an alternative for other existing topology generators.