bluuebunny/crossref_metadata_2025 huggingface dataset:
Geodatabase and Health Risk Assessment of Avenue Trees within Three Selected Roads in the University of Ibadan, Ibadan, Nigeria
Authors: Israel Rebecca , Alo Akintunde
Published: July 2023

The campuses of Nigerian universities especially the old ones, like the University of Ibadan, comprise old trees which can pose a threat to pedestrians, structures, and roads within the school environments. However, there is no adequate information on these avenue trees thereby impeding their sustainable management. Therefore, this study provides the basic information needed by developing a geodatabase for the avenue trees along Benue, Emotan and Oduduwa roads within the University of Ibadan, Ibadan, Nigeria. The coordinates of all the avenue trees, and some within the selected roads were obtained. Tree growth data collected include; diameter at the breast height (dbh), diameter at the base, middle and at the top, crown height, crown diameter, and total height of all the avenue trees. Tree visual evaluation for diseases (TVED) was carried out for root decay, and termite attack on any part of the tree (stem, leaves and visible part of the root). Risk rate was assessed as low, medium and high, depending on the potential target (human, vehicles, structures). The risk rate depended on the incidence of leaning towards a building or road, cracked branches, root attack by termites, basal decay or branch decay. The growth data were used to compute the tree volume while the avenue trees were georeferenced. The physical health status of the avenue trees was determined using TVED data. The spatial distribution of the avenue trees was developed in GIS while all the analysed data were pulled together to develop a geodatabase for the avenue tress using design templates of BOOTSTRAP, comprising of Hypertext Markup Language, Cascade Style Sheets, JavaScripts and Hypertext Preprocessor. A total of 121 individual avenue trees belonging to 14 species were identified in the study area. The analysis of the geospatial distribution shows that Emotan Road had a lesser spatial distribution of avenue trees compared to Benue and Oduduwa roads. Furthermore, the health risk assessment of the trees in the study area indicated that 17.35% of the individual avenue trees had defects. The database created is user-friendly, suitable and has the ability for easy data storage and quick information retrieval on the avenue trees to enhance their maintenance and risk management. This can be accessed using the link http://159.203.29.155/rebs/rebs.php.
CRISPR/Cas Technology Revolutionizes Crop Breeding
Authors: Tang Qiaoling , Wang Xujing , Jin Xi , Zhang Haiwen , Wang Youhua
Published: July 2023

Crop breeding is an important global strategy to meet sustainable food demand. CRISPR-Cas is a most promising gene-editing technology for rapid and precise generation of novel germplasm, and leads to revolutionary crop breeding innovation. In this review, we summarize recent advance of CRISPR/Cas technology in gene function analyses and generation of new germplasms with increased yield, improved product quality, and enhanced resistance to biotic and abiotic stress. We highlight their applications and breakthroughs in agriculture including crop de novo domestication, decoupling the gene pleiotropy tradeoff, crop hybrid seed conventional production, hybrid rice asexual reproduction, and double haploid breeding. Moreover, the challenges and development of CRISPR/Cas technology in crops are also discussed.
Lateral Force and EMG Activity in Wide and Narrow Grip Bench Press in Various Conditions
Authors: Tanimoto Michiya , Arakawa Hiroshi , Sato Mauto , Nagano Akinori
Published: July 2023

The purpose of this study was to investigate the horizontal force and contribution of shoulder horizontal adductor and elbow extensor muscles activity during wide and narrow grip bench press (BP) in various conditions, such as resistance trained/non-trained, concentric/eccentric and muscle fatigue/non-fatigue.　We measured the horizontal force on the bar and the electromyographic (EMG) muscle activity of pectoralis major (PM) and triceps brachii (TB) during 10RM BP with wide grip (81cm) and narrow grip (40cm)　in 7　resistance trained men and 7 non-trained men. The all-reps average of the horizontal to vertical force ratio both in resistance trained and non-trained subjects was about 30% outward for the wide grip, and about 10% inward for the narrow grip. The EMG contribution ratio PM/TB shows no significant differences between narrow and wide grip in all evaluated conditions except in non-trained subjects muscle fatigue eccentric phase. Both resistance trained and non-trained subjects did not push the bar straight upward, and the EMG PM/TB was almost unchanged by hand width. The direction adjustment of the force on the bar that achieves almost the same muscle activity degree of the shoulder and elbow joints might be optimal BP kinetics.
Exploring Model Structures to Reduce Data Requirements for Neural ODE Learning in Control Systems
Authors: Hashimoto Takanori , Matsui Nobuyuki , Kamiura Naotake , Isokawa Teijiro
Published: July 2023

In this study, we investigate model structures for neural ODEs to improve the data efficiency in learning the dynamics of control systems. We introduce two model structures and compare them with a typical baseline structure. The first structure considers the relationship between the coordinates and velocities of the control system, while the second structure adds linearity with respect to the control term to the first structure. Both of these structures can be easily implemented without requiring additional computation. In numerical experiments, we evaluate these structure on simulated simple pendulum and CartPole systems and show that incorporating these characteristics into the model structure leads to accurate learning with a smaller amount of training data compared to the baseline structure.
Lightweight Bilateral Network for Real-Time Semantic Segmentation
Authors: Wang Pengtao , Li Lihong , Pan Feiyang , Wang Lin
Published: July 2023

Herein, a dual-branch semantic segmentation model based on depth-separable convolution and attention mechanism is proposed for the real-time and accuracy requirement of semantic segmentation. The proposed approach overcomes the problems of poor segmentation effect and over-simplification of feature fusion arising from the constant downsample operations in semantic segmentation. The network is divided into spatial detail and semantic information paths. The spatial detail path utilizes a smaller downsample multiplier to maintain resolution and efficiently extract spatial information. The semantic information path is constructed by a non-bottleneck residual unit with dilated convolution; it extracts semantic features. For the feature aggregation problem, the feature-guided fusion module is designed to assign different weights to the parts of the two paths and fuse them to obtain the final output. The proposed algorithm achieves a segmentation accuracy of 69.6% and speed of 70 fps on the Cityscapes dataset, with a model parameter count of only 0.76 M, thus indicating some advantages over recent real-time semantic segmentation algorithms. The proposed method with depth separable convolution and attention mechanism can effectively extract features and compensate for the loss of accuracy caused by downsampling. The experiments demonstrate that the proposed fusion module outperforms other methods in fusing different features.
Surgical Site Infection Is Not Associated with 1-Year Progression-Free Survival After Endoprosthetic Reconstruction for Lower-Extremity Osteosarcoma
Authors: Jackson Kristopher J. , Sullivan Camille D. , Zimel Melissa N. , Wustrack Rosanna L. ,   on behalf of the PARITY Investigators
Published: July 2023

Background:

Although there is evidence suggesting that postoperative infection confers a survival benefit in osteosarcoma treated with resection and endoprosthetic reconstruction, there have been no prospective studies to date to support these findings. This secondary analysis of Prophylactic Antibiotic Regimens in Tumor Surgery (PARITY) study data examines the relationship between surgical site infection (SSI) and disease progression within 12 months after limb salvage surgery.

Methods:

The PARITY trial was an international, multicenter, prospective randomized controlled trial of 604 patients who underwent resection of a lower-extremity bone tumor and endoprosthetic reconstruction. Our primary outcome was progression-free survival (PFS) at 1 year following surgery among the patients with osteosarcoma. Subgroup analyses by disease stage at presentation and infection severity were also performed. Cox proportional hazard models were employed to examine the association between clinical and tumor characteristics, SSI, and PFS. Kaplan-Meier analysis was used to determine the effect of SSI on PFS.

Results:

The 274 PARITY patients with osteosarcoma were included in this secondary analysis. Thirty-two (11.7%) of the patients presented with metastasis at baseline; 53 (19.3%) of the patients developed an SSI. There was no difference in 1-year PFS between patients with and without SSI. There was no decreased risk of disease progression at 1 year in patients with localized disease at baseline who developed an SSI (hazard ratio [HR] = 1.21; 95% confidence interval [CI] = 0.64 to 2.28). Infection was associated with increased disease progression at 1 year in patients with baseline metastases (HR = 4.26; 95% CI = 1.11 to 16.3).

Conclusions:

No positive association was detected between postoperative infection and PFS at 1 year following surgery in this secondary analysis of prospective data. However, this analysis suggests infection could be a risk factor for early disease progression in patients with baseline metastases, and future investigations may better elucidate the association between disease burden and the host immune response to advance immunotherapeutic strategies for osteosarcoma.

Level of Evidence:

Prognostic Level II. See Instructions for Authors for a complete description of levels of evidence.
Open Versus Core Needle Biopsy in Lower-Extremity Sarcoma
Authors: Burke Zachary D.C. , Lazarides Alexander L. , Gundavda Manit K. , Griffin Anthony M. , Tsoi Kim M. , Ferguson Peter C. , Wunder Jay S. ,   on behalf of the PARITY Investigators
Published: July 2023

Background:

Historically, open biopsy (OB) was the gold standard for sarcoma diagnosis. Core needle biopsy (CNB) has become increasingly common. There are limited data evaluating how the type of biopsy impacts definitive surgical resection or postoperative outcomes. The aims of this study were to (1) characterize current international biopsy practice patterns, and (2) evaluate how the type of biopsy performed impacts the resection surgery, infection risk, oncological complications, and patient-reported functional outcome scores.

Methods:

This study was a preplanned secondary analysis of the prospective, multicenter PARITY (Prophylactic Antibiotic Regimens in Tumor Surgery) study. Patients with a benign diagnosis, metastatic disease, or no biopsy prior to surgery were excluded. Prospectively collected demographic, biopsy, surgical, and outcome variables were analyzed, and differences between patients undergoing OB and CNB were assessed. Parametric and nonparametric tests were used to compare variables between groups, and the Cox proportional hazards method was used to compare infection-related and oncological outcomes at 1 year. Median functional outcome scores at 1 year were compared.

Results:

Four hundred and sixty-four patients met the inclusion criteria. Data were collected from 48 sarcoma centers in 12 countries. CNB was the more utilized biopsy modality overall (57.5%). OB was more common in the U.S. and Canada. The median operative time was significantly longer for patients who underwent OB (324 versus 260 minutes; p < 0.001). Significantly more skin (p < 0.001) and fascial tissue (p < 0.001) were excised in the OB group, which also had a lower rate of primary closure (86.3% versus 92.9%; p = 0.03). There were no differences in surgical site infection or oncological outcomes between the groups at 1-year follow-up.

Conclusions:

CNB was the more common biopsy modality in the PARITY study in most countries. However, OB was more common in the U.S. and Canada. Patients undergoing OB had longer operative times, more excised tissue, and lower rates of primary closure, but this did not translate to differences in infection rates or oncological outcomes, including local recurrence.

Level of Evidence:

Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Locating and ranking development plans of Iron and Steel Industries in Afghanistan by using FAHP and FTOPSIS methods
Authors: salehy Saleh Mohammad , Aimaq Mohammad Bashir , Shahabi Reza Shakoor
Published: July 2023

Due to the growth of steel industry in the world, the rich resources of ore, Abundance of inputs required by the iron and steel industry and higher demand for steel in Afghanistan, the idea of ​​designing and locating development plans in the field of iron and steel industry was created. To increase existing competitive advantages and gaining the most added value for Afghanistan requires suitable locations. In this paper, six cases as criteria and nineteen cases as sub-criteria that have a greater impact on the construction of iron and steel industries, selected after interviews with experts. The questionnaires have been arranged and distributed by google-forms and interviews have been conducted with experts. The weights of the criteria and sub-criteria were calculated based on the results of the questionnaires and using the FAHP. Then by using the FTOPSIS, the plans are ranked based on the similarity index. As a result, which the Bamyan has come in first position and Kandahar has come in recent position of development planning to invest. In the end, for more accuracy and correctness of the research after completing all preconditions, sensibility analysis among options according criteria and sub-criteria has been done in five scenarios.
Antibiotic Resistance of Diverse MLST Campylobacter from Pediatric Patients with Acute Diarrhea
Authors: Hou Shuiping , Mei Peng , Shi Runfeng , Chen Kuncai , pan Xinhong , Chen shouyi , Li Juntao
Published: July 2023

Background
Campylobacter spp., as a zoonotic pathogen, has been one of the most common pathogens that causes human gastroenteritis in developing countries. The objective of this study was to determine the antibiotic resistance of Campylobacter in children.
Methods
Stool samples were collected from pediatric patients under 14 years of age who visited hospital for acute diarrhea between February 2018 and January 2019. Campylobacter was isolated from samples using selective enrichment and culture and confirmed by PCR, genotypes were characterized by multilocus sequencing, and antibiotic resistance was determined by susceptibility and resistance genes to antimicrobials of medical importance.
Results
The overall prevalence of Campylobacter in children with diarrhea was 4.4% (53/1197). Higher prevalence was observed in children under five-year-old and in spring and autumn. Approximately 80% (42/53) of confirmed isolates were C. jejuni and ~ 20% (11/53) isolates were C. coli. Among the 51 isolates successfully typed, 43 sequence types belonging to 13 clonal complexes were identified, of which 44.2% (19/43) sequence types were novel. The majority of the Campylobacter isolates (74%) exhibited multidrug resistance and the most common antimicrobials resistant to were ciprofloxacin, tetracycline and nalidixic acid. All isolates (53/53) were positive of the tet(O) gene that confers resistance to tetracycline and the gyrA gene that confers resistance to quinolones/fluoroquinolones, 81% (43/53) isolates were positive of the ermB gene that confers resistance to macrolides, and 19% (10/53) isolates were positive of the aadE-sat4-aphA gene that confers resistance to aminoglycosides, respectively.
Conclusions
Despite the low prevalence of Campylobacter in children in the subtropical Guangzhou metropolitan, the high multidrug resistance of diverse MLST of Campylobacter indicated the importance of preventive strategies including routine surveillance in order to minimize the infections of antibiotic resistant Campylobacter in children.
Physical Activity and Health-Related Quality of Life in Older Adults: Depression as a Mediator
Authors: Li Xiuxiu , Wang Pengfei , Jiang Yihua , Yang Yinghua , Wang Feng , Yan Fei , Peng Wenjia , Wang Ying
Published: July 2023

Background
Physical activity is associated with health-related quality of life (HRQoL) among older adults, and both are associated with mood, such as depression. However, the indirect effects of physical activity on HRQoL in older adults have not been clearly established. This study explains how physical activity is associated with HRQoL while considering the effects of depression in older adults.
Methods
A cross-sectional study was conducted with 7,518 community-dwelling older adults aged 60 years and older. Physical activity, depression, and HRQOL were measured using the Physical Activity Scale for the Elderly, the 30-item Geriatric Depression Scale, and the 36-Item Short-Form Health Survey (SF-36), respectively. Physical activity included three types of activities: leisure, household, and work. Information on age, gender, education, monthly income, physical functioning, smoking, and alcohol use were also collected.
Results
The multivariate analyses showed that after adjusting for sociodemographic variables, SF-36 Physical Component Summary scores were negatively associated with depression (B = -2.046, 95% CI [2.584, -1.509]) and positively with physical activity (p < 0.001). Similarly, SF-36 Mental Component Summary scores were negatively associated with depression (B = -11.657, 95% CI [-12.190, -11.124]). In the mediation analysis, different types and levels of physical activity positively correlated with HRQoL (p < 0.001), and depression negatively correlated with physical activity and HRQoL (p < 0.001).
Conclusions
The findings showed that lighter amounts of physical activity and worsening HRQoL in Chinese older adults were independently associated. In addition, the relationship between physical activity and HRQoL was partially mediated by depression. It is recommended that geriatric health care administrators and health care planners prioritize improving physical activity levels in older adults to promote the reduction of depressive symptoms in older adults to improve the beneficial effects on HRQoL.
The ENCODE Uniform Analysis Pipelines
Authors: Hitz Benjamin , Lee Jin-Wook , Jolanki Otto , Kagda Meenakshi , Graham Keenan , Sud Paul , Gabdank Idan , Strattan J. Seth , Sloan Cricket , Dreszer Timothy , Rowe Laurence , Podduturi Nikhil , Malladi Venkat , Chan Esther , Davidson Jean , Ho Marcus , Miyasato Stuart , Simison Matt , Tanaka Forrest , Luo Yunhai , Wahling Ian , Lin Khine Zin , Jou Jennifer , Hong Eurie , Lee Brian , Sandstrom Richard , Rynes Eric , Nelson Jemma , Nishida Andrew , Ingersoll Alyssa , Buckley Michael , Frerker Mark , Kim Daniel , Boley Nathan , Trout Diane , Dobin Alexander , Rahmanian Sorena , Wyman Dana , Balderrama-Gutierrez Gabriela , Reese Fairlie , Durand Neva , Dudchenko Olga , Weisz David , Rao Suhas , Blackburn Alyssa , Gkountaroulis Dimos , Sadr Mahdi , Olshansky Moshe , Eliaz Yossi , Nguyen Dat , Bochkov Ivan , Shamim Muhammad , Mahajan Ragini , Aiden Erez , Gingeras Thomas , Heath Simon , Hirst Martin , Kent W. James , Kundaje Anshul , Mortazavi Ali , Wold Barbara , Cherry J.
Published: July 2023

The Encyclopedia of DNA elements (ENCODE) project is a collaborative effort to create a comprehensive catalog of functional elements in the human genome. The current database comprises more than 19000 functional genomics experiments across more than 1000 cell lines and tissues using a wide array of experimental techniques to study the chromatin structure, regulatory and transcriptional landscape of the Homo sapiens and Mus musculus genomes. All experimental data, metadata, and associated computational analyses created by the ENCODE consortium are submitted to the Data Coordination Center (DCC) for validation, tracking, storage, and distribution to community resources and the scientific community. The ENCODE project has engineered and distributed uniform processing pipelines in order to promote data provenance and reproducibility as well as allow interoperability between genomic resources and other consortia. All data files, reference genome versions, software versions, and parameters used by the pipelines are captured and available via the ENCODE Portal. The pipeline code, developed using Docker and Workflow Description Language (WDL; https://openwdl.org/) is publicly available in GitHub, with images available on Dockerhub (https://hub.docker.com), enabling access to a diverse range of biomedical researchers. ENCODE pipelines maintained and used by the DCC can be installed to run on personal computers, local HPC clusters, or in cloud computing environments via Cromwell. Access to the pipelines and data via the cloud allows small labs the ability to use the data or software without access to institutional compute clusters. Standardization of the computational methodologies for analysis and quality control leads to comparable results from different ENCODE collections - a prerequisite for successful integrative analyses.
Intelligent vector-based path following guidance law for unmanned surface vehicles
Authors: ÜNAL Osman , AKKAŞ Nuri , ATALI Gökhan
Published: July 2023

The authors have requested that this preprint be removed from Research Square.
Prevalence and Associated Risk Factors of Resistant Hypertension among Chinese Hypertensive Patients in Primary Care Setting
Authors: Chan Kilpatrick Kiupak , Chiang Lapkin , Choi Clarence Chuenming , Li Yimchu , Chen Catherine Xiarui
Published: July 2023

Introduction:
Hypertension (HT) is a major public health problem globally, and it is the commonest chronic disease with a prevalence of 27% among people aged 15 years or above in Hong Kong. There is emerging literature confirmed that patients with resistant hypertension (RHT) give its increased risk for adverse clinical outcomes and higher rate of documented target organ damage. This study aims to identifythe prevalence of RHT among Chinese hypertensive patients managed in public primary care setting of Hong Kong and exploring its associated risk factors.
Methodology: This is a cross-sectional descriptive study. Chinese hypertensive patients aged 30 or above with regular follow-up between 1st July 2019 and 30th June 2020 in 10 public primary care clinics under the Hospital Authority of Hong Kong were included. Demographic data, clinical parameters and drug profile of patients were retrieved from its computerized record system. The prevalence of RHT was identified and the associated risk factors of RHT were explored by multivariate logistic regression analysis.
Results: Among the 538 sampled Chinese hypertensive patients, the mean age was 67.4 ±11.5 years old, and 51.9% were female. The mean duration of hypertension was 10.1 ± 6.4 years, with a mean systolic and diastolic blood pressure of 128 ± 12.3 and 74 ± 10.8 mmHg respectively. 40 out of 538 patients were found to have RHT, giving an overall prevalence of 7.43%. Four factors were found to be associated with increased risk of RHT, in ascending order of odds ratio: duration of hypertension (OR 1.08), male gender (OR 2.72), comorbid with type 2 diabetes mellitus (T2DM, OR 2.99), and congestive heart failure (CHF, OR 5.39).
Conclusion: The prevalence of RHT among Chinese hypertensive patients in primary care setting of Hong Kong is 7.43%. RHT is more common in male patients, patients with longer duration of hypertension, concomitant T2DM and CHF. Clinicians should be vigilant when managing these groups of patients and provide aggressive treatment and close monitoring.
Whitlockite nanoparticle-coated hydroxyapatite granules for bone regeneration in defect of rat calvaria
Authors: Lee Won-Bum , Wang Caifeng , Jang Yoon-Seo , Jeong Ki-Jae , Kang Hyojin , Seol Ayun , Park Eun Seo , Lee Ho-Jin , Hwang Dae Youn , Lee Jaebeom , Hwang Dae-Seok
Published: July 2023

Background
Whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) as the second most abundant ceramic of human bone after hydroxyapatite (HA; Ca10(PO4)6(OH)2), possessing high osteogenic activity, has been known that it can stimulate osteogenic differentiation as well as suppress osteoclastic activity, particularly, in the preparatory stage of bone regeneration.
Methods
In this work, we construct a unique ceramic structure by layering WH on the surface of HA granules (HAGs)s via dip-coating and sintering method.
Results
The cell proliferation of the WH-coated HAGs (WHHAGs) group in the cell counting kit (CCK-8) was about 1.15-fold higher than that of the NT group at 72 h after incubation. The western blot and qPCR results demonstrated that WHHAGs treatment readily stimulated the transcription of osteogenic genes by regulating the downstream signaling pathway of BMP and WNT receptors.This study presents a histological and radiological evaluation of the bone healing potential of the WHHAGs in a 7 mm diameter calvarial bone defect in rats, compared with/without the BMP and non-treated (NT) groups at the 8 weeks after surgery. The bone volume fraction (bone volume/tissue volume) of the WHHAGs group was about 7.32- and 3.56- fold higher than that of the NT group with/without the BMP, respectively. Besides, histological evaluation confirmed that the WHHAGs with BMP promoted bone regeneration.
Conclusion
These results suggest that the WHHAGs present the remarkable potential for application in dental and orthopedic bone regeneration.
Effect Of Molnupiravir On Ichemia/Reperfusion Damage In Rat Liver, An Experimental Study
Authors: TAS Hakan Gokalp , TAS Necip Gokhan , KUYRUKLUYILDIZ Ufuk , ZIRH Selim , KURT Nezahat
Published: July 2023

Background
Ischemia occurs when blood supply to tissues is limited, resulting in cellular dysfunction and necrosis. Reperfusion, or the process of restoring blood flow, can result in an overabundance of reactive oxygen species (ROS) and toxic byproducts. The I/R (Ischemia / Reperfusion) technique used in liver resection and transplantation has been linked to liver damage. Molnupiravir, a non-hepatotoxic oral medicine, is converted into N-hydroxycytidine (NHC). The purpose of this study is to see how molnupiravir affects I/R-induced damage of liver in rats.
Methods
We divided the rats into three groups: Sham operation (SG) (n = 6), Liver I/R (LIR) (n = 6), and Molnupiravir + Liver I/R (MIR) (n = 6) groups. The MIR group (n = 6) received 40 mg/kg of molnupiravir. All animals were subjected to laparotomy, hepatic ischemia (1 hour), and reperfusion (6 hours). At the end of the trial, liver tissue samples were tested for IL-1β, tGSH, NF-κB, MDA, and TNF-α levels, as well as histopathological examination. The levels of ALT and AST in the blood were determined. The MIR group's results were in comparison to the SG and LIR groups.
Results
Biochemical examination revealed that NF-ƘB, MDA, TNF-α, IL-1β, ALT, and AST measurements were higher in the LIR and MIR groups than in the SG group. The SG group had the highest tGSH values. Histopathological examinations revealed that the MIR group had the most damage.
Conclusion
While molnupiravir, which is included in COVID-19 treatment regimen since it has no projected liver toxicity, does not affect healthy liver tissue, it does exacerbate ischemia/reperfusion injury in stressed liver tissue. Molnupiravir should be used with caution because it has the potential to aggravate liver damage during procedures such as liver transplantation or resection.
Preliminary study of HPV integration status on the occurrence and development of Vaginal intraepithelial neoplasia
Authors: Yuan Shuning , Qian Cheng , Zhang Hailong , Xing Yan
Published: July 2023

Vaginal intraepithelial neoplasm (VaIN), a precancerous lesion of vaginal cancer, increased distinctly in the past decade, while its occurrence after performing hysterectomy came to attention nowadays. As hr-HPV DNA integrates into host chromosome, transient HPV infection could transform into persistent infection to trigger high-grade intraepithelial neoplasm or HPV-related cancer. High-throughput Viral Integration Detection (HIVID) is a novel genetic sequencing method detecting HPV integration breakpoints for more precise sequence. There is no systematic study on the mechanism and risk assessment prediction system of VaIN in the vaginal stump after total hysterectomy for CIN. In this study, 24 cases suffered from VaIN were involved. HPV integration was detected in 11 cases, furthermore, we discovered HPV 16 and 73, chromosome 1 and 2 possessed most HPV integration sites while EMBP1, CLO5A1, EHF, ELF5 as dominate hot spots. Taken clinical outcome into account, we found a significant difference between HPV integration occurrence and VaIN(p = 0.011). HPV integration could serve as a predictive marker for monitoring and management of VaIN in a way.
Non-stationary associations between rainfall intensity and large-scale variability in an east-Andean city over the last 108-years
Authors: Medina Franco D. , Zossi Bruno S. , Elias Ana G.
Published: July 2023

This work explores for the 1912–2019 period the interannual associations between the maximum daily rainfall (Rx1) in San Miguel de Tucumán (Argentina, 26.8°S, 65.2°W) and the large-scale variability. The flood hazard in this east-Andean city is high due to the presence of lowlands and urban stream channels with poor maintenance. We analyzed the interannual relation between Rx1 and El Niño Southern Oscillation (ENSO) and the Southern Annular mode (SAM), and the non-stationarity of these associations as a response to Pacific Decadal Oscillation (PDO) and Atlantic Multidecadal Oscillation (AMO). We compute the moving correlation for all possible window lengths between Rx1-ENSO and Rx1-SAM. As a new approach we based our analysis on the periods with most significant correlations (i.e., with minimum p-value). The aim was to obtain a period when there exists an association with maximum statistical confidence. Results suggest that Rx1 variability shows the highest statistical significance in its association with ENSO for the 1945–1974 period and with SAM for the 1974–2007 period. The overall non-stationary nature in the relations can be attributed to the phase shifts of PDO. Negative PDO and positive ENSO phases favor higher Rx1 values, while this also occurs with both positive PDO and SAM. Inverse phases favor lower Rx1 values. On the other hand, AMO phases do not influence relationships. Results highlight that processes involved in the variability of rainfall intensity are partly related to global variability modes, opening opportunities to enhance predictions in relation to the flood hazard.
Stability Indicating Method Development and Validation for Anticoagulant Drug Apixaban in Apixaban Drug Substance and Drug Product
Authors: Suryawanshi Shriram Pandurang , Barabde Gayatri
Published: July 2023

The aimed of this research is to develop the method on reverse phase liquid chromatographic (RP-HPLC) for the assay determination of Apixaban in both drug substance and tablet dosage form. A Inertsil ODS C18 (5 μm particle size, 150 mm x 4.6 mm). was used for the RP-HPLC separation of Apixaban. The mobile phase consisted of a buffer solution pH 4 and methanol in a ratio of 50:50 v/v. The flow rate was set at 1.2 ml/minute, with an injection volume of 10 μL. The column oven temperature was maintained at 40°C, while the autosampler temperature was set to 25ºC. Detection of Apixaban was performed at a wavelength of 230 nm. The developed stability indicating analytical method underwent to validation in accordance with ICH guidelines.
Results: The chromatographic condition, results showed that the peak retention time of Apixaban was 2.2 min. The linearity of Apixaban was observed in the concentration range of 7.5 µg/mL to 37.5 µg/mL, with a percent accuracy was observed in ranging from 100.0% to 101.2%. Stress testing was conducted under acidic, basic, and oxidation conditions. The linearity correlation coefficient and system suitability criteria met the requirements specified by ICH guidelines.
Conclusions: The developed RP-HPLC method exhibited linearity and accuracy, with correlation coefficients and percent recovery falling within the acceptance criteria.The method proved to be simple, rapid, stability-indicating and cost-effective for the determination of Apixaban in both drug substance and tablet form.
QL-PPTS:A Machine Learning Scheduling Scheme on Edge Computing Environments
Authors: Lu Weifeng , Wan Jiahuan , Djigal Hamza , Xu Jia , Xu Lijie
Published: July 2023

Multiple Access Edge computing (MEC) is a computing model that can extend cloud computing and storage capabilities to the edge of cellular networks, close to IoT devices. MEC enables computation-constrained IoT devices to execute computation-intensive or low-latency applications at the edge of the network. However, in order to execute these applications efficiently, a reasonable scheduling strategy is required. Because of the particularity of edge environment, the traditional scheduling algorithm is not suitable. In this paper, we propose a new scheduling algorithm called QL-PPTS that combines reinforcement learning with a look-ahead list scheduling algorithm PPTS to reduce the makespan. we use QL to regard the rank of predictive cost matrix (rankP CM) of PPTS as the immediate reward. After the convergence of the Q-table, the priority queue of tasks is obtained according to the greedy principle. Experiments show that the QL-PPTS algorithm outperform the QL-HEFT.Specially, QL-PPTS can outperform QL-HEFT by 9% in terms of efficiency.
Long term outcome of Uniport versus Multiport Video-Assisted Thoracoscopic Lobectomy for Non-small Cell Lung Cancer: A Propensity-Matched Study
Authors: Ruan yingding , Cao Wenjun , Xue Hongsheng , You Maoduan , Zhao Zhilong
Published: July 2023

Background:Uniportal Video-Assisted Thoracoscopic Surgery (U-VATS) has emerged as a feasible approach for treating non-small cell lung cancer (NSCLC). However, there is a lack of studies reporting the long-term survival outcomes of U-VATS lobectomy for NSCLC.
Objective:This study aims to compare the perioperative outcomes and long-term survival of U-VATS lobectomy for NSCLC with multiportal VATS (M-VATS, involving two ports or more) lobectomy.
Method: A total of 339 patients who underwent intentional VATS lobectomy for NSCLC between 2012 and 2017 were included in the analysis. Perioperative outcomes and long-term survival were evaluated. Propensity score matching was utilized to minimize baseline characteristic differences between the two groups.
Results:Out of the total cases, 17 (5.01%) were converted to open thoracotomy. The conversion rates were 4.96% (7/141) in the U-VATS group and 5.05% (10/198) in the M-VATS group. A total of 322 consecutive patients underwent VATS lobectomy and mediastinal lymphadenectomy. After propensity matching, 106 pairs were obtained, consisting of 83 males and 129 females. Intraoperative bleeding volume, number of retrieved lymph nodes, explored nodal stations, drainage time and volume, and postoperative hospital stay were similar between the two groups. Both groups exhibited comparable morbidity and mortality rates. From the multivariable analysis, there was no significant difference observed in terms of overall survival (OS) and disease-free survival (DFS) between the two patient cohorts.
Conclusions: U-VATS demonstrated comparable perioperative outcomes and long-term efficacy to M-VATS. However, further confirmation of these findings is required
Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study
Authors: Yu Xin-xia , Song Si‑meng , Pei Qin , Yan Feng‑xia , Yang Qiao‑hong , Tian Xiao‑ying
Published: July 2023

Background
As the field of nursing science continues to evolve and higher education expands, enhancing empathy and humanistic care skills among nursing students prior to their clinical practice has become a crucial responsibility for nursing educators. To investigate the impact of the TPKCEE compound teaching model, which is based on narrative nursing, on the cultivation of empathy and humanistic care qualities among students in the ideological and political teaching of obstetrics and gynecology nursing.
Methods
A convergent mixed method design was used, which consisted of a quasi-experimental design and a semistructured interview.Data collection took place from September 2021 to February 2023. A convenience sampling method was used to select 61 fulltime third-year nursing students in a school in China (a control group of 27 and an experimental group of 34). The control group was exposed to traditional curriculum, while the experimental group used the TPCKEE teaching model based on narrative nursing. Jefferson Empathy Scale (JSE) and Caring Ability Scale (CAI) were used for quantitative assessment before and after the course, descriptive statistics and Mann-Whitney U test were used to compare the empathy and caring ability of the two groups of students, and semi-structured interviews were used for qualitative assessment. Thematic analysis was used to understand the development of students in humanistic care, empathy and learning experience.
Results
After teaching, the total score of empathy and the dimension of transposition, emotion and viewpoint, and the total score of CAI and the dimension of understanding and courage of nursing students in the control group were better than those in the control group (P < 0.05).
Conclusion
The TPCKEE teaching model based on narrative nursing effectively improves empathy, caring behavior, and classroom teaching outcomes in third-year nursing students.
Autistic Traits Predicting Videconference Fatigue: Do Social Phobia and Internet Use Disorder Mediate that Association?
Authors: Zhang Yingying
Published: July 2023

Background
The present work sets out to replicate findings linking specific psychological factors with videoconference fatigue (VC fatigue). Specifically, an earlier study demonstrated that high VC fatigue was associated with high autistic traits, high social phobia, and high tendencies toward Internet use disorder (IUD) in a German-speaking sample, and a positive association between personality trait neuroticism and VC fatigue was also observed in Western countries. Due to the replication crisis of confidence in psychology and related disciplines, replicating findings in psychological research has become increasingly important. Therefore, we aimed to replicate the relationships between these psychological factors in a Chinese sample with different cultural backgrounds relative to previous studies.
Methods
We replicated this earlier study by investigating a sample from a different cultural background, which included 408 Chinese participants (214 males, 194 females, mean age of 21.61 years). Survey data via the Internet was collected to replicate the relationships between autistic traits, social phobia, IUD, neuroticism, and VC fatigue.
Results
As expected, autistic traits, social phobia, and IUD tendencies were robustly and positively associated with VC fatigue. A similar mediation effect also indicated that the relationship between autistic traits and VC fatigue was significantly mediated by IUD tendencies. Additionally, a positive association between neuroticism and VC fatigue was also observed in the present study.
Conclusion
Psychological factors, including autistic traits, IUD and neuroticism, might be important factors that contribute to VC fatigue. The consistency of correlations across studies and cultural backgrounds underlines the validity of these relationships between psychological factors and VC fatigue.
Girdling behavior of the longhorn beetle modulates the host plant to enhance larval performance
Authors: Choi Min-Soo , Lee Juhee , Kim Jeong-Min , Kim Sang-Gyu , Joo Youngsung
Published: July 2023

Background
Preingestive behavioral modulations of herbivorous insects are abundant over insect taxa. Those behaviors are suspected to have functions such as deactivation of host plant defense, nutrient accumulation, or modulating plant-mediated herbivore interaction. However, the hypotheses have rarely been experimentally tested, especially at molecular level. To understand the functional consequence of behavioral modulation of insect herbivore, we studied the girdling behavior of Phytoecia rufiventris Gautier (Lamiinae; Cerambycidae) on its host plant Erigeron annuus L. (Asteraceae) that is performed before endophytic oviposition in the stem.
Results
The girdling behavior significantly increased the larval performance in both field monitoring and lab experiment. The upper part of the girdled stem exhibited lack of jasmonic acid induction upon larval attack, lowered protease inhibitor activity, and accumulated sugars and amino acids in compared to non-girdled stem. The modulated plant physiology allowed P. rufiventris larvae to allocate resources from detoxification to growth at the transcriptional level. The girdling behavior had no effect on the larval performance of a non-girdling longhorn beetle Agapanthia amurensis, which also feeds on the stem of E. annuus during larval phase. However, the girdling behavior decreased the preference of A. amurensis females for oviposition, which enabled P. rufiventris larvae to avoid competition with A. amurensis larvae.
Conclusions
In conclusion, the girdling behavior modulates plant physiology and morphology to provide a modulated food source for larva and hide it from the competitor. Our study implies that the insect behavior modulations can have multiple functions, providing insights into adaptation of insect behavior in context of plant-herbivore interaction.
Wave scattering in 1D: D'Alembert-type representations and a reconstruction method
Authors: Kalimeris Konstantinos , Mindrinos Leonidas
Published: July 2023

We derive the extension of the classical d'Alembert formula for the wave equation, which provides the analytical solution for the direct scattering problem for a medium with constant refractive index; this is achieved by employing results obtained via the Fokas method. This methodology is further extended to a medium with piecewise constant refractive index, providing the apparatus for the solution of the associated inverse scattering problem. Hence, we provide an exact reconstruction method which is valid for both full and phaseless data.
Post-processing technology of the five-axis additive–subtractive composite manufacturing machine tool
Authors: Qiao Peng , Tang Qingchun , Hu Tian , Zhang Han
Published: July 2023

A post-processing technology is crucial in manufacturing complex curved surface parts as a bridge between CAD/CAM and machine manufacturing. Unlike the traditional five-axis machine tool, the five-axis additive–subtractive composite manufacturing machine tool combines additive and subtractive manufacturing. This study examines the post-processing technology to coordinate and control the contour accuracy of forming parts in the composite manufacturing. First, the basic kinematic model of the five-axis additive–subtractive composite manufacturing machine tool is constructed on the basis of the inverse kinematic principle by assuming a laser nozzle coaxial powder feeding head. Second, the reasons for the incoordination of the two process coordinates are analyzed, and the criterion of process coordinate coordination is established on the basis of the five-axis general kinematic model. Finally, a special five-axis additive–subtractive composite post-processing software is developed based on a high-level language and verified by additive manufacturing, subtractive manufacturing, and process coordinate experiments. Experimental results show that the established composite post-processing model can effectively realize the contour accuracy of additive–subtractive composite manufacturing of highly complex curved parts.
Analysis and Regulatory Mechanisms of Platelet-related Genes in Patients with Ischemic Stroke
Authors: Li Yuan , Shu Yuanlu , Yu Kun , Ni Ruihan , Chu Lan
Published: July 2023

It was found that ischemic stroke (IS) was associated with abnormal platelet activity and thrombosis. However, the potential significance of platelet-related genes (PRGs) in IS still needs to be more thorough. This study extracted IS-related transcriptome datasets from the Gene Expression Omnibus (GEO) database. The target genes were obtained by intersecting the differentially expressed genes (DEGs), the module genes related to IS, and PRGs, where the key genes of IS were screened by two machine learning algorithms. The key genes-based diagnostic model was constructed. Gene set enrichment analysis (GSEA) and the immune microenvironment analyses were analyzed targeting key genes in IS. The co-expression, TF-mRNA, and competitive endogenous RNAs (ceRNA) regulatory networks were constructed to reveal the potential regulation of key genes. Potential drugs targeting key genes were predicted as well. Totals of eight target genes were obtained and were associated with immune-related functions. Four platelet-related key genes were acquired, which were related to immunity and energy metabolism. The abnormal expressions of DOCK8, GIMAP5, ICOS were determined by the quantitative real-time Polymerase Chain Reaction (qRT-PCR), and the significant correlations among these key genes were identified. Notably, hsa-miR-17-3p, hsa-miR-3158-3p, hsa-miR-423-3p, and hsa-miR-193a-8p could regulate all key genes at the same time. In addition, Caffeine, Carboplatin, and Vopratelimab were the targeted drugs of these key genes. This study identified four platelet-related key genes of IS, which might help to deepen the understanding of the role of platelet-related genes in the molecular mechanism of IS.
Spatial Self-Bending Soliton Phenomenon of (2+1) Dimensional bidirectional Sawada-Kotera Equation
Authors: Wang Jing
Published: July 2023

In this paper, we mainly study the spatial self-bending soliton phenomenon of the (2+1) dimensional bidirection-al Sawada-Kotera equation. The bilinear form of the (2+1)-dimensional bidirectional Sawada-Kotera equation is obtained by Hirota bilinear method, and then the N-soliton solution is obtained through special parameter constraints (e A js = 0), the spatial self-bending soliton is derived from the N-soliton solution, and the curvature of the spatial self-bending soliton is given furthermore, the interaction solution of spatial self-bending soliton and respiratory wave is obtained, and the interaction solution of spatial self-bending soliton and higher-order lump wave is obtained by using the method of long wave limit.