bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of hypertension and correlation with mental health in women with burning mouth syndrome: A case-control study
Authors: Canfora Federica , Calabria Elena , Pecoraro Giuseppe , Leuci Stefania , Coppola Noemi , Mazzaccara Cristina , Spirito Francesca , Aria Massimo , D'Aniello Luca , Mignogna Michele Davide , Adamo Daniela
Published: January 2023

Background

The relationship between hypertension (HTN) and chronic pain is still a matter of debate, and its prevalence in patients with burning mouth syndrome (BMS) has never been evaluated. This study aimed to assess the prevalence of HTN in women with BMS and to evaluate its relationship with potential predictors such as risk factors for cardiovascular diseases, pain, and mental health status analyzing differences with healthy women.

Methods

In total, 250 women with BMS (WBMS) were prospectively recruited and compared with an equal number of healthy women (HW) matched for age. Education, body mass index, smoke and alcohol consumption, intensity and quality of pain, and psychological profile were further investigated to identify the potential predictors of HTN. Specifically, pain assessment [the Numeric Rating Scale (NRS) and Short-Form McGill Pain Questionnaire (SF-MPQ)] and psychological assessment [Hamilton Rating Scale for Depression and Anxiety (HAM-D and HAM-A), Pittsburgh Sleep Quality Index (PSQI), and Epworth Sleepiness Scale (ESS)] was carried out for the participants.

Results

HTN was found in 128 (51.2%) WBMS and 76 (30.4%) HW (p < 0.001**). The scores of the NRS, SF-MPQ, HAM-D, HAM-A, and PSQI were statistically significantly higher in the WBMS than in the HW (p < 0.001**). A strongly linear correlation between HTN and employment status, systemic diseases, and education level (p < 0.001**) was found in WBMS, while a strong correlation between HTN and employment status, hypercholesterolemia, systemic diseases, and drug consumption was found in HW (p < 0.001**). No statistically significant correlation was found between HTN and pain, anxiety, depression, and sleep disturbances.

Conclusion

These results suggest that WBMS showed a higher prevalence of HTN compared with controls. Unemployed WBMS with lower education and other systemic comorbidities are at an increased risk of developing HTN. HTN is associated with alteration in the vascular structure and function of the brain, and these processes accelerate brain aging, which contributes to a reduction in intracortical connectivity, thus affecting the modulatory system of control of pain in patients with BMS, independently of their mental health assessment. Predictors that may underlie this association remain unclear, taking into account the differences found in HW, and should be further elucidated.
Rifting/spreading propagation interacts with preexisting transform faults: 3D geodynamic modeling
Authors: Li Hao , Liao Jie , Shen Yongqiang , Qing Jiarong , Wu Yangming , Zhao Zhongxian , Shi Xiaobin
Published: January 2023

The divergent rifting/spreading centers and the strike-slip transform faults are the essential tectonic units on Earth, the dynamic evolution of which regulates the development of rifting/spreading basins. The propagation of rifting/spreading centers may interact with pre-existing transform faults, but how they interact with each other remains enigmatic. Here we use three-dimensional geodynamical numeric models to systematically simulate the interaction between rifting/spreading propagation and the pre-existing transform faults. Our model results provide the following findings. 1) The pre-existing transform faults affect rifting/spreading propagation promoting the formation of ridge segments with an offset distance, facilitating the process of spreading of the western sea basin and restraining the propagation of the east sea basin. Yet, the evolution of the transform faults is regulated by rifting/spreading propagation, featured by the increase of its length, the change in its width along strike and the presence of lineated magmatism. 2) The initial length and orientation of the pre-existing transform faults largely affect rifting/spreading propagation, i.e., large transform fault length favors the formation of large offset between ridge segments, and oblique transform faults facilitate the formation of overlapped spreading centers. 3) Model results shed new light on the evolution of the South China Sea basin, implying that the observed ridge segments in the east and southwest sub-basins, the difference of the Zhongnan Fault Zone width along strike and the lineated volcanos along the Zhongnan Fault Zone are the results of the interaction between the rifting/spreading propagation and the pre-existing transform fault.
Study on the pore structure characteristics and damage constitutive model of sandstone under freeze-thaw conditions
Authors: Ma Junzu , Jin Jiaxu , Wu Pengfei , Qin Zhifa , Jia Baoxin
Published: January 2023

Rocks in Northwest China are often affected by the combined action of freeze-thaw and load erosion. Therefore, in order to better understand the mechanical properties of rocks in seasonal frozen areas and the meso-damage caused by freeze-thaw erosion, uniaxial compression tests, electron microscope scanning tests, X-ray diffraction tests (XRD) and mercury intrusion tests (MIP) were carried out on five sandstone samples with different freeze-thaw times, and the mechanical parameters and meso-damage characteristics of sandstone samples with different freeze-thaw times were obtained. Fractal theory was used to analyze the change in pore volume of sandstone after freeze-thaw cycles. Finally, the damage constitutive equation under the coupling action of freeze-thaw damage and load was established based on Lemaitre’s equivalent effect variation criterion. The results showed that the type of sandstone is a porous coarse-grained sandstone. With the increased freeze-thaw times, the compressive strength and cohesion of sandstone gradually decreased, and the closed pores in sandstone gradually connected, leading to the visible internal macroscopic cracks. Affected by freeze-thaw times, the volume proportion of large pores (100–1,000 µm) in sandstone gradually increased, while the volume proportion of micropores (.05–100 µm) gradually decreased. With the increased freeze-thaw times, the fractal dimension of pore volume decreased from 1.94 to 1.59. The theoretical curve can better fit the characteristic points of the stress-strain curve, which can further reveal the damage mechanism of sandstone under the coupling effects of freeze-thaw and load. The minimum error between the peak point of the experimental curve and the theoretical curve is 3.3%.
How can signaling in authentic classroom videos support reasoning on how to induce learning strategies?
Authors: Tannert Swantje , Eitel Alexander , Marder Johanna , Seidel Tina , Renkl Alexander , Glogger-Frey Inga
Published: January 2023

Classroom videos are a viable means to implement evidence-informed reasoning in teacher education in order to establish an evidence-informed teaching practice. Although learning with videos relieves pre-service teachers from acting in parallel and might reduce complexity, the material still poses higher cognitive load than written text vignettes or other traditionally used static material. In particular, the information they deliver is transient and can, therefore, easily be missed. Signaling can guide learners’ attention to central aspects of a video, thereby reducing cognitive load and enhancing learning outcomes. In the current project, pre-service teachers acquired scientific knowledge about learning strategies and their promotion in a computer-based learning environment. We explored the effect of different arrangements of signaling in classroom video-examples on conceptual knowledge and the reasoning-component of professional vision. Therefore, we conducted a set of two studies with 100 student teachers including two signal arrangements in order to investigate how signaling can help learning to reason about classroom videos. In addition, we varied if participants received information on the use of signals in advance (informed) or not (uninformed). We measured conceptual knowledge by asking participants what they knew about self-regulation strategies. Additionally, we assessed reasoning by asking participants to notice sequences in a video where teachers induced learning strategies, and to reason in what respect the observed behavior was useful to induce the strategy. Uninformed signaling did not affect the acquisition of conceptual knowledge and reasoning. Informed signaling led to significantly better conceptual knowledge than uninformed signaling. It is argued that the signal-induced extraneous load exceeded the load reduction due to the signal’s selection advantage in the uninformed conditions. In a third, exploratory study, nine participants were interviewed on the perception of different signals and indicated that spotlight and zoom-in signals foster processing of classroom videos.
Integrated lipids biomarker of the prediabetes and type 2 diabetes mellitus Chinese patients
Authors: Yang Jiaying , Wang Mei , Yang Dawei , Yan Han , Wang Zhigang , Yan Dan , Guo Na
Published: January 2023

Introduction

Dyslipidemia is a hallmark of T2DM, and as such, analyses of lipid metabolic profiles in affected patients have the potential to permit the development of an integrated lipid metabolite-based biomarker model that can facilitate early patient diagnosis and treatment.

Methods

Untargeted and targeted lipidomics approaches were used to analyze serum samples from newly diagnosed 93 Chinese participants in discovery cohort and 440 in validation cohort via UHPLC-MS and UHPLC-MS/MS first. The acid sphingomyelinase protein expression was analyzed by Western blot.

Results and Discussion

Through these analyses, we developed a novel integrated biomarker signature composed of LPC 22:6, PC(16:0/20:4), PE(22:6/16:0), Cer(d18:1/24:0)/SM(d18:1/19:0), Cer(d18:1/24:0)/SM(d18:0/16:0), TG(18:1/18:2/18:2), TG(16:0/16:0/20:3), and TG(18:0/16:0/18:2). The area under the curve (AUC) values for this integrated biomarker signature for prediabetes and T2DM patients were 0.841 (cutoff: 0.565) and 0.894 (cutoff: 0.633), respectively. Furthermore, theresults of western blot analysis of frozen adipose tissue from 3 week (prediabetes) and 12 week (T2DM) Goto–Kakizaki (GK) rats also confirmed that acid sphingomyelinase is responsible for significant disruptions in ceramide and sphingomyelin homeostasis. Network analyses of the biomarkers associated with this biosignature suggested that the most profoundly affected lipid metabolism pathways in the context of diabetes include de novo ceramide synthesis, sphingomyelin metabolism, and additional pathways associated with phosphatidylcholine synthesis. Together, these results offer new biological insights regarding the role of serum lipids in the context of insidious T2DM development, and may offer new avenues for future diagnostic and/or therapeutic research.
An online tool for predicting ovarian responses in unselected patients using dynamic inhibin B and basal antimüllerian hormone levels
Authors: Ma Congcong , Xu Huiyu , Wang Haiyan , Feng Guoshuang , Han Yong , Alpadi Kannan , Li Rong , Qiao Jie
Published: January 2023

Background

Reliable predictive models for predicting excessive and poor ovarian response in controlled ovarian stimulation (COS) is currently lacking. The dynamic (Δ) inhibin B, which refers to increment of inhibin B responding to exogenous gonadotropin, has been indicated as a potential predictor of ovarian response.

Objective

To establish mathematical models to predict ovarian response at the early phase of COS using Δinhibin B and other biomarkers.

Materials and methods

Prospective cohort study in a tertiary teaching hospital, including 669 cycles underwent standard gonadotropin releasing hormone (GnRH) antagonist ovarian stimulation between April 2020 and September 2020. Early Δinhibin B was defined as an increment in inhibin B from menstrual day 2 to day 6 through to the day of COS. Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression with 5-fold cross-validation was applied to construct ovarian response prediction models. The area under the receiver operating characteristic curve (AUC), prevalence, sensitivity, and specificity were used for evaluating model performance.

Results

Early Δinhibin B and basal antimüllerian hormone (AMH) levels were the best measures in building models for predicting ovarian hypo- or hyper-responses, with AUCs and ranges of 0.948 (0.887–0.976) and 0.904 (0.836–0.945) in the validation set, respectively. The contribution of the early Δinhibin B was 67.7% in the poor response prediction model and 56.4% in the excessive response prediction model. The basal AMH level contributed 16.0% in the poor response prediction model and 25.0% in the excessive response prediction model. An online website-based tool (http://121.43.113.123:8001/) has been developed to make these complex algorithms available in clinical practice.

Conclusion

Early Δinhibin B might be a novel biomarker for predicting ovarian response in IVF cycles. Limiting the two prediction models to the high and the very-low risk groups would achieve satisfactory performances and clinical significance. These novel models might help in counseling patients on their estimated ovarian response and reduce iatrogenic poor or excessive ovarian responses.
Sheehan syndrome: Cardiovascular and metabolic comorbidities
Authors: Laway Bashir Ahmad , Baba Mohammad Salem
Published: January 2023

Sheehan syndrome (SS) caused by postpartum hemorrhage leads to partial or complete pituitary hormone deficiency. In addition to lipid and glucose abnormalities, patients with SS have increased body fat, insulin resistance (IR), coagulation abnormalities, increased leptin concentration, low-grade inflammation, and endothelial dysfunction that predispose them to cardiovascular diseases. Untreated growth hormone (GH) deficiency, hypogonadism, and excess glucocorticoid use are considered risk factors for these abnormalities. Compared to other hypopituitary subjects, patients with SS are younger and have a longer duration of disease and severe GH deficiency. Replacement with GH in addition to standard hormone replacement improves their cardiometabolic profile.
Carbon dioxide partial pressures and emissions of the Yarlung Tsangpo River on the Tibetan Plateau
Authors: Bao Yufei , Hu Mingming , Li Shanze , Wang Yuchun , Wen Jie , Wu Xinghua , Sun Meng , Du Pengcheng
Published: January 2023

Rivers are important routes for material and energy transport between terrestrial and marine ecosystems. Recent global-scale assessments of carbon (C) have suggested that C emission fluxes to the atmosphere are comparable to the fluvial C fluxes to the ocean. However, many previous studies only collected data from inland rivers in low altitude regions. Therefore, it remains unclear how plateau rivers affect C flux. In this study, 20 monitoring sites were set up along the Yarlung Tsangpo (YT) River on the Tibetan Plateau and detailed observations were carried out in the wet and dry seasons. The riverine CO2 fluxes exhibited significant seasonal patterns which ranged from 597.12 ± 292.63 μatm in the wet season to 368.72 ± 123.50 μatm in the dry season. The CO2 emission flux (FCO2) obtained from floating chamber method, ranging from 8.44 ± 6.94 mmol m−2 d−1 in sunmmer to 3.62 ± 6.32 mmol m−2 d−1 in winter, with an average value of 6.03 mmol m−2 d−1. Generally, the river was a weak carbon source with respect to the atmosphere. However, the pCO2 and FCO2 were much lower than that for other large rivers around the globe, which were obviously restrained by the weak microbial activities due to the low primary productivity and carbonate buffer activities in the carbonate background. Carbon loss via atmosphere exchange in the YT River on the plateau accounted for 2.2% and 10.6% of the riverine dissolved carbon fluxes (67.77 × 109 mol a−1) according to the floating chamber and thin boundary layer methods, respectively. The YT River probably acts as a “pipeline” to transport weathered nutrients from the plateau to downstream areas. Our results demonstrated the characteristics of a “weak outgassing effect and a high transport flux of carbon” for the plateau river, which is different from rivers on plains. Considering the global relevance of Tibetan Plateau, further studies with enhanced spatiotemporal resolution are needed to better understand the important role of plateau rivers on carbon budgets and climate change over both regional and global cycles.
YAP-mediated mechanotransduction in urinary bladder remodeling: Based on RNA-seq and CUT&Tag
Authors: Di Xingpeng , Xiang Liyuan , Jian Zhongyu
Published: January 2023

Yes-associated protein (YAP) is an important transcriptional coactivator binding to transcriptional factors that engage in many downstream gene transcription. Partial bladder outlet obstruction (pBOO) causes a massive burden to patients and finally leads to bladder fibrosis. Several cell types engage in the pBOO pathological process, including urothelial cells, smooth muscle cells, and fibroblasts. To clarify the function of YAP in bladder fibrosis, we performed the RNA-seq and CUT&Tag of the bladder smooth muscle cell to analyze the YAP ablation of human bladder smooth muscle cells (hBdSMCs) and immunoprecipitation of YAP. 141 differentially expressed genes (DEGs) were identified through RNA-seq between YAP-knockdown and nature control. After matching with the results of CUT&Tag, 36 genes were regulated directly by YAP. Then we identified the hub genes in the DEGs, including CDCA5, CENPA, DTL, NCAPH, and NEIL3, that contribute to cell proliferation. Thus, our study provides a regulatory network of YAP in smooth muscle proliferation. The possible effects of YAP on hBdSMC might be a vital target for pBOO-associated bladder fibrosis.
Construction and verification of a novel prognostic risk model for kidney renal clear cell carcinoma based on immunity-related genes
Authors: Liu Yufeng , Wu Dali , Chen Haiping , Yan Lingfei , Xiang Qi , Li Qing , Wang Tao
Published: January 2023

Background: Currently, there are no useful biomarkers or prognostic risk markers for the diagnosis of kidney renal clear cell carcinoma (KIRC), although recent research has shown that both, the onset and progression of KIRC, are substantially influenced by immune-associated genes (IAGs).

Objective: This work aims to create and verify the prognostic value of an immune risk score signature (IRSS) based on IAGs for KIRC using bioinformatics and public databases.

Methods: Differentially expressed genes (DEGs) related to the immune systems (IAGs) in KIRC tissues were identified from The Cancer Genome Atlas (TCGA) databases. The DEGs between the tumor and normal tissues were identified using gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses. Furthermore, a prognostic IRSS model was constructed and its prognostic and predictive performance was analyzed using survival analyses and nomograms. Kidney renal papillary cell carcinoma (KIRP) sets were utilized to further validate this model.

Results: Six independent immunity-related genes (PAEP, PI3, SAA2, SAA1, IL20RB, and IFI30) correlated with prognosis were identified and used to construct an IRSS model. According to the Kaplan-Meier curve, patients in the high-risk group had significantly poorer prognoses than those of patients in the low-risk group in both, the verification set (p <0.049; HR = 1.84; 95% CI = 1.02–3.32) and the training set (p < 0.001; HR = 3.12, 95% CI = 2.23–4.37). The numbers of regulatory T cells (Tregs) were significantly positively correlated with the six immunity-related genes identified, with correlation coefficients were 0.385, 0.415, 0.399, 0.451, 0.485, and 0.333, respectively (p <0.001).

Conclusion: This work investigated the association between immune infiltration, immunity-related gene expression, and severity of KIRC to construct and verify a prognostic risk model for KIRC and KIRP.
Dupilumab for the treatment of prurigo nodularis: A systematic review
Authors: Cao Peng , Xu Wenjing , Jiang Shuyi , Zhang Litao
Published: January 2023

Background

Conventional treatment techniques have limited efficacy and more side effects in the treatment of prurigo nodularis. The better alternative treatment option for better outcomes of the disease is dupilumab.

Objective

The objective of this study was to systematically review dupilumab-related treatment outcomes in prurigo nodularis.

Methods

Several databases like Embase, PubMed, Web of Science, and Cochrane library were searched for data acquisition on October 8, 2022. Based on Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines, 24 publications were included in this study.

Results

After 4,12,16 and more than 16 weeks of dupilumab treatment, 8.3% (n=5/60), 34.4% (n=11/32), 3.6% (n=2/56), and 45.3% (n=29/64) of patients had complete remission, respectively. In addition, 85.0% (n=51/60), 59.4% (n=19/32), 83.9% (n=47/56), and 43.8% (n=28/64) had partial remission, respectively. Moreover, 6.7% (n=4/60), 6.3% (n=2/32), 12.5% (n=7/56), and 10.9% (n=7/64) showed no remission, respectively, and significant reduction of numeric rating scale itch intensity (from 9.0 to 4.9, 2.1, 2.8, 0.9) was attained. There were no serious adverse events observed during treatment, but the most common event observed was conjunctivitis (12.6%, n=15/119).

Conclusions

Dupilumab has definite effectiveness and safety in prurigo nodularis treatment.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO, identifier (CRD42022365802).
Ten-year follow-up of infliximab treatment for uveitis in Behçet disease patients: A multicenter retrospective study
Authors: Takeuchi Masaru , Usui Yoshihiko , Namba Kenichi , Keino Hiroshi , Takeuchi Masaki , Takase Hiroshi , Kamoi Koju , Hase Keitaro , Ito Takako , Nakai Kei , Maruyama Kazuichi , Kobayashi Eri , Mashimo Hisashi , Sato Tomohito , Ohguro Nobuyuki , Hori Junko , Okada Annabelle A. , Sonoda Koh-hei , Mizuki Nobuhisa , Goto Hiroshi
Published: January 2023

Purpose

To evaluate 10-year outcome of infliximab (IFX) treatment for uveitis in Behçet disease (BD) patients using a standardized follow-up protocol.

Design

Retrospective longitudinal cohort study.

Participants

140 BD uveitis patients treated with IFX enrolled in our previous study.

Methods

Medical records were reviewed for demographic information, duration of IFX treatment, number of ocular attacks before IFX initiation, best corrected visual acuity (VA) at baseline and 1, 2, 3, 4, 5, and 10 years after IFX initiation, uveitis recurrence after IFX initiation and main anatomical site, concomitant therapies, and adverse events (AEs).

Main outcome measures

10-year IFX continuation rate and change in LogMAR VA.

Results

Of 140 BD patients, 106 (75.7%) continued IFX treatment for 10 years. LogMAR VA improved gradually after initiation of IFX, and the improvement reached statistical significance from 2 years of treatment. Thereafter, significant improvement compared with baseline was maintained until 10 years, despite a slight deterioration of logMAR VA from 5 years. However, eyes with worse baseline decimal VA < 0.1 showed no significant improvement from baseline to 10 years. Uveitis recurred after IFX initiation in 50 patients (recurrence group) and did not recur in 56 (non-recurrence group). Ocular attacks/year before IFX initiation was significantly higher in the recurrence group (2.82 ± 3.81) than in the non-recurrence group (1.84 ± 1.78). In the recurrence group, uveitis recurred within 1 year in 58% and within 2 years in 74%. Seventeen patients (34%) had recurrent anterior uveitis, 17 (34%) had posterior uveitis, and 16 (32%) had panuveitis, with no significant difference in VA outcome. In addition, logMAR VA at 10 years did not differ between the recurrence and non-recurrence groups. AEs occurred among 43 patients (30.7%), and 24 (17.1%) resulted in IFX discontinuation before 10 years.

Conclusions

Among BD patients with uveitis who initiated IFX, approximately 75% continued treatment for 10 years, and their VA improved significantly and was maintained for 10 years. Uveitis recurred in one-half of the patients, but visual acuity did not differ significantly from the patients without recurrence.
Genetic removal of synaptic Zn2+ impairs cognition, alters neurotrophic signaling and induces neuronal hyperactivity
Authors: Vogler Emily C. , Mahavongtrakul Matthew , Sarkan Kristianna , Bohannan Ryan C. , Catuara-Solarz Silvina , Busciglio Jorge
Published: January 2023

Vesicular Zn2+ (zinc) is released at synapses and has been demonstrated to modulate neuronal responses. However, mechanisms through which dysregulation of zinc homeostasis may potentiate neuronal dysfunction and neurodegeneration are not well-understood. We previously reported that accumulation of soluble amyloid beta oligomers (AβO) at synapses correlates with synaptic loss and that AβO localization at synapses is regulated by synaptic activity and enhanced by the release of vesicular Zn2+ in the hippocampus, a brain region that deteriorates early in Alzheimer's disease (AD). Significantly, drugs regulating zinc homeostasis inhibit AβO accumulation and improve cognition in mouse models of AD. We used both sexes of a transgenic mouse model lacking synaptic Zn2+ (ZnT3KO) that develops AD-like cognitive impairment and neurodegeneration to study the effects of disruption of Zn2+ modulation of neurotransmission in cognition, protein expression and activation, and neuronal excitability. Here we report that the genetic removal of synaptic Zn2+ results in progressive impairment of hippocampal-dependent memory, reduces activity-dependent increase in Erk phosphorylation and BDNF mRNA, alters regulation of Erk activation by NMDAR subunits, increases neuronal spiking, and induces biochemical and morphological alterations consistent with increasing epileptiform activity and neurodegeneration as ZnT3KO mice age. Our study shows that disruption of synaptic Zn2+ triggers neurodegenerative processes and is a potential pathway through which AβO trigger altered expression of neurotrophic proteins, along with reduced hippocampal synaptic density and degenerating neurons, neuronal spiking activity, and cognitive impairment and supports efforts to develop therapeutics to preserve synaptic zinc homeostasis in the brain as potential treatments for AD.
Potential mechanisms and modulators of food intake during pregnancy
Authors: Waclawek Theresa , Park Soyoung Q.
Published: January 2023

Dietary choice during pregnancy is crucial not only for fetal development, but also for long-term health outcomes of both mother and child. During pregnancy, dramatic changes in endocrine, cognitive, and reward systems have been shown to take place. Interestingly, in different contexts, many of these mechanisms play a key role in guiding food intake. Here, we review how food intake may be impacted as a function of pregnancy-induced changes across species. We first summarize changes in endocrine and metabolic signaling in the course of pregnancy. Then, we show how these may be related to cognitive function and reward processing in humans. Finally, we link these to potential drivers of change in eating behavior throughout the course of pregnancy.
Encephalitis and cytokine storm secondary to respiratory viruses in children: Two case reports
Authors: Santos Pollyana C. P. , Holloway Adrian J. , Custer Jason W. , Alves Tomaz , Simon Liliana
Published: January 2023

Introduction

Encephalitis is a syndrome characterized by brain damage secondary to an inflammatory process that is manifested by cognitive impairment and altered cerebral spinal fluid analysis; it may evolve with seizures and coma. Despite viral infections representing the main cause of encephalitis in children, respiratory syncytial virus (RSV) and parainfluenza virus are mostly associated with respiratory presentations. Uncommonly, the inflammatory phenomena from encephalitis secondary to viral agents may present with an exacerbated host response, the so-called cytokine storm. The link between these infectious agents and neurologic syndromes resulting in a cytokine storm is rare, and the underlying pathophysiology is still poorly understood.

Case presentation

A 5-year-old girl and a 2-year-old boy infected with parainfluenza and RSV, respectively, were identified through nasopharyngeal polymerase chain reaction. They were admitted into the pediatric intensive care unit due to encephalitis and multiple organ dysfunction manifested with seizures and hemodynamic instability. Magnetic resonance imaging findings from the first patient revealed a bilateral hypersignal on fluid-attenuated inversion recovery in the cerebral hemispheres, especially in the posterior parietal and occipital regions. The girl also had elevated IL-6 levels during the acute phase and evolved with a fast recovery of the clinical presentations. The second patient progressed with general systemic complications followed by cerebral edema and death.

Conclusion

Encephalitis secondary to respiratory viral infection might evolve with cytokine storm and multiorgan inflammatory response in children.
Second generation androgen receptor antagonist, TQB3720 abrogates prostate cancer growth via AR/GPX4 axis activated ferroptosis
Authors: Zhang Zhongqing , Xie Tianlei , Zhang Shun , Yin Haoli , Zhang Xuyu , Zhang Siyuan , Chen Wei , Yu Ding , Qiu Xuefeng , Zhao Wei , Guo Hongqian , Zhuang Junlong
Published: January 2023

Purpose: Prostate cancer (PCa) poses a great threat to humans. The study aimed to evaluate the potential of TQB3720 in promoting ferroptosis to suppress prostate cancer, providing a theoretical basis for PCa therapy.

Methods: PCa cells and nude mice models were divided into TQB3720, enzalutamide (ENZ), and control groups. Sulforhodamine B assay, colony formation assessment, organoids culture system, and the CCK8 assay were used for detecting proliferation. Western blot assay was processed to detect the expression of androgen receptor (AR), ferroptosis, and apoptosis-related genes. Flow cytometry was applied to measure the intracellular ROS levels. ELISA was performed to determine the cellular oxidized glutathione (GSSG) and malondialdehyde (MDA) levels. RT-qPCR was conducted to detect the mRNA expression of genes in AR signaling. BODIPYTM™ 581/591 was processed for detection of intracellular lipid peroxidation levels. The interaction of AR with other translational factor complex proteins was explored using Co-immunoprecipitation (Co-IP), and the chromatin immunoprecipitation (ChIP) assay was performed to detect the binding of AR-involved translational complex to downstream genes promoter. Luciferase reporter assay was conducted to examine the translation activity of GPX4 promoter, and immunohistochemistry (IHC) was conducted to analyze the levels of c-MYC, Ki-67 and AR in TQB3720-treated cancer tissues.

Results: Here, we found TQB3720 inhibits the growth of prostate cancer in vitro and in vivo. TQB3720 treatment induced intracellular levels of GSSG and MDA significantly, by which hints AR antagonist caused ferroptosis-related cell death. Moreover, molecular evidence shown TQB3720 regulates downstream of AR signaling by binding AR resulting in inhibition of AR entry into the nucleus. Additional, we also proved that TQB3720 abrogates the interaction between AR and SP1 and leads to decrease GPX4 transcription.

Conclusion: TQB3720 promotes ferroptosis in prostate cancer cells by reducing the AR/SP1 transcriptional complex binding to GPX4 promoter. As a result, it is suggested to be a potential drug for clinic prostate cancer treatment.
Chiral uncertainties in ab initio elastic nucleon-nucleus scattering
Authors: Baker Robert B. , Burrows Matthew , Elster Charlotte , Launey Kristina D. , Maris Pieter , Popa Gabriela , Weppner Stephen P.
Published: January 2023

The effective interaction between a nucleon and a nucleus is one of the most important ingredients for reaction theories. Theoretical formulations were introduced early by Feshbach and Watson, and efforts of deriving and computing those ‘optical potentials’ in a microscopic fashion have a long tradition. However, only recently the leading order term in the Watson multiple scattering approach could be calculated fully ab initio, meaning that the same nucleon-nucleon (NN) interaction enters both the structure as well as the reaction pieces on equal footing. This allows the uncertainties from the underlying chiral effective NN interaction to be systematically explored in nucleon-nucleus elastic scattering observables. In this contribution the main ingredients for arriving at the ab initio leading order of the effective nucleon-nucleus interaction in the Watson approach will be reviewed. Concentrating on one specific chiral NN interaction from the LENPIC collaboration and light nuclei with a 0+ ground state, the leading order nucleon-nucleus interaction is calculated using up to the third chiral order (N2LO) in the nucleon-nucleon potential, and elastic scattering observables are extracted. Then pointwise as well as correlated uncertainty quantification is used for the estimation of the chiral truncation error. Elastic scattering observables for 4He, 12C, and 16O for between 65 and 200 MeV projectile energy will be analyzed.
Assessing proprioception through time-variability properties of acceleration
Authors: Montull Lluc , Borrallo Alex , Almarcha Maricarmen , Balagué Natàlia
Published: January 2023

Proprioception is a crucial property for movement stability and balance, but its current assessment, based on clinical testing, lacks precision and adequacy in real contexts. This study proposes assessing proprioception and its sensitivity to training effects through acceleration time series recorded during two slackline experiments. In the first experiment, slackliners of different expertise (highly and poorly trained) had to walk on a slackline for 30 s. In the second, twelve beginners had to balance up on the slackline for at least 11 s before and after a training process. Acceleration time series were recorded in body components (legs and centre of mass) and the slackline. The acceleration fluctuations were analysed through Detrended Fluctuation Analysis. The obtained Hurst (H)-exponents were compared between both groups (first experiment) and before and after training (second experiment) using Whitney and Wilcoxon tests, respectively. The values of H-exponents were lower in the highly trained group (Z = −2.15, p = 0.03) (first experiment), and in the post-training conditions (Z = −2.35, p = 0.02) (second experiment). These results suggest better motor and proprioceptive control with training status. Hence, the time-variability structure of acceleration in real contexts, like slackline tasks, is proposed as an objective measure of proprioception and its training effects.
Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes
Authors: Zhao Xijuan , Zhang Huiling , Liu Tengfei , Zhao Yanan , Hu Xinxi , Liu Shengxuan , Lin Yuan , Song Botao , He Changzheng
Published: January 2023

Anthocyanin biosynthesis is affected by light, temperature, and other environmental factors. The regulation mode of light on anthocyanin synthesis in apple, pear, tomato and other species has been reported, while not clear in potato. In this study, potato RM-210 tubers whose peel will turn purple gradually after exposure to light were selected. Transcriptome analysis was performed on RM-210 tubers during anthocyanin accumulation. The expression of StMYBA1 gene continued to increase during the anthocyanin accumulation in RM-210 tubers. Moreover, co-expression cluster analysis of differentially expressed genes showed that the expression patterns of StMYBA1 gene were highly correlated with structural genes CHS and CHI. The promoter activity of StMYBA1 was significantly higher in light conditions, and StMYBA1 could activate the promoter activity of structural genes StCHS, StCHI, and StF3H. Further gene function analysis found that overexpression of StMYBA1 gene could promote anthocyanin accumulation and structural gene expression in potato leaves. These results demonstrated that StMYBA1 gene promoted potato anthocyanin biosynthesis by activating the expression of structural genes under light conditions. These findings provide a theoretical basis and genetic resources for the regulatory mechanism of potato anthocyanin synthesis.
The predictors of spiritual dryness among Iranian cancer patients during the COVID-19 pandemic
Authors: Farahani Azam Shirinabadi , Kerdar Sara Hamideh , Ashrafizadeh Hadis , Büssing Arndt , Mehrnoush Nasrin , Akbari Mohammad Esmaeil , Karami Maryam , Tajalli Salehe , Mojen Leila Khanali , Rassouli Maryam
Published: January 2023

Background

Spiritual struggles affect the wellbeing of religious people. Among them are strugglers with God which is perceived as non-responsive and distant. These perceptions were so far analyzed predominantly in Western societies with a Christian background, but not in Muslims from Iran. The aim of this study was to determine the predictors of spiritual dryness among cancer patients in Iran during the COVID-19 pandemic.

Methods

Cross-sectional study with standardized questionnaires (i.e., Spiritual Dryness Scale, WHO-5, BMLSS-10, Awe/Gratitude Scale) among 490 cancer patients (mean age 49.50 ± 14.92 years) referring to the selected educational hospitals in Tehran (the capital of Iran), who were selected through convenience sampling and based on the inclusion criteria, enrolled between December 2020–May 2021. Data analysis was done using SPSS software version 26 and the statistical methods including calculating the mean and the standard deviation, correlation coefficients, as well as regression analysis.

Results

The overall experience of spiritual dryness was perceived regularly in 10.2% of Iranian cancer patients, sometimes in 22.9%, rarely in 22.9%, and never in 43.3%. The mean ± SD was 25.66 ± 5.04, and the scores ranged from 10 to 55. A higher score means greater spiritual dryness. The strongest predictors of spiritual dryness were praying activities Furthermore, the perception of burden due to the pandemic was positively correlated with spiritual dryness. Moreover, each 1 unit increase in its score changed the spiritual dryness score by 0.2 units. The regression of spirituality-related indicators, demographic-clinical variables, and health-related behaviors accounted for 21, 6, and 4% of the total SDS variance, respectively. These findings show that with an increase in praying, performing daily prayers, and the indicators related to spirituality, spiritual dryness will decrease. Most patients were able to cope with these phases often or even regularly, while 31.1% were never or rarely only able to cope.

Conclusion

The results of this study showed that in times of crisis, cancer patients’ faith and confidence in God could be challenged. It is not the disease itself which seems to be associated with this form of crisis, but their religious practices. Therefore, it is necessary to support these patients during their struggle, especially as spirituality is one of the best approaches to cope with the disease.
Intergenerational residential school attendance and increased substance use among First Nation adults living off-reserve: An analysis of the aboriginal peoples survey 2017
Authors: Toombs Elaine , Lund Jessie I. , Mushquash Aislin R. , Mushquash Christopher J.
Published: January 2023

Introduction

The Truth and Reconciliation Commission of Canada (TRCC) published 94 Calls to Action in 2015 to address long-term, intergenerational effects of the residential school system, highlighting the pervasive impact of colonialism on the wellbeing of Indigenous peoples in Canada. Indeed, research with Indigenous populations in Canada has captured that prior experiences of residential schools contributes to the intergenerational transmission of mental and physical health disparities. Despite these studies, further research is needed that contextualizes the influence of residential schools within broader frameworks that consider Indigenous social determinants of health in Canada. As such, the purpose of the present study was to examine patterns of substance use and mental and physical health among individuals with a history of residential school attendance (RSA) and individuals reporting parent or two-generation (parent and grandparent) RSA.

Method

Data from the Aboriginal Peoples Survey (2017), involving 10,030 First Nations individuals living off reserve, were analyzed.

Results

Self-reported mental and physical health scores were significantly lower among those had attended residential schools, whose parents attended residential schools, and whose grandparents attended residential schools, when compared to those who did not. Further, family RSA was associated with increased substance use among participants, though the findings were variable based on sex and specific substance analyzed. Meanwhile, individual and family RSA was not associated with increased likelihood of a mental health diagnosis.

Discussion

These findings provide additional support for how both parental and two-generation family histories of RSA are associated with individual physical and mental health outcomes. Further, these findings articulate the need for the TRCC's Calls to Action to be actually implemented, including community-based approaches that harness the strength of Indigenous people and communities who aim to close the gap in these health disparities for their children and families.
Survey on public awareness, attitudes and self-protective behavior adoption in different periods of COVID-19
Authors: Fang Fang , Chen Sijie , Geng Xianhui , Kiprop Emmanuel
Published: January 2023

Introduction

The outbreak and spread of the pandemics have been an issue of critical concern globally, posing a significant threat to the health sector globally. This study aimed to examine the basic knowledge and attitudes toward the recommended protective measures at different times, respond to the COVID-19 pandemic, and provide recommendations for developing targeted strategies and measures for preventing and controlling public health emergencies.

Methods

The study used self-filled questionnaires to examine the public's knowledge, attitudes, and practices on COVID-19 at two different period, from 20 to 31 March 2020 (the beginning period) and 22–27 April 2022 (the regular epidemic prevention and control period). Descriptive and quantitative analyses were used for statistical analysis.

Results and discussion

The survey collected 2375 valid questionnaires. A comparison of the two periods reveals that as the epidemic continued over a long period, the level of knowledge, attitudes toward preventive measures, risk perceptions, and adoption behavior of the respondents at the beginning of the epidemic were significantly higher than during the regular epidemic prevention and control period. With the upsurge in the spread of the epidemic, the public needs a multi-channel, targeted, and all-round guidance and information on prevention and control of the COVID-19, and internalizes knowledge into individual's behavior of actively responding to diseases.When the epidemic lasts for a long time, the relevant agencies should strengthen their monitoring role to promote public compliance with the recommended measures.
Effects of functional limitations and activities of daily living on the mortality of the older people: A cohort study in China
Authors: Gao Yumeng , Du Liang , Cai Jianping , Hu Tingfa
Published: January 2023

Introduction

Prevalence of functional limitations (FLs) and disabled activities of daily living (ADL) cause heavy burdens to the health of the older people. Stratified by gender, this study aimed to explore the effects of FL and ADL on the mortality of the older people in China, and the mechanism was then discussed.

Methods

We used survey data of a prospective 10-year cohort (2008–2018), from the China Longitudinal Healthy Longevity Survey (CLHLS). The primary outcome was all-cause mortality, and Independent variables included FL, basic ADL (BADL), and instrumental ADL (IADL). Covariates involved socio-demographic characteristics, health-related behaviors, and health status of the participants in the CLHLS project.

Results

There were 967 (19.20%) male and 2,235 (32.36%) female older people performed functional limitations, and their survival time was 60.98 (SE = 0.66) and 55.19 (SE = 0.55) months, respectively. Old adults with FL had significantly poorer survival than the ones without (Log-rank test, P < 0.001). Weibull regression suggested that FL (P < 0.001), abnormal BADL (P < 0.001) and IADL (P < 0.001) were negatively associated with the survival of the older people. Further analysis showed that BADL and IADL performed significantly mediating roles in the relation of FL and survival time in old adults; additionally, for female older people, BADL also exhibited a significant moderating role in the effect of FL on survival.

Conclusions

Prevalence of FL was serious among the older people in China, especially for the women. Disabilities of BADL and IADL and FL were negatively associated with the survival time of the older people in China. Regarding the effect of FL on survival, BADL and IADL performed significantly mediating roles, and the moderating role of BADL existed only for the female. These suggested evidence to implement strategies to maintain health in the older people.
Short-term effect of ambient air pollution on outpatient visits for children in Guangzhou, China
Authors: Chen Sili , Xu Binhe , Shi Tongxing , Yang Qiaoyuan
Published: January 2023

This study examined the short-term relationship between ambient air pollutants and children's outpatient visits, and identified the effect of modifications by season. Daily recordings of air pollutants (CO, NO2, O3, SO2, PM10, and PM2.5) and children's outpatient visit data were collected in Guangzhou from 2015 to 2019. A generalized additive model adjusted for potential confounding was introduced to verify the association between ambient air pollution and outpatient visits for children. Subgroup analysis by season was performed to evaluate the potential effects. A total of 5,483,014 children's outpatient visits were recorded. The results showed that a 10 μg/m3 increase in CO, NO2, O3, SO2, PM10, and PM2.5 corresponded with a 0.19% (95% CI: 0.15–0.24%), 2.46% (2.00–2.92%), 0.27% (0.07–0.46%), 7.16% (4.80–9.57%), 1.16% (0.83–1.49%), and 1.35% (0.88–1.82%) increase in children's outpatient visits on the lag0 of exposure, respectively. The relationships were stronger for O3, PM10, and PM2.5 in the warm seasons, and for CO, NO2, and SO2 in the cool seasons. When adjusting for the co-pollutants, the effects of CO, NO2, and PM10 were robust. The results of this study indicate that six air pollutants might increase the risk of children's outpatient visits in Guangzhou, China, especially in the cool season.