bluuebunny/crossref_metadata_2025 huggingface dataset:
A Green Treatment Mitigates the Limitations of Coffee Silver Skin as a Filler for PLA/PBSA Compatibilized Biocomposites
Authors: Perin Davide , Dorigato Andrea , Bertoldi Erica , Fambri Luca , Fredi Giulia
Published: December 2023

The development of fully renewable and biodegradable composites for short-term applications was pursued by combining a compatibilized poly(lactic acid) (PLA)/poly(butylene succinate-co-adipate) (PBSA) (60:40 wt:wt) blend with coffee silver skin (CSS), an industrial byproduct from coffee processing. An epoxy-based reactive agent (Joncryl ADR-4468) was added as a compatibilizer. CSS was incorporated at 5, 10, and 20 wt% in the blend both in the as-received state and after a simple thermal treatment in boiling water, which was performed to mitigate the negative impact of this filler on the rheological and mechanical properties of the blend. The CSS treatment effectively increased the filler degradation temperature of 30–40 °C, enabling stable melt processing of the composites. It also improved filler–matrix adhesion, resulting in enhanced impact properties (up to +172% increase in impact energy compared to the untreated filler). Therefore, treated CSS demonstrated potential as an effective green reinforcement for PLA/PBSA blends for rigid packaging applications. Future works will focus on studying suitable surface modification of CSS to further increase the interfacial interaction and the tensile quasi-static properties, to fully exploit the capabilities of this renewable material toward the development of eco-friendly composites.
Enhancing Flame Retardancy and Smoke Suppression in Epoxy Resin Composites with Sulfur–Phosphorous Reactive Flame Retardant
Authors: Ma Xulong , Kang Ni , Zhang Yonghang , Min Yang , Yang Jianhua , Ban Daming , Zhao Wei
Published: December 2023

The presence of massive amounts of toxic volatiles and smoke during combustion is a very serious problem facing epoxy resin (EP) composites. Therefore, flame retardants (FRs) can simultaneously enhance flame retardancy and reduce the release of smoke and fatal gases. Herein, a novel sulfur–phosphorous reactive flame retardant (SPMS) was synthesized for epoxy resin. The high efficiency of smoke suppression and flame retardancy of the EP/SPMS-APP hybrid was investigated using a cone calorimeter, a vertical burning test, and limited oxygen index measurements. Compared with those of pure EP, the composite with 20 wt% SPMS-APP reduced the peak heat release rate (pHRR), the peak smoke production rate (SPR), and total smoke production rate (TSR) by 82%, 94%, and 84%, respectively. The results showed a remarkable suppressed effect of alleviating the fire hazard of EP using a sulfur–phosphorus flame retardant.
Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Fingerprinting and Chemometrics for Coffee Classification and Authentication
Authors: Núñez Nerea , Saurina Javier , Núñez Oscar
Published: December 2023

Nowadays, the quality of natural products is an issue of great interest in our society due to the increase in adulteration cases in recent decades. Coffee, one of the most popular beverages worldwide, is a food product that is easily adulterated. To prevent fraudulent practices, it is necessary to develop feasible methodologies to authenticate and guarantee not only the coffee’s origin but also its variety, as well as its roasting degree. In the present study, a C18 reversed-phase liquid chromatography (LC) technique coupled to high-resolution mass spectrometry (HRMS) was applied to address the characterization and classification of Arabica and Robusta coffee samples from different production regions using chemometrics. The proposed non-targeted LC-HRMS method using electrospray ionization in negative mode was applied to the analysis of 306 coffee samples belonging to different groups depending on the variety (Arabica and Robusta), the growing region (e.g., Ethiopia, Colombia, Nicaragua, Indonesia, India, Uganda, Brazil, Cambodia and Vietnam), and the roasting degree. Analytes were recovered with hot water as the extracting solvent (coffee brewing). The data obtained were considered the source of potential descriptors to be exploited for the characterization and classification of the samples using principal component analysis (PCA) and partial least squares–discriminant analysis (PLS-DA). In addition, different adulteration cases, involving nearby production regions and different varieties, were evaluated by pairs (e.g., Vietnam Arabica—Vietnam Robusta, Vietnam Arabica—Cambodia and Vietnam Robusta—Cambodia). The coffee adulteration studies carried out with partial least squares (PLS) regression demonstrated the good capability of the proposed methodology to quantify adulterant levels down to 15%, accomplishing calibration and prediction errors below 2.7% and 11.6%, respectively.
Pleiotropic Potential of Evernia prunastri Extracts and Their Main Compounds Evernic Acid and Atranorin: In Vitro and In Silico Studies
Authors: Studzińska-Sroka Elżbieta , Bulicz Magdalena , Henkel Marika , Rosiak Natalia , Paczkowska-Walendowska Magdalena , Szwajgier Dominik , Baranowska-Wójcik Ewa , Korybalska Katarzyna , Cielecka-Piontek Judyta
Published: December 2023

Evernia prunastri is a lichen widely distributed in the Northern Hemisphere. Its biological properties still need to be discovered. Therefore, our paper focuses on studies of E. prunastri extracts, including its main metabolites evernic acid (EA) or atranorin (ATR). Phytochemical profiles using chromatographic analysis were confirmed. The antioxidant activity was evaluated using in vitro chemical tests and in vitro enzymatic cells-free tests, namely superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT). The anti-inflammatory potential using cyclooxygenase-2 (COX-2) and hyaluronidase were determined. The neuroprotective potential using acetylcholinesterase, (AChE), butyrylcholinesterase (BChE), and tyrosinase (Tyr) was estimated. The hypoglycemic activity was also confirmed (α-glucosidase). Principal component analysis was performed to determine the relationship between the biological activity of extracts. The inhibitory effect of EA and ATR on COX-2 AChE, BChE, Tyr, and α-glucosidase was evaluated using molecular docking techniques and confirmed for EA and ATR (besides α-glucosidase). The penetration of EA and ATR from extracts through the blood–brain barrier was confirmed using the parallel artificial membrane permeability assay blood–brain barrier test. In conclusion, depending on chemical surroundings and the concentration, the E. prunastri extracts, EA or ATR, showed attractive pleiotropic properties, which should be further investigated.
Chiroptically Active Multi-Modal Calcium Carbonate-Based Nanocomposites
Authors: Donnelly Fearghal C. , Purcell-Milton Finn , Caffrey Eoin , Branzi Lorenzo , Stafford Shelley , Alhammad Faisal Ali , Cleary Olan , Ghariani Munirah , Kuznetsova Vera , Gun’ko Yurii K.
Published: December 2023

The development of multimodal nano- and micro-structures has become an increasingly popular area of research in recent years. In particular, the combination of two or more desirable properties within a single structure opens multiple opportunities from biomedicine, sensing, and catalysis, to a variety of optical applications. Here, for the first time, we report the synthesis and characterization of multimodal chiroptically active CaCO3 nanocomposites. These composites have been prepared by a modified microemulsion method in the presence of an amino acid (cysteine). Following this, additional modalities have been introduced by loading the composites with luminescent nanoparticles or doping with Eu3+ ions. The luminescent composites have been produced by the incorporation of CuInZnS/ZnS or CdSe@ZnS/ZnS core/shell quantum dots, or via doping with trivalent europium. In this manner, we have produced chiroptically active composites with orange, green, and red luminescence. Overall, this work demonstrates the unique advantage and potential of our approach and new class of chiroptically active CaCO3 nanocomposites, which display tunable functionality to specific requirements via the incorporation of desired ions, nanoparticles, and chirality of the structure.
Nanoplasmonics in Catalysis for Energy Technologies: The Concept of Plasmon-Assisted Molecular Catalysis (PAMC)
Authors: Moularas Constantinos , Gemenetzi Aikaterini , Deligiannakis Yiannis , Louloudi Maria
Published: December 2023

The utilization of plasmonic nanomaterials in catalytic technologies is an emerging research field with foreseeable applications in energy-catalytic technologies. On this front, the coupling of plasmonic nanomaterials with molecular catalysts is a newly approached, thus far unexploited field, that we discuss herein. In the present mini review, we contrast the case where the plasmonic particle itself is the catalytic center against the case where the plasmonic particle acts as a co-catalyst for an operational catalytic system. In the first part, we present an outline of the key phenomena in nanoplasmonics, and their potential implications in catalytic processes. The concepts of hot electrons, hot holes, and the dynamics of their generation and transfer are reviewed, as are the contribution of near-field and photothermal effects to catalytic processes. All these plasmonic-phenomena are then discussed in conjunction with representative catalytic systems from the literature.
Prolonged Fasting Induces Histological and Ultrastructural Changes in the Intestinal Mucosa That May Reduce Absorption and Revert after Enteral Refeeding
Authors: Nunes Gonçalo , Guimarães Marta , Coelho Hélder , Carregosa Ricardo , Oliveira Cátia , Pereira Sofia S. , Alves de Matos António , Fonseca Jorge
Published: December 2023

Background: Malnutrition is usual in patients referred for endoscopic gastrostomy (PEG). Refeeding syndrome is rarely observed in PEG-fed patients, which could possibly be associated with reduced absorption induced by prolonged starvation. Objective: In patients submitted to PEG after a significant period of fasting, the present study aims to: 1. evaluate the histological/ultrastructural initial changes in the intestinal mucosa, potentially associated with reduced absorption, and 2. assess if these changes could reverse with enteral refeeding. Methods: The present study is an observational, prospective, controlled study. Adult patients with ingestion below 50% of daily needs for at least one month and/or diagnosis of malnutrition were enrolled. Duodenal biopsies were taken at baseline and after 3–6 months of PEG feeding, which then underwent histological/ultrastructural analysis. Random healthy individuals were used as controls. Results: A total of 30 patients (16 men/14 women) aged 67.1 ± 13.5 years were included. Malnutrition was found in 40% of patients. Approximately 14 patients completed follow-up during both periods (46.7%). At baseline: duodenal mucosal atrophy was evident in three patients (10%); the median villi length (MVL) was 0.4 mm (0.25–0.6 mm), with it being shorter than the controls, which was 0.6 mm (0.4–0.7 mm) (p = 0.006); ultrastructural changes included focal shortening, bending, and disruption of enterocyte microvilli, the presence of citoplasmatic autophagic vacuoles, dilation and vesiculation of the smooth endoplasmic reticulum, and the presence of dilated intercellular spaces with basement membrane detachment. After refeeding, most patients displayed normal histology (92.9%) and increase MVL (p < 0.001), ultrastructural changes disappeared, and enterocytes resumed a normal appearance, although retaining scarce, small, dense bodies in apical regions from the evolution of previous autophagy. Conclusions: Prolonged fasting induces histological and ultrastructural changes in the intestinal mucosa that may reflect impaired absorption in the early post-PEG period. These changes were reverted after refeeding with enteral nutrition.
Different Associations of Coffee Consumption with the Risk of Incident Metabolic Dysfunction-Associated Steatotic Liver Disease and Advanced Liver Fibrosis
Authors: Lee Jun-Hyuk , Park JooYong , Ahn Sang Bong
Published: December 2023

Although coffee has a potential hepatoprotective effect, evidence of the relationship between coffee consumption and metabolic dysfunction-associated steatotic liver disease (MASLD) remains conflicting. There is limited evidence regarding the most appropriate coffee intake to prevent advanced liver fibrosis (ALF) in patients with MASLD. We investigated the effect of coffee consumption on MASLD and ALF among 5266 participants without MASLD and 1326 with MASLD but without ALF. Participants were grouped by coffee intake: non-consumers, >0 and <1 cups/day, ≥1 and <2 cups/day, and ≥2 cups/day. Over a median follow-up of 11.6 years for MASLD and 15.7 years for ALF, coffee consumption did not significantly affect the incidence of MASLD, with 2298 new cases observed. However, a notable inverse association was found with ALF risk in patients with MASLD among those consuming coffee ≥2 cups/day (adjusted HR 0.57, 95% CI: 0.37–0.90, p = 0.014), especially among those consuming coffee ≥2 and <3 cups/day (adjusted HR 0.51, 95% CI: 0.30–0.89, p = 0.018). This suggests a potential hepatoprotective effect of coffee, especially in preventing the progression of liver fibrosis in patients with MASLD. These findings propose that coffee consumption could be a simple and effective approach to mitigate the risk of ALF in individuals with MASLD.
Central Nervous System Tuberculosis in a Murine Model: Neurotropic Strains or a New Pathway of Infection?
Authors: Rembao-Bojórquez Daniel , Sánchez-Garibay Carlos , Salinas-Lara Citlaltepetl , Marquina-Castillo Brenda , Letechipía-Salcedo Adriana , Castillón-Benavides Omar , Galván-Arzate Sonia , Gómez-López Marcos , Jiménez-Zamudio Luis , Soto-Rojas Luis , Tena-Suck Martha , Nava Porfirio , Fernández-Vargas Omar , Coria-Medrano Adrian , Hernández-Pando Rogelio
Published: December 2023

Tuberculosis (TB) of the central nervous system (CNS) is a lethal and incapacitating disease. Several studies have been performed to understand the mechanism of bacterial arrival to CNS, however, it remains unclear. Although the interaction of the host, the pathogen, and the environment trigger the course of the disease, in TB the characteristics of these factors seem to be more relevant in the genesis of the clinical features of each patient. We previously tested three mycobacterial clinical isolates with distinctive genotypes obtained from the cerebrospinal fluid of patients with meningeal TB and showed that these strains disseminated extensively to the brain after intratracheal inoculation and pulmonary infection in BALB/c mice. In this present study, BALB/c mice were infected through the intranasal route. One of these strains reaches the olfactory bulb at the early stage of the infection and infects the brain before the lungs, but the histological study of the nasal mucosa did not show any alteration. This observation suggests that some mycobacteria strains can arrive directly at the brain, apparently toward the olfactory nerve after infecting the nasal mucosa, and guides us to study in more detail during mycobacteria infection the nasal mucosa, the associated connective tissue, and nervous structures of the cribriform plate, which connect the nasal cavity with the olfactory bulb.
Future Prospects, Approaches, and the Government’s Role in the Development of a Hepatitis C Virus Vaccine
Authors: Tabll Ashraf A. , Sohrab Sayed S. , Ali Ahmed A. , Petrovic Ana , Steiner Srdarevic Sabina , Siber Stjepan , Glasnovic Marija , Smolic Robert , Smolic Martina
Published: December 2023

Developing a safe and effective vaccine against the hepatitis C virus (HCV) remains a top priority for global health. Despite recent advances in antiviral therapies, the high cost and limited accessibility of these treatments impede their widespread application, particularly in resource-limited settings. Therefore, the development of the HCV vaccine remains a necessity. This review article analyzes the current technologies, future prospects, strategies, HCV genomic targets, and the governmental role in HCV vaccine development. We discuss the current epidemiological landscape of HCV infection and the potential of HCV structural and non-structural protein antigens as vaccine targets. In addition, the involvement of government agencies and policymakers in supporting and facilitating the development of HCV vaccines is emphasized. We explore how vaccine development regulatory channels and frameworks affect research goals, funding, and public health policy. The significance of international and public-private partnerships in accelerating the development of an HCV vaccine is examined. Finally, the future directions for developing an HCV vaccine are discussed. In conclusion, the review highlights the urgent need for a preventive vaccine to fight the global HCV disease and the significance of collaborative efforts between scientists, politicians, and public health organizations to reach this important public health goal.
A Novel Inhibitor against the Biofilms of Non-Tuberculous Mycobacteria
Authors: Kaur Parvinder , Krishnamurthy Ramya Vadageri , Shandil Radha Krishan , Mohan Rahul , Narayanan Shridhar
Published: December 2023

Non-tuberculous Mycobacteria (NTM), previously classified as environmental microbes, have emerged as opportunistic pathogens causing pulmonary infections in immunocompromised hosts. The formation of the biofilm empowers NTM pathogens to escape from the immune response and antibiotic action, leading to treatment failures. NF1001 is a novel thiopeptide antibiotic first-in-class compound with potent activity against planktonic/replicating and biofilm forms of various NTM species. It is potent against both drug-sensitive and -resistant NTM. It has demonstrated a concentration-dependent killing of replicating and intracellularly growing NTM, and has inhibited and reduced the viability of NTM in biofilms. Combination studies using standard-of-care (SoC) drugs for NTM exhibited synergetic/additive effects, but no antagonism against both planktonic and biofilm populations of Mycobacterium abscessus and Mycobacterium avium. In summary, the activity of NF1001 alone or in combination with SoC drugs projects NF1001 as a promising candidate for the treatment of difficult-to-treat NTM pulmonary diseases (NTM-PD) and cystic fibrosis (CF) in patients.
Therapeutic Effects of IL-1RA against Acute Bacterial Infections, including Antibiotic-Resistant Strains
Authors: Ambite Ines , Tran Thi Hien , Butler Daniel S. C. , Cavalera Michele , Wan Murphy Lam Yim , Ahmadi Shahram , Svanborg Catharina
Published: December 2023

Innate immunity is essential for the anti-microbial defense, but excessive immune activation may cause severe disease. In this study, immunotherapy was shown to prevent excessive innate immune activation and restore the anti-bacterial defense. E. coli-infected Asc−/− mice develop severe acute cystitis, defined by IL-1 hyper-activation, high bacterial counts, and extensive tissue pathology. Here, the interleukin-1 receptor antagonist (IL-1RA), which inhibits IL-1 hyper-activation in acute cystitis, was identified as a more potent inhibitor of inflammation and NK1R- and substance P-dependent pain than cefotaxime. Furthermore, IL-1RA treatment inhibited the excessive innate immune activation in the kidneys of infected Irf3−/− mice and restored tissue integrity. Unexpectedly, IL-1RA also accelerated bacterial clearance from infected bladders and kidneys, including antibiotic-resistant E. coli, where cefotaxime treatment was inefficient. The results suggest that by targeting the IL-1 response, control of the innate immune response to infection may be regained, with highly favorable treatment outcomes, including infections caused by antibiotic-resistant strains.
A Narrative Review: Analysis of Supplemental Parenteral Nutrition in Adults at the End of Life
Authors: Rivas García Francisco , Martínez Rafael , Camarasa Felipe , Cerdá Joan , Messeguer Fuensanta , Gallardo Margarita
Published: December 2023

“End of life” is a stage defined by the existence of an irreversible prognosis that ends with a person’s death. One of the aspects of interest regarding end of life focuses on parenteral nutrition, which is usually administered in order to avoid malnutrition and associated complications. However, parenteral nutrition can be adapted to specific circumstances and evolve in its functionality through supplementation with certain nutrients that can have a beneficial effect. This narrative review aims to carry out a situation analysis of the role that could be adopted by supplemental parenteral nutrition in attenuating alterations typical of end of life and potential improvement in quality of life.
Natural Biopolymer-Based Delivery of CRISPR/Cas9 for Cancer Treatment
Authors: Lin Meng , Wang Xueyan
Published: December 2023

Over the last decade, the clustered, regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become the most promising gene editing tool and is broadly utilized to manipulate the gene for disease treatment, especially for cancer, which involves multiple genetic alterations. Typically, CRISPR/Cas9 machinery is delivered in one of three forms: DNA, mRNA, or ribonucleoprotein. However, the lack of efficient delivery systems for these macromolecules confined the clinical breakthrough of this technique. Therefore, a variety of nanomaterials have been fabricated to improve the stability and delivery efficiency of the CRISPR/Cas9 system. In this context, the natural biopolymer-based carrier is a particularly promising platform for CRISPR/Cas9 delivery due to its great stability, low toxicity, excellent biocompatibility, and biodegradability. Here, we focus on the advances of natural biopolymer-based materials for CRISPR/Cas9 delivery in the cancer field and discuss the challenges for their clinical translation.
Assessing Trans-Inhibition of OATP1B1 and OATP1B3 by Calcineurin and/or PPIase Inhibitors and Global Identification of OATP1B1/3-Associated Proteins
Authors: Powell John T. , Kayesh Ruhul , Ballesteros-Perez Alexandra , Alam Khondoker , Niyonshuti Pascaline , Soderblom Erik J. , Ding Kai , Xu Chao , Yue Wei
Published: December 2023

Organic anion transporting polypeptide (OATP) 1B1 and OATP1B3 are key determinants of drug–drug interactions (DDIs). Various drugs including the calcineurin inhibitor (CNI) cyclosporine A (CsA) exert preincubation-induced trans-inhibitory effects upon OATP1B1 and/or OATP1B3 (abbreviated as OATP1B1/3) by unknown mechanism(s). OATP1B1/3 are phosphoproteins; calcineurin, which dephosphorylates and regulates numerous phosphoproteins, has not previously been investigated in the context of preincubation-induced trans-inhibition of OATP1B1/3. Herein, we compare the trans-inhibitory effects exerted on OATP1B1 and OATP1B3 by CsA, the non-analogous CNI tacrolimus, and the non-CNI CsA analogue SCY-635 in transporter-overexpressing human embryonic kidney (HEK) 293 stable cell lines. Preincubation (10–60 min) with tacrolimus (1–10 µM) rapidly and significantly reduces OATP1B1- and OATP1B3-mediated transport up to 0.18 ± 0.03- and 0.20 ± 0.02-fold compared to the control, respectively. Both CsA and SCY-635 can trans-inhibit OATP1B1, with the inhibitory effects progressively increasing over a 60 min preincubation time. At each equivalent preincubation time, CsA has greater trans-inhibitory effects toward OATP1B1 than SCY-635. Preincubation with SCY-635 for 60 min yielded IC50 of 2.2 ± 1.4 µM against OATP1B1, which is ~18 fold greater than that of CsA (0.12 ± 0.04 µM). Furthermore, a proteomics-based screening for protein interactors was used to examine possible proteins and processes contributing to OATP1B1/3 regulation and preincubation-induced inhibition by CNIs and other drugs. A total of 861 and 357 proteins were identified as specifically associated with OATP1B1 and OATP1B3, respectively, including various protein kinases, ubiquitin-related enzymes, the tacrolimus (FK506)-binding proteins FKBP5 and FKBP8, and several known regulatory targets of calcineurin. The current study reports several novel findings that expand our understanding of impaired OATP1B1/3 function; these include preincubation-induced trans-inhibition of OATP1B1/3 by the CNI tacrolimus, greater preincubation-induced inhibition by CsA compared to its non-CNI analogue SCY-635, and association of OATP1B1/3 with various proteins relevant to established and candidate OATP1B1/3 regulatory processes.
Destabilization of Indomethacin-Paracetamol Co-Amorphous Systems by Mechanical Stress
Authors: Di Rong , Rades Thomas , Grohganz Holger
Published: December 2023

Using co-amorphous systems (CAMS) has shown promise in addressing the challenges associated with poorly water-soluble drugs. Quench-cooling is a commonly used CAMS preparation method, often followed by grinding or milling to achieve a fine powder that is suitable for subsequent characterization or further down-stream manufacturing. However, the impact of mechanical stress applied to CAMS has received little attention. In this study, the influence of mechanical stress on indomethacin—paracetamol CAMS was investigated. The investigation involved thermal analysis and solid-state characterization across various CAMS mixing ratios and levels of mechanical stress. The study revealed a negative effect of mechanical stress on stability, particularly on the excess components in CAMS. Higher levels of mechanical stress were observed to induce phase separation or recrystallization. Notably, samples at the optimal mixing ratio demonstrated greater resistance to the destabilization caused by mechanical stress. These results showed the significance of careful consideration of processing methods during formulation and the significance of optimizing mixing ratios in CAMS.
Mark Burgin’s Contribution to the Foundation of Mathematics
Authors: Lev Felix M.
Published: December 2023

In this paper, I attempt to describe Mark Burgin’s results in non-Diophantine mathematics, which are important for the foundation of mathematics and its application in quantum field theory. In particular, the elimination of divergences in quantum electrodynamics is described.
Quantum Dissipative Adaptation with Cascaded Photons
Authors: Ganascini Thiago , Werlang Thiago , Valente Daniel
Published: December 2023

Classical dissipative adaptation is a hypothetical non-equilibrium thermodynamic principle of self-organization in driven matter, and it relates transition probabilities with the non-equilibrium work performed by an external drive on dissipative matter. Recently, the dissipative adaptation hypothesis was extended to a quantum regime with a theoretical model where only one single-photon pulse drives each atom of an ensemble. Here, we further generalize that quantum model by analytically showing that N cascaded single-photon pulses driving each atom still fulfill a quantum dissipative adaptation. Interestingly, we find that the level of self-organization achieved with two pulses can be matched with a single effective pulse only up to a threshold, above which the presence of more photons provides unparalleled degrees of self-organization.
Enhanced Bolometric Detection of THz Signals by a Resonant Structure for Inclined Radiation Incidence
Authors: Nikiforova Polina , Bogatskaya Anna , Popov Alexander
Published: December 2023

In this work, we consider the possibility of enhancing terahertz bolometric detection efficiency using resonant structures in the case of an inclined incidence of radiation. The structures are made of a sequence of doped and undoped semiconductors, including epsilon-near-zero areas. Undoped regions act as electromagnetic resonators, thus ensuring resonant signal penetration through the opaque (doped) regions of the structure. A set of epsilon-near-zero areas can ensure substantial enhancements to the electric field in the material. In the doped regions, absorption occurs. The structure described above can provide efficient resonant energy absorption for a wide range of angles of incidence. The numerical calculations based on the solution of the Helmholtz equation have shown that the studied resonant structures ensure the absorption of up to 50% of the incident radiation energy for a 60-degree incidence.
Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars
Authors: Bárta Jan , Roudnický Pavel , Jarošová Markéta , Zdráhal Zbyněk , Stupková Adéla , Bártová Veronika , Krejčová Zlatuše , Kyselka Jan , Filip Vladimír , Říha Václav , Lorenc František , Bedrníček Jan , Smetana Pavel
Published: December 2023

As a source of nutritionally important components, hemp seeds are often dehulled for consumption and food applications by removing the hard hulls, which increases their nutritional value. The hulls thus become waste, although they may contain valuable protein items, about which there is a lack of information. The present work is therefore aimed at evaluating the proteome of hemp (Cannabis sativa L.) at the whole-seed, dehulled seed, and hull levels. The evaluation was performed on two cultivars, Santhica 27 and Uso-31, using LC-MS/MS analysis. In total, 2833 protein groups (PGs) were identified, and their relative abundances were determined. A set of 88 PGs whose abundance exceeded 1000 ppm (MP88 set) was considered for further evaluation. The PGs of the MP88 set were divided into ten protein classes. Seed storage proteins were found to be the most abundant protein class: the averages of the cultivars were 65.5%, 71.3%, and 57.5% for whole seeds, dehulled seeds, and hulls, respectively. In particular, 11S globulins representing edestin (three PGs) were found, followed by 7S vicilin-like proteins (four PGs) and 2S albumins (two PGs). The storage 11S globulins in Santhica 27 and Uso-31 were found to have a higher relative abundance in the dehulled seed proteome (summing to 58.6 and 63.2%) than in the hull proteome (50.5 and 54%), respectively. The second most abundant class of proteins was oleosins, which are part of oil-body membranes. PGs belonging to metabolic proteins (e.g., energy metabolism, nucleic acid metabolism, and protein synthesis) and proteins related to the defence and stress responses were more abundant in the hulls than in the dehulled seeds. The hulls can, therefore, be an essential source of proteins, especially for medical and biotechnological applications. Proteomic analysis has proven to be a valuable tool for studying differences in the relative abundance of proteins between dehulled hemp seeds and their hulls among different cultivars.
The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome
Authors: Sokolova Ekaterina Alexeevna , Mishukova Olga Viktorovna , Hlistun Inna Viktorovna , Tromenschleger Irina Nikolaevna , Tikunov Artem Yurievich , Manakhov Andrey Dmitrievich , Rogaev Evgeny Ivanovich , Savenkov Oleg Alexandrovich , Buyanova Maria Dmitrievna , Ivanov Ilya Vladimirovich , Smirnova Natalya Valentinovna , Voronina Elena Nikolaevna
Published: December 2023

The amalgamation of mineral and targeted bacterial preparations represents a new generation of agricultural technology. Inoculation with combined preparations of microorganisms is more effective than inoculation with a single microorganism in stimulating plant growth by providing a more balanced diet for various crops. In this work, the effect of inoculation of 20 consortium variants on the yield indicators of three crops (wheat, buckwheat, corn) and the soil microbiome in the open field was investigated. The soil microbiome was defined by 16S rRNA sequences through NGS. The species richness of the soil microbial community (alpha diversity) was similar for all studied samples. A beta-diversity analysis revealed that the microbial diversity of three soil samples (C.bw, F.bw and Soil.bw) differed significantly from all others. At the phylum level, the number of Acidobacteriota and Firmicutes in these samples was increased. For the combination “Consortium C (Rothia endophytic GMG9 and Azotobacter chroococcum GMG39)—buckwheat”, a systemic positive improvement in all growth and yield indicators was observed. The soil of the site where buckwheat grew, inoculated by Consortium C, contained significantly more available phosphorus than all other soil samples. Such results can be explained both by the direct action of a consortium of phosphate-immobilizing and nitrogen-fixing bacteria and acidification of the medium due to an increase in phylum Acidobacteriota bacteria in the soil.
Anthocyanin Production from Plant Cell and Organ Cultures In Vitro
Authors: Murthy Hosakatte Niranjana , Joseph Kadanthottu Sebastian , Paek Kee Yoeup , Park So-Young
Published: December 2023

Anthocyanins are water-soluble pigments found in plants. They exist in various colors, including red, purple, and blue, and are utilized as natural colorants in the food and cosmetics industries. The pharmaceutical industry uses anthocyanins as therapeutic compounds because they have several medicinal qualities, including anti-obesity, anti-cancer, antidiabetic, neuroprotective, and cardioprotective effects. Anthocyanins are conventionally procured from colored fruits and vegetables and are utilized in the food, pharmaceutical, and cosmetic industries. However, the composition and concentration of anthocyanins from natural sources vary quantitively and qualitatively; therefore, plant cell and organ cultures have been explored for many decades to understand the production of these valuable compounds. A great deal of research has been carried out on plant cell cultures using varied methods, such as the selection of suitable cell lines, medium optimization, optimization culture conditions, precursor feeding, and elicitation for the production of anthocyanin pigments. In addition, metabolic engineering technologies have been applied for the hyperaccumulation of these compounds in varied plants, including tobacco and arabidopsis. In this review, we describe various strategies applied in plant cell and organ cultures for the production of anthocyanins.
Impact of Sodium Nitroprusside on the Photosynthetic Performance of Maize and Sorghum
Authors: Rashkov Georgi D. , Stefanov Martin A. , Yotsova Ekaterina K. , Borisova Preslava B. , Dobrikova Anelia G. , Apostolova Emilia L.
Published: December 2023

Nitric oxide (NO) is an important molecule in regulating plant growth, development and photosynthetic performance. This study investigates the impact of varying concentrations (0–300 µM) of sodium nitroprusside (SNP, a donor of NO) on the functions of the photosynthetic apparatus in sorghum (Sorghum bicolor L. Albanus) and maize (Zea mays L. Kerala) under physiological conditions. Analysis of the chlorophyll fluorescence signals (using PAM and the JIP-test) revealed an increased amount of open PSII reaction centers (qP increased), but it did not affect the number of active reaction centers per PSII antenna chlorophyll (RC/ABS). In addition, the smaller SNP concentrations (up to 150 μM) alleviated the interaction of QA with plastoquine in maize, while at 300 μM it predominates the electron recombination on QAQB−, with the oxidized S2 (or S3) states of oxygen evolving in complex ways in both studied plant species. At the same time, SNP application stimulated the electron flux-reducing end electron acceptors at the PSI acceptor side per reaction center (REo/RC increased up to 26%) and the probability of their reduction (φRo increased up to 20%). An increase in MDA (by about 30%) and H2O2 contents was registered only at the highest SNP concentration (300 µM). At this concentration, SNP differentially affected the amount of P700+ in studied plant species, i.e., it increased (by 10%) in maize but decreased (by 16%) in sorghum. The effects of SNP on the functions of the photosynthetic apparatus were accompanied by an increase in carotenoid content in both studied plants. Additionally, data revealed that SNP-induced changes in the photosynthetic apparatus differed between maize and sorghum, suggesting species specificity for SNP’s impact on plants.
Characteristics of Carbon and Kevlar Fibres, Their Composites and Structural Applications in Civil Engineering—A Review
Authors: Ursache Ștefania , Cerbu Camelia , Hadăr Anton
Published: December 2023

Kevlar and carbon fibres and fabrics have won a leading place in the structure market, although such materials are not cheap, and are increasingly used for reinforcing and strengthening structural elements in the civil engineering, automotive, aerospace and military industries, due to their superior mechanical properties, especially in terms of strength. The mechanical characteristics of such composite materials must be known in order to numerically simulate the mechanical behaviour of such structures in terms of the distribution of stresses and strains. It has also become a necessity to understand the effects of reinforcement with both types of fibres (carbon fibres and Kevlar fibres) on the mechanical properties, especially on the impact properties of such composites. This review aims to expose the main advantages and disadvantages of the hybridization of carbon and Kevlar fibres. For this reason, an overview is presented concerning the main characteristics (tensile strength, flexural strength, impact strength, coefficient of thermal expansion and so on) for carbon and Kevlar fibres and also for hybrid Kevlar–carbon composite materials to aid in the design of such hybrid composite materials. Finally, some civil construction rehabilitation and consolidation applications of the composites reinforced with carbon fibre, Kevlar fibre or with hybrid Kevlar–carbon fabrics are highlighted in the last part of the paper.
Amide-Containing Bottlebrushes via Continuous-Flow Photoiniferter Reversible Addition–Fragmentation Chain Transfer Polymerization: Micellization Behavior
Authors: Sivokhin Alexey , Orekhov Dmitry , Kazantsev Oleg , Otopkova Ksenia , Sivokhina Olga , Chuzhaykin Ilya , Ovchinnikov Alexey , Zamyshlyayeva Olga , Pavlova Irina , Ozhogina Olga , Chubenko Maria
Published: December 2023

Herein, a series of ternary amphiphilic amide-containing bottlebrushes were synthesized by photoiniferter (PI-RAFT) polymerization of macromonomers in continuous-flow mode using trithiocarbonate as a chain transfer agent. Visible light-mediated polymerization of macromonomers under mild conditions enabled the preparation of thermoresponsive copolymers with low dispersity and high yields in a very short time, which is not typical for the classical reversible addition–fragmentation chain transfer process. Methoxy oligo(ethylene glycol) methacrylate and alkoxy(C12–C14) oligo(ethylene glycol) methacrylate were used as the basic monomers providing amphiphilic and thermoresponsive properties. The study investigated how modifying comonomers, acrylamide (AAm), methacrylamide (MAAm), and N-methylacrylamide (-MeAAm) affect the features of bottlebrush micelle formation, their critical micelle concentration, and loading capacity for pyrene, a hydrophobic drug model. The results showed that the process is scalable and can produce tens of grams of pure copolymer per day. The unmodified copolymer formed unimolecular micelles at temperatures below the LCST in aqueous solutions, as revealed by DLS and SLS data. The incorporation of AAm, MAAm, and N-MeAAm units resulted in an increase in micelle aggregation numbers. The resulting bottlebrushes formed uni- or bimolecular micelles at extremely low concentrations. These micelles possess a high capacity for loading pyrene, making them a promising choice for targeted drug delivery.
3D Oleophilic Sorbent Films Based on Recycled Low-Density Polyethylene
Authors: Saleem Junaid , Moghal Zubair Khalid Baig , McKay Gordon
Published: December 2023

Recycling low-end, one-time-use plastics—such as low-density polyethylene (LDPE)—is of paramount importance to combat plastic pollution and promote sustainability in the modern green economy. This study valorizes LDPE waste by transforming it into 3D oleophilic swellable thin films through a process involving dissolution, phase separation, and extraction. These films are subsequently layered using a customized polypropylene (PP) based nonwoven fabric separator and securely sealed in a zigzag pattern. The zigzag-shaped seal enhances the adhesion of pollutants to the sorbent by providing wire curvatures that increase retention time and uptake capacity. As a result, the sorbent exhibits impressive oil uptake capacities, with immediate and equilibrium values of 120 g/g and 85 g/g, respectively. Notably, the as-prepared sorbent demonstrates low water retention and high selectivity for oil, outperforming commercially available oil sorbents. The unique design involving a 3D-film structure, superposed films, and a zigzag-shaped seal offers a sustainable and value-added solution to the issues of LDPE waste and oil spills on water surfaces.
Catalytic Ring-Opening Polymerisation of Cyclic Ethylene Carbonate: Importance of Elementary Steps for Determining Polymer Properties Revealed via DFT-MTD Simulations Validated Using Kinetic Measurements
Authors: Brüggemann Daniel , Machat Martin R. , Schomäcker Reinhard , Heshmat Mojgan
Published: December 2023

The production of CO2-containing polymers is still very demanding in terms of controlling the synthesis of products with pre-defined CO2 content and molecular weight. An elegant way of synthesising these polymers is via CO2-containing building blocks, such as cyclic ethylene carbonate (cEC), via catalytic ring-opening polymerisation. However, to date, the mechanism of this reaction and control parameters have not been elucidated. In this work, using DFT-metadynamics simulations for exploiting the potential of the polymerisation process, we aim to shed more light on the mechanisms of the interaction between catalysts (in particular, the catalysts K3VO4, K3PO4, and Na2SnO3) and the cEC monomer in the propagation step of the polymeric chain and the occurring CO2 release. Confirming the simulation results via subsequent kinetics measurements indicates that, depending on the catalyst’s characteristics, it can be attached reversibly to the polymeric chain during polymerisation, resulting in a defined lifetime of the activated polymer chain. The second anionic oxygen of the catalyst can promote the catalyst’s transfer to another electrophilic cEC monomer, terminating the growth of the first chain and initiating the propagation of the new polymer chain. This transfer reaction is an essential step in controlling the molecular weight of the products.
Development of an Innovative Lightweight Composite Material with Thermal Insulation Properties Based on Cardoon and Polyurethane
Authors: Fernandes Raquel A. , Ferreira Nuno , Lopes Sandro , Santos Jorge , Bento Pereira Nelson , Ferreira Nuno Oliveira , Nunes Lina , Martins Jorge M. , Carvalho Luisa H.
Published: December 2023

The search for innovative and sustainable solutions to improve the energy efficiency of the construction industry has been a hot topic for researchers due to the tremendous impact of insulator materials in the thermal comfort of buildings. In the present work, an innovative lightweight composite material with thermal insulation properties was developed, for the first time, by using cardoon particles and polyurethane. The formulation of the composite material was optimized in terms of cardoon fraction and the polyol/isocyanate ratio, to achieve the best compromise between internal bond (IB) strength and thickness swelling (TS). The best performing composite was PU75-CP45, with 45 wt% of cardoon particles and 75% of isocyanate, achieving an IB of 0.41 MPa and a TS of 5.3%. Regarding insulation properties, the PU75-CP45 composite material exhibits a promising performance when compared to conventional construction industry materials by tuning its thickness. Additionally, the composite material presented very low emissions of volatile organic compounds and formaldehyde (bellow to legislation levels) and high resistance to biological degradation.
Inhibition of Pro-Fibrotic Molecules Expression in Idiopathic Pulmonary Fibrosis—Derived Lung Fibroblasts by Lactose-Modified Hyaluronic Acid Compounds
Authors: Donato Alice , Di Stefano Antonino , Freato Nadia , Bertocchi Laura , Brun Paola
Published: December 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic inflammatory and fibrotic pathological condition with undefined effective therapies and a poor prognosis, partly due to the lack of specific and effective therapies. Galectin 3 (Gal-3), a pro-fibrotic ß-galactoside binding lectin, was upregulated in the early stages of the pathology, suggesting that it may be considered a marker of active fibrosis. In the present in vitro study, we use Hylach®, a lactose-modified hyaluronic acid able to bind Gal-3, to prevent the activation of lung myofibroblast and the consequent excessive ECM protein cell expression. Primary human pulmonary fibroblasts obtained from normal and IPF subjects activated with TGF-β were used, and changes in cell viability, fibrotic components, and pro-inflammatory mediator expression at both gene and protein levels were analyzed. Hylach compounds with a lactosylation degree of about 10% and 30% (Hylach1 and Hylach 2), administrated to TGF-β—stimulated lung fibroblast cultures, significantly downregulated α-smooth muscle actin (α-SMA) gene expression and decreased collagen type I, collagen type III, elastin, fibronectin gene and protein expression to near baseline values. This anti-fibrotic activity is accompanied by a strong anti-inflammatory effect and by a downregulation of the gene expression of Smad2 for both Hylachs in comparison to the native HA. In conclusion, the Gal-3 binding molecules Hylachs attenuated inflammation and TGF-β—induced over-expression of α-SMA and ECM protein expression by primary human lung fibroblasts, providing a new direction for the treatment of pulmonary fibrotic diseases.
Low-Carbon Economic Dispatch of Virtual Power Plant Considering Hydrogen Energy Storage and Tiered Carbon Trading in Multiple Scenarios
Authors: Xie Tuo , Wang Qi , Zhang Gang , Zhang Kaoshe , Li Hua
Published: December 2023

Reducing carbon emissions and increasing the integration of new energy sources are key steps towards achieving sustainable development. Virtual power plants (VPPs) play a significant role in enhancing grid security and promoting the transition to clean, low-carbon energy. The core equipment of the VPP, the CHP unit, utilizes a thermal engine or power station to generate electricity and useful heat simultaneously. However, the intermittent and volatile nature of renewable energy sources, as well as the “heat-driven power generation” mode of combined heat and power (CHP) units, presents contradictions that severely affect their peak-shifting capability and lead to high carbon emissions. To address these issues, a novel VPP is established by integrating traditional power plants with carbon capture and hydrogen energy storage. This approach utilizes a “hydrogen energy storage–electric boiler” decoupling method to address the operational mode of CHP, strengthens the coupling relationship between electric and thermal hydrogen loads, and considers a tiered carbon-trading mechanism. With the net profit of the VPP as the optimization objective, the model balances economic and environmental considerations and establishes a low-carbon economic dispatch model for the VPP. A genetic algorithm is employed for solving, and three different dispatch strategies are set for simulation in three distinct seasonal scenarios. The comprehensive comparative analysis of the dispatch results reveals a reduction in carbon emissions and an increase in net profit to varying degrees across all three seasons. Overall, the proposed dispatch strategy demonstrates the ability to enhance the new energy-integration capacity and total revenue of a VPP while simultaneously achieving the goal of reducing carbon emissions.