bluuebunny/crossref_metadata_2025 huggingface dataset:
NK cells in peripheral blood carry trogocytosed tumor antigens from solid cancer cells
Authors: Campos-Mora Mauricio , Jacot William , Garcin Genevieve , Depondt Marie-Lise , Constantinides Michael , Alexia Catherine , Villalba Martin
Published: August 2023

The innate immune lymphocyte lineage natural killer (NK) cell infiltrates tumor environment where it can recognize and eliminate tumor cells. NK cell tumor infiltration is linked to patient prognosis. However, it is unknown if some of these antitumor NK cells leave the tumor environment. In blood-borne cancers, NK cells that have interacted with leukemic cells are recognized by the co-expression of two CD45 isoforms (CD45RARO cells) and/or the plasma membrane presence of tumor antigens (Ag), which NK cells acquire by trogocytosis. We evaluated solid tumor Ag uptake by trogocytosis on NK cells by performing co-cultures in vitro. We analyzed NK population subsets by unsupervised dimensional reduction techniques in blood samples from breast tumor (BC) patients and healthy donors (HD). We confirmed that NK cells perform trogocytosis from solid cancer cells in vitro. The extent of trogocytosis depends on the target cell and the antigen, but not on the amount of Ag expressed by the target cell or the sensitivity to NK cell killing. We identified by FlowSOM (Self-Organizing Maps) several NK cell clusters differentially abundant between BC patients and HD, including anti-tumor NK subsets with phenotype CD45RARO+CD107a+. These analyses showed that bona-fide NK cells that have degranulated were increased in patients and, additionally, these NK cells exhibit trogocytosis of solid tumor Ag on their surface. However, the frequency of NK cells that have trogocytosed is very low and much lower than that found in hematological cancer patients, suggesting that the number of NK cells that exit the tumor environment is scarce. To our knowledge, this is the first report describing the presence of solid tumor markers on circulating NK subsets from breast tumor patients. This NK cell immune profiling could lead to generate novel strategies to complement established therapies for BC patients or to the use of peripheral blood NK cells in the theranostic of solid cancer patients after treatment.
The role of p53 in anti-tumor immunity and response to immunotherapy
Authors: Carlsen Lindsey , Zhang Shengliang , Tian Xiaobing , De La Cruz Arielle , George Andrew , Arnoff Taylor E. , El-Deiry Wafik S.
Published: August 2023

p53 is a transcription factor that regulates the expression of genes involved in tumor suppression. p53 mutations mediate tumorigenesis and occur in approximately 50% of human cancers. p53 regulates hundreds of target genes that induce various cell fates including apoptosis, cell cycle arrest, and DNA damage repair. p53 also plays an important role in anti-tumor immunity by regulating TRAIL, DR5, TLRs, Fas, PKR, ULBP1/2, and CCL2; T-cell inhibitory ligand PD-L1; pro-inflammatory cytokines; immune cell activation state; and antigen presentation. Genetic alteration of p53 can contribute to immune evasion by influencing immune cell recruitment to the tumor, cytokine secretion in the TME, and inflammatory signaling pathways. In some contexts, p53 mutations increase neoantigen load which improves response to immune checkpoint inhibition. Therapeutic restoration of mutated p53 can restore anti-cancer immune cell infiltration and ameliorate pro-tumor signaling to induce tumor regression. Indeed, there is clinical evidence to suggest that restoring p53 can induce an anti-cancer immune response in immunologically cold tumors. Clinical trials investigating the combination of p53-restoring compounds or p53-based vaccines with immunotherapy have demonstrated anti-tumor immune activation and tumor regression with heterogeneity across cancer type. In this Review, we discuss the impact of wild-type and mutant p53 on the anti-tumor immune response, outline clinical progress as far as activating p53 to induce an immune response across a variety of cancer types, and highlight open questions limiting effective clinical translation.
Identification of ferroptosis-related genes as potential diagnostic biomarkers for diabetic nephropathy based on bioinformatics
Authors: Guo Binbin , Li Minhui , Wu Peipei , Chen Yan
Published: August 2023

Objective: This study investigated to probe ferroptosis-related diagnostic biomarkers and underlying molecular mechanisms in Diabetic nephropathy (DN).

Methods: GSE30122 and GSE1009 from GEO database were used as training and verification sets, respectively, to screen differentially expressed ferroptosis-related genes (FRGs). These genes were further analyzed using GO, KEGG, and GSEA methods, and screened with PPI, LASSO, and SVM-RFE to identify ferroptosis-related diagnostic biomarkers for DN. A diagnostic model was established using the Glm function and verified with ROC curve. The relationship between these biomarkers and immune cell was analyzed, and qRT-PCR and Western blot were used to detect the expression of these biomarkers in kidney tissues and identify the effect of TP53 on DN development.

Results: Fifty one differentially expressed FRGs were enriched in bioprocesses such as p53 signaling pathway, oxidative stress and chemical stress response, and mTOR signaling pathway. TP53, RB1, NF2, RRM2, PRDX1, and CDC25A were identified as ferroptosis-related diagnostic biomarkers for DN. TP53 showed the most differential expression. ROC analysis showed that AUC values of TP53, RB1, NF2, RRM2, PRDX1, and CDC25A were 0.751, 0.705, 0.725, 0.882, 0.691, and 0.675, respectively. The AUC value of DN diagnosis model was 0.939 in training set and 1.000 in verification set. qRT-PCR results confirmed significant differences in these six biomarkers between DN and normal kidney tissue (p < 0.05), and correlation analysis showed that five biomarkers were significantly correlated with infiltrating immune cells (p < 0.05). Furthermore, western blots showed that TP53 promotes apoptosis through PI3K-AKT signaling in DN.

Conclusion:TP53, RB1, NF2, RRM2, PRDX1, and CDC25A have potential as diagnostic biomarkers for DN. The diagnostic model containing the above six biomarkers performs well in the diagnosis of DN. Five of the six biomarkers are strongly associated with several infiltrating immune cells. TP53 may play an essential role in the development of DN.
Uncovering the role of Rad51 in homologous recombination-mediated antigenic diversification in the human malaria parasite Plasmodium falciparum
Authors: Vydyam Pratap , Roy Nabamita , Bhattacharyya Mrinal Kanti
Published: August 2023

The human malaria parasite Plasmodium falciparum maintains the chronicity of infections through antigenic variation, a well-coordinated immune evasion mechanism. The most prominent molecular determinant of antigenic variation in this parasite includes the members of the var multigene family. Homologous recombination (HR)-mediated genomic rearrangements have been implicated to play a major role in var gene diversification. However, the key molecular factors involved in the generation of diversity at var loci are less known. Here, we tested the hypothesis that PfRad51 could carry out recombination between var genes that are not homologous but homeologous in nature. We employed the whole-genome sequencing (WGS) approach to investigate recombination events among var sequences over 100 generations and compared the rate of sequence rearrangement at the var loci in both PfRad51-proficient and -deficient parasite lines. This brief report provides evidence that the loss of the key recombinase function renders the parasite with inefficient HR and results in fewer recombination events among the var sequences, thereby impacting the diversification of the var gene repertoire.
The effect of accompanying anxiety and depression on patients with different vestibular syndromes
Authors: Feng Shuai , Zang Jian
Published: August 2023

Objective

This study aims to investigate the situation of vertigo disorder combined with anxiety and depression in patients with different types of vestibular syndrome.

Methods

A total of 330 patients with vertigo in otolaryngology outpatient department were selected, and clinical information such as age, gender, and scores of Dizziness handicap inventory (DHI), Generalized anxiety disorder-7 (GAD-7), and Patient Health Questionnaire-9 (PHQ-9) were collected. Analyzed the differences among acute vestibular syndrome (AVS), episodic vestibular syndrome (EVS) and chronic vestibular syndrome (CVS) in terms of age, gender, comorbid anxiety and depression, and the multivariate ordered logistic regression analysis was used to evaluate the relationship between the above factors and the degree of vertigo disorder.

Results

The three types of vestibular syndrome had no significant difference in age composition, sex composition, anxiety and depression. There was no significant difference in the probability of anxiety and depression among vertigo patients of different ages and genders. The total score of vertigo disorder and each sub-item score were higher in patients with anxiety and depression. Patients with anxiety mainly manifested in EVS and CVS, while patients with depression mainly manifested in EVS and AVS. The probability of increased vertigo in anxious patients was 4.65 times that of non-anxious patients, and the probability of increased vertigo in depressed patients was 3.49 times that of non-depressed patients. Age and gender had no statistically significant effect on the degree of vertigo. In patients with EVS, anxiety and depression had a significant effect on the degree of vertigo; in patients with CVS, anxiety had a significant effect on the degree of vertigo, but depression had no significant effect.

Conclusion

Age and gender do not significantly affect the degree of vertigo disorder and mental state in various vestibular syndromes. Instead, anxiety and depression are the risk factors for aggravating the degree of vertigo disorder, and manifest differently in each type of vestibular syndrome. Therefore, it is necessary to use a quick scale tool to conduct a standardized screening of the psychological status of patients with vertigo.
Fish consumption and risk of prostate cancer or its mortality: an updated systematic review and dose–response meta-analysis of prospective cohort studies
Authors: Eshaghian Niloofar , Heidarzadeh-Esfahani Neda , Akbari Hakimeh , Askari Gholamreza , Sadeghi Omid
Published: August 2023

Since the release of the last meta-analysis on the association between fish intake and prostate cancer risk, several cohort studies have been published. Moreover, none of the previous meta-analyzes examined the dose–response association between fish intake and prostate cancer. Therefore, the current dose–response meta-analysis was conducted to summarize available findings on the associations of fish intake with the risk of prostate cancer in men. Online databases of PubMed, Scopus, and Web of Science were systematically searched up to September 2022. We included prospective cohort studies that examined the associations of fish intake with the risk of prostate cancer (total, localized, and advanced prostate cancer), its mortality, and cancer progression. Summary relative risks (RR) and 95% confidence intervals (CI) were calculated for the highest versus lowest categories of fish intake using random-effects models. Also, linear and non-linear dose–response analyzes were conducted. In total, 25 prospective cohort studies, recruiting 1,216,474 men, were included in the systematic review, and 22 studies were included in the meta-analysis. During the follow-up periods, ranging from 6 to 33 years, a total of 44,722 cases of prostate cancer were recorded. The comparison between the highest and lowest intakes of total fish revealed the summary RRs of 0.97 (95% CI: 0.86–1.10) for total, 1.01 (95% CI: 0.91–1.13) for advanced, and 0.90 (95% CI: 0.72–1.12) for localized prostate cancer, indicating no significant association. Moreover, the summary RR was 0.55 (95% CI: 0.33–0.92) for prostate cancer mortality and 0.84 (95% CI: 0.65–1.10) for prostate cancer progression, indicating an inverse association between fish intake and prostate cancer mortality. Also, in the dose–response analyzes, each 20 gram/day increase in total fish intake was associated with a 12% lower risk of prostate cancer mortality. Our findings support the protective association between total fish intake and the risk of prostate cancer mortality.
Artificial intelligence-based approaches for the detection and prioritization of genomic mutations in congenital surgical diseases
Authors: Lin Qiongfen , Tam Paul Kwong-Hang , Tang Clara Sze-Man
Published: August 2023

Genetic mutations are critical factors leading to congenital surgical diseases and can be identified through genomic analysis. Early and accurate identification of genetic mutations underlying these conditions is vital for clinical diagnosis and effective treatment. In recent years, artificial intelligence (AI) has been widely applied for analyzing genomic data in various clinical settings, including congenital surgical diseases. This review paper summarizes current state-of-the-art AI-based approaches used in genomic analysis and highlighted some successful applications that deepen our understanding of the etiology of several congenital surgical diseases. We focus on the AI methods designed for the detection of different variant types and the prioritization of deleterious variants located in different genomic regions, aiming to uncover susceptibility genomic mutations contributed to congenital surgical disorders.
Salvianolic acid B attenuates inflammation and prevent pathologic fibrosis by inhibiting CD36-mediated activation of the PI3K-Akt signaling pathway in frozen shoulder
Authors: Yan Yan , Zhou Min , Meng Ke , Zhou Chuanhai , Jia Xiaoyu , Li Xinhao , Cui Dedong , Yu Menglei , Tang Yiyong , Li Ming , Zhang Jinming , Wang Zhuo , Hou Jingyi , Yang Rui
Published: August 2023

Frozen shoulder (FS) is characterized by pain and limited range of motion (ROM). Inflammation and fibrosis are accepted as main pathologic processes associated with the development of FS. However, the intrinsic mechanisms underlying pathologic fibrosis remain unclear. We aimed to elucidate the key molecules involved in pathologic fibrosis and explore new therapeutic targets for FS. Synovial fibroblasts isolated from patient biopsies were identified using immunofluorescence. Western blotting, RT-qPCR, cell adhesion tests, and would-healing assays were used to evaluate the fibrosis-related functions of synovial fibroblasts. Elevated cluster of differentiation 36 (CD36) expression was detected in FS using Western blotting and immunohistochemistry. Salvianolic acid b (SaB) inhibited CD36, blocking synovial fibroblast-induced inflammation and fibrosis. Our RNA-seq data showed that knocking down CD36 dramatically impaired the capacity of synovial fibroblasts for cell adhesion and that the PI3K-Akt signaling pathway may be crucial to the fibrotic process of FS. By up-regulating CD36 and inhibiting the phosphorylation of Akt, we demonstrated that CD36 promotes pathologic fibrosis by activating the PI3k-Akt pathway. Finally, rats treated with SaB had improved ROM and less collagen fiber deposition than the FS model group.

Conclusion: SaB attenuates inflammation and inhibited the CD36-mediated activation of the PI3K-Akt signaling pathway to block pathologic fibrosis of FS in vitro and in vivo models.
Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.
Authors: Lantos Edit , Krämer Reiner , Richert-Pöggeler Katja R. , Maiss Edgar , König Janine , Nothnagel Thomas
Published: August 2023

Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1.
The association of early feeding practices with eating behaviors and maternal indulgent feeding behaviors among Saudi preschoolers
Authors: Mosli Rana H. , Kutbi Hebah A.
Published: August 2023

Evidence from previous work suggest that feeding practices during the first years of life may influence the development of eating behaviors later in childhood. Early feeding practices may also predict later maternal feeding behaviors. Mothers from an Arab/Middle Eastern background may adopt unique early feeding practices. The objective of this study was to examine the association of offering fruit juice and date syrup-milk mixture in a baby bottle during infancy with: 1) Child eating behaviors during preschool years; 2) Maternal indulgent feeding practices during preschool years. Mothers of preschoolers (n = 115) completed questions adopted from the Children’s Eating Behavior Questionnaire (CEBQ), Child Feeding Questionnaire-Arabic (CFQ-A), as well as questions assessing early feeding practices and demographic characteristics. Unadjusted and adjusted logistic regression models were tested to examine the association of early feeding practices with child eating behaviors and maternal indulgent feeding behaviors. Odds ratios (ORs) and respective 95% confidence intervals (CI) were derived. Adjusting for covariates, children who were ever offered fruit juice in a baby bottle as infants were more likely to have high food responsiveness at preschool, compared to children who were not (OR = 2.93, 95% CI = 1.16, 7.42). Mothers who reported ever offering fruit juice in a baby bottle when their children were infants were more likely to adopt greater use of food as a reward when their children were at preschool (OR = 3.25, 95% CI = 1.22, 8.68). Early feeding practices are associated with child eating and maternal feeding behaviors later in childhood. Future longitudinal studies are needed to further establish these associations. Findings from this study can help inform community awareness and education programs to prevent maladaptive feeding practices and promote appropriate feeding strategies throughout childhood.
Evaluating employability in contexts of change: validation of a scale
Authors: González-Navarro Pilar , Córdoba-Iñesta Ana Isabel , Casino-García Ana María , Llinares-Insa Lucía Inmaculada
Published: August 2023

Introduction

Employability is a crucial factor in managing to emerge and changing job demands. This study validates an expanded version of the Employability Appraisal Scale: EAS-60, as an instrument to identify and improve competencies for employability.

Method

The EAS-60 was tested in a cross-sectional study in a Spanish population. An exploratory study was carried out using a sample of 188 workers, and the scale’s structure was analyzed and confirmed in two Confirmatory Factor Analyses using a sample of 527 workers. Finally, reliability and validity were evaluated.

Results

Exploratory and confirmatory analyses provide evidence supporting the multi-dimensional structure. The scale presents good psychometric properties and criteria for interpreting the scores.

Discussion

The EAS-60 is a reliable and valid instrument. It allows Human Resource Managers to offer career plans at work that include specific actions of job socialization, training, improvement of specific skills, etc. Furthermore, employees can increase their employability and develop their professional careers.
Cancer treatment-related financial toxicity in Japan: a scoping review
Authors: Itani Yuki , Obama Kyoko , Fujimori Maiko , Saito Junko , Uchitomi Yosuke
Published: August 2023

Financial toxicity during cancer survival has been studied mainly in the United States; 47–49% of cancer survivors reported financial hardships and 12–63% reported debt owing to treatment costs. Financial toxicity is influenced by each country’s economic status and healthcare system. We aimed to review the evidence on financial toxicity in Japan. A systematic search was performed using PubMed and Ichushi databases. We included English or Japanese peer-reviewed articles that (1) explored the experiences of cancer patients facing financial toxicity due to cancer diagnosis and treatment, (2) were specific to Japan, and (3) focused on the experiences of financial toxicities among cancer patients. Data were extracted focusing on the experiences of patients, families, and healthcare providers. The main themes were synthesized based on a previous study. The search yielded 632 citations from PubMed and 21 from Ichushi, and non-duplicates were identified. Of these, 31 articles were selected for full-text review. Literature was divided into studies describing the following elements: (a) risk factors for financial toxicity, (b) description of financial toxicity, (c) psychological reactions, (d) coping strategies for financial toxicity, and (e) impact on treatment outcomes. Only three studies reported comprehensive financial toxicity scores. Furthermore, treatment costs influenced physicians’ treatment decisions, and patients and their families adopted various strategies to cope with treatment costs. Two studies showed that low current income and younger age were high-risk factors. As for utilization of the support system, approximately 70% of the patients used the high-cost medical expense system, 20% used the sickness benefit system, and 40% used the medical expense deduction system. Many cancer patients in Japan suffer from financial toxicity during cancer survival. One reason for this is that the awareness of the system supporting financial toxicity is insufficient and actual utilization is low. It is necessary to actively encourage patients to ask healthcare providers questions, improve the link between patients and the support system, reconstruct the support system design, and improve the method of publicizing the system.
Adjunctive repetitive transcranial magnetic stimulation for adolescents with first-episode major depressive disorder: a meta-analysis
Authors: Sun Chen-Hui , Mai Jian-Xin , Shi Zhan-Ming , Zheng Wei , Jiang Wen-Long , Li Ze-Zhi , Huang Xing-Bing , Yang Xin-Hu , Zheng Wei
Published: August 2023

Objective

This meta-analysis of randomized clinical trials (RCTs) was conducted to explore the therapeutic effects, tolerability and safety of repetitive transcranial magnetic stimulation (rTMS) as an adjunct treatment in adolescents with first-episode major depressive disorder (FE-MDD).

Methods

RCTs examining the efficacy, tolerability and safety of adjunctive rTMS for adolescents with FE-MDD were included. Data were extracted by three independent authors and synthesized using RevMan 5.3 software with a random effects model.

Results

A total of six RCTs involving 562 adolescents with FE-MDD were included. Adjunctive rTMS was superior in improving depressive symptoms over the control group [standardized mean difference (SMD) = −1.50, 95% confidence interval (CI): −2.16, −0.84; I2 = 89%, p < 0.00001] in adolescents with FE-MDD. A sensitivity analysis and two subgroup analyses also confirmed the significant findings. Adolescents with FE-MDD treated with rTMS had significantly greater response [risk ratio (RR) = 1.35, 95% CI: 1.04, 1.76; I2 = 56%, p = 0.03] and remission (RR = 1.35, 95% CI: 1.03, 1.77; I2 = 0%, p = 0.03) over the control group. All-cause discontinuations were similar between the two groups (RR = 0.79, 95% CI: 0.32, 1.93; I2 = 0%, p = 0.60). No significant differences were found regarding adverse events, including headache, loss of appetite, dizziness and nausea (p = 0.14–0.82). Four out of six RCTs (66.7%), showed that adjunctive rTMS was more efficacious over the control group in improving neurocognitive function (all p < 0.05).

Conclusion

Adjunctive rTMS appears to be a beneficial strategy in improving depressive symptoms and neurocognitive function in adolescents with FE-MDD. Higher quality RCTs with larger sample sizes and longer follow-up periods are warranted in the future.
Global associations of key populations with HIV-1 recombinants: a systematic review, global survey, and individual participant data meta-analysis
Authors: Nchinda Nkazi , Elangovan Ramyiadarsini , Yun Jason , Dickson-Tetteh Leslie , Kirtley Shona , Hemelaar Joris ,   WHO-UNAIDS Network for HIV Isolation and Characterisation
Published: August 2023

Introduction

Global HIV infections due to HIV-1 recombinants are increasing and impede prevention and treatment efforts. Key populations suffer most new HIV infections, but their role in the spread of HIV-1 recombinants is unknown. We conducted a global analysis of the associations between key populations and HIV-1 recombinants.

Methods

We searched PubMed, EMBASE, CINAHL, and Global Health for HIV-1 subtyping studies published from 1/1/1990 to 31/12/2015. Unpublished data was collected through a global survey. We included studies with HIV-1 subtyping data of key populations collected during 1990-2015. Key populations assessed were heterosexual people (HET), men who have sex with men (MSM), people who inject drugs (PWID), vertical transmissions (VERT), commercial sex workers (CSW), and transfusion-associated infections (BLOOD). Logistic regression was used to determine associations of key populations with HIV-1 recombinants. Subgroup analyses were performed for circulating recombinant forms (CRFs), unique recombinant forms (URFs), regions, and time periods.

Results

Eight hundred and eighty five datasets including 77,284 participants from 83 countries were included. Globally, PWID were associated with the greatest odds of recombinants and CRFs (OR 2.6 [95% CI 2.46–2.74] and 2.99 [2.83–3.16]), compared to HET. CSW were associated with increased odds of recombinants and URFs (1.59 [1.44–1.75] and 3.61 [3.15–4.13]). VERT and BLOOD were associated with decreased odds of recombinants (0.58 [0.54–0.63] and 0.43 [0.33–0.56]). MSM were associated with increased odds of recombinants in 2010–2015 (1.43 [1.35–1.51]). Subgroup analyses supported our main findings.

Discussion

As PWID, CSW, and MSM are associated with HIV-1 recombinants, increased preventative measures and HIV-1 molecular surveillance are crucial within these key populations.

Systematic review registration

PROSPERO [CRD42017067164].
Realising distributed leadership through measurement for change
Authors: Watkins Jonathan , Muhamedjonova Nazira , Holding Penny A.
Published: August 2023

Through a systematic reflection on the journey that transformed traditional state-run baby homes in Tajikistan from closed institutions into community-oriented Family and Child Support Centres (FCSC) we reveal key moments of change. This review describes how community consultation with local participants in a development project shifted responsibility and accountability from international to local ownership and how distributed leadership contributes to the decolonisation of social services. Based on these interviews we ask, ‘How do the innovations of a social development project become a fixed part of normal local social, cultural and political life; and, how do we know when a new normal is self-sustaining at a local level?’ This analysis builds on a network-mapping tool previously described in this journal. Our interviews show that each participant has taken a non-linear journey, building on the networks previously described, under the influence of activities and discussions that emerged throughout the project. We consider how a monitoring, evaluation, and learning process should be responsive over time to these influences, rather than be set at the start of the project. Using the themes that emerge from participants’ journeys, we apply a ‘measurement for change’ (M4C) approach that integrates Monitoring, Evaluation and Learning (MEL) into decision-making. The journey framework applied represents a systematic application of the M4C approach that gives us insight into where local ownership is responsible for the sustainable management of the intervention, and where continued partnership will further strengthen impact and accountability. The exercise has provided evidence of progress towards decolonisation and of the centring of local priorities in MEL and implementation processes.
Change in grip strength, hang time, and knot tying speed after 24 hours of endurance rock climbing
Authors: Yu Elaine , Lowe Jacques , Millon Jasmin , Tran Kristi , Coffey Christanne
Published: August 2023

Background

Non-professional climbers are increasingly attempting long routes in a single day. Many suffer injury or rely on search and rescue teams when they become too fatigued to finish. Predicting fatigue is difficult, and existing studies have only studied climbers over durations less than an hour, while many outdoor multipitch climbs require more than an hour of climbing.

Objectives

To determine how strength, endurance, and dexterity reflect fatigue after 24 h of continuous climbing.

Methods

Volunteer competitors completed measurements of grip strength, static hang time to failure, and time to tie a figure-eight follow-through knot. Measurements were taken during the registration period before the competition and again within an hour after the competition ended. Measurements were compared using the pairedt-test. Subgroup analysis was applied to competitors by division. Linear regression was applied to determine the relationship between vertical feet climbed and the number of routes climbed during the competition on each metric.

Results

Thirty-six total climbers (average age 29.4 years old) completed pre- and post-competition measurements. After 24 h of climbing (n = 36), mean grip strength decreased by 14.3–15 lbs or 14.7%–15.1% (p < 0.001) and static hang time decreased by 54.2 s or 71.2% (p < 0.001). There was no significant change in time to tie a figure-eight-follow-through knot. Grip strength and hang time decreases were significant in climbers with outdoor redpoints of 5.10a and above. Hang time decreased by 5.4 s per 1,000 vertical feet climbed (p = 0.044).

Conclusion

Climbers can expect to experience a 14.7%–15.1% decrease in grip strength and 71.2% decrease in static hang time after 24 h of continuous climbing. These changes may make it difficult to climb consistently over a long objective, and climbers can use these measures at home to train for longer climbing routes. Future studies on shorter climbing intervals can help determine rates of decline in performance measures.
On the Range Equation for Hybrid-Electric Aircraft
Authors: Batra Aman , Raute Reiko , Camilleri Robert
Published: August 2023

This paper proposes a new range equation for hybrid-electric aircraft. The paper revisits the theory of the range equation for a hybrid-electric aircraft with constant power split published earlier in the literature and proposes a new efficiency-based definition of the degree of hybridization (φ), one which includes the efficiencies of the electric or fuel-powered drivetrain. The paper shows that the efficiencies of the respective drivetrains play a significant role in the range estimation of the hybrid-electric aircraft. The paper makes use of a case study to show the relationship between battery energy density, powertrain efficiency and modification in the definition of the degree of hybridization φ with aircraft range. We show that for every aircraft design, there is a battery energy density threshold, for which the aircraft range becomes independent of the degree of hybridization. Below this threshold, the range decreases with an increase in the degree of hybridization. Conversely, beyond this threshold, the aircraft range increases with the degree of hybridization. Our study finds that the new definition of φ has shifted this threshold significantly upwards compared to earlier publications in the literature. This makes the design of an aircraft with a high degree of hybridization less optimistic.
Study of Pose Estimation Based on Spatio-Temporal Characteristics of Cow Skeleton
Authors: Wei Yongfeng , Zhang Hanmeng , Gong Caili , Wang Dong , Ye Ming , Jia Yupu
Published: August 2023

The pose of cows reflects their body condition, and the information contained in the skeleton can provide data support for lameness, estrus, milk yield, and contraction behavior detection. This paper presents an algorithm for automatically detecting the condition of cows in a real farm environment based on skeleton spatio-temporal features. The cow skeleton is obtained by matching Partial Confidence Maps (PCMs) and Partial Affinity Fields (PAFs). The effectiveness of skeleton extraction was validated by testing 780 images for three different poses (standing, walking, and lying). The results indicate that the Average Precision of Keypoints (APK) for the pelvis is highest in the standing and lying poses, achieving 89.52% and 90.13%, respectively. For walking, the highest APK for the legs was 88.52%, while the back APK was the lowest across all poses. To estimate the pose, a Multi-Scale Temporal Convolutional Network (MS-TCN) was constructed, and comparative experiments were conducted to compare different attention mechanisms and activation functions. Among the tested models, the CMS-TCN with Coord Attention and Gaussian Error Linear Unit (GELU) activation functions achieved precision, recall, and F1 scores of 94.71%, 86.99%, and 90.69%, respectively. This method demonstrates a relatively high detection rate, making it a valuable reference for animal pose estimation in precision livestock farming.
Pruning and Fruit Thinning of Psidium guajava cv. Paluma under a Seasonal Tropical Climate
Authors: Santos Adaniel Sousa dos , Dalzot Jonathan Candido Thomaz , Pereira Gustavo Alves , Cunha Jenilton Gomes da , Evangelista Thamyres Yara Lima , Fonseca Wéverson Lima , Almeida Murilo de Sousa , Lacerda Julian Junior de Jesus , Filho Júlio Ferreira de Souza , Zuffo Alan Mario , Mezzomo Ricardo , Aguilera Jorge González , Morales-Aranibar Luis , Okla Mohammad K. , Saleh Ibrahim A. , AbdElgawad Hamada
Published: August 2023

Maintaining the plant architecture of Psidium guajava L. (guava tree) is essential for enhancing capture and distribution in the plant, directly affecting the fruit quality. The lifespan of the harvest period can be extended by proper pruning. Both timeliness and proper pruning play crucial roles in achieving high-quality fruit production and in maintaining a consistent fruit size while stimulating ascorbic acid levels, sugar content, total soluble solids (TSS), and titratable acidity. From this perspective, this study aimed to characterize the influence of different intensities of fruit pruning and thinning on guava trees grown under a seasonal tropical climate in two growing seasons in Currais, Piauí, Brazil. The experiment was set up in a randomized block design with a 3 × 3 × 2 factorial arrangement corresponding to short, medium, and long pruning intensities and 0%, 10%, and 20% thinning intensities during the two growth seasons, respectively. An analysis was performed to discriminate the treatment groups according to the physicochemical variables of the guava tree cv. Paluma and canonical discriminant analysis. There was significant variation in the SS, titratable acidity, ascorbic acid, and pH contents. Cluster analysis of all treatments allowed division into five different groups for the two pruning times. Canonical discriminant analysis showed that the first two canonical variables explained 91% of the total variance. The fruits of the second harvest exhibited a lower level of acidity, higher levels of soluble solids, and higher levels of ascorbic acid contents. In addition, these fruits also obtained better nutrient contents. Short pruning with up to 20% thinning, medium pruning with up to 10%, and long pruning without thinning favored better levels of macronutrients and micronutrients and, consequently, better fruit quality. Medium or long pruning with up to 20% thinning resulted in higher average fruit weights and nutrient contents (especially of Fe and Cu), lower acidity, and higher ascorbic acid contents. Thus, in general, the importance of production pruning in guava plants is evidenced and thinning of 20% is recommended to improve the fruit quality.
Explainable Image Classification: The Journey So Far and the Road Ahead
Authors: Kamakshi Vidhya , Krishnan Narayanan C.
Published: August 2023

Explainable Artificial Intelligence (XAI) has emerged as a crucial research area to address the interpretability challenges posed by complex machine learning models. In this survey paper, we provide a comprehensive analysis of existing approaches in the field of XAI, focusing on the tradeoff between model accuracy and interpretability. Motivated by the need to address this tradeoff, we conduct an extensive review of the literature, presenting a multi-view taxonomy that offers a new perspective on XAI methodologies. We analyze various sub-categories of XAI methods, considering their strengths, weaknesses, and practical challenges. Moreover, we explore causal relationships in model explanations and discuss approaches dedicated to explaining cross-domain classifiers. The latter is particularly important in scenarios where training and test data are sampled from different distributions. Drawing insights from our analysis, we propose future research directions, including exploring explainable allied learning paradigms, developing evaluation metrics for both traditionally trained and allied learning-based classifiers, and applying neural architectural search techniques to minimize the accuracy–interpretability tradeoff. This survey paper provides a comprehensive overview of the state-of-the-art in XAI, serving as a valuable resource for researchers and practitioners interested in understanding and advancing the field.
A Retrospective Study on Canine and Feline Mortality during Anaesthesia at a University Clinic in Greece
Authors: Varkoulis Konstantinos , Savvas Ioannis , Anagnostou Tilemachos , Kazakos George , Pavlidou Kiriaki
Published: August 2023

This retrospective cohort study investigated the mortality rate during anaesthesia and possible contributing factors in canine and feline population in an academic institution in Greece. Data on 1187 dogs and 250 cats which underwent general anaesthesia from 1 January 2018 to 31 December 2019 at the Veterinary Faculty of the Aristotle University of Thessaloniki were analysed regarding cardiac arrest and mortality. In dogs, the rate of cardiac arrest was 1.1% and the rate of death was 0.6%. In cats, these rates were 2.8% and 0.8%, respectively. The mortality rate in healthy/mild disease (ASA I-II) dogs was 0.1% and in cats was 0.5%. Sick (ASA III-V) dogs exhibited a death rate of 2.6%, while sick cats had a rate of 2.2%. In dogs, ASA status had a positive association with cardiac arrest and mortality, with sick dogs being 23 times more likely to suffer cardiac arrest and 24.5 times more likely to die than healthy/mild disease ones. Other factors associated with cardiac arrest and mortality were the anaesthetic protocol and the use of inotropes. In cats, premedication and inotropic support were related to cardiac arrest and death. Feline anaesthesia involves higher risk, and requires greater vigilance in peri-anaesthetic management than dogs.
In Vitro Activity of Two Novel Antimicrobial Compounds on MDR-Resistant Clinical Isolates
Authors: Rima Mariam , Pfennigwerth Niels , Cremanns Martina , Cirnski Katarina , Oueslati Saoussen , Gatermann Sören G. , d’Amélio Nicola , Herrmann Jennifer , Müller Rolf , Naas Thierry
Published: August 2023

The development of novel antibiotics is mandatory to curb the growing antibiotic resistance problem resulting in difficult-to-treat bacterial infections. Here, we have determined the spectrum of activity of cystobactamids and chelocardins, two novel and promising classes of molecules with different modes of action. A panel of 297 clinically relevant Gram-negative and Gram-positive isolates with different antibiotic susceptibility profiles, going from wild type to multi- or even extremely drug resistant (MDR, XDR) and including carbapenem-resistant isolates, were tested using broth microdilution assays to determine the minimal inhibitory concentrations (MICs), MIC50s and MIC90s of two cystobactamids derivatives (CN-861-2 and CN-DM-861) and two chelocardin derivatives (CHD and CDCHD). Cystobactamids revealed potent activities on the majority of tested Enterobacterales (MIC50s ranging from 0.25 to 4 µg/mL), except for Klebsiella pneumoniae isolates (MIC50s is 128 µg/mL). Pseudomonas aeruginosa and Acinetobacter baumannii showed slightly higher MIC50s (4 µg/mL and 8 µg/mL, respectively) for cystobactamids. Chelocardins inhibited the growth of Enterobacterales and Stenotrophomas maltophilia at low to moderate MICs (0.25–16 µg/mL) and the chemically modified CDCHD was active at lower MICs. A. baumannii and P. aeruginosa were less susceptible to these molecules with MICs ranging from 0.5 to 32 µg/mL. These molecules show also interesting in vitro efficacies on clinically relevant Gram-positive bacteria with MICs of 0.125–8 µg/mL for cystobactamids and 0.5–8 µg/mL for chelocardins. Taken together, the cystobactamid CN-DM-861 and chelocardin CDCHD showed interesting antibiotic activities on MDR or XDR bacteria, without cross-resistance to clinically relevant antibiotics such as carbapenems, fluoroquinolones, and colistin.
Antimicrobial-Resistant Bacteria from Free-Living Green Turtles (Chelonia mydas)
Authors: Short Fernanda S. , Lôbo-Hajdu Gisele , Guimarães Suzana M. , Laport Marinella S. , Silva Rosane
Published: August 2023

Bioindicator species are used to assess the damage and magnitude of possible impacts of anthropic origin on the environment, such as the reckless consumption of antimicrobials. Chelonia mydas has several characteristics that make it a suitable bioindicator of marine pollution and of the presence of pathogens that cause diseases in humans. This study aimed to investigate the green sea turtle as a reservoir of resistant bacteria, mainly because C. mydas is the most frequent sea turtle species in Brazilian coastal regions and, consequently, under the intense impact of anthropic factors. Free-living green sea turtles ranging from 42.8 to 92 cm (average = 60.7 cm) were captured from Itaipú Beach, Brazil. Cloaca samples (characterizing the gastrointestinal tract) and neck samples (representing the transient microbiota) were collected. Bacterial species were identified, and their was resistance associated with the antimicrobials cephalothin, ciprofloxacin, gentamicin, tetracycline, and vancomycin. Citrobacter braaki, Klebsiella oxytoca, K. variicola and Proteus mirabilis were found resistant to cephalothin and Morganella morganii and Enterococcus faecalis tetracycline-resistant isolates in cloaca samples. In neck samples, species resistant to tetracycline were Salmonella sp., Serratia marcescens, S. ureylitica and Proteus mirabilis. This data reinforces that the green turtle is a bioindicator of antimicrobial resistance (AMR).
CCN2 Activates RIPK3, NLRP3 Inflammasome, and NRF2/Oxidative Pathways Linked to Kidney Inflammation
Authors: Rayego-Mateos Sandra , Marquez-Exposito Laura , Basantes Pamela , Tejedor-Santamaria Lucia , Sanz Ana B. , Nguyen Tri Q. , Goldschmeding Roel , Ortiz Alberto , Ruiz-Ortega Marta
Published: August 2023

Inflammation is a key characteristic of both acute and chronic kidney diseases. Preclinical data suggest the involvement of the NLRP3/Inflammasome, receptor-interacting protein kinase-3 (RIPK3), and NRF2/oxidative pathways in the regulation of kidney inflammation. Cellular communication network factor 2 (CCN2, also called CTGF in the past) is an established fibrotic biomarker and a well-known mediator of kidney damage. CCN2 was shown to be involved in kidney damage through the regulation of proinflammatory and profibrotic responses. However, to date, the potential role of the NLRP3/RIPK3/NRF2 pathways in CCN2 actions has not been evaluated. In experimental acute kidney injury induced with folic acid in mice, CCN2 deficiency diminished renal inflammatory cell infiltration (monocytes/macrophages and T lymphocytes) as well as the upregulation of proinflammatory genes and the activation of NLRP3/Inflammasome-related components and specific cytokine products, such as IL-1β. Moreover, the NRF2/oxidative pathway was deregulated. Systemic administration of CCN2 to C57BL/6 mice induced kidney immune cell infiltration and activated the NLRP3 pathway. RIPK3 deficiency diminished the CCN2-induced renal upregulation of proinflammatory mediators and prevented NLRP3 modulation. These data suggest that CCN2 plays a fundamental role in sterile inflammation and acute kidney injury by modulating the RIKP3/NLRP3/NRF2 inflammatory pathways.
Magnetoelectric Composites: Engineering for Tunable Filters and Energy Harvesting Applications
Authors: Kozielski Lucjan , Bochenek Dariusz , Clemens Frank , Sebastian Tutu
Published: August 2023

Multiferroic ceramic composites have been engineered to incorporate multiple desired physical properties within a single ceramic component. The objective of this study was to create such composites through pressure less sintering ferroelectric-doped PZT and nickel–zinc ferrite at a temperature of 1250 °C. The growth of ferrite grains was found to be influenced by the concentration of the ferroelectric PZT phase. Consequently, an increase in the ferrite content decreased the average particle size of nickel–zinc ferrite by a factor of 1.8. After impedance spectroscopy, the multiferroic ceramic composites can be categorized into two groups: those with low ferrite content (<20%) and those with a high ferrite content (>20%). Composites with a high ferrite content are suitable for dual-band filters or shield applications. The impedance spectroscopy analysis revealed that the resonance frequency can be shifted to higher frequency ranges. Therefore, it was demonstrated that modifying the composition of the multiferroic composite allows for tailoring the impedance behavior to shield living and working spaces against such radiation to meet the demands of the 21st century.