bluuebunny/crossref_metadata_2025 huggingface dataset:
A conceptual framework for Emergency department design in a pandemic
Authors: Nadarajan Gayathri Devi , Omar Eunizar , Abella Benjamin S. , Hoe Pei Shan , Do Shin Sang , Ma Matthew Huei-Ming , Ong Marcus Eng Hock
Published: December 2020

Background

The current COVID-19 pandemic is highlighting gaps around the world in the design and workflow of Emergency Departments (ED). These gaps have an impact on both patient care and staff safety and represent a risk to public health. There is a need for a conceptual framework to guide ED design and workflow to address these challenges. Such a framework is important as the ED environment will always remain vulnerable to infectious diseases outbreaks in the future.

Aims

This paper aims to address issues and principles around ED design and workflow amidst the COVID-19 pandemic. We propose a conceptual framework and checklist for EDs to be prepared for future outbreaks as well.

Methods

A scoping literature review was conducted, of the experiences of EDs in managing outbreaks such as SARS, H1N1 and COVID-19. The combined experiences of the authors and the experiences from the literature were grouped under common themes to develop the conceptual framework.

Results

Four key principles were derived- (1) situational awareness, surveillance and perimeter defence, (2) ED staff protection, (3) surge capacity management and (4) ED recovery. The findings were integrated in a proposed conceptual framework to guide ED design in response to an infectious disease outbreak. There are various elements which need to be considered at ED input, throughput and output. These elements can be categorised into (1)system(workflow, protocols and communication), (2)staff(human resources), (3)space(infrastructure), and (4)supply(logistics) and are placed in a checklist for pragmatic use.

Conclusion

The ED needs to be in a constant state of preparedness. A framework can be useful to guide ED design and workflow to achieve this. As all ED systems are different with varying capabilities, our framework may help EDs across the world prepare for infectious disease outbreaks.
FAN-C: a feature-rich framework for the analysis and visualisation of chromosome conformation capture data
Authors: Kruse Kai , Hug Clemens B. , Vaquerizas Juan M.
Published: December 2020

Chromosome conformation capture data, particularly from high-throughput approaches such as Hi-C, are typically very complex to analyse. Existing analysis tools are often single-purpose, or limited in compatibility to a small number of data formats, frequently making Hi-C analyses tedious and time-consuming. Here, we present FAN-C, an easy-to-use command-line tool and powerful Python API with a broad feature set covering matrix generation, analysis, and visualisation for C-like data (https://github.com/vaquerizaslab/fanc). Due to its compatibility with the most prevalent Hi-C storage formats, FAN-C can be used in combination with a large number of existing analysis tools, thus greatly simplifying Hi-C matrix analysis.
Evaluation of students' attitude and emotions towards the sudden closure of schools during the COVID-19 pandemic: a cross-sectional study
Authors: Mirahmadizadeh Alireza , Ranjbar Keivan , Shahriarirad Reza , Erfani Amirhossein , Ghaem Haleh , Jafari Khoubyar , Rahimi Tayebeh
Published: December 2020

Background

Rapid increases in the number of COVID-19 cases have led to the closure of academic institutions including elementary and high schools. The absence from the educational environment can affect the students’ emotions towards education and school attendance. In this study, we aimed to evaluate the students' attitude and emotions towards the sudden closure of schools during the COVID-19 pandemic.

Methods

By utilizing a modified version of the Achievement Emotions Questionnaire, a web-based cross-sectional study was conducted to evaluate the students' positive and negative emotions towards schools during the early phases of the COVID-19 pandemic.

Results

Questionnaires were filled by 20,697 participants with an average age of 13.76, and 70.3% of them were females. Also, 83.3% were from public schools and 80.6% from urban areas. Based on the questionnaire, for positive attitude a median of 31 (IQR 26–35) and for negative attitude a median of 25 (IQR 19–32) were obtained.

Conclusion

Our findings demonstrated a satisfactory level of students' emotions regarding schools and education during the closure of schools and institutions. This indicates that despite the imposed situation, students showed enthusiasm towards learning and schools.
Associations of time-restricted eating with health-related quality of life and sleep in adults: a secondary analysis of two pre-post pilot studies
Authors: Kesztyüs Dorothea , Fuchs Monika , Cermak Petra , Kesztyüs Tibor
Published: December 2020

Background

Therapeutic fasting may improve health-related quality of life (HRQoL) and sleep but is not applicable for everyone. Time-restricted eating (TRE) offers a low threshold alternative but research on associations with HRQoL and sleep is rare.

Methods

We conducted a secondary analysis of two pilot studies in a pre-post design, which examined TRE in healthy employees at the Ulm University and in abdominal obese patients in a general practitioners office. Participants reported their HRQoL (EQ-5D visual analogue scale) before and after 3 months of restricting their daily eating to 8–9 h. They kept a diary to protocol timing of first and last meal, sleep quality (analogue scale) and duration. Pearson’s correlation coefficient was applied to test bivariate correlations between continuous variables and linear regression analyses were conducted to identify associated factors with the pre-post differences in HRQoL and the differences in sleep quality.

Results

Ninety-nine participants (aged aged 48.9 ± 1.1, 83.8% female) reached the fasting target of 15–16 h on average on 77.2 ± 18.7% of all recorded days. HRQoL increased by 7.8 ± 12.6 and sleep quality by 9.6 ± 13.9 points, but sleep duration was not extended. Regression analysis revealed mean fasting duration and baseline sleep quality as significant factors associated with changes in HRQoL. Improvements in sleep quality correlated with baseline sleep quality and HRQoL at follow-up but not with fasting. Changes in anthropometry did not correlate with the HRQoL or sleep quality.

Conclusions

TRE correlates with increased HRQoL and sleep quality independent from weight loss. TRE is easily applicable with or without medical supervision. The potential effects of TRE on health and sleep should be further investigated in larger randomized trials.

Trial registration

German Register for Clinical Trials (DRKS), DRKS-ID: DRKS00015057. Registered 4 July 2018.
Transference-focused psychotherapy as an aid to learning psychodynamic psychotherapy: qualitative analysis of UK psychiatry trainees’ views
Authors: Kanter Bax Orestis , Nerantzis Georgios , Lee Tennyson
Published: December 2020

Aims & method

Learning psychotherapy can be difficult and stressful. We explore core trainees’ (n = 5) views on undertaking a psychodynamic psychotherapy training case using transference-focused psychotherapy (TFP), in an East London NHS Foundation Trust supervision group. We used framework analysis of focus group interviews to examine trainees’ concerns, their views about this experience and its impact on general psychiatric practice.

Results

Trainees described various concerns on starting: providing an effective intervention, insufficient experience and training-related pressures. However, they found that TFP addressed some of them and was helpful for learning psychodynamic psychotherapy. Difficulties around the countertransference remained at end-point. Trainees suggested that introductory teaching and learning through observation might be worthwhile.

Clinical implications

Trainees’ experience suggests that an evidence-based operationalised approach such as TFP can be integrated into the core psychiatry curriculum as a psychodynamic psychotherapy learning method. Trainees report benefits extending to other areas of their practice.
Association Between Preoperative Statin Use and Respiratory Complications After Noncardiac Surgery: A Retrospective Cohort Analysis
Authors: Komatsu Ryu , Yilmaz Huseyin Oguz , Makarova Natalya , Turan Alparslan , Sessler Daniel I. , Rajan Shobana , Argalious Maged
Published: December 2020

BACKGROUND:

Statins possess pleiotropic effects, which potentially benefit noncardiovascular conditions. Previous work suggests that statins reduce inflammation and prevent acute respiratory distress syndrome and infections. However, there is a paucity of data regarding potential benefits of statins on respiratory and infectious complications, particularly after noncardiac surgery. We therefore evaluated respiratory and other complications in noncardiac surgery patients taking or not taking statins preoperatively.

METHODS:

We obtained data from the Cleveland Clinic Perioperative Health Documentation System and evaluated medical records of 92,139 inpatients who had noncardiac surgery. Among these, 31,719 patients took statins preoperatively. Statin patients were compared to nonstatin patients on incidence of intraoperative use of albuterol and postoperative respiratory complications for primary analysis. Infectious complications, cardiovascular complications, in-hospital mortality, and duration of hospitalization were compared for secondary analyses, using inverse probability of treatment weighting to control for potential confounding.

RESULTS:

Statin use was associated with lower odds of intraoperative albuterol treatment (odds ratio [OR] = 0.89; 97.5% confidence interval [CI], 0.82–0.97; P = .001; number needed to treat [NNT] = 216). Postoperative respiratory complications were also less common (OR = 0.82; 98.75% CI, 0.78–0.87; P < .001). Secondarily, statin use was associated with lower odds of infections, cardiovascular complications, in-hospital mortality, and shorter duration of hospitalization. The interaction between statin use and sex was significant (with significance criteria P < .10) for all primary and secondary outcomes except intraoperative use of albuterol.

CONCLUSIONS:

Preoperative statin use in noncardiac surgical patients was associated with slightly reduced odds of postoperative respiratory, infectious, and cardiovascular complications. However, the NNTs were high. Thus, despite the fact that statins appeared to be associated with lower odds of various complications, especially cardiovascular complications, our results do not support using statins specifically to reduce noncardiovascular complications after noncardiac surgery.
Emerging Role of Long Noncoding RNAs in Perioperative Neurocognitive Disorders and Anesthetic-Induced Developmental Neurotoxicity
Authors: Pant Tarun , DiStefano Johanna K. , Logan Sara , Bosnjak Zeljko J.
Published: December 2020

Preclinical investigations in animal models have consistently demonstrated neurobiological changes and life-long cognitive deficits following exposure to widely used anesthetics early in life. However, the mechanisms by which these exposures affect brain function remain poorly understood, therefore, limiting the efficacy of current diagnostic and therapeutic options in human studies. The human brain exhibits an abundant expression of long noncoding RNAs (lncRNAs). These biologically active transcripts play critical roles in a diverse array of functions, including epigenetic regulation. Changes in lncRNA expression have been linked with brain development, normal CNS processes, brain injuries, and the development of neurodegenerative diseases, and many lncRNAs are known to have brain-specific expression. Aberrant lncRNA expression has also been implicated in areas of growing importance in anesthesia-related research, including anesthetic-induced developmental neurotoxicity (AIDN), a condition defined by neurological changes occurring in patients repeatedly exposed to anesthesia, and the related condition of perioperative neurocognitive disorder (PND). In this review, we detail recent advances in PND and AIDN research and summarize the evidence supporting roles for lncRNAs in the brain under both normal and pathologic conditions. We also discuss lncRNAs that have been linked with PND and AIDN, and conclude with a discussion of the clinical potential for lncRNAs to serve as diagnostic and therapeutic targets for the prevention of these neurocognitive disorders and the challenges facing the identification and characterization of associated lncRNAs.
Racial Disparities in Failure to Rescue Following Unplanned Reoperation in Pediatric Surgery
Authors: Willer Brittany L. , Mpody Christian , Tobias Joseph D. , Nafiu Olubukola O.
Published: December 2020

BACKGROUND:

Failure to rescue (FTR) and unplanned reoperation following an index surgical procedure are key indicators of the quality of surgical care. Given that differences in unplanned reoperation and FTR rates among racial groups may contribute to persistent disparities in postsurgical outcomes, we sought to determine whether racial differences exist in the risk of FTR among children who required unplanned reoperation following inpatient surgical procedures.

METHODS:

We used the National Surgical Quality Improvement database (2012–2017) to assemble a cohort of children (<18 years), who underwent inpatient surgery and subsequently returned to the operating room within 30 days of the index surgery. We used logistic regression models to estimate the odds ratio (OR) and 95% confidence interval (CI) of FTR, comparing African American (AA) to White children. We estimated the risk-adjusted odds ratio (aOR) for FTR by controlling the analyses for demographic characteristics, surgical profile, and preoperative comorbidities. We further evaluated the racial differences in FTR by stratifying the analyses by the timing of unplanned reoperation.

RESULTS:

Of 276,917 children who underwent various inpatient surgical procedures, 10,425 (3.8%) required an unplanned reoperation, of whom 2016 (19.3%) were AA and 8409 (80.7%) were White. Being AA relative to being White was associated with a 2-fold increase in the odds of FTR (aOR: 2.03; 95% CI, 1.5–2.74; P < .001). Among children requiring early unplanned reoperation, AAs were 2.38 times more likely to die compared to their White peers (8.9% vs 3.4%; aOR: 2.38; 95% CI, 1.54–3.66; P < .001). In children with intermediate timing of return to the operating room, the risk of FTR was 80% greater for AA children compared to their White peers (2.2% vs 1.1%; aOR: 1.80; 95% CI, 1.07–3.02; P = .026). Typically, AA children die within 5 days (interquartile range [IQR]: 1–16) of reoperation while their White counterparts die within 9 days following reoperation (IQR: 2–26).

CONCLUSIONS:

Among children requiring unplanned reoperation, AA patients were more likely to die than their White peers. This racial difference in FTR rate was most noticeable among children requiring early unplanned reoperation. Time to mortality following unplanned reoperation was shorter for AA than for White children. Race appears to be an important determinant of FTR following unplanned reoperation in children and it should be considered when designing interventions to optimize unplanned reoperation outcomes.
Antihomosexuality and Nationalist Critique in Late Colonial Jamaica
Authors: Chin Matthew
Published: December 2020

This essay examines discourses of homosexuality in late colonial Jamaica through an analysis of the 1951 Police Enquiry, which leveraged accusations of homosexuality among Jamaica’s foreign police officers as a key component of its investigative work. With information from Jamaican state records, news media, literature, and social science studies, the essay argues that the inquiry mobilized divergent discourses of homosexuality across the Atlantic to enact an anticolonial nationalist form of sexual regulation. The inquiry drew not only from Jamaican figurations of homosexuality as the preserve of wealthy white foreign men but also from the Wolfenden Committee proceedings that led to the decriminalization of homosexuality in England and from the “Lavender Scare” that purged homosexuals from federal government employment in the United States. Despite its failing to reform Jamaica’s police force, the inquiry nevertheless foregrounds how sexual regulation operates through the interconnected workings of race, class, gender, and nation.
Diagnostics of the stochastic ionospheric channel in the decameter band of radio waves
Authors: Afanasiev Nikolay , Chudaev Stanislav
Published: December 2020

We propose a method for direct diagnostics of a stochastic ionospheric radio channel. This method can recalculate probe signal characteristics into transmitted signal characteristics. We derive analytical equations of second-order statistical moments for trajectory characteristics of the main and probe signals propagating in a three-dimensional randomly inhomogeneous ionosphere. We take into account boundary conditions at signal transmission and reception points. As a model of random irregularities of permittivity of the ionosphere, we utilize the concept of a changing space-time correlation ellipsoid, which is self-consistent with spatial changes in the average ionosphere. Time fluctuations of random irregularities are taken into account by the hypothesis of frozen transfer. We use analytical relationships to calculate the expected statistical characteristics of decameter signals along oblique probing paths of the ionosphere. An operational numerical algorithmization of the formulas derived is proposed. We report results of numerical experiments to determine the expected phase variances, group delay, and Doppler frequency shift of the main signal on a given single-hop path, based on measurements of these characteristics of a probe signal on a secondary path. We demonstrate the efficiency of the proposed method for diagnosing statistical trajectory characteristics of a decameter signal along single-hop paths under conditions when ground points of transmission and reception of the main and probe signals are outside the vicinity of focusing points of the wave field.
Earthquakes and geomagnetic disturbances
Authors: Guglielmi Anatol , Klain Boris , Kurazhkovskaya Nadezhda
Published: December 2020

The article addresses the problem of the connection of earthquakes with geomagnetic phenomena. We have carried out an experimental study using a method based, firstly, on the separation of periods of geomagnetic activity into extremely quiet and disturbed, and, secondly, on the description of seismic activity with an index called the global daily magnitude (GDM). By analyzing the NEIC earthquake catalog of the US Geological Survey over a 20-year period from 1980 to 1999, we have shown that the planetary activity of earthquakes under extremely quiet geomagnetic conditions is noticeably higher than under disturbed conditions. The detected tendency for seismic activity to increase in extremely quiet periods of geomagnetic activity has indirectly been confirmed by the analysis of 35 earthquakes with magnitude 8 and higher, which occurred on Earth from 1980 to 2019. We have found that in extremely quiet geomagnetic conditions, the probability of the occurrence of strong earthquakes is noticeably higher. The result qualitatively confirms the assumption of a change in the regime of seismic activity due to the influence of alternating magnetic fields on the ductility of rocks.
Active foot placement control ensures stable gait: Effect of constraints on foot placement and ankle moments
Authors: van Leeuwen A. M. , van Dieën J. H. , Daffertshofer A. , Bruijn S. M.
Published: December 2020

Step-by-step foot placement control, relative to the center of mass (CoM) kinematic state, is generally considered a dominant mechanism for maintenance of gait stability. By adequate (mediolateral) positioning of the center of pressure with respect to the CoM, the ground reaction force generates a moment that prevents falling. In healthy individuals, foot placement is complemented mainly by ankle moment control ensuring stability. To evaluate possible compensatory relationships between step-by-step foot placement and complementary ankle moments, we investigated the degree of (active) foot placement control during steady-state walking, and under either foot placement-, or ankle moment constraints. Thirty healthy participants walked on a treadmill, while full-body kinematics, ground reaction forces and EMG activities were recorded. As a replication of earlier findings, we first showed step-by-step foot placement is associated with preceding CoM state and hip ab-/adductor activity during steady-state walking. Tight control of foot placement appears to be important at normal walking speed because there was a limited change in the degree of foot placement control despite the presence of a foot placement constraint. At slow speed, the degree of foot placement control decreased substantially, suggesting that tight control of foot placement is less essential when walking slowly. Step-by-step foot placement control was not tightened to compensate for constrained ankle moments. Instead compensation was achieved through increases in step width and stride frequency.
Development and initial validation of a simple tool to screen for partner support or opposition to HIV prevention product use
Authors: Tolley Elizabeth E. , Zissette Seth , Martinez Andres , Palanee-Phillips Thesla , Mathebula Florence , Tenza Siyanda , Hartmann Miriam , Montgomery Elizabeth T.
Published: December 2020

In HIV prevention trials, male partners have influenced women’s ability to adhere to investigational products, including antiretroviral (ARV) containing vaginal rings. Validated scales can be useful tools to systematically measure complex constructs, such as those related to male partner engagement. Although multiple scales exist to assess physical, psychological and sexual violence within intimate relationships, fewer scales focus on supportive behaviors within these relationships. Our intervention involved development of a Healthy Relationship Assessment Tool (HEART) that assessed both positive and negative aspects of male partner involvement in women’s HIV prevention. We identified and refined 127 potential items, representing intimate partner violence, agency and social support. A structured survey, including potential items and other sociodemographic and behavioral variables was administered to former microbicide trial and non-trial participants. We conducted an exploratory factor analysis (EFA) to identify a reduced set of constructs and items to screen women who might experience social harms or benefits from vaginal ring use. We examined associations between constructs and with other survey variables to assess content and construct validity. In a subset of 10 women who participated in the survey and qualitative interviews, we used qualitative data to predict survey scores. We retained five constructs with theoretical relevance and good-to-strong reliability for the tool, including: Traditional Values; Partner Support; Partner Abuse & Control; Partner Resistance to HIV Prevention; and HIV Prevention Readiness. Predicted associations between HEART constructs, and correspondence between participants’ qualitative data and HEART scores were generally correct, while those between constructs and other sociodemographic variables were more mixed. Initial validation of the HEART tool was promising. The tool will be used during the CHARISMA pilot study at the Johannesburg MTN 025/HOPE site and validated as part of a randomized controlled trial of CHARISMA within a PrEP demonstration project. Beyond clinical trial settings, HEART could assist PrEP or antiretroviral treatment (ART) providers with an easy-to-administer tool to identify risk and tailor risk reduction, empowerment and adherence counseling for microbicides, PrEP or ART related services.
Estimation of finite population distribution function with dual use of auxiliary information under non-response
Authors: Hussain Sardar , Ahmad Sohaib , Akhtar Sohail , Javed Amara , Yasmeen Uzma
Published: December 2020

In this paper, we propose two new families of estimators for estimating the finite population distribution function in the presence of non-response under simple random sampling. The proposed estimators require information on the sample distribution functions of the study and auxiliary variables, and additional information on either sample mean or ranks of the auxiliary variable. We considered two situations of non-response (i) non-response on both study and auxiliary variables, (ii) non-response occurs only on the study variable. The performance of the proposed estimators are compared with the existing estimators available in the literature, both theoretically and numerically. It is also observed that proposed estimators are more precise than the adapted distribution function estimators in terms of the percentage relative efficiency.
A spatial-temporal description of the SARS-CoV-2 infections in Indonesia during the first six months of outbreak
Authors: Aisyah Dewi Nur , Mayadewi Chyntia Aryanti , Diva Haniena , Kozlakidis Zisis , Siswanto  , Adisasmito Wiku
Published: December 2020

Background

Since the first cases reported in Wuhan, China, in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide. In Indonesia, the first case was reported in early March 2020, and the numbers of confirmed infections have been increasing until now. Efforts to contain the virus globally and in Indonesia are ongoing. This is the very first manuscript using a spatial-temporal model to describe the SARS-CoV-2 transmission in Indonesia, as well as providing a patient profile for all confirmed COVID-19 cases.

Method

Data was collected from the official website of the Indonesia National Task Force for the Acceleration of COVID-19, from the period of 02 March 2020–02 August 2020. The data from RT-PCR confirmed, SARS-CoV-2 positive patients was categorized according to demographics, symptoms and comorbidities based on case categorization (confirmed, recovered, dead). The data collected provides granular and thorough information on time and geographical location for all 34 Provinces across Indonesia.

Results

A cumulative total of 111,450 confirmed cases of were reported in Indonesia during the study period. Of those confirmed cases 67.79% (75,551/111,450) were shown as recovered and 4.83% (5,382/111,450) of them as died. Patients were mostly male (50.52%; 56,300/111,450) and adults aged 31 to 45 years old (29.73%; 33,132/111,450). Overall patient presentation symptoms of cough and fever, as well as chronic disease comorbidities were in line with previously published data from elsewhere in South-East Asia. The data reported here, shows that from the detection of the first confirmed case and within a short time period of 40 days, all the provinces of Indonesia were affected by COVID-19.

Conclusions

This study is the first to provide detailed characteristics of the confirmed SARS-CoV-2 patients in Indonesia, including their demographic profile and COVID-19 presentation history. It used a spatial-temporal analysis to present the epidemic spread from the very beginning of the outbreak throughout all provinces in the country. The increase of new confirmed cases has been consistent during this time period for all provinces, with some demonstrating a sharp increase, in part due to the surge in national diagnostic capacity. This information delivers a ready resource that can be used for prediction modelling, and is utilized continuously by the current Indonesian Task Force in order to advise on potential implementation or removal of public distancing measures, and on potential availability of healthcare capacity in their efforts to ultimately manage the outbreak.
Level of sex hormones and their association with acetylsalicylic acid intolerance and nasal polyposis
Authors: Espersen Julia , Weber Ursula , Römer-Franz Ariane , Lenarz Thomas , Stolle Stefan R. O. , Warnecke Athanasia
Published: December 2020

Background

Chronic rhinosinusitis may be associated with nasal polyposis. Recurrence of disease is often observed and may be due to an intolerance of acetylsalicylic acid. Sex hormones are known to modulate allergic reactions and inflammation. Whether they may be involved in the development and progression of nasal polyposis has not been investigated yet.

Aim

Examine the relationship between levels of sex hormones and nasal polyposis.

Methods

Hormonal levels (estradiol, testosterone and progesterone) in patients with nasal polyposis (n = 26) with or without acetylsalicylic acid-intolerance were determined and compared to hormonal levels in patients with septal deviation (n = 35). Cone-beam computed tomography scans were analysed by using scores as defined by Lund and Mackay and by Kennedy.

Results

Our results show a 5 times greater odds (p = 0.01) for developing nasal polyposis in the presence of lowered estradiol plasma levels than in the presence of normal / elevated levels. When analyzing females and males separately, a 6 times greater odds for females to develop nasal polyposis in the presence of lowered estradiol plasma levels was calculated (p = 0.02). Thus, females are more likely to develop nasal polyposis when they have lowered estradiol levels than males. In addition, female patients showed an increased risk for developing ASA intolerance (p = 0.01).

Conclusion

Variation of sex hormones may be involved in nasal polyposis. Further studies including more patients to validate the presented results are required.

Significance

Retrospective clinical investigation suggesting a correlation between varying sex hormones and nasal polyposis.
Complete genome reveals genetic repertoire and potential metabolic strategies involved in lignin degradation by environmental ligninolytic Klebsiella variicola P1CD1
Authors: dos Santos Melo-Nascimento Amanda Oliveira , Mota Moitinho Sant´Anna Brena , Gonçalves Carolyne Caetano , Santos Giovanna , Noronha Eliane , Parachin Nádia , de Abreu Roque Milton Ricardo , Bruce Thiago
Published: December 2020

Lignin is a recalcitrant macromolecule formed by three alcohols (monolignols) predominantly connected by β-aryl ether linkages and is one of the most abundant organic macromolecules in the biosphere. However, the role played by environmental bacteria in lignin degradation is still not entirely understood. In this study, we identified an environmental Klebsiella strain isolated from sediment collected from an altitudinal region in a unique Brazilian biome called Caatinga. This organism can also grow in the presence of kraft lignin as a sole source of carbon and aromatic compounds. We performed whole-genome sequencing and conducted an extensive genome-based metabolic reconstruction to reveal the potential mechanisms used by the bacterium Klebsiella variicola P1CD1 for lignin utilization as a carbon source. We identified 262 genes associated with lignin-modifying enzymes (LMEs) and lignin-degrading auxiliary enzymes (LDAs) required for lignin and aromatic compound degradation. The presence of one DyP (Dye-decolorizing Peroxidase) gene suggests the ability of P1CD1 strain to access phenolic and nonphenolic structures of lignin molecules, resulting in the production of catechol and protocatechuate (via vanillin or syringate) along the peripheral pathways of lignin degradation. K. variicola P1CD1 uses aldehyde-alcohol dehydrogenase to perform direct conversion of vanillin to protocatechol. The upper funneling pathways are linked to the central pathways of the protocatechuate/catechol catabolic branches via β-ketoadipate pathways, connecting the more abundant catabolized aromatic compounds with essential cellular functions, such as energy cellular and biomass production (i.e., via acetyl-CoA formation). The combination of phenotypic and genomic approaches revealed the potential dissimilatory and assimilatory ability of K. variicola P1CD1 to perform base-catalyzed lignin degradation, acting on high- and low-molecular-weight lignin fragments. These findings will be relevant for developing metabolic models to predict the ligninolytic mechanism used by environmental bacteria and shedding light on the flux of carbon in the soil.
Inter-floor noise classification using convolutional neural network
Authors: Shin Hye-kyung , Park Sang Hee , Kim Kyoung-woo
Published: December 2020

In apartment houses, noise between floors can disturb pleasant living environments and cause disputes between neighbors. As a means of resolving disputes caused by inter-floor noise, noises are recorded for 24 hours in a household to verify whether the inter-floor noise exceeded the legal standards. If the noise exceeds the legal standards, the recorded sound is listened to, and it is checked whether the noise comes from neighboring households. When done manually, this process requires time and is costly, and there is a problem of whether the listener’s judgments of the sound source are consistent. This study aims to classify inter-floor noise according to noise sources by using a convolutional neural network model. A total of 1,515 sound sources of data recorded for 24 h from three households were annotated, and 40 4s audio clips of six noise sources, including “Footsteps,” “Dragging furniture,” “Hammering,” “Instant impact (dropping a heavy item),” “Vacuum cleaner,” and “Public announcement system” were identified. Moreover, datasets of 16 classes using ESC50’s urban sound category audio were used to distinguish the inter-floor noise heard indoors from the external noise. Although DenseNet, ResNet, Inception, and EfficientNet are models that use images as their domains, they showed an accuracy of 91.43–95.27% when classifying the inter-floor noise dataset. Among the reviewed models, ResNet showed an accuracy of 95.27±2.30% as well as a highest performance level in the F1 score, precision, and recall metrics. Additionally, ResNet showed the shortest inference time. This paper concludes by suggesting that the present findings can be extended in future research for monitoring acoustic elements of indoor soundscape.
A genealogical approach to academic success
Authors: Wuestman Mignon , Frenken Koen , Wanzenböck Iris
Published: December 2020

We analyse academic success using a genealogical approach to the careers of over 95,000 scientists in mathematics and associated fields in physics and chemistry. We look at the effect of Ph.D. supervisors (one’s mentors) on the number of Ph.D. students that one supervises later on (one’s mentees) as a measure of academic success. Supervisors generally provide important inputs in Ph.D. projects, which can have long-lasting effects on academic careers. Moreover, having multiple supervisors exposes one to a diversity of inputs. We show that Ph.D. students benefit from having multiple supervisors instead of a single one. The cognitive diversity of mentors has a subtler effect in that it increases both the likelihood of success (having many mentees later on) and failure (having no mentees at all later on). We understand the effect of diverse mentorship as a high-risk, high-gain strategy: the recombination of unrelated expertise often fails, but sometimes leads to true novelty.
Effects of fitness qigong and tai chi on middle-aged and elderly patients with type 2 diabetes mellitus
Authors: Li Xiaoyuan , Si Hongyu , Chen Yamin , Li Shouhao , Yin Ningning , Wang Zhenlong
Published: December 2020

Currently, qigong and tai chi exercises are the two most common preventive as well as therapeutic interventions for chronic metabolic diseases such as type 2 diabetes mellitus (T2DM). However, the quantitative evaluation of these interventions is limited. This study aimed to evaluate the therapeutic efficacy of qigong and tai chi intervention in middle-aged and older adults with T2DM. The study included 103 eligible participants, who were randomized to participate for 12 weeks, in one of the following intervention groups for the treatment of T2DM: fitness qigong, tai chi, and control group. Three biochemical measures, including fasting plasma glucose (FPG), glycated hemoglobin (HbA1C), and C-peptide (C-P) levels, assessed at baseline and 12 weeks, served as the primary outcome measures. During the training process, 16 of the 103 participants dropped out. After the 12-week intervention, there were significant influences on HbA1C (F2,83 = 4.88, p = 0.010) and C-P levels (F2,83 = 3.64, p = 0.031). Moreover, significant reduction in C-P levels was observed after 12-week tai chi practice (p = 0.004). Furthermore, there was a significant negative correlation between the duration of T2DM and the relative changes in FPG levels after qigong intervention, and the relative changes in HbA1C levels were positively correlated with waist-to-height ratio after tai chi practice. Our study suggests that targeted qigong exercise might have a better interventional effect on patients with a longer duration of T2DM, while tai chi might be risky for people with central obesity.

Trial registration: This trial was registered in Chinese Clinical Trial Registry. The registration number is ChiCTR180020069. The public title is “Health-care qigong · study for the prescription of chronic diabetes intervention.”
Relationship between high shear stress and OCT-verified thin-cap fibroatheroma in patients with coronary artery disease
Authors: Okamoto Naotaka , Vengrenyuk Yuliya , Fuster Valentin , Samady Habib , Yasumura Keisuke , Baber Usman , Barman Nitin , Suleman Javed , Sweeny Joseph , Krishnan Prakash , Mehran Roxana , Sharma Samin K. , Narula Jagat , Kini Annapoorna S.
Published: December 2020

High-risk coronary plaques have been considered predictive of adverse cardiac events. Both wall shear stress (WSS) in patients with hemodynamically significant lesions and optical coherence tomography (OCT) -verified thin-cap fibroatheroma (TCFA) are associated with plaque rupture, the most common underlying mechanism of acute coronary syndrome. The aim of the study was to test the hypothesis that invasive coronary angiography-based high WSS is associated with the presence of TCFA detected by OCT in obstructive lesions. From a prospective study of patients who underwent OCT examination for angiographically obstructive lesions (Yellow II), we selected patients who had two angiographic projections to create a 3-dimensional reconstruction model to allow assessment of WSS. The patients were divided into 2 groups according to the presence and absence of TCFA. Mean WSS was assessed in the whole lesion and in the proximal, middle and distal segments. Of 70 patients, TCFA was observed in 13 (19%) patients. WSS in the proximal segment (WSSproximal) (10.20 [5.01, 16.93Pa]) and the whole lesion (WSSlesion) (12.37 [6.36, 14.55Pa]) were significantly higher in lesions with TCFA compared to WSSproximal (5.84 [3.74, 8.29Pa], p = 0.02) and WSSlesion (6.95 [4.41, 11.60], p = 0.04) in lesions without TCFA. After multivariate analysis, WSSproximal was independently associated with the presence of TCFA (Odds ratio 1.105; 95%CI 1.007–1.213, p = 0.04). The optimal cutoff value of WSSproximal to predict TCFA was 6.79 Pa (AUC: 0.71; sensitivity: 0.77; specificity: 0.63 p = 0.02). Our results demonstrate that high WSS in the proximal segments of obstructive lesions is an independent predictor of OCT-verified TCFA.
De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing
Authors: Yan Dongqing , Ren Jing , Liu Jiamei , Ding Yu , Niu Jianming
Published: December 2020

Grassland is one of the most widely-distributed ecosystems on Earth and provides a variety of ecosystem services. Grasslands, however, currently suffer from severe degradation induced by human activities, overgrazing pressure and climate change. In the present study, we explored the transcriptome response of Stipa breviflora, a dominant species in the desert steppe, to grazing through transcriptome sequencing, the development of simple sequence repeat (SSR) markers, and analysis of genetic diversity. De novo assembly produced 111,018 unigenes, of which 88,164 (79.41%) unigenes were annotated. A total of 686 unigenes showed significantly different expression under grazing, including 304 and 382 that were upregulated and downregulated, respectively. These differentially expressed genes (DEGs) were significantly enriched in the “alpha-linolenic acid metabolism” and “plant-pathogen interaction” pathways. Based on transcriptome sequencing data, we developed eight SSR molecular markers and investigated the genetic diversity of S. breviflora in grazed and ungrazed sites. We found that a relatively high level of S. breviflora genetic diversity occurred under grazing. The findings of genes that improve resistance to grazing are helpful for the restoration, conservation, and management of desert steppe.
Physiological and transcriptomic analyses of yellow horn (Xanthoceras sorbifolia) provide important insights into salt and saline-alkali stress tolerance
Authors: Wang Juan , Zhang Yunxiang , Yan Xingrong , Guo Jinping
Published: December 2020

Yellow horn (Xanthoceras sorbifolia) is an oil-rich woody plant cultivated for bio-energy production in China. Soil saline-alkalization is a prominent agricultural-related environmental problem limiting plant growth and productivity. In this study, we performed comparative physiological and transcriptomic analyses to examine the mechanisms of X. sorbifolia seedling responding to salt and alkaline-salt stress. With the exception of chlorophyll content, physiological experiments revealed significant increases in all assessed indices in response to salt and saline-alkali treatments. Notably, compared with salt stress, we observed more pronounced changes in electrolyte leakage (EL) and malondialdehyde (MDA) levels in response to saline-alkali stress, which may contribute to the greater toxicity of saline-alkali soils. In total, 3,087 and 2,715 genes were differentially expressed in response to salt and saline-alkali treatments, respectively, among which carbon metabolism, biosynthesis of amino acids, starch and sucrose metabolism, and reactive oxygen species signaling networks were extensively enriched, and transcription factor families of bHLH, C2H2, bZIP, NAC, and ERF were transcriptionally activated. Moreover, relative to salt stress, saline-alkali stress activated more significant upregulation of genes related to H+ transport, indicating that regulation of intracellular pH may play an important role in coping with saline-alkali stress. These findings provide new insights for investigating the physiological changes and molecular mechanisms underlying the responses of X. sorbifolia to salt and saline-alkali stress.
Back loading estimation during team handling: Is the use of only motion data sufficient?
Authors: Muller Antoine , Corbeil Philippe
Published: December 2020

Analyzing back loading during team manual handling tasks requires the measurement of external contacts and is thus limited to standardized tasks. This paper evaluates the possibility of estimating L5/S1 joint moments based solely on motion data. Ten subjects constituted five two-person teams and handling tasks were analyzed with four different box configurations. Three prediction methods for estimating L5/S1 joint moments were evaluated by comparing them to a gold standard using force platforms: one used only motion data, another used motion data and the traction/compression force applied to the box and one used motion data and the ground reaction forces of one team member. The three prediction methods were based on a contact model with an optimization-based method. Using only motion data did not allow an accurate estimate due to the traction/compression force applied by each team member, which affected L5/S1 joint moments. Back loading can be estimated using motion data and the measurement of the traction/compression force with relatively small errors, comparable to the uncertainty levels reported in other studies. The traction/compression force can be obtained directly with a force measurement unit built into the object to be moved or indirectly by using force platforms on which one of the two handlers stands during the handling task. The use of the proposed prediction methods allows team manual handling tasks to be analyzed in various realistic contexts, with team members who have different anthropometric measurements and with different box characteristics.
Video modelling technique used to manage the behaviour of uncooperative children in a dental set up
Authors: Kotian Niharika , Subramanian Erulappan Muthu Ganapathi , Ravindran Vignesh
Published: December 2020

Objective: To investigate if video modelling is an effective technique in behaviour modelling of a child in a dental set up. Material and Methods: Fifty children aged 4-6 years indicated for pulpectomy were enrolled in this study.  They were selected based on their behaviour using Frankl behaviour rating scale.  Children with Frankl behaviour rating 1 and 2 were selected for this study. The video of a child who was cooperative while undergoing pulpectomy was shown to these children. The behaviour was assessed using Frankl behaviour rating scale after the video was shown to the children included in the study. Results: There was a statistically significant difference in the behaviour rating score of the children before after the video of the cooperative child was shown to them. Conclusion: It was observed that video modelling is an effective technique in managing the behaviour of an uncooperative child in a dental set up.
Keywords
Video modelling; Behaviour management; Fear; Anxiety; Pediatric dentistry.
Color Stability of Bulk Fill Composite Resins Submitted to Coffee Staining
Authors: Arruda Beatriz Maria , Bassi Julio Cesar , Vitti Rafael Pino , Scatolin Renata Siqueira
Published: December 2020

Objective: The aim of this in vitro study was to evaluate the color stability of different bulk fill composite resins after extrinsic staining with coffee. Material and methods: Forty-eight disk-shaped specimens (6 mm diameter x 2 mm thick) of each composite resin were prepared for color change test. The composite resins were divided into 4 groups (n=12): one conventional composite resin (Filtek Z250) and three bulk-fill composite resins (Filtek Bulk Fill, Aura Bulk Fill, and Opus Bulk Fill). The samples were stained with 20 mL coffee solution for 7 days, with the solution being replaced every 24 h. The color change analysis was performed using a spectrophotometer, according to the CIE Lab formula. Data were analyzed using the Analysis of variance (ANOVA) and Tukey HSD tests (?=0.05). Results: The results revealed significant differences in color change among the composite resins tested (?<0.0001). The Aura Bulk Fill and Opus Bulk Fill showed the highest color change values. Conclusions: Thus, all the resins analyzed were susceptible to extrinsic staining with coffee, and the Filtek Bulk Fill showed the lowest color change value.
O Livro de Artista: potencialidades pedagógicas no ensino do desenho
Authors: Regatão José
Published: December 2020

O livro de artista é uma modalidade de expressão que tem crescido de popularidade entre artistas plásticos que reconheceram as potencialidades expressivas deste suporte para a criação artística.  Neste artigo pretendemos apresentar os aspetos pedagógicos da utilização do livro de artista no ensino da disciplina de desenho, refletindo sobre a utilização deste instrumento no âmbito da educação artística e analisando os resultados da sua aprendizagem. Enquanto espaço experimental por natureza, o livro de artista permite uma grande diversidade de abordagens e formatos, sendo por esse motivo um suporte ideal para promover a aprendizagem de diferentes técnicas e linguagens do desenho. Concluiu-se que este projeto promoveu uma abordagem experimental ao desenho, incentivou a pesquisa e a mobilização de conceitos, tendo proporcionado a aquisição de diversas competências técnicas e visuais.