bluuebunny/crossref_metadata_2025 huggingface dataset:
Lay social workers implementing a task-sharing approach to managing depression in Vietnam
Authors: Chau Leena W. , Murphy Jill , Nguyen Vu Cong , Lou Hayami , Khanh Huyen , Thu Trang , Minas Harry , O’Neil John
Published: July 2021

Background

While depression is a leading contributor to burden of disease in Vietnam, there is a critical gap in depression care due to the shortage of mental health specialists and extremely limited mental health services in general health care settings. We have previously reported the effectiveness of a supported self-management (SSM) task-sharing intervention for depression, delivered by social collaborators (lay social workers). The purpose of this study was to identify factors influencing the effectiveness of delivery of SSM by social collaborators and delineate areas for further attention that are relevant for scale-up.

Methods

A hundred and ten (110) key informant interviews were conducted with three stakeholder groups (patients, social collaborators, experts) from eight provinces in Vietnam. Participants were identified through records from a recently completed randomized trial that showed the effectiveness of SSM in community-based settings in Vietnam. Qualitative descriptive methods and thematic analysis were used to examine the interviews. A coding framework and corresponding themes were developed deductively, based on the findings from the randomized trial and the literature, and through inductive analysis, to describe the contextual factors that impacted the social collaborators’ role in successfully implementing the SSM intervention.

Results

Our analysis identified the following benefits of working with social collaborators: (1) increased awareness of mental health in the family and community; (2) reduced stigma; (3) a better understanding that depression is treatable; (4) increased help-seeking; and (5) improved access to care. There were also significant challenges, including social collaborator characteristics (age, education, pre-existing training and skills) and contextual factors influencing their work (roles and responsibilities, training, compensation, support from government).

Conclusions

Engaging social collaborators in the delivery of SSM in the community can help fill a critical gap in depression care in Vietnam. However, several contextual challenges that are an impediment to increased engagement and sustainable integration into health and social systems need to be resolved through policy change to regulate their practice, define their scope of work, and provide adequate remuneration.
Precision oncology in AML: validation of the prognostic value of the knowledge bank approach and suggestions for improvement
Authors: Bill Marius , Mrózek Krzysztof , Giacopelli Brian , Kohlschmidt Jessica , Nicolet Deedra , Papaioannou Dimitrios , Eisfeld Ann-Kathrin , Kolitz Jonathan E. , Powell Bayard L. , Carroll Andrew J. , Stone Richard M. , Garzon Ramiro , Byrd John C. , Bloomfield Clara D. , Oakes Christopher C.
Published: July 2021

Recently, a novel knowledge bank (KB) approach to predict outcomes of individual patients with acute myeloid leukemia (AML) was developed using unbiased machine learning. To validate its prognostic value, we analyzed 1612 adults with de novo AML treated on Cancer and Leukemia Group B front-line trials who had pretreatment clinical, cytogenetics, and mutation data on 81 leukemia/cancer-associated genes available. We used receiver operating characteristic (ROC) curves and the area under the curve (AUC) to evaluate the predictive values of the KB algorithm and other risk classifications. The KB algorithm predicted 3-year overall survival (OS) probability in the entire patient cohort (AUCKB = 0.799), and both younger (< 60 years) (AUCKB = 0.747) and older patients (AUCKB = 0.770). The KB algorithm predicted non-remission death (AUCKB = 0.860) well but was less accurate in predicting relapse death (AUCKB = 0.695) and death in first complete remission (AUCKB = 0.603). The KB algorithm’s 3-year OS predictive value was higher than that of the 2017 European LeukemiaNet (ELN) classification (AUC2017ELN = 0.707, p < 0.001) and 2010 ELN classification (AUC2010ELN = 0.721, p < 0.001) but did not differ significantly from that of the 17-gene stemness score (AUC17-gene = 0.732, p = 0.10). Analysis of additional cytogenetic and molecular markers not included in the KB algorithm revealed that taking into account atypical complex karyotype, infrequent recurrent balanced chromosome rearrangements and mutational status of the SAMHD1, AXL and NOTCH1 genes may improve the KB algorithm. We conclude that the KB algorithm has a high predictive value that is higher than those of the 2017 and 2010 ELN classifications. Inclusion of additional genetic features might refine the KB algorithm.
TRAFIC: statistical design and analysis plan for a pragmatic early phase 1/2 Bayesian adaptive dose escalation trial in rheumatoid arthritis
Authors: Cole M. , Yap C. , Buckley C. , Ng W. F. , McInnes I. , Filer A. , Siebert S. , Pratt A. , Isaacs J. D. , Stocken D. D.
Published: July 2021

Background

Adaptive model-based dose-finding designs have demonstrated advantages over traditional rule-based designs but have increased statistical complexity but uptake has been slow especially outside of cancer trials. TRAFIC is a multi-centre, early phase trial in rheumatoid arthritis incorporating a model-based design.

Methods

A Bayesian adaptive dose-finding phase I trial rolling into a single-arm, single-stage phase II trial. Model parameters for phase I were chosen via Monte Carlo simulation evaluating objective performance measures under clinically relevant scenarios and incorporated stopping rules for early termination. Potential designs were further calibrated utilising dose transition pathways.

Discussion

TRAFIC is an MRC-funded trial of a re-purposed treatment demonstrating that it is possible to design, fund and implement a model-based phase I trial in a non-cancer population within conventional research funding tracks and regulatory constraints. The phase I design allows borrowing of information from previous trials, all accumulated data to be utilised in decision-making, verification of operating characteristics through simulation, improved understanding for management and oversight teams through dose transition pathways. The rolling phase II design brings efficiencies in trial conduct including site and monitoring activities and cost.

TRAFIC is the first funded model-based dose-finding trial in inflammatory disease demonstrating that small phase I/II trials can have an underlying statistical basis for decision-making and interpretation.

Trial registration

Trials Registration: ISRCTN,ISRCTN36667085. Registered on September 26, 2014.
Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review
Authors: Sethupathy Sivasamy , Morales Gabriel Murillo , Li Yixuan , Wang Yongli , Jiang Jianxiong , Sun Jianzhong , Zhu Daochen
Published: July 2021

The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem functioning. Therefore, the use of microbes is an attractive way to transform biomass to produce clean energy and high-value compounds. The microbial degradation of lignocelluloses is a complex process which is dependent upon multiple secreted enzymes and their synergistic activities. The availability of the cutting edge proteomics and highly sensitive mass spectrometry tools make possible for researchers to probe the secretome of microbes and microbial consortia grown on different lignocelluloses for the identification of hydrolytic enzymes of industrial interest and their substrate-dependent expression. This review summarizes the role of secretomics in identifying enzymes involved in lignocelluloses deconstruction, the development of enzyme cocktails and the construction of synthetic microbial consortia for biomass valorization, providing our perspectives to address the current challenges.
Correction to: First report of Theileria annulata in Nigeria: Findings from cattle ticks in Zamfara and Sokoto States
Authors: Mamman Adamu Haruna , Lorusso Vincenzo , Adam Babagana Mohammed , Dogo Goni Abraham , Bown Kevin J. , Birtles Richard J.
Published: July 2021

An amendment to this paper has been published and can be accessed via the original article.
A global overview of genetically interpretable multimorbidities among common diseases in the UK Biobank
Authors: Dong Guiying , Feng Jianfeng , Sun Fengzhu , Chen Jingqi , Zhao Xing-Ming
Published: July 2021

Background

Multimorbidities greatly increase the global health burdens, but the landscapes of their genetic risks have not been systematically investigated.

Methods

We used the hospital inpatient data of 385,335 patients in the UK Biobank to investigate the multimorbid relations among 439 common diseases. Post-GWAS analyses were performed to identify multimorbidity shared genetic risks at the genomic loci, network, as well as overall genetic architecture levels. We conducted network decomposition for the networks of genetically interpretable multimorbidities to detect the hub diseases and the involved molecules and functions in each module.

Results

In total, 11,285 multimorbidities among 439 common diseases were identified, and 46% of them were genetically interpretable at the loci, network, or overall genetic architecture levels. Multimorbidities affecting the same and different physiological systems displayed different patterns of the shared genetic components, with the former more likely to share loci-level genetic components while the latter more likely to share network-level genetic components. Moreover, both the loci- and network-level genetic components shared by multimorbidities converged on cell immunity, protein metabolism, and gene silencing. Furthermore, we found that the genetically interpretable multimorbidities tend to form network modules, mediated by hub diseases and featuring physiological categories. Finally, we showcased how hub diseases mediating the multimorbidity modules could help provide useful insights for the genetic contributors of multimorbidities.

Conclusions

Our results provide a systematic resource for understanding the genetic predispositions of multimorbidities and indicate that hub diseases and converged molecules and functions may be the key for treating multimorbidities. We have created an online database that facilitates researchers and physicians to browse, search, or download these multimorbidities (https://multimorbidity.comp-sysbio.org).
The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients
Authors: Wangriatisak Kittikorn , Thanadetsuntorn Chokchai , Krittayapoositpot Thamonwan , Leepiyasakulchai Chaniya , Suangtamai Thanitta , Ngamjanyaporn Pintip , Khowawisetsut Ladawan , Khaenam Prasong , Setthaudom Chavachol , Pisitkun Prapaporn , Chootong Patchanee
Published: July 2021

Background

Autoreactive B cells are well recognized as key participants in the pathogenesis of systemic lupus erythematosus (SLE). However, elucidating the particular subset of B cells in producing anti-dsDNA antibodies is limited due to their B cell heterogeneity. This study aimed to identify peripheral B cell subpopulations that display autoreactivity to DNA and contribute to lupus pathogenesis.

Methods

Flow cytometry was used to detect total B cell subsets (n = 20) and DNA autoreactive B cells (n = 15) in SLE patients’ peripheral blood. Clinical disease activities were assessed in SLE patients using modified SLEDAI-2 K and used for correlation analyses with expanded B cell subsets and DNA autoreactive B cells.

Results

The increases of circulating double negative 2 (DN2) and activated naïve (aNAV) B cells were significantly observed in SLE patients. Expanded B cell subsets and DNA autoreactive B cells represented a high proportion of aNAV B cells with overexpression of CD69 and CD86. The frequencies of aNAV B cells in total B cell populations were significantly correlated with modified SLEDAI-2 K scores. Further analysis showed that expansion of aNAV DNA autoreactive B cells was more related to disease activity and serum anti-dsDNA antibody levels than to total aNAV B cells.

Conclusion

Our study demonstrated an expansion of aNAV B cells in SLE patients. The association between the frequency of aNAV B cells and disease activity patients suggested that these expanded B cells may play a role in SLE pathogenesis.
Development of a novel selective medium for culture of Gram-negative bacteria
Authors: Al-blooshi Shooq Yousef , Latif Mustafa Amir Abdul , Sabaneh Nour K. , Mgaogao Michael , Hossain Ashfaque
Published: July 2021

Objective

Although many bacterial culture media are available commercially, there is a continuous effort to develop better selective media for bacteria, which cannot be grown on existing media. While exploring antibacterial properties of clove, we observed that it has the potential to selectively inhibit growth of certain types of bacteria. This led us to do the experiments, which resulted in developing a new media which selectively allowed the growth of only Gram-negative bacteria, while inhibiting the Gram-positive bacteria.

Results

Mueller Hinton Agar (MHA) was used as the base media and was modified to develop MHA-C15 (MHA containing 15% volume/volume water extract of clove). Gram-negative bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium and Pseudomonas aeruginosa grew on MHA-C15. However, none of the major Gram-positive bacterial pathogens such as Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus mutans, Bacillus spp. and Enterococcus spp. grew on it. Taken together, these findings show that MHA-C15 is a newly developed selective media for culture of Gram-negative bacteria.
Sex difference in the association between plasma selenium and first stroke: a community-based nested case-control study
Authors: Hu Huan , Bi Chonglei , Lin Tengfei , Liu Lishun , Song Yun , Wang Binyan , Wang Ping , Zhou Ziyi , Fang Chongqian , Ma Hai , Huang Xiao , Hu Lihua , Xu Xiping , Zhang Hao , Huo Yong , Wang Xiaobin , Bao Huihui , Cheng Xiaoshu , Li Ping
Published: July 2021

Background

To date, there is no clearly defined association between plasma selenium levels and first stroke. We aimed to investigate the association between baseline plasma selenium and first stroke risk in a community-based Chinese population.

Methods

Using a nested case-control study design, a total of 1255 first stroke cases and 1255 matched controls were analyzed. Participant plasma selenium concentrations were measured by inductively coupled plasma mass spectrometry (ICP-MS), and the association of plasma selenium with first stroke risk was estimated by conditional logistic regression models.

Results

Overall, a non-linear negative association between plasma selenium and first total stroke and first ischemic stroke risks was found in males but not in females. Compared with participants with lower selenium levels (tertile 1–2, < 94.1 ng/mL), participants with higher selenium levels (tertile 3, ≥ 94.1 ng/mL) had significantly lower risks of first total stroke (OR 0.63; 95% CI 0.48, 0.83) and first ischemic stroke (OR 0.61; 95% CI 0.45, 0.83) in males but not in females with first total stroke (OR 0.92; 95% CI 0.69, 1.22) and first ischemic stroke (OR 0.89; 95% CI 0.65, 1.22). Furthermore, a stronger association between plasma selenium and first total stroke was found in males with higher vitamin E levels (≥ 13.5 μg/mL vs. < 13.5 μg/mL P-interaction = 0.007). No significant association was observed between plasma selenium and first hemorrhagic stroke risk in either males or females.

Conclusion

Our study indicated a significant, non-linear, negative association between plasma selenium and first stroke in males but not in females.

Trial registration

ChiCTR1800017274.
Factors associated with childhood diarrheal in Ethiopia; a multilevel analysis
Authors: Fenta Setegn Muche , Nigussie Teshager Zerihun
Published: July 2021

Background

Diarrhea is the second cause of child deaths globally. According to World Health Organization reports, in each year it kills more than 525,000 children under-5 years. More than half of these deaths occur in five countries including Ethiopia. This study aimed to identify both individual and community-level risk factors of childhood diarrheal in Ethiopia.

Methods

Ethiopian demography and health survey of 2016 data were used for the analysis. A total of 10,641 children aged 0–59 months were included in the analysis. A multi-level mixed-effect logistic regression model was used to identify both individual and community-level risk factors associated with childhood diarrheal.

Result

The incidence of childhood diarrheal was 12% (95%CI: 11.39, 12.63). The random effect model revealed that 67% of the variability of childhood diarrhea explained by individual and community level factors. From the individual-level factors, children aged 36–59 month (AOR = 3.166; 95% CI: 2.569, 3.900), twin child (AOR = 1.871; 95% CI: 1.390, 2.527), birth order 5 and above (AOR = 2.210, 95% CI: 1.721, 2.839), not received any vaccination (AOR = 1.197; 95% CI: 1.190,1.527), smaller size of child at birth (AOR = 1.303;95% CI: 1.130,1.504) and never breastfed children (AOR = 2.91;95%CI:2.380,3.567) associated with the higher incidence of childhood diarrhea. From the community-level factors, living in a rural area ((AOR = 1.505; 95%CI: 1.233, 1.836)), unprotected source of drinking water (AOR: 1.289; 95% CI: 1.060, 1.567) and availability of unimproved latrine facilities (OR: 1.289; 95% CI: 1.239, 1.759) associated with the higher incidence of childhood diarrhea. Besides, Children live in Afar, Amhara, Benishangul-Gumuz, Gambella, SNNPR and Dire Dawa regions had higher incidence of childhood diarrhea.

Conclusion

The incidence of childhood diarrhea was different from cluster to clusters in Ethiopia. Therefore, integrated child health intervention programs including provisions of toilet facility, access to a clean source of drinking water, educate parents about the importance of breastfeeding and vaccination have to be strongly implemented in order to reduce the high incidence of childhood diarrhea among children in Ethiopia.
Should screening for risk of gambling-related harm be undertaken in health, care and support settings? A systematic review of the international evidence
Authors: Blank Lindsay , Baxter Susan , Woods Helen Buckley , Goyder Elizabeth
Published: July 2021

Background

Gambling-related harm is an increasing recognised problem internationally. Recent years have seen an explosion in opportunities to gamble, both in person and online. Health and other care settings have the potential to act as screening sites to identify and support gamblers who may be at high risk of experiencing gambling-related harm. This study aimed to identify interventions to screen for risk of gambling-related harm in the general population which may be delivered in health, care and support settings.

Methods

Systematic review. Searches of key databases and grey sources since 2012 were undertaken in October 2019. Electronic database searches generated a total of 5826 unique hits. Nine studies published 2013–2019, along with thirteen grey literature documents met our eligibility criteria. The criteria were setting (health, care and support settings), participants (any attendee in help, care and support settings), interventions (screening to identify risk of harm from gambling behaviours) and outcome measures (gambling behaviours, service use).

Results

Three papers evaluating screening interventions delivered in general practice (repeat visits and written advice), mental health service (the use of screening tools to identify risk of harm), and substance abuse treatment (intensive outpatient treatment for substance use disorders or methadone maintenance) indicated evidence of potential effectiveness. Six papers supported the feasibility and acceptability of delivering interventions in various settings. Grey literature reports described the implementation of interventions such as training materials, and transfer of interventions developed for substance abuse populations by practitioners.

Conclusions

Health, care and support services offer potentially important contexts in which to identify and offer support to people who are at risk of gambling related harm. Screening interventions appear feasible and acceptable in a range of community and healthcare settings for those at risk of gambling harm. Evaluation of effectiveness and cost-effectiveness of screening in these populations should therefore be prioritised.
Study on clinical nursing pathway to promote the effective implementation of sepsis bundle in septic shock
Authors: Liu Chun-Xia , Wang Xian-Lei , Zhang Kun , Hao Gui-Zhen , Han Wei-Yan , Tian Yi-Qing , Ge Lan , Shen Li-Min
Published: July 2021

Background

There is still a certain gap between the effective implementation and requirements of sepsis bundle. Our aim is to establish the clinical nursing pathway of the cluster treatment of septic shock in the Intensive Care Unit and promote effective implementation of the cluster treatment of septic shock.

Methods

By means of evidence-based method, quality control index requirements and on-site investigation, the implementation process of clinical nursing pathway of the cluster treatment within 6 h of diagnosis of septic shock was established.

Results

After the implementation of clinical nursing pathway, the completion rate of septic shock cluster treatment was 81.4% (66.4%) in 1 h, 89.4% (77.0%) in 3 h, 95.5% (82.3%) in 6 h (P < 0.05), which was significantly improved in the experimental group compared with the control group.

Conclusions

The clinical nursing pathway of septic shock cluster treatment is guided by evidence-based nursing, which emphasizes standardization and standardization of septic shock cluster treatment nursing under the guidance of the guideline, and can promote the effective implementation of septic shock cluster treatment, significantly improve efficiency of septic shock treatment and the quality of medical care.
Prediction of chemoresistance trait of cancer cell lines using machine learning algorithms and systems biology analysis
Authors: Ataei Atousa , Majidi Niloufar Seyed , Zahiri Javad , Rostami Mehrdad , Arab S. Shahriar , Rizvanov Albert A.
Published: July 2021

Most of the current cancer treatment approaches are invasive along with a broad spectrum of side effects. Furthermore, cancer drug resistance known as chemoresistance is a huge obstacle during treatment. This study aims to predict the resistance of several cancer cell-lines to a drug known as Cisplatin. In this papers the NCBI GEO database was used to obtain data and then the harvested data was normalized and its batch effects were corrected by the Combat software. In order to select the appropriate features for machine learning, the feature selection/reduction was performed based on the Fisher Score method. Six different algorithms were then used as machine learning algorithms to detect Cisplatin resistant and sensitive samples in cancer cell lines. Moreover, Differentially Expressed Genes (DEGs) between all the sensitive and resistance samples were harvested. The selected genes were enriched in biological pathways by the enrichr database. Topological analysis was then performed on the constructed networks using Cytoscape software. Finally, the biological description of the output genes from the performed analyses was investigated through literature review. Among the six classifiers which were trained to distinguish between cisplatin resistance samples and the sensitive ones, the KNN and the Naïve Bayes algorithms were proposed as the most convenient machines according to some calculated measures. Furthermore, the results of the systems biology analysis determined several potential chemoresistance genes among which PTGER3, YWHAH, CTNNB1, ANKRD50, EDNRB, ACSL6, IFNG and, CTNNB1 are topologically more important than others. These predictions pave the way for further experimental researches.
In vitro antibacterial effect of fifth generation dentin bonding agent incorporated with nisin on Streptococcus mutans
Authors: Keerthipriya Gopal , Ravikumar Nesamani , Mahalaxmi Sekar
Published: July 2021

Introduction

Bacterial adherence to restorative materials such as composite resin is one of the aetiology of secondary caries. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with nisin, against Streptococcus mutans based on its growth, adherence and membrane integrity.

Methods

Adhesive eluents of the experimental bonding agents were obtained using 250 μl Brain Heart Infusion (BHI) broth and the groups were control (BA with 0% Nisin), bonding agent with 1 wt% (NBA 1) and 5 wt% nisin (NBA 5). To this, 10 μl S. mutans culture was added and incubated at 37 °C. Bacterial growth was estimated by changes in optical density using spectrophotometer every 20 min for 2 h. The results were statistically analysed using one way ANOVA followed by Tukey Post Hoc test. For adherence and membrane integrity test, 10 μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 h. After rinsing, 1 ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under confocal laser scanning microscope (CLSM) for intact (green/live) and damaged (red/dead) bacterial membranes.

Results

Mean optical density was significantly higher in control group at all time intervals with maximum value at 2 h (0.83 ± 0.008), while there was a concentration dependant reduction in bacterial growth with the NBA groups (0.50 ± 0.007). Correspondingly, the NBA groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group.

Significance

Addition of an antibacterial agent nisin in dentin bonding agent may render the resin dentin interface more resistant to bacterial penetration, and adherence of cariogenic bacteria like S. mutans.
Differentiation of renal angiomyolipoma without visible fat from small clear cell renal cell carcinoma by using specific region of interest on contrast-enhanced CT: a new combination of quantitative tools
Authors: Wang Xu , Song Ge , Jiang Haitao
Published: July 2021

Background

To investigate the value of using specific region of interest (ROI) on contrast-enhanced CT for differentiating renal angiomyolipoma without visible fat (AML.wovf) from small clear cell renal cell carcinoma (ccRCC).

Methods

Four-phase (pre-contrast phase [PCP], corticomedullary phase [CMP], nephrographic phase [NP], and excretory phase [EP]) contrast-enhanced CT images of AML.wovf (n = 31) and ccRCC (n = 74) confirmed by histopathology were retrospectively analyzed. The CT attenuation value of tumor (AVT), net enhancement value (NEV), relative enhancement ratio (RER), heterogeneous degree of tumor (HDT) and standardized heterogeneous ratio (SHR) were obtained by using different ROIs [small: ROI (1), smaller: ROI (2), large: ROI (3)], and the differences of these quantitative data between AML.wovf and ccRCC were statistically analyzed. Multivariate regression was used to screen the main factors for differentiation in each scanning phase, and the prediction models were established and evaluated.

Results

Among the quantitative parameters determined by different ROIs, the degree of enhancement measured by ROI (2) and the enhanced heterogeneity measured by ROI (3) performed better than ROI (1) in distinguishing AML.wovf from ccRCC. The receiver operating characteristic (ROC) curves showed that the area under the curve (AUC) of RER_CMP (2), RER_NP (2) measured by ROI (2) and HDT_CMP and SHR_CMP measured by ROI (3) were higher (AUC = 0.876, 0.849, 0.837 and 0.800). Prediction models that incorporated demographic data, morphological features and quantitative data derived from the enhanced phase were superior to quantitative data derived from the pre-contrast phase in differentiating between AML.wovf and ccRCC. Among them, the model in CMP was the best prediction model with the highest AUC (AUC = 0.986).

Conclusion

The combination of quantitative data obtained by specific ROI in CMP can be used as a simple quantitative tool to distinguish AML.wovf from ccRCC, which has a high diagnostic value after combining demographic data and morphological features.
The headcam mother-infant interaction assessment tool: testing the feasibility and acceptability in Soweto, South Africa, using participatory engagement
Authors: Wrottesley Stephanie V. , Pearson Rebecca , Prioreschi Alessandra
Published: July 2021

Background

Many children in low- and middle-income countries lack the stimulation needed to support healthy growth and development. Sensitive interactions between caregivers and infants may promote healthy movement behaviours in infants, which could improve childhood growth and development. However, reliable measures for such interactions require testing in the South African context. The aim of this study was to test the acceptability and feasibility of the headcam caregiver-infant interaction assessment tool in mothers from Soweto, South Africa.

Methods

Nineteen mother and infant (6–24 months) pairs were asked to wear headcams (first-person observation) while participating in group and individual activities. Detailed instructions on headcam use were provided before and during these activities. Mothers were then asked to use the headcams, as well as photoframe cameras (which provided context of the interactions), in at least three, 5-min mother-infant engagement sessions at home over a 1-week period. Thereafter, focus group discussions (FGDs) were conducted to explore mothers’ experiences of using the tool in the home setting. The feasibility of the headcam mother-infant interaction tool was assessed according to a priori criteria which scored (i) technical reliability of the devices and (ii) usability of the recorded footage. Acceptability was assessed according to emerging themes which were coded from the FGDs using a constant comparison method by two researchers.

Results

The headcam mother-infant assessment tool was found to be feasible in Soweto, and sufficient data was available to code. Three main themes emerged from the FGD analysis: use of the headcam, using the headcams in the home environment and using the photoframe vs. the headcam. Mothers remarked on the ease of using the tool across daily activities, the normality of their infant’s behaviour during recording and the acceptability by other members of the household. Large amounts of wasted unusable recordings were produced, and challenges related to switching the cameras on and off and to headcam placement were discussed.

Conclusions

Our study shows that headcams are both an acceptable and feasible method for assessing mother-infant interactions in Soweto. However, improvements to the usability of the tool and the quality of the data collected should be made prior to future work.
Analysing the behavioural finance impact of 'fake news' phenomena on financial markets: a representative agent model and empirical validation
Authors: Fong Bryan
Published: July 2021

This paper proposes an original behavioural finance representative agent model, to explain how fake news’ empirical price impacts can persist in finance despite contradicting the efficient-market hypothesis. The model reconciles empirically-observed price overreactions to fake news with empirically-observed price underreactions to real news, and predicts a novel secondary impact of fake news: that fake news in a security amplifies underreactions to subsequent real news for the security. Evaluating the model against a large-sample event study of the 2019 Chinese ADR Delisting Threat fake news and debunking event, this paper finds strong qualitative validation for its model’s dynamics and predictions.
Empowering Maasai women behind the camera: Photovoice as a tool for trachoma control
Authors: Mtuy Tara B. , Mepukori Jeremiah , Lankoi Joseph , Lees Shelley
Published: July 2021

Background

Photovoice is a method used to help engage community members to understand local realities and promote social change. Photovoice uses cameras in the hands of participants as a tool to visually document a specified topic. Photos taken by participants allow for reflection and stimulate dialog on an issue to ideally lead to social change. Trachoma, hyperendemic in Maasai communities in Northern Tanzania, is the commonest infectious cause of blindness worldwide, caused by chlamydia trachomatis. The bacterial infection commonly occurs in childhood and over many years repeated infections leads to inflammation and scarring of the eyelid. Often as adults this leads to the upper eyelid turning inward and eyelashes scratching the eye, resulting in pain and eventually blindness. We used photovoice as a tool for Maasai women to share their lived experiences of educating peers on trachoma and ultimately empowering women in this society.

Methods

This public engagement intervention was conducted September thru October 2017. We held a workshop on trachoma control for 20 Maasai women including use of photovoice method. Women were asked to disseminate information from the trachoma control workshop to their community and to capture their experiences using disposable cameras. Five weeks post-workshop we facilitated a discussion and women displayed photos of the successes and challenges they encountered as advocates for trachoma control in their community.

Intervention Outcomes

It was observed throughout the process and at the photo discussion meeting, that women articulated empowerment by this experience; as educators, agents of change and a source of valued information.

Conclusion

Photovoice should be considered for future interventions as a communication tool on health issues and to empower women to be ambassadors for health promotion.
The use of a pedicled buccal fat pad for reconstruction of posterior mandibular defects
Authors: Lee Hyen Woo , Hong Sung ok , Bae Heeyeon , Shin Youngjin , Jee Yu-jin
Published: July 2021

Background

The pedicled buccal fat pad has been used for a long time to reconstruct oral defects due to its ease of flap formation and few complications. Many cases related to reconstruction of defects in the maxilla, such as closing the oroantral fistula, have been reported, but cases related to the reconstruction of defects in the mandible are limited. Under adequate anterior traction, pedicled buccal fat pad can be a reliable and effective method for reconstruction of surgical defects in the posterior mandible.

Case presentation

This study describes two cases of reconstruction of surgical oral defects in the posterior mandible, all of which were covered by a pedicled buccal fat pad. The size of the flap was sufficient to perfectly close the defect without any tension. Photographic and radiologic imaging showed successful closure of the defects and no problems were noted in the treated area.

Conclusion

In conclusion, the pedicled buccal fat pad graft is a convenient and reliable method for the reconstruction of surgical defects on the posterior mandible.
Characteristic mutations induced in the small intestine of Msh2-knockout gpt delta mice
Authors: Aoki Yasunobu , Ohno Mizuki , Matsumoto Michiyo , Matsumoto Michi , Masumura Kenichi , Nohmi Takehiko , Tsuzuki Teruhisa
Published: July 2021

Background

Base pair mismatches in genomic DNA can result in mutagenesis, and consequently in tumorigenesis. To investigate how mismatch repair deficiency increases mutagenicity under oxidative stress, we examined the type and frequency of mutations arising in the mucosa of the small intestine of mice carrying a reporter gene encoding guanine phosphoribosyltransferase (gpt) and in which the Msh2 gene, which encodes a component of the mismatch repair system, was either intact (Msh2+/+::gpt/0; Msh2-bearing) or homozygously knockout (KO) (Msh2−/−::gpt/0; Msh2-KO).

Results

Gpt mutant frequency in the small intestine of Msh2-KO mice was about 10 times that in Msh2-bearing mice. Mutant frequency in the Msh2-KO mice was not further enhanced by administration of potassium bromate, an oxidative stress inducer, in the drinking water at a dose of 1.5 g/L for 28 days. Mutation analysis showed that the characteristic mutation in the small intestine of the Msh2-KO mice was G-to-A transition, irrespective of whether potassium bromate was administered. Furthermore, administration of potassium bromate induced mutations at specific sites in gpt in the Msh2-KO mice: G-to-A transition was frequently induced at two known sites of spontaneous mutation (nucleotides 110 and 115, CpG sites) and at nucleotides 92 and 113 (3′-side of 5′-GpG-3′), and these sites were confirmed to be mutation hotspots in potassium bromate-administered Msh2-KO mice. Administration of potassium bromate also induced characteristic mutations, mainly single-base deletion and insertion of an adenine residue, in sequences of three to five adenine nucleotides (A-runs) in Msh2-KO mice, and elevated the overall proportion of single-base deletions plus insertions in Msh2-KO mice.

Conclusions

Our previous study revealed that administration of potassium bromate enhanced tumorigenesis in the small intestine of Msh2-KO mice and induced G-to-A transition in the Ctnnb1 gene. Based on our present and previous observations, we propose that oxidative stress under conditions of mismatch repair deficiency accelerates the induction of single-adenine deletions at specific sites in oncogenes, which enhances tumorigenesis in a synergistic manner with G-to-A transition in other oncogenes (e.g., Ctnnb1).
The analysis of sacrum and coccyx length measured with computerized tomography images depending on sex
Authors: Bakici Rukiye Sumeyye , Oner Zulal , Oner Serkan
Published: July 2021

Background

Sex estimation is vital in establishing an accurate biological profile from the human skeleton, as sex influences the analysis of other elements in both Physical and Forensic Anthropology and Legal Medicine. The present study was conducted to analyze the sex differences between the sacrum and coccyx length based on the measurements calculated with computed tomography (CT) images. One hundred case images (50 females, 50 males) who were between the ages of 25 and 50 and admitted by the emergency department between September 2018 and June 2019 and underwent CT were included in the study. Eighteen lengths, 4 curvature lengths, and 2 regions were measured in sagittal, coronal and transverse planes with orthogonal adjustment for three times.

Results

It was stated that the mean anterior and posterior sacral length, anterior and posterior sacrococcygeal length, anterior and posterior sacral curvature length, anterior coccygeal curvature length, sacral area, lengths of transverse lines 1, 2, 3 and 4, sacral first vertebra transverse and sagittal length measurements were longer in males when compared to females (p < 0.05). It was noted that the parameter with the highest discrimination value in the receiver operating characteristic (ROC) analysis was the sacral area (AUC = 0.88/Acc = 0.82). Based on Fisher’s linear discriminant analysis findings, the discrimination rate was 96% for males, 92% for females and the overall discrimination rate was 94%.

Conclusions

It was concluded that the fourteen parameters that were indicated as significant in the present study could be used in anthropology, Forensic Medicine and Anatomy to predict sex.
Modeling the dynamics of Lassa fever in Nigeria
Authors: Ojo Mayowa M. , Gbadamosi B. , Benson Temitope O. , Adebimpe O. , Georgina A. L.
Published: July 2021

Lassa fever is a zoonotic disease spread by infected rodents known as multimammate rats. The disease has posed a significant and major health challenge in West African countries, including Nigeria. To have a deeper understanding of Lassa fever epidemiology in Nigeria, we present a deterministic dynamical model to study its dynamical transmission behavior in the population. To mimic the disease’s biological history, we divide the population into two groups: humans and rodents. We established the quantity known as reproduction number $${\mathcal {R}}_{0}$$

R
0

. The results show that if $${\mathcal {R}}_{0} <1$$

R
0

<
1

then the system is stable, otherwise it is unstable. The model fitting was performed using the nonlinear least square method on cumulative reported cases from Nigeria between 2018 and 2020 to obtain the best fit that describes the dynamics of this disease in Nigeria. In addition, sensitivity analysis was performed, and the numerical solution of the system was derived using an iterative scheme, the fifth-order Runge–Kutta method. Using different numeric values for each parameter, we investigate the effect of all highest sensitivity indices’ parameters on the population of infected humans and infected rodents. Our findings indicate that any control strategies and methods that reduce rodent populations and the risk of transmission from rodents to humans and rodents would aid in the population’s control of Lassa fever.
Values-based practice: how can history taking help psychiatrists explore the values involved in clinical decision-making?
Authors: Dudas Robert B.
Published: July 2021

Values-based practice (VBP) is a framework of clinical theory and skills to facilitate a good process whereby the (often conflicting) values involved in clinical decision-making can be recognised and balanced productively. Many of these values come from the personal histories of the patient and of the clinician, and the traditions and history of psychiatry. New developments in science lead to increasing choice and increasing complexity of values. Therefore, psychiatrists will need more skills in this area, as reflected by the inclusion of VBP in the Royal College of Psychiatrists’ training curricula. This article describes some tools for understanding and navigating this value diversity in applying science to clinical practice during history taking.
Recommendations for monitoring and reporting harm in mindfulness for psychosis research
Authors: Ellett Lyn , Chadwick Paul
Published: July 2021

There is increasing interest in potential harmful effects of mindfulness-based interventions. In relation to psychosis, inconsistency and shortcomings in how harm is monitored and reported are holding back our understanding. We offer eight recommendations to help build a firmer evidence base on potential harm in mindfulness for psychosis.
Descriptive Comparative Analysis of Patients With Cancer Referring to the Emergency Department of an Italian University Hospital Across the Severe Acute Respiratory Syndrome Coronavirus 2 Waves
Authors: Mariniello Annapaola , Bironzo Paolo , Pisano Chiara , De Filippis Marco , Persano Irene , Olmetto Emanuela , Caramello Valeria , Boccuzzi Adriana , Capelletto Enrica , Passiglia Francesco , Di Maio Massimo , Novello Silvia
Published: July 2021

PURPOSE:

COVID-19 cancer patients (C19-CP) represent a population at high risk for mortality, whose clinical characteristics are still unknown in the second SARS-CoV-2 wave. The aim of this retrospective study was to compare epidemiology and clinical presentation of C19-CP referring to the emergency department (ED) of our institution (San Luigi Gonzaga University Hospital, Orbassano, Turin, Italy), in a 3-week observation period of the first and second COVID-19 waves, starting from the introduction of the corresponding national lockdowns.

METHODS:

We retrieved ED admissions from March 9 to 29, 2020, for the first wave, and from October 24 to November 13, 2020, for the second wave. We collected clinical characteristics of consecutive patients with molecularly confirmed SARS-CoV-2 infection. We also considered untested or SARS-CoV-2–negative cancer patients referring to the ED in the reference time frames.

RESULTS:

C19-CP in the second wave exceeded those in the first wave despite the nonsignificant difference (39 of 576 v 8 of 163; P = .5). Compared with nononcological patients, C19-CP were older (median age 70 years [interquartile range 61-77] v 60 years [interquartile range 45-73]; P = .02) and presented more often with ≥ 2 comorbidities (40.4% v 24.3%; P = .02). Compared with nononcological patients, in C19-CP, respiratory failure (29 of 47 v 321 of 692; P = .049) and hospitalization (37 of 47 v 363 of 692; P = .0004) were higher, with comparable frequencies across the waves. Five of 24 and 10 of 27 hospitalized cancer patients in the first and second waves developed SARS-CoV-2 infection during hospitalization.

CONCLUSION:

C19-CP were a vulnerable population, irrespective of the COVID-19 waves. This highlights the need to prioritize vaccinations in oncological patients to safeguard and guarantee optimal anticancer care.
Effect of bremsstrahlung on the characteristic growth length of an avalanche of runaway electrons
Authors: Oreshkin E.
Published: July 2021

In avalanches of runaway electrons, which are formed in high-altitude atmospheric discharges, the average electron energy is several megaelectronvolts. For such high-energy electrons, the loss of energy by bremsstrahlung is significant when they collide with gas atoms. The aim of this study was to evaluate the effect of bremsstrahlung on the exponential growth length of a runaway electron avalanche. It has been shown that taking account of the bremsstrahlung produced by the electrons of an avalanche gives corrections of no more than a few percent to the avalanche exponential growth length. The corrections are maximum for electric field strengths close to the threshold below which the avalanche electrons fail to become continuously accelerated. The effect of bremsstrahlung decreases with increasing electric field strength. The higher the atomic number of the gas in which an avalanche of runaway electrons propagates, the greater the corrections.
Pneumococcal nasopharyngeal carriage and antimicrobial susceptibility profile in children under five in southern Ethiopia
Authors: Hussen Siraj , Asnake Solomon , Wachamo Demelash , Tadesse Birkneh Tilahun
Published: July 2021

Background: Streptococcus pneumonia causes high morbidity and mortality, particularly in children under five. Nasopharyngeal (NP) carriage predisposes individuals to pneumococcal infection and horizontal spread within the community. Overuse of antibiotics has been linked to increased risk of antimicrobial resistance to S. pneumonia. We investigated NP carriage rate and resistance to commonly prescribed antibiotics in under-five children visiting a public referral center in southern Ethiopia.

Methods: In total, 413 under 5 children who visited the outpatient department for a health check-up, immunization or acute mild illnesses underwent NP sampling. Parent/caregiver surveys were administered at the clinic. Sterile plastic applicator rayon tipped swabs were used for NP sampling. Antimicrobial susceptibility testing was performed using modified the disk diffusion method.

Results: S. pneumonia NP carriage was observed in 39% [95% confidence interval (CI): 34.4–43.8]. Living with one or more sibling (AOR (adjusted odds ratio) 1.95: 95% CI: 1.01, 3.76), age group of 3-23 months (AOR 2.31: 95% CI: 1.07, 4.98), co-sleeping with family (AOR 2.09, 95% CI: 1.16, 3.79), attendance at kindergarten/day-care (AOR 1.84: 95% CI: 1.09, 3.11) and malnutrition independently increased S. pneumonia carriage at the individual level. S. pneumonia was highly resistant to Oxacillin (38.5%), Tetracycline (37.3%), and Trimethoprim-sulfamethoxazole (34.2%). Multi-drug resistance was observed in 42.2% of isolates.

Conclusions: A high streptococcal NP carriage rate was observed in under-five children. The high level of resistance to commonly used antibiotics calls for enhancing national surveillance of resistance patterns and enforce antibiotic stewardship efforts.