bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and property of a novel clay anti-swelling agent
Authors: Du Weichao , Wei Jinwen , Zhang Yunyue , Chen Gang , Zhang Jie
Published: February 2020

In this work, a novel oil field clay anti-swelling agent triethylallyl ammonium bromide (TEAAB) was prepared. The effect of reaction condition on TEAAB yield was studied, and the optimal reaction conditions is reaction temperature 60 °C, reaction time 7 hours, and molar ratio of raw materials of (triethylamine: bromopropene) = 1.4. Anti-swelling results showed that TEAAB has an excellent anti-swelling performance and the anti-swelling rate is up to 98% when the dosage was 6%.
The Preparation and Characterization of Cu/AA6061 multi-layer Composte produced by Accumulated Roll Bonding
Authors: Quang P , Hao P T
Published: February 2020

The present study focuses on the preparation and characterization of Cu/AA6061 multi-layer composite materials produced by accumulated roll bonding (ARB). The bonding between metallic layers and the microstructure of samples was characterized by optical and scanning electron microscopy and tensile testing which were used to characterize the mechanical properties. After four ARB cycles, the interface only displays a little bit, showing that the good contact between layers were formed and the mechanical properties were improved. The both materials have a fine microstructure with grain sizes decreased with increasing cycles of ARB.
MIXnorm: normalizing RNA-seq data from formalin-fixed paraffin-embedded samples
Authors: Yin Shen , Wang Xinlei , Jia Gaoxiang , Xie Yang
Published: February 2020

Motivation

Recent studies have shown that RNA-sequencing (RNA-seq) can be used to measure mRNA of sufficient quality extracted from formalin-fixed paraffin-embedded (FFPE) tissues to provide whole-genome transcriptome analysis. However, little attention has been given to the normalization of FFPE RNA-seq data, a key step that adjusts for unwanted biological and technical effects that can bias the signal of interest. Existing methods, developed based on fresh-frozen or similar-type samples, may cause suboptimal performance.

Results

We proposed a new normalization method, labeled MIXnorm, for FFPE RNA-seq data. MIXnorm relies on a two-component mixture model, which models non-expressed genes by zero-inflated Poisson distributions and models expressed genes by truncated normal distributions. To obtain maximum likelihood estimates, we developed a nested EM algorithm, in which closed-form updates are available in each iteration. By eliminating the need for numerical optimization in the M-step, the algorithm is easy to implement and computationally efficient. We evaluated MIXnorm through simulations and cancer studies. MIXnorm makes a significant improvement over commonly used methods for RNA-seq expression data.

Availability and implementation

R code available at https://github.com/S-YIN/MIXnorm.

Contact

swang@smu.edu

Supplementary information

Supplementary data are available at Bioinformatics online.
Child Protection Hypothetical Case Studies for a Virtual Archive: Professional Perspectives Versus the Lived Experience and Expertise of Care Leavers in Victoria, Australia
Authors: Mendes Philip , Wilson Jacqueline Z , Golding Frank
Published: February 2020

For children in out-of-home care (OOHC) and adults who experienced OOHC as children, the records compiled by care workers, social workers and other relevant personnel present multiple ongoing problems. The records often embody deeply contested narratives that may include distortions and misinterpretations of facts, judgemental inferences, moralistic attitudes and other problematic aspects that can leave the care leaver at best ill-served and at worst profoundly distressed and traumatised. This article, an auto-ethnographic collaboration between a social work professional and two care leavers, aims to address these issues by constructing a ‘virtual archive’ consisting of several hypothetical records compiled in the style typically employed by caseworkers, which are then critiqued by the care leavers. In each case, the record is found to have significant shortcomings in terms of what is included or omitted, the tone, and implied judgements. The article concludes with a discussion that identifies a number of thematic issues and pitfalls intrinsic to the task of record-keeping in the OOHC context and makes recommendations aimed at achieving inclusive, rights-based processes and procedures in the creation and maintenance of records.
Evolution and classification of figs (Ficus, Moraceae) and their close relatives (Castilleae) united by involucral bracts
Authors: Clement Wendy L , Bruun-Lund Sam , Cohen Alanna , Kjellberg Finn , Weiblen George D , Rønsted Nina
Published: February 2020

Figs and fig wasps are a classic example of an obligate pollination mutualism. Decades of work untangling the ecology and evolution of these organisms has simultaneously contributed to development of the fields of mutualism, coevolution and plant–insect interactions at large. With > 800 species, figs (Ficus, Moraceae) are among some of the larger genera of angiosperms. Phylogenetic studies of Moraceae have supported the clade Castilleae as the sister lineage of Ficus. Compared to Ficus, Castilleae have many fewer species (60 species in 11 genera), suggesting changes in rates of diversification along these two branches. Relatively little is known about Castilleae compared to Ficus, and we argue that defining the clade comprising Ficus and Castilleae, hereafter Involucrata, focuses attention on opportunities for comparative studies of pollination mutualisms and diversification rates. In this study, we define Involucrata and propose a revised classification scheme that accounts for the phylogenetic reconstruction based on the most comprehensive sampling of this group to date. Moving forward, this classification will better guide and support evolutionary, ecological and comparative pollination biology studies of this group.
A left ventricular lead implantation at the latest site based on four-dimensional computed tomography: a case report
Authors: Matsumoto Akinori , Ogawa Ryo , Maeda Masafumi , Inakami Aya
Published: February 2020

Background

Cardiac resynchronization therapy (CRT) could be an effective therapy for patients suffering from severe heart failure (HF) despite optimal medical therapy. However, it has been reported that about 30% of patients receive ineffective results even if CRT has been performed. In a recent study, four-dimensional computed tomography (4DCT) was shown to be useful for pre-operative planning in transcatheter aortic valve intervention. The 4DCT is reconstructed with 10% increments over the cardiac cycle so that the displacement of the myocardium can be evaluated over time. From the above, we considered that the most delayed site where we would implant the left ventricular (LV) lead could be recognized by 4DCT.

Case summary

A 55-year-old man with a recurrent admission for HF indicated for CRT was referred to our hospital. In this patient, the 12-lead electrocardiogram (ECG) showed a relatively narrow QRS complex with a left bundle branch block pattern. An echocardiography demonstrated severe LV dysfunction. Although no dyssynchrony was detected, the LV lead was inserted into the most delayed site based on the 4DCT. Three-month later, the ejection fraction increased and the cardiothoracic ratio obviously shortened.

Discussion

We experienced a case in which we could evaluate the effective implantation site for the LV lead based on the 4DCT even though the effective site was not detected by echocardiography, and we could implant the LV lead at that effective site. The 4DCT may be useful for implanting LV leads in effective sites.
Platypnoea–orthodeoxia syndrome due to deformation of the patent foramen ovale caused by a dilated ascending aorta: a case report
Authors: Hasegawa Misaki , Nagai Tomoo , Murakami Tsutomu , Ikari Yuji
Published: February 2020

Background

Platypnoea–orthodeoxia syndrome (POS) is characterized by dyspnoea and arterial desaturation in the sitting position. Although its pathophysiology is complex and still needed to be investigated, the disease is one of the clinical situations which should be immediately and adequately managed by health care workers from the initial presentation.

Case summary

A 66-year-old woman with a history of systemic lupus erythematosus, deep vein thrombosis, and lumbar compression fracture was admitted for evaluation of the sudden onset of dyspnoea, while in the sitting position that was relieved on placing her in the supine position. Her transoesophageal echocardiogram did reveal a deformity in the patent foramen ovale (PFO) structure with a wide gap due to aortic compression, which was markedly different from that observed in the supine position, along with massive right-to-left shunting caused by redirected venous return due to a persistent Eustachian valve. With the computed tomography and angiograms, POS was diagnosed. Then, the patient received aortic replacement and patch closure of PFO, and her symptoms were completely resolved.

Discussion

Platypnoea–orthodeoxia syndrome is a condition with quite unique features and needs multiple clinical measures for the diagnosis and medical management. For all health care workers, it is essential to have a high suspicion in order to detect POS in patients with unexplained dyspnoea. Echocardiography plays a major role in establishing the diagnosis and offering the choice of therapeutic options.
Impact of frailty on outcomes in elderly patients with acute myocardial infarction who undergo percutaneous coronary intervention
Authors: Nishihira Kensaku , Yoshioka Goro , Kuriyama Nehiro , Ogata Kenji , Kimura Toshiyuki , Matsuura Hirohide , Furugen Makoto , Koiwaya Hiroshi , Watanabe Nozomi , Shibata Yoshisato
Published: February 2020

Aims 

Frailty is characterized by reduced biological reserves and weakened resistance to stressors, and is common in older adults. This study evaluated the prognostic implications of frailty at hospitalization in elderly patients with acute myocardial infarction (AMI) who undergo percutaneous coronary intervention (PCI).

Methods and results 

We prospectively analysed 546 AMI patients aged ≥80 years undergoing PCI from 2009 to 2017. Frailty was classified based on impairment in walking (unassisted, assisted, and wheelchair/non-ambulatory), cognition (normal, mildly impaired, moderately to severely impaired), and basic activities of daily living. Impairment in each domain was scored as 0, 1, or 2, and patients were categorized into the following three groups based on total score: no frailty (0), mild frailty (1–2), moderate-to-severe frailty (≥3). The median follow-up period was 589 days. Of the 546 patients, 27.8% were frail (mild or moderate-to-severe), and this proportion significantly increased to 35.5% at discharge (P < 0.001). Compared to non-frail patients, frail patients were older, less likely to be male, and had a higher rate of advanced Killip class. Major bleeding (no frailty, 9.6%; mild frailty, 16.9%; moderate-to-severe frailty, 31.8%; P < 0.001) and in-hospital mortality (no frailty, 8.4%; mild frailty, 15.4%; moderate-to-severe frailty, 27.3%; P < 0.001) increased as frailty worsened. After adjusting for confounders, frailty was independently associated with higher mid-term all-cause mortality (hazard ratio, 1.81; 95% confidence interval, 1.23–2.65; P = 0.002).

Conclusion 

Frailty in AMI patients aged ≥80 years undergoing PCI was associated with major bleeding, in-hospital death, and mid-term mortality.
High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to-macrophage transition
Authors: Hinneburg Hannes , Pedersen Jessica L , Bokil Nilesh J , Pralow Alexander , Schirmeister Falko , Kawahara Rebeca , Rapp Erdmann , Saunders Bernadette M , Thaysen-Andersen Morten
Published: February 2020

Protein glycosylation impacts the development and function of innate immune cells. The glycophenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described. Herein, label-free porous graphitised carbon–liquid chromatography–tandem mass spectrometry (PGC-LC-MS/MS) was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition. Primary blood-derived CD14+ monocytes were differentiated ex vivo in the absence of strong anti- and proinflammatory stimuli using a conventional 7-day granulocyte-macrophage colony-stimulating factor differentiation protocol with longitudinal sampling. Morphology and protein expression monitored by light microscopy and proteomics validated the maturation process. Glycomics demonstrated that monocytes and macrophages display similar N-glycome profiles, comprising predominantly paucimannosidic (Man1-3GlcNAc2Fuc0–1, 22.1–30.8%), oligomannosidic (Man5-9GlcNAc2, 29.8–35.7%) and α2,3/6-sialylated complex-type N-glycans with variable core fucosylation (27.6–39.1%). Glycopeptide analysis validated conjugation of these glycans to human proteins, while quantitative proteomics monitored the glycoenzyme expression levels during macrophage differentiation. Significant interperson glycome variations were observed suggesting a considerable physiology-dependent or heritable heterogeneity of CD14+ monocytes. Only few N-glycome changes correlated with the monocyte-to-macrophage transition across donors including decreased core fucosylation and reduced expression of mannose-terminating (paucimannosidic-/oligomannosidic-type) N-glycans in macrophages, while lectin flow cytometry indicated that more dramatic cell surface glycan remodelling occurs during maturation. The less heterogeneous core 1-rich O-glycome showed a minor decrease in core 2-type O-glycosylation but otherwise remained unchanged with macrophage maturation. This high-resolution glycome map underpinning normal monocyte-to-macrophage transition, the most detailed to date, aids our understanding of the molecular makeup pertaining to two vital innate immune cell types and forms an important reference for future glycoimmunological studies.
A novel homozygous mutation of phospholipase C zeta leading to defective human oocyte activation and fertilization failure
Authors: Yuan Peng , Yang Cen , Ren Yixin , Yan Jie , Nie Yanli , Yan Liying , Qiao Jie
Published: February 2020

STUDY QUESTION

Is a novel homozygous phospholipase C zeta (PLCζ), c.1658 G>C; p. R553P mutation in the C2 domain associated with the outcomes of recurrent fertilization failure after ICSI?

SUMMARY ANSWER

PLCζ, c.1658 G>C led to defective human oocyte activation and fertilization failure, while this mutation in the C2 domain of PLCζ did not compromise concentration, motility and chromosome ploidy of sperm.

WHAT IS KNOWN ALREADY

Sperm-specific PLCζ is now widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations, which are essential for egg activation during mammalian fertilization. Thus far, few genetic studies have shown that different point mutations in the PLCζ gene are associated with male infertility.

STUDY DESIGN, SIZE, DURATION

This was a basic medical research to assess pathogenicity for novel mutation in the C2 domain of PLCζ during human fertilization.

PARTICIPANTS/MATERIALS, SETTING, METHODS

Single-cell omics were applied to analyze the DNA methylation state of the fertilization failure oocytes and the ploidy of the patient’s sperm. Whole genome sequencing data for the patient were analyzed for mutations in PLCζ. Sanger sequencing confirmed the presence of a rare variant, and then the mutant and wild-type PLCζ mRNA were injected to observe oocyte activation.

MAIN RESULTS AND THE ROLE OF CHANCE

The fertilization failure oocytes (n = 4) were triploid and lacking proper DNA demethylation. The whole genome sequencing analysis revealed a novel missense homozygous mutation in PLCζ, c.1658 G>C; p. R553P, which leads to the conversion of arginine 553 to proline. This point mutation does not affect the production of the corresponding protein in sperm. However, microinjection of the mRNA transcribed from the PLCζ R553P mutation gene failed to trigger oocyte activation and the subsequent embryo development.

LIMITATIONS, REASONS FOR CAUTION

Only one patient with PLCζ mutations was available because of its rare incidence.

WIDER IMPLICATIONS OF THE FINDINGS

Notably, we discovered a novel homozygous mutation in PLCζ, which results in an abnormal conformation at the C2 domain of the PLCζ protein. Our findings indicate an essential role of PLCζ in human fertilization and the requirement of a normal structure of C2 domain in PLCζ-mediated physiological function.

STUDY FUNDING/COMPETING INTEREST(S)

This project is funded by the National Natural Science Foundation of China (31571544, 31871482, 31871447) and National Key Research and Development Program (2018YFC1004000, 2017YFA0103801). All authors declared no competing interests.

TRIAL REGISTRATION NUMBER

Not applicable.
Repositioning rafoxanide to treat Gram-negative bacilli infections
Authors: Miró-Canturri Andrea , Ayerbe-Algaba Rafael , Villodres Ángel Rodríguez , Pachón Jerónimo , Smani Younes
Published: February 2020

Objectives

Repurposing drugs provides a new approach to the fight against MDR Gram-negative bacilli (MDR-GNB). Rafoxanide, a veterinary antihelminthic drug, has shown antibacterial activity in vitro against Gram-positive bacteria. We aimed to analyse the in vitro and in vivo efficacy of rafoxanide in combination with colistin against colistin-susceptible (Col-S) and colistin-resistant (Col-R) GNB.

Methods

A collection of Col-S and Col-R Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae were used. Chequerboard and time–kill curve analyses were performed to determine the synergy between rafoxanide and colistin. Changes in membrane structure and permeability were analysed using transmission electron microscopy and fluorescence assays. A murine peritoneal sepsis model using Col-R strains of these pathogens was performed to study the efficacy of rafoxanide (10 mg/kg/24 h, IV), colistimethate sodium (CMS) (20 mg/kg/8 h, intraperitoneally) and rafoxanide (10 mg/kg/24 h, IV) plus CMS (20 mg/kg/8 h, intraperitoneally) for 72 h.

Results

Rafoxanide showed MICs ≥256 mg/L for all Col-S and Col-R strains. Chequerboard and time–kill curve analyses showed that rafoxanide (1 mg/L) is more synergistic with colistin against Col-R than Col-S strains. Col-R, but not Col-S, strains treated with rafoxanide demonstrated higher membrane permeabilization. Transmission electron microscopy visualization confirmed that Col-R strains suffer morphological changes. In the murine peritoneal sepsis model with Col-R strains, rafoxanide plus CMS, compared with CMS alone, increased mouse survival to 53.8% and 73.3%, and reduced bacterial loads in tissues and blood between 2.34 and 4.99 log10 cfu/g or mL, respectively.

Conclusions

Rafoxanide repurposing, as monotherapy and in combination with CMS, may address the urgent need for new treatments for infections caused by MDR-GNB.
Comparison of Two Commercially Available Fentanyl Screening Immunoassays for Clinical Use
Authors: Budelier Melissa M , Franks Caroline E , Logsdon Nicole , Jannetto Paul J , Scott Mitchell G , Roper Stephen M , Farnsworth Christopher W
Published: February 2020

Background

Fentanyl is a synthetic opioid associated with illicit drug use and overdose deaths. The SEFRIA Immunalysis (IAL) and ARK fentanyl assays are both FDA-cleared, open channel immunoassays for fentanyl detection in urine. However, limited data are available in the literature comparing these assays. The objective of this study was to perform a direct comparison of these two fentanyl immunoassays.

Methods

IAL and ARK fentanyl immunoassays were performed on a Roche Cobas e602 automated chemistry analyzer. Repeatability and total imprecision were compared by diluting fentanyl into urine at concentrations above, below, and at the manufacturers’ cutoffs of 1.0 ng/mL. Cross-reactivity was assessed for norfentanyl and the fentanyl analogs acetylfentanyl, acrylfentanyl, and furanylfentanyl. Concordance was assessed in 90 patient samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) as the gold standard.

Results

Repeatability varied from 11.4%–17.8% on the IAL assay and 2.8%–5.5% on the ARK assay. Total imprecision was 18.9%–40.7% on the IAL assay and 2.9%–6.4% on the ARK assay. Both assays cross-reacted with acetylfentanyl (∼100%), acrylfentanyl (∼100%), and furanylfentanyl (∼20%), but only the ARK assay cross-reacted with norfentanyl (∼3%). An admixture of 0.5 ng/mL fentanyl and 6 ng/mL norfentanyl produced a positive result on the ARK assay. Total concordance between IAL and ARK for 90 tested patient samples was 93% (kappa = 0.85). Relative to LC-MS/MS, the IAL assay had a concordance of 90% (kappa = 0.79) and the ARK assay had a concordance of 94% (kappa = 0.88). Including norfentanyl in the LC-MS/MS confirmation increased the concordance of the ARK to 96% (kappa = 0.90).

Conclusions

The ARK assay recognized the metabolite norfentanyl, demonstrated superior precision, and had better concordance with LC-MS/MS compared to the IAL assay.
How accurate is the medical record? A comparison of the physician’s note with a concealed audio recording in unannounced standardized patient encounters
Authors: Weiner Saul J , Wang Shiyuan , Kelly Brendan , Sharma Gunjan , Schwartz Alan
Published: February 2020

Objectives

Accurate documentation in the medical record is essential for quality care; extensive documentation is required for reimbursement. At times, these 2 imperatives conflict. We explored the concordance of information documented in the medical record with a gold standard measure.

Materials and Methods

We compared 105 encounter notes to audio recordings covertly collected by unannounced standardized patients from 36 physicians, to identify discrepancies and estimate the reimbursement implications of billing the visit based on the note vs the care actually delivered.

Results

There were 636 documentation errors, including 181 charted findings that did not take place, and 455 findings that were not charted. Ninety percent of notes contained at least 1 error. In 21 instances, the note justified a higher billing level than the gold standard audio recording, and in 4, it underrepresented the level of service (P = .005), resulting in 40 level 4 notes instead of the 23 justified based on the audio, a 74% inflated misrepresentation.

Discussion

While one cannot generalize about specific error rates based on a relatively small sample of physicians exclusively within the Department of Veterans Affairs Health System, the magnitude of the findings raise fundamental concerns about the integrity of the current medical record documentation process as an actual representation of care, with implications for determining both quality and resource utilization.

Conclusion

The medical record should not be assumed to reflect care delivered. Furthermore, errors of commission—documentation of services not actually provided—may inflate estimates of resource utilization.
TRIO Method for Detection of Beta-Lactams, Sulfonamides, and Tetracyclines in Raw Commingled Cows' Milk
Authors: Salter Robert S , Markovsky R J , Douglas D W , Saul S J , Tran A C , Legg D R , Schwartz J A , Conaway D M , McRobbie L W , Kalinowski Emily , Bulthaus Mary
Published: February 2020

A qualitative 3 min one-step assay for detecting beta-lactam, sulfonamide, and tetracycline antibiotics was validated following milk screening test guidelines developed by FDA-CVM, AOAC-RI, and IDF. The validated 90% detection levels with 95% confidence were: penicillin G 2 part per billion (ppb); amoxicillin 4 ppb; ampicillin 9 ppb; ceftiofur plus metabolites 50 ppb; cloxacillin 9 ppb; cephapirin 15 ppb; sulfadimethoxine 8 ppb; sulfamethazine 9 ppb; chlortetracycline 34 ppb; oxytetracycline 53 ppb; and tetracycline 42 ppb. Detection levels were lower than U.S. and Canadian allowable limits for milk and were consistent with most European Maximum Residue Limits. Tests of raw commingled cows’ milk indicated a low positive error rate of <0.3% with no interferences demonstrated by 1.08 MM/mL somatic cells, Gram-positive or Gram-negative bacteria < 300 K/mL, freeze/thawing, or non-targeted drugs. Detection of incurred residues were similar to, or more sensitive to, fortified samples. Some cross reactivity across drug families occurred in interference studies and therefore initial positive samples should be confirmed with drug family specific screening methods. The National Conference of Interstate Milk Shipments approval as a bulk tank/tanker screening test was completed in three stages for each drug family, including a tetracycline confirmation procedure to target U.S. tolerance levels. Detection and robustness were found to be appropriate for multiple countries’ regulatory requirements for screening tests. The method development, validation, and approval was intended to diversify and increase the verification tools for the control of the major antibiotic drug families used in managing cows’ health and welfare.
Closure of Persistent Tracheocutaneous Fistulas in Pediatric Burn Patients*
Authors: Camargo Lauren , Heiman Adee J , Ricci Joseph A
Published: February 2020

Tracheocutaneous fistula (TCF) is a common complication that occurs after decannulation of a long-term tracheostomy. Numerous studies have demonstrated the incidence of TCF formation to positively correlate with an increasing duration of cannulation, specifically in children. Treatment of a persistent TCF in a child has been well described in the literature, with good response to local measures such as curettage and silver nitrate. When this fails, fistulectomy followed by primary closure of the skin or secondary intention yields good results. However, there is a lack of knowledge on TCF formation in pediatric burn-injured patients, where a persistent TCF is a particularly challenging problem to correct given the paucity of supple tissue in the neck and potential for contractures after a large burn injury; effectively making the surgical repairs and management algorithms described in the available literature largely not applicable to this patient population. In this manuscript, we describe a series of pediatric burn patients with persistent TCF, successfully treated with a multilayered closure involving local tissue rearrangement in the form of medial mobilization of the strap muscles of the neck.
Birds of a Feather Feel Together: Emotional Ability Similarity in Consumer Interactions
Authors: Kidwell Blair , Lopez-Kidwell Virginie , Blocker Christopher , Mas Erick M
Published: February 2020

The authors introduce emotional ability similarity to explain consumer satisfaction in interactions with frontline sales and service employees and other consumers beyond the effects of traditional relational variables in the similarity–attraction paradigm. Four studies examine how and why similar abilities for using emotional information between two people promote relational success in marketplace exchanges. We find that, when interacting with others, consumers who exchange nonverbal information with their partners experience (dis)similarity in their emotional ability (EA). Similar dyads who rely on expressive (high–high EA pairs) or inexpressive (low–low EA pairs) emotion norms experience significantly greater satisfaction in their interactions than consumers with dissimilar norms (high–low EA pairs). Together, these findings advance the understanding of consumer relationships and satisfaction by establishing EA similarity as a new avenue for consumer research.
Long-term outcomes of trans-oral robotic surgery-assisted total laryngectomy for recurrent laryngeal cancers
Authors: Wang Chen-Chi , Lin Wen-Jiun , De Virgilio Armando , Liu Shih-An , Chen Sheng-Hwa , Liao Jia-Shiou
Published: February 2020

Objective

For recurrent laryngeal cancers, trans-oral robotic surgery has been used to perform total laryngectomy, but limited cases had been reported without long-term outcome follow-up. This study aims at presenting the largest longitudinal retrospective cohort in a single tertiary referral medical center.

Methods

From November 2013 to August 2017, seven patients with recurrent laryngeal cancers without evidence of neck metastasis were selected to receive trans-oral robotic surgery-assisted TL without neck dissection. The para-operative details including the surgical success rates, surgical methods, resection extent, drainage tube placement, pharyngeal wound closure, console surgical time, pathologic findings and long-term complications were reviewed and described. The study end points include survival rates and preservation of swallowing function without tube feeding.

Results

Trans-oral robotic surgery-assisted total laryngectomy was successfully performed on all seven patients with mean surgical console time of 111 ± 66 min. Strap muscles and hyoid bone were resected like open surgery in six and five patients, respectively. For all the seven patients, there was no severe pharyngo-cutaneous fistula formation requiring repair in a second surgery, but tracheostoma stenosis was not uncommon (57%). Three patients received adjuvant chemotherapy/radiotherapy. After follow-up of 36.1 ± 15.8 months, two patients had neck recurrence, and one patient died 19 months after surgery, but the other five patients were alive without disease recurrence. The overall survival rate was 85.7% (6/7), and all patients had good swallowing function without tube feeding.

Conclusions

Trans-oral robotic surgery-assisted total laryngectomy is a feasible approach for selected patients with recurrent laryngeal cancers. The oncologic and functional outcomes were satisfactory. Further larger cohort study is worthwhile to further elucidate the value of trans-oral robotic surgery-assisted total laryngectomy.
Potential Roles of Environmental and Socio-Economic Factors in the Distribution of Insecticide Resistance in Anopheles gambiae sensu lato (Culicidae: Diptera) Across Togo, West Africa
Authors: Ahadji-Dabla Koffi Mensah , Romero-Alvarez Daniel , Djègbè Innocent , Amoudji Adjovi Djifa , Apétogbo Georges Yawo , Djouaka Rousseau , Oboussoumi Komlanvi , Aawi Agnidoufèyi , Atcha-Oubou Tinah , Peterson A Townsend , Ketoh Guillaume Koffivi
Published: February 2020

Vector control strategies recommended by the World Health Organization are threatened by resistance of Anopheles mosquitoes to insecticides. Information on the distribution of resistant genotypes of malaria vectors is increasingly needed to address the problem. Ten years of published and unpublished data on malaria vector susceptibility/resistance and resistance genes have been collected across Togo. Relationships between the spatial distribution of resistance status and environmental, socio-economic, and landscape features were tested using randomization tests, and calculating Spearman rank and Pearson correlation coefficients between mosquito mortality and different gridded values. Anopheles gambiae sensu lato was resistant to DDT, pyrethroids, and the majority of carbamates and organophosphates. Three sibling species were found (i.e., An. gambiae, Anopheles coluzzii, and Anopheles arabiensis) with four resistance genes, including kdr (L1014F, L1014S, and N1575Y) and ace1 (G119S). The most frequent resistance gene was L1014F. Overall, no association was found between the susceptibility/resistance status and environmental features, suggesting that evolution of resistance may be most closely related to extreme selection from local insecticide use. Nevertheless, further research is necessary for firm conclusions about this lack of association, and the potential role of landscape characteristics such as presence of crops and percentage of tree cover.
Do We Need Rights in Bioethics Discourse?
Authors: Sim Julius
Published: February 2020

Moral rights feature prominently and are relied on substantially in debates in bioethics. Conceptually, however, duties can perform the logical work of rights, but not vice versa, and reference to rights is therefore inessential. Normatively, rights, like duties, depend on more basic moral values or principles, and attempts to establish the logical priority of rights over duties or the reverse are misguided. In practical decision making, however, an analysis in terms of duties is more fruitful than one based on rights. A right may function as a proxy term for a consequentialist rule, or for a deontological constraint, but does not thereby enrich these concepts. Rights may also help in a purely expressive sense and may assist an initial focusing on a moral conflict. However, their role in bioethics discourse is more one of convenience than of necessity. Moreover, unless rights are firmly founded on fundamental moral values, their use encourages rhetoric rather than argument.
Music Therapy for Preterm Infants and Their Parents: A Cluster-Randomized Controlled Trial Protocol
Authors: Yakobson Dana , Arnon Shmuel , Gold Christian , Elefant Cochavit , Litmanovitz Ita , Beck Bolette Daniels
Published: February 2020

Music therapy (MT) interventions and skin-to-skin care (SSC) both aim to address the varied needs of preterm infants, including sensory regulation and stress reduction, inclusion of parents in their infant’s care, support of parents’ emotional state, and enhancing the parent–infant attachment process. Few studies have investigated the combination of both modalities through randomized controlled trials. Evidence of longer-term effects is missing. This article presents a study protocol that will investigate the effects of combined family-centered MT intervention and SSC on preterm-infants’ autonomic nervous system (ANS) stability, parental anxiety levels, and parent–infant attachment quality. 12 clusters with a total of 72 preterm infants, with their parents, will be randomized to one of two conditions: MT combined with SSC or SSC alone. Each parent–infant dyad will participate in 3 sessions (2 in the hospital and a 3-month follow-up). The primary outcome of preterm infants’ ANS stability will be measured by the high frequency power of their heart rate variability. Secondary outcomes will be physiological measures and behavioral states in infants and anxiety and attachment levels of parents. This trial will provide important, evidence-based knowledge on the use of the “First Sounds: Rhythm, Breath, and Lullaby” model of MT in neonatal care, through an intervention that is in line with the Newborn Individualized Developmental Care and Assessment Program model for supportive developmental care of preterm infants and their parents. Ethical approval (no. 0283-15) was granted from the local Institutional Review Board in April 2017. This trial is registered in ClinicalTrials.gov, NCT03023267.
Pelvic angiomyofibroblastoma: an unusual case report
Authors: Laraqui Housseini Yasmine , Elouazzani Hafsa , Housseini Laila Laraqui , Cherkaoui Meriem , Zouaidia Fouad
Published: February 2020

Angiomyofibroblastoma is a rare mesenchymal benign tumor that frequently occurs in young- to middle-aged women, arising from the genital tract. There are many overlapping radiological and immunohistochemical features with other stromal cell lesions, making the diagnosis difficult. We report here a case of a 29-year-old woman admitted for a pelvic mass, in whom, the histopathological and immunohistochemical studies led to the diagnosis of angiomyofibroblastoma.
¿Existe correlación entre importancia y centralidad? Evaluación de personajes con redes sociales en obras teatrales de la Edad de Plata
Authors: Santa María María Teresa , Calvo Tello José , Jiménez Concepción María
Published: February 2020

The objective of this study is to answer the question: Do the central nodes of a social network of characters correspond with the protagonists of a theater play? To answer this question we evaluate different measures of centrality along with other textual quantitative values in relation to other manually annotated metadata on a corpus of twenty five dramatic plays of the Spanish theatre of the Silver Age (1868-1936). The found results show that centrality correlates moderately with importance, but the correlation with the textual quantitative values is stronger.
Probing the temperature structure of optically thick discs using polarized emission of aligned grains
Authors: Lin Zhe-Yu Daniel , Li Zhi-Yun , Yang Haifeng , Looney Leslie , Lee Chin-Fei , Stephens Ian , Lai Shih-Ping
Published: February 2020

Polarized continuum emission from aligned grains in discs around young stellar objects can be used to probe the magnetic field, radiation anisotropy, or drift between dust and gas, depending on whether the non-spherical grains are aligned magnetically, radiatively, or mechanically. We show that it can also be used to probe another key disc property – the temperature gradient – along sightlines that are optically thick, independent of the grain alignment mechanism. We first illustrate the technique analytically using a simple 1D slab model, which yields an approximate formula that relates the polarization fraction to the temperature gradient with respect to the optical depth τ at the τ = 1 surface. The formula is then validated using models of stellar irradiated discs with and without accretion heating. The promises and challenges of the technique are illustrated with a number of Class 0 and I discs with ALMA dust polarization data, including NGC 1333 IRAS4A1, IRAS 16293B, BHB 07-11, L1527, HH 212, and HH 111. We find, in particular, that the sightlines passing through the near-side of a highly inclined disc trace different temperature gradient directions than those through the far-side, which can lead to a polarization orientation on the near-side that is orthogonal to that on the far-side, and that the HH 111 disc may be such a case. Our technique for probing the disc temperature gradient through dust polarization can complement other methods, particularly those using molecular lines.
A possible distance bias for type Ia supernovae with different ejecta velocities
Authors: Siebert M R , Foley R J , Jones D O , Davis K W
Published: February 2020

After correcting for their light-curve shape and colour, Type Ia supernovae (SNe Ia) are precise cosmological distance indicators. However, there remains a non-zero intrinsic scatter in the differences between measured distance and that inferred from a cosmological model (i.e. Hubble residuals or HRs), indicating that SN Ia distances can potentially be further improved. We use the open-source relational data base kaepora to generate composite spectra with desired average properties of phase, light-curve shape, and HR. At many phases, the composite spectra from two subsamples with positive and negative average HRs are significantly different. In particular, in all spectra from 9 d before to 15 d after peak brightness, we find that SNe with negative HRs have, on average, higher ejecta velocities (as seen in nearly every optical spectral feature) than SNe with positive HRs. At +4 d relative to B-band maximum, using a sample of 62 SNe Ia, we measure a 0.091 ± 0.035 mag (2.7σ) HR step between SNe with Si ii λ6355 line velocities ($v_{Si\, rm{\small II}}$) higher/lower than −11 000 km s−1 (the median velocity). After light-curve shape and colour correction, SNe with higher velocities tend to have underestimated distance moduli relative to a cosmological model. The intrinsic scatter in our sample reduces from 0.094 to 0.082 mag after making this correction. Using the Si ii λ6355 velocity evolution of 115 SNe Ia, we estimate that a velocity difference >500 km s−1 exists at each epoch between the positive-HR and negative-HR samples with 99.4 per cent confidence. Finally at epochs later than +37 d, we observe that negative-HR composite spectra tend to have weaker spectral features in comparison to positive-HR composite spectra.
The self-similar structure of advection-dominated discs with outflow and radial viscosity
Authors: Ghoreyshi S M , Shadmehri M
Published: February 2020

Observational evidence and theoretical arguments postulate that outflows may play a significant role in the advection-dominated accretion discs (ADAFs). While the azimuthal viscosity is the main focus of most previous studies in this context, recent studies indicated that disc structure can also be affected by the radial viscosity. In this work, we incorporate these physical ingredients and the toroidal component of the magnetic field to explore their roles in the steady-state structure of ADAFs. We thereby present a set of similarity solutions where outflows contribute to the mass loss, angular momentum removal, and the energy extraction. Our solutions indicate that the radial viscosity causes the disc to rotate with a slower rate, whereas the radial gas velocity increases. For strong winds, the infall velocity may be of order the Keplerian speed if the radial viscosity is considered and the saturated conduction parameter is high enough. We show that the strength of magnetic field and of wind can affect the effectiveness of radial viscosity.
Mass transfer of low-mass binaries and chemical anomalies among unevolved stars in globular clusters
Authors: Wei Dandan , Wang Bo , Chen Xuefei , Chen Hailiang , Li Lifang , Jiang Dengkai
Published: February 2020

While it is well known that mass transfer in binaries can pollute the surfaces of the accretors, it is still unclear whether this mechanism can reproduce the observed chemical inhomogeneities in globular clusters. We study the surface abundances of the accretors in low-mass binaries, as a first step towards understanding whether mass transfer in low-mass binaries is one of the potential origins of the aforementioned abundance anomalies in globular clusters. We use the mesa (Modules for Experiments in Stellar Astrophysics) code to calculate binary evolutionary models with different initial donor masses between 0.9 and 1.9 $\rm {M}_\odot$ for an initial metallicity of Z = 0.0034. The results show that in some low-mass binary systems, the accretors exhibit peculiar chemical patterns when they are still unevolved stars, e.g. C and O depletion; Na and N enhancement; and constant Mg, Al, and C+N+O. The abundance patterns of the accretors are significantly different from their initial abundances (or that of normal single stars), and can match the observed populations. These abundance patterns strongly depend not only on the initial parameters of binaries (donor mass, mass ratio, and orbital period), but also on the assumptions regarding mass-transfer efficiency and angular momentum loss. These results support the hypothesis that mass transfer in low-mass binaries is, at least, partly responsible for the unevolved anomalous stars in globular clusters. More work on binary evolutionary models and binary population synthesis is required to fully evaluate the contribution of this scenario.
H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles
Authors: Belotti Edwige , Lacoste Nicolas , Simonet Thomas , Papin Christophe , Padmanabhan Kiran , Scionti Isabella , Gangloff Yann-Gaël , Ramos Lorrie , Dalkara Defne , Hamiche Ali , Dimitrov Stefan , Schaeffer Laurent
Published: February 2020

While the histone variant H2A.Z is known to be required for mitosis, it is also enriched in nucleosomes surrounding the transcription start site of active promoters, implicating H2A.Z in transcription. However, evidence obtained so far mainly rely on correlational data generated in actively dividing cells. We have exploited a paradigm in which transcription is uncoupled from the cell cycle by developing an in vivo system to inactivate H2A.Z in terminally differentiated post-mitotic muscle cells. ChIP-seq, RNA-seq and ATAC-seq experiments performed on H2A.Z KO post-mitotic muscle cells show that this histone variant is neither required to maintain nor to activate transcription. Altogether, this study provides in vivo evidence that in the absence of mitosis H2A.Z is dispensable for transcription and that the enrichment of H2A.Z on active promoters is a marker but not an active driver of transcription.
A novel transcriptional cascade is involved in Fzr-mediated endoreplication
Authors: Qian Wenliang , Li Zheng , Song Wei , Zhao Tujing , Wang Weina , Peng Jian , Wei Ling , Xia Qingyou , Cheng Daojun
Published: February 2020

Endoreplication, known as endocycle, is a variant of the cell cycle that differs from mitosis and occurs in specific tissues of different organisms. Endoreplicating cells generally undergo multiple rounds of genome replication without chromosome segregation. Previous studies demonstrated that Drosophila fizzy-related protein (Fzr) and its mammalian homolog Cdh1 function as key regulators of endoreplication entrance by activating the anaphase-promoting complex/cyclosome to initiate the ubiquitination and subsequent degradation of cell cycle factors such as Cyclin B (CycB). However, the molecular mechanism underlying Fzr-mediated endoreplication is not completely understood. In this study, we demonstrated that the transcription factor Myc acts downstream of Fzr during endoreplication in Drosophila salivary gland. Mechanistically, Fzr interacts with chromatin-associated histone H2B to enhance H2B ubiquitination in the Myc promoter and promotes Myc transcription. In addition to negatively regulating CycB transcription, the Fzr-ubiquitinated H2B (H2Bub)-Myc signaling cascade also positively regulates the transcription of the MCM6 gene that is involved in DNA replication by directly binding to specific motifs within their promoters. We further found that the Fzr-H2Bub-Myc signaling cascade regulating endoreplication progression is conserved between insects and mammalian cells. Altogether, our work uncovers a novel transcriptional cascade that is involved in Fzr-mediated endoreplication.
A posteriori dietary patterns and their association with systemic low-grade inflammation in adults: a systematic review and meta-analysis
Authors: Norde Marina M , Collese Tatiana S , Giovannucci Edward , Rogero Marcelo M
Published: February 2020

Context

A posteriori dietary patterns are promising ways of uncovering potential public health strategies for the prevention of systemic, low-grade, inflammation-related, chronic noncommunicable diseases.

Objective

To investigate and summarize the current evidence on the association between a posteriori dietary patterns and systemic, low-grade inflammation in adults.

Data sources

MEDLINE, EMBASE, Web of Science, and LILACS were searched.

Data extraction

Data screening, extraction, and quality assessment were performed independently by 2 investigators. Meta-analysis with random effects was conducted. Differences and similarities between reduced rank regression–derived dietary patterns were assessed.

Results

Healthy dietary patterns are inversely and the Western dietary pattern is positively associated with inflammation (r = −0.13, 95% confidence interval −0.20 to −0.06; and r = 0.11, 95% confidence interval, 0.09–0.12, respectively). Reduced rank regression–derived anti-inflammatory dietary patterns are consistently characterized by high intake of fresh fruits and inflammatory dietary patterns are consistently characterized by high intake of red and processed meat and low intake of vegetables.

Conclusion

Favoring the substitution of a Westernized diet for a healthy diet may lower inflammation, which might improve the prevention of some chronic noncommunicable diseases.