bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Modified-Intake Plasma-Enhanced Metal–Organic Chemical Vapor Deposition System to Grow Gallium Doped Zinc Oxide
Authors: Lei Po-Hsun , Chen Jia-Jan , Song Ming-Hsiu , Zhan Yuan-Yu , Jiang Zong-Lin
Published: December 2021

We have used a modified-intake plasma-enhanced metal–organic chemical vapor deposition (MIPEMOCVD) system to fabricate gallium-doped zinc oxide (GZO) thin films with varied Ga content. The MIPEMOCVD system contains a modified intake system of a mixed tank and a spraying terminal to deliver the metal–organic (MO) precursors and a radio-frequency (RF) system parallel to the substrate normal, which can achieve a uniform distribution of organic precursors in the reaction chamber and reduce the bombardment damage. We examined the substitute and interstitial mechanisms of Ga atoms in zinc oxide (ZnO) matrix in MIPEMOCVD-grown GZO thin films through crystalline analyses and Hall measurements. The optimal Ga content of MIPEMOCVD-grown GZO thin film is 3.01 at%, which shows the highest conductivity and transmittance. Finally, the optimal MIPEMOCVD-grown GZO thin film was applied to n-ZnO/p-GaN LED as a window layer. As compared with the indium–tin–oxide (ITO) window layer, the n-ZnO/p-GaN LED with the MIPEMOCVD-grown GZO window layer of the rougher surface and higher transmittance at near UV range exhibits an enhanced light output power owing to the improved light extraction efficiency (LEE).
Proteomics Readjustment of the Yarrowia lipolytica Yeast in Response to Increased Temperature and Alkaline Stress
Authors: Sekova Varvara Y. , Kovalyov Leonid I. , Kovalyova Marina A. , Gessler Natalya N. , Danilova Maria A. , Isakova Elena P. , Deryabina Yulia I.
Published: December 2021

Yeasts cope with a wide range of environmental challenges using different adaptive mechanisms. They can prosper at extreme ambient pH and high temperatures; however, their adaptation mechanisms have not been entirely investigated. Previously, we showed the pivotal role and flexibility of the sugar and lipid composition of Yarrowia lipolytica W 29 upon adaptation to unfavorable conditions. In this study, we showed that extreme pH provoked significant changes in the cell wall proteins expression, with an increase in both the chaperones of heat shock protein HSP60 and some other proteins with chaperone functions. The mitochondria activity changes inducing the VDAC and malate dehydrogenase played an essential role in the adaptation, as did the altered carbohydrate metabolism, promoting its shift towards the pyruvate formation rather than gluconeogenesis. The elevated temperature led to changes in the cell wall proteins and chaperones, the induced expression of the proteins involved in the cell structural organization, ribosomal proteins, and the enzymes of formaldehyde degradation. Moreover, the readjustment of the protein composition and amount under combined stress indicated the promotion of catabolic processes related to scavenging the damaged proteins and lipids. Under all of the stress conditions studied, the process of folding, stress resistance, redox adaptation, and oxidative phosphorylation were the dominant pathways. The combined chronic alkaline and heat stress (pH 9.0, 38 °C) led to cross-adaptation, which caused “switching” over the traditional metabolism to the adaptation to the most damaging stress factor, namely the increased temperature.
Immune Response to BNT162b2 in Solid Organ Transplant Recipients: Negative Impact of Mycophenolate and High Responsiveness of SARS-CoV-2 Recovered Subjects against Delta Variant
Authors: Cassaniti Irene , Bergami Federica , Arena Francesca , Sammartino Jose Camilla , Ferrari Alessandro , Zavaglio Federica , Curti Irene , Percivalle Elena , Meloni Federica , Pandolfi Laura , Pellegrini Carlo , Turco Annalisa , Seminari Elena , Pattonieri Eleonora Francesca , Gregorini Marilena , Rampino Teresa , Sarasini Antonella , Lilleri Daniele , Baldanti Fausto
Published: December 2021

The immunogenicity of severe acute respiratory syndrome 2 virus (SARS-CoV-2) vaccines in immunocompromised patients remains to be further explored. Here, we evaluated the immunogenicity elicited by complete vaccination with BNT162b2 vaccine in solid organ transplant recipients (SOTRs). A cohort of 110 SOTRs from Northern Italy were vaccinated with two doses of BNT162b2 mRNA vaccine and prospectively monitored at baseline and after 42 days. Both SARS-CoV-2 naïve and recovered subjects were included. Humoral response elicited by vaccination, including SARS-CoV-2 neutralizing antibodies (SARS-CoV-2 NT Abs), was evaluated; additionally, ex-vivo ELISpot assay was performed for the quantification of Spike-specific T-cell response. Results were compared with those obtained in a cohort of healthy subjects. In a subset of patients, humoral and T-cell responses against delta variant were also evaluated. Less than 20% of transplanted subjects developed a positive humoral and cell-mediated response after complete vaccination schedule. Overall, median levels of immune response elicited by vaccination were significantly lower with respect to controls in SARS-CoV-2 naïve transplant, but not in SARS-CoV-2 recovered transplanted patients. Additionally, a significant impairment of both humoral and cell-mediated response was observed in mycophenolate-treated patients. Positive delta-SARS-CoV-2 NT Abs levels were detected in almost all the SARS-CoV-2 recovered subjects but not in previously uninfected patients. Our study supports previous observations of a low level of seroconversion after vaccination in transplanted patients.
Special Issue “Advanced Techniques and Efficiency Assessment of Mechanical Processing”—Editorial Note and Critical Review of the Problems
Authors: Saramak Daniel
Published: December 2021

The value chain of metal production consists of a number of processing steps that result in obtaining the final metal product from the given raw material [...]
The Separation of Carbonaceous Matter from Refractory Gold Ore Using Multi-Stage Flotation: A Case Study
Authors: Lee Sugyeong , Gibson Charlotte E. , Ghahreman Ahmad
Published: December 2021

As a pre-treatment method of refractory gold ore, carbonaceous matter (C-matter) flotation was investigated with multi-stage flotation by rougher, scavenger, and cleaner stages. Different dosages of kerosene and MIBC (4-Methyl-2-pentanol) were applied and the optimum dosage was selected by testing in each flotation stage. With the combination of each stage, four circuit designs were suggested, which were a single-stage rougher flotation (R), rougher-scavenger flotation (R+S), rougher-scavenger-scavenger cleaner flotation (R+S+SC), and rougher-rougher cleaner-scavenger-scavenger cleaner flotations (R+S+RC+SC). The results indicated that the scavenger flotation increased C-matter recovery but reduced C-matter grade compared with single-stage rougher flotation. Cleaning of the scavenger flotation concentrate improved C-matter grade significantly, but reduced recovery slightly. Cleaning of the rougher flotation concentrate achieved overall improved selectivity in flotation. A combination of rougher-scavenger flotation followed by cleaning of both concentrates (R+S+RC+SC) resulted in 73% C-matter recovery and a combined cleaner concentrate grade of 4%; the final tailings C-matter grade was 0.9%, where the C-matter remaining in the tailings was locked, and fine grained. The results demonstrate the need for the multi-stage flotation of C-matter from refractory gold ore to achieve selective separation and suggested the potential of C-matter flotation as the pre-treatment for efficient gold production.
Beneficiation of Low-Grade Rare Earth Ore from Khalzan Buregtei Deposit (Mongolia) by Magnetic Separation
Authors: Park Ilhwan , Kanazawa Yuki , Sato Naoya , Galtchandmani Purevdelger , Jha Manis Kumar , Tabelin Carlito Baltazar , Jeon Sanghee , Ito Mayumi , Hiroyoshi Naoki
Published: December 2021

The global demand for rare earth elements (REEs) is expected to increase significantly because of their importance in renewable energy and clean storage technologies, which are critical for drastic carbon dioxide emission reduction to achieve a carbon-neutral society. REE ore deposits around the world are scarce and those that have been identified but remain unexploited need to be developed to supply future demands. In this study, the Khalzan Buregtei deposit located in western Mongolia was studied with the aim of upgrading low-grade REE ore via magnetic separation techniques. The total REE content in this ore was ~6720 ppm (~3540 ppm light REE (LREE) + ~3180 ppm heavy REE (HREE)) with bastnaesite, pyrochlore, synchysite, and columbite-(Fe) identified as the main REE-bearing minerals. As the particle size fraction decreased from −4.0 + 2.0 mm to −0.5 + 0.1 mm, the recovery by dry high-intensity magnetic separation (DHIMS) increased from 20% to 70% of total rare earth oxide (TREO) while the enrichment ratio reached 2.8 from 1.3. Although effective, gangue minerals such as quartz and aluminosilicates were recovered (~22%) due most likely to insufficient liberation. Meanwhile, the wet high-intensity magnetic separation (WHIMS) could produce a magnetic concentrate with TREO recovery of ~80% and enrichment ratio of 5.5 under the following conditions: particle size fraction, −106 + 75 μm; feed flow rate, 3.2 L/min; magnetic induction, 0.8 T. These results indicate that combining DHIMS and WHIMS to upgrade the low-grade REE ore from the Khalzan Buregtei deposit is an effective approach.
Caffeine Induces G0/G1 Cell Cycle Arrest and Inhibits Migration through Integrin αv, β3, and FAK/Akt/c-Myc Signaling Pathway
Authors: Meisaprow Pichitchai , Aksorn Nithikoon , Vinayanuwattikun Chanida , Chanvorachote Pithi , Sukprasansap Monruedee
Published: December 2021

Lung cancer is recognized as a major cause of mortality worldwide owing to its metastatic activity. Given the lack of solid information regarding the possible effects of caffeine, one of the most consumed natural psychoactive substances, on molecular signaling pathways implicated in the aggressive behavior of lung cancer, our study aimed to evaluate the effect and mechanism of caffeine on metastasis-related mechanisms. The results revealed that caffeine treatment at concentrations of 0–500 µM caused no direct cytotoxic effects on NCI-H23 cells. Treatment of cells with caffeine showed good potential to inhibit cell proliferation at 48 h and induced significant cell cycle arrest at the G0/G1 phase. Concerning metastasis, caffeine was shown to reduce filopodia formation, inhibit migration and invasion capability, and reduce the ability of cancer cells to survive and grow in an anchorage-independent manner. Moreover, caffeine could attenuate the formation of 3D tumor spheroids in cancer stem cell (CSC)-enriched populations. With regard to mechanisms, we found that caffeine significantly altered the integrin pattern of the treated cells and caused the downregulation of metastasis-associated integrins, namely, integrins αv and β3. Subsequently, the downstream signals, including protein signaling and transcription factors, namely, phosphorylated focal adhesion kinase (p-FAK), phosphorylated protein kinase B (p-Akt), cell division cycle 42 (Cdc42), and c-Myc, were significantly decreased in caffeine-exposed cells. Taken together, our novel data on caffeine-inhibiting mechanism in relation to metastasis in lung cancer could provide insights into the impact of caffeine intake on human diseases and conditions.
Nanocellulose Hybrid Lignin Complex Reinforces Cellulose to Form a Strong, Water-Stable Lignin–Cellulose Composite Usable as a Plastic Replacement
Authors: Bai Feitian , Dong Tengteng , Chen Wei , Wang Jinlong , Li Xusheng
Published: December 2021

The significant challenges in the use of cellulose as a replacement for plastic are its mechanical properties’ degradation and uncontrolled deformation during the rewetting process. Herein, inspired by the reinforcement of cellulose by lignin in natural plant tissue, a strong and water-stable lignin–cellulose composite (LCC) was developed. A nanocellulose hybrid lignin complex (CHLC) created from bagasse residue after enzymatic hydrolysis was added into a pulp of bleached fibre extracted from pine to produce a lignin–cellulose sheet. The lignin as a water-stable reinforcing matrix, via the hydrogen bonding of the nanocellulose in the CHLC with the fibre was efficiently introduced onto the fibres and the fibre network voids. Compared with a typical lignin-free cellulose sheet, the dry strength and wet strength of the LCC were 218% and 2233% higher, respectively. The developed LCC is an eco-friendly and biodegradable alternative to plastic.
Recycling Rusty Iron with Natural Zeolite Heulandite to Create a Unique Nanocatalyst for Green Hydrogen Production
Authors: Shaban Mohamed , BinSabt Mohammad , Ahmed Ashour M. , Mohamed Fatma
Published: December 2021

Corrosion-induced iron rust causes severe danger, pollution, and economic problems. In this work, nanopowders of Fe2O3 and Fe2O3/zeolite are synthesized for the first time using rusted iron waste and natural zeolite heulandite by chemical precipitation. The chemical composition, nanomorphologies, structural parameters, and optical behaviors are investigated using different techniques. The Fe2O3/zeolite nanocomposite showed smaller sizes and greater light absorption capability in visible light than Fe2O3 nanopowder. The XRD pattern shows crystalline hematite (α-Fe2O3) with a rhombohedral structure. The crystallite sizes for the plane (104) of the Fe2O3 and Fe2O3/zeolite are 64.84 and 56.53 nm, respectively. The Fe2O3 and Fe2O3/zeolite have indirect bandgap values of 1.87 and 1.91 eV and direct bandgap values of 2.04 and 2.07 eV, respectively. Fe2O3 and Fe2O3/zeolite nanophotocatalysts are used for solar photoelectrochemical (PEC) hydrogen production. The Fe2O3/zeolite exhibits a PEC catalytic hydrogen production rate of 154.45 mmol/g.h @ 1 V in 0.9 M KOH solution, which is the highest value yet for Fe2O3-based photocatalysts. The photocurrent density of Fe2O3/zeolite is almost two times that of Fe2O3 catalyst, and the IPCE (incident photon-to-current conversion efficiency) reached ~27.34%@307 nm and 1 V. The electrochemical surface area (ECSA) values for Fe2O3 and Fe2O3/zeolite photocatalysts were 7.414 and 21.236 m2/g, respectively. The rate of hydrogen production for Fe2O3/zeolite was 154.44 mmol h−1/g. This nanophotocatalyst has a very low PEC corrosion rate of 7.6 pm/year; it can retain ~97% of its initial performance. Therefore, the present research can be applied industrially as a cost-effective technique to address two issues at once by producing solar hydrogen fuel and recycling the rusted iron wires.
Observation of Cu Spin Fluctuations in High-Tc Cuprate Superconductor Nanoparticles Investigated by Muon Spin Relaxation
Authors: Winarsih Suci , Budiman Faisal , Tanaka Hirofumi , Adachi Tadashi , Koda Akihiro , Horibe Yoichi , Kurniawan Budhy , Watanabe Isao , Risdiana Risdiana
Published: December 2021

The nano-size effects of high-Tc cuprate superconductor La2−xSrxCuO4 with x = 0.20 are investigated using X-ray diffractometry, Transmission electron microscopy, and muon-spin relaxation (μSR). It is investigated whether an increase in the bond distance of Cu and O atoms in the conducting layer compared to those of the bulk state might affect its physical and magnetic properties. The μSR measurements revealed the slowing down of Cu spin fluctuations in La2−xSrxCuO4 nanoparticles, indicating the development of a magnetic correlation at low temperatures. The magnetic correlation strengthens as the particle size reduces. This significantly differs from those observed in the bulk form, which show a superconducting state below Tc. It is indicated that reducing the particle size of La2−xSrxCuO4 down to nanometer size causes the appearance of magnetism. The magnetism enhances with decreasing particle size.
Azelaic Acid Esters as Pluripotent Immunomodulatory Molecules: Nutritional Supplements or Drugs
Authors: Izbicka Elzbieta , Streeper Robert T.
Published: December 2021

Azelaic acid and its esters, the azelates, occur naturally in organisms ranging from plants to humans. We have shown that diethyl azelate (DEA) exhibits a broad range of immunomodulatory activities in vitro and in vivo, and mitigates insulin resistance. To further investigate the therapeutic utility of DEA, we evaluated its mutagenicity in Salmonella typhimurium strains, examined metabolism of DEA in rat, dog, monkey and human primary hepatocytes and in human saliva, determined pharmacokinetics of DEA after an oral dose in rats, and queried its physicochemical properties for drug-like characteristics. DEA was not mutagenic in bacterial strains ± rat liver metabolic activation system S-9. It was chemically unstable in hepatocyte culture medium with a half-life of <1 h and was depleted by the hepatocytes in <5 min, suggesting rapid hepatic metabolism. DEA was also quickly degraded by human saliva in vitro. After an oral administration of DEA to rats, the di- and monoester were undetectable in plasma while the levels of azelaic acid increased over time, reached maximum at <2 h, and declined rapidly thereafter. The observed pharmacological properties of DEA suggest that it has value both as a drug or a nutritional supplement.
Self-Control at Meals in Order Not to Gain Weight and Its Relationship with Food Cravings in Low-Carb Dieters
Authors: Oliveira Jônatas , Cordás Táki Athanássios
Published: December 2021

Aims: This study’s objective was to verify the level of food cravings and cognitive restraint in women who reported having followed a low-carb diet. Methods: The volunteers filled out the binge eating scale, the cognitive restraint subscale, the food cravings trait and state questionnaires, and the food frequency questionnaire. This study has also compared participants according to the self-reported practice of a low-carb diet. Parametric tests were used to compare the groups and Pearson’s correlations between some variables of interest (p > 0.05 adopted). Results: According to the question regarding the practice of the low-carb diet, 39 participants had tried a low-carb diet in the last six months (46.2% of these with binge eating) and 48 did not (16.7% with binge eating). Dieters showed higher levels of binge eating, cognitive restriction, and food cravings (trait and state). Dieters consumed less rice and bread but did not present lower consumption of sweets. Correlations showed that for dieters who ‘Consciously hold back at meals in order not to gain weight’, it was negatively associated with ‘Intention and plans to eat’, ‘positive reinforcement’ and ‘relieving negative states’ and food cravings scores. Conclusions: Despite the higher levels of cognitive restriction in dieters, they are negatively correlated with food craving factors and negatively associated with the consumption of sweets.
Potential of Fatty Acid Amide Hydrolase (FAAH), Monoacylglycerol Lipase (MAGL), and Diacylglycerol Lipase (DAGL) Enzymes as Targets for Obesity Treatment: A Narrative Review
Authors: Matheson Justin , Zhou Xin Ming Matthew , Bourgault Zoe , Le Foll Bernard
Published: December 2021

The endocannabinoid system (ECS) plays an integral role in maintaining metabolic homeostasis and may affect hunger, caloric intake, and nutrient absorption. Obesity has been associated with higher levels of the endogenous cannabinoid transmitters (endocannabinoids). Therefore, the ECS is an important target in obesity treatment. Modulating the enzymes that synthesize and degrade endocannabinoids, namely fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MAGL), and diacylglycerol lipase (DAGL), may be a promising strategy to treat obesity. This review aims to synthesize all studies investigating pharmacological or genetic manipulation of FAAH, MAGL, or DAGL enzymes in association with obesity-related measures. Pharmacological inhibition or genetic deletion of FAAH tended to promote an obesogenic state in animal models, though the relationships between human FAAH polymorphisms and obesity-related outcomes were heterogeneous, which could be due to FAAH having both pro-appetitive and anti-appetitive substrates. Genetic deletion of Mgll and Dagla as well as pharmacological inhibition of DAGL tended to reduce body weight and improve metabolic state in animal studies, though the effects of Mgll manipulation were tissue-dependent. Monitoring changes in body weight in ongoing clinical trials of FAAH inhibitors may clarify whether FAAH inhibition is a potential therapeutic strategy for treatment obesity. More preclinical work is needed to characterize the role of MAGL and DAGL modulation in obesity-related outcomes.
Quality Assessment of Investigational Medicinal Products in COVID-19 Clinical Trials: One Year of Activity at the Clinical Trials Office
Authors: Dri Diego Alejandro , Praticò Giulia , Gaucci Elisa , Marianecci Carlotta , Gramaglia Donatella
Published: December 2021

One year after the spread of the pandemic, we analyzed the assessment results of the quality documentation submitted to the Clinical Trials Office of the Italian Medicines Agency as part of the request for authorization of clinical trials with a COVID-19 indication. In this article, we report the classification of the documentation type, an overview of the assessment results, and the related issues focusing on the most frequently detected ones. Relevant data regarding the Investigational Medicinal Products (IMPs) tested in COVID-19 clinical trials and their quality profiles are provided in the perspective of increasing transparency and availability of information. Some criticalities that have been exacerbated by the management of clinical trials during the emergency period are highlighted. Results confirm that IMPs tested in authorized COVID-19 clinical trials are developed in agreement with the same legal requirements for quality, safety, and efficacy as for any other medicinal product in the European Union (EU). The same strong regulatory framework applies, and there is no lowering in the safety profile due to the pandemic; authorized IMPs meet the highest standards of quality. The regulatory network should capitalize on lessons learned from the emergency setting. Some take-home messages are provided that could support the regulatory framework to expand its boundaries by innovating and evolving even though remaining strong and effective.
A Comprehensive Analysis of the Thrombin Binding Aptamer Containing Functionalized Pyrrolo-2’-deoxycytidines
Authors: Kotkowiak Weronika , Jahnz-Wechmann Zofia , Pasternak Anna
Published: December 2021

Aptamers constitute an answer for the growing need for targeted therapy development. One of the most well-known representatives of this group of compounds is thrombin binding aptamers (TBA) targeted towards thrombin. The TBA inhibitory activity is determined by its spatial arrangement, which consists of two G-tetrads linked by two shorter TT loops and one longer TGT loop and folds into a unimolecular, antiparallel G-quadruplex structure. Interesting properties of the aptamer can be further improved via the introduction of a number of chemical modifications. Herein, a comprehensive analysis of the influence of pyrrolo-2’-deoxycytidine (Py-dC) and its derivatives on TBA physicochemical and biological properties has been presented. The studies have shown that the presence of modified residues at the T7 position of the TGT loop has only minor effects on TBA thermodynamic stability without affecting its folding topology. All analyzed oligomers exhibit anticoagulant properties, but only aptamer modified with a decyl derivative of Py-dC was able to inhibit thrombin activity more efficiently than unmodified, parental compounds. Importantly, the same compound also possessed the potential to effectively restrain HeLa cell line growth.
Unveiling the Membrane and Cell Wall Action of Antimicrobial Cyclic Lipopeptides: Modulation of the Spectrum of Activity
Authors: Segovia Roser , Solé Judith , Marqués Ana Maria , Cajal Yolanda , Rabanal Francesc
Published: December 2021

Antibiotic resistance is a major public health challenge, and Gram-negative multidrug-resistant bacteria are particularly dangerous. The threat of running out of active molecules is accelerated by the extensive use of antibiotics in the context of the COVID-19 pandemic, and new antibiotics are urgently needed. Colistin and polymyxin B are natural antibiotics considered as last resort drugs for multi-resistant infections, but their use is limited because of neuro- and nephrotoxicity. We previously reported a series of synthetic analogues inspired in natural polymyxins with a flexible scaffold that allows multiple modifications to improve activity and reduce toxicity. In this work, we focus on modifications in the hydrophobic domains, describing analogues that broaden or narrow the spectrum of activity including both Gram-positive and Gram-negative bacteria, with MICs in the low µM range and low hemolytic activity. Using biophysical methods, we explore the interaction of the new molecules with model membranes that mimic the bacterial inner and outer membranes, finding a selective effect on anionic membranes and a mechanism of action based on the alteration of membrane function. Transmission electron microscopy observation confirms that polymyxin analogues kill microbial cells primarily by damaging membrane integrity. Redistribution of the hydrophobicity within the polymyxin molecule seems a plausible approach for the design and development of safer and more selective antibiotics.
PEGylated Liposomes Remotely Loaded with the Combination of Doxorubicin, Quinine, and Indocyanine Green Enable Successful Treatment of Multidrug-Resistant Tumors
Authors: Grabarnick (Portnoy) Emma , Andriyanov Alexander V. , Han Hadas , Eyal Sara , Barenholz Yechezkel
Published: December 2021

Multidrug resistance (MDR) of cancer cells remains a major obstacle to favorable outcomes of treatment with many drugs, including doxorubicin. Most of the clinical trials failed to demonstrate the benefit of the drug efflux transporter P-glycoprotein (P-gp) inhibitors to circumvent P-gp-mediated drug resistance in vivo. The present study explored the therapeutic potential of combined treatment with liposomal doxorubicin, P-gp inhibitor quinine, and the photodynamic therapy (PDT) using indocyanine green (ICG) in the adenocarcinoma drug-resistant tumor model. Liposomes were actively co-remotely loaded with doxorubicin and quinine, and ICG was passively adsorbed. The liposomes were characterized by differential scanning calorimetry (DSC) and cryogenic transmission microscopy (Cryo-TEM). We found that quinine impaired the crystalline structure of doxorubicin. In vitro, treatment with single agents themselves was insufficient to inhibit the growth of HT-29 MDR1 cells. However, pegylated liposomal doxorubicin and quinine (PLDQ) significantly diminished HT-29 MDR1 cell survival. Furthermore, survival inhibition intensified by the addition of ICG to the PLDQ (ICG + PLDQ). In vivo, ICG + PLDQ significantly decreased tumor growth when combined with tumor irradiation with NIR light (** p < 0.01). ICG + PLDQ + irradiation was superior to single treatments or combinational treatments without irradiation. These findings suggest that ICG + PLDQ can overcome P-gp-mediated MDR in cancer cells.
Non-Cationic RGD-Containing Protein Nanocarrier for Tumor-Targeted siRNA Delivery
Authors: Yu Xiaolin , Xue Lu , Zhao Jing , Zhao Shuhua , Wu Daqing , Liu Hong Yan
Published: December 2021

Despite the recent successes in siRNA therapeutics, targeted delivery beyond the liver remains the major hurdle for the widespread application of siRNA in vivo. Current cationic liposome or polymer-based delivery agents are restricted to the liver and suffer from off-target effects, poor clearance, low serum stability, and high toxicity. In this study, we genetically engineered a non-cationic non-viral tumor-targeted universal siRNA nanocarrier (MW 26 KDa). This protein nanocarrier consists of three function domains: a dsRNA binding domain (dsRBD) (from human protein kinase R) for any siRNA binding, 18-histidine for endosome escape, and two RGD peptides at the N- and C-termini for targeting tumor and tumor neovasculature. We showed that cloned dual-RGD-dsRBD-18his (dual-RGD) protein protects siRNA against RNases, induces effective siRNA endosomal escape, specifically targets integrin αvβ3 expressing cells in vitro, and homes siRNA to tumors in vivo. The delivered siRNA leads to target gene knockdown in the cell lines and tumor xenografts with low toxicity. This multifunctional and biomimetic siRNA carrier is biodegradable, has low toxicity, is suitable for mass production by fermentation, and is serum stable, holding great potential to provide a widely applicable siRNA carrier for tumor-targeted siRNA delivery.
The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line
Authors: El-aziz Enas Alaa El-din Abd , Elgayar Sherif Farouk , Mady Fatma M. , Abourehab Mohammed A. S. , Hasan Omiya Ali , Reda Lamis M. , Alaaeldin Eman
Published: December 2021

Purpose: Development of pharmaceutical dosage forms of natural products has gained great interest recently. Propolis is a natural product with various active compounds and multiple pharmacological activities. Its resinous nature and low bioavailability were obstacles in the optimum use of this magnificent natural product. Aim: This study evaluates the effect of using liposomes as a drug delivery system on the enhancement of the cytotoxic effect of propolis on squamous cell carcinoma cell lines (Hep-2) of head and neck. Methods: An optimized liposomal formulation of propolis was prepared using the conventional thin film hydration method 1, 2. The prepared (Hep-2) cell line was treated with different concentrations of propolis and optimized propolis liposomes for 24 h. The effect of both propolis and propolis liposomes on cell line was investigated using MTT assay, cytological examination, and nuclear morphometric analysis. The effect of the drugs on the cell apoptosis was evaluated using Annexin V. Results: The findings revealed that both propolis and propolis liposomes have a cytotoxic effect on Hep-2 cell line through induction of apoptosis. The effect was dose dependent. However, a statistically significant enhancement in propolis-mediated apoptosis on Hep-2 cells was elucidated due to encapsulation within the prepared liposomes. Conclusion: Liposome is a powerful tool for enhancing the cytotoxicity of propolis against Hep-2 cell line.
Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders
Authors: Laurent Alexis , Porcello Alexandre , Fernandez Paula Gonzalez , Jeannerat Annick , Peneveyre Cédric , Abdel-Sayed Philippe , Scaletta Corinne , Hirt-Burri Nathalie , Michetti Murielle , de Buys Roessingh Anthony , Raffoul Wassim , Allémann Eric , Jordan Olivier , Applegate Lee Ann
Published: December 2021

Cultured progenitor cells and derivatives have been used in various homologous applications of cutaneous and musculoskeletal regenerative medicine. Active pharmaceutical ingredients (API) in the form of progenitor cell derivatives such as lysates and lyophilizates were shown to retain function in controlled cellular models of wound repair. On the other hand, hyaluronan-based hydrogels are widely used as functional vehicles in therapeutic products for tendon tissue disorders. The aim of this study was the experimental characterization of formulations containing progenitor tenocyte-derived APIs and hyaluronan, for the assessment of ingredient compatibility and stability in view of eventual therapeutic applications in tendinopathies. Lyophilized APIs were determined to contain relatively low quantities of proteins and growth factors, while being physicochemically stable and possessing significant intrinsic antioxidant properties. Physical and rheological quantifications of the combination formulas were performed after hydrogen peroxide challenge, outlining significantly improved evolutive viscoelasticity values in accelerated degradation settings. Thus, potent effects of physicochemical protection or stability enhancement of hyaluronan by the incorporated APIs were observed. Finally, combination formulas were found to be easily injectable into ex vivo tendon tissues, confirming their compatibility with further translational clinical approaches. Overall, this study provides the technical bases for the development of progenitor tenocyte derivative-based injectable therapeutic products or devices, to potentially be applied in tendinous tissue disorders.
The Role of Transmission Electron Microscopy in the Early Development of Mesoporous Materials for Tissue Regeneration and Drug Delivery Applications
Authors: Ruiz-González María Luisa , Torres-Pardo Almudena , González-Calbet José M.
Published: December 2021

For the last 20 years, silica-based mesoporous materials have provided a sound platform for the development of biomedical technology applied to tissue engineering and drug delivery. Their unique structural and textural characteristics, chiefly, the ordered distribution of homogeneous and tunable pores with high surface areas and large pore volume, and their excellent biocompatibility provide an excellent starting point for bone tissue regeneration on the mesoporous surface, and also to load species of interest inside the pores. Adequate control of the synthesis conditions and functionalization of the mesoporous surface are critical factors in the design of new systems that are suitable for use in specific medical applications. Simultaneously, the use of appropriate characterization techniques in the several stages of design and manufacture of mesoporous particles allows us to ascertain the textural, structural and compositional modifications induced during the synthesis, functionalization and post-in vitro assays processes. In this scenario, the present paper shows, through several examples, the role of transmission electron microscopy and associated spectroscopic techniques in the search for useful information in the early design stages of mesoporous systems, with application in the fields of tissue regeneration and drug delivery systems.
Force Dependent Quantum Phase Transition in the Hybrid Optomechanical System
Authors: Li Lingchao , Zhang Jian-Qi
Published: December 2021

The optomechanics shows a great potential in quantum control and precise measurement due to appropriate mechanical control. Here we theoretically study the quantum phase transition in a hybrid atom-optomechanical cavity with an external force. Our study shows, in the thermodynamic limit, the critical value of quantum phase transition between the normal phase and super-radiant phase can be controlled and modified by the external force via the tunable frequency of optomechanics, then a force dependent quantum phase transition can be achieved in our system. Moreover, this force dependent quantum phase transition can be employed to detect the external force variation. In addition, our numerical simulations illustrate the sensitivity of the external force measurement can be improved by the squeezing properties of the quantum phase transition.
The Patterns of Male and Female Flowers in Flowering Stage May Not Be Optimal Resource Allocation for Fruit and Seed Growth
Authors: Gao Lei , Yu Guozhu , Hu Fangyu , Li Zhiqi , Li Weihua , Peng Changlian
Published: December 2021

Changes in the proportions of male and female flowers in monoecious plants in response to external environmental conditions are directly related to the reproductive fitness of plants. The monoecious cucumber (Cucumber sativus) plant was used in this study to assess the responses of sex differentiation and the breeding process to nutrient supply and the degree of artificial pollination using pollen solutions of different concentrations. We found that the nutrient supply significantly improved the number of female flowers, while pollination treatments did not obviously increase the number of male flowers. Continuous pollination changed the number of female flowers especially in the later stage of the pollination experiment. Therefore, pollination changed the ratio of male and female flowers in the flowering stage of cucumber. Pollination treatment affected the fruit growth, seed set, and fruit yield. The number of fruit, fruit set percentage, and total seeds per plant did not increase with the pollination level, but individual fruit weight and seed number in one fruit did increase. The differentiation of male and female flowers in the flowering stage of cucumber is a response to nutrient and pollination resources, but this response is not the optimal resource allocation for subsequent fruit development and seed maturity, which suggests that the response of plants to external environment resources is short-term and direct.
Exogenous Glycine Betaine Application Improves Freezing Tolerance of Cabbage (Brassica oleracea L.) Leaves
Authors: Min Kyungwon , Cho Yunseo , Kim Eunjeong , Lee Minho , Lee Sang-Ryong
Published: December 2021

Exogenous glycine betaine (GB) application has been reported to improve plant tolerance to various abiotic stresses, but its effect on freezing tolerance has not been well studied. We investigated the effect of exogenous GB on freezing tolerance of cabbage (Brassica oleracea L.) leaves. Seedlings fed with 30 mM GB via sub-irrigation showed effectively assimilated GB as evident by higher GB concentration. Exogenous GB did not retard leaf-growth (fresh weight, dry weight, and leaf area) rather slightly promoted it. Temperature controlled freeze-thaw tests proved GB-fed plants were more freeze-tolerant as indicated by lower electrolyte leakage (i.e., indication of less membrane damage) and alleviating oxidative stress (less accumulation of O2•− and H2O2, as well as of malondialdehyde (MDA)) following a relatively moderate or severe freeze-thaw stress, i.e., −2.5 and −3.5 °C. Improved freezing tolerance induced by exogenous GB application may be associated with accumulation of compatible solute (proline) and antioxidant (glutathione). GB-fed leaves also had higher activity of antioxidant enzymes, catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD). These changes, together, may improve freezing tolerance through membrane protection from freeze-desiccation and alleviation of freeze-induced oxidative stress.
Characterization of Polyhydroxybutyrate-Based Composites Prepared by Injection Molding
Authors: Hernandez Marcos M. , Gupta Nevin S. , Lee Kwan-Soo , Pital Aaron C. , Marrone Babetta L. , Iverson Carl N. , Dumont Joseph H.
Published: December 2021

The waste generated by single-use plastics is often non-recyclable and non-biodegradable, inevitably ending up in our landfills, ecosystems, and food chain. Through the introduction of biodegradable polymers as substitutes for common plastics, we can decrease our impact on the planet. In this study, we evaluate the changes in mechanical and thermal properties of polyhydroxybutyrate-based composites with various additives: Microspheres, carbon fibers or polyethylene glycol (2000, 10,000, and 20,000 MW). The mixtures were injection molded using an in-house mold attached to a commercial extruder. The resulting samples were characterized using microscopy and a series of spectroscopic, thermal, and mechanical techniques. We have shown that the addition of carbon fibers and microspheres had minimal impact on thermal stability, whereas polyethylene glycol showed slight improvements at higher molecular weights. All of the composite samples showed a decrease in hardness and compressibility. The findings described in this study will improve our understanding of polyhydroxybutyrate-based composites prepared by injection molding, enabling advancements in integrating biodegradable plastics into everyday products.
New Considerations on the Determination of the Apparent Shear Viscosity of Polymer Melt with Micro Capillary Dies
Authors: Wu Wangqing , Zeng Ke , Zhao Baishun , Duan Fengnan , Jiang Fengze
Published: December 2021

Capillary rheometers have been widely used for the rheological measurement of polymer melts. However, when micro capillary dies are used, the results are usually neither accurate nor consistent, even under the same measurement conditions. In this work, theoretical modeling and experimental studies were conducted for a more profound understanding of the mechanism by which the initial and boundary conditions influence the inaccuracy in the apparent shear viscosity determination with micro capillary dies (diameters: 500 μm, 200 μm, 100 μm). The results indicate that the amount of polymer initially in the barrel, the pre-compaction pressure and the capillary die diameter have a significant influence on the development of the micro scale inlet pressure, which directly determines the accuracy of the measurement at low and medium shear rates. The varying melt compressibility was confirmed to be the main factor directly related to the inaccuracy in the micro scale apparent shear viscosity determination. It is suggested that measures such as reducing the amount of polymer initially in the barrel and increasing the pre-compaction pressure could be used to reduce the measurement inaccuracy.
Hydrogels Based on Alginates and Carboxymethyl Cellulose with Modulated Drug Release—An Experimental and Theoretical Study
Authors: Peptu Cătălina Anișoara , Băcăiță Elena Simona , Savin (Logigan) Corina-Lenuta , Luțcanu Marian , Agop Maricel
Published: December 2021

New hydrogels films crosslinked with epichlorohydrin were prepared based on alginates and carboxymethyl cellulose with properties that recommend them as potential drug delivery systems (e.g., biocompatibility, low toxicity, non-immunogenicity, hemostatic activity and the ability to absorb large amounts of water). The characterization of their structural, morphological, swelling capacity, loading/release and drug efficiency traits proved that these new hydrogels are promising materials for controlled drug delivery systems. Further, a new theoretical model, in the framework of Scale Relativity Theory, was built with to offer insights on the release process at the microscopic level and to simplify the analysis of the release process.
Manufacturing and Characterization of Environmentally Friendly Wood Plastic Composites Using Pinecone as a Filler into a Bio-Based High-Density Polyethylene Matrix
Authors: Morcillo Maria del Carmen , Tejada Ramón , Lascano Diego , Garcia-Garcia Daniel , Garcia-Sanoguera David
Published: December 2021

The use of wood plastic composites (WPC) is growing very rapidly in recent years, in addition, the use of plastics of renewable origin is increasingly implemented because it allows to reduce the carbon footprint. In this context, this work reports on the development of composites of bio-based high density polyethylene (BioHDPE) with different contents of pinecone (5, 10, and 30 wt.%). The blends were produced by extrusion and injection-molded processes. With the objective of improving the properties of the materials, a compatibilizer has been used, namely polyethylene grafted with maleic anhydride (PE-g-MA 2 phr). The effect of the compatibilizer in the blend with 5 wt.% has been compared with the same blend without compatibilization. Mechanical, thermal, morphological, colorimetric, and wettability properties have been analyzed for each blend. The results showed that the compatibilizer improved the filler–matrix interaction, increasing the ductile mechanical properties in terms of elongation and tensile strength. Regarding thermal properties, the compatibilizer increased thermal stability and improved the behavior of the materials against moisture. In general, the pinecone materials obtained exhibited reddish-brown colors, allowing their use as wood plastic composites with a wide range of properties depending on the filler content in the blend.
Rice Hull-Derived Carbon for Supercapacitors: Towards Sustainable Silicon-Carbon Supercapacitors
Authors: Li Changwei , Chen Honglei , Zhang Liqiong , Jiao Shenghui , Zhang Huixin , Zhang Junliu , Li Peng , Tao Yubo , Zhao Xin
Published: December 2021

A simple and effective mixing carbonization-activation process was developed to prepare rice hull-derived porous Si–carbon materials. The morphologies and pore structures of the materials were controlled effectively without any loading or additions at various carbonization temperatures. The structures of the samples changed from large pores and thick walls after 800 ∘C carbonization to small pores and thin walls after 1000 ∘C carbonization. An additional alkali activation–carbonization process led to coral reef-like structures surrounded by squama in the sample that underwent 900 ∘C carbonization (Act-RH-900). This optimal material (Act-RH-900) had a large specific surface area (768 m2 g−1), relatively stable specific capacitance (150.8 F g−1), high energy density (31.9 Wh kg−1), and high-power density (309.2 w kg−1) at a current density of 0.5 A g−1 in 1 M KOH electrolyte, as well as a good rate performance and high stability (capacitance retention > 87.88% after 5000 cycles). The results indicated that Act-RH-900 is a promising candidate for capacitive applications. This work overcomes the restrictions imposed by the complex internal structure of biomass, implements a simple reaction environment, and broadens the potential applicability of biomass waste in the field of supercapacitors.
Development of Cerium Oxide/Corncob Nanocomposite: A Cost-Effective and Eco-Friendly Adsorbent for the Removal of Heavy Metals
Authors: Gran Sidra , Aziz Rukhsanda , Rafiq Muhammad Tariq , Abbasi Maryam , Qayyum Abdul , Elnaggar Ashraf Y. , Elganzory Hussein H. , El-Bahy Zeinhom M. , Hussein Enas E.
Published: December 2021

This research aims to assess the efficiency of the synthesized corncob as a cost-effective and eco-friendly adsorbent for the removal of heavy metals. Therefore, to carry out the intended research project, initially, the corncob was doped with nanoparticles to increase its efficiency or adsorption capacity. The prepared adsorbent was evaluated for the adsorption of cadmium (Cd) and chromium (Cr) from aqueous media with the batch experiment method. Factors that affect the adsorption process are pH, initial concentration, contact time and adsorbent dose. The analysis of Cd and Cr was performed by using atomic absorption spectrometry (AAS), while the characterization of the adsorbent was performed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The results showed that there is a significant difference before and after corncob activation and doping with CeO2 nanoparticles. The maximum removal for both Cd and Cr was at a basic pH with a contact time of 60 min at 120 rpm, which is 95% for Cd and 88% for Cr, respectively. To analyze the experimental data, a pseudo-first-order kinetic model, pseudo-second-order kinetic model, and intra-particle diffusion model were used. The kinetic adsorption studies confirmed that the experimental data were best fitted with the pseudo-second-order kinetic model (R2 = 0.989) and intra-particle diffusion model (R2 = 0.979). This work demonstrates that the cerium oxide/corncob nanocomposite is an inexpensive and environmentally friendly adsorbent for the removal of Cd and Cr from wastewater.