bluuebunny/crossref_metadata_2025 huggingface dataset:
SARS-CoV2 mRNA Vaccine-Specific B-, T- and Humoral Responses in Adolescents After Kidney Transplantation
Authors: Sattler Arne , Thumfart Julia , Tóth Laura , Schrezenmeier Eva , Proß Vanessa , Stahl Carolin , Siegle Janine , He An , Thole Linda Marie Laura , Ludwig Carolin , Straub-Hohenbleicher Henriette , Friedersdorff Frank , Jahrsdörfer Bernd , Schrezenmeier Hubert , Bufler Philip , Kotsch Katja
Published: August 2022

Protection of adult kidney transplant recipients against SARS-CoV2 was shown to be strongly impaired owing to low reactogenicity of available vaccines. So far, data on vaccination outcomes in adolescents are scarce due to later vaccination approval for this age group. We therefore comprehensively analyzed vaccination-specific humoral-, T- and B-cell responses in kidney transplanted adolescents aged 12–18 years in comparison to healthy controls 6 weeks after standard two-dose BNT162b2 (“Comirnaty”; Pfizer/BioNTech) vaccination. Importantly, 90% (18/20) of transplanted adolescents showed IgG seroconversion with 75% (15/20) developing neutralizing titers. Still, both features were significantly diminished in magnitude compared to controls. Correspondingly, spike-specific B cells were quantitatively reduced and enriched for non-isotype-class-switched IgD+27+ memory cells in patients. Whereas spike specific CD4+ T cell frequencies were similar in both groups, cytokine production and memory differentiation were significantly impaired in transplant recipients. Although our data identify limitations in all arms of vaccine-specific immunity, the majority of our adolescent patients showed robust humoral responses despite antimetabolite-based treatment being associated with poor vaccination outcomes in adults.
Building a Technology Recommender System Using Web Crawling and Natural Language Processing Technology
Authors: Campos Macias Nathalie , Düggelin Wilhelm , Ruf Yesim , Hanne Thomas
Published: August 2022

Finding, retrieving, and processing information on technology from the Internet can be a tedious task. This article investigates if technological concepts such as web crawling and natural language processing are suitable means for knowledge discovery from unstructured information and the development of a technology recommender system by developing a prototype of such a system. It also analyzes how well the resulting prototype performs in regard to effectivity and efficiency. The research strategy based on design science research consists of four stages: (1) Awareness generation; (2) suggestion of a solution considering the information retrieval process; (3) development of an artefact in the form of a Python computer program; and (4) evaluation of the prototype within the scope of a comparative experiment. The evaluation yields that the prototype is highly efficient in retrieving basic and rather random extractive text summaries from websites that include the desired search terms. However, the effectivity, measured by the quality of results is unsatisfactory due to the aforementioned random arrangement of extracted sentences within the resulting summaries. It is found that natural language processing and web crawling are indeed suitable technologies for such a program whilst the use of additional technology/concepts would add significant value for a potential user. Several areas for incremental improvement of the prototype are identified.
Increasing Environmental Complexity by Providing Different Types of Litter and Perches during Early Rearing Boosts Coping Abilities in Domestic Fowl Chicks
Authors: Nazar Franco Nicolas , Skånberg Lena , McCrea Kirste , Keeling Linda Jane
Published: August 2022

Early experience of a complex environment can improve biologically relevant traits related to coping abilities. However, the mechanisms underlying these positive effects have not been well explored. We hypothesized that giving chicks possibilities to express choices within relevant resources could be an important part of the mechanism, as well as a novel way to increase environmental complexity. In a balanced design, laying hen hatchlings of the white hybrid Bovans Robust were reared in a “single-choice” environment (single litter and perch type) or a “multi-choice” environment (four different litter and perch types). Immunological and behavioral indicators of chicks’ coping abilities were explored in this experimental study at three weeks of age. Chicks from “multi-choice” environments had shorter durations of tonic immobility, lower heterophil/lymphocyte ratios, higher natural antibody concentrations, and were more successful in gaining novel food rewards in a repeated opportunity test. These results imply that chicks having access to variation within resource types were less fearful, experienced less chronic stress, would be more able to cope with pathogenic challenges, and potentially had an improved learning ability. To conclude, the more complex environment, achieved by increasing chicks’ possibilities to choose, seemed to make chicks better prepared for potential challenges, boosting their adaptive capacities and their ability to make the most of opportunities.
Insights into the Evolution of Aphid Mitogenome Features from New Data and Comparative Analysis
Authors: Zhang Hui , Lu Congcong , Liu Qian , Zou Tianmin , Qiao Gexia , Huang Xiaolei
Published: August 2022

The complete mitochondrial genomes and their rearrangement patterns can provide useful information for inferring evolutionary history of organisms. Aphids are one of the insect groups with some unique mitogenome features. In this study, to examine whether some features in aphid mitogenomes are independent species-specific evolutionary events or clade-specific events at certain taxonomic levels, we sequenced three new aphid mitogenomes (Hormaphidinae: Ceratovacuna keduensis, Pseudoregma panicola; Lachninae: Nippolachnus piri) and compared them with all known aphid mitogenomes. The three mitogenomes are 16,059–17,033 bp in length, with a set of 37 typical mitochondrial genes, a non-coding control region and a tandem repeat region. The gene orders of them are all highly rearranged. Within the subfamily Hormaphidinae, the presence of repeat region and mitogenome rearrangement in Cerataphidini species but not in the other two tribes indicate that these may be Cerataphidini-specific features. The same gene rearrangement pattern in the two Lachninae species, N. piri (Tuberolachnini) and Stomaphis sinisalicis (Stomaphidini), supports that this feature should be at least derived from the common ancestor of two tribes. Overall, our data and analyses provide new insights into the evolutionary patterns of gene rearrangement and repeat region in aphid mitogenomes, and further corroborate the potential role of gene rearrangement in elucidating the evolutionary history of different insect lineages.
Exploring the Potential of Myrothamnus flabellifolius Welw. (Resurrection Tree) as a Phytogenic Feed Additive in Animal Nutrition
Authors: Nantapo Carlos Wyson Tawanda , Marume Upenyu
Published: August 2022

Myrothamnus flabellifolius (Welw.) is used in African traditional medicine for the treatment of depression and mental disorder, asthma, infectious diseases, respiratory, inflammation, epilepsy, heart, wound, backaches, diabetes, kidney ailments, hypertension, hemorrhoids, gingivitis, shingles, stroke, and skins conditions. The effectiveness of M. flabellifolius is due to the presence of several secondary metabolites that have demonstrated efficacy in other cell and animal models. These metabolites are key in cell regulation and function and have potential use in animal production due to antimicrobial and antioxidant properties, for an improvement in growth performance, feed quality and palatability, gut microbial environment, function, and animal health. The purpose of this review is to provide a detailed account on the potential use of M. flabellifolius in animal nutrition. Limitations towards the use of this plant in animal nutrition, including toxicity, economic, and financial issues are discussed. Finally, novel strategies and technologies, e.g., microencapsulation, microbial fermentation, and essential oil extraction, used to unlock and improve nutrient bioaccessibility and bioavailability are clearly discussed towards the potential use of M. flabellifolius as a phytogenic additive in animal diets.
Predicting Dyslexia in Adolescents from Eye Movements during Free Painting Viewing
Authors: El Hmimdi Alae Eddine , Ward Lindsey M , Palpanas Themis , Sainte Fare Garnot Vivien , Kapoula Zoï
Published: August 2022

It is known that dyslexics present eye movement abnormalities. Previously, we have shown that eye movement abnormalities during reading or during saccade and vergence testing can predict dyslexia successfully. The current study further examines this issue focusing on eye movements during free exploration of paintings; the dataset was provided by a study in our laboratory carried by Ward and Kapoula. Machine learning (ML) classifiers were applied to eye movement features extracted by the software AIDEAL: a velocity threshold analysis reporting amplitude speed and disconjugacy of horizontal saccades. In addition, a new feature was introduced that concerns only the very short periods during which the eyes were moving, one to the left the other to the right; such periods occurred mostly during fixations between saccades; we calculated a global index of the frequency of such disconjugacy segments, of their duration and their amplitude. Such continuous evaluation of disconjugacy throughout the time series of eye movements differs from the disconjugacy feature that describes inequality of the saccade amplitude between the two eyes. The results show that both AIDEAL features, and the Disconjugacy Global Index (DGI) enable successful categorization of dyslexics from non-dyslexics, at least when applying this analysis to the specific paintings used in the present study. We suggest that this high power of predictability arises from both the content of the paintings selected and the physiologic relevance of eye movement features extracted by the AIDEAL and the DGI.
Architectural Survey, Diagnostic, and Constructive Analysis Strategies for Monumental Preservation of Cultural Heritage and Sustainable Management of Tourism
Authors: Rueda Márquez de la Plata Adela , Cruz Franco Pablo Alejandro , Ramos Sánchez Jorge Alberto
Published: August 2022

Heritage is under constant pressure to be adapted for tourism. The aim of this research was to improve the performance of both the tourism sector and the cultural heritage sector in a balanced and sustainable way, by considering the principles of conservation and preservation. Non-invasive tools such as ground penetrating radar (GPR), unmanned aerial vehicle (UAV), and even virtual reality (VR) and augmented reality (AR) technologies have been used to develop new methodologies, allowing us to generate new experiences and heritage tourist attractions, which not only do not generate negative impacts on the monuments themselves, but also promote their preservation without diminishing the cultural and tourist offers of the city. A case study was carried out on Mérida, a UNESCO World Heritage City, where tourism is a strong economic engine of the city. The results obtained are two-fold: on one hand, an effective interdisciplinary working methodology for heritage management was developed, and on the other hand, new approaches for the sustainable development of cultural tourism were generated.
Transcriptomic Signature and Growth Factor Regulation of Castration-Tolerant Prostate Luminal Progenitor Cells
Authors: Baures Manon , Puig Lombardi Emilia , Di Martino Delphine , Zeitouni Wail , Pacreau Emeline , Dos Santos Leïla , Dariane Charles , Boutillon Florence , Guidotti Jacques-Emmanuel , Goffin Vincent
Published: August 2022

Background: The molecular and cellular mechanisms that drive castration-resistant prostate cancer (CRPC) remain poorly understood. LSCmed cells defines an FACS-enriched population of castration-tolerant luminal progenitor cells that has been proposed to promote tumorigenesis and CRPC in Pten-deficient mice. The goals of this study were to assess the relevance of LSCmed cells through the analysis of their molecular proximity with luminal progenitor-like cell clusters identified by single-cell (sc)RNA-seq analyses of mouse and human prostates, and to investigate their regulation by in silico-predicted growth factors present in the prostatic microenvironment. Methods: Several bioinformatic pipelines were used for pan-transcriptomic analyses. LSCmed cells isolated by cell sorting from healthy and malignant mouse prostates were characterized using RT-qPCR, immunofluorescence and organoid assays. Results: LSCmed cells match (i) mouse luminal progenitor cell clusters identified in scRNA-seq analyses for which we provide a common 15-gene signature including the previously identified LSCmed marker Krt4, and (ii) Club/Hillock cells of the human prostate. This transcriptional overlap was maintained in cancer contexts. EGFR/ERBB4, IGF-1R and MET pathways were identified as autocrine/paracrine regulators of progenitor, proliferation and differentiation properties of LSCmed cells. The functional redundancy of these signaling pathways allows them to bypass the effect of receptor-targeted pharmacological inhibitors. Conclusions: Based on transcriptomic profile and pharmacological resistance to monotherapies that failed in CRPC patients, this study supports LSCmed cells as a relevant model to investigate the role of castration-tolerant progenitor cells in human prostate cancer progression.
Targeting Protein Degradation Pathways in Tumors: Focusing on their Role in Hematological Malignancies
Authors: Wolska-Washer Anna , Smolewski Piotr
Published: August 2022

Cells must maintain their proteome homeostasis by balancing protein synthesis and degradation. This is facilitated by evolutionarily-conserved processes, including the unfolded protein response and the proteasome-based system of protein clearance, autophagy, and chaperone-mediated autophagy. In some hematological malignancies, including acute myeloid leukemia, misfolding or aggregation of the wild-type p53 tumor-suppressor renders cells unable to undergo apoptosis, even with an intact p53 DNA sequence. Moreover, blocking the proteasome pathway triggers lymphoma cell apoptosis. Extensive studies have led to the development of proteasome inhibitors, which have advanced into drugs (such as bortezomib) used in the treatment of certain hematological tumors, including multiple myeloma. New therapeutic options have been studied making use of the so-called proteolysis-targeting chimeras (PROTACs), that bind desired proteins with a linker that connects them to an E3 ubiquitin ligase, resulting in proteasomal-targeted degradation. This review examines the mechanisms of protein degradation in the cells of the hematopoietic system, explains the role of dysfunctional protein degradation in the pathogenesis of hematological malignancies, and discusses the current and future advances of therapies targeting these pathways, based on an extensive search of the articles and conference proceedings from 2005 to April 2022.
A Prospective Validation Study of Lung Cancer Gene Panel Testing Using Cytological Specimens
Authors: Morikawa Kei , Kida Hirotaka , Handa Hiroshi , Inoue Takeo , Saji Hisashi , Koike Junki , Nakamura Seiji , Sato Yoshiharu , Ueda Yumi , Suzuki Fumihiko , Matoba Ryo , Mineshita Masamichi
Published: August 2022

Background: Genetic panel tests require sufficient tissue samples, and therefore, cannot always be performed. Although collecting cytological specimens is easier than tissue collection, there are no validation studies on the diagnostic accuracy of lung cancer gene panel tests using cytology samples. Methods: Using an amplicon-based high-sensitivity next-generation sequencing panel test capable of measuring eight druggable genes, we prospectively enrolled consecutive patients who underwent diagnostic procedures. We evaluated the analysis accuracy rate, nucleic acid yield, and the quality of cytological specimens under brushing, needle aspiration, and pleural effusion. We then compared these specimens with collected tissue samples. Results: In 163 prospectively enrolled cases, nucleic acid extraction and analysis accuracy was 100% in cases diagnosed with adenocarcinoma. Gene mutations were found in 68.7% of cases with 99.5% (95% CI: 98.2–99.9) concordance to companion diagnostics. The median DNA/RNA yield and DNA/RNA integrity number were 475/321 ng and 7.9/5.7, respectively. The correlation coefficient of the gene allele ratio in 64 cases compared with tissue samples was 0.711. Conclusion: The success of gene analysis using cytological specimens was high, and the yield and quality of the extracted nucleic acid were sufficient for panel analysis. Moreover, the allele frequency of gene mutations in cytological specimens showed high correlations with tissue specimens.
Açaì (Euterpe oleracea) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress
Authors: Remigante Alessia , Spinelli Sara , Straface Elisabetta , Gambardella Lucrezia , Caruso Daniele , Falliti Giuseppe , Dossena Silvia , Marino Angela , Morabito Rossana
Published: August 2022

Aging is a process characterised by a general decline in physiological functions. The high bioavailability of reactive oxygen species (ROS) plays an important role in the aging rate. Due to the close relationship between aging and oxidative stress (OS), functional foods rich in flavonoids are excellent candidates to counteract age-related changes. This study aimed to verify the protective role of Açaì extract in a d-Galactose (d-Gal)-induced model of aging in human erythrocytes. Markers of OS, including ROS production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups, as well as the anion exchange capability through Band 3 protein (B3p) and glycated haemoglobin (A1c) have been analysed in erythrocytes treated with d-Gal for 24 h, with or without pre-incubation for 1 h with 0.5–10 µg/mL Açaì extract. Our results show that the extract avoided the formation of acanthocytes and leptocytes observed after exposure to 50 and 100 mM d-Gal, respectively, prevented d-Gal-induced OS damage, and restored alterations in the distribution of B3p and CD47 proteins. Interestingly, d-Gal exposure was associated with an acceleration of the rate constant of SO42− uptake through B3p, as well as A1c formation. Both alterations have been attenuated by pre-treatment with the Açaì extract. These findings contribute to clarify the aging mechanisms in human erythrocytes and propose functional foods rich in flavonoids as natural antioxidants for the treatment and prevention of OS-related disease conditions.
Endogenous Retroviral Sequences Behave as Putative Enhancers Controlling Gene Expression through HP1-Regulated Long-Range Chromatin Interactions
Authors: Calvet Sébastien , Sallis Séphora , Saksouk Nehmé , Rebouissou Cosette , Teyssier Catherine , Lesne Annick , Cammas Florence , Forné Thierry
Published: August 2022

About half of the mammalian genome is constituted of repeated elements, among which endogenous retroviruses (ERVs) are known to influence gene expression and cancer development. The HP1 (Heterochromatin Protein 1) proteins are known to be essential for heterochromatin establishment and function and its loss in hepatocytes leads to the reactivation of specific ERVs and to liver tumorigenesis. Here, by studying two ERVs located upstream of genes upregulated upon loss of HP1, Mbd1 and Trim24, we show that these HP1-dependent ERVs behave as either alternative promoters or as putative enhancers forming a loop with promoters of endogenous genes depending on the genomic context and HP1 expression level. These ERVs are characterised by a specific HP1-independent enrichment in heterochromatin-associated marks H3K9me3 and H4K20me3 as well as in the enhancer-specific mark H3K4me1, a combination that might represent a bookmark of putative ERV-derived enhancers. These ERVs are further enriched in a HP1-dependent manner in H3K27me3, suggesting a critical role of this mark together with HP1 in the silencing of the ERVs, as well as for the repression of the associated genes. Altogether, these results lead to the identification of a new regulatory hub involving the HP1-dependent formation of a physical loop between specific ERVs and endogenous genes.
Discovering Semantic Relations between Neurodegenerative Diseases and Artistic Behaviors
Authors: Koletis Adam , Markopoulos Athanasios , Kotis Konstantinos
Published: August 2022

This paper aims to introduce the value of semantics for representing knowledge related to patients with brain neurodegenerative diseases (Parkinson’s, Alzheimer’s, and dementia) or behavioral disorders (i.e., schizophrenia) and artistic behavior. The ultimate goal is to facilitate an effective and efficient study of neurological and behavioral changes of patients, analyzing semantically interlinked data related to neurological/behavioral conditions and artistic behaviors. By mapping the neurologically affected areas of the brain in healthy and unhealthy individuals, and by modeling their particular characteristics at the level of both behavioral and neurological expressions, it may be possible to identify semantic similarities in high-level behavioral and brain characteristics that justify correlation and causation between diseases/disorders and artistic behaviors. In this concept paper, we present our view on two key points related to proposed research on a novel framework that will (a) verify if early biomarkers of the neurogenerative diseases can be identified via artistic behavior observations, and (b) correlate patients with delayed onset of the diseases/disorders with artists, at the molecular level, or at the level of brain regions. The proposed framework is evaluated with the development of a proof-of-concept expert system based on the representation of the relevant knowledge.
Formation and Antibacterial Activity of AlOOH/Ag Composite Coating on Macroporous α-Al2O3 Ceramics
Authors: Senkina Elena , Buyakov Ales , Kazantsev Sergey , Bakina Olga , Krinitsyn Maksim , Lozhkomoev Aleksandr
Published: August 2022

In this study, the modification of macroporous α-Al2O3 ceramics with AlOOH nanostructures impregnated with silver particles is carried out using bicomponent Al/Ag nanoparticles obtained by the simultaneous electrical explosion of Al and Ag wires. Nanoparticle suspension impregnation of porous ceramics followed by oxidation with water is shown to lead to the formation of a continuous AlOOH nanosheet coating on the ceramic surface, with silver releasing on the surface of nanosheets in the form of individual particles sized 5–30 nm. Modified with AlOOH/Ag nanostructures, macroporous α-Al2O3 pellets with a diameter of 11 mm and a thickness of 5 mm show 100% efficiency for water purification from bacteria with a concentration of 105 CFU/mL for 7.5 min at a flow rate of 6.7 mL/min.
A Bayesian Motivated Two-Sample Test Based on Kernel Density Estimates
Authors: Merchant Naveed , Hart Jeffrey D.
Published: August 2022

A new nonparametric test of equality of two densities is investigated. The test statistic is an average of log-Bayes factors, each of which is constructed from a kernel density estimate. Prior densities for the bandwidths of the kernel estimates are required, and it is shown how to choose priors so that the log-Bayes factors can be calculated exactly. Critical values of the test statistic are determined by a permutation distribution, conditional on the data. An attractive property of the methodology is that a critical value of 0 leads to a test for which both type I and II error probabilities tend to 0 as sample sizes tend to ∞. Existing results on Kullback–Leibler loss of kernel estimates are crucial to obtaining these asymptotic results, and also imply that the proposed test works best with heavy-tailed kernels. Finite sample characteristics of the test are studied via simulation, and extensions to multivariate data are straightforward, as illustrated by an application to bivariate connectionist data.
Feasibility of Thermal Utilization of Primary and Secondary Sludge from a Biological Wastewater Treatment Plant in Kaliningrad City
Authors: Kulikova Yuliya , Babich Olga , Tsybina Anna , Sukhikh Stanislav , Mokrushin Ivan , Noskova Svetlana , Orlov Nikolaj
Published: August 2022

Hydrothermal liquefaction (HTL) of sewage sludge is considered in the article as an analogue of the natural processes of oil formation (catagenesis). A comparison of the physicochemical composition of primary and secondary sludge with type II kerogen (natural precursor of oil) showed their similarity. Both types of sludge have a slightly higher level of oxygen and nitrogen. The study tested the hypothesis that the elements included in the inorganic part of the oil source rocks can have a catalytic effect on the oil formation processes. For the conducted studies of sludge HTL, the catalysts containing cations and substances found in oil source rocks were chosen: as homogeneous catalysts (KOH, NaOH, NH4Fe(SO4)2, CoCl6, NiSO4, CuSO4, ZnSO4, MoO3) and as heterogeneous catalysts (MgO, Zeolite, Al2O3). The effectiveness of catalysts containing metal ions, zeolite and aluminum oxide has been proven. The highest biocrude yield was achieved in a process with NiSO4 as the catalyst in a dose 2 g per 10 g of sludge: oil yield increased by 34.9% and 63.4% in the processing of primary and secondary sludge, respectively. The use of catalysts provided an increase in fuel HHV by 10.8–12.5%, which is associated with a decrease in oxygen content (by 10.8–43.2%) with a simultaneous increase in carbon (by 7.9–10.9%) and hydrogen (by 6.5–18.7%) content.
Design of Damping Strategies for LC Filter Applied in Medium Voltage Variable Speed Drive
Authors: Gonçalves Diuary , Farias João Victor Matos , Pereira Heverton Augusto , Luiz Alex-Sander Amável , Stopa Marcelo Martins , Cupertino Allan Fagner
Published: August 2022

In recent years, medium-voltage variable-speed drives have become popular in the industry. However, in some applications, the use of long cables can lead to overvoltages at the motor terminals, affecting the motor lifespan. Under such conditions, the use of passive filters is recommended. However, the use of inductive capacitive (LC) filters results in resonances, leading to control stability issues. This issue can be mitigated by introducing a resonance damping strategy. In this work, four damping strategies are implemented and designed: Passive damping, capacitor current feedback, capacitor voltage feedback, and notch filter-based damping. The paper performs a comparative study on the current control phase margin, current and voltage harmonic spectra, and overall changes in the control structure. Then, the effect of the damping strategy on the VSD control is evaluated, creating guidelines to support the selection of the appropriate damping strategy. The results indicate that capacitor voltage feedback stands out, since this strategy presents an interesting dynamic behavior while allowing the elimination of the passive damping losses at a relatively low cost.
Effect of Different Presowing Treatments to Break Seed Dormancy and Seed Collection Methods on the Germination of Dracaena steudneri Schweinf. Ex Engl.
Authors: Munie Shiferaw Alem , Habrová Hana , Houšková Kateřina , Karas Lukáš
Published: August 2022

Research Highlights: This study is focused on the germination of Dracaena steudneri Schweinf. Ex Engl. seeds using different presowing treatments. Background and Objectives: The study aimed to overcome the problem of breaking seed dormancy, to facilitate artificial regeneration for conservation and development purposes. The objectives of this study were (1) to evaluate the effect of different seed treatments in breaking seed dormancy of D. steudneri and (2) to assess the effect of the seed collection method (seeds collected on the ground vs. from the tree) on the germination of the seed. Materials and Methods: experimental study with different seed-dormancy-breaking treatments was carried out in a greenhouse and seed laboratory. T testing and one way analysis of variance (ANOVA) were used to analyse the data. Results: The applied seed treatments (hot water, cold water, sodium hypochlorite and nicking) did not improve the germination of the species, nor the breaking of seed dormancy. One-way ANOVA results also showed no significant differences between the different seed treatments and the control on the mean germination of the species in the greenhouse (p < 0.05). The t test result also revealed no significant differences in the mean germination between fallen seeds collected from the ground and in the tree crown (p < 0.05). The tetrazolium test results showed that the percentage of nonviable seeds was greater than that of the viable seeds. Conclusion: The different treatments for breaking seed dormancy did not improve the germination of seeds in the greenhouse (ranging from 0%–7%) nor in the seed laboratory (0%), which might be due to the intermediate characteristics of the seeds of this species. Therefore, we recommend that more research is undertaken on the seed ecophysiology of the species, in order to understand the mechanisms controlling its seed germination.
How Is Virtuous Personality Trait Related to Online Deviant Behavior among Adolescent College Students in the Internet Environment? A Moderated Moderated-Mediation Analysis
Authors: Zhang Heyun , Zhao Huanhuan
Published: August 2022

Online deviant behavior is a series of maladaptive behaviors that may have adverse effects on the physical and mental health of others and adolescents in the Internet environment. Previous studies have paid more attention to the risk factors of adolescent online deviant behavior. However, its protective factors and psychological mechanisms remain unclear. Thus, this study explored the protective effect of virtuous personality trait on adolescents’ online deviant behavior and its psychological mechanism. A total of 851 Chinese college students anonymously completed a series of questionnaires about virtuous personality trait, moral disengagement, perspective taking, and online deviant behavior. The findings showed the following: (1) Virtuous personality trait was negatively correlated with online deviant behavior. (2) Moral disengagement mediated the relationship between virtuous personality trait and online deviant behavior. (3) Perspective taking moderated the first half stage of the mediation model in which college students’ virtuous personality trait influences online deviant behavior via moral disengagement. (4) A moderated moderated-mediation analysis found that gender moderated the moderating effect of perspective taking on the relationship between virtuous personality trait and moral disengagement. This study is helpful to demonstrate the protective effect and psychological mechanism of virtuous personality trait on online deviant behavior. Some theoretical and practical significance and limitations were also analyzed and discussed.
Effects of Tribulus terrestris L. on Sport and Health Biomarkers in Physically Active Adult Males: A Systematic Review
Authors: Fernández-Lázaro Diego , Fernandez-Lazaro Cesar , Seco-Calvo Jesús , Garrosa Evelina , Adams David , Mielgo-Ayuso Juan
Published: August 2022

Tribulus terrestris L. (TT) is a plant used in traditional Chinese medicine, Ayurvedic medicine, and sports nutrition to improve health and performance. However, no conclusive evidence exists about the potential beneficial effects of TT on sport and health biomarkers in physically active adults. Based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and the modified McMaster Critical Review Form for methodological quality assessment, we systematically reviewed studies indexed in Web of Science, Scopus, and PubMed, to assess the effects of TT on immunological, hematological, biochemical, renal, lipidic, hormonal behavior, and anti-inflammatory response in physically active adult males. Among 340 records identified in the search, a total of 7 studies met the inclusion and exclusion criteria. Overall, participants supplemented with TT displayed significant improvements in lipid profile. Inflammatory and hematological biomarkers showed moderate beneficial effects with no significant changes on renal biomarkers. No positive effects were observed on the immune system response. Additionally, no TT-induced toxicity was reported. In conclusion, there was no clear evidence of the beneficial effects of TT supplementation on muscle damage markers and hormonal behavior. More studies are needed to confirm the benefits of TT due to the limited number of studies available in the current literature.
Retrospective Analysis of the Factors Affecting the Induction of Childbirth in 4350 Women from a Single Center in Warsaw, Poland
Authors: Bączek Grażyna , Rzońca Ewa , Rzońca Patryk , Rychlewicz Sylwia , Budner Margareta , Bień Agnieszka
Published: August 2022

Labor induction is one of the most common procedures performed during childbirth, on average in 20–30% of all pregnant women. The aim of this paper was to perform a retrospective analysis of the factors influencing the induction of childbirth. The data provide population-based evidence for Poland (Masovian Voivodeship). The electronic patient records of a hospital in Warsaw were used to create an anonymous retrospective database of all deliveries from 2015 to 2020. The study included an analysis of two groups of patients. The study group consisted of patients with labor induction—4350 cases, and the control group of patients with spontaneous contractions—20,345. The factors influencing the lower frequency of labor induction in the study group were previous cesarean section (OR = 0.73, 95% CI: 0.64–0.84, p < 0.05) and a higher number of deliveries (OR = 0.74, 95% CI: 0.68–0.80, p < 0.05). It is necessary to conduct further research about obstetric procedures used during childbirth, such as induction of childbirth, to reduce the risk of complications and improve the perinatal care of the mother and the neonate.
Behavioral Economics in the Epidemiology of the COVID-19 Pandemic: Theory and Simulations
Authors: Marin-Lopez Blas A. , Jimenez-Gomez David , Abellán-Perpiñán José-María
Published: August 2022

We provide a game-theoretical epidemiological model for the COVID-19 pandemic that takes into account that: (1) asymptomatic individuals can be contagious, (2) contagion is behavior-dependent, (3) behavior is determined by a game that depends on beliefs and social interactions, (4) there can be systematic biases in the perceptions and beliefs about the pandemic. We incorporate lockdown decisions by the government into the model. The citizens’ and government’s beliefs can exhibit several biases that we discuss from the point of view of behavioral economics. We provide simulations to understand the effect of lockdown decisions and the possibility of “nudging” citizens in the right direction by improving the accuracy of their beliefs.
Sustainable Population Size at the County Level under Limited Development Policy Constraints: Case Study of the Xihaigu Mountain Area, Northwest China
Authors: Chen Xueli , Song Yongyong , Fan Xingang , Ma Jing
Published: August 2022

Understanding the extent to which demographic changes may affect the prospects of sustainable development is a priority for both academics and policy makers. Accordingly, we attempted to explore the population growth limit of the Xihaigu Mountain area in China. To analyze the optimum population at the county level, the relationship curve between population size and environmental quality was fitted using panel data (2009–2018). The sustainable population size of each county was determined by integrating the population carrying capacity of land resources and optimum population. The results show that the relationship between regional population size and environmental quality conforms to an inverted N-shaped curve. To maintain sustainable development, the population size of Tongxin, Xiji, and Haiyuan should be in the range of 320,800–379,800, 315,800–416,900, and 333,500–416,900, respectively. The current population size of other counties should be maintained, and their surplus construction lands are acceptable to be used for environmentally friendly industries rather than population expansion. We demonstrated a practical approach to calculate a dynamic range of population size under the dual constraints of resource and environment, which overcomes the shortcoming of only considering the maximum carrying capacity to a limited extent. We also identified the population boundary in a “steady-state economy” and quantified planetary boundaries of population in Xihaigu Mountain area using a dynamic sustainable population size. The findings provide decision-making references for the local government.
Research Progress of Music Therapy on Gait Intervention in Patients with Parkinson’s Disease
Authors: Wu Zhuolin , Kong Lingyu , Zhang Qiuxia
Published: August 2022

Music therapy is an effective way to treat the gait disorders caused by Parkinson’s disease. Rhythm music stimulation, therapeutic singing, and therapeutic instrument performance are often used in clinical practice. The mechanisms of music therapy on the gait of patients with Parkinson’s disease include the compensation mechanism of cerebellum recruitment, rhythm entrainment, acceleration of motor learning, stimulation of neural coherence, and increase of cortical activity. All mechanisms work together to complete the intervention of music therapy on patients’ gait and help patients to recover better. In this paper, the effect of music therapy on gait disorders in Parkinson’s disease patients was reviewed, and some suggestions were put forward.
The Genomic 3′ UTR of Flaviviruses Is a Translation Initiation Enhancer
Authors: Berzal-Herranz Alfredo , Berzal-Herranz Beatriz , Ramos-Lorente Sara Esther , Romero-López Cristina
Published: August 2022

Viruses rely on the cellular machinery of host cells to synthesize their proteins, and have developed different mechanisms enabling them to compete with cellular mRNAs for access to it. The genus Flavivirus is a large group of positive, single-stranded RNA viruses that includes several important human pathogens, such as West Nile, Dengue and Zika virus. The genome of flaviviruses bears a type 1 cap structure at its 5′ end, needed for the main translation initiation mechanism. Several members of the genus also use a cap-independent translation mechanism. The present work provides evidence that the WNV 5′ end also promotes a cap-independent translation initiation mechanism in mammalian and insect cells, reinforcing the hypothesis that this might be a general strategy of flaviviruses. In agreement with previous reports, we show that this mechanism depends on the presence of the viral genomic 3′ UTR. The results also show that the 3′ UTR of the WNV genome enhances translation of the cap-dependent mechanism. Interestingly, WNV 3′ UTR can be replaced by the 3′ UTR of other flaviviruses and the translation enhancing effect is maintained, suggesting a molecular mechanism that does not involve direct RNA-RNA interactions to be at work. In addition, the deletion of specific structural elements of the WNV 3′ UTR leads to increased cap-dependent and cap-independent translation. These findings suggest the 3′ UTR to be involved in a fine-tuned translation regulation mechanism.
Cloning of the Maternal Effector Gene org and Its Regulation by lncRNA ORG-AS in Chinese Tongue Sole (Cynoglossus semilaevis)
Authors: Zhao Xiaona , Feng Bo , Wang Qian , Tang Lili , Liu Qian , Ma Wenxiu , Li Chenghua , Shao Changwei
Published: August 2022

Maternal effector genes (MEGs) encode maternal RNA and protein, accumulating in the cytoplasm of oocytes. During oocyte development, MEGs participate in oocyte meiosis and promote oocyte development. And MEGs can also regulate maternal transcriptome stability and promote maternal–zygotic transition (MTZ) in early embryonic development. Long noncoding RNAs (lncRNAs), as new epigenetic regulators, can regulate gene expression at both the transcriptional and post-transcriptional levels through cis- or trans-regulation. The oogenesis-related gene org is a germ-cell-specific gene in fish, but the role of org in embryonic development and oogenesis has rarely been studied, and the knowledge of the lncRNA-mediated regulation of org is limited. In this study, we cloned and identified the org gene of Chinese tongue sole (Cynoglossus semilaevis), and we identified a lncRNA named lncRNA ORG-anti-sequence (ORG-AS), located at the reverse overlapping region of org. The results of qRT-PCR and FISH demonstrated that org was highly expressed during the early stages of embryonic development and oogenesis and was located in the cytoplasm of oocytes. ORG-AS was expressed at low levels in the ovary and colocalized with org in the cytoplasm of oocytes. In vitro experiments showed that overexpression of ORG-AS inhibited org expression. These results suggest that org, as a MEG in C. semilaevis, participates in the MTZ and the oogenesis. The lncRNA ORG-AS negatively regulates the gene expression of org through trans-regulation. These new findings broaden the function of MEGs in embryonic development and the oogenesis of bony fish and prove that lncRNAs are important molecular factors regulating org.
Molecular Mechanisms of Cytotoxicity of NCX4040, the Non-Steroidal Anti-Inflammatory NO-Donor, in Human Ovarian Cancer Cells
Authors: Sinha Birandra K. , Tokar Erik J. , Bortner Carl D.
Published: August 2022

NCX4040, the non-steroidal anti-inflammatory-NO donor, is cytotoxic to several human tumors, including ovarian tumor cells. We have found that NCX4040 is also cytotoxic against both OVCAR-8 and its adriamycin resistant (NCI/ADR-RES) tumor cell lines. Here, we have examined mechanism(s) for the cytotoxicity of NCX4040 in OVCAR-8 and NCI/ADR-RES cell lines. We found that NCX4040 induced significant apoptosis in both cell lines. Furthermore, NCX4040 treatment caused significant depletion of cellular glutathione, causing oxidative stress due to the formation of reactive oxygen/nitrogen species (ROS/RNS). Significantly more ROS/RNS were detected in OVCAR-8 cells than in NCI/ADR-RES cells which may have resulted from increased activities of SOD, glutathione peroxidase and transferases expressed in NCI/ADR-RES cells. NCX4040 treatment resulted in the formation of double-strand DNA breaks in both cells; however, more of these DNA breaks were detected in OVCAR-8 cells. RT-PCR studies indicated that NCX4040-induced DNA damage was not repaired as efficiently in NCI/ADR-RES cells as in OVCAR-8 cells which may lead to a differential cell death. Pretreatment of OVCAR-8 cells with N-acetylcysteine (NAC) significantly decreased cytotoxicity of NCX4040 in OVCAR-8 cells; however, NAC had no effects on NCX4040 cytotoxicity in NCI/ADR-RES cells. In contrast, FeTPPS, a peroxynitrite scavenger, completely blocked NCX4040-induced cell death in both cells, suggesting that NCX4040-induced cell death could be mediated by peroxynitrite formed from NCX4040 following cellular metabolism.
High-Efficiency and High-Quality Extraction of Hemicellulose of Bamboo by Freeze-Thaw Assisted Two-Step Alkali Treatment
Authors: Wang Xin , He Jiahao , Pang Shuyu , Yao Shuangquan , Zhu Chunxia , Zhao Jinwei , Liu Yang , Liang Chen , Qin Chengrong
Published: August 2022

Hemicellulose is a major component of the complex biomass recalcitrance structure of fiber cell walls. Even though biomass recalcitrance protects plants, it affects the effective utilization of lignocellulosic biomass resources. Therefore, the separation and extraction of hemicellulose is very important. In this study, an improved two-step alkali pretreatment method was proposed to separate hemicellulose efficiently. Firstly, 16.61% hemicellulose was extracted from bamboo by the weak alkali treatment. Then, the physical freezing and the alkali treatment were carried out by freezing at −20 °C for 12.0 h and thawing at room temperature, heating to 80 °C, and treating with 5.0% sodium hydroxide for 90 min; the extraction yield of hemicellulose reached 73.93%. The total extraction yield of the two steps was 90.54%, and the molecular weight and purity reached 44,865 g·mol–1 and 89.60%, respectively. It provides a new method for breaking the biomass recalcitrance of wood fiber resources and effectively extracting hemicellulose.
High Housing Density-Induced Chronic Stress Diminishes Ovarian Reserve via Granulosa Cell Apoptosis by Angiotensin II Overexpression in Mice
Authors: Kim Jihyun , You Sooseong
Published: August 2022

Repeated and prolonged stress causes hypothalamic-pituitary-adrenal (HPA) dysregulation. Excessive hypothalamic-pituitary-adrenal axis activity has been linked to inadequate activation of the hypothalamus-pituitary-ovarian axis, which controls the growth and development of ovarian follicles and oocytes. Therefore, we assessed the ovarian reserve under high-housing-density-induced prolonged stress, and investigated the mechanisms underlying diminished ovarian reserve in this study. Eight-week-old female C57BL/6 mice were housed for 10 weeks under different housing densities. We then assessed hormone levels, performed histology and immunohistochemistry analyses of ovarian follicles, evaluated ovarian mRNA expression, and measured angiotensin II-mediated apoptosis in vitro. More densely housed mice presented increased corticosterone levels and decreased follicle-stimulating and luteinizing hormone levels. Moreover, mice exposed to prolonged ordinary stress showed a reduced level of serum anti-Müllerian hormone and an increased number of atretic ovarian follicles. Stressed mice showed increased levels of angiotensinogen and angiotensin II in the ovaries and serum. Furthermore, our in vitro study confirmed that high-housing-density-related stress induced granulosa cell apoptosis, resulting in diminished ovarian reserves. Collectively, our findings highlight the importance of women managing everyday stress to maintain their reproductive health.
Screening and Verification of Photosynthesis and Chloroplast-Related Genes in Mulberry by Comparative RNA-Seq and Virus-Induced Gene Silencing
Authors: Li Yong , Yu Cui , Mo Rongli , Zhu Zhixian , Dong Zhaoxia , Hu Xingming , Deng Wen , Zhuang Chuxiong
Published: August 2022

Photosynthesis is one of the most important factors in mulberry growth and production. To study the photosynthetic regulatory network of mulberry we sequenced the transcriptomes of two high-yielding (E1 and E2) and one low-yielding (H32) mulberry genotypes at two-time points (10:00 and 12:00). Re-annotation of the mulberry genome based on the transcriptome sequencing data identified 22,664 high-quality protein-coding genes with a BUSCO-assessed completeness of 93.4%. A total of 6587 differentially expressed genes (DEGs) were obtained in the transcriptome analysis. Functional annotation and enrichment revealed 142 out of 6587 genes involved in the photosynthetic pathway and chloroplast development. Moreover, 3 out of 142 genes were further examined using the VIGS technique; the leaves of MaCLA1- and MaTHIC-silenced plants were markedly yellowed or even white, and the leaves of MaPKP2-silenced plants showed a wrinkled appearance. The expression levels of the ensiled plants were reduced, and the levels of chlorophyll b and total chlorophyll were lower than those of the control plants. Co-expression analysis showed that MaCLA1 was co-expressed with CHUP1 and YSL3; MaTHIC was co-expressed with MaHSP70, MaFLN1, and MaEMB2794; MaPKP2 was mainly co-expressed with GH9B7, GH3.1, and EDA9. Protein interaction network prediction revealed that MaCLA1 was associated with RPE, TRA2, GPS1, and DXR proteins; MaTHIC was associated with TH1, PUR5, BIO2, and THI1; MaPKP2 was associated with ENOC, LOS2, and PGI1. This study offers a useful resource for further investigation of the molecular mechanisms involved in mulberry photosynthesis and preliminary insight into the regulatory network of photosynthesis.