bluuebunny/crossref_metadata_2025 huggingface dataset:
Decadal Changes in Bomb-Produced Radiocarbon in the Pacific Ocean from the 1990s to 2000s
Authors: Kumamoto Yuichiro , Murata Akihiko , Kawano Takeshi , Watanabe Shuichi , Fukasawa Masao
Published: January 2017

In the 2000s, radiocarbon in dissolved inorganic carbon was measured during 7 revisit cruises along the lines of the World Ocean Circulation Experiment in the Pacific Ocean. Comparison of 14C data along these lines from the 1990s and 2000s revealed decadal changes of 14C concentration in the thermocline, most of which were due to temporal changes in the bomb-produced 14C. Vertical profiles and vertical-integrated inventories of the bomb 14C in the subarctic and equatorial regions did not change appreciably. In the southern subtropical region, 14C decreased in the upper thermocline from the surface to ∼500 m depth. In contrast, 14C increased in the lower thermocline below ∼500 m depth. The opposing directions in 14C change resulted in small temporal changes in the total inventory of bomb 14C. On the other hand, the water-column inventory significantly decreased in the northwestern subtropical region due to the 14C decrease in the upper thermocline. These decadal changes in bomb 14C indicate that the turnover time of thermocline circulation in the northwestern subtropical region is faster than that in the southern subtropical region, and imply an interbasin transport of bomb 14C from the North Pacific to other basins.
Quantification of Sedimentary Organic Carbon Storage and Turnover of Tidal Mangrove Stands in Southern China Based on Carbon Isotopic Measurements
Authors: Zhang J P , Yi W X , Shen C D , Ding P , Ding X F , Fu D P , Liu K X
Published: January 2017

Mangrove ecosystems are highly productive and play an important role in tropical and global coastal carbon (C) budgets. However, sedimentary organic carbon (SOC) storage and turnover in mangrove forests are still poorly understood. Based on C isotopic measurements of sediment cores of 2 mangrove stands in southern China, SOC density was 431.77 Mg ha−1at site 1 (aAegiceras corniculatum-dominated high tidal stand) and 243.65 Mg ha−1in site 2 (aBruguiera gymnorrhiza+Kandelia candel-dominated middle tidal stand). SOC δ13C values at both mangrove sites ranged from -29.4% to −26.0%. SOC δ13C was enriched with depth at 20–50 cm at site 1, which possibly resulted from preferential microbial decomposition. SOC δ13C at site 2 experienced frequent tidal flushing, and presented relatively stable values with depth. A bomb-14C-based SOC turnover model indicated that turnover times of SOC at 20–50 cm at site 1 were 4.44–26.04 yr. Modern C input from abundant roots might account for the very short SOC turnover times at these subsurface layers. As a result, our study suggested that tidal processes had a great influence on SOC storage and turnover in mangrove forests.
Radiocarbon Age-Depth Modeling Prevents Misinterpretation of Past Vegetation Dynamics: Case Study of Wierchomla Mire (Polish Outer Carpathians)
Authors: Michczyński Adam , Kołaczek Piotr , Margielewski Włodzimierz , Michczyńska Danuta J , Obidowicz Andrzej
Published: January 2017

An age-depth model based on radiocarbon dates was produced from a Holocene profile collected from a rich fen situated in the Beskid Sądecki Mountains (the Outer Western Carpathians, southern Poland). The model is compared against the results of palynological and loss on ignition (LOI) analyses supplemented by the identification of organic deposits. Five distinct palynological episodes are detected. These potential palynological age markers are critically compared with the results of age-depth modeling and other dated profiles. The results presented distinctly show that using palynological episodes as age markers for age-depth construction may be highly misleading.
Present-Day Radiocarbon Content of Select Flavoring Compounds Reveals Vanillin Production Pathway
Authors: Culp Randy , Prasad G V Ravi
Published: January 2017

Radiocarbon and stable isotope determination in foods, flavors, and beverages, for the authentication of source material and process of formation, is a well-established method of identity used in industry. New methods of provenance determination, using stable isotopes of oxygen and hydrogen, have added to the host of other isotopic methods used for characterizing natural or botanically derived products. The unambiguous determinant of a product's fossil fuel origin be it from petroleum, natural gas, or coal, is through the measurement of its 14C content. The 14C content can also be used to determine the fraction dilution of recently grown and harvested material with that derived from fossil fuel, and even confirms the vintage of agricultural products based on the well-established decrease of bomb-produced atmospheric 14C. This paper documents 14C measurements at the University of Georgia's Center for Applied Isotope Studies accelerator mass spectrometry and stable isotope laboratories, over the last 3 yr, for 10 important flavoring compounds. By establishing an accurate and current level of 14C in botanically derived products, we were able to confirm a particular source for vanilla production, the most popular consumer flavor in the marketplace. Over the years, vanilla extract has been produced less and less from vanilla beans (Vanilla planifolia), particularly those from Madagascar and the Comoros Islands, and more from other botanical precursors such as ferulic acid, clove oil, and guaiacol. We report isotopic data to support this precursor for vanilla production based on high 14C levels accumulated during the tree's life, incorporated in the tree rings and their associated stable isotope abundances.
Measuring 14C Concentration in Wine to Monitor Global Distribution of 14C
Authors: Sakurai Hirohisa , Namai Saori , Inui Emiko , Tokanai Fuyuki , Kato Kazuhiro , Takahashi Yui , Sato Taichi , Kikuchi Satoshi , Arai Yumi , Masuda Kimiaki , Shibata Katsumasa , Kuriyama Yasunao
Published: January 2017

Using liquid scintillation counting (LSC) and accelerator mass spectrometry (AMS), radiocarbon concentrations were measured for wine from 8 wineries located in 7 countries in the Northern and Southern hemispheres. The 14C concentrations of ethanol and residual materials in the wine were correlated (correlation coefficient 0.82). The δ14C measurements of wine samples from the mid-latitudes in the Northern Hemisphere were approximately 1l% lower than the extrapolations from Schauinsland data, suggesting a local fossil fuel effect. δ14C measurements from the wine samples from the Southern Hemisphere were higher than those from the Northern Hemisphere. The offsets of the 4 wine δ14C measurements were significant, with values between approximately 8% and 15%. Because the harvest years of the mixed grapes were estimated to be 7–12 yr older than their vintage years, this leads to a caveat when determining the 14C concentrations of the year using the wine vintage.
Determining 14C Content in Different Human Tissues: Implications for Application of 14C Bomb-Spike Dating in Forensic Medicine
Authors: Calcagnile Lucio , Quarta Gianluca , Cattaneo Cristina , D'Elia Marisa
Published: January 2017

Various samples extracted from human tissues (with different radiocarbon turnover rates) of a post-bomb human body were submitted to accelerator mass spectrometry (AMS) 14C dating: hair; a cortical fraction of a skull bone; a trabecular fraction of a pubic symphysis; and enamel extracted from permanent teeth with different dates of formation were analyzed. The analyzed samples showed varying 14C concentrations corresponding to different times of formation or different turnover rates. The implications of the results in forensics studies are discussed.
Radiocarbon Dating of Fugendake Volcano in Unzen, SW Japan
Authors: Xu Sheng , Hoshizumi Hideo , Uto Kozo , Freeman Stewart P H T
Published: January 2017

This article presents new radiocarbon ages for the lavas, pyroclastic flow, and lahar deposits that originated from the Fugendake and Mayuyama volcanoes of the Younger Unzen Volcano, SW Japan. Nine charcoal samples were collected from the lavas and pyroclastic flow deposits, and 17 soil samples from the underlying volcanic-related products. This data set, together with previously published ages (thermoluminescence, K-Ar, fission track, and 14C), yielded new information about the timing of Late Pleistocene eruptions and an improved understanding of the evolution of the Fugendake and Mayuyama volcanoes. Fugendake Volcano started to build within the scar of Myokendake around 29 cal ka BP, and its eruption products spread over the flank of Myokendake. The remarkable eruptions of Fugendake Volcano included the lava and pyroclastic flow deposits around 22, 17, 12, and 4.5 cal ka BP. Subsequent historical eruptions occurred in AD 1663, 1792, and 1991–1995. Developed on the eastern extension of Fugendake Volcano, Mayuyama Volcano was active during the building stage of Fugendake at 4.5 cal ka BP. This study also identified a pumice eruption at ∼10 ka and 2 volcanic-related lahar deposits around 1.6 and 0.7 ka, which need to be addressed in future research.
Optical Detection of Radiocarbon Dioxide: First Results and AMS Intercomparison
Authors: Galli I , Bartalini S , Cancio P , De Natale P , Mazzotti D , Giusfredi G , Fedi M E , Mandò P A
Published: January 2017

The first results of an optical method for measuring radiocarbon concentrations, based on mid-infrared laser spectroscopy of a carbon dioxide gas sample, are presented with the theoretical bases explained in detail. The first measurements on modern and highly enriched samples show the extreme linearity of this technique over more than 5 decades. An intercomparison with accelerator mass spectrometry (AMS) is performed both for modern and 14C-dead samples, assessing the almost perfect agreement of their respectively measured concentration values. The main features of our technique are compared with liquid scintillation counting (LSC) and AMS, and future developments of the current setup are discussed.
Present Status of YU-AMS System
Authors: Tokanai Fuyuki , Kato Kazuhiro , Anshita Minoru , Sakurai Hirohisa , Izumi Akihiro , Toyoguchi Teiko , Kobayashi Takeshi , Miyahara Hiroko , Ohyama Motonari , Hoshino Yasuharu
Published: January 2017

A new compact accelerator mass spectrometry (AMS) system has been installed in the Kaminoyama Research Institute at Yamagata University (YU). The AMS system is based on a 0.5MV Pelletron accelerator developed by the National Electrostatics Corporation. An automated acid-alkali-acid (AAA) treatment system and an automated graphitization line were also installed in the same facility for sample preparation. Performance tests of the YU-AMS system were carried out by measuring the C-series standard samples (C1–C8) and HOxII provided by IAEA and NIST, respectively. We evaluated the YU-AMS system by comparing the radiocarbon ages of Japanese tree rings with dendrochronologically determined calendar ages with calibration data. We also carried out some performance tests using a control serum and a 14C-labeled drug (oxaliplatin).
Upgrade of the CO2 Direct Absorption Method for Low-Level 14C Liquid Scintillation Counting
Authors: Canducci Chiara , Bartolomei Paolo , Magnani Giuseppe , Rizzo Antonietta , Piccoli Angela , Tositti Laura , Esposito Massimo
Published: January 2017

A new system for CO2 absorption and liquid scintillation counting (LSC) was designed and developed along with its inherent measurement protocol for radiocarbon analysis in gaseous emissions, fuels, and biobased products. CO2 is chemically trapped as a carbamate in a suitable absorbing solution (3-methoxy-propyl-amine), gravimetrically measured, and analyzed by LSC (using a QuantulusTM 1220) to determine the 14C content. The use of cryogenic traps and a pressure transducer in the system prevents the need for closed-loop recirculation or additional steps to maximize CO2 capture in a short amount of time. The choice of PTFE vials used both for CO2 pretreatment and subsequent LSC analysis provides the opportunity to significantly reduce the background counting down to 40% with respect to the low-40K glass vials. This upgrade resulted in improving the maximum detectable age back to 36,000 yr BP in routine measurements. This method therefore turns out to be flexible enough to be applied for 14C dating as well as to differentiate between modern and fossil carbon.
EnvironMICADAS: A Mini 14C AMS with Enhanced Gas Ion Source Interface in the Hertelendi Laboratory of Environmental Studies (HEKAL), Hungary
Authors: Molnár M , Rinyu L , Veres M , Seiler M , Wacker L , Synal H-A
Published: January 2017

A more developed and modern technology replaced the old radiocarbon measuring methods in Hungary, based on isotope separation by accelerator mass spectrometry (AMS) and not on activity measurements. In summer 2011, the EnvironMICADAS was successfully installed in the Laboratory of HEKAL, Debrecen, Hungary. In this project, a multipurpose gas-handling system was developed for the gas ion source of EnvironMICADAS at ETH Zürich, designed for the measurement of small environmental origin samples (<50 μg carbon) with moderate precision requirements. The ultimate aim is an automated device for high sample throughput. Since its final installation and first year of operation, over 2000 graphite targets were analyzed. Long-term stability of the instrument is confirmed through measurements of the Ox-II standard and processed blank targets during the first half of 2012.
Radiocarbon Dating of Mummified Human Remains: Application to a Series of Coptic Mummies from the Louvre Museum
Authors: Richardin Pascale , Coudert Magali , Gandolfo Nathalie , Vincent Julien
Published: January 2017

Many French museums keep in their reserves a great number of mummified human remains. Beyond any ethical or deontological issues, they constitute an important part of our archaeological and historical heritage. Their dating is often inexact and imprecise, but nevertheless this parameter is very interesting, especially if correlated or associated with other analytical or typological data, e.g. the process of mummification. The present study has been carried out in the context of a multidisciplinary scientific program on a set of Coptic mummies found at the site of Antinoe (Egypt), deposited in the Louvre Museum or sent by the state to various other French museums. To minimize the sample size, we have developed a new method for the pretreatment of hair samples before accelerator mass spectrometry (AMS) radiocarbon dating. Thus, we have taken samples from ≃30 mummies, distributed in 8 museums around France, and also from different textiles or plants near or on the bodies. The results and conclusions show the importance of dating mummies in a museum context. For example, 14C dates permit the exclusion of the assumed relationship of a woman with a child. Similarly, a hair sample from the head of a mummy presents a very different 14C date from that of the hair across her chest. The results show that these hairs came from another mummy and were probably placed there intentionally by the people in charge of the collections.
Intercomparison of 14C Dating of Wood Samples at Lund University and ETH-Zurich AMS Facilities: Extraction, Graphitization, and Measurement
Authors: Adolphi F , Güttler D , Wacker L , Skog G , Muscheler R
Published: January 2017

We conducted an interlaboratory comparison between our radiocarbon-related research group at Lund University and the established ETH-Zurich facility to test the quality of the results obtained in Lund and to identify sources of potential background differences and scatter. We did find differences between the 2 laboratories in the contributions of chemical preparation, graphitization, and measurements to the overall background. The resulting overall background is, however, almost similar. Multiple measurements on 2 wood samples of known calendar age yield consistent and accurate 14C ages in both laboratories. However, one of our known samples indicates that IntCal09 is ≃38 ± 16 14C BP too young at 7020 calendar yr BP, which is consistent with one of the raw data sets contributing to IntCal09. Overall, our results show that a systematic approach to compare the different steps involved in 14C age determination is a useful exercise to pinpoint targets for improvement of lab routines and assess interlaboratory differences. These effects do not necessarily become apparent when comparing 14C measurements that integrate over the whole process of preparation and measurement of different laboratories.
14C Mortar Dating: The Case of the Medieval Shayzar Citadel, Syria
Authors: Nonni Sara , Marzaioli Fabio , Secco Michele , Passariello Isabella , Capano Manuela , Lubritto Carmine , Mignardi Silvano , Tonghini Cristina , Terrasi Filippo
Published: January 2017

This paper reports the results from applying the Cryo2SoniC (Cryobreaking, Sonication, Centrifugation) protocol to some lime mortars sampled from the citadel of Shayzar (Syria). The overall aims of this project are 1) to use the properties offered by high-precision accelerator mass spectrometry (AMS) radiocarbon dating for the evaluation of absolute chronology with its typical robust time constraints (i.e. 25 14C yr), and 2) to apply the dating directly to the citadel structures in order to prevent possible biasing effects potentially affecting indirect 14C dating on organic materials found at the study site. The analyses presented in this paper have been mainly performed as a preliminary check of the Cryo2SoniC methodology in order to assess its applicability to this study site by comparing observed mortar results with archaeological expectations about the citadel development phasing and charcoals found encased in mortars. Petrographic and mineralogical thin-section analyses by optical microscopy (TSOM), X-ray powder diffraction (XRD), and scanning electron microscopy plus energy dispersive spectroscopy (SEM/EDS) investigations were carried out for characterization of the mortar samples to verify the occurrence of some features, related to their production technology, which may introduce dating offsets. The resulting 14C calibrated ages were in agreement with the archaeological expectations based on type and stratigraphic site reconstructions, in situ inscriptions, and written sources. Such results showed also a general (with 1 exception) statistical agreement among the charcoals and the analyzed mortars simultaneously, confirming the archaeological expectations for the Shayzar citadel. Results presented in this paper indicate good accuracy for the applied procedure for chronology reconstruction and highlight the capability of Cryo2SoniC to further characterize the Shayzar site.
Simple, Rapid, and Cost Effective: A Screening Method for 14C Analysis of Small Carbonate Samples
Authors: Bush Shari L , Santos Guaciara M , Xu Xiaomei , Southon John R , Thiagarajan Nivedita , Hines Sophia K , Adkins Jess F
Published: January 2017

We have developed a simple, rapid method to screen carbonates for survey applications, which provides radiocarbon dates with decreased precision at lower cost. The method is based on previous work by Longworth et al. (2011) and involves mixing pulverized CaCO3 with Fe powder, followed by pressing into aluminum target holders for direct 14C accelerator mass spectrometry (AMS) measurements. An optimum beam current averaging ∼10% of those produced by >0.7 mg C graphite targets was obtained for carbonate samples of 0.3–0.5 mg (0.04–0.06 mg C). The precision of the method was evaluated by measuring triplicates of 14C reference materials, as well as by comparing results from this rapid method with results from high-precision AMS measurements on graphite (typically 0.2–0.3%). Measurement reproducibility was ∼1.8% (1σ) for samples <10 ka BP, and it increased drastically for older samples. However, t tests on paired samples resulted in p values greater than 0.05, indicating a good correlation between this survey method and the conventional one. An average blank (calcite) of 0.0075 Fm (∼39 ka BP) was achieved. The simplicity of the technique allowed us to process and measure 72 deep-sea coral samples in less than 25 hr.
Compound-Specific Radiocarbon Dating of Essential and Non-Essential Amino Acids: Towards Determination of Dietary Reservoir Effects in Humans
Authors: Nalawade-Chavan Shweta , McCullagh James , Hedges Robert , Bonsall Clive , Boroneanţ Adina , Ramsey Christopher Bronk , Higham Thomas
Published: January 2017

When humans consume foods from different radiocarbon reservoirs offset in age to the atmosphere, inaccuracies in the 14C date of bone collagen can occur. Mesolithic human skeletons from the Iron Gates section of the Lower Danube Valley have yielded reservoir offsets of up to ∼500 yr. This has been demonstrated through direct dating of bulk collagen from human bones and the remains of ungulate bone projectile points that were found embedded in them (Cook et al. 2001). We present improvements to a novel HPLC method for the detection and separation of underivatized amino acids using a water-only mobile phase free of organic or inorganic modifiers, ensuring very low carbon backgrounds. Our hypothesis is that direct 14C dating of single essential and non-essential amino acids might allow an improvement in the dating accuracy for reservoir-affected human bones. The method facilitates separation of less polar amino acids (mostly “essential”), currently not possible in the recently published protocol. We discuss methodological developments, demonstrate carbon backgrounds, and present analytical approaches to minimize their effects. We validate the precision and accuracy of the method by accelerator mass spectrometry (AMS) dating relatively modern and 14C-dead, known-age bone standards. Finally, we apply the method to the dating of single amino acids from bone samples with a proven ∼500–yr carbon reservoir effect from Mesolithic burials at the Iron Gates sites. We investigate whether differences can be found in AMS dates for essential and non-essential amino acids since, although contemporaneous, these are expected to derive from dietary sources with differing 14C reservoirs.
Recent and Planned Developments of the Program OxCal
Authors: Ramsey Christopher Bronk , Lee Sharen
Published: January 2017

OxCal is a widely used software package for the calibration of radiocarbon dates and the statistical analysis of 14C and other chronological information. The program aims to make statistical methods easily available to researchers and students working in a range of different disciplines. This paper will look at the recent and planned developments of the package. The recent additions to the statistical methods are primarily aimed at providing more robust models, in particular through model averaging for deposition models and through different multiphase models. The paper will look at how these new models have been implemented and explore the implications for researchers who might benefit from their use. In addition, a new approach to the evaluation of marine reservoir offsets will be presented. As the quantity and complexity of chronological data increase, it is also important to have efficient methods for the visualization of such extensive data sets and methods for the presentation of spatial and geographical data embedded within planned future versions of OxCal will also be discussed.
Biomass Growth Rate of Trees from Cameroon Based on14C Analysis and Growth Models
Authors: Tandoh J B , Marzaioli F , Battipaglia G , Capano M , Castaldi S , Lasserre B , Marchetti M , Passariello I , Terrasi F , Valentini R
Published: January 2017

The question of whether the rise in CO2levels observed during the industrial era has influenced the rates of tree biomass growth represents one of the main unsolved questions in the field of climate change science. In this framework, the African tropical forest represents one of the most important carbon (C) sinks, but detailed knowledge of its response to elevated CO2is still lacking, especially regarding tree growth rate estimations. A major limitation to determining growth rates in the African tropical region is that many trees lack seasonality in cambial activity determining annual growth rings. In this study, several species of trees characterizing the African tropical forest have been investigated to estimate their biomass growth rate by means of a procedure based on14C and growth models. A total of 71 subsamples were analyzed for aEntandrophragma cylindricum(sapele) tree, and 38 and 25 wood subsamples forErythrophleum suaveolens(tali) andTriplochiton scleroxylon(ayous) trees, respectively, using radiocarbon measurements at the Centre for Isotopic Research on Cultural and Environmental Heritage (CIRCE). All measured modern samples were in agreement with the Southern Hemisphere (SH)14C bomb-spike curve. Observed preliminary results indicate a decrease in the growth rate of the sapele tree (∼350 yr old) in the industrial period compared to the pre-industrial era. Growth rates for trees of the other 2 species were higher than sapele, with ayous being the fastest-growing species.
Variations in Soil CO2 Concentrations and Isotopic Values in a Semi-Arid Region Due to Biotic and Abiotic Processes in the Unsaturated Zone
Authors: Carmi I , Yakir D , Yechieli Y , Kronfeld J , Stiller M
Published: January 2017

A study of CO2 in soil gas was conducted in a bare plot in the unsaturated zone (USZ) of Yatir Forest, northern Negev, Israel. In 2006, 6 tubes for sampling of soil gas were inserted into the USZ to depths of 30, 60, 90, 120, 200, and 240 cm. Profiles of soil gas in the USZ were collected from the tubes 5 times between October 2007 and September 2008. Measurements of the collected profiles of soil gas were of CO2 (ppm), δ13C (′), and Δ14C (′). At all times, the concentration of CO2 in the soil gas was higher than in the air at the surface (CO2 ≃ 400 ppm; δ13C ≃ −9′). The main source of the CO2 in soil gas is from biotic activity released through roots of trees and of seasonal plants close to the surface. In the winter, the CO2 concentrations were lowest (6000 ppm) and the δ13C was −20′. In the spring and through the summer, the CO2 concentration increased. It was estimated that the major source of CO2 is at ≃240 cm depth (δ13C ≃ −22′; CO2 ≃ 9000 ppm) or below. Above this level, the concentrations decrease and the δ13C (′) become more positive. The 14C values in the measured profile are all less than atmospheric and biotic 14C. It was deduced that biotic CO2 dissolves in porewater to form carbonic acid, which then dissolves secondary carbonate (δ13C ≃ −8′; 14C ≃ −900′) from the sediments of the USZ. With the 14C data, the subsequent release of CO2 into the soil gas was then estimated. The 14C data, supported by the 13C and CO2 data, also indicate a biotic source at the root zone, at about 90 cm depth.
Dating Bulk Sediments from Limnic Deposits Using a Grain-Size Approach
Authors: Rothacker Leo , Dreves Alexander , Sirocko Frank , Grootes Pieter M , Nadeau Marie-Josée
Published: January 2017

Radiocarbon measurements on bulk subaqueous sediments typically provide ages significantly older than actual time of deposition. This is generally caused by the presence of reworked organic compounds, which are depleted in 14C. To explore this issue of age heterogeneity, we collected 4 organic-rich samples from varying depths in a lake sediment core at the Gemündener Maar (Eifel, Germany), a lake of volcanic origin. We divided each sample into 5 standard grain-size fractions: gravel, sand, silt, clay, and 1 fraction smaller than 0.45 μm. These were cleaned separately using a standard acid-alkali-acid treatment. The highly organic gravel-size fraction provided the youngest 14C ages of all grain-size fractions and seems to be associated most closely with the time of deposition. By contrast, the silt and clay fractions show significantly older ages. If the investigated limnic sediment layer does not contain any identifiable terrestrial macrofossils, extracting and measuring coarser grain-size fractions instead of measuring bulk sediment samples will provide a better approximation of the time of sedimentation.
Radiocarbon and Other Environmental Isotopes in the Groundwater of the Sites for a Planned New Nuclear Power Plant in Lithuania
Authors: Mažeika Jonas , Martma Tõnu , Petrošius Rimantas , Jakimavičiūtė-Maselienė Vaidotė , Skuratovič Žana
Published: January 2017

The assessment of construction sites for the new Visaginas Nuclear Power Plant (Visaginas NPP), including groundwater characterization, took place over the last few years. For a better understanding of the groundwater system, studies on radiocarbon; tritium; stable isotopes of hydrogen, oxygen, and carbon; and helium content were carried out at the location of the new NPP, at the Western and Eastern sites, as well as in the near-surface repository (NSR) site. Two critical depth zones in the Quaternary aquifer system were characterized by different groundwater residence times and having slightly different stable isotope features and helium content. The first shallow interval of the Quaternary multi-aquifer system consists of an unconfined aquifer and semiconfined aquifer. The second depth interval of the system is related to the lower Quaternary confined aquifer. Groundwater residence time in the first flow system was mainly based on tritium data and ranges from 6 to 60 yr. These aquifers are the most important in terms of safety assessment and are considered as a potential radionuclide transfer pathway in safety assessment. Groundwater residence time in the lower Quaternary aquifers based on 14C data varies from modern to several thousand years and in some intervals up to 10,500 yr.
Radiocarbon Reservoir Ages as Freshwater-Brine Monitors in Lake Lisan, Dead Sea System
Authors: Stein Mordechai , Lazar Boaz , Goldstein Steven L
Published: January 2017

A continuous and high-resolution record of the radiocarbon reservoir age (RA) has been recovered from the primary aragonites that were deposited from the last glacial Lake Lisan. The RA is calculated as the difference between the measured 14C “apparent” age in the aragonite and the atmospheric age at any particular time. The RA shows temporal decreases during the time interval of ≃28 to ≃18 ka cal BP. This behavior is attributed to a continuous addition of low RA-high bicarbonate freshwater into the high RA-Ca-chloride (low bicarbonate) brine solution filling the lake. The mixing of the brine with freshwater drives the precipitation of CaCO3 in the form of aragonite from the lake epilimnion (surface layer). The runoff-brine mixture in Lake Lisan is also reflected by the Sr/Ca ratios that are positively correlated with the RA. Nevertheless, the 14C content in the epilimnion did not drop at the same rate as the atmospheric value but rather remained nearly constant. We suggest that turbulent mixing with the much saltier hypolimnion (lower layer) across the hypolimnion/epilimnion interface at a depth of about 390 m below sea level, buffered the 14C content as well as the Sr and Ca concentrations in the aragonite precipitating solution. The RA-Sr/Ca related limnological model developed here opens the way to determine the reservoir-age-corrected atmospheric ages of Lisan Formation aragonites beyond 28 ka cal BP.
Distribution of Radiocarbon Ages in Soil Organic Matter by Thermal Fractionation
Authors: Plante Alain F , Beaupré Steven R , Roberts Mark L , Baisden Troy
Published: January 2017

Radiocarbon analysis is an important tool in quantifying soil organic matter (SOM) dynamics within the terrestrial carbon cycle. However, there is increasing appreciation that representing SOM as a single, homogeneous pool with a single, mean 14C concentration is inadequate. We investigate whether the differing patterns in CO2 release during ramped-temperature oxidation reflect organic matter of different ages, and hypothesize that thermally labile SOM (combusting at low temperatures) consists of younger carbon than thermally resistant organic matter. Topsoil samples under contrasting land uses (native vegetation and long-term cultivation) were selected for 14C analysis before and after acid fumigation for the removal of carbonates. Results of bulk 14C analyses showed a significant shift in 14C age from 0.944 Fm under native vegetation to 0.790 Fm under cultivation. Four to 5 “fractions” associated with different CO2-evolution regions were identified by thermal analysis and analyzed for 14C via modifications to NOSAMS' established “programmed temperature pyrolysis system,” in which discrete CO2 fractions evolved during ramped-temperature oxidation were isotopically characterized by a microwave gas ion source (GIS) continuous-flow AMS (CFAMS) system. Results showed that while acid fumigation removed soil carbonates, the treatment also significantly altered the thermograms and inferred SOM composition. While direct attribution of 14C values to individual peaks is somewhat confounded by overlapping temperature ranges for oxidation of unique populations of carbon, in general, thermally stable fractions of SOM appear to be 14C-depleted compared to thermally reactive (low temperature) fractions regardless of pretreatment.
Retinal A2A and A3 adenosine receptors modulate the components of the rat electroretinogram
Authors: JONSSON GUDMUNDUR , EYSTEINSSON THOR
Published: January 2017

Adenosine is a neuromodulator present in various areas of the central nervous system, including the retina. Adenosine may serve a neuroprotective role in the retina, based on electroretinogram (ERG) recordings from the rat retina. Our purpose was to assess the role of A2A and A3 adenosine receptors in the generation and modulation of the rat ERG. The flash ERG was recorded with corneal electrodes from Sprague Dawley rats. Agonists and antagonists for A2A and A3 receptors, and adenosine were injected (5 µl) into the vitreous. The effects on the components of the single flash scotopic and photopic ERGs were examined, and ERG flicker. Adenosine (0.5 mM) increased the mean amplitudes of the scotopic ERG a-waves (68 ± 8 to 97 ± 14 µV, P = 0.042), and b-waves (236 ± 38 µV to 305 ± 42 µV). A2A agonist CGS21680 (2 mM) reduced the mean amplitude of the ERG b-wave, from 298 ± 21 µV in response to the brightest stimulus to 212 ± 19 µV (P = 0.005), and mean scotopic oscillatory potentials (OPs) from 100 ± 9 µV to 47 ± 11 µV (P = 0.023). ZM241385 [4 mM], an A2A antagonist, decreased the scotopic b-wave of the ERG. A3 agonist 2-CI-IB-MECA (0.5 mM) increased the a-wave, while decreasing the scotopic and photopic ERG b-waves, and the scotopic OPs. A3 antagonist VUF5574 (1 mM) increased the mean amplitude of the scotopic a-wave (66 ± 8 to 140 ± 29 µV, P = 0.046) and b-wave (224 ± 20 to 312 ± 39 µV, P = 0.0037). No significant effects on ERG flicker were found. We conclude that retinal neurons containing A2A and/or A3 adenosine receptors contribute to the generation of the ERG a- and b-waves and OPs.
Accuracy of Prenatal Ultrasound in Detecting Growth Abnormalities in Triplets: A Retrospective Cohort Study
Authors: Sklar Cameron , Yaskina Maryna , Ross Sue , Naud Kentia
Published: January 2017

Significant management decisions in triplet pregnancies are made based mainly on ultrasound measurements of fetal growth, although there is a paucity of data examining the accuracy of fetal weight measurements in these gestations. To evaluate accuracy of prenatal ultrasound to diagnose growth abnormalities (intrauterine growth restriction, severe growth discordance) in triplet pregnancies, a retrospective cohort study of 78 triplet pregnancies (234 fetuses) delivered at a single tertiary hospital from January 2004 to May 2015 was performed. Growth percentiles from the last ultrasound were derived from estimated fetal weight using Hadlock's formula for each triplet. Growth discordance was calculated for each triplet set using the formula {(estimated fetal weight largest triplet - estimated fetal weight smallest)/estimated fetal weight largest}. These estimations were compared to birth weights. Sensitivity of ultrasound to predict ≥1 growth restricted fetus in a triplet set was 55.6% [95% CI 35.3, 74.5]; specificity was 100% [95% CI 93.0, 100]; positive predictive value (PPV) 100% [95% CI 74.7, 100]; negative predictive value (NPV) 81.0% [95% CI 73.2, 85.7%]. Sensitivity of ultrasound to detect fetal growth discordance >25% in a triplet set was 80.0% [95% CI 44.4, 97.5], specificity 94.1% [95% CI 85.6, 98.4]; PPV 66.7% [95% CI 42.4, 84.5]; NPV 97.0% [95% CI 90.2, 99.1]. Prenatal ultrasound currently remains the most reliable tool to screen for growth anomalies in triplet pregnancies; however, it appears to have less than ideal sensitivity, missing a number of cases of intra-uterine growth restriction and significant growth discordance.
Alexandra Witze Receives 2016 David Perlman Award for Excellence in Science Journalism–News
Authors:   AGU
Published: January 2017

Alexandra Witze received the 2016 David Perlman Award for Excellence in Science Journalism–News at the American Geophysical Union Fall Meeting Honors Ceremony, held on 14 December 2016 in San Francisco, Calif. The award recognizes "a journalist for a news story or series in any medium except books that makes information about the Earth and space sciences accessible and interesting to the general public."
An oxygen sensitive self-decision making engineered CAR T-cell
Authors: Juillerat Alexandre , Marechal Alan , Filhol Jean Marie , Valogne Yannick , Valton Julien , Duclert Aymeric , Duchateau Philippe , Poirot Laurent
Published: January 2017

A key to the success of chimeric antigen receptor (CAR) T-cell based therapies greatly rely on the capacity to identify and target antigens with expression restrained to tumor cells. Here we present a strategy to generate CAR T-cells that are only effective locally (tumor tissue), potentially also increasing the choice of targetable antigens. By fusing an oxygen sensitive subdomain of HIF1α to a CAR scaffold, we generated CAR T-cells that are responsive to a hypoxic environment, a hallmark of certain tumors. Along with the development of oxygen-sensitive CAR T-cells, this work also provides a basic framework to use a multi-chain CAR as a platform to create the next generation of smarter self-decision making CAR T-cells.
Hepatic alterations are accompanied by changes to bile acid transporter-expressing neurons in the hypothalamus after traumatic brain injury
Authors: Nizamutdinov Damir , DeMorrow Sharon , McMillin Matthew , Kain Jessica , Mukherjee Sanjib , Zeitouni Suzanne , Frampton Gabriel , Bricker Paul Clint S. , Hurst Jacob , Shapiro Lee A.
Published: January 2017

Annually, there are over 2 million incidents of traumatic brain injury (TBI) and treatment options are non-existent. While many TBI studies have focused on the brain, peripheral contributions involving the digestive and immune systems are emerging as factors involved in the various symptomology associated with TBI. We hypothesized that TBI would alter hepatic function, including bile acid system machinery in the liver and brain. The results show activation of the hepatic acute phase response by 2 hours after TBI, hepatic inflammation by 6 hours after TBI and a decrease in hepatic transcription factors, Gli 1, Gli 2, Gli 3 at 2 and 24 hrs after TBI. Bile acid receptors and transporters were decreased as early as 2 hrs after TBI until at least 24 hrs after TBI. Quantification of bile acid transporter, ASBT-expressing neurons in the hypothalamus, revealed a significant decrease following TBI. These results are the first to show such changes following a TBI, and are compatible with previous studies of the bile acid system in stroke models. The data support the emerging idea of a systemic influence to neurological disorders and point to the need for future studies to better define specific mechanisms of action.
Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
Authors: Bettini Sarah , Boutet-Robinet Elisa , Cartier Christel , Coméra Christine , Gaultier Eric , Dupuy Jacques , Naud Nathalie , Taché Sylviane , Grysan Patrick , Reguer Solenn , Thieriet Nathalie , Réfrégiers Matthieu , Thiaudière Dominique , Cravedi Jean-Pierre , Carrière Marie , Audinot Jean-Nicolas , Pierre Fabrice H. , Guzylack-Piriou Laurence , Houdeau Eric
Published: January 2017

Food-grade titanium dioxide (TiO2) containing a nanoscale particle fraction (TiO2-NPs) is approved as a white pigment (E171 in Europe) in common foodstuffs, including confectionary. There are growing concerns that daily oral TiO2-NP intake is associated with an increased risk of chronic intestinal inflammation and carcinogenesis. In rats orally exposed for one week to E171 at human relevant levels, titanium was detected in the immune cells of Peyer’s patches (PP) as observed with the TiO2-NP model NM-105. Dendritic cell frequency increased in PP regardless of the TiO2treatment, while regulatory T cells involved in dampening inflammatory responses decreased with E171 only, an effect still observed after 100 days of treatment. In all TiO2-treated rats, stimulation of immune cells isolated from PP showed a decrease in Thelper (Th)-1 IFN-γ secretion, while splenic Th1/Th17 inflammatory responses sharply increased. E171 or NM-105 for one week did not initiate intestinal inflammation, while a 100-day E171 treatment promoted colon microinflammation and initiated preneoplastic lesions while also fostering the growth of aberrant crypt foci in a chemically induced carcinogenesis model. These data should be considered for risk assessments of the susceptibility to Th17-driven autoimmune diseases and to colorectal cancer in humans exposed to TiO2from dietary sources.
Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models
Authors: Kurogi Syuji , Sekimoto Tomohisa , Funamoto Taro , Ota Tomomi , Nakamura Shihoko , Nagai Takuya , Nakahara Mai , Yoshinobu Kumiko , Araki Kimi , Araki Masatake , Chosa Etsuo
Published: January 2017

Despite numerous genetic studies on bone metabolism, understanding of the specific mechanisms is lacking. We developed an efficient screening system to identify novel genes involved in bone metabolism using mutant mouse strains registered with the Exchangeable Gene Trap Clones (EGTC) database. From 1278 trap clones in the EGTC database, 52 candidate lines were selected in the first screening, determined based on “EST profile”, “X-gal”, “Related article”, and “Novel gene”. For the second screening, bone morphometric analysis, biomechanical strength analysis, bone X-gal staining, etc. were performed on candidate lines. Forty-two male trap lines (80.8%) showed abnormalities with either bone morphometric analysis or biomechanical strength analysis. In the screening process, X-gal staining was significantly efficient (P = 0.0057). As examples, Lbr and Nedd4 trap lines selected using the screening system showed significant bone decrease and fragility, suggesting a relationship with osteoblast differentiation. This screening system using EGTC mouse lines is extremely efficient for identifying novel genes involved in bone metabolism. The gene trap lines identified as abnormal using this screening approach are highly likely to trap important genes for bone metabolism. These selected trap mice will be valuable for use as novel bio-resources in bone research.
Mercury enrichment indicates volcanic triggering of Valanginian environmental change
Authors: Charbonnier Guillaume , Morales Chloé , Duchamp-Alphonse Stéphanie , Westermann Stéphane , Adatte Thierry , Föllmi Karl B.
Published: January 2017

The Valanginian stage (Early Cretaceous) includes an episode of significant environmental changes, which are well defined by a positive δ13C excursion. This globally recorded excursion indicates important perturbations in the carbon cycle, which has tentatively been associated with a pulse in volcanic activity and the formation of the Paraná-Etendeka large igneous province (LIP). Uncertainties in existing age models preclude, however, its positive identification as a trigger of Valanginian environmental changes. Here we report that in Valanginian sediments recovered from a drill core in Wąwał (Polish Basin, Poland), and from outcrops in the Breggia Gorge (Lombardian Basin, southern Switzerland), and Orpierre and Angles (Vocontian Basin, SE France), intervals at or near the onset of the positive δ13C excursion are significantly enriched in mercury (Hg). The persistence of the Hg anomaly in Hg/TOC, Hg/phyllosilicate, and Hg/Fe ratios shows that organic-matter scavenging and/or adsorbtion onto clay minerals or hydrous iron oxides only played a limited role. Volcanic outgassing was most probably the primary source of the Hg enrichments, which demonstrate that an important magmatic pulse triggered the Valanginian environmental perturbations.
Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau
Authors: Liu Yang , Yan Caiyu , Matthew Cory , Wood Brennon , Hou Fujiang
Published: January 2017

Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key seasons in 2012 and 2013 from a range of potential sources, including: alpine meadows, dung patches, manure heaps and yak night pens, on the Qinghai-Tibetan Plateau. We also estimated the total annual global warming potential (GWP, CO2-equivalents) from family farm grazing yaks using our measured results and other published data. In this study, GHG fluxes per unit area from night pens and composting manure heaps were higher than from dung patches and alpine meadows. Increased moisture content and surface temperature of soil and manure were major factors increasing CO2 and CH4 fluxes. High contributions of CH4 and N2O (21.1% and 44.8%, respectively) to the annual total GWP budget (334.2 tonnes) strongly suggest these GHG other than CO2 should not be ignored when estimating GWP from the family farm grazing yaks on the Qinghai-Tibetan Plateau for the purposes of determining national and regional land use policies or compiling global GHG inventories.
Temporal variations in presynaptic release probability in the lateral habenula
Authors: Park Hoyong , Cheon Myunghyun , Kim Sungmin , Chung ChiHye
Published: January 2017

Rhythmicity plays an important role in a number of biological systems. The habenular complex is reported to contain an intrinsic molecular clock and to show rhythmic expression of circadian clock genes and proteins including per2/PER2. In this study, we observed that there is a temporal rhythmicity in the presynaptic efficacy of the lateral habenula (LHb) neurons. We collected a substantial number of recordings at different time points of the day during the light phase. The frequency and amplitude of spontaneous excitatory transmission were increased in the afternoon compared to recordings performed in the morning. In addition, the paired-pulse ratio and the success rate of minimal stimulation were also significantly different depending on the time of the recording. We did not see any significant differences in recordings obtained from pyramidal neurons of the hippocampus in the same brain slices. Taken together, our data indicates that the LHb exhibits intrinsic temporal oscillation in basal neurotransmission and in presynaptic release probability. Given the rapidly growing interest on the function of the LHb, more careful examination of synaptic transmission in the LHb is thus required.