bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Treatment Modalities for Dry Eye in Primary Sjögren’s Syndrome
Authors: Ahn Hyunmin , Ji Yong Woo , Jun Ikhyun , Kim Tae-im , Lee Hyung Keun , Seo Kyoung Yul
Published: January 2022

Purpose: To evaluate the effectiveness of different treatment modalities for dry eye in primary Sjögren’s syndrome with their potential overlapping influences. Methods: This study included 199 patients with newly diagnosed primary Sjögren’s syndrome from 2005 to 2020. Various treatment modalities for primary Sjögren’s syndrome were compared. Improvement of corneal staining based on Sjögren’s International Collaborative Clinical Alliance (SICCA) scores was the primary outcome. Results: The average follow-up period was 5.4 ± 3.1 (range, 2.0–14.1) years. Analysis of the individual treatments showed that punctal plug insertions in the lower and upper eyelids were strongly associated with improvement of SICCA scores (β = 2.70 and 1.80, p < 0.001 and <0.001, respectively). With ocular surface inflammation, corneal staining scores improved significantly with steroid eye drops. Prednisolone (1%) had the strongest association with improvement of corneal staining scores (β = 1.48, p < 0.001); this was based on the frequency of administration. Without ocular surface inflammation, diquafosol (3%), carbomer gel, and lanolin ointment were effective (β = 1.37, 1.06, and 1.17; p = 0.003, 0.003, and <0.001, respectively). Conclusions: Punctal plug insertion, primarily targeting aqueous deficiency, is the mainstay of the treatment for dry eye in primary Sjögren’s syndrome even in the presence of ocular surface inflammation. Furthermore, the effectiveness of treatment modalities for dry eye in primary Sjögren’s syndrome was dependent on the presence of ocular surface inflammation.
Influence Mechanism of Geomorphological Evolution in a Tidal Lagoon with Rising Sea Level
Authors: Kuang Cuiping , Fan Jiadong , Dong Zhichao , Zou Qingping , Cong Xin , Han Xuejian
Published: January 2022

A tidal lagoon system has multiple environmental, societal, and economic implications. To investigate the mechanism of influence of the geomorphological evolution of a tidal lagoon, the effect of critical erosion shear stress, critical deposition shear stress, sediment settling velocity, and initial bed elevation were assessed by applying the MIKE hydro- and morpho-dynamic model to a typical tidal lagoon, Qilihai Lagoon. According to the simulation results, without sediment supply, an increase of critical erosion, deposition shear stress, or sediment settling velocity gives rise to tidal networks with a stable terrain. Such an equilibrium state can be defined as when the change of net erosion has little variation, which can be achieved due to counter actions between the erosion and deposition effect. Moreover, the influence of the initial bed elevation depends on the lowest tidal level. When the initial bed elevation is below the lowest tidal level, the tidal networks tend to be fully developed. A Spearman correlation analysis indicated that the geomorphological evolution is more sensitive to critical erosion or deposition shear stress than sediment settling velocity and initial bed elevation. Exponential sea level rise contributes to more intensive erosion than the linear or the parabolic sea level rise in the long-term evolution of a tidal lagoon.
Anomaly Detection in Maritime AIS Tracks: A Review of Recent Approaches
Authors: Wolsing Konrad , Roepert Linus , Bauer Jan , Wehrle Klaus
Published: January 2022

The automatic identification system (AIS) was introduced in the maritime domain to increase the safety of sea traffic. AIS messages are transmitted as broadcasts to nearby ships and contain, among others, information about the identification, position, speed, and course of the sending vessels. AIS can thus serve as a tool to avoid collisions and increase onboard situational awareness. In recent years, AIS has been utilized in more and more applications since it enables worldwide surveillance of virtually any larger vessel and has the potential to greatly support vessel traffic services and collision risk assessment. Anomalies in AIS tracks can indicate events that are relevant in terms of safety and also security. With a plethora of accessible AIS data nowadays, there is a growing need for the automatic detection of anomalous AIS data. In this paper, we survey 44 research articles on anomaly detection of maritime AIS tracks. We identify the tackled AIS anomaly types, assess their potential use cases, and closely examine the landscape of recent AIS anomaly research as well as their limitations.
Research on the Water Ridge and Slamming Characteristics of a Semisubmersible Platform under Towing Conditions
Authors: Huo Fali , Wei Changdong , Zhu Chenyang , Yuan Zhaojun , Xu Sheng
Published: January 2022

During the towing of semisubmersible platforms, waves impact and superpose in front of the platform to form a ridge shaped “water ridge”, which protrudes near the platform and produces a large slamming pressure. The water ridges occur frequently in the towing conditions of semisubmersible platforms. The wave–slamming on the braces and columns of platform is aggravated due to the water ridges, particularly in rough sea conditions. The effect of water ridges is usually ignored in slamming pressure analysis, which is used to check the structural strengths of the braces and columns. In this paper, the characteristics of the water ridge at the braces of a semisubmersible platform are studied by experimental tests and numerical simulations. In addition, the sensitivity of the water ridge to the wave height and period is studied. The numerical simulations are conducted by a Computational Fluid Dynamics (CFD) method, and their accuracy is validated based on experimental tests. The characteristics of the water ridge and slamming pressure on the braces and columns are studied in different wave conditions based on the validated numerical model. It is found that the wave extrusion is the main reason of water ridge. The wave–slamming pressure caused by the water ridge has an approximately linear increase with the wave height and is sensitive to the wave period. With the increase of the wave period, the wave–slamming pressure on the brace and column of the platform increases first and then decreases. The maximum wave–slamming pressure is found when the wave period is 10 s and the slamming pressure reduces rapidly with an increase of wave period.
Modular Approach in the Design of Small Passenger Vessels for Mediterranean
Authors: Vladimir Nikola , Bakica Andro , Perčić Maja , Jovanović Ivana
Published: January 2022

This paper deals with the modular concept in the design of small passenger vessels for the Mediterranean, where the ship is assembled from three virtual modules (hull, power system and superstructure), enabling different vessel characteristics (speed, capacity, environmental performance, habitability, etc.). A set of predefined modules is established based on the investigation of market needs, where the IHS Fairplay database is taken as a reference for ship particulars and power needs, while the set of environmental regulation scenarios and requirements on ship habitability are taken as relevant for the design of ship power systems and superstructure modules, respectively. For the selected hull, a series of computations have been conducted to obtain their resistance and power needs which are further satisfied in the above-described manner. Within the illustrative example, a small passenger vessel with a capacity of 250 passengers is considered, with a detailed description of relevant modules that fit future design requirement scenarios. This approach is aimed at small-scale shipyards with limited research capabilities, who can quickly obtain the preliminary design of the vessel which can be further optimized to the final solution.
Methods and Proposals for Solutions in the Applicability of a Software-Defined Radio in Maritime Communication
Authors: Bistrović Miroslav , Komorčec Domagoj
Published: January 2022

Ship communication systems are defined in one part as GMDSS, while the other part consists of systems with secondary importance in safety communications. Each of these devices and systems makes an independent or separate system that works in a specific frequency range and is, at some level, connected to other communication systems. A step forward regarding frequency range and level of networking can be achieved with the application of a software-defined radio. This paper examines a variety of GMDSS communication equipment in terms of technical discrepancy and frequency range. Furthermore, the software-defined radio and SDR configuration development are described according to their theoretical feasibility in the maritime domain. The paper proposes the concept of SDR-based communication systems quite different from conventional maritime communication systems. This approach, conducted in phases, would in turn ease the upgrading, enable flexibility and inter-operation, prolong system life cycle and integrate different maritime communication systems and devices. The proposed concept aims to develop a centralized communication system to incorporate the ship’s communication devices into one common ship communication system.
Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans
Authors: Gila Barnabás Cs. , Antal Károly , Birkó Zsuzsanna , Keserű Judit Sz. , Pócsi István , Emri Tamás
Published: January 2022

Understanding the coordinated regulation of the hundreds of carbohydrate-active enzyme (CAZyme) genes occurring in the genomes of fungi has great practical importance. We recorded genome-wide transcriptional changes of Aspergillus nidulans cultivated on glucose, lactose, or arabinogalactan, as well as under carbon-starved conditions. We determined both carbon-stress-specific changes (weak or no carbon source vs. glucose) and carbon-source-specific changes (one type of culture vs. all other cultures). Many CAZyme genes showed carbon-stress-specific and/or carbon-source-specific upregulation on arabinogalactan (138 and 62 genes, respectively). Besides galactosidase and arabinan-degrading enzyme genes, enrichment of cellulolytic, pectinolytic, mannan, and xylan-degrading enzyme genes was observed. Fewer upregulated genes, 81 and 107 carbon stress specific, and 6 and 16 carbon source specific, were found on lactose and in carbon-starved cultures, respectively. They were enriched only in galactosidase and xylosidase genes on lactose and rhamnogalacturonanase genes in both cultures. Some CAZyme genes (29 genes) showed carbon-source-specific upregulation on glucose, and they were enriched in β-1,4-glucanase genes. The behavioral ecological background of these characteristics was evaluated to comprehensively organize our knowledge on CAZyme production, which can lead to developing new strategies to produce enzymes for plant cell wall saccharification.
Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
Authors: Li Yongmei , Xia Mengyuan , He Pengbo , Yang Qiaoming , Wu Yixin , He Pengfei , Ahmed Ayesha , Li Xiangsong , Wang Yuehu , Munir Shahzad , He Yueqiu
Published: January 2022

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.
Strategies for Efficient Expression of Heterologous Monosaccharide Transporters in Saccharomyces cerevisiae
Authors: Knychala Marilia M. , dos Santos Angela A. , Kretzer Leonardo G. , Gelsleichter Fernanda , Leandro Maria José , Fonseca César , Stambuk Boris U.
Published: January 2022

In previous work, we developed a Saccharomyces cerevisiae strain (DLG-K1) lacking the main monosaccharide transporters (hxt-null) and displaying high xylose reductase, xylitol dehydrogenase and xylulokinase activities. This strain proved to be a useful chassis strain to study new glucose/xylose transporters, as SsXUT1 from Scheffersomyces stipitis. Proteins with high amino acid sequence similarity (78–80%) to SsXUT1 were identified from Spathaspora passalidarum and Spathaspora arborariae genomes. The characterization of these putative transporter genes (SpXUT1 and SaXUT1, respectively) was performed in the same chassis strain. Surprisingly, the cloned genes could not restore the ability to grow in several monosaccharides tested (including glucose and xylose), but after being grown in maltose, the uptake of 14C-glucose and 14C-xylose was detected. While SsXUT1 lacks lysine residues with high ubiquitinylation potential in its N-terminal domain and displays only one in its C-terminal domain, both SpXUT1 and SaXUT1 transporters have several such residues in their C-terminal domains. A truncated version of SpXUT1 gene, deprived of the respective 3′-end, was cloned in DLG-K1 and allowed growth and fermentation in glucose or xylose. In another approach, two arrestins known to be involved in the ubiquitinylation and endocytosis of sugar transporters (ROD1 and ROG3) were knocked out, but only the rog3 mutant allowed a significant improvement of growth and fermentation in glucose when either of the XUT permeases were expressed. Therefore, for the efficient heterologous expression of monosaccharide (e.g., glucose/xylose) transporters in S. cerevisiae, we propose either the removal of lysines involved in ubiquitinylation and endocytosis or the use of chassis strains hampered in the specific mechanism of membrane protein turnover.
Impact of Soil Chemical Properties on the Growth Promotion Ability of Trichoderma ghanense, T. tomentosum and Their Complex on Rye in Different Land-Use Systems
Authors: Bridžiuvienė Danguolė , Raudonienė Vita , Švedienė Jurgita , Paškevičius Algimantas , Baužienė Ieva , Vaitonis Gintautas , Šlepetienė Alvyra , Šlepetys Jonas , Kačergius Audrius
Published: January 2022

Microbial-based biostimulants that increase plant performance and ensure sustainable restoration of degraded soils are of great importance. The aim of the present study was to evaluate the growth promotion ability of indigenous Trichoderma ghanense, T. tomentosum and their complex on early rye seedlings in sustained grassland and arable soil. The impact of soil chemical properties on the ability of selected Trichoderma strains and their complex to promote plant growth was determined by the evaluation of the rye (Secale cereale L.) early seedling growth—measuring the length of shoots and roots as well as their dry weight. Trichoderma species were tested for their ability to produce extracellular degradative enzymes on solid media. Furthermore, the soil properties and CM-cellulase activity of soil were estimated. The indigenous Trichoderma strains possess the capacity to produce enzymes such as peroxidase, laccase, tyrosinase, and endoglucanase. The results indicated a significant (p < 0.05) increase in plant growth and the improvement of some soil chemical properties (total N, mobile humic and fulvic acids, exchangeable K2O, soil CM-cellulase activity) in inoculated soils when compared to the control. The growth of the roots of rye seedlings in sustained grassland was enhanced when T. tomentosum was applied (p = 0.005). There was an increase in total weight and shoot weight of rye seedlings when T. ghanense was used in the arable soil (p = 0.014 and p = 0.024). The expected beneficial effect of Trichoderma spp. complex on rye growth promotion was not observed in any tested soil. The results could find application in the development of new and efficient biostimulants, since not only do physiological characteristics of fungi play an important role but also the quality of the soil has an impact.
T2Candida Assay in the Diagnosis of Intraabdominal Candidiasis: A Prospective Multicenter Study
Authors: Krifors Anders , Ullberg Måns , Castegren Markus , Petersson Johan , Sparrelid Ernesto , Hammarström Helena , Sjölin Jan , Özenci Volkan , Blennow Ola
Published: January 2022

The T2Candida magnetic resonance assay is a direct-from-blood pathogen detection assay that delivers a result within 3–5 h, targeting the most clinically relevant Candida species. Between February 2019 and March 2021, the study included consecutive patients aged >18 years admitted to an intensive care unit or surgical high-dependency unit due to gastrointestinal surgery or necrotizing pancreatitis and from whom diagnostic blood cultures were obtained. Blood samples were tested in parallel with T2Candida and 1,3-β-D-glucan. Of 134 evaluable patients, 13 (10%) were classified as having proven intraabdominal candidiasis (IAC) according to the EORTC/MSG criteria. Two of the thirteen patients (15%) had concurrent candidemia. The sensitivity, specificity, positive predictive value, and negative predictive value, respectively, were 46%, 97%, 61%, and 94% for T2Candida and 85%, 83%, 36%, and 98% for 1,3-β-D-glucan. All positive T2Candida results were consistent with the culture results at the species level, except for one case of dual infection. The performance of T2Candida was comparable with that of 1,3-β-D-glucan for candidemic IAC but had a lower sensitivity for non-candidemic IAC (36% vs. 82%). In conclusion, T2Candida may be a valuable complement to 1,3-β-D-glucan in the clinical management of high-risk surgical patients because of its rapid results and ease of use.
A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches
Authors: Kiselev Andrei , San Clemente Hélène , Camborde Laurent , Dumas Bernard , Gaulin Elodie
Published: January 2022

The soil-borne oomycete pathogen Aphanomyces euteiches causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of A. euteiches have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of A. euteiches ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of A. euteiches is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of A. euteiches with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of A. euteiches with legumes.
In Service of News Subscribers: Exploring Belgian Journalists’ Perceptions of Stakeholder Relations in the Digital News Ecosystem
Authors: Luyckx Dorien , Paulussen Steve
Published: January 2022

This qualitative paper contends that as news media are faced with growing commercial pressures and changing news consumption habits, they need to rethink their relationship with two of their main stakeholders: readers and advertisers. Multi-stakeholder marketing provides a useful conceptual framework for such an exercise, since it invites media practitioners to reconcile the conflicting interests of different stakeholders. This study aims to understand journalists’ levels of multi-stakeholder thinking regarding advertisers and readers. To explore how contemporary journalists see their role with regard to distinct stakeholders in the news ecosystem, we interviewed 14 Belgian journalists working for legacy and digital native news media. The goal of this exploratory study is to examine (1) how journalists perceive and rethink their dependence on readers and advertisers in the digital news ecosystem and (2) how their perception of the digital news ecosystem influences their attitudes towards these stakeholders. Findings indicate that journalists tend to see the value of readers in monetary terms and believe increasing reader revenue will help journalism survive. Other types of reader value (feedback, expertise, and content) are peripheral. This focus on subscribers also seems to coincide with a devaluation of other stakeholders like advertisers and non-paying readers.
The Relationship between Epigenetic Age and Myocardial Infarction/Acute Coronary Syndrome in a Population-Based Nested Case-Control Study
Authors: Malyutina Sofia , Chervova Olga , Tillmann Taavi , Maximov Vladimir , Ryabikov Andrew , Gafarov Valery , Hubacek Jaroslav A. , Pikhart Hynek , Beck Stephan , Bobak Martin
Published: January 2022

We investigated the relationship between ‘epigenetic age’ (EA) derived from DNA methylation (DNAm) and myocardial infarction (MI)/acute coronary syndrome (ACS). A random population sample was examined in 2003/2005 (n = 9360, 45–69, the HAPIEE project) and followed up for 15 years. From this cohort, incident MI/ACS (cases, n = 129) and age- and sex-stratified controls (n = 177) were selected for a nested case-control study. Baseline EA (Horvath’s, Hannum’s, PhenoAge, Skin and Blood) and the differences between EA and chronological age (CA) were calculated (ΔAHr, ΔAHn, ΔAPh, ΔASB). EAs by Horvath’s, Hannum’s and Skin and Blood were close to CA (median absolute difference, MAD, of 1.08, –1.91 and –2.03 years); PhenoAge had MAD of −9.29 years vs. CA. The adjusted odds ratios (ORs) of MI/ACS per 1–year increments of ΔAHr, ΔAHn, ΔASB and ΔAPh were 1.01 (95% CI 0.95–1.07), 1.01 (95% CI 0.95–1.08), 1.02 (95% CI 0.97–1.06) and 1.01 (0.93–1.09), respectively. When classified into tertiles, only the highest tertile of ΔAPh showed a suggestion of increased risk of MI/ACS with OR 2.09 (1.11–3.94) independent of age and 1.84 (0.99–3.52) in the age- and sex-adjusted model. Metabolic modulation may be the likely mechanism of this association. In conclusion, this case-control study nested in a prospective population-based cohort did not find strong associations between accelerated epigenetic age markers and risk of MI/ACS. Larger cohort studies are needed to re-examine this important research question.
Predictors of the Intention to Be Vaccinated against COVID-19 in a Sample of Italian Respondents at the Start of the Immunization Campaign
Authors: Santirocchi Alessandro , Spataro Pietro , Costanzi Marco , Doricchi Fabrizio , Rossi-Arnaud Clelia , Cestari Vincenzo
Published: January 2022

COVID-19 vaccines are the most promising means of limiting the pandemic. The present study aims at determining the roles of several psychological variables in predicting vaccination intention in Italy. An online questionnaire was disseminated between 9 March and 9 May 2021. The sample included 971 participants. Results showed that most of the participants were willing to vaccinate. Acceptance rates were correlated with age, marital status, and area of residence. Intention to be vaccinated was positively correlated with perceived risk, pro-sociality, fear of COVID-19, use of preventive behaviors, and trust in government, in science, and in medical professionals. Intention to be vaccinated was negatively associated with belief in misinformation. The degree of acceptance is likely to be a result of the campaign tailored to address people’s negative attitudes towards vaccines. Trust in government and trust in science were among the strongest psychological predictors of vaccination intention. Fear of COVID-19, but not perceived risk, was associated with increased vaccine uptake, suggesting that the affective component of risk perception was more important than the cognitive component in predicting participants’ behaviors. Belief in misinformation was associated with reduced vaccination intention. Future studies will take into consideration these variables, to better understand the multifaceted process underlying vaccination intention.
“Spazio Huntington”: Tracing the Early Motor, Cognitive and Behavioral Profiles of Kids with Proven Pediatric Huntington Disease and Expanded Mutations > 80 CAG Repeats
Authors: Graziola Federica , Maffi Sabrina , Grasso Melissa , Garone Giacomo , Migliore Simone , Scaricamazza Eugenia , Ceccarelli Consuelo , Casella Melissa , Busi Ludovica , D’Alessio Barbara , De Luca Alessandro , Colafati Giovanna Stefania , Sabatini Umberto , Capuano Alessandro , Squitieri Ferdinando
Published: January 2022

The “Spazio Huntington—A Place for Children” program was launched in 2019. The aim was to contact at risk kids within Huntington disease (HD) families, to provide counseling to their parents and to start a prospective follow-up of kids suspicious to manifest pediatric HD (PHD). We met 25 at risk kids in two years, four of whom with PHD and highly expanded (HE) mutations beyond 80 CAG repeats. We rated motor, neuropsychological and behavioral changes in all PHD kids by the Unified HD Rating Scale (UHDRS)-total motor score (TMS) and additional measures of (1) cognitive level (Leiter International Performance Scale), (2) adaptive functioning (Adaptive Behavior Assessment Systems), (3) receptive language (Peabody Picture Vocabulary Test) and (4) behavioral abnormalities (Child Behavior Check List and Children’s Yale–Brown Obsessive Compulsive Scale). All PHD kids showed a severe progression of neurological and psychiatric manifestations including motor, cognitive and behavioral changes. The magnetic resonance imaging contributed to confirm the suspicious clinical observation by highlighting very initial striatum abnormalities in PHD. Spazio Huntington is a program to prospectively study PHD, the most atypical face of HD, and may represent the basis to recruit PHD patients in future clinical trials.
Ethnical Differences in Knee Phenotypes Indicate the Need for a More Individualized Approach in Knee Arthroplasty: A Comparison of 80 Asian Knees with 308 Caucasian Knees
Authors: Moser Lukas B. , Hess Silvan , de Villeneuve Bargemon Jean-Baptiste , Faizan Ahmad , LiArno Sally , Amsler Felix , Hirschmann Michael T. , Ollivier Matthieu
Published: January 2022

The purpose of this study was to determine the distribution of functional knee phenotypes in a non-osteoarthritic Asian population. The Stryker Orthopaedic Modeling and Analytics (SOMA) database was searched for CT scans of the lower limb meeting the following inclusion criteria: patient age at time of image >18 and <46 years, no signs of fractures and/or previous surgery and/or degenerative changes, Asian ethnicity. A total of 80 CT scans of 40 patients were included (24 males, 16 females). The hip-knee-ankle angle (HKA), femoral mechanical angle (FMA) and tibial mechanical angle (TMA) were measured. Based on these measurements, each limb was phenotyped according to the previously introduced functional knee phenotype concept. All angles and phenotypes of the present study were compared with previously published data of a non-osteoarthritic Caucasian population (308 legs of 160 patients, 102 males, 58 females). Asian knees had a significantly lower TMA (both genders p < 0.001) but a higher FMA (males p < 0.05, females p < 0.001) than Caucasian knees but showed no difference in the HKA. Asian knees differed significantly with regard to femoral and tibial phenotypes (p < 0.01), but not with regard to limb phenotypes. The high variability of all coronal alignment parameters highlights the importance of a detailed analysis prior to TKA. Ethnical differences underline the need for a more individualized approach in TKA.
The Central Arizona Conservation Alliance Programs: Use of Social Media and App-Supported Community Science for Landscape-Scale Habitat Restoration, Governance Support, and Community Resilience-Building
Authors: Raschke Aireona B. , Davis Jeny , Quiroz Annia
Published: January 2022

Land managers are currently faced with a nexus of challenges, both ecological and social, when trying to govern natural open spaces. While social media has led to many challenges for effective land management and governance, the technology has the potential to support key activities related to habitat restoration, awareness-raising for policy changes, and increased community resilience as the impacts of increased use and climate change become more apparent. Through the use of a case study examining the work of the Central Arizona Conservation Alliance’s social media ambassadorship and its app-supported community science projects, we examine the potential and realized positive impact that technology such as social media and smartphone apps can create for land managers and surrounding communities.
Intangible Cultural Heritage in Tourism: Research Review and Investigation of Future Agenda
Authors: Qiu Qihang , Zuo Yifan , Zhang Mu
Published: January 2022

Intangible cultural heritage (ICH) can be a valuable tourism resource for both government and local communities. However, the complex definition and the massive and fragmented nature of ICH data make it hard to review and conclude research trends and future directions of ICH tourism. In this study, 85 keywords extracted from ICH definitions are input in the Web of Science database before collecting papers indexed in the Social Sciences Citation Index, the Arts and Humanities Citation Index, and the Conference Proceedings Citation Index-Social Science and Humanities. Later, a systematic literature review of 418 ICH tourism studies from 76 countries published between 2000 and 2021 were conducted based on three groups of questions. The findings mainly illustrated that: (1) Currently research in ICH tourism is mainly composed of three themes: resource planning and sustainability, the impact of tourism development, and tourist behavior and destination marketing; (2) topics related to food tourism, sacred knowledge, traditional management systems, traditional management systems, legends, and myths can achieve high impact; (3) in the last five years, scholars have reduced using the official full name of ICH in tourism studies, while the category of “social practices, rituals and festive events” has become a hot topic since 2010; (4) ecotourism, culinary tourism, festival tourism, and religious tourism are the most discussed in ICH tourism research, and they will still be intensive topics in near future; (5) future directions in ICH tourism research are resultant of three vectors: place making, technology, and environment. The results present a comprehensive picture of current popular ICH topics and predict future directions in the field of ICH tourism. The systematic review of literature can help contribute to both theoretical construction, heritage preservation, and tourism practices.
Intestinal Disorder in Zebrafish Larvae (Danio rerio): The Protective Action of N-Palmitoylethanolamide-oxazoline
Authors: Di Paola Davide , Natale Sabrina , Iaria Carmelo , Cordaro Marika , Crupi Rosalia , Siracusa Rosalba , D’Amico Ramona , Fusco Roberta , Impellizzeri Daniela , Cuzzocrea Salvatore , Spanò Nunziacarla , Gugliandolo Enrico , Peritore Alessio Filippo
Published: January 2022

IBD (Inflammatory Bowel Disease) is an inflammatory disease affecting the gastrointestinal tract that is common in both humans and veterinarians. Several studies have revealed the pharmacological properties of the oxazoline of palmitoylethanolamide (PEAOXA). Zebrafish larvae were exposed to sodium dextran sulphate (DSS) to induce enterocolitis and study the protective action of PEAOXA. After repetitive exposure with 0.25% DSS, larvae presented gut alteration with an increase in mucus production. Furthermore, DSS exposure induced an increase in the inflammatory pathway in the intestine, related to an increase in the Endoplasmic-reticulum (ER) stress genes. PEAOXA exposure at a concentration of 10 mg/L decreased the DSS-induced gut damage and mucus production, as well as being able to reduce the inflammatory and ER stress-related genes expression. In conclusion, our results demonstrate that the alterations induced by repeated exposure to DSS were counteracted by PEAOXA action that was able to inhibit the increase in inflammation and ER stress involved in the progression of enterocolitis.
A Severe Acute Pancreatitis Mouse Model Transited from Mild Symptoms Induced by a “Two-Hit” Strategy with L-Arginine
Authors: Yang Jing , Tang Xujiao , Wu Qingqing , Ren Panpan , Yan Yishu
Published: January 2022

To develop a severe acute pancreatitis (SAP) model transited from mild symptoms, we investigated a “two-hit” strategy with L-arginine in mice. The mice were intraperitoneally injected with ice-cold L-arginine (4 g/kg) twice at an interval of 1 h on the first day and subjected to the repeated operation 72 h afterwards. The results showed the “two-hit” strategy resulted in the destructive damage and extensive necrosis of acinar cells in the pancreas compared with the “one-hit” model. Meanwhile, excessive levels of pro-inflammatory mediators, namely IL-6 and TNF-α, were released in the serum. Remarkably, additional deleterious effects on multiple organs were observed, including high intestinal permeability, kidney injury, and severe acute lung injury. Therefore, we confirmed that the SAP animal model triggered by a “two-hit” strategy with L-arginine was successfully established, providing a solid foundation for a deeper understanding of SAP initiation and therapy research to prevent worsening of the disease.
TAM Receptors in the Pathophysiology of Liver Disease
Authors: Flint Emilio , Triantafyllou Evangelos , Bernsmeier Christine
Published: January 2022

TAM receptors (Tyro3, Axl and MerTK) are a family of tyrosine kinase receptors that are expressed in a variety of cell populations, including liver parenchymal and non-parenchymal cells. These receptors are vital for immune homeostasis, as they regulate the innate immune response by suppressing inflammation via toll-like receptor inhibition and by promoting tissue resolution through efferocytosis. However, there is increasing evidence indicating that aberrant TAM receptor signaling may play a role in pathophysiological processes in the context of liver disease. This review will explore the roles of TAM receptors and their ligands in liver homeostasis as well as a variety of disease settings, including acute liver injury, steatosis, fibrosis, cirrhosis-associated immune dysfunction and hepatocellular carcinoma. A better understanding of our current knowledge of TAM receptors in liver disease may identify new opportunities for disease monitoring as well as novel therapeutic targets. Nonetheless, this review also aims to highlight areas where further research on TAM receptor biology in liver disease is required.
Co-Doped Magnesium Oxychloride Composites with Unique Flexural Strength for Construction Use
Authors: Lauermannová Anna-Marie , Jankovský Ondřej , Lojka Michal , Faltysová Ivana , Slámová Julie , Pavlíková Milena , Pivák Adam , Marušiak Šimon , Pavlík Zbyšek , Záleská Martina
Published: January 2022

In this study, the combined effect of graphene oxide (GO) and oxidized multi-walled carbon nanotubes (OMWCNTs) on material properties of the magnesium oxychloride (MOC) phase 5 was analyzed. The selected carbon-based nanoadditives were used in small content in order to obtain higher values of mechanical parameters and higher water resistance while maintaining acceptable price of the final composites. Two sets of samples containing either 0.1 wt. % or 0.2 wt. % of both nanoadditives were prepared, in addition to a set of reference samples without additives. Samples were characterized by X-ray diffraction, scanning electron microscopy, Fourier-transform infrared spectroscopy, and energy dispersive spectroscopy, which were used to obtain the basic information on the phase and chemical composition, as well as the microstructure and morphology. Basic macro- and micro-structural parameters were studied in order to determine the effect of the nanoadditives on the open porosity, bulk and specific density. In addition, the mechanical, hygric and thermal parameters of the prepared nano-doped composites were acquired and compared to the reference sample. An enhancement of all the mentioned types of parameters was observed. This can be assigned to the drop in porosity when GO and OMWCNTs were used. This research shows a pathway of increasing the water resistance of MOC-based composites, which is an important step in the development of the new generation of construction materials.
Status and Challenges in Homogenization Methods for Lattice Materials
Authors: Somnic Jacobs , Jo Bruce W.
Published: January 2022

Lattice structures have shown great potential in that mechanical properties are customizable without changing the material itself. Lattice materials could be light and highly stiff as well. With this flexibility of designing structures without raw material processing, lattice structures have been widely used in various applications such as smart and functional structures in aerospace and computational mechanics. Conventional methodologies for understanding behaviors of lattice materials take numerical approaches such as FEA (finite element analysis) and high-fidelity computational tools including ANSYS and ABAQUS. However, they demand a high computational load in each geometry run. Among many other methodologies, homogenization is another numerical approach but that enables to model behaviors of bulk lattice materials by analyzing either a small portion of them using numerical regression for rapid processing. In this paper, we provide a comprehensive survey of representative homogenization methodologies and their status and challenges in lattice materials with their fundamentals.
Investigation of the Mechanical and Liquid Absorption Properties of a Rice Straw-Based Composite for Ayurvedic Treatment Tables
Authors: Madival Abhishek Sadananda , Doreswamy Deepak , Handady Shripathi Adiga , Hebbar Krishna Raghava , Lakshminarayana Shobha Karabylu
Published: January 2022

Managing rice crop stubble is one of the major challenges witnessed in the agricultural sector. This work attempts to investigate the physical, mechanical, and liquid absorption properties of rice straw (RS)-reinforced polymer composite for assessing its suitability to use as an ayurvedic treatment table. This material is expected to be an alternative for wooden-based ayurvedic treatment tables. The results showed that the addition of rice straw particles (RSp) up to 60% volume in epoxy reduced the density of the composite material by 46.20% and the hardness by 15.69%. The maximum tensile and flexural strength of the RSp composite was 17.53 MPa and 43.23 MPa, respectively. The scanning electron microscopy (SEM) analysis showed deposits of silica in the form of phytoliths in various size and shapes on the outer surface of RS. The study also revealed that the water absorption rate (WA) was less than 7.8% for the test samples with 45% volume of RSp. Interestingly the test samples showed greater resistance to the absorption of Kottakal Dhanvantaram Thailam (<2%). In addition, the developed samples showed resistance towards bacterial and fungal growth under the exposure of treatment oils and water.
Flexible Thermoelectric Generator Based on Polycrystalline SiGe Thin Films
Authors: Ozawa Tomoki , Murata Masayuki , Suemasu Takashi , Toko Kaoru
Published: January 2022

Flexible and reliable thermoelectric generators (TEGs) will be essential for future energy harvesting sensors. In this study, we synthesized p- and n-type SiGe layers on a high heat-resistant polyimide film using metal-induced layer exchange (LE) and demonstrated TEG operation. Despite the low process temperature (<500 °C), the polycrystalline SiGe layers showed high power factors of 560 µW m−1 K−2 for p-type Si0.4Ge0.6 and 390 µW m−1 K−2 for n-type Si0.85Ge0.15, owing to self-organized doping in LE. Furthermore, the power factors indicated stable behavior with changing measurement temperature, an advantage of SiGe as an inorganic material. An in-plane π-type TEG based on these SiGe layers showed an output power of 0.45 µW cm−2 at near room temperature for a 30 K temperature gradient. This achievement will enable the development of environmentally friendly and highly reliable flexible TEGs for operating micro-energy devices in the future Internet of Things.
Multi-Dimensional Revealing the Influence Mechanism of the δ Phase on the Tensile Fracture Behavior of a Nickel-Based Superalloy on the Mesoscopic Scale
Authors: Zhu Qiang , Zhang Linfu , Wang Chuanjie , Chen Gang , Qin Heyong , Zhang Peng
Published: January 2022

As the key materials of aircraft engines, nickel-based superalloys have excellent comprehensive properties. Mircotensile experiments were carried out based on in situ digital image correlation (DIC) and in situ synchrotron radiation (SR) technique. The effects of the δ phase on the grain orientation, surface roughening, and strain localization were investigated. The results showed that the average kernel average misorientation (KAM) value of the fractured specimens increased significantly compared with that of the heat-treated specimens. The surface roughness decreased with an increasing volume fraction of the δ phase. The strain localization of specimens increased with the increasing ageing time. The size and volume fraction of voids gradually increased with the increase in plastic strain. Some small voids expanded into large voids with a complex morphology during micro-tensile deformation. The needle-like δ phase near the fracture broke into short rods, while the minor spherical δ phase did not break. The rod-like and needle-like δ phases provided channels for the propagation of the microcrack, and the accumulation of the microcrack eventually led to the fracture of specimens.
The Effect of Various Grinding Aids on the Properties of Cement and Its Compatibility with Acrylate-Based Superplasticizer
Authors: Kapeluszna Ewa , Kotwica Łukasz
Published: January 2022

The influence of grinding aids (pure triethanolamine and ethylene glycol) on the properties of cements, their compatibility with an acrylate-based superplasticizer and the rheological parameters of mortars were investigated. The presence of surfactants influences the standard properties of cements and the effectiveness of the superplasticizer. The results of the heat of hydration and setting time measurements indicate a delay in the hydration process and an increase in the induction period duration of the surfactant-doped pastes, in relation to the reference sample without grinding aids. Triethanolamine increases early-age compressive strength; the effect was observed for both standard and superplasticizer-containing mortars. The presence of grinding aids decreases the slump flow of mortars and increases rheological parameters such as yield stress (τ0) and viscosity (η).
High Temperature Oxidation Behavior of Creep Resistant Steels in Water Vapour Containing Environments
Authors: Hagarová Mária , Baranová Gabriela , Fujda Martin , Matvija Miloš , Horňak Peter , Bednarčík Jozef , Yudina Daria
Published: January 2022

This study describes the water vapour effect on the oxidation resistance of 9Cr creep resistant steels. Boiler P91 and MarBN steels were oxidized for 3000 h in a simulated humid atmosphere with ~10% water vapour. The oxidation kinetics had a stable course for 1000 h and was evaluated by the weight gain curves for both experimental steels and both oxidation temperatures. The oxidation rate was higher at 650 °C versus 600 °C, as reflected by the oxidation rate coefficient. A significant increase occurred after 1000 h of oxidation, which was related to the local breakdown oxide scale and oxide nodules were formed on steel. This oxidation behavior was influenced by the fact that a compact spinel structure of iron oxides and alloying elements were not formed on the steel. Analysis after 3000 h of exposure showed hematite Fe2O3 formed on the outer layer, magnetite Fe3O4 on the middle layer, and the bottom layer consisted of iron-chromium-spinel (Fe,Cr)2O3.
Study on Generating Machining Performance of Two-Dimensional Ultrasonic Vibration-Composited Electrolysis/Electro-Discharge Technology for MMCs
Authors: Li Jing , Chen Wanwan , Zhu Yongwei
Published: January 2022

Ultrasonic vibration-composited electrolysis/electro-discharge machining technology (UE/DM) is effective for machining particulate-reinforced metal matrix composites (MMCs). However, the vibration of the tool or workpiece suitable for holes limits the application of UE/DM. To improve the generating machining efficiency and quality of flat and curved surfaces, in this study, we implemented two-dimensional ultrasonic vibration into UE/DM and constructed a novel method named two-dimensional ultrasonic vibration-composited electrolysis/electro-discharge machining (2UE/DM). The influence of vibration on the performance of 2UE/DM compared to other process technologies was studied, and an orthogonal experiment was designed to optimize the parameters. The results indicated that the materiel remove rate (MRR) mainly increased via voltage and tool vibration. The change current was responsible for the MRR in the process. Spindle speed and workpiece vibration were not dominant factors affecting the MRR; the spindle speed and tool and workpiece vibration, which reduced the height difference between a ridge and crater caused by abrasive grinding, were responsible for surface roughness (Ra) and form precision (δ). Additionally, the optimized parameters of 1000 rpm, 3 V, and 5 um were conducted on MMCs of 40 SiCp/Al and achieved the maximum MRR and minimum Ra and δ of 0.76 mm3/min, 3.35 um, and 5.84%, respectively. This study’s findings provide valuable process parameters for improving machining efficiency and quality for MMCs of 2UE/DM.
Real-Time Sensing of Output Polymer Flow Temperature and Volumetric Flowrate in Fused Filament Fabrication Process
Authors: Badarinath Rakshith , Prabhu Vittaldas
Published: January 2022

In this paper we addressed key challenges in engineering an instrumentation system for sensing and signal processing for real-time estimation of two main process variables in the Fused-Filament-Fabrication process: (i) temperature of the polymer melt exiting the nozzle using a thermocouple; and (ii) polymer flowrate using extrusion width measurements in real-time, in-situ, using a microscope camera. We used a design of experiments approach to develop response surface models for two materials that enable accurate estimation of the polymer exit temperature as a function of polymer flowrate and liquefier temperature with a fit of R2=99.96% and 99.39%. The live video stream of the deposition process was used to compute the flowrate based on a road geometry model. Specifically, a robust extrusion width recognizer REXR algorithm was developed to identify edges of the deposited road and for real-time computation of extrusion width, which was found to be robust to filament colors and materials. The extrusion width measurement was found to be within 0.08 mm of caliper measurements with an R2 value of 99.91% and was found to closely track the requested flowrate from the slicer. This opens new avenues for advancing the engineering science for process monitoring and control of FFF.