bluuebunny/crossref_metadata_2025 huggingface dataset:
Cryoprotectant-Mediated Cold Stress Mitigation in Litchi Flower Development: Transcriptomic and Metabolomic Perspectives
Authors: Zheng Xue-Wen , Cao Xin-Yue , Jiang Wen-Hao , Xu Guang-Zhao , Liang Qing-Zhi , Yang Zhuan-Ying
Published: April 2024

Temperature is vital in plant growth and agricultural fruit production. Litchi chinensis Sonn, commonly known as litchi, is appreciated for its delicious fruit and fragrant blossoms and is susceptible to stress when exposed to low temperatures. This study investigates the effect of two cryoprotectants that counteract cold stress during litchi flowering, identifies the genes that generate the cold resistance induced by the treatments, and hypothesizes the roles of these genes in cold resistance. Whole plants were treated with Bihu and Liangli cryoprotectant solutions to protect inflorescences below 10 °C. The soluble protein, sugar, fructose, sucrose, glucose, and proline contents were measured during inflorescence. Sucrose synthetase, sucrose phosphate synthetase, antioxidant enzymes (SOD, POD, CAT), and MDA were also monitored throughout the flowering stage. Differentially expressed genes (DEGs), gene ontology, and associated KEGG pathways in the transcriptomics study were investigated. There were 1243 DEGs expressed after Bihu treatment and 1340 in the control samples. Signal transduction pathways were associated with 39 genes in the control group and 43 genes in the Bihu treatment group. The discovery of these genes may contribute to further research on cold resistance mechanisms in litchi. The Bihu treatment was related to 422 low-temperature-sensitive differentially accumulated metabolites (DAMs), as opposed to 408 DAMs in the control, mostly associated with lipid metabolism, organic oxidants, and alcohols. Among them, the most significant differentially accumulated metabolites were involved in pathways such as β-alanine metabolism, polycyclic aromatic hydrocarbon biosynthesis, linoleic acid metabolism, and histidine metabolism. These results showed that Bihu treatment could potentially promote these favorable traits and increase fruit productivity compared to the Liangli and control treatments. More genomic research into cold stress is needed to support the findings of this study.
On reactive Ion Etching of Parylene-C with Simple Photoresist Mask for Fabrication of High Porosity Membranes to Capture Circulating and Exfoliated Tumor Cells
Authors: Rabadi Inad , Carpentieri David , Wang Jue , Zenhausern Frederic , Gu Jian
Published: April 2024

A high porosity micropore arrayed parylene membrane is a promising device that is used to capture circulating and exfoliated tumor cells (CTCs and ETCs) for liquid biopsy applications. However, its fabrication still requires either expensive equipment or an expensive process. Here, we report on the fabrication of high porosity (>40%) micropore arrayed parylene membranes through a simple reactive ion etching (RIE) that uses photoresist as the etching mask. Vertical sidewalls were observed in etched parylene pores despite the sloped photoresist mask sidewalls, which was found to be due to the simultaneous high DC-bias RIE induced photoresist melting and substrate pedestal formation. A theoretical model has been derived to illustrate the dependence of the maximum membrane thickness on the final pore-to-pore spacing, and it is consistent with the experimental data. A simple, yet accurate, low number (<50) cell counting method was demonstrated through counting cells directly inside a pipette tip under phase-contrast microscope. Membranes as thin as 3 μm showed utility for low number tumor cell capture, with an efficiency of 87–92%.
Chiral Materials for Optics and Electronics: Ready to Rise?
Authors: Ham Seo-Hyeon , Han Moon Jong , Kim Minkyu
Published: April 2024

Chiral materials have gained burgeoning interest in optics and electronics, beyond their classical application field of drug synthesis. In this review, we summarize the diverse chiral materials developed to date and how they have been effectively applied to optics and electronics to get an understanding and vision for the further development of chiral materials for advanced optics and electronics.
The Fast Quantification of Vitamin B12 in Milk Powder by High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry
Authors: Yang Yue , Zhou Biao , Zheng Chenyang
Published: April 2024

This study presents a new technique for determining vitamin B12 in milk powder using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). We used ultrasonics with potassium ferrocyanide and zinc acetate solutions to extract the samples. 59Co was employed as the analytical target for cyanocobalamin. It was separated using a Phenomenex Luna 5 μm C18 (250 × 4.6 mm) chromatographic column with a mobile phase consisting of 1.6 mmol/L EDTA and 0.4 mmol/L KH2PO4 in a 60% v/v methanol solution (pH = 4.0). The sample has an excellent separating degree for free cobalt and cyanocobalamin, and isocratic elution can be finished within 4.0 min. To eliminate the matrix interference due to the presence of milk powder, we applied collision mode (KED). The linear range of cyanocobalamine ranged from 1.0 μg/L to 20 μg/L, with correlation coefficients (r2) of 0.9994. The limit of detection (LOD) was 0.63 μg/kg, and the limit of quantitation (LOQ) was 2.11 μg/kg. The mean recoveries were in the range of 87.4–103.6%. The accuracy and precision of the developed method are well suited for the fast quantification of the trace vitamin B12 in milk powder.
Association between Homocysteine and Vitamin D Levels in Asymptomatic Korean Adults
Authors: Lee Yun-Ah , Kang Sung-Goo , Song Sang-Wook , Kim Se-Hong
Published: April 2024

An increased homocysteine level is a risk factor for cardiovascular disease, venous thromboembolism, cerebrovascular disease, and chronic kidney disease. In addition, vitamin D deficiency is associated with coronary artery disease and metabolic disorders. The present study included data from 1375 adults (895 men and 480 women) with a mean age of 52.62 ± 9.94 years who visited the Health Promotion Center of the University Hospital in Gyeonggi-do, Republic of Korea from January 2018 to December 2022 for routine checkups that included assessments of their homocysteine and vitamin D levels. Homocysteine levels were positively associated with age, a history of hypertension, a history of diabetes, current smoking habits, and levels of low-density lipoprotein cholesterol, creatinine, uric acid, and high-sensitivity C-reactive protein. By contrast, vitamin D levels were negatively associated with serum levels of homocysteine after adjusting for covariates (β = −0.033, p < 0.001). Additional long-term prospective studies are needed to elucidate the presence of a causal relationship between vitamin D status and serum levels of homocysteine in asymptomatic Korean adults. An intervention trial is warranted to determine whether the administration of vitamin D is helpful for the primary prevention of cardiovascular disease by lowering the homocysteine level in this population.
Effects of Lactobacillus salivarius ssp. salicinius SA-03 Supplementation on Reversing Phthalate-Induced Asthma in Mice
Authors: Lin Tien-Jen , Huang Chi-Chang , Lee Mon-Chien , Lee Yen-Peng , Huang Wen-Chung , Chuang Hsiao-Li , Wang I-Jen
Published: April 2024

Probiotics may protect against asthma. We want to investigate whether probiotics can reverse the adverse effects of phthalate exposure on asthma. We selected the female offspring of BALB/c mice, born from pregnant female mice fed with diethylhexyl phthalate (DEHP). They were continuously administrated DEHP and Lactobacillus salivarius ssp. salicinius SA-03 when they were 5 weeks old, and ovalbumin (OVA) for asthma induction started at 6 weeks for 32 days. The mice were divided into four groups (n = 6/group): 1. control group (C), 2. OVA/DEHP group (OD), 3. OVA/DEHP/probiotics low-dose group (ODP-1X), and OVA/DEHP/probiotics high-dose group (ODP-5X). We found that the administration of probiotics significantly reduced the asthma severity of the mice, as well as serum IgE and IL-5. In the ODP-5X group, the proportion of CD4+ cells in the lung was reduced, whereas IL-10 in serum and CD8+ cells in BALF were increased. In histopathology, the ODP group showed reduced infiltration of inflammatory cells, bronchial epithelial cell hyperplasia, and tracheal mucus secretion. These results might indicate that high-dose probiotics may affect anti-inflammatory cytokines and reduce asthma-relative indicators. The above results may provide evidence that high-dose probiotics supplementation might play a modulating role in DEHP causes of allergic asthma in the pediatric animal model.
Supplementation of Silymarin Alone or in Combination with Salvianolic Acids B and Puerarin Regulates Gut Microbiota and Its Metabolism to Improve High-Fat Diet-Induced NAFLD in Mice
Authors: Wang Xin , Jin Yufeng , Di Can , Zeng Yupeng , Zhou Yuqing , Chen Yu , Pan Zhijun , Li Zhongxia , Ling Wenhua
Published: April 2024

Silymarin, salvianolic acids B, and puerarin were considered healthy food agents with tremendous potential to ameliorate non-alcoholic fatty liver disease (NAFLD). However, the mechanisms by which they interact with gut microbiota to exert benefits are largely unknown. After 8 weeks of NAFLD modeling, C57BL/6J mice were randomly divided into five groups and fed a normal diet, high-fat diet (HFD), or HFD supplemented with a medium or high dose of Silybum marianum extract contained silymarin or polyherbal extract contained silymarin, salvianolic acids B, and puerarin for 16 weeks, respectively. The untargeted metabolomics and 16S rRNA sequencing were used for molecular mechanisms exploration. The intervention of silymarin and polyherbal extract significantly improved liver steatosis and recovered liver function in the mice, accompanied by an increase in probiotics like Akkermansia and Blautia, and suppressed Clostridium, which related to changes in the bile acids profile in feces and serum. Fecal microbiome transplantation confirmed that this alteration of microbiota and its metabolites were responsible for the improvement in NAFLD. The present study substantiated that alterations of the gut microbiota upon silymarin and polyherbal extract intervention have beneficial effects on HFD-induced hepatic steatosis and suggested the pivotal role of gut microbiota and its metabolites in the amelioration of NAFLD.
Gut Microbiome—How Does Two-Month Consumption of Fiber-Enriched Rolls Change Microbiome in Patients Suffering from MASLD?
Authors: Kaźmierczak-Siedlecka Karolina , Maciejewska-Markiewicz Dominika , Sykulski Maciej , Gruszczyńska Agata , Herman-Iżycka Julia , Wyleżoł Mariusz , Katarzyna Petriczko Karolina , Palma Joanna , Jakubczyk Karolina , Janda-Milczarek Katarzyna , Skonieczna-Żydecka Karolina , Stachowska Ewa
Published: April 2024

Background: The occurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing global problem which commonly affects patients with co-existing diseases/conditions, such as type 2 diabetes and dyslipidemia. The effective treatment of MASLD is still limited; however, diet plays a significant role in its management. There are multiple beneficial properties of dietary fiber, including its ability to modify the gut microbiome. Therefore, the aim of this study was to determine the effect of the consumption of fiber-enriched rolls on the gut microbiome and microbial metabolites in patients suffering from MASLD. Methods: The participants were recruited according to the inclusion criteria and were required to consume fiber-enriched rolls containing either 6 g or 12 g of fiber. There were three assessment timepoints, when the anthropometric and laboratory parameters were measured, and 16s on nanopore sequencing of the fecal microbiome was conducted. Results: Firmicutes and Bacteroidetes were the most abundant phyla in the patients living with MASLD. It was demonstrated that the amount of short-chain fatty acids (SCFAs) changed after the consumption of fiber-enriched rolls; however, this was strongly associated with both the timepoint and the type of SCFAs—acetate and butyrate. Additionally, the high-fiber diet was related to the increase in phyla diversity (p = 0.006571). Conclusions: Overall, the introduction of an appropriate amount of fiber to the diet seems to be promising for patients suffering from MASLD due to its ability to create an improvement in gut microbiome-related aspects.
Sex-Specific Effects of Dietary Factors on Sarcopenic Obesity in Korean Elderly: A Nationwide Cross-Sectional Study
Authors: Kim Soojeong , Hong Kyung Hee
Published: April 2024

This study aimed to compare the dietary factors related to sarcopenia and obesity status in 5458 elderly individuals (2391 men and 3076 women) aged ≥65 years from the Korean National Health and Nutrition Examination Survey (2016–2019). Participants were categorized into normal, sarcopenia, obesity, and sarcopenic obesity groups. Sarcopenic obesity showed a higher prevalence of diabetes and lower HDL cholesterol levels compared to obesity. Sarcopenic obesity exhibited a lower total KHEI score and lower adequacy, including meat/fish/eggs/beans, than normal or obesity. In women, sarcopenic obesity scored lower than obesity on the total KHEI, adequacy for most foods, and balance of energy intake, and lower than sarcopenia on the adequacy of breakfast and milk/milk products. Sarcopenic obesity showed no significant difference in energy intake compared to sarcopenia, and less physical activity compared to sarcopenia and obesity, with a BMI/waist circumference comparable to that of obesity. Low total KHEI scores and scores for meat/fish/eggs/beans were most closely associated with sarcopenia in men and with sarcopenic obesity in women. In conclusion, low dietary quality and inadequate protein-rich foods are possibly associated with the prevalence of sarcopenic obesity in elderly Koreans, especially in women. Adequate energy intake and dietary diversity may be effective in managing sarcopenic obesity.
Neurological Adverse Events Related to Immune Checkpoint Inhibitors: A Practical Review
Authors: Zammit François , Seront Emmanuel
Published: April 2024

The increasing use of immune checkpoint inhibitors (ICI) in cancer therapy has brought attention to their associated neurotoxicities, termed neurological immune-related adverse events (n-irAEs). Despite their relatively rare incidence, n-irAEs pose a significant risk, potentially leading to severe, long-lasting disabilities or even fatal outcomes. This narrative review aims to provide a comprehensive overview of n-irAEs, focusing on their recognition and management. The review addresses a spectrum of n-irAEs, encompassing myositis, myasthenia gravis, various neuropathies, and central nervous system complications, such as encephalitis, meningitis, and demyelinating diseases. The key features of n-irAEs are emphasized in this review, including their early onset after initiation of ICIs, potential association with non-neurological irAEs and/or concurrent oncological response, the significance of ruling out other etiologies, and the expected improvement upon discontinuation of ICIs and/or immunosuppression. Furthermore, this review delves into considerations for ICI re-challenge and the intricate nature of n-irAEs within the context of pre-existing autoimmune and paraneoplastic syndromes. It underscores the importance of a multidisciplinary approach to diagnosis and treatment, highlighting the pivotal role of severity grading in guiding treatment decisions.
Effects of Ganjianglingzhu Decoction on Lean Non-Alcoholic Fatty Liver Disease in Mice Based on Untargeted Metabolomics
Authors: Tang Nan , Ji Lei , Shi Xinyu , Xiong Yalan , Xiong Xinying , Zhao Hanhua , Song Hualing , Wang Jianying , Zhang Lei , You Shengfu , Ji Guang , Liu Baocheng , Wu Na
Published: April 2024

Non-alcoholic fatty liver disease (NAFLD) is usually associated with obesity. However, it is crucial to recognize that NAFLD can also occur in lean individuals, which is frequently overlooked. Without an approved pharmacological therapy for lean NAFLD, we aimed to investigate whether the Ganjianglingzhu (GJLZ) decoction, a representative traditional Chinese medicine (TCM), protects against lean NAFLD and explore the potential mechanism underlying these protective effects. The mouse model of lean NAFLD was established with a methionine-choline-deficient (MCD) diet in male C57BL/6 mice to be compared with the control group fed the methionine-choline-sufficient (MCS) diet. After four weeks, physiological saline, a low dose of GJLZ decoction (GL), or a high dose of GJLZ decoction (GH) was administered daily by gavage to the MCD group; the MCS group was given physiological saline by gavage. Untargeted metabolomics techniques were used to explore further the potential mechanism of the effects of GJLZ on lean NAFLD. Different doses of GJLZ decoction were able to ameliorate steatosis, inflammation, fibrosis, and oxidative stress in the liver; GL performed a better effect on lean NAFLD. In addition, 78 candidate differential metabolites were screened and identified. Combined with metabolite pathway enrichment analysis, GL was capable of regulating the glucose and lipid metabolite pathway in lean NAFLD and regulating the glycerophospholipid metabolism by altering the levels of sn-3-O-(geranylgeranyl)glycerol 1-phosphate and lysoPC(P-18:0/0:0). GJLZ may protect against the development of lean NAFLD by regulating glucose and lipid metabolism, inhibiting the levels of sn-3-O-(geranylgeranyl)glycerol 1-phosphate and lysoPC(P-18:0/0:0) in glycerophospholipid metabolism.
Budesonide Attains Its Wide Clinical Profile by Alternative Kinetics
Authors: Brattsand Ralph , Selroos Olof
Published: April 2024

The introduction of inhaled corticosteroids (ICSs) changed over a few decades the treatment focus of mild-to-moderate asthma from bronchodilation to reduction in inflammation. This was achieved by inhaling a suitable corticosteroid (CS), giving a high, protracted airway concentration at a low total dose, thereby better combining efficacy and tolerance than oral therapy. Successful trials with the potent, lipophilic “skin” CS beclomethasone dipropionate (BDP) paved the way, suggesting that ICSs require a very low water solubility, prolonging their intraluminal dissolution within airways. The subsequent ICS development, with resulting clinical landmarks, is exemplified here with budesonide (BUD), showing that a similar efficacy/safety relationship is achievable by partly alternative mechanisms. BUD is much less lipophilic, giving it a 100-fold higher water solubility than BDP and later developed ICSs, leading to its more rapid intraluminal dissolution and faster airway and systemic uptake rates. In airway tissue, a BUD fraction is reversibly esterified to intracellular fatty acids, a lipophilic conjugate, which prolongs airway efficacy. Another mechanism is that the rapidly absorbed bulk fraction, via short plasma peaks, adds anti-inflammatory activity at the blood and bone marrow levels. Importantly, these plasma peaks are too short to provoke systemic adverse actions. Controlled clinical trials with BUD changed the use of ICS from a last resort to first-line treatment. Starting ICS treatment immediately after diagnosis (“early intervention”) became a landmark for BUD. An established dose response made BUD suitable for the treatment of patients with all degrees of asthma severity. With the development of the budesonide/formoterol combination inhaler (BUD/FORM), BUD contributed to the widely used BUD/FORM maintenance and reliever therapy (MART). Recent studies demonstrated the value of BUD/FORM as a generally recommended as-needed therapy for asthma (“anti-inflammatory reliever”, AIR). These abovementioned qualities have all influenced international asthma management and treatment guidelines.
Development and Validation of Novel HPLC Methods for Quantitative Determination of Vitamin D3 in Tablet Dosage Form
Authors: Gohar Muhammad Saqib , Rahman Taj Ur , Bahadur Ali , Ali Ashraf , Alharthi Sarah , Al-Shaalan Nora Hamad
Published: April 2024

In the present work, an efficient isocratic HPLC method was developed for the precise and accurate estimation of vitamin D3 in tablet form. The chromatographic conditions comprised an L3 silica column (5 µm in particle size, 4.6 mm × 250 mm) with a mobile phase n-hexane/ethyl acetate (85:15 v/v) with a flow rate of 2.0 mL/min and a detection wavelength of 292 nm. The new methodology was validated for accuracy, precision, specificity, robustness, and quantification limits according to an official monograph of USP/BP and ICH guidelines. The peak areas of the six replicates of the homogeneous sample were recorded. The mean value obtained was 67,301, and the relative standard deviation (RSD) was 0.1741. The linearity and range were in the acceptable bounds, i.e., 0.999, which was calculated using regression line analysis. The results show that the method is truly acceptable as the RSD, as the flow rate was 0.81%, while for the mobile phase composition, it was 0.72%, which lies in the acceptable range. The limit of detection (LOD) and the limit of quantification (LOQ) values were 0.0539 µg/mL and 0.1633 µg/mL, respectively. The % RSD of the intra and inter-day precision of the method was deemed acceptable according to the international commission for harmonization guidelines. The developed method has potential to be used for the detection and quantification of vitamin D3 during routine analysis for tablets in dosage form.
Aptamers for the Delivery of Plant-Based Compounds: A Review
Authors: Gamboa Joana , Lourenço Pedro , Cruz Carla , Gallardo Eugenia
Published: April 2024

Natural compounds have a high potential for the treatment of various conditions, including infections, inflammatory diseases, and cancer. However, they usually present poor pharmacokinetics, low specificity, and even toxicity, which limits their use. Therefore, targeted drug delivery systems, typically composed of a carrier and a targeting ligand, can enhance natural product selectivity and effectiveness. Notably, aptamers—short RNA or single-stranded DNA molecules—have gained attention as promising ligands in targeted drug delivery since they are simple to synthesize and modify, and they present high tissue permeability, stability, and a wide array of available targets. The combination of natural products, namely plant-based compounds, with a drug delivery system utilizing aptamers as targeting agents represents an emerging strategy that has the potential to broaden its applications. This review discusses the potential of aptamers as targeting agents in the delivery of natural compounds, as well as new trends and developments in their utilization in the field of medicine.
In Vivo Imaging of Acute Hindlimb Ischaemia in Rat Model: A Pre-Clinical PET Study
Authors: Farkasinszky Gergely , Péliné Judit Szabó , Károlyi Péter , Rácz Szilvia , Dénes Noémi , Papp Tamás , Király József , Szabo Zsuzsanna , Kertész István , Mező Gábor , Halmos Gabor , Képes Zita , Trencsényi György
Published: April 2024

Background: To better understand ischaemia-related molecular alterations, temporal changes in angiogenic Aminopeptidase N (APN/CD13) expression and glucose metabolism were assessed with PET using a rat model of peripheral arterial disease (PAD). Methods: The mechanical occlusion of the base of the left hindlimb triggered using a tourniquet was applied to establish the ischaemia/reperfusion injury model in Fischer-344 rats. 2-[18F]FDG and [68Ga]Ga-NOTA-c(NGR) PET imaging performed 1, 3, 5, 7, and 10 days post-ischaemia induction was followed by Western blotting and immunohistochemical staining for APN/CD13 in ischaemic and control muscle tissue extracts. Results: Due to a cellular adaptation to hypoxia, a gradual increase in [68Ga]Ga-NOTA-c(NGR) and 2-[18F]FDG uptake was observed from post-intervention day 1 to 7 in the ischaemic hindlimbs, which was followed by a drop on day 10. Conforming pronounced angiogenic recovery, the NGR accretion of the ischaemic extremities differed significantly from the controls 5, 7, and 10 days after ischaemia induction (p ≤ 0.05), which correlated with the Western blot and immunohistochemical results. No remarkable radioactivity was depicted between the normally perfused hindlimbs of either the ischaemic or the control groups. Conclusions: The PET-based longitudinal assessment of angiogenesis-associated APN/CD13 expression and glucose metabolism during ischaemia may continue to broaden our knowledge on the pathophysiology of PAD.
Weak Faraday Effect Measurement in Anti-Resonant Fiber Based on Intermodal Interference Suppression
Authors: Guo Zhiyong , Du Haoqi , Lin Yuhao , Yu Zhangjun
Published: April 2024

Anti-resonant fiber (ARF) works well in a relatively strong magnetic field due to its weak Faraday effect, which results from the fundamental mode mainly transmitting in the air core. Accurately measuring the Faraday effect strength, i.e., the effective Verdet constant, of an ARF determines its applicable scenarios. However, the effective Verdet constant of ARF is ~3 orders of magnitude lower than that of a standard single-mode fiber, which is very difficult to measure. In this paper, we reveal that intermodal interference is the main obstacle to measuring the ultralow effective Verdet constant of ARF and propose using a narrow-band low-coherence light to suppress it. The measured effective Verdet constant of ARF is 0.423 ± 0.005 mrad/T/m at 1550 nm.
Continuous-Variable Measurement-Device-Independent Quantum Key Distribution in the Terahertz Band
Authors: Liu Haoxin , Yang Zhe , Yang Shuoyu , Sun Dongyun , Zhang Cunlin
Published: April 2024

We have introduced, for the first time, a protocol for Continuous-Variable Measurement-Device-Independent Quantum Key Distribution (CV-MDI-QKD) in the terahertz (THz) frequency band. We have conducted a secret key rate analysis against collective attacks. The proposed THz CV-MDI-QKD is immune to all detector attacks, significantly enhancing the security assurance of practical THz CVQKD implementations. Furthermore, we investigated the impact of finite key length (the finite-size effect) and finite reconciliation efficiency on the performance of practical THz CV-MDI-QKD systems. Our findings reveal that by employing a large number of keys or signals and optimizing the modulation variance, the detrimental effects arising from the finite-size effect and suboptimal reconciliation efficiency can be notably mitigated. These insights play a crucial role in advancing the feasibility of THz CVQKD technology towards practical applications.
Removal of Erythromycin from Water by Ibuprofen-Driven Pre-Organized Divinyl Sulfone Cross-Linked Dextrin
Authors: Ortega-Muñoz Mariano , Alvarado Sarah , Megia-Fernandez Alicia , Hernandez-Mateo Fernando , Lopez-Jaramillo Francisco Javier , Santoyo-Gonzalez Francisco
Published: April 2024

Water recycling and reuse are cornerstones of water management, which can be compromised by the presence of pollutants. Among these, pharmaceuticals can overcome standard water treatments and require sophisticated approaches to remove them. Sorption is an economically viable alternative limited by the need for sorbents with a sorption coefficient (Kd) higher than 500 L/kg. The cross-linking of dextrin (Dx) with divinyl sulfone (DVS) in the presence of 1 mmol or 5 mmol of ibuprofen (IBU) yields the insoluble polymers pDx1 and pDx5 with improved affinity for IBU and high selectivity towards erythromycin (ERY) and ERY Kd higher than 4 × 103 L/kg, when tested against a cocktail of six drugs. Characterization of the polymers shows that both pDx1 and pDx5 have similar properties, fast sorption kinetics, and ERY Kd of 13.3 × 103 for pDx1 and 6.4 × 103 for pDx5, representing 26.6 and 12.0 times the 500 L/kg threshold. The fact that new affinities and improvements in Kd can be achieved by cross-linking Dx in the presence of other molecules that promote pre-organization expands the applications of DVS cross-linked polysaccharides as sustainable, scalable, and environmentally friendly sorbents with a potential application in wastewater treatment plants (WTPs).
Analysis on Isotropic and Anisotropic Samples of Polypropylene/Polyethyleneterephthalate Blend/Graphene Nanoplatelets Nanocomposites: Effects of a Rubbery Compatibilizer
Authors: Titone Vincenzo , Baiamonte Marilena , Ceraulo Manuela , Botta Luigi , Mantia Francesco Paolo La
Published: April 2024

Over the past few years, polymer nanocomposites have garnered a significant amount of interest from both the scientific community and industry due to their remarkable versatility and wide range of potential uses in various fields, including automotive, electronics, medicine, textiles and environmental applications. In this regard, this study focuses on the influence of a compatibilizer rubber on a nanocomposite incorporating graphene nanoparticles (GNPs), with a polymer matrix based on a blend of polypropylene (PP) and polyethylene terephthalate (PET). This effect has been investigated on both isotropic samples and on anisotropic/spun fiber samples. The influence of the compatibilizer rubber on morphological, rheological and mechanical properties was analysed and discussed. Mechanical and morphological properties were evaluated on both isotropic samples obtained by compression moulding and melt-spun fibers. The addition of the rubbery compatibilizer increased the viscosity, improving interfacial adhesion, and the same effect was observed for the melt strength and breaking stretching ratios. Mechanical properties, including the elastic modulus, tensile strength and elongation at break, improved in both types of samples but more significantly in the fibers. These improvements were attributed to the orientation of the matrix, the formation of PET microfibrils, and the reduction in the size of graphene nanoparticles due to the action of the elongational flow. This reduction, facilitated by the elongation flow and the action of the compatibilizer, improved matrix–nanofiller adhesion due to the increased contact area between the two polymeric phases and between the filler and matrix. Finally, a transition from brittle to ductile behaviour was observed, particularly in the system with the compatibilizer, attributed to defect reduction and improved stress transmission.
Nanocellulose Grades with Different Morphologies and Surface Modification as Additives for Waterborne Epoxy Coatings
Authors: Samyn Pieter , Cosemans Patrick
Published: April 2024

While adding different micro- and nanocellulose types into epoxy coating formulations with waterborne phenalkamine crosslinker, effects on processing conditions and coating performance were systematically investigated. The variations in viscosity, thermal and thermomechanical properties, mechanical behavior, abrasive wear, water contact angles, and coating morphologies were evaluated. The selected additives include microcrystalline cellulose (MCC) at 1 to 10 wt.% and cellulose nanocrystals (CNC), cellulose nanofibers (CNF), cellulose microfibers (CMF), and hydrophobically modified cellulose microfibers (mCMF) at 0.1 to 1.5 wt.%. The viscosity profiles are determined by the inherent additive characteristics with strong shear thinning effects for epoxy/CNF, while the epoxy/mCMF provides lower viscosity and better matrix compatibility owing to the lubrication of encapsulated wax. The crosslinking of epoxy/CNF is favored and postponed for epoxy/(CNC, CMF, mCMF), as the stronger interactions between epoxy and CNF are confirmed by an increase in the glass transition temperature and reduction in the dampening factor. The mechanical properties indicate the highest hardness and impact strength for epoxy/CNF resulting in the lowest abrasion wear rates, but ductility enhances and wear rates mostly reduce for epoxy/mCMF together with hydrophobic protection. In addition, the mechanical reinforcement owing to the specific organization of a nanocellulose network at percolation threshold concentrations of 0.75 wt.% is confirmed by microscopic analysis: the latter results in a 2.6 °C (CNF) or 1.6 °C (CNC) increase in the glass transition temperature, 50% (CNF) or 20% (CNC) increase in the E modulus, 37% (CNF) or 32% (CNC) increase in hardness, and 58% (CNF) or 33% (CNC) lower abrasive wear compared to neat epoxy, while higher concentrations up to 1.5 wt.% mCMF can be added. This research significantly demonstrates that nanocellulose is directly compatible with a waterborne phenalkamine crosslinker and actively contributes to the crosslinking of waterborne epoxy coatings, changing the intrinsic glass transition temperatures and hardness properties, to which mechanical coating performance directly relates.
Enhancing Fatigue Performance of Coal Gangue Concrete (CGC) through Polypropylene Fiber Modification: Experimental Evaluation
Authors: Wu Di , Jing Laiwang , Li Yan , Ran Tao , Peng Shaochi , Jing Wei
Published: April 2024

Coal gangue is a byproduct of coal mining and processing, and according to incomplete statistics, China has amassed a substantial coal gangue stockpile exceeding 2600 large mountains, which poses a serious threat to the ecological environment. Utilizing gangue as a coarse aggregate to produce gangue concrete (GC) presents a promising avenue for addressing the disposal of coal gangue; however, gangue concrete presents several challenges that need to be tackled, such as low strength and poor resistance to repeated loads. In this study, polypropylene fibers (PPFs) were incorporated into gangue concrete to enhance its utilization rate. Uniaxial compressive and repeated loading experiments were then conducted to investigate the uniaxial strength and fatigue properties of polypropylene fiber-reinforced gangue concrete (PGC) with varying gangue substitution rates (20%, 40%, and 60%) and different polypropylene fiber admixtures (0, 0.1%, 0.2%, and 0.3%). The findings indicate that incorporating gangue at a substitution rate of 40% could notably enhance the uniaxial compressive strength of PGC, resulting in a maximum increase of 19.4%. In the repeated loading experiments, the ductility of PGC was enhanced with the incorporation of PPFs, resulting in a reduction of 33.76% in the damage factor and 19.42% in residual strain for PGC-40-0.2 compared to PGC-40-0. A PPF content of 0.2% was found to be optimal for enhancing the fatigue performance of PGC. Scanning electron microscope (SEM) testing proved the improvement effect of polypropylene fiber on gangue concrete from a microscopic perspective. This study provides crucial experimental data and a theoretical foundation for the utilization of gangue concrete in complex stress environments.
A New Approach to Implant Stability Using a Flexible Synthetic Silicate-Additive Beta-Tricalcium Phosphate-Poly(D,L-lactide-co-caprolactone) Bone Graft: An In Vitro Study
Authors: Orhan Zeynep Dilan , Ciğerim Levent
Published: April 2024

The aim of this study was to evaluate the use of a flexible synthetic polymer bone graft to provide implant stability during implant placement in a dense cortical bone model. In the control group (Group 1), sockets were prepared on polyurethane blocks according to the standard implant socket drilling protocol; both oversizing and deepening were applied in Group 2; and only oversizing was applied in Group 3. In Groups 2 and 3, flexible synthetic polymer bone grafts were placed in the sockets prior to implant placement. The implants were placed at the bone level in all groups. The highest torque value obtained was recorded as the insertion torque. In this study, 75 implant sites were included across three groups. The torque values of the implants in the control group were significantly higher than those of the implants with the oversized and deepened sockets and the oversized-only sockets (p < 0.05; p < 0.01). The torque values of the implants with the oversized and deepened sockets were significantly higher than those of the implants with the oversized-only sockets (p < 0.01). In this study, a flexible synthetic polymer bone graft was shown to be effective in achieving implant stability in the management of implants where there has been a loss of primary stability.
Application of ChatGPT in Information Literacy Instructional Design
Authors: Madunić Jelena , Sovulj Matija
Published: April 2024

Recent developments in generative artificial intelligence tools have prompted immediate reactions in the academic library community. While most studies focus on the potential impact on academic integrity, this work explored constructive applications of ChatGPT in the design of instructional materials for courses in academic information literacy. The starting point was the use of openly licenced information resources or content infrastructure as facilitators in the creation of educational materials. In the first phase, course teaching material was developed using a prompt engineering strategy, predefined standards, and a prompt script. As a second step, we experimented with designing a custom chatbot model connected to a pre-defined corpus of source documents. The results demonstrated that the final teaching material required careful revision and optimisation before use in an actual instructional programme. The experimental design of the custom chatbot was able to query specific user-defined documents. Taken together, these findings suggest that the strategic and well-planned use of ChatGPT technology in content creation can have substantial benefits in terms of time and cost efficiency. In the context of information literacy, the results provide a practical and innovative solution to integrate the new technology tool into instructional practices.
Two-Dimensional Target Localization Approach via a Closed-Form Solution Using Range Difference Measurements Based on Pentagram Array
Authors: Khalafalla Mohammed , Jiang Kaili , Tian Kailun , Feng Hancong , Xiong Ying , Tang Bin
Published: April 2024

This paper presents a simple and fast closed-form solution approach for two-dimensional (2D) target localization using range difference (RD) measurements. The formulation of the localization problem is derived using a pentagram array. The target position is determined using passive radar measurements (RDs) between the target and the (N+1=10) receivers’ locations. The method facilitates the problem of target position and can be used as a counter-parallel method for spherical interpolation (SI) and spherical intersection (SX) methods in time difference of arrival (TDOA) and radar systems. The performance of the method is examined in 2D target localization using numerical analysis under the distribution of receivers in the pentagram array. The simulations are conducted using four different far-distance targets and comparatively large-area distributed receivers. The RD measurements were distorted by two different values of Gaussian errors based on ionosphedriec time delays of 20 and 50 nsec owing to the different receivers’ positions. The findings highly verified the validity of the method for addressing the problem of target localization. Additionally, a theoretical accuracy study of the method is given, which solely relies on the RD measurements.
Forest Community Spatial Modeling Using Machine Learning and Remote Sensing Data
Authors: Gafurov Artur , Prokhorov Vadim , Kozhevnikova Maria , Usmanov Bulat
Published: April 2024

This study examines the application of unsupervised classification techniques in the mapping of forest vegetation, aiming to align vegetation cover with the Braun-Blanquet classification system through remote sensing. By leveraging Landsat 8 and 9 satellite imagery and advanced clustering algorithms, specifically the Weka X-Means, this research addresses the challenge of minimizing researcher subjectivity in vegetation mapping. The methodology incorporates a two-step clustering approach to accurately classify forest communities, utilizing a comprehensive set of vegetation indices to distinguish between different types of forest ecosystems. The validation of the classification model relied on a detailed analysis of over 17,000 relevés from the “Flora” database, ensuring a high degree of accuracy in matching satellite-derived vegetation classes with field observations. The study’s findings reveal the successful identification of 44 forest community types that was aggregated into seven classes of Braun-Blanquet classification system, demonstrating the efficacy of unsupervised classification in generating reliable vegetation maps. This work not only contributes to the advancement of remote sensing applications in ecological research, but also provides a valuable tool for natural resource management and conservation planning. The integration of unsupervised classification with the Braun-Blanquet system presents a novel approach to vegetation mapping, offering insights into ecological characteristics, and can be good starter point for sequestration potential of forest communities’ assessment in the Republic of Tatarstan.
Range-Doppler-Time Tensor Processing for Deep-Space Satellite Characterization Using Narrowband Radar
Authors: Serrano Alexander , Capper Jack , Morrison Robert L. , Abouzahra Mohamed D.
Published: April 2024

There is growing demand for the high-fidelity characterization of satellites in Geosynchronous Earth Orbit (GEO) to support Space Domain Awareness (SDA). This is particularly true for newly launched satellites, where it is necessary for satellite providers to ascertain whether components have deployed properly. Conventional wideband radar systems are capable of imaging satellites provided that (i) they have sufficient power aperture and bandwidth, and (ii) they observe enough target aspect change to generate a resolved image. While wideband radars are used routinely for characterizing satellites in Low-Earth Orbit (LEO), powerful radars with sensitivity sufficient for large GEO ranges (36,000 km or greater) are lacking. Thus, researchers often rely on more widely available high-power narrowband tracking radars for GEO characterization. In this paper, we present a novel range-Doppler-time (RDT) tensor processing technique for GEO characterization with narrowband radar. This technique encapsulates the strengths of previously proposed methods for narrowband-radar characterization at GEO, providing a generalized approach that can be applied in a variety of settings. The technique generates fully resolved 2D images of rotating GEO satellites in low-bandwidth scenarios. In cases where aspect change is limited, the technique provides detailed Doppler information for enhanced satellite status monitoring. This work presents a comprehensive quantitative analysis of the technique that considers the impact of key parameters on characterization performance. Simulated radar data, and radar data collected in a compact range on a scaled satellite model, are used to evaluate the technique.
Urban Growth and Its Ecological Effects in China
Authors: Chen Hanqian , Deng Shuyu , Zhang Shunxue , Shen Yao
Published: April 2024

As the largest developing nation, China is currently experiencing rapid urban growth. Conducting a thorough scientific assessment of this expansion and its ecological consequences is of paramount importance for advancing China’s ecological civilization and aligning with the United Nations’ Sustainable Development Goals. This study employs multi-source remote sensing data to investigate the spatiotemporal trends in Chinese urban development and explore its impact on the ecological environment. From 2013 to 2021, the findings indicate an increasing trend in China’s total nocturnal light, with the southern and central regions exhibiting higher growth rates. This suggests a decade-long expansion of Chinese cities, especially in the southern and central regions. However, the impact of urban expansion on ecological quality varies. Beijing, Shenyang, and Xi’an have witnessed improved environmental quality, while Kunming and Shenzhen have experienced minimal changes, and Hefei and Wuhan have encountered a decline. The observed spatial heterogeneity underscores the intricate relationship between urban expansion and ecological shifts. This study reveals the spatiotemporal dynamics of China’s urban expansion and its ecological impact, providing valuable insights and policy recommendations for fostering the harmonized development of urbanization and ecological preservation.
Crop Canopy Nitrogen Estimation from Mixed Pixels in Agricultural Lands Using Imaging Spectroscopy
Authors: Jamalinia Elahe , Dai Jie , Vaughn Nicholas R. , Martin Roberta E. , Hondula Kelly , König Marcel , Heckler Joseph , Asner Gregory P.
Published: April 2024

Accurate retrieval of canopy nutrient content has been made possible using visible-to-shortwave infrared (VSWIR) imaging spectroscopy. While this strategy has often been tested on closed green plant canopies, little is known about how nutrient content estimates perform when applied to pixels not dominated by photosynthetic vegetation (PV). In such cases, contributions of bare soil (BS) and non-photosynthetic vegetation (NPV), may significantly and nonlinearly reduce the spectral features relied upon for nutrient content retrieval. We attempted to define the loss of prediction accuracy under reduced PV fractional cover levels. To do so, we utilized VSWIR imaging spectroscopy data from the Global Airborne Observatory (GAO) and a large collection of lab-calibrated field samples of nitrogen (N) content collected across numerous crop species grown in several farming regions of the United States. Fractional cover values of PV, NPV, and BS were estimated from the GAO data using the Automated Monte Carlo Unmixing algorithm (AutoMCU). Errors in prediction from a partial least squares N model applied to the spectral data were examined in relation to the fractional cover of the unmixed components. We found that the most important factor in the accuracy of the partial least squares regression (PLSR) model is the fraction of photosynthetic vegetation (PV) cover, with pixels greater than 60% cover performing at the optimal level, where the coefficient of determination (R2) peaks to 0.66 for PV fractions of more than 60% and bare soil (BS) fractions of less than 20%. Our findings guide future spaceborne imaging spectroscopy missions as applied to agricultural cropland N monitoring.