bluuebunny/crossref_metadata_2025 huggingface dataset:
GLOBAL EVIL, TECHNOLOGY, AND THE ALIENATION OF REALITY IN WILLIAM GIBSON’S NEUROMANCER (1984)
Authors: Giacomolli Diorgi
Published: September 2024

This article delves into the intricate relationship between technology and reality within the context of William Gibson’s seminal cyberpunk novel Neuromancer (1984) to verify how the characters’ humanity deteriorates due to the information era’s evil influence. Divided into five sections, the article unfolds by providing an introduction to William Gibson’s work, emphasizing the relevance of investigating technology’s impact on shaping reality in the contemporary era. The second section contextualizes the novel within the socio-cultural and technological landscape of the 1980s, tracing the emergence of cyberpunk as a genre and highlighting key events influencing the narrative. The third section comprises significant concepts from works by Jean Baudrillard, Susan Neiman, Ray Kurzweil, Shoshana Zuboff, Mark O’Connell, among others, establishing a theoretical framework for understanding the intertwining of technology and reality. Finally, the fourth section scrutinizes Neuromancer (1984) through this theoretical lens, examining specific passages that illustrate the novel’s dystopian vision of a world dominated by high technology and populated by a marginalized society. The analysis probes the virtual world, the blurring of reality, and the implications of technology on identity and agency. In conclusion, the article synthesizes key findings, reflecting on the implications for comprehending the contemporary intersection of technology and reality.
Latest clinical research
Authors: Greener Mark
Published: September 2024

The latest studies of interest to primary care clinicians, rounded up by Mark Greener
Career profile
Authors: Barnacle Elizabeth
Published: September 2024

Elizabeth Barnacle, Head of safeguarding, Spectrum Community Health CIC
The complex interplay between mechanical forces, tissue response and individual susceptibility to pressure ulcers
Authors: Gefen Amit
Published: September 2024

Objective:

The most recent edition of the International Clinical Practice Guideline for the Prevention and Treatment of Pressure Ulcers/Injuries was released in 2019. Shortly after, in 2020, the first edition of the SECURE Prevention expert panel report, focusing on device-related pressure ulcers/injuries, was published as a special issue in the Journal of Wound Care. A second edition followed in 2022. This article presents a comprehensive summary of the current understanding of the causes of pressure ulcers/injuries (PU/Is) as detailed in these globally recognised consensus documents.

Method:

The literature reviewed in this summary specifically addresses the impact of prolonged soft tissue deformations on the viability of cells and tissues in the context of PU/Is related to bodyweight or medical devices.

Results:

Prolonged soft tissue deformations initially result in cell death and tissue damage on a microscopic scale, potentially leading to development of clinical PU/Is over time. That is, localised high tissue deformations or mechanical stress concentrations can cause microscopic damage within minutes, but it may take several hours of continued mechanical loading for this initial cell and tissue damage to become visible and clinically noticeable. Superficial tissue damage primarily stems from excessive shear loading on fragile or vulnerable skin. In contrast, deeper PU/Is, known as deep tissue injuries, typically arise from stress concentrations in soft tissues at body regions over sharp or curved bony prominences, or under stiff medical devices in prolonged contact with the skin.

Conclusion:

This review promotes deeper understanding of the pathophysiology of PU/Is, indicating that their primary prevention should focus on alleviating the exposure of cells and tissues to stress concentrations. This goal can be achieved either by reducing the intensity of stress concentrations in soft tissues, or by decreasing the exposure time of soft tissues to such stress concentrations.
Innovative air mattress for the prevention of pressure ulcers in neonates
Authors: Jucker Tino Adrian , Annaheim Simon , Morlec Elodie , Camenzind Martin , Schlüer Anna-Barbara , Brotschi Barbara , Rossi René Michel
Published: September 2024

Objective:

Pressure ulcers (PUs) severely impact health outcomes in neonatal intensive care, with up to 28% prevalence and doubled mortality rates. Due to their only partially developed stratum corneum, neonates are highly susceptible to PUs because of a lack of adequate support surfaces. The occipital region of the head and hip are the main risk areas due to immobility and newborn body proportions. The main goal of the study was to investigate the impact of reduction in local pressure in these body areas by two air mattress designs and different filling states.

Method:

Two innovative air-filled mattress prototypes (prototype 1 and prototype 2), consisting of three different segments (head, trunk and feet regions), were developed to reduce local interface pressures by optimising pressure distribution, and were assessed with three air pressure filling states (0.2kPa, 0.4kPa and 0.6kPa). A baby doll was used to investigate pressure distribution and local pressure impact. It measured 51cm and the weight was modified to be 1.3kg, 2.3kg and 3.3kg, representing premature to term newborn weights, respectively. A specialised foam mattress and an unsupported surface were considered as controls.

Results:

The interface pressures at the hip region for newborn models could be reduced by up to 41% with mattress prototype 1 and 49% with prototype 2 when filled with 0.2kPa air pressure. It was found that the size and the pressure inside air segments was crucial for interface pressure.

Conclusion:

Our results demonstrated that air mattresses achieved lower interface pressures compared to conventional support surfaces, and that the benefit of the air mattresses depended on their filling status. The importance of using innovative, segmented designs that were tailored to meet the specific needs of highly vulnerable paediatric patients was demonstrated.
Biomarkers of local inflammation at the skin's surface may predict both pressure and diabetic foot ulcers
Authors: Wilson Pauline , Patton Declan , O'Connor Tom , Boland Fiona , Budri Aglecia MV , Moore Zena , Phelan Niamh
Published: September 2024

This commentary considers the similarities which exist between pressure ulcers (PUs) and diabetic foot ulcers (DFUs). It aims to describe what is known to be shared—both in theory and practice—by these wound types. It goes on to detail the literature surrounding the role of inflammation in both wound types. PUs occur following prolonged exposure to pressure or pressure in conjunction with shear, either due to impaired mobility or medical devices. As a result, inflammation occurs, causing cell damage. While DFUs are not associated with immobility, they are associated with altered mobility occurring as a result of complications of diabetes. The incidence and prevalence of both types of lesions are increased in the presence of multimorbidity. The prediction of either type of ulceration is challenging. Current risk assessment practices are reported to be ineffective at predicting when ulceration will occur. While systemic inflammation is easily measured, the presence of local or subclinical inflammation is harder to discern. In patients at risk of either DFUs or PUs, clinical signs and symptoms of inflammation may be masked, and systemic biomarkers of inflammation may not be elevated sufficiently to predict imminent damage until ulceration appears. The current literature suggests that the use of local biomarkers of inflammation at the skin's surface, namely oedema and temperature, may identify early tissue damage.
Genome-wide association study between SARS-CoV-2 single nucleotide polymorphisms and virus copies during infections
Authors: Li Ke , Chaguza Chrispin , Stamp Julian , Chew Yi Ting , Chen Nicholas F. G. , Ferguson David , Pandya Sameer , Kerantzas Nick , Schulz Wade ,   Yale SARS-CoV-2 Genomic Surveillance Initiative, Hahn Anne M. , Ogbunugafor C. Brandon , Pitzer Virginia E. , Crawford Lorin , Weinberger Daniel M. , Grubaugh Nathan D.
Published: September 2024

Significant variations have been observed in viral copies generated during SARS-CoV-2 infections. However, the factors that impact viral copies and infection dynamics are not fully understood, and may be inherently dependent upon different viral and host factors. Here, we conducted virus whole genome sequencing and measured viral copies using RT-qPCR from 9,902 SARS-CoV-2 infections over a 2-year period to examine the impact of virus genetic variation on changes in viral copies adjusted for host age and vaccination status. Using a genome-wide association study (GWAS) approach, we identified multiple single-nucleotide polymorphisms (SNPs) corresponding to amino acid changes in the SARS-CoV-2 genome associated with variations in viral copies. We further applied a marginal epistasis test to detect interactions among SNPs and identified multiple pairs of substitutions located in the spike gene that have non-linear effects on viral copies. We also analyzed the temporal patterns and found that SNPs associated with increased viral copies were predominantly observed in Delta and Omicron BA.2/BA.4/BA.5/XBB infections, whereas those associated with decreased viral copies were only observed in infections with Omicron BA.1 variants. Our work showcases how GWAS can be a useful tool for probing phenotypes related to SNPs in viral genomes that are worth further exploration. We argue that this approach can be used more broadly across pathogens to characterize emerging variants and monitor therapeutic interventions.
Performance of Publicly Available Large Language Models on Internal Medicine Board-style Questions
Authors: Tarabanis Constantine , Zahid Sohail , Mamalis Marios , Zhang Kevin , Kalampokis Evangelos , Jankelson Lior
Published: September 2024

Ongoing research attempts to benchmark large language models (LLM) against physicians’ fund of knowledge by assessing LLM performance on medical examinations. No prior study has assessed LLM performance on internal medicine (IM) board examination questions. Limited data exists on how knowledge supplied to the models, derived from medical texts improves LLM performance. The performance of GPT-3.5, GPT-4.0, LaMDA and Llama 2, with and without additional model input augmentation, was assessed on 240 randomly selected IM board-style questions. Questions were sourced from the Medical Knowledge Self-Assessment Program released by the American College of Physicians with each question serving as part of the LLM prompt. When available, LLMs were accessed both through their application programming interface (API) and their corresponding chatbot. Mode inputs were augmented with Harrison’s Principles of Internal Medicine using the method of Retrieval Augmented Generation. LLM-generated explanations to 25 correctly answered questions were presented in a blinded fashion alongside the MKSAP explanation to an IM board-certified physician tasked with selecting the human generated response. GPT-4.0, accessed either through Bing Chat or its API, scored 77.5–80.7% outperforming GPT-3.5, human respondents, LaMDA and Llama 2 in that order. GPT-4.0 outperformed human MKSAP users on every tested IM subject with its highest and lowest percentile scores in Infectious Disease (80th) and Rheumatology (99.7th), respectively. There is a 3.2–5.3% decrease in performance of both GPT-3.5 and GPT-4.0 when accessing the LLM through its API instead of its online chatbot. There is 4.5–7.5% increase in performance of both GPT-3.5 and GPT-4.0 accessed through their APIs after additional input augmentation. The blinded reviewer correctly identified the human generated MKSAP response in 72% of the 25-question sample set. GPT-4.0 performed best on IM board-style questions outperforming human respondents. Augmenting with domain-specific information improved performance rendering Retrieval Augmented Generation a possible technique for improving accuracy in medical examination LLM responses.
Six-year (2016–2022) longitudinal patterns of mental health service utilization rates among children developmentally vulnerable in kindergarten and the COVID-19 pandemic disruption
Authors: Talarico Fernanda , Metes Dan , Wang Mengzhe , Hayward Jake , Liu Yang S. , Tian Julie , Zhang Yanbo , Greenshaw Andrew J. , Gaskin Ashley , Janus Magdalena , Cao Bo
Published: September 2024

Introduction

In the context of the COVID-19 pandemic, it becomes important to comprehend service utilization patterns and evaluate disparities in mental health-related service access among children.

Objective

This study uses administrative health records to investigate the association between early developmental vulnerability and healthcare utilization among children in Alberta, Canada from 2016 to 2022.

Methods

Children who participated in the 2016 Early Development Instrument (EDI) assessment and were covered by public Alberta health insurance were included (N = 23 494). Linear regression models were employed to investigate the association between service utilization and vulnerability and biological sex. Separate models were used to assess vulnerability specific to each developmental domain and vulnerability across multiple domains. The service utilization was compared between pre- and post-pandemic onset periods.

Results

The analysis reveals a significant decrease in all health services utilization from 2016 to 2019, followed by an increase until 2022. Vulnerable children had, on average, more events than non-vulnerable children. There was a consistent linear increase in mental health-related utilization from 2016 to 2022, with male children consistently experiencing higher utilization rates than females, particularly among vulnerable children. Specifically, there was a consistent linear increase in the utilization of anxiety-related services by children from 2016 to 2022, with females having, on average, 25 more events than males. The utilization of ADHD-related services showed different patterns for each group, with vulnerable male children having more utilization than their peers.

Conclusion

Utilizing population-wide data, our study reveals sex specific developmental vulnerabilities and its impact on children’s mental health service utilization during the COVID-19 pandemic, contributing to the existing literature. With data from kindergarten, we emphasize the need for early and targeted intervention strategies, especially for at-risk children, offering a path to reduce the burden of childhood mental health disorders.
Trends in body mass index for people with and without HIV: Pooled analysis of nationally-representative health surveys from 10 countries and 173,800 adults in Africa
Authors: Carrillo-Larco Rodrigo M. , Bulstra Caroline A. , Manne-Goehler Jennifer , Siedner Mark J. , Johnson Leslie C. M. , Marconi Vincent C. , Chung Michael H. , Francois Venter Willem Daniel , Kocher Erica , Lalla-Edward Samanta , Chandiwana Nomathemba C. , Kariuki Jacob K. , Ali Mohammed K.
Published: September 2024

It remains unclear if and how body mass index (BMI) levels have changed over time in HIV endemic regions. We described trends in mean BMI and prevalence of overweight between 2003–2019 in 10 countries in Africa including people living with (PLWH) and without (PLWoH) HIV. We pooled Demographic and Health Surveys (DHS) from countries where ≥2 surveys >4 years apart were available with height/weight measurements and HIV tests. HIV status was ascertained with a finger-prick dried blood spot (DBS) specimen tested in a laboratory. The DBS is taken as part of the regular DHS procedures. We summarized age and socioeconomic status standardized sex-specific mean BMI (kg/m2) and prevalence of overweight (BMI ≥25 kg/m2) by HIV status. We fitted country-level meta-regressions to ascertain if changes in ART coverage were correlated with changes in BMI. Before 2011, women LWH (22.9 [95% CI: 22.2–23.6]) and LWoH (22.6 [95% CI: 22.3–22.8]) had similar mean BMI. Over time, mean BMI increased more in women LWH (+0.8 [95% CI: 0.7–0.8] BMI units) than LWoH (+0.2 [95% CI: 0.2–0.3]). Before 2013, the mean BMI was similar between men LWH (21.1 (95% CI: 20.3–21.9)) and LWoH (20.8 (95% CI: 20.6–21.1)). Over time, mean BMI increased more in men LWoH (+0.3 [95% CI: 0.3–0.3]) than LWH (+0.1 [95% CI: 0.1–0.1]). The same profile was observed for prevalence of overweight. ART coverage was not strongly associated with BMI changes. Mean BMI and prevalence of overweight were similar in PLWH and PLWoH, yet in some cases the estimates for PWLH were on track to catch up with those for PLWoH. BMI monitoring programs are warranted in PLWH to address the rising BMI trends.
Expanding the roles of malaria post workers in Thailand: A qualitative study of stakeholder perspectives
Authors: Jongdeepaisal Monnaphat , Khonputsa Panarasri , Prasert Orathai , Maneenate Supitsara , Sirimatayanant Massaya , Sopa Paradorn , Saisong Arisa , Charoensup Ittisak , Kamsri Tanong , Tipmontree Rungrawee , Sudathip Prayuth , Liverani Marco , Maude Richard J. , Pell Christopher
Published: September 2024

In Thailand, since the 2000s, malaria post (MP) workers have been tasked with promptly detecting and treating all malaria cases to prevent onward transmission in the communities. Expanding their roles to provide health services beyond malaria has been proposed as a strategy to sustain their activities until elimination is reached. This article examines the perspectives of stakeholders on community-based malaria care to assess prospects for expanding the role of MPs. The study incorporated in-depth interviews (IDIs) and focus group discussions (FGDs). In forested communities and local health facilities in northeast Thailand bordering Lao PDR and Cambodia, where malaria transmission is low, IDIs were conducted with 13 MPs and 23 community members. An additional 14 policymakers and implementers across the health sector in Thailand were interviewed. The respondents highlighted how in these border areas population groups most at risk of malaria, namely forest goers and migrants, are reluctant to visit public health facilities. In these areas, MP workers are well integrated in their communities and remain relevant although the communities no longer see malaria as spriority. Common conditions such as dengue, diabetes, insect bites, diarrhea, mental illness and substance abuse, were identified as local health concerns needing potential add-on services from MP workers. Although challenges in terms of training, supervision, and financing were raised, opportunities included additional funds from local administrative offices to maintain and integrate malaria activities with other health programmes. Changes to the roles of MPs should be adapted to changing local needs, some of which were identified in this study, should avoid duplication and potential tensions with other local health programmes, and need to build on the capacity of the community and primary care system. These enabling factors are worthy of consideration by any malaria programmes looking into maintaining their village malaria workers in the Greater Mekong Subregion.
Impact on beer sales of removing the pint serving size: An A-B-A reversal trial in pubs, bars, and restaurants in England
Authors: Mantzari Eleni , Hollands Gareth J. , Law Martin , Couturier Dominique-Laurent , Marteau Theresa M.
Published: September 2024

Background

Smaller serving sizes could contribute towards reducing alcohol consumption across populations and thereby decrease the risk of 7 cancers and other diseases. To our knowledge, the current study is the first to assess the impact on beer, lager, and cider sales (hereafter, for ease, referred to just as “beer sales”) of removing the largest draught serving size (1 imperial pint) from the options available in licensed premises under real-word conditions.

Methods and findings

The study was conducted between February and May 2023, in 13 licensed premises in England. It used an A-B-A reversal design, set over 3 consecutive 4-weekly periods with “A” representing the nonintervention periods during which standard serving sizes were served, and “B” representing the intervention period when the largest serving size of draught beer (1 imperial pint (568 ml)) was removed from existing ranges so that the largest size available was two-thirds of a pint. Where two-third pints were not served, the intervention included introducing this serving size in conjunction with removing the pint serving size. The primary outcome was the mean daily volume of all beer sold, including draught, bottles, and cans (in ml), extracted from electronic sales data. Secondary outcomes were mean daily volume of wine sold (ml) and daily revenue (£). Thirteen premises completed the study, 12 of which did so per protocol and were included in the primary analysis. After adjusting for prespecified covariates, the intervention resulted in a mean daily change of −2,769 ml (95% CI [−4,188, −1,578] p < 0.001) or −9.7% (95% CI [−13.5%, −6.1%] in beer sold. The daily volume of wine sold increased during the intervention period by 232 ml (95% CI [13, 487], p = 0.035) or 7.2% (95% CI [0.4%, 14.5%]). Daily revenues decreased by 5.0% (95% CI [9.6%, −0.3%], p = 0.038).

Conclusions

Removing the largest serving size (the imperial pint) for draught beer reduced the volume of beer sold. Given the potential of this intervention to reduce alcohol consumption, it merits consideration in alcohol control policies.

Trial registration

ISRCTN.com ISRCTN18365249.
Challenges in rescuing snakes to protect human lives and promote snake conservation in Tamil Nadu, India
Authors: Salim Anika , Williams Jarred , Almeida Jose R. , Chandrasekharuni Gnaneswar , Williams Harry F. , Vaiyapuri Rajendran , Vaiyapuri Mohanraj , Viswanath Rajan , Annamalai Thanigaivel , Patel Ketan , Thirumalaikolundusubramanian Ponniah , Senthilkumaran Subaramanian , Whitaker Romulus , Vaiyapuri Sakthivel
Published: September 2024

Background

Human-snake conflicts are common worldwide, often resulting in snakebites. Snakebite envenoming causes over 125,000 deaths and 400,000 permanent disabilities worldwide every year. India alone accounts for an average of ~58,000 annual snakebite-induced deaths. As human developments rapidly expand into suburban and rural areas, snakes are being displaced and incidences of residents finding snakes within their dwellings are increasing. Most people have an innate fear of snakes, compounded by centuries of negative influence from culture and mythology manifesting in people often attempting to kill snakes. Snake rescuers are volunteers who remove and relocate snakes to safe areas. This is a risky job that poses potentially fatal implications if bitten. These volunteers mostly receive no financial compensation for their time or transportation costs, but they choose to do it for their love of snakes, conservation, and for the altruistic nature of helping others. Snake rescuers often receive no formal training and are unfunded resulting in removing snakes improperly without adequate safety equipment or the required skill set to safely complete the task. Therefore, it is critical to determine their challenges and requirements to promote the safe rescue of snakes while protecting human lives.

Methodology/Principal findings

In this study, we developed an online questionnaire and interviewed 152 snake rescuers in Tamil Nadu, India following written informed consent to determine their challenges and needs for rescuing snakes safely. The results demonstrate that most rescuers are males, and they conduct snake rescues for varying lengths of time. They mostly receive no formal training and are bitten by snakes. They spend their own money on the purchase of snake-handling equipment and on treatments if bitten or injured during a rescue.

Conclusions/Significance

The rescuers highlighted the urgent need for formal training, safety equipment and standard protocols for rescuing snakes in Tamil Nadu. Overall, this study demonstrates that snake rescuing should be appropriately regulated by the authorities, in particular the Wildlife Division of State Forest Departments in India, and formal training along with necessary equipment, medical insurance and appropriate recognition should be provided to them to safely remove snakes from human dwellings and manage the safety of both snakes and humans. They can also act as educators to disseminate information about the preventive and first aid measures for snakebites as well as the ecological importance of snakes.
Incidence and risk of post-COVID-19 thromboembolic disease and the impact of aspirin prescription; nationwide observational cohort at the US Department of Veteran Affairs
Authors: Ware Anna D. , Veigulis Zachary P. , Hoover Peter J. , Blumke Terri L. , Ioannou George N. , Boyko Edward J. , Osborne Thomas F.
Published: September 2024

Introduction

COVID-19 triggers prothrombotic and proinflammatory changes, with thrombotic disease prevalent in up to 30% SARS-CoV-2 infected patients. Early work suggests that aspirin could prevent COVID-19 related thromboembolic disorders in some studies but not others. This study leverages data from the largest integrated healthcare system in the United States to better understand this association. Our objective was to evaluate the incidence and risk of COVID-19 associated acute thromboembolic disorders and the potential impact of aspirin.

Methods

This retrospective, observational study utilized national electronic health record data from the Veterans Health Administration. 334,374 Veterans who tested positive for COVID-19 from March 2, 2020, to June 13, 2022, were included, 81,830 of whom had preexisting aspirin prescription prior to their COVID-19 diagnosis. Patients with and without aspirin prescriptions were matched and the odds of post-COVID acute thromboembolic disorders were assessed.

Results

10.1% of Veterans had a documented thromboembolic disorder within 12 months following their COVID-19 diagnosis. Those with specific comorbidities were at greatest risk. Preexisting aspirin prescription was associated with a significant decrease risk of post-COVID-19 thromboembolic disorders, including pulmonary embolism (OR [95% CI]: 0.69 [0.65, 0.74]) and deep vein thrombosis (OR [95% CI]: 0.76 [0.69, 0.83], but an increased risk of acute arterial diseases, including ischemic stroke (OR [95% CI]: 1.54 [1.46, 1.60]) and acute ischemic heart disease (1.33 [1.26, 1.39]).

Conclusions

Findings demonstrated that preexisting aspirin prescription prior to COVID-19 diagnosis was associated with significantly decreased risk of venous thromboembolism and pulmonary embolism but increased risk of acute arterial disease. The risk of arterial disease may be associated with increased COVID-19 prothrombotic effects superimposed on preexisting chronic cardiovascular disease for which aspirin was already prescribed. Prospective clinical trials may help to further assess the efficacy of aspirin use prior to COVID-19 diagnosis for the prevention of post-COVID-19 thromboembolic disorders.
Graph-based analysis of DNA sequence comparison in closed cotton species: A generalized method to unveil genetic connections
Authors: Khan Riaz Hussain , Salamat Nadeem , Baig A. Q. , Shaikh Zaffar Ahmed , Yousef Amr
Published: September 2024

Graph theory provides a systematic method for modeling and analysing complicated biological data as an effective bioinformatics tool. Based on current trends, the number of DNA sequences in the DNA database is growing quickly. To determine the origin of a species and identify homologous sequences, it is crucial to detect similarities in DNA sequences. Alignment-free techniques are required for accurate measures of sequence similarity, which has been one of the main issues facing computational biologists. The current study provides a mathematical technique for comparing DNA sequences that are constructed in graph theory. The sequences of each DNA were divided into pairs of nucleotides, from which weighted loop digraphs and corresponding weighted vectors were computed. To check the sequence similarity, distance measures like Cosine, Correlation, and Jaccard were employed. To verify the method, DNA segments from the genomes of ten species of cotton were tested. Furthermore, to evaluate the efficacy of the proposed methodology, a K-means clustering method was performed. This study proposes a proof-of-model that utilises a distance matrix approach that promises impressive outcomes with future optimisations to be made to the suggested solution to get the hundred percent accurate result. In the realm of bioinformatics, this paper highlights the use of graph theory as an effective tool for biological data study and sequence comparison. It’s expected that further optimization in the proposed solution can bring remarkable results, as this paper presents a proof-of-concept implementation for a given set of data using the proposed distance matrix technique.
Analyzing cold hardiness (Based on DTA) of one-year-old branches of peaches
Authors: Li Yonghong , Li Jie , Wang Zhaoyuan , Liu Guojian , Wang Yu , Chang Ruifeng , Chen Hu , Tian Qihang , Wang Xiaodi
Published: September 2024

In this study, we conducted a low-temperature exothermic (LTE) investigation on 1-year-old (1a) branches of sixteen peach cultivars through a differential thermal analysis (DTA) procedure. We used a three-point approach to determine the lethal injury temperature (LT-I) of the xylem, the LTE correlation indexes, and the subordinate function value method were applied to compare cold hardiness of sixteen peach varieties. The results showed that the slope of the LT-I for the xylem of sixteen peach cultivars was different, and the LTE indexes were significantly different. Among all the studied varieties, the cold hardiness was strongest in Donghe No.1, followed by Wangjiazhuangmaotao No.2 and Hunchun. Qiuyan and Yanhong are second, and belong to the cold-resistant type; Qiuyi, Okubo, Zhongnongjinhui, and Chunmei, exhibited medium cold hardiness. Zhongtaohongyu, Spring snow, Yufei, and Zhongyou No.8 varieties exhibited low hardiness; while the 21st century, Golden Honey No. 1 and Zhonghuashoutao have the worst cold hardiness and are the weakest cold-hardiness types. In addition, the injury degrees of xylem from LT-I analysis were significantly related to the browning rates (BR) and electrolytic leakage (EI) from traditional low temperature freezing analysis. It is demonstrated that the LTE analysis is a simple, accurate, and practical method for identifying the cold hardiness of 1a branches of peach.
Factors contributing to delays in accessing health facility-based maternal delivery services in Sierra Leone, 2018: A community-based cluster survey
Authors: Saffa Gbessay , Keimbe Charles , Bangalie Andrew , Sheriff Amara Alhaji , Jalloh Babah , Bah Doris , Bangura Fatmata , Tamba Francis , Bangura Henry , Sesay Isha , Kamara Kassim , Gborie Sahr , Teka Hale , Ikoona Eric , Elduma Adel Hussein , Gebru Gebrekrstos Negash
Published: September 2024

Background

With a Maternal Mortality Ratio (MMR) of 516 deaths per 100,000 live-births, Sierra Leone hosts one of the highest maternal mortalities in globally. National data indicates that over 98% of maternal deaths are related to delays in accessing obstetric services. This study sought to examine factors contributing to delays in accessing maternal delivery services as perceived by women in Sierra Leone.

Methods

We conducted a community-based survey among women who delivered from May 1, 2017 to June 30, 2018, in four districts of Sierra Leone. Delay one was defined as perceived delays in deciding to seek facility-based delivery. Delay two was defined as perceived delays reaching the health facility for delivery services. Data on participants’ socio-demographics, delay one, delay two, three and determinants of delays one and two and three were collected using questionnaires. We calculated frequencies and proportions for factors contributing to delays as well as Prevalence Odds Ratios (POR) to identify risk factors for the delays.

Results

A total of 614 mothers were interviewed, median age 28 years (range, 14–52 years). The prevalence of Delay One was 23.3% (143/614), and Delay Two was 26.9% (165/614). Mother with secondary education were associaited with delay one (aPOR = 2.3; 95% CI:1.14, 4.46). These was an association between perceived delay-two and previous pregnancy-related complications (aPOR = 1.6; 95% CI:1.071, 2.538) and poor condition of roads (POR = 2.34; 95%CI, 1.15, 4.77). Additinally, there was an association between delay-three and previous-related complication during last pregnancy (aPOR = 1.9; 95% CI: 1.055, 3.67).

Conclusions

This study revealed a high prevalence of perceived delays one and two for mothers to access obstetric services. Delays were mainly related to transport difficulties, low knowledge of pregnancy-related complications, and costly obstetric services. A practical strategy for birth preparedness and readiness to reduce delays is urgently needed.
The effects of a high-flavonoid corn cultivar on the gastrointestinal tract microbiota in chickens undergoing necrotic enteritis
Authors: Buiatte Vinicius , Proszkowiec-Weglarz Monika , Miska Katarzyna , Dominguez Dorian , Mahmoud Mahmoud , Lesko Tyler , Panek Bryan P. , Chopra Surinder , Jenkins Mark , Lorenzoni Alberto Gino
Published: September 2024

The search for alternative therapies to antimicrobial growth promoters (AGP) in poultry production has gained momentum in the past years because of consumer preference and government restrictions on the use of AGP in animal production. Flavonoids are plant-derived metabolites that have been studied for their health-promoting properties that could potentially be used as an alternative to AGP in poultry. In a previous study, we showed that the inclusion of a flavonoid-rich corn cultivar (PennHFD1) in the diet improved the health of broilers undergoing necrotic enteritis. However, the mechanisms of action by which the PennHFD1-based diet ameliorated necrotic enteritis are unknown. This study describes the microbial diversity and composition of the jejunum and ileum of chickens co-infected with Eimeria maxima and Clostridium perfringens and treated with a high-flavonoid corn-based diet. Luminal content and mucosal samples from the jejunum and ileum were collected for DNA extraction, 16S rRNA amplicon sequencing and data analyses. The infection model and the dietary treatments significantly changed the alfa diversity indices (Mucosal samples: ASVs, P = 0.04; Luminal content samples: ASVs, P = 0.03), and beta diversities (Mucosal samples: P < 0.01, Luminal content: P < 0.01) of the ileal samples but not those of the jejunal samples. The microbial composition revealed that birds fed the high-flavonoid corn diet had a lower relative abundance of C. perfringens compared to birds fed the commercial corn diet. The treatments also changed the relative abundance of other bacteria that are related to gut health, such as Lactobacillus. We concluded that both the infection model and the dietary high-flavonoid corn changed the broilers’ gut microbial diversity and composition. In addition, the decrease in the relative abundance of C. perfringens corroborates with a decrease in mortality and intestinal lesions due to necrotic enteritis. Collecting different segments and sample types provided a broader understanding of the changes in the gut microbiota among treatments.
Optimization of stimulus properties for SSVEP-based BMI system with a heads-up display to control in-vehicle features
Authors: Hamidi Shishavan Hossein , Roy Raheli , Golzari Kia , Singla Abhishek , Zalozhin David , Lohan Danny , Farooq Muhamed , Dede Ercan M. , Kim Insoo
Published: September 2024

Over the years, the driver-vehicle interface has been improved, but interacting with in-vehicle features can still increase distraction and affect road safety. This study aims to introduce brain-machine interface (BMI)- based solution to potentially enhance road safety. To achieve this goal, we evaluated visual stimuli properties (SPs) for a steady state visually evoked potentials (SSVEP)-based BMI system. We used a heads-up display (HUD) as the primary screen to present icons for controlling in-vehicle functions such as music, temperature, settings, and navigation. We investigated the effect of various SPs on SSVEP detection performance including the duty cycle and signal-to-noise ratio of visual stimuli, the size, color, and frequency of the icons, and array configuration and location. The experiments were conducted with 10 volunteers and the signals were analyzed using the canonical correlation analysis (CCA), filter bank CCA (FBCCA), and power spectral density analysis (PSDA). Our experimental results suggest that stimuli with a green color, a duty cycle of 50%, presented at a central location, with a size of 36 cm2 elicit a significantly stronger SSVEP response and enhanced SSVEP detection time. We also observed that lower SNR stimuli significantly affect SSVEP detection performance. There was no statistically significant difference observed in SSVEP response between the use of an LCD monitor and a HUD.
Development of an affordable light emitting diode spectrophotometer paired with a Python program for calibration and linearity testing and the measurement of uranium(VI)
Authors: Vibho Amrutaa , Rogat Courtney , Karavas Emily , Mohammed Rahisa , Ogadi Peace , White Michael , Salois Thomas , Anderson Charles , Prairie Michael W. , Frisbie Seth H. , Gallant Sarah K.
Published: September 2024

Uranium (U) is a radiologically and chemically toxic element that occurs naturally in water, soil, and rock at generally low levels. However, anthropogenic uranium can also leach into groundwater sources due to mining, ore refining, and improper nuclear waste management. Over the last few decades, various methods for measuring uranium have emerged; however, most of these techniques require skilled scientists to run samples on expensive instrumentation for detection or require the pretreatment of samples in complex procedures. In this work, a Schiff base ligand (P1) is used to develop a simple spectrophotometric method for measuring the concentration of uranium (VI) with an accurate and affordable light-emitting diode (LED) spectrophotometer. A test for a higher-order polynomial relationship was used to objectively determine the calibration data’s linearity. This test was done with a Python program on a Raspberry Pi computer that captured the spectrophotometer’s calibration and sample measurement data.
Radioecological characteristics of Siberian roe deer (Capreolus pygargus Pal., 1771) inhabiting locations of nuclear weapon tests
Authors: Panitskiy Andrey , Bazarbaeva Asem , Baigazy Symbat , Alexandrovich Ivan , Larionova Natalya
Published: September 2024

This paper reports the activity concentrations of 137Cs, 90Sr, 239+240Pu, 241Am, and 3Н in the form of tritiated water (НТО) and organically bound tritium (ОBТ) in the tissues and organs of roe deer (Capreolus pygargus Pal., 1771) that inhabit the ‘Degelen’ test location of the Semipalatinsk Test Site. Tissues and organs were sampled from six deer by killing. The activity concentrations of specific radionuclides in the samples were measured using γ-, α-, and β-spectrometry. The radionuclide activity concentrations in the tissues and organs showed considerable variation, for example, 0.6–170 Bq kg-1 for 137Cs and 0.3–2.8×103 Bq kg-1 for 90Sr. The activity concentrations of radionuclides in animal muscular tissue did not exceed permissible values for the meat of wild animals. The tissues and organs in the roe deer were arranged as follows in descending order of their ability to accumulate 137Cs and 90Sr: for 137Cs, muscular tissue–kidneys–lungs–spleen–heart–liver–bone tissue; for 90Sr, bone tissue–liver–lungs–muscular tissue–spleen–heart–kidneys. The activity concentrations of 241Am and 239+240Pu did not exceed the minimum detectable activity. Therefore, no quantitative values could be determined for 241Am, and the 239+240Pu activity concentration could be derived for only one sample: 0.5±0.1 Bq kg-1 (liver). The distribution pattern of these radionuclides in the tissues and organs of the roe deer could not be determined because of insufficient data. The HTO volumetric activity in the tissues and organs of the examined animals ranged from 2.6×10−2 to 77 kBq l-1; activity concentration of OBT, 3.0×10−2 to 16 kBq kg-1; and OBT-to-HTO ratios, 2.0×10−3 to 5.3×102. This ratio can serve as an indicator of how long the examined animals stay in radioactively contaminated ecosystems. Within the ‘Degelen’ site, the activity concentrations of 90Sr and tritium, in the form of HTO and OBT, are expected to be high in the bone tissues, soft tissues, and organs, respectively.
Region of interest determination algorithm of lensless calcium imaging datasets
Authors: Castillo Virgil Christian Garcia , Akbar Latiful , Siwadamrongpong Ronnakorn , Ohta Yasumi , Kawahara Mamiko , Sunaga Yoshinori , Takehara Hironari , Tashiro Hiroyuki , Sasagawa Kiyotaka , Ohta Jun
Published: September 2024

Advances in fluorescence imaging technology have been crucial to the progress of neuroscience. Whether it was specific expression of indicator proteins, detection of neurotransmitters, or miniaturization of fluorescence microscopes, fluorescence imaging has improved upon electrophysiology, the gold standard for monitoring brain activity, and enabled novel methods to sense activity in the brain. Hence, we developed a lightweight and compact implantable CMOS-based lensless Ca2+ imaging device for freely moving transgenic G-CaMP mouse experiments. However, without a lens system, determination of regions of interest (ROI) has proven challenging. Localization of fluorescence activity and separation of signal from noise are difficult. In this study, we report an ROI selection method using a series of adaptive binarizations with a gaussian method and morphological image processing. The parameters for each operation such as the kernel size, sigma and footprint size were optimized. We then validated the utility of the algorithm with simulated data and freely moving nociception experiments using the lensless devices. The device was implanted in the dorsal raphe nucleus to observe pain-related brain activity following a formalin test to stimulate pain. We observed significant increases in fluorescence activity after formalin injection compared to the control group when using the ROI determination algorithm.
Light weights are as effective as heavy weights for muscle activation in the Hammerobics exercise
Authors: Murofushi Koji , Oshikawa Tomoki , Kaneoka Koji , Akuzawa Hiroshi , Mitomo Sho , Hatano Genki , Furuya Hidetaka , Hirohata Kenji , Yagishita Kazuyoshi
Published: September 2024

Purpose

We previously reported that a Hammerobics exercise using weights can enhance trunk and lower extremity muscles, some studies suggested that training effects could also be expected without heavy weights. If the muscle activity induced by Hammerobics swinging with a ’light plastic ball’ equals or surpasses that of the isometric static squat and synchronized squat with a heavy ball, this training approach could alleviate joint strain, fostering an exercise regimen universally accessible across generations, benefit for workouts sports and rehabilitation.

Methods

Fifteen healthy men participated in this study. By using surface electromyography, muscle activities for the abductor hallucis, tibialis anterior, tibialis posterior, peroneus longus, rectus femoris, biceps femoris long head, semitendinosus, gluteus maximus, multifidus, and internal oblique muscles were measured during a light Hammerobics synchronized squat (HSS-light), Hammerobics synchronized squat, and conventional isometric squat, and statistically compared.

Results

The front-to-back tibialis anterior activity of HSS-light was significantly higher than that of conventional isometric squat. The activities of all other muscles, except for the multifidus, were not significantly different between these exercises in the front-to-back and back-to-front phases. Compared to the Hammerobics synchronized squat, the light Hammerobics synchronized squat showed no differences in front-to-back tibialis anterior, rectus femoris, biceps femoris long head, and internal oblique activities and back-to-front abductor hallucis, tibialis anterior, rectus femoris, and internal oblique activities.

Conclusion

The HSS-light could stimulate muscles to the same level as the conventional isometric squat without weight bearing. While the HSS-light was less effective than the Hammerobics synchronized squat, there was no significant difference in internal oblique, rectus femoris, and tibialis anterior activities between these exercises. Hence, the HSS-light is an exercise method that can be viable approach to promoting accessible workouts sports and rehabilitation.
Repurposing auranofin and meclofenamic acid as energy-metabolism inhibitors and anti-cancer drugs
Authors: Rodríguez-Enríquez Sara , Robledo-Cadena Diana Xochiquetzal , Pacheco-Velázquez Silvia Cecilia , Vargas-Navarro Jorge Luis , Padilla-Flores Joaquín Alberto , Kaambre Tuuli , Moreno-Sánchez Rafael
Published: September 2024

Objective

Cytotoxicity of the antirheumatic drug auranofin (Aur) and the non-steroidal anti-inflammatory drug meclofenamic acid (MA) on several cancer cell lines and isolated mitochondria was examined to assess whether these drugs behave as oxidative phosphorylation inhibitors.

Methods

The effect of Aur or MA for 24 h was assayed on metastatic cancer and non-cancer cell proliferation, energy metabolism, mitophagy and metastasis; as well as on oxygen consumption rates of cancer and non-cancer mitochondria.

Results

Aur doses in the low micromolar range were required to decrease proliferation of metastatic HeLa and MDA-MB-231 cells, whereas one or two orders of magnitude higher levels were required to affect proliferation of non-cancer cells. MA doses required to affect cancer cell growth were one order of magnitude higher than those of Aur. At the same doses, Aur impaired oxidative phosphorylation in isolated mitochondria and intact cells through mitophagy induction, as well as glycolysis. Consequently, cell migration and invasiveness were severely affected. The combination of Aur with very low cisplatin concentrations promoted that the effects on cellular functions were potentiated.

Conclusion

Aur surges as a highly promising anticancer drug, suggesting that efforts to establish this drug in the clinical treatment protocols are warranted and worthy to undertake.