bluuebunny/crossref_metadata_2025 huggingface dataset:
Mucin biotinylation - NHS-biotin reagent v2
Authors: Linderberg Victoria
Published: June 2017

This protocol describes how to attach biotin to Bovine Serum Mucin (BSM). The procedure uses a botin-NHS molecule to attach biotin to amines in the mucin proteins.
Protocol for washing and modifying QCM-D sensors v2
Authors: Linderberg Victoria
Published: June 2017

This protocol describes how to wash QCM-D sensors before usage and how to modify their surface using a composition of symmetric (poly)ethylene glycol (PEG) thiolsconsisting of 99% dS-PEG and 1% dS-PEG-biotin.
T7 transcription mix for guide RNAs protocol v1
Authors: Corn Jacob
Published: June 2017

Mix the components below for a total volume of 20 µl.
Gel Electrophoresis v2
Authors: J. Cone Alan
Published: June 2017

Separates molecules based on size. Great for checking DNA after a Restriction Digest.
Intracellular Flow Cytometry Staining (Collection) v1
Authors: not provided BioLegend , Inc. not provided
Published: June 2017

This is a collection of BioLegend protocols for theIntracellular Flow Cytometry Staining, including the Activation andIntracellular Staining of Whole Blood.
C_HW4 (tac) v3
Authors: Youens-Clark Ken
Published: June 2017

Create a script that is the reverse of ‘cat’ in that it will print the lines from a given input file in reverse order: $ cat input.txt foo bar baz quux $ ./tac.pl6 input.txt quux baz bar foo The script should accept a single unnamed “input” argument that should be a file. If provided no arguments or an argument that is not an existing file, the script should produce a “usage” statement: $ ./tac.pl6 Usage: ./tac.pl6  $ ./tac.pl6 this-does-not-exist Usage: ./tac.pl6  If given an input that does not exist, it should print a usage statement. A passing test suite should look like this: $ make test ./test.pl6 1..3 ok 1 - No args gives usage ok 2 - Bad arg gives usage ok 3 - Correct output If you “make jabber,” you should see this: And the mome raths outgrabe. All mimsy were the borogoves, Did gyre and gimble in the wabe; 'Twas brillig, and the slithy toves He chortled in his joy. O frabjous day! Callooh! Callay!' Come to my arms, my beamish boy! "And, has thou slain the Jabberwock? He went galumphing back. He left it dead, and with its head The vorpal blade went snicker-snack! One, two! One, two! And through and through And burbled as it came! Came whiffling through the tulgey wood, The Jabberwock, with eyes of flame, And, as in uffish thought he stood, And stood awhile in thought. So rested he by the Tumtum tree, Long time the manxome foe he sought -- He took his vorpal sword in hand: The frumious Bandersnatch!" Beware the Jubjub bird, and shun The jaws that bite, the claws that catch! "Beware the Jabberwock, my son! And the mome raths outgrabe. All mimsy were the borogoves, Did gyre and gimble in the wabe: 'Twas brillig, and the slithy toves JABBERWOCKY -- Lewis Carroll
Monterey Wharf II Weekly Phytoplankton Monitoring Sample Collection and Processing Notes v5
Authors: woods april
Published: June 2017

A description of how samples are collected and processed as part of MLML SmithLab's weekly HAB monitoring efforts in Monterey, CA. Sampling Location: Monterey Commercial Wharf, Monterey, CA. 36° 36.3' N 121° 53.3' W Oceanographic and meteorological observations for the time of sampling are referenced from the Monterey, CA NOAA station ID: 9413450
Monitoring fluorescence during cyanobacterial physiology/lysis experiments v2
Authors: Giuliani Sarah
Published: June 2017

Monitor fluorescence of cyanobacterial cultures overtime using a 96-well plate format.
Total digestion of marine particles v1
Authors: Pinedo Paulina
Published: June 2017

Totally digest marine particles collected on polyethersulfone (PES, Pall Supor) filters.
MG_HW6: Gene Calls with Prodigal v2
Authors: Thornton James
Published: June 2017

This protocol provides the procedure to generate gene calls on your contigs using Prodigal.
Lysate Preparation from Viruses in Suspension v1
Authors: not provided Bioline
Published: June 2017

The steps for preparing the lysate are different depending on the starting material. Please ensure you follow the correct procedure for your starting material (see the section Disrupting and Homogenizing Starting Materials and the different lysate preparation protocols here). The subsequent steps detailed in Genomic DNA removal and total RNA purification are the same in all cases. Follow this protocol for isolating RNA from free viral particles.
ELISA protocol v2
Authors: not provided ELISAassays
Published: June 2017

Assay protocol for pre-coated ELISA plate to measure protein concentration
CZV8- fungal protoplast agar v1
Authors: Mead Oliver
Published: June 2017

CZV8-proto agar 500 mL 300 mL Czapek-Dox broth 22.7 g 13.62 g agar 5 g 3 g sorbitol 91.1 g 54.7 g centrifuged V8 juice 100 mL 60 mL add H2O to 500 mL 300 mL adjust to pH 6.0 with NaOH autoclave and pour thin plates (approx. 10mL)
Memasukkan foto ke dalam photo frame v2
Authors: Irawan Hendy
Published: June 2017

Cara memasukkan foto mentah (raw) ke dalamphoto frame yang sudah disiapkan dalam format PowerPoint, agar siap dipublikasi di media sosial dan situs web.
MG_HW7: Taxonomic Classification Using Centrifuge v4
Authors: Thornton James
Published: June 2017

This protocol provides a procedure to generate taxonomic data from assembled contigs using centrifuge.
Enumerating algal viruses by flow cytometry v1
Authors: Zimmerman Amy
Published: June 2017

Purpose: To quantify virus-like particles in culture or environmental samples. Summary: Host cells and virus-like particles are enumerated from the same glutaraldehyde-fixed sample. For virus counts, an aliquot is diluted in 0.02-µm-filtered TE buffer prior to staining with SYBR Green I nucleic acid dye to improve staining efficiency and ensure an appropriately low event rate in the instrument. An unstained aliquot of sample is run for host cell counts.
Lysogeny Broth (LB) v1
Authors: Burgess Steven
Published: June 2017

Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The initialism is also commonly, albeit incorrectly, taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth.[1] The formula of the LB medium was published in 1951 in the first paper of Bertani on lysogeny. In this article he described the modified single-burst experiment and the isolation of the phages P1, P2, and P3. He had developed the LB medium to optimize Shigella growth and plaque formation.[1][2] LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.[3][4][5][6][7] These media have been widely used in molecular microbiologyapplications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains ofEscherichia coli.[8] For physiological studies however, the use of LB medium is to be discouraged.[9] There are several common formulations of LB. Although they are different, they generally share a somewhat similar composition of ingredients used to promote growth, including the following: Peptides and casein peptones Vitamins (including B vitamins) Trace elements (e.g. nitrogen, sulfur, magnesium) Minerals Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. In his original 1951 paper, Bertani used 10 grams of NaCl and 1 gram of glucose per 1 L of solution; Luria in his 'L broth' of 1957 copied Bertani's original recipe exactly.[6] Recipes published later have typically left out the glucose. From Wikipeidahttps://en.wikipedia.org/wiki/Lysogeny_broth
Methylene Blue staining v1
Authors: Prakash Manu
Published: June 2017

A simple protocol for staining with Methylene Blue, a very commonly available stain.
GUS Solution v1
Authors: Reeves Greg
Published: June 2017

The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology[1] and microbiology.[2]Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique. (Source:https://en.wikipedia.org/wiki/GUS_reporter_system)
Unix and Bioinformatics v2
Authors: Tully and Ken Youens-Clark Benjamin
Published: June 2017

This protocol details the use of various unix commands commonly used in bioinformatics.
Concentration of Phage Lysate using Vivaflow Tangential/Crossflow Filtration Cassette v1
Authors: Giuliani Sarah
Published: June 2017

Purpose: To concentrate harvested phage lysate up to 20X - 40X. The Vivaflow device uses a peristaltic pump setup to recirculate the input sample over a 100,000 MWCO PES membrane, which allows the media filtrate to be diverted and the phage to remain in the starting sample.
ECOGEO 'Omics Training: 1.0 Unix and Bioinformatics v4
Authors: Tully and Ken Youens-Clark Benjamin
Published: June 2017

This protocol details the use of various unix commands commonly used in bioinformatics. Open this protocol inside the virtual machine (details in "Start Instructions") for easy copy, paste of commands into the command line terminal window.
ECOGEO 'Omics Training: 2.0 Quality Control v2
Authors: Tully Ben
Published: June 2017

Quality impact results is the universal first step for all sequencing methods. This protocol is for sequencing results in FASTQ format. FastQC allows for visualization of quality scores. Sequence trimming allows for removal of residual primer sequences and increases overall quality scores. Trimmomatic does both. CutAdapt cuts adapters from sequences. Sickle creates a sliding window for quality and length trimming of FASTQ files. Open this protocol inside the virtual machine (details in 'Start Instructions') for easy copy, paste of commands into the command line terminal window.
ECOGEO 'Omics Training: 3.2 Amplicon Analysis - QIIME v2
Authors: Dsouza Melissa
Published: June 2017

Commands for hands-on component of QIIME, which should be run from a local installation. Details can be found online at http://qiime.org/install/index.html. The files used in this hands-on component are also available the virtual machine (details in 'Start Instructions'), but you will need to upload them to your Google Drive or Dropbox from within the VM in order to have them available for QIIME, which is installed locally.
Setting up Experimental Cultures for Large-scale One-step Phage Infection of Cyanobacteria v2
Authors: Giuliani Sarah
Published: June 2017

Experiment purpose is to monitor the time-course of a large-scale infection of host cyanobacteria by phage under variable media conditions and obtain samples for proteomic and transcriptomic analysis. As a minimum, prepare 12 sample bottles for cells infected with phage and control phage lysate, under 2 different media treatments, with triplcates of each unique Phage/Treatment combination.
0.25 Potato Dextrose Agar v1
Authors: Stam Remco
Published: June 2017

Growth medium used at the Technical University Munich, Phytopathology, for cultivation of Fusarium spp. Note, we use 1/4 concentration.
scratch, don't use, delete ( the biology is unexpected and something we don't want to invest time in figuring down right now ) v2
Authors: Miller Darach
Published: June 2017

The aim of this protocol is to describe how to do a very reproducible upshift of a nitrogen-limited yeast culture to nitrogen non-limited conditions. In order to do this with enough cells to make inefficient analysis (ie RATEseq) feasible, we start in the chemostat for high-cell counts.
MG_HW2: Downloading SRA data using the SRA toolkit v2
Authors: Thornton James
Published: June 2017

The Sequence Read Archive (SRA) is a database for biological sequence data and ismaintained by the National Center for Biotechnology Information (NCBI). Sequence files can be obtained by using the SRA Toolkit. This protocol provides the stepsnecessary to use the SRA toolkit to get sequence data in fastq format.
C_HW2: Makefile, command line exercises with yeast v10
Authors: Youens-Clark Ken
Published: June 2017

Yeast is a well-characterized genome due to its small size and historical significance in genetics. The websitehttp://yeastgenome.org/ is a dedicated resource for yeast genomics. For this exercise, I want you to create a "Makefile" that will execute this entire pipeline if I type "make."
DNA extraction from fungal mycelium using Extract-n-Amp v2
Authors: U'Ren Jana
Published: June 2017

This is a modified protocol for extracting DNA from fungal myelium using the Sigma-Aldrich Extract-N-Amp Plant Kit published by J.M. U'Ren et al. (2016) Contributions of North American endophytes to the phylogeny, ecology, and taxonomy of Xylariaceae (Sordariomycetes, Ascomycota). Molecular Phylogenetics and Evolution 98: 210-232.
Efficient Large Bottle Cleaning v1
Authors: John Seth
Published: June 2017

This protocol is designed to allow you to clean larger GEOTRACES bottles to the highest standards of cleanliness, but in a time-efficient manner.
MG_HW3: Quality Control and Pre-processing v1
Authors: Thornton James
Published: June 2017

This protocol will introduce a workflow for quality control and pre-processing of metagenomic sequence reads using FastQC for visualization and FastX Toolkit for editing the fastq files.
EpiQuik™ Total Histone Extraction Kit for Tissues (Treated and Untreated v3
Authors: Crowley Natalie
Published: June 2017

The EpiQuik™ Total Histone Extraction Kit is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissuesin a simple 60 minute procedure.The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits or in a variety of downstream applications for histonemethylation, acetylation, phosphorylation,sumoylation, ubiquitination,citrullination, and ADP-ribosylationstudies. Pre-optimized and simple 1 hour protocol. Conveniently includes all essential reagents to carry out a histone extraction. Standardized procedure for reproducible results. Extracts a high yield of total core histones from as little as1mg of tissues. Post-translational modifications are kept intact. Does not affect histone modification status or levels.
Plasmid DNA miniprep protocol for EZ-10 Spin Column Plasmid DNA minipreps kit (BS413), 50 preps v1
Authors: Lucate Joshua
Published: June 2017

This protocol is for the extraction and purification of up to 10ug of plasmid DNA. It can be used for the purification of plasmid DNA ranging from 40 bp to 40kbp.
MG_HW4: Co-assembly using Megahit v1
Authors: Thornton James
Published: June 2017

This protocol describes the procedure for performing a co-assembly of short reads to obtain contigs using the Megahit assembler. This procedure is performed on a node at the UoA HPC due to memory considerations.
MG_HW4: Co-assembly using Megahit v3
Authors: Thornton James
Published: June 2017

This protocol describes the procedure for performing a co-assembly of short reads to obtain contigs using the Megahit assembler. This procedure is performed on a node at the UoA HPC due to memory considerations.
SensiFAST™ Probe No-ROX One-Step Kit v1
Authors: not provided Bioline
Published: June 2017

The SensiFAST™ Probe No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR. The SensiFAST Probe No-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
Growing and harvesting Electro Competent E-coli Cells v1
Authors: Blondel Leo
Published: June 2017

for 500ml (2x250ml)
Zyppy™ Plasmid Miniprep Kit v1
Authors: Research Zymo
Published: June 2017

The Zyppy™ Plasmid Miniprep Kit features a Pellet-Free™ modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, then purify using our Fast-Spin column technology (alternatively, the samples may be processed by the classical centrifugation method). Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization.
SQK-MAP006 Low Input protocol for library preparation for Nanopore sequencing v1
Authors: Istace Benjamin , Anne Friedrich not provided , Léo dAgata not provided , Sébastien Faye not provided , Emilie Payen not provided , Odette Beluche not provided , Claudia Caradec not provided , Sabrina Davidas not provided , Corinne Cruaud not provided , Gianni Liti not provided , Arnaud Lemainque not provided , Stefan Engelen not provided , Patrick Wincker not provided , Joseph Schacherer not provided , Jean-Marc Aury not provided
Published: June 2017

Describes the library preparation for Nanopore sequencing from low input DNA according to the SQK-MAP006 protocol It accompanies the GigaScience publication: Benjamin Istace, et al. (2017) De novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer. GigaScience...
El residuo líquido de las curtiembres estudio de caso: Cuenca alta del río Bogotá
Authors: Corredor Rivera Jorge Luis
Published: June 2017

El tratamiento de pieles de animales para su empleo en la industria marroquinera, del vestido y del calzado, produce residuos líquidos que tienen altas implicaciones nocivas sobre el recurso hídrico. Sobre el tratamiento que debe darse a los residuos líquidos y sólidos provenientes de la industria de las curtiembres se han realizado muchas pruebas y estudios, para optimizar la calidad de la disposición final de los mismos. En este sentido, se comentan algunos de los aspectos físico-químicos de los residuos líquidos relacionados con esta industria y sus efectos sobre el ambiente, así como algunas propuestas para manejarlos con la perspectiva que sean aplicables en el entorno colombiano. Con el fin de dar una idea de tales implicaciones y posibles soluciones, se presenta un somero análisis para la cuenca alta del río Bogotá, en el sector vecino a la cabecera municipal de Villapinzón y el sector de San Benito en la ciudad de Bogotá.
Análisis de desempeño y evaluación de requerimientos AAA en protocolos de seguridad sobre redes inalámbricas IEEE 802.11
Authors: Chaparro Vargas Ramiro Alberto , Mejía Fajardo Marcela
Published: June 2017

El desarrollo de un nuevo esquema de confidencialidad y protección de información, en redes inalámbricas de área local IEEE 802.11 ha traído avances substanciales, incluyendo toda una nueva jerarquía de protocolos para satisfacer cada uno de los servicios de seguridad. Este artículo analiza y evalúa el desempeño y cumplimiento de requerimientos AAA de los protocolos RADIUS y DIAMETER, en entornos inalámbricos.
Diagnóstico del estudio ergonómico en la Universidad Militar Nueva Granada
Authors: Huertas Noguera Diana Carolina
Published: June 2017

El proyecto “Estudio y análisis ergonómico en las oficinas de la Universidad Militar Nueva Granada en los niveles administrativos (asesor, ejecutivo, profesional, técnico y asistencial)”, esta constituido por cuatro fases: diagnóstico, estudio de condiciones ambientales, estudio antropométrico y diseño del plan de capacitación. En este artículo, Nosotros describimos la primera fase, que consiste en evaluar los aspectos ergonómicos de la Universidad Militar. La recolección de información a través de encuestas y formatos de observación y se realizo un análisis estadísticos de un software comercial. Basado en resultados cuatro puntos críticos fueron identificados, iluminación, seguridad industrial, diseño de puestos de trabajo, así como también la percepción de los empleados frente a la ergonomía. Dos factores no críticos fueron identificados, el ruido y la temperatura. Estos serán sujeto de futuras investigaciones.
Discretización exacta de un robot móvil con retardo de transporte
Authors: Niño Suárez Paola , Velasco Villa M. , Aranda Bricaire E.
Published: June 2017

En este trabajo se presenta la obtención del modelo exacto en tiempo discreto de un robot móvil tipo (2,0), bajo la consideración de la existencia de retardos de transporte en la señal de control, producidos por la propagación de las señales entre los sensores y actuadores del robot y el control remoto. El modelo obtenido es evaluado mediante simulación, comparando su desempeño con un modelo discreto aproximado que incluye también retardos de transporte y con el modelo en tiempo continuo del mismo sistema.
La helicicultura en Colombia: reforma normativa y rezago productivo
Authors: Lopez Palechor Leydy Evonne , Ruano Ibarra Elizabeth del Socorro , Brisola Marlon Vinicius
Published: June 2017

A partir de una aproximación histórica-comparativa se analiza la reglamentación colombiana de la helicicultura, en contraste con los datos oficiales sobre el volumen exportable de moluscos y las consideraciones de especialistas en Colombia. El estudio se sitúa en el debate académico sobre la complementariedad entre los ámbitos institucional y asociativo para el fortalecimiento de sectores agroindustriales. La investigación se apoyó en la revisión documental y en la aplicación de un cuestionario estructurado a partir de las variables legislación, estructura productiva y asociatividad. Del análisis se infiere que las modificaciones normativas no impactaron significativamente la producción y la asociatividad. En esa perspectiva se cuestiona la premisa de la eficacia del ámbito normativo en la creación de condiciones favorables para consolidar actividades rurales con potencial exportador.
Circulating LOXL2 Levels Reflect Severity of Intestinal Fibrosis and GALT CD4+ T Lymphocyte Depletion in Treated HIV Infection
Authors: Seang Sophie , Somasunderam Anoma , Nigalye Maitreyee , Somsouk Ma , Schacker Timothy W. , Sanchez Joyce L. , Hunt Peter W. , Utay Netanya S. , Lake Jordan E.
Published: June 2017

Background: Incomplete immune reconstitution may occur despite successful antiretroviral therapy (ART). Gut-associated lymphoid tissue (GALT) fibrosis may contribute via local CD4+ T lymphocyte depletion, intestinal barrier disruption, microbial translocation, and immune activation.Methods: In a cross-sectional analysis, we measured circulating fibrosis biomarker levels on cryopreserved plasma from adult HIV-infected (HIV+) SCOPE study participants on suppressive ART who also had fibrosis quantification on recto-sigmoid biopsies. Relationships among biomarker levels, clinical and demographic variables, GALT lymphoid aggregate (LA) collagen deposition, and LA CD4+ T lymphocyte density were analyzed using simple regression. Biomarker levels were also compared to levels in HIV+ viremic SCOPE participants and a convenience sample of HIV-uninfected (HIV-) samples. Results: HIV+ aviremic participants (n=39) were 92% male and 41% non-white, with median age 48 years, CD4+ T lymphocyte count 277 cells/mm3, and 17 years since HIV diagnosis. Most biomarkers were lower in HIV− (n=36) vs HIV+ aviremic individuals, although CXCL4 levels were higher. HIV+ viremic individuals (N=18) had higher median TGF-ß3, CIC-C1Q, and TIMP-1 (P<0.05) and lower LOXL2 levels (P=0.08) than HIV+ aviremic individuals. Only higher LOXL2 levels correlated with more GALT collagen deposition (R=0.44, P=0.007) and lower LA CD4+ T lymphocyte density (R=−0.32, P=0.05) among aviremic individuals.Conclusions: Circulating LOXL2 levels may be a noninvasive measure of intestinal fibrosis and GALT CD4+T lymphocyte depletion in treated HIV infection. LOXL2 crosslinks elastin and collagen, and elevated LOXL2 levels occur in pathologic states, making LOXL2 inhibition a potential interventional target for intestinal fibrosis and its sequelae.
Le Corbusier 24NC Un fragmento habitado de la Ville Radieuse = Le Corbusier 24NC An inhabited fragment of the Ville Radieuse
Authors: Alonso Pereira José Ramón
Published: June 2017

Considerado siempre como el hábitat corbuseriano por  excelencia, el apartamento-atelier de Le Corbusier en Porte Molitor es una villa individual sobre un edificio colectivo edificado --según su propia expresión-- en condiciones de Ville Radieuse. Radieuse, radiante, es el término empleado para definir la ciudad ideal: una urbe moderna imaginada dentro de un gran parque. Este texto pretende analizar esa casa radiante: el lugar y el espacio donde Le Corbusier vivía y sus modos de  habitarlo a través del tiempo, desde 1934 hasta su muerte, tres décadas más tarde. Porque 24NC supone el paso del proyecto a la vida: de proyectar la Ville Radieuse, a vivir en ella. Abstract Ever considered the corbusian habitat for excellence, the  appartement of Le Corbusier at 24NC Porte Molitor is an individual villa over a rent collective building edified –as he used to say-- on Ville Radieuse conditions. Radieuse, radiant, isthe word used to define the ideal city: a modern urbs imagined within a large park. This paper tries to analyse this maison radieuse: the place and the space where Le Corbusier lived in and his ways of living in along the time, from 1934 to his death in 1965. To study the double aspect of habitat and living. To analyse the place and the space where Le Corbusier lived, at his projects and his metaphors: the maison radieuse, the urbs in ortu, the crystal house, the labour archipielago, with its different islands of paints and books, of papers and memories...  Because 24NC suppose the transit from project to life: from planning Ville Radieuse, to living in.
El Tratado de Vitruvio ilustrado : los dibujos de la basílica = The Vitruvian treaty illustrated. The basilica drawings
Authors: Calduch Cervera Juan
Published: June 2017

Los dibujos del levantamiento de un edificio suponen, ya, un alto grado de abstracción o conceptualización que consiste en traducir en elementos gráficos la información aportada por la imagen sensible. Cuando el paso entre la visión del edificio a  su imagen dibujada está mediatizado por el lenguaje hablado o escrito, el dibujo es más elocuente del pensamiento de quien lo traza que del autor que lo describe o la realidad a la que se remite. Los dibujos de la basílica antigua elaborados a partir del tratado de Vitruvio por Fra Giocondo (1511), Cesariano (1523), Palladio (1556, 1570), Perrault (1673) Galiani (1758) y Ortíz y Sanz (1778), así como sus diferencias, más que la idea del tratadista romano, nos permiten conocer los intereses que movían a sus respectivos autores. Abstract Any drawing representing a building implies by itself a high level of abstraction and conceptualization, because it supposes the translation into graphic elements of all the complex information kept by our senses. This situation becomes even more extreme when this process does not begin from the direct observation of the building, but from a spoken or written language. For this reason, the drawing of a building which has been described in a narration, talks on fact more about its author’s point of view, than about the one who is describing it, or even more than the real building itself. The drawings of the basilicas using Vitruvius’ treatise as a reference, and made by Fra Giocondo (1511),  Cesariano (1523), Palladio (1556, 1570), Perrault (1673),  Galiani (1758) and Ortiz y Sanz (1778), more than talking about roman’s author ideas, allow us to know the thoughts of their respective authors.
A la búsqueda de un método. Dialéctica entre composición, materia y construcción en Curro Inza
Authors: Verdasco Ángel
Published: June 2017

Este artículo se suma a la relectura que se está llevando a cabo en tiempos recientes sobre lo que se denominó organicismo madrileño y Escuela de Madrid de los años 60 y 70 del s.XX. El trabajo se centra en uno de los personajes más significativos: el arquitecto Curro Inza. Se analiza en dos de sus obras de juventud la relación que establece entre composición, materia y construcción, en un intento de aproximarnos con mayor acierto a su enigmática arquitectura orgánica y explicar las razones proyectuales que en ella subyacen. Todo ello, junto con el reconocimiento de una toma de postura matérica cercana al brutalismo, permite desmontar algunas percepciones actuales sobre su trabajo, a la vez que posibilita reconocer estrategias arquitectónicas de candente actualidad.
A ambos lados del Atlántico: dos fábricas de Kevin Roche y John Dinkeloo para Cummins Engine Company
Authors: Sánchez Carrasco Laura
Published: June 2017

Dentro de la trayectoria del estudio KRJDA, ambos edificios se plantearon como nuevas oportunidades para investigar la arquitectura de los espacios industriales. Representarían la búsqueda del style for the job heredado de su maestro, Eero Saarinen, quien ya había colaborado con anterioridad con el cliente de las fábricas, J. Irwin MillerEn , en varios proyectos en  Columbus, Ohio. El objetivo es revisar y comparar los conceptos que definen estos dos proyectos construidos para el mismo cliente pero en países diferentes y con requerimientos funcionales distintos. En la planta de Darlington la cuestión se centrará fundamentalmente en sus soluciones constructivas, mientras que en la de Walesboro se reformuló la tipología y el planteamiento funcional de un edificio industrial. Darlington posee grado II de protección desde 1993 y Walesboro ha sido remodelada respetando el espíritu inicial, mereciendo ambas un lugar destacado en el patrimonio industrial del siglo XX.
Developing and validating an age-independent equation of multi-frequency bioelectrical impedance analysis for estimation of appendicular skeletal muscle mass and establishing sarcopenia cutoff
Authors: Yamada Yosuke , Nishizawa Miyuki , Uchiyama Tomoka , Kasahara Yasuhiro , Shindo Mikio , Miyachi Motohiko , Tanaka Shigeho
Published: June 2017

Background: Appendicular skeletal muscle (or lean) mass (ALM) estimated by dual-energy X-ray absorptiometry (DXA) is considered to be a preferred method for sarcopenia studies. However, DXA is expensive, has limited portability, and requires radiation exposure. Bioelectrical impedance analysis (BIA) is inexpensive, easy to use, and portable; thus BIA might be useful in sarcopenia investigations. However, a large variety of models have been commercially supplied by different companies, and for most consumer products, the equations estimating ALM are not disclosed. It is therefore difficult to use these equations for research purposes. In particular, the BIA equation is often age-dependent, which leads to fundamental difficulty in examining age-related ALM loss. The aims of the current study were: 1) to develop and validate an equation to estimate ALM using multi-frequency BIA (MF-BIA) using theoretical models, and 2) to establish sarcopenia cutoff values using the equation for the Japanese population. Mehotds: We measured height (Ht), weight, and ALM obtained by DXA and a standing-posture 8-electrode MF-BIA (5, 50, 250 kHz) in 756 Japanese individuals aged 18 to 86 years old (222 men and 301 women as developing equation group and 97 men and 136 women as a cross validation group). The traditional impedance index (Ht2/Z50) and impedance ratio of high and low frequency (Z250/Z5) of hand to foot values were calculated. Multiple regression analyses were conducted with ALM as dependent variable in men and women separately. Results: We created the following equations: ALM = [0.6947 × (Ht2/Z50)] + [-55.24 × (Z250/Z5)] + [-10940 × (1/Z50)] + 51.33 for men, and ALM = [0.6144 × (Ht2/Z50)] + [-36.61 × (Z250/Z5)] + [-9332 × (1/Z50)] + 37.91 for women. Additionally, we conducted measurements in 1624 men and 1368 women aged 18 to 40 years to establish sarcopenia cutoff values in the Japanese population. The mean values minus 2 standard deviations of the skeletal muscle mass index (ALM/Ht2) in these participants were 6.8 and 5.7 kg/m2 in men and women, respectively. Conclusion: the current study established and validated a theoretical and age-independent equation using MF-BIA to estimate ALM and provided reasonable sarcopenia cutoff values.
Emergency Department Visits Following Elective Total Hip and Knee Replacement Surgery: Identifying Gaps in Continuity of Care
Authors: Finnegan Micaela A. , Shaffer Robyn , Remington Austin , Kwong Jereen , Curtin Catherine , Hernandez-Boussard Tina
Published: June 2017

Background:

Major joint replacement surgical procedures are common, elective procedures with a care episode that includes both inpatient readmissions and postoperative emergency department (ED) visits. Inpatient readmissions are well studied; however, to our knowledge, little is known about ED visits following these procedures. We sought to characterize 30-day ED visits following a major joint replacement surgical procedure.

Methods:

We used administrative records from California, Florida, and New York, from 2010 through 2012, to identify adults undergoing total knee and hip arthroplasty. Factors associated with increased risk of an ED visit were estimated using hierarchical regression models controlling for patient variables with a fixed hospital effect. The main outcome was an ED visit within 30 days of discharge.

Results:

Among the 152,783 patients who underwent major joint replacement, 5,229 (3.42%) returned to the inpatient setting and 8,883 (5.81%) presented to the ED for care within 30 days. Among ED visits, 17.94% had a primary diagnosis of pain and 25.75% had both a primary and/or a secondary diagnosis of pain. Patients presenting to the ED for subsequent care had more comorbidities and were more frequently non-white with public insurance relative to those not returning to the ED (p < 0.001). There was a significantly increased risk (p < 0.05) of isolated ED visits with regard to type of insurance when patients with Medicaid (odds ratio [OR], 2.28 [95% confidence interval (CI), 2.04 to 2.55]) and those with Medicare (OR, 1.38 [95% CI, 1.29 to 1.47]) were compared with patients with private insurance and with regard to race when black patients (OR, 1.38 [95% CI, 1.25 to 1.53]) and Hispanic patients (OR, 1.12 [95% CI, 1.03 to 1.22]) were compared with white patients. These increases in risk were stronger for isolated ED visits for patients with a pain diagnosis.

Conclusions:

ED visits following an elective major joint replacement surgical procedure were numerous and most commonly for pain-related diagnoses. Medicaid patients had almost double the risk of an ED or pain-related ED visit following a surgical procedure. The future of U.S. health-care insurance coverage expansions are uncertain; however, there are ongoing attempts to improve quality across the continuum of care. It is therefore essential to ensure that all patients, particularly vulnerable populations, receive appropriate postoperative care, including pain management.

Level of Evidence:

Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Acetabular Retroversion, but Not Increased Acetabular Depth or Coverage, in Slipped Capital Femoral Epiphysis
Authors: Hesper Tobias , Bixby Sarah D. , Kim Young-Jo , Yen Yi-Meng , Bowen Garrett , Miller Patricia , Millis Michael B. , Novais Eduardo N.
Published: June 2017

Background:

Femoral head overcoverage by a deep and retroverted acetabulum has been postulated as a mechanical factor in slipped capital femoral epiphysis (SCFE). We assessed acetabular depth, coverage, and version in the hips of patients with unilateral SCFE; in the contralateral, uninvolved hips; and in healthy control hips.

Methods:

Thirty-six patients affected by unilateral SCFE were matched to 36 controls on the basis of sex and age. The acetabular depth-width ratio (ADR), the lateral center-edge angle (LCEA), the anterior and posterior acetabular sector angle (ASA), and version (10 mm distal to the highest point of the acetabular dome and at the level of the femoral head center) were assessed on computed tomography (CT). A repeated-measures analysis of variance was used to assess differences among the SCFE, contralateral, and matched-control hips. Pairwise comparisons were conducted using Bonferroni correction for multiple comparisons.

Results:

The mean coronal ADR was significantly lower in the hips affected by SCFE (311.6) compared with the contralateral hips (336.1) (p = 0.001) but did not differ from that of controls (331.9) (p = 0.08). The mean LCEA was significantly lower in the SCFE hips (29.8°) compared with the contralateral hips (33.7°) (p < 0.001) but did not differ from that of controls (32.2°) (p = 0.25). The mean anterior ASA did not differ between the SCFE hips (65.0°) and the contralateral hips (66.0°) (p = 0.68) or the control hips (64.5°) (p = 1.00). The mean posterior ASA in the SCFE hips (92.5°) was significantly lower than that in the contralateral hips (96.5°) (p = 0.002), but no difference was observed between the SCFE hips and controls (96.0°) (p = 0.83). The acetabulum was retroverted cranially in the SCFE hips compared with the contralateral hips (2.7° versus 6.6°; p = 0.01) and compared with controls (2.7° versus 9.6°; p = 0.005). A lower mean value for acetabular version at the level of the femoral head center was also observed in the SCFE hips compared with the contralateral hips (13.9° versus 15.5°; p = 0.04) and compared with controls (13.9° versus 16.0°; p = 0.045). No significant difference (p > 0.05) in acetabular measurements was observed between the contralateral and control hips.

Conclusions:

In SCFE, the acetabulum has reduced version but is not deeper, nor is there acetabular overcoverage. Additional longitudinal studies will clarify whether acetabular retroversion is a primary abnormality influencing the mechanics of SCFE development or an adaptive response to the slip. Our data suggest that the contralateral, uninvolved hip in patients with unilateral SCFE has normal acetabular morphology.

Level of Evidence:

Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Ochrobactrum anthropi used to control ammonium for nitrate removal by starch-stabilized nanoscale zero valent iron
Authors: Zhou Jun , Sun Qianyu , Chen Dan , Wang Hongyu , Yang Kai
Published: June 2017

In this study, the hydrogenotrophic denitrifying bacterium Ochrobactrum anthropi was added in to the process of nitrate removal by starch-stabilized nanoscale zero valent iron (nZVI) to minimize undesirable ammonium. The ammonium control performance and cooperative mechanism of this combined process were investigated, and batch experiments were conducted to discuss the effects of starch-stabilized nZVI dose, biomass, and pH on nitrate reduction and ammonium control of this system. The combined system achieved satisfactory performance because the anaerobic iron corrosion process generates H2, which is used as an electron donor for the autohydrogenotrophic bacterium Ochrobactrum anthropi to achieve the autohydrogenotrophic denitrification process converting nitrate to N2. When starch-stabilized nZVI dose was increased from 0.5 to 2.0 g/L, nitrate reduction rate gradually increased, and ammonium yield also increased from 9.40 to 60.51 mg/L. Nitrate removal rate gradually decreased and ammonium yield decreased from 14.93 to 2.61 mg/L with initial OD600 increasing from 0.015 to 0.080. The abiotic Fe0 reduction process played a key role in nitrate removal in an acidic environment and generated large amounts of ammonium. Meanwhile, the nitrate removal rate decreased and ammonium yield also reduced in an alkaline environment.
Social Media for Social Justice in Adult Education: A Critical Theoretical Framework
Authors: Careless Erin
Published: June 2017

Social media as a communicative forum is relatively new, having been around for only ten years. However, this form of digital engagement has revolutionized the way many people interact, network, form relationships, learn, generate and share knowledge. As a noncentralized tool for communication, social media may provide space for critical discourse around issues of social justice, as discussion can be global in scope and is controlled by users themselves. This paper outlines a critical theoretical framework through which to explore the use of social media in adult education to foster such critical and social justice-themed discourse. Drawing upon five critical theorists and their work, this framework sets the stage for a future research project – one that is significant for this increasingly digital world in which we live.
GLOBAL GOVERNANCE IN ALL ITS DISCRETE FORMS: THE GAME, FIFA, AND THE THIRD WORLD
Authors: Ugochukwu Basil
Published: June 2017

This paper uses the governance praxis of the Federation of International Football Associations [FIFA] to illustrate the impact of several intensive, discrete, and rarely-studied global governance actors whose internal processes and procedures mirror the core concerns of Third World Approaches to International Law [TWAIL] scholars regarding the legitimation of a hegemonic category and the marginalization of Third World and subaltern interests. It is argued that FIFA has become an important international organization and global governance actor whose transnational rule-making characteristics should be studied in light of the incipient migration from “international law” to “global governance”.

     It will be shown that not only are FIFA’s rules impinging on sovereign imagination but that the tendencies of inequality, unfairness and domination afflicting the practices of traditional or state-centric international organizations are as prevalent in the procedures of such less-studied global governance actors regardless that their rule-making activities exert significant impact on governments, especially those in Africa and other parts of the Third World. More significantly, the essay looks at possible domestic political and socio-legal implications of discrete globalization of the kind exemplified by FIFA on Africa and the Third World and how important it is to integrate this concern into TWAIL scholarship going forward.
Tactile Paths: on and through Notation for Improvisers
Authors: Christopher Williams
Published: June 2017

Tactile Paths is a native digital, media-rich PhD dissertation. It aims to articulate and expand the nexus of notation and improvisation in contemporary and experimental music. The project interweaves direct artistic experience with insights from improvisation studies, the social sciences, philosophy, and various scholarship in the arts to reveal methodological connections among diverse artists such as Richard Barrett, Cornelius Cardew, Malcolm Goldstein, Lawrence Halprin, Bob Ostertag, Ben Patterson, and the author. By focusing on how notation is used, rather than on what it represents in an abstract sense, the author shows how written scores emerge from and feed back into ongoing improvisational processes. Thus, it is argued, they are not fixed texts whose primary purpose is to prescribe and preserve, but rather tactile paths in the improviser’s ever-crescent musical and social environment. This practice-based approach aims to lay the conceptual groundwork for theorizing and broadening the creative relevance of work whose importance to practitioners belies its marginal presence in academia and institutions.
Research Appendices
Authors: Tero Nauha
Published: June 2017

Appendix for the doctoral research
BSIDES
Authors: Diego Gil
Published: June 2017

BSIDES is a choreographic project that researches the concept of the refrain as is found in Gilles Deleuze and Felix Guattari philosophy book "A thousand plateaus".

The refrain is a rhytmic movement out and in of chaos and between different signs and codes. In its passage from one territory to the other the refrain transforms (transduces) the codes or signs that encounters.

BSIDES gives form to the concept of the refrain through the actions of reciting and singing poems and songs; crossing different aesthetic territories.

From a single performer with more than 40 years of experience on stage to a group of performers in a process of formation; from an abstract to a pop environment; and from the point of view of self invention to the one of re appropriation.

Lastly the refrain points to a common gesture of any artistic practice. It is a song that one sings in order to find some stability, when walking to the unstable dimensions of the unknown.
Ghetto Child
Authors: Patrick Bobilin
Published: June 2017

An essay responding to the respective site-specificity of both online and printed art criticism. The essay also discusses the importance of short-range broadcast medium for the purpose of cultivating regional art critical "dialects".
Novel
Authors: Zoe Panagiota (aka Betty) Nigianni
Published: June 2017

Single channel digital video, with photography and text, 5' 41'', 2007

'Novel' is a half-photography, half-fiction digital video, which was inspired by my temporary dwelling in different cities during the period of a year. For my contribution to this catalogue, I briefly discuss the artistic process and thinking behind the project. In parallel, I reflect upon the possible theoretical links between visuality, narratives, architecture, and urbanism. I suggest that personal associations are definitive and significant in the ways we tend to occupy and subjectively interpret contemporary urban spaces.

After a lengthy process of collecting photographs and digital film footage, as well as selecting written material, some of it in the form of quotes, from my journal, I completed the video in 2007. My method was:
- I made preparatory sketches that I included in the video (film still 1).
- I used a montage technique I saw in one of Peter Greenaway's films: the separate 'window' on the top or bottom sides of the video (film stills 2, 4, 5) that denotes parallel action.
- I overlaid photographs (film still 3), a technique that allowed me to create the animating effect of the moving image, without using film footage.

In painting, the artist can make layers out of collaged material that might be unconnected or purposefully juxtaposed to the painting's subject. Using the above method, I asked the viewer to watch the video more like a moving series of paintings, rather than as a film.

The video, like my other video art work, was exhibited and archived under my artist's pseudonym, Betty Nigianni.

Written in 2012, the essay is a separate piece to accompany this exposition.

Note: Please disregard comment I posted about a notification that doesn't appear anymore.
sin ∞ fin - The Movie | A performance-based art film project by VestAndPage (Andrea Pagnes & Verena Stenke)
Authors: Andrea Pagnes
Published: June 2017

Inspired by Peter Sloterdijk’s investigation dissecting Micro- and Macro- spherology in his trilogy Spheres, and by Italo Calvino’s novel Invisible Cities, the moving image project sin ∞ fin – The Movie by VestAndPage is based on various stages of research to conjugate performance art with filmmaking. Its final result consists of an art film trilogy produced along the course of three years in the following artist-in-residence programs: CONFL!CTA Contemporary Art and Science Research (Punta Arenas, Chile, 2010); Sarai CSDS Centre for the Studies of Developing Societies (New Delhi, India, 2011); Cultural Program of the DNA Dirección Nacional del Antártico (Antarctica / Buenos Aires, Argentina, 2012), and based on performances conceived site-specific.