bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic variation for terminal heat stress tolerance in winter wheat
Authors: Fu Jianming , Bowden Robert L. , Jagadish S. V. Krishna , Prasad P. V. Vara
Published: February 2023

In many regions worldwide wheat (Triticum aestivum L.) plants experience terminal high temperature stress during the grain filling stage, which is a leading cause for single seed weight decrease and consequently for grain yield reduction. An approach to mitigate high temperature damage is to develop tolerant cultivars using the conventional breeding approach which involves identifying tolerant lines and then incorporating the tolerant traits in commercial varieties. In this study, we evaluated the terminal heat stress tolerance of 304 diverse elite winter wheat lines from wheat breeding programs in the US, Australia, and Serbia in controlled environmental conditions. Chlorophyll content and yield traits were measured and calculated as the percentage of non-stress control. The results showed that there was significant genetic variation for chlorophyll retention and seed weight under heat stress conditions. The positive correlation between the percent of chlorophyll content and the percent of single seed weight was significant. Two possible mechanisms of heat tolerance during grain filling were proposed. One represented by wheat line OK05723W might be mainly through the current photosynthesis since the high percentage of single seed weight was accompanied with high percentages of chlorophyll content and high shoot dry weight, and the other represented by wheat Line TX04M410164 might be mainly through the relocation of reserves since the high percentage of single seed weight was accompanied with low percentages of chlorophyll content and low shoot dry weight under heat stress. The tolerant genotypes identified in this study should be useful for breeding programs after further validation.
Unravelling the relationship between perceived values-congruence with organizational change readiness: A moderated mediation model
Authors: Deng Jinzhao , Cheng Zhihui , Qi Siqi , Deng Rich
Published: February 2023

Recent studies have demonstrated that organizations often fail to execute organizational changes effectively due to a lack of their employees’ organizational change readiness (OCR). However, the absence of employees’ OCR is rooted in whether their values align with their organizations. The research aims to clarify when and why employees’ perceived values-congruence with their organizations, supervisors, and workgroups (PVC-O, PVC-S, and PVC-G) helps stimulate their organizational change readiness (OCR). Specifically, it Integrates the self-categorization theory and social information processing theory and proposes a moderated mediation model to investigate the roles of perceived insider status (PIS) and the quality of change communication (QCC). This study gathered a valid sample of 252 employees from six Chinese companies at three different time points, and performed the structural equation modeling and multiple regression to test the proposed research model. The results demonstrate that PVC-O, PVC-S, and PVC-G are all positively related to employees’ PIS, which further promotes their readiness for organizational change. Additionally, QCC strengthens not only the positive effect of employees’ PVC-O and PVC-G (except for PVC-S) on PIS but also the indirect effects of PIS. This study offers valuable implications for practitioners implementing their organizational change practices in China. Moreover, this study can contribute to the organizational change literature by uncovering the underlying mechanism between perceived values-congruence and employees’ OCR in the light of the person-environment interaction.
Vestibular cognition assessment system: Tablet-based computerized visuospatial abilities test battery
Authors: Huang Yan , Zhang Xuehao , Tang Jia , Xia Yuqi , Yang Xiaotong , Zhang Yanmei , Wei Chaogang , Ruan Ruiqi , Ying Hang , Liu Yuhe
Published: February 2023

Introduction

The vestibular system is anatomically connected to extensive regions of the cerebral cortex, hippocampus, and amygdala. However, studies focusing on the impact of vestibular impairment on visuospatial cognition ability are limited. This study aimed to develop a mobile tablet-based vestibular cognitive assessment system (VCAS), enhance the dynamic and three-dimensional (3D) nature of the test conditions, and comprehensively evaluate the visuospatial cognitive ability of patients with vestibular dysfunction.

Materials and methods

First, the VCAS assessment dimensions (spatial memory, spatial navigation, and mental rotation) and test content (weeding, maze, card rotation, and 3D driving tests) were determined based on expert interviews. Second, VCAS was developed based on Unity3D, using the C# language and ILruntime hot update framework development technology, combined with the A* algorithm, prime tree algorithm, and dynamic route rendering. Further, the online test was built using relevant game business logic. Finally, healthy controls (HC) and 78 patients with vertigo (VP) were recruited for the VCAS test. The validity of VCAS was verified using the test results of random controls.

Results

In the weeding test, the HC group had a significantly longer span and faster velocity backward than did the VP group. In the 12 × 12 maze, statistically significant differences in step and time were observed between the two groups, with VP taking longer time and more steps. In the mental rotation task, no significant difference was observed between the two groups. Similarly, no significant difference was found in the performance of the two groups on maps 2, 3, and 4 in the 3D driving task.

Discussion

Thus, impaired visuospatial cognition in patients with vestibular dysfunction is primarily related to spatial memory and navigation. VCAS is a clinically applicable visuospatial cognitive ability test for VP.
Physical activity as a causal variable for adolescent resilience levels: A cross-lagged analysis
Authors: Guo Lingling , Liang Lulu
Published: February 2023

This study extends research on the relationship between physical activity and adolescent resilience by using cross-lagged analysis. Therefore, it used the Adolescent Resilience Rating Scale and the Physical Activity Scale to conduct a one-year longitudinal follow-up survey on 818 adolescents (50.6% boys), aged 12–17. They completed the questionnaires, providing data on physical activity and adolescent resilience. The results indicated there were significant gender differences in physical activity, but there was no significant gender difference in adolescent resilience; there was a significant positive correlation between physical activity and adolescent resilience; physical activity had a significant predictive effect on adolescent resilience. This study supports the assertion that physical activity has an important impact on adolescent resilience. It further analyzes the possible reasons for this result and contemplates the theoretical and practical significance of the findings, which provides evidence for the study of adolescents’ physical and mental health and enriches the theory of resilience.
The influence of job search stress on college students’ addictive social media use: Seeking of social support and perceived social support as serial mediators and sense of coherence as a moderator
Authors: Mao Eric (Zeqing) , Zhao Lishou
Published: February 2023

Resulting from an enlarged number of graduating college students and shrinkage of work opportunities, stress in relation to job search and employment is becoming an increasingly noticeable issue in China. Previous psychiatry research has suggested that social support can be conducive to reducing stress from multiple sources, while the effectiveness hinges on whether it is actually recognized and perceived by the recipients. The prevalence of social media has greatly facilitated the communication and exchange of social support information. However, they can also lead to overuse and addiction problems. This study aims to investigate how job search stress affects graduating college students’ social media addiction severity using a serial mediation model and test the potential moderation effect of sense of coherence. Based on a sample of graduating college students (n= 144), our findings point out a significant pathway for the impacts of stress sequentially through seeking of social support and perceived social support. Furthermore, job search stress seems to have pronounced effects on the psychological need for social support only at low- and mid-levels of sense of coherence.
Comparison of personality differences of Polish mountaineers
Authors: Piepiora Paweł , Bagińska Justyna , Witkowski Kazimierz , Nakonieczna Justyna , Piepiora Zbigniew
Published: February 2023

A noticeably increased interest in mountain climbing, both as the form of extreme sport and a form of tourism, has been observed in Poland recently. The assumption of this study is that practicing different varieties of mountaineering influences the personality of Polish climbers in a different manner. The aim of the research was to compare the personality differences of Polish mountaineers. To this aim, the population of Polish high-performance mountaineers was studied (N = 81; including 39 women and 42 men). Due to the type of mountaineering practiced, the respondents were divided into Alpine climbers (n = 48) and Himalayan climbers (n = 33). The average age of the surveyed climbers is 33.85 years. The Big Five model was used including the NEO-FFI Personality Questionnaire and the analyzes were performed using the IBM SPSS Statistics statistical method package, version 27.0. Statistically significant differences were noted only for agreeableness F(1.77) = 5.05, p = 0.027. The Alpine climbers showed a higher level of agreeableness than the Himalayan climbers. After taking into account the Sidak amendment, significant differences in the level of agreeableness were found only among women. Comparisons between other personality traits were not statistically significant. There is a significant difference between the personalities of Polish Alpine climbers and Polish Himalayan climbers in the dimension of agreeableness only among women: female Alpine mountaineers are more agreeable than Himalayan mountaineers. It was presumed that in terms of ethics in the high mountains, the social competences of Alpine mountaineers are much more developed than that of Himalayan mountaineers.
Does the current state of biomarker discovery in autism reflect the limits of reductionism in precision medicine? Suggestions for an integrative approach that considers dynamic mechanisms between brain, body, and the social environment
Authors: Loth Eva
Published: February 2023

Over the past decade, precision medicine has become one of the most influential approaches in biomedical research to improve early detection, diagnosis, and prognosis of clinical conditions and develop mechanism-based therapies tailored to individual characteristics using biomarkers. This perspective article first reviews the origins and concept of precision medicine approaches to autism and summarises recent findings from the first “generation” of biomarker studies. Multi-disciplinary research initiatives created substantially larger, comprehensively characterised cohorts, shifted the focus from group-comparisons to individual variability and subgroups, increased methodological rigour and advanced analytic innovations. However, although several candidate markers with probabilistic value have been identified, separate efforts to divide autism by molecular, brain structural/functional or cognitive markers have not identified a validated diagnostic subgroup. Conversely, studies of specific monogenic subgroups revealed substantial variability in biology and behaviour. The second part discusses both conceptual and methodological factors in these findings. It is argued that the predominant reductionist approach, which seeks to parse complex issues into simpler, more tractable units, let us to neglect the interactions between brain and body, and divorce individuals from their social environment. The third part draws on insights from systems biology, developmental psychology and neurodiversity approaches to outline an integrative approach that considers the dynamic interaction between biological (brain, body) and social mechanisms (stress, stigma) to understanding the origins of autistic features in particular conditions and contexts. This requires 1) closer collaboration with autistic people to increase face validity of concepts and methodologies; (2) development of measures/technologies that enable repeat assessment of social and biological factors in different (naturalistic) conditions and contexts, (3) new analytic methods to study (simulate) these interactions (including emergent properties), and (4) cross-condition designs to understand which mechanisms are transdiagnostic or specific for particular autistic sub-populations. Tailored support may entail both creating more favourable conditions in the social environment and interventions for some autistic people to increase well-being.
Development and validation of a risk prediction model for early diabetic peripheral neuropathy based on a systematic review and meta-analysis
Authors: Liu Xixi , Chen Dong , Fu Hongmin , Liu Xinbang , Zhang Qiumei , Zhang Jingyun , Ding Min , Wen Juanjuan , Chang Bai
Published: February 2023

Background

Early identification and intervention of diabetic peripheral neuropathy is beneficial to improve clinical outcome.

Objective

To establish a risk prediction model for diabetic peripheral neuropathy (DPN) in patients with type 2 diabetes mellitus (T2DM).

Methods

The derivation cohort was from a meta-analysis. Risk factors and the corresponding risk ratio (RR) were extracted. Only risk factors with statistical significance were included in the model and were scored by their weightings. An external cohort were used to validate this model. The outcome was the occurrence of DPN.

Results

A total of 95,604 patients with T2DM from 18 cohorts were included. Age, smoking, body mass index, duration of diabetes, hemoglobin A1c, low HDL-c, high triglyceride, hypertension, diabetic retinopathy, diabetic kidney disease, and cardiovascular disease were enrolled in the final model. The highest score was 52.0. The median follow-up of validation cohort was 4.29 years. The optimal cut-off point was 17.0, with a sensitivity of 0.846 and a specificity of 0.668, respectively. According to the total scores, patients from the validation cohort were divided into low-, moderate-, high- and very high-risk groups. The risk of developing DPN was significantly increased in moderate- (RR 3.3, 95% CI 1.5–7.2, P = 0.020), high- (RR 15.5, 95% CI 7.6–31.6, P < 0.001), and very high-risk groups (RR 45.0, 95% CI 20.5–98.8, P < 0.001) compared with the low-risk group.

Conclusion

A risk prediction model for DPN including 11 common clinical indicators were established. It is a simple and reliable tool for early prevention and intervention of DPN in patients with T2DM.
Contribution and effectiveness of ski and pole forces in selected roller skiing techniques on treadmill at moderate inclines
Authors: Zhao Shuang , Lindinger Stefan , Ohtonen Olli , Linnamo Vesa
Published: February 2023

Background

Most of the studies about the effects of incline on cross-country skiing are related to the metabolic efficiency. The effective skiing biomechanics has also been indicated to be among the key factors that may promote good performance. The aims of this study were to provide biomechanical characteristics and investigate the relative contribution and effectiveness of ski and pole forces in overcoming the total external resistance with double poling (DP) and Gear 3 (G3) techniques at varying moderate uphill inclines.

Methods

10 male cross-country skiers participated in this study. Custom-made force measurement bindings, pole force sensors, and an 8-camera Vicon system were used to collect force data and ski and pole kinematics at 3°, 4° and 5° with 10 km/h skiing speed.

Results

The cycle length (CL) decreased by 10% and 7% with DP and G3 technique from 3° to 5° (p < 0.001,p < 0.001). The cycle rate (CR) increased by 13% and 9% from 3° to 5° with DP and G3 technique respectively. From 3° to 5°, the peak pole force increased by 25% (p < 0.001) and 32% (p < 0.001) with DP and G3 technique. With DP technique, the average cycle propulsive force (ACPF) increased by 46% (p < 0.001) from 3° to 5°and with G3 technique, the enhancement for ACPF was 50% (p < 0.001). In G3 technique, around 85% was contributed by poles in each incline.

Conclusion

The higher power output in overcoming the total resistance was required to ski at a greater incline. With DP technique, the upper body demands, and technical effectiveness were increasing with incline. With G3 technique, the role of external pole work for propulsion is crucial over different terrains while role of legs may stay more in supporting the body against gravity and repositioning body segments.
Comparison of sagittal spinal alignment on standing plain x-rays and supine MRI in degenerative lumbar disease
Authors: Fan Xiaolong , Xue Deting , Pan Zhijun , Wang Yulu
Published: February 2023

Background

The purpose of the present study is to examine the possible correlation between standing plain x-rays and supine magnetic resonance imaging (MRI) for evaluating spinal sagittal alignment in degenerative lumbar disease (DLD).

Methods

The characteristics and images of 64 patients with DLD were reviewed retrospectively. The thoracolumbar junction kyphosis (TJK), lumbar lordosis (LL) and sacral slope (SS) were measured on lateral plain x-rays and by MRI. Inter- and intra-observer reliability was tested using intra-class correlation coefficients.

Results

The results suggested that TJK measurements obtained from MRI tended to underestimate the radiographic measures by 2°, whereas SS measurements obtained from MRI tended to overestimate the radiographic measures by 2°. The LL measurements obtained from MRI were approximately equal to the radiographic measures, and the x-ray and MRI measurements were linearly related.

Conclusions

In conclusion, supine MRI can be directly translated into sagittal alignment angle measurements obtained from standing x-rays with an acceptable degree of accuracy. This can avoid the impaired view caused by the overlapping ilium, while reducing the patient's exposure to radiation.
Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms
Authors: Nizovtseva Irina , Palmin Vladimir , Simkin Ivan , Starodumov Ilya , Mikushin Pavel , Nozik Alexander , Hamitov Timur , Ivanov Sergey , Vikharev Sergey , Zinovev Alexei , Svitich Vladislav , Mogilev Matvey , Nikishina Margarita , Kraev Simon , Yurchenko Stanislav , Mityashin Timofey , Chernushkin Dmitrii , Kalyuzhnaya Anna , Blyakhman Felix
Published: February 2023

Development of energy-efficient and high-performance bioreactors requires progress in methods for assessing the key parameters of the biosynthesis process. With a wide variety of approaches and methods for determining the phase contact area in gas–liquid flows, the question of obtaining its accurate quantitative estimation remains open. Particularly challenging are the issues of getting information about the mass transfer coefficients instantly, as well as the development of predictive capabilities for the implementation of effective flow control in continuous fermentation both on the laboratory and industrial scales. Motivated by the opportunity to explore the possibility of applying classical and non-classical computer vision methods to the results of high-precision video records of bubble flows obtained during the experiment in the bioreactor vessel, we obtained a number of results presented in the paper. Characteristics of the bioreactor’s bubble flow were estimated first by classical computer vision (CCV) methods including an elliptic regression approach for single bubble boundaries selection and clustering, image transformation through a set of filters and developing an algorithm for separation of the overlapping bubbles. The application of the developed method for the entire video filming makes it possible to obtain parameter distributions and set dropout thresholds in order to obtain better estimates due to averaging. The developed CCV methodology was also tested and verified on a collected and labeled manual dataset. An onwards deep neural network (NN) approach was also applied, for instance the segmentation task, and has demonstrated certain advantages in terms of high segmentation resolution, while the classical one tends to be more speedy. Thus, in the current manuscript both advantages and disadvantages of the classical computer vision method (CCV) and neural network approach (NN) are discussed based on evaluation of bubbles’ number and their area defined. An approach to mass transfer coefficient estimation methodology in virtue of obtained results is also represented.
Floristic Inventory of Ethnobotanically Important Halophytes of North-Western Mediterranean Coastal Brackish Areas, Tuscany, Italy
Authors: Lombardi Tiziana , Ventura Irene , Bertacchi Andrea
Published: February 2023

Plants have always been used by people for multiple purposes, but over the centuries knowledge of useful plants has largely been lost. Through ethnobotanical studies it is possible to retrieve information on the uses of plants and renew the ancient attention to plants which could be useful to apply in modern applications. In this context, the ethnobotanical use of halophytes has not been explored in depth. The present study focused on the flora of two brackish areas of the north-western Mediterranean sited in Tuscany, Italy. This research aimed to identify the halophyte species of ethnobotanical interest and create a relative map database of the study areas. The floristic list of the two areas, including 78 halophyte and non-halophyte species, was created following periodic field sampling, localization of the species by GPS, and taxonomic identification. The ethnobotanical information was acquired through a bibliographic survey. Botanical, geographic, and ethnobotanical information was filed and included in floristic maps produced using the free Open Source QGIS software. Of the total wild species surveyed, 50% were halophytes with ethnobotanical properties, with a predominance for medicinal ones. Some of them are the subject of numerous studies today such as those on antioxidants. Both investigated areas showed a high floristic and ethnobotanical value. This makes them interesting as potential germplasm banks to be used in various application contexts of ethnobotany.
Strategies to Maximize Kernel Processing in a Brazilian Vitreous Endosperm Hybrid
Authors: Salvati Gustavo Gonçalves de Souza , Santos Willian Pereira dos , Carvalho-Estrada Paula de Almeida , Morais Greiciele de , Salvatte Jéssica Mariane Silveira , Fachin Lorena , Arthur Bruno Augusto Valverde , Ribeiro Ariádna Patricia , Morais Júnior Nilson Nunes , Oliveira Késia da Silva , Nussio Luiz Gustavo
Published: February 2023

Whole-plant corn silage (WPCS) is a major source of forage for dairy cattle in Brazil. Improved kernel processing may be especially advantageous when feeding corn hybrids with vitreous endosperms. Two experiments were conducted to evaluate the effects of the theoretical length of cut (TLOC) and ensiling time on WPCS particle size and kernel processing with two types of forage harvesters. In the first trial, the plant was harvested by a pull-type forage harvester (PTFH) at TLOCs of 3, 6 and 9 mm. In the second trial, the harvesting was performed by a self-propelled forage harvester (SPFH) at TLOCs of 6, 12 and 18 mm, with a roll gap of 3 mm. The WPCS were stored for 0, 35 and 140 days. In the PTFH trial, the TLOCs of 3 and 6 mm did not affect the WPCS particle size distribution and mean particle length (MPL). However, the TLOC of 9 mm increased the MPL. The increase in the TLOC with the SPFH led to a higher MPL and percentage of long particles. The ensiling time increased the MPL and long particles only for the WPCS harvested by the SPFH. The strategy of reducing the TLOC with the SPFH increased the percentage of kernels smaller than 4.75 mm. Furthermore, the TLOC of 6 mm led to the best kernel processing with the SPFH. The ensiling time reduced the particle size of the kernel fractions for both forage harvesters. The corn silage processing score was improved with 140 days of ensiling with the SPFH. These findings suggest that increasing the ensiling time and a low TLOC in SPFHs (6 mm) may be good strategies to increase kernel damage and starch digestibility in WPCS.
Phenotypic Variability for Root Traits in Andean Common Beans Grown with and without Aluminum Stress Conditions
Authors: Ambachew Daniel , Asfaw Asrat , Blair Matthew W.
Published: February 2023

Genetic variation in wild relatives, GenBank accessions, landraces, and cultivars can unlock key alleles for the traits of interest for breeding programs. Breeding programs often utilize different strategies to quantify the source of heritable variation for target traits. One neglected area of study is the root traits of diverse genotypes, and this is especially the case for aluminum toxicity effects on legumes such as the common bean, which is the most used pulse for direct human consumption. This study evaluated 267 genotypes of common bean that were part of the global Andean Diversity Panel (ADP), consisting mainly of genotypes assembled from public and private breeding programs in Africa and North America, as well as elite lines and land races from the USDA. The ADP was evaluated for root traits at the seedling stage in the Tennessee State University (TSU) greenhouse using a hydroponic system with a standard nutrient solution with and without aluminum (Al). The recorded data on the roots per trial were fit to a linear mixed model for the analysis of variance in order to test for the genotype differences. Adjusted means considered replication and blocks within replication as random effects and genotypes as fixed effects. These were then used for Pearson correlation tests and for principal component analysis (PCA), where the first two vectors accounted for 94.5% and 93% of the explained variation under the control and Al-treatment conditions, respectively. Genotypes were clustered based on the morphology of roots in response to Al-toxicity treatment using the Euclidean distance and Ward’s hierarchical agglomerative clustering method, identifying four distinctive groups significant at p < 0.01. The intra-cluster distance was lower than the inter-cluster distances, which indicated a heterogeneous and homogeneous nature between and within clusters, respectively. The results suggest that crossing between accessions from two of the clusters would result in the maximum genetic segregation. One cluster was found to have a higher Al-toxicity tolerance than the others.
Combined Effects of Cytokinin and UV-C Light on Phenolic Pattern in Ceratonia siliqua Shoot Cultures
Authors: Costa-Pérez Antonio , Ferrer María A. , Calderón Antonio A.
Published: February 2023

Carob (Ceratonia siliqua L.) is an underutilized traditional crop in the Mediterranean regions that has gained much interest due to its high nutritional traits and resilience to drought and salinity. However, conventional methods of carob propagation are not enough to meet its increasing market demands. The present study analyzes to what extent benzyl adenine (BA) treatments (0.1, 0.5 and 1.0 mg L−1) alone or in combination with UV-C irradiation (3.34 and 10.01 kJ m−2) affect the antioxidant capacity and the levels of phenolic compounds in in vitro carob shoot cultures as well as their performance in terms of the content of photosynthetic pigments and sugars. Results showed that the combination of both treatments resulted in an increased content of chlorophylls, carotenoids, and sugars, particularly at 0.5 mg L−1 BA and the highest UV-C dose. Antioxidant capacity, assessed by the DPPH method, and the levels of soluble flavonoids, flavones and flavonols, and hydroxycinnamic acids were highly influenced by the interaction between BA and UV-C in the combined treatments. This indicates a clear dependence on BA concentration in the response of carob in vitro shoots to UV-C. This could be the basis for the implementation of more efficient carob micropropagation processes.
Economic and Environmental Sustainability Assessment of an Innovative Organic Broccoli Production Pattern
Authors: Scuderi Alessandro , Timpanaro Giuseppe , Branca Ferdinando , Cammarata Mariarita
Published: February 2023

Organic farming nowadays is held up as a model of sustainability; however, this is not always an economic advantage for farmers due to the reduced yields compared to the conventional regime. The aim of the study is therefore to provide an environmental and economic analysis of the innovative organic model proposed by the Bresov project to assess its sustainability. The study is therefore based on a Life Cycle Assessment methodology and the economic evaluation, through the calculation of the gross income of innovative organic and conventional broccoli cultivation in Sicily. The impact categories analysed reported a 60–100% reduction in impact in the case of innovative organic compared to conventional. From an economic point of view, although there is a minimal reduction in yield in organic compared to conventional, there is an increase in production costs that translates into a reduction in the gross income of approximately 61%. These gaps are filled when the organic product is granted a premium price and thanks to aid from the Common Agricultural Policy. The innovative organic approach, characterised by new products and soil management methods, confirms it as an alternative to conventional. This approach contrasts with the mere substitution of synthetic products.
Seroprevalence and Risk Factors for Bovine Coronavirus Infection among Dairy Cattle and Water Buffalo in Campania Region, Southern Italy
Authors: Ferrara Gianmarco , Iovane Valentina , Improda Elvira , Iovane Giuseppe , Pagnini Ugo , Montagnaro Serena
Published: February 2023

Cattle and water buffalo are the main livestock species that are raised in the Campania region, southern Italy, and they contribute significantly to the regional rural economy. Currently there are limited data on the prevalence of relevant impact infections, such as bovine coronavirus (BCov), an RNA virus that causes acute enteric and respiratory disease. Although these diseases are described primarily in cattle, there have been reports of spillovers to other ruminants, including water buffalo. Here, we determined the seroprevalence of BCoV in cattle and water buffalo in the Campania region of southern Italy. An overall seroprevalence of 30.8% was determined after testing 720 sampled animals with a commercial enzyme-linked immunosorbent assay. A risk factor analysis revealed that the seropositivity rates in cattle (49.2%) were higher than in water buffalo (5.3%). In addition, higher seroprevalence rates were observed in older and purchased animals. In cattle, housing type and location were not associated with higher seroprevalence. The presence of BCoV antibodies in water buffalo was associated with the practice of co-inhabiting with cattle, demonstrating that this practice is incorrect and promotes the transmission of pathogens between different species. Our study found a considerable seroprevalence, which is consistent with previous research from other countries. Our results provide information on the widespread distribution of this pathogen as well as the risk factors that are involved in its transmission. This information could be useful in the control and surveillance of this infection.
Fetal Growth and Osteogenesis Dynamics during Early Development in the Ovine Species
Authors: Succu Sara , Contu Efisiangelo , Bebbere Daniela , Gadau Sergio Domenico , Falchi Laura , Nieddu Stefano Mario , Ledda Sergio
Published: February 2023

Increased knowledge of the developmental processes during gestation could provide valuable information on potential alterations in embryonic/fetal development. We examined the development of ovine conceptus between the 20th and 70th day of gestation with three convergent analyses: (1) uterus ultrasound examination and measurement (eco) of crown–rump length (CRL) and biparietal diameter (BPD) of the conceptus; (2) direct measurement (vivo) of CRL and BPD of the conceptus outside the uterus (3) osteo–cartilage dynamics during development by differential staining. No significant differences were observed between eco and vivo measurements for CRL and BPD in all examined concepti. CRL and BPD, instead, showed a significant positive linear correlation with gestational age. The study of osteogenesis dynamics has demonstrated a completely cartilaginous ovine fetus at up to 35 days of gestation. The ossification begins in the skull (40th day) and is almost complete between the 65th and the 70th of pregnancy. Our study highlighted that CRL and BPD are accurate parameters for gestational age estimation in the first part of sheep pregnancy and provides an overview of osteochondral temporal dynamics. Furthermore, tibia ossification is a valid parameter to estimate fetal age by ultrasound.
Assistance Dogs for People with Younger (Early)-Onset Dementia: The Family Carer’s Experience
Authors: Marks Genée , McVilly Keith R.
Published: February 2023

There is growing evidence for trained assistance dogs promoting the health, wellbeing, and quality of life of people in a variety of circumstances, including for those with dementia. Little is known about people with younger (early)-onset dementia (YOD) and family carers. As part of a larger study involving 14 people with YOD matched with trained assistance dogs over a two-year period, we report analyses of interviews with 10 family carers conducted on multiple occasions investigating their experience with an assistance dog. Interviews were recorded, transcribed and subjected to inductive thematic analysis. They told a range of experiences; the good and the challenging. Findings fell into three areas: the human–animal bond; relationship dynamics; and responsibility for caring. Concerns were raised with respect to the resources required of carers together with the financial resources needed to support an assistance dog. The study concludes that trained assistance dogs can play an important role promoting the health and wellbeing of both people with YOD and of their family carers. However, support needs to be in place as the circumstances of the family member with YOD changes and the role of the assistance dog as part of the family also changes. Practical (financial) support of a scheme such as the Australian National Disability Insurance Scheme (NDIS) could be important to sustaining such support.
Precision Livestock Farming: What Does It Contain and What Are the Perspectives?
Authors: Kleen Joachim Lübbo , Guatteo Raphaël
Published: February 2023

Precision Livestock Farming (PLF) describes the combined use of sensor technology, the related algorithms, interfaces, and applications in animal husbandry. PLF technology is used in all animal production systems and most extensively described in dairy farming. PLF is developing rapidly and is moving beyond health alarms towards an integrated decision-making system. It includes animal sensor and production data but also external data. Various applications have been proposed or are available commercially, only a part of which has been evaluated scientifically; the actual impact on animal health, production and welfare therefore remains largely unknown. Although some technology has been widely implemented (e.g., estrus detection and calving detection), other systems are adopted more slowly. PLF offers opportunities for the dairy sector through early disease detection, capturing animal-related information more objectively and consistently, predicting risks for animal health and welfare, increasing the efficiency of animal production and objectively determining animal affective states. Risks of increasing PLF usage include the dependency on the technology, changes in the human–animal relationship and changes in the public perception of dairy farming. Veterinarians will be highly affected by PLF in their professional life; they nevertheless must adapt to this and play an active role in further development of technology.
Validation of Methods for Assessment of Dust Levels in Layer Barns
Authors: Mousqué Solène , Mocz Frédérique , Riber Anja B.
Published: February 2023

The dust level is included in the animal welfare legislation of the European Union, implying assessment of dust levels during veterinary welfare inspections. This study aimed to develop a valid and feasible method for measuring dust levels in poultry barns. Dust levels were assessed in 11 layer barns using six methods: light scattering measurement, the dust sheet test with durations of 1 h and 2–3 h, respectively, visibility assessment, deposition assessment, and a tape test. As a reference, gravimetric measurements were obtained – a method known to be accurate but unsuitable for veterinary inspection. The dust sheet test 2–3 h showed the highest correlation with the reference method with the data points scattered closely around the regression line and the slope being highly significant (p = 0.00003). In addition, the dust sheet test 2–3 h had the highest adjusted R2 (0.9192) and the lowest RMSE (0.3553), indicating a high capability of predicting the true concentration value of dust in layer barns. Thus, the dust sheet test with a test duration of 2–3 h is a valid method for assessing dust levels. A major challenge is the test duration as 2–3 h is longer than most veterinary inspections. Nevertheless, results showed that potentially, with some modifications to the scoring scale, the dust sheet test may be reduced to 1 h without losing validity.
Dietary Protein Requirement of Juvenile Dotted Gizzard Shad Konosirus punctatus Based on the Variation of Fish Meal
Authors: Liu Tao , Weng Xinzhi , Wang Jiteng , Han Tao , Wang Yuebin , Chai Xuejun
Published: February 2023

An 8-week feeding trial was conducted to investigate the effects of dietary protein levels on growth performance, feed utilization, and energy retention of juvenile dotted gizzard shad Konosirus punctatus based on the variation of fish meal. Fish meal was used as the sole protein source; five semi-purified diets were formulated with varying crude protein (CP) levels of 22.52%, 28.69%, 34.85%, 38.84%, 45.78% (CP1-CP5 diets). A total of 300 uniform juveniles with initial body weight 3.61 ± 0.20 g fish−1 were randomly divided into five groups with three replicates in each group. The results showed that different CP levels did not significantly affect the survival of juvenile K. punctatus (p > 0.05). The values of weight gain (WG) and specific growth ratio (SGR) showed a general enhancing trend and then weakened with increasing dietary CP levels (p > 0.05). Feed utilization also improved with increasing dietary CP levels (p > 0.05), and the optimal feed conversion ratio (FCR) value was found in fish fed the diet with CP3 (p > 0.05). The rise of dietary CP from 22.52% to 45.78% enhanced the daily feed intake (DFI) and protein efficiency ratio (PER) values of K. punctatus (p < 0.05). With the increase of dietary CP levels, daily nitrogen intake (DNI), energy retention (ER), and lipid retention (LR) elevated, while retention (NR), daily energy intake (DEI), and daily lipid intake (DLI) reduced (p < 0.05). No statistical differences in the content of water, crude protein, and crude lipid were observed among different treatments (p > 0.05). The activity of lipase in CP3 and CP4 diets was significantly higher than that of the CP1 diet (p < 0.05). Fish fed CP2 and CP3 diets had significantly higher amylase activity than that of the CP5 diet (p < 0.05). The levels of alanine aminotransferase (GPT) first enhanced and then decreased as dietary CP levels raised. The second-order polynomial regression model analysis of the WG and FCR indicated that the optimal dietary protein level for K. punctatus is about 31.75–33.82% based on the variation of fish meal.
Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis
Authors: Banfi Cristina , Mallia Alice , Ghilardi Stefania , Brioschi Maura , Gianazza Erica , Eligini Sonia , Sahlén Pelin , Baetta Roberta
Published: February 2023

The process of adipogenesis involves the differentiation of preadipocytes into mature adipocytes. Excessive adipogenesis promotes obesity, a condition that increasingly threatens global health and contributes to the rapid rise of obesity-related diseases. We have recently shown that prenylcysteine oxidase 1 (PCYOX1) is a regulator of atherosclerosis-disease mechanisms, which acts through mechanisms not exclusively related to its pro-oxidant activity. To address the role of PCYOX1 in the adipogenic process, we extended our previous observations confirming that Pcyox1−/−/Apoe−/− mice fed a high-fat diet for 8 or 12 weeks showed significantly lower body weight, when compared to Pcyox1+/+/Apoe−/− mice, due to an evident reduction in visceral adipose content. We herein assessed the role of PCYOX1 in adipogenesis. Here, we found that PCYOX1 is expressed in adipose tissue, and, independently from its pro-oxidant enzymatic activity, is critical for adipogenesis. Pcyox1 gene silencing completely prevented the differentiation of 3T3-L1 preadipocytes, by acting as an upstream regulator of several key players, such as FABP4, PPARγ, C/EBPα. Proteomic analysis, performed by quantitative label-free mass spectrometry, further strengthened the role of PCYOX1 in adipogenesis by expanding the list of its downstream targets. Finally, the absence of Pcyox1 reduces the inflammatory markers in adipose tissue. These findings render PCYOX1 a novel adipogenic factor with possible pathophysiological or therapeutic potential.
Microbiota, Oxidative Stress, and Skin Cancer: An Unexpected Triangle
Authors: Azzimonti Barbara , Ballacchino Chiara , Zanetta Paola , Cucci Marie Angele , Monge Chiara , Grattarola Margherita , Dianzani Chiara , Barrera Giuseppina , Pizzimenti Stefania
Published: February 2023

Mounting evidence indicates that the microbiota, the unique combination of micro-organisms residing in a specific environment, plays an essential role in the development of a wide range of human diseases, including skin cancer. Moreover, a persistent imbalance of microbial community, named dysbiosis, can also be associated with oxidative stress, a well-known emerging force involved in the pathogenesis of several human diseases, including cutaneous malignancies. Although their interplay has been somewhat suggested, the connection between microbiota, oxidative stress, and skin cancer is a largely unexplored field. In the present review, we discuss the current knowledge on these topics, suggesting potential therapeutic strategies.
Oxidative Stress and Inflammation in Acute and Chronic Lung Injuries
Authors: Bezerra Frank Silva , Lanzetti Manuella , Nesi Renata Tiscoski , Nagato Akinori Cardozo , Silva Cyntia Pecli e , Kennedy-Feitosa Emanuel , Melo Adriana Correa , Cattani-Cavalieri Isabella , Porto Luís Cristóvão , Valenca Samuel Santos
Published: February 2023

Acute and chronic lung injuries are among the leading causes of mortality worldwide. Lung injury can affect several components of the respiratory system, including the airways, parenchyma, and pulmonary vasculature. Although acute and chronic lung injuries represent an enormous economic and clinical burden, currently available therapies primarily focus on alleviating disease symptoms rather than reversing and/or preventing lung pathology. Moreover, some supportive interventions, such as oxygen and mechanical ventilation, can lead to (further) deterioration of lung function and even the development of permanent injuries. Lastly, sepsis, which can originate extrapulmonary or in the respiratory system itself, contributes to many cases of lung-associated deaths. Considering these challenges, we aim to summarize molecular and cellular mechanisms, with a particular focus on airway inflammation and oxidative stress that lead to the characteristic pathophysiology of acute and chronic lung injuries. In addition, we will highlight the limitations of current therapeutic strategies and explore new antioxidant-based drug options that could potentially be effective in managing acute and chronic lung injuries.
Modeling and Optimization of Phenolic Compounds from Sage (Salvia fruticosa L.) Post-Distillation Residues: Ultrasound- versus Microwave-Assisted Extraction
Authors: Irakli Maria , Bouloumpasi Elisavet , Christaki Stamatia , Skendi Adriana , Chatzopoulou Paschalina
Published: February 2023

The essential oil production of Salvia fruticosa L. generates considerable amounts of post-distillation solid residues (PRES) which are rich in phenolic compounds. In the present work, the recovery of phenolic antioxidants from PRES by using Microwave-Assisted Extraction (MAE) and Ultrasound-Assisted Extraction (UAE) were separately optimized, according to the Box–Behnken experimental design. The optimization was based on extraction yield, total phenolic content (TPC), total flavonoid content (TFC), rosmarinic acid (RMA), carnosol (CARO), carnosic acid (CARA), and antioxidant activity. The optimal processing parameters were 72% and 68% ethanol, a 15- and 10-min extraction time, a 40 °C and 47 °C extraction temperature, and a 1:30 and 1:10 solid-to-solvent ratio, for MAE and UAE, respectively. Results showed that the levels of RMA, CARO, and CARA in UAE extracts were influenced mainly by ethanol concentration, extraction time, and extraction temperature, while MAE extracts were only influenced by the first two factors. Experimenting with the optimal conditions revealed MAE as more effective than UAE in the recovery of RMA and CARA. The experimental values were in good agreement with the predicted ones, indicating model efficacy in MAE and UAE optimization to effectively extract phenolic compounds from PRES for their further application in food and pharmaceutical industries.
Withania somnifera L.: Phenolic Compounds Composition and Biological Activity of Commercial Samples and Its Aqueous and Hydromethanolic Extracts
Authors: Polumackanycz Milena , Petropoulos Spyridon A. , Śledziński Tomasz , Goyke Elżbieta , Konopacka Agnieszka , Plenis Alina , Viapiana Agnieszka
Published: February 2023

In the present study, the chemical composition and bioactive properties of commercially available Withania somnifera samples were evaluated. The hydromethanolic and aqueous extracts of the tested samples were analyzed in terms of phenolic compound composition, ascorbic acid content, antioxidant and antibacterial activity, and acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities. Polyphenols and ascorbic acid content, as well as the antioxidant activity, were higher in the aqueous extracts than in the hydromethanolic extracts. Generally, aqueous extracts presented higher antioxidant activity than the hydromethanolic ones, especially in the case of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. Moreover, higher amounts of phenolic acids and flavonoids were found in the hydromethanolic extracts compared to the aqueous ones. Regarding the antibacterial properties, samples 4, 6, and 10 showed the best overall performance with growth-inhibitory activities against all the examined bacteria strains. Finally, the aqueous and hydromethanolic extracts were the most efficient extracts in terms of AChE and BChE inhibitory activities, respectively. In conclusion, our results indicate that W. somnifera possesses important bioactive properties which could be attributed to the high amounts of phenolic compounds. However, a great variability was recorded in commercially available products, suggesting significant differences in the origin of product and the processing method.