Oxygen depletion in FLASH ultra‐high‐dose‐rate radiotherapy: A molecular dynamics simulation
Authors: Abolfath Ramin , Grosshans David , Mohan Radhe 
Published: October 2020

Purpose

We present a first‐principles molecular dynamics (MD) simulation and expound upon a mechanism of oxygen depletion hypothesis to explain the mitigation of normal tissue injury observed in ultra‐high‐dose‐rate (UHDR) FLASH radiotherapy.

Methods

We simulated damage to a segment of DNA (also representing other biomolecules such as RNA and proteins) in a simulation box filled with O and molecules. Attoseconds physical interactions (ionizations, electronic, and vibrational excitations) were simulated by using the Monte Carlo track structure code Geant4‐DNA. Immediately after ionization, ab initio Car–Parrinello molecular dynamics (CPMD) simulation was used to identify which O and molecules surrounding the DNA molecule were converted into reactive oxygen species (ROS). Subsequently, the femto‐ to nanosecond reactions of ROS were simulated by using MD with reactive force field (ReaxFF), to illustrate ROS merging into new types of non‐reactive oxygen species (NROS) due to strong coupling among ROS. A coarse‐grained model was constructed to describe the relevant collective phenomenon at the macroscopic level on ROS aggregation and formation of NROS agglomerates consistent with the underlying microscopic pathways obtained from MD simulations.

Results

Time‐dependent molecular simulations revealed the formation of metastable and transient spaghetti‐like complexes among ROS generated at UHDR. At the higher ROS densities produced under UHDR, stranded chains (i.e., NROS) are produced, mediated through attractive electric polarity forces, hydrogen bonds, and magnetic dipole–dipole interactions among hydroxyl radicals. NROS tend to be less mobile than cellular biomolecules as opposed to the isolated and sparsely dense ROS generated at conventional dose rates (CDR). We attribute this effect to the suppression of biomolecular damage induced per particle track. At a given oxygen level, as the dose rate increases, the size and number of NROS chains increase, and correspondingly the population of toxic ROS components decreases. Similarly, at a given high dose rate, as the oxygen level increases, so do the size and number of NROS chains until an optimum level of oxygen is reached. Beyond that level, the amount of oxygen present may be sufficient to saturate the production of NROS chains, thereby reversing the sparing effects of UHDRs.

Conclusions

We showed that oxygen depletion, hypothesized to lead to lower normal‐tissue toxicity at FLASH dose rates, takes place within femto‐ to nanoseconds after irradiation. The mechanism is governed by the slow dynamics of chains of ROS complexes (NROS). Under physoxic (≈ 4–5% oxygen) conditions (i.e., in normal tissues), NROS are more abundant than in hypoxic conditions (e.g., <0.3% in parts of tumors), suggesting that biomolecular damage would be reduced in an environment with physoxic oxygen levels. Hence irradiation at UHDRs would be more effective for sparing physoxic normal tissues but not tumors containing regions of hypoxia. At much higher levels of oxygen (e.g., >10–15%), oxygen depletion by UHDRs may not be sufficient for tissue sparing.