bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Simulation and Experimental Validation of a Kaplan Prototype Turbine Operating on a Cam Curve
Authors: Iovănel Raluca Gabriela , Dehkharqani Arash Soltani , Bucur Diana Maria , Cervantes Michel Jose
Published: June 2022

The role of hydropower has become increasingly essential following the introduction of intermittent renewable energies. Quickly regulating power is needed, and the transient operations of hydropower plants have consequently become more frequent. Large pressure fluctuations occur during transient operations, leading to the premature fatigue and wear of hydraulic turbines. Investigations of the transient flow phenomena developed in small-scale turbine models are useful and accessible but limited. On the other hand, experimental and numerical studies of full-scale large turbines are challenging due to production losses, large scales, high Reynolds numbers, and computational demands. In the present work, the operation of a 10 MW Kaplan prototype turbine was modelled for two operating points on a propeller curve corresponding to the best efficiency point and part-load conditions. First, an analysis of the possible means of reducing the model complexity is presented. The influence of the boundary conditions, runner blade clearance, blade geometry and mesh size on the numerical results is discussed. Secondly, the results of the numerical simulations are presented and compared to experimental measurements performed on the prototype in order to validate the numerical model. The mean torque and pressure values were reasonably predicted at both operating points with the simplified model. An analysis of the pressure fluctuations at part load demonstrated that the numerical simulation captured the rotating vortex rope developed in the draft tube. The frequencies of the rotating and plunging components of the rotating vortex were accurately captured, but the amplitudes were underestimated compared to the experimental data.
Investigations of the Thermal Parameters of Hybrid Sol–Gel Coatings Using Nondestructive Photothermal Techniques
Authors: Chrobak Łukasz , Korte Dorota , Budasheva Hanna , Maliński Mirosław , Rodič Peter , Milošev Ingrid , Janta-Lipińska Sylwia
Published: June 2022

This article presents the results of comparative investigations of thermal parameters of hybrid sol–gel coatings (named TMZ) prepared from tetraethyl orthosilicate and organically modified 3-methacryloxypropyltrimethoxysilane. The coatings were prepared with the addition of zirconium(IV) tetrapropoxide chelated with methacrylic acid. Two series of samples were investigated: the first series, TMZ-I, TMZ-II and TMZ-III, with different amounts of zirconium, and the second series, TMZ-I/Ce, TMZ-II/Ce and TMZ-III/Ce, with the addition of cerium nitrate. The influence of the amount of zirconium and cerium on the thermal parameters of the sol–gel coatings was next analyzed. Two non-destructive and photothermal techniques were used for this purpose: photothermal radiometry (PTR) and beam deflection spectroscopy (BDS). The thermal diffusivity and conductivity of the coatings were extracted from the frequency experiments and are presented and discussed. The two-layer model was applied to interpret the photothermal spectra. The results obtained using these two techniques are compared and discussed.
Analytical Minimization of Interior Permanent Magnet Machine Torque Pulsations by Design of Sculpted Rotor
Authors: Hayslett Steven , Pham Thang , Strangas Elias
Published: June 2022

A new and efficient analytical optimization methodology for the design of rotor features is developed and used in interior permanent magnet motors (IPM). The analytical methodology is based on an extended winding function theory to include the IPM rotor’s primary and secondary reluctance paths and the non-homogeneous airgap of the rotor sculpt features. The shape and placement of the rotor features, derived from the analytical-based optimization process, show the improvement in torque average and torque ripple of the IPM machine at a fraction of computational effort. The analytical optimization results are validated with finite element analysis via an exhaustive search.
Slope Estimation Method of Electric Vehicles Based on Improved Sage–Husa Adaptive Kalman Filter
Authors: Guo Jiawei , He Chao , Li Jiaqiang , Wei Heng
Published: June 2022

In order to deal with many influence factors of electric vehicles in driving under complex conditions, this paper establishes the system state equation based on the longitudinal dynamics equation of vehicle. Combined with the improved Sage–Husa adaptive Kalman filter algorithm, the road slope estimation model is established. After the driving speed and rough slope observation are input into the slope estimation model, the accurate road slope estimation at the current time can be obtained. The road slope estimation method is compared with the original Sage–Husa adaptive Kalman filter road slope estimation method through three groups of road tests in different slope ranges, and the accuracy and stability advantages of the proposed algorithm in road conditions with large slopes are verified.
Parametric Transient Stability Constrained Optimal Power Flow Solved by Polynomial Approximation Based on the Stochastic Collocation Method
Authors: Xia Bingqing , Wu Hao , Yang Wenbin , Cao Lu , Song Yonghua
Published: June 2022

To better respond to the impact of power system-uncertain parameters on transient stability, a novel model named the parametric transient stability constrained optimal power flow (parametric TSCOPF) is proposed. It seeks the optimal control scheme of transient stability constrained optimal power flow (TSCOPF) expressed by the function of uncertain parameters in power systems. The key difficulty to solve this model lies in that the relationship between the parametric TSCOPF solution and uncertain parameters is implicit, which is hard to derive generally. To this end, this paper approximates the optimal solution of parametric TSCOPF by polynomial expressions of uncertain parameters based on the stochastic collocation method. First, the parametric TSCOPF model includes both uncertain parameters and transient stability constraints, in which the transient stability constraint is constructed as a set of polynomial expressions using the SCM. Then, to derive the relationship between the parametric TSCOPF solution and uncertain parameters, the SCM is applied to the parametric Karush–Kuhn–Tucker (KKT) conditions of the parametric TSCOPF model, so that the optimal solution of the parametric TSCOPF is approximated by using polynomial expressions with respect to uncertain parameters. The proposed parametric TSCOPF model has been tested on a 3-machine, 9-bus system, and the IEEE 145-bus system, which verifies the effectiveness of the proposed method.
Visualization and Measurement of Swirling Flow of Dry Ice Particles in Cyclone Separator-Sublimator
Authors: Yamasaki Haruhiko , Wakimoto Hiroyuki , Kamimura Takeshi , Hattori Kazuhiro , Nekså Petter , Yamaguchi Hiroshi
Published: June 2022

The dry ice sublimation process of carbon dioxide (CO2) is a unique, environmentally friendly technology that can achieve a temperature of −56 °C or lower, which is a triple point of CO2 in CO2 refrigeration systems. In this study, a cyclone separator-evaporator was proposed to separate dry ice particles in an evaporator. As an initial step before introducing the cyclone separator-evaporator into an actual refrigeration system, a prototype cyclone separator-evaporator was constructed to visualize dry ice particles in a separation chamber. A high-speed camera was used to visualize the non-uniform flow of dry ice particles that repeatedly coalescence and collision in a swirl section. Consequently, the dry ice particle size and the circumferential and axial velocities of dry ice were measured. The results show that the equivalent diameter of the most abundant dry ice particles in the cyclone separation chamber is 2.0 mm. As the inner diameter of the separation section decreases, dry ice particles coalesce and grow from an equivalent diameter of 4 mm to a maximum of 40 mm. In addition, the comparison of the experimental and simulation results shows that the drag force due to CO2 gas flow is dominant in the circumferential velocity of dry ice particles.
Evidence for Alternate Stable States in an Ecuadorian Andean Cloud Forest
Authors: Mariscal Ana , Thomas Daniel Churchill , Haffenden Austin , Manobanda Rocío , Defas William , Angel Chinchero Miguel , Simba Larco José Danilo , Jaramillo Edison , Roy Bitty A. , Peck Mika
Published: June 2022

Tree diversity inventories were undertaken. The goal of this study was to understand changes in tree community dynamics that may result from common anthropogenic disturbances at the Reserva Los Cedros, a tropical montane cloud forest reserve in northern Andean Ecuador. The reserve shows extremely high alpha and beta tree diversity. We found that all primary forest sites, regardless of age of natural gaps, are quite ecologically resilient, appearing to return to a primary-forest-type community of trees following gap formation. In contrast, forests regenerating from anthropogenic disturbance appear to have multiple possible ecological states. Where anthropogenic disturbance was intense, novel tree communities appear to be assembling, with no indication of return to a primary forest state. Even in ancient primary forests, new forest types may be forming, as we found that seedling community composition did not resemble adult tree communities. We also suggest small watersheds as a useful basic spatial unit for understanding biodiversity patterns in the tropical Andes that confound more traditional Euclidean distance as a basic proxy of dissimilarity. Finally, we highlight the conservation value of Reserva Los Cedros, which has managed to reverse deforestation within its boundaries despite a general trend of extensive deforestation in the surrounding region, to protect a large, contiguous area of highly endangered Andean primary cloud forest.
Acid Hydrolysable Components Released from Four Decomposing Litter in an Alpine Forest in Sichuan, China
Authors: Liao Shu , Yue Kai , Ni Xiangyin , Wu Fuzhong
Published: June 2022

Acid hydrolysable components have been thought to release from plant litter at early periods of decomposition and to be sensitive to hydrological change. Variations in snow depth and timing may alter the release of acid hydrolysable components from decomposing litter in seasonally snow-covered ecosystems. Here, we measured the release of acid hydrolyzable components from four foliar litters (fir, cypress, larch and birch) in deep and shallow snow plots during winter (snow formation, snow coverage and snowmelt stages) and growing seasons in an alpine forest from 2012 to 2016. We found that the content of acid hydrolysable components was 16–21% in fresh litter across species, and only 4–5% of these components remained in the litter after four years of decomposition when 53–66% of litter mass was lost. The content of acid hydrolysable components greatly decreased within 41 days and during the growing seasons of the fourth year of decomposition, suggesting that acid hydrolysable components in plant litter are not only released at early periods but also at a very late period during litter decay. However, the content of acid hydrolysable components increased significantly at snowmelt stages. Reduced snow cover increased the content and remaining level of acid hydrolysable components during the four years of decomposition by altering leaching, microbial biomass and stoichiometry. We propose that more effective partitioning of chemical fractions should be incorporated to distinguish the carbon and nutrient release during litter decomposition within a complex context of the changing environment.
Long-Term Response of Fuel to Mechanical Mastication in South-Eastern Australia
Authors: Pickering Bianca J. , Burton Jamie E. , Penman Trent D. , Grant Madeleine A. , Cawson Jane G.
Published: June 2022

Mechanical mastication is a fuel management strategy that modifies vegetation structure to reduce the impact of wildfire. Although past research has quantified immediate changes to fuel post-mastication, few studies consider longer-term fuel trajectories and climatic drivers of this change. Our study sought to quantify changes to fuel loads and structure over time following mastication and as a function of landscape aridity. Measurements were made at 63 sites in Victoria, Australia. All sites had been masticated within the previous 9 years to remove over-abundant shrubs and small trees. We used generalised additive models to explore trends over time and along an aridity gradient. Surface fuel loads were highest immediately post-mastication and in the most arid sites. The surface fine fuel load declined over time, whereas the surface coarse fuel load remained high; these trends occurred irrespective of landscape aridity. Standing fuel (understorey and midstorey vegetation) regenerated consistently, but shrub cover was still substantially low at 9 years post-mastication. Fire managers need to consider the trade-off between a persistently higher surface coarse fuel load and reduced shrub cover to evaluate the efficacy of mastication for fuel management. Coarse fuel may increase soil heating and smoke emissions, but less shrub cover will likely moderate fire behaviour.
Suppression of Vortex-Induced Vibration and Phase-Averaged Analysis of the Wake Generated by a Circular Cylinder Covered with Helical Grooves
Authors: Hao Zhiyong , Sun Chenlin , Lu Yucen , Bi Kaiming , Zhou Tongming
Published: June 2022

The effect of triple helical grooves on the suppression of vortex-induced vibration (VIV) of a circular cylinder was investigated experimentally in a wind tunnel over Reynolds number in the range of 1 × 104 < Re < 4 × 104. It was found that the helical grooves were effective in suppressing VIV with the peak amplitude reduction of approximately 36%. In addition, the lock-on region was also reduced. To explore the mechanism for the suppression of VIV, experiments on flow structures for a stationary grooved cylinder were also conducted in a wind tunnel at a free stream velocity U∞ of 4.37 m/s, corresponding to a Reynolds number based on the bare cylinder diameter of about 3500. The data were then analyzed using the phase-averaged method to evaluate the coherent vortex structures in the wakes. The results for the stationary grooved cylinder showed that the grooves weakened vortex shedding in the near wake. In addition, the grooves also reduced the drag coefficient by 6.6%. These results help explain the reduction of VIV using helical grooves.
Bioprocessing of Wheat and Amaranth Bran for the Reduction of Fructan Levels and Application in 3D-Printed Snacks
Authors: Habuš Matea , Mykolenko Svitlana , Iveković Sofija , Pastor Kristian , Kojić Jovana , Drakula Saša , Ćurić Duška , Novotni Dubravka
Published: June 2022

Bran can enrich snacks with dietary fibre but contains fructans that trigger symptoms in people with irritable bowel syndrome (IBS). This study aimed to investigate the bioprocessing of wheat and amaranth bran for degrading fructans and its application (at 20% flour-based) in 3D-printed snacks. Bran was bioprocessed with Saccharomyces cerevisiae alone or combined with inulinase, Kluyveromyces marxianus, Limosilactobacillus fermentum, or commercial starter LV1 for 24 h. Fructans, fructose, glucose, and mannitol in the bran were analysed enzymatically. Dough rheology, snack printing precision, shrinkage in baking, texture, colour, and sensory attributes were determined. The fructan content of wheat bran was 2.64% dry weight, and in amaranth bran, it was 0.96% dry weight. Bioprocessing reduced fructan content (up to 93%) depending on the bran type and bioprocessing agent, while fructose and mannitol remained below the cut-off value for IBS patients. Bran bioprocessing increased the complex viscosity and yield stress of dough (by up to 43 and 183%, respectively) in addition to printing precision (by up to 13%), while it lessened shrinkage in baking (by 20–69%) and the hardness of the snacks (by 20%). The intensity of snack sensory attributes depended on the bran type and bioprocessing agent, but the liking (“neither like nor dislike”) was similar between samples. In conclusion, snacks can be enriched with fibre while remaining low in fructans by applying bioprocessed wheat or amaranth bran and 3D printing.
Can the Introduction of Different Olive Cakes Affect the Carcass, Meat and Fat Quality of Bísaro Pork?
Authors: Leite Ana , Domínguez Rubén , Vasconcelos Lia , Ferreira Iasmin , Pereira Etelvina , Pinheiro Victor , Outor-Monteiro Divanildo , Rodrigues Sandra , Lorenzo José Manuel , Santos Eva María , Andrés Silvina Cecilia , Campagnol Paulo C. B. , Teixeira Alfredo
Published: June 2022

The present study aimed to evaluate the effect of the inclusion of different olive cakes in the diet of Bísaro pigs on the carcass, meat and fat. The carcasses of 40 animals fed a diet with five treatments (T1—Basic diet and commercial feed; T2—Basic diet + 10% crude olive cake; T3—Basic diet + 10% olive cake, two phases; T4—Basic diet + 10% exhausted olive cake; T5—Basic diet + 10% exhausted olive cake + 1% olive oil) were used to study the effect on carcass traits, physicochemical meat quality and lipid composition of meat and backfat. There were no significant differences between treatments for the conformation measurements performed, except for the length at the seventh and last rib (p < 0.05). The percentage of prime cuts of the carcass in Bísaro pig is within the values indicated by the Portuguese Standard 2931. No significant differences between treatments for body weight, pH and carcass weight were found. The values of ultimate pH (5.7), L* (51–52), b* (11–12) and SF (3.4–4.2) observed confirm a non-exudative and firm meat without quality deviations, such as DFD or PSE. Thus, as a general conclusion, the inclusion of different olive cakes in the diet of Bísaro pigs did not cause any negative consequences on the carcass characteristics and conformation as well as in the meat and lipidic quality. In addition, the inclusion of this olive industry by-product in the animal diet would be an important contribution to solving the problem of the great environmental impact from olive-mill wastewaters from the extractive industries.
Black Bean Pasta Meals with Varying Protein Concentrations Reduce Postprandial Glycemia and Insulinemia Similarly Compared to White Bread Control in Adults
Authors: Winham Donna M. , Thompson Sharon V. , Heer Michelle M. , Davitt Elizabeth D. , Hooper Sharon D. , Cichy Karen A. , Knoblauch Simon T.
Published: June 2022

Postprandial glycemic and insulinemic effects of three black bean pastas were evaluated among eighteen normoglycemic adults (8 men, 10 women) in a randomized crossover trial. Black beans were milled into flour using a commercial Knife or compression/decompression mill (C/D mill). The C/D-mill-derived pastas had medium protein (Combo-MP) and low protein (Cyclone-LP) concentrations. Three black bean flour pastas (Knife, Combo-MP, and Cyclone-LP) were compared to two controls: white bread and whole black beans. Treatments contained 50 g of available carbohydrate. Plasma glucose, serum insulin, and appetite measures were collected at fasting and 30, 60, 90, 150, and 180 min postprandial. Gastrointestinal symptoms were evaluated 10–12 h postprandial. ANOVA (one-way, repeated measures) was used to evaluate satiety, gastrointestinal symptoms, sensory variables, glucose and insulin differences from baseline, and incremental area under the curve (iAUC) by time and/or treatment. Three-hour glucose and insulin iAUCs were lower with whole black beans than white bread. Black bean pasta meals increased satiety, reduced appetite, and produced numerically lower glucose and insulin responses than white bread. However, no differences were observed between pasta types, indicating a similar metabolic response regardless of milling technique. Our results provide evidence for dietary guidance to reduce postprandial glucose and related health risks through pulse food products.
Effect of Microwave Treatment on Adzuki Beans (Vigna angularis L.) under Dry State—Analyzing Microstructure, Water Absorption, and Antioxidant Properties
Authors: Oh Seon-Min , Song Seok-Bo , Lee Jeom-Sig , Oh You-Geun , Choi Yu-Chan , Lee Jeong-Heui , Kwak Jieun
Published: June 2022

In this study, a microwave was used on adzuki beans (Arari and Geomguseul) without water, in order to investigate their changes in microstructure, water absorption, and antioxidative properties. As the microwave treatment time increased (2450 MHz, 0 to 60 s), the lightness, redness, and yellowness were reduced, and moisture content significantly decreased in both varieties. The microstructure space between the seed coat, cotyledon, and pores within the cotyledon were observed, due to the loss of moisture. Regardless of microwave treatment, the water absorption behavior of the adzuki beans was sigmoidal. However, the water absorption kinetics of Arari increased after microwave treatment, whereas with microwave-treated Geomguseul, the water absorption rate decreased, compared to the control, except for the sample treated for 30 s. During soaking, the water absorption and softening rates in the microwave-treated adzuki bean were twice as fast as the untreated beans. Antioxidant activity, total phenolic compounds, and total flavonoid compounds were greatly improved by microwave treatment. These results indicate that microwave treatment affects the color, hydration, and bioactive compounds, and it can be used as a pretreatment method before processing adzuki beans.
Supramolecular Rings as Building Blocks for Stimuli-Responsive Materials
Authors: Traeger Hanna , Ghielmetti Alyssa , Sagara Yoshimitsu , Schrettl Stephen , Weder Christoph
Published: June 2022

Stimuli-responsive polymers are of great interest due to their ability to translate changing environmental conditions into responses in defined materials. One possibility to impart such behavior is the incorporation of optically active molecules into a polymer host. Here, we describe how sensor molecules that consist of a π-extended benzothiadiazole emitter and a naphthalene diimide quencher can be exploited in this context. The two optically active entities were connected via different spacers and, thanks to attractive intramolecular interactions between them, the new sensor molecules assembled into cyclic structures in which the fluorescence was quenched by up to 43% when compared to solutions of the individual dyes. Detailed spectroscopic investigations of the sensor molecules in solution show that the extent of donor/acceptor interactions is influenced by various factors, including solvent polarity and ion concentration. The new sensor molecule was covalently incorporated into a polyurethane; the investigation of the optical characteristics in both the solid and solvent-swollen states indicates that a stimulus-induced formation of associated dye pairs is possible in polymeric materials. Indeed, a solvatochromic quenching effect similar to the behavior in solution was observed for solvent-swollen polymer samples, leading to an effective change of the green emission color of the dye to a yellow color.
Use of Betaine-Based Gel and Its Potential Application in Enhanced Oil Recovery
Authors: Wu Yuman , Zhang Jie , Dong Sanbao , Li Yongfei , Slaný Michal , Chen Gang
Published: June 2022

In this paper, a betaine-based gel containing 2.0% erucamide propyl betaine (EAPB), 0.5% oleic acid amide propyl betaine (OAPB), and 0.1% KCl was prepared for use as a fracturing fluid. The performance evaluation showed that KCl may improve the temperature resistance and increase the viscosity of the optimized fracturing fluid. At 80 °C, the apparent viscosity of the viscoelastic surfactant (VES)-based fracturing fluid was approximately 50 mPa·s. Furthermore, the gel had high shear resistance, good viscosity stability, and high sand-carrying performance. After being sheared at 170 s−1 for 60 min, the reduction in viscosity was 13.6%. The viscosity of the gel was relatively stable at room temperature (27 °C) for one week. In a suspension containing 10% sand (particle size < 0.45 mm, density = 2.75 g cm−3), the settling velocity of proppant particles was 1.15 cm h−1. In addition, we detected that the critical micelle concentration of this gel was approximately 0.042 wt%. The viscosity could be reduced to <5 mPa·s at 60 °C within 1 h when 6.0% crude oil was present, and oil displacement experiments showed that the broken fracturing fluid can enhance the oil displacement rate up to 14.5%. This work may facilitate research on fracturing fluids and oil recovery.
Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings
Authors: Wei Yulei , Liu Xinyu , Ge Shengnan , Zhang Haiyang , Che Xinyang , Liu Shiyuan , Liu Debin , Li Huixin , Gu Xinru , He Lin , Li Zuotong , Xu Jingyu
Published: June 2022

Phospholipase C is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phosphoinositide-specific PLC (PI-PLC) and non-specific PLC (NPC), depending on its hydrolytic substrate. In maize, the function of phospholipase C has not been well characterized. In this study, the phospholipase C inhibitor neomycin sulfate (NS, 100 mM) was applied to maize seedlings to investigate the function of maize PLC. Under the treatment of neomycin sulfate, the growth and development of maize seedlings were impaired, and the leaves were gradually etiolated and wilted. The analysis of physiological and biochemical parameters revealed that inhibition of phospholipase C affected photosynthesis, photosynthetic pigment accumulation, carbon metabolism and the stability of the cell membrane. High-throughput RNA-seq was conducted, and differentially expressed genes (DEGS) were found significantly enriched in photosynthesis and carbon metabolism pathways. When phospholipase C activity was inhibited, the expression of genes related to photosynthetic pigment accumulation was decreased, which led to lowered chlorophyll. Most of the genes related to PSI, PSII and TCA cycles were down-regulated and the net photosynthesis was decreased. Meanwhile, genes related to starch and sucrose metabolism, the pentose phosphate pathway and the glycolysis/gluconeogenesis pathway were up-regulated, which explained the reduction of starch and total soluble sugar content in the leaves of maize seedlings. These findings suggest that phospholipase C plays a key role in photosynthesis and the growth and development of maize seedlings.
Telemedicine during COVID-19 Crisis and in Post-Pandemic/Post-Vaccine World—Historical Overview, Current Utilization, and Innovative Practices to Increase Utilization
Authors: Singh Jitendra , Albertson April , Sillerud Brandi
Published: June 2022

Telemedicine’s underutilization ended when the COVID-19 pandemic caused people to isolate and kept them from seeking healthcare services at their local hospitals and clinics. With the aid of the CARES Act of March 2020, healthcare providers quickly implemented telemedicine services to meet the various needs of their patients. During the pandemic, healthcare systems saw a significant increase in telemedicine visits. Essential industries turned to healthcare providers for assistance in keeping their workers healthy and to maintain production in the country’s critical infrastructure. Telemedicine services could quickly address health concerns, help address industry needs, and combat workforce shortages. As quickly as telemedicine services grew, telemedicine service utilization waned as people started to move closer to a pre-pandemic lifestyle. This descriptive study builds on an in-depth literature review by utilizing a fishbone diagram and SWOT analysis examining the potential factors related to telemedicine underutilization. To promote telemedicine utilization, application of Rogers’ Diffusion of Innovation theory outlines how to gain support for the benefits of telemedicine and build on opportunities brought out by the COVID-19 pandemic. Implication for practice could include establishing virtual clinics for industries plagued with workforce shortages.
Behavioral and Emotional Changes One Year after the First Lockdown Induced by COVID-19 in a French Adult Population
Authors: Rousset Sylvie , Level Aurélie , François Florine , Muller Laurent
Published: June 2022

(1) Background: The lockdown had various consequences on physical activity and food consumption behaviors. The post-lockdown has been much less studied. The aim of this study is to compare behaviors one year after the first lockdown in a group of normal-weight (NW) or overweight French adults (OW). (2) Methods: Over a period of 4 days, both at the beginning of May 2020 (lockdown) and in June 2021 (free living post-lockdown), the same French adults used the WellBeNet smartphone application to record their sedentary behavior, physical activity (PA), food consumption and emotions. (3) Results: One year post first lockdown, the weight and body mass index increased (+1.1 kg; +0.4 kg.m−2, p < 0.01), and sedentary behaviors increased (+5.5%, p < 0.01) to the detriment of light-intensity activities (−3.3%, p = 0.10) in the whole group. Some food categories, such as alcohol, tended to be consumed more (+0.15 portion/day, p = 0.09), while fatty, salty and sugary products decreased (−0.25 portion/d, p = 0.02) but without a change in the food balance score. A higher number of both positive and negative emotions were scored per day (+9.5, p < 0.0001; +2.9, p = 0.03), and the positive ones were perceived stronger (+0.23, p = 0.09). Simultaneously, the desire to eat was lower (−11.6/100, p < 0.0001), and the desire to move remained constant. Sedentary/active behaviors and the desire to eat changed differently in NW and OW adults after the lockdown. (4) Conclusions: In general, the post-lockdown period was less favorable for physical activity practice and resulted in a similar food balance score but was more conducive to mental wellbeing.
Impact of a Maternal Motivational Interviewing on Oral Health in the Mother-Child Dyad
Authors: Ramírez-Trujillo M. Á. , Villanueva-Vilchis M. C. , Gaitán-Cepeda L. A. , Aguilar-Díaz F. C. , Rojas-Russell M. E. , Fuente-Hernández J.
Published: June 2022

Motivational Interviewing (MI) has been included in dentistry programs. There exists a need for interventions in the mother-child dyad. The aim of this paper was to compare the effects of a MI-based educational program on oral care knowledge, attitudes, and practices (KAP) in the mother-child dyad to a Traditional Education-based program (TE). A community intervention trial was carried out. The experimental and control groups were made up of women between 18 and 45 years of age in the sixth month of gestation. Both groups were provided with TE. The experimental group additionally received a session based on the principles of the MI. Socio-demographic data, children’s oral health KAP (COHKAP), that of the mother (MOHKAP), and maternal self-efficacy (MSE) in relation to children’s oral health (COHMSE) were recorded. A baseline measurement was made, as well as a six-month follow up. The participants included 135 women with an average age of 24.88 ± 6.00. After intervention, the experimental group’s COHKAP, MOHKAP, and COHMSE all increased (p < 0.001). When MI-based interventions are combined with TE, MSE and dental care KAP for the mother-child dyad in pregnant women can be improved.
Comparative Evaluation of Yield and Fruit Physico-Chemical Characteristics of Five Commercial Cultivars of Pomegranate Grown in Southeastern Italy in Two Consecutive Years
Authors: Tarantino Annalisa , Frabboni Laura , Mazzeo Andrea , Ferrara Giuseppe , Disciglio Grazia
Published: June 2022

This study, conducted over two consecutive seasons (2018–2019), evaluates the yield and fruit physico-chemical traits of five commercial cultivars (‘Mollar’, ‘Dente di Cavallo’, ‘Acco’, ‘Jolly red’ and ‘Wonderful’) grown in the orchard at Foggia (Puglia region, southeastern Italy). The results show significant variations among the investigated cultivars for many measured parameters; seasons also significantly affected some data. As an average of the years, both ‘Dente di Cavallo’ and ‘Wonderful’ significantly showed the highest numbers (16.8 and 15.2, respectively) and weights (9.3 Kg, respectively) of fruit per tree. ‘Jolly red’ had the highest percentage of edible portions (58.5%), the most intense red color in juice (a* value, 11.7), a light-red skin color (despite its name) (a* value, 31.1) and the smallest fruits (26.7% of diameter ≤ 80 mm). Total soluble solids of all cultivars were considerable above the minimum value (12 °Brix) required for commercial use. ‘Wonderful’ significantly presented the highest value of juice acidity (2.5 g citric acid 100 mL−1) and the lowest MI value, and therefore it was classified as sour. Both ‘Dente di Cavallo’ and ‘Wonderful’ had significantly higher phenolic contents (591.0 and 519.1 mg 100 mL−1, respectively) than the other cultivars, while ‘Dente di Cavallo’ significantly showed the highest antioxidant activity value (6.4 mmol TE 100 L−1). The Stepwise Discriminant Analysis procedure allowed a significant differentiation among the cultivars. Correlation analysis showed a considerable relation among the different fruit traits. The present data are helpful in the selection of elite desirable pomegranate cultivars to be used for commercial production.
Limiting the Collection of Ground Truth Data for Land Use and Land Cover Maps with Machine Learning Algorithms
Authors: Ali Usman , Esau Travis J. , Farooque Aitazaz A. , Zaman Qamar U. , Abbas Farhat , Bilodeau Mathieu F.
Published: June 2022

Land use and land cover (LULC) classification maps help understand the state and trends of agricultural production and provide insights for applications in environmental monitoring. One of the major downfalls of the LULC technique is inherently linked to its need for ground truth data to cross-validate maps. This paper aimed at evaluating the efficiency of machine learning (ML) in limiting the use of ground truth data for LULC maps. This was accomplished by (1) extracting reliable LULC information from Sentinel-2 and Landsat-8 s images, (2) generating remote sensing indices used to train ML algorithms, and (3) comparing the results with ground truth data. The remote sensing indices that were tested include the difference vegetation index (DVI), the normalized difference vegetation index (NDVI), the normalized built-up index (NDBI), the urban index (UI), and the normalized bare land index (NBLI). Extracted vegetation indices were evaluated on three ML algorithms, namely, random forest (RF), k-nearest neighbour (K-NN), and k dimensional-tree (KD-Tree). The accuracy of these algorithms was assessed with standard statistical measures and ground truth data randomly collected in Prince Edward Island, Canada. Results showed that high kappa coefficient values were achieved by K-NN (82% and 74%), KD-Tree (80% and 78%), and RF (83% and 73%) for Sentinel-2A and Landsat-8 imagery, respectively. RF was a better classifier than K-NN and KD-Tree and had the highest overall accuracy with Sentinel-2A satellite images (92%). This approach provides the basis for limiting the collection of ground truth data and thus reduces the labour cost, time, and resources needed to collect ground truth data for LULC maps.
Quality Assurance for Spatial Research Data
Authors: Wagner Michael , Henzen Christin
Published: June 2022

In Earth System Sciences (ESS), spatial data are increasingly used for impact research and decision-making. To support the stakeholders’ decision, the quality of the spatial data and its assurance play a major role. We present concepts and a workflow to assure the quality of ESS data. Our concepts and workflow are designed along the research data life cycle and include criteria for openness, FAIRness of data (findable, accessible, interoperable, reusable), data maturity, and data quality. Existing data maturity concepts describe (community-specific) maturity matrices, e.g., for meteorological data. These concepts assign a variety of maturity metrics to discrete levels to facilitate evaluation of the data. Moreover, the use of easy-to-understand level numbers enables quick recognition of highly mature data, and hence fosters easier reusability. Here, we propose a revised maturity matrix for ESS data including a comprehensive list of FAIR criteria. To foster the compatibility with the developed maturity matrix approach, we developed a spatial data quality matrix that relates the data maturity levels to quality metrics. The maturity and quality levels are then assigned to the phases of the data life cycle. With implementing openness criteria and matrices for data maturity and quality, we build a quality assurance (QA) workflow that comprises various activities and roles. To support researchers in applying this workflow, we implement an interactive questionnaire in the tool RDMO (research data management organizer) to collaboratively manage and monitor all QA activities. This can serve as a blueprint for use-case-specific QA for other datasets. As a proof of concept, we successfully applied our criteria for openness, data maturity, and data quality to the publicly available SPAM2010 (crop distribution) dataset series.
Current Methods for Recombination Detection in Bacteria
Authors: Shikov Anton E. , Malovichko Yury V. , Nizhnikov Anton A. , Antonets Kirill S.
Published: June 2022

The role of genetic exchanges, i.e., homologous recombination (HR) and horizontal gene transfer (HGT), in bacteria cannot be overestimated for it is a pivotal mechanism leading to their evolution and adaptation, thus, tracking the signs of recombination and HGT events is importance both for fundamental and applied science. To date, dozens of bioinformatics tools for revealing recombination signals are available, however, their pros and cons as well as the spectra of solvable tasks have not yet been systematically reviewed. Moreover, there are two major groups of software. One aims to infer evidence of HR, while the other only deals with horizontal gene transfer (HGT). However, despite seemingly different goals, all the methods use similar algorithmic approaches, and the processes are interconnected in terms of genomic evolution influencing each other. In this review, we propose a classification of novel instruments for both HR and HGT detection based on the genomic consequences of recombination. In this context, we summarize available methodologies paying particular attention to the type of traceable events for which a certain program has been designed.
Genome-Wide Identification and Analysis of Ariadne Gene Family Reveal Its Genetic Effects on Agronomic Traits of Brassica napus
Authors: Wahid Sumbal , Xie Meili , Sarfraz Sehrish , Liu Jie , Zhao Chuanji , Bai Zetao , Tong Chaobo , Cheng Xiaohui , Gao Feng , Liu Shengyi
Published: June 2022

E3 ligases promote protein ubiquitination and degradation, which regulate every aspect of eukaryotic life. The Ariadne (ARI) proteins of RBR (ring between ring fingers) protein subfamily has been discovered as a group of potential E3 ubiquitin ligases. Only a few available research studies show their role in plant adaptations processes against the external environment. Presently, the functions of ARI proteins are largely unknown in plants. Therefore, in this study, we performed genome-wide analysis to identify the ARI gene family and explore their potential importance in B. napus. A total of 39 ARI genes were identified in the B. napus genome and were classified into three subfamilies (A, B and C) based on phylogenetic analysis. The protein–protein interaction networks and enrichment analysis indicated that BnARI genes could be involved in endoreduplication, DNA repair, proteasome assembly, ubiquitination, protein kinase activity and stress adaptation. The transcriptome data analysis in various tissues provided us an indication of some BnARI genes’ functional importance in tissue development. We also identified potential BnARI genes that were significantly responsive towards the abiotic stresses. Furthermore, eight BnARI genes were identified as candidate genes for multiple agronomic traits through association mapping analysis in B. napus; among them, BnaA02g12100D, which is the ortholog of AtARI8, was significantly associated with ten agronomic traits. This study provided useful information on BnARI genes, which could aid targeted functional research and genetic improvement for breeding in B. napus.
Novel Anti Double-Stranded Nucleic Acids Full-Length Recombinant Camelid Heavy-Chain Antibody for the Detection of miRNA
Authors: Czarnecka Malgorzata , Weichelt Ulrike , Rödiger Stefan , Hanack Katja
Published: June 2022

The discovery that certain diseases have specific miRNA signatures which correspond to disease progression opens a new biomarker category. The detection of these small non-coding RNAs is performed routinely using body fluids or tissues with real-time PCR, next-generation sequencing, or amplification-based miRNA assays. Antibody-based detection systems allow an easy onset handling compared to PCR or sequencing and can be considered as alternative methods to support miRNA diagnostic in the future. In this study, we describe the generation of a camelid heavy-chain-only antibody specifically recognizing miRNAs to establish an antibody-based detection method. The generation of nucleic acid-specific binders is a challenge. We selected camelid binders via phage display, expressed them as VHH as well as full-length antibodies, and characterized the binding to several miRNAs from a signature specific for dilated cardiomyopathy. The described workflow can be used to create miRNA-specific binders and establish antibody-based detection methods to provide an additional way to analyze disease-specific miRNA signatures.
Impact of Doxorubicin on Cell-Substrate Topology
Authors: Krecsir Andreas , Richter Verena , Wagner Michael , Schneckenburger Herbert
Published: June 2022

Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM) is applied in view of early detection of cellular responses to the cytostatic drug doxorubicin. Therefore, we determined cell-substrate topology of cultivated CHO cells transfected with a membrane-associated Green Fluorescent Protein (GFP) in the nanometer range prior to and subsequent to the application of doxorubicin. Cell-substrate distances increased up to a factor of 2 after 24 h of application. A reduction of these distances by again a factor 2 was observed upon cell aging, and an influence of the cultivation time is presently discussed. Applicability of VA-TIRFM was supported by measurements of MCF-7 breast cancer cells after membrane staining and incubation with doxorubicin, when cell-substrate distances increased again by a factor ≥ 2. So far, our method needs well-defined cell ages and staining of cell membranes or transfection with GFP or related molecules. Use of intrinsic fluorescence or even light-scattering methods to various cancer cell lines could make this method more universal in the future, e.g., in the context of early detection of apoptosis.
Parasitism of Corn Earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), by Tachinid Flies in Cultivated Hemp
Authors: Falcon-Brindis Armando , Stireman John O. , Viloria Zenaida J. , Villanueva Raul T.
Published: June 2022

In a survey on hemp grown in western Kentucky we found an average of 27.8 CEW larvae per plant. We recorded 45% parasitism of CEW in these fields by two species of tachinid flies, Winthemia rufopicta and Lespesia aletiae. Most parasitized larvae were third to sixth instars at the time of collection. We found up to 22 tachinid eggs per host larva, 89% of which typically bore between 1 and 5 eggs on the thorax. 45.9% of CEW bearing eggs died. The number of tachinid eggs per host was unrelated to host body mass, but both the number of tachinid eggs and caterpillar body mass influenced CEW survival. Larger CEW often survived parasitism and the number of fly eggs was negatively related to survival rate. The emergence of adult flies was positively correlated with the number of eggs, but no influence of the host size was found. High mortality of CEW larvae and the parasitoids developing within them in this system suggests that secondary chemicals (or poor nutrition) of the hemp diet may be negatively affecting host and parasitoid development and influencing their interactions.
Fertility Preserving Surgery Outcomes for Ovarian Malignancy: Data from a Tertiary Cancer Centre in Central London
Authors: Gaughran Jonathan , Rosen O’Sullivan Hannah , Lyne Tom , Abdelbar Ahmed , Abdalla Mostafa , Sayasneh Ahmad
Published: June 2022

Fertility Sparing Surgery (FSS) appears to be a safe means of treating early-stage ovarian cancer based on relatively limited evidence. However, there is currently insufficient evidence to aid women in counselling about their potential fertility outcomes. The aim of this study was to assess the reproductive outcomes and prognosis of women who have undergone FSS for ovarian malignancy. Between 1 June 2008 and 1 June 2018, a retrospective review of a clinical database was conducted to identify all consecutive patients who underwent FSS in a central London gynaecological oncology centre. All patients with a histological diagnosis of ovarian malignancy (excluding borderline ovarian tumours) were eligible. All identified patients were then prospectively called into a follow up and asked to complete a questionnaire about their fertility outcomes. A total of 47 women underwent FSS; 36 were included in this study. The mean age was 30.3 years (95% Confidence Interval [CI]: 27.6 to 33.0 years). During the study period, 17/36 (47.2%) of the women had attempted to conceive following surgery, with a successful live birth rate of 52.9% (9/17). The mean time of recurrence was 125.3 months (95% CI: 106.5–144.1 months). The mean time to death was 139.5 months (95% CI: 124.3–154.8). The cancer grade, tumour stage and use of Assisted Reproductive Technology (ART) were the main factors significantly associated with the risk of recurrence and death. In conclusion, this study suggests that a large proportion of women will not attempt to conceive following FSS. For those who do attempt to conceive, the likelihood of achieving a live birth is high. However, careful counselling about the higher risk of recurrence and worse survival for women with high grade cancer, disease Stage > IA and potentially those who undergo ART is essential before contemplating FFS.
Durable Mechanical Circulatory Support in Adult Congenital Heart Disease: Reviewing Clinical Considerations and Experience
Authors: Saef Joshua , Montgomery Robert , Cedars Ari , Tang Wai H. Wilson , Rossano Joseph W. , Maeda Katsuhide , Kim Yuli Y. , Vaikunth Sumeet S.
Published: June 2022

Adults with congenital heart disease (ACHD) patients are one of the fastest growing populations in cardiology, and heart failure (HF) is the most common cause of morbidity and mortality amongst them. The need for advanced HF therapies in ACHD patients stands to grow substantially. The anatomic considerations for placing durable mechanical circulatory support (MCS) devices in ACHD patients often require specialized approaches. Despite this, increasing evidence suggests that durable MCS can be implanted safely with favorable outcomes in ACHD patients. Expansion of MCS use in ACHD patients is imperative to improve their clinical outcomes. Knowledge of ACHD-specific anatomic and physiologic considerations is crucial to HF programs’ success as they work to provide care to this growing population.