bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat-killed Salmonella Typhimurium protects mice against carbon ion radiation
Authors: Liao Zebin , Liu Zhe , Gong Zhenyu , Hu Xuguang , Chen Yuanyuan , Cao Kun , Zhang Hong , Gan Lu , Chen Juxiang , Yang Yanyong , Cai Jianming
Published: October 2020

Objective

Patients receiving carbon-ion radiation therapy and astronauts exploring outer space are inevitably exposed to heavy ion radiation. The aim of this study was to develop radioprotectors to minimize the injuries induced by carbon ion radiation.

Methods

Heat-killed Salmonella Typhimurium (HKST) was administered to mice by gavage prior to irradiation with a 12C6+ heavy ion accelerator. Hematoxylin and eosin staining and immunofluorescence TdT-mediated dUTP Nick-End Labeling staining were used to assess the radioprotective effect of HKST on organ damage and levels of apoptosis, respectively, in mice. To investigate the mechanism underlying the radioprotective effect of HKST, levels of the pro-apoptotic proteins BAX and caspase 3 as well as interferon-regulatory factor (IRF) 3/7 in the femur, testis and intestine were assessed using immunofluorescence.

Results

Injuries induced by carbon ion radiation were significantly eased by pretreatment with HKST. Both apoptosis and high expression levels of pro-apoptotic proteins induced by heavy ion radiation were inhibited by HKST pretreatment. The radioprotective effect of HKST was associated with stimulation of Toll-like receptor signaling mediated by enhanced IRF3 and IRF7 signaling.

Conclusion

HKST was an effective radioprotector alleviating damage to multiple organs caused by heavy ion radiation.
Identification of the specific microRNAs and competitive endogenous RNA mechanisms in osteoporosis
Authors: Hong Junyi , Ye Fusheng , Yu Binjia , Gao Junwei , Qi Feicheng , Wang Wei
Published: October 2020

Objective

Osteoporosis and osteoarthritis are metabolic skeletal disorders. This study aimed to identify specific networks of competitive endogenous RNA (ceRNA) in osteoporosis that differ from those in osteoarthritis.

Methods

The dataset GSE74209 was downloaded from the Gene Expression Omnibus, and differentially expressed microRNAs (DEmiRNAs) in osteoporotic samples and osteoarthritic samples were identified. After predicting target genes and linked long noncoding (lnc)RNAs, ceRNA networks of DEmiRNAs were constructed. The nodes that overlapped between ceRNA networks and the Comparative Toxicogenomics Database were selected as key candidates.

Results

Fifteen DEmiRNAs (including 2 downregulated and 13 upregulated miRNAs) were identified in osteoporotic samples versus osteoarthritic samples; these targeted 161 genes and linked to 60 lncRNAs. The ceRNA network consisted of 6 DEmiRNAs, 63 target genes, and 53 lncRNAs. After searching the Comparative Toxicogenomics Database and mining the literature, 2 lncRNAs ( MALAT1 and NEAT1), 2 DEmiRNAs ( hsa- miR- 32-3p, downregulated; and hsa-miR-22-3p, upregulated) and 6 genes ( SP1, PTEN, ESR1, ERBB3, CSF1R, and CDK6) that relate to cell death, growth, and differentiation were identified as key candidates separating osteoporosis from osteoarthritis.

Conclusions

Two miRNA–ceRNA networks (including NEAT1/ MALAT1- hsa- miR- 32- 3p- SP1/ FZD6 and NEAT1/ MALAT1- hsa- miR- 22- 3p- PTEN/ ESR1/ ERBB3/ CSF1R/ CDK6) might have crucial and specific roles in osteoporosis.
Neonatal and Juvenile Ocular Development in Göttingen Minipigs and Domestic Pigs: A Histomorphological and Immunohistochemical Study
Authors: Vrolyk Vanessa , Desmarais Marie-Josée , Lambert Daniel , Haruna Julius , Benoit-Biancamano Marie-Odile
Published: October 2020

Pigs are considered one of the relevant animal models for ocular research as they share several histological and anatomical similarities with the human eye. With the increasing interest in juvenile animal models, this study aimed to describe the postnatal development of ocular structures in 16 Göttingen minipigs and 25 F2 domestic pigs, between birth and 6 months of age, using histopathology and immunohistochemistry against Ki-67, caspase-3, calbindin, glial fibrillary acidic protein, rhodopsin, and synaptophysin. All ocular structures in both pig breeds were incompletely developed at birth and for variable periods postnatally. Noteworthy histological features of immaturity included vascularization in the corneal stroma in neonatal Göttingen minipigs, increased cellularity in different substructures, remnants of the hyaloid vasculature, short and poorly ramified ciliary body processes, and a poorly developed cone inner segment. Increased cellular proliferation, highlighted by abundant Ki-67 immunolabeling, was observed in almost all developing structures of the pig eye for variable periods postnatally. Apoptosis, highlighted with caspase-3 immunolabeling, was observed in the retinal inner nuclear layer at birth and in the regressing hyaloid vasculature remnants. Immunohistochemistry against rhodopsin, synaptophysin, and calbindin demonstrated the short size of the developing photoreceptors and the immature cone inner segment morphology. Calbindin labeling revealed significant differences in the amount of positively labeled cone nuclei between the retinal area centralis and the non–area centralis regions. The elongation of Müller cell processes in the developing retina was shown with glial fibrillary acidic protein. In both pig breeds, the eyes reached histomorphological and immunohistochemical maturity at 6 months of age.
Initial coin offerings: Linking technology and financialization
Authors: Zook Matthew , Grote Michael H.
Published: October 2020

This paper elaborates on the interactions between digital technologies and financial practices and how they contribute to the ongoing process of financialization. We focus on the circumstances of blockchain-based token offerings and their contribution to reshaping existing systems of investment in startups. We show how future clients become investors via the initial coin offering (ICO) process. The paper is based on interviews with blockchain and industry practitioners during 2018 and 2019 and focuses on an in-depth case study of a specific ICO in early 2018. We suggest a framework consisting of catalysts, cracks and voids to analyze the financialization process and to inform theories of how financialization advances through the new spaces afforded by socially constructed technologies upon which entrepreneurs capitalize. With this framework we provide a better understanding of the mechanics behind financialization, particularly the ways in which business processes, and larger social relations such as the role of investors and clients, are reimagined and reworked.
Debris flows originating in the mountain cryosphere under a changing climate: A review
Authors: Yu Guo-An , Yao Weiwei , Huang He Qing , Liu Zhaofei
Published: October 2020

Debris flows originating in the mountain cryosphere (DFMC) are one of the most globally important, widely distributed mass flows (and natural geohazards) in mountain areas with a high altitude and/or high latitude. This is particularly the case in high mountain areas that have been undergoing rapid glacier retreat, permafrost degradation, and other melt/thaw related processes. As a consequence, the actual hazards and potential risks of DFMC have drawn increasing attention in the context of global climate change (i.e. a rising temperature and higher occurrence of strong precipitation events). Unlike debris flows at low elevations, where their occurrence is closely related to precipitation (intensity and duration), the breach of a DFMC event depends on precipitation and/or air temperature, which in turn influence melt/thaw processes, rending the formation mechanism much more complicated. Although research has been widely carried out on DFMC in past decades, there is still a long way to go before we have reached a complete understanding of the formation mechanism and triggering conditions. This review summarizes recent progress in the study of DFMC, including typical DFMC events and their causes, the failure mechanisms of rock (or ice-rock joints), the characteristics of moraine deposits, initiation through hydraulic erosion (entrainment), the relationship between DFMC initiation and meteorological conditions, and the slope stability of the mountain cryosphere under a changing climate. Several issues that should be addressed in future research are also discussed.
Smartphone use and perceptions of their benefit and detriment within Australian anaesthetic practice
Authors: Perkins Emma J , Edelman Daniel A , Brewster David J
Published: October 2020

The primary aim of this study was to evaluate the perceptions of Australian anaesthetists in relation to smartphone use within anaesthetic practice. In particular, we aimed to assess the frequency of smartphone use, the types and number of smartphone applications used, how reliant anaesthetists perceive themselves to be on smartphones and whether they perceive them to be a factor that aids or distracts from their practice. Secondly, we assessed whether there is an association between the type, frequency, reliance and perceptions of smartphone use and the years of experience as an anaesthetist. A 24-item questionnaire addressing these questions was created and distributed to an email list of credentialled anaesthetists in Melbourne, Australia. A total of 113 consultant anaesthetists who practise at 55 hospitals in Melbourne completed the questionnaire. Our results suggest that the majority of anaesthetists are using smartphones regularly in their practice. About 74% of respondents agreed that they rely on their smartphone for their work. We found that respondents were more likely to rely on smartphones and consider them to aid patient safety than to consider them a distraction. This phenomenon was particularly apparent in those who had been a consultant anaesthetist for less than three years. Furthermore, those who had been a consultant anaesthetist for less than three years were more likely to have more smartphone apps relating to anaesthetics, use them more often and rely on them to a greater degree. Our results highlight the ubiquitous and perceived useful nature of smartphones in anaesthetic practice.
Bone marrow-derived mesenchymal stem cells and extracellular vesicles enriched collagen chitosan scaffold in skin wound healing (a rat model)
Authors: Abolgheit Salma , Abdelkader Sally , Aboushelib Moustafa , Omar Enas , Mehanna Radwa
Published: October 2020

Background

Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold’s effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing.

Methods

A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8).

Results

The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group.

Conclusion

The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.
Foreign Aid Effectiveness: Evidence from Panel Data Analysis
Authors: Kabir M. Adnan
Published: October 2020

The effectiveness of foreign aid is an important issue that encompasses a wide range of academic fields but fails to provide any underlying consensus. This study empirically investigates the effectiveness of foreign aid in reducing income inequality of the developing world and subsamples of countries from Africa, South Asia, and South America, which historically demonstrate socioeconomic and geopolitical similarities. In an attempt to recognize aid effectiveness with clarity, this study contributes to the debate in the literature to reconcile the seemingly composite effect of foreign aid on income inequality and extrapolate if the inhibitory mechanisms of institution quality have a regressing effect. Thus, central to the thesis are two intertwining legacies: (a) the possible egalitarian effect of foreign aid on the income distribution of a country and (b) aid effectiveness when a country’s institutional quality is factored in. Using panel data from eight subsamples, the study found statistically significant but marginal foreign aid effectiveness in tackling the income inequality divide of most developing countries. Moreover, the effectiveness diminishes when institutional quality is factored in, which implies a confounding yet deracinating effect on foreign aid efficacy.
What States Can Do to Address Out-of-Network Air Ambulance Bills
Authors: Fuse Brown Erin C. , McDonald Alex , Nguyen Ngan T.
Published: October 2020

Out-of-network air ambulance bills are a pernicious and financially devastating type of surprise medical bill. Courts have broadly interpreted the Airline Deregulation Act to preempt most state attempts to regulate air ambulance billing abuses, so a federal solution is ultimately needed. However, in the absence of a federal fix, states have experimented with a variety of approaches that may survive preemption and provide some protections for their citizens
Filling a Federal Void
Authors: Blake Valarie K. , McGowan Michelle L.
Published: October 2020

Federal law often avoids setting minimum standards for women’s health and reproductive rights issues, leaving legislative and regulatory gaps for the states to fill as they see fit. This has mixed results. It can lead to state innovation that improves state-level health outcomes, informs federal health reform, and provides data on best practices for other states. On the other hand, some states may use the absence of a federal floor to impose draconian policies that pose risks to women’s and maternal health. Health reforms at the federal level must trod carefully to enable state innovation, while imposing foundational safeguards for promoting women’s health nationwide.
Structural Racism and Health Disparities
Authors: Yearby Ruqaiijah
Published: October 2020

The government recognizes that social factors cause racial inequalities in access to resources and opportunities that result in racial health disparities. However, this recognition fails to acknowledge the root cause of these racial inequalities: structural racism. As a result, racial health disparities persist.
Comparison of Population-Representative and Volunteer Experiments: Lessons From the Social Security Administration’s Benefit Offset National Demonstration (BOND)
Authors: Stapleton David C. , Bell Stephen H. , Hoffman Denise , Wood Michelle
Published: October 2020

The Benefit Offset National Demonstration (BOND) tested a $1 reduction in benefits per $2 earnings increase above the level at which Social Security Disability Insurance benefits drop from full to zero under current law. BOND included a rare and large “population-representative” experiment: It applied the rule to a nationwide, random sample of beneficiaries. BOND also included a parallel “volunteer” experiment: The same offset rules applied to a nationwide random sample of volunteers. BOND’s dual experiments offer a unique opportunity to consider the merits and limitations of these two types of experiments. The findings provide a way to illustrate the limitations of volunteer experiments relative to population experiments when, as in BOND, the goal is to produce impact estimates that are valid for the entire population, not just would be volunteers. We also highlight and compare ethical issues, outreach challenges, and cost considerations for the two types of designs.
Thermographic assessment of skin and soft tissue tumors in cats
Authors: Nitrini Andressa Gianotti Campos , Cogliati Bruno , Matera Julia Maria
Published: October 2020

Objectives

This study set out to determine the average temperature of skin and soft tissue tumors in cats using infrared thermography and to investigate correlations between thermographic findings and tumor type. Correlations between thermographic findings, histologic subtype and tumor grade were also investigated in cases of feline injection site sarcoma (FISS).

Methods

Thermographic images of normal skin and skin overlying neoplastic lesions were prospectively obtained. Following thermographic assessment, tumors were resected and submitted to histopathologic and immunohistochemical analysis. Mean temperatures detected in tumoral areas were compared between different tumor types and between FISSs of different histologic subtypes and grades.

Results

Thermograms obtained from 11 healthy cats and 31 cats presenting with skin and soft tissue tumors (eight benign and 23 malignant tumors, including 21 FISSs) were evaluated in this study. Thermal behavior varied widely in normal skin, as well as in skin overlying neoplastic lesions. Mean temperatures were significantly higher in malignant compared with benign tumors (35.4 ± 1.8ºC and 34.5 ± 1.7ºC respectively; P = 0.01), with a temperature above 34.7ºC being associated with malignancy (sensitivity 76%, specificity 80%; P = 0.01). Temperatures detected in FISS did not differ significantly according to histologic subtype ( P = 0.91) or tumor grade ( P = 0.46), or between primary and recurring tumors ( P = 0.25).

Conclusions and relevance

Infrared thermography proved to be a sensitive and effective method for detection of temperature differences between malignant and benign skin and soft tissue tumors in cats. Thermographic assessment may contribute to diagnosis and prognostic estimation in feline oncologic patients.
Drug content on receipt and over time for compounded formulations of famciclovir
Authors: Johnson Lynelle R , Weaver Phoebe G , Forsythe Lauren E , Thomasy Sara M , Knych Heather K
Published: October 2020

Objectives

The aim of this study was to determine famciclovir content (strength) in compounded formulations and to determine if potency changed over time.

Methods

Four concentrations of oral oil suspension in three distinct flavors, three concentrations of oral paste, three chew treats and 62.5 mg tablets from one compounding pharmacy were evaluated for famciclovir content. Specific sample preparation procedures were used for each drug formulation prior to determination of famciclovir content through mass spectrometry tandem liquid chromatography. Analysis was performed on arrival from the compounder and on days 7, 14, 28, 56 and 120. Samples were run in triplicate and concentration determined by comparison with a standard curve. Content was considered appropriate if within 90–110% of the labeled concentration.

Results

On arrival from the compounding pharmacy, 5/12 oral oil suspensions of varying concentrations were <90% of the labeled concentration and 3/3 oral pastes were >110%. Famciclovir content in oil suspensions ranged from 72% to 118% of the label value while oral pastes ranged from 95% to 202% of the label concentration over the 120 study days, and all concentrations varied in an unpredictable fashion. Tablets contained 90–110% of the labeled value throughout the study period. Chew treats could not be successfully analyzed.

Conclusions and relevance

This study found substantial variation in famciclovir content in the compounded products evaluated, which, in turn, raises concerns that substandard dosing could result in lack of efficacy or a failed treatment trial. Drug toxicity might also be encountered. Veterinarians must be aware that while compounded medications can improve compliance, they might not deliver the drug dose expected.
Safe use of Peripherally Inserted Central Catheters for chemotherapy of solid malignancies in adult patients: A 1-year monocentric, prospectively-assessed, unselected cohort of 482 patients
Authors: Piredda Alessio , Radice Davide , Zencovich Claudia , Cerri Martina , Aventino Lucia , Naccarato Francesco , Magon Giorgio , Biffi Roberto
Published: October 2020

Introduction:

Aim of this study was to analyze the overall complication and failure rates of Peripherally Inserted Central Catheters (PICCs), in a 1-year consecutive unselected cohort of 482 adult patients, affected by non-hematological malignancies undergoing chemotherapy.

Methods:

Adult outpatients (aged 18–75 years), with an Eastern Cooperative Oncology Group (ECOG) performance status of 0–2, bearing solid tumors and candidates for intravenous chemotherapy were eligible for the study. Exclusion criteria were active infections, coagulopathy (defined as platelet count <50,000/μL and/or prothrombin time more than 18 s), life expectancy <6 months, or inability to give written informed consent. Devices were all implanted in an outpatients’ hospital facility, following predefined evidence-based institutional guidelines and protocols by a PICC-dedicated team at the European Institute of Oncology in Milan, Italy, during the 12-month period from January 1 to December 31, 2019.

Results:

Five-hundred PICCs were implanted in a cohort of 482 patients during the time interval of this study. Thirty devices were overall removed (6.2%), 23 as a consequence of a complication occurred, and seven inadvertently. The inserted PICCs accounted for a total of 49,718 catheter days in situ, median duration was 85.5 days [interquartile range (IQR): 56–146]. Overall there were 42 (8.7%) complications, corresponding to 0.84 catheter-adverse events (CAE)/1000 PICC-days (95% CI: 0.61–1.14). There were N = 13 (2.7%) thromboses, N = 11 (2.3%) irreversible occlusions, N = 7 (1.5%) accidental removals, N = 5 (1.0%) infections [two Catheter Related Blood Stream Infection (CRBSI) and three exit site/local infection], N = 3 (0.6%) ruptures and N = 3 (0.6%) primary or secondary malpositions.

Conclusion:

This large prospective study supports the increasing use of PICCs in adult oncology outpatients treated in specialized centers with chemotherapy for non-hematological malignancies. In this clinical setting, PICC failure occurred in 6% only of the inserted devices.
Finding the bird’s wings: Dimensions of factional conflict on Twitter
Authors: Sältzer Marius
Published: October 2020

Intra-party politics has long been neglected due to lacking data sources. While we have a good understanding of the dynamics of ideological competition between parties, we know less about how individuals or groups inside parties influence policy, leadership selection and coalition bargaining. These questions can only be answered if we can place individual politicians and sub-party groups like factions on the same dimensions as in inter-party competition. This task has been notoriously difficult, as most existing measures either work on the party level, or are in other ways determined by the party agenda. Social media is a new data source that allows analyzing positions of individual politicians in party-centered systems, as it is subject to limited party control. I apply canonical correspondence analysis to account for hierarchical data structures and estimate multidimensional positions of the Twitter accounts of 498 Members of the German Bundestag based on more than 800,000 tweets since 2017. To test the effect of intra-party actors on their relative ideological placement, I coded the faction membership of 247 Twitter users in the Bundestag. I show that Twitter text reproduces party positions and dimensions. Members of factions are more likely to represent their faction’s positions, both on the cultural and the economic dimension.
Heidegger and parenthood: A theoretical and methodological shift from instrumental to ontological understanding
Authors: Darbyshire Philip , Oerther Sarah
Published: October 2020

Parenthood and parenting are concepts central for child and family health nurses and professionals. They are foundational to numerous nursing philosophies such as ‘family-centred care’ and ‘parent participation’. Yet our understanding of the meaning of being a parent remains difficult to articulate and is often operationalised as collections of assessable techniques and skills. We propose an alternative understanding of parenthood, based on the work of Martin Heidegger and his turn to poetry, that is more ontologically focused on the meaning of being a parent and valuable to nurses seeking to understand or research the existential core of this complex relationship. Alternative ways of understanding parenthood will help nurses grasp the complexities of family relationships they will encounter in practice. Researchers may also frame their investigations and explorations of parenting and parent–child–professional relationships in ways that do not rely exclusively on ‘technologies’ of parenting skills and techniques. Heidegger’s thinking opens up valuable ways of exploring, understanding and researching parenthood that can benefit nurses in clinical practice, education and research. In its ability to challenge the most fundamental of assumptions and to propose challenging alternatives, Heideggerian approaches to understanding the meaning of parenthood can help advance child and family nursing research and practice.
Explaining Uncontested Seats in Congress and State Legislatures
Authors: Burden Barry C. , Snyder Rochelle
Published: October 2020

A fundamental requirement of democracy is the existence of contested elections. Our study documents and explains trends in uncontested seats in the U.S. Congress and state legislatures over time. We uncover a striking inconsistency in the health of elections: the frequency of uncontested seats in Congress has declined while the frequency of uncontested seats in state legislatures has actually increased. To explore these divergent trends, we consider factors that are common to both Congress and state legislatures such as the redistricting cycle but also variables that are unique to the state level. Our analysis points to the relative “flippability” of Congress compared to many state legislatures as a factor behind diverging levels of contestation. While many state legislatures have become bastions for dominant parties, congressional districts in those same states are often nonetheless viewed as enticing targets because they contribute to control of the federal government.
Localization and function of a Plasmodium falciparum protein (PF3D7_1459400) during erythrocyte invasion
Authors: Amlabu Emmanuel , Nyarko Prince B , Opoku Grace , Ibrahim-Dey Damata , Ilani Philip , Mensah-Brown Henrietta , Akporh Grace A , Akuh Ojo-Ajogu , Ayugane Evelyn A , Amoh-Boateng David , Kusi Kwadwo A , Awandare Gordon A
Published: October 2020

Nearly 60% of Plasmodium falciparum proteins are still uncharacterized and their functions are unknown. In this report, we carried out the functional characterization of a 45 kDa protein (PF3D7_1459400) and showed its potential as a target for blood stage malaria vaccine development. Analysis of protein subcellular localization, native protein expression profile, and erythrocyte invasion inhibition of both clinical and laboratory parasite strains by peptide antibodies suggest a functional role of PF3D7_1459400 protein during erythrocyte invasion. Also, immunoreactivity screens using synthetic peptides of the protein showed that adults resident in malaria endemic regions in Ghana have naturally acquired plasma antibodies against PF3D7_1459400 protein. Altogether, this study presents PF3D7_1459400 protein as a potential target for the development of peptide-based vaccine for blood-stage malaria.

Impact statement

Plasmodium falciparum malaria is a global health problem. Erythrocyte invasion by P. falciparum merozoites appears to be a promising target to curb malaria. We have identified and characterized a novel protein that is involved in erythrocyte invasion. Our data on protein subcellular localization, stage-specific protein expression pattern, and merozoite invasion inhibition by α-peptide antibodies suggest a role for PF3D7_1459400 protein during P. falciparum erythrocyte invasion. Even more, the human immunoepidemiology data present PF3D7_1459400 protein as an immunogenic antigen which could be further exploited for the development of new anti-infective therapy against malaria.
Exploring influenza vaccine hesitancy in community pharmacies: Knowledge, attitudes and practices of community pharmacists in Ontario, Canada
Authors: Pullagura Gokul Raj , Violette Richard , Houle Sherilyn K. D. , Waite Nancy M.
Published: October 2020

Background:

Vaccine hesitancy (VH) remains a prime contributor to poor influenza vaccine uptake. This study explores the knowledge, attitudes and practices of community pharmacists toward influenza VH, including their personal influenza immunization attitudes and behaviours.

Methods:

A web-based cross-sectional survey questionnaire was administered to community pharmacists practising in Ontario, Canada. A 38-question survey tool explored 5 domains, including pharmacists’ personal attitudes and behaviour toward influenza immunization, their self-reported knowledge of influenza, its vaccine and vaccine hesitancy, and their attitudes, practices and experiences with influenza VH at the community pharmacy. The data were analyzed descriptively.

Results:

A total of 5530 survey invitations were e-mailed, and 885 responses were collected (response rate 16%). Two-thirds ( n = 568, 65.7%) of the respondents reported receiving the influenza vaccine in the preceding season. The most frequent reasons for personal influenza immunization were prevention of disease transmission to patients, friends and family, and contribution to herd immunity. In addition to their confidence and perceived ability to identify and address influenza VH, respondents’ self-reported knowledge across a 15-item Likert questionnaire was high. Respondents reported coming across an average of 16 (SD 28) individuals hesitant to receive the influenza vaccine each week. Regular workload ( n = 419, 65.6%) and insufficient time ( n = 406, 65.3%) were reported as the most limiting barriers to engagement in influenza vaccine conversations.

Conclusion:

Facilitating optimal practice scope for pharmacists, and capitalizing on the convenience and accessibility of the community pharmacy setting, presents a promising means to address influenza VH. However, barriers to pharmacist-initiated engagement on influenza vaccine must be explored and addressed. Can Pharm J (Ott) 2020;153:xx-xx.
Syrian refugees in digital news discourse: Depictions and reflections in Germany
Authors: Mustafa-Awad Zahra , Kirner-Ludwig Monika
Published: October 2020

This study examines the topical frames reflected in articles published about Syrian refugees by German, British and American news websites in 2016. We analyze these for terms associated with Syrian refugees and the themes they address then relate them to those we identified for 2015 and to those indicated by German students in expressing their attitudes towards them. The results show that, despite discrepancies in the occurrence of Syrian refugees’ collocates in our 2016 news corpora, they still reflect, at a lower rate, the themes identified for 2015: humanitarianism, conflict, dehumanization, security, economic burden, political responsibility, and integration. The survey results confirm humanitarianism and integration as particularly prominent themes for our participants, too. While our findings provide further discursive evidence for the variation in refugees’ representations in news discourse and for the influence of media on public opinion, they suggest that this impact may be balanced out by other factors including an audience’s critical reading and thinking skills.
Clinical considerations about the coexistence of melanoma and chronic lymphocytic leukemia in the era of targeted therapies, triggered by rare clinical scenarios. A case series and review of the literature
Authors: Diamantopoulos Panagiotis T. , Ziogas Dimitrios , Viniou Nora-Athina , Anastasopoulou Amalia , Kyriakakis Georgios , Frangia Konstantina , Gogas Helen
Published: October 2020

The epidemiologic correlation of melanoma and chronic lymphocytic leukemia (CLL) has been the subject of several population studies. In the present article, through the presentation of five illustrative cases of patients with melanoma and CLL, several aspects of this complex relationship are highlighted, with a focus on the increased incidence of melanoma in patients with CLL, its speculated etiology, and the impact of CLL stage and disease duration on the incidence and prognosis of melanoma. Furthermore, the rare entity of the synchronous diagnosis of melanoma and CLL in biopsied lymph nodes is discussed, along with its implications on the diagnostic and therapeutic procedures. In addition, the available data on the treatment choices in patients with melanoma and CLL are presented and the efficacy and safety of fludarabine, anti-CD20 monoclonal antibodies, new targeted therapies for CLL, and checkpoint inhibitors are further discussed. Finally, since no formal guidelines are available for the management of this group of patients, guidelines are proposed for skin-cancer screening in patients with CLL, for the correct interpretation of BRAF mutation analysis in lymph-node specimens with ‘collision of tumors,’ and for the optimal use of imaging studies in the diagnosis of metastatic disease in patients with CLL and melanoma, while a treatment approach for such patients is also suggested. The information and proposed guidelines provided in the present article comprise a useful guide for physicians managing such patients, focusing on diagnostic challenges and therapeutic dilemmas posed by the coexistence of the two disease entities.
Patient Experience of Obstetric Care During the COVID-19 Pandemic: Preliminary Results From a Recurring National Survey
Authors: Bradley Dani , Blaine Arianna , Shah Neel , Mehrotra Ateev , Gupta Rahul , Wolfberg Adam
Published: October 2020

The experience of pregnant and postpartum patients continues to evolve during the COVID-19 pandemic. Limited clinical data and the unknown nature of the virus’ impact and transmission routes have forced constant changes to traditional care delivery. Dependence on telehealth technology such as telephonic and videoconferencing has surged, and patients’ willingness to visit traditional health care facilities has plummeted. We set out to create an ongoing surveillance system to monitor changes to prenatal and obstetric care and the patient experience during the COVID-19 pandemic.
Pharmacy Students Perceptions of Their Distance Online Learning Experience During the COVID-19 Pandemic: A Cross-Sectional Survey Study
Authors: Shawaqfeh Mohammad S , Al Bekairy Abdulkareem M , Al-Azayzih Ahmad , Alkatheri Abdulmalik A , Qandil Amjad M , Obaidat Aiman A , Al Harbi Shemylan , Muflih Suhaib M
Published: October 2020

Introduction:

The COVID-19 pandemic imposed dramatic changes on educational practices worldwide. Many universities and schools have moved into the delivery of their courses and educational programs utilizing fully electronic online modes. This study aims to evaluate the pharmacy student distance online learning experience during the COVID-19 pandemic.

Methods:

A cross-sectional survey was utilized where a 3-domain survey questionnaire focused on preparedness, attitude and barriers was distributed to students at the time of conclusion of the semester. Each domain consists of multiple questions that made up a score that reflects their preparedness, attitude as well as barriers relevant to distance online learning experience. The survey was voluntarily, and all data were collected and recorded via google forms with maintaining anonymity.

Results:

The response rate was about 75% (n = 309). The results’ analysis revealed no gender differences in any of these domains. However, there were some variable responses among different educational levels. The average preparedness score was 32.8 ± 7.2 (Max 45), the average attitude score was 66.8 ± 16.6 (Max 105), and the average barrier score was 43.6 ± 12.0 (Max 75). There was statistical significance difference in both preparedness score and attitude scores between different professional years ( P-value <.05). However, there was no difference in barrier scores among all professional years. The results indicated that about 61.4% of the students agreed on that college of pharmacy was well-prepared and ready for the online education during the emerging COVID-19 pandemic with complete transition into online education. The results also indicated that 49.2% of the students showed positive attitude toward the provided online learning. The results indicated that about 34% of the students identify some barriers toward the provided online learning. Finally, there were strong association between the need for training on how to receive online courses and preparedness and barriers scores.

Discussion and conclusion:

E-learning experience pose challenges and presents opportunities during emergency situations. The need for training for students and faculty was highly associated with the preparedness and barriers domains rather than the infrastructure or computer literacy, so the school can improve their experience by addressing these needs.
Roundtable Discussion on COVID-19 Through a Sex and Gender Lens
Authors: Legato Marianne J. , Bennett Wendy L. , Klein Sabra , Sheffield Jeanne S. , Morgan Rosemary , Decker Michele R. , Sharps Phyllis
Published: October 2020

Although the full and lasting impact of the coronavirus disease 2019 (COVID-19) outbreak is yet to be determined, there is evidence that sex and gender play a significant role in determining patient outcomes across the globe. This roundtable discussion is a transcript of a seminar held by several representatives from Johns Hopkins University on the impact of the global pandemic on women’s health and well-being. They reported on the various pathophysiological aspects of the disease, as well as the social and financial consequences of this global pandemic. Looking at COVID-19 through a sex and gender lens highlights the vulnerabilities and inequalities of people of different genders, races, and socioeconomic conditions, and how care providers can better respond to those differences.
The Transplant Evaluation Rating Scale predicts overall survival after allogeneic hematopoietic stem cell transplantation
Authors: Solh Melhem M. , Speckhart Dawn , Solomon Scott R. , Bashey Asad , Morris Lawrence E. , Zhang Xu , Holland H. Kent
Published: October 2020

To evaluate the impact of psychosocial risks on post–hematopoietic stem cell transplantation (HSCT) outcomes, we prospectively conducted psychosocial assessment of 556 consecutive allogeneic HSCT patients who received their first allogeneic transplant at our center between 2003 and 2017. The Transplant Evaluation Rating Scale (TERS) score was prospectively assessed by a psychologist before transplantation, and patients were categorized as low, intermediate, or high risk based on their TERS score. Patients in the high-risk TERS group had significantly longer hospital stays during the first 180 days and 1 year post–allogeneic HSCT compared with the low-risk group (16 vs 13 and 21 vs 16 days; P = .05 and .02, respectively). The survival estimates for low-, intermediate-, and high-risk TERS groups at 3 year were as follows: overall survival (OS), 73%, 60%, and 65%; disease-free survival (DFS), 63%, 55%, and 60%; nonrelapse mortality (NRM), 11%, 20%, and 17%; and relapse, 26%, 25%, and 23%, respectively. In a multivariable analysis, intermediate- and high-risk TERS scores predicted for inferior OS, similar DFS, and higher NRM compared with low-risk TERS score. In a subset analysis of patients with low/intermediate risk per Disease Risk Index, multivariable analysis showed that high- and intermediate-risk TERS scores predicted for significantly worse OS, worse DFS, higher NRM, and similar relapse rates compared with low-risk TERS score. Our findings show that psychosocial factors as measured by TERS score are strong predictors of morbidity and mortality after HSCT among patients with low/intermediate disease risk.
Cells in Silico – introducing a high-performance framework for large-scale tissue modeling
Authors: Berghoff Marco , Rosenbauer Jakob , Hoffmann Felix , Schug Alexander
Published: October 2020

Background

Discoveries in cellular dynamics and tissue development constantly reshape our understanding of fundamental biological processes such as embryogenesis, wound-healing, and tumorigenesis. High-quality microscopy data and ever-improving understanding of single-cell effects rapidly accelerate new discoveries. Still, many computational models either describe few cells highly detailed or larger cell ensembles and tissues more coarsely. Here, we connect these two scales in a joint theoretical model.

Results

We developed a highly parallel version of the cellular Potts model that can be flexibly applied and provides an agent-based model driving cellular events. The model can be modular extended to a multi-model simulation on both scales. Based on the NAStJA framework, a scaling implementation running efficiently on high-performance computing systems was realized. We demonstrate independence of bias in our approach as well as excellent scaling behavior.

Conclusions

Our model scales approximately linear beyond 10,000 cores and thus enables the simulation of large-scale three-dimensional tissues only confined by available computational resources. The strict modular design allows arbitrary models to be configured flexibly and enables applications in a wide range of research questions. Cells in Silico (CiS) can be easily molded to different model assumptions and help push computational scientists to expand their simulations to a new area in tissue simulations. As an example we highlight a 10003 voxel-sized cancerous tissue simulation at sub-cellular resolution.
Construction of an 11-microRNA-based signature and a prognostic nomogram to predict the overall survival of head and neck squamous cell carcinoma patients
Authors: Huang Yusheng , Liu Zhiguo , Zhong Limei , Wen Yi , Ye Qixiang , Cao Donglin , Li Peiwu , Liu Yufeng
Published: October 2020

Background

Head and neck squamous cell carcinoma (HNSCC) is a fatal malignancy owing to the lack of effective tools to predict overall survival (OS). MicroRNAs (miRNAs) play an important role in HNSCC occurrence, development, invasion and metastasis, significantly affecting the OS of patients. Thus, the construction of miRNA-based risk signatures and nomograms is desirable to predict the OS of patients with HNSCC. Accordingly, in the present study, miRNA sequencing data of 71 HNSCC and 13 normal samples downloaded from The Cancer Genome Atlas (TCGA) were screened to identify differentially expressed miRNAs (DEMs) between HNSCC patients and normal controls. Based on the exclusion criteria, the clinical information and miRNA sequencing data of 67 HNSCC samples were selected and used to establish a miRNA-based signature and a prognostic nomogram. Forty-three HNSCC samples were assigned to an internal validation cohort for verifying the credibility and accuracy of the primary cohort. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore the functions of 11 miRNA target genes.

Results

In total, 11 DEMs were successfully identified. An 11-miRNA risk signature and a prognostic nomogram were constructed based on the expression levels of these 11 DEMs and clinical information. The signature and nomogram were further validated by calculating the C-index, area under the curve (AUC) in receiver-operating characteristic curve analysis, and calibration curves, which revealed their promising performance. The results of the internal validation cohort shown the reliable predictive accuracy both of the miRNA-based signature and the prognostic nomogram. GO and KEGG analyses revealed that a mass of signal pathways participated in HNSCC proliferation and metastasis.

Conclusion

Overall, we constructed an 11-miRNA-based signature and a prognostic nomogram with excellent accuracy for predicting the OS of patients with HNSCC.
Vibrionaceae core, shell and cloud genes are non-randomly distributed on Chr 1: An hypothesis that links the genomic location of genes with their intracellular placement
Authors: Sonnenberg Cecilie Bækkedal , Kahlke Tim , Haugen Peik
Published: October 2020

Background

The genome of Vibrionaceae bacteria, which consists of two circular chromosomes, is replicated in a highly ordered fashion. In fast-growing bacteria, multifork replication results in higher gene copy numbers and increased expression of genes located close to the origin of replication of Chr 1 (ori1). This is believed to be a growth optimization strategy to satisfy the high demand of essential growth factors during fast growth. The relationship between ori1-proximate growth-related genes and gene expression during fast growth has been investigated by many researchers. However, it remains unclear which other gene categories that are present close to ori1 and if expression of all ori1-proximate genes is increased during fast growth, or if expression is selectively elevated for certain gene categories.

Results

We calculated the pangenome of all complete genomes from the Vibrionaceae family and mapped the four pangene categories, core, softcore, shell and cloud, to their chromosomal positions. This revealed that core and softcore genes were found heavily biased towards ori1, while shell genes were overrepresented at the opposite part of Chr 1 (i.e., close to ter1). RNA-seq of Aliivibrio salmonicida and Vibrio natriegens showed global gene expression patterns that consistently correlated with chromosomal distance to ori1. Despite a biased gene distribution pattern, all pangene categories contributed to a skewed expression pattern at fast-growing conditions, whereas at slow-growing conditions, softcore, shell and cloud genes were responsible for elevated expression.

Conclusion

The pangene categories were non-randomly organized on Chr 1, with an overrepresentation of core and softcore genes around ori1, and overrepresentation of shell and cloud genes around ter1. Furthermore, we mapped our gene distribution data on to the intracellular positioning of chromatin described for V. cholerae, and found that core/softcore and shell/cloud genes appear enriched at two spatially separated intracellular regions. Based on these observations, we hypothesize that there is a link between the genomic location of genes and their cellular placement.