bluuebunny/crossref_metadata_2025 huggingface dataset:
Innate Immune Response of TmToll-3 Following Systemic Microbial Infection in Tenebrio molitor
Authors: Ali Mohammadie Kojour Maryam , Jang Ho Am , Lee Yong Seok , Jo Yong Hun , Han Yeon Soo
Published: April 2023

Although Toll-like receptors have been widely identified and functionally characterized in mammalian models and Drosophila, the immunological function of these receptors in other insects remains unclear. Here, we explored the relevant innate immune response of Tenebrio molitor (T. molitor) Toll-3 against Gram-negative bacteria, Gram-positive bacteria, and fungal infections. Our findings indicated that TmToll-3 expression was mainly induced by Candida albicans infections in the fat bodies, gut, Malpighian tubules, and hemolymph of young T. molitor larvae. Surprisingly, Escherichia coli systemic infection caused mortality after TmToll-3 knockdown via RNA interference (RNAi) injection, which was not observed in the control group. Further analyses indicated that in the absence of TmToll-3, the final effector of the Toll signaling pathway, antimicrobial peptide (AMP) genes and relevant transcription factors were significantly downregulated after E. coli challenge. Our results indicated that the expression of almost all AMP genes was suppressed in silenced individuals, whereas the expression of relevant genes was positively regulated after fungal injection. Therefore, this study revealed the immunological involvement of TmToll-3 in T. molitor in response to systematic infections.
NAGS, CPS1, and SLC25A13 (Citrin) at the Crossroads of Arginine and Pyrimidines Metabolism in Tumor Cells
Authors: Owusu-Ansah Melissa , Guptan Nikita , Alindogan Dylon , Morizono Michio , Caldovic Ljubica
Published: April 2023

Urea cycle enzymes and transporters collectively convert ammonia into urea in the liver. Aberrant overexpression of carbamylphosphate synthetase 1 (CPS1) and SLC25A13 (citrin) genes has been associated with faster proliferation of tumor cells due to metabolic reprogramming that increases the activity of the CAD complex and pyrimidine biosynthesis. N-acetylglutamate (NAG), produced by NAG synthase (NAGS), is an essential activator of CPS1. Although NAGS is expressed in lung cancer derived cell lines, expression of the NAGS gene and its product was not evaluated in tumors with aberrant expression of CPS1 and citrin. We used data mining approaches to identify tumor types that exhibit aberrant overexpression of NAGS, CPS1, and citrin genes, and evaluated factors that may contribute to increased expression of the three genes and their products in tumors. Median expression of NAGS, CPS1, and citrin mRNA was higher in glioblastoma multiforme (GBM), glioma, and stomach adenocarcinoma (STAD) samples compared to the matched normal tissue. Median expression of CPS1 and citrin mRNA was higher in the lung adenocarcinoma (LUAD) sample while expression of NAGS mRNA did not differ. High NAGS expression was associated with an unfavorable outcome in patients with glioblastoma and GBM. Low NAGS expression was associated with an unfavorable outcome in patients with LUAD. Patterns of DNase hypersensitive sites and histone modifications in the upstream regulatory regions of NAGS, CPS1, and citrin genes were similar in liver tissue, lung tissue, and A549 lung adenocarcinoma cells despite different expression levels of the three genes in the liver and lung. Citrin gene copy numbers correlated with its mRNA expression in glioblastoma, GBM, LUAD, and STAD samples. There was little overlap between NAGS, CPS1, and citrin sequence variants found in patients with respective deficiencies, tumor samples, and individuals without known rare genetic diseases. The correlation between NAGS, CPS1, and citrin mRNA expression in the individual glioblastoma, GBM, LUAD, and STAD samples was very weak. These results suggest that the increased cytoplasmic supply of either carbamylphosphate, produced by CPS1, or aspartate may be sufficient to promote tumorigenesis, as well as the need for an alternative explanation of CPS1 activity in the absence of NAGS expression and NAG.
Antibiotic Resistance Genes in Aerosols: Baseline from Kuwait
Authors: Habibi Nazima , Uddin Saif , Behbehani Montaha , Kishk Mohamed , Abdul Razzack Nasreem , Zakir Farhana , Shajan Anisha
Published: April 2023

Antimicrobial resistance (AMR) is one of the biggest threats to human health worldwide. The World Health Organization (WHO, Geneva, Switzerland) has launched the “One-Health” approach, which encourages assessment of antibiotic-resistant genes (ARGs) within environments shared by human-animals-plants-microbes to constrain and alleviate the development of AMR. Aerosols as a medium to disseminate ARGs, have received minimal attention. In the present study, we investigated the distribution and abundance of ARGs in indoor and outdoor aerosols collected from an urban location in Kuwait and the interior of three hospitals. The high throughput quantitative polymerase chain reaction (HT-qPCR) approach was used for this purpose. The results demonstrate the presence of aminoglycoside, beta-lactam, fluoroquinolone, tetracycline, macrolide-lincosamide-streptogramin B (MLSB), multidrug-resistant (MDR) and vancomycin-resistant genes in the aerosols. The most dominant drug class was beta-lactam and the genes were IMP-2-group (0.85), Per-2 group (0.65), OXA-54 (0.57), QnrS (0.50) and OXA-55 (0.55) in the urban non-clinical settings. The indoor aerosols possessed a richer diversity (Observed, Chao1, Shannon’s and Pielou’s evenness) of ARGs compared to the outdoors. Seasonal variations (autumn vs. winter) in relative abundances and types of ARGs were also recorded (R2 of 0.132 at p < 0.08). The presence of ARGs was found in both the inhalable (2.1 µm, 1.1 µm, 0.7 µm and < 0.3 µm) and respirable (>9.0 µm, 5.8 µm, 4.7 µm and 3.3 µm) size fractions within hospital aerosols. All the ARGs are of pathogenic bacterial origin and are hosted by pathogenic forms. The findings present baseline data and underpin the need for detailed investigations looking at aerosol as a vehicle for ARG dissemination among human and non-human terrestrial biota.
The Impact of BDNF, NTRK2, NGFR, CREB1, GSK3B, AKT, MAPK1, MTOR, PTEN, ARC, and SYN1 Genetic Polymorphisms in Antidepressant Treatment Response Phenotypes
Authors: Santos Marlene , Lima Luis , Carvalho Serafim , Mota-Pereira Jorge , Pimentel Paulo , Maia Dulce , Correia Diana , Barroso M. Fátima , Gomes Sofia , Cruz Agostinho , Medeiros Rui
Published: April 2023

This study aimed to investigate the influence of genetic variants in neuroplasticity-related genes on antidepressant treatment phenotypes. The BDNF-TrkB signaling pathway, as well as the downstream kinases Akt and ERK and the mTOR pathway, have been implicated in depression and neuroplasticity. However, clinicians still struggle with the unpredictability of antidepressant responses in depressed patients. We genotyped 26 polymorphisms in BDNF, NTRK2, NGFR, CREB1, GSK3B, AKT, MAPK1, MTOR, PTEN, ARC, and SYN1 in 80 patients with major depressive disorder treated according to the Texas Medical Algorithm for 27 months at Hospital Magalhães Lemos, Porto, Portugal. Our results showed that BDNF rs6265, PTEN rs12569998, and SYN1 rs1142636 SNP were associated with treatment-resistant depression (TRD). Additionally, MAPK1 rs6928 and GSK3B rs6438552 gene polymorphisms were associated with relapse. Moreover, we found a link between the rs6928 MAPK1 polymorphism and time to relapse. These findings suggest that the BDNF, PTEN, and SYN1 genes may play a role in the development of TRD, while MAPK1 and GSK3B may be associated with relapse. GO analysis revealed enrichment in synaptic and trans-synaptic transmission pathways and glutamate receptor activity with TRD-associated genes. Genetic variants in these genes could potentially be incorporated into predictive models of antidepressant response.
Intracellular Zinc Trafficking during Crotalus atrox Venom Wound Development
Authors: Albrecht Eric A. , Carter Jasmine D. , Garbar Veronica , Choudhary Abeeha , Tomlins Scott A.
Published: April 2023

In this study, we examined zinc trafficking in human umbilical vein endothelial cells (HUVEC) stimulated with Crotalus atrox (CA venom) snake venom. We utilized MTS cytotoxicity assays to monitor the cytotoxic range of CA venom. HUVEC monolayers stimulated with 10 µg/mL CA venom for 3 h displayed cellular retraction, which coincided with 53.0 ± 6.5 percent viability. In contrast, venom concentrations of 100 µg/mL produced a complete disruption of cellular adherence and viability decreased to 36.6 ± 1.0. The zinc probe Fluozin-3AM was used to detect intracellular zinc in non-stimulated controls, HUVEC stimulated with 10 µg/mL CA venom or HUVEC preincubated with TPEN for 2 h then stimulated with 10 µg/mL CA venom. Fluorescent intensity analysis returned values of 1434.3 ± 197.4 for CA venom demonstrating an increase of about two orders of magnitude in labile zinc compared to non-stimulated controls. Endothelial response to CA venom induced a 96.1 ± 3.0- and 4.4 ± 0.41-fold increase in metallothionein 1X (MT1X) and metallothionein 2A (MT2A) gene expression. Zinc chelation during CA venom stimulation significantly increased cell viability, suggesting that the maintenance of zinc homeostasis during envenomation injury improves cell survival.
First In Vivo Insights on the Effects of Tempol-Methoxycinnamate, a New UV Filter, as Alternative to Octyl Methoxycinnamate, on Zebrafish Early Development
Authors: Damiani Elisabetta , Sella Fiorenza , Astolfi Paola , Galeazzi Roberta , Carnevali Oliana , Maradonna Francesca
Published: April 2023

The demand for organic UV filters as active components in sunscreen products has rapidly risen over the last century, as people have gradually realized the hazards of overexposure to UV radiation. Their extensive usage has resulted in their ubiquitous presence in different aquatic matrices, representing a potential threat to living organisms. In this context, the need to replace classic UV filters such as octyl methoxycinnamate (OMC), one of the most popular UV filters reported to be a potential pollutant of aquatic ecosystems, with more environmentally friendly ones has emerged. In this study, using zebrafish, the first in vivo results regarding the effect of exposure to tempol-methoxycinnamate (TMC), a derivative of OMC, are reported. A comparative study between TMC and OMC was performed, analyzing embryos exposed to similar TMC and OMC concentrations, focusing on morphological and molecular changes. While both compounds seemed not to affect hatching and embryogenesis, OMC exposure caused an increase in endoplasmic reticulum (ER) stress response genes, according to increased eif2ak3, ddit3, nrf2, and nkap mRNA levels and in oxidative stress genes, as observed from modulation of the sod1, sod2, gpr, and trx mRNA levels. On the contrary, exposure to TMC led to reduced toxicity, probably due to the presence of the nitroxide group in the compound’s molecular structure responsible for antioxidant activity. In addition, both UV filters were docked with estrogen and androgen receptors where they acted differently, in agreement with the molecular analysis that showed a hormone-like activity for OMC but not for TMC. Overall, the results indicate the suitability of TMC as an alternative, environmentally safer UV filter.
Hepatic, Muscle and Intestinal Oxidative Status and Plasmatic Parameters of Greater Amberjack (Seriola dumerili, Risso, 1810) Fed Diets with Fish Oil Replacement and Probiotic Addition
Authors: Milián-Sorribes Maria Consolación , Peres Helena , Tomás-Vidal Ana , Moutinho Sara , Peñaranda David S. , Jover-Cerdá Miguel , Oliva-Teles Aires , Martínez-Llorens Silvia
Published: April 2023

The present study was conducted to investigate the effects of dietary fish oil replacement with a mixture of vegetable oils and probiotic supplementation on plasma biochemical parameters, oxidative stress, and antioxidant ability of Seriola dumerili. Specimens with an initial weight of 175 g were used. Four feeds were formulated with 0% (FO-100), 75% (FO-25), and 100% (FO-0 and FO-0+ with the addition of Lactobacillus probiotics) substitution of fish oil with a mixture of linseed, sunflower, and palm oils. After 109 days, no significant differences were observed in the activity of antioxidant enzymes in the liver, foregut, and hindgut, only glucose-6-phosphate dehydrogenase activity in the liver was higher in the fish fed the FO-100 diet than in those fed the FO-0 diet. No significant differences were observed in the total, reduced, and oxidized glutathione and the oxidative stress index in the liver. In addition, lipid peroxidation in the liver and red muscle values were higher in the fish fed the FO-100 diet than in the fish fed the FO-0+ diet, however, the foregut of the fish fed the FO-100 diet presented lower values than that of the fish fed the FO replacement diet, with and without probiotics. There were significant differences in cholesterol levels in the FO-100 group; they were significantly higher than those observed with the fish diets without fish oil. To sum up, fish oil can be replaced by up to 25% with vegetable oils in diets for Seriola dumerili juveniles, but total fish oil substitution is not feasible because it causes poor survival. The inclusion of probiotics in the FO-0+ diet had no effects on the parameters measured.
Integrated QTL Mapping, Meta-Analysis, and RNA-Sequencing Reveal Candidate Genes for Maize Deep-Sowing Tolerance
Authors: Zhao Xiaoqiang , Niu Yining , Hossain Zakir , Shi Jing , Mao Taotao , Bai Xiaodong
Published: April 2023

Synergetic elongation of mesocotyl and coleoptile are crucial in governing maize seedlings emergence, especially for the maize sown in deep soil. Studying the genomic regions controlling maize deep-sowing tolerance would aid the development of new varieties that are resistant to harsh conditions, such as drought and low temperature during seed germination. Using 346 F2:3 maize population families from W64A × K12 cross at three sowing depths, we identified 33 quantitative trait loci (QTLs) for the emergence rate, mesocotyl, coleoptile, and seedling lengths via composite interval mapping (CIM). These loci explained 2.89% to 14.17% of phenotypic variation in a single environment, while 12 of 13 major QTLs were identified at two or more sowing environments. Among those, four major QTLs in Bin 1.09, Bin 4.08, Bin 6.01, and Bin 7.02 supported pleiotropy for multiple deep-sowing tolerant traits. Meta-analysis identified 17 meta-QTLs (MQTLs) based on 130 original QTLs from present and previous studies. RNA-Sequencing of mesocotyl and coleoptile in both parents (W64A and K12) at 3 cm and 20 cm sowing environments identified 50 candidate genes expressed differentially in all major QTLs and MQTLs regions: six involved in the circadian clock, 27 associated with phytohormones biosynthesis and signal transduction, seven controlled lignin biosynthesis, five regulated cell wall organization formation and stabilization, three were responsible for sucrose and starch metabolism, and two in the antioxidant enzyme system. These genes with highly interconnected networks may form a complex molecular mechanism of maize deep-sowing tolerance. Findings of this study will facilitate the construction of molecular modules for deep-sowing tolerance in maize. The major QTLs and MQTLs identified could be used in marker-assisted breeding to develop elite maize varieties.
A Dual Stream Generative Adversarial Network with Phase Awareness for Speech Enhancement
Authors: Liang Xintao , Li Yuhang , Li Xiaomin , Zhang Yue , Ding Youdong
Published: April 2023

Implementing single-channel speech enhancement under unknown noise conditions is a challenging problem. Most existing time-frequency domain methods are based on the amplitude spectrogram, and these methods often ignore the phase mismatch between noisy speech and clean speech, which largely limits the performance of speech enhancement. To solve the phase mismatch problem and further improve enhancement performance, this paper proposes a dual-stream Generative Adversarial Network (GAN) with phase awareness, named DPGAN. Our generator uses a dual-stream structure to predict amplitude and phase separately and adds an information communication module between the two streams to fully apply the phase information. To make the prediction more efficient, we apply Transformer to build the generator, which can learn the sound’s structural properties more easily. Finally, we designed a perceptually guided discriminator that quantitatively evaluates the quality of speech, optimising the generator for specific evaluation metrics. We conducted experiments on the most widely used Voicebank-DEMAND dataset and DPGAN achieved state-of-the-art on most metrics.
Prediction of Strain in Embedded Rebars for RC Member, Application of Hybrid Learning Approach
Authors: Mirzazade Ali , Popescu Cosmin , Täljsten Björn
Published: April 2023

The aim of this study was to find strains in embedded reinforcement by monitoring surface deformations. Compared with analytical methods, application of the machine learning regression technique imparts a noteworthy reduction in modeling complexity caused by the tension stiffening effect. The present research aimed to achieve a hybrid learning approach for non-contact prediction of embedded strains based on surface deformations monitored by digital image correlation (DIC). However, due to the small training dataset collected by the installed strain gauges, the input dataset was enriched by a semi-empirical equation proposed in a previous study. Therefore, the present study discussed (i) instrumentation by strain gauge and DIC as well as data acquisition and post-processing of the data, accounting for strain gradients on the concrete surface and embedded reinforcement; (ii) input dataset generation for training machine learning regression models approaching hybrid learning; (iii) data regression to predict strains in embedded reinforcement based on monitored surface deformations; and (iv) the results, validation, and post-processing responses to make the method more robust. Finally, the developed model was evaluated through numerous statistical performance measures. The results showed that the proposed method can reasonably predict strain in embedded reinforcement, providing an innovative type of sensing application with highly improved performance.
Harnessing the Power of Precision Medicine and Novel Biomarkers to Treat Crohn’s Disease
Authors: Kriger-Sharabi Ofra , Kopylov Uri
Published: April 2023

Crohn’s disease (CD) is a chronic inflammatory condition that affects the gastrointestinal tract. It is part of a spectrum of inflammatory Bowel Diseases (IBD). The disease is complex, characterized by significant inter and intra-individual heterogeneity, which contributes to a diverse and multifaceted portrayal of the disease. Consequently, applying specific and accurate treatment is challenging, and therapeutic success rates remain disappointing and insufficient. In recent years, significant advances in the therapeutic potential of CD have been made. Hope has been provided by these developments in the form of an expanding treatment toolkit. However, even with these beneficial adjustments, patients are frequently treated using an ineffective “one size fits all” treatment protocol, ultimately leading to a plateau in drug effectiveness and a decline in overall treatment success rates. Furthermore, with the advancement in the genome-wide association study, in combination with significant bioinformatic developments, the world of medicine has moved in the direction of personalized, tailored-treatment medicine, and this trend has not escaped the world of IBDs. Prediction models, novel biomarkers, and complex algorithms are emerging and inspiring optimism that CD patients will be treated with “precision medicine” in the near future, meaning that their treatments will be selected based on the patient’s various unique features. In this review, we will outline the current diagnostic and therapeutic limitations that lead to a glass ceiling effect and thus send us in pursuit of discovering novel biomarkers. We will illustrate the challenges and difficulties in discovering relevant and innovative biomarkers and implementing them into everyday clinical practice. We will also heighten the progress made in practicing personalized medicine for CD patients and shed light on future directions and horizons.
Current Trends in Volume and Surgical Outcomes in Gastric Cancer
Authors: Marano Luigi , Verre Luigi , Carbone Ludovico , Poto Gianmario Edoardo , Fusario Daniele , Venezia Dario Francesco , Calomino Natale , Kaźmierczak-Siedlecka Karolina , Polom Karol , Marrelli Daniele , Roviello Franco , Kok Johnn Henry Herrera , Vashist Yogesh
Published: April 2023

Gastric cancer is ranked as the fifth most frequently diagnosed type of cancer. Complete resection with adequate lymphadenectomy represents the goal of treatment with curative intent. Quality assurance is a crucial factor in the evaluation of oncological surgical care, and centralization of healthcare in referral hospitals has been proposed in several countries. However, an international agreement about the setting of “high-volume hospitals” as well as “minimum volume standards” has not yet been clearly established. Despite the clear postoperative mortality benefits that have been described for gastric cancer surgery conducted by high-volume surgeons in high-volume hospitals, many authors have highlighted the limitations of a non-composite variable to define the ideal postoperative period. The textbook outcome represents a multidimensional measure assessing the quality of care for cancer patients. Transparent and easily available hospital data will increase patients’ awareness, providing suitable elements for a more informed hospital choice.
Thyroid Metastasis from Primary Breast Cancer
Authors: Patrizio Armando , Ferrari Silvia Martina , Stoppini Giulio , Palmisano Elena , Elia Giusy , Ragusa Francesca , Paparo Sabrina Rosaria , Balestri Eugenia , Mazzi Valeria , Botrini Chiara , Proietti Agnese , Famà Fausto , Benvenga Salvatore , Antonelli Alessandro , Fallahi Poupak
Published: April 2023

Breast cancer (BC), the most commonly diagnosed malignancy, frequently metastasizes to the bone, lungs, brain and liver at advanced stages, whereas the thyroid gland represents a rare target site for secondary disease. We examined the most recent literature about thyroid metastasis (TM) from BC after we encountered a peculiar case of a 71-year-old woman who developed sudden dysphagia, severe hypothyroidism and hypoparathyroidism due to TM 18 years after the diagnosis of her primary cancer. Based on published data, the prevalence of TM in BC ranges from 3% to 34%, with a median onset time of 48.2 months, although longer time intervals are not infrequent. TM negatively impacts the prognosis of these patients, however thyroid surgery can limit the local disease burden. Therefore, we suggest that clinicians involved in the follow-up care of BC patients should consider a differential diagnosis of secondary thyroid malignancy when incidental lesions are diagnosed during radiological evaluations or local symptoms affect the cervical region, even many years after the diagnosis of the primary cancer.
Are We on the Right Track for HCV Micro-Elimination? HCV Management Practices in Dialysis Centers in Poland—A National Cross-Sectional Survey
Authors: Czarnecka Paulina , Czarnecka Kinga , Tronina Olga , Baczkowska Teresa , Zarychta-Wisniewska Weronika , Durlik Magdalena
Published: April 2023

Chronic hepatitis C (CHC) is prevalent in the hemodialysis-dependent population. Currently, all patients with CHC should be considered for treatment; however, many hemodialysis-dependent patients are still left untreated. Following HCV cure, accurate surveillance is mandatory to reduce liver-related mortality and prevent reinfection. We aimed to establish HCV management practices and barriers to HCV elimination in dialysis centers in Poland. Polish dialysis centers were surveyed via email. The HCV management strategies were investigated. Representatives of 112 dialysis centers responded, representing 43.1% of all dialysis centers in Poland and 43.4% of hemodialysis-dependent patients’ volume. Most respondents were Heads of hemodialysis centers and board-certified nephrologists. The study demonstrated that in the vast majority of hemodialysis centers (91.6%), subjects are considered for antiviral treatment (AVT); however, many obstacles preventing patients from being prescribed AVT were identified; patients’ reluctance to undergo AVT was most reported (60%). The majority of dialysis units neither evaluate patients with CHC for liver fibrosis (60.4%) nor screen them for hepatocellular carcinoma (53.5%). In conclusion, the presented study demonstrates that HCV management practices across Polish dialysis centers vary substantially. There is a need to optimize and streamline the HCV management infrastructure in the hemodialysis population in Poland.
EKOSTM in Octogenarians: The Safety and Efficacy of Ultrasound-Accelerated Catheter-Directed Thrombolysis in Elderly Patients with Intermediate-High-Risk Pulmonary Embolism
Authors: Al-Terki Hani , Elhakim Abdelrahman , Mügge Andreas
Published: April 2023

Background: Pulmonary embolism (PE) is a common cardiovascular disease. Elderly patients with acute PE have very high mortality rates. Data concerning the safety and effectiveness of ultrasound-accelerated thrombolysis (USAT) in this age group are lacking. Methods: Nineteen octogenarians with acute pulmonary embolism underwent USAT between August 2020 and February 2023 at two centres in Germany and were retrospectively analysed. The main efficacy measures were the right ventricle to left ventricle diameter (RV/LV) ratio, systolic right ventricle function, and invasive and echocardiographic measured systolic pulmonary artery pressure (sPAP). The main safety measures were in-hospital death and the bleeding rate according to the GUSTO bleeding score. Results: USAT was associated with an improved RV/LV ratio (0.36 ± 0.29, p < 0.001), systolic right ventricle function (5.0 ± 3.8, p < 0.001), and systolic pulmonary artery pressure (sPAP) at 24 h after therapy (24.2 ± 11.2 mmHg and 19 ± 13.4 mmHg, p < 0.001). No in-hospital deaths or bleeding complications occurred. Conclusions: USAT with EKOSTM may be a safe and effective therapeutic option for octogenarians with acute pulmonary embolism.
Calcium Phosphate-Loaded Novel Polypropylene Glycol-Based Dental Resin Composites: Evaluation of In Vitro Bioactivity
Authors: Shafqat Zahra , Munir Nadia , Inayat Naveed , Khan Muhammad Adnan , Fareed Muhammad Amber , Zafar Muhammad Sohail
Published: April 2023

Objective: This study aimed to assess in vitro bioactivity of novel remineralizing dental composites loaded with calcium phosphate fillers and chlorhexidine in polypropylene glycol (PPG) resin matrix. Methods: The stock monomer was prepared by adding 69.75% urethane dimethacrylate and 23.25% of the polypropylene glycol dimethacrylate with silica fillers, chlorhexidine (5 wt%), and varying levels of calcium phosphate fillers. The study groups were BC (basic composite), commercial control, CHX-CP5, CHX-CP10, and CHX-CP15, respectively. Bioactivity was assessed by placing samples in the simulated body fluid (SBF) for 7, 14, and 28 days and observed under the scanning electron microscope and energy dispersive X-ray spectroscopy. Data were presented in mean and percentage with a 95% confidence interval. Intergroup analysis was performed using one-way ANOVA and the p-value was set ≤0.05. Results: The SEM images showed the deposition of calcium phosphate on the surface of CHX-CP10 and CHX-CP15 after 28 days in SBF. Mineral deposits of calcium and phosphate were observed on the surface of the experimental formulation containing higher calcium phosphate fillers (CP10 and CP15) in EDX. Conclusion: The addition of calcium phosphate fillers to the composites resulted in an apatite layer formed and demonstrated enhanced bioactivity in the presence of PPGDMA and CHX.
A Contrastive-Learning-Based Method for the Few-Shot Identification of Ship-Radiated Noises
Authors: Nie Leixin , Li Chao , Wang Haibin , Wang Jun , Zhang Yonglin , Yin Fan , Marzani Franck , Bozorg Grayeli Alexis
Published: April 2023

For identifying each vessel from ship-radiated noises with only a very limited number of data samples available, an approach based on the contrastive learning was proposed. The input was sample pairs in the training, and the parameters of the models were optimized by maximizing the similarity of sample pairs from the same vessel and minimizing that from different vessels. In practical inference, the method calculated the distance between the features of testing samples and those of registration templates and assigned the testing sample into the closest templates for it to achieve the parameter-free classification. Experimental results on different sea-trial data demonstrated the advantages of the proposed method. On the five-ship identification task based on the open-source data, the proposed method achieved an accuracy of 0.68 when only five samples per vessel were available, that was significantly higher than conventional solutions with accuracies of 0.26 and 0.48. Furthermore, the convergence of the method and the behavior of its performance with increasing data samples available for the training were discussed empirically.
Landscape in Spatial Planning: Some Evidence on Methodological Issues and Political Challenges
Authors: Marson Anna
Published: April 2023

In recent decades, the landscape has given a new impulse to the renewal of spatial planning. This process has nevertheless raised several methodological issues about how to deal with sensitive non-functional aspects in spatial planning tools and procedures, as well as new challenges for policy design. Placemaking, landscape urbanism, and landscape planning do not differ just in scale but in their very idea of public/collective interest and the action that is required to reach them. Reflecting on some evidence from the recent Italian experience of landscape plans and policies, based on direct involvement in practice and academic debate, the author will highlight several main issues at stake today in this field. The conclusions will argue some potentially promising innovation perspectives, on both processes and contents regarding landscape-based spatial planning and policies, as well as some critical conditions of an institutional context.
Using a Geotrail for Teaching Geography: An Example of the Virtual Educational Trail “The Story of Liberec Granite”
Authors: Drápela Emil
Published: April 2023

Geotrails are valuable resources for the field teaching of geography and natural history, as they amusingly explain the phenomena associated with inanimate nature and geoheritage. However, not all geotrails are frequently used for field education, in fact, most of them are only used sporadically. In order to identify the key elements needed for a geotrail to be used frequently and successfully for field education, an evaluation of the virtual nature trail “The Story of Liberec Granite”, which is one of the most used trails for educational purposes in the Czech Republic, was carried out. The evaluation was conducted using a questionnaire survey and structured interviews with teachers and other educational staff who have used the geotrail at least three times in their teaching. The results of the evaluation showed that the proximity of the geotrail to the school building is very important, that all geotrail materials must be developed with the target group in mind, i.e., school-age children, and that they must be professionally designed to appeal to the pupils. The geotrail should be accompanied by materials that can be used in field education (such as worksheets), containing tasks for pupils so that they do not remain only the passive recipients of information but become active participants in discerning information. Teachers will also appreciate it if there are methodological materials accompanying the geotrail that explain how to design the fieldwork.
Do the High-Tech Industrial Development Zones Foster Urban Innovation? A Case Study of China
Authors: Wang Qixuan , Ren Jie , Yan Simin , Cao Zhan , Cheng Yao
Published: April 2023

China’s high-tech industrial development zones (HIDZs) is a key “place-based” policy targeting national innovation-driven development. Despite the extensive research on HIDZs, it remains unclear whether and to what extent the policy has fostered cities’ innovation output and quality. Basing our research on panel data of Chinese cities from 2001 to 2019, we employed the PSM-DID model to examine the impact of HIDZs policy on their host cities’ innovation output and quality. The empirical results show that: (1) In general, the establishment of HIDZs has a more significant positive effect on fostering urban innovation output compared to its role in promoting urban innovation quality. However, (2) the effect on urban innovation output and quality varies across different cities. For cities with more advantageous locations and policies, HIDZ policy plays a more instrumental role in promoting the quality of urban innovation, while the establishment of HIDZs in other cities is more conducive to increasing the output of urban innovation. Ultimately, we argue that authorities must recognize the importance of integrated development of HIDZs and their host cities and incorporating HIDZs’ impact on the host cities into the HIDZ evaluation. It is necessary to understand that HIDZs have multiple development modes due to their specific local conditions. Hence, differentiated guidance must be carried out rather than directly replicating the experience from developed regions.
Molecular Mechanism of Sirtuin 1 Inhibition by Human Immunodeficiency Virus 1 Tat Protein
Authors: Adolph Ramona S. , Beck Eileen , Schweimer Kristian , Di Fonzo Andrea , Weyand Michael , Rösch Paul , Wöhrl Birgitta M. , Steegborn Clemens
Published: April 2023

Sirtuins are NAD+-dependent protein lysine deacylases implicated in metabolic regulation and aging-related dysfunctions. The nuclear isoform Sirt1 deacetylates histones and transcription factors and contributes, e.g., to brain and immune cell functions. Upon infection by human immunodeficiency virus 1 (HIV1), Sirt1 deacetylates the viral transactivator of transcription (Tat) protein to promote the expression of the viral genome. Tat, in turn, inhibits Sirt1, leading to the T cell hyperactivation associated with HIV infection. Here, we describe the molecular mechanism of Tat-dependent sirtuin inhibition. Using Tat-derived peptides and recombinant Tat protein, we mapped the inhibitory activity to Tat residues 34–59, comprising Tat core and basic regions and including the Sirt1 deacetylation site Lys50. Tat binds to the sirtuin catalytic core and inhibits Sirt1, Sirt2, and Sirt3 with comparable potencies. Biochemical data and crystal structures of sirtuin complexes with Tat peptides reveal that Tat exploits its intrinsically extended basic region for binding to the sirtuin substrate binding cleft through substrate-like β-strand interactions, supported by charge complementarity. Tat Lys50 is positioned in the sirtuin substrate lysine pocket, although binding and inhibition do not require prior acetylation and rely on subtle differences to the binding of regular substrates. Our results provide mechanistic insights into sirtuin regulation by Tat, improving our understanding of physiological sirtuin regulation and the role of this interaction during HIV1 infection.
Design Optimization of Additive Manufactured Edgeless Simple Cubic Lattice Structures under Compression
Authors: Park Kwang-Min , Roh Young-Sook
Published: April 2023

This study proposed an optimization framework and methodologies to design edgeless lattice structures featuring fillet and multipipe functions. Conventional lattice structures typically experience stress concentration at the sharp edges of strut joints, resulting in reduced mechanical performance and premature failure. The proposed approach aimed to improve the compression behavior of lattice structures by introducing edgeless features. Through finite element analysis, the optimized fillet edgeless simple cubic unit cell with a fillet radius to strut radius ratio of 0.753 showed a 12.1% improvement in yield stress and a 144% reduction in stress concentration. To validate the finite element analysis, experimental compressive tests were conducted, confirming that the introduction of edgeless functions improved the compressive strength of lattice structures manufactured through additive manufacturing. The optimized fillet edgeless simple cubic lattice structure exhibited the most effective improvement. This approach has promising potential for lattice structure applications.
Advances in Regenerated Asphalt Mixtures
Authors: Li Yuanyuan , Bai Tao , Zhang Jizhe , Gao Yangming
Published: April 2023

This Special Issue is devoted to research on asphalt pavement materials, including asphalt binders, asphalt mixtures and recycled asphalt pavement (RAP) [...]
Experimental and Theoretical Studies on Hysteretic Behavior of Friction Energy Dissipation Composite Chord under Quasi-Static Tests
Authors: Zhou Qishi , He Wenwu , Zhou Zhibin , Guo Wenxuan , Liu Shuaishuai
Published: April 2023

To improve the seismic performance of a staggered truss steel framing system, the basic force unit in the truss system is replaced by a friction energy dissipation truss. The difference between a friction energy dissipation truss and an ordinary truss is that the upper chord is a friction energy dissipation composite chord. In this paper, we investigate the effects of the number of bolts and the friction surface on the energy dissipation capacity of the chord by a quasi-static test on six composite chord specimens at a scale of 1:2. The results show that the hysteresis curves of friction energy dissipation composite chords are ideal rectangles, and the energy dissipation capacity is excellent. The more bolts there are in the specimen, the slower the energy dissipation capacity of the chord decreases. Among the different friction surface specimens, the energy dissipation capacity of the aluminum friction plate specimen decays the fastest, while the energy dissipation capacity of the shot-blasted treated specimen decays substantially after the first cycle. Friction plates can improve the stability of the hysteresis properties. Based on the test results, this paper proposes a calculation method for the ultimate bearing capacity of the composite chord, which provides a basis for the design of a friction energy dissipation truss. In addition, we studied the effects of different bolt clamping forces and slotted bolt hole lengths on the energy dissipation capacity of composite chords by establishing a finite element analysis. It was shown that as the clamping force of the bolt increases, the energy dissipation capacity of the specimen becomes stronger but the stability decreases. The energy dissipation capacity of the chord is close to a linear relationship with the slotted bolt hole lengths; thus, increasing the slotted bolt hole lengths within the allowable range of inter-story drifts can enhance the energy dissipation capacity of the chord. Finally, we propose the design method of the angle steel by analyzing the force of the chord.
State of the Art in Wearable Wrist Exoskeletons Part I: Background Needs and Design Requirements
Authors: Pitzalis Roberto Francesco , Park Daegeun , Caldwell Darwin G. , Berselli Giovanni , Ortiz Jesús
Published: April 2023

Despite an increase in the use of exoskeletons, particularly for medical and occupational applications, few studies have focused on the wrist, even though it is the fourth most common site of musculoskeletal pain in the upper limb. The first part of this paper will present the key challenges to be addressed to implement wrist exoskeletons as wearable devices for novel rehabilitation practices and tools in the occupational/industrial sector. Since the wrist is one of the most complex joints in the body, an understanding of the bio-mechanics and musculo-skeletal disorders of the wrist is essential to extracting design requirements. Depending on the application, each wrist exoskeleton has certain specific design requirements. These requirements have been categorized into six sections: purpose, kinematics, dynamics, rigidity, ergonomics, and safety. These form the driving factors behind the choice of a design depending on the objectives. Different design architectures are explored, forming the basis for the various technical challenges that relate to: actuation type, power source, power transmission, sensing, and control architecture. This paper summarizes, in a systematic approach, all the current technologies adopted, analyzes their benefits and limitations, and finally proposes future perspectives.
Density of Some Special Sequences Modulo 1
Authors: Dubickas Artūras
Published: April 2023

In this paper, we explicitly describe all the elements of the sequence of fractional parts {af(n)/n}, n=1,2,3,…, where f(x)∈Z[x] is a nonconstant polynomial with positive leading coefficient and a≥2 is an integer. We also show that each value w={af(n)/n}, where n≥nf and nf is the least positive integer such that f(n)≥n/2 for every n≥nf, is attained by infinitely many terms of this sequence. These results combined with some earlier estimates on the gaps between two elements of a subgroup of the multiplicative group Zm* of the residue ring Zm imply that this sequence is everywhere dense in [0,1]. In the case when f(x)=x this was first established by Cilleruelo et al. by a different method. More generally, we show that the sequence {af(n)/nd}, n=1,2,3,…, is everywhere dense in [0,1] if f∈Z[x] is a nonconstant polynomial with positive leading coefficient and a≥2, d≥1 are integers such that d has no prime divisors other than those of a. In particular, this implies that for any integers a≥2 and b≥1 the sequence of fractional parts {an/nb}, n=1,2,3,…, is everywhere dense in [0,1].
A Study of a Two Storage Single Product Inventory System with Ramp Type Demand, N-Phase Prepayment and Purchase for Exigency
Authors: Viswanath Jagadeesan , Thilagavathi Rajamanickam , Karthik Krishnasamy , Mahdal Miroslav
Published: April 2023

This model considers a two-warehouse inventory system of deteriorated items with ramp-type demand and a constant rate of deterioration. It is maintained a rental warehouse (RW) of infinite capacity to load the excess items of replenished goods after filling the items of finite capacity in the own warehouse (OW). Retailers are encouraged to opt for the prepayment option of paying their purchase cost in equal installments prior to the delivery of the ordered items with a considerable discount, which will ensure the purchase guarantee of their orders. The slotted backlog interval of the stock out period is handled in two different ways to retain the customers and ease their impatience. Customers in the first slot of the stock out period are satisfied by the emergency purchases from local suppliers with high purchasing costs to avoid losing customers. Customers in the next slot are satisfied immediately after the next replenishment point. Essential measures of the system are derived: optimal ordering quantities from both regular and local suppliers; replenishment cycle length; and a partitioned backlog interval. A numerical example is given along with the optimal solutions for a particular environment with sensitive analysis in order to validate the model’s efficacy.
Research on Robust Audio-Visual Speech Recognition Algorithms
Authors: Yang Wenfeng , Li Pengyi , Yang Wei , Liu Yuxing , He Yulong , Petrosian Ovanes , Davydenko Aleksandr
Published: April 2023

Automatic speech recognition (ASR) that relies on audio input suffers from significant degradation in noisy conditions and is particularly vulnerable to speech interference. However, video recordings of speech capture both visual and audio signals, providing a potent source of information for training speech models. Audiovisual speech recognition (AVSR) systems enhance the robustness of ASR by incorporating visual information from lip movements and associated sound production in addition to the auditory input. There are many audiovisual speech recognition models and systems for speech transcription, but most of them have been tested based in a single experimental setting and with a limited dataset. However, a good model should be applicable to any scenario. Our main contributions are: (i) Reproducing the three best-performing audiovisual speech recognition models in the current AVSR research area using the most famous audiovisual databases, LSR2 (Lip Reading Sentences 2) LSR3 (Lip Reading Sentences 3), and comparing and analyzing their performances under various noise conditions. (ii) Based on our experimental and research experiences, we analyzed the problems currently encountered in the AVSR domain, which are summarized as the feature-extraction problem and the domain-generalization problem. (iii) According to the experimental results, the Moco (momentum contrast) + word2vec (word to vector) model has the best AVSR effect on the LRS datasets regardless of whether there is noise or not. Additionally, the model also produced the best experimental results in the experiments of audio recognition and video recognition. Our research lays the foundation for further improving the performance of AVSR models.
An In-Phase Unit Slot-Opening Shift Method for Cogging Torque Reduction in Interior Permanent Magnet Machine
Authors: Wang Linwei , Lu Shuai , Chen Yangming , Wang Shiya
Published: April 2023

The cogging torque reduction methods in permanent magnet (PM) machines are more or less FEA trial and error-based and cause undesirable side effects, such as distorted back EMF, new harmonic components, and structure asymmetry. A slot-opening grouping method can address these issues. However, it needs to model all slots in FEA design validation during practical cogging torque optimization iterations, and also leads to back-EMF reduction. In this paper, a new grouping strategy, termed in-phase unit (IPU), is introduced, together with the slot-opening angle-shift method. The slot openings with the same cogging torque distribution pattern are grouped into an IPU, and the cogging torque peaks of the slot openings within an IPU can now be interleaved. Thereby, the major harmonic components of the cogging torque are cancelled out, instead of being summed up. The IPU grouping principle, the slot-opening shift angle computation, and the selection of the harmonic order to cancel are analyzed in detail. By comparison, the proposed method not only simplifies cogging torque optimization iterations by only modeling the slots in one IPU in FEA, but also compensates for the back-EMF constant reduction. The effectiveness of the proposed methods is validated by two typical interior PM machine design cases.