bluuebunny/crossref_metadata_2025 huggingface dataset:
Lactobacillus plantarum and Lactobacillus reuteri as Functional Feed Additives to Prevent Diarrhoea in Weaned Piglets
Authors: Dell’Anno Matteo , Callegari Maria Luisa , Reggi Serena , Caprarulo Valentina , Giromini Carlotta , Spalletta Ambra , Coranelli Simona , Sgoifo Rossi Carlo Angelo , Rossi Luciana
Published: June 2021

The effects of Lactobacillus plantarum and Lactobacillus reuteri and their combination were assessed in weaned piglets. Three hundred and fifty weaned piglets (Landrace × Large White), balanced in terms of weight and sex, were randomly allotted to four experimental groups (25 pens, 14 piglets/pen). Piglets were fed a basal control diet (CTRL, six pens) and a treatment diet supplemented with 2 × 108 CFU/g of L. plantarum (PLA, 6 pens), 2 × 108 CFU/g L. reuteri (REU, six pens) and the combination of both bacterial strains (1 × 108 CFU/g of L. plantarum combined with 1 × 108 CFU/g of L. reuteri, P+R, 7 pens) for 28 days. Body weight and feed intake were recorded weekly. Diarrhoea occurrence was assessed weekly by the faecal score (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets per pen for microbiological analyses and serum samples were collected from two piglets per pen for serum metabolic profiling. Treatments significantly reduced diarrhoea occurrence and decreased the average faecal score (0.94 ± 0.08 CTRL, 0.31 ± 0.08 PLA, 0.45 ± 0.08 REU, 0.27 ± 0.08 P+R; p < 0.05). The PLA group registered the lowest number of diarrhoea cases compared to other groups (20 cases CTRL, 5 cases PLA, 8 cases REU, 10 cases P+R; p < 0.01). After 28 days, the globulin serum level increased in PLA compared to the other groups (24.91 ± 1.09 g/L CTRL, 28.89 ± 1.03 g/L PLA, 25.91 ± 1.03 g/L REU, 25.31 ± 1.03 g/L P+R; p < 0.05). L. plantarum and L. reuteri could thus be considered as interesting functional additives to prevent diarrhoea occurrence in weaned piglets.
Endemic Cyprus Scops Owl Otus cyprius Readily Breeds in Artificial Nest Boxes
Authors: Iezekiel Savvas , Yosef Reuven , Themistokleus Constantinos , Bakaloudis Dimitrios E. , Vlachos Christos G. , Antoniou Andreas , Iezekiel Eandas , Papakosta Malamati A. , Kosicki Jakub Z.
Published: June 2021

As is well-known, endemic island bird species are especially vulnerable to extinction from anthropogenic environmental change and reduced fitness compared with mainland taxa. The Cyprus Scops Owl, Otus cyprius, is a recently recognized island endemic species whose ecology and breeding biology have not been studied. It nests mainly in holes in trees and buildings, so the felling of old trees, modern architectural practices, and the renovation of old houses in villages may reduce nest site availability. Its population trend is also unknown. Therefore, to better determine its ecological requirements and habitat preferences we placed nest boxes in rural areas adjacent to the forest, in the forest, and in the ecotone between them, and used breeding success as our indicator of habitat suitability. We found that breeding parameters like laying date, clutch size, length of the incubation period, hatching day, hatching success, and number of nestlings did not differ between the three habitats. Despite the low level of nest box occupancy rate (5–11%) the endemic Cyprus Scops Owl readily breeds in artificial nests. Therefore, although we are unaware of any current threats to the Cyprus Scops Owl, we recommend that its conservation be prioritized, including studies, monitoring, habitat conservation, and the provision of nest boxes.
Bacteriophages with Potential to Inactivate Aeromonas hydrophila in Cockles: In Vitro and In Vivo Preliminary Studies
Authors: Duarte João , Pereira Carla , Costa Pedro , Almeida Adelaide
Published: June 2021

The recurrent emergence of infection outbreaks associated with shellfish consumption is of extreme importance for public health. The present study investigated the potential application of phages AH-1, AH-4, and AH-5 to inactivate Aeromonas hydrophila, a causative agent of infections in humans associated with bivalve shellfish consumption. The inactivation of A. hydrophila was assessed in vitro, using a liquid culture medium, and in vivo, using artificially contaminated cockles with A. hydrophila ATCC 7966. In the in vitro experiments, all phages were effective against A. hydrophila, but phage AH-1 (with a maximum reduction of 7.7 log colonies forming units CFU/mL) was more effective than phages AH-4 and AH-5 (with reductions of 4.9 and 4.5 log CFU/mL, respectively). The cocktails AH-1/AH-4, AH-1/AH-5, AH-4/AH-5, and AH-1/AH-4/AH-5 were slightly more effective than the single phage suspensions. The phages presented a low emergence rate of phage-resistant mutants. When artificially contaminated cockles were treated in static seawater with phage AH-1, around 44% of the added A. hydrophila (1.0 log CFU/g) was inactivated. The results of this study suggest that phage therapy can be an effective alternative to control human pathogenic bacteria during depuration.
Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives
Authors: Orlicka-Płocka Marta , Fedoruk-Wyszomirska Agnieszka , Gurda-Woźna Dorota , Pawelczak Paweł , Krawczyk Patrycja , Giel-Pietraszuk Małgorzata , Framski Grzegorz , Ostrowski Tomasz , Wyszko Eliza
Published: June 2021

Recently, small compound-based therapies have provided new insights into the treatment of glioblastoma multiforme (GBM) by inducing oxidative impairment. Kinetin riboside (KR) and newly designed derivatives (8-azaKR, 7-deazaKR) selectively affect the molecular pathways crucial for cell growth by interfering with the redox status of cancer cells. Thus, these compounds might serve as potential alternatives in the oxidative therapy of GBM. The increased basal levels of reactive oxygen species (ROS) in GBM support the survival of cancer cells and cause drug resistance. The simplest approach to induce cell death is to achieve the redox threshold and circumvent the antioxidant defense mechanisms. Consequently, cells become more sensitive to oxidative stress (OS) caused by exogenous agents. Here, we investigated the effect of KR and its derivatives on the redox status of T98G cells in 2D and 3D cell culture. The use of spheroids of T98G cells enabled the selection of one derivative—7-deazaKR—with comparable antitumor activity to KR. Both compounds induced ROS generation and genotoxic OS, resulting in lipid peroxidation and leading to apoptosis. Taken together, these results demonstrated that KR and 7-deazaKR modulate the cellular redox environment of T98G cells, and vulnerability of these cells is dependent on their antioxidant capacity.
Impact of Hydrogen Peroxide on Protein Synthesis in Yeast
Authors: Picazo Cecilia , Molin Mikael
Published: June 2021

Cells must be able to respond and adapt to different stress conditions to maintain normal function. A common response to stress is the global inhibition of protein synthesis. Protein synthesis is an expensive process consuming much of the cell’s energy. Consequently, it must be tightly regulated to conserve resources. One of these stress conditions is oxidative stress, resulting from the accumulation of reactive oxygen species (ROS) mainly produced by the mitochondria but also by other intracellular sources. Cells utilize a variety of antioxidant systems to protect against ROS, directing signaling and adaptation responses at lower levels and/or detoxification as levels increase to preclude the accumulation of damage. In this review, we focus on the role of hydrogen peroxide, H2O2, as a signaling molecule regulating protein synthesis at different levels, including transcription and various parts of the translation process, e.g., initiation, elongation, termination and ribosome recycling.
Metabolism of 2,3-Dehydrosilybin A and 2,3-Dehydrosilybin B: A Study with Human Hepatocytes and Recombinant UDP-Glucuronosyltransferases and Sulfotransferases
Authors: Vrba Jiří , Papoušková Barbora , Lněničková Kateřina , Kosina Pavel , Křen Vladimír , Ulrichová Jitka
Published: June 2021

2,3-Dehydrosilybin A and 2,3-dehydrosilybin B are a pair of enantiomers formed by the oxidation of the natural flavonolignans silybin A and silybin B, respectively. However, the antioxidant activity of 2,3-dehydrosilybin molecules is much stronger than that of their precursors. Here, we investigated the biotransformation of pure 2,3-dehydrosilybin A and 2,3-dehydrosilybin B in isolated human hepatocytes, and we also aimed to identify human UDP-glucuronosyltransferases (UGTs) and sulfotransferases (SULTs) with activity toward their respective enantiomers. After incubation with hepatocytes, both 2,3-dehydrosilybin A and 2,3-dehydrosilybin B were converted to hydroxyl derivatives, methylated hydroxyl derivatives, methyl derivatives, sulfates, and glucuronides. The products of direct conjugations predominated over those of oxidative metabolism, and glucuronides were the most abundant metabolites. Furthermore, we found that recombinant human UGTs 1A1, 1A3, 1A7, 1A8, 1A9, and 1A10 were capable of catalyzing the glucuronidation of both 2,3-dehydrosilybin A and 2,3-dehydrosilybin B. UGTs 1A1 and 1A7 showed the highest activity toward 2,3-dehydrosilybin A, and UGT1A9 showed the highest activity toward 2,3-dehydrosilybin B. The sulfation of 2,3-dehydrosilybin A and B was catalyzed by SULTs 1A1*1, 1A1*2, 1A2, 1A3, 1B1, 1C2, 1C4, and 1E1, of which SULT1A3 exhibited the highest activity toward both enantiomers. We conclude that 2,3-dehydrosilybin A and B are preferentially metabolized by conjugation reactions, and that several human UGT and SULT enzymes may play a role in these conjugations.
Online Critical Unit Detection and Power System Security Control: An Instance-Level Feature Importance Analysis Approach
Authors: Ren Junyu , Wang Li , Zhang Shaofan , Cai Yanchun , Chen Jinfu
Published: June 2021

Rapid and accurate detection of critical units is crucial for the security control of power systems, ensuring reliable and continuous operation. Inspired by the advantages of data-driven techniques, this paper proposes an integrated deep learning framework of dynamic security assessment, critical unit detection, and security control. In the proposed framework, a black-box deep learning model is utilized to evaluate the dynamic security of power systems. Then, the predictions of the model for specific operating conditions are interpreted by instance-level feature importance analysis. Furthermore, the critical units are detected by reasonable local interpretation, and the security control scheme is extracted with a sequential adjustment strategy according to the results of interpretation. The numerical simulations on the CEPRI36 benchmark system and the IEEE 118-bus system verified that our proposed framework is fast and accurate for specific operating conditions and, thereby, is a viable approach for online security control of power systems.
Influence of Preparation Conditions on the Catalytic Performance of Mo/H-ZSM-5 for Methane Dehydroaromatization
Authors: Portilla Maria Teresa , Llopis Francisco J. , Moliner Manuel , Martinez Cristina
Published: June 2021

Methane, the main component of natural gas, is an interesting source of chemicals and clean liquid fuels, and a promising alternative raw material to oil. Among the possible direct routes for methane conversion, its aromatization under non-oxidative conditions has received increasing attention, despite the low conversions obtained due to thermodynamic limitations, because of its high selectivity to benzene. Mo/H-ZSM-5, the first bifunctional zeolite-catalyst proposed for this reaction, is still considered as one of the most adequate and has been widely studied. Although the mono- or bifunctional nature of the MDA mechanism is still under debate, it is generally accepted that the Mo species activate the C-H bond in methane, producing the intermediates. These will aromatize on the Brønsted acid sites of the zeolite, whose pore dimensions will provide the shape selectivity needed for converting methane into benzene. An additional role of the zeolite’s Brønsted acid sites is to promote the dispersion of the Mo oxide precursor. Here, we show the influence of the different preparation steps—metal incorporation, calcination and activation of the Mo/ZSM-5- on the metal dispersion and, therefore, on the activity and selectivity of the final catalyst. Metal dispersion is enhanced when the samples are calcined under dynamic conditions (DC) and activated in N2, and the benefits are larger when the metal has been incorporated by solid state reaction (SSR), as observed by FESEM-BSE and H2-TPR. This leads to catalysts with higher activity, increased aromatic selectivity and improved stability towards deactivation.
Mass Data Measurement, Approximation and Influence on Vehicle Stability for Ultra-Light Human-Powered Vehicles
Authors: Wilhelm Toni , Dorsch Volker , Gauterin Frank
Published: June 2021

The mass properties of a vehicle play a decisive role in its dynamics and characteristics and are fundamental for vehicle dynamics models and controllers. These values are not yet known for the vehicle class of the ultra-light velomobiles and similar multi-track bicycle vehicles. In the future, however, such vehicles could play a role in reducing the CO2 emissions generated by individual transportation. As a basis for vehicle dynamics modeling, accident reconstruction, and controller development for this vehicle class, this paper investigated ranges of mass properties and their influence on vehicle stability considering driver influence. In total, 13 vehicles (10 velomobiles and 3 trikes) were examined using different experimental setups. It was shown that most vehicles exhibited understeering behavior based on the center of gravity position and calculations of the static stability factor showed significantly lower rollover stability compared with conventional vehicles. The measured moments of inertia were used to develop and examine different approximation approaches for the yaw moment of inertia using conventional approaches from the passenger car sector and stepwise regression. This created the basis for parameter estimation from easily measurable vehicle parameters and provided the possibility to generate realistic parameter sets for vehicle dynamic models. Existing tests do not consider the influence of driver movements, such as pedaling movements or possible inclination of the upper body. This offers the potential for further investigations of the dynamic influences on the investigated variables.
Carbazole and Simplified Derivatives: Novel Tools toward β-Adrenergic Receptors Targeting
Authors: Grande Fedora , De Bartolo Anna , Occhiuzzi Maria Antonietta , Caruso Anna , Rocca Carmine , Pasqua Teresa , Carocci Alessia , Rago Vittoria , Angelone Tommaso , Sinicropi Maria Stefania
Published: June 2021

β-Adrenergic receptors (β-ARs) are G protein-coupled receptors involved in important physiological and pathological processes related to blood pressure and cardiac activity. The inhibition of cardiac β1-ARs could be beneficial in myocardial hypertrophy, ischemia and failure. Several carbazole-based compounds have been described as promising β-blockers. Herein, we investigate the capability of a carbazole derivative and three simplified indole analogs to interact with the active binding site of β1-AR by molecular docking studies. In the light of the obtained results, our compounds were tested by biological assays in H9c2 cardiomyocytes exposed to isoproterenol (ISO) to confirm their potential as β1-blockers agents, and two of them (8 and 10) showed interesting and promising properties. In particular, these compounds were effective against ISO-dependent in vitro cardiac hypertrophy, even at concentrations lower than the known β-AR antagonist propranolol. Overall, the data suggest that the indole derivatives 8 and 10 could act as potent β1-blockers and, active at low doses, could elicit limited side effects.
Model-Based Slippage Estimation to Enhance Planetary Rover Localization with Wheel Odometry
Authors: Gargiulo Anna Maria , di Stefano Ivan , Genova Antonio
Published: June 2021

The exploration of planetary surfaces with unmanned wheeled vehicles will require sophisticated software for guidance, navigation and control. Future missions will be designed to study harsh environments that are characterized by rough terrains and extreme conditions. An accurate knowledge of the trajectory of planetary rovers is fundamental to accomplish the scientific goals of these missions. This paper presents a method to improve rover localization through the processing of wheel odometry (WO) and inertial measurement unit (IMU) data only. By accurately defining the dynamic model of both a rover’s wheels and the terrain, we provide a model-based estimate of the wheel slippage to correct the WO measurements. Numerical simulations are carried out to better understand the evolution of the rover’s trajectory across different terrain types and to determine the benefits of the proposed WO correction method.
Potential Impacts of Climate Change on Shrimps Distribution of Commercial Importance in the Gulf of California
Authors: Cota-Durán Andres , Petatán-Ramírez David , Ojeda-Ruiz Miguel Ángel , Marín-Monroy Elvia Aida
Published: June 2021

The Gulf of California is the most productive fishing region in Mexico; its ecosystems contain a vast diversity of species with exploitation potential, some of them potentially vulnerable to climate change. This research was conducted to analyze, through habitat suitability models, the possible alterations in the distribution of the three shrimp species of the most importance for commercial fishing in the region: Litopenaeus stylirostris, Litopenaeus vannamei, and Farfantepenaeus californiensis. Habitat suitability models were built using the MaxEnt software, primary productivity data, temperature, salinity, bathymetry, substratum, coastal type, and geo-referenced occurrence records of the three species. Of the data, 70% was used on training, while the remaining 30% was used for validation. To make estimates of climate change impact on this fishery, projections on distribution of the three species from environmental forecasts generated by the intergovernmental panel on climate change until 2100 were made. The used model, that is in full development and expansion, could be considered as an applicable tool to other problems and showed efficiency rates above 90%. The species will maintain most of their historical distribution, but L. stylirostris and L. vannamei will have a new distribution area within the zones of the Magdalena-Almejas Bay and the Gulf of Ulloa, with an increase of 80% and 148% respectively; all species will have loss areas in the proportion of 16%, 2%, and 11%, respectively, along the southern Gulf of California.
Spatio-Temporal Variability in North Atlantic Oscillation Monthly Rainfall Signatures in Great Britain
Authors: West Harry , Quinn Nevil , Horswell Michael
Published: June 2021

The North Atlantic Oscillation (NAO) is the primary atmospheric-oceanic circulation/teleconnection influencing regional climate in Great Britain. As our ability to predict the NAO several months in advance increases, it is important that we improve our spatio-temporal understanding of the rainfall signatures that the circulation produces. We undertake a high resolution spatio-temporal analysis quantifying variability in rainfall response to the NAO across Great Britain. We analyse and map monthly NAO-rainfall response variability, revealing the spatial influence of the NAO on rainfall distributions, and particularly the probability of wet and dry conditions/extremes. During the winter months, we identify spatial differences in the rainfall response to the NAO between the NW and SE areas of Britain. The NW area shows a strong and more consistent NAO-rainfall response, with greater probability of more extreme wet/dry conditions. However, greater NAO-rainfall variability during winter was found in the SE. The summer months are marked by a more spatially consistent rainfall response; however, we find that there is variability in both wet/dry magnitude and directionality. We note the implications of these spatially and temporally variable NAO-rainfall responses for regional hydrometeorological predictions and highlight the potential explanatory role of other atmospheric-oceanic circulations.
Evaluation of HRCLDAS and ERA5 Datasets for Near-Surface Wind over Hainan Island and South China Sea
Authors: Jiang Yi , Han Shuai , Shi Chunxiang , Gao Tao , Zhen Honghui , Liu Xiaoyan
Published: June 2021

Near-surface wind data are particularly important for Hainan Island and the South China Sea, and there is a wide range of wind data sources. A detailed understanding of the reliability of these datasets can help us to carry out related research. In this study, the hourly near-surface wind data from the High-Resolution China Meteorological Administration (CMA) Land Data Assimilation System (HRCLDAS) and the fifth-generation ECMWF atmospheric reanalysis data (ERA5) were evaluated by comparison with the ground automatic meteorological observation data for Hainan Island and the South China Sea. The results are as follows: (1) the HRCLDAS and ERA5 near-surface wind data trend was basically the same as the observation data trend, but there was a smaller bias, smaller root-mean-square errors, and higher correlation coefficients between the near-surface wind data from HRCLDAS and the observations; (2) the quality of HRCLDAS and ERA5 near-surface wind data was better over the islands of the South China Sea than over Hainan Island land. However, over the coastal areas of Hainan Island and island stations near Sansha, the quality of the HRCLDAS near-surface wind data was better than that of ERA5; (3) the quality of HRCLDAS near-surface wind data was better than that of ERA5 over different types of landforms. The deviation of ERA5 and HRCLDAS wind speed was the largest along the coast, and the quality of the ERA5 wind direction data was poorest over the mountains, whereas that of HRCLDAS was poorest over hilly areas; (4) the accuracy of HRCLDAS at all wind levels was higher than that of ERA5. ERA5 significantly overestimated low-grade winds and underestimated high-grade winds. The accuracy of HRCLDAS wind ratings over the islands of the South China Sea was significantly higher than that over Hainan Island land, especially for the higher wind ratings; and (5) in the typhoon process, the simulation of wind by HRCLDAS was closer to the observations, and its simulation of higher wind speeds was more accurate than the ERA5 simulations.
Seasonality of the Airborne Ambient Soot Predominant Emission Sources Determined by Raman Microspectroscopy and Thermo-Optical Method
Authors: Zioła Natalia , Banasik Kamila , Jabłońska Mariola , Janeczek Janusz , Błaszczak Barbara , Klejnowski Krzysztof , Mathews Barbara
Published: June 2021

Raman microspectroscopy and thermo-optical-transmittance (TOT) method were used to study airborne ambient soot collected at the suburban air monitoring station in southern Poland during the residential heating (January-February) and non-heating (June–July) seasons of 2017. Carbonaceous material constituted on average 47.2 wt.% of PM2.5 during the heating season and 26.9 wt.% in the non-heating season. Average concentrations of OC (37.5 ± 11.0 μg/m3) and EC (5.3 ± 1.1 μg/m3) during the heating season were significantly higher than those in the non-heating season (OC = 2.65 ± 0.78 μg/m3, and EC = 0.39 ± 0.18 μg/m3). OC was a chief contributor to the TC mass concentration regardless of the season. All Raman parameters indicated coal combustion and biomass burning were the predominant sources of soot in the heating season. Diesel soot, which is structurally less ordered than soot from other sources, was dominant during the non-heating season. The D1 and G bands area ratio (D1A/GA) was the most sensitive Raman parameter that discriminated between various soot sources, with D1A/GA > 1 for diesel soot, and less than 1 for soot from coal and wood burning. Due to high daily variability of both TOT and Raman spectroscopy data, single-day measurements can be inconclusive regarding the soot source apportionment. Long-time measurement campaigns are recommended.
Ozonized Water in Microbial Control: Analysis of the Stability, In Vitro Biocidal Potential, and Cytotoxicity
Authors: Santos Laerte Marlon Conceição dos , Silva Eduardo Santos da , Oliveira Fabricia Oliveira , Rodrigues Leticia de Alencar Pereira , Neves Paulo Roberto Freitas , Meira Cássio Santana , Moreira Greta Almeida Fernandes , Lobato Gabriela Monteiro , Nascimento Carlos , Gerhardt Marcelo , Lessa Arlene Souza , Mascarenhas Luis Alberto Breda , Machado Bruna Aparecida Souza
Published: June 2021

O3 dissolved in water (or ozonized water) has been considered a potent antimicrobial agent, and this study aimed to test this through microbiological and in vitro assays. The stability of O3 was accessed following modifications of the physicochemical parameters of water, such as the temperature and pH, with or without buffering. Three concentrations of O3 (0.4, 0.6, and 0.8 ppm) dissolved in water were tested against different microorganisms, and an analysis of the cytotoxic effects was also conducted using the human ear fibroblast cell line (Hfib). Under the physicochemical conditions of 4 °C and pH 5, O3 remained the most stable and concentrated compared to pH 7 and water at 25 °C. Exposure to ozonized water resulted in high mortality rates for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Scanning electron micrograph images indicate that the effects on osmotic stability due to cell wall lysis might be one of the killing mechanisms of ozonized water. The biocidal agent was biocompatible and presented no cytotoxic effect against Hfib cells. Therefore, due to its cytocompatibility and biocidal action, ozonized water can be considered a viable alternative for microbial control, being possible, for example, its use in disinfection processes.
Periodontal Inflamed Surface Area Mediates the Link between Homocysteine and Blood Pressure
Authors: Botelho João , Machado Vanessa , Leira Yago , Proença Luís , Mendes José João
Published: June 2021

Here, we assess the association between homocysteine (Hcy) serum levels and periodontal status in a large representative sample of the National Health and Nutrition Examination Survey (NHANES). Using the 2001–2002 and 2003–2004 NHANES databases, participants with a periodontal examination, medical self-reported data, blood pressure (BP) and blood samples to determine complete blood count, C-reactive protein (CRP) and Hcy levels. We then calculated the periodontal inflamed surface area (PISA) and the periodontal epithelial surface area (PESA). Multivariable regression analysis explored the association between Hcy, periodontal measures and BP. Mediation analysis was performed to understand the effect of PISA and PESA in the link between Hcy and BP. 4021 participants fulfilled the inclusion criteria. Hcy levels showed significant correlations with systolic BP, diastolic BP, PISA, PESA and age. PESA showed to be significantly associated with Hcy both for the crude and adjusted models (p < 0.01), but not PISA (p > 0.05). In the association of Hcy with systolic BP, PISA significantly mediated 17.4% and PESA 0.9%. In the association of Hcy with diastolic BP, PISA significantly mediated 16.3% and PESA 47.2%. In conclusion, Hcy and periodontitis are associated. Further, both PISA and PESA significantly mediated the association of Hcy with systolic BP and diastolic BP. Future studies shall deepen the mechanisms by which Hcy levels increase in a clinical situation of periodontitis.
Amelioration of Chlorpyrifos-Induced Toxicity in Brassica juncea L. by Combination of 24-Epibrassinolide and Plant-Growth-Promoting Rhizobacteria
Authors: Bakshi Palak , Chouhan Rekha , Sharma Pooja , Mir Bilal Ahmad , Gandhi Sumit G. , Landi Marco , Zheng Bingsong , Sharma Anket , Bhardwaj Renu
Published: June 2021

Pervasive use of chlorpyrifos (CP), an organophosphorus pesticide, has been proven to be fatal for plant growth, especially at higher concentrations. CP poisoning leads to growth inhibition, chlorosis, browning of roots and lipid and protein degradation, along with membrane dysfunction and nuclear damage. Plants form a linking bridge between the underground and above-ground communities to escape from the unfavourable conditions. Association with beneficial rhizobacteria promotes the growth and development of the plants. Plant hormones are crucial regulators of basically every aspect of plant development. The growing significance of plant hormones in mediating plant–microbe interactions in stress recovery in plants has been extensively highlighted. Hence, the goal of the current study was to investigate the effect of 24-epibrassinolide (EBL) and PGPRs (Pseudomonas aeruginosa (Ma), Burkholderia gladioli (Mb)) on growth and the antioxidative defence system of CP-stressed Brassica juncea L. seedlings. CP toxicity reduced the germination potential, hypocotyl and radicle development and vigour index, which was maximally recuperated after priming with EBL and Mb. CP-exposed seedlings showed higher levels of superoxide anion (O2−), hydrogen peroxide (H2O2), lipid peroxidation and electrolyte leakage (EL) and a lower level of nitric oxide (NO). In-vivo visualisation of CP-stressed seedlings using a light and fluorescent microscope also revealed the increase in O2−, H2O2 and lipid peroxidation, and decreased NO levels. The combination of EBL and PGPRs reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) contents and improved the NO level. In CP-stressed seedlings, increased gene expression of defence enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APOX), glutathione peroxidase (GPOX), dehydroascorbate reductase (DHAR) and glutathione reductase (GPOX) was seen, with the exception of catalase (CAT) on supplementation with EBL and PGPRs. The activity of nitrate reductase (NR) was likewise shown to increase after treatment with EBL and PGPRs. The results obtained from the present study substantiate sufficient evidence regarding the positive association of EBL and PGPRs in amelioration of CP-induced oxidative stress in Brassica juncea seedlings by strengthening the antioxidative defence machinery.
Do Hot Executive Functions Relate to BMI and Body Composition in School Age Children?
Authors: Szcześniewska Paula , Hanć Tomasz , Bryl Ewa , Dutkiewicz Agata , Borkowska Aneta R. , Paszyńska Elżbieta , Słopień Agnieszka , Dmitrzak-Węglarz Monika
Published: June 2021

Deficits of ‘hot’ executive functions (EFs) involving emotional and motivational processes are considered as a risk factor for excessive weight, but few studies have tested the relationship between hot EFs and body composition in children. The aim of the study was to assess the association of the ability to delay gratification and affective decision-making with the body mass index (BMI) and body composition in children with typical neurocognitive development. The sample consisted of 553 Polish children aged between 6–12 y. The delay of gratification task (DGT) was applied to assess the ability to delay gratification. The Hungry Donkey test (HDT) was applied to assess affective decision-making. The indicators of decision-making in the HDT were net score and learning rate. The relationships between hot EFs and BMI, fat mass index (FMI), lean body mass index (LBMI) were tested. The association of the z scores of BMI and FMI, overweight/obesity, and the ability to delay gratification was found insignificant after controlling cofounding factors. Most of the results on affective decision-making and z scores for BMI, FMI and LBMI were insignificant as well. The relationship between the ability to delay gratification, affective decision-making, and adiposity is not pronounced in typically developed children.
From Fear to Hopelessness: The Buffering Effect of Patient-Centered Communication in a Sample of Oncological Patients during COVID-19
Authors: Rossi Alessandro Alberto , Marconi Maria , Taccini Federica , Verusio Claudio , Mannarini Stefania
Published: June 2021

Background: COVID-19 represents a threat both for the physical and psychological health of oncological patients experiencing heightened distress levels to which the fear of the virus is also added. Moreover, fear of COVID-19 could lead oncological patients to experience feelings of hopelessness related to their medical care. Patient-centered communication may act as a buffer against the aforementioned variables. This study aimed to test the role of doctor–patient communication in the relationship between fear of COVID-19 and hopelessness. Methods: During the COVID-19 pandemic, a sample of 90 oncological outpatients was recruited (40 males (44.4%) and 50 females (55.6%), mean age = 66.08 (SD = 12.12)). A structured interview was developed and used during the pandemic to measure the patients’ perceived (A) fear of COVID-19, and (B) feelings of hopelessness, and (C) physicians’ use of empathetic and (D) clear language during the consultation. A multiple mediation model was tested, and the effects between males and females were also compared. Results: Empathetic and clear doctor–patient communication buffered the adverse effect of the fear of COVID-19 on hopelessness through a full-mediation model. The effects did not differ between males and females in the overall model but its indirect effects. Discussions: Patient-centered communication using empathy and clear language can buffer the adverse effect of the fear of COVID-19 and protect oncological patients from hopelessness during the pandemic. These findings might help to improve clinical oncological practice.
Pulmonary Toxicity after Total Body Irradiation—An Underrated Complication? Estimation of Risk via Normal Tissue Complication Probability Calculations and Correlation with Clinical Data
Authors: Oertel Michael , Kittel Christopher , Martel Jonas , Mikesch Jan-Henrik , Glashoerster Marco , Stelljes Matthias , Eich Hans Theodor
Published: June 2021

Total body irradiation (TBI) is an essential part of various conditioning regimens prior to allogeneic stem cell transplantation, but is accompanied by relevant (long-term) toxicities. In the lungs, a complex mechanism induces initial inflammation (pneumonitis) followed by chronic fibrosis. The hereby presented analysis investigates the occurrence of pulmonary toxicity in a large patient collective and correlates it with data derived from normal tissue complication probability (NTCP) calculations. The clinical data of 335 hemato-oncological patients undergoing TBI were analyzed with a follow-up of 85 months. Overall, 24.8% of all patients displayed lung toxicities, predominantly pneumonia and pulmonary obstructions (13.4% and 6.0%, respectively). NTCP calculations estimated median risks to be 20.3%, 0.6% and 20.4% for overall pneumonitis (both radiological and clinical), symptomatic pneumonitis and lung fibrosis, respectively. These numbers are consistent with real-world data from the literature and further specify radiological and clinical apparent toxicity rates. Overall, the estimated risk for clinical apparent pneumonitis is very low, corresponding to the probability of non-infectious acute respiratory distress syndrome, although the underlying pathophysiology is not identical. Radiological pneumonitis and lung fibrosis are expected to be more common but require a more precise documentation by the transplantation team, radiologists and radiation oncologists.
Long Intergenic Non-Coding RNAs in HNSCC: From “Junk DNA” to Important Prognostic Factor
Authors: Kozłowska Joanna , Kolenda Tomasz , Poter Paulina , Sobocińska Joanna , Guglas Kacper , Stasiak Maciej , Bliźniak Renata , Teresiak Anna , Lamperska Katarzyna
Published: June 2021

Head and neck squamous cell carcinoma is one of the most common and fatal cancers worldwide. Even a multimodal approach consisting of standard chemo- and radiotherapy along with surgical resection is only effective in approximately 50% of the cases. The rest of the patients develop a relapse of the disease and acquire resistance to treatment. Especially this group of individuals needs novel, personalized, targeted therapy. The first step to discovering such solutions is to investigate the tumor microenvironment, thus understanding the role and mechanism of the function of coding and non-coding sequences of the human genome. In recent years, RNA molecules gained great interest when the complex character of their impact on our biology allowed them to come out of the shadows of the “junk DNA” label. Furthermore, long non-coding RNAs (lncRNA), specifically the intergenic subgroup (lincRNA), are one of the most aberrantly expressed in several malignancies, which makes them particularly promising future diagnostic biomarkers and therapeutic targets. This review contains characteristics of known and validated lincRNAs in HNSCC, such as XIST, MALAT, HOTAIR, HOTTIP, lincRNA-p21, LINC02487, LINC02195, LINC00668, LINC00519, LINC00511, LINC00460, LINC00312, and LINC00052, with a description of their prognostic abilities. Even though much work remains to be done, lincRNAs are important factors in cancer biology that will become valuable biomarkers of tumor stage, outcome prognosis, and contribution to personalized medicine.
Clinical Relevance of Viable Circulating Tumor Cells in Patients with Metastatic Colorectal Cancer: The COLOSPOT Prospective Study
Authors: Mazard Thibault , Cayrefourcq Laure , Perriard Françoise , Senellart Hélène , Linot Benjamin , de la Fouchardière Christelle , Terrebonne Eric , François Eric , Obled Stéphane , Guimbaud Rosine , Mineur Laurent , Fonck Marianne , Daurès Jean-Pierre , Ychou Marc , Assenat Eric , Alix-Panabières Catherine
Published: June 2021

Background: Circulating tumor cells (CTCs) allow the real-time monitoring of tumor course and treatment response. This prospective multicenter study evaluates and compares the early predictive value of CTC enumeration with EPISPOT, a functional assay that detects only viable CTCs, and with the CellSearch® system in patients with metastatic colorectal cancer (mCRC). Methods: Treatment-naive patients with mCRC and measurable disease (RECIST criteria 1.1) received FOLFIRI–bevacizumab until progression or unacceptable toxicity. CTCs in peripheral blood were enumerated at D0, D14, D28, D42, and D56 (EPISPOT assay) and at D0 and D28 (CellSearch® system). Progression-free survival (PFS) and overall survival (OS) were assessed with the Kaplan–Meier method and log-rank test. Results: With the EPISPOT assay, at least 1 viable CTC was detected in 21% (D0), 15% (D14), 12% (D28), 10% (D42), and 12% (D56) of 155 patients. PFS and OS were shorter in patients who remained positive, with viable CTCs between D0 and D28 compared with the other patients (PFS = 7.36 vs. 9.43 months, p = 0.0161 and OS = 25.99 vs. 13.83 months, p = 0.0178). The prognostic and predictive values of ≥3 CTCs (CellSearch® system) were confirmed. Conclusions: CTC detection at D28 and the D0–D28 CTC dynamics evaluated with the EPISPOT assay were associated with outcomes and may predict response to treatment.
Meta-Analysis of the Accuracy of Abbreviated Magnetic Resonance Imaging for Hepatocellular Carcinoma Surveillance: Non-Contrast versus Hepatobiliary Phase-Abbreviated Magnetic Resonance Imaging
Authors: Kim Dong Hwan , Choi Sang Hyun , Shim Ju Hyun , Kim So Yeon , Lee Seung Soo , Byun Jae Ho , Choi Joon-Il
Published: June 2021

We aimed to determine the performance of surveillance abbreviated magnetic resonance imaging (AMRI) for detecting hepatocellular carcinoma (HCC), and to compare the performance of surveillance AMRI according to different protocols. Original research studies reporting the performance of surveillance AMRI for the detection of HCC were identified in MEDLINE, EMBASE, and Cochrane databases. The pooled sensitivity and specificity of surveillance AMRI were calculated using a hierarchical model. The pooled sensitivity and specificity of contrast-enhanced hepatobiliary phase (HBP)-AMRI and non-contrast (NC)-AMRI were calculated and compared using bivariate meta-regression. Ten studies, including 1547 patients, reported the accuracy of surveillance AMRI. The pooled sensitivity and specificity of surveillance AMRI for detecting any-stage HCC were 86% (95% confidence interval (CI), 80–90%; I2 = 0%) and 96% (95% CI, 93–98%; I2 = 80.5%), respectively. HBP-AMRI showed a significantly higher sensitivity for detecting HCC than NC-AMRI (87% vs. 82%), but significantly lower specificity (93% vs. 98%) (p = 0.03). Study quality and MRI magnet field strength were factors significantly associated with study heterogeneity (p ≤ 0.01). In conclusion, surveillance AMRI showed good overall diagnostic performance for detecting HCC. HBP-AMRI had significantly higher sensitivity for detecting HCC than NC-AMRI, but lower specificity.
Application of TiO2-Based Photocatalysts to Antibiotics Degradation: Cases of Sulfamethoxazole, Trimethoprim and Ciprofloxacin
Authors: Kutuzova Anastasiya , Dontsova Tetiana , Kwapinski Witold
Published: June 2021

The extensive application of antibiotics in human and veterinary medicine has led to their widespread occurrence in a natural aquatic environment. Global health crisis is associated with the fast development of antimicrobial resistance, as more and more infectious diseases cannot be treated more than once. Sulfamethoxazole, trimethoprim and ciprofloxacin are the most commonly detected antibiotics in water systems worldwide. The persistent and toxic nature of these antibiotics makes their elimination by conventional treatment methods at wastewater treatment plants almost impossible. The application of advanced oxidation processes and heterogeneous photocatalysis over TiO2-based materials is a promising solution. This highly efficient technology has the potential to be sustainable, cost-efficient and energy-efficient. A comprehensive review on the application of various TiO2-based photocatalysts for the degradation of sulfamethoxazole, trimethoprim and ciprofloxacin is focused on highlighting their photocatalytic performance under various reaction conditions (different amounts of pollutant and photocatalyst, pH, light source, reaction media, presence of inorganic ions, natural organic matter, oxidants). Mineralization efficiency and ecotoxicity of final products have been also considered. Further research needs have been presented based on the literature findings. Among them, design and development of highly efficient under sunlight, stable, recyclable and cost-effective TiO2-based materials; usage of real wastewaters for photocatalytic tests; and compulsory assessment of products ecotoxicity are the most important research tasks in order to meet requirements for industrial application.
Lymphocytes and Trogocytosis-Mediated Signaling
Authors: Reed Jim , Reichelt Madison , Wetzel Scott A.
Published: June 2021

Trogocytosis is the intercellular transfer of membrane and membrane-associated molecules. This underappreciated process has been described in a variety of biological settings including neuronal remodeling, fertilization, viral and bacterial spread, and cancer, but has been most widely studied in cells of the immune system. Trogocytosis is performed by multiple immune cell types, including basophils, macrophages, dendritic cells, neutrophils, natural killer cells, B cells, γδ T cells, and CD4+ and CD8+ αβ T cells. Although not expressed endogenously, the presence of trogocytosed molecules on cells has the potential to significantly impact an immune response and the biology of the individual trogocytosis-positive cell. Many studies have focused on the ability of the trogocytosis-positive cells to interact with other immune cells and modulate the function of responders. Less understood and arguably equally important is the impact of these molecules on the individual trogocytosis-positive cell. Molecules that have been reported to be trogocytosed by cells include cognate ligands for receptors on the individual cell, such as activating NK cell ligands and MHC:peptide. These trogocytosed molecules have been shown to interact with receptors on the trogocytosis-positive cell and mediate intracellular signaling. In this review, we discuss the impact of this trogocytosis-mediated signaling on the biology of the individual trogocytosis-positive cell by focusing on natural killer cells and CD4+ T lymphocytes.
The Role of Irisin in Cancer Disease
Authors: Pinkowska Agnieszka , Podhorska-Okołów Marzenna , Dzięgiel Piotr , Nowińska Katarzyna
Published: June 2021

Irisin (Ir) is an adipomyokine that is involved in the regulation of metabolic processes. It also influences processes related to inflammation, including cancer. Initially, Ir was considered a hormone secreted by skeletal muscles in response to physical exercise. Further studies showed that Ir is also present in other healthy tissues, organs, and plasma. It influences the change in phenotype of white adipose tissue (WAT) into brown adipose tissue (BAT). It increases mitochondrial biogenesis and affects the expression of thermogenin (UCP1). This adipomyokine has also been found in many tumor tissues and in the serum of cancer patients. Studies are underway to determine the association between Ir and carcinogenesis. It has been confirmed that Ir inhibits in vitro proliferation, migration, and invasion. It is involved in the inhibition of epithelial–mesenchymal transition (EMT). Additionally, Ir affects the expression of the transcription factor Snail, which is involved in EMT, and inhibits transcription of the gene encoding E-cadherin, which is characteristic of epithelial-derived cells. Many studies have been performed to determine the role of Ir in physiological and pathological processes. Further detailed studies should determine more precisely the effect of Ir on the body in health and disease.
Features of the Duckweed Lemna That Support Rapid Growth under Extremes of Light Intensity
Authors: Stewart Jared J. , Adams William W. , López-Pozo Marina , Doherty Garcia Naiara , McNamara Maureen , Escobar Christine M. , Demmig-Adams Barbara
Published: June 2021

This study addresses the unique functional features of duckweed via comparison of Lemna gibba grown under controlled conditions of 50 versus 1000 µmol photons m−2 s−1 and of a L. minor population in a local pond with a nearby population of the biennial weed Malva neglecta. Principal component analysis of foliar pigment composition revealed that Malva was similar to fast-growing annuals, while Lemna was similar to slow-growing evergreens. Overall, Lemna exhibited traits reminiscent of those of its close relatives in the family Araceae, with a remarkable ability to acclimate to both deep shade and full sunlight. Specific features contributing to duckweed’s shade tolerance included a foliar pigment composition indicative of large peripheral light-harvesting complexes. Conversely, features contributing to duckweed’s tolerance of high light included the ability to convert a large fraction of the xanthophyll cycle pool to zeaxanthin and dissipate a large fraction of absorbed light non-photochemically. Overall, duckweed exhibited a combination of traits of fast-growing annuals and slow-growing evergreens with foliar pigment features that represented an exaggerated version of that of terrestrial perennials combined with an unusually high growth rate. Duckweed’s ability to thrive under a wide range of light intensities can support success in a dynamic light environment with periodic cycles of rapid expansion.