bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing Bitumen Performance in Warm Mix Asphalt Using Cecabase RT BIO10: A Taguchi-Based Experimental Approach
Authors: Çakı Mustafa , Baş Fatih İrfan
Published: February 2025

Flexible pavements stand out as the most commonly used worldwide, compared to rigid and composite pavements, owing to their versatility and widespread application. The use of hot mix asphalt (HMA) in flexible pavements causes significant environmental concerns due to high CO2 emissions and energy consumption, whereas warm mix asphalt (WMA) technologies have gained popularity in recent decades, offering a more sustainable alternative by enabling asphalt production at lower temperatures. WMA technologies can be categorized into three main groups: foaming, organic additives, and chemical additives, with each offering distinct benefits for performance and environmental impact. One of the chemical additives used in WMA production is Cecabase RT BIO10. In this study, virgin bitumen with 50/70 penetration was modified by adding Cecabase RT BIO10 at four levels: 0%, 0.3%, 0.4%, and 0.5% by weight. The experimental design employed a Taguchi L16 orthogonal array to systematically evaluate the effects of various factors on modified bitumen performance. Binders were prepared at four temperatures (110 °C, 120 °C, 130 °C, and 140 °C), four mixing durations (15, 20, 25, and 30 min), and four mixing speeds (1000, 2000, 3000, and 4000 rpm), enabling an efficient analysis of each parameter’s impact. The prepared binders were subjected to a series of tests, including penetration, softening point, flash point, rotational thin film oven test (RTFOT), elastic recovery, Marshall stability, ultrasonic pulse velocity (UPV), and FTIR analysis. These tests were conducted to investigate the effects of various parameters and levels on the binder properties. Additionally, stiffness and seismic modules were evaluated to provide a more comprehensive understanding of the binder’s performance. The experiment results revealed that the penetration, elastic recovery percentage, and Marshall stability increased with increasing additive content while the softening point and RTFOT mass loss decreased. At a high service temperature of 40 °C, the stiffness modulus of the modified bitumen decreased slightly. At a low service temperature of −10 °C, it decreased further. Additionally, the incorporation of Cecabase RT BIO10 led to an increase in the seismic modulus. Through optimization using the Taguchi method, the optimal levels were determined to be a 0.4% Cecabase RT BIO10 ratio, 140 °C mixing temperature, 30 min mixing time, and 1000 RPM mixing speed. The optimal responses for each test were identified and integrated into a unified optimal response, resulting in a comprehensive design guide with 95% confidence level estimates for all possible level combinations.
Study of Concrete Moisture Transfer Characteristics in the Presence of the Concrete Micro–Meso Structure Effect
Authors: Zhang Xiaogang , Zhang Shuhua , Chen Bofu , Tian Bin , Lu Xiaochun , Xiong Bobo , Pan Zhao
Published: February 2025

Water and water transfer are the keys of the concrete durability problem; the non-uniform moisture transfer caused by the concrete micro–meso structure has a great effect on the drying shrinkage crack, transfers of inimical ions, etc. For the non-uniform moisture transfer problem, a multi-scale concrete moisture diffusion coefficient model which can consider the effect of Knudsen diffusion was established and verified based on the moisture transfer mechanism of porous medium and the concrete micro–meso structure characteristics. The effects of pore structure, the interfacial transition zone, and aggregate on the concrete moisture diffusion coefficient were studied based on the model, and the non-uniform moisture transfer characteristics and differences in concrete wetting and drying were analyzed via simulations. The results show that the moisture transfers more easily via the pores ranging from 10 nm to 100 nm, the effect of Knudsen diffusion increases with the increasing water-to-cement ratio and decreases with the increasing relative moisture, and Knudsen diffusion is also an effect factor which causes the moisture diffusion coefficient to increase with the increase in moisture. Moisture transfers more easily via the interfacial transition zone at the meso-level and causes a “flow around” phenomenon. The “S” growth relation between the moisture diffusion coefficient and relative moisture can consider the differences in the moisture diffusion coefficient under wetting and drying conditions to a certain extent, which makes concrete wet faster than dry. In addition, the jumping growth of the moisture diffusion coefficient in the relation also leads to an “inflection point” in the concrete moisture distribution.
Study on Delay Time and Rock Mass Damage Patterns in Pre-Split Blasting of Water-Saturated Skarn in Alpine Regions
Authors: Liu Jinbao , Li Xianglong , Tai Chengqun , Hu Tao , Xu Jie
Published: February 2025

In order to solve the problem of ineffective pre-splitting blasting and reduce the damage caused to preserved rock bodies in the Niukutou open-pit quarry in the alpine region of Qinghai Province, China, this study investigated the influence of different delay times on the damage caused to the rock mass, combining numerical calculations with field tests. Using the finite element software ANSYS/LS-DYNA 19.0 and based on the constitutive model of saturated skarn, numerical models of pre-splitting blasting with four different delay times (0 ms, 12 ms, 18 ms, and 24 ms) were established. These models systematically analyze the damage evolution of rock and the effective stress distribution in specific elements under different delay times. The results indicate that a maximum damage depth of 32.56 cm occurs when the inter-hole delay is 0 ms, while a minimum damage depth of 30.61 cm is observed when the inter-hole delay is 18 ms. Further analysis reveals that the peak effective stress is highest when the inter-hole delay is 0 ms, and that the peak effective stress at 18 ms is higher than that at 12 ms and 24 ms. Considering the need to control the damage caused to the surrounding rock and the blasting effectiveness, the best pre-splitting blasting effect is achieved with an inter-hole delay of 18 ms. Field tests show that the damage depth of the retained rock mass is 1.62 m when the inter-hole delay is 0 ms; however, it decreases to 0.90 m when the delay is 18 ms, representing a 44% reduction in rock mass damage depth. This study provides theoretical support and practical guidance for the optimization of pre-splitting blasting in saturated skarn in alpine regions.
A Body–Machine Interface for Assistive Robot Control in Spinal Cord Injury: System Description and Preliminary Tests
Authors: Freccero Aurora , Feder Maddalena , Grioli Giorgio , Catalano Manuel Giuseppe , Massone Antonino , Bicchi Antonio , Casadio Maura
Published: February 2025

Motor impairments, particularly spinal cord injuries, impact thousands of people each year, resulting in severe sensory and motor disabilities. Assistive technologies play a crucial role in supporting these individuals with activities of daily living. Among such technologies, body–machine interfaces (BoMIs) are particularly important, as they convert residual body movements into control signals for external robotic devices. The main challenge lies in developing versatile control interfaces that can adapt to the unique needs of individual users. This study aims to adapt for people with spinal cord injury a novel control framework designed to translate residual user movements into commands for the humanoid robot Alter-Ego. After testing and refining the control algorithm, we developed an experimental protocol to train users to control the robot in a simulated environment. A total of 12 unimpaired participants and two individuals affected by spinal cord injury participated in this study, which was designed to assess the system’s applicability and gather end-user feedback on its performance in assisting with daily tasks. Key metrics such as the system’s usability, accuracy, performance, and improvement metrics in navigation and reaching tasks were assessed. The results suggest that assistive robots can be effectively controlled using minimal residual movements. Furthermore, structured training sessions significantly enhance overall performance and improve the accuracy of the control algorithm across the selected tasks.
An Analytical Solution for Natural Frequencies of Elastically Supported Stepped Beams with Rigid Segments
Authors: Kostekci Ferid
Published: February 2025

In this work, an analytical solution for the natural frequencies of elastically supported stepped beams with rigid segments is presented. The elastic end boundary conditions are modeled with a translational stiffness element, a rotational stiffness element, and an end-concentrated mass. This model is of great significance in machine construction studies. Under the assumption of Euler–Bernoulli beam theory, the non-dimensional equations of the motion and main equations that can give all of the boundary conditions were obtained by using Hamilton’s principle. After deriving the transverse displacement functions by means of using the separation-of-variables technique, the frequency equation was found by setting the determinant of the coefficient matrix to zero. The natural frequencies of the transverse vibrations were found according to physical and geometric parameters. The method was validated by using FEM results and findings from the literature. This study indicates that the physical and geometric parameters of the elastic supports and rigid segments affect the natural frequencies of the beam. The revealed analytical method can be used to calculate the natural frequencies and mode shapes of all beam types, such as elastically supported uniform beams and single-step beams with or without concentrated mass and/or rigid segments.
Assessment of Corn Grain Production Under Drought Conditions in Eastern Mexico Through the North American Drought Monitor
Authors: Valdés-Rodríguez Ofelia Andrea , Salas-Martínez Fernando , Palacios-Wassenaar Olivia , Marquez Aldo
Published: February 2025

Over 80% of corn on Mexico’s eastern side is sown under rainfed conditions. Therefore, drought represents a constant challenge for local producers. This study aims to determine the effects of drought on rainfed corn grain production on Mexico’s eastern side by using the North American Drought Monitor as the primary tool. Drought levels at the municipal level provided by this monitor and corn production data (surface damage, yield, and volume) of the two productive seasons (spring–summer and autumn–winter) during 20 years were correlated at two significant levels (0.05 and 0.01). The significant values (p < 0.05) were used to obtain regression curves representing corn-drought behaviors. The National Disaster Statistics and climatological stations were considered, discarding other phenomena besides drought. Results indicate that, for the significant municipalities, the years with the highest drought levels (2005, 2011, and 2019) positively correlate with reduced corn grain yield, volume, and total harvest losses. The regression curves estimated a yield reduction of 78 kg∙ha−1 during the spring–summer season and 76 kg∙ha−1 during the autumn–winter season. We concluded that the Drought Monitor is valuable for determining relationships between rainfed corn grain productivity and drought, considering that no other climatological phenomena affect the region.
Effect of Washing Method and Intake Concentration on the Removal of Odor and Particulate Matter
Authors: Li Yexi , Liu Zhize , Zhu Qubo , Zhu Haisheng
Published: February 2025

This study investigates the effects of different washing methods and intake concentrations on the removal efficiency of odor gases and particulate matter. Odor concentration (OU value) was used as the evaluation criterion, with three concentration gradients established: high (1000–1100 OU), medium (500–600 OU), and low (200–300 OU). The experiment employed water washing, acidic washing (pH = 4), and alkaline washing (pH = 10) to analyze the removal rates across nine treatments. Results indicated that the removal rate of ammonia significantly increased with higher intake concentrations (p < 0.01), while intake concentration had no significant effect on the removal rates of hydrogen sulfide and volatile organic compounds (VOCs). Acidic washing achieved the highest ammonia removal rate (72.68%) (p < 0.01), whereas alkaline washing showed the highest hydrogen sulfide removal rate (64.87%) (p < 0.01). For VOCs, the removal rates for water washing, acidic washing, and alkaline washing were 50.35%, 59.70%, and 61.56%, respectively, which were significantly lower than those for acidic and alkaline washing (p < 0.01). Alkaline washing demonstrated a significantly higher removal rate for methyl mercaptan compared to water washing and acidic washing (p < 0.01), and also for dimethyl sulfide compared to acidic washing (p < 0.05). Trimethylamine and carbon disulfide removal rates by acidic and alkaline washing were significantly higher than those by water washing (p < 0.01). There was no significant difference in styrene removal rates among different washing methods (p > 0.05), although acidic washing showed the best performance. Neither washing method nor intake concentration significantly affected particulate matter removal. It is recommended to combine acidic and alkaline washing for optimal deodorization.
High-Resolution Fourier Transform Spectra of Atomic Sulfur: Testing of Modified Quantum Defect Theory
Authors: Chernov Vladislav E. , Manakov Nikolai L. , Meremianin Alexei V. , Naskidashvili Alexander V. , Civiš Svatopluk , Ferus Martin , Kubelík Petr , Zanozina Ekaterina M. , Zetkina Oxana V.
Published: February 2025

QDT (quantum defect theory) is an effective technique for calculating processes involving highly excited (Rydberg) states of atoms, ions, and molecules with one valence electron outside filled shells, whose spectrum generally resembles a hydrogen-like atom’s spectrum. At the expense of some modification of QDT, in this paper, we extend its applicability to describe low- and intermediate-excited levels of atoms with more complex spectra (on the example of atomic sulfur S I). Transitions between just such states are responsible for the infrared (IR) spectra of atoms. While the quantum defects (QDs) of the highly excited Rydberg levels are determined by the energies of individual levels near the ionization threshold, the radial wave functions of low excited electronic states, in the framework of our modification of QDT, include the QD dependence on energy over a wide energy range; this dependence is determined from the whole spectral series. We show that, outside the atomic core domain, the electron radial functions calculated using modified semi-phenomenological QDT agree well with ab initio calculations. As another assessment of QDT accuracy, we show satisfactory agreement of the probabilities of dipole transitions in S I, taken from the NIST Atomic Spectra Database, with our QDT calculations. We perform an indirect experimental verification of QDT on the basis of spectra of S I in gas-discharge plasma measured by time-resolved high-resolution Fourier transfer spectroscopy (FTS). The Boltzmann plot built from our measured spectra demonstrates that QDT provides a satisfactory approximation for calculating the experimental lines’ intensities.
Proximal Point Methods for Solving Equilibrium Problems in Hadamard Spaces
Authors: Djafari Rouhani Behzad , Mohebbi Vahid
Published: February 2025

We investigate the Δ-convergence and strong convergence of a sequence generated by the proximal point method for pseudo-monotone equilibrium problems in Hadamard spaces. First, we show the Δ-convergence of the generated sequence to a solution of the equilibrium problem. Next, we prove the strong convergence of the generated sequence with some additional conditions imposed on the bifunction. Finally, we prove the strong convergence of the generated sequence, by using Halpern’s regularization method, without any additional condition.
Patellar Dislocation Patients Had Lower Bone Mineral Density and Hounsfield Unit Values in the Knee Joint Compared to Patients with Anterior Cruciate Ligament Ruptures: A Focus on Cortical Bone in the Tibia
Authors: Wu Yue , Wang Yiting , Wang Haijun , Jia Shaowei , Ao Yingfang , Gong Xi , Liu Zhenlong
Published: February 2025

Anterior cruciate ligament (ACL) rupture and patellar dislocation (PD) are common knee injuries. Dual-energy X-ray absorptiometry (DXA) and computed tomography (CT) are widely used clinical diagnostic tools. The aim was to investigate the characteristics of knee bone mineral density (BMD) in patients with ACL rupture and PD and to explore the relationship between BMD and Hounsfield unit (HU) values. This prospective cross-sectional study included 32 ACL rupture and 32 PD patients assessed via DXA and CT. BMD and CT measurements were taken from regions of interest in the femoral and tibial condyles. Statistical analyses included t-tests and mixed-effects models. The results showed that BMD in the PD group was significantly lower than in the ACL group (p < 0.05). The HU values of cortical bone in the femur and tibia differed significantly between the ACL group and the PD group (p < 0.05). The BMD of the femur and tibia showed significant correlations with the HU values of cancellous bone and cortical bone (p < 0.05). The conclusion was that PD patients had lower BMD and HU values in the femur and tibia compared to patients with ACL ruptures, particularly in the cortical bone of the tibia, and there was a strong correlation between HU value and BMD.
Integrated Transcriptome and Proteome Analysis Provides New Insights into Starch and Sucrose Metabolism and Regulation of Corm Expansion Process in Colocasia esculenta
Authors: Zou Chengwu , He Fanglian , Li Huinan , Liu Lili , Qiu Zuyang , Dong Weiqing
Published: February 2025

The global significance of Colocasia esculenta, a tuber crop rich in nutritional value and starch, prompts further investigation into its corm development. Background: Previous studies have focused on starch accumulation within the tubers, yet the genetic and proteomic basis of corm expansion remains largely unexplored. This study aims to elucidate the key genes and proteins involved in this process. Methods: We selected ‘Lipu Taro No.1’ and conducted a longitudinal starch content analysis, full-length transcriptome sequencing, and a proteomic analysis during three distinct stages of corm development. Results: Our findings reveal a significant increase in both amylose and amylopectin contents as the corm develops, indicating the temporal regulation of starch biosynthesis. The integration of transcriptome and proteomic data identified differentially expressed genes and proteins associated with starch and sucrose metabolism, as well as plant hormone signal transduction. Conclusions: This study delineates a temporal gene expression pattern that is crucial for starch synthesis and provides insights into the regulatory mechanisms controlling corm expansion and starch deposition, offering valuable references for future molecular breeding strategies to enhance taro yield and quality.
The Role of Non-Human Sialic Acid Neu5Gc-Containing Glycoconjugates in Human Tumors: A Review of Clinical and Experimental Evidence
Authors: Blanco Rancés , Muñoz Juan P.
Published: February 2025

N-Glycolylneuraminic acid (Neu5Gc) is a sialic acid variant commonly found in most mammals but not synthesized by humans due to an inactivating mutation in the CMP-Neu5Ac hydroxylase (CMAH) gene. Despite this, Neu5Gc-containing molecules are consistently detected in human tissues, particularly in malignant tumors. However, the mechanisms underlying Neu5Gc accumulation and its role in cancer development remain poorly understood. Objectives: This review aims to analyze clinical and experimental evidence regarding the presence of Neu5Gc-containing glycoconjugates in both tumor and non-tumor human tissues, exploring potential mechanisms of the Neu5Gc expression and evaluating its contribution to tumor biology, with a particular focus on the Neu5Gc-GM3 ganglioside. Methods: A comprehensive review of the literature was conducted, integrating findings from immunohistochemistry, chromatography, and molecular studies to assess the expression and implications of Neu5Gc in cancer biology. Results: Neu5Gc-containing glycoconjugates were found to preferentially accumulate in various malignant tumors, while their presence in normal tissues was restricted to cells with high turnover rates. This accumulation is potentially mediated by dietary uptake, hypoxic conditions, and metabolic alterations in cancer cells. Additionally, Neu5Gc-containing molecules were associated with the activation of oncogenic pathways. Conclusion: Neu5Gc-containing glycoconjugates play a multifaceted role in cancer progression and present potential as prognostic markers and therapeutic targets.
Enhanced Site-Specific Fluorescent Labeling of Membrane Proteins Using Native Nanodiscs
Authors: Ezsias Bence , Wolkenstein Felix , Goessweiner-Mohr Nikolaus , Yadav Rohit , Siligan Christine , Posch Sandra , Horner Andreas , Vargas Carolyn , Keller Sandro , Pohl Peter
Published: February 2025

Fluorescent labeling of membrane proteins is essential for exploring their functions, signaling pathways, interaction partners, and structural dynamics. Organic fluorophores are commonly used for this purpose due to their favorable photophysical properties and photostability. However, a persistent challenge is the inaccessibility of the surface-exposed cysteine residues required for site-specific labeling, as these residues often become sequestered within detergent micelles during protein extraction. To address this limitation, we developed an approach based on polymer-encapsulated nanodiscs that preserves the protein’s native-like lipid-bilayer environment while ensuring the accessibility of surface-exposed cysteine residues. In this method, His-tagged proteins embedded in native nanodiscs are retained on a nickel affinity column, allowing for simultaneous purification and labeling by adding fluorescent dyes. This versatile technique was demonstrated with two challenging-to-label membrane proteins, the potassium channel KvAP and the urea channel HpUreI, for which detergent-based labeling had failed. This opens new possibilities for studying a wide range of fluorescently labeled membrane proteins in near-native states, advancing applications in biophysics, structural biology, and drug discovery.
Comparison of End-Tidal Carbon Dioxide Values in ICU Patients with and Without In-Hospital Cardiac Arrest
Authors: Dalton Kaitlyn , Mucksavage Jeffrey J. , Fraidenburg Dustin R. , He Kevin , Chang James , Panlilio-Villanueva Maria , Wang Tianxiu , Benken Scott T.
Published: February 2025

Objective: The purpose of this study was to evaluate the utility of end-tidal carbon dioxide (ETCO2) values as a predictive marker of in-hospital cardiac arrest (IHCA). This was achieved by comparing the trends of ETCO2 values in mechanically ventilated ICU patients that experienced an IHCA versus patients that did not. Methods: A single-center, retrospective, observational, and comparative cohort study at an academic medical center. Mechanically ventilated adults in the ICU who received continuous ETCO2 monitoring were included. Patients who were transferred to our facility already intubated, experienced an out-of-hospital cardiac arrest, or had a do-not-resuscitate order were excluded. Extracted data points included demographics, comorbidities, vitals, labs, and outcomes. Patients were grouped into IHCA and non-IHCA cohorts, and the trends of ETCO2 values were compared at multiple time points for 48 h before the IHCA or after intubation (time zero) for the groups, respectively. An ROC curve was constructed to determine the predictive value of ETCO2 for IHCA. Results: A total of 207 patients were included, of which 104 (50.2%) had an IHCA and 103 (49.8%) did not. There were no differences in the mean SOFA scores at the initiation of mechanical ventilation (8.5 vs. 7.6). The ETCO2 values were decreased in the IHCA cohort, and significantly different at each time point analyzed from 300 min until immediately prior to the arrest (p < 0.001). The ETCO2 values were a mean of 20.0 mmHg in the IHCA cohort at the index time vs. 34.7 mmHg in the non-IHCA cohort (p < 0.001). The ROC analysis demonstrated moderate reliability, with an AUC = 0.687 (p < 0.0001, 95% CI 0.613–0.761) and with an ETCO2 of less than 23 mmHg, demonstrating a 67% sensitivity and a 71% specificity, as well as a 70% PPV for predicting the IHCA from our sample. Conclusions: Patients typically have rapid clinical deteriorations prior to cardiac arrest, and monitoring ETCO2 is easily achieved at the bedside while aiding in clinical decision making. The ETCO2 values in our study were significantly decreased in the IHCA cohort prior to cardiac arrest compared to the stable values in those that did not experience an IHCA, indicating that ETCO2 monitoring may have utility in predicting cardiac arrest. Further study is warranted to evaluate if predictive models utilizing ETCO2 can be constructed to predict IHCAs in mechanically ventilated ICU patients.
Angiotensin II Promotes Osteocyte RANKL Expression via AT1R Activation
Authors: Ren Jiayi , Marahleh Aseel , Ma Jinghan , Ohori Fumitoshi , Noguchi Takahiro , Fan Ziqiu , Hu Jin , Narita Kohei , Lin Angyi , Kitaura Hideki
Published: February 2025

Background/Objective: Osteocytes are the most abundant cell type in the skeleton, with key endocrine functions, particularly in regulating osteoblast and osteoclast activity to maintain bone quality. Angiotensin II (Ang II), a critical component of the renin–angiotensin–aldosterone system, is well-known for its role in vasoconstriction during hypertension. Beyond its cardiovascular functions, Ang II participates in various biological processes, including bone metabolism. While its influence on osteoblast proliferation, differentiation, and osteoclastogenesis has been documented, its effects on osteocytes remain unexplored. This study hypothesized that Ang II enhances the osteoclastogenic activity of osteocytes. Methods: Mouse calvariae were cultured ex vivo in an Ang II-containing medium, analyzed via immunohistochemistry, and evaluated for osteoclastogenic gene expression through real-time PCR. Western blotting was employed to assess protein levels and signaling pathway activation in the MLO-Y4 osteocytic cell line in vitro. Results: Ang II significantly increased the expression of receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). These effects were abrogated by azilsartan, a blocker targeting Ang II type 1 receptors (AT1R). p38 and ERK1/2 in the MAPK pathway were also activated by Ang II. Conclusions: Ang II enhances osteocyte-mediated osteoclastogenesis via AT1R activation, highlighting its potential as a therapeutic target for bone diseases.
Bone Fillers with Balance Between Biocompatibility and Antimicrobial Properties
Authors: Sorca Bogdan Valeriu , Kaya Durmuş Alpaslan , Kaya Madalina Georgiana Albu , Enachescu Marius , Ghetu Daniela-Madalina , Enache Laura-Bianca , Boerasu Iulian , Coman Alina Elena , Rusu Laura Cristina , Constantinescu Rodica , Titorencu Irina
Published: February 2025

Millions of people request bone regeneration every year, and the market for bone grafting materials has a positive trend. The most used biomaterials applied to replace and regenerate bone are based on collagen and different types of ceramics in order to mimic natural bone matrix. However, there are a lot of implant-associated infections after surgery, or the implants are rejected because of reduced biocompatibility, and this is why the research into graft bone materials is still a challenge. This study aims to develop and characterize novel biomimetic bone fillers which have simultaneously both antimicrobial properties and biocompatibility with human bone marrow—derived mesenchymal stem cells (BMSCs). Type I collagen and calcium triphosphate in a ratio of 1:1 were used as a control, according to our previous studies, and ZnO, functionalized with different percentages of Satureja thymbra L. essential oils, was added as an antimicrobial, promoting bone growth, mineralization, and formation. The bone fillers were obtained by freeze-drying in spongious forms and characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), water uptake, biodegradability over time, antimicrobial activity against Staphylococcus aureus and Escherichia coli and viability and proliferation of human BMSCs. The graft material showed a higher porosity with interconnected pores, gradual resorption over time and a balance between antimicrobial properties and biocompatibility and was chosen as an ideal bone filler.
Predictive Value of Hyperintense Acute Reperfusion Marker in Transient Ischemic Attacks: Stroke Recurrence and TOAST Classification
Authors: Han Sang-Hoon , Yum Kyu-Sun
Published: February 2025

Background: Transient ischemic attack (TIA) is a recognized precursor of stroke that also indicates a high risk of recurrence. The Hyperintense Acute Reperfusion Marker (HARM), which is linked to blood–brain barrier disruption, may serve as a novel imaging biomarker for TIA. Methods: A retrospective cohort study of 715 patients with TIA evaluated the predictive value of HARM for stroke recurrence and its association with TOAST subtype. Imaging findings, including those of diffusion-weighted imaging, were analyzed using logistic regression. Results: HARM-positive patients had significantly higher recurrence rates at 3 months (20.9%; odds ratio [OR] = 5.3) and 1 year (30.2%; OR = 5.8) compared to HARM-negative patients, relative to other imaging markers (p < 0.001). HARM was significantly associated with the cardioembolic stroke (p < 0.001). Conclusions: HARM is a promising imaging biomarker for predicting stroke recurrence and etiology in patients experiencing TIA. Incorporating HARM into clinical frameworks may enhance diagnostic and prognostic accuracy.
A Case Study on Neural Activity Characteristics in a Shooting Competition
Authors: Li Zijin , Liu Meiliang , Si Zhengye , Huang Junhao , Xu Yunfang , Zhao Zhiwen
Published: February 2025

Background: Sexual characteristics in brain neurophysiological activity are a significant area of research in cognitive neuroscience. As a sport that involves minimal physical movement, shooters remain largely stationary during aiming, facilitating the collection of their neural activity compared to athletes in other sports. Objectives: To investigate the neural characteristics of novice shooters of different genders under competitive conditions. Methods: Sixteen subjects participated in a shooting competition following four weeks of training. Electroencephalogram (EEG) data and behavioral data (shooting scores, aiming curves, and pressure curves) were recorded during the competition, and the power spectral density (PSD) and phase-locking value (PLV) network features were extracted to explore further the correlation between the shooting scores and neural activity. Results: In our sample, (1) there were no significant differences in shooting scores between males and females; (2) there were differences in PSD values across the theta, alpha, alpha-2, beta, and gamma frequency bands between males and females; and (3) there were differences in PLV network properties in the theta, alpha, beta, and gamma frequency bands between males and females. Correlation analysis revealed associations between shooting scores and neural activity in male and female novices. Conclusions: The case study demonstrated that males and females exhibited different neural activity characteristics in the shooting competition, providing a foundation for further investigation into the sex differences in neural activity in shooting competition.
Good Behavior Game: Effects on Disruptive Behaviors of Students with and Without Special Educational Needs in Inclusive Setting
Authors: Gulboy Emrah , Denizli-Gulboy Hicran , Rakap Salih
Published: February 2025

The Good Behavior Game (GBG) is a practical and evidence-based classroom management strategy that requires minimal time and resources to learn and implement. This study investigates the effectiveness of the GBG in reducing disruptive behaviors among students with special educational needs (SEN) and compares the degree of behavioral change between students with and without SEN. Additionally, the study examines students’ perceptions of the GBG. An ABAB reversal design with a follow-up phase was employed to evaluate the intervention’s effects. The study included six participants: three students with SEN who exhibited disruptive behaviors, aged 11 to 14, and three students without SEN who also exhibited disruptive behaviors, aged 11 to 13. The results indicate that the GBG is highly effective in reducing disruptive behaviors, with similar levels of improvement observed in both groups. The social validity findings highlight that students found the GBG enjoyable and engaging. The study discusses its limitations and provides practical implications for educators.
The Influence of Different Types of Moral Stories on Honest Behaviour in Children
Authors: Liang Mingyue , Wang Qianqian , Zhou Yanyan
Published: February 2025

This study aimed to explore the influence of different types of moral stories on the honest behaviour of children aged 7–11 using a field experiment. The research findings are as follows: 1. Compared to the control condition, moral stories with positive consequences of honesty significantly promote honest behaviour in children and suppress their tendency to lie. 2. The impact of different types of moral stories on honest behaviour in children varies with age. Specifically, compared to the control condition, moral stories with positive consequences of honesty significantly promote the honest behaviour of children aged 7–10. 3. Moral stories with positive consequences of honesty significantly promote honest behaviour in children because they convey positive and encouraging messages to children that “speaking the truth leads to positive consequences”. This study suggests that teachers should utilize moral stories with positive consequences of honesty more frequently to enhance students’ honest behaviour, particularly for 7–10-year-old children.
Research on the Impact of an AI Voice Assistant’s Gender and Self-Disclosure Strategies on User Self-Disclosure in Chinese Postpartum Follow-Up Phone Calls
Authors: Sun Xinxin , Shen Tianyuan , Jiang Qianling , Jiang Bin
Published: February 2025

This study examines the application of AI voice assistants in Chinese postpartum follow-up phone calls, with particular focus on how interaction design strategies influence users’ self-disclosure intention. A 2 (voice gender: female/male) × 3 (self-disclosure strategies: normal conversation without additional disclosure/objective factual disclosure/emotional and opinion-based disclosure) mixed experimental design (n = 395) was conducted to analyze how the gender and self-disclosure strategies of voice assistants affect users’ stereotypes (perceived warmth and competence), and how these stereotypes, mediated by privacy calculus dimensions (perceived risks and perceived benefits), influence self-disclosure intention. The experiment measured various indicators using a 7-point Likert scale and performed data analysis through analysis of variance (ANOVA) and structural equation modeling (SEM). The results demonstrate that female voice assistants significantly enhance users’ perceived warmth and competence, while emotional self-disclosure strategies significantly improve perceived warmth. Stereotypes about the voice assistant positively affect users’ self-disclosure intention through the mediating effects of perceived risk and benefit, with perceived benefit exerting a stronger effect than perceived risk. These findings provide valuable insights for the design and application of AI voice assistants in healthcare, offering actionable guidance for enhancing user interaction and promoting self-disclosure in medical contexts.
Promegestone Prevents Lipopolysaccharide-Induced Cervical Remodeling in Pregnant Mice
Authors: Nadeem Amna , Nadeem Lubna , Lye Stephen James , Shynlova Oksana
Published: February 2025

In most mammals, a withdrawal of the pro-gestational hormone progesterone (P4) is necessary for labor onset. In murine cervix, P4 withdrawal is mediated by enzymes steroid 5-alpha-reductase type 1 (SRD5A1) and 20-alpha-hydroxysteroid-dehydrogenase (20α-HSD). Previously, we have shown that inflammatory stimuli induce 20α-HSD levels in uterine muscle (myometrium). Here, we hypothesized that (1) infectious inflammation alters the levels of both P4-metabolizing enzymes in mouse cervix, which consequently ceases P4-mediated inhibition of cervical remodeling, thereby inducing preterm labor (PTL); (2) a progestin, selective progesterone receptor modulator promegestone (aka R5020), non-metabolizable by 20α-HSD, can block lipopolysaccharide (LPS)-induced PTL in mice by maintaining P4 signaling and preventing cervical remodeling. Using RT-PCR and IHC/IF methods, we evaluated the effect of inflammation on the expression of both enzymes in mouse cervix and determined if R5020 can prevent cervical remodeling and PTL in mice. We found significant induction of SRD5A1 and 20α-HSD proteins (p < 0.01), as well as transcript levels of pro-inflammatory cytokines Il1b, Il6, chemokines Cxcl1, Ccl2, cervical ripening enzyme Has2, hyaluronic acid binding protein/HABP (p < 0.05), and a simultaneous decrease in major extracellular fibrillar proteins, collagen type 1 and type 3 (col1a1, col3a1), in mouse cervix during PTL. The prophylactic administration of R5020 in pregnant mice significantly inhibited cervical remodeling and prevented PTL irrespective of the route of LPS-induction, systemic or local. We concluded that R5020 is a promising novel drug application for preterm birth prevention.
BCL-B Promotes Lung Cancer Invasiveness by Direct Inhibition of BOK
Authors: Ramesh Palaniappan , Al Kadi Amal R. , Borse Gaurav M. , Webendörfer Maximilian , Zaun Gregor , Metzenmacher Martin , Doerr Fabian , Bölükbas Servet , Hegedüs Balazs , Lueong Smiths S. , Magne Joelle , Liu Beiyun , Nunez Greisly , Schuler Martin , Green Douglas R. , Kalkavan Halime
Published: February 2025

Expression of BCL-B, an anti-apoptotic BCL-2 family member, is correlated with worse survival in lung adenocarcinomas. Here, we show that BCL-B can mitigate cell death initiation through interaction with the effector protein BOK. We found that this interaction can promote sublethal mitochondrial outer membrane permeabilization (MOMP) and consequently generate apoptosis-flatliners, which represent a source of drug-tolerant persister cells (DTPs). The engagement of endothelial-mesenchymal-transition (EMT) further promotes cancer cell invasiveness in such DTPs. Our results reveal that BCL-B fosters cancer cell aggressiveness by counteracting complete MOMP.
Oversecretion of CCL3 by Irradiation-Induced Senescent Osteocytes Mediates Bone Homeostasis Imbalance
Authors: Zhao Fanyu , Han Haiqing , Wang Jing , Wang Jianping , Zhai Jianglong , Zhu Guoying
Published: February 2025

Various stressors such as ionizing radiation (IR), chemotherapeutic agents, oxidative stress, and inflammatory responses can trigger the stress-induced premature senescence (SIPS) of cells in the bone microenvironment, including osteocytes. However, little is known about the mechanisms underlying the senescent cellular regulation of the differentiation potential and bone homeostasis. Here, we report a secretory change in senescent osteocytes activated by IR, its subsequent impact on osteogenic and osteoclastic differentiation, and the inflammatory cascade response. It was observed that osteocytes exhibited altered biological function, persistent and incomplete DNA damage repair, and characteristic senescence phenotypes after exposure to IR in vitro. Meanwhile, a concomitant increase in the CC chemokine ligand 3 (CCL3), a key component of the senescence-associated secretory phenotype (SASP), was observed in the IR-induced senescent osteocytes, which could further downregulate the osteogenic differentiation and enhance the osteoclastic differentiation in cell supernatant co-culture experiments. Notably, the enhancement of the PI3K/Akt/NF-κB signaling pathway in IR-induced senescent osteocytes appears to be an essential driver of the imbalance between the osteogenic and osteoclastic differentiation potentials. Taken together, these data suggest a novel role of CCL3 in IR-induced bone homeostatic imbalance through SASP cascade secretion, mediated by the PI3K/Akt/NF-κB signaling pathway.
Immunomodulatory Tissue Factors in the Gallbladder Walls of Pediatric Patients with Chronic Calculous Cholecystitis
Authors: Zariņa Kaiva Zīle , Pilmane Māra , Pētersons Aigars
Published: February 2025

Background: The rising rates of gallstones and cholecystectomy in pediatric populations underscore the increasing concern regarding chronic cholecystitis. However, the morphopathogenesis of pediatric calculous cholecystitis is still not well understood. This study aimed to determine the expression and distribution of immunomodulatory factors interleukin-12 (IL-12), interleukin-13 (IL-13), interleukin-1β (IL-1β), sonic hedgehog protein (SHH), nuclear factor NF-kappa-B p65 subunit (NFkBp65), and heat shock protein 60 (HSP60) in the gallbladder walls of pediatric patients with chronic calculous cholecystitis. Methods: In total, 11 gallbladder samples were collected from pediatric patients with calculous cholecystitis during cholecystectomy, while 5 healthy gallbladder samples served as controls. IL-12, IL-13, IL-1β, SHH, NFkBp65, and HSP60 were detected by immunohistochemistry. The number of positive structures in gallbladder wall epithelium, vasculature, and inflammatory infiltrate was assessed semi-quantitatively by microscopy. A Mann–Whitney U test and Spearman’s rank-order correlation coefficient were calculated. Results: Statistically significant differences were observed between patient and control samples in the expression of IL-1β, SHH, and NFkBp65 in the epithelium, as well as in the expression of IL-12, SHH, and HSP60 in the blood vessels. The expression of IL-1β was stronger in the epithelium of controls, while other markers were more prominent in patient samples. Conclusions: An increased number of NFkBp65, IL-12, and HSP60 positive cells in patient gallbladder tissue suggests a significant role of these tissue factors in driving immune modulation and sustaining the inflammation in pediatric chronic calculous cholecystitis. The noticeable expression of SHH in patient gallbladder tissue indicates its part in tissue regeneration and repair processes, as well as in modulating inflammation and vascular responses in calculous cholecystitis. The significant positive correlations between the factors studied highlight the importance of their coordinated interaction and intricate crosstalk in the morphopathogenesis of calculous cholecystitis.
Integrating Kata Training into School Education: Effects on Sustained Attention and Cognitive Performance in 8–9-Year-Old Children
Authors: Gökdere Fuat , Uylas Erdem , Çatıkkaş Fatih , Günay Erkan , Ceylan Halil İbrahim , Özgören Murat
Published: February 2025

(1) Background: The ability to sustain attention in primary school children aged 8–9 years plays a critical role in maintaining focus for extended periods, enabling them to comprehend and integrate large amounts of information. Enhancing sustained attention during this formative stage significantly improves a child’s capacity to acquire and consolidate new skills and knowledge, laying a strong foundation for academic and cognitive development. (2) Objectives: This study aimed to assess the effect of an 8-week kata training program on attention and its components in 8–9-year-old school-age children, a critical developmental period for attention. (3) Methods: After excluding the participants who gave a low number of correct answers in the pre-test or created a ceiling effect, 43 participants, aged 9.12 ± 0.40 years, were included in this study. This study, conducted during the academic term, included three measurement phases and a familiarization session. Attention parameters were evaluated using the Bourdon–Vos Test, and participants were categorized into Low Performers (LP) and High Performers (HP) based on pre-test scores. The intervention group (INT) underwent kata training thrice weekly for 8 weeks, while the control group (CON) followed their regular activities. Post-training, attention parameters were reassessed using the Bourdon–Vos Test. (4) Results: In the post-intervention analysis, significant improvements in the number of correct responses were observed in both the LP (p < 0.001, Cohen’s d = −1.333) and HP (p = 0.001, Cohen’s d = −1.644) groups within the INT group. In the CON group, significant improvement was observed only in the HP group (p = 0.031, Cohen’s d = −0.948). Regarding attention processing speed, significant pre-post improvements were found exclusively in the INT group (p < 0.001). Block-wise analysis revealed significant differences only in Block 1 of the CON group (p = 0.011, Cohen’s d = −0.522). However, in the INT group, significant improvements were observed in both Block 1 (p < 0.001, Cohen’s d = −1.200) and Block 2 (p = 0.004, Cohen’s d = −0.678). (5) Conclusions: The findings of this study highlight the effectiveness of an 8-week kata training program in enhancing sustained attention and cognitive processing speed among 8–9-year-old children, particularly in low-performing groups. This suggests that integrating structured kata-based motor and cognitive activities into school curricula can serve as a promising strategy for addressing attention deficits and promoting cognitive development during this critical developmental period. Future studies should examine the long-term effects of kata training on attention and related cognitive functions, such as working memory and executive control. Investigating neurophysiological mechanisms through neuroimaging and including diverse age groups with larger samples could further validate these findings.
Off-Grid Methodology for Sustainable Electricity in Medium-Sized Settlements: The Case of Nisyros Island
Authors: Tsiaras Evangelos , Andreosatou Zografia , Kouveli Aliki , Tampekis Stergios , Coutelieris Frank A.
Published: February 2025

As a crucial strategy for mitigating climate change and achieving electricity independence, renewable energy sources (RESs) are gaining widespread importance. This study explores achieving electricity autonomy for Nisyros Island, Greece, through RESs. Four scenarios are evaluated, including standalone wind and photovoltaic systems, alongside hybrid options combining both. Each scenario is designed to meet the island’s electricity demands while considering economic feasibility and minimal environmental impact. The research findings are that wind-based scenarios offer the most cost-effective solutions, with a three wind turbine setup emerging as the most economical option for full coverage of electricity demands. Hybrid approaches, particularly those incorporating more wind turbines, are also financially viable. Real-world consumption data are integrated into the analysis, providing valuable insights for Nisyros’ energy future. Overall, the study demonstrates Nisyros’ potential to achieve electricity independence through RESs, with wind resource assessments suggesting that the island could become autonomous. This approach would promote environmental sustainability by reducing the given dependence on fossil fuels. Additionally, it would bring economic benefits for the island’s residents in the renewable energy sector. Furthermore, this work allows for the island to achieve electricity independence through renewable energy in alignment with the EU’s climate goals.