bluuebunny/crossref_metadata_2025 huggingface dataset:
PSIV-B-20 A fecal arsenic excretion pattern in pigs fed arsenic-containing rice bran
Authors: Liao Shengfa F , Hasan Shamimul , Stevens Andrew W , Yang Zhongyue , Feugang Jean M , Peng Zhaohua
Published: October 2021

Rice bran is a common feed/food stuff for animals and humans; however, it usually contains a substantial amount of arsenic that is a class-I, non-threshold carcinogen to humans. This study was to investigate the fecal arsenic excretion pattern from the pigs fed an arsenic-containing rice bran. Twenty growing gilts (26.3 kg) were randomly assigned to 3 dietary treatments (n = 6 or 7) with Diets I, II, and III containing 0, 36.7, and 73.5% rice bran, and 0, 306, and 612 ppb arsenic, respectively. The feeding trial lasted 6 weeks, and fresh fecal samples were collected on Days 0, 14, 28, and 42 post-treatments. Fecal arsenic concentrations were analyzed using an ICP-MS protocol standardized by the U.S. Environmental Protection Agency. Tobit statistical model was employed for data analysis because some censored values were obtained from the laboratory sample analysis. Results (Table 1) showed that there were no differences in fecal arsenic concentrations from Day 0 through 42 post-treatment in pigs fed Diet I (a control diet). Nevertheless, the fecal arsenic concentration was increased significantly (P < 0.05) on Days 14 and 28 when compared to Day 0 post-treatment with Diet II (a moderate level of rice bran inclusion), and it was further increased (P < 0.05) on Day 42 post-treatment. The fecal arsenic excretion pattern in pigs fed Diet III (a high level of rice bran inclusion) was the same as that in pigs fed Diet II. The dietary treatment effects on fecal arsenic excretion were already reported previously. In short, the results of this study suggest that the fecal arsenic excretion is increased in the first 4 weeks and then further increased around the 6th week since the start of feeding arsenic-containing rice bran. This further increase may be due to the saturation of arsenic retention in pig body.
PSIV-B-19 Effects of root extract from Achyranthes japonica Nakai on the growth performance, blood profile, fecal microbial community, fecal gas emission, and meat quality of finishing pigs
Authors: Deokho Koo , De Xin Dang , Hoque Raihanul , Bin Lim Chain , Inho Kim
Published: October 2021

This study investigated the effects of a root extract from Achyranthes Japonica Nikai (AJN) on the growth performance, blood profile, fecal microbial community, fecal gas emission, and meat quality of finishing pigs. A total of 72 crossbreed pigs [(Landrace × Yorkshire) × Duroc], with an initial body weight of 50.28 ± 3.77 kg were used in a 70-day experiment in two phases: phase 1 (days 0–35) and phase 2 (days 36–70). Dietary treatments consisted of feeding pigs; 1) CON, corn-soybean-wheat basal diet; 2) TRT1, CON + 0.1% AJN root extract; and 3) TRT2, CON + 0.2% AJN root extract. There were six replicate pens per treatment and four pigs (two barrows and two gilts) per pen. The replicate pen was used as the experimental unit. Orthogonal contrasts were used to examine the linear and quadratic effects in response to increasing the dietary supplementation of AJN root extract. Average daily gain (ADG) during phase 2 (P = 0.01) increased linearly with the dose of AJN root extract, while drip loss on day 3 (P = 0.04) and ammonia emission during phase 2 (P = 0.01) decreased linearly. However, the blood profile and fecal microbial community did not differ among the diet groups. In conclusion, AJN root extract increased ADG during days 36–70 and reduced drip loss from meat samples. It also reduced fecal ammonia emission, which improve the overall barn environment.
PSIV-B-21 Broilers as an animal model to assess different copper, manganese and zinc mineral sources on absorption
Authors: Boyer Angela R , Roberts Heaven , Nuzback Dennis , Garcia Miriam
Published: October 2021

The study objective was to evaluate absorption differences between different sources of Copper (Cu), Manganese (Mn) and Zin (Zn) in broiler diets. Cobb500 chicks (n = 130, 0-d old) were housed in colony brooders and offered basal diet and water ad libitum for eight days. Following the transition phase, 96 straight-run chicks were randomly assigned to one of 48 battery cages (2 chicks/cage; 12 cages/treatment) and offered one of three soy-corn based diets for three weeks: Negative Control (NC; Basal diet at 6 mg/kg Cu, 35 mg/kg Mn, and 36 mg/kg Zn); Positive Control (PC; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from Sulfate sources); Treatment 1 (T1; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from hydroxychloride Product 1); and Treatment 2 (T2; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from hydroxychloride sources Product 2). Week three birds were bled and dissected to obtain liver, breast and femur samples for mineral absorption status. Data were analyzed using RV3.6.1 procedures with non-repeated measures. Plasma and breast tissue were not (P ≥ 0.05) impacted by mineral source, however numerical increases were reported in PC, T1 and T2 over NC. Liver Cu and Zn concentrations were non-significant (P ≥ 0.05) for all treatments. However liver Mn concentrations increased (P ≤ 0.05) in Pc and T1. Copper bone concentrations were not (P ≥ 0.05) affected, but an increase (P ≤ 0.05) in Mn and Zn was reported for PC, T1 and T2 versus NC. These results indicate increased absorption occurs when mineral sources are adequately supplemented beyond basal diet levels and that source may impact absorption rate. Additional research is needed to address the absorption ability of different mineral sources to better understand the impact on bird performance.
PSIV-B-25 Digestible energy and metabolizable energy of high-fiber ingredients in growing pig diets
Authors: Kim Jongkeon , Jo Yun Yeong , Kim Beob Gyun G
Published: October 2021

The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P < 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P < 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.
PSIV-B-22 Comparative effects of nanoparticle-selenium, selenium-enriched yeast and sodium selenite antagonize cadmium-induced cardiotoxicity in chicken (Gallus gallus) via modulating AHR/CAR/PXR/Nrf2 pathways
Authors: Ge Jing , Huang Yan , Li Jinlong
Published: October 2021

Selenium (Se), a nutritionally essential mineral for human and animals, has a significant antagonistic effect on heavy metal cadmium (Cd) biotoxicity. Still, the impact of different Se source on alleviating Cd toxicity has received only limited attention. Therefore, the purpose of the current study was to assess the mitigation level of Cd-induced cardiotoxicity by different sources such as nanoparticles of selenium (Nano-Se), selenium-rich yeast (SY) and sodium selenite (SS). Two hundred of male, 1-day old Hy-Line Variety White chickens were randomly, equally divided into five groups (n = 25). These chicken groups were assigned to (1) basal diet (Con), (2) 140 mg/kg CdCl[2] of basal diet Cd (Cd), (3) 1 mg/kg Nano-Se plus 140 mg/kg CdCl[2] of basal diet (Nano-Se + Cd), (4) 3 mg/kg selenium-enriched yeast plus 140 mg/kg CdCl[2] of basal diet (SY + Cd group), and (5) 3 mg/kg sodium selenite plus 140 mg/kg CdCl[2] of basal diet (SS+ Cd group). The results evidenced that presence of Cd led to a significant increase in biochemical parameters such as lactate dehydrogenase (LDH) and creatine kinase (CK), as well as histopathological lesions in the heart of chickens. Cd exposure also resulted in more extensive effects on phase I metabolism enzymes and transcript CYP isoforms, elevated the levels of MDA and H[2]O[2] and depressed total superoxide dismutase (T-SOD), Cu-Zn SOD, total antioxidant capacity (T-AOC) and catalase (CAT) activities. The expression of nuclear receptors (NRs), AHR, CAR and PXR was declined, down-regulated Nrf2 and its downstream targets in the Cd-treat group. Notably, Se sources application alleviated Cd toxicity by triggering AHR/CAR/PXR/Nrf2 signaling pathway to promote restoring antioxidant defense system and phase I metabolism enzymes system. However, when compared the effectiveness of antagonism, the Nano-Se was superior in relieving Cd-induced cardiotoxicity via AHR/CAR/PXR/Nrf2 pathway activation than other Se- sources.
PSVI-3 Effect of feeding resistant starch on sow and piglet farrowing performance and blood parameters
Authors: Markland Mary L , Lay Donald C , Richert Brian T
Published: October 2021

Hyperprolific sows can have a long duration of farrowing, negatively impacting piglet survival and vitality. This study’s objective was to assess the effect of a diet containing 6.6% resistant starch (RS) on sow and piglet farrowing performance. We hypothesized that a diet containing RS would decrease sow farrowing duration and improve piglet survival. Forty-two sows were balanced for parity and randomly assigned to 1 of 2 treatments: standard lactation diet (n = 21) or diet containing RS (n = 21). Sows were fed their respective diets from approximately 7d prefarrowing throughout lactation. Sow blood was drawn at three time points on d106±1.0 and d113±1.0 of gestation (pre-feeding, 2 and 6 h post-feeding) and during farrowing to measure blood glucose. Piglet umbilical blood (~3/litter; beginning, middle, end of birth order) was collected at birth and analyzed for glucose, lactate, pH, PCO2, PO2, TCO2, HCO3, BE, and sO2. Piglets were visually assessed at birth to score meconium staining. Data collected included farrowing duration, piglet birthing intervals, number of stillborn piglets/litter, sow and piglet weights, piglet mortality, and sow feed and water intake. Average litter sizes post-crossfoster for control and RS were similar, 12.1±0.4 and 11.6±0.4 piglets, respectively. Litter weight gain was similar between control and RS (44.97 vs. 47.54 kg, P = 0.33), respectively. Feeding resistant starch had no effect on number of stillborn piglets or pre-weaning survival (P > 0.75). Farrowing duration (178.9±17.6 min vs 165.2±18.0 min, P = 0.59) and piglet birth intervals (16.57±1.7 min and 14.89±1.8 min, P = 0.51) did not differ between control and RS, respectively. There was difference in sow blood glucose between diets (P = 0.02), day of sample (P < 0.0001), time point (P < 0.0001), and a day by time point interaction (P < 0.0001). Feeding RS to sows prefarrowing can modify sow blood glucose, but did not result in reduced farrowing duration or improved piglet survival.
Long non-coding RNA AFAP1-AS1 promotes thyroid cancer progression by sponging miR-204-3p and upregulating DUSP4
Authors: Ma Hongzhi , Shi Qian , Fang Jugao , Wang Ru , Zhao Jianyu , Lin Sitong , Dong Jiajing , Zhang Yan , Shen Xixi , Chen Jiaming , Zhong Qi
Published: October 2021

Long non-coding RNA actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) shows crucial regulatory function in tumor progression. Nonetheless, the biological function and underlying mechanism of AFAP1-AS1 in the progression of thyroid cancer is still unclear. Expressions of AFAP1-AS1, miR-204-3p and DUSP4 were quantified utilizing quantitative real-time polymerase chain reaction and/or western blot. In loss-of-function and gain-of-function assays, cell proliferation, migration and invasion were appraised by CCK-8 assay, wound healing assay, Transwell migration and invasion assays, respectively. Luciferase reporter assay was employed for validating the interaction between miR-204-3p and AFAP1-AS1 or the 3’UTR of dual specificity phosphatase 4 (DUSP4). AFAP1-AS1 was highly expressed in thyroid cancer tissues and cell lines. Highly expressed AFAP1-AS1 was in association with advanced TNM stage and positive lymph node metastasis. Knockdown of AFAP1-AS1 suppressed the proliferation, migration and invasion of thyroid cancer cells, and overexpression of AFAP1-AS1 induced a reversed effect. MiR-204-3p was targetedly repressed by AFAP1-AS1, and miR-204-3p could negatively regulate DUSP4 expression. AFAP1-AS1 augmented the expression of DUSP4 via repressing miR-204-3p, and the effects of AFAP1-AS1 overexpression on thyroid cancer cells were also partly abolished by miR-204-3p restoration. In summary, AFAP1-AS1 facilitates thyroid cancer cell proliferation, migration and invasion by regulating miR-204-3p/DUSP4 axis.
Forty years of the structure of plant-type ferredoxin
Authors: Kurisu Genji , Tsukihara Tomitake
Published: October 2021

The X-ray structure of a [2Fe-2S]-type ferredoxin (Fd) from Spirulina platensis, solved by a collaborative group led by Profs Masao Kakudo, Yukiteru Katsube and Hiroshi Matsubara, was the first high-resolution structure of a plant-type Fd deposited in the Protein Data Bank. The main chain structure, comprising a [2Fe-2S] cluster ligated by four conserved cysteine residues, together with a molecular evolutionary study based on a series of amino acid sequence determinations, was reported in Nature in 1980. The consequent detailed crystallographic analysis, including crystallization, heavy atom derivatization, data collection, phase calculation and model building, was published by the same group in the Journal of Biochemistry in 1981. The pioneering X-ray analysis of S. platensis Fd at 2.5 Å resolution was a key milestone in structural research on the photosynthetic electron transport chain, informing related and challenging studies on other components of the photosynthetic electron transfer chain.
A Synthesis of Game Theory and Quantitative Genetic Models of Social Evolution
Authors: McGlothlin Joel W , Akçay Erol , Brodie Edmund D , Moore Allen J , Van Cleve Jeremy
Published: October 2021

Two popular approaches for modeling social evolution, evolutionary game theory and quantitative genetics, ask complementary questions but are rarely integrated. Game theory focuses on evolutionary outcomes, with models solving for evolutionarily stable equilibria, whereas quantitative genetics provides insight into evolutionary processes, with models predicting short-term responses to selection. Here we draw parallels between evolutionary game theory and interacting phenotypes theory, which is a quantitative genetic framework for understanding social evolution. First, we show how any evolutionary game may be translated into two quantitative genetic selection gradients, nonsocial and social selection, which may be used to predict evolutionary change from a single round of the game. We show that synergistic fitness effects may alter predicted selection gradients, causing changes in magnitude and sign as the population mean evolves. Second, we show how evolutionary games involving plastic behavioral responses to partners can be modeled using indirect genetic effects, which describe how trait expression changes in response to genes in the social environment. We demonstrate that repeated social interactions in models of reciprocity generate indirect effects and conversely, that estimates of parameters from indirect genetic effect models may be used to predict the evolution of reciprocity. We argue that a pluralistic view incorporating both theoretical approaches will benefit empiricists and theorists studying social evolution. We advocate the measurement of social selection and indirect genetic effects in natural populations to test the predictions from game theory and, in turn, the use of game theory models to aid in the interpretation of quantitative genetic estimates.
Population Dynamics of Wolbachia in the Leafhopper Vector Yamatotettix flavovittatus (Hemiptera: Cicadellidae)
Authors: Wangkeeree Jureemart , Sanit Piyatida , Roddee Jariya , Hanboonsong Yupa
Published: October 2021

Wolbachia (Rickettsiales: Alphaproteobacteria) infections induce abnormalities in the reproductive system and affect various biological traits of the host insects. The density of Wolbachia is one of the major parameters that influence induced phenotypes and interactions with the hosts. Wolbachia occurs naturally in populations of the leafhopper Yamatotettix flavovittatus Matsumura (Hemiptera: Cicadellidae), which transmits phytoplasma that cause white leaf disease in sugarcane. However, the quantity and dynamics of Wolbachia in this leafhopper are not well understood. In the current study, we estimated the number of Wolbachia by absolute quantification of the copy number of wsp, which encodes the outer surface protein, using real-time quantitative polymerase chain reaction (PCR). This investigation was performed using natural populations and laboratory colonies from three lineages of leafhoppers (designated as UD, KK, and SK). There was no significant difference in the number of wsp copies in most of field-collected adults. During the immature developmental stages, there were differences in the dynamics of Wolbachia infection between the UD lineage and the other two lineages. However, the number of wsp copies increased in the early instar and plateaued in the later nymphal instars. Sex had no influence on the number of Wolbachia within the same lineages. The number of Wolbachia was relatively constant during the adult stage in the UD lineage but fluctuated in the other two lineages. In conclusion, the present data provide a framework for exploring the relationship between Wolbachia and the leafhopper and could facilitate future research into management strategies using Wolbachia.
cGAS‒STING signaling and function in metabolism and kidney diseases
Authors: Bai Juli , Liu Feng
Published: October 2021

The cyclic GMP‒AMP synthase (cGAS)‒stimulator of interferon genes (STING) signaling pathway senses the presence of cytosolic DNA and, in turn, triggers downstream signaling to induce the expression of inflammatory and type I interferon genes in immune cells. Whereas the innate immune function of the cGAS‒STING pathway is well studied over the past years, emerging evidence suggests that this signaling pathway may have additional functions beyond innate immune surveillance. Consistent with this notion, dysregulation of the cGAS‒STING signaling pathway in adipocytes, hepatocytes, and renal proximal tubule epithelial cells are associated with metabolic dysfunction, impaired energy homeostasis, and kidney diseases. In this review, we summarize current understanding of the cGAS‒STING pathway in several metabolic diseases such as obesity, insulin resistance, alcoholic and nonalcoholic fatty liver diseases, as well as acute kidney injury and chronic kidney disease. We also review the interaction between the cGAS‒STING pathway and lipid metabolism. Lastly, we discuss potential mechanisms by which cGAS‒STING signaling regulates metabolism and point toward future avenues of research targeting the cGAS‒STING pathway as possible means to treat common metabolic disorders.
Who Gets Referred? A Pilot Study of Risk Stratification and Treatment Referral in Pediatric Headache Using the Pediatric Pain Screening Tool
Authors: Salamon Katherine S , Carlson Megan , Hildenbrand Aimee K
Published: October 2021

Objective

Headaches are common among youth and are associated with significant negative outcomes. Despite advances in interdisciplinary treatments for youth with chronic pain, research suggests disparities in access to these services.

Methods

A total of 186 youth (M = 14.19 years old, 70.8% female) presenting to a neurology appointment at a children’s hospital system were screened using the Pediatric Pain Screening Tool (PPST), a brief, validated measure to identify youth that may benefit from additional pain management services.

Results

Two-thirds of participants (n = 124, 66.7%) screened as medium or high risk on the PPST. Risk categorization did not vary by patient age or sex. A greater proportion of Hispanic/Latino patients were categorized as low-risk relative to non-Hispanic/Latino patients (55.6% vs. 30.1%), and a somewhat lower proportion of patients of color were categorized as medium-risk relative to White patients (14.0% vs. 30.5%). Three-quarters (n = 94, 75.8%) of patients who were screened as medium or high risk were not referred for any additional pain management services. Referrals did not vary by patient age or ethnicity. While not statistically significant, a lower proportion of males received referrals at both medium (8.3% vs. 17.6%) and high levels of risk (15.8% vs. 34.5%), and a greater proportion of youth of color who screened as medium risk received referrals relative to White youth categorized as medium risk (37.5% vs. 10.3%).

Conclusion

Future research should continue to explore factors influencing decision-making regarding referral to specialized pain management services for youth with headache.
Remifentanil protects heart from myocardial ischaemia/reperfusion (I/R) injury via miR-206-3p/TLR4/NF-κB signalling axis
Authors: Zhang Dongyun , Wang Qun , Qiu Xunbin , Chen Yiguan , Yang Xiaoli , Guan Yujian
Published: October 2021

Objectives

Myocardial I/R injury is one of the most serious complications after reperfusion therapy in patients with myocardial infarction. Remifentanil has been found to protect the heart against I/R injury. However, its underlying mechanism remains uncertain in myocardial I/R injury.

Methods

The myocardial I/R injury rat model was established by 30 min of ischaemia followed by 24 h of reperfusion. The animal model was evaluated by the levels of TC, ALT and AST and H&E staining. The binding of miR-206-3p and TLR4 was predicted and verified using TargetScan software, luciferase reporter and RNA pull-down assays. The functional role and mechanism of remifentanil were identified by ultrasonic echocardiography, oxidative stress markers, H&E, Masson and TUNEL staining and western blot.

Key findings

The rat myocardial I/R injury model displayed a significantly high level of TC, ALT, AST, TLR4, p-IκBα and p-p65 and the presence of disorganized cells and inflammatory cell infiltration. The model also showed increased levels of LVEDD, LVESD, MDA, fibrosis and apoptosis and decreased levels of EF, FS, SOD and GSH, which were reversed with remifentanil treatment. Knockdown of miR-206-3p damaged cardiac function and aggravated oxidative stress. miR-206-3p could directly bind to TLR4. TLR4 overexpression destroyed cardiac function, exacerbated oxidative stress, increased levels of p-IκBα and p-p65 and aggravated pathology manifestation affected by remifentanil.

Conclusions

Our results elucidated that remifentanil alleviated myocardial I/R injury by miR-206-3p/TLR4/NF-κB signalling axis.
SABR pre-treatment checks using alanine and nanoDot dosimeters
Authors: Esen Nsikan , Ramachandran Prabhakar , Geso Moshi
Published: October 2021

Stereotactic Ablative Radiotherapy (SABR) remains one of the preferred treatment techniques for early-stage cancer. It can be extended to more treatment locales involving the sternum, scapula and spine. This work investigates SABR checks using Alanine and nanoDot dosimeter for three treatment sites, including sternum, spine and scapula. Alanine and nanoDot dosimeters’ performances were verified using a 6 MV photon beam before SABR pretreatment verifications. Each dosimeter was placed inside customized designed inserts into a Rod Phantom (in-house phantom) made of Perspex that mimics the human body for a SABR check. Electron Paramagnetic Resonance (EPR) spectrometer, Bruker EleXsys E500 (9.5 GHz) and Microstar (Landauer Inc.) Reader was employed to acquire the irradiated alanine and nanoDot dosimeters’ signal, respectively. Both dosimeters treatment sites are expressed as mean ± standard deviation (SD) of the measured and Eclipse calculated dose Alanine (19.59 ± 0.24, 17.98 ± 0.15, 17.95 ± 0.18) and nanoDot (19.70 ± 0.43, 17.05 ± 0.08, 17.95 ± 0.98) for spine, scapula and sternum, respectively. The percentage difference between alanine and nanoDot dosimeters was within 2% for sternum and scapula but 2.4% for spine cases. These results demonstrate Alanine and nanoDot dosimeters’ potential usefulness for SABR pretreatment quality assurance (QA).
A high attack rate of 90% of SARS-CoV-2 Delta variant infections in crew personnel on a single navy ship
Authors: Lee Byung Uk
Published: October 2021

A total of 272 soldiers out of the 301 soldiers (90.4%) were infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant of concern (VOC) on a single navy ship. This outbreak provides three lessons for the pandemic. This incident clearly demonstrates the transmission characteristics of the SARS-CoV-2 Delta VOC.
Storage Duration and Red Blood Cell–Derived Microparticles in Packed Red Blood Cells Obtained from Donors with Thalassemia
Authors: Noulsri Egarit , Lerdwana Surada , Palasuwan Duangdao , Palasuwan Attakorn
Published: October 2021

Objective

To address the effects of storage duration on red blood cell (RBC)–derived microparticles (RMPs) in packed RBCs from donors who have thalassemia.

Materials and Methods

Packed RBCs were prepared according to laboratory routine. The quantity of RMPs was determined using FACSCalibur and counting beads.

Results

Across durations of storage, the packed RBCs from donors with thalassemia (n = 28) and healthy volunteers (n = 104) showed average RMPs to be 47,426 (10,139‒127,785) particles/μL vs 49,021 (13,033‒126,749) particles/μL, respectively (P = .63). The peak RMP levels in donors with thalassemia and healthy volunteers, respectively, were shown in products from storage days 34 and 38. Both groups showed a trend toward a positive association between RMP concentration and the duration of storage in packed RBC bags stored under blood bank conditions.

Conclusion

Our results suggest that storage-induced RMP release has similar effects in stored packed RBCs obtained from both donors with thalassemia and healthy volunteers.
Numerical experiments on dynamic evolution of a CME-flare current sheet
Authors: Xie Xiaoyan , Mei Zhixing , Shen Chengcai , Cai Qiangwei , Ye Jing , Reeves Katharine K , Roussev Ilia I , Lin Jun
Published: October 2021

In this paper, we performed magnetohydrodynamics numerical experiments to look into the dynamic behaviour of the current sheet (CS) between the coronal mass ejection (CME) and the associated solar flare, especially the CS oscillation and plasmoid motions in coronal conditions. During the evolution, the disrupting magnetic configuration becomes asymmetric first in the buffer region at the bottom of the CME bubble. The Rayleigh−Taylor instability in the buffer region and the deflected motion of the plasma driven by the termination shock at the bottom of the CME bubble cause the buffer region to oscillate around the y-axis. The local oscillation propagates downwards through the CS, prompting an overall CS oscillation. As the buffer region grows, the oscillation period becomes longer, increasing from about 30 s to about 16 min. Meanwhile, there is another separated oscillation with a period between 0.25 and 1.5 min in the cusp region of the flare generated by velocity shearing. The tearing mode instability yields formations of plasmoids inside the CS. The motions of all the plasmoids observed in the experiment accelerate, which implies that the large-scale CME/flare CS itself in the true eruptive event is filled with the diffusion region according the the standard theory of magnetic reconnection.
Comparing the pre-SNe feedback and environmental pressures for 6000 H iiregions across 19 nearby spiral galaxies
Authors: Barnes A T , Glover S C O , Kreckel K , Ostriker E C , Bigiel F , Belfiore F , Bešlić I , Blanc G A , Chevance M , Dale D A , Egorov O , Eibensteiner C , Emsellem E , Grasha K , Groves B A , Klessen R S , Kruijssen J M D , Leroy A K , Longmore S N , Lopez L , McElroy R , Meidt S E , Murphy E J , Rosolowsky E , Saito T , Santoro F , Schinnerer E , Schruba A , Sun J , Watkins E J , Williams T G
Published: October 2021

The feedback from young stars (i.e. pre-supernova) is thought to play a crucial role in molecular cloud destruction. In this paper, we assess the feedback mechanisms acting within a sample of 5810 H ii regions identified from the PHANGS-MUSE survey of 19 nearby (<20 Mpc) star-forming, main-sequence spiral galaxies [log(M⋆/M⊙) = 9.4–11]. These optical spectroscopic maps are essential to constrain the physical properties of the H ii regions, which we use to investigate their internal pressure terms. We estimate the photoionized gas (Ptherm), direct radiation (Prad), and mechanical wind pressure (Pwind), which we compare to the confining pressure of their host environment (Pde). The H ii regions remain unresolved within our ∼50–100 pc resolution observations, so we place upper (Pmax) and lower (Pmin) limits on each of the pressures by using a minimum (i.e. clumpy structure) and maximum (i.e. smooth structure) size, respectively. We find that the Pmax measurements are broadly similar, and for Pmin the Ptherm is mildly dominant. We find that the majority of H ii regions are overpressured, Ptot/Pde = (Ptherm + Pwind + Prad)/Pde > 1, and expanding, yet there is a small sample of compact H ii regions with Ptot,max/Pde < 1 (∼1 per cent of the sample). These mostly reside in galaxy centres (Rgal < 1 kpc), or, specifically, environments of high gas surface density; log(Σgas/M⊙ pc−2) ∼ 2.5 (measured on kpc-scales). Lastly, we compare to a sample of literature measurements for Ptherm and Prad to investigate how dominant pressure term transitions over around 5 dex in spatial dynamic range and 10 dex in pressure.
Spiral density-wave structure parameters in the solar neighbourhood derived from longitudinal velocities of Gaia EDR3 OB stars: 3D approach
Authors: Griv Evgeny , Gedalin Michael , Mróz Przemek , Liu De-Jian , Jiang Ing-Guey
Published: October 2021

Distances and transverse along the Galactic longitude velocities vl of 2640 Gaia EDR3 O–B2 stars of Xu et al. within 2.5 kpc from the Sun and 250 pc from the Galactic plane with relative distance and velocity accuracies of <10 and <50 per cent are selected. Under the assumption of spiral density waves, both Galactic differential rotation parameters and parameters of the wave structure in this solar neighbourhood are derived from observed vl. In contrast to all preceding studies, we take into account the effect of small but finite thickness of the disc of the Galaxy on even parity (‘sausage’) gravity perturbations of the kind investigated by Lin and Shu. As previously predicted by the modified theory of 3D density waves, two scales of periodic rarefaction–compression irregularity of the vl velocity field with the radial λ0 ≈ 1.5 kpc and vertical ξ0 ≈ 1.0 kpc wavelengths in the form of a spiral wave propagating in the disc are revealed. The Gaia’s DR2 line-of-sight and EDR3 longitudinal velocities analyses performed in the last papers of a series exhibit consistent findings, thus providing a further evidence to support the Lin–Shu density-wave proposal.
compaso: A new halo finder for competitive assignment to spherical overdensities
Authors: Hadzhiyska Boryana , Eisenstein Daniel , Bose Sownak , Garrison Lehman H , Maksimova Nina
Published: October 2021

We describe a new method (compaso) for identifying groups of particles in cosmological N-body simulations. compaso builds upon existing spherical overdensity (SO) algorithms by taking into consideration the tidal radius around a smaller halo before competitively assigning halo membership to the particles. In this way, the compaso finder allows for more effective deblending of haloes in close proximity as well as the formation of new haloes on the outskirts of larger ones. This halo-finding algorithm is used in the abacussummit suite of N-body simulations, designed to meet the cosmological simulation requirements of the Dark Energy Spectroscopic Instrument (DESI) survey. compaso is developed as a highly efficient on-the-fly group finder, which is crucial for enabling good load-balancing between the GPU and CPU and the creation of high-resolution merger trees. In this paper, we describe the halo-finding procedure and its particular implementation in abacus, accompanying it with a qualitative analysis of the finder. We test the robustness of the compaso catalogues before and after applying the cleaning method described in an accompanying paper and demonstrate its effectiveness by comparing it with other validation techniques. We then visualize the haloes and their density profiles, finding that they are well fit by the NFW formalism. Finally, we compare other properties such as radius–mass relationships and two-point correlation functions with that of another widely used halo finder, rockstar.
Are interactions with neutron star merger winds shaping the jets?
Authors: Nativi L , Lamb G P , Rosswog S , Lundman C , Kowal G
Published: October 2021

Jets can become collimated as they propagate through dense environments and understanding such interactions is crucial for linking physical models of the environments to observations. In this work, we use 3D special-relativistic simulations to study how jets propagate through the environment created around a neutron star merger remnant by neutrino-driven winds. We simulate four jets with two different initial structures, top-hat and Gaussian, and two luminosities. After jet breakout, we study the angular jet structures and the resulting afterglow light curves. We find that the initial angular structures are efficiently washed out during the propagation, despite the small wind mass of only ∼10−3 M⊙. The final structure depends on the jet luminosity as less energetic jets are more strongly collimated, and entrainment of baryons leads to a moderate outflow Lorentz factor (≈40). Although our jets are not specifically intended to model the outflows of the GW170817 event, we show that they can be used to produce light curves consistent with the afterglow observed in the aftermath of GW170817. Using this procedure, we show how the inferred physical parameters e.g. inclination angle, ambient particle number density, can vary substantially between independent fits of the same dataset, and appear to be sensitive to smaller details of the angular jet shape, indicating that observationally inferred parameters may depend sensitively on the employed jet models.
A comparison of 2D Magnetohydrodynamic supernova simulations with the CoCoNuT-FMT and Aenus-Alcar codes
Authors: Varma Vishnu , Müller Bernhard , Obergaulinger Martin
Published: October 2021

Code comparisons are a valuable tool for the verification of supernova simulation codes and the quantification of model uncertainties. Here, we present a first comparison of axisymmetric magnetohydrodynamic (MHD) supernova simulations with the CoCoNuT-FMT and Aenus-Alcar codes, which use distinct methods for treating the MHD induction equation and the neutrino transport. We run two sets of simulations of a rapidly rotating 35M⊙ gamma-ray burst progenitor model with different choices for the initial field strength, namely $10^{12}\, \mathrm{G}$ for the maximum poloidal and toroidal field in the strong-field case and $10^{10}\, \mathrm{G}$ in the weak-field case. We also investigate the influence of the Riemann solver and the resolution in CoCoNuT-FMT. The dynamics is qualitatively similar for both codes and robust with respect to these numerical details, with a rapid magnetorotational explosion in the strong-field case and a delayed neutrino-driven explosion in the weak-field case. Despite relatively similar shock trajectories, we find sizeable differences in many other global metrics of the dynamics, like the explosion energy and the magnetic energy of the proto-neutron star. Further differences emerge upon closer inspection, for example, the disc-like surface structure of the proto-neutron star proves high sensitivity to numerical details. The electron fraction distribution in the ejecta as a crucial determinant for the nucleosynthesis is qualitatively robust, but the extent of neutron- or proton-rich tails is sensitive to numerical details. Due to the complexity of the dynamics, the ultimate cause of model differences can rarely be uniquely identified, but our comparison helps gauge uncertainties inherent in current MHD supernova simulations.
Orbit evolution in growing stellar bars: bar-supporting orbits at the vertical ILR region
Authors: Manos T , Skokos Ch , Patsis P A
Published: October 2021

We investigate the evolution of orbital shapes at the inner Lindblad resonance region of a rotating 3D bar, the mass of which is growing with time. We evaluate in time-dependent models, during a 5 Gyr period, the importance of orbits with initial conditions known to play a significant role in supporting peanut-like structures in autonomous systems. These orbits are the central family of periodic orbits (x1) and vertical perturbations of it, orbits of its standard 3D bifurcations at the region (x1v1 and x1v2), as well as orbits in their neighbourhood. The knowledge of the regular or chaotic character of these orbits is essential as well, because it allows us to estimate their contribution to the support of a rotating bar and, more importantly, the dynamical mechanisms that make it possible. This is calculated by means of the GALI2 index. We find that orbital patterns existing in the autonomous case, persist for longer times in the more massive bar models, and even more so in a model in which the central spheroid component of our adopted galactic potential becomes rather insignificant. The peanut-supporting orbits that we find, have a regular or, in most cases, a weakly chaotic character. There are cases in which orbits starting close to unstable periodic orbits in an autonomous model behave as regular and support the bar when its mass increases with time. As a rule of thumb for the orbital dynamics of our non-autonomous models at a certain time, can be considered the dynamics of the corresponding frozen systems around that time.
Synthetic photometry of OB star clusters with stochastically sampled IMFs: analysis of models and HST observations
Authors: Orozco-Duarte Rogelio , Wofford Aida , Vidal-García Alba , Bruzual Gustavo , Charlot Stephane , Krumholz Mark R , Hannon Stephen , Lee Janice , Wofford Timothy , Fumagalli Michele , Dale Daniel , Messa Matteo , Grebel Eva K , Smith Linda , Grasha Kathryn , Cook David
Published: October 2021

We present a pilot library of synthetic NUV, U, B, V, and I photometry of star clusters with stochastically sampled IMFs and ionized gas for initial masses, Mi = 103, 104, and 105 M⊙; t = 1, 3, 4, and 8 Myr; Z = 0.014 and Z = 0.002; and log(US) = −2 and −3. We compare the library with predictions from deterministic models and observations of isolated low-mass (<104 M⊙) star clusters with co-spatial compact H ii regions. The clusters are located in NGC 7793, one of the nearest galaxies observed as part of the HST LEGUS and Hα-LEGUS surveys. (1) For model magnitudes that only account for the stars: (a) the residual |deterministic mag - median stochastic mag| can be ≥0.5 mag, even for Mi = 105 M⊙; and (b) the largest spread in stochastic magnitudes occurs when Wolf–Rayet stars are present. (2) For Mi = 105 M⊙: (a) the median stochastic mag with gas can be >1.0 mag more luminous than the median stochastic magnitude without gas; and (b) nebular emission lines can contribute with $\gt 50{{\ \rm per\ cent}}$ and $\gt 30{{\ \rm per\ cent}}$ to the total emission in the V and I bands, respectively. (3) Age-dating OB-star clusters via deterministic tracks in the U-B versus V-I plane is highly uncertain at Z = 0.014 for Mi ∼ 103 M⊙ and Z = 0.002 for Mi ∼ 103–105 M⊙. (4) For low-mass clusters, the V-band extinction derived with stochastic models significantly depends on the value of log(US). (5) The youngest clusters tend to have higher extinction. (6) The majority of clusters have multi-peaked age PDFs. (7) Finally, we discuss the importance of characterizing the true variance in the number of stars per mass bin in nature.
GRB variabilities and following gravitational waves induced by gravitational instability in NDAFs
Authors: Shahamat Narjes , Abbassi Shaharam , Liu Tong
Published: October 2021

The present work proposes a new formalism for the inner regions of a neutrino-dominated accretion flows (NDAFs) by considering the self-gravity, where the neutrino opacity is high enough to make neutrinos trapped becoming a dominant factor in the transportation of energy and angular momentum over the magneto rotational instability. We investigate the possibility of gravitational instability and fragmentation to model the highly variable structure of the prompt emission in gamma-ray bursts (GRBs). The results lead us to introduce the gravitational instability, in these inner regions, as a source of a new viscosity that is of the same functional form as that of the β-prescription of viscosity. Such a consideration brings about fragmentation in the unstable inner disc. In addition, we find the consequent clumpy structure of this area capable to account for the temporal variability of GRB’s light curve, especially for the lower choices of the parameter β, ∼10−5. Finally, we predict the formation of gravitational waves through the migration of fragments before being tidally disrupted. These waves appear to be detectable via a range of current and future detectors from LIGO to Cosmic Explorer.
Direct measurements of infrared intensities of HCN and H2O + HCN ices for laboratory and observational astrochemistry
Authors: Gerakines Perry A , Yarnall Yuki Y , Hudson Reggie L
Published: October 2021

Hydrogen cyanide (HCN) is found in a wide variety of extraterrestrial environments within and beyond the Solar system, and for that reason laboratory spectroscopists have studied this compound in many spectral regions, including the infrared (IR). However, one aspect that remains to be investigated is the intrinsic IR spectral intensities of solid HCN as opposed to relative band strengths, intrinsic intensities being needed to measure HCN abundances. Here we report measurements of IR absorption coefficients and band strengths, along with supporting refractive indices and densities, of both amorphous and crystalline HCN at two temperatures, one for interstellar work and one more relevant to the outer Solar system. Spectra are presented at both temperatures, along with optical constants that can be used in numerical models. Despite widespread and longstanding interest in and investigations of solid HCN, this is the first time that the properties we are reporting have been measured in a single laboratory, avoiding the need for estimates or to combine results from various authors. We find that our measured band strength of ∼1 × 10–17 cm molecule–1 for the C≡N vibration of HCN, in both amorphous HCN and in an H2O-rich ice, is substantially higher than an earlier estimate. Unless errors of 100 per cent can be tolerated, then our new value requires a rescaling of earlier work. Our results shed light on why HCN and other nitriles have been so difficult to identify in the solid state, in contrast to their many detections in the gas phase.
Models of metal-poor stars with different initial abundances of C, N, O, Mg, and Si – I. Bolometric corrections derived from new MARCS synthetic spectra and their implications for observed colour–magnitude diagrams
Authors: VandenBerg Don A , Edvardsson Bengt , Casagrande Luca , Ferguson Jason W
Published: October 2021

New, high-resolution MARCS synthetic spectra have been calculated for more than a dozen mixtures of the metals allowing, in turn, for variations in C:N:O, [CNO/Fe], and enhanced abundances of C, O, Mg, and Si. Bolometric corrections (BCs) for many of the broad-band filters currently in use have been generated from these spectra. Due to improved treatments of molecules that involve atoms of C, N, and O, the BCs for UV and blue passbands, in particular, differ substantially from those derived from previous MARCS models. These differences, and the effects on the BCs of varying the abundances of the metals, are shown in a number of instructive plots. Stellar evolutionary grids for −2.5 ≤ [Fe/H] ≤−0.5 have also been computed for the different mixtures. Isochrones based on these tracks are intercompared on the theoretical H–R diagram and on a few of the colour–magnitude diagrams that can be constructed from HST Wide Field Camera 3 (WFC3) F336W, F438W, F606W, F814W, F110W, and F160W observations. For the first application of these models, isochrones have been fitted to WFC3 photometry of the globular cluster NGC 6496 from the HST UV Legacy Survey, with very encouraging results.
Deep Chandra observations of diffuse hot plasma in M83
Authors: Wang Q Daniel , Zeng Yuxuan , Bogdán Ákos , Ji Li
Published: October 2021

It is widely believed that galaxy formation and evolution is regulated by stellar mechanical feedback in forms of fast stellar winds and supernova explosions. However, the coupling of this feedback with the interstellar medium remains poorly understood. We examine how the coupling may be traced by diffuse soft X-ray emission in M83 – a nearby face-on spiral galaxy undergoing active star formation, based chiefly on 729 ks Chandra observations. Our main findings are (1) the X-ray emission is enhanced not only along the galaxy’s grand spiral arms, but also clearly in their downstreams; (2) the spectrum of the emission can be well characterized by a supersolar metallicity plasma with a lognormal temperature distribution, plus an X-ray absorption of a lognormal column density distribution; and (3) the intensity of the emission is strongly anticorrelated with the dust obscuration seen in optical images of the galaxy. These findings suggest (A) the morphology of the X-ray emission is likely due to the convolution of the feedback heating of the plasma with its thermal and dynamical evolution; (B) the X-ray emission, accounting for $\sim 10{{\ \rm per\ cent}}$ of the feedback energy input rate, probably traces only the high-energy tail of the radiation from the plasma; (C) a good fraction of the recent star-forming regions seems sufficiently energetic to produce multi-phased outflows, likely responsible for much of the dust obscuration and X-ray absorption. Direct confrontation of the findings with theories/simulations could help to understand the underlying astrophysics of the coupling and how the hot plasma shapes the interstellar medium.