bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of different fat sources and vitamin E status/levels on growth performance, antioxidant status, carcass characteristics, fatty acid profile, and meat quality of pigs grown to heavy slaughter weight at 150 kg
Authors: Wang Ding , Jang Young Dal , Rentfrow Gregg K , Monegue Harold J , Azain Michael , Lindemann Merlin
Published: July 2019

Two experiments were used to evaluate the potential interaction of fat source and vitamin E (VE) in heavy slaughter weight pigs. In Experiment 1, a total of 64 individually-fed pigs (28.41 ± 0.83 kg) were randomly assigned to 8 dietary treatments in a 4×2 factorial arrangement. Fat treatments included cornstarch (CS), tallow (TW), corn-oil (CO), and coconut-oil (CN). VE treatments were dietary α-tocopheryl-acetate (ATA) at 11 and 200 ppm. In Experiment 2, a total of 72 individually fed pigs (28.55 ± 1.16 kg) were randomly assigned to 12 dietary treatments in a 2×6 factorial arrangement. Fat treatments were TW and CO. VE treatments included four levels of ATA (11, 40, 100, and 200 ppm) and two levels of mixed tocopherols (primarily γ-tocopherol; 40 and 100 ppm). VE deposition, growth performance, and meat quality were measured in both experiments. In both experiments, interaction between fat sources and VE were detected (P < 0.01) on plasma VE concentration, which increased (P < 0.01) with time and with increasing dietary VE, but increased faster (P < 0.05) in pigs fed with CN and TW compared to pigs fed CS and CO. Compared to CO, more saturated dietary fat sources (CN and TW) led to firmer belly (P < 0.01), which had more (P < 0.01) SFA and MUFA while less (P < 0.01) PUFA. In Experiment 1, increasing dietary ATA decreased (P < 0.05) FCR in Phase 4 and Phase 5. In Experiment 2, increasing dietary ATA increased overall ADG (linear, P = 0.02), with an interaction (P < 0.05) with fat sources on cumulative ADG during Phase1-4, wherein pigs fed CO, but not TW, had increased ADG with increasing dietary ATA. Increasing dietary ATA increased (quadratic, P < 0.05) liver SOD activity, and decreased (quadratic, P < 0.05) liver MDA content. The oxidative stability of loin was improved (P < 0.01) when dietary ATA increased over 40 ppm. In summary, both dietary fat source and VE supplementation affected the response measures.
Nutritional intervention for the intestinal development and health of weaned pigs
Authors: Liu Yanhong
Published: July 2019

Weaning imposes simultaneous stress, resulting in reduced feed intake and growth rate and increased morbidity and mortality of weaned pigs. Weaning impairs the intestinal integrity, disturbs digestive and absorptive capacity, and increases the intestinal oxidative stress and susceptibility of diseases in piglets. The improvement of intestinal development and health is critically important for enhancing nutrient digestibility capacity and disease resistance of weaned pigs, therefore, increasing their survival rate at this most vulnerable stage and overall productive performance during later stages. A healthy gut may include but not limited several important features: a healthy proliferation of intestinal epithelial cells, an integrated gut barrier function, a preferable or balanced gut microbiota, and a well-developed intestinal mucosa immunity. Burgeoning evidence suggested nutritional intervention are one of promising measures to enhance intestinal health of weaned pigs, although the exact protective mechanisms may vary and are still not completely understood. Previous research indicated that functional amino acids, such as arginine, cysteine, glutamine, or glutamate, may enhance intestinal mucosa immunity (i.e. increased sIgA secretion), reduce oxidative damage, stimulate proliferation of enterocytes, and enhance gut barrier function (i.e. enhanced expression of tight junction protein) of weaned pigs. A number of feed additives are marketed to assist in boosting intestinal immunity and regulating gut microbiota, therefore, reducing the negative impacts of weaning and other environmental challenges on piglets. The promising results have been demonstrated in antimicrobial peptides, clays, direct-fed microbials, micro-minerals, milk components, oligosaccharides, organic acids, phytochemicals, and many other feed additives. This review summarizes our current understanding of nutritional intervention on intestinal health and development of weaned pigs and the importance of mechanistic studies focusing on this research area.
58 Feed, feeding levels, and tissue analytics of vitamins: Observations from the field
Authors: Hinson Buddy , Moser Ronny , Ackerman Matthew , Main Rodger
Published: July 2019

Targeted vitamin inclusion rates within swine diets are typically at levels 2–7 times that of the known published requirements. These increased rates are mainly looked at as an “insurance policy” since very little vitamin research has been conducted in the last two decades and swine growth rates and reproductive traits have increased substantially during this same time. When production issues such as broken bones, downer animals, or sudden deaths occur, vitamin analysis is commonly done on feed, serum, or tissue samples. This discussion will shed light on observations around vitamin analytics that include variation due concentration within product, lab-to-lab variation, and within lab variation. In addition to discrepancies found in feed, serum and liver sample vitamin analytics do not seem to present a clear picture. Across multiple phases of production, these analysis consistently fall well below what would be considered to be reference values that are reported by diagnostic labs. This occurs not only in sick animals but also in animals that appear to be in perfect health and are consuming adequate levels of feed. In order to determine the impact of vitamin supplementation on performance and serum/tissue vitamin status, five trials across sows, nursery, and grow-finish swine were conducted. The goal of these trials is not to determine the various vitamin requirements, but to begin the conversation in order to help establish new reference values that are applicable to today’s genetics, vitamin supplementation levels, and rearing environments. Hopefully this presentation will lead to further questions and discussions within the industry regarding level of vitamin supplementation and the subsequent impacts on performance and serum/tissue vitamin status.
68 Genomic prediction accuracies of vulva size traits in Landrace and Yorkshire gilts
Authors: Corredor Flor Anita , Leach Richard J , Ross Jason W , Keating Aileen F , Serão Nick V L
Published: July 2019

Recent results indicated that vulva size measured prior to puberty may be predictive of reproductive performance in sows. Therefore, the objective of this study was to estimate genomic prediction accuracies for vulva size traits in purebred gilts. A total of 1,185 Landrace (n = 477) and Yorkshire (n = 708) gilts originated from two different lines were used in this study. All animals had vulva size measurements taken at an average 21.5 weeks of age (SD = 5.8). Measurements included vulva width (VW), vulva height (VH), and vulva area (VA). Genotype data (Geneseek GGP-HD) was available for all animals, for ~40K SNPs. Marker allele substitution effects were estimated using Bayes-B (pi = 0.99) in a model including the fixed effects of contemporary group, line, breed (for multi-breed analysis only) and body weight (covariate), and the random effect of SNPs. Genomic prediction accuracies were estimated using three training and validation strategies: between-breed, within-breed (4 and 6 cross-validation folds for Landrace and Yorkshire, respectively), and multi-breed (10-fold cross-validation, using one-fold per breed for validation at a time). Between-breed accuracies were low and consistently negative, with -0.02, -0.10, and -0.05 in Landrace and -0.05, -0.04 and -0.03 in Yorkshire, for VW, VH, and VA, respectively. Within Landrace, these were moderate, with 0.35 (VW), 0.42 (VH), and 0.56 (VA), whereas lower accuracies were obtained for Yorkshire, with 0.07 (VW), 0.20 (VH), and 0.14 (VA). Multi-breed accuracies were low with 0.14 (VW), 0.14 (VH), and 0.24 (VA) for Landrace, and 0.03 (VW), 0.16 (VH), and 0.09 (VA) for Yorkshire. These results indicate that genomic selection for vulva size traits is possible in Landrace, but limited in Yorkshire gilts. The low between- and multi-breed results suggest that QTL for these traits are in opposite phases between breeds and/or do not segregate in both breeds. Financial support from the Iowa Pork Industry Center is appreciated.
76 Effects of replacing monensin and tylosin in finishing cattle diets with essential oils and/or benzoic acid on growth performance and carcass characteristics
Authors: Wang Lydia M , Mandell Ira B , Bohrer Benjamin M
Published: July 2019

This study examined the effects of replacing monensin and tylosin with essential oils and/or benzoic acid in finishing cattle diets on growth performance, feed efficiency, and carcass characteristics. Crossbred steers (n = 68; BW = 539 ± 4 kg) were blocked by starting weight into three blocks and were assigned to 1 of 5 finishing diets: no additional supplement (CON), monensin/tylosin (M/T), essential oils (EO), benzoic acid (BA), or a combination of essential oils and benzoic acid (COMBO). Steers were housed with two dietary treatments represented in seven pens, while an eighth pen only housed steers fed the CON diet. Individual animal feed intake was collected using an Insentec feeding system, so steer was the experimental unit for all analyses. Data were analyzed using a randomized complete block design with fixed effect of treatment and random effect of block. Final weight, average daily gain, and dry matter intake were similar (P > 0.12) among treatments. Gain to feed ratio differed (P = 0.05) among treatments, specifically steers fed the M/T diet had greater G:F compared with steers fed CON, EO, and COMBO diets. For carcass characteristics, there were no significant treatment differences in hot carcass weight (P = 0.19), dressing percentage (P = 0.62), ribeye area (P = 0.49), fat thickness (P = 0.84), calculated yield grade (P = 0.91), marbling score (P = 0.07), and yield grade (P = 0.43). Quality grade differed (P = 0.05) among treatments, with steers fed the EO diet having a lower quality grade than all other dietary treatments. Overall, steers supplemented with essential oils and(or) benzoic acid had similar gain, feed intake, and carcass parameters as steers fed CON, while steers fed M/T had slightly improved feed efficiency compared to all other treatments.
70 Effects of copper, zinc, and manganese intake in late gestation on milk, cow plasma, and calf plasma trace mineral concentrations post-calving in beef cattle
Authors: Stephenson Emma L , Rathert Abigail R , Tucker Heather , Meyer Allison M
Published: July 2019

Multiparous, fall-calving beef cows [n = 48; 649 ± 80 (SD) kg BW; 5.3 ± 0.5 BCS] were individually-fed tall fescue-based hay (12.2% CP, 61.5% NDF) and supplemented to meet/exceed nutrient recommendations except Cu, Zn, and Mn. From approximately 90 d pre-calving to 11 d post-calving, cows received: no additional Cu, Zn, or Mn (CON); Cu, Zn, and Mn sulfates (ITM) or metal methionine hydroxy analogue chelates (CTM, MINTREX®, Novus International) supplying 133% NASEM recommendations; or Cu, Zn, and Mn sulfates and chelates supplying 100% recommendations (reduce and replace, RR). Treatment, sampling day, their interaction, and breeding group were fixed effects with cow as the experimental unit. Colostrum and milk Cu and Mn and plasma Mn were generally not detectable. Colostrum Zn was greater (P ≤ 0.03) in CTM and ITM than CON and RR. All treatments had greater (P < 0.001) colostrum Zn than d 35 milk, which was greater (P ≤ 0.03) than d 60. Treatment did not affect (P ≥ 0.19) cow or calf plasma Cu or Zn post-calving. Calf plasma Zn decreased (P ≤ 0.02) from 0 to 35 d of age. Calf plasma Cu increased (P < 0.01) from 0 to 35 d, then decreased (P = 0.01) from 35 to 60 d. Cow plasma Zn and Cu were greater (P ≤ 0.02) at lactation d 35 and 60 than 1 h post-calving. Pearson correlation coefficients were used to determine relationships among cow and calf mineral status. There were weak positive correlations (P ≤ 0.06) between calf plasma and milk Zn at d 35 and 60. Cow and calf plasma Zn immediately post-calving had a weak negative correlation (P = 0.04). These results indicate greater Zn concentration in beef cow colostrum than milk, and suggest calf circulating Zn is partially dependent on milk Zn concentration.
83 Lifelong dynamics of the swine gut microbiome: from birth to market
Authors: Wang Xiaofan , Tsai Tsung Cheng , Maxwell Charles V , Zhao Jiangchao
Published: July 2019

Despite the recent advances in the swine gut microbiomes during different growth stages, a comprehensive longitudinal study of the lifelong dynamics of the swine gut microbiome is lacking. To fill this gap of knowledge, we selected seventeen piglets (PIC29*380) that were born on the same date from three sows. We collected a total of 274 rectal swabs during lactation (d 0, 11, 20), nursery (d 27, 33, 41, 50, 61), growing (d 76, 90, 104, 116), and finishing (d 130, 146, 159, and 174) stages. Samples were extracted using the Powersoil DNA isolation kit (Qiagen, Hilden, Germany) and sequenced with an Illumina Miseq sequencer targeting the V4 region of the 16 S rRNA gene. Sequences were analyzed with the Deblur algorithm in the QIIME2 package. In general, alpha diversity including community richness (e.g., number of observed features, Chao1) and diversity (e.g., Shannon Index) showed an overall trend of increasing from lactation to the finishing stage (P < 0.01). Gradual and significant changes in community structures were also observed along the four growth stages (ANOSIM, R = 0.66; P < 0.01). Non-parametric permutational multivariate analysis of variance shows that main factors driving the lifelong community dynamics included age and diet. Seventeen phylum members were discovered in the lifelong pig gut microbiome with Firmicutes and Bacteroidetes being the most abundant phyla. LEfSe analysis revealed 63 bacterial features that are stage specific. By using a regressing tree based Random Forest model we identified five bacterial features that are associated with swine growth performance including features 26 (Turicibacteraceae Turicibacter), 27 (Clostridium butyricum), 18 (Clostridiaceae), 19 (Clostridium perfringens) and 4 (Clostridiaceae). Characterization of the lifelong dynamics of 17 healthy pigs from birth to market provides a foundation for gut microbiome studies focusing on swine development, health and growth performance.
93 Host genetics help shape the rumen microbiome in beef cattle
Authors: Abbas Waseem , Howard Jeremy T , Paz Henry A , Hales Kristin E , Wells James E , Kuehn Larry A , Spangler Matthew L , Erickson Galen E , Fernando Samodha C
Published: July 2019

In this study, we investigated the degree to which host genetics shape the rumen microbiome. Complex and diverse microbial communities can alter the nutrient profile available to the animal and subsequent performance. Studies of the gut microbiome have demonstrated that host genotype influences gut microbial species composition. Therefore, microbial species composition in the rumen may be a complex trait that manifests through the convergence of host-genetics and environmental factors. To test this hypothesis, we collected rumen contents and blood samples from 586 beef cattle on different diets from two locations. The rumen samples were used to sequence the V4 region of the 16S rDNA on an Illumina MiSeq platform. Animals were genotyped with various platforms and a common set of 61,974 SNP were used to conduct a genome-wide association study (GWAS) using the microbiome (OTUs, families and phyla) as response variables. The GWAS was performed using Bayesian GBLUP fitting fixed effects of cohort (location and date), and the first 2 principle components to account for population stratification. Median posterior genomic heritability estimates were 0.110, 0.124, and 0.141 at the OTU, family and phylum taxonomic level, respectively. The top 8 1-Mb windows for OTUs, families and phyla were located on 7 different chromosomes. These regions affect the rumen microbiota in multiple ways; some (chromosome 19; position 3.0–4.0 Mb) are associated with closely related taxa (Prevotellaceae, Paraprevotellaceae, and RF16), some (chromosome 27; position 3.0–4.0 Mb) are associated with distantly related taxa (Prevotellaceae, Fibrobacteraceae, RF16, RFP12, S24-7, Lentisphaerae, and Tenericutes) and others (chromosome 23; position 0.0–1.0) controlling both related and unrelated taxa. Overall, the 8 regions identified control 11 different families and 6 different phyla. This study shows that host genetics can affect rumen bacterial community members and points towards the possibility that genomics can be used to manipulate the rumen microbiome.
95 The effect of incremental dietary energy density from 13.8 to 15.9 MJ DE/kg on piglet vitality and growth to weaning
Authors: Rooney Hazel B , O’Driscoll Keelin , O’Doherty John , Lawlor Peadar
Published: July 2019

Genetic selection for hyper-prolificacy in sows has resulted in a significant increase in the number of piglets born alive per litter; however, a consequence has been a reduction in piglet vitality and survival. Increasing lactation dietary energy density can increase sow energy intake and the survival and growth of piglets from large litters. The objective of this experiment was to investigate the effect of increasing sow lactation dietary energy density on piglet vitality and growth to weaning. At d 108 of gestation, sows (n = 115) were blocked by parity, BW and backfat, and randomly assigned to one of four energy density diets until weaning; 13.8, 14.5, 15.2 and 15.9 MJ DE/kg. At birth, body temperature, IUGR traits and muscle tone were recorded in piglets. At 24 h, piglet body temperature and blood glucose concentration were recorded. Piglets were weighed throughout lactation. Data were analysed using the mixed models procedure in SAS (v.9.4). Average daily energy intake was highest for sows fed 15.9 MJ DE/kg compared to sows fed 13.8, 14.5 and 15.2 MJ DE/kg (115.1 v 98.92, 102.33, 107.83MJ DE/d±1.51; P < 0.01). Piglets from sows fed 13.8 and 15.9 MJ DE/kg were more likely to have more IUGR traits and a higher muscle tone score, respectively, compared with all other treatments (P < 0.01). The 24 h body temperature was higher for piglets from sows fed 13.8 and 15.2 MJ DE/kg than piglets from sows fed 15.9 MJ DE/kg (35.47 and 35.48 v 35.22⁰C±0.05; P < 0.01). Glucose concentration was higher for piglets from sows fed 14.5 MJ DE/kg than piglets from sows fed 15.9 MJ DE/kg (4.70 v 4.15mg/ml±0.14; P < 0.01). Litter weaning weight was heaviest for sows fed 13.8 MJ DE/kg compared with sows fed 14.5 MJ DE/kg (92.90 v 84.97kg±1.59; P < 0.01). In conclusion, feeding lactating sows 15.9 MJ DE/kg increased daily sow energy intake and improved piglet development.
97 Impact of diet on shelf life of beef steaks
Authors: Bland Nicolas , Ribeiro Felipe , Herrera Nicolas , Hart Kellen , Henriott Morgan , Calkins Chris
Published: July 2019

This research was conducted to evaluate the effects of feeding different types of processed corn, with or without distiller grains, on shelf life of beef steaks. Steers (n = 240) were finished on Dry Rolled Corn (DRC), DRC + 30% Dried Distillers Grains (DDGS), Steam Flaked Corn (SFC), or SFC + 30% DDGS. Cattle were fed 10 per pen. Only pens with upper 2/3 Choice and Select-grade carcasses were sampled, with a goal of two (and a minimum of one) of each grade per pen. Three pens per treatment met the selection criterion and 36 carcasses were chosen (21 upper 2/3 Choice and 15 Select). Pen was considered the experimental unit. Both strip loins were collected from each carcass, halved, and aged for 2, 9, 16, or 23 d. After aging, steaks were placed under retail display for 7 d. Discoloration and objective color (L*, a*, and b*) were determined daily and lipid oxidation (thiobarbituric acid reactive substances; TBARS) was measured after 0 and 7 d of retail display. Fatty acid profile of the lean was obtained. Minimal to no impacts of grade on color and lipid oxidation were found (P > 0.05). Steaks from cattle fed DRC were statistically or numerically lowest for discoloration and C18:2 (linoleic acid) and highest for redness compared to the other treatments (P < 0.05). Conversely, steaks from cattle fed SFC+DDGS had the most C18:2 and the worst (least desirable) values for discoloration and redness toward the end of retail display (P < 0.05). Steaks from cattle fed DRC had the lowest TBARS values, while steaks from cattle fed SFC+DDGS had the highest TBARS values (P < 0.05). These data suggest that steaks from cattle fed SFC+DDGS oxidize, discolor, and lose redness more quickly under retail display than steaks from cattle fed DRC, likely due to changes in fatty acid content.
100 Young Scholar Presentation: Effects of sex, sire line, and packaging method on the relationships among early postmortem loin quality and aged loin and pork chop quality characteristics
Authors: Lowell Jessica , Boler Dustin D , Dilger Anna C
Published: July 2019

A challenge in the pork industry is that packers assess pork loin quality at ~1d postmortem, while consumers assess quality after a period of postmortem aging. Rapid line speed necessitates rapid noninvasive assessment of quality by packers as indicators of aged (~14d) quality. However, it is unclear if this assessment is representative of quality observed by consumers at purchase. It is also unclear if correlations differ between barrows and gilts, sire lines, or packaging methods. Therefore, several studies were conducted to address these questions. Harsh et al. (doi.org/10.1093/jas/sky183) used meta-analyses techniques to establish correlations between early and aged pork loin quality. Data sets encompassed ~3,957 loins. Early instrumental and visual color were moderately correlated ( ≥ 0.38) with aged instrumental and visual color. No early quality parameters were correlated ( ≤ 0.36) with instrumental or sensory tenderness. With correlations between early and aged quality established, differences between factors of interest were tested. Correlations were compared using a Fisher’s z test. First, barrows and gilts were compared using a single sire line and raised in commercial conditions. Correlations between early and aged quality did not differ (P > 0.05) between barrows and gilts. Correlations between Pietrian- and Duroc-sired pigs were compared to determine correlation difference between sire lines using pigs from two different production focuses (meat quality vs. lean growth). Correlations between early ventral L* and aged chop L*, and early visual color and aged ventral L* differed (P ≤ 0.03) between Pietrain- and Duroc-sired pigs. However, the majority of correlations did not differ (P > 0.05) between sire lines. Correlations between packaging methods were compared by assigning boneless pork loins to one of two packaging methods, intact loins or case-ready chops. The correlations between early and aged quality traits did not differ (P > 0.05) between packaging methods. Overall, packers can use the same early quality characteristics to select for aged quality, regardless of sex, sire line or packaging method.
113 Pathogen shedding in feces of nursery swine
Authors: Wells James E , Berry Elaine , Oliver William T
Published: July 2019

Foodborne pathogens are a significant cause of disease in humans, and meat-producing animals are reservoirs for a variety of these pathogens. Consumption of contaminated meat or meat products is a major route for human infection, but indirect infection can arise from water and other foods contaminated with animal waste. Hence, reducing colonization and fecal shedding is important for sustaining a safe food chain and environment. Swine are potential reservoirs for several of the foodborne and related pathogens. Piglets can be colonized soon after birth, and the transition to the nursery is a stressful period. At farrowing, Campylobacter is a major pathogenic bacterium observed in feces of piglets, and infection can increase significantly during the nursery phase of production. Moreover, pathogen shedding has been associated with reduced performance. Dietary supplementation of carbodox with copper sulfate or lysozyme from egg whites has been shown to reduce Campylobacter shedding in piglets after weaning, but neither treatment significantly affected potentially pathogenic E. coli shedding. Supplementation with a commercial Lactobacillus acidophilus fermentation product had limited effect on pathogen shedding, whereas tiamulin had no significant effect. Nursery swine are prone to pathogen colonization, and there are dietary supplements shown to be effective for reducing shedding during this important phase of swine production.
125 Effects of copper hydroxychloride and increasing concentrations of dietary fat on growth performance, total tract endogenous loss of fat, and apparent total tract digestibility of fat by growing pigs
Authors: Espinosa Charmaine D , Fry Robert Scott , Kocher Matthew , Stein Hans H
Published: July 2019

Two experiments were conducted to test the hypothesis that Cu hydroxychloride (IntelliBond C, Micronutrients, LLC, Indianapolis, IN) improves gain:feed of pigs by increasing apparent total tract digestibility (ATTD) of fat. In experiment 1, 144 pigs (15.40 ± 2.39 kg) were allotted to 1 of 6 treatments. Four diets contained 0, 2, 4, or 6% choice white grease (CWG) and 2 diets contained 150 mg/kg Cu from Cu hydroxychloride and 0 or 2% CWG. Data for growth performance were analyzed by contrast statements, and Cu equivalency of CWG was determined using prediction equations derived from regression procedures using SAS. Results indicated that gain:feed linearly increased (P ≤ 0.05) as CWG concentration increased in diets (Table 1). Supplementation of Cu hydroxychloride to diets improved (P ≤ 0.05) gain:feed of pigs, which resulted in a CWG equivalence of 2.8 to 3.8% for 150 mg/kg Cu hydroxychloride. In experiment 2, 80 pigs (18.24 ± 1.81 kg) were housed individually in metabolism crates and randomly allotted to 1 of 10 diets. Eight diets contained 0, 15, 30, or 45% distillers dried grains with solubles (DDGS) and 0 or 150 mg/kg Cu hydroxychloride. Two additional diets contained 2% CWG and 15% DDGS and 0 or 150 mg/kg Cu hydroxychloride. Feces were collected for 4 d after 5 d adaptation. Data were analyzed by contrast statements and regression procedures using SAS. Supplementation of Cu to diets improved (P ≤ 0.05) ATTD of fat by 20% due to reduced (P ≤ 0.05) endogenous loss of fat (from 11.23 to 7.14 g/kg dry matter intake), but did not affect energy digestibility or true total tract digestibility of fat. In conclusion, supplementation of 150 mg/kg Cu hydroxychloride to diets improved gain:feed of pigs with a CWG equivalence of 2.8 to 3.8%, but this was not due to improved energy digestibility.
132 The impact of F18 ETEC challenge on intestinal integrity and immune response of nursery pigs, and the potential protective effects of direct-fed microbial blends
Authors: Becker Spenser L , Li Qingyun , Burrough Eric R , Patience John F
Published: July 2019

The objectives were to investigate the impact of an F18 ETEC challenge on localized immune responses and intestinal function of piglets, and to evaluate potential protective effects of direct-fed microbial blends (DFM1 and DFM2). Thirty-six pens of 3-wk old weaned piglets (2 pigs/pen; BW=6.42 ± 0.22 kg; L337 X Camborough, PIC, Hendersonville, TN) were assigned to one of four treatments: 1) NC: Non-challenged (n = 10), 2) PC:F18 ETEC challenged (n = 10), 3) PC+DFM1 (n = 8) or 4) PC+DFM2 (n = 8). Pigs were either sham-infected with saline or orally challenged with ETEC on d 7 (d 0 post-inoculation, dpi). On dpi 10, ileal mucosa was collected for disaccharidase quantification and whole ileal tissue was collected for immune marker and tight junction protein analysis via qRT-PCR. Data were analyzed using PROC MIXED of SAS (9.4) with dietary treatment as a fixed effect and pen as the experimental unit. There were no differences in lactase activity (P > 0.10). Sucrase and maltase activity increased in PC vs. NC (P < 0.05). Ileal mRNA abundance of zonula occludens-1 (ZO-1) decreased in PC and DFM1 compared with NC (P < 0.05). DFM2 increased ZO-1 compared with PC (P < 0.05). There was no difference in claudin-1 mRNA (P > 0.10). Cluster of differentiation 14 (CD14) mRNA was elevated in challenged pigs compared with NC (P < 0.05). Interleukin-10 (IL-10) tended to be elevated in both DFM treatments compared with PC (P < 0.10). All challenged treatments tended to have elevated tumor necrosis factor-a (TNFa) abundance vs. NC (P < 0.10). There were no differences in IL-1b, IL-6, or toll-like receptor 4 (TLR4) mRNA abundance (P > 0.10). In conclusion, the ETEC challenge increased CD14, tended to increase TNFa, and decreased ZO-1 mRNA abundance, indicating activation of intestinal inflammation and impaired intestinal barrier integrity. DFM2 may improve intestinal barrier integrity impaired by ETEC as evidenced by increased ZO-1 mRNA.
131 Effects of Bacillus spp. probiotics on systemic immunity and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli
Authors: He Yijie , Kim Kwangwook , Jinno Cynthia , Wu Zhaohai , Whelan Rose , Doranalli Kiran , Liu Yanhong
Published: July 2019

The experiment was conducted to investigate the effects of Bacillus spp. on systemic immunity and intestinal health of weaned pigs infected with F18 E. coli. Weaned pigs (n = 36, 7.61 ± 0.40 kg BW) were randomly allotted to one of three treatments: a control diet and two diets supplemented with 500 mg/kg of Bacillus spp. strain 1 (PRO1) or strain 2 (PRO2). The experiment was conducted for 28 d, including 7 d before and 21 d after the first E. coli inoculation (d 0). The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 consecutive days. Serum samples were collected on d 0 before inoculation, and d 3, 7, 14, and 21 post-inoculation (PI) to measure inflammatory meditators. All pigs were euthanized on d 21 PI to collect tissue samples for gut morphology, bacterial translocation, and gene expression analysis. Data were analyzed using the Mixed Procedure of SAS. Pigs in PRO1 had reduced (P < 0.05) serum haptoglobin on d 3 and d 6 PI compared with pigs in control. Supplementation of PRO1 or PRO2 reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO1 had greater (P < 0.05) ileal villi height than pigs in control. Pigs in PRO2 had greater (P < 0.05) sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in control. Supplementation of PRO1 also up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa but reduced (P < 0.05) COX2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with control. In conclusion, supplementation of Bacillus spp. may reduce systemic inflammation and enhance intestinal health of weaned pigs infected with F18 E. coli.
150 A bio-active, mineral-based feed additive improved growth performance and reduced severity of diarrhea in weanling pigs challenged with enterotoxigenic Escherichia coli
Authors: Sinn Sue , Song Ran , Beckler Dana , Musser Rob , Friesen Kim
Published: July 2019

A mineral-based feed additive, NutriQuest Protect™, was evaluated in five artificial Enterotoxigenic Escherichia coli (ETEC) challenge experiments to determine the effects on pig growth performance, fecal consistency and immune response. The five experiments were conducted following a similar procedure and utilized a total of 232 weanling pigs (19 d of age) assigned to one of three experimental treatments: non-challenged control (NC), challenged control (CC), and challenged pigs fed Protect at 4.0 g/kg (CP) with 36, 36, and 44 pens per treatment, respectively. Pharmacological ZnO or medications were not included in any diets. Pigs were allowed a 7-d adaptation period following weaning, orally inoculated with E. coli K88 or F18 on 0 d post-inoculation (dpi) and 1-dpi. Studies were concluded on 4-dpi. Pig BW and feed disappearance were measured on 0-dpi and 4-dpi. Serum samples were collected on 0 and 4-dpi to measure porcine proinflammatory cytokines. Fecal scores were measured daily over the challenge period. Data from the five experiments were compiled for meta-analysis using the MIXED procedure of SAS. The NC pigs had a greater ADG (0.09 vs. -0.01 kg/d, P = 0.002), ADFI (0.24 vs. 0.21 kg/d, P = 0.09), and final BW (6.8 vs. 6.5 kg, P < 0.05). Diarrhea frequency was significantly higher in CC pigs compared with pigs on CP and NC treatments (28.5 vs. 18.7 vs. 5.3%, P < 0.05). Results from the five experiments suggest that NutriQuest Protect™ improves growth performance and reduces inflammation and diarrhea in weaned pigs artificially challenged with E. coli K88 or F18.
151 Effects of carbohydrate source on performance and gastrointestinal microbiota in nursery pigs
Authors: Kinstler Sydney , Li Yanshao , Miller Phillip , Burkey Thomas E , Trenhaile-Gannemann Melanie , Fernando Samodha C , Knoell Allison , Tom Wesley A
Published: July 2019

An experiment was conducted to determine the effects of carbohydrate source on growth performance and gastrointestinal microbiota in nursery pigs. Ninety-six pigs weaned at 21 d were randomly allotted to 16 pens (6 pigs/pen; 4 pens/treatment) with sex being represented evenly in each pen. Pigs had ad libitum access to feed and water for the 2-phase nursery period (28 d). Dietary treatments included: 1) NutrisureTM(a food-grade cooked cereal grain product), 2) oatmeal (food-grade oatmeal grain), 3) steam-rolled oats (SRO), or 4) dried-distillers grains as a negative control (DDGS). Ileal and colonic digesta and mucosa samples were collected on d 0, 14, and 28 (n = 6, 32, and 32, respectively) for analysis of microbial species using IlluminaTMnext generation sequencing. The DNA sequences were filtered through the DADA2 pipeline, Mothur, and QIIME. Pig body weights and feeder weights were also measured on d 0, 7, 14, 21, and 28. Pigs fed DDGS had lower average daily feed intake (ADFI) compared to other treatments during phase 1 (P < 0.05), but there were no differences in average daily gain (ADG) or gain:feed (G:F) among treatment. There were no differences among treatments for ADFI, ADG, or G:F for phase 2 or the overall experimental period. Microbial composition differed significantly among location in the gastrointestinal tract (P < 0.01) and among the digesta and mucosa samples extracted from the ileum and colon. There were no significant changes in the microbiota among diets (P > 0.1). Over time, there was a trend (P = 0.111) for the microbiota to vary; however, there was no interaction between the diet and sampling timepoint. In conclusion, carbohydrate sources did not affect gastrointestinal microbiota composition, ADFI, ADG, or G:F overall in this experiment. However, feed intake during phase 1 differed between DDGS and oat-based carbohydrate sources and microbial species were different between digesta and mucosa in the ileum and colon.
152 Effects of dietary lactose level on nursery pig performance
Authors: Frank Jason , Sholtz Emily , Neill Casey , Jong Jon De
Published: July 2019

Lactose is a critical nutrient in post weaning diets to help pigs transition from sows’ milk to dry feed. The objective of this study was to evaluate the effects of increasing dietary lactose level on nursery pig performance. For this trial 1,080 weaned pigs (PIC 359 x 1050; BW = 6.24 kg; 21 d) were fed 5 lactose programs using a feed budget. Program A = 24, 18, 7%; B = 20, 14, 5%; C =16, 10, 3%; D = 12, 6, 1%; and E = 8, 2, 0% lactose for Phase 1, 2, and 3; respectively. The feed budget for Phase 1 (d 0–7), 2 (d 7–14), and 3 (d 14–20) was 0.91, 3.4, and 4.5 kg/pig; respectively. A common Phase 4 (d 20–48) diet (0% lactose) was fed ad libitum. There was a quadratic response to lactose level in treatments A through E for Phase 1 ADFI (89, 71, 73, 73, 89 g/d; respectively, P = 0.034) and G:F (1.09, 1.33, 1.14, 1.15, 0.91; respectively, P = 0.042). Treatment A through E Phase 1 ADG was 100, 95, 91, 82, and 82 g/d, and Phase 2 ADG (Linear, P = 0.023) was 322, 313, 318, 304, and 295 g/d; respectively. The result was a linear trend for increased BW at the end of Phase 2 (P = 0.10) for treatments A through E (9.21, 9.10, 9.16, 9.00, 8.86 kg; respectively). Although feed cost/pig increased as lactose level increased (Linear, P = 0.041), there was no significant response in margin over feed cost/pig during the overall nursery period for treatments A through E ($15.31, $16.41, $16.22, $15.87, $16.04; respectively). In conclusion, pig performance improved during Phase 1 and 2 with increasing level of dietary lactose. These results confirm previous research showing the importance of dietary lactose in weaned pig diets.
162 The interactions between dietary fiber and lipid sources alter the predicted production and absorption of caecal and colorectal volatile fatty acids in growing pigs
Authors: Ndou Saymore Petros , Kiarie Elijah , Walsh Maria , de Lange Cornelis , Martin Nyachoti C
Published: July 2019

A combination of in vivo and in vitro fermentation methodologies was used to determine the interactive effects of dietary fiber (DF) and lipid types on volatile fatty acids (VFA) production and absorption, and organic matter (OM) fermentability in the cecum and colorectal tract of pigs. Eight ileal- and caecal-cannulated Yorkshire barrows were fed either pectin- or cellulose-containing diets that were supplemented with either corn oil or beef tallow in two independent Youden squares with a 2×2 factorial arrangement of treatments (n = 6). Ileal and caecal digesta were collected, freeze-dried and fermented using inoculum from fresh caecal digesta and feces, respectively, to determine VFA production and absorption, and fermentability of OM. There were interactions (P < 0.001) between DF and lipid types observed in which the addition of corn oil increased the quantity of caecal and colorectal acetic acid production and caecal acetic absorption, caecal butyric production, predicted caecal OM fermentability, and the predicted colorectal propionic acid in pectin diets but did not have effects in cellulose diets. The addition of beef tallow increased (P < 0.001) the production of caecal butyric and propionic acids during in vitro fermentation in cellulose diets and fermentability of OM in pectin diets. The interactions between DF and lipids on gastrointestinal fermentation largely depends on the degree of saturation of fatty acids in dietary lipids. The addition of beef tallow selectively decreased the production and absorption of individual SCFA in pectin and cellulose diets but increased caecal butyric and propionic acid production in cellulose diets and the fermentability of OM in pectin diets.
170 Trends in mycotoxin contamination in the united states corn
Authors: Curry Shelby , Hendel Erika G , Gott Paige , Murugesan G R , Hofstetter-Schähs Ursula
Published: July 2019

Mycotoxins are harmful secondary fungal metabolites and are of key concern to food and feed safety globally. In addition to compromised performance, mycotoxins negatively impact animal health. Although classic signs such as decreased feed intake and vomiting are known in the field as indicators for exposure, mycotoxins act as predisposing factors for diseases by immune suppression, causing inflammation, and modulating the gastrointestinal environment, even at low levels. This survey presents mycotoxin levels of corn samples from the 2018 harvest and compares these levels with those in previous years. New crop corn samples from various sources, were submitted starting from mid-August 2018, and consisted of corn (70%), corn silage (18%), and corn byproduct (12%). Samples were analyzed utilizing the liquid chromatography and tandem mass spectrometry (LC-MS/MS) method for six major mycotoxin groups: aflatoxins (Afla), type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin-A (OTA). Data are presented for major mycotoxin classes in Table 1. The majority of samples contained at least 1 detectable mycotoxin with co-occurrence (≥ 2 mycotoxins) similar to 2017, and less than 2016. Prevalence of B-Trich has decreased compared with previous years, but average ppb is similar to 2017. Prevalence and average ppb of ZEN are similar to 2017, while FUM has increased in both prevalence and average ppb. Alfa prevalence has increased and average ppb is numerically higher than the previous two years. The preliminary results from the 2018 corn harvest suggest a continued risk from mycotoxins produced by Fusarium fungal species, and a potential increased risk of Afla compared to previous years. Because of the risk of multi-mycotoxin contamination in corn samples thus far, multiple mitigation strategies are needed beyond just adsorption, including biotransformation support of the immune system and liver function.
169 Susceptibility of several species of gram-negative and gram-positive bacteria to organic acids and their derivatives
Authors: Kovanda Lauren , Li Xunde , Zhang Wen , Wei Xiaohong , Luo Jia , Wu Xixi , Atwill Edward Robert , Sygall Richard , Lum Jacob , Liu Yanhong
Published: July 2019

The objective of this experiment was to determine the in vitro antimicrobial activity of several organic acids and their derivatives against gram-positive (G+) and gram-negative (G-) bacteria. Monopropionin, monovalerin, monolaurin, and sodium formate were tested at 10 to 12 concentrations from 0.001 to 2.50 mg/L; monobutyrin was tested at 16 concentrations from 0.001 to 5.00 mg/L; butyric acid and valeric acid were tested at 8 concentrations from 0.001 to 0.35 mg/L; while formic acid was tested at 8 concentrations from 0.001 to 1.00 mg/L. The tested bacteria included G- bacteria (Escherichia coli, Salmonella typhimurium, and Campylobacter jejuni) and G+ bacteria (Enterococcus faecalis, Clostridia perfringens, Streptococcus pneumonia, and Streptococcus suis). Antimicrobial activity of tested compounds was expressed as minimum inhibitory concentration (MIC) that prevented growth of tested bacteria in treated culture broth. Butyric acid, valeric acid, and formic acid at highest tested concentrations inhibited the growth of all bacterial strains. The MICs of the three acids varied among bacterial strains with lowest MIC of 0.05–0.10 mg/L on two strains of Campylobacter. Sodium formate at highest tested concentrations did not inhibit the growth of E. coli, S. typhimurium, and E. faecalis but the compound inhibited the growth of other tested bacteria with MIC values from 0.20 to 1.88 mg/L. The MIC values of monovalerin, monolaurin, and monobutyrin ranged between 0.001 and 1.50 mg/L on all bacterial strains with the exception that monobutyrin did not inhibit C. jejuni (ATCC 33560) growth at the highest concentration tested (5.0 mg/L). Monopropionin at tested concentrations did not inhibit the growth of all tested bacteria with the exception of 1.13 mg/L as MIC on C. perfringens (ATCC 12915). The MIC tests indicate that all organic acids and their derivatives, except monopropionin, tested in this experiment exhibited strong antimicrobial effects in vitro against tested bacterial strains.
185 Impact of hydroxy-selenomethionine supplementation during late gestation and lactation in sows on piglet immunocompetence
Authors: Li Ningya , Sun Zhijian , Karrow Niel A , Briens Mickael , De Marco Michele , Liu Huazhen
Published: July 2019

The benefits of selenium (Se) supplementation during pregnancy and lactation in sows on piglet immunocompetence are not yet fully understood. This study aimed to assess piglet immune status when sows were fed Se-deficient or supplemented diets with sodium selenite (SS) or hydroxy-selenomethionine (OH-SeMet). Twelve pregnant sows were randomly assigned to 3 dietary treatments (4 sows each): no supplemental Se (NC), SS at 0.3 mg Se/kg (SS-0.3), OH-SeMet at 0.3 mg Se/kg (SO-0.3). The trial was carried out from gestation day (gd) 84 to weaning on postpartum day (ppd) 21, at which time all the piglets received the same diet having as Se source SS. On gd 98 and 105, sows were vaccinated with hen egg white lysozyme (HEWL). On ppd 0 and 21, 8 piglets/treatment were euthanized to obtain blood and muscle samples for total Se analyses. Serum samples were collected from 8 female piglets/treatment on ppd 0 and 2 to assess antigen-specific antibody titer for HEWL. On ppd 23, 6 weaned female piglets/treatment were intramuscular challenged with E. coli lipopolysaccharide (LPS) and serum IL-10 levels were measured at 24 h pre-challenge and at 2 and 48 h post challenge. At day 21, piglets from sows fed SO-0.3 showed the highest muscle Se deposition (P < 0.001). At ppd 0, no difference for HEWL-specific IgG was found while at ppd 2, SO-0.3 showed the highest HEWL-specific IgG concentration (P < 0.05). At 24 h pre-challenge and 2 h post LPS challenge, SO-0.3 showed a greater IL-10 level as compare to NC and SS-0.3, respectively (P < 0.05); no difference was found for the same parameter at 48 h post challenge. Piglets from SO supplemented sows exhibited stronger early immune response to LPS challenge. This study indicates the importance of sow Se supplementation with a pure organic form of Se such as OH-SeMet to enhance piglets Se and immune status.
188 Effects of an antioxidant support technology and vitamin E on growth performance of naturally health-challenged nursery pigs
Authors: Ebarb Sara , Saddoris-Clemons Kari , Price Kathryn , Jolliff James , Williams Sabrina
Published: July 2019

The effects of an antioxidant support technology (AOX, Promote® AOX™; Provimi, Brookville, OH) in diets with different levels of vitamin E (VIT-E) were evaluated. A total of 1,848 naturally health-challenged (determined by ~2% higher than the yearly average barn mortality) pigs (~19 d of age; 5.9 ± 0.4 kg), 14 pens/treatment, and 22 pigs/pen were housed in two barns which were blocked separately by pen location and randomly allocated to treatments. Treatments were arranged as a 2 × 3 factorial with three levels of AOX (0, 0.02, 0.13%) and two levels of VIT-E (35 or 100 IU/kg). Pen weights were obtained on d 0, 7, 21, and 40 post-weaning. Growth performance and mortalities and removals were evaluated. Constructed contrasts were: 0 vs additional AOX (aggregate effect of 0.02 and 0.13 AOX), 0.02 vs 0.13 AOX, 35 vs 100 VIT-E, and all possible interactions. Overall (d 0 to 40), interactions were observed (P < 0.05) between additional AOX and VIT-E level for d 40 BW (35 VIT-E: 22.0, 22.3, 22.8 kg; 100 VIT-E: 23.0, 22.8, 22.3 kg), ADG (35 VIT-E: 0.39, 0.40, 0.42 kg/d; 100 VIT-E: 0.42, 0.41, 0.40 kg/d), ADFI (35 VIT-E: 0.52, 0.52, 0.54 kg/d; 100 VIT-E: 0.55, 0.54, 0.52 kg/d, and return over feed cost (ROFC; 35 VIT-E: $27.16, 27.62, 28.29; 100 VIT-E: $28.83, 28.31, 27.50). Additionally, interactions were observed (P < 0.05) between AOX level, when increased from 0.02 to 0.13%, and VIT-E level for ADG, ADFI, ROFC, and probability for mortality and removals (35 VIT-E: 10.4, 13.6, 6.5%; 100 VIT-E: 8.4, 10.0, 9.7%). Level of AOX or VIT-E did not impact cost/kg of gain or feed efficiency (P > 0.10). Including AOX in 35 IU/kg VIT-E diets improved growth performance parameters and reduced probability for mortality and removals, but not in diets containing 100 IU/kg VIT-E.