bluuebunny/crossref_metadata_2025 huggingface dataset:
Repellent and Antifeedant Activities of Citral-Derived Lactones against the Peach Potato Aphid
Authors: Dancewicz Katarzyna , Szumny Antoni , Wawrzeńczyk Czesław , Gabryś Beata
Published: October 2020

Citral is well known for its antimicrobial, antifungal, and insecticidal activities. Natural sesquiterpene α-methylenelactones also exhibit a broad spectrum of biological activities. The aim of the study was to explore the effect of structural changes to citral molecules on citral behavior-modifying activity towards Myzus persicae. Specifically, the effects of the introduction of a γ-lactone moiety and methylene groups in α and γ positions of the lactone ring were investigated. The lactones were obtained in five-step (saturated lactone and γ-methylenelactone) or six-step (α-methylenelactone and α,γ-dimethylenelactone) syntheses from citral. The synthetic procedures and physical and spectral data of the lactones are presented. The settling behavior of freely moving aphids in choice and no-choice situations was monitored. The probing behavior of tethered M. persicae using the Electrical Penetration Graph (EPG) technique was also analyzed. Citral appeared a strong repellent and pre-ingestive and ingestive probing deterrent to M. persicae. The incorporation of a lactone moiety caused the loss of the repellent activity. α-Methylenelactone inhibited aphid settling and probing activities at pre-ingestive and ingestive phases. The saturated γ-lactone and α,γ-dimethylenelactone were the settling post-ingestive deterrents to M. persicae, which did not affect aphid probing activity. γ-Methylenelactone did not affect aphid behavior.
Cannabinoids Inhibited Pancreatic Cancer via P-21 Activated Kinase 1 Mediated Pathway
Authors: Yang Yang , Huynh Nhi , Dumesny Chelsea , Wang Kai , He Hong , Nikfarjam Mehrdad
Published: October 2020

The anti-cancer effects of cannabinoids including CBD (Cannabidiol) and THC ((−)-trans-∆9-tetrahydrocannabinol) have been reported in the case of pancreatic cancer (PC). The connection of these cannabinoids to KRas oncogenes that mutate in more than 90% of PC, and their effects on PD-L1, a key target of immune checkpoint blockade, have not been thoroughly investigated. Using cell lines and mouse models of PC, the effects of CBD and THC on cancer growth, the interaction between PC cells and a stromal cell, namely pancreatic stellate cells (PSCs), and the mechanism(s) involved were determined by cell-based assays and mouse study in vivo. CBD and THC inhibited the proliferation of PC, PSC, and PSC-stimulated PC cells. They also suppressed pancreatic tumour growth in mice. Furthermore, CBD and/or THC reduced the expression of PD-L1 by either PC or PSC cells. Knockout of p-21 activated kinase 1 (PAK1, activated by KRas) in PC and PSC cells and, in mice, dramatically decreased or blocked these inhibitory effects of CBD and/or THC. These results indicated that CBD and THC exerted their inhibitions on PC and PSC via a p-21 activated kinase 1 (PAK1)-dependent pathway, suggesting that CBD and THC suppress Kras activated pathway by targeting PAK1. The inhibition by CBD and THC of PD-L1 expression will enhance the immune checkpoint blockade of PC.
A Resilience-Based Methodology to Assess Soil Structure Interaction on a Benchmark Bridge
Authors: Forcellini Davide
Published: October 2020

The assessment of bridge functionality during earthquakes is fundamental in the evaluation of emergency response and socio-economic recovery procedures. In this regard, resilience may be considered a key parameter for decision-making procedures such as post-hazard event mitigations and recovery investments on bridges. The paper proposes a case study of a bridge configuration subjected to seismic hazard and aims to consider the effects of the soil–structure interaction on the recovery to various levels of pre-earthquake functionality. The principal outcome of the paper consists of calculating resilience as a readable finding that may have many applications for a wide range of stakeholders, such as bridge owners, transportation authorities and public administrators who can apply the outcomes in the assessment of the best recovery techniques and solutions.
Lethal Doses of Saponins from Quillaja saponaria for Invasive Slug Arion vulgaris and Non-Target Organism Enchytraeus albidus (Olygochaeta: Enchytraeidae)
Authors: Adomaitis Mantas , Skujienė Grita
Published: October 2020

The slug, Arion vulgaris Moquin-Tandon, 1855, is a serious pest in agriculture and private gardens. White worm, Enchytraeus albidus Henle, 1837, is an important model of decomposer organism in the terrestrial ecosystem. Saponins, which are secondary metabolites of plants, have previously been shown to have some molluscicidal effect. We investigated which doses of saponins are lethal to the slug, A. vulgaris, and to the non-target organism, E. albidus. An aqueous solution with different concentrations of saponin extract from the bark of the soap tree, Quillaja saponaria Mol., was used in repeat treatments. Slugs were tested in filter paper contact tests as they are naturally exposed to soil contact while crawling. Worms were tested in soil contact tests as they live below ground. It was found that lethality of saponins depends on the slug age group and the environmental temperature. The median lethal concentration (LC50, at 15 °C) on adults was 68.5 g/L, and on juveniles, 96.9 g/L. The slugs were significantly more sensitive at 2 and −1 °C compared to 15 °C. The LC50 (at 6 ℃) on E. albidus was 2.7 g/L (or 0.5 g/kg dry weight of soil), far below those in A. vulgaris (at 15 ℃ and lower). The LC50 for worms at -1℃ was also significantly lower than at 6 ℃. Therefore, we can conclude: (1) that Q. saponaria saponins may be a successful slug control tool used during colder times of the year, but its concentration should be selected according to the age group of A. vulgaris; (2) this measure is more toxic than expected to white worms, which limits its use.
Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae)
Authors: Arora Arinder K. , Clark Noah , Wentworth Karen S. , Hesler Stephen , Fuchs Marc , Loeb Greg , Douglas Angela E.
Published: October 2020

The grape mealybug Pseudococcus maritimus (Ehrhorn, 1900) (Hemiptera: Pseudococcidae) is a significant pest of grapevines (Vitis spp.) and a vector of disease-causing grape viruses, linked to its feeding on phloem sap. The management of this pest is constrained by the lack of naturally occurring resistance traits in Vitis. Here, we obtained proof of concept that RNA interference (RNAi) using double-stranded RNA (dsRNA) molecules against essential genes for phloem sap feeding can depress insect survival. The genes of interest code for an aquaporin (AQP) and a sucrase (SUC) that are required for osmoregulation in related phloem sap-feeding hemipteran insects (aphids and whiteflies). In parallel, we investigated the grape mealybug genes coding non-specific nucleases (NUC), which reduce RNAi efficacy by degrading administered dsRNA. Homologs of AQP and SUC with experimentally validated function in aphids, together with NUC, were identified in the published transcriptome of the citrus mealybug Planococcus citri by phylogenetic analysis, and sequences of the candidate genes were obtained for Ps. maritimus by PCR with degenerate primers. Using this first sequence information for Ps. maritimus, dsRNA was prepared and administered to the insects via an artificial diet. The treatment comprising dsRNA against AQP, SUC and NUC significantly increased insect mortality over three days, relative to dsRNA-free controls. The dsRNA constructs for AQP and NUC were predicted, from sequence analysis to have some activity against other mealybugs, but none of the three dsRNA constructs have predicted activity against aphids. This study provides the basis to develop in planta RNAi strategies against Ps. maritimus and other mealybug pests of grapevines.
Physicochemical Characteristics of Four Limonene-Based Nanoemulsions and Their Larvicidal Properties against Two Mosquito Species, Aedes albopictus and Culex pipiens molestus
Authors: Theochari Ioanna , Giatropoulos Athanasios , Papadimitriou Vassiliki , Karras Vasileios , Balatsos Georgios , Papachristos Dimitrios , Michaelakis Antonios
Published: October 2020

Negative impacts on the environment from the continuous use of synthetic insecticides against mosquitoes has driven research towards more ecofriendly products. Phytochemicals, classified as low-risk substances, have been recognized as potential larvicides of mosquitoes; however, problems related to water solubility and stability are limiting factors for their use in mosquito control programs in the field. In this context, many researchers have focused on formulating essential oils in nanoemulsions, exploiting innovative nanotechnology. In the current study, we prepared 4 (R)-(+)-limonene oil-in-water nanoemulsions using low and high energy methods, and we evaluated their physicochemical characteristics (e.g., viscosity, stability, mean droplet diameter, polydispersity index) and their bioactivity against larvae of two mosquito species of great medical importance, namely, Cx. pipiens molestus and Ae. albopictus. According to the dose–response bioassays with the limonene-based nanoemulsions and pure limonene (dissolved in organic solvent), the tested nanoformulations improved the activity of limonene against Ae. albopictus larvae, while the performance of limonene was either the same or better than limonene against Cx. pipiens molestus, depending on the applied system. Overall, we achieved the production of limonene-based delivery nanosystems, with sufficient lethal properties against mosquito larvae to consider them promising larvicidal formulations applicable to mosquito breeding sites.
Towards Real-Time Reinforcement Learning Control of a Wave Energy Converter
Authors: Anderlini Enrico , Husain Salman , Parker Gordon G. , Abusara Mohammad , Thomas Giles
Published: October 2020

The levellised cost of energy of wave energy converters (WECs) is not competitive with fossil fuel-powered stations yet. To improve the feasibility of wave energy, it is necessary to develop effective control strategies that maximise energy absorption in mild sea states, whilst limiting motions in high waves. Due to their model-based nature, state-of-the-art control schemes struggle to deal with model uncertainties, adapt to changes in the system dynamics with time, and provide real-time centralised control for large arrays of WECs. Here, an alternative solution is introduced to address these challenges, applying deep reinforcement learning (DRL) to the control of WECs for the first time. A DRL agent is initialised from data collected in multiple sea states under linear model predictive control in a linear simulation environment. The agent outperforms model predictive control for high wave heights and periods, but suffers close to the resonant period of the WEC. The computational cost at deployment time of DRL is also much lower by diverting the computational effort from deployment time to training. This provides confidence in the application of DRL to large arrays of WECs, enabling economies of scale. Additionally, model-free reinforcement learning can autonomously adapt to changes in the system dynamics, enabling fault-tolerant control.
Comparison of β-D-Glucan and Galactomannan in Serum for Detection of Invasive Aspergillosis: Retrospective Analysis with Focus on Early Diagnosis
Authors: Dichtl Karl , Forster Johannes , Ormanns Steffen , Horns Heidi , Suerbaum Sebastian , Seybold Ulrich , Wagener Johannes
Published: October 2020

The early diagnosis of invasive aspergillosis (IA) relies mainly on computed tomography imaging and testing for fungal biomarkers such as galactomannan (GM). We compared an established ELISA for the detection of GM with a turbidimetric assay for detection of the panfungal biomarker β-D-glucan (BDG) for early diagnosis of IA. A total of 226 serum specimens from 47 proven and seven probable IA cases were analysed. Sensitivity was calculated for samples obtained closest to the day of IA-diagnosis (d0). Additional analyses were performed by including samples obtained during the presumed course of disease. Most IA cases involved the respiratory system (63%), and Aspergillus fumigatus was the most frequently isolated species (59%). For proven cases, sensitivity of BDG/GM analysis was 57%/40%. Including all samples dating from –6 to +1 weeks from d0 increased sensitivities to 74%/51%. Sensitivity of BDG testing was as high as or higher than GM testing for all subgroups and time intervals analysed. BDG testing was less specific (90–93%) than GM testing (99–100%). Combining BDG and GM testing resulted in sensitivity/specificity of 70%/91%. Often, BDG testing was positive before GM testing. Our study backs the use of BDG for diagnosis of suspected IA. We suggest combining BDG and GM to improve the overall sensitivity.
The Changing Dynamics of Board Independence: A Copula Based Quantile Regression Approach
Authors: Kim Jong-Min , Cho Chanho , Jun Chulhee , Kim Won Yong
Published: October 2020

This paper examines the effect of board characteristics, especially board independence, on firm performance from a dynamic perspective through copula-based quantile regression approaches, which allow us to focus on changes at different points in the distribution of board characteristics. We find that the effect of board independence on Tobin’s Q, a proxy of firm value, is negatively associated with firm value, using ordinary least squares (OLS) regression. This negative effect using the conditional mean of the firm value does not hold across the conditional quantiles of the distribution of Tobin’s Q, and this finding is still held under both the linear and the nonlinear quantile regressions. We even lessen the assumption of distributions of multivariate board variables by employing parametric copula-based quantile regressions as well as nonparametric ones. The results support our findings. Our results suggest that estimating the quantile effect of board variables on firm value can provide more meaningful insight than just examining the conditional mean effect.
Combining Participatory Processes and Sustainable Development Goals to Revitalize a Rural Area in Cantabria (Spain)
Authors: Diaz-Sarachaga Jose Manuel
Published: October 2020

Due to industrial civilization, the decline of the countryside has become a global phenomenon. Spain is a good example that reflects this issue in the rural areas of the European Union because more than half of all municipalities in the country are at risk of extinction. This manuscript aims at combining social engagement and the application of sustainable development goals (SDGs) to propose a sustainable rural revitalization plan. Several multi-sectoral working groups participated in three consultation rounds to prescribe 52 customized actions directed to Pesquera-San Miguel de Aguayo area. Social (17) and environmental (16) dimensions collected the highest number of initiatives, whilst the institutional dimension was only addressed by eight proposals. Furthermore, 40 out of the 169 SDG targets of the 2030 Agenda were put forward in the rural strategy, which also contemplated most priorities adopted by the Rural Development Programme for Cantabria. A cooperative association was identified as the most preferred governance system in order to build a community spirit and promote social empowerment, inclusiveness, and gender equality. Municipal and regional authorities were not invited to join the participatory process in order to not constrain the involvement of the selected stakeholders.
Green Infrastructure Planning in Metropolitan Regions to Improve the Connectivity of Agricultural Landscapes and Food Security
Authors: Yacamán Ochoa Carolina , Ferrer Jiménez Daniel , Mata Olmo Rafael
Published: October 2020

Green infrastructure (GI), as a concept and as a tool for environmental land-use planning at various scales, has burst onto the academic, political, and policy-making scenes in the last two decades. This tool, associated with strategic planning, offers integrated solutions for improving the ecological connectivity and urban resilience of open spaces, especially those affected by processes of urban sprawl, the abandonment of agriculture, and the territorial fragmentation of habitats and traditional agricultural landscapes. In spite of the advantages of GI, its design and implementation face a range of challenges and limitations. In this context, this paper has two objectives: Firstly, to address a critical review of recent literature on the subject, which, among other things, highlights the lack of references to the role of peri-urban agriculture in GI planning, and the positive contribution made by peri-urban agriculture to the local food supply and other regulatory and cultural services. Secondly, to propose a methodology to contribute to integrating practical GI planning in metropolitan regions to maximize the activation of traditional agricultural landscapes and the improvement of landscape connectivity in metropolitan regions for the reconnection of rural-urban relationships.
When Tradition Meets Modernity in Land Registration: Evidence from Dagbon, Ghana
Authors: Edwin David Asante , Glover Evam Kofi , Glover Edinam K.
Published: October 2020

Development practice over recent years in much of Africa prioritized formalization of land policies deemed to enhance better handling and use of land as an asset for social development. Following this trend, land reform policy in Ghana was based on a pluralistic legal system in which both the customary land tenure system and the statutory system of land ownership and control co-exist by law. The primary research question for this study was the following: What implications emerge when customary land tenure system and the statutory system of land ownership and control co-exist in law? The study discussed the prospects and challenges of land title registration and the meaning of the new organizing concept in land ownership and administration among the people of Dagbon in the northern region of Ghana. The principal aim of the study was to assess the challenges of the implementation of a modern land registration system over a deeply traditional one. A qualitative research methodology was used and included qualitative descriptive analysis. This descriptive-analytical study was carried out to investigate opinions on the implications of the merger and preferred options for redress of any systemic challenges. It employed Focused Group Discussions (FGDs) to supplement in-depth interviews. Interviews were conducted among 40 key participants within formal and informal institutions including officials from both the Land Commission and Town and Country Planning Departments. Purposeful sampling was employed, and an interview guide was developed and used for collecting the data. Data were analyzed using a thematic approach. The results showed that in this structural reform, the ‘allodial title’ holder was much more trusted for tenure security because of the traditional legitimacy of the King as the sole owner and controller of land. The title registration system therefore principally served the secondary purpose as additional security. The findings indicate that in the circumstance where the law was seen as pliable, the policy engendered blurred and confusing effects that deepened the sense of ambiguity and outcomes were sometimes contradictory. We argued that the crossroads presented challenges that were novel and engendered innovative thinking for more appropriate solutions. The study revealed that policy reforms must be tailor-made to the physical, social, cultural and economic settings.
Connexin Genes Variants Associated with Non-Syndromic Hearing Impairment: A Systematic Review of the Global Burden
Authors: Adadey Samuel Mawuli , Wonkam-Tingang Edmond , Twumasi Aboagye Elvis , Nayo-Gyan Daniel Wonder , Boatemaa Ansong Maame , Quaye Osbourne , Awandare Gordon A. , Wonkam Ambroise
Published: October 2020

Mutations in connexins are the most common causes of hearing impairment (HI) in many populations. Our aim was to review the global burden of pathogenic and likely pathogenic (PLP) variants in connexin genes associated with HI. We conducted a systematic review of the literature based on targeted inclusion/exclusion criteria of publications from 1997 to 2020. The databases used were PubMed, Scopus, Africa-Wide Information, and Web of Science. The protocol was registered on PROSPERO, the International Prospective Register of Systematic Reviews, with the registration number “CRD42020169697”. The data extracted were analyzed using Microsoft Excel and SPSS version 25 (IBM, Armonk, New York, United States). A total of 571 independent studies were retrieved and considered for data extraction with the majority of studies (47.8% (n = 289)) done in Asia. Targeted sequencing was found to be the most common technique used in investigating connexin gene mutations. We identified seven connexin genes that were associated with HI, and GJB2 (520/571 publications) was the most studied among the seven. Excluding PLP in GJB2, GJB6, and GJA1 the other connexin gene variants (thus GJB3, GJB4, GJC3, and GJC1 variants) had conflicting association with HI. Biallelic GJB2 PLP variants were the most common and widespread variants associated with non-syndromic hearing impairment (NSHI) in different global populations but absent in most African populations. The most common GJB2 alleles found to be predominant in specific populations include; p.Gly12ValfsTer2 in Europeans, North Africans, Brazilians, and Americans; p.V37I and p.L79Cfs in Asians; p.W24X in Indians; p.L56Rfs in Americans; and the founder mutation p.R143W in Africans from Ghana, or with putative Ghanaian ancestry. The present review suggests that only GJB2 and GJB3 are recognized and validated HI genes. The findings call for an extensive investigation of the other connexin genes in many populations to elucidate their contributions to HI, in order to improve gene-disease pair curations, globally.
Eco-Friendly Water-Based Nanolubricants for Industrial-Scale Hot Steel Rolling
Authors: Wu Hui , Kamali Hamidreza , Huo Mingshuai , Lin Fei , Huang Shuiquan , Huang Han , Jiao Sihai , Xing Zhao , Jiang Zhengyi
Published: October 2020

Eco-friendly and low-cost water-based nanolubricants containing rutile TiO2 nanoparticles (NPs) were developed for accelerating their applications in industrial-scale hot steel rolling. The lubrication performance of developed nanolubricants was evaluated in a 2-high Hille 100 experimental rolling mill at a rolling temperature of 850 °C in comparison to that of pure water. The results indicate that the use of nanolubricant enables one to decrease the rolling force, reduce the surface roughness and the oxide scale thickness, and enhance the surface hardness. In particular, the nanolubricant consisting of 4 wt % TiO2, 10 wt % glycerol, 0.2 wt % sodium dodecyl benzene sulfonate (SDBS) and 1 wt % Snailcool exhibits the best lubrication performance by lowering the rolling force, surface roughness and oxide scale thickness by up to 8.1%, 53.7% and 50%, respectively. The surface hardness is increased by 4.4%. The corresponding lubrication mechanisms are attributed to its superior wettability and thermal conductivity associated with the synergistic effect of rolling, mending and laminae forming that are contributed by TiO2 NPs.
Experimental and Numerical Study of Vacuum Resin Infusion of Stiffened Carbon Fiber Reinforced Panels
Authors: Lionetto Francesca , Moscatello Anna , Totaro Giuseppe , Raffone Marco , Maffezzoli Alfonso
Published: October 2020

Liquid resin infusion processes are becoming attractive for aeronautic applications as an alternative to conventional autoclave-based processes. They still present several challenges, which can be faced only with an accurate simulation able to optimize the process parameters and to replace traditional time-consuming trial-and-error procedures. This paper presents an experimentally validated model to simulate the resin infusion process of an aeronautical component by accounting for the anisotropic permeability of the reinforcement and the chemophysical and rheological changes in the crosslinking resin. The input parameters of the model have been experimentally determined. The experimental work has been devoted to the study of the curing kinetics and chemorheological behavior of the thermosetting epoxy matrix and to the determination of both the in-plane and out-of-plane permeability of two carbon fiber preforms using an ultrasonic-based method, recently developed by the authors. The numerical simulation of the resin infusion process involved the modeling of the resin flow through the reinforcement, the heat exchange in the part and within the mold, and the crosslinking reaction of the resin. The time necessary to fill the component has been measured by an optical fiber-based equipment and compared with the simulation results.
Mechanical Performance of Unstitched and Silk Fiber-Stitched Woven Kenaf Fiber-Reinforced Epoxy Composites
Authors: Kirmasha Yasir Khaleel , Sharba Mohaiman J. , Leman Zulkiflle , Sultan Mohamed Thariq Hameed
Published: October 2020

Fiber composites are known to have poor through-thickness mechanical properties due to the absence of a Z-direction binder. This issue is more critical with the use of natural fibers due to their low strength compared to synthetic fibers. Stitching is a through-thickness toughening method that is used to introduce fibers in the Z-direction, which will result in better through-thickness mechanical properties. This research was carried out to determine the mechanical properties of unstitched and silk fiber-stitched woven kenaf-reinforced epoxy composites. The woven kenaf mat was stitched with silk fiber using a commercial sewing machine. The specimens were fabricated using a hand lay-up method. Three specimens were fabricated, one unstitched and two silk-stitched with deferent stitching orientations. The results show that the stitched specimens have comparable in-plane mechanical properties to the unstitched specimens. For the tensile mechanical test, stitched specimens show similar and 17.1% higher tensile strength compared to the unstitched specimens. The flexural mechanical test results show around a 9% decrease in the flexural strength for the stitched specimens. On the other hand, the Izod impact mechanical test results show a significant improvement of 33% for the stitched specimens, which means that stitching has successfully improved the out-of-plane mechanical properties. The outcome of this research indicates that the stitched specimens have better mechanical performance compared to the unstitched specimens and that the decrease in the flexural strength is insignificant in contrast with the remarkable enhancement in the impact strength.
Fast and Efficient Piezo/Photocatalytic Removal of Methyl Orange Using SbSI Nanowires
Authors: Mistewicz Krystian , Kępińska Mirosława , Nowak Marian , Sasiela Agnieszka , Zubko Maciej , Stróż Danuta
Published: October 2020

Piezocatalysis is a novel method that can be applied for degradation of organic pollutants in wastewater. In this paper, ferroelectric nanowires of antimony sulfoiodide (SbSI) have been fabricated using a sonochemical method. Methyl orange (MO) was chosen as a typical pollutant, as it is widely used as a dye in industry. An aqueous solution of MO at a concentration of 30 mg/L containing SbSI nanowires (6 g/L) was subjected to ultrasonic vibration. High degradation efficiency of 99.5% was achieved after an extremely short period of ultrasonic irradiation (40 s). The large reaction rate constant of 0.126(8) s−1 was determined for piezocatalytic MO decomposition. This rate constant is two orders of magnitude larger than values of reaction rate constants reported in the literature for the most efficient piezocatalysts. These promising experimental results have proved a great potential of SbSI nanowires for their application in environmental purification and renewable energy conversion.
Physical Characterization of Sintered NiMnGa Ferromagnetic Shape Memory Alloy
Authors: Villa Francesca , Nespoli Adelaide , Fanciulli Carlo , Passaretti Francesca , Villa Elena
Published: October 2020

The present work focused on the microstructural, thermal, electrical, and damping characterization of NiMnGa samples produced through a powder pressing and a sintering process; the effect of sintering times and of the starting powder size were evaluated. Moreover, an observation of the evolution of martensitic transformation typical of NiMnGa ferromagnetic shape memory alloy was conducted in comparison with the cast material behavior and in correlation with the material densification. The optimum powder size and sintering time for the process, i.e., 50 µm or lower and 72 h, were identified considering the investigated physical properties of the sintered samples in comparison to the cast material. The corresponding sample showed the best compromise between density, thermal and electrical properties, and damping and functional behaviour. In general, the outcomes of this study could be the basis of a useful tool for production processes that include a sintering step as well as being a starting point for the evaluation of an alternative low cost fabrication method of this alloy.
3D Cell Culture of Human Salivary Glands Using Nature-Inspired Functional Biomaterials: The Egg Yolk Plasma and Egg White
Authors: Charbonneau André M. , Tran Simon D.
Published: October 2020

The egg yolk plasma (EYP)—a translucent fraction of the egg yolk (EY) obtained by centrifugation—was tested as a developmentally encouraging, cost-effective, biomaterial for salivary gland (SG) tissue engineering. To find optimal incubating conditions for both the human NS-SV-AC SG acinar cell line and SG fibroblasts, cells were stained with Live/Dead®. The cellular contents of 96-well plates were analyzed by high content screening image analysis. Characteristically, the EYP biomaterial had lipid and protein content resembling the EY. On its own, the EYP was non-conducive to cell survival. EYP’s pH of 6 mainly contributed to cell death. This was demonstrated by titrating EYP’s pH with different concentrations of either commercial cell culture media, NaOH, or egg white (EW). These additives improved SG mesenchymal and epithelial cell survival. The best combinations were EYP diluted with (1) 70% commercial medium, (2) 0.02 M NaOH, or (3) 50% EW. Importantly, commercial medium-free growth was obtained with EYP + NaOH or EYP + EW. Furthermore, 3D cultures were obtained as a result of EW’s gelatinous properties. Here, the isolation, characterization, and optimization of three EYP-based biomaterial combinations are shown; two were free of commercial medium or supplements and supported both SG cells’ survival.
Melt-Spinning of an Intrinsically Flame-Retardant Polyacrylonitrile Copolymer
Authors: König Simon , Kreis Philipp , Herbert Christian , Wego Andreas , Steinmann Mark , Wang Dongren , Frank Erik , Buchmeiser Michael
Published: October 2020

Poly(acrylonitrile) (PAN) fibers have two essential drawbacks: they are usually processed by solution-spinning, which is inferior to melt spinning in terms of productivity and costs, and they are flammable in air. Here, we report on the synthesis and melt-spinning of an intrinsically flame-retardant PAN-copolymer with phosphorus-containing dimethylphosphonomethyl acrylate (DPA) as primary comonomer. Furthermore, the copolymerization parameters of the aqueous suspension polymerization of acrylonitrile (AN) and DPA were determined applying both the Fineman and Ross and Kelen and Tüdõs methods. For flame retardancy and melt-spinning tests, multiple PAN copolymers with different amounts of DPA and, in some cases, methyl acrylate (MA) have been synthesized. One of the synthesized PAN-copolymers has been melt-spun with propylene carbonate (PC) as plasticizer; the resulting PAN-fibers had a tenacity of 195 ± 40 MPa and a Young’s modulus of 5.2 ± 0.7 GPa. The flame-retardant properties have been determined by Limiting Oxygen Index (LOI) flame tests. The LOI value of the melt-spinnable PAN was 25.1; it therefore meets the flame retardancy criteria for many applications. In short, the reported method shows that the disadvantage of high comonomer content necessary for flame retardation can be turned into an advantage by enabling melt spinning.
Production of Soda Lime Glass Having Antibacterial Property for Industrial Applications
Authors: Demirel Barış , Erol Taygun Melek
Published: October 2020

This study was aimed to produce and characterize the first commercial glass materials with enhanced antibacterial property using conventional melting method. For this purpose, typical container glass composition that contains some specific metal ions, such as silver, strontium, and copper, was used to obtain antibacterial glass samples using classical melting method. After the melting process, antibacterial tests and migration tests were applied to the glasses, and it was found that the glass doped with 2% Ag2O was the best composition. X-rays diffractometer (XRD), thermal expansion coefficient, density, refractive index, hardness, and elastic module results showed that the glass doped with 2% Ag2O was a suitable material as a container glass. High Temperature Melting Observation System studies were performed on the produced antibacterial glass composition, and it was found that the antibacterial glass can be produced in soda lime glass furnaces without changing any furnace design and production parameters. As a result of the characterization studies, it was concluded that the produced container glass doped with silver can be a good candidate for food and pharmaceutical products where bacterial growth is absolutely undesirable.
Phototherapy Combined with Carbon Nanomaterials (1D and 2D) and Their Applications in Cancer Therapy
Authors: Sundaram Prabhavathi , Abrahamse Heidi
Published: October 2020

Carbon-based materials have attracted research interest worldwide due to their physical and chemical properties and wide surface area, rendering them excellent carrier molecules. They are widely used in biological applications like antimicrobial activity, cancer diagnosis, bio-imaging, targeting, drug delivery, biosensors, tissue engineering, dental care, and skin care. Carbon-based nanomaterials like carbon nanotubes and graphene have drawn more attention in the field of phototherapy due to their unique properties such as thermal conductivity, large surface area, and electrical properties. Phototherapy is a promising next-generation therapeutic modality for many modern medical conditions that include cancer diagnosis, targeting, and treatment. Phototherapy involves the major administration of photosensitizers (PSs), which absorb light sources and emit reactive oxygen species under cellular environments. Several types of nontoxic PSs are functionalized on carbon-based nanomaterials and have numerous advantages in cancer therapy. In this review, we discuss the potential role and combined effect of phototherapy and carbon nanomaterials, the mechanism and functionalization of PSs on nanomaterials, and their promising advantages in cancer therapy.
Numerical Simulations to Predict the Seismic Performance of a 2-Story Steel Moment-Resisting Frame
Authors: Tartaglia Roberto , D’Aniello Mario , Landolfo Raffaele
Published: October 2020

The seismic response of steel moment resisting frames (MRFs) is influenced by the behavior of joints. Within the ongoing research project “FUTURE”(Full-scale experimental validation of steel moment frame with EU qualified joints and energy efficient claddings under Near fault seismic scenarios), shake table tests will be carried out on a two-story one bay MRF equipped with different types of prequalified beam-to-column joints. In order to design the experimental campaign, preliminary numerical simulations have been carried out to predict the seismic performance of the experimental mock-up in terms of distribution of damage, transient and residual interstory drifts. In this paper the main modeling assumptions and the results of the seismic analyses are shown and discussed. In particular, the response of joints was systematically investigated by refined finite element (FE) simulations and their behavior was taken into account in the global structural performance by means of both concentrated plastic hinge and distributed plasticity models. Both static and dynamic non-linear analyses show in which terms the type of models for plastic hinges influences the results. The modeling approach plays a key role only at very high seismic intensity where large ductility demand is imposed. In addition, changing the type of joints has less influence on the overall response of the frame.
Wire Electrical Discharge Machining—A Review
Authors: Slătineanu Laurenţiu , Dodun Oana , Coteaţă Margareta , Nagîţ Gheorghe , Băncescu Irina Beşliu , Hriţuc Adelina
Published: October 2020

Wire electrical discharge machining has appeared mainly in response to the need for detachment with sufficiently high accuracy of parts of plate-type workpieces. The improvements introduced later allowed the extension of this machining technology to obtain more complex ruled surfaces with increasingly high requirements regarding the quality of the machined surfaces and the productivity of the wire electrical discharge machining process. Therefore, it was normal for researchers to be interested in developing more and more in-depth investigations into the various aspects of wire electrical discharge machining. These studies focused first on improving the machining equipment, wire electrodes, and the devices used to position the clamping of a wire electrode and workpiece. A second objective pursued was determining the most suitable conditions for developing the machining process for certain proper situations. As output parameters, the machining productivity, the accuracy, and roughness of the machined surfaces, the wear of the wire electrode, and the changes generated in the surface layer obtained by machining were taken into account. There is a large number of scientific papers that have addressed issues related to wire electrical discharge machining. The authors aimed to reveal the aspects that characterize the process, phenomena, performances, and evolution trends specific to the wire electrical discharge machining processes, as they result from scientific works published mainly in the last two decades.
Changes in Bond Strength and Topography for Y-TZP Etched with Hydrofluoric Acid Depending on Concentration and Temperature Conditions
Authors: Kim Hyo-Eun , Lim Myung-Jin , Yu Mi-Kyung , Lee Kwang-Won
Published: October 2020

Background and objectives: This study aimed to investigate the change in bond strength between resin cement and tetragonal zirconia polycrystalline stabilized with 3 to 8 mol% yttrium oxide (Y-TZP) and observe the topographical change of the Y-TZP surface when etched with hydrofluoric acid (HF) solution under different concentration and temperature conditions. Materials and Methods: Non-etched sintered Y-TZP specimens under two different temperature conditions (room temperature and 70–80 °C, respectively), were used as a control, while experimental groups were etched with 5%, 10%, 20%, and 40% HF solutions for 10 min. After zirconia primer and MDP-containing resin cement were applied to the Y-TZP surface, the shear bond strength (SBS) of each experimental group was measured. Results: Under room temperature conditions, the highest SBS value was measured in the 40% HF etching group, representing a significant deviation from the other groups (p < 0.05). In the 70–80 °C tests, the 40% HF etching group also had the highest SBS value, but there was no significant difference when compared to the 20% HF etching group (p > 0.05). From SEM and AFM observations, the HF solution increasingly dissolved the Y-TZP surface grain structure as the concentration and application temperature rose, resulting in high surface roughness and irregularities. Conclusions: Pretreating with either 20% HF solution at 70–80 °C or 40% HF solution at room temperature and 70–80 °C effectively acid etched the Y-TZP surface, resulting in more surface roughness and irregularities. Accounting for the concentration and temperature conditions of the HF solution, using 40% HF solution at room temperature will result in improvements in adhesion between resin cement and Y-TZP.
Use of Herbal Medications for Treatment of Osteoarthritis and Rheumatoid Arthritis
Authors: Lindler Breanna N. , Long Katelyn E. , Taylor Nancy A. , Lei Wei
Published: October 2020

Arthritis is a chronic condition that affects nearly a quarter of the United States population. Osteoarthritis (OA) and rheumatoid arthritis (RA) are two major forms of arthritis associated with severe joint pain and reduced quality of life. Various pharmacological interventions may be utilized for arthritis treatment when non-pharmacological therapy is insufficient. However, pharmacological therapy can be associated with serious side effects and high costs. Therefore, alternative therapies have been under investigation. Herbal medications have shown the potential for safe and effective management of arthritis. For this review, we attempt to summarize the mechanisms, safety, and efficacy of herbal treatments for OA and RA. After searching electronic databases, we identified nine herbs among 23 clinical trials used for the treatment of OA or RA patients. Improvement of OA and RA symptoms, pain, and inflammation was demonstrated. The herbs exhibited strong anti-inflammatory and anti-oxidant activities, contributing to a reduction in inflammation and tissue damage. Several herbs elucidated new mechanisms for OA and RA treatment as well. Though these herbs have shown promise for OA and RA treatment, more studies and clinical trials are required for determining safety and efficacy, bioactivity, and optimal bioavailability.
UV-Polymerized Vinylimidazolium Ionic Liquids for Permselective Membranes
Authors: Sommer Fridolin O. , Appelt Jana-Sophie , Barke Ingo , Speller Sylvia , Kragl Udo
Published: October 2020

Ionic liquids are highly charged compounds with increasing applications in material science. A universal approach to synthesize free-standing, vinylalkylimidazolium bromide-containing membranes with an adjustable thickness is presented. By the variation of alkyl side chains, membrane characteristics such as flux and mechanical properties can be adjusted. The simultaneous use of different ionic liquids (ILs) in the synthesis can also improve the membrane properties. In separation application, these charged materials allowed us to retain charged sugars, such as calcium gluconate, by up to 95%, while similar neutral compounds such as glucose passed the membrane. An analysis of the surface conditions using atomic force microscopy (AFM) confirmed the experimental data and explains the decreasing permeance and increased retention of the charged sugars.
Method for Passive Droplet Sorting after Photo-Tagging
Authors: Dobson Chandler , Zielke Claudia , Pan Ching , Feit Cameron , Abbyad Paul
Published: October 2020

We present a method to photo-tag individual microfluidic droplets for latter selection by passive sorting. The use of a specific surfactant leads to the interfacial tension to be very sensitive to droplet pH. The photoexcitation of droplets containing a photoacid, pyranine, leads to a decrease in droplet pH. The concurrent increase in droplet interfacial tension enables the passive selection of irradiated droplets. The technique is used to select individual droplets within a droplet array as illuminated droplets remain in the wells while other droplets are eluted by the flow of the external oil. This method was used to select droplets in an array containing cells at a specific stage of apoptosis. The technique is also adaptable to continuous-flow sorting. By passing confined droplets over a microfabricated trench positioned diagonally in relation to the direction of flow, photo-tagged droplets were directed toward a different chip exit based on their lateral movement. The technique can be performed on a conventional fluorescence microscope and uncouples the observation and selection of droplets, thus enabling the selection on a large variety of signals, or based on qualitative user-defined features.
Identification and Characterization of “Candidatus Rickettsia Thierseensis”, a Novel Spotted Fever Group Rickettsia Species Detected in Austria
Authors: Schötta Anna-Margarita , Wijnveld Michiel , Höss Dieter , Stanek Gerold , Stockinger Hannes , Markowicz Mateusz
Published: October 2020

Rickettsia spp. are the second most common pathogens detected in Ixodes ricinus ticks in Austria after Borrelia burgdorferi sensu lato. Species belonging to the spotted fever group (SFG) are the causative agents for tick-borne rickettsiosis across the world. So far, only four SFG Rickettsia spp. were detected in Austria, namely R. helvetica, R. raoultii, R. monacensis and R. slovaca. Here, we describe the identification of a new SFG Rickettsia species detected in an I. ricinus tick. Sequencing of various rickettsial genes revealed a nucleotide sequence similarity of 99.6%, 98.5%, 97.3% and 98.5% to the gltA, ompA, ompB, and sca4 genes, respectively, of known and validated species. Additionally, sequencing of the htrA gene and 23S-5S intergenic spacer region also only showed 99.6% and 99.2%, respectively, similarity to known species. Therefore, and in accordance with current criteria for Rickettsia species discrimination, we hereby describe a new species of the SFG with putative pathogenic potential. We propose the name “Candidatus Rickettsia thierseensis” based on the village Thiersee in the Austrian province of Tyrol, where the carrying tick was found.
Production of Vanadium Concentrate from a Small-Scale Lead Vanadate Deposit by Gravity Concentration: A Pilot Plant Study
Authors: Silin Ivan , Gürsel Devrim , Kremer Dario , Hahn Klaus M. , Wotruba Hermann
Published: October 2020

The renewable energy revolution calls for high-performing materials and makes metallic compounds like lithium, cobalt, nickel and vanadium more and more critical. Innovations contribute to inventions and developments like vanadium redox flow batteries for large-scale energy storage systems with numerous technological advantages. Potential shortages of vanadium and its sources will contribute to turbulence in vanadium pricing. Nowadays, main sources and production sites of vanadium are located in Russia, China and South Africa. About 85% of vanadium applications are ferroalloys and high-performance alloys, which make production and price of vanadium dependent on the iron ore market. Partial covering of a potential vanadium demand may be achieved by an exploitation of lead vanadate ore deposits as alternative vanadium source. In the present work, the processing of a lead vanadate ore, mainly containing vanadinite and descloizite was investigated. Based on ore characterizations and preliminary beneficiation tests, a flowsheet was developed to design a small-scale processing plant, including comminution, dewatering and gravity separation. Preliminary laboratory tests and samples from the small-scale processing plant show promising results for the recovery of vanadium in a lead vanadate concentrate with a grade of 12 to 16% V2O5 and a recovery of 68 to 75%.