bluuebunny/crossref_metadata_2025 huggingface dataset:
Verifying the Effectiveness of Online Cause-Related Advertising: Fosused on Brand Relationship, Attachment, and Ethical Consumption Propensity
Authors: Zhao Xinsheng , Yoon Sungjoon , Park Yoonjoo
Published: May 2023

Cause-Related Marketing (CRM) has emerged as a new concept that links CSR activities to marketing, enabling corporations to pursue both building company-consumer partnership and gaining sustainable mutual benefits that are elicited from the partnership. Among various cause-related marketing activities, on-line cause-related advertising is the most commonly used one. The purpose of this study is to examine the effects of on-line cause-related advertising and moderating role of ethical consumption propensity. In particular, this study intends to contribute to successful development of on-line cause-related advertising strategy by focusing on the relationship between the four factors: customers' perception of on-line cause-related advertising, brand-consumer relationship, brand attachment and loyalty. The results of this study are as follows: First, customers' perception of on-line cause-related advertising was related positively to consumer-brand- relationship. Second, brand-consumer relationship was related positively to brand attachment and customer loyalty. Third, brand attachment was related positively to customer loyalty. Furthermore, from the consumer’s psychological viewpoint, ethical consumption propensity had a significant moderating effect to between customers' perception of cause-related advertising and brand-consumer relationship as well as brand attachment. The results of this study may contribute to the existing literature as an new empirical attempt to examine the effect of the on-line cause-related advertising.
Influence of Ion Nitriding on Microstructure and Properties of Haynes 282 Nickel Alloy Specimens Produced using Additive Technique
Authors: Sitek Ryszard , Kulikowski Krzysztof , Paradowski Krystian , Gancarczyk Kamil , Kobayashi Akira , Moneta J. , Kamiński Janusz
Published: May 2023

The paper investigates the influence of ion nitriding on the microstructure, corrosion resistance, and tensile strength at elevated temperatures of Haynes 282 nickel superalloy specimens produced by the Direct Metal Laser Sintering-DMLS technique. Analysis of the specimens’ surface morphology revealed that the ion nitriding process significantly affects the physical and chemical phenomena occurring on the surface of the specimens. Phase composition and stress analysis showed that Haynes 282 nickel alloy specimens produced by the DMLS technique and a chromium nitride layer produced in the ion nitriding process have tensile stress. The layer is also characterized by high nanohardness compared to specimens produced using the classical method (wrought) and DMLS technique. The conducted corrosion resistance tests confirmed the positive effect of the nitrided layer on the corrosion resistance of Haynes 282 nickel alloy produced by the DMLS technique. The ion nitriding process did not significantly affect the elevated-temperature tensile strength of Haynes 282 nickel alloy specimens produced by the DMLS technique.
A Bioinformatics Analysis of Ovarian Cancer Data Using Machine Learning
Authors: Schilling Vincent , Beyerlein Peter , Chien Jeremy
Published: May 2023

The identification of biomarkers is crucial for cancer diagnosis, understanding the underlying biological mechanisms, and developing targeted therapies. In this study we propose a machine learning approach to predict the outcome and platinum resistance status of ovarian cancer patients using public available gene expression data. Six classical machine learning algorithms are compared on their predictive performance. Those with the highest score are analyzed by their feature importance using the SHAP algorithm. We were able to select multiple genes that were correlating with the outcome and platinum resistance status of the patients and validated those using Kaplan-Meier plots. In comparison to similar approaches the performance of the models were higher and different genes using feature importance analysis were identified. The most promising identified genes that could be used as biomarkers are: TMEFF2, ACSM3, SLC4A1 and ALDH4A1.
Privacy-Aware Cloud Architecture for Collaborative Use of Patients’ Health Information
Authors: Alhaddadin Fadi , Gutierrez Jairo
Published: May 2023

Cloud computing appears to be the dreamed vision of the healthcare industry; it refers to means of storing and accessing data and programs over the internet instead of the computer’s hard drive. However, the adoption of cloud computing requires solving several issues and infor-mation privacy is a major one. This work proposes a cloud architecture design for the healthcare information system. The proposed architecture enables for storing and sharing information in a privacy-preserving manner. Patients’ information in the proposed architecture is divided into four categories identified in the case study data analysis. User identity management protocol (U-IDM) is employed for controlling the access to patients’ information, and patients have means of control over who can access their information. A scenario-based instantiation validated the proposed architecture's privacy-preserving patient data exchange. The instantiation proved that the proposed architecture allows sharing healthcare information without violating the privacy of patients.
Konektivitas Pada Area Rumah Susun Harapan Jaya di Kota Pontianak
Authors: Muyasarah Mutia , Sarwadi Ahmad
Published: May 2023

Pontianak City has six housing that have been built and inhabited. It is interesting to see the use of the housing by the occupants. There is a fact that the circulation route of Harapan Jaya Housing is not fully utilized according to plan. The purpose of this research is to reveal which of these circulation pathways are used by the occupants and identifying potential circulation pathways. The research focuses on circulation issues, which connectivity between areas has succeeded in supporting the occupants’s activities. The data collection method was carried out by field observations and interviews with the occupants to confirm the observations results. The analysis method uses depthmapX-space syntax software with an axial map to determine the level of connectivity and integration that occurs. This study also simulated the addition of alternative circulation pathways. The results indicate that the addition of circulation paths can improve connectivity within the housing area. Efficient and functional connectivity in housing can use two models of circulation path, namely linear or grid.
Kajian Keberlanjutan Sosial Ekonomi pada Destinasi Wisata Pos Lintas Batas Negara Skouw
Authors: Thane Grace Friscilia , Giriwati Novi Sunu Sri , Aziz Sri Utami , Kusdiwanggo Susilo
Published: May 2023

The Skouw PLBN needs to address and develop a number of things in order to draw more tourists, including local cuisine, cultural arts facilities, and sports facilities. To find out the urgency, an assessment was carried out with the parameters of the SDGs with three main pillars, namely the balance between aspects of the economic, social and environmental fields. Field observations and literature studies served as the data collection method. The global action plan's SDGs implementation is based on the 5 P principles (People, Planet, Prosperity, Peace, and Partnership). The 4 Ps must be examined for the SDGs' accomplishment in order to understand social and economic problems in society. The outcomes include a number of strategies on the topics of food accessibility and availability, healthy living, education, gender equality, as well as community involvement to collaborate with the government to develop the Skouw PLBN region in a social and economic sustainable manner.
Kajian Teori Konsep Healing Environment sebagai Strategi Desain pada Pusat Rehabilitasi Anak Terlantar
Authors: Muthiah Ukhtiya , Yuliani Sri , Triratma Bambang
Published: May 2023

The strategy for designing a rehabilitation center for abandoned children needs to pay attention to several criteria based on the characteristics of neglected children. The design criteria are arranged to get psychological comfort so that children can grow up with more productive activities, one of which can be through the application of the concept of a healing environment. The research aims to examine the theory of healing environment that fits the criteria of a rehabilitation center for neglected children as the basis for a design strategy. The research uses qualitative methods with comparative study techniques and theoretical reference comparisons. The result of the research is a design strategy based on the concept of healing environment which combines indoor and outdoor elements by exploiting the potential of the tropical climate related to natural lighting and ventilation.
Implementasi Arsitektur Semiotik pada Galeri Street Art di Kota Bandung
Authors: Subagyo Qonitah Hanan , Handayani Kusumaningdyah Nurul , Nirawati Maya Andria
Published: May 2023

Semiotic architecture is a bridge between architects and users who convey architectural information. The application of semiotic architecture to street art gallery buildings can be used as street art introduction images to users. Unfortunately, there is no research on the relationship between semiotic architecture and street art that can be used as design criteria parameters. Therefore, the urgency of this research is to find out which components of semiotic architecture are most suitable for the identity of street art galleries. The method of this research is studying the characteristics of street art and then analyzing it with three semiotic architectural theories from Yousafi, Uluğ and Huang & Zhou. This research produces the design criteria of a street art gallery, namely the use of polychromatic colors, the use of dominant walls, designing space without partitions, materials that give the impression of a street, and incorporating public street art interaction content.
The Current Situation and Development of Chinese Language Education in Indonesian Universities in the New Era: A Case Study of the Department of Chinese Language, Universitas Sebelas Maret
Authors: Phanata Stephanie
Published: May 2023

Looking at the development of Chinese language education in Indonesia from the perspective of teaching Chinese as a foreign language, over the past few decades, considerable achievements have been achieved in the promotion of the Chinese language in Indonesia, with an increasing number of people learning Chinese language, even evolving the trend of the global Chinese language. This article explores the current situation and prospects of Chinese language education in Indonesian universities and the challenges and problems of Chinese language education in Indonesian universities. In promoting the development of Chinese language education, the academic community has gradually brewed some new ideas, hoping to encourage the globalization of Chinese language education through a brand-new structure, so that there will be further room for further development.
A Study on Teaching Chinese Second Language Interjection Based on PACE Teaching Model
Authors: Lishen Yu , Qingqing Kong
Published: May 2023

The PACE teaching model combines the advantages of teaching a second language explicit grammar and implicit grammar. The PACE teaching model is introduced comprehensively, and the Chinese interjection"啊、唉、嗯、呦、哎呀、哦、嘛、呢" are selected and studied in the design of teaching Chinese second language interjection based on the PACE model. Finally, the applicability of the PACE teaching model to the teaching of Chinese second language interjection is analyzed from the perspectives of the advantages of the PACE teaching model, the difficulty of acquiring interjection and the characteristics of the textbook layout, which provides Chinese teachers with new ideas for teaching Chinese grammar.
IMPLEMENTASI HAK REHABILITASI DALAM PERTIMBANGAN HAKIM BAGI PELAKU TINDAK PIDANA PENYALAHGUNAAN NARKOTIKA BAGI DIRI SENDIRI (STUDI PUTUSAN NOMOR: 653/PID.SUS/2018/PT.MKS)
Authors: Meiranda Andi Luffi , Ginting Rehnalemken
Published: May 2023

Abstrak

Penelitian ini bertujuan untuk mengetahui dan menganalisis bagaimana implementasi hak rehabilitasi dalam pertimbangan hakim bagi pelaku penyalahgunaan narkotika bagi  sendiri dalam Putusan Nomor: 653/PID.SUS/2018/PT.MKS. Metode penelitian yang digunakan dalam penelitian kali ini adalah penelitian normatif dengan menggunakan tiga pendekatan, yaitu pendekatan studi kasus, pendekatan perundang-undangan, dan pendekatan konseptual, serta menggunakan teknik analisis secara kualitatif dengan metode silogisme yang bersifat deduksi. Jenis dan sumber bahan hukum yang digunakan adalah Putusan Nomor : 653/PID.SUS/2018/PT.MKS, Undang-Undang Nomor 35 Tahun 2009 tentang Narkotika, dan buku-buku hukum lain yang menunjang penelitian ini. Berdasarkan hasil penelitian ini implementasi pemberian hak rehabilitasi bagi penyalahguna narkotika bagi diri sendiri dalam pertimbangan hakim Nomor : 653/PID.SUS/2018/PT.MKS masih kurang dipertimbangkan, walaupun  peraturan-peraturan yang  ada sudah cukup memadai dalam proses pemenuhannya.
Kata kunci : tindak pidana, penyalahgunaan narkotika bagi diri sendiri, rehabilitasi

Abstract

This study aims to determine and analyze how the implementation of rehabilitation rights in the judges’ considerations for self-abuse of narcotics in the Verdict Number : 653/PID.SUS/2018/PT.MKS. The research method used in this research is normative research using three approaches, namely a case study approach, a statute approach, and conceptual approach, as well as using a qualitative analysis technique with a deductive syllogism method. Types and sources of legal materials used are Verdict Number : 653/PID.SUS/2018/PT.MKS, The Republic Of Indonesia Number 35 Year 2009 Concerning Narcotics, and other legal books that support this research. Based on the results of this study, the implementation of granting rehabilitation rights for narcotics abusers for themselves is still lacking in the Verdict Number : 653/PID.SUS/2018/PT.MKS, even though the existing regulations are sufficient in the fulfillment process.
Keywords : crime, self-abuse of narcotics, rehabilitation
JENIS DAN KORELASI KORBAN DENGAN PELAKU PADA KEJAHATAN PELECEHAN SEKSUAL DI INSTAGRAM
Authors: Wibowo Mustofa Ponco , Sulistyanta ,
Published: May 2023

Abstrak

Artikel ini mendeskripsikan dan mengkaji permasalahan, pertama jenis-jenis pelecehan seksual yang bagaimanakah yang dialami oleh korban, dan yang kedua bagaimana tinjauan viktimologi pelecehan seksual di media sosial instagram. Tujuan dari penelitian ini yaitu untuk menjelaskan jenis-jenis pelecehan seksual yang dialami korban dan tinjauan viktimologi pelecehan seksual di media sosial instagram. Dalam penelitian ini, peneliti menggunakan metode penelitian empiris dimana penelitian tersebut mengambil data dengan cara observasi lapangan, wawancara, dan studi kepustakaan. Penelitian ini dilakukan di media sosial instagram, kemudian peneliti mengambil sampel populasi sebanyak 20 (dua puluh) orang korban kejahatan pelecehan seksual di media sosial instagram dan menggolongkan jenis kejahatan pelecehan seksual sebanyak 5 (lima) jenis. Berdasarkan hasil penelitian menunjukkan bahwa sejumlah 11 orang mengalami semua jenis kejahatan pelecehan seksual, sejumlah 4 orang mengalami empat jenis pelecehan seksual, sejumlah 3 orang mengalami tiga jenis pelecehan seksual, dan sejumlah 2 orang hanya mengalami satu jenis pelecehan seksual saja. Kemudian meninjau kejahatan pelecehan seksual di media sosial instagram dalam perspektif viktimologi, peneliti mengacu pada 2 (dua) teori viktimologi dan 2 (dua) jenis viktimisasi.

Kata Kunci: Viktimologi, Pelecehan Seksual, Korban

Abstract

This article describes and examines the problem, firstly, what types of sexual harassment were experienced by the victim, and secondly, how to review the victimization of sexual harassment on social media Instagram. The purpose of this study is to explain the types of sexual harassment experienced by victims and to review the victimization of sexual harassment on social media Instagram. In this study, researchers used empirical research methods where the research took data by means of field observations, interviews, and literature studies. This research was conducted on Instagram social media, then researchers took a population sample of 20 (twenty) victims of sexual harassment crimes on Instagram social media and classified the types of sexual harassment crimes as 5 (five) types. Based on the results of the study, 11 people experienced all types of sexual harassment crimes, 4 people experienced four types of sexual harassment, 3 people experienced three types of sexual harassment, and a total of 2 people only experienced one type of sexual harassment. Then reviewing the crime of sexual harassment on social media Instagram from a victimization perspective, the researcher refers to 2 (two) theories of victimology and 2 (two) types of victimization.

Keyword: Victimology, Sexual Harassment, Victim
PENJATUHAN PIDANA DENDA TERHADAP ANAK YANG MELAKUKAN TINDAK PIDANA PERSETUBUHAN DALAM PUTUSAN NOMOR 1/PID.SUS-ANAK/2019/PN.PTS
Authors: Prastyoso ' , Subekti '
Published: May 2023

Abstrak
Penelitian hukum ini bertujuan untuk menganalisis penjatuhan pidana denda terhadap anak yang  melakukan tindak pidana persetubuhan dalam Putusan Pengadilan Negeri Putussibau Nomor 1/Pid. Sus-Anak/2019/PN.PTS. Penelitian ini menggunakan metode penelitian hukum normatif dengan sifat preskriptif. Pendekatan yang digunakan ialah pendekatan perundang-undangan dan pendekatan kasus. Bahan hukum yang digunakan terdiri dari bahan hukum primer dan bahan hukum sekunder. Teknik pengumpulan bahan hukum yang digunakan ialah studi kepustakaan. Teknik analisis yang digunakan ialah teknik deduksi dengan metode silogisme hukum. Hasil penelitian menunjukkan bahwa penerapan penjatuhan denda terhadap Anak telah diatur dalam Pasal 71 Ayat 3 Undang-Undang Nomor 11 Tahun 2012 yang menyatakan bahwa penjatuhan pidana denda harus digantikan dengan pelatihan kerja bagi Anak.
Kata Kunci : Pidana Denda, Anak, Tindak Pidana Persetubuhan.

Abstract
This legal research aims to analyze the imposition of fine for a child who commits sexuality within  The Verdict of Putussibau District Court Number 1/Pid.Sus-Anak/2019/PN.PTS. This research used normative legal research with prescriptive characteristic. This research used statute approach and case approach. The legal material consists of primary legal meterials and secondary legal materials, the legal sources was obtained by literature study. The legal sources was analyzed by using deductive sylogism law method. The result shows that Law Number 11 of 2012 concerning Juvenile Justice System has erased the provision on fine towards Juvenile Delinquency through the change of fine into work training form so they can’t be imposed by fine except in the form of work training. The Verdict of Putussibau District Court Number 1/Pid.Sus-Anak/2019/PN.PTS shows that is still such fine imposition towards Juvenile Delinquency which is not transferred into work training. The prosecutors and the judges in the mentioned verdict didn’t apply the provision of Article 71 paragraph 3 Law Number 11 of 2012 concerning Juvenile Justice System on indictment and conviction.
Keywords : Fines, Juvenile Delinquency, Sexuality
Morphological, physiological, and biochemical response of citrus rootstocks to salt stress
Authors: Arıkan Şeyma
Published: May 2023

Soil salinity is one of the most significant stressors limiting plant productivity in the world's irrigated and cultivated agricultural lands. Plants have a wide range of adaptations and mitigation strategies to overcome salinity effects. It is possible to mitigate the effects of salt stress by the development of resistant cultivars and more efficient use of available resources. However, these methods are time-consuming and costly. Solutions to salinity issues need to be developed that are both straightforward and inexpensive, as they will likely only be implemented temporarily. In this study, the effects of the different salinity levels (Control, 20mM, 40mM, 60mM, 80mM, and 100mM) on citrus rootstocks were determined. In the citrus growing regions, the sour orange, trifoliate orange, and Carrizo citrange were the three most popular rootstocks. As a result of the present study, the sour orange was found to have the lowest decline in plant height (13.36%), leaf area (31.19%), and root length (8.13%), while the trifoliate orange had the lowest decrease in fresh weight of the plant (6.82%) and root (4.42%). Trifoliate orange was shown to have the highest levels of catalase activity, whereas superoxide dismutase and peroxidase activities were found to be highest in Carrizo citrange and sour orange, respectively. The highest concentration of plant nutrients was found in the Carrizo citrange. Therefore, sour orange was discovered to be more salinity tolerant in terms of morphological traits, physiological qualities, biochemical composition, and plant nutrition than trifoliate orange and Carrizo citrange. For areas with high salt levels, growing citrus on sour orange rootstock may be a viable alternative
Determination of Adenosine phosphate in beef by QuEChERS- Graphene -UPLC
Authors: Duan Xiaoyan
Published: May 2023

A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef
Development of new two methods based on low-cost sensors for determination of calcium carbonate contents of soils
Authors: Karadöl Hayrettin
Published: May 2023

The chemical analysis of CaCO3 contents of soils provide information about not only efficiency of soil for supplying plant with nutrients but also identification of factors affecting this efficiency in the soil. The aim of the current experiment was to develop new methods based on sensors and compare with conventional Scheibler method. The CO2 liberated by the action of HCl on CaCO3 content of the soil in a reaction bottle and an accumulation chamber was determined by pressure sensor and CO2 sensor, respectively. The CaCO3 contents of soils were estimated using the regression equation of standard curves. The CaCO3 contents of soils obtained using Scheibler calcimetric, pressure and CO2 sensor methods ranged from 0.20 to 54.74%, 0.25 to 54.10% and 0.55 to 53.05%, respectively. On the basis of linearity of calibration curve and high correlation with Scheibler calcimetric method it can be said that developed pressure and CO2 sensor methods appears to be very useful for quantification of CaCO3 contents of soils. The pressure sensor has provided the opportunity in developing a simple and handy device with an affordable cost to measure CaCO3 contents of soils as accurately as Scheibler calcimeter when compared with CO2 sensor method. Even if there is a good relationship between Scheibler calcimetric method and CO2 sensor method the cost of CO2 sensor method may limit the use in determination of CaCO3 contents of soils. However, CO2 sensor method can be used to monitor CO2 evolved in biochemical process such as respiration and fermentation
The sweat fatty acid content of Holstein and Jersey cows in Summer
Authors: Anitaş Özgül
Published: May 2023

Sweat is important in thermoregulation, helping to dissipate heat in the body by evaporation in hot conditions in dairy cows. The purpose of this study was to evaluate the variation of sweat fatty acid compositions in summer conditions in Jersey and Holstein dairy cattle for their potential thermoregulatory roles in animal health protection. Temperature, relative humidity and Temperature-Humidity Index (THI) values were calculated for the intensity of stress in the indoor environment. The chemical compounds of sweat were determined by gas chromatography device and inter-breeds differences were revealed. As a result of analyzes, a total of 23 fatty acids were detected in both breeds. It was understood that there were 15 fatty acids in the Jersey breed. Palmitic acid was detected at a rate of 27.72 % in Holstein cows and 25.16 % in jersey cows that may be due to a higher rate of fatty acid release from muscle tissue, so that it may be less resistant than the jersey breed in heat stress conditions. It can be said that palmitic acid can be an indicator for heat stress conditions. ∑SFA and ∑PUFA contents of the sweat of Holstein cows were lower than those of Jersey breeds while ∑MUFA content was higher than those of Jersey breeds. The high expulsion of ∑SFA and ∑PUFA in the sweat of the Jersey cows can provide the function of delaying the body's dehydration by forming a hydrolipid film on the skin surface in hot conditions
Characterization and evaluation of the diversity of local and imported coconut varieties collected from the Sultanate of Oman
Authors: Ghribi Sami
Published: May 2023

Coconut (Cocos nucifera L.) is commonly recognized by their heights, prevalence and long-term presence. Currently the demand for coconuts and these products is high all over the world, it is in this context that our research work is included in order to preserve and use the rich biological diversity of this palm. The main objective of this study was to determine the effect of the environment on the morphology of different organs of the coconut palm. Principal component analysis (PCA) is one of the most important statistical tools for estimating the variations between the morphological characters of individuals, which are the basis for determining the degree of polymorphism, for detecting relationships between cultivars, and for evaluating the agronomic traits for the classification of coconuts. This study was conducted on twelve coconut genotypes spread over in the Sultanate of Oman and different thirty-nine qualitative and quantitative traits were evaluated. This data set was analyzed by the principal component analysis (PCA) and UPGMA cluster. And these; the results revealed great variability in the expression of the vegetative (quantitative and qualitative) agromorphological characters related to the stipe, the leaf and the fruit. The results showed that local cultivar individuals were morphologically distinct, suggesting that it may be a polyclonal variety
Biological control of root rots on alfalfa with Streptomyces
Authors: Liu Lianna
Published: May 2023

In the current study, strain D1 was screened from saline-alkali soil of grassland by the method of plate confrontation. The inhibition rates against Fusarium equiseti BS1 , Fusarium oxysporum BF28 , Fusarium oxysporum BF19 and Fusarium solami BF36-2 were 70.81% , 76.00% , 67.80% and 65.70% respectively. Strain D1 was identified as Streptomyces ciscaucasicus by observation of colony morphology, physiological and biochemical characteristics and sequence analysis of 16S rRNA gene. The results of antimicrobial spectrum showed that the strain had antagonistic effect against 9 kinds of plant pathogenic fungi. The control effect of the strain on alfalfa root rot was 52.8% and it could promote rooting. The results showed that the strain D1 had potential as a biological agent
The role and mechanism of calcitriol in promoting intestinal injury in AKI by improving the intestinal barrier
Authors: Huo Aijing , FengmeiWang  , Xia Jingjing , Wang Haoyu
Published: May 2023

Background：The intestinal microbiota affects the inflammatory status of the host and the prognosis of diseases. AKI can lead to microinflammation of intestinal tract and changes of intestinal flora. Calcitriol (Cal) has a protective effect on the intestine.
Methods and Results：In the AKI rat model, intestinal infiltration of inflammatory cells and the deterioration of renal function were significantly alleviated by Cal pretreatment. By increasing the levels of Zonula Occludens-1(ZO-1 and Occludin), Cal significantly prevented the destruction of the intestinal barrier in AKI. In AKI rats, Cal decreased the concentration of Proteobacteria and enhanced the linear discriminant (LDA) score of beneficial bacteria (such as Lactobacillus). Cal increased the expression of butyric acid among intestinal metabolites. Cal supplementation decreased serum LPS levels and downstream HMGB1-MYD88-NF-κB-p65 signaling.
Conclusions：High-dose Cal may play a protective role in AKI by regulating HMGB1-MYD88-NF-κB-p65 signaling pathways and the intestinal microbiota in rats. This study demonstrates the renal effects of Cal. The experiment revealed a close interaction between the kidney and the intestine. It provides new insights for the treatment of AKI.
Association Between the Oxidative Stress Gene Polymorphism and Chronic Obstructive Pulmonary Disease Risk: A Meta-Analysis
Authors: Zhou Ting , Zuo Qiunan , Chen Mengchun , Zhao Yingying , Li Xiaohui , Guo Shujin
Published: May 2023

Background The association between the oxidative stress gene polymorphism and chronic obstructive pulmonary disease (COPD) risk has been extensively studied but the results have been controversial. This study aimed to investigate the overall association between the oxidative stress gene polymorphism and the risk of COPD.Methods We searched the PubMed and EMBASE database to identify studies that investigated the association between the oxidative stress gene polymorphism and risk of COPD. The relevant data were extracted and statistical analyses were performed using the Revman 5.4 and STATA 12 software. Dominant genetic model, recessive model, co-dominant model, heterozygote model, and allele model were analyzed. Venice criteria and publication bias were conducted to access the credibility and reliability.Results A total of 63 publications were included in the meta-analysis.15 genetic variants of 6 genes were analyzed, and seven SNP in GSTP1, CAT, CYP, SOD were first analyses until now. In our study, EPHX T113C C allele, GSTM1 null, GSTT1 null, GSTP1 A313G G and C341T T allele, CYP1A1 MspI C allele, SOD3 A213G G allele and L type in Ho-1 showed increased COPD risk, especially in Asians. T allele in CAT C262T and C allele in SOD2 Val 9 Ala were associated with decreased COPD risk. Subgroups analysis were performed in accord with HWE and ethnicity. Publication bias was assessed by Begg’s funnel plots and Egger’s test, and no publication bias were found for recessive models. 4 variants were identified with strong levels of epidemiological evidence of associations with the COPD risk.Conclusions Our results confirm that oxidative stress gene polymorphism was associated with COPD risk. Well-designed studies with large sample sizes are essential to clarify the association of these significant variants with the susceptibility to COPD.
How are protective behaviors against COVID-19 socially controlled?
Authors: Mejia Cristian Yesid Urbano , Gallardo Juan Pablo Molano , Villalba Julián Zanguña , Clavijo Arturo
Published: May 2023

During the COVID-19 pandemic, individuals adjusted their adherence to protective behaviors according to the social situation. Also, social consequences partially influenced, given that they were the most contiguous and likely to adherence behavior. The behavior analysis framework can help to understand the social contingencies that maintain adherence on a large scale in natural settings. This study assessed how social antecedents and consequences influenced adherence to protective behaviors. The antecedents were indoor and outdoor. The social consequences were receiving social approval, avoiding social disapproval, receiving a social rejection, and losing gratification in social relationships. The protective behaviors were mask-wearing, physical distancing, hand-washing, and correcting others. Colombian residents completed an online survey based on the indirect functional assessment in which they reported their adherence to the social contexts and the perceived social contingencies. People reported higher adherence to protective behaviors in the outdoor context than indoors. Social approval was the main reinforcer, and losing gratification in social relationships was the main punisher. Also, avoiding being judged worked as a reinforcer, and receiving social rejection worked as a punisher. Social context interacted with social consequences. The effect of consequences under adherence behavior was more intense in indoor than outdoor contexts. Mask-wearing and physical distancing were the more socially controlled protective behaviors. We demonstrated that the probability of certain social consequences partially explained adherence's subjective value. Our results about social approval were consistent with injunctive norm literature. Social distance with people involved in each social context can explain our findings.
Design and Analysis of Nonlinear Numerical Algorithm for Seismic Response of Structures Based on HVSR Algorithm
Authors: Wang Shuai , Wang Chao , Zhang Zongbao
Published: May 2023

Earthquakes are one of the main factors that cause structural disasters in current buildings. Under the action of earthquakes, adjacent building structures are generally in different vibration phases, and the structures are likely to collide with each other, and collisions may cause serious damage to the structure. In order to prevent damage to the designed buildings under certain earthquakes, this paper mainly focuses on the design and analysis of numerical algorithms for seismic nonlinear structural dynamic responses based on the HVSR algorithm. In the two-dimensional finite element analysis in this paper, the dam rockfill body adopts four-node isoparametric elements, and the equivalent linear model related to dynamic shear strain is used to reflect the dynamic nonlinear characteristics of the dam material. When the dynamic response is used as the constraint condition, the dynamic response can be used to judge whether the design variables meet the requirements; when the dynamic response is used as the optimization target, it is the optimization result of each step. Considering the importance of the instantaneous amplitude of acceleration response in the correction of nonlinear structure model. The acceleration response time series is divided into 15 time periods uniformly, and then the position corresponding to the peak point of the instantaneous amplitude in each time period is selected as the selected data point position. Apply the same seismic load to the bottom of the established nonlinear model, extract the dynamic response data of the top layer of the structure, and then extract the instantaneous amplitude of the main component of the structural dynamic response through the time-varying filter and Hilbert transform based on discrete analytical mode decomposition and the corresponding the instantaneous frequency. Under the action of these four ground motions, the collision force in the range of 0~50kN accounts for more than 87% of the total number of collisions. The results show that the HVSR algorithm can obtain the instantaneous characteristic parameters of the dynamic response of the nonlinear structure and realize the correction of the model.
2-year survival and cost analysis of occlusoproximal ART restorations using encapsulated glass ionomer cement in primary molars: a randomized clinical trial
Authors: Garbim Jonathan Rafael , Saihara Cintia Saori , Olegário Isabel Cristina , Hesse Daniela , de Araujo Mariana Pinheiro , Bonifácio Clarissa Calil , Braga Mariana Minatel , Raggio Daniela Prócida
Published: May 2023

Background
Encapsulated glass ionomer cement is a promising option for atraumatic restorative treatment due to its potential to minimize the operator factor. This study aimed to compare two encapsulated glass ionomer cements, Equia Forte (GC Corp) and Riva Self Cure (SDI), in terms of their survival rates and costs for restoring occluso-proximal cavities in primary molars. The study was conducted in the city of Tietê, Brazil, and followed up with children over a period of 24 months.
Methods
Children aged 4–8 years with occlusal-proximal dentin carious lesions in primary molars were randomly assigned to receive either Equia Forte (EF) or Riva Self Cure (RSC). The primary outcome was 24-month restoration survival, analyzed using Kaplan-Meier survival analysis, Cox regression, and intention-to-treat (ITT) analysis. Secondary outcomes included a 24-month restoration cost analysis using Monte-Carlo simulation. Treatment was performed by two trained final-year students in a school setting, and one trained and calibrated examiner evaluated the restorations after 2, 6, 12, 18, and 24 months.
Results
A total of 152 children (76 in EF group and 76 in RSC group) were included in the study, and 135 (88.8%) were followed up at 24 months. There was no significant difference in the primary outcome between the two groups, with an overall restoration survival rate of 39% (45% in EF and 32% in RSC). However, there was a significant difference in the estimated incremental cost between the two groups, with RSC being more cost-effective from the Brazilian perspective (EF cost: $25.48, RSC cost: $19.30, incremental cost: US$6.18).
Conclusion
After two years of follow-up, Riva Self Cure showed comparable restoration survival to Equia Forte, while being more cost-effective from the Brazilian perspective. Encapsulated glass ionomer cements are a viable option for atraumatic restorative treatment, and their cost-effectiveness should be considered when selecting a material for such treatments.
Trial Registration
This randomized clinical trial was registered on ClinicalTrials.Gov - NCT02730000 (06/04/2016)
The role of genetic factors in different outcomes of pediatric myelodysplastic syndromes
Authors: Li Ying , Cheng Li , Peng Yun , Wang Lin , Zhang Wenzhi , Yin Yuhong , Zhang Jing , Wu Xiaoyan
Published: May 2023

Background
To explore the role of genetic factors in the pathogenesis of myelodysplastic syndrome (MDS) in children with different outcomes and to discover the correlation between genetic features and clinical outcomes as well as disease characteristics.
Methods
We analyzed the archived genetic data from 26 patients who were diagnosed of pediatric MDS at our institution between 2015 and 2021, and evaluated the association between different genetic characteristics and clinical manifestations as well as prognosis. We also took 3 patients with different genetic background and outcomes as examples to elaborate the role of genetic factors in different prognoses.
Results
Genetic variations were found in 13 (8 RCC, 4 MDS-EB, 1 MDS-EB-t, the latter two types are classified as advanced MDS) of the 26 patients. In RCC group, transfusion dependency (2/4 vs. 1/4) and disease progression (2/4 vs. 1/4) occurred more frequently in patients with co-occurrence of somatic and germline mutations (CSGMs) than those with somatic mutations alone. CSGMs (4/5 in advanced MDS, 4/8 in RCC), germline pathogenic variants (2/4 in advanced MDS, 1/4 in RCC) and somatic mutations specifically associated with MDS (6/6 in advanced MDS, 8/15 in RCC) were also identified in our study. Three patients with distinct genetic variations taken as examples in our study presented evidently different clinical outcomes. Case 1 with germline and somatic mutations of unknown significance had a relatively slow disease course and a good prognosis. Case 2 with compound heterozygous germline SBDS variants and somatic mutations such as del20q had a reversed disease outcome and a stable clinical course. Case 3 with a germline GATA2 variant and somatic mutations including − 7 had a rapidly progressive course of disease and a worst prognosis.
Conclusion
Our findings indicate that genetic background of pediatric MDS is closely linked with disease characteristic as well as outcome and that CSGMs leads to disease progression and poor clinical manifestations. It should be emphasized that the interaction between certain germline variants and somatic mutations, such as SBDS and del20q, may result in hematopoietic stem cell adaptation (improve hematopoiesis) and reverse adverse clinical outcomes, which can facilitate the development of targeted therapy.
Screening of the antileishmanial and antiplasmodial potential of synthetic 2-arylquinoline analogs
Authors: Espinosa-Saez Roger , Robledo Sara M. , Pineda Tatiana , Murillo Javier , Zúñiga César , Yañez Osvaldo , Cantero-López Plinio , Saez-Vega Alex , Guzmán-Teran Camilo
Published: May 2023

In this study, six analogs of 2-arylquinoline were synthesized and evaluated for their in vitro and in vivo antiplasmodial and leishamanicidal activity. Also, hemolytic activity and drugability were tested in vitro and in silico, respectively. In relationship to leishmanicidal activity, the compounds showed half maximal effective concentration (EC50) values between 3.6µM and 19.3µM. Likewise, treatment using compounds 4a-f caused improvement in most of treated hamsters and cured some of them. Regarding antiplasmodial activity, the compounds showed moderate to high activity, did not show hemolytic activity. Furthermore, 4e and 4f compounds were not able to control P. berghei infection when administered to animal models. Molecular dynamic simulations, molecular docking and ligand binding affinity indicate good bioavailability and absorption characteristics of the studied compounds, which are expected to be active when administered orally. The compounds are absorbable at the hematoencephalic barrier but not in the gastrointestinal tract. ADMET properties suggest that these molecules may be used as a safe treatment for Leishmania.
Large-scale networks changes in Wilson's disease associated with neuropsychiatric impairments: a resting-state functional magnetic resonance imaging study
Authors: Wang Anqin , Dong Ting , Wei Taohua , Wu Hongli , Yang Yulong , Ding Yufeng , Li Chuanfu , Yang Wenming
Published: May 2023

Background In Wilson’s disease (WD) patients, network connections across the brain are disrupted, affecting multidomain function. While the details of this neuropathophysiological mechanism remain unclear due to the rarity of WD. In this study, we aimed to investigate alterations in brain network connectivity at the whole-brain level (both intra- and inter-network) in WD patients through independent component analysis (ICA) and the relationship between alterations in these brain network functional connections (FCs) and clinical neuropsychiatric features to understand the underlying pathophysiological and central compensatory mechanisms.Methods 85 patients with WD and age- and sex-matched 85 healthy control (HC) were recruited for resting-state functional magnetic resonance imaging (rs-fMRI) scanning. We extracted the resting-state networks (RSNs) using the ICA method, analyzed the changes of FC in these networks and the correlation between alterations in FCs and clinical neuropsychiatric features.Results Compared with HC, WD showed widespread lower connectivity within RSNs, involving default mode network (DMN), frontoparietal network (FPN), somatomotor network (SMN), dorsal attention network (DAN). Furthermore, the decreased FCs in the left medial prefrontal cortex (L_ MPFC), left anterior cingulate gyrus (L_ACC), precuneus (PCUN)within DMN were negatively correlated with the Unified Wilson’s Disease Rating Scale-neurological characteristic examination (UWDRS-N), and the decreased FCs in the L_MPFC, PCUN within DMN were negatively correlated with the Unified Wilson’s Disease Rating Scale-psychiatric symptoms examination (UWDRS-P). We additionally discovered that the patients with WD exhibited significantly stronger FC between the FPN and DMN, between the DAN and DMN, and between the FPN and DAN compared to HC.Conclusions we have provided evidence that WD is a disease with widespread dysfunctional connectivity in resting networks in brain, leading to neurological features and psychiatric symptoms (e.g. higher-order cognitive control and motor control impairments). The altered intra- and inter-network in the brain may be the neural underpinnings for the neuropathological symptoms and the process of injury compensation in WD patients.
MicroRNA146a-loaded Magnesium Silicate Nanospheres promote bone regeneration with inflammatory microenvironment
Authors: Chen Qianming , Yang Jiakang , Jing Shuai , Siow Lixuen , Lu Jingyi , Sun Miao , An Wenyue , Yu Mengfei , Wang Baixiang
Published: May 2023

The reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a clinical challenge as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSN) to load a nucleic acid drug - microRNA146a-5p (miR146a), fabricating a nanobiomaterial complex MSN-miR146a with high oligo transfection efficiency. This complex showed a potent promoting effect on osteogenic differentiation of human dental pulp stem cells with upregulated expression of osteogenesis markers including alkaline phosphatase, collagen Ⅰ, Runt-related transcription factor 2, osteopontin and Osterix. Additionally, miR146a was observed to have an anti-inflammatory effect by reducing CD40high M1 pro-inflammatory mouse bone marrow derived macrophages under lipopolysaccharide stimulation, with MSN simultaneously upregulating Arginase-1high or CD163high M2 pro-regenerative BMMs. The osteogenic-promoting and immunoregulatory effects of MSN-miR146a were further validated in a mouse-infected mandibular bone defect model delivered by photocuring hydrogel. Collectively, the MSN-miR146a complex revealed great potential in treating inflammatory irregular oral-maxillofacial bone defects.
Nanoparticles with adaptable self-assembled shells sense and inhibit influenza virus at ultra-low concentrations
Authors: Sergeeva Yulia , Yeung Sing Yee , Hix-Janssens Thomas , Sellergren Börje
Published: May 2023

Multivalent inhibitors that mimic the polysaccharide array on cells represent a new paradigm in the development of antiviral agents and antibiotics. Covalent ligand anchoring limits the affinity and in turn potency of these inhibitors with dissociation constants (Kd) commonly found in the micromolar or upper nanomolar range. Addressing this deficiency we here report on easily accessible gold core-shell nanoparticles (rSAM-NPs) featuring adaptable reversible self-assembled monolayer (rSAM) -based shells. The rSAMs are anchored by non-covalent amidinium-carboxylate interaction on gold nanoparticles at slightly alkaline pH resulting in laterally mobile pH-responsive assemblies that are functional at physiological pH. Introducing sialic acid ligands in the shell, we show that the rSAM-NPs strongly interact with the influenza virus surface protein hemagglutinin (limit of detection LoD < 2 nM) and deactivated bird flue virus (LoD < 1 HAU) in allantoic liquid. Finally, we show that the rSAM-NPs effectively inhibit the interaction of the virus with red blood cells at concentrations in the low picomolar range. This represents a more than 1000-fold increased potency with respect to monosaccharide-based multivalent inhibitors.