bluuebunny/crossref_metadata_2025 huggingface dataset:
SiC-MOSFET or Si-IGBT: Comparison of Design and Key Characteristics of a 690 V Grid-Tied Industrial Two-Level Voltage Source Converter
Authors: Fuentes Carlos D. , Müller Marcus , Bernet Steffen , Kouro Samir
Published: May 2021

In this paper, a design driven comparison between two 190 kVA industrial three-phase two-level voltage source converter (2L-VSC) designs based in silicon carbide (SiC) and silicon (Si) for 690 V grids is presented. These two designs were conceived to have the same nominal power, while switching at reasonable switching speeds and requiring the same case to ambient thermal impedance. Under these conditions, the designs were studied to detect the potential gains and limitations that a pragmatic converter design could feature when using these two technologies regarding cost, efficiency, size and weight. To achieve this, experimentally determined semiconductor characteristics were used to perform simulations, the results of which were then used to design the essential parts of the converter. These designed parts were then corroborated with manufacturers, from which physical characteristics of all designed components were obtained. The results show that the SiC based design presents substantial weight savings and an 11% system cost reduction, while preserving its traditional characteristics such as improved overall efficiency when compared to the silicon based design under the given design requirements and constraints.
Swarm Intelligence-Based Methodology for Scanning Electron Microscope Image Segmentation of Solid Oxide Fuel Cell Anode
Authors: Chalusiak Maciej , Nawrot Weronika , Buchaniec Szymon , Brus Grzegorz
Published: May 2021

Segmentation of images from scanning electron microscope, especially multiphase, poses a drawback in their microstructure quantification process. The labeling process must be automatized due to the time consumption and irreproducibility of the manual labeling procedure. Here we show a swarm intelligence-driven filtration methodology performed on raw solid oxide fuel cell anode’s material images to improve the segmentation methods’ performance. The methodology focused on two significant parts of the segmentation process, which are filtering and labeling. During the first one, the images underwent filtering by applying a series of filters, whose operation parameters were determined using Particle Swarm Optimization upon a dedicated cost function. Next, Seeded Region Growing, k-Means Clustering, Multithresholding, and Simple Linear Iterative Clustering Superpixel algorithms were utilized to label the filtered images’ regions into consecutive phases in the microstructure. The improvement was presented for three different metrics: the Misclassification Ratio, Structural Similarity Index Measure, and Mean Squared Error. The obtained distribution of metrics’ performances was based on 200 images, with and without filtering. Results indicate an improvement up to 29%, depending on the metric and method used. The presented work contributes to the ongoing efforts to automatize segmentation processes fully for an increasing number of tomographic measurements, particularly in solid oxide fuel cell research.
The Influence of Rate of Change in Confining and Pore Pressure on Values of the Modulus of Compressibility of the Rock Skeleton and Biot’s Coefficient
Authors: Nowakowski Andrzej
Published: May 2021

This work discusses the results of a study of the influence of rates of change of confining pressure on the result of a drained compressibility tests intended to determine the modulus of compressibility of a rock skeleton Ks. A series of cyclical compressibility tests was performed on samples of sandstone soaked in kerosene, for various rates of compression and decompression of the pressure liquid filling the cell and the pore volume of the sample. The studies showed that the deformability of the tested sample was directly proportional to the rate of change of the confining pressure. As a consequence, the value of the Ks modulus and Biot coefficient α decreased with increasing sample load rate. This phenomenon should be attributed primarily to equilibration of the liquid pressure inside the high-pressure cell with the liquid pressure in the sample pore space, caused by filtration of the pore liquid. These phenomena prove that the filtration process impacts the values of the modulus of compressibility of the rock skeleton Ks and of Biot coefficient α determined on the basis of the experiment. This is significant in the context of the use of Biot equations as constitutive equations for a porous rock medium.
Review of Vibration Control Methods for Wind Turbines
Authors: Awada Ali , Younes Rafic , Ilinca Adrian
Published: May 2021

The installation of wind energy increased in the last twenty years, as its cost decreased, and it contributes to reducing GHG emissions. A race toward gigantism characterizes wind turbine development, primarily driven by offshore projects. The larger wind turbines are facing higher loads, and the imperatives of mass reduction make them more flexible. Size increase of wind turbines results in higher structural vibrations that reduce the lifetime of the components (blades, main shaft, bearings, generator, gearbox, etc.) and might lead to failure or destruction. This paper aims to present in detail the problems associated with wind turbine vibration and a thorough literature review of the different mitigation solutions. We explore the advantages, drawbacks, and challenges of the existing vibration control systems for wind turbines. These systems belong to six main categories, according to the physical principles used and how they operate to mitigate the vibrations. This paper offers a multi-criteria analysis of a vast number of systems in different phases of development, going from full-scale testing to prototype stage, experiments, research, and ideas.
A Multiple Criteria Decision Making Method to Weight the Sustainability Criteria of Equipment Selection for Surface Mining
Authors: Patyk Michał , Bodziony Przemysław , Krysa Zbigniew
Published: May 2021

Selection and assessment of mining equipment used in open pit rock mines relies chiefly on estimates of overall exploitation cost. The rational arrangement of mining equipment and systems comprising loading machines, haul trucks and crushing plants should be preceded by a thorough analysis of technical and economic aspects, such as investment outlays and the costs of further exploitation, which largely determine the costs of mining operations and the deposit value. Additionally, the operational parameters of the mining equipment ought to be considered. In this study, a universal set of evaluation criteria has been developed, and an evaluation method has been applied for the selection of surface mining equipment and the processing system to be operated in specific mining conditions, defined by the user. The objective of this study is to develop and apply the new methodology of multi-criteria selection of open pit rock mining equipment based on multiple criteria decision-making (MCDM) procedures, to enable the optimization of loading, handling and crushing processes. The methodology, underpinned by the principles of MCDM, provides the dedicated ranking procedures, including the ELECTRE III. The applied methodology allows the alternative options (variants) to be ranked accordingly. Ultimately, a more universal methodology is developed, applicable in other surface mines where geological and mining conditions are similar. It may prove particularly useful in selection and performance assessment of mining equipment and process line configurations in mining of low-quality rock deposits. Therefore, we undertook to develop universal criteria and applications for the selection and performance assessment of process machines for surface mines, taking into account environmental aspects as well as deposit quality.
Cybersecurity Considerations for Grid-Connected Batteries with Hardware Demonstrations
Authors: Culler Megan , Burroughs Hannah
Published: May 2021

The share of renewable and distributed energy resources (DERs), like wind turbines, solar photovoltaics and grid-connected batteries, interconnected to the electric grid is rapidly increasing due to reduced costs, rising efficiency, and regulatory requirements aimed at incentivizing a lower-carbon electricity system. These distributed energy resources differ from traditional generation in many ways including the use of many smaller devices connected primarily (but not exclusively) to the distribution network, rather than few larger devices connected to the transmission network. DERs being installed today often include modern communication hardware like cellular modems and WiFi connectivity and, in addition, the inverters used to connect these resources to the grid are gaining increasingly complex capabilities, like providing voltage and frequency support or supporting microgrids. To perform these new functions safely, communications to the device and more complex controls are required. The distributed nature of DER devices combined with their network connectivity and complex controls interfaces present a larger potential attack surface for adversaries looking to create instability in power systems. To address this area of concern, the steps of a cyberattack on DERs have been studied, including the security of industrial protocols, the misuse of the DER interface, and the physical impacts. These different steps have not previously been tied together in practice and not specifically studied for grid-connected storage devices. In this work, we focus on grid-connected batteries. We explore the potential impacts of a cyberattack on a battery to power system stability, to the battery hardware, and on economics for various stakeholders. We then use real hardware to demonstrate end-to-end attack paths exist when security features are disabled or misconfigured. Our experimental focus is on control interface security and protocol security, with the initial assumption that an adversary has gained access to the network to which the device is connected. We provide real examples of the effectiveness of certain defenses. This work can be used to help utilities and other grid-connected battery owners and operators evaluate the severity of different threats and the effectiveness of defense strategies so they can effectively deploy and protect grid-connected storage devices.
Effects of the Chromium Content in (TiVNb)100−xCrx Body-Centered Cubic High Entropy Alloys Designed for Hydrogen Storage Applications
Authors: Strozi Renato Belli , Leiva Daniel Rodrigo , Zepon Guilherme , Botta Walter José , Huot Jacques
Published: May 2021

In this paper, we report an investigation of adding a non-hydride forming element in the multicomponent Ti-V-Nb-M system. By the Calculation of Phase Diagrams approach (CALPHAD), the thermodynamic phase stability of the TiVNbT (T = Cr, Mn, Fe, Co, and Ni) was investigated, and Cr was selected as the fourth alloying element due its high tendency to stabilize body-centered cubic solid solutions (BCC). The (TiVNb)100−xCrx alloys (with x = 15, 25, and 35 at.% Cr) were synthesized by arc-melting. The structural characterization reveals that the three alloys were composed of a major BCC phase, which agrees with the thermodynamic calculations. The three alloys absorb hydrogen at room temperature without any activation treatment, achieving a hydrogen uptake of about H/M = 2. The Pressure-Composition-Isotherms curves (PCI) has shown that increasing the Cr amount increases the equilibrium pressures, indicating that tunable H storage properties can be achieved by controlling the alloys’ Cr content.
Research on Online Diagnosis Method of Fuel Cell Centrifugal Air Compressor Surge Fault
Authors: Zhou Su , Jin Jie , Wei Yuehua
Published: May 2021

Stable operation of fuel cell air compressions is constrained by rotating surge in low flowrate conditions. In this paper, a diagnosis criterion based on wavelet transform to solve the surge fault is proposed. First of all, the Fourier transform was used to analyze the spectral characteristics of the outlet flowrate. Before wavelet transform was used, the data are standardized. This step eliminated the influence of the flowrate’s absolute value. Then, the wavelet coefficients under characteristic frequencies were extracted. Finally, the diagnosis criterion’s threshold, which indicates the surge occurrence, was defined from the perspective of safety margin. The criterion threshold alerted a surge only 1 s after it occurred. The analysis results show that the criterion meets with the expectation, and it can be used for the control of anti-surge valve.
Effect of Pole and Slot Combination on the AC Joule Loss of Outer-Rotor Permanent Magnet Synchronous Motors Using a High Fill Factor Machined Coil
Authors: Park Soo-Hwan , Lee Eui-Chun , Lee Gi-Ju , Kwon Soon-O. , Lim Myung-Seop
Published: May 2021

This paper proposes a design guideline for selecting the pole and slot combination of an outer-rotor permanent magnet synchronous motor (PMSM) using a maximum slot occupation (MSO) coil. Because the MSO coil has a large conductor area, the AC Joule loss in the conductors may be increased at high frequencies. To ensure high-efficiency for the PMSM, it is necessary to reduce the loss. Thus, it is important to select the pole- and slot- combination that has the minimum AC Joule loss. The loss is caused by skin/proximity effects and variations in the slot leakage flux. The skin effect is due to the armature winding and the variation in the slot leakage flux is due to the field flux. A method for separating the AC Joule loss due to each component using the frozen permeability method is proposed. Based on the proposed method, the effect of each cause on the loss at various pole- and slot- combinations is analyzed in this study.
Scaling up Renewable Energy Assets: Issuing Green Bond via Structured Public-Private Collaboration for Managing Risk in an Emerging Economy
Authors: Fu Jingyan , Ng Artie W.
Published: May 2021

Green bonds have increasingly been utilized around the world as a source of financing for renewable energy development, designed with compliance requirements and measurable economic returns to investors, while mitigating climate change. However, the efficacy of green bond arranged in the emerging economies for financing renewable energy assets and how the underlying risks are managed have remained to be explored. The paper aims to examine the evolving green financial system sponsored by both public and private institutions in managing such risks within China’s emerging economy. A case study of green financing for a bundle of wind power assets led by a state-owned enterprise (SOE) reveals an alternative approach by structuring public–private collaboration while stipulating market-based financial incentives to institutional stakeholders under a political economy. This institutional consortium is composed of a state development bank, a commercial bank, credit rating agencies, institutional and private investors, regional power purchasers, and carbon trading entities. Financial stakeholders’ risk in such emerging sustainable investment is moderated by these participating institutions and structured “upsides” from carbon trading aligned with the framework of green finance and standards for green bond development. The results reveal the potentials of scaling up the development of renewable energy by adequately managing and sharing key risks, while allocating substantial funding into renewable energy projects under such a green financial system that is to be complementary with a scalable post COVID-19 economic recovery.
Analysis of Haloferax mediterranei Lrp Transcriptional Regulator
Authors: Matarredona Laura , Camacho Mónica , García-Bonete María-José , Esquerra Belén , Zafrilla Basilio , Esclapez Julia , Bonete María-José
Published: May 2021

Haloferax mediterranei is an extremely halophilic archaeon, able to live in hypersaline environments with versatile nutritional requirements, whose study represents an excellent basis in the field of biotechnology. The transcriptional machinery in Archaea combines the eukaryotic basal apparatus and the bacterial regulation mechanisms. However, little is known about molecular mechanisms of gene expression regulation compared with Bacteria, particularly in Haloarchaea. The genome of Hfx. mediterranei contains a gene, lrp (HFX_RS01210), which encodes a transcriptional factor belonging to Lrp/AsnC family. It is located downstream of the glutamine synthetase gene (HFX_RS01205), an enzyme involved in ammonium assimilation and amino acid metabolism. To study this transcriptional factor more deeply, the lrp gene has been homologously overexpressed and purified under native conditions by two chromatographic steps, namely nickel affinity and gel filtration chromatography, showing that Lrp behaves asa tetrameric protein of approximately 67 kDa. Its promoter region has been characterized under different growth conditions using bgaH as a reporter gene. The amount of Lrp protein was also analyzed by Western blotting in different nitrogen sources and under various stress conditions. To sum up, regarding its involvement in the nitrogen cycle, it has been shown that its expression profile does not change in response to the nitrogen sources tested. Differences in its expression pattern have been observed under different stress conditions, such as in the presence of hydrogen peroxide or heavy metals. According to these results, the Lrp seems to be involved in a general response against stress factors, acting as a first-line transcriptional regulator.
Epigenetic Modulating Chemicals Significantly Affect the Virulence and Genetic Characteristics of the Bacterial Plant Pathogen Xanthomonas campestris pv. campestris
Authors: Baránek Miroslav , Kováčová Viera , Gazdík Filip , Špetík Milan , Eichmeier Aleš , Puławska Joanna , Baránková Kateřina
Published: May 2021

Epigenetics is the study of heritable alterations in phenotypes that are not caused by changes in DNA sequence. In the present study, we characterized the genetic and phenotypic alterations of the bacterial plant pathogen Xanthomonas campestris pv. campestris (Xcc) under different treatments with several epigenetic modulating chemicals. The use of DNA demethylating chemicals unambiguously caused a durable decrease in Xcc bacterial virulence, even after its reisolation from infected plants. The first-time use of chemicals to modify the activity of sirtuins also showed some noticeable results in terms of increasing bacterial virulence, but this effect was not typically stable. Changes in treated strains were also confirmed by using methylation sensitive amplification (MSAP), but with respect to registered SNPs induction, it was necessary to consider their contribution to the observed polymorphism. The molecular basis of the altered virulence was deciphered by using dualRNA-seq analysis of treated Xcc strains infecting Brassica rapa plants. The results of the present study should promote more intensive research in the generally understudied field of bacterial epigenetics, where artificially induced modification by epigenetic modulating chemicals can significantly increase the diversity of bacterial properties and potentially contribute to the further development of the fields, such as bacterial ecology and adaptation.
Assessment of Epidemiological Safety in the Cosmetic Service Industry in Poland: A Cross-Sectional Questionnaire Study
Authors: Gębska-Kuczerowska Anita , Kucharska Izabela , Segiet-Święcicka Agnieszka , Kuczerowski Marcin , Gajda Robert
Published: May 2021

The variety of current cosmetic procedures has increased the potential risks of adverse events and infections. In a nationwide cross-sectional study (2013–2015), we assessed the aspects of infection risk in cosmetic services. An anonymous voluntary questionnaire survey was conducted among 813 employees of cosmetic establishments in Poland. The establishments were selected from a register of service providers. The survey was conducted by employees of the State Sanitary Inspectorate during an audit, and the results showed that cosmetic providers were not fully prepared for risk assessment in terms of occupational exposure or infection transmission. The majority of the respondents (84%) reportedly washed the salon tools. Some establishments did not perform any decontamination (2%) or sterilization (~13%) procedures. Occupational punctures or lacerations occurred from needles, ampoules-syringes, or razors. Most respondents had attended professional training or studied medical textbooks. Approximately 1.7% of the respondents had not updated their knowledge, and 5% gained knowledge from unauthorized sources.The project’s results impacted a variety of innovations and improvements in the field of public health. The results were used to update the national education program (2012–2017); more attention has been directed toward effective education in infection prevention, general hygiene, and post-exposure procedures. Moreover, the study’s results were grounds for the introduction of legislative modifications in the field of epidemiological safety standards for cosmetic services in Poland.
Know-Me: A Toolkit for Designing Personalised Dementia Care
Authors: Wang Gubing , Albayrak Armagan , Hogervorst Eef , van der Cammen Tischa J. M.
Published: May 2021

Personalisation is a crucial element in providing person-centred care for people with dementia. This paper presents the development and evaluation of a design toolkit to facilitate the work of designers and healthcare professionals in personalising dementia care. This toolkit, named “Know-me”, was grounded in the findings of Ergonomics in Aging, Co-design, and Data-enabled Design, derived from literature review and from the field during a four-year doctorate project. “Know-me” was designed to be easily accessible, flexible, and engaging, providing concrete and hands-on guidance for designers and healthcare professionals to use in designing for personalised dementia care. A proof-of-concept evaluation of the “Know-me” toolkit was conducted via student projects on design for dementia care. During this process, we found that “Know-me” could be adapted flexibly so that the care team could use some of the tools by themselves. A feature-by-feature comparison of the “Know-me” toolkit with similar state-of-the-art toolkits was conducted, and based upon this, the strengths and weaknesses of the “Know-me” toolkit are discussed. This preliminary study indicates that the “Know-me” toolkit is a helpful addition to the current pool of toolkits on designing for dementia care.
Optical Redox Imaging of Treatment Responses to Nampt Inhibition and Combination Therapy in Triple-Negative Breast Cancer Cells
Authors: Podsednik Allison , Jiang Jinxia , Jacob Annemarie , Li Lin Z. , Xu He N.
Published: May 2021

We evaluated the utility of optical redox imaging (ORI) to identify the therapeutic response of triple-negative breast cancers (TNBC) under various drug treatments. Cultured HCC1806 and MDA-MB-231 cells treated with FK866 (nicotinamide phosphoribosyltransferase (Nampt) inhibitor), FX11 (lactate dehydrogenase A inhibitor), paclitaxel, and their combinations were subjected to ORI, followed by imaging fluorescently labeled reactive oxygen species (ROS). Cell growth inhibition was measured by a cell viability assay. We found that both cell lines experienced significant NADH decrease and redox ratio (Fp/(NADH+Fp)) increase due to FK866 treatment; however, HCC1806 was much more responsive than MDA-MB-231. We further studied HCC1806 with the main findings: (i) nicotinamide riboside (NR) partially restored NADH in FK866-treated cells; (ii) FX11 induced an over 3-fold NADH increase in FK866 or FK866+NR pretreated cells; (iii) FK866 combined with paclitaxel caused synergistic increases in both Fp and the redox ratio; (iv) FK866 sensitized cells to paclitaxel treatments, which agrees with the redox changes detected by ORI; (v) Fp and the redox ratio positively correlated with cell growth inhibition; and (vi) Fp and NADH positively correlated with ROS level. Our study supports the utility of ORI for detecting the treatment responses of TNBC to Nampt inhibition and the sensitization effects on standard chemotherapeutics.
From Metabolism to Genetics and Vice Versa: The Rising Role of Oncometabolites in Cancer Development and Therapy
Authors: Di Gregorio Emanuela , Miolo Gianmaria , Saorin Asia , Steffan Agostino , Corona Giuseppe
Published: May 2021

Over the last decades, the study of cancer metabolism has returned to the forefront of cancer research and challenged the role of genetics in the understanding of cancer development. One of the major impulses of this new trend came from the discovery of oncometabolites, metabolic intermediates whose abnormal cellular accumulation triggers oncogenic signalling and tumorigenesis. These findings have led to reconsideration and support for the long-forgotten hypothesis of Warburg of altered metabolism as oncogenic driver of cancer and started a novel paradigm whereby mitochondrial metabolites play a pivotal role in malignant transformation. In this review, we describe the evolution of the cancer metabolism research from a historical perspective up to the oncometabolites discovery that spawned the new vision of cancer as a metabolic disease. The oncometabolites’ mechanisms of cellular transformation and their contribution to the development of new targeted cancer therapies together with their drawbacks are further reviewed and discussed.
The CNS/PNS Extracellular Matrix Provides Instructive Guidance Cues to Neural Cells and Neuroregulatory Proteins in Neural Development and Repair
Authors: Melrose James , Hayes Anthony J. , Bix Gregory
Published: May 2021

Background. The extracellular matrix of the PNS/CNS is unusual in that it is dominated by glycosaminoglycans, especially hyaluronan, whose space filling and hydrating properties make essential contributions to the functional properties of this tissue. Hyaluronan has a relatively simple structure but its space-filling properties ensure micro-compartments are maintained in the brain ultrastructure, ensuring ionic niches and gradients are maintained for optimal cellular function. Hyaluronan has cell-instructive, anti-inflammatory properties and forms macro-molecular aggregates with the lectican CS-proteoglycans, forming dense protective perineuronal net structures that provide neural and synaptic plasticity and support cognitive learning. Aims. To highlight the central nervous system/peripheral nervous system (CNS/PNS) and its diverse extracellular and cell-associated proteoglycans that have cell-instructive properties regulating neural repair processes and functional recovery through interactions with cell adhesive molecules, receptors and neuroregulatory proteins. Despite a general lack of stabilising fibrillar collagenous and elastic structures in the CNS/PNS, a sophisticated dynamic extracellular matrix is nevertheless important in tissue form and function. Conclusions. This review provides examples of the sophistication of the CNS/PNS extracellular matrix, showing how it maintains homeostasis and regulates neural repair and regeneration.
Repression of MUC1 Promotes Expansion and Suppressive Function of Myeloid-Derived Suppressor Cells in Pancreatic and Breast Cancer Murine Models
Authors: Sahraei Mahnaz , Bose Mukulika , Sanders J. Alexa , De Chandrav , DasRoy Lopamudra , Nath Sritama , Brouwer Cory R. , Mukherjee Pinku
Published: May 2021

Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells that are responsible for immunosuppression in tumor microenvironment. Here we report the impact of mucin 1 (MUC1), a transmembrane glycoprotein, on proliferation and functional activity of MDSCs. To determine the role of MUC1 in MDSC phenotype, we analyzed MDSCs derived from wild type (WT) and MUC1-knockout (MUC1KO) mice bearing syngeneic pancreatic (KCKO) or breast (C57MG) tumors. We observed enhanced tumor growth of pancreatic and breast tumors in the MUC1KO mice compared to the WT mice. Enhanced tumor growth in the MUC1KO mice was associated with increased numbers of suppressive MDSCs and T regulatory (Tregs) cells in the tumor microenvironment. Compared to the WT host, MUC1KO host showed higher levels of iNOS, ARG1, and TGF-β, thus promoting proliferation of MDSCs with an immature and immune suppressive phenotype. When co-cultured with effector T cells, MDSCs from MUC1KO mice led to higher repression of IL-2 and IFN-γ production by T cells as compared to MDSCs from WT mice. Lastly, MDSCs from MUC1KO mice showed higher levels of c-Myc and activated pSTAT3 as compared to MDSCs from WT mice, suggesting increased survival, proliferation, and prevention of maturation of MDSCs in the MUC1KO host. We report diminished T cell function in the KO versus WT mice. In summary, the data suggest that MUC1 may regulate signaling pathways that are critical to maintain the immunosuppressive properties of MDSCs.
SA-Mediated Regulation and Control of Abiotic Stress Tolerance in Rice
Authors: Nadarajah Kalaivani , Abdul Hamid Nur Wahida , Abdul Rahman Nur Sabrina Natasha
Published: May 2021

Environmental or abiotic stresses are a common threat that remains a constant and common challenge to all plants. These threats whether singular or in combination can have devastating effects on plants. As a semiaquatic plant, rice succumbs to the same threats. Here we systematically look into the involvement of salicylic acid (SA) in the regulation of abiotic stress in rice. Studies have shown that the level of endogenous salicylic acid (SA) is high in rice compared to any other plant species. The reason behind this elevated level and the contribution of this molecule towards abiotic stress management and other underlying mechanisms remains poorly understood in rice. In this review we will address various abiotic stresses that affect the biochemistry and physiology of rice and the role played by SA in its regulation. Further, this review will elucidate the potential mechanisms that control SA-mediated stress tolerance in rice, leading to future prospects and direction for investigation.
Identification of Novel Genomic-Variant Patterns of OR56A5, OR52L1, and CTSD in Retinitis Pigmentosa Patients by Whole-Exome Sequencing
Authors: Lin Ting-Yi , Chang Yun-Chia , Hsiao Yu-Jer , Chien Yueh , Jheng Ying-Chun , Wu Jing-Rong , Ching Lo-Jei , Hwang De-Kuang , Hsu Chih-Chien , Lin Tai-Chi , Chou Yu-Bai , Huang Yi-Ming , Chen Shih-Jen , Yang Yi-Ping , Tsai Ping-Hsing
Published: May 2021

Inherited retinal dystrophies (IRDs) are rare but highly heterogeneous genetic disorders that affect individuals and families worldwide. However, given its wide variability, its analysis of the driver genes for over 50% of the cases remains unexplored. The present study aims to identify novel driver genes, disease-causing variants, and retinitis pigmentosa (RP)-associated pathways. Using family-based whole-exome sequencing (WES) to identify putative RP-causing rare variants, we identified a total of five potentially pathogenic variants located in genes OR56A5, OR52L1, CTSD, PRF1, KBTBD13, and ATP2B4. Of the variants present in all affected individuals, genes OR56A5, OR52L1, CTSD, KBTBD13, and ATP2B4 present as missense mutations, while PRF1 and CTSD present as frameshift variants. Sanger sequencing confirmed the presence of the novel pathogenic variant PRF1 (c.124_128del) that has not been reported previously. More causal-effect or evidence-based studies will be required to elucidate the precise roles of these SNPs in the RP pathogenesis. Taken together, our findings may allow us to explore the risk variants based on the sequencing data and upgrade the existing variant annotation database in Taiwan. It may help detect specific eye diseases such as retinitis pigmentosa in East Asia.
Recombinant Long-Acting Thioredoxin Ameliorates AKI to CKD Transition via Modulating Renal Oxidative Stress and Inflammation
Authors: Nishida Kento , Watanabe Hiroshi , Murata Ryota , Tokumaru Kai , Fujimura Rui , Oshiro Shun , Nagasaki Taisei , Miyahisa Masako , Hiramoto Yuto , Nosaki Hiroto , Imafuku Tadashi , Maeda Hitoshi , Fukagawa Masafumi , Maruyama Toru
Published: May 2021

An effective strategy is highly desirable for preventing acute kidney injury (AKI) to chronic kidney disease (CKD) transition. Thioredoxin-1 (Trx), a redox-active protein that has anti-oxidative and anti-inflammatory properties, would be a candidate for this but its short half-life limits its clinical application. In this study, we examined the renoprotective effect of long-acting Trx that is comprised of human albumin and Trx (HSA-Trx) against AKI to CKD transition. AKI to CKD mice were created by renal ischemia-reperfusion (IR). From day 1 to day 14 after renal IR, the recovery of renal function was accelerated by HSA-Trx administration. On day 14, HSA-Trx reduced renal fibrosis compared with PBS treatment. At the early phase of fibrogenesis (day 7), HSA-Trx treatment suppressed renal oxidative stress, pro-inflammatory cytokine production and macrophage infiltration, thus ameliorating tubular injury and fibrosis. In addition, HSA-Trx treatment inhibited G2/M cell cycle arrest and apoptosis in renal tubular cells. While renal Trx protein levels were decreased after renal IR, the levels were recovered by HSA-Trx treatment. Together, HSA-Trx has potential for use in the treatment of AKI to CKD transition via its effects of modulating oxidative stress and inflammation.
Self-Targeting of Carbon Dots into the Cell Nucleus: Diverse Mechanisms of Toxicity in NIH/3T3 and L929 Cells
Authors: Havrdová Markéta , Urbančič Iztok , Bartoň Tománková Kateřina , Malina Lukáš , Štrancar Janez , Bourlinos Athanasios B.
Published: May 2021

It is important to understand the nanomaterials intracellular trafficking and distribution and investigate their targeting into the nuclear area in the living cells. In our previous study, we firstly observed penetration of nonmodified positively charged carbon dots decorated with quaternary ammonium groups (QCDs) into the nucleus of mouse NIH/3T3 fibroblasts. Thus, in this work, we focused on deeper study of QCDs distribution inside two healthy mouse NIH/3T3 and L929 cell lines by fluorescence microspectroscopy and performed a comprehensive cytotoxic and DNA damage measurements. Real-time penetration of QCDs across the plasma cell membrane was recorded, concentration dependent uptake was determined and endocytic pathways were characterized. We found out that the QCDs concentration of 200 µg/mL is close to saturation and subsequently, NIH/3T3 had a different cell cycle profile, however, no significant changes in viability (not even in the case with QCDs in the nuclei) and DNA damage. In the case of L929, the presence of QCDs in the nucleus evoked a cellular death. Intranuclear environment of NIH/3T3 cells affected fluorescent properties of QCDs and evoked fluorescence blue shifts. Studying the intracellular interactions with CDs is essential for development of future applications such as DNA sensing, because CDs as DNA probes have not yet been developed.
Ionic Liquids—A Review of Their Toxicity to Living Organisms
Authors: Gonçalves Ana R.P. , Paredes Xavier , Cristino A. F. , Santos F. J.V. , Queirós Carla S.G.P.
Published: May 2021

Ionic liquids (ILs) were initially hailed as a green alternative to traditional solvents because of their almost non-existent vapor pressure as ecological replacement of most common volatile solvents in industrial processes for their damaging effects on the environment. It is common knowledge that they are not as green as desired, and more thought must be put into the biological consequences of their industrial use. Still, compared to the amount of research studying their physicochemical properties and potential applications in different areas, there is a scarcity of scientific papers regarding how these substances interact with different organisms. The intent of this review was to compile the information published in this area since 2015 to allow the reader to better understand how, for example, bacteria, plants, fish, etc., react to the presence of this family of liquids. In general, lipophilicity is one of the main drivers of toxicity and thus the type of cation. The anion tends to play a minor (but not negligible) role, but more research is needed since, owing to the very nature of ILs, except for the most common ones (imidazolium and ammonium-based), many of them are subject to only one or two articles.
Bayesian Network Analysis of Lysine Biosynthesis Pathway in Rice
Authors: Lahiri Aditya , Rastogi Khushboo , Datta Aniruddha , Septiningsih Endang M.
Published: May 2021

Lysine is the first limiting essential amino acid in rice because it is present in the lowest quantity compared to all the other amino acids. Amino acids are the building block of proteins and play an essential role in maintaining the human body’s healthy functioning. Rice is a staple food for more than half of the global population; thus, increasing the lysine content in rice will help improve global health. In this paper, we studied the lysine biosynthesis pathway in rice (Oryza sativa) to identify the regulators of the lysine reporter gene LYSA (LOC_Os02g24354). Genetically intervening at the regulators has the potential to increase the overall lysine content in rice. We modeled the lysine biosynthesis pathway in rice seedlings under normal and saline (NaCl) stress conditions using Bayesian networks. We estimated the model parameters using experimental data and identified the gene DAPF(LOC_Os12g37960) as a positive regulator of the lysine reporter gene LYSA under both normal and saline stress conditions. Based on this analysis, we conclude that the gene DAPF is a potent candidate for genetic intervention. Upregulating DAPF using methods such as CRISPR-Cas9 gene editing strategy has the potential to upregulate the lysine reporter gene LYSA and increase the overall lysine content in rice.
Identification of Mode Shapes of a Composite Cylinder Using Convolutional Neural Networks
Authors: Miller Bartosz , Ziemiański Leonard
Published: May 2021

The aim of the following paper is to discuss a newly developed approach for the identification of vibration mode shapes of multilayer composite structures. To overcome the limitations of the approaches based on image analysis (two-dimensional structures, high spatial resolution of mode shapes description), convolutional neural networks (CNNs) are applied to create a three-dimensional mode shapes identification algorithm with a significantly reduced number of mode shape vector coordinates. The CNN-based procedure is accurate, effective, and robust to noisy input data. The appearance of local damage is not an obstacle. The change of the material and the occurrence of local material degradation do not affect the accuracy of the method. Moreover, the application of the proposed identification method allows identifying the material degradation occurrence.
Microstructure and Abrasive Wear Resistance of Metal Matrix Composite Coatings Deposited on Steel Grade AISI 4715 by Powder Plasma Transferred Arc Welding Part 2. Mechanical and Structural Properties of a Nickel-Based Alloy Surface Layer Reinforced with Particles of Tungsten Carbide and Synthetic Metal–Diamond Composite
Authors: Czupryński Artur
Published: May 2021

The article is the continuation of a cycle of works published in a Special Issue of MDPI entitled “Innovative Technologies and Materials for the Production of Mechanical, Thermal and Corrosion Wear-Resistant Surface Layers and Coatings” related to tests concerning the microstructure and mechanical properties of innovative surface layers made using the Powder Plasma Transferred Arc Welding (PPTAW) method and intended for work surfaces of drilling tools and machinery applied in the extraction industry. A layer subjected to tests was a metal matrix composite, made using powder based on a nickel alloy containing spherical fused tungsten carbide (SFTC) particles, which are fused tungsten carbide (FTC) particles and spherical particles of tungsten-coated synthetic metal–diamond composite (PD-W). The layer was deposited on the substrate of low-alloy structural steel grade AISI 4715. The results showed that the chemical composition of the metallic powder as well as the content of the hard phase constituting the matrix enabled the making of a powder filler material characterised by very good weldability and appropriate melting. It was also found that the structure of the Ni-WC-PD-W layer was complex and that proper claddings (characterised by the uniform distribution of tungsten carbide (WC)) were formed in relation to specific cladding process parameters. In addition, the structure of the composite layer revealed the partial thermal and structural decomposition of tungsten carbide, while the particles of the synthetic metal–diamond composite remained coherent. The deposited surface layer was characterised by favourable resistance to moderate dynamic impact loads with a potential energy of 200 J, yet at the same time, by over 12 times lower metal–mineral abrasive wear resistance than the previously tested surface layer made of cobalt-based composite powder, the matrix of which contained the hard phase composed of TiC particles and synthetic metal–diamond composite. The lower abrasive wear resistance could result from a different mechanism responsible for the hardening of the spherical particles of the hard phase susceptible to separation from the metal matrix, as well as from a different mechanism of tribological wear.
The Influence of Yarn and Weave Structures on Acoustic Materials and the Effect of Different Acoustic Signal Incidence Angles on Woven Fabric Absorption Possibilities
Authors: Samuel Bethalihem Teferi , Barburski Marcin , Blaszczak Jaroslaw R , Witczak Ewa , Abramczyk Katarzyna
Published: May 2021

Utilizing textile-based acoustic materials can be considered basically from two points of view. First, it may be used as a sound absorbing material. Second, it may be used as a decoration that gives the surrounding area a new artistic appearance. To improve the acoustic possibilities of any woven fabric, it is necessary to study the influences of yarn characteristics and the internal structures of weave interlacement. To understand the impact of the yarn on the fabric, the samples were prepared using only polyester fiber as textured, twisted, and staple yarns. Regarding this experiment, the basic weave’s structure type, such as plain, rib, sateen, and twill, were used. Overall, 16 woven fabrics were prepared. The investigation was performed in the range of low to medium acoustic frequencies. The experiments were conducted in an anechoic chamber. Compared to other yarn types, fabrics formed from textured polyester yarn had higher sound absorption properties. Moreover, the observed results show that the different incidence angles of acoustic signals influence the measured sound absorption properties of a textile.
The Effect of the Reaction pH on Properties of Lead(II) Azide
Authors: Biegańska Jolanta
Published: May 2021

Lead(II) azide is an initiating explosive; even a small amount can trigger an explosion caused by simple external stimuli, such as sparks, flames, friction or pinpricks, and is able to initiate the explosive reaction of rock-crushing explosives. Due to the fact that this initiating explosive triggers further reactions, the effect of priming detonators depends on the properties of its material. Its sensitivity is associated with the size of its crystals. For instance, it is used for mining detonators in the form of fine crystals. The quality of the crystals is also correlated to the safety of the production process, i.e., the crystals should be round-shaped rather than needle-like since breaking it would inevitably trigger an explosion. The process of lead(II) azide production on an industrial scale is based on the reaction of lead(II) nitrate with sodium azide with the presence of dextrin, which determines the desired shape of the crystals. The reaction pH affects the number of sediment particles formed in a periodical reactor. Changing the pH from 6.5 to 7.5 leads to the rapid growth of crystal particles.
Preparation and Performance Evaluation of Platinum Barium Hexaaluminate Catalyst for Green Propellant Hydroxylamine Nitrate Thrusters
Authors: Kang Shinjae , Kwon Sejin
Published: May 2021

Spacecraft have monopropellant thruster systems for attitude control in the vacuum of space. Hydroxylamine nitrate is a green propellant that has high performance and low toxicity. Owing to the high adiabatic decomposition temperature of the hydroxylamine nitrate propellant, it is necessary to develop a catalyst with high thermal stability. We used a platinum barium hexaaluminate catalyst for green propellant hydroxylamine nitrate thrusters. Barium hexaaluminate support was prepared by a wet impregnation method and heat treatment. Platinum, the active material, was coated on catalyst supports. The Brunauer–Emmett–Teller specific surface was also investigated. X-ray diffraction and scanning electron microscope imagery were used to confirm the formation of barium hexaaluminate. A hydroxylamine nitrate propellant blended with methanol was used for performance evaluation via firing tests of the thruster. The catalytic decomposition performance of each test was evaluated by calculating the characteristic velocity efficiency using the pressure of the chamber at the end of the catalyst bed and the mass flow rate of the propellant. As the catalyst bed was preheated to 350 °C, the characteristic velocity efficiency was 71.9%. Test results revealed that the platinum barium hexaaluminate catalyst is feasible for a hydroxylamine nitrate thruster.
Tetrodotoxins (TTXs) and Vibrio alginolyticus in Mussels from Central Adriatic Sea (Italy): Are They Closely Related?
Authors: Bacchiocchi Simone , Campacci Debora , Siracusa Melania , Dubbini Alessandra , Leoni Francesca , Tavoloni Tamara , Accoroni Stefano , Gorbi Stefania , Giuliani Maria Elisa , Stramenga Arianna , Piersanti Arianna
Published: May 2021

Tetrodotoxins (TTXs), potent neurotoxins, have become an increasing concern in Europe in recent decades, especially because of their presence in mollusks. The European Food Safety Authority published a Scientific Opinion setting a recommended threshold for TTX in mollusks of 44 µg equivalent kg−1 and calling all member states to contribute to an effort to gather data in order to produce a more exhaustive risk assessment. The objective of this work was to assess TTX levels in wild and farmed mussels (Mytilus galloprovincialis) harvested in 2018–2019 along the coastal area of the Marche region in the Central Adriatic Sea (Italy). The presence of Vibrio spp. carrying the non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes, which are suspected to be involved in TTX biosynthesis, was also investigated. Out of 158 mussel samples analyzed by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS), 11 (7%) contained the toxins at detectable levels (8–26 µg kg−1) and 3 (2%) contained levels above the EFSA safety threshold (61–76 µg kg−1). Contaminated mussels were all harvested from natural beds in spring or summer. Of the 2019 samples, 70% of them contained V. alginolyticus strains with the NRPS and/or PKS genes. None of the strains containing NRPS and/or PKS genes showed detectable levels of TTXs. TTXs in mussels are not yet a threat in the Marche region nor in Europe, but further investigations are surely needed.