bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGARUH OPINI AUDIT, REPORTING DELAY, PROFITABILITAS, SOLVABILITAS, DAN LIKUIDITAS TERHADAP HARGA SAHAM
Authors: Herlambang Rizky
Published: December 2024

This study aims to examine the effect of Audit Opinion, Reporting Delay, Profitability, Solvency, and Liquidity on Stock Prices. The technique of taking samples using purposive sampling method. The data used in this study is secondary data in the form of annual financial reports from 32 banking companies listed on the Indonesia Stock Exchange (IDX) during the period 2019 – 2021. Data analysis was carried out using the SPSS application version 25 for windows. The results of the T-TEST showed that the significance value of the audit opinion variable was 0.468, the significance value of the reporting delay variable was 0.840, the significance value of the profitability variable was 0.000, the significance value of the solvency variable was 0.543, the significance value of the liquidity variable was 0.011. Then the results of the F TEST obtain a probability value of 0.001 which is smaller than the significance value of 0.05. Based on these results it can be concluded that, partially, the profitability variable has a significant positive effect on stock prices and the liquidity variable has a significant negative effect on stock prices. while for the variable audit opinion, reporting delay, and solvency, have no significant effect on stock prices. Then simultaneously concluded that the variables of audit opinion, reporting delay, profitability, solvency, and liquidity together have a significant effect on stock prices.
MANAJEMEN RISIKO KREDIT GUNA MEMINIMALISIR KREDIT BERMASALAH PADA PERUMDA BPR KABUPATEN MADIUN
Authors: Hermawan Hery , Rochman Fatchur , Iswati Retno
Published: December 2024

Abstract— This study aims to determine the credit management carried out at Perumda BPR Madiun Regency in an effort to minimize non-performing loans, to find out what factors cause non-performing loans to Perumda BPR Madiun Regency. On the other hand, it is to find out the handling of non-performing loans at Perumda BPR Madiun Regency. This type of research is a descriptive research. Data obtained from primary data, namely data obtained from informants directly and secondary data in the form of documents or previous research. Data collection techniques namely interviews, observation and review of written documents.
The results of the study show that credit management carried out at Perumda BPR Madiun Regency in an effort to minimize non-performing loans has been able to run effectively. This is because the implementation of credit management carried out at Perumda BPR Madiun Regency in an effort to minimize non-performing loans is in accordance with POJK No. 13/POJK.03/2015. As well as the factors that cause problem loans, namely internal factors originating from the Perumda BPR Madiun Regency and external factors originating from the debtor. In addition, the handling of non-performing loans at Perumda BPR Madiun Regency takes the form of rescheduling, reconditioning and restructuring.
PENGARUH KEPEMILIKAN NPWP, PEMERIKSAAN PAJAK, PENAGIHAN PAJAK, DAN KESADARAN WAJIB PAJAK TERHADAP PENERIMAAN PAJAK PENGHASILAN
Authors: Saputra Dimas Syahrul , Rochman Fatchur , Purnomo Herry
Published: December 2024

This study aims to analyze the effect of NPWP Ownership, Tax Audit, Tax Collection and Taxpayer Awareness in increasing Income Tax Revenue. This study uses Quantitative Descriptive research methods. The location of this research and the respondents in this study were offices and employees with a sample of 70 respondents who had NPWP in Sidorejo District, Magetan Regency. The results of hypothesis research show that the independent variable of NPWP ownership is 0.001, the independent variable of tax audit is 0.049, the independent variable of tax collection is 0.040 and the independent variable of taxpayer awareness is 0.041. With these results it can be stated that NPWP ownership, tax audit, tax collection, and taxpayer awareness have a significant effect. Simultaneously, the variables of NPWP ownership, tax audit, tax collection and taxpayer awareness affect the income tax revenue variable.
Pengaruh Service Quality, Social Media Marketing Dan Customer Engagement Terhadap Keputusan Nasabah
Authors: Jun Monica , Mulyati Tatik , Imron Muhammad
Published: December 2024

This research aims to determine the influence of service quality, social media marketing and customer engagement partially and simultaneously on customer decisions at PT Pegadaian (Persero) Magetan Branch. This research uses a quantitative approach in associative form. The data sources used are primary data and secondary data obtained directly from respondents. The population in this research were 200 customers at PT Pegadaian (Persero) Magetan Branch and the sample size used was 133 customers. The sampling technique used probability sampling technique. Data analysis was carried out using multiple linear regression with the SPSS version 25.00 for Windows program. The research results show that the independent variable service quality has a calculated t value of 3.818, the independent social media marketing variable has a calculated t value of 2.148 and the independent variable customer engagement has a calculated t value of 1.567 where the t table value for each variable is 1.710. So, partially, 1) service quality and social media marketing influence customer decisions. 2) But customer engagement has no effect on customer decisions. 3) Simultaneously, service quality, social media marketing and customer engagement influence customer decisions. 4) Service Quality is the dominant variable in influencing customer decisions.
Peran Kepuasan Konsumen sebagai Pemediasi Promosi Sosial Media dan Diskon terhadap Loyalitas Konsumen
Authors: Khairunnisa Thifal , Fajri Annisa , Haerudin
Published: December 2024

Generation Z is a potential consumer for e-commerce, because this generation grew up with the internet and uses it every day, Generation Z is very familiar with it and believes that this is a very important medium. So, it is important to create loyal consumers which is the core of every business and for companies loyalty is very important. In this research, we use the role of consumer satisfaction as a mediator of consumer loyalty towards social media promotions and discounts. The object of this research is Management Study Program students at Muhammadiyah University Semarang who have made purchases on Shopee e-commerce at least 3 times. Sampling using incidental sampling is a technique used to determine samples based on anyone who happens to fill out a Google form. The results of this research are that there is a mediating role for consumer satisfaction to increase consumer loyalty through social media promotions and discounts.
Sistem Informasi Rekam Medis Klinik Kasih Murni Berbasis web
Authors: Ilham Anjis Ilham Rahmatdani
Published: December 2024

In the midst of the rapid advancement of information and communication technology, fast and accurate information is increasingly becoming the main need for decision makers. In other words, information is a basic requirement required by every management to make decisions. Even a hospital as an institution that stores a lot of data also requires correct and accurate data processing that can be presented in such a way in the form of a report. Presentation of reports in the form of information must be in accordance with the value of the use and function of each part. One of them is a web-based medical record information system. In this study, the design and analysis techniques in the manufacture used the Waterfall method. This system has been tested using black box testing to get the expected results and the system is feasible to use. Based on the results of the description and discussion of the final project report, a Medical Record Information System has been successfully built in the Kasih Murni clinic for monitoring health in Website-Based Children using the waterfall method and the code igniter framework with the PHP programming language. The system is expected to be able to assist in processing medical records in clinics and hospitals where as long as the recording of medical record data is still done manually.
Pengaruh Bioetanol Terhadap Konsumsi Bahan Bakar Dan Emisi Gas Buang Pada Motor Suzuki Shogun 125 CC
Authors: Mohammad Aldo Azis
Published: December 2024

The octane value of motor vehicle fuel has a very big influence on the combustion process in the cylinder space. Apart from that, the speed of the combustion reaction is also determined by the octane value of the fuel. One of the factors that influences the performance of motorized vehicles is the quality of the fuel used. Bioethanol is a fuel derived from organic materials which is processed into a renewable additive that is cost effective and environmentally friendly. The function of bioethanol is to increase the octane content of fuel to increase fuel efficiency, apart from that, it is also an oxygenating agent, containing O2 which can help maximize combustion and reduce air pollution. This research aims to find out the effect of bioethanol on fuel consumption and exhaust emissions on the Suzuki Shogun 125 CC motorbike. This research uses the experimental method, namely by testing the effect of an experiment by comparing a new experiment with a control or comparison treatment. The experiment was the effect of Bioethanol with Pertalite on fuel consumption and exhaust emissions on a Suzuki Shogun 125 CC motorbike which was measured using a burret and gas analyser. After carrying out research, data on fuel consumption for the E0 fuel mixture had a consumption result of 3.7666 ml/minute and the E2% mixture had a consumption result of 2.9333 ml/minute, experiencing a decrease in fuel consumption of 0.8333 ml/minute. The decrease in CO content which was originally in the E0 mixture was 6.08%, becoming the lowest CO among the other mixtures in the E2% mixture with a CO content of 5.60% which experienced a decrease of 0.48%, and in the HC content of the E0 mixture with a value of 387ppm became lower in the E2% fuel mixture with a content of 344ppm, decreasing by 43 ppm.
Sistem Pemberian Pakan Ikan Terotomatisasi Berbasis IoT
Authors: Siti Nur Kholisah
Published: December 2024

Salah satu pemanfaatan teknologi Internet of Things yang sering disebut IoT adalah untuk membantu pekerjaan manusia, terutama pekerjaan yang membutuhkan energi. Karena teknologi ini dapat menghubungkan objek-objek di dunia nyata untuk saling berkomunikasi sebagai bagian dari satu sistem yang terpadu dengan menggunakan jaringan internet sebagai penghubungnya, penelitian ini dilakukan karena adanya permasalahan pada pemberian pakan ikan yang berdampak pada hasil panen para pembudidaya ikan menjadi menurun. Oleh karena itu, penulis membuat alat bantu pemberian pakan ikan untuk membantu para pembudidaya ikan dalam pemberian pakan ikan baik secara manual maupun otomatis yang berbasis IoT, agar pembudidaya dapat memonitor dan mengontrol pemberian pakan melalui website. Metode yang digunakan dalam perancangan sistem adalah Waterfall. Metode pengujian menguji fungsionalitas perangkat lunak aplikasi, dengan menggunakan pengujian Blackbox. Alat bantu pemberian pakan ikan yang memanfaatkan Internet of Things (IoT) telah berhasil dibangun. Berdasarkan hasil pengujian black box, diperoleh kesimpulan bahwa sistem berfungsi dengan baik sesuai dengan waktu terjadwal yang ditentukan.
Pengaruh Penambahan Serbuk Alumunium Sebagai Bahan Pengisi Terhadap Kuat Tekan Beton
Authors: Muhamad Nurwahidin Erdiansyah
Published: December 2024

Serbuk alumunium merupakan limbah hasil pemotongan alumunium yang diolah lagi hingga menjadi butiran kecil berupa serbuk dengan ukuran 200 sampai 320 mesh. Alumunium merupakan material yang memiliki karakteristik tahan korosi, tahan panas, tidak berkarat dan termasuk bahan ringan, juga kuat. Sehingga banyak digunakan dalam kehidupan sehari-hari. Karena alumunium merupakan bahan yang tidak dapat terurai alumunium bisa jadi alternatif untuk dijadikan bahan tambah dalam pembuatan beton. Alumunium yang digunakan berupa serbuk dengan ukuran diatas 200 mesh. Karena ukuran yang melebihi 200 mesh serbuk alumunium ini digunakan sebagai bahan pengisi atau filer pada beton. Pada penelitian ini menggunakan metode eksperimen dengan benda uji silinder 15x30 cm dengan total benda uji 12 buah, dalam penelitian ini variasi serbuk alumunium sebesar 0%, 0,3%, 0,5%, 0,7% dari hasil pengujian kuat tekan diketahui pada variasi 0% kuat tekan sebesar 18,48 MPa, persentase 0,3% sebesar 10,37 MPa, persentase 0,5% sebesar 9,62 MPa, persentase 0,7% sebesar 9,24. Dari hasil pengujian diperoleh nilai porositas pada beton variasi serbuk alumunium persentase 0% sebesar 0.98%, persentase 0,3% sebesar 2,19%, persentase 0,5% sebesar 2,69%, persentase 0,7% sebesar 3,14%.
Mengungkap Batasan Kecakapan Akademik: Penyelidikan Kritis terhadap Perfeksionisme Sains di Kalangan Sarjana Pendidikan Biologi
Authors: Aini Mutia Desti , Ferry Dharma , Damni Albertos
Published: December 2024

Beberapa penelitian sebelumnya mengungkapkan bahwa banyak siswa yang terjebak dalam siklus perfeksionisme akademik. Penelitian ini menggunakan metode campuran untuk mengatasi keterbatasan penelitian kuantitatif atau kualitatif murni yang didorong oleh ideologi. Penelitian difokuskan pada 131 mahasiswa aktif biologi pada tahun ajaran 2023/2024. Teknik total sampling pada awalnya digunakan, namun karena data outlier yang ekstrim dan kuesioner yang tidak terkembalikan, jumlah akhir sampel mnjadi 100 mahasiswa biologi. Pengumpulan data diawali dengan penyebaran kuesioner penilaian perfeksionisme akademik, dilanjutkan dengan analisis kuantitatif menggunakan aplikasi JASP untuk mengungkap hubungan berbagai variabel demografi dan perfeksionisme akademik pada mahasiswa pendidikan biologi. Selanjutnya, wawancara dilakukan terhadap beberapa mahasiswa untuk mengkonfirmasi dan mendalami pengalaman mereka terkait dengan topik penelitian. Temuan penelitian ini menunjukkan bahwa meskipun sebagian besar siswa biologi cenderung menunjukkan perfeksionisme akademik, terdapat juga variasi individu yang signifikan dalam sifat ini. Penelitian ini menggarisbawahi pentingnya pemahaman perfeksionisme akademik pada mahasiswa pendidikan biologi, untuk pengembangan pendidikan dan kesadaran kesehatan mental, karena perfeksionisme akademik rentan terhadap stres, kecemasan, dan depresi.
Urgensi Mempelajari Mutasi Dan Variasi Genetik Berorientasi Content Representation Untuk Memahami Evolusi OrganismeAdanya miskonsepsi dan kesulitan siswa dalam pembelajaran materi mutasi menyebabkan pemahaman dan hasil belajar siswa mengalami penurunan.
Authors: Rizqina Mahda , Najira Najira , Hidayat Topik
Published: December 2024

Adanya miskonsepsi dan kesulitan siswa dalam pembelajaran materi mutasi menyebabkan pemahaman dan hasil belajar siswa mengalami penurunan. Peran guru sangat penting dalam memfasilitasi pembelajaran siswa. Guru seharusnya dapat merancang dan memanfaatkan model, metode, dan media yang tepat agar pembelajaran dapat berlangsung secara efektif, utamanya dalam konsep materi yang abstrak dan prosesnya tidak dapat diamati secara langsung seperti pada konsep mutasi dan evolusi.Tujuan pada penelitian ini untuk memetakkan konsep-konsep penting pada pembelajaran mutasi dan variasi genetik berupa kompleksitas dan keabstrakkan untuk memahami materi evolusi Organisme. Penelitian ini menggunakan metode studi literatur. Setelah dilakukan pengkajian pada berbagai literatur, didapatkan identifikasi dan analisis mengenai konsep mutasi dan variasi genetik. sehingga, nantinya diharapkan dapat dijadikan sebagai referensi bagi guru dalam merancang pembelajaran. Terdapat Ide besar diantaranya faktor penyebab mutasi, jenis mutasi, dampak mutasi dan hubungan antara variasi genetik, mutasi, spesiasi, dan biodiversitas yang muncul dalam CoRe menjadi beberapa pertimbangan yang penting dalam konseptualisasi suatu topik saat mengajarkan pada siswa dengan adanya kedalaman dan keluasan materi, guru masih dapat memangkas materi yang disesuaikan dengan kondisi lingkungan belajar siswa. Pembelajaran harus mempertimbangkan kondisi siswa dan guru sehingga penggunaan pendekatan, model, media, dan metode sangat menentukan keberhasilan dalam mengajarkan pelajaran evolusi organisme yang bersifat abstrak ini.
Pendekatan Problem Solving dan Model Project-Based Learning dalam Pembelajaran Hybrid
Authors: Alonemarera Alonemarera , Tendrita Miswandi
Published: December 2024

Pelatihan pengembangan keterampilan berpikir kritis dan komunikasi melalui praktik pembelajaran di lingkup prodi pendidikan biologi Universitas Sembilanbelas November Kolaka selama ini dinilai belum optimal. Jika selama ini prodi dalam membangun keterampilan berpikir kritis dan komunikasi mahasiswa hanya melalui praktik pembelajaran yang terbatas pada lingkungan kelas, maka selanjutnya dapat diperluas dengan memberi akses kepada mahasiswa agar dapat berinteraksi langsung dengan masyarakat luar secara nyata melalui penerapan pendekatan problem solving dipadu project-based learning dengan konsep hybrid learning sebagai wujud pelibatan teknologi yang mendukung konsep merdeka belajar. Oleh karena itu, dilakukan penelitian dengan tujuan mengetahui pengaruh penerapan pendekatan problem solving dipadu project-based learning terhadap keterampilan berpikir kritis dan komunikasi mahasiswa dalam metode pembelajaran hybrid. Penelitian ini berjenis pre-experimental menggunakan one-group pretest-posttest design dan melibatkan 12 orang mahasiswa. Melalui teknik observasi, data penelitian dikumpulkan menggunakan instrumen lembar observasi keterampilan berpikir kritis dan komunikasi. Dari hasil uji paired sample t-test diperoleh nilai Sig. 0.000. Sedangkan uji N-Gain menunjukkan nilai 0.68 untuk keterampilan berpikir kritis dan 0.67 untuk keterampilan komunikasi. Dengan demikian, terdapat pengaruh penerapan pendekatan problem solving dipadu project-based learning dalam pembelajaran hybrid terhadap peningkatan keterampilan berpikir kritis dan komunikasi mahasiswa dimana efektifitas perlakuan berada pada kategori “sedang”.
Implementasi Pembelajaran Higher Order Thinking Menggunakan PhET Interactive Simulation Pada Mata Kuliah Evolusi Untuk Meningkatkan Hasil Belajar Kognitif Mahasiswa
Authors: Husein Ahmad Saddam , Jumiarni Dewi , Hidayat Syarif , Abas Abas
Published: December 2024

PhET Interactive Simulation merupakan simulasi yang dapat memvisualisasi materi pembelajaran, mampu melibatkan mahasiswa secara aktif dalam proses berpikir dan mengambil kesimpulan. Penelitian ini bertujuan untuk mengetahui hasil belajar mahasiswa semester 5 Pendidikan Biologi Universitas Bengkulu pada Mata Kuliah Evolusi serta untuk mengetahui pengaruh pembelajaran Higher Order Thinking menggunakan PhET Interactive Simulation terhadap peningkatan hasil belajar mahasiswa. Penelitian ini menggunakan metode kuasi eksperimen atau eksperimen semu, model Nonequivalent control group design dengan total sampling. Adapun kelas eksperimen pada penelitian ini ialah kelas 5A dengan kelas 5B sebagai kelas kontrol. Instrumen penelitian yang digunakan berupa lembar posttest uraian dan analisis. Sementara itu, metode pembelajaran dikedua kelas disamakan yakni menggunakan diskusi kelompok. Setelah dilakukan pembelajaran, diberikan lembar posttest untuk mengetahui peningkatan pemahaman dan hasil belajar mahasiswa pada Mata Kuliah Evolusi. Sementara itu, model pembelajaran kedua kelas disamakan yakni Higher Order Thinking. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan signifikan pada nilai rata-rata pretest kelas kontrol dengan kelas eksperimen, yakni secara berurutan sebesar 39,71 dan 39,41. Begitu pula setelah pembelajaran, tidak terdapat perbedaan signifikan pada nilai rata-rata posttest kelas kontrol dan eksperimen yakni secara berurutan sebesar 82,94 dan 85,29. Selain itu, penggunaan PhET Interactive Simulation dikategorikan tinggi dengan nilai N-Gain sebesar 0,79 serta penggunaannya dikategorikan efektif dengan nilai N-Gain pretest dan posttest sebesar 79%.
NEWLY DISCOVERED ARCHAEOLOGICAL SITES OF THE CENTRAL-WESTERN PART OF LVIV REGION
Authors: PETEHYRYCH Volodymyr , PAVLIV Dmytro
Published: December 2024

The results of the 2011 archaeological survey of the central-western part of the Lviv region within the former Horodok (now Lviv) district are presented. The natural and geographical features of the studied microregion are characterized, the main types of archaeological sites are highlighted, and the previous history of their study is summarized.

A catalog of newly discovered and surveyed settlements, barrows, hillforts, and locations was compiled, and was supplemented with numerous illustrative materials. At the end of the catalog, a unique find from 2014 on the eastern outskirts of Horodok is analyzed in detail – a burial complex with a rich collection of ceramics and copper products of the Lublin-Volhynian culture, possibly attributable to the cremation burial of one of the representatives of the elite of that time.

According to the results of the research conducted by the method of continuous archaeological survey of selected territorial zones, an area of more than 120 km2 was studied. Seventy-two archeological sites were identified, examined, and documented: 37 settlements, 25 barrow cemeteries (36 barrows), ground burial, hillfort, and 8 separate locations. All of them are presented on a combined map, localized in more detail on separate maps, and recorded in photos. Artifacts collected from the surfaces are presented in drawings and photos. Special attention is paid to the settlements of the Early Middle Ages, recorded near modern localities, the ancient names (ethnonyms) of which are connected with certain historical ethnic groups (Uhry, Uhertsi, Yatvyagi) or represent the oldest Slavic topography (Putyatychi, Rodatychi, Dubanevychi, Lisnovychi, etc. ).

Taking into account all the studies of the central-western part of the Lviv region, it has been established that in this territory we have traces of human settlement and activity from the Stone Age – the Mesolithic to the Late Middle Ages. It is noted that the archaeological sources obtained in a relatively small area indicate that this terrain, like the neighboring geographical areas, was permanently inhabited or used by humans during this time, without major interruptions. It was established that the ancient history and culture of the Horodok area cannot yet be completely reconstructed based on archaeological materials and separate written reports of the annals, but they are a reliable basis for further research and open good prospects for such research.

Keywords: archaeological surveys, the central-western part of Lviv region, archaeological sites of different periods.
Cardiac rhythm and conduction disturbances in children with bradyarrhythmias during long-term ECG monitoring
Authors: Polyakova E. B. , Trofimova T. A. , Shkolnikova M. A.
Published: December 2024

The syncope in children with bradyarrhythmias can be life-threatening and their differentiation is important in the evaluation of any child with syncope. In children risk factors for the poor prognosis and progression of bradyarrhythmias leading to the development of arrhythmogenic syncope have not been definitively determined. The aim was to determine the diagnostic significance of the implantable cardiac monitors (ICM) method and identify electrocardiographic and electrophysiological predictors of the development of syncope in children with bradyarrhythmias. Material and methods. The authors analyze the group of 191 children with bradyarrhythmias, recurrent syncope and ICM. The examination of patients included electrocardiography (ECG), echocardiography (ECHO-CG), Holter monitoring (HM) of rhythm, electrophysiological study (EHS), exercise test, and analysis of family anamnesis data. Results. 96 out of 191 children had positive result of long-term cardiac monitoring. 16 out of 96 children had structural heart deseases – congenital heart defects, cardiomyopathies. Episodes of arrhythmias were documented in 30% of children during syncope and asymptomatic arrhythmias were observed in 43% of children. Arrhythmogenic syncope accounts for 7,5% of all registered syncope and 5% of positive monitoring results. Sinus rhythm during syncope was registered in 48% of children, and 2% of patients had a combination of arrhythmogenic and non-arrhythmogenic syncope. The heart rhythm characteristics of standard ECG and HM in patients with symptomatic arrhythmias did not differ significantly from either patients with asymptomatic episodes of arrhythmias or patients with sinus rhythm during syncope. Conclusion. The diagnostic significance of the ICM for identifying the cause of recurrent syncope in children with bradyarrhythmias is 50%. Arrhythmogenic genesis of syncope has been confirmed in 7.5% of children with bradyarrhythmias. The heart rate characteristics and electrophysiological parameters of the previous examination are not predictors of the development of syncope in children with bradyarrhythmias. Long-term cardiac monitoring of ICM can identify children with life-threatening arrhythmogenic syncope from all patients with bradyarrhythmia and make the prevention of sudden cardiac death (SCD).
Ralstonia pseudosolanacearum Infecting Pumpkin (Cucurbita Maxima) and its Pathogenicity on Cucurbitaceae Family
Authors: Akarapisan Angsana , Kumvinit Athidtaya , Thungrabeab Malee , Thumdee Siwaporn , Kositratana Wichai
Published: December 2024

Ralstonia solanacearum species complex (RSSC) is a devastating phytopathogen with wide host range in many economically important crops all over the world. Bacterial wilt on a pumpkin (Cucurbita maxima) caused by Ralstonia pseudosolanacearum was observed in Chiang Rai, Thailand. The symptoms that appeared were wilting, drooping of half leaves, and oozing from crosscut stems of these diseased plants. The R. pseudosolanacearum-specific 280 bp amplicon, and sequevar 17 confirmed by sequencing the hrpB gene. The hrpB gene have been shown to be key determinants for pathogenicity in the phytopathogenic bacterial wilt. The susceptibility of different Cucurbitaceae species to bacterial wilt were tested. Members of the Cucurbita, Cucumis, Citrullus, Momordica, and Lagenaria genera were susceptible to bacterial wilt, with disease incidence ranging from 30% to 100%. The susceptibility of different Cucurbitaceae species to bacterial wilt, which can provide valuable information for the plant breeding programs to the improvement of pathogen-targeted.
Diatopic variation in four B1 Spanish as a foreign language student’s books
Authors: Almau Almau Sonia
Published: December 2024

This paper examines four Spanish as a Foreign Language (SFL) B1 level textbooks from different publishers based on the central-northern peninsular standard, with the aim of investigating the inclusion of diatopic variation in them. For this purpose, a quantitative analysis was carried out in which, based on the number of exercises in each textbook, the percentage of linguistic features from varieties other than central-northern peninsular is measured. Additionally, the percentage of these features is calculated by linguistic level (phonetic-phonological, morphosyntactic, lexical-semantic and pragmatic) and by the skills they are practiced in (reading comprehension, written expression and interaction, listening comprehension and oral expression and interaction) or by exercise type. Ultimately, this analysis allows us to determinate which linguistic features from other varieties are most present in the textbooks, in which communicative language activities they are introduced, as well as which peripheral variety predominates in each textbook.
Low-input CUT&Tag for efficient epigenomic profiling of zebrafish stage I oocytes
Authors: Zheng Qianwen , Wu Xiaotong , Li Xin , Mo Xianming , Xiang Bo , Chen Jing
Published: December 2024

Histone modification signatures mark sites of transcriptional regulatory elements and regions of gene activation and repression. These sites vary among cell types and undergo dynamic changes during development and in diseases. Oocytes produce numerous maternal factors essential for early embryonic development, which are significantly influenced by epigenetic modifications. The profiling of epigenetic modifications during oogenesis remains uniquely challenging due to the presence of numerous tightly wrapped granulosa cells. Here, we successfully established a low-input CUT&Tag (Cleavage Under Targets and Tagmentation) method tailored for zebrafish stage I oocytes. This advanced technique enables high-resolution profiling of histone modifications and DNA-binding proteins, critical for understanding chromatin dynamics in developing oocytes. In this study, we detailed the workflow for this technique, including the isolation of pure stage I oocytes without somatic cells, library construction and quality monitoring. Our results demonstrate the method’s efficacy by identifying distinct histone modification patterns and analyzing differentially expressed genes in oocytes with and without granulosa cells. We also successfully profiled divergent histone modifications in oocytes derived from wild-type and huluwa mutants. These advancements overcome technical challenges in epigenetic research on zebrafish oocytes and establish a solid foundation for exploring the epigenetic regulatory mechanisms of maternal contribution.
Cryptosporidium parvum infection alters the intestinal mucosa transcriptome in neonatal calves: impacts on epithelial barriers and transcellular transport systems
Authors: Veshkini Arash , Kühn Christa , Dengler Franziska , Bachmann Lisa , Liermann Wendy , Helm Christiane , Ulrich Reiner , Delling Cora , Hammon Harald M.
Published: December 2024

Introduction

Cryptosporidium parvum (C. parvum) is the most prevalent enteric protozoan parasite causing infectious diarrhea in neonatal calves worldwide with a direct negative impact on their health and welfare. This study utilized next-generation sequencing (NGS) to deepen our understanding of intestinal epithelial barriers and transport mechanisms in the pathophysiology of infectious diarrhea in neonatal calves, which could potentially unveil novel solutions for treatment.

Methods

At day 1 of life, male Holstein-Friesian calves were either orally infected (n = 5) or not (control group, n = 5) with C. parvum oocysts (in-house strain LE-01-Cp-15). On day 8 after infection, calves were slaughtered and jejunum mucosa samples were taken. The RNA was extracted from collected samples and subjected to sequencing. Differentially expressed genes (DEG) between the infected and CTRL groups were assessed using DESeq2 at a false discovery rate < 0.05 and used for gene ontology (GO) and pathway enrichment analysis in Cytoscape (v3.9.1).

Results and discussion

To study the pathophysiology of infectious diarrhea on intestinal permeability, 459 genes related to epithelial cell barrier integrity and paracellular and transmembrane transport systems were selected from 12,908 identified genes in mucus. Among, there were 61 increased and 109 decreased gene transcripts belonged to adhesion molecules (e.g. ADGRD1 and VCAM1), ATP-binding cassette (ABC, e.g. ABCC2 and ABCD1) and solute carrier (SLC, e.g. SLC28A2 and SLC38A3) transporters, and ion channels (e.g. KCNJ15). Our results suggest deregulation of cellular junctions and thus a possibly increased intestinal permeability, whereas deregulation of ABC and SLC transporters and ion channels may influence the absorption/secretion of amino acids, carbohydrates, fats, and organic compounds, as well as acid-based balance and osmotic hemostasis. Besides pathogen-induced gene expression alterations, part of the DEG may have been triggered or consequently affected by inflammatory mechanisms. The study provided a deeper understanding of the pathophysiology of infectious diarrhea in neonatal calves and the host-pathogen interactions at the transcript level. For further studies with a particular focus on the transport system, these results could lead to a new approach to elucidating pathophysiological regulatory mechanisms.
Analysis of risk factors faced by Chinese international students based on fuzzy comprehensive evaluation model
Authors: Gao Junhong , Zhao Zhiyu , Li Hongwei
Published: December 2024

Introduction

China has contributed the most international students to the global higher-education sector for over a decade. Existing research has indicated various risk factors faced by international students. However, few studies have examined the risks experienced by Chinese international students. Two research questions were investigated in this study: (1) Which risk factor has the greatest impact on Chinese international students when studying abroad? and (2) Which risk factor is most frequently experienced by Chinese international students when studying abroad?

Method

To address these questions, this study proposes a fuzzy comprehensive evaluation model for evaluating the risk factors faced by Chinese international students. The model comprises six second-level factors and 22 third-level indicators, which was empirically examined by data collected through 556 questionnaires. The analytical hierarchy process and factor analysis were employed to calculate the index weight of second-level factors and third-level indicators.

Results

The results reveal that the risk factor, psychological discrepancy, has the greatest impact and is most likely to be experienced by Chinese international students. The paper provides an integrated research perspective to study the risk of international students.

Discussion

The paper discussed the factors that impact the risk faced by Chinese international students. The risk faced by Chinese international students was found to be high. Psychological discrepancy, academic choice, and cross-cultural adaptation were the three risk factors that were most likely to be experienced by Chinese international students. Psychological discrepancy, barriers to study, and unforeseen incidents had the highest impact on total risk. Therefore, attention should be paid to these risk factors to avoid the occurrence of risk and its negative impacts.
Tone superimposition technique in Speech Sciences: a tutorial
Authors: Wang Xin , Jheng Jhe-Yu , McMurray Bob
Published: December 2024

In the literature, we encounter papers reporting manipulating pitch contours in speech tokens for a specific problem to be addressed in experiments (e.g., learning pitch patterns superimposed onto a pseudo-syllable), usually in the field of Speech Perception and Spoken Word Recognition. This type of research often tests listeners’ perceptual and processing skills in tonal languages (e.g., Mandarin, Thai, etc.), and requires superimposing a pitch contour onto a spoken syllable. However, very few studies reported in detail how this critical manipulation was done to meet specific experimental needs. In addition, there was neither specific guideline or description of the techniques being used, nor how ‘natural’ these manipulated tokens sounded in a particular language upon speech synthesis. Because this technique is crucial in establishing the conclusions in various studies, here, we will demonstrate our method of establishing this technique of tone superimposition (i.e., lexical tones in Mandarin) onto English syllables. In line with the open science model, we will also show our stimuli and procedures via OSF for readers to evaluate the validity of this technique. Manipulating the pitch contour in a spoken syllable can be complicated and change the perception of the spoken syllable in a significant way. Thus, we will also show the important factors to be considered in this process for doing research in Speech Sciences.
AI-resistant assessments in higher education: practical insights from faculty training workshops
Authors: Awadallah Alkouk Wejdan , Khlaif Zuheir N.
Published: December 2024

The emergence of generative AI in education introduces both opportunities and challenges, especially in student assessment. This paper explores the transformative influence of generative AI on assessment practices, drawing from recent training workshops conducted with educators in the Global South. It examines how AI can enrich traditional assessment approaches by fostering critical thinking, creativity, and collaboration. The paper introduces innovative frameworks, such as AI-resistant assessments and the Process-Product Assessment Approach, which emphasize evaluating not only the final product but also the student’s interaction with AI tools throughout their learning journey. Additionally, it provides practical strategies for integrating AI into assessments, underscoring the ethical use and preservation of academic integrity. Addressing the complexities of AI adoption, including concerns around academic misconduct, this paper equips educators with tools to navigate the intricacies of human-AI collaboration in learning settings. Finally, it discusses the significance of institutional policies for guiding the ethical use of AI and offers recommendations for faculty development to align with the evolving educational landscape.
Early adolescents’ visions of the future: towards hopeful and sustainable futures?
Authors: Corres Andrea , Cebrián Gisela , Junyent Mercè
Published: December 2024

Education for Sustainable Development (ESD) particularly emphasises developing future-thinking competencies. Nevertheless, this line of thinking still represents a challenge for younger students, who tend to be left behind when deciding and visualising the future within educational spaces. Through a qualitative approach, our research attempts to explore early adolescents’ views concerning probable and preferable futures and how they are related to sustainable development dimensions. For this purpose, we asked 352 students in the fifth and sixth grades of primary schools in the province of Tarragona (Spain) to explain their visions regarding the probable and preferable futures of their city or town. We analysed the data through an inductive-deductive approach. Our findings indicate that the students’ pessimistic visions of the future require a special effort from an ESD viewpoint to educate them towards hopeful futures. Different degrees of complexity in their views of the futures were identified in relation to sustainability, denoting the need to design diagnostic tools that properly guide pedagogical approaches and interventions towards developing sustainability competencies.
Insulin-like growth factor binding protein-6 modulates proliferative antagonism in response to progesterone in breast cancer
Authors: Lariz Francisco J. , Botero Pacha B. , Shoffstall Isabella , Houston Kevin D.
Published: December 2024

Breast cancer is one of the most diagnosed cancers worldwide. The insulin-like growth factor (IGF) system promotes proliferation and survival in breast cancer cells and is regulated by 6 insulin-like growth factor binding proteins (IGFBPs). The IGFBPs sequester IGFs to prolong their half-life and attenuate binding to insulin-like growth factor 1 receptor (IGF1R). While IGFBP-6 has been studied in some cancers it has not been studied extensively in hormone receptor positive breast cancer. Survival analysis using available databases indicated that high IGFBP-6 levels improve overall survival in progesterone receptor positive breast cancers. IGFBP-6 is transcriptionally induced by progesterone in T47D breast cancer cells resulting in increased intracellular and extracellular IGFBP-6 protein. Knockdown of IGFBP-6 resulted in reduced proliferative antagonism when estradiol stimulated T47D cells were cotreated with progesterone and protein levels of both progesterone receptor isoforms (PR-A and PR-B) were decreased following knockdown of IGFBP-6. P21(Cip1/Waf1), which is progesterone responsive, was not induced in response to progesterone following knockdown of IGFBP-6. Cyclin E2, a cell cycle regulator, is induced by progesterone only when IGFBP-6 is knocked down. Stable overexpression of IGFBP-6 in MCF-7 cells resulted in an increase in Epidermal Growth Factor Receptor (EGFR) and this expression was further enhanced when cells were cotreated with progesterone and estradiol. These results indicate that IGFBP-6 is a regulator of progesterone action, and that PR is required for the observed protective effects of IGFBP-6 in breast cancer.
Case report: A novel nonsense mutation in the MARVELD2 gene causes nonsyndromic hearing loss in a China family
Authors: Huang Chuican , Huang Zhenning , Wang Ping , Wu Xijing , Zhou Qiaomiao , Ding Jun , Luo Qing , Wu Weijia , Fan Xialin , Fan Lichun
Published: December 2024

The MARVELD2 gene is located on chromosome 5q13.2 and is associated with autosomal recessive nonsyndromic hearing loss (OMIM: # 610572). In this study, we identified and reported a novel nonsense mutation in MARVELD2 c. 663G > A in a Chinese family. We collected peripheral venous blood from 19 members of the affected family and performed whole exome sequencing to analyze the mutation genotype. A single-nucleotide mutation was detected in MARVELD2. Five individuals in the family carried the MARVELD2 c.663G>A mutation; one of them was homozygous and showed severe congenital deafness and language impairment. The next-generation sequencing results were validated by Sanger sequencing. This study expands the spectrum of MARVELD2 mutations that cause nonsyndromic hearing loss and provides insights into the molecular pathogenesis underlying deafness. This finding has important implications for genetic screening, diagnosis, counseling, and research of deafness-related genes.
Serum IL-23 levels reflect a myeloid inflammatory signature and predict the response to apremilast in patients with psoriatic arthritis
Authors: De Santis Maria , Tonutti Antonio , Isailovic Natasa , Motta Francesca , Rivara Radu Marian , Ragusa Rita , Guidelli Giacomo M. , Caprioli Marta , Ceribelli Angela , Renna Daniela , Luciano Nicoletta , Selmi Carlo
Published: December 2024

Background

The phosphodiesterase 4 (PDE4) inhibitor apremilast downregulates the production of IL-23 and other pro-inflammatory cytokines involved in the pathogenesis of psoriatic arthritis (PsA).

Aim

To investigate the effects of apremilast on the production of cytokines by peripheral blood monocyte-derived macrophages, innate-like lymphocyte cells (ILCs), mucosal-associated invariant T (MAIT) cells, γδ T cells, natural killer (NK) cells, and NKT-like cells from patients with PsA manifesting different clinical responses to the treatment.

Methods

Peripheral blood samples were obtained from patients with PsA at baseline and after 1 and 4 months of apremilast therapy (n = 23) and 20 controls with osteoarthritis. Cytokine expression in peripheral blood monocyte-derived macrophages and ILCs/MAIT/γδT/NK/NKT-like cells was tested by RT-PCR and FACS analyses, respectively; cytokine levels in culture supernatants and sera were analyzed by ELISA.

Results

PsA monocyte-derived macrophages exhibited higher expressions of IL-23, IL-1β, and TNF-α, compared with OA controls, more profoundly in patients responding to apremilast. There were 17/23 (74%) PsA patients who were classified as responders to apremilast at 4 months, and a baseline serum IL-23 >1.4 pg/mL was associated with the responder status (AUCROC 0.79; sensitivity 100%, specificity 68%). Of note, apremilast led to a significantly reduced expression of IL-23 in peripheral blood monocyte-derived macrophages; IL-17 in ILC1 and in T cells of responder patients; IFN-γ in γδ T lymphocytes.

Conclusion

An enhanced myeloid inflammatory signature characterizes PsA monocyte-derived macrophages, and serum IL-23 levels represent candidate biomarkers for PsA response to apremilast.
Developing a digital phenotype to subdivide adult immunosuppressed COVID-19 outcomes within the English Primary Care Sentinel Network
Authors: Leston Meredith , Kar Debasish , Forbes Anna , Jamie Gavin , Wimalaratna Rashmi , Jiwani Gunjan , Ordóñez-Mena José M. , Stewart Daniel E. , Whitaker Heather , Joy Mark , Lee Lennard Y. W. , Hobbs F. D. Richard , de Lusignan Simon
Published: December 2024

Background

Adults classified as immunosuppressed have been disproportionately affected by the COVID-19 pandemic. Compared to the immunocompetent, certain patients are at increased risk of suboptimal vaccine response and adverse health outcomes if infected. However, there has been insufficient work to pinpoint where these risks concentrate within the immunosuppressed spectrum; surveillance efforts typically treat the immunosuppressed as a single entity, leading to wide confidence intervals. A clinically meaningful and computerised medical record (CMR) compatible method to subdivide immunosuppressed COVID-19 data is urgently needed.

Methods

We conducted a rapid scoping review into COVID-19 mortality across UK immunosuppressed categories to assess if differential mortality risk was a viable means of subdivision. We converted the risk hierarchy that surfaced into a pilot digital phenotype—a valueset and series of ontological rules ready to extract immunosuppressed patients from CMR data and stratify outcomes of interest in COVID-19 surveillance dataflows.

Results

The rapid scoping review returned COVID-19 mortality data for all immunosuppressed subgroups assessed and revealed significant heterogeneity across the spectrum. There was a clear distinction between heightened COVID-19 mortality in haematological malignancy and transplant patients and mortality that approached the immunocompetent baseline amongst cancer therapy recipients, autoimmune patients, and those with HIV. This process, complemented by expert clinical input, informed the curation of the five-part digital phenotype now ready for testing in real-world data; its ontological rules will enable mutually exclusive, hierarchical extraction with nuanced time and treatment conditions. Unique categorisations have been introduced, including ‘Bone Marrow Compromised’ and those dedicated to differentiating prescriptions related and unrelated to cancer. Codification was supported by existing reference sets of medical codes; absent or redundant codes had to be resolved manually.

Discussion

Although this work is in its earliest phases, the development process we report has been highly informative. Systematic review, clinical consensus building, and implementation studies will test the validity of our results and address criticisms of the rapid scoping exercise they are predicated on.

Conclusion

Comprehensive testing for COVID-19 has differentiated mortality risks across the immunosuppressed spectrum. This risk hierarchy has been codified into a digital phenotype for differentiated COVID-19 surveillance; this marks a step towards the needs-based management of these patients that is urgently required.