bluuebunny/crossref_metadata_2025 huggingface dataset:
Formaldehyde exposure induces regulatory T cell-mediated immunosuppression via calcineurin-NFAT signalling pathway
Authors: Park Jeongsik , Yang Hyo-Seon , Song Mi-Kyung , Kim Dong Im , Lee Kyuhong
Published: October 2020

In this study, we investigated the effects of Formaldehyde (FA) exposure on splenic immune responses wherein helper T cells become activated and differentiate into effector T and regulatory T cells. BALB/c mice were exposed to two FA concentrations (1.38 mg/m3 and 5.36 mg/m3) for 4 h/day and 5 days/week for 2 weeks. FA-induced immune responses were examined by the production of cytokines, expression of mRNAs, and distributions of helper T cells and regulatory T cells. Moreover, expression of calcineurin and NFATs, regulatory T cell-related signalling proteins, were evaluated. FA exposure suppressed Th2-, Th1-, and Th17-related splenic cytokines in a dose-dependent manner. mRNA expression of splenic cytokines was also decreased by FA exposure, which correlated with decreased cytokine expression. In parallel, FA exposure promoted T cell differentiation into regulatory T cells in a dose-dependent manner supported by the expression of calcineurin and NFAT1. Taken together, our results indicated that FA exposure increases the number of regulatory T cells via calcineurin-NFAT signalling, thereby leading to effector T cell activity suppression with decreased T cell-related cytokine secretion and mRNA expression. These findings provide insight into the mechanisms underlying the adverse effects of FA and accordingly have general implications for human health, particularly in occupational settings.
Cep215 is essential for morphological differentiation of astrocytes
Authors: Kang Donghee , Shin Wonjung , Yoo Hyunjeong , Kim Seongjae , Lee Seongju , Rhee Kunsoo
Published: October 2020

Cep215 (also known as Cdk5rap2) is a centrosome protein which is involved in microtubule organization. Cep215 is also placed at specific subcellular locations and organizes microtubules outside the centrosome. Here, we report that Cep215 is involved in morphological differentiation of astrocytes. Cep215 was specifically localized at the glial processes as well as centrosomes in developing astrocytes. Morphological differentiation of astrocytes was suppressed in the Cep215-deleted P19 cells and in the Cep215-depleted embryonic hippocampal culture. We confirm that the microtubule organizing function of Cep215 is critical for the glial process formation. However, Cep215 is not involved in the regulation of cell proliferation nor cell specification. Based on the results, we propose that Cep215 organizes microtubules for glial process formation during astrocyte differentiation.
An optimized desuccinylase activity assay reveals a difference in desuccinylation activity between proliferative and differentiated cells
Authors: Yuan Taolin , Keijer Jaap , Guo Angela H. , Lombard David B. , de Boer Vincent C. J.
Published: October 2020

Succinylation is a novel post-translational modification identified on many proteins and is involved in multiple biological processes. Succinylation levels are dynamically regulated, balanced by succinylation and desuccinylation processes, and are closely connected to metabolic state in vivo. Sirtuins have been shown to possess NAD+-dependent desuccinylation activity in vitro and in vivo, among which the desuccinylation activity of SIRT5 is most extensively studied. Our understanding of the response of succinylation levels to different metabolic conditions, is hampered by the lack of a fast NAD+-dependent desuccinylation assay in a physiological context. In the present study, we therefore optimized and validated a fluorescence-based assay for measuring NAD+-dependent desuccinylation activity in cell lysates. Our results demonstrated that shorter and stricter reaction time was critical to approach the initial rate of NAD+-dependent desuccinylation activity in crude cell lysate systems, as compared to the desuccinylation reaction of purified His-SIRT5. Analysis of desuccinylation activity in SIRT5 knockout HEK293T cells confirmed the relevance of SIRT5 in cellular desuccinylation activity, as well as the presence of other NAD+-dependent desuccinylase activities. In addition, we were able to analyse desuccinylation and deacetylation activity in multiple cell lines using this assay. We showed a remarkably higher desuccinylase activity, but not deacetylase activity, in proliferative cultured muscle and adipose cells in comparison with their differentiated counterparts. Our results reveal an alteration in NAD+-dependent desuccinylation activity under different metabolic states.
Coordinated self-interference of wave packets: a new route towards classicality for structurally stable systems
Authors: Ćosić M. , Petrović S. , Bellucci S.
Published: October 2020

This is a study of proton transmission through planar channels of tungsten, where a proton beam is treated as an ensemble of noninteracting wave packets. For this system, the structural stability manifests in an appearance of caustic lines, and as an equivalence of self-interference produced waveforms with canonical diffraction patterns. We will show that coordination between particle self-interference is an additional manifestation of the structural stability existing only in ensembles. The main focus of the analysis was on the ability of the coordination to produce classical structures. We have found that the structures produced by the self-interference are organized in a very different manner. The coordination can enhance or suppress the quantum aspects of the dynamics. This behavior is explained by distributions of inflection, undulation, and singular points of the ensemble phase function, and their bifurcations. We have shown that the coordination has a topological origin which allows classical and quantum levels of reality to exist simultaneously. The classical behavior of the ensemble emerges out of the quantum dynamics without a need for reduction of the quantum to the classical laws of motion.
Application of smart solid lipid nanoparticles to enhance the efficacy of 5-fluorouracil in the treatment of colorectal cancer
Authors: Smith Taylor , Affram Kevin , Nottingham Ebony L. , Han Bo , Amissah Felix , Krishnan Sunil , Trevino Jose , Agyare Edward
Published: October 2020

5-Fluorouracil (5-FU) is a standard treatment option for colorectal cancer (CRC) but its rapid metabolism and systemic instability (short half-life) has hindered its therapeutic efficacy. The objective of this study was to develop a novel drug delivery system, solid lipid nanoparticle (SLN), capable of delivering high payload of 5-FU to treat CRC. The rational was to improve 5FU-nanocarrier compatibility and therapeutic efficacy. The SLN-loaded 5-FU was developed by utilizing a Strategic and unique Method to Advance and Refine the Treatment (SMART) of CRC through hot and cold homogenization approach. The SLN was made of unique PEGylated lipids and combination of the surfactants. Cytotoxicity studies, clonogenic assay, flow cytometry and confocal imaging were conducted to evaluate the effectiveness and cellular uptake of 5FU-SLN4 in HCT-116 cancer cells. Pharmacokinetic (PK) parameters and receptor expressions were determined while tumor efficacy studies were conducted on mouse bearing subcutaneous HCT-116 cancer. Among the all the formulations, 5FU-SLN4 was the most effective with particle size of was 263 ± 3 nm, zeta potential was 0.1 ± 0.02 and entrapment efficiency of 81 ± 10%. The IC50 value of 5FU-SLN4 (7.4 ± 0.02 µM) was 2.3 fold low compared with 5-FU (17.7 ± 0.03 µM). For tumor efficacy studies, 5FU-SLN4 significantly inhibited tumor growth in comparison to 5-FU while area-under plasma concentration-time curve (AUC) of 5FU-SLN4 was 3.6 fold high compared with 5-FU. HER2 receptors expression were markedly reduced in 5-FU-SLN4 treated mice compared with 5FU and liver and kidney tissues showed no toxicity at dose of 20 mg/kg. 5FU-SLN4 was highly cytotoxic against HCT-116 cells and significantly inhibited subcutaneous tumor growth in mice compared with 5-FU. This emphasizes the significance of developing a smart nano-delivery system to optimize the delivery efficiency of anticancer drugs to tumors.
Cellular senescence-mediated exacerbation of Duchenne muscular dystrophy
Authors: Sugihara Hidetoshi , Teramoto Naomi , Nakamura Katsuyuki , Shiga Takanori , Shirakawa Taku , Matsuo Masafumi , Ogasawara Masashi , Nishino Ichizo , Matsuwaki Takashi , Nishihara Masugi , Yamanouchi Keitaro
Published: October 2020

Duchenne muscular dystrophy (DMD) is a progressive disease characterised by chronic muscle degeneration and inflammation. Our previously established DMD model rats (DMD rats) have a more severe disease phenotype than the broadly used mouse model. We aimed to investigate the role of senescence in DMD using DMD rats and patients. Senescence was induced in satellite cells and mesenchymal progenitor cells, owing to the increased expression of CDKN2A, p16- and p19-encoding gene. Genetic ablation of p16 in DMD rats dramatically restored body weight and muscle strength. Histological analysis showed a reduction of fibrotic and adipose tissues invading skeletal muscle, with increased muscle regeneration. Senolytic drug ABT263 prevented loss of body weight and muscle strength, and increased muscle regeneration in rats even at 8 months—the late stage of DMD. Moreover, senescence markers were highly expressed in the skeletal muscle of DMD patients. In situ hybridization of CDKN2A confirmed the expression of it in satellite cells and mesenchymal progenitor cells in patients with DMD. Collectively, these data provide new insights into the integral role of senescence in DMD progression.
Host-symbiont coevolution, cryptic structure, and bleaching susceptibility, in a coral species complex (Scleractinia; Poritidae)
Authors: Forsman Z. H. , Ritson-Williams R. , Tisthammer K.H. , Knapp I. S. S. , Toonen R. J.
Published: October 2020

The ‘species’ is a key concept for conservation and evolutionary biology, yet the lines between population and species-level variation are often blurred, especially for corals. The ‘Porites lobata species complex’ consists of branching and mounding corals that form reefs across the Pacific. We used reduced representation meta-genomic sequencing to examine genetic relationships within this species complex and to identify candidate loci associated with colony morphology, cryptic genetic structure, and apparent bleaching susceptibility. We compared existing Porites data with bleached and unbleached colonies of the branching coral P. compressa collected in Kāneʻohe Bay Hawaiʻi during the 2015 coral bleaching event. Loci that mapped to coral, symbiont, and microbial references revealed genetic structure consistent with recent host-symbiont co-evolution. Cryptic genetic clades were resolved that previous work has associated with distance from shore, but no genetic structure was associated with bleaching. We identified many candidate loci associated with morphospecies, including candidate host and symbiont loci with fixed differences between branching and mounding corals. We also found many loci associated with cryptic genetic structure, yet relatively few loci associated with bleaching. Recent host-symbiont co-evolution and rapid diversification suggests that variation and therefore the capacity of these corals to adapt may be underappreciated.
Pollen tube growth from multiple pollinator visits more accurately quantifies pollinator performance and plant reproduction
Authors: Stavert Jamie R. , Bailey Charlie , Kirkland Lindsey , Rader Romina
Published: October 2020

Pollination services from animals are critical for both crop production and reproduction in wild plant species. Accurately measuring the relative contributions of different animal taxa to pollination service delivery is essential for identifying key pollinators. However, widely used measures of pollinator effectiveness (e.g., single visit pollen deposition) may be inaccurate where plant reproduction is strongly constrained by pollen quality. Here, we test the efficacy of single and multiple pollinator visits for measuring pollinator performance in a model plant species (apple,Malus domesticaBorkh) that is strongly limited by pollen quality. We determined pollination success using a suite of measures (pollen deposition, pollen tube growth, fruit and seed set) from single and multiple pollinator visits. We found that pollen deposition from a single pollinator visit seldom resulted in the growth of pollen tubes capable of eliciting ovule fertilisation and never resulted in fruit or seed production. In contrast, multiple pollinator visits frequently initiated the growth of pollen tubes capable of ovule fertilisation and often led to fruit and seed production. Our findings suggest that single visit pollen deposition may provide a poor measure of pollinator performance when linked to reproductive success of plant species that are constrain by pollen quality. Alternatively, pollen tube growth from single and multiple pollinator visits can provide a measure of pollinator performance that is more closely linked to plant reproduction.
Valley-selective energy transfer between quantum dots in atomically thin semiconductors
Authors: Baimuratov Anvar S. , Högele Alexander
Published: October 2020

In monolayers of transition metal dichalcogenides the nonlocal nature of the effective dielectric screening leads to large binding energies of excitons. Additional lateral confinement gives rise to exciton localization in quantum dots. By assuming parabolic confinement for both the electron and the hole, we derive model wave functions for the relative and the center-of-mass motions of electron–hole pairs, and investigate theoretically resonant energy transfer among excitons localized in two neighboring quantum dots. We quantify the probability of energy transfer for a direct-gap transition by assuming that the interaction between two quantum dots is described by a Coulomb potential, which allows us to include all relevant multipole terms of the interaction. We demonstrate the structural control of the valley-selective energy transfer between quantum dots.
Selective effects of protein 4.1N deficiency on neuroendocrine and reproductive systems
Authors: Wang Hua , Parra Marilyn , Conboy John G. , Hillyer Christopher D. , Mohandas Narla , An Xiuli
Published: October 2020

Protein 4.1N, a member of the protein 4.1 family, is highly expressed in the brain. But its function remains to be fully defined. Using 4.1N−/− mice, we explored the function of 4.1N in vivo. We show that 4.1N−/− mice were born at a significantly reduced Mendelian ratio and exhibited high mortality between 3 to 5 weeks of age. Live 4.1N−/− mice were smaller than 4.1N+/+ mice. Notably, while there were no significant differences in organ/body weight ratio for most of the organs, the testis/body and ovary/body ratio were dramatically decreased in 4.1N−/− mice, demonstrating selective effects of 4.1N deficiency on the development of the reproductive systems. Histopathology of the reproductive organs showed atrophy of both testis and ovary. Specifically, in the testis there is a lack of spermatogenesis, lack of leydig cells and lack of mature sperm. Similarly, in the ovary there is a lack of follicular development and lack of corpora lutea formation, as well as lack of secretory changes in the endometrium. Examination of pituitary glands revealed that the secretory granules were significantly decreased in pituitary glands of 4.1N−/− compared to 4.1N+/+. Moreover, while GnRH was expressed in both neuronal cell body and axons in the hypothalamus of 4.1N+/+ mice, it was only expressed in the cell body but not the axons of 4.1N-/- mice. Our findings uncover a novel role for 4.1N in the axis of hypothalamus-pituitary gland-reproductive system.
Synbiotic feed supplementation significantly improves lipid utilization and shows discrete effects on disease resistance in rainbow trout (Oncorhynchus mykiss)
Authors: Villumsen Kasper Rømer , Ohtani Maki , Forberg Torunn , Aasum Elisabeth , Tinsley John , Bojesen Anders Miki
Published: October 2020

Enteric redmouth disease caused by the bacterial pathogen Yersinia ruckeri is the main reason for antimicrobial prescription, and a cause of substantial economic losses and decreased animal welfare in aquaculture. Given the importance of the intestinal microbiota in digestion and disease, our aim was to investigate whether synbiotic feed supplementation strategies could improve feed performance and disease resistance. Four experimental synbiotic feeds formulated with pre- and probiotics were tested against a commercially available probiotic control feed. Each experimental feed was evaluated for feed performance, effects on gross as well as intestinal morphometrics, and finally their effect on resistance against a waterborne experimental infection with Yersinia ruckeri serotype O1, biotype 2. While co-supplementing Pediococcus acidilactici with citrus flavonoids or bacterial paraprobiotics significantly improved utilization of feed lipid content relative to the control group, a decrease in lipid utilization was observed for feeds that combined P. acidilactici with yeast paraprobiotics. No significant improvements on disease resistance were observed. Still, synbiotic formulations including P. acidilactici led to reduced risks relative to that of the control group, while an increased relative risk was observed for a Bacillus-based formulation. In conclusion, two of the synbiotic supplements significantly improved lipid utilization and contributed to minor increases in disease resistance.
Optimal collective decision making: consensus, accuracy and the effects of limited access to information
Authors: Berekméri Evelin , Zafeiris Anna
Published: October 2020

“Knowledge is power”—holds the popular proverb, because knowledge and information is indeed one of the cornerstones of effective decision making, a requisite all living beings face continually. In fact, effective decision making is a matter of life and death, for individuals and groups alike. Furthermore, in case of group decisions, consensus is also often desirable. This latter one has been studied extensively by means of formal (mathematical) tools (in the field of opinion dynamics), while the first requirement, the process of yielding accurate information has been largely neglected, at least so far. In the present paper we study the optimal structure of groups which are embedded into an external, observable environment for (i) reaching consensus (ii) having well-informed members, and (iii) for those cases when both aspects are equally important. The groups are characterised by their communication networks and individual properties. We find that the group structures fundamentally differ from each other since having well-informed members requires highly specialised individuals embedded into a structured communication network, while consensus is promoted by non-hierarchical networks in which individuals participate equally. We also find that—contrary to intuition—high access to information calls forth hierarchy, and that suggestibility promotes accuracy, not consensus.
A tri-component knee plug for the 3rd generation of autologous chondrocyte implantation
Authors: Tayebi Lobat , Cui Zhanfeng , Ye Hua
Published: October 2020

Here, we report a newly designed knee plug to be used in the 3rd generation of Autologous Chondrocyte Implantation (ACI) in order to heal the damaged knee cartilage. It is composed of three components: The first component (Bone Portion) is a 3D printed hard scaffold with large pores (~ 850 µm), made by hydroxyapatite and β-tricalcium phosphate to accommodate the bony parts underneath the knee cartilage. It is a cylinder with a diameter of 20 mm and height of 7.5 mm, with a slight dome shape on top. The plug also comprises a Cartilage Portion (component 2) which is a 3D printed gelatin/elastin/sodium-hyaluronate soft thick porous membrane with large pores to accommodate chondrocytes. Cartilage Portion is secured on top of the Bone Portion using mechanical interlocking by designing specific knobs in the 3D printed construct of the Cartilage Portion. The third component of the plug (Film) is a stitchable permeable membrane consisting of polycaprolactone (PCL) on top of the Cartilage Portion to facilitate sliding of the knee joint and to hold the entire plug in place while allowing nutrients delivery to the Cartilage Portion. The PCL Film is prepared using a combination of film casting and sacrificial material leaching with a pore size of 10 µm. It is surface modified to have specific affinity with the Cartilage Portion. The detailed design criteria and production process of this plug is presented in this report. Full in vitro analyses have been performed, which indicate the compatibility of the different components of the plug relative to their expected functions.
Unsupervised learning for large-scale corneal topography clustering
Authors: Zéboulon Pierre , Debellemanière Guillaume , Gatinel Damien
Published: October 2020

Machine learning algorithms have recently shown their precision and potential in many different use cases and fields of medicine. Most of the algorithms used are supervised and need a large quantity of labeled data to achieve high accuracy. Also, most applications of machine learning in medicine are attempts to mimic or exceed human diagnostic capabilities but little work has been done to show the power of these algorithms to help collect and pre-process a large amount of data. In this study we show how unsupervised learning can extract and sort usable data from large unlabeled datasets with minimal human intervention. Our digital examination tools used in clinical practice store such databases and are largely under-exploited. We applied unsupervised algorithms to corneal topography examinations which remains the gold standard test for diagnosis and follow-up of many corneal diseases and refractive surgery screening. We could extract 7019 usable examinations which were automatically sorted in 3 common diagnoses (Normal, Keratoconus and History of Refractive Surgery) from an unlabeled database with an overall accuracy of 96.5%. Similar methods could be used on any form of digital examination database and greatly speed up the data collection process and yield to the elaboration of stronger supervised models.
Reduction in hsCRP levels is associated with decreased incidence of cardiovascular events in Japanese hypertensive women but not in men
Authors: Kim-Mitsuyama Shokei , Soejima Hirofumi , Yasuda Osamu , Node Koichi , Jinnouchi Hideaki , Yamamoto Eiichiro , Sekigami Taiji , Ogawa Hisao , Matsui Kunihiko
Published: October 2020

To test our hypothesis that the magnitude of reduction in hsCRP achieved by antihypertensive medications may predict the benefit for cardiovascular outcomes in hypertensive individuals, we performed subanalysis of the ATTEMPT-CVD study. The hypertensive participants enrolled in the ATTMEPT-CVD study were categorized into two groups according to whether achieved reduction in hsCRP levels at 6 months after initiation of antihypertensive medications from baseline was equal to or greater than 40% (responder group) or less than 40% (non-responder group). Baseline characteristics and blood pressure during follow-up period were similar between the groups. For women, the incidence of cardiovascular events was significantly less in responder group than non-responder group (P < 0.0221). However, for men, there was no significant difference between the groups regarding incident cardiovascular events (P = 0.2434). There was a significant interaction (P = 0.0187) between sexes for incident cardiovascular events. Our results provide the evidence suggesting that substantial reduction (40% or greater reduction) in hsCRP on antihypertensive medication predicts the benefit for cardiovascular outcomes in hypertensive women but it does not in hypertensive men. The magnitude of achieved reduction in hsCRP by antihypertensive medications seems to be a useful indicator of successful treatment in Japanese hypertensive women.

This trial was registered with ClinicalTrials.gov, number NCT01075698.
Size-specific recolonization success by coral-dwelling damselfishes moderates resilience to habitat loss
Authors: Pratchett Morgan S. , Messmer Vanessa , Wilson Shaun K.
Published: October 2020

Increasing degradation of coral reef ecosystems and specifically, loss of corals is causing significant and widespread declines in the abundance of coral reef fishes, but the proximate cause(s) of these declines are largely unknown. Here, we examine specific responses to host coral mortality for three species of coral-dwelling damselfishes (Dascyllus aruanus, D. reticulatus, and Pomacentrus moluccensis), explicitly testing whether these fishes can successfully move and recolonize nearby coral hosts. Responses of fishes to localized coral loss was studied during population irruptions of coral feeding crown-of-thorns starfish, where starfish consumed 29 (34%) out of 85 coral colonies, of which 25 (86%) were occupied by coral-dwelling damselfishes. Damselfishes were not tagged or individually recognizable, but changes in the colonization of different coral hosts was assessed by carefully assessing the number and size of fishes on every available coral colony. Most damselfishes (> 90%) vacated dead coral hosts within 5 days, and either disappeared entirely (presumed dead) or relocated to nearby coral hosts. Displaced fishes only ever colonized corals already occupied by other coral-dwelling damselfishes (mostly conspecifics) and colonization success was strongly size-dependent. Despite movement of damselfishes to surviving corals, the local abundance of coral-dependent damselfishes declined in approximate accordance with the proportional loss of coral habitat. These results suggest that even if alternative coral hosts are locally abundant, there are significant biological constraints on movement of coral-dwelling damselfishes and recolonization of alternative coral habitats, such that localized persistence of habitat patches during moderate or patchy disturbances do not necessarily provide resilience against overall habitat loss.
Reduced resting state functional connectivity with increasing age-related hearing loss and McGurk susceptibility
Authors: Schulte Alina , Thiel Christiane M. , Gieseler Anja , Tahden Maike , Colonius Hans , Rosemann Stephanie
Published: October 2020

Age-related hearing loss has been related to a compensatory increase in audio-visual integration and neural reorganization including alterations in functional resting state connectivity. How these two changes are linked in elderly listeners is unclear. The current study explored modulatory effects of hearing thresholds and audio-visual integration on resting state functional connectivity. We analysed a large set of resting state data of 65 elderly participants with a widely varying degree of untreated hearing loss. Audio-visual integration, as gauged with the McGurk effect, increased with progressing hearing thresholds. On the neural level, McGurk illusions were negatively related to functional coupling between motor and auditory regions. Similarly, connectivity of the dorsal attention network to sensorimotor and primary motor cortices was reduced with increasing hearing loss. The same effect was obtained for connectivity between the salience network and visual cortex. Our findings suggest that with progressing untreated age-related hearing loss, functional coupling at rest declines, affecting connectivity of brain networks and areas associated with attentional, visual, sensorimotor and motor processes. Especially connectivity reductions between auditory and motor areas were related to stronger audio-visual integration found with increasing hearing loss.
Sea ice variability and maritime activity around Svalbard in the period 2012–2019
Authors: Stocker Alexandra N. , Renner Angelika H. H. , Knol-Kauffman Maaike
Published: October 2020

Climate change is strongly impacting the Arctic environment, leading to rapid sea ice loss. In some sectors, the retreating ice edge is perceived as an opportunity to expand and develop economic activities. Previous studies show this development in the Canadian and Russian Arctic. This paper examines mobility patterns of cruise ships and fishing vessels around Svalbard, a major hotspot of maritime activity and retreating sea ice cover, in relation to sea ice variability between August 2012 and September 2019. The results show a slight overall increase in fisheries and cruise activity, as well as remarkable trends of stretching operational seasons and expanding navigational areas in these sectors. Overall increasing activity and changing mobility patterns provoke a discussion about the implications for safe navigation and sustainable management, thus raising issues of high pan-Arctic relevance.
Effects of exogenous α-oxoglutarate on proline accumulation, ammonium assimilation and photosynthesis of soybean seedling (Glycine max(L.) Merr.) exposed to cold stress
Authors: Gai Zhijia , Liu Lei , Zhang Jingtao , Liu Jingqi , Cai Lijun
Published: October 2020

The objective of this study was to examine the effects of exogenous α-oxoglutarate on leaf proline accumulation, ammonium assimilation and photosynthesis of soybean when exposed to cold stress. To achieve this objective, exogenous α-oxoglutarate was sprayed to potted seedlings of Henong60 and Heinong48 at 0, 2.5, 5.0 and 7.5 mmol/L, identified as A0, A2.5, A5.0, and A7.5, respectively. Leaf samples were collected after cold stress of 24 h (S1 stage) and 48 h (S2 stage). The results indicated that exogenous α-oxoglutarate significantly enhanced leaf GS activity, NADP-GDH activity, glutamate content, proline content and photosynthesis of soybean seedling exposed to cold stress at S1 and S2 stages. The ammonium content in leaf was significantly decreased by exogenous α-oxoglutarate at both stages. 5.0 mmol/L of exogenous α-oxoglutarate is the optimum concentration in this study. Leaf proline content for Henong60 and Heinong48 at A5.0 was 37.53% and 17.96% higher than that at A0 at S1 stage, respectively. Proline content for Henong60 and Heinong48 increased by 28.82% and 12.41% at A5.0 and A0, respectively, at S2 stage. Those results suggested that exogenous α-oxoglutarate could alleviate the adverse effects of cold stress.
Early-pregnancy transcriptome signatures of preeclampsia: from peripheral blood to placenta
Authors: Yadama Aishwarya P. , Maiorino Enrico , Carey Vincent J. , McElrath Thomas F. , Litonjua Augusto A. , Loscalzo Joseph , Weiss Scott T. , Mirzakhani Hooman
Published: October 2020

Several studies have linked maternal asthma, excess BMI, and low vitamin D status with increased risk of Preeclampsia (PE) development. Given prior evidence in the literature and our observations from the subjects in the Vitamin D Antenatal Asthma Reduction Trial (VDAART), we hypothesized that PE, maternal asthma, vitamin D insufficiency, and excess body mass index (BMI) might share both peripheral blood and placental gene signatures that link these conditions together. We used samples collected in the VDAART to investigate relationships between these four conditions and gene expression patterns in peripheral blood obtained at early pregnancy. We identified a core set of differentially expressed genes in all comparisons between women with and without these four conditions and confirmed them in two separate sets of samples. We confirmed the differential expression of the shared gene signatures in the placenta from an independent study of preeclampsia cases and controls and constructed the preeclampsia module using protein–protein interaction networks. CXC chemokine genes showed the highest degrees of connectivity and betweenness centrality in the peripheral blood and placental modules. The shared gene signatures demonstrate the biological pathways involved in preeclampsia at the pre-clinical stage and may be used for the prediction of preeclampsia.
Antimicrobial resistant and enteropathogenic bacteria in ‘filth flies’: a cross-sectional study from Nigeria
Authors: Onwugamba Francis Chinedu , Mellmann Alexander , Nwaugo Victor Oluoha , Süselbeck Benno , Schaumburg Frieder
Published: October 2020

‘Filth flies’ facilitate the dispersal of pathogens between animals and humans. The objective was to study the intestinal colonization with antimicrobial resistant and enteropathogenic bacteria in ‘filth flies’ from Nigeria. Flies from Southern Nigeria were screened for extended-spectrum β-lactamase producing Enterobacterales (ESBL-E), Staphylococcus aureus, Salmonella sp., Shigella sp., Campylobacter sp. and Yersinia enterocolitica by culture. ESBL-E were tested for blaSHV, blaCTX-M and blaTEM; S. aureus was screened for enterotoxins. Spa typing and multilocus sequence typing (MLST) was done for S. aureus and MLST for Escherichia coli. Of 2,000 flies, 400 were randomly collected for species identification. The most common species were Musca domestica (44.8%, 179/400), Chrysomya putoria (21.6%, 85/400) and Musca sorbens (18.8%, 75/400). Flies were colonized with S. aureus (13.8%, 275/2,000) and ESBL-E (0.8%, 16/2,000). No other enteropathogenic bacteria were detected. The enterotoxin sei was most common (26%, 70/275) in S. aureus, followed by sea (12%, n = 32/275). Four S. aureus isolates were methicillin resistant (mecA positive, t674 and t5305, ST15). The blaCTX-M (n = 16) was the most prevalent ESBL subtype, followed by blaTEM (n = 8). ‘Filth flies’ can carry antimicrobial resistant bacteria in Nigeria. Enterotoxin-positive S. aureus might be the main reason for food poisoning by ‘filth flies’ in the study area.
Experimental and mechanistic research on modifying the mechanic properties of the high water backfill material by electrochemical treatment
Authors: Xie Shengrong , Sun Yaohui , Wang En , Chen Dongdong , Wu Xiaoyu , Qi Pengyu
Published: October 2020

To promote the engineering applications of high water backfill materials (HWBM) in mining, a series of experiments are performed to investigate the effects of the direct current (DC) electric field on the mechanic properties and electrical resistivity of HWBMs. Based on X-ray diffraction (XRD) and scanning electron microscopy (SEM) investigations, the influence of electrochemical treatment on the hydration products and the microstructure of the HWBM was studied. The results show that the peak strength, elastic modulus, deformation modulus and electrical resistivity of the HWBM samples all first increased and then decreased with the increasing of the potential gradient, and the peak points appeared when the potential gradient was 0.2 V/cm. The anisotropy of content of ettringite and calcium silicate hydrates (C–S–H) increased betweent the anodic and cathodic regions of samples. Meanwhile, microstructure in the anodic region of the samples was more stable after electrochemical treatment, which indicates that the different variation of mineralogical compositions and microstructures in different regions of the samples are the primary factors affecting the mechanic properties and electrical resistivity of the HWBM. Therefore, the electrochemical method is a potential technology to modify the engineering properties of the HWBM.
Increased prevalence of depression in South Korea from 2002 to 2013
Authors: Kim Ga Eun , Jo Min-Woo , Shin Yong-Wook
Published: October 2020

South Korea has one of the highest suicide rates among countries. However, the prevalence of depression in South Korea has been reported to be much lower than in other countries. The current study aims to estimate the prevalence of major depressive disorder using a large representative sample of the South Korean population. The prevalence of depression in a sampled population of one million individuals increased from 2.8% in 2002 to 5.3% in 2013; it was found to increase with the age of the population, and was higher in females than in males for most age groups. A Cox’s proportional hazard model showed that suicide risk was significantly higher in people with depression (hazard ratio [HR] 3.79, 95% CI 3.14–4.58) than those without depression. It was also significantly higher in older people (HR 1.52, 95% CI 1.36–1.70) than in younger people, and in males (HR 2.45, 95% CI 2.02–2.96) than in females. Furthermore, higher income groups were at lower suicide risk as compared to lower income groups (HR 0.88, 95% CI 0.80–0.95). This study using the large representative sample data provided evidence that increased prevalence of depression contributed to the increased risk of suicide in South Korea during the recent decade.
Mating system and population structure in the natural distribution of Toona ciliata (Meliaceae) in South China
Authors: Zhou Wei , Zhang Xin-Xin , Ren Ying , Li Pei , Chen Xiao-Yang , Hu Xin-Sheng
Published: October 2020

Most initially perfect flowers of Toona ciliata Roem subsequently develop into functionally unisexual flowers and their relative positions in the same inflorescence could enhance the outcrossing system in this species. Here we investigated the mating system of this species. We used eight nuclear microsatellite markers and investigated the progeny of 125 mother trees from six populations naturally distributed in South China, with sample sizes ranging from 64 to 300 seeds. The multilocus outcrossing rate was 0.970 ± 0.063, and the single locus outcrossing rate was 0.859 ± 0.106, indicating the pattern of predominant outcrossing. Selfing was present in one population, but biparental inbreeding occurred in five populations. Inbreeding was absent in maternal parents, and correlations of selfing among families or among loci were generally insignificant. Positive correlation of paternity at multiple loci was significant in four populations, but was not consistent with the results at single loci. Population substructure occurred in male similarity between outcrosses only in one population. Population genetic differentaitaion was significant (Fst = 34.5%) and the effects of isolation-by-distance at the eight loci were significant among the six populations. These results provide evidence that self-comptability and inbreeding naturally occur in T. ciliata and indicate that inbreeding avoidance is necessary during genetic improvement and breeding of this endangered tree species.
Incorporating a monetary variable into the Schelling model addresses the issue of a decreasing entropy trace
Authors: Mantzaris Alexander V.
Published: October 2020

The Schelling model of segregation has been shown to have a simulation trace which decreases the entropy of its states as the aggregate number of residential agents surrounded by a threshold of equally labeled agents increases. This introduces a paradox which goes against the second law of thermodynamics that states how entropy must increase. In the efforts to bring principles of physics into the modeling of sociological phenomena this must be addressed. A modification of the model is introduced where a monetary variable is provided to the residential agents (sampled from reported income data), and a dynamic which acts upon this variable when an agent changes its location on the grid. The entropy of the simulation over the iterations is estimated in terms of the aggregate residential homogeneity and the aggregate income homogeneity. The dynamic on the monetary variable shows that it can increase the entropy of the states over the simulation. The path of the traces with both variables in the results show that the shape of the region of entropy is followed supporting that the decrease of entropy due to the residential clustering has a parallel and independent effect increasing the entropy via the monetary variable.
Sleep disordered breathing and subjective excessive daytime sleepiness in relation to the risk of motor vehicle crash: the Toon Health Study
Authors: Matsuo Ryotaro , Tanigawa Takeshi , Tomooka Kiyohide , Ikeda Ai , Wada Hiroo , Maruyama Koutatsu , Saito Isao
Published: October 2020

Sleep disordered breathing (SDB) is a significant cause of motor vehicle crash (MVC). We conducted a prospective cohort study among 1047 Japanese community-dwellers to detect whether the presence or absence of subjective excessive daytime sleepiness (EDS) affect the association of SDB with a risk of MVC. SDB was assessed by a single airflow monitor which measured the respiratory disturbance index (RDI) during one-night, and participants were classified into the SDB group (RDI ≥ 10) and non-SDB group (RDI < 10). Subjective EDS was defined as Japanese version of Epworth Sleepiness Scale scores ≥ 11. A follow-up questionnaire five years after the baseline ascertained history of MVC over the period. Multivariable logistic regression analysis examined the association between SDB and MVC after stratification by subjective EDS. The multivariable-adjusted odds ratios (95% confidence interval) for MVC among the female SDB group were 1.66 (1.05–2.63) compared with the non-SDB group, and this association was more evident in females without subjective EDS [1.84(1.02–3.32)], but not among those with subjective EDS. There was no significant association in males. These findings indicate that SDB screening should be recommended regardless of subjective EDS to prevent SDB-related MVC among general population, particularly in females.
Deep neural network based artificial intelligence assisted diagnosis of bone scintigraphy for cancer bone metastasis
Authors: Zhao Zhen , Pi Yong , Jiang Lisha , Xiang Yongzhao , Wei Jianan , Yang Pei , Zhang Wenjie , Zhong Xiao , Zhou Ke , Li Yuhao , Li Lin , Yi Zhang , Cai Huawei
Published: October 2020

Bone scintigraphy (BS) is one of the most frequently utilized diagnostic techniques in detecting cancer bone metastasis, and it occupies an enormous workload for nuclear medicine physicians. So, we aimed to architecture an automatic image interpreting system to assist physicians for diagnosis. We developed an artificial intelligence (AI) model based on a deep neural network with 12,222 cases of 99mTc-MDP bone scintigraphy and evaluated its diagnostic performance of bone metastasis. This AI model demonstrated considerable diagnostic performance, the areas under the curve (AUC) of receiver operating characteristic (ROC) was 0.988 for breast cancer, 0.955 for prostate cancer, 0.957 for lung cancer, and 0.971 for other cancers. Applying this AI model to a new dataset of 400 BS cases, it represented comparable performance to that of human physicians individually classifying bone metastasis. Further AI-consulted interpretation also improved human diagnostic sensitivity and accuracy. In total, this AI model performed a valuable benefit for nuclear medicine physicians in timely and accurate evaluation of cancer bone metastasis.
A scoping review of market links between value chain actors and small-scale producers in developing regions
Authors: Liverpool-Tasie Lenis Saweda O. , Wineman Ayala , Young Sarah , Tambo Justice , Vargas Carolina , Reardon Thomas , Adjognon Guigonan Serge , Porciello Jaron , Gathoni Nasra , Bizikova Livia , Galiè Alessandra , Celestin Ashley
Published: October 2020

Sustainable Development Goal 2 aims to end hunger, achieve food and nutrition security and promote sustainable agriculture by 2030. This requires that small-scale producers be included in, and benefit from, the rapid growth and transformation under way in food systems. Small-scale producers interact with various actors when they link with markets, including product traders, logistics firms, processors and retailers. The literature has explored primarily how large firms interact with farmers through formal contracts and resource provision arrangements. Although important, contracts constitute a very small share of smallholder market interactions. There has been little exploration of whether non-contract interactions between small farmers and both small- and large-scale value chain actors have affected small farmers’ livelihoods. This scoping review covers 202 studies on that topic. We find that non-contract interactions, de facto mostly with small and medium enterprises, benefit small-scale producers via similar mechanisms that the literature has previously credited to large firms. Small and medium enterprises, not just large enterprises, address idiosyncratic market failures and asset shortfalls of small-scale producers by providing them, through informal arrangements, with complementary services such as input provision, credit, information and logistics. Providing these services directly supports Sustainable Development Goal 2 by improving farmer welfare through technology adoption and greater productivity.
A scoping review of the contributions of farmers’ organizations to smallholder agriculture
Authors: Bizikova Livia , Nkonya Ephraim , Minah Margitta , Hanisch Markus , Turaga Rama Mohana Rao , Speranza Chinwe Ifejika , Karthikeyan Muthumariappan , Tang Lixia , Ghezzi-Kopel Kate , Kelly Julie , Celestin Ashley Casandra , Timmers Beth
Published: October 2020

Farmers’ organizations (FOs), such as associations, cooperatives, self-help and women’s groups, are common in developing countries and provide services that are widely viewed as contributing to income and productivity for small-scale producers. Here, we conducted a scoping review of the literature on FO services and their impacts on small-scale producers in sub-Saharan Africa and India. Most reviewed studies (57%) reported positive FO impacts on farmer income, but much fewer reported positive impacts on crop yield (19%) and production quality (20%). Environmental benefits, such as resilience-building and improved water quality and quantity were documented in 24% of the studies. Our analysis indicates that having access to markets through information, infrastructure, and logistical support at the centre of FO design could help integrate FOs into policy. Natural resource management should also be more widely incorporated in the services provided by FOs to mitigate risks associated with environmental degradation and climate change. Finally, farmers who are already marginalized because of poor education, land access, social status and market accessibility may require additional support systems to improve their capacities, skills and resources before they are able to benefit from FO membership.
Quantification of schizophyllan directly from the fermented broth by ATR-FTIR and PLS regression
Authors: Mansoldo Felipe Raposo Passos , Cardoso Veronica da Silva , Neves Junior Athayde , Cedrola Sabrina Martins Lage , Maricato Victor , Rosa Maria do Socorro S. , Vermelho Alane Beatriz
Published: October 2020

Non-destructive methods that allow the quantification of bioproducts in a simple and quick manner during fermentation are extremely desirable from a practical point of view.
Engineering a macroporous fibrin-based sequential interpenetrating polymer network for dermal tissue engineering
Authors: Gsib Olfat , Eggermont Loek J. , Egles Christophe , Bencherif Sidi A.
Published: October 2020

Macroporous and mechanically reinforced sequential IPN hydrogels combine the biological activity of fibrin with the robust mechanical properties of PEG to generate advanced scaffolds for dermal tissue engineering.
Accurate elemental boiling points from first principles
Authors: Mewes Jan-Michael , Smits Odile R.
Published: October 2020

Thermodynamic integration with a DFT Hamiltonian has been used to obtain accurate absolute Gibbs energies of atomic liquids and normal boiling points in excellent agreement with experimental references (MAD < 2%).
Fabrication of a CuZn-based catalyst using a polyethylene glycol surfactant and supercritical drying
Authors: Zhang Peipei , Peng Xiaobo , Araki Yuya , Fang Yuan , Zeng Yan , Kosol Rungtiwa , Yang Guohui , Tsubaki Noritatsu
Published: October 2020

We employed PEG treatment and supercritical CO2 drying to improve the traditional co-precipitation method for fabrication of CuZn-based catalysts for alcohol-assisted low-temperature methanol synthesis.
Enhanced electrochemical oxygen evolution reaction activity on natural single-atom catalysts transition metal phthalocyanines: the substrate effect
Authors: Wan Xuhao , Niu Huan , Yin Yiheng , Wang Xiting , Shao Chen , Zhang Zhaofu , Guo Yuzheng
Published: October 2020

Some TMPc are excellent OER electrocatalysts and photocatalysts with a single-wall nanotube (SWNT) substrate which can enhance their catalytic activity.