bluuebunny/crossref_metadata_2025 huggingface dataset:
Habitat Quality Differentiation and Consequences for Ecosystem Service Provision of an Amazonian Hyperdominant Tree Species
Authors: Thomas Evert , Jansen Merel , Chiriboga-Arroyo Fidel , Wadt Lúcia H. O. , Corvera-Gomringer Ronald , Atkinson Rachel Judith , Bonser Stephen P. , Velasquez-Ramirez Manuel Gabriel , Ladd Brenton
Published: March 2021

Ecosystem services of Amazonian forests are disproportionally produced by a limited set of hyperdominant tree species. Yet the spatial variation in the delivery of ecosystem services by individual hyperdominant species across their distribution ranges and corresponding environmental gradients is poorly understood. Here, we use the concept of habitat quality to unravel the effect of environmental gradients on seed production and aboveground biomass (AGB) of the Brazil nut, one of Amazonia’s largest and most long-lived hyperdominants. We find that a range of climate and soil gradients create trade-offs between density and fitness of Brazil nut trees. Density responses to environmental gradients were in line with predictions under the Janzen–Connell and Herms–Mattson hypotheses, whereas tree fitness responses were in line with resource requirements of trees over their life cycle. These trade-offs resulted in divergent responses in area-based seed production and AGB. While seed production and AGB of individual trees (i.e., fitness) responded similarly to most environmental gradients, they showed opposite tendencies to tree density for almost half of the gradients. However, for gradients creating opposite fitness-density responses, area-based seed production was invariable, while trends in area-based AGB tended to mirror the response of tree density. We conclude that while the relation between environmental gradients and tree density is generally indicative of the response of AGB accumulation in a given area of forest, this is not necessarily the case for fruit production.
Tandem UGT71B5s Catalyze Lignan Glycosylation in Isatis indigotica With Substrates Promiscuity
Authors: Chen Xiao , Chen Junfeng , Feng Jingxian , Wang Yun , Li Shunuo , Xiao Ying , Diao Yong , Zhang Lei , Chen Wansheng
Published: March 2021

Lignans are a class of chemicals formed by the combination of two molecules of phenylpropanoids with promising nutritional and pharmacological activities. Lignans glucosides, which are converted from aglycones catalyzed by uridine diphosphate (UDP) glycosyltransferases (UGTs), have abundant bioactivities. In the present study, two UGTs from Isatis indigotica Fort., namely IiUGT71B5a and IiUGT71B5b, were characterized to catalyze the glycosylation of lignans with promiscuities toward various sugar acceptors and sugar donors, and pinoresinol was the preferred substrate. IiUGT71B5a was capable of efficiently producing both pinoresinol monoglycoside and diglycoside. However, IiUGT71B5b only produced monoglycoside, and exhibited considerably lower activity than IiUGT71B5a. Substrate screening indicated that ditetrahydrofuran is the essential structural characteristic for sugar acceptors. The transcription of IiUGT71B5s was highly consistent with the spatial distribution of pinoresinol glucosides, suggesting that IiUGT71B5s may play biological roles in the modification of pinoresinol in I. indigotica roots. This study not only provides insights into lignan biosynthesis, but also elucidates the functional diversity of the UGT family.
Cocktail Therapy of Fosthiazate and Cupric-Ammoniun Complex for Citrus Huanglongbing
Authors: Duan Jingwei , Li Xue , Zhang Junzhe , Cheng Baoping , Liu Shuhan , Li Hongmei , Zhou Quan , Chen Wenli
Published: March 2021

Huanglongbing (HLB) is a destructive citrus bacterial disease caused by Candidatus Liberibacter asiaticus (Ca.Las) and cannot be cured by current pesticides. Root lesion and Tylenchulus semipenetrans juveniles were observed in HLB-affected citrus tree roots. We hypothesize that root treatment with fosthiazate (FOS) and Cupric-Ammonium Complex (CAC) will improve the root growth and inhibit HLB. CAC is a broad spectrum fungicide and can promote growth of crops. FOS kills Tylenchulus semipenetrans and protects roots from damage by harmful bacteria such as Ca.Las. After 90 days of combination treatment of FOS and CAC through root drenches, the citrus grew new roots and its leaves changed their color to green. The inhibition rate of Ca.Las reached more than 90%. During treatment process, the chlorophyll content and the root vitality increased 396 and 151%, respectively, and starch accumulation decreased by 88%. Transmission electron microscopy (TEM) and plant tissue dyeing experiments showed that more irregular swollen starch granules existed in the chloroplast thylakoid system of the HLB-infected leaves. This is due to the blocking of their secretory tissue by starch. TEM and flow cytometry experiments in vitro showed the synergistic effects of FOS and CAC. A transcriptome analysis revealed that the treatment induced the differential expression of the genes which involved 103 metabolic pathways. These results suggested that the cocktail treatment of FOS and CAC may effectively kill various pathogens including Ca.Las on citrus root and thus effectively control HLB.
Seasonal Nutrient Cycling and Enrichment of Nutrient-Related Soil Microbes Aid in the Adaptation of Ramie (Boehmeria nivea L.) to Nutrient-Deficient Conditions
Authors: Wu Shenglan , Xue Shuai , Iqbal Yasir , Xing Hucheng , Jie Yucheng
Published: March 2021

The breeding for varieties tolerant of adverse growing conditions is critical for sustainable agriculture, especially for ramie (Boehmeria nivea L.). However, a lack of information on the tolerance of ramie to nutrient-deficient conditions has hindered efforts to breed ramie varieties tolerant of such conditions. The main objective of this study was to explore the tolerance strategies of ramie plants under poor soil conditions using long-term (8–9 years) field trials. Genotypes of Duobeiti 1 and Xiangzhu XB were highly tolerant of poor soil conditions. The contributions of seasonal nutrient cycling and rhizobacteria to the ability of ramie to tolerate poor soil were tested. Nitrogen and phosphorus retranslocation to the root at the end of the growing season helped ramie adapt to poor soil conditions. The contribution of the microbial community was analyzed using high-throughput Illumina MiSeq sequencing technology. The enrichment of beneficial bacteria (mainly Bradyrhizobium, Gaiella, and norank_o_Gaiellales) and the reduction of harmful fungi (mainly Cladosporium and Aspergillus) also contributed to the ability of ramie to tolerate poor soils. The results of this study provide new insight into the ability of ramie to tolerate adverse conditions and aid future efforts to breed and cultivate ramie tolerant of adverse conditions.
Evaluating and Screening of Agro-Physiological Indices for Salinity Stress Tolerance in Wheat at the Seedling Stage
Authors: Tao Rongrong , Ding Jinfeng , Li Chunyan , Zhu Xinkai , Guo Wenshan , Zhu Min
Published: March 2021

Soil salinity is a worldwide issue that affects wheat production. A comprehensive understanding of salt-tolerance mechanisms and the selection of reliable screening indices are crucial for breeding salt-tolerant wheat cultivars. In this study, 30 wheat genotypes (obtained from a rapid selection of 96 original varieties) were chosen to investigate the existing screening methods and clarify the salinity tolerance mechanisms in wheat. Ten-day-old seedlings were treated with 150 mM NaCl. Eighteen agronomic and physiological parameters were measured. The results indicated that the effects of salinity on the agronomic and physiological traits were significant. Salinity stress significantly decreased K+ content and K+/Na+ ratio in the whole plant, while the leaf K+/Na+ ratio was the strongest determinant of salinity tolerance and had a significantly positive correlation with salt tolerance. In contrast, salinity stress significantly increased Na+ concentration and relative gene expression (TaHKT1;5, TaSOS1, and TaAKT1-like). The Na+ transporter gene (TaHKT1;5) showed a significantly greater increase in expression than the K+ transporter gene (TaAKT1-like). We concluded that Na+ exclusion rather than K+ retention contributed to an optimal leaf K+/Na+ ratio. Furthermore, the present exploration revealed that, under salt stress, tolerant accessions had higher shoot water content, shoot dry weight and lower stomatal density, leaf sap osmolality, and a significantly negative correlation was observed between salt tolerance and stomatal density. This indicated that changes in stomata density may represent a fundamental mechanism by which a plant may optimize water productivity and maintain growth under saline conditions. Taken together, the leaf K+/Na+ ratio and stomatal density can be used as reliable screening indices for salt tolerance in wheat at the seedling stage.
A Sociocultural Perspective on English-as-a-Foreign-Language (EFL) Teachers’ Cognitions About Form-Focused Instruction
Authors: Sun Qiang , Zhang Lawrence Jun
Published: March 2021

There has been much research into teacher beliefs about teaching and learning as seen in the general teacher education literature. In the field of language teacher education, this line of research has been evolving, with the recent trend being streamlined into “teacher cognition” as a generic or umbrella term. Despite increasing amounts of research output so far, research into foreign language teachers’ cognitions about their own teaching and decision-making is still insufficient, particularly with regard to university-level English-as-a-foreign-language (EFL) teachers in China. Drawing on Vygotsky’s Sociocultural Theory, this qualitative research focused on EFL teachers’ cognitions about form-focused instruction in Chinese university settings. It intended to discover how teachers’ cognitions changed when they were expected to teach in actual classrooms and what factors contributed to these changes. Data collected from four teacher-participants through semi-structured interviews, classroom observations and follow-up stimulated recall interviews showed participants’ support forfocus-on-forminstruction, which means they not only paid attention to the grammatical form of the language but also to the meaning it is intended to convey. However, data also showed that the teacher-participants shifted fromfocus-on-formtofocus-on-formSinstruction in actual teaching, which suggests that they might have realized the challenges of carrying out teaching activities surroundingfocus-on-formand would like to take an easier approach by only teaching the grammar of the language byfocusing on formS. Such incongruences are interpreted with reference to a plethora of sociocultural factors including traditional Chinese thinking and institutional expectations. The implications of the findings for stakeholders in universities, including faculty members, students, and curriculum developers in similar contexts, are also discussed.
Health, Work, and Family Strain – Psychosocial Experiences at the Early Stages of Long-Term Sickness Absence
Authors: Standal Martin I. , Foldal Vegard S. , Hagen Roger , Aasdahl Lene , Johnsen Roar , Fors Egil A. , Solbjør Marit
Published: March 2021

Background

Knowledge about the psychosocial experiences of sick-listed workers in the first months of sick leave is sparse even though early interventions are recommended. The aim of this study was to explore psychosocial experiences of being on sick leave and thoughts about returning to work after 8–12 weeks of sickness absence.

Methods

Sixteen individuals at 9–13 weeks of sick leave participated in semi-structured individual interviews. Data was analyzed through Giorgi’s descriptive phenomenological method.

Results

Three themes emerged: (1) energy depleted, (2) losing normal life, (3) searching for a solution. A combination of health, work, and family challenges contributed to being drained of energy, which affected both work- and non-work roles. Being on sick leave led to a loss of social arenas and their identity as a contributing member of society. Participants required assistance to find solutions toward returning to work.

Conclusion

Even in this early stage of long-term sick leave, sick listed workers faced complex challenges in multiple domains. Continuing sick leave was experienced as necessary but may challenge personal identity and social life. Those not finding solutions may benefit from additional early follow-up that examine work-related, social and personal factors that influence return to work.
“Doing Things Together Is What It’s About”: An Interpretative Phenomenological Analysis of the Experience of Group Therapeutic Songwriting From the Perspectives of People With Dementia and Their Family Caregivers
Authors: Clark Imogen N. , Baker Felicity A. , Tamplin Jeanette , Lee Young-Eun C. , Cotton Alice , Stretton-Smith Phoebe A.
Published: March 2021

Background

The wellbeing of people living with dementia and their family caregivers may be impacted by stigma, changing roles, and limited access to meaningful opportunities as a dyad. Group therapeutic songwriting (TSW) and qualitative interviews have been utilized in music therapy research to promote the voices of people with dementia and family caregivers participating in separate songwriting groups but not together as dyads.

Procedures

This study aimed to explore how ten people with dementia/family caregiver dyads experienced a 6-week group TSW program. Dyads participated in homogenous TSW groups involving 2–4 dyads who were either living together in the community (2 spousal groups) or living separately because the person with dementia resided in a care home (1 family group, 1 spousal group). The TSW program, informed by personhood, couplehood, family centered and group process frameworks, involved creating original lyrics through song parody and song collage. Qualified Music Therapists facilitated sessions and interviewed each dyad separately. Interviews were analyzed using interpretative phenomenological analysis.

Findings

Five recurrent group themes were developed, indicating group TSW: (1) was a positive shared experience, benefiting both members of the dyad and motivating further engagement with music; (2) stimulated mental processes and reignited participants’ interests and skills; (3) provided meaningful opportunities for reflection and connection with memories and life experiences; and (4) prompted interaction and collaboration, leading to social connections, empathic relationships and experiences of inclusion. Participants also highlighted how: (5) the facilitated process supported engagement, highlighting abilities and challenging doubts.

Conclusion

Dyads identified group TSW as an opportunity to recognize strengths, voice ideas and opinions, share meaningful experiences, and do “more with music.” Participants valued TSW as a new, creative and stimulating experience that enabled connection with self and others and led to feelings of pride and achievement. Our findings further recognize how therapeutic intention and approach were reflected in participants’ engagement and responses regardless of dementia stage and type, dyad relationship, or musical background. This research may broaden perspectives and expand understanding about how people with dementia and their family caregivers access and engage in music therapy.
Perspective-Taking With Deictic Motion Verbs in Spanish: What We Learn About Semantics and the Lexicon From Heritage Child Speakers and Adults
Authors: Goldin Michele , Syrett Kristen , Sanchez Liliana
Published: March 2021

In English, deictic verbs of motion, such ascomecan encode the perspective of the speaker, or another individual, such as the addressee or a narrative protagonist, at a salient reference time and location, in the form of an indexical presupposition. By contrast, Spanish has been claimed to have stricter requirements on licensing conditions forvenir(“to come”), only allowing speaker perspective. An open question is how a bilingual learner acquiring both English and Spanish reconciles these diverging language-specific restrictions. We face this question head on by investigating narrative productions of young Spanish-English bilingual heritage speakers of Spanish, in comparison to English monolingual and Spanish dominant adults and children. We find that the young heritage speakers producevenirin linguistic contexts where most Spanish adult speakersdo not, but where English monolingual speakersdo, and also resemble those of young monolingual Spanish speakers of at least one other Spanish dialect, leading us to generate two mutually-exclusive hypotheses: (a) the encoding of speaker perspective in the young heritage children is cross-linguistically influenced by the more flexible and dominant language (English), resulting in a wider range of productions by these malleable young speakers than the Spanish grammar actually allows, or (b) the young Spanish speakers are exhibiting productions that are in fact licensed in the grammar, but which are pruned away in the adult productions, being supplanted by other forms as the lexicon is enriched. Given independent evidence of the heritage speakers' robust Spanish linguistic competence, we turn to systematically-collected acceptability judgments of three dialectal varieties of monolingual adult Spanish speakers of the distribution of perspective-taking verbs, to assess their competence and adjudicate between (a) and (b). We find that adultsaccept venirin contexts in which they do notproduceit, leading us to argue that (a)venirisnotobligatorily speaker-oriented in Spanish, as has been claimed, (b) adults may not producevenirin these contexts because they instead select more specific motion verbs, and (c) for heritage bilingual children, the more dominant language (English) may support the grammatically licensed but lexically-constrained productions in Spanish.
Extended Perspective Shift and Discourse Economy in Language Processing
Authors: Harris Jesse A.
Published: March 2021

Research spanning linguistics, psychology, and philosophy suggests that speakers and hearers are finely attuned to perspectives and viewpoints that are not their own, even though perspectival information is not encoded directly in the morphosyntax of languages like English. While some terms seem to require a perspective or a judge for interpretation (e.g., epithets, evaluative adjectives, locational PPs, etc.), perspective may also be determined on the basis of subtle information spanning multiple sentences, especially in vivid styles of narrative reporting. In this paper, I develop an account of the cues that are involved in evaluating and maintaining non-speaker perspectives, and present an economy-based discourse processing model of perspective that embodies two core principles. First, perspectives are subject to a “speaker-default,” but may shift to a non-speaker perspective if sufficient contextual cues are provided. Second, the processor follows the path of least resistance to maintaining perspective, opting to maintain the current perspective across sentences as long as the shifted perspective continues to be coherent. The predictions of the model are tested in a series of offline and online studies, manipulating the form of an attitude report and the tense of the sentence that follows. Implications for processing perspective and viewpoint in speech and narrative forms are explored.
A Space for Collaborative Creativity. How Collective Improvising Shapes ‘a Sense of Belonging’
Authors: Verneert Filip , Nijs Luc , De Baets Thomas
Published: March 2021

In this contribution, we draw on findings from a non-formal, community music project to elaborate on the relationship between the concept ofeudaimonia, as defined by Seligman, the interactive dimensions of collective free improvisation, and the concept of collaborative creativity. The project revolves around The Ostend Street Orkestra (TOSO), a music ensemble within which homeless adults and individuals with a psychiatric or alcohol/drug related background engage in collective musical improvisation. Between 2017 and 2019 data was collected through open interviews and video recordings of rehearsals and performances. Participant data was analyzed through inductive analysis based on the principles of grounded theory. One interesting finding was the discrepancy in the participant interviews between social relationships indicative of a negative affect about social group interaction versus strong feelings of group coherence and belonging. Video recordings of performances and rehearsals showed clear enjoyment and pleasure while playing music. Alongside verbal reflection through one-on-one interviews video recordings and analysis of moment-to moment observations should be used, in order to capture the complexity of community music projects with homeless people. The initial open coding was aligned with the five elements of the PERMA model. Overall, we observed more focus on Relationship (sense of belonging), Engagement (flow in rehearsals and performances) and Meaning (belonging to something greater than yourself) and less on Positive Emotion and Accomplishment (goal setting).
The Outcomes of Health Education Programme on Stress Level Among the Caregivers of Post Total Knee Replacement Surgery
Authors: Samsuddin Md. Fadlisham , Karim Jalina , Salim Azizul Akram
Published: March 2021

Introduction: Stress level among the caregivers is often related to caregivers' lack of knowledge and skill to care for the patients. A health education program to the caregivers is one of the important elements in increasing the knowledge and skills in managing patients at home. The specific objectives of this study were to determine caregiver's stress level in managing post total knee replacement (TKR) patients pre and post of a health education program.

Materials and Methods: A clinical intervention trial design was conducted in Hospital Universiti Sains Malaysia (HUSM) with a sample size of 32 caregivers. A validated Zarit Burden Interview (ZBI) questionnaire was used to measure the stress level pre and post of the health education program on the management of patients post-TKR surgery which was adopted from Fresno Surgical Hospital in California.

Results: The findings showed that there was a significant difference between pre and post level of stress (p ≤ 0.001).

Conclusion: This study revealed the positive outcome of the health education program. It reduced the stress level among the caregivers in caring for their relatives with post-TKR surgery.
Anosognosia in Mild Cognitive Impairment: Lack of Awareness of Memory Difficulties Characterizes Prodromal Alzheimer's Disease
Authors: Bastin Christine , Giacomelli Fabrice , Miévis Frédéric , Lemaire Christian , Guillaume Bénédicte , Salmon Eric
Published: March 2021

While anosognosia is often present in Alzheimer's disease, the degree of awareness of cognitive difficulties in the earlier stages, such as Mild Cognitive Impairment (MCI), is less clear. Using a questionnaire and Feeling-of-Knowing tasks, the aims of this study were (1) to test the hypothesis that anosognosia is present specifically in prodromal AD stage in patients that, owing to a more severe AD neuropathology, will rapidly progress to overt dementia and (2) to assess the neural bases of self-awareness for memory functioning. A group of 44 patients with amnestic MCI and a group of 29 healthy older participants (CTRL) performed two Feeling-of-Knowing tasks (episodic and semantic FOK) and responded to the Functional Memory Scale (MARS), also completed by one of their relatives. They underwent FDG-PET and structural MRI. The participants were followed clinically for 4 years. At the end of follow-up, 23 patients with MCI developed Alzheimer's disease (converters) and 21 patients still presented symptoms of MCI without progression (non-converters). The analyses focused on the data from inclusion stratified according to clinical status 4 years later (converters, non-converters, CTRL). On the episodic FOK task, converters patients overestimated their ability to later recognize unrecalled words and they showed prediction accuracy (Hamann coefficient) at the level of chance. No difficulty was observed in any group with the semantic FOK task. On the MARS, converters patients had a higher anosognosia score than non-converters patients and CTRL, which did not differ from each other. Correlations between self-awareness scores and neuroimaging data using small volume correction analyses in a priori regions of interest in converters indicated that inaccurate episodic FOK judgments was related to changes in brain areas that might support interpretation of retrieved content for judging the likelihood of recognition. For the MARS, the association between anosognosia and decreased gray matter density of the left inferior prefrontal cortex in converters might indicate poor inhibition over outdated personal knowledge. In amnestic MCI, anosognosia could be an early sign of neurodegeneration in brain areas that would support control mechanisms over memory representations.
Development and Evaluation of a Digital Health Intervention for Substance Use Reduction in Young Refugees With Problematic Use of Alcohol and/or Cannabis—Study Protocol for a Single-Armed Feasibility Trial
Authors: Fischer Laura Charlotte , Kölligan Vera , Wieland Nuri , Klein Michael
Published: March 2021

Background: Refugee populations are at substantial risk of developing substance use disorder (SUD) and other mental health disorders. At the same time, refugee populations face numerous barriers to accessing mental health services. Digital interventions can address some of these issues, as emerging evidence indicates that digital interventions offer an effective, low-cost alternative with high accessibility and similar efficacy as standard SUD prevention programs. As an add-on to traditional services, they further present a viable approach to the lack of personnel available for foreign language communication in preventive and therapeutic settings. In the present study, we thus aim to develop and evaluate a digital health intervention (DHI) for the reduction of substance use for young refugees with problematic use of alcohol and/or cannabis. The intervention will be implemented in a smartphone app, translated into Arabic, English, Farsi, German, and Pashto, and is to be used stand-alone. It is based on methods from cognitive behavioral therapy, contains culturally adapted elements, provides practical information on how to deal safely with alcohol and cannabis, and motivates behavior change through increased self-reflection and behavioral, cognitive, and emotional skills-training in interactive exercises.

Methods: We conduct a single-armed feasibility trial among 150 young refugees with problematic use of alcohol and/or cannabis. Participants will receive a digital screening and digital health intervention (DHI) for the reduction of problematic substance use, carried out over a 4-week time frame. The primary outcomes are the intervention's feasibility and the target population's acceptance of the intervention. The secondary outcome is a change in substance use post-intervention from baseline. Measurements are taken pre-intervention (baseline), post-intervention (after 4 weeks), and at 3- and 6-month follow-ups. We expect the intervention to be feasible and accepted by the target group.

Discussion: The present study will establish to what degree the digital intervention (the “BePrepared App”) is feasible and accepted by the target group. The evaluation of an easily accessible, feasible, and accepted intervention may impact refugees' mental health and health-related consequences. It aims at overcoming barriers to preventive health care in the substance use field for underserved refugee populations as a tool within a larger set of urgently needed interventions.

Trial Registration: DRKS00021095 at the “German Clinical Trials Register” (30.03.2020).
Vasculogenic Mimicry in Head and Neck Squamous Cell Carcinoma—Time to Take Notice
Authors: Salem Abdelhakim , Salo Tuula
Published: March 2021

Head and neck squamous cell carcinoma (HNSCC) is a group of common cancers characterized by a swift growth pattern, early metastasis, and dismal 5-year survival rates. Despite the recent advances in cancer management, the multimodality approach is not effective in eradicating HNSCC. Moreover, the clinical response to the antiangiogenic therapy remains considerably limited in HNSCC patients, suggesting that tumor perfusion can take place through other non-angiogenic pathways. Tumor cell-induced angiogenesis is one of the main hallmarks of cancer. However, at the end of the previous millennium, a new paradigm of tumor cell-associated neovascularization has been reported in human melanoma cells. This new phenomenon, which was named “vasculogenic mimicry” or “vascular mimicry” (VM), describes the ability of aggressively growing tumor cells to form perfusable, matrix-rich, vessel-like networks in 3-dimensional matrices in vitro. Similar matrix-rich VM networks were also identified in tissue samples obtained from cancer patients. To date, myriad studies have reported intriguing features of VM in a wide variety of cancers including HNSCC. We aim in this mini-review to summarize the current evidence regarding the phenomenon of VM in HNSCC—from the available detection protocols and potentially involved mechanisms, to its prognostic value and the present limitations.
Alternative Seafood Networks During COVID-19: Implications for Resilience and Sustainability
Authors: Stoll Joshua S. , Harrison Hannah L. , De Sousa Emily , Callaway Debra , Collier Melissa , Harrell Kelly , Jones Buck , Kastlunger Jordyn , Kramer Emma , Kurian Steve , Lovewell M. Alan , Strobel Sonia , Sylvester Tracy , Tolley Brett , Tomlinson Andrea , White Easton R. , Young Talia , Loring Philip A.
Published: March 2021

Export-oriented seafood trade faltered during the early months of the COVID-19 pandemic. In contrast, alternative seafood networks (ASNs) that distribute seafood through local and direct marketing channels were identified as a “bright spot.” In this paper, we draw on multiple lines of quantitative and qualitative evidence to show that ASNs experienced a temporary pandemic “bump” in both the United States and Canada in the wake of supply chain disruptions and government mandated social protections. We use a systemic resilience framework to analyze the factors that enabled ASNs to be resilient during the pandemic as well as challenges. The contrast between ASNs and the broader seafood system during COVID-19 raises important questions about the role that local and regional food systems may play during crises and highlights the need for functional diversity in supply chains.
Cecal Microbiota Modulates Fat Deposition in Muscovy Ducks
Authors: Lyu Wentao , Liu Xiuting , Lu Lizhi , Dai Bing , Wang Wen , Yang Hua , Xiao Yingping
Published: March 2021

Ducks with the same genetic background vary greatly in their adiposity phenotypes. The gut microbiota plays an essential role in host physiological development and metabolism including fat deposition. However, the association of the gut microbiota with the lipogenic phenotype of ducks remains unknown. In this study, we investigated the cecal microbiota of adult Muscovy ducks and the correlation of the cecal microbiota with fat phenotypes. A total of 200 Muscovy ducks were selected from a population of 5,000 Muscovy ducks to record their abdominal fat weight and collect their cecal contents after being slaughtered and defeathered. The cecal contents were subjective to DNA isolation and 16S rRNA gene sequencing. The results were sorted according to the percentage of abdominal fat and the top 20% (n = 40) and the bottom 20% (n = 40) were set as the high and low groups, respectively. Our results indicated that in the cecum of Muscovy ducks, Bacteroidetes, Firmicutes, and Fusobacteria were the predominant phyla while Bacteroides, Oscillospiraceae_uncultured, Parabacteroides, and Bacteroidales_norank were the top 4 dominant genera. Abdominal fat weight (18.57~138.10 g) and percentage of abdominal fat (1.02~27.12%) were significantly correlated (R2 = 0.92, P < 0.001). Although the lipogenic phenotypes of ducks had a significant difference (P < 0.05), the α-diversities of the high and low groups were not significantly different (P > 0.05). Nevertheless, after random forest analysis, we identified two genera, Treponema and Ruminococcus_torques_group, that were significantly associated with fat deposition in Muscovy ducks. In addition, the abundances of Treponema and Ruminococcus_torques_group gave a significantly negative and positive association with abdominal fat weight, respectively (P < 0.05). Ducks with a low level of Treponema exhibited a tendency toward a high percentage of abdominal fat (P < 0.01), while the percentage of abdominal fat in ducks with high Ruminococcus_torques_group abundance tended to be higher than that in ducks with low Ruminococcus_torques_group abundance (P < 0.01). These findings could provide the basic data on the cecal microbiota in Muscovy ducks as well as a theoretical foundation to limit the fat deposition by modulating the gut microbiota in the duck industry.
Bovine Coronavirus and the Associated Diseases
Authors: Vlasova Anastasia N. , Saif Linda J.
Published: March 2021

Coronaviruses (CoVs) possess the largest and most complex RNA genome (up to 32 kb) that encodes for 16 non-structural proteins regulating RNA synthesis and modification. Coronaviruses are known to infect a wide range of mammalian and avian species causing remarkably diverse disease syndromes. Variable tissue tropism and the ability to easily cross interspecies barriers are the well-known characteristics of certain CoVs. The 21st century epidemics of severe acute respiratory CoV (SARS-CoV), Middle East respiratory CoV and the ongoing SARS-CoV-2 pandemic further highlight these characteristics and emphasize the relevance of CoVs to the global public health. Bovine CoVs (BCoVs) are betacoronaviruses associated with neonatal calf diarrhea, and with winter dysentery and shipping fever in older cattle. Of interest, no distinct genetic or antigenic markers have been identified in BCoVs associated with these distinct clinical syndromes. In contrast, like other CoVs, BCoVs exist as quasispecies. Besides cattle, BCoVs and bovine-like CoVs were identified in various domestic and wild ruminant species (water buffalo, sheep, goat, dromedary camel, llama, alpaca, deer, wild cattle, antelopes, giraffes, and wild goats), dogs and humans. Surprisingly, bovine-like CoVs also cannot be reliably distinguished from BCoVs using comparative genomics. Additionally, there are historical examples of zoonotic transmission of BCoVs. This article will discuss BCoV pathogenesis, epidemiology, interspecies transmission, immune responses, vaccines, and diagnostics.
Bit-Related Lesions in Event Horses After a Cross-Country Test
Authors: Tuomola Kati , Mäki-Kihniä Nina , Valros Anna , Mykkänen Anna , Kujala-Wirth Minna
Published: March 2021

Bit-related oral lesions are common and may impair horse welfare. The aim of this study was to investigate the prevalence of oral lesions and their risk factors in a sample of Finnish event horses. The rostral part of the oral cavity (the bit area) of 208 event horses (127 warmbloods, 52 coldbloods, and 29 ponies) was examined in a voluntary inspection after the last competition phase, i.e., the cross-country test. Acute lesions were observed in 52% (109/208) of the horses. The lesion status was graded as no acute lesions for 48% (99/208), mild for 22% (45/208), moderate for 26% (55/208) and severe for 4% (9/208) of the horses. The inner lip commissure was the most common lesion location observed in 39% (81/208) of the horses. A multivariable logistic regression model with data of 174 horses was applied to risk factor analysis. Horses wearing thin (10–13 mm) (OR 3.5, CI 1.4–8.7) or thick (18–22 mm) (OR 3.4, CI 1.4–8.0) bits had a higher risk of moderate/severe lesion status than horses wearing middle-sized (14–17 mm) bits (P = 0.003). Breed was associated with moderate/severe lesion status (P = 0.02). The risk was higher for warmbloods (reference group) and coldbloods (OR 2.0, CI 0.88–4.7) compared with ponies (OR 0.2, CI 0.04–0.87). Mares were at higher risk of moderate/severe lesion status (OR 2.2, CI 1.1–4.5) than geldings (reference group) (P = 0.03). Bar lesions were more common in horses with unjointed bits (40%, 8/20) than with basic double-jointed (10%, 5/52), formed double-jointed (8%, 6/78) or single-jointed bits (5%, 2/40) (Fisher's exact test, P = 0.002). The results of this study suggest that thin and thick bits and mare sex should be considered risk factors for mouth lesions. In addition, in this sample ponies had smaller risk for lesions than other horse breeds. We encourage adopting bit area monitoring as a new routine by horse handlers and as a welfare measure by competition organizers for randomly drawn horses.
Targeting UCHL1 Induces Cell Cycle Arrest in High-Risk Multiple Myeloma with t(4;14)
Authors: Kamseng Parin , Siriboonpiputtana Teerapong , Puavilai Teeraya , Chuncharunee Suporn , Paisooksantivatana Karan , Chareonsirisuthigul Takol , Junking Mutita , Chiraphapphaiboon Wannasiri , Yenchitsomanus Pa-thai , Rerkamnuaychoke Budsaba
Published: March 2021

Multiple myeloma (MM) patients considered to be at high cytogenetic risk commonly fail to respond to standard treatment. A thorough understanding of the molecular mechanism of MM development is, therefore, needed. We endeavored to explore the transcriptional signature among different subgroups of newly diagnosed MM using gene chip-based expression microarray. Bone marrow samples of 15 newly diagnosed Thai MM patients were included. The chromosomal translocation t(4;14) was the most frequently identified genetic alteration in the high-risk subgroup. Cluster analysis from expression profiling demonstrated that high-risk MM have a distinctly different expression pattern compared to standard-risk patients. The most significant differentially expressed gene was UCHL1. Functional enrichment analysis by Gene Set Enrichment Analysis, FUNRICH, and Gene Ontology Panther pathway revealed the gene sets involved in cell cycle control to be enriched in the t(4;14) high-risk group. Interestingly, among the well-established downstream targets of UCHL1, only CCND2 was significantly expressed in the t(4;14) high-risk group. Suppression of UCHL1 protein level by LDN-5744 inhibitor could arrest the cell cycle in G1 phase in cell lines. These findings shed light on the molecular mechanism of UCHL1 in t(4;14) high-risk MM and support the evidence that alteration of the UCHL1 pathway may play a role in the pathogenesis of high-risk MM.
HMGB1 Translocation is Associated with Tumor-Associated Myeloid Cells and Involved in the Progression of Fibroblastic Sarcoma
Authors: Chen Huoying , Lin Xiaoying , Liu Hongbo , Huang Cheng , Li Rong , Ai Jie , Wei Jiaxue , Xiao Shengjun
Published: March 2021

The morphological variability and genetic complexity of fibroblastic sarcoma makes its diagnosis and treatment a challenge. High-mobility group box 1 protein (HMGB1), which functions as a DNA chaperone and a prototypical damage-associated molecular pattern, plays a paradoxical role in cancer. However, the expression pattern and role of HMGB1 in fibroblastic sarcomas is ill defined. By immunostaining of 95 tissue microarray cores of fibroblastic sarcomas, HMGB1 was found to be expressed in most tumor tissues. Nuclear HMGB1 translocation to cytoplasm was observed both in tumor cells and vascular endothelial cells. A visible number of tumor-associated myeloid cells including CD68+ and CD163+ macrophages and CD33+ myeloid cells were also detected in most tumor tissues. HMGB1 translocation was not only associated with CD68, CD163, and CD33 density, but also with disease progression. These results imply that HMGB1, an important regulator of the tumor microenvironment, is associated with tumor-associated myeloid cells and involved in the progression of fibroblastic sarcomas; HMGB1 may serve as a promising prognostic biomarker and a potential therapeutic target for fibroblastic sarcoma.
Short Communication: Detecting Heavy Goods Vehicles in Rest Areas in Winter Conditions Using YOLOv5
Authors: Kasper-Eulaers Margrit , Hahn Nico , Berger Stian , Sebulonsen Tom , Myrland Øystein , Kummervold Per Egil
Published: March 2021

The proper planning of rest periods in response to the availability of parking spaces at rest areas is an important issue for haulage companies as well as traffic and road administrations. We present a case study of how You Only Look Once (YOLO)v5 can be implemented to detect heavy goods vehicles at rest areas during winter to allow for the real-time prediction of parking spot occupancy. Snowy conditions and the polar night in winter typically pose some challenges for image recognition, hence we use thermal network cameras. As these images typically have a high number of overlaps and cut-offs of vehicles, we applied transfer learning to YOLOv5 to investigate whether the front cabin and the rear are suitable features for heavy goods vehicle recognition. Our results show that the trained algorithm can detect the front cabin of heavy goods vehicles with high confidence, while detecting the rear seems more difficult, especially when located far away from the camera. In conclusion, we firstly show an improvement in detecting heavy goods vehicles using their front and rear instead of the whole vehicle, when winter conditions result in challenging images with a high number of overlaps and cut-offs, and secondly, we show thermal network imaging to be promising in vehicle detection.
Dynamic Initial Weight Assignment for MaxSAT
Authors: Ishtaiwi Abdelraouf , Abu Al-Haija Qasem
Published: March 2021

The Maximum Satisfiability (Maximum Satisfiability (MaxSAT)) approach is the choice, and perhaps the only one, to deal with most real-world problems as most of them are unsatisfiable. Thus, the search for a complete and consistent solution to a real-world problem is impractical due to computational and time constraints. As a result, MaxSAT problems and solving techniques are of exceptional interest in the domain of Satisfiability (Satisfiability (SAT)). Our research experimentally investigated the performance gains of extending the most recently developed SAT dynamic Initial Weight assignment technique (InitWeight) to handle the MaxSAT problems. Specifically, we first investigated the performance gains of dynamically assigning the initial weights in the Divide and Distribute Fixed Weights solver (DDFW+Initial Weight for Maximum Satisfiability (DDFW+InitMaxSAT)) over Divide and Distribute Fixed Weights solver (DDFW) when applied to solve a wide range of well-known unweighted MaxSAT problems obtained from DIMACS. Secondly, we compared DDFW+InitMaxSAT’s performance against three known state-of-the-art SAT solving techniques: YalSAT, ProbSAT, and Sparrow. We showed that the assignment of dynamic initial weights increased the performance of DDFW+InitMaxSAT against DDFW by an order of magnitude on the majority of problems and performed similarly otherwise. Furthermore, we showed that the performance of DDFW+InitMaxSAT was superior to the other state-of-the-art algorithms. Eventually, we showed that the InitWeight technique could be extended to handling partial MaxSAT with minor modifications.
Reduced Vegetative Growth Increases Grain Yield in Spring Wheat Genotypes in the Dryland Farming Region of North-West China
Authors: Feng Tao , Xi Yue , Zhu Yong-He , Chai Ning , Zhang Xin-Tan , Jin Yi , Turner Neil C. , Li Feng-Min
Published: March 2021

To understand the co-evolution in yield-related traits with the breeding, selection, and introduction of genotypes for increased grain yield, field experiments were carried out at two sites in the western area of the Loess Plateau in China that differed in hydrothermal conditions. Sixteen genotypes of spring wheat introduced and grown over the past 120 years were compared in terms of their yield and yield-related traits. As the grain yield increased, the spike number per unit area and the grain number per spike increased linearly, but the 1000-kernel weight was not correlated with grain yield. In the more recent genotypes, anthesis was initiated significantly earlier, although the length of the period from anthesis to maturity remained unchanged. Water use and the Effective Use of Water (EUW) for aboveground biomass before anthesis and the contribution of pre-anthesis aboveground biomass to grain yield all decreased as grain yield increased. Soil water content at anthesis was negatively correlated with aboveground biomass at anthesis, but positively correlated with grain yield. Conclusively, breeding in spring wheat over the past century has increased the yield of new genotypes by (1) increasing the number of grains per unit area; (2) shortening the period of vegetative growth; (3) decreasing EUW and the soil water use before anthesis; thereby (4) retaining more soil water and increasing biomass accumulation after anthesis. Future spring wheat breeding for this dryland region should determine whether the time for grain filling from anthesis to maturity can be extended to enable greater use of environmental resources and higher yields.
Phytoavailability of Cd, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Tl and Zn in Arable Crop Systems Amended for 13 to 15 Years with Organic Waste Products
Authors: Michaud Aurélia Marcelline , Sappin-Didier Valérie , Cambier Philippe , Nguyen Christophe , Janot Noémie , Montenach Denis , Filipovic Lana , Deltreil Valentin , Houot Sabine
Published: March 2021

Repeated applications of organic waste products (OWP) are a source of trace elements (TE) inputs to agricultural topsoils. The present study aimed at (i) assessing the effects of repeated OWP inputs on the chemical properties of topsoils in two long-term field experiments (13 and 15 years; calcareous and non-calcareous soils), (ii) evaluating TE phytoavailability and their transfer to grain (winter wheat and maize) and (iii) identifying the underlying factors causing alterations of TE phytoavailability. In both field experiments, receiving compliant or slightly high doses of OWP in compliance with regulations, OWP and soil physicochemical properties and TE concentrations in soils and grains were determined. In situ phytoavailability of TE was assessed at two juvenile crop growth stages by analyzing TE concentrations in shoot plantlets. Depending on the OWP input amount, results showed that compared to the soil receiving no organic amendment, repeated OWP inputs significantly increased soil organic carbon content, pH, cation exchange capacity, total soil Cu, Mo and Zn concentration and the phytoavailability of Mo, while the phytoavailability of Cd, Mn, Ni and Tl was significantly reduced. No notable effect was observed for Cr, Cu, Hg, Pb and Zn phytoavailability. Statistical approaches suggested that due to the repeated OWP applications, increased soil organic carbon content and pH, were likely responsible for decreased TE phytoavailability (e.g., Cd).