bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of a home‐used high‐intensity focused ultrasound device on wrinkle reduction
Authors: Kwack Mi Hee , Lee Weon Ju
Published: December 2022

Background

High‐intensity focused ultrasound (HIFU) has been developed for the treatment of skin wrinkles on the face, neck, and body.

Objectives

This study aimed to evaluate the effects of a home‐used HIFU device on wrinkles in mice based on the expression of fibrosis‐related genes and proteins.

Methods

The backs of 20‐week‐old mice were treated with a home‐used HIFU using the following probes: 4 MHz, 1.5 mm focal depth. The treated mice were compared with young mice by histological examination, real‐time polymerase chain reaction (PCR), and immunohistochemistry. Histological examination was performed by trichrome staining. Real‐time PCR and immunohistochemistry were conducted to determine the expression of collagen types I and III, matrix metalloproteinase (MMP)‐1, and tissue inhibitor of metalloproteinase (TIMP)‐1.

Results

Dermal thickness was increased after treatment with the home‐used HIFU device at 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks on trichrome. Gene and protein expression of collagen types I and III and elastin were increased after treatment with HIFU at all options of 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks. Gene and protein expressions of MMP‐1 and TIMP‐1 were decreased after treatment with HIFU device at 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks.

Conclusion

The home‐used HIFU device can be an effective therapeutic modality for skin tightening.
Identification of the novel HLA‐DPA1*02:89 allele using next‐generation sequencing
Authors: Choi Heekang , Hong Jun Young , Jung Sunmi , Lee Jina , Park Younhee
Published: December 2022

The HLA‐DPA1*02:89 allele differs from HLA‐DPA1*02:02:02:01 allele by a single nucleotide in codon 224.
From Athens to Cardiff to Corwen: Ministerial formation in the real world
Authors: James Manon Ceridwen
Published: December 2022

This article explores the delivery and pedagogy of initial ministerial training for lay and ordained candidates in the Church in Wales by its training arm, the St. Padarn's Institute. I discuss how logistical challenges force us into a more pragmatic approach where practical considerations are as influential as philosophical and pedagogical commitments. The close relationship between the national church and ourselves and a “reflective practice” and “practical wisdom” approach enables us to be responsive and flexible to training needs. Although wary of geographical locations being used as shorthand theoretical terms for pedagogies (e.g., Athens, Berlin), I describe our approach as Athens character formation but ultimately locate our pedagogy (following Cronshaw) within the lived experience and landscape of Wales.
Framework for refugee and migrant health research in the WHO European Region
Authors: MacFarlane Anne , Puthoopparambil Soorej Jose , Waagensen Elisabeth , Sisti Leuconoe Grazia , Costanzo Gianfranco , Kayi Ilker , Osseiran Souad , Sakarya Sibel , Severoni Santino , Hannigan Ailish
Published: December 2022

Migration is a longstanding, growing global phenomenon. As a social determinant of health, migration can lead to health inequities between people on the move and host populations. Thus, it is imperative that there is a coordinated effort to advance migration‐ and health‐related goals. WHO has a specific remit to support evidence‐based decision‐making in its Member States. As part of that remit, WHO Europe presents this Framework for Refugee and Migrant Health Research in the WHO European Region. It is designed as a starting point for debating and analysing a broad range of options and approaches to help inform a WHO global research agenda on health and migration. This is important because refugee and migrant health research is a complex interdisciplinary field that is expanding in a fast‐changing socio‐political environment. The Framework is intended for all stakeholders involved: academic, civil society organisations, refugees, migrants, policy‐makers, healthcare providers, educators and funders. It is developed by academics in consultation with these stakeholder groups. It reflects on three specific interrelated dynamics in research practice. These are (i) research prioritisation; (ii) study samples and (iii) research design. The Framework offers recommendations to consider for each one of these. It elucidates the value of involving refugees and migrants in research and research agendas and the need to develop an ecosystem that will support and sustain participatory, interdisciplinary, transdisciplinary and inter‐sectoral projects.
Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis
Authors: Zhou Yanli , Fan Wei , Zhang Haoyue , Zhang Jingling , Zhang Guanghui , Wang Ding , Xiang Guisheng , Zhao Changhong , Li Lianhua , He Simei , Lu Yingchun , Zhao Jiuxia , Meng Zhengui , Zhang Xianmin , Meng Hengling , Yin Xinhua , Yang Shengchao , Long Guangqiang
Published: December 2022

Marsdenia tenacissima is a medicinal plant widely distributed in the calcium‐rich karst regions of southwest China. However, the lack of a reference genome has hampered the implementation of molecular techniques in its breeding, pharmacology and domestication. We generated the chromosome‐level genome assembly in Apocynaceae using combined SMRT sequencing and Hi‐C. The genome length was 381.76 Mb, with 98.9% of it found on 11 chromosomes. The genome contained 222.63 Mb of repetitive sequences and 21 899 predicted gene models, with a contig N50 of 6.57 Mb. Phylogenetic analysis revealed that M. tenacissima diverged from Calotropis gigantea at least 13.43 million years ago. Comparative genomics showed that M. tenacissima underwent ancient shared whole‐genome duplication. This event, together with tandem duplication, contributed to 70.71% of gene‐family expansion. Both pseudogene analysis and selective pressure calculations suggested calcium‐related adaptive evolution in the M. tenacissima genome. Calcium‐induced differentially expressed genes (DEGs) were mainly enriched in cell‐wall‐related processes. Domains (e.g. Fasciclin and Amb_all) and cis‐elements (e.g. MYB and MYC) frequently occurred in the coding and promoter regions of cell‐wall DEGs, respectively, and the expression levels of these genes correlated significantly with those of calcium‐signal‐related transcription factors. Moreover, calcium addition increased tenacissoside I, G and H contents. The availability of this high‐quality genome provides valuable genomic information for genetic breeding and molecular design, and lends insights into the calcium adaptation of M. tenacissima in karst areas.
Biased mutations and gene losses underlying diploidization of the tetraploid broomcorn millet genome
Authors: Sun Yanling , Liu Yang , Shi Jinfeng , Wang Lun , Liang Chengzhi , Yang Jun , Chen Jinfeng , Chen Mingsheng
Published: December 2022

Broomcorn millet (Panicum miliaceum L.) is one of the earliest domesticated crops, and is a valuable resource to secure food diversity and combat drought stresses under the global warming scenario. However, due to the absence of extant diploid progenitors, the polyploidy genome of broomcorn millet remains poorly understood. Here, we report the chromosome‐scale genome assembly of broomcorn millet. We divided the broomcorn millet genome into two subgenomes using the genome sequence of Panicum hallii, a diploid relative of broomcorn millet. Our analyses revealed that the two subgenomes diverged at ~4.8 million years ago (Mya), while the allotetraploidization of broomcorn millet may have occurred about ~0.48 Mya, suggesting that broomcorn millet is a relatively recent allotetraploid. Comparative analyses showed that subgenome B was larger than subgenome A in size, which was caused by the biased accumulation of long terminal repeat retrotransposons in the progenitor of subgenome B before polyploidization. Notably, the accumulation of biased mutations in the transposable element‐rich subgenome B led to more gene losses. Although no significant dominance of either subgenome was observed in the expression profiles of broomcorn millet, we found the minimally expressed genes in P. hallii tended to be lost during diploidization of broomcorn millet. These results suggest that broomcorn millet is at the early stage of diploidization and that mutations likely occurred more on genes that were marked with lower expression levels.
Blood feeding patterns of the Culex pipiens complex in equestrian land uses and their implications for arboviral encephalitis risk in temperate Argentina
Authors: Cardo María Victoria , Carbajo Aníbal Eduardo , Mozzoni Constanza , Kliger Marlene , Vezzani Darío
Published: December 2022

Blood feeding patterns of mosquitoes are a key component in the dynamics of arboviral encephalitides transmission. In temperate Argentina, the members of the Culex pipiens complex include Cx. pipiens molestus, Cx. quinquefasciatus and their hybrids. To characterize their blood feeding patterns, adult resting mosquitoes were collected monthly during the warm season in urban and rural equestrian fields. The availability of birds and domestic mammals per site was characterized. The blood source and the complex member were successfully identified for 89 specimens using PCR. Blood of 19 vertebrate species was isolated including four mammals (most common feeds from dog, Canus lupus 19% of the blood meals; and horse, Equus caballus 18%) and 15 birds (picazuro pigeon, Patagioenas picazuro 11%; eared dove, Zenaida auriculata 10%; chicken, Gallus gallus 9%). The Forage Ratio (FR), calculated as the proportion of feeds taken from a given host species with respect to that host availability in the environment, suggested preference for dog by all members of the complex (FR ≥4.5). On the contrary, FR values suggested avoidance for horse by Cx. quinquefasciatus and the hybrid (FR ≤0.8), and a use proportional to its abundance by Cx. pipiens molestus (FR = 1.1–1.2 in urban and rural sites, respectively). FR values suggesting preference were obtained for avian species of the orders Passeriformes (7 species in total) and Columbiformes (5) by all members of the complex (FR ≥ 3.3), whereas values for monk parakeet (Myiopsitta monachus, Psiitaciformes) suggested avoidance by Cx. quinquefasciatus in urban sites (FR = 0.4) and by Cx. pipiens molestus in rural sites (FR = 0.3) but not in urban sites (FR = 1.4). A mammal‐bird index (MBI, from −1 all avian to +1 all mammalian blood meals) was calculated for each member of the complex and urbanization category. Values were negative for Cx. quinquefasciatus (MBIurban = −0.60, MBIrural = −0.33) and positive for Cx. pipiens molestus (MBIurban = 0.20, MBIrural = 0.60), indicating a higher proportion of feeds taken on birds and mammals, respectively, regardless of the urbanization category. In temperate Argentina, the members of the Cx. pipiens complex fed both on horses and on birds, thus representing a real risk of transmission of arboviral encephalitides from avian enzootic cycles to horse epizootics.
Precise Measurements Using a Smartphone’s Magnetometer—Measuring Magnetic Fields and Permeability
Authors: Wannous Jarier , Horvath Peter
Published: December 2022

Measuring permeability in a high school physics course has long been a hard task. However, with the advent of using smartphones in the classroom, it is not only possible but even easily done. This paper offers detailed instructions on how to measure permeability using a smartphone’s magnetometer, starting with experimentally discovering the equation for measuring the magnetic field of a coil with a negligible length. All discussed activities and experiments can be implemented with a focus on inquiry.
Visualizing Vision
Authors: Blagotinšek Ana Gostinčar
Published: December 2022

Two misconceptions about the mechanism of image formation in the human eye are common among students and even in textbooks and other teaching materials. The first attributes all refraction to the eye lens; the second treats the eye as a pinhole camera. To reduce these persistent conceptions of students, a series of simple experiments is presented using a water-filled fishbowl as a model of the eye. The model is used to demonstrate the propagation of light through the eye and the image formation on the retina in normal vision, nearsightedness, and farsightedness. The model also allows visualization of the corrections of malfunctions of the eye.
Determination of the Transverse Width and Distance of an Object with a Smartphone Camera
Authors: Sarkar Soumen , Pal Sanjoy Kumar , Chakrabarti Surajit
Published: December 2022

A smartphone is a powerful learning aid in the hands of a large section of students around the world. The camera of the phone can be used for several learning purposes apart from its obvious purpose of photographing. If the focal length of the lens of the camera can be determined, several experiments in optics can be performed with it. In some recent works, the method for determination of the focal length has been discussed. When a real image of an object is formed by a lens of known focal length, one can determine either the distance or the transverse magnification of the object if the other is known. In this work, we have shown that we can determine both the transverse size and the distance of an object, by photographing it from two positions, separated by a distance along the line of sight of the camera. In a few other works, the smartphone camera has been used for conducting experiments in optics.
Results From South Korean 2022 Para Report Card on Physical Activity for Children and Adolescents With Disabilities
Authors: Lee Jeongmin , Oh Kitaek , Min Jihee , Goo Seon-Young , Lee Eun-Young , June Yi Kyoung , Heo Jinmoo , Lee Joon-Sung , Kim Dong-il , Shin Wonsang , Kim Kwon-il , Kim Yeonsoo , Jeon Justin Y.
Published: December 2022

South Korea has developed its first Para Report Card on physical activity (PA) for children and adolescents with disabilities. Five national surveillance databases were used to evaluate PA indicators based on the benchmarks and grading rubric provided by Active Healthy Kids Global Alliance. Report card evaluation committees were invited to grade and assess the results using strengths, weaknesses, opportunities, and threats analysis. Five indicators (overall PA, D+; organized sports and PA, D−; active transportation, D−; physical fitness, D+; and government, A+) and one additional indicator (sleep, C−) were assigned a letter grade. The other five indicators were graded as incomplete. The Para Report Card revealed a significant gap between the behavioral-indicator grades (D− to D+) and the policy-indicator grade (A+), suggesting that government strategies and investment have not yet been translated into behavioral PA among children and adolescents with disabilities.
Synthesis, spectroscopic, and electronic properties of new tetrapyrazinoporphyrazines with eight peripheral 2,6-diisopropylphenol groups
Authors: Marx Benjamin , Schrage Briana R. , Ziegler Christopher J. , Nemykin Victor N.
Published: December 2022

Metal-free, magnesium, titanyl, and vanadyl tetrapyrazinoporphyrazines substituted with eight 2,6-diisopropylphenoxy groups at the peripheral positions were prepared and characterized by NMR, UV-Vis, magnetic circular dichroism (MCD), and mass spectrometry methods. In addition, the Pc(2,6iPrPhO)8VO complex was characterized by EPR spectroscopy and X-ray crystallography. Reaction between TiCl4 with 4,5-(2,6-diisopropylphenoxy)phthalonitrile in N,N-dimethylaminoethanol resulted in the formation of a red open-chain trimer, which was characterized by mass spectrometry and X-ray crystallography. Electronic structures of new compounds and their excited state properties were probed by Density Functional Theory (DFT) and Time-Dependent DFT (TDDFT) methods.
Greener shades of blue: Green chemistry as an opportunity to improve the approach to the synthesis of phthalocyanines
Authors: Zanotti Gloria , Mancini Laura , Paoletti Anna Maria , Pennesi Giovanna , Raglione Venanzio
Published: December 2022

Green chemistry has emerged as a powerful tool to rationally design environmentally sustainable chemical processes by choosing safe chemicals and minimizing the production of waste. This article focuses on the opportunity, yet underexplored, to evaluate the green syntheses of phthalocyanines by means of metrics such as the E-factor and EcoScale, in a view to better assess their sustainability and provide insights on their viability with respect to standard procedures.
Sonicated Extract from the Aril of Momordica Cochinchinensis Inhibits Cell Proliferation and Migration in Aggressive Prostate Cancer Cells
Authors: Chainumnim Seksom , Suksamrarn Sunit , Jarintanan Faongchat , Jongrungruangchok Suchada , Wannaiampikul Sivaporn , Tanechpongtamb Wanlaya
Published: December 2022

Momordica cochinchinensis or gac fruit has been reported to have several biological activities, including antioxidation, anti-inflammatory, and anticancer activities. However, the effect on cancer cell metastasis has not been extensively studied. With this aim, the extract from the aril part was selected and investigated for prostate cancer cell migration. The aril extracts were prepared as boiled extract, sonicated extract, ethanol extract, and HAE (hexane:acetone:ethanol; 2 : 1 : 1) extract, while the prostate cancer cell models were PC-3 and LNCaP cells. An MTT assay was performed to compare the antiproliferative effect between prostate cancer cells and normal Vero cells. As a result, the sonicated extract had the highest efficiency in PC-3 cells, with IC50 values of 2 mg/mL and 0.59 mg/mL for 48 and 72 h, respectively, while it had less of an effect in LNCaP cells and was not toxic to normal cells. Cell damage was further confirmed using LDH and cell cycle analysis. As a result, the sonicated extract did not cause cell damage or death and only inhibited cell proliferation. The effect on cancer metastasis was further examined by wound healing, transwell migration assays, and western blotting. The results demonstrated that the sonicated extract inhibited PC-3 cell migration and decreased MMP-9 but increased TIMP-1 expression. All these results support that gac fruit is a valuable source for further development as an anticancer agent for prostate cancer patients.
Predictive Value of Heart-Type Fatty Acid-Binding Protein for Mortality Risk in Critically Ill Patients
Authors: Zhang Ye , Wang Jia , Yu Bao-Zhong , Chen Mao-Lin , Cao Yu-Dan , Wei Bing , Wang Jun-Yu
Published: December 2022

Objective. Our study assessed the predictive value of heart-type fatty acid-binding protein (H-FABP) for critically ill patients. Methods. 150 critically ill patients admitted to the emergency department of Beijing Chaoyang Hospital, Capital Medical University, were included in our study from August 2021 to April 2022. Serum H-FABP, procalcitonin (PCT), lactate (LAC), and other markers were determined within 1 h after admission. The Sequential Organ Failure Assessment (SOFA) score and the Acute Physiology and Chronic Health Evaluation II (APACHE II) were calculated. The independent predictors of 28-day mortality in critically ill patients were analyzed by logistic regression, and the receiver operating characteristic curve (ROC) was used to analyze the predictive value for 28-day mortality in critically ill patients. Results. Age, APACHE II, SOFA, GCS, LAC, H-FABP, IL-6, Scr, and D-dimer were significantly different in the nonsurvivor vs. survivor groups (

P
<
0.05

), with H-FABP correlating with cTNI, Scr, PCT, and SOFA scores (

P
<
0.05

). Logistic regression analysis showed that H-FABP, APACHE II, LAC, and age were independent predictors for 28-day mortality in critically ill patients (

P
<
0.05

). The AUC of ROC curve in H-FABP was 0.709 (sensitivity 72.9%, specificity 66.1%, and cut-off 4.35), which was slightly lower than AUC of ROC curve in LAC (AUC 0.750, sensitivity 58.3%, specificity 76.1%, and cut-off 1.95) and APACHE II (AUC 0.731, sensitivity 77.1%, specificity 58.7%, and cut-off 12.5). However, statistically, there was no difference in the diagnostic value of H-FABP compared with the other two indicators (

Z

1

=
0.669

,

P
=
0.504

;

Z

2

=
0.383

,

P
=
0.702

). But H-FABP (72.9%) has higher sensitivity than LAC (58.3%). The combined evaluation of H-FABP+APACHE II score (AUC 0.801, sensitivity 71.7%, and specificity 78.2%;

Z
=
2.612

,

P
=
0.009

) had better diagnostic value than H-FABP alone and had high sensitivity (71.7%) and specificity (78.2%). Conclusion. H-FABP, LAC, APACHE II, and age can be used as independent risk factors affecting the prognosis of critically ill patients. Compared with using the above indicators alone, the H-FABP+APACHE II has a high diagnostic value, and the early and rapid evaluation is particularly important for the adjustment of treatment plans and prognosis.
Experimental Study on Overlying Strata Movement Characteristics and Distributed Optical Fiber Characterization of Stope
Authors: Chai Jing , Tian Zhicheng , Ouyang Yibo , Liu Yongliang , Zhang Dingding , Yang Jianfeng
Published: December 2022

In order to study the migration characteristics and strata behavior law of mining-induced rocks in fully mechanized caving face of coal mine, taking the actual geological mining of Longwanggou Coal Mine as the background, using computer software (KSPB) to identify the location of key strata. The physical similar material simulation test was used to monitor the movement characteristics of mining-induced rocks by using the internal displacement sensor (IDS) and distributed optical fiber sensor (DOFS). A three-dimensional physical model of

3.6
 
m
×
2.0
 
m
×
2.0
 
m

was built; the internal displacement of rock was measured by IDS. BOTDA distributed optical fiber was used to monitor the dynamic movement and deformation law of coated rock. Finally, the two monitoring results were compared and analyzed. The results show that: (1) the displacement curve of the fractured rock mass in the key strata shows a “stepped” increase; (2) based on the analysis of displacement continuous monitoring results, the strata behavior law of working face is obtained. The first weighting interval of 61601 working face is 90 cm, and the periodic weighting steps are 105 cm, 115 cm, 135 cm, 150 cm, 165 cm, 180 cm, 215 cm, and 240 cm; (3) the average strain of fiber is proposed. The first weighting and periodic weighting laws of the working face are represented by the average strain, which are consistent with the experimental phenomena. The first weighting in the average strain curve shows the first mutation peak, and the periodic weighting shows the periodic mutation peak change of the average strain curve.
An Adaptive EEG Classification Algorithm Based on CSSD and ELM_Kernel for Small Training Samples
Authors: Wang Li , Lan Zhi , Wang Qiang , Bai Xue , Ma Fengling
Published: December 2022

Rehabilitation technologies based on brain-computer interface (BCI) have become a promising approach for patients with dyskinesia to regain movement. In BCI experiment, there is often a necessary stage of calibration measurement before the feedback applications. To reduce the time required for initial training, it is of great importance to have a method which can learn to classify electroencephalogram (EEG) signals with a little amount of training data. In this paper, the novel combination of feature extraction and classification algorithm is proposed for classification of EEG signals with a small number of training samples. For feature extraction, the motor imagery EEG signals are pre-processed, and a relative distance criterion is defined to select the optimal combination of channels. Subsequently, common spatial subspace decomposition (CSSD) algorithm and extreme learning machine with kernel (ELM_Kernel) algorithm are used to perform the types of tasks classification of motor imagery EEG signals. Simulation results demonstrate that the proposed method produces a high average classification accuracy of 99.1% on BCI Competition III dataset IVa and 76.92% on BCI Competition IV dataset IIa outperforming state-of-the-art algorithms and obtains a good classification accuracy.
Multiobjective Model for Microgrids Integrating Electric Vehicles to Grid and Building Based on Interest Balance
Authors: Chu Xiaolin , Wang Peng , Yang Dong
Published: December 2022

Microgrids allow energy exchange among multiple interconnected microgrids for greater energy efficiency and collective economic interest. However, in some cases, the benefit of some microgrids within the network may not be uncertain. In view of the increasing development of electric vehicles (EVs), a multiobjective model is proposed to improve the performance of microgrids by integrating electric vehicles-to-grid (V2G) and vehicles-to-building (V2B) based on global and individual benefit balance. Two reference models are built to verify the validity of the proposed method, and models are formulated as mixed integer linear programming formats solved by the weighting method. A set of parameters of microgrids are adopted to model the driver behaviors (e.g., available hours of EV), energy transactions (e.g., electricity), performance factor (e.g., emission factor), distributed energy (e.g., solar panel), and energy load of five commercial buildings (e.g., hotel) located in Shanghai. Simulation results demonstrate the effectiveness of the operation decision models in the energy management of microgrids under neutral, proeconomic, proenvironmental, and proenergy weighting scenarios. The case study results specify that the proposed method can achieve operational cost, CO2 emission, and primary energy consumption reductions for each microgrid, with total benefits declining slightly.
Mechanism of GLP‐1 Receptor Agonists‐Mediated Attenuation of Palmitic Acid‐Induced Lipotoxicity in L6 Myoblasts
Authors: Kong Mo-wei , Gao Yu , Xie Yu-yu , Xing En-hong , Sun Li-xin , Ma Hui-juan , Xing Han-ying
Published: December 2022

Object. L6 cells were cultured to explore the possible mechanism underlying the improvement of insulin resistance by Liraglutide (LR). Methods. Cells were divided into 5 groups—control, high‐fat, 10 nmol/L LR + 0.6 mmol/L palmitic acid (PA) (10LR), 100 nmol/L LR + 0.6 mmol/L PA (100LR), and 1000 nmol/L LR + 0.6 mmol/L PA (1000LR). CCK‐8 method to detect cell viability, GPO‐PAP enzymatic method to detect intracellular triglyceride content, and reverse transcription quantitative real‐time polymerase chain reaction (RT‐qPCR) and western blotting methods to detect fatty acid translocase CD36 (FAT/CD36) and fatty acid binding protein 4 (FABP4) in L6 cells, glucose‐regulated protein 78 (GRP78), glucose transporter 4 (GLUT4) expression at the mRNA and protein levels, respectively, were performed. Results. We found that after PA intervention for 24 h, the cell viability decreased significantly; the cell viability of the LR group was higher than that of the high‐fat group (P < 0.01). After PA intervention, compared with those in the high‐fat group, GRP‐78, FAT/CD36, FABP4 mRNA ((4.36 ± 0.32 vs. 8.15 ± 0.35); (1.00 ± 0.04 vs. 2.46 ± 0.08); (2.88 ± 0.55 vs. 8.29 ± 0.52), P < 0.01) and protein ((3338.13 ± 333.15 vs. 4963.98 ± 277.29); (1978.85 ± 124.24 vs. 2676.07 ± 100.64); (3372.00 ± 219.84 vs. 6083.20 ± 284.70), both P < 0.01) expression decreased in the LR group. The expression levels of GLUT4 mRNA ((0.75 ± 0.04 vs. 0.34 ± 0.03), P < 0.01) and protein ((3443.71 ± 191.89 vs. 2137.79 ± 118.75), P < 0.01) increased. Conclusion. Therefore, we conclude that LR can reverse PA‐induced cell inactivation and lipid deposition, which may be related to the change in GRP‐78, FAT/CD36, FABP4, GLUT4, and other factors.
Research on Grain Food Blockchain Traceability Information Management Model Based on Master-Slave Multichain
Authors: Li Yue , Zhang Xin , Zhao Zhiyao , Xu Jiping , Jiang Zixuan , Yu Jiabin , Cui Xiaoyu
Published: December 2022

Aiming at the problems such as slow traceability efficiency, poor sharing, and the difficulty of matching the throughput of a blockchain single chain structure due to the complexity of the grain food supply chain links, the large number of participants, and the large amount of data information, this paper proposes a grain food blockchain traceability information management model based on the master-slave multichain structure by analyzing the processes and data characteristics of each link in the grain food supply chain; on this basis, the PLEW consensus algorithm based on Raft + improved PoW algorithm is designed for the master chain, and the CI-PBFT consensus algorithm based on trusted information degree is designed for the slave chain. The master chain and slave chain are anchored to each other through hash locking, and the data is uploaded and queried through smart contracts. In order to verify the effectiveness of the model, the blockchain traceability system is designed and implemented based on Hyperledger Fabric2.2. At the same time, it is compared with the transaction throughput and traceability efficiency of the blockchain single chain structure. Through the safety analysis of the data information of a company in Hubei, the results show that the grain traceability system designed and implemented in this study has certain advantages over the blockchain single chain structure in all aspects. It can also solve the grain food security problems that consumers worry about, and provide reference for the research of grain blockchain traceability information management.
Novel Technique for Hand-Assisted Laparoscopic Nephrectomy for Advanced Renal Cell Carcinoma with Renal Vein and Inferior Vena Cava Thrombi: Three Case Reports
Authors: Machida Yuichi , Yoshiuchi Hiroki , Kitano Yuko , Kuroki Yoshikazu , Kamizuru Masato , Uchida Junji
Published: December 2022

Introduction. The treatment of thrombi in the renal vein (RV) and inferior vena cava (IVC) requires advanced laparoscopic experience. We present three cases of hand-assisted laparoscopic nephrectomy (HALN) using a novel technique for treating advanced renal cell carcinoma (RCC) with thrombi in the RV and IVC. Case Presentation. Three patients with RCC with RV or IVC thrombus below level I underwent HALN. Two patients had right RCC with RV and IVC thrombi. One patient had left RCC with an RV thrombus. We hooked a vessel loop to the end of the thrombus and pulled it up manually to make space for vascular processing. The RV was narrowed and dissected using Hem-o-lok clips or an Endo GIA stapler. Conclusion. In carefully selected cases, renal vascular processing could be easily and safely performed using a vessel loop in HALN with thrombectomy.
Validation of Antidiabetic and Antihyperlipidemic Effects of 80% Methanolic Extract of the Lonchocarpus laxiflorus Leaves in Streptozotocin-Induced Diabetic Swiss Albino Mice
Authors: Desta Getaye Tessema , Ferede Yared Andargie , Zewdu Woretaw Sisay , Adugna Baye Yrga , Arega Tilaye , Alemu Muluken Adela
Published: December 2022

Introduction. Diabetes mellitus (DM) is a chronic endocrine disorder that requires long-term treatment. In Ethiopian traditional medicine practice, plants have been provided with a vital role in fighting human and animal diseases since ancient times. The aqueous extract of Lonchocarpus laxiflorus (L. laxiflorus) leaves has been consumed for treating diabetes mellitus without confirming its safety and efficacy scientifically. This experiment aimed to evaluate the safety and antidiabetic efficacy of the leaf extract of L. laxiflorus in mice models. Methods. The crude extraction was conducted using a cold maceration technique and 80% methanol solvent. Normoglycemic, oral glucose-loaded, and streptozotocin-induced (STZ) diabetic models were employed. Male Swiss albino mice were randomly grouped into five categories( with six mice per group during normoglycemic, oral glucose-loadingtest as a negative control, positive control, and three treatment groups. In STZ-induced diabetic models, the groups include normal and diabetic negative control, diabetic positive control, and three diabetic treatment groups. The negative control groups received vehicles, the positive control received 5 mg/kg glibenclamide, and the treatment groups received the crude extract at 100, 200, and 400 mg/kg doses, respectively. Results. Up to 2000 mg/kg crude extract, neither signs of toxicity nor death were observed. In normoglycemic mice, there was a significant blood glucose reduction at 200 and 400 mg/kg doses starting from the 2nd h post-administration. The oral glucose load showed a significant antihyperglycemic effect at 200 and 400 mg/kg of the crude extract and glibenclamide. In STZ-induced diabetic models, the 200, 400 mg/kg crude extract, and glibenclamide showed a significant antidiabetic activity and enhancement of a good serum lipid profile. Conclusion. This study confirmed that the leaf of L. laxiflorus was safe and possesses antidiabetic and antidyslipidemic activities.
Reconstruction Layout Optimization of Multivariety and Small Batch Workshop in Aerospace Industry
Authors: Zhao Baohai , Jiang Xingyu , Tian Zhiqiang , Chen Keqiang , Yang Guozhe , Liu Shun , Wang Minghao , Liu Weijun
Published: December 2022

To cope with the problems of frequent mold changes, long production cycles and serious logistics crossings in workshop of aerospace enterprise. First, a manufacturing cell layout planning method based on the feature bit code domain method and K-Means++ is proposed to realize the accurate division of manufacturing cells. Then, a multiobjective optimization method of dynamic reconstruction layout based on improved fruit fly optimization algorithm (IFOA) is proposed to solve the reconstruction layout optimization of the production workshop problem with the optimization objectives of logistics cost, reconstruction cost, loss cost, and cell integrated area. Finally, plant simulation software is applied to simulate the workshop layout before and after optimization. The simulation results show that the logistics cost of the workshop cell layout after optimization is reduced by 8.7%, the utilization rate of the workshop area is improved by 5.2%, and the value-added rate of products is increased by 6.6%, which verifies the effectiveness and feasibility of the proposed model and method.
Influence of Maleinized Polybutadiene on Adhesive Strength and Toughness of Epoxy Resins
Authors: Panthakkal Abdul Muthalif Mohammed , Choe Youngson
Published: December 2022

This study explored the effect of maleinized polybutadiene (MPB) on the mechanical properties of epoxy resins. Diglycidyl ether of bisphenol-A, an epoxy resin, was modified by incorporating MPB having different molecular weights in order to improve the fracture toughness and peel strength. MPB was mixed with epoxy resin at several concentrations (5, 10, and 15 phr), with the epoxy resin as the major phase and MPB as the minor phase. A comparative study was performed to investigate the influence of MPB on epoxy resins based on their molecular weight difference. Lap shear test results showed that the shear strength of the MPB-modified epoxy resins was superior to that of the neat epoxy resin. At 10 wt% MPB loading, the modified epoxy resin exhibited an 87% enhancement in T-peel strength relative to that of the neat epoxy resin. Moreover, the fracture energy of the modified epoxy system increased proportionally with the amount of MPB in the epoxy matrix. These results indicate that MPB incorporation is a simple and effective method for designing multifunctional epoxy resins, thus facilitating their industrial application in various spheres.
Two Cases of Hemophagocytic Lymphohistiocytosis Associated with Disseminated Histoplasmosis Presented with Transient Pancytopenia
Authors: Apriany Novi , Sukorini Usi , Ratnaningsih Tri , Asdie Rizka Humardewayanti , Subronto Yanri Wijayanti , Hutajulu Susanna Hilda , Purwanto Ibnu , Hardianti Mardiah Suci
Published: December 2022

Transient pancytopenia due to reactive bone marrow suppression often occurs in hemophagocytic lymphohistiocytosis (HLH), a syndrome resulting from excessive immune activation following a severe infection. We reported two cases with pancytopenia and disseminated histoplasmosis accompanied by HLH, initially suspected to be blood malignancies. Our first case documented the relevance between the improvement of pancytopenia and the clearance of Histoplasma capsulatum in serial bone marrow aspirations. The second case showed immense Histoplasma engulfment by the macrophage in relation to a severe clinical condition, followed by improvement of clinical symptoms in accordance with the recovery of pancytopenia. These two cases highlighted the importance of comprehensive and critical analysis for cases with concurrent pancytopenia and severe infection, since it may be that the pancytopenia underlies the severe infection or vice versa.
Crecimiento
Authors: González Pablo
Published: December 2022

Los datos del tercer trimestre confirman, en general, que la economía continúa su proceso de ajuste. El PIB no minero retrocedió 0,8% respecto del trimestre anterior en su serie desestacionalizada, su segunda caída consecutiva… Por el lado de la demanda, la formación bruta de capital fijo (FBCF) tuvo un desempeño significativamente mejor al previsto en el tercer trimestre… Las perspectivas para la inversión siguen siendo débiles. Continuar leyendo...
Obstáculos para innovar
Authors: Ortiz Rodrigo
Published: December 2022

La innovación empresarial es fundamental para mejorar la productividad e impulsar el desarrollo económico (Fagerberg et al., 2010). Sin embargo, las innovaciones pueden verse seriamente obstaculizadas por las percepciones de las empresas sobre la complejidad técnica y no técnica involucrada en tales actividades de innovación. Así, los obstáculos para innovar suelen definirse como los impedimentos que enfrentan las empresas al desarrollar actividades de innovación (Piatier, 1984). Continuar leyendo...
Identification of Biomarkers Associated with the Prognoses of Colorectal Cancer Patients
Authors: Fang Yuejun , Zhan Xiaoan
Published: December 2022

Introduction: Colorectal cancer (CRC) is a common cancer. As metastasis and recurrence are main causes of CRC death, it is of great significance to find prognostic biomarkers. Methods: Data related to CRC were collected from GEO database. The patients were grouped based on clinical information, and the differentially expressed genes (DEGs) were obtained by differential analysis. GO and KEGG pathway enrichment analyses were conducted based on DEGs. Cox combined with LASSO regression analysis was applied to screen out the key genes that used to build the prognostic model. Survival curve and receiver operating characteristic curve were employed to evaluate the validity and reliability of the model. Cox regression analysis was applied to determine the independence of risk score. GSEA and GSVA analyses were performed on patients with different risks according to the risk model scores, and the prognostic nomogram was plotted combined with clinical data. Also, qRT-PCR was applied to examine the expression status of the screened signatures in clinical cases. Results: We obtained 302 DEGs by dividing CRC patients into early-stage and advanced-stage groups. The results of enrichment analyses demonstrated that the DEGs were mainly concentrated in tissues of extracellular matrix, epithelial cell proliferation, and cell adhesion-related pathways. Regression identified 9 hub genes notably correlated with prognosis, including CLK1, SLC2A3, LIPG, EPHB2, ATOH1, PLCB4, GZMB, CKMT2, and CXCL11. The validation of the risk model proved that the risk model was accurate and could independently determine the prognosis of patients. Finally, differences were found in pathway activity of extracellular matrix secretion, plaque secretion, Notch signaling pathway, and tight junctions in high-risk and low-risk patients. In addition to LIPG and CKMT2, other feature genes were notably overexpressed in CRC tumor tissues. Conclusion: The results proved that the expression levels of the 9 biomarkers could be used to predict the prognosis of CRC patients.
“Mauricius von Craûn”
Authors: Schneidergruber Dietmar
Published: December 2022

To this day, “Mauricius of Craûn” still holds a number of research desiderata. Author and dating are undetermined, but also the purpose of this “Märe” is to be questioned. Records appeared late and thus provide little information for the research questions. The author’s approach to this “Märe” is to look at the characters as allegorical figures. This results in a new perspective and it also indicates the text’s closeness to the papal bull “Vox in Rama” in terms of language. Pachurka has already demonstrated the relationship between a Latin work of Heinrich von Avranches and Walther von der Vogelweide in 2020. Here, too, the linguistic proximity is obvious and quite deliberately chosen. This essay is intended as an impulse for thought, whereby the author can actually only serve as a likely thesis. However, the “Märe” “Mauricius of Craûn” would fit into the lifetime achievements of Heinrich von Avranches.
Frege’s Epistemic Criterion of Thought Individuation
Authors: Hawkins Nathan
Published: December 2022

Frege believes that the content of declarative sentences divides into a thought and its ‘colouring’, perhaps combined with assertoric force. He further thinks it is important to separate the thought from its colouring. To do this, a criterion which determines sameness of sense between sentences must be deployed. But Frege provides three criteria for this task, each of which adjudicate on different grounds. In this article, rather than expand on criticisms levelled at two of the criteria offered, the author focuses on the most promising candidate. As it stands, this criterion has problems, but not insuperable ones. He suggests an adjusted criterion that relies on the epistemic notion of triviality. He recommends this criterion as both harmonious with Frege’s broader thought and preferable to alternatives offered. The moral is that Frege individuates thoughts by deploying an epistemic concept, and this is the only suitable way for him to do so.
Reflections on Krste Misirkov’s Theory: From Ethnocultural Entity to Politically Legitimate Nation
Authors: Cvetanova Ganka
Published: December 2022

Reflections on Krste Misirkov’s Theory: From Ethnocultural Entity to Politically Legitimate Nation In order to present some of the aspects that created the preconditions for the Macedonian nation-building process and its political legitimacy, this paper reviews the Macedonian question through the works of Krste P. Misirkov and his struggle for the separation and recognition of Macedonian ethnocultural entity as a distinct one. His conceptions are analysed through the lens of modernist theories of nation and nationalism, with special emphasis on the following aspects: nation as a historically and socially formed construct, a sense of common belonging, high cultures and standardized languages as the political legitimacy of a nation, the role of cultural elites in the nation-building process, and the relationship between the nation and the modern state.Refleksje nad teorią Krste Misirkova. Od jednostki etnokulturowej do uprawnionego politycznie naroduW niniejszym artykule została podjęta kwestia macedońska przez pryzmat prac Krste P. Misirkova i jego walki o wyodrębnienie i uznanie macedońskiego bytu etnokulturowego. Celem jest przedstawienie wybranych czynników, które stworzyły warunki wstępne do procesu budowania narodu macedońskiego i jego politycznej legitymizacji. Koncepcje Misirkova analizowane są w świetle modernistycznych teorii narodu i nacjonalizmu, ze szczególnym uwzględnieniem następujących aspektów: naród jako ukształtowany historycznie i społecznie konstrukt, poczucie wspólnej przynależności, kultura wysoka i ustandaryzowane języki jako polityczna legitymizacja narodu, rola elit kulturalnych w procesie tworzenia narodu oraz relacje między narodem a nowoczesnym państwem.
Panoptic Vision of the World: Can a State of Emergency Become a Regular One?
Authors: Kulavkova Katica
Published: December 2022

Panoptic Vision of the World: Can a State of Emergency Become a Regular One?The object of interpretation of this text is several social aspects of the Coronavirus SARS-CoV-2 pandemic which have equivocal and contradictory meanings: state of emergency/crisis, emergency measures, civil and human rights/restrictions to human rights, freedom/limitation of freedom. The basic interpretative and conceptual tools used are the terms ‘panopticon’ and ‘panopticism’, whose archetypal patterns point to systematic and systemic damage to the universal human rights to freedom and privacy. This damage occurs by legalizing the surveillance and control of citizens, thus becoming more akin to radical surveillance. The pandemic is seen as an excuse to renew the panoptic vision of the world. The contemporary pandemic surveillance of citizens dissolves the boundaries between the real and the virtual and creates new boundaries of freedom on several levels: movement, speech, work, communication, existence. Some of these limitations of human rights and freedoms relate to the elderly population. This analysis shows the danger of prolonging and legalizing emergency measures in circumstances when, realistically, there is no state of emergency. This poses a question: can a state of emergency become a regular state? The New Normal has the power to create alienated individuals and an alienated society.Panoptyczna wizja świata. Czy stan wyjątkowy może stać się normalnym?Przedmiotem uwagi jest kilka aspektów społecznych pandemii koronawirusa SARS-CoV-2, które mają niejednoznaczne i sprzeczne znaczenia: stan wyjątkowy / kryzys, środki nadzwyczajne, prawa obywatelskie i prawa / ograniczenia praw człowieka, wolność / ograniczenie wolności. Podstawowymi narzędziami interpretacyjnymi i pojęciowymi są terminy „panoptykon” i „panoptycyzm”, których archetypowe wzorce wskazują na systematyczne i systemowe niszczenie uniwersalnych praw człowieka do wolności i prywatności. Ta szkoda pojawia się poprzez legalizację nadzoru i kontroli obywateli, przez co staje się bardziej zbliżona do radykalnej inwigilacji. Pandemia jest postrzegana jako pretekst do odnowienia panoptycznej wizji świata. Współczesny pandemiczny nadzór nad obywatelami zaciera granice między tym, co realne, a tym, co wirtualne i tworzy nowe granice wolności na kilku poziomach: ruchu, mowy, pracy, komunikacji, egzystencji. Niektóre z tych ograniczeń praw i wolności człowieka dotyczą osób starszych. Analiza pokazuje niebezpieczeństwo przedłużenia i legalizacji środków nadzwyczajnych w sytuacji, gdy realnie nie ma stanu wyjątkowego. Powstaje pytanie: czy stan wyjątkowy może stać się stanem normalnym? New Normal ma moc tworzenia wyalienowanych jednostek i wyalienowanego społeczeństwa.