bluuebunny/crossref_metadata_2025 huggingface dataset:
Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
Authors: Li Shengmei , Geng Shiwei , Pang Bo , Zhao Jieyin , Huang Yajie , Rui Cun , Cui Jinxin , Jiao Yang , Zhang Ru , Gao Wenwei
Published: May 2022

Fiber length is an important indicator of cotton fiber quality, and the time and rate of cotton fiber cell elongation are key factors in determining the fiber length of mature cotton. To gain insight into the differences in fiber elongation mechanisms in the offspring of backcross populations of Sea Island cotton Xinhai 16 and land cotton Line 9, we selected two groups with significant differences in fiber length (long-fiber group L and short-fiber group S) at different fiber development stages 0, 5, 10 and 15 days post-anthesis (DPA) for transcriptome comparison. A total of 171.74 Gb of clean data was obtained by RNA-seq, and eight genes were randomly selected for qPCR validation. Data analysis identified 6055 differentially expressed genes (DEGs) between two groups of fibers, L and S, in four developmental periods, and gene ontology (GO) term analysis revealed that these DEGs were associated mainly with microtubule driving, reactive oxygen species, plant cell wall biosynthesis, and glycosyl compound hydrolase activity. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that plant hormone signaling, mitogen-activated protein kinase (MAPK) signaling, and starch and sucrose metabolism pathways were associated with fiber elongation. Subsequently, a sustained upregulation expression pattern, profile 19, was identified and analyzed using short time-series expression miner (STEM). An analysis of the weighted gene coexpression network module uncovered 21 genes closely related to fiber development, mainly involved in functions such as cell wall relaxation, microtubule formation, and cytoskeletal structure of the cell wall. This study helps to enhance the understanding of the Sea Island–Upland backcross population and identifies key genes for cotton fiber development, and these findings will provide a basis for future research on the molecular mechanisms of fiber length formation in cotton populations.
Excess Mortality on Italian Small Islands during the SARS-CoV-2 Pandemic: An Ecological Study
Authors: Riccò Matteo , Ferraro Pietro , Peruzzi Simona , Zaniboni Alessandro , Satta Elia , Ranzieri Silvia
Published: May 2022

Small islands have been considered at an advantage when dealing with infectious diseases, including COVID-19, but the evidence is still lacking. Crude mortality rates (CMRs) and excess mortality rates (EMRs) were calculated for 35 municipalities on the Italian small islands for 2020 and 2021, and the corresponding estimates were compared to those of the parent provinces and the national estimates. Notification rates for COVID-19 were retrieved, but detailed data at the municipality level were not available. A relatively low CMR (1.069 per 100 per year, 95% confidence interval [95% CI] 0.983–1.164) was identified in 2020, compared to 1.180, 95% CI 1.098–1.269 for 2021. EMRs of small islands ranged between −25.6% and +15.6% in 2020, and between −13.0% and +20.9% in 2021, with an average gain of +0.3% (95% CI −5.3 to +5.8) for the entirety of the assessed timeframe, and no substantial differences between 2020 and 2021 (pooled estimates of −4.1%, 95% CI −12.3 to 4.1 vs. 4.6%, 95% CI −3.1 to 12.4; p = 0.143). When dealing with COVID-19 notification rates, during the first wave, parent provinces of Italian small islands exhibited substantially lower estimates than those at the national level. Even though subsequent stages of the pandemic (i.e., second, third, and fourth waves) saw a drastic increase in the number of confirmed cases and CMR, estimates from small islands remained generally lower than those from parent provinces and the national level. In regression analysis, notification rates and mortality in the parent provinces were the main effectors of EMRs in the small islands (β = 0.469 and β = 22.768, p < 0.001 and p = 0.007, respectively). Contrarily, the management of incident cases in hospital infrastructures and ICUs was characterized as a negative predictor for EMR (β = −11.208, p = 0.008, and −59.700, p = 0.003, respectively). In summary, the study suggests a potential role of small geographical and population size in strengthening the effect of restrictive measures toward countering the spread and mortality rate of COVID-19.
Correlation between the Photographic Cranial Angles and Radiographic Cervical Spine Alignment
Authors: Kawasaki Tomoko , Ohji Shunsuke , Aizawa Junya , Sakai Tomoko , Hirohata Kenji , Kuruma Hironobu , Koseki Hirohisa , Okawa Atsushi , Jinno Tetsuya
Published: May 2022

The cranial vertical angle (CVA) and cranial rotation angle (CRA) are used in clinical settings because they can be measured on lateral photographs of the head and neck. We aimed to clarify the relationship between CVA and CRA photographic measurements and radiographic cervical spine alignment. Twenty-six healthy volunteers were recruited for this study. Lateral photographs and cervical spine radiographs were obtained in the sitting position. The CVA and CRA were measured using lateral photographs of the head and neck. The C2-7 sagittal vertical axis (SVA), cervical lordosis (C2-7), and occipito-C2 lordosis (O-C2) were measured using radiographic imaging as a standard method of evaluating cervical spine alignment. Correlations between the CVA and CRA on photographs and cervical spine alignment on radiographs were analyzed. The CVA and SVA were significantly negatively correlated (ρ = −0.51; p < 0.05). Significant positive correlations were found between CVA and C2-7 (ρ = 0.59; p < 0.01) and between CRA and O-C2 (ρ = 0.65; p < 0.01). Evaluating the CVA and CRA on photographs may be useful for ascertaining head and neck alignment in the mid-lower and upper parts of the sagittal plane.
Monitoring Eye Movements Depending on the Type of Visual Stimulus in Patients with Impaired Consciousness Due to Brain Damage
Authors: Kujawa Katarzyna , Żurek Alina , Gorączko Agata , Olejniczak Roman , Zurek Grzegorz
Published: May 2022

The eyeballs are often the only way to communicate messages as a result of brain damage. However, it is not uncommon for them to become dysfunctional, thus requiring the introduction of appropriate therapy. The trajectory of eye movements (saccadic movements and gaze fixation) during observation of a static and dynamic point presented with an eye tracker was analyzed in the present study. Twelve patients with brain injury of different etiology, with different degrees of consciousness disorders and not communicating through verbal and motor skills, qualified for the study. All participants demonstrated greater eye movement activity when presented with a dynamic task in which they observed a moving point. The findings suggest that effective eye movement therapy must incorporate dynamic stimuli.
Analysis of Motives and Factors Connected to Suicidal Behavior in Patients Hospitalized in a Psychiatric Department
Authors: Bednarova Aneta , Hlavacova Natasa , Pecenak Jan
Published: May 2022

(1) Background: This study aimed to investigate the motives and factors connected to suicidal behavior in 121 hospitalized patients with intentional self-harm (diagnosis X 60-81 according to the ICD-10); (2) Methods: Suicidal behavior of the patient was assessed from data obtained by psychiatric examinations and by the Columbia Suicide Severity Rating Scale. Analysis of data to identify the patients’ reason and motives behind suicidal behavior in a group of patients with a suicide attempt (SA, n = 80) and patients with Non-Suicidal Self-Injurious Behavior (NSSIB, n = 41) was carried out; (3) Results: Results showed that patients with affective disorder have a 19-times higher rate of SA against other diagnoses. Patients with personality disorders have a 32-times higher rate of NSSIB than patients with other diagnoses. Living alone and the absence of social support increased the likelihood of SA. Qualitative data analysis of patients’ statements showed different themes in the justification of motives for suicidal behavior between SA and NSSIB cases. Significant differences were shown for non-communicated reasons, loneliness, social problems, extortion, and distress; (4) Conclusions: The evaluation of patients’ verbal statements by qualitative analysis during the psychiatric examination should be considered in clinical practice. It should be considered to include self-poisoning in the criteria of the Non-suicidal Self-Injury diagnostic categories.
Defining Household Typologies Based on Cropland Use Behaviors for Rural Human-Environment Systems Simulation Research: A Case Study in Southwest China
Authors: Li Ming , Wang Yukuan , Tian Congshan , Yang Liang Emlyn , Hossain Md. Sarwar
Published: May 2022

The dynamics of rural human-environment systems in developing countries have increasingly been attracting attention. Agent-based modeling (ABM) is a valuable simulation tool for detecting complex feedback loops in rural human-environment systems with a ‘bottom-up’ approach. However, such models require the prerequisite analysis of household typology to simulate households’ decision-making process, where a gap exists between having accurate classification criteria and a simplified modeling framework. This study aimed to develop a household typology for two selected counties in southwest China based on multivariate analysis techniques and the classification tree method. Four categories of socioeconomic variables, including labor conditions, resource endowments, economic status, and social connections, were screened as possible factors impacting agriculture practice decisions. The results showed that household diversification in the study area was mainly determined by diversified livelihood strategies of off-farm work, livestock breeding, subsidy dependence, and traditional planting. Five distinct household types were identified: non-farm households, part-time households, livestock breed households, subsidized households, and traditional planting households. The household types were associated with specific cropland use behaviors, and their decision-making behaviors were verified with bounded rationality theory (where the maximization of profits is the primary goal). The quantitative classification criteria obtained in this study were clear and could be easily identified and used by ABMs. Our study provides a basis for further simulation of the complicated rural human-environment systems in southwest China.
Health Status, Health-Related Factors and Work Environment in Korean Semiconductor Workers between 1984–2012: A Qualitative Study and a Cross-Sectional Study
Authors: Kim Kyungsik , Sung Ho Kyung , Jang Jieun , Suh Eunyoung E. , Lee Kwan , Park Sue K.
Published: May 2022

Background: The environment of semiconductor facilities and exposure status has undergone changes. To identify changes in the work environment, health status, and risk factors, a qualitative and cross-sectional study was conducted. Methods: For the qualitative study, 38 current and retired workers who worked for at least 10-years were studied; for the cross-sectional study, 306 current workers who worked for at least 5-years based on JEM strata from Samsung Electronics were selected. Participants were asked about occupational history, cancer-relating risk factors during the working period, medical history, dietary habits for the past year, and hematological cancer-relating infection. Results: In the qualitative study, fabrication workers reported bladder cystitis, dermatitis in hands, gastritis/ulcer, and dry eye as frequent symptoms during work environment changes (manual to automatic process). In the cross-sectional study, there were no abnormal findings for infection biomarkers related to hematological cancer and spontaneous abortion, and their general health status was no different from the general population. Also, questionnaire feasibility was evaluated for the applicability in the prospective cohort. Conclusion: Current semiconductor workers’ health status was good compared to other populations. For lifelong health assessment, a cohort study is needed which considers health worker effects and current environments.
Effect of Working from Home on the Association between Job Demands and Psychological Distress
Authors: Eguchi Hisashi , Inoue Akiomi , Hino Ayako , Tsuji Mayumi , Tateishi Seiichiro , Ikegami Kazunori , Nagata Tomohisa , Matsugaki Ryutaro , Fujino Yoshihisa ,   on behalf of the CORoNaWork Project
Published: May 2022

Limited information is available regarding the association between workplace psychosocial factors and general mental health status among workers during the coronavirus disease 2019 pandemic. This study examined how working from home affected the association between job demands and psychological distress (PD). A cross-sectional online survey was conducted in December 2020 (N = 27,036). The dependent variable (PD) was assessed using the Kessler Psychological Distress Scale. Job demands were assessed using the Job Content Questionnaire. Working from home was determined by participants’ responses to the question, “Do you currently work from home?” We used a two-level regression analysis adjusted for prefecture. Each individual-level variable at Level 1 was nested into each prefecture at Level 2, stratified by working from home or not. Overall, 21.3% of participants worked from home. The interaction between working from home and job demands was significant. Job demands were positively associated with PD. The stratified analysis indicated that the associations were weaker among employees who worked from home compared with those among employees who did not. The association between job demands and PD may be weakened by working from home.
Mercury Pollution from Artisanal and Small-Scale Gold Mining in Myanmar and Other Southeast Asian Countries
Authors: Soe Pyae Sone , Kyaw Win Thiri , Arizono Koji , Ishibashi Yasuhiro , Agusa Tetsuro
Published: May 2022

Mercury (Hg) is one of the most harmful metals and has been a public health concern according to the World Health Organization (WHO). Artisanal and small-scale gold mining (ASGM) is the world’s fastest-growing source of Hg and can release Hg into the atmosphere, hydrosphere, and geosphere. Hg has been widely used in ASGM industries throughout Southeast Asia countries, including Cambodia, Indonesia, Laos, Malaysia, Myanmar, the Philippines, and Thailand. Here, 16 relevant studies were systematically searched by performing the PRISMA flow, combining the keywords of “Hg”, “ASGM”, and relevant study areas. Mercury concentrations exceeding the WHO and United States Environmental Protection Agency guideline values were reported in environmental (i.e., air, water, and soil) and biomonitoring samples (i.e., plants, fish, and human hair). ASGM-related health risks to miners and nonminers, specifically in Indonesia, the Philippines, and Myanmar, were also assessed. The findings indicated severe Hg contamination around the ASGM process, specifically the gold-amalgamation stage, was significantly high. To one point, Hg atmospheric concentrations from all observed studies was shown to be extremely high in the vicinity of gold operating areas. Attentions should be given regarding the public health concern, specifically for the vulnerable groups such as adults, pregnant women, and children who live near the ASGM activity. This review summarizes the effects of Hg in Myanmar and other Southeast Asian countries. In the future, more research and assessment will be required to investigate the current and evolving situation in ASGM communities.
Spatio-Temporal Characteristics of PM2.5 Concentrations in China Based on Multiple Sources of Data and LUR-GBM during 2016–2021
Authors: Dai Hongbin , Huang Guangqiu , Wang Jingjing , Zeng Huibin , Zhou Fangyu
Published: May 2022

Fine particulate matter (PM2.5) has a continuing impact on the environment, climate change and human health. In order to improve the accuracy of PM2.5 estimation and obtain a continuous spatial distribution of PM2.5 concentration, this paper proposes a LUR-GBM model based on land-use regression (LUR), the Kriging method and LightGBM (light gradient boosting machine). Firstly, this study modelled the spatial distribution of PM2.5 in the Chinese region by obtaining PM2.5 concentration data from monitoring stations in the Chinese study region and established a PM2.5 mass concentration estimation method based on the LUR-GBM model by combining data on land use type, meteorology, topography, vegetation index, population density, traffic and pollution sources. Secondly, the performance of the LUR-GBM model was evaluated by a ten-fold cross-validation method based on samples, stations and time. Finally, the results of the model proposed in this paper are compared with those of the back propagation neural network (BPNN), deep neural network (DNN), random forest (RF), XGBoost and LightGBM models. The results show that the prediction accuracy of the LUR-GBM model is better than other models, with the R2 of the model reaching 0.964 (spring), 0.91 (summer), 0.967 (autumn), 0.98 (winter) and 0.976 (average for 2016–2021) for each season and annual average, respectively. It can be seen that the LUR-GBM model has good applicability in simulating the spatial distribution of PM2.5 concentrations in China. The spatial distribution of PM2.5 concentrations in the Chinese region shows a clear characteristic of high in the east and low in the west, and the spatial distribution is strongly influenced by topographical factors. The seasonal variation in mean concentration values is marked by low summer and high winter values. The results of this study can provide a scientific basis for the prevention and control of regional PM2.5 pollution in China and can also provide new ideas for the acquisition of data on the spatial distribution of PM2.5 concentrations within cities.
Elderly Caregiving Quality Improvement: A Pilot Study of the Burdens of Vietnamese Caregivers in Taiwan
Authors: Wu Chang-Yu , Li Yu-Ying , Lyver Maurice J.
Published: May 2022

Taiwan is expected to reach super-aged status by 2026, leading to an increased demand for elderly caregiving services. Low local unemployment and a dwindling working-age population mean the island’s care system relies heavily on female foreign domestic workers (FDWs) from Southeast Asian neighbors such as Vietnam to satisfy labor shortages. Although suggested by anecdotal evidence, limited research has been conducted on the link between the shortfall in FDW qualifications, training, preparedness, and expertise and their employment stressors. Therefore, this study aims to assist FDWs by evaluating their stressors and helping them better understand health care delivery by (1) administering the Modified Caregiver Strain Index (MCSI) revised 2003 questionnaire, (2) performing semi-structured in-depth one-on-one interviews, (3) classifying interview results according to thematic analysis, and (4) using these themes to devise and deliver a 12-week multilingual health education teach-back program. Our results indicate that Vietnamese FDWs face specific challenges, including language barriers, homesickness, intensive physical and psychological work demands, stress adaptation, and occupational exposures. Despite yielding no significant improvements in caregiving strain, our intervention, conducted at the height of the COVID-19 pandemic, pinpoints and classifies areas of grave concern and proposes recommendations that can assist long-term care (LTC) stakeholders in understanding and overcoming their respective challenges, thereby improving the quality of elderly care.
Acute, Sublethal, and Developmental Toxicity of Kratom (Mitragyna speciosa Korth.) Leaf Preparations on Caenorhabditis elegans as an Invertebrate Model for Human Exposure
Authors: Hughes Samantha , van de Klashorst David , Veltri Charles A. , Grundmann Oliver
Published: May 2022

Kratom (Mitragyna speciosa Korth.) is a tree native to Southeast Asia with stimulant and opioid-like effects which has seen increased use in Europe and North America in recent years. Its safety and pharmacological effects remain under investigation, especially in regard to developmental and generational toxicity. In the current study, we investigated commercial kratom preparations using the nematode Caenorhabditis elegans as a translational model for toxicity and pharmacological effects. The pure alkaloids mitragynine and 7-hydroxymitragynine as well as aqueous, ethanolic, and methanolic extracts of three commercial kratom products were evaluated using a battery of developmental, genotoxic, and opioid-related experiments. As determined previously, the mitragynine and 7-hydroxymitragynine content in kratom samples was higher in the alcoholic extracts than the aqueous extracts. Above the human consumption range equivalent of 15–70 µg/mL, kratom dose-dependently reduced brood size and health of parent worms and their progeny. 7-hydroxymitragynine, but not mitragynine, presented with toxic and developmental effects at very high concentrations, while the positive control, morphine, displayed toxic effects at 0.5 mM. Kratom and its alkaloids did not affect pumping rate or interpump interval in the same way as morphine, suggesting that kratom is unlikely to act primarily via the opioid-signalling pathway. Only at very high doses did kratom cause developmental and genotoxic effects in nematodes, indicating its relative safety.
Reply to Viner, A.; Ayrey, S. Comment on “Scheepers et al. Comparative Performance Testing of Respirator versus Surgical Mask Using a Water Droplet Spray Model. Int. J. Environ. Res. Public Health 2021, 18, 1599”
Authors: Scheepers Paul T. J. , Wertheim Heiman F. L. , van Dael Maurice , Anzion Rob , Holterman Henk Jan , Teerenstra Steven , de Groot Martijn , Voss Andreas , Hopman Joost
Published: May 2022

The response to our publication by Andrew Viner and Stewart Ayrey is much appreciated [...]
Spatial Differences and Influential Factors of Urban Carbon Emissions in China under the Target of Carbon Neutrality
Authors: Liu Kai , Ni Ziyi , Ren Mei , Zhang Xiaoqing
Published: May 2022

Cities are areas featuring a concentrated population and economy and are major sources of carbon emissions (CEs). The spatial differences and influential factors of urban carbon emissions (UCEs) need to be examined to reduce CEs and achieve the target of carbon neutrality. This paper selected 264 cities at the prefecture level in China from 2008 to 2018 as research objects. Their UCEs were calculated by the CE coefficient, and the spatial differences in them were analyzed using exploratory spatial data analysis (ESDA). The influential factors of UCEs were studied with Geodetector. The results are as follows: (1) The UCEs were increasing gradually. Cities with the highest CEs over the study period were located in the urban agglomerations of Beijing–Tianjin–Hebei, Yangtze River Delta, Pearl River Delta, middle reaches of the Yangtze River, and Chengdu–Chongqing. (2) The UCEs exhibited certain global and local spatial autocorrelations. (3) The industrial structure was the dominant factor influencing UCEs.
Child Disruptions, Remote Learning, and Parent Mental Health during the COVID-19 Pandemic
Authors: Deeb Stephanie , Madden Devin , Ghebretinsae Timnit , Lin Joyce , Ozbek Umut , Mayer Victoria , Vangeepuram Nita
Published: May 2022

New York City (NYC) was an epicenter of the COVID-19 pandemic, which resulted in broad economic, social, and emotional consequences in the lives of individuals. The current study examined associations between pandemic-related stressors and adverse mental health symptoms among NYC parents/caregivers. Community-based participatory research was used to develop a survey, and logistic regression models were utilized to assess associations between factors including disruptions in child routines and remote learning, and parent-reported symptoms of stress, anxiety, depression, and post-traumatic stress disorder (PTSD). Some 91.0% of parents reported stress and 41.2, 26.6, and 33.7% reported symptoms of anxiety, depression, and PTSD, respectively. Most parents (87.6%) reported cancellation of at least one child activity. Of the parents, 60.3% reported that their children participated in remote learning and the majority (70.3%) reported feeling overwhelmed by it. Having more cancelled child activities was associated with higher odds of reported mental health symptoms, with not being able to play outside associated with higher odds of anxiety (1.80 (1.26, 2.58), p = 0.001), depression (1.93 (1.29, 2.91), p = 0.002), PTSD (1.64 (1.13, 2.39), p = 0.009), and stress (2.34 (1.27, 4.44), p = 0.008). Feeling overwhelmed by remote learning was also associated with higher odds of all four outcomes. Pre-existing mental illness, lower resilience scores, and lower socioeconomic status emerged as additional factors associated with symptoms of mental illness. These findings highlight the importance of resources to minimize adverse psychological effects among vulnerable families.
Health Help-Seeking Behavior: The Scavengers’ Perspective
Authors: Malik Beenish , Lyndon Novel , Chin Yew Wong , Hussain Basharat , Malik Sehrish
Published: May 2022

Scavengers are prone to various health problems, hence seeking healthcare is inevitable. Despite the importance of the help-seeking behavior of scavengers, it has not been addressed explicitly in the existing literature. Addressing this gap, this study intends to explore the help-seeking behavior of scavengers and to identify the factors that influence healthcare utilization among them. This qualitative study conducted thirty-one in-depth interviews through a semi-structured interview guide and analyzed them using thematic analysis. Andersen’s Behavioral Model of Health Service Use was employed to explore the findings. The findings showed that the scavengers utilized multiple healthcare options depending on the severity and reoccurrence of the illness. The process of help-seeking and health services utilization was largely influenced by the cost of the health service, long distance to the health facility, traveling cost and waiting time. The study highlights the need for scavengers’ enrolment in micro health insurance schemes. The initiative would facilitate scavengers’ access to medical care. Health awareness campaigns and the provision of free mobile medical services, especially at the landfill sites, would also improve curative treatment among scavengers.
A Systematic Review and Meta-Analysis on the Association and Differences between Aerobic Threshold and Point of Optimal Fat Oxidation
Authors: Peric Ratko , Nikolovski Zoran , Meucci Marco , Tadger Philippe , Ferri Marini Carlo , Amaro-Gahete Francisco José
Published: May 2022

Over the past two decades, scientists have attempted to evaluate whether the point of maximal fat oxidation (FATmax) and the aerobic threshold (AerT) are connected. The existence of such a relationship would allow a more tailored training approach for athletes while improving the efficacy of individualized exercise prescriptions when treating numerous health-related issues. However, studies have reported conflicting results, and this issue remains unresolved. This systematic review and meta-analysis aimed: (i) to examine the strength of the association between FATmax and AerT by using the effect size (ES) of correlation coefficient (r) and standardized mean difference (SMD); (ii) to identify potential moderators and their influence on ES variability. This study was registered with PROSPERO (CRD42021239351) and ClinicalTrials (NCT03789045). PubMed and Google Scholar were searched and fourteen articles, consisting of overall 35 ES for r and 26 ES for SMD were included. Obtained ESs were analyzed using a multilevel random-effects meta-analysis. Our results support the presence of a significant association between FATmax and AerT exercise intensities. In conclusion, due to the large ES variance caused by clinical and methodological differences among the studies, we recommend that future studies follow strict standardization of data collection and analysis of FATmax and AerT-related outcomes.
Circadian Variation of Blood Pressure in Patients with Chronic Musculoskeletal Pain: A Cross-Sectional Study
Authors: Navarro-Ledesma Santiago , Gonzalez-Muñoz Ana , García Ríos Maria , de la Serna Daniel , Pruimboom Leo
Published: May 2022

The aim of this study was to analyze the impact of circadian variation of blood pressure (BP) in patients with chronic musculoskeletal pain (CPM). A further purpose was to study differences in circadian variation of BP between genders and the correlation between BP circadian variation and pain. We performed a cross-sectional, observational study in which seventy-five participants with CMP participated. Circadian variation in BP was calculated using the diurnal/nocturnal BP ratio, and all participants used validated self-measurement BP devices. The Numeric Pain Rating Scale was used to assess pain perception. All circadian BP values from participants who suffered from CPM followed pathologic cardiovascular parameters (BP ratio < 10%). When comparing BP ratios between genders, statistically significant differences were found (p = 0.011). BP itself did not correlate with pain in any subgroup. Circadian variations of BP in those suffering from CMP are shown and new possibilities of research and treatment are proposed.
The Improved Proximal Phalanx Osteotomy for Reducing Sesamoid in Hallux Valgus Surgery—A Cadaver Study
Authors: Lee Dong-Oh , Hong Eunah , Kwak Dai-Soon
Published: May 2022

A metatarsal osteotomy is known to have the effect of reduction of the sesamoid. However, the reduction of the sesamoid is not always completed by a metatarsal osteotomy alone. The purpose of this cadaver study was to show that the improved technique of a modified Akin proximal phalanx osteotomy (MPO) could be helpful for the reduction of the sesamoids in hallux valgus surgery. Ten feet of cadavers were used; the cadavers had hallux valgus on both feet. The first trial of two feet underwent only the MPO. The other eight feet underwent a proximal metatarsal chevron osteotomy and MPO simultaneously. The hallux valgus angle, intermetatarsal angle, Hardy’s grade, and Smith’s grade were measured. To predict possible complications, cadavers were dissected after surgery. In the feet that underwent an MPO only, the hallux valgus angle and sesamoid position were improved. In the feet that underwent an MPO and metatarsal osteotomy, the hallux valgus deformity was completely corrected, and the sesamoid position was improved. Overall, the hallux valgus angle and intermetatarsal angle improved from 30.6 to 8.4 degrees and from 11.2 to 4.1 degrees, respectively. The sesamoid position was reduced from 5.3 to 2.5 (Hardy) and from 1.7 to 0.7 (Smith). The MPO combined with the metatarsal osteotomy were helpful for reducing the sesamoids compared to the metatarsal osteotomy only.
Digital Economy, Agricultural Technological Progress, and Agricultural Carbon Intensity: Evidence from China
Authors: Zhong Ruoxi , He Qiang , Qi Yanbin
Published: May 2022

China is the largest carbon emitter in the world, with agricultural carbon emissions accounting for 17% of China’s total carbon emissions. Agricultural carbon emission reduction has become the key to achieving the “Double Carbon” goal. At the same time, the role of the digital economy in achieving the “dual carbon” goal cannot be ignored as an important engine to boost the high-quality development of China’s economy. Therefore, this paper uses the panel data of 30 provinces in mainland China from 2011 to 2019 to construct a spatial Durbin model and a mediation effect model to explore the impact of the digital economy on agricultural carbon intensity and the mediating role of agricultural technological progress. The research results show that: (1) China’s agricultural carbon intensity fluctuated and declined during the study period, but the current agricultural carbon intensity is still at a high level; (2) The inhibitory effect of the digital economy on agricultural carbon intensity is achieved by promoting agricultural technological progress, and the intermediary role of agricultural technological progress has been verified; (3) The digital economy can significantly reduce the carbon intensity of agriculture, and this inhibition has a positive spatial spillover effect. According to the research conclusions, the government should speed up the development of internet technology and digital inclusive finance, support agricultural technology research and improve farmers’ human capital, and strengthen regional cooperation to release the contribution of digital economy space.
Pollution Characteristics and Spatial Distribution of Heavy Metals in Coal-Bearing Sandstone Soil: A Case Study of Coal Mine Area in Southwest China
Authors: Deng Dongping , Wu Yong , Sun Yi , Ren Bangzheng , Song Lei
Published: May 2022

Soil pollution in coal mining areas is a serious environmental problem in China and elsewhere. In this study, surface and vertical profile soil samples were collected from a coal mine area in Dazhu, Southwestern China. Microscopic observation, concentrations, chemical speciation, statistical analysis, spatial distribution, and risk assessment were used to assess heavy metal pollution. The results show that the weathering of coal-bearing sandstone and mining activities substantially contributed to soil pollution. The concentrations of Fe, Ni, Cu, Zn, Mn, Cd, Hg, and Pb exceeded their background values. Cd caused the most intense pollution and was associated with heavily–extremely contaminated soils. The residual fraction was dominant for most metals, except Cd and Mn, for which the reducible fraction was dominant (Cd: 55.17%; Mn: 81.16%). Zn, Ni, Cd, and Cu presented similar distribution patterns, and Hg and As also shared similar distribution characteristics. Factor 1 represented anthropogenic and lithologic sources, which were affected by mining activities; Factor 2 represented anthropogenic sources, e.g., fertilizers and traffic pollution; and Factor 3 represented the contribution of metals from soil-forming parent material. More than half of the study area had high pollution risk and was not suitable for vegetable cultivation.
Intercomparison of Local Warming Trends of Shanghai and Hong Kong Based on 120-Year Temperature Observational Data
Authors: Yang Yawei , Li Lei , Chan Pak-Wai , Zhou Qianjin , Sheng Bosi
Published: May 2022

Using surface air temperature observations from 1901 to 2020, this study compared the warming trends of Shanghai and Hong Kong over a period of 120 years. The statistical results reveal the following: (1) The average temperatures of the two cities underwent fluctuating increases during the past 120 years, with linear warming rates of 0.23 °C/decade in Shanghai and 0.13 °C/decade in Hong Kong. (2) The fluctuation ranges of maximum temperature in the two cities were considerably higher than those of mean temperature. Moreover, in both cities, the annual mean maximum temperature decreased during a phase of more than a decade. The fluctuation ranges of minimum temperature were smaller, whereas the linear increases were higher than those for the mean temperature. (3) The diurnal temperature ranges (DTRs) of the two cities decreased; a certain phase of the decreases in DTR in the two cities was caused by decreases in the maximum temperature. (4) At a certain stage of urban development, owing to the shading effect of new high-rise buildings, the solar shortwave radiation reaching the Earth’s surface decreased, and anthropogenic heat generated by the energy consumption of buildings and urban human activities at that time was not sufficient to make up for the reduced shortwave radiation. This result may have led to the declines in the maximum temperature experienced by both cities. (5) Currently, the number of hot days and extremely hot days in the two cities has increased significantly compared with that a century ago, indicating that climate warming has an adverse impact on human settlements.
Proteoglycans: Systems-Level Insight into Their Expression in Healthy and Diseased Placentas
Authors: Oravecz Orsolya , Balogh Andrea , Romero Roberto , Xu Yi , Juhasz Kata , Gelencser Zsolt , Xu Zhonghui , Bhatti Gaurav , Pique-Regi Roger , Peterfia Balint , Hupuczi Petronella , Kovalszky Ilona , Murthi Padma , Tarca Adi L. , Papp Zoltan , Matko Janos , Than Nandor Gabor
Published: May 2022

Proteoglycan macromolecules play key roles in several physiological processes (e.g., adhesion, proliferation, migration, invasion, angiogenesis, and apoptosis), all of which are important for placentation and healthy pregnancy. However, their precise roles in human reproduction have not been clarified. To fill this gap, herein, we provide an overview of the proteoglycans’ expression and role in the placenta, in trophoblast development, and in pregnancy complications (pre-eclampsia, fetal growth restriction), highlighting one of the most important members of this family, syndecan-1 (SDC1). Microarray data analysis showed that of 34 placentally expressed proteoglycans, SDC1 production is markedly the highest in the placenta and that SDC1 is the most upregulated gene during trophoblast differentiation into the syncytiotrophoblast. Furthermore, placental transcriptomic data identified dysregulated proteoglycan genes in pre-eclampsia and in fetal growth restriction, including SDC1, which is supported by the lower concentration of syndecan-1 in maternal blood in these syndromes. Overall, our clinical and in vitro studies, data analyses, and literature search pointed out that proteoglycans, as important components of the placenta, may regulate various stages of placental development and participate in the maintenance of a healthy pregnancy. Moreover, syndecan-1 may serve as a useful marker of syncytialization and a prognostic marker of adverse pregnancy outcomes. Further studies are warranted to explore the role of proteoglycans in healthy and complicated pregnancies, which may help in diagnostic or therapeutic developments.
Histone Modifications and Non-Coding RNAs: Mutual Epigenetic Regulation and Role in Pathogenesis
Authors: Bure Irina V. , Nemtsova Marina V. , Kuznetsova Ekaterina B.
Published: May 2022

In the last few years, more and more scientists have suggested and confirmed that epigenetic regulators are tightly connected and form a comprehensive network of regulatory pathways and feedback loops. This is particularly interesting for a better understanding of processes that occur in the development and progression of various diseases. Appearing on the preclinical stages of diseases, epigenetic aberrations may be prominent biomarkers. Being dynamic and reversible, epigenetic modifications could become targets for a novel option for therapy. Therefore, in this review, we are focusing on histone modifications and ncRNAs, their mutual regulation, role in cellular processes and potential clinical application.
Bone Involvement in Systemic Lupus Erythematosus
Authors: Rella Valeria , Rotondo Cinzia , Altomare Alberto , Cantatore Francesco Paolo , Corrado Addolorata
Published: May 2022

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by a wide variability of clinical manifestations due to the potential involvement of several tissues and internal organs, with a relapsing and remitting course. Dysregulation of innate and adaptive immune systems, due to genetic, hormonal and environmental factors, may be responsible for a broad spectrum of clinical manifestations, affecting quality of life, morbidity and mortality. Bone involvement represents one of the most common cause of morbidity and disability in SLE. Particularly, an increased incidence of osteoporosis, avascular necrosis of bone and osteomyelitis has been observed in SLE patients compared to the general population. Moreover, due to the improvement in diagnosis and therapy, the survival of SLE patient has improved, increasing long-term morbidities, including osteoporosis and related fractures. This review aims to highlight bone manifestations in SLE patients, deepening underlying etiopathogenetic mechanisms, diagnostic tools and available treatment.
In Vitro Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study
Authors: Hihara Fukiko , Matsumoto Hiroki , Yoshimoto Mitsuyoshi , Masuko Takashi , Endo Yuichi , Igarashi Chika , Tachibana Tomoko , Shinada Mitsuhiro , Zhang Ming-Rong , Kurosawa Gene , Sugyo Aya , Tsuji Atsushi B. , Higashi Tatsuya , Kurihara Hiroaki , Ueno Makoto , Yoshii Yukie
Published: May 2022

Peritoneal dissemination of pancreatic cancer has a poor prognosis. We have reported that intraperitoneal radioimmunotherapy using a 64Cu-labeled antibody (64Cu-ipRIT) is a promising adjuvant therapy option to prevent this complication. To achieve personalized 64Cu-ipRIT, we developed a new in vitro tumor cell-binding assay (64Cu-TuBA) system with a panel containing nine candidate 64Cu-labeled antibodies targeting seven antigens (EGFR, HER2, HER3, TfR, EpCAM, LAT1, and CD98), which are reportedly overexpressed in patients with pancreatic cancer. We investigated the feasibility of 64Cu-TuBA to select the highest-binding antibody for individual cancer cell lines and predict the treatment response in vivo for 64Cu-ipRIT. 64Cu-TuBA was performed using six human pancreatic cancer cell lines. For three cell lines, an in vivo treatment study was performed with 64Cu-ipRIT using high-, middle-, or low-binding antibodies in each peritoneal dissemination mouse model. The high-binding antibodies significantly prolonged survival in each mouse model, while low-and middle-binding antibodies were ineffective. There was a correlation between in vitro cell binding and in vivo therapeutic efficacy. Our findings suggest that 64Cu-TuBA can be used for patient selection to enable personalized 64Cu-ipRIT. Tumor cells isolated from surgically resected tumor tissues would be suitable for analysis with the 64Cu-TuBA system in future clinical studies.
Dynamic Changes in Ascorbic Acid Content during Fruit Development and Ripening of Actinidia latifolia (an Ascorbate-Rich Fruit Crop) and the Associated Molecular Mechanisms
Authors: Deng Honghong , Xia Hui , Guo Yuqi , Liu Xinling , Lin Lijin , Wang Jin , Xu Kunfu , Lv Xiulan , Hu Rongping , Liang Dong
Published: May 2022

Actinidia latifolia is one of the very few kiwifruit genotypes with extremely high ascorbic acid (AsA) content. However, a transcriptome atlas of this species is lacking. The accumulation of AsA during fruit development and ripening and the associated molecular mechanisms are still poorly understood. Herein, dynamic changes in AsA content at six different stages of A. latifolia fruit development and ripening were determined. AsA content of A. latifolia fruit reached 1108.76 ± 35.26 mg 100 g−1 FW at full maturity. A high-quality, full-length (FL) transcriptome of A. latifolia was successfully constructed for the first time using third-generation sequencing technology. The transcriptome comprises 326,926 FL non-chimeric reads, 15,505 coding sequences, 2882 transcription factors, 18,797 simple sequence repeats, 3328 long noncoding RNAs, and 231 alternative splicing events. The genes involved in AsA biosynthesis and recycling pathways were identified and compared with those in different kiwifruit genotypes. The correlation between the AsA content and expression levels of key genes in AsA biosynthesis and recycling pathways was revealed. LncRNAs that participate in AsA-related gene expression regulation were also identified. Gene expression patterns in AsA biosynthesis and metabolism exhibited a trend similar to that of AsA accumulation. Overall, this study paves the way for genetic engineering to develop kiwifruits with super-high AsA content.
The Role of SBI2/ALG12/EBS4 in the Regulation of Endoplasmic Reticulum-Associated Degradation (ERAD) Studied by a Null Allele
Authors: Sun Xiaoxia , Zhao Di , Ali Khawar , Zhu Yumeng , Wu Guang , Li Guishuang
Published: May 2022

Redundancy and lethality is a long-standing problem in genetics but generating minimal and lethal phenotypes in the knockouts of the same gene by different approaches drives this problem to a new high. In Asn (N)-linked glycosylation, a complex and ubiquitous cotranslational and post-translational protein modification required for the transfer of correctly folded proteins and endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins, ALG12 (EBS4) is an α 1, 6-mannosyltransferase catalyzing a mannose into Glc3Man9GlcNAc2. In Arabidopsis, T-DNA knockout alg12-T is lethal while likely ebs4 null mutants isolated by forward genetics are most healthy as weak alleles, perplexing researchers and demanding further investigations. Here, we isolated a true null allele, sbi2, with the W258Stop mutation in ALG12/EBS4. sbi2 restored the sensitivity of brassinosteroid receptor mutants bri1-5, bri1-9, and bri1-235 with ER-trapped BRI1 to brassinosteroids. Furthermore, sbi2 maturated earlier than the wild-type. Moreover, concomitant with impaired and misfolded proteins accumulated in the ER, sbi2 had higher sensitivity to tunicamycin and salt than the wild-type. Our findings thus clarify the role of SBI2/ALG12/EBS4 in the regulation of the ERAD of misfolded glycoproteins, and plant growth and stress response. Further, our study advocates the necessity and importance of using multiple approaches to validate genetics study.
Hypertension in Patients with Insulin Resistance: Etiopathogenesis and Management in Children
Authors: Tagi Veronica Maria , Mainieri Francesca , Chiarelli Francesco
Published: May 2022

Insulin resistance (IR) is a key component in the etiopathogenesis of hypertension (HS) in patients with diabetes mellitus (DM). Several pathways have been found to be involved in this mechanism in recent literature. For the above-mentioned reasons, treatment of HS should be specifically addressed in patients affected by DM. Two relevant recently published guidelines have stressed this concept, giving specific advice in the treatment of HS in children belonging to this group: the European Society of HS guidelines for the management of high blood pressure in children and adolescents and the American Academy of Pediatrics Clinical Practice Guideline for Screening and Management of High Blood Pressure in Children and Adolescents. Our aim is to summarize the main pathophysiological mechanisms through which IR causes HS and to highlight the specific principles of treatment of HS for children with DM.
Recent Advances of m6A Demethylases Inhibitors and Their Biological Functions in Human Diseases
Authors: You Yazhen , Fu Yundong , Huang Mingjie , Shen Dandan , Zhao Bing , Liu Hongmin , Zheng Yichao , Huang Lihua
Published: May 2022

N6-methyladenosine (m6A) is a post-transcriptional RNA modification and one of the most abundant types of RNA chemical modifications. m6A functions as a molecular switch and is involved in a range of biomedical aspects, including cardiovascular diseases, the central nervous system, and cancers. Conceptually, m6A methylation can be dynamically and reversibly modulated by RNA methylation regulatory proteins, resulting in diverse fates of mRNAs. This review focuses on m6A demethylases fat-mass- and obesity-associated protein (FTO) and alkB homolog 5 (ALKBH5), which especially erase m6A modification from target mRNAs. Recent advances have highlighted that FTO and ALKBH5 play an oncogenic role in various cancers, such as acute myeloid leukemias (AML), glioblastoma, and breast cancer. Moreover, studies in vitro and in mouse models confirmed that FTO-specific inhibitors exhibited anti-tumor effects in several cancers. Accumulating evidence has suggested the possibility of FTO and ALKBH5 as therapeutic targets for specific diseases. In this review, we aim to illustrate the structural properties of these two m6A demethylases and the development of their specific inhibitors. Additionally, this review will summarize the biological functions of these two m6A demethylases in various types of cancers and other human diseases.