bluuebunny/crossref_metadata_2025 huggingface dataset:
SERINC5 counters retroviruses and non-retroviruses
Authors: Yu Jinghua , Liu Chunyu , Qu Xinglong , Gao Xinglin , Liu Yue
Published: January 2025

SERINC5 (serine incorporator 5), a member of the serine incorporator family, has been identified as a retrovirus restriction factor that inhibits the fusion of virions with the plasma membrane, thus blocking the release of the viral core into target cells and subsequently attenuating viral infectivity. Several viruses, such as human immunodeficiency virus (HIV), murine leukemia virus (MLV), and equine infectious anemia virus (EIAV), have evolved mechanisms to antagonize the host protein SERINC5 through HIV Nef, MLV glycosylated Gag, and the EIAV S2 protein. These viral proteins degrade SERINC5 on the cell surface through the endolysosomal system. In addition to its direct antiviral ability, SERINC5 also modulates immunity to inhibit the replication of retroviruses and nonretroviruses. This review summarizes the interaction between SERINC5 and viral replication, providing a promising avenue for fighting viral diseases.
Connecting climate science and society: reflections from early and mid-career researchers at the World Climate Research Programme Open Science Conference 2023
Authors: Díaz Leandro B. , Gulizia Carla , Jain Shipra , Langendijk Gaby S. , Palanisamy Hindumathi , Rabanal Valentina , Charlton Candice S. , Dajuma Alima , Donkor Felix K. , Egbebiyi Temitope S. , Kondi Akara Ghafi , Jrrar Amna , Siddiqui Md Rezwan , Brahim Yassine Ait , Angnuureng Bapentire D. , Anschütz Anna-Adriana , Awo Founi M. , Builes-Jaramillo Alejandro , Cobb Alison , Diallo Mohamadou A. , Er-Retby Houda , Fotso-Nguemo Thierry C. , Gwinneth Benjamin , Gudoshava Masilin , Kemgang Ghomsi Franck E. , Leyba Inés M. , Lguensat Redouane , Mahon Roché , Mandal Shailendra K. , Mumo Lucia , Muzuva Meshel , Nonki Rodric M. , Rivera Juan A. , Segnon Alcade C. , Tanimoune Laouali I. , Zauisomue Erlich-Honest E. I. , Siame Gilbert
Published: January 2025

This paper reflects the discussions of early and mid-career researchers (EMCRs) during the World Climate Research Programme Open Science Conference 2023 EMCRs Symposium, to advance climate knowledge for greater transformative power in society and impact on policy-making. These discussions focused on three key priority challenges: how to produce robust, usable, and used climate information at the local scale; how to address key climate research and knowledge gaps in the Global South; and how EMCRs could support policy-making with climate information. We present here our perspective on these major challenges, possible ways to address them, and what could be the contribution of EMCRs. In addition, we provide recommendations for actions that could be taken at the international and national levels to increase the voice and leadership of Global South researchers and EMCRs in international scientific endeavors. These recommendations might facilitate the integration of new technological tools or innovative approaches, promote interdisciplinary collaboration, and foster connections with local communities. This coordinated approach to international, regional and local initiatives will catalyze the process for urgent action on the environmental crisis before us.
Case Report: A case of Tatton-Brown–Rahman syndrome featuring mitral annular disjunction and mitral valve prolapse due to a novel mutation site in the DNMT3A gene
Authors: Xu Zhong-jiao , Shen Ru-ming , Hu Wu-ming , Lv Lin-chun , Shi Zhen-hua , Lin Li
Published: January 2025

A 13-year-old child presented with specific facial features, overgrowth, and intellectual disability. Echocardiography revealed the presence of a large pericardial effusion, left ventricular enlargement, mitral annular separation, and mitral valve prolapse with moderate regurgitation. These symptoms suggested a possible genetic disorder. High-throughput sequencing revealed a specific mutation in the DNMT3A gene (NM_175629.2:c.2408 + 1G > A) associated with Tatton-Brown–Rahman syndrome. The patient's condition was alleviated through accurate diagnosis and comprehensive treatment measures, including psychological and social support. Regular follow-ups to monitor the disease's progress and the effectiveness of treatment, along with timely adjustments to the treatment plan, can not only effectively reduce the child's symptoms and improve their quality of life but may also help prevent the potential risk of sudden death.
Associations between accurate measures of adiposity and fitness, blood proteins, and insulin sensitivity among South Asians and Europeans
Authors: Kho Pik Fang , Stell Laurel , Jimenez Shirin , Zanetti Daniela , Panyard Daniel J. , Watson Kathleen L. , Sarraju Ashish , Chen Ming-Li , Lind Lars , Petrie John R. , Chan Khin N. , Fonda Holly , Kent Kyla , Myers Jonathan N. , Palaniappan Latha , Abbasi Fahim , Assimes Themistocles L.
Published: January 2025

Objective

South Asians (SAs) may possess a unique predisposition to insulin resistance (IR). We explored this possibility by investigating the relationship between ‘gold standard’ measures of adiposity, fitness, selected proteomic biomarkers, and insulin sensitivity among a cohort of SAs and Europeans (EURs).

Methods

A total of 46 SAs and 41 EURs completed ‘conventional’ (lifestyle questionnaires, standard physical exam) as well as ‘gold standard’ (dual energy X-ray absorptiometry scan, cardiopulmonary exercise test, and insulin suppression test) assessments of adiposity, fitness, and insulin sensitivity. In a subset of 28 SAs and 36 EURs, we also measured the blood-levels of eleven IR-related proteins. We conducted Spearman correlation to identify correlates of steady-state plasma glucose (SSPG) derived from the insulin suppression test, followed by multivariable linear regression analyses of SSPG, adjusting for age, sex and ancestral group.

Results

Sixteen of 30 measures significantly associated with SSPG, including one conventional and eight gold standard measures of adiposity, one conventional and one gold standard measure of fitness, and five proteins. Multivariable regressions revealed that gold standard measures and plasma proteins attenuated ancestral group differences in IR, suggesting their potential utility in assessing IR, especially among SAs.

Conclusion

Ancestral group differences in IR may be explained by accurate measures of adiposity and fitness, with specific proteins possibly serving as useful surrogates for these measures, particularly for SAs.
Longitudinal analysis of soil erosion dynamics using the RUSLE model in Ethiopia’s Lake Ziway watershed: implications for agricultural sustainability and food security
Authors: Teku Degfie , Workie Melak Desta
Published: January 2025

Introduction

Soil erosion is a persistent environmental challenge in Ethiopia’s Lake Ziway Watershed, posing severe risks to agricultural sustainability, food security, and ecosystem stability. Despite its significance, limited longitudinal studies have systematically analyzed soil erosion dynamics in this region or identified critical intervention areas.

Methods

This study employs the Revised Universal Soil Loss Equation (RUSLE) model integrated with remote sensing and GIS techniques to perform a 30-year longitudinal analysis (1993–2023).

Results

The analysis highlights spatial and temporal trends in both potential and modeled actual soil erosion rates, revealing substantial but fluctuating losses over the study period. In 1993, potential soil erosion ranged from 0 to 2,082 t/ha/yr, totaling approximately 3.76 million tonnes annually. By 2023, this had shifted to a range of 0–2,069 t/ha/yr, with an annual loss of 3.48 million tonnes. Modeled actual soil erosion followed a similar trajectory, decreasing from 2.58 million tonnes in 1993 to 2.30 million tonnes in 2023. Mean soil loss rates for potential erosion increased from 42.91 t/ha/yr in 1993 to 43.73 t/ha/yr in 2023, while modeled actual erosion rates rose from 38.79 t/ha/yr to 41.01 t/ha/yr.

Discussion

These figures consistently exceed Ethiopia’s acceptable soil loss threshold of 10–15 t/ha/y, highlighting the need for targeted interventions, particularly in erosion-prone areas. This study bridges critical knowledge gaps, providing actionable insights for policy development aimed at sustainable land management and agricultural resilience.
Converting infiltration swales to sustainable urban drainage systems can improve water management and biodiversity
Authors: Helmreich Brigitte , Deeb Maha , Eben Patrizia , Egerer Monika , Kollmann Johannes , Schulz Stefanie , Pauleit Stephan , Weisser Wolfgang W. , Schloter Michael
Published: January 2025

Sustainable Urban Drainage Systems (SUDS) are ecosystems that are based on engineered soil and designed plant communities to manage stormwater on-site and to enhance infiltration, evapotranspiration, and cooling, thus reducing flooding and urban heat islands. In addition, SUDS may act as hotspots for biodiversity and could be more socially accepted if they work well and are multifunctional. However, we still lack a critical understanding of the techno-ecological basis to construct SUDS sustainably. Due to climate change and pollutants such as de-icing salts, SUDS are confronted with harmful environmental triggers that interfere with their sustainable development. Thus, the challenge is to combine stormwater treatment and urban drainage with principles of restoration ecology, while implementing expertise from soil science, microbiome research, and plant ecology. In this perspective paper, we will discuss the SUDS development and maintenance principle and the role of interdisciplinary research in reaching these goals.
Treatment of hypotrichosis simplex of the scalp with the combination of botanic extracts and minoxidil: a case report
Authors: Zhuang Mingyue , Xu Yonglong , Zhong Shiyi , Tian Ziyuan , Liu Qingwu , Yang Dingquan
Published: January 2025

Hypotrichosis simplex of the scalp (HSS) is a clinically rare monogenic autosomal dominant disorder associated with variants in the gene CDSN, which encodes the desmosome protein corneodesmosin. Although studies have reported that some medications can improve the symptoms of hair loss in HSS, there is still a lack of definitive and effective treatments for this disease. We report a familial case of HSS in an 8-year-old male child diagnosed with HSS caused by a mutation in CDSN, who was treated with botanical extracts in combination with minoxidil, which resulted in significant hair growth after two treatments. This is the first study describing the improvement of clinical symptoms of HSS with oral botanical extracts. This suggests that botanical extracts in combination with minoxidil may be a therapeutic approach for HSS in the clinic.
Mutation in the COL2A1 gene is associated with acetabular dysplasia
Authors: Xin Miaomiao , Guan Xin , Yang Jiangfei , Li Yi , Man Zhentao , Sun Hongsheng , Fu Min
Published: January 2025

Background

Developmental dysplasia of the hip (DDH) is one of the most common developmental disorders worldwide, caused by a combination of genetic and environmental factors.

Methods

To investigate the genetic etiology of DDH in a proband (a 27-year-old male), we reviewed the patient’s clinical data and collected peripheral blood samples from the proband and his parents. Genomic DNA was extracted, and polymerase chain reaction (PCR) amplification was performed. Clinical whole-exome sequencing (WES) using next-generation sequencing (NGS) was conducted to identify potential mutation sites, which were then validated through Sanger sequencing. Bioinformatics analysis was performed to assess the pathogenicity of the identified variant, and 3D protein modeling was conducted to predict its impact on protein structure.

Results

The proband presented with pain in bilateral hips, and based on clinical symptoms, laboratory findings and imaging studies, the final diagnosis was considered to be acetabular dysplasia with overlapping secondary synovial chondromatosis. Family history revealed similar symptoms in the proband’s father, while the grandparents and other family members were unaffected. The patient underwent bilateral total hip arthroplasty and synovectomy. NGS and Sanger sequencing identified a heterozygous missense mutation in the COL2A1 gene (ex13, c.823C > T; p.Arg275Cys) in both the proband and his father, while this mutation was absent in the mother. Bioinformatic analysis indicated that the c.823C > T (p.Arg275Cys) variant is pathogenic, and structural modeling demonstrated that the substitution of arginine with cysteine at residue 275 altered the protein structure.

Conclusion

Our findings highlight the diagnostic utility of NGS in identifying precise genetic causes of DDH. The identification of the COL2A1 gene mutation in this present case represents a novel clinical phenotype, expanding the spectrum of disorders associated with COL2A1 mutations.
A practical guide to FAIR data management in the age of multi-OMICS and AI
Authors: Mugahid Douaa , Lyon Jared , Demurjian Charlie , Eolin Nathan , Whittaker Charlie , Godek Mark , Lauffenburger Douglas , Fortune Sarah , Levine Stuart
Published: January 2025

Multi-cellular biological systems, including the immune system, are highly complex, dynamic, and adaptable. Systems biologists aim to understand such complexity at a quantitative level. However, these ambitious efforts are often limited by access to a variety of high-density intra-, extra- and multi-cellular measurements resolved in time and space and across a variety of perturbations. The advent of automation, OMICs and single-cell technologies now allows high dimensional multi-modal data acquisition from the same biological samples multiplexed at scale (multi-OMICs). As a result, systems biologists -theoretically- have access to more data than ever. However, the mathematical frameworks and computational tools needed to analyze and interpret such data are often still nascent, limiting the biological insights that can be obtained without years of computational method development and validation. More pressingly, much of the data sits in silos in formats that are incomprehensible to other scientists or machines limiting its value to the vaster scientific community, especially the computational biologists tasked with analyzing these vast amounts of data in more nuanced ways. With the rapid development and increasing interest in using artificial intelligence (AI) for the life sciences, improving how biologic data is organized and shared is more pressing than ever for scientific progress. Here, we outline a practical approach to multi-modal data management and FAIR sharing, which are in line with the latest US and EU funders’ data sharing policies. This framework can help extend the longevity and utility of data by allowing facile use and reuse, accelerating scientific discovery in the biomedical sciences.
Effect of trace elements and nutrients on 21 autoimmune diseases: a Mendelian randomization study
Authors: Jia Ming-Jie , Yin Hua-Fang , Liang Ying-Chao , Jiang Feng , Li Hui-Lin
Published: January 2025

Background

Numerous clinical studies have observed a close relationship between serum trace elements, nutrients, and autoimmune diseases. However, whether there is a genetic causal effect between serum trace elements, nutrients, and autoimmune diseases remains unclear.

Objective

This study aims to investigate the causal effects of serum trace elements and nutrients on 21 autoimmune diseases using Mendelian randomization (MR).

Methods

Single nucleotide polymorphisms for the exposure factors (serum trace elements and vitamins) were obtained from the published UK Biobank database and genome-wide association study (GWAS) public databases. Outcome GWAS data were derived from the FinnGen database. MR was employed to explore the causal relationships between 9 trace elements and 6 vitamins and autoimmune diseases. Causal inference was performed using inverse variance weighted methods, MR Egger, and weighted median methods. Subsequently, heterogeneity tests, horizontal pleiotropy tests, MR-PRESSO tests, and leave-one-out analyses were conducted for sensitivity analysis to evaluate the robustness of the study results. Finally, trace elements and vitamins that were statistically significant in the IVW method and had consistent effect sizes and odds ratios across five methods were selected as exposure factors with a causal relationship to diabetes and its complications. Additionally, multivariable Mendelian randomization was employed to assess the combined effects of multiple exposure factors on autoimmune diseases.

Results

MR analysis indicated that elevated levels of the trace element copper were associated with an increased risk of systemic lupus erythematosus (SLE) and a decreased risk of ulcerative colitis. Carotene was found to have a negative causal relationship with adult-onset Still’s disease (AOSD). Elevated levels of copper and selenium were associated with an increased risk of autoimmune hyperthyroidism. Calcium levels showed a negative causal relationship with the risk of polyarteritis nodosa. MVMR results demonstrated that selenium could independently affect the risk of autoimmune hyperthyroidism, separate from copper.

Conclusion

The findings from both univariable and multivariable Mendelian randomization studies support a causal relationship between trace elements, nutrients, and autoimmune diseases. These results have significant clinical implications for developing targeted prevention and treatment strategies for autoimmune diseases.
The generation and evaluation of TKO/hCD55/hTM/hEPCR gene-modified pigs for clinical organ xenotransplantation
Authors: Huai Guoli , Wang Yong , Du Jiaxiang , Cheng Zhenhui , Xie Yuxuan , Zhou Jia , Tang Hongmei , Jiang Yanyan , Xing Xiangyang , Deng Shaoping , Pan Dengke
Published: January 2025

Introduction

Genetically edited pigs, modified using CRISPR-Cas9 technology, hold promise as potential sources for xenotransplantation. However, the optimal combination of genetic modifications and their expression levels for initial clinical trials remains unclear. This study investigates the generation of TKO/hCD55/hTM/hEPCR (6GE) pigs and evaluates their compatibility with human immune and coagulation systems.

Methods

The 6GE pigs were generated through iterative genome editing and F1 generation breeding. Genotyping, flow cytometry, and immunohistochemistry confirmed the knockout of GGTA1, CMAH, and B4GALNT2. Expression levels of human genes (hCD55, hTM, hEPCR) were quantified. In vitro assays using aortic endothelial cells (pAECs) from 6GE pigs assessed human serum IgM and IgG binding, complement cytotoxicity, and thrombin-antithrombin (TAT) complex levels. Blood from gene-edited pigs was used for pathophysiological analysis.

Results

Complete knockout of GGTA1, CMAH, and B4GALNT2 was confirmed in 6GE pigs. The expression of hCD55 and hTM was approximately seven and thirteen times higher than in humans, respectively, while hEPCR levels were comparable to those in humans. In vitro, 6GE pAECs showed significantly reduced binding of human IgM and IgG compared to wild-type pAECs (IgG p<0.01, IgM p<0.0001). Similar to TKO/hCD55 pAECs, 6GE pAECs exhibited a substantial reduction in complement-mediated cytotoxicity (p<0.001) compared to TKO pAECs. Co-expression of hTM and hEPCR in 6GE pigs led to a significant decrease in thrombin-antithrombin (TAT) complex levels in co-culture with human whole blood, compared to WT (p<0.0001), TKO (p<0.01), and TKO/hCD55/hTM pigs (p<0.05). Pathophysiological analysis demonstrated excellent compatibility of 6GE pig kidneys and livers with human immune and coagulation systems. However, 6GE pigs showed increased susceptibility to infection compared to other gene-edited pigs, while TKO/hCD55 pigs were considered safe when they were all bred in a general environment.

Discussion

Highly expressing hCD55, along with the co-expression of hEPCR and hTM genes, is expected to effectively reduce human complement cytotoxicity and enhance anticoagulant efficacy in genetically modified pigs. The 6GE pigs exhibited robust compatibility with human physiological and immune systems, fulfilling the criteria for clinical trials. Furthermore, it is imperative to rear donor pigs in pathogen-free (DPF) facilities to mitigate infection risks and prevent the transmission of porcine pathogens to humans.
Heightened expression of type I interferon signaling genes in CD4+ T cells from acutely HIV-1–infected women is associated with lower viral loads
Authors: El-Badry Elina , Chen Luxiao , Ghneim Khader , Li Ziyi , Brooks Kelsie , Rhodes Jake , Sekaly Rafick , Kilembe William , Allen Susan , Wu Hao , Hunter Eric
Published: January 2025

Sex differences play a role in the pathogenesis of a number of viral diseases. In HIV-1, several studies have reported that chronically infected women have significantly lower plasma viremia than men, although the exact mechanism by which this occurs has yet to be identified. We have performed bulk RNA-seq experiments comparing gene expression between CD4+ T cells from acutely HIV-1–infected men and women in Zambia, because we observe lower viral load (VL) despite higher CD4+ T-cell activation in these women during acute/early infection. In a univariate analysis, we have identified a number of differentially expressed genes in naïve, central memory, and effector memory CD4 T cells of women with consistent elevated expression of genes linked to type 1 interferon (IFN) signaling. Moreover, after controlling for differences in VL and CD4+ T-cell count, genes within the type I IFN signaling pathway were further shown to be more highly expressed in women, whereas those genes more highly expressed in men showed no such enrichment. A subset of the genes highly expressed in women was further identified, including several involved in type I IFN signaling in response to viral infections (IRF7, DDX58, SAMHD1, OAS2, and TRIM14), that both are more highly expressed in CD4+ T cells from women and negatively correlated with VL, suggesting that they play a role in the comparative control of VL observed in women.
Infectious outcomes of a standardized subcutaneous immunoglobulin dose reduction strategy in primary immune deficiencies amid global shortage
Authors: Moral Moral Pedro , Cabanero-Navalon Marta Dafne , López-León Paula Teresa , Balastegui-Martín Héctor , Martínez Mercader Sandra , Mir Amparo , Garcia-Bustos Victor
Published: January 2025

Introduction

Immunoglobulin replacement therapy (IgRT), either intravenous (IVIg) or subcutaneous (SCIg), is crucial for managing primary immune deficiencies (PIDs) with hypogammaglobulinemia by reducing infection rates and mortality. During the COVID-19 pandemic, a global shortage of SCIg prompted our unit to reduce SCIg doses or maintain the same dose intravenously. This study evaluates the impact of a standardized SCIg dose reduction on infection rates and clinical outcomes in patients with humoral PID and with a low burden of infections.

Methods

Adult PID patients on SCIg for at least 6 months, with IgG trough levels ≥ 700 mg/dL (or ≥ 900 mg/dL under specific conditions), and no significant infections in the past 6 months were eligible. A dose reduction of 15 mg/kg/week (60 mg/kg/month) for every 150 mg/dL above 700 mg/dL (or 900 mg/dL) was proposed. Clinical and laboratory data, and infectious events at 6- and 12-month follow-ups, were analyzed.

Results

Thirty-one patients with PID were included: common variable immunodeficiency (54.83%), IgG subclass deficiency (9.67%), and other PIDs (35.48%). The average SCIg dose was initially reduced from 7.82 g/week to 5.72 g/week and adjusted to 6.94 g/week at 12 months. There was no significant change in severe or mild infections before and at 6- and 12-months post-dose adjustment. The dose reduction saved an average of 5,550 euros per patient annually, totaling 172,050 euros annually for our cohort.

Discussion

Optimizing SCIg doses in selected well-controlled humoral PIDs is feasible without increasing infection rates, conserving this plasma-derived product during shortages. Larger prospective studies are needed to confirm this strategy's utility and its application to other Ig formulations.
Cordyceps militaris solid medium extract alleviates lipopolysaccharide-induced acute lung injury via regulating gut microbiota and metabolism
Authors: Wang Xiaoya , Zhang Kang , Zhang Jingyan , Xu Guowei , Guo Zhiting , Lu Xiaorong , Liang Chunhua , Gu Xueyan , Huang Liping , Liu Shuqi , Wang Lei , Li Jianxi
Published: January 2025

Acute lung injury (ALI) is a common respiratory disease, Cordycepin has been reported to reduce ALI, which is an effective component in Cordyceps militaris solid medium extract (CMME). Therefore, we aimed to explore the alleviating effect and mechanism of CMME on ALI. This study evaluated the effect of CMME on lipopolysaccharide (LPS)-induced ALI mice by analyzing intestinal flora and metabolomics to explore its potential mechanism. We assessed pulmonary changes, inflammation, oxidative stress, and macrophage and neutrophil activation levels, then we analyzed the gut microbiota through 16S rRNA and analyzed metabolomics profile by UPLC-QTOF/MS. The results showed that CMME treatment improved pulmonary injury, reduced inflammatory factors and oxidative stress levels, and decreased macrophage activation and neutrophil recruitment. The 16S rRNA results revealed that CMME significantly increased gut microbiota richness and diversity and reduced the abundance of Bacteroides compared with Mod group significantly. Metabolic analysis indicated that CMME reversed the levels of differential metabolites and may ameliorate lung injury through purine metabolism, nucleotide metabolism, and bile acid (BA) metabolism, and CMME did reverse the changes of BA metabolites in ALI mice, and BA metabolites were associated with inflammatory factors and intestinal flora. Therefore, CMME may improve lung injury by regulating intestinal flora and correcting metabolic disorders, providing new insights into its mechanism of action.
Case report: Cutaneous metastasis of squamous cervical carcinoma: complete regression after molecular diagnosis
Authors: Guo Liwen , Liu Yanqiong , Zhang Shuhua , Liu Wei
Published: January 2025

Common metastasis sites for cervical cancer are the lungs, bones, liver, brain, ovaries, and lymph nodes, among other sites. Skin metastasis is very uncommon, and is only observed in approximately 1% of patients. The cancer spreads typically through lymphatic or blood vessels, but a definitive example of lymphatic spread has not been documented thoroughly in the existing literature. Cutaneous metastasis may be confused with cellulitis or a rash; hence, an immediate cutaneous biopsy of any suspicious lesions is recommended. There is no consensus regarding the treatment of this condition. Only one documented case has shown that a combination of paclitaxel, carboplatin, bevacizumab, and zoledronic acid can lead to a complete metabolic response. Our study, which used two cycles of albumin-bound paclitaxel, cisplatin, and bevacizumab, followed by four cycles of the same regimen plus terprelimab for metastases with CPS scores of Programmed death-ligand 1 (PD-L1) over 10, resulted in a stable complete response for over eight months. Our contribution may assist in formulating effective treatment guidelines for the cutaneous metastasis of squamous cervical carcinoma in the future.
The role of isoniazid dosage and NAT2 gene polymorphism in the treatment of tuberculous meningitis
Authors: Jian Yi , Bao Yuhang , Yang Fashuang , Zhu Mei
Published: January 2025

Background

Tuberculous meningitis (TBM) is a non-purulent inflammatory condition affecting the meninges and spinal membranes, caused by Mycobacterium tuberculosis (MTB) infection. This study seeks to explore the impact of varying INH dosages and NAT2 gene polymorphisms on TBM treatment, contributing new insights to improve clinical management and patient prognosis.

Methods

Patients with TBM hospitalized between July 2020 and December 2022 were categorized into two groups based on INH dosage: the standard-dose group (300 mg/day) and the high-dose group (600 mg/day). General and baseline data were collected, and NAT2 genotypes were identified using real-time fluorescent PCR with melting curve analysis. The clinical characteristics and outcomes of patients with TBM under varying INH dosages were analyzed.

Results

This study enrolled 119 patients with TBM, including 32 (26.9%) in the standard-dose group and 87 (73.1%) in the high-dose group. The NAT2 genotypes were distributed as follows: 34 (28.6%) fast acetylators (FA), 73 (61.3%) intermediate acetylators (IA), and 12 (10.1%) slow acetylators (SA). By month 12, 25 patients (21.0%) experienced disability or death, with 22 cases (18.5%) occurring by the end of the 3rd month. Disability and mortality rates differed significantly between the standard-dose and high-dose groups for IA-type TBM patients (P = 0.014). Univariate analysis showed significant differences between groups in baseline focal neurological impairment and disability or mortality by the 3rd and 12th months. Multivariate logistic regression identified INH dosage, cranial nerve palsy, age, and headache as key prognostic factors for TBM.

Conclusion

High-dose INH treatment was associated with a reduced incidence of disability or death compared to the standard-dose regimen, indicating better efficacy and prognosis. In patients with IA-type TBM, the high-dose group showed a significantly lower rate of disability or mortality, suggesting that higher INH dosages may reduce the risk of adverse outcomes.
Co-location of seaweed farming with offshore wind energy: a quick scoping review
Authors: Coffey Brianna , Borgerson Cortni , Lal Pankaj , Feehan Colette J.
Published: January 2025

Seaweed farming is increasingly recognized as a sustainable marine resource management opportunity, but it also poses socioeconomic and environmental risks that require careful evaluation. This quick scoping review (QSR) examines the current state of knowledge on upscaling seaweed farming through co-location with offshore wind energy production. A total of 240 published records from 2001 to 2022 were analyzed, including studies on general seaweed farming and its integration with offshore wind energy, both of which have shown a significant increase in annual publication rates over time. Geographically, the majority of studies on general seaweed farming were conducted in Asia, while most research on wind-focused integration was carried out in Europe. Differences in cultivated species were evident, with red seaweeds dominating the general literature and brown seaweeds dominating wind-focused studies. Ecosystem service analysis revealed that provisioning services were disproportionately emphasized, while cultural services were underrepresented in wind-focused studies as compared to the general literature. Environmental constraints were the most frequently cited challenges across both datasets, but their nature differed: general literature highlighted issues such as pests, diseases, and epiphytes that reduce farm yield, while wind-focused studies emphasized risks of farms to local species, habitats, and ecosystems. While environmental knowledge gaps were the most frequently cited overall, legal knowledge gaps were predominant in wind-focused studies. These findings underscore the need for more geographically and taxonomically diverse studies on seaweed-wind multi-use, along with further investigation into cultural services in offshore contexts, strategies for mitigating environmental risks, and the development of frameworks for shared governance to advance sustainable ocean development.
Are the economic valuations of marine and coastal ecosystem services supporting policymakers? A systematic review and remaining gaps and challenges
Authors: Pacifico Andrea Mattia , Mulazzani Luca , Malorgio Giulio
Published: January 2025

With the increasing adoption of the ecosystem approach as integral to sustainable development policies, the economic valuation of marine and coastal ecosystem services (ESs) has become relevant for informing decision-making processes. Through an integrated approach encompassing bibliometric, network, and content analyses, this review is aimed at analyzing the evolution trend, the main research clusters, and the research gaps of the scientific literature in the field of economic valuation of marine and coastal ESs. The bibliometric results showed that the research field is experiencing an evolving positive trend and represents a challenging research topic. From the network and overlay visualization of keyword co-occurrences, it emerged that the research clusters comprehensively address the key policy-relevant issues. In the content analysis, an examination of the estimated ESs and the economic valuation methods used by studies with the highest impact on scientific research was conducted. The findings suggest that while studies provide valuable data and insights, their practical applicability in policymaking is limited, due to contextual relevance and bias issues. Overall, the review underscores the need for a paradigm shift to better inform real-world policy decisions, identifying the Marine Spatial Planning (MSP) process as a key framework for bridging these gaps in future research and policy implementation.
Impact of wave–current coupling on the bottom boundary layer in Bohai Bay
Authors: Liu Siyu , Li Guangxue , Liu Xue , Qiao Lulu , Wang Nan , Liu Shidong , Wang Xiangdong , Yu Di , Zhang Lei
Published: January 2025

Sediment resuspension primarily occurs within the bottom boundary layer (BBL) of water bodies, particularly in silty coastal environments, and helps form the fluid mud layer (FML). In this study, we report data on the water level, waves, currents, and suspended sediment concentration (SSC) collected from the Bohai Sea over one year, at a vertical resolution of 4 cm, by using the acoustic wave and current profiler, acoustic Doppler current profiler, and an acoustic backscattering system. The aim was to investigate the mechanisms of formation and disappearance of the FML as driven by wave–current interactions on silty seabeds The findings revealed a thin and stable FML within 4–12 cm of the seabed in shallow waters. Strong waves contributed more significantly to sediment resuspension than strong currents. Moreover, the SSC near the seabed was generally governed by the currents, while waves were predominant in this regard in stormy conditions. The index of intensity of the bottom shear β—defined as the ratio of the wave–current-induced shear stress to the critical shear stress—was identified as a sensitive indicator of variations in the SSC. Significant sediment resuspension occurred in case of the coupling of large wave and current events on the fine-grained seabed, when the value of β exceeded 10 for more than 20 h and the Rouse number persisted below 0.01 for over 30 h. Following the storm (β < 10), the sediment gradually accumulated, and this led to the formation of the FML. Prolonged periods in which the value of β surpassed 10 for over 10 h while that of the Rouse number remained below 0.01 for more than 30 h resulted in considerable sediment resuspension and the destruction of the FML. Our results highlight the profound impacts of wave–current interactions on the formation and disappearance of the FML within the BBL in silty, shallow marine environments. The work here offers critical insights into the dynamics of fine-particle sediment, and provides suggestions for mitigating the negative effects associated with the FML.
Association between albumin changes and prognosis in older patients with acute myocardial infarction
Authors: Yang Zhi-cheng , Zhang Lei , Xi Ying-bin , Jiang Gui-hua , Lin He , Pan Hui , Wang Zhi-hao
Published: January 2025

Background and aims

Acute myocardial infarction (AMI) is the leading cause of death in the world. Therefore, early identification of the prognosis of older patients with AMI are particularly urgent, and better to improve treatment. This study aimed to explore the association between albumin changes and prognosis in older patients with AMI.

Methods

Outcomes included all-cause death during hospitalization, cardiac death, gastrointestinal hemorrhage, nonfatal myocardial infarction, acute heart failure, and severe arrhythmia. Multivariable-adjusted Cox regression analysis and curve fitting were used to assess the relationship between albumin changes and prognosis in patients with AMI.

Results

Our study included 288 older patients with AMI. A S-shaped association between the albumin changes and mortality of patients with AMI was found. For all-cause death, we found two inflection points were − 3.27 and 0.92 g/L. On the left side of −3.27 g/L, the OR was 0.41 (OR: 0.41, 95%CI: 0.32–0.64, p < 0.05). On the right side of 0.92 g/L, the OR was 0.58 (OR: 0.58, 95%CI: 0.34–0.86, p < 0.05). The OR was 2.12 between −3.27 and 0.92 g/L (OR: 2.12, 95%CI: 1.16–6.24, p < 0.05). For cardiac death, two inflection points were − 3.19 and 1.17 g/L. On the left side of −3.19 g/L, the OR was 0.45 (OR: 0.45, 95%CI: 0.28–0.79, p < 0.05). On the right side of 1.17 g/L, the OR was 0.63 (OR: 0.63, 95%CI: 0.38–0.86, p < 0.05). The OR was 4.53 between −3.19 and 1.17 g/L (OR: 4.53, 95%CI: 0.90–12.52, p > 0.05). After adjusting for all potential covariates, albumin changes were negatively associated with gastrointestinal hemorrhage (OR: 0.87; 95%CI: 0.81–0.94, p < 0.001). After adjusting for all potential covariates, albumin changes were negatively associated with acute heart failure (OR: 0.86; 95%CI: 0.75–0.99, p = 0.046).

Conclusion

Out findings showed that a S-shaped association between the albumin changes and mortality of older AMI patients, with the inflection of roughly −3.27 g/L and 0.92/L. And changes in albumin levels are negatively correlated with gastrointestinal bleeding and acute heart failure. These findings were helpful for the clinical treatment.
Octodon degus laboratory colony management principles and methods for behavioral analysis for Alzheimer’s disease neuroscience research
Authors: Garduño B. Maximiliano , Holmes Todd C. , Deacon Robert M. J. , Xu Xiangmin , Cogram Patricia
Published: January 2025

The Chilean degu (Octodon degus) is a medium sized, long-lived rodent with traits that make them a natural model for neuroscience research. Their social behaviors, diurnality, and extended developmental time course, when compared to other rodents, make them useful for social behavioral, chronobiology, and developmental research. Lab-kept degus have a long lifespan (5–8 years) and may naturally develop age-related diseases that resemble Alzheimer’s disease. While there is significant interest in using the Octodon degus for neuroscience research, including aging and Alzheimer’s disease studies, laboratory management and methods for degus research are currently not standardized. This lack of standardization potentially impacts study reproducibility and makes it difficult to compare results between different laboratories. Degus require species-specific housing and handling methods that reflect their ecology, life history, and group-living characteristics. Here we introduce major principles and ethological considerations of colony management and husbandry. We provide clear instructions on laboratory practices necessary for maintaining a healthy and robust colony of degus for Alzheimer’s disease neuroscience research towards conducting reproducible studies. We also report detailed procedures and methodical information for degu Apoe genotyping and ethologically relevant burrowing behavioral tasks in laboratory settings.
Chinese medicine for headaches in emergency department: a retrospective analysis of real-world electronic medical records
Authors: Mao Zhenhui , Wu Shirong , Fan Yuzhen , Sun Jingbo , Lyu Shaohua , Su Qiaozhen
Published: January 2025

Background

Headaches are common complaints in the emergency department (ED) and have raised concern about acute medication overuse. Chinese medicine is a major complementary and alternative medicine in China and effective for headaches. This study aims to summarize characteristics of headache patients at EDs and the utilization of Chinese medicine for headache managements in EDs.

Methods

The study conducted a retrospective analysis based on existing electronic medical records at EDs from four branches of Guangdong Provincial Hospital of Chinese Medicine. Only complete medical records with a first diagnosis of headache within the specified timeframe were included. Data was extracted, screened and standardized using a structured approach. Descriptive analyses and Apriori algorithm-based association rules were employed for the study.

Results

A total of 3,355 medical records were analyzed, with over 86% of headaches classified as non-urgent. Approximately 97% of the patients received a general diagnosis of headaches without further classification. Hypertension was the most prevalent concomitant diagnosis, affecting 27.42% of the patients. Western medicine was prescribed to 66% of the patients for headaches and co-existing conditions, while each type of acute medication was prescribed to fewer than 10% of the patients. Conversely, over one-third of the patients utilized headache-specific patented Chinese herbal medicine products. Additionally, oral and topical Chinese herbal medicine treatments were also administered for headaches in the emergency departments.

Conclusion

The majority of headaches consulting in the EDs were non-urgent and treated with various forms of Chinese medicine, alone or in conjunction of western medicine. Chinese herbal medicine may be promoted as alternatives to Western acute medications for treating benign headaches.
EURAD State-of-the-Art Report: Assessment of the chemical evolution at the disposal cell scale – part II – gaining insights into the geochemical evolution
Authors: Deissmann Guido , Neeft Erika , Jacques Diederik
Published: January 2025

Long time frames are to be considered in the safety and performance assessment of deep geological disposal of intermediate and high level radioactive waste. Geochemical conditions will change in the waste, conditioning matrix, waste package, engineered barriers and the host rock–all components present at the disposal cell scale. This aspect of geological disposal was the focus of the work package ACED (Assessment of chemical evolution of intermediate level (ILW) and high level (HLW) waste at disposal cell scale) in the EURAD project (the European Joint Programme on Radioactive Waste Management). The first part of this review provided a narrative of the geochemical evolution of the disposal cell. In this second part, an overview is given about methods and approaches that can be used to gain further insights into the processes driving the geochemical evolution, more in particular (i) laboratory and in-situ experiments, (ii) archaeological and natural analogues, and (iii) modelling tools. The review concludes with a short discussion on the consequences on material properties, waste forms and radionuclide mobility.
Relationship between sleep quality and dietary nutrients in rural elderly individuals: a latent class analysis
Authors: Zhao Xinlei , Xie Xinyan , Zhou Han , Zhong Feng , Liu Cuiping
Published: January 2025

Background

This study sought to identify sleep patterns in older adults residing in rural regions, as well as investigate the potential influence of dietary nutrient intake on these patterns.

Methods

Data were collected from a cross-sectional sample of Qingdao Town, Shandong Province, China. The study investigated 1,167 elderly participants using a general questionnaire, the Pittsburgh Sleep Quality Index, the simplified Food Frequency Questionnaire, and 24-h dietary recall methods. Latent profile analysis and binary logistic regression were applied for data analysis.

Results

Two sleep categories were identified as “Quick falling asleep, long time, high efficiency group,” Class 1(89.1%) and “Difficult falling asleep, short time, low efficiency group,” Class 2(10.9%). In comparison to Class 1, individuals in Class 2 exhibited a higher likelihood of experiencing difficulties in falling asleep quickly and having poor sleep efficiency when consuming less than 50 g/d of potatoes (OR = 1.863, p = 0.034). Conversely, a daily protein intake of 60 g or more (OR = 0.367, p = 0.007), a daily intake of retinol of 700 equivalents or more (OR = 0.212, p = 0.002), and a daily milk intake of 300 g or more (OR = 0.295, p = 0.035) were associated with a greater probability of falling asleep quickly, having longer sleep duration, and experiencing higher sleep efficiency.

Conclusion

Our analysis identified two distinct sleep quality patterns among elderly individuals in rural areas. The sleep quality of rural elderly individuals is influenced by their dietary habits. The findings demonstrated a positive association between enhanced sleep quality and higher intake of dairy products, potatoes, and foods containing retinol and protein. Therefore, we propose increased consumption of these nutritional sources for the elderly population.
Hippocampus-sparing volume-modulated arc therapy in patients with World Health Organization grade II glioma: a feasibility study
Authors: Xie Renxian , Huang Hongxin , Cai Qingxin , Lu Jiayang , Chen Tong , Xie Keyan , Chen Jianzhou , Chen Chuangzhen
Published: January 2025

Background

Radiotherapy can improve the survival rates of patients with glioma; meanwhile, impaired cognitive functions have been brought to the forefront with the offending organ, the radiosensitive hippocampus. This study aimed to assess the feasibility of hippocampus-sparing volumetric-modulated arc therapy (HS VMAT) in patients with World Health Organization (WHO) grade II glioma.

Methods

HS VMAT plans and non-hippocampus-sparing volumetric-modulated arc therapy (NHS VMAT) plans were generated using a computed tomography (CT) dataset of 10 patients who underwent postoperative radiotherapy. The dose volume histogram (DVH), homogeneity index (HI), conformity index (CI), and irradiated dose of the hippocampus and other organs at risk (OARs) were analyzed.

Results

No significant differences were observed in HI and CI between the two plans. Regarding the protection of OARs, HS VMAT plans were equally capable and even lowered the radiation dosages to the brainstem (35.56 vs. 41.74 Gy, p = 0.017) and spinal cord (1.34 vs. 1.43 Gy, p = 0.006). Notably, HS VMAT plans markedly decreased doses to the ipsilateral hippocampus and the contralateral hippocampus, demonstrating its efficacy in hippocampal dose reduction.

Conclusion

The HS VMAT plan can be used to efficiently lower the dosage delivered to the hippocampus and may, to some extent, help lessen the risk of cognitive damage. The encouraging results of our study need to be further validated by clinical trials to confirm the benefits of the HS VMAT plans in preserving cognitive functions in patients with glioma.
Unveiling DNA methylation: early diagnosis, risk assessment, and therapy for endometrial cancer
Authors: Li Minzhen , Xia Zhili , Wang Ruiyu , Xi Mingrong , Hou Minmin
Published: January 2025

Endometrial cancer (EC), one of the most common gynecologic malignancies worldwide, poses a significant burden particularly among young women, with poor treatment outcomes and prognosis for advanced and recurrent patients. Epigenetic changes, encompassing DNA methylation, are involved in the occurrence and progression of tumors and hold promise as effective tools for screening, early diagnosis, treatment strategy, efficacy evaluation, and prognosis analysis. This review provides a comprehensive summary of DNA methylation-based early diagnostic biomarkers in EC, with a focus on recent valuable research findings published in the past two years. The discussion is organized according to sample sources, including cervical scraping, vaginal fluid, urine, blood, and tissue. Additionally, we outline the role of DNA methylation in EC risk assessment, such as carcinogenesis risk, feasibility of fertility preservation approaches, and overall prognosis, aiming to provide personalized treatment decisions for patients. Finally, we review researches on DNA methylation in resistance to first-line treatment of EC and the development of new drugs, and envision the future applications of DNA methylation in EC.