bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipid Production in Streptomyces jeddahensis Is Enhanced by Glucose and Fatty Acid Derivatives, with Temperature Variations Influencing Gene Expression and Biosynthesis
Authors: Apriliana Pamella , Kahar Prihardi , Rachmadona Nova , Restu Witta Kartika , Kondo Akihiko , Ogino Chiaki
Published: January 2025

The potential of Streptomyces jeddahensis for lipid production was investigated, focusing on its ability to utilize various carbon sources under different temperature conditions. Glucose and fatty acid derivatives (sodium hexanoate, sodium octanoate, and sodium decanoate) were tested at 30 °C, 37 °C, and 45 °C to optimize growth and lipid biosynthesis. The results revealed that 37 °C was the optimal temperature for lipid accumulation, with sodium octanoate leading to the highest lipid content. While growth declined at 45 °C, significant lipid production persisted, indicating an adaptive stress response. Gene expression analysis identified upregulation of key metabolic genes, including STSP_03080 (DGAT) and STSP_67970 (fabD), under thermal stress, highlighting enhanced fatty acid biosynthesis pathways. This study demonstrates the capacity of S. jeddahensis to efficiently convert medium-chain fatty acids into lipids, supporting its use as a sustainable and scalable platform for biodiesel production and other lipid-based industrial applications.
Survey on Secure Scientific Workflow Scheduling in Cloud Environments
Authors: Saeed Hadeel Amjed , Al-Janabi Sufyan T. Faraj , Yassen Esam Taha , Aldhaibani Omar A.
Published: January 2025

In cloud computing environments, the representation and management of data through workflows are crucial to ensuring efficient processing. This paper focuses on securing scientific workflow scheduling, which involves executing complex data-processing tasks with specific dependencies. The security of intermediate data, often transmitted between virtual machines during workflow execution, is critical for maintaining the integrity and confidentiality of scientific workflows. This review analyzes methods for securing scientific workflow scheduling in cloud environments, emphasizing the application of security principles such as confidentiality, authentication, and integrity. Various scheduling algorithms, including heuristics and metaheuristics, are examined for their effectiveness in balancing security with constraints like execution time and cost.
Enhancing Human–Agent Interaction via Artificial Agents That Speculate About the Future
Authors: Bennett Casey C. , Bae Young-Ho , Yoon Jun-Hyung , Kim Say Young , Weiss Benjamin
Published: January 2025

Human communication in daily life entails not only talking about what we are currently doing or will do, but also speculating about future possibilities that may (or may not) occur, i.e., “anticipatory speech”. Such conversations are central to social cooperation and social cohesion in humans. This suggests that such capabilities may also be critical for developing improved speech systems for artificial agents, e.g., human–agent interaction (HAI) and human–robot interaction (HRI). However, to do so successfully, it is imperative that we understand how anticipatory speech may affect the behavior of human users and, subsequently, the behavior of the agent/robot. Moreover, it is possible that such effects may vary across cultures and languages. To that end, we conducted an experiment where a human and autonomous 3D virtual avatar interacted in a cooperative gameplay environment. The experiment included 40 participants, comparing different languages (20 English, 20 Korean), where the artificial agent had anticipatory speech either enabled or disabled. The results showed that anticipatory speech significantly altered the speech patterns and turn-taking behavior of both the human and the agent, but those effects varied depending on the language spoken. We discuss how the use of such novel communication forms holds potential for enhancing HAI/HRI, as well as the development of mixed reality and virtual reality interactive systems for human users.
Enhancement of Dough Processing and Steamed Bread Quality with Modified Soybean Residue Dietary Fiber
Authors: Zhao Jun , Xie Wenlong , Chen Zhilong , Zheng Yuqian , Li Sheng
Published: January 2025

The effects of different modified soybean residues’ dietary fiber on the physicochemical properties of wheat dough and the quality of steamed bread were systematically analyzed in this study. The physical and chemical parameters of dough, such as texture characteristics, water distribution, secondary structure, and the specific volume, color, and sensory evaluation results of steamed bread products were analyzed in detail. The results showed that adding 6% modified soybean residue dietary fiber enhanced the gluten network, increasing the S–S bond content and improving gluten stability. Notably, the inclusion of 6% residue modified by the ultrasound combined with enzyme method (UEDF) led to a 2.55% increase in the β-fold content of gluten proteins and a 3.60% rise in disulfide bond content. These changes resulted in a reduction in dough relaxation time, promoting a more uniform and compact pore structure in the dough. Additionally, steamed bread made with 6% UEDF showed a 0.3 mL/g increase in specific volume, a 4.69 point rise in L* value, and improved sensory attributes such as taste, odor, and appearance. These research results provide valuable insights and guidance for the development of soybean residue dietary fiber foods.
Reasons Behind (Un)Healthy Eating Among School-Age Children in Southern Peru
Authors: Eduardo Karina , Velásquez José Carlos , Mayta-Hancco Jhony , Rios-Mera Juan D. , Lozada-Urbano Michelle , Saldaña Erick
Published: January 2025

In recent decades, rates of childhood overweight and obesity have increased worldwide, surpassing those of adults. Understanding the factors influencing children’s food choices is essential to promote healthy eating habits. This study examined why school-aged children make healthy and unhealthy food choices and how parents’ eating habits influence their children’s choices. Children’s ability to rank 25 food pictures according to their healthfulness was assessed using a free sorting task (FST), while parents made a free list of healthy and unhealthy foods, and the cognitive salience index (CSI) was calculated. Children were grouped into two groups based on their responses: one mainly from Moquegua and the other from Puno. In general, children from Moquegua demonstrated a greater ability to identify healthy foods than those from Puno. The CSI provided information on healthy and unhealthy foods in each region. These findings underline the potential of selecting palatable and healthy foods to improve children’s diets in the short term and contribute to the development of healthier products in the food industry. In addition, studies in other regions of Peru are recommended to understand children’s perceptions better.
Unravelling the Relationship Between Height, Lean Mass, Alzheimer’s Disease and Cognition Through Mendelian Randomization
Authors: Huang Jingxian , Zhang Linxuan , Bodimeade Christopher , Nassan Malik , Gill Dipender , Cronjé Héléne T. , Dib Marie-Joe , Daghlas Iyas
Published: January 2025

Background: Genetic evidence from Mendelian randomization (MR) analyses suggests that higher lean mass causally protects against Alzheimer’s disease (AD) and enhances cognitive function. However, the potential confounding role of height, which shares genetic etiology with lean mass, has not been fully examined. Methods: Genetic predictors of whole-body lean mass were obtained from a genome-wide association study (GWAS) performed in the UK Biobank cohort (UKB; n = 448,322). Genetic predictors of height were also obtained from UKB (height0.5M = 455,332) and from a GWAS meta-analysis (height1.5Mn = 1,578,425). The study outcomes included clinically diagnosed AD (21,982 cases and 41,944 controls) and cognitive performance (n = 269,867). All study participants were of European ancestry. We conducted univariable and multivariable MR analyses to examine the total and independent effects of lean mass and height on the specified outcomes under different statistical adjustment strategies. Results: In univariable MR analyses, genetically proxied lean mass (odds ratio [OR] per 1-standard deviation [SD] increase 0.81, 95% confidence interval [CI] 0.72–0.91, p = 3.8 × 10−4) and height (OR 0.90, 95% CI 0.84–0.96, p = 0.001) were associated with reduced risk of AD. Genetically proxied lean mass (β 0.10, 95% CI 0.08–0.12, p = 6.24 × 10−6) and height (β 0.07, 95% CI 0.05–0.08, p = 1.16 × 10−15) were further associated with improved cognitive performance. In multivariable MR analyses, adjustment for height1.5M partially attenuated the lean mass association with AD (OR 0.91, 95% CI 0.74–1.12, p = 0.40), whereas the height1.5M-AD association remained similar after adjusting for lean mass (OR 0.89, 95% CI 0.79–1.00, p = 0.04). Adjustment for height also attenuated the association of lean mass with cognitive performance (β 0.00, 95% CI −0.07–0.06, p = 0.94), whereas height maintained a similar association with improved cognitive performance after adjustment for lean mass (β 0.07, 95% CI 0.03–0.10, p = 0.001). Conclusions: Height may confound the genetic associations between lean mass and both cognitive performance and AD risk. Residual direct effects of lean mass on AD risk cannot be excluded due to limitations in statistical power and genetic instrument strength in MVMR. These findings emphasize the necessity of adjusting for height when using MR to investigate the clinical effects of lean mass.
Paediatric Preparedness: Document Analysis of the Challenges Experienced Using Smartwatch Technologies to Support Children Living with a Chronic Health Condition
Authors: Butler Sonia , Sculley Dean , Santos Derek , Gironès Xavier , Singh-Grewal Davinder , Coda Andrea
Published: January 2025

Smartwatch technology is increasingly being used to support the management of chronic health conditions. Yet, many new digital health innovations fail because the correct foundations are not well established. This exploratory study aims to uncover the challenges experienced during the setup phase of a smartwatch intervention, to support the prototype development of a digital health intervention for children. Five children with a chronic health condition were asked to wear a smartwatch for 14 days that collects health data (pain levels, medication adherence, and physical activity performance). To explore the experiences of these children, their parents and the research team, all written records were analysed using READ’s four steps of document analysis and reported using the Standards for Reporting Qualitative Research checklist. The following three themes emerged: 1.) Infrastructure limitations: inexpensive smartphones prevented connection, and outpatient clinics’ internet black spots constrained setup and training; 2.) Personal phone restrictions: limited setup, training, and engagement; 3.) Elimination of the parent’s phone: provided children with digital support (a smartphone, pre-installed apps, cellular data) to allow active participation. Overall, we identified barriers hindering the use of smartwatch technology in clinical practice. More resources are needed to ensure paediatric preparedness for digital health support.
Gynaecological Health Patterns and Motherhood Experiences of Female Professional Football Players
Authors: Ramagole Dimakatso Althea , van Rensburg Dina Christa Janse , Cowie Charlotte , Mehta Ritan , Ramkilawon Gopika , Pluim Babette M. , Kerkhoffs Gino , Gouttebarge Vincent
Published: January 2025

The aim of this paper is to explore the gynaecological health patterns, contraceptive use, body perception, and motherhood experiences of female professional football players. The participants were recruited via email using FIFPRO (Football Players Worldwide). Online questionnaires were completed by consenting participants. The mean age at menarche was 13.5 years with an average cycle length of 26 days, and a bleeding period of 5 days. Cycle irregularities were experienced by 30%, and menstrual symptoms by 74%. Half of the participants used contraceptives, 60% using hormonal contraceptives, primarily oral contraceptive pills (38%), followed by implants (20%). The body satisfaction score was normal but there was a high drive-for-thinness (DT) score. The motherhood rate was low (1%), with participants experiencing normal conception, vaginal delivery, return to training after 6 weeks, and return to competition after 12 weeks. Our findings are consistent with findings in other elite female athletes with cycle irregularities and a significant number of cycle-related symptoms. The majority of those using contraceptives preferred hormonal contraceptives, reflecting trends seen in other elite athletes. While body satisfaction scores were normal, there was a high DT score, similar to that observed in lean and weight-category sports. The motherhood rate was low, consistent with previous findings in professional football players and other elite athletes. This may be due to a lack of financial support during pregnancy and the post-partum period. FIFPRO and its affiliated unions are negotiating better contracts for female football players.
Effect of the COVID-19 Pandemic on Suicide Mortality in Brazil: An Interrupted Time Series Analysis
Authors: Meira Karina Cardoso , Guimarães Raphael Mendonça , Jomar Rafael Tavares , Silva Cosme Marcelo Furtado Passos da , Braiti Fabiana Serpa , Dantas Eder Samuel Oliveira
Published: January 2025

This study analyzed the effect of the COVID-19 pandemic on suicide rates among Brazilian residents, stratified by sex. It examined monthly suicide rates using interrupted time series analysis. Researchers compared the months before the pandemic (January 2017 to February 2020) with those after the first diagnosed case of COVID-19 in Brazil (March 2020 to December 2023). They applied an interrupted time series model (quasi-Poisson) to account for serial autocorrelation in the residuals and seasonality. During this period, authorities reported 102,081 suicides in Brazil. The age-standardized annual suicide rate among men was 3.71 times higher than the rate among women (12.33 suicides per 100,000 vs. 3.32 suicides per 100,000 women). The South and Midwest regions had the highest standardized annual average suicide rates. Suicide rates among men dropped abruptly at the pandemic’s onset (RR < 1, p < 0.05). However, Black men, women aged 15 to 19 years, and elderly individuals exhibited a significant increase (RR > 1, p < 0.05). Over time, suicide rates rose across most age groups, regions, and methods studied (RR > 1, p < 0.05). The pandemic’s impact differed significantly between men and women based on stratification variables. Nonetheless, a progressive upward trend emerged throughout the pandemic.
A High-Resolution Crystallographic Study of Cytochrome c6: Structural Basis for Electron Transfer in Cyanobacterial Photosynthesis
Authors: Zhang Botao , Xu Yuancong , Liu Shuwen , Chen Sixu , Zhao Wencong , Li Zhaoyang , Wang Junshuai , Zhao Weijian , Zhang Heng , Dong Yuhui , Gong Yong , Sheng Wang , Cao Peng
Published: January 2025

Cyanobacterial cytochrome c6 (Cyt c6) is crucial for electron transfer between the cytochrome b6f complex and photosystem I (PSI), playing a key role in photosynthesis and enhancing adaptation to extreme environments. This study investigates the high-resolution crystal structures of Cyt c6 from Synechococcus elongatus PCC 7942 and Synechocystis PCC 6803, focusing on its dimerization mechanisms and functional implications for photosynthesis. Cyt c6 was expressed in Escherichia coli using a dual-plasmid co-expression system and characterized in both oxidized and reduced states. X-ray crystallography revealed three distinct crystal forms, with asymmetric units containing 2, 4, or 12 molecules, all of which consist of repeating dimeric structures. Structural comparisons across species indicated that dimerization predominantly occurs through hydrophobic interactions within a conserved motif around the heme crevice, despite notable variations in dimer positioning. We propose that the dimerization of Cyt c6 enhances structural stability, optimizes electron transfer kinetics, and protects the protein from oxidative damage. Furthermore, we used AlphaFold3 to predict the structure of the PSI-Cyt c6 complex, revealing specific interactions that may facilitate efficient electron transfer. These findings provide new insights into the functional role of Cyt c6 dimerization and its contribution to improving cyanobacterial photosynthetic electron transport.
Repetitive Transcranial Magnetic Stimulation for the Treatment of Spinal Cord Injury: Current Status and Perspective
Authors: Fan Shu , Wang Wei , Zheng Xiaolong
Published: January 2025

Spinal cord injury (SCI) can lead to devastating dysfunctions and complications, significantly impacting patients’ quality of life and aggravating the burden of disease. Since the main pathological mechanism of SCI is the disruption of neuronal circuits, the primary therapeutic strategy for SCI involves reconstructing and activating circuits to restore neural signal transmission. Repetitive transcranial magnetic stimulation (rTMS), a noninvasive brain stimulation technique, can modulate the function or state of the nervous system by pulsed magnetic fields. Here, we discuss the basic principles and potential mechanisms of rTMS for treating SCI, including promoting the reconstruction of damaged circuits in the spinal cord, activating reorganization of the cerebral cortex and circuits, modulating the balance of inputs to motoneurons, improving the microenvironment and intrinsic regeneration ability in SCI. Based on these mechanisms, we provide an overview of the therapeutic effects of rTMS in SCI patients with motor dysfunction, spasticity and neuropathic pain. We also discuss the challenges and prospectives of rTMS, especially the potential of combination therapy of rTMS and neural progenitor cell transplantation, and the synergistic effects on promoting regeneration, relay formation and functional connectivity. This review is expected to offer a relatively comprehensive understanding and new perspectives of rTMS for SCI treatment.
Biomarkers of Intrathecal Synthesis May Be Associated with Cognitive Impairment at MS Diagnosis
Authors: Virgilio Eleonora , Ciampana Valentina , Puricelli Chiara , Naldi Paola , Bianchi Angelo , Dianzani Umberto , Vecchio Domizia , Comi Cristoforo
Published: January 2025

The pathophysiology of cognitive impairment (CI) in multiple sclerosis (MS) remains unclear. Meningeal B cell aggregates may contribute to cortical grey matter pathology. Cerebrospinal fluid (CSF), kappa free light chains (KFLC), and KFLCs-Index (kappa-Index) are reliable quantitative markers of intrathecal synthesis, but few data have been presented exploring the association with CI, and no data are present for lambda FLC (LFLC) in MS. We evaluated cognition using the Brief International Cognitive Assessment for MS (BICAMS) battery and collected serum and CSF at diagnosis in newly diagnosed drug-naïve MS patients. We observed that patients with impaired verbal memory and overall CI showed increased CSF KFLCs (respectively p: 0.0003 and p: 0.003) and kappa-Index (respectively p: 0.01 and p: 0.02) compared to those with normal verbal memory and no CI. Patients with CI also displayed lower CSF LFLCs (p: 0.04) and lambda-Index (p: 0.001); however, only CSF KFLC negatively correlated with normalized results of verbal memory (for age, sex, and educational levels), even after correction for EDSS (r: −0.27 p: 0.01). Finally, CSF FKLC and kappa-Index were significant predictors of verbal memory in a multivariate analysis. Our results, suggest that intrathecal B cell activity might contribute to CI development in MS patients.
The Importance of Edible Medicinal Mushrooms and Their Potential Use as Therapeutic Agents Against Insulin Resistance
Authors: Németh Zsuzsanna , Paulinné Bukovics Mariann , Sümegi Liza Dalma , Sturm Gábor , Takács István , Simon-Szabó Laura
Published: January 2025

In addition to conventional treatments, there is growing interest in preventive and complementary therapies. Proper nutrition can prevent the manifestation of several chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer, and can attenuate the severity of these diseases. Edible mushrooms have been used as nutrition and medicine for thousands of years. The spectrum and quantity of their medicinal compounds made them a widely investigated target both in basic research and clinical trials. The most abundant and medically important components are polysaccharides, terpenoids, phenols, and heterocyclic amines, but bioactive proteins, vitamins, including vitamin D, polyunsaturated fatty acids, and essential minerals are also important ingredients with noteworthy health benefits. Mushroom extracts have anti-diabetic, anti-hyperlipidemic, anti-inflammatory, antioxidant, cardioprotective, anti-osteoporotic, and anti-tumor effects and are well tolerated, even by cancer patients. In our previous review we detailed the molecular aspects of the development of type 2 diabetes, discussing the role of physical activity and diet, but we did not detail the role of medicinal mushrooms as part of nutrition. In this review, we aimed to summarize the most important medical mushrooms, along with their natural habitats, growing conditions, and components, that are presumably sufficient for the prevention and treatment of insulin resistance.
Comparative Transcriptomic Analysis Reveals New Insights into Spawn Aging in Agaricus bisporus: Mitochondrial Dysfunction
Authors: Shu Lili , Zeng Zhiheng , Chen Meiyuan , Zhao Jiazhi , Zhang Xiaoyan , Dai Jianqing , Cai Zhixin , Lu Yuanping , Qiu Zhiheng , Zeng Hui
Published: January 2025

Spawn aging poses a substantial challenge to the Agaricus bisporus industry. This study focuses on the role of mitochondrial dysfunction in the aging process of A. bisporus spawn. We conducted a comprehensive comparative transcriptome analysis to elucidate the molecular mechanisms underlying A. bisporus spawn aging. A total of 1620 genes with significant expression changes between the normal and aged spawn were identified, including 917 up-regulated genes and 703 down-regulated genes. Our results revealed a notable down-regulation of genes involved in carbohydrate metabolism, mitochondrial energy metabolism, reactive oxygen species (ROS) scavenging, repair mechanisms for oxidative stress-induced damage, fatty acid β-oxidation, and amino acid degradation in aged A. bisporus spawn. Additionally, we observed a decreased expression of genes involved in critical signal transduction pathways associated with mitochondrial function in aged mycelium as well as genes responsible for maintaining mitochondrial stability. The up-regulated genes in aged spawn mainly affect mitochondrial fission and programmed cell death, impacting mitochondrial function. Overall, the present study first provides evidence for the pivotal role of mitochondrial dysfunction in the aging process of A. bisporus spawn and contributes to the development of targeted strategies to enhance mitochondrial function, mitigate spawn aging, and improve the yield and quality of A. bisporus cultivation.
Aspirin Inhibits the In Vitro Adipogenic Differentiation of Human Adipose Tissue-Derived Stem Cells in a Dose-Dependent Manner
Authors: Funke Sarah , Wiggenhauser Paul Severin , Grundmeier Anna , Fuchs Benedikt , Koban Konstantin , Demmer Wolfram , Giunta Riccardo E. , Kuhlmann Constanze
Published: January 2025

Aspirin (ASA) is one of the most used medications worldwide and has shown various effects on cellular processes, including stem cell differentiation. However, the effect of ASA on adipogenesis of adipose tissue-derived stem cells (ASCs) remains largely unknown. Considering the potential application of ASCs in regenerative medicine and cell-based therapies, this study investigates the effects of ASA on adipogenic differentiation in human ASCs. ASCs were exposed to varying concentrations of ASA (0 µM, 400 µM, and 1000 µM) and evaluated for changes in morphology, migration, and adipogenic differentiation. While ASA exposure did not affect self-renewal potential, migration ability, or cell morphology, it significantly reduced lipid vacuole formation at 1000 µM after 21 days of adipogenic differentiation (p = 0.0025). This visible inhibition correlated with decreased expression of adipogenic markers (PPARG, ADIPOQ, and FABP4) and the proliferation marker MKi67 under ASA exposure in comparison to the control (ns). Overall, the findings demonstrate that ASA inhibits adipogenic differentiation of human ASCs in a dose-dependent manner in vitro, contrasting its known role in promoting osteogenic differentiation. This research highlights ASA’s complex effects on ASCs and emphasizes the need for further investigation into its mechanisms and potential therapeutic applications in obesity and metabolic diseases. The inhibitory effects of ASA on adipogenesis should be considered in cell-based therapies using ASCs.
Antiplasmodial Activity of a New Chemotype of Croton sylvaticus Hochst. Ex C. Krauss Essential Oil
Authors: Taguimjeu Pierre Leonel K. Tafokeu , Fongang Yannick Stéphane Fotsing , Genva Manon , Shinyuy Lahngong Methodius , Held Jana , Frederich Michel , Ngouela Silvère Augustin , Fauconnier Marie-Laure
Published: January 2025

Croton sylvaticus, a tropical African plant, is traditionally used to treat several diseases, including fever, inflammation, and malaria. Essential oils (EOs) from the plant’s leaves, roots, and trunk bark were obtained by hydrodistillation, and their chemical composition was analyzed by gas chromatography–mass spectrometry (GC-MS). The major constituents identified were virdiflorene (18.13 ± 0.46%) in root EO, (E)-β-caryophyllene (18.40 ± 0.60%) in trunk bark EO, and farnesyl acetone (15.26 ± 0.25%) in leaf EO. Notably, Cameroonian C. sylvaticus leaf EO exhibited a distinct and newly described chemotype with high levels of farnesyl acetone, β-copaene-4-α-ol, β-cadinene, α-humulene, and trans-longipinocarveol. In vitro testing revealed significant antiplasmodial activity against Plasmodium falciparum asexual (Pf3D7) and sexual (NF-54 strain) stages, with trunk bark EO showing the highest potency (IC50: 9.06 ± 2.15 µg/mL for Pf3D7 and 0.56 µg/mL for gametocytes). These findings support the traditional antimalarial use of C. sylvaticus and represent the first chemical profile and antiplasmodial efficacy report for its root and trunk bark EOs against both parasite stages. To the best of our knowledge, we also report for the first time the antiplasmodial activity of an EO that exerts significant activity against both the asexual and sexual forms of P. falciparum.
Exploring How Adipose Tissue, Obesity, and Gender Influence the Immune Response to Vaccines: A Comprehensive Narrative Review
Authors: De Sanctis Juan Bautista , Balda Noria Germán , García Alexis Hipólito
Published: January 2025

Vaccines represent an essential tool for the prevention of infectious diseases. Upon administration, a complex interaction occurs between the vaccine formulation and the recipient’s immune system, ultimately resulting in protection against disease. Significant variability exists in individual and population responses to vaccination, and these differences remain the focus of the ongoing research. Notably, well-documented factors, such as age, gender, and genetic predisposition, influence immune responses. In contrast, the effects of overweight and obesity have not been as thoroughly investigated. The evidence indicates that a high body mass index (BMI) constitutes a significant risk factor for infections in general, with adipose tissue playing a crucial role in modulating the immune response. Furthermore, suboptimal levels of vaccine seroconversion have been observed among individuals with obesity. This review provides a plausible examination of the immunity and protection conferred by various vaccines in individuals with an overweight status, offering a comprehensive analysis of the mechanisms to enhance vaccination efficiency.
Blood–Brain Barrier Disruption in Schizophrenia: Insights, Mechanisms, and Future Directions
Authors: Zhang Fangsong , Zhang Jianye , Wang Xuexue , Han Mengyang , Fei Yi , Wang Jinhong
Published: January 2025

The blood–brain barrier (BBB) plays a crucial role in maintaining the homeostasis of the central nervous system by regulating solute transport and preventing neurotoxic substances from infiltrating brain tissue. In schizophrenia, emerging evidence identifies BBB dysfunction as a key pathophysiological factor associated with neuroinflammation, tight junction abnormalities, and endothelial dysfunction. Recent advancements in neuroimaging techniques, such as arterial spin labeling (ASL), have provided valuable tools for investigating BBB permeability and its role in disease progression. This review synthesizes findings from postmortem studies, serum and cerebrospinal fluid biomarker analyses, and advanced neuroimaging research to elucidate BBB alterations in schizophrenia. It highlights the mechanistic roles of tight junction protein dysregulation, neurovascular unit dysfunction, and immune responses in disrupting BBB integrity. Furthermore, the review examines the bidirectional effects of antipsychotic medications on BBB, addressing both therapeutic opportunities and potential challenges. By emphasizing the pivotal role of BBB dysfunction in schizophrenia pathogenesis, this review underscores its translational potential. Through the integration of multidisciplinary evidence, it lays the foundation for innovative diagnostic approaches and therapeutic strategies, enhancing our understanding of schizophrenia’s complex pathophysiology.
Role of GmFRI-1 in Regulating Soybean Nodule Formation Under Cold Stress
Authors: Zhang Hongcai , He Lin , Li Huiyun , Tao Nengfu , Chang Tianda , Wang Dongmei , Lu Yichu , Li Zhenying , Mai Chunhai , Zhao Xiaorui , Niu Bingjie , Ma Junkui , Wang Lixiang
Published: January 2025

Symbiotic nitrogen fixation, recognized as the most efficient nitrogen assimilation system in ecosystems, is essential for soybean growth, as nodulation provides critical nitrogen to host cells. Soybeans thrive in warm and moist environments. However, they are highly susceptible to low temperatures, which impede the formation and development of root nodules. The genetic basis and molecular mechanism underlying the inhibition of nodulation induced by low temperatures remain unclear. In this study, we conducted a comparative transcriptomic analysis of soybean roots inoculated with rhizobium at 1 DPI (Day Post Inoculation) under normal or cold treatments. We identified 39 up-regulated and 35 down-regulated genes associated with nodulation and nitrogen fixation. Notably, cold-responsive genes including three FRI (Frigida) family genes were identified among differentially expressed genes (DEGs). Further expression pattern analysis of GmFRI-1 demonstrated it being significantly responsive to rhizobium inoculation and its highest expression in nodules. Further investigation revealed that overexpression of GmFRI-1 led to an increase in the nodule number, while RNA interference (RNAi)-mediated gene editing of GmFRI-1 suppressed nodule formation. Additionally, GmFRI-1 overexpression may regulate soybean nodulation by modulating the expression of GmNIN (NODULE INCEPTION), GmNSP1 (nodulation signaling pathway 1), and GmHAP2-2 (histone- or haem-associated protein domain) in the nod factor signaling pathway. This study offers new insights into the genetic basis of nodulation regulation under cold stress in legumes and indicates that GmFRI-1 may serve as a key regulator of nodule formation under cold stress.
Rapid Determination of Colistin Susceptibility by Flow Cytometry Directly from Positive Urine Samples—Preliminary Results
Authors: Fonseca-Silva Daniela , Gomes Rosário , Martins-Oliveira Inês , Silva-Dias Ana , Ramos Maria Helena , Pina-Vaz Cidália
Published: January 2025

Urinary tract infections caused by Gram-negative bacteria (GNB) are among the most common infections and a significant cause of sepsis. The increasing prevalence of multidrug-resistant (MDR) bacteria poses challenges to empirical treatment. Colistin may be used a last-resort antibiotic for treating MDR infections, but this requires the rapid determination of susceptibility to colistin. Traditional susceptibility testing methods can take up to 48 h, and there are specific challenges in determining colistin susceptibility. This study evaluates a novel, rapid method for determining colistin susceptibility directly from positive urine samples using the FASTcolistin MIC kit from FASTinov®. A total of 100 urine samples positive for Gram-negative bacilli when screened by the UF-1000i system were included in this study. After a simple sample prep, the same bacterial suspension was used for identification on MALDI-TOF and inoculated in the FASTcolistin MIC panel for our AST; after incubation at 37 °C for 1 h, it was analyzed via flow cytometry using a CytoFLEX cytometer (Beckman Coulter, Brea, CA, USA). The categorical susceptibility to colistin according to EUCAST or CLSI standards as well as the MIC values were given by bioFAST software (bioFAST 2.0). The essential agreement (EA) and bias were calculated. Different species of Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter spp. were correctly identified by MALDI-TOF directly from the FASTcolistin MIC sample prep. The essential agreement between the two methods was 99%, with a bias of −17%. Both identification and susceptibility were obtained in less than 2 h. This study presents a rapid and accurate method for determining colistin MIC directly from urine samples. The shortness of time required to produce a result, 2 h versus 48 h with the conventional methods, will significantly impact treatment decisions, especially in urinary tract infections difficult to treat.
Anomalies Classification in Fan Systems Using Dual-Branch Neural Networks with Continuous Wavelet Transform Layers: An Experimental Study
Authors: Pałczyński Cezary , Olejnik Paweł
Published: January 2025

In this study, anomalies in a fan system were classified using a real measurement setup to simulate mechanical anomalies such as blade detachment or debris accumulation. Data were collected under normal operating conditions and with an added unbalancing mass. Additionally, sensor anomalies were introduced by manipulating accelerometer readings and examining three types: spike, stuck, and dropout. To classify the anomalies, four neural network models—variations in Long Short-Term Memory (LSTM) and Convolutional Neural Network (CNN) were tested. These models incorporated a Continuous Wavelet Transform (CWT) layer. A novel approach for implementing the CWT layer in both LSTM and CNN architectures was proposed, along with a dual-branch input structure featuring two CWT layers using different mother wavelets. The dual-branch configuration with different mother wavelets yielded better accuracy for the simpler LSTM network. Accuracy comparisons were conducted for the 10 best-performing models based on validation set predictions, revealing improved classification performance. The study concluded with a summary of prediction accuracy for both the validation and test sets of data, along with the calculation of average accuracy, demonstrating the effectiveness of the proposed dual-branch neural network structure in classifying anomalies in fan systems.
Peripheral Defocus in Orthokeratology Myopia Correction: Systematic Review and Meta-Analysis
Authors: Queirós António , Pinheiro Inês , Fernandes Paulo
Published: January 2025

Background: This study aimed to assess the effect of peripheral defocus with orthokeratology lenses (PDOK) on myopia control in children and adolescents through a systematic review and meta-analysis. Methods: A comprehensive search was conducted in the PubMed and Web of Science databases to identify randomized controlled trials (RCTs) and cohort studies on PDOK, using the keywords “peripheral refraction” and “orthokeratology”. Studies were included if they reported spherical equivalent (M) peripheral refraction at 25° and/or 30° with accompanying statistical data along the horizontal meridian before and after orthokeratology treatment. From the initial 133 studies, those excluded included nine non-English publications, 18 reviews, five meta-analyses, four systematic reviews, and 88 studies not meeting the inclusion criteria. Results: Nine studies (three RCTs and six cohort studies) were included, involving 259 participants aged six to 30 years with a baseline refractive error of M = −2.44 ± 0.27 D, and treatment duration ranging from 14 days to 12 months. All the studies showed an increase in myopic defocus at 30° nasal (−2.55 ± 1.10 D) and temporal (−2.79 ± 0.75 D) eccentricities, averaging −2.67 ± 0.95 D across both. The overall induced myopic defocus was M = −2.56 D (95% CI: −2.21 to −2.91, Z = 14.33, p < 0.001), according to forest plot analysis. Studies with treatment durations up to one year showed a higher myopic blur (M = −2.69 D, 95% CI: −2.48 to −2.89, Z = 25.93, p < 0.001) compared to shorter treatments of less than three months (M = −2.39 D, 95% CI: −1.76 to −3.02, Z = 7.41, p < 0.001). Conclusions: Orthokeratology lenses effectively induce myopic defocus at 30° eccentricity over both short- and long-term treatments in children and adolescents, suggesting potential benefits for myopia control in these age groups.
Three-Dimensional Echocardiographic Assessment of Right Ventricular Global Myocardial Work and Ventricular–Pulmonary Coupling in ATTR Cardiac Amyloidosis
Authors: Venkateshvaran Ashwin , Edbom Fredrik , Arvidsson Sandra , Kovacs Attila , Lindqvist Per
Published: January 2025

Background: Right ventricular (RV) function is inadequately investigated and routinely overlooked in transthyretin amyloid cardiomyopathy (ATTR-CM). Novel imaging distinguishers between intrinsic RV myocardial disease in ATTR-CM and primary RV overload disorder phenotypes may enhance mechanistic and pathophysiological understanding of RV dysfunction. We aimed to investigate RV performance in ATTR-CM employing comprehensive 2D and 3D echocardiography, and to compare these indices with primary RV afterload disease. Methods: We investigated conventional and novel indices of RV contractile function, myocardial work and ventricular–vascular coupling in 21 well-characterized ATTR-CM patients, 10 PAH patients and 12 healthy controls. RV long axis function and pulmonary artery (PA) systolic pressure were evaluated using 2D Doppler echocardiography. RV ejection fraction (RVEF), volumes, global longitudinal strain (GLS) and novel myocardial work indices were analyzed by 3D echocardiography. RV elastance (Ees), afterload (Ea) and RV-PA coupling (Ees/Ea) were estimated using the single-beat volume method. Results: ATTR-CM showed lower RVEF, GLS and Ees, and a higher RV global myocardial work index (GWI), constructive work (GCW), Ea and reduced RV-PA coupling compared with controls. RV EF, stroke volume, GLS and circumferential strain did not differ between ATTR-CM and PAH. However, GWI, GCW, Ees and Ea were lower in ATTR-CM. RV–pulmonary coupling displayed strong association with RV 3D strain (r = 0.84, p < 0.001), whereas RV Ees (contractility) was related to RV GWI (r = 0.54, p < 0.001). Conclusions: ATTR-CM displayed lower RV performance, higher GMW and reduced RV-PA coupling. Myocardial work indices Ees and Ea are novel distinguishers of RV dysfunction phenotypes. The clinical and prognostic value of these novel variables warrant further investigation.
Pain, Function, and Elastosonographic Assessment After Shockwave Therapy in Non-Calcific Supraspinatus Tendinopathy: A Retrospective Observational Study
Authors: Santilli Gabriele , Ciccarelli Antonello , Martino Milvia , Pacini Patrizia , Agostini Francesco , Bernetti Andrea , Giuliani Luca , Del Gaudio Giovanni , Mangone Massimiliano , Colonna Vincenzo , Vetrano Mario , Vulpiani Maria Chiara , Stella Giulia , Taurone Samanta , Vigevano Federico , Cantisani Vito , Paoloni Marco , Fiore Pietro , Gimigliano Francesca
Published: January 2025

Background: Non-calcific supraspinatus tendinopathy (SNCCT) is a frequent cause of shoulder pain, often associated with functional impairment and reduced quality of life. Recent advancements in diagnostic imaging, including shear wave elastography (SWE), provide quantitative data on tendon stiffness and thickness, facilitating more precise evaluations. Extracorporeal shockwave therapy (ESWT) has emerged as a minimally invasive and effective treatment for SNCCT, but its effects on tendon properties measured through SWE require further investigation. Objective: This retrospective observational study aimed to evaluate the impact of ESWT on supraspinatus tendon characteristics in patients with SNCCT by assessing tendon thickness, SWE velocity, and clinical outcomes. Methods: This observational study enrolled 39 patients with SNCCT, aged 30–75 years, who received three ESWT sessions over 3 weeks. The intervention was delivered using a Modulith SLK system at an energy level of 0.20 mJ/mm2 with 2400 pulses per session. SWE and conventional ultrasound were used to measure tendon thickness and SWEv at baseline (T0) and 6 months post-treatment (T1). Clinical outcomes were assessed using the Visual Analog Scale (VAS), Constant and Murley Score (CMS), and modified Roles and Maudsley scale. Data were analyzed using paired t-tests and correlation analyses. Results: At baseline, affected tendons exhibited increased thickness (7.5 ± 0.9 mm) and reduced SWEv (3.1 ± 0.7 m/s) compared to healthy tendons (4.5 ± 0.7 mm and 6.9 ± 1 m/s, respectively; p < 0.05). Six months after ESWT, tendon thickness decreased significantly (6.2 ± 0.9 mm, p < 0.05), and SWEv increased (5.7 ± 1.8 m/s, p < 0.05), indicating improved elasticity. Clinical outcomes improved significantly, with the VAS scores decreasing from 6.5 ± 1.4 to 3.2 ± 2.1, the CMS score rising from 59.1 ± 17.3 to 78.2 ± 17.7, and the modified Roles and Maudsley scale improving from 2.3 ± 0.6 to 1.5 ± 0.8 (p < 0.05 for all). SWEv positively correlated with the CMS (r = 0.4) and negatively with the VAS and the modified Roles and Maudsley scale (r = −0.6 and r = −0.5, respectively). Conclusions: ESWT significantly reduces tendon thickness and enhances elasticity, correlating with improvements in pain and functional scores. SWE proved to be a reliable method for monitoring structural and clinical changes in SNCCT. Further research, including randomized controlled trials, is recommended to confirm these findings and explore longer-term outcomes.
Fungal Biocontrol Agents in the Management of Postharvest Losses of Fresh Produce—A Comprehensive Review
Authors: Ramudingana Phathutshedzo , Makhado Ndivhuho , Kamutando Casper Nyaradzai , Thantsha Mapitsi Silvester , Mamphogoro Tshifhiwa Paris
Published: January 2025

Postharvest decay of vegetables and fruits presents a significant threat confronting sustainable food production worldwide, and in the recent times, applying synthetic fungicides has become the most popular technique of managing postharvest losses. However, there are concerns and reported proofs of hazardous impacts on consumers’ health and the environment, traceable to the application of chemical treatments as preservatives on fresh produce. Physical methods, on the other hand, cause damage to fresh produce, exposing it to even more infections. Therefore, healthier and more environmentally friendly alternatives to existing methods for managing postharvest decays of fresh produce should be advocated. There is increasing consensus that utilization of biological control agents (BCAs), mainly fungi, represents a more sustainable and effective strategy for controlling postharvest losses compared to physical and chemical treatments. Secretion of antifungal compounds, parasitism, as well as competition for nutrients and space are the most common antagonistic mechanisms employed by these BCAs. This article provides an overview of (i) the methods currently used for management of postharvest diseases of fresh produce, highlighting their limitations, and (ii) the use of biocontrol agents as an alternative strategy for control of such diseases, with emphasis on fungal antagonists, their mode of action, and, more importantly, their advantages when compared to other methods commonly used. We therefore hypothesize that the use of fungal antagonists for prevention of postharvest loss of fresh produce is more effective compared to physical and chemical methods. Finally, particular attention is given to the gaps observed in establishing beneficial microbes as BCAs and factors that hamper their development, particularly in terms of shelf life, efficacy, commercialization, and legislation procedures.
Molecular Insights into the Role of the MET30 Protein and Its WD40 Domain in Colletotrichum gloeosporioides Growth and Virulence
Authors: Wu Fei , Sun Qianlong , Huang Longhui , Liu Sizhen , Chen Yue , Zhang Xin , Li Chenggang , Guo Sheng , Tan Xinqiu
Published: January 2025

Colletotrichum gloeosporioides is a major phytopathogen responsible for anthracnose in Capsicum annuum (pepper) which leads to significant yield losses. At present, the molecular mechanism of C. gloeosporioides pathogenesis is not very clear. In this study, we focused on the MET30 protein and its key WD40 domain, with an emphasis on its role in the biological functions of C. gloeosporioides. Bioinformatics analysis revealed that the MET30 protein contains a conserved F-box domain and multiple WD40 repeats, which interact with other proteins to participate in various cellular processes, including nutrient acquisition, stress responses, and pathogenicity. Gene knockout and complementation experiments demonstrated that deleting the MET30 protein or its WD40 domain significantly reduced the rates of spore production and hyphal growth while increasing tolerance to environmental stresses such as high salinity and oxidative stress. Furthermore, pathogenicity assays revealed that the WD40 domain of the MET30 protein is crucial for regulating fungal pathogenicity, as mutants lacking WD40 domains presented increased virulence on pepper leaves. These findings suggest that the WD40 domain, in synergy with the MET30 protein, regulates the pathogenicity and stress response of C. gloeosporioides, provides new insights into the molecular mechanisms of anthracnose, and offers potential strategies for effective disease control.
How Does Cross-City Patient Mobility Impact the Spatial Equity of Healthcare in China?
Authors: Xiang Bowen , Wei Wei , Guo Fang , Hong Mengyao
Published: January 2025

The phenomenon of patient mobility is becoming increasingly frequent, altering the actual service ranges of hospitals across various cities. However, its impact on the spatial equity of healthcare services at the national scale has yet to be fully explored. This paper aims to reveal the impact of intercity patient mobility on healthcare equity in China. Using one million patient mobility records from online healthcare platforms, we construct the 2023 Cross-City Patient Mobility Network in China and identify the patterns of cross-city patient mobility. Furthermore, we employ the Dagum Gini coefficient to measure the spatial disparities in per capita healthcare services before and after patient mobility. The results show that: (1) cross-city patient mobility exhibits administrative boundary effects and reflects the administrative hierarchy system, yet megacities extend their healthcare service ranges beyond provincial and urban agglomeration boundaries; (2) patient mobility enhances the equity of per capita healthcare services at both intra-provincial and inter-provincial levels, with inter-provincial disparities contributing significantly more than intra-provincial disparities—a trend further reinforced by patient mobility. This study not only provides a methodological framework for understanding the impact of patient mobility on the healthcare system but also offers empirical support for public health policymaking.