bluuebunny/crossref_metadata_2025 huggingface dataset:
Supramolecular Fractal Growth of Self-Assembled Fibrillar Networks
Authors: Nasr Pedram , Leung Hannah , Auzanneau France-Isabelle , Rogers Michael A.
Published: April 2021

Complex morphologies, as is the case in self-assembled fibrillar networks (SAFiNs) of 1,3:2,4-Dibenzylidene sorbitol (DBS), are often characterized by their Fractal dimension and not Euclidean. Self-similarity presents for DBS-polyethylene glycol (PEG) SAFiNs in the Cayley Tree branching pattern, similar box-counting fractal dimensions across length scales, and fractals derived from the Avrami model. Irrespective of the crystallization temperature, fractal values corresponded to limited diffusion aggregation and not ballistic particle–cluster aggregation. Additionally, the fractal dimension of the SAFiN was affected more by changes in solvent viscosity (e.g., PEG200 compared to PEG600) than crystallization temperature. Most surprising was the evidence of Cayley branching not only for the radial fibers within the spherulitic but also on the fiber surfaces.
Sex-Biased Gene Expression and Evolution in the Cerebrum and Syrinx of Chinese Hwamei (Garrulax canorus)
Authors: Jiang Hua , Lin Jian-Qing , Sun Li , Xu Yan-Chun , Fang Sheng-Guo
Published: April 2021

It is common that males and females display sexual dimorphisms, which usually result from sex-biased gene expression. Chinese hwamei (Garrulax canorus) is a good model for studying sex-biased gene expression because the song between the sexes is quite different. In this study, we analyze cerebrum and syrinx sex-biased gene expression and evolution using the de novo assembled Chinese hwamei transcriptome. In both the cerebrum and syrinx, our study revealed that most female-biased genes were actively expressed in females only, while most male-biased genes were actively expressed in both sexes. In addition, both male- and female-biased genes were enriched on the putative Z chromosome, suggesting the existence of sexually antagonistic genes and the insufficient dosage compensation of the Z-linked genes. We also identified a 9 Mb sex linkage region on the putative 4A chromosome which enriched more than 20% of female-biased genes. Resultantly, male-biased genes in both tissues had significantly higher Ka/Ks and effective number of codons (ENCs) than unbiased genes, and this suggested that male-biased genes which exhibit accelerated divergence may have resulted from positive selection. Taken together, our results initially revealed the reasons for the differences in singing behavior between males and females of Chinese hwamei.
Integrating Towed Underwater Video and Multibeam Acoustics for Marine Benthic Habitat Mapping and Fish Population Estimation
Authors: Ilich Alexander R. , Brizzolara Jennifer L. , Grasty Sarah E. , Gray John W. , Hommeyer Matthew , Lembke Chad , Locker Stanley D. , Silverman Alex , Switzer Theodore S. , Vivlamore Abigail , Murawski Steven A.
Published: April 2021

The west Florida shelf (WFS; Gulf of Mexico, USA) is an important area for commercial and recreational fishing, yet much of it remains unmapped and unexplored, hindering effective monitoring of fish stocks. The goals of this study were to map the habitat at an intensively fished area on the WFS known as “The Elbow”, assess the differences in fish communities among different habitat types, and estimate the abundance of each fish taxa within the study area. High-resolution multibeam bathymetric and backscatter data were combined with high-definition (HD) video data collected from a near-bottom towed vehicle to characterize benthic habitat as well as identify and enumerate fishes. Two semi-automated statistical classifiers were implemented for obtaining substrate maps. The supervised classification (random forest) performed significantly better (p = 0.001; α = 0.05) than the unsupervised classification (k-means clustering). Additionally, we found it was important to include predictors at a range of spatial scales. Significant differences were found in the fish community composition among the different habitat types, with both substrate and vertical relief found to be important with rock substrate and higher relief areas generally associated with greater fish density. Our results are consistent with the idea that offshore hard-bottom habitats, particularly those of higher vertical relief, serve as “essential fish habitat”, as these rocky habitats account for just 4% of the study area but 65% of the estimated total fish abundance. However, sand contributes 35% to total fish abundance despite comparably low densities due to its large area, indicating the importance of including these habitats in estimates of abundance as well. This work demonstrates the utility of combining towed underwater video sampling and multibeam echosounder maps for habitat mapping and estimation of fish abundance.
Effects of Recreational Small-Sided Soccer Games on Bone Mineral Density in Untrained Adults: A Systematic Review and Meta-Analysis
Authors: Clemente Filipe Manuel , Ramirez-Campillo Rodrigo , Sarmento Hugo , Castillo Daniel , Raya-González Javier , Rosemann Thomas , Knechtle Beat
Published: April 2021

This systematic review with meta-analysis was conducted to assess the effects of small-sided games (SSG)-based training programs on bone mineral density (BMD) in untrained adults. The data sources utilized were Cochrane, Embase, Medline (PubMed), Scopus, SPORTDiscus, and Web of Science. The study eligibility criteria were: (i) untrained adults (>18 years old) of any sex, with or without a noncommunicable disease; (ii) SSG-based programs with a minimum duration of four weeks and no restrictions regarding frequency (number of sessions per week); (iii) passive or active control groups; (iv) pre-post values of BMD; (v) only randomized controlled trials; and (vi) only original and full-text studies written in English. The database search initially yielded 374 titles. From those, nine articles were eligible for the systematic review and meta-analysis. The age of included population varied from a minimum of 20 and a maximum of 71 years old. Non-significant differences between SSG and passive and active control groups on total BMD (ES = 0.14; p = 0.405 and ES = 0.28; p = 0.05, respectively). Meanwhile, significant differences in favor of SSGs vs. passive and control groups were detected, evidencing an improvement of BMD in lower limbs of the adult population for both sexes (ES = 0.26; p = 0.05 and ES = 0.28; p = 0.156, respectively). As conclusions, SSGs can be used as a non-pharmacological alternative to increase the BMD in the lower limbs despite having no significant impact on total body BMD. Careful generalization should be done of the level of heterogeneity.
Is Misoprostol Vaginal Insert Safe for the Induction of Labor in High-Risk Pregnancy Obese Women?
Authors: Varlas Valentin Nicolae , Bostan Georgiana , Nasui Bogdana Adriana , Bacalbasa Nicolae , Pop Anca Lucia
Published: April 2021

Induction of labor (IOL) is an event that occurs in up to 25% of pregnancies. In Europe, the misoprostol vaginal insert (MVI—Misodel®) was approved for labor induction in 2013. Studies on the outcomes and safety of IOL in obese pregnant women are scarce; no data are available on MVI IOL in high-risk pregnancy obese women (HRPO—late-term, hypertension, diabetes). As the obesity rates are growing steadily in pregnant women, we aimed to evaluate the failure rate for induction and the safety of a 200 μg MVI in obese (body mass index (BMI) >30 kg/m2) HRPO compared to that for obese non-high-risk pregnancies (non-HRPO). For this purpose, we conducted a cross-sectional study in “Filantropia” Clinical Hospital, Bucharest, Romania, from June 2017—the date of the initiation of the MVI IOL protocol in our clinic—to September 2019. The primary outcomes were the failure rate, measured by cesarean section (CS) ratio, and secondarily, the safety profile of MVI, analyzed by one-way ANOVA. Out of a total of 11,096 registered live births, IOL was performed on 206 obese patients. Of these, 74 obese pregnant women had their labor induced with MVI (HRPO, n = 57, and non-HRPO, n = 17). The average maternal age was 29.9 ± 4.8 years (19–44 years). Across the groups, the rate of CS was 29.8% (n = 17) in the HRPO group compared to 23.5% (n = 4) in the non-HRPO group (p = non significant). In the vaginally birth subgroups, the median time from drug administration to delivery was shorter in the HRPO group compared to the non-HRPO group (16.9 ± 6.0 h 95% confidence interval (CI) 15.0–18.8 vs. 19.4 ± 9.2 h 95% CI 13.8–25.0, p = 0.03). No significant differences were found regarding the maternal outcomes among the studied groups; in terms of perinatal outcomes of safety, 5.4% (n = 4) of the cases of vaginal delivery for HRPO were associated with neonatal intensive care unit (NICU) admissions. The MVI seems to be an efficient labor induction agent in high-risk pregnancy obese women with good maternal outcomes and low perinatologic complications.
Enabling Food Environment in Kindergartens and Schools in Iran for Promoting Healthy Diet: Is It on the Right Track?
Authors: Omidvar Nasrin , Babashahi Mina , Abdollahi Zahra , Al-Jawaldeh Ayoub
Published: April 2021

Enabling policies related to kindergarten and school food environments can be effective approaches in preventing childhood obesity. This study investigated policies and/or programs with direct or indirect effects on the food environment in kindergartens and schools in Iran. In this scoping review, we systematically searched PubMed, Scopus, and Web of science, as well as Iranian scientific search engines, including Scientific Information Database and Magiran from January 1990 to October 2020, to identify literature on policies/programs affecting the food environment in kindergartens and schools in Iran. A total of 30 studies and policy documents were included in this review relevant to eight policies/programs. These programs have helped to control food availability in schools, increase nutritional awareness, positively influence physical function and school performances, and reduce malnutrition in rural kindergartens. However, improving the food environment in schools and kindergartens requires proper revisions and local adaptation of many of these policies, strengthening of cross-sectoral collaborations, provision of necessary financial and human resources, and ensuring regular monitoring and evaluation. Reflecting on Iran’s health policies and interventions provides insight into the progress achieved and challenges faced. Lessons can benefit the country itself, as well as other countries with similar contexts.
Does Work after Retirement Matter? Sleep Features among Workers in the Brazilian Longitudinal Study of Adult Health
Authors: Lowden Arne , Silva-Costa Aline , Rotenberg Lucia , Aquino Estela M. L. , Fonseca Maria de Jesus M. , Griep Rosane H.
Published: April 2021

A growing number of people keep working after retirement, a phenomenon known as bridge employment. Sleep features, which are related to morbidity and mortality outcomes, are expected to be influenced by bridge employment or permanent retirement. The objective of this study was to analyze sleep duration and quality of bridge employees and permanent retirees compared to nonretired, i.e., active workers, from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Participants (second wave of ELSA-Brasil, 2012–2014) comprised permanently retired (n = 2348), career bridge workers (n = 694), bridge workers in a different place (n = 760), and active workers (n = 6271). The associations of all studied retirement schemes and self-reported sleep quality and duration were estimated through logistic and linear regression analysis. Workers from all studied retirement schemes showed better sleep patterns than active workers. In comparison to active workers, bridge workers who had changed workplace also showed a reduced chance of difficulty falling asleep and too-early awakenings, which were not found among career bridge workers. Bridge employment and permanent retirement were associated with a reduced chance of reporting sleep deficit. Bridge work at a different place rather than staying at the same workplace seems to be favorable for sleep. Further study is needed to explain mechanisms.
Effectiveness and Safety of a Shorter Treatment Regimen in a Setting with a High Burden of Multidrug-Resistant Tuberculosis
Authors: Trubnikov Aleksandr , Hovhannesyan Arax , Akopyan Kristina , Ciobanu Ana , Sadirova Dilbar , Kalandarova Lola , Parpieva Nargiza , Gadoev Jamshid
Published: April 2021

Treatment of drug-resistant tuberculosis is lengthy, insufficiently effective, and toxic. Since 2016, the World Health Organization has recommended shorter treatment regimens (STR). We assessed effectiveness and predictors of drug adverse events (DAE) among patients treated with STR. There were 95 consecutive rifampicin-resistant patients enrolled in STR in Tashkent between June 2018 and September 2019. Of these, 66.3% were successfully treated, 17.9% suffered failed treatment, 7.4% died, 5.3% were lost to follow-up and 3.2% were not evaluated. No recurrence was identified in 54 patients after 12 months of successful treatment completion. There were 47 reported DAE: the incidence rate was 6.15 DAE per 100 person-months-of-treatment. Any DAE was reported in 38 (40%) patients and grade 3/4 DAE were recorded in 21 (22.1%) patients. Median time to DAE was 101 (interquartile range 64–139) days. The most frequently encountered DAE were gastro-intestinal disorders, followed by hepatotoxicity and ototoxicity. The most commonly offending drug inducing DAE was protionamide. The dose was temporarily interrupted in 55.3% of DAE, reduced in 8.5% of DAE and permanently withdrawn in another 8.5% of DAE. HIV status was the only predictor associated with increased hazard of DAE. In Uzbekistan STR showed moderate effectiveness and safety, although treatment failure was high.
Athletes’ Self-Assessment of Urine Color Using Two Color Charts to Determine Urine Concentration
Authors: Wardenaar Floris C. , Thompsett Daniel , Vento Kaila A. , Pesek Kathryn , Bacalzo Dean
Published: April 2021

Our objective was to determine self-reported accuracy of an athletic population using two different urine color (Uc) charts (8-color vs. 7-color Uc chart). After approval by the Institutional Review Board, members of an athletic population (n = 189, 20 (19–22) year old student- or tactical athletes and coaches, with n = 99 males and n = 90 females) scored their Uc using two charts. To determine the diagnostic value of Uc, results were compared with urine concentration (osmolality and urine specific gravity, USG). Uc was scored slightly darker with the 8-color vs. 7-color Uc chart (2.2 ± 1.2 vs. 2.0 ± 1.2, respectively, p < 0.001), with a moderate correlation between charts (r = 0.76, 95% CI: 0.69–0.81). Bland-Altman analysis showed a weak reporting bias (r = 0.15, p = 0.04). The area under the curve for correct urine sample classification ranged between 0.74 and 0.86. Higher accuracy for both methods was found when Uc scores were compared to USG over osmolality, indicated by 4.8–14.8% range in difference between methods. The optimal Uc cut-off value to assess a low vs. a high urine concentration for both Uc charts varied in this study between 1 and ≤2 while accuracy for charts was similar up to 77% when compared to USG.
Muscle Damage and Performance after Single and Multiple Simulated Matches in University Elite Female Soccer Players
Authors: Chou Tai-Ying , Nosaka Kazunori , Chen Trevor C.
Published: April 2021

The present study aimed to compare changes in muscle damage and performance parameters after playing single versus multiple soccer matches to examine fixture congestion effects on performance. Twelve elite female university soccer players performed single, three and six consecutive 90-min bouts of the Loughborough Intermittent Shuttle Test (LIST) with ≥12-weeks between conditions in a pseudo-randomized order. Heart rate, blood lactate, rating of perceived exertion and covering distance in each LIST were examined. Changes in several types of muscle damage (e.g., maximal voluntary isometric torque of the knee extensors: MVC-KE) and performance measures (e.g., Yo-Yo Intermittent Recovery Test level 1: YYIR1) were taken before each LIST, 1 h, and 1–5 d after the last LIST. The total distance covered during the LIST was shorter (p < 0.05) in the 2nd–3rd, or 2nd–6th LISTs when compared with the 1st LIST. Changes (p < 0.05) in all measures were observed after the LIST, and the greatest changes were observed after the six than after the three LISTs followed by one LIST (e.g., largest changes in MVC-KE: −26 > −20 > −14%; YYIR1: −31 > −26 > −11%). Many of the variables did not recover to the baseline for 5 d after six LISTs. These suggest that fixture congestion induces greater muscle damage and performance decline than a single match.
Effects of an Alcohol-Related Harm Prevention Program among Out-of-School Female Adolescents
Authors: Park Hyojin , Kim Sungjae , Yang Jeongwoon
Published: April 2021

This study aimed to evaluate the effects of an alcohol-related harm prevention program on out-of-school adolescent girls. This was a quasi-experimental study employing a randomized controlled non-synchronized design. There were 23 and 22 participants in the experimental and control groups, respectively. The program comprised three sessions aiming to motivate voluntary changes and promote autonomous decision-making. The experimental group showed significantly higher alcohol-related knowledge and substantially lower alcohol outcome expectancy than the control group. No significant differences emerged from drinking refusal, self-efficacy, or alcohol abstinence intention. This program could improve alcohol-related knowledge and reduce out-of-school adolescent girls’ positive alcohol outcome expectancy.
Translating Co-Design from Face-to-Face to Online: An Australian Primary Producer Project Conducted during COVID-19
Authors: Kennedy Alison , Cosgrave Catherine , Macdonald Joanna , Gunn Kate , Dietrich Timo , Brumby Susan
Published: April 2021

Primary producers face considerable risks for poor mental health. While this population can be difficult to engage in programs to prevent poor mental health, approaches tailored to reflect the context of primary producers’ life and work have been successful. This paper reports on the co-design phase of a project designed to prevent poor mental health for primary producers—specifically, the advantages, challenges and considerations of translating face-to-face co-design methods to an online environment in response to COVID-19 restrictions. The co-design phase drew upon the existing seven-step co-design framework developed by Trischler and colleagues. Online methods were adopted for all steps of the process. This paper models how this co-design approach can work in an online, primary producer context and details key considerations for future initiatives of this type. The development of online co-design methods is an important additional research method for use not only during a pandemic but also when operating with limited resources or geographic constraints. Results demonstrate the following: (i) co-designing online is possible given adequate preparation, training and resource allocation; (ii) “hard to reach” populations can be engaged using online methods providing there is adequate early-stage relationship building; (iii) co-design quality need not be compromised and may be improved when translating to online; and (iv) saved costs and resources associated with online methods can be realigned towards intervention/service creation, promotion and user engagement. Suggestions for extending Trischler and colleagues’ model are incorporated.
Perception and Demands of Pregnant and Breastfeeding Women Regarding Their Role as Participants in Environmental Research Studies
Authors: Company-Morales Miguel , Zafra Aparici Eva , Casadó Lina , Alarcón Montenegro Cristina , Arrebola Juan Pedro
Published: April 2021

A significant proportion of scientific studies consider pregnant and breastfeeding women as vulnerable subjects. The objective of this study was to analyse the perception of pregnant and breastfeeding women regarding their participation in environmental research studies. Our work is a descriptive and interpretative observational study that has been developed under the qualitative research paradigm following a phenomenological and ethnographic perspective. The study involved 173 women selected intentionally in two Spanish autonomous communities. To obtain the primary data, we relied upon 111 interviews, four focused ethnographies and eight focus groups. The data encoding and analysis was carried out with the help of NVivo 12 software (QSR International, Boston, MA, USA). We evidenced the need of pregnant and breastfeeding women for more detailed and accurate information on the risk of environmental pollutant exposure during their crucial life stage. In addition, these women claimed for a more participatory role in research studies. Pregnant and breastfeeding women in Spain ask for greater interaction with researchers and propose a dialogical relationship between valid partners. We conclude that our pregnant and breastfeeding women claim more research focused on their collective, as well as clearer, more accessible and structured information on the risks of exposure to environmental contaminants. In addition, they do not want to simply be informants; rather, they ask to be active and empowered members by providing their opinions and arguments throughout the research process.
Emission Inventories and Particulate Matter Air Quality Modeling over the Pearl River Delta Region
Authors: Lopes Diogo , Ferreira Joana , Hoi Ka In , Yuen Ka-Veng , Mok Kai Meng , Miranda Ana I.
Published: April 2021

The Pearl River Delta (PRD) region is located on the southeast coast of mainland China and it is an important economic hub. The high levels of particulate matter (PM) in the atmosphere, however, and poor visibility have become a complex environmental problem for the region. Air quality modeling systems are useful to understand the temporal and spatial distribution of air pollution, making use of atmospheric emission data as inputs. Over the years, several atmospheric emission inventories have been developed for the Asia region. The main purpose of this work is to evaluate the performance of the air quality modeling system for simulating PM concentrations over the PRD using three atmospheric emission inventories (i.e., EDGAR, REAS and MIX) during a winter and a summer period. In general, there is a tendency to underestimate PM levels, but results based on the EDGAR emission inventory show slightly better accuracy. However, improvements in the spatial and temporal disaggregation of emissions are still needed to properly represent PRD air quality. This study’s comparison of the three emission inventories’ data, as well as their PM simulating outcomes, generates recommendations for future improvements to atmospheric emission inventories and our understanding of air pollution problems in the PRD region.
Analysis of OpenStreetMap Data Quality at Different Stages of a Participatory Mapping Process: Evidence from Slums in Africa and Asia
Authors: Yeboah Godwin , Porto de Albuquerque João , Troilo Rafael , Tregonning Grant , Perera Shanaka , Ahmed Syed A. K. Shifat , Ajisola Motunrayo , Alam Ornob , Aujla Navneet , Azam Syed Iqbal , Azeem Kehkashan , Bakibinga Pauline , Chen Yen-Fu , Choudhury Nazratun Nayeem , Diggle Peter J. , Fayehun Olufunke , Gill Paramjit , Griffiths Frances , Harris Bronwyn , Iqbal Romaina , Kabaria Caroline , Ziraba Abdhalah Kasiira , Khan Afreen Zaman , Kibe Peter , Kisia Lyagamula , Kyobutungi Catherine , Lilford Richard J. , Madan Jason J. , Mbaya Nelson , Mberu Blessing , Mohamed Shukri F. , Muir Helen , Nazish Ahsana , Njeri Anne , Odubanjo Oladoyin , Omigbodun Akinyinka , Osuh Mary E. , Owoaje Eme , Oyebode Oyinlola , Pitidis Vangelis , Rahman Omar , Rizvi Narjis , Sartori Jo , Smith Simon , Taiwo Olalekan John , Ulbrich Philipp , Uthman Olalekan A. , Watson Samuel I. , Wilson Ria , Yusuf Rita
Published: April 2021

This paper examines OpenStreetMap data quality at different stages of a participatory mapping process in seven slums in Africa and Asia. Data were drawn from an OpenStreetMap-based participatory mapping process developed as part of a research project focusing on understanding inequalities in healthcare access of slum residents in the Global South. Descriptive statistics and qualitative analysis were employed to examine the following research question: What is the spatial data quality of collaborative remote mapping achieved by volunteer mappers in morphologically complex urban areas? Findings show that the completeness achieved by remote mapping largely depends on the morphology and characteristics of slums such as building density and rooftop architecture, varying from 84% in the best case, to zero in the most difficult site. The major scientific contribution of this study is to provide evidence on the spatial data quality of remotely mapped data through volunteer mapping efforts in morphologically complex urban areas such as slums; the results could provide insights into how much fieldwork would be needed in what level of complexity and to what extent the involvement of local volunteers in these efforts is required.
The Role of Selected Wavelengths of Light in the Activity of Photosystem II in Gloeobacter violaceus
Authors: Kula-Maximenko Monika , Zieliński Kamil Jan , Ślesak Ireneusz
Published: April 2021

Gloeobacter violaceus is a cyanobacteria species with a lack of thylakoids, while photosynthetic antennas, i.e., phycobilisomes (PBSs), photosystem II (PSII), and I (PSI), are located in the cytoplasmic membrane. We verified the hypothesis that blue–red (BR) light supplemented with a far-red (FR), ultraviolet A (UVA), and green (G) light can affect the photosynthetic electron transport chain in PSII and explain the differences in the growth of the G. violaceus culture. The cyanobacteria were cultured under different light conditions. The largest increase in G. violaceus biomass was observed only under BR + FR and BR + G light. Moreover, the shape of the G. violaceus cells was modified by the spectrum with the addition of G light. Furthermore, it was found that both the spectral composition of light and age of the cyanobacterial culture affect the different content of phycobiliproteins in the photosynthetic antennas (PBS). Most likely, in cells grown under light conditions with the addition of FR and G light, the average antenna size increased due to the inactivation of some reaction centers in PSII. Moreover, the role of PSI and gloeorhodopsin as supplementary sources of metabolic energy in the G. violaceus growth is discussed.
How Can a Polymeric Formula Induce Remission in Crohn’s Disease Patients?
Authors: Boumessid Kawthar , Barreau Frederick , Mas Emmanuel
Published: April 2021

Crohn’s disease is an inflammatory bowel disease whose prevalence is increasing worldwide. Among medical strategies, dietary therapy with exclusive enteral nutrition is recommended as a first-line option, at least for children, because it induces clinical remission and mucosal healing. Modulen®, a polymeric TGF-β2 enriched formula, has good palatability and is widely used. For the first time in the literature, this review outlines and discusses the clinical outcomes obtained with this therapy, as well as the potential mechanisms of action of its compounds. It can be explained by its TGF-β2 content, but also by its protein and lipid composition. Further well-designed studies are required to improve our knowledge and to optimize therapeutic strategies.
Epigallocatechin Gallate for Management of Heavy Metal-Induced Oxidative Stress: Mechanisms of Action, Efficacy, and Concerns
Authors: Zwolak Iwona
Published: April 2021

In this review, we highlight the effects of epigallocatechin gallate (EGCG) against toxicities induced by heavy metals (HMs). This most active green tea polyphenol was demonstrated to reduce HM toxicity in such cells and tissues as testis, liver, kidney, and neural cells. Several protective mechanisms that seem to play a pivotal role in EGCG-induced effects, including reactive oxygen species scavenging, HM chelation, activation of nuclear factor erythroid 2-related factor 2 (Nrf2), anti-inflammatory effects, and protection of mitochondria, are described. However, some studies, especially in vitro experiments, reported potentiation of harmful HM actions in the presence of EGCG. The adverse impact of EGCG on HM toxicity may be explained by such events as autooxidation of EGCG, EGCG-mediated iron (Fe3+) reduction, depletion of intracellular glutathione (GSH) levels, and disruption of mitochondrial functions. Furthermore, challenges hampering the potential EGCG application related to its low bioavailability and proper dosing are also discussed. Overall, in this review, we point out insights into mechanisms that might account for both the beneficial and adverse effects of EGCG in HM poisoning, which may have a bearing on the design of new therapeutics for HM intoxication therapy.
Mutations Q93H and E97K in TPM2 Disrupt Ca-Dependent Regulation of Actin Filaments
Authors: Śliwinska Małgorzata , Robaszkiewicz Katarzyna , Wasąg Piotr , Moraczewska Joanna
Published: April 2021

Tropomyosin is a two-chain coiled coil protein, which together with the troponin complex controls interactions of actin with myosin in a Ca2+-dependent manner. In fast skeletal muscle, the contractile actin filaments are regulated by tropomyosin isoforms Tpm1.1 and Tpm2.2, which form homo- and heterodimers. Mutations in the TPM2 gene encoding isoform Tpm2.2 are linked to distal arthrogryposis and congenital myopathy—skeletal muscle diseases characterized by hyper- and hypocontractile phenotypes, respectively. In this work, in vitro functional assays were used to elucidate the molecular mechanisms of mutations Q93H and E97K in TPM2. Both mutations tended to decrease actin affinity of homo-and heterodimers in the absence and presence of troponin and Ca2+, although the effect of Q93H was stronger. Changes in susceptibility of tropomyosin to trypsin digestion suggested that the mutations diversified dynamics of tropomyosin homo- and heterodimers on the filament. The presence of Q93H in homo- and heterodimers strongly decreased activation of the actomyosin ATPase and reduced sensitivity of the thin filament to [Ca2+]. In contrast, the presence of E97K caused hyperactivation of the ATPase and increased sensitivity to [Ca2+]. In conclusion, the hypo- and hypercontractile phenotypes associated with mutations Q93H and E97K in Tpm2.2 are caused by defects in Ca2+-dependent regulation of actin–myosin interactions.
Prophylaxis and Treatment against Klebsiella pneumoniae: Current Insights on This Emerging Anti-Microbial Resistant Global Threat
Authors: Arato Vanessa , Raso Maria Michelina , Gasperini Gianmarco , Berlanda Scorza Francesco , Micoli Francesca
Published: April 2021

Klebsiella pneumoniae (Kp) is an opportunistic pathogen and the leading cause of healthcare-associated infections, mostly affecting subjects with compromised immune systems or suffering from concurrent bacterial infections. However, the dramatic increase in hypervirulent strains and the emergence of new multidrug-resistant clones resulted in Kp occurrence among previously healthy people and in increased morbidity and mortality, including neonatal sepsis and death across low- and middle-income countries. As a consequence, carbapenem-resistant and extended spectrum β-lactamase-producing Kp have been prioritized as a critical anti-microbial resistance threat by the World Health Organization and this has renewed the interest of the scientific community in developing a vaccine as well as treatments alternative to the now ineffective antibiotics. Capsule polysaccharide is the most important virulence factor of Kp and plays major roles in the pathogenesis but its high variability (more than 100 different types have been reported) makes the identification of a universal treatment or prevention strategy very challenging. However, less variable virulence factors such as the O-Antigen, outer membrane proteins as fimbriae and siderophores might also be key players in the fight against Kp infections. Here, we review elements of the current status of the epidemiology and the molecular pathogenesis of Kp and explore specific bacterial antigens as potential targets for both prophylactic and therapeutic solutions.
Influence of Glycerophosphate Salt Solubility on the Gelation Mechanism of Colloidal Chitosan Systems
Authors: Owczarz Piotr , Rył Anna , Sowiński Jarosław
Published: April 2021

Recently, thermosensitive chitosan systems have attracted the interest of many researchers due to their growing application potential. Nevertheless, the mechanism of the sol-gel phase transition is still being discussed, and the glycerophosphate salt role is ambiguous. The aim of the work is to analyze the possibility of the exclusive use of a non-sodium glycerophosphate salt and to determine its impact on the gelation conditions determined by rheological and turbidimetric measurements as well as the stability of the systems by measuring changes in the Zeta potential value. It was found that ensuring the same proportions of glycerophosphate ions differing in cation to amino groups present in chitosan chains, leads to obtaining systems significantly different in viscoelastic properties and phase transition conditions. It was clearly shown that the systems with the calcium glycerophosphate, the insoluble form of which may constitute additional aggregation nuclei, undergo the gelation the fastest. The use of magnesium glycerophosphate salt delays the gelation due to the heat-induced dissolution of the salt. Thus, it was unequivocally demonstrated that the formulation of the gelation mechanism of thermosensitive chitosan systems based solely on the concentration of glycerophosphate without discussing its type is incorrect.
Polyacylated Anthocyanins in Bluish-Purple Petals of Chinese Bellflower, Platycodon grandiflorum
Authors: Kondo Tadao , Hagihara Seiji , Takaya Yoshiaki , Yoshida Kumi
Published: April 2021

The bluish-purple petals of Chinese bellflower, Platycodon grandiflorum (kikyo in Japanese), contain platyconin (1) as the major anthocyanin. Platyconin (1) is a polyacylated anthocyanin with two caffeoyl residues at the 7-position, and its color is stable in a diluted, weakly acidic aqueous solutions. HPLC analysis of the fresh petal extract showed the presence of several minor pigments. Photo-diode array detection of minor pigments suggested that some of these were polyacylated anthocyanins. To establish the relationship between structure and stability of the acylated anthocyanins and to obtain information on their biosynthetic pathways, minor pigments were isolated from the petals, and their structures were determined by MS and NMR analyses. Four known (2–5) and three new anthocyanins (6–8) were identified, which contained a delphinidin chromophore, and four of these (5–8) were diacylated anthocyanins, in which the acyl-glucosyl-acyl-glucosyl chain was attached at the 7-O-position of the delphinidin chromophore. These diacylated anthocyanins exhibited a bluish-purple color at pH 6, which was stable for more than a week.
Elucidating the Multi-Targeted Role of Nutraceuticals: A Complementary Therapy to Starve Neurodegenerative Diseases
Authors: Behl Tapan , Kaur Gagandeep , Sehgal Aayush , Singh Sukhbir , Bhatia Saurabh , Al-Harrasi Ahmed , Zengin Gokhan , Bungau Simona Gabriela , Munteanu Mihai Alexandru , Brisc Mihaela Cristina , Andronie-Cioara Felicia Liana , Brisc Ciprian
Published: April 2021

The mechanisms underlying multifactorial diseases are always complex and challenging. Neurodegenerative disorders (NDs) are common around the globe, posing a critical healthcare issue and financial burden to the country. However, integrative evidence implies some common shared mechanisms and pathways in NDs, which include mitochondrial dysfunction, neuroinflammation, oxidative stress, intracellular calcium overload, protein aggregates, oxidative stress (OS), and neuronal destruction in specific regions of the brain, owing to multifaceted pathologies. The co-existence of these multiple pathways often limits the advantages of available therapies. The nutraceutical-based approach has opened the doors to target these common multifaceted pathways in a slow and more physiological manner to starve the NDs. Peer-reviewed articles were searched via MEDLINE and PubMed published to date for in-depth research and database collection. Considered to be complementary therapy with current clinical management and common drug therapy, the intake of nutraceuticals is considered safe to target multiple mechanisms of action in NDs. The current review summarizes the popular nutraceuticals showing different effects (anti-inflammatory, antioxidant, neuro-protectant, mitochondrial homeostasis, neurogenesis promotion, and autophagy regulation) on vital molecular mechanisms involved in NDs, which can be considered as complementary therapy to first-line treatment. Moreover, owing to its natural source, lower toxicity, therapeutic interventions, biocompatibility, potential nutritional effects, and presence of various anti-oxidative and neuroprotective constituents, the nutraceuticals serve as an attractive option to tackle NDs.
The Influence of UV Light on Photodegradation of Acetylsalicylic Acid
Authors: Daescu Monica , Iota Miruna , Serbschi Constantin , Ion Alina C. , Baibarac Mihaela
Published: April 2021

Photodegradation of the aqueous solutions of acetylsalicylic acid, in the absence (ASA) and the presence of excipients (ASE), is demonstrated by the photoluminescence (PL). A shift of the PL bands from 342 and 338 nm to 358 and 361–397 nm for ASA and ASE in solid state and as aqueous solutions was reported. By exposure of the solution of ASA 0.3 M to UV light, a decrease in the PL band intensity was highlighted. This behavior was revealed for ASA in the presence of phosphate buffer (PB) having the pH equal to 6.4, 7, and 8 or by the interaction with NaOH 0.3 M. A different behavior was reported in the case of ASE. In the presence of PB, an increase in the intensity of the PL band of ASE simultaneously with a change of the ratio between the intensities of the bands at 361–364 and 394–397 nm was highlighted. The differences between PL spectra of ASA and ASE have their origin in the presence of salicylic acid (SAL). The interaction of ASE with NaOH induces a shift of the PL band at 405–407 nm. Arguments for the reaction of ASA with NaOH are shown by Raman scattering and FTIR spectroscopy.
RDAMS: An Efficient Run-Time Approach for Memory Fault and Hardware Trojans Detection
Authors: Wang Jian , Li Ying
Published: April 2021

Ensuring the security of IoT devices and chips at runtime has become an urgent task as they have been widely used in human life. Embedded memories are vital components of SoC (System on Chip) in these devices. If they are attacked or incur faults at runtime, it will bring huge losses. In this paper, we propose a run-time detection architecture for memory security (RDAMS) to detect memory threats (fault and Hardware Trojans attack). The architecture consists of a Security Detection Core (SDC) that controls and enforces the detection procedure as a “security brain”, and a memory wrapper (MEM_wrapper) which interacts with memory to assist the detection. We also design a low latency response mechanism to solve the SoC performance degradation caused by run-time detection. A block-based multi-granularity detection approach is proposed to render the design flexible and reduce the cost in implementation using the FPGA’s dynamic partial reconfigurable (DPR) technology, which enables online detection mode reconfiguration according to the requirements. Experimental results show that RDAMS can correctly detect and identify 10 modeled memory faults and two types of Hardware Trojans (HTs) attacks without leading a great performance degradation to the system.
Understanding Why Post-Stroke Depression May Be the Norm Rather Than the Exception: The Anatomical and Neuroinflammatory Correlates of Post-Stroke Depression
Authors: Wijeratne Tissa , Sales Carmela
Published: April 2021

Ischemic Stroke precedes depression. Post-stroke depression (PSD) is a major driver for poor recovery, negative quality of life, poor rehabilitation outcomes and poor functional ability. In this systematic review, we analysed the inflammatory basis of post-stroke depression, which involves bioenergetic failure, deranged iron homeostasis (calcium influx, Na influx, potassium efflux etc), excitotoxicity, acidotoxicity, disruption of the blood brain barrier, cytokine-mediated cytotoxicity, reactive oxygen mediated toxicity, activation of cyclooxygenase pathway and generation of toxic products. This process subsequently results in cell death, maladapted, persistent neuro-inflammation and deranged neuronal networks in mood-related brain regions. Furthermore, an in-depth review likewise reveals that anatomic structures related to post-stroke depression may be localized to complex circuitries involving the cortical and subcortical regions.
Tumoral Phenocopies of Hypertrophic Cardiomyopathy: The Role of Cardiac Magnetic Resonance
Authors: Bombace Sara , My Ilaria , Francone Marco , Monti Lorenzo
Published: April 2021

Hypertrophic cardiomyopathy (HCM) is a genetic cardiac disease that presents with cardiac hypertrophy. HCM phenocopies are clinical conditions that are phenotypically undistinguishable from HCM, but with a different underlying etiology. Cardiac tumors are rare entities that can sometimes mimic HCM in their echocardiographic appearance, thus representing an example of HCM phenocopy. At present, only case reports of tumoral HCM phenocopies can be found in literature. In this systematic review, we analyzed all the published cases in which a cardiac tumor mimicked HCM to the point of misleading the diagnosis, providing a structured overview of the currently available evidence on this topic.
From the Reef to the Ocean: Revealing the Acoustic Range of the Biophony of a Coral Reef (Moorea Island, French Polynesia)
Authors: Raick Xavier , Di Iorio Lucia , Gervaise Cédric , Lossent Julie , Lecchini David , Parmentier Éric
Published: April 2021

The ability of different marine species to use acoustic cues to locate reefs is known, but the maximal propagation distance of coral reef sounds is still unknown. Using drifting antennas (made of a floater and an autonomous recorder connected to a hydrophone), six transects were realized from the reef crest up to 10 km in the open ocean on Moorea island (French Polynesia). Benthic invertebrates were the major contributors to the ambient noise, producing acoustic mass phenomena (3.5–5.5 kHz) that could propagate at more than 90 km under flat/calm sea conditions and more than 50 km with an average wind regime of 6 knots. However, fish choruses, with frequencies mainly between 200 and 500 Hz would not propagate at distances greater than 2 km. These distances decreased with increasing wind or ship traffic. Using audiograms of different taxa, we estimated that fish post-larvae and invertebrates likely hear the reef at distances up to 0.5 km and some cetaceans would be able to detect reefs up to more than 17 km. These results are an empirically based validation from an example reef and are essential to understanding the effect of soundscape degradation on different zoological groups.
Analysis of Dangerous Sea States in the Northwestern Mediterranean Area
Authors: Innocenti Alessio , Onorato Miguel , Brandini Carlo
Published: April 2021

Extreme sea waves, although rare, can be notably dangerous when associated with energetic sea states and can generate risks for the navigation. In the last few years, they have been the object of extensive research from the scientific community that helped with understanding the main physical aspects; however, the estimate of extreme waves probability in operational forecasts is still debated. In this study, we analyzed a number of sea-states that occurred in a precise area of the Mediterranean sea, near the location of a reported accident, with the objective of relating the probability of extreme events with different sea state conditions. For this purpose, we performed phase-resolving simulations of wave spectra obtained from a WaveWatch III hindcast, using a Higher Order Spectral Method. We produced statistics of the sea-surface elevation field, calculating crest distributions and the probability of extreme events from the analysis of a long time-series of the surface elevation. We found a good matching between the distributions of the numerically simulated field and theory, namely Tayfun second- and third- order ones, in contrast with a significant underestimate given by the Rayleigh distribution. We then related spectral quantities like angular spreading and wave steepness to the probability of occurrence of extreme events finding an enhanced probability for high mean steepness seas and narrow spectra, in accordance with literature results, finding also that the case study of the reported accident was not amongst the most dangerous. Finally, we related the skewness and kurtosis of the surface elevation to the wave steepness to explain the discrepancy between theoretical and numerical distributions.
Genome-Wide Identification and Analysis of Nilaparvata lugens microRNAs during Challenge with the Entomopathogenic Fungus Metarhizium anisopliae
Authors: Xie Jiaqin , Peng Yifan , Xia Yuxian
Published: April 2021

The resistance of the notorious rice pest Nilaparvata lugens to many insecticides has caused significant concerns. Our previous study demonstrated that the fungus Metarhizium anisopliae CQMa421 shows great potential for the control of this pest, but the interactions between them are still unclear. Thus, we further investigated fungal infection-related microRNAs (miRNAs) in N. lugens during M. anisopliae CQMa421 challenge using Illumina sequencing. In this study, we constructed twenty-four small RNA libraries over different time courses (i.e., 4 h, 8 h, 16 h, and 24 h). A total of 478.62 M clean reads were collected, with each sample producing more than 13.37 M reads, after the removal of low-quality reads. We identified 2324 miRNAs and their 11,076 target genes within the twenty-four libraries by bioinformatics analysis. Differentially expressed miRNAs (DEmiRNAs), including 58 (32 upregulated vs. 26 downregulated), 62 (30 upregulated vs. 32 downregulated), 126 (71 upregulated vs. 55 downregulated), and 109 (40 upregulated vs. 69 downregulated) DEmiRNAs were identified at 4 h, 8 h, 16 h, and 24 h post-infection, respectively. We further conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to predict the functions of all target genes of DEmiRNAs. These DEmiRNAs targets identified during 24 h of infection were primarily involved in energy metabolism, lysine degradation, the FoxO signaling pathway, ubiquitin-mediated proteolysis, the mRNA surveillance pathway, and the MAPK signaling pathway. Taken together, our results provide essential information for further study of the interactions between the entomopathogenic fungus M. anisopliae and N. lugens at the posttranscriptional level.