bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Optimal Parameters for a Small-Scale Compressed-Air Energy Storage System Using Real Coded Genetic Algorithm
Authors: Guewouo Thomas , Luo Lingai , Tarlet Dominique , Tazerout Mohand
Published: January 2019

Compressed-Air energy storage (CAES) is a well-established technology for storing the excess of electricity produced by and available on the power grid during off-peak hours. A drawback of the existing technique relates to the need to burn some fuel in the discharge phase. Sometimes, the design parameters used for the simulation of the new technique are randomly chosen, making their actual construction difficult or impossible. That is why, in this paper, a small-scale CAES without fossil fuel is proposed, analyzed, and optimized to identify the set of its optimal design parameters maximizing its performances. The performance of the system is investigated by global exergy efficiency obtained from energy and exergy analyses methods and used as an objective function for the optimization process. A modified Real Coded Genetic Algorithm (RCGA) is used to maximize the global exergy efficiency depending on thirteen design parameters. The results of the optimization indicate that corresponding to the optimum operating point, the consumed compressor electric energy is 103.83 kWh and the electric energy output is 25.82 kWh for the system charging and discharging times of about 8.7 and 2 h, respectively. To this same optimum operating point, a global exergy efficiency of 24.87% is achieved. Moreover, if the heat removed during the compression phase is accounted for in system efficiency evaluation based on the First Law of Thermodynamics, an optimal round-trip efficiency of 79.07% can be achieved. By systematically analyzing the variation of all design parameters during evolution in the optimization process, we conclude that the pneumatic motor mass flow rate can be set as constant and equal to its smallest possible value. Finally, a sensitivity analysis performed with the remaining parameters for the change in the global exergy efficiency shows the impact of each of these parameters.
Subverting the Nation-State Through Post-Partition Nostalgia: Joginder Paul’s Sleepwalkers
Authors: Ghosh Amrita
Published: January 2019

With the advent of the Progressive Writers Movement, Urdu Literature was marked with a heightened form of social realism during the Partition of British India in 1947. Joginder Paul, once a part of this movement, breaks away from this realist tradition in his Urdu novella, Khwabrau (Sleepwalkers), published in 1990. Sleepwalkers shifts the dominant realist strain in the form and content of Urdu fiction to open a liminal “third space” that subverts the notion of hegemonic reality. Sleepwalkers is based on a time, many years after the Partition in the city of Karachi, and focuses on the “mohajirs” from Lucknow who construct a mnemonic existential space by constructing a simulacrum of pre-Partition Lucknow (now in India). This paper examines the reconceptualization of spaces through the realm of political nostalgia and the figure of the refugee subject “performing” this nostalgia. This nostalgic reconstruction of space, thus, becomes a “heterotopia” in Foucauldian terms, one that causes a rupture in the unities of time and space and the idea of nation-hood. The refugee subjects’ subversion of the linearity of time opens a different time in the narration of a nation that necessitates that the wholeness of the “imagined” physical space of a nation be questioned.
Like Father, Like Son. Physical Activity, Dietary Intake, and Media Consumption in Pre-School-Aged Children
Authors: Frate Nadja , Jenull Brigitte , Birnbacher Robert
Published: January 2019

An imbalance between energy input and energy needs contributes to the growing incidence of overweight children. Pre-schoolers normally like to move, but even at this young age, they are already affected by a lack of physical activity and a high amount of screen time. Media consumption contributes to unhealthy diets and extends the length of time spent sitting. Longer periods of sitting are, independent of the level of activity, seen as a risk factor for the development of obesity. In the present study, 160 pre-schoolers and their parents (128 mothers, 121 fathers) were examined. The results show deviations from actual recommendations regarding physical activity, time spent sitting, dietary intake, and media consumption. Increased screen time was associated with a higher weight status among pre-school-aged children. To provide a healthy upbringing, prevention and intervention measures should be implemented on a behavioral and relational level.
An Explanatory Model of Potential Changes in Burnout Diagnosis According to Personality Factors in Oncology Nurses
Authors: De la Fuente-Solana Emilia I. , Cañadas Gustavo R. , Ramirez-Baena Lucia , Gómez-Urquiza Jose L. , Ariza Tania , Cañadas-De la Fuente Guillermo A.
Published: January 2019

Burnout in a hospital oncology service takes place when there is a high level of interaction between nurses and patients. The aim of the present study is to identify models that will enable us to accurately classify a person at a given level within each of the three dimensions of burnout, according to the values presented for personality related explanatory variables, for a sample of 96 oncology nurses working in the regional health service of Andalusia (Spain). A quantitative, crosscutting, multicentre, descriptive study was designed, and for this purpose data on sociodemographic and personality variables and on the three dimensions of burnout were compiled. Three categorical-response logit ordinal models were used and the prognostic ratios for each level were obtained, with respect to every other level, according to possible changes in the explanatory variables considered. Certain personality factors are associated with one or more dimensions of burnout syndrome. Thus, nurses are more likely to develop high levels of burnout if they present high levels of neuroticism and low levels of friendliness and responsibility. Further research in this field is needed to confirm and extend these findings.
Understanding User Practices When Drawing up Requirements—The Case of Designing Assistive Devices for Mobility
Authors: Holtkamp Fred C. , Wouters Eveline J.M. , Verkerk Maarten J.
Published: January 2019

Patients with orthopedic problems often use assistive devices, e.g., ankle-foot orthoses and therapeutic footwear, to support their mobility. However, many users are not satisfied with their devices or do not use them at all, resulting in a decrease of quality of life. It has been shown that a main cause for dissatisfaction and non-use lies in the process of drawing up requirements. It appears that orthopedic engineers have too little insight in the different areas of life of patients leading to deficient design requirements. In this article a general approach—the so-called Triple I model—is presented to understand the different areas of life of patients. This model offers, in line with and directed by the intention of the International Classification of Functioning, Disability and Health (ICF) model three perspectives or ‘pairs of lenses’ to analyze these areas of life: the identity, the interests of key stakeholders or social actors, and the underlying societal ideals. The Triple I model is elaborated for assistive devices and offers an associated methodology to orthopedic engineers to systematically map the different areas of life of patients, to understand the requirements for every area, and to explore the conditions. In case of assistive devices five different areas of life have to be investigated: daily living at home, work, transport, social and spiritual activities, sport and leisure.
Household Food Insecurity Narrows the Sex Gap in Five Adverse Mental Health Outcomes among Canadian Adults
Authors: Jessiman-Perreault Geneviève , McIntyre Lynn
Published: January 2019

The sex gap (i.e., the significant difference in an outcome between men and women) in the occurrence of a variety of mental health conditions has been well documented. Household food insecurity has also repeatedly been found to be associated with a variety of poor mental health outcomes. Although both sex and household food insecurity have received attention individually, rarely have they been examined together to explore whether or how these indicators of two social locations interact to impact common mental health outcomes. Using a pooled sample (N = 302,683) of the Canadian Community Health Survey (2005–2012), we test whether sex modifies the relationship between household food insecurity assessed by the Household Food Security Survey Module and five adverse mental health outcomes, controlling for confounding covariates. Although the sex gap was observed among food secure men versus women, males and females reporting any level of food insecurity were equally likely to report adverse mental health outcomes, compared with those reporting food security. Therefore, household food insecurity seems to narrow the sex gap on five adverse mental health outcomes.
Bibliometric Analysis of Electronic Cigarette Publications: 2003–2018
Authors: Briganti Michael , Delnevo Cristine D. , Brown Leanne , Hastings Shirin E. , Steinberg Michael B.
Published: January 2019

Electronic cigarettes are at the center of a public health policy debate which leverages scientific publications. This study characterizes e-cigarette publication trends over the past 15 years via a bibliometric analysis. Scopus was searched for “electronic cigarette”, “e-cig”, “e-cigarette”, “vape”, “vaping”, “juul”, or “electronic nicotine delivery system” between 2003–2018. Data included Hirsch index, document type and frequency, and publications by institution, journal, and country. VOSviewer was used to visualize authorship network maps. A total of 4490 e-cigarette publications were identified, most (62.8%) being articles. After 2009, the annual growth rate for e-cigarette publications was the largest in 2014. The annual growth rate was nearly flat in 2017 but increased in 2018. The U.S. produced 51.6% of publications. Annual National Institutes of Health NIH funding for tobacco research mapped closely with the annual volume of e-cigarette publications. Author network analyses illustrated investigator collaborative patterns. The frequency of e-cigarette publications increased significantly in the past decade. A strong relationship of NIH funding for tobacco research and e-cigarette publications demonstrates the importance of e-cigarettes in tobacco research.
Interventional Effects of Weight-Loss Policy in a Healthy City among Participants with Metabolic Syndrome
Authors: Tai Hsu-Chih , Tzeng I-Shiang , Liang Yen-Ching , Liao Hsiu-Hui , Su Chun-Hsien , Kung Woon-Man
Published: January 2019

This study aimed to establish a friendly environment of active living and healthy eating for citizens while promoting and increasing knowledge of healthy exercise. Acquisition of physical activity skills and citizens’ lifestyle changes result in reduction in rates of obesity and deaths related to underlying metabolism syndrome. This study used a non-experimental cross-sectional design to survey residents living in Taiwan’s rural Miaoli County. The inclusion criterion was positive screening for metabolic syndrome. In total, 2068 participants were recruited, and 1886 questionnaires (91.2%) were completed. An organization-spreading strategy and home convenient Lifestyles of Health and Sustainability tactic were applied to the assessment, promotion, evaluation, and planning of the project via an obesity-causing environmental scan, oriental synergy aerobics, acupuncture points massage, guide books, broadcasting, town-based “shape-it-station”, and a vending cart created to facilitate the acquisition of healthy foods. After the intervention, results revealed that health condition, regular exercise habits, diet behavior, metabolic syndrome cognition, and body weight became better than before. Appropriate promotion of healthy cities through public health measures may effectively reduce the threat of death due to metabolic syndrome, which in turn reduces overall, and represents successful control of a typical non-communicable disease.
Discovery of Food-Derived Dipeptidyl Peptidase IV Inhibitory Peptides: A Review
Authors: Liu Rui , Cheng Jianming , Wu Hao
Published: January 2019

Diabetes is a chronic metabolic disorder which leads to high blood sugar levels over a prolonged period. Type 2 diabetes mellitus (T2DM) is the most common form of diabetes and results from the body’s ineffective use of insulin. Over ten dipeptidyl peptidase IV (DPP-IV) inhibitory drugs have been developed and marketed around the world in the past decade. However, owing to the reported adverse effects of the synthetic DPP-IV inhibitors, attempts have been made to find DPP-IV inhibitors from natural sources. Food-derived components, such as protein hydrolysates (peptides), have been suggested as potential DPP-IV inhibitors which can help manage blood glucose levels. This review focuses on the methods of discovery of food-derived DPP-IV inhibitory peptides, including fractionation and purification approaches, in silico analysis methods, in vivo studies, and the bioavailability of these food-derived peptides. Moreover, food-derived DPP-IV inhibitory peptides discovered during this decade are listed and distributed in a 3D scatter plot graph based on their IC50, molecular weight, and grand average of hydropathicity values, which can help us to understand the relationship between the features of the peptides and their activities.
Maternal Protein Restriction Differentially Alters the Expression of AQP1, AQP9 and VEGFr-2 in the Epididymis of Rat Offspring
Authors: Cavariani Marilia Martins , de Mello Santos Talita , Pereira Dhrielly Natalia , de Almeida Chuffa Luiz Gustavo , Felipe Pinheiro Patricia Fernanda , Scarano Wellerson Rodrigo , Domeniconi Raquel Fantin
Published: January 2019

Background: Maternal protein restriction causes sperm alterations in the offspring, most of which are associated with epididymal functions. Because fluid reabsorption/secretion dynamics in the epididymal environment play important roles in the process of sperm maturation and concentration, we investigated the effects of maternal protein restriction on the expression of aquaporins (AQP1 and AQP9), vascular endothelial growth factor (VEGFa), and its receptor VEGFr-2 in different stages of postnatal epididymal development. Methods: Pregnant rats were divided into groups that received normoprotein (17% protein) and low-protein diets (6% protein) during gestation and lactation. After weaning, male rats only received the standard diet and were euthanized at the predetermined ages of 21, 44 and 120 days. Results: Maternal protein restriction decreased AQP1 and AQP9 expression in the initial segment and caput epididymis compared to the increased expression of these proteins observed in the corpus and cauda at all ages. Although protein restriction reduced the microvasculature density (MVD) on postnatal day (PND) 21 and 44, the MVD was unaltered on PND 120. Conclusions: Maternal protein restriction changed the structure or function of the offspring’s epididymis, specifically by affecting fluid dynamics and vasculogenesis in important stages of epididymis development.
Cellulose-starch Hybrid Films Plasticized by Aqueous ZnCl2 Solution
Authors: Shang Xiaoqin , Jiang Huihua , Wang Qingling , Liu Peng , Xie Fengwei
Published: January 2019

Starch and cellulose are two typical natural polymers from plants that have similar chemical structures. The blending of these two biopolymers for materials development is an interesting topic, although how their molecular interactions could influence the conformation and properties of the resultant materials has not been studied extensively. Herein, the rheological properties of cellulose/starch/ZnCl2 solutions were studied, and the structures and properties of cellulose-starch hybrid films were characterized. The rheological study shows that compared with starch (containing mostly amylose), cellulose contributed more to the solution’s viscosity and has a stronger shear-thinning behavior. A comparison between the experimental and calculated zero-shear-rate viscosities indicates that compact complexes (interfacial interactions) formed between cellulose and starch with ≤50 wt % cellulose content, whereas a loose structure (phase separation) existed with ≥70 wt % cellulose content. For starch-rich hybrid films prepared by compression molding, less than 7 wt % of cellulose was found to improve the mechanical properties despite the reduced crystallinity of the starch; for cellulose-rich hybrid films, a higher content of starch reduced the material properties, although the chemical interactions were not apparently influenced. It is concluded that the mechanical properties of biopolymer films were mainly affected by the structural conformation, as indicated by the rheological results.
Epitope Mapping Immunoassay Analysis of the Interaction between β-Amyloid and Fibrinogen
Authors: Van Giau Vo , An Seong Soo A.
Published: January 2019

The vast majority of patients with Alzheimer’s disease (AD) suffer from impaired cerebral circulation. Substantial evidence indicates that fibrinogen (Fbg) and fibrin clot formation play an important role in this circulatory dysfunction in AD. Fbg interacts with β-amyloid (1-42) (Aβ), forming plasmin-resistant abnormal blood clots, and increased fibrin deposition has been discovered in the brains of AD patients and mouse models. In this study, biochemical approaches and the epitope mapping immunoassay were employed to characterize binding epitopes within the Fbg and complementary epitopes in Aβ. We discovered the Aβ5–25 peptide as the most critical region for the interaction, which can be inhibited by specific monoclonal and polyclonal antibodies against the central region of Aβ. Aβ binding to Fbg may block plasmin-mediated fibrin cleavage at this site, resulting in the generation of increased levels of plasmin-resistant fibrin degradation fragments. Our study elucidates the Aβ–Fbg interaction that may involve the mechanism by which Aβ–Fbg binding delays fibrinolysis by plasmin, providing valuable information in the development of therapeutic approaches for AD.
Exploring the Importance of Entities in Semantic Ranking
Authors: Li Zhenyang , Xu Guangluan , Liang Xiao , Li Feng , Wang Lei , Zhang Daobing
Published: January 2019

In recent years, entity-based ranking models have led to exciting breakthroughs in the research of information retrieval. Compared with traditional retrieval models, entity-based representation enables a better understanding of queries and documents. However, the existing entity-based models neglect the importance of entities in a document. This paper attempts to explore the effects of the importance of entities in a document. Specifically, the dataset analysis is conducted which verifies the correlation between the importance of entities in a document and document ranking. Then, this paper enhances two entity-based models—toy model and Explicit Semantic Ranking model (ESR)—by considering the importance of entities. In contrast to the existing models, the enhanced models assign the weights of entities according to their importance. Experimental results show that the enhanced toy model and ESR can outperform the two baselines by as much as 4.57% and 2.74% on NDCG@20 respectively, and further experiments reveal that the strength of the enhanced models is more evident on long queries and the queries where ESR fails, confirming the effectiveness of taking the importance of entities into account.
Magnetoelastic Effect Detection with the Usage of Eddy Current Tomography
Authors: Nowak Paweł
Published: January 2019

The possibility of application of the eddy current tomography setup to measure the small permeability variations caused by magnetoelastic effect was presented. A ferromagnetic steel sample was prepared for applying wall stresses and measured for 30 MPa stresses. The Finite Element Method (FEM) was utilized to conduct numerical forward tomography transformation for samples of known permeability. Developed forward tomography transformation was applied for single variable inverse tomography transformation, utilized for determining magnetic permeability. This confirmed the possibility of the application of eddy current tomography for quantitative measurements of magnetoelastic effect in samples of known geometry.
Investigation of Grain Refinement Mechanism of Nickel Single Crystal during High Pressure Torsion by Crystal Plasticity Modeling
Authors: Wei Peitang , Zhou Hao , Liu Huaiju , Zhu Caichao , Wang Wei , Deng Guanyu
Published: January 2019

The excellent properties of ultra-fine grained (UFG) materials are relevant to substantial grain refinement and the corresponding induced small grains delineated by high-angle grain boundaries. The present study aims to understand the grain refinement mechanism by examining the nickel single crystal processed by high pressure torsion (HPT), a severe plastic deformation method to produce UFG materials based upon crystal plasticity finite element (CPFEM) simulations. The predicted grain maps by the developed CPFEM model are capable of capturing the prominent characteristics associated with grain refinement in HPT. The evolution of the orientation of structural elements and the rotations of crystal lattices during the HPT process of the detected differently oriented grains are extensively examined. It has been found that there are mainly two intrinsic origins of lattice rotation which cause the initial single crystal to subdivide. The correlation between the crystallographic orientation changes and lattice rotations with the grain fragmentation are analyzed and discussed in detail based on the theory of crystal plasticity.
Experimental Study on Erosion–Corrosion of TP140 Casing Steel and 13Cr Tubing Steel in Gas–Solid and Liquid–Solid Jet Flows Containing 2 wt % NaCl
Authors: Cheng Jiarui , Li Zhen , Zhang Ningsheng , Dou Yihua , Cui Lu
Published: January 2019

To study the erosion–corrosion characteristics of TP140 casing steel and 13Cr tubing steel in oil fields, we performed gas–solid and liquid–solid jet flow experiments to control particle addition, jet angle, and flow velocity and measure erosion and corrosion components. Meanwhile, we used a standard three-electrode system to study the changes in electrochemical parameters on a metal surface in a two-phase flow containing 2 wt % NaCl. Results showed that erosion is mainly dominated by the flow velocities and impact angles of particles, and corrosion rate is mainly affected by liquid flow rate. The erosion rates of the two materials increase with flow velocity, and the critical angle of maximum erosion rate exists. Meanwhile, flow velocity growth increases the current density on the TP140 surface while reducing the corrosion potential of 13Cr, but the effect of the angle on the two parameters is relatively small. The uniform corrosion of TP140 increases the erosion rate in the range of 10–20%, and the pitting of 13Cr increases the erosion rate in the range of 30–90%, indicating that the interaction between the erosion and corrosion of stainless steel is obvious.
A Further Extension for Ramanujan’s Beta Integral and Applications
Authors: Xi Gao-Wen , Luo Qiu-Ming
Published: January 2019

In 1915, Ramanujan stated the following formula ∫ 0 ∞ t x − 1 ( − a t ; q ) ∞ ( − t ; q ) ∞ d t = π sin π x ( q 1 − x , a ; q ) ∞ ( q , a q − x ; q ) ∞ , where 0 < q < 1 , x > 0 , and 0 < a < q x . The above formula is called Ramanujan’s beta integral. In this paper, by using q-exponential operator, we further extend Ramanujan’s beta integral. As some applications, we obtain some new integral formulas of Ramanujan and also show some new representation with gamma functions and q-gamma functions.
Isolation and Potential Biological Applications of Haloaryl Secondary Metabolites from Macroalgae
Authors: Jesus Ana , Correia-da-Silva Marta , Afonso Carlos , Pinto Madalena , Cidade Honorina
Published: January 2019

Macroalgae have been reported as an important source of halogenated aromatic secondary metabolites, being the majority of these derivatives isolated from red algae. Halophenols and haloindoles are the most common haloaryl secondary metabolites isolated from these marine organisms. Nevertheless, some halogenated aromatic sesquiterpenes and naphthalene derivatives have also been isolated. Most of these secondary metabolites showed interesting biological activities, such as antitumor, antimicrobial, antidiabetic, and antioxidant. This review describes in a systematic way the distribution and natural occurrence of halogenated aromatic secondary metabolites from extracts of red, brown, and green algae, as well as biological activities reported for these compounds.
Three-Dimensional Au/Holey-Graphene as Efficient Electrochemical Interface for Simultaneous Determination of Ascorbic Acid, Dopamine and Uric Acid
Authors: Jing Aihua , Liang Gaofeng , Yuan Yixin , Feng Wenpo
Published: January 2019

The quantification of ascorbic acid (AA), dopamine (DA), and uric acid (UA) has been an important area of research, as these molecules’ determination directly corresponds to the diagnosis and control of diseases of nerve and brain physiology. In our research, graphene oxide (GO) with nano pores deposited with gold nanoparticles were self-assembled to form three-dimensional (3D) Au/holey-graphene oxide (Au/HGO) composite structures. The as-prepared 3DAu/HGO composite structures were characterized for their structures by X-ray diffraction, Raman spectrum, scanning electron microscopy, and transmission electron microscopy coupled with cyclic voltammograms. Finally, the proposed 3DAu/HGO displayed high sensitivity, excellent electron transport properties, and selectivity for the simultaneous electrochemical determination of AA, DA and UA with linear response ranges of 1.0–500 μM, 0.01–50 μM and 0.05–50 μM respectively. This finding paves the way for graphene applications as a biosensor for detecting three analytes in human serum.
Approaches to the Structure-Based Design of Antivirulence Drugs: Therapeutics for the Post-Antibiotic Era
Authors: Neville Nolan , Jia Zongchao
Published: January 2019

The alarming rise of multidrug-resistant bacterial strains, coupled with decades of stagnation in the field of antibiotic development, necessitates exploration of new therapeutic approaches to treat bacterial infections. Targeting bacterial virulence is an attractive alternative to traditional antibiotics in that this approach disarms pathogens that cause human diseases, without placing immediate selective pressure on the target bacterium or harming commensal species. The growing number of validated virulence protein targets for which structural information has been obtained, along with advances in computational power and screening algorithms, make the rational design of antivirulence drugs a promising avenue to explore. Here, we review the principles of structure-based drug design and the exciting opportunities this technique presents for antivirulence drug discovery.
Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media
Authors: Tartaglia Angela , Locatelli Marcello , Kabir Abuzar , Furton Kenneth G. , Macerola Daniela , Sperandio Elena , Piccolantonio Silvia , Ulusoy Halil I. , Maroni Fabio , Bruni Pantaleone , Croce Fausto , Samanidou Victoria F.
Published: January 2019

This paper reports the performance comparison between the exhaustive and equilibrium extraction using classical Avantor C18 solid phase extraction (SPE) sorbent, hydrophilic-lipophilic balance (HLB) SPE sorbent, Sep-Pak C18 SPE sorbent, novel sol-gel Carbowax 20M (sol-gel CW 20M) SPE sorbent, and sol-gel CW 20M coated fabric phase sorptive extraction (FPSE) media for the simultaneous extraction and analysis of three inflammatory bowel disease (IBD) drugs that possess logP values (polarity) ranging from 1.66 for cortisone, 2.30 for ciprofloxacin, and 2.92 for sulfasalazine. Both the commercial SPE phases and in-house synthesized sol-gel CW 20M SPE phases were loaded in SPE cartridges and the extractions were carried out under an exhaustive extraction mode. FPSE was carried out under an equilibrium extraction mode. The drug compounds were resolved using a Luna C18 column (250 mm × 4.6 mm; 5 μm particle size) in gradient elution mode within 20 min and the method was validated in compliance with international guidelines for the bioanalytical method validation. Novel in-house synthesized and loaded sol-gel CW 20M SPE sorbent cartridges were characterized in terms of their extraction capability, breakthrough volume, retention volume, hold-up volume, number of the theoretical plate, and the retention factor.
Enhanced NADH Metabolism Involves Colistin-Induced Killing of Bacillus subtilis and Paenibacillus polymyxa
Authors: Yu Zhiliang , Zhu Yuyi , Fu Jianv , Qiu Juanping , Yin Jianhua
Published: January 2019

The commonly believed mechanism of colistin against Gram-negative bacteria is to cause cell membrane lysis, whereas the mechanism of colistin against Gram-positive bacteria is extremely fragmented. In this study, we found that colistin treatment on Bacillus subtilis WB800, Paenibacillus polymyxa C12 and Paenibacillus polymyxa ATCC842 enhances not only the activities of α-ketoglutaric dehydrogenase and malate dehydrogenase in tricarboxylic acid (TCA) cycle, but also the relative expression levels of their encoding genes. Additionally, the oxaloacetate concentration also increases. Interestingly, the analysis of the relative expression of genes specific for respiratory chain showed that colistin treatment stimulates the respiratory chain in Gram-positive bacteria. Accordingly, the NAD+/NADH ratio increases and the oxidative level is then boosted up. As a result, the intensive oxidative damages are induced in Gram-positive bacteria and cells are killed. Notably, both rotenone and oligomycin, respectively, inhibiting NADH dehydrogenase and phosphorylation on respiratory chain can downgrade oxidative stress formation, thus alleviating the colistin-induced killing of Gram-positive cells. Besides, thiourea-based scavenging for reactive oxygen species also rescues the colistin-subjected cells. These data collectively demonstrate that colistin stimulates both TCA cycle and respiratory chain in Gram-positive bacteria, leading to the enhancement of NADH metabolism and resulting in the generation of oxidative damages in Gram-positive cells. Our studies provide a better understanding of antibacterial mechanism of colistin against Gram-positive bacteria, which is important for knowledge on bacterial resistance to colistin happening via the inhibition of respiratory chain and manipulation of its production.
High Performance Liquid Chromatography Determination and Optimization of the Extraction Process for the Total Alkaloids from Traditional Herb Stephania cepharantha Hayata
Authors: Xiao Jiao , Pan Yingni , Zhang Lin , Wang Xia , Han Yueqing , Sun Lu , Chen Gang , Li Ning
Published: January 2019

Stephania cepharantha Hayata is a traditional Chinese herbal medicine used to treat lung cancer, and its alkaloids, especially cepharanthine (CEP), were reported to be its effective ingredients. Therefore, the extraction of potential antitumor ingredients from the plant was of interest. We first explored the optimized solvent extraction of antitumor agents from S. cepharantha Hayata guided by an in vitro antitumor activity assay. The solvent for extraction and its concentration, the liquid to material ratio, extraction duration, particle size, macerating time, and the frequency of extraction were investigated using a single-factor experiment. An orthogonal design (L9, 34) was constructed to determine the suitable extraction conditions. The crude extract was then purified sequentially by macroporous adsorption resins (MR) for the enrichment of CEP. Under these optimal conditions, the yield of total alkaloids in the herbs was 3.4%, whereas the CEP content was 2.9%. Total alkaloids exhibited significant anti-proliferative activities in the A549 cell line. Our study provides means for the further development and use of the antitumor components from S. cepharantha, which has potential for application in the pharmaceutical industry.
Effect of Surface Cleaning Regimen on Glass Ceramic Bond Strength
Authors: Lapinska Barbara , Rogowski Jacek , Nowak Joanna , Nissan Joseph , Sokolowski Jerzy , Lukomska-Szymanska Monika
Published: January 2019

This study investigated the effect of saliva contamination on chemical changes of ceramic surface as well as the influence of saliva cleaning methods on ceramic-resin bond strength. Saliva was used to contaminate leucite (LGC) and lithium disilicate (LDGC) glass ceramic surfaces. The following cleaning methods were tested: water spray, cleaning with orthophosphoric acid, universal cleaning paste, ultrasonic cleaning with water, re-etching with hydrofluoric acid. Non-contaminated ceramic sample served as control. Chemical analysis of ceramic surfaces was performed using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Shear bond strength (SBS) of ceramics to resin material was tested after 24-hour water storage and after thermocycling. The most effective cleaning method of saliva-contaminated ceramic surface was cleaning LGC surface with orthophosphoric acid or re-etching the LDGC surface with hydrofluoric acid. The application of the following methods resulted in obtaining reliable bond strength.
Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
Authors: Semreen Mohammad H. , Soliman Sameh S. M. , Saeed Balsam Q. , Alqarihi Abdullah , Uppuluri Priya , Ibrahim Ashraf S.
Published: January 2019

Candida auris, a newly-emerging Candida species, is a serious global health threat due to its multi-drug resistant pattern, difficulty to diagnose, and the high mortality associated with its invasive and bloodstream infections. Unlike C. albicans, and C. dubliniensis which can form true hyphae, C. auris grows as yeast or pseudohyphae and is capable of developing biofilms. The reasons for the inability of C. auris to form true hyphae are currently unknown. Metabolites secreted by microorganisms, including Candida, are known as important factors in controlling morphogenesis and pathogenesis. Metabolic profiling of C. auris and C. albicans cultures was performed using gas chromatography–mass spectrometry (GC–MS). Compared to C. albicans, C. auris secreted several hyphae-inhibiting metabolites, including phenylethyl, benzyl and isoamyl alcohols. Furthermore, a biofilm-forming metabolite—tyrosol—was identified. On the other hand, several other biomarkers identified from C. auris but not from C. albicans cultures may be produced by the organism to overcome the host immune system or control fungal adaptations, and hence ease its invasion and infections. The results from this study are considered as the first identification of C. auris metabolic activities as a step forward to understand its virulence mechanisms.
HMPA-Catalyzed Transfer Hydrogenation of 3-Carbonyl Pyridines and Other N-Heteroarenes with Trichlorosilane
Authors: Fu Yun , Sun Jian
Published: January 2019

A method for the HMPA (hexamethylphosphoric triamide)-catalyzed metal-free transfer hydrogenation of pyridines has been developed. The functional group tolerance of the existing reaction conditions provides easy access to various piperidines with ester or ketone groups at the C-3 site. The suitability of this method for the reduction of other N-heteroarenes has also been demonstrated. Thirty-three examples of different substrates have been reduced to designed products with 45–96% yields.
pKa Determination of a Histidine Residue in a Short Peptide Using Raman Spectroscopy
Authors: Pogostin Brett H. , Malmendal Anders , Londergan Casey H. , Åkerfeldt Karin S.
Published: January 2019

Determining the pKa of key functional groups is critical to understanding the pH-dependent behavior of biological proteins and peptide-based biomaterials. Traditionally, 1H NMR spectroscopy has been used to determine the pKa of amino acids; however, for larger molecules and aggregating systems, this method can be practically impossible. Previous studies concluded that the C-D stretches in Raman are a useful alternative for determining the pKa of histidine residues. In this study, we report on the Raman application of the C2-D probe on histidine’s imidazole side chain to determining the pKa of histidine in a short peptide sequence. The pKa of the tripeptide was found via difference Raman spectroscopy to be 6.82, and this value was independently confirmed via 1H NMR spectroscopy on the same peptide. The C2-D probe was also compared to other Raman reporters of the protonation state of histidine and was determined to be more sensitive and reliable than other protonation-dependent signals. The C2-D Raman probe expands the tool box available to chemists interested in directly interrogating the pKa’s of histidine-containing peptide and protein systems.
Determination of Selected Priority Pesticides in High Water Fruits and Vegetables by Modified QuEChERS and GC-ECD with GC-MS/MS Confirmation
Authors: Tankiewicz Maciej
Published: January 2019

A modified quick, easy, cheap, efficient, rugged and safe (QuEChERS) method coupled to gas chromatography with electron capture detector (GC-ECD) was developed for simultaneous determination of selected electronegative pesticides in fruits and vegetables with high water content. The chosen compounds are commonly detected in fruit and vegetable crops, and some of their metabolites have even been found in human urine. In addition, some of them are known or suspected carcinogens according to the International Agency for Research of Cancer. Extraction and clean up parameters were optimized, thus the original QuEChERS method was modified to decrease solvent usage, in accordance with ‘green chemistry’ principles. The proposed methodology was validated in terms of selectivity, specificity, linearity, precision and accuracy. The obtained limits of detection (LODs) for all investigated pesticides ranged from 5.6 µg·kg−1 to 15 µg·kg−1 and limits of quantification (LOQs) from 17 µg·kg−1 to 45 µg·kg−1. The obtained data demonstrated the good reproducibility and stability of the procedure in the tested concentration range up to 10 mg·kg−1, with relative standard deviations (RSDs) lower than 10%. Recoveries for spiked pear samples at LOQ level for each pesticide were from 90% to 107% with RSDs lower than 9.6%. The suitability of the developed procedure was tested on various fruit and vegetable samples available on the market at different seasons. The proposed methodology is applicable for detection and monitoring of selected pesticides not only in fruits and vegetables with high water content, but also in samples containing large amounts of pigments and dyes.
Investigations into the Performance of a Novel Pocket-Sized Near-Infrared Spectrometer for Cheese Analysis
Authors: Wiedemair Verena , Langore Dominik , Garsleitner Roman , Dillinger Klaus , Huck Christian
Published: January 2019

The performance of a newly developed pocket-sized near-infrared (NIR) spectrometer was investigated by analysing 46 cheese samples for their water and fat content, and comparing results with a benchtop NIR device. Additionally, the automated data analysis of the pocket-sized spectrometer and its cloud-based data analysis software, designed for laypeople, was put to the test by comparing performances to a highly sophisticated multivariate data analysis software. All developed partial least squares regression (PLS-R) models yield a coefficient of determination (R2) of over 0.9, indicating high correlation between spectra and reference data for both spectrometers and all data analysis routes taken. In general, the analysis of grated cheese yields better results than whole pieces of cheese. Additionally, the ratios of performance to deviation (RPDs) and standard errors of prediction (SEPs) suggest that the performance of the pocket-sized spectrometer is comparable to the benchtop device. Small improvements are observable, when using sophisticated data analysis software, instead of automated tools.
New Poly(Ionic Liquid) Based Fiber for Determination of Oxytetracycline in Milk Samples by Application of SPME-CE Technique
Authors: Ferreira T. Alexandra , Flores-Aguilar J. Francisco , Santos Eva M. , Rodriguez Jose A. , Ibarra Israel S.
Published: January 2019

In this work, a procedure using solid phase microextraction in combination with capillary electrophoresis was developed for the determination of oxytetracycline in milk samples. The method involves the synthesis of poly(1-allyl-3-methyl imidazolium) chloride film on a stainless-steel bar via electropolymerization and its use as an adsorbent for oxytetracycline (OT) by an ionic exchange mechanism. The coated fiber is then immersed in milk samples for retention of oxytetracycline residues, followed by elution, drying, and reconstitution before analysis with capillary electrophoresis. The proposed method achieves a limit of detection of 70 µg L−1 with adequate precision and uncertainty, making this methodology appropriate for the determination of OT in milk samples. The method was applied to the pre-concentration and quantification of oxytetracycline in ten commercial milk samples. Two tested samples were positive for the presence of oxytetracycline but the concentration was below the maximum residue limit according to the international normative standard. The proposed methodology was evaluated according to the Eco-Scale approach, and the total score of 51 indicated that the methodology proposed is both green and acceptable despite the multi-stage character. SPME-CE methodology allows us to perform the sample pre-treatment and determination of OT in an effective and greener way, decreasing the number of steps during the analysis and the generation of waste.
The End of Islands: Drawing Insight from Revelation to Respond to Prisoner Radicalization and Apocalyptically-Oriented Terrorism
Authors: Trinka Eric M.
Published: January 2019

This paper is an exploratory exercise in practical theology and interfaith engagement that probes the conceptual frameworks of insularity (Islandness) and incarceration in Revelation in order to address the related problems of prisoner radicalization and apocalyptically-oriented terrorism. It offers an experimental reading of Revelation performed through the lenses of island studies, criminology, and research on prisoner radicalization. While inmates may adopt a range of religious dispositions, Islam is the fastest-growing religion in U.S. prisons. Moreover, even though Islam is not inherently violent nor are Muslims more predisposed to radical behaviors than other religious groups, some forms of prison Islam promote brutal apocalyptic worldviews and incite adherents to violence. This paper examines the place Revelation maintains in Islamic apocalyptic thought and asks how Revelation can assist in the fight against radicalization among an increasingly Muslim inmate population. Islands, prisons, and prisoners share a robust set of real and metaphorical relationships. The islanded nature of John’s experience provides a valuable point of access for incarcerated readers who find themselves in similarly marginalized social locations where radical readings are more likely to occur. Reading the insular and carceral elements of Revelation in tandem with these bodies of research is instructive for cultivating constructive responses to the present set of problems. It is argued that while Revelation can be a potential source of violent ideologies, it also offers its own internal checks against violent enactments. John’s vision culminates in the end of islands (Rev 21:1). The overarching goal of this essay is to ask how we might point readers in physically and ideologically insular environments toward constructive interpretations of apocalyptica in order to stem the persistent problem of violent radicalization.
The Dreamer and Their Authenticity in the Zhuangzi
Authors: Bonar Barbara Aniela
Published: January 2019

In this paper, I explain the problem of the dreamer in the Zhuangzi. I aim to show that no difference exists between dreaming states and waking states because we have a fluctual relationship with these two stages. In both, “we are dreaming.” Put another way, from a psychoanalytical point of view, one stage penetrates the other and vice versa. The difference between dreaming and non-dreaming disappears because dreaming is a structural process. Also, from a psychoanalytical perspective, all confirmations and negations about dreams and non-dreams leads to one point: the being, or rather the becoming, of the subject. How does this solve the problem of the True Person/True Human Being (zhenren真人)? Does such a person have dreams or not? Does the True Person sleep without dreams, as we find in the Zhuangzi? From a psychoanalytic perspective, this is not possible. To prove this, I will present few passages from the Zhuangzi and offer a psychoanalytic explanation of them based on Jacques Lacan’s theory of the fantasy and desire.
Political Economy as a Test of Modern Judaism
Authors: Brody Samuel Hayim
Published: January 2019

According to a common narrative, Jews entered the modern world at a steep price. From an autonomous corporation, ruling themselves internally according to their own standards and law, Judaism became a “religion,” divested of political power and responsible only for the internal sphere of “faith” or belief. The failure of this project, in turn, gave rise to the sharp split between Jewish nationalism and religion-based conceptions of Judaism. Many modern Jewish thinkers sought to resolve this antinomy by imagining ways for Judaism to once again form the basis of a “complete life”. This essay seeks to challenge this narrative by examining the extent to which economics, another one of the “spheres” emerging together with modernity and often considered under the same broadly Weberian process of rationalization, ever truly formed part of the holistic, self-contained Jewish autonomous life for which modern thinkers expressed so much nostalgia. It will argue that rather than forming part of the internal world of Judaism and then being fragmented outward into a separate sphere under the pressure of modernity, the “economic sphere” was imagined and defined for the first time in modernity, and projected backwards into earlier eras. This projection was then taken as proof of Judaism’s ability to “be about everything,” whether in a religious or nationalist idiom.
A New Method for Large-Scale Landslide Classification from Satellite Radar
Authors: Burrows Katy , Walters Richard J. , Milledge David , Spaans Karsten , Densmore Alexander L.
Published: January 2019

Following a large continental earthquake, information on the spatial distribution of triggered landslides is required as quickly as possible for use in emergency response coordination. Synthetic Aperture Radar (SAR) methods have the potential to overcome variability in weather conditions, which often causes delays of days or weeks when mapping landslides using optical satellite imagery. Here we test landslide classifiers based on SAR coherence, which is estimated from the similarity in phase change in time between small ensembles of pixels. We test two existing SAR-coherence-based landslide classifiers against an independent inventory of landslides triggered following the Mw 7.8 Gorkha, Nepal earthquake, and present and test a new method, which uses a classifier based on coherence calculated from ensembles of neighbouring pixels and coherence calculated from a more dispersed ensemble of ‘sibling’ pixels. Using Receiver Operating Characteristic analysis, we show that none of these three SAR-coherence-based landslide classification methods are suitable for mapping individual landslides on a pixel-by-pixel basis. However, they show potential in generating lower-resolution density maps, which are used by emergency responders following an earthquake to coordinate large-scale operations and identify priority areas. The new method we present outperforms existing methods when tested at these lower resolutions, suggesting that it may be able to provide useful and rapid information on landslide distributions following major continental earthquakes.