bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved identification and tracking of three-dimensional eddies in the Southern Ocean utilizing 3D-U-Res-Net
Authors: Liu Chang , Lin Xiayan , Xu Guangjun , Han Guoqing , Liu Yu
Published: November 2024

Oceanic mesoscale eddies are prevalent throughout the global ocean, playing a critical role in material and energy transport while significantly influencing climate change. Accurate characterization of their three-dimensional structures and movement is essential for a quantitative analysis of their transport processes. Traditional eddy detection algorithms have lower successful detection rate and with more limitations, so they fall short in the complex and dynamic ocean environment. The rising trend of applying artificial intelligence (AI) algorithms, due to their efficiency, precision, and automation, addresses this challenge. This study employs the 3D-U-Res-Net algorithm to identify the three-dimensional structures of mesoscale eddies in the Southern Ocean using GLORYS12V1 data from 2011 to 2020. A vector geometry-based eddy detection algorithm (VG) initially identified 1587292 eddy snapshots in the Southern Ocean (2011–2019), which were used for training the 3D-U-Res-Net algorithm. Data from 2020 served as the ground truth and validation set. The successful detection rate of 3D-U-Res-Net algorithm is 100%, which means that it identified all 135734 eddy snapshots from the VG dataset in 2020. For eddy tracking, the VG algorithm counted 18168 eddy tracks, whereas the 3D-U-Res-Net counted 18559, reflecting a 2.15% bias. To reduce uncertainty, eddies with lifespans shorter than two weeks were excluded. The average lifespans and traveling distances for eddies detected by the 3D-U-Res-Net (VG) algorithm were 29.35 (29.61) days and 77.78 (37.60) km, respectively, with the 3D-U-Res-Net identifying eddies with longer traveling distances. The mean radius of eddies detected by the VG algorithm was 43.16 km, while the 3D-U-Res-Net detected eddies with a mean radius of 43.74 km, a 0.58 km increase. We categorized eddies into four three-dimensional structures: bowl-shaped, cone-shaped, lens-shaped, and cylindrical. The VG algorithm identified these structures in proportions of 32%, 31%, 25%, and 12%, respectively, whereas the 3D-U-Res-Net algorithm found 19.48%, 19.58%, 0.04%, and 60.9%, respectively. The 3D-U-Res-Net identified more cylindrical eddies and was approximately ten times faster than the VG algorithm. Overall, this algorithm has good performance and higher efficiency. It is an attempt of using AI for oceanic research, and more works can be carried out in the future.
Assessment of GOCI-II satellite remote sensing products in Lake Taihu
Authors: Zhao Min , Li Huaming , Li Hao , Zhang Xuan , Ding Xiaosong , Gong Fang
Published: November 2024

Introduction

The Geostationary Ocean Color Imager-II (GOCI-II), launched on February 19, 2020, offers increased observation times throughout the day and higher spatial resolution compared to its predecessor, the Geostationary Ocean Color Imager (GOCI), launched in 2010. To ensure the reliability of GOCI-II data for practical applications, the accuracy of remote sensing products needs to be validated. This study uses in situ data from Lake Taihu for validation.

Methods

We assessed the accuracy of GOCI-II remote sensing products, including remote sensing reflectance derived using two atmospheric correction algorithms: ultraviolet (UV) and near-infrared (NIR). The study also evaluated the accuracy of derived parameters, such as chlorophyll-a (Chl-a) concentration, total suspended matter (TSM) concentration, and phytoplankton absorption coefficient (aph), based on these atmospheric correction algorithms. In situ measurements from Lake Taihu were used as ground truth data for validation.

Results

Our results revealed that the UV atmospheric correction algorithm provided higher accuracy in Lake Taihu compared to the NIR algorithm. The average absolute percentage deviations (APDs) for remote sensing reflectance across different bands were: 25.17% (412 nm), 29.69% (443 nm), 22.27% (490 nm), 19.38% (555 nm), 36.83% (660 nm), and 33.0% (680 nm). Compared to NIR-derived products, the UV algorithm showed improved accuracy for Chl-a concentration, TSM concentration, and aph, with reductions in APD values by 16.92%, 3.32%, and 10.91%, respectively. When applying UV correction, the 412 nm band performed better than the 380 nm band, likely due to a lower signal-to-noise ratio at 380 nm and smaller extrapolation errors at 412 nm.

Discussion

While the NIR algorithm is suitable for open ocean waters, the UV algorithm demonstrated higher accuracy in turbid environments such as Lake Taihu. Therefore, a combined UV-NIR atmospheric correction algorithm may be more effective for handling various types of water environments. Additionally, further research is needed to develop more suitable retrieval algorithms for Chl-a concentration and aph in eutrophic waters to improve accuracy.
Morphology and distribution of suspended particles during typhoon-induced algal bloom in the Pearl River Estuary
Authors: Lin Yaokun , Ye Leiping , Li Chao , Cui Yongsheng , Wu Jiaxue
Published: November 2024

Suspended particles, including plankton and clay minerals, are ubiquitous in aquatic environments. Understanding their characteristics is crucial for gaining insights into biogeochemical processes and accurately assessing material and element fluxes in coastal estuaries. Following the impact of Typhoon Cempaka on the Pearl River Estuary (PRE) in July 2021, we conducted field observations throughout various stages of the subsequent algal bloom, simultaneously capturing holographic images of particles alongside hydrographic data. We developed an innovative method to transform these images into datasets for deep learning object detection models, enabling advanced morphological analysis. This approach allowed for efficient identification and characterization of particle morphology and vertical distribution in coastal estuarine environments. Our study revealed substantial morphological and distributional differences in diatoms and aggregates in response to environmental changes throughout the stages of the typhoon-induced algal bloom. Specifically, elongated-curled diatoms tended to settle in the middle and bottom layers under turbulent mixing but remained concentrated in the surface phytoplankton layer under stratified conditions. In contrast, short-straight diatoms exhibited minimal sensitivity to physical dynamics, persisting in the surface layer across all conditions. We observed that aggregate morphology and distribution patterns correlated with physical dynamics intensity and diatom concentration. These findings accurately reflect particles’ natural states and underscore the potential of in situ particle morphology and distribution as indicators of environmental changes, highlighting the ecological significance of studying in situ particle functional traits. We recommend that future studies expand particle imaging across diverse conditions to deepen understanding of estuarine ecosystem evolution.
Vitamin K intake levels are associated with bone health in people aged over 50 years: a NHANES-based survey
Authors: Guo Jiankui , Zhou Ziqi , Gong Jie , Hu Wen , Liu Yuan
Published: November 2024

Background

Bone health is important for older adults, and vitamin K (VK) is central to regulating bone formation and promoting bone health. However, whether VK can reduce the risk of osteoporosis and bone loss is unclear. This study hypothesized that different levels of VK intake exert varying effects on bone health in people aged over 50 years.

Methods

Individuals aged above 50 years were recruited from the National Health and Nutrition Examination Survey. VK intake, based on 24-h dietary recall, was divided into three groups, namely the high, medium, and low groups, by sex and tertile. Weighted multiple logistic regression was used to investigate the effects of VK intake on the risk of osteoporosis and bone loss at the femoral neck, trochanter, intertrochanter, total femur, lumbar spine, and overall.

Results

This study included 5,075 individuals. Of them, 1,001 (18%) had osteoporosis (808 women, 83%) and 2,226 (46%) had osteopenia (1,076 women, 54%). Overall, a medium level of VK intake was associated with a reduced risk of bone loss. In women, medium- [odds ratio, OR (95% confidence interval, CI): 0.66(0.47, 0.93)] and high-level [OR (95% CI): 0.71(0.52, 0.98)] VK intake were associated with a decreased risk of osteoporosis. In contrast, only medium-level VK intake was associated with a reduced risk of bone loss [OR (95% CI): 0.58(0.41, 0.81)]. Similar results were obtained for the trochanter, intertrochanter, total femur, and lumbar spine. In men, only medium-level VK intake was associated with a reduced risk of bone loss at the femoral neck [OR (95% CI): 0.66(0.48, 0.90)], whereas high-level VK intake corresponded to a reduced risk of bone loss to the lumbar spine [OR (95% CI): 0.68(0.47, 0.99)]. Nonetheless, VK intake levels did not affect the risk of osteoporosis.

Conclusion

This study demonstrates sex- and bone-site-specific variations in the associations between VK intake levels and bone health in individuals aged over 50 years. Further large-scale cohort studies or randomized controlled trials are warranted to explore the effects of different VK intake levels on bone health in people regardless of their sex and bone site.
Advancements in aspiration catheter tip design for thrombectomy: a comprehensive patent review
Authors: Kortman Vera G. , Verberne Yinte , Atalla Mostafa A. , Sakes Aimée
Published: November 2024

Thrombus removal from the human body is facilitated through the utilization of aspiration catheters during minimally invasive thrombectomy procedures, where a pressure differential guides the targeted tissue through a flexible tubular medical instrument. In this paper, we present a patent analysis of thrombectomy aspiration catheter tip designs sourced from the EspaceNet database. Our findings reveal that enhancing the operability of aspiration catheters can be achieved by improving ease of positioning or suction capacity, whether through active or passive means. In terms of the former, both tip shape and flexibility play pivotal roles in maneuvering the distal end effectively. Variations in aspiration port characteristics, either distal-oriented or sideways-oriented, have the potential to enhance suction efficiency. In the active approach, aspects of positioning and suctioning are integrated into a single design, allowing for seamless transitions between configurations. While numerous design characteristics can coexist in a thrombectomy aspiration tip, a balance between flexibility and buckling resistance, as well as between maximizing aspiration lumen diameter and minimizing tip diameter, must be struck. This paper offers an insightful overview of existing thrombectomy aspiration tip designs, providing valuable inspiration for future innovations in this field.
Influence of specific tobacco endophytic Bacillus on tobacco leaf quality enhancement during fermentation
Authors: Wei Jinbin , Song Kai , Zang Zhipeng , Yang Hongjing , Gao Yuzhen , Zhang Jiandong , Wang Zhen , Liu Chen
Published: November 2024

Introduction

This study aimed to investigate the potential role of endophytic bacteria in tobacco leaves during the fermentation process to enhance the quality of tobacco.

Methods

We isolated 11 endophytic bacteria from fresh tobacco leaves and selected Bacillus halotolerans NS36 and Bacillus mycoides NS75 based on sensory evaluation, both of which significantly improved the sensory quality of tobacco leaves.

Results

Specifically, NS36 decreased offensive taste in tobacco leaves, while NS75 improved the quality by increasing the aroma. Chemical analysis revealed that fermentation with B. halotolerans NS36 significantly decreased the content of irritant compounds such as lignin, cellulose, starch, and pectin. In contrast, fermentation with B. mycoides NS75 reduced the content of cellulose, starch, and protein, while significantly increasing the content of Amadori compounds and glycosides. Through whole-genome sequencing, we predicted enzyme systems related to these chemical changes. B. halotolerans NS36 mainly secreted enzyme systems associated with the degradation of lignin, cellulose, starch, and pectin, thereby reducing irritants in tobacco leaves, diminishing unpleasant tastes, and achieving a more balanced sensory quality. B. mycoides NS75, on the other hand, secreted enzyme systems related to protein and glycoside hydrolysis, increasing Maillard reaction products and glycosylated compounds in tobacco leaves, thus enhancing the aroma quality and quantity.

Discussion

The findings of this study offer a new perspective for the tobacco industry, namely, the use of endophytic bacilli to improve the off-flavors and aroma of tobacco leaves, which could not only enhance the industrial applicability of tobacco leaves but also potentially strengthen the market competitiveness of products. These discoveries lay the foundation for further research and application, especially in the development of new biotechnologies to improve the quality of tobacco products.
Body mass index and fat influences the role of Bifidobacterium genus in lupus patients concerning fibrinogen levels
Authors: Chero-Sandoval Lourdes , Higuera-Gómez Andrea , Cuevas-Sierra Amanda , de Cuevillas Begoña , Castejón Raquel , Martínez-Urbistondo María , Mellor-Pita Susana , Moreno-Torres Víctor , de Luis Daniel , Martínez J. Alfredo
Published: November 2024

Introduction

Metabolic disorders and autoimmune diseases elicit distinct yet interconnected manifestations of inflammation, which may be boosted by an excess of body adiposity. The purpose of this investigation was to analyze anthropometric, biochemical, and inflammatory/coagulation variables concerning patients diagnosed with systemic lupus erythematosus (SLE) exploiting low-grade metabolic inflammation (MI), as reference.

Methods

A population stratification by body mass index (BMI), allowed to assess the impact of adiposity on the putative role of gut microbiota composition on coagulation markers. A total of 127 participants with MI and SLE were categorized into two main groups based on their BMI, following WHO criteria: a low BMI group (<30 kg/m2) and a high BMI group (≥30 kg/m2). Each group included recorded data on demographics, comorbidities, and key clinical markers. Anthropometric and body composition variables, clinical features, and inflammatory/coagulation markers were measured while fecal 16S rRNA sequencing was examined at the genus Bifidobacterium. Regression models were fitted to evaluate the relationship between gut microbiota, inflammatory/coagulation markers, and body weight in these types of diseases.

Results

The study revealed worse clinical outcomes in anthropometric, body composition, and clinical markers in low-grade MI conditions as compared to SLE. However, inflammatory and coagulation markers such as C-reactive protein (CRP) and fibrinogen were significantly more elevated in patients with SLE, which was exacerbated by high BMI/ body fat as compared to the other screened groups. An interaction analysis revealed that fibrinogen levels showed different trends when Bifidobacterium was increased depending on BMI/adiposity, which evidenced an effect modification by this microorganism in patients with SLE.

Discussion

These findings underline that gut microbiota composition, particularly the presence of Bifidobacterium, may play a crucial role in modulating inflammation and coagulation processes in patients with SLE and high fat. These insights highlight the potential of targeting gut microbiota as a therapeutic strategy to mitigate inflammation and improve clinical outcomes in SLE patients.
Unveiling mycoviral diversity in Ophiocordyceps sinensis through transcriptome analyses
Authors: Kang Qin , Zhang Jihong , Chen Fangzhou , Dong Caihong , Qin Qilian , Li Xuan , Wang Hongtuo , Zhang Huan , Meng Qian
Published: November 2024

Ophiocordyceps sinensis, an entomopathogenic fungus, infects larvae from the Lepidoptera: Hepialidae family, forming the valuable Chinese cordyceps. Mycoviruses are widespread across major lineages of filamentous fungi, oomycetes, and yeasts and have the potential to influence fungal biology and ecology. This study aimed to detect mycovirus within O. sinensis by isolating double-stranded RNA from six stains for transcriptomic sequencing and analyzing publicly available transcriptome data from 13 O. sinensis representative samples. Our analysis revealed 13 mycoviruses, with nine reported for the first time in O. sinensis. These mycoviruses are distributed across five families—Partitiviridae, Mitoviridae, Narnaviridae, Botourmiaviridae, Deltaflexiviridae—and two unclassified lineages, Ormycovirus and Vivivirus. This study also revealed frequent coinfections within individual O. sinensis strains and dynamic shifts in viral composition during fungal development. These findings enhance our knowledge of mycovirus diversity within O. sinensis and provide new insights into their taxonomy.
Associations of motor and neuropsychiatric symptoms with comorbidities in prodromal Parkinson’s disease
Authors: Chen Jia-Ru , Sun Yan , Ma Yu-Ju , Tan Lan
Published: November 2024

Objective

To investigate the associations between comorbidities and multimorbidity patterns with motor and neuropsychiatric symptoms in patients with Parkinson’s disease (PD) in prodromal PD.

Methods

Multimorbidity is defined as the coexistence of two or more long-term conditions (LTCs) (also known as multiple comorbidities). A total of 921 participants without PD were included in the Parkinson’s Progression Markers Initiative (PPMI) database and were categorized according to the LTC count. Participants were evaluated on motor and psychiatric symptoms. Pearson correlation to examine relationship of comorbidities and target symptoms. The baseline population was analyzed using Multiple linear regression model, while mixed effects model was utilized for longitudinal analysis. Fuzzy C-means clustering analysis was conducted to identify comorbidity patterns, followed by multiple linear regression for further analysis.

Results

At baseline, a higher LTC count was significantly correlated with more severe motor (MDS-UPDRS I, II, ADL, all P < 0.05) and neuropsychiatric symptoms (QUIP, P < 0.001). Three multimorbidity patterns were identified. Among them, the cardiometabolic multimorbidity pattern (CAR) had the most significant correlation with the aforementioned symptoms. Our longitudinal analysis indicated that an increase in the LTC count was associated with the exacerbation of motor and neuropsychiatric symptoms.

Conclusion

Comorbidities were cross-sectionally and longitudinally associated with the motor and neuropsychiatric symptoms of patients with prodromal PD. Among the three multimorbidity patterns, CAR posed the highest threat to the risk of more severe motor and neuropsychiatric symptoms.
Restoration of natural somatic sensations to the amputees: finding the right combination of neurostimulation methods
Authors: Soghoyan Gurgen , Biktimirov Artur R. , Piliugin Nikita S. , Matvienko Yury , Kaplan Alexander Y. , Sintsov Mikhail Y. , Lebedev Mikhail A.
Published: November 2024

Limb amputation results in such devastating consequences as loss of motor and sensory functions and phantom limb pain (PLP). Neurostimulation-based approaches have been developed to treat this condition, which provide artificial somatosensory feedback such as peripheral nerve stimulation (PNS), spinal cord stimulation (SCS), and transcutaneous electrical nerve stimulation (TENS). Yet, the effectiveness of different neurostimulation methods has been rarely tested in the same participants. Meanwhile, such tests would help to select the most effective method or a combination of methods and could contribute to the development of multisensory limb prostheses. In this study, two transhumeral amputees were implanted with stimulating electrodes placed in the medial nerve and over the spinal cord epidurally. PNS and SCS were tested in each participant as approaches to enable tactile and proprioceptive sensations and suppress PLP. Both PNS and SCS induced sensation in different parts of the phantom hand, which correlated with cortical responses detected with electroencephalographic (EEG) recordings. The sensations produced by PNS more often felt natural compared to those produced by SCS. Еvoked response potentials (ERPs) were more lateralized and adapted faster for PNS compared to SCS. In the tasks performed with the bionic hand, neurostimulation-induced sensations enabled discrimination of object size. As the participants practiced with neurostimulation, they improved on the object-size discrimination task and their sensations became more natural. А combination of PNS and TENS enabled sensations that utilized both tactile and proprioceptive information. This combination was effective to convey the perception of object softness. In addition to enabling sensations, neurostimulation led to a decrease in PLP.

Clinical trial registration

https://clinicaltrials.gov/, identifier, #NCT05650931.
Dexmedetomidine regulates the anti-oxidation and autophagy of adipose-derived stromal cells under H2O2-induced oxidative stress through Nrf2/p62 pathway and improves the retention rate of autologous fat transplantation
Authors: Li Zihao , Wei Qing , Li Yijun , Yang Fangfang , Ke Chen , Li Tian , Li Liqun , Cai Zhongming
Published: November 2024

To investigate the protective mechanism of dexmedetomidine (DEX) on adipose-derived stromal cells (ADSCs) under oxidative stress model and its promotion effect on the retention rate of adipose granule transplantation by in vitro and in vivo experiments. The experiment was divided into control group, model group (ADSCs + H2O2+normal serum), DEX group (ADSCs + H202+DEX drug-containing serum), autophagy agonist group (ADSCs + H2O2+rapamycin (RAP)+normal serum), RAP + DEX group (ADSCs + H2O2+normal serum), RAP + DEX drug-containing serum), autophagy inhibitor group (ADSCs + H2O2+chloroquine (CQ)+normal serum), CQ + DEX group (ADSCs + H2O2+CQ + DEX drug-containing serum). HO-1, GSH-PX, SOD and CAT in ADSCs under oxidative stress model were measured. ROS fluorescence intensity and apoptosis ratio were detected. Expression of Nrf2, LC3-II/LC3-I and p62 were detected. In vivo, fat mixed with ADSCs or DEX -pretreated ADSCs was implanted subcutaneously in the lower back region of nude mice. Fat grafts were collected and analyzed at 2-, 4-, 6-, and 8-weeks post-transplantation. DEX pretreatment could reduce the expression of p62 to enhance the autophagy level of ADSCs under oxidative stress model. Additionally, cotransplantation of DEX-pretreated ADSCs with fat improved the long-term texture of fat grafts. DEX increased the fat graft survival and angiogenesis.
Semen Cuscutae flavonoids activated the cAMP-PKA-CREB-BDNF pathway and exerted an antidepressant effect in mice
Authors: Shao Qianfeng , Li Yue , Jin Lin , Zhou Sheng , Fu Xiaowei , Liu Tong , Luo Guangbin , Du Shaohui , Chen Che
Published: November 2024

Background

Semen Cuscutae flavonoids (SCFs) constitute a class of metabolites of Semen Cuscutae, a botanical drug that was recently found to have an anti-depression effect. This study aimed to evaluate the anti-depression effects of SCFs in chronic unpredictable mild stress (CUMS)-induced mice and to interrogate the underlying mechanisms.

Materials and methods

The CUMS mice were used for assessing the effects of SCFs treatments on depression. Mice were randomly divided into five groups. Four groups were subjected to the CUMS induction and concomitantly administered orally with either the vehicle or with a high-, medium-, and low-dose of SCFs, once per day for 4 weeks. One group was kept untreated as a control. The mice were then assessed for their statuses of a number of depression-related parameters, including body weight, food intake, sucrose preference test (SPT), open field test (OFT), tail suspension test (TST), and forced swim test (FST). In addition, a day after the completion of these tests, biopsies from the hippocampus were harvested and used to perform metabolomics by HPLC-MS/MS and to assess the levels of cAMP by ELISA and the levels of PKA, CREB, p-CREB, and BDNF by Western blot analyses.

Results

SCFs resulted in significant increases in both body weight and food intake and in the amelioration of the depressive-like behaviors in CUMS mice. A high-dose SCFs treatment led to significant alterations in 72 metabolites, of which 26 were identified as potential biomarkers for the SCFs treatment. These metabolites are associated with lipid, amino acid, and nucleotide metabolism. Among 26 metabolites, cAMP was positively correlated with body weight, SPT, OFT-total distance, and OFT-central residence time, while negatively correlated with immobility time in TST and FST, linking a change in cAMP with the SCFs treatment and the significant improvement in depressive symptoms in CUMS mice. Further analyses revealed that the levels of cAMP, PKA, CREB, p-CREB, and BDNF were reduced in the hippocampus of CUMS mice but were all increased following the SCFs treatments.

Conclusion

SCFs could ameliorate hippocampal metabolic disturbances and depressive behaviors and cause the activation of the cAMP-PKA-CREB-BDNF signaling pathway in the hippocampus of CUMS mice.
Comparing commercial pharmacogenetic testing results and recommendations for antidepressants with established CPIC guidelines
Authors: Nguyen Tiffany T. , Leary Emili J. W. , Lee Joshua T. , Shukla Sanjay K. , Griesbach Sara A.
Published: November 2024

Introduction

Increasingly, pharmacogenetic testing helps providers with medication selection based upon patient-specific DNA results. While several government-funded organizations work towards consensus and standardization for testing and interpretation, compliance to these best practices remains inconsistent. Pharmacogenetic testing companies often develop proprietary practices for interpreting and reporting, which can lead to incongruency of reported results among companies and potential discrepancies in interpretation.

Methods

To identify the differences of commercial pharmacogenetic testing vendors’ interpretation of genotype-to-phenotype translations and medication recommendations from the Clinical Pharmacogenetic Implementation Consortium (CPIC) guidelines, a retrospective manual chart review was completed in a large rural healthcare system that utilizes two institution-approved pharmacogenetic vendors. One hundred patients were evaluated: 50 who completed testing through Company A and 50 who completed testing through Company B. Genes of interest for genotype-to-phenotype translation included CYP2B6, CYP2C19, and CYP2D6. Comparison of medication recommendations for drug-gene pairs sertraline (CYP2B6 and/or CYP2C19), escitalopram (CYP2C19), and paroxetine (CYP2D6) were compared with recommendations from CPIC, with consideration of the CPIC Serotonin Reuptake Inhibitor Antidepressants (SSRI) guideline 2023 update. This was accomplished via a novel binning process to enable comparison of company-provided binned medication recommendations with CPIC guideline recommendations. Briefly, the binning system included three categorizations based upon the relevant CPIC guideline recommendations–no action needed (green), recommend monitoring (yellow) and therapeutic intervention or alternative recommended (red).

Results

There were 32/250 (12.8%) genotype-to-phenotype translation discrepancies from CPIC guidelines, all from Company A. Of 266 evaluated binned medication recommendations, there were 114 (42.9%) discrepancies between the pharmacogenetic testing companies (Company A: 93 discrepancies, Company B: 21 discrepancies) and CPIC’s guideline based upon comparison with the novel binning system.

Discussion

Significant differences were observed between testing companies’ interpretations and recommendations, which is concerning as these discrepancies could lead to providers making medication decisions that are not supported by CPIC’s clinical practice guidelines. This may result in suboptimal outcomes for patients, leading to patient and provider dissatisfaction and erosion of trust with pharmacogenetic testing. A proposed resolution for the discrepancies in company-to-company interpretation is adherence to the CPIC guidelines and transparency in interpretation practices.
Assessing real-world safety of plecanatide: a pharmacovigilance study based on the FDA adverse event reporting system
Authors: Zhang Zhiyuan , Yao Yifan , Zhu Li
Published: November 2024

Background

Plecanatide is a selective gastrointestinal peptide used for the treatment of chronic idiopathic constipation (CIC) and irritable bowel syndrome with constipation (IBS-C). Given its widespread use, understanding the long-term safety of plecanatide in real-world settings is essential.

Methods

Data for this study were sourced from the United States Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) from the first quarter of 2017 to the second quarter of 2024. Disproportionality analysis methods were employed to quantify adverse event signals associated with plecanatide. Additionally, a Weibull distribution analysis was conducted to assess changes in adverse events over time. Sensitivity analyses were performed to enhance the robustness of the findings.

Results

A total of 861 cases associated with plecanatide were identified, including 2057 adverse event reports. Common positive adverse events included diarrhea, constipation, abdominal distension, dissatisfaction with treatment, rectal tenesmus, increased fecal volume, abnormal gastrointestinal sounds, and gastrointestinal motility disorders. The majority of adverse events related to plecanatide occurred within the first 7 days of treatment. Findings were consistent across sensitivity analyses.

Conclusion

This study preliminarily explores the safety of plecanatide in real-world applications, revealing significant new adverse event signals. These findings provide important safety references for clinicians prescribing plecanatide for CIC and IBS-C.
Signal pathways involved in contrast-induced acute kidney injury
Authors: Deng Ke , Pei Mingxin , Li Beibei , Yang Nanqi , Wang Zijian , Wan Xinchi , Zhong Zhiying , Yang Zhiyi , Chen Yanling
Published: November 2024

Contrast-induced acute kidney injury (CI-AKI) has emerged as a global public health concern, ranking as the third most prevalent cause of hospital-acquired acute kidney injury, which is related to adverse outcomes. However, its precise pathogenesis remains elusive. Consequently, researchers are dedicated to uncovering CI-AKI’s pathophysiology and signaling pathways, including inflammation, oxidative stress, apoptosis, and ferroptosis, to improve prevention and treatment. This review thoroughly analyzes the signaling pathways and their interactions associated with CI-AKI, assesses the impact of various research models on pathway analysis, and explores more precise targeted treatment and prevention approaches. Aims to furnish a robust theoretical foundation for the molecular mechanisms underpinning clinical treatments.
The impact of flywheel resistance squat training on lower limb strength in female college basketball players
Authors: Xie Lin , Qu Wenhao , Dai Jing , Xu Jiamin , Zhang Wenfeng , Sun Jian , Song Wenfeng , Li Duanying
Published: November 2024

Objective

This study compared the effects of Flywheel Resistance Squat Training (FRST) vs. Traditional Resistance Squat Training (TRST) on lower body strength in female collegiate basketball players.

Methods

Nineteen participants were randomly assigned to either the FRST group (n = 9) or the TRST group (n = 10) through a random number draw. Both groups underwent a 6-week intervention with training sessions conducted twice a week. The FRST group utilized an inertia of 0.075 kg ·m2, while another group trained at 80% of their one-repetition maximum (1RM). Each training session consisted of 4 sets of 8 repetitions with a 3-minute rest between sets. Both groups performed standardized warm-ups and stretches before and after each training session. The effectiveness of the training methods was assessed through the Countermovement Jump (CMJ), Reactive Strength Index (RSI), Eccentric Utilization Ratio, Running Vertical Jump, and 1RM squat.

Results

The FRST and TRST groups showed differences within groups in both CMJ and 1RM squat (p < 0.01), with the FRST group demonstrating moderate effect sizes in CMJ (Hedges’ g = 0.59) and 1RM (Hedges’ g = 1.01). However, there were no differences between groups (p > 0.05). The Eccentric Utilization Ratio showed a small effect size (p = 0.78; ηp2=0.01). Additionally, Reactive Strength Index and Running Vertical Jump exhibited low test-retest reliability.

Conclusion

The two groups did not exhibit a statistically significant difference. Nonetheless, both FRST and TRST demonstrated positive effects on 1RM squat and CMJ performance compared to baseline values for each method. Therefore, flywheel resistance training can be considered an effective alternative to traditional resistance training for enhancing strength levels in female basketball players.
Genome-wide identification and analysis of expression patterns of the ABC1K gene family members in Medicago sativa
Authors: Chen Zhengqiang , Chen Fangqi , Qin Yaxuan , Wang Le , Jia Ruifang , Zhao Jun , Lin Kejian , Zhang Yuanyuan
Published: November 2024

The ABC1K (activity of bc1 complex kinase) atypical protein kinase family regulates diverse physiological functions in plants, including the development, growth, and response of plants to various stress stimuli. However, to date, only a few members of the alfalfa (Medicago sativa) ABC1K gene family have been identified, which severely limits the exploration of the functional mechanism of alfalfa ABC1K. Here, we identified 22 ABC1K genes from the alfalfa genome and categorized them into four types on the basis of phylogenetic analysis results and gene structure. We then characterized the physical and biochemical properties, chromosomal location, subcellular localization, cis-regulatory elements, and conserved motifs of these genes. Transcript profiling analysis confirmed that MsABC1Ks were widely expressed in various alfalfa tissues, with tissue-specific expression. We also found that salt and drought conditions significantly regulated MsABC1K gene expression, thus indicating that MsABC1K genes perform critical functions in alfalfa’s response to abiotic stress. In summary, the findings of our study serve as an important basis to enhance the stress resistance of alfalfa and provide valuable insights to better comprehend the functions of the MsABC1K gene family.
Development of a 50K SNP array for whole-genome analysis and its application in the genetic localization of eggplant (Solanum melongena L.) fruit shape
Authors: Yu Chuying , Yang Qihong , Li Weiliu , Jiang Yaqin , Gan Guiyun , Cai Liangyu , Li Xinchun , Li Zhiqiang , Li Wenjia , Zou Min , Yang Yang , Wang Yikui
Published: November 2024

Introduction

Current eggplant variety breeding is still mainly based on conventional methods, and there remains a lack of effective molecular breeding systems for complex traits controlled by multiple genes, such as yield and quality. To accelerate the research progress of eggplant genetics and molecular breeding, it is necessary to implement a genome-based breeding strategy.

Methods

Therefore, in this study, a SNP array containing 50K liquid-phase probes was designed on the basis of the resequencing data of 577 eggplants.

Results

The developed 50K liquid-phase probes were used to perform targeted capture sequencing on 12 eggplant lines, and the efficiency of probe capture exceeded 99.25%. Principal component, phylogenetic, and population structure analyses divided the 577 eggplants into 7 subgroups, and statistical analysis was performed on the fruit shape and color of the materials in the different subgroups. Further analysis of the geographical distribution of 428 Chinese eggplant materials revealed that the geographical regions of different subgroups were similar. The 50K SNP liquid-phase array was used to perform bulked- segregant analysis combined with whole-genome resequencing (BSA-seq) of fruit shape in the F2 population, which consisted of 1435 lines constructed with E421 as the maternal parent and 145 as the paternal parent. The BSA-seq data were located in the 78444173−84449348 interval on chromosome 3, with a size of 6 Mb, which was narrowed to 712.6 kb through fine mapping. Further sequence alignment and expression analysis revealed SmIQD14 as a candidate gene controlling eggplant fruit shape. The 50K SNP liquid-phase array can be widely used in future eggplant molecular breeding research.
The neural basis underlying the association between parents’ socioeconomic status and depressive symptoms among college students
Authors: Xiao Yao , Jiang Xinting , Li Yuan , Mao Yu , Zhou Duyi
Published: November 2024

Objective

Depression is increasingly prevalent among adolescents, with parents’ socioeconomic status (SES) serving as significant predictors. Understanding the link between parents’ SES and college students’ depressive symptoms is of paramount concern. However, the neural basis linking the association between parents’ SES and students’ depressive symptoms still remains to be explored. In order to address this issue, this study aims to investigate the relationship between parents’ SES and students’ depressive symptoms, and the role of brain functional connectivity (FC) pattern in this relationship.

Methods

In this study, a total of 363 college students without a history of mental or neurological disorders underwent depressive symptoms assessment and resting-state functional magnetic resonance imaging scans. We used a connectome-based predictive modeling (CPM) approach to identify neural biomarkers of depressive symptoms.

Results

The results indicate that there is a negative correlation between parents’ SES and students’ depression tendencies (Father’s education level and SDS: r  = −0.119, p < 0.05; Mother’s education level and SDS: r  = −0.117, p < 0.05), suggesting that students whose parents have a higher educational level are less likely to suffer from depression. Furthermore, a FC pattern that can significantly predict depressive symptoms outside of the body was identified (r  = 0.13, p < 0.005), with most of the FCs belonging to the default mode network (DMN) and ventral attention network (VAN). Additionally, the FC pattern associated with depressive symptoms mediate the relationship between parents’ SES and depressive symptoms.

Conclusion

Therefore, we believe that improving the education levels of parents may have a practical effect in reducing depressive symptoms among adolescents.
Association of the neutrophil-to-platelet ratio with response to electroconvulsive therapy in adolescents with major depressive disorder
Authors: Geng Dandan , Wang Wenxin , Du Ning , Niwenahisemo Lisa Cynthia , Xu Heyan , Wang Yuna , Kuang Li
Published: November 2024

Background

Major depressive disorder (MDD) is one of the most serious mental disorders affecting adolescents worldwide. Electroconvulsive therapy (ECT) is widely acknowledged as a first-line treatment for severe depression, but the clinical response varies. Neutrophils and platelets are both related to the progression of MDD. The aim of this study was to investigate the correlation between the neutrophil-to-platelet ratio (NPR) during the acute phase and the effectiveness of ECT treatment.

Methods

A total of 138 adolescent MDD patients who received ECT were included in the study. Neutrophil and platelet levels were obtained upon admission. At the same time, treatment response was the primary outcome measure, defined as a reduction of ≥ 50% in the HAMD-17 score from baseline to treatment endpoint, and the secondary outcome measure was remission of depression, defined as a HAMD-17 score ≤ 7.

Results

After receiving ECT, 103(74.6%) of all patients responded to treatment and 72(52.2%) achieved remission. Non-responders/non-remitters to ECT tended to have higher levels of NPR at baseline compared to ECT responders/remitters [Non-responder: 3.4 (2.5-4.8) vs 2.7 (2.2-3.5), P = 0.002; Non-remitter: 0.014 (0.011-0.017) vs 0.011 (0.008-0.015), P = 0.03]. In multiple logistic regression, high NPR (≥ 0.014) remained independently associated with ECT non-response/non-remission after adjusting for confounding factors [Non-responder: OR = 4.911, 95% CI (2.052 - 11.754), P < 0.001; Non-remitter: OR = 2.704, 95% CI (1.262 - 5.796), P = 0.011].

Conclusion

High NPR correlates with poor ECT efficacy in adolescents with MDD, particularly among female and overweight patients.
A bibliometric analysis of mental health among high school students
Authors: Song Shilong , Yu Wenbing , Li Shuoqi , Sun Wenze , Fu Jiannan , Cheng Qi
Published: November 2024

Background

In recent years, with the increase in academic pressure and changes in social environment, especially after the outbreak of COVID-19, there has been a significant impact on the mental health of high school students. This trend, which is concerning, requires a systematic bibliometric analysis to develop effective preventive and remedial measures.

Objective

This study aims to identify and analysis the research hotspots, frontiers and emerging trends in the field of high school students’ mental health over the past two decades using CiteSpace software. These findings provide important insights that can shape future research agendas and guide targeted interventions to improve the mental health outcomes of this vulnerable group.

Methods

This study utilized the Web of Science Core Collection database as its data source to retrieve literature pertaining to high school students’ mental health from January 1, 2004, to January 1, 2023. The initial search yielded 1,764 relevant documents. After manually screening to exclude duplicates, conference proceedings, announcements, and irrelevant documents, a total of 1,748 relevant documents were retained. The research employed the CiteSpace 6.2.R4 tool to evaluate various bibliometric indicators of the included literature, including statistics on institutional and author publication volumes, as well as co-citation analysis.

Results

A total of 1,748 English-language documents were retrieved, showing an upward trend in publications on high school students’ mental health from 2004 to 2024. The research hotspots primarily focus on COVID-19 pandemic, depression, bullying, substance abuse, sexual behavior, Physical Education and their relationships with the mental health of high school students. Future research trends may focus on studies investigating the relationships between the use of electronic cigarettes, sleep disorders, internet addiction, and the mental health of high school students.

Conclusion

In addition to the six major research hotspots of COVID-19 pandemic, Depression and stress, Suicidal ideation, Bullying, Sexual behavior and Physical education. Researches should pay more attention to the use of electronic cigarettes, sleep disorders, and Internet addiction among high school students.
Dysregulation of dopamine neurotransmission in drug addicts: implications for criminal behavior and corrective interventions
Authors: Gu Chao , Geng Yu-chang , Zhu Li-na
Published: November 2024

Drug addiction often correlates with criminal behavior. When investigating criminal behavior among individuals grappling with drug addiction, it becomes crucial to scrutinize the influence of dopamine. Substances such as heroin, morphine, methamphetamine and other drugs can cause abnormal dopamine secretion when people are addicted to them, which promotes changes in the brain’s reward circuit and emotional balance, thereby increasing susceptibility to criminal behavior. The pivotal role of dopamine within the reward pathway and its regulatory function in emotional processes exert profound influence on behavior following drug simulation. These influences are primarily manifested by three distinct attributes: a singular criminal motive and objective, lack of moral sense, and impulsive decision-making processes. Drawing upon the distinctive dopaminergic dynamics inherent in individuals afflicted by drug addiction, this study advocates for targeted corrective interventions. The preventive paradigm encompasses the cultivation of supportive community environments, the establishment of comprehensive databases, and providing legal education and protection, among other initiatives. In terms of treatment, along with judicial sanctions and protections, exercise regimens and psychotherapeutic interventions are advocated. The corrective endeavor necessitates a synergistic integration of community-based and legalistic frameworks. The objective is to furnish guiding principles for tackling criminal behavior precipitated by aberrant dopamine secretion, underpinned by a scientifically informed approach.
Between compulsions and contagions: examining the protective role of OCD against COVID-19 in a large cohort study
Authors: Avni Chen , Sinai Dana , Toren Paz
Published: November 2024

Introduction

Since the onset of the COVID-19 pandemic in 2020, a significant body of research has explored the impact of the virus and its preventative measures on mental health among individuals with OCD. However, to our knowledge, no study has been conducted to test whether the very behaviors considered symptomatic of OCD inadvertently offer a protective shield against COVID-19 infection.

Methods

This retrospective cohort study utilized the electronic health record database of Israel’s largest healthcare provider, Clalit Health Services (CHS), to compare patients with and without recorded OCD diagnoses in terms of the number of COVID-19 tests taken, hospitalizations, vaccination rates, and infection rates during and after different pandemic waves.

Results

The OCD group had a slightly higher rate of positive COVID-19 tests compared to the control group (p<0.001), but only since the easing of restrictions after the end of the fifth wave. The OCD group was also more likely to receive a third dose of the COVID-19 vaccine (p<0.001).

Discussion

Our findings suggest that OCD may not confer protection against COVID-19 and may even be associated with slightly higher infection rates, particularly in the post-restrictions period.