bluuebunny/crossref_metadata_2025 huggingface dataset:
Toward an Integrated Model of Supportive Peer Relationships in Early Adolescence: A Systematic Review and Exploratory Meta-Analysis
Authors: Mitic Marija , Woodcock Kate A. , Amering Michaela , Krammer Ina , Stiehl Katharina A. M. , Zehetmayer Sonja , Schrank Beate
Published: February 2021

Supportive peer relationships (SPR) are crucial for mental and physical health. Early adolescence is an especially important period in which peer influence and school environment strongly shape psychological development and maturation of core social-emotional regulatory functions. Yet, there is no integrated evidence based model of SPR in this age group to inform future research and practice. The current meta-analysis synthetizes evidence from 364 studies into an integrated model of potential determinants of SPR in early adolescence. The model encompasses links with 93 variables referring to individual (identity, skills/strengths, affect/well-being, and behavior/health) and environmental (peer group, school, family, community, and internet/technology) potential influences on SPR based on cross-sectional correlational data. Findings suggest the central importance of identity and social–emotional skills in SPR. School environment stands out as a compelling setting for future prevention programs. Finally, we underscore an alarming gap of research on the influence of the virtual and online environment on youth's social realm given its unquestionable importance as a globally expanding social interaction setting. Hence, we propose an integrated model that can serve as organizational framework, which may ultimately lead to the adoption of a more structured and integrated approach to understanding peer relationship processes in youth and contribute to overcoming marked fragmentation in the field.
Cognitive Predictors of Precautionary Behavior During the COVID-19 Pandemic
Authors: Thoma Volker , Weiss-Cohen Leonardo , Filkuková Petra , Ayton Peter
Published: February 2021

The attempts to mitigate the unprecedented health, economic, and social disruptions caused by the COVID-19 pandemic are largely dependent on establishing compliance to behavioral guidelines and rules that reduce the risk of infection. Here, by conducting an online survey that tested participants’ knowledge about the disease and measured demographic, attitudinal, and cognitive variables, we identify predictors of self-reported social distancing and hygiene behavior. To investigate the cognitive processes underlying health-prevention behavior in the pandemic, we co-opted the dual-process model of thinking to measure participants’ propensities for automatic and intuitive thinking vs. controlled and reflective thinking. Self-reports of 17 precautionary behaviors, including regular hand washing, social distancing, and wearing a face mask, served as a dependent measure. The results of hierarchical regressions showed that age, risk-taking propensity, and concern about the pandemic predicted adoption of precautionary behavior. Variance in cognitive processes also predicted precautionary behavior: participants with higher scores for controlled thinking (measured with the Cognitive Reflection Test) reported less adherence to specific guidelines, as did respondents with a poor understanding of the infection and transmission mechanism of the COVID-19 virus. The predictive power of this model was comparable to an approach (Theory of Planned Behavior) based on attitudes to health behavior. Given these results, we propose the inclusion of measures of cognitive reflection and mental model variables in predictive models of compliance, and future studies of precautionary behavior to establish how cognitive variables are linked with people’s information processing and social norms.
The Varying Coherences of Implied Motion Modulates the Subjective Time Perception
Authors: Li Feiming , Wang Lei , Jia Lei , Lu Jiahao , Wu Youping , Wang Cheng , Wang Jun
Published: February 2021

Previous research has demonstrated that duration of implied motion (IM) was dilated, whereas hMT+ activity related to perceptual processes on IM stimuli could be modulated by their motion coherence. Based on these findings, the present study aimed to examine whether subjective time perception of IM stimuli would be influenced by varying coherence levels. A temporal bisection task was used to measure the subjective experience of time, in which photographic stimuli showing a human moving in four directions (left, right, toward, or away from the viewer) were presented as probe stimuli. The varying coherence of these IM stimuli was manipulated by changing the percentage of pictures implying movement in one direction. Participants were required to judge whether the duration of probe stimulus was more similar to the long or short pre-presented standard duration. As predicted, the point of subjective equality was significantly modulated by the varying coherence of the IM stimuli, but not for no-IM stimuli. This finding suggests that coherence level might be a key mediating factor for perceived duration of IM images, and top-down perceptual stream from inferred motion could influence subjective experience of time perception.
Interpretation of Quantities Displayed in Pictorial Charts
Authors: Rolfes Tobias
Published: February 2021

This brief research report presents an experiment investigating how people interpret quantities displayed in pictorial charts. Pictorial charts are a popular form of data visualization in media. They represent different quantities with differently scaled pictures. In the present study, 63 university students answered a 12-item questionnaire containing three different pictorial charts. The study aimed to evaluate how individuals perceive the quantities in the pictorial charts intuitively. Therefore, the students’ answers were not rated as correct or incorrect. Instead, it was analyzed which functional relationship between scale factor and estimated quantity best described people’s interpretation of pictorial charts. The experiment showed that, on average, a model assuming a quadratic relationship fitted best. This result deviates from research that found an overgeneralization of linearity when students compare the areas of two mathematically similar shapes. It may be that the routines for the interpretation of pictures differ considerably depending on whether a person must calculate a quantity arithmetically or is prompted to estimate the quantity based on visual perception.
Studying Spatial Visual Attention: The Attention-Window Task as a Measurement Tool for the Shape and Maximum Spread of the Attention Window
Authors: Klatt Stefanie , Memmert Daniel
Published: February 2021

Visual attentional processes have been an important topic in psychological research for years. Over the last few decades, new methods have been developed, aiming to explore the characteristics of the focus of attention in more detail. Studies that applied the “Attention-Window Task” (AWT) quantified the maximum extent of the “Attention Window” (AW) along its horizontal, vertical, and diagonal meridians, when subjects were required to perceive two peripheral stimuli simultaneously. In three experiments using the AWT, we investigated the effects of cue validity (Experiment 1), stimulus-onset asynchrony (SOA) (i.e., the interval between the onset of the cues and the onset of the target stimuli), and target stimuli complexity (Experiment 3) on the size and shape of the AW. Results showed that the AW was greater under valid cue conditions compared to invalid conditions, when the locations of cue and target stimuli differed. Furthermore, the AW decreased when the SOA between the cue and targets was reduced and also when the task complexity was higher and more objects within the target stimuli had to be classified. Overall, it can be stated that the AWT with its possible task changes and adjustments can be considered as a potential standard tool to measure the maximum spread and shape of the spatial AW.
Cross-Modal Transfer Following Auditory Task-Switching Training in Old Adults
Authors: Toovey Benjamin Robert William , Kattner Florian , Schubert Torsten
Published: February 2021

Maintaining and coordinating multiple task-sets is difficult and leads to costs, however task-switching training can reduce these deficits. A recent study in young adults demonstrated that this training effect occurs at an amodal processing level. Old age is associated with reduced cognitive plasticity and further increases the performance costs when mixing multiple tasks. Thus, cognitive aging might be a limiting factor for inducing cross-modal training effects in a task-switching environment. We trained participants, aged 62–83 years, with an auditory task-switching paradigm over four sessions (2880 total trials), to investigate whether training-related reductions in task-switching costs would also manifest in an untrained visual modality version of the task. Two control groups trained with single tasks (active control) or not trained (passive control) allowed us to identify improvements specific to task-switching training. To make statistical evaluations of any age differences in training and cross-modal transfer, the data from the Kattner cohort were incorporated into the present analysis. Despite the tendency for older adults to respond more cautiously, task-switching training specifically led to a mixing cost reduction in both trained and untrained modalities, the magnitude of which was statistically similar regardless of age. In line with a growing body of research, we failed to observe any far transfer effects in measures of inhibition, working memory or fluid intelligence. Overall, we conclude that any apparent cognitive limitations associated with aging do not prevent cognitive control processes which support set-shifting from improving at an amodal level.
The Influence of Chinese Machiavellianism and Moral Identity on the Level of Anxiety in Moral Dilemma Situations in Chinese Students
Authors: Tang Shujun , Li Kai
Published: February 2021

Based on the conflict-of-values theory, this study examines the influences of Machiavellianism and ethical values on anxiety in college students when they face moral dilemmas. Questionnaires on the Chinese equivalent of Machiavellianism, moral identity, and anxiety were completed by 115 Chinese college students. The results suggest that Machiavellianism and ethical values influence anxiety, and the interaction between ethical values and Machiavellianism is significant—among individuals with high ethical values, those with high levels of Machiavellianism exhibit markedly higher levels of anxiety than those with low levels of Machiavellianism. However, among individuals with low ethical values, there is no difference in anxiety between those with low or high levels of Machiavellianism. This research, depicting complex relationships among anxiety, Machiavellianism, and ethical values, suggests that leading people to do good deeds not only requires high ethical values but also necessitates the resistance to negative values such as Machiavellianism.
University Social Responsibility, Service Learning, and Students' Personal, Professional, and Civic Education
Authors: Coelho Márcia , Menezes Isabel
Published: February 2021

The long-standing vision of universities as the “alma mater” of students and graduates is a demonstration of its role as sustaining the person, the expert/professional, and the citizen. This role has persisted in the face of rising global challenges such as the emergence of new learning spaces, the growing diversity of publics, the call for productivity and performativity, and the hope for a significant engagement with the community and the public good. These sometimes conflicting tendencies have also stimulated higher education institutions to further pedagogical strategies that articulate in novel ways the classical elements of learning: action/experience and reflection/theory. In this context, service learning received a new impetus, particularly in the post-Bologna European Higher Education Area, as universities were looking for ways in which to articulate the social dimension of HEI and their “third mission” as institutions not only committed to addressing and solving societal problems, but also committed to fostering public-minded alumni through powerful experiences of engagement for both the students and the community.

This paper is based on the experience of Erasmus+project ESSA, a service earning based project focused on University social responsibility (USR). ESSA engaged four groups of students from three European universities (Edinburgh, Porto, and Kaunas) in conducting a 1-week on-site USR audit based on an ecologic and situated concept of social responsibility. We will consider the perceived impact of ESSA on 44 students through a thematic analysis of focus group discussions and student self-assessment reports produced during and after their participation in the USR audit.
The Bottleneck Metaphor of Leadership Culture: How Shared Understandings About Leadership Develop in Groups and Impede Diversity and Effectiveness of Leaders
Authors: Özcan Muaz
Published: February 2021

There are two big problems related to leadership today: unequal representation and high failure rates among leaders. This conceptual paper argues that commonly shared values, assumptions, and beliefs about leadership, i.e., universal leadership culture, are the common cause of both problems. After the concepts and levels related to leadership culture were explained, we introduce a multilevel, multi-actor process model named the bottleneck metaphor of leadership culture. This metaphor describes how leadership cultures are co-constructed by multiple actors based on their involvement in leader selection and reproduce themselves in groups over time based on emergent leaders' characteristics. Next, a diagnostic tool called “the leadership mirror” is proposed for organizations that want to assess their leadership culture's current state as a starting point for further interventions. Specific suggestions are made for various actors, ranging from individuals to organizations, for their possible roles in preventing undesired leadership cultures.
Dance Intervention Affects Social Connections and Body Appreciation Among Older Adults in the Long Term Despite COVID-19 Social Isolation: A Mixed Methods Pilot Study
Authors: Hansen Pil , Main Caitlin , Hartling Liza
Published: February 2021

The ability of dance to address social isolation is argued, but there is a lack of both evidence of such an effect and interventions designed for the purpose. An interdisciplinary research team at University of Calgary partnered with Kaeja d’Dance to pilot test the effects of an intervention designed to facilitate embodied social connections among older adults. Within a mixed methods study design, pre and post behavioral tests and qualitative surveys about experiences of the body and connecting were administered to thirteen participants along with test instruments of loneliness and body appreciation. In the short-term, no significant changes were found on quantitative tests. Exploratory analysis revealed intervention improvements on individual body appreciation questions only. This indication of change was strongly supported by converging qualitative data and identified as relating to: increased connection through task-based collaboration, increased awareness of interpersonal boundaries, and a shift to experiencing the body as responsive. These indications of increased relational capacity were deemed likely to cause further impact in the long term. Examining this possibility and the subsequently arisen factor of COVID-19 risks and restrictions, test instruments were administered again to 10 participants 4 and 5 months after the intervention. A significant increase in loneliness was found. Despite this negative impact of COVID-19 isolation, several positive intervention changes remained detectable and some continued to increase over time. Seventy percent of the participants, who made new social contacts during the intervention and later sought continued contact, improved significantly across all body appreciation measures over the full study. The qualitative data from the last two time-points revealed both consistent values and new, negative changes. While these preliminary findings speak to the durability of intervention changes, they also identify areas of urgent priority to help older adults restore embodied relational capacity that has declined during COVID-19. Within the limitations of a small-sample pilot study, converging mixed methods results support the hypothesis that dance interventions designed for the purpose can positively affect the social inclusion of older adults. Although we recommend further study, these promising results also indicate that dance interventions can help older adults recover from pandemic isolation.
Depressive Symptoms and Emotional Distress of Transnational Mothers: A Scoping Review
Authors: Pineros-Leano María , Yao Laura , Yousuf Aroub , Oliveira Gabrielle
Published: February 2021

Background: Female led migration is a recent trend that has been gaining momentum, particularly in Latin America. However, little attention has been given to the psychological consequences of mothers who leave their children in their country of origin and migrate to a host country to work. Therefore, it is important to investigate the mental health status of transnational mothers and to further identify issues for intervention and supportive services.

Methods: PubMed, PsycINFO, ERIC, CENTRAL, Scopus, and ScienceDirect databases were searched systematically for peer-reviewed articles published from inception through July 2019. The search included the following terms: migrant, immigrant, transnational, transnational mother, AND mood disorders, depressive symptoms, and depression. Initially, 8,375 studies were identified. After exclusionary criteria were applied, 17 studies were identified and included in the review.

Results: We found six quantitative studies that investigated depressive symptoms among transnational mothers. Of these studies, three found a positive association between transnational motherhood and depressive symptoms; three of these articles found a null correlation. A total of eight qualitative studies and three mixed-methods studies were found that addressed depressive symptoms and emotional distress among transnational mothers. The eight qualitative studies identified highlighted the significant emotional distress transnational mothers experience. Lastly, the three mixed-methods studies similarly discussed the emotional hardships faced by transnational mothers.

Implications: The studies identified suggest that depressive symptoms and emotional distress are prevalent among transnational mothers. Therefore, public health social workers and other mental health providers need to focus on developing strategies to identify and screen transnational mothers for depressive symptoms.
Evolving Concepts of the Schizophrenia Spectrum: A Research Domain Criteria Perspective
Authors: Cuthbert Bruce N. , Morris Sarah E.
Published: February 2021

Several trends intersecting over the past two decades have generated increasing debate as to how the concepts of schizophrenia, the schizophrenia spectrum, and the psychotic disorders spectrum should be regarded. These trends are reflected in various areas of research such as genomics, neuroimaging, and data-driven computational studies of multiple response systems. Growing evidence suggests that schizophrenia represents a broad and heterogenous syndrome, rather than a specific disease entity, that is part of a multi-faceted psychosis spectrum. Progress in explicating these various developments has been hampered by the dependence upon sets of symptoms and signs for determining a diagnosis, and by the reliance on traditional diagnostic categories in reviewing clinical research grants. To address these concerns, the U.S. National Institute of Mental Health initiated the Research Domain Criteria (RDoC) project, a translational research program that calls for studies designed in terms of empirically-based functions (such as cognitive control or reward learning) rather than diagnostic groups. RDoC is a research framework rather than an alternative diagnostic system, intended to provide data that can inform future nosological manuals. This commentary includes a brief summary of RDoC as it pertains to schizophrenia and psychotic spectra, examples of recent data that highlight the utility of the approach, and conclusions regarding the implications for evolving conceptualizations of serious mental illness.
The Urban Metabolism of Lima: Perspectives and Policy Indications for GHG Emission Reductions
Authors: Facchini Angelo , Mele R. , Caldarelli Guido
Published: February 2021

The urban metabolism of Metropolitan Lima, the largest urban agglomeration in Peru, which has recently surpassed 10 million inhabitants, was analyzed in this study. This coastal megacity, located within the Rimac, Chillón, and Lurin watersheds constitutes the socioeconomic center of the country and is the hub of the main import and export routes. A multi-layer approach was used to explore material and energy flows in the Peruvian capital for a 10-year timeframe. Results show that in 2006 the GDP of the 49 districts that shape the metropolitan area of Lima was 105.2 billion USD-PPP, while in 2014 it reached about 200 billion USD-PPP. Based on this growth, we highlight that energy, electricity and water flows experienced a linear increase with respect to GDP, being the electricity consumption in years 2006, 2011 and 2014, 7295, 10,112, and 11,465 GWh, respectively. Regarding demographics, population growth ratios of GDP (650%), electricity consumption (400%), solid waste production (250%), and water (100%) confirm the results of super linear scaling found by Kennedy et al. (2015) for the other megacities. Finally, the increase in greenhouse gas (GHG) emissions was computed following an important shift in the primary energy sources to produce electricity. The most important change was linked to the shift from hydropower to natural gas, a trend that initiated in 2006. For instance, in 2001 79% of the total electricity production came from hydropower, whereas in 2014 69% was linked to natural gas. This shift produced an increase of GHG emissions of more than 200% in 2014 when compared to the electricity generation mix of 2001. Following these results, we strongly encourage policies for the decarbonization of the electricity production sector, as well as for mobility infrastructures, e.g., electric public and transport sector, with a progressive shift toward electric mobility.
Physical Activity and Psychological Well-Being During the COVID-19 Lockdown: Relationships With Motivational Quality and Nature Contexts
Authors: Jenkins Matthew , Houge Mackenzie Susan , Hodge Ken , Hargreaves Elaine Anne , Calverley Jessica R. , Lee Craig
Published: February 2021

The COVID-19 pandemic is a global event that has already had substantive negative impacts on psychological well-being. This study investigated the relationship between physical activity (PA) and psychological well-being during a country-wide COVID-19 lockdown in New Zealand. Motivational quality and PA context (nature-based or non-nature-based) were included as potential mediating and moderating variables within this relationship, respectively. Participants completed an online survey assessing psychological well-being, weekly PA levels, and PA during the second and third weeks of the 7 week COVID-19 lockdown period in New Zealand. Data were analysed using Partial Least Squares Structural Equation Modelling. Results showed that PA significantly predicted psychological well-being, with no significant difference evident in psychological well-being dependent on whether PA was nature or non-nature-based. Nature-based PA was a stronger predictor of intrinsic motivation compared to non-nature-based PA, and intrinsic motivation was positively associated with psychological well-being. In contrast, non-nature-based PA was a stronger predictor of introjected regulation compared to nature-based PA, which was negatively associated with psychological well-being. Overall, these findings suggest that (1) weekly PA was associated with increased psychological well-being during the lockdown, and (2) nature-based PA may foster psychological well-being via effects on motivation. The implications for continued participation in PA will be discussed.
Early Diagnosis of Iron Deficiency in Commercial Tomato Crop Using Electrical Signals
Authors: Tran Daniel , Camps Cédric
Published: February 2021

Adequate plant nutrition is essential for commercial crop production. There are 18 nutrients that are essential for proper crop development. Each is equally important to the plant, although they are required in vastly different amounts. The absence of any one of these nutrients has the potential to decrease crop yields and quality by negatively affecting associated growth factors. Hence, early diagnosis of nutrient imbalances or deficiencies is of crucial importance for farmers. In this work, we provide compelling evidence that electrical potential variation in a commercial tomato crop contains information, which can be modeled to detect iron (Fe) deficiency before visual symptoms appear. The proposed supervised machine learning model showed accurate prediction on test data of above 75%. A model built to classify normal conditions (full nutrients) vs. strong Fe deficiency conditions (visible symptoms), enables early detection of slight Fe deprivation i.e., 6 days prior to the appearance of the earliest visual symptoms. Continuous real-time monitoring of crop electrical signals and deployment of predictive algorithms could constitute a great practical tool to help and assist farmers in iron deficiency detection.
Fluid Therapy in Dogs and Cats With Sepsis
Authors: Montealegre Federico , Lyons Bridget M.
Published: February 2021

Sepsis is currently defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Sepsis may occur secondary to infection anywhere in the body, and its pathogenesis is complex and not yet fully understood. Variations in the host immune response result in diverse clinical manifestations, which complicates clinical recognition and fluid therapy both in humans and veterinary species. Septic shock is a subset of sepsis in which particularly profound circulatory, cellular, and metabolic abnormalities are associated with a greater risk of mortality than with sepsis alone. Although septic shock is a form of distributive shock, septic patients frequently present with hypovolemic and cardiogenic shock as well, further complicating fluid therapy decisions. The goals of this review are to discuss the clinical recognition of sepsis in dogs and cats, the basic mechanisms of its pathogenesis as it affects hemodynamic function, and considerations for fluid therapy. Important pathophysiologic changes, such as cellular interaction, microvascular alterations, damage to the endothelial glycocalyx, hypoalbuminemia, and immune paralysis will be also reviewed. The advantages and disadvantages of treatment with crystalloids, natural and synthetic colloids, and blood products will be discussed. Current recommendations for evaluating fluid responsiveness and the timing of vasopressor therapy will also be considered. Where available, the veterinary literature will be used to guide recommendations.
Determinization and Minimization of Automata for Nested Words Revisited
Authors: Niehren Joachim , Sakho Momar
Published: February 2021

We consider the problem of determinizing and minimizing automata for nested words in practice. For this we compile the nested regular expressions (NREs) from the usual XPath benchmark to nested word automata (NWAs). The determinization of these NWAs, however, fails to produce reasonably small automata. In the best case, huge deterministic NWAs are produced after few hours, even for relatively small NREs of the benchmark. We propose a different approach to the determinization of automata for nested words. For this, we introduce stepwise hedge automata (SHAs) that generalize naturally on both (stepwise) tree automata and on finite word automata. We then show how to determinize SHAs, yielding reasonably small deterministic automata for the NREs from the XPath benchmark. The size of deterministic SHAs automata can be reduced further by a novel minimization algorithm for a subclass of SHAs. In order to understand why the new approach to determinization and minimization works so nicely, we investigate the relationship between NWAs and SHAs further. Clearly, deterministic SHAs can be compiled to deterministic NWAs in linear time, and conversely NWAs can be compiled to nondeterministic SHAs in polynomial time. Therefore, we can use SHAs as intermediates for determinizing NWAs, while avoiding the huge size increase with the usual determinization algorithm for NWAs. Notably, the NWAs obtained from the SHAs perform bottom-up and left-to-right computations only, but no top-down computations. This NWA behavior can be distinguished syntactically by the (weak) single-entry property, suggesting a close relationship between SHAs and single-entry NWAs. In particular, it turns out that the usual determinization algorithm for NWAs behaves well for single-entry NWAs, while it quickly explodes without the single-entry property. Furthermore, it is known that the class of deterministic multi-module single-entry NWAs enjoys unique minimization. The subclass of deterministic SHAs to which our novel minimization algorithm applies is different though, in that we do not impose multiple modules. As further optimizations for reducing the sizes of the constructed SHAs, we propose schema-based cleaning and symbolic representations based on apply-else rules that can be maintained by determinization. We implemented the optimizations and report the experimental results for the automata constructed for the XPathMark benchmark.
EXPTIME Hardness of an n by n Custodian Capture Game
Authors: Ito Fumitaka , Naito Masahiko , Katabami Naoyuki , Tsukiji Tatsuie
Published: February 2021

Custodian capture occurs when a player has placed two of his pieces on the opposite sides of an orthogonal line of the opponent’s men. Each piece moves like the rook in Chess. Different cultures played it from pre-modern times in two-player strategy board games, Ludus Latrunculorum (Kowalski’s reconstruction), Hasami shogi in Japan, Mak-yek in Thailand and Myanmar, Ming Mang in Tibet, and so on. We prove that a custodian capture game on n×n square board is EXPTIME hard if the first player to capture five or more men in total wins.
Bacillus amyloliquefaciens-9 as an Alternative Approach to Cure Diarrhea in Saanen Kids
Authors: Zhang Wenying , Xin Huijie , Jiang Nannan , Lv Zhengbing , Shu Jianhong , Shi Hengbo
Published: February 2021

Bacillus amyloliquefaciens-9 (GBacillus-9), derived from the intestinal tract of the white-spotted bamboo shark, secretes a variety of antimicrobial compounds that inhibit the growth of pathogenic bacteria. In this study, the role of GBacillus-9 in the prevention and treatment of Saanen kids with diarrhea was assessed. Six healthy kids (HL) and six kids with diarrhea (DL) were selected. All kids were fed with 0.3% (w/v) GBacillus-9 (spray power) in raw milk for two weeks. The proportion of kids with diarrhea decreased gradually as the trial progressed, and 100% DL kids were cured at day 15. GBacillus-9 increased the serum immunoglobulin (Ig) G, interleukin (IL)-4, and IL-6 concentration (p < 0.05). The amplicon sequencing analysis of the fecal bacterial community revealed that the fecal microbiota was remarkably different between the HL and the DL groups at day 0. After two weeks of feeding with GBacillus-9, no significant difference in fecal microbiota was observed between HL and DL groups at the phylum level. GBacillus-9 restored the intestinal microbial disorder associated with serum immunoglobulin and interleukin concentration. Correlation analysis showed that GBacillus-9 altered globulin and interleukin concentration and that immunoglobulin was associated with Firmicutes. Collectively, our results revealed that GBacillus-9 improved the gut health of kids by improving microbial homeostasis.
Responses of Vaginal Microbiota to Dietary Supplementation with Lysozyme and its Relationship with Rectal Microbiota and Sow Performance from Late Gestation to Early Lactation
Authors: Xu Shengyu , Dong Yanpeng , Shi Jiankai , Li Zimei , Che Lianqiang , Lin Yan , Li Jian , Feng Bin , Fang Zhengfeng , Yong Zhuo , Wang Jianping , Wu De
Published: February 2021

This study was conducted to evaluate the effects of dietary lysozyme (LZM) supplementation on the vaginal microbiota, as well as the relationship between vaginal microbiota and the fecal microbiota of rectum and the reproductive performance of the sow. A total of 60 Yorkshire × Landrace sows (3–6 of parity) were arranged from day 85 of gestation to the end of lactation in a completely randomized design with three treatments (control diet, control diet + lysozyme 150 mg/kg, control diet + lysozyme 300 mg/kg). The results showed that sows fed with lysozyme increased serum interleukin-10 (IL-10, p < 0.05) on day 7 of lactation. The vaginal microbiota varied at different taxonomic levels with LZM supplementation by 16S rRNA gene sequencing. The most representative changes included a decrease in Tenericutes, Streptococcus, Bacillus and increase in Bacteroidetes, Actinobacteria, Enterococcus, and Lactobacillus (p < 0.05). There were 777 OTUs existing in both, vaginal and fecal microbiota. The addition of LZM also decreased the abundance of Tenericutes (p < 0.05) in the vagina and feces. The changes in the microbiota were correlated in some cases positively with the performance of the sow, for example, Bacillus in feces was positively correlated with the neonatal weight (p < 0.05). These results indicate that the addition of lysozyme to the diet of sow during perinatal period promote the change of vaginal bacterial community after farrowing. The variations in vaginal microbiota are also associated with the changes in the fecal microbiology of the rectum and the reproductive performance of the sow. Therefore, it is concluded that dietary supplementation with lysozyme in sows in late gestation stage until early lactation, is beneficial to establish vaginal microbiota that seems to promote maternal health and reproductive performance.
Antinuclear Autoantibodies in Health: Autoimmunity Is Not a Synonym of Autoimmune Disease
Authors: Pashnina Irina A. , Krivolapova Irina M. , Fedotkina Tamara V. , Ryabkova Varvara A. , Chereshneva Margarita V. , Churilov Leonid P. , Chereshnev Valeriy A.
Published: February 2021

The incidence of autoimmune diseases is increasing. Antinuclear antibody (ANA) testing is a critical tool for their diagnosis. However, ANA prevalence in healthy persons has increased over the last decades, especially among young people. ANA in health occurs in low concentrations, with a prevalence up to 50% in some populations, which demands a cutoff revision. This review deals with the origin and probable physiological or compensatory function of ANA in health, according to the concept of immunological clearance, theory of autoimmune regulation of cell functions, and the concept of functional autoantibodies. Considering ANA titers ≤1:320 as a serological marker of autoimmune diseases seems inappropriate. The role of anti-DFS70/LEDGFp75 autoantibodies is highlighted as a possible anti-risk biomarker for autoimmune rheumatic disorders. ANA prevalence in health is different in various regions due to several underlying causes discussed in the review, all influencing additive combinations according to the concept of the mosaic of autoimmunity. Not only are titers, but also HEp-2 IFA) staining patterns, such as AC-2, important. Accepting autoantibodies as a kind of bioregulator, not only the upper, but also the lower borders of their normal range should be determined; not only their excess, but also a lack of them or “autoimmunodeficiency” could be the reason for disorders.
Transformation of Biowaste for Medical Applications: Incorporation of Biologically Derived Silver Nanoparticles as Antimicrobial Coating
Authors: Vigneswari Sevakumaran , Amelia Tan Suet May , Hazwan Mohamad Hazari , Mouriya Govindan Kothandaraman , Bhubalan Kesaven , Amirul Al-Ashraf Abdullah , Ramakrishna Seeram
Published: February 2021

Nanobiotechnology has undoubtedly influenced major breakthroughs in medical sciences. Application of nanosized materials has made it possible for researchers to investigate a broad spectrum of treatments for diseases with minimally invasive procedures. Silver nanoparticles (AgNPs) have been a subject of investigation for numerous applications in agriculture, water treatment, biosensors, textiles, and the food industry as well as in the medical field, mainly due to their antimicrobial properties and nanoparticle nature. In general, AgNPs are known for their superior physical, chemical, and biological properties. The properties of AgNPs differ based on their methods of synthesis and to date, the biological method has been preferred because it is rapid, nontoxic, and can produce well-defined size and morphology under optimized conditions. Nevertheless, the common issue concerning biological or biobased production is its sustainability. Researchers have employed various strategies in addressing this shortcoming, such as recently testing agricultural biowastes such as fruit peels for the synthesis of AgNPs. The use of biowastes is definitely cost-effective and eco-friendly; moreover, it has been reported that the reduction process is simple and rapid with reasonably high yield. This review aims to address the developments in using fruit- and vegetable-based biowastes for biologically producing AgNPs to be applied as antimicrobial coatings in biomedical applications.
Improving Reproductive Performance and Health of Mammals Using Honeybee Products
Authors: Hashem Nesrein M. , Hassanein Eman M. , Simal-Gandara Jesus
Published: February 2021

Honeybee products have positive effects on the reproductive performance of mammals. Many honeybee product constituents are biologically active, with antioxidant, antimicrobial, antiviral, anti-inflammatory, immunomodulatory, antifungal, wound-healing, and cardio-protective properties. Honeybee products also improve male and female fertility rates by enhancing gamete cryopreservation, in vitro maturation and fertilization, and embryo development. Previously published studies confirmed their efficacy for alleviating reproductive toxicity caused by contaminants and lifestyle habits that impair overall health and well-being. However, high-dose oral administration of honeybee products may adversely affect the reproductive system, and unfavorable effects were alleviated by treatment cessation. For this reason, this review proposes that bioactive components from bee products can be used as a strategy for improving the reproductive performance and health of mammals.
Clinical Applications of In Vivo and Ex Vivo Confocal Microscopy
Authors: Guida Stefania , Arginelli Federica , Farnetani Francesca , Ciardo Silvana , Bertoni Laura , Manfredini Marco , Zerbinati Nicola , Longo Caterina , Pellacani Giovanni
Published: February 2021

Confocal laser scanning microscopy (CLSM) has been introduced in clinical settings as a tool enabling a quasi-histologic view of a given tissue, without performing a biopsy. It has been applied to many fields of medicine mainly to the skin and to the analysis of skin cancers for both in vivo and ex vivo CLSM. In vivo CLSM involves reflectance mode, which is based on refractive index of cell structures serving as endogenous chromophores, reaching a depth of exploration of 200 μm. It has been proven to increase the diagnostic accuracy of skin cancers, both melanoma and non-melanoma. While histopathologic examination is the gold standard for diagnosis, in vivo CLSM alone and in addition to dermoscopy, contributes to the reduction of the number of excised lesions to exclude a melanoma, and to improve margin recognition in lentigo maligna, enabling tissue sparing for excisions. Ex vivo CLSM can be performed in reflectance and fluorescent mode. Fluorescence confocal microscopy is applied for “real-time” pathological examination of freshly excised specimens for diagnostic purposes and for the evaluation of margin clearance after excision in Mohs surgery. Further prospective interventional studies using CLSM might contribute to increase the knowledge about its application, reproducing real-life settings.
A Novel Approach to the Analysis of the Soil Consolidation Problem by Using Non-Classical Rheological Schemes
Authors: Józefiak Kazimierz , Zbiciak Artur , Brzeziński Karol , Maślakowski Maciej
Published: February 2021

The paper presents classical and non-classical rheological schemes used to formulate constitutive models of the one-dimensional consolidation problem. The authors paid special attention to the secondary consolidation effects in organic soils as well as the soil over-consolidation phenomenon. The systems of partial differential equations were formulated for every model and solved numerically to obtain settlement curves. Selected numerical results were compared with standard oedometer laboratory test data carried out by the authors on organic soil samples. Additionally, plasticity phenomenon and non-classical rheological elements were included in order to take into account soil over-consolidation behaviour in the one-dimensional settlement model. A new way of formulating constitutive equations for the soil skeleton and predicting the relationship between the effective stress and strain or void ratio was presented. Rheological structures provide a flexible tool for creating complex constitutive relationships of soil.
Effective Safety Assessment of Aged Concrete Gravity Dam based on the Reliability Index in a Seismically Induced Site
Authors: Nahar Tahmina Tasnim , Rahman Md Motiur , Kim Dookie
Published: February 2021

The seismic performance of the aged-concrete gravity dam (aged-CGD) by safety assessment based on the reliability-index is the main focal point of this study. Determination of reliability-index has been handled by the site seismic hazard analysis with the help of PSHRisk-tool (developed by the authors) and risk assessment. Incorporated with the uncertainties, the failure probability has been carried out by the IDA and fragility analysis. For the nonlinear finite element model of the CGD, the concrete damage plasticity (CDP) model is adopted. To investigate the aging effect, the hygro-chemo-mechanical model has been taken for different years consideration. Through the failure risk assessment of serviceability and safety level, the target reliability index has been determined here for an existing CGD in Korea. Despite several types of research on the CGD safety assessment, the main novelty of this proposed approach will help the dam operator to check the safety barrier for the aged-CGD. A safety index is investigated by comparing the target reliability-index of the age-CGD with the reliability-index for two potential earthquake levels. However, the proposed approach can implement to check the safety range of any seismic site for any set of earthquakes.
Palladium Nanoparticles Functionalized with PVP-Quercetin Inhibits Cell Proliferation and Activates Apoptosis in Colorectal Cancer Cells
Authors: Piñón-Castillo Hilda Amelia , Martínez-Chamarro Rigoberto , Reyes-Martínez Reyna , Salinas-Vera Yarely M. , Manjarrez-Nevárez Laura A. , Muñoz-Castellanos Laila Nayzzel , López-Camarillo César , Orrantia-Borunda Erasmo
Published: February 2021

Nanotechnology is focused on the development and application of novel nanomaterials with particular physicochemical properties. Palladium nanoparticles (PdNPs) have been used as antimicrobials, antifungals, and photochemicals and for catalytic activity in dye reduction. In the present investigation, we developed and characterized PdNPs as a carrier of quercetin and initiated a study of its effects in colorectal cancer cells. PdNPs were first functionalized with polyvinylpyrrolidone (PVP) and then coupled to quercetin (PdNPs-PVP-Q). Our results showed that quercetin was efficiently incorporated to PdNPs-PVP, as demonstrated using UV/Vis and FT-IR spectroscopy. Using transmission electron microscopy, we demonstrated a reduction in size from 3–14.47 nm of PdNPs alone to 1.8–7.4 nm of PdNPs-PVP and to 2.12–3.14 of PdNPs-PVP-Q, indicating an increase in superficial area in functionalized PdNPs-Q. Moreover, hydrodynamic size studies using dynamic light scattering showed a reduction in size from 2120.33 nm ± 112.53 with PdNPs alone to 129.96 nm ± 6.23 for PdNPs-PVP-Q, suggesting a major reactivity when quercetin is coupled to nanoparticles. X-ray diffraction assays show that the addition of PVP or quercetin to PdNPs does not influence the crystallinity state. Catalytic activity assays of PdNPs-PVP-Q evidenced the chemical reduction of 4-nitrophenol, methyl orange, and methyl blue, thus confirming an electron acceptor capacity of nanoparticles. Finally, biological activity studies using MTT assays showed a significant inhibition (p < 0.05) of cell proliferation of HCT-15 colorectal cancer cells exposed to PdNPs-PVP-Q in comparison to untreated cells. Moreover, treatment with PdNPs-PVP-Q resulted in the apoptosis activation of HCT-15 cells. In conclusion, here we show for the first time the development of PdNPs-PVP-Q and evidence its biological activities through the inhibition of cell proliferation and apoptosis activation in colorectal cancer cells in vitro.
Work Function Tuning in Hydrothermally Synthesized Vanadium-Doped MoO3 and Co3O4 Mesostructures for Energy Conversion Devices
Authors: Dalle Feste Pietro , Crisci Matteo , Barbon Federico , Tajoli Francesca , Salerno Marco , Drago Filippo , Prato Mirko , Gross Silvia , Gatti Teresa , Lamberti Francesco
Published: February 2021

The wide interest in developing green energy technologies stimulates the scientific community to seek, for devices, new substitute material platforms with a low environmental impact, ease of production and processing and long-term stability. The synthesis of metal oxide (MO) semiconductors fulfils these requirements and efforts are addressed towards optimizing their functional properties through the improvement of charge mobility or energy level alignment. Two MOs have rising perspectives for application in light harvesting devices, mainly for the role of charge selective layers but also as light absorbers, namely MoO3 (an electron blocking layer) and Co3O4 (a small band gap semiconductor). The need to achieve better charge transport has prompted us to explore strategies for the doping of MoO3 and Co3O4 with vanadium (V) ions that, when combined with oxygen in V2O5, produce a high work function MO. We report on subcritical hydrothermal synthesis of V-doped mesostructures of MoO3 and of Co3O4, in which a tight control of the doping is exerted by tuning the relative amounts of reactants. We accomplished a full analytical characterization of these V-doped MOs that unambiguously demonstrates the incorporation of the vanadium ions in the host material, as well as the effects on the optical properties and work function. We foresee a promising future use of these materials as charge selective materials in energy devices based on multilayer structures.