bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Analysis of Rubberized Steel Fiber Reinforced Concrete Beams Subjected to Static and Blast Loadings
Authors: Nawar Mahmoud T. , Eisa Ahmed S. , Elshazli Mohamed T. , Ibrahim Yasser E. , El-Zohairy Ayman
Published: March 2024

In recent years, the alarming number of terrorist attacks has highlighted the critical need for extensive research aimed at fortifying structures against explosion-induced loads. However, the insufficient energy absorption and brittleness of conventional concrete make it ineffective in withstanding blast loading, encouraging researchers to explore innovative strategies for augmenting the energy dissipation capabilities of construction materials. This study specifically delves into the incorporation of recycled rubber, a sustainable and environmentally friendly solution to the pressing issue of scrap tire disposal. The primary focus of this research revolves around the integration of rubber recycling and steel fibers into concrete, with the ultimate goal of enhancing the dynamic response of reinforced concrete (RC) beams. This novel approach not only contributes to the structural resilience required for resisting blast impacts, but also aligns with eco-friendly practices by reusing recycled rubber. A meticulous numerical investigation was undertaken to comprehensively assess the static and blast response of these augmented beams. The numerical study involved developing finite element (FE) models using ABAQUS version 6.14 for static implicit analysis and LS-DYNA R11 for blast explicit simulations. The ABAQUS model was validated against previous experimental testing for load–displacement and failure patterns. Similarly, the LS-DYNA model was validated for blast pressure in accordance with UFC-3-340 standards and for material response under blast loading, utilizing existing experimental data. The numerical models were designed to accommodate varying weight percentages of rubber, ranging from 5% to 20%, and a consistent 1.0% incorporation of steel fibers. This comprehensive analysis aims to provide valuable insights into the efficacy of these materials in improving the structural integrity and blast resistance of RC beams, thereby contributing to the development of more secure and sustainable construction practices. By reducing the reinforcement ratio in order to meet the minimum code requirements, it became evident that the failures of the rubberized RC beams tended to exhibit ductility on the tension side under static loading. In addition, the increase in the reinforcement ratio correlated with a higher failure load and decreased deflection. Furthermore, the findings indicated an optimal concrete mixture characterized by improved ductility, energy absorption, and blast load capacity, achieved by combining 5–10% rubber with steel fibers.
Static Robust Design Optimization Using the Stochastic Frontier Method: A Case Study of Pulsed EPD Process on TiO2 Films
Authors: Rezgui Mohamed Ali , Trabelsi Ali , Barbana Nesrine , Ben Youssef Adel , Al-Addous Mohammad
Published: March 2024

This paper aims to optimize a pulsed electrophoretic deposition (EPD) process for TiO2 films. This is accomplished by determining the optimal configuration of the coating parameters from a robust optimization perspective. The experimental study uses a composite central design (CCD) with four control factors, i.e., the initial concentration (x1 in g/L), the deposition time (x2 in s), the duty cycle (x3 in %), and the voltage (x4 in V). The process responses that should all be maximized are the photocatalytic efficiency of the thin film (De) and three critical charges, which characterize the adhesion failure, i.e., LC1: the load at which the first cracks occurred; LC2: the load at which the film starts to delaminate at the edge level of the scratch track; and LC3: the load when the damage of the film exceeds 50%. This paper compares the robust optimization design of the EPD process using two methods: the robust design of processes and products using the stochastic frontier (RDPP-SF) and the surface response and desirability function methods. The findings show that the RDPP-SF method is superior to the response surface–desirability method for the process responses De and LC2 because of non-natural sources of variation; however, both methods perform comparably well while analyzing the LC1 and LC3 responses, which are subjected to pure random variability. The parameters setting for the process robust optimization are met in run 25 (x1 = 14 g/L, x2 = 150 s, x3 = 50%, and x4 40 V).
The Impact of the COVID-19 Pandemic on Esophageal and Gastric Cancer Surgery in Germany: A Four-Year Retrospective Single-Center Study of 287 Patients
Authors: Ibach Marius , Winter Axel , Seika Philippa , Ritschl Paul , Berndt Nadja , Dobrindt Eva , Raakow Jonas , Pratschke Johann , Denecke Christian , Maurer Max Magnus
Published: March 2024

Background: Disruptions to surgical care for cancer patients during the COVID-19 pandemic remain an ongoing debate. This study assesses the effects of the COVID-19 pandemic on perioperative outcomes in a continuous series of surgically treated esophageal and gastric carcinoma patients at a large university hospital in Europe over 48 months. Methods: We conducted a retrospective single-center cohort study at a tertiary referral center. All patients who underwent oncologic esophageal or gastric resection between March 2018 and February 2022 were included in the analysis. The sample was split into a 24 months COVID-19 and an equivalent pre-COVID-19 control period. Outcome variables included caseload, in-hospital mortality, morbidity, treatment course, and disease stage at presentation. Results: Surgeons performed 287 operations, with around two-thirds (62%) of the cohort undergoing esophagectomy and one-third (38%) gastrectomy. The in-hospital mortality was 1% for the COVID-19 and the control periods. Patients did not present at a later disease stage nor did they wait longer for treatment. There was no decrease in caseload, and patients did not suffer from more perioperative complications during COVID-19. Conclusions: Esophageal and gastric carcinoma patients received safe and timely surgical care during the pandemic. Future pandemic protocols may streamline oncologic care towards tertiary referral centers.
Application of ANN in Construction: Comprehensive Study on Identifying Optimal Modifier and Dosage for Stabilizing Marine Clay of Qingdao Coastal Region of China
Authors: Bo Qirui , Liu Junwei , Shang Wenchang , Garg Ankit , Jia Xiaoru , Sun Kaiyue
Published: March 2024

Nowadays, the use of new compound chemical stabilizers to treat marine clay has gained significant attention. However, the complex non-linear relationship between the influencing factors and the unconfined compressive strength of chemically treated marine clay is not clear. In order to study the influence of various factors (dosage, type of stabilizer, curing age) on the unconfined compressive strength of solidified soil during chemical treatment, experiments were performed to determine the unconfined compressive strength of soft marine clay modified with various types of stabilizers. Further, an artificial neural network (ANN) model was used to establish a prediction model based on the unconfined compressive strength test data and to verify the performance. Sensitivity and optimization analyses were further conducted to explore the relative significance of parameters as well as the optimal dosage amount. Research has found that when the content of aluminate cement is 89.5% and the content of curing agent is 30%, the unconfined compressive strength significantly increases after 28 days of solidification, and the change in quicklime content has the greatest effect on the improvement in the unconfined compressive strength. The influence of modifiers on the unconfined compressive strength is in the order: potassium hydroxide > kingsilica > quick lime > bassanite. The values of each factor were obtained when the unconfined compressive strength was the maximum, which provided support for the optimization of the treatment scheme. The analysis of chemical treatment is no longer limited to the linear relationship according to the test results, which proves the feasibility of non-linear relationship analysis based on the artificial neural network.
Myco–Phycobiont Interactions within the “Ramalina farinacea Group”: A Geographical Survey over Europe and Macaronesia
Authors: Moya Patricia , Chiva Salvador , Pazos Tamara , Barreno Eva , Carrasco Pedro , Muggia Lucia , Garrido-Benavent Isaac
Published: March 2024

Ramalina farinacea is a widely distributed epiphytic lichen from the Macaronesian archipelagos to Mediterranean and Boreal Europe. Previous studies have indicated a specific association between R. farinacea and Trebouxia microalgae species. Here, we examined the symbiotic interactions in this lichen and its closest allies (the so-called “R. farinacea group”) across ten biogeographic subregions, spanning diverse macroclimates, analyzing the climatic niche of the primary phycobionts, and discussing the specificity of these associations across the studied area. The most common phycobionts in the “R. farinacea group” were T. jamesii and T. lynnae, which showed a preference for continentality and insularity, respectively. The Canarian endemic R. alisiosae associated exclusively with T. lynnae, while the other Ramalina mycobionts interacted with both microalgae. The two phycobionts exhibited extensive niche overlap in an area encompassing Mediterranean, temperate Europe, and Macaronesian localities. However, T. jamesii occurred in more diverse climate types, whereas T. lynnae preferred warmer and more humid climates, often close to the sea, which could be related to its tolerance to salinity. With the geographical perspective gained in this study, it was possible to show how the association with different phycobionts may shape the ecological adaptation of lichen symbioses.
How Personality Traits Affect Customer Empathy Expression of Social Media Ads and Purchasing Intention: A Psychological Perspective
Authors: Demirci Serhan , Ling Chia-Ju , Lee Dai-Rong , Chen Chien-Wen
Published: March 2024

Consumers’ personality traits significantly influence their perceptions regarding social media advertising. While prior research on consumers’ purchasing intentions in social networking sites advertising has mainly focused on advertising valence antecedents, it is crucial to recognize that consumers’ susceptibility to advertising persuasion, particularly in terms of empathic expression, varies based on a key criterion: whether consumers are driven to attain a specific desired state or are more inclined to avoid an undesirable state. Regulatory Focus Theory (RFT) posits that individuals operate under distinct motivational mechanisms that govern their determination to achieve desired goals, influencing how they process and evaluate advertising messages. In light of RFT, we conducted an online survey with 524 valid responses, utilizing partial least squares (PLS) for research model analysis. The findings revealed that promotion-focused individuals have positively influenced perceptions of social media ad effectiveness (informativeness, ad creativity, perceived relevance, and emotional appeal). In contrast, prevention-focused individuals negatively perceived social media ad effectiveness. Furthermore, this study highlighted that perceived relevance and emotional appeal have a more significant impact on attitudes toward expressing empathy than informativeness and ad creativity.
Book Review: Ajai; Rimjhim, B. Desertification and Land Degradation: Concept to Combating; Routledge: Abingdon, UK, 2022; ISBN: 978-0-8153-6172-5
Authors: Zdruli Pandi
Published: March 2024

Desertification and land degradation (DLD) are some of the most silent, consistent, and dangerous threats to mankind [...]
Comparison of Compact and Decentralized Urban Development Pathways for Flood Mitigation in Urbanizing Deltas—Guangzhou in the Pearl River Delta as a Case Study
Authors: Lin Weibin , Sun Yimin , Nijhuis Steffen
Published: March 2024

Floods are common and inevitable natural disasters. Achieve Sustainable Development Goal (SDG) 11.5 is a critical challenge for coastal cities, especially those in deltaic lowlands such as in the case of Guangzhou, China. Regarding the spatial planning and design of such urban regions, it is crucial to study the impacts of flooding in compact or decentralized spatial development pathways. This reinforces the understanding of the relationship between strategic decisions for spatial planning and flood mitigation. However, the lack of a computer model to assess spatial evolution paths is a significant limitation. The non-dominated Sorting Genetic Algorithm II (NSGA-II) explores the possibility of a compact built-up land layout in 2030. The results showed that, concerning the 2030 decentralized scenario, the 2030 compact scenario presents a large increase in the integrated fitness function value from 0.618 to 0.771 (the increase is equivalent to 0.153 or about 24.75%). In addition, different development scenarios were constructed by setting different target weights. Compared to the decentralized scenario results, the fitness function values of the optimization results of each scenario showed better results at different levels. They could also serve as a reference for other similar coastal areas to achieve SDG 11.5 by 2030.
Bis(2,2,6,6-tetramethyl-1-(λ1-oxidaneyl)piperidin-4-yl) 3,3′-((2-hydroxyethyl)azanediyl)dipropionate
Authors: Kustin Roman P. , Levin Oleg V. , Filippova Sofia S. , Alekseeva Elena V.
Published: March 2024

TEMPO-containing conductive polymers are used in organic electronics due to their electrochemical properties. One of significant limitations in developing such materials is the structural modification by several TEMPO moieties. Here, we report a synthesis of the first-generation dendrimer containing two TEMPO fragments, bis(2,2,6,6-tetramethyl-1-(λ1-oxidaneyl)piperidin-4-yl) 3,3′-((2-hydroxyethyl)azanediyl)dipropionate, that can be implemented into a conductive polymer structure. The resulting product was characterized using 1H and 13C nuclear magnetic resonance spectroscopy (NMR) and high-resolution mass spectroscopy (HRMS).
Special Issue “Recent Advances of Discrete Optimization and Scheduling”
Authors: Lazarev Alexander A. , Werner Frank , Lin Bertrand M. T.
Published: March 2024

This Special Issue of the journal Mathematics is dedicated to new results on the topic of discrete optimization and scheduling [...]
System for Analysis of Wind Collocations (SAWC): A Novel Archive and Collocation Software Application for the Intercomparison of Winds from Multiple Observing Platforms
Authors: Lukens Katherine E. , Garrett Kevin , Ide Kayo , Santek David , Hoover Brett , Huber David , Hoffman Ross N. , Liu Hui
Published: March 2024

Accurate atmospheric 3D wind observations are one of the top priorities for the global scientific community. To address this requirement, and to support researchers’ needs to acquire and analyze wind data from multiple sources, the System for Analysis of Wind Collocations (SAWC) was jointly developed by NOAA/NESDIS/STAR, UMD/ESSIC/CISESS, and UW-Madison/CIMSS. SAWC encompasses the following: a multi-year archive of global 3D winds observed by Aeolus, sondes, aircraft, stratospheric superpressure balloons, and satellite-derived atmospheric motion vectors, archived and uniformly formatted in netCDF for public consumption; identified pairings between select datasets collocated in space and time; and a downloadable software application developed for users to interactively collocate and statistically compare wind observations based on their research needs. The utility of SAWC is demonstrated by conducting a one-year (September 2019–August 2020) evaluation of Aeolus level-2B (L2B) winds (Baseline 11 L2B processor version). Observations from four archived conventional wind datasets are collocated with Aeolus. The recommended quality controls are applied. Wind comparisons are assessed using the SAWC collocation application. Comparison statistics are stratified by season, geographic region, and Aeolus observing mode. The results highlight the value of SAWC’s capabilities, from product validation through intercomparison studies to the evaluation of data usage in applications and advances in the global Earth observing architecture.
Application of Amorphous and Nanocrystalline Soft Magnetic Materials in Balanced-Force-Type Electromagnetic Relay
Authors: Ding Ding , You Jiaxin , Cui Xiangqian , Xue Yutong , Tan Xu , Zhai Guofu
Published: March 2024

The magnetic properties of soft magnetic materials, including their saturation magnetic induction strength and permeability, significantly affect the dynamic characteristics of electromagnetic relays. However, the soft materials most commonly used for relays in the magnetic conductive components of electromagnetic systems, such as electrical pure iron, limit further relay design improvement and optimization to a certain extent. Thus, this paper proposes the use of amorphous and nanocrystalline soft magnetic materials with good high-frequency magnetic properties in magnetic circuits. A wavelet analysis was conducted on the high-frequency components of the coil current while the relay operated, and the corresponding magnetic materials were selected. Considering the challenges in processing amorphous and nanocrystalline materials and collecting test data for the accuracy verification of simulation methods, we prepared a scaled-up prototype for use in dynamic characteristic tests. The simulation method was improved, yielding more accurate simulation results regarding the relay’s dynamic characteristics. On this basis, six replacement schemes using amorphous and nanocrystalline materials were considered. The test results proved that this application could improve the relay’s dynamic characteristics. Finally, a full-size sample with an iron core consisting of nanocrystalline alloy 1K107B was prepared, and the conclusions were verified in tests.
Geochemistry, Sr-Nd Isotope Compositions, and U-Pb Chronology of Apatite from Kimberlite in Wafangdian, North China Craton: Constraints on the Late Magmatic Processes
Authors: Ma Sishun , Wang Ende , Fu Haitao , Fu Jianfei , Men Yekai , You Xinwei , Song Kun , Wan Fanglai , Liu Liguang
Published: March 2024

Diamondiferous kimberlites occur in the Wafangdian area in the eastern part of the North China Craton (NCC). In order to better constrain their magmatic source and emplacement time, we have investigated apatite from two kimberlites, i.e., the #110 dike kimberlite and the #50 root-zone kimberlite by measuring in situ their U–Pb and Sr–Nd isotopic compositions. The crystallization ages of the #110 and #50 apatites are 460.9 ± 16.8 Ma and 455.4 ± 19.3 Ma, respectively. For the #50 apatite, 87Sr/86Sr = 0.70453–0.70613 and εNd(t) = −2.74 to −4.52. For the #110 apatite, 87Sr/86Sr = 0.70394–0.70478 and εNd(t) = −3.46 to −5.65. Based on the similar distribution patterns of the rare earth elements (REEs) and the similar Sr-Nd isotope compositions of the apatite, it is believed that the #110 and #50 kimberlites have the same source region and the kimberlite magmas in Wafangdian were derived from an enriched mantle source (EMI). The primary magmatic composition has little effect on the emplacement pattern. It is more likely that the geological environment played an important role in controlling the retention and removal of volatile components (H2O and CO2). This led to the different evolutionary paths of the kimberlite magma in the later period, resulting in differences in the major element compositions of the apatite. High Sr concentrations may be associated with hydrothermal (H2O-rich fluid) overprinting events in the later magmatic period; the higher light rare earth element (LREE) concentration of the #50 apatite reflects the involvement of the REE3+ + SiO44− ⇔ Ca2+ + PO43− replacement mechanism. Two emplacement patterns of the #110 dike kimberlite (#110 apatite, low Sr, and high Si) and the #50 root-zone (#50 apatite, high Sr, and low Si) kimberlites were identified via major element analysis of the #110 apatite and #50 apatite.
Lithium-, Phosphorus-, and Fluorine-Rich Intrusions and the Phosphate Sequence at Segura (Portugal): A Comparison with Other Hyper-Differentiated Magmas
Authors: Cathelineau Michel , Boiron Marie-Christine , Lecomte Andreï , Martins Ivo , da Silva Ícaro Dias , Mateus Antonio
Published: March 2024

Near the Segura pluton, hyper-differentiated magmas enriched in F, P, and Li migrated through shallowly dipping fractures, which were sub-perpendicular to the schistosity of the host Neoproterozoic to Lower Cambrian metasedimentary series, to form two swarms of low-plunging aplite–pegmatite dykes. The high enrichment factors for the fluxing elements (F, P, and Li) compared with peraluminous granites are of the order of 1.5 to 5 and are a consequence of the extraction of low-viscosity magma from the crystallising melt. With magmatic differentiation, increased P and Li activity yielded the crystallisation of the primary amblygonite–montebrasite series and Fe-Mn phosphates. The high activity of sodium during the formation of the albite–topaz assemblage in pegmatites led to the replacement of the primary phosphates by lacroixite. The influx of external, post-magmatic, and Ca-Sr-rich hydrothermal fluids replaced the initial Li-Na phosphates with phosphates of the goyazite–crandallite series and was followed by apatite formation. Dyke emplacement in metasediments took place nearby the main injection site of the muscovite granite, which plausibly occurred during a late major compression event.
Silver-Loaded Chabazite in Ethanol-to-Hydrocarbon Process—Operando FT-IR and UV-Vis Spectroscopic Studies
Authors: Tarach Karolina A. , Walczyk Anna K. , Kordek Agata , Rogala Oliwia , Góra-Marek Kinga
Published: March 2024

The ethanol dehydration process is studied regarding protonic and Ag-loaded chabazite zeolite in advanced FT-IR and UV-vis operando spectroscopic studies with simultaneous mass spectroscopy and gas chromatography analyses of products. The spectroscopic investigation provides information on the species formed on the surface of catalysts, while mass spectrometry and gas chromatography methods identify the desorbed products. These studies are also supported by spectroscopic, chromatographic, and thermogravimetric analyses of coke species formed over the catalyst’s surface during ethanol conversion. The Ag-chabazite catalyst shows higher selectivity for ethylene and propylene; the slower formation of coke species; and, thus, a longer lifetime.
Design, Synthesis, Biological Evaluation and Molecular Docking of Novel F-18-Labeled Focal Adhesion Kinase Inhibitors as Potential Tumor Radiotracers
Authors: Yang Hailong , Li Ye , Liang Huaju , Cui Chun , Gan Lu , Zhang Huabei
Published: March 2024

Tumor diagnosis, especially at the early stages, holds immense significance. Focal adhesion kinase (FAK) is often highly expressed across various types of tumors, making it a promising target for both therapy and diagnosis. In this study, seven novel inhibitors were designed and synthesized. The inhibitory activity of these compounds against FAK was notably potent, with an IC50 range of 1.27–1968 nM. In particular, compounds 7a and 7c, with IC50 values of 5.59 nM and 1.27 nM, respectively, were radiolabeled with F-18 and then evaluated with S-180 tumor-bearing mice. Subsequently, they exhibited moderate-to-high tumor uptake values, with [18F]7a showing 1.39 ± 0.30%ID/g at 60 min post injection and [18F]7c demonstrating 6.58 ± 0.46%ID/g at 30 min post injection. In addition, the results from docking studies revealed the binding specifics of the studied compounds. Overall, these findings hold the potential to offer valuable guidance for enhancing the development of radiotracers and enzyme inhibitors.
A Virtual Case Presentation Platform: Protocol Study
Authors: Awada Imad Alex , Florea Adina Magda , Scafa-Udriște Alexandru
Published: March 2024

Gaining practical experience is indispensable for medical students. Therefore, when medical students were prevented access to hospitals during the COVID-19 pandemic in Romania, there was an urgent need to find a solution that would allow medical students to develop the skills they would usually develop in hospitals but without the need to be physically present in a hospital. This was the reason behind the idea of developing a Virtual Case Presentation Platform. The platform offers the possibility for medical students to reproduce virtually, in clinically valid scenarios, the diagnostic process and treatment recommendation, as well as the interactions with patients that usually take place in hospitals using natural language through speech and text. On the platform, the students receive valuable feedback from the professors about their performance. In order to reproduce the whole targeted experience for students, without missing anything, before starting the development of the platform, it was mandatory to identify and understand all the aspects that should be covered by the platform. The proposed platform covers the different aspects that have been identified for the diagnostic process and treatment recommendation. It enables medical students to develop essential skills for their future careers as doctors.
A Study of Electroplated Nanoporous Copper Using Aberration-Corrected Transmission Electron Microscopy
Authors: Wang Jianqiang , Wang Jintao , Lv Ziwen , Zhang Luobin , Wang Fengyi , Chen Hongtao , Li Mingyu
Published: March 2024

Nanoporous Cu foam is widely applied in many fields such as the packaging of electronic power devices. In this study, a sandwich-structured Cu-Zn eutectic alloy precursor composed of Cu0.53Zn0.47/Cu5Zn8/Cu0.53Zn0.47 is prepared through electroplating. The surface layer of the precursor, Cu0.53Zn0.47, has a flat surface with numerous grain boundaries, which effectively promotes its dealloying behavior. By contrast, Cu5Zn8 has a porous structure, which promotes the dealloying behavior at the center of the precursor. The dealloying of Cu0.53Zn0.47 is dominated by the coherent surface diffusion of Cu atoms, and the crystal lattice and orientation show no changes before and after dealloying. By contrast, the dealloying behavior of Cu5Zn8 requires the renucleation of Cu crystals; in this process, Cu atoms are transported to the surface of the layer by capillary forces to form clusters, which nucleate and grow.
Structure–Piezoelectric Property Relationships of Thin Films Composed of Electrospun Aligned Poly(vinylidene fluoride) Nanofibers
Authors: Priangga Perdana Putra  , Akasaka Shuichi , Konosu Yuichi , Zhang Shaoling , Tanioka Akihiko , Matsumoto Hidetoshi
Published: March 2024

In the past two decades, many studies on piezoelectric nanofibers (NFs) prepared from poly(vinylidene fluoride) (PVDF) and its copolymers, including single NFs, randomly oriented nonwoven mats, and aligned NFs, have been reported. However, studies on the relationships between the PVDF NF diameter, the orientation of the β-phase crystals inside NFs, and the piezoelectric properties of the NFs are still limited. In this study, the effect of the fiber diameter on the internal molecular packing/orientation and piezoelectric properties of aligned PVDF NF thin films was investigated. Herein, piezoelectric thin films composed of densely packed, uniaxially aligned, PVDF NFs with diameters ranging from 228 to 1315 nm were prepared by means of electrospinning with a rotating collector and successive hot-pressing and poling. The effect of the diameters of PVDF NFs on their internal structures, as well as the piezoelectric properties of the thin films, was investigated. All prepared NFs mainly contained β-phase crystals with a similar total crystallinity. The orientation of the β-phase crystals inside the NFs increased with an increase in the fiber diameter, resulting in an improved transverse piezoelectric coefficient (d31) for the thin films. The output voltage of the prepared thin films reached a maximum of 2.7 V at 104 Hz.
Influence of Anodic Aluminum Oxide Nanostructures on Resistive Humidity Sensing
Authors: Ku Chin-An , Hung Chia-Wei , Chung Chen-Kuei
Published: March 2024

Humidity nanosensors play a vital role in modern technology industries, including weather forecasts, industrial manufacturing, agriculture, food and chemistry storage. In recent years, research on humidity sensors has focused on different materials such as ceramics, polymers, carbon-based materials, semiconductors, MXenes or triboelectric nanogenerators, each with their own advantages and disadvantages. Among them, anodic aluminum oxide (AAO) is a well-known ceramic humidity sensor material with a long history of research and development. AAO humidity sensors offer advantages such as simple manufacturing processes, controllable nanostructures, high thermal stability and biocompatibility. However, traditional AAO fabrication still has disadvantages like high costs and longer process times. Hence, finding a low-cost and efficient method to fabricate AAO for controlling different nanostructures to meet the requirements is consistently a major research topic. From our previous studies, we have studied the relationship between the AAO capacitive humidity sensor and its nanostructures. In this paper, we explore the effect of an AAO nanoporous structure controlled by an anodization voltage of 20–40 V on the resistive-type humidity sensor performance instead of a capacitive one. We efficiently apply one-step hybrid pulse anodization at 25 °C to significantly reduce the processing time compared to the traditional two-step process under 0–10 °C. The AAO nanostructures and their impact on sensor measurements of humidity at 20–80 RH% will be discussed in detail. An electrical resistive sensing mechanism is established for further performance improvement by controlling anodization voltage.
Correction: Crespo et al. Protective Effect of Protocatechuic Acid on TNBS-Induced Colitis in Mice Is Associated with Modulation of the SphK/S1P Signaling Pathway. Nutrients 2017, 9, 288
Authors: Crespo Irene , San-Miguel Beatriz , Mauriz José Luis , Ortiz de Urbina Juan José , Almar Mar , Tuñón María Jesús , González-Gallego Javier
Published: March 2024

In the original publication [...]
“It’s Only a Model”: When Protein Structure Predictions Need Experimental Validation, the Case of the HTLV-1 Tax Protein
Authors: Guillon Christophe , Robert Xavier , Gouet Patrice
Published: March 2024

Human T-cell Leukemia Virus type 1 (HTLV-1) is a human retrovirus responsible for leukaemia in 5 to 10% of infected individuals. Among the viral proteins, Tax has been described as directly involved in virus-induced leukemogenesis. Tax is therefore an interesting therapeutic target. However, its 3D structure is still unknown and this hampers the development of drug-design-based therapeutic strategies. Several algorithms are available that can be used to predict the structure of proteins, particularly with the recent appearance of artificial intelligence (AI)-driven pipelines. Here, we review how the structure of Tax is predicted by several algorithms using distinct modelling strategies. We discuss the consequences for the understanding of Tax structure/function relationship, and more generally for the use of structure models for modular and/or flexible proteins, which are frequent in retroviruses.
The Management of Phaeochromocytomas and Paragangliomas in the Era of Precision Medicine: Where Are We Now? Evidence-Based Systemic Treatment Options and Future Cluster Oriented Perspectives
Authors: Bracigliano Alessandra , Marretta Antonella Lucia , Guerrera Luigi Pio , Simioli Roberto , Clemente Ottavia , Granata Vincenza , Minopoli Anita , Della Vittoria Scarpati Giuseppina , Picozzi Fernanda , Cannella Lucia , Pizzolorusso Antonio , Di Gennaro Francesca , Tafuto Roberto , Sarno Maria Rosaria , Cavalcanti Ernesta , Ribera Dario , Tafuto Salvatore
Published: March 2024

Pheochromocytomas (PCCs) and Paragangliomas (PGLs), commonly known as PPGLs to include both entities, are rare neuroendocrine tumors that may arise in the context of hereditary syndromes or be sporadic. However, even among sporadic PPGLs, identifiable somatic alterations in at least one of the known susceptibility genes can be detected. Therefore, about 3/4 of all PPGL patients can be assigned to one of the three molecular clusters that have been identified in the last years with difference in the underlying pathogenetic mechanisms, biochemical phenotype, metastatic potential, and prognosis. While surgery represents the mainstay of treatment for localized PPGLs, several therapeutic options are available in advanced and/or metastatic setting. However, only few of them hinge upon prospective data and a cluster-oriented approach has not yet been established. In order to render management even more personalized and improve the prognosis of this molecularly complex disease, it is undoubtable that genetic testing for germline mutations as well as genome profiling for somatic mutations, where available, must be improved and become standard practice. This review summarizes the current evidence regarding diagnosis and treatment of PPGLs, supporting the need of a more cluster-specific approach in clinical practice.
Phage Delivery Strategies for Biocontrolling Human, Animal, and Plant Bacterial Infections: State of the Art
Authors: Vila Marta M. D. C. , Balcão Liliane M. N. , Balcão Victor M.
Published: March 2024

This review aims at presenting the main strategies that are currently available for the delivery of bacteriophages to combat bacterial infections in humans, animals, and plants. It can be seen that the main routes for phage delivery are topical, oral, systemic, and airways for humans. In animals, the topical and oral routes are the most used. To combat infections in plant species, spraying the plant’s phyllosphere or drenching the soil are the most commonly used methods. In both phage therapy and biocontrol using phages, very promising results have been obtained so far. However, more experiments are needed to establish forms of treatment and phage doses, among other parameters. Furthermore, in general, there is a lack of specific standards for the use of phages to combat bacterial infections.
First Insights about Antiparasitic and Action Mechanisms of the Antimicrobial Peptide Hepcidin from Salmonids against Caligus rogercresseyi
Authors: Santana Paula A. , Arancibia Camila , Tamayo Laura , Cumillaf Juan Pablo , Roman Tanya , Cárdenas Constanza , Paillan Suarez Cinthya , Álvarez Claudio A. , Guzman Fanny
Published: March 2024

Currently, one of the primary challenges in salmon farming is caligidosis, caused by the copepod ectoparasites Caligus spp. The infection process is determined by the copepod’s ability to adhere to the fish skin through the insertion of its chitin-composed filament. In this study, we examined several antimicrobial peptides previously identified in salmonid mucosal secretions, with a primary focus on their potential to bind to chitin as an initial step. The binding capacity to chitin was tested, with hepcidin and piscidin showing positive results. Further assessments involving cytotoxicity in salmonid cells RTgill-W1, SHK-1, RTS-11, and RT-gut indicated that the peptides did not adversely affect cell viability. However, hemolysis assays unveiled the hemolytic capacity of piscidin at lower concentrations, leading to the selection of hepcidin for antiparasitic assays. The results demonstrated that the nauplius II stage of C. rogercresseyi exhibited higher susceptibility to hepcidin treatments, achieving a 50% reduction in parasitic involvement at 50 µM. Utilizing fluorescence and scanning electron microscopy, we observed the localization of hepcidin on the surface of the parasite, inducing significant spherical protuberances along the exoskeleton of C. rogercresseyi. These findings suggest that cysteine-rich AMPs derived from fish mucosa possess the capability to alter the development of the chitin exoskeleton in copepod ectoparasites, making them therapeutic targets to combat recurrent parasitic diseases in salmon farming.
Responses of Three Pedicularis Species to Geological and Climatic Changes in the Qinling Mountains and Adjacent Areas in East Asia
Authors: Zhang Qijing , Lu Zhaoping , Guo Mingchen , Kang Jia , Li Jia , He Xiaojing , Wu Jiayi , Liu Ruihang , Dang Jiaxin , Li Zhonghu
Published: March 2024

The Qinling Mountains in East Asia serve as the geographical boundary between the north and south of China and are also indicative of climatic differences, resulting in rich ecological and species diversity. However, few studies have focused on the responses of plants to geological and climatic changes in the Qinling Mountains and adjacent regions. Therefore, we investigated the evolutionary origins and phylogenetic relationships of three Pedicularis species in there to provide molecular evidence for the origin and evolution of plant species. Ecological niche modeling was used to predict the geographic distributions of three Pedicularis species during the last interglacial period, the last glacial maximum period, and current and future periods, respectively. Furthermore, the distribution patterns of climate fluctuations and the niche dynamics framework were used to assess the equivalence or difference of niches among three Pedicularis species. The results revealed that the divergence of three Pedicularis species took place in the Miocene and Holocene periods, which was significantly associated with the large-scale uplifts of the Qinling Mountains and adjacent regions. In addition, the geographic distributions of three Pedicularis species have undergone a northward migration from the past to the future. The most important environmental variables affecting the geographic distributions of species were the mean diurnal range and annual mean temperature range. The niche divergence analysis suggested that the three Pedicularis species have similar ecological niches. Among them, P. giraldiana showed the highest niche breadth, covering nearly all of the climatic niche spaces of P. dissecta and P. bicolor. In summary, this study provides novel insights into the divergence and origins of three Pedicularis species and their responses to climate and geological changes in the Qinling Mountains and adjacent regions. The findings have also provided new perspectives for the conservation and management of Pedicularis species.
The Role of Soil Microbial Consortia in Sustainable Cereal Crop Residue Management
Authors: Shamshitov Arman , Kadžienė Gražina , Supronienė Skaidrė
Published: March 2024

The global escalation in cereal production, essential to meet growing population demands, simultaneously augments the generation of cereal crop residues, estimated annually at approximately 3107 × 106 Mg/year. Among different crop residue management approaches, returning them to the soil can be essential for various ecological benefits, including nutrient recycling and soil carbon sequestration. However, the recalcitrant characteristics of cereal crop residues pose significant challenges in their management, particularly in the decomposition rate. Therefore, in this review, we aim to summarize the influence of different agricultural practices on enhancing soil microbial decomposer communities, thereby effectively managing cereal crop residues. Moreover, this manuscript provides indirect estimates of cereal crop residue production in Northern Europe and Lithuania, and highlights the diverse roles of lignocellulolytic microorganisms in the decomposition process, with a particular focus on enzymatic activities. This review bridges the knowledge gap and indicates future research directions concerning the influence of agricultural practices on cereal crop residue-associated microbial consortia.
Temporal and Within-Sporophyte Variations in Triphenyltin Chloride (TPTCL) and Its Degradation Products in Cultivated Undaria pinnatifida
Authors: Ren Xingyue , Zhang Yuanyuan , Gao Xu , Gong Qingli , Li Jingyu
Published: March 2024

Undaria pinnatifida can effectively deal with organotin pollution through its excellent accumulation and degradation capabilities found under laboratory conditions. However, nothing is known regarding its accumulation, degradation performance, and related impact factors in the wild farming area. In this study, we monitored triphenyltin chloride (TPTCL) contents and degradation products in different algal parts (blades, stipes, sporophylls, and holdfasts) of cultivated U. pinnatifida from December 2018 to May 2019. Our results showed that sporophytes had an accumulation and degradation capacity for TPTCL. The TPTCL contents and degradation products varied with the algal growth stages and algal parts. TPTCL accumulated in the blades at the growth stage and the blades, stipes, sporophylls, and holdfasts at the mature stage. The TPTCL content among algal parts was blades (74.92 ± 2.52 μg kg−1) > holdfasts (62.59 ± 1.42 μg kg−1) > sporophylls (47.24 ± 1.41 μg kg−1) > stipes (35.53 ± 0.55 μg kg−1). The primary degradation product DPTCL accumulated only in the blades at any stage, with a concentration of 69.30 ± 3.89 μg kg−1. The secondary degradation product MPTCL accumulated in the blades at the growth stage and in the blades, stipe, and sporophyll at the mature stage. The MPTCL content among algal parts was blades (52.80 ± 3.48 μg kg−1) > sporophylls (31.08 ± 1.53 μg kg−1) > stipes (20.44 ± 0.85 μg kg−1). The accumulation pattern of TPTCL and its degradation products seems closely related to nutrient allocation in U. pinnatifida. These results provide the basis for applying cultivated U. pinnatifida in the bioremediation of organotin pollution and the food safety evaluation of edible algae.
Dissecting the Genetic Basis of Yield Traits and Validation of a Novel Quantitative Trait Locus for Grain Width and Weight in Rice
Authors: Yin Man , Tong Xiaohong , Yang Jinyu , Cheng Yichen , Zhou Panpan , Li Guan , Wang Yifeng , Ying Jiezheng
Published: March 2024

Grain yield in rice is a complex trait and it is controlled by a number of quantitative trait loci (QTL). To dissect the genetic basis of rice yield, QTL analysis for nine yield traits was performed using an F2 population containing 190 plants, which was developed from a cross between Youyidao (YYD) and Sanfenhe (SFH), and each plant in the population evaluated with respect to nine yield traits. In this study, the correlations among the nine yield traits were analyzed. The grain yield per plant positively correlated with six yield traits, except for grain length and grain width, and showed the highest correlation coefficient of 0.98 with the number of filled grains per plant. A genetic map containing 133 DNA markers was constructed and it spanned 1831.7 cM throughout 12 chromosomes. A total of 36 QTLs for the yield traits were detected on nine chromosomes, except for the remaining chromosomes 5, 8, and 9. The phenotypic variation was explained by a single QTL that ranged from 6.19% to 36.01%. Furthermore, a major QTL for grain width and weight, qGW2-1, was confirmed to be newly identified and was narrowed down to a relatively smaller interval of about ~2.94-Mb. Collectively, we detected a total of 36 QTLs for yield traits and a major QTL, qGW2-1, was confirmed to control grain weight and width, which laid the foundation for further map-based cloning and molecular design breeding in rice.
Withania somnifera (L.) Dunal, a Potential Source of Phytochemicals for Treating Neurodegenerative Diseases: A Systematic Review
Authors: Lerose Valentina , Ponticelli Maria , Benedetto Nadia , Carlucci Vittorio , Lela Ludovica , Tzvetkov Nikolay T. , Milella Luigi
Published: March 2024

Withania somnifera (L.) Dunal is a medicinal plant belonging to the traditional Indian medical system, showing various therapeutic effects such as anti-cancer, anti-inflammatory, anti-microbial, anti-diabetic, and hepatoprotective activity. Of great interest is W. somnifera’s potential beneficial effect against neurodegenerative diseases, since the authorized medicinal treatments can only delay disease progression and provide symptomatic relief and are not without side effects. A systematic search of PubMed and Scopus databases was performed to identify preclinical and clinical studies focusing on the applications of W. somnifera in preventing neurodegenerative diseases. Only English articles and those containing the keywords (Withania somnifera AND “neurodegenerative diseases”, “neuroprotective effects”, “Huntington”, “Parkinson”, “Alzheimer”, “Amyotrophic Lateral Sclerosis”, “neurological disorders”) in the title or abstract were considered. Reviews, editorials, letters, meta-analyses, conference papers, short surveys, and book chapters were not considered. Selected articles were grouped by pathologies and summarized, considering the mechanism of action. The quality assessment and the risk of bias were performed using the Cochrane Handbook for Systematic Reviews of Interventions checklist. This review uses a systematic approach to summarize the results from 60 investigations to highlight the potential role of W. somnifera and its specialized metabolites in treating or preventing neurodegenerative diseases.
Identification and Characterization of the AREB/ABF Gene Family in Three Orchid Species and Functional Analysis of DcaABI5 in Arabidopsis
Authors: Xie Xi , Lin Miaoyan , Xiao Gengsheng , Wang Qin , Li Zhiyong
Published: March 2024

AREB/ABF (ABA response element binding) proteins in plants are essential for stress responses, while our understanding of AREB/ABFs from orchid species, important traditional medicinal and ornamental plants, is limited. Here, twelve AREB/ABF genes were identified within three orchids’ complete genomes and classified into three groups through phylogenetic analysis, which was further supported with a combined analysis of their conserved motifs and gene structures. The cis-element analysis revealed that hormone response elements as well as light and stress response elements were widely rich in the AREB/ABFs. A prediction analysis of the orchid ABRE/ABF-mediated regulatory network was further constructed through cis-regulatory element (CRE) analysis of their promoter regions. And it revealed that several dominant transcriptional factor (TF) gene families were abundant as potential regulators of these orchid AREB/ABFs. Expression profile analysis using public transcriptomic data suggested that most AREB/ABF genes have distinct tissue-specific expression patterns in orchid plants. Additionally, DcaABI5 as a homolog of ABA INSENSITIVE 5 (ABI5) from Arabidopsis was selected for further analysis. The results showed that transgenic Arabidopsis overexpressing DcaABI5 could rescue the ABA-insensitive phenotype in the mutant abi5. Collectively, these findings will provide valuable information on AREB/ABF genes in orchids.