bluuebunny/crossref_metadata_2025 huggingface dataset:
CgNPG1 as a Novel Pathogenic Gene of Colletotrichum gloeosporioides From Hevea brasiliensis in Mycelial Growth, Conidiation, and the Invasive Structures Development
Authors: Liang Chen , Zhang Bei , Zhou Yun , Yin Hongyan , An Bang , Lin Daozhe , He Chaozu , Luo Hongli
Published: March 2021

The rubber tree (Hevea brasiliensis) is a tropical perennial crop for the primary source of natural rubber. Colletotrichum gloeosporioides from Hevea brasiliensis (C. gloeosporioides Hb) and Colletotrichum acutatum from Hevea brasiliensis (C. acutatum Hb) are the causal agents of rubber tree anthracnose and lead to serious loss of natural rubber production. Inoculation tests showed that C. gloeosporioides Hb possessed higher pathogenicity than C. acutatum Hb to the rubber tree. Genomic analysis revealed that an unknown gene, named CgNPG1 (a Novel Pathogenic Gene 1), was presented in the genome of C. gloeosporioides Hb but not identified in C. acutatum Hb. CgNPG1 was predicted to encode a small secretory protein without any conserved domain. To investigate the functions of CgNPG1 in C. gloeosporioides Hb and in C. acutatum Hb, the gene deletion and overexpression mutants were generated. The phenotype analysis showed that deletion of CgNPG1 led to changed conidia morphology, decreased mycelial growth, conidiation, conidia germination rate, appressorium formation rate, and pathogenicity of C. gloeosporioides Hb to the rubber tree. Meanwhile, heterogeneous expression of CgNPG1 in C. acutatum Hb significantly changed the conidia morphology and improved the mycelial growth rate, conidiation, conidia germination rate, appressorium formation rate, and the pathogenicity of C. acutatum Hb to the rubber tree. Consistently, CgNPG1 increased the expression level of CaCRZ1 and CaCMK1 in C. acutatum Hb. These data suggested that CgNPG1 contributed to mycelial growth, conidiation, the development of invasive structures, and the pathogenicity of Colletotrichum to the rubber tree, which might be related to the modulation of CaCRZ1 and mitogen-activated protein kinase CMK1. Our results provided new insight into CgNPG1 in regulating growth and pathogenicity of the Colletotrichum spp.
Systematic Profiling of Alternative Splicing Events in Ovarian Cancer
Authors: Liu Jia , Lv Dekang , Wang Xiaobin , Wang Ruicong , Li Xiaodong
Published: March 2021

Alternative splicing (AS) is significantly related to the development of tumor and the clinical outcome of patients. In this study, our aim was to systematically analyze the survival-related AS signal in ovarian serous cystadenocarcinoma (OV) and estimate its prognostic validity in 48,049 AS events out of 21,854 genes. We studied 1,429 AS events out of 1,125 genes, which were significantly related to the overall survival (OS) in patients with OV. We established alternative splicing features on the basis of seven AS events and constructed a new comprehensive prognostic model. Kaplan-Meier curve analysis showed that seven AS characteristics and comprehensive prognostic models could strongly stratify patients with ovarian cancer and make them distinctive prognosis. ROC analysis from 0.781 to 0.888 showed that these models were highly efficient in distinguishing patient survival. We also verified the prognostic characteristics of these models in a testing cohort. In addition, uni-variate and multivariate Cox analysis showed that these models were superior independent risk factors for OS in patients with OV. Interestingly, AS events and splicing factor (SFs) networks revealed an important link between these prognostic alternative splicing genes and splicing factors. We also found that the comprehensive prognosis model signature had higher prediction ability than the mRNA signature. In summary, our study provided a possible prognostic prediction model for patients with OV and revealed the splicing network between AS and SFs, which could be used as a potential predictor and therapeutic target for patients with OV.
Prognostic Immune-Related Analysis Based on Differentially Expressed Genes in Left- and Right-Sided Colon Adenocarcinoma
Authors: Guo Jun-Nan , Li Ming-Qi , Deng Shen-Hui , Chen Chen , Ni Yin , Cui Bin-Bin , Liu Yan-Long
Published: March 2021

Background

Colon adenocarcinoma (COAD) can be divided into left-sided and right-sided COAD (LCCs and RCCs, respectively). They have unique characteristics in various biological aspects, particularly immune invasion and prognosis. The purpose of our study was to develop a prognostic risk scoring model (PRSM) based on differentially expressed immune-related genes (IRGs) between LCCs and RCCs, therefore the prognostic key IRGs could be identified.

Methods

The gene sets and clinical information of COAD patients were derived from TCGA and GEO databases. The comparison of differentially expressed genes (DEGs) of LCCs and RCCs were conducted with appliance of “Limma” analysis. The establishment about co-expression modules of DEGs related with immune score was conducted by weighted gene co-expression network analysis (WGCNA). Furthermore, we screened the module genes and completed construction of gene pairs. The analysis of the prognosis and the establishment of PRSM were performed with univariate- and lasso-Cox regression. We employed the PRSM in the model group and verification group for the purpose of risk group assignment and PRSM accuracy verification. Finally, the identification of the prognostic key IRGs was guaranteed by the adoption of functional enrichment, “DisNor” and protein-protein interaction (PPI).

Results

A total of 215 genes were screened out by differential expression analysis and WGCNA. A PRSM with 16 immune-related gene pairs (IRGPs) was established upon the genes pairing. Furthermore, we confirmed that the risk score was an independent factor for survival by univariate- and multivariate-Cox regression. The prognosis of high-risk group in model group (P < 0.001) and validation group (P = 0.014) was significantly worse than that in low-risk group. Treg cells (P < 0.001) and macrophage M0 (P = 0.015) were highly expressed in the high-risk group. The functional analysis indicated that there was significant up-regulation with regard of lymphocyte and cytokine related terms in low-risk group. Finally, we identified five prognostic key IRGs associated with better prognosis through PPI and prognostic analysis, including IL2RB, TRIM22, CIITA, CXCL13, and CXCR6.

Conclusion

Through the analysis and screening of the DEGs between LCCs and RCCs, we constructed a PRSM which could predicate prognosis of LCCs and RCCs, and five prognostic key IRGs were identified as well. Therefore, the basis for identifying the benefits of immunotherapy and immunomodulatory was built.
Correlation Between In Vivo Reflectance Confocal Microscopy and Horizontal Histopathology in Skin Cancer: A Review
Authors: Broggi Giuseppe , Verzì Anna Elisa , Caltabiano Rosario , Micali Giuseppe , Lacarrubba Francesco
Published: March 2021

In dermatopathological daily practice, vertical histopathology sections are classically used to analyze skin biopsies. Conversely, horizontal histopathological sections are currently used for the diagnosis of some types of alopecia. In the last years the morphological findings obtained by horizontal histopathology have been correlated to those obtained by in vivo reflectance confocal microscopy which provides the same “point of view” of the skin. This review paper emphasizes the strong matching and correlation between reflectance confocal microscopy images and horizontal histopathology in cutaneous neoplasms, further demonstrating the strong reliability of this innovative, non-invasive technique in the management of skin tumors.
Case Report: A Case of Glioblastoma in a Patient With Haberland Syndrome
Authors: Ferranti Silvia , Sardi Iacopo , Guidi Milena , Lembo Chiara , Grosso Salvatore
Published: March 2021

Haberland syndrome or encephalocraniocutaneous lipomatosis is a rare ectomesodermal dysgenesis defined by the triad including ocular, skin, and central nervous system involvement, which is commonly unilateral. This disorder is attributed to a post-zygotic mutation responsible for a neural tube and neural crest dysgenesis. We report the case of a 15-year-old female with Haberland syndrome with pharmacoresistant epilepsy who developed a World Health Organization-grade IV glioblastoma. This is the first case of pediatric glioblastoma associated with Haberland syndrome. The previously reported pediatric cases included benign brain tumors. To our knowledge, this is the fifth case of brain tumor associated with encephalocraniocutaneous lipomatosis and the second case of glioblastoma associated with this syndrome. The hypothesis that Haberland syndrome is associated with an increased risk of tumor development is intriguing, although the rarity of the condition is nowadays preventing us from drawing definitive conclusions about this potential link between the two entities. Further studies are needed to establish the real relationship between encephalocraniocutaneous lipomatosis and the risk of brain tumors.
UGT1A1 rs4148323 A Allele is Associated With Increased 2-Hydroxy Atorvastatin Formation and Higher Death Risk in Chinese Patients With Coronary Artery Disease
Authors: Lei He-Ping , Qin Min , Cai Li-Yun , Wu Hong , Tang Lan , Liu Ju-E , Deng Chun-Yu , Liu Yi-Bin , Zhu Qian , Li Han-Ping , Hu Wei , Yang Min , Zhu Yi-Zhun , Zhong Shi-Long
Published: March 2021

It is widely accepted that genetic polymorphisms impact atorvastatin (ATV) metabolism, clinical efficacy, and adverse events. The objectives of this study were to identify novel genetic variants influencing ATV metabolism and outcomes in Chinese patients with coronary artery disease (CAD). A total of 1079 CAD patients were enrolled and followed for 5 years. DNA from the blood and human liver tissue samples were genotyped using either Global Screening Array-24 v1.0 BeadChip or HumanOmniZhongHua-8 BeadChip. Concentrations of ATV and its metabolites in plasma and liver samples were determined using a verified ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS) method. The patients carrying A allele for the rs4148323 polymorphism (UGT1A1) showed an increase in 2-hydroxy ATV/ATV ratio (p = 1.69E−07, false discovery rate [FDR] = 8.66E−03) relative to the value in individuals without the variant allele. The result was further validated by an independent cohort comprising an additional 222 CAD patients (p = 1.08E−07). Moreover, the rs4148323 A allele was associated with an increased risk of death (hazard ratio [HR] 1.774; 95% confidence interval [CI], 1.031–3.052; p = 0.0198). In conclusion, our results suggested that the UGT1A1 rs4148323 A allele was associated with increased 2-hydroxy ATV formation and was a significant death risk factor in Chinese patients with CAD.
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus
Authors: Amoateng Patrick , Tagoe Thomas A. , Karikari Thomas K. , Kukuia Kennedy K. E , Osei-Safo Dorcas , Woode Eric , Frenguelli Bruno G. , Kombian Samuel B.
Published: March 2021

Extracts of the tropical Cinderella plantSynedrella nodifloraare used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal slice preparation from rats, the ability of the extract to depress excitatory synaptic transmission andin vitroseizure activity were investigated. Bath perfusion of the hydro-ethanolic extract ofSynedrella nodiflora(SNE) caused a concentration-dependent depression of evoked field excitatory postsynaptic potentials (fEPSPs) recorded extracellularly in the CA1 region of the hippocampus with maximal depression of about 80% and an estimated IC50of 0.06 mg/ml. The SNE-induced fEPSP depression was accompanied by an increase in paired pulse facilitation. The fEPSP depression only recovered partially after 20 min washing out. The effect of SNE was not stimulus dependent as it was present even in the absence of synaptic stimulation. Furthermore, it did not show desensitization as repeat application after 10 min washout produced the same level of fEPSP depression as the first application. The SNE effect on fEPSPs was not via adenosine release as it was neither blocked nor reversed by 8-CPT, an adenosine A1receptor antagonist. In addition, SNE depressedin vitroseizures induced by zero Mg2+and high K+-containing artificial cerebrospinal fluid (aCSF) in a concentration-dependent manner. The results show that SNE depresses fEPSPs and spontaneous bursting activity in hippocampal neurons that may underlie its ability to abort convulsive activity in persons with epilepsy.
Mechanism and Basis of Traditional Chinese Medicine Against Obesity: Prevention and Treatment Strategies
Authors: Zhang Chang-hua , Sheng Jun-qing , Xie Wei-hua , Luo Xiao-quan , Xue Ya-nan , Xu Guo-Liang , Chen Chen
Published: March 2021

In the last few decades, the incidences of obesity and related metabolic disorders worldwide have increased dramatically. Major pathophysiology of obesity is termed “lipotoxicity” in modern western medicine (MWM) or “dampness-heat” in traditional Chinese medicine (TCM). “Dampness-heat” is a very common and critically important syndrome to guild clinical treatment in TCM. However, the pathogenesis of obesity in TCM is not fully clarified, especially by MWM theories compared to TCM. In this review, the mechanism underlying the action of TCM in the treatment of obesity and related metabolic disorders was thoroughly discussed, and prevention and treatment strategies were proposed accordingly. Hypoxia and inflammation caused by lipotoxicity exist in obesity and are key pathophysiological characteristics of “dampness-heat” syndrome in TCM. “Dampness-heat” is prevalent in chronic low-grade systemic inflammation, prone to insulin resistance (IR), and causes variant metabolic disorders. In particular, the MWM theories of hypoxia and inflammation were applied to explain the “dampness-heat” syndrome of TCM, and we summarized and proposed the pathological path of obesity: lipotoxicity, hypoxia or chronic low-grade inflammation, IR, and metabolic disorders. This provides significant enrichment to the scientific connotation of TCM theories and promotes the modernization of TCM.
An EEG Analysis of Honorification in Japanese: Human Hierarchical Relationships Coded in Language
Authors: Tokimoto Shingo , Miyaoka Yayoi , Tokimoto Naoko
Published: March 2021

This study examines the neural substrate of the understanding of human relationships in verbal communication with Japanese honorific sentences as experimental materials. We manipulated two types of Japanese verbs specifically used to represent respect for others, i.e., exalted and humble verbs, which represent respect for the person in the subject and the person in the object, respectively. We visually presented appropriate and anomalous sentences containing the two types of verbs and analyzed the electroencephalogram elicited by the verbs. We observed significant parietal negativity at a latency of approximately 400 ms for anomalous verbs compared with appropriate verbs. This parietal negativity could be a manifestation of the pragmatic process used to integrate the linguistic forms with the human relationships represented in the sentences. The topographies of these event-related potentials (ERPs) corresponded well with those of ERPs for two second-person pronouns in Chinese (plain ni and respectful nin). This correspondence suggests that the pragmatic integration process in honorific expressions is cross-linguistically common in part. Furthermore, we assessed the source localization by means of independent component (IC) analysis and dipole fitting and observed a significant difference in ERP between the honorific and control sentences in the IC cluster centered in the precentral gyrus and in the cluster centered in the medial part of the occipital lobe, which corresponded well with the functional magnetic resonance imaging findings for Japanese honorification. We also found several significant differences in the time-frequency analyses for the medial occipital cluster. These significant differences in the medial occipital cluster suggested that the circuit of the theory of mind was involved in the processing of Japanese honorification. Our results suggest that pragmatic and syntactic processing are performed in parallel because the person to be respected must fulfill the grammatical function appropriate for the honorific verb.
Why Do We Take Risks? Perception of the Situation and Risk Proneness Predict Domain-Specific Risk Taking
Authors: de-Juan-Ripoll Carla , Chicchi Giglioli Irene Alice , Llanes-Jurado Jose , Marín-Morales Javier , Alcañiz Mariano
Published: March 2021

Risk taking (RT) is a component of the decision-making process in situations that involve uncertainty and in which the probability of each outcome – rewards and/or negative consequences – is already known. The influence of cognitive and emotional processes in decision making may affect how risky situations are addressed. First, inaccurate assessments of situations may constitute a perceptual bias in decision making, which might influence RT. Second, there seems to be consensus that a proneness bias exists, known as risk proneness, which can be defined as the propensity to be attracted to potentially risky activities. In the present study, we take the approach that risk perception and risk proneness affect RT behaviours. The study hypothesises that locus of control, emotion regulation, and executive control act as perceptual biases in RT, and that personality, sensation seeking, and impulsivity traits act as proneness biases in RT. The results suggest that locus of control, emotion regulation and executive control influence certain domains of RT, while personality influences in all domains except the recreational, and sensation seeking and impulsivity are involved in all domains of RT. The results of the study constitute a foundation upon which to build in this research area and can contribute to the increased understanding of human behaviour in risky situations.
Studying Music During the Coronavirus Pandemic: Conditions of Studying and Health-Related Challenges
Authors: Rosset Magdalena , Baumann Eva , Altenmüller Eckart
Published: March 2021

Objective

The coronavirus pandemic affects all areas of life. Performing arts and music studies have also experienced considerable changes, with university closures and a fluctuating return to normal and more limited operations. Prior studies detail the impact of the pandemic on college students, but we do not yet know what specific consequences it has for music students. The aim of this study is to examine the impact of the coronavirus pandemic on music students’ health, practicing behavior, and everyday life.

Methods

In July 2020, we conducted an online survey of 80 students enrolled in performance and music education programs at a German music university and compared these data with data from a survey of 75 students at the same music university carried out in summer 2019.

Results

The survey revealed that the coronavirus pandemic led to a decrease in practicing hours and an increase of stressful thoughts and feelings. Students were mostly satisfied with Corona-measures taken by the university. Of analyzed determinants, only general fear of health problems was identified as a significant predictor of mental health status. Mental health status did not significantly differ between students surveyed in 2019 and 2020.

Conclusion

Knowledge about the specific challenges the pandemic poses for music students can help conservatories to better respond to the needs of their students. Specifically, this study will inform future measures supporting music students in coping with difficult situations like a pandemic.
GABA, Glutamate and Neural Activity: A Systematic Review With Meta-Analysis of Multimodal 1H-MRS-fMRI Studies
Authors: Kiemes Amanda , Davies Cathy , Kempton Matthew J. , Lukow Paulina B. , Bennallick Carly , Stone James M. , Modinos Gemma
Published: March 2021

Multimodal neuroimaging studies combining proton magnetic resonance spectroscopy (1H-MRS) to quantify GABA and/or glutamate concentrations and functional magnetic resonance imaging (fMRI) to measure brain activity non-invasively have advanced understanding of how neurochemistry and neurophysiology may be related at a macroscopic level. The present study aimed to perform a systematic review and meta-analysis of available studies examining the relationship between 1H-MRS glutamate and/or GABA levels and task-related fMRI signal in the healthy brain. Ovid (Medline, Embase, and PsycINFO) and Pubmed databases were systematically searched to identify articles published until December 2019. The primary outcome of interest was the association between resting levels of glutamate or GABA and task-related fMRI. Fifty-five papers were identified for inclusion in the systematic review. A further 22 studies were entered into four separate meta-analyses. These meta-analyses found evidence of significant negative associations between local GABA levels and (a) fMRI activation to visual tasks in the occipital lobe, and (b) activation to emotion processing in the medial prefrontal cortex (mPFC)/anterior cingulate cortex (ACC). However, there was no significant association between mPFC/ACC glutamate levels and fMRI activation to cognitive control tasks or to emotional processing, with the relationship to emotion processing related neural activity narrowly missing significance. Moreover, our systematic review also found converging evidence of negative associations between GABA levels and local brain activity, and positive associations between glutamate levels and distal brain activity, outside of the 1H-MRS sampling region. Albeit less consistently, additional relationships between GABA levels and distal brain activity and between glutamate levels and local brain activity were found. It remains unclear if the absence of effects for other brain regions and other cognitive-emotional domains reflects study heterogeneity or potential confounding effects of age, sex, or other unknown factors. Advances in 1H-MRS methodology as well as in the integration of 1H-MRS readouts with other imaging modalities for indexing neural activity hold great potential to reveal key aspects of the pathophysiology of mental health disorders involving aberrant interactions between neurochemistry and neurophysiology such as schizophrenia.
Aerodynamic and Aeroacoustic Analysis of a Harmonically Morphing Airfoil Using Dynamic Meshing
Authors: Abdessemed Chawki , Bouferrouk Abdessalem , Yao Yufeng
Published: March 2021

This work explores the aerodynamic and aeroacoustic responses of an airfoil fitted with a harmonically morphing Trailing Edge Flap (TEF). An unsteady parametrization method adapted for harmonic morphing is introduced, and then coupled with dynamic meshing to drive the morphing process. The turbulence characteristics are calculated using the hybrid Stress Blended Eddy Simulation (SBES) RANS-LES model. The far-field tonal noise is predicted using the Ffowcs-Williams and Hawkings (FW-H) acoustic analogy method with corrections to account for spanwise effects using a correlation length of half the airfoil chord. At various morphing frequencies and amplitudes, the 2D aeroacoustic tonal noise spectra are obtained for a NACA 0012 airfoil at a low angle of attack (AoA = 4°), a Reynolds number of 0.62 × 106, and a Mach number of 0.115, respectively, and the dominant tonal frequencies are predicted correctly. The aerodynamic coefficients of the un-morphed configuration show good agreement with published experimental and 3D LES data. For the harmonically morphing TEF case, results show that it is possible to achieve up to a 3% increase in aerodynamic efficiency (L/D). Furthermore, the morphing slightly shifts the predominant tonal peak to higher frequencies, possibly due to the morphing TEF causing a breakup of large-scale shed vortices into smaller, higher frequency turbulent eddies. It appears that larger morphing amplitudes induce higher sound pressure levels (SPLs), and that all the morphing cases induce the shift in the main tonal peak to a higher frequency, with a maximum 1.5 dB reduction in predicted SPL. The proposed dynamic meshing approach incorporating an SBES model provides a reasonable estimation of the NACA 0012 far-field tonal noise at an affordable computational cost. Thus, it can be used as an efficient numerical tool to predict the emitted far-field tonal noise from a morphing wing at the design stage.
Changes in Acidic Soil Chemical Properties and Carbon Dioxide Emission Due to Biochar and Lime Treatments
Authors: Mosharrof Mehnaz , Uddin Md. Kamal , Jusop Shamshuddin , Sulaiman Muhammad Firdaus , Shamsuzzaman S. M. , Haque Ahmad Numery Ashfaqul
Published: March 2021

To mitigate global climate change and simultaneously increase soil productivity, the use of biochar in agriculture can be a modern agro-technology that can help in reducing greenhouse gas emissions, enhancing soil carbon sequestration, and ultimately increasing crop yield. This study aimed to evaluate the effects of biochar and lime application on the chemical properties of acid soil and the emission of CO2. A 60-day incubation study was conducted with eleven treatments (T) in which two different biochar produced from rice husk (RHB) and oil palm empty fruit bunches (EFBB) at two rates (10 and 15 t ha−1) and on three rates of dolomitic limestone (100%, 75%, and 50%), recommended rate of NPK and a control (no amendment). The result showed that biochar and lime significantly increased soil pH, available P, and decreased exchangeable Al compared to the control. The pH increase was 44.02% compared to the control treatment on day 15, and the available P was found to be 22.44 mg kg−1 on day 30 from Treatment 7 (75% lime + 15 t ha−1 RHB). The cumulative CO2 emission from T7 was 207.40 μmol CO2 m−2 that decreased 139.41% compared to the control. Our findings conclude that RHB with 75% lime has more potential than EFBB to increase nutrient availability and reduce the emission of CO2 in acid soil.
Estimation of the Hourly Global Solar Irradiation on the Tilted and Oriented Plane of Photovoltaic Solar Panels Applied to Greenhouse Production
Authors: Diez Francisco J. , Martínez-Rodríguez Andrés , Navas-Gracia Luis M. , Chico-Santamarta Leticia , Correa-Guimaraes Adriana , Andara Renato
Published: March 2021

Agrometeorological stations have horizontal solar irradiation data available, but the design and simulation of photovoltaic (PV) systems require data about the solar panel (inclined and/or oriented). Greenhouses for agricultural production, outside the large protected production areas, are usually off-grid; thus, the solar irradiation variable on the panel plane is critical for an optimal PV design. Modeling of solar radiation components (beam, diffuse, and ground-reflected) is carried out by calculating the extraterrestrial solar radiation, solar height, angle of incidence, and diffuse solar radiation. In this study, the modeling was done using Simulink-MATLAB blocks to facilitate its application, using the day of the year, the time of day, and the hourly horizontal global solar irradiation as input variables. The rest of the parameters (i.e., inclination, orientation, solar constant, albedo, latitude, and longitude) were fixed in each block. The results obtained using anisotropic models of diffuse solar irradiation of the sky in the region of Castile and León (Spain) showed improvements over the results obtained with isotropic models. This work enables the precise estimation of solar irradiation on a solar panel flexibly, for particular places, and with the best models for each of the components of solar radiation.
Genotype by Environment Interaction Analysis of Agronomic Spring Barley Traits in Iceland Using AMMI, Factorial Regression Model and Linear Mixed Model
Authors: Hilmarsson Hrannar Smári , Rio Simon , Sánchez Julio Isidro y
Published: March 2021

Spring barley (Hordeum vulgare L.) is the most important cereal in Iceland and its national breeding program aims to select barley genotypes adapted to its environment. A critical step to understand the adaptation of Nordic barley material to a cool maritime climate is to assess the genotype by environment interaction (GxE). In this study, we evaluated the yield and thousand-kernel weight (TKW) of 32 spring barley genotypes in seven Icelandic environments. We applied three methods to analyze GxE: the additive main effects and multiplicative interaction model, a factorial model, and a linear mixed model. For yield, GxE was mainly caused by a better response of six-row genotypes compared to two-row genotypes in high fertility soils. For TKW, GxE showed a pattern along a gradient of daily mean temperatures. This pattern translated into a divergent TKW response between the 2-row and 6-row genotypes, with substantial crossovers along the temperature gradient. This GxE pattern was disentangled using all three methods, illustrating the value of cross-analysis. As yield is the main trait of interest for barley cultivation in Iceland, and few crossovers of genotype performance have been observed between environments, the definition of one mega-environment was recommended for Icelandic cultivation and breeding. We identified promising genetic material for both traits and highlighted the superiority of six-row genotypes for yield.
Sunflower Metabolites Involved in Resistance Mechanisms against Broomrape
Authors: Rial Carlos , Varela Rosa M. , Molinillo José M.G. , Peralta Sara , Macías Francisco A.
Published: March 2021

Several strategies have been proposed to control parasitic plants and these include biological control, the use of herbicides, and suicidal germination or trap crops. In the case of sunflower, the most effective solution is genetic resistance. Nevertheless, the use of resistant cultivars is followed by the appearance of new and more virulent races of the parasite that overcome the existing sources of resistance. For this reason, it is necessary to increase our knowledge of the mechanisms involved in the resistant varieties. In this study, the presence of compounds involved in pre-attachment resistance mechanisms in sunflower varieties was analyzed. The production of stimulators of the germination of parasitic plant seeds and the concentration of the haustorial elongation inhibitor, scopoletin, were measured in exudates of resistant and susceptible sunflower varieties. It was found that dehydrocostuslactone and orobanchyl acetate are not involved in the resistance of the sunflower varieties studied. The results demonstrate that costunolide, heliolactone, and scopoletin are related to sunflower resistance. The sunflower varieties studied do not use all available options to fight parasitic plants. This could be one of the reasons that sunflower resistance is rapidly overcome by broomrape.
Date Palm Pollen: Features, Production, Extraction and Pollination Methods
Authors: Salomón-Torres Ricardo , Krueger Robert , García-Vázquez Juan Pablo , Villa-Angulo Rafael , Villa-Angulo Carlos , Ortiz-Uribe Noé , Sol-Uribe Jesús Arturo , Samaniego-Sandoval Laura
Published: March 2021

Date palm pollen (DPP) plays a very important role in the fertilization process, since its viability and the pollination method influence on the quality, development, and yield of the fruit. In the present study, a broad review of its main characteristics, consumption, and DPP production are presented, as well as a description of its extraction methods and viability tests. The evolution of the pollination methods used in the date palm is also presented, from its natural pollination to the use of specialized mechanical and electrical devices, as well as the use of dry DPP and the current trend towards the use of DPP in liquid suspension. Likewise, the efficiency of the methods of natural pollination (wind); traditional (strands placement); dusting hand; dusting with manual, mechanical, or electric pollinator; and liquid pollination were evaluated from the fruit set percentage (FSP). Finally, starting from a scientometric analysis, the pollination methods were widely discussed, concluding that the dusting spraying of pollen suspension with liquid DPP is the pollination method that commonly presents the highest FSP, followed by dusting dry DPP with a motorized pollinator.
Issues of Feeding Strategy for Lactating Cows in Vietnamese Smallholder Dairy Farms
Authors: Bang Nguyen N. , Chanh Nguyen V. , Trach Nguyen X. , Khang Duong N. , Hayes Ben J. , Gaughan John B. , Lyons Russell E. , Hai Nguyen T. , McNeill David M.
Published: March 2021

A limited literature suggests relatively simple feeding regimes and diet formulation strategies for dairy cows in Vietnamese smallholder dairy farms (SDFs). This study aimed to classify and compare feeding regimes and nutrient balance for lactating cows between four typical dairy regions (south lowland, south highland, north lowland, and north highland) in Vietnam and evaluate the possibility of systematic dietary imbalance. Eight SDFs from each of the four regions were visited for two adjacent milking periods per farm. For each visit, frequency and methods of feed and water supply to the lactating cows were recorded, and individual fat corrected milk yield (ECM) of lactating cows were calculated from milk yield and fat concentration. The amount of each diet ingredient offered and refused by each lactating group was weighed and sampled for calculation of dry matter intake per cow (DMI) and analysis of nutrient composition in the component offered. PCDairy, a diet formulation computer model, was used to calculate actual and recommended dietary nutrient concentrations and predict potential milk production. Factor analysis, cluster analysis, and ANOVA were applied to determine grouping effects across as well as between regions. Feeding regimes and diets were grouped into three and nine clusters, respectively. Farmers in the same region tended to apply similar diets and feeding regimes. Across regions, only 47% of all SDFs supplied water ad libitum to the cows. The most used roughages including Napier grass, corn silage, fresh corn with cob, and rice straw were all relatively high in neutral detergent fibre (NDF), acid detergent fibre (ADF), and acid detergent lignin (ADL). The diets in all regions were excessive in crude protein, NDF, ADF, ADL, and most minerals (Ca, P, Mg, K, Na, S, Fe, Zn, Cu, and Mn) but insufficient in net energy and non-fibre carbohydrate. Feed efficiency (1.06 kg FCM/kg DMI) of the diets were sub-optimal. Feeding regimes and dietary nutrient balance of the south lowland SDFs were most problematic. Increasing dietary net energy concentration by increasing the use of starch and fat and decreasing dietary fibre concentration by decreasing the use of Napier grass or rice straw to balance the diets might help improve the milk production and thereby increase feed efficiency.
New Value to Wool: Innovative Garments for Preservation of Sheep Landraces in Italy
Authors: Sardaro Ruggiero , La Sala Piermichele
Published: March 2021

In Basilicata, southern Italy, a sheep landrace jeopardized of extinction is Gentile di Puglia due to low production levels, low market values of milk and meat, and replacement of wool with synthetic fibers. Due to these dynamics farmers progressively resort to intensive breeding systems, hence causing the gradual disappearance of the ovine sector, the withering of traditional breeding culture and the abandonment of internal and marginal territories. However, in changing climate, traditional agriculture is getting increased attention worldwide by the consumers who are embracing emerging sustainable food production. Thus, in the light of a possible conservation strategy, the study investigates the prospective market for a garment (pullover) produced with wool from Gentile di Puglia, and woven through traditional techniques. An integrated methodological approach based on choice experiments and Bass diffusion model was carried out in order to analyze the consumers’ preferences, the penetration market of this innovative product and the new wool value for farmers. The results pointed out a potential demand focused on women aged 50 years and more and a recognized wool value to farmers of 55 € animal−1 (22 € kg−1). This new revenue could allow the reduction of the difference in gross margin between the Gentile di Puglia and the non-autochthonous intensively-farmed Comisana from 57% to 3%. The production of further wool garments for a wider demand could increase the economic sustainability of Gentile di Puglia, making it even more preferable than other highly productive breeds.
Effects of Fouling Management and Net Coating Strategies on Reared Gilthead Sea Bream Juveniles
Authors: Comas Jordi , Parra David , Balasch Joan Carles , Tort Lluís
Published: March 2021

In aquaculture, biofouling management is a difficult and expensive issue. Cuprous oxide has been commonly used to prevent fouling formation. To cheapen net management and reduce the use of copper, the industry has proposed several alternatives. Currently, polyurethane coatings are being explored and commercially implemented. With this alternative, net cleaning is done in situ, reducing the number of nets necessary to raise a batch, thus ideally reducing operational costs. This pilot study compared this new strategy to the use of cuprous oxide. The results show that nets treated with antifouling perform better and bioaccumulation of copper in fish tissues do not pose health risks to fish. Alternatives involving on-site cleaning need to improve efficiency. Although the conditions of this work are not completely comparable to commercial aquaculture conditions, the results might indicate the strengths and constrains of the solutions tested in real life.
Effects of Diet and Phytogenic Inclusion on the Antioxidant Capacity of the Broiler Chicken Gut
Authors: Griela Eirini , Paraskeuas Vasileios , Mountzouris Konstantinos C.
Published: March 2021

The reduction in energy and protein dietary levels, whilst preserving the gut health of broilers, is warranted in modern poultry production. Phytogenic feed additives (PFAs) are purported to enhance performance and antioxidant capacity in broilers. However, few studies have assessed PFA effects on a molecular level related to antioxidant response. The aim of this study was to investigate the effects of administering two dietary types differing in energy and protein levels (L: 95% and H: 100% of hybrid optimal recommendations) supplemented with or without PFA (−, +) on gene expressions relevant for antioxidant response along the broiler gut. Interactions of diet type with PFA (i.e., treatments L−, L+, H−, H+) were determined for critical antioxidant and cyto-protective genes (i.e., nuclear factor erythroid 2-like 2 (Nrf2) pathway) and for the total antioxidant capacity (TAC) in the proximal gut. In particular, the overall antioxidant response along the broiler gut was increased upon reduced dietary energy and protein intake (diet type L) and consistently up-regulated by PFA addition. The study results provide a new mechanistic insight of diet and PFA functions with respect to the overall broiler gut antioxidant capacity.
Using PRRSV-Resilient Sows Improve Performance in Endemic Infected Farms with Recurrent Outbreaks
Authors: Abella Gloria , Pagès-Bernaus Adela , Estany Joan , Pena Ramona Natacha , Fraile Lorenzo , Plà-Aragonés Lluis Miquel
Published: March 2021

The selection of porcine reproductive and respiratory syndrome (PRRS) resilient sows has been proposed as a strategy to control this disease. A discrete event-based simulation model was developed to mimic the outcome of farms with resilient or susceptible sows suffering recurrent PRRSV outbreaks. Records of both phenotypes were registered in a PRRSV-positive farm of 1500 sows during three years. The information was split in the whole period of observation to include a PRRSV outbreak that lasted 24 weeks (endemic/epidemic or En/Ep) or only the endemic phase (En). Twenty simulations were modeled for each farm: Resilient/En, Resilient/En_Ep, Susceptible/En, and Susceptible/En_Ep during twelve years and analyzed for the productive performance and economic outcome, using reference values. The reproductive parameters were generally better for resilient than for susceptible sows in the PRRSV En/Ep scenario, and the contrary was observed in the endemic case. The piglet production cost was always lower for resilient than for susceptible sows but showed only significant differences in the PRRSV En/Ep scenario. Finally, the annual gross margin by sow is significantly better for resilient than for susceptible sows for the PRRSV endemic (12%) and endemic/epidemic scenarios (17%). Thus, the selection of PRRSV resilient sows is a profitable approach for producers to improve disease control.
Effect of Citric Acid on Color Changes of Calcium Silicate-Based Cements an In Vitro Study
Authors: Metlerska Joanna , Dammaschke Till , Lipski Mariusz , Fagogeni Irini , Machoy-Mokrzyńska Anna , Nowicka Alicja
Published: March 2021

The aim of the present in vitro study was to investigate the effects of 10% and 40% citric acid (CA) on the color of calcium silicate–based cements (CSCs) in comparison to the effects of common root canal irrigants. Samples of six CSCs (n = 6)—ProRoot MTA (Dentsply, Tulsa, OK, USA), Biodentine (Septodont, Saint-Maur-des-Fossés, France), MTA Plus (Avalon Biomed Inc, by Prevest Denpro Limited, Jammu, India), MTA Repair HP (Angelus, Londrina, PR, Brazil), Ortho MTA (BioMTA, Seoul, Korea), and Retro MTA (BioMTA, Seoul, Korea)—were immersed in 10% and 40% CA as well as 15% EDTA, 2% NaOCl, 2% CHX, and 0.9% NaCl for 15 min, 1 h, and 24 h. ΔE values, representing the difference between the final and baseline values of the color components, were then determined using a VITA Easyshade Compact 5.0 spectrophotometer. Naked-eye evaluation of the changes in color and structures of the materials was performed using our own scale. Upon immersion of the materials in both 10% and 40% CA, there were statistically significant differences between spectrophotometric color measurement results for all CSCs (P < 0.05). However, CA does not cause dark discoloration, observable with the naked eye, of any of the materials, such as NaOCl and CHX. Significant statistical differences were also found between all CSCs in terms of submersion duration (P < 0.05). CA, which could be an alternative to EDTA use, caused greater CSCs discoloration and changed some of their structures. Unless required by the therapeutic procedure, clinicians should pay attention to the fact that the irrigant may affect the CSCs discoloration and minimize the contact time of irrigant with CSCs.
Numerical Analysis of the Load-Displacement Behaviour of Cast-in-Place Progressive Anchorage in Reinforced Concrete Members
Authors: Farbák Matúš , Jošt Jozef , Hlinka Richard , Rosmanit Miroslav
Published: March 2021

Modern construction requirements for building structures are currently focused on reducing the time required for construction, dealing with the lack of qualified human resources and ensuring comprehensive construction work quality. The problems mentioned above of today’s construction industry are significantly reduced by modern prefabrication and the efficient use of the most common building materials—steel and concrete. Critical components of such construction systems are their joints. Currently, there are many different types of joints of precast concrete structural elements. Integral parts of these joints are the various anchorages. For connecting load-bearing components, cast-in-place anchor systems are preferred to post-installed ones. The appropriate design of this small but crucial structural component is a complicated engineering issue in some cases. The finite element method (FEM) represents a practical opportunity to design and analyze anchorage systems in detail. A detailed numerical study based on an experimental program was performed to understand cast-in-place anchors’ real behavior and clarify some of the parameters of their design. This paper explains the creation of a numerical model, compares the FEM model with the performed experiments and presents the interesting results of the performed parametric study.
Coordinating Entrainment Phenomena: Robot Conversation Strategy for Object Recognition
Authors: Kimoto Mitsuhiko , Iio Takamasa , Shiomi Masahiro , Shimohara Katsunori
Published: March 2021

This study proposes a robot conversation strategy involving speech and gestures to improve a robot’s indicated object recognition, i.e., the recognition of an object indicated by a human. Research conducted to improve the performance of indicated object recognition is divided into two main approaches: development and interactive. The development approach addresses the development of new devices or algorithms. Through human–robot interaction, the interactive approach improves the performance by decreasing the variability and the ambiguity of the references. Inspired by the findings of entrainment and entrainment inhibition, this study proposes a robot conversation strategy that utilizes the interactive approach. While entrainment is a phenomenon in which people unconsciously tend to mimic words and/or gestures of their interlocutor, entrainment inhibition is the opposite phenomenon in which people decrease the amount of information contained in their words and gestures when their interlocutor provides excess information. Based on these phenomena, we designed a robot conversation strategy that elicits clear references. We experimentally compared this strategy with the other interactive strategy in which a robot explicitly requests clarifications when a human refers to an object. We obtained the following findings: (1) The proposed strategy clarifies human references and improves indicated object recognition performance, and (2) the proposed strategy forms better impressions than the other interactive strategy that explicitly requests clarifications when people refer to objects.
The Impact of Force Factors on the Benefits of Digital Transformation in Romania
Authors: Căpușneanu Sorinel , Mateș Dorel , Tűrkeș Mirela Cătălina , Barbu Cristian-Marian , Staraș Adela-Ioana , Topor Dan Ioan , Stoenică Laurențiu , Fűlöp Melinda Timea
Published: March 2021

The digital transformation has produced changes in all existing areas of activity worldwide. There are many factors that can influence the intention to use Industry 4.0 processes and solutions and change the behavior of organizations and their business models. The aim of this study is to validate the econometric model on assessing the significant impact of distinct factors on the intention to use Industry 4.0 processes and solutions, the benefits of digital transformation perceived by organizational management and the differences between distinct groups analyzed. The research method used within the quantitative study was the sample survey, using the online questionnaire as a data collection tool. Three hundred forty-seven valid questionnaires were collected and the response rate of the respondents was 64.25%. A new structural model was generated based on the elements of the Unified Theory of Acceptance and Use of Technology (UTAUT). The results of the study indicated that Perceived competitiveness and Perceived risk have a significant impact on Intention to Use Industry 4.0 processes while Perceived vertical networking solutions and Perceived integrated engineering solutions have a significant influence on the Intention to Use Industry 4.0 solutions. In conclusion, there is a positive and significant association between Intention to Use Industry 4.0 solutions and Benefits of Digital Transformation.