bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Cultivar, Processing and Storage on the Mycobiota of European Chestnut Fruits
Authors: Rodrigues Paula , Driss Jihen Oueslati , Gomes-Laranjo José , Sampaio Ana
Published: November 2022

Sweet chestnut fruits are popular fruits commercialized as fresh or processed ready-to-eat products. The major post-harvest problems associated with stored chestnut fruits are fungal rots, which cause major losses in fruit quality. The aims of this work were to determine the incidence, abundance and diversity of rots and fungi in three chestnut varieties (Longal, Judia and Martaínha) of Portugal, collected from an industrial plant, and to identify the stages of storage and processing where fungi and rots are more significant. Thirty-three chestnut samples from the three varieties were collected from different stages of industrial processing. Nuts were internally and externally inspected for damage, infestation and infection, and internal fungi were isolated and molecularly identified. The variety Martaínha was identified as the least susceptible to fungal growth, while Longal was the most susceptible. A high diversity of fungi was detected and identified. The dominant fungi were Mucor racemosus, Penicillium spp. (the causal agents of green rots), Ciboria batschiana (black rot) and Botrytis cinerea (gray rot). Gnomoniopsis smithogilvyi, the causal agent of brown rot, was also frequently detected. Sterilization with hydrothermal bath was effective in the elimination or reduction of most of the rot-causing fungi. These results could serve as a baseline for better monitoring fungal development and chestnut decay, and to develop effective management measures to control post-harvest chestnut rots.
Workability Assessment of Different Stony Soils by Soil–Planter Interface Noise and Acceleration Measurement
Authors: Toscano Pietro , Cutini Maurizio , Filisetti Alex , Premoli Elia , Porcu Maurizio , Catalano Nicola , Bisaglia Carlo , Brambilla Massimo
Published: November 2022

Sowing is critical for successful crop establishment and productivity, particularly in precision agriculture management strategies. However, topsoil characteristics directly affect agribusiness maximization (i.e., crop-yield increase, machinery efficiency, operating-cost reduction) even in the most advanced farming management techniques. The excessive presence of coarse fractions or stones in arable soil layers prevents modern machinery from reaching optimal efficiency. This work focuses on sowing to verify whether the vibration and noise arising during this operation significantly change with varying soil conditions according to the stoniness degree of disturbance on soil workability. To make this assessment, an experimental sowing activity was carried out on four soil plots with two different disturbance degrees. The results confirmed that the noise and acceleration of the sowing machine significantly correlated with the soil disturbance degree and related workability profile.
The Effect of High-Pressure Pre-Soaking on the Water Absorption, Gelatinization Properties, and Microstructural Properties of Wheat Grains
Authors: Shorstkii Ivan , Sosnin Maxim , Mounassar Emad Hussein Ali , Bindrich Ute , Heinz Volker , Aganovic Kemal
Published: November 2022

High-pressure processing (HPP) is a novel technology that is used in many food processing operations to increase both the efficiency and a reduction in the energy and time required to modify and improve the physical and chemical properties of traditional food products. The objective of this study was to investigate the impacts of applying three treatments of a high hydrostatic pressure (HHP) and a control, i.e., 0, 100, 300 and 600 MPa, on the water absorption, gelatinization properties, and microstructural changes of wheat grains. The results indicated that the HHP treatments with a pressure of 300 and 600 MPa resulted in an increase of 16.7–24.8% in the mass of the grains; however, the pressure of 600 MPa did not result in a mass increase through water uptake. Further, the transition enthalpy increased with the HHP pressure, with 600 MPa defined as the threshold value for pressure. The results from this study demonstrated that a HHP treatment may enhance the soaking process of wheat grains and, thus, positively affect their gelatinization properties. These preliminary results may be used to improve the processing efficiency and quality of wheat-based products.
Effects on Lettuce Yield Parameters and Toxicological Safety Assessment of a Plant-Derived Formulation Based on Rosemary and Eucalyptus Essential Oils
Authors: Kapnisis Konstantinos , Chrysargyris Antonios , Prokopi Marianna , Varda Eleni , Kokkinidou Despoina , Samourides Andreas , Xylia Panayiota , Onisiforou Pavlina , Stavrinides Menelaos , Tzortzakis Nikolaos , Anayiotos Andreas
Published: November 2022

Essential oils from medicinal and aromatic plants are increasingly recognized as a promising class of green molecules for use in crop production. In many cases, the beneficial aspects of a substance are not supported by sufficient toxicological safety testing, even though recent reports suggest that some compounds may be toxic to terrestrial or aquatic non-target species. It is, therefore, essential to investigate the possibility of adverse effects on non-target animals and humans exposed to these substances through the consumption of fruit and/or vegetables. The present study aims to examine the potential effects on yield and quality parameters and investigate the level of in vitro and in vivo toxicity of an Eco-product (EP) based on rosemary and eucalyptus essential oils, to provide a measure for safe use in the agricultural sector. The product was evaluated in lettuce crop production and indicated that one-time application of the EP formula increases yield, activating various secondary metabolism pathways of the plant to cope with oxidative stress. Cytotoxicity assays and in vivo acute oral and dermal toxicity studies suggest that the tested compound does not pose any significant health hazard, and the dissolved product can be classified in Category 5, according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).
Location of Fruits by Counting: A Point-to-Point Approach
Authors: Li Bo , Chen Cheng
Published: November 2022

The emergence of deep learning-based methods for harvesting and yield estimates, including object detection or image segmentation-based methods, has notably improved performance but has also resulted in large annotation workloads. Considering the difficulty of such annotation, a method for locating fruit is developed in this study using only center-point labeling information. To address point labeling, the weighted Hausdorff distance is chosen as the loss function of the corresponding network, while deep layer aggregation (DLA) is used to contend with the variability in the visible area of the fruit. The performance of our method in terms of both detection and position is not inferior to the method based on Mask-RCNN. Experiments on a public apple dataset are provided to further demonstrate the performance of the proposed method. Specifically, no more than two targets had positioning deviations exceeding five pixels within the field of view.
Smallholders’ Water Management Decisions in the Face of Water Scarcity from a Socio-Cognitive Perspective, Case Study of Viticulture in Mendoza
Authors: Monnet Marc , Vignola Raffaele , Aliotta Yoana
Published: November 2022

Grape producers in the Province of Mendoza (Argentina) are extremely vulnerable to the current water crisis, especially smallholders who have very limited resources to adapt. The discourse on adaptation options is mainly technocratic with a focus on modern irrigation systems not accessible to the majority of grape producers. Thus, this research aims at shedding light and providing information for the design of inclusive adaptation strategies by identifying, with a socio-cognitive model, feasible adaptation options according to grape producers’ perceptions and the related implementation barriers. Grape producers’ water scarcity and adaptation appraisal were explored through qualitative interviews in the Northern Oasis (Mendoza) to better understand how producers’ intentions are shaped through perceptual and socio-cognitive processes. To do so, a socio-cognitive model on grape producers’ adaptation to water scarcity (GPAWS) was developed based on two similar models. The analysis reveals that, as overall grape producers share a similar concern with the risk of water scarcity, their different adaptive behaviours tend to be mostly derived from their differences in adaptation appraisal. Moreover, producers’ adaptation intentions are mainly reactive and limited to answer short term, immediate risks. Most of the grape producers perceive feasibility and plan the implementation of reasonable efficiency measures. However, multiple barriers consequently limit the implementation of such adaptation options perceived as feasible by the producers. The results of this research can support government actors, agriculture research institutes, but also the cooperatives of producers seeking to encourage farmers’ adaptation, by identifying which adaptation options could be implemented according to the type of producers and their adaptation appraisal, but also why certain feasible measures are not being implemented.
Effect of Nitrogen Nutrition and Planting Date on the Yield and Physicochemical Parameters of Flowering Chinese Cabbage
Authors: Liu Wenping , Muzolf-Panek Małgorzata , Kleiber Tomasz
Published: November 2022

The nitrogen requirements of flowering Chinese cabbage are unknown. Our study aimed at investigating the effect of varied nitrogen nutrition levels (including 50, 70, 90, 110, and 130 mg N per dm3, described as N-50 to N-130, respectively) on the yield of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) grown in two varied soilless cultivation systems (substrate and hydroponic) and seasons (spring and autumn). We confirmed that the intensity of the nitrogen nutrition modified the yield of plants within a range of 50 to 90 mg N; the yields were increased; however, the higher N concentrations were not effective. In both cultivation systems, the content of K, Ca, and Fe in leaves was higher in autumn compared to spring. Nitrogen nutrition improved the weight of plants—an effect that varied depending on the system of cultivation—and increased the phenolic content. N-90 was the optimal level of nitrogen nutrition. More intensive N-nutrition did not significantly modify plant yields or phenolic content. We Concluded that N fertilisation might be an effective tool to obtain plants with high bioactive compound content.
Changes in Sucrose and Sorbitol Metabolism Cause Differences in the Intrinsic Quality of Peach Fruits Cultivated in Field and Greenhouse Environments
Authors: Xu Gongxun , Li Chen , Qin Sijun , Xiao Wei , Fu Xiling , Chen Xiude , Li Ling , Li Dongmei
Published: November 2022

Fruit trees grow in complex environments where various environmental factors are related to each other, exerting a comprehensive effect on fruit quality. In this study, diurnal variations in environmental indices in the field and greenhouse were recorded, and the changes of leaf photosynthetic assimilate metabolism and fruit soluble sugar accumulation in peach (Prunus persica) under the influence of a comprehensive environment were explored. The results showed that the field environment was more favorable for peach photosynthesis, and more sucrose, glucose and fructose could be accumulated compared with the greenhouse environment. In addition, more sorbitol was converted into glucose and fructose in field fruits. Therefore, field fruits exhibited a particularly greater increase in the fructose content, which greatly increased the sweetness of field fruits. This study revealed changes in the pattern of sucrose and sorbitol metabolism in peaches grown in the field and greenhouse, and analyzed the possible reasons and mechanisms of fruit intrinsic quality differences. This research will provide a theoretical basis and reference for the regulation of fruit quality in the greenhouse environment.
Characterization and Identification of Native Pseudomonads from Red and Lateritic Regions of West Bengal
Authors: Kumar Ritesh , Dutta Subrata , Roy Barman Ashis , Sen Krishnendu , Datta Gauranga , Ghorai Ankit Kumar , Bharati Desh Raj Shri , Kumar Anshu , Das Raju , Ray Sujit Kumar
Published: November 2022

Agricultural crops are facing a continuous threat due to biotic and abiotic stresses, thus, limiting the crop productivity, and thereby, threatening food security. Plant roots attract several kinds of microbes that induce resistance in plants against these stresses by enhancing the activity of the antioxidant enzymes, phenolic and other non-phenolic compounds, and thereby, have a beneficial effect on plants. Vast research has been carried out on biocontrol agents to manage soil-borne plant pathogens, but there has been limited success in the development of region-specific, commercially viable microbial inoculants. The present research was framed with a view to screen and evaluate native Pseudomonads from the rhizosphere of different crops in lateritic soils and their exploitation in biotic and abiotic stress management under the red and lateritic zone of West Bengal. In the lateritic area of West Bengal, the lowest pH as well as the highest culturable rhizobacterial population was found in the soil of Bankura. Among all the isolated rhizobacteria, 43.33% were found to be moderately antagonistic against three different soil-borne plant pathogens viz., Rhizoctonia solani, Sclerotinia sclerotiorum and Sclerotium rolfsii—while only 6.67% were found to be very highly antagonistic against these soil-borne plant pathogens. Augmented seeds of tomato, cowpea and French bean with native rhizobacteria enhanced the vigour index and 16.67% of the isolates were found to have a high value of vigour index in the normal and acid stress conditions. Based on acid tolerance, antagonistic activity and the seed vigour assay, a total of 13 isolates from 97 of lateritic area were selected. Out of the 13 selected isolates, 7 were positive for protease and lipase production, 8 isolates were positive for the production of HCN, siderophore and salicylic acid production and 9 isolates were found to be positive for IAA, phosphate solubilisation, amylase and chitinase production. Species of beneficial Pseudomonads such as Pseudomonas aeruginosa, P. fluorescens, P. Plecoglossicida, P. helmanticensis, P. geniculate, P. baetica and P. putida were found. Five isolates were used to study the effect on plant growth in terms of germination (%), root and shoot length, as well as fresh root and shoot weight and disease patterns in terms of pre- and post-emergence damping-off under the semi-field condition.
Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms
Authors: Suzuki Kasumi , Shinkai Hiroki , Yoshioka Gou , Matsumoto Toshimi , Takenouchi Takato , Tanaka Junji , Shimizu Masanori , Kitazawa Haruki , Uenishi Hirohide
Published: November 2022

Reduced productivity caused by infections, particularly respiratory diseases, is a serious problem in pig farming. We have previously reported polymorphisms in porcine pattern recognition receptor genes affecting molecular functions and demonstrated that the 2197A/C polymorphism in the nucleotide-binding oligomerization domain containing 2 (NOD2) gene influences porcine circovirus 2-induced mortality. Here, we investigated how these polymorphisms affect respiratory disease-induced lesions, using samples from a slaughterhouse dealing with pigs from two farms. Lung lesions were evaluated using two scoring systems, Goodwin (GW) and slaughterhouse pleuritis evaluation system (SPES), to determine the influence of Mycoplasma hyopneumoniae (Mhp) and Actinobacillus pleuropneumoniae (App), respectively. SPES scores were significantly higher when the 1205T allele of Toll-like receptor 5 (TLR5-1205T), rather than TLR5-1205C, was present. On the farm with more severe Mhp invasion, lower GW lesion scores were significantly associated with the presence of the NOD-like receptor family pyrin domain containing 3 (NLRP3)-2906G allele; where App invasion was worse, lower SPES scores were significantly associated with the presence of the NOD2-2197C allele. Combinations of polymorphisms in pattern recognition receptor genes can therefore be utilized for breeding for resistance against respiratory diseases in pigs. DNA markers of these polymorphisms can thus be used to improve productivity by reducing respiratory diseases due to bacterial pathogens in pig livestock.
Assessing Molecular Diversity in Native and Introduced Populations of Red Wood Ant Formica paralugubris
Authors: Masoni Alberto , Coppi Andrea , Balzani Paride , Frizzi Filippo , Fani Renato , Zaccaroni Marco , Santini Giacomo
Published: November 2022

The Formica rufa group comprises several ant species which are collectively referred to as “red wood ants” and play key roles in boreal forest ecosystems, where they are ecologically dominant and greatly influence habitat dynamics. Owing to their intense predatory activity, some of these species are used as biocontrol agents against several forest insect pests and for this aim in Italy, nearly 6000 ant nests were introduced from their native areas in the Alps to several Appeninic sites during the last century. In this work, we assessed and compared the genetic variability and structure of native and introduced populations of F. paralugubris, thus evaluating the extent of genetic drift that may have occurred since the time of introduction, using amplified fragment length polymorphism (AFLP) markers. PCR amplification with a fam_EcoRI-TAC/MseI-ATG primers combination produced a total of 147 scorable bands, with 17 identified as outlier loci. The genetic variation was higher in the introduced population compared to the native ones that, on the other hand, showed a higher diversity between nests. AMOVA results clearly pointed out that the overall genetic structure was dominated by among-worker variation, considering all populations, the Alpine vs. Apennine groups and the comparison among native and related introduced populations (all ranging between 77.84% and 79.84%). Genetic analyses unveiled the existence of six main different groups that do not entirely mirror their geographic subdivision, pointing towards a wide admixture between populations, but, at the same time, rapid diversification of some Apennine populations. Future studies based on high-throughput genomic methods are needed to obtain a thorough understanding of the effects of environmental pressure on the genetic structure and mating system of these populations.
Cross Effects of Diets and Rearing Temperatures on Gastrointestinal Evacuation and Growth Performance in Adult Sabah Groupers (Epinephelus fuscoguttatus × E. lanceolatus)
Authors: Das Simon Kumar , De Moumita , Noor Noorashikin Md , Bakar Yosni , Cob Zaidi Che , Ghaffar Mazlan Abd.
Published: November 2022

This study explores the gastrointestinal evacuation time (GET) and gastrointestinal evacuation rate (GER) of the popular Sabah grouper (Epinephelus fuscoguttatus × E. lanceolatus) adults using two established methods (X-radiography and serial slaughter) and square root modeling using different temperatures: 28 °C, 30 °C, 32 °C, and 34 °C and different diets: pellet (ash: 11.4 ± 0.08; moisture: 29.0 ± 0.01; protein 37.5 ± 0.80; lipid 15.0 ± 0.13) and trash fish: Sardinella sp. (ash: 2.3 ± 0.15; moisture: 78.5 ± 0.33; protein 55.4 ± 0.62; lipid 7.3 ± 0.25) and the impact on growth indices. The results indicate that the GET shortened as temperature increased from 28 °C to 30 °C; however, it was prolonged when it surged to 32 °C and 34 °C. The groupers fed with trash fish at a temperature of 30 °C had the shortest GER (0.41 ± 0.10 g hr−1) whereas groupers fed with pellet at 34 °C had the longest GER (0.95 ± 0.02 g hr−1). Likewise, the highest SGR (16.25 ± 2.11% day−1) was observed at 30 °C for groupers fed with a trash fish diet. The condition (K) value was lowest at 34 °C for groupers fed with a pellet diet (1.01 ± 0.04) and highest at 30 °C for groupers fed with trash fish (1.45 ± 0.04). Our results suggest that temperature and diet influence growth indices and GE of adult Sabah groupers. Incorporation of this information will allow better management of this commercially important grouper species when reared in a controlled aquaculture environment.
Use of Olive Pulp for Gestating Iberian Sow Feeding: Influence on Performance, Health Status Indicators, and Fecal Microbiota
Authors: Sánchez Cristian Jesús , Barrero-Domínguez Belén , Martínez-Miró Silvia , Madrid Josefa , Baños Alberto , Aguinaga María Arántzazu , López Silvia , Hernández Fuensanta
Published: November 2022

Olive pulp (Olea europaea) inclusion in the diet of Iberian sows (Sus scrofa) is interesting due to fiber and bioactive compounds content and because both productions are located in the same area. The aim of this trial was to study the effect of olive pulp inclusion at 100 g/kg in Iberian sow’s diet on performance, immunoglobulin and serum parameters, antioxidant status, and fecal microbiota. Forty multiparous Iberian sows (body weight (BW) = 149.6 ± 20.2 kg) were assigned either a control diet (CON) or an experimental diet (PUL) with olive pulp at 100 g/kg. The BW and backfat thickness in sows were measured at post-insemination days 42 and 107, and litter performance was measured on the farrowing day. Blood and fecal samples were collected at gestation day 107. In piglets, blood was sampled when they were 10 days old for immunoglobulin analysis. Albumin, total protein, triglyceride, creatinine, urea, glucose, and Trolox equivalent antioxidant capacity in serum were higher (p < 0.05) in PUL sows than in CON sows. The Enterobacteriaceae, Bifidobacterium spp., and Lactobacillus spp. fecal counts were increased (p < 0.05) with olive pulp supplementation compared with the CON sow group. Olive pulp added to gestating Iberian sow’s diet at 100 g/kg has beneficial effects on the fecal microbiota and antioxidant status, without penalizing other gestation parameters.
A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes
Authors: Getino María , López-Díaz María , Ellaby Nicholas , Clark John , Ellington Matthew J. , La Ragione Roberto M.
Published: November 2022

IncL/M broad-host-range conjugative plasmids are involved in the global spread of blaOXA-48 and the emergence of blaNDM-1. The aim of this study was to evaluate the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The conjugation rate and fitness cost of IncM2 and IncL plasmids encoding these carbapenemase genes were tested using a variety of host bacteria. Genomic analysis of uropathogenic Escherichia coli SAP1756 revealed that blaNDM-1 was encoded on an IncM2 plasmid, which also harboured blaTEM-1, bleMBL and sul1 and was highly similar to plasmids isolated from the same geographical area. Conjugation experiments demonstrated that NDM-1 and OXA-48-carrying plasmids transfer successfully between different Enterobacterales species, both in vitro and in vivo. Interestingly, E. coli isolates tested as recipients belonging to phylogroups A, B1, D and F were able to receive IncM2 plasmid pSAP1756, while phylogroups B2, C, E and G were not permissive to its acquisition. In general, the IncL OXA-48-carrying plasmids tested transferred at higher rates than IncM2 harbouring NDM-1 and imposed a lower burden to their host, possibly due to the inactivation of the tir fertility inhibition gene and reflecting their worldwide dissemination. IncM2 plasmids carrying blaNDM-1 are considered emergent threats that need continuous monitoring. In addition to sequencing efforts, phenotypic analysis of conjugation rates and fitness cost are effective methods for estimating the pandemic potential of antimicrobial resistance plasmids.
Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii
Authors: Liu Mingyang , Zheng Xiaochuan , Sun Cunxin , Zhou Qunlan , Liu Bo , Xu Pao
Published: November 2022

Both oxidative stress and autophagy refer to regulating fat metabolism, and the former affects autophagy, but the role and mechanism of the antioxidant–autophagy axis in regulating lipid metabolism remains unclear. As an antioxidant, tea tree oil (TTO) has little research on the regulatory mechanism of lipid metabolism in crustaceans. This study investigated whether TTO could alter hepatopancreatic lipid metabolism by affecting the antioxidant–autophagy axis. Feed Macrobrachium rosenbergii with three different levels of TTO diets for 8 weeks: CT (0 mg/kg TTO), 100TTO (100 mg/kg TTO), and 1000TTO (1000 mg/kg TTO). The results showed that 100TTO treatment reduced the hemolymph lipids level and hepatopancreatic lipid deposition compared to CT. In contrast, 1000TTO treatment increased hepatopancreatic lipid deposition, damaging both morphology and function in the hepatopancreas. The 100TTO treatment promoted lipolysis and reduced liposynthesis at the transcriptional level compared to the CT group. Meanwhile, it improved the hepatopancreas antioxidant capacity and maintained mitochondrial structural and ROS homeostasis. In addition, it simultaneously activated the expression of transcription factors Keap1-Nrf2 and Imd-Relish. By contrast, the 1000TTO group significantly enhanced the ROS level, which considerably activated the Keap1-Nrf2 signaling expression but had no significant effects on the expression of Imd-Relish. The 100TTO group supplementation significantly enhanced lipid droplet breakdown and autophagy-related genes and protein expression. On the contrary, the 1000TTO group significantly inhibited the expression of genes and proteins related to autophagy. Pearson analysis revealed that Nrf2 has a positive correlation to lipid anabolism-related genes (Fasn, Srebp1, Pparγ) and autophagy regulators (mtor, akt, p62), and were negatively correlated with lipolysis-related genes (Cpt1, Hsl, Ampkα) and autophagy markers (Ulk1, Lc3). Relish was positively correlated with Atgl, Cpt1, Ampkα, Ulk1, and Lc3, and negatively correlated with Pparγ and p62. Moreover, Keap1 and Imd were negatively correlated with p62 and mtor, respectively. In sum, 100 mg/kg TTO enhanced antioxidant activity and increased autophagy intensity through the Relish-Imd pathway to enhance lipid droplet breakdown, while 1000 mg/kg TTO overexpressed Nrf2, thus inhibiting autophagy and ultimately causing excessive lipid deposition and peroxidation. Our study gives a fresh perspective for deciphering the bidirectional regulation mechanism of lipid metabolism by different doses of TTO based on the antioxidant–autophagy axis.
Hedgehog Signalling Contributes to Trauma-Induced Tendon Heterotopic Ossification and Regulates Osteogenesis through Antioxidant Pathway in Tendon-Derived Stem Cells
Authors: Li Guanzhi , Deng Ye , Li Kaiqun , Liu Yuchen , Wang Ling , Wu Zhiyong , Chen Chao , Zhang Kairui , Yu Bin
Published: November 2022

Heterotopic ossification (HO) is defined as the generation of pathological ectopic bony structures in soft tissues, but the molecular mechanisms of tendon HO are not fully revealed. Hedgehog (Hh) signalling is reportedly critical in hereditary HO. Our study focuses on the role of Hh signalling in the formation of trauma-induced tendon ossification. In this study, samples of healthy tendons and injured tendons from C57BL/6J female mice at 1, 4, 7, and 10 weeks after Achilles tenotomy were collected for quantitative real-time polymerase chain reaction (qRT–PCR) and immunohistochemical analysis (IHC). At 1, 4, 7, and 10 weeks postinjury, tendon samples from the mice administered with vehicle, GANT58 (a GLI antagonist), or SAG (a smoothened agonist) were harvested for micro-CT, histological staining, qRT–PCR, and IHC. Rat tendon-derived stem cells (TDSCs) treated with vehicle, GANT58, or SAG were used to induce osteogenic and chondrogenic differentiation in vitro for qRT–PCR, alkaline phosphatase staining, Alcian blue staining, and reactive oxygen species (ROS) levels measurement. We found that Hh signalling is remarkably activated during the formation of trauma-induced tendon ossification in the model of Achilles tenotomy. The in vitro and in vivo assays both confirm that downregulation of Hh signalling significantly suppresses osteogenesis and chondrogenesis to inhibit tendon ossification, while upregulation of Hh signalling promotes this process. Under osteogenic induction, Hh signalling regulates antioxidant pathway and affects ROS generation of TDSCs. Collectively, Hh signalling contributes to trauma-induced tendon ossification and affects ROS generation through antioxidant pathway in osteogenic differentiation of TDSCs, indicating that targeting Hh signalling by GANT58 may be a potential treatment for trauma-induced tendon ossification.
Analysis of Physiological Indicators Associated with Drought Tolerance in Wheat under Drought and Re-Watering Conditions
Authors: Wang Jiarui , Zhang Xiaoyan , Han Zhidong , Feng Haoxiang , Wang Yangyang , Kang Juan , Han Xiaojie , Wang Lifang , Wang Chenyang , Li Hua , Ma Geng
Published: November 2022

Wheat (Triticum aestivum L.) production is severely threatened by an increase in the frequency of drought events. It is crucial to determine stable and effective morphological, physiological, and associated oxidative stress indicators, to evaluate the drought tolerance of wheat for breeding and cultivation. Therefore, the cultivars Luohan 22 (LH 22, drought−tolerant) and Zhengmai 366 (ZM 366, drought−sensitive) were used as experimental materials to analyze the changes in 12 physiological and biochemical indicators, as well as the yield, when the stress was prolonged to different times. Re-watering after 6 days of drought can effectively alleviate the associated oxidative stress of drought to wheat. The physiological responses of plants were reversible when they were re-watered in the range of 6 to 12 days after drought. The degree of recovery of LH 22 was higher than that of ZM 366. Afterwards, seven indicators, including stomatal conductance, proline, malondialdehyde, soluble sugar, hexokinase, glucose, and the non−photochemical quenching parameter, were screened out to characterize tolerance of wheat to drought using the multivariate statistical analytical method. This study further investigated the method of evaluating and indexing tolerance of wheat to drought, from the physiological and biochemical levels. This study can provide a theoretical basis and reference for the selection of wheat cultivars to breed and cultivate against drought stress.
Lutein Decreases Inflammation and Oxidative Stress and Prevents Iron Accumulation and Lipid Peroxidation at Glutamate-Induced Neurotoxicity
Authors: Pap Ramóna , Pandur Edina , Jánosa Gergely , Sipos Katalin , Nagy Tamás , Agócs Attila , Deli József
Published: November 2022

The xanthophyll carotenoid lutein has been widely used as supplementation due to its protective effects in light-induced oxidative stress. Its antioxidant and anti-inflammatory features suggest that it has a neuroprotective role as well. Glutamate is a major excitatory neurotransmitter in the central nervous system (CNS), which plays a key role in regulating brain function. Excess accumulation of intracellular glutamate accelerates an increase in the concentration of reactive oxygen species (ROS) in neurons leading to glutamate neurotoxicity. In this study, we focused on the effects of glutamate on SH-SY5Y neuroblastoma cells to identify the possible alterations in oxidative stress, inflammation, and iron metabolism that affect the neurological function itself and in the presence of antioxidant lutein. First, ROS measurements were performed, and then catalase (CAT) and Superoxide Dismutase (SOD) enzyme activity were determined by enzyme activity assay kits. The ELISA technique was used to detect proinflammatory TNFα, IL-6, and IL-8 cytokine secretions. Alterations in iron uptake, storage, and release were followed by gene expression measurements and Western blotting. Total iron level detections were performed by a ferrozine-based iron detection method, and a heme assay kit was used for heme measurements. The gene expression toward lipid-peroxidation was determined by RT-PCR. Our results show glutamate changes ROS, inflammation, and antioxidant enzyme activity, modulate iron accumulation, and may initiate lipid peroxidation in SH-SY5Y cells. Meanwhile, lutein attenuates the glutamate-induced effects on ROS, inflammation, iron metabolism, and lipid peroxidation. According to our findings, lutein could be a beneficial, supportive treatment in neurodegenerative disorders.
On the Potential Role of the Antioxidant Couple Vitamin E/Selenium Taken by the Oral Route in Skin and Hair Health
Authors: Pincemail Joël , Meziane Smail
Published: November 2022

The relationship between oxidative stress and skin aging/disorders is well established. Many topical and oral antioxidants (vitamins C and E, carotenoids, polyphenols) have been proposed to protect the skin against the deleterious effect induced by increased reactive oxygen species production, particularly in the context of sun exposure. In this review, we focused on the combination of vitamin E and selenium taken in supplements since both molecules act in synergy either by non-enzymatic and enzymatic pathways to eliminate skin lipids peroxides, which are strongly implicated in skin and hair disorders.
Limited-Angle Computer Tomography with Truncated Projection Artifacts Removal
Authors: Shen Enxiang , Wang Yuxin , Yuan Jie , Carson Paul L.
Published: November 2022

Breast cancer is the most common cancer in women and the second most common cancer in the world. Digital breast tomosynthesis (DBT) is an effective medical imaging method. It can reduce the overlap of breast tissue in reconstructed images, which is beneficial to the early detection of breast cancer. DBT uses projection data from a limited range of angles and the simultaneous algebraic reconstruction technique (SART) based reconstruction method. Since the detector’s field of view (FOV) is limited, the updates of the large projection angles in SART cannot include all the voxels of the imaging target, which causes truncated projection artifacts (TPA) at the edges of the image. In this work, we use the images reconstructed by SART to perform re-projection on the virtually expanded detector panel and use a gradient calculation method to compensate for missing projection data to ensure that each update can include all the voxels. Experiments on simulation and human breast demonstrated that TPA can be effectively reduced while retaining the detailed tissue structure, thus improving the image quality at the border and recovering the obscured structural information. It might provide a better imaging result for the consequential clinical diagnosis.
Calibration and Testing of Discrete Element Modeling Parameters for Fresh Goji Berries
Authors: Yu Yang , Ren Simin , Li Jie , Chang Jiaqian , Yu Song , Sun Chao , Chen Tingmin
Published: November 2022

This paper aims at the standard grading of fresh goji berries and develops a variable gap-type fresh goji berry grading machine. To establish a complete simulation model, the discrete element parameters of the model were calibrated by a combination of physical experiments and simulation experiments. The outline of the goji berry was extracted by the SFM-CMVS technique, and a goji berry model was obtained using the multi-spherical particle model filling method in the EDEM software. By designing the free-fall, suspension collision, slope slip, and slope rolling experiments, we obtained the discrete element simulation parameters: the inter-particle collision restitution coefficient was 0.158, the collision restitution coefficient of fresh goji berry–silicone rubber material was 0.195, the static friction coefficient of fresh goji berry–silicone rubber material was 0.377, and the rolling friction coefficient of fresh goji berry–silicone rubber material was 0.063. By designing the steepest ascent search and central composite design experiments with the angle of repose (AoR) value obtained from the physical experiment as the target value (31.27°), we determined the inter-particle static friction coefficient to be 0.454 and the inter-particle rolling friction coefficient to be 0.037. Validation tests were conducted on the calibrated discrete element modeling parameters, and the results showed that the established fresh goji berry model and the optimally calibrated parameter combination are effective for discrete element studies on fresh goji berries.
Assessment of the Suitability of Non-Air-Conditioned Historical Buildings for Artwork Conservation: Comparing the Microclimate Monitoring in Vasari Corridor and La Specola Museum in Florence
Authors: Sciurpi Fabio , Carletti Cristina , Cellai Gianfranco , Piselli Cristina
Published: November 2022

The current energy crisis and the necessity to minimize energy waste suggest the need to assess non-air-conditioned buildings in terms of the need to install an air-conditioning system and to size and control it efficiently. This applies to historical museum buildings hosting artworks that require specific microclimate conditions for their preservation. With this view, this work analyzes the suitability of non-air-conditioned historical museum buildings to properly preserve exhibits. Therefore, two non-air-conditioned museums located in the historical city center of Florence, Italy, are considered as case studies, i.e., Vasari Corridor and La Specola. One year of indoor microclimate data monitored in representative rooms of the museums are analyzed according to the standard for artworks preservation and in terms of historical climate. Results of monitored indoor air temperature and relative humidity show that all monitored rooms are not suitable for the preservation of the exhibits without the installation of an air-conditioning system. However, to minimize the energy consumption, the hygrothermal control can be based on the observed historical climate that characterizes the environments, which presents acceptable preservation ranges much wider that the reference technical standard. In this way, the energy needs for the environmental control necessary to ensure the good conservation of the artworks can be significantly reduced.
Research of Bioactive Peptides in Foods
Authors: Minkiewicz Piotr
Published: November 2022

In recent years, peptides have spurred increased interest as one of the most important classes of bioactive food components [...]
Selection of Kinematic and Temporal Input Parameters to Define a Novel Upper Body Index Indicator for the Evaluation of Upper Limb Pathology
Authors: Guzik-Kopyto Agata , Nowakowska-Lipiec Katarzyna , Krysiak Mikołaj , Jochymczyk-Woźniak Katarzyna , Jurkojć Jacek , Wodarski Piotr , Gzik Marek , Michnik Robert
Published: November 2022

Purpose: This work aimed to develop a novel indicator of upper limb manipulative movements. A principal component analysis (PCA) algorithm was applied to kinematic measurements of movements of the upper limbs performed during an everyday activity. Methods: Kinematics of the upper limb while drinking from a mug were investigated using the commercially available Xsens MVN BIOMECH inertial sensor-based motion capture system. The study group consisted of 20 male patients who had previously suffered an ischaemic stroke, whilst the reference group consisted of 16 males with no disorders of their motor organs. Based on kinematic data obtained, a set of 30 temporal and kinematic parameters were defined. From this, 16 parameters were selected for the determination of a novel indicator, the Upper Body Index (UBI), which served the purpose of assessing manipulative movements of upper limbs. Selection of the 16 parameters considered the percentage distribution of the parameters beyond the standard, the differences in mean values between the reference group and the study group, and parameter variability. Results: Analysis of kinematics allowed for the identification and selection of the parameters used in the development of the new index. This included 2 temporal parameters and 14 kinematic parameters, with the minimum and maximum angles of the upper limb joints, motion ranges in the joints, and parameters connected with movement of the spine recorded. These parameters were used to assess motion in the shoulder and elbow joints, in all possible planes, as well as spine movement. The values of the UBI indicator were as follows: in the case of the reference group: 13.67 ± 2.40 for the dominant limb, 13.71 ± 3.36 for the non-dominant limb; in the case of the stroke patient group: 130.86 ± 75.07 for the dominant limb, 155.58 ± 170.76 for the non-dominant limb. Conclusions: The developed UBI made it possible to discover deviations from the standard performance of upper limb movements. Therefore, the index may be applicable to the analysis of any sequence of movements carried out by the upper limb.
A Modeling Approach for Measuring the Performance of a Human-AI Collaborative Process
Authors: Sankaran Ganesh , Palomino Marco , Knahl Martin , Siestrup Guido
Published: November 2022

Despite the unabated growth of algorithmic decision-making in organizations, there is a growing consensus that numerous situations will continue to require humans in the loop. However, the blending of a formal machine and bounded human rationality also amplifies the risk of what is known as local rationality. Therefore, it is crucial, especially in a data-abundant environment that characterizes algorithmic decision-making, to devise means to assess performance holistically. In this paper, we propose a simulation-based model to address the current lack of research on quantifying algorithmic interventions in a broader organizational context. Our approach allows the combining of causal modeling and data science algorithms to represent decision settings involving a mix of machine and human rationality to measure performance. As a testbed, we consider the case of a fictitious company trying to improve its forecasting process with the help of a machine learning approach. The example demonstrates that a myopic assessment obscures problems that only a broader framing reveals. It highlights the value of a systems view since the effects of the interplay between human and algorithmic decisions can be largely unintuitive. Such a simulation-based approach can be an effective tool in efforts to delineate roles for humans and algorithms in hybrid contexts.
Performance Evaluation of a Multidomain IMS/NGN Network Including Service and Transport Stratum
Authors: Kaczmarek Sylwester , Sac Maciej
Published: November 2022

The Next Generation Network (NGN) architecture was proposed for delivering various multimedia services with guaranteed quality. For this reason, the elements of the IP Multimedia Subsystem (IMS) concept (an important part of 4G/5G/6G mobile networks) are used in its service stratum. This paper presents comprehensive research on how the parameters of an IMS/NGN network and traffic sources influence mean Call Set-up Delay (E(CSD)) and mean Call Disengagement Delay (E(CDD)), a subset of standardized call processing performance (CPP) parameters, which are significant for both network users and operators. The investigations were performed using our analytical traffic model of a multidomain IMS/NGN network with Multiprotocol Label Switching (MPLS) technology applied in its transport stratum, which provides transport resources for the services requested by users. The performed experiments allow grouping network and traffic source parameters into three categories based on the strength of their effect on E(CSD) and E(CDD). These categories reflect the significance of particular parameters for the network operator and designer (most important, less important and insignificant).
Assessment of the Applicability of a Thawing-Guided Drainage System for Old Railway Tunnel Icicle Prevention in Cold Weather
Authors: Kang Sangjoon , Koh Taehoon , Yoo Hanju
Published: November 2022

As train failures from icicles annually recur in old railway tunnels in cold weather, fundamental measures to prevent the formation of icicles within the tunnels are necessary for the safe operation of trains and efficient maintenance of old tunnels. This study develops and tests a thawing-guided drainage system for old railway tunnels. The proposed system consists of heating panels, supporting stands, and the drainageways for discharging leaked water. A heating panel is composed of a film heater, thermal insulator, and a steel plate. The heater installed on a steel plate supplies the heat source to prevent icicle formation. This system uses a low-power module lining structure, which fundamentally prevents the formation of icicles from cracks in old railway tunnels and induces the drainage of thermally melted icicles to the outside of the track. A prototype system was installed in the Seungbu tunnel (a railway tunnel) and monitored over 3 weeks in winter from 22 December 2021 to 12 January 2022. The icicle prevention and guided drainage performances were verified at an ambient temperature of about −10 °C, with an internal temperature for maintaining the system above 0 °C over the whole test period. The proposed system is judged by the fact that the applicability of the developed system has been verified in consideration of the following three aspects: usability in the operational railway line, effectiveness through icicle prevention performance, and operational safety which does not interfere with train operations.
Natural Element Static and Free Vibration Analysis of Functionally Graded Porous Composite Plates
Authors: Cho Jin-Rae
Published: November 2022

The static bending and free vibration of functionally graded (FG) porous plates were analyzed by a 2D natural element method (NEM). Recent studies on FG materials considered the porosity because micropores and porosity were observed during the fabrication of FG materials owing to the difference in solidification temperatures. However, the mechanical responses of FG porous plates were not sufficiently revealed, and furthermore most numerical studies relied on the finite element method. Motivated by this situation, this study intended to investigate the combined effects of material composition and porosity distributions and plate thickness on the static bending and free vibration responses of ceramic–metal FG plates using 2D NEM incorporated with the (3,3,2) hierarchical model. The proposed numerical method is verified from the comparison with the reference such that the maximum relative difference is 5.336%. Five different porosity distributions are considered and the central deflection and the fundamental frequency of ceramic–metal FG porous plates are parametrically investigated with respect to the combination of the porosity parameter, the ceramic volume fraction index, and the width–thickness (w/t) ratio and to the boundary condition. The ranges of three parameters were set to 0–0.5 for the porosity, 0–0.6 for the ceramic volume fraction, and 3–20 for the width–thickness ratio. It was found from the numerical experiments that the static and free vibration responses of ceramic–metal FG porous plates are significantly affected by these parameters.