bluuebunny/crossref_metadata_2025 huggingface dataset:
Distraction force promotes the osteogenic differentiation of Gli1+ cells in facial sutures via primary cilia-mediated Hedgehog signaling pathway
Authors: Jin Mengying , An Yang , Wang Zheng , Wang Guanhuier , Lin Zhiyu , Ding Pengbing , Lu Enhang , Zhao Zhenmin , Bi Hongsen
Published: July 2024

Background

Trans-sutural distraction osteogenesis (TSDO) involves the application of distraction force to facial sutures to stimulate osteogenesis. Gli1+ cells in the cranial sutures play an important role in bone growth. However, whether Gli1+ cells in facial sutures differentiate into bone under distraction force is unknown.

Methods

4-week-old Gli1ER/Td and C57BL/6 mice were used to establish a TSDO model to explore osteogenesis of zygomaticomaxillary sutures. A Gli1+ cell lineage tracing model was used to observe the distribution of Gli1+ cells and explore the role of Gli1+ cells in facial bone remodeling.

Results

Distraction force promoted bone remodeling during TSDO. Fluorescence and two-photon scanning images revealed the distribution of Gli1+ cells. Under distraction force, Gli1-lineage cells proliferated significantly and co-localized with Runx2+ cells. Hedgehog signaling was upregulated in Gli1+ cells. Inhibition of Hedgehog signaling suppresses the proliferation and osteogenesis of Gli1+ cells induced by distraction force. Subsequently, the stem cell characteristics of Gli1+ cells were identified. Cell-stretching experiments verified that mechanical force promoted the osteogenic differentiation of Gli1+ cells through Hh signaling. Furthermore, immunofluorescence staining and RT-qPCR experiments demonstrated that the primary cilia in Gli1+ cells exhibit Hedgehog-independent mechanosensitivity, which was required for the osteogenic differentiation induced by mechanical force.

Conclusions

Our study indicates that the primary cilia of Gli1+ cells sense mechanical stimuli, mediate Hedgehog signaling activation, and promote the osteogenic differentiation of Gli1+ cells in zygomaticomaxillary sutures.
New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis
Authors: Hu Deyu , Lai Jiejuan , Chen Quanyu , Bai Lianhua
Published: July 2024

Background

Bone marrow-derived mesenchymal stem cell (BMMSC)-based therapy has become a major focus for treating liver fibrosis/cirrhosis. However, although these cell therapies promote the treatment of this disease, the heterogeneity of BMMSCs, which causes insufficient efficacy during clinical trials, has not been addressed. In this study, we describe a novel Percoll–Plate–Wait procedure (PPWP) for the isolation of an active cell subset from BMMSC cultures that was characterized by the expression of neuroglial antigen 2 (NG2/BMMSCs).

Methods

By using the key method of PPWP and other classical biological techniques we compared NG2/BMMSCs with parental BMMSCs in biological and functional characteristics within a well-defined diethylnitrosamine (DEN)-induced liver fibrosis/cirrhosis injury male C57BL/6 mouse model also in a culture system. Of note, the pathological alterations in the model is quite similar to humans’.

Results

The NG2/BMMSCs revealed more advantages compared to parentalBMMSCs. They exhibited greater proliferation potential than parental BMMSCs, as indicated by Ki-67 immunofluorescence (IF) staining. Moreover, higher expression of SSEA-3 (a marker specific for embryonic stem cells) was detected in NG2/BMMSCs than in parental BMMSCs, which suggested that the “stemness” of NG2/BMMSCs was greater than that of parental BMMSCs. In vivo studies revealed that an injection of NG2/BMMSCs into mice with ongoing DEN-induced liver fibrotic/cirrhotic injury enhanced repair and functional recovery to a greater extent than in mice treated with parental BMMSCs. These effects were associated with the ability of NG2/BMMSCs to differentiate into bile duct cells (BDCs). In particular, we discovered for the first time that NG2/BMMSCs exhibit unique characteristics that differ from those of parental BMMSCs in terms of producing liver sinusoidal endothelial cells (LSECs) to reconstruct injured blood vessels and sinusoidal structures in the diseased livers, which are important for initiating hepatocyte regeneration. This unique potential may also suggest that NG2/BMMSCs could be an novel off-liver progenitor of LSECs. Ex vivo studies revealed that the NG2/BMMSCs exhibited a similar trend to that of their in vivo in terms of functional differentiation responding to the DEN-diseased injured liver cues. Additionally, the obvious core role of NG2/BMMSCs in supporting the functions of BMMSCs in bile duct repair and BDC-mediated hepatocyte regeneration might also be a novel finding.

Conclusions

Overall, the PPWP-isolated NG2/BMMSCs could be a novel effective cell subset with increased purity to serve as a new therapeutic tool for enhancing treatment efficacy of BMMSCs and special seed cell source (BDCs, LSECs) also for bioliver engineering.
Comparative effectiveness of mesenchymal stem cell versus bone-marrow mononuclear cell transplantation in heart failure: a meta-analysis of randomized controlled trials
Authors: Hosseinpour Alireza , Kamalpour Jahangir , Dehdari Ebrahimi Niloofar , Mirhosseini Seyed Alireza , Sadeghi Alireza , Kavousi Shahin , Attar Armin
Published: July 2024

Background

There is no clear evidence on the comparative effectiveness of bone-marrow mononuclear cell (BMMNC) vs. mesenchymal stromal cell (MSC) stem cell therapy in patients with chronic heart failure (HF).

Methods

Using a systematic approach, eligible randomized controlled trials (RCTs) of stem cell therapy (BMMNCs or MSCs) in patients with HF were retrieved to perform a meta-analysis on clinical outcomes (major adverse cardiovascular events (MACE), hospitalization for HF, and mortality) and echocardiographic indices (including left ventricular ejection fraction (LVEF)) were performed using the random-effects model. A risk ratio (RR) or mean difference (MD) with corresponding 95% confidence interval (CI) were pooled based on the type of the outcome and subgroup analysis was performed to evaluate the potential differences between the types of cells.

Results

The analysis included a total of 36 RCTs (1549 HF patients receiving stem cells and 1252 patients in the control group). Transplantation of both types of cells in patients with HF resulted in a significant improvement in LVEF (BMMNCs: MD (95% CI) = 3.05 (1.11; 4.99) and MSCs: MD (95% CI) = 2.82 (1.19; 4.45), between-subgroup p = 0.86). Stem cell therapy did not lead to a significant change in the risk of MACE (MD (95% CI) = 0.83 (0.67; 1.06), BMMNCs: RR (95% CI) = 0.59 (0.31; 1.13) and MSCs: RR (95% CI) = 0.91 (0.70; 1.19), between-subgroup p = 0.12). There was a marginally decreased risk of all-cause death (MD (95% CI) = 0.82 (0.68; 0.99)) and rehospitalization (MD (95% CI) = 0.77 (0.61; 0.98)) with no difference among the cell types (p > 0.05).

Conclusion

Both types of stem cells are effective in improving LVEF in patients with heart failure without any noticeable difference between the cells. Transplantation of the stem cells could not decrease the risk of major adverse cardiovascular events compared with controls. Future trials should primarily focus on the impact of stem cell transplantation on clinical outcomes of HF patients to verify or refute the findings of this study.
New advances in drug development for metabolic dysfunction-associated diseases and alcohol-associated liver disease
Authors: Zhang Jinming , Li Yixin , Yang Liu , Ma Ningning , Qian Shengying , Chen Yingfen , Duan Yajun , Xiang Xiaogang , He Yong
Published: July 2024

Metabolic disorders are currently threatening public health worldwide. Discovering new targets and developing promising drugs will reduce the global metabolic-related disease burden. Metabolic disorders primarily consist of lipid and glucose metabolic disorders. Specifically, metabolic dysfunction-associated steatosis liver disease (MASLD) and alcohol-associated liver disease (ALD) are two representative lipid metabolism disorders, while diabetes mellitus is a typical glucose metabolism disorder. In this review, we aimed to summarize the new drug candidates with promising efficacy identified in clinical trials for these diseases. These drug candidates may provide alternatives for patients with metabolic disorders and advance the progress of drug discovery for the large disease burden.
The effectiveness and safety of acupoint herbal patching for allergic rhinitis: protocol for a systematic review and meta-analysis
Authors: Xu Ningcong , Zhang Claire Shuiqing , Tan Xi , Lai Yunjie , Cen Yanfang , Zhou Shiqing , Xia Jiyan , Li Yunying , Luo Qiulan
Published: July 2024

Background

Allergic rhinitis (AR) is a common inflammatory disease of the nasal mucosa that is characterized by symptoms such as sneezing, nasal congestion, nasal itching, and rhinorrhoea. In recent years, acupoint herbal patching (AHP) therapy has gained a growing interest as a potential management option for AR. This systematic review and meta-analysis will evaluate the clinical research evidence on the effectiveness and safety of AHP as a treatment option for AR outside of the Sanfu or Sanjiu days (summer or winter solstice). The results of this review will provide up-to-date evidence-based guidance for healthcare providers and individuals seeking alternative treatments for AR.

Methods

A comprehensive search of electronic databases (PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), EMBASE, China National Knowledge Infrastructure (CNKI), CQVIP, Sino-Med, and Wanfang Databases) will be conducted from their inception to June 2023. The inclusion criteria will be limited to randomized controlled trials that evaluate the effectiveness or efficacy of non-Sanfu or non-Sanjiu AHP for AR. The primary outcome measure will be the total nasal symptom score. The methodological quality of included studies will be assessed using the Revised Cochrane risk-of-bias tool for randomized trials (RoB 2), and meta-analyses will be performed using RevMan (V.5.3) statistical software. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach will be used to determine the certainty of evidence.

Discussion

This systematic review and meta-analysis will provide valuable insights into the effectiveness and safety of non-Sanfu or non-Sanjiu AHP as a treatment option for AR. The study aims to produce a high-quality review by adhering to PRISMA-P guidelines and using clinical guideline recommended outcome measures. The results of this review may offer additional treatment options for AR patients who seek complementary and alternative therapies, and hold significant implications for future research in this field. Overall, this study has the potential to inform clinical practice and improve patient outcomes.

Systematic review registration

PROSPERO CRD42022181322.
Fecal microbiota transplantation alters gut phage communities in a clinical trial for obesity
Authors: Zuppi Michele , Vatanen Tommi , Wilson Brooke C. , Golovina Evgeniia , Portlock Theo , Cutfield Wayne S. , Vickers Mark H. , O’Sullivan Justin M.
Published: July 2024

Background

Fecal microbiota transplantation (FMT) is a therapeutic intervention used to treat diseases associated with the gut microbiome. In the human gut microbiome, phages have been implicated in influencing human health, with successful engraftment of donor phages correlated with FMT treatment efficacy. The impact that gastrointestinal phages exert on human health has primarily been connected to their ability to modulate the bacterial communities in the gut. Nonetheless, how FMT affects recipients’ phage populations, and in turn, how this influences the gut environment, is not yet fully understood. In this study, we investigated the effects of FMT on the phageome composition of participants within the Gut Bugs Trial (GBT), a double-blind, randomized, placebo-controlled trial that investigated the efficacy of FMT in treating obesity and comorbidities in adolescents. Stool samples collected from donors at the time of treatment and recipients at four time points (i.e., baseline and 6 weeks, 12 weeks, and 26 weeks post-intervention), underwent shotgun metagenomic sequencing. Phage sequences were identified and characterized in silico to examine evidence of phage engraftment and to assess the extent of FMT-induced alterations in the recipients’ phageome composition.

Results

Donor phages engrafted stably in recipients following FMT, composing a significant proportion of their phageome for the entire course of the study (33.8 ± 1.2% in females and 33.9 ± 3.7% in males). Phage engraftment varied between donors and donor engraftment efficacy was positively correlated with their phageome alpha diversity. FMT caused a shift in recipients’ phageome toward the donors’ composition and increased phageome alpha diversity and variability over time.

Conclusions

FMT significantly altered recipients' phage and, overall, microbial populations. The increase in microbial diversity and variability is consistent with a shift in microbial population dynamics. This proposes that phages play a critical role in modulating the gut environment and suggests novel approaches to understanding the efficacy of FMT in altering the recipient’s microbiome.

Trial registration

The Gut Bugs Trial was registered with the Australian New Zealand Clinical Trials Registry (ACTR N12615001351505). Trial protocol: the trial protocol is available at https://bmjopen.bmj.com/content/9/4/e026174.
Cultivation of novel Atribacterota from oil well provides new insight into their diversity, ecology, and evolution in anoxic, carbon-rich environments
Authors: Jiao Jian-Yu , Ma Shi-Chun , Salam Nimaichand , Zhou Zhuo , Lian Zheng-Han , Fu Li , Chen Ying , Peng Cheng-Hui , OuYang Yu-Ting , Fan Hui , Li Ling , Yi Yue , Zhang Jing-Yi , Wang Jing-Yuan , Liu Lan , Gao Lei , Oren Aharon , Woyke Tanja , Dodsworth Jeremy A. , Hedlund Brian P. , Li Wen-Jun , Cheng Lei
Published: July 2024

Background

The Atribacterota are widely distributed in the subsurface biosphere. Recently, the first Atribacterota isolate was described and the number of Atribacterota genome sequences retrieved from environmental samples has increased significantly; however, their diversity, physiology, ecology, and evolution remain poorly understood.

Results

We report the isolation of the second member of Atribacterota, Thermatribacter velox gen. nov., sp. nov., within a new family Thermatribacteraceae fam. nov., and the short-term laboratory cultivation of a member of the JS1 lineage, Phoenicimicrobium oleiphilum HX-OS.bin.34TS, both from a terrestrial oil reservoir. Physiological and metatranscriptomics analyses showed that Thermatribacter velox B11T and Phoenicimicrobium oleiphilum HX-OS.bin.34TS ferment sugars and n-alkanes, respectively, producing H2, CO2, and acetate as common products. Comparative genomics showed that all members of the Atribacterota lack a complete Wood-Ljungdahl Pathway (WLP), but that the Reductive Glycine Pathway (RGP) is widespread, indicating that the RGP, rather than WLP, is a central hub in Atribacterota metabolism. Ancestral character state reconstructions and phylogenetic analyses showed that key genes encoding the RGP (fdhA, fhs, folD, glyA, gcvT, gcvPAB, pdhD) and other central functions were gained independently in the two classes, Atribacteria (OP9) and Phoenicimicrobiia (JS1), after which they were inherited vertically; these genes included fumarate-adding enzymes (faeA; Phoenicimicrobiia only), the CODH/ACS complex (acsABCDE), and diverse hydrogenases (NiFe group 3b, 4b and FeFe group A3, C). Finally, we present genome-resolved community metabolic models showing the central roles of Atribacteria (OP9) and Phoenicimicrobiia (JS1) in acetate- and hydrocarbon-rich environments.

Conclusion

Our findings expand the knowledge of the diversity, physiology, ecology, and evolution of the phylum Atribacterota. This study is a starting point for promoting more incisive studies of their syntrophic biology and may guide the rational design of strategies to cultivate them in the laboratory.
Diagnostic efficiency of intravoxel incoherent motion-based virtual magnetic resonance elastography in pulmonary neoplasms
Authors: Zhang Shuo , Du Yonghao , Liang Ting , Zhang Xuyin , Guo Yinxia , Yang Jian , Li Xianjun , Niu Gang
Published: July 2024

Background

The aim of the study were as below. (1) To investigate the feasibility of intravoxel incoherent motion (IVIM)-based virtual magnetic resonance elastography (vMRE) to provide quantitative estimates of tissue stiffness in pulmonary neoplasms. (2) To verify the diagnostic performance of shifted apparent diffusion coefficient (sADC) and reconstructed virtual stiffness values in distinguishing neoplasm nature.

Methods

This study enrolled 59 patients (37 males, 22 females) with one pulmonary neoplasm who underwent computed tomography-guided percutaneous transthoracic needle biopsy (PTNB) with pathological diagnosis (26 adenocarcinoma, 10 squamous cell carcinoma, 3 small cell carcinoma, 4 tuberculosis and 16 non-specific benign; mean age, 60.81 ± 9.80 years). IVIM was performed on a 3 T magnetic resonance imaging scanner before biopsy. sADC and virtual shear stiffness maps reflecting lesion stiffness were reconstructed. sADC and virtual stiffness values of neoplasm were extracted, and the diagnostic performance of vMRE in distinguishing benign and malignant and detailed pathological type were explored.

Results

Compared to benign neoplasms, malignant ones had a significantly lower sADC and a higher virtual stiffness value (P < 0.001). Subsequent subtype analyses showed that the sADC values of adenocarcinoma and squamous cell carcinoma groups were significantly lower than non-specific benign group (P = 0.013 and 0.001, respectively). Additionally, virtual stiffness values of the adenocarcinoma and squamous cell carcinoma subtypes were significantly higher than non-specific benign group (P = 0.008 and 0.001, respectively). However, no significant correlation was found among other subtype groups.

Conclusions

Non-invasive vMRE demonstrated diagnostic efficiency in differentiating the nature of pulmonary neoplasm. vMRE is promising as a new method for clinical diagnosis.
Effect of altrenogest treatment before weaning on reproductive performance and production efficiency in primiparous and multiparous sows
Authors: Liu Kexiong , Xu Xiaoling , Song Yuqing , Xiao Linli , Wen Junhui , Ding Haiquan , Zhao Shuxin , Qiao Dongliang , Zhang Bo , Niu Aihua , Bai Jiahua , Liu Yan
Published: July 2024

Background

Most sows will experience negative energy balance during lactation resulting in impaired follicular development. This study aimed to treat 28-day lactating sows with altrenogest (ALT) to suppress follicle enlargement during lactation, and to assess the estrus and reproductive performance post-weaning.

Methods

In this study, we conducted two trials. In trial 1, we monitored the follicular development of lactating sows including 10 primiparous sows and 10 multiparous sows during the whole lactation to confirm the ALT administration time. In trial 2, a total of 42 primiparous and 111 multiparous sows were allocated to three treatments: Ctrl (control group,n = 51): no treatment; TAI (timed artificial insemination group,n = 51): sows were injected with equine chorionic gonadotropin (eCG) after weaning 24 h and gonadotropin-releasing hormone (GnRH) when they expressed estrus; and AT-TAI (ALT treatment-timed artificial insemination group,n = 51): base on the process of TAI group, the sows were fed with 20 mg ALT per day before weaning 10 days. All sows were artificially inseminated twice at 12 h and 36 h after estrus. The follicle size changes and serum hormone levels were explored in this process.

Results

Although the follicle size of multiparous sows was larger than primiparous sows during the whole lactation (P < 0.05), similar change trends of follicle size were observed in primiparous and multiparous sows. Meanwhile, the FSH, LH and E2levels of multiparous sows were higher than primiparous sows. The ALT treatment significantly inhibits the increase in follicle size (P < 0.05) and reduces the serum levels of FSH, LH and E2(P > 0.05). Additionally, ALT treatment increases estrus concentration and the preovulatory follicle size (P < 0.05), meanwhile, it delays the weaning-to-estrus interval (WEI,P < 0.001). However, the estrus rate, pregnancy rate, total pigs born and born alive did not differ between treatments (P > 0.05).

Conclusions

There were significant differences in the size of follicles in the lactation between primiparous and multiparous sows. ALT treatment during the last ten days of lactation concentrated estrus expression leading to higher work efficiency of breeder in batch production, however, with no improvement in reproductive performance.
Identifying transitions in corporate sustainability reporting: a content analysis of JSE/FTSE multinational sustainability reports from 2016 to 2021
Authors: Kassier Liesel
Published: July 2024

The dominant practice governing sustainability reporting in the private sector is that of Corporate Social Responsibility (CSR) or Environmental Social Governance (ESG) reporting. CSR has its roots in philanthropy and charitable initiatives, while ESG aims to integrate environmental, social, and governance factors into business practices and decision-making. This paper analyses the transition in sustainability worldviews revealed in corporate sustainability reporting from 2016 to 2021. It uses a longitudinal content analysis methodology applied to a sample of ten multinational companies listed on the South African JSE/FTSE top 40 index. The period for the longitudinal study is framed from when the companies started reporting on ESG. The JSE/FTSE was chosen as the companies listed in the top 40 represent 80% of the value on the JSE (JSE 2020). The qualitative content analysis makes use of the five stages of corporate sustainability model to position companies’ sustainability reports within these five stages (Landrum & Ohsowski, 2018a). The key finding of this paper is that multinational companies have been slow to transition their sustainability reporting practices. The current reports reflect a business-as-usual mindset that is driven by compliance with reporting regulations. There is an absence of reporting that reflects a view of embedding business operations within bounded science-based ecological and social environments.
Medical and nursing interventions influence a caregiver’s willingness to continue home hemodialysis: a multicenter cross-sectional survey
Authors: Kuroda Saori , Yabe Hiroki , Nagata Arisa , Imoto Chiaki , Oida Miwa , Kadoshima Yoko , Fudoji Miki , Miyashita Yoshiko , Nakai Shigeru , Masakane Ikuto
Published: July 2024

Background

In Japan, home hemodialysis (HHD) has several benefits, including a favorable prognosis, enhanced quality of life (QOL) of patients, and cost-effectiveness. However, the caregiver’s burden in assisting patients on HHD is significant, which influences their decision to continue HHD. This study focused on the relationship between medical and nursing interventions, caregiver resolve to continue HHD, and caregiver quality of life, aiming to create support strategies for caregivers.

Methods

A multicenter cross-sectional survey was conducted on caregivers of patients on HHD from 150 HHD facilities in Japan. The caregiver’s intent to continue HHD was assessed using a four-tier response scale that evaluated aspects such as caregiver–healthcare provider meetings, caregiver breaks, treatment discussions, difficulty in HHD initiation, and post-initiation medical support. Caregiver QOL was evaluated using the Short-form Health Survey, eight domains (SF-8). Statistical analysis involved χ-squared tests, independent t-tests, and propensity score matching.

Results

The study achieved a caregiver participation rate of 55.6%. Results indicated that caregivers who were inclined to continue HHD were significantly more likely to receive respite outpatient dialysis and had a better understanding of HHD mechanics as explained by healthcare professionals; however, propensity score matching revealed limited generalizability. There was no significant difference in the SF-8 scores before and after propensity score matching among the groups.

Conclusions

This study highlights that there are a small number of caregivers who have little motivation to continue HHD and emphasizes the crucial role of support from healthcare professionals. Comprehensive support, including respite dialysis and detailed HHD information, may affect HHD sustainability.
Which learning techniques supported by cognitive research do students use at secondary school? Prevalence and associations with students’ beliefs and achievement
Authors: Ruiz-Martín Héctor , Blanco Fernando , Ferrero Marta
Published: July 2024

Research in cognitive science has highlighted the effectiveness of several learning techniques, and a number of studies have analyzed their prevalence among university students and their relationship with academic achievement. In this study, we surveyed a large, heterogeneous sample of secondary school students to reveal how often they use research-supported techniques in comparison with other frequent techniques, and we analyzed the association between their study strategies and school achievement. We also assessed the associations between study techniques and several students’ beliefs and attitudes toward learning (self-efficacy, goal orientation, control beliefs, growth mindset, and examination anxiety). Results showed that, except for distributed practice, only those techniques that are supported by previous research yielded an association with achievement, and they exhibited higher associations with self-efficacy, growth mindset, control beliefs, and learning goal orientation than non-supported techniques.
Morphologic and phylogenetic investigations revealed size-divergent clades in chelae morphotypes of freshwater prawn Macrobrachium vollenhovenii Herklots (Decapoda: Palaemonidae) in a lake and river system of Southwest Nigeria
Authors: Oyebola Oyediran Olusegun. , Coker Oluwakayode Michael , Oche Charity , Akanmu Olumuyiwa Ayodeji , Alamu Oluwatoyin , Azuh Victor Okechukwu , Idowu Christy Funmilayo
Published: July 2024

Background

The freshwater prawn Macrobrachium vollenhovenii is one of the largest Macrobrachium species, a biological agent against human schistosomiasis, and a cheap protein source in riverine communities in West Africa. However, its aquaculture development for sustainable utilization is challenged by cryptic identity amidst the presence of morphotypes of unknown size and genetic relatedness. This study aimed to investigate the maximum sizes and evolutionary links of chelae morphotypes in M. vollenhovenii for precise identification and utilization in a 3 × 2 randomized block experimental design. Ninety biggest encountered samples of M. vollenhovenii chelae morphotypes—those possessing equal left and right side chelae, longer left chelae, and shorter left chelae—were obtained from fisherfolks’ catches at each of Asejire Lake and Ogun River during peak seasons (July–September) bimonthly field survey, representing EAAL, LLAL, SLAL—GAALs, and EAOR, LLOR, SLOR—GAORs. These were analyzed for differences (p < 0.05) in size-linked parameters—length (L (cm)), weight (W (g)), and condition factor (K). Specimens’ 16S rRNA nucleotide sequences were utilized to infer phylogenetic linkages, single-nucleotide polymorphism (SNP), and amino acid translations alongside NCBI references (NCBIrefseq).

Results

Weight (W) and condition factor (K), among GAALs, for SLAL and LLAL were similar; EAAL was significantly lowest; among GAORs, SLOR and LLOR were similar; and EAOR was significantly lowest. In GAALs, EAAL, LLAL, and SLAL had higher L, W, and K than counterpart GAORs. Sequences formed two polyphyletic groups: EAOR branch from EAAL, in which 100.0%EA rooted 75.0% NCBIrefseqs, forming a clade; and GAOR-SLOR and LLOR branch form GAOR-LLAL rooted SLAL, in which 100.0%LL and SL rooted KJ463387.1 (Badagry), forming another clade. SNP Locus 91 separated 100.0%GAOR from 100.0%GAAL and 100.0%NCBIrefseqs translating to valine; SNP Locus 171 separated 100.0%EA and its co-rooted NCBIrefseqs from 100.0%LL, SL, and their co-rooted NCBIrefseq, translating to glycine/glutamic acid change.

Conclusion

The equal left and right side chelae and the unequal left and right side chelae specimens are, respectively, small- and robust-sized, irrespective of habitat. They are divergent size-linked clades having protein translate differences, delineable at 16S rRNA SNP Locus 171; their size variant habitat strains are delineable at SNP Locus 91. These SNP markers will be useful for precision identification and selection of the size variant chelae morph strains for sustainable utilization.
Functional characteristics and storage stability of hot air assisted radio frequency treated pearl millet
Authors: Yarrakula Srinivas , Mummaleti Gopinath , Toleti Kavya Sree , Saravanan Shanmugasundaram
Published: July 2024

Nutricereals, pearl millet flour consumption is hardly limited to a few specific regions of the world owing to the development of early rancidity on storage. Hot air assisted radio frequency technology (HARF) was used to improve the storage stability of pearl millet flour. Pearl millets at different moisture levels of 10.5±0.5, 12 & 15% were subjected to HARF for 5-, 10- & 15-min exposure period under fixed electrode position. The results revealed that significant reduction in peroxide value and free fatty acid values of flours from treated pearl millet was found compared to decorticated raw flour; thereby, the storage stability enhancement of pearl millet flour up to 180 days was achieved. No significant difference was found for emulsifying, foaming and cooking properties between treated pearl millet at 15% moisture, 15 min exposure and decorticated raw one. In contrast, the bulk density of flour from treated pearl millet was found higher. Significant increase in hardness of cooked grains after the treatment was observed while the springiness, cohesiveness and gumminess values did not vary. In FTIR analysis, no difference was observed between the peaks of whole and decorticated pearl millet flours in both untreated and treated samples.

Graphical Abstract
Impact of SARS-CoV-2 on male reproductive system and fertility
Authors: Sajjad Solmaz Gul , Sajjad Aarush Mohammad , Fakih Michael , Akhtar Muhammad Ahsan , Sajjad Yasmin
Published: July 2024

Objective

Evaluate the impact of SARS-CoV-2 on male fertility.

Design

Comprehensive analysis of studies exploring virus presence, inflammation, and altered semen parameters.

Result(s)

While some studies report absence of SARS-CoV-2 in semen, others highlight testicular injury, inflammation, and potential viral orchitis. Scrotal discomfort and altered sperm parameters indicate reproductive implications.

Conclusion

SARS-CoV-2 may pose significant challenges to male fertility, necessitating further research for a comprehensive understanding of its long-term effects. Limitations include varied testing methods and sample sizes, emphasizing the need for confirmation and detailed post-COVID examinations.
From dyspnea to diagnosis, unmasking undifferentiated cardiac sarcoma: a case report
Authors: Guerra-Raygada Mauricio , Saavedra-Sanchez Alee Jonhson , Hidalgo-Avendaño Diego , Bermudez-Pelaez Milagros F. , Guevara-Lazo David , Nombera-Aznaran Natalia
Published: July 2024

Background

Sarcomas are the most common type of cardiac malignancy, but they are extremely rare. Within this group, angiosarcomas have the highest frequency, followed by undifferentiated sarcomas. This type of tumor has a poor prognosis and a high recurrence rate. Information about these tumors is limited, relying mainly on case reports and autopsy series. The purpose of this case report is to detail the multifaceted approach to diagnosing and managing an undifferentiated cardiac sarcoma and contribute to the literature.

Case presentation

A 28-year-old man presented with dyspnea and chest pain, which had developed progressively over several weeks. Physical examination revealed low blood pressure, elevated heart rate, and diminished heart sounds. Imaging, including a CT scan, identified a hypodense mass in the right ventricle. Further evaluation through echocardiograms and contrast angiotomography confirmed a mass causing right ventricular obstruction. Part of the tumor was surgically removed and diagnosed  as cardiac sarcoma.  Histopathological analysis of the mass showed an undifferentiated cardiac sarcoma.

Conclusion

This case underscores the significance of including cardiac tumors as a potential cause when diagnosing cardiac masses. It also demonstrates the poor prognosis and tendency for recurrence, while revealing the absence of established management guidelines.
Evaluating the effect of different diaphragm wall cracking scenarios on seepage and slope stability in Earth dams using experimental and numerical modeling approaches
Authors: Fawzy Momen Atef , Hassan Nagy Ali Ali , Saad Neveen Yousif , El-Molla Doaa Anas
Published: July 2024

Background

Seepage control measures are essential in earth dams to enhance their safety against seepage and slope failures. A diaphragm is a thin impervious wall that is used in the core area of the dam to prevent the seepage of water. Any crack in the diaphragm can reduce its efficiency and threaten the safety and stability of the dam. Extensive evaluation of the influence of diaphragm fracturing on dam safety is required to establish a safe design and offer the appropriate measures. This study investigates the effect of diaphragm cracking on the safety of the dam for seepage and slope stability. A permeability tank is first used to validate the numerical model; then, different scenarios for cracked diaphragm walls are studied numerically. The crack’s height, width, direction, and number are all investigated. The effect of having a downstream horizontal blanket drain with cracked internal diaphragms is also evaluated.

Results

The results showed that the internal diaphragm enhances the safety of earth dams as it reduced the seepage discharge by 98% and increased the downstream slope safety by 34%. Considering the development of a crack in the internal diaphragm, the most critical location was at the base of the dam, wider cracks were more destructive, and the most critical direction was the horizontal crack. The fracturing of the diaphragm at multiple locations proved to be detrimental to the safety of the dam. Having three cracks caused the seepage discharge to reach 74% of its value for no diaphragm, the pore water pressure to raise drastically, and the downstream critical factor of safety to be higher than the case of no diaphragm by 8% only.

Conclusions

According to the findings, it is advised to continuously monitor the components of diaphragm-type earth dams and perform the necessary maintenance works whenever any sign of damage or water seepage appears. Furthermore, using a well-graded horizontal drain of sufficient length and high permeability with the internal diaphragm is highly recommended as a mitigation measure if diaphragm fracturing occurs. This helps to quickly release the seeping water and keep the phreatic line away from the downstream slope.

Graphical abstract
The gendered nature of household decision making and expenditure choices in the context of smallholder agricultural commercialization in Malawi
Authors: Msofi Mgalamadzi Loveness , Matita Mirriam , Chimombo Masautso
Published: July 2024

Background

The push towards commercialization is driven by the modernization paradigm which argues that traditional subsistence farming is backward and primitive. Despite commercialization having the potential to enhance people’s livelihoods, it can also bring unfathomable social differentiation and widen inequalities in communities. This paper investigates the gendered decision-making and expenditure choices of smallholder households in the context of agricultural commercialization in Central Malawi.

Methods

The analysis is performed by employing sequential mixed methods of quantitative and qualitative data collection, consisting of 28 focus group discussions, 100 life histories, and 519 surveys. Qualitative data were analysed using thematic content analysis while quantitative data were analyzed using descriptive and various regression estimations.

Results

The results show that many men heads make decisions on cultivated plots and control realized income. The results further show that there are no differences between households headed by women and those headed by men in pursuit of markets. Women’s participation in markets is limited by lack of means of production. Men are getting more involved in crops that were dominated by women such as groundnuts. Women plot managers engaged more with markets, but their control of realized income was less associated with reinvesting in crop commercialization which is also reflected in their expenditure choices. When they control income, women spend relatively less than men on both productive and households assets. In households headed by men, the men dominate selling of produce, decisions on the control over resources, income, and expenditure choices, which results in deepening gender inequalities.

Conclusions

Differences exist in expenditure choices between women and men which points to differing priorities and disparities in access to productive resources. Men dominate decision-making which is preferred by some women to maintain intrahousehold harmony. There is need to create targeted programs for diverse groups of women through participatory approaches to address strategic needs. Women unable to farm due to lack of means of production need social protection programs. Women without decision-making power require empowerment initiatives and gender dialogue sessions to foster joint household activities. Commercial based interventions should go beyond mere targeting of women and men but embed equality interventions to avoid deepening social inequalities.
Solar energy EV charging system using integrated Zeta-Luo converter
Authors: Kancherla Ananda Babu , Prasad N. Bhanu , Kishore D. Ravi
Published: July 2024

This paper focuses on a grid-incorporated solar electric vehicle (EV) charging station that maximizes the acceptance of EVs in agricultural areas and reduces the over-reliance on the grid of urban cities. Since photovoltaic (PV) systems are widely available and easy to install, they are an excellent choice for EV charging applications. Hence, the aim of this work is to combine PV and EV, in order to achieve the objectives of both decarbonized energy generation and transportation. A Modified Zeta Integrated Luo Converter (MZILC), which provides a large conversion range combined with lower voltage stress, is proposed intending to increase the voltage level of the PV system. Furthermore, the converter generates a stable output devoid of fluctuations with the usage of the Grey Wolf–Grasshopper Optimized Proportional Integral (GW-GOPI) controller. Electricity for BLDC motors is generated by PV power during the day and supplied by a single-phase grid at night. The efficiency of the suggested grid-connected PV-based EV charging station is examined in MATLAB environment.
Hybrid air conditioning and seawater desalination system assisted by solar energy: thermoeconomic investigation and optimization
Authors: Yosry Nashwa , Elgendy Essam , Mostafa Ahmed , Fatouh Mohamed
Published: July 2024

The demand for freshwater has become progressively critical due to population growth and water scarcity. The current work investigates the energetic performance of a hybrid absorption refrigeration and desalination system. A parametric study for the system operating conditions has been performed to get the optimal conditions. Also, economic analysis and a multi-objective optimization method are used with the aim of maximizing the energy utilization factor of the system and minimizing total costs. The specific cost of desalinated water from the multi-objective optimization is calculated as (1.728 Cent/L) with the greatest energy utilization factor of (2.214). It turns out that, the system performs optimum at condensation and generation temperatures of 30 and 65 °C respectively, with resultant COP and GOR of 0.84 and 1.37. Using the optimum conditions, TRNSYS has been used to investigate the transient performance of the system for Marsa–Matrouh City in Egypt. The cooling and freshwater demands are 10 kW and 24 L/h. It was concluded that the input energy required to run the hybrid system decreased by about 65% less than that required for separate systems. With the assistance of solar energy, it became clear that the energy required was 60% less than the hybrid system as well.
Perfusion deficits may underlie lung and kidney injury in severe COVID-19 disease: insights from a multicenter international cohort study
Authors: Nova Alice , McNicholas Bairbre , Magliocca Aurora , Laffey Matthew , Zambelli Vanessa , Mariani Ilaria , Atif Minahel , Giacomini Matteo , Vitale Giovanni , Rona Roberto , Foti Giuseppe , Laffey John , Rezoagli Emanuele , O’Connor Aine , Giani Marco , Pozzi Matteo , Coppadoro Andrea , Sordi Silvia , Crippa Ilaria Alice ,   The LUNG-KIDNEY Interaction Multicenter Study Group
Published: July 2024

Background

Lung perfusion defects, mainly due to endothelial and coagulation activation, are a key contributor to COVID-19 respiratory failure. COVID-19 patients may also develop acute kidney injury (AKI) because of renal perfusion deficit. We aimed to explore AKI-associated factors and the independent prediction of standardized minute ventilation (MV)—a proxy of alveolar dead space—on AKI onset and persistence in COVID-19 mechanically ventilated patients.

Methods

This is a multicenter observational cohort study. We enrolled 157 COVID-19 patients requiring mechanical ventilation and intensive care unit (ICU) admission. We collected clinical information, ventilation, and laboratory data. AKI was defined by the 2012 KDIGO guidelines and classified as transient or persistent according to serum creatinine criteria persistence within 48 h. Ordered univariate and multivariate logistic regression analyses were employed to identify variables associated with AKI onset and persistence.

Results

Among 157 COVID-19 patients on mechanical ventilation, 47% developed AKI: 10% had transient AKI, and 37% had persistent AKI. The degree of hypoxia was not associated with differences in AKI severity. Across increasing severity of AKI groups, despite similar levels of paCO2, we observed an increased MV and standardized MV, a robust proxy of alveolar dead space. After adjusting for other clinical and laboratory covariates, standardized MV remained an independent predictor of AKI development and persistence. d-dimer levels were higher in patients with persistent AKI.

Conclusions

In critically ill COVID-19 patients with respiratory failure, increased wasted ventilation is independently associated with a greater risk of persistent AKI. These hypothesis-generating findings may suggest that perfusion derangements may link the pathophysiology of both wasted ventilation and acute kidney injury in our population.
Targeting the Manifestations of Subclinical and Overt Hypothyroidism Within the Hippocampus
Authors: Rovet Joanne F
Published: July 2024

Background

The past decade has witnessed a surge of articles describing the neurocognitive sequelae and associated structural and functional brain abnormalities of patients with overt hypothyroidism (OH) and subclinical hypothyroidism (SCH). Findings show effects primarily within the frontal lobes with usually worse outcomes for OH than SCH. Several recent studies have also indicated hypothyroid patients may have smaller hippocampi, a key structure for memory.

Context

The JCEM paper by Zhang and colleagues applies 2 novel approaches for analyzing hippocampal structure and function. One uses an automated processing tool that segments the hippocampus into distinct subregions, and the other performs connectivity analysis to assess the relationships between specific hippocampal subregions and cortical areas. Relatively large samples of OH and SCH patients and healthy controls received a test of global cognitive functioning and underwent structural and functional magnetic resonance imaging. Results showed hypothyroid groups scored significantly below controls on the memory scale and also had smaller hippocampal volumes in selective subregions. Effects were stronger for SCH than OH groups, who also showed different patterns of interconnectivity between hippocampal subregions and specific frontal lobe areas.

Interpretation

To make sense of these findings, I explored the rodent and human literatures on thyroid hormone's role in hippocampal functioning and on hippocampal subfields and their purported functions and interconnections. Because current results suggest SCH may represent a distinct clinical entity with unique brain manifestations, I hypothesized 2 explanations for these findings, one involving transporter defects in the brain barriers and the other, differential neurodegeneration of the blood-brain barrier vascular unit.
Beta-Hydroxybutyrate Promotes Basal Insulin Secretion While Decreasing Glucagon Secretion in Mouse and Human Islets
Authors: Banerjee Risha , Zhu Ying , Brownrigg George P , Moravcova Renata , Rogalski Jason C , Foster Leonard J , Johnson James D , Kolic Jelena
Published: July 2024

Dietary carbohydrates raise blood glucose levels, and limiting carbohydrate intake improves glycemia in patients with type 2 diabetes. Low carbohydrate intake (< 25 g) allows the body to utilize fat as its primary fuel. As a consequence of increased fatty acid oxidation, the liver produces ketones to serve as an alternative energy source. β-Hydroxybutyrate (βHB) is the most abundant ketone. While βHB has a wide range of functions outside of the pancreas, its direct effects on islet cell function remain understudied. We examined human islet secretory response to acute racemic βHB treatment and observed increased insulin secretion at a low glucose concentration of 3 mM. Because βHB is a chiral molecule, existing as both R and S forms, we further studied insulin and glucagon secretion following acute treatment with individual βHB enantiomers in human and C57BL/6J mouse islets. We found that acute treatment with R-βHB increased insulin secretion and decreased glucagon secretion at physiological glucose concentrations in both human and mouse islets. Proteomic analysis of human islets treated with R-βHB over 72 hours showed altered abundance of proteins that may promote islet cell health and survival. Collectively, our data show that physiological concentrations of βHB influence hormone secretion and signaling within pancreatic islets.
Inspection key trends
Authors: Stephenson Jo
Published: July 2024

Ofsted's latest annual report highlights improving standards across children's social care but raises concerns about increasing numbers of children in unregistered provision, reports Jo Stephenson
Rowing boosts mental health and expands skills
Authors: Józwiakź Gabriella
Published: July 2024

Four-year programme saw young people from all backgrounds take opportunities to participate in rowing, improving theirimproving their health while developing life skills
Reforming school inspections
Authors: Knight Lord Jim
Published: July 2024

Chair of independent review of Ofsted says inspectorate should focus on helping schools improve
Steph Roberts-Bibby
Authors: Simpson Fiona
Published: July 2024

Fiona Simpson speaks to the new chief executive of the Youth Justice Board
UNLOCKING HELP FOR PRISONERS' CHILDREN
Authors: Ross Andy , Mapisa Karen
Published: July 2024

With the UK prison population at an all-time high, increasing numbers of children and young people have a parent in prison. Andy Ross explores the impact on families and how best to support them
Working with retailers
Authors: Waterfield Vivien
Published: July 2024

Charities can extend their reach by forming strategic partnerships with carefully selected retailers, generating vital extra funds and opening up opportunities for relationships that drive social change