bluuebunny/crossref_metadata_2025 huggingface dataset:
Translation and psychometric validation of the Chinese version of the meaningful and enjoyable activities scale for mild dementia
Authors: Chen Hongyu , Wang Yuanyuan , Zhang Minyi , Wang Ning , Hao Xixi , Xue Zhihan , Fang Kui , Liu Yan
Published: March 2023

Objectives

To translate 20-item Meaningful and Enjoyable Activities Scale into Chinese and evaluate its psychometric properties amongst Chinese with mild dementia.

Methods

A cross-sectional study of 450 people with mild dementia recruited from a memory disorders clinic was conducted with the C-MEAS. Raw data were randomly divided into two parts for exploratory factor analysis and confirmatory factor analysis, to evaluate the construct validity. Content validity and reliability were tested by content validity index and Cronbach’s α coefficients, respectively.

Results

Adaptation results showed that the Chinese version of the scale is adequate for linguistic and content validation. Confirmatory factor analysis indicated a significantly good fit for a three-factor model. Cronbach’s alpha coefficient was 0.84 for the overall scale.

Conclusion

The C-MEAS for people with mild dementia is a reliable and valid instrument with satisfactory psychometric properties. Future studies should recruit a more representative sample of people with mild dementia in China to verify the applicability of the scale.
Interactive robots for health in Europe: Technology readiness and adoption potential
Authors: Östlund Britt , Malvezzi Monica , Frennert Susanne , Funk Michael , Gonzalez-Vargas Jose , Baur Kilian , Alimisis Dimitris , Thorsteinsson Freygardur , Alonso-Cepeda Antonio , Fau Guillaume , Haufe Florian , Di Pardo Massimo , Moreno Juan C.
Published: March 2023

Introduction

Social robots are accompanied by high expectations of what they can bring to society and in the healthcare sector. So far, promising assumptions have been presented about how and where social robots are most relevant. We know that the industry has used robots for a long time, but what about social uptake outside industry, specifically, in the healthcare sector? This study discusses what trends are discernible, to better understand the gap between technology readiness and adoption of interactive robots in the welfare and health sectors in Europe.

Methods

An assessment of interactive robot applications at the upper levels of the Technology Readiness Level scale is combined with an assessment of adoption potential based on Rogers' theory of diffusion of innovation. Most robot solutions are dedicated to individual rehabilitation or frailty and stress. Fewer solutions are developed for managing welfare services or public healthcare.

Results

The results show that while robots are ready from the technological point of view, most of the applications had a low score for demand according to the stakeholders.

Discussion

To enhance social uptake, a more initiated discussion, and more studies on the connections between technology readiness and adoption and use are suggested. Applications being available to users does not mean they have an advantage over previous solutions. Acceptance of robots is also heavily dependent on the impact of regulations as part of the welfare and healthcare sectors in Europe.
Sedentary behavior, brain-derived neurotrophic factor and brain structure in midlife: A longitudinal brain MRI sub-study of the coronary artery risk development in young adults study
Authors: Zhang Xuan , Meirelles Osorio D. , Li Zhiguang , Yaffe Kristine , Bryan R. Nick , Qiu Chengxuan , Launer Lenore J.
Published: March 2023

Background

Brain-derived neurotrophic factor levels are higher in those who are physically active and lower in people with cognitive dysfunction. This study investigated whether brain-derived neurotrophic factor mediated or modified the association of sedentary time to MRI-estimated brain volumes in midlife.

Methods

Baseline (n = 612) and five-year follow-up (n = 418) data were drawn from the multicenter Coronary Artery Risk Development in Young Adults Brain MRI sub-study, including Black and White participants (aged 50.3 years, 51.6% females, 38.6% Black). Sedentary time (hours per day) was categorized into quartiles with low ≤ 4.3 (reference) and high > 8.4. Outcomes of the study were total brain, white matter, gray matter, hippocampal volumes, and white matter fractional anisotropy at baseline and 5-year percent change from baseline. The study used general linear regression models to examine the mediation and moderation effects of brain-derived neurotrophic factor (natural log transformed) on the associations of sedentary time to brain outcomes. The authors adjusted the regression model for age, sex, race, intracranial volume, education, and vascular factors.

Results

Cross-sectionally, baseline participants with the highest sedentary time had a lower total brain (−12.2 cc; 95%CI: −20.7, −3.7), gray matter (−7.8 cc; 95%CI: −14.3, −1.3), and hippocampal volume (−0.2 cc; 95%CI: −0.3, 0.0) compared with populations with the lowest sedentary time. The brain-derived neurotrophic factor levels did not mediate the associations between brain measures and sedentary time. Brain-derived neurotrophic factor was found to moderate associations of sedentary time to total brain and white matter volume such that the brain volume difference between high and low sedentary time decreased as brain-derived neurotrophic factor levels increased. Longitudinally, higher baseline brain-derived neurotrophic factor level was associated with less brain volume decline. The longitudinal associations did not differ by sedentary time, and brain-derived neurotrophic factor did not mediate or moderate the association of sedentary time to brain measure changes.

Conclusions

Higher brain-derived neurotrophic factor levels may buffer the negative effects of sedentary time on the brain.
A New Third-Order Family of Multiple Root-Findings Based on Exponential Fitted Curve
Authors: Kanwar Vinay , Cordero Alicia , Torregrosa Juan R. , Rajput Mithil , Behl Ramandeep
Published: March 2023

In this paper, we present a new third-order family of iterative methods in order to compute the multiple roots of nonlinear equations when the multiplicity (m≥1) is known in advance. There is a plethora of third-order point-to-point methods, available in the literature; but our methods are based on geometric derivation and converge to the required zero even though derivative becomes zero or close to zero in vicinity of the required zero. We use the exponential fitted curve and tangency conditions for the development of our schemes. Well-known Chebyshev, Halley, super-Halley and Chebyshev–Halley are the special members of our schemes for m=1. Complex dynamics techniques allows us to see the relation between the element of the family of iterative schemes and the wideness of the basins of attraction of the simple and multiple roots, on quadratic polynomials. Several applied problems are considered in order to demonstrate the performance of our methods and for comparison with the existing ones. Based on the numerical outcomes, we deduce that our methods illustrate better performance over the earlier methods even though in the case of multiple roots of high multiplicity.
Robust Liquid Level Control of Quadruple Tank System: A Nonlinear Model-Free Approach
Authors: Hashim Zahraa Sabah , Khani Halah I. , Azar Ahmad Taher , Khan Zafar Iqbal , Smait Drai Ahmed , Abdulwahab Abdulkareem , Zalzala Ali Mahdi , Jawad Anwar Ja’afar Mohamad , Ahmed Saim , Ibraheem Ibraheem Kasim , Najm Aws Abdulsalam , Fati Suliman Mohamed , Tounsi Mohamed , Mahlous Ahmed Redha
Published: March 2023

In this paper, two new versions of modified active disturbance rejection control (MADRC) are proposed to stabilize a nonlinear quadruple tank system and control the water levels of the lower two tanks in the presence of exogenous disturbances, parameter uncertainties, and parallel varying input set-points. The first proposed scheme is configured from the combination of a modified tracking differentiator (TD), modified super twisting sliding mode (STC-SM), and modified nonlinear extended state observer (NLESO), while the second proposed scheme is obtained by aggregating another modified TD, a modified nonlinear state error feedback (MNLSEF), and a fal-function-based ESO. The MADRC schemes with a nonlinear quadruple tank system are investigated by running simulations in the MATLAB/SIMULINK environment and several comparison experiments are conducted to validate the effectiveness of the proposed control schemes. Furthermore, the genetic algorithm (GA) is used as a tuning algorithm to parametrize the proposed MADRC schemes with the integral time absolute error (ITAE), integral square of the control signal (ISU), and integral absolute of the control signal (IAU) as an output performance index (OPI). Finally, the simulation results show the robustness of the proposed schemes with a noticeable reduction in the OPI.
Negotiating Autonomy: The Linkages between Intimate Partner Violence, Women’s Paid Work Status and Birth Outcomes
Authors: Ghatak Subhasree , Dutta Meghna
Published: March 2023

Maternal and child health is severely impacted by adverse birth outcomes leading to a public health concern. A whole host of socioeconomic factors are instrumental in determining birth outcomes. Importantly, there is an intricate relationship between women’s autonomy, the perpetration of intimate partner violence in households, women’s paid work status and their consequent impact on birth outcomes. Noting this, we ask how intimate partner violence and women’s work status interact and how women’s ‘autonomy’ is negotiated to mitigate adverse birth outcomes such as miscarriage, abortion, stillbirth, low birth weight and preterm birth. We use the nationally representative NFHS-5 data for India and use multiple correspondence analyses to create an index of women’s autonomy, and multinomial logistic regression has been used to determine the relation. Women’s working status in association with the perpetration of intimate partner violence contributes significantly to adverse birth outcomes. The study found that mitigation of adverse birth outcomes, which is necessary for bringing about improvements in maternal and child health, is contingent on a multiplicity of social factors, which requires redressal in association to ensure a reduction in adverse birth outcomes.
Uncertainty Quantification of Compressor Map Using the Monte Carlo Approach Accelerated by an Adjoint-Based Nonlinear Method
Authors: Xu Shenren , Zhang Qian , Wang Dingxi , Huang Xiuquan
Published: March 2023

Precise and inexpensive uncertainty quantification (UQ) is crucial for robust optimization of compressor blades and to control manufacturing tolerances. This study looks into the suitability of MC−adj−nonlinear, a nonlinear adjoint-based approach, to precisely and rapidly assess the performance discrepancies of a transonic compressor blade section, arising from geometric alterations, and building upon previous research. In order to assess the practicality and illustrate the benefits of the adjoint-based nonlinear approach, its proficiency and precision are gauged against two other methodologies, the adjoint-based linear approach (MC−adj−linear) and the high-fidelity nonlinear Computational Fluid Dynamics (MC−CFD) method. The MC−adj−nonlinear methodology exhibits impressive generalization capabilities. The MC−adj−nonlinear method offers a great balance between precision and time efficiency, since it is more precise than the MC−adj−linear method in both design and near-stall conditions, yet requires approximately a thirtieth of the time of the MC−CFD method. Finally, the MC−adj−nonlinear method was utilized to conduct fast UQ analyses of the section at four distinct speeds to quantify the performance uncertainty for the compressor map. It is found that aerodynamic performance is more sensitive to geometric deviations at high speeds than at low speeds. The impact of the geometric deviations is generally detrimental to the mean efficiency.
Decarbonised Future Regional Airport Infrastructure
Authors: Meindl Markus , de Ruiter Cor , Marciello Valerio , Stasio Mario Di , Hilpert Florian , Ruocco Manuela , Nicolosi Fabrizio , Thonemann Nils , Saavedra-Rubio Karen , Locqueville Louis , Laurent Alexis , Maerz Martin
Published: March 2023

Sustainability and, especially, emission reductions are significant challenges for airports currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040) and long (2050) time horizon are proposed. This analysis includes estimating the future energy demand per day, month and year. In addition, the current flight plan based on conventional aircraft is adapted to the needs of a 50-PAX regional aircraft. Thus, this article provides an overview of the energy demand of a regional airport, divided into individual time horizons.
Heavy Metals Influence on the Bacterial Community of Soils: A Review
Authors: Sazykin Ivan , Khmelevtsova Ludmila , Azhogina Tatiana , Sazykina Marina
Published: March 2023

The increasing rate of natural resource use leads to an increase in the anthropogenic load on the soil. As the result of industrial, metallurgical and mining activities, excessive amounts of heavy metals (HMs) enter the soil. In addition, they can be introduced with waste and drainage water from various enterprises. Accumulating in the soil, HMs can negatively affect the soil bacterial community, which is one of the main factors of its “health”. Molecular genetic methods based on shotgun sequencing or metabarcoding of standard DNA sequences (for example, the 16S rRNA gene for bacteria) are the modern ways to assess the bacterial diversity of soils. This review presents the results of modern studies on the effect of HMs on the soil bacterial communities, using metagenomic methods. Based on the analysis of publications over the past two decades, a generally negative effect of pollutants on the taxonomic composition and diversity of bacterial communities has been shown. The influence of factors modulating the toxicity of metals and metalloids was noted: the amount and composition of salts, soil pH, ecosystem type, rhizosphere presence and other soil properties. In this paper, promising directions of research are outlined.
Design and Test of Sensor for Monitoring Corn Cleaning Loss
Authors: Wei Dexin , Wu Chongyou , Jiang Lan , Wang Gang , Chen Hui
Published: March 2023

At present, Chinese corn grain harvesters lack cleaning loss monitoring. Cleaning parameters cannot be automatically adjusted, and the loss rate is high. In view of the above problems, a cleaning loss monitoring sensor is designed, composed of a metal impact plate, piezoelectric ceramic and signal processing circuit. The factors affecting the characteristics of vibration signals are analyzed from the material, size and other aspects. The sensitive plate is composed of a 304 stainless steel impact plate and piezoelectric ceramic. The sensitive plate can convert the vibration signal of the impact plate into a voltage signal, and the output voltage range can reach ±3 V or more. The signal generated by the collision of corn kernel and damaged corn cob with the sensitive plate was analyzed.It was found that the frequency domain range of corn grains was wider, with signals above 6 kHz, but broken corncobs did not have such signals. Based on the frequency distribution, a signal processing circuit is designed, which consists of high-pass filter circuit, an envelope detection circuit, and a voltage comparison circuit. The circuit can convert analog signals into pulse signals, which facilitates the counting process by the microprocessor. In order to obtain the monitoring accuracy and installation parameters of the integrated corn cleaning loss monitoring sensor, a Central Composite Design was carried out with the installation height and angle of the sensitive plate as the test factors and monitoring accuracy as the test index. Based on the test results and field test conditions, a regression model was established to determine the optimal installation parameters: the installation angle of the sensitive plate is 30°, and the installation height is 30 cm. At this stage, the accuracy of the sensor monitoring corn grains was 92.82%, and the accuracy of monitoring the mixture of corn grains and broken corncobs was 90.07%. The verification test shows that the monitoring accuracy can reach more than 94% after the sensor is debugged. This research can provide a reference for the design of corn cleaning loss monitoring devices.
Essential Oils and Chitosan Applications to Protect Apples against Postharvest Diseases and to Extend Shelf Life
Authors: Soppelsa Sebastian , Van Hemelrijck Wendy , Bylemans Dany , Andreotti Carlo
Published: March 2023

Apple fruits are susceptible to pathogenic fungi such as Botrytis cinerea and Penicillium expansum which are responsible for significant pre- and postharvest fruit losses. Given the strong restriction in the use of synthetic agrochemicals, especially during the postharvest phase, alternatives are currently sought for a more sustainable management of apple storage. The antifungal activity of thyme, clove, anise, camphor, and juniper essential oils (EOs) was evaluated with in vitro tests and the minimum inhibitory concentration (MIC) was determined. Thyme and anise EOs showed a MIC value of 0.5 and 1 mL L−1 against B. cinerea, respectively. A MIC of 0.5 mL L−1 was found for clove EO against P. expansum. Camphor and juniper EOs were found to be less effective. Although in those experiments the antifungal activity of EOs was proven, when EOs were applied in in vivo conditions they did not demonstrate the same effectiveness. In order to preserve EO inhibitory performances, edible coatings combining polysaccharide matrices (chitosan, arabic gum, and xanthan gum) with EOs were tested. After considering consistency, uniform coverage of the fruit surface, and antifungal properties of the matrix, chitosan was identified as the most suitable component for EO encapsulation. Treatments with chitosan in combination with thyme EO on apple fruits (cv. ‘Braeburn’) showed inhibitory effects on infection caused by B. cinerea (−48% of infected wounds compared to untreated). Similarly, clove EO combined with chitosan reduced the Penicillium infections in apple cv. ‘Golden Delicious’ by 62%. These results therefore suggest the effectiveness of the use of EOs encapsulated with chitosan for the control of postharvest diseases of apple fruits during storage.
Development of a Mobile Open-Circuit Respiration Head Hood System for Measuring Gas Exchange in Camelids in the Andean Plateau
Authors: Rios Rado Wilfredo Manuel , Chipa Guillen Paola Katherine , Huamán Borda Dione , Vélez Marroquín Víctor , Gere José Ignacio , Antezana Julián Walter Orestes , Fernández Carlos
Published: March 2023

Peru has the largest inventory of alpacas worldwide. Despite their importance as a source of net income for rural communities living at the Andean Plateau, data on energy requirements and methane (CH4) emissions for alpacas are particularly lacking. In 2019, the International Panel on Climate Change (IPCC; 2006, and Refinement 2019) outlined methods for estimating CH4 emissions from enteric fermentation and no methane (CH4) conversion factors were reported for camelids. IPCC has since updated its guidelines for estimating CH4 emissions from the enteric fermentation of livestock at a national scale. For greenhouse gas (GHG) inventory purposes, conversion factors were developed for ruminants but not for domestic South American camelids (SAC), with this category including alpacas. A mobile open-circuit respirometry system (head hood) for the rapid determination of CH4 and CO2 production, O2 consumption, and thereafter, heat production (HP) for camelids was built and validated. In addition, an experimental test with eight alpacas was conducted for validation purposes. The average HP measured by indirect calorimetry (respiratory quotient (RQ) method) was close to the average HP determined from the carbon–nitrogen balance (CN method); 402 kJ/kg BW0.75 and 398 kJ/kg BW0.75, respectively. Fasting HP was determined by the RQ method and 250 kJ/kg BW0.75 was obtained. The metabolizable energy requirement for maintenance (MEm) was calculated to be 323 kJ/kg BW0.75 with an efficiency of energy utilization of 77%. When intake was adjusted to zero energy retention by linear regression, the MEm requirement increased to 369 kJ/kg BW0.75 and the efficiency decreased up to 68%. The CH4 conversion factor (Ym) was 5.5% on average. Further research is required to gain a better understanding of the energy requirements and CH4 emissions of alpacas in conditions of the Andean Plateau and to quantify them with greater accuracy.
Chicken Hypothalamic and Ovarian DNA Methylome Alteration in Response to Forced Molting
Authors: Zhang Tongyu , Li Chengfeng , Deng Jianwen , Jia Yaxiong , Qu Lujiang , Ning Zhonghua
Published: March 2023

Epigenetic modifications play an important role in regulating animal adaptation to external stress. To explore how DNA methylation regulates the expression levels of related genes during forced molting (FM) of laying hens, the hypothalamus and ovary tissues were analyzed at five periods using Whole-Genome Bisulfite Sequencing. The results show that methylation levels fluctuated differently in the exon, intron, 5′UTR, 3′UTR, promoter, and intergenic regions of the genome during FM. In addition, 16 differentially methylated genes (DMGs) regulating cell aging, immunity, and development were identified in the two reversible processes of starvation and redevelopment during FM. Comparing DMGs with differentially expressed genes (DEGs) obtained in the same periods, five hypermethylated DMGs (DSTYK, NKTR, SMOC1, SCAMP3, and ATOH8) that inhibited the expression of DEGs were found. Therefore, DMGs epigenetically modify the DEGs during the FM process of chickens, leading to the rapid closure and restart of their reproductive function and a re-increase in the egg-laying rate. Therefore, this study further confirmed that epigenetic modifications could regulate gene expression during FM and provides theoretical support for the subsequent optimization of FM technology.
Enhancing Digestibility of Chlorella vulgaris Biomass in Monogastric Diets: Strategies and Insights
Authors: Spínola Maria P. , Costa Mónica M. , Prates José A. M.
Published: March 2023

Microalgae, such as Chlorella vulgaris (CV), have been identified as promising animal feed sources due to their high content of essential nutrients, including proteins, total lipids, n-3 polyunsaturated fatty acids, and pigments. This study aimed to review the digestibility, bioaccessibility, and bioavailability of nutrients from CV biomass, and to analyse strategies to enhance their digestibility in monogastric animal diets. The study conducted a systematic review of the literature from databases such as PubMed, Scopus, Google Scholar, and Web of Science, up until the end of January 2023. The results of adding CV to poultry and swine diets were diverse and depended on a number of variables. However, pre-treatments applied to CV biomass improved nutrient digestibility and accessibility. CV biomass, produced in a cost-effective manner, has the potential to serve as a supplement or substitute for expensive feed ingredients and improve animal health, physiology, and immune status. Variations in results may be due to differences in microalgal strain, cultivation conditions, and dietary inclusion levels, among other factors. This study provides new insights and perspectives into the utilization of CV biomass in animal diets, highlighting its potential as a valuable ingredient to improve nutrient utilization.
Dexmedetomidine Has Differential Effects on the Contractility of Equine Jejunal Smooth Muscle Layers In Vitro
Authors: Verhaar Nicole , Hoppe Susanne , Grages Anna Marei , Hansen Kathrin , Neudeck Stephan , Kästner Sabine , Mazzuoli-Weber Gemma
Published: March 2023

α2 agonists are frequently used in horses with colic, even though they have been shown to inhibit gastrointestinal motility. The aim of this study was to determine the effect of dexmedetomidine on small intestinal in vitro contractility during different phases of ischaemia. Experimental segmental jejunal ischaemia was induced in 12 horses under general anaesthesia, and intestinal samples were taken pre-ischaemia and following ischaemia and reperfusion. Spontaneous and electrically evoked contractile activity of the circular and longitudinal smooth muscles were determined in each sample with and without the addition of dexmedetomidine. During a second experiment, tetrodotoxin was added to determine if the effect was neurogenic. We found that the circular smooth muscle (CSM) contractility was not affected by ischaemia, whereas the longitudinal smooth muscle (LSM) showed an increase in both spontaneous and induced contractile activity. The addition of dexmedetomidine caused a decrease in the spontaneous contractile activity of CSM, but an increase in that of LSM, which was not mediated by the enteric nervous system. During ischaemia, dexmedetomidine also mildly increased the electrically induced contractile activity in LSM. These results may indicate a stimulatory effect of dexmedetomidine on small intestinal contractility. However, the influence of dexmedetomidine administration on intestinal motility in vivo needs to be further investigated.
The Impact of Beehive Proximity, Human Activity and Agricultural Intensity on Diptera Diversity in a Mediterranean Mosaic of Agroecosystems, with a Focus on Pest Species
Authors: Sladonja Barbara , Tlak Gajger Ivana , Uzelac Mirela , Poljuha Danijela , Garau Clara , Landeka Nediljko , Barták Miroslav , Bacaro Giovanni
Published: March 2023

Diptera, with their participation in pollination, significantly contribute to the maintenance of plant diversity, and they also have great potential for assessing habitat health and preserving it. A decline in their abundance and diversity has been recorded worldwide as a consequence of biotic, abiotic, and anthropic alterations. In addition to pollinators, these orders include agricultural and forestry pests, which are a threat to both cultivated and wild plants that are very important to the economy. Many pests have escaped from their native areas, and it is important to monitor their spread to implement sustainable means of control. Our study provides baseline information on Diptera and Vespidae diversity in the Mediterranean mosaic of agroecosystems, giving information on the importance of human influence on insect diversity. We carried out an insect inventory in Istria, Croatia, using a set of traps placed in the proximity of beehives. This study was also important in determining the presence of pests and newly introduced species. A total of 94 species from 24 families were recorded—7 important agricultural pests of Diptera and 17 new records for Croatia. The correlation between species diversity and environmental and anthropogenic factors leads to the conclusion that total insect species richness, pest species richness, and the first findings depend on human activities. The number of honeybee colonies negatively correlated with species richness, while anthropic influence positively affected total and pest species richness.
How Do Dogs Behave When Presented with Situations of Different Emotional Valences?
Authors: Souza Paulo , Guo Kun , Mills Daniel S. , Resende Briseida , Albuquerque Natalia
Published: March 2023

Dogs are good models for studying behaviour and cognition as they have complex social capabilities. In the current study, we observed how human emotional valences (positive, neutral and negative) affected aspects of dogs’ behaviour. We expected that dogs would exhibit more approaching behaviours in the positive condition and more signs of avoidance in the negative one. We analysed videos of 70 adult pet dogs of various breeds taken from an experiment in which one of two actors expressed an emotion and dogs could freely explore the environment for 30 s. Our results show that dogs exhibit differential behaviour when presented with different emotional valences. Two behaviours arose that might be linked to a reciprocal positive emotional state in dogs: tail raised between 90° and 180° and physical contact during sniffing. These behaviours are associated with an active search for information. In the positive conditions, dogs were more willing to explore the social environment and gather information from the actors.
HV1 mtDNA Reveals the High Genetic Diversity and the Ancient Origin of Vietnamese Dogs
Authors: Thai Quan Ke , Nguyen Thanh-Cong , Le Cong-Trieu , Chung Anh-Dung , Nguyen Tran Minh-Ly , Tran Quoc-Dung , Savolainen Peter , Quan Quoc-Dang , Tran Dai-Long , Tran Hoang-Dung
Published: March 2023

In this study, samples from 429 dog individuals across three main regions of Vietnam (Southern Vietnam (SVN), Central Vietnam (CVN), and Northern Vietnam (NVN)) were collected to analyze the 582 bp region mtDNA HVI, so as to study the genetic diversity and to screen the rare haplotype E in the Vietnamese village dog population. Nine new haplotypes A, two new haplotypes B, and three haplotypes C were unique to Vietnam dogs, in which the new haplotypes An3, An7, Cn1, and Cn3 concerned mutations at new polymorphism sites (15,517, 15,505, 15,479, and 15,933, respectively) which have not been previously reported. The detection of haplotypes A9 and A29, and the appearance of haplotype A200 in the two individual dogs sampled support that the Southeast Asian dog is the ancestor of today’s Australian dingo and Polynesian dog. The two rare haplotypes E (E1 and E4) were reconfirmed in Vietnamese dogs and discussed. This study also contributes to strengthening the theory of domestication of dogs to the south of the Yangtze River and the Southeast Asian origin of the dingo.
Not All Puppies and Sunshine: How Dog Keepers Cope with Dog-Related Problems in Dutch Society
Authors: Ophorst Susan , Aarts Noelle , Bovenkerk Bernice , Hopster Hans
Published: March 2023

Zoonoses, biting incidents, hereditary problems, and other issues can threaten the welfare of both humans and dogs. The Dutch government and animal welfare organizations seem to have little effect in their campaigns to influence the behavior of (potential) dog keepers, who can experience dissonance when faced with these campaigns and use coping strategies to relieve the dissonance instead of changing their behavior. In this study, in focus group discussions, dog keepers with pedigree dogs, high-risk dogs, foreign shelter dogs, and dogs purchased at puppy farms shared their experiences with opinions on dogs and were confronted with negative opinions on their dogs. The data were analyzed using a coping strategies framework. Most coping strategies were found in all groups, but were used in response to different dilemmas, with different manifestations. These differences should be kept in mind when behavior change in dog keepers is opportune. Special attention should be given to differentiating target groups, as use of the detachment coping strategy suggests that boundaries might be set differently than expected. Broad attention on problems with and for dogs can address perceived dissonance and prompt behavior change. In dialogue with dog keepers, in influential campaigns and in policy formulation, the chances of success are greater if initiators are aware of the strategies that they may encounter.
The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk
Authors: Sipahi Nisa , Kaya Ertugrul , Çelik Cansu , Pınar Orhan
Published: March 2023

Staphylococci is an opportunistic bacterial population that is permanent in the normal flora of milk and poses a serious threat to animal and human health with some virulence factors and antibiotic-resistance genes. This study was aimed at identifying staphylococcal species isolated from raw milk and to determine hemolysis, biofilm, coagulase activities, and beta-lactam resistance. The raw milk samples were collected from the Düzce (Türkiye) region, and the study data represent a first for this region. The characterization of the bacteria was performed with MALDI-TOF MS and 16S rRNA sequence analysis. The presence of coa, icaB, blaZ, and mecA was investigated with PCR. A nitrocefin chromogenic assay was used for beta-lactamase screening. In this context, 84 staphylococci were isolated from 10 different species, and the dominant species was determined as S. aureus (32.14%). Although 32.14% of all staphylococci were positive for beta hemolysis, the icaB gene was found in 57.14%, coa in 46.42%, mecA in 15.47%, and blaZ in 8.33%. As a result, Staphylococcus spp. strains that were isolated from raw milk in this study contained some virulence factors at a high level, but also contained a relatively low level of beta-lactam resistance genes. However, considering the animal–environment–human interaction, it is considered that the current situation must be monitored constantly in terms of resistance concerns. It must not be forgotten that the development of resistance is in constant change among bacteria.
Clinical Impact of Staphylococcus aureus Skin and Soft Tissue Infections
Authors: Linz Matthew S. , Mattappallil Arun , Finkel Diana , Parker Dane
Published: March 2023

The pathogenic bacterium Staphylococcus aureus is the most common pathogen isolated in skin-and-soft-tissue infections (SSTIs) in the United States. Most S. aureus SSTIs are caused by the epidemic clone USA300 in the USA. These infections can be serious; in 2019, SSTIs with S. aureus were associated with an all-cause, age-standardized mortality rate of 0.5 globally. Clinical presentations of S. aureus SSTIs vary from superficial infections with local symptoms to monomicrobial necrotizing fasciitis, which can cause systemic manifestations and may lead to serious complications or death. In order to cause skin infections, S. aureus employs a host of virulence factors including cytolytic proteins, superantigenic factors, cell wall-anchored proteins, and molecules used for immune evasion. The immune response to S. aureus SSTIs involves initial responders such as keratinocytes and neutrophils, which are supported by dendritic cells and T-lymphocytes later during infection. Treatment for S. aureus SSTIs is usually oral therapy, with parenteral therapy reserved for severe presentations; it ranges from cephalosporins and penicillin agents such as oxacillin, which is generally used for methicillin-sensitive S. aureus (MSSA), to vancomycin for methicillin-resistant S. aureus (MRSA). Treatment challenges include adverse effects, risk for Clostridioides difficile infection, and potential for antibiotic resistance.
In Vitro Antimicrobial Activity of N-Acetylcysteine against Pathogens Most Commonly Associated with Infectious Keratitis in Dogs and Cats
Authors: Walter Hanna , Verspohl Jutta , Meißner Jessica , Oltmanns Hilke , Geks Anna Karoline , Busse Claudia
Published: March 2023

To determine the in vitro antimicrobial activity of N-acetylcysteine (NAC) against common pathogens associated with infectious keratitis in dogs and cats, clinical isolates of Staphylococcus (S.) pseudintermedius (n = 20), Streptococcus (St.) canis (n = 10) and Pseudomonas (P.) aeruginosa (n = 7) of canine and feline infectious ulcerative keratitis and a quality control strain (P. aeruginosa DSM 19880) were tested. The minimal inhibitory concentration (MIC) of NAC concentrations was determined using microdilution methodology. For S. pseudintermedius and P. aeruginosa, NAC concentrations in the range of 1.56 mg/mL (0.156%) to 100 mg/mL (10%), and for St. canis, concentrations ranging from 0.195 mg/mL (0.0195%) to 6.25 mg/mL (0.625%) were tested. For S. pseudintermedius, the MIC was 3.12 mg/mL (0.312%) for all tested isolates. For P. aeruginosa isolates and the quality control strain, the MIC ranged from 3.12 mg/mL (0.312%) to 6.25 mg/mL (0.625%). For St. canis, the MIC ranged from 1.56 mg/mL (0.156%) to 3.12 mg/mL (0.312%). NAC has an in vitro antimicrobial activity against three bacterial species commonly found in infectious keratitis in dogs and cats and therefore may be a promising alternative or adjuvant to topical antibiotics. The results warrant a clinical pilot study to assess the potential of NAC to reduce or replace the use of topical antibiotics in line with the One Health approach.
The Extracellular Matrix Vitalizer RATM Increased Skin Elasticity by Modulating Mitochondrial Function in Aged Animal Skin
Authors: Byun Kyung-A , Oh Seyeon , Batsukh Sosorburam , Kim Min Jeong , Lee Je Hyuk , Park Hyun Jun , Chung Moon Suk , Son Kuk Hui , Byun Kyunghee
Published: March 2023

Oxidative stress-induced cellular senescence and mitochondrial dysfunction result in skin aging by increasing ECM levels-degrading proteins such as MMPs, and decreasing collagen synthesis. MMPs also destroy the basement membrane, which is involved in skin elasticity. The extracellular matrix vitalizer RATM (RA) contains various antioxidants and sodium hyaluronate, which lead to skin rejuvenation. We evaluated whether RA decreases oxidative stress and mitochondrial dysfunction, eventually increasing skin elasticity in aged animals. Oxidative stress was assessed by assaying NADPH oxidase activity, which is involved in ROS generation, and the expression of SOD, which removes ROS. NADPH oxidase activity was increased in aged skin and decreased by RA injection. SOD expression was decreased in aged skin and increased by RA injection. Damage to mitochondrial DNA and mitochondrial fusion markers was increased in aged skin and decreased by RA. The levels of mitochondrial biogenesis markers and fission markers were decreased in aged skin and increased by RA. The levels of NF-κB/AP-1 and MMP1/2/3/9 were increased in aged skin and decreased by RA. The levels of TGF-β, CTGF, and collagen I/III were decreased in aged skin and increased by RA. The expression of laminin and nidogen and basement membrane density were decreased in aged skin and increased by RA. RA increased collagen fiber accumulation and elasticity in aged skin. In conclusion, RA improves skin rejuvenation by decreasing oxidative stress and mitochondrial dysfunction in aged skin.
In Silico and In Vitro Study of Antioxidant Potential of Urolithins
Authors: Marchese Emanuela , Orlandi Valentina , Turrini Federica , Romeo Isabella , Boggia Raffaella , Alcaro Stefano , Costa Giosuè
Published: March 2023

In this work, quantum chemical calculations based on density functional theory (DFT) were performed to predict the antioxidant potential of four bioactive gut microbiota metabolites of the natural polyphenols ellagitannins (ETs) and ellagic acid (EA), also known as urolithins (UROs). In order to evaluate their ability to counter the effect of oxidative stress caused by reactive oxygen species (ROS), such as the hydroperoxyl radical (•OOH), different reaction mechanisms were investigated, considering water and lipid-like environments. Through our in silico results, it emerged that at physiological pH, the scavenging activity of all urolithins, except urolithin B, are higher than that of trolox and other potent antioxidants existing in nature, such as EA, α-mangostin, allicin, caffeine and melatonin. These findings were confirmed by experimental assays.
Thioredoxin-2 Regulates SqrR-Mediated Polysulfide-Responsive Transcription via Reduction of a Polysulfide Link in SqrR
Authors: Shimizu Takayuki , Hashimoto Masaru , Masuda Tatsuru
Published: March 2023

Polysulfide plays an essential role in controlling various physiological activities in almost all organisms. We recently investigated the impact of polysulfide metabolic enzymes on the temporal dynamics of cellular polysulfide speciation and transcriptional regulation by the polysulfide-responsive transcription factor SqrR in Rhodobacter capsulatus. However, how the polysulfidation of thiol groups in SqrR is reduced remains unclear. In the present study, we examined the reduction of polysulfidated thiol residues by the thioredoxin system. TrxC interacted with SqrR in vitro and reduced the polysulfide crosslink between two cysteine residues in SqrR. Furthermore, we found that exogenous sulfide-induced SqrR de-repression during longer culture times is maintained upon disruption of the trxC gene. These results establish a novel signaling pathway in SqrR-mediated polysulfide-induced transcription, by which thioredoxin-2 restores SqrR to a transcriptionally repressed state via the reduction of polysulfidated thiol residues.
Ryanodine Receptor Mediated Calcium Release Contributes to Ferroptosis Induced in Primary Hippocampal Neurons by GPX4 Inhibition
Authors: Gleitze Silvia , Ramírez Omar A. , Vega-Vásquez Ignacio , Yan Jing , Lobos Pedro , Bading Hilmar , Núñez Marco T. , Paula-Lima Andrea , Hidalgo Cecilia
Published: March 2023

Ferroptosis, a newly described form of regulated cell death, is characterized by the iron-dependent accumulation of lipid peroxides, glutathione depletion, mitochondrial alterations, and enhanced lipoxygenase activity. Inhibition of glutathione peroxidase 4 (GPX4), a key intracellular antioxidant regulator, promotes ferroptosis in different cell types. Scant information is available on GPX4-induced ferroptosis in hippocampal neurons. Moreover, the role of calcium (Ca2+) signaling in ferroptosis remains elusive. Here, we report that RSL3, a selective inhibitor of GPX4, caused dendritic damage, lipid peroxidation, and induced cell death in rat primary hippocampal neurons. Previous incubation with the ferroptosis inhibitors deferoxamine or ferrostatin-1 reduced these effects. Likewise, preincubation with micromolar concentrations of ryanodine, which prevent Ca2+ release mediated by Ryanodine Receptor (RyR) channels, partially protected against RSL3-induced cell death. Incubation with RSL3 for 24 h suppressed the cytoplasmic Ca2+ concentration increase induced by the RyR agonist caffeine or by the SERCA inhibitor thapsigargin and reduced hippocampal RyR2 protein content. The present results add to the current understanding of ferroptosis-induced neuronal cell death in the hippocampus and provide new information both on the role of RyR-mediated Ca2+ signals on this process and on the effects of GPX4 inhibition on endoplasmic reticulum calcium content.
Modeling and Analysis of Drill String–Casing Collision under the Influence of Inviscid Fluid Forces
Authors: Tchomeni Kouejou Bernard Xavier , Sozinando Desejo Filipeson , Anyika Alugongo Alfayo
Published: March 2023

The current study aims to study the drill string–casing system operating in an inviscid fluid under imbalanced and rubbing damage types. The Navier–Stokes equations were linearized to establish the hydrodynamic forces surrounding the drill string and resulted in a five-dimensional system of nonlinear differential equations. To ensure the accurate acquisition of friction characteristics in a fluid medium, a nonlinear wavelet synchronized transform (NWSST) technique was enhanced based on the denoised wavelet hard thresholding algorithm to extract the features of the rubbing system. The developed model was verified through various test conditions, and the extracted data tests show that the frictional impact proves sufficient to modify the dynamic behavior of the drill string throughout the energy concentration with a slight shift above and below the resonant frequency. It was shown by simulation that the vibration of the submerged drill string system potentially enhanced highly undesirable hidden vibrational frequencies that led to a disturbed and chaotic 3D orbit pattern vibrational response. The experimental results show how vibration analysis combined with the synchrosqueezed technique can identify the condition of the drill string system even under harsh operating conditions and demonstrate that fluid enables the drill string system to rotate with minimum friction.
Improved Low-Depth SHA3 Quantum Circuit for Fault-Tolerant Quantum Computers
Authors: Song Gyeongju , Jang Kyungbae , Seo Hwajeong
Published: March 2023

To build a secure cryptography system in the post-quantum era, one must find the minimum security parameters against quantum attacks by estimating the quantum resources of a fault-tolerant quantum computer. In a fault-tolerant quantum computer, errors must reach an acceptable level for practical uses according to error detection and error correction processes. However, these processes utilize additional quantum resources. As the depth of the quantum circuit increases, the computation time per qubit increases together with the processing errors. Therefore, in terms of errors in quantum circuits, it is a fundamental requirement to reduce the depth by trading off the number of qubits. This paper proposes novel low-depth SHA3 quantum circuit implementations for fault-tolerant quantum computers to reduce errors. The proposed SHA3 quantum circuit was implemented with the aim of optimizing the quantum circuit depth through a trade-off between the number of qubits, the quantum gate, and the quantum depth in each function. Compared to other state-of-art techniques, the proposed method achieved T-depth and full-depth reductions of 30.3% and 80.05%, respectively. We believe that this work will contribute to the establishment of minimum security parameters for SHA3 in the quantum era.
Challenges, Issues, and Recommendations for Blockchain- and Cloud-Based Automotive Insurance Systems
Authors: Mateen Abdul , Khalid Adia , Lee Sihyung , Nam Seung Yeob
Published: March 2023

Despite the rapid expansion in the insurance industry, many issues remain unresolved and may require immediate action. As the insurance sector continues to evolve with the development of new technologies, it faces more challenges, especially related to data security and fraud. The fraud-prevention data and tactics presently used by insurance firms are outdated and ineffective. Additionally, insurance firms have traditionally handled the settlement of all consumer claims through lengthy manual processes. These manual processes need to be changed to provide opportunities for insurance businesses to grow. In the case of vehicles, the information obtained from an automobile data recorder can be used as evidence. Data from automated vehicles are critical because they can help the police, law enforcement agencies, and insurance companies to reconstruct the events leading up to a collision. Insurance companies require the forensic analysis of accident videos, which is a time-consuming process and involves a large amount of storage. Due to hardware limitations and associated costs, the current standalone (and often dedicated) computing infrastructures used for this purpose are quite limited. Previous research focused on simple video analysis tasks within cloud computing and blockchain technology. The requirements for a large-scale auto-insurance system are quite high and need more thorough investigation. In this paper, a review of the contribution of recent approaches to storing accidental data in cloud computing using blockchain is provided. We focused on the latest cloud and blockchain studies related to auto-insurance along with the related issues and challenges. Some useful solutions and recommendations are provided to address the identified issues and challenges in the cloud-based and blockchain-based auto-insurance sector.