bluuebunny/crossref_metadata_2025 huggingface dataset:
UJI AKTIVITAS ANTIBAKTERI PADA MACAM MACAM MADU PADA BAKTERI ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS DENGAN METODE DIFUSI AGAR DAN DILUSI CAIR
Authors: Putri Siti Aisyah Ratna , Susanto Haryanto , Tambun Sabrina Handayani , Oktiarso Teguh
Published: March 2022

This study is an experimental laboratory, conducted to get the antibacterial activity in the types of multiflora honey, randu honey, mente honey, rambutan honey, sono honey, acacia honey, rubber honey. In this study, preliminary tests, phytochemical screening, and antibacterial tests have been carrying out to determine the Minimum Inhibitory Level (MIC) and Minimum Killing Rate (KBM) at concentrations of 20, 25, 50, and 100%. Phytochemical test results it's known that each honey contains positive alkaloids, flavonoids, glycosides, and saponins. Antibacterial test results showed that each honey's positively had antibacterial activity. MIC and KBM obtained showed that each honey can inhibit the growth of bacteria, but only rambutan honey in Escherichia coli bacteria cannot kill bacteria.

Keywords: antibacterial, honey, MIC, KBM, E.coli, S.aureus, disc diffusion, dilution
ANALYSIS OF CREW APPS APPLICATION TECHNOLOGY ACCEPTANCE AT PT PATRIA MARITIME LINE UNITED TRACTOR WITH TECHNOLOGY ACCEPTANCE MODEL 2 METHOD
Authors: Amelia Yuri , Prasetya Hendro Poerbo
Published: March 2022

Penerapan teknologi informasi disebuah perusahaan sudah banyak dilakukan di berbagai sektor perusahaan. Sebagai beberapa contohnya adalah dengan penerapan teknologiinformasi dibangun suatu website atau aplikasi guna memberikan kemudahan dan efisiensi dalam pekerjaan. Penerapan teknologi informasi ini dinilai sebagai salah satu cara untuk membangun perusahaan lebih baik agar tidak tertinggal dan mencapai tujuan yang sesuai. Penerapan teknologi informasi di sebuah perusahaan sudah banyak dilakukan di berbagai sektor perusahaan. Sebagai beberapa contohnya adalah dengan penerapan teknologi informasi dibangun suatu website atau aplikasi guna memberikan kemudahan dan efisiensi dalampekerjaan.
Penelitian ini dilakukan dengan sebutan metode yang bernama Technology Acceptance Model 2 yang mana metode tersebut memiliki beberapa konstruk yang bisa digunakan untuk menilai pendapat dan penerimaan dari pegawai head office dan crew kapal.
Metode tersebut juga nantinya bisa menunjukkan hubungan dan pengaruh antar variabel. Berdasarkan hasil penelitian diketahui memang faktor-faktor yang dipilih pada pengajuan hipotesis saling berhubungan dan berpengaruh signifikan positif terhadap masing-masing variabel. Sebagai contoh pula bahwa responden sangat terbantu dengan adanya aplikasi crew apps yang memiliki aplikasi user friendly, dan efisien.
PERANCANGAN BUKU TUTORIAL MENGGAMBAR ANATOMI MANUSIA GAYA SHOUNEN MANGA BAGI REMAJA
Authors: Fatimah Raissa , Nirwana Aditya , Pramudya Bintang
Published: March 2022

Informasi ditemukan dalam kehidupan sehari-hari. Dimana informasi dapat dikaitkan dengan munculnya keperluan untuk menambah wawasan supaya dapat memvalidasi sebuah informasi. Sehingga usaha yang dilakukan seseorang untuk mencari informasi dapat dinamakan dengan proses belajar. Metode proses belajar dapat dilakukan dengan murid didampingi oleh tutor yang menguasai suatu materi dengan suatu jadwal tertentu. Hal ini disebut dengan metode belajar tradisional, namun seiring berkembangnya waktu proses belajar dapat dilakukan secara otodidak dengan menggunakan media tutorial yang dilengkapi ilustrasi. Salah satunya adalah menggambar anatomi manusia dengan gaya Shounen Manga yang berkembang seiring kejenuhan pada target audience. Sehingga diperlukan perancangan buku tutorial menggambar anatomi Shounen Manga yang mampu meningkatkan minat dan mempermudah pembelajaran bagi remaja. Dalam penelitian ini dilakukan metode secara kuantitatif dan kualitatif dengan pengumpulan data berupa studi pustaka, kuesioner, dan observasi. Lalu didukung oleh media seperti buku sketsa, cover pen tablet, cat air, dan akun Instagram.
Designing Motorcycle Security System Using E-KTP Sensor and SMS Gateway
Authors: Maulidin Juani Khuswara , Hiswara Abrar , Sari Rafika
Published: March 2022

Motorcycle demand is increasing as well as the cases of vehicle theft in Indonesia. Not only increase theft cases, number of new modes of theft of the motorcycle is also increase. According to the Statistics Supervisory Agency (BPS) data from 2015 to 2018, the data on the number of motorcycles is increasing, starting every year around 126,508,776 motorcycles are sold in Indonesia. In Indonesia, it is estimated that hundreds of motorbikes are stolen every month. How is the feasibility of motorcycle safety devices and the accuracy of the RFID module. Creating a motorcycle security system using a microcontroller-based RFID-RC522 module that can be read accurately and can be monitored in real time using received SMS and test the feasibility of a motorcycle safety device that can be monitored via cellphone. Making this tool using the Prototype method. The prototype is a development method from the previous system. The results of this research are in accordance with the analysis and design that has been made to get the result of an E-KTP-based motorcycle safety device to increase the security of personal items because this device can activate and deactivate motorcycles by using SMS. This tool can also help the community in maintaining security around the surrounding environment so that they can add a double security system to motorcycle vehicles.
Android-Based Shortest Path Finding Using A-Star (A*) Algorithm in Bekasi City
Authors: Herlawati Herlawati , Atika Prima Dina , Yusuf Ajif Yunizar Pratama , Khasanah Fata Nidaul , Retnoningsih Endang , Sanusi Beno Aditya , Wakhid Gedhe Hilman
Published: March 2022

Getting information on routes can be he main problem for visitors. For example in determining the route to a proper place for eating and how to find the closest route to a mall. Based on the existing problems, this study proposes an application for finding information about places that visitors want to go based on the closest route. Algorithm A-Star (A*) was implemented that uses the distance estimation by finding the closest path to the destination using a heuristic function as a basis to select from several alternatives effectively. The result showed that an android application can give the information about the location of places to visit for eating and malls by calculating the distance from the starting point to the end point.
AKIBAT HUKUM PENERBITAN SURAT IZIN USAHA PERTAMBANGAN (IUP) PADA KEKAYAAN ALAM KEPULAUAN SANGIHE
Authors: Lutfulloh Zen , Donri Wahyu
Published: March 2022

Mining is a strategic sector that must be utilized by the government as well as possible. In accordance with the mandate contained in Article 33 of the 1945 Constitution, the government or the state is obliged to serve every citizen and resident to fulfill their basic needs in the context of public services and improving the welfare of the community. One of the public services that get a bad impression in the community is related to licensing services. At the beginning of 2021, there was a case phenomenon that became a polemic of debate among the community, government and environmental health observers. The phenomenon is that it is easy for the government to issue a mining business permit (IUP) decree to PT Tambang Mas Sangihe (TMS) which substantially violates Law Number 27 of 2007 concerning Management of Coastal Areas and Small Islands as well as having a large potential to cause environmental damage because some of the the area that gets the mining permit is a protected forest which is home to several endemic animals and plants that are threatened with extinction. This study uses normative research methods to determine the legal consequences of the policy. The purpose of this study is to provide several points of consideration to the government in terms of re-evaluating the decree. The results of this study indicate that Decree Number 163.K/MB.04/DJB/2021 has legal consequences for re-evaluation because in addition to violating other laws, the decision has the potential to cause damage to the natural environment in the Sangihe Islands.

Key Words: Mining, Licensing, Environment
KAJIAN KEPUASAN MASYARAKAT PENGHAYAT KEPERCAYAAN TERHADAP PELAYANAN DOKUMEN KEPENDUDUKAN DI KABUPATEN BREBES
Authors: Fikri Ahmad Fatkul , Tyas Titisari Haruming
Published: March 2022

The faith's adherents often get discriminated against in the service of population documents. Believers are required to fill in one of the official religions in Indonesia. The presence of the Decision of the Constitutional Court Number 97/PUU-XIV/2016 could not eliminate the act of discrimination. The state cannot guarantee the continuity of religion and belief that its adherents believe as guaranteed by the state constitution. This study aims to determine the Community Satisfaction Index (IKM) adherents of faith in the service of population documents in Brebes Regency with a quantitative descriptive approach. The sampling technique used is purposive sampling with data collection techniques of observation, documentation, and questionnaires. This research used Pearson's Product Moment validity test and Cronbach's alpha formula for reliability test to determine the questionnaire questions' validity. The study results show that the Community Satisfaction Index (IKM) of adherents' belief in services at the Population and Civil Registration Office of Brebes Regency is 2.83, with a conversion value of 70.75. So the performance of public services is of good quality. However, there are still service indicators in the poor category, namely equal rights and equal treatment/non-discrimination.
PENGEMBANGAN WISATA HALAL DI KOTA BANDA ACEH
Authors: Santoso Eko Budi , Rahmadanita Annisa , Rahmaniazar Luthfiani , Hidayat Enjang , Alyani Neni
Published: March 2022

Beberapa indikasi masalah di lapangan serta hasil penelitian terkait menunjukkan bahwa masih ada banyak masalah dalam pengembangan wisata halal di Provinsi NAD maupun di Kota Banda Aceh. Tujuan penelitian untuk mengetahui lebih dalam mengenai pengembangan wisata halal yang dilakukan di Kota Banda Aceh. Penelitian ini merupakan penelitian deskriptif kualitatif. Pengumpulan datanya dilakukan teknik wawancara, observasi, dan dokumentasi. Wawancaranya dilakukan terhadap 16 orang informan.  Untuk meningatkan validitas penelitian, penulis juga melakukan trianggulasi baik triangulasi sumber data maupun triangulasi teknik. Konsep “pengembangan” yang digunakan dalam penelitian ini, menggabungkan pendapat Sammeng (2001), Andrew E. Sikula dalam Sedarmayanti (2009) dan Yoeti (2008). Oleh karena itu konsep “pengembangan” dimaknai sebagai proses perubahan sistem yang dilakukan secara sadar dan terencana menuju kondisi yang lebih baik dalam aspek sumber daya manusia, aspek fisik maupun aspek non fisik lainnya. Sedangkan terkait penyelenggaraan wisata halal di Kota Aceh, digunakan dasar peraturan Wali Kota Banda Aceh Nomor 17 tahun 2016 tentang penyelenggaraan wisata halal. Hasil penelitian menemukan bahwa masih terdapat fasilitas yang kurang memadai, kurangnya jumlah pegawai, serta kurangnya pemahaman dan kesadaran masyarakat dalam pengembangan wisata halal di Kota Banda Aceh. Dari tiga dimensi yang diamati, yaitu dimensi pengembangan obyek dan destinasi wisata, dimensi penyediaan prasarana, serta dimensi pengembangan SDM wisata, kesemuanya menunjukkan kinerja yang belum dapat dinilai baik. Dapat disimpulkan bahwa pengembangan wisata halal di Kota Banda Aceh belum terlaksana dengan baik.

Kata Kunci: Pengembangan, Wisata Halal, Kota Banda Aceh.
PENGEMBANGAN KOMPETENSI APARATUR DI BADAN PENGELOLA PAJAK DAN RETRIBUSI DAERAH KOTA MEDAN PROVINSI SUMATERA UTARA
Authors: Hasibuan Carina Safitri , Saputra Romi , Nurrahman Agung
Published: March 2022

Sumber daya manusia aparatur menjadi salah satu dari delapan tujuan area perubahan reformasi birokrasi. Hal ini disebabkan karena sumber daya manusia aparatur merupakan pelaku yang bekerja dan menjalankan fungsi pemerintahan. Salah satu fungsi pemerintahan adalah menyelenggarakan pelayanan publik. Namun kenyataannya di lapangan masih banyak keluhan masyarakat terhadap pelayanan publik. Penelitian ini bertujuan untuk mengetahui dan menganalisis pengembangan kompetensi aparatur dalam meningkatkan pelayanan pajak bumi dan bangunan pada BPPRD di Kota Medan Provinsi Sumatera Utara. Penelitian ini merupakan jenis penelitian kualitatif deskriptif dengan pendekatan induktif. Pengumpulan datanya melalui wawancara, observasi, dan dokumentasi. Teknik analisis datanya menggunakan data reduction pemilihan data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menunjukkan kompetensi yang dimiliki aparatur BPPRD Kota Medan dalam pelayanan pajak dinilai kurang baik. Hal tersebut disebabkan oleh beberapa faktor yang dapat menghambat pengembangan kompetensi. Faktor penghambatnya yaitu keterbatasan anggaran dalam pengembangan kompetensi, keterbatasan jumlah kuota peserta pelatihan dan pendidikan, kurangnya penguasaan terhadap penggunaan teknologi informasi, serta sarana dan prasarana yang belum memadai.

Kata Kunci: Kompetensi, Pelayan Publik, Pajak dan Retribusi, Pelayanan Publik.
OMNIMBUS LAW DAN PENYUSUNAN RENCANA TATA RUANG: KONSEPSI, PELAKSANAAN DAN PERMASALAHANNYA DI INDONESIA
Authors: Pambudi Andi Setyo , Sitorus Santun R.P.
Published: March 2022

Fenomena permasalahan pembangunan terkait dengan perencanaan penataan ruang dan perencanaan pembangunan. Idealnya, penataan ruang dan pembangunan harus dilakukan secara terintegrasi, baik secara substansi, spasial maupun pendanaan. Secara konsep, penyusunan rencana tata ruang terkait dengan ekspresi spasial-geografis yang mencakup kebijakan perekonomian, sosial, lingkungan dan kebudayaan masyarakat. Perencanaan ruang berhubungan dengan pengembangan wilayah yang didalamnya terdapat sektor-sektor dengan sebaran sumber daya dan segala kegiatan dan permasalahannya dalam berbagai jenis dan skala. Makalah ini berusaha menjelaskan penyusunan rencana tata ruang, baik dari sisi konsepsi, pelaksanaan maupun permasalahan yang dihadapi, termasuk menyajikan permasalahan yang terjadi ditingkat tapak terkini. Metode yang digunakan adalah literature review berbasis informasi dari regulasi, jurnal, buku dan sumber lain yang relevan. Hasil analisis menunjukkan bahwa perencanaan tata ruang menghadapi tantangan adanya COVID-19 dan diterapkannya UU Cipta Kerja dan turunannya. Nuansa kental aspek “pemanfaatan” ruang dalam regulasi terkini terkait tata ruang yang dipengaruhi UU Cipta Kerja mengindikasikan bahwa pengendalian tata ruang menjadi tantangan tersendiri bagi para perencana.
KINERJA DINAS PEMBERDAYAAN MASYARAKAT DAN DESA DALAM MENINGKATKAN KUALITAS APARATUR DESA DI KABUPATEN LAMANDAU PROVINSI KALIMANTAN TENGAH
Authors: Rumbekwan Margaretha , Rahmadanita Annisa , Ajang Okky Irwanto , Santoso Eko Budi
Published: March 2022

Penelitian ini bertujuan untuk mendapatkan data dan informasi pelaksanaan Kinerja Dinas Pemberdayaan Masyarakat dan Desa dalam meningkatkan kualitas aparatur desa di Kabupaten Lamandau, dengan menggunakan teori kinerja dari T.R. Mitchell dalam Serdamayanti, (2009). Mengetahui faktor-faktor penghambat kinerja DPMD, dan upaya dalam mengatasi hambatan dalam meningkatkan kinerja DPMD. Penelitian ini merupakan penelitian kualitatif dengan metode deskriptif dengan pendekatan induktif, teknik pengumpulan data yang digunakan dalam penelitian ini menggunakan teknik observasi, wawancara dan dokumentasi adapun dalam menganalisis data yaitu pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa Kinerja Dinas Pemberdayaan Masyarakat dan Desa masih belum optimal dalam meningkatkan kualitas aparatur desa di Kabupaten Lamandau hal tersebut dilihat dari lima dimensi kinerja menurut T.R. Mitchell dimana tiga dimensi kinerjanya menunjukan performa masih belum optimal yaitu di dimensi Kualitas kerja, Kemampuan aparatur dan Disiplin kerja dan dua dimensi sudah cukup baik yaitu di dimensi Inisiatif dan Komunikasi.

 

Kata Kunci: Kinerja, Peningkatan Kualitas Aparatur, Pemerintah Desa
The apparent resurrection of Jesus is verisimilar
Authors: Keenan Douglas
Published: March 2022

The vital medical aspects of crucifixion are known to be similar to some medical aspects of drowning. And some people have been able to survive being underwater, without breathing, for tens of minutes (e.g. Bolte et al., Journal of the American Medical Association, 1988). We argue that some of the physiological mechanisms that enabled those people to survive could also account for Jesus being able to survive his crucifixion. Those mechanisms are known to be activated by certain conditions—and the conditions are demonstrated to be described in the biblical story. Thus, the apparent resurrection of Jesus has a reasonable naturalistic explanation.
Intervenciones asistidas con animales en las ciencias del deporte: una propuesta de inclusión entre las actividades físicas en el medio natural.
Authors: Díaz Hernández Myriam , Lavín Pérez Ana Myriam , Villafaina Santos , Domínguez-Muñoz Francisco Javier , Morenas Jesús , Adsuar José Carmelo , González-García Israel , Collado Mateo Daniel
Published: March 2022

Las intervenciones asistidas con animales (IAA), en sus diferentes formas, han mostrado beneficios en las personas a diferentes niveles. En España, profesionales de diferentes ámbitos llevan a cabo este tipo de intervenciones, destacando la ausencia de profesionales de las ciencias del deporte (CAFYD). Este artículo tiene como objetivo valorar la pertinencia del profesional de CAFYD en las IAA, y más concretamente, dentro de las actividades físicas en la naturaleza. La metodología se ha centrado en la realización de una revisión sistemática de la literatura científica sobre sus beneficios en la condición física. Posteriormente se realizado un análisis crítico sobre la relación entre las IAA y las CAFYD, para posteriormente desarrollar la posible inclusión de las IAA como actividades físicas en el medio natural, de acuerdo con las diferentes clasificaciones existentes y atendiendo a las características propias de este tipo de actividades. Los resultados de la revisión de revisiones mostraron que las IAA tienen numerosos beneficios a nivel físico (equilibrio, movilidad o marcha), lo cual estaría relacionado con el componente motriz de las IAA y justificaría la presencia de profesionales en CAFYD. Además, la interacción humano-animal podría relacionarse también con los beneficios descritos mediante el concepto de biofilia, presente en las actividades físicas en la naturaleza
Efecto de un entrenamiento vibratorio sobre el dolor de espalda en mujeres sedentarias con fibromialgia
Authors: Morales-Castaño Ricardo , Denche-Zamorano Ángel Manuel , Mendoza-Muñoz María , Carlos-Vivas Jorge , Rojo-Ramos Jorge , Barrios Fernandez Sabina
Published: March 2022

El objetivo de este estudio fue estudiar el efecto de un entrenamiento de Vibracio?n Meca?nica de Cuerpo Completo (VMCC) de 12 semanas en el dolor de espalda de personas sedentarias con Fibromialgia (FM). 41 mujeres diagnosticadas con FM de una media de 47 an?os (±10.5) fueron aleatorizadas en un grupo control (n= 20) y grupo de VMCC (n=21). La intervencio?n de VMCC consistio? en 6 repeticiones a 12,5 Hz repartidas en 3 sesiones por semana durante 12 semanas. Se midio? el dolor de espalda con el cuestionario Roland Morris y el grado de discapacidad con el Cuestionario de Impacto de FM. Tras la realizacio?n del programa VMCC no se han obtenido diferencias estadi?sticamente significativas en el dolor de espalda. Como conclusio?n, el programa propuesto no ha mejorado el dolor especi?fico de espalda en mujeres sedentarias con FM
Efectos de las intervenciones digitales para mejorar la salud en edad escolar: una revisión sistemática.
Authors: Romero-Roso Lucía , Rojo-Ramos Jorge , Carlos-Vivas Jorge , Barrios Fernandez Sabina , Pastor-Cisneros Raquel , Denche-Zamorano Ángel Manuel
Published: March 2022

El objetivo de esta revisión sistemática fue examinar las intervenciones digitales y/o acelerometría para aumentar la actividad física y prevenir conductas sedentarias, dieta no saludable y/o problemas de sueño en niños y adolescentes dentro del ámbito educativo. Método: se han identificado los estudios en dos bases de datos (PubMed y Scopus). El proceso de búsqueda ha sido desde enero de 2015 a mayo de 2020 con el fin de encontrar todos los estudios similares publicados sobre el tema. Un total de 12 artículos han cumplido con los criterios de inclusión. Resultados: 8 estudios han mostrado efectos significativos en los hábitos saludables definidos. Conclusiones: las intervenciones que utilizaron la tecnología digital y/o acelerometría fueron beneficiosas para reducir conductas sedentarias, aumentar la actividad física y la dieta saludable en niños y adolescentes. Se recomiendan más estudios sobre estrategias digitales para conseguir la adherencia a los hábitos saludables a través de programas educativos de salud escolar, coordinación efectiva y un mayor seguimiento a largo plazo en esta población.
Akran Öğretiminin Öğrencilerin Çözünme Olgusu ve Çözeltilerin Fiziksel Özellikleri ile İlgili Anlayışlarına Etkisi
Authors: YILDIRIM Tamer , CANPOLAT Nurtaç
Published: March 2022

Bu çalışmada; akran öğretimi yönteminin öğrencilerin çözünme olgusu ve çözeltilerin fiziksel özellikleri ile ilgili kavramsal anlayışları üzerine etkisi incelenmiştir. Durum incelemesi (case study) yöntemi ile yürütülen araştırmada veri toplama aracı olarak öğrencilerden mülakat ile görüşleri yazılı olarak alınmıştır. Araştırmanın çalışma grubu, 2016-2017 eğitim yılında Artvin İskebe Anadolu Lisesinin 11. sınıfta öğrenime devam eden 59 öğrenciden oluşmaktadır. Görüşme esnasında yazılı olarak öğrencilerden şekil ve grafik çizmeleri istenmiştir. Elde edilen veriler betimsel olarak analiz edilmiştir. Bulgular betimlenerek tablolar halinde sunulmuştur. Araştırma sonucuna göre akran öğretimi yönteminin öğrencilerde daha derinlemesine öğrenmeyi sağladığı tespit edilmiştir.
A Morphological Study of the Meniscus, Cartilage and Subchondral Bone Following Closed-Joint Traumatic Impact to the Knee
Authors: Haut Donahue T. L. , Narez G. E. , Powers M. , Dejardin L. M. , Wei F. , Haut R. C.
Published: March 2022

Post-traumatic osteoarthritis (PTOA) is a debilitating disease that is a result of a breakdown of knee joint tissues following traumatic impact. The interplay of how these tissues influence each other has received little attention because of complex interactions. This study was designed to correlate the degeneration of the menisci, cartilage and subchondral bone following an acute traumatic event that resulted in anterior cruciate ligament (ACL) and medial meniscus tears. We used a well-defined impact injury animal model that ruptures the ACL and tears the menisci. Subsequently, the knee joints underwent ACL reconstruction and morphological analyses were performed on the menisci, cartilage and subchondral bone at 1-, 3- and 6-months following injury. The results showed that the morphological scores of the medial and lateral menisci worsened with time, as did the tibial plateau and femoral condyle articular cartilage scores. The medial meniscus was significantly correlated to the medial tibial subchondral bone at 1 month (p = 0.01), and to the medial tibial cartilage at 3 months (p = 0.04). There was only one significant correlation in the lateral hemijoint, i.e., the lateral tibial cartilage to the lateral tibial subchondral bone at 6 months (p = 0.05). These data may suggest that, following trauma, the observed medial meniscal damage should be treated acutely by means other than a full or partial meniscectomy, since that procedure may have been the primary cause of degenerative changes in the underlying cartilage and subchondral bone. In addition to potentially treating meniscal damage differently, improvements could be made in optimizing treatment of acute knee trauma.
Coagulation Factor IIIa (f3a) Knockdown in Zebrafish Leads to Defective Angiogenesis and Mild Bleeding Phenotype
Authors: Subramaniam Saravanan , Liu Jiandong , Fletcher Craig , Ramchandran Ramani , Weiler Hartmut
Published: March 2022

Tissue factor (TF) is crucial for embryogenesis, as mice lacking TF are embryonically lethal (E10.5). This lethality may be attributed to defects in vascular development and circulatory failure, suggesting additional roles for TF in embryonic development beyond coagulation. In this study, we characterized the role of one of the TF paralogs (f3a) using a zebrafish model. The expression of f3a during embryonic developmental stages was determined by RT-PCR. Spatiotemporal expression pattern of f3a revealed (high expression from 28 to 36 hpf) the role of in the development of the yolk sac, circulation, and fins. Morpholinos (MO), an antisense-based oligonucleotide strategy, was used to knockdown f3a and examined for defects in morphological appearance, bleeding, and vascular patterning. f3a MO-injected embryos showed morphological abnormalities, including shorter body lengths and crooked tails. O-dianisidine staining showed f3a MO-injected embryos exhibited bleeding in the trunk (5.44%) and head (9.52%) regions. Imaging of endothelial-specific transgenic lines (flk1:egfp-NLS/kdrl:mCherry-CAAX) showed a 3-fold decreased caudal vein plexus (CVP) in f3a morphants versus controls at 48 hpf, suggesting a potential role for f3a in angiogenesis. These findings confirm that f3a is essential for angiogenesis, in addition to its involvement in hemostasis.
The Pro-fibrotic Response of Mesenchymal Leader Cells to Lens Wounding Involves Hyaluronic Acid, Its Receptor RHAMM, and Vimentin
Authors: Menko A. Sue , Romisher Alison , Walker Janice L.
Published: March 2022

Hyaluronic Acid/Hyaluronan (HA) is a major component of the provisional matrix deposited by cells post-wounding with roles both in regulating cell migration to repair a wound and in promoting a fibrotic outcome to wounding. Both are mediated through its receptors CD44 and RHAMM. We now showed that HA is present in the provisional matrix assembled on the substrate surface in a lens post-cataract surgery explant wound model in which mesenchymal leader cells populate the wound edges to direct migration of the lens epithelium across the adjacent culture substrate onto which this matrix is assembled. Inhibiting HA expression with 4-MU blocked assembly of FN-EDA and collagen I by the wound-responsive mesenchymal leader cells and their migration. These cells express both the HA receptors CD44 and RHAMM. CD44 co-localized with HA at their cell-cell interfaces. RHAMM was predominant in the lamellipodial protrusions extended by the mesenchymal cells at the leading edge, and along HA fibrils organized on the substrate surface. Within a few days post-lens wounding the leader cells are induced to transition to αSMA+ myofibroblasts. Since HA/RHAMM is implicated in both cell migration and inducing fibrosis we examined the impact of blocking HA synthesis on myofibroblast emergence and discovered that it was dependent on HA. While RHAMM has not been previously linked to the intermediate filament protein vimentin, our studies with these explant cultures have shown that vimentin in the cells’ lamellipodial protrusions regulate their transition to myofibroblast. PLA studies now revealed that RHAMM was complexed with both HA and vimentin in the lamellipodial protrusions of leader cells, implicating this HA/RHAMM/vimentin complex in the regulation of leader cell function post-wounding, both in promoting cell migration and in the transition of these cells to myofibroblasts. These results increase our understanding of how the post-wounding matrix environment interacts with receptor/cytoskeletal complexes to determine whether injury outcomes are regenerative or fibrotic.
Exfoliation of Al-Residual Multilayer MXene Using Tetramethylammonium Bases for Conductive Film Applications
Authors: Saita Emi , Iwata Masaki , Shibata Yuki , Matsunaga Yuki , Suizu Rie , Awaga Kunio , Hirotani Jun , Omachi Haruka
Published: March 2022

This study describes the concise exfoliation of multilayer Ti3C2Tx MXene containing residual aluminum atoms. Treatment with tetramethylammonium base in a co-solvent of tetrahydrofuran and H2O produced single-layer Ti3C2Tx, which was confirmed via atomic force microscopy observations, with an electrical conductivity 100+ times that of Ti3C2Tx prepared under previously reported conditions. The scanning electron microscopy and X-ray diffraction measurements showed that the exfoliated single-layer Ti3C2Tx MXenes were reconstructed to assembled large-domain layered films, enabling excellent macroscale electric conductivity. X-ray photoelectron spectroscopy confirmed the complete removal of residual Al atoms and the replacement of surface fluorine atoms with hydroxy groups. Using the exfoliated dispersion, a flexible transparent conductive film was formed and demonstrated in an electrical application.
High Density Lipoprotein Reduces Blood Pressure and Protects Spontaneously Hypertensive Rats Against Myocardial Ischemia-Reperfusion Injury in an SR-BI Dependent Manner
Authors: Al-Jarallah Aishah , Babiker Fawzi
Published: March 2022

Background

Hypertension is a key risk factor in the development of cardiovascular diseases. Elevation in blood pressure alters high density lipoprotein (HDL) function and composition. The exact role of HDL in cardiovascular complications observed in hypertension is however not clearly understood. HDL protected against myocardial ischemia/reperfusion (I/R) injury in normotensive rats. Nonetheless, it's not clear if restoration of HDL function and/or composition protects against myocardial I/R injury in spontaneously hypertensive rats (SHR).

Objectives

In this study we tested the effect of HDL treatment on I/R injury in Wistar Kyoto rats (WKY) and SHR and investigated the possible underlying mechanism(s).

Methods

HDL (900 ng/kg/min) or vehicle were continuously administered to 11-week old WKY and SHR for 1 week (chronic treatment). Blood pressure was measured before and after treatment. Hearts were subjected to I/R injury using a modified Langendorff system. Another set of rats were treated with HDL administered at reperfusion (acute treatment) in the presence or absence of scavenger receptor class B type-I (SR-BI) blocking antibody. Cardiac hemodynamics were computed and cardiac enzyme release and infarct size were measured. Total cholesterol (TC) and HDL-cholesterol (HDL-C) were enzymatically assayed. Markers of autophagy and inflammation were detected by immunoblotting and ELISA, respectively.

Results

HDL treatment did not increase TC or HDL-C levels in SHR or WKY, yet it significantly (P < 0.01) reduced systolic and diastolic blood pressure in SHR. Chronic and acute HDL treatment significantly (P < 0.05) protected WKY and SHR against myocardial I/R injury. Chronic HDL treatment was significantly (P < 0.05) more protective in SHR whereas acute HDL treatment induced significantly (P < 0.05) greater protection in WKY. The extent of HDL induced protection was proportional to the expression levels of cardiac SR-BI and blockage of SR-BI completely abolished HDL mediated protection in SHR. Chronic HDL treatment significantly (P < 0.05) reduced markers of autophagy and inflammation in hypertensive rats.

Conclusions

We demonstrate a novel anti-hypertensive and a cardioprotective effect of HDL against myocardial I/R injury in SHR, the magnitude of which is directly related to the expression levels of cardiac SR-BI. Mechanistically, chronic HDL treatment protected SHR hearts by reducing autophagy and inflammation.
Identification and Analysis of Hub Genes in Diabetic Cardiomyopathy: Potential Role of Cytochrome P450 1A1 in Mitochondrial Metabolism and STZ-Induced Myocardial Dysfunction
Authors: Chen Yinliang , Yang Jinbao , Wang Ying , Shen Weike , Liu Jinlin , Yuan Meng , Hao Xiaoyu , Zhong Li , Guo Rui
Published: March 2022

Diabetic cardiomyopathy (DCM) is a primary cause of death in diabetic patients; however, its molecular mechanism is not yet clear, and there is no uniform standard for diagnosis. The aim of this study is to discover the pathogenesis and potential therapeutic targets of DCM through screening and analysis of differentially expressed genes (DEGs) in heart ventricles of DCM, and to testify the role of key hub genes in DCM-induced myocardial dysfunction. Datasets GSE4745 and GSE6880 were downloaded from the GEO database. The difference analysis, visual analysis, cluster analysis and enrichment analysis were performed by using R language, python scripts and bioinformatics software followed by the construction of protein-protein interaction (PPI) network to obtain hub genes. The DCM models were established by streptozocin (STZ) injection to the male mice. The cardiac function and the expressions of hub genes were examined by using echocardiography and real-time quantitative poly-merase chain reaction (RT-qPCR), followed by multiple statistical analyses. Bioinformatic results indicate that mitochondrial dysfunction, disturbed lipid metabolism and decreased collagen synthesis are the main causes of the DCM development. In particular, the hub gene Cyp1a1 that encodes Cytochrome P450 1A1 (CYP4501A1) enzyme has the highest connectivity in the interaction network, and is associated with mitochondrial homeostasis and energy metabolism. It plays a critical role in the oxidation of endogenous or exogenous substrates. Our RT-qPCR results confirmed that ventricular Cyp1a1 mRNA level was nearly 12-fold upregulated in DCM model compared to normal control, which was correlated with abnormal cardiac function in diabetic individuals. CYP4501A1 protein expression in mitochondria was also increased in diabetic hearts. However, we found no significant changes in collagen expressions in cardiac ventricles of mice with DCM. This study provided compact data support for understanding the pathogenesis of DCM. CYP4501A1 might be considered as a potential candidate targeting for DCM therapy. Follow-up animal and clinical verifications need to be further explored.
Study on Mechanical Behavior of Slip Zone Soils Under Different Factors—A Case Study
Authors: Liu Kai , Wang Xingang , Lian Baoqin , Zhu Zhaobo , Xue Chen
Published: March 2022

The mechanical properties of the slip zone soil play an important role in the evolution of the loess landslides. To further understand these characteristics, a series of ring shear tests was conducted on the slip zone soils obtained from Tianshuigou landslide, to investigate the influence of moisture content, dry density, shear rate and shear method on the mechanical characteristics of slip zone soils. The experimental results showed that: an increase in the moisture content of the slip zone loess causes a significant reduction in the residual strength. Specially, both the residual cohesion and residual internal friction angle show a deceasing tendency with moisture content. The change in the residual cohesion is more sensitive to the variation in moisture content. Additionally, a trend that strength increased with the increasing of dry density was observed, and the influence degree of dry density on the increased strength is more pronounced at low moisture contents. Thirdly, shear strength shows a negative relationship with shear rate when the shear rate ranges from 0.01 mm/min and 1 mm/min. When the shear rate increased up to 10 mm/min, a stepped shear band is developed and the strength increased. In addition, the strain-softening phenomenon was observed in the single-stage shear tests, which was not noticed in the multi-stage shear tests and pre-shear tests. The residual strength obtained in pre-shear test and multi-stage shear test is slightly greater than that in the single-stage shear test. The experimental results herein can provide an important basis for analyzing the evolution mechanism and prevention of loess landslides.
Geochemical Characteristics of Rare Earth Elements in the Chaluo Hot Springs in Western Sichuan Province, China
Authors: Wei Shuaichao , Liu Feng , Zhang Wei , Zhang Hanxiong , Yuan Rouxi , Liao Yuzhong , Yan Xiaoxue
Published: March 2022

High-temperature hydrothermal activity areas in western Sichuan Province, China are ideal objects for studying deep Earth science, extreme ecological environments, and comprehensive geothermal utilization. To understand the geochemical characteristics of rare Earth elements (REEs) in the Chaluo hot springs in western Sichuan Province, the authors analyzed the composition and fractionation of REEs in the hot springs through hydrochemical analysis, REE tests, and North American Shale Composite-normalized REE patterns. Moreover, the composition and complex species of REEs in the geothermal water in the Chaluo area were determined through calculation and simulation analysis using the Visual MINTEQ 3.0 software. The results are as follows. In terms of hydrochemical type, all geothermal water in the Chaluo area is of the Na-HCO3 type. The cations in the geothermal water are mainly controlled by water-rock interactions and evaporation, the anions are determined by water-rock interactions, and the hydrochemical processes are primarily controlled by the dissolution of silicate minerals. The total REE content of the geothermal water in the Chaluo hot springs is 0.306 ± 0.103 ug/L. It is low compared to the Kangding area and is primarily affected by the reductive dissolution of Fe oxides/hydroxides, followed by pH. The geothermal water in the Chaluo area is rich in light rare Earth elements (LREEs) because of the presence of Fe oxides. It shows positive Eu and Ce anomalies due to the combined effects of the dissolution of Eh and Mn oxides and surface water. Furthermore, the positive Eu anomalies are also caused by the water-rock interactions between the Qugasi Formation and deep geothermal water. Similar to alkaline water bodies, the complex species of REEs in the geothermal water mainly include Ln(CO3)2−, LnCO3+, and LnOH2+, which is caused by the stability constants of complexation reactions.
Managing Stakeholder Concerns in Green Building Projects With a View Towards Achieving Social Sustainability: A Bayesian-Network Model
Authors: Wen Shiping , Qiang Guofeng
Published: March 2022

Green building projects (GBPs) involve multiple interdependent stakeholders, whose individual and separate concerns have different degrees of impact on sustainability management. These concerns are highly complex, subject to many uncertainties, and pose significant challenges to decision-makers during sustainability assessments, especially with regard to the social aspects of the project. As such, addressing the complexity of stakeholder concerns and optimizing the decision-making process in green building projects from the stakeholder perspective are crucial to improving practices in social sustainability management. However, to date, there is a lack of relevant empirical studies on this subject. This study proposes a decision-making model based on Bayesian networks (BN); a project network decision model is also constructed from a social sustainability perspective. A diagnostic analysis and sensitivity analysis of the constructed model identify the key stakeholder concerns that affect the social sustainability of the project. To verify its feasibility, the BN model is applied to a green building project, specifically, the Wuhan International Commerce Center, China. The results identify green design and construction, an abundance and stability of project funds, and conveniently-situated service facilities as the primary, sensitive stakeholder concerns that significantly impact social sustainability. The findings show that the BN model can be used as a long-term management decision-making tool for this project. The uncertainty problem associated with changes in sustainability levels induced by the multiplicity of stakeholders is addressed in this study. Furthermore, the findings expand the topic of social sustainability in green construction projects. These findings aid project decision-makers in managing stakeholders individually based on their various concerns, as well as improving the social sustainability of green building projects.
Use of miRNA Sequencing to Reveal Hub miRNAs and the Effect of miR-582-3p/SMAD2 in the Progression of Hepatocellular Carcinoma
Authors: Zhao Yi , Li Meizhang , Miao Nana , Wei Wei , Dong Yulong , Tao Chenjie , Chen Jinzhong , Pei Yongyan , Guo Lieping
Published: March 2022

Hepatocellular carcinoma is a common tumor with a high fatality rate worldwide, and exploring its pathogenesis and deterioration mechanism is a focus for many researchers. Increasing evidence has shown that miRNAs are involved in the occurrence and progression of a variety of cancers, including hepatocellular carcinoma. Therefore, this study mainly aimed identify key miRNAs related to hepatocellular carcinoma and explore their potential functions and clinical significance. In this study, we performed miRNA sequencing on three pairs of hepatocellular carcinoma tissue samples and screened 26 differentially expressed miRNAs. Then 2 key miRNAs (miR-139-5p and miR-582-3p) were screened by Kaplan-Meier curve analysis, Cox multivariate analysis and qPCR methods. The expression of miR-582-3p was positively correlated with clinicopathological parameters in patients with hepatocellular carcinoma. Subsequently, miRwalk and starbase were used to predict the target genes of key miRNAs, and then the key pairs miR-582-3p/SMAD2 identified by WGCNA, PPI, qPCR and Pearson correlation analysis. Finally, a dual luciferase experiment, the rescue-of-function experiment and qPCR confirmed that miR-582-3p directly targets SMAD2 and regulates the proliferation, migration and invasion of HepG2 cells by targeting SMAD2. At the same time, interference with SMAD2 can influence the effect of miR-582-3p on HepG2 cells. In conclusion, our findings confirm that miR-582-3p is an independent factor for the prognosis of hepatocellular carcinoma patients, and can regulate the progression of hepatocellular carcinoma cells by targeting SMAD2.
Fast All-Electron Hybrid Functionals and Their Application to Rare-Earth Iron Garnets
Authors: Redies Matthias , Michalicek Gregor , Bouaziz Juba , Terboven Christian , Müller Matthias S. , Blügel Stefan , Wortmann Daniel
Published: March 2022

Virtual materials design requires not only the simulation of a huge number of systems, but also of systems with ever larger sizes and through increasingly accurate models of the electronic structure. These can be provided by density functional theory (DFT) using not only simple local approximations to the unknown exchange and correlation functional, but also more complex approaches such as hybrid functionals, which include some part of Hartree–Fock exact exchange. While hybrid functionals allow many properties such as lattice constants, bond lengths, magnetic moments and band gaps, to be calculated with improved accuracy, they require the calculation of a nonlocal potential, resulting in high computational costs, that scale rapidly with the system size. This limits their wide application. Here, we present a new highly-scalable implementation of the nonlocal Hartree-Fock-type potential into FLEUR—an all-electron electronic structure code that implements the full-potential linearized augmented plane-wave (FLAPW) method. This implementation enables the use of hybrid functionals for systems with several hundred atoms. By porting this algorithm to GPU accelerators, we can leverage future exascale supercomputers which we demonstrate by reporting scaling results for up to 64 GPUs and up to 12,000 CPU cores for a single k-point. As proof of principle, we apply the algorithm to large and complex iron garnet materials (YIG, GdIG, TmIG) that are used in several spintronic applications.
Interstitial Lung Disease in Firefighters: An Emerging Occupational Hazard
Authors: Lee Cathryn T. , Ventura Iazsmin Bauer , Phillips E. Kate , Leahy Amy , Jablonski Renea , Montner Steven , Chung Jonathan H. , Vij Rekha , Adegunsoye Ayodeji , Strek Mary E.
Published: March 2022

Introduction

Occupational risk factors for interstitial lung disease (ILD) are a remediable aspect of this progressive pulmonary disorder. The association between firefighting and ILD is unknown. Our objective was to assess the characteristics of firefighters with ILD from a large single-center ILD registry.

Methods

The University of Chicago ILD database was reviewed for patients with a history of firefighting. Clinical information was abstracted from the medical record. The prevalence rate ratio of firefighters in the database compared to the baseline prevalence of firefighting in the Chicago metropolitan area was calculated via the Poisson distribution.

Results

Nineteen firefighters were identified; all were men. A variety of ILD subtypes were seen across the cohort, including four patients with a diagnosis of connective tissue disease. Patients had mild forced vital capacity (FVC) and moderate diffusing capacity for carbon monoxide (DLCO) decrements on presentation; three patients died and two received lung transplantation over an average follow-up time of 76 months. Firefighters were seen at a greater proportion in the ILD registry than in the general population with a prevalence rate ratio of 3.98.

Conclusions

Firefighting was overrepresented in our cohort compared to the general population, suggesting that there may be a causative association between firefighting and the presence of ILD. The wide variety of ILD subtypes observed suggest that all ILD patients should be asked about their occupational history. Further investigation to identify occupational exposures and determine the benefit of remediation is needed.
Human Immunodeficiency Virus Type 1 Vif Up-Regulates the Expression of Tat via AKT Signaling Pathway: Role of Ubiquitin Specific Protease 17
Authors: Lata Sneh , Sood Vikas , Banerjea Akhil C.
Published: March 2022

Human immunodeficiency virus type 1 (HIV-1) has RNA genome and depends on host cellular machinery for most of its activities. Host cellular proteins modulate the expression and activity of viral proteins to combat the virus. HIV-1 proteins are known to regulate each other for the benefit of virus by exploiting these modulations. Here, we report that HIV-1 Vif increases the levels of Tat via AKT signaling pathway. We show that HIV-1 Vif activates AKT signaling pathway by inducing phosphorylation of AKT. Mdm2, downstream target of AKT signaling, increases the levels of Tat protein in ubiquitin-dependent manner by inducing Ubiquitin Specific Protease 17 (USP17), which is a deubiquitinase and stabilizes Tat protein. Thus, HIV-1 proteins exploit AKT signaling pathway to promote viral replication.