bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Factors Influence the Prognosis of Diabetic Macular Edema after Pars Plana Vitrectomy with Internal Limiting Membrane Peeling
Authors: Yamada Yoshihisa , Suzuma Kiyoshi , Kumagami Takeshi , Fujikawa Azusa , Kitaoka Takashi
Published: December 2012

Background: To evaluate the prognostic factors for the best-corrected visual acuity (BCVA) and foveal average retinal thickness after vitrectomy with internal limiting membrane (ILM) peeling for diabetic macular edema. Design: Retrospective, single-center study. Participants: This study involved 31 eyes of 27 patients who had undergone vitrectomy with ILM peeling between January 2005 and March 2008. Methods: Relationships between preoperative systemic or ocular factors and BCVA or foveal average retinal thickness before and 6 months after the operation were evaluated. Main Outcome Measures: BCVA and foveal average retinal thickness before and 6 months after the operation. Results: The mean logarithm of the minimum angle of resolution improved from 0.84 ± 0.64 (mean ± standard deviation) preoperatively to 0.64 ± 0.38 six months postoperatively (p = 0.393). Foveal average retinal thickness significantly improved from 473 ± 146 µm preoperatively to 318 ± 108 µm 6 months after the operation (p < 0.0001). Preoperative foveal average retinal thickness was significantly thicker with cardiovascular disease or cerebral infarction (p = 0.0019) or cystoid macular edema (p = 0.0028), while preoperative BCVA was significantly lower when an epiretinal membrane (p = 0.042) was present. Foveal average retinal thickness at the 6-month follow-up was significantly thicker when patients had a higher body mass index (p = 0.0088), were not on dialysis (p = 0.012), or did not have proliferative diabetic retinopathy (p = 0.013). BCVA at the 6-month follow-up was significantly lower in the group with no history of diabetes treatment until diabetic retinopathy was found (p = 0.023) and in patients with a higher preoperative glycosylated hemoglobin (p = 0.033). Conclusions: Preoperatively, BCVA and foveal average retinal thickness were primarily associated with ocular factors, while they were strongly associated with systemic factors, postoperatively. Ocular factor improvements may be related to the surgical procedure.
Intravitreal Ranibizumab for Acute Central Serous Chorioretinopathy
Authors: Kim Moosang , Lee Seung-Chan , Lee Seung-Jun
Published: December 2012

Background/Aims: To evaluate the effectiveness of intravitreal ranibizumab injection (IVRI) for acute central serous chorioretinopathy (CSC). Methods: Patients with symptomatic CSC of less than 3 months were prospectively recruited. Patients (n = 20/group) were randomly assigned to IVRI (0.5 mg/0.05 ml) or observation and followed for 6 months. logMAR best-corrected visual acuity (BCVA), fluorescein angiography, indocyanine angiography, and central foveal thickness (CFT) were assessed at baseline and at regular follow-ups. Results: All patients had increased BCVA, decreased CFT, and resolution of the neurosensory detachment. Complete resolution of neurosensory retinal detachment required more time in the observation group (13.0 ± 3.1 vs. 4.2 ± 0.9 weeks; p < 0.001). Mean BCVA and mean CFT improved significantly in both groups, but the changes were not significantly different between groups at 6 months. Conclusions: IVRI for acute CSC might hasten resolution of neurosensory detachment compared to observation alone. At 6 months, BCVA and CFT did not differ between IVRI and observation groups. Further studies are required to determine the long-term benefits of IVRI.
Changes in the Cell Surface Hydrophobicity of Oral Candida albicans from Smokers, Diabetics, Asthmatics, and Healthy Individuals following Limited Exposure to Chlorhexidine Gluconate
Authors: Ellepola Arjuna N.B. , Joseph Bobby K. , Khan Z.U.
Published: December 2012

Objective: The objective of this study was to determine the cell surface hydrophobicity of 40 oral Candida albicans isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, and healthy individuals, following brief exposure to subtherapeutic concentrations of chlorhexidine gluconate. Materials and Methods: Forty C. albicans oral isolates (10 isolates each from smokers, diabetics, asthmatics using steroid inhalers, and healthy individuals) were exposed to 3 subtherapeutic concentrations of chlorhexidine gluconate (0.00125, 0.0025, and 0.005%) for 30 min. Thereafter, the antiseptic was removed and the cell surface hydrophobicity was measured by a biphasic aqueous-hydrocarbon assay. Results: Compared to the unexposed controls, the cell surface hydrophobicity of C. albicans isolates was suppressed by 5.40% (p > 0.05), 21.17% (p < 0.05), and 44.67% (p < 0.05) following exposure to 0.00125, 0.0025, and 0.005% chlorhexidine gluconate, respectively. Conclusions: A brief period of transient exposure to subtherapeutic concentrations of chlorhexidine gluconate may modulate the cell surface hydrophobicity of C. albicans isolates and thereby may reduce candidal pathogenicity.
Genetic Loci for Coronary Calcification and Serum Lipids Relate to Aortic and Carotid Calcification
Authors: Bos Daniel , Ikram M. Arfan , Isaacs Aaron , Verhaaren Benjamin F.J. , Hofman Albert , van Duijn Cornelia M. , Witteman Jacqueline C.M. , van der Lugt Aad , Vernooij Meike W.
Published: December 2012

Background—

Atherosclerosis in different vessel beds shares lifestyle and environmental risk factors. It is unclear whether this holds for genetic risk factors. Hence, for the current study genetic loci for coronary artery calcification and serum lipid levels, one of the strongest risk factors for atherosclerosis, were used to assess their relation with atherosclerosis in different vessel beds.

Methods and Results—

From 1987 persons of the population-based Rotterdam Study, 3 single-nucleotide polymorphisms (SNPs) for coronary artery calcification and 132 SNPs for total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol or triglycerides were used. To quantify atherosclerotic calcification as a marker of atherosclerosis, all participants underwent nonenhanced computed tomography of the aortic arch and carotid arteries. Associations between genetic risk scores of the joint effect of the SNPs and of all calcification were investigated. The joint effect of coronary artery calcification–SNPs was associated with larger calcification volumes in all vessel beds (difference in calcification volume per SD increase in genetic risk score: 0.15 [95% confidence interval, 0.11–0.20] in aorta, 0.14 [95% confidence interval, 0.10–0.18] in extracranial carotids, and 0.11 [95% confidence interval, 0.07–0.16] in intracranial carotids). The joint effect of total cholesterol SNPs, low-density lipoprotein SNPs, and of all lipid SNPs together was associated with larger calcification volumes in both the aortic arch and the carotid arteries but attenuated after adjusting for the lipid fraction and lipid-lowering medication.

Conclusions—

The genetic basis for aortic arch and carotid artery calcification overlaps with the most important loci of coronary artery calcification. Furthermore, serum lipids share a genetic predisposition with both calcification in the aortic arch and the carotid arteries, providing novel insights into the cause of atherosclerosis.
South-South Trade and the Environment: A Brazilian Case Study
Authors: Hochstetler Kathryn
Published: December 2012

As South-South trade gains new weight in global exchange patterns, will environmental protection be enhanced or endangered? Environmental economists are generally optimistic that trade will lead to greater environmental protection, but see less chance of that in South-South trade; political economists make opposite arguments on both points. This article shows that South-South trade is dominated by a small set of fourteen countries, with most Southern countries continuing to be natural resource providers. Case studies of Brazil's trade with China and with its South American neighbors reveal a policy framework that supports both of the opposed arguments: Southern countries can and do consider the environmental impacts of their production and trade, but strong counter-forces limit that effect.
Is There a Democracy–Civil Society Paradox in Global Environmental Governance?
Authors: Bernauer Thomas , Böhmelt Tobias , Koubi Vally
Published: December 2012

Civil society is commonly assumed to have a positive effect on international cooperation. This paper sheds light on one important facet of this assumption: we examine the impact of environmental non-governmental organizations (ENGOs) on ratification behavior of countries vis-à-vis international environmental agreements (IEAs). The main argument of the paper focuses on a “democracy-civil society paradox”: although ENGOs have a positive effect on ratification of IEAs on average, this effect decreases with increasing levels of democracy. This argument is counter-intuitive and appears paradoxical because democracy is generally associated both with a more active civil society and more international cooperation. The reasons for this hypothesized effect pertain to public demand for environmental public goods provision, government incentives, and problems of collective action among ENGOs. To test the net effect of ENGOs on countries' ratification behavior, the paper uses a new dataset on ENGOs in the time-period 1973–2006. The results offer strong support for the presumed democracy–civil society paradox.
Global Cities and Transnational Climate Change Networks
Authors: Lee Taedong
Published: December 2012

Why do some cities join transnational climate change networks while others do not? This study examines the factors that drive cities' participation in transnational climate change networks, such as the C40 Cities Climate Leadership Group and the Cities for Climate Protection program. Hierarchical analysis of 256 cities in 118 countries suggests that the degree of cities' globalization, or their level of “global cityness,” is positively associated with the cities' membership in the global networks. The level of individual cities' integration into the international economy and transportation grid is crucial for sharing ideas of global environmental responsibility. This tendency is found both in global cities of both developing and developed countries. Hierarchical models also suggest that attributes of cities—not country attributes such as democracy, income level, and being an Annex I country under the Kyoto Protocol—account for cities' memberships in transnational networks.
Protecting the Self: The Effect of Social-evaluative Threat on Neural Representations of Self
Authors: Hughes Brent L. , Beer Jennifer S.
Published: December 2012

One of the most robust ways that people protect themselves from social-evaluative threat is by emphasizing the desirability of their personal characteristics, yet the neural underpinnings of this fundamental process are unknown. The current fMRI study addresses this question by examining self-evaluations of desirability (in comparison with other people) as a response to threat. Participants judged how much personality traits described themselves in comparison with their average peer. These judgments were preceded by threatening or nonthreatening social-evaluative feedback. Self-evaluations made in response to threat significantly increased activation in a number of regions including the OFC, medial pFC, lateral pFC, amygdala, and insula. Individual differences in the extent to which threat increased desirability were significantly correlated with medial OFC activity. This is the first study to examine the neural associations of a fundamental self-protection strategy: responding to threat by emphasizing the self's desirability. Although neural research has separately examined self-evaluation processes from the regulation of social-evaluative threat, little is known about the interplay between the two. The findings build on this previous research by showing that regions, often associated with self-evaluation, are modulated by the degree to which people respond to threat by emphasizing their own desirability.
Variability and Predictability of a Three-Dimensional Hurricane in Statistical Equilibrium
Authors: Brown Bonnie R. , Hakim Gregory J.
Published: December 2012

The internal variability and predictability of idealized three-dimensional hurricanes is investigated using 100-day-long, statistically steady simulations in a compressible, nonhydrostatic, cloud-resolving model. The equilibrium solution is free of the confounding effects of initial conditions and environmental variability in order to isolate the “intrinsic” characteristics of the hurricane.

The variance of the axisymmetric tangential velocity is dominated by two patterns: one characterized by a radial shift of the maximum wind, and the other by intensity modulation at the radius of maximum wind. These patterns are associated with convectively coupled bands of anomalous wind speed that propagate inward from large radii with a period of roughly 5 days, the strongest of which is associated with an eyewall replacement cycle. The asymmetric tangential wind is strongest radially inward of the radius of maximum wind. On average, asymmetries decelerate the azimuthal-mean tangential wind at the radius of maximum wind and accelerate it along the inner edge of eyewall.

Predictability of axisymmetric storm structure is measured through the autocorrelation e-folding time and linear inverse modeling. Results from both methods reveal an intrinsic predictability time scale of about 2 days. The predictability and variability of the axisymmetric storm structure are consistent with recently obtained results from idealized axisymmetric hurricane modeling.
Evaluation of the CLM4 Lake Model at a Large and Shallow Freshwater Lake*
Authors: Deng Bin , Liu Shoudong , Xiao Wei , Wang Wei , Jin Jiming , Lee Xuhui
Published: December 2012

Models of lake physical processes provide the lower flux boundary conditions for numerical predictions of weather and climate in lake basins. So far, there have been few studies on evaluating lake model performance at the diurnal time scale and against flux observations. The goal of this paper is to evaluate the National Center for Atmospheric Research Community Land Model version 4–Lake, Ice, Snow and Sediment Simulator using the eddy covariance and water temperature data obtained at a subtropical freshwater lake, Lake Taihu, in China. Both observations and model simulations reveal that convective overturning was commonplace at night and timed when water switched from being statically stable to being unstable. By reducing the water thermal diffusivity to 2% of the value calculated with the Henderson–Sellers parameterization, the model was able to reproduce the observed diurnal variations in water surface temperature and in sensible and latent heat fluxes. The small diffusivity suggests that the drag force of the sediment layer in this large (2500 km2) and shallow (2-m depth) lake may be strong, preventing unresolved vertical motions and suppressing wind-induced turbulence. Model results show that a large fraction of the incoming solar radiation energy was stored in the water during the daytime, and the stored energy was diffused upward at night to sustain sensible and latent heat fluxes to the atmosphere. Such a lake–atmosphere energy exchange modulated the local climate at the daily scale in this shallow lake, which is not seen in deep lakes where dominant lake–atmosphere interactions often occur at the seasonal scale.
Public policies, interfaith associations and religious minorities: a new policy paradigm? Evidence from the case of Barcelona
Authors: Griera Mar
Published: December 2012

Religious diversity is posing new and urgent challenges to local authorities and there is no solid foundation of expertise in dealing with this issue at the local level. In some European cities, interfaith platforms are providing local authorities with new governance tools to cope with the challenges of religious diversity and are generating new ways of framing and representing religion in the public sphere. The author takes the city of Barcelona as a case study with the aim of exploring the emergence of a new model for dealing with religious minority issues that goes beyond State–Church relations and the political legacies in this area.
The national Church as a historical form of Church-type. Elements of a configurative theorization
Authors: Turcotte Paul-André
Published: December 2012

The national Church is characterized by a concentric synthesis of religion, nation, culture and society, in alliance with other social authorities. The socio-religious body it constitutes presents features similar to those of a system’s organic whole. Its composition may be described as plural and animated by an esprit de corps. The regulatory social codes structuring its varied social relations contribute to establishing the bond of reciprocity in the distinction. The various mediations implemented in this sense are aimed at periodically revitalizing a tradition in order to maintain an ecclesial body that is at the same time social; all the more so as the national Church is at odds with the conditions of organized religion in contemporary society, its historical characteristics notwithstanding.
Trying Something New
Authors: Condon Barbara Backer
Published: December 2012

Trying something new is a universal living experience of health. Although trying something new frequently occurs in healthcare, its meaning has never explicitly been studied. Parse’s humanbecoming school of thought is the theoretical perspective for this study. The research question for this study is: What is the structure of the living experience of trying something new? The purpose of this study was to advance nursing science. Parse’s qualitative phenomenological-hermeneutic research method was used to guide this study. Participants were 8 men and 2 women, ages 29 to 65 who utilize an outpatient mental health facility in the Midwest. Data were collected with dialogical engagement. The major finding of the study is the structure: Trying something new is engaging in capricious exploitations with vacillating sentiments, as wistful contemplation surfaces with disparate affiliations.
Theoretical Foundations of Nursing Practice in Poland
Authors: Zarzycka Danuta , Dobrowolska Beata , ślusarska Barbara , Wrońska Irena , Cuber Tomasz , Pajnkihar Majda
Published: December 2012

This authors of this column explore the use of nursing theory in Poland. A quasi-experimental pretest-posttest study was conducted to explore what, if any nursing theories were used by nurses in Poland, and if an education program on nursing theory increased the use of nursing theory. The study found that while there were discrepancies between the nurses’ theoretical knowledge base and their use of these theories in practice, there is evidence of the use of the ideas and theory of Nightingale, Orem, and Henderson in nursing practice in Poland.
Peplau’s Theory of Interpersonal Relations
Authors: Senn Joanne F.
Published: December 2012

The author in this column highlights aspects of Peplau’s theory of interpersonal relations and its use both in emergency nursing and rural nursing. Long and Weinert identified the concepts of rural nursing. Some differences between Peplau’s theory and rural nursing can be identified through definitions of theory and conceptual models. Despite these differences, there are some common themes between both theories that are described and compared.
The Importance of Defining Theory in Nursing
Authors: Karnick Paula M.
Published: December 2012

Identifying what theory is and how nursing as a discipline defines it, is of concern. This lack of consensus proves especially daunting when disseminating knowledge not only to nurses but others as well. If the profession is not able to articulate the foundation of theory, it is in a sorry state. By reviewing and critiquing the invited column perhaps nurses may establish what a theory is and how theory should be utilized in nursing. In order to differentiate theory from concepts, it is critical that theory developers are consistent.
Redeeming Beauty? Christa and the Displacement of Women’s Bodies in Theological Aesthetic Discourses
Authors: Vasko Elisabeth
Published: December 2012

This article adopts Edwina Sandys’ Christa as a hermeneutical lens through which to expose new dimensions about the interplay between aesthetics and redemption in the Christian tradition. Contemporary theological aesthetic discourses have ignored ugliness and its causes, especially the patriarchal ways in which Christian tradition has been used to sanctify violence against women. The issue of gender injustice takes on a heightened significance in light of recent claims surrounding the beauty of the cross. As a subversive aesthetic feminist representation, Christa exposes the patriarchal dimensions of such constructions and calls for a new vision of aesthetics – one that begins with women’s experiences of suffering and salvation.
Multilateral development banks and socially responsible investments – the case of tobacco
Authors: Lal Pranay
Published: December 2012

Globally, tobacco kills more people than HIV-related conditions or AIDS, tuberculosis and malaria combined. In 1991, The World Bank, the world’s largest lender, pledged that it would no longer support tobacco-related projects. It was expected that other financial investors would follow, but most did not respond to this call. As a result, several financial institutions continue to invest in tobacco and fuel an epidemic to an unprecedented scale. Using tobacco as a case in point, this review highlights the continuing investments among financial institutions which do not conform to ‘socially responsible investments’ and calls for monitoring and reporting such unethical practices. The paper also underscores the need to harmonise the numerous criteria, principles and voluntary codes that govern socially responsible investing and ensure that financial institutions comply with them.
Should pain be on the health promotion agenda?
Authors: Johnson Mark I. , Dixey Rachael
Published: December 2012

Opportunities and liberties available to individuals allow them to function as full human beings and this should include freedom from persistent pain. Pain seems to be a downstream phenomenon which tells us that something has gone wrong. Health promoters, with their upstream perspective, should be concerned with pain. In this commentary we discuss conceptual issues concerning ‘health’ as the absence of pain, issues of health justice and issues of health education to raise awareness of pain. We suggest that pain should appear on the health promotion agenda and conclude that more awareness is needed in the epistemic health promotion community of physical pain as a factor in the distribution of health and well-being. We argue the need for more research on the extent to which pain follows the social gradient in the same way that other health inequalities do.
A Retrospective Chart Review of 10 Hz Versus 20 Hz Repetitive Transcranial Magnetic Stimulation for Depression
Authors: DeBlasio Kristie L. , Tendler Aron
Published: December 2012

We performed a retrospective chart review to examine the progress of patients with depression who received different frequencies of repetitive transcranial magnetic stimulation (rTMS) delivered to the left dorsolateral prefrontal cortex (DLPFC). rTMS is a safe and effective alternative treatment for patients with various psychological and medical conditions. During treatment, a coil delivering a time-varying magnetic pulse placed over the scalp penetrates the skull, resulting in clinical improvement. There were 47 patients and three distinct treatment groups found: 10 Hz, 20 Hz, and a separate group who received both frequencies (10/20 Hz). The primary outcome indicator was the difference in Beck Depression Inventory–II (BDI-II) scores. Secondary outcomes included categorical indicators of remission, response, and partial response rates as assessed with the BDI-II. In all 3 groups, the majority of patients had depression that remitted, with the highest rate occurring in the 20 Hz group. There were similar response rates in the 10 Hz and 20 Hz groups. There were no patients in the 10/20 Hz group whose depression responded and the highest partial response and nonresponse rates occurred in this group. Although within-group differences were significant from baseline to end of treatment, there were no between-group differences.
Some new fractional q-integral Grüss-type inequalities and other inequalities
Authors: Zhu Chaowu , Yang Wengui , Zhao Qingbo
Published: December 2012

In this paper, we employ a fractional q-integral on the specific time scale,

T

t
0

=
{
t
:
t
=

t
0

q
n

,
n
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}
∪
{
0
}

, where

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0

∈
R

and

0
<
q
<
1

, to establish some new fractional q-integral Grüss-type inequalities by using one or two fractional parameters. Furthermore, other fractional q-integral inequalities are also obtained.

MSC:26D10, 26A33.
Insights into the regulation of human CNV-miRNAs from the view of their target genes
Authors: Wu Xudong , Zhang Dinglin , Li Guohui
Published: December 2012

Background

microRNAs (miRNAs) represent a class of small (typically 22 nucleotides in length) non-coding RNAs that can degrade their target mRNAs or block their translation. Recent research showed that copy number alterations of miRNAs and their target genes are highly prevalent in cancers; however, the evolutionary and biological functions of naturally existing copy number variable miRNAs (CNV-miRNAs) among individuals have not been studied extensively throughout the genome.

Results

In this study, we comprehensively analyzed the properties of genes regulated by CNV-miRNAs, and found that CNV-miRNAs tend to target a higher average number of genes and prefer to synergistically regulate the same genes; further, the targets of CNV-miRNAs tend to have higher variability of expression within and between populations. Finally, we found the targets of CNV-miRNAs are more likely to be differentially expressed among tissues and developmental stages, and participate in a wide range of cellular responses.

Conclusions

Our analyses of CNV-miRNAs provide new insights into the impact of copy number variations on miRNA-mediated post-transcriptional networks. The deeper interpretation of patterns of gene expression variation and the functional characterization of CNV-miRNAs will help to broaden the current understanding of the molecular basis of human phenotypic diversity.
GLAD4U: deriving and prioritizing gene lists from PubMed literature
Authors: Jourquin Jérôme , Duncan Dexter , Shi Zhiao , Zhang Bing
Published: December 2012

Background

Answering questions such as "Which genes are related to breast cancer?" usually requires retrieving relevant publications through the PubMed search engine, reading these publications, and creating gene lists. This process is not only time-consuming, but also prone to errors.

Results

We report GLAD4U (Gene List Automatically Derived For You), a new, free web-based gene retrieval and prioritization tool. GLAD4U takes advantage of existing resources of the NCBI to ensure computational efficiency. The quality of gene lists created by GLAD4U for three Gene Ontology (GO) terms and three disease terms was assessed using corresponding "gold standard" lists curated in public databases. For all queries, GLAD4U gene lists showed very high recall but low precision, leading to low F-measure. As a comparison, EBIMed's recall was consistently lower than GLAD4U, but its precision was higher. To present the most relevant genes at the top of a list, we studied two prioritization methods based on publication count and the hypergeometric test, and compared the ranked lists and those generated by EBIMed to the gold standards. Both GLAD4U methods outperformed EBIMed for all queries based on a variety of quality metrics. Moreover, the hypergeometric method allowed for a better performance by thresholding genes with low scores. In addition, manual examination suggests that many false-positives could be explained by the incompleteness of the gold standards. The GLAD4U user interface accepts any valid queries for PubMed, and its output page displays the ranked gene list and information associated with each gene, chronologically-ordered supporting publications, along with a summary of the run and links for file export and functional enrichment and protein interaction network analysis.

Conclusions

GLAD4U has a high overall recall. Although precision is generally low, the prioritization methods successfully rank truly relevant genes at the top of the lists to facilitate efficient browsing. GLAD4U is simple to use, and its interface can be found at: http://bioinfo.vanderbilt.edu/glad4u.
Microarray analysis of gene expression profiles in ripening pineapple fruits
Authors: Koia Jonni H , Moyle Richard L , Botella Jose R
Published: December 2012

Background

Pineapple (Ananas comosus) is a tropical fruit crop of significant commercial importance. Although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. Understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. In this study we developed a 9277 element pineapple microarray and used it to profile gene expression changes that occur during pineapple fruit ripening.

Results

Microarray analyses identified 271 unique cDNAs differentially expressed at least 1.5-fold between the mature green and mature yellow stages of pineapple fruit ripening. Among these 271 sequences, 184 share significant homology with genes encoding proteins of known function, 53 share homology with genes encoding proteins of unknown function and 34 share no significant homology with any database accession. Of the 237 pineapple sequences with homologs, 160 were up-regulated and 77 were down-regulated during pineapple fruit ripening. DAVID Functional Annotation Cluster (FAC) analysis of all 237 sequences with homologs revealed confident enrichment scores for redox activity, organic acid metabolism, metalloenzyme activity, glycolysis, vitamin C biosynthesis, antioxidant activity and cysteine peptidase activity, indicating the functional significance and importance of these processes and pathways during pineapple fruit development. Quantitative real-time PCR analysis validated the microarray expression results for nine out of ten genes tested.

Conclusions

This is the first report of a microarray based gene expression study undertaken in pineapple. Our bioinformatic analyses of the transcript profiles have identified a number of genes, processes and pathways with putative involvement in the pineapple fruit ripening process. This study extends our knowledge of the molecular basis of pineapple fruit ripening and non-climacteric fruit ripening in general.
Can treatment with Cocculine improve the control of chemotherapy-induced emesis in early breast cancer patients? A randomized, multi-centered, double-blind, placebo-controlled Phase III trial
Authors: Pérol David , Provençal Jocelyne , Hardy-Bessard Anne-claire , Coeffic David , Jacquin Jean-Phillipe , Agostini Cécile , Bachelot Thomas , Guastalla Jean-Paul , Pivot Xavier , Martin Jean-Pierre , Bajard Agathe , Ray-Coquard Isabelle
Published: December 2012

Background

Chemotherapy induced nausea and vomiting (CINV) remains a major problem that seriously impairs the quality of life (QoL) in cancer patients receiving chemotherapy regimens. Complementary medicines, including homeopathy, are used by many patients with cancer, usually alongside with conventional treatment. A randomized, placebo-controlled Phase III study was conducted to evaluate the efficacy of a complex homeopathic medicine, Cocculine, in the control of CINV in non-metastatic breast cancer patients treated by standard chemotherapy regimens.

Methods

Chemotherapy-naïve patients with non-metastatic breast cancer scheduled to receive 6 cycles of chemotherapy including at least three initial cycles of FAC 50, FEC 100 or TAC were randomized to receive standard anti-emetic treatment plus either a complex homeopathic remedy (Cocculine, registered in France for treatment of nausea and travel sickness) or the matching placebo (NCT00409071 clinicaltrials.gov). The primary endpoint was nausea score measured after the 1st chemotherapy course using the FLIE questionnaire (Functional Living Index for Emesis) with 5-day recall. Secondary endpoints were: vomiting measured by the FLIE score, nausea and vomiting measured by patient self-evaluation (EVA) and investigator recording (NCI-CTC AE V3.0) and treatment compliance.

Results

From September 2005 to January 2008, 431 patients were randomized: 214 to Cocculine (C) and 217 to placebo (P). Patient characteristics were well-balanced between the 2 arms. Overall, compliance to study treatments was excellent and similar between the 2 arms. A total of 205 patients (50.9%; 103 patients in the placebo and 102 in the homeopathy arms) had nausea FLIE scores > 6 indicative of no impact of nausea on quality of life during the 1st chemotherapy course. There was no difference between the 2 arms when primary endpoint analysis was performed by chemotherapy stratum; or in the subgroup of patients with susceptibility to nausea and vomiting before inclusion. In addition, nausea, vomiting and global emesis FLIE scores were not statistically different at any time between the two study arms. The frequencies of severe (Grade ≥ 2) nausea and vomiting were low in our study (nausea: P: 17.6% vs C: 15.7%, p=0.62; vomiting: P: 10.8% vs C: 12.0%, p=0.72 during the first course).

Conclusion

This double-blinded, placebo-controlled, randomised Phase III study showed that adding a complex homeopathic medicine (Cocculine) to standard anti-emetic prophylaxis does not improve the control of CINV in early breast cancer patients.
An epidemiologic study of factors associated with nasal septum deviation by computed tomography scan: a cross sectional study
Authors: Mohebbi Alireza , Ahmadi Aslan , Etemadi Maryam , Safdarian Manouchehr , Ghourchian Shadi
Published: December 2012

Background

Sinusitis is an inflammation of the paranasal sinuses that can be caused by anatomic variations of the nasal cavity and paranasal sinuses. In this study we aimed to find the relationship between sinusitis and septal deviation (SeD) and concha bullosa.

Methods

Two trained resident of ENT evaluated sinus CT scans of 463 cases presenting with nasal obstruction or chronic sinusitis symptoms from April 2011 to December 2011. CT scans were checked for the presence of conchae bullosa and the degree of septal deviation. The severity of sinusitis was evaluated according to the Lund Mackay criteria. The frequency of patients with different degrees of SeD and different grades of chronic sinusitis were studied.

Results

Of 463 cases, 47% had septal deviation. Concha bullosa was seen in 16.8% of the patients in the left side and 27.6% of them in the right side. There was no significant relationship between the presence of concha bullosa and the severity of sinusitis. Also the P value of analytical tests between the severity of sinusitis, osteomeatal involvement and the degree of septal deviation was not significant. Analysis of the relationship between the presence of SeD (either to right or left) and the severity of sinusitis in different sinuses revealed no significant P value.

Conclusions

By this study, the relationship between concha bullosa in osteomeatal complex and the severity of sinusitis was not cleared. No relationship was found between the severity of sinusitis, osteomeatal involvement and the degree of septal deviation. Also SeD (either to right or left) was not found to be associated with the severity of sinusitis in different sinuses.
MGMT promoter methylation status and MGMT and CD133 immunohistochemical expression as prognostic markers in glioblastoma patients treated with temozolomide plus radiotherapy
Authors: Melguizo Consolación , Prados Jose , González Beatriz , Ortiz Raul , Concha Angel , Alvarez Pablo Juan , Madeddu Roberto , Perazzoli Gloria , Oliver Jaime Antonio , López Rodrigo , Rodríguez-Serrano Fernando , Aránega Antonia
Published: December 2012

Background

The CD133 antigen is a marker of radio- and chemo-resistant stem cell populations in glioblastoma (GBM). The O6-methylguanine DNA methyltransferase (MGMT) enzyme is related with temozolomide (TMZ) resistance. Our propose is to analyze the prognostic significance of the CD133 antigen and promoter methylation and protein expression of MGMT in a homogenous group of GBM patients uniformly treated with radiotherapy and TMZ. The possible connection between these GBM markers was also investigated.

Methods

Seventy-eight patients with GBM treated with radiotherapy combined with concomitant and adjuvant TMZ were analyzed for MGMT and CD133. MGMT gene promoter methylation was determined by methylation-specific polymerase chain reaction after bisulfite treatment. MGMT and CD133 expression was assessed immunohistochemically using an automatic quantification system. Overall and progression-free survival was calculated according to the Kaplan–Meier method.

Results

The MGMT gene promoter was found to be methylated in 34 patients (44.7%) and unmethylated in 42 patients (55.3%). A significant correlation was observed between MGMT promoter methylation and patients’ survival. Among the unmethylated tumors, 52.4% showed low expression of MGMT and 47.6% showed high-expression. Among methylated tumors, 58.8% showed low-expression of MGMT and 41.2% showed high-expression. No correlation was found between MGMT promoter methylation and MGMT expression, or MGMT expression and survival. In contrast with recent results, CD133 expression was not a predictive marker in GBM patients. Analyses of possible correlation between CD133 expression and MGMT protein expression or MGMT promoter methylation were negative.

Conclusions

Our results support the hypothesis that MGMT promoter methylation status but not MGMT expression may be a predictive biomarker in the treatment of patients with GBM. In addition, CD133 should not be used for prognostic evaluation of these patients. Future studies will be necessary to determine its clinical utility.
Widely linear Markov signals
Authors: Espinosa-Pulido Juan A , Navarro-Moreno Jesús , Fernández-Alcalá Rosa M , Ruiz-Molina Juan C
Published: December 2012

The insufficiency to guarantee the existence of a state-space representation of the classical wide-sense Markov condition for improper complex-valued signals is shown and a generalization is suggested. New characterizations for wide-sense Markov signals which are based either on second-order properties or on state-space representations are studied in a widely linear setting. Moreover, the correlation structure of such signals is revealed and interesting results on modeling in both the forwards and backwards time directions are proved. As an application we give some recursive estimation algorithms obtained from the Kalman filter. The performance of the proposed results is illustrated in a numerical example in the areas of estimation and simulation.
Soluble microbial products (SMPs) release in activated sludge systems: a review
Authors: Azami Hamed , Sarrafzadeh Mohammad Hossein , Mehrnia Mohammad Reza
Published: December 2012

This review discusses the characterization, production and implications of soluble microbial products (SMPs) in biological wastewater treatment. The precise definition of SMPs is open to talk about, but is currently regarded as “the pool of organic compounds that are released into solution from substrate metabolism and biomass decay”'. Some of the SMPs have been identified as humic acids, polysaccharides, proteins, amino acids, antibiotics, extracellular enzymes and structural components of cells and products of energy metabolism. They adversely affect the kinetic activity, flocculating and settling properties of sludge. This review outlines some important findings with regard to biodegradability and treatability of SMPs and also the effect of process parameters on their production. As SMPs are produced during biological treatment process, their trace amounts normally remain in the effluent that defines the highest COD removal efficiency. Their presence in effluent represents a high potential risk of toxic by-product formation during chlorine disinfection. Studies have indicated that among all wastewater post-treatment processes, the adsorption by granular activated carbon combined with biologically induced degradation is the most effective method for removal of SMPs. However, it may be concludes that the knowledge regarding SMPs is still under progress and more work is required to fully understand their contribution to the treatment process.
Inflammatory monocytes and the pathogenesis of viral encephalitis
Authors: Terry Rachael L , Getts Daniel R , Deffrasnes Celine , van Vreden Caryn , Campbell Iain L , King Nicholas JC
Published: December 2012

Monocytes are a heterogeneous population of bone marrow-derived cells that are recruited to sites of infection and inflammation in many models of human diseases, including those of the central nervous system (CNS). Ly6Chi/CCR2hi inflammatory monocytes have been identified as the circulating precursors of brain macrophages, dendritic cells and arguably microglia in experimental autoimmune encephalomyelitis; Alzheimer’s disease; stroke; and more recently in CNS infection caused by Herpes simplex virus, murine hepatitis virus, Theiler’s murine encephalomyelitis virus, Japanese encephalitis virus and West Nile virus. The precise differentiation pathways and functions of inflammatory monocyte-derived populations in the inflamed CNS remains a contentious issue, especially in regard to the existence of monocyte-derived microglia. Furthermore, the contributions of monocyte-derived subsets to viral clearance and immunopathology are not well-defined. Thus, understanding the pathways through which inflammatory monocytes migrate to the brain and their functional capacity within the CNS is critical to inform future therapeutic strategies. This review discusses some of the key aspects of inflammatory monocyte trafficking to the brain and addresses the role of these cells in viral encephalitis.
ABCG2/V-ATPase was associated with the drug resistance and tumor metastasis of esophageal squamous cancer cells
Authors: Huang Lijun , Lu Qiang , Han Yong , Li Zhe , Zhang Zhipei , Li Xiaofei
Published: December 2012

Background

ATP-binding cassette sub-family G member 2 (ABCG2) is a protein that in humans is encoded by the ABCG2 gene. ABCG2 participates in efflux of many chemotherapeutic agents. ABCG2 is often expressed in hematopoietic progenitor or stem cells. Vacuolar-H + −ATPase (V-ATPase) plays a key role in adjusting and maintaining intracellular pH and in regulating the drug tolerance of cells. The TNM Classification of Malignant Tumours (TNM) is a cancer staging system that describes the extent of cancer in a patient’s body. In this study, the expression of ABCG2 and V-ATPase in esophageal squamous cancer cells was detected.

Methods

Immunohistochemistry staining and Immunofluorescence double staining were used to detect the expression of ABCG2 and V-ATPase in in 66 cases of esophageal squamous cancer cells. Associations and differences in expression of ABCG2 with that of V-ATPase were analyzed.

Results

Positive staining patterns for both ABCG2 (66.67%) and V-ATPase (68.18%) were located mainly in the plasma membrane and cytoplasm. Marked differences in expression were also shown (P < 0.001) among 3 groups of pathological grades and TNM stages in these carcinomas. Marked differences were also found for ABCG2 expression between the two groups in the pathological grades and in the TNM staging groups (P < 0.01), but not between the αb and βgroups. V-ATPase expression was statistically significant between the 2 groups in the pathological grades and TNM stages (P < 0.05). This was not evident between α and β groups of pathological grades or between αb and βof the TNM stages. Marked differences in expression of ABCG2 and V-ATPase were found between metastatic and non-metastatic groups in the same carcinomas (P < 0.0001). There was also a clear correlation between the expression of ABCG2 and V-ATPase (P ≤ 0.001) in the various groups of pathological grades and TNM stages.

Conclusions

Both ABCG2 and V-ATPase were over-expressed in esophageal squamous cancer cells. Their expression was associated with pathological grade, TNM stage and tumor metastasis in esophageal squamous cancer cells, suggesting interaction relationship between them. ABCG2 and V-ATPase expression may be strongly associated with drug resistance and tumor metastasis.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3823783918433897
Investigation of the change in marker geometry during respiration motion: a preliminary study for dynamic-multi-leaf real-time tumor tracking
Authors: Yamazaki Rie , Nishioka Seiko , Date Hiroyuki , Shirato Hiroki , Koike Takao , Nishioka Takeshi
Published: December 2012

Background

The use of stereotactic body radiotherapy (SBRT) is rapidly increasing. Presently, the most accurate method uses fiducial markers implanted near the tumor. A shortcoming of this method is that the beams turn off during the majority of the respiratory cycle, resulting in a prolonged treatment time. Recent advances in collimation technology have enabled continuous irradiation to a moving tumor. However, the lung is a dynamic organ characterized by inhalation exhalation cycles, during which marker/tumor geometry may change (i.e., misalignment), resulting in under-dosing to the tumor.

Findings

Eight patients with lung cancer who were candidates for stereotactic radiotherapy were examined with 4D high-resolution CT. As a marker surrogate, virtual bronchoscopy using the pulmonary artery (VBPA) was conducted. To detect possible marker/tumor misalignment during the respiration cycle, the distance between the peripheral bronchus, where a marker could be implanted, and the center of gravity of a tumor were calculated for each respiratory phase. When the respiration cycle was divided into 10 phases, the median value was significantly larger for the 30%-70% respiratory phases compared to that for the 10% respiratory phase (P<0.05, Mann–Whitney U-test).

Conclusions

These results demonstrate that physiological aspect must be considered when continuous tumor tracking is applied to a moving tumor. To minimize an “additional” internal target volume (ITV) margin, a marker should be placed approximately 2.5 cm from the tumor.