bluuebunny/crossref_metadata_2025 huggingface dataset:
SIBERIAN PAPERWORK AND CARTOGRAPHIC SOURCES OF THE TURN OF THE 17th–18th CENTURIES: THE PHENOMENON OF ‘RECOGNISING’ OF NEW TERRITORIES IN THE EMPIRE
Authors: Kabakova N.V. , Korusenko S.N.
Published: January 2020

The present article is aimed at estimating the informative value of the Patrol Book of the Tara District (1701) and the Chorographic Drawing Book of Siberia by Semen U. Remezov. Drawing on the comparative analysis of these documents, the authors consider their presentation of the south of Western Siberia through the settlement system of the Russian and Tatar. The study covers late 17th — early 18th centuries, which is associated with the datings of the studied sources. The Patrol Book was compiled by Ivan R. Kachanov, with the patrol having been ordered by Peter I. The Patrol Book contains statistical and descriptive material, includes information on settle-ments, their inhabitants, as well as various geographical features. Also by the order of Peter I, Semen Remezov created the Chorographic Drawing Book in 1697–1711. It included known data on Siberia and adjacent territories. This source is organised in accordance with the river routes. The Tara District of the Tobolsk Governorate is de-picted on pages 84–93 and 107. The maps contain information similar to that found in the Patrol Book, which provides the opportunity to perform a comparative analysis of these two documents. A total of 48 Russian and
51 Tatar settlements are described in the Patrol Book. The Chorographic Book depicts 65 Russian settlements, three small forts (ostrogs), 79 yurts, 5 towns and 8 Tatarian volosts. The difference in numbers was due to the fact that Ivan Kachanov was to note places of residence, places of tax collection, as well as places to which the authorities allocated pay for service-men. During this period, the population of the Tara District developed new lands and founded new temporary settlements, some of which years later turned into permanent ones. Semen Remezov recorded these settlements, so their number on the map is greater than in Patrol Book. The comparison of the studied documents revealed the inconsistency in the names of many settlements. A comparative analysis and comparison of the content of the sources helped determine settlements having similar names and identify identical settlements having different names. The reason for the unestablished names is that some settlements were named after the surnames of first inhabitants; others were named after geographical objects or other inhabi-tants. For Siberia, both sources, on the one hand, were a sensory, somewhat illusory phenomenon, and on the other, they constituted an experimental perception of the newly included territories.
NEW DATA OBTAINED ON THE ARCHAEOZOOLOGY OF THE ALAKUL POPULATION FROM THE MIDDLE TOBOL AREA DRAWING ON THE EXCAVATION MATERIALS FROM THE ZOLOTOYE 1 SETTLEMENT
Authors: Kostomarov V.M. , Novikov I.K. , Kisagulov A.V.
Published: January 2020

The article presents the results of a taxonomic study of the archaeozoological collection from the Zolotoye 1 settlement. The settlement is located in the steppe zone of the Tobol-Ishim interfluve (the Polovinsky District of the Kurgan Region). A significant part of artefacts, including bone remains, belong to the Alakul culture of the Late Bronze Age (17th–16th centuries BC). A small collection (a total of 6 fragmented vessels) attributed to the Alek-seyevka-Sargary culture was also identified. The relevance of this work is determined by the fact that data on the species composition of Alakul archaeozoological collections are predominantly obtained from necropolises, whereas economic characteristics are primarily reflected by materials from the settlements. The study in question was conducted using the paleozoological method. The taxonomic affiliation of bones was determined using the reference collection of skeletons from the Zoological Museum of the Institute of Plant and Animal Ecology UB RAS along with corresponding atlases. The conclusion about the taxonomic affiliation of fossil remains was based on the similarities in composition and size between the morphological structures of bones. The age of the indi-viduals was determined by the degree of tooth abrasion and by the attachment of the pineal gland. The studied osteological collection includes 2783 items. In order to define the features of its occurrence considering species composition, a planigraphic analysis was performed. To this end, we used data collected from a digital total sta-tion and field inventories. As a result, it was found that the bone remains belong mainly to domestic animals
(99.5 %). Cattle bones (47 %) predominate, followed by the bones of small cattle (34 %) and horses (18 %). Jud-ging by age characteristics, cattle were kept for the production of milk and meat. The remains of wild animals are scarce (0.5 %). They include commercial species (elk, hare, waterfowl), which indicates that the Alakul population was engaged in hunting. The comparison of domestic and wild animals, the composition of the herd from the Zolotoye 1 settlement located in the interfluve area with the archaeozoological collections of the Late Bronze Age from the forest-steppe Trans-Urals revealed their similarity, first of all, with Alakul materials originating from the layer of settlements confined to river systems. This fact reflects the general line of development in livestock breeding of the period under consideration, which suggests that the carriers of the Alakul culture developed stable forms of adaptation to different living conditions.
Stranačka politika u Vukovaru 1990-1991.
Authors: Filipović Vladimir
Published: January 2020

U članku se analizira lokalna politika u općini Vukovar od pluralizacije po-litičkog života u Hrvatskoj početkom 1990. do sloma demokratski izabranih lokalnih političkih institucija u svibnju 1991. To razmjerno kratko razdoblje obilježila je snažna politička polarizacija između dviju najbrojnijih nacionalnih zajednica, Hrvata i Srba, u općini. U tom su procesu tri političke stranke – savez komunista Hrvatske-stranka demokratskih promjena, Hrvatska demokratska zajednica i Srpska demokratska stranka – bile glavni akteri nacionalne mobilizacije i političkog života u Vukovaru.
Justice and the politics of capabilities
Authors: Cvijanović Hrvoje
Published: January 2020

Linking the idea of justice with human dignity through the 'politics of capabilities' is a recent theoretical project advanced by Amartya Sen and Martha Nussbaum and inspired by the shortcomings of Rawls's understanding of justice. Rawls's view on the redistribution of resources or primary goods has nothing to say about someone's capabilities to use these goods, so the idea of capabilities becomes central since it is focused on the way of life a person has a reason to value. The article discusses Sen and Nussbaum's development of the capabilities approach and their criticism of Rawls's fundamental premises about justice. Although the capabilities approach attempts to rectify injustices that Rawls failed to address properly, there are limitations of that approach as well. At the end, it is shown that capabilities are valued in relation to their contribution to the system of production – having more capabilities enhances someone's socio-economic position within the given structure – but this does not question the existing power relations and the very structure that perpetuate inequalities. Hence, the author agrees with the line of criticism that invokes the issue of power relations provided by Vicente Navarro, yet extending this criticism to Rawls's theory of justice for not fundamentally questioning the power relations inherent in the institutions reproducing social injustices.
Assessment of the Development of Professional Skills in University Students: Sustainability and Serious Games
Authors: Peña Miguel Noemi , Corral Lage Javier , Mata Galindez Ana
Published: January 2020

This study assesses the development of professional skills in university students using serious games (SG), from a sustainability perspective. The Sustainable Development Goals (SDGs) were set by the United Nations’ 2030 Agenda for Sustainable Development. Universities are strategic agents in the transformation process towards sustainability. This way, they should be committed to promoting such sustainable values in the students through curricular sustainability, implementing active methodologies and SG for that purpose. Transversal skills are essential for the development of future graduates. The objective of this study was to assess which professional skills should be developed through the SG called The Island, to improve the degree of student satisfaction with the incorporation of a sustainable curriculum. The data were obtained using a questionnaire, and then analysed using linear regression models, with their inference estimated through the goodness of fit and ANOVA. The first results indicated that the implementation of the SG promoted a strengthening of the students' sustainable curriculum through the development of those skills. It was concluded that the key to success in education for sustainable development is improving the development of strategic thinking, collaborative thinking, and self-awareness, in addition to encouraging systemic, critical, and problem-solving thinking.
Реализация полностью консервативной лагранжево-эйлеровой схемы для двумерных задач магнитной газодинамики
Authors: Круковский Александр Юрьевич , Krukovskii Aleksandr Yur'evich , Гасилов Владимир Анатольевич , Gasilov Vladimir Anatol'evich , Повещенко Юрий Андреевич , Poveschenko Yurii Andreevich , Шарова Юлия Сергеевна , Sharova Yuliya Sergeevna , Клочкова Людмила Викторовна , Klochkova Ludmila Viktorovna
Published: January 2020

Рассматривается алгоритм численного решения уравнений магнитной газодинамики (МГД), аппроксимированных полностью консервативной лагранжево-эйлеровой разностной схемой (ПКРС). Полная система уравнений динамики высокотемпературной среды решается с учетом кондуктивного (электронного, ионного) и лучистого переноса тепла. Этап расчета, относящийся к вычислениям на лагранжевой подвижной сетке, реализуется на основе неявных аппроксимаций. Соответствующие разностные уравнения решаются итерационным методом с последовательным учетом физических процессов. Получены оценки сходимости для различных комбинаций разностных уравнений, сгруппированных соответственно физическим процессам. Справедливость полученных оценок подтверждена в вычислительных экспериментах с модельными и прикладными задачами.
Fábulas a la luz de la liebre
Authors: Espinosa Germán
Published: January 2020

Este trabajo resucita en el viejo continente cinco de los artículos que Germán Espinosa (Cartagena de Indias, 1938 - Bogotá, 2007) publicó dentro de La liebre en la luna (1991), luego reeditados en el volumen I de sus Ensayos completos (Medellín, Fondo Editorial de la Universidad EAFIT): «Sobre la génesis y evolución del arte de fabular», «El ocioso trabajo de escribir», «La novela: de cara al siglo XXI», «La raíz expresionista de Juan Rulfo» y «El verdadero héroe de Bomarzo».
El crimen, la imagen de Colombia y la interpretación del presente en los cuentos de Germán Espinosa
Authors: Araújo Fontalvo Orlando
Published: January 2020

El crimen es una de las temáticas más significativas en la cuentística de Germán Espinosa. Para comprender la naturaleza de esta recurrencia hay que revisar cuidadosamente las breves ficciones en donde lo criminal se manifiesta con una fuerza inatajable, con un impulso sin tregua ni respiro, poniendo de manifiesto una sociedad anómica en su tejido más hondo y descarnado.
Literatura y sociedad en Colombia a través de Aitana (2007), de Germán Espinosa
Authors: Pineda Buitrago Sebastián
Published: January 2020

Pocos meses antes de morir, el escritor colombiano Germán Espinosa (1938-2007) publicó la última y más autobiográfica de sus novelas, Aitana (2007), en la que fusionó su literatura y su vida cotidiana en la Bogotá de la primera década del siglo XXI. Tanto el crescendo de horror y del suspenso como la invención esotérica de un brujo no restan realismo a Aitana, sino que más bien intensifican el pesimismo de Espinosa frente a la sociedad colombiana, cuya intelectualidad simpatiza en gran parte, según él, con la violencia en virtud de una actitud groseramente materialista. Aitana es el testamento literario de un prolífico novelista que se sintió a sí mismo “incomprendido”, fruto, por una parte, de su exceso verbal y erudito; y por otra, a causa de las precarias instituciones culturales de su país.
De las "Tardes entretenidas" (1625) a "La quinta de Laura" (1649): apuntes sobre la evolución narrativa de Castillo Solórzano
Authors: Grouzis Demory Christelle
Published: January 2020

Este artículo se propone reflexionar sobre la evolución narrativa de Alonso de Castillo Solórzano a través del análisis contrastado entre dos colecciones de novelas cortas -"Tardes entretenidas" (1625) y "La quinta de Laura" (1649)- que marcaron el principio y el final de una producción literaria abundante y variada. Nos interesaremos en particular por el marco narrativo en las dos obras, por los temas que se desarrollan en los relatos y por la escritura del autor..
“Ved que es hijo la víctima acusada”. Versiones españolas olvidadas de la muerte del príncipe don Carlos entre el XVIII y el XIX
Authors: Durán López Fernando
Published: January 2020

Uno de los principales ingredientes de la llamada Leyenda Negra fue la tiránica y cruel personalidad de Felipe II, a quien se atribuía la prisión y muerte de su hijo don Carlos, el Príncipe de Asturias. Una versión difundida desde Francia asociaba esa crisis a una presunta relación amorosa entre Carlos y la esposa de Felipe, Isabel de Valois. La transmisión de esa imagen en España fue más difícil, porque el ataque al rey estaba mezclado con el ataque al país y su orgullo nacional. Se analizan aquí, en el marco general de las versiones españolas del episodio, algunos testimonios de entre el XVIII y el XIX no considerados hasta el momento: principalmente la tragedia anónima e inédita "El Príncipe don Carlos", que concursó al certamen de tragedias convocado en 1798 por la Real Academia Española; y de modo secundario, el ensayo histórico publicado en italiano en 1815 por el jesuita expulso Mariano Llorente.
Teología y poesía en Unamuno: El Cristo de Velázquez
Authors: Fernández Gallardo Luis
Published: January 2020

La religiosidad de Unamuno, deudora del catolicismo tradicional y popular, del culto a las imágenes, está en el fondo de la inspiración de El Cristo de Velázquez. La elección de este cuadro supone una retractación de su predilección por los Cristos sangrantes y patéticos y eleva aquél a expresión de la religiosidad hispana. Unamuno no cultiva la ékphrasis: su contemplación no es estética, sino mística. Así, desarrolla una meditación cristológica mediante un rico mosaico de imágenes fundadas en el concepto exegético defigura. Éstas se ajustan a una estructura enumerativa, en forma de letanía, que Unamuno consideró como la más exquisita manifestación de la lírica. De este modo, explora los nomina Christi en audaz interpretación.
En busca de un destino cumplido: el padre Eusebio del Niño Jesús O.C.D. y su defensa de la voz pura de Santa Teresa
Authors: Correa Ramón Amelina
Published: January 2020

Se rescata aquí la figura del P. Eusebio del Niño Jesús (1888-1936), carmelita descalzo de formación intelectual y final muy trágico, que luchó denodadamente por reconducir lo que juzgaba excesos interpretativos del espiritismo acerca de la fundadora del Carmelo, para restablecer, en suma, la voz pura de Santa Teresa.
El legado literario de Antonio Gala: estado de la cuestión
Authors: Cobo Guijarro Clara , Padilla Mangas Ana
Published: January 2020

El presente artículo contiene la revisión de las principales áreas de estudio que se han desarrollado a lo largo de los últimos cincuenta años acerca de la obra literaria de Antonio Gala. Estos trabajos académicos han abordado las diversas facetas del autor como dramaturgo, novelista y poeta, pero también como guionista o articulista en los principales diarios nacionales. Así, en los diferentes apartados se evaluarán los aspectos más relevantes del legado literario del escritor cordobés en consonancia con la bibliografía derivada de su obra, elaborada por la comunidad académica nacional e internacional. Asimismo, completamos dicho estado de la cuestión con los desafíos y parcelas de discusión pendientes de ser abordados en el futuro.
Improved Estimation and Forecasting Through Residual-Based Model Error Quantification
Authors: Lu Minjie , Chen Yan
Published: January 2020

Owing to the complex nature of hydrocarbon reservoirs, the numerical model constructed by geoscientists is always a simplified version of reality: for example, it might lack resolution from discretization and lack accuracy in modeling some physical processes. This flaw in the model that causes mismatch between actual observations and simulated data when “perfect” model parameters are used as model inputs is known as “model error”. Even in a situation when the model is a perfect representation of reality, the inputs to the model are never completely known. During a typical model calibration procedure, only a subset of model inputs is adjusted to improve the agreement between model responses and historical data. The remaining model inputs that are not calibrated and are likely fixed at incorrect values result in model error in a similar manner as the imperfect model scenario.

Assimilation of data without accounting for model error can result in the incorrect adjustment to model parameters, the underestimation of prediction uncertainties, and bias in forecasts. In this paper, we investigate the benefit of recognizing and accounting for model error when an iterative ensemble smoother is used to assimilate production data. The correlated “total error” (a combination of model error and observation error) is estimated from the data residual after a standard history-matching using the Levenberg-Marquardt form of iterative ensemble smoother (LM-EnRML). This total error is then used in further data assimilations to improve the estimation of model parameters and quantification of prediction uncertainty. We first illustrate the method using a synthetic 2D five-spot example, where some model errors are deliberately introduced, and the results are closely examined against the known “true” model. Then, the Norne field case is used to further evaluate the method.

The Norne model has previously been history-matched using the LM-EnRML (Chen and Oliver 2014), where cell-by-cell properties (permeability, porosity, net-to-gross, vertical transmissibility) and parameters related to fault transmissibility, depths of water/oil contacts, and relative permeability function are adjusted to honor historical data. In this previous study, the authors highlighted the importance of including large amounts of model parameters, the proper use of localization, and heuristic adjustment of data noise to account for modeling error. In this paper, we improve the last aspect by quantitatively estimating model error using residual analysis.
Superiority of mucosal incision-assisted biopsy over ultrasound-guided fine needle aspiration biopsy to diagnose small gastric subepithelial lesions:A propencity score matching analysis
Authors: Minoda Yosuke , Chinen Takatoshi , Osoegawa Takashi , Itaba Soichi , Haraguchi Kazuhiro , Akiho Hirotada , Aso Akira , Sumida Yorinobu , Komori Keishi , Ogino Haruei , Ihara Eikichi , Ogawa Yoshihiro
Published: January 2020

Background Gastric subepithelial lesions, including gastrointestinal stromal tumors, are often found during routine gastroscopy. While endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) has been the gold standard for diagnosing gastric subepithelial lesions, alternative open biopsy procedures, such as mucosal incision-assisted biopsy (MIAB) has been reported useful. The aim of this study is to evaluate the efficacy of MIAB for the diagnosis of gastric SELs compared with EUS-FNAB. Methods We retrospectively analyzed medical records of 177 consecutive patients with gastric SELs who underwent either MIAB or EUS-FNAB at five hospitals in Japan between January 2010 and January 2018. Diagnostic yield, procedural time, and adverse event rates for the two procedures were evaluated before and after propensity-score matching. Results No major procedure-related adverse events were observed in either group. Both procedures yielded highly-accurate diagnoses once large enough samples were obtained; however, such successful sampling was more often accomplished by MIAB than by EUS-FNAB, especially for small SELs. As a result, MIAB provided better diagnostic yields for SELs smaller than 20-mm diameter. The diagnostic yields of both procedures were comparable for SELs larger than 20-mm diameter; however, MIAB required significantly longer procedural time (approximately 13 minutes) compared with EUS-FNAB. Conclusions Although MIAB required longer procedural time, it outperformed EUS-FNAB when diagnosing gastric SELs smaller than 20-mm diameter.
The effect of season and post-fire on habitat preferences of the endangered Swayne’s hartebeest (Alcelaphus buselaphus swaynei) in Maze National Park, Ethiopia
Authors: Tamrat Misganaw , Atickem Anagaw , Tsegaye Diress , Evangelista Paul , Bekele Afework , Stenseth Nils Christian
Published: January 2020

Background: The availability of preferred habitats determines the spatial and temporal distribution of herbivores in savanna ecosystems. Understanding habitat preference of a targeted wildlife species is crucial for developing effective conservation strategies. Habitat preference of large grazers in connection to grass height and post-fire effect has been debated for the last century. Here, we examined the effects of season, grass height and burning on the habitat preference on Swayne’s hartebeest (Alcelaphus buselaphus swaynei) in Maze National Park. Data for seasonal habitat selection were collected using both direct observation along established transect lines and pellet counting using permanently established plots. Every month, we measured grass height commonly preferred by Swayne’s hartebeest in grassland habitat. Starting from the first week of burning, we recorded the abundance of Swayne’s hartebeest in both burned and unburned grassland patches.
Results: From detected pellets, 94.3% were recorded in the grassland habitat indicating that other habitat types are less used despite their extensive cover > 50% of the Park. During wet and early dry seasons, Swayne’s hartebeest exclusively preferred grassland habitat. We found that 85.2% (n=1,079) and 85.3% (n=593) of individuals observed in areas with a grass height below 30 cm during wet and early-dry seasons, respectively; while 70.9% (n=2,288) preferred grass height below 30 cm during the dry season. The density of Swayne’s hartebeest in burned grassland area was higher than unburned grassland areas up to 150 days since burning. However, in unburned grassland areas, the density was initially low but showed increasing trend for consecutive days, reaching similar density with burned areas after 150 days since burning.
Conclusion: Swayne’s hartebeest exclusively preferred grassland habitat, particularly during wet and early-dry seasons, shortest available grass height in all seasons and were attracted to burned grassland areas. Our results suggested that fire played an important role in maintaining habitat quality in grassland, and that management should continue using controlled burning as a tool for the conservation of Swayne’s hartebeest. However, we remain cautious of our findings given the paucity of information regarding other confounding factors and the absence of long-term data on fire disturbance.
The association between individual perceptions of PM2.5 pollution and pulmonary function in Chinese middle-aged and elderly residents
Authors: Chen Qi , Zhang Jiayao , Xu Yan , Sun Hong , Ding Zhen
Published: January 2020

Background: PM2.5 pollution has become a major public health concern in urban China. Understanding the residents’ individual perceptions toward haze pollution is critical for policymaking and risk communication. However, the perceptions of middle-aged and elderly residents, who particularly vulnerable to haze pollution, are poorly understood. In this study, we aimed to explore their risk perception of haze pollution, and investigating its relationship with health status and pulmonary function parameters. Methods: A cross-sectional study of 400 randomly sampled individuals (aged 40 to 90 years) was conducted in Wuxi, a typical PM2.5-polluted city in Jiangsu Province, China (daily average PM2.5 concentration was 52.73 μg/m3 between 2015-2017). Each participant’s demographic and health information, individual perception and pulmonary function outcomes were collected, and linear models were employed to explore the relationship between personal characteristics and pulmonary function parameters and perception factors. Results: We found that the mean values for controllability (4.99 ± 2.78) and dread of risk to oneself (levels of fear for the haze-related harm to oneself) (6.90 ± 2.45) were the lowest and the highest values, respectively, in our study. Education and average family income were positively related with all individual perception factors, while age was negatively associated. A history of respiratory disease was positively associated with all individual perception factors except controllability. Significant positive associations were observed between PEF (coefficients ranged from 0.18 to 0.22) and FEF75% (coefficients ranged from 0.18 to 0.29) with a variety of individual perception factors. Conclusions: There was a lack of concern and knowledge, weak self-protection consciousness and a strong dread of PM2.5 pollution among the middle-aged and elderly residents in Wuxi. Their individual perceptions were associated with age, education levels, average family income, history of respiratory disease and pulmonary function indexes PEF and FEF75%. Our findings may help policymakers develop effective policies and communication strategies to mitigate the hazards of haze among older residents.
Effect of Porphyromonas gingivalis infection on gut dysbiosis and arthritis exacerbation in 1 mouse model
Authors: Hamamoto Yuta , Ouhara Kazuhisa , Munenaga Syuichi , Shoji Mikio , Ozawa Tatsuhiko , Hisatsune Jyunzo , Kado Isamu , Kajiya Mikihito , Matsuda Shinji , Kawai Toshihisa , Mizuno Noriyoshi , Fujita Tsuyoshi , Hirata Shintaro , Tanimoto Kotaro , Nakayama Koji , Kishi Hiroyuki , Sugiyama Eiji , Kurihara Hidemi
Published: January 2020

Background : Porphyromonas gingivalis (Pg) infection causes periodontal disease and is one of the causative bacteria of rheumatoid arthritis (RA) exacerbation. Gut microbiota dysbiosis has shown strong associations with systemic diseases, including RA, diabetes mellitus, and inflammatory bowel disease, and inoculation of periodontopathogenic bacteria (i.e. Pg) can alter gut microbiota composition. Therefore, this study investigated dysbiosis-mediated arthritis exacerbation by Pg oral inoculation in an experimental arthritis model mouse.Methods : Pg inoculation in the oral cavity twice a week for 6 weeks was performed to induce periodontitis in SKG mice. Concomitantly, a single intraperitoneal (i.p.) injection of laminarin (LA) was administered to induce experimental arthritis (Pg-LA mouse). Citrullinated protein (CP) and IL-6 levels in periodontal, intestinal, and joint tissues, and serum were measured by ELISA. Gut microbiota composition was determined by Miseq after DNA purification of mouse feces. Fecal microbiota transplantation (FMT) was performed by transferring Pg-RA-derived feces to normal mice. The effect of the Pg peptidylarginine deaminase (PgPAD) in arthritis progression was determined using PgPAD knockout mutant.Results : Periodontal alveolar bone loss and IL-6 in gingival tissue were induced by Pg oral infection, as well as severe joint destruction, increased arthritis scores (AS), and IL-6 and CP production in serum, joint, and intestinal tissues. Distribution of Deferribacteres and S24-7 was decreased, while CP was significantly increased in gingival, joint, and intestinal tissues of Pg-inoculated experimental arthritis mice compared to experimental arthritis mice without Pg inoculation. Further, FMT from Pg-inoculated experimental arthritis mice showed the reproduction of donor gut microbiota and resulted in severe joint destruction with increased IL-6 and CP production in joint and intestinal tissues. The maximum AS of FMT from Pg-inoculated experimental arthritis was much higher than that of donor mouse. However, inoculation of the PgPAD knockout mutant inhibited the elevation of arthritis scores, ACPA level in serum and reduced CP amount in gingival, joint, and intestinal tissues compared to Pg wild type inoculation .Conclusion : Pg oral infection exacerbated gut microbiota dysbiosis and joint destruction via increased CP generation.
Two splice variants of the DsMEK1 mitogen-activated protein kinase kinase (MAPKK) is involved different way in salt stress regulation in Dunaliella salina
Authors: Tang Ziyi , Cao Xiyue , Zhang Yiping , Jiang Jia , Qiao Dairong , Xu Hui , Cao Yi
Published: January 2020

Background：Dunaliella salina can produce a large amount of glycerol under salt stress, which can quickly adapt to the change of external salt concentration, and glycerol is one of the ideal energy sources. In recent years, it has been reported that Mitogen-activated protein kinase cascade pathway plays an important role in regulating salt stress, and in Dunaliella tertiolecta DtMAPK can regulate glycerol synthesis under salt stress. Therefore, it is urgent to study the relationship between MAPK cascade pathway and salt stress in D. salina, and help it to increase the content of glycerol. Results： In our study, we identified and analyzed the alternative splicing of DsMEK1 (DsMEK1-X1, DsMEK1-X2) from the unicellular green alga D. salina. DsMEK1-X1, DsMEK1-X2 both localized in the cytoplasm. The qRT-PCR assays showed that DsMEK1-X2 induced by salt stress. Overexpression of DsMEK1-X2 revealed a higher increase rate of glycerol compared to the control and DsMEK1-X1-oe under salt stress. The expression of DsGPDH2/3/5/6 increased in DsMEK1-X2-oe strains compared to the control under salt stress. It means that DsMEK1-X2 is involved in the regulation of DsGPDHs expression and glycerol overexpression under salt stress. Overexpression of DsMEK1-X1 increasing the proline content and reducing the MDA content under salt stress, and DsMEK1-X1 can regulate oxidative stress, thus we speculate that DsMEK1-X1 can reduce the damage of oxidative under salt stress. Yeast two-hybrid analysis showed that DsMEK1-X2 can interact with DsMAPKKK1/2/3/9/10/17 and DsMAPK1, however, DsMEK1-X1 interacted with neither upstream MAPKKK nor downstream MAPK. DsMEK1-X2-oe transgenic lines increased the expression of DsMAPKKK1/3/10/17 and DsMAPK1, and DsMEK1-X2-RNAi lines decreased the expression of DsMAPKKK2/10/17. DsMEK1-X1-oe transgenic lines do not increased genes expression, except for DsMAPKKK9. Conclusion： Our findings demonstrate that DsMEK1-X1 and DsMEK1-X2 can response to salt stress in two different pathways, DsMEK1-X1 response to salt stress by reducing oxidative damage, however, DsMAPKKK1/2/3/9/10/17- DsMEK1-X2-DsMAPK1 cascade is involved in the regulated of DsGPDH expression and thus glycerol synthesis under salt stress.
Improvement in functional gait parameters following corrective thoracolumbar surgery in children affected by Mucopolysaccharidosis 1(Hurler syndrome)
Authors: Sundarapandian Rajkumar , Jones Simon , Broomfield Alexander , Hensman Pauline , Oxborrow Neil
Published: January 2020

ObjectiveThoracolumbar kyphosis is a common indication for spinal surgery in children with Mucopolysaccharidosis. Functional outcome of spinal surgical intervention has never been published in patients with this rare disease. We present a cohort of patients with Mucopolysaccharidosis 1(Hurler syndrome) who underwent thoraco-lumbar spinal deformity correction and functional outcome assessed by pre-operative and post-operative gait analysis. This study represents the first attempt at presenting a functional assessment of surgical outcome in any Mucopolysaccharidosis subtype. MethodsA retrospective analysis of prospectively collected data was carried out from 11 children diagnosed with this subtype of Mucopolysaccharidosis. All patients underwent thoracolumbar kyphosis correction between the years 2013 to 2016. Gait assessment was performed using GAITRiteTM electronic walkway pre-operatively and post-operatively within 9 to 24 months from the index surgery. Walking distance, cadence and gait velocity were the three spatio-temporal parameters analysed. Wilcoxon signed rank test was used to analyse the data and P-Value £0.05 was deemed significant.ResultsThere was a statistically significant improvement in walking distance in 9 out of 11 patient post-operatively with a mean increase of 232.06 cms (P=0.05). There was marginal improvement in cadence by 6.33 steps/min post-operatively (P-value 0.79). Gait velocity also showed a marginal increase by 8.73 cms/sec post-operatively (P-value 0.32).ConclusionThe results of our study suggest that correction of thoracolumbar kyphosis in children with Mucopolysaccharidosis 1 resulted in a significant improvement of walking distance with a trend towards improved gait in the other parameters. Post-operative change in cadence was not statistically significant suggesting that physiological maturation of gait had minimal effect in the specified post-operative assessment timeframe. This study emphasizes that outcomes of spinal surgery in children with Mucopolysaccharidosis 1 should be determined by functional measures aiming to maintain or improve quality of life.
Efficacy of HIV interventions among factory workers in low- and middle-income countries: a systematic review
Authors: Chen Dahui , Luo Ganfeng , Wang Zixin , Cai Yong , Meng Xiaojun , Yuan Tanwei , Xie Yu , Hu Tian , Wang Zhenyu , Zhang Kechun , Zou Huachun
Published: January 2020

Background Factory workers in low- and middle-income countries (LMICs) are vulnerable to HIV transmission. Interventions are needed to prevent HIV in this population. We systematically reviewed published literature on the efficacy of various HIV interventions in reducing stigma, risk behaviors and HIV transmission among factory workers.Methods A systematic review was performed using predefined inclusion and exclusion criteria. Four databases (PubMed, PsycINFO, Scopus and EMBASE) were searched for relevant publications between January 1, 1990 and December 31, 2018. Two independent reviewers assessed the methodological quality of studies.Results Thirteen articles were included, with 2 randomized controlled trials and 11 cohort studies. Five interventions and their combinations were summarized. Educational intervention increased condom use and reduced the use of recreational drugs and alcohol before sex. Community intervention indicated that workers at high risk behaviors were more likely to go to HIV counselling and testing (HCT), and proactive provision of HCT could increase the detection rate of HIV and sexually transmitted diseases (STDs). Lottery intervention increased HCT uptake and decreased HIV public stigma. Education combined with community intervention reduced the proportion of workers with casual sex and enhanced HIV knowledge. Peer education combined with community intervention increased the proportion of workers who were willing to take their partners to HCT. Policy intervention combined with peer education enhanced HIV knowledge, perceived condom accessibility and condom use with regular partners.Conclusions Various interventions improved HIV knowledge, decreased HIV stigma and reduced HIV-related risk behaviors among factory workers in LMICs. The combination of multiple interventions tended to achieve better efficacy than a single intervention. Persistent combination interventions are essential to address HIV in this population.
Development and Evaluation of a Physical Activity Intervention Informed by Participatory Research- A Feasibility Study
Authors: Ramji Rathi , Carlson Elisabeth , Kottorp Anders , Shleev Sergey , Awad Eman , Rämgård Margareta
Published: January 2020

Background: Despite numerous interventions aiming to improve physical activity in socially disadvantaged populations, physical inactivity remains to be a rising challenge to public health globally, as well as, in Sweden. In an effort to address this challenge, a community-based participatory intervention was developed through active community engagement and implemented in a socially disadvantaged neighborhood in Sweden. The current study aims to present the development and initial evaluation of a participatory research driven physical activity intervention. Methods : Fifteen participants (11 females and 4 males) aged 17-59 years volunteered to participate in the physical activity intervention program. The intervention program was evaluated using a longitudinal mixed methods design measuring health impact changes over time through focus group discussions and quality of life surveys. Further additional biomedical health parameters such as levels of glycosylated hemoglobin, blood pressure, levels of oxygen saturation and body mass index were monitored before and after the intervention. Focus group data were analyzed using content analysis with an inductive approach. The pre-and post-test scores from the survey-based quality of life domains, as well as the health parameters were compared using non-parametric and parametric statistics. Results : Four themes emerged from the analysis of the focusgroup discussions including sense of fellowship, striving for inclusion and equity, changing the learner perspective and health beyond illness. The scores for the domains Physical Health, Psychological Health, Social Relationships and Health Satisfaction where significantly higher after participation in the physical activity intervention program compared to the pre-test scores ( p <.05)s. There were however, no significant changes in the scores for the environmental domain and overall quality of life after intervention compared to that prior to intervention start. Overall, the biomedical health parameters remained stable within the normal ranges during intervention. Conclusion : The focusgroup discussions and results from the surveys and biomedical measures reveal important findings to understand and further develop the intervention program to promote health equity among citizens in disadvantaged areas. Evaluating the feasibility of such an intervention using multiple approaches contributes to effective implementation of it for larger communities in need.
External validation of simplified out-of-hospital cardiac arrest and cardiac arrest hospital prognosis scores in a Japanese population: A multicentre retrospective cohort study
Authors: Shibahashi Keita , Sugiyama Kazuhiro , Kuwahara Yusuke , Ishida Takuto , Sakurai Atsushi , Kitamura Nobuya , Tagami Takashi , Nakada Taka-aki , Takeda Munekazu , Hamabe Yuichi
Published: January 2020

Background Out-of-hospital cardiac arrest (OHCA) is a global medical problem. The newly-developed simplified out-of-hospital cardiac arrest (sOHCA) and cardiac arrest hospital prognosis (sCAHP) scores used for prognostication of patients admitted alive have not been validated externally. This study was, thus, conducted to externally validate sOHCA and sCAHP scores in a Japanese population. Methods Adult patients resuscitated and admitted to hospitals after intrinsic OHCA (n=2,428, age ≥18 years) were selected from a prospectively collected Japanese database (January 2012–March 2013). We validated sOHCA and sCAHP scores with reference to the original ones in predicting 1-month unfavourable neurological outcomes based on discrimination and calibration measures. Discrimination and calibration were assessed using area under the receiver operating characteristic curve (AUC) and the Hosmer-Lemeshow goodness-of-fit test with calibration plot, respectively. Results One-month unfavourable neurological outcome was observed in 82% of patients. Score availability was significantly higher in the simplified scores than in the original ones and was highest in the sCAHP score (76%). The AUCs of simplified scores were not significantly different from those of original ones, whereas the AUC of the sCAHP score was significantly higher than that of the sOHCA score (0.88 vs. 0.81, P <0.001). Goodness-of-fit was poor in the sOHCA score (ν= 8, χ 2 =19.1, Hosmer-Lemeshow test: P =0.014) but not in the sCAHP score (ν= 8, χ 2 =13.5, Hosmer-Lemeshow test: P =0.10). Conclusion Performance of original and simplified OHCA and CAHP scores in predicting neurological outcomes in successfully resuscitated OHCA patients were acceptable. Based on the highest availability, similar discrimination, and good calibration, the sCAHP score was the better candidate for clinical implementation. The validated predictive score can help patients’ families, healthcare providers, and researchers by accurately stratifying patients.
Predicting the occurrence of venous thromboembolism: construction and verification of Risk Warning Model
Authors: Shen Chen , Ge Binqian , Liu Xiaoqin , Chen Hao , Qin Yi , Shen Hongwu
Published: January 2020

Background: The onset of venous thromboembolism is insidious and the prognosis is poor. In this study, we aimed to construct a VTE risk early warning model and explore the clinical application value of the VTE risk early warning model. Methods: Preliminary construction of the VTE risk warning model was carried out according to the independent risk warning indicators of VTE screened by Logistic regression analysis in previous studies. The truncated value of screening VTE was obtained and the model was evaluated. ROC curve analysis was used to compare the test performance of Caprini risk assessment scale and VTE risk warning model on VTE. The validation data set was established, and the cut-off value of the VTE risk warning model was used to evaluate the test effectiveness of the model for VTE patients with validation data set. Results: The VTE risk warning model is p = ex / ( 1+ ex ) , x = -4.840 + 2.557 • X10(1) + 1.432 • X14(1) + 2.977 • X15(1) + 3.445 • X18(1) + 1.086 • X25(1) + 0.249 • X34 + 0.282 • X41. ROC curve results show that: AUC (95%CI), cutoff value (95%CI), accuracy, Youden index (95%), sensitivity, specificity and other evaluation indexes, Caprini risk assessment scale is 0.596 (0.552, 0.638), > 5 (> 4, > 5), 61.3%, 0.226 (0.167, 0.290), 26.07%, 96.50%, VTE risk warning model is 0.960 (0.940, 0.976), > 0.438 (> 0.263), respectively. >, 0.504), 92.2%, 0.844 (0.789, 0.879), 92.61%, 91.83%, with statistically significant differences (Z=14.521, P < 0.0001). The accuracy and Youden index of VTE screening using VTE risk warning model were 81.8% and 62.5%, respectively. Conclusions: VTE risk warning model has high accuracy in predicting the occurrence of VTE in hospitalized patients, and its test performance is higher than Caprini risk assessment scale. It also has high test performance for VTE in external population.
MicroRNA-491-5p inhibit gastric cancer development by targeting EMT, cell adhesion genes and IFITM2
Authors: Wei Zhijian , Zhang Lixiang , Li Angqing , Li Chuanhong , Han Wenxiu , Xu A-Man
Published: January 2020

Gastric cancer (GC) is one of the deadliest cancers in China. And, it can be regulated by MicroRNAs (miRNAs) generally. miR-491-5p function as a tumor suppressor in different types of cancer, but we still don’t know the role of miR-491-5p in gastric cancer. In this study, we found that high level of miR-491-5p caused a weak cell proliferation, migration and invasion abilities. In order to explore the role of miR-491-5p in vivo, we set a xenograft mouse model, and also found that high level of miR-491-5p suppressed tumor growth. Moreover, we found that miR-491-5p regulate the tumor development thought regulate the expression of EMT, cell adhesion genes and IFITM2. These data show that miR-491-5p function as a tumor suppressor in GC both in vitro and in vivo .
Synthesis and Preclinical Evaluation of 68Ga-MALAT-1 ASO for PET Imaging of MALAT-1 expressing Tumours
Authors: Liu Zhen-Feng , Yang Jun , Ye Qianni , Yang Min , Pan Dong-hui , Xu Yu-ping , Dong Mengjie
Published: January 2020

Background: MALAT-1 (Metastasis-Associated Lung Adenocarcinoma Transcript 1) is a large long nuclear noncoding RNA (lncRNA) that is overexpressed in an array of cancers. In this study, we designed a range of positron probes for MALAT-1 to evaluate its distribution, pharmacokinetics, and to explore whether the probe can be used for the imaging of malignant tumors with high MALAT-1 expression in vivo. Methods: 68Ga labelling of MALAT-1 antisense oligonucleotides (68Ga–MALAT-1 ASO) was synthesized by the conjugation of MALAT-1 NOTA-ASO and 68Ga3+. Purity was assessed by radio-HPLC. Pharmacokinetic studies and cell uptake were assessed. The biodistribution and metabolism of 68Ga–MALAT-1 ASO in normal ICR and MHCC-LM3 xenograft-bearing nude mice were studied. Results: 68Ga–MALAT-1 ASO was obtained at a radiochemical yield of 98% from a 10 min synthesis with 100 ± 50 MBq/nmol activity and > 99% purity once synthesized. The Log P was -2.53±0.19. The tracer displayed excellent stability in vitro. 68Ga–MALAT-1 ASO showed satisfactory binding ability to MHCC-LM3 cells; the biodistribution of 68Ga-MALAT-1 ASO in MHCC-LM3 tumour-bearing mice showed high levels of uptake (3.04 ± 0.11%ID/g). Micro-PET scans demonstrated the tumor specific uptake of 68Ga-MALAT-1 ASO in mouse models. Conclusions: We conclude that 68Ga labelling of MALAT-1 ASO is a convenient approach to label tumors overexpressing MALAT-1.
Risk factors of the Secondary Fractures for Osteoporotic Vertebral Compression Fractures after Percutaneous Vertebroplasty
Authors: Kang Xiao-kui , Guo Sheng-fu , Liu Hui-xin , Huang Li-li , Jiang Qun-long
Published: January 2020

Background Percutaneous vertebroplasty related postoperative secondary fractures risk factors were not consistent in patients with osteoporotic vertebral compression Fractures. The purpose was to identify the risk factors of the secondary fractures for osteoporotic vertebral compression fractures after percutaneous vertebroplasty.Methods Potential academic articles were identified from Cochrane Library, Medline, PubMed, Embase, ScienceDirect and other databases. The time range we retrieved from was that from the inception of electronic databases to August 2019. Gray studies were identified from the references of included literature reports. STATA version 11.0 (Stata Corporation, College Station, Texas, USA) was used to analyze the pooled data.Results Fourteen studies involving 1910 patients, 395 of whom had secondary fracture following the surgery were included in this meta-analysis. The results of meta-analysis showed the risk factors of the secondary fractures for osteoporotic vertebral compression fractures after percutaneous vertebroplasty was related to bone mineral density [WMD= -0.518, 95%CI(-0.784,-0.252), P=0.000], cement leakage [RR=0.596, 95%CI (0.444,0.798), P=0.001] and kyphosis after primary operation [WMD=4.510, 95%CI (3.061,6.004),P=0.000], but not to gender, age, body mass index (BMI), cement volume, thoracolumbar spine, and cement injection approaches.Conclusions BMD, cement leakage and kyphosis after primary operation are the risk factors closely correlative to the secondary fracture after percutaneous vertebroplasty. There has not been enough evidence to support the association between the secondary fracture and gender, age, body mass index, cement volume, thoracolumbar spine, and cement injection approach.