bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical practice guidelines for the diagnosis and surveillance of BAP1 tumour predisposition syndrome
Authors: Lalloo Fiona , Kulkarni Anju , Chau Cindy , Nielsen Maartje , Sheaff Michael , Steele Jeremy , van Doorn Remco , Wadt Karin , Hamill Monica , Torr Beth , Tischkowitz Marc ,   Delphi respondents, Ahmed Munaza , Bajalica-Lagercrantz Svetlana , Blatnik Ana , Brunet Joan , Cleaver Ruth , Colas Chrystelle , Dabir Tabib , Evans D. Gareth , Feshtali Shirin , Ghiorzo Paola , Graversen Lise , Griewank Klaus , Helgadottir Hildur , Jewell Rosalyn , Kohut Kelly , Lorentzen Henrik , Massi Daniela , Missotten Guy , Murray Alex , Murray Jennie , Nadal Ernest , Ong Kai Ren , Piulats Josep M. , Puig Susana , Rajan Neil , Ribero Simone , Salle Galateau , Teulé Alexandre , Tham Emma , van Paassen Barbara , De Putter Robin , Verdijk Robert , Wagner Anja , Woodward Emma R. , Hanson Helen
Published: August 2023

BRCA1-associated protein-1 (BAP1) is a recognised tumour suppressor gene. Germline BAP1 pathogenic/likely pathogenic variants are associated with predisposition to multiple tumours, including uveal melanoma, malignant pleural and peritoneal mesothelioma, renal cell carcinoma and specific non-malignant neoplasms of the skin, as part of the autosomal dominant BAP1-tumour predisposition syndrome. The overall lifetime risk for BAP1 carriers to develop at least one BAP1-associated tumour is up to 85%, although due to ascertainment bias, current estimates of risk are likely to be overestimated. As for many rare cancer predisposition syndromes, there is limited scientific evidence to support the utility of surveillance and, therefore, management recommendations for BAP1 carriers are based on expert opinion. To date, European recommendations for BAP1 carriers have not been published but are necessary due to the emerging phenotype of this recently described syndrome and increased identification of BAP1 carriers via large gene panels or tumour sequencing. To address this, the Clinical Guideline Working Group of the CanGene-CanVar project in the United Kingdom invited European collaborators to collaborate to develop guidelines to harmonize surveillance programmes within Europe. Recommendations with respect to BAP1 testing and surveillance were achieved following literature review and Delphi survey completed by a core group and an extended expert group of 34 European specialists including Geneticists, Ophthalmologists, Oncologists, Dermatologists and Pathologists. It is recognised that these largely evidence-based but pragmatic recommendations will evolve over time as further data from research collaborations informs the phenotypic spectrum and surveillance outcomes.
Combining Fe nanoparticles and pyrrole-type Fe-N4 sites on less-oxygenated carbon supports for electrochemical CO2 reduction
Authors: Wang Cai , Wang Xiaoyu , Ren Houan , Zhang Yilin , Zhou Xiaomei , Wang Jing , Guan Qingxin , Liu Yuping , Li Wei
Published: August 2023

A great challenge for electrochemical CO2 reduction is to improve energy efficiency, which requires reducing overpotential while increasing product Faraday efficiency. Here, we designedly synthesize a hybrid electrocatalyst consisting of Fe nanoparticles, pyrrole-type Fe-N4 sites and less-oxygenated carbon supports, which exhibits a remarkable CO Faraday efficiency above 99% at an ultralow overpotential of 21 mV, reaching the highest cathode energy efficiency of 97.1% to date. The catalyst also can afford a CO selectivity nearly 100% with a high cathode energy efficiency (>90%) at least 100 h. The combined results of control experiments, in situ characterizations and theoretical calculations demonstrate that introducing Fe nanoparticles can reduce the overpotential by accelerating the proton transfer from CO2 to *COOH and lowering the free energy for *COOH formation, constructing pyrrole-type Fe-N4 sites and limiting oxygen species on carbon supports can increase CO Faraday efficiency through inhibiting the H2 evolution, thus achieving energy-efficient electrochemical CO2 reduction to CO.
Molecular features and clinical implications of the heterogeneity in Chinese patients with HER2-low breast cancer
Authors: Dai Lei-Jie , Ma Ding , Xu Yu-Zheng , Li Ming , Li Yu-Wei , Xiao Yi , Jin Xi , Wu Song-Yang , Zhao Ya-Xin , Wang Han , Yang Wen-Tao , Jiang Yi-Zhou , Shao Zhi-Ming
Published: August 2023

The molecular heterogeneity and distinct features of HER2-low breast cancers, particularly in the Chinese population, are not well understood, limiting its precise management in the era of antibody‒drug conjugates. To address this issue, we established a cohort of 434 Chinese patients with HER2-low breast cancer (433 female and one male) and integrated genomic, transcriptomic, proteomic, and metabolomic profiling data. In this cohort, HER2-low tumors are more distinguished from HER2-0 tumors in the hormone receptor–negative subgroup. Within HER2-low tumors, significant interpatient heterogeneity also exists in the hormone receptor–negative subgroup: basal-like tumors resemble HER2-0 disease, and non-basal-like HER2-low tumors mimic HER2-positive disease. These non-basal-like HER2-low tumors are enriched in the HER2-enriched subtype and the luminal androgen receptor subtype and feature PIK3CA mutation, FGFR4/PTK6/ERBB4 overexpression and lipid metabolism activation. Among hormone receptor–positive tumors, HER2-low tumors show less loss/deletion in 17q peaks than HER2-0 tumors. In this work, we reveal the heterogeneity of HER2-low breast cancers and emphasize the need for more precise stratification regarding hormone receptor status and molecular subtype.
Cultivation of marine bacteria of the SAR202 clade
Authors: Lim Yeonjung , Seo Ji-Hui , Giovannoni Stephen J. , Kang Ilnam , Cho Jang-Cheon
Published: August 2023

Bacteria of the SAR202 clade, within the phylum Chloroflexota, are ubiquitously distributed in the ocean but have not yet been cultivated in the lab. It has been proposed that ancient expansions of catabolic enzyme paralogs broadened the spectrum of organic compounds that SAR202 bacteria could oxidize, leading to transformations of the Earth’s carbon cycle. Here, we report the successful cultivation of SAR202 bacteria from surface seawater using dilution-to-extinction culturing. The growth of these strains is very slow (0.18–0.24 day−1) and is inhibited by exposure to light. The genomes, of ca. 3.08 Mbp, encode archaella (archaeal motility structures) and multiple sets of enzyme paralogs, including 80 genes coding for enolase superfamily enzymes and 44 genes encoding NAD(P)-dependent dehydrogenases. We propose that these enzyme paralogs participate in multiple parallel pathways for non-phosphorylative catabolism of sugars and sugar acids. Indeed, we demonstrate that SAR202 strains can utilize several substrates that are metabolized through the predicted pathways, such as sugars ʟ-fucose and ʟ-rhamnose, as well as their lactone and acid forms.
Pyruvate dehydrogenase operates as an intramolecular nitroxyl generator during macrophage metabolic reprogramming
Authors: Palmieri Erika M. , Holewinski Ronald , McGinity Christopher L. , Pierri Ciro L. , Maio Nunziata , Weiss Jonathan M. , Tragni Vincenzo , Miranda Katrina M. , Rouault Tracey A. , Andresson Thorkell , Wink David A. , McVicar Daniel W.
Published: August 2023

M1 macrophages enter a glycolytic state when endogenous nitric oxide (NO) reprograms mitochondrial metabolism by limiting aconitase 2 and pyruvate dehydrogenase (PDH) activity. Here, we provide evidence that NO targets the PDH complex by using lipoate to generate nitroxyl (HNO). PDH E2-associated lipoate is modified in NO-rich macrophages while the PDH E3 enzyme, also known as dihydrolipoamide dehydrogenase (DLD), is irreversibly inhibited. Mechanistically, we show that lipoate facilitates NO-mediated production of HNO, which interacts with thiols forming irreversible modifications including sulfinamide. In addition, we reveal a macrophage signature of proteins with reduction-resistant modifications, including in DLD, and identify potential HNO targets. Consistently, DLD enzyme is modified in an HNO-dependent manner at Cys477 and Cys484, and molecular modeling and mutagenesis show these modifications impair the formation of DLD homodimers. In conclusion, our work demonstrates that HNO is produced physiologically. Moreover, the production of HNO is dependent on the lipoate-rich PDH complex facilitating irreversible modifications that are critical to NO-dependent metabolic rewiring.
Desmoplastic stroma restricts T cell extravasation and mediates immune exclusion and immunosuppression in solid tumors
Authors: Xiao Zebin , Todd Leslie , Huang Li , Noguera-Ortega Estela , Lu Zhen , Huang Lili , Kopp Meghan , Li Yue , Pattada Nimisha , Zhong Wenqun , Guo Wei , Scholler John , Liousia Maria , Assenmacher Charles-Antoine , June Carl H. , Albelda Steven M. , Puré Ellen
Published: August 2023

The desmoplastic stroma in solid tumors presents a formidable challenge to immunotherapies that rely on endogenous or adoptively transferred T cells, however, the mechanisms are poorly understood. To define mechanisms involved, here we treat established desmoplastic pancreatic tumors with CAR T cells directed to fibroblast activation protein (FAP), an enzyme highly overexpressed on a subset of cancer-associated fibroblasts (CAFs). Depletion of FAP+ CAFs results in loss of the structural integrity of desmoplastic matrix. This renders these highly treatment-resistant cancers susceptible to subsequent treatment with a tumor antigen (mesothelin)-targeted CAR T cells and to anti-PD-1 antibody therapy. Mechanisms include overcoming stroma-dependent restriction of T cell extravasation and/or perivascular invasion, reversing immune exclusion, relieving T cell suppression, and altering the immune landscape by reducing myeloid cell accumulation and increasing endogenous CD8+ T cell and NK cell infiltration. These data provide strong rationale for combining tumor stroma- and malignant cell-targeted therapies to be tested in clinical trials.
Aqueous pulsed electrochemistry promotes C−N bond formation via a one-pot cascade approach
Authors: He Meng , Wu Yongmeng , Li Rui , Wang Yuting , Liu Cuibo , Zhang Bin
Published: August 2023

Electrocatalytic C − N bond formation from inorganic nitrogen wastes is an emerging sustainable method for synthesizing organic amines but is limited in reaction scope. Integrating heterogeneous and homogeneous catalysis for one-pot reactions to construct C − N bonds is highly desirable. Herein, we report an aqueous pulsed electrochemistry-mediated transformation of nitrite and arylboronic acids to arylamines with high yields. The overall process involves nitrite electroreduction to ammonia over a Cu nanocoral cathode and subsequent coupling of NH3 with arylboronic acids catalyzed by in situ dissolved Cu(II) under a switched anodic potential. This pulsed protocol also promotes the migration of nucleophilic ArB(OH)3− and causes the consumption of OH− near the cathode surface, accelerating C − N formation and suppressing phenol byproducts. Cu(II) can be recycled via facile electroplating. The wide substrate scope, ready synthesis of 15N-labelled arylamines, and methodological expansion to cycloaddition and Click reactions highlight the great promise.
Glycome profiling and immunohistochemistry uncover changes in cell walls of Arabidopsis thaliana roots during spaceflight
Authors: Nakashima Jin , Pattathil Sivakumar , Avci Utku , Chin Sabrina , Alan Sparks J. , Hahn Michael G. , Gilroy Simon , Blancaflor Elison B.
Published: August 2023

A large and diverse library of glycan-directed monoclonal antibodies (mAbs) was used to determine if plant cell walls are modified by low-gravity conditions encountered during spaceflight. This method called glycome profiling (glycomics) revealed global differences in non-cellulosic cell wall epitopes inArabidopsis thalianaroot extracts recovered from RNA purification columns between seedlings grown on the International Space Station-based Vegetable Production System and paired ground (1-g) controls. Immunohistochemistry on 11-day-old seedling primary root sections showed that ten of twenty-two mAbs that exhibited spaceflight-induced increases in binding through glycomics, labeled space-grown roots more intensely than those from the ground. The ten mAbs recognized xyloglucan, xylan, and arabinogalactan epitopes. Notably, three xylem-enriched unsubstituted xylan backbone epitopes were more intensely labeled in space-grown roots than in ground-grown roots, suggesting that the spaceflight environment accelerated root secondary cell wall formation. This study highlights the feasibility of glycomics for high-throughput evaluation of cell wall glycans using only root high alkaline extracts from RNA purification columns, and subsequent validation of these results by immunohistochemistry. This approach will benefit plant space biological studies because it extends the analyses possible from the limited amounts of samples returned from spaceflight and help uncover microgravity-induced tissue-specific changes in plant cell walls.
A large public dataset of annotated clinical MRIs and metadata of patients with acute stroke
Authors: Liu Chin-Fu , Leigh Richard , Johnson Brenda , Urrutia Victor , Hsu Johnny , Xu Xin , Li Xin , Mori Susumu , Hillis Argye E. , Faria Andreia V.
Published: August 2023

To extract meaningful and reproducible models of brain function from stroke images, for both clinical and research proposes, is a daunting task severely hindered by the great variability of lesion frequency and patterns. Large datasets are therefore imperative, as well as fully automated image post-processing tools to analyze them. The development of such tools, particularly with artificial intelligence, is highly dependent on the availability of large datasets to model training and testing. We present a public dataset of 2,888 multimodal clinical MRIs of patients with acute and early subacute stroke, with manual lesion segmentation, and metadata. The dataset provides high quality, large scale, human-supervised knowledge to feed artificial intelligence models and enable further development of tools to automate several tasks that currently rely on human labor, such as lesion segmentation, labeling, calculation of disease-relevant scores, and lesion-based studies relating function to frequency lesion maps.
The proteome of extracellular vesicles of the lung fluke Paragonimus kellicotti produced in vitro and in the lung cyst
Authors: Di Maggio Lucia S. , Fischer Kerstin , Yates Devyn , Curtis Kurt C. , Rosa Bruce A. , Martin John , Erdmann-Gilmore Petra , Sprung Robert S. W. , Mitreva Makedonka , Townsend R. Reid , Weil Gary J. , Fischer Peter U.
Published: August 2023

Paragonimiasis is a zoonotic, food-borne trematode infection that affects 21 million people globally. Trematodes interact with their hosts via extracellular vesicles (EV) that carry protein and RNA cargo. We analyzed EV in excretory-secretory products (ESP) released by Paragonimus kellicotti adult worms cultured in vitro (EV ESP) and EV isolated from lung cyst fluid (EV CFP) recovered from infected gerbils. The majority of EV were approximately 30–50 nm in diameter. We identified 548 P. kellicotti-derived proteins in EV ESP by mass spectrometry and 8 proteins in EV CFP of which 7 were also present in EV ESP. No parasite-derived proteins were reliably detected in EV isolated from plasma samples. A cysteine protease (MK050848, CP-6) was the most abundant protein found in EV CFP in all technical and biological replicates. Immunolocalization of CP-6 showed strong labeling in the tegument of P. kellicotti and in the adjacent cyst and lung tissue that contained worm eggs. It is likely that CP-6 present in EV is involved in parasite-host interactions. These results provide new insights into interactions between Paragonimus and their mammalian hosts, and they provide potential clues for development of novel diagnostic tools and treatments.
Probing densified silica glass structure by molecular oxygen and E’ center formation under electron irradiation
Authors: Ollier N. , Reghioua I. , Cavani O. , Mobasher M. , Alessi A. , le Floch S. , Skuja L.
Published: August 2023

This study aims to learn more about the structure of densified silica with focus on the metamict-like silica phase (density = 2.26 g/cm3) by examining the formation of E’ point defects and interstitial molecular oxygen O2 by 2.5 MeV electron irradiation. High-dose (11 GGy) irradiation creates a metamict-like phase and a large amount of interstitial O2, which is destroyed upon subsequent additional lower-dose electron irradiation. The O2 cathodoluminescence (CL) data indicate that the formation of O2 from peroxy linkages Si–O–O–Si in silica network is strongly dependent on the intertetrahedral void sizes. The position and shape of the O2 emission line support the idea that the configuration of these voids in metamict phase is close to that of non-densified silica. Moreover, data support the strong correlation between the formation of 3-membered rings of Si–O bonds and E’-centers when silica density increases from 2.20 to 2.26 g/cm3.
Circadian gene variation in relation to breeding season and latitude in allochronic populations of two pelagic seabird species complexes
Authors: Birchard Katie , Driver Hannah G. , Ademidun Dami , Bedolla-Guzmán Yuliana , Birt Tim , Chown Erin E. , Deane Petra , Harkness Bronwyn A. S. , Morrin Austin , Masello Juan F. , Taylor Rebecca S. , Friesen Vicki L.
Published: August 2023

Annual cues in the environment result in physiological changes that allow organisms to time reproduction during periods of optimal resource availability. Understanding how circadian rhythm genes sense these environmental cues and stimulate the appropriate physiological changes in response is important for determining the adaptability of species, especially in the advent of changing climate. A first step involves characterizing the environmental correlates of natural variation in these genes. Band-rumped and Leach’s storm-petrels (Hydrobates spp.) are pelagic seabirds that breed across a wide range of latitudes. Importantly, some populations have undergone allochronic divergence, in which sympatric populations use the same breeding sites at different times of year. We investigated the relationship between variation in key functional regions of four genes that play an integral role in the cellular clock mechanism—Clock, Bmal1, Cry2 and Per2—with both breeding season and absolute latitude in these two species complexes. We discovered that allele frequencies in two genes, Clock and Bmal1, differed between seasonal populations in one archipelago, and also correlated with absolute latitude of breeding colonies. These results indicate that variation in these circadian rhythm genes may be involved in allochronic speciation, as well as adaptation to photoperiod at breeding locations.
Use of the Neuroform Atlas stent for wide-necked cerebral aneurysms
Authors: Gao Hui-Li , Shao Qiu-Ji , Chang Kai-Tao , Li Li , Li Tian-Xiao , Gao Bulang
Published: August 2023

To investigate the effect and safety of the Neuroform Atlas (NFA) stent in stent-assisted coiling of wide-necked intracranial aneurysms, patients with wide-necked intracranial aneurysms were retrospectively enrolled and treated with the NFA stent-assisted coiling. The modified Rankin scale (mRS) grades and Raymond grades were used to assess the clinical outcomes and aneurysm occlusion degrees, respectively, after embolization and at follow-up. Totally, 122 patients were enrolled with 129 wide-necked aneurysms, and forty-nine (40.2%) patients experienced subarachnoid hemorrhage. A total of 134 NFA stents were deployed in all patients. Immediately after endovascular embolization, the Raymond grade was I in 112 (86.8%), II in 8 (6.2%), and III in 9 (7.0%). Complications occurred in 7 (5.7%) patients, including stent displacement in 2 (1.6%) patients, thrombosis and cerebral infarction in 4 (3.3%), and death in 1 (0.8%). Clinical follow-up was performed in 113 (92.6%) patients 6–30 (mean 21) months after embolization, with the mRS grade 0 in 99 (87.6%) patients, 1 in 7 (6.2%), 2 in 5 (4.4%), and 3 in 2 (1.8%). Good prognosis (mRS ≤ 2) was achieved in 111 (98.2%) patients while poor prognosis (mRS > 2) in two (1.8%). Digital subtraction angiography was conducted in 98 (80.3%) patients with 104 (80.6%) aneurysms 6–30 (mean 21) months after embolization. The Raymond grade was grade I in 94 (90.4%) aneurysms, II in 4 (3.8%), and III in 6 (5.8%). Compared with the Raymond grades immediately after embolization, 93 (89.4%) aneurysms disappeared, 9 (8.7%) remained unchanged in the occlusion status, and 2 (1.9%) were recurrent. In conclusion, the NFA stent may have a high aneurysm occlusion rate and a low complication rate in assisting coiling of wide-necked intracranial aneurysms even though further studies are necessary to prove this.
Quantitative estimation of phospholipid molecules desorbed from a microbubble surface under ultrasound irradiation
Authors: Kobayashi Reina , Narita Jun , Nakaoka Natsumi , Krafft Marie Pierre , Koyama Daisuke
Published: August 2023

Microbubbles have potential applications as drug and gene carriers, and drug release can be triggered by externally applied ultrasound irradiation while inside blood vessels. Desorption of molecules forming the microbubble shell can be observed under ultrasound irradiation of a single isolated microbubble, and the volume of desorbed molecules can be quantitatively estimated from the contact angle between the bubble and a glass plate. Microbubbles composed of a 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) shell and a poorly-soluble gas are created. When the microbubbles are exposed to a pulsed ultrasound, the contact angles increase dramatically; the percentage of DMPC molecules desorbed from the bubble surface reaches 70%. Vibration of a single bubble in the radial direction is measured using a laser Doppler vibrometer. The relationship between the vibrational characteristics and the amount of molecular desorption reveals that a larger vibrational amplitude of the bubble around the resonance size induces a larger amount of molecular desorption. These results support the possibility of controlling molecular desorption with pulsed ultrasound.
Stability, optimum ultrasonication, and thermal and electrical conductivity estimation in low concentrations of Al12Mg17 nanofluid by dynamic light scattering and beam displacement method
Authors: Javadipour Soroush , Shokuhfar Ali , Heidary Zeinab , Amiri Roshkhar Mohammad Amin , Homayouni Keyvan , Rezaei Fatemeh , Zolriasatein Ashkan , Shahhosseini Shahrokh , Rashidi Alimorad , Khamoushi S. M. Mahdi
Published: August 2023

The thermal conductivity and stability of nanofluids pose challenges for their use as coolants in thermal applications. The present study investigates the heat transfer coefficient (HTC) of an Al12Mg17 nanofluid through the utilization of a novel beam displacement method. The study also examines the nanofluid's stability, particle size distribution (PSD), TEM micrograph, and electrical conductivity. From three distinct categories of surfactants, a particular surfactant (CTAB) was chosen to disperse Al12Mg17 nanoparticles in DI water, and subsequently, a two-step method was employed to generate the nanofluid. Dispersion stability is visually monitored and quantified with a zeta potential test. HTC and PSD are measured using optical setups. To evaluate the results, the HTC obtained from the beam displacement method is compared with that of the KD2 Pro apparatus, and the PSD findings are analyzed through TEM micrographs. The results show that a 0.16 vol.% CTAB is the maximum stability for 0.025 vol.% Al12Mg17 nanofluid properly. The optimum ultrasonication period is 2 h, yielding a peak PSD of 154 nm. Increasing nanoparticle concentration enhances HTC up to 40% compared to the base fluid at 0.05 vol.%. Electrical conductivity increases linearly from 155 to 188 μ$${\rm S}/\mathrm{cm}$$

S
/
cm

with nanoparticle concentration. Optical methods for measuring HTC in nanofluids offer the advantage of early results, prior to bulk motion. Thus, the application of nanofluids in thermal systems necessitates the development of optical techniques to improve accuracy.
Factors affecting insulation failure in reusable surgical devices
Authors: Homma Takahiro , Uehara Hirofumi , Saji Hisashi
Published: August 2023

The purpose of this study was to investigate the associated factors of insulation failure (IF) in reusable endoscopic instruments. The insulation coating of reusable endoscopic instruments underwent routine visual checks, hand washing to remove visible stains, and mechanized sterilization. We recorded the cleaning number and usage period of all instruments. The instruments were tested for IF using a detector. IF was found in eight of 69 devices (11.6%). Examining by clinical specialty, we found IF in 4 of 28 gastrointestinal (14.3%), 3 of 20 gynecological (15.0%), 1 of 12 urological (8.3%), and none of the nine thoracic devices. The median distance from the tip to the damaged part was 5 cm (3–5 cm). In the IF and the intact groups, the period of use [7 years (6–8) versus 7 years (4–8), P = 0.90] and the number of cleanings [281 (261–323) versus 261 (179–320), P = 0.27] were not significantly different. The IF group included products of three different companies; however, six of the eight (75.0%) were from the same company. Cleaning methods and usage period have a lower impact on IF. The use of reusable forceps as a monopolar device was found to pose a higher risk, requiring regular assessments.
Induction of open-form bile canaliculus formation by hepatocytes for evaluation of biliary drug excretion
Authors: Arakawa Hiroshi , Nakazono Yuya , Matsuoka Natsumi , Hayashi Momoka , Shirasaka Yoshiyuki , Hirao Atsushi , Tamai Ikumi
Published: August 2023

Biliary excretion is a major drug elimination pathway that affects their efficacy and safety. The currently available in vitro sandwich-cultured hepatocyte method is cumbersome because drugs accumulate in the closed bile canalicular lumen formed between hepatocytes and their amounts cannot be mealsured directly. This study proposes a hepatocyte culture model for the rapid evaluation of drug biliary excretion using permeation assays. When hepatocytes are cultured on a permeable support coated with the cell adhesion protein claudins, an open-form bile canalicular lumen is formed at the surface of the permeable support. Upon application to the basolateral (blood) side, drugs appear on the bile canalicular side. The biliary excretion clearance of several drugs, as estimated from the obtained permeabilities, correlates well with the reported in vivo biliary excretion clearance in humans. Thus, the established model is useful for applications in the efficient evaluation of biliary excretion during drug discovery and development.
Large dynamic range dual-mode pH sensors via dye-doped ionic liquid fiber optofluidic lasers
Authors: Li Zhi , Li Dongyang , Zhang Mengda , Jin Yingxia , Xu Qingbo , Yang Haiyan , Sun Yuze , Pu Xiaoyun , Li Liang , Zhang Yuanxian
Published: August 2023

Fiber optofluidic lasers using an RhB-doped ionic liquid (BmimPF6) as the gain medium can realize large dynamic range dual-mode pH sensing.
Analysis of 15 anti-obesity drugs in urine using thermal-assisted paper spray mass spectrometry
Authors: Jiang Shijia , Zhao Junbo , Yan Hui , Xiang Ping , Shen Min
Published: August 2023

A thermal-assisted paper spray mass spectrometry method was developed to analyze anti-obesity drugs in urine based on an innovative investigation into the effect of the temperature of the paper substrate, accompanied by reduced limits of detection and higher sensitivity.
Highly efficient light-driven hydrogen evolution utilizing porphyrin-based nanoparticles
Authors: Nikolaou Vasilis , Agapaki Eleni , Nikoloudakis Emmanouil , Achilleos Katerina , Ladomenou Kalliopi , Charalambidis Georgios , Triantafyllou Evitina , Coutsolelos Athanassios G.
Published: August 2023

Dye-sensitized photocatalytic systems (DSPs) for H2 evolution were developed, by utilizing porphyrins as photosensitizer–catalyst (PS/CAT) entities onto platinum-doped titanium dioxide nanoparticles (Pt-TiO2 NPs).
Vitamin B12 and a metal–organic framework enable the photocatalytic generation of alkyl radicals
Authors: Dworakowski Krzysztof R. , Chołuj Artur , Chmielewski Michał J. , Gryko Dorota
Published: August 2023

Metal Organic Framework-MIL-125-NH2 enables the photochemical reduction of native vitamin B12 to the supernucleophilic Co(i) form. The newly developed system catalyzes the C–C bond-forming reaction and the dehalogenation of DDT.
Weakly coordinating tert-amide assisted Rh(iii)-catalyzed C4-cyanation of indoles: application in photophysical studies
Authors: Sarkar Souradip , Biswas Aniruddha , Das Sarbojit , Sanyal Bortika , Sahoo Rajkumar , Samanta Rajarshi
Published: August 2023

A rhodium(iii)-catalyzed indole C4-selective cyanation is described using the bench-stable, user-friendly electrophilic cyanation agent N-cyano-N-phenyl-p-toluenesulfonamide (NCTS) as a coupling partner.
Fused metallacyclopropenes from alkynylphenols
Authors: Fu Bingjie , Bai Wei , Zhao Yue , Li Yang , Jiang Wenfeng
Published: August 2023

Metallabenzofuran-fused osmacyclopropene complexes are obtained from the reactions of OsCl2(PPh3)3 with 2-alkynylphenols. Paramagnetic alkyne-coordinated phenolate Os complexes are proposed to be the key intermediates.
Reduction of esters to alcohols and iodides using aminodiborane (μ-NH2B2H5): scope and mechanistic investigations
Authors: Nair Abhishek , Tiwari Vikas , Rath Sambhav , Saini Parul , Verma Ashutosh , Elias Anil J.
Published: August 2023

Herein, we report an efficient methodology for the reduction of esters to alcohols and iodides using in situ generated aminodiborane from iodine and ammonia borane.
An amorphous Lewis-acidic zirconium chlorofluoride as HF shuttle: C–F bond activation and formation
Authors: Heinekamp Christian , Buzanich Ana Guilherme , Ahrens Mike , Braun Thomas , Emmerling Franziska
Published: August 2023

A process to shuttle HF from a fluoroalkane to alkynes has been developed. The consecutive dehydrofluorination and hydrofluorination steps are catalysed by an amorphous zirconium chlorofluoride at room temperature.
Computational and biophysical methods for the discovery and optimization of covalent drugs
Authors: Wang Guanyu , Moitessier Nicolas , Mittermaier Anthony K.
Published: August 2023

Iterative covalent drug design and discovery combining computational, synthetic, and biophysical chemistry.
Precise construction of weather-sensitive poly(ester-alt-thioesters) from phthalic thioanhydride and oxetane
Authors: Fornacon-Wood Christoph , Stühler Merlin R. , Gallizioli Cesare , Manjunatha Bhargav R. , Wachtendorf Volker , Schartel Bernhard , Plajer Alex J.
Published: August 2023

Copolymerisation of phthalic thioanhydride and oxetane yields high molecular weight poly(ester-alt-thioesters) which show degradation of the thioester links in artificial weathering experiments.
Single B-vacancy enriched α1-borophene sheet: an efficient metal-free electrocatalyst for CO2 reduction
Authors: Roy Prodyut , Ghoshal Sourav , Pramanik Anup , Sarkar Pranab
Published: August 2023

First principles calculations are performed to see the efficacy of pristine (α1) and different defective (α1−t1, α1−t2) borophene sheets as metal-free electrocatalysts for the CO2 reduction reaction.
Optical response and multi-exciton effects in 2D PTCDA aggregates with local excitation
Authors: Wei An , Fang Deyin , Lyu Siyuan , Zhang Yuan , Klamroth Tillmann , Wang Luxia
Published: August 2023

Dark state can be obtained by the single chain local excitation, and the red-shift Δ is related to the inter-chain coupling in PTCDA aggregate.
On the performance of the M–C (M = Fe, Ru, Os) unit toward methane activation
Authors: Li Shihan , Qian Chao , Wu Xiao-Nan , Zhou Shaodong
Published: August 2023

Gas-phase reactions of [MC]+ (M = Os and Ru) with methane at ambient temperature have been studied by using quadrupole-ion trap (Q-IT) mass spectrometry combined with quantum chemical calculations.
Going ballistic: a novel characterization for the electronic energy gap
Authors: Zhou Xuehua , Li Qingxia , Fang Yinyin , Xu Huan , Han Chao
Published: August 2023

Principle of HET operation. When the energy surpass the barrier (φ and Δ), hot carriers inject into the molecular levels. According to the measured LUMO/HOMO or φ/Δ, the energy gap of molecule can be determined.
Keeping the chromophores crossed: evidence for null exciton splitting
Authors: Lijina M. P. , Benny Alfy , Sebastian Ebin , Hariharan Mahesh
Published: August 2023

Attempts to identify and probe atypical molecular aggregates focusing on the less explored null aggregates. The null aggregate was foretold by Kasha to possess null exciton interaction leading to spectroscopically uncoupled molecular assembly.
Beryllium compounds for the carbon–halogen bond activation of phenyl halides: the role of non-innocent ligands
Authors: Trujillo-González Daniel E. , González-García Gerardo , Jiménez-Halla J. Oscar C. , Solà Miquel
Published: August 2023

A detailed DFT mechanistic study is presented to describe the phenyl–halogen bond activation mediated by five-membered heterocyclic Be(ii) bidentate ligands and (CAAC)2Be. Our study highlights the key role played by the ligands in this process.
A series of ligustrazine platinum(iv) complexes with potent anti-proliferative and anti-metastatic properties that exert chemotherapeutic and immunotherapeutic effects
Authors: Chen Yan , Li Linming , Liu Zhifang , Liu Meifeng , Wang Qingpeng
Published: August 2023

A series of ligustrazine platinum(iv) complexes were developed, which displayed potent anti-proliferative and anti-metastatic activities by causing DNA damage, mitochondrial damage, and pro-death autophagy and activating immune response.
Imparting structural robustness of metal–organic cages based on oxo-dimolybdenum clusters
Authors: Delaporte Solène , Abánades Lázaro Isabel , López-Cabrelles Javier , Mazarakioti Eleni C. , Chebourou Sarah , Vitórica-Yrezábal Iñigo J. , Giménez-Marqués Mónica , Mínguez Espallargas Guillermo
Published: August 2023

A family of robust and stable molybdenum-based metal–organic cages have been obtained based on the [Mo2O2(μ2-O)2]2+ secondary building unit.
Beneficial effect of ζ-carotene-like compounds on acute UVB irradiation by alleviating inflammation and regulating intestinal flora
Authors: Zhang Liping , Wang Kai , Liang Shaoxin , Cao Junhan , Yao Mengke , Qin Ling , Qu Changfeng , Miao Jinlai
Published: August 2023

ζ-Carotene is a key intermediate in the carotenoid pathway, but owing to its low content and difficulties in isolation, its application is restricted.
Catalyst-free electroreductive carboxylic acid–nitroarene coupling
Authors: Wang Qing , Xu Jia , Xu Zhimin , Wang Zhizhao , Tao Xiangzhang , Ni Shengyang , Pan Yi , Wang Yi
Published: August 2023

The cross-coupling of redox-active esters with nitroarenes has been achieved by electrosynthesis with sacrificial metal anodes.
Evaluating calcium phosphate glass phantoms for the calibration of in vivo X-ray fluorescence spectrometry-based methods of bone strontium quantification
Authors: Micsa Matthew , Da Silva Eric
Published: August 2023

Calcium phosphate glass phantoms were assessed as candidate solid-state calibrators for in vivo X-ray fluorescence spectrometry applications as stable substitutes for plaster of Paris and hydroxyapatite.
Analysis of geological glasses by electron probe microanalysis under low beam current density conditions
Authors: Zhu Ji-Hao , Chu Feng-You , Jochum Klaus Peter , Zhan Xiu-Chun , Ding Xing , Wu Bin , Lu Jiang-Gu , Li Yun-Xiu , Dong Yan-Hui , Liu Ji-Qiang , Wang Yu-Qi , Wu Shi-Tou
Published: August 2023

An electron probe microanalysis method was developed to mitigate alkali ion migration effects under low beam current density conditions with a time-saving mode to collect alkali-ion X-ray signals. The method is suitable for estimating H2O content in H2O-rich geological glasses as a substitute technique for SIMS and FTIR.
Is AI essential? Examining the need for deep learning in image-activated sorting of Saccharomyces cerevisiae
Authors: Hayashi Mika , Ohnuki Shinsuke , Tsai Yating , Kondo Naoko , Zhou Yuqi , Zhang Hongqian , Ishii Natsumi Tiffany , Ding Tianben , Herbig Maik , Isozaki Akihiro , Ohya Yoshikazu , Goda Keisuke
Published: August 2023

We experimentally justify the advantages of jumping on the deep learning trend for image-activated budding yeast sorting and validate its applicability towards morphology-based yeast mutant screening.
Recent progress in the development of conductive hydrogels and the application in 3D printed wearable sensors
Authors: Lin Yuxuan , Yang Ruxue , Wu Xin
Published: August 2023

This paper reviewed the research progress of 3D printing in wearable sensors based on conductive hydrogels, with an emphasis on classification of conductive hydrogels, the mechanisms of wearable sensors and applications of 3D printing methods.
Electron transfer bridge inducing polarization of nitrogen molecules for enhanced photocatalytic nitrogen fixation
Authors: Li Huiyi , Wang Jiongrong , Ruan Zhoushilin , Nan Pengfei , Ge Binghui , Cheng Ming , Yang Lan , Li Xiaohong , Liu Qilong , Pan Bicai , Zhang Qun , Xiao Chong , Xie Yi
Published: August 2023

Employing rare earth metal atoms as an electron transfer bridge, directional polarization was achieved for inert nitrogen molecules, leading to a significant enhancement in photocatalytic nitrogen fixation.
Manufacture of highly loaded Ni catalysts by carbonization–oxidation–reduction for dry reforming of methane
Authors: Bai Yue , Shen Dongyang , Yu Guowang , Wang Jie , Lyu Shuai , Zhang Yuhua , Wang Guanghui , Li Jinlin , Li Lin
Published: August 2023

Highly loaded Ni catalysts with dual size distribution fabricated by COR pretreatment promote carbon dioxide reforming of methane reaction.
Palladium-catalyzed convenient synthesis of thioesters from carboxylic acids and disulfides
Authors: Xiao Yong-Mei , Zhao Yu , Li Jia-Qi , Yuan Jin-Wei , Yang Liang-Ru , Mao Pu , Mai Wen-Peng
Published: August 2023

A novel palladium-catalyzed convenient synthesis of thioesters from various carboxylic acids and disulfides has been developed.
Ternary organic solar cells with a selenophene-containing donor guest achieve a high efficiency of 18.41%
Authors: Li Zhengjin , Liu Chunyan , Liu Jianhua , Long Chengjiang , Chen Yun , Huang Xun , Wei Luhong , Qiu Nailiang
Published: August 2023

A selenophene-containing and chlorinated benzodithiophene based guest donor endows the ternary organic solar cells with a high efficiency of 18.41%.
Why is the ground state of m-benzoquinone a triplet?
Authors: Leyva-Parra Luis , Pino-Rios Ricardo
Published: August 2023

The electronic structure, changes in aromatic behavior, and stability of the m-quinone, the least studied member of the benzoquinone family, have been analyzed.
A hyper-reflective cholesteric liquid crystal polymer network with double layers
Authors: Zhang Ming , Zhao Jinghua , Yao Zhigang , Liu Wei , Li Yi , Yang Yonggang
Published: August 2023

Hyper-reflective double-layered cholesteric liquid crystal network films were prepared, which can be applied for decoration and anti-counterfeiting.
Minimizing base stoichiometry in Pd(0)/g-C3N4O catalyzed Suzuki–Miyaura cross-coupling reaction
Authors: Konwar Dipika , Saikia Rakhee , Kalita Risha , Das Manash R. , Bora Utpal
Published: August 2023

A Pd nanoparticle supporting graphitic carbon nitride oxide (g-C3N4O) sheet is developed as an efficient heterogeneous catalyst for Suzuki–Miyaura cross-coupling reaction requiring just under a substoichiometric amount of exogenous base.
Thiophene BODIPY-substituted cyclotriphosphazene-derived photosensitizers for photodynamic therapy applications
Authors: Yıldız Gül Elif , Erdem Murat , Kazan Hasan Hüseyin , Tanrıverdi Eçik Esra
Published: August 2023

Molecular design and synthesis of cyclotriphosphazene-BODIPY-based PSs based on a halogen atom-enhanced ISC process were carried out and their photophysical behaviors, ROS production abilities and finally in vitro PDT potentials were investigated.
Regiodivergent sulfonylation of terminal olefins via dearomative rearrangement
Authors: Blé-González Ever A. , Isbel Stephen R. , Ojo Olatunji S. , Hillesheim Patrick C. , Zeller Matthias , Bugarin Alejandro
Published: August 2023

A regiodivergent transition metal-free approach towards sulfones [(E)-allylic sulfones and α-sulfonylmethyl styrenes] is reported. Solvents and temperature play a key role in the selectivity of the reaction.
Humidity and ultraviolet modulate color turned LnNa-based metal–organic frameworks as Bi-decryption anti-counterfeit materials
Authors: Wang Jinzeng , Cui Mengjuan , Yao Haiyun , Wang Fang , Li Xiaolong
Published: August 2023

Ultra-violet and water atomization mudulated dynamic anti-counterfeit LnMOF's luminescent inks with enhanced luminescent intensity at 50% Gd dilution.
Macroscopic alignment of metal–organic framework crystals in specific crystallographic orientations
Authors: Park Jonghoon , Moon Hoi Ri , Kim Jin Yeong
Published: August 2023

This review explores synthetic strategies for macroscopic aligned MOFs, enhancing properties without structural designs or post-synthetic reactions.
A practical approach for oligopeptide synthesis via synergistic photoredox, cobaloxime and organophosphorus triple catalysis
Authors: Su Junqi , Mo Jia-Nan , Zhang Guofeng , Jiang Ziyu , Zhao Jiannan
Published: August 2023

A catalytic oxidation–reduction condensation strategy for the synthesis of oligopeptides is reported. The triple catalytic system is compatible with all proteinogenic amino acids and applicable for multigram scale peptide synthesis.
Metal-free photocatalyzed allylic silylation of allyl acetates and chlorides
Authors: Yu Xin-Long , Hu Jia-Wei , Cao Jian , Xu Li-Wen
Published: August 2023

A visible light-induced organophotocatalytic strategy for synthesizing functionalized allylsilanes through radical allylic silylation of allyl acetates and chlorides is reported.
Hydrogenation of citral and carvone on Pt and PtSn supported metallic catalysts. A comparative study on the regioselectivity and chemoselectivity
Authors: Ramos Montero Gustavo Enrique , Stassi Julieta Paola , de Miguel Sergio Rubén , Zgolicz Patricia Daniela
Published: August 2023

Pt and PtSn catalysts supported on oxide and carbonaceous materials have been studied for the regioselective and chemoselective hydrogenation of citral and carvone.
Green synthesis of the copper and iron phthalocyanine-based metal–organic framework as an efficient catalyst for methylene blue dye degradation and oxidation of cyclohexane
Authors: Bhise Rupali S. , Patil Yogesh A. , Shankarling Ganapati S.
Published: August 2023

A heterogeneous catalyst with high recyclability, good conversion, less reaction time, and low temperature.
Transition from Dion–Jacobson hybrid layered double perovskites to 1D perovskites for ultraviolet to visible photodetection
Authors: Mandal Arnab , Gupta Shresth , Dutta Supriti , Pati Swapan K. , Bhattacharyya Sayan
Published: August 2023

New lead-free perovskite phases with varying dimensionalities render them with diverse optoelectronic properties from the ultraviolet to visible wavelength range.
Design rules for reciprocal coupling in chemically fueled assembly
Authors: Chen Xiaoyao , Kriebisch Brigitte A. K. , Bergmann Alexander M. , Boekhoven Job
Published: August 2023

Chemical reaction cycles can regulate molecular self-assembly. Vice versa, self-assembly can regulate reaction rates. Here, we show design rules to tune such reciprocal coupling in dissipative self-assembly.
A natural polyphenol activates and enhances GPX4 to mitigate amyloid-β induced ferroptosis in Alzheimer's disease
Authors: Baruah Prayasee , Moorthy Hariharan , Ramesh Madhu , Padhi Dikshaa , Govindaraju Thimmaiah
Published: August 2023

Tannic acid (TA) mitigates ferroptosis induced by amyloid β-Fe in Alzheimer's disease (AD) through a multipronged activity. TA activates and enhances the GPX4 levels to counteract the complex interaction between AD and ferroptosis.
Metal-free, photoinduced remote C(sp3)–H borylation
Authors: He Jiachen , Cook Silas P.
Published: August 2023

Described herein is a protocol for the metal-free, photo-induced borylation of unactivated C(sp3)–H bonds γ to an O-oxalate hydroxamic ester functionality.
The immunology of heart failure with preserved ejection fraction
Authors: Smart Charles Duncan , Madhur Meena S.
Published: August 2023

Heart failure with preserved ejection fraction (HFpEF) now accounts for the majority of new heart failure diagnoses and continues to increase in prevalence in the United States. Importantly, HFpEF is a highly morbid, heterogeneous syndrome lacking effective therapies. Inflammation has emerged as a potential contributor to the pathogenesis of HFpEF. Many of the risk factors for HFpEF are also associated with chronic inflammation, such as obesity, hypertension, aging, and renal dysfunction. A large amount of preclinical evidence suggests that immune cells and their associated cytokines play important roles in mediating fibrosis, oxidative stress, metabolic derangements, and endothelial dysfunction, all potentially important processes in HFpEF. How inflammation contributes to HFpEF pathogenesis, however, remains poorly understood. Recently, a variety of preclinical models have emerged which may yield much needed insights into the causal relationships between risk factors and the development of HFpEF, including the role of specific immune cell subsets or inflammatory pathways. Here, we review evidence in animal models and humans implicating inflammation as a mediator of HFpEF and identify gaps in knowledge requiring further study. As the understanding between inflammation and HFpEF evolves, it is hoped that a better understanding of the mechanisms underlying immune cell activation in HFpEF can open up new therapeutic avenues.
Comprehensive Assessments of Theory of Mind Abilities for School-Age Children: A Systematic Review
Authors: Poll Gerard H. , Drexler Sherilyn , Huntington Allison , Galindo Mackenzie , Galack Alexa
Published: August 2023

Purpose:

Theory of mind involves perceiving both the thoughts and emotions of others. Theory of mind skills continue to develop in school-age individuals. For children at risk for social communication difficulties, evaluating theory of mind is warranted to determine whether theory of mind should be a target of intervention. There are hundreds of tasks designed to evaluate theory of mind, but many evaluate a single facet of the construct and have uneven psychometric properties. Comprehensive assessments evaluate both cognitive and affective aspects of theory of mind. The purpose of this systematic review was to identify comprehensive theory of mind assessments for school-age individuals and to critically appraise their measurement properties.

Method:

Four databases were searched to identify 27 included records describing the measurement properties of comprehensive theory of mind assessments for 5- to 21-year-olds. Assessments appearing in at least three records were included. The methodological quality of the studies and the sufficiency of assessments' measurement properties were evaluated using the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN).

Results:

Nine assessments were evaluated, and four met COSMIN standards for sufficient content validity and internal consistency to be recommended for clinical use.

Conclusions:

Comprehensive assessments enable examiners to develop a profile of children's theory of mind strengths and needs. Strengths of the identified assessments include sufficient convergent validity and internal consistency. Further research is required to develop higher quality evidence for the content validity, structural validity, and reliability of the included assessments.

Supplemental Material:

https://doi.org/10.23641/asha.23817495