bluuebunny/crossref_metadata_2025 huggingface dataset:
Artificial Light at Night (ALAN) Influences Understory Plant Traits through Ecological Processes: A Two-Year Experiment in a Rubber Plantation in China
Authors: Zhou Cong , Nakamura Akihiro , Song Xiaoyang , Katabuchi Masatoshi
Published: November 2023

Artificial light at night (ALAN) demonstrated a new ecological factor that influences organisms through a multi-approach. Yet, the impacts of ALAN on understory plants remain largely unknown. We evaluated whether ALAN would affect the leaf mass per area (LMA) of understory plants through a two-year field light experiment in a tropical rubber plantation in south China. We hypothesized that ALAN could impact the understory in two ways: by directly supplementing light to aboveground plant parts (which increases LMA) and indirectly affecting soil nutrient composition by attracting insects (which decreases LMA). We selected two species: Colocasia gigantea, representing shade-tolerant species, and Melastoma candidum, representing light-demanding species. We measured canopy openness, LMA, soil nutrients, and individual distance away from light resources. Our Bayesian linear mixed model showed a negative relationship between LMA and the strength of ALAN, indicating that ALAN may influence LMA more indirectly by enhancing soil nutrient availability rather than directly acting as a light resource. This relationship was significant for Colocasia gigantea but not for Melastoma candidum. These results suggest that ALAN might have complex and species-specific impacts on the understory ecosystem. Our study underscores the need for continued research and informed management of anthropogenic ecosystems.
Future Scenarios of Firewood Consumption for Cooking in the Mexican Tropical Region
Authors: Pérez Guadalupe , Grande-Acosta Genice K. , Islas-Samperio Jorge M. , Manzini Fabio
Published: November 2023

Within domestic food cooking, burning firewood in three-stone fires (TSF) is a common practice by more than 16 million users in the Mexican tropical climate region (CR-TR). This article aims to evaluate the implementation of improved firewood cookstoves (ICS) to replace TSF in the CR-TR by constructing firewood consumption scenarios covering 2018–2050. The results show that in CR-TR, with the implementation of ICS, the consumption of 354.95 PJ of firewood, 36.6 Mt of CO2e, 1.29 Mt of CO, and 163.78 kt of PM2.5 can all be avoided. The most important reduction in firewood consumption, CO2e emissions and CO and PM2.5 pollutants, occurs in exclusive firewood users and mixed users who utilize firewood as the primary fuel source, both of whom are low socioeconomic level in rural areas. Furthermore, most paying-for-firewood users often show negative mitigation costs and a high 50% IRR, while all non-paying-for-firewood users have mitigation costs ranging from 7.74 to 41.23 USD/tCO2e. At the end of the results section, we perform a sensitivity analysis of the relevant parameters, which complements this study. Therefore, implementing ICS will contribute to the solution of climate change, deforestation, and facilitate the formulation of sustainable development policies for the most vulnerable population sector of the Mexican CR-TR.
Numerical Investigation on Mesoscale Evolution of Hydraulic Fractures in Hydrate-Bearing Sediments
Authors: Liang Xiaowei , Zhao Hui , Dang Yongchao , Lei Qihong , Wang Shaoping , Wang Xiaorui , Chai Huiqiang , Jia Jianbo , Wang Yafei
Published: November 2023

Hydraulic fracturing is widely recognized as a potential stimulation technology for the development of challenging natural gas hydrate. However, the fracturing behavior of non-diagenetic hydrate reservoirs has peculiar characteristics that are different from those of conventional oil and gas reservoirs. Herein, a fully coupled fluid-mechanical model for simulating hydraulic fracturing in hydrate-bearing sediments (HBS) was established based on the discrete element method, and the influence of hydrate saturation, in situ stress, and injection rate on the meso-fracture evolution was investigated. The results indicate that with the increase in hydrate saturation, the fracture morphology transitions from bi-wing to multi-branch, thereby enhancing fracture complexity. Both tensile and shear failure modes exist, and the tensile failure between the weakly cemented sediment particles is dominant. The tensile strength of HBS is an exponential function of hydrate saturation, with the breakdown pressure being governed by hydrate saturation and in situ stress, with the form being consistent with the classical Kirsch equation. Additionally, lower in situ stress and higher injection rates are conducive to the generation of microcracks, whereas an excessive injection rate reduces the fracture length. These findings contribute to understanding the meso-evolution mechanism of hydraulic fractures and guide the design of on-site hydraulic fracturing plans of natural gas hydrate reservoirs.
Development of a Novel Control Scheme to Achieve the Minimum Unbalance Factor and Real Power Fluctuations under Asymmetrical Faults
Authors: Abubakar Muhammad , Renner Herwig , Schürhuber Robert
Published: November 2023

The increasing share of converter-based renewable energy sources in the power system has forced the system operators to demand voltage support from the converters in case of faults. In the case of symmetric faults, all the phases have equal voltage support, but in the case of asymmetric faults, selective voltage support is required. The grid codes define the voltage support required in the case of symmetric/asymmetric faults, which is the reactive current injection in the respective sequence proportional to its voltage dip, but studies confirm that it does not result in a minimum unbalance factor in the case of asymmetric faults. The unbalance factor is an indication of the level of imbalance voltage among the phases. Moreover, it also results in fluctuated active power injection in the case of asymmetric faults, which causes dc link voltage fluctuations, and the power reversal may also occur due to such fluctuations, which leads to higher protection costs for the dc link. In order to (1) enhance the uniformity of voltage among different phases in the case of asymmetric faults and (2) minimize the real power fluctuations in such conditions, a novel control scheme is presented in this paper. It optimally distributes the negative sequence current phasor into its active and reactive components to achieve the minimum voltage unbalance factor. It also confirms the minimum real power fluctuations by adjusting the positive and negative sequence current phasors. The proposed scheme also ensures the current limit of the converter. The proposed scheme is developed in Matlab/Simulink and tested under different faulty conditions. The results confirm the better performance of the proposed scheme against the grid code recommendation under different faulty conditions.
Prevalence and Trends of Basic Activities of Daily Living Limitations in Middle-Aged and Older Adults in the United States
Authors: Heimbuch Halli , Rhee Yeong , Douglas Marty , Juhl Kirsten , Knoll Kelly , Stastny Sherri , McGrath Ryan
Published: November 2023

Background: Population-level surveillance of the prevalence and trends of basic self-care limitations will help to identify the magnitude of physical disablement in the rapidly growing older American demographic. We sought to evaluate the prevalence and trends of activities of daily living (ADL) limitations in the United States. Methods: The analytic sample included 30,418 Americans aged ≥50 years from the 2006–2018 waves of the Health and Retirement Study. ADLs were self-reported. Weighted prevalence estimates were presented, and trends analyses were performed. Results: Although overall ADL disability prevalence was 16.5% (95% confidence interval: 15.8–17.2) in 2018, there were no changes in limitations during the study period (p = 0.52). Older adults had a greater ADL disability prevalence than middle-aged adults (p < 0.001). While older persons experienced a declining trend of ADL limitations (p < 0.001), middle-aged persons had an increasing trend (p < 0.001). Males had a lower ADL limitation prevalence than females (p < 0.001). Hispanic and non-Hispanic Black had a higher ADL disability prevalence than non-Hispanic White (p < 0.001). Conclusions: This investigation revealed that while the estimated prevalence of ADL limitations in the United States was substantial, changes in such limitations were not observed. Our findings can help guide ADL screening, target sub-populations with an elevated ADL limitation prevalence, and inform interventions.
Soil Enzyme Activity Differs among Native Species and Continuously Planted Eucalyptus Plantations
Authors: Cheng Yajun , Du Apeng , Wang Zhichao , Zhu Wankuan , Ren Shiqi , Xu Yuxing , Ren Shifu
Published: November 2023

In recent years, monoculture and multi-rotation successional Eucalyptus plantations have given rise to several environmental issues, including the degradation of soil quality and nutrient imbalance, and the conversion of logging sites to multi-rotation Eucalyptus plantations has attracted considerable attention from the scientists involved. However, the effects of different management strategies on soil extracellular enzyme activities (EEAs) and enzyme stoichiometry (ES) in degraded Eucalyptus plantations are not clear. In this study, we investigated the responses and mechanisms of soil physicochemical properties, microbial biomass, carbon, and nitrogen- and phosphorus-acquiring enzyme activities, as well as the microbial resource requirements of Eucalyptus plantations, under different management strategies. The findings revealed that second-rotation (TWE) and third-rotation (THE) continuous plantings of pure Eucalyptus plantations resulted in significant decreases in soil organic carbon (SOC), total nitrogen (TN) and effective available phosphorus (AP) contents, while soil nutrient contents increased after the introduction of Manglietia glauca to form mixed forests (EM) with Eucalyptus or pure Manglietia glauca (M). Meanwhile, phosphorus-acquiring enzymes significantly increased with successive rotations of Eucalyptus (TWE and THE), while EEAC:P and EEAN:P gradually decreased and phosphorus limitation gradually increased compared to that of a native-species-mixed plantation (CK). After the introduction of Manglietia glauca (EM and M), phosphorus-acquiring enzyme activities showed lower levels and there were significant increases in EEAC:P and EEAN:P compared to those of continuous plantings of pure Eucalyptus plantations, which reduced microbial phosphorus demand. Moreover, soil nutrients played a more significant role in altering the EEAs and ES than did microbial biomass (0–10 cm: 72.7% > 53.3%, 10–20 cm: 54.5% > 32.6%). The results showed that EM and M improved soil fertility quality conditions and alleviated soil nutrient phosphorus limitations for soil microorganisms. Therefore, the introduction of Manglietia glauca, either to form mixed forests with Eucalyptus or in rotation with Eucalyptus, can be used as technical means for the conversion of multi-rotation successive Eucalyptus plantations.
Development of Spatiotemporal Whole-Stem Models for Estimating End-Product-Based Fibre Attribute Determinates for Jack Pine and Red Pine
Authors: Newton Peter F.
Published: November 2023

The objective of this study was to develop spatiotemporal whole-stem wood quality prediction models for a suite of end-product-based fibre attribute determinates for jack pine (Pinus banksiana Lamb.) and red pine (Pinus resinosa Aiton): specifically, for wood density (Wd), microfibril angle (Ma), modulus of elasticity (Me), fibre coarseness (Co), tracheid wall thickness (Wt), tracheid radial diameter (Dr), tracheid tangential diameter (Dt), and specific surface area (Sa). Procedurally, these attributes were determined for each annual ring within pith-to-bark xylem sequences extracted from 610 jack pine and 223 red pine cross-sectional disks positioned throughout the main stem of 61 jack pine and 54 red pine sample trees growing within even-aged monospecific stands in central Canada. Deploying a block cross-validation-like approach in order to reduce serial data dependency and enable predictive performance assessments, species-specific calibration and validation data subsets consisting of cumulative moving average values were systematically generated from the 27,820 jack pine and 11,291 red pine attribute-specific annual ring values. Graphical, correlation, regression and validation analyses were used to specify, parameterize and assess the predictive performance of tertiary-level (ring-disk-tree) hierarchical mixed-effects whole-stem equations for each attribute by species. As a result, the jack pine equations explained 46, 66, 74, 63, 59, 72, 42 and 48% of the variation in Wd, Ma, Me, Co, Wt, Dr, Dt and Sa, respectively. The red pine equations explained slightly higher levels of variation except for Me: 50, 71, 31, 83, 72, 78, 56 and 71% of the variation in Wd, Ma, Me, Co, Wt, Dr, Dt and Sa, respectively. Graphical assessments and statistical metrics related to attribute and species-specific residual error patterns and goodness-of-fit, lack-of-fit and predictive error metrics, revealed an absence of systematic bias, misspecification or aberrant predictive performance. Consequently, the resultant parameterized models were acknowledged as acceptable functional descriptors of the intrinsic spatiotemporal cumulative developmental patterns of the studied end-product fibre attribute determinates, for these two pine species. Although predicted development patterns were similar between the species with the greatest degree of nonlinearity occurring before a cambial age of approximately 30 years, irrespective of attribute, jack pine exhibited a greater degree of nonlinearity in the Wd and Dt developmental trajectories, whereas red pine exhibited a greater degree of nonlinearity in the Ma, Me, Co, Wt, Dr and Sa developmental trajectories. Potential biomechanical linkages underlying the observed attribute distribution patterns, as well as the potential utility of the models in forest management, are also discussed.
Experimental Investigation on Dowel Laminated Timber Made of Uruguayan Fast-Grown Species
Authors: Bruzzone Gastón , Godoy Daniel , Quagliotti Sebastián , Arrejuría Stephany , Böthig Silvia , Moya Laura
Published: November 2023

This study aims to evaluate the structural behaviour of dowel laminated timber (DLT) made of fast-growing pine and eucalyptus lamellae and different dowel diameters. Shear tests on dowelled connections and four-point bending tests on structural size panels were performed, and the slip modulus and bending properties, respectively, were obtained. The bending stiffness and strength of pine panels fastened with guatambú (5.05 and 20.05 kN/mm2) or eucalyptus dowels (5.29 and 17.13 kN/mm2), suggesting that dowel species had no influence on the panel’s bending properties. The analysis of the bending stiffness and strength of eucalyptus panels fastened with 18 mm (11.04 and 44.29 kN/mm2) or with 15 mm dowel diameter (11.06 and 51.50 kN/mm2) indicated that dowel diameter had no substantial influence on the bending properties. Furthermore, the stiffness values of the panels were similar to those of their constitutive individual lamellas, which, in turn, did not comply with the structural requirements of the Uruguayan Standard. Additional analytical results of the effective stiffness using the slip modulus were close to those found in the experimental bending tests. The results from this study are useful in understanding the mechanical behaviour of DLT panels and provide the basis for the improvement of Uruguayan mass timber products.
Effect of High-Hydrostatic-Pressure Treatment on the Physicochemical Properties of Kafirin
Authors: Yang Yajing , Chen Qiongling , Liu Qingshan , Wang Xiaowen , Bai Wenbin , Chen Zhenjia
Published: November 2023

The kafirin derived from Jin Nuo 3 sorghum underwent a high-hydrostatic-pressure (HHP) treatment of 100, 300, and 600 MPa for 10 min to investigate alterations in its physicochemical attributes. The findings exhibited a reduction in protein solubility, declining from 83% to 62%, consequent to the application of the HHP treatment. However, this treatment did not lead to subunit-specific aggregation. The absorption intensity of UV light diminished, and the peak fluorescence absorption wavelength exhibited a shift from 342 nm to 344 nm, indicating an increased polarity within the amino acid microenvironment. In an aqueous solution, the specific surface area expanded from 294.2 m2/kg to 304.5 m2/kg, while the average particle-size value in a 70% ethanol solution rose to 26.3 nm. Conversely, the zeta-potential value decreased from 3.4 mV to 1.3 mV, suggesting a propensity for aggregation in ethanol solutions. A notable rise in the intermolecular β-sheet content to 21.06% was observed, along with a shift in the peak denaturation temperature from 76.33 °C to 86.33 °C. Additionally, the content of disulfide bonds increased to 14.5 μmol/g. Collectively, the application of the HHP treatment not only enhanced the thermal stability but also induced a more ordered secondary structure within the kafirin.
Sternal Rib Ends as a Method of Age Estimation at the CIL: A Brief Note
Authors: Christensen Alexander F.
Published: November 2023

This communication reports the relationship between skeletal age estimates based on the sternal rib ends (using the phase method of İşcan and colleagues, later revised by Hartnett) and the chronological age at the death of 221 U.S. military casualties processed by the Scientific Analysis section of the Defense POW/MIA Accounting Agency and its predecessor laboratories from 2000 to the present. Previously published age ranges for each phase do not provide accurate estimates for a sufficient proportion of the cases for forensic use; as an example, the age ranges that accompany reference cast sets proved accurate for 55% of CIL cases. Combining the published age ranges of İşcan and Hartnett, on the other hand, proved accurate in 99% of the cases.
A Prognathodontin Mosasaur from the Maastrichtian of the Dakhla Oasis, Western Desert, Egypt
Authors: El-Kheir Gebely A. Abu , Shaker Ahmed A. , Street Hallie P. , Longrich Nicholas R. , Strougo Amin , Asan Anhar , AbdelGawad Mohamed
Published: November 2023

Mosasaurs were diverse in the Upper Cretaceous in Africa, but relatively little is known about the mosasaur fauna of Egypt. Here, associated teeth and postcranial skeletal elements are reported for a mosasaur from the Maastrichtian Dakhla Shale of the Dakhla Oasis. The specimen includes tooth crowns, cervical, dorsal, and caudal vertebrae, and ribs. Teeth and bones exhibit features allowing referral to Prognathodontini. The teeth are relatively straight and blunt, suggesting affinities with Prognathodon overtoni or P. currii. Prognathodontins were important predators in the Maastrichtian of Africa, previously being recorded in Morocco, Congo, and Angola.
Morphological Evaluation of Supramolecular Soft Materials Obtained through Co-Assembly Processes
Authors: Croitoriu Alexandra , Chiriac Aurica P. , Rusu Alina G. , Ghilan Alina , Ciolacu Diana E. , Stoica Iuliana , Nita Loredana E.
Published: November 2023

Low-molecular-weight gelators (LMWGs) are compounds with an intrinsic tendency to self-assemble forming various supramolecular architectures via non-covalent interactions. Considering that the development of supramolecular assemblies through the synergy of molecules is not entirely understood at the molecular level, this study introduced a Fmoc-short peptide and four Fmoc-amino acids as building blocks for the self-assembly/co-assembly process. Hence, we investigated the formation of supramolecular gels starting from the molecular aggregation following two triggering approaches: solvent/co-solvent method and pH switch. The complex morphological analysis (POM, AFM, and STEM) offered an insight into the spontaneous formation of well-ordered nanoaggregates. Briefly, POM and AFM images demonstrated that self-assembled gels present various morphologies like dendrimer, spherulite, and vesicle, whereas all co-assembled supramolecular systems exhibit fibrillar morphologies as a result of the interaction between co-partners of each system. STEM study has confirmed that the molecules interact and join together, finally forming a fibrous network, an aspect seen in both self-assembled and co-assembled gels. XRD allowed the determination of the molecular arrangement. The study emphasized that the Fmoc motif protected the amino groups and facilitated gelation through additional π-π interactions.
Impaired Angiogenic Function of Fetal Endothelial Progenitor Cells via PCDH10 in Gestational Diabetes Mellitus
Authors: Kwon Hayan , Jung Yun Ji , Lee Yeji , Son Ga-Hyun , Kim Hyun Ok , Maeng Yong-Sun , Kwon Ja-Young
Published: November 2023

Maternal hyperglycemia, induced by gestational diabetes mellitus (GDM), has detrimental effects on fetal vascular development, ultimately increasing the risk of cardiovascular diseases in offspring. The potential underlying mechanisms through which these complications occur are due to functional impairment and epigenetic changes in fetal endothelial progenitor cells (EPCs), which remain less defined. We confirm that intrauterine hyperglycemia leads to the impaired angiogenic function of fetal EPCs, as observed through functional assays of outgrowth endothelial cells (OECs) derived from fetal EPCs of GDM pregnancies (GDM-EPCs). Notably, PCDH10 expression is increased in OECs derived from GDM-EPCs, which is associated with the inhibition of angiogenic function in fetal EPCs. Additionally, increased PCDH10 expression is correlated with the hypomethylation of the PCDH10 promoter. Our findings demonstrate that in utero exposure to GDM can induce angiogenic dysfunction in fetal EPCs through altered gene expression and epigenetic changes, consequently increasing the susceptibility to cardiovascular diseases in the offspring of GDM mothers.
Mono- and Dimeric Sorbicillinoid Inhibitors Targeting IL-6 and IL-1β from the Mangrove-Derived Fungus Trichoderma reesei BGRg-3
Authors: Liu Yufeng , Chen Tao , Sun Bing , Tan Qi , Ouyang Hui , Wang Bo , Yu Huijuan , She Zhigang
Published: November 2023

Four new sorbicillinoids, named trichodermolide E (1), trichosorbicillin J (2), bisorbicillinolide B (3), and demethylsorbiquinol (5), together with eight known compounds (4, 6–12), were isolated from the cultures of the mangrove-derived fungus Trichoderma reesei BGRg-3. The structures of the new compounds were determined by analyzing their detailed spectroscopic data, while the absolute configurations were further determined through electronic circular dichroism calculations. Snatzke’s method was additionally used to determine the absolute configurations of the diol moiety in 1. In a bioassay, compounds 7 and 10 performed greater inhibitory activities on interleukin-6 and interleukin-1β than the positive control (dexamethasone) at the concentration of 25 μM. Meanwhile, compounds 5 and 6 showed potent effects with stronger inhibition than dexamethasone on IL-1β at the same concentration.
M6A Demethylase Inhibits Osteogenesis of Dental Follicle Stem Cells via Regulating miR-7974/FKBP15 Pathway
Authors: Zheng Linwei , Li Zhizheng , Wang Bing , Sun Rui , Sun Yuqi , Ren Jiangang , Zhao Jihong
Published: November 2023

N6-methyladenosine (m6A) is the most abundant RNA modification, regulating gene expression in physiological processes. However, its effect on the osteogenic differentiation of dental follicle stem cells (DFSCs) remains unknown. Here, m6A demethylases, the fat mass and obesity-associated protein (FTO), and alkB homolog 5 (ALKBH5) were overexpressed in DFSCs, followed by osteogenesis assay and transcriptome sequencing to explore potential mechanisms. The overexpression of FTO or ALKBH5 inhibited the osteogenesis of DFSCs, evidenced by the fact that RUNX2 independently decreased calcium deposition and by the downregulation of the osteogenic genes OCN and OPN. MiRNA profiling revealed that miR-7974 was the top differentially regulated gene, and the overexpression of m6A demethylases significantly accelerated miR-7974 degradation in DFSCs. The miR-7974 inhibitor decreased the osteogenesis of DFSCs, and its mimic attenuated the inhibitory effects of FTO overexpression. Bioinformatic prediction and RNA sequencing analysis suggested that FK506-binding protein 15 (FKBP15) was the most likely target downstream of miR-7974. The overexpression of FKBP15 significantly inhibited the osteogenesis of DFSCs via the restriction of actin cytoskeleton organization. This study provided a data resource of differentially expressed miRNA and mRNA after the overexpression of m6A demethylases in DFSCs. We unmasked the RUNX2-independent effects of m6A demethylase, miR-7974, and FKBP15 on the osteogenesis of DFSCs. Moreover, the FTO/miR-7974/FKBP15 axis and its effects on actin cytoskeleton organization were identified in DFSCs.
Complete Chloroplast Genome of Hypericum perforatum and Dynamic Evolution in Hypericum (Hypericaceae)
Authors: Liu Xinyu , Bai Yuran , Wang Yachao , Chen Yifeng , Dong Wenpan , Zhang Zhixiang
Published: November 2023

Hypericum perforatum (St. John’s Wort) is a medicinal plant from the Hypericaceae family. Here, we sequenced the whole chloroplast genome of H. perforatum and compared the genome variation among five Hypericum species to discover dynamic changes and elucidate the mechanisms that lead to genome rearrangements in the Hypericum chloroplast genomes. The H. perforatum chloroplast genome is 139,725 bp, exhibiting a circular quadripartite structure with two copies of inverted repeats (IRs) separating a large single-copy region and a small single-copy region. The H. perforatum chloroplast genome encodes 106 unique genes, including 73 protein-coding genes, 29 tRNAs, and 4 rRNAs. Hypericum chloroplast genomes exhibit genome rearrangement and significant variations among species. The genome size variation among the five Hypericum species was remarkably associated with the expansion or contraction of IR regions and gene losses. Three genes—trnK-UUU, infA, and rps16—were lost, and three genes—rps7, rpl23, and rpl32—were pseudogenized in Hypericum. All the Hypericum chloroplast genomes lost the two introns in clpP, the intron in rps12, and the second intron in ycf3. Hypericum chloroplast genomes contain many long repeat sequences, suggesting a role in facilitating rearrangements. Most genes, according to molecular evolution assessments, are under purifying selection.
Extracellular Vesicles and Their Role in Lung Infections
Authors: Hambo Shadi , Harb Hani
Published: November 2023

Lung infections are one of the most common causes of death and morbidity worldwide. Both bacterial and viral lung infections cause a vast number of infections with varying severities. Extracellular vesicles (EVs) produced by different cells due to infection in the lung have the ability to modify the immune system, leading to either better immune response or worsening of the disease. It has been shown that both bacteria and viruses have the ability to produce their EVs and stimulate the immune system for that. In this review, we investigate topics from EV biogenesis and types of EVs to lung bacterial and viral infections caused by various bacterial species. Mycobacterium tuberculosis, Staphylococcus aureus, and Streptococcus pneumoniae infections are covered intensively in this review. Moreover, various viral lung infections, including SARS-CoV-2 infections, have been depicted extensively. In this review, we focus on eukaryotic-cell-derived EVs as an important component of disease pathogenesis. Finally, this review holds high novelty in its findings and literature review. It represents the first time to cover all different information on immune-cell-derived EVs in both bacterial and viral lung infections.
Ketogenic Diet Regulates Cardiac Remodeling and Calcium Homeostasis in Diabetic Rat Cardiomyopathy
Authors: Lee Ting-I , Trang Nguyen Ngoc , Lee Ting-Wei , Higa Satoshi , Kao Yu-Hsun , Chen Yao-Chang , Chen Yi-Jen
Published: November 2023

A ketogenic diet (KD) might alleviate patients with diabetic cardiomyopathy. However, the underlying mechanism remains unclear. Myocardial function and arrhythmogenesis are closely linked to calcium (Ca2+) homeostasis. We investigated the effects of a KD on Ca2+ homeostasis and electrophysiology in diabetic cardiomyopathy. Male Wistar rats were created to have diabetes mellitus (DM) using streptozotocin (65 mg/kg, intraperitoneally), and subsequently treated for 6 weeks with either a normal diet (ND) or a KD. Our electrophysiological and Western blot analyses assessed myocardial Ca2+ homeostasis in ventricular preparations in vivo. Unlike those on the KD, DM rats treated with an ND exhibited a prolonged QTc interval and action potential duration. Compared to the control and DM rats on the KD, DM rats treated with an ND also showed lower intracellular Ca2+ transients, sarcoplasmic reticular Ca2+ content, sodium (Na+)-Ca2+ exchanger currents (reverse mode), L-type Ca2+ contents, sarcoplasmic reticulum ATPase contents, Cav1.2 contents. Furthermore, these rats exhibited elevated ratios of phosphorylated to total proteins across multiple Ca2+ handling proteins, including ryanodine receptor 2 (RyR2) at serine 2808, phospholamban (PLB)-Ser16, and calmodulin-dependent protein kinase II (CaMKII). Additionally, DM rats treated with an ND demonstrated a higher frequency and incidence of Ca2+ leak, cytosolic reactive oxygen species, Na+/hydrogen-exchanger currents, and late Na+ currents than the control and DM rats on the KD. KD treatment may attenuate the effects of DM-dysregulated Na+ and Ca2+ homeostasis, contributing to its cardioprotection in DM.
Small and Medium-Sized Enterprises in the Digital Age: Understanding Characteristics and Essential Demands
Authors: Bradač Hojnik Barbara , Huđek Ivona
Published: November 2023

The article explores the implementation of digital technology in small and medium-sized Slovenian enterprises (SMEs), with a focus on understanding existing trends, obstacles, and necessary support measures during their digitalization progress. The surveyed companies mainly rely on conventional technologies like websites and teamwork platforms, emphasizing the significance of strong online communication and presence in the modern business world. The adoption of advanced technologies such as blockchain is limited due to the perceived complexity and relevance to specific sectors. This study uses variance analysis to identify potential differences in the digitalization challenges faced by companies of different sizes. The results indicate that small companies face different financial constraints and require more differentiated support mechanisms than their larger counterparts, with a particular focus on improving digital competencies among employees. Despite obtaining enhancements such as elevated operational standards and uninterrupted telecommuting via digitalization, companies still face challenges of differentiation and organizational culture change. The study emphasizes the importance of recognizing and addressing the different challenges and support needs of different-sized companies to promote comprehensive progress in digital transformation. Our findings provide important insights for policymakers, industry stakeholders, and SMEs to formulate comprehensive strategies and policies that effectively address the diverse needs and challenges of the digital transformation landscape.
Interoperability-Enhanced Knowledge Management in Law Enforcement: An Integrated Data-Driven Forensic Ontological Approach to Crime Scene Analysis
Authors: Spyropoulos Alexandros Z. , Bratsas Charalampos , Makris Georgios C. , Garoufallou Emmanouel , Tsiantos Vassilis
Published: November 2023

Nowadays, more and more sciences are involved in strengthening the work of law enforcement authorities. Scientific documentation is evidence highly respected by the courts in administering justice. As the involvement of science in solving crimes increases, so does human subjectivism, which often leads to wrong conclusions and, consequently, to bad judgments. From the above arises the need to create a single information system that will be fed with scientific evidence such as fingerprints, genetic material, digital data, forensic photographs, information from the forensic report, etc., and also investigative data such as information from witnesses’ statements, the apology of the accused, etc., from various crime scenes that will be able, through formal reasoning procedure, to conclude possible perpetrators. The present study examines a proposal for developing an information system that can be a basis for creating a forensic ontology—a semantic representation of the crime scene—through descriptive logic in the owl semantic language. The Interoperability-Enhanced information system to be developed could assist law enforcement authorities in solving crimes. At the same time, it would promote closer cooperation between academia, civil society, and state institutions by fostering a culture of engagement for the common good.
Pilot Testing of an Area-Wide Biological Control Strategy against the Coffee Berry Borer in Colombia Using African Parasitoids
Authors: Benavides Pablo , Gil Zulma Nancy , Escobar Luis Eduardo , Navarro-Escalante Lucio , Follett Peter , Diaz-Soltero Hilda
Published: November 2023

The coffee berry borer (CBB), Hypothenemus hampei (Ferrari, 1867) (Coleoptera: Curculionidae: Scolytinae), native to Africa, is a major global insect pest of coffee. It has invaded many coffee production areas around the world that do not have natural enemies. In this study, two African parasitoids, Prorops nasuta Waterston (Hymenoptera: Bethylidae) and Phymastichus coffea Waterston (Hymenoptera: Eulophidae), were mass-reared for field release against H. hampei in Chinchiná, Colombia. More than 1.5 million wasps of each species were released on a 61-hectare coffee farm in replicated plots, resulting in parasitism rates of up to 7.7% for P. nasuta and 56.3% for P. coffea. This led to a maximum reduction in H. hampei field populations of 81% from dispersal coffee plots (old coffee crops before plant stumping) and 64.3% in colonization coffee plots (new coffee crops with active growing and fruiting plants) within the farm. As a result of this area-wide strategy, the percentage of CBB-infested coffee berries in colonization coffee plots decreased from 51.1 to 77.5% compared to coffee plots without parasitoid releases. This approach offers a promising alternative to the use of chemical insecticides and could be integrated into current pest management programs to control H. hampei.
Investigation of Friction Hydro-Pillar Processing as a Repair Technique for Offshore Mooring Chain Links
Authors: dos Santos Rafael Eugenio , Chludzinski Mariane , Nunes Rafael Menezes , Marinho Ricardo Reppold , Paes Marcelo Torres Piza , Reguly Afonso
Published: November 2023

Repairing links of offshore mooring chains has presented a significant industry challenge, primarily arising from modifications in material properties, encompassing alterations in microstructure, hardness, and residual stress. In this context, the present work investigates the method of friction hydro-pillar processing (FHPP) applied to R4 grade mooring chain steel. Joints in as-repaired and post-weld heat treatment (PWHT) conditions were subjected to residual stress (RS) tests using the neutron diffraction technique, microhardness mapping, and microstructural evaluations. The process generated peaks of tensile and compressive stresses in different directions and hardness below that of the parent material in the softening zone. The friction zone promoted high hardness levels in the thermo-mechanically affected zone (TMAZ) with a maximum of 19% of the ultimate tensile strength of the parent material. As expected, the PWHT restored the RS and reduced the hardness; however, 4 h PWHT allowed the elimination of a hardness higher than that of the base material.
Genetic Diversity and Population Structure of Leptosphaeria biglobosa from the Winter Oilseed Rape Region in China
Authors: Zhou Kang , Zhang Jing , Yang Long , Li Guoqing , Wu Mingde
Published: November 2023

Phoma stem canker (blackleg), caused by the fungi Leptosphaeria maculans (anamorph Phoma lingam) and L. biglobosa, is one of the most devastating diseases in oilseed rape (Brassica napus L.) production worldwide. However, the population structure and genetic variation of L. biglobosa populations in China have rarely been investigated. Here, a collection of 214 fungal strains of blackleg from China (including Shaanxi, Jiangxi, Hubei, Jiangsu, Chongqing, Sichuan, Guangxi, Guizhou, Hunan, and Henan) and Europe (France and Ukraine) was identified as L. biglobosa. Three simple sequence repeat (SSR) markers were developed to characterize their population structure. The results showed that the Nei’s average gene diversity ranged from 0.6771 for the population from Jiangsu to 0.3009 for the population from Hunan. In addition, most of the genetic variability (96%) occurred within groups and there were only relatively small amounts among groups (4.0%) (FST = 0.043, p = 0.042 < 0.05). Pairwise population differentiation (FST) suggested that significant genetic differentiation was observed between different L. biglobosa populations. Bayesian and unweighted average method analysis revealed that these L. biglobosa strains were clustered into three branches, and three European strains were similar to those from eastern China. The pathogenicity assay showed that those in Group III were significantly more virulent than those in Group I (t = 2.69, p = 0.016). The study also showed that Group III was dominant in Chinese L. biglobosa populations, which provides new insights for the further study of population evolution and the management of this pathogen.
Compassion Satisfaction, Burnout, and Secondary Traumatic Stress among Respiratory Therapists in Mississippi: A Cross-Sectional Study
Authors: DeVaul Driscoll , Reulet Britney , Daniels Jacob , Zhu Xiaoqian , Wilkins Renee , Gordy Xiaoshan Z.
Published: November 2023

The COVID-19 pandemic had an immense effect on the well-being of healthcare professionals. In this study, researchers utilized a quantitative cross-sectional study design to investigate the degree of compassion satisfaction and fatigue amongst respiratory therapists in the state of Mississippi as a result of providing care to patients during the COVID-19 pandemic. Quantitative data were collected using an anonymous online survey that assessed the well-being and satisfaction of licensed respiratory therapists in the state of Mississippi. More specially, survey responses (n = 326) were quantitatively evaluated to measure the association between demographic variables and compassion satisfaction (CS), burnout (BO), and secondary traumatic stress (STS). Ninety-seven percent of participants reported a medium to high CS level, while 74% indicated having a medium to high level of BO, and 69% reported a medium to high level of STS. Neither age nor gender had a significant difference in CS (p = 0.504; p = 0.405), BO (p = 0.161; p = 0.285), or STS (p = 0.145; p = 0.252). Those working for more than 10 years at their current employer had higher CS (M = 38.7) and lower BO (M = 24.9) and STS (M = 24.8) scores. The number of hours worked, specifically overtime, had a significant impact on BO (β = 0.09, p = 0.028) and STS (β = 0.0.11, p = 0.019), but not CS (β = 0.02, p = 0.655). These findings suggest that the number of years employed in the field impacts the level of compassion satisfaction and contributes to lower levels of burnout and secondary traumatic stress. The age of a patient may also affect levels of compassion and burnout. The results of this study highlight the importance of developing incentive plans in an effort to retain employees.
Evolution of Radiation Fields from Involved Field to Involved Site—A Summary of the Current Guidelines by the International Lymphoma Radiation Oncology Group
Authors: Eich Hans Theodor , Pepper Niklas Benedikt , Oertel Michael
Published: November 2023

Radiation therapy has been proven to be highly effective in the treatment of lymphoma. With increasing rates of long-term survival, the reduction in toxicity has gained importance. The evolving understanding of the diseases’ biology, as well as technical and conceptual advances, allows for a precise and individualized application of irradiation. Smaller treatment fields and safety margins make it possible to spare healthy neighbouring tissue (organs at risk). The International Lymphoma Radiation Oncology Group (ILROG) has developed several guidelines to optimize radiotherapy treatment in lymphoma patients. Since its introduction in 2013, involved site radiotherapy (ISRT) has been adopted as the standard of care in most treatment regimens in adult lymphoma. This article serves as a summary of the current ILROG guidelines, also considering contemporary developments and possible future directions.
Review on Synthesis and Properties of Lithium Lanthanum Titanate
Authors: Okos Alexandru , Ciobota Cristina Florentina , Motoc Adrian Mihail , Piticescu Radu-Robert
Published: November 2023

The rapid development of portable electronic devices and the efforts to find alternatives to fossil fuels have triggered the rapid development of battery technology. The conventional lithium-ion batteries have reached a high degree of sophistication. However, improvements related to specific capacity, charge rate, safety and sustainability are still required. Solid state batteries try to answer these demands by replacing the organic electrolyte of the standard battery with a solid (crystalline, but also polymer and hybrid) electrolyte. One of the most promising solid electrolytes is Li3xLa2/3−xTiO3 (LLTO). The material nevertheless presents a set of key challenges that must be resolved before it can be used for commercial applications. This review discusses the synthesis methods, the crystallographic and the ionic conduction properties of LLTO and the main limitations encountered through a number of selected studies on this material.
Tailoring the Structure and Properties of Epitaxial Europium Tellurides on Si(100) through Substrate Temperature Control
Authors: Yu Fan , Qiu Xiaodong , Zhou Jinming , Huang Lin , Yang Bin , Liu Junming , Wu Di , Wang Gan , Zhang Yi
Published: November 2023

In this study, we improved the growth procedure of EuTe and realized the epitaxial growth of EuTe4. Our research demonstrated a selective growth of both EuTe and EuTe4 on Si(100) substrates using the molecular beam epitaxy (MBE) technique and reveals that the substrate temperature plays a crucial role in determining the structural phase of the grown films: EuTe can be obtained at a substrate temperature of 220 °C while lowering down the temperature to 205 °C leads to the formation of EuTe4. A comparative analysis of the transmittance spectra of these two films manifested that EuTe is a semiconductor, whereas EuTe4 exhibits charge density wave (CDW) behavior at room temperature. The magnetic measurements displayed the antiferromagnetic nature in EuTe and EuTe4, with Néel temperatures of 10.5 and 7.1 K, respectively. Our findings highlight the potential for controllable growth of EuTe and EuTe4 thin films, providing a platform for further exploration of magnetism and CDW phenomena in rare earth tellurides.
Simulating and Predicting the Mechanical Behavior of Electrospun Scaffolds for Cardiac Patches Fabrication
Authors: Gkouti Elli , Czekanski Aleksander , AlAttar Ahmed
Published: November 2023

Fabricating helical scaffolds using electrospinning is a common approach for cardiac implantation, aiming to achieve properties similar to native tissue. However, this process requires multiple experimental attempts to select suitable electrospun properties and validate resulting mechanical responses. To overcome the time and cost constraints associated with this iterative procedure, Finite Element Analysis (FEA) can be applied using stable hyperelastic and viscoelastic models that describe the response of electrospun scaffolds under different conditions. In this study, we aim to create accurate simulations of the viscoelastic behavior of electrospun helical scaffolds. We fabricated helical fibers from Poly (3-caprolactone) (PCL) using the electrospinning process to achieve this. The electrospun samples were subjected to uniaxial deformation, and their response was modelled using existing hyperelastic and stress relaxation models. The simulations were built on experimental data for specific deformation speed and maximum strain conditions. The FEM results were evaluated by accounting for the stress-softening phenomenon, which significantly impacted the models. The electrospun scaffolds’ predictions were performed in other than the initial experimental conditions to verify our simulations’ accuracy and reliability.
Experimental and Numerical Investigation on the Aerosol Micro-Jet 3D Printing of Flexible Electronic Devices
Authors: Zhang Yuanming , Zhu Tao , Jiao Junke , Song Shiyu , Wang Zhenqian , Wang Ziwen
Published: November 2023

In this study, the optimal forming parameters for printing flexible circuits using aerosol jet printing technology are explored through numerical simulation and experiments. The printhead during the deposition process is numerically simulated. By employing the controlled variable method, the process parameters such as gas flow rate, working distance, nozzle diameter, and printing speed are selected to investigate their effects on the morphology of the printed lines. Accordingly, single-factor experiments are designed to validate the printing of flexible circuits on both planar and curved substrates. Laser micro-sintering is utilized to improve the conductivity of the printed lines and ultimately fabricate flexible strain sensors. Under the sheath gas flow rate of 400 sccm, carrier gas flow rate of 100 sccm, working distance of 3 mm, nozzle diameter of 500 μm, and printing speed of 10 mm/s, the optimal morphology of the printed lines is achieved with low linewidth characteristics. The variations in the focal ratio, working distance, nozzle diameter, and printing speed significantly affect the minimum feature line width and morphology of the printed lines.
Thermal Regeneration of Spent Sand with Furfuryl Binder from an Ecological and Economic Point of View
Authors: Łucarz Mariusz , Dereń Michał
Published: November 2023

The recovery of the grain matrix from spent moulding sand is a constant challenge in making the best possible use of the deposits of quartz sand material, as well as in protecting them. In the case of spent sand with organic binders, the best method to recover the grain matrix is thermal regeneration. However, this method is expensive and requires adequate attention to the emission of harmful compounds into the atmosphere. This paper presents a new concept for implementing the thermal regeneration process. A suitable regeneration temperature was adopted for the furfuryl binder moulding sand, and a change in the design of the device was introduced in the area of the utilisation of gases generated during the combustion of the spent binder. To confirm the assumptions made, and to assess the appropriate suitability of the material recovered, the technological parameters of the material obtained were verified, namely, ignition losses, sieve analysis, bending strength, and pH value. The consumption of media for the process was also analysed from an economic point of view, as well as the emission of BTEX (a mixture of volatile aromatic hydrocarbons-benzene, toluene and three isomers of xylene) gases under different conditions of the process. On the basis of the research conducted, it was concluded that lowering the regeneration temperature of regeneration does not adversely affect the technological parameters of the moulding sand on the regenerate matrix. Changing the design of the regenerator does not result in increased emissions of harmful substances to the environment. Studies indicate that the appropriate setting of thermal regeneration parameters and the optimal design of the employed equipment are favourable factors in reducing the cost of the process while not compromising the quality of the moulding sand and the environmental impact.