bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunomodulatory and Anti-inflammatory Effects of Asiatic Acid in a DNCB-Induced Atopic Dermatitis Animal Model
Authors: Moon Gyo-Ha , Lee Yonghyeon , Kim Eun-Kyung , Chung Kang-Hyun , Lee Kwon-Jai , An Jeung-Hee
Published: July 2021

We examined the immunomodulatory and anti-inflammatory effects of asiatic acid (AA) in atopic dermatitis (AD). AA treatment (5–20 µg/mL) dose-dependently suppressed the tumor necrosis factor (TNF)-α level and interleukin (IL)-6 protein expression in interferon (IFN)-γ + TNF-α-treated HaCaT cells. The 2,4-dinitrocholrlbenzene (DNCB)-induced AD animal model was developed by administering two AA concentrations (30 and 75 mg/kg/d: AD + AA-L and AD + AA-H groups, respectively) for 18 days. Interestingly, AA treatment decreased AD skin lesions formation and affected other AD characteristics, such as increased ear thickness, lymph node and spleen size, dermal and epidermal thickness, collagen deposition, and mast cell infiltration in dorsal skin. In addition, in the DNCB-induced AD animal model, AA treatment downregulated the mRNA expression level of AD-related cytokines, such as Th1- (TNF-α and IL-1β and -12) and Th2 (IL-4, -5, -6, -13, and -31)-related cytokines as well as that of cyclooxygenase-2 and CXCL9. Moreover, in the AA treatment group, the protein level of inflammatory cytokines, including COX-2, IL-6, TNF-α, and IL-8, as well as the NF-κB and MAPK signaling pathways, were decreased. Overall, our study confirmed that AA administration inhibited AD skin lesion formation via enhancing immunomodulation and inhibiting inflammation. Thus, AA can be used as palliative medication for regulating AD symptoms.
Dehydroepiandrosterone Shifts Energy Metabolism to Increase Mitochondrial Biogenesis in Female Fertility with Advancing Age
Authors: Li Chia-Jung , Lin Li-Te , Tsui Kuan-Hao
Published: July 2021

Female reproductive aging is an irreversible process associated with a decrease in oocyte quality, which is a limiting factor for fertility. Previous studies have shown that dehydroepiandrosterone (DHEA) has been shown to improve in vitro fertilization (IVF) outcomes in older women. Herein, we showed that the decline in oocyte quality with age is accompanied by a significant decrease in the level of bioenergetic metabolism genes. We compared the clinical characteristics between groups of infertile women who either received DHEA or did not. Treatment with DHEA may enhance oocyte quality by improving energy production and metabolic reprogramming in cumulus cells (CCs) of aging women. Our results showed that compared with the group without DHEA, the group with DHEA produced a large number of day-three (D3) embryos, top-quality D3 embryos, and had improved ongoing pregnancy rate and clinical pregnancy rate. This may be because DHEA enhances the transport of oxidative phosphorylation and increases mitochondrial oxygen consumption in CCs, converting anaerobic to aerobic metabolism commonly used by aging cells to delay oocyte aging. In conclusion, our results suggest that the benefit of DHEA supplementation on IVF outcomes in aging cells is significant and that this effect may be mediated in part through the reprogramming of metabolic pathways and conversion of anaerobic to aerobic respiration.
Nutrition Strategy and Life Style in Polycystic Ovary Syndrome—Narrative Review
Authors: Szczuko Małgorzata , Kikut Justyna , Szczuko Urszula , Szydłowska Iwona , Nawrocka-Rutkowska Jolanta , Ziętek Maciej , Verbanac Donatella , Saso Luciano
Published: July 2021

Here we present an extensive narrative review of the broadly understood modifications to the lifestyles of women with polycystic ovary syndrome (PCOS). The PubMed database was analyzed, combining PCOS entries with causes, diseases, diet supplementation, lifestyle, physical activity, and use of herbs. The metabolic pathways leading to disturbances in lipid, carbohydrate, and hormonal metabolism in targeted patients are described. The article refers to sleep disorders, changes in mental health parameters, and causes of oxidative stress and inflammation. These conditions consistently lead to the occurrence of severe diseases in patients suffering from diabetes, the fatty degeneration of internal organs, infertility, atherosclerosis, cardiovascular diseases, dysbiosis, and cancer. The modification of lifestyles, diet patterns and proper selection of nutrients, pharmacological and natural supplementation in the form of herbs, and physical activity have been proposed. The progress and consequences of PCOS are largely modifiable and depend on the patient’s approach, although we have to take into account also the genetic determinants.
A Double-Blind, Randomized, Placebo-Controlled Trial to Evaluate the Efficacy of a Hydrolyzed Chicken Collagen Type II Supplement in Alleviating Joint Discomfort
Authors: Mohammed Anaam , He Siran
Published: July 2021

Joint pain and disease affects more than one in four adults in the United States. We conducted a double-blind, randomized, placebo-controlled trial to investigate the efficacy of a hydrolyzed chicken collagen type II (HCII) supplement in reducing joint-related discomfort such as pain and stiffness, and in improving mobility. We enrolled adults aged 40–65 (65.5% were women) who had joint discomfort, but had no co-morbidities, and who were not taking pain medications. The participants were randomized to receive either the HCII supplement (n = 47) or a placebo (n = 43) for eight weeks. At the baseline, and at week 4 and week 8, we administered the Western Ontario and McMaster Universities Arthritis Index (WOMAC) survey with three additional wrist-related questions and the Visual Analog Scale for assessments of joint-related symptoms. In the WOMAC stiffness and physical activity domains and in the overall WOMAC score, the HCII group had a significant reduction in joint-related discomforts compared with the placebo group. For example, at week 4, the HCII group had a 36.9% reduction in the overall WOMAC score, compared with a 14.3% reduction in the placebo group (p = 0.027). This HCII product is effective in reducing joint pain and stiffness and in improving joint function among otherwise healthy adults.
Exploring the Ability of Meningococcal Vaccines to Elicit Mucosal Immunity: Insights from Humans and Mice
Authors: Currie Elissa G. , Gray-Owen Scott D.
Published: July 2021

Neisseria meningitidis causes a devastating invasive disease but is also a normal colonizer of the human nasopharynx. Due to the rapid progression of disease, the best tool to protect individuals against meningococcal infections is immunization. Clinical experience with polysaccharide conjugate vaccines has revealed that an ideal meningococcal vaccine must prevent both invasive disease and nasal colonization, which confers herd immunity. However, not all meningococcal vaccines are equal in their ability to prevent nasal colonization, for unknown reasons. Herein, we describe recent efforts to utilize humanized mouse models to understand the impact of different meningococcal vaccines on nasal colonization. These mice are susceptible to nasal colonization, and they become immune following live nasal infection or immunization with matched capsule-conjugate or protein-based vaccines, replicating findings from human work. We bring together insights regarding meningococcal colonization and immunity from clinical work with findings using humanized mouse models, providing new perspective into the different determinants of mucosal versus systemic immunity. Then, we use this as a framework to help focus future studies toward understanding key mechanistic aspects left unresolved, including the bacterial factors required for colonization and immune evasion, determinants of nasal mucosal protection, and characteristics of an ideal meningococcal vaccine.
The Effect of Cyanine Dye NK-4 on Photoreceptor Degeneration in a Rat Model of Early-Stage Retinitis Pigmentosa
Authors: Liu Shihui , Matsuo Toshihiko , Miyaji Mary , Hosoya Osamu
Published: July 2021

The present study aimed to evaluate the effects of NK-4 on the apoptosis of photoreceptors in a rat model of retinitis pigmentosa and explore the mechanism underlying anti-apoptosis activity. The Royal College of Surgeons (RCS) rats received an intravitreous injection of NK-4 solution in the left eye and vehicle control in the right eye. Apoptosis was detected by TUNEL method in frozen sections of the eyes. The retinal tissues of the rats were dissected for RNA-seq analysis. Functional and pathway enrichment analyses of differentially expressed genes (DEGs) were performed by using Metascape and DAVID software. The expression levels of DEGs were confirmed by real-time quantitative PCR (RT-qPCR). The number of apoptotic cells decreased in the outer nuclear layer (ONL) and the thickness of the ONL was significantly thicker in the retina of NK-4-injected eyes, compared with control eyes. Five DEGs were identified by RNA-seq analysis, and Hmox1, Mt1, Atf5, Slc7a11, and Bdh2 were confirmed to be up-regulated by RT-qPCR. Functional and pathway enrichment analysis of the up-regulated genes showed that anti-apoptosis effects of NK-4 in the retina of RCS rats may be related to the pathways of metal ion homeostasis, negative regulation of neuron death, response to toxic substance, and pigment metabolic process. We found a potential mechanism of NK-4, providing a new viewpoint for the development of more therapeutic uses of NK-4 in the future.
A Self-Assembling Amphiphilic Peptide Dendrimer-Based Drug Delivery System for Cancer Therapy
Authors: Zhu Dandan , Zhang Huanle , Huang Yuanzheng , Lian Baoping , Ma Chi , Han Lili , Chen Yu , Wu Shengmei , Li Ning , Zhang Wenjie , Liu Xiaoxuan
Published: July 2021

Despite being a mainstay of clinical cancer treatment, chemotherapy is limited by its severe side effects and inherent or acquired drug resistance. Nanotechnology-based drug-delivery systems are widely expected to bring new hope for cancer therapy. These systems exploit the ability of nanomaterials to accumulate and deliver anticancer drugs at the tumor site via the enhanced permeability and retention effect. Here, we established a novel drug-delivery nanosystem based on amphiphilic peptide dendrimers (AmPDs) composed of a hydrophobic alkyl chain and a hydrophilic polylysine dendron with different generations (AmPD KK2 and AmPD KK2K4). These AmPDs assembled into nanoassemblies for efficient encapsulation of the anti-cancer drug doxorubicin (DOX). The AmPDs/DOX nanoformulations improved the intracellular uptake and accumulation of DOX in drug-resistant breast cancer cells and increased permeation in 3D multicellular tumor spheroids in comparison with free DOX. Thus, they exerted effective anticancer activity while circumventing drug resistance in 2D and 3D breast cancer models. Interestingly, AmPD KK2 bearing a smaller peptide dendron encapsulated DOX to form more stable nanoparticles than AmPD KK2K4 bearing a larger peptide dendron, resulting in better cellular uptake, penetration, and anti-proliferative activity. This may be because AmPD KK2 maintains a better balance between hydrophobicity and hydrophilicity to achieve optimal self-assembly, thereby facilitating more stable drug encapsulation and efficient drug release. Together, our study provides a promising perspective on the design of the safe and efficient cancer drug-delivery nanosystems based on the self-assembling amphiphilic peptide dendrimer.
Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway of Phaseolus vulgaris and Glycine max
Authors: de Koning Ramon , Kiekens Raphaël , Toili Mary Esther Muyoka , Angenon Geert
Published: July 2021

Raffinose family oligosaccharides (RFO) play an important role in plants but are also considered to be antinutritional factors. A profound understanding of the galactinol and RFO biosynthetic gene families and the expression patterns of the individual genes is a prerequisite for the sustainable reduction of the RFO content in the seeds, without compromising normal plant development and functioning. In this paper, an overview of the annotation and genetic structure of all galactinol- and RFO biosynthesis genes is given for soybean and common bean. In common bean, three galactinol synthase genes, two raffinose synthase genes and one stachyose synthase gene were identified for the first time. To discover the expression patterns of these genes in different tissues, two expression atlases have been created through re-analysis of publicly available RNA-seq data. De novo expression analysis through an RNA-seq study during seed development of three varieties of common bean gave more insight into the expression patterns of these genes during the seed development. The results of the expression analysis suggest that different classes of galactinol- and RFO synthase genes have tissue-specific expression patterns in soybean and common bean. With the obtained knowledge, important galactinol- and RFO synthase genes that specifically play a key role in the accumulation of RFOs in the seeds are identified. These candidate genes may play a pivotal role in reducing the RFO content in the seeds of important legumes which could improve the nutritional quality of these beans and would solve the discomforts associated with their consumption.
Genetic and Molecular Control of Somatic Embryogenesis
Authors: Salaün Camille , Lepiniec Loïc , Dubreucq Bertrand
Published: July 2021

Somatic embryogenesis is a method of asexual reproduction that can occur naturally in various plant species and is widely used for clonal propagation, transformation and regeneration of different crops. Somatic embryogenesis shares some developmental and physiological similarities with zygotic embryogenesis as it involves common actors of hormonal, transcriptional, developmental and epigenetic controls. Here, we provide an overview of the main signaling pathways involved in the induction and regulation of somatic embryogenesis with a focus on the master regulators of seed development, LEAFY COTYLEDON 1 and 2, ABSCISIC ACID INSENSITIVE 3 and FUSCA 3 transcription factors whose precise role during both zygotic and somatic embryogenesis remains to be fully elucidated.
Different Sensitivity Levels of the Photosynthetic Apparatus in Zea mays L. and Sorghum bicolor L. under Salt Stress
Authors: Stefanov Martin A. , Rashkov Georgi D. , Yotsova Ekaterina K. , Borisova Preslava B. , Dobrikova Anelia G. , Apostolova Emilia L.
Published: July 2021

The impacts of different NaCl concentrations (0–250 mM) on the photosynthesis of new hybrid lines of maize (Zea mays L. Kerala) and sorghum (Sorghum bicolor L. Shamal) were investigated. Salt-induced changes in the functions of photosynthetic apparatus were assessed using chlorophyll a fluorescence (PAM and OJIP test) and P700 photooxidation. Greater differences between the studied species in response to salinization were observed at 150 mM and 200 mM NaCl. The data revealed the stronger influence of maize in comparison to sorghum on the amount of closed PSII centers (1-qp) and their efficiency (Φexc), as well as on the effective quantum yield of the photochemical energy conversion of PSII (ΦPSII). Changes in the effective antenna size of PSII (ABS/RC), the electron flux per active reaction center (REo/RC) and the electron transport flux further QA (ETo/RC) were also registered. These changes in primary PSII photochemistry influenced the electron transport rate (ETR) and photosynthetic rate (parameter RFd), with the impacts being stronger in maize than sorghum. Moreover, the lowering of the electron transport rate from QA to the PSI end electron acceptors (REo/RC) and the probability of their reduction (φRo) altered the PSI photochemical activity, which influenced photooxidation of P700 and its decay kinetics. The pigment content and stress markers of oxidative damage were also determined. The data revealed a better salt tolerance of sorghum than maize, associated with the structural alterations in the photosynthetic membranes and the stimulation of the cyclic electron flow around PSI at higher NaCl concentrations. The relationships between the decreased pigment content, increased levels of stress markers and different inhibition levels of the function of both photosystems are discussed.
Effects of the Simulated Enhancement of Precipitation on the Phenology of Nitraria tangutorum under Extremely Dry and Wet Years
Authors: Bao Fang , Xin Zhiming , Li Jiazhu , Liu Minghu , Cao Yanli , Lu Qi , Gao Ying , Wu Bo
Published: July 2021

Plant phenology is the most sensitive biological indicator that responds to climate change. Many climate models predict that extreme precipitation events will occur frequently in the arid areas of northwest China in the future, with an increase in the quantity and unpredictability of rain. Future changes in precipitation will inevitably have a profound impact on plant phenology in arid areas. A recent study has shown that after the simulated enhancement of precipitation, the end time of the leaf unfolding period of Nitraria tangutorum advanced, and the end time of leaf senescence was delayed. Under extreme climatic conditions, such as extremely dry or wet years, it is unclear whether the influence of the simulated enhancement of precipitation on the phenology of N. tangutorum remains stable. To solve this problem, this study systematically analyzed the effects of the simulated enhancement of precipitation on the start, end and duration of four phenological events of N. tangutorum, including leaf budding, leaf unfolding, leaf senescence and leaf fall under extremely dry and wet conditions. The aim of this study was to clarify the similarities and differences of the effects of the simulated enhancement of precipitation on the start, end and duration of each phenological period of N. tangutorum in an extremely dry and an extremely wet year to reveal the regulatory effect of extremely dry and excessive amounts of precipitation on the phenology of N. tangutorum. (1) After the simulated enhancement of precipitation, the start and end times of the spring phenology (leaf budding and leaf unfolding) of N. tangutorum advanced during an extremely dry and an extremely wet year, but the duration of phenology was shortened during an extremely wet year and prolonged during an extremely drought-stricken year. The amplitude of variation increased with the increase in simulated precipitation. (2) After the simulated enhancement of precipitation, the start and end times of the phenology (leaf senescence and leaf fall) of N. tangutorum during the autumn advanced in an extremely wet year but was delayed during an extremely dry year, and the duration of phenology was prolonged in both extremely dry and wet years. The amplitude of variation increased with the increase in simulated precipitation. (3) The regulation mechanism of extremely dry or wet years on the spring phenology of N. tangutorum lay in the different degree of influence on the start and end times of leaf budding and leaf unfolding. However, the regulation mechanism of extremely dry or wet years on the autumn phenology of N. tangutorum lay in different reasons. Water stress caused by excessive water forced N. tangutorum to start its leaf senescence early during an extremely wet year. In contrast, the alleviation of drought stress after watering during the senescence of N. tangutorum caused a delay in the autumn phenology during an extremely dry year.
The Cell Division Cycle of Euglena gracilis Indicates That the Level of Circadian Plasticity to the External Light Regime Changes in Prolonged-Stationary Cultures
Authors: Kato Shota , Nam Hong Gil
Published: July 2021

In unicellular photosynthetic organisms, circadian rhythm is tightly linked to gating of cell cycle progression, and is entrained by light signal. As several organisms obtain a fitness advantage when the external light/dark cycle matches their endogenous period, and aging alters circadian rhythms, senescence phenotypes of the microalga Euglena gracilis of different culture ages were characterized with respect to the cell division cycle. We report here the effects of prolonged-stationary-phase conditions on the cell division cycles of E. gracilis under non-24-h light/dark cycles (T-cycles). Under T-cycles, cells established from 1-month-old and 2-month-old cultures produced lower cell concentrations after cultivation in the fresh medium than cells from 1-week-old culture. This decrease was not due to higher concentrations of dead cells in the populations, suggesting that cells of different culture ages differ in their capacity for cell division. Cells from 1-week-old cultures had a shorter circadian period of their cell division cycle under shortened T-cycles than aged cells. When algae were transferred to free-running conditions after entrainment to shortened T-cycles, the young cells showed the peak growth rate at a time corresponding to the first subjective night, but the aged cells did not. This suggests that circadian rhythms are more plastic in younger E. gracilis cells.
ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L
Authors: Spinedi Nahuel , Storb Romina , Aranda Elisabet , Romani Facundo , Svriz Maya , Varela Santiago A. , Moreno Javier E. , Fracchia Sebastian , Cabrera Juan , Batista-García Ramón Alberto , Ponce de León Inés , Scervino J. Martín
Published: July 2021

Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl a and 38.4% of Chl b, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (Fv/Fm). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that M. polymorpha tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that M. polymorpha under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus.
Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency
Authors: Hosoguchi Tomoya , Uchiyama Yuna , Komazawa Hinata , Yahata Masaki , Shimokawa Takashi , Tominaga Akiyoshi
Published: July 2021

Gerbera in vitro shoots were irradiated using three types of ion beams with different line energy transfers (LETs) to investigate the effective LET and absorbed doses for mutagenesis. Furthermore, genomic mutation analyses were conducted on the obtained mutants. Survival rate analysis showed a lower lethal dose 50% (LD50) with ion beams with higher LETs. Trait/morphological mutations exhibited changes in the color and shape of petals and male sterility. Irradiation conditions with the highest growth change and trait/morphological mutation rates in each ion were C irradiation at 10 Gy, Ar irradiation at 5 Gy, and Fe irradiation at 5 Gy, with a range of absorbed dose of around LD50 to about 10 Gy lower. The highest trait/morphological mutation rate was 14.1% with Ar irradiation at 5 Gy, which was one of the criteria for ion beam irradiation of gerbera in vitro shoots. Furthermore, the genomic mutation in the flower color, petal shape, and male sterile mutants were confirmed by genotype analysis using Genotyping by Random Amplicon Sequencing-Direct technology. This is the first study to report the efficient production of gerbera mutants that could be analyzed. Our findings may lead to more efficient gerbera mutant production and analysis technology.
Aerosol Charging with a Piezoelectric Plasma Generator
Authors: Krasa Helmut , Schriefl Mario A. , Kupper Martin , Melischnig Alexander , Bergmann Alexander
Published: July 2021

A novel piezoelectric plasma generator developed by TDK Electronics GmbH & Co. OG, the CeraPlas®, was investigated for its feasibility as a charger for aerosol particles. The CeraPlas® charger was directly compared to a commercially available bipolar X-ray charger regarding its efficiency of charging atomized NaCl particles in a size range from 30 nm to 100 nm. First results show the ability of the CeraPlas® to perform bipolar aerosol charging with high reproducibility, and measurements of the charge distribution in the Nit product yielded about 1012 m−3 s for our experimental charging configuration. Unwanted generation of ozone was suppressed by a dedicated charging chamber and operation in N2 atmosphere.
Predictions of Moisture Diffusion Behavior of Cellulose-Fiber-Reinforced Plain Weave Epoxy Composites
Authors: Yu Hang , Yang Jie
Published: July 2021

Cellulose-fiber-reinforced plain weave composites absorb lots of water from humid environments because of their inherent susceptibility to moisture. Moisture absorption experiments with cellulose fiber plain weave composites have been reported by some researchers; however, few theoretical studies have been performed to date to predict their moisture diffusion behavior. In this paper, the moisture diffusion behavior of cellulose-fiber-reinforced plain weave composite is predicted using a novel superposition method considering its microweave pattern. The overall moisture uptake of the composite is treated as moisture absorption superposition of the fiber bundles part, resin part, undulated fiber bundles and resin-rich part in the unit cell. The moisture diffusion of the undulated fiber bundles and resin-rich part is more complicated than the other parts; thus, a solution for a unique three-phase diffusion problem is used to solve this special moisture diffusion issue. Both finite element analysis and experiments are carried out to validate the proposed approach, with the results showing that the predictions can effectively characterize the moisture diffusion behavior of cellulose-fiber-reinforced plain weave composites.
Preparation and Characterization of Dextran Coated Iron Oxide Nanoparticles Thin Layers
Authors: Predoi Gabriel , Ciobanu Carmen Steluta , Iconaru Simona Liliana , Predoi Daniela , Dreghici Dragana Biliana , Groza Andreea , Barbuceanu Florica , Cimpeanu Carmen , Badea Monica-Luminita , Barbuceanu Stefania-Felicia , Furnaris Ciprian Florin , Belu Cristian , Ghegoiu Liliana , Raita Mariana Stefania
Published: July 2021

In the present study, we report the synthesis of a dextran coated iron oxide nanoparticles (DIO-NPs) thin layer on glass substrate by an adapted method. The surface morphology of the obtained samples was analyzed by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), optical, and metallographic microscopies. In addition, the distribution of the chemical elements into the DIO-NPs thin layer was analyzed by Glow Discharge Optical Emission Spectrometry (GDOES). Furthermore, the chemical bonds formed between the dextran and iron oxide nanoparticles was investigated by Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the HepG2 viability incubated with the DIO-NPs layers was evaluated at different time intervals using MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The goal of this study was to obtain a DIO-NPs thin layer which could be used as a coating for medical devices such as microfluidic channel, microchips, and catheter. The results of the surface morphology investigations conducted on DIO-NPs thin layer suggests the presence of a continuous and homogeneous layer. In addition, the GDOES results indicate the presence of C, H, Fe, and O signal intensities characteristic to the DIO-NPs layers. The presence in the IR spectra of the Fe-CO metal carbonyl vibration bonds prove that the linkage between iron oxide nanoparticles and dextran take place through carbon–oxygen bonds. The cytotoxicity assays highlighted that HepG2 cells morphology did not show any noticeable modifications after being incubated with DIO-NPs layers. In addition, the MTT assay suggested that the DIO-NPs layers did not present any toxic effects towards HEpG2 cells.
PnBA-b-PNIPAM-b-PDMAEA Thermo-Responsive Triblock Terpolymers and Their Quaternized Analogs as Gene and Drug Delivery Vectors
Authors: Skandalis Athanasios , Selianitis Dimitrios , Pispas Stergios
Published: July 2021

In this work, the ability of thermo-responsive poly [butyl acrylate-b-N-isopropylacrylamide-b-2-(dimethylamino) ethyl acrylate] (PnBA-b-PNIPAM-b-PDMAEA) triblock terpolymer self-assemblies, as well as of their quaternized analogs (PnBA-b-PNIPAM-b-QPDMAEA), to form polyplexes with DNA through electrostatic interactions was examined. Terpolymer/DNA polyplexes were prepared in three different amine over phosphate group ratios (N/P), and linear DNA with a 2000 base pair length was used. In aqueous solutions, the terpolymers formed aggregates of micelles with mixed PNIPAM/(Q)PDMAEA coronas and PnBA cores. The PnBA-b-PNIPAM-b-PDMAEA terpolymers’ micellar aggregates were also examined as carriers for the model hydrophobic drug curcumin (CUR). The complexation ability of the terpolymer with DNA was studied by UV–Vis spectroscopy and fluorescence spectroscopy by investigating ethidium bromide quenching. Fluorescence was also used for the determination of the intrinsic fluorescence of the CUR-loaded micellar aggregates. The structural characteristics of the polyplexes and the CUR-loaded aggregates were investigated by dynamic and electrophoretic light scattering techniques. Polyplexes were found to structurally respond to changes in solution temperature and ionic strength, while the intrinsic fluorescence of encapsulated CUR was increased at temperatures above ambient.
Viscoelastic Behaviour of Flexible Thermoplastic Polyurethane Additively Manufactured Parts: Influence of Inner-Structure Design Factors
Authors: Pelayo Fernández , Blanco David , Fernández Pedro , González Javier , Beltrán Natalia
Published: July 2021

Material extrusion based additive manufacturing is used to make three dimensional parts by means of layer-upon-layer deposition. There is a growing variety of polymers that can be processed with material extrusion. Thermoplastic polyurethanes allow manufacturing flexible parts that can be used in soft robotics, wearables and flexible electronics applications. Moreover, these flexible materials also present a certain degree of viscoelasticity. One of the main drawbacks of material extrusion is that decisions related to specific manufacturing configurations, such as the inner-structure design, shall affect the final mechanical behaviour of the flexible part. In this study, the influence of inner-structure design factors upon the viscoelastic relaxation modulus, E(t), of polyurethane parts is firstly analysed. The obtained results indicate that wall thickness has a higher influence upon E(t) than other inner-design factors. Moreover, an inadequate combination of those factors could reduce E(t) to a small fraction of that expected for an equivalent moulded part. Next, a viscoelastic material model is proposed and implemented using finite element modelling. This model is based on a generalized Maxwell model and contemplates the inner-structure design. The results show the viability of this approach to model the mechanical behaviour of parts manufactured with material extrusion additive manufacturing.
Analysis of the Energy Flow in a Municipal Wastewater Treatment Plant Based on a Supercritical Water Oxidation Reactor Coupled to a Gas Turbine
Authors: Mato Fidel A. , Peña Mar , García-Rodríguez Yoana , Bermejo María-Dolores , Martín Ángel
Published: July 2021

Biological municipal wastewater treatments lead to high sludge generation and long retention times, and the possibilities for recovery of the energy content of the input waste stream are very limited due to the low operating temperature. As an alternative, we propose a sequence of exclusively physicochemical, non-biological stages that avoid sludge production, while producing high-grade energy outflows favoring recovery, all in shorter times. Ultrafiltration and evaporation units provide a front-end concentration block, while a supercritical water oxidation reactor serves as the main treatment unit. A new approach for energy recovery from the effluent of the reactor is proposed, based on its injection in a gas turbine, which presents advantages over simpler direct utilization methods from operational and efficiency points of view. A process layout and a numerical simulation to assess this proposal have been developed. Results show that the model process, characterized with proven operating parameters, found a range of feasible solutions to the treatment problem with similar energy costs, at a fast speed, without sludge production, while co-generating the municipality’s average electricity consumption.
Hydrochloric Acid Modification and Lead Removal Studies on Naturally Occurring Zeolites from Nevada, New Mexico, and Arizona
Authors: Huntley Garven M. , Luck Rudy L. , Mullins Michael E. , Newberry Nick K.
Published: July 2021

Four naturally occurring zeolites were examined to verify their assignments as chabazites AZLB-Ca and AZLB-Na (Bowie, Arizona) and clinoptilolites NM-Ca (Winston, New Mexico) and NV-Na (Ash Meadows, Nevada). Based on powder X-ray diffraction, NM-Ca was discovered to be mostly quartz with some clinoptilolite residues. Treatment with concentrated HCl (12.1 M) acid resulted in AZLB-Ca and AZLB-Na, the chabazite-like species, becoming amorphous, as confirmed by powder X-ray diffraction. In contrast, NM-Ca and NV-Na, which are clinoptilolite-like species, withstood boiling in concentrated HCl acid. This treatment removes calcium, magnesium, sodium, potassium, aluminum, and iron atoms or ions from the framework while leaving the silicon framework intact as confirmed via X-ray fluorescence and diffraction. SEM images on calcined and HCl treated NV-Na were obtained. BET surface area analysis confirmed an increase in surface area for the two zeolites after treatment, NM-Ca 20.0(1) to 111(4) m2/g and NV-Na 19.0(4) to 158(7) m2/g. 29Si and 27Al MAS NMR were performed on the natural and treated NV-Na zeolite, and the data for the natural NV-Na zeolite suggested a Si:Al ratio of 4.33 similar to that determined by X-ray fluorescence of 4.55. Removal of lead ions from solution decreased from the native NM-Ca, 0.27(14), NV-Na, 1.50(17) meq/g compared to the modified zeolites, 30 min HCl treated NM-Ca 0.06(9) and NV-Na, 0.41(23) meq/g, and also decreased upon K+ ion pretreatment in the HCl modified zeolites.
Bayesian Analysis for Cardiovascular Risk Factors in Ischemic Heart Disease
Authors: Ghosh Sarada , Samanta Guruprasad , De la Sen Manuel
Published: July 2021

Ischemic heart disease (or Coronary Artery Disease) is the most common cause of death in various countries, characterized by reduced blood supply to the heart. Statistical models make an impact in evaluating the risk factors that are responsible for mortality and morbidity during IHD (Ischemic heart disease). In general, geometric or Poisson distributions can underestimate the zero-count probability and hence make it difficult to identify significant effects of covariates for improving conditions of heart disease due to regional wall motion abnormalities. In this work, a flexible class of zero inflated models is introduced. A Bayesian estimation method is developed as an alternative to traditionally used maximum likelihood-based methods to analyze such data. Simulation studies show that the proposed method has a better small sample performance than the classical method, with tighter interval estimates and better coverage probabilities. Although the prevention of CAD has long been a focus of public health policy, clinical medicine, and biomedical scientific investigation, the prevalence of CAD remains high despite current strategies for prevention and treatment. Various comprehensive searches have been performed in the MEDLINE, HealthSTAR, and Global Health databases for providing insights into the effects of traditional and emerging risk factors of CAD. A real-life data set is illustrated for the proposed method using WinBUGS.
Susceptibility of Tribolium castaneum (Coleoptera: Tenebrionidae) to the Fumigation of Two Essential Satureja Oils: Optimization and Modeling
Authors: Ebadollahi Asgar , Taghinezhad Ebrahim , Setzer William N. , Chen Guangnan
Published: July 2021

Due to the numerous side effects of synthetic pesticides, including environmental pollution, threats to human health, harmful effects on non-target organisms and pest resistance, the use of alternative healthy, available and efficient agents in pest management strategies is necessary. In this paper, the susceptibility of the cosmopolitan, polyphagous, stored-product pest Tribolium castaneum (red flour beetle) to the fumigation of the essential oils of two important medicinal and food additive plants, Satureja hortensis and S. intermedia, was investigated. The insecticidal properties of the essential oils were modeled and optimized using response surface methodology. It was found that a maximum significant mortality of 94.72% and 92.97% could be achieved within 72 h with the applications of 55.15 µL/L of S. hortensis (with the linear model) and 58.82 µL/L of S. intermedia (with the quadratic model), respectively. There were insecticidal terpenes and phenylpropanoids in both essential oils, including thymol (50.8%), carvacrol (11.2%) and p-cymene (13.4%), in the S. intermedia and estragole (68.0%) and methyl eugenol (5.6%) in the S. hortensis. It was suggested that the essential oils of S. hortensis and S. intermedia could be offered as promising pesticidal agents against T. castaneum for further studies in the management of such pests instead of detrimental synthetic pesticides.
Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology
Authors: Zhang Shu , Ge Qi , Chen Liang , Chen Keping
Published: July 2021

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional Chinese medicinal and edible plants, playing roles in improving the cardiovascular system, lowering blood sugar, anti-inflammation, anti-oxidation, and so on. Studies on the hypoglycemic effects of Pueraria lobata were also frequently reported. To determine the active ingredients and related targets of Pueraria lobata for DM, 256 metabolites were identified by LC/MS non targeted metabonomics, and 19 active ingredients interacting with 51 DM-related targets were screened. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy.
Analysis and Design of a Standalone Electric Vehicle Charging Station Supplied by Photovoltaic Energy
Authors: Atawi Ibrahem E. , Hendawi Essam , Zaid Sherif A.
Published: July 2021

Nowadays, there is a great development in electric vehicle production and utilization. It has no pollution, high efficiency, low noise, and low maintenance. However, the charging stations, required to charge the electric vehicle batteries, impose high energy demand on the utility grid. One way to overcome the stress on the grid is the utilization of renewable energy sources such as photovoltaic energy. The utilization of standalone charging stations represents good support to the utility grid. Nevertheless, the electrical design of these systems has different techniques and is sometimes complex. This paper introduces a new simple analysis and design of a standalone charging station powered by photovoltaic energy. Simple closed-form design equations are derived, for all the system components. Case-study design calculations are presented for the proposed charging station. Then, the system is modeled and simulated using Matlab/Simulink platform. Furthermore, an experimental setup is built to verify the system physically. The experimental and simulation results of the proposed system are matched with the design calculations. The results show that the charging process of the electric vehicle battery is precisely steady for all the PV insolation disturbances. In addition, the charging/discharging of the energy storage battery responds perfectly to store and compensate for PV energy variations.
Zero Tolerance of Children’s Sexual Abuse from Interreligious Dialogue
Authors: Pulido Cristina M. , Vidu Ana , de Mello Roseli Rodrigues , Oliver Esther
Published: July 2021

Child sexual abuse is a social problem that concerns our societies. The sustainable development goals have highlighted the eradication of child sexual abuse as one of the highest-priority goals of this century. Breaking the silence within religious communities is an essential step going forward. Therefore, establishing a dialogue between people of different religions is crucial to achieving this goal. The purpose of this article is to explore whether there are current interreligious dialogue initiatives based on scientific recommendations to prevent child sexual abuse. The method used herein is a qualitative document analysis of the selected initiatives. The results indicate that interreligious dialogue initiatives include scientific recommendations in their prevention programs. Furthermore, these successful initiatives connect religious values and the need to support victims and to break their silence. Based on these results, it can be concluded that interreligious initiatives for child sexual abuse prevention programs based on scientific evidence are crucial in order to eradicate child sexual abuse.
The Freedom of Religious Institutions and Human Flourishing in India: A Present and Future Research Agenda
Authors: Shah Rebecca Supriya
Published: July 2021

In this paper, I explore how India’s complex regime of control and management of religious institutions and communities—ironically, particularly Hindu institutions—influences the capacity of these institutions to promote various dimensions of human flourishing and socio-economic uplift among the most marginalized. In addition, I provide an overview of India’s highly varied landscape when it comes to the freedom of religious institutions from state control, and in particular discuss how some minority religious institutions experience fewer government constraints on some aspects of their freedom to self-identify and self-govern, especially when compared to some majority institutions, such as Hindu temples. Although some minority institutions still face constraints on certain aspects of their operations, the freedom they have to manage their internal affairs can, at times, translate into greater agility and the ability to innovate and flourish in the context of 21st-century India.
Machine Learning Applied to Banking Supervision a Literature Review
Authors: Guerra Pedro , Castelli Mauro
Published: July 2021

Machine learning (ML) has revolutionised data analysis over the past decade. Like innumerous other industries heavily reliant on accurate information, banking supervision stands to benefit greatly from this technological advance. The objective of this review is to provide a comprehensive walk-through of how the most common ML techniques have been applied to risk assessment in banking, focusing on a supervisory perspective. We searched Google Scholar, Springer Link, and ScienceDirect databases for articles including the search terms “machine learning” and (“bank” or “banking” or “supervision”). No language, date, or Journal filter was applied. Papers were then screened and selected according to their relevance. The final article base consisted of 41 papers and 2 book chapters, 53% of which were published in the top quartile journals in their field. Results are presented in a timeline according to the publication date and categorised by time slots. Credit risk assessment and stress testing are highlighted topics as well as other risk perspectives, with some references to ML application surveys. The most relevant ML techniques encompass k-nearest neighbours (KNN), support vector machines (SVM), tree-based models, ensembles, boosting techniques, and artificial neural networks (ANN). Recent trends include developing early warning systems (EWS) for bankruptcy and refining stress testing. One limitation of this study is the paucity of contributions using supervisory data, which justifies the need for additional investigation in this field. However, there is increasing evidence that ML techniques can enhance data analysis and decision making in the banking industry.
The Wearable Robotic Forearm: Design and Predictive Control of a Collaborative Supernumerary Robot
Authors: Vatsal Vighnesh , Hoffman Guy
Published: July 2021

This article presents the design process of a supernumerary wearable robotic forearm (WRF), along with methods for stabilizing the robot’s end-effector using human motion prediction. The device acts as a lightweight “third arm” for the user, extending their reach during handovers and manipulation in close-range collaborative activities. It was developed iteratively, following a user-centered design process that included an online survey, contextual inquiry, and an in-person usability study. Simulations show that the WRF significantly enhances a wearer’s reachable workspace volume, while remaining within biomechanical ergonomic load limits during typical usage scenarios. While operating the device in such scenarios, the user introduces disturbances in its pose due to their body movements. We present two methods to overcome these disturbances: autoregressive (AR) time series and a recurrent neural network (RNN). These models were used for forecasting the wearer’s body movements to compensate for disturbances, with prediction horizons determined through linear system identification. The models were trained offline on a subset of the KIT Human Motion Database, and tested in five usage scenarios to keep the 3D pose of the WRF’s end-effector static. The addition of the predictive models reduced the end-effector position errors by up to 26% compared to direct feedback control.