bluuebunny/crossref_metadata_2025 huggingface dataset:
Microseismic Monitoring and Disaster Warning via Mining and Filling Processes of Residual Hazardous Ore Bodies
Authors: Zhou Zilong , Huang Yinghua , Zhao Congcong
Published: September 2024

The thick ore bodies in the Xianglushan tungsten mine have been irregularly mined, forming a super large, connected irregular goaf group and tall, isolated irregular pillars inside. At the same time, there is a production capacity task of recovering residual and dangerous ore bodies. This poses the potential for serious ground-pressure disasters, such as roof caving, pillar collapse, and large-scale goaf collapse during mining. Based on the actual needs of the site, we established a microseismic monitoring system. After analyzing the mining and filling processes and their relationships, and, combined with the distribution characteristics of microseismic multiple parameters, we constructed a ground-pressure disaster warning mode and mechanism. We analyzed the stability of the goaf, further formed a warning system, and achieved disaster warning. In response to the current situation of the difficulty of early warning of ground pressure in the Xianglushan tungsten mine, continuous on-site monitoring of existing goaves, point pillars, and strip pillars, as well as analysis of stress changes during dynamic mining and filling processes, we explored scientific and reasonable early warning mechanisms and models, understanding the relationship between the changes in microseismic parameters during dynamic mining and filling processes and ground pressure, studying and improving the reliability of underground microseismic monitoring and early warning, and achieved the internal connection between building early warning systems and the prevention of ground-pressure disasters. The results indicate that the mining and filling process of the ore body is the main factor in maintaining a stable and balanced distribution of underground ground pressure in mining engineering. Microseismic monitoring can invert the evolution of ground pressure and form a feedback system with ground-pressure warning, achieving mine safety management.
Optimizing Additive Manufacturable Structures with Computer Vision to Enhance Material Efficiency and Structural Stability
Authors: Al Ali Musaddiq , Shimoda Masatoshi , Naguib Marc
Published: September 2024

This study introduces an innovative technique that merges computer vision with topology optimization to advance additive manufacturing. Employing advanced photogrammetry software, we obtain high-resolution images of the design domain, which are then used to develop accurate 3D models through meticulous scanning procedures. These models are transformed into an STL file format and remeshed using an adaptive algorithm within COMSOL 5.3 Multiphysics, facilitated by a custom MATLAB 2023 application. This integration achieves the optimal mesh resolution and precision in analytical assessments. We applied this technique to the design of a concrete pillar for 3D printing, targeting a 75% reduction in volume to improve the material efficiency and structural stability—critical factors for extraterrestrial applications. The design, captured with a 360-degree camera array, guided the MATLAB-based topology optimization process. By combining MATLAB’s optimization algorithms with COMSOL’s meshing and finite element analysis tools, we investigated various material-efficient configurations. The findings reveal a substantial volume reduction, especially in the central region of the design, effectively optimizing material utilization while preserving structural integrity. The optimization algorithm exhibited a swift and stable convergence, reaching near-optimal solutions within approximately 20 iterations.
Force Fields, Quantum-Mechanical- and Molecular-Dynamics-Based Descriptors of Radiometal–Chelator Complexes
Authors: Öztürk Işılay , Gervasoni Silvia , Guccione Camilla , Bosin Andrea , Vargiu Attilio Vittorio , Ruggerone Paolo , Malloci Giuliano
Published: September 2024

Radiopharmaceuticals are currently a key tool in cancer diagnosis and therapy. Metal-based radiopharmaceuticals are characterized by a radiometal–chelator moiety linked to a bio-vector that binds the biological target (e.g., a protein overexpressed in a particular tumor). The right match between radiometal and chelator influences the stability of the complex and the drug’s efficacy. Therefore, the coupling of the radioactive element to the correct chelator requires consideration of several features of the radiometal, such as its oxidation state, ionic radius, and coordination geometry. In this work, we systematically investigated about 120 radiometal–chelator complexes taken from the Cambridge Structural Database. We considered 25 radiometals and about 30 chelators, featuring both cyclic and acyclic geometries. We used quantum mechanics methods at the density functional theoretical level to generate the general AMBER force field parameters and to perform 1 µs-long all-atom molecular dynamics simulations in explicit water solution. From these calculations, we extracted several key molecular descriptors accounting for both electronic- and dynamical-based properties. The whole workflow was carefully validated, and selected test-cases were investigated in detail. Molecular descriptors and force field parameters for the complexes considered in this study are made freely available, thus enabling their use in predictive models, molecular modelling, and molecular dynamics investigations of the interaction of compounds with macromolecular targets. Our work provides new insights in understanding the properties of radiometal–chelator complexes, with a direct impact for rational drug design of this important class of drugs.
Quantitative Study of Vitamin K in Plants by Pressurized Liquid Extraction and LC-MS/MS
Authors: Bryshten Iryna , Paprotny Łukasz , Olszowy-Tomczyk Małgorzata , Wianowska Dorota
Published: September 2024

The health-promoting properties of vitamin K stimulate the growing interest in this compound, which translates into the development of new analytical methodologies for its determination. New, more efficient methods of its isolation are sought, paying increasingly more attention to the methods within currently available extraction techniques that, owing to the optimization of the process, not only increase the extraction efficiency but are also economical and environmentally friendly. This article proposes a procedure for the extraction and analysis of one of the vitamin K vitamers, i.e., vitamin K1, using PLE and LC-MS/MS. It has been shown that the PLE technique can be optimized with a mathematical model—accelerating and reducing the costs of the extraction process—which, together with process automation, bodes well for industrial applications. The optimized process was used to extract vitamin K1 from various vegetables, showing very different contents of the test compound ranging from 1.22 to 114.30 µg/g dry weight for avocado and spinach, respectively. In addition, by showing the effect of water within the material subjected to extraction on the variable yield of vitamin K1, attention was drawn to the need to standardize the analytical methods used in assessing the quality of food products.
Profile and Different Approaches for Size Characterization of Microplastics in Drinking Water from the Lisbon Water Supply System
Authors: Cordeiro Rodrigo D. M. , Cardoso Vitor V. , Carneiro Rui N. , Almeida Cristina M. M.
Published: September 2024

Microplastics (MPs) contribute to the overall pollution of water sources, affecting not only aquatic ecosystems but also water for human consumption (WHC). Currently, there needs to be a global consensus on safe levels of microplastics in WHC, which will allow regulatory efforts and risk assessments to be carried out. Therefore, this study aims to characterize MP particles in WHC of the Lisbon water supply system (LWSS) and compare two approaches to quantify these particles (length and width of the particles, and the area equivalent diameter (AED) of the particles). The quantification of MP particles was made via micro-FTIR (Fourier Transform Infrared Spectroscopy) on transmission mode after water filtration on 5 µm silicon filters. Thirty-eight WHC samples from the LWSS showed MPs up to 836 MPs/L, with an average value of 196 MPs/L. The most representative polymer was polyethylene (PE, 77.2%). The other eight polymers were also quantified. The length and width of MPs ranged between 84 µm and 41 µm, respectively. The AED of MPs ranged between 24 µm and 405 µm. The MP dimensions of both approaches can differ significantly.
Structural Transformation of Metastable Two-Electron Superatom Au-Doped Cu-Rich Alloy Nanocluster
Authors: Silalahi Rhone P. Brocha , Kahlal Samia , Saillard Jean-Yves , Liu C. W.
Published: September 2024

The ability to fabricate bimetallic clusters with atomic precision offers promising prospects for elucidating the correlations between their structures and properties. Nevertheless, achieving precise control at the atomic level in the production of clusters, including the quantity of dopant, characteristic of ligands, charge state of precursors, and structural transformation, have remained a challenge. Herein, we report the synthesis, purification, and characterization of a new bimetallic hydride cluster, [AuCu11(H){S2P(OiPr)2}6(C≡CPh)3] (AuCu11H). The hydride position in AuCu11H was determined using DFT calculations. AuCu11H comprises a ligand-stabilized defective fcc Au@Cu11 cuboctahedron. AuCu11H is metastable and undergoes a spontaneous transformation through ligand exchange into the isostructural [AuCu11(Cl){S2P(OiPr)2}6(C≡CPh)3] (AuCu11Cl) and into the complete cuboctahedral [AuCu12{S2P(OiPr)2}6(C≡CPh)4]+ (AuCu12) through an increase in nuclearity. These structural transformations were tracked by NMR and mass spectrometry.
Extracts and Terpenoids from Stevia Species as Potential Anthelmintics for Neglected Tropical Diseases Caused by Cestode Parasites
Authors: Cevasco Contreras María del Pilar , Borgo Jimena , Celentano Ana María , Elso Orlando Germán , Bach Hernán , Catalán Cesar Atilio Nazareno , Bivona Augusto Ernesto , Vaca Hugo Rolando , Rosenzvit Mara Cecilia , Sülsen Valeria Patricia
Published: September 2024

Cestodes are etiological agents of neglected diseases such as echinococcosis and cysticercosis, which are major public health problems. Antiparasitic treatment relies on a small number of approved drugs, which are often only partially effective, poorly tolerated and require prolonged administration. Thus, the discovery of novel potential treatments is critical. The Stevia genus (Asteraceae) includes species that are recognized as a source of bioactive compounds, with many species associated with medicinal uses. In this study, the cestocidal activity of four South American Stevia species that previously showed antiprotozoal activity was analyzed using a motility assay on the laboratory cestode model, Mesocestoides vogae. The four Stevia extracts showed cestocidal activity, with S. alpina var. alpina as the most active. The sesquiterpene lactones estafietin and eupatoriopicrin were purified from S. alpina var. alpina and S. maimarensis, respectively, and tested on M. vogae. Estafietin showed cestocidal activity, inhibiting parasite viability in a dose-dependent manner, even from the first day of incubation. Consistent with the motility effects, the extract of S. alpina var. alpina and estafietin induced marked alterations in the morphology of the parasite. The results of this report show that Stevia species represent a source of new molecules with potential for the treatment of neglected tropical diseases caused by cestodes.
A Flexible and Optical Transparent Metasurface Absorber with Broadband RCS Reduction Characteristics
Authors: Hayat Babar , Zhang Jinling , Khan Adil , Abbas Syed Muzahir , Majeed Abdul , Al-Bawri Samir Salem
Published: September 2024

Metasurface absorbers (MSAs) are of significant importance in a wide range of applications, such as in the field of stealth technology. Nevertheless, conventional designs demonstrate limited flexible characteristics and a lack of transparency, hence constraining their suitability for certain radar stealth applications. This study introduces a novel MSA operating in the broad microwave range, which exhibits both optical transparency and flexibility. The structure consists of a flexible substrate made of polyvinyl chloride (PVC), along with a resistive film composed of indium tin oxide (ITO). The proposed structure exhibits the ability to effectively absorb over 90% of the energy carried by incident electromagnetic (EM) waves across the frequency range of 9.85–41.76 GHz within an angular range of 0° to 60°. In addition, to assess the efficacy of the absorption performance, an examination of the radar cross-section (RCS) characteristics is conducted. The results indicate a reduction of over 10 dB across the aforementioned broad frequency spectrum, regardless of the central angle.
Heterogeneous Morphologies and Hardness of Co-Sputtered Thin Films of Concentrated Cu-Mo-W Alloys
Authors: Wissuchek Forrest , Derby Benjamin K. , Misra Amit
Published: September 2024

Heterogeneous microstructures in Cu-Mo-W alloy thin films formed by magnetron co-sputtering immiscible elements with concentrated compositions are characterized using scanning transmission electron microscopy (STEM) and nanoindentation. In this work, we modified the phase separated structure of a Cu-Mo immiscible system by adding W, which impedes surface diffusion during film growth. The heterogeneous microstructures in the Cu-Mo-W ternary system exhibited bicontinuous matrices and agglomerates composed of Mo(W)-rich phase. This is unique, as these are the slower-diffusing species, contrasting past reports of binary Cu-Mo thin films that exhibited Cu-rich agglomerates. The bicontinuous matrices comprised of Cu-rich and Mo(W)-rich phases exhibited bilayer thicknesses of less than 5 nm. The hardness of these thin films measured using nanoindentation is reported and compared to similar multilayers and nanocomposites in binary systems.
Charge Transfer in n-FeO and p-α-Fe2O3 Nanoparticles for Efficient Hydrogen and Oxygen Evolution Reaction
Authors: Humayun Amir , Manivelan Nandapriya , Prabakar Kandasamy
Published: September 2024

This study aims to explore the n-FeO and p-α-Fe2O3 semiconductor nanoparticles in hydrogen (HER) and oxygen (OER) evolution reactions and a combined full cell electrocatalyst system to electrolyze the water. We have observed a distinct electrocatalytic performance for both HER and OER by tuning the interplay between iron oxidation states Fe2+ and Fe3+ and utilizing phase-transformed iron oxide nanoparticles (NPs). The Fe2+ rich n-FeO NPs exhibited superior HER performance compared to p-α-Fe2O3 and Fe(OH)x NPs, which is attributed to the enhancement in n-type semiconducting nature under HER potential, facilitating the electron transfer for the reduction in H+ ions. In contrast, p-α-Fe2O3 NPs demonstrated excellent OER activity. An H-cell constructed using n-FeO||p-α-Fe2O3 NPs as cathode and anode achieved a cell voltage of 1.87 V at a current density of 50 mA/cm2. The cell exhibited remarkable stability after 30 h of activation and maintained the high current density of 100 mA/cm2 for 80 h with a negligible increase in cell voltage. This work highlights the semiconducting properties of n-FeO and p-α-Fe2O3 for the electrochemical water splitting system using the band bending phenomenon under the applied potential.
Effect of Purified Resveratrol Butyrate Ester Monomers against Hypertension after Maternal High-Fructose Intake in Adult Offspring
Authors: Tain You-Lin , Hou Chih-Yao , Tzeng Hong-Tai , Lin Shu-Fen , Chang-Chien Guo-Ping , Lee Wei-Chia , Wu Kay L. H. , Yu Hong-Ren , Chan Julie Y. H. , Hsu Chien-Ning
Published: September 2024

Background: Offspring hypertension arising from adverse maternal conditions can be mitigated through dietary nutritional supplementation, including resveratrol. Previously, we identified derivatives of resveratrol butyrate ester (RBE), specifically 3,4′-di-O-butanoylresveratrol (ED2) and 3-O-butanoylresveratrol (ED4), demonstrating their superior antioxidant capabilities compared to RBE itself. This study sought to assess the protective impact of maternal supplementation with ED2 or ED4 on offspring hypertension in a rat model subjected to a high-fructose (HF) diet during pregnancy and lactation. Methods: Female Sprague–Dawley rats were distributed into distinct dietary groups throughout pregnancy and lactation: (1) standard chow; (2) HF diet (60%); (3) HF diet supplemented with ED2 (25 mg/L); and (4) HF diet supplemented with ED4 (25 mg/L). Male offspring were euthanized at the age of 12 weeks. Results: The maternal HF diet induced hypertension in the offspring, which was mitigated by perinatal supplementation with either ED2 or ED4. These protective effects were attributed to the antioxidant properties of ED2 and ED4, resulting in an increased availability of nitric oxide (NO). Additionally, supplementation with ED2 was connected to an increased abundance of Bifidobacterium and Clostridium genera, which was accompanied by a decrease in Angelakisella and Christensenella. On the other hand, ED4 supplementation shielded rat offspring from hypertension by elevating concentrations of short-chain fatty acids (SCFAs) and their receptors while reducing trimethylamine-N-oxide (TMAO) levels. Conclusions: These findings highlight the potential of purified RBE monomers, ED2 and ED4, as preventive measures against hypertension resulting from a maternal high-fructose diet. Further research is warranted to explore their clinical applications based on these promising results.
The Effect of Dietary Types on Gut Microbiota Composition and Development of Non-Communicable Diseases: A Narrative Review
Authors: Soldán Martin , Argalášová Ľubica , Hadvinová Lucia , Galileo Bonzel , Babjaková Jana
Published: September 2024

Introduction: The importance of diet in shaping the gut microbiota is well established and may help improve an individual’s overall health. Many other factors, such as genetics, age, exercise, antibiotic therapy, or tobacco use, also play a role in influencing gut microbiota. Aim: This narrative review summarizes how three distinct dietary types (plant-based, Mediterranean, and Western) affect the composition of gut microbiota and the development of non-communicable diseases (NCDs). Methods: A comprehensive literature search was conducted using the PubMed, Web of Science, and Scopus databases, focusing on the keywords “dietary pattern”, “gut microbiota” and “dysbiosis”. Results: Both plant-based and Mediterranean diets have been shown to promote the production of beneficial bacterial metabolites, such as short-chain fatty acids (SCFAs), while simultaneously lowering concentrations of trimethylamine-N-oxide (TMAO), a molecule associated with negative health outcomes. Additionally, they have a positive impact on microbial diversity and therefore are generally considered healthy dietary types. On the other hand, the Western diet is a typical example of an unhealthy nutritional approach leading to an overgrowth of pathogenic bacteria, where TMAO levels rise and SCFA production drops due to gut dysbiosis. Conclusion: The current scientific literature consistently highlights the superiority of plant-based and Mediterranean dietary types over the Western diet in promoting gut health and preventing NCDs. Understanding the influence of diet on gut microbiota modulation may pave the way for novel therapeutic strategies.
The Impact of Yoyo Dieting and Resistant Starch on Weight Loss and Gut Microbiome in C57Bl/6 Mice
Authors: Phuong-Nguyen Kate , O’Hely Martin , Kowalski Greg M. , McGee Sean L. , Aston-Mourney Kathryn , Connor Timothy , Mahmood Malik Q. , Rivera Leni R.
Published: September 2024

Cyclic weight loss and subsequent regain after dieting and non-dieting periods, a phenomenon termed yoyo dieting, places individuals at greater risk of metabolic complications and alters gut microbiome composition. Resistant starch (RS) improves gut health and systemic metabolism. This study aimed to investigate the effect of yoyo dieting and RS on the metabolism and gut microbiome. C57BL/6 mice were assigned to 6 diets for 20 weeks, including control, high fat (HF), yoyo (alternating HF and control diets every 5 weeks), control with RS, HF with RS, and yoyo with RS. Metabolic outcomes and microbiota profiling using 16S rRNA sequencing were examined. Yoyo dieting resulted in short–term weight loss, which led to improved liver health and insulin tolerance but also a greater rate of weight gain compared to continuous HF feeding, as well as a different microbiota profile that was in an intermediate configuration between the control and HF states. Mice fed HF and yoyo diets supplemented with RS gained less weight than those fed without RS. RS supplementation in yoyo mice appeared to shift the gut microbiota composition closer to the control state. In conclusion, yoyo dieting leads to obesity relapse, and increased RS intake reduces weight gain and might help prevent rapid weight regain via gut microbiome restoration.
A Multi-Institutional, Retrospective, Observational Study on Administration Status and Safety of In-Hospital Oral Selenium Preparation in Pediatric Patients Predominantly Suffering from Gastrointestinal Disease
Authors: Saito Jumpei , Suzuki Eiji , Kobayashi Keiko , Doi Keisuke , Miwa Yosuke , Ihara Setsuko , Nakai Kei , Akabane Miki
Published: September 2024

Objectives: Selenium deficiency in patients with gastrointestinal diseases treated with long-term central venous nutrition is a clinical problem. Only injectable selenium is approved in Japan, and oral selenium preparations are prepared in hospitals from reagents, but their efficacy and safety are unknown. Methods: We conducted a retrospective study investigating the relationship between selenium administration and oral selenium formulations and adverse events. Results: In this study, 239 selenium-treated cases and 220 selenium-untreated cases adjusted for patient background were selected as a reference group. The median (interquartile range, IQR) age was 1.3 (0.4–4.4) and 1.3 (0.3–4.5) years, respectively; gastrointestinal diseases were most common in 110 (46.0%) and 104 (47.3%) cases. The median (IQR) duration of treatment or observation with oral selenium was 446 (128–1157) and 414 (141–1064) days, respectively. The median (IQR) dose per body weight at the maintenance dose was 2.6 (1.7–3.9) μg/kg, and the median (IQR) serum selenium concentration at the maintenance dose was 8.5 (7.0–10.6) μg/mL within the upper tolerated dose limit and approximately the reference range. There was no difference in selenium dose, serum selenium concentration, or serum-selenium-concentration-to-dose ratio (C/D ratio) for adverse events. The incidence of adverse events was compared with that of patients not treated with selenium. Conclusions: An oral selenium preparation administered below the upper tolerated dose limit can be used effectively and safely in pediatric patients.
Mediterranean Diet Modulation of Neuroinflammation-Related Genes in Elderly Adults at High Cardiovascular Risk
Authors: Hernando-Redondo Javier , Malcampo Mireia , Pérez-Vega Karla Alejandra , Paz-Graniel Indira , Martínez-González Miguel Ángel , Corella Dolores , Estruch Ramón , Salas-Salvadó Jordi , Pintó Xavier , Arós Fernando , Bautista-Castaño Inmaculada , Romaguera Dora , Lapetra José , Ros Emilio , Cueto-Galán Raquel , Fitó Montserrat , Castañer Olga
Published: September 2024

Individuals with dementia and neurodegenerative diseases (NDDs) often suffer from cardiovascular diseases (CVDs). Neuroinflammation driven by conditions involved in CVDs is linked to disruptions in the central nervous system triggering immune reactions, perpetuating an “inflammatory-like” environment. The Mediterranean diet (MedDiet), known for its anti-inflammatory and antioxidant properties, has been proposed as a key factor to attenuate these risks. Blood nuclear cell samples were collected from 134 participants of the PREDIMED trial, which randomized participants to three diets: one supplemented with extra-virgin olive oil (MedDiet-EVOO), another with nuts (MedDiet-Nuts), and a low-fat control diet. These samples were analyzed at baseline and 12-month follow-up to assess the impact of these dietary interventions on gene expression markers. We first selected target genes by analyzing intersections between NDD and CVD associations. Significant gene expression changes from baseline to 12 months were observed in the participants allocated to the MedDiet-EVOO, particularly in CDKN2A, IFNG, NLRP3, PIK3CB, and TGFB2. Additionally, TGFB2 expression changed over time in the MedDiet-Nuts group. Comparative analyses showed significant differences in TGFB2 between MedDiet-EVOO and control, and in NAMPT between MedDiet-Nuts and control. Longitudinal models adjusted for different covariates also revealed significant effects for TGFB2 and NAMPT. In conclusion, our results suggest that one year of traditional MedDiet, especially MedDiet-EVOO, modulates gene expression associated with CVD risk and NDDs in older adults at high CV risk.
1α,25-Dihydroxyvitamin D Downregulates Adipocyte Impact on Breast Cancer Cell Migration and Adipokine Release
Authors: Yum Chaehyun , Andolino Chaylen , Larrick Brienna , Sheeley Madeline P. , Teegarden Dorothy
Published: September 2024

Background/Objectives: Excess adiposity is associated with a higher risk of breast cancer metastasis and mortality. Evidence suggests that dietary vitamin D inhibits breast cancer metastasis. However, the mechanistic link between vitamin D’s regulation of adipocyte metabolism and metastasis has not been previously investigated. Therefore, the purpose of these experiments was to examine the effect of the active form of vitamin D, 1α,25-dihydroxyvitamin D (1,25(OH)2D), on adipocyte release of bioactive compounds and whether the impact on adipocytes leads to inhibition of breast cancer cell migration, an important step of metastasis. Methods: Differentiated 3T3-L1 adipocytes were treated with 1,25(OH)2D for two days, followed by either harvesting the adipocytes or collecting adipocyte-conditioned media without 1,25(OH)2D. A transwell migration assay was conducted with vehicle- or 1,25(OH)2D-conditioned media. In order to explore the mechanism underlying effects on breast cancer metastatic capability, the mRNA expression of leptin, adiponectin, insulin-like growth factor (IGF-1), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) was measured in adipocytes following either vehicle or 1,25(OH)2D treatment. Results: Conditioned media from 1,25(OH)2D-treated adipocytes inhibited the migration of metastatic MDA-MB-231 breast cancer cells compared to conditioned media from vehicle-treated adipocytes. Treatment of adipocytes with 1,25(OH)2D decreased mRNA expression of leptin, adiponectin, IGF-1, IL-6, and MCP-1. Consistent with mRNA expression, concentrations of leptin, adiponectin, IGF-1, and IL-6 in adipocyte-conditioned media were decreased with 1,25(OH)2D treatment, although MCP-1 remained unchanged. Conclusions: In summary, these results suggest that 1,25(OH)2D alters adipocyte secretions to prevent breast cancer metastasis.
Supplementation with Folic Acid or 5-Methyltetrahydrofolate and Prevention of Neural Tube Defects: An Evidence-Based Narrative Review
Authors: Samaniego-Vaesken María de Lourdes , Morais-Moreno Carmen , Carretero-Krug Alejandra , Puga Ana María , Montero-Bravo Ana María , Partearroyo Teresa , Gregorio Varela-Moreiras
Published: September 2024

Background: Folic acid (FA), which in its chemical form is pteroylglutamic acid, is the fully oxidised, water-soluble, monoglutamic form of vitamin B9. This compound is part of the folate group but with higher bioavailability, and it is found in vitamin supplements and fortified foods and drugs. Folate metabolism is complex and associated with various metabolic pathways, all of which confer protection on the cell and allow its survival. Methods: We conducted a non-systematic search of articles published in English and Spanish including controlled trials, cohort studies, systematic re-views, and meta-analyses were included, as well as key studies in animal models related to pharmacokinetic studies. Search terms encompassed: “folic acid”, “folates”, “5-metyltetrahydrofolate”, “5-MTHF”, “neural tube defects”, “supplementation”, “fortification”, AND “homocysteine” Results: A crucial role demonstrated for FA is to help prevent neural tube defects (NTDs). However, more studies are definitely still needed to establish 5-MTHF as a safe and effective therapeutic approach comparable with FA. Moreover, there is a lack of clinical studies that evaluate the efficacy of 5-MTHF supplementation in the prevention of NTDs. The present evidence-based narrative review discusses differences between FA and 5-MTHF in terms of structure, metabolism, bioavailability, clinical efficacy, and safety. Conclusions: Despite the potential value of 5-MTHF as an alternative to FA, clinical studies would be urgently needed to support the efficacy, dosage, timing, and/or safety of its use as a supplement.
Effects of Coffee on Gut Microbiota and Bowel Functions in Health and Diseases: A Literature Review
Authors: Saygili Sena , Hegde Shrilakshmi , Shi Xuan-Zheng
Published: September 2024

Background and objectives: As one of the most popular beverages in the world, coffee has long been known to affect bowel functions such as motility, secretion, and absorption. Recent evidence obtained in human and animal studies suggests that coffee has modulating impacts on gut microbiota. We aim to present an overview of the specific effects of coffee on gut microbiota composition, diversity, and growth. We will also critically review the impacts of coffee on bowel functions in health and diseases and discuss whether gut microbiota play a role in the coffee-associated functional changes in the gastrointestinal tract. Methods: We searched the literature up to June 2024 through PubMed, Web of Science, and other sources using search terms such as coffee, caffeine, microbiota, gastrointestinal infection, motility, secretion, gut–brain axis, absorption, and medication interaction. Clinical research in patients and preclinical studies in rodent animals were included. Results: A majority of the studies found that moderate consumption of coffee (<4 cups a day) increased the relative abundance of beneficial bacterial phyla such as Firmicutes and Actinobacteria and decreased Bacteroidetes. Moderate coffee consumption also increased Bifidobacterium spp. and decreased the abundance of Enterobacteria. Coffee consumption is reported to increase gut microbiota diversity. Although the effects of coffee on bowel functions have been known for a long time, it is not until recently that we have recognized that some of the effects of coffee may be partly due to its impacts on microbiota. Conclusions: The current literature suggests that moderate coffee consumption has beneficial effects on oral and gut microbiota and motility function. However, excessive coffee intake (>5 cups a day) is implicated in reflux disorders, periodontal diseases, and progression of Crohn’s disease. Further research in the field is needed, as there are many conflicting results regarding the impacts of coffee in the gastrointestinal tract.
First Case Report of Mycotic Abdominal Aortic Aneurysm Caused by Campylobacter fetus in Serbia
Authors: Medic Deana , Devrnja Milica , Batinic Nikola , Milosevic Djordje , Colovic Popadic Aleksandra , Gusman Vera
Published: September 2024

Background: Due to its distinct vascular tropism, Campylobacter fetus is recognized as a significant cause of severe systemic infections, especially in immunocompromised individuals, while it is rarely reported as a cause of gastrointestinal infections. Methods: A rare case of mycotic abdominal aortic aneurysm associated with Campylobacter fetus detected on the aneurysm wall itself was described. Results: A 68-year-old male was admitted to the hospital due to severe abdominal pain. The patient was afebrile, hemodynamically stable with elevated C-reactive protein levels. A physical examination revealed a palpable, pulsatile, tender mass located in the periumbilical region. Ultrasonography and multi-slice computer tomography angiography (MSCTA) identified an infrarenal abdominal aortic aneurysm with a maximum diameter of 6.5 cm, showing suspicious signs of dissection. Aneurysmectomy with Dacron tube graft interposition was performed. Although the blood cultures remained negative, the culture of the aneurysmal wall grew Campylobacter fetus, enabling early diagnosis and targeted antibiotic therapy. The patient was treated with meropenem for two weeks, followed by amoxicillin-clavulanate for another two weeks after hospital discharge. Conclusions: Campylobacter fetus associated with abdominal aortic aneurysms represents a life-threatening condition, posing a significant challenge in vascular surgery. Due to the lack of clear guidelines on antibiotic susceptibility testing and the treatment of infections associated with this pathogen, enhanced surveillance of Campylobacter fetus is necessary in both human and veterinary medicine.
Evaluation of the Impact of Infusion Set Design on the Particulate Load Induced by Vancomycin–Piperacillin/Tazobactam Incompatibility
Authors: Négrier Laura , Décaudin Bertrand , Treizebré Anthony , Guilbert Marie , Odou Pascal , Martin Mena Anthony
Published: September 2024

Introduction: Drug incompatibilities are among the most common medication errors in intensive care units. A precipitate can form and block the catheter or cause an adverse event in the patient. Intensive care units have implemented various strategies for limiting the occurrence of these incompatibilities, which have already been studied in vitro under standardized conditions. The objective of the present in vitro study was to continue these assessments by determining the impact of the infusion line geometry and the drugs’ position in the infusion set-up on the prevention of vancomycin–piperacillin/tazobactam incompatibility. Methods: Infusion lines with a different common volume, a multilumen medical infusion device, a dilute vancomycin solution, and separate infusions of incompatible drugs were evaluated separately. The infusion line outlet was connected to a dynamic particle counter. Results: Reducing the common volume, using multilumen medical devices, or spacing out the two incompatible drugs on the infusion line did not prevent the occurrence of a significant particulate load. Only dilution of the vancomycin solution was associated with a significantly lower particulate load and the absence of drug incompatibility. Conclusions: Our results show that under specific conditions, it is possible to reduce particulate contamination considerably.
Structural Basis for Monoclonal Antibody Therapy for Transthyretin Amyloidosis
Authors: Chakrabartty Avi
Published: September 2024

The disease of transthyretin (TTR) amyloidosis (ATTR) has been known since the 1960s, and during the past 60 or so years, there has been a sustained period of steady discoveries that have led to the current model of ATTR pathogenesis. More recent research has achieved major advances in both diagnostics and therapeutics for ATTR, which are having a significant impact on ATTR patients today. Aiding these recent achievements has been the remarkable ability of cryo-electron microscopy (EM) to determine high-resolution structures of amyloid fibrils obtained from individual patients. Here, we will examine the cryo-EM structures of transthyretin amyloid fibrils to explore the structural basis of the two monoclonal antibody therapies for ATTR that are in clinical trials, ALXN-2220 and Coramitug, as well as to point out potential applications of this approach to other systemic amyloid diseases.
The Anti-Arthritic Potential of the Ethanolic Extract of Salvia Lachnostachys Benth. Leaves and Icetexane Dinor-Diterpenoid Fruticuline B
Authors: Balsalobre Natália de M. , dos Santos-Procopio Elisangela , Oliveira Cristhian S. , Neves Silvia C. , Verdan Maria H. , Silva-Filho Saulo E. , Oliveira Rodrigo J. , Stefanello Maria É. A. , Kassuya Cândida A. L.
Published: September 2024

The decoction of Salvia lachnostachys Benth. leaves is used in Brazilian folk medicine for anti-spasmodic, antipyretic, and anxiolytic purposes. Some of the biological effects of an S. lachnostachys extract have been shown to be anti-inflammatory, anti-cancer, and antidepressant effects. In addition, this medicinal plant produces several compounds including icetexane diterpenoids, such as fruticuline A and fruticuline B. The aim of the present work was to evaluate the anti-hyperalgesic and anti-inflammatory properties of fruticuline B (FRUT B) and the ethanolic extract obtained from the leaves of S. lachnostachys (EESL) in experimental mouse models. EESL (30, 100, and 300 mg/kg) and FRUT B (1 mg/kg) were evaluated in articular inflammation-induced models in Swiss mice. In articular inflammation induced by Zymosan, EESL (300 mg/kg) and FRUT B (1 mg/kg) significantly reduced mechanical hyperalgesia (83.17% inhibition for EESL and 81.19% for FRUT B); edema (68.75% reduction for EESL and 33.66% for FRUT B); leukocyte migration (81.3% for EESSL and 92.2% for FRUT B), and nitric oxide production (88.3% for EESL and 74.4% for FRUT B). The exposure to fruticuline B significantly inhibited the edema (51.5%), mechanical (88.12%) and cold hyperalgesia (80.8%), and myeloperoxidase (MPO) (63.4%) activity 24 h after CFA injection. In the pleurisy model, FRUT B reduced 89.1% of leukocyte migration and 50.3% in nitric oxide production. Four hours after carrageenan injection, FRUT B (1 mg/kg) diminished 89.11% of mechanical hyperalgesia, 65.8% of paw edema, and 82.12% of the response to cold hyperalgesia. In the MTT test, EESL and fruticuline B caused no cytotoxicity. The present study revealed, for the first time, the anti-arthritic and anti-nociceptive effects of FRUT B, pointing out the therapeutic potential of the species to control inflammation and nociception. Future studies are needed to evaluate other biological properties of fruticuline B and to better understand its mechanism of action.
A Patent-Pending Ointment Containing Extracts of Five Different Plants Showed Antinociceptive and Anti-Inflammatory Mechanisms in Preclinical Studies
Authors: Barragan-Galvez Juan Carlos , Gonzalez-Rivera Maria Leonor , Jiménez-Cruz Juan C. , Hernandez-Flores Araceli , de la Rosa Guadalupe , Lopez-Moreno Martha L. , Yañez-Barrientos Eunice , Romero-Hernández Michelle , Deveze-Alvarez Martha Alicia , Navarro-Santos Pedro , Acosta-Mata Claudia , Isiordia-Espinoza Mario Alberto , Alonso-Castro Angel Josabad
Published: September 2024

Background/Objectives: The antinociceptive and anti-inflammatory effects of a patent-pending ointment containing plant extracts from Eucalyptus globulus, Curcuma longa, Hamamelis virginiana, Echinacea purpurea, and Zingiber officinale were evaluated. Methods: Plant extracts were chemically characterized by gas chromatography–mass spectroscopy. The antinociceptive activity of the ointment was assessed using the hot plate, tail flick, and formalin tests, whereas the anti-inflammatory activity was measured using the acute and chronic TPA-induced ear edema tests. Mechanisms of action were evaluated using inhibitors from signaling pathways related to pain response and by using histological analysis and assessing the expression and activity of pro-inflammatory mediators. Results: The ointment showed antinociceptive and anti-inflammatory effects like those observed with diclofenac gel (1.16% v/v) and ketoprofen gel (2.5% v/v). The antinociceptive actions of the ointment are mediated by the possible participation of the opiodergic system and the nitric oxide pathway. The anti-inflammatory response was characterized by a decrease in myeloperoxidase (MPO) activity and by a reduction in ear swelling and monocyte infiltration in the acute inflammation model. In the chronic model, the mechanism of action relied on a decrease in pro-inflammatory mediators such as COX-2, IL-1β, TNF-α, and MPO. An in-silico study with myristic acid, one of the compounds identified in the ointment’s plant mixture, corroborated the in vivo results. Conclusions: The ointment showed antinociceptive activities mediated by the decrease in COX-2 and NO levels, and anti-inflammatory activity due to the reduction in IL-1β and TNFα levels, a reduction in MPO activity, and a decrease in NF-κB and COX-2 expression.
Functionalized PLGA-Based Nanoparticles with Anti-HSV-2 Human Monoclonal Antibody: A Proof of Concept for Early Diagnosis and Targeted Therapy
Authors: Mariotti Melinda , Giacon Noah , Lo Cascio Ettore , Cacaci Margherita , Picchietti Simona , Di Vito Maura , Sanguinetti Maurizio , Arcovito Alessandro , Bugli Francesca
Published: September 2024

Background: Functionalized nanoparticles (NPs) represent a cutting edge in innovative clinical approaches, allowing for the delivery of selected compounds with higher specificity in a wider time frame. They also hold promise for novel theranostic applications that integrate both diagnostic and therapeutic functions. Pathogens are continuously evolving to try to escape the strategies designed to treat them. Objectives: In this work, we describe the development of a biotechnological device, Nano-Immuno-Probes (NIPs), for early detection and infections treatment. Human Herpes Simplex Virus 2 was chosen as model pathogen. Methods: NIPs consist of PLGA-PEG-Sulfone polymeric NPs conjugated to recombinant Fab antibody fragments targeting the viral glycoprotein G2. NIPs synthesis involved multiple steps and was validated through several techniques. Results: DLS analysis indicated an expected size increase with a good polydispersity index. Z-average and z-potential values were measured for PLGA-PEG-Bis-Sulfone NPs (86.6 ± 10.9 nm; –0.7 ± 0.3 mV) and NIPs (151 ± 10.4 nm; −5.1 ± 1.9 mV). SPR assays confirmed NIPs’ specificity for the glycoprotein G2, with an apparent KD of 1.03 ± 0.61 µM. NIPs exhibited no cytotoxic effects on VERO cells at 24 and 48 h. Conclusions: This in vitro study showed that NIPs effectively target HSV-2, suggesting the potential use of these nanodevices to deliver both contrast agents as well as therapeutic compounds.
Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode
Authors: Yoshida Yuya , Aoki Manami , Nagase Kalin , Marubashi Koichi , Kojima Hiroyuki , Itakura Shoko , Komatsu Syuuhei , Sugibayashi Kenji , Todo Hiroaki
Published: September 2024

Objectives: Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. Methods: Green fluorescent protein (GFP)- and luciferase-encoding DNA were administrated, and GFP and luciferase were evaluated. Results: A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes were confirmed after repeated DEP at 20 V with 5 min intervals. Conclusions: This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective for DNA vaccine delivery into skin tissue.
Hunting for Monolayer Black Phosphorus with Photoluminescence Microscopy
Authors: Pan Chenghao , Ma Yixuan , Wan Quan , Yu Boyang , Huang Shenyang , Yan Hugen
Published: September 2024

Monolayer black phosphorus (BP) holds great promise for naturally hyperbolic polaritons and correlated states in rectangular moiré superlattices. However, preparing and identifying high-quality monolayer BP are challenging due to its instability and high transparency, which limits extensive studies. In this study, we developed a method for rapidly and nondestructively identifying monolayer BP and its crystal orientation simultaneously using modified photoluminescence (PL) microscopy. The optical contrast of monolayer BP has been significantly increased by at least twenty times compared to previous reports, making it visible even on a transparent substrate. The polarization dependence of optical contrast also allows for the in situ determination of crystal orientation. Our study facilitates the identification of monolayer BP, expediting more extensive research on and potential industrial applications of this material.
A Modified Current-Mode VCSEL Driver for Short-Range LiDAR Sensor Applications in 180 nm CMOS
Authors: Li Juntong , Chon Yeojin , Choi Shinhae , Park Sung-Min
Published: September 2024

This paper presents a modified current-mode vertical-cavity surface-emitting laser (VCSEL) driver as a transmitter for short-range light detection and ranging (LiDAR) sensors, where a stable bias generator is suggested with a regulated cascode current mirror circuit to provide the bias current of 1 mA with a trivial deviation of 5.4%, even at the worst-case process–voltage–temperature (PVT) variations. Also, a modified current-steering logic circuit is exploited with N-type MOSFET (NMOS) switches to deliver the modulation currents of 0.1~10 mApp to the VCSEL diode simultaneously, with no overshoot distortions. Post-layout simulations of the modified current-mode VCSEL driver (m-CMVD), using 180 nm CMOS technology, demonstrate very large and clean output pulses with significantly reduced signal distortions. Hereby, the VCSEL diode is transformed into an equivalent circuit with a 1.6 V DC voltage and a 50 Ω resistor for circuit simulations. The proposed m-CMVD consumes a maximum of 11 mW from a 3.3 V supply voltage and the chip core occupies an area of 0.196 mm2.
Second-Order Microring Filter with Large Free-Spectral-Range and Wavelength-Tunable-Range over 50 nm
Authors: Gu Jiamei , Zhang Shuojian , Shao Qiongchan , Li Mingyu , Ma Xiao , He Jian-Jun
Published: September 2024

The high-order microring filter has been proposed for a larger free spectral range (FSR) compared with the single microring filter; therefore, it has great potential to be used in wavelength division multiplexing (WDM) systems. In this article, we have designed and fabricated a second-order microring filter made up of two rings connected in series with two Ti thermal heaters deposited above them. The large FSR of 56.8 nm is obtained by decreasing the difference of the radii between the two series rings, achieving similar FSRs to that of higher-order filters but with a simpler and more compact design. The average electrical tuning efficiencies of the two heaters are 0.186 nm/mW and 0.207 nm/mW, and the center wavelength of the filter can be tuned over the entire FSR with an applied electrical power of less than 40 mW.