bluuebunny/crossref_metadata_2025 huggingface dataset:
Reduced Order Modeling Using Advection-Aware Autoencoders
Authors: Dutta Sourav , Rivera-Casillas Peter , Styles Brent , Farthing Matthew W.
Published: April 2022

Physical systems governed by advection-dominated partial differential equations (PDEs) are found in applications ranging from engineering design to weather forecasting. They are known to pose severe challenges to both projection-based and non-intrusive reduced order modeling, especially when linear subspace approximations are used. In this work, we develop an advection-aware (AA) autoencoder network that can address some of these limitations by learning efficient, physics-informed, nonlinear embeddings of the high-fidelity system snapshots. A fully non-intrusive reduced order model is developed by mapping the high-fidelity snapshots to a latent space defined by an AA autoencoder, followed by learning the latent space dynamics using a long-short-term memory (LSTM) network. This framework is also extended to parametric problems by explicitly incorporating parameter information into both the high-fidelity snapshots and the encoded latent space. Numerical results obtained with parametric linear and nonlinear advection problems indicate that the proposed framework can reproduce the dominant flow features even for unseen parameter values.
Can DtN and GenEO Coarse Spaces Be Sufficiently Robust for Heterogeneous Helmholtz Problems?
Authors: Bootland Niall , Dolean Victorita
Published: April 2022

Numerical solutions of heterogeneous Helmholtz problems present various computational challenges, with descriptive theory remaining out of reach for many popular approaches. Robustness and scalability are key for practical and reliable solvers in large-scale applications, especially for large wave number problems. In this work, we explore the use of a GenEO-type coarse space to build a two-level additive Schwarz method applicable to highly indefinite Helmholtz problems. Through a range of numerical tests on a 2D model problem, discretised by finite elements on pollution-free meshes, we observe robust convergence, iteration counts that do not increase with the wave number, and good scalability of our approach. We further provide results showing a favourable comparison with the DtN coarse space. Our numerical study shows promise that our solver methodology can be effective for challenging heterogeneous applications.
Challenges in Kinetic-Kinematic Driven Musculoskeletal Subject-Specific Infant Modeling
Authors: Lim Yeram , Chambers Tamara , Walck Christine , Siddicky Safeer , Mannen Erin , Huayamave Victor
Published: April 2022

Musculoskeletal computational models provide a non-invasive approach to investigate human movement biomechanics. These models could be particularly useful for pediatric applications where in vivo and in vitro biomechanical parameters are difficult or impossible to examine using physical experiments alone. The objective was to develop a novel musculoskeletal subject-specific infant model to investigate hip joint biomechanics during cyclic leg movements. Experimental motion-capture marker data of a supine-lying 2-month-old infant were placed on a generic GAIT 2392 OpenSim model. After scaling the model using body segment anthropometric measurements and joint center locations, inverse kinematics and dynamics were used to estimate hip ranges of motion and moments. For the left hip, a maximum moment of 0.975 Nm and a minimum joint moment of 0.031 Nm were estimated at 34.6° and 65.5° of flexion, respectively. For the right hip, a maximum moment of 0.906 Nm and a minimum joint moment of 0.265 Nm were estimated at 23.4° and 66.5° of flexion, respectively. Results showed agreement with reported values from the literature. Further model refinements and validations are needed to develop and establish a normative infant dataset, which will be particularly important when investigating the movement of infants with pathologies such as developmental dysplasia of the hip. This research represents the first step in the longitudinal development of a model that will critically contribute to our understanding of infant growth and development during the first year of life.
Lulworthinone: In Vitro Mode of Action Investigation of an Antibacterial Dimeric Naphthopyrone Isolated from a Marine Fungus
Authors: Juskewitz Eric , Mishchenko Ekaterina , Dubey Vishesh K. , Jenssen Marte , Jakubec Martin , Rainsford Philip , Isaksson Johan , Andersen Jeanette H. , Ericson Johanna U.
Published: April 2022

Treatment options for infections caused by antimicrobial-resistant bacteria are rendered ineffective, and drug alternatives are needed—either from new chemical classes or drugs with new modes of action. Historically, natural products have been important contributors to drug discovery. In a recent study, the dimeric naphthopyrone lulworthinone produced by an obligate marine fungus in the family Lulworthiaceae was discovered. The observed potent antibacterial activity against Gram-positive bacteria, including several clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, prompted this follow-up mode of action investigation. This paper aimed to characterize the antibacterial mode of action (MOA) of lulworthinone by combining in vitro assays, NMR experiments and microscopy. The results point to a MOA targeting the bacterial membrane, leading to improper cell division. Treatment with lulworthinone induced an upregulation of genes responding to cell envelope stress in Bacillus subtilis. Analysis of the membrane integrity and membrane potential indicated that lulworthinone targets the bacterial membrane without destroying it. This was supported by NMR experiments using artificial lipid bilayers. Fluorescence microscopy revealed that lulworthinone affects cell morphology and impedes the localization of the cell division protein FtsZ. Surface plasmon resonance and dynamic light scattering assays showed that this activity is linked with the compound‘s ability to form colloidal aggregates. Antibacterial agents acting at cell membranes are of special interest, as the development of bacterial resistance to such compounds is deemed more difficult to occur.
Preparation of Doxorubicin-Loaded Carboxymethyl-β-Cyclodextrin/Chitosan Nanoparticles with Antioxidant, Antitumor Activities and pH-Sensitive Release
Authors: Mi Yingqi , Zhang Jingjing , Tan Wenqiang , Miao Qin , Li Qing , Guo Zhanyong
Published: April 2022

In this study, chitosan nanoparticles (HF-CD NPs) were synthesized by an ionic gelation method using negatively charged carboxymethyl-β-cyclodextrin and positively charged 2-hydroxypropyltrimethyl ammonium chloride chitosan bearing folic acid. The surface morphology of HF-CD NPs was spherical or oval, and they possessed relatively small particle size (192 ± 8 nm) and positive zeta potential (+20 ± 2 mV). Meanwhile, doxorubicin (Dox) was selected as model drug to investigate the prepared nanoparticles’ potential to serve as a drug delivery carrier. The drug loading efficiency of drug-loaded nanoparticles (HF-Dox-CD NPs) was 31.25%. In vitro release profiles showed that Dox release of nanoparticles represented a pH-sensitive sustained and controlled release characteristic. At the same time, the antioxidant activity of nanoparticles was measured, and chitosan nanoparticles possessed good antioxidant activity and could inhibit the lipid peroxidation inside the cell and avoid material infection. Notably, CCK-8 assay testified that the nanoparticles were safe drug carriers and significantly enhanced the antitumor activity of Dox. The nanoparticles possessed good antioxidant activity, pH-sensitive sustained controlled release, enhanced antitumor activity, and could be expected to serve as a drug carrier in future with broad application prospects.
Ishophloroglucin A, Isolated from Ishige okamurae, Alleviates Dexamethasone-Induced Muscle Atrophy through Muscle Protein Metabolism In Vivo
Authors: Yang Hye-Won , Oh Seyeon , Chung Dong-Min , Seo Minyoung , Park Shin Jae , Jeon You-Jin , Byun Kyunghee , Ryu BoMi
Published: April 2022

The in vitro capacity of Ishige okamurae extract (IO) to improve impaired muscle function has been previously examined. However, the mechanism underlying IO-mediated muscle protein metabolism and the role of its component, Ishophloroglucin A (IPA), in mice with dexamethasone (Dexa)-induced muscle atrophy remains unknown. In the present study, we evaluated the effect of IO and IPA supplementation on Dexa-induced muscle atrophy by assessing muscle protein metabolism in gastrocnemius and soleus muscles of mice. IO and IPA supplementation improved the Dexa-induced decrease in muscle weight and width, leading to enhanced grip strength. In addition, IO and IPA supplementation regulated impaired protein synthesis (PI3K and Akt) or degradation (muscle-specific ubiquitin ligase muscle RING finger and atrogin-1) by modulating mRNA levels in gastrocnemius and soleus muscles. Additionally, IO and IPA upregulated mRNA levels associated with muscle growth activation (transient receptor potential vanilloid type 4 and adenosine A1 receptor) or inhibition (myostatin and sirtuin 1) in gastrocnemius and soleus muscle tissues of Dexa-induced mice. Collectively, these results suggest that IO and IO-derived IPA can regulate muscle growth through muscle protein metabolism in Dexa-induced muscle atrophy.
Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards
Authors: Nguyen Thi Hanh , Wang San-Lang , Nguyen Anh Dzung , Doan Manh Dung , Tran Thi Ngoc , Doan Chien Thang , Nguyen Van Bon
Published: April 2022

α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, Pseudomonas aeruginosa TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation. P. aeruginosa TUN03 exhibited the highest aAIs productivity (3100 U/mL) in the medium containing 1.5% SHP with an initial pH of 7–7.5, and fermentation was performed at 27.5 °C for two days. Further, aAI compounds were investigated for scaled-up production in a 14 L-bioreactor system. The results revealed a high yield (4200 U/mL) in a much shorter fermentation time (12 h) compared to fermentation in flasks. Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance. Its purity was analyzed by high-performance liquid chromatography. HPC demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug. Notably, HPC was determined as a new aAI. The docking study indicated that HPC inhibits α-amylase by binding to amino acid Arg421 at the biding site on enzyme α-amylase with good binding energy (−9.3 kcal/mol) and creating two linkages of H-acceptors.
Aqueous Extract of Sea Squirt (Halocynthia roretzi) with Potent Activity against Human Cancer Cells Acts Synergistically with Doxorubicin
Authors: Zhu Yuting , Han Shanhao , Li Jianhui , Gao Hongwei , Dong Bo
Published: April 2022

Marine ascidian is becoming one of the main sources of an antitumor drug that has shown high bioactivity and extensive application in cancer treatment. Halocynthia roretzi, an edible marine sea squirt, has been demonstrated to have various kinds of biological activities, such as anti-diabetic, anti-hypertension, and enhancing immunity. In this study, we reported that aqueous extracts from the edible parts of H. roretzi presented significantly inhibiting the efficiency on HepG-2 cell viability. The separate mixed compound exhibited strong effects of inhibitory proliferation and induced apoptosis via the generation of ROS along with the concurrent loss of mitochondrial membrane potential on tumor cells. Furthermore, we found that there existed a significantly synergistic effect of the ascidian-extracted compound mixture with the anti-cancer drug doxorubicin. In the presence of the extracts from H. roretzi, the dose of doxorubicin at the cellular level could be reduced by a half dose. The extracts were further divided by semipreparative-HPLC and the active ingredients were identified as a mixture of fatty amide, which was composed of hexadecanamide, stearamide, and erucamide by UHPLC-MS/MS. Our results suggest that the potential toxicity of ascidian H. roretzi in tumor cells, and the compounds extracted from H. roretzi could be potentially utilized on functional nutraceuticals or as an adjunct in combination with chemotherapy.
Post-Cholecystectomy Right Hepatic Artery Pseudoaneurysm Induced by Generalized Seizure: A Case Report
Authors: Tsai Yueh-Hsun , Chang Hao-Ming
Published: April 2022

Pseudoaneurysm is a rare complication of laparoscopic cholecystectomy (LC). In most cases, the patient presents with gastrointestinal bleeding or hemoperitoneum. Here, we present a case with a post-cholecystectomy right hepatic artery pseudoaneurysm (PSA) induced by a generalized seizure. A 39-year-old male was sent to the emergency room with a generalized seizure and a loss of consciousness for approximately 5 min. Diffuse abdominal pain was complained of after consciousness returned. The surgical history of LC 13 days prior was mentioned. Abdominal computer tomography (CT) revealed a lobulated fluid accumulation in the gallbladder fossa with prominent fatty stranding and suspected biloma formation. After admission for one week, sharp abdominal pain was observed. Abdominal CT angiography revealed a right hepatic artery pseudoaneurysm. Transcatheter arterial embolization was performed with a total of seven platinum coils. In conclusion, it is important for doctors to take pseudoaneurysm into consideration in the patient who presents with seizure attack after receiving LC. Late discovery of PSA when it is ruptured can lead to fatal conditions, such as severe hemoperitoneum.
Acute Kidney Injury and Hyponatremia in Ultra-Trail Racing: A Systematic Review
Authors: Lecina Miguel , Castellar-Otín Carlos , López-Laval Isaac , Carrasco Páez Luis , Pradas Francisco
Published: April 2022

Background and objectives: Ultra-trail races can cause episodes of acute kidney injury (AKI) and exercise-associated hyponatremia (EAH) in healthy subjects without previous renal pathology. This systematic review aims to review the incidence of these two syndromes together and separately taking into account the length and elevation of the ultra-trail race examined. Materials and Methods: A systematic review was conducted through electronic search in four electronic databases (PubMed, EBSCO, Web of Science and Alcorze). Results: A total of 1127 articles published between January 2006 and December 31, 2021 were included, 28 of which met the inclusion criteria. The studies were categorized according to the length and stages of the race in four categories: medium (42 to 69 km), long (70 to 99 km), extra (>100 km) and multi-stage if they included various stages. A total of 2950 runners (666 females and 2284 males) were extracted from 28 publications. The AKI incidence found was 42.04% (468 cases of 1113), and 195 of 2065 were diagnosed with EAH, accounting for 9.11%. The concurrence of both pathologies together reached 11.84% (27 individuals) from a total of 228 runners with AKI and EAH simultaneously analyzed. Sorted by race category, the AKI+EAH cases were distributed as follows: 18 of 27 in the extra (13.63% and n = 132), 4 in the large (5.79% and n = 69) and 5 in the medium category (18.15% and n = 27). Conclusions: According to these results, extra and medium races showed a similar incidence of AKI+EAH. These findings underline the importance of the duration and intensity of the race and may make them responsible for the etiology of these medical conditions. Due to their variable incidence, EAH and AKI are often underdiagnosed, leading to poorer prognosis, increased condition seriousness and hindered treatment. The results of this review urge participants, coaches and race organizers to take measures to improve the early diagnosis and urgent treatment of possible EAH and AKI cases.
Predictive Factors in the Appearance and Evolution of Squamous Cell Carcinomas of the Oral Cavity
Authors: Carp Alexandra , Nicolau Andrei , Moscalu Mihaela , Popescu Eugenia
Published: April 2022

Background: Oral squamous cell carcinoma (OSCC) registered an alarming fall in the average age of individuals diagnosed in the last decade. Objectives: The aim of our study is to assess the main risk factors for OSCC specific to Romania and to identify patients at risk for this pathology. The purpose is to implement in the future a screening and early diagnosis program for OSCC in our country. Materials and Methods: A ten-year case-control study was conducted on patients selected from “St. Spiridon” Hospital-Iaşi, Romania. The study contained 1780 individuals diagnosed with oral squamous cell carcinoma. Results: For the patients under 46 years old: APC = −2.8 percent (95% CI: −24.4 to −7.1; p = 0.0012), with the observed rate of 30.18 percent. The incidence increased in patients aged 46 to 49 years (APC = 9.6%; 95% CI: 6.7 to −10.4; p = 0.0081). For the age group 49 to 64 years old: APC = −2.4 percent (95% CI: −5.3 to −1.6, p = 0.1239). For the age group 64–74: APC = −4.6, (95% CI: 1.4 to 6.9, p = 0.0108). The incidence of incidents was lower in the age group 74–80 (p = 0.0025). For the age group 80–91: APC = 8.1 (95% CI: 6.4 to 14.2, p = 0.0024), with the incidence of cases: APC = 8.1 (95% CI: 6.4 to 14.2, p = 0.0024). Univariate analysis revealed a substantially higher risk of developing oral carcinoma in males (OR = 4.43; CI: 3.84 to 5.80). Age above 60, cigarette usage and alcohol abuse are significant risk factors for OSCC. Patients with lymph node dissemination, ulcero-vegetant form, stages II and IV, whose therapeutic approach consisted of radiotherapy and chemotherapy or radiotherapy only had a worse rate of survival at 24 months post-therapy. Conclusions: Our study highlights the increase in the incidence of OSCC in Romania during the research period, the decrease in the average age of diagnosed patients, as well as the degree to which the studied population is exposed to the main risk factors specific to this geographical area.
Indication Criteria for Total Hip Arthroplasty in Patients with Hip Osteoarthritis—Recommendations from a German Consensus Initiative
Authors: Lützner Cornelia , Deckert Stefanie , Günther Klaus-Peter , Postler Anne Elisabeth , Lützner Jörg , Schmitt Jochen , Limb David , Lange Toni
Published: April 2022

Background: Osteoarthritis of the hip (hip OA) is a leading cause of pain and disability in elderly people. If non-surgical therapies become ineffective, patients may consider total hip arthroplasty (THA). The biggest challenge in recommending a THA is identifying patients for whom the benefits of this procedure outweigh the potential risks. The aim of this initiative was to develop a clinical practice guideline with accompanying algorithm to guide consultations on THA, supported by a pocket-sized checklist. Methods: The initiative “Evidence- and consensus-based indication criteria for total hip replacement (EKIT-Hip)” used a stepwise approach, starting with an inauguration workshop, where a multidisciplinary German stakeholder panel from various scientific societies agreed on the working process. A Project Coordinating Group (PCG) was formed, and it performed a comprehensive systematic literature search of guidelines and systematic reviews related to the indication criteria for THA, as well as factors influencing outcomes. Based on best-available evidence, preliminary recommendations were formulated by the PCG and discussed with the stakeholder panel during a consensus meeting. In addition, the panel was asked to assess the feasibility of an extracted algorithm and to approve a final checklist. Results: In total, 31 recommendations were approved by 29 representatives of 23 societies. These were used to underpin an algorithm (EKIT-Algorithm), which indicates the minimum requirements for a THA (confirmed diagnosis of hip OA, present and documented individual burden of illness, ineffectiveness of non-surgical therapies, and absence of any contraindications). Once these criteria are fulfilled, further considerations should encompass the medical implications of modifiable risk factors and patients’ individual treatment goals, as discussed during shared decision making. The subsequently developed checklist (EKIT-Checklist) lists relevant criteria for decision making. Conclusions: Adherence to the EKIT-Algorithm, conveniently accessed via the EKIT-Checklist, should improve the standardization of decision making leading to a recommendation for THA. By applying minimum requirements and patient-related risk factors, as well as considering patients’ individual goals, it is possible to identify patients for whom the benefits of THA may exceed the potential risks.
The Role of Power Doppler Ultrasonography in Caudal Epidural Injection
Authors: Tsai Yueh-Hsun , Huang Guo-Shu , Tang Chi-Tun , Yang Fu-Chi , Hsu Yi-Chih
Published: April 2022

Background and Objectives: Although the ultrasound-guided technique is used in caudal epidural injections, severe complications can happen if ultrasound cannot identify the occurrence of intravascular injection. To determine intraepidural and intravascular injection during caudal epidural injections, we used power Doppler ultrasonography (PDU) when injecting medications into the epidural space. Materials and Methods: This is a retrospective study that enrolled a total of 277 patients with refractory low back pain or degenerative disc from January 2019 to December 2019. The injectate flow of caudal epidural injections was examined with the assistance of PDU and confirmed by fluoroscopy. Four flow patterns were identified by PDU in our study: the “Earthworm sign,” the “Patch sign,” the ”Tubular sign” and the “Absent flow sign.” The accuracy of PDU in identifying intraepidural and intravascular injections was determined by fluoroscopy images recorded during each injection. Results: We evaluated 277 patients (mean age, 68.6 ± 13.2 years; 106 men). The “Patch sign” showed a sensitivity of 88.76% and a specificity of 80% in predicting epidural injection without intravascular injection. The “Earthworm sign” demonstrated a sensitivity of 70% and a specificity of 100% in detecting intravascular injection. The “Tubular sign” showed a specificity of 100% and a sensitivity of 9.4% in predicting successful epidural injection. The absence of a flow signal showed a sensitivity of 1.87% and a specificity of 90% in predicting successful epidural injection. Conclusions: Ultrasound-guided caudal epidural injection can accurately determine intraepidural and intravascular injections with the assistance of PDU and is thus a good alternative technique to fluoroscopy-guided caudal epidural injection.
Reinforcement of Percutaneous Pedicle Screw Fixation with Hydroxyapatite Granules in Patients with Osteoporotic Spine: Biomechanical Performance and Clinical Outcomes
Authors: Kanno Haruo , Onoda Yoshito , Hashimoto Ko , Aizawa Toshimi , Ozawa Hiroshi
Published: April 2022

In percutaneous pedicle screw (PPS) fixation of the osteoporotic spine, rigid screw fixation obtaining strong stabilization is important for achieving successful treatment outcomes. However, in patients with severe osteoporosis, it is difficult to obtain PPS fixation with sufficient stability. PPS fixation has potential disadvantages with respect to maintaining secure stabilization in comparison to conventional pedicle screw fixation. In PPS fixation, bone grafting to achieve posterior spine fusion is generally not applicable and transverse connectors between the rods cannot be used to reinforce the fixation. Various augmentation methods, including additional hooks, sublaminar bands, and hydroxyapatite (HA) sticks, are available for conventional pedicle screw fixation. On the other hand, there has been no established augmentation method for PPS fixation. Recently, we developed a novel augmentation technique for PPS fixation using HA granules. This technique allows the percutaneous insertion of HA granules into the screw hole along the guidewire prior to insertion of the PPS. We have used this augmentation technique for PPS fixation in various spine surgeries in patients with osteoporosis. In our previous studies, biomechanical analyses demonstrated that PPS fixation was significantly enhanced by augmentation with HA granules in the osteoporotic lumbar spine. Furthermore, augmentation with HA granules was considered to decrease the incidence of screw loosening and implant failure following PPS fixation in patients with osteoporotic spine. In this article, we describe the surgical procedures of the augmentation method using HA granules and summarize our data from the biomechanical analysis of augmentation for PPS fixation. We also review the surgical outcomes of PPS fixation with augmentation using HA granules.
Efficiency of Hyaluronic Acid in Infrabony Defects: A Systematic Review of Human Clinical Trials
Authors: Onisor Florin , Bran Simion , Mester Alexandru , Voina-Tonea Andrada
Published: April 2022

Background and objectives: The aim of this systematic review was to assess the electronic literature about the benefits of using hyaluronic acid (HA) in the surgical periodontal treatment of infrabony defects. Materials and methods: This review was conducted under the PRISMA guidelines. The electronic search was conducted on PubMed, Scopus, Web of Science, and Cochrane databases until February 2022. The inclusion criteria consisted of human clinical trials that reported the use of HA in open-flap debridement (OFD) for infrabony defects. The assessment of risk of bias was performed using the Cochrane risk of bias tool. Statistical analysis was performed using Review Manager. Results: Overall, three RCTs were found eligible for the statistical analysis. Probing depth (PD) reduction and clinical attachment level (CAL) gain in the HA test group presented WMs of −1.11 mm (95% CI −2.38 to 0.16 mm; p = 0.09) and −1.38 mm (95% CI −2.26 to −0.49 mm; p = 0.002), respectively. However, the heterogeneity of the RCTs was high, and the risk of bias, in general, was low. Conclusions: The use of hyaluronic acid seems to have beneficial effects in periodontal surgery using OFD, in terms of PD and CAL. To draw a clear conclusion, more adapted and well-designed clinical trials are needed to assess the advantage of this product in comparison with other products.
Primary Fallopian Tube Carcinoma Presenting with a Massive Inguinal Tumor: A Case Report and Literature Review
Authors: Maeda Michihide , Hisa Tsuyoshi , Matsuzaki Shinya , Ohe Shuichi , Nagata Shigenori , Lee Misooja , Mabuchi Seiji , Kamiura Shoji
Published: April 2022

Primary fallopian tube carcinoma (PFTC) has characteristics similar to those of ovarian carcinoma. The typical course of PFTC metastasis includes peritoneal dissemination and pelvic and paraaortic lymph node metastasis, while inguinal lymph node metastasis is rare. Moreover, the initial presentation of PFTC with an inguinal tumor is extremely rare. A 77-year-old postmenopausal woman presented with a massive 12-cm inguinal subcutaneous tumor. After tumor resection, histopathological and immunohistochemical analysis showed that the tumor was a high-grade serous carcinoma of gynecological origin. Subsequent surgery for total hysterectomy with bilateral salpingo-oophorectomy revealed that the tumor developed in the fallopian tube. She received adjuvant chemotherapy with carboplatin and paclitaxel, followed by maintenance therapy with niraparib. There has been no recurrence or metastasis 9 months after the second surgery. We reviewed the literature for cases of PFTC and ovarian carcinoma that initially presented with an inguinal tumor. In compliance with the Preferred Reporting Items for Systematic Reviews guidelines, a systematic literature search was performed through 31 January 2022 using the PubMed and Google scholar databases and identified 14 cases. In half of them, it was difficult to identify the primary site using preoperative imaging modalities. Disease recurrence occurred in two cases; thus, the prognosis of this type of PFTC appears to be good.
Epidemiology and Clinical Characteristics of People with Confirmed SARS-CoV-2 Infection during the Early COVID-19 Pandemic in Saudi Arabia
Authors: Alhamlan Fatimah S. , Almaghrabi Reem S. , Devol Edward B. , Alotaibi Anwar B. , Alageel Saleh M. , Obeid Dalia A. , Alraddadi Basem M. , Althawadi Sahar I. , Mutabagani Maysoon S. , Al-Qahtani Ahmed A.
Published: April 2022

This study provides epidemiologic and clinical characteristics of 492 consecutive patients diagnosed with SARS-CoV-2 infection at King Faisal Specialist Hospital and Research Centre in Saudi Arabia between March and September 2020. Data were collected from electronic case reports. The cohort was 54% male, with 20.4% aged >60 years, 19.9% aged 31–40 years, and 17% aged 41–50 years. The median incubation period was 16 days, with upper and lower 95% quartiles of 27 and 10 days, respectively. Most patients (79.2%) were symptomatic. Variables significantly different between symptomatic and asymptomatic patients were age, blood oxygen saturation percentage, hemoglobin level, lymphocyte count, neutrophil to lymphocyte (NTL) ratio, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level. Asymptomatic patients were mostly younger, with lower body mass index and ALT and AST levels but higher lymphocyte counts, NTL ratio, and CD4, CD8, natural killer cell, IgG, and IgM levels. Factors associated with increased risk of mortality were age (>42 years) and comorbidities, particularly diabetes mellitus and hypertension. Patients who were not given an antiviral regimen were associated with better prognosis than patients who received an antiviral regimen (HR, 0.07; 95% CI, 0.011–0.25). These findings will help clinicians and policymakers adopt best management and treatment options for SARS-CoV-2 infection.
Polyamine Metabolism in Leishmania Parasites: A Promising Therapeutic Target
Authors: Carter Nicola S. , Kawasaki Yumena , Nahata Surbhi S. , Elikaee Samira , Rajab Sara , Salam Leena , Alabdulal Mohammed Y. , Broessel Kelli K. , Foroghi Forogh , Abbas Alyaa , Poormohamadian Reyhaneh , Roberts Sigrid C.
Published: April 2022

Parasites of the genus Leishmania cause a variety of devastating and often fatal diseases in humans and domestic animals worldwide. The need for new therapeutic strategies is urgent because no vaccine is available, and treatment options are limited due to a lack of specificity and the emergence of drug resistance. Polyamines are metabolites that play a central role in rapidly proliferating cells, and recent studies have highlighted their critical nature in Leishmania. Numerous studies using a variety of inhibitors as well as gene deletion mutants have elucidated the pathway and routes of transport, revealing unique aspects of polyamine metabolism in Leishmania parasites. These studies have also shed light on the significance of polyamines for parasite proliferation, infectivity, and host–parasite interactions. This comprehensive review article focuses on the main polyamine biosynthetic enzymes: ornithine decarboxylase, S-adenosylmethionine decarboxylase, and spermidine synthase, and it emphasizes recent discoveries that advance these enzymes as potential therapeutic targets against Leishmania parasites.
Construct α-FeOOH-Reduced Graphene Oxide Aerogel as a Carrier for Glucose Oxidase Electrode
Authors: Yao Yue , Hou Changyu , Zhang Xin
Published: April 2022

A promising α-FeOOH-reduced graphene oxide aerogel (FeOOH-GA) has been prepared for the assembly of an enzyme electrode. The α-FeOOH-reduced graphene oxide aerogel was characterized by X-ray powder diffraction, field emission scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Raman, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. The results reveal that graphene oxide is reduced by Fe2+ ion and α-FeOOH nanorods anchored on the reduced graphene oxide sheet through the Fe-O-C bond. Analyses using scanning electron microscopy and the Brunauer–Emmett–Teller method show that FeOOH-GA displays a various and interconnected pore structure. The FeOOH-GA was used as a support material on the glass carbon electrode (GCE) for glucose oxidase (GOD). Electrochemistry properties and bioelectrocatalytic activities of Nafion/GOD/FeOOH-GA/GCE were achieved from cyclic voltammetry and electrochemical impedance spectroscopy. The results show that Nafion/GOD/FeOOH-GA/GCE maintains outstanding catalytic activity and electrochemical properties. The FeOOH-GA could immobilize GOD through the hydrophobicity of the reduced graphene oxide and hydroxide radical of α-FeOOH. Appropriate α-FeOOH and diversified pore structure are beneficial for electron transfer, enzyme electrode storage, and interfacial electron transfer rate. All results indicated that the α-FeOOH-reduced graphene oxide aerogel as a carrier could effectively immobilize the tested enzyme.
RCCS Bioreactor-Based Modeled Microgravity Affects Gastric Cancer Cells and Improves the Chemotherapeutic Effect
Authors: Rembiałkowska Nina , Baczyńska Dagmara , Dubińska-Magiera Magda , Choromańska Anna , Bieżuńska-Kusiak Katarzyna , Gajewska-Naryniecka Agnieszka , Novickij Vitalij , Saczko Jolanta , Przystupski Dawid , Kulbacka Julita
Published: April 2022

(1) Background: The main purpose of the study was to determine whether altered gravity might alter cell viability, improve drug delivery and modulate the expression of drug resistance-related genes. (2) Methods: This study investigated the intracellular mechanisms activated by microgravity in human resistant and sensitive gastric cancer cells (EPG85-257 RDB) and (EPG85-257 P). We used a rotary cell culture system (RCCS) developed by NASA to expose cells to altered gravity. The antitumor potential of microgravity was simulated by the RCCS bioreactor, and its effectiveness was evaluated in sensitive cell lines compared to chemotherapy-resistant cells concerning drug-sensitive cancer cells. Microgravity with chemotherapy was estimated by the viability assay, cytoskeleton imaging, MDR (multidrug resistance) gene expression analysis, MTCO-1 (mitochondrially encoded cytochrome C oxidase I), and 8-OHdG immunocytochemical analysis. (3) Results: We found that altered gravity combined with doxorubicin was cytotoxic to cancer cells. Cells following simulated microgravity revealed decreased expression of genes related to drug resistance and increased DNA/RNA damage marker expression. Cytoskeleton evaluation demonstrated significant reorganization of F-actin fibers after exposure to changed gravity conditions. (4) Conclusions: Intracellular alterations caused by simulated microgravity can increase gastric cancer cells’ sensitivity to chemotherapy. We have obtained satisfactory results showing the correlation between altered gravity and MDR phenomena which seems promising in future therapeutic applications.
Investigation of Pretreatment of Textile Wastewater for Membrane Processes and Reuse for Washing Dyeing Machines
Authors: Ćurić Iva , Dolar Davor
Published: April 2022

The aim of this study was to investigate the best pretreatment of textile wastewater (TWW) for membrane separation processes and the previously unexplored reuse of treated TWW for washing dyeing machines. Sand filtration (SF), coagulation, coagulation/flocculation, and ultrafiltration (UF) with hollow fiber membrane (ZW1) were used for pretreatment. Pretreatment selection was based on turbidity, total organic carbon (TOC), and color. SF and ZW1 were found to be the best pretreatments. In addition, the SF and ZW1 effluents were subjected to the 5 (PT) and 50 (MW) kDa UF flat sheet membranes to test removal efficiency. ZW1-PT was better in terms of removal results and fouling. To reduce the use of drinking water for washing dyeing machines, the characteristics of ZW1-PT effluent were compared with drinking water from a textile factory. TWW treated with this hybrid process fulfils the purpose of reuse for washing dyeing machines and can be used in Galeb d.d., Croatia, or in any other textile factory, saving up to 26,000 m3 of drinking water per year. This contributes to both sustainable production and the conservation of water resources.
A Computer Simulation Study of Thermal and Mechanical Properties of Poly(Ionic Liquid)s
Authors: Shim Youngseon , Shim Munbo , Kim Dae Sin
Published: April 2022

Thermal and mechanical properties of poly(ionic liquid)s (PILs), an epoxidized ionic liquid-amine network, are studied via molecular dynamics simulations. The poly(ionic liquid)s are designed with two different ionic liquid monomers, 3-[2-(Oxiran-2-yl)ethyl]-1-{4-[(2-oxiran-2-yl)ethoxy]phenyl}imidazolium (EIM2) and 1-{4-[2-(Oxiran-2-yl)ethyl]phenyl}-3-{4-[2-(oxiran-2-yl)ethoxy]benzyl}imidazolium (EIM1), each of which is networked with tris(2-aminoethyl)amine, paired with different anions, bis(trifluoromethanesulfonyl)imide (TFSI−) and chloride (Cl−). We investigate how ionic liquid monomers with high ionic strength affect structures of the cross-linked polymer networks and their thermomechanical properties such as glass transition temperature (Tg) and elastic moduli, varying the degree of cross-linking. Strong electrostatic interactions between the cationic polymer backbone and anions build up their strong structures of which the strength depends on their molecular structures and anion size. As the anion size decreases from TFSI− to Cl−, both Tg and elastic moduli of the PIL increase due to stronger electrostatic interactions present between their ionic moieties, making it favorable for the PIL to organize with stronger bindings. Compared to the EIM2 monomer, the EIM1 monomers and TFSI− ions generate a PIL with higher Tg and elastic moduli. This attributes to the less flexible structure of the EIM1 monomer for the chain rotation, in which steric hindrance by ring moieties in the EIM1-based PIL enhances their structural rigidity. The π-π stacking structures between the rings are found to increase in EIM1-based PIL compared to the EIM2-based one, which becomes stronger with smaller Cl− ion rather than TFSI−. The effect of the degree of the cross-linking on thermal and mechanical properties is also examined. As the degree of cross-linking decreases from 100% to 60%, Tg also decreases by a factor of 10–20%, where the difference among the given PILs becomes decreased with a lower degree of cross-linking. Both the Young’s (E) and shear (G) moduli of all the PILs decrease with degree of cross-linking, which the reduction is more significant for the PIL generated with EIM2 monomers. Transport properties of anions in PILs are also studied. Anions are almost immobilized globally with very small structural fluctuations, in which Cl− presents lower diffusivity by a factor of ~2 compared to TFSI− due to their stronger binding to the cationic polymer backbone.
Preparation and Characterization of Cellulose Nanofibers from Banana Pseudostem by Acid Hydrolysis: Physico-Chemical and Thermal Properties
Authors: Merais Mohammad Sobri , Khairuddin Nozieana , Salehudin Mohd Harfiz , Mobin Siddique Md. Bazlul , Lepun Philip , Chuong Wong Sie
Published: April 2022

Cellulose is a biopolymer that may be derived from a variety of agricultural wastes such as rice husks, wheat straw, banana, and so on. Cellulose fibril that is reduced in size, often known as nanocellulose (NC), is a bio-based polymer with nanometer-scale widths with a variety of unique properties. The use of NC as a reinforcing material for nanocomposites has become a popular research issue. This research paper focuses on the production of banana pseudostem cellulose nanofiber. Nano-sized fiber was obtained from banana pseudostem through several processes, namely, grinding, sieving, pre-treatment, bleaching, and acid hydrolysis. The product yield was found to be 40.5% and 21.8% for Musa acuminata and Musa balbisiana, respectively, by the weight of the raw fiber. The reduction in weight was due to the removal of hemicellulose and lignin during processing. Transmission electron microscopy (TEM) analysis showed that the average fiber size decreased from 180 µm to 80.3 ± 21.3 nm. Finally, FTIR analysis showed that the fibers experienced chemical changes after the treatment processes.
A Methyl-Modified Silica Layer Supported on Porous Ceramic Membranes for the Enhanced Separation of Methyl Tert-Butyl Ether from Aqueous Solution
Authors: Xu Ligang , Wang Yali , Li Qunyan , Cui Suping , Tang Mingxue , Nie Zuoren , Wei Qi
Published: April 2022

As a kind of volatile organic compound (VOC), methyl tert-butyl ether (MTBE) is hazardous to human health and destructive to the environment if not handled properly. MTBE should be removed before the release of wastewater. The present work supported the methyl-modified silica layer (MSL) on porous α-Al2O3 ceramic membranes with methyltrimethoxysilane (MTMS) as a precursor and pre-synthesized mesoporous silica microspheres as dopants by the sol-gel reaction and dip-coating method. MTMS is an environmentally friendly agent compared to fluorinated alkylsilane. The MSL-supported Al2O3 ceramic membranes were used for MTBE/water separation by pervaporation. The NMR spectra revealed that MTMS evolves gradually from an oligomer to a highly cross-linked methyl-modified silica species. Methyl-modified silica species and pre-synthesized mesoporous silica microspheres combine into hydrophobic mesoporous MSL. MSL makes the α-Al2O3 ceramic membranes transfer from amphiphilic to hydrophobic and oleophilic. The MSL-supported α-Al2O3 ceramic membranes (MSL-10) exhibit an MTBE/water separation factor of 27.1 and a total flux of 0.448 kg m−2 h−1, which are considerably higher than those of previously reported membranes that are modified by other alkylsilanes via the post-grafting method. The mesopores within the MSL provide a pathway for the transport of MTBE molecules across the membranes. The presence of methyl groups on the external and inner surface is responsible for the favorable separation performance and the outstanding long-term stability of the MSL-supported porous α-Al2O3 ceramic membranes.
One-Step Fabrication of Porous Membrane-Based Scaffolds by Air-Water Interfacial Phase Separation: Opportunities for Engineered Tissues
Authors: Allijn Iris , du Preez Nikola , Tasior Małgorzata , Bansal Ruchi , Stamatialis Dimitrios
Published: April 2022

Common methods for fabricating membrane-based scaffolds for tissue engineering with (hydrophobic) polymers include thermal or liquid-phase inversion, sintering, particle leaching, electrospinning and stereolithography. However, these methods have limitations, such as low resolution and pore interconnectivity and may often require the application of high temperatures and/or toxic porogens, additives or solvents. In this work, we aim to overcome some of these limitations and propose a one-step method to produce large porous membrane-based scaffolds formed by air-water interfacial phase separation using water as a pore-forming agent and casting substrate. Here, we provide proof of concept using poly (trimethylene carbonate), a flexible and biocompatible hydrophobic polymer. Membrane-based scaffolds were prepared by dropwise addition of the polymer solution to water. Upon contact, rapid solvent–non-solvent phase separation took place on the air-water interface, after which the scaffold was cured by UV irradiation. We can tune and control the morphology of these scaffolds, including pore size and porosity, by changing various parameters, including polymer concentration, solvent type and temperature. Importantly, human hepatic stellate cells cultured on these membrane-based scaffolds remained viable and showed no signs of pro-inflammatory stress. These results indicate that the proposed air-water interfacial phase separation represents a versatile method for creating porous membrane-based scaffolds for tissue engineering applications.
Applications of Polymeric Membranes with Carbon Nanotubes: A Review
Authors: Nitodas Steve F. , Das Mrinaleni , Shah Raj
Published: April 2022

Nanomaterials have been commonly employed to enhance the performance of polymeric membrane materials that are used in several industrial applications. Carbon nanotubes (CNTs) have gained notable attention over the years for use in membrane technology due to their anti-biofouling properties, salt rejection capability, exceptional electrical conductivity, and mechanical properties. This paper aims to discuss some of the recent applications of CNTs in membrane technology and their effect on a larger scale. The paper reviews successful case studies of incorporation of CNTs in membranes and their impact on water purification, desalination, gas separations, and energy storage, in an effort to provide a better understanding of their capabilities. Regarding the future trends of this technology, this review emphasizes improving the large-scale production processes and addressing environmental and health-related hazards of CNTs during production and usage.
Alpha-Crystallin-Membrane Association Modulated by Phospholipid Acyl Chain Length and Degree of Unsaturation
Authors: Trossi-Torres Geraline , Timsina Raju , Mainali Laxman
Published: April 2022

α-crystallin-membrane association increases with age and cataracts, with the primary association site of α-crystallin being phospholipids. However, it is unclear if phospholipids’ acyl chain length and degree of unsaturation influence α-crystallin association. We used the electron paramagnetic resonance approach to investigate the association of α-crystallin with phosphatidylcholine (PC) membranes of different acyl chain lengths and degrees of unsaturation and with and without cholesterol (Chol). The association constant (Ka) of α-crystallin follows the trends, i.e., Ka (14:0–14:0 PC) > Ka (18:0–18:1 PC) > Ka (18:1–18:1 PC) ≈ Ka (16:0–20:4 PC) where the presence of Chol decreases Ka for all membranes. With an increase in α-crystallin concentration, the saturated and monounsaturated membranes rapidly become more immobilized near the headgroup regions than the polyunsaturated membranes. Our results directly correlate the mobility and order near the headgroup regions of the membrane with the Ka, with the less mobile and more ordered membrane having substantially higher Ka. Furthermore, our results show that the hydrophobicity near the headgroup regions of the membrane increases with the α-crystallin association, indicating that the α-crystallin-membrane association forms the hydrophobic barrier to the transport of polar and ionic molecules, supporting the barrier hypothesis in cataract development.