bluuebunny/crossref_metadata_2025 huggingface dataset:
The benefit of the bismuth add-on to the 2-week clarithromycin-based triple regimen for Helicobacter pylori eradication: a propensity score-matched retrospective study
Authors: Shin Da Wit , Cheung Dae Young , Song Ji Hee , Choi Kyungseok , Lim Jihye , Lee Han Hee , Kim Jin Il , Park Soo-Heon
Published: March 2023

Background

Bismuth salt is bacteriostatic and bactericidal against Helicobacter pylori (H. pylori). Little is known about the benefit of bismuth itself. Recently in Korea, government regulation changed to allow bismuth add-on to conventional triple eradication regimens. Study aimed the additional benefit of the bismuth add-on to the 2-week clarithromycin-based triple regimen for H. pylori eradication.

Methods

A single-centered retrospective review of electronic medical records was conducted in Seoul, Korea. Treatment-naïve H. pylori infected subjects treated with the clarithromycin-based triple regimen were consecutively enrolled. After propensity score matching, 118 subjects who were treated with rabeprazole 20 mg, amoxicillin 1 g, and clarithromycin 500 mg twice daily for 14 days (PAC) and matched 118 subjects with PAC plus bismuth subcitrate potassium 300 mg twice daily for 14 days (PACB) were included in the final analysis. The primary endpoint was the eradication success rates in each group.Article title: Kindly check and confirm the edit made in the article title.Yes, I agree with the article title. 

Results

The eradication success rates were 91.5% (86.4–96.6%) for PACB regimen and 81.4% (74.2–88.5%) for PAC in the intention-to-treat analysis, and 97.3% (94.2–100%) for PACB and 88.1% (81.9–94.3%) for PAC in the per-protocol analysis. The relative risk of eradication failure for PACB over PAC was calculated as 0.184 (0.0492–0688, p value = 0.005) in multiple regression logistic analysis. Compliance and adverse event incidence were not different between the two groups.Author names: Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 1 Given name: [Da Wit], Last name: [Shin]. Author 2 Given name: [Dae Young], Last name: [Cheung]. Author 3 Given name: [Ji Hee], Last name: [Song]. Author 4 Given name: [Fan Hee], Last name: [Lee]. Author 5 Given name: [Jin Il], Last name: [Kim]. Yes. I found the names presented are accurate and in the correct sequence. Author 1 Given name: [Da Wit], Last name: [Shin].Author 2 Given name: [Dae Young], Last name: [Cheung].Author 3 Given name: [Ji Hee], Last name: [Song].Author 6 Given name: [Han Hee], Last name: [Lee].Author 7 Given name: [Jin Il], Last name: [Kim].

Conclusion

The bismuth add-on to the 2-week clarithromycin-based triple regimen increased the eradication success rate.
PROTEOMAS: a workflow enabling harmonized proteomic meta-analysis and proteomic signature mapping
Authors: Bahl Aileen , Ibrahim Celine , Plate Kristina , Haase Andrea , Dengjel Jörn , Nymark Penny , Dumit Verónica I.
Published: March 2023

Toxicological evaluation of substances in regulation still often relies on animal experiments. Understanding the substances’ mode-of-action is crucial to develop alternative test strategies. Omics methods are promising tools to achieve this goal. Until now, most attention was focused on transcriptomics, while proteomics is not yet routinely applied in toxicology despite the large number of datasets available in public repositories. Exploiting the full potential of these datasets is hampered by differences in measurement procedures and follow-up data processing. Here we present the tool PROTEOMAS, which allows meta-analysis of proteomic data from public origin. The workflow was designed for analyzing proteomic studies in a harmonized way and to ensure transparency in the analysis of proteomic data for regulatory purposes. It agrees with the Omics Reporting Framework guidelines of the OECD with the intention to integrate proteomics to other omic methods in regulatory toxicology. The overarching aim is to contribute to the development of AOPs and to understand the mode of action of substances. To demonstrate the robustness and reliability of our workflow we compared our results to those of the original studies. As a case study, we performed a meta-analysis of 25 proteomic datasets to investigate the toxicological effects of nanomaterials at the lung level. PROTEOMAS is an important contribution to the development of alternative test strategies enabling robust meta-analysis of proteomic data. This workflow commits to the FAIR principles (Findable, Accessible, Interoperable and Reusable) of computational protocols.
Perceptions, attitudes, and practices of a Belgian teaching hospital's physicians, pharmacists, and nurses regarding antibiotic use and resistance: survey towards targeted actions for Antimicrobial Stewardship
Authors: Briquet Caroline , Khaouch Youssra , Yombi Jean Cyr
Published: March 2023

Objectives

This study aimed to identify barriers to the proper use of antibiotics by healthcare professionals and to help the hospital Antimicrobial Stewardship develop suitable actions for the staff.

Methods

In a Belgian teaching hospital, a survey was conducted among physicians, pharmacists, and nurses involved in antibiotherapy. Questions from the 2019 European Center for Disease Prevention and Control (ECDC) survey were analyzed based on components of the COM-B model (capabilities, opportunities, and motivations). First, collected data were reviewed with the Ethnos software to analyze the different COM-B model components. For statistical analyses, responses were grouped into three clear-cut answers in a Fisher’s exact test.

Results

Overall, 400 staff members were included. We found that our professions, combined, have a good perception of antibiotic resistance (97.8%). For capabilities, however, only 77.2% state that they have sufficient knowledge, with 91.3%, 71.5%, and 63.0% for physicians, nurses, and pharmacists, respectively. For opportunities (access to resources, information, and training), it is observed that 72.2% report having easy access to the guidelines they need to manage infections. In comparison, for 64.2% of the respondents, this information changed their opinion on the useless or inappropriate prescription, administration, and delivery of antibiotics. For 55.0%, this information has enabled them to change their practices. Finally, for motivations, 92.8% of respondents state that they know about the link between their practices and the emergence and spread of antibiotic resistance. However, only 65.0% of participants say they have a role in managing antibiotic resistance. We found that 5 out of 8 questions are significantly dependent on the profession: 2 inquiries related to capability, 1 to opportunity, and 2 to motivation.

Conclusion

We found that responses to the ECDC questionnaire are related to the profession. While some topics are universal/cross-functional, others must be explicitly tailored to each professional category. Information is useless if not accessible. Communication and provision of documents are thus paramount.
An exploration of the possible educational opportunities and the challenges at the intersection of the physical and digital worlds occupied by 10–14 year-old students
Authors: Bogiannidis Nikolaos , Southcott Jane , Gindidis Maria
Published: March 2023

In developed countries, digital technologies are disrupting every facet of students’ lives. It is no longer an option to turn off devices and disconnect from the outside world at the school gate. Educators are struggling with the number of technologies being introduced and how to safely and effectively integrate these tools in classrooms that have no boundaries. In an era where it seems that each child takes central place on the world stage; always connecting, learning, creating and sharing, it is timely to investigate the experiences of technology-rich 10–14 years old Australian students’ experiences with technology in everyday life, and reflect on the persisting challenges and the new possibilities being created by the myriad choices of technological tools and their uses. The purposive selection of the participants was based on the volunteers’ daily access to personal devices and frequent use of applications and various online platforms. This inductive qualitative study used concept mapping as a tool for conducting research and thematic analysis to identify and explore patterns in the data. Our findings reveal the formation of a fluid and hybrid digital rhizomatic non-hierarchical and multiplicitous network that allows students to connect, think, act, interact, access ideas and resources in ways that may assist educators in closing the gap between formal learning and informal learning whilst living in a rich technology world.
Measuring impact of New Mexico prekindergarten on standardized test scores and high school graduation using propensity score matching
Authors: Courtney Jon R. , Garcia Janelle Taylor , Rowberry Jacob , Eckberg Nathan , Dinces Sarah M. , Lobaugh Clayton S. , Tolman Ryan T.
Published: March 2023

The long-term impact of prekindergarten programs is an important consideration given the trend of dedicating more resources to these programs. However, long-term impact of prekindergarten programs is not well-understood and recent studies have shown preschool effectiveness can vary across states and programs. A state run prekindergarten program in New Mexico was examined using propensity score matching to minimize selection bias. The research revealed a number of long-term impacts corresponding with prekindergarten participation for the cohort including a 9.7 percentage point increase in high school graduation rates and improved reading and math proficiency at third, sixth, and eighth grades. Considerations for future research and challenges in implementing prekindergarten programs are discussed.
Co-creating an intervention to promote physical activity in adolescents with intellectual disabilities: lessons learned within the Move it, Move ID!-project
Authors: Maenhout Laura , Verloigne Maïté , Cairns Deborah , Cardon Greet , Crombez Geert , Melville Craig , Van Hove Geert , Compernolle Sofie
Published: March 2023

Background

Co-creation is a method to develop acceptable, contextually appropriate and potentially more effective interventions. Adolescents with intellectual disabilities (ID) seldomly participate in research and program development due to the assumption that they lack the capacity to understand and discuss the related topics.

Objective

This study describes reflections on a co-creation process with adolescents with ID from the point of view of the researchers in developing an intervention to increase physical activity. It was the aim to highlight elements that must be considered when implementing co-creation and consequently formulate important lessons learned.

Methods

Twenty-three adolescents (14–22 y) with mild to moderate ID participated in six co-creation sessions at their school. The objectives and working methods in each session are described. Inductive thematic analysis was conducted on the researchers' reflection forms, which were completed after each session.

Results

Seven main themes could be distinguished from the data: experiences related to assistance (i.e., teacher presence) during sessions, the importance of building rapport, co-decision making power, the impact of different group dynamics, the relevance of adapted questioning, the influence of co-creative working methods and required characteristics of a co-creation researcher.

Conclusion

Seven lessons learned were formulated when preparing and conducting co-creation with adolescents with ID. Innovative, concrete (non-abstract) and creative working methods are highly needed. Describing the entire process transparently could be a first step to turn co-creative research into an evidence-based methodology.
Effects of potassium bromate on Rattus norvegicus brain antioxidant markers, acetylcholinesterase activity, and DNA fragmentation: investigation of therapeutic effect of Allium cepa
Authors: Agu Solomon Tsekohol , Alabi Akintola Ahmed , Meshioye Deborah Oluwaseun , Gbadegesin Michael Adedapo , Odunola Oyeronke Adunni
Published: March 2023

Background

Allium cepais well-known for its antioxidant capabilities and contains potent antioxidant quercetin (3, 30, 4, 5, 7-pentahydroxyflavone). We investigated the therapeutic effects of aqueous extract ofAllium cepa(AEAC) that is quercetin-rich against potassium bromate (KBrO3)-induced oxidative damage in the brains of male Wistar rats using biochemical, immunohistochemical, and histological markers. For 90 days, 40 male Wistar rats were administered KBrO3, KBrO3 + AEAC, and/or quercetin on alternate days, or AEAC and quercetin alone.

Results

KBrO3 significantly (p > 0.05) suppressed and diminished antioxidant enzymes and acetylcholinesterase activities with notable decreased total protein levels. Additionally, oxidative stress biomarkers (MDA and NO), as well as DNA fragmentation, all increased significantly (p > 0.05). The immunohistochemical expression of P53, caspase 3, and COX2 protein also increased significantly in the cerebral cortex of the KBrO3-treated groups, but BCL-2 protein expression decreased significantly. Histological examination of brain tissues revealed patterns that corresponded to the enzyme markers. The effects of KBrO3 were all attenuated by the administration of AEAC and quercetin.

Conclusions

This research demonstrates the therapeutic effects ofAllium cepaon KBrO3-induced oxidative stress, and biochemical perturbation in the brain ofRattus norvegicus. Even though the exact mechanism of action ofAllium cepaat the molecular level cannot be completely deduced from the results presented above, it could possibly be due to a combination of its antioxidant, anti-inflammatory, and apoptotic mechanisms. Further studies are required to examine the molecular pathways responsible for these aforementioned therapeutic effects.
Sexual dimorphism in some psycho-neuro-endocrine parameters at human
Authors: Kozyavkina Nataliya , Popovych Igor , Popovych Dariya , Zukow Walery , Bombushkar Igor
Published: March 2023

Background. Despite the well-documented relationships between HRV, EEG and anxiety parameters, studies of their sexual dimorphism, judging by the results of the meta-analysis, have so far been conducted separately. The purpose of this study is to clarify sexual dimorphism in almost synchronously registered psycho-neuro-endocrine parameters. Materials and Methods. The object of observation were practically healthy volunteers: 30 females (30÷76 yr) and 31 males (24÷69 yr). In basal conditions we recorded the ongoing HRV and EEG, determined serum levels of testosterone, calcitonin, cortisol, aldosterone, and triiodothyronine, estimated the severity of the trait and reactive anxiety. After 4 or 7 days, repeated testing was performed. Results. Regardless of age, females differ significantly from males, except for drastically lower levels of testosterone and calcitonin by definition (but not cortisol, aldosterone and triiodothyronine), lower levels of HRV-markers of sympathetic tone (but not heart rate), reactive anxiety, and beta-rhythm asymmetry. On the other hand, trait anxiety, levels of HRV-markers of vagal tone, variability and amplitude of the beta-rhythm, and its power spectral density (PSD) in 12 loci (maximum differences in T6, F3, and T3 loci), amplitude of the theta-rhythm and its PSD in 16 loci (maximum differences in F3, C3, and T3 loci), PSD of the alpha-rhythm in T3, T6, F7, and T4 loci as well as entropy of PSD in F7 and F8 loci are significantly higher in females than in males. The listed parameters are determined by testosterone for 31,7%, and calcitonin for 26,3%. The method of discriminant analysis revealed 20 EEG and 6 HRV parameters as well as trait and reactive anxiety, based on the totality of which males can be retrospectively recognized (without testosterone and calcitonin) with an accuracy of 90,3%, and females with an accuracy of 88,3%. Conclusion. The revealed differences between the sexes were not influenced by at least two bioactive factors, that is, they are robust sex markers.
Hüseyin Sadettin Arel’in Türk makam müziğinde çok seslilik anlayışı: “kemençe beşlemesi” örneği
Authors: Özgelen Orkun Zafer , Çolakoğlu Sarı Gözde , Durmuş Sadık Uğraş
Published: March 2023

Hüseyin Sadettin Arel (1880-1955)’in Türk Makam Müziği (TMM) çalgıları için bestelediği ikileme, üçleme, dörtleme1 gibi eserleri ve özellikle organolojik bir düşünce doğrultusunda ele aldığı kemençe beşlemesi projesi, Batıdaki “çok seslilik” ve “çalgılama” anlayışının TMM çalgılarıyla gerçekleştirilebileceğinin ifadesidir. Arel, bu fikri Batı müziğindeki; quartet, quintet gibi yaylı çalgı topluluklarından almıştır. Bir yandan TMM’yi meşrulaştırmak amacı güden Arel, Batı müziğinin ses sistemi üzerine inşa ettiği bir ‘ses’ ve ‘eğitim’ sistemi sunmakta ve aynı öykünmeyi çok sesli çalgısal eserleriyle de vermeye çalışmaktadır. Makam müziği ve çok sesliliği bir arada kullandığı eserlerinde, bestecilere yeni anlayışı sunmayı hedefleyen Arel, yaşadığı dönemin müzik ve kültürel düşüncesini yansıtmaktadır. İki bölüme ayırabileceğimiz çalışmamızın birinci bölümünde, müzik çalgılarının sosyal ve kültürel hayattaki öneminin yanı sıra, ‘kemençe beşlemesi’ projesinin tarihsel kaynaklar doğrultusunda bir özeti verilmiş ve modernleşme anlayışının projeye katkısına kısaca değinilmiştir. Çalışmanın metodolojisi ve sınırlılıkları ortaya konmuş ve kullanılan terimlerin nedenselliği açıklanmıştır. Bu çerçevede beşlemede karşımıza çıkan müzikal terimler, eserin formu, donanım ve anahtar kullanımları gösterilmiştir. Çalışmamızın ikinci bölümünde kemençe beşlemesindeki çalgılar, müzik terimleri, anahtar ve donanım kullanımları ile çok seslilik anlayışı analiz edilmiştir. Ayrıca kemençe beşlemesinde ney çalgısına yer verilmesi, Arel’in Batı müziğini emsal alarak yaptığı proje doğrultusunda, Batı müziği formları üzerinden tartışılmıştır. Arel-Ezgi-Uzdilek (AEU) sisteminde bir sekizli içinde 24 perde olması ve aralıkların eşit olmaması sebebiyle, eserin armonik analizinde karşımıza çıkan akor tipleri tespit edilmiş ve analizler bu yöntem doğrultusunda tonal armoni analizi kapsamında yapılmıştır. Bu meyanda Arel’in besteciliği ve kemençe beşlemesinin çok seslilik anlayışı incelenmiş ve kendi sistemine uyan ve uymayan yapılar tespit edilerek örneklerle açıklanmıştır. Bu eksende çalışmamız kemençe beşlemesindeki müzikal analizin ilk defa ele alınması açısından önem arz etmektedir.
Prototype and Design of Six Axis Robotic Manipulator
Authors: Pająk Mateusz , Racław Marcin , Piotrowski Robert
Published: March 2023

The paper presents a design of six axis manipulator. The design consists of specially designed solutions for housing, planetary gearboxes and electronics. The manipulator is controlled by a supervisory control system. The use of a series of measuring elements allows to track the current position of each axis  and use this to create a cascade control loop with velocity and acceleration feed-forward. The implemented control algorithm together with the microcontroller software allows to tune parameters of the controllers for both control loops: inner, related to the speed of the robot and outer, related to it’s position.
Odnos in pripravljenost zaposlenih v zdravstveni negi za oskrbo pacientov s HIV/AIDS-om
Authors: Plahuta Ana , Skrt Sanja , Jazbec Julija , Ličen Sabina
Published: March 2023

Uvod: Medicinske sestre predstavljajo rizični del prebivalstva, saj se neposredno srečujejo s telesnimi tekočinami pacientov, okuženih z virusom HIV ali obolelimi z AIDS-om. Namen raziskave je bil ugotoviti, kakšen je profesionalni in afektivni odnos zaposlenih v zdravstveni negi do pacientov, okuženih z virusom HIV ali obolelih z AIDS-om.Metode: V opisni neeksperimentalni kvantitativni raziskavi je bil uporabljen v slovenščino preveden vprašalnik Lestvica odnosa do obolelih z AIDS-om (Cronbach α = 0,732). Sodelovalo je 164 zaposlenih v zdravstveni negi iz slovenskih zdravstvenih ustanov. Zbiranje podatkov je potekalo s spletnim vprašalnikom od novembra 2020 do januarja 2021. Podatki so bili analizirani z deskriptivno statistiko, Mann-Whitneyjevim U-testom in Kruskal-Wallisovim testom. Upoštevana je bila stopnja statistične značilnosti (p < 0,05).Rezultati: Zaposleni v zdravstveni negi izražajo pozitiven profesionalni in afektivni odnos do pacientov okuženih z virusom HIV ali obolelih z AIDS-om. Pozitivnejši odnos izražajo moški, zaposleni z visokošolsko izobrazbo ali magisterijem in zaposleni z delovno dobo od pet do petnajst let. Pri omenjenih spremenljivkah razlike v odnosih niso bile statistično značilne (p > 0,05).Diskusija in zaključek: Iz rezultatov raziskav povzemamo, da medicinske sestre v Sloveniji ne izražajo izrazito pozitivnega ali negativnega odnosa do oseb, okuženih z  virusom HIV ali obolelih z AIDS-om, in so jim pripravljene nuditi holistično zdravstveno nego. V prihodnje bi si želeli raziskovanje osrediniti v merjenje splošnega znanja o HIV/AIDS-u med zaposlenimi in ugotoviti, koliko vpliva  znanje na odnos zaposlenih do obolelih.
Vloga izvajalcev zdravstvene nege pri pronaciji in supinaciji intubiranega pacienta s hudim akutnim respiratornim sindromom koronavirus-2
Authors: Halec Bine , Tisaj Eva
Published: March 2023

Uvod: Hud akutni respiratorni sindrom koronavirus-2 je s svojo intenzivnostjo močno vplival na razmere in delo v enotah intenzivne terapije. Pri vse več intubiranih pacientih z akutnim respiratornim distresnim sindromom se je pokazala potreba po pronaciji (obrat na trebuh). Namen raziskave je bil preučiti vlogo izvajalcev zdravstvene nege pri pronaciji in supinaciji (obrat na hrbet) pacienta ter ugotoviti najpogostejše zaplete, ki se ob tem lahko pojavijo.Metode: Uporabljen je bil pregled znanstvene in strokovne literature. Iskanje literature je potekalo v podatkovnih bazah PubMed, Sage in ScienceDirect ter Google Scholar. Vključene so bile raziskave od januarja 2020 do decembra 2021. Izvedena je bila vsebinska analiza raziskav.Rezultati: Skupno je bilo identificiranih 993 člankov. Dodanih je bilo še deset člankov, najdenih s prostoročnim iskanjem v Google Scholar. Izmed 1.003 identificiranih zadetkov je bilo v končno analizo vključenih osem člankov. Izpostavljena so bila tri glavna vsebinska področja: (1) vloga izvajalcev zdravstvene nege pred pronacijo intubiranega pacienta; (2) vloga izvajalcev zdravstvene nege med pronacijo intubiranega pacienta in (3) vloga izvajalcev zdravstvene nege pri supinaciji intubiranega pacienta.Diskusija in zaključek: Pronacija in supinacija intubiranega pacienta predstavljata velik izziv za celoten zdravstveni tim, ki je v času epidemije okrnjen tako številčno kot tudi glede na stopnjo usposobljenosti. Izvajalec zdravstvene nege mora ob teh intervencijah zagotoviti hemodinamsko stabilnost, pravilno pozicioniranje pacienta, preprečiti ekstubacijo in izpade različnih katetrov, zaščititi kožo in vseskozi izvajati potrebne aktivnosti zdravstvene nege.
Medosebni odnos med mentorjem in mentorirancem v klinični praksi zdravstvene nege
Authors: Nastran Ana , Praprotnik Deja , Renko Jakob , Ličen Sabina , Prosen Mirko
Published: March 2023

Uvod: Medosebni odnos med mentorjem in mentorirancem je ključnega pomena za uspešno izpeljan proces mentoriranja in izobraževanje novega kadra na  področju zdravstvene nege. Namen raziskave je bil preučiti in raziskati medosebne odnose med mentorji in  mentoriranci zdravstvene nege ter ugotoviti, kateri dejavniki krojijo njihove odnose.Metode: Uporabljena je bila kvalitativna metodologija  raziskovanja in deskriptivno-interpretativni dizajn. Podatki na namenskem vzorcu štirih mentorjev so bili pridobljeni z individualnimi delno strukturiranimi intervjuji in na vzorcu osmih mentorirancev s pomočjo dveh fokusnih skupin. Podatki so bili zbrani v decembru 2021 in analizirani z metodo analize vsebine.Rezultati: Ugotovljeno je bilo, da mentorji in mentoriranci njihov medosebni odnos dojemajo podobno. Identificirali smo skupno glavno temo »medosebni odnos mentor – mentoriranec« ter glavni podtemi »značilnosti  medosebnega odnosa mentor – mentoriranec« in »dejavniki medosebnega odnosa mentor – mentoriranec«. Tako mentorji kot mentoriranci imajo skladna pričakovanja glede njihove vloge. Kot glavne dejavnike medosebnega odnosa so oboji izpostavili osebnostne lastnosti mentorja in mentoriranca, postavljanje mej v medosebnem odnosu, čas, namenjen vzpostavljanju medosebnega odnosa, ter razlike v odnosu glede na področje kliničnega  usposabljanja.Diskusija in zaključek: Ugotovljeni so bili ključne značilnosti in dejavniki medosebnega odnosa med mentorjem in mentorirancem v procesu mentoriranja. Izpostavili smo ključne elemente, ki se jih morajo v procesu kliničnega usposabljanja zavedati vsi udeleženci, saj le tako lahko premostijo različne potencialne ovire in izboljšajo  izkušnjo mentoriranja v kliničnem okolju.
Doživljanje neizvedene zdravstvene nege pri medicinskih sestrah
Authors: Bahun Mateja , Jeriček Klanšček Helena
Published: March 2023

Uvod: Stiske zaradi zdravstvene nege, ki ostaja zaradi različnih razlogov neizvedena, so pri medicinskih sestrah pogosto spregledane in neprepoznane. Namen integrativnega pregleda literature je pregledati najnovejše raziskave, ki opisujejo, kako se neizvedena zdravstvena nega izraža na duševnem počutju medicinskih sester.Metode: Uporabili smo integrativni pregled literature, izveden v skladu s PRISMA smernicami v podatkovnih bazah PubMed, CINAHL, Wiley, COBISS.SI in Google Učenjak. Potek iskanja in analize literature je prikazan s pomočjo PRISMA diagrama. Zadetke smo razvrstili glede na raven dokazov. Uporabili smo tematsko analizo vsebine.Rezultati: Skupaj je bilo identificiranih 175 in v končno analizo vključenih 11 virov. Identificirano je bilo 96 kod združenih v 4 kategorije: (1) Telesno, duševno in čustveno počutje, (2) Moralno etične dileme, (3) Sprejemanje odločitev pri delu – prilagajanje, (4) Poklicne in osebne vrednote.Diskusija in zaključek: Neizvedena zdravstvena nega je za medicinske sestre pomemben izvor stisk in stresa, saj zaradi neizvajanja vseh potrebnih aktivnosti v zdravstveni negi prihajajo v neskladje s svojimi osebnimi in poklicnimi vrednotami. Naši rezultati dodajajo vsebinski kontekst in boljše razumevanje posledic neizvedene zdravstvene nege pri medicinskih sestrah. Vsakodnevno odločanje, katere aktivnosti bo potrebno izpustiti, vpliva ne samo na slabše počutje, nezadovoljstvo in izgorelost, ampak celo na zapuščanje delovnih mest in poklica.
Bioactive compounds and therapeutic properties of Aloe vera- a review
Authors: Mitra Abhirup , Singh Manali , Banga Akshita , Pandey Jyoti , Tripathi Sant Sharan , Singh Devraj
Published: March 2023

Aloe vera is a popular herbal medicine and worldwide appreciated for its therapeutic potential. From ancient time, it has been used as a home remedy for different health issues. Many researchers have shown its therapeutic potential and there are many citations of characterization of bioactive compounds from it. A wide range of bioactive compounds have been characterized from Aloe vera. It has a wide range of antimicrobial, antioxidant, and antidiabetic properties. Aloe vera can also provide support for carbohydrate and lipid metabolism by maintaining the level of cholesterol and sugar in blood and it can also help to maintain body weight. Due to the presence of higher number of bioactive compounds and prolific therapeutic properties, it is widely used in medicine, cosmetics and food sector. In this review, the characterisation of bioactive compounds from Aloe vera along with the extraction of its gel and encapsulation is reviewed with a discussion on its medicinal properties.
Priming-mediated triggering of antioxidative response to induce drought tolerance in Maize (Zea mays L.)
Authors: Thongbam Satyajyoti , Sinam Vikas , Mentada Bala Abhinav Sai , Kalangutkar Akshaya Mahadev , Siddique Anaytullah
Published: March 2023

Drought is a well-known issue in plants and it occurs when plants do not receive enough water to meet their requirements. Hence it alters the metabolic process of the plant, consequently reducing the yield. To overcome the loss of yield under prevailing situations, triggering of the antioxidative defense system is required which can mitigate the impact of drought on plants. The priming chemicals KNO3, Mg(NO3)2 and GA3 were evaluated with hydro-priming to know the mitigative response of priming against drought-induced stress in Maize plants. The morpho-physiological and biochemical parameters were used to evaluate the impact of priming-mediated triggers on the antioxidative response. The results of this work indicate that leaf area index (LAI), crop growth rate (CGR, mg g-1 day-1), total chlorophyll, and chl‘a’ (mg g-1) were recorded maximum in T5 (Mg(NO3)2, 10 mM) while chl ‘b’ in T4 (Mg (NO3)2, 7 mM). The maximum Membrane Stability Index (MSI %) and Membrane Injury Index (MII%) were recorded in T5 and T0 (Control). The osmoregulatory compound proline content (µg g-1) and antioxidative enzyme catalase (nm H2O2 mg-1 min-1) were detected in significantly highest quantity in T3 (KNO3, 15 mM) while the least amount of malondialdehyde (MDA nm g-1) was found under the same treatment. The correlation studies amongst all the parameters reflected that MII % and MDA content (MDA nm g-1) negatively correlated with the remaining parameters studied. This study has reflected that out of all the sources of priming treatments, KNO3 in 15 mM and Mg(NO3)2 in 10 mM has the potential to trigger the antioxidative defense mechanism to mitigate the response of drought in Maize.
Evaluation of morphophysiological, biochemical and antioxidant activity of green gram (Vigna radiata (L.) R.Wilczek) in responses to gamma irradiation
Authors: Vasudevan Soundarya , Dhanarajan Arulbalachandran , Kasim Yasmin , Sankar Vanmathi , Govindasamy Balasubramani , Gurunathan Selvakumar
Published: March 2023

Mutation breeding plays a vital role as a source of genetic diversity to improve plant growth and development. Green gram (Vigna radiata (L.) R. Wilczek) “Vamban 2” variety was selected for this investigation. The doses applied to the healthy seeds of green gram were 100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy, 600 Gy, 700 Gy and 800 Gy and non-treated seeds were kept as control throughout this study. A lethal dose (LD50) was observed at 500 Gy, whereas seedling length, fresh and dry weight decreased as the applied doses increased while compared to the control. Irradiated seedlings showed a decreased content of chlorophyll a compared to Chlorophyll b and increased carotenoid content compared to the control. Biochemical characteristics such as reducing sugar, starch, protein, amino acid and proline content were increased and noted maximum at 800 Gy. Antioxidants and lipid peroxidation (MDA) increased gradually along with increasing doses. FTIR analysis exhibited maximum functional groups at 600 Gy and ESR data showed ample hyperfine range of structure at 500 Gy, 600 Gy and 700 Gy. This investigation found considerable alterations in morphology, photosynthetic pigment, biochemical characteristics and antioxidant analyses, which suggest an idea to select an appropriate dose of gamma irradiation in green gram for successive breeding program.
Molecular phylogenetic analysis of Tulipa (Liliaceae) from Aksu-Zhabagly Nature Reserve
Authors: Sarsen Arailym , Saginova Malika , Akishev Zhiger , Aktayeva Saniya , Manabayeva Shuga , Khassenov Bekbolat
Published: March 2023

Barcodes are conserved sequences of genomic, plastid and mitochondrial DNA that can be utilized to uniquely identify an unidentified specimen to its species when conventional identification methods are inapplicable. Among prokaryotic and eukaryotic species, nuclear ribosomal internal transcribed spacer (ITS) sections are one of the most often utilized DNA markers in DNA barcoding and phylogenetic research. In addition to the ribosomal genes, the plastid genes are the most suitable for identifying plant species. The Aksu-Zhabagly Nature Reserve is the oldest nature reserve in Central Asia and is home to 1,312 vascular plant species, 44 of which are categorized as threatened or endangered in Kazakhstan's red data book. In this study, a collection of specimens of uncommon tulip species was compiled, along with their morphological identification and DNA barcoding. The ITS region and parts of the matK and ycf1b genes of tulip plastid DNA were sequenced. The evolutionary link between species of tulips was investigated. Phylogenetic study predicted two Tulipa subclades. Tulipa species have substantially preserved MatK genes. Tulips' ycf1b gene has evolved more slowly than other Liliaceae family members. Nuclear and plastid DNA sequences investigated Tulipa species evolutionary relationships. The findings about the ITS region of nuclear DNA were more definite. Overall, our work shows that genetic data will be important in determining species concepts in this genus, however, even with a molecular perspective pulling apart closely related taxa can be extremely challenging.
Effect of Leather Tanning Effluent on Chromium Contamination in Paddy Fields
Authors: Fadilah Kabul , Kaniawati Rosada Keukeu , Aziz Affandy Muhammad , Ali Munawar , Sunardi Sunardi
Published: March 2023

Leather tanning industry effluent contains high chromium (Cr) concentrations and poses environmental threats. In this study, we investigated the impact of leather tanning effluent contamination in water, soil, paddy, its grain and and its other morphometric parameters under the irrigated areas in the Ciwalen River, West Java. The result showed high Cr concentration in Ciwalen water stream, soil and paddy. The higher Cr concentrations of soil and paddy were found in the upstream area and closer plot to the main river stream. The bioaccumulation factor (BAF) values were <1, indicating that paddy has a low ability to accumulate Cr. However, paddy morphometric parameters, including total length, root length, and the number of leaves, decreased with higher Cr concentrations affected by leather tanning effluents.
Quality control of marketed herbal products of Asparagus racemosus Willd. through high performance thin layer chromatography (HPTLC) analysis
Authors: Champati Bibhuti Bhusan , Padhiari Bhuban Mohan , Ray Asit , Jena Sudipta , Sahoo Ambika , Sahoo Tirthabrata , Panda Pratap Chandra , Nayak Sanghamitra
Published: March 2023

Asparagus racemosus Willd. is a valuable medicinal plant which is used all over the world. There are several marketed products of A. racemosus. The high demand for this herb has increased the risk of adulteration in its commercial products. The adulterated herbal products might pose serious ill effects on health. Therefore, it is necessary to check the quality of marketed products in terms of the presence of their major bioactive compounds. The present study aimed to carry out the qualitative and quantitative analysis of Shatavarin IV in marketed products of A. racemosus through a validated high performance thin layer chromatography (HPTLC) method. Ten marketed products were analysed and all of them had shown the presence of Shatavarin IV which was quantified. The identification and quantification were done by taking a standard Shatavarin IV as reference. The Shatavarin IV was detected at Rf 0.4±0.05 and showed maximum absorption at 425 nm. The Shatavarin IV was quantified using a 6-point calibration curve having a standard deviation of 3.89 % with an R2 value of 0.9968. The amount of Shatavarin IV varied between 1.47±0.25 to 2.69±0.51 mg/g on a dry weight basis which is a normal range in the raw plant materials. Thus, the present findings would be a simple, reliable and cost-effective method for the quality determination of herbal products of A. racemosus. The developed HPTLC chromatograms would serve as a reference for the quality assessment of commercial products of A. racemosus in future.
DMPK studies in rat model for comparative evaluation of bioavailability of alpha-mangostin and its formulated solid lipid nanoparticle using a validated LC-MS/MS method
Authors: Vutti Nagendra Babu , GSN Koteswara Rao , Alavala Rajasekhar Reddy , Sultana Md. Jaha , Govada Kishore Babu , Nagendra CH , Madireddy Ravi Kumar
Published: March 2023

Garcinia mangostana L., contains the xanthone ?-mangostin, which is a bioactive secondary metabolite. The Caco-2 cell line transport of ?-mangostin was explored to see whether it could be used to study oral uptake. There has been little in-vivo research on the drug metabolism and pharmacokinetics of solid lipid nanoparticles of ?-mangostin. The ?-mangostin content estimation in plasma of rat was accomplished using a validated LC-MS/MS technique. The Papp (permeability coefficient apparent) across the Caco-2 cell monolayer is used to predict the absorption of orally administered ?-mangostin and ?-mangostin solid lipid nanoparticles (AM-SLNP). In the presence of the solid lipid and emulsifiers, AM-SLNP had 3.72 times higher Papp than ?-mangostin after 4 hours of study across the Caco-2 cell line. In-vivo rat model study show that formulated AM-SLNP has a 3.3-fold higher bioavailability than pure ?-mangostin. High tissue distribution of the AM-SLNP is observed compared to ?-mangostin, which may improve the efficacy of the product when compared to pure extract, as the available drug at the site of distribution is high. Because both cell monolayer and animal studies demonstrate the same pattern of drug intake mechanism for SLNP’s and as it is almost identical, nanotechnology can be utilized in avoiding hepatic metabolism and improving bioavailability.
Variation of the phenolic composition and a-glucosidase inhibition potential of seeds, soaked seeds, and sprouts of four wild forms and four varieties of common bean (Phaseolus vulgaris)
Authors: Almaraz-Abarca Norma , Ayala-Chaidez Ana Isabel , Wallander-Compeán Liliana , Ávila-Reyes Jose Antonio , Delgado-Alvarado Eli Amanda , Naranjo-Jiménez Néstor , Rosas-Medina Imelda , Colmenero-Robles Aurelio
Published: March 2023

The determination of the changes in the composition of bioactive phenolic compounds of germinating seeds which accumulate high levels of these compounds could contribute to the understanding of the germination mechanism and the development of markers for the selection of plant genotypes. In the current study, the changes in the phenolic composition and a-glucosidase inhibition activity, taking place during the germination of four wild forms and four varieties of common bean (Phaseolus vulgaris L.) from Durango Mexico, were determined. A total of 66 phenolic compounds (19 phenolic acids, 18 isoflavones, 18 flavonol glycosides, 3 flavonol aglycones, 3 flavones, 2 dihydroflavonoids, 2 chalcones and one non-identified type) were found by HPLC-DAD, which were differentially accumulated by the seeds, 24 h-soaked seeds, and 4 day-sprouts of each genotype. The accumulation of the flavonol aglycones, myricetin, quercetin and kaempferol was distinctive of the wild seeds. Soaking not only caused leaching and degradation but also triggered the synthesis of new phenolic compounds whereas germination diversified the composition of isoflavones and flavonol glycosides. The seeds of all genotypes analyzed were important inhibitors of a-glucosidase, improving their potential after soaking and germination. The results suggested that the structure rather than the concentration of the flavonoids and phenolic acids determined the inhibitory potential of a-glucosidase of samples. The principal component analysis and cluster analysis revealed HPLC-DAD phenolic profiles as genotype-specific chemomarkers at any of the states (seeds, soaked seeds, and sprouts). The results have wide implications on agronomy and food quality.
Mechanisms of Dominant Electrophysiological Features of Four Subtypes of Layer 1 Interneurons
Authors: Meng John Hongyu , Schuman Benjamin , Rudy Bernardo , Wang Xiao-Jing
Published: March 2023

Neocortical layer 1 (L1) consists of the distal dendrites of pyramidal cells and GABAergic interneurons (INs) and receives extensive long-range “top-down” projections, but L1 INs remain poorly understood. In this work, we systematically examined the distinct dominant electrophysiological features for four unique IN subtypes in L1 that were previously identified from mice of either gender: Canopy cells show an irregular firing pattern near rheobase; neurogliaform cells are late-spiking, and their firing rate accelerates during current injections; cells with strong expression of the α7 nicotinic receptor (α7 cells), display onset (rebound) bursting; vasoactive intestinal peptide (VIP) expressing cells exhibit high input resistance, strong adaptation, and irregular firing. Computational modeling revealed that these diverse neurophysiological features could be explained by an extended exponential-integrate-and-fire neuron model with varying contributions of a slowly inactivating K+channel, a T-type Ca2+channel, and a spike-triggered Ca2+-dependent K+channel. In particular, we show that irregular firing results from square-wave bursting through a fast-slow analysis. Furthermore, we demonstrate that irregular firing is frequently observed in VIP cells because of the interaction between strong adaptation and a slowly inactivating K+channel. At last, we reveal that the VIP and α7 cell models resonant with alpha/theta band input through a dynamic gain analysis.

SIGNIFICANCE STATEMENTIn the neocortex, ∼25% of neurons are interneurons. Interestingly, only somas of interneurons reside within layer 1 (L1) of the neocortex, but not of excitatory pyramidal cells. L1 interneurons are diverse and believed to be important in the cortical–cortex interactions, especially top-down signaling in the cortical hierarchy. However, the electrophysiological features of L1 interneurons are poorly understood. Here, we systematically studied the electrophysiological features within each L1 interneuron subtype. Furthermore, we build computational models for each subtype and study the mechanisms behind these features. These electrophysiological features within each subtype should be incorporated to elucidate how different L1 interneuron subtypes contribute to communication between cortexes.
Alternative Splicing of the Flip/Flop Cassette and TARP Auxiliary Subunits Engage in a Privileged Relationship That Fine-Tunes AMPA Receptor Gating
Authors: Perozzo Amanda M. , Brown Patricia M.G.E. , Bowie Derek
Published: March 2023

Alternative splicing of AMPA-type glutamate receptors (AMPARs) and allosteric modulation by auxiliary subunits, such as transmembrane AMPAR regulatory proteins (TARPs), are two important mechanisms that regulate the time course of glutamatergic neurotransmission. Prior work has shown that alternative splicing of the flip/flop cassette profoundly regulates TARP γ2 modulation, where flip receptor gating exhibits robust sensitivity to TARPs while flop isoforms are relatively insensitive to TARP modulation. Whether this splice variant-specific regulation extends to other auxiliary subunit families, such as cornichons (CNIHs), GSG1L, or CKAMPs, remains unknown. Here, we demonstrate that CNIH-3 modulation is unaffected by AMPAR alternative splicing due to inherent differences in how CNIH-3 and TARP γ2 modify channel gating. CNIH-3 slows receptor deactivation from the outset of current decay, consistent with structural evidence showing its point of contact at the level of the pore. In contrast, TARP γ2 acts via the KGK site of the ligand-binding domain (LBD) to slow the onset of desensitization. Although GSG1L and CKAMP44 primarily slow recovery from desensitization, their effects on channel gating are unaffected by alternative splicing, further underlining that structural events leading to the onset and recovery from desensitization are separable. Together, this work establishes that alternative splicing and TARP auxiliary subunits form a unique partnership that governs fast glutamatergic signaling at central synapses. Since proteomic studies suggest that all native AMPARs co-assemble with at least two TARPs, allosteric coupling between the flip/flop cassette and TARPs may represent a common design element in all AMPAR complexes of the mammalian brain.

SIGNIFICANCE STATEMENTAll fast excitatory neurotransmission in the mammalian brain is mediated by AMPA-type glutamate receptors (AMPARs). The time course of AMPAR gating can be regulated by two distinct mechanisms: alternative splicing of the flip/flop cassette and association with auxiliary subunits. Although these regulatory mechanisms have been well studied individually, it is not clear whether alternative splicing impacts auxiliary protein modulation of AMPARs. Here, we compare the four main families of AMPAR auxiliary subunits, transmembrane AMPAR regulatory proteins (TARPs; γ2), cornichons (CNIH-3), GSG1L and CKAMPs (CKAMP44), and find a privileged relationship between TARPs and the flip/flop cassette that is not shared by others. The flop cassette acts as a master switch to override TARP action, and this coupling represents a way to fine-tune AMPAR signaling.
Komplex endovascularis rekonstrukciók az aortaíven.
Authors: Nagy Martin Gellért , Hüttl Artúr , Borzsák Sarolta , Pólos Miklós , Szabolcs Zoltán , Csikós Gergely , Szeberin Zoltán , Sótonyi Péter , Csobay-Novák Csaba
Published: March 2023

Az aortaívet érintő aortabetegségek miatt végzett endovascularis rekonstrukciók
(thoracic endovascular aneurysm repair – TEVAR) során a sztentgraft proximalis
rögzítése az ívben vagy az aorta ascendensen van. Ilyen esetben hagyományosan
nyitott műtéttel előzetesen biztosítjuk a lefedésre kerülő supraaorticus ágak
keringését (ún. ’debranching’ műtétek). Nyitott műtétre nem alkalmas betegek
esetén azonban az ágak endovascularis módszerekkel történő megtartására
kényszerülünk. Tanulmányunkban ezen komplex endovascularis
aortaív-rekonstrukciók lehetőségeit mutatjuk be. A párhuzamos graftokat
jellemzően sürgősségi körülmények között alkalmazzuk. Az ascendensen történő
proximalis rögzítés esetén a jobb arteria (a.) carotisról indított ’debranching’
és a truncus brachiocephalicus párhuzamos grafttal történő biztosításával
kombinált hibrid műtétet végeztünk. Létfontosságú ér véletlen lefedésével járó
TEVAR esetén sürgősséggel végezhetünk konverziót például a bal a. carotis
communis keringésének gyors helyreállítására. A bal a. subclavia előzetes
revascularisatiója nélkül végzett sürgősségi TEVAR után ritkán jelentkező bal
felső végtagi ischaemia esetén utólagos konverziót végezhetünk a bal a.
subclavia lumenének helyreállítására ugyancsak párhuzamos grafttal. A
kisgörbületen elhelyezkedő, saccularis morfológiájú penetráló aortafekélyek
sikeres kirekesztését segítheti egyedi gyártású graft alkalmazása, melyen a
nagygörbületen lévő supraaorticus érszájadék köré kivágást, ún. ’scallop’-ot
helyezünk a graft proximalis végéhez, megnövelve így a proximalis nyak hosszát.
Elektív körülmények között ugyancsak egyedileg gyártott elágazó graftot is
alkalmazhatunk, melynek során akár mindhárom ág megtartható az ascendensről
induló proximalis rögzítés mellett, így arra alkalmas anatómia esetén
endovascularis ívcserére is lehetőségünk van. Orv Hetil. 2023; 164(11):
426–431.