bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of CFD Simulation to UAS Measurements for Wind Flows in Complex Terrain: Application to the WINSENT Test Site
Authors: El Bahlouli Asmae , Rautenberg Alexander , Schön Martin , zum Berge Kjell , Bange Jens , Knaus Hermann
Published: May 2019

This investigation presents a modelling strategy for wind-energy studies in complex terrains using computational fluid dynamics (CFD). A model, based on an unsteady Reynolds Averaged Navier-Stokes (URANS) approach with a modified version of the standard k-ε model, is applied. A validation study based on the Leipzig experiment shows the ability of the model to simulate atmospheric boundary layer characteristics such as the Coriolis force and shallow boundary layer. By combining the results of the model and a design of experiments (DoE) method, we could determine the degree to which the slope, the leaf area index, and the forest height of an escarpment have an effect on the horizontal velocity, the flow inclination angle, and the turbulent kinetic energy at critical positions. The DoE study shows that the primary contributor at a turbine-relevant height is the slope of the escarpment. In the second step, the method is extended to the WINSENT test site. The model is compared with measurements from an unmanned aircraft system (UAS). We show the potential of the methodology and the satisfactory results of our model in depicting some interesting flow features. The results indicate that the wakes with high turbulence levels downstream of the escarpment are likely to impact the rotor blade of future wind turbines.
Investigation of Soot Formation in a Novel Diesel Fuel Burner
Authors: Palazzo Natascia , Kögl Matthias , Bauer Philipp , Mannazhi Manu Naduvil , Zigan Lars , Huber Franz Johann Thomas , Will Stefan
Published: May 2019

In the present work, a novel burner capable of complete pre-vaporization and stationary combustion of diesel fuel in a laminar diffusion flame has been developed to investigate the effect of the chemical composition of diesel fuel on soot formation. For the characterization of soot formation during diesel combustion we performed a comprehensive morphological characterization of the soot and determined its concentration by coupling elastic light scattering (ELS) and laser-induced incandescence (LII) measurements. With ELS, radii of gyration of aggregates were measured within a point-wise measurement volume, LII was employed in an imaging approach for a 2D-analysis of the soot volume fraction. We carried out LII and ELS measurements at different positions in the flame for two different fuel types, revealing the effects of small modifications of the fuel composition on soot emission during diesel combustion.
Improved Rotor Braking Protection Circuit and Self-Adaptive Control for DFIG during Grid Fault
Authors: Chen Jiming , Wang Yuanhao , Zhu Mingxiao , Yu Qianyu , Li Jiacai
Published: May 2019

This paper introduces an improved rotor braking protection circuit configuration and the corresponding self-adaptive control strategy to enhance the low voltage ride-through (LVRT) capability of the doubly-fed induction generator (DFIG). The proposed protection circuit consists of a crowbar circuit and a series rotor braking resistor array, which guarantees the safe operation of wind generators under the LVRT. Moreover, to adapt the proposed protection and further enhance the performance of the improved configuration, a corresponding self-adaptive control strategy is presented, which regulates the rotor braking resistor and protection exiting time automatically through calculating the rotor current in the fault period. The LVRT capability and transient performance of the DFIG by using the proposed method is tested with simulation. Compared with the conventional crowbar protection or the fixed rotor braking protection, the proposed protection and the control strategy present several advantages, such as retaining the control of the rotor side converter, avoiding repeated operation of the protection and accelerating the damping of stator flux linkage during a grid fault.
Methane Emission during Gas and Rock Outburst on the Basis of the Unipore Model
Authors: Norbert Skoczylas , Pajdak Anna , Kozieł Katarzyna , Braga Leticia Teixeira Palla
Published: May 2019

The goal of this paper is to analyze the phenomenon of gas emission during a methane and coal outburst based on the unipore Crank diffusion model for spherical grains and plane sheets. Two occurrences in the Upper Silesian Coal Basin were analyzed: an outburst in a Zofiówka coal mine in 2005 and an outburst in a Budryk coal mine in 2012. Those two outbursts differed considerably. The first one was connected with an unidentified tectonic disturbance in the form of a triple, interlocking fault, and the other one is an example of an outburst in an area free from tectonic disturbances. The model analysis required laboratory tests in order to determine the sorption properties of coals from post-outburst masses. Sorption isotherms and the values of the effective diffusion coefficient were specified. The post-outburst masses were subjected to sieve analysis and the grain composition curves were plotted. The researchers also used the measurement data provided by proper mine services, such as the methane content, the volume of post-outburst masses, and the time courses of CH4 concentration changes in excavations. They were recorded by methane measurement systems in the mines.
A Zero-Dimensional Mixing Controlled Combustion Model for Real Time Performance Simulation of Marine Two-Stroke Diesel Engines
Authors: Feng Yongming , Wang Haiyan , Gao Ruifeng , Zhu Yuanqing
Published: May 2019

The paper presents a performance prediction model of marine low-speed two-stroke diesel engines based on an advanced MCC (mixture controlled combustion) model coupled with a fuel injection model. Considering the time of real calculation, the so-called “concentrated exhausting gas” scavenging model and the working process model are used in the present work, and improved by introducing the ratio of pure combustion product over the total gas mass in the cylinder as an expression of the working medium components. The reaction rate model in the zero-dimensional MCC model is improved by introducing the fraction of combustion product in the fuel spray, and the relationship between the combustion model and scavenging quality is established. Meanwhile, the combustion model was simplified in the diffusion combustion phases and integrated with the fuel injection model in order to respond to the change of injection profile and injection timing. A large-scale low-speed marine diesel engine was used for a simulation. The results of the whole model are consistent with experimental data and the speed of calculation is fast enough for real time simulation of low speed and medium speed diesel engines. The prediction model can be used in the design and calibration of the electronic control system and performance optimization of the marine two-stroke diesel engine.
The Economics of Rapid Multiplication of Hybrid Poplar Biomass Varieties
Authors: Stanton Brian J. , Haiby Kathy , Gantz Carlos , Espinoza Jesus , Shuren Richard A.
Published: May 2019

Background: Poplar (Populus spp.) hybridization is key to advancing biomass yields and conversion efficiency. Once superior varieties are selected, there is a lag in commercial use while they are multiplied to scale. Objective: The purpose of this study was to assess the influence of gains in biomass yield and quality on investment in rapid propagation techniques that speed the time to commercial deployment. Material and Methods: A factorial experiment of propagation method and hybrid variety was conducted to quantify the scale-up rate of in vitro and greenhouse clonal multiplication. These data were used in modeling the internal rate of return (IRR) on investment into rapid propagation as a function of genetic gains in biomass yield and quality and compared to a base case that assumed the standard method of supplying operational varieties in commercial quantities from nurseries as hardwood cuttings, capable of yields of 16.5 Mg ha−1 year−1. Results: Analysis of variance in macro-cutting yield showed that propagation method and varietal effects as well as their interaction were highly significant, with hedge propagation exceeding serial propagation in macro-cutting productivity by a factor of nearly 1.8. The Populus deltoides × P. maximowiczii and the Populus trichocarpa × P. maximowiczii varieties greatly exceeded the multiplication rate of the P. × generosa varieties due to their exceptional response to repeated hedging required to initiate multiple tracks of serial propagation. Analyses of investment into rapid propagation to introduce new material into plantation establishment followed by a 20-year rotation of six coppice harvests showed that gains in biomass yield and quality are warranted for a commitment to rapid propagation systems. The base case analysis was generally favored at yields up to 18 Mg−1 year−1 dependent on pricing. The rapid multiplication analysis proved superior to the base case analysis at the two highest yield levels (27.0 and 31.5 Mg ha−1 year−1,) at all price levels and at yields of 22.5 Mg−1 year−1, dependent on price and farm location. Conclusion: Rapid multiplication is a reliable method to move improved plant material directly into operations when valued appropriately in the marketplace.
Immediate Changes in Organic Matter and Plant Available Nutrients of Haplic Luvisol Soils Following Different Experimental Burning Intensities in Damak Forest, Hungary
Authors: Bridges Jack M. , Petropoulos George P. , Clerici Nicola
Published: May 2019

One of the major pedological changes produced by wildfires is the drastic modification of forest soil systems properties. To our knowledge, large research gaps are currently present concerning the effect of such fires on forest Haplic Luvisols soils in Central Europe. In this study, the effects of experimental fires on soil organic matter and chemical properties at different burning intensities in a Central European forest were examined. The study was conducted at Damak Forest, in Hungary, ecosystem dominated by deciduous broadleaf trees, including the rare Hungarian oak Quercus frainetto Ten. The experimental fires were carried out in nine different plots on Haplic Luvisol soils transferred from Damak Forest to the burning site. Three types of fuel load were collected from the forest: litter layer, understorey and overstorey. Groups of three plots were burned at low (litter layer), medium intensity (litter and understorey) and high intensity (litter, understorey and overstorey). Pre-fire and post-fire soil samples were taken from each plot, analysed in the laboratory and statistically compared. Key plant nutrients of organic matter, carbon, potassium, calcium, magnesium and phosphorus were analysed from each sample. No significant differences in soil organic matter and carbon between pre- and post-fire samples were observed, but high intensity fires did increase soil pH significantly. Calcium, magnesium and phosphorus availability increased significantly at all fire intensity levels. Soil potassium levels significantly decreased (ca. 50%) for all intensity treatments, in contrast to most literature. Potassium is a key nutrient for ion transport in plants, and any loss of this nutrient from the soil could have significant effects on local agricultural production. Overall, our findings provide evidence that support the maintaining of the current Hungarian fire prevention policy.
Viability of 4 Probiotic Bacteria Microencapsulated with Arrowroot Starch in the Simulated Gastrointestinal Tract (GIT) and Yoghurt
Authors: Samedi Lesly , Charles Albert Linton
Published: May 2019

Probiotic bacteria are usually encapsulated to increase their survival through passage of the simulated gastrointestinal tract (GIT). Four Lactobacilli were freeze-dried and encapsulated with maltodextrin (maltodextrin 1.25 g, whey 0.25 g, bacteria 0.5 g, and water 2 mL) and arrowroot starch (arrowroot 1.25 g, whey 0.25 g, bacteria 0.5 g, and water 2 mL). The effects of different coatings were evaluated for their viability in the GIT and yogurt. The findings indicated no significant differences at p > 0.05 in the survival of the encapsulated cells with increased concentrations of arrowroot and maltodextrin. The viability of the encapsulated bacteria was increased in the simulated GIT with high counts of 109 cfu/mL after 30 min stiffening in 1 µm size beads. However, the bead fermented yogurt exhibited insignificant difference on the survivability of the organisms in a simulated GIT after 15 days. Lactobacillus plantarum, Weissela paramesenteroides, Enterococcus faecalis, and Lactobacillus paraplantarum showed a significant increase of viable cells at p > 0.05 after freeze-drying in comparison with free cells at high bile salt concentrations and low acidity. This study confirmed that arrowroot starch and maltodextrin combinations in encapsulation might be an effective method that could allow viable probiotic bacteria to reach the large intestine.
Flux States of Active Galactic Nuclei
Authors: Dorner Daniela , Arbet-Engels Axel , Baack Dominik , Balbo Matteo , Biland Adrian , Blank Michael , Bretz Thomas , Bruegge Kai , Bulinski Michael , Buss Jens , Doerr Manuel , Elsaesser Dominik , Hildebrand Dorothee , Linhoff Lena , Mannheim Karl , Mueller Sebastian , Neise Dominik , Neronov Andrii , Noethe Maximilian , Paravac Aleksander , Rhode Wolfgang , Schleicher Bernd , Schulz Florian , Sedlaczek Kevin , Shukla Amit , Sliusar Vitalii , von Willert Elan , Walter Roland ,   FACT Collaboration
Published: May 2019

Blazars are known to show variability on time scales from minutes to years covering a wide range of flux states. Studying the flux distribution of a source allows for various insights. The shape of the flux distribution can provide information on the nature of the underlying variability processes. The level of a possible quiescent state can be derived from the main part of the distribution that can be described by a Gaussian distribution. Dividing the flux states into quiescent and active, the duty cycle of a source can be calculated. Finally, this allows alerting the multi-wavelength and multi-messenger community in case a source is in an active state. To get consistent and conclusive results from flux distributions, unbiased long-term observations are crucial. Only like this is a complete picture of the variability and flux states, e.g., an all-time quiescent state, possible. In seven years of monitoring of bright TeV blazars, the first G-APD Cherenkov telescope (FACT) has collected a total of more than 11,700 hours of physics data with 1500 hours to 3000 hours per source for Mrk 421, Mrk 501, 1ES 1959+650, and 1ES 2344+51.
Optimizing an Inner-Continental Shelf Geologic Framework Investigation through Data Repurposing and Machine Learning
Authors: Pendleton Elizabeth A. , Sweeney Edward M. , Brothers Laura L.
Published: May 2019

The U.S. Geological Survey (USGS) and the National Oceanic Atmospheric Administration (NOAA) have collected approximately 5400 km2 of geophysical and hydrographic data on the Atlantic continental shelf between Delaware and Virginia over the past decade and a half. Although originally acquired for different objectives, the comprehensive coverage and variety of data (bathymetry, backscatter, imagery and physical samples) presents an opportunity to merge collections and create high-resolution, broad-scale geologic maps of the seafloor. This compilation of data repurposes hydrographic data, expands the area of geologic investigation, highlights the versatility of mapping data, and creates new geologic products that would not have been independently possible. The data are classified using a variety of machine learning algorithms, including unsupervised and supervised methods. Four unique classes were targeted for classification, and source data include bathymetry, backscatter, slope, curvature, and shaded-relief. A random forest classifier used on all five source data layers was found to be the most accurate method for these data. Geomorphologic and sediment texture maps are derived from the classified acoustic data using over 200 ground truth samples. The geologic data products can be used to identify sediment sources, inform resource management, link seafloor environments to sediment texture, improve our understanding of the seafloor structure and sediment pathways, and demonstrate how ocean mapping resources can be useful beyond their original intent to maximize the footprint and scientific impact of a study.
New Feature Classes for Acoustic Habitat Mapping—A Multibeam Echosounder Point Cloud Analysis for Mapping Submerged Aquatic Vegetation (SAV)
Authors: Held Philipp , Schneider von Deimling Jens
Published: May 2019

A new method for multibeam echosounder (MBES) data analysis is presented with the aim of improving habitat mapping, especially when considering submerged aquatic vegetation (SAV). MBES data were acquired with 400 kHz in 1–8 m water depth with a spatial resolution in the decimeter scale. The survey area was known to be populated with the seagrass Zostera marina and the bathymetric soundings were highly influenced by this habitat. The depth values often coincide with the canopy of the seagrass. Instead of classifying the data with a digital terrain model and the given derivatives, we derive predictive features from the native point cloud of the MBES soundings in a similar way to terrestrial LiDAR data analysis. We calculated the eigenvalues to derive nine characteristic features, which include linearity, planarity, and sphericity. The features were calculated for each sounding within a cylindrical neighborhood of 0.5 m radius and holding 88 neighboring soundings, on average, during our survey. The occurrence of seagrass was ground-truthed by divers and aerial photography. A data model was constructed and we applied a random forest machine learning supervised classification to predict between the two cases of “seafloor” and “vegetation”. Prediction by linearity, planarity, and sphericity resulted in 88.5% prediction accuracy. After constructing the higher-order eigenvalue derivatives and having the nine features available, the model resulted in 96% prediction accuracy. This study outlines for the first time that valuable feature classes can be derived from MBES point clouds—an approach that could substantially improve bathymetric measurements and habitat mapping.
Area-Based Socio-Economic Inequalities in Mortality from Lung Cancer and Respiratory Diseases
Authors: Polak Maciej , Genowska Agnieszka , Szafraniec Krystyna , Fryc Justyna , Jamiołkowski Jacek , Pająk Andrzej
Published: May 2019

Background: After political transformation in 1989/1990, Poland experienced a general improvement in living conditions and quality of life, but the benefits did not extend evenly across all segments of the society. We hypothesized that the regional differences in mortality due to diseases of the respiratory system are related to socioeconomic status (SES) and its changes over time. Materials and methods: An ecological study was carried out in 66 sub-regions of Poland using the data from the period of 2010 to 2014. Age-standardized mortality rates (SMRs) were calculated separately for men and women in three age categories: ≥15, 25–64 years, and ≥65 years. An area-based SES index was derived from the characteristics of the sub-regions using the z-score method. Multiple weighted linear regression models were constructed to estimate a real socioeconomic gradient for mortality resulting from lung cancer and respiratory diseases. Results: In the regions studied, the SMRs for respiratory disease varied from 70/100,000 to 215/100,000 in men and from 18/100,000 to 53/100,000 in women. The SMRs for lung cancer varied from 36/100,000 to 110/100,000 among men and from 26/100,000 to 77/100,000 among women. After adjusting for the prevalence of smoking and environmental pollution, the SES index was found to be inversely associated with the SMR for lung cancer in each category of age among men, and in the age group of 25–64 years among women. An increase of the SES index between 2010 and 2014 was associated with a decrease of SMR for respiratory disease both in men and women, but this change was not significantly associated with the SMR for lung cancer. Conclusion: SES appears to be an important correlate of mortality from respiratory diseases and lung cancer at the population level, particularly in men. A lower SES was associated with greater mortality from lung cancer and respiratory diseases. An increase in SES over time was related to a decrease in mortality from respiratory disease, but not from lung cancer.
Oral Health-Related Quality of Life in Native and Immigrant Populations in the PELFI Study in Spain
Authors: Agudelo-Suárez Andrés A. , Vivares-Builes Annie M. , Muñoz-Pino Natalia , Martínez-Martínez José Miguel , Reid Alison , Ronda-Pérez Elena
Published: May 2019

Quality of life and its relationship to oral health is an important consideration in the determinants of health of vulnerable groups. The aim of this study is to assess oral health-related quality of life (OHRQoL) and its related factors in native and immigrant population families from the Platform of Longitudinal Studies on Immigrant Families (PELFI) study in Spain. A cross-sectional study was conducted in a sample of 401 adults aged 18 years and older from Spain, Ecuador, Colombia, and Morocco. The OHIP-14 instrument was applied, and three summary variables were used (prevalence, extent, and severity). Sociodemographic and self-perceived health variables were included. Bivariate analyzes were carried out to summarize the variables of the OHIP-14 according to sociodemographic and health variables, and bivariate analyzes of the OHIP-14 dimensions was conducted by country of origin. Multivariate linear models were used to investigate predictors for the dimensions of the OHIP-14. Multivariate logistic models were used to estimate the association of OHRQoL with immigration status using crude and adjusted odds ratios with 95% confidence intervals (OR-95% CI). It was found that 14.8% of men and 23.8% of women reported negative impacts in terms of OHRQoL (statistically significant differences: p < 0.05). There were statistically significant differences according to the country of origin in the prevalence and severity outcomes of the OHIP-14 in women (p < 0.05), and severe outcomes were observed in Moroccan women. In women, statistically significant differences (p < 0.05) in OHRQoL were observed according to age and marital status. There were some differences between OHIP-14 summary outcomes according to the health variables. Some sociodemographic and health variables were predictors for the OHIP-14 and their dimensions with differences by sex. Multivariate analysis showed a statistically significant association between OHRQoL and immigration status for Moroccan women. Differences in OHRQoL were found according to sociodemographic and health variables. Further research could clarify the predictors of OHRQoL through epidemiological surveillance and longitudinal studies.
The Perceived Impact of Suicide Bereavement on Specific Interpersonal Relationships: A Qualitative Study of Survey Data
Authors: Azorina Valeriya , Morant Nicola , Nesse Hedvig , Stevenson Fiona , Osborn David , King Michael , Pitman Alexandra
Published: May 2019

People bereaved by suicide have an increased risk of suicide and suicide attempt, yet report receiving less support than people bereaved by other sudden deaths. Reductions in support may contribute to suicide risk, yet their nature is unclear. We explored the impact of suicide bereavement on the interpersonal relationships of young adults in the UK using an online survey to collect qualitative data. We conducted thematic analysis of free-text responses from 499 adults to questions capturing the impact of bereavement on relationships with partners, close friends, close family, extended family, and other contacts. We identified four main themes describing the changes in relationships following the suicide: (1) Social discomfort over the death (stigma and taboo; painfulness for self or others to discuss; socially prescribed grief reactions); (2) social withdrawal (loss of social confidence; withdrawal as a coping mechanism); (3) shared bereavement experience creating closeness and avoidance; (4) attachments influenced by fear of further losses (overprotectiveness towards others; avoiding attachments as protective). These findings contribute to understanding deficits in support and pathways to suicidality after suicide bereavement. Such disrupted attachments add to the burden of grief and could be addressed by public education on how to support those bereaved by suicide.
Genetic Variants Associated with FDNY WTC-Related Sarcoidosis
Authors: Cleven Krystal L. , Ye Kenny , Zeig-Owens Rachel , Hena Kerry M. , Montagna Cristina , Shan Jidong , Hosgood H. Dean , Jaber Nadia , Weiden Michael D. , Colbeth Hilary L. , Goldfarb David G. , Spivack Simon D. , Prezant David J.
Published: May 2019

Sarcoidosis is a systemic granulomatous disease of unknown etiology. It may develop in response to an exposure or inflammatory trigger in the background of a genetically primed abnormal immune response. Thus, genetic studies are potentially important to our understanding of the pathogenesis of sarcoidosis. We developed a case-control study which explored the genetic variations between firefighters in the Fire Department of the City of New York (FDNY) with World Trade Center (WTC)-related sarcoidosis and those with WTC exposure, but without sarcoidosis. The loci of fifty-one candidate genes related to granuloma formation, inflammation, immune response, and/or sarcoidosis were sequenced at high density in enhancer/promoter, exonic, and 5’ untranslated regions. Seventeen allele variants of human leukocyte antigen (HLA) and non-HLA genes were found to be associated with sarcoidosis, and all were within chromosomes 1 and 6. Our results also suggest an association between extrathoracic involvement and allele variants of HLA and non-HLA genes found not only on chromosomes 1 and 6, but also on chromosomes 16 and 17. We found similarities between genetic variants with WTC-related sarcoidosis and those reported previously in sporadic sarcoidosis cases within the general population. In addition, we identified several allele variants never previously reported in association with sarcoidosis. If confirmed in larger studies with known environmental exposures, these novel findings may provide insight into the gene-environment interactions key to the development of sarcoidosis.
Self-Rated Health and Social Exclusion: Does Gardening Moderate This Relation? Evidence from the German Ageing Survey
Authors: Hajek André , König Hans-Helmut
Published: May 2019

The aim of the present study was to examine whether the association between self-rated health and social exclusion can be moderated by the frequency of gardening in the total sample and stratified by sex. Cross-sectional data employed in this study came from the fifth wave of the German Ageing Survey (n = 5048), a nationally representative sample comprising non-institutionalized individuals aged 40 and above. A single-item measure was used to quantify self-rated health (ranging from 1 = very good to 5 = very bad). An established scale developed by Bude and Lantermann was used to assess social exclusion. Moreover, individuals reported the frequency of work in the garden (daily; several times a week; once a week; 1-3 times a month; less often; never). Poorer self-rated health was associated with feelings of social exclusion. The frequency of gardening significantly moderated the association between these factors in women. This cross-sectional study emphasizes the moderating role of gardening in the relation between self-rated health and social exclusion in women. Longitudinal studies are required to validate the present findings.
How Older Adults Cope with Cognitive Complexity and Environmental Constraints during Dual-Task Walking: The Role of Executive Function Involvement
Authors: Forte Roberta , Pesce Caterina , Di Baldassarre Angela , Shea John , Voelcker-Rehage Claudia , Capranica Laura , Condello Giancarlo
Published: May 2019

This cross-sectional study investigated the interactive dual-task (DT) effects of executive function demands and environmental constraints on older adults’ walking and the moderating role of habitual physical activity (PA). Locomotor performance under different environmental constraints (flat versus obstructed walking) and cognitive performance with different executive function involvement (backward counting versus random number generation) were assessed under single-task (ST) and DT conditions in 135 participants (mean age 68.1 ± 8.4). The weekly number of daily steps was measured. Reciprocal DT effects of walking on cognitive performance and of the cognitive task on gait performance were computed and submitted to analyses of covariance with age, PA level, and cognitive functioning as covariates, followed by linear regressions with PA level as predictor. Cognitive task demands and environmental constraints individually and jointly affected gait variability (p = 0.033, ηp2 = 0.08) and executive function performance (p = 0.009, ηp2 = 0.09). Physical activity level predicted a low but significant percentage of variance of DT effects on gait only in flat walking (R2 = 0.04, p = 0.027). Results suggest that older individuals may adopt variable task prioritization in dual tasking depending on the type of executive function involvement and the environmental constraints on walking. Their DT ability was slightly affected by habitual PA.
Different Predictor Variables for Women and Men in Ultra-Marathon Running—The Wellington Urban Ultramarathon 2018
Authors: O’Loughlin Emma , Nikolaidis Pantelis T. , Rosemann Thomas , Knechtle Beat
Published: May 2019

Ultra-marathon races are increasing in popularity. Women are now 20% of all finishers, and this number is growing. Predictors of performance have been examined rarely for women in ultra-marathon running. This study aimed to examine the predictors of performance for women and men in the 62 km Wellington Urban Ultramarathon 2018 (WUU2K) and create an equation to predict ultra-marathon race time. For women, volume of running during training per week (km) and personal best time (PBT) in 5 km, 10 km, and half-marathon (min) were all associated with race time. For men, age, body mass index (BMI), years running, running speed during training (min/km), marathon PBT, and 5 km PBT (min) were all associated with race time. For men, ultra-marathon race time might be predicted by the following equation: (r² = 0.44, adjusted r² = 0.35, SE = 78.15, degrees of freedom (df) = 18) ultra-marathon race time (min) = −30.85 ± 0.2352 × marathon PBT + 25.37 × 5 km PBT + 17.20 × running speed of training (min/km). For women, ultra-marathon race time might be predicted by the following equation: (r² = 0.83, adjusted r2 = 0.75, SE = 42.53, df = 6) ultra-marathon race time (min) = −148.83 + 3.824 × (half-marathon PBT) + 9.76 × (10 km PBT) − 6.899 × (5 km PBT). This study should help women in their preparation for performance in ultra-marathon and adds to the bulk of knowledge for ultra-marathon preparation available to men.
Analysis of the Financing of Russian Health Care over the Past 100 Years
Authors: Reshetnikov Vladimir , Arsentyev Evgeny , Bolevich Sergey , Timofeyev Yuriy , Jakovljević Mihajlo
Published: May 2019

The evolution of epidemiological burden in Imperial Russia and, consecutively, the Union of Soviet Socialist Republics (USSR), took place mostly over the duration of the past century [...]
Vegetation Response to Groundwater Variation in Arid Environments: Visualization of Research Evolution, Synthesis of Response Types, and Estimation of Groundwater Threshold
Authors: Huang Feng , Zhang Danrong , Chen Xi
Published: May 2019

Groundwater depth is an important environmental factor affecting vegetation growth and landscape dynamics in arid environments. This study applied a science mapping approach to visualize the development of groundwater-vegetation-related research, synthesized the vegetation response to changes in groundwater depth, and analyzed the change rate of the response curve to identify the groundwater threshold that is essential to conserve the groundwater-dependent terrestrial ecosystems. These ecosystems emerged as a research hotspot due to climate change, groundwater overexploitation, and the recognition of these ecosystems’ importance for sustainable development. There are two main types of response functions of vegetation to changes in groundwater depth—monotone and bell-shaped functions—among which the monotone function includes linear, curvilinear, and stepwise response. The shape of a response curve is mainly determined by the combined effects of oxygen stress, salinization, and water stress; oxygen stress and salinization dominate in shallow groundwater depth, while water stress dominates in deep groundwater depth. On a non-linear vegetation metric—groundwater depth response curve, the change rate analysis method is effective to identify the breakpoint that can be taken as a candidate threshold of groundwater depth. The results will add insight into the intellectual structure of the groundwater-vegetation interactions and provide practical reference for groundwater resource management, ecological conservation, and sustainable development in arid environments.
Multi-Mode Two-Step Floating Catchment Area (2SFCA) Method to Measure the Potential Spatial Accessibility of Healthcare Services
Authors: Ni Jianhua , Liang Ming , Lin Yan , Wu Yanlan , Wang Chen
Published: May 2019

While great progress in the development of a methodological approach to measure the accessibility of healthcare services has been made, the exclusion of the complex multi-mode travel behavior of urban residents and a rough calculation of travel costs from the origin to the destination limit its potential for making a detailed assessment, especially in urban areas. In this paper, we aim to describe and implement an enhanced method that enables the integration of multiple transportation modes into a two-step floating catchment area (2SFCA) method to estimate accessibility. We used a travel-mode choice survey, based on distance sections, to determine the complex multi-mode travel behavior of urban residents. Taking Nanjing as a study area, we proposed complete door-to-door approaches to determine every aspect of basic transportation modes. Additionally, we processed open data to implement an accurate computing of the origin-destination (OD) time cost. We applied the enhanced method to estimate the accessibility of residents to hospitals and compared it with three single-mode 2SFCA methods. The results showed that the proposed method effectively identified more accessibility details and provided more realistic accessibility values.
Effects of Abscisic Acid and Salicylic Acid on Gene Expression in the Antiviral RNA Silencing Pathway in Arabidopsis
Authors: Alazem Mazen , Kim Kook-Hyung , Lin Na-Sheng
Published: May 2019

The RNA silencing pathways modulate responses to certain stresses, and can be partially tuned by several hormones such as salicylic acid (SA) and abscisic acid (ABA). Although SA and ABA are often antagonistic and often modulate different stress responses, they have similar effects on virus resistance, which are partially achieved through the antiviral RNA silencing pathway. Whether they play similar roles in regulating the RNA silencing pathway is unclear. By employing coexpression and promoter analyses, we found that some ABA- and SA-related transcription factors (TFs) are coexpressed with several AGO, DCL, and RDR genes, and have multiple binding sites for the identified TFs in the queried promoters. ABA and SA are antagonistic with respect to the expression of AGO1 and RDRs because ABA was able to induce these genes only in the SA mutant. Nevertheless, both hormones showed similarities in the regulation of other genes, for example, the induction of AGO2 by ABA was SA-dependent, indicating that ABA acts upstream of SA in this regulation. We inferred that the similar effects of ABA and SA on some genes resulted in the redundancy of their roles in resistance to bamboo mosaic virus, but that the two hormones are antagonistic with respect to other genes unrelated to their biosynthesis pathways.
Genome Editing as a Treatment for the Most Prevalent Causative Genes of Autosomal Dominant Retinitis Pigmentosa
Authors: Diakatou Michalitsa , Manes Gaël , Bocquet Beatrice , Meunier Isabelle , Kalatzis Vasiliki
Published: May 2019

Inherited retinal dystrophies (IRDs) are a clinically and genetically heterogeneous group of diseases with more than 250 causative genes. The most common form is retinitis pigmentosa. IRDs lead to vision impairment for which there is no universal cure. Encouragingly, a first gene supplementation therapy has been approved for an autosomal recessive IRD. However, for autosomal dominant IRDs, gene supplementation therapy is not always pertinent because haploinsufficiency is not the only cause. Disease-causing mechanisms are often gain-of-function or dominant-negative, which usually require alternative therapeutic approaches. In such cases, genome-editing technology has raised hopes for treatment. Genome editing could be used to (i) invalidate both alleles, followed by supplementation of the wild type gene, (ii) specifically invalidate the mutant allele, with or without gene supplementation, or (iii) to correct the mutant allele. We review here the most prevalent genes causing autosomal dominant retinitis pigmentosa and the most appropriate genome-editing strategy that could be used to target their different causative mutations.
Prodrugs for Skin Delivery of Menahydroquinone-4, an Active Form of Vitamin K2(20), Could Overcome the Photoinstability and Phototoxicity of Vitamin K2(20)
Authors: Goto Shotaro , Setoguchi Shuichi , Yamakawa Hirofumi , Watase Daisuke , Terada Kazuki , Matsunaga Kazuhisa , Karube Yoshiharu , Takata Jiro
Published: May 2019

The effective delivery of menahydroquinone-4 (MKH), an active form of menaquinone-4 (MK-4, vitamin K2(20)), to the skin is beneficial in the treatment of various skin pathologies. However, its delivery through the application of MK-4 to the skin is hampered due to the photoinstability and phototoxicity of MK-4. This study aimed to evaluate the potential of ester prodrugs of MKH for its delivery into the skin to avoid the abovementioned issues. The ester prodrugs, MKH 1,4-bis-N,N-dimethylglycinate hydrochloride (MKH-DMG) and MKH 1,4-bis-hemisuccinate (MKH-SUC), were prepared using our previously reported methods. Photostability was determined under artificial sunlight and multi-wavelength light irradiation, phototoxicity was determined by intracellular ROS formation and cell viability of UVA-irradiated human epidermal keratinocyte cells (HaCaT), and delivery of MKH into HaCaT cells was assessed by measuring menaquinone-4 epoxide (MKO) levels. MKH prodrugs showed higher photostability than MK-4. Although MK-4 induced cellular ROS and reduced cell viability after UVA irradiation, MKH prodrugs did not affect either ROS generation or cell viability. MKH prodrugs enhanced intracellular MKO, indicating effective delivery of MKH and subsequent carboxylation activity. In conclusion, these MKH prodrugs show potential for the delivery of MKH into the skin without photoinstability and phototoxicity.
Impact of Pharmacological Inhibition of Hydrogen Sulphide Production in the SOD1G93A-ALS Mouse Model
Authors: Spalloni Alida , Greco Viviana , Ciriminna Giulia , Corasolla Carregari Victor , Marini Federica , Pieroni Luisa , Mercuri Nicola B. , Urbani Andrea , Longone Patrizia
Published: May 2019

A number of factors can trigger amyotrophic lateral sclerosis (ALS), although its precise pathogenesis is still uncertain. In a previous study done by us, poisonous liquoral levels of hydrogen sulphide (H2S) in sporadic ALS patients were reported. In the same study very high concentrations of H2S in the cerebral tissues of the familial ALS (fALS) model of the SOD1G93A mouse, were measured. The objective of this study was to test whether decreasing the levels of H2S in the fALS mouse could be beneficial. Amino-oxyacetic acid (AOA)—a systemic dual inhibitor of cystathionine-β-synthase and cystathionine-γ lyase (two key enzymes in the production of H2S)—was administered to fALS mice. AOA treatment decreased the content of H2S in the cerebral tissues, and the lifespan of female mice increased by approximately ten days, while disease progression in male mice was not affected. The histological evaluation of the spinal cord of the females revealed a significant increase in GFAP positivity and a significant decrease in IBA1 positivity. In conclusion, the results of the study indicate that, in the animal model, the inhibition of H2S production is more effective in females. The findings reinforce the need to adequately consider sex as a relevant factor in ALS.
Autoimmune Endocrine Dysfunctions Associated with Cancer Immunotherapies
Authors: Ferrari Silvia Martina , Fallahi Poupak , Elia Giusy , Ragusa Francesca , Ruffilli Ilaria , Patrizio Armando , Galdiero Maria Rosaria , Baldini Enke , Ulisse Salvatore , Marone Gianni , Antonelli Alessandro
Published: May 2019

Immune checkpoint inhibitors block the checkpoint molecules. Different types of cancer immune checkpoint inhibitors have been approved recently: CTLA-4 monoclonal antibodies (as ipilimumab); anti-PD-1 monoclonal antibodies (as pembrolizumab and nivolumab); and anti-PD-L1 monoclonal antibodies (as atezolizumab, avelumab, and durmalumab). We collect recent published results about autoimmune endocrine dysfunctions associated with cancer antibody immunotherapies. These agents cause a raised immune response leading to immune-related adverse events (irAEs), varying from mild to fatal, based on the organ system and severity. Immune-related endocrine toxicities are usually irreversible in 50% of cases, and include hypophysitis, thyroid dysfunctions, type 1 diabetes mellitus, and adrenal insufficiency. Anti-PD-1-antibodies are more frequently associated with thyroid dysfunctions (including painless thyroiditis, hypothyroidism, thyrotoxicosis, or thyroid storm), while the most frequent irAE related to anti-CTLA-4-antibodies is hypophysitis. The combination of anti-CTLA-4 and anti-PD-1 antibodies is associated with a 30% chance of irAEs. Symptoms and clinical signs vary depending on the target organ. IrAEs are usually managed by an oncological therapist, but in more challenging circumstances (i.e., for new onset insulin–dependent diabetes, hypoadrenalism, gonadal hormones dysfunctions, or durable hypophysitis) an endocrinologist is needed.
Changes of Thermostability, Organic Solvent, and pH Stability in Geobacillus zalihae HT1 and Its Mutant by Calcium Ion
Authors: Ishak Siti Nor Hasmah , Masomian Malihe , Kamarudin Nor Hafizah Ahmad , Ali Mohd Shukuri Mohamad , Leow Thean Chor , Rahman Raja Noor Zaliha Raja Abd.
Published: May 2019

Thermostable T1 lipase from Geobacillus zalihae has been crystallized using counter-diffusion method under space and Earth conditions. The comparison of the three-dimensional structures from both crystallized proteins show differences in the formation of hydrogen bond and ion interactions. Hydrogen bond and ion interaction are important in the stabilization of protein structure towards extreme temperature and organic solvents. In this study, the differences of hydrogen bond interactions at position Asp43, Thr118, Glu250, and Asn304 and ion interaction at position Glu226 was chosen to imitate space-grown crystal structure, and the impact of these combined interactions in T1 lipase-mutated structure was studied. Using space-grown T1 lipase structure as a reference, subsequent simultaneous mutation D43E, T118N, E226D, E250L, and N304E was performed on recombinant wild-type T1 lipase (wt-HT1) to generate a quintuple mutant term as 5M mutant lipase. This mutant lipase shared similar characteristics to its wild-type in terms of optimal pH and temperature. The stability of mutant 5M lipase improved significantly in acidic and alkaline pH as compared to wt-HT1. 5M lipase was highly stable in organic solvents such as dimethyl sulfoxide (DMSO), methanol, and n-hexane compared to wt-HT1. Both wild-type and mutant lipases were found highly activated in calcium as compared to other metal ions due to the presence of calcium-binding site for thermostability. The presence of calcium prolonged the half-life of mutant 5M and wt-HT1, and at the same time increased their melting temperature (Tm). The melting temperature of 5M and wt-HT1 lipases increased at 8.4 and 12.1 °C, respectively, in the presence of calcium as compared to those without. Calcium enhanced the stability of mutant 5M in 25% (v/v) DMSO, n-hexane, and n-heptane. The lipase activity of wt-HT1 also increased in 25% (v/v) ethanol, methanol, acetonitrile, n-hexane, and n-heptane in the presence of calcium. The current study showed that the accumulation of amino acid substitutions D43E, T118N, E226D, E250L, and N304E produced highly stable T1 mutant when hydrolyzing oil in selected organic solvents such as DMSO, n-hexane, and n-heptane. It is also believed that calcium ion plays important role in regulating lipase thermostability.
Cardiovascular Safety and Possible Benefit of a 5-Alpha Reductase Inhibitor among Benign Prostatic Hyperplasia Patients, A Nationally Representative Cohort of Korean Men
Authors: Chang Jooyoung , Choi Seulggie , Kim Kyuwoong , Park Sang Min
Published: May 2019

Several studies suggest that 5-alpha reductase inhibitors (5ARIs) may be associated with elevated risk of cardiovascular disease (CVD). We investigated the association of 5ARI exposure and CVD incidence using the National Health Insurance Service-Health Screening Cohort, a nationally representative population-based sample of Koreans. We calculated the 4-year cumulative exposure to 5ARI for 215,003 men without prior 5ARI use. Participants were followed from January 1st, 2008 to December 31st, 2015. A subcohort of newly diagnosed benign prostatic hyperplasia (BPH) patients during 2004–2010 was also analyzed. The primary study outcome was CVD and secondary outcomes were myocardial infarction (MI) or stroke. Hazard ratios (HRs) were estimated using Cox proportional hazards models adjusted for conventional risk factors. In both the main cohort and BPH subcohort, the use of any 5ARI did not increase the risk of cardiovascular disease (HR = 1.06; 95% CI = 0.91–1.23; HR = 0.95; 95% CI = 0.88–1.03; respectively). Furthermore, as an unexpected finding, a dose-analysis among the BPH subcohort showed that the highest tertile of 5ARI exposure reduced the risk of CVD (HR = 0.82; 95% CI = 0.72–0.92; p-trend = 0.001), MI (HR = 0.69; 95% CI = 0.50–0.95), and stroke (HR = 0.84; 95% CI = 0.72–0.98) compared to non-users. Among men and BPH patients, 5ARI did not increase the risk of CVD. Among BPH patients, 5ARI use may reduce the risk CVD.
Estimating Directional Data From Network Topology for Improving Tracking Performance
Authors: Tomic Slavisa , Beko Marko , Dinis Rui , Montezuma Paulo
Published: May 2019

This work proposes a novel approach for tracking a moving target in non-line-of-sight (NLOS) environments based on range estimates extracted from received signal strength (RSS) and time of arrival (TOA) measurements. By exploiting the known architecture of reference points to act as an improper antenna array and the range estimates, angle of arrival (AOA) of the signal emitted by the target is first estimated at each reference point. We then show how to take advantage of these angle estimates to convert the problem into a more convenient, polar space, where a linearization of the measurement models is easily achieved. The derived linear model serves as the main building block on top of which prior knowledge acquired during the movement of the target is incorporated by adapting a Kalman filter (KF). The performance of the proposed approach was assessed through computer simulations, which confirmed its effectiveness in combating the negative effect of NLOS bias and superiority in comparison with its naive counterpart, which does not take prior knowledge into consideration.