bluuebunny/crossref_metadata_2025 huggingface dataset:
Can We “Brain-Train” Emotional Intelligence? A Narrative Review on the Features and Approaches Used in Ability EI Training Studies
Authors: Lim Ming D. , Lau Mabel C.
Published: August 2021

Recent studies had demonstrated that specific emotional intelligence (EI) abilities (as measured using the MSCEIT) were related to better performance on cognitive tasks that involved emotional information but not on their non-emotional counterparts. These findings suggest that cognitive control and other executive functioning processes (e.g., working memory) contribute to EI abilities. A well-functioning EI ability is crucial for a number of everyday activities and life outcomes. However, the evidence for training ability EI remains vague as to how these improvements occur. The purpose of this narrative review was to synthesize findings from past EI training research, specifically focusing on their methodology. This was to identify key aspects of the interventions used, to determine the prototypical features between them, as well as to propose a compelling research agenda for future EI training studies. Based on the features found in these studies, we identified two possible approaches in which EI improvements occurred. The first approach was through increasing emotional knowledge and related competencies through teaching and practice. These features were found in the majority of training interventions using a workshop-style training format, reflections, role-plays, and practice with other participants. The second approach used brain-training principles to improve basic cognitive processes, such as executive control or emotional inhibition. Using a cognitive training approach to EI training can provide several advantages, such as allowing researchers to examine EI improvements using the theories of (1) transfer; (2) plasticity; and (3) process-specific changes.
Audio-Guided Mindfulness Meditation During Transcranial Magnetic Stimulation Sessions for the Treatment of Major Depressive Disorder: A Pilot Feasibility Study
Authors: Cavallero Fiamma , Gold Michael C. , Tirrell Eric , Kokdere Fatih , Donachie Nancy , Steinfink Dan , Kriske Joseph , Carpenter Linda L.
Published: August 2021

Background: Mindfulness-Based Cognitive Therapy (MBCT) has been shown to enhance the long-term treatment outcomes for major depressive disorder (MDD), and engagement of specific brain activities during brain stimulation may produce synergistic effects. Audio-guided meditation exercises are a component of MBCT that might be combined with standard transcranial magnetic stimulation (TMS) therapy sessions. We developed and pilot-tested a modified MBCT protocol for patients undergoing a standard course of TMS for MDD.

Methods: Four MBCT audiotracks with differing durations and types of mental focus were selected. Patients listened to the audiotapes through headphones during daily TMS sessions for 5 consecutive weeks. The primary goal was to evaluate the feasibility and acceptability of the meditation intervention with TMS. Changes in self-rated measures of symptom severity, stress, life satisfaction, and mindfulness were also assessed.

Results: Seventeen depressed subjects completed the study and 12 terminated early. Reasons for discontinuation included an inability to meditate in the treatment setting and induction of negative mood states. TMS percussive sensations and clicking sounds hindered the ability of patients to fully concentrate on or hear the voice of the audiotape narrator. Some became overwhelmed or felt increased pressure, anxiety, or aggravation trying to do meditation exercises while receiving TMS.

Conclusion: There is a growing interest in combining TMS with other concurrent psychotherapeutic interventions to optimize treatment outcomes. The results highlight numerous feasibility issues with MBCT via guided audiotapes during TMS treatment. Future work should draw on these shortcomings to evaluate the appropriateness of MBCT for depressed patients undergoing neuromodulation.
Uncertainty and Demand for Insurance: A Theoretical Model of How Self-Control Manages the Optimal Decision-Making
Authors: Adamo Mattia , Malizia Andrea P.
Published: August 2021

With the present work, we aim to mark a beginning line on the study of decision-making of potential consumers in the insurance sector, with the long-term purpose of defining the optimal cognitive processes to be undertaken when deciding whether to purchase insurance or not. Decision-making in conditions of uncertainty is influenced by the dual-self model doers/planner integrated with the hot–cold states and prospect utility function. Thus, we present a theoretical model of choice-making to evaluate the level of optimal self-control necessary to be exerted if the individual is either in the hot or in the cold state depending on the arousal. This theoretical choice-making model lays the ground for the decision journey by following the long-term utility and avoiding gross mistakes that could lead the consumer not to insure, when the odds suggest doing it, or vice versa, in situations when it would not be necessary.
Exploring the Links Between Biophilic and Restorative Qualities of Exterior and Interior Spaces in Leon, Guanajuato, Mexico
Authors: Martínez-Soto Joel , de la Fuente Suárez Luis Alfonso , Ruiz-Correa Salvador
Published: August 2021

The interactive role of the ecological, architectural, biophilic, and sensory qualities of outdoor and indoor spaces in the restorative experiences of urban inhabitants is little known. We analyzed the restorative influence on mood states and situational stress related to exposure to vegetation proportion, spatial extension, landmark salience, biophilic architecture, people density, street visual access, olfactory pleasantness, and noise of 65 public spaces in a Mexican city. The environmental qualities of these places were analyzed with multidimensional scaling (MDS), leading to eight space categories (e.g., historic squares with biophilic architecture, large parks, street scenes, and interiors with non-biophilic architecture). Ratings of the restorative potential, mood states, situational stress, olfactory pleasantness, and noise annoyance were evaluated on such places and modeled through a structural equation modeling (SEM). The model shows that the restorative influence of the environmental qualities on moods and stress was related to a decrease in experiences of negative moods and perceived stress, and an increase of positive mood states. Based on our findings, we discuss design guidelines, emphasizing the relevance of including vegetation and built elements with biophilic qualities to create restorative environments.
A Clinical Rationale for Assessing the Impact of Childhood Sexual Abuse on Adjunctive Subcutaneous Esketamine for Treatment-Resistant Depression
Authors: Magalhães Eduardo Jorge Muniz , Sarin Luciana Maria , Del Sant Lorena Catarina , Lucchese Ana Cecília , Nakahira Carolina , Tuena Marco Aurélio , Puertas Camila Brito , Rodovalho Fava Victor Augusto , Delfino Rodrigo Simonini , Surjan Juliana , Steglich Matheus Souza , Barbosa Matheus Ghossain , Abdo Guilherme , Del Porto José Alberto , Nemeroff Charles B. , Cogo-Moreira Hugo , Lacerda Acioly Luiz Tavares , Mello Andrea Feijo
Published: August 2021

Background: A history of child sexual abuse (CSA) is related to higher suicide rates and poor treatment outcomes in depressed adult patients. Twenty years after the first study investigating the effects of ketamine/esketamine on depression and suicide, there is a lack of data on the CSA effects on this emerging treatment. Here, we assess the impact of CSA on adjunctive subcutaneous (SC) esketamine for treatment-resistant depression (TRD).

Methods: A directed acyclic graphic (DAG) was designed to identify clinical confounders between CSA and esketamine predictors of response. The confounders were applied in a statistical model to predict depression symptom trajectory in a sample of 67 TRD outpatients.

Results: The patient sample had a relatively high prevalence rate of CSA (35.82%). Positive family history of first-degree relatives with alcohol use disorder and sex were clinical mediators of the effects of esketamine in a CSA adult population. Overall, the presence of at least one CSA event was unrelated to esketamine symptom reduction.

Conclusions: Unlike responses to conventional antidepressants and psychotherapy, CSA does not appear to predict poor response to esketamine.
Disability-Adjusted Life Years for the COVID-19 Pandemic in the Mexican Population
Authors: Salinas-Escudero Guillermo , Toledano-Toledano Filiberto , García-Peña Carmen , Parra-Rodríguez Lorena , Granados-García Víctor , Carrillo-Vega María Fernanda
Published: August 2021

Mexico is one of the countries most affected by the COVID-19 disease. Although there is vast information on the disease, there still are unknown data on the societal and economic cost of the pandemic. To estimate this impact, the disability-adjusted life years (DALYs) can be a useful tool.

Objective: To assess the DALYs due to COVID-19 in Mexico.

Methods: We used the data released by the Mexican Ministry of Health to estimate the DALYs by the sum of the years of life lived with disability (YLDs) and the years of life lost (YLLs).

Results: A total of 1,152,885 confirmed cases and 324,570 suspected cases of COVID-19 have been registered. Half of the cases were men, with a median age of 43.4 ± 16.9 years. About 8.3% died. A total of 39,202 YLDs were attributable to COVID-19. The total YLLs caused by COVID-19 were 2,126,222. A total of 2,165,424.5 DALYs for COVID-19 were estimated. The total DALYs were the highest in people between 50 and 59 years. The DALYs for each COVID-19 case were the highest in individuals between 60 and 79 years.

Conclusion: The DALYs generated by the COVID-19 represent a more significant disease burden than that reported for other causes, such as the 2009 H1N1 influenza pandemic. Although it impacts all age groups in terms of disability, the most affected group are people over 50 years of age, whose risk of death is higher.
A Supernumerary Soft Robotic Limb for Reducing Hand-Arm Vibration Syndromes Risks
Authors: Ciullo Andrea S. , Catalano Manuel G. , Bicchi Antonio , Ajoudani Arash
Published: August 2021

The most common causes of the risk of work-related musculoskeletal disorders (WMSD) have been identified as joint overloading, bad postures, and vibrations. In the last two decades, various solutions ranging from human-robot collaborative systems to robotic exoskeletons have been proposed to mitigate them. More recently, a new approach has been proposed with a high potential in this direction: the supernumerary robotic limbs SRLs are additional robotic body parts (e.g., fingers, legs, and arms) that can be worn by the workers, augmenting their natural ability and reducing the risks of injuries. These systems are generally proposed in the literature for their potentiality of augmenting the user’s ability, but here we would like to explore this kind of technology as a new generation of (personal) protective equipment. A supernumerary robotic upper limb, for example, allows for indirectly interacting with hazardous objects like chemical products or vibrating tools. In particular, in this work, we present a supernumerary robotic limbs system to reduce the vibration transmitted along the arms and minimize the load on the upper limb joints. For this purpose, an off-the-shelf wearable gravity compensation system is integrated with a soft robotic hand and a custom damping wrist, designed starting from theoretical considerations on a mass-spring-damper model. The real efficacy of the system was experimentally tested within a simulated industrial work environment, where seven subjects performed a drilling task on two different materials. Experimental analysis was conducted according to the ISO-5349. Results showed a reduction from 40 to 60% of vibration transmission with respect to the traditional hand drilling using the presented SRL system without compromising the time performance.
What Is An Artificial Neural Network And Why Do We Need It?
Authors: Pregowska Agnieszka , Osial Magdalena
Published: August 2021

Your brain controls everything you do, and it is much more powerful than any computer you can find. This complex organ sends messages using cells called neurons, and it never stops analyzing data, even as you sleep. Scientists are trying to understand the brain to create a digital version. But is it possible for computers to do the same things our brains can? For computers to do so, we need to create something called an artificial neural network, which has digital neurons connected into a complex net that resembles the structure of the brain. To make an artificial neural network, we need to use the most universal language—mathematics.
Non-Oscillatory Power Spectrum From States of Low Energy in Kinetically Dominated Early Universes
Authors: Martín-Benito Mercedes , Neves Rita B. , Olmedo Javier
Published: August 2021

Recently, States of Low Energy (SLEs) have been proposed as viable vacuum states of primordial perturbations within Loop Quantum Cosmology (LQC). In this work we investigate the effect of the high curvature region of LQC on the definition of SLEs. Shifting the support of the test function that defines them away from this regime results in primordial power spectra of perturbations closer to those of the so-called Non-oscillatory (NO) vacuum, which is another viable choice of initial conditions previously introduced in the LQC context. Furthermore, through a comparison with the Hadamard-like SLEs, we prove that the NO vacuum is of Hadamard type as well.
Measuring Health-Related Quality of Life in Vietnamese Patients After Kidney Transplantation
Authors: Vu Le Nguyen , Nghia Nguyen Quang , Tuan Tran Minh , Phuong Tran Ha , Vo Hoang-Long , Viet Khai Ninh , Giang Tran Binh
Published: August 2021

Objectives: To consider that the health-related quality of life (HRQOL) has become an inherent part of the patient outcomes in the care and treatment after kidney transplantation (KT). This study aimed to measure HRQOL among a representative sample size of patients after KT by using both the Short Form 36 (SF-36) and the Kidney Disease Quality of Life 36 (KDQOL-36).

Methods and Results: Data of this cross-sectional design were collected in the Organ Transplant Center, Viet Duc University Hospital (Hanoi, Vietnam) from January 2020 to March 2020 and included the patients aged 18 years or over after KT at 6 months, 1 year, and 3 years postoperatively. HRQOL was evaluated through face-to-face interviews by means of the SF-36 and KDQOL-36 measurement tools. According to the SF-36, the overall mean score of HRQOL was 69.13 ± 15.55 and the two domains were the highest scores of “Mental Health” (81.23 ± 14.28) and “General Health” (80.06 ± 14.81). When measuring with the KDQOL-36, the overall mean score was 68.67 ± 13.75 and was the highest in the domain “Symptoms and Problems of Kidney Disease” (87.06 ± 16.00). Both instruments had good reliability for those after KT. The reliability of the SF-36 was high with Cronbach's coefficients α = 0.90. There were positive relationships between the dimensions measured by the KDQOL-36 and SF-36 (correlation coefficient: 0.03–0.69). Similarly, the domains of the SF-36 also had positive correlations with the KDQOL-36 (correlation coefficient: 0.18–0.51). The correlation coefficient between overall HRQOL scores of the SF-36 and KDQOL-36 was 0.62, indicating a strong correlation between the SF-36 and KDQOL-36.

Conclusions: There were slight fluctuations in the HRQOL score in domains in the 3-year follow-up stages, suggesting not having clear change. The mean SF-36 score was consistent with the mean KDQOL-36 score. High reliability and strong correlation were found between two instruments of the SF-36 and KDQOL-36. This study provides the reliability and constructs validity in the combination of two sets of the SF-36 and KDQOL-36 scales for the assessment of HRQOL among post-KT patients, thereby assisting physicians and health professionals in the clinical decision-making, assessment of therapeutic efficacy, and understanding of treatment risk.
The Effectiveness of Self-Instructional Video vs. Classroom Teaching Method on Focused Assessment With Sonography in Trauma Among House Officers in University Hospital
Authors: Isa Mohd Hisham , Lim Kristina , Jaafar Mohd Johar , Mohd Saiboon Ismail
Published: August 2021

Background: The aim of this study was to compare the effectiveness of self-instructional-video (SIV) and classroom training method (CTM) in learning Focus-Assessment with Sonography-in-Trauma (FAST) among house officers (HO).

Method: A randomized controlled study involving house officers working in the university hospital in Malaysia was conducted where participants were randomized into SIV group (intervention) and CTM group (control). Each group had to undergo a 4 h hands-on training. The intervention group has undergone self-training using the video material without any facilitation while the control group received lecture and hands-on training with facilitators. Participants' performance was assessed using a validated Objective Structured Clinical Examination checklist for landmark identification and interpretation of images generated. Learning preference and confidence level were also assessed.

Result: A total of 33 HO were enrolled in this study. Marks obtained in image acquisition by the intervention and control were 25.3 (SD = 5.3) and 25.6 (SD = 2.3) p > 0.05, respectively. While in image interpretation, the mean score for the intervention and control group was 10.3 (SD 1.7) and 9.8 (SD = 1.7) p > 0.05, respectively. Overall performance assessment, showed the intervention group obtained 35.6 (SD = 5.9) compared to control 35.3 (SD = 3.4), p > 0.05. Based on pre-specified determinant these scores difference falls within the 10% of non-inferiority margin. The absolute difference between both groups was 0.3 (CI = −3.75 to 3.21, p = 0.871), which proves non-inferiority but not superiority. In terms of learning preference and confidence to perform FAST, most of the participants preferred the control group approach.

Conclusion: The SIV method is as effective as the CTM for learning FAST among the house officers and served as an alternative to classroom teaching. However, this technique needs improvement in promoting their confidence and preference. Perhaps incorporating a feedback session after going through the SIV would improve the confidence.
Antimicrobial Resistance Profiles and Genes in Streptococcus uberis Associated With Bovine Mastitis in Thailand
Authors: Zhang Tingrui , Niu Guoyi , Boonyayatra Sukolrat , Pichpol Duangporn
Published: August 2021

Streptococcus uberis is recognized as an environmental mastitis pathogen in dairy cattle. The varied success rate of antibiotic treatment for S. uberis intramammary infection may be associated with the antimicrobial resistance (AMR) of these bacteria. This observational study aimed to analyze 228 S. uberis strains associated with bovine mastitis in northern Thailand from 2010 to 2017. AMR and AMR genes were determined by the minimum inhibitory concentration (MIC) using a microdilution method and polymerase chain reaction, respectively. The majority of S. uberis strains were resistant to tetracycline (187/228, 82.02%), followed by ceftiofur (44/228, 19.30%), and erythromycin (19/228, 8.33%). The MIC50 and MIC90 of ceftiofur in 2017 were 2–4-fold higher than those in 2010 (P < 0.01). Resistance to tetracycline and ceftiofur significantly increased between 2010 and 2017 (P < 0.05). The most common gene detected in S. uberis was tetM (199/228, 87.28%), followed by ermB (151/228, 66.23 %) and blaZ (15/228, 6.58 %). The association between tetracycline resistance and tetM detection was statistically significant (P < 0.01). The detection rates of tetM significantly increased, while the detection rates of tetO and ermB significantly decreased during 2010–2017. AMR monitoring for bovine mastitis pathogens, especially S. uberis, is necessary to understand the trend of AMR among mastitis pathogens, which can help create an AMR stewardship program for dairy farms in Thailand.
Folic Acid Absorption Characteristics and Effect on Cecal Microbiota of Laying Hens
Authors: Bai Yan , Wang Rui , Yang Yu , Li Ruirui , Wu Xiaotian
Published: August 2021

This experiment was conducted to investigate the characteristics of folic acid (FA) absorption in laying hens and the effect of FA supplementation on cecal microbiota. A total of 432 healthy hens (30-week-old) were randomly assigned to four diets supplemented with FA: 0, 1, 6, and 24 mg/kg of feed for 8 w. Blood, duodenum, jejunum, ileum, cecum, and cecal chyme samples (six samples per treatment) were collected from the hens at the end of the feeding trial. Expression profiles of folate transport and transformation genes in intestine and cecal microbiota were detected. Results showed that serum folate level significantly increased (P < 0.01) with an increase in dietary FA supplementation, reaching a plateau at 6 mg/kg FA supplementation. The expression of FA transport and transformation genes was not affected in the cecum (P > 0.05) by dietary FA supplementation; however, it was affected in the duodenum, jejunum, and ileum and mostly showed a downward trend in treatment groups (P < 0.05). The genes affected include duodenal folate receptor (Folr) and dihydrofolate reductase (Dhfr), jejunal proton-coupled folate transporter (Pcft) and reduced folate carrier (Rfc), and ileal ATP binding cassette subfamily C member (Abcc2), Abcc3, Rfc, Folr, and Dhfr. Furthermore, according to the operational taxonomic unit classification and taxonomic position identification, the cecal microbiota population of the hens was not affected by dietary FA supplementation at the phylum, class, order, family, genus, and species levels (P > 0.05). However, the relative abundance of some microbiota was affected by dietary FA supplementation (P < 0.05). In conclusion, FA transport from the intestinal lumen into enterocytes, and then into the bloodstream, is strictly regulated, which may be associated with the regulation of the expression profiles of genes involved in FA absorption. Pathogenic bacteria decreased in the cecum, especially at 24 mg/kg supplementation, but the beneficial bacteria (Bifidobacteriaceae) decreased at this level, too. Overall, FA supplementation at 6 mg/kg, which was selected for folate-enriched egg production, did not affect the health and metabolism of laying hens negatively.
Effectiveness of a Video-Based Intervention on Reducing Perceptions of Fear, Loneliness, and Public Stigma Related to COVID-19: A Randomized Controlled Trial
Authors: Valeri Linda , Amsalem Doron , Jankowski Samantha , Susser Ezra , Dixon Lisa
Published: August 2021

Objectives:During the first peak of the COVID-19 outbreak in the United States, we investigated the impact of digital interventions to reduce COVID-19 related fear, loneliness, and public stigma.

Methods:We recruited and randomly assigned 988 United States residents to: 1) no intervention 2) informational sheet to learn about COVID-19, 3) (2) AND video encouraging digital social activity, 4) (2) AND video sensitizing to COVID-19 related stigma (registered in Clinicaltrials.gov). Surveys were conducted between April 2-16, 2020. We employed generalized linear mixed models to investigate intervention effects.

Results:10% of the participants reported not being afraid of people COVID-19+ and 32% reported not feeling lonely. Stigma and fear items reflected acute worries about the outbreak. Relative to the informational sheet only group, video groups led to greater reduction in perceptions of fear towards COVID-19+ (ORvideo.solo = 0.78, p-val<0.001; ORvideo.friend = 0.79, p-val<0.001) and of stigma (BETAvideo.solo = −0.50, p-val<0.001; BETAvideo.friend = −0.69, p-val<0.001).

Conclusion:Video-based interventions lead to reductions in COVID-19-related fear and stigma. No difference in social activity among groups was found, potentially explaining lack of efficacy on loneliness.
Meloxicam and Dexamethasone Administration as Anti-Inflammatory Compounds to Sows Prior to Farrowing Does Not Improve Lactation Performance
Authors: Plush Kate J. , Pluske John R. , Lines David S. , Ralph Cameron R. , Kirkwood Roy N.
Published: August 2021

The aim of this experiment was to determine whether administration of an anti-inflammatory compound to sows prior to farrowing would, via reduced pain and inflammation, increase piglet survival and growth. At day 114 of gestation, multiparous sows were randomly allocated to one of the following treatments: Control (n = 43), which received 10 mL saline, NSAID (n = 55) which received 0.4 mg/kg meloxicam and SAID (n = 54) which received 0.1 mg/kg dexamethasone. Treatments were applied again on day 116 if farrowing had not occurred. There was no treatment effect on piglets born alive or dead from parity two to four sows but in those of parity five and older, NSAID administration reduced the number of piglets born alive and increased the number of piglets born dead (p < 0.05). Sow rectal temperature and incidence of mastitis were unaffected by treatment (p > 0.05). Lactation day two plasma concentrations of cortisol, prostaglandin F2 alpha metabolite and haptoglobin did not differ among treatments (p > 0.05). Treatment effects were not observed in liveborn piglet mortality at any age, or litter weight at day 21 (p > 0.05). Average feed intake during lactation was increased by both NSAID and SAID treatments (p = 0.001). The use of meloxicam prior to farrowing should be avoided as it reduced the number of piglets born alive and did not improve piglet survival and growth.
Influence of Population Income and Climate on Air Pollution in Cities Due to Buildings: The Case of Spain
Authors: Zarco-Soto Fco. Javier , Zarco-Soto Irene M. , Zarco-Periñán Pedro J.
Published: August 2021

Half of the world’s population lives in cities. In addition, more than 40% of greenhouse gas emissions are produced in buildings in the residential and tertiary sectors. Therefore, cities, and in particular their buildings, have a great influence on these emissions. In fact, they are reflected in several of the United Nations’ Sustainable Development Goals. Any measure taken to reach these goals has a significant impact from the point of view of reducing greenhouse gases. An understanding of these goals is the basis of greenhouse gas mitigation. This work analyzed the CO2 emissions from the buildings in cities as a function of the economic income of their inhabitants. For this, databases published by official sources were used. The origins of the CO2 are usually emitted by buildings were analyzed—electrical and thermal, in the form of natural gas. Another variable that influences these emissions is climate. To study only the income variable, the influence of climate has been eliminated. Also, to facilitate analysis, an index has been introduced. As an example of application of the proposed methodology, Spanish cities with more than 50,000 inhabitants were studied. The analysis was carried out by household and by inhabitant. The results showed the following: the higher the income of the citizens, the higher the total and thermal emissions; thermal consumption is elastic, while electrical consumption is inelastic; emissions of electrical origin are almost constant; emissions from electrical energy are greater than those from thermal energy; as income increases, the ratio between emissions of electrical and thermal origin decreases.
Impact of COVID-19 on Mergers, Acquisitions & Corporate Restructurings
Authors: Kooli Chokri , Lock Son Melanie
Published: August 2021

Most economic downturns have stemmed from inefficiencies in the economic system. This research paper aims at investigating the impact of the COVID-19 pandemic—an exogeneous health crisis—on global mergers and acquisition (M&A) activity. By gathering statistical data about global transaction volume, value, and type, the study aims at getting a pulse of how mergers, acquisitions, and other restructuring activities have been utilized to support corporate objectives amidst these unprecedented times. While the full-fledged impact of COVID-19 cannot be fully captured at the moment (early 2021), the study attempts to illustrate how this change to economic stability caused a Schumpeterian creative destruction of industries. As firms prepare for the growth that will follow this downturn, M&A will enable companies to look into a future infused with technology and structurally different business models. This research paper thus captures the deliberate transformation occurring in the deal world to discuss the possible outlook of the M&A deal market in the post-pandemic world.
Can the New and Old Drugs Exert an Immunomodulatory Effect in Acute Myeloid Leukemia?
Authors: Tarantini Francesco , Cumbo Cosimo , Anelli Luisa , Zagaria Antonella , Specchia Giorgina , Musto Pellegrino , Albano Francesco
Published: August 2021

Acute myeloid leukemia (AML) is considered an immune-suppressive neoplasm capable of evading immune surveillance through cellular and environmental players. Increasing knowledge of the immune system (IS) status at diagnosis seems to suggest ever more attention of the crosstalk between the leukemic clone and its immunologic counterpart. During the last years, the advent of novel immunotherapeutic strategies has revealed the importance of immune dysregulation and suppression for leukemia fitness. Considering all these premises, we reviewed the “off-target” effects on the IS of different drugs used in the treatment of AML, focusing on the main advantages of this interaction. The data reported support the idea that a successful therapeutic strategy should consider tailored approaches for performing leukemia eradication by both direct blasts killing and the engagement of the IS.
High Glucose and Advanced Glycation End Products Induce CD147-Mediated MMP Activity in Human Adipocytes
Authors: Mahmoud Abeer M. , Ali Mohamed M.
Published: August 2021

Basigin (CD147) is a transmembrane glycoprotein that regulates several physiological processes, including the production and activity of matrix metalloproteinases (MMPs). The activity of CD147 depends mainly on its glycosylation, which varies among pathophysiological conditions. However, it is unknown whether CD147 activity or its function in MMP regulation are affected by the diabetic environment, which is characterized by high glucose (HG) levels and an excess of glycation end products (AGEs). In this study, we investigated the effect of HG and AGEs on CD147 expression in human adipocytes. We also examined the mediating role of nuclear factor kappa B (NFκB) and receptor of AGE (RAGE) to this effect. Our findings show that carboxymethyl lysine and HG increased CD147 expression and glycosylation, which was accompanied by increases in MMP2 and MMP9 expression and activity, as well as upregulations of the N-acetylglucosaminyltransferase, MGAT5. These effects were abolished by NFκB and RAGE inhibition, CD147 gene silencing, and by the glycosylation inhibitor, tunicamycin. In conclusion, the current findings indicate that AGEs and HG induce CD147 expression and glycosylation in adipocytes, with possible mediation by NFκB and RAGE. One of the critical outcomes of this pathway is augmented MMP activity known to contribute to cardiovascular complications in diabetes.
Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM
Authors: Liu Yunxing , Liang Fang , Dong Zijiong , Li Song , Ye Jianmin , Qin Wei
Published: August 2021

The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of Streptococcus pyogenes Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from S. canis (ScCas9), which has a more relaxed 5′-NNG-3′ PAM, was reported to have activities in human cells and plants. However, the editing ability of ScCas9 has not been tested in zebrafish. Here we characterized and optimized the activity of ScCas9 in zebrafish. Delivered as a ribonucleoprotein complex, ScCas9 can induce mutations in zebrafish. Using the synthetic modified crRNA:tracrRNA duplex instead of in vitro-transcribed single guide RNA, the low activity at some loci were dramatically improved in zebrafish. As far as we know, our work is the first report on the evaluation of ScCas9 in animals. Our work optimized ScCas9 as a new nuclease for targeting relaxed NNG PAMs for zebrafish genome editing, which will further improve genome editing in zebrafish.
miR-135a Suppresses Granulosa Cell Growth by Targeting Tgfbr1 and Ccnd2 during Folliculogenesis in Mice
Authors: Wang Lei , Chen Yaru , Wu Shang , Tang Jinhua , Chen Gaogui , Li Fenge
Published: August 2021

The success of female reproduction relies on high quality oocytes, which is determined by well-organized cooperation between granulosa cells (GCs) and oocytes during folliculogenesis. GC growth plays a crucial role in maintaining follicle development. Herein, miR-135a was identified as a differentially expressed microRNA in pre-ovulatory ovarian follicles between Large White and Chinese Taihu sows detected by Solexa deep sequencing. We found that miR-135a could significantly facilitate the accumulation of cells arrested at the G1/S phase boundary and increase apoptosis. Mechanically, miR-135a suppressed transforming growth factor, beta receptor I (Tgfbr1) and cyclin D2 (Ccnd2) expression by targeting their 3′UTR in GCs. Furthermore, subcellular localization analysis and a chromatin immunoprecipitation-quantitative real-time PCR (ChIP-qPCR) assay demonstrated that the TGFBR1-SMAD3 pathway could enhance Ccnd2 promoter activity and thus upregulate Ccnd2 expression. Finally, estrogen receptor 2 (ESR2) functioned as a transcription factor by directly binding to the miR-135a promoter region and decreasing the transcriptional activity of miR-135a. Taken together, our study reveals a pro-survival mechanism of ESR2/miR-135a/Tgfbr1/Ccnd2 axis for GC growth, and also provides a novel target for the improvement of female fertility.
Disease Progression in CNGA3 and CNGB3 Retinopathy; Characteristics of Slovenian Cohort and Proposed OCT Staging Based on Pooled Data from 126 Patients from 7 Studies
Authors: Tekavčič Pompe Manca , Vrabič Nika , Volk Marija , Meglič Andrej , Jarc-Vidmar Martina , Peterlin Borut , Hawlina Marko , Fakin Ana
Published: August 2021

Achromatopsia has been proposed to be a morphologically predominately stable retinopathy with rare reports of progression of structural changes in the macula. A five-grade system of optical coherence tomography (OCT) features has been used for the classification of structural macular changes. However, their association with age remains questionable. We characterized the Slovenian cohort of 12 patients with pathogenic variants in CNGA3 or CNGB3 who had been followed up with OCT for up to 9 years. Based on observed structural changes in association with age, the following four-stage classification of retinal morphological changes was proposed: (I) preserved inner segment ellipsoid band (Ise), (II) disrupted ISe, (III) ISe loss and (IV) ISe and RPE loss. Data from six previously published studies reporting OCT morphology in CNGA3 and CNGB3 patients were additionally collected, forming the largest CNGA3/CNGB3 cohort to date, comprising 126 patients aged 1–71 years. Multiple regression analysis showed a significant correlation of OCT stage with age (p < 0.001) and no correlation with gene (p > 0.05). The median ages of patients with stages I–IV were 12 years, 23 years, 27 years and 48 years, respectively, and no patient older than 50 years had continuous ISe. Our findings suggest that achromatopsia presents with slowly but steadily progressive structural changes of the macular outer retinal layers. However, whether morphological changes in time follow the proposed four-stage linear pattern needs to be confirmed in a long-term study.
Microstructure and Tribological Properties of Fe-Based Laser Cladding Layer on Nodular Cast Iron for Surface Remanufacturing
Authors: Zhang Dongya , Li Zhongwei , Fan Hongwei , Rui Hongbin , Gao Feng
Published: August 2021

In this study, a cladding layer and nitriding layer were prepared on nodular cast iron, to provide guidance for remanufacturing of nodular cast iron. Their microstructure and composition and the tribological properties under dry and starved lubrication conditions were studied. Meanwhile, the contact stresses at different friction stages were simulated through the finite element method. The micro-hardness of the cladding layer and nitriding layer were 694 HV0.5 and 724.5 HV0.5, which were 4 times and 4.2 times higher than that of the substrate. For dry friction conditions, the wear resistance of the cladding layer and nitriding layer were 113.2 times and 65.5 times that of the substrate. For starved lubrication conditions, the friction coefficients of the cladding layer and nitriding layer were lower than that of the substrate. In addition, their average friction coefficients and wear resistance were gradually reduced with the increase in load. Contact simulation showed that the maximum equivalent stress gradually increased with the friction coefficient during the dry friction, and the peak value of von Mises stress on the nitriding layer was larger than that of the cladding layer, and the nitriding layer was more likely to yield and peel off.
Introduction to Colloidal and Microfluidic Nematic Microstructures
Authors: Čopar Simon , Ravnik Miha , Žumer Slobodan
Published: August 2021

In this brief review, we give an introduction to selected colloidal and microfluidic nematic microstructures, as enabled by the inherent anisotropy and microscopic orientational ordering in complex liquid crystalline materials. We give a brief overview of the mesoscopic theory, for equilibrium and dynamics, of nematic fluids, that provides the framework for understanding, characterization, and even prediction of such microstructures, with particular comment also on the role of topology and topological defects. Three types of nematic microstructures are highlighted: stable or metastable structures in nematic colloids based on spherical colloidal particles, stationary nematic microfluidic structures, and ferromagnetic liquid crystal structures based on magnetic colloidal particles. Finally, this paper is in honor of Noel A. Clark, as one of the world pioneers that helped to shape this field of complex and functional soft matter, contributing at different levels to works of various groups worldwide, including ours.
The Influence of Water/Binder Ratio on the Mechanical Properties of Lime-Based Mortars with White Portland Cement
Authors: Vasovic Dejan , Terzovic Jefto , Kontic Ana , Okrajnov-Bajic Ruza , Sekularac Nenad
Published: August 2021

Protecting the built cultural heritage is one of the most important tasks in architectural practice. The process of repair is time-consuming, weather-dependent, and sensitive to materials applied. Introducing new materials in historic building repair in order to decrease the time needed for repair, brings some risk in the preservation process. The most common material for masonry repair is lime mortar. Adding cement to lime mortar can improve the mechanical properties of mortar and speed up the repair process. The high amount of cement may increase the strength, but decrease ductility and permeability of mortar, causing damages to protected buildings. An increase in strength with the smallest amounts of cement demands optimization of water content in the mixture. Tests were performed to investigate the influence of the water/binder (w/b = water/(lime + cement) ratio on mortar strength and water permeability. An air-entraining agent (AEG) was introduced to improve permeability. Results confirmed that adding small amounts of cement to lime (20% by weight) and decreasing of w/b ratio, improves the strength, with almost negligible influence on water permeability. The addition of very small amounts of AEG did not decrease the strength, nor the permeability.
3D Puzzle in Cube Pattern for Anisotropic/Isotropic Mechanical Control of Structure Fabricated by Metal Additive Manufacturing
Authors: Ikeo Naoko , Fukuda Hidetsugu , Matsugaki Aira , Inoue Toru , Serizawa Ai , Matsuzaka Tadaaki , Ishimoto Takuya , Ozasa Ryosuke , Gokcekaya Ozkan , Nakano Takayoshi
Published: August 2021

Metal additive manufacturing is a powerful tool for providing the desired functional performance through a three-dimensional (3D) structural design. Among the material functions, anisotropic mechanical properties are indispensable for enabling the capabilities of structural materials for living tissues. For biomedical materials to replace bone function, it is necessary to provide an anisotropic mechanical property that mimics that of bones. For desired control of the mechanical performance of the materials, we propose a novel 3D puzzle structure with cube-shaped parts comprising 27 (3 × 3 × 3) unit compartments. We designed and fabricated a Co–Cr–Mo composite structure through spatial control of the positional arrangement of powder/solid parts using the laser powder bed fusion (L-PBF) method. The mechanical function of the fabricated structure can be predicted using the rule of mixtures based on the arrangement pattern of each part. The solid parts in the cubic structure were obtained by melting and solidifying the metal powder with a laser, while the powder parts were obtained through the remaining nonmelted powders inside the structure. This is the first report to achieve an innovative material design that can provide an anisotropic Young’s modulus by arranging the powder and solid parts using additive manufacturing technology.
Mechanism and Effect of the Dilution Gas Flow Rate on Various Fe–Si/SiO2 Soft Magnetic Composites during Fluidised Bed Chemical Vapour Deposition
Authors: Wu Zhaoyang , Gao Zihan , Zhao Qian , Kong Hui , Li Mingyang , Jia Jixiang
Published: August 2021

The effect of the dilution gas flow rate on inorganic oxide insulating layers can improve fluidised bed chemical vapour deposition (FBCVD) in Fe–Si/inorganic-oxide soft magnetic composites and obtain excellent magnetic properties. Herein, Fe–Si/SiO2 composite particles coated via FBCVD and deposited at a 125–350 mL/min Ar-dilution gas flow rate were prepared and sintered into soft magnetic composites. Results demonstrate that SiO2 deposited on the Fe–Si substrate particle surface changed from submicron SiO2 clusters (125 mL/min) to an incomplete SiO2 film, then to a complete SiO2 film, and finally to a porous SiO2 film as the Ar-dilution gas flow rate increased. SiO2 layers began to transform from the amorphous to the beta-cristobalite state with a hexagonal crystal structure between 1149.45 K and 1280.75 K. However, the SiO2 amorphous layers’ crystallisation did not affect the Fe–Si substrate particles’ crystal structure. With the increasing Ar-dilution gas flow rate, the saturation magnetisation of Fe–Si/SiO2 soft magnetic composites initially decreased and then increased. The electrical resistivity increased before 150 mL/min, followed by an increase between 150 and 250 mL/min and then decreased, whereas the total core loss exhibited the opposite trend. These results show that magnetic performance can be promoted by selecting a suitable dilution flow rate.
Copper Oxide Films Deposited by Microwave Assisted Alkaline Chemical Bath
Authors: Galeazzi Isasmendi Reina , Gonzalez Panzo Isidro Juvenal , Morales-Ruiz Crisóforo , Romano Trujillo Román , Rosendo Enrique , García Iván , Coyopol Antonio , García-Salgado Godofredo , Silva-González Rutilo , Oliva Arias Iván , Tabasco Novelo Carolina
Published: August 2021

Copper oxide (CuO) films were deposited onto glass substrates by the microwave assisted chemical bath deposition method, and varying the pH of the solution. The pH range was varied from 11.0 to 13.5, and the effects on the film properties were studied. An analytical study of the precursor solution was proposed to describe and understand the chemical reaction mechanisms that take place in the chemical bath at certain pH to produce the CuO film. A series of experiments were performed by varying the parameters of the analytical model from which the CuO films were obtained. The crystalline structure of the CuO films was studied using X-ray diffraction, while the surface morphology, chemical composition, and optical band-gap energy were analyzed by scanning electron microscopy, X-ray photoelectron spectroscopy, and UV–Vis spectrophotometry, respectively. The CuO films obtained exhibited a monoclinic crystalline phase, nanostructured surface morphology, stoichiometric Cu/O ratio of 50/50 at%, and band-gap energy value of 1.2 eV.
Mires in Europe—Regional Diversity, Condition and Protection
Authors: Tanneberger Franziska , Moen Asbjørn , Barthelmes Alexandra , Lewis Edward , Miles Lera , Sirin Andrey , Tegetmeyer Cosima , Joosten Hans
Published: August 2021

In spite of the worldwide largest proportional loss of mires, Europe is a continent with important mire diversity. This article analyses the condition and protection status of European mire ecosystems. The overview is based on the system of European mire regions, representing regional variety and ecosystem biodiversity. We combined peatland distribution data with land cover maps of the Copernicus Land Monitoring Service as well as with the World Database on Protected Areas to assess the extent of degraded peatlands and the proportion of peatlands located in protected areas in each European mire region. The total proportion of degraded peatlands in Europe is 25%; within the EU it is 50% (120,000 km2). The proportion of degradation clearly increases from north to south, as does the proportion of peatlands located within protected areas. In more than half of Europe’s mire regions, the target of at least 17% of the area located in protected areas is not met with respect to peatlands. Data quality is discussed and the lessons learned from Europe for peatland conservation are presented.
Hyperledger Fabric Access Control System for Internet of Things Layer in Blockchain-Based Applications
Authors: Iftekhar Adnan , Cui Xiaohui , Tao Qi , Zheng Chengliang
Published: August 2021

Blockchain-based applications are gaining traction in various application fields, including supply chain management, health care, and finance. The Internet of Things (IoT) is a critical component of these applications since it allows for data collection from the environment. In this work, we integrate the Hyperledger Fabric blockchain and IoT devices to demonstrate the access control and establish the root of trust for IoT devices. The Hyperledger Fabric is designed to be secure against unwanted access and use through encryption protocols, access restrictions, and cryptography algorithms. An attribute-based access control (ABAC) mechanism was created using Hyperledger Fabric components only to gain access to the IoT device. Single board computers based on the ARM architecture are becoming increasingly powerful and popular in automation applications. In this study, the Raspberry Pi 4 Model B based on ARM64 architecture is used as the IoT device. Because the ARM64 architecture is not supported by default, we build executable binaries and Docker images for the ARM64 architecture, using the Hyperledger Fabric source code. On an IoT device, we run the fabric node in native mode to evaluate the executable binaries generated for the ARM64 architecture. Through effective chaincode execution and testing, we successfully assess the Hyperledger fabric blockchain implementation and access control mechanism on the ARM64 architecture.