bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of the Most Frequent Neuropsychiatric Diagnoses in Hospitalized SARS-CoV-2 Patients Evaluated by Liaison Psychiatry: Cross-Sectional Study
Authors: Fernandes Luis Afonso , Garcia Ribeiro Catarina , Martins Marisa , Figueiredo Inês , Dias Manuel , Facucho João , Carreno Joana , Guerra Inês , Vieira Carlos , Oliveira Catarina , Luís Alice , Maia Teresa
Published: February 2022

Introduction: The SARS-CoV-2 infection has been associated with the acute onset of mental and behavioural symptoms and psychiatric disorders. The aim of this study was to assess the prevalence of the different neuropsychiatric diagnoses in hospitalized patients with SARS-CoV-2 infection assessed by Liaison Psychiatry.Material and Methods: We performed a cross-sectional study in a hospital near Lisbon, Portugal. We reviewed the electronic health records from all inpatients with a positive SARS-CoV-2 RT-PCR test that were assessed by the Liaison Psychiatry Unit (LPU) between February and December 2020. We reviewed relevant sociodemographic and clinical data, including 15 neuropsychiatric symptoms. The prevalence of psychiatric disorders was our main outcome. We also explored differences between two groups: patients with delirium (delirium group) and patients without delirium (no delirium group).Results: We included 46 cases [Age: median = 67 years; interquartile range (IQR) = 24)], with 60.9% male individuals. Delirium was the most frequent diagnosis in our sample (43.5%), followed by major depressive disorder (21.7%). Patients with delirium were more likely to suffer from COVID-19 symptoms (delirium: 19/20, 95%; no delirium: 14/26, 53.8%; p = 0.02), and to have a longer time interval between a positive SARS-CoV-2 RT-PCR test and an evaluation by the LPU (delirium: median = 16.5 days, IQR = 16; no delirium: median = 8 days, IQR = 16.3; p = 0.045). Agitation (52.2%) and cognitive symptoms (47.8%) were the most reported neuropsychiatric symptoms.Conclusion: We found a high prevalence of delirium in our sample. This finding is in line with recent literature concerning hospitalized COVID-19 patients The higher frequency of COVID-19 symptoms found in the delirium group suggests a possible association between symptomatic SARS-CoV-2 infection and delirium onset.
HIGH-RISK INTERNET ADDICTION IN ADOLESCENTS DURING PANDEMIC COVID-19 AND PARENTS’ ROLE
Authors: Putri Ayu , Setiawati Yunias , Shieh Yi-Ting , Lin Sih-Hsien
Published: February 2022

Background: The use of smartphones among adolescents has been increasing during the COVID-19 pandemic, especially using the internet daily, because adolescents do not have physical activities during the lockdown. Using the internet for doing assignments from school, using social media as a substitute for meeting with their friends directly, introducing new people and the internet for entertainment such as playing the game online, listening to music or watching a film. Purpose: This literature aimed to study the risk of internet addiction in adolescents during the COVID-19 pandemic and know parents’ role to accompany adolescents while using the internet daily. Methods: A review of articles on internet use in adolescents during the pandemic was done to determine the risk of internet addiction. This article included article review, systematic review, research journals, qualitative and quantitative methods, and books. Meanwhile, the exclusion journals were not in English. The search of journals accessed from Google Scholar, Pubmed, and Science Direct with keywords ‘adolescents’, ‘internet addiction’, ‘parenting’, ‘education’ and ‘COVID-19’. Results: Internet use has been increasing during the COVID-19 pandemic and is at risk of causing addiction to adolescents. Unattended use of the internet and time restrictions from parents are at risk of causing internet addiction in adolescents. Conclusion: Internet use has been increasing during the COVID-19 pandemic and is at risk of causing addiction in adolescents. Time restriction and parental supervision are needed for adolescents to use the internet daily.
PERFORMANSI EOIP-PPTP DENGAN EOIP-L2TP PADA ROUTER MIKROTIK
Authors: Arifwidodo Bongga
Published: February 2022

Router berfungsi melewatkan paket Internet Protocol (IP) dari suatu jaringan yang berbeda, dengan metode pengalamatan serta protokol tertentu agar bisa melewatkan suatu paket data. Suatu topologi jaringan untuk mengirimkan sebuah data digunakan berbagai protocol seperti PPTP (Point to Point Tunneling Protocol), L2TP (Layer 2 Tunneling Protocol), dan EoIP (Ethernet over Internet Protocol). Dimana Protokol ini digunakan sebagai keamanan data yang akan di kirim maupun diterima. Metode tunneling adalah suatu teknologi yang ditugaskan untuk menangani hingga menyediakan koneksi point to point dari sumber tujuannya. Tunnel ini menggunakan EoIP Tunnel antara PPTP maupun L2TP dalam jaringan untuk memproses pengiriman data, maka dari itu penelitian ini akan membandingkan kinerja PPTP dengan L2TP pada router mikrotik. Pada penelitian ini dilakukan uji coba untuk mengetahui seberapa baik layanan file sharing dengan jaringan PPTP dan L2TP dengan Tunnel EoIP, kemudian dianalisa parameter QoS. Pengujian menggunakan 3 skenario pada layanan file sharing sebesar 5MB, 10MB, dan 15MB. Hasil layanan file sharing PPTP dan L2TP yang termasuk kategori sangat bagus menurut standarisasi TIPHON adalah L2TP. Untuk nilai packet loss yang digunakan pada layanan file sharing L2TP lebih baik dari PPTP. Terlihat nilai packet loss yang didapat pada variasi 15 Mbps sekitar 0 - 1% dibandingkan PPTP dengan packet loss sekitar 17%.
Pemilah dan Penghitung Uang Logam Berdasarkan Diameter Menggunakan Sensor TCRT5000
Authors: Indriyanto Slamet
Published: February 2022

Uang sebagaimana diketahui sebagai alat pembayaran baik barang maupun jasa, memiliki bentuk fisik dalam bentuk kertas dan logam. Uang logam Rupiah biasanya terbuat dari bahan alumunium atau nikel. Setiap kepingnya memiliki tekstur tertentu pada sisi uang untuk membedakan tiap pecahan. Di Indonesia terdapat 4 jenis uang logam yang beredar saati ini, yaitu pecahan Rp. 100, 200, 500 dan 1000 rupiah. Uang logam jika dalam jumlah banyak dan bercampur akan merepotkan jika harus dipisahkan dan dihitung satu demi satu. Untuk itu diperlukan suatu alat yang dapat berfungsi untuk memilah dan sekaligus menghitung jumlah uang logam. Pada jurnal ini telah dirancang dan dibuat suatu alat pemilah dan penghitung uang logam menggunakan sensor TCRT5000. Pemilahan uang logam yang ada berdasarkan diameter dari masing-masing uang logam, dan sensor digunakan untuk menghitung berapa nominal yang sudah tertampung dalam wadah yang disediakan. Hasil pengujian didapatkan dari 100 kali percobaan uang logam Rp.100 rupiah terdapat 2 percobaan tidak terdeteksi oleh sensor. Pengujian uang logam Rp200 rupiah terdapat juga 2 kali percobaan tidak tereteksi oleh sensor. Pengujian uang logam Rp.500 rupiah dan Rp.1000 rupiah tidak ada kegagalan pendeteksian ataupun penyortiran. Jadi, dari pengujian tersebut dapat disimpulkan bahwa tingkat akurasi pendeteksian uang logam Rp.100 dan Rp.200 rupiah sebesar 98%, dan pendeteksian uang logam Rp.500 dan Rp.1000 rupiah memiliki tingkat akurasi sebesar 100%. Dari sisi penyortiran, semua koin yang diuji bisa masuk ke dalam wadah sesuai dengan diameter dan nominal, sehingga tingkat akurasi penyortiran semua uang logam memiliki tingkat akurasi 100%.
Analisis Performansi Jaringan Indihome Fiber Di Purwokerto
Authors: Suryandari Pramusinta Clara
Published: February 2022

FTTH merupakan teknologi jaringan menggunakan kabel serat optik sebagai media transmisinya. Transmisi fiber optik memiliki keunggulan dimana sangat mendukung pengiriman informasi triple play secara lebih efektif. Oleh karena itu PT Telkom sebagai salah satu perusahaan telekomunikasi terbesar di Indonesia telah memanfaatkan arsitektur FTTH untuk mendukung layanan triple play, layanan tersebut dikenal dengan Indihome Fiber. Pada jaringan FTTH sering terjadi gangguan pada khususnya untuk layanan Indihome Fiber. Oleh karena itu, penelitian ini dilakukan dengan perhitungan performansi pada parameter link budget dengan 5 sample pelanggan dan perhitungan availability dengan data SLG (Service Level Guarantee) untuk layanan triple play yaitu voice, video dan data. Layanan voice diperoleh dari data SLG pada telepon, layanan video  diperoleh dari data SLG pada IPTV dan layanan data diperoleh dari data SLG pada internet. Analisis performansi menggunakan metode perhitungan link budget dengan standar ITU-T G.984 dimana sensitivitas  -28 dBm dan availability  batas minimal standar dari PT. Telkom 95%. Hasil perhitungan menunjukkan bahwa daerah Berkoh yang merupakan pelanggan dengan jarak terjauh dari 5 sample perhitungan daya terima diperoleh (-24,014) dBm untuk downlink dan (-28,297) dBm untuk uplink. Perhitungan margin daya didapat hasil 3,986 dBm untuk downlink dan (-0,297) dBm untuk uplink. Berdasarkan perhitungan nilai redaman link budget memenuhi standar, akan tetapi margin daya yang dihasilkan kecil. Hasil availability untuk telepon yang sudah mencapai target sebesar 95,39% dibulan September, availability untuk internet tidak mencapai target, karena banyak gangguan yang disebabkan oleh human error dan availability untuk IPTV sudah mencapai target sebesar 96,72% di bulan Juli dan 97,97% di bulan Agustus.Jaringan FTTH, Triple Play, Link Budget, Availability
Analisis Utilisasi Bandwidth dan Power Pada Modulasi QPSK Transponder Extended C-Band Transmisi Loopback Pada Satelit Telkom 3S
Authors: Nur Faizah Intan Rizqiyani
Published: February 2022

Sistem komunikasi satelit memiliki jangkauan yang sangat luas. Bandwidth dan power merupakan sumber daya yang terbatas dan mahal sehingga diperlukan adanya optimalisasi utilisasi bandwidth dan power, kondisi optimalisasi adalah apabila perbandingan persentase jumlah carrier bandwidth dan power 1:1. Kondisi optimalisasi dipengaruhi  oleh perhitungan link budget, penggunaan modulasi yang tepat dan pengaturan FEC. Teknik modulasi QPSK, dengan FEC yang digunakan adalah ¾, 7/8, dan 0,93 menggunakan antena Hub 9 m dan VSAT SCPC berdiameter 2,4 m dan data rate sebesar 1024 kbps. Berdasarkan hasil penelitian ini didapatkan hasil perbandingan jumlah carrier bandwidth dan jumlah carrier power pada modulasi QPSK yang paling baik untuk digunakan adalah pada FEC 0,93 sekalipun belum mencapai kondisi optimalisasi dengan nilai persentase yang dihasilkan sebesar 65,7 %. Sedangkan, FEC yang paling buruk untuk digunaan adalah FEC ¾ dengan hasil persentase sebesar 52,2 %.

 

Keywords: Satelit Telkom 3S,  Bandwidth, Power, FEC, QPSK.
SKKL PERFORMANCE ANALYSIS OF SUBMARINE CABLE COMMUNICATION SYSTEM (SKKL) EDFA - SOA AMPLIFIER LINK JAVA – BALI USING OPTISYSTEM
Authors: Tarayana Andhara Ersa
Published: February 2022

The Submarine Cable Communication System (SKKL) is a communication backbone that is deployed under the sea that used to connect networks between islands and between countries. DWDM (Dense Wavelength Division Multiplexing) technology can support long distance communication. Repeater used to reduce the impact of loss, on the detector side the signal can be detected properly. EDFA (Erbium Doped Fiber Amplifier) and Semiconductor Optical Amplifier (SOA) amplifiers can minimize the impact of loss and increasing the gain of each amplifier. The EDFA (Erbium Doped Fiber Amplifier) is an optical amplifier that can work at a wavelength of 1550 nm which provides amplification for the input signal that passes through it which is doped with the element erbium (Er). SOA amplifier is an optical amplifier that utilizes cavity or room cavity to strengthen light. By using branching unit configuration and repeaterless, repeatered, and parallel in-line amplifier configuration and optical system software 7.0 with system reliability parameters such as Q-factor, Bit Error Rate, Power Receiver, and Signal to Noise Ratio with power variations (0 dBm, 2 dBm, 4 dBm, 6 dBm, 8 dBm) at a frequency of 100 GHz for 10 channels. The best result on the Q-Factor parameter is 15,928, the BER parameter is 5.87 x 10-057, the receiver power parameter is -10,319 dBm, and the SNR is 42,713 dB. In this research the best value obtained in the Parallel in-line configuration. So as the best average is in the Repeatered EDFA configuration.
Analisis Konfigurasi Repeater-ed dengan Penguat EDFA pada Jaringan Sistem Komunikasi Kabel Laut (SKKL) Link Jawa (Surabay) – Makassar Menggunakan Optisystem
Authors: Masykuroh Kholidiyah , Budi Imam Muhammadi Pradono , Agustias Retno
Published: February 2022

Sistem Komunikasi Kabel Laut (SKKL) digunakan untuk menyediakan koneksi internet antar pulau di Indonesia. Karena jarak jangkauan yang sangat jauh, pada SKKL menggunakan fiber optik dengan menerapkan DWDM. Salah satu konfigurasi penguatan yang digunakan pada SKKL yaitu konﬁgurasi repeater-ed. Penelitian ini akan membahas mengenai performa SKKL pada link Surabaya-Makassar dengan jarak 869 Km. Jenis konﬁgurasi yang diterapkan adalah repeater-ed. Jenis penguat yang digunakan adalah EDFA. Link Surabaya – Makassar dipilih sebagai wilayah penelitian karena padatnya kebutuhan koneksi internetnya. Link ini memiliki. Jarak tempuh yang jauh, semakin jauh jarak tempuh tentu menyebabkan penurunan daya. Pemodelan simulasi dilakukan dengan konfigurasi menggunakan penguat atau dikenal sebagai repeater-ed. Selain itu, simulasi menggunakan 30 kanal dan spasi kanal 80 GHz. Lima variasi Daya Pengirim dengan nilai selisih antar daya sebesar 2 dBm. Kelima variasi Daya Pengirim () tersebut adalah -2 dBm, 0 dBm, 2 dBm, 4 dBm, dan 6 dBm. Parameter yang digunakan meliputi: BER, Q-Factor, Daya Penerima (), dan SNR. Berdasarkan hasil simulasi menunjukan bahwa seluruh kanal memiliki kinerja yang baik karena hasil atau nilai sudah memenuhi bahkan ada beberapa yang mendekati nilai standar yang telah ditentukan. Akan tetapi pada daya 6 dBm mengalami nilai BER yang sangat tinggi. Nilai rata-rata terbaik didapatkan pada variasi Daya Pengirim  = 2 dBm dan  = 4 dBm.
STRATEGI ADAPTASI NELAYAN DI DESA ALUE NAGA DALAM MENGHADAPAI DAMPAK PERUBAHAN IKLIM
Authors: Susanti Elly , Mujiburrahmad Mujiburrahmad , Sahlida Aurum
Published: February 2022

The purpose of this study is to look at the climate change felt by fishermen in Alue Naga Village and identify economic adaptation strategies undertaken by fishermen in Alue Naga Village in dealing with the effects of climate change. The study was conducted by means of observing the observation of activities carried out directly to the location of the study of the research object using the interview method with a questionnaire that was processed using a Likert Scale and New Seven Tools. The results showed that fishermen in Alue Naga Village strongly agree with the perceived climate change that impacts on activities and affects their survival as fishermen who depend their lives on the sea. Fishermen feel climate change and are aware of the impacts caused by interviews through a questionnaire that found that fishermen have not fully adapted to the impacts of climate change. The improvement strategy using the new seven tools produces the improvement strategy by making efforts to foster a culture of understanding climate change that impacts the fishing community’s welfare.
ANALISIS KELAYAKAN USAHATANI PADI PETANI TRANSMIGRAN DI KAMPUNG MARGAMULYA DISTRIK SEMANGGA KABUPATEN MERAUKE PROPINSI PAPUA
Authors: Widyantari Ineke Nursih , Maulany Gerzon Jokomen , Wijayanti Nila
Published: February 2022

Margamulya is one of the villages in the Merauke Regency, which is inhabited by many transmigrant farmers from the Javanese ethnic. They introduce rice farming to local residents in Merauke Regency. The purpose of this research is to figure out the received income, output cost, profitability, and feasibility of rice farming in Margamulya Village, Semangga District, Merauke Regency, Papua. The data used in this study are primary data and secondary data. Primary data was obtained from questionnaires and interviews, while the secondary data was obtained from Central Bureau Statistics of Merauke Regency. The results showed rice farming in Margamulya Village, Semangga District, Merauke Regency is feasible to develop with the received profit is IDR.9,864,480, the total cost is IDR.12,176,495, the profitability of 0.81, and R/C value of 1.81.
PERUBAHAN POLA KONSUMSI RUMAH TANGGA SAAT COVID-19 (Studi Kasus di Kelurahan Drajat, Kota Cirebon, Jawa Barat)
Authors: Bestari Afriliyendra Putri , Noor Trisna Insan
Published: February 2022

Covid-19 is a global pandemic that impacts all sectors. Household is one of the sectors that impacted, and the conditions during the Covid-19 pandemic have been changing especially a decrease in the household consumption expenditure. The purpose of this research is to understand the household food consumption pattern and its relation with the characteristics of respondents. This research used a quantitative descriptive design with a survey method that located in RW 02 Drajat Sub-district, Cirebon City, on October-November 2020. The numbers of research samples are 61 respondents consisting of housewives. The analysis method used is crosstabs method. The results showed that the average age of the respondents was 15-64 years old, the number of family members is 3 persons, the household income per month before and during Covid-19 are IDR 2,000,000-IDR 3,000,000 and < IDR 1,000,000, the education achieved is senior high school or vocational high school, the job is as a housewife, and 62,30% jobs affected due to Covid-19. The household consumption patterns have been changing specifically a decrease in household food consumption expenditure on almost all food commodities except for consumption of vegetable commodities due to the rising prices. However, the amount of food and other non-food consumption tends to remain and decrease. The results showed that the household income and husband’s job have a relation with the household consumption patterns changing, it is indicated by the value of Asymp. Sig. (2-sided) < 0.05, while other variables have no relation with the household consumption patterns changing.
Partisipasi Masyarakat untuk Kesehatan Anak Berbasis Local Wisdom Melalui Rumah Gizi di Desa Kucur Kecamatan Dau Kabupaten Malang
Authors: Anggraheni Ika , Lismanda Yorita Febry
Published: February 2022

Nutrition is an important factor for child development. Parents' knowledge about nutrition plays a role in determining children's nutrition. Parents must determine balanced nutrition for their children. The balance of carbohydrates, protein, vitamins, and other nutrients can increase the child's immunity to disease. The community service team tries to carry out a service program regarding the imbalance between the potential available in an area with the ideal conditions of the community. Local wisdom in the form of corn, cassava, and peanut farming in Kucur Village has not been followed by public awareness about the maximum utilization of local wisdom. The service is carried out with the aim of making guardians of kindergarten students in Kucur Village able to process food that is of interest to children. The target of this program is parents of kindergarten / RA children in Kucur village. The method of implementing the program begins with inculturation, discovery, design, define, monev and reporting of results. As a result of this dedication, the parents have knowledge of their local wisdom so that they can be used to support children's health through nutritious food. Parents have the initiative and are passionate about getting nutritious food for their children
Pemberdayaan Ibu Rumah Tangga Perumahan Bukit Mekarwangi Kota Bogor Pada Masa Pandemi Covid-19 Melalui Program Pelatihan Hidroponik
Authors: Rahmawati Fani Nanda , Alfalathi Siti Aminah
Published: February 2022

Hydroponics has the concept that water is the main ingredient as a substitute for soil to fulfill nutrients in plants. The purpose of empowering housewives is to help overcome food security and reduce kitchen budgets for housewives in Bukit Mekarwangi Housing, Bogor City cereal sub-district during the Covid-19 Pandemic through hydroponic cultivation training so as to gain new insights and farming skills. With the existence of hydroponic cultivation to produce organic plants as food security during the Covid19 period. This training program uses lecture, demonstration and discussion methods. Implementation in this activity includes planning, implementation, and evaluation. The results of the training program indicated that the group of housewives at Bukit Mekarwangi Housing and its surroundings had begun to understand vegetable hydroponic techniques, from equipment preparation, planting to harvesting. The expected impact of these activities is awareness in managing narrow land and utilizing existing tools in the house and making valuable results. So that later they can produce vegetables through hydroponic techniques as a source of food security and also family economic income.
Pelatihan Media Google Classroom Pembelajaran Bahasa dan Sastra Indonesia bagi Kelompok Guru Bahasa Sekolah Menengah Pertama (SMP)
Authors: Dewi Anita Candra , Ma’ruf
Published: February 2022

This activity aims to introduce and provide new understanding to teachers about the importance of using google classroom media in learning Indonesian. The solution offered in this activity is to conduct training for teachers at SMPN 2 Tanete Rilau, Barru Regency, South Sulawesi Province, in the form of understanding the material and applying the google classroom media. The results of the interview show that teachers are still minimal to be creative in teaching online during this Covid-19 pandemic which has almost spread throughout Indonesia. Another result of this training activity is that the training participants consisting of Indonesian language teachers plus teachers from other fields of study have knowledge about the importance of google classroom during the covid 19 pandemic, participants are able to design classes through google classroom, and participants can manage classes during the Covid-19 pandemic. learning process using google classroom.
PENGGUNAAN MEDIA INFORMASI OLEH PENYULUH PERTANIAN BALAI PENGKAJIAN TEKNOLOGI PERTANIAN
Authors: Kustanti Eni , Rusmana Agus , Hadisiwi Purwanti
Published: February 2022

Media komunikasi dan informasi di Kementerian Pertanian RI yang merupakan bagian dari Spektrum Diseminasi Multi Channel tersedia dalam bentuk media cetak, media eletronik, internet, dan media sosial, dapat digunakan oleh penyuluh pertanian sebagai sumber informasi untuk meningkatkan kompetensinya. Penelitian ini bertujuan mengetahui (1)  karakteristik  individu  penyuluh  pertanian di Balai Pengkajian Teknologi Pertanian (BPTP) dan (2) penggunaan media informasi oleh penyuluh pertanian BPTP. Metode penelitian dilakukan secara kuantitatif menggunakan survei online terhadap penyuluh BPTP di 33 provinsi. Hasil penelitian menunjukkan karakteristik individu penyuluh secara usia berada pada usia produktif dan sebanyak 33 % berada pada usia mendekati pensiun, pendidikan di dominasi sarjana (S1), masa kerja penyuluh dominan berada antara 5 sampai 10 tahun dan jabatan fungsional dominan berada pada penyuluh pertanian ahli pertama. Intensitas penggunaan media informasi oleh penyuluh pertanian tergolong rendah (frekuensi kurang dari tiga kali setiap minggu dan durasi kurang dari dua jam per hari). Media sosial dan internet menjadi pilihan penyuluh untuk melakukan akses informasi dengan intensitas (frekuensi dan durasi) lebih tinggi dibandingkan media yang lain. Media internet menjadi pilihan utama oleh penyuluh terkait dengan jenis informasi pertanian yang tersedia maupun kesesuaian isi media dengan kebutuhan informasi penyuluh.
Desain Model Bahan Ajar Berbasis Elektronik dan Minat Belajar Pendidikan Agama Islam Siswa Sekolah Dasar Negeri (SDN) Kelas Bawah di Indonesia
Authors: Nasir Muhammad , Alkhury Saifun Nizar , Rijal Muhammad Khairul
Published: February 2022

Abstract

Religious learning in the era of information technology development, especially in formal education institutions, faces serious challenges, if the teaching materials used are the majority of printed teaching materials and teacher teaching strategies are more dominant in applying a teacher-centered approach. Thus, in this millennial era, teachers are required to be more creative and innovative in developing and designing teaching materials, media, and student-centered learning strategies to increase motivation and interest in learning. This study aims to design an electronic-based Islamic Religious Education (PAI) teaching material model to increase student interest in elementary school (SDN) in Indonesia. Theories related to this design are the characteristics of the content and teaching materials of Islamic Religious Education, constructivist learning theory, technological and humanistic curricula as well as the characteristics of lower grade Public Elementary School (SDN) students. The type of research used is research and development with data collection techniques, interviews, and observations. The research findings show that the results of limited trials and extensive trials of the design of the electronic-based teaching material model of the development results indicate a significant increase in the learning interest of low-grade Public Elementary School (SDN) students in the subject of Islamic Religious Education (PAI) after the treatment. (µ ≤ 0.05).
Change In Metabolic Health Status Over Time And Risk Of Chronic Kidney Disease: A Prospective Study
Authors: Cui Haozhe , Liu Qian , Wu Yuntao , Cao Liying
Published: February 2022

Objective: Metabolically healthy obesity (MHO) is a dynamic condition and is known to increase the risk for chronic kidney disease (CKD). In this study, we aimed to examine the association between metabolic health status and its change over time and CKD risk.Methods: A total of 39463 participants from Kailuan Study were collected body mass index and metabolic health status at 2006/07 and 2010/11 examination. Metabolic abnormality was diagnosed by the presence of any 2 of 4 components (elevated blood pressure, elevated fasting blood glucose, elevated triglyceride and decreased high-density lipoprotein cholesterol). We classified participants into six groups according to metabolic health status and obesity. The changes in obesity and metabolic health status were considered from 2006/07 to 2010/11. Results: Of the participants classified as MHNW or MHO at baseline, 17.25% and 39.64% were classified as MUNW or MUO in 2010/11, respectively. During a mean follow-up of 9.7 years, 5351 participants developed CKD from 2010/11. Compared with participants in the stable MHNW group, the stable MHO group had a significantly higher risk of incident CKD (HR: 1.16; 95% CI: 1.02-1.33), but was lower than that in individuals with MUO. Individuals with metabolic healthy at baseline who changed to metabolic abnormality during follow-up had higher risk of CKD. Conclusions: MHO phenotype or its transition to a metabolically unhealthy phenotype were associated with increased risk of CKD. Stable metabolic health individuals had lower risk of CKD than those with metabolically unhealthy phenotype.
LINE-1 and Alu methylation signatures in autism spectrum disorder and their function in the regulation of autism-related genes
Authors: Saeliw Thanit , Permpoon Tiravut , Iadsee Nutta , Tencomnao Tewin , Sarachana Tewarit , Green Daniel , Sae-Lee Chanachai
Published: February 2022

BackgroundLong interspersed nucleotide element-1 (LINE-1) and Alu elements are retrotransposons whose abilities cause abnormal gene expression and genomic instability. Several studies have focused on DNA methylation profiling of gene regions, but the locus-specific methylation of LINE-1 and Alu elements has not been identified in autism spectrum disorder (ASD). MethodsDNA methylation age was predicted using Horvath’s method. We interrogated locus- and family-specific methylation profiles of LINE-1 and Alu elements (22,352 loci) in ASD blood using publicly-available Illumina Infinium 450K methylation datasets from heterogeneous ASD (n = 52), ASD with 16p11.2 del (n = 7), and ASD with Chromodomain Helicase DNA-binding 8 (CHD8) variants (n = 15). The differentially methylated positions of LINE-1 and Alu elements corresponding to genes were combined with transcriptome data from multiple ASD studies.Results We identified the epigenetic age acceleration significantly decelerated in ASD children over the age of 11 years. We further interrogated locus- and family-specific methylation profiles of LINE-1 and Alu elements (22,352 loci) in ASD blood using publicly-available Illumina Infinium 450K methylation datasets from heterogeneous ASD (n = 52), ASD with 16p11.2 del (n = 7), and ASD with Chromodomain Helicase DNA-binding 8 (CHD8) variants (n = 15). DNA methylation profiling revealed LINE-1 and Alu methylation signatures in each ASD risk loci by which global methylation were notably hypomethylated exclusively in ASD with CHD8 variants. When LINE-1 and Alu elements were clustered into subfamilies, we found methylation changes in a family-specific manner in L1P, L1H, HAL, AluJ, and AluS families in the heterogeneous ASD and ASD with CHD8 variants. Interesting, our results showed that LINE-1 and Alu methylation within target genes is inversely related to the expression level in each ASD variant. Finally, we demonstrate the potential for LINE-1 and Alu methylation signatures to predict ASD individuals from non-ASD. ConclusionsThe DNA methylation signatures of the LINE-1 and Alu elements in ASD, as well as their functional impact on ASD-related genes, have been identified. If confirmed in future larger studies, these finding may contribute to the identification of epigenomic biomarkers of ASD in those with high risk of ASD.
Effects of different LED light spectra on growth and immunity of the Japanese eel (Anguilla japonica) and giant mottled eel (A. marmorata)
Authors: Lin Yen-Ting , Hung Wei-Chun , Yeh Yin-Fu , Lu Kuang-Mao , Cherng Ding-Hwa , Han Yu-San
Published: February 2022

Indoor recirculating aquaculture systems make light control possible and enable the usage of specific coloured lights to promote the growth and immunity of aquaculture species. Five different LED wavelengths (white light, red light [622 nm], green light [517 nm], blue light [467 nm], and dark) were used in this study to evaluate growth and immunity in the glass eel stage of two high-valued anguillid species, Japanese eel ( Anguilla japonica ) and giant mottled eel ( A. marmorata ). There were no significant differences in growth of the Japanese eel among the groups after ­12 weeks of feeding (P > 0.05); the survival rate of each group was over 95%. The giant mottled eel showed better growth in total length and body weight in the red light and dark groups (P < 0.05). Expression levels of immune-related genes were not significantly different between each group of the Japanese eel and the giant mottled eel (P > 0.05). The growth of the Japanese glass eel was not significantly sensitive to different LED wavelengths, while the giant mottled glass eel showed better growth under red light and dark environments. Neither eel species showed significant differences in innate immunity under different LED wavelengths.
A Disease-causing Human Osteoprotegerin Mutant Exists in Hyper-oligomerized Forms
Authors: Luo Yin , Li Miaomiao , Xu Ding
Published: February 2022

Recently, a human mutation of OPG was identified to be associated with familial forms of osteoarthritis. This missense mutation (c.1205A=>T; p.Stop402Leu) occurs on the stop codon of OPG, which results in a 19-residue appendage to the C-terminus (OPG+19). The biochemical consequence of this unusual sequence alteration remains unknown. Here we expressed OPG+19 in 293 cells and the mutant OPG was purified to homogeneity by heparin affinity chromatography and size exclusion chromatography. We found that in sharp contrast to wildtype OPG, which mainly exists in dimeric form, OPG+19 had a strong tendency to form higher-order oligomers. To our surprise, the hyper-oligomerization of OPG+19 had no impact on how it binds cell surface heparan sulfate, how it inhibits RANKL-induced osteoclastogenesis and TRAIL-induced chondrocytes apoptosis. Our data suggest that in biological contexts where OPG is known to play a role, OPG+19 functions equivalently as wildtype OPG. The disease-causing mechanism of OPG+19 likely involves an unknown function of OPG in cartilage homeostasis and mineralization. By demonstrating the biochemical nature of this disease-causing OPG mutant, our study will likely help elucidating the biological roles of OPG in cartilage biology. Running headline: Human osteoprotegerin mutant is highly oligomerized
Past, present, and future trends of overweight and obesity in Belgium using Bayesian age-period-cohort models
Authors: De Pauw Robby , Claessens Manu , Gorasso Vanessa , Drieskens Sabine , Faes Christel , Devleesschauwer Brecht
Published: February 2022

BackgroundConsidering the current overweight and obesity epidemic and its associated increase in non-communicable diseases and healthcare costs, the current study aimed to project the trends in prevalence of overweight and obesity in Belgium using a Bayesian age-period-cohort (APC) model to support policy planning.MethodsHeight and weight of 58,369 adults aged 18+ years, collected in six consecutive cross-sectional health interview surveys between 1997 and 2018, were evaluated. Criteria used for overweight and obesity were defined as body mass index (BMI) ≥ 25, and BMI ≥ 30. A Bayesian APC model was applied to evaluate past trends and associated socio-demographic risk factors, and to forecast trends to 2019-2029. All analyses were performed based on integrated nested Laplace approximation (INLA) and took the complex survey design into account.ResultsThe prevalence of overweight and obesity has increased between 1997 and 2018. If current trends continue, it is likely to that a further increase in the prevalence of overweight and obesity in the population will be seen by 2029 with a probability of growth of 51.2% and 73.3%, respectively. Forecasts indicated a potential prevalence of 50.1% [16.2%; 84.4%] in 2029 for overweight, and 21.4% [9.0%; 43.4%] for obesity. Among survey participants, middle-aged men with no higher education and a middle income showed the highest risk of overweight and obesity.ConclusionsWe projected an alarming increase in the prevalence of overweight and obesity. A decrease in cases seems very unlikely. There is an urgent need to target younger age groups for prevention and implementation of public educational programs to limit the increasing trend in overweight and obesity.
A Slope Test Strategy for Censored Data in Survival Analysis
Authors: Zhang Yumin , Chen Zheng , Hou Yawen
Published: February 2022

Background: Censored data are nearly ubiquitous in survival analysis. If the proportional hazard assumption is invalid, traditional test methods, such as family of log-rank test, lose power rapidly. This paper gives a new slope strategy to improve power of test. The aim of this study is to establish effective tests for censoring data which are familiar in biomedical research and medical practice. Methods: Four tests based on slope are proposed in this paper, and monte carlo simulations with various survival curves, sample size and censoring rates scenarios were performed to evaluate test power. Four common tests, log-rank test, Gehan-Wilcoxon test and Fleming-Harrington with different parameters, were compared with four new slope tests. Results: The slope tests are more effective when the censoring rate is equal to or greater than 30%, especially for scenarios involving early hazard function differences. Two clinical cases also indicate that the proposed slope methods can detect differences for real data. Conclusions: Slope tests may provide a supplement of computing method to survival curve test, it will be more useful considering higher censoring rates.
Human Serum Albumin (HSA) Regulates the Expression of Histone-like Nucleoid Structure Protein (H-NS) in Acinetobacter baumannii
Authors: Escalante Jenny , Nishimura Brent , Tuttobene Marisel R. , Subils Tomás , Pimentel Camila , Georgeos Nardin , Sieira Rodrigo , Bonomo Robert A. , Tolmasky Marcelo E. , Ramírez María Soledad
Published: February 2022

Acinetobacter baumannii, a common cause of nosocomial infection, easily adapts to multiple environments. These characteristics together with this bacterium’s ability to acquire antibiotic resistance determinants make it a notorious pathogen. The presence of human serum albumin (HSA) is associated to modification in levels of expression of numerous genes. The presence of HSA in the culture medium is also correlated with a reduction in levels of the global suppressor histone-like nucleoid structure protein, H-NS. Transcriptome analysis using a wild type and isogenic Δhns strains cultured in lysogeny broth, LB, in the presence or absence of HSA revealed that a subset of eleven, out of 183 genes whose expression is modified by HSA, were regulated through H-NS. Six and five of these genes were up- or down-regulated, respectively. Three of these genes have functions in quorum sensing (acdA, kar and fadD), one in quorum quenching (aidA), two in stress response (katE, ywrO), three in metabolism (phaC, yedL1, and yedL2), one in biofilm formation (csuAB), and one in β-oxidation of fatty acids (fadA). The regulation of these genes was assessed by: i) transcriptional analysis and qPCR at the transcriptional level; and ii) by determining the phenotypic characteristics of each function. The results of these studies support the hypothesis that HSA-mediated reduction of H-NS levels may be one very important regulatory circuit utilized by A. baumannii to adapt to selected environments, such as those where HSA-containing human fluids are abundant.
Is It Sufficient to Evaluate Bone Metastasis Involvement in Breast Cancer using SPECT/CT? A New Approach of SPECT/CT-Guided Targeted Bone Marrow Biopsy
Authors: Li Xiaomin , An Caixia , Zhang Wanchun
Published: February 2022

Objective To investigate the feasibility, safety, and clinical application value of single photon emission computed tomography/computed tomography (SPECT/CT)-guided bone marrow biopsy (BMB) in breast cancer (BC) patients with suspected bone metastases (BM) and compare its diagnostic performance for detection of BM with SPECT/CT. Methods The records of breast cancer patients referred for bone scintigraphy (BS), SPECT/CT and SPECT/CT-guided BMB from January of 2018 to June of 2021 in our hospital were retrospectively reviewed. 49 Patients were consecutively included in this study, all 49 specimens were analyzed by pathological and immunohistochemical studies.The biopsy success rate, total examination time, biopsy operation time, complications, CT radiation dose, and pathological and immunohistochemical results were recorded. The diagnostic performance based on SPECT/CT and SPECT/CT-guided BMB were compared with pathological, immunohis to chemical examinations and the results of subsequent follow-up. Results Bone samples of the sites with high uptake were obtained in all 49 patients under BMB. No severe postoperative complications occurred. Among all 49 cases, 34 specimens were positive for metastatic breast cancer (69%, 34/49), and positive for benign tissue in 15 cases (31%, 15/49). 1 case of 15 cases was subsequently diagnosed as metastatic breast cancer according to the follow-up result. SPECT/CT-guided BMB demonstrated significantly higher negative predictive value (NPV) when compared to SPECT/CT (p=0.021<0.05). Patients with differential expression of ER, PR, and HER-2 between primary lesions and metastatic lesions accounted for 12, 17, and 5 cases, respectively, and the inconsistency rates were 35.2% (12/34), 50% (17/34), and 14.7% (5/34), respectively. Molecular subtype changes occurred in 7 patients, accounting for 47% (16/34) of metastatic patients. Conclusion It is not insufficient to evaluate BM in BC patients using 99mTC-MDP SPECT/CT imaging. SPECT/CT-guided BMB was safe, accurate, and feasible and provided significantly higher sensitivity and NPV when compared to SPECT/CT for detection of BM in BC patients. In addition, our study initially showed the integrated approach that includes SPECT /CT and SPECT/CT-guided BMB, which can obtain one-stop imaging, pathological and molecular typing diagnosis in nuclear medicine department and offer important opportunities for precision treatment to improve quality of life of BC patients with BM.
Monitoring The After-Effects Of Ischemic Stroke Through EEG Microstates And Machine Learning
Authors: Wang Fang , Tian Yu-Chu , Zhang Xueying , Hu Fengyun
Published: February 2022

Background and Purpose: Stroke may cause extensive after-effects such as motor function impairments and disorder of consciousness (DoC). Detecting these after-effects of stroke and monitoring their changes are challenging jobs currently undertaken via traditional clinical examinations. These behavioural examinations often take a great deal of manpower and time, thus consuming significant resources. Computer-aided examinations of the electroencephalogram (EEG) microstates derived from bedside EEG monitoring may provide an alternative way to assist medical practitioners in a quick assessment of the after-effects of stroke. Methods: In this study, we designed a framework to extract microstates maps and calculate their statistical parameters to input to classifiers to identify DoC in ischemic stroke patients automatically. As the dataset is imbalanced with the minority of patients being DoC, an ensemble of support vector machines (EOSVM) is designed to solve the problem that classifiers always tend to be the majority classes in the classification on an imbalanced dataset.Results: The experimental results show EOSVM get better performance (with accuracy and F1-Score both higher than 89%), improving sensitivity the most, from lower than 60% (SVM and AdaBoost) to higher than 80%. This highlighted the usefulness of the EOSVM-aided DoC detection based on microstates parameters. ﻿Conclusion: Therefore, the classifier EOSVM classification based on features of EEG microstates is helpful to medical practitioners in DoC detection with saved resources that would otherwise be consumed in traditional clinic checks.
Influence of Moisture Content, Temperature, and Time on Free Fatty Acid in Stored Crude Palm Oil
Authors: Emebu Samuel , Osaikhuiwuomwan Omokaro , Mankonen Aleksi , Udoye Chinweike , Okieimen Charity , Janáčová Dagmar
Published: February 2022

Consequent to the importance of crude palm oil (CPO) to global food processing industries, and the need for quality assurance of CPO. A kinetic model that describes changes of free fatty acid (FFA) in industrially stored CPO has been developed. CPO FFA is a well-known indicator of the deterioration of CPO. The effect of initial moisture content, storage temperature, and time on CPO FFA has been investigated in this work. Specifically, statistical multi-regression models for changes in FFA and moisture content (MC) were developed at P-value < 0.05 or 95% confidence interval fence. It was found that CPO FFA increases with an increase in moisture content, temperature, and time in their linear term and in respect to decreases in their quadratic term, and interaction between moisture content and temperature. The CPO MC was also found to decrease with an increase in temperature and time and increases in the quadratic term of temperature. Although while the model for CPO FFA, based on Fisher's F-test: \({\text{F}}_{\text{m}\text{o}\text{d}\text{e}\text{l}}\left(6.80\right)<{\text{F}}_{95\text{\%}}\left(19.30\right)\), showed no lack-of-fit; that of CPO MC showed lack-of-fit, \({\text{F}}_{\text{m}\text{o}\text{d}\text{e}\text{l}}\left(13.67\right)\nless {\text{F}}_{95\text{\%}}\left(4.39\right)\). Furthermore, based on inference from the statistical model, their kinetic models were also developed. While the CPO FFA kinetic, found to be a half-order kinetic model and its other auxiliary models showed a very good fit (R2 {0.9933–0.8614} & RMSE {0.0020–3.6716}); that of CPO MC was a poorly fitted first-order kinetic model (R2 {0.9885– 0.3935} & RMSE {0.0605– 17.8501}).
Viable Closed-Loop Supply Chain Network with Considering Robustness and Risk as a Circular Economy
Authors: Lotfi Reza , Nazarpour Hossein , Gharehbaghi Alireza , Sarkhosh Seyyed Mahdi Hosseini , Khanbaba Amirhossein
Published: February 2022

The Viable Closed-loop Supply Chain Network (VCLSCND) is a new concept that integrates sustainability, resiliency, and agility in a circular economy. We suggest a new form of robust stochastic optimization for this problem by minimizing the weighted expected, maximum and Entropic Value at Risk (EVaR). We proposed this form to increase robustness against demand disruption and energy productivity. Finally, we located CLSC components and assigned flow in the automotive industry. The results show that the cost of VCLSCND is less than without viable and has -0.44% gap. By increasing the conservative coefficient and confidence level, decreasing the allowed maximum energy and increasing the scale of the main model, the cost function, time solution and energy consumption grow. We suggested applying the Fix-and-Optimize algorithm for producing upper bound of large-scale. As can be seen, the gap between this algorithm and the main problem for cost, energy and time solution is approximately 6.10%, -8.28%, and 75.01%.
Seed priming with selenium and zinc nanoparticles modifies germination, growth and yield of direct-seeded rice (Oryza sativa L.)
Authors: Adhikary Saju , Biswas Benukar , Chakraborty Debashis , Timsina Jagadish , Pal Srikumar , Banerjee Saon , Hossain Akbar , Roy Sovan , Tarafdar Jagadish Chandra
Published: February 2022

Direct-seeded rice (DSR) seeds are often exposed to multiple environmental stresses in the field, leading to poor emergence, growth and productivity. Appropriate seed priming agents may help to overcome the challenges by ensuring uniform seed germination, and better seedling stand establishment. To examine the effectiveness of sodium selenite, sodium selenate, and zinc oxide nanoparticles (ZnO-NPs) as priming agents for DSR seeds, a controlled pot experiment followed by a field experiment over two consecutive years was conducted on a sandy clay loam soil (Inceptisol) in West Bengal, India. Priming with combinations of all priming agents had advantages over the hydro-priming treatment (control). All the combinations resulted in the early emergence of seedlings with improved vigour. In the field experiment, the combinations of all priming agents increased the plant chlorophyll, phenol and protein contents, leaf area index and duration, crop growth rate, uptake of nutrients (N, P, K, B, Zn and Si) and yield of DSR over the control. Our findings suggest that seed priming with the combination of ZnO-NPs, Na-selenite, and selenate could be a viable option for the sustainable intensification of DSR.