bluuebunny/crossref_metadata_2025 huggingface dataset:
Precision Segmentation of Subretinal Fluids in OCT Using Multiscale Attention-Based U-Net Architecture
Authors: Karn Prakash Kumar , Abdulla Waleed H.
Published: October 2024

This paper presents a deep-learning architecture for segmenting retinal fluids in patients with Diabetic Macular Oedema (DME) and Age-related Macular Degeneration (AMD). Accurate segmentation of multiple fluid types is critical for diagnosis and treatment planning, but existing techniques often struggle with precision. We propose an encoder–decoder network inspired by U-Net, processing enhanced OCT images and their edge maps. The encoder incorporates Residual and Inception modules with an autoencoder-based multiscale attention mechanism to extract detailed features. Our method shows superior performance across several datasets. On the RETOUCH dataset, the network achieved F1 Scores of 0.82 for intraretinal fluid (IRF), 0.93 for subretinal fluid (SRF), and 0.94 for pigment epithelial detachment (PED). The model also performed well on the OPTIMA and DUKE datasets, demonstrating high precision, recall, and F1 Scores. This architecture significantly enhances segmentation accuracy and edge precision, offering a valuable tool for diagnosing and managing retinal diseases. Its integration of dual-input processing, multiscale attention, and advanced encoder modules highlights its potential to improve clinical outcomes and advance retinal disease treatment.
Enhancing Bone Formation Through bFGF-Loaded Mesenchymal Stromal Cell Spheroids During Fracture Healing in Mice
Authors: Takeda Kugo , Saito Hiroki , Shoji Shintaro , Sekiguchi Hiroyuki , Matsumoto Mitsuyoshi , Ujihira Masanobu , Miyagi Masayuki , Inoue Gen , Takaso Masashi , Uchida Kentaro
Published: October 2024

This study aimed to evaluate the osteogenic potential of mesenchymal stromal cell (MSC) spheroids combined with the basic fibroblast growth factor (bFGF) in a mouse femur fracture model. To begin, MSC spheroids were generated, and the expression of key trophic factors (bFGF Bmp2, and Vegfa) was assessed using quantitative PCR (qPCR). A binding assay confirmed the interaction between the bFGF and the spheroids’ extracellular matrix. The spheroid cultures significantly upregulated bFGF, Bmp2, and Vegfa expression compared to the monolayers (p < 0.001), and the binding assay demonstrated effective bFGF binding to the MSC spheroids. Following these in vitro assessments, the mice were divided into five groups for the in vivo study: (1) no treatment (control), (2) spheroids alone, (3) bFGF alone, (4) bFGF-loaded spheroids (bFGF-spheroids), and (5) non-viable (frozen) bFGF-loaded spheroids (bFGF-dSpheroids). Bone formation was analyzed by a micro-CT, measuring the bone volume (BV) and bone mineral content (BMC) of the mice four weeks post-fracture. A high dose of the bFGF (10 µg) significantly promoted bone formation regardless of the presence of spheroids, as evidenced by the increases in BV (bFGF, p = 0.010; bFGF-spheroids, p = 0.006; bFGF-dSpheroids, p = 0.032) and BMC (bFGF, p = 0.023; bFGF-spheroids, p = 0.004; bFGF-dSpheroids, p = 0.014), compared to the controls. In contrast, a low dose of the bFGF (1 µg) combined with the MSC spheroids significantly increased BV and BMC compared to the control (BV, p = 0.012; BMC, p = 0.015), bFGF alone (BV, p = 0.012; BMC, p = 0.008), and spheroid (BV, p < 0.001; BMC, p < 0.001) groups. A low dose of the bFGF alone did not significantly promote bone formation (p > 0.05). The non-viable (frozen) spheroids loaded with a low dose of the bFGF resulted in a higher BV and BMC compared to the spheroids alone (BV, p = 0.003; BMC, p = 0.017), though the effect was less pronounced than in the viable spheroids. These findings demonstrate the synergistic effect of the bFGF and MSC spheroids on bone regeneration. The increased expression of the BMP-2 and VEGF observed in the initial experiments, coupled with the enhanced bone formation in vivo, highlight the therapeutic potential of this combination. Future studies will aim to elucidate the underlying molecular mechanisms and assess the long-term outcomes for bone repair strategies.
The Emerging Role of Immunoglobulins and Complement in the Stimulation of Neuronal Activity and Repair: Not as Simple as We Thought
Authors: Veremeyko Tatyana , Barteneva Natasha S. , Vorobyev Ivan , Ponomarev Eugene D.
Published: October 2024

Neurologic disorders such as traumatic brain injury, multiple sclerosis, Alzheimer’s disease, and drug-resistant epilepsy have a high socioeconomic impact around the world. Current therapies for these disorders are often not effective. This creates a demand for the development of new therapeutic approaches to treat these disorders. Recent data suggest that autoreactive naturally occurring immunoglobulins produced by subsets of B cells, called B1 B cells, combined with complement, are actively involved in the processes of restoration of neuronal functions during pathological conditions and remyelination. The focus of this review is to discuss the possibility of creating specific therapeutic antibodies that can activate and fix complement to enhance neuronal survival and promote central nervous system repair after injuries associated with many types of neurodegenerative diseases.
High-Definition Trans-Spinal Current Stimulation Improves Balance and Somatosensory Control: A Randomised, Placebo-Controlled Trial
Authors: Steingräber Teni , Grönheim Leon von , Klemm Michel , Straub Jan , Sasse Lea , Veldema Jitka
Published: October 2024

Objectives: To investigate and compare the effects of three different high-definition (HD) non-invasive current stimulation (NICS) protocols on the spinal cord on support balance and somatosensory abilities in healthy young people. Methods: Fifty-eight students were enrolled in this crossover study. All participants underwent application of (i) 1.5 mA anodal high-definition trans spinal direct current stimulation (HD-tsDCS), (ii) 1.5 mA cathodal HD-tsDCS, (iii) 1.5 mA high-definition trans spinal alternating current stimulation (HD-tsACS), and (iv) sham HD-tsDCS/ACS over the eighth thoracic vertebra in a randomised order. Balance (Y Balance test), deep sensitivity (Tuning Fork Test), and superficial sensitivity (Monofilament Test) of the lower limbs were tested immediately before and after each intervention. Results: Balance ability improved significantly following anodal HD-tsDCS and HD-tsACS compared with that following sham HD-tsDCS/ACS. Similarly, deep sensitivity increased significantly with anodal HD-tsDCS and HD-tsACS compared to that with sham HD-tsDCS/ACS and cathodal HD-tsDCS. Furthermore, superficial sensitivity improved significantly following anodal HD-tsDCS compared with that after HD-tsACS and cathodal HD-tsDCS. Conclusions: Our data show that HD-tsNICS effectively modulates the balance and somatosensory control of the lower limbs. Several diseases are associated with illness-induced changes in the spinal network in parallel with sensorimotor disabilities. Non-invasive spinal modulation may be a favourable alternative to conventional brain applications in rehabilitation. Future studies should therefore investigate these promising approaches among cohorts of patients with disabilities.
Exploring the Mechanisms and Preventive Strategies for the Progression from Idiopathic Pulmonary Fibrosis to Lung Cancer: Insights from Transcriptomics and Genetic Factors
Authors: Xie Kai , Tan Xiaoyan , Chen Zhe , Yao Yu , Luo Jing , Ma Haitao , Feng Yu , Jiang Wei
Published: October 2024

Background: Idiopathic pulmonary fibrosis (IPF) leads to excessive fibrous tissue in the lungs, increasing the risk of lung cancer (LC) due to heightened fibroblast activity. Advances in nucleotide point mutation studies offer insights into fibrosis-to-cancer transitions. Methods: A two-sample Mendelian randomization (TSMR) approach was used to explore the causal relationship between IPF and LC. A weighted gene co-expression network analysis (WGCNA) identified shared gene modules related to immunogenic cell death (ICD) from transcriptomic datasets. Machine learning selected key genes, and a multi-layer perceptron (MLP) model was developed for IPF prediction and diagnosis. SMR and PheWAS were used to assess the expression of key genes concerning IPF risk. The impact of core genes on immune cells in the IPF microenvironment was explored, and in vivo experiments were conducted to examine the progression from IPF to LC. Results: The TSMR approach indicated a genetic predisposition for IPF progressing to LC. The predictive model, which includes eight ICD key genes, demonstrated a strong predictive capability (AUC = 0.839). The SMR analysis revealed that the elevated expression of MS4A4A was associated with an increased risk of IPF (OR = 1.275, 95% CI: 1.029–1.579; p = 0.026). The PheWAS did not identify any significant traits linked to MS4A4A expression. The rs9265808 locus in MS4A4A was identified as a susceptibility site for the progression of IPF to LC, with mutations potentially reprogramming lung neutrophils and increasing the LC risk. In vivo studies suggested MS4A4A as a promising therapeutic target. Conclusions: A causal link between IPF and LC was established, an effective prediction model was developed, and MS4A4A was highlighted as a therapeutic target to prevent IPF from progressing to LC.
Pheochromocytoma–Paraganglioma Syndrome: A Multiform Disease with Different Genotype and Phenotype Features
Authors: Giacché Mara , Tacchetti Maria Chiara , Agabiti-Rosei Claudia , Torlone Francesco , Bandera Francesco , Izzi Claudia , Agabiti-Rosei Enrico
Published: October 2024

Pheochromocytoma and paraganglioma (PPGL) are rare tumors derived from the adrenal medulla and extra-adrenal chromaffin cells. Diagnosis is often challenging due to the great variability in clinical presentation; the complexity of management due to the dangerous effects of catecholamine excess and the potentially malignant behavior require in-depth knowledge of the pathology and multidisciplinary management. Nowadays, diagnostic ability has certainly improved and guidelines and consensus documents for treatment and follow-up are available. A major impulse to the development of this knowledge has come from the new findings on the genetic and molecular characteristics of PPGLs. Germline mutation in susceptibility genes is detected in 40% of subjects, with a mutation frequency of 10–12% also in patients with sporadic presentation and genetic testing should be incorporated within clinical care. PPGL susceptibility genes include “old genes” associated with Neurofibromatosis type 1 (NF1 gene), Von Hippel Lindau syndrome (VHL gene) and Multiple Endocrine Neoplasia type 2 syndrome (RET gene), the family of SDHx genes (SDHA, SDHB, SDHC, SDHD, SDHAF2), and genes less frequently involved such as TMEM, MAX, and FH. Each gene has a different risk of relapse, malignancy, and other organ involvement; for mutation carriers, affected or asymptomatic, it is possible to define a tailored long-life surveillance program according to the gene involved. In addition, molecular characterization of the tumor has allowed the identification of somatic mutations in other driver genes, bringing to 70% the PPGLs for which we know the mechanisms of tumorigenesis. This has expanded the catalog of tumor driver genes, which are identifiable in up to 70% of patients Integrated genomic and transcriptomic data over the last 10 years have revealed three distinct major molecular signatures, triggered by pathogenic variants in susceptibility genes and characterized by the activation of a specific oncogenic signaling: the pseudo hypoxic, the kinase, and the Wnt signaling pathways. These molecular clusters show a different biochemical phenotype and clinical behavior; they may also represent the prerequisite for implementing customized therapy and follow-up.
Electrochemical Analysis of Amyloid Plaques and ApoE4 with Chitosan-Coated Gold Nanostars for Alzheimer’s Detection
Authors: Shin Min-Kyung , Schuck Ariadna , Kang Minhee , Kim Yong-Sang
Published: October 2024

Monitoring the progression of Alzheimer’s disease (AD) is crucial for mitigating dementia symptoms, alleviating pain, and improving mobility. Traditionally, AD biomarkers like amyloid plaques are predominantly identified in cerebrospinal fluid (CSF) due to their concentrated presence. However, detecting these markers in blood is hindered by the blood–brain barrier (BBB), resulting in lower concentrations. To address this challenge and identify pertinent AD biomarkers—specifically amyloid plaques and apolipoprotein E4 (ApoE4)—in blood plasma, we propose an innovative approach. This involves enhancing a screen-printed carbon electrode (SPCE) with an immobilization matrix comprising gold nanostars (AuNSs) coated with chitosan. Morphological and electrical analyses confirmed superior dispersion and conductivity with 0.5% chitosan, supported by UV–Vis spectroscopy, cyclic voltammetry, and Nyquist plots. Subsequent clinical assays measured electrical responses to quantify amyloid-β 42 (Aβ42) (15.63–1000 pg/mL) and APoE4 levels (0.41 to 40 ng/mL) in human blood plasma samples. Differential pulse voltammetry (DPV) responses exhibited peak currents proportional to biomarker concentrations, demonstrating high linear correlations (0.985 for Aβ42 and 0.919 for APoE4) with minimal error bars. Cross-reactivity tests with mixed solutions of amyloid-β 40 (Aβ40), Aβ42, and ApoE4 indicated minimal interference between biomarkers (<3% variation), further confirming the high specificity of the developed sensor. Validation studies demonstrated a strong concurrence with the gold-standard enzyme-linked immunosorbent assay (ELISA), while interference tests indicated a minimal variation in peak currents. This improved device presents promising potential as a point-of-care system, offering a less invasive, cost-effective, and simplified approach to detecting and tracking the progression of AD. The substantial surface binding area further supports the efficacy of our method, offering a promising avenue for advancing AD diagnostics.
Optimization of Advanced Support Parameters of Surrounding Rock in Tunnels Constructed by ADECO-RS
Authors: Huang Xin , Zhang Huaxu , Guo Jiaqi , Zhang Zonglei , Chao Xiaohui
Published: October 2024

So as to efficiently address the distortion of surrounding rock in tunnels constructed utilizing ADECO-RS, it is crucial to define suitable parameters for advanced support systems. This study took the 8 # tunnel in the F3 portion of the E60 Expressway in Georgia as an engineering case. Initially, the original support scheme underwent systematic monitoring and analysis in the field. Subsequently, the FLAC3D 6.0 software was employed to examine the influence of the advanced pipe roof and tunnel face fiberglass bolts on the steady state of the surrounding rock. Optimization of the support parameters was also proposed. Further, the sensitivity of different parameters to the distortion of the rock surrounding the tunnel was analyzed and ranked via an orthogonal experiment. Ultimately, the effectiveness of the optimization scheme was evaluated by numerical methods and field observations. The findings of the research indicate the following: (1) The monitoring results of the original support parameters show that the irrational design of the support parameters can bring about deformation non-convergence in the tunnel’s surrounding rock. Support parameters must be optimized. (2) The spacing of the pipe roof is positively correlated with the distortion of the surrounding rock. In contrast, the length and the grouting strength are negatively correlated with the distortion of the surrounding rock. The reinforcement density, length, and lap length of glass fiber bolts exhibit an inverse relationship with the distortion of the surrounding rock. (3) The efficacy of pipe shed grouting in mitigating subsidence and deformation of the vault is superior, followed by the spacing of the supports. In contrast, the length of the supports demonstrates comparatively lesser effectiveness. Under optimal parameters, the vault subsidence was reduced by 23.2%, 10.2%, and 2.0%, respectively. The most significant factor controlling the extrusion deformation of the tunnel face is bolt lap length, followed by reinforcement density and then reinforcement length. Extrusion displacement was reduced by 52.5%, 40.3%, and 9.3%, respectively, under the optimal parameters. (4) In comparison to the primordial support system, the optimized support scheme reduces the subsidence of the vault by about one time and the convergence deformation around the cave by about two times. The research findings offer guidance for analogous engineering support design and parameter optimization.
Needs of Deaf People Using Elevators: Identification of Accessibility and Safety Requirements
Authors: Trabucco Dario , Perrucci Giovanni , Giacomello Elena , Costa Marco
Published: October 2024

As urbanization and population growth continue, cities concentrate an increasing amount of people, energy, and economy. Multi-story buildings enable densification, requiring vertical transport for access to upper floors. This is crucial for people with disabilities, who may face barriers in the built environment. Elevators are essential for accessibility, allowing everyone, including people with disabilities, to comfortably access multi-story buildings. However, barriers to inclusivity remain, often subtle and hard to define. This paper highlights one such example, focusing on elevator use by individuals with varying degrees of hearing loss. Currently, they cannot establish one-to-one communication with the outside world if trapped in an elevator. Under EN standards, this issue stems from alarm system requirements that lack effective alternatives to voice communication. Based on this evidence, the research was carried out in two steps, with the aim of understanding the needs of deaf individuals when using elevators by directly involving them in the study. First, a questionnaire conducted in Italy collected information regarding the safety and usability of elevators. Second, a test campaign involving both deaf and normal-hearing participants was carried out to quantify the severity of the issue and evaluate potential solutions to address the identified challenges. The conclusions indicate that current alarm systems in elevators are inadequate for individuals with hearing impairments, and effective alternatives must be implemented.
Decompensated MASH-Cirrhosis Model by Acute and Toxic Effects of Phenobarbital
Authors: Kraus Nico , Uschner Frank Erhard , Moeslein Magnus , Schierwagen Robert , Gu Wenyi , Brol Maximilian Joseph , Fürst Eike , Grünewald Inga , Lotersztajn Sophie , Rautou Pierre-Emmanuel , Duran-Güell Marta , Flores Costa Roger , Clària Joan , Trebicka Jonel , Klein Sabine
Published: October 2024

Metabolic dysfunction-associated Steatohepatitis (MASH), is a prominent cause for liver cirrhosis. MASH-cirrhosis is responsible for liver complications and there is no specific treatment. To develop new therapeutic approaches, animal models are needed. The aim of this study was to develop a fast animal model of MASH-cirrhosis in rats reflecting the human disease. Carbon tetrachloride (CCl4) injections in combination with a high-fat Western diet (WD) were used to induce MASH-cirrhosis. To accelerate liver injury, animals received phenobarbital (PB) in their drinking water using two different regimens. Rats developed advanced MASH-cirrhosis characterized by portal hypertension, blood biochemistry, hepatic ballooning, steatosis, inflammation and fibrosis. Importantly, rats receiving low-dose PB for the long term (LT) showed ascites after 6 weeks, whereas rats with high-dose short-term (ST) PB developed ascites after 8 weeks. ST- and LT-treated rats showed increased portal pressure (PP) and decreased mean arterial pressure (MAP). Of note, hepatocyte ballooning was only observed in the LT group. The LT administration of low-dose PB with CCl4 intoxication and WD represents a fast and reproducible rat model mimicking decompensated MASH-cirrhosis in humans. Thus, CCl4 + WD with LT low-dose phenobarbital treatment might be the preferred rat animal model for drug development in MASH-cirrhosis.
Activation of Mammary Epithelial and Stromal Fibroblasts upon Exposure to Escherichia coli Metabolites
Authors: Alshehri Jamilah H. , Al-Nasrallah Huda K. , Al-Ansari Mysoon M. , Aboussekhra Abdelilah
Published: October 2024

Breast cancer is the leading cause of cancer death among women worldwide. The mammary gland is composed of various types of cells including luminal cells, fibroblasts, immune cells, adipocytes, and specific microbiota. The reciprocal interaction between these multiple types of cells can dictate the initiation and progression of cancer, as well as metastasis and response to therapy. In the present report, we have shown that Escherichia coli-conditioned media (E-CM) can directly activate human mammary luminal epithelial cells (HMLEs), by inducing epithelial-to-mesenchymal transition (EMT), a process associated with increased proliferation and invasion capacities, as well as stemness features. Additionally, it has been shown that E-CM has an indirect pro-carcinogenic effect, mediated by the activation of normal breast fibroblasts (NBFs). Indeed, E-CM upregulated various markers of active fibroblasts (FAP-α, GPR77, and CD10), and enhanced the proliferation, migration, and invasion capacities of NBFs. Furthermore, E-CM induced an inflammatory response in NBFs by activating the pro-inflammatory NF-kB transcription factor and several of its downstream target cytokines including IL-1β, IL-6, and IL-8. This E-CM-dependent activation of NBFs was confirmed by showing their paracrine pro-carcinogenic effects through inducing EMT and stemness features in normal breast epithelial cells. Interestingly, similar effects were obtained by recombinant human IL-1β. These results provide the first indication that E. coli can initiate breast carcinogenesis through the activation of breast stromal fibroblasts and their paracrine pro-carcinogenic effects.
Complement C5a Implication in Axonal Growth After Injury
Authors: Cotten Aurélie , Jeanneau Charlotte , Decherchi Patrick , About Imad
Published: October 2024

Complement C5a protein has been shown to play a major role in tissue regeneration through interaction with its receptor (C5aR) on target cells. Expression of this receptor has been reported in the nervous system which, upon injury, has no treatment to restore the lost functions. This work aimed at investigating the Complement C5a effect on axonal growth after axotomy in vitro. Primary hippocampal neurons were isolated from embryonic Wistar rats. Cell expression of C5aR mRNA was verified by RT-PCR while its membrane expression, localization, and phosphorylation were investigated by immunofluorescence. Then, the effects of C5a on injured axonal growth were investigated using a 3D-printed microfluidic device. Immunofluorescence demonstrated that the primary cultures contained only mature neurons (93%) and astrocytes (7%), but no oligodendrocytes or immature neurons. Immunofluorescence revealed a co-localization of NF-L and C5aR only in the mature neurons where C5a induced the phosphorylation of its receptor. C5a application on injured axons in the microfluidic devices significantly increased both the axonal growth speed and length. Our findings highlight a new role of C5a in regeneration demonstrating an enhancement of axonal growth after axotomy. This may provide a future therapeutic tool in the treatment of central nervous system injury.
A Novel Mechanism Based on Oxygen Vacancies to Describe Isobutylene and Ammonia Sensing of p-Type Cr2O3 and Ti-Doped Cr2O3 Thin Films
Authors: Zhou Pengfei , Tsang Jone-Him , Blackman Chris , Shen Yanbai , Liang Jinsheng , Covington James A. , Saffell John , Danesh Ehsan
Published: October 2024

Gas sensors based on metal oxide semiconductors (MOS) have been widely used for the detection and monitoring of flammable and toxic gases. In this paper, p-type Cr2O3 and Ti-doped Cr2O3 (CTO) thin films were synthesized using an aerosol-assisted chemical vapor deposition (AACVD) method. Detailed analysis of the thin films deposited, including structural information, their elemental composition, oxidation state, and morphology, was investigated using XRD, Raman analysis, SEM, and XPS. All the gas sensors based on pristine Cr2O3 and CTO exhibited a reversible response and good sensitivity to isobutylene (C4H8) and ammonia (NH3) gases. Doping Ti into the Cr2O3 lattice improves the response of the CTO-based sensors to C4H8 and NH3. We describe a novel mechanism for the gas sensitivity of p-type metal oxides based on variations in the oxygen vacancy concentration.
Fast Determination and Source Apportionment of Eight Polycyclic Aromatic Hydrocarbons in PM10 Using the Chemometric-Assisted HPLC-DAD Method
Authors: Hu Ting , Xia Yitao , Wang You , Lin Li , An Rong , Xu Ling , Qing Xiangdong
Published: October 2024

Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds that are both toxic and hazardous to human health and ecological systems. In recent work, a novel analytical strategy based on the chemometric-assisted HPLC-DAD method was proposed for the quantification and source apportionment of eight PAHs in PM10 samples. Compared to traditional chromatographic methods, this approach does not require the purification of complex PM10 samples. Instead, it utilizes a mathematical separation method to extract analytes’ profiles from overlapping chromatographic peaks, enabling precise quantification of PAHs in PM10. Firstly, 40 PM10 samples collected in Loudi city during two sampling periods were used for analysis. Subsequently, the second-order calibration method based on alternating trilinear decomposition (ATLD) was employed to handle the three-way HPLC-DAD data. Finally, the pollution sources of PAHs were analyzed by the feature component analysis method according to the obtained relative concentration matrix. For the validation model, the average recoveries of eight PAHs were between (88.8 ± 7.6)% and (105.6 ± 7.5)%, and the root-mean-square errors of prediction ranged from 0.03 μg mL−1 to 0.47 μg mL−1. The obtained limits of quantification for eight PAHs were in the range of 0.0050 μg mL−1 to 0.079 μg mL−1. For actual PM10 samples, results of the feature component analysis indicated that the main source of PAHs in PM10 may be traffic emissions and coal combustion. In summary, the proposed method provided a new and rapid analysis method for the accurate determination and source apportionment of PAHs in atmospheric aerosols.
Selective Extrauterine Placental Perfusion in Monochorionic Twins Is Feasible—A Case Series
Authors: Kuehne Benjamin , Trieschmann Jan , Butzer Sarina Kim , Mehler Katrin , Gottschalk Ingo , Kribs Angela , Oberthuer André
Published: October 2024

Background: Monochorionic (MC) twins are at risk for severe twin-to-twin transfusion syndrome (TTTS) or twin anemia-polycythemia sequence (TAPS). In the case of preterm delivery, cesarean section (CS) with immediate umbilical cord clamping (ICC) of both twins is usually performed. While the recipient is at risk for polycythemia and may benefit from ICC, this procedure may result in aggravation of anemia with increased morbidity in the anemic donor. The purpose of this study was to demonstrate that the novel approach of selective extrauterine placental perfusion (EPP) with delayed umbilical cord clamping (DCC) in the donor infant is feasible in neonatal resuscitation of MC twins and may prevent severe anemia in donor and polycythemia in the recipient. Methods: Preterm MC twins with antenatal suspected severe anemia of the donor as measured by Doppler ultrasound, born with birthweights < 1500 g by CS, were transferred to the neonatal resuscitation unit with placenta and intact umbilical cords. In the donor, the umbilical cord was left intact to provide DCC with parallel respiratory support (EPP approach), while the cord of the recipient was clamped immediately after identification. Results: Selective EPP was performed in three cases of MC twins with TAPS and acute peripartum TTTS. All donor twins had initial hemoglobin levels ≥ 13.0 g/dL, and none of them required red blood cell transfusion on the first day after birth. Conclusions: Selective EPP may be a feasible strategy for neonatal resuscitation of MC preterm twins with high stage TAPS and TTTS to prevent anemia-related morbidities and may improve infant outcome.
Association of SLC19A1 Gene Polymorphisms and Its Regulatory miRNAs with Methotrexate Toxicity in Children with Acute Lymphoblastic Leukemia
Authors: Karpa Vasiliki , Kalinderi Kallirhoe , Gavriilaki Eleni , Antari Vasiliki , Hatzipantelis Emmanuil , Katopodi Theodora , Fidani Liana , Tragiannidis Athanasios
Published: October 2024

Methotrexate (MTX) is an anti-folate chemotherapeutic agent that is considered to be a gold standard in Acute Lymphoblastic Leukemia (ALL) therapy. Nevertheless, toxicities induced mainly due to high doses of MTX are still a challenge for clinical practice. MTX pharmacogenetics implicate various genes as predictors of MTX toxicity, especially those that participate in MTX intake like solute carrier family 19 member 1 (SLC19A1). The aim of the present study was to evaluate the association between SLC19A1 polymorphisms and its regulatory miRNAs with MTX toxicity in children with ALL. A total of 86 children with ALL were included in this study and were all genotyped for rs2838958, rs1051266 and rs1131596 SLC19A1 polymorphisms as well as the rs56292801 polymorphism of miR-5189. Patients were followed up (48, 72 and 96 h) after treatment with MTX in order to evaluate the presence of MTX-associated adverse events. Our results indicate that there is a statistically significant correlation between the rs1131596 SLC19A1 polymorphism and the development of MTX-induced hepatotoxicity (p = 0.03), but there is no significant association between any of the studied polymorphisms and mucositis or other side effects, such as nausea, emesis, diarrhea, neutropenia, skin rash and infections. In addition, when genotype TT of rs1131596 and genotype AA of rs56292801 are both present in a patient then there is a higher risk of developing severe hepatotoxicity (p = 0.0104).
Reducing Energy Consumption Using DOE and SPC on Cork Agglomeration Line
Authors: Silva Hugo , Santos André S. , Varela Leonilde R.
Published: October 2024

The industrial landscape has revealed two trends: increased competitiveness and a greater demand for sustainable solutions. Materials with cork in their composition are an appealing solution, since they guarantee the desired mechanical characteristics while contributing to the prevention of environmental degradation. Given the change in external factors, there has been a substantial rise in energy costs. Thus, it is essential to optimize processes, with the aim of reducing the consumption of resources, such as electricity. This project was developed at a company that manufactures cork blocks, sheets, and rolls. Regarding blocks, a critical operation of this line is the high-frequency heating, being the bottleneck of this work center. With the critical variables previously identified, planned experiments were conducted based on DOE’s full factorial methodology. Two out of four products revealed inputs with statistical significance. With these results, a reduction in parameters was implemented in the factors and interactions that showed no statistical significance. Finally, average and amplitude control charts, based on the SPC methodology, were applied to solidify and guarantee the quality of the agglomerated blocks, with the parameter changes already introduced. The company benefited from this study by having a significant reduction in its energy consumption.
Physical-Vapor-Deposition-Coated Natural Rocks as Sustainable Cutting Material: First Insights into the Effect of Substrate Integrity on Properties of TiN Thin Film
Authors: Petersen Hilke , Graf Dominic , Lopes Dias Nelson Filipe , Tillmann Wolfgang , Wolters Philipp Dan Hendrik , Bergmann Benjamin , Breidenstein Bernd
Published: October 2024

The most important cutting materials for machining are carbides. Their production requires both tungsten and cobalt; however, these materials are becoming increasingly difficult to obtain and are sometimes mined under ethically questionable conditions. As a result, increasing efforts are being made to expand the range of cutting materials. The basic suitability of natural rocks for cutting tools in less demanding processes has already been demonstrated. PVD coating of the natural rocks could improve their performance. The adhesion mechanisms in TiN-coated natural rock samples are discussed below. The TiN thin film is characterized in depth.
Genetic Variation in the Invaded Population of the Fall Armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), in India
Authors: Kavyashree Bediganahally Annegowda , Deshmukh Sharanabasappa Shrimantara , Satish Kundur Mahadevappa , Kalleshwaraswamy Chicknayakanahalli Marulsiddappa , Sridhara Shankrappa , Satish Danappagala , Acharya Rajendra
Published: October 2024

The fall armyworm, Spodoptera frugiperda, an invasive, polyphagous pest, causes significant damage to corn. The majority of insects rely on a broad range of digestive enzymes and an intricate detoxifying mechanism to consume chemically diverse host plants. The genetic variation in S. frugiperda was analyzed using cytochrome oxidase subunit I (COI) and triose phosphate isomerase (Tpi). In addition, a new attempt was made to determine the variation with respect to two detoxifying genes, viz., carboxylesterase and glutathione S-transferase. The highest genetic variation (3.03%) was found between the S. frugiperda populations of Tirupati and Delhi with respect to the Tpi gene and between the populations of Punjab and Hosur regarding COI (3.30%). The results for various genes revealed that populations of the fall armyworm were homogeneous, showing low genetic distance using COI, ranging from 0.40 to 3.30%, and, using Tpi, ranging from 0.43 to 3.03%. The variation in carboxylesterase and glutathione S-transferase ranged from 0.04 to 0.15% and from 0.01 to 0.02, respectively. Amino acid sequences were also produced using DNA sequences from several fall armyworm populations. Populations in Tirupati, Solapur, and Hyderabad shared 98.7% of their sequence with that in Delhi. Fall armyworm amino acid sequences showed 79.7 to 82.0% identity with S. exigua and 69.6 to 73.0% identity with S. litura. Our study provides vital information for understanding the genetic variation in the fall armyworm following its invasion of India.
High-Temperature SHS Heat Insulators Based on Pre-Activated Mineral Raw Materials
Authors: Sadykov Bakhtiyar , Khairullina Ainur , Artykbayeva Aida , Maten Alua , Zhapekova Anar , Osserov Timur , Bakkara Ayagoz
Published: October 2024

In this paper, the results of the technological combustion of SHS heat insulators based on mineral origins are presented. It is shown that after mechanochemical treatment of minerals—diatomite—the kinetic characteristics of the combustion process change, providing targeted formation of the phase composition, structure, and properties of the SHS composite. A positive effect of using various modifiers during the MCT of diatomite—the activation of the combustion process—was established. The selection of modifiers provides an increase in the strength of the synthesized SHS composites as a result of the formation of aluminate compounds in the synthesis products, and a decrease in thermal conductivity to 0.157 W/m*K due to the formation of the ultraporous structure of the samples.
Correction: Slišković et al. A Systematic Analysis of the Mediterranean Sea (IHO Sea Area) in the WRiMS Database. Diversity 2024, 16, 358
Authors: Slišković Merica , Gudelj Anita , Piria Marina
Published: October 2024

In the published article [...]
Inflammatory Prostatitis Plus IBS-D Subtype and Correlation with Immunomodulating Agent Imbalance in Seminal Plasma: Novel Combined Treatment
Authors: Castiglione Roberto , Bertino Gaetano , Vicari Beatrice Ornella , Rizzotto Agostino , Sidoti Giuseppe , D’Agati Placido , Salemi Michele , Malaguarnera Giulia , Vicari Enzo
Published: October 2024

We recently demonstrated the effectiveness of long-term treatment with rifaximin and the probiotic DSF (De Simone formulation) in improving urogenital and gastrointestinal symptoms in patients with both chronic inflammatory prostatitis (IIIa prostatitis) and diarrhea-predominant irritable bowel syndrome (IBS-D), relative to patients with IBS-D alone. Because the low-grade inflammation of the intestine and prostate may be one of the reasons for co-developing both IIIa prostatitis and IBS-D, we designed the present study to once again evaluate the efficacy of combined rifaximin and DSF treatment in patients affected by IIIa prostatitis plus IBS-D, but we also measured seminal plasma pro-inflammatory (IL-6) and anti-inflammatory (IL-10) cytokines before and after treatment. Methods: We consecutively enrolled 124 patients with IIIa prostatitis and IBS-D (diagnosed using the Rome III criteria). Patients were randomized into two groups: group A (n = 64) was treated with rifaximin (seven days per month for three months) followed by DSF, and group B (n = 60) was treated with a placebo. By the end of the intervention, 68.7% and 62.5% of patients from group A reported improved NIH-CPSI (National Institute of Health’s Chronic Prostatitis Symptom Index) and IBS-SSS (Irritable Bowel Syndrome Severity Scoring System) scores, respectively, compared to only 3.3% and 5% of the placebo group. Group A patients also had significantly lower mean seminal plasma levels of IL-6 (11.3 vs. 32.4 pg/mL) and significantly higher mean levels of IL-10 (7.9 vs. 4.4 pg/mL) relative to baseline, whereas the levels of IL-6 and IL-10 did not change in the placebo group. Conclusions: The combined treatment with rifaximin and DSF appears to represent the optimal approach for addressing a syndrome such as irritable bowel syndrome (IBS-D plus), which frequently co-occurs with prostatitis (IIIa prostatitis). This approach is particularly beneficial in cases where the symptoms are not always clearly delineated, the etiology is multifactorial, and the diagnosis is multilevel.
A Geometric Significance-Aware Deep Mutual Learning Network for Building Extraction from Aerial Images
Authors: Hao Ming , Lin Huijing , Chen Shilin , Luo Weiqiang , Zhang Hua , Zheng Nanshan
Published: October 2024

Knowledge-driven building extraction method exhibits a restricted adaptability scope and is vulnerable to external factors that affect its extraction accuracy. On the other hand, data-driven building extraction method lacks interpretability, heavily relies on extensive training data, and may result in extraction outcomes with building boundary blur issues. The integration of pre-existing knowledge with data-driven learning is essential for the intelligent identification and extraction of buildings from high-resolution aerial images. To overcome the limitations of current deep learning building extraction networks in effectively leveraging prior knowledge of aerial images, a geometric significance-aware deep mutual learning network (GSDMLNet) is proposed. Firstly, the GeoSay algorithm is utilized to derive building geometric significance feature maps as prior knowledge and integrate them into the deep learning network to enhance the targeted extraction of building features. Secondly, a bi-directional guidance attention module (BGAM) is developed to facilitate deep mutual learning between the building feature map and the building geometric significance feature map within the dual-branch network. Furthermore, the deployment of an enhanced flow alignment module (FAM++) is utilized to produce high-resolution, robust semantic feature maps with strong interpretability. Ultimately, a multi-objective loss function is crafted to refine the network’s performance. Experimental results demonstrate that the GSDMLNet excels in building extraction tasks within densely populated and diverse urban areas, reducing misidentification of shadow-obscured regions and color-similar terrains lacking building structural features. This approach effectively ensures the precise acquisition of urban building information in aerial images.
Econometric Analysis of the Sustainability and Development of an Alternative Strategy to Gross Value Added in Kazakhstan’s Agricultural Sector
Authors: Tleubayev Azat , Kerimkhulle Seyit , Tleuzhanova Manatzhan , Uchkampirova Aigul , Bulakbay Zhanat , Mugauina Raikhan , Tazhibayeva Zhumagul , Adalbek Alibek , Iskakov Yerassyl , Toleubay Daniyar
Published: October 2024

Based on the systematization of relevant problems in the agricultural sector of Kazakhstan and other countries, the purpose of the research is to aid in the development and implementation of a methodology for the econometric analysis of sustainability, the classification of economic growth, and an alternative strategy for gross value added depending on time phases with time lags of 0, 1, and 2 years, and on the gross fixed capital formation in the agricultural sector of Kazakhstan. The research has used a variety of quantitative techniques, including the logistic growth difference equation, applied statistics, econometric models, operations research, nonlinear mathematical programming models, economic modeling simulations, and sustainability analysis. In the work on three criteria: equilibrium, balanced and optimal growth, we have defined the main trends of growth of Gross added value of agriculture, hunting and forestry. The first, depending on the time phases, the second, depending on the Gross fixed capital formation transactions for equilibrium growth, for the growth of an alternative strategy, for the endogenous growth rate and the growth of exogenous flows. And we also received a classification of the trend of Productive, Moderate and Critical growth for the agricultural industry depending on the correlated linkaged industry of the national economy of Kazakhstan. The results of this work can be used in data analytics and artificial intelligence, digital transformation and technology in agriculture, as well as in the areas of sustainability and environmental impact.
Private Educational Expenditure Inequality between Migrant and Urban Households in China’s Cities
Authors: Chen Yiwen , Salagean Ioana , Zou Benteng
Published: October 2024

This paper studies households’ expenditure on the education of children in China’s cities to assess how internal migrant families’ investment in the human capital of their offspring differs from that of local urban families. The private education-related expenditure reflects both households’ willingness to invest in human capital and institutional constraints, as China’s household registration (hukou) system prevents children without a local city hukou from enrolling in urban public schools. In-school fees (consisting of statutory tuition and institutional tuition fees) are commonly topped-up with substantial private tutoring expenditure. We apply multiple regression to the 2008 “Rural–Urban Migration Survey in China” data to analyze the average expenditure differences between temporary migrants, permanent migrants and locals. The findings are, after controlling for social and economic characteristics, that: (1) the overall spending on education of migrant households overwhelmingly exceeds that of locals, which is expected since migrants must finance privately all education-related expenses their children incur; (2) migrant households spend more on institutional tuition fees compared to households with a local city hukou, reflecting the varying severity of administrative hurdles faced by families; and (3) temporary migrants spend more on institutional tuition fees than permanent migrant households, but less in private tutoring.
A High-Performance FPGA-Based RoCE v2 RDMA Packet Parser and Generator
Authors: Sun Zezheng , Guo Zhichuan , Ma Jiandong , Pan Yipeng
Published: October 2024

RDMA (Remote Direct Memory Access) technology has been widely applied due to its high-throughput and low-latency characteristics compared with traditional networks. Implementing RDMA with an FPGA (Field-Programmable Gate Array) is a feasible solution. This paper proposes an implementation method for the ROCE v2 (Remote Direct Memory Access) protocol packet parser and generator based on an FPGA, capable of supporting various transaction packet types, such as RDMA READ, RDMA WRITE, and SEND, under the Reliable Connection service. The RDMA READ and RDMA WRITE performance of RDMA is close to 100 Gbps, which provides a feasible solution for the application of wide-area networks.
A Power Control and Intervention Algorithm for Co-Existing IMT Base Stations and Satellite Services
Authors: Jia Min , Meng Shiyao , Wang Hui , Tang Zhouhao , Jin Ziliang
Published: October 2024

IMT-2020 (International Mobile Telecommunications-2020) is the prevailing mobile communication technology at the moment, significantly affecting societal progress. Nevertheless, the roll-out of the IMT-2020 system has introduced numerous interferences to existing services. The coexistence with fixed satellite services has become a topical issue currently under consideration. This paper discusses the compatibility and interference issues between IMT-2020 and the 14 GHz FSS (fixed-satellite service) uplink, as well as the spectrum access issue solved by artificial intelligence methods. The study shows that the interference from IMT-2020 macro-base stations to FSS space stations exceeds the ITU standard by approximately 10 dB. To control the interference, a partition-based power control algorithm is proposed, which divides ground base stations into multiple areas and virtualizes each area’s base stations into a single large base station then applies power control to maximize the total transmission power of the base stations within the area. Furthermore, three intra-partition power control algorithms are introduced: average power allocation, power allocation based on channel gain, andna power allocation method based on the maximum intra-partition sum rate. Additionally, under the assumption that dynamic satellite nodes are available in the system for ground user access, a spectrum access algorithm utilizing deep reinforcement learning is designed. Simulation results confirm the effectiveness of the proposed scheme, which can reduce the interference from the IMT-2020 system to the FSS service below the threshold, ensuring harmonious coexistence of the two services.
Comparison of Large Language Models in Generating Machine Learning Curricula in High Schools
Authors: Noveski Gjorgji , Jeroncic Mathis , Velard Thomas , Kocuvan Primož , Gams Matjaž
Published: October 2024

With the rapid advancement of artificial intelligence technologies, the integration of AI concepts into educational curricula represents an increasingly important issue. This paper presents a comparative analysis of four AI large language models, ChatGPT (now GPT-4o), Bard (now Gemini), Copilot, and Auto-GPT, in the last year, progressing from the previous into the newer versions, thus also revealing the progress over time. Tasks were selected from the Valence project, which aims to advance machine learning in high school education with material designed by human experts. The four LLMs were assessed across 13 topics, 35 units, and 12 code segments, focusing on their code generation, definition formulation, and textual task capabilities. The results were analyzed using various metrics to conduct a comprehensive evaluation. Each LLM was allowed up to five attempts to produce outputs closely aligned with human-written materials, with experts providing iterative feedback. This study evaluated the effectiveness and accuracy of these LLMs in educational content creation, offering insights into their potential roles in shaping current and future AI-centric education systems.