bluuebunny/crossref_metadata_2025 huggingface dataset:
Modified Lignin-Based Cement Solidifying Material for Improving Engineering Residual Soil
Authors: Yu Xiang , Lu Hongbo , Peng Jie , Ren Jinming , Wang Yongmin , Chen Junhao
Published: November 2023

Although lignin improves the strength and modulus of soil, it is less active when unmodified, and it exhibits more limited effects on soils in combination with traditional Ca-based curing agents. Lignin-solidified soil also exhibits deficiencies, such as poor durability under dry–wet cycling conditions, and thus, the amelioration effect is limited. This study investigated the enhancement of cement-solidified soil using hydroxylated lignin with sodium silicate and quicklime used as activators to improve the engineering performance and durability of the treated soil. Using respective cement, sodium silicate, quicklime, and lignin contents of 7%, 0.4%, 0.2%, and 0.2% with respect to the dry mass of the slag soil, the strength and cohesion of the composite-solidified soil were 1.5 times those of cement-solidified soil, whereas the internal friction angle increased by 5.1°. At a solidifying age of 14 d, the penetration resistance almost doubled, indicating a significant improvement in the bearing capacity of the soil. The results suggest that modified lignin-based admixtures may significantly enhance the performance of cement-solidified soil. The cement curing admixture used in this study provides theoretical and technological support for curing agent preparation and the utilization of slag.
Design and Optimization of Heat Treatment Process Parameters for High-Molybdenum-Vanadium High-Speed Steel for Rolls
Authors: Chen Jibing , Liu Yanfeng , Wang Yujie , Xu Rong , Shi Qianyu , Chen Junsheng , Wu Yiping
Published: November 2023

High-molybdenum-vanadium high-speed steel is a new type of high-hardenability tool steel with excellent wear resistance, castability, and high-temperature red hardness. This paper proposes a composition design of high-molybdenum-vanadium high-speed steel for rolls, and its specific chemical composition is as follows (wt.%): C2%, Mo7.0%, V7.0%, Si0.3%, Mn0.3%, Ni0.4%, Cr3.0%, and the rest of the iron. This design is characterized by the increase in molybdenum and vanadium in high-speed steel to replace traditional high-speed steel rolls with the tungsten element in order to reduce the heavy elements’ tungsten-specific gravity segregation caused by centrifugal casting so that the roll performance is uniform and the stability of use is improved. JMatPro (version 7.0) simulation software is used for the composition design of high-molybdenum-vanadium high-speed steel. The phase composition diagram is analyzed under different temperatures. The content of different phases of the organization in different temperatures is also studied. The martensitic transformation temperature and different tempering temperatures with the different types of compounds and grain sizes are calculated. The process parameters of heat treatment of high-molybdenum-vanadium high-speed steel are optimized. The selection of carbon content and the temperature of M50 are calculated and optimized, and the results show that the range of pouring temperatures, quenching temperatures, annealing temperatures, and tempering temperatures are 1360~1410 °C, 1190~1200 °C, 818~838 °C, and 550~600 °C, respectively. Scanning electron microscope (SEM) analysis of the samples obtained by using the above heat treatment parameters is consistent with the simulation results, which indicates that the simulation has important reference significance for guiding the actual production.
A Nanocomposite Sol-Gel Film Based on PbS Quantum Dots Embedded into an Amorphous Host Inorganic Matrix
Authors: Elisa Mihail , Sava Bogdan Alexandru , Eftimie Mihai , Nicoara Adrian Ionut , Vasiliu Ileana Cristina , Rusu Madalin Ion , Bartha Cristina , Enculescu Monica , Kuncser Andrei Cristian , Oane Mihai , Aguado César Elosúa , López-Torres Diego
Published: November 2023

In this study, a sol-gel film based on lead sulfide (PbS) quantum dots incorporated into a host network was synthesized as a special nanostructured composite material with potential applications in temperature sensor systems. This work dealt with the optical, structural, and morphological properties of a representative PbS quantum dot (QD)-containing thin film belonging to the Al2O3–SiO2–P2O5 system. The film was prepared using the sol-gel method combined with the spin coating technique, starting from a precursor solution containing a suspension of PbS QDs in toluene with a narrow size distribution and coated on a glass substrate in a multilayer process, followed by annealing of each deposited layer. The size (approximately 10 nm) of the lead sulfide nanocrystallites was validated by XRD and by the quantum confinement effect based on the band gap value and by TEM results. The photoluminescence peak of 1505 nm was very close to that of the precursor PbS QD solution, which demonstrated that the synthesis route of the film preserved the optical emission characteristic of the PbS QDs. The photoluminescence of the lead sulfide QD-containing film in the near infrared domain demonstrates that this material is a promising candidate for future sensing applications in temperature monitoring.
Fractional Hermite–Hadamard-Type Inequalities for Differentiable Preinvex Mappings and Applications to Modified Bessel and q-Digamma Functions
Authors: Tariq Muhammad , Ahmad Hijaz , Shaikh Asif Ali , Ntouyas Sotiris K. , Hınçal Evren , Qureshi Sania
Published: November 2023

The theory of convexity pertaining to fractional calculus is a well-established concept that has attracted significant attention in mathematics and various scientific disciplines for over a century. In the realm of applied mathematics, convexity, particularly in relation to fractional analysis, finds extensive and remarkable applications. In this manuscript, we establish new fractional identities. Employing these identities, some extensions of the fractional H-H type inequality via generalized preinvexities are explored. Finally, we discuss some applications to the q-digamma and Bessel functions via the established results. We believe that the methodologies and approaches presented in this work will intrigue and spark the researcher’s interest even more.
The Consecutive 200 Cases of Endoscopic-Combined Intrarenal Surgery: Comparison between Standard and Miniature Surgeries
Authors: Moon Young Joon , Cho Kang Su , Jung Dae Chul , Chung Doo Yong , Lee Joo Yong
Published: November 2023

Background and Objectives: Percutaneous nephrolithotomy (PCNL) is still the gold-standard treatment for large and/or complex renal stones. Endoscopic combined intrarenal surgery (ECIRS) was developed with the goal of minimizing the number of access tracts of PCNL while simultaneously improving the one-step stone-free rate (SFR). The aim of this study was to share the experience of the consecutive 200 cases of ECIRS in one institute and analyze surgical outcomes of mini-ECIRS and standard ECIRS. Materials and Methods: We performed ECIRS for 200 adult patients between July 2017 and January 2020. An ECIRS was performed with the patient under general anesthesia in the intermediate-supine position. Surgeries were finished using a tubeless technique with a simple ureteral stent insertion. Results: There were significant differences in the mean maximal stone length (MSL), the variation coefficient of stone density (VCSD), the linear calculus density (LCD), the Seoul National University Renal Stone Complexity (S-ReSC), and the modified S-ReSC scores in stone characteristics, and estimated blood loss (EBL) and operation time in peri-operative outcomes between conventional and mini-ECIRS. After propensity-score matching, there was only a difference in EBL between the two groups. In logistic regression models, MSL [odds ratio (OR) 0.953; 95% confidence interval (CI) 0.926–0.979; p < 0.001], LCD (OR 4.702; 95% CI 1.613–18.655; p = 0.013) were significant factors for the success rate after ECIRS. Conclusions: In patients who underwent a mini-ECIRS, the stones were relatively smaller and less complex, and the operation time was shorter. However, if the size of stones was similar, there was no difference in the success rate, but EBL was lower in mini-ECIRS than in standard surgery.
Investigation on the Metal Transfer and Cavity Evolution during Submerged Arc Welding with X-ray Imaging Technology
Authors: Han Yanfei , Jia Chuanbao , He Chen , Zhang Maofu , Maksymov Sergii , Wu Chuansong
Published: November 2023

The physical phenomena of submerged arc welding (SAW) conducted with a 1.6 mm flux-cored wire were investigated using X-ray imaging technique. Three kinds of metal transfer modes were confirmed in this paper, namely the front flux wall-guided droplet transfer, back flux wall-guided droplet transfer, and repelled droplet transfer, of which the corresponding percentages were 47.65%, 45.29%, and 7.06%, respectively. Although the average sizes of the droplets for SAW and FCAW (flux-cored wire welding) were 2.0 mm and 1.9 mm with an average droplet transfer time of 90.3 ms, it required 36.4% more time for the droplet of SAW to finish one metal transfer than it did in FCAW. In addition, the volume of the cavity was not constant but repeated a cycle mode of “expansion and contraction” during the whole process. Thus, the dynamics of the cavity and viscous resistance caused by the flux collectively slowed down the velocity of the droplets from the wire to the weld pool in SAW. Compared with FCAW, a smoother weld without pits and pores was manufactured during the SAW process. Due to the compression effect of the flux, the 14.5 mm average weld width of SAW was 2.9 mm shorter than that of the FCAW. Furthermore, the thickness of slag with a porous structure in SAW was 2.7 times of that in FCAW, indicating that it could provide better protection to the weld of SAW.
Numerical Study of the Time–Periodic Electroosmotic Flow of Viscoelastic Fluid through a Short Constriction Microchannel
Authors: Ji Jianyu , Qian Shizhi , Parker Armani Marie , Zhang Xiaoyu
Published: November 2023

Electroosmotic flow (EOF) is of utmost significance due to its numerous practical uses in controlling flow at micro/nanoscales. In the present study, the time–periodic EOF of a viscoelastic fluid is statistically analyzed using a short 10:1 constriction microfluidic channel joining two reservoirs on either side. The flow is modeled using the Oldroyd-B (OB) model and the Poisson–Boltzmann model. The EOF of a highly concentrated polyacrylamide (PAA) aqueous solution is investigated under the combined effects of an alternating current (AC) electric field and a direct current (DC) electric field. Power-law degradation is visible in the energy spectra of the velocity fluctuations over a wide frequency range, pointing to the presence of elastic instabilities in the EOF. The energy-spectra curves of the velocity fluctuations under a DC electric field exhibit peaks primarily beneath 20 Hz, with the greatest peak being observed close to 6 Hz. When under both DC and AC electric fields, the energy spectra of the velocity fluctuations exhibit a peak at the same frequency as the AC electric field, and the highest peak is obtained when the frequency of the AC electric field is near 6 Hz. Additionally, the frequency of the AC electric field affects how quickly the viscoelastic EOF flows. Higher flow rates are obtained at relatively low frequencies compared to under the DC electric field, and the greatest flow rate is found close to 6 Hz. But as the frequency rises further, the flow rate falls. The flow rate falls to a level below the DC electric field when the frequency is sufficiently high.
Physics-Based Signal Analysis of Genome Sequences: An Overview of GenomeBits
Authors: Canessa Enrique
Published: November 2023

A comprehensive overview of the recent physics-inspired genome analysis tool, GenomeBits, is presented. This is based on traditional signal processing methods such as discrete Fourier transform (DFT). GenomeBits can be used to extract underlying genomics features from the distribution of nucleotides, and can be further used to analyze the mutation patterns in viral genomes. Examples of the main GenomeBits findings outlining the intrinsic signal organization of genomics sequences for different SARS-CoV-2 variants along the pandemic years 2020–2022 and Monkeypox cases in 2021 are presented to show the usefulness of GenomeBits. GenomeBits results for DFT of SARS-CoV-2 genomes in different geographical regions are discussed, together with the GenomeBits analysis of complete genome sequences for the first coronavirus variants reported: Alpha, Beta, Gamma, Epsilon and Eta. Interesting features of the Delta and Omicron variants in the form of a unique ‘order–disorder’ transition are uncovered from these samples, as well as from their cumulative distribution function and scatter plots. This class of transitions might reveal the cumulative outcome of mutations on the spike protein. A salient feature of GenomeBits is the mapping of the nucleotide bases (A,T,C,G) into an alternating spin-like numerical sequence via a series having binary (0,1) indicators for each A,T,C,G. This leads to the derivation of a set of statistical distribution curves. Furthermore, the quantum-based extension of the GenomeBits model to an analogous probability measure is shown to identify properties of genome sequences as wavefunctions via a superposition of states. An association of the integral of the GenomeBits coding and a binding-like energy can, in principle, also be established. The relevance of these different results in bioinformatics is analyzed.
Correction: Matthew et al. A Loop-Mediated Isothermal Amplification (LAMP) Assay Specific to Trichomonas tenax Is Suitable for Use at Point-of-Care. Microorganisms 2022, 10, 594
Authors: Matthew Maurice A. , Christie Jevan , Yang Nawu , Yao Chaoqun
Published: November 2023

In the original publication [...]
First Report on Leptospira Species Isolated from Patients in Slovenia
Authors: Ružić-Sabljić Eva , Podgoršek Daša , Strašek Smrdel Katja , Celar Šturm Andraž , Logar Mateja , Pavlović Andrea , Remec Tatjana , Baklan Zvonko , Pal Emil , Cerar Kišek Tjaša
Published: November 2023

Leptospirosis is an important worldwide zoonosis, and it has also been reported in Slovenia. The cultivation of Leptospira from human material is difficult. Despite that, we successfully isolated 12 human Leptospira strains isolated from patients between 2002 and 2020 and used various methods for the phenotypic and genotypic characterization of the strains, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) using our own MALDI-TOF data library, melting temperature analysis of the amplified lfb1 gene, determination of Leptospira serogroups using rabbit immune sera, NotI-RFLP of the whole Leptospira genome, multilocus sequence typing (MLST) of seven housekeeping genes, and whole-genome sequencing (WGS)-based typing. We confirmed the presence of four pathogenic Leptospira species (L. kirschneri, L. interrogans, L. borgpetersenii, and L. santarosai) and three serogroups: Grippotyphosa, Icterohaemorrhagiae, and Sejroe. MALDI-TOF identified three of seven isolates at the species level and four isolates at the genus level. Serovars of 8 of the 10 strains were determined using NotI-RFLP. MLST showed that the clinical isolates belonged to sequence types ST17, ST110, and ST155. WGS confirmed the analysis of Leptospira strains using conventional methods. In addition, WGS provided better taxonomic resolution for isolate DDA 10944/10.
Karstification and Its Role in the Formation of Carbonate Reservoirs: A Case Study from the Ordovician Majiagou Formation in Jingbian, Ordos Basin, North China
Authors: Peng Xiaoxia , Li Guobin , Cheng Xin , Guo Ling
Published: November 2023

The discovery of natural gas reserves in Lower Paleozoic carbonate formations has generated significant enthusiasm regarding the potential for oil and gas exploration in the deeper carbonate reservoirs of the Ordos Basin. Significant progress has been made in the field of natural gas exploration, especially within the carbonate reservoirs of the Ordovician Majiagou Formation. In this study, we conducted a comprehensive analysis, including microscopic examination of thin sections and the inspection of 60-m cores from five wells, to classify pore types and investigate various forms of karstification in the fifth member of the Majiagou Formation. Our findings have identified distinct pore types, including interparticle pores, separate-vuggy pores, and touching-vuggy pores, in this formation. The dominant forms of karstification in this area were identified as syngenetic karstification, weathering crust karstification, and burial karstification. Importantly, our investigation emphasizes the significant influence of factors such as sea level fluctuations, exposure duration, and the presence of acidic formation water in shaping the observed patterns of karstification. Syngenetic karstification and shallow-buried karstification have emerged as key stages in the formation of natural gas reservoirs. Moreover, our research emphasizes the importance of structural and composite fractures observed in the fifth member of the Majiagou Formation. These unfilled fractures serve as crucial microfracture networks, facilitating oil and gas migration and contributing to the overall development of reservoirs. The research results are of great significance for understanding the formation process of carbonate reservoirs in the Majiagou Formation, Ordos Basin.
Supply-Driven Analysis for a Continuous Water Supply Network Based on a Pressure-Based Outflow at the House Outlets under Peak Withdrawal Scenarios
Authors: Suribabu Conety Ravi , Thurvas Neelakantan Renganathan , Sivakumar Perumal
Published: November 2023

This research brings a new analysis method for a continuous water supply distribution network. The number of house service connections in different story buildings, rather than the nodal peak demand, shall be accounted for in the analysis. This work aims to consider the flow when pipes are opened in the house plumbing systems. The approach deviates from a traditional peak demand-based analysis of the water distribution network. The analysis gives the flow rate at each nodal point that could be observed in the different story buildings. The methodology is applied to a hypothetical network and shows how much flow and nodal pressure can occur when different percentages of consumers are in an active state. This study indicates that the peak demand-based sizing of the supply pipes could have a deficient capacity under real scenarios. The proposed analysis method will help to understand the actual behavior of the network.
The Data Assimilation Approach in a Multilayered Uncertainty Space
Authors: Drieschner Martin , Herrmann Clemens , Petryna Yuri
Published: November 2023

The simultaneous consideration of a numerical model and of different observations can be achieved using data-assimilation methods. In this contribution, the ensemble Kalman filter (EnKF) is applied to obtain the system-state development and also an estimation of unknown model parameters. An extension of the Kalman filter used is presented for the case of uncertain model parameters, which should not or cannot be estimated due to a lack of necessary measurements. It is shown that incorrectly assumed probability density functions for present uncertainties adversely affect the model parameter to be estimated. Therefore, the problem is embedded in a multilayered uncertainty space consisting of the stochastic space, the interval space, and the fuzzy space. Then, we propose classifying all present uncertainties into aleatory and epistemic ones. Aleatorically uncertain parameters can be used directly within the EnKF without an increase in computational costs and without the necessity of additional methods for the output evaluation. Epistemically uncertain parameters cannot be integrated into the classical EnKF procedure, so a multilayered uncertainty space is defined, leading to inevitable higher computational costs. Various possibilities for uncertainty quantification based on probability and possibility theory are shown, and the influence on the results is analyzed in an academic example. Here, uncertainties in the initial conditions are of less importance compared to uncertainties in system parameters that continuously influence the system state and the model parameter estimation. Finally, the proposed extension using a multilayered uncertainty space is applied on a multi-degree-of-freedom (MDOF) laboratory structure: a beam made of stainless steel with synthetic data or real measured data of vertical accelerations. Young’s modulus as a model parameter can be estimated in a reasonable range, independently of the measurement data generation.
Recent Progress in Distiller’s Grains: Chemical Compositions and Biological Activities
Authors: He Ran , Yang Yubo , Li Yongsu , Yang Minghua , Kong Lingyi , Yang Fan
Published: November 2023

Distiller’s grains (DGs) are solid mixtures that remain after the production of alcoholic beverages. A large amount of DGs is produced each year during the brewing process. Currently, they are mostly used as a feedstock or substrate in the feed industry. However, the lack of a comprehensive understanding of the chemical composition of DGs is a major constraint on their further development and application for high-value-added usages. Some studies were published on the bioactive constituents of DGs in several different types of journals. Data were therefore collated to provide a comprehensive overview of these natural products. DGs are rich in phenols, phytosterols, and fatty acids, in addition to general lipid and protein constituents. These compounds and their related extracts possess diverse biological activities, including antioxidant, anti-inflammatory, and anti-hyperglycaemic effects. We hope that this review will provide research incentives for the further development and utilisation of DGs to develop high-value-added products.
Production of Exopolysaccharide-Based Porous Structures for Biomedical Applications: A Review
Authors: Zanotti Alessandra , Baldino Lucia , Reverchon Ernesto
Published: November 2023

Exopolysaccharides, obtained from microorganisms as fermentation products, are interesting candidates for biomedical applications as scaffolds: they are biocompatible, nontoxic, antimicrobial, antitumor materials. To produce exopolysaccharide-based scaffolds, sol–gel technology could be used, which ends with the removal of the liquid phase from the polymeric network (i.e., the drying step). The aim of this review is to point out the most relevant strengths and weaknesses of the different drying techniques, focusing attention on the production of exopolysaccharide-based porous structures. Among these drying processes, supercritical carbon dioxide-assisted drying is the most promising strategy to obtain dried gels to use in the biomedical field: it produces highly porous and lightweight devices with outstanding surface areas and regular microstructure and nanostructure (i.e., aerogels). As a result of the analysis carried out in the present work, it emerged that supercritical technologies should be further explored and applied to the production of exopolysaccharide-based nanostructured scaffolds. Moving research towards this direction, exopolysaccharide utilization could be intensified and extended to the production of high added-value devices.
Sustainable Synthesis of Metal-Doped Lignin-Derived Electrospun Carbon Fibers for the Development of ORR Electrocatalysts
Authors: Jaimes-Paez Cristian Daniel , García-Mateos Francisco José , Ruiz-Rosas Ramiro , Rodríguez-Mirasol José , Cordero Tomás , Morallón Emilia , Cazorla-Amorós Diego
Published: November 2023

The aim of this work is to establish the Oxygen Reduction Reaction (ORR) activity of self-standing electrospun carbon fiber catalysts obtained from different metallic salt/lignin solutions. Through a single-step electrospinning technique, freestanding carbon fiber (CF) electrodes embedded with various metal nanoparticles (Co, Fe, Pt, and Pd), with 8–16 wt% loadings, were prepared using organosolv lignin as the initial material. These fibers were formed from a solution of lignin and ethanol, into which the metallic salt precursors were introduced, without additives or the use of toxic reagents. The resulting non-woven cloths were thermostabilized in air and then carbonized at 900 °C. The presence of metals led to varying degrees of porosity development during carbonization, improving the accessibility of the electrolyte to active sites. The obtained Pt and Pd metal-loaded carbon fibers showed high nanoparticle dispersion. The performance of the electrocatalyst for the oxygen reduction reaction was assessed in alkaline and acidic electrolytes and compared to establish which metals were the most suitable for producing carbon fibers with the highest electrocatalytic activity. In accordance with their superior dispersion and balanced pore size distribution, the carbon fibers loaded with 8 wt% palladium showed the best ORR activity, with onset potentials of 0.97 and 0.95 V in alkaline and acid media, respectively. In addition, this electrocatalyst exhibits good stability and selectivity for the four-electron energy pathway while using lower metal loadings compared to commercial catalysts.
Perfect Absorption and Reflection Modulation Based on Asymmetric Slot-Assisted Gratings without Mirrors
Authors: Lee Sangjun , Kim Sangin
Published: November 2023

As a perfect graphene absorber without any external mirrors, we proposed asymmetric slot-assisted grating structures supporting two degenerate resonant modes of the guided-mode resonances (GMR) and the quasi-bound states in the continuum (quasi-BIC). The GMR mode functions as an internal mirror in conjunction with the background scattering, while the quasi-BIC, which is responsible for perfect graphene absorption, stems from the horizontal symmetry breaking by an asymmetric slot. By properly shifting the slot center from the grating center, the leakage rate of quasi-BIC can be controlled in such a way as to satisfy the critical coupling condition. We provide a comprehensive study on the coupling mechanism of two degenerate resonant modes for a one-port system mimicking the resonance. We also numerically demonstrated that our proposed grating structures show an excellent reflection-type modulation performance at optical wavelength ranges when doped double-layer graphene is applied. Due to the perfect absorption at the OFF state, a high modulation depth of ~50 dB can be achieved via a small Fermi level variation of ~0.05 eV. To obtain the lower insertion loss at the ON state, the higher Fermi level is required to decrease the graphene absorption coefficient.
Simulating Maize Response to Split-Nitrogen Fertilization Using Easy-to-Collect Local Features
Authors: Parent Léon Etienne , Deslauriers Gabriel
Published: November 2023

Maize (Zea mays) is a high-nitrogen (N)-demanding crop potentially contributing to nitrate contamination and emissions of nitrous oxide. The N fertilization is generally split between sowing time and the V6 stage. The right split N rate to apply at V6 and minimize environmental damage is challenging. Our objectives were to (1) predict maize response to added N at V6 using machine learning (ML) models; and (2) cross-check model outcomes by independent on-farm trials. We assembled 461 N trials conducted in Eastern Canada between 1992 and 2022. The dataset to predict grain yield comprised N dosage, weekly precipitations and corn heat units, seeding date, previous crop, tillage practice, soil series, soil texture, organic matter content, and pH. Random forest and XGBoost predicted grain yield accurately at the V6 stage (R2 = 0.78–0.80; RSME and MAE = 1.22–1.29 and 0.96–0.98 Mg ha−1, respectively). Model accuracy up to the V6 stage was comparable to that of the full-season prediction. The response patterns simulated by varying the N doses showed that grain yield started to plateau at 125–150 kg total N ha−1 in eight out of ten on-farm trials conducted independently. There was great potential for economic and environmental gains from ML-assisted N fertilization.
Effect of Extracelluar Vesicles Derived from Akkermansia muciniphila on Intestinal Barrier in Colitis Mice
Authors: Zheng Ting , Hao Haining , Liu Qiqi , Li Jiankun , Yao Yukun , Liu Yisuo , Zhang Tai , Zhang Zhe , Yi Huaxi
Published: November 2023

Inflammatory bowel disease (IBD) is a chronic and recurrent disease. It has been observed that the incidence and prevalence of IBD are increasing, which consequently raises the risk of developing colon cancer. Recently, the regulation of the intestinal barrier by probiotics has become an effective treatment for colitis. Akkermansia muciniphila-derived extracellular vesicles (Akk EVs) are nano-vesicles that contain multiple bioactive macromolecules with the potential to modulate the intestinal barrier. In this study, we used ultrafiltration in conjunction with high-speed centrifugation to extract Akk EVs. A lipopolysaccharide (LPS)-induced RAW264.7 cell model was established to assess the anti-inflammatory effects of Akk EVs. It was found that Akk EVs were able to be absorbed by RAW264.7 cells and significantly reduce the expression of nitric oxide (NO), TNF-α, and IL-1β (p < 0.05). We explored the preventative effects on colitis and the regulating effects on the intestinal barrier using a mouse colitis model caused by dextran sulfate sodium (DSS). The findings demonstrated that Akk EVs effectively prevented colitis symptoms and reduced colonic tissue injury. Additionally, Akk EVs significantly enhanced the effectiveness of the intestinal barrier by elevating the expression of MUC2 (0.53 ± 0.07), improving mucus integrity, and reducing intestinal permeability (p < 0.05). Moreover, Akk EVs increased the proportion of the beneficial bacteria Firmicutes (33.01 ± 0.09%) and downregulated the proportion of the harmful bacteria Proteobacteria (0.32 ± 0.27%). These findings suggest that Akk EVs possess the ability to regulate immune responses, protect intestinal barriers, and modulate the gut microbiota. The research presents a potential intervention approach for Akk EVs to prevent colitis.
Curly Kale (Brassica oleracea var. Sabellica L.) Biofortified with 5,7-Diiodo-8-quinolinol: The Influence of Heat Treatment on Iodine Level, Macronutrient Composition and Antioxidant Content
Authors: Waśniowska Justyna , Leszczyńska Teresa , Kopeć Aneta , Piątkowska Ewa , Smoleń Sylwester , Krzemińska Joanna , Kowalska Iwona , Słupski Jacek , Piasna-Słupecka Ewelina , Krawczyk Katarzyna , Koronowicz Aneta
Published: November 2023

Many disorders are a result of an inadequate supply of macronutrients and micronutrients in the diet. One such element is iodine. This study used curly kale (Brassica oleracea var. Sabellica L.) biofortified with the 5,7-diiodo-8-quinolinol iodine compound. The effect of the heat treatment on the chemical composition of the curly kale was studied. In addition, iodine bioavailability was evaluated in in vivo studies. Our investigation showed that iodine loss depends on the type of heat treatment as well as on the variety of kale. Curly kale biofortified with iodoquinoline had significantly higher iodine levels after thermal processing (steaming, blanching, boiling) than the vegetable biofortified with KIO3. Generally, steaming was the best thermal processing method, as it contributed to the lowest iodine loss in curly kale. The red variety of kale, ‘Redbor F1’, showed a better iodine stability during the heat treatment than the green variety, ‘Oldenbor F1’. The thermal treatment also significantly affected the dry matter content and the basic chemical composition of the tested varieties of the 5,7-diI-8-Q biofortified kale. The steaming process caused a significant increase in total carbohydrates, fiber, protein and crude fat content (‘Oldenbor F1’, ‘Redbor F1’), and antioxidant activity (‘Oldenbor F1’). On the other hand, boiling caused a significant decrease, while steaming caused a significant increase, in protein and dry matter content (‘Oldenbor F1’, ‘Redbor F1’). The blanching process caused the smallest significant decrease in ash compared to the other thermal processes used (‘Oldenbor F1’). A feeding experiment using Wistar rats showed that iodine from the 5,7-diI-8-Q biofortified kale has a higher bioavailability than that from the AIN-93G diet. A number of promising results have been obtained, which could form the basis for further research.
Gut Microbiome-Based Therapeutics in Critically Ill Adult Patients—A Narrative Review
Authors: He Shiyue , Lin Fengyu , Hu Xinyue , Pan Pinhua
Published: November 2023

The gut microbiota plays a crucial role in the human microenvironment. Dysbiosis of the gut microbiota is a common pathophysiological phenomenon in critically ill patients. Therefore, utilizing intestinal microbiota to prevent complications and improve the prognosis of critically ill patients is a possible therapeutic direction. The gut microbiome-based therapeutics approach focuses on improving intestinal microbiota homeostasis by modulating its diversity, or treating critical illness by altering the metabolites of intestinal microbiota. There is growing evidence that fecal microbiota transplantation (FMT), selective digestive decontamination (SDD), and microbiota-derived therapies are all effective treatments for critical illness. However, different treatments are appropriate for different conditions, and more evidence is needed to support the selection of optimal gut microbiota-related treatments for different diseases. This narrative review summarizes the curative effects and limitations of microbiome-based therapeutics in different critically ill adult patients, aiming to provide possible directions for gut microbiome-based therapeutics for critically ill patients such as ventilator-associated pneumonia, sepsis, acute respiratory distress syndrome, and COVID-19, etc.
Food Intake and Sleep Disorders in Children and Adolescents with Obesity
Authors: Calcaterra Valeria , Rossi Virginia , Tagi Veronica Maria , Baldassarre Paola , Grazi Roberta , Taranto Silvia , Zuccotti Gianvincenzo
Published: November 2023

Over the last few decades, numerous scientific studies have investigated the possible association between sleep duration and adiposity during childhood, since it has been reported that sleep deprivation causes a related increase in caloric intake. Even though the underlying pathogenetic mechanisms are still under study and not completely known, the effect of dietetic habits and nutrient intake on sleep quality and patterns has been reported. The aim of this study is to explore the intricate interplay between food intake/diet patterns and pediatric sleep disturbances in children and adolescents with obesity, emphasizing the importance of not underestimating this aspect in the prevention and treatment of this complex disease. Recent evidence supports a high correlation between specific diet patterns and foods with sleep disturbances in children at all ages. Diets rich in fiber, fruit, vegetables, and anti-inflammatory nutrients and low in saturated fats seem to promote better sleep quality. Sleep disturbances are, in turn, risk factors for the development of obesity. Therefore, food strategies should be applied to counteract this harmful process. Unraveling the complex links between dietary habits, sleep patterns, and obesity is essential for developing effective strategies to combat this critical public health issue.
The Efficacy and Safety of Biologic Drugs in the Treatment of Moderate–Severe Crohn’s Disease: A Systematic Review
Authors: Avedillo-Salas Ana , Corral-Cativiela Sara , Fanlo-Villacampa Ana , Vicente-Romero Jorge
Published: November 2023

Conventional therapy is the most commonly used treatment for Crohn’s disease (CD), but it does not always achieve disease control, which is why the use of biologic drugs is increasing. The aim of this study was to analyze the efficacy and safety of biologic drugs in adult patients diagnosed with moderate–severe CD. An intensive search was performed in PubMed, Web of Science and Medline to collect phase 2 or 3 clinical trials published between 2018 and 2023 that were randomized, placebo-controlled and double-blind trials analyzing the efficacy and safety of biologic drugs in adult patients diagnosed with CD. This systematic review was conducted according to the PRISMA statement. Thirteen clinical trials evaluating eight biologic drugs were included. Upadacitinib, vedolizumab, adalimumab, guselkumab, mirikizumab, ustekinumab and risankizumab showed statistically significant efficacy across different clinical, endoscopic, histological, genetic, biomarker or quality-of-life parameters. However, PF-00547659 only showed statistically significant results for the CDAI-70 at week 12. In terms of safety, the incidence and severity of adverse effects were analyzed, with all drugs being well tolerated and presenting a good safety profile since most adverse effects were mild. Biologic drugs can be considered an effective and safe option for the treatment of moderate–severe CD in adult patients with an inadequate response or intolerance to conventional therapy.
Carborane-Based ABCG2-Inhibitors Sensitize ABC-(Over)Expressing Cancer Cell Lines for Doxorubicin and Cisplatin
Authors: Paskas Svetlana , Stockmann Philipp , Mijatović Sanja , Kuhnert Lydia , Honscha Walther , Hey-Hawkins Evamarie , Maksimović-Ivanić Danijela
Published: November 2023

The ABCG2 transporter protein, as part of several known mechanisms involved in multidrug resistance, has the ability to transport a broad spectrum of substrates out of the cell and is, therefore, considered as a potential target to improve cancer therapies or as an approach to combat drug resistance in cancer. We have previously reported carborane-functionalized quinazoline derivatives as potent inhibitors of human ABCG2 which effectively reversed breast cancer resistance protein (BCRP)-mediated mitoxantrone resistance. In this work, we present the evaluation of our most promising carboranyl BCRP inhibitors regarding their toxicity towards ABCG2-expressing cancer cell lines (MCF-7, doxorubicin-resistant MCF-7 or MCF-7 Doxo, HT29, and SW480) and, consequently, with the co-administration of an inhibitor and therapeutic agent, their ability to increase the efficacy of therapeutics with the successful inhibition of ABCG2. The results obtained revealed synergistic effects of several inhibitors in combination with doxorubicin or cisplatin. Compounds DMQCa, DMQCc, and DMQCd showed a decrease in IC50 value in ABCB1- and ABCG2-expressing SW480 cells, suggesting a possible targeting of both transporters. In an HT29 cell line, with the highest expression of ABCG2 among the tested cell lines, using co-treatment of doxorubicin and DMQCd, the effective inhibitory concentration of the antineoplastic agent could be reduced by half. Interestingly, co-treatment of compound QCe with cisplatin, which is not an ABCG2 substrate, showed synergistic effects in MCF-7 Doxo and HT29 cells (IC50 values halved or reduced by 20%, respectively). However, a literature-known upregulation of cisplatin-effluxing ABC transporters and their effective inhibition by the carborane derivatives emerges as a possible reason.
Comparative Analysis of the Physicochemical and Biological Characteristics of Freeze-Dried PEGylated Cationic Solid Lipid Nanoparticles
Authors: Narváez-Narváez David A. , Duarte-Ruiz María , Jiménez-Lozano Sandra , Moreno-Castro Cristina , Vargas Ronny , Nardi-Ricart Anna , García-Montoya Encarna , Pérez-Lozano Pilar , Suñé-Negre Josep Mª , Hernández-Munain Cristina , Suñé Carlos , Suñé-Pou Marc
Published: November 2023

Cationic solid-lipid nanoparticles (cSLNs) have become a promising tool for gene and RNA therapies. PEGylation (PEG) is crucial in enhancing particle stability and protection. We evaluated the impact of PEG on the physicochemical and biological characteristics of cholesteryl-oleate cSLNs (CO-cSLNs). Several parameters were analyzed, including the particle size, polydispersity index, zeta potential, shape, stability, cytotoxicity, and loading efficiency. Five different formulations with specific PEGs were developed and compared in both suspended and freeze-dried states. Small, homogeneous, and cationic suspended nanoparticles were obtained, with the Gelucire 50/13 (PEG-32 hydrogenated palm glycerides; Gelucire) and DSPE-mPEG2000 (1,2-distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjungate-2000; DSPE) formulations exhibiting the smallest particle size (~170 nm). Monodisperse populations of freeze-dried nanoparticles were also achieved, with particle sizes ranging from 200 to 300 nm and Z potential values of 30–35 mV. Notably, Gelucire again produced the smallest particle size (211.1 ± 22.4), while the DSPE and Myrj S100 (polyoxyethylene (100) stearate; PEG-100 Stearate) formulations had similar particle sizes to CO-cSLNs (~235 nm). The obtained PEGylated nanoparticles showed suitable properties: they were nontoxic, had acceptable morphology, were capable of forming SLNplexes, and were stable in both suspended and lyophilized states. These PEG-cSLNs are a potential resource for in vivo assays and have the advantage of employing cost-effective PEGs. Optimizing the lyophilization process and standardizing parameters are also recommended to maintain nanoparticle integrity.
A Pharmacovigilance Study Regarding the Risk of Antibiotic-Associated Clostridioides difficile Infection Based on Reports from the EudraVigilance Database: Analysis of Some of the Most Used Antibiotics in Intensive Care Units
Authors: Vintila Bogdan Ioan , Arseniu Anca Maria , Morgovan Claudiu , Butuca Anca , Sava Mihai , Bîrluțiu Victoria , Rus Luca Liviu , Ghibu Steliana , Bereanu Alina Simona , Roxana Codru Ioana , Gligor Felicia Gabriela
Published: November 2023

The Gram-positive anaerobic bacterium Clostridioides difficile (CD) can produce intense exotoxins, contributing to nosocomial infections, and it is the most common cause of health-care-associated infectious diarrhea. Based on spontaneous Individual Case Safety Reports from EudraVigilance (EV), we conducted a descriptive analysis of Clostridioides difficile infection (CDI) cases that reported a spontaneous adverse reaction related to using ceftriaxone, colistimethate, ciprofloxacin, gentamicin, linezolid, meropenem, and piperacillin/tazobactam. Most ADR reports registered in EV that were related to CDI were associated with ceftriaxone (33%), ciprofloxacin (28%), and piperacillin/tazobactam (21%). Additionally, the disproportionality analysis performed showed that all studied antibiotics had a lower reporting probability when compared to clindamycin. A causal relationship between a drug and the occurrence of an adverse reaction cannot be established from EV data alone because the phenomena of underreporting, overreporting, and reporting bias may affect the results. Based on the analysis of the collected data, this study underlines the importance of surveillance and monitoring programs for the consumption of antibiotics. Furthermore, it is essential to use standardized laboratory tests to define CDI’s nature accurately. To prevent this infection, specialists should collaborate and adhere strictly to antibiotic stewardship programs, hygiene practices, and isolation protocols.
Rubia cordifolia L. Attenuates Diabetic Neuropathy by Inhibiting Apoptosis and Oxidative Stress in Rats
Authors: Bana Sweeti , Kumar Nitin , Sartaj Ali , Alhalmi Abdulsalam , Qurtam Ashraf Ahmed , Nasr Fahd A. , Al-Zharani Mohammed , Singh Neelam , Gaur Praveen , Mishra Rosaline , Bhardwaj Snigdha , Ali Hasan , Goel Radha
Published: November 2023

Background: Diabetic neuropathy is a debilitating manifestation of long-term diabetes mellitus. The present study explored the effects of the roots of Rubia cordifolia L. (R. cordifolia L.) in the Wistar rat model for diabetic neuropathy and possible neuroprotective, antidiabetic, and analgesic mechanisms underlying this effect. Materials and Methods: Rats were divided into five experimental groups. An amount of 0.25% carboxy methyl cellulose (CMC) in saline and streptozotocin (STZ) (60 mg/kg) was given to group 1 and group 2, respectively. Group 3 was treated with STZ and glibenclamide simultaneously while groups 4 and 5 were simultaneously treated with STZ and hydroalcoholic extract of the root of R. cordifolia, respectively. Hot plate and cold allodynias were used to evaluate the pain threshold. The antioxidant effects of R. cordifolia were assessed by measuring Thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD). At the end of the study, sciatic nerve and brain tissues were collected for histopathological study. Bcl-2 proteins, cleaved caspase-3, and Bax were assessed through the Western blot method. Results: R. cordifolia significantly attenuated paw withdrawal and tail flick latency in diabetic neuropathic rats. R. cordifolia significantly (p < 0.01) improved the levels of oxidative stress. It was found to decrease blood glucose levels and to increase animal weight in R. cordifolia-treated groups. Treatment with R. cordifolia suppressed the cleaved caspase-3 and reduced the Bax:Bcl2 ratio in sciatic nerve and brain tissue compared to the diabetic group. Histopathological analysis also revealed a marked improvement in architecture and loss of axons in brain and sciatic nerve tissues at a higher dose of R. cordifolia (400 mg/kg). Conclusion: R. cordifolia attenuated diabetic neuropathy through its antidiabetic and analgesic properties by ameliorating apoptosis and oxidative stress.
Polymers Enhancing Bioavailability in Drug Delivery, 2nd Edition
Authors: Fernandes Ana I.
Published: November 2023

This Special Issue continues the previously published work [...]
Alginate-Based Hydrogels Enriched with Lavender Essential Oil: Evaluation of Physicochemical Properties, Antimicrobial Activity, and In Vivo Biocompatibility
Authors: Rusu Alina Gabriela , Niță Loredana Elena , Roșca Irina , Croitoriu Alexandra , Ghilan Alina , Mititelu-Tarțău Liliana , Grigoraș Aurica Valentin , Crețu Bianca-Elena-Beatrice , Chiriac Aurica P.
Published: November 2023

Owing to its antibacterial, anti-inflammatory, and antioxidant activities, in the last few years, lavender essential oil (LVO) has been used in medical applications as a promising approach for treating infected wounds. However, the practical applicability of LVO is limited by its high volatility and storage stability. This study aimed to develop a novel hybrid hydrogel by combining phytic acid (PA)-crosslinked sodium alginate (SA) and poly(itaconic anhydride-co-3,9-divinyl-2,4,8,10-tetraoxaspiro[5.5] undecane (PITAU) and evaluate its potential effectiveness as an antibacterial wound dressing after incorporating LVO. The influence of the mass ratio between SA and PITAU on the properties and stability of hydrogels was investigated. After LVO loading, the effect of oil addition to hydrogels on their functional properties and associated structural changes was studied. FTIR analysis revealed that hydrogen bonding is the primary interaction mechanism between components in the hybrid hydrogels. The morphology was analyzed using SEM, evidencing a porosity dependent on the ratio between SA and PITAU, while LVO droplets were well dispersed in the polymer blend. The release of LVO from the hydrogels was determined using UV-VIS spectroscopy, indicating a sustained release over time, independent of the LVO concentration. In addition, the hybrid hydrogels were tested for their antioxidant properties and antimicrobial activity against Gram-positive and Gram-negative bacteria. Very good antimicrobial activity was obtained in the case of sample SA_PITAU3+LVO10% against S. aureus and C. albicans. Moreover, in vivo tests showed an increased antioxidant effect of the SA_PITAU3+LVO10% hydrogel compared to the oil-free scaffold that may aid in accelerating the healing process of wounds.