bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex before the State: Civic Sex, Reproductive Innovations, and Gendered Parental Identity
Authors: MURPHY TIMOTHY F.
Published: March 2017

Certain changes in the way that states classify people by sex as well as certain reproductive innovations undercut the rationale for state identification of people as male or female in signifying gendered parental relationships to children. At present, people known to the state as men may be genetic mothers to their children; people known to the state as women may be genetic fathers to their children. Synthetic gametes would make it possible for transgender men to be genetically related to children as fathers and transgender women to be genetically related to children as mothers, even if they have otherwise relied on naturally-occurring gametes to be genetic mothers and genetic fathers of children respectively. Synthetic gametes would presumably make it possible for any person to be the genetic father or genetic mother of children, even in a mix-and-match way. Other reproductive innovations will also undercut existing expectations of gendered parental identity. Uterus transplants would uncouple the maternal function of gestation from women, allowing men to share in maternity that way. Extracorporeal gestation ((ExCG)—gestation outside anyone’s body—would also undercut the until-now absolute connection between female sex and maternity. In kind, effects such as these—undoing conventionally gendered parenthood—undercut the state’s interest in knowing whether parents are male or female in relation to a given child, as against knowing simply whether someone stands in a parental relationship to that child, as a matter of rights and duties.
Health Information Technology as a Universal Donor to Bioethics Education
Authors: GOODMAN KENNETH W.
Published: March 2017

Health information technology, sometimes called biomedical informatics, is the use of computers and networks in the health professions. This technology has become widespread, from electronic health records to decision support tools to patient access through personal health records. These computational and information-based tools have engendered their own ethics literature and now present an opportunity to shape the standard medical and nursing ethics curricula. It is suggested that each of four core components in the professional education of clinicians—privacy, end-of-life care, access to healthcare and valid consent, and clinician–patient communication—offers an opportunity to leverage health information technology for curricular improvement. Using informatics in ethics education freshens ethics pedagogy and increases its utility, and does so without additional demands on overburdened curricula.
Legitimacy and the UN Security Council Agenda
Authors: Frederking Brian , Patane Christopher
Published: March 2017

Is the United Nations Security Council (UNSC) a legitimate organization? Do the veto powers legitimately pursue international security, or do they protect their narrow national interests? One way to evaluate the legitimacy of the UNSC is through its agenda. Does it address the most significant conflicts in world politics? Or is it influenced by the national interests of the veto powers? This article addresses these questions with a dataset that includes the number of UNSC meetings held and resolutions passed on 40 conflicts from 1991 to 2013. This analysis provides evidence for the legitimacy of the UNSC—conflicts with more refugees and more deaths are significantly more likely to be on the agenda. The analysis does not support critics of the UNSC—the national interests of the veto powers, measured as arms sales to and trade with the conflict participants, do not significantly alter the UNSC agenda.
Going Beyond the Existing Consensus: The Use of Games in International Relations Education
Authors: Lee Michael , Shirkey Zachary C.
Published: March 2017

Despite the popularity of using games to teach international relations, few works directly assess their effectiveness. Furthermore, it is unclear if games help all students equally, or if certain students are more likely to benefit than others. Finally, how closely the game must mirror the concept being taught to be an effective pedagogical tool has received scant attention. We address these points by discussing the use of an updated version of the classic American election game, Consensus, to help illustrate the role of domestic political coalitions in an international political economy course. Assessing the performance of 39 students via a pre- and post-quiz, we find that student performance improved overall, particularly among frequent gamers.
Ages and chemical compositions of massive clusters in NGC147 and M31
Authors: Sharina Margarita , Shimansky Vladislav
Published: March 2017

We present estimates of ages, [Fe/H], helium content (Y) and abundances of C, N, Mg, Ca, and several other elements for the following globular clusters (GCs): GC7 in NGC147, and Mayall II, Mackey 1 and Mackey 6 in M31. Medium-resolution integrated-light spectra of the GCs were conducted with the 6m telescope. To derive the ages and abundances for the GCs we carried out their population synthesis using model stellar atmospheres, the Padova YZVAR isochrones and the Chabrier mass function. We compare the results with the corresponding data obtained using the same method for several massive Galactic GCs. We show that the differences in the light-element abundances between GCs with similar ages and metallicities may reach 0.5-0.6 dex. The corresponding differences for other elements are usually 2-3 times smaller. We suggest that at least partially the detected differences may be due to light-element abundance variations in the atmospheres of high-luminosity red giant branch stars as a consequence of the transportation of the produced elements to the surface layers.
Theoretical evolutionary framework for low-mass stars in massive star clusters
Authors: Cassisi Santi
Published: March 2017

Recent spectroscopic and photometric surveys of Galactic Globular Clusters have shown that these stellar systems host distinct sub-populations of stars characterised by peculiar chemical patterns. In the following we wish to address the issue of how these specific chemical patterns affect both the structural and evolutionary properties of stars as well as their spectral energy distribution. The implications of these effects on the photometric appearance of multiple stellar populations in different photometric planes are also briefly discussed.
Accretion onto Protoplanetary Discs: Implications for Globular Cluster Evolution
Authors: Wijnen T. P. G. , Pols O. R. , Pelupessy F. I. , Zwart S. Portegies
Published: March 2017

In the past decade, observational evidence that Globular Clusters (GCs) harbour multiple stellar populations has grown steadily. These observations are hard to reconcile with the classical picture of star formation in GCs, which approximates them as a single generation of stars. Bastian et al. recently suggested an evolutionary scenario in which a second, chemically distinct, population is formed by the accretion of chemically enriched material onto the protoplanetary disc of low-mass stars in the initial GC population. Using assumptions that represent the (dynamical) conditions in a typical GC, we investigate whether a low-mass star surrounded by a protoplanetary disc can accrete sufficient enriched material to account for the observed abundances in ‘second generation’ stars. We compare the outcome of two different smoothed particle hydrodynamics codes and focus on the lifetime and stability of the disc and on the gas accretion rate onto both the star and the disc.
Initial conditions of formation of starburst clusters: constraints from stellar dynamics
Authors: Banerjee Sambaran
Published: March 2017

How starburst clusters form out of molecular clouds is still an open question. In this article, I highlight some of the key constraints in this regard, that one can get from the dynamical evolutionary properties of dense stellar systems. I particularly focus on secular expansion of massive star clusters and hierarchical merging of sub-clusters, and discuss their implicationsvis-á-visthe observed properties of young massive clusters. The analysis suggests that residual gas expulsion is necessary for shaping these clusters as we see them today, irrespective of their monolithic or hierarchical mode of formation.
A critical assessment of models for the origin of multiple populations in globular clusters
Authors: Bastian Nate
Published: March 2017

A number of scenarios have been put forward to explain the origin of the chemical anomalies (and resulting complex colour-magnitude diagrams) observed in globular clusters (GCs), namely the AGB, Fast Rotating Massive Star, Very Massive Star, and Early Disc Accretion scenarios. We compare the predictions of these scenarios with a range of observations (including young massive clusters (YMCs), chemical patterns, and GC population properties) and find that all models are inconsistent with observations. In particular, YMCs do not show evidence for multiple epochs of star-formation and appear to be gas free by an age of ~ 3 Myr. Also, the chemical patterns displayed in GCs vary from one to the next in such a way that cannot be reproduced by standard nucleosynthetic yields. Finally, we show that the “mass budget problem” for the scenarios cannot be solved by invoking heavy cluster mass loss (i.e. that clusters were 10-100 times more massive at birth) as this solution makes basic predictions about the GC population that are inconsistent with observations. We conclude that none of the proposed scenarios can explain the multiple population phenomenon, hence alternative theories are needed.
The Initial Mass Function of Young Open Clusters in the Galaxy: A Preliminary Result
Authors: Lim Beomdu , Sung Hwankyung , Hur Hyeonoh , Park Byeong-Gon
Published: March 2017

The initial mass function (IMF) is an essential tool with which to study star formation processes. We have initiated the photometric survey of young open clusters in the Galaxy, from which the stellar IMFs are obtained in a homogeneous way. A total of 16 famous young open clusters have preferentially been studied up to now. These clusters have a wide range of surface densities (log σ = −1 to 3 [stars pc−2] for stars with mass larger than 5M⊙) and cluster masses (Mcl = 165 to 50, 000M⊙), and also are distributed in five different spiral arms in the Galaxy. It is possible to test the dependence of star formation processes on the global properties of individual clusters or environmental conditions. We present a preliminary result on the variation of the IMF in this paper.
Multiple populations in globular clusters: constraints from kinematics and dynamics
Authors: Hénault-Brunet Vincent
Published: March 2017

We discuss constraints on the formation of multiple populations in globular clusters (GCs) imposed by their present-day kinematics (velocity dispersion and anisotropy) and spatial distribution. We argue that the observational evidence collected so far in the outer parts of clusters is generally consistent with an enriched population forming more centrally concentrated compared to the primordial population, in agreement with all the scenarios proposed to date (in some cases by design), but not sufficient to favour a particular scenario. We highlight that the differential rotation of subpopulations is a signature that may provide crucial new constraints and allow us to distinguish between various scenarios. Finally, we discuss the spatial distribution of subpopulations in the central regions of GCs and speculate that mass segregation between subpopulations may be due to a difference in their binary fraction.
Formation of the twin galactic starburst regions NGC 6357 and NGC 6334
Authors: Torii Kazufumi , Hasegawa Keisuke , Ohama Akio , Yamamoto Hiroaki , Tachihara Kengo , Fukui Yasuo
Published: March 2017

New CO J=1–0 observations with NANTEN and NANTEN2 reveal that extensive collisions between two molecular clouds at relative velocity of 15 km s−1 triggered the O star formation in the Galactic mini-starbursts NGC 6357 and NGC 6334. Correlated/anti-correlated gas distributions and intermediate velocity features between the two clouds lend support for the cloud-cloud collision scenario. The timescale of the collision and high-mass star formation is as short as less than 0.5 Myrs, suggesting rapid O star formation.
Twins born in different environments? Nuclei of two dSphs: isolated galaxy KKS3 and E269-66, a close neighbor of NGC5128
Authors: Sharina Margarita , Kniazev Alexei , Karachentsev Igor
Published: March 2017

We present the results of age, metallicity and radial velocity determination for central massive globular clusters (GCs) in dwarf spheroidal galaxies: KKs3 and ESO269-66. KKS3 is a unique isolated galaxy. ESO269-66 is a close neighbor of the giant S0 Centaurus A. The results contribute to the knowledge about the origin of massive star clusters and their host dSphs. The structure and star formation histories of the two dwarf galaxies look rather similar. Both of them have experienced several star-forming events. The most recent ones occurred 1-2 Gyr ago, and most powerful bursts happened 12-14 Gyrs ago. Our analysis has shown that both GCs appear to be 1-2 Gyr younger and 0.1-0.3 dex more metal-rich than the most ancient metal-poor stars in the host dSphs. We examine signatures of multiple stellar population in the GCs using our data. Since central star-forming bursts were extended in time, the massive clusters might be considered as nuclei of the galaxies.
Non-thermal emission in Cyg OB2
Authors: Fenech D. , Morford J. , Prinja R.
Published: March 2017

We report here on the first results from the Cyg OB2 Radio Survey (COBRaS), which is a UCL-led e-MERLIN legacy project to provide a deep-field radio mapping of the Cygnus OB2 association. The project has been awarded a total allocation of 252 hours at C-band (5 GHz) and 42 hours at L-band (1.6 GHz) to image the core of the cluster.

We discuss in particular the presence of non-thermal radio emission at 20 cm (L-band), and its potential as a highly efficient way to identify binaries via single-epoch observations, particularly for colliding-wind binaries. COBRaS data will provide a powerful tool for establishing binary incidence in Cyg OB2, specifically in the difficult intermediate-period range (1–100 yr).

Ultimately, we aim to assemble a substantial and uniquely sensitive radio dataset, which will be exploited to address several fundamentally important areas of stellar astrophysics, including mass-loss, binary frequency, stellar cluster dynamics, and triggered star-formation.
Iapetus's Ridge: The Result of Many Small Impacts?
Authors: Wendel JoAnna
Published: March 2017

A ridge around Saturn's third-largest moon has scientists scratching their heads.
A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour
Authors: Huang Lu , Yuan Tifei , Tan Minjie , Xi Yue , Hu Yu , Tao Qian , Zhao Zhikai , Zheng Jiajun , Han Yushui , Xu Fuqiang , Luo Minmin , Sollars Patricia J. , Pu Mingliang , Pickard Gary E. , So Kwok-Fai , Ren Chaoran
Published: March 2017

Animals promote their survival by avoiding rapidly approaching objects that indicate threats. In mice, looming-evoked defensive responses are triggered by the superior colliculus (SC) which receives direct retinal inputs. However, the specific neural circuits that begin in the retina and mediate this important behaviour remain unclear. Here we identify a subset of retinal ganglion cells (RGCs) that controls mouse looming-evoked defensive responses through axonal collaterals to the dorsal raphe nucleus (DRN) and SC. Looming signals transmitted by DRN-projecting RGCs activate DRN GABAergic neurons that in turn inhibit serotoninergic neurons. Moreover, activation of DRN serotoninergic neurons reduces looming-evoked defensive behaviours. Thus, a dedicated population of RGCs signals rapidly approaching visual threats and their input to the DRN controls a serotonergic self-gating mechanism that regulates innate defensive responses. Our study provides new insights into how the DRN and SC work in concert to extract and translate visual threats into defensive behavioural responses.
Graphene-like nanoribbons periodically embedded with four- and eight-membered rings
Authors: Liu Meizhuang , Liu Mengxi , She Limin , Zha Zeqi , Pan Jinliang , Li Shichao , Li Tao , He Yangyong , Cai Zeying , Wang Jiaobing , Zheng Yue , Qiu Xiaohui , Zhong Dingyong
Published: March 2017

Embedding non-hexagonal rings into sp2-hybridized carbon networks is considered a promising strategy to enrich the family of low-dimensional graphenic structures. However, non-hexagonal rings are energetically unstable compared to the hexagonal counterparts, making it challenging to embed non-hexagonal rings into carbon-based nanostructures in a controllable manner. Here, we report an on-surface synthesis of graphene-like nanoribbons with periodically embedded four- and eight-membered rings. The scanning tunnelling microscopy and atomic force microscopy study revealed that four- and eight-membered rings are formed between adjacent perylene backbones with a planar configuration. The non-hexagonal rings as a topological modification markedly change the electronic properties of the nanoribbons. The highest occupied and lowest unoccupied ribbon states are mainly distributed around the eight- and four-membered rings, respectively. The realization of graphene-like nanoribbons comprising non-hexagonal rings demonstrates a controllable route to fabricate non-hexagonal rings in nanoribbons and makes it possible to unveil their unique properties induced by non-hexagonal rings.
Petri Net computational modelling of Langerhans cell Interferon Regulatory Factor Network predicts their role in T cell activation
Authors: Polak Marta E. , Ung Chuin Ying , Masapust Joanna , Freeman Tom C. , Ardern-Jones Michael R.
Published: March 2017

Langerhans cells (LCs) are able to orchestrate adaptive immune responses in the skin by interpreting the microenvironmental context in which they encounter foreign substances, but the regulatory basis for this has not been established. Utilising systems immunology approaches combining in silico modelling of a reconstructed gene regulatory network (GRN) with in vitro validation of the predictions, we sought to determine the mechanisms of regulation of immune responses in human primary LCs. The key role of Interferon regulatory factors (IRFs) as controllers of the human Langerhans cell response to epidermal cytokines was revealed by whole transcriptome analysis. Applying Boolean logic we assembled a Petri net-based model of the IRF-GRN which provides molecular pathway predictions for the induction of different transcriptional programmes in LCs. In silico simulations performed after model parameterisation with transcription factor expression values predicted that human LC activation of antigen-specific CD8 T cells would be differentially regulated by epidermal cytokine induction of specific IRF-controlled pathways. This was confirmed by in vitro measurement of IFN-γ production by activated T cells. As a proof of concept, this approach shows that stochastic modelling of a specific immune networks renders transcriptome data valuable for the prediction of functional outcomes of immune responses.
Social cognition in aggressive offenders: Impaired empathy, but intact theory of mind
Authors: Winter Korina , Spengler Stephanie , Bermpohl Felix , Singer Tania , Kanske Philipp
Published: March 2017

Aggressive, violent behaviour is a major burden and challenge for society. It has been linked to deficits in social understanding, but the evidence is inconsistent and the specifics of such deficits are unclear. Here, we investigated affective (empathy) and cognitive (Theory of Mind) routes to understanding other people in aggressive individuals. Twenty-nine men with a history of legally relevant aggressive behaviour (i.e. serious assault) and 32 control participants were tested using a social video task (EmpaToM) that differentiates empathy and Theory of Mind and completed questionnaires on aggression and alexithymia. Aggressive participants showed reduced empathic responses to emotional videos of others’ suffering, which correlated with aggression severity. Theory of Mind performance, in contrast, was intact. A mediation analysis revealed that reduced empathy in aggressive men was mediated by alexithymia. These findings stress the importance of distinguishing between socio-affective and socio-cognitive deficits for understanding aggressive behaviour and thereby contribute to the development of more efficient treatments.
Evaluation of Direct Haptic 4D Volume Rendering of Partially Segmented Data for Liver Puncture Simulation
Authors: Mastmeyer Andre , Fortmeier Dirk , Handels Heinz
Published: March 2017

This work presents an evaluation study using a force feedback evaluation framework for a novel direct needle force volume rendering concept in the context of liver puncture simulation. PTC/PTCD puncture interventions targeting the bile ducts have been selected to illustrate this concept. The haptic algorithms of the simulator system are based on (1) partially segmented patient image data and (2) a non-linear spring model effective at organ borders. The primary aim is to quantitatively evaluate force errors caused by our patient modeling approach, in comparison to haptic force output obtained from using gold-standard, completely manually-segmented data. The evaluation of the force algorithms compared to a force output from fully manually segmented gold-standard patient models, yields a low mean of 0.12 N root mean squared force error and up to 1.6 N for systematic maximum absolute errors. Force errors were evaluated on 31,222 preplanned test paths from 10 patients. Only twelve percent of the emitted forces along these paths were affected by errors. This is the first study evaluating haptic algorithms with deformable virtual patients in silico. We prove haptic rendering plausibility on a very high number of test paths. Important errors are below just noticeable differences for the hand-arm system.
A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
Authors: Ho Yan Teck , Adriani Giulia , Beyer Sebastian , Nhan Phan-Thien , Kamm Roger D. , Kah James Chen Yong
Published: March 2017

The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (Pd) of NPs is difficult with in vivo models. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeability in vitro based on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the Pd of the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their fluorescence intensity when diffusing across the monolayer. Using 70 kDa dextran as a probe, untreated HUVECs yielded a Pd that approximated tumor vasculature while HUVECs treated with 25 μg/mL cAMP had Pd that approximated healthy vasculature in vivo. As the size of pNPs increased, its Pd decreased in tumor vasculature, but remained largely unchanged in healthy vasculature, demonstrating a trend similar to tumor selectivity for smaller NPs. This microfluidic model of vascular-tissue interface can be used in any laboratory to perform quantitative assessment of the tumor selectivity of nanomedicine-based systems.
Quantitative humoral profiling of the HIV-1 proteome in elite controllers and patients with very long-term efficient antiretroviral therapy
Authors: Zhang Wang , Morshed Mohammed M. , Noyan Kajsa , Russom Aman , Sönnerborg Anders , Neogi Ujjwal
Published: March 2017

A major challenge in evaluating the success of HIV eradication approaches is the need for accurate measurement of persistent HIV during effective antiretroviral therapy (ART). Previous studies have reported that the anti-HIV antibody assay “luciferase immuno-precipitation systems (LIPS)” can distinguish HIV-infected individuals harboring different sizes of the viral reservoirs. We performed antibody profiling of HIV-1 proteomes using LIPS in viremic progressors (n = 38), elite controllers (ECs; n = 19) and patients with fully suppressive long-term antiretroviral therapy (ART) (n = 19) (mean 17 years). IgG was quantified against six HIV-1 fusion proteins: p24, gp41, RT, Tat, integrase and protease. Lower antibody levels to all six-fusion proteins were observed in long-term ART patients compared to viremics (p < 0.05). In contrast ECs had lower antibody levels only against Tat and Integrase (p < 0.05). Principal component analysis and cluster-network analysis identified that 68% (13/19) of the long-term ART patients clustered together with 26% (5/19) ECs. The remaining ECs clustered together with the viremics indicating non-homogeneity among the ECs. The low anti-HIV levels in the long-term treated patients may indicate a restricted remaining viral replication. In contrast, the higher levels in ECs suggest a continuous viral expression with a limited concomitant release of extracellular virus.
Hydroxyethyl cellulose matrix applied to serial crystallography
Authors: Sugahara Michihiro , Nakane Takanori , Masuda Tetsuya , Suzuki Mamoru , Inoue Shigeyuki , Song Changyong , Tanaka Rie , Nakatsu Toru , Mizohata Eiichi , Yumoto Fumiaki , Tono Kensuke , Joti Yasumasa , Kameshima Takashi , Hatsui Takaki , Yabashi Makina , Nureki Osamu , Numata Keiji , Nango Eriko , Iwata So
Published: March 2017

Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.
Meteorological fluctuations define long-term crop yield patterns in conventional and organic production systems
Authors: Teasdale John R. , Cavigelli Michel A.
Published: March 2017

Variability in meteorological patterns presents significant challenges to crop production consistency and yield stability. Meteorological influences on corn and soybean grain yields were analyzed over an 18-year period at a long-term experiment in Beltsville, Maryland, U.S.A., comparing conventional and organic management systems. Precipitation and temperature variables explained much of the yield variability, with precipitation and heat stress during the late vegetative and early reproductive phases of crop growth accounting for the majority of yield variability in all crops and management systems. Crop yields under conventional and organic management followed similar periodic patterns, but yields were 31% and 20% lower in organic than conventional corn and soybean, respectively. The efficiency of grain yield per unit precipitation was higher under conventional than organic management, highlighting the importance of crop management for optimizing production in response to meteorological variability. Periodic yield and precipitation patterns did not consistently align with global meteorological cycles such as the El Niño Southern Oscillation.
Changes in Hepatobiliary Enzyme Abnormality After the Great East Japan Earthquake: The Fukushima Health Management Survey
Authors: Takahashi Atsushi , Ohira Tetsuya , Uemura Mayu , Hosoya Mitsuaki , Yasumura Seiji , Hashimoto Shigeatsu , Ohira Hiromasa , Sakai Akira , Ohtsuru Akira , Satoh Hiroaki , Kawasaki Yukihiko , Suzuki Hitoshi , Sugiura Yoshihiro , Shishido Hiroaki , Hayashi Yoshimitsu , Takahashi Hideto , Nakano Hironori , Kobashi Gen , Ozasa Kotaro , Ohto Hitoshi , Abe Masafumi
Published: March 2017

Although the incidence of hepatobiliary enzyme abnormality increased immediately after the Great East Japan Earthquake and subsequent Fukushima Daiichi Nuclear Power Plant accident, longer-term trends remain unclear. The aims of this study were to determine longer-term trends in hepatobiliary enzyme abnormality and to elucidate lifestyle factors associated with such changes among residents of a nuclear-disaster-affected area. This longitudinal survey enrolled 20,395 adults living in the vicinity of Fukushima Daiichi Nuclear Power Plant. Data were obtained from the records of annual health checkups of adults aged ≥40 years between 2011 and 2012. Follow-up examinations were conducted from June 2013 to March 2014. Associations were assessed between changes in hepatobiliary enzyme abnormality immediately and 3–4 years after the disaster and lifestyle factors. The overall prevalence of hepatobiliary enzyme abnormality significantly decreased over the study period, from 29.9% to 27.1%. Multivariate logistic regression analysis revealed significant associations between improved hepatobiliary enzyme abnormality and improvements in daily physical activity and frequency of breakfast consumption. The results suggest that improvements in daily physical activity and frequency of breakfast consumption significantly reduced the incidence of hepatobiliary enzyme abnormality 3–4 years after the Great East Japan Earthquake and Fukushima Daiichi Nuclear Power Plant accident.
Phenotypic variability and community interactions of germinating Streptomyces spores
Authors: Xu Ye , Vetsigian Kalin
Published: March 2017

A case can be made for stochastic germination and interactions among germinating spores as beneficial germination strategies in uncertain environments. However, there is little data on how widespread, species-specific or diverse such phenomena are. Focusing on Streptomycetes, a platform was developed for quantification of germination and early growth within communities of spores. We found that the germination process is stochastic at three levels: spores vary in their germination times, mycelium networks grow at different rates, and a fraction of germlings stall their growth shortly after germination. Furthermore, by monitoring how these stochastic properties are affected by spore density and chemicals released from spores, germination interactions were quantified for four species. Stochastically germinating spores were frequently promoted or inhibited by compounds released by spores from the same or different species, and all species had distinct interaction profiles. The spatial distribution patterns were important with clusters of spores behaving differently than individual spores. Aged spores exhibited higher dormancy but could efficiently geminate in the presence of chemicals released during germination. All interactions were specific to germination and only weakly affected growth rates. This work suggests that stochastic germination is commonly affected by the community context and species have adapted diverse germination strategies.
Avian and human influenza virus compatible sialic acid receptors in little brown bats
Authors: Chothe Shubhada K. , Bhushan Gitanjali , Nissly Ruth H. , Yeh Yin-Ting , Brown Justin , Turner Gregory , Fisher Jenny , Sewall Brent J. , Reeder DeeAnn M. , Terrones Mauricio , Jayarao Bhushan M. , Kuchipudi Suresh V.
Published: March 2017

Influenza A viruses (IAVs) continue to threaten animal and human health globally. Bats are asymptomatic reservoirs for many zoonotic viruses. Recent reports of two novel IAVs in fruit bats and serological evidence of avian influenza virus (AIV) H9 infection in frugivorous bats raise questions about the role of bats in IAV epidemiology. IAVs bind to sialic acid (SA) receptors on host cells, and it is widely believed that hosts expressing both SA α2,3-Gal and SA α2,6-Gal receptors could facilitate genetic reassortment of avian and human IAVs. We found abundant co-expression of both avian (SA α2,3-Gal) and human (SA α2,6-Gal) type SA receptors in little brown bats (LBBs) that were compatible with avian and human IAV binding. This first ever study of IAV receptors in a bat species suggest that LBBs, a widely-distributed bat species in North America, could potentially be co-infected with avian and human IAVs, facilitating the emergence of zoonotic strains.
Adaptive immune responses to booster vaccination against yellow fever virus are much reduced compared to those after primary vaccination
Authors: Kongsgaard Michael , Bassi Maria R. , Rasmussen Michael , Skjødt Karsten , Thybo Søren , Gabriel Mette , Hansen Morten Bagge , Christensen Jan Pravsgaard , Thomsen Allan Randrup , Buus Soren , Stryhn Anette
Published: March 2017

Outbreaks of Yellow Fever occur regularly in endemic areas of Africa and South America frequently leading to mass vaccination campaigns straining the availability of the attenuated Yellow Fever vaccine, YF-17D. The WHO has recently decided to discontinue regular booster-vaccinations since a single vaccination is deemed to confer life-long immune protection. Here, we have examined humoral (neutralizing antibody) and cellular (CD8 and CD4 T cell) immune responses in primary and booster vaccinees (the latter spanning 8 to 36 years after primary vaccination). After primary vaccination, we observed strong cellular immune responses with T cell activation peaking ≈2 weeks and subsiding to background levels ≈ 4 weeks post-vaccination. The number of antigen-specific CD8+ T cells declined over the following years. In >90% of vaccinees, in vitro expandable T cells could still be detected >10 years post-vaccination. Although most vaccinees responded to a booster vaccination, both the humoral and cellular immune responses observed following booster vaccination were strikingly reduced compared to primary responses. This suggests that pre-existing immunity efficiently controls booster inoculums of YF-17D. In a situation with epidemic outbreaks, one could argue that a more efficient use of a limited supply of the vaccine would be to focus on primary vaccinations.
From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
Authors: Ceusters J. , Lejeune J.-Ph. , Sandersen C. , Niesten A. , Lagneaux L. , Serteyn D.
Published: March 2017

Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
Experimental and numerical evaluations on palm microwave heating for Red Palm Weevil pest control
Authors: Massa Rita , Panariello Gaetano , Pinchera Daniele , Schettino Fulvio , Caprio Emilio , Griffo Raffaele , Migliore Marco Donald
Published: March 2017

The invasive Red Palm Weevil is the major pest of palms. Several control methods have been applied, however concern is raised regarding the treatments that can cause significant environmental pollution. In this context the use of microwaves is particularly attractive. Microwave heating applications are increasingly proposed in the management of a wide range of agricultural and wood pests, exploiting the thermal death induced in the insects that have a thermal tolerance lower than that of the host matrices. This paper describes research aiming to combat the Red Palm pest using microwave heating systems. An electromagnetic-thermal model was developed to better control the temperature profile inside the palm tissues. In this process both electromagnetic and thermal parameters are involved, the latter being particularly critical depending on plant physiology. Their evaluation was carried out by fitting experimental data and the thermal model with few free parameters. The results obtained by the simplified model well match with both that of a commercial software 3D model and measurements on treated Phoenix canariensis palms with a ring microwave applicator. This work confirms that microwave heating is a promising, eco-compatible solution to fight the spread of weevil.
Implementation of a quantum controlled-SWAP gate with photonic circuits
Authors: Ono Takafumi , Okamoto Ryo , Tanida Masato , Hofmann Holger F. , Takeuchi Shigeki
Published: March 2017

Quantum information science addresses how the processing and transmission of information are affected by uniquely quantum mechanical phenomena. Combination of two-qubit gates has been used to realize quantum circuits, however, scalability is becoming a critical problem. The use of three-qubit gates may simplify the structure of quantum circuits dramatically. Among them, the controlled-SWAP (Fredkin) gates are essential since they can be directly applied to important protocols, e.g., error correction, fingerprinting, and optimal cloning. Here we report a realization of the Fredkin gate for photonic qubits. We achieve a fidelity of 0.85 in the computational basis and an output state fidelity of 0.81 for a 3-photon Greenberger-Horne-Zeilinger state. The estimated process fidelity of 0.77 indicates that our Fredkin gate can be applied to various quantum tasks.
Individual Differences in Moral Disgust Do Not Predict Utilitarian Judgments, Sexual and Pathogen Disgust Do
Authors: Laakasuo Michael , Sundvall Jukka , Drosinou Marianna
Published: March 2017

The role of emotional disgust and disgust sensitivity in moral judgment and decision-making has been debated intensively for over 20 years. Until very recently, there were two main evolutionary narratives for this rather puzzling association. One of the models suggest that it was developed through some form of group selection mechanism, where the internal norms of the groups were acting as pathogen safety mechanisms. Another model suggested that these mechanisms were developed through hygiene norms, which were piggybacking on pathogen disgust mechanisms. In this study we present another alternative, namely that this mechanism might have evolved through sexual disgust sensitivity. We note that though the role of disgust in moral judgment has been questioned recently, few studies have taken disgust sensitivity to account. We present data from a large sample (N = 1300) where we analyzed the associations between The Three Domain Disgust Scale and the most commonly used 12 moral dilemmas measuring utilitarian/deontological preferences with Structural Equation Modeling. Our results indicate that of the three domains of disgust, only sexual disgust is associated with more deontological moral preferences. We also found that pathogen disgust was associated with more utilitarian preferences. Implications of the findings are discussed.
Unacylated ghrelin promotes adipogenesis in rodent bone marrow via ghrelin O-acyl transferase and GHS-R1a activity: evidence for target cell-induced acylation
Authors: Hopkins Anna L. , Nelson Timothy A. S. , Guschina Irina A. , Parsons Lydia C. , Lewis Charlotte L. , Brown Richard C. , Christian Helen C. , Davies Jeffrey S. , Wells Timothy
Published: March 2017

Despite being unable to activate the cognate ghrelin receptor (GHS-R), unacylated ghrelin (UAG) possesses a unique activity spectrum that includes promoting bone marrow adipogenesis. Since a receptor mediating this action has not been identified, we re-appraised the potential interaction of UAG with GHS-R in the regulation of bone marrow adiposity. Surprisingly, the adipogenic effects of intra-bone marrow (ibm)-infused acylated ghrelin (AG) and UAG were abolished in male GHS-R-null mice. Gas chromatography showed that isolated tibial marrow adipocytes contain the medium-chain fatty acids utilised in the acylation of UAG, including octanoic acid. Additionally, immunohistochemistry and immunogold electron microscopy revealed that tibial marrow adipocytes show prominent expression of the UAG-activating enzyme ghrelin O-acyl transferase (GOAT), which is located in the membranes of lipid trafficking vesicles and in the plasma membrane. Finally, the adipogenic effect of ibm-infused UAG was completely abolished in GOAT-KO mice. Thus, the adipogenic action of exogenous UAG in tibial marrow is dependent upon acylation by GOAT and activation of GHS-R. This suggests that UAG is subject to target cell-mediated activation – a novel mechanism for manipulating hormone activity.
The combined effects of sampling parameters on the sorbent tube sampling of phthalates in air
Authors: Jo Sang-Hee , Kim Ki-Hyun , Kwon Kyenghee
Published: March 2017

The adsorption properties of various sorbent materials were investigated to assess the factors affecting biases in the sorbent tube (ST) sampling of airborne phthalates. The recovery of phthalates was assessed critically in relation to four key sampling parameters: (1) three types of sorbent materials (quartz wool (QW), glass wool (GW), and quartz wool plus Tenax TA (QWTN)), (2) the concentration level of phthalate standards, (3) purge flow rate, and (4) purge volume for analysis based on a ‘sorbent tube-thermal desorption-gas chromatography-mass spectrometry (ST-TD-GC-MS)’ system. Among these parameters, the type of ST was the most influential in determining the recovery of phthalates. For a given ST type, the recovery of phthalates tends to improve with increases in the concentration level of standards. In case of QW and QWTN tubes, the breakthrough of phthalates was not observed up to the maximum purge volume (100 L) tested in this work; however, in case of GW, the recovery decreased drastically to 60% even at a purge volume of 1 L for low molecular weight phthalates. The results of our study demonstrate that accurate analysis of airborne phthalates can be achieved through proper control of key sampling parameters, particularly the choice of sorbent material.
Ash aggregation enhanced by deposition and redistribution of salt on the surface of volcanic ash in eruption plumes
Authors: Mueller Sebastian B. , Ayris Paul M. , Wadsworth Fabian B. , Kueppers Ulrich , Casas Ana S. , Delmelle Pierre , Taddeucci Jacopo , Jacob Michael , Dingwell Donald B.
Published: March 2017

Interactions with volcanic gases in eruption plumes produce soluble salt deposits on the surface of volcanic ash. While it has been postulated that saturation-driven precipitation of salts following the dissolution of ash surfaces by condensed acidic liquids is a primary mechanism of salt formation during an eruption, it is only recently that this mechanism has been subjected to detailed study. Here we spray water and HCl droplets into a suspension of salt-doped synthetic glass or volcanic ash particles, and produce aggregates. Deposition of acidic liquid droplets on ash particles promotes dissolution of existing salts and leaches cations from the underlying material surface. The flow of liquid, due to capillary forces, will be directed to particle-particle contact points where subsequent precipitation of salts will cement the aggregate. Our data suggest that volcanically-relevant loads of surface salts can be produced by acid condensation in eruptive settings. Several minor and trace elements mobilised by surface dissolution are biologically relevant; geographic areas with aggregation-mediated ash fallout could be “hotspots” for the post-deposition release of these elements. The role of liquids in re-distributing surface salts and cementing ash aggregates also offers further insight into the mechanisms which preserve well-structured aggregates in some ash deposits.
Sequence analysis of pooled bacterial samples enables identification of strain variation in group A streptococcus
Authors: Weldatsadik Rigbe G. , Wang Jingwen , Puhakainen Kai , Jiao Hong , Jalava Jari , Räisänen Kati , Datta Neeta , Skoog Tiina , Vuopio Jaana , Jokiranta T. Sakari , Kere Juha
Published: March 2017

Knowledge of the genomic variation among different strains of a pathogenic microbial species can help in selecting optimal candidates for diagnostic assays and vaccine development. Pooled sequencing (Pool-seq) is a cost effective approach for population level genetic studies that require large numbers of samples such as various strains of a microbe. To test the use of Pool-seq in identifying variation, we pooled DNA of 100 Streptococcus pyogenes strains of different emm types in two pools, each containing 50 strains. We used four variant calling tools (Freebayes, UnifiedGenotyper, SNVer, and SAMtools) and one emm1 strain, SF370, as a reference genome. In total 63719 SNPs and 164 INDELs were identified in the two pools concordantly by at least two of the tools. Majority of the variants (93.4%) from six individually sequenced strains used in the pools could be identified from the two pools and 72.3% and 97.4% of the variants in the pools could be mined from the analysis of the 44 complete Str. pyogenes genomes and 3407 sequence runs deposited in the European Nucleotide Archive respectively. We conclude that DNA sequencing of pooled samples of large numbers of bacterial strains is a robust, rapid and cost-efficient way to discover sequence variation.
Synthesis of Sr2Si5N8:Ce3+ phosphors for white LEDs via efficient chemical vapor deposition
Authors: Yang Che-Yuan , Som Sudipta , Das Subrata , Lu Chung-Hsin
Published: March 2017

Novel chemical vapor deposition (CVD) process was successfully developed for the growth of Sr2Si5N8:Ce3+ phosphors with elevated luminescent properties. Metallic strontium was used as a vapor source for producing Sr3N2 vapor to react with Si3N4 powder via a homogeneous gas-solid reaction. The phosphors prepared via the CVD process showed high crystallinity, homogeneous particle size ranging from 8 to 10 μm, and high luminescence properties. In contrast, the phosphors prepared via the conventional solid-state reaction process exhibited relative low crystallinity, non-uniform particle size in the range of 0.5–5 μm and relatively lower luminescent properties than the phosphors synthesized via the CVD process. Upon the blue light excitation, Sr2−xCexSi5N8 phosphors exhibited a broad yellow band. A red shift of the emission band from 535 to 556 nm was observed with the increment in the doping amount of Ce3+ ions from x = 0.02 to x = 0.10. The maximum emission was observed at x = 0.06, and the external and internal quantum efficiencies were calculated to be 51% and 71%, respectively. Furthermore, the CVD derived optimum Sr1.94Ce0.06Si5N8 phosphor exhibited sufficient thermal stability for blue-LEDs and the activation energy was calculated to be 0.33 eV. The results demonstrate a potential synthesis process for nitride phosphors suitable for light emitting diodes.