bluuebunny/crossref_metadata_2025 huggingface dataset:
It Takes Two to Tango; A Review of Oncogenic Virus and Host Microbiome Associated Inflammation on Head and Neck Cancer
Authors: McKeon Mallory G. , Gallant Jean-Nicolas , Kim Young J. , Das Suman R.
Published: June 2022

While the two primary risk factors for head and neck squamous cell carcinoma (HNSCC) are alcohol and tobacco, viruses account for an important and significant upward trend in HNSCC incidence. Human papillomavirus (HPV) is the causative agent for a subset of oropharyngeal squamous cell carcinoma (OPSCC)—a cancer that is impacting a rapidly growing group of typically middle-aged non-smoking white males. While HPV is a ubiquitously present virus (with about 7% of the population having oral HPV infection at any one time), only 1% of those infected develop OPSCC— suggesting that additional cofactors or coinfections may be required. Epstein-Barr virus (EBV) is a similarly ubiquitous virus that is strongly linked to nasopharyngeal carcinoma (NPC). Both of these viruses cause cellular transformation and chronic inflammation. While dysbiosis of the human microbiome has been associated with similar chronic inflammation and the pathogenesis of mucosal diseases (including OPSCC and NPC), a significant knowledge gap remains in understanding the role of bacterial-viral interactions in the initiation, development, and progression of head and neck cancers. In this review, we utilize the known associations of HPV with OPSCC and EBV with NPC to investigate these interactions. We thoroughly review the literature and highlight how perturbations of the pharyngeal microbiome may impact host-microbiome-tumor-viral interactions—leading to tumor growth.
Alcohol Dependence Induces CRF Sensitivity in Female Central Amygdala GABA Synapses
Authors: Rodriguez Larry , Kirson Dean , Wolfe Sarah A. , Patel Reesha R. , Varodayan Florence P. , Snyder Angela E. , Gandhi Pauravi J. , Khom Sophia , Vlkolinksy Roman , Bajo Michal , Roberto Marisa
Published: June 2022

Alcohol use disorder (AUD) is a chronically relapsing disease characterized by loss of control in seeking and consuming alcohol (ethanol) driven by recruitment of brain stress systems. However, AUD differs among the sexes: men are more likely to develop AUD, but women progress from casual to binge drinking and heavy alcohol use more quickly. The central amygdala (CeA) is a hub of stress and anxiety, with corticotropin releasing factor (CRF)-CRF1 receptor and GABAergic signaling dysregulation occurring in alcohol dependent male rodents. However, we recently showed that GABAergic synapses in female rats are less sensitive to the acute effects of ethanol. Here, we used patch clamp electrophysiology to examine the effects of alcohol dependence on the CRF-modulation of rat CeA GABAergic transmission of both sexes. We found that GABAergic synapses of naïve female rats were unresponsive to CRF application compared males, although alcohol dependence induced a similar CRF responsivity in both sexes. In situ hybridization revealed that females had less CeA neurons containing mRNA for the CRF1 receptor (Crhr1) than males, but in dependence, the percentage of Crhr1-expressing neurons in females increased, unlike males. Overall, our data provide evidence for sexually dimorphic CeA CRF system effects on GABAergic synapses in dependence.
Malicious Applications Detection in Android using Machine Learning
Authors: Mugees Asif Muhammad , Asif Sana , Mubarik Iqra , Hussain Rabia
Published: June 2022

A huge number of applications available for Android-based smartphone devices have emerged over the past years. Due to which a huge number of malicious applications has been growing explosively. Many approaches have been proposed to ensure the security and quality of application in the markets. Usually, Machine Learning approaches are utilized in the classification process of malicious application detection. Calculating accurate results of characterizing applications behaviors, or other features, has a direct effect on the results with Machine Learning calculations. Android applications emerge so quickly. The behavior of current applications has gotten progressively malicious. The extraction of malware-infected features from applications is thus become a difficult task. According to our knowledge, a ton of features have been extricated in existing work however no survey has overviewed the features built for identifying malicious applications efficiently. In this paper, we will in general give an extensive review of such sort of work that identifies feature applications by describing various practices of uses with various kinds of features. In this survey we have discussed the following dimensions: extraction and selection of feature methods if any, methods of detection and evaluation performed. In light of our review, we notice the issues of investigating malware-affected features from applications, give the scientific categorization and demonstrate the future headings.
High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms
Authors: Adeogun Adedapo O , Omotayo Ahmed , Babalola Ayodele , Joseph Tosin , Adesalu Oluwakemi , Jimoh Romoke , Oyeniyi Tolulope , Awolola Samson , Ladokun Olusola
Published: June 2022

Background: Insecticide resistance in Anopheles gambiae s.l. is a major challenge for malaria vector control in Nigeria. Both target-site insensitivity and metabolic resistance have been im-plicated in resistance process, with the latter receiving little attention in Nigeria. Therefore, we investigated metabolic enzyme activities in Anopheles gambiae s.l populations resistant to Del-tamethrin and Diethyldichlorotriethylethane (DDT) in South-West Nigeria. Methods: Anopheles larvae were collected from Ibadan, Oyo and Badagry, Lagos. Adults were exposed to Deltamethrin and DDT using WHO method. Cohorts of populations were further exposed to Pyperonil Butoxide (PBO) and Deltamethrin. Insecticide-exposed and unexposed co-horts were examined for metabolic enzyme activities. Results were compared between exposed and unexposed samples ANOVA (P<0.05). Results: Mosquitoes were identified as An. gambiae (89%, Ibadan; 0%, Badagry) and An. coluzzii (11%, Ibadan; 100%, Badagry). The populations showed varied level of resistance to Deltamethrin (26%, Ibadan; 71%, Badagry) and DDT (2%, Ibadan; 44%, Badagry). Mortality to Deltamethrin in-creased from 26% to 64% (Ibadan) and 71% to 84% (Badagry) when populations were pre-exposed to PBO. Biochemical analysis revealed significant high levels (P<0.05) of cytochrome P450 and GST in exposed samples. Conclusions: Cytochrome P450 and GST are involved in Deltamethrin and DDT resistance in Anopheles gambiae s.l populations in South-West Nigeria.
Georeferencing Educational Quality in Colombia based on the Phenomena of Agglomeration and Academic Segregation
Authors: Castro-Aristizabal Geovanny , Esteban Gregorio Giménez , Londoño David Arango , Cediel Esteban Moreno , Castillo Caicedo Maribel
Published: June 2022

This study seeks to measure the degree of agglomeration of educational quality in Colombia, based on the non-socialization of the population that exhibits low educational quality, with the population that exhibits high educational quality, and thus determine how such agglomeration affects the phenomenon of academic segregation. To this end, we perform a spatial analysis of the educational quality in Colombia and of variables that may influence to the phenomenon of educational agglomeration. The level of agglomeration in educational quality in Colombia is demonstrated by the calculation of the Moran’s Index, in which a result of 0.62 was obtained. High educational quality is concentrated in the Andean region, while low educational quality is agglomerated in the periphery of the country, in areas such as the Pacific region. A spatial regression model was carried out to measure the dependence of municipalities on their neighbors, and to determine the main socio-economic factors affecting the phenomenon of educational agglomeration in Colombia, finding that living conditions, unsatisfied basic needs and fiscal transparency have all an impact on the educational quality of the municipalities. It is also found that the number of homicides in the municipalities does not seem to have a significant relationship with education.
NIOSH Risk-Based Model to Resume Field Research and Public Health Service in 2020 During the COVID-19 Pandemic
Authors: Johns Douglas O. , Yeoman Kristin M. , Harney Joshua M. , Howard John , Poplin Gerald S.
Published: June 2022

In the early months of the COVID-19 pandemic, field research and public health service work conducted by the National Institute for Occupational Safety and Health (NIOSH) was put on hold. During this time, NIOSH developed a risk-based model to resume fieldwork, balancing the public health benefit of such fieldwork with the risks of severe acute respiratory syndrome coronavirus 2 exposure and transmission. We describe our experiences with this model, along with the broader public health significance of the methods used to inform risk management decisions. (Am J Public Health. 2022;112(8):1138–1141. https://doi.org/10.2105/AJPH.2022.306882 )
THEORETICAL AND METHODOLOGICAL APPROACHES TO BUSINESS MODEL RESEARCH
Authors: Serhiyevich. Т.
Published: June 2022

The theoretical and methodological principles of the study of business models have been clarified. The subject of the research is the business model of an enterprise. The purpose of the research is to develop the theoretical and methodological foundations of the business model of an enterprise. It is shown that the phenomenon of value is based on the subject-object-subject relation and the value of the object depends on the social significance of the act of consumption. Three approaches to the essence of the business model are identified – system-deterministic approach, where the business model is considered as a system of regulation and development of economic relations within the enterprise; resource-reproduction approach, which is based on the principle of reproduction of the structure of the business model through an effective revenue model and supply model; network approach, whose representatives consider the business model as a network of actors jointly creating use value. Analysis of these approaches and synthesis of results allowed us to develop the author's definition of a business model. It is proposed to understand the business model as a stable, isolated, relatively independent mechanism of reproduction of use value, which regulates the emerging economic relations and ensures the viability of the enterprise. The business model defines unique combinations of labor, financial, material, information, scientific and technical and other resources for the creation and appropriation of use value. The specificity of the use value, in turn, is embodied in the peculiarities of the business model. The economic structure of the business model reflects the mechanism for generating and assigning revenue to the enterprise. The business model has a specific organizational structure that institutionalizes interactions within the enterprise. The business model determines the forms of organizing economic relations with other entities – consumers, business partners, suppliers, the state, including the distribution of economic and social benefits between them.
Frecuencia y factores asociados a caídas en pacientes adultos mayores: comparación entre poblaciones residentes en Lima-Callao y andes peruanos
Authors:   , Bancayan Rojas Gianella Paola , Yataco Camarena Dayana Elena
Published: June 2022

INTRODUCCIÓN: Determinar la frecuencia y factores asociados a trastornos de equilibrio y caídas en pobladores adultos mayores a nivel del mar y comunidades altoandinas peruanas. MATERIALES Y MÉTODOS: Estudio tipo observacional, analítico, transversal y análisis secundario de una base de datos. Se evaluó la frecuencia de trastornos del equilibrio mediante la prueba de alcance funcional y el riesgo de caídas en adultos mayores, adicionalmente se estudiaron los factores asociados. RESULTADOS: La muestra estuvo conformada por 2349 adultos mayores. La frecuencia de trastornos del equilibrio fue de 45.9% (n=1060) y la frecuencia de caídas fue de 60.8% (n=1402). Encontramos que la población de Lima – Callao tiene menos probabilidad de presentar trastorno del equilibrio que la población de los Andes, pese a que la frecuencia de trastornos del equilibrio fue mayor en pacientes de Lima – Callao al hacer una regresión de Poisson con varianza robusta encontramos un Pr 0.88 IC (0.79 – 0.97), lo cual indica que las probabilidades de tener trastornos del equilibrio en los participantes de Lima – Callao es significativamente menor en comparación a la población de los Andes. Además, encontramos que la población de Lima – Callao tenía un Pr 0.77 IC (0.67- 0.89), indicando que la población de Lima - Callao tiene menor probabilidades de presentar caídas en comparación con la población de los Andes. Por otro lado, encontramos asociación estadística entre alcance funcional alterado y depresión, deterioro cognitivo, pobre rendimiento físico, riesgo socio familiar, polifarmacia y consumir alcohol. Asimismo, encontramos que la edad, sexo, diabetes, obesidad, deterioro cognitivo, pobre rendimiento físico, riesgo socio familiar, auto reporte de falta de soporte social, consumir alcohol y tabaco, el uso de los servicios de salud y los trastornos del equilibrio fueron factores asociados significativamente a caídas...
Valor de marca y la decisión de compra de los clientes de la marca Master Belle Lima - Perú 2021
Authors:   , Chirapa Romero Jazmin Cristhel , Montalvan Aratia Alejandra
Published: June 2022

La finalidad de la presente investigación estuvo enfocada en determinar la relación entre el valor de marca y la decisión de compra de los clientes de la marca Master Belle Lima - Perú 2021. La investigación asumió el enfoque cuantitativo, el diseño no experimental- transeccional correlacional. Se tomó como población a los 150 clientes actualizados desde el registro de data en la institución al 2021, la muestra utilizada estuvo conformada por 109 clientes de la marca. Se utilizó el cuestionario como instrumento, obteniéndose su confiabilidad por el constante de fiabilidad VM. (0.718) y DC. (0.863). Los cálculos señalaron un correspondencia directa y elevada entre las variables, con una Rho de Spearman (0.639). De esta manera, en los resultados se pudo evidenciar que el valor de marca se encuentra vinculado de manera convincente con la decisión de compra, es decir que, a mayor percepción sobre un buen desarrollo del valor de marca, mayor será la decisión de adquisición por parte de los usuarios hacia los productos de la marca Master Belle.
Nivel de conocimiento sobre tuberculosis multidrogorresistente en la población general que acude al Centro de Salud Sesquicentenario - Callao, 2021
Authors:   , Velasquez Zapata Gabriela Luciana Mercedes , Berrio Moreano Paula Fernanda
Published: June 2022

Objetivo: Determinar el nivel de conocimiento sobre Tuberculosis multidrogorresistente en la población general que acudió a una consulta en el Centro de Salud Sesquicentenario en el año 2021. Materiales y Métodos: Estudio observacional, descriptivo, prospectivo y transversal. El tamaño de la muestra fue de 380 pobladores obtenidos por el programa Epidad 3.1. El material utilizado fue una encuesta validada en 2009 por un juicio de 7 expertos pertenecientes a la Estrategia Sanitaria Nacional de Prevención y Control de Tuberculosis de diversos distritos de Lima, con un coeficiente k-R = 0.8 demostrando una buena confiabilidad de esta. Resultados: A nivel global, los participantes entrevistados tienen un grado de conocimientos Medio (47.1%), seguido de un grado de conocimientos Bajo (41.1%). Acerca de las dimensiones a estudiar: en Definición los participantes tienen un nivel Medio (53.4%), en Modo de Contagio se obtuvo un nivel Alto (38.2%), en Manifestaciones Clínicas se obtuvo un nivel Medio (45.3%), en Tratamiento se obtuvo un nivel Bajo (82.6%) y en Medidas de Prevención se obtuvo un nivel Medio (69.2%). Conclusión: El 88% de la población entrevistada tienen un nivel de conocimientos de Medio a Bajo sobre Tuberculosis multidrogorresistente a pesar de contar con campañas mensuales ofrecidas por el Centro de Salud.
Using MetaboAnalyst 5.0 Part II: Obtaining functional insights from peak list data
Authors: Pang Zhiqiang , Zhou Guangyan , Ewald Jessica , Chang Le , Hacariz Orcun , Basu Niladri , Xia Jianguo
Published: June 2022

Liquid-chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has become a workhorse in global metabolomics studies with growing applications across biomedical and environmental sciences. However, outstanding bioinformatics challenges in terms of data processing, statistical analysis and functional interpretation remain critical barriers to the wider adoption of this technology. To help the user community overcome these barriers, we have made major updates to the well-established MetaboAnalyst platform (www.metaboanalyst.ca). This protocol is part two of four and provides stepwise instructions on how to use MetaboAnalyst to obtain functional insights from peak list data.
Fuzzy Goal Programming approach for solving linear fractional programming problems with fuzzy numbers
Authors: Maiti Indrani , Mandal Tarni , Pramanik Surapati , das sapan kumar , PRASAD RAJEEV
Published: June 2022

The prediction of a real-life problem like in industrial sector or health sector the outcome is impossible or sometimes it is difficult. Due to high information uncertainty and complicated influencing factors of industrial sector, the traditional data-driven prediction approaches can hardly reflect the real changes in practical situation. Fuzzy programming is a powerful prediction reasoning and risk assessment model for uncertain environment. This article mainly explores and applies a modified form of fuzzy programming; namely Fuzzy Linear Fractional Programming Problem (FLFPP) having the coefficients of the objectives and constraints as triangular fuzzy numbers (TFNs). The FLFPP is converted into an equivalent crisp multi-objective linear fractional programming problem (MOLFPP) and solved individually to associate an aspiration level to it. Then by applying fuzzy goal programming (FGP) technique the maximum degree of each membership goal is obtained by minimizing the negative deviational variables. We carry out two industrial application simulations in a hypothetical industrial scenario. Our study shows that the proposed model is practical and applicable to the uncertain practical environment to realize the prediction and the obtained results are compared with that of the existing methods.
Improving the management of chronic pain, opioid use, and opioid use disorder in older adults: study protocol for I-COPE study
Authors: Kagarmanova Ainur , Sparkman Heather , Laiteerapong Neda , Thompson Katherine , Rosul Linda , Lazar Danielle , Staab Erin , Wan Wen , Kass Amanda , Ari Mim
Published: June 2022

Background.
Older adults with chronic pain, opioid use, and opioid use disorder (OUD) present complex management decisions in primary care. Clinical tools are needed to improve care delivery. This study protocol describes the planned implementation and evaluation of I-COPE (Improving Chicago Older Adult Opioid and Pain Management through Patient-centered Clinical Decision Support and Project ECHO®) to improve care for this population.
Methods.
This study uses a pragmatic, expanding cohort stepped-wedge design to assess outcomes. The study will be implemented in 35 clinical sites across metropolitan Chicago for patients aged ≥ 65 with chronic pain, opioid use, or OUD who receive primary care at one of the clinics. I-COPE includes the integration of patient-reported data on symptoms and preferences, clinical decision support tools, and a shared decision-making tool into routine primary care for more effective management of chronic pain, opioid prescribing, and OUD in older adults. Primary care providers will be trained through web-based videos and an optional Project ECHO® course, entitled “Pain Management and OUD in Older Adults.” The RE-AIM framework will be used to assess the I-COPE implementation. Effectiveness outcomes will include increased variety of recommended pain treatments, decreased prescriptions of higher-risk pain treatments, and decreased patient pain scores. All outcomes will be evaluated 6 and 12 months after implementation. PCPs participating in Project ECHO® will be evaluated on changes in knowledge, attitudes, and self-efficacy using pre- and post-course surveys.
Discussion.
This study will provide evidence about the effectiveness of collecting patient-reported data on symptoms and treatment preferences and providing clinical decision support and shared decision-making tools to improve management for older adults with chronic pain, opioid use, and OUD.
Trial registration
: The study is registered at ClinicalTrials.gov under #NCT04878562.
Osseous healing of the CORA-based leveling osteotomy stabilized with a bone plate, cannulated compression screw, and tension band: a descriptive case series in dogs
Authors: Johnson Tiffany A. , Krier Elizabeth M. , Hulse Don A. , Beale Brian S. , Hudson Caleb C. , Old Gary T. , Peycke Laura E.
Published: June 2022

Background
An osteotomy procedure option for stabilization of the CrCL deficient stifle is the Center of Rotation of Angulation (CORA) Based Leveling Osteotomy (CBLO). The CBLO procedure involves a proximal tibial plateau leveling osteotomy based on the magnitude of the CORA. Correction of the CORA magnitude results in the desired postoperative tibial plateau angle (TPA) without secondary translation. If translation is prevented, the amount of bone contact across the osteotomy is maximized. This coupled with the ability to apply compression across the osteotomy with a cannulated compression screw (CCS) and tension band increases the strength of the construct. The objectives of this descriptive case series is to assess osseous bridging of the osteotomy at 35 days following a CBLO stabilized with a bone plate, CCS, and tension band. A second objective is to determine if there is a shift of the postoperative TPA when compared to TPA at final radiographic evaluation.
Results
Forty-seven dogs (49 stifles) met the inclusion criteria. Multiple breeds were represented with a mean age of 6 years and mean body mass of 32kg. All dogs had incompetent partial or complete CrCL tears. There was approximately 50% incidence of medial meniscal pathology with remaining intra-articular structures appearing normal. No intraoperative complications occurred; there were two postoperative complications including a late onset medial meniscal tear and an implant removal due to infection. At a mean time of 35 days (range 25–39 days) postoperatively, osseous bridging of the osteotomy was Grade 4 in 38 stifles (78%) and Grade 3 in 11 stifles (22%). There was no difference between postoperative TPA and final TPA.
Conclusions
Stabilization of a CBLO osteotomy with a bone plate, CSS, and tension band resulted in the majority of dogs (78%) exhibiting Grade 4 healing and the remainder of dogs (22%) showing Grade 3 healing of the osteotomy at a median of 35 days (range 25days to 39 days) postoperatively. Further, there was no TPA shift recognized at final radiographic assessment.
The clinical characteristics of children with agenesis of the corpus callosum
Authors: duan yuanhui , cao jie
Published: June 2022

Objective: To analyze the clinical features of children with agenesis of the corpus callosum in this study due to the lack description at present. Method: This study retrospectively collected the case data of the agenesis of corpus callosum (ACC) hospitalized in Children's Hospital Affiliated to Chongqing Medical University from January 2015 to October 2021. Results :1.Classification and typing characteristics: 1.1 Classification according to the absence of anatomical structure of corpus callosum: total absence of corpus callosum in 7 cases (5.8%); partial absent of corpus callosum in 80 cases (66.7%) ;Corpus callosum was thin in 33 cases (27.5%). 1.2 Classification according to presence of extra-callosal craniocerebral malformations:69 children with isolated ACC (57.5%); complex ACC with 51 cases (42.5%). 1.3 Classification based on extracranial structural malformations: 52 children (43.3%) with non-syndromic ACC. 68 cases (56.7%) with syndromic ACC. 2. Clinical features: 70 cases (58.3%) of Gross Developmental Delay, 50 cases (41.7%) of epilepsy, 44 cases (37.3%) of microcephaly, 41 cases (34.2%) of malnutrition, 20 cases (16.7%) of respiratory failure. Conclusion: Children with agenesis of corpus callosum are often accompanied by abnormalities of other intracranial structures outside the corpus callosum and abnormal structure of extracranial organs. Children with agenesis of the corpus callosum often have neurological diseases, such as gross developmental delay, epilepsy. Secondly, the incidence of malnutrition is also high, especially the severe malnutrition. In addition, some children may also show respiratory failure, so it is necessary to be alert to the possibility of structural abnormalities of the respiratory system. The children with agenesis of the corpus callosum also have a family history of spontaneous abortion in their mothers, patients with neuropsychiatric diseases in their families, and the family history of early death.
Implementation components and factors that influence the provision of tele-audiology services for children and adults - a mixed-methods approach
Authors: Ramkumar Vidya , Shankar Varsha , Kumar Shuba
Published: June 2022

Background
Tele-audiology has accelerated with the rapid advancement in information and communication technology. There is a significant knowledge gap concerning the use of implementation science and factors influencing tele-audiology as a routine or long-term, sustained effort. This mixed-methods study aimed to identify implementation factors that influence the provision of tele-audiology services.
Method
We carried out a mixed-methods study comprising a scoping review and semi-structured interviews (SSI). Articles describing tele-audiology were included based on a set of inclusion and exclusion criteria.
Results
Data was extracted from 32 studies that were mapped to 21 projects in tele-audiology. The broad areas of focus identified in tele-audiology included the provision of hearing screening, diagnostic evaluation, a combination of diagnostic audiological with otorhinolaryngological evaluation, hearing aid fitting and programming, cochlear implant fitting and mapping, aural re/habilitation services and comprehensive audiological services. Provision of diagnostic and therapeutic services were predominantly carried out using synchronous/ real-time telepractice methods. Asynchronous methods were used predominantly for audiometric screening, video-otoscopy and tympanometry. The most commonly used model was the ‘professional-facilitator-patient’ followed by the ‘professional-patient’ model. Barriers to long-term sustainability included insufficient initial financial investment, lack of reimbursement policies, limited internet (speed and bandwidth), and lack of organisational policies and uniform laws. Successful implementation was reported to be aided by having a stable source of funding, a devoted team of professionals and technicians with defined roles and duties, and with methodical planning.
Conclusion
The scoping review, as well as the interviewees, represented tele-audiology implementation efforts in a wide range of countries that include low-middle income and high-income nations. Successful implementation was reported to be aided by having a stable source of funding, a devoted team of professionals and technicians with defined roles and duties, and with methodical planning. Sustained implementation occurred when the need and usefulness of tele-audiology were accepted by public sector policy-makers. However, a more accurate understanding of sustainability can be obtained by the use of implementation science and frameworks to guide tele-audiology services.
A green plasmonic nanosensor based on AuNPs for bacteria detection in urine
Authors: Ángeles-Pascual A. , Breton-Deval L.
Published: June 2022

Today the health problems related to infection by bacteria and virus have increased notably worldwide; for this reason, the demand for reliable and rapid detection methods are necessary. In this work we present a study of colorimetric bacteria in the urine based on 4-mercaptophenylboronic acid (4-MPBA) functionalized with gold nanoparticles (AuNPs) as a green plasmonic nanosensor (GPN) synthesized via novel and simple hand-made Cinnamomum verum extract by green chemistry. Three molar concentrations of AuNPs were synthesized (1, 2 and 3 mM). The structural characterization results by X-ray diffraction (XRD) indicated the presence of AuNPs, chemical and morphological analysis by high-resolution field emission scanning electron microscopy (HR-FESEM) technique indicated the presence of AuNPs and size distribution of 15, 37 and 135.5 nm, respectability, being 1 mM the concentration the one that produces AuNPs with size control and good dispersion and used to determine the bacterial quantification at concentrations of N1 = 60 mg, N2 = 105 mg and N3 = 120 mg of AuNPs. The urine concentrations were analyzed in the absorbed light in the UV region at different bacteria concentrations, been the concentrations of 120 mg that showed best linear relationship between the absorbance at 534 nm and was the ideal to achieve an equilibrium between the free bacteria and the nanoparticles.
Effectiveness of the Unified Barlow Protocol (UP) and neuropsychological treatment in cancer survivors for cognitive impairments: study protocol for a randomized controlled trial
Authors: Garcia-Torres Francisco , Tejero-Perea Adrián , Gómez-Solís Ángel , Castillo-Mayén Rosario , José Jaén-Moreno Maria , Luque-Salas Bárbara , Gálvez-Lara Mario , Sánchez-Raya Araceli , Jablonski Marcin , Rodríguez-Alonso Beatriz , Aranda Enrique
Published: June 2022

Background: Cancer survivors frequently develop cognitive impairment, which negatively affects their quality of life and emotional well-being. This study compares the effectiveness of a well-established treatment (neuropsychological treatment) with the Unified Protocol for Transdiagnostic Treatment of Emotional Disorders (UP) to reduce these cognitive deficits and evaluate the effect of both treatments on anxiety-depressive symptoms and the quality of life of cancer survivors.Methods: A three-arm, randomized superiority clinical trial with a pre-post and repeated follow-up measures intergroup design using a 1:1:1 allocation ratio will be performed. One hundred and twenty-three cancer survivors with mild to moderate cognitive impairment will be randomly assigned to one of the study interventions: a cognitive rehabilitation intervention group, an intervention group with UP intervention, or a control group on the waiting list. The primary outcome is to observe a significant improvement in cognitive function in both intervention groups and a significant decrease in emotional impairments in comparison with the waitlist group. Improvements in anxiety, depression and quality of life are also expected as secondary outcomes. These results will be maintained at six months of follow-up.Discussion: The aim of this trial is to test the efficacy of the UP intervention in reducing cognitive deficits in breast cancer survivors. The results of this trial may be useful in reducing the presence of cognitive problems in cancer survivors and improving their emotional state and quality of life.Clinical Trial registration: ClinicalTrials.gov ID: NCT05289258, Registered 12 March 2022, v01.
The potential global distribution of the invasive alien species Melinis minutiflora under current and future climates
Authors: Maciel Josiane Costa , Silva Ricardo Siqueira , Duque Tayna Sousa , Santos Marinalva Martins , Silva Daniel Valadão , Fernandes Bruno Caio Chaves , Chagas Paulo Sérgio Fernandes das , Santos José Barbosa
Published: June 2022

The invasive exotic plant Melinis minutiflora P.Beauv. is a globally invasive species and impacts the economy, biodiversity, and human well-being. The challenges of biological invasion may be intensified by climate change. The increase in temperatures and concentrations of greenhouse gases may favor the introduction, establishment, and expansion potential of this species. Climate change may influence the effective management of invasive species. The objectives of this study were, for the first time, to use CLIMEX software to determine the distribution potential of M. minutiflora in the world under current climatic conditions and global climate change scenarios for the 2030, 2050, 2070 and 2100. Modeling with CLIMEX was used to research the impacts of climate change on the potential global distribution of M. minutiflora. The potential worldwide distribution of M. minutiflora under current climatic conditions is broad, with suitable climatic conditions expanding in tropical and subtropical regions. Future climate scenarios of 2030, 2050, 2070, and 2100 M. minutiflora will grow poleward and upward as the climate warms. Where climate change results in expanding the species global potential range, strategies must be analyzed so that strategic control plans for biological invasion are often considered. Understanding this threat can help formulate effective prevention, introduction, and response measures in regions deemed inadequate.
Electrodiagnosis of Guillain–Barré Syndrome Reflecting Acute Inflammatory Demyelinating Polyneuropathy with Reversible Conduction Failure: A Concept of Nodopathy
Authors: Kim Sooyoung , Lee Eun Kyoung , Sohn Eunhee
Published: June 2022

Reversible conduction failure (RCF) has been documented in acute motor axonal neuropathy (AMAN) and is considered a sign of nodopathy. Several reports of RCF in acute inflammatory demyelinating polyneuropathy (AIDP) have suggested that it could be a manifestation of nodopathy. We conducted this study to determine the frequency of RCF in AMAN and AIDP and to compare the clinical features between the two groups with or without RCF. RCF was observed in 38.9% and 18.5% patients in the AMAN and AIDP groups in our study, respectively. AIDP patients with anti-ganglioside antibodies represented 29.4% of the cohort. The clinical features of AIDP with RCF were more similar to those of AMAN with RCF than to those of typical AIDP. However, there were no significant differences in the frequency of anti-ganglioside antibody status between the groups. AIDP with RCF may be a manifestation of nodopathy. The current dichotomous electrodiagnostic criteria, classifying demyelinating and axonal neuropathy, are insufficient to define nodopathy. Further studies are required to revise the electrodiagnostic criteria for Guillain–Barré syndrome.
Design and Synthesis of Magnetic Immobilized Pineapple Cellulose/EDTA for Removal of Copper(II) and Zinc(II) by Coordination
Authors: Sayahi Hanan , Asadabadi Simin
Published: June 2022

In recent decades, treatment of polluted water has shifted towards the enhancement of environmentally friendly adsorbents. In this regard, a biosorbent originated from pineapple peel cellulose was synthesized and used for the adsorption of Cu2+ and Zn2+. The magnetic (3-aminopropyl)triethoxysilane was designated to immobilize modified cellulose and ethylenediaminetetraacetic acid. The biosorbent was characterized by FE-SEM, TEM, EDX, XRD, FT-IR, BET, VSM and TGA techniques. The influence of pH, temperature and biosorbent dosage on the cation removal was investigated by Central Composite Design. The maximum uptake% was 93.18 and 96.30% for Cu2+ and Zn2+, respectively. Coordination and electrostatic attraction were proposed as the main reasons for the removal of heavy metal cations. The adsorption of Zn2+ was ultrafast and completed within 40 s and it took 420 s for totally removal of Cu2+. External surface biosorption, the intra-particle diffusion and the diffusion through the smaller pores and slits, in sequence, were the dominant steps in the adsorption of each cation. Modified pseudo-n-order and modified Langmuir-Freundlich were the best models to describe kinetics and equilibrium results. The maximum adsorption capacity of biosorbent for Zn2+ was higher than one for Cu2+ (153.9 versus 149.4 mg·g-1). The regenerating of biosorbent was successfully accomplished for five cycles using a solution of EDTA in the presence of ultrasonic waves.
Sex, menopause, and Alzheimer’s risk: a 31P-MR spectroscopy study of brain mitochondrial function in association with 11C-PiB PET amyloid-beta load
Authors: Jett Steven , Dyke Jonathan , Andy Caroline , Schelbaum Eva , Jang Grace , Yepez Camila Boneu , Pahlajani Silky , Díaz Iván , Brinton Roberta Diaz , Mosconi Lisa
Published: June 2022

Increasing evidence implicates sex and endocrine aging effects on brain bioenergetic aging in the greater lifetime risk of Alzheimer’s disease (AD) in women. We conducted 31Phosphorus Magnetic Resonance Spectroscopy (31P-MRS) to assess the impact of sex and menopause on brain high-energy phosphates [adenosine triphosphate (ATP), phosphocreatine (PCr), inorganic phosphate (Pi)] and membrane phospholipids [phosphomonoesters/phosphodiesters (PME/PDE)] in 216 midlife cognitively normal individuals at risk for AD, 80% female. Ninety-seven participants completed amyloid-beta (Aβ) 11C-PiB PET. Women exhibited higher ATP utilization than men in AD-vulnerable frontal, posterior cingulate, fusiform, medial and lateral temporal regions (p < 0.001). This profile was evident in frontal cortex at the pre-menopausal and peri-menopausal stage and extended to the other regions at the post-menopausal stage (p = 0.001). Results were significant after multi-variable adjustment for age, APOE-4 status, midlife health indicators, history of hysterectomy/oophorectomy, use of menopause hormonal therapy, and total intracranial volume. While associations between ATP/PCr and Aβ load were not significant, individuals with the highest Aβ load were post-menopausal and peri-menopausal women with ATP/PCr ratios in the higher end of the distribution. No differences in Pi/PCr, Pi/ATP or PME/PDE were detected. Outcomes are consistent with dynamic bioenergetic brain adaptations that are associated with female sex and endocrine aging.
A Graph Theory-based Classification of Mild Cognitive Impairment Using Functional Connectivity Analysis on EEG
Authors: Echeverri-Ocampo Isabel , Ardila-López Karen , Molina-Mateo José , Padilla-Buriticá Jorge Ivan , Llamur Ismael , Restrepo Francia , Segura-Giraldo Belarmino , Iglesia-Vaya Maria
Published: June 2022

Understanding how global brain networks are affected in mild cognitive impairment may explain the changes concerning the electrophysiology of the brain. We research functional changes within neuronal networks in frontal, temporal, parietal lobe and central zone using graph theory in visual oddball paradigm. Thirty subjects (19 females; mean age of 70.63 years old, age range 61 to 79 years old, 11 males; mean age of 70.36 years old, and age range 63 to 81 years old) 14 with mild cognitive impairment and 16 health control. Oddball protocol with target and no target stimuli electroencephalography (EEG) signal were analyzed through functional connectivity (FC) along with Change point detection method and a detailed preprocessing in order to detect dynamical changes in the signal, which reflect the non-stationary behavior of a given EEG signal. Linear Discriminant Analysis (LDA), K-Nearest Neighbor (KNN) and Decision Tree (DT) were trained to classify brain activity in Mild Cognitive Impairment (MCI) and Health Control (HC) subjects based on graph measurements and the physiology interpretation of the Mild Cognitive Impairment. The findings show that using a combination of EEG-based model-free functional connectivity measurements and machine learning to categorize cognitive impairment is effective. The research can be expanded upon to study the possibilities of identifying cognitive decline in real-time, dynamic, and complicated real-world circumstances.
Reaction of nitrocefin with two serines of Pseudomonas aeruginosa penicillin-binding-protein 3
Authors: Newman Hector , Bellini Dom , Krajnc Alen , Tumber Anthony , Tod Julie , Lloyd Adrian , Schofield Christopher , Dowson Christopher
Published: June 2022

Despite their clinical importance, the mechanisms by which β-lactams inhibit penicillin-binding-proteins (PBPs) are not fully defined. X-ray crystallographic and mass spectrometric data studies of a Pseudomonas aeruginosa PBP3 active site variant (Tyr409Ala) reveal evidence for the in vitro acylation of P. aeruginosa PBP3 by reaction of nitrocefin with both Ser294 and Ser349. The results reveal a potentially novel binding mode for β-lactams with PBPs and will inform the design of new PBP inhibitors.
Clinical, Immunological and Molecular Findings of Patients with Severe Combined Immunodeficiency: Introducing of 7 Novel Mutations in 7 Genes
Authors: Alizadeh Zahra , Fazlollahi Mohammad Reza , Mazinani Marzieh , Badalzadeh Mohsen , Heydarlou Hanieh , Carapito Raphael , Molitor Anne , de Oteyza Andrés Caballero Garcia , Proietti Michele , bavani Maryam Soleimani , Shariat Mansoureh , Fallahpour Morteza , Movahedi Masoud , Moradi Leila , Grimbacher Bodo , Bahram Siamak , Pourpak Zahra
Published: June 2022

Purpose
Severe combined immunodeficiency (SCID) is one of the severe inborn errors of the immune system associated with life-threatening infections. This disorder is characterized by diminished cellular and humoral immunity. A variable phenotype, including atypical and leaky SCID cases, as well as the heterogeneity in its genetic basis, may lead to a significant delay in diagnosis. Therefore, early diagnosis of SCID patients is crucial. Here, we describe the clinical manifestations and genetic results of four patients with SCID and four patients with atypical SCID.
Methods
Eight patients, who were clinically suspected to SCID, underwent immunological evaluations and genetic studies. Whole Exome Sequencing (WES) was used to identify the most relevant genetic defects which are responsible for the patients’ clinical phenotype. Further, the identified mutations were evaluated by Sanger sequencing in patients and their parents.
Results
All patients (4 males and 4 females) presented SCID phenotype in early infancy within 6 months of life. We found eight mutations, including two novel mutations in RAG2 (p.I273T, p.G44X), a novel 2-bp deletion in IL7R (p.F361WfsX17), a novel 2-bp deletion in LAT (p.Y207fs), a novel mutation in ADA (c.780 + 1G > A), a novel mutation in JAK3 (p.Q228Ter), a novel mutation in LIG4 (p.G428R), and also a previously reported missense mutation in RAG1 (p.A444V). All patients were homozygous, and their parents were heterozygous.
Conclusion
The results of our study expand the clinical and molecular spectrum associated with SCID and leaky SCID phenotypes and provide valuable information for the clinical management of our patients.
Post-pollination endogenous phytohormone levels in reproductive organs in 2 interspecific Arachis crosses differing in compatibility
Authors: Li Jia Kai , Wang Zhi Wei , Yang Zhen , Song Guo Sheng , Wang Xiu Zhen , Wang Chuan Tang
Published: June 2022

Wild Arachis species are of great value to the genetic improvement of the cultivated peanut; however, incompatibility hinders the utilization of the species outside section Arachis. Endogenous phytohormone imbalance was hypothesized but is yet to be confirmed. Using UPLC-ESI-MS/MS, we were able to determine the phytohormone levels in flower bases 3 days after pollination and pegs/pods 5 and 10 days after soil penetration. The results showed that as far as the significantly changed phytohormones were considered, with the advancement of reproductive development, the proportion of down-regulated auxins and cytokinins gradually increased. Significantly reduced levels of gibberellins in pegs/pods were also recorded. The study suggested that to get desirable results in overcoming incompatible obstacles in wide crosses in Arachis, early post-pollination application of hormone mixtures incorporating auxins, cytokinins and gibberellins should be beneficial.
Sex Differences in Association between Abdominal Obesity and Cardiovascular Death in Patients with Permanent Pacemaker Implantation: a Retrospective Cohort Study
Authors: Xue Xiaodi , Li Xiaoyao , Zhao Shuang , Chen Keping , Hua Wei , Su Yangang , Liang Zhaoguang , Xu Wei , Zhang Shu
Published: June 2022

Objective
This study aimed to explore the associations between abdominal obesity and cardiovascular death (CVD) in patients with permanent pacemakers (PPMs) implantation and to investigate the role of gender on the possible associations. In this regard, this study investigated the association between waist circumference (WC) and cardiovascular death in patients with permanent pacemakers (PPMs) implantation.
Methods
This was a retrospective cohort study that included the data of patients who underwent PPMs implantation in Fuwai Hospital, China from May 2010 to April 2014 using BIOTRONIK Home Monitoring® database. Waist circumference was classified into sex-specific quartiles and patients were divided into three groups according to their body mass index (BMI) values: normal (≤ 22.9 kg/m2), overweight (23–24.9 kg/m2), and obese (≥ b25 kg/m2). Cox proportional hazards models were used to calculate the hazard ratios and 95% confidence intervals for cardiovascular death based on the patient’s WC and BMI.
Results
A total of 492 patients with PPMs implantation were analyzed (mean age: 71.9 ± 10.8 years; 55.1% men [n = 271]). Data showed that after a mean follow-up period of 67.2 ± 17.5 months, 24 (4.9%) patients had experienced cardiovascular death and 71 (14.4%) were cases of all-cause mortality. The men in the third quartile of WC had a hazard ratio (HR) of 10.67 (Model 4, 95% CI: 1.00–115.21, P-trend = 0.04) for cardiovascular death. However, the association disappeared in the women patients (Model 4, HR = 3.99, 95% CI: 0.37–42.87, P-trend = 0.25). No association was found between BMI and cardiovascular death or all-cause mortality in both men and women patients.
Conclusions
Abdominal obesity was associated with an increased risk of cardiovascular death in patients with PPMs and this association is gender dependence where only men patients show this association.