bluuebunny/crossref_metadata_2025 huggingface dataset:
Consequences of Traditional Management in the Production and Quality of Copal Resin (Bursera bipinnata (Moc. & Sessé ex DC.) Engl.) in Mexico
Authors: Abad-Fitz Itzel , Maldonado-Almanza Belinda , Aguilar-Dorantes Karla María , Sánchez-Méndez Luis , Gómez-Caudillo Leopoldo , Casas Alejandro , Blancas José , García-Rodríguez Yolanda Magdalena , Beltrán-Rodríguez Leonardo , Sierra-Huelsz José Antonio , Cristians Sol , Moreno-Calles Ana Isabel , Torres-García Ignacio , Espinosa-García Francisco J.
Published: September 2020

Copal is a resin of ritual uses in Mexico that is extracted from several species of trees of the genus Bursera. The effect of traditional management on phenotypical traits of copal trees has not been sufficiently studied. This research analyzed the traditional management and human selection on populations of Bursera bipinnata, and it also examined their influence on the quantity and quality of the resin produced by wild and managed trees. The management of copal was documented through semi-structured interviews and workshops. Samples of 60 trees from six wild and managed populations were selected to quantify the production of resin during two consecutive years. Fresh resin was collected to identify organic volatile compounds through gas chromatography and Principal Components Analysis (PCA); individuals were classified according to the amount and type of organic compounds produced. We identified management strategies from simple harvesting to seeds planting. The criteria of local people for selecting managed trees and seeds are based on the quantity and quality of the resin produced per tree, which were significantly higher in managed than in wild trees: 190.17 ± 329.04 g vs. 29.55 ± 25.50 g (p = 0.003), and 175.88 ± 179.29 g vs. 63.05 ± 53.25 g (p = 0.008) for the production seasons of 2017 and 2018, respectively. Twenty organic volatile compounds were identified, and the PCA showed that managed trees produce higher percentages of compounds associated with scent. The traditional management of Bursera bipinnata involves selective pressures, which generate the differentiation of wild and managed trees that may represent incipient domestication through silvicultural management.
Monitoring the Processing of Dry Fermented Sausages with a Portable NIRS Device
Authors: González-Mohino Alberto , Pérez-Palacios Trinidad , Antequera Teresa , Ruiz-Carrascal Jorge , Olegario Lary Souza , Grassi Silvia
Published: September 2020

This work studies the ability of a MicroNIR (VIAVI, Santa Rosa, CA) device to monitor the dry fermented sausage process with the use of multivariate data analysis. Thirty sausages were made and subjected to dry fermentation, which was divided into four main stages. Physicochemical (weight lost, pH, moisture content, water activity, color, hardness, and thiobarbiruric reactive substances analysis) and sensory (quantitative descriptive analysis) characterizations of samples on different steps of the ripening process were performed. Near-infrared (NIR) spectra (950–1650 nm) were taken throughout the process at three points of the samples. Physicochemical data were explored by distance to K-Nearest Neighbor (K-NN) cluster analysis, while NIR spectra were studied by partial least square–discriminant analysis; before these models, Principal Component Analysis (PCA) was performed in both databases. The results of multivariate data analysis showed the ability to monitor and classify the different stages of ripening process (mainly the fermentation and drying steps). This study showed that a portable NIR device (MicroNIR) is a nondestructive, simple, noninvasive, fast, and cost-effective tool with the ability to monitor the dry fermented sausage processing and to classify samples as a function of the stage, constituting a feasible decision method for sausages to progress to the following processing stage.
Dynamic Mechanical Analysis as a Complementary Technique for Stickiness Determination in Model Whey Protein Powders
Authors: O’Donoghue Laura , Haque Md. , Hogan Sean , Laffir Fathima , O’Mahony James , Murphy Eoin
Published: September 2020

The α-relaxation temperatures (Tα), derived from the storage and loss moduli using dynamic mechanical analysis (DMA), were compared to methods for stickiness and glass transition determination for a selection of model whey protein concentrate (WPC) powders with varying protein contents. Glass transition temperatures (Tg) were determined using differential scanning calorimetry (DSC), and stickiness behavior was characterized using a fluidization technique. For the lower protein powders (WPC 20 and 35), the mechanical Tα determined from the storage modulus of the DMA (Tα onset) were in good agreement with the fluidization results, whereas for higher protein powders (WPC 50 and 65), the fluidization results compared better to the loss modulus results of the DMA (Tα peak). This study demonstrates that DMA has the potential to be a useful technique to complement stickiness characterization of dairy powders by providing an increased understanding of the mechanisms of stickiness.
In Vitro α-Glucosidase and α-Amylase Inhibitory Activities of Free and Bound Phenolic Extracts from the Bran and Kernel Fractions of Five Sorghum Grain Genotypes
Authors: Xiong Yun , Ng Ken , Zhang Pangzhen , Warner Robyn Dorothy , Shen Shuibao , Tang Hsi-Yang , Liang Zijian , Fang Zhongxiang
Published: September 2020

Diabetes is a global health challenge. Currently, an effective treatment for diabetes is to reduce the postprandial hyperglycaemia by inhibiting the carbohydrate hydrolysing enzymes in the digestive system. In this study, we investigated the in vitro α-glucosidase and α-amylase inhibitory effects of free and bound phenolic extracts, from the bran and kernel fractions of five sorghum grain genotypes. The results showed that the inhibitory effect of sorghum phenolic extracts depended on the phenolic concentration and composition. Sorghum with higher phenolic contents generally had higher inhibitory activity. Among the tested extracts, the brown sorghum (IS131C)-bran-free extract (BR-bran-free, half-maximal inhibitory concentration (IC50) = 18 ± 11 mg sorghum/mL) showed the strongest inhibition against α-glucosidase which was comparable to that of acarbose (IC50 = 1.39 ± 0.23 mg acarbose/mL). The red sorghum (Mr-Buster)-kernel-bound extract (RM-kernel-bound, IC50 = 160 ± 12 mg sorghum/mL) was the most potent in inhibiting α-amylase but was much weaker compared to acarbose (IC50 = 0.50 ± 0.03 mg acarbose/mL).
Insights on Health and Food Applications of Equus asinus (Donkey) Milk Bioactive Proteins and Peptides—An Overview
Authors: Derdak Reda , Sakoui Souraya , Pop Oana Lelia , Muresan Carmen Ioana , Vodnar Dan Cristian , Addoum Boutaina , Vulturar Romana , Chis Adina , Suharoschi Ramona , Soukri Abdelaziz , El Khalfi Bouchra
Published: September 2020

Due to its similarity with human milk and its low allergenic properties, donkey milk has long been used as an alternative for infants and patients with cow’s milk protein allergy (CMPA). In addition, this milk is attracting growing interest in human nutrition because of presumed health benefits. It has antioxidant, antimicrobial, antitumoral, antiproliferative and antidiabetic activity. In addition, it stimulates the immune system, regulates the gastrointestinal flora, and prevents inflammatory diseases. Although all donkey milk components can contribute to functional and nutritional effects, it is generally accepted that the whey protein fraction plays a significant role. This review aims to highlight the active proteins and peptides of donkey milk in comparison with other types of milk, emphasizing their properties and their roles in different fields of health and food applications.
Analyzing Differentially Expressed Genes and Pathways Associated with Pistil Abortion in Japanese Apricot via RNA-Seq
Authors: Shi Ting , Iqbal Shahid , Ayaz Aliya , Bai Yang , Pan Zhenpeng , Ni Xiaopeng , Hayat Faisal , Saqib Bilal Muhammad , Khuram Razzaq Muhammad , Gao Zhihong
Published: September 2020

Reproduction is a critical stage in the flower development process, and its failure causes serious problems affecting fruit quality and yield. Pistil abortion is one of the main factors in unsuccessful reproduction and occurs in many fruit plants. In Japanese apricot, the problem of pistil abortion is very common and affects fruit quality and plant yield; however, its molecular mechanism is not clearly understood. Therefore, in the current study, we used RNA-Seq to identify the differentially expressed genes (DEGs) and pathways actively involved in pistil abortion. A total of 3882 differentially expressed genes were found after cutoff and pairwise comparison analysis. According to KEGG pathway analysis, plant hormone signaling transduction and metabolic pathways were found most significantly enriched in this study. A total of 60 transcription factor families such as MADS-box, NAC and TCP showed their role in this process. RT-qPCR assays confirmed that the expression levels were consistent with RNA-Seq results. This study provides an alternative to be considered for further studies and understanding of pistil abortion processes in Japanese apricot, and it provides a reference related to this issue for other deciduous fruit crops.
Exploring the Dzi Bead with Synchrotron Light: XRD, XRF Imaging and μ-XANES Analysis
Authors: Reinhardt Averie , Feng Renfei , Xiao Qunfeng , Hu Yongfeng , Sham Tsun-Kong
Published: September 2020

The origin of Dzi beads, also called “tian zhu”, has always been a mystery. These beads come in a variety of patterns, shapes and sizes. They have cultural and heritage significance in Tibet and areas surrounding the Himalayas. The most recognized beads are those with the “eye” pattern. They are said to ward off evil spirits. Due to their reputation, the demand for Dzi beads has increased in Asia. Herein, we report a study of a Dzi bead with a three-eye pattern using X-ray diffraction (XRD), X-ray fluorescence (XRF), X-ray absorption near edge structure (XANES) and imaging techniques. This is a novel area for Dzi bead research using X-rays from a synchrotron light source to determine the chemical composition of the bead, if the pattern is natural or man-made or if the bead is genuine or a replica. These techniques revealed the bead to be composed of agate (silicon dioxide). An interesting feature on the bead’s surface was the etched rings, which were observed to contain regular copper hot spots on their circumference. Our results suggest that the Dzi bead was genuine and started out as an earth-formed agate, with the pattern crafted.
Exploring the Gender Difference and Predictors of Perceived Stress among Students Enrolled in Different Medical Programs: A Cross-Sectional Study
Authors: Infortuna Carmenrita , Gratteri Francesco , Benotakeia Andrew , Patel Sapan , Fleischman Alex , Muscatello Maria Rosaria Anna , Bruno Antonio , Zoccali Rocco Antonio , Chusid Eileen , Han Zhiyong , Battaglia Fortunato
Published: September 2020

Female medical students seem to experience higher level of perceived stress. Moreover, there is a lack of research examining perceived stress in students enrolled in different medical programs. We analyzed the association between temperament traits, optimism, self-esteem, and perceived stress of students pursuing a Doctor of Medicine (MD) degree and students pursuing a Doctor of Podiatric Medicine (DPM) degree. A cross-sectional study was conducted of two cohorts: allopathic medical students (N = 154) and the podiatric medical students (N = 150). Students anonymously completed the Perceived Stress Scale (PSS-10), Temperament Evaluation of Memphis, Pisa, Paris, and San Diego Auto Questionnaire (TEMPS-A), Rosenberg Self-Esteem Scale, and Life Orientation Test—Revised (LOT-R). We analyzed differences in the two cohort of students and predictors of perceived stress. There were no differences in the overall perception of stress between both cohorts (allopathic medical students: 18.83 ± 0.56; podiatric medical students: 19.3 ± 0.72; p = 0.4419). Women reported higher perceived stress in both programs (allopathic medical students: p = 0.0.038; podiatric medical students: p = 0.0.038). In both allopathic and podiatric medical students, the cyclothymic temperaments and anxious traits were positive predictors while hyperthymic temperaments and optimism traits were negative predictors of perceived stress. The level of perceived stress experienced by students pursuing different doctoral degrees in healthcare is similar. Regardless of the curriculum differences, female students experience higher perceived stress and there is evidence for similarities in predictors amongst allopathic and podiatric medical students.
Strengths and Weaknesses in the Risk Management of Blood-Borne Infections: Qualitative Research in Public Health
Authors: Gębska Kuczerowska Anita , Błoński Artur , Kuczerowska Joanna , Gajda Robert
Published: September 2020

This article presents the research from the first phase of our study on blood-borne risk management, wherein we solicited epidemiologists’ and healthcare practitioners’ expert opinions on a blood-borne infection risk assessment in Poland. Forty-two experts were recommended by epidemiology consultants and recruited from all districts in Poland. We used the SWOT (Strengths, Weaknesses, Opportunities, Threats) method in the evaluation. Experts’ opinions showed that there is room for improvement in the prevention of blood-borne infections. Commonly reported weaknesses in the risk assessment included gaps in knowledge and inappropriate procedures, which are largely caused by financial constraints and practitioners’ lack of awareness of developments in their trade. Strengths included legal regulations for medical services and procedures, surveillance, and increasing awareness on the part of medical staff. When paired with the existing statistical data, these results provide a comprehensive view of the problem of blood-borne infections in Poland. The analysis supported the development of a strategy proposal to prevent blood-borne infections and enhance existing risk assessment procedures.
Risk and Protective Factors of Well-Being among Healthcare Staff. A Thematic Analysis
Authors: Berlanda Sabrina , de Cordova Federica , Fraizzoli Marta , Pedrazza Monica
Published: September 2020

The purpose of this study was to identify physical and psychosocial working conditions to improve well-being at work among healthcare staff. This is a potent area of inquiry given the relationship between healthcare staff well-being and service quality and other key organizational characteristics. However, while numerous studies in this area have used a quantitative methodology, very few have applied qualitative methodologies gathering subjective descriptions of the sources of well-being, providing in so doing significant data to explore in depth the factors that influence well-being in healthcare systems. We gathered qualitative data analyzing open-ended questions about risk and protective factors of well-being at work. The sample was made of 795 professionals answering an online questionnaire. Answers were coded and analyzed using the thematic analysis with an inductive approach (data-driven). We identified four themes strongly affecting professional well-being in health-care staff: Interactions, Working Conditions, Emotional Responses to Work, and Competence and Professional Growth. Our findings suggest possible strategies and actions that may be effective in helping to calibrate case-specific support and monitoring interventions to improve health and well-being of healthcare staff. We also discuss the implications of the study and suggest possible avenues for future empirical research.
Relationships between Physical and Social Behavioural Changes and the Mental Status of Homebound Residents in Hong Kong during the COVID-19 Pandemic
Authors: Fong Ben Y. F. , Wong Martin C. S. , Law Vincent T. S. , Lo Man Fung , Ng Tommy K. C. , Yee Hilary H. L. , Leung Tiffany C. H. , Ho Percy W. T.
Published: September 2020

In Hong Kong, social distancing has been adopted in order to minimise the spread of COVID-19. This study aims to examine the changes in physical health, mental health, and social well-being experienced by local residents who were homebound during the pandemic. An online questionnaire in both Chinese and English versions was completed by 590 eligible participants from 24 April to 13 May 2020. The questionnaire found that individuals aged 18 to 25 years spent more time resting and relaxing but experienced more physical strain. Working status was associated with social contact, with participants working full-time jobs scoring higher in “maintaining social communication via electronic means” and “avoiding social activities outside the home”. Additionally, approximately one third of the participants (29.7%) had moderate to severe depression, and participants aged 18 to 25 were found to have higher scores in PHQ-9. Changes in physical health and social contact were significantly associated with developing depressive symptoms. From the results, it is clear that the COVID-19 pandemic has the potential to exert a negative impact on the mental health status of individuals.
Commentary: Physiological and Psychological Impact of Face Mask Usage during the COVID-19 Pandemic
Authors: Scheid Jennifer L. , Lupien Shannon P. , Ford Gregory S. , West Sarah L.
Published: September 2020

In this commentary, we discuss the physiological effects of wearing masks for prolonged periods of time, including special considerations, such as mask wearing among those who engage in exercise training, and concerns for individuals with pre-existing chronic diseases. In healthy populations, wearing a mask does not appear to cause any harmful physiological alterations, and the potentially life-saving benefits of wearing face masks seem to outweigh the documented discomforts (e.g. headaches). However, there continues to be controversy over mask wearing in the United States, even though wearing a mask appears to have only minor physiological drawbacks. While there are minimal physiological impacts on wearing a mask, theoretical evidence suggests that there may be consequential psychological impacts of mask wearing on the basic psychological needs of competence, autonomy, and relatedness. These psychological impacts may contribute to the controversy associated with wearing masks during the COVID-19 pandemic in the United States. After we discuss the physiological impacts of mask wearing, we will discuss psychological effects associated with wearing masks during the COVID-19 pandemic.
Spatiotemporal Distribution of Dengue and Chikungunya in the Hindu Kush Himalayan Region: A Systematic Review
Authors: Phuyal Parbati , Kramer Isabelle Marie , Klingelhöfer Doris , Kuch Ulrich , Madeburg Axel , Groneberg David A. , Wouters Edwin , Dhimal Meghnath , Müller Ruth
Published: September 2020

The risk of increasing dengue (DEN) and chikungunya (CHIK) epidemics impacts 240 million people, health systems, and the economy in the Hindu Kush Himalayan (HKH) region. The aim of this systematic review is to monitor trends in the distribution and spread of DEN/CHIK over time and geographically for future reliable vector and disease control in the HKH region. We conducted a systematic review of the literature on the spatiotemporal distribution of DEN/CHIK in HKH published up to 23 January 2020, following Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. In total, we found 61 articles that focused on the spatial and temporal distribution of 72,715 DEN and 2334 CHIK cases in the HKH region from 1951 to 2020. DEN incidence occurs in seven HKH countries, i.e., India, Nepal, Bhutan, Pakistan, Bangladesh, Afghanistan, and Myanmar, and CHIK occurs in four HKH countries, i.e., India, Nepal, Bhutan, and Myanmar, out of eight HKH countries. DEN is highly seasonal and starts with the onset of the monsoon (July in India and June in Nepal) and with the onset of spring (May in Bhutan) and peaks in the postmonsoon season (September to November). This current trend of increasing numbers of both diseases in many countries of the HKH region requires coordination of response efforts to prevent and control the future expansion of those vector-borne diseases to nonendemic areas, across national borders.
Effects of a Person-Centered Nursing Intervention for Frailty among Prefrail Community-Dwelling Older Adults
Authors: Ha Jiyeon , Park Yeon-Hwan
Published: September 2020

This study examined the effects of a person-centered nursing intervention program for frailty (PNIF) targeting community-dwelling prefrail older people in South Korea. The study participants were 40 community-dwelling older adults (≥65 years) who were classified as prefrail on the Cardiovascular Health Study (CHS) frailty index. The intervention group (n = 20) received group intervention sessions two days/week for twelve weeks and the control group (n = 20) attended lectures about frailty prevention one day/week for four weeks. The evaluation instruments included the CHS Frailty Index, a JAMAR® hydraulic hand dynamometer, the Short Physical Performance Battery (SPPB), the Korean version of the Community Healthy Activities Model Program for Seniors Questionnaire (K-CHAMPS), the Mini Nutritional Assessment (MNA), the Geriatric Depression Scale Short Form-Korea Version (GDSSF-K), the ENRICHD Social Support Instrument (ESSI), and the Goal Attainment Scale (GAS). Significant differences were found in the CHS Frailty Index (p < 0.001), left-hand grip strength (p = 0.022), right-hand grip strength (p = 0.009), SPPB (p = 0.007), K-CHAMPS (p = 0.009), MNA (p = 0.018), and GDSSF-K (p = 0.001) between the two groups after 12 weeks. No significant between-group differences in ESSI scores were observed. The PNIF effectively improved grip strength, physical function, physical activity, and nutritional status, reduced depression, and prevented frailty among community-dwelling older adults.
Eating-Disordered Behavior in Adolescents: Associations with Body Image, Body Composition and Physical Activity
Authors: Štefanová Eliška , Bakalár Peter , Baška Tibor
Published: September 2020

Eating disorders (EDs) represent a disparate group of mental health problems that significantly impair physical health or psychosocial functioning. The aim of this study was to present some evidence about the prevalence of eating-disordered behavior (EDB) in adolescents, and explore its associations with body image (BI), body composition (BC) and physical activity (PA) in this age group. Data from 780 adolescents participating in a health behavior in school-aged children (HBSC) study conducted in Slovakia in 2018 were used (mean age 13.5 ± 1.3; 56% boys). Differences in mean values of numerical indicators were evaluated using the independent samples t-test. Differences between nominal variables were assessed by the chi-square test. Pearson correlation was used to describe the associations between all the selected variables. EDB was positively screened in 26.7% (208/780) of adolescents, with a higher prevalence in girls (128/344, 37.2%) than in boys (80/436, 18.3%). Significantly higher means of BI, body weight (BW), body mass index (BMI), body fat mass (BFM), body fat percentage (BFP), body fat mass index (BFMI), fat free mass index (FFMI), and SCOFF questionnaire score (SCOFF QS) were found in those positively screened for EDB. Pearson correlation analysis revealed positive associations between EDB and BI, BW, BMI, BFM, BFP and BFMI. The prevalence of EDB is high in Slovak adolescents. Positive associations between EDB, BI, BMI and fat-related body composition parameters support the idea of a more integrated approach in EDs and obesity prevention and treatment. At the same time, gender differences suggest the need for considering gender-specific strategies aimed at girls and boys separately.
Correlation of COVID-19 Pandemic with Healthcare System Response and Prevention Measures in Saudi Arabia
Authors: Adly Heba M. , AlJahdali Imad A. , Garout Mohammed A. , Khafagy Abdullah A. , Saati Abdulla A. , Saleh Saleh A. K.
Published: September 2020

Background: The Saudi government has taken the decision to prevent the entrance of about 2.5 million international pilgrims seeking to perform hajj in order to protect the world from a catastrophic widespread of disease. Moreover, health systems in Saudi Arabia are offering free testing for residents whether Saudi and non-Saudi. Objective: This study aimed to evaluate the spread of COVID-19 associated with preventive measures taken in Saudi Arabia and to develop a detailed COVID-19 prevention strategy as a framework for the Saudi Arabia community. Methodology: Population size and age distributions among the country of Saudi Arabia were taken from the 2020 World Population Prospects. Contact patterns were measured using the Saudi Arabia Ministry of Health Statistical Annual Report. Conclusions: Our study demonstrates that performing screening tests as early as possible to facilitate the rapid detection of infected cases, fast treatment, and instant isolation for suspected cases is the most definitive rejoinder for public health. Moreover, our study revealed the significance of performing preventive measures in reducing infection and death rates around Saudi Arabia by 27%, while in other countries, it reduced the death rate ranging from 10–73%. This study provides an achievable strategy for prevention and early detection of COVID-19 spread.
Salutogenesis and Coping: Ways to Overcome Stress and Conflict
Authors: Braun-Lewensohn Orna , Mayer Claude-Hélène
Published: September 2020

This Special Issue aims to explore the concepts of stress, coping resources, and coping strategies, which are rooted in several theories, such as the stress and coping theory and the salutogenesis theory, and to understand how their core constructs are manifested in various ethnic and cultural groups around the world. This Special Issue includes 13 articles on salutogenesis and coping from different disciplinary, socio-cultural, historical, political, and economic perspectives. These articles address salutogenesis on the individual, organizational, and societal levels. The empirical studies are based in different societal and national contexts and refer to different ethnic groups within those contexts. Other studies examine international leaders in industry from a global perspective and present a systemic review of the literature concerning individuals in specific professions, such as nursing. The studies in the current Special Issue set the ground for continuing research toward even more comprehensive theoretical grounds; studies that incorporate several theoretical backgrounds and explore a broad theoretical model that may help us to understand successful adaptation in various contexts. In summary, results of studies that incorporate these theories may promote our understanding of the effects of coping resources and strategies, including acculturation strategies used among minority groups for positive adaptation.
Is Rescuer Cardiopulmonary Resuscitation Jeopardised by Previous Fatiguing Exercise?
Authors: Abraldes J. Arturo , Fernandes Ricardo J. , Rodríguez Núria , Sousa Ana
Published: September 2020

Survival outcomes increase significantly when cardiopulmonary resuscitation (CPR) is provided correctly, but rescuer’s fatigue can compromise CPR delivery. We investigated the effect of a 100-m maximal run on CPR and physiological variables in 14 emergency medical technicians (age 29.2 ± 5.8 years, height 171.2 ± 1.1 cm and weight 73.4 ± 13.1 kg). Using an adult manikin and a compression-ventilation ratio of 30:2, participants performed 4-min CPR after 4-min baseline conditions (CPR) and 4-min CPR after a 100-m maximal run carrying emergency material (CPR-run). Physiological variables were continuously measured during baseline and CPR conditions using a portable gas analyzer (K4b2, Cosmed, Rome, Italy) and analyzed using two HD video cameras (Sony, HDR PJ30VE, Japan). Higher VO2 (14.4 ± 2.1 and 22.0 ± 2.5 mL·kg−1·min−1) and heart rate (123 ± 17 and 148 ± 17 bpm) were found for CPR-run. However, the compression rate was also higher during the CPR-run (373 ± 51 vs. 340 ± 49) and between every three complete cycles (81 ± 9 vs. 74 ± 14, 99 ± 14 vs. 90 ± 10, 99 ± 10 vs. 90 ± 10, and, 101 ± 15 vs. 94 ± 11, for cycle 3, 6, 9 and 12, respectively). Fatigue induced by the 100-m maximal run had a strong impact on physiological variables, but a mild impact on CPR emergency medical technicians’ performance.
Upper Lip Horizontal Line: Characteristics of a Dynamic Facial Line
Authors: Vardimon Alexander D , Shpack Nir , Wasserstein Atalia , Skyllouriotou Marilena , Strauss Morris , Geron Silvia , Sadan Noa , Levartovsky Shifra , Sarig Rachel
Published: September 2020

Background: Upper lip appearance received major attention with the introduction of diverse treatment modalities, including lip augmentation, rhinoplasty surgery, and dental treatment designed to support the upper lip. Our objectives were to define the prevalence and characteristics of the upper lip horizontal line (ULHL), which is a dynamic line appearing during a smile, in relation to gender, malocclusions, aging, and facial morphology. Methods: First, the prevalence and gender distribution of ULHL was examined from standardized en-face imaging at full smile of 643 randomly selected patients. Second, cephalometric and dental cast model analyses were made for 97 consecutive patients divided into three age groups. Results: ULHL appears in 13.8% of the population examined, and prevailed significantly more in females (78%). The prevalence of ULHL was not related to age nor to malocclusion. Patients presenting ULHL showed shorter upper lip and deeper lip sulcus. The skeletal pattern showed longer mid-face, shorter lower facial height and greater prevalence of a gummy smile. Conclusions: Female patients with short upper lip, concavity of the upper lip, and gummy smile are more likely to exhibit ULHL. The ULHL is not age-related and can be identified in children and young adults. Therefore, it should be considered when selecting diverse treatment modalities involving the upper lip.
Red and Processed Meat Consumption and Risk of Depression: A Systematic Review and Meta-Analysis
Authors: Nucci Daniele , Fatigoni Cristina , Amerio Andrea , Odone Anna , Gianfredi Vincenza
Published: September 2020

Depression is one of the leading causes of disability worldwide, with more than 264 million people affected. On average, depression first appears during the late teens to mid-20s as result of a complex interaction of social, psychological and biological factors. The aim of this systematic review with meta-analysis is to assess the association between red and processed meat intake and depression (both incident and prevalent). This systematic review was conducted according to the methods recommended by the Cochrane Collaboration and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Relevant papers published through March 2020 were identified by searching the electronic databases MEDLINE, Embase and Scopus. All analyses were conducted using ProMeta3 software. A critical appraisal was conducted. Finally, 17 studies met the inclusion criteria. The overall effect size (ES) of depression for red and processed meat intake was 1.08 [(95% CI = 1.04; 1.12), p-value < 0.001], based on 241,738 participants. The results from our meta-analysis showed a significant association between red and processed meat intake and risk of depression. The presented synthesis will be useful for health professionals and policy makers to better consider the effect of diet on mental health status.
Social Distancing Compliance under COVID-19 Pandemic and Mental Health Impacts: A Population-Based Study
Authors: Zhao Sheng Zhi , Wong Janet Yuen Ha , Wu Yongda , Choi Edmond Pui Hang , Wang Man Ping , Lam Tai Hing
Published: September 2020

The success of public health measures for controlling the coronavirus disease 2019 (COVID-19) pandemic relies on population compliance. We analyzed compliance with social distancing and its associations with mental health. The Hong Kong COVID-19 Health Information Survey was conducted from 9–23 April 2020 on 1501 adults randomly sampled for landline telephone interviews (n = 500) and online surveys (n = 1001). Compliance with social distancing and staying-at-home, stress (Perceived Stress Scale-4), anxiety (General Anxiety Disorders-2), and depressive symptoms (Patient Health Questionnaire-2) were collected. The associations between mental health symptoms and compliance were examined by multivariable regression models. Of the 1501 respondents (52.5% female, 72.3% aged 18–59 years), 74.2%, 72.7%, and 59.7% reported avoiding going out, going to crowded places, and attending social gatherings of more than four people, respectively. Most respondents had stayed-at-home for at least four of the past seven days (58.4%; mean 4.12, Standard Deviation 2.05). Adoption, perceived effectiveness, and perceived compliance with social distancing were associated with lower stress levels and less anxiety and depressive symptoms (all p < 0.01). However, more days stayed-at-home were associated with more depressive symptoms (adjusted Odds Ratio 1.09; 95%Confidence Interval 1.00, 1.18). The long-term psychological impact in relation to social distancing and staying-at-home requires further investigation.
Identification and Geographic Distribution of Accommodation and Catering Centers
Authors: Han Ze , Song Wei
Published: September 2020

As the most important manifestation of the activities of the life service industry, the reasonable layout of spatial agglomeration and dispersion of the accommodation and catering industry plays an important role in guiding the spatial structure of the urban industry and population. Applying the contour tree and location quotient index methods, based on points of interest (POI) data of the accommodation and catering industry in Beijing and on the identification of the spatial structure and cluster center of the accommodation and catering industry, we investigated the distribution and agglomeration characteristics of the urban accommodation and catering industry from the perspective of industrial spatial differentiation. The results show that: (1) the accommodation and catering industry in Beijing presents a polycentric agglomeration pattern in space, mainly distributed within a radius of 20 km from the city center and on a relatively large scale; areas beyond this distance contain isolated single cluster centers. (2) From the perspective of the industry, the cluster centers close to the core area of the city are characterized by the agglomeration of multiple advantageous industries, while those in the outer suburbs of the city are more prominent in a single industry. (3) From the perspective of the location quotient of cluster centers, the leisure catering industries are mainly located close to the urban centers. On the contrary, the cluster centers in the outer suburbs and counties are relatively small and dominated by restaurants and fast food industries. Commercial accommodation businesses are mainly distributed in the transportation hub centers and in entertainment and leisure areas.
A Spatial Agent-Based Model to Assess the Spread of Malaria in Relation to Anti-Malaria Interventions in Southeast Iran
Authors: Gharakhanlou Navid Mahdizadeh , Hooshangi Navid , Helbich Marco
Published: September 2020

Malaria threatens the lives of many people throughout the world. To counteract its spread, knowledge of the prevalence of malaria and the effectiveness of intervention strategies is of great importance. The aim of this study was to assess (1) the spread of malaria by means of a spatial agent-based model (ABM) and (2) the effectiveness of several interventions in controlling the spread of malaria. We focused on Sarbaz county in Iran, a malaria-endemic area where the prevalence rate is high. Our ABM, which was carried out in two steps, considers humans and mosquitoes along with their attributes and behaviors as agents, while the environment is made up of diverse environmental factors, namely air temperature, relative humidity, vegetation, altitude, distance from rivers and reservoirs, and population density, the first three of which change over time. As control interventions, we included long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS). The simulation results showed that applying LLINs and IRS in combination, rather than separately, was most efficient in reducing the number of infected humans. In addition, LLINs and IRS with moderate or high and high coverage rates, respectively, had significant effects on reducing the number of infected humans when applied separately. Our results can assist health policymakers in selecting appropriate intervention strategies in Iran to reduce malaria transmission.
Functional Characterization of Muscarinic Receptors in Human Schwann Cells
Authors: Piovesana Roberta , Faroni Alessandro , Tata Ada Maria , Reid Adam J.
Published: September 2020

Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of this receptor subtype inhibits SC proliferation, improving their differentiation towards myelinating phenotype. In this work, we describe for the first time that human SCs are cholinoceptive as they express several muscarinic receptor subtypes and, as for rat SCs, M2 receptor is one of the most abundant. Human SCs, isolated from adult nerves, were cultured in vitro and stimulated with M2 muscarinic agonist arecaidine propargyl ester (APE). Similarly to that observed in rat, M2 receptor activation causes a decreased cell proliferation and promotes SC differentiation as suggested by increased Egr2 expression with an improved spindle-like shape cell morphology. Conversely, the non-selective stimulation of muscarinic receptors appears to promote cell proliferation with a reduction of SC average cell diameter. The data obtained demonstrate that human SCs are cholinoceptive and that human cultured SCs may represent an interesting tool to understand their physiology and increase the knowledge on how the cholinergic stimulation may contribute to address human SC development in normal and pathological conditions.
Relative and Absolute Quantification of Aberrant and Normal Splice Variants in HBBIVSI−110 (G > A) β-Thalassemia
Authors: Patsali Petros , Papasavva Panayiota , Christou Soteroulla , Sitarou Maria , Antoniou Michael N. , Lederer Carsten W. , Kleanthous Marina
Published: September 2020

The β-thalassemias are an increasing challenge to health systems worldwide, caused by absent or reduced β-globin (HBB) production. Of particular frequency in many Western countries is HBBIVSI−110(G > A) β-thalassemia (HGVS name: HBB:c.93-21G > A). Its underlying mutation creates an abnormal splice acceptor site in the HBB gene, and while partially retaining normal splicing of HBB, it severely reduces HBB protein expression from the mutant locus and HBB loci in trans. For the assessment of the underlying mechanisms and of therapies targeting β-thalassemia, accurate quantification of aberrant and normal HBB mRNA is essential, but to date, has only been performed by approximate methods. To address this shortcoming, we have developed an accurate, duplex reverse-transcription quantitative PCR assay for the assessment of the ratio and absolute quantities of normal and aberrant mRNA species as a tool for basic and translational research of HBBIVSI−110(G > A) β-thalassemia. The method was employed here to determine mRNA ratios and quantities in blood and primary cell culture samples and correlate them with HBB protein levels. Moreover, with its immediate utility for β-thalassemia and the mutation in hand, the approach can readily be adopted for analysis of alternative splicing or for quantitative assays of any disease-causing mutation that interferes with normal splicing.
Engineered Fragments of the PSMA-Specific 5D3 Antibody and Their Functional Characterization
Authors: Novakova Zora , Belousova Nikola , Foss Catherine A. , Havlinova Barbora , Gresova Marketa , Das Gargi , Lisok Ala , Prada Adam , Barinkova Marketa , Hubalek Martin , Pomper Martin G. , Barinka Cyril
Published: September 2020

Prostate-Specific Membrane Antigen (PSMA) is an established biomarker for the imaging and experimental therapy of prostate cancer (PCa), as it is strongly upregulated in high-grade primary, androgen-independent, and metastatic lesions. Here, we report on the development and functional characterization of recombinant single-chain Fv (scFv) and Fab fragments derived from the 5D3 PSMA-specific monoclonal antibody (mAb). These fragments were engineered, heterologously expressed in insect S2 cells, and purified to homogeneity with yields up to 20 mg/L. In vitro assays including ELISA, immunofluorescence and flow cytometry, revealed that the fragments retain the nanomolar affinity and single target specificity of the parent 5D3 antibody. Importantly, using a murine xenograft model of PCa, we verified the suitability of fluorescently labeled fragments for in vivo imaging of PSMA-positive tumors and compared their pharmacokinetics and tissue distribution to the parent mAb. Collectively, our data provide an experimental basis for the further development of 5D3 recombinant fragments for future clinical use.
Comment on: Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. Int. J. Mol. Sci. 2020, 21, 4661
Authors: Pang Kok-Lun , Chin Kok-Yong
Published: September 2020

Dear Editor, [...]
Dihydromyricetin Improves Endothelial Dysfunction in Diabetic Mice via Oxidative Stress Inhibition in a SIRT3-Dependent Manner
Authors: Hua Yu-Yun , Zhang Yue , Gong Wei-Wei , Ding Yue , Shen Jie-Ru , Li Hua , Chen Yun , Meng Guo-Liang
Published: September 2020

Dihydromyricetin (DHY), a flavonoid component isolated from Ampelopsis grossedentata, exerts versatile pharmacological activities. However, the possible effects of DHY on diabetic vascular endothelial dysfunction have not yet been fully elucidated. In the present study, male C57BL/6 mice, wild type (WT) 129S1/SvImJ mice and sirtuin 3 (SIRT3) knockout (SIRT3-/-) mice were injected with streptozotocin (STZ, 60 mg/kg/day) for 5 consecutive days. Two weeks later, DHY were given at the doses of 250 mg/kg by gavage once daily for 12 weeks. Fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c) level, endothelium-dependent relaxation of thoracic aorta, reactive oxygen species (ROS) production, SIRT3, and superoxide dismutase 2 (SOD2) protein expressions, as well as mitochondrial Deoxyribonucleic Acid (mtDNA) copy number, in thoracic aorta were detected. Our study found that DHY treatment decreased FBG and HbA1c level, improved endothelium-dependent relaxation of thoracic aorta, inhibited oxidative stress and ROS production, and enhanced SIRT3 and SOD2 protein expression, as well as mtDNA copy number, in thoracic aorta of diabetic mice. However, above protective effects of DHY were unavailable in SIRT3-/- mice. The study suggested DHY improved endothelial dysfunction in diabetic mice via oxidative stress inhibition in a SIRT3-dependent manner.
The Expression Profile of mRNA and tRNA Genes in Splenocytes and Neutrophils after In Vivo Delivery of Antitumor Short Hairpin RNA of Indoleamine 2,3- Dioxygenase
Authors: Huang Ming-Shyan , Hsu Ya-Ling , Yeh I-Jeng , Liu Kuan-Ting , Yen Meng-Chi
Published: September 2020

RNA-based therapeutics are considered as novel treatments for human diseases. Our previous study demonstrated that treatment with short-hairpin RNA against Ido1 (IDO shRNA) suppresses tumor growth, detects Th1-bias immune responses, and elevates expression of tryptophan transfer RNA (tRNATrp) in total splenocytes. In addition, depletion of Ly6g+ neutrophils attenuates the effect of IDO shRNA. The aim of this study was to investigate the regulatory network and the expression profile of tRNAs and other non-coding RNAs in IDO shRNA-treated spleens. The total splenocytes and magnetic bead-enriched splenic neutrophils were collected from the lung tumor bearing mice, which were treated with IDO shRNA or scramble IDO shRNA, and the collected cells were subsequently subjected to RNA sequencing. The gene ontology analysis revealed the different enrichment pathways in total splenocytes and splenic neutrophils. Furthermore, the expression of tRNA genes was identified and validated. Six isoacceptors of tRNA, with different expression patterns between total splenocytes and splenic neutrophils, were observed. In summary, our findings not only revealed novel biological processes in IDO shRNA-treated total splenocytes and splenic neutrophils, but the identified tRNAs and other non-coding RNAs may contribute to developing a novel biomarker gene set for evaluating the clinical efficiency of RNA-based cancer immunotherapies.
Mismatch-Repair Protein Expression in High-Grade Gliomas: A Large Retrospective Multicenter Study
Authors: Caccese Mario , Ius Tamara , Simonelli Matteo , Fassan Matteo , Cesselli Daniela , Dipasquale Angelo , Cavallin Francesco , Padovan Marta , Salvalaggio Alessandro , Gardiman Marina Paola , Skrap Miran , Zagonel Vittorina , Lombardi Giuseppe
Published: September 2020

Background: DNA mismatch repair (MMR) is a system for repairing errors in DNA replication. Cancer cells with MMR deficiency can have immunohistochemical loss of MMR protein expression leading to a hypermutable phenotype that may correlate with anti-PD1 efficacy. Scant data exist about immunohistochemical loss of MMR protein expression in high-grade gliomas (HGG). Materials and Methods: We performed a large multicenter retrospective study to investigate the frequency and the prognostic role of immunohistochemical loss of MMR protein expression in HGG patients; we nevertheless evaluated the association between this status and clinical or molecular characteristics. Immunohistochemical loss of MMR protein expression was recorded as partial or complete loss of at least 1 MMR protein. Results: We analyzed the expression of MMR proteins in tumor tissue of 355 consecutive patients. Partial and complete immunohistochemical loss of MMR proteins was found in 43/355 samples (12.1%) and among these, 15 cases (4.2%) showed a complete loss of at the least one MMR protein. Alteration of MSH2 expression was found in 55.8%, MSH6 in 46.5%, PMS2 in 34.9%, and MLH1 in 30.2%. Alteration of MMR protein expression was statistically more frequent in anaplastic gliomas, in recurrent disease, in patients treated with temozolomide, and in IDH-mut gliomas. Immunohistochemical loss of MMR proteins was not associated with survival, adjusting for clinically relevant confounders. Conclusions: MMR protein expression status did not affect survival in HGG patients. We identified clinical and molecular characteristics correlating with immunohistochemical loss of MMR proteins expression. A large study should be performed to analyze its predictive role of immune checkpoint inhibitor efficacy in these subgroups of patients.