bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Characterization of MyD88 in Anodonta woodiana and Its Involvement in the Innate Immune Response to Bacterial Infection
Authors: Qu Fufa , She Qing , Li Jialing , Zeng Xuan , Li Yumiao , Liu Xinyu , Ren Lingxin , Liu Zhenzhen , Gao Chaoran , Lu Xinyu , Long Mengyao , Li Xinya
Published: June 2022

Myeloid differentiation factor 88 (MyD88) is a key adapter molecule in Toll-like receptor signal transduction that triggers downstream immune cascades involved in the host defense response to exogenous pathogens. However, the function of MyD88s in mollusks, especially in freshwater shellfish, remains poorly understood. In this study, a novel freshwater shellfish MyD88 (denoted AwMyD88) was characterized from Anodonta woodiana. The present AwMyD88 protein consists of 474 amino acids and contains a conserved a typical death domain (DD) and a conservative Toll/IL-1R (TIR) domain with three typical boxes. Quantitative real-time PCR (qRT-PCR) analysis showed that AwMyD88 was broadly expressed in all the examined tissues, and the highest expression level was observed in hemocytes of A. woodiana. When challenged with Aeromonas hydrophila and lipopolysaccharide (LPS), the mRNA expression levels of AwMyD88 were significantly induced in hemocytes of A. woodiana in vivo and in vitro. In addition, in vivo injection experiments revealed that MyD88 signaling pathway genes showed strong responsiveness to A. hydrophila challenge, and their expression levels were significantly upregulated in hemocytes. Knockdown of AwMyD88 reduced the transcript levels of immune related transcription factors (AwNF-κB and AwAP-1) and effectors (AwTNF, AwLYZ, AwDefense and AwAIF) during A. hydrophila infection. Moreover, subcellular localization analysis indicated that AwMyD88 was mainly localized to the cytoplasm in HEK293T cells. Finally, luciferase reporter assays revealed that AwMyD88 associates with AwTLR to activate the NF-κB and AP-1 signaling pathways in HEK293T cells. These results suggested that AwMyD88 might be involved in the host defense response to bacterial challenge, providing new insight into the immune function of the MyD88 signaling pathway in freshwater shellfish.
Endothelial Dysfunction in Psoriasis: An Updated Review
Authors: Anyfanti Panagiota , Margouta Anastasia , Goulas Kyriakos , Gavriilaki Maria , Lazaridou Elizabeth , Patsatsi Aikaterini , Gkaliagkousi Eugenia
Published: June 2022

Although psoriasis is predominantly a chronic inflammatory skin disorder, epidemiological data provide a solid link between psoriasis, especially in its more severe forms, and increased risk for cardiovascular morbidity and mortality. Apart from the increased prevalence of traditional cardiovascular risk factors, chronic inflammation appears to act synergistically with the underlying process of endothelial dysfunction toward the development of accelerated atherosclerosis, subclinical vascular injury and subsequently, clinically evident cardiovascular manifestations. Endothelial dysfunction is regarded as an early precursor of atherosclerosis with a predictive value for the development of future cardiovascular events. A thorough understanding of the mechanisms of endothelial dysfunction in psoriasis might pave the path for the development of more accurate cardiovascular risk prediction tools and possible therapeutic targets aiming to alleviate the increased cardiovascular burden associated with the disease. The present review summarizes the available evidence about the role of chronic inflammation and other important pathophysiological mechanisms involved in the development of endothelial dysfunction in psoriasis. An overview of studies implementing the most widely applied circulating and vascular biomarkers of endothelial dysfunction in psoriasis patients will be provided, and the impact of systemic psoriasis treatments on endothelial dysfunction and patients’ cardiovascular risk will be discussed.
Prevalence and Clinical Characteristics of Calciphylaxis in Chinese Hemodialysis Patients
Authors: Liu Yuqiu , Yang Canlin , Yang Xin , Xie Xiaotong , Liu Hong , Zhang Liuping , Ye Jianming , Jiang Dongsheng , Zhang Xiaoliang , Liu Bicheng
Published: June 2022

Background

Calciphylaxis is a grievous life-threatening vascular disease that commonly affects dialysis population. This is the first epidemiological survey of calciphylaxis initiated in China.

Methods

In the cross-sectional survey, a stratified sampling method was used to select 24 dialysis centers in Jiangsu Province. The participants were all adult patients in each center who had been on hemodialysis for more than 6 months. Calciphylaxis patients were uniformly diagnosed based on characteristic skin lesions and histopathological features.

Results

A total of 3,867 hemodialysis patients (average age of 55.33 ± 13.89 years; 61.81% of males) were included. Forty eight cases were diagnosed with calciphylaxis, and prevalence was 1.24%. Among calciphylaxis patients, 33 cases were male, and the average age and median dialysis duration were 53.85 ± 15.17 years and 84.00 (48.00, 138.75) months, respectively. Skin biopsy was performed in 70.83% of calciphylaxis patients, and positive rate was 64.71%. Meanwhile, the positive rate of bone scintigraphy in the diagnosis of calciphylaxis was 62.5%. The prevalence of hyperparathyroidism in case group was as high as 72.92% with longer duration, and 42.86% had undergone parathyroidectomy. Multivariate analysis indicated that increased BMI, prolonged dialysis duration, warfarin therapy, hyperparathyroidism, diabetes, tumors, low serum albumin and high serum alkaline phosphatase levels were high-risk factors for calciphylaxis.

Conclusions

The prevalence of calciphylaxis in Chinese hemodialysis patients was 1.24% according to regional epidemiological survey, but its actual prevalence would be presumably far beyond present data. It's urgent to improve clinical understanding of calciphylaxis, and multifaceted diagnostic methods should be applied for early screening.
Pulmonary Actinomyces graevenitzii Infection: Case Report and Review of the Literature
Authors: Yuan Yuan , Hou Ziliang , Peng Dan , Xing Zhenchuan , Wang Jinxiang , Zhang Shuai
Published: June 2022

Background

Pulmonary actinomycosis (PA), a chronic indolent infection, is a diagnostic challenge. Actinomyces graevenitzii is a relatively rare Actinomyces species isolated from various clinical samples.

Case Presentation

A 47-year-old patient presented with a 3-month history of mucopurulent expectoration and dyspnea and a 3-day history of fever up to 39.0°C. He had dental caries and a history of alcoholism. Computed tomography (CT) images of the chest revealed a consolidation shadow in the right upper and middle lobes, with necrosis containing foci of air. Actinomyces graevenitzii was isolated from bronchoalveolar lavage fluid (BALF) culture and was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. He received treatment with intravenous piperacillin-sulbactam for 10 days and oral amoxicillin-clavulanate for 7 months. His clinical condition had considerably improved. The consolidation shadow was gradually absorbed.

Conclusion

Early diagnosis and treatment of pulmonary actinomycosis are crucial. Bronchoscopy plays a key role in the diagnostic process, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) is an accurate tool for Actinomyces identification.
Sophoridine Suppresses Herpes Simplex Virus Type 1 Infection by Blocking the Activation of Cellular PI3K/Akt and p38 MAPK Pathways
Authors: Tang Qiong , Luan Fei , Yuan An , Sun Jiayi , Rao Zhili , Wang Baojun , Liu Yao , Zeng Nan
Published: June 2022

Herpes simplex virus type 1 (HSV-1) is a ubiquitous and important human pathogen capable of causing significant clinical diseases ranging from skin damage to encephalitis, particularly in immunocompromised and neonatal hosts. Currently, widely used nucleoside analogs, including acyclovir and penciclovir, have some limitations in their use due to side effects and drug resistance. Herein, we report sophoridine's (SRI) dramatic inhibition of HSV-1 replication in vitro. SRI exhibited a remarkable inhibitory influence on HSV-1 virus-induced cytopathic effect and plaque formation, as well as on progeny viruses in Vero and HeLa cells, with selection indexes (SI) of 38.96 and 22.62, respectively. Moreover, SRI also considerably suppressed HSV-1 replication by hindering the expression of viral immediate-early (ICP0 and ICP22), early (ICP8 and TK), and late (gB and gD) genes and the expression of viral proteins ICP0, gB, and gD. We suggest that SRI can directly inactivate viral particles and block some stages in the life cycle of HSV-1 after adsorption. Further experiments showed that SRI downregulated the cellular PI3K/Akt signaling pathway and obstructed HSV-1 replication even more. Most importantly, SRI markedly repressed HSV-1-induced p38 MAPK pathway activation. Collectively, this natural bioactive alkaloid could be a promising therapeutic candidate against HSV-1 via the modulation of cellular PI3K/Akt and p38 MAPK pathways.
Perfusion Imaging of Fatigue and Time-on-Task Effects in Patients With Parkinson’s Disease
Authors: Liu Wanting , Liu Jianghong , Bhavsar Rupal , Mao Tianxin , Mamikonyan Eugenia , Raizen David , Detre John A. , Weintraub Daniel , Rao Hengyi
Published: June 2022

Fatigue is a highly prevalent and debilitating non-motor symptom in Parkinson’s disease (PD), yet its’ neural mechanisms remain poorly understood. Here we combined arterial spin labeling (ASL) perfusion functional magnetic resonance imaging (fMRI) with a sustained mental workload paradigm to examine the neural correlates of fatigue and time-on-task effects in PD patients. Twenty-one PD patients were scanned at rest and during continuous performance of a 20-min psychomotor vigilance test (PVT). Time-on-task effects were measured by the reaction time changes during the PVT and by self-reported fatigue ratings before and after the PVT. PD subjects demonstrated significant time-on-task effects, including progressively slower reaction time on the PVT and increased post-PVT fatigue ratings compared to pre-PVT. Higher levels of general fatigue were associated with larger increases in mental fatigue ratings after the PVT. ASL imaging data showed increased CBF in the right middle frontal gyrus (MFG), bilateral occipital cortex, and right cerebellum during the PVT compared to rest, and decreased CBF in the right MFG at post-task rest compared to pre-task rest. The magnitude of regional CBF changes in the right MFG and right inferior parietal lobe correlated with subjective fatigue rating increases after the PVT task. These results demonstrate the utility of continuous PVT paradigm for future studies of fatigue and cognitive fatigability in patients, and support the key role of the fronto-parietal attention network in mediating fatigue in PD.
Calculating and Comparing the Annualized Relapse Rate and Estimating the Confidence Interval in Relapsing Neurological Diseases
Authors: Akaishi Tetsuya , Ishii Tadashi , Aoki Masashi , Nakashima Ichiro
Published: June 2022

Calculating the crude or adjusted annualized relapse rate (ARR) and its confidence interval (CI) is often required in clinical studies to evaluate chronic relapsing diseases, such as multiple sclerosis and neuromyelitis optica spectrum disorders. However, accurately calculating ARR and estimating the 95% CI requires careful application of statistical approaches and basic familiarity with the exponential family of distributions. When the relapse rate can be regarded as constant over time or by individuals, the crude ARR can be calculated using the person-years method, which divides the number of all observed relapses among all participants by the total follow-up period of the study cohort. If the number of relapses can be modeled by the Poisson distribution, the 95% CI of ARR can be obtained by finding the 2.5% upper and lower critical values of the parameter λ as the mean. Basic familiarity with F-statistics is also required when comparing the ARR between two disease groups. It is necessary to distinguish the observed relapse rate ratio (RR) between two sample groups (sample RR) from the unobserved RR between their originating populations (population RR). The ratio of population RR to sample RR roughly follows the F distribution, with degrees of freedom obtained by doubling the number of observed relapses in the two sample groups. Based on this, a 95% CI of the population RR can be estimated. When the count data of the response variable is overdispersed, the negative binomial distribution would be a better fit than the Poisson. Adjusted ARR and the 95% CI can be obtained by using the generalized linear regression models after selecting appropriate error structures (e.g., Poisson, negative binomial, zero-inflated Poisson, and zero-inflated negative binomial) according to the overdispersion and zero-inflation in the response variable.
The Clinical Impact of Methotrexate-Induced Stroke-Like Neurotoxicity in Paediatric Departments: An Italian Multi-Centre Case-Series
Authors: Santangelo Andrea , Bartolini Emanuele , Nuzzi Giulia , Foiadelli Thomas , Michev Alexandre , Mina Tommaso , Trambusti Irene , Fichera Valeria , Bonuccelli Alice , Massimetti Gabriele , Peroni Diego G. , De Marco Emanuela , Coccoli Luca , Luti Laura , Bernasconi Sayla , Nardi Margherita , Menconi Maria Cristina , Casazza Gabriella , Pruna Dario , Mura Rosamaria , Marra Chiara , Zama Daniele , Striano Pasquale , Cordelli Duccio M. , Battini Roberta , Orsini Alessandro
Published: June 2022

Introduction

Stroke-like syndrome (SLS) is a rare subacute neurological complication of intrathecal or high-dose (≥500 mg) Methotrexate (MTX) administration. Its clinical features, evoking acute cerebral ischaemia with fluctuating course symptoms and a possible spontaneous resolution, have elicited interest among the scientific community. However, many issues are still open on the underlying pathogenesis, clinical, and therapeutic management and long-term outcome.

Materials and Methods

We retrospectively analyzed clinical, radiological and laboratory records of all patients diagnosed with SLS between 2011 and 2021 at 4 National referral centers for Pediatric Onco-Hematology. Patients with a latency period that was longer than 3 weeks between the last MTX administration of MTX and SLS onset were excluded from the analysis, as were those with unclear etiologies. We assessed symptom severity using a dedicated arbitrary scoring system. Eleven patients were included in the study.

Results

The underlying disease was acute lymphoblastic leukemia type B in 10/11 patients, while fibroblastic osteosarcoma was present in a single subject. The median age at diagnosis was 11 years (range 4–34), and 64% of the patients were women. Symptoms occurred after a mean of 9.45 days (± 0.75) since the last MTX administration and lasted between 1 and 96 h. Clinical features included hemiplegia and/or cranial nerves palsy, paraesthesia, movement or speech disorders, and seizure. All patients underwent neuroimaging studies (CT and/or MRI) and EEG. The scoring system revealed an average of 4.9 points (± 2.3), with a median of 5 points (maximum 20 points). We detected a linear correlation between the severity of the disease and age in male patients.

Conclusions

SLS is a rare, well-characterized complication of MTX administration. Despite the small sample, we have been able to confirm some of the previous findings in literature. We also identified a linear correlation between age and severity of the disease, which could improve the future clinical management.
A Regional Smoothing Block Sparse Bayesian Learning Method With Temporal Correlation for Channel Selection in P300 Speller
Authors: Zhao Xueqing , Jin Jing , Xu Ren , Li Shurui , Sun Hao , Wang Xingyu , Cichocki Andrzej
Published: June 2022

The P300-based brain–computer interfaces (BCIs) enable participants to communicate by decoding the electroencephalography (EEG) signal. Different regions of the brain correspond to various mental activities. Therefore, removing weak task-relevant and noisy channels through channel selection is necessary when decoding a specific type of activity from EEG. It can improve the recognition accuracy and reduce the training time of the subsequent models. This study proposes a novel block sparse Bayesian-based channel selection method for the P300 speller. In this method, we introduce block sparse Bayesian learning (BSBL) into the channel selection of P300 BCI for the first time and propose a regional smoothing BSBL (RSBSBL) by combining the spatial distribution properties of EEG. The RSBSBL can determine the number of channels adaptively. To ensure practicality, we design an automatic selection iteration strategy model to reduce the time cost caused by the inverse operation of the large-size matrix. We verified the proposed method on two public P300 datasets and on our collected datasets. The experimental results show that the proposed method can remove the inferior channels and work with the classifier to obtain high-classification accuracy. Hence, RSBSBL has tremendous potential for channel selection in P300 tasks.
Artificial Intelligence–Assisted Endoscopic Diagnosis of Early Upper Gastrointestinal Cancer: A Systematic Review and Meta-Analysis
Authors: Luo De , Kuang Fei , Du Juan , Zhou Mengjia , Liu Xiangdong , Luo Xinchen , Tang Yong , Li Bo , Su Song
Published: June 2022

Objective

The aim of this study was to assess the diagnostic ability of artificial intelligence (AI) in the detection of early upper gastrointestinal cancer (EUGIC) using endoscopic images.

Methods

Databases were searched for studies on AI-assisted diagnosis of EUGIC using endoscopic images. The pooled area under the curve (AUC), sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) with 95% confidence interval (CI) were calculated.

Results

Overall, 34 studies were included in our final analysis. Among the 17 image-based studies investigating early esophageal cancer (EEC) detection, the pooled AUC, sensitivity, specificity, PLR, NLR, and DOR were 0.98, 0.95 (95% CI, 0.95–0.96), 0.95 (95% CI, 0.94–0.95), 10.76 (95% CI, 7.33–15.79), 0.07 (95% CI, 0.04–0.11), and 173.93 (95% CI, 81.79–369.83), respectively. Among the seven patient-based studies investigating EEC detection, the pooled AUC, sensitivity, specificity, PLR, NLR, and DOR were 0.98, 0.94 (95% CI, 0.91–0.96), 0.90 (95% CI, 0.88–0.92), 6.14 (95% CI, 2.06–18.30), 0.07 (95% CI, 0.04–0.11), and 69.13 (95% CI, 14.73–324.45), respectively. Among the 15 image-based studies investigating early gastric cancer (EGC) detection, the pooled AUC, sensitivity, specificity, PLR, NLR, and DOR were 0.94, 0.87 (95% CI, 0.87–0.88), 0.88 (95% CI, 0.87–0.88), 7.20 (95% CI, 4.32–12.00), 0.14 (95% CI, 0.09–0.23), and 48.77 (95% CI, 24.98–95.19), respectively.

Conclusions

On the basis of our meta-analysis, AI exhibited high accuracy in diagnosis of EUGIC.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier PROSPERO (CRD42021270443).
The Applicability of the Results in the Asian Population of ORIENT-11 to a Western Population According to the ICH-E5 Framework
Authors: Liu Stephen V. , Nagasaka Misako , Stefaniak Victoria , Gruver Kristi , Lin Yong , Ferry David , Socinski Mark A. , Zhang Li
Published: June 2022

Sintilimab combined with pemetrexed and platinum met the primary endpoint of improving progression-free survival (PFS) as a first-line therapy for nonsquamous non-small cell lung cancer (NSCLC) in the phase 3 trial ORIENT-11 (NCT03607539). As seen in similar trials, the addition of sintilimab, a PD-1 inhibitor, to chemotherapy improved the PFS without significantly worsening the toxicity, with improvements in response rate and duration of response. In contrast to previous trials, the ORIENT-11 trial was conducted completely in China. Both intrinsic and extrinsic factors are important to consider when reviewing foreign clinical trial data, as they may influence the efficacy and the safety outcomes. Here we discuss the applicability of ORIENT-11 clinical results to a Western population.
SARS-CoV-2 Associated Pediatric Inflammatory Multisystem Syndrome With a High Prevalence of Myocarditis – A Multicenter Evaluation of Clinical and Laboratory Characteristics, Treatment and Outcome
Authors: Thom Katharina , Kahl Beatrice , Wagner Thomas , van Egmond-Fröhlich Andreas , Krainz Mathias , Frischer Thomas , Leeb Iris , Schuster Christine , Ehringer-Schetitska Doris , Minkov Milen , Male Christoph , Michel-Behnke Ina
Published: June 2022

Introduction

Pediatric inflammatory multisystem syndrome – temporally associated with SARS-CoV-2 infection (PIMS –TS) comprises a new disease entity having emerged after the COVID-19 outbreak in 2019.

Materials and Methods

For this multicenter, retrospective study children between 0 and 18 years with PIMS-TS between March 2020 and May 2021 were included, before availability of vaccination for children. Frequent SARS-CoV-2 variants at that period were the wildtype virus, alpha, beta and delta variants. Inclusion criteria were according to the PIMS-TS criteria, proposed by the Royal College of Pediatrics and WHO. Study aim was to review their clinical, laboratory and echocardiographic data with a focus on cardiac involvement.

Results

We report 45 patients, median age 9 years, 64% male. SARS-CoV-2 antibodies were positive in 35/41 (85%). PIMS occurrence followed local COVID-19 peak incidence periods with a time lag. The most common symptoms at presentation were fever (98%), abdominal pain (89%) and rash (80%). Fever history of > 5 days was associated with decreased left ventricular function (p = 0.056). Arterial hypotension and cardiac dysfunction were documented in 72% patients, increased brain natriuretic peptide in 96% and increased cardiac troponin in 64% of the children. Echocardiography revealed mitral valve regurgitation (64%), coronary abnormalities (36%) and pericardial effusions (40%). Increased NT-proBNP was significantly associated with the need of inotropics (p < 0.05), which were necessary in 40% of the patients. Treatment comprised intravenous immunoglobulin (93%), systemic steroids (84%) and acetylsalicylic acid (100%; 26/45 started with high dosages). For insufficient response to this treatment, five (11%) children received the interleukin-1 receptor antagonist anakinra. All patients were discharged with almost resolved cardiac signs.

Conclusion

Our analysis of non-vaccinated children with PIMS-TS demonstrates that a considerable number have associated myocarditis requiring intensive care and inotropic support. Most children showed adequate response to intravenous immunoglobulin and steroids and good recovery. Further evaluation of pediatric patients with COVID-19 associated diseases is required to evaluate the impact of new virus variants.
Impact of 11q Loss of Heterozygosity Status on the Response of High-Risk Neuroblastoma With MYCN Amplification to Neoadjuvant Chemotherapy
Authors: Lu Xian-Ying , Qu Li-Jun , Duan Xian-Lun , Zuo Wei , Sai Kai , Rui Gang , Gong Xian-Feng , Ding Yi-bo , Gao Qun
Published: June 2022

Purpose

The aim of this study was to investigate whether 11q loss of heterozygosity (LOH) aberration would impact the response of the primary tumor to neoadjuvant chemotherapy or to the degree of surgical resection in neuroblastoma (NB) patients with MYCN amplification.

Methods

The clinical data of 42 NB patients with MYCN amplification who were newly diagnosed and received treatments at our hospital from 2011 to 2020 were retrospectively analyzed. According to the results of the segmental chromosome aberration analysis, the patients enrolled were assigned to an 11qLOH positive group and an 11qLOH negative group.

Results

There was no significant difference in the mean number of chemotherapy courses completed before surgery between the 11qLOH positive and 11qLOH negative groups (p = 0.242). Each of the 42 patients had metaiodobenzylguanidine (MIBG) scans both before and after neoadjuvant chemotherapy. The percentage of patients who had a clinical MIBG change in the 11qLOH positive group was lower than the percentage in the 11qLOH negative group (27.27 vs. 66.67%, p = 0.030). The 11qLOH negative group seemed to have a higher rate of surgical resection (≥90%); however, the difference between the two groups was not statistically significant (p = 0.088). Furthermore, the 11qLOH negative group did not show significantly superior event-free survival and overall survival rates compared with the 11qLOH positive group.

Conclusions

This study showed that patients with NB and MYCN amplification in combination with 11qLOH might be less likely to respond to neoadjuvant chemotherapy when compared with patients with NB and MYCN amplification without 11qLOH.
Exogenous Cysteine Improves Mercury Uptake and Tolerance in Arabidopsis by Regulating the Expression of Heavy Metal Chelators and Antioxidative Enzymes
Authors: Kim Yeon-Ok , Gwon Yonghyun , Kim Jangho
Published: June 2022

Cysteine (Cys) is an essential amino acid component of the major heavy metal chelators, such as glutathione (GSH), metallothioneins (MTs), and phytochelatins (PCs), which are involved in the pathways of mercury (Hg) tolerance in plants. However, the mechanism through which Cys facilitates Hg tolerance in plants remains largely unclear. In this study, we investigated the effects of exogenous Cys on Hg uptake in the seedlings, roots, and shoots of Arabidopsis throughout 6 and 36 h of Hg exposure and on the regulation of Hg detoxification by heavy metal chelators and antioxidative enzymes. The results showed that exogenous Cys significantly improved Hg tolerance during the germination and seedling growth stages in Arabidopsis. Exogenous Cys significantly promoted Hg uptake in Arabidopsis roots by upregulating the expression of the Cys transporter gene AtLHT1, resulting in increased Hg accumulation in the roots and seedlings. In Arabidopsis seedlings, exogenous Cys further increased the Hg-induced glutathione synthase (GS1 and GS2) transcript levels, and the Hg and Hg + Cys treatments greatly upregulated MT3 expression after 36 h exposure. In the roots, MT3 was also significantly upregulated by treatment of 36 h of Hg or Hg + Cys. Notably, in the shoots, MT2a expression was rapidly induced (10-fold) in Hg presence and further markedly increased (20-fold) by exogenous Cys. Moreover, in the seedlings, exogenous Cys upregulated the transcripts of all superoxide dismutase (CuSOD1, CuSOD2, MnSOD1, FeSOD1, FeSOD2, and FeSOD3) within 6 h and subsequently increased the Hg-induced GR1 and GR2 transcript levels at 36 h, all of which could eliminate the promotion of reactive oxygen species production and cell damage caused by Hg. Additionally, exogenous Cys upregulated all the antioxidative genes rapidly in the roots and subsequently increased the expression of CuSOD1, CuSOD2, and MnSOD1 in the shoots. These results indicate that exogenous Cys regulates the transcript levels of heavy metal chelators and antioxidative enzymes differently in a time- and organ-specific manner under Hg stress. Taken together, our study elucidates the positive functional roles of exogenous Cys in the Hg uptake and tolerance mechanisms of Arabidopsis.
The Emotional Lockdown: How Social Distancing and Mask Wearing Influence Mood and Emotion Recognition in Adolescents and Adults
Authors: Kulke Louisa , Langer Theresia , Valuch Christian
Published: June 2022

During the COVID-19 pandemic, government-mandated protection measures such as contact restrictions and mask wearing significantly affected social interactions. In the current preregistered studies we hypothesized that such measures could influence self-reported mood in adults and in adolescents between 12 and 13 years of age, who are in a critical phase of social development. We found that mood was positively related to face-to-face but not to virtual interactions in adults and that virtual interactions were associated with negative mood in adolescents. This suggests that contact restrictions leading to a decrease in face-to-face compared to virtual interactions may be related to negative mood. To understand if prolonged exposure to people wearing masks during the pandemic might be related to increased sensitivity for subtle visual cues to others’ emotions from the eye region of the face, we also presented both age groups with the same standardized emotion recognition test. We found slightly better performance in emotion recognition from the eyes in our student sample tested during the pandemic relative to a comparable sample tested prior to the pandemic although these differences were restricted to female participants. Adolescents were also better at classifying emotions from the eyes in the current study than in a pre-pandemic sample, with no gender effects occurring in this age group. In conclusion, while social distancing might have detrimental effects on self-reported mood, the ability to recognize others’ emotions from subtle visual cues around the eye region remained comparable or might have even improved during the COVID-19 pandemic.
The Impact of Authentic Leadership on Innovative Work Behavior: Mediating Roles of Proactive Personality and Employee Engagement
Authors: Bai Yina , Wang Zheng , Alam Mehboob , Gul Fozia , Wang Yiqun
Published: June 2022

The purpose of the study is to investigate the impact of authentic leadership and proactive personality on innovative work behavior through dual mediation approach. This study applied a judgment sampling technique and data were gathered from 311 high-tech manufacturing industries of Shenzhen, China. The measurement model and structural model were tested using structural equation modeling technique through AMOS 24 software. The results indicate that authentic leadership has a positive and significant effect on proactive personality. Meanwhile, findings show that proactive personality has a significant impact on innovative work behavior. Moreover, findings show that proactive personality positively mediates the relationship between authentic leadership and innovative work behavior. Furthermore, findings illustrate that work engagement positively mediates the relationship between proactive personality and innovative work behavior. This study provides insightful and valuable implications to high-tech manufacturing industries executives and regulators interested in organizational productivity.
A Qualitative Study on Emotions Experienced at the Coast and Their Influence on Well-Being
Authors: Severin Marine I. , Raes Filip , Notebaert Evie , Lambrecht Luka , Everaert Gert , Buysse Ann
Published: June 2022

Coastal environments are increasingly shown to have a positive effect on our health and well-being. Various mechanisms have been suggested to explain this effect. However, so far little focus has been devoted to emotions that might be relevant in this context, especially for people who are directly or indirectly exposed to the coast on a daily basis. Our preregistered qualitative study explored how coastal residents experience the emotions they feel at the coast and how they interpret the effect these emotions have on them. We conducted semi-structured interviews with a purposive sample of eight Belgian coastal residents aged 21–25 years old. The interviews were analyzed with the approach of interpretative phenomenological analysis. Five superordinate themes were identified and indicate that, for our participants, the coast represents a safe haven (1) in which they can experience emotional restoration (2), awe (3), and nostalgia (4). These emotional states are accompanied with adaptive emotion regulating strategies (5), such as reflection and positive reappraisal, that may facilitate coping with difficult thoughts and feelings. Our study demonstrates the importance of investigating specific emotions and related processes triggered at the coast and how these could contribute to the therapeutic value of the coast.
Psychological Well-Being, Prenatal Attachment, and Quality of Early Mother-Infant Interaction: A Pilot Study With a Sample of Mothers With or Without Cancer History
Authors: Bozicevic Laura , Ponti Lucia , Smorti Martina , Pravettoni Gabriella , Peccatori Fedro Alessandro , Cassani Chiara , Nastasi Giuseppe , Sarchi Valentina , Bonassi Lucia
Published: June 2022

Given the positive impact of high-quality mother–infant interaction on child development, and that such relationship might be hindered by maternal stresses such past cancer, research is needed to understand protective and risk factors in this clinical population. As almost no data is available on the impact of history of cancer on the quality of mother–infant interaction, a multicentric and longitudinal pilot study was conducted. Differences in women’s prenatal psychological well-being and attachment (T1, third trimester), and postnatal quality of mother–infant interaction (T2, 2–5 months) were assessed in a sample of Italian mothers with (N = 11) or without cancer history (N = 13). Results showed that women did not differ significantly in their prenatal well-being (assessed with the Profile of Mood States questionnaire) and levels of attachment (assessed with the Prenatal Attachment Inventory). Looking at mother–infant interactions (assessed using the Global Rating Scale at T2), while maternal sensitivity, warmth and intrusiveness, and infant distress and attentiveness did not differ between the two groups, in the clinical group, mothers were more remote and less absorbed in the infant, and infants showed fewer positive communications. These findings might shed light on potential protective and risk factors for early parenting and later child outcomes in this clinical population.
Does Internet Connect to Social Justice Perception in China?
Authors: Zhou Dong , Zhu Jinyu , Guo Yihan
Published: June 2022

The world has witnessed an important and dramatic transition during the past decades, with social and economic challenges related to the advancement of digital technologies. Meanwhile, inequalities of distributions of resources and opportunities obstinately exist around the world. This study innovates by utilizing household survey datasets to empirically evaluate the impact of Internet utilization on individual’s perception toward social justice in China. Estimates suggest that Internet utilization generates a significant negative effect on perceived social justice (in general, −5%). In China, there are 1.032 billion Internet users by the end of 2021, accounting for 73% of the total population (China Internet Network Information Center). It suggests that 3.65% of the population, around 5 million people, might consider the current society is injustice if all circumstances remain unchanged. For robustness checks, we not only run multivariate regressions, implemented different model specification, and used alternative measures as well as datasets, but also approached instrumental variable estimation with regional rainfall for causal inference. Consistent conclusions are found. Also, we found stronger negative effects among male, eastern provinces, and urban area samples. To the end, our results shed lights on policy implication, for example, Internet associated public interventions can be focused on justice cultivation and information transparency.
Testing the Representational Deficit Hypothesis: From the Aspect of Chinese Learners’ Acquisition of Affixation ‘-s’ for Third Person Singular Verbs and Plural Nouns
Authors: Li Ni , Yang Lianrui
Published: June 2022

The persistent difficulty in making verbal inflections is commonly recognized for second language learners, especially for Chinese-speaking students. Researchers put forward different hypotheses to explain the problems in acquiring inflectional morphology. Among them, the representational deficit hypothesis deficit (RDH), advocated by Hawkins and Liszka, indicates that adult learners will fail to make inflectional morphology to interpret the corresponding syntactic feature if there is no counterpart system in their native language. In English, affix morpheme ‘-s’ marks either third person singular (3SG) in the present tense or regular plural nouns. In contrast, Chinese is a language which lacks 3SG markings but presents the morpheme ‘men’ to reflect a plural feature for nouns with a human property. To test the applicability of the RDH in the domain of affix ‘-s’ for English learners of Chinese, the present study observed the morphological inflections of the third person singular and plural ‘-s’ in 33 Chinese EFL learners’ written and spoken production tasks. The results show that the participants distinguished between the inflectional morphology in regular plural and 3SG thematic verb markings, which was compatible with the RDH. Additionally, other phenomena related to 3SG and plural morphological inflections provided strands of evidence for the RDH, for instance, L2 exposure age, a prominent overuse of plural ‘-s’, and exceptional cases for more 3SG ‘-s’ markings in the written data. Except for the account of morphosyntactic processes in the RDH, other factors, such as input frequency, difficulty of paradigm uniformity, and acquisition order, were referred to as the way that the L2 learners’ acquisition of the morphological inflections was affected.
Green Innovation Behavior Toward Sustainable Tourism Development: A Dual Mediation Model
Authors: Li XiaoJuan
Published: June 2022

The rapid increase in globalization has fostered the emerging ecological challenges to halt human civilization, substantially highlighting the need for environmental management. The study’s primary objective is to analyze the impact of environmental perception of corporate social responsibility (CSR) and attitude toward environmental CSR on sustainable tourism development while considering the mediating role of national park identification goals and employee pro-environmental behavior. The data was collected from the 338 employees working in the Chinese tourist firms’. The study variable’s reliability and validity was checked by using Composite Reliability (CR) and Average Variance Extracted (AVE). Study results show that environmental CSR perception and attitude toward environmental CSR positively impact sustainable tourism development, national park Goal identification, and employee pro-environmental behavior. National Park goal identification and employee pro-environmental behavior mediate between sustainable tourism development and environmental perception of corporate social responsibility and attitude toward environmental CSR.
Cerebral Cortical Activity During Academic Stress Amongst Undergraduate Medical Students at Kampala International University (Uganda)
Authors: Mujinya Regan , Kalange Muhamudu , Ochieng Juma John , Ninsiima Herbert Izo , Eze Ejike Daniel , Afodun Adam Moyosore , Nabirumbi Ritah , Sulaiman Sheu Oluwadare , Kairania Emmanuel , Echoru Isaac , Okpanachi Alfred Omachonu , Matama Kevin , Asiimwe Oscar Hilary , Nambuya Grace , Usman Ibe Michael , Obado Osuwat Lawrence , Zirintunda Gerald , Ssempijja Fred , Nansunga Miriam , Matovu Henry , Ayikobua Emmanuel Tiyo , Nganda Ponsiano Ernest , Onanyang David , Ekou Justine , Musinguzi Simon Peter , Ssimbwa Godfrey , Kasozi Keneth Iceland
Published: June 2022

Background

Stress among medical students is related to their academic lifespan; however, information on brain health among medical students from developing countries continues to be scarce. The objective of this study was to establish perceived academic stress levels, assess the ability to cope with stress, and investigate its effects on the visual reaction time (VRT), audio reaction time (ART), and tactile reaction time (TRT) in the somatosensory cortex among medical students of Uganda.

Methods

This was a cross-sectional study conducted among preclinical (n = 88) and clinical (n = 96) undergraduate medical students at Kampala International University Western Campus. A standard Perceived Stress Scale (PSS) was used to categorize stress into low, moderate, and severe while the ability to cope with stress was categorized into below average, average, above average, and superior stresscoper (SS). Data on reaction time were acquired through VRT, ART, and TRT using the catch-a-ruler experiment, and this was analyzed using SPSS version 20.

Results

This study shows that preclinical students are more stressed than clinical students (PSS prevalence for low stress = preclinical; clinical: 40, 60%). Moderate stress was 48.4 and 51.6% while high perceived stress was 75 and 25% among preclinical and clinical students. Among male and female students in preclinical years, higher TRT and VRT were found in clinical students showing that stress affects the tactile and visual cortical areas in the brain, although the VRT scores were only significantly (P = 0.0123) poor in male students than female students in biomedical sciences. Also, highly stressed individuals had higher TRT and ART and low VRT. SS had high VRT and ART and low TRT in preclinical students, demonstrating the importance of the visual cortex in stress plasticity. Multiple regression showed a close relationship between PSS, ability to cope with stress, age, and educational level (P < 0.05), demonstrating the importance of social and psychological support, especially in the biomedical sciences.

Conclusion

Preclinical students suffer more from stress and are poorer SS than clinical students. This strongly impairs their cortical regions in the brain, thus affecting their academic productivity.
Would Illness Representations of COVID-19 and COVID-19 Fears During Clinic Visits Promote or Reduce Behavioral Intention to Seek Medical Consultations for Flu Symptoms? A Random Telephone Survey in Hong Kong, China
Authors: Luo Sitong , She Rui , Lau Mason M. C. , Lau Joseph T. F.
Published: June 2022

Background

The study investigated the level of behavioral intention to consult doctors for flu symptoms (BICDFS) during the Coronavirus Disease 2019 (COVID-19) pandemic and examined its associations with illness representations of COVID-19 and fear of COVID-19 during clinic visits in a general Chinese adult population.

Methods

A random telephone survey was conducted among 300 residents in Hong Kong, China in April 2020 when the second wave of COVID-19 was just ended in the region. The participants were asked about their intention to consult doctors if they had mild or severe flu symptoms in the next week (from 1 = definitely no to 5 = definitely yes). Illness representations of COVID-19 were measured by the Chinese version of the Brief Illness Perception Questionnaire (BIPQ). The fear of COVID-19 during clinic visits were assessed by two single items. Linear regression adjusted for background variables and hierarchical strategies were employed.

Results

Of the participants, 52.3 and 92.0% showed an intention to consult doctors for mild and severe flu symptoms, respectively. Adjusted for background factors, COVID-19-related cognitive representations (consequences: standardized b = 0.15, p = 0.010; understanding: standardized b = 0.21, p = 0.001) and emotional representations (concern: standardized b = 0.17, p = 0.001; negative emotions: standardized b = 0.19, p = 0.001) were positively associated with BICDFS. In the hierarchical model, independent significant factors of BICDFS included understanding (standardized b = 0.16, p = 0.013) and negative emotions (standardized b = 0.17, p = 0.008). The fear-related variables showed non-significant associations with the BICDFS.

Conclusions

Promotion of care-seeking behaviors for flu symptoms during the COVID-19 pandemic should consider improving people's understanding of COVID-19 and providing advice on related coping strategies for emotional responses to COVID-19.
An Integrated Multi-Risk Assessment for Floods and Drought in the Marrakech-Safi Region (Morocco)
Authors: Cotti Davide , Harb Mostapha , Hadri Abdessamad , Aboufirass Mohammed , Chaham Khalid Rkha , Libertino Andrea , Campo Lorenzo , Trasforini Eva , Krätzschmar Elke , Bellert Felicitas , Hagenlocher Michael
Published: June 2022

Multi-risk assessments are being increasingly proposed as a tool to effectively support policy-makers in reducing impacts from natural hazards. The complexity of multi-risk requires assessment approaches capable of capturing multiple components of risk (e.g., different hazards, exposed elements, and dimensions of vulnerability) in a coherent frame of reference, while at the same time providing an intuitive entry point to allow participation of relevant stakeholders. Contributing to the emerging multi-risk literature, we carried out a multi-risk assessment for the Marrakech-Safi region (Morocco)—an important economic and demographic hub in the country that is prone to multiple natural hazards, most notably floods and droughts. Through multiple consultations with local experts and stakeholders, a multi-risk assessment framework was constructed based on a set of single-risks related to flood and drought hazards. For each risk, spatial analysis was employed to assess the hazard exposure component of multi-risk, while a set of vulnerability indicators and stakeholder-informed weights were used to construct a composite indicator of vulnerability at the municipal level. For each municipality, the set of indicators and weights contributing to the composite indicator was designed to be dependent on the combination of risks the municipality is actually confronted with. The two components were aggregated using a risk matrix approach. Results show a significant proportion of municipalities (28%) reaching very high multi-risk levels, with a large influence of drought-related risks, and a prominent contribution of the vulnerability component on the overall multi-risk results. While the approach has allowed the exploration of the spatial variability of multi-risk in its multiple sub-components and the incorporation of stakeholders' opinions at different levels, more research is needed to explore how best to disentangle the complexity of the final multi-risk product into a tool capable of informing policy-makers in the identification of entry points for effective disaster risk governance.
The Making and Breaking of Social Ties During the Pandemic. Socio-Economic Position, Demographic Characteristics, and Changes in Social Networks
Authors: Bertogg Ariane , Koos Sebastian
Published: June 2022

Contact restrictions and distancing measures are among the most effective non-pharmaceutical measures to stop the spread of the SARS-CoV2 virus. Yet, research has only begun to understand the wider social consequences of these interventions. This study investigates how individuals' social networks have changed since the outbreak of the pandemic and how this is related to individuals' socio-economic positions and their socio-demographic characteristics. Based on a large quota sample of the German adult population, we investigate the loss and gain of strong and weak social ties during the pandemic. While about one third of respondents reported losing of contact with acquaintances, every fourth person has lost contact to a friend. Forming new social ties occurs less frequently. Only 10–15% report having made new acquaintances (15%) or friends (10%) during the pandemic. Overall, more than half of our respondents did not report any change, however. Changes in social networks are linked to both socio-demographic and socio-economic characteristics, such as age, gender, education, and migration background, providing key insights into a yet underexplored dimension of pandemic-related social inequality.