bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical and Experimental Analysis of the Load-Carrying Capacity of a Timber Semi-Rigid Dowel-Type Connection
Authors: Johanides Marek , Lokaj Antonin , Dobes Pavel , Mikolasek David
Published: October 2022

The paper deals with the analysis of the load-carrying capacity of a timber semi-rigid connection created from a system of two stands and a rung. The connection was made from glued laminated timber with metal mechanical dowel-type fasteners. Not only a common combination of bolts and dowels, but also fully threaded screws were used for the connection. The aim of the research and its motivation was to replace these commonly used fasteners with more modern ones, to shorten and simplify the assembly time, and to improve the load-carrying capacity of this type of connection. Each of these two types of connections was loaded statically, with a slow increase in force until failure. The paper presents results of the experimental testing. Three specimens were made and tested for each type of the connection. Experimental results were subsequently compared with numerical models. The achieved results were also compared with the assumption according to the currently valid standard. The results indicate that a connection using fully threaded screws provides a better load-carrying capacity.
Correction: Liu et al. 3D-Printed Double-Helical Biodegradable Iron Suture Anchor: A Rabbit Rotator Cuff Tear Model Materials 2022, 15, 2801
Authors: Liu Wen-Chih , Chang Chih-Hau , Chen Chung-Hwan , Lu Chun-Kuan , Ma Chun-Hsien , Huang Shin-I , Fan Wei-Lun , Shen Hsin-Hsin , Tsai Pei-I , Yang Kuo-Yi , Fu Yin-Chih
Published: October 2022

In the original publication [...]
Glass-Forming Ability and Corrosion Behavior of Ti-Based Amorphous Alloy Ti-Zr-Si-Fe
Authors: Bai Ling , Ding Ziyang , Zhang Haiying , Cui Chunxiang
Published: October 2022

Ti-based alloy Ti75Zr11Si9Fe5 (At %) and Ti66Zr11Si15Fe5Mo3 (At %) ribbons are fabricated by a single roller spun-melt technique, according to the three empirical rules. Both alloys are found to have a large, supercooled liquid region (ΔTx) before crystallization that reaches 80–90 K. The results show that both alloys possess excellent glass-forming abilities. The electrochemical measurement proves both amorphous alloys possess relatively high corrosion resistance in 3 mass% NaCl solution.
Studies on the Removal of Congo Red Dye by an Adsorbent Based on Fly-Ash@Fe3O4 Mixture
Authors: Harja Maria , Lupu Nicoleta , Chiriac Horia , Herea Dumitru-Daniel , Buema Gabriela
Published: October 2022

The effectiveness of a Fe3O4-loaded fly ash composite for the adsorption of Congo red dye was assessed in this work. The structure and properties of the magnetic adsorbent were established by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), and dynamic light scattering (DLS). The magnetic results showed a saturation magnetization value of 6.51 emu/g and superparamagnetic behavior. The main parameters that influence the removal of Congo red dye adsorbent such as dose, initial concentration, and contact time were examined. The Freundlich adsorption isotherm and pseudo-second-order kinetic model provided the best fit for the experimental findings. The Congo red dye’s maximum adsorption capacity of 154 mg/g was reported in the concentration range of 10–100 mg/L, using the proposed magnetic adsorbent. The results of the recyclability investigation demonstrated that the circular economy idea is valid. The adsorbent that was synthesized was also further characterized by XRD and FTIR techniques after Congo red dye adsorption.
Indocyanine Green-Containing Magnetic Liposomes for Constant Magnetic Field-Guided Targeted Delivery and Theranostics
Authors: Korolev Dmitry V. , Shulmeyster Galina A. , Istomina Maria S. , Nikiforov Alexey I. , Aleksandrov Ilia V. , Semenov Valentin G. , Galagudza Michael M.
Published: October 2022

The aim of the present study was to develop magnetic liposomes (MLPSs) incorporating an agent with the ability to act both as a photosensitizer and as a fluorophore for optical imaging. We therefore aimed to develop a preparation method for indocyanine green (ICG)-containing MLPS, followed by a detailed characterization of their physicochemical and magnetic properties. The ability of intravenously administered ICG-containing MLPSs to accumulate in tissue exposed to a constant magnetic field was tested in vivo. Using the thin film hydration method, 170-nm aqueous liposomes containing magnetic nanoparticles and indocyanine green were synthesized, followed by a detailed characterization of their physicochemical properties. It was shown that ICG-containing MLPSs possess the properties of T2 contrast for MRI. Apart from this, ICG-containing MLPSs were clearly visualized using near infrared fluorescent imaging, which was demonstrated in in vivo experiments showing an accumulation of ICG-containing MLPSs in the zone of magnetic field distribution produced by a previously implanted constant magnet in the tissue. Although not directly tested in the present study, therapeutic applications of ICG-containing MLPSs include magnetic hyperthermia, as well as the photodynamic, photothermal, and photoacoustic effects of ICG. Taking into account the fact that liposomes, iron oxide nanoparticles, and ICG are all FDA-approved agents, it is highly likely that ICG-containing MLPSs could be successfully translated to clinical practice.
A Review of the Self-Powered Wiegand Sensor and Its Applications
Authors: Lin Chiao-Chi , Tseng Yuan-Chieh , Chin Tsung-Shune
Published: October 2022

Self-powered magnetic sensors are fundamental for the development of Industry 4.0, the Internet of things (IoT), wireless sensor networks, unmanned vehicles, smart cities, and sustainability. This review aimed to elucidate the working principles, materials, manufacture, output properties, and perspectives of Wiegand sensors. A Wiegand sensor is composed of a magnetic sensing wire, which is called a Wiegand wire, and a pick-up coil for the output of an electrical signal and energy. The Wiegand sensor requires an external magnetic field of about 70 Gauss to induce Wiegand wire flux changes, which, in turn, generate an output pulse in the pick-up coil. Output energy of more than 3000 nJ per single pulse (open circuit) can be harvested. The output pulse is derived from the large Barkhausen effect. Therefore, the behavior of the sensor output is independent of the triggering and sensing frequencies. The objective of this review article was to comprehensively highlight research endeavors devoted to Wiegand sensors. Furthermore, application scenarios of current research results are highlighted to find potential gaps in the literature and future contributions. Perspectives and research opportunities of Wiegand sensors are proposed.
New Ceramides and Cerebrosides from the Deep-Sea Far Eastern Starfish Ceramaster patagonicus
Authors: Malyarenko Timofey V. , Zakharenko Viktor M. , Kicha Alla A. , Kuzmich Alexandra S. , Malyarenko Olesya S. , Kalinovsky Anatoly I. , Popov Roman S. , Svetashev Vasily I. , Ivanchina Natalia V.
Published: October 2022

Three new ceramides (1–3) and three new cerebrosides (4, 8, and 9), along with three previously known cerebrosides (ophidiocerebrosides C (5), D (6), and CE-3-2 (7)), were isolated from a deep-sea starfish species, the orange cookie starfish Ceramaster patagonicus. The structures of 1−4, 8, and 9 were determined by the NMR and ESIMS techniques and also through chemical transformations. Ceramides 1–3 contain iso-C21 or C23 Δ9-phytosphingosine as a long-chain base and have C16 or C17 (2R)-2-hydroxy-fatty acids of the normal type. Cerebroside 4 contains C22 Δ9-sphingosine anteiso-type as a long-chain base and (2R)-2-hydroxyheptadecanoic acid of the normal type, while compounds 8 and 9 contain saturated C-17 phytosphingosine anteiso-type as a long-chain base and differ from each other in the length of the polymethylene chain of (2R)-2-hydroxy-fatty acids of the normal type: C23 in 8 and C24 in 9. All the new cerebrosides (4, 8, and 9) have β-D-glucopyranose as a monosaccharide residue. The composition of neutral sphingolipids from C. patagonicus was described for the first time. The investigated compounds 1–3, 5–7, and 9 exhibit slight to moderate cytotoxic activity against human cancer cells (HT-29, SK-MEL-28, and MDA-MB-231) and normal embryonic kidney cells HEK293. Compounds 2, 5, and 6 at a concentration of 20 µM inhibit colony formation of MDA-MB-231 cells by 68%, 54%, and 68%, respectively. The colony-inhibiting activity of compounds 2, 5, and 6 is comparable to the effect of doxorubicin, which reduces the number of colonies by 70% at the same concentration.
Human Papillomavirus and Risk of Colorectal Cancer: An Analysis of Nationwide Claims Data
Authors: Hsu Chih-Hsiung , Lin Yu-Jyun , Chen Yong-Chen , Liu I-Lan , You San-Lin , Hu Je-Ming , Lin Tzu-Chiao , Chang Pi-Kai , Chen Chao-Yang , Chou Yu-Ching , Sun Chien-An
Published: October 2022

Background and Objectives: Although human papillomavirus (HPV) is a major etiology of cervical and anogenital cancers, whether it is associated with colorectal carcinogenesis is yet undetermined. Materials and Methods: The longitudinal association of HPV infection with colorectal cancer (CRC) was evaluated using 2000–2013 data from a nationwide Taiwanese claims database. In this retrospective cohort study, 358 patients with primary HPV diagnoses (HPV-infected cohort) and 1432 patients without such a diagnosis (HPV-uninfected cohort) were recruited between 2000 and 2006. Both cohorts were followed up to identify CRC incidences from 2006 to 2013. Hazard ratios (HRs) and their 95% confidence intervals (CIs) derived from Cox proportional hazards models were used to estimate the association between HPV and CRC risk. Results: The HPV-infected cohort had a significantly higher cumulative incidence of CRC than the HPV-uninfected cohort. The presence of HPV was associated with an increased risk of CRC (adjusted HR, 1.63; 95% CI, 1.02–3.62). Furthermore, the significant HPV–CRC risk association was evident in both sexes. Conclusions: This population-based cohort study reveals longitudinal evidence that HPV is associated with an increased risk of CRC. Further studies are required to verify the role of HPV in colorectal carcinogenesis.
miRNA Expression Associated with HbF in Saudi Sickle Cell Anemia
Authors: Cyrus Cyril , Vatte Chittibabu , Al-Nafie Awatif , Chathoth Shahanas , Akhtar Mohammed S. , Darwish Mohammed , Almohazey Dana , AlDubayan Saud H. , Steinberg Martin H. , Al-Ali Amein
Published: October 2022

Background and Objectives: Sickle cell anemia (SCA) is a hereditary monogenic disease due to a single β-globin gene mutation that codes for the production of sickle hemoglobin. Its phenotype is modulated by fetal hemoglobin (HbF), a product of γ-globin genes. Exploring the molecules that regulate γ-globin genes at both transcriptional and translational levels, including microRNA (miRNA), might help identify alternative therapeutic targets. Materials and Methods: Using next-generation sequencing we identified pre-miRNAs and mature miRNA expression signatures associated with different HbF levels in patients homozygous for the sickle hemoglobin gene. The involvement of identified miRNAs in potential SCD-related pathways was investigated with the DIANA TOOL and miRWalk 2.0 database. Results: miR-184 were most highly upregulated in reticulocytes. miR-3609 and miR-483-5p were most highly downregulated in sickle cell anemia with high HbF. miR-370-3p that regulates LIN28A, and miR-451a which is effective in modulating α- and β- globin levels were also significantly upregulated. miRNA targeted gene pathway interaction identified BCL7A, BCL2L1, LIN28A, KLF6, GATA6, solute carrier family genes and ZNF genes associated with erythropoiesis, cell cycle regulation, glycosphingolipid biosynthesis, cAMP, cGMP-PKG, mTOR, MAPK and PI3K-AKT signaling pathways and cancer pathways. Conclusions: miRNA signatures and their target genes identified novel miRNAs that could regulate fetal hemoglobin production and might be exploited therapeutically.
Effect of Graphene Oxide on the Properties of Polymer Inclusion Membranes for Gold Extraction from Acidic Solution
Authors: Husna Siti Madiha , Yusoff Abdul Hafidz , Mohan Mythili , Azmi Nur Aina , Ter Teo Pao , Shoparwe Noor Fazliani , Sulaiman Ahmad Ziad
Published: October 2022

The cyanidation leaching method is hazardous to the environment, but it is widely applied in the gold mining process because it is effective for gold extraction. This study fabricates polymer inclusion membranes (PIMs), which have environment-friendly properties, with graphene oxide (GO) as an alternative to the cyanidation leaching method for gold extraction. Poly(vinylidenefluoride-co-hexa-fluoropropylene)-based PIMs with different GO concentrations in five membranes (i.e., M1 (0 wt.%), M2 (0.5 wt.%), M3 (1.0 wt.%), M4 (1.5 wt.%), and M5 (2.0 wt.%)) are studied for their potential to extract gold from a hydrochloric acid solution. The membranes are prepared using di-(2-ethylhexyl) phosphoric acid as the extractant and dioctyl phthalate as the plasticizer. Scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis, ion exchange capacity, and water uptake are used to characterize the physical and chemical properties of the fabricated PIMs. The results show that the optimized membrane for gold extraction is M4 (1.5 wt.% GO), which yields a better performance on thermal stability, ion exchange capacity (IEC), and water uptake. M4 (1.5 wt.% GO) also exhibits a smooth and dense structure, with the maximum extraction efficiency obtained at 84.71% of extracted gold. In conclusion, PIMs can be used as an alternative for extracting gold with a better performance by the presence of 1.5 wt.% GO in membrane composition.
Identifying Characteristic Frequencies in the Electrochemical Impedance of Ion-Exchange Membrane Systems
Authors: Moya Antonio Angel
Published: October 2022

In this study, the characteristic frequencies of the electrochemical impedance of ion-exchange membrane systems constituted by the membrane and two diffusion boundary layers adjacent to the membrane were investigated. Approximations of the impedance of the Randles equivalent electric circuit in multiple frequency ranges were considered, and the characteristic frequencies of the zeros and poles of orders ½ and 1 were derived. The characteristic geometric frequencies, those associated with the interfacial charge transfer and the diffusive transport processes, as well as those associated with the transitions between processes, were identified by means of analytical expressions.
Investigation of Phosphatidylserine-Transporting Activity of Human TMEM16C Isoforms
Authors: Kim Hanggu , Kim Eunyoung , Lee Byoung-Cheol
Published: October 2022

Lipid scrambling is a rapid process that dissipates the asymmetrical distribution of phospholipids in the plasma membrane. It is involved in various physiological functions such as blood coagulation and apoptosis. Many TMEM16 members are recognized as Ca2+-activated phospholipid scramblases, which transport phospholipids between the two leaflets of the plasma membrane nonspecifically and bidirectionally; among these, TMEM16C is abundant in the brain, especially in neuronal cells. We investigated the scrambling activity of three human TMEM16C isoforms with different N-terminus lengths. After optimizing conditions to minimize endogenous scrambling activity, an annexin V-based imaging assay was used to detect phosphatidylserine (PS) scrambling in 293T cells. Unlike previous results, our data showed that human TMEM16C isoform 1 and isoform 3 exposed PS to the cell surface. A surface biotinylation assay showed that the surface expression of isoform 2, which did not show scrambling activity, was ~5 times lower than the other isoforms. In contrast to other TMEM16 proteins, flux assays and electrophysiology recording showed TMEM16C does not possess ion-transporting activity. We conclude that the N-terminus of TMEM16C determines whether TMEM16C can translocate to the plasma membrane and facilitate scrambling activity; membrane-localized TMEM16C isoforms 1 and 3 transport PS to the outer leaflet.
Effect of α Phase on Dynamic Mechanical Properties and Failure of Ti-4Al-5Mo-5V-5Cr-1Nb Alloy after Two-Stage Aging
Authors: Wang Tao , Liu Xianghong , Feng Yong , Wang Kaixuan , Sun Shangqing , Yang Dong , Sun Feng
Published: October 2022

This work studied the high strain rate behavior of the Ti-4Al-5Mo-5V-5Cr-1Nb (Ti-45551) alloy after two-stage aging, with dynamic tension tests conducted using the split Hopkinson tensile bar. The results show that the microstructure, especially the size and distribution of the α phase, significantly affects the mechanical and failure behaviors of the Ti-45551 alloy under dynamic loading. As the strain rate increases, increasing trends can be observed in both ductility and strength, which is intimately related to the activation of the dislocation slip in the α phase. Moreover, obvious strain softening was found in the Ti-45551 alloy under dynamic loading. In this study, the microstructure observations suggest that dislocation slips are highly active in the α phase under dynamic loading. Fractographic characterization of the fracture surfaces under dynamic loading reveals a uniform distribution of ductile dimples, which indicates that a uniform distribution of the nano-scale α phase can effectively reduce brittle fracture tendency. Our studies provide a comprehensive picture of how strain rate drives dynamic plasticity and failure mode in the Ti alloy.
An Experimental Study of the Surface Roughness of SiCp/Al with Ultrasonic Vibration-Assisted Grinding
Authors: Ying Jie , Yin Zhen , Zhang Peng , Zhou Peixiang , Zhang Kun , Liu Zihao
Published: October 2022

Due to the differences in mechanical properties of Al and SiC particles, the problems of SiC particle pullout and high surface roughness will occur in the processing of SiCp/Al composites. However, the ultrasonic vibration-assisted grinding of SiCp/Al can effectively decrease the appearance of such problems. A comparative experimental study of the ultrasonic vibration-assisted and ordinary grinding of SiCp/Al is conducted. First, the effect of ultrasonic amplitude on the removal form of SiC is summarized by observing the surface morphology of the sample. Then, the primary reasons for the pullout of SiC particles and high surface roughness in SiCp/Al processing are analyzed. The variation law of the surface roughness of SiCp/Al under different ultrasonic amplitudes and grinding parameters is summarized through a single-factor experiment. The results show that ultrasonic vibration-assisted grinding is beneficial for reducing the surface roughness of SiCp/Al. When grinding linear speed of grinding wheel vs increases from 2.512 m/s to 7.536 m/s, surface roughness Ra decreases from 0.25 µm to 0.16 µm. when feed rate vw increases from 100 mm/min to 1700 mm/min, surface roughness Ra increases from 0.13 µm to 0.20 µm. When grinding depth ap increases from 0.01 mm to 0.05 mm, surface roughness Ra increases from 0.13 µm to 0.19 µm. When ultrasonic amplitude A is increased from 0 µm to 2 µm, surface roughness Ra decreases from 0.26 µm to 0.15 µm. When ultrasonic amplitude A is increased from 2 µm to 4.4 µm, surface roughness Ra increases from 0.15 µm to 0.18 µm.
Tribological, Corrosion, and Mechanical Properties of Selective Laser Melted Steel
Authors: Ralls Alessandro M. , John Merbin , Noud Jennifer , Lopez Jose , LeSourd Kasey , Napier Ian , Hallas Nicholas , Menezes Pradeep L.
Published: October 2022

In additive manufacturing (AM), selective laser melting (SLM) is a relatively novel technique that utilizes thermal energy via laser beams to melt and solidify metallic powders into three-dimensional components. Compared to traditional manufacturing techniques, SLM is advantageous because it is more time-efficient, cost-effective, and allows for the fabrication of components with superior mechanical, tribological, and corrosion performances. However, much of the existing literature highlights the influence of SLM on softer materials such as aluminum or magnesium due to their thermal expansion coefficients rather than on materials such as steel. This review aims to encapsulate the existing literature on SLM steel and understand the factors that allow for its fabrication and the underlying mechanisms that dictate its mechanical, tribological, and corrosion performance. By understanding the trends of laser energy density (LED), scanning patterns, and building directions for these properties, a comprehensive understanding of SLM steel can be achieved. Additionally, through this understanding, the future directions of this research and suggestions will be provided to continue progressing the field in an impactful direction.
Electrochemical Behaviour and Direct Cell Viability Analysis of Hybrid Implants Made of Ti-6Al-4V Lattices Infiltrated with a Bioabsorbable Zn-Based Alloy
Authors: Gabay Bass Noa , Katarivas Levy Galit , Ron Tomer , Vago Razi , Goldman Jeremy , Shirizly Amnon , Aghion Eli
Published: October 2022

Biodegradable metals are being developed for biomedical implants or components of implants. Biodegradable zinc-based materials, in particular, have been shown to promote bone regeneration in orthopaedic applications. Here, we investigated the potential of a hybrid Ti-Zn system, comprising a Ti-6Al-4V biostable lattice produced by additive manufacturing (AM) infiltrated by a bioabsorbable Zn-2%Fe alloy, to serve as an osseointegrated implant for dental and orthopaedic applications. The osseointegration of implants can be enhanced by a porous implant structure that facilitates bone ingrowth to achieve superior bonding between the bone tissue and the implant. The hybrid material was evaluated in terms of microstructure and localized chemical composition using scanning and transmission electron microscopy with special attention to the interface between the Ti-based lattice and the biodegradable alloy. The electrochemical behaviour of the Ti-Zn system was analysed in a simulated physiological environment in terms of open circuit potential test and cyclic potentiodynamic polarization. Cytotoxicity was evaluated using direct cell viability tests. The results demonstrate desirable properties of the hybrid Ti-Zn system as a non-cytotoxic material with an acceptable corrosion rate.
Effects of Acute Moderate Hypoxia versus Normoxia on Metabolic and Cardiac Function and Skeletal Muscle Oxygenation during Endurance Exercise at the Same Heart Rate Level
Authors: Park Hun-Young , Jung Won-Sang , Kim Sung-Woo , Seo Jisu , Sun Yerin , Choi Jae-Ho , Kim Jisu , Lim Kiwon
Published: October 2022

This study aimed to investigate the effects of acute moderate hypoxia (HYP), compared with those of normoxia (NORM), during endurance exercise with the same HR level on metabolic function, skeletal muscle oxygenation, and cardiac function. Twelve healthy men (aged 25.1 ± 2.3 years) completed 30 min of endurance exercise using a cycle ergometer with the same HR level (136.5 ± 1.5 bpm) corresponding to 70% maximal heart rate (HRmax) under NORM (760 mmHg) and HYP (526 mmHg, simulated 3000 m altitude) after a 30 min exposure in the respective environments on different days, in random order. Exercise load, rating of perceived exertion (RPE), metabolic function (saturation of percutaneous oxygen; SpO2, minute ventilation; oxygen uptake; VO2, carbon dioxide excretion; respiratory exchange ratio; RER, and oxygen pulse), skeletal muscle oxygen profiles (oxyhemoglobin, oxhb, deoxyhemoglobin, dxhb, total hemoglobin, and tissue oxygenation index; StO2), and cardiac function (heart rate, stroke volume, cardiac output, end-diastolic volume, end-systolic volume, and ejection fraction) were measured during endurance exercise. HYP showed a lower exercise load with the same RPE during exercise than did NORM. In addition, HYP showed a lower SpO2, VO2, oxygen pulse, oxhb, and StO2, and a higher RER and dxhb during exercise than NORM. We found that HYP showed lower exercise load and VO2 at the same RPE than NORM and also confirmed a higher anaerobic metabolism and oxygen inflow into skeletal muscle tissue due to the limitation of oxygen delivery capacity.
Gamma-ray Irradiation of Rodent Diets Alters the Urinary Metabolome in Rats with Chemically Induced Mammary Cancer
Authors: Prasain Jeevan K. , Wilson Landon S. , Grubbs Clinton , Barnes Stephen
Published: October 2022

In this study, a comparative, untargeted metabolomics approach was applied to compare urinary metabolite profiles of rats fed irradiated and non-irradiated diets. γ-Irradiated and non-irradiated NIH 7001 diet was given orally to animals beginning 5 days after exposure to the carcinogen N-methyl-N-nitrosourea and continued for 120 days. There was a 36% reduction in mammary tumor incidence in rats consuming the γ-irradiated diet, compared to rats receiving the non-irradiated form of the same diet. Urine samples from rats fed with γ-irradiated and non-irradiated diets were analyzed using nanoLC-MS/MS on a Q-TOF mass spectrometer, collecting positive and negative ion data. Data processing involved feature detection and alignment with MS-DIAL, normalization, mean-centering and Pareto scaling, and univariate and multivariate statistical analysis using MetaboAnalyst, and pathway analysis with Mummichog. Unsupervised Principal Component Analysis and supervised Partial Least Squares-Discriminant Analysis of both negative and positive ions revealed separation of the two groups. The top 25 metabolites from variable importance in projection scores >1 showed their contributions in discriminating urines the γ-irradiated diet fed group from non-irradiated control diet group. Consumption of the γ-irradiated diet led to alteration of several gut microbial metabolites such as phenylacetylglycine, indoxyl sulfate, kynurenic acid, hippurate and betaine in the urine. This study provides insights into metabolic changes in rat urine in response to a γ-irradiated diet which may be associated with mammary cancer prevention.
Integrated Lipidomic and Metabolomics Analysis Revealing the Effects of Frozen Storage Duration on Pork Lipids
Authors: Feng Xiaohui , Li Jing , Zhang Longchao , Rao Zhenghua , Feng Shengnan , Wang Yujiao , Liu Hai , Meng Qingshi
Published: October 2022

Frozen storage is an important strategy to maintain meat quality for long-term storage and transportation. Lipid oxidation is one of the predominant causes of the deterioration of meat quality during frozen storage. Untargeted lipidomic and targeted metabolomics were employed to comprehensively evaluate the effect of frozen duration on pork lipid profiles and lipid oxidative products including free fatty acids and fatty aldehydes. A total of 688 lipids, 40 fatty acids and 14 aldehydes were successfully screened in a pork sample. We found that ether-linked glycerophospholipids, the predominant type of lipids, gradually decreased during frozen storage. Of these ether-linked glycerophospholipids, ether-linked phosphatidylethanolamine and phosphatidylcholine containing more than one unsaturated bond were greatly influenced by frozen storage, resulting in an increase in free polyunsaturated fatty acids and fatty aldehydes. Among these lipid oxidative products, decanal, cis-11,14-eicosenoic acid and cis-5,8,11,14,17-dicosapentaenoic acid can be considered as potential indicators to calculate the freezing time of unknown frozen pork samples. Moreover, over the three-month frozen storage, the first month was a rapid oxidation stage while the other two months were a slow oxidation stage.
Metabolomic Study of Urine from Workers Exposed to Low Concentrations of Benzene by UHPLC-ESI-QToF-MS Reveals Potential Biomarkers Associated with Oxidative Stress and Genotoxicity
Authors: Mendes Michele P. R. , Paiva Maria José N. , Costa-Amaral Isabele C. , Carvalho Leandro V. B. , Figueiredo Victor O. , Gonçalves Eline S. , Larentis Ariane L. , André Leiliane C.
Published: October 2022

Benzene is a human carcinogen whose exposure to concentrations below 1 ppm (3.19 mg·m−3) is associated with myelotoxic effects. The determination of biomarkers such as trans-trans muconic acid (AttM) and S-phenylmercapturic acid (SPMA) show exposure without reflecting the toxic effects of benzene. For this reason, in this study, the urinary metabolome of individuals exposed to low concentrations of benzene was investigated, with the aim of understanding the biological response to exposure to this xenobiotic and identifying metabolites correlated with the toxic effects induced by it. Ultra-efficient liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer (UHPLC-ESI-Q-ToF-MS) was used to identify metabolites in the urine of environmentally (n = 28) and occupationally exposed (n = 32) to benzene (mean of 22.1 μg·m−3 and 31.8 μg·m−3, respectively). Non-targeted metabolomics analysis by PLS-DA revealed nine urinary metabolites discriminating between groups and statistically correlated with oxidative damage (MDA, thiol) and genetic material (chromosomal aberrations) induced by the hydrocarbon. The analysis of metabolic pathways revealed important alterations in lipid metabolism. These results point to the involvement of alterations in lipid metabolism in the mechanisms of cytotoxic and genotoxic action of benzene. Furthermore, this study proves the potential of metabolomics to provide relevant information to understand the biological response to exposure to xenobiotics and identify early effect biomarkers.
Autonomous Binarized Focal Loss Enhanced Model Compression Design Using Tensor Train Decomposition
Authors: Liu Mingshuo , Luo Shiyi , Han Kevin , DeMara Ronald F. , Bai Yu
Published: October 2022

Deep learning methods have exhibited the great capacity to process object detection tasks, offering a practical and viable approach in many applications. When researchers have advanced deep learning models to improve their performance, the model derived from the algorithmic improvement may itself require complementary increases in computational and power demands. Recently, model compression and pruning techniques have received more attention to promote the wide employment of the DNN model. Although these techniques have achieved a remarkable performance, the class imbalance issue during the mode compression process does not vanish. This paper exploits the Autonomous Binarized Focal Loss Enhanced Model Compression (ABFLMC) model to address the issue. Additionally, our proposed ABFLMC can automatically receive the dynamic difficulty term during the training process to improve performance and reduce complexity. A novel hardware architecture is proposed to accelerate inference. Our experimental results show that the ABFLMC can achieve higher accuracy, faster speed, and smaller model size.
A Vertical Single Transistor Neuron with Core–Shell Dual-Gate for Excitatory–Inhibitory Function and Tunable Firing Threshold Voltage
Authors: Lee Taegoon , Jeon Seung-Bae , Kim Daewon
Published: October 2022

A novel inhibitable and firing threshold voltage tunable vertical nanowire (NW) single transistor neuron device with core–shell dual-gate (CSDG) was realized and verified by TCAD simulation. The CSDG NW neuron is enclosed by an independently accessed shell gate and core gate to serve an excitatory–inhibitory transition and a firing threshold voltage adjustment, respectively. By utilizing the shell gate, the firing of specific neuron can be inhibited for winner-takes-all learning. It was confirmed that the independently accessed core gate can be used for adjustment of the firing threshold voltage to compensate random conductance variation before the learning and to fix inference error caused by unwanted synapse conductance change after the learning. This threshold voltage tuning can also be utilized for homeostatic function during the learning process. Furthermore, a myelination function which controls the transmission rate was obtained based on the inherent asymmetry between the source and drain in vertical NW structure. Finally, using the CSDG NW neuron device, a letter recognition test was conducted by SPICE simulation for a system-level validation. This multi-functional neuron device can contribute to construct a high-density monolithic SNN hardware combining with the previously developed vertical synapse MOSFET devices.
Pressure Relief and Bolt Grouting Reinforcement and Width Optimization of Narrow Coal Pillar for Goaf-Side Entry Driving in Deep Thick Coal Seam: A Case Study
Authors: Li Liangshan , Qian Deyu , Yang Xingguo , Jiao Hexi
Published: October 2022

Goaf-side roadway driving with narrow coal pillars could obviously improve coal resource recovery rates compared with traditional large, wide pillars, and this is pivotal to the sustainable development of underground mines. However, it is very difficult to control the stability of goaf-side roadway driving, especially in deep, thick coal seams with large and high working faces. In order to control the stability of goaf-side entry driving in working face 210106 of the deep and thick coal seam in Xinji No. 2 Coal Mine in Anhui Province of China, we carried out field investigations, theoretical calculations, numerical simulations, and an engineering practice to identify the main factors influencing the deformation of the surrounding rock in order to optimize the width of the narrow coal pillar and to propose countermeasures for goaf-side entry driving. Our results show that the main factors influencing deformation of the rock surrounding the roadways at working face 210106 in Xinji No. 2 Coal Mine include high ground stress, large mining height, thick sandstone in the roof, and the residual abutment pressure of the adjacent goaf. The results obtained from theoretical calculations, the numerical simulations, and the engineering practice indicate that a 5 m-wide coal pillar is relatively appropriate and feasible. The countermeasures of pressure relief by blasting roof cutting and bolt grouting reinforcement were carried out to control the stability for goaf-side entry driving. Field measurements indicated that deformations of goaf-side entry driving in deep, thick coal seams could be efficiently controlled. The maximum deformations of sidewall-to-sidewall and roof-to-floor were 100 mm and 350 mm, respectively.
In Situ Determination of Radioactivity Levels and Radiological Doses in and around the Gold Mine Tailing Dams, Gauteng Province, South Africa
Authors: Moshupya Paballo M. , Mohuba Seeke C. , Abiye Tamiru A. , Korir Ian , Nhleko Sifiso , Mkhosi Margaret
Published: October 2022

The mining and processing of naturally occurring radioactive materials (NORMs) could result in elevated levels of natural radionuclides in the environment. The gold mining in the goldfields of the Witwatersrand Basin of South Africa has resulted in numerous tailing dams that have high concentrations of NORM bearing residue. The aim of this study was to evaluate the radioactivity levels in tailing dams, soils and rocks, and the consequential radiological exposure to the public in the gold mining areas of Gauteng Province, South Africa. The activity concentrations of 238U, 232Th, and 40K were assessed using a gamma ray spectrometer (RS-230), and the activity concentrations ranges in the mine tailings were 209.95–2578.68 Bq/kg, 19.49–108.00 Bq/kg, and 31.30–626.00 Bq/kg, respectively. The radionuclides show significant spatial variability in soils, with high activities recorded in soils located in close proximity to tailings although regionally, the soil radioactivity levels mainly depend on the chemistry of the underlying rocks. The estimated annual effective doses were higher than the recommended regulatory limit of 0.25 mSv/y in particular tailing dams and soil impacted by tailings. Therefore, to ensure the protection of people and the environment, further monitoring and regulatory control measures targeting these areas are required.
3D Geophysical Predictive Modeling by Spectral Feature Subset Selection in Mineral Exploration
Authors: Abbassi Bahman , Cheng Li-Zhen , Jébrak Michel , Lemire Daniel
Published: October 2022

Several technical challenges are related to data collection, inverse modeling, model fusion, and integrated interpretations in the exploration of geophysics. A fundamental problem in integrated geophysical interpretation is the proper geological understanding of multiple inverted physical property images. Tackling this problem requires high-dimensional techniques for extracting geological information from modeled physical property images. In this study, we developed a 3D statistical tool to extract geological features from inverted physical property models based on a synergy between independent component analysis and continuous wavelet transform. An automated interpretation of multiple 3D geophysical images is also presented through a hybrid spectral feature subset selection (SFSS) algorithm based on a generalized supervised neural network algorithm to rebuild limited geological targets from 3D geophysical images. Our self-proposed algorithm is tested on an Au/Ag epithermal system in British Columbia (Canada), where layered volcano-sedimentary sequences, particularly felsic volcanic rocks, are associated with mineralization. Geophysical images of the epithermal system were obtained from 3D cooperative inversion of aeromagnetic, direct current resistivity, and induced polarization data sets. The recovered cooperative susceptibilities allowed locating a magnetite destructive zone associated with porphyritic intrusions and felsic volcanoes (Au host rocks). The practical implementation of the SFSS algorithm in the study area shows that the proposed spectral learning scheme can efficiently learn the lithotypes and Au grade patterns and makes predictions based on 3D physical property inputs. The SFSS also minimizes the number of extracted spectral features and tries to pick the best representative features for each target learning case. This approach allows interpreters to understand the relevant and irrelevant spectral features in addition to the 3D predictive models. Compared to conventional 3D interpolation methods, the 3D lithology and Au grade models recovered with SFSS add predictive value to the geological understanding of the deposit in places without access to prior geological and borehole information.
Partial Least Squares-Discriminant Analysis of the Major and Trace Elements and their Evolutionary Characteristics from the Jinchuan Ni-Cu-(PGE) Sulfide Deposit, NW China
Authors: Wang Yuhua , Lai Jianqing , Cao Yonghua , Mao Xiancheng , Liu Xianghua , Peng Lu , Ai Qixing
Published: October 2022

The world-renowned Jinchuan Cu-Ni-(PGE) sulfide deposit consists of four mainly independent intrusive units from west to east, namely Segments III, I, II-W, and II-E, and the main sulfide types are the disseminated, net-textured, massive, and Cu-rich ores. Due to the similar geochemical characteristics of each segment, there is no convenient method to distinguish them and explain their respective variations. Meanwhile, considering that the division of different types of ores is confusing and their formation is still controversial, direct classification using elemental discrimination maps can facilitate subsequent mining and research. In this paper, we report the new major and trace elements data from the Jinchuan deposit and collect the published data to construct a database of 10 major elements for 434 samples and 33 trace elements for 370 samples, respectively, and analyze the data based on multivariate statistical analysis for the first time. Robust estimation of compositional data (robCompositions) was applied to investigate censored geochemical data, and the input censored data were transformed using the centered log-ratios (clr) to overcome the closure effect on compositional data. Exploratory data analysis (EDA) was used to characterize the spatial distribution and internal structural features of the data. The transformed data were classified by partial least squares-discriminant analysis (PLS-DA) to identify different compositional features for each segment and ore type. The receiver operator characteristic (ROC) curve was used to verify the model results, which showed that the PLS-DA model we constructed was reliable. The main discriminant elements were obtained by PLS-DA of the major and trace elements, and based on these elements, we propose the plot of SiO2 + Al2O3 vs. CaO + Na2O + K2O and Cs + Ce vs. Th + U to discriminate the different segments of the Jinchuan deposit, and the Al2O3 + CaO vs. Fe2O3T + Na2O and Co + Cu vs. Rb + Th + U to discriminate the different ore types. In addition, we predict that there are still considerable metal reserves at the bottom of Segment I.
Bio-Geochemical Processes: Insights from Fe-Mn Mineralization in the Aegean Sea (Greece)
Authors: Vasilatos Charalampos , Kampouroglou Evdokia E. , Megremi Ifigeneia , Economou-Eliopoulos Maria
Published: October 2022

In this study, we have compiled new and existing mineralogical and geochemical data on Fe-Mn mineralization from the Aegean region [Attica (Grammatiko, Legrena, and Varnavas), Evia and Milos islands], aiming to provide new insights on the genesis of Fe-Mn mineralization in that region and its potential environmental implications. A common feature of those deposits is the relatively low Cr, Co, V, Ni, Mo, and Cd content, whereas Ba, As, W, Cu, Pb, and Zn show remarkably variable values. The Mn-Fe deposits from Milos exhibit the highest tungsten content, while a positive trend between MnO and W, combined with a negative trend between MnO and Fe2O3 suggests the preference of W to Mn-minerals. The occurrence of bacterio-morphic Fe-Mn-oxides/hydroxides within Mn-Fe mineralizations in the studied region, indicates the important role of micro-organisms into redox reactions. Moreover, the presence of micro-organisms in the Fe-Mn-deposits, reflecting the presence of organic matter confirms a shallow marine environment for their deposition. A salient feature of the Varnavas and Milos Mn-Fe ores is the presence of sodium chloride coated fossilized micro-organisms, suggesting development from a solution containing relatively high Na and Cl concentrations. Furthermore, from an environmental point of view, consideration is given to the bioavailability of elements such as As, Pb, and W, related to the above-mentioned mineralizations. The high bio-accumulation factor for W (Wplant/Wsoil × 100) recorded in the Neogene sedimentary basins of Attica, related to the Grammatiko Fe-Mn mineralization, reflects the high W mobility under alkaline conditions and the potential environmental impact of similar deposits with elevated W content.
Chemistry and Crystallization Conditions of Minerals in Metasomatized Oceanic Lithosphere and Basaltic Rocks of Govorov Guyot, Magellan Seamounts, Pacific Ocean
Authors: Peretyazhko Igor S. , Savina Elena A.
Published: October 2022

Studies have provided first detailed data on the chemistry of rock-forming, minor, and accessory minerals of Govorov Guyot volcanic rocks (basalts, trachybasalts, basaltic trachyandesites, and trachyandesites). Some basalt samples bear pargasitic amphibole and clinopyroxene xenocrysts, mantle vein fragments in xenoliths, as well as wehrlite xenoliths, which are remnants of metasomatized oceanic lithosphere. Amphiboles make up a continuous series from pargasite –Mg-hastingsite in wehrlite xenoliths and xenocrysts to Mg-hastingsite–kaersutite end-members in phenocrysts and microlites of basaltic rocks. The discussed issues include the trace element chemistry of Ti-amphibole and clinopyroxene phenocrysts; fractionation of OIB melts; and P-T equilibration of minerals during the formation of mantle and basaltic rocks. Pargasitic amphibole may have crystallized at P-T conditions (2.5–0.6 GPa, 1170–980 °C) corresponding to the spinel facies of peridotite at different depths (73–21 km) in hydrous (6.0–4.5 wt% H2O) silicate mafic melts that percolated through peridotites of the oceanic lithosphere. Ti-amphibole in basaltic rocks crystallized at 1.2–0.4 GPa (40–15 km), and 1060–910 °C from melts containing 8.6–2.6 wt% H2O. As the high-temperature (~1100 °C) basaltic magmas reached chambers at the oceanic crust level (7 to 3 km), the Ti-bearing amphiboles of xenocrysts and phenocrysts became replaced by Ti-magnetite- and/or rhönite-bearing mineral assemblages.
TSR Action and Genesis Mechanism of Antimony Deposit: Evidence from Aromatic Hydrocarbon Geochemistry of Bitumen from Paleo-Oil Reservoir in Qinglong Ore Field, Southwestern Guizhou Depression, China
Authors: Cheng Yong , Hu Yuzhao , Xu Saihua , Wang Di
Published: October 2022

In Qinglong ore field, the paleo-oil reservoir is found to be associated with antimony deposits, and they have a close genetic relationship. In this study, the aromatics geochemistry of paleo-oil reservoir bitumen was studied to further discuss the thermochemical sulfate reduction (TSR) reaction and the mechanism of antimony mineralization. A total of 124 aromatic compounds were identified by gas chromatography–mass spectrometry (GC–MS) analysis in bitumen samples, including abundant phenanthrene series, dibenzothiophene series, fluoranthene series, chrysene series, and a small number of fluorene series, naphthalene series, dibenzofuran series, biphenyl series, and triaromatic steroid series. Aromatic parameters such as trimethylnaphthalene index (TMNr), methylphenanthrene index (MPI), methylphenanthrene distribution fraction (MPDF, F1, and F2), methyldibenzothiophene parameter (MDR), C28TAS-20S/(20R + 20S), and benzofluoranthene/benzo[e]pyrene indicate that the Qinglong paleo-oil reservoir is in over maturity level. The abundance of phenanthrene and chrysene aromatic compounds and a small amount of naphthalene series, benzofluoranthene, fluoranthene, pyrene, anthracene, retene, perylene, and biphenyl suggest that the organic matter source of the paleo-oil reservoir was mainly low aquatic organisms, mixed with a small amount of higher plant. They detected a certain number of compounds, such as retene, triaromatic steroid series, and perylene, the ternary diagram of DBF–DBT–F and binary plot of Pr/Ph–DBT/P, DBT/(F + DBT)–DBF/(F + DBF), and Pr/Ph–DBT/DBF reveal that the source rock of the paleo-oil reservoir was formed in the marine environment of weak oxidation and weak reduction. The comprehensive analysis shows that the Qinglong paleo-oil reservoir originated from Devonian source rocks, just like other paleo-oil reservoirs and natural gas reservoirs in the Nanpanjiang basin. Abundant dibenzothiophene series were detected, indicating that the paleo-oil reservoir underwent a certain degree of TSR reaction. We believe that the gas reservoir formed by the evolution of the oil reservoir in the ore field participated in antimony mineralization; that is, hydrocarbon organic matter acted as a reducing agent and transformed SO42− in oilfield brine into H2S through TSR, providing reduced sulfur and creating environmental conditions for mineralization.