bluuebunny/crossref_metadata_2025 huggingface dataset:
Biogenic synthesis, characterization, and evaluation of synthesized nanoparticles against the pathogenic fungus Alternaria solani
Authors: Narware Jeetu , Singh Satyendra P. , Manzar Nazia , Kashyap Abhijeet Shankar
Published: April 2023

In the present study, Trichoderma harzianum culture filtrate (CF) was used as a reducing and capping agent to synthesize silver nanoparticles (Ag NPs) in a quick, simple, cost-effective, and eco-friendly manner. The effects of different ratios (silver nitrate (AgNO3): CF), pH, and incubation time on the synthesis of Ag NPs were also examined. Ultraviolet–visible (UV–Vis) spectra of the synthesized Ag NPs showed a distinct surface plasmon resonance (SPR) peak at 420 nm. Spherical and monodisperse NPs were observed using scanning electron microscopy (SEM). Elemental silver (Ag) was identified in the Ag area peak indicated by energy dispersive x-ray (EDX) spectroscopy. The crystallinity of Ag NPs was confirmed by x-ray diffraction (XRD), and Fourier transform infrared (FTIR) was used to examine the functional groups present in the CF. Dynamic light scattering (DLS) revealed an average size (43.68 nm), which was reported to be stable for 4 months. Atomic force microscopy (AFM) was used to confirm surface morphology. We also investigated the in vitro antifungal efficacy of biosynthesized Ag NPs against Alternaria solani, which demonstrated a significant inhibitory effect on mycelial growth and spore germination. Additionally, microscopic investigation revealed that Ag NP-treated mycelia exhibited defects and collapsed. Apart from this investigation, Ag NPs were also tested in an epiphytic environment against A. solani. Ag NPs were found to be capable of managing early blight disease based on field trial findings. The maximum percentage of early blight disease inhibition by NPs was observed at 40 parts per million (ppm) (60.27%), followed by 20 ppm (58.68%), whereas in the case of the fungicide mancozeb (1,000 ppm), the inhibition was recorded at 61.54%.
Pan-genome wide association study of Glaesserella parasuis highlights genes associated with virulence and biofilm formation
Authors: Zhou You , Jiang Dike , Yao Xueping , Luo Yan , Yang Zexiao , Ren Meishen , Zhang Ge , Yu Yuanyuan , Lu Aiping , Wang Yin
Published: April 2023

Glaesserella parasuisis a gram-negative bacterium that causes fibrotic polyserositis and arthritis in pig, significantly affecting the pig industry. The pan-genome ofG. parasuisis open. As the number of genes increases, the core and accessory genomes may show more pronounced differences. The genes associated with virulence and biofilm formation are also still unclear due to the diversity ofG. parasuis. Therefore, we have applied a pan-genome-wide association study (Pan-GWAS) to 121 strainsG. parasuis. Our analysis revealed that the core genome consists of 1,133 genes associated with the cytoskeleton, virulence, and basic biological processes. The accessory genome is highly variable and is a major cause of genetic diversity inG. parasuis. Furthermore, two biologically important traits (virulence, biofilm formation) ofG. parasuiswere studiedviapan-GWAS to search for genes associated with the traits. A total of 142 genes were associated with strong virulence traits. By affecting metabolic pathways and capturing the host nutrients, these genes are involved in signal pathways and virulence factors, which are beneficial for bacterial survival and biofilm formation. This research lays the foundation for further studies on virulence and biofilm formation and provides potential new drug and vaccine targets againstG. parasuis.
Bioinformatic identification of ClpI, a distinct class of Clp unfoldases in Actinomycetota
Authors: Jiang Jialiu , Schmitz Karl R.
Published: April 2023

All clades of bacteria possess Hsp100/Clp family unfoldase enzymes that contribute to aspects of protein quality control. In Actinomycetota, these include ClpB, which functions as an independent chaperone and disaggregase, and ClpC, which cooperates with the ClpP1P2 peptidase to carry out regulated proteolysis of client proteins. We initially sought to algorithmically catalog Clp unfoldase orthologs from Actinomycetota into ClpB and ClpC categories. In the process, we uncovered a phylogenetically distinct third group of double-ringed Clp enzymes, which we term ClpI. ClpI enzymes are architecturally similar to ClpB and ClpC, with intact ATPase modules and motifs associated with substrate unfolding and translation. While ClpI possess an M-domain similar in length to that of ClpC, its N-terminal domain is more variable than the strongly conserved N-terminal domain of ClpC. Surprisingly, ClpI sequences are divisible into sub-classes that either possess or lack the LGF-motifs required for stable assembly with ClpP1P2, suggesting distinct cellular roles. The presence of ClpI enzymes likely provides bacteria with expanded complexity and regulatory control over protein quality control programs, supplementing the conserved roles of ClpB and ClpC.
A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains
Authors: Zhao Yang , Wei Hua-Mei , Yuan Jia-Li , Xu Lian , Sun Ji-Quan
Published: April 2023

Acinetobacter is ubiquitous, and it has a high species diversity and a complex evolutionary pattern. To elucidate the mechanism of its high ability to adapt to various environment, 312 genomes of Acinetobacter strains were analyzed using the phylogenomic and comparative genomics methods. It was revealed that the Acinetobacter genus has an open pan-genome and strong genome plasticity. The pan-genome consists of 47,500 genes, with 818 shared by all the genomes of Acinetobacter, while 22,291 are unique genes. Although Acinetobacter strains do not have a complete glycolytic pathway to directly utilize glucose as carbon source, most of them harbored the n-alkane-degrading genes alkB/alkM (97.1% of tested strains) and almA (96.7% of tested strains), which were responsible for medium-and long-chain n-alkane terminal oxidation reaction, respectively. Most Acinetobacter strains also have catA (93.3% of tested strains) and benAB (92.0% of tested strains) genes that can degrade the aromatic compounds catechol and benzoic acid, respectively. These abilities enable the Acinetobacter strains to easily obtain carbon and energy sources from their environment for survival. The Acinetobacter strains can manage osmotic pressure by accumulating potassium and compatible solutes, including betaine, mannitol, trehalose, glutamic acid, and proline. They respond to oxidative stress by synthesizing superoxide dismutase, catalase, disulfide isomerase, and methionine sulfoxide reductase that repair the damage caused by reactive oxygen species. In addition, most Acinetobacter strains contain many efflux pump genes and resistance genes to manage antibiotic stress and can synthesize a variety of secondary metabolites, including arylpolyene, β-lactone and siderophores among others, to adapt to their environment. These genes enable Acinetobacter strains to survive extreme stresses. The genome of each Acinetobacter strain contained different numbers of prophages (0–12) and genomic islands (GIs) (6–70), and genes related to antibiotic resistance were found in the GIs. The phylogenetic analysis revealed that the alkM and almA genes have a similar evolutionary position with the core genome, indicating that they may have been acquired by vertical gene transfer from their ancestor, while catA, benA, benB and the antibiotic resistance genes could have been acquired by horizontal gene transfer from the other organisms.
Microbial succession and its effect on the formation of umami peptides during sufu fermentation
Authors: Mao Jieqi , Zhou Zhilei , Yang Hongshun
Published: April 2023

Sufu, a traditional Chinese fermented food, is famous for its unique flavor, especially umami. However, the formation mechanism of its umami peptides is still unclear. Here, we investigated the dynamic change of both umami peptides and microbial communities during sufu production. Based on peptidomic analysis, 9081 key differential peptides were identified, which mainly involved in amino acid transport and metabolism, peptidase activity and hydrolase activity. Twenty-six high-quality umami peptides with ascending trend were recognized by machine learning methods and Fuzzy c-means clustering. Then, through correlation analysis, five bacterial species (Enterococcus italicus, Leuconostoc citreum, L. mesenteroides, L. pseudomesenteroides, Tetragenococcus halophilus) and two fungi species (Cladosporium colombiae, Hannaella oryzae) were identified to be the core functional microorganisms for umami peptides formation. Functional annotation of five lactic acid bacteria indicated their important functions to be carbohydrate metabolism, amino acid metabolism and nucleotide metabolism, which proved their umami peptides production ability. Overall, our results enhanced the understanding of microbial communities and the formation mechanism of umami peptides in sufu, providing novel insights for quality control and flavor improvement of tofu products.
Vitamin D deficiency promoting non-24 h sleep–wake disorder: a case report
Authors: Radlberger Richard Friedrich , Kunz Alexander Baden
Published: April 2023

We report a case of an emmetropic woman with excessive daytime sleepiness in alternation with insomnia consistent with the diagnosis criteria of a non-24 h sleep–wake disorder. After being refractory to the usual non-pharmacologic and pharmacologic treatment, we detected a deficiency of vitamin B12, vitamin D3, and folic acid. Substitution of these treatments led to a return of a 24 h sleep–wake rhythm though this remained independent from the external light–dark cycle. The question arises whether the vitamin D deficiency could be regarded as an epiphenomenon or whether there is an up-to-date unknown connection to the inner zeitgeber.
Impact of non-motor fluctuations on QOL in patients with Parkinson’s disease
Authors: Kakimoto Asako , Kawazoe Miki , Kurihara Kanako , Mishima Takayasu , Tsuboi Yoshio
Published: April 2023

Introduction

Long-term levodopa treatment in patients with Parkinson’s disease (PwPD) often causes motor fluctuations, which are known to affect their quality of life (QOL). These motor fluctuations may be accompanied by fluctuations in non-motor symptoms. There is no consensus on how non-motor fluctuations affect QOL.

Methods

This was a single-center, retrospective study and included 375 patients with Parkinson’s disease (PwPD) who visited the neurology outpatient department of Fukuoka University Hospital between July 2015 and June 2018. All patients were evaluated for age, sex, disease duration, body weight, and motor symptoms by the Movement Disorder Society-Unified Parkinson’s Disease Rating Scale part III, depression scale by the Zung self-rating depression scale, apathy scale, and cognitive function by the Japanese version of The Montreal Cognitive Assessment. A nine-item wearing-off questionnaire (WOQ-9) was used to assess the motor and non-motor fluctuations. QOL in PwPD was investigated using the eight-item Parkinson’s Disease Questionnaire (PDQ-8).

Results

In total, 375 PwPD were enrolled and classified into three groups according to the presence or absence of motor and non-motor fluctuations. The first group included 98 (26.1%) patients with non-motor fluctuations (NFL group), the second group included 128 (34.1%) patients who presented with only motor fluctuations (MFL group), and the third group included 149 (39.7%) patients without fluctuations in motor or non-motor symptoms (NoFL group). Among them, the PDQ-8 SUM and SI were significantly higher in the NFL group than in the other groups (p < 0.005), implying that the NFL group had the poorest QOL among groups. Next, multivariable analysis showed that even one non-motor fluctuation was an independent factor that worsened QOL (p < 0.001).

Conclusion

This study showed that PwPD with non-motor fluctuation had a lower QOL than those with no or only motor fluctuation. Moreover, the data showed that PDQ-8 scores were significantly reduced even with only one non-motor fluctuation.
Predictors of clinical outcomes in space-occupying cerebellar infarction undergoing suboccipital decompressive craniectomy
Authors: Lucia Kristin , Reitz Sarah , Hattingen Elke , Steinmetz Helmuth , Seifert Volker , Czabanka Marcus
Published: April 2023

Introduction

Despite current clinical guidelines recommending suboccipital decompressive craniectomy (SDC) in cerebellar infarction when patients present with neurological deterioration, the precise definition of neurological deterioration remains unclear and accurate timing of SDC can be challenging. The current study aimed at characterizing whether clinical outcomes can be predicted by the GCS score immediately prior to SDC and whether higher GCS scores are associated with better clinical outcomes.

Methods

In a single-center, retrospective analysis of 51 patients treated with SDC for space-occupying cerebellar infarction, clinical and imaging data were evaluated at the time points of symptom onset, hospital admission, and preoperatively. Clinical outcomes were measured by the mRS. Preoperative GCS scores were stratified into three groups (GCS, 3–8, 9–11, and 12–15). Univariate and multivariate Cox regression analyses were performed using clinical and radiological parameters as predictors of clinical outcomes.

Results

In cox regression analysis GCS scores of 12–15 at surgery were significant predictors of positive clinical outcomes (mRS, 1–2). For GCS scores of 3–8 and 9–11, no significant increase in proportional hazard ratios was observed. Negative clinical outcomes (mRS, 3–6) were associated with infarct volume above 6.0 cm3, tonsillar herniation, brainstem compression, and a preoperative GCS score of 3–8 [HR, 2.386 (CI, 1.160–4.906); p = 0.018].

Conclusion

Our preliminary findings suggest that SDC should be considered in patients with infarct volumes above 6.0 cm3 and with GCS between 12 and 15, as these patients may show better long-term outcomes than those in whom surgery is delayed until a GCS score below 11.
Hemispheric lateralization of white matter microstructure in children and its potential role in sensory processing dysfunction
Authors: Parekh Shalin A. , Wren-Jarvis Jamie , Lazerwitz Maia , Rowe Mikaela A. , Powers Rachel , Bourla Ioanna , Cai Lanya T. , Chu Robyn , Trimarchi Kaitlyn , Garcia Rafael , Marco Elysa J. , Mukherjee Pratik
Published: April 2023

Diffusion tensor imaging (DTI) studies have demonstrated white matter microstructural differences between the left and right hemispheres of the brain. However, the basis of these hemispheric asymmetries is not yet understood in terms of the biophysical properties of white matter microstructure, especially in children. There are reports of altered hemispheric white matter lateralization in ASD; however, this has not been studied in other related neurodevelopmental disorders such as sensory processing disorder (SPD). Firstly, we postulate that biophysical compartment modeling of diffusion MRI (dMRI), such as Neurite Orientation Dispersion and Density Imaging (NODDI), can elucidate the hemispheric microstructural asymmetries observed from DTI in children with neurodevelopmental concerns. Secondly, we hypothesize that sensory over-responsivity (SOR), a common type of SPD, will show altered hemispheric lateralization relative to children without SOR. Eighty-seven children (29 females, 58 males), ages 8–12 years, presenting at a community-based neurodevelopmental clinic were enrolled, 48 with SOR and 39 without. Participants were evaluated using the Sensory Processing 3 Dimensions (SP3D). Whole brain 3 T multi-shell multiband dMRI (b = 0, 1,000, 2,500 s/mm2) was performed. Tract Based Spatial Statistics were used to extract DTI and NODDI metrics from 20 bilateral tracts of the Johns Hopkins University White-Matter Tractography Atlas and the lateralization Index (LI) was calculated for each left–right tract pair. With DTI metrics, 12 of 20 tracts were left lateralized for fractional anisotropy and 17/20 tracts were right lateralized for axial diffusivity. These hemispheric asymmetries could be explained by NODDI metrics, including neurite density index (18/20 tracts left lateralized), orientation dispersion index (15/20 tracts left lateralized) and free water fraction (16/20 tracts lateralized). Children with SOR served as a test case of the utility of studying LI in neurodevelopmental disorders. Our data demonstrated increased lateralization in several tracts for both DTI and NODDI metrics in children with SOR, which were distinct for males versus females, when compared to children without SOR. Biophysical properties from NODDI can explain the hemispheric lateralization of white matter microstructure in children. As a patient-specific ratio, the lateralization index can eliminate scanner-related and inter-individual sources of variability and thus potentially serve as a clinically useful imaging biomarker for neurodevelopmental disorders.
Infection routes, invasion mechanisms, and drug inhibition pathways of human coronaviruses on the nervous system
Authors: Sha Ailong , Chen Hongrun
Published: April 2023

So far, numerous studies have reported on how coronaviruses affect the human nervous system. However, these studies mainly focused on the impact of a single coronavirus on the nervous system, and failed to fully report the invasion mechanisms and the rules of symptoms of the seven human coronaviruses. This research can assist medical professionals in identifying the regularity of coronavirus invasion into the nervous system by examining the impacts of human coronaviruses on the nervous system. Meanwhile, the discovery also helps humans to prevent the damage to the human nervous system caused by the more novel coronavirus in advance, thus reducing the rate of disease transmission and fatality caused by such viruses. In addition to describing the structures, routes of infection, and symptomatic manifestations of human coronaviruses, this review also finds that the structures of human coronaviruses correlate with virulence, pathways of infection, and blocking mechanisms of drugs. This review can provide a theoretical basis for the research and development of related drugs, promote the prevention and treatment of coronavirus infectious diseases, and contribute to global epidemic prevention.
Literature analysis on asparagus roots and review of its functional characterizations
Authors: Guo Yaodong , Liu Zhe , Wan Yingjie , Zhang Yanyan , Abdu Hassan Idris , Yang Meng , Pei Jinjin , Yue Tianli , Zhang Xianbin , Hacimuftuoglu Ahmet , Abd El-Aty A. M.
Published: April 2023

Asparagus root (AR) is utilized globally as a traditional herbal medicine because it contains various bioactive compounds, such as polyphenols, flavonoids, saponins, and minerals. The composition profiles of AR are strongly affected by its botanical and geographical origins. Although minerals and heavy metals are minor constituents of AR, they play a crucial role in determining its quality and efficacy. A comprehensive classification of AR, its phytochemistry, and its pharmacology were reviewed and interpreted herein. Potentially eligible articles (in English) were identified through an electronic search of the Web of Science database (2010–2022) and Google (2001–2022). We used the primary search term “Asparagus roots” combined with the words “pharmacology,” “bioactive compounds,” “physicochemical properties,” and “health benefits” to find the relevant literature. We screened the titles, keywords, and abstracts of the publications obtained from the database. A full copy of the article was obtained for further assessment if deemed appropriate. Different asparagus species might potentially be used as herbal medicines and functional foods. Phytochemical studies have revealed the presence of various bioactive compounds as valuable secondary metabolites. The dominant class of bioactive compounds in AR is flavonoids. Furthermore, AR displayed significant pharmacological effects, such as antioxidant, antimicrobial, antiviral, anticancer, anti-inflammatory, and antidiabetic effects, as shown in animal and human studies. This review provides a valuable resource to enable a thorough assessment of the profile of Asparagus root as a functional ingredient for the pharmaceutical and food industries. In addition, it is anticipated that this review will provide information to healthcare professionals seeking alternative sources of critical bioactive compounds.
Status of nutrition labeling knowledge, attitude, and practice (KAP) of residents in the community and structural equation modeling analysis
Authors: Liao Yinxia , Yang Jianjun
Published: April 2023

Objective

Unhealthy foods were a major contributor to the occurrence of chronic non-communicable diseases. The promotion of nutrition labeling in the community can effectively help residents to choose healthy foods, which plays an important role in the prevention of chronic diseases. However, the public awareness of this measure is not clear. Our study used a structural equation model based on the KAP theory to analyze the interaction mechanisms among knowledge, attitude, and practice and aimed to evaluate the relationships among nutrition knowledge, attitude, and practice of residents, which can provide the basis of policy formulation for nutrition education and behavior intervention.

Methods

We carried out a cross-sectional study from May 2022 to July 2022 in the “Community Health Service Center”, and each “Community Service Station” in Yinchuan use a self-designed questionnaire and convenience sampling to evaluate resident nutrition labeling KAP status. This study adopted the structural equation modeling approach to analyze a survey of Chinese individuals through the cognitive processing model, interrelated nutrition knowledge, nutrition label knowledge, attitude, and practice.

Results

According to the principle of sample size estimation, a total of 636 individuals were investigated, with the ratio of male to female being 1:1.2. The average score of community residents' nutrition knowledge was 7.48 ± 3.24, and the passing rate was 19.4%. Most residents had a positive attitude toward nutrition labeling, but the awareness rate was only 32.7% and the utilization rate was 38.5%. Univariate analysis showed that women had higher knowledge scores than men (p < 0.05), and young people had higher scores than older adults (p < 0.05), and the difference was significant. Based on the KAP structural equation model (SEM), residents' nutrition knowledge will directly affect their attitude toward nutrition labeling. Attitude played a greater role as an indirect effect between knowledge and behavior, while trust limits residents' practice of nutrition labeling and then affects their practice. It could be explained that nutrition knowledge was the prerequisite for label reading behavior, and attitude was the intermediary effect.

Conclusion

The nutrition knowledge and nutrition labeling knowledge of respondents hardly directly support the practice of nutrition labeling, but it can influence the use behavior by forming a positive attitude. The KAP model is suitable for explaining residents' use of nutrition labeling in the region. Future research should focus on better understanding the motivations of residents to use nutrition labeling and the opportunity to use nutrition labeling in real-life shopping settings.
Correlation between serum ferritin in early pregnancy and hypertensive disorders in pregnancy
Authors: Fang Zhuanji , Zheng Shuisen , Xie Yi , Lin Shunhe , Zhang Huale , Yan Jianying
Published: April 2023

Objective

To explore the correlation between serum ferritin (SF) in early pregnancy and the risk of hypertensive disorders in pregnancy (HDP).

Method

A retrospective cohort study was conducted on 43,421 pregnant women with singleton pregnancies who underwent antenatal checkups at Fujian Provincial Maternal and Child Health Hospital from January 2018 to December 2020. Based on pregnancy records, women were classified as non-hypertensive, having gestational hypertension, preeclampsia and preeclampsia with severe features according to the degree of the disease. General baseline data, and SF levels in the early (up to 12 gestational weeks) and late (after 28  weeks of gestation) stages of pregnancy were collected. The significance of the characteristic variables was assessed using a random forest algorithm, and the correlation between early pregnancy SF levels and the incidence of HDP was further analyzed using logistics regression adjusted for confounders. A generalized additive model (GAM) was fitted to a smoothed graph of the relationship between early pregnancy SF levels and HDP, and a threshold effect analysis was performed to find the threshold values of early pregnancy SF for iron supplementation therapy.

Result

A total of 30,703 pregnant women were included. There were 1,103 women who were diagnosed with HDP. Of them, 418 had gestational hypertension, 12 had chronic hypertension without SPE, 332 - preeclampsia and 341 women had preeclampsia with severe features. Levels of SF in early and late pregnancy were significantly higher (p < 0.001) in women with HDP compared to non-hypertensive women and the difference was more pronounced in early pregnancy. The random forest algorithm showed that early pregnancy SF was more effective in predicting HDP compared to late pregnancy SF levels and was also an independent risk factor for HDP (adjusted odds ratio (AOR) = 1.07, 95% CI [1.05,1.09]) after correction for confounding factors. Early pregnancy SF >64.22  mg/l was associated with higher risk of developing hypertensive disorders.

Conclusion

Risk of pregnancy-related hypertensive disorders increases with increasing early pregnancy SF levels. SF levels may therefore be used to further develop guidelines for iron supplementation therapy in pregnant women.
Higher dietary magnesium and potassium intake are associated with lower body fat in people with impaired glucose tolerance
Authors: Chu Natural , Chan Tsz Yeung , Chu Yuen Kiu , Ling James , He Jie , Leung Kathy , Ma Ronald C. W. , Chan Juliana C. N. , Chow Elaine
Published: April 2023

Introduction

Obesity and diabetes are public health concerns worldwide, but few studies have examined the habitual intake of minerals on body composition in people with prediabetes.

Methods

In this prospective cross-sectional study, 155 Chinese subjects with IGT [median age: 59 (53–62) years, 58% female] had an assessment of body composition including body fat percentage, oral glucose tolerance tests (OGTT), Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) and 3-day food records from nutritional programme analysis.

Results

Dietary intake of minerals was negatively correlated with body fat. People with obesity had the lowest daily consumption of iron median (IQR) 10.3 (6.9–13.3) mg, magnesium 224 (181–282) mg, and potassium 1973 (1563–2,357) mg when compared to overweight [10.5 (8.0–14.5) mg, 273 (221–335) mg, and 2,204 (1720–2,650) mg] and normal weight individuals [13.2 (10.0–18.6) mg, 313 (243–368) mg, and 2,295 (1833–3,037) mg] (p = 0.008, <0.0001, and 0.013 respectively). Amongst targeted minerals, higher dietary magnesium and potassium intake remained significantly associated with lower body fat after the adjustment of age, gender, macronutrients, fibre, and physical activity.

Conclusion

Dietary magnesium and potassium intake may be associated with lower body fat in people with impaired glucose tolerance. Inadequate dietary mineral intake may play contribute to obesity and metabolic disorders independent of macronutrients and fibre consumption.
Absolute quantification of a plasma tRNA-derived fragment for the diagnosis and prognosis of gastric cancer
Authors: Zhang Shuangshuang , Xie Yaoyao , Yu Xiuchong , Ge Jiaxin , Ye Guoliang , Guo Junming
Published: April 2023

Background

The transition from a healthy gastric mucosa to gastric cancer is a multi-step process. Early screening can significantly improve the survival rate of gastric cancer patients. A reliable liquid biopsy for gastric cancer prediction is urgently needed and since tRNA-derived fragments (tRFs) are abundant in various body fluids, tRFs are possible new biomarkers for gastric cancer.

Methods

A total of 438 plasma samples from patients with different gastric mucosal lesions as well as healthy individuals were collected. A specific reverse transcription primer, a forward primer, a reverse primer, and a TaqMan probe were designed. A standard curve was constructed and an absolute quantitation method was devised for detection of tRF-33-P4R8YP9LON4VDP in plasma samples of individuals with differing gastric mucosa lesions. Receiver operating characteristic curves were constructed to evaluate the diagnostic values of tRF-33-P4R8YP9LON4VDP for individual with differing gastric mucosa. A Kaplan–Meier curve was established to calculate the prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients. Finally, a multivariate Cox regression analysis was performed to assess the independent prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients.

Results

A detection method for plasma tRF-33-P4R8YP9LON4VDP was successfully established. Levels of plasma tRF-33-P4R8YP9LON4VDP were shown to reflect a gradient change from healthy individuals to gastritis patients to early and advanced gastric cancer patients. Significant differences were found among individuals with differing gastric mucosa, with reduced levels of tRF-33-P4R8YP9LON4VDP significantly related to a poor prognosis. tRF-33-P4R8YP9LON4VDP was found to be an independent predictor of an unfavorable survival outcome.

Conclusions

In this study, we developed a quantitative detection method for plasma tRF-33-P4R8YP9LON4VDP that exhibited hypersensitivity, convenience, and specificity. Detection of tRF-33-P4R8YP9LON4VDP was found to be a valuable means by which to monitor different gastric mucosa and to predict patient prognosis.
Real-world clinical outcomes of nivolumab and taxane as a second- or later-line therapy for recurrent or unresectable advanced esophageal squamous cell carcinoma
Authors: Ohsawa Manato , Hamai Yoichi , Emi Manabu , Ibuki Yuta , Kurokawa Tomoaki , Yoshikawa Toru , Hirohata Ryosuke , Kitasaki Nao , Okada Morihito
Published: April 2023

Background

Nivolumab is approved in Japan as a second-line treatment for patients with advanced esophageal squamous cell carcinoma (ESCC) resistant to fluoropyrimidine and platinum-based drugs. It is also used in adjuvant and primary postoperative therapies. This study aimed to report real-world data on nivolumab use for esophageal cancer treatment.

Methods

In total, 171 patients with recurrent or unresectable advanced ESCC who received nivolumab (n = 61) or taxane (n = 110) were included. We collected real-world data of patients treated with nivolumab as a second- or later-line therapy and evaluated treatment outcomes and safety.

Results

Median overall survival was longer and progression-free survival (PFS) was significantly longer (p = 0.0172) in patients who received nivolumab than in patients who received taxane as a second- or later-line therapy. Furthermore, subgroup analysis for second-line treatment only showed the superiority of nivolumab in increasing the PFS rate (p = 0.0056). No serious adverse events were observed.

Conclusions

In real-world practice, nivolumab was safer and more effective than taxane in patients with ESCC with diverse clinical profiles who did not meet trial eligibility criteria, including those with poor Eastern Cooperative Oncology Group performance status, comorbidities, and receiving multiple treatments.
Prognosis of lymphadenectomy in malignant ovarian germ cell tumor
Authors: Lv Bin , Liu Xinghui , Zheng Ai , Han Ling
Published: April 2023

Background and objectives

The routine application of lymphadenectomy remains a controversial part of surgical staging in malignant ovarian germ-cell tumor (MOGCT). Thus, studies are needed to explore the prognostic significance of lymphadenectomy in MOGCT. The goal of this retrospective study was to report the clinical outcomes of lymph node dissection (LND) and non-LND in MOGCT surgeries.

Measurements and main results

A total of 340 cases of MOGCTs were included: 143 patients (42.1%) had LND and 197 (57.9%) had no LND. The OS rates at 5 years in the LND and non-LND group were 99.3% vs. 100%, respectively. The DFS rates at 5 years in the LND and non-LND group were 88.8% vs. 88.3%. Forty-three patients (12.6%) were successfully pregnant during the postoperative follow-up. There were 44 recurrences (12.9%) and six deaths (1.8%). Stage was an independent prognostic factor for DFS in the multivariate analysis. Pathology was reported as an independent prognostic factor associated with OS in the multivariate analysis.

Conclusion

Lymphadenectomy had no significant influence on the OS (P=0.621) or disease-free survival rate (P=0.332) of patients with MOGCT.
Automated measurement of penile curvature using deep learning-based novel quantification method
Authors: Baray Sriman Bidhan , Abdelmoniem Mohamed , Mahmud Sakib , Kabir Saidul , Faisal Md. Ahasan Atick , Chowdhury Muhammad E. H. , Abbas Tariq O.
Published: April 2023

Objective

Develop a reliable, automated deep learning-based method for accurate measurement of penile curvature (PC) using 2-dimensional images.

Materials and methods

A set of nine 3D-printed models was used to generate a batch of 913 images of penile curvature (PC) with varying configurations (curvature range 18° to 86°). The penile region was initially localized and cropped using a YOLOv5 model, after which the shaft area was extracted using a UNet-based segmentation model. The penile shaft was then divided into three distinct predefined regions: the distal zone, curvature zone, and proximal zone. To measure PC, we identified four distinct locations on the shaft that reflected the mid-axes of proximal and distal segments, then trained an HRNet model to predict these landmarks and calculate curvature angle in both the 3D-printed models and masked segmented images derived from these. Finally, the optimized HRNet model was applied to quantify PC in medical images of real human patients and the accuracy of this novel method was determined.

Results

We obtained a mean absolute error (MAE) of angle measurement <5° for both penile model images and their derivative masks. For real patient images, AI prediction varied between 1.7° (for cases of ∼30° PC) and approximately 6° (for cases of 70° PC) compared with assessment by a clinical expert.

Discussion

This study demonstrates a novel approach to the automated, accurate measurement of PC that could significantly improve patient assessment by surgeons and hypospadiology researchers. This method may overcome current limitations encountered when applying conventional methods of measuring arc-type PC.
Quantitative structural analysis of hemifacial microsomia mandibles in different age groups
Authors: Zhang Ziwei , Chen Xiaojun , Kim Byeong Seop , Han Wenqing , Yan Yingjie , Wang Xuetong , Li Xin , Zhang Yan , Chai Gang
Published: April 2023

Introduction

This study aims to quantitively analyze mandibular ramus and body deformities, assessing the asymmetry and progression in different components.

Methods

This is a retrospective study on hemifacial microsomia children. They were divided into mild/severe groups by Pruzansky-Kaban classification and into three age groups (<1 year,1–5 years, 6–12 years old). Linear and volumetric measurements of the ramus and the body were collected via their preoperative imaging data to compare between the different sides and severities, using independent and paired tests, respectively. The progression of asymmetry was assessed by changes in affected/contralateral ratios with age using multi-group comparisons.

Results

Two hundred and ten unilateral cases were studied. Generally, the affected ramus and body were significantly smaller than those on the contralateral side. Linear measurements on the affected side were shorter in the severe group. Regarding affected/contralateral ratios, the body was less affected than the ramus. Progressively decreased affected/contralateral ratios of body length, dentate segment volume, and hemimandible volume were found.

Discussion

There were asymmetries in mandibular ramus and body regions, which involved the ramus more. A significant contribution to progressive asymmetry from the body suggests treatment focus in this region.
Case report: Gastroenterological management in a case of cardio-facio-cutaneous syndrome
Authors: Ciacchini B. , Di Nardo G. , Marin M. , Borali E. , Caraccia M. , Mogni R. , Cairello F. , Rabbone I. , Ferrero G. B , Pini Prato A. , Felici E.
Published: April 2023

Background

cardio-facio-cutaneous syndrome is a rare genetic disorder affecting less than 900 people in the world. It is mainly characterized by craniofacial, dermatologic and cardiac defects, but also gastroenterological symptoms may be present, ranging from feeding difficulties to gastroesophageal reflux and constipation.

In this report we describe a case of this syndrome characterized by severe feeding and growth difficulties, with a particular focus on the management of gastroenterological complications.

Case presentation

the patient was a caucasian male affected by Cardio-Facio-Cutaneous syndrome who presented feeding difficulties already a few hours after birth. These symptoms worsened in the following months and lead to a complete growth arrest and malnutrition. He was first treated with a nasogastric tube placement. Subsequently, a laparoscopic Nissen fundoplication and a laparoscopic Stamm gastrostomy were performed. The child was fed with nocturnal enteral nutrition and diurnal oral and enteral nutrition. Eventually the patient resumed feeding validly and regained adequate growth.

Conclusion

this paper aims to bring to light a complex rare syndrome that infrequently comes to the attention of the pediatricians and whose diagnosis is not always straightforward. We also highlight the possible complications under a gastroenterologic point of view. Our contribution can be helpful to the pediatrician in the first diagnostic suspect of this syndrome. In particular, it is worth highlighting that -in an infant with Noonan-like features- symptoms like suction or swallowing problems, vomiting and feeding difficulties should orient towards the diagnosis of a Cardio-facio-cutaneous syndrome. It is also important to stress that its related gastroenterological issues may lead to severe growth failure and therefore the role of the gastroenterologist is key to manage supplemental feeding and to establish whether a nasogastric or gastrostomic tube placement is necessary.
Relationship between global identity and pro-environmental behavior and environmental concern: a systematic review
Authors: Pong Vivien , Tam Kim-Pong
Published: April 2023

Global issues such as environmental problems and climate change, require collective efforts. Global identity has been linked to the promotion of pro-environmental behavior by international and environmental organizations. In environment-related research, this all-inclusive social identity has been consistently related to pro-environmental behavior and environmental concern, but the underlying mechanisms are not well understood. This current systematic review seeks to examine past studies across disciplines that have reported findings on the relationship between global identity and the constructs of pro-environmental behavior and environmental concern and to synthesize findings on the potential pathways behind this relationship. Thirty articles were identified through a systematic search. We found that most studies reported a positive correlation, and the effect of global identity on pro-environmental behavior and environmental concern was stable across studies. Only nine of the studies empirically examined the underlying mechanisms of this relationship. Three major themes of these underlying mechanisms emerged: obligation, responsibility, and relevance. These mediators highlight the role of global identity in pro-environmental behavior and environmental concern via how individuals relate to other humans and how they appraise environmental problems. We also observed a heterogeneity in measurements of global identity and environment-related outcomes. As a topic of interest in multiple disciplines, a variety of global identity labels have been adopted, such as global identity, global social identity, humanity identity, Identification With All Humanity, global/world citizen, connectedness to humanity, global belonging, and psychological sense of global community. Self-report measures of behavior were common, but observations of actual behavior were rare. Knowledge gaps are identified, and future directions are suggested.
Empowering Chinese college students in English as a foreign language writing classes: Translanguaging with translation methods
Authors: Zhang Limei
Published: April 2023

Learning activities based on translation has attracted increasing attention among researchers in English as a Foreign Language (EFL) teaching and learning under the influence of translanguaing pedagogies. This study was designed to examine the effect of translation methods as pedagogical tools on students’ writing performance in EFL classrooms. 89 Chinese college students participated in the study. They were required to complete tests of essay writing before and after the use of the translation method. Nine students were invited to attend an interview after the writing test. Results showed that the students improved their essay writing performance significantly after the translation method. The participating students’ confidence and interest in essay writing were also enhanced. Findings from the study have important implications for effective writing instruction for Chinese EFL college students.
With a little help from my friends: adopting a P-E fit perspective in understanding the value of organizational learning climate for sustainable employability
Authors: Vuuren Tinka Van , Van der Heijden Beatrice I. J. M. , Semeijn Judith H.
Published: April 2023

Purpose

The objective of our study was to investigate how organizational learning climate (measured as developmental opportunities and team support for learning), career commitment, and age are related to employees’ self-perceived employability, vitality and work ability (e.g., their sustainable employability). Our study adopted a P-E fit perspective building upon the notion that sustainable employability is a function of both the person (P) and the environment (E) and tests a three-way interaction between organizational learning climate, career commitment, and age.

Design

In total, 211 members of the support staff of a Dutch university completed a survey. Hierarchical stepwise regression analysis was used to analyze the data.

Findings

Only one of the two dimensions of organizational learning climate that we measured, namely the developmental opportunities, appeared to be associated with all indicators of sustainable employability. Career commitment only had a direct positive relationship with vitality. Age was negatively related to self-perceived employability and to work ability, but not to vitality. The relationship between developmental opportunities and vitality was negatively influenced by career commitment (a negative two-way interaction effect), while a positive three-way interaction effect was found between career commitment, age, and development opportunities, and with self-perceived employability as the outcome.

Theoretical and practical implications

Our findings confirmed the relevance of adopting a P-E fit perspective on sustainable employability, and of considering the possible role of age in this. It requires more detailed analyses in future research to unravel the role of age in the shared responsibility for sustainable employability. In practice, the results of our study imply that organizations should provide all employees with a working context that facilitates learning, however, with a special focus on older employees, for whom it is a particular challenge to protect their sustainable employability, possibly due to age-related stereotyping.

Originality

Our study adopted a P-E fit perspective on sustainable employability and examined the association between organizational learning climate and all three components of sustainable employability: self-perceived employability, vitality and work ability. Moreover, it investigated whether and how the employee’s career commitment and age influence this relationship.
Modeling the function of episodic memory in spatial learning
Authors: Zeng Xiangshuai , Diekmann Nicolas , Wiskott Laurenz , Cheng Sen
Published: April 2023

Episodic memory has been studied extensively in the past few decades, but so far little is understood about how it drives future behavior. Here we propose that episodic memory can facilitate learning in two fundamentally different modes: retrieval and replay, which is the reinstatement of hippocampal activity patterns during later sleep or awake quiescence. We study their properties by comparing three learning paradigms using computational modeling based on visually-driven reinforcement learning. Firstly, episodic memories are retrieved to learn from single experiences (one-shot learning); secondly, episodic memories are replayed to facilitate learning of statistical regularities (replay learning); and, thirdly, learning occurs online as experiences arise with no access to memories of past experiences (online learning). We found that episodic memory benefits spatial learning in a broad range of conditions, but the performance difference is meaningful only when the task is sufficiently complex and the number of learning trials is limited. Furthermore, the two modes of accessing episodic memory affect spatial learning differently. One-shot learning is typically faster than replay learning, but the latter may reach a better asymptotic performance. In the end, we also investigated the benefits of sequential replay and found that replaying stochastic sequences results in faster learning as compared to random replay when the number of replays is limited. Understanding how episodic memory drives future behavior is an important step toward elucidating the nature of episodic memory.
Network analysis of the relationships between problematic smartphone use and anxiety, and depression in a sample of Chinese college students
Authors: Guo Zhihua , Yang Tianqi , Qiu Rui , Qiu Huake , Ren Lei , Liu Xufeng , Han Zheyi , Zhu Xia
Published: April 2023

Background

Problematic smartphone use (PSU) is associated with both anxiety and depression. However, the relationships between components of PSU and symptoms of anxiety or depression have not been investigated. Hence, the aim of this study was to closely examine the relationships between PSU and anxiety and depression to identify the pathological mechanisms underpinning those relationships. A second aim was to identify important bridge nodes to identify potential targets for intervention.

Methods

Symptom-level network structures of PSU and anxiety, and PSU and depression were constructed to investigate the connections between the variables and evaluate the bridge expected influence (BEI) of each node. Network analysis using data from 325 Chinese healthy college students was performed.

Results

Five strongest edges appeared within the communities in both the PSU-anxiety and PSU-depression networks. The “Withdrawal” component had more connections with symptoms of anxiety or depression than any other PSU node. In particular, the edges between “Withdrawal” and “Restlessness” and between “Withdrawal” and “Concentration difficulties” were the strongest cross-community edges in the PSU-anxiety network and PSU-depression network, respectively. Furthermore, “Withdrawal” had the highest BEI in the PSU community in both networks.

Conclusions

These findings provide preliminary evidence of the pathological pathways linking PSU with anxiety and depression, with “Withdrawal” linking PSU with both anxiety and depression. Hence, “Withdrawal” may be a potential target for preventing and intervening in cases of anxiety or depression.