bluuebunny/crossref_metadata_2025 huggingface dataset:
Real sounds influence postural stability in people with vestibular loss but not in healthy controls
Authors: Lubetzky Anat V. , Cosetti Maura , Harel Daphna , Sherrod Marlee , Wang Zhu , Roginska Agnieszka , Kelly Jennifer
Published: January 2025

Objective

What we hear may influence postural control, particularly in people with vestibular hypofunction. Would hearing a moving subway destabilize people similarly to seeing the train move? We investigated how people with unilateral vestibular hypofunction and healthy controls incorporated broadband and real-recorded sounds with visual load for balance in an immersive contextual scene.

Design

Participants stood on foam placed on a force-platform, wore the HTC Vive headset, and observed an immersive subway environment. Each 60-second condition repeated twice: static or dynamic visual with no sound or static white noise or real recorded subway station sounds [real] played from headphones.

Setting

Human motion laboratory.

Participants

41 healthy controls (mean age 52 years, range 22–78) and 28 participants with unilateral peripheral vestibular hypofunction (mean age 61.5, 27–82)

Main outcome measures

We collected center-of-pressure (COP, anterior-posterior, medio-lateral) from the force-platform and head (anterior-posterior, medio-lateral, pitch, yaw, roll) from the headset and quantified root mean square velocity (cm/s or rad/s)

Results

Adjusting for age, the vestibular group showed significantly more sway than controls on: COP medio-lateral (no sound or real with static or dynamic visual); COP anterior-posterior (only on dynamic visuals in the presence of either sound); head medio-lateral and anterior-posterior (all conditions), head pitch and yaw (only on dynamic visuals in the presence of either sound). A significant increase in sway with sounds was observed for the vestibular group only on dynamic visuals COP anterior-posterior and head yaw (real) and head anterior-posterior and pitch (either sound).

Conclusions

The addition of auditory stimuli, particularly contextually-accurate sounds, to a challenging, standing balance task in real-life simulation increased sway in people with vestibular hypofunction but not in healthy controls.

Trial registration

Clinical trial registration

This study was registered on clinicaltrials.gov at the following link: https://clinicaltrials.gov/study/NCT04479761.
Female genital mutilation among children in Ethiopia: A time-to-event analysis of age at circumcision
Authors: Ofori Michael Arthur , Bekalo Daniel Biftu , Mensah David Kwamena , Jha Niharika
Published: January 2025

Female Genital Mutilation (FGM) has become a global health concern. It is a deeply entrenched harmful practice involving partial or total removal of the external female genitalia for non-medical reasons. To inform effective policymaking and raise awareness about FGM’s health risks, understanding socioeconomic and demographic factors influencing the timing of girls’ circumcision is crucial. This study employed semi-parametric survival models to examine the association between residential status and time-to-circumcision of girls in Ethiopia. The data used in this study was the 2016 Ethiopian Demographic and Health Survey (EDHS) report, which was conducted by the Central Statistical Agency (CSA). The analysis revealed that residential status (rural vs urban, HR = 1.73: 1.35–2.70), religion (Muslim vs Christian, HR = 1.51: 1.38–2.70), mother’s educational level (none vs higher, HR = 5.25: 2.23–12.36 or primary vs higher HR = 4.12: 1.25–9.68), father’s educational level (none vs higher, HR = 1.65: 1.12–2.43), and mother’s age (15–24 years vs 35 +, HR = 1.89: 1.21–2.95 or 24–34 years vs 35 +, HR = 1.55: 1.18–2.02) are significant risk factors for age at circumcision. Mother’s age and family wealth index were time dependent risk factors. Effective interventions to reduce FGM prevalence would need to address multiple aspects simultaneously, including improving access to education (especially for girls and women), targeting both urban and rural areas with awareness campaigns, and considering culturally sensitive approaches that take into account religious and traditional beliefs while promoting the health and rights of girls and women.
Validation of the somatic symptom disorder—B Criteria Scale (SSD-12) in Bangladesh
Authors: Habib Sumaiya , Mozumder Muhammad Kamruzzaman
Published: January 2025

Background

The absence of a reliable and valid Bangla instrument for measuring somatic symptom disorder hinders research and clinical activities in Bangladesh. The present study aimed at translating and validating the Somatic Symptom Disorder-B criteria (SSD-12).

Method

A cross-sectional design was used with purposively selected clinical (n = 100) and non-clinical (n = 100) samples. The clinical sample was collected from psychiatric departments at three hospitals, while the non-clinical sample was drawn from the local community. Exploratory (EFA) and confirmatory factor analysis (CFA) were conducted on the SSD-12, along with reliability and validity assessments.

Results

Results indicated satisfactory internal consistency reliability (Cronbach’s alpha = .94, split-half r = .93); criterion-related validity (r = .86, with Morey’s Somatic Complaints Scale); and construct validity (r = .64 with anxiety subscale and r = .57 with depression subscale of the Hospital Anxiety and Depression Scale) of the translated scale. In contrast to the three-factor structure of the original SSD-12, the Bangla version indicated a single-factor structure (accounting for 61.29% of the total variance). This scale also demonstrates its ability to distinguish between clinical and non-clinical participants (t198 = 16.74, p < .01).

Conclusion

The Bangla translated SSD-12 has demonstrated strong psychometric properties, indicating its suitability for use in Bangladesh. This tool is expected to aid mental health practitioners in their clinical work by providing them with a quick assessment of their patients having somatic complaints.
Demographics and regional trends of ischemic heart disease-related mortality in older adults in the United States, 1999–2020
Authors: Latif Fakhar , Nasir Muhammad Moiz , Rehman Wajeeh Ur , Hamza Mohammed , Mattumpuram Jishanth , Meer Komail Khalid , Silvet Helme , Yarkoni Alon , Sabouni Mouhamed Amr , Braiteh Nabil , Patel Keyoor , Nashwan Abdulqadir J.
Published: January 2025

Background

Ischemic heart disease (IHD) has a significant impact on public health and healthcare expenditures in the United States (US).

Methods

We used data from the CDC WONDER database from 1999–2020 to identify trends in the IHD-related mortality of patients ≥ 75 years in the US. AAMRs per 100,000 population and APC were calculated and categorized by year, sex, race, and geographic divisions.

Results

Between 1999 and 2020, a total of 8,124,568 IHD-related deaths were recorded. Notable declines in AAMR were observed from 1999 to 2014 (APC: -3.86) and from 2014 to 2018 (APC: -2.55), with an overall increase from 2018 to 2020 (APC: 3.76). Older men consistently demonstrated higher AAMRs than older females, with AAMRs for both sexes decreasing steadily from 1999 to 2018 and increasing in 2020. When stratified by race/ethnicity, Whites (1931.7) had the highest AAMR, followed by Blacks (1836.5), American Indians (1510.5), Hispanics (1464.4), and Asians (1093.6). Furthermore, nonmetropolitan areas (2015.2) showed greater AAMRs than metropolitan areas (1841.8). The ≥ 85-year group consistently exhibited higher IHD-related mortality rates compared to the 75–84 years group. In comparison, the older group [≥75 years] (1873.0) consistently exhibited higher IHD-related AAMRs than the younger group [<75 years] (64.0) throughout the study, showing a significant disparity. Chronic IHD (1552.0) consistently showed the highest AAMRs throughout the study, surpassing myocardial infarction (515.6), other ischemic heart diseases (24.0), and angina pectoris (5.6).

Conclusion

Targeted interventions and resource allocation are crucial for areas with high IHD-related mortality. Public health policies should address demographic and geographical disparities, with further research for effective strategies.
Virtual reality perimetry compared to standard automated perimetry in adults with glaucoma: A systematic review
Authors: Hekmatjah Natan , Chibututu Chimelie , Han Ying , Keenan Jeremy D. , Oatts Julius T.
Published: January 2025

Purpose

The purpose of this systematic review was to consolidate and summarize available data comparing virtual reality perimetry (VRP) with standard automated perimetry (SAP) in adults with glaucoma. Understanding the utility and diagnostic performance of emerging VRP technology may expand access to visual field testing but requires evidence-based validation.

Methods

A systematic literature search was conducted in 3 databases (PubMed Central, Embase, and Cochrane Central Register of Controlled Trials) from the date of inception to 10/09/2024. Eligibility criteria included randomized controlled trials or prospective or retrospective cohort studies that compared different modalities of VRP to SAP in adults >18 years of age with glaucoma. Studies were excluded if they were review articles, letters, case reports, abstract-only papers, unavailable full text, or non-English language. Identified studies were formally evaluated for risk of bias using the Newcastle-Ottawa tool. The study protocol was prospectively registered with PROSPERO in May 2023 (registration number: CRD42023429071).

Results

The literature search yielded 1657 results. After deduplication, abstract and title screening, 14 studies met inclusion criteria and were included in the final systematic review. Compared to Humphrey Field Analyzer or Octopus 900, 10 different VRP devices were included in our study: Oculus Quest, Smartphone-based Campimetry, Toronto Portable Perimeter, VirtualEye, Advance Vision Analyzer, VisuALL, Vivid Vision Perimeter, C3 fields visual field analyzer, Radius, and Virtual Field. Overall, published studies of VRP are promising; however, more work is required to better evaluate these devices, namely test-retest repeatability.

Conclusions

VRP holds strong potential to evaluate visual fields in adults with glaucoma, though further data is needed to validate emerging technologies and testing protocols. Eye providers may consider using these devices to monitor certain adults with glaucoma.
Assessment of the bypass of obturation materials beyond fractured instruments after using different obturation techniques in simulated curved canals (An in-vitro study)
Authors: Lateef Ammar Aziz , Hameed Mohammed Rasheed , Fadhil Noor Hayder , Ali Ahmed Hamid
Published: January 2025

This study evaluated the extent to which obturation materials bypass fractured endodontic instruments positioned in the middle and apical thirds of severely curved simulated root canals using different obturation techniques. Sixty resin blocks with simulated root canals were used, each with a 50° curvature, a 6.5 mm radius of curvature, and a length of 16.5 mm, prepared to an ISO #15 diameter and taper. Canals were shaped using ProTaper Universal files (Dentsply Maillefer) attached to an X-smart Plus endo motor (Dentsply), set at 3.5 Ncm torque and 250 rpm, up to size S2 at working length. To simulate fractures, F2 and F3 files were weakened 3 mm from the tip, then twisted to break in the apical and middle sections of the canal, respectively. All samples were sealed with GuttaFlow 2 and divided into three groups (n = 20/group) according to obturation technique: A) single cone, B) lateral condensation with a rotary spreader, and C) softcore obturators. Each group was then divided into two subgroups (n = 10) based on the instrument fracture location (1 = apical, 2 = middle). The linear intrusion of obturation materials through the fractured instruments was measured using ImageJ software and analyzed statistically with ANOVA, Tukey tests, and independent t-tests, with significance set at p<0.05. Material bypass in group B1 (3.27 ± 0.63 mm) was significantly greater than in group A1 (2.39 ± 0.44 mm) and group C1 (2.91 ± 0.77 mm). In group C2, bypass (5.76 ± 0.64 mm) was significantly higher than in group A2 (3.82 ± 0.2 mm) and group B2 (2.27 ± 0.96 mm). Additionally, bypass in group A2 was greater than in group B2, and group B1 had more bypass than B2, while group C2 exceeded C1. The lateral condensation technique with a rotary spreader and softcore obturators increased the bypass of obturation materials through fractured instruments in simulated curved canals. These techniques may thus enhance material flow in endodontic procedures involving instrument fractures.
The etiology of attention deficit disorder with hyperactivity: A protocol for an umbrella review
Authors: Su Wenhao , Jia Hairong , Yang Luo , Zhang Jiaqi , Wei Zhaoyang , Tsikwa Pepertual , Wang Yanru
Published: January 2025

Introduction

Attention deficit hyperactivity disorder (ADHD) is one of the common neurodevelopmental disorders and is widely prevalent worldwide. The primary symptoms of ADHD include inattention, impulsivity, and hyperactivity, which significantly impact the cognitive, behavioral, and emotional dimensions of individuals. These disorders often continue throughout adulthood and, along with associated complications, affect various domains such as personal health, academic achievement, and social interactions. The pathogenesis and contributing causes of ADHD remain unclear at present. Therefore, this study aims to perform an umbrella review of systematic reviews and meta-analyses (SRMAs) to systematically assess the quality of methodologies, potential biases, and validity of all epidemiological evidence related to risk factors for ADHD while offering a comprehensive summary of the evidence regarding these risk factors.

Methods and analysis

This study follows the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) and Cochrane Handbook. We will systematically search 6 databases, including The Cochrane Library Central, PubMed, Embase, Web of Science, CINAHL, and Scopus from the initial period up until 2024 (last update). We will assess the quality of the included SRMAs using the tool to assess risk of bias in systematic reviews (ROBIS), the methodological quality of systematic reviews (AMSTAR)-2, PRISMA-2020, and the grade of recommendations assessment, development and evaluation (GRADE). Two authors will use the ecological models of health behavior to classify the causes and risk factors of ADHD. Finally, we will provide descriptive and comprehensive recommendations for clinical practice and future research.

Trial registration

PROSPERO (CRD42024597126).
Evolution of SARS-CoV-2 in white-tailed deer in Pennsylvania 2021–2024
Authors: Marques Andrew D. , Hogenauer Matthew , Bauer Natalie , Gibison Michelle , DeMarco Beatrice , Sherrill-Mix Scott , Merenstein Carter , Collman Ronald G. , Gagne Roderick B. , Bushman Frederic D.
Published: January 2025

SARS-CoV-2 continues to transmit and evolve in humans and animals. White-tailed deer (Odocoileus virginianus) have been previously identified as a zoonotic reservoir for SARS-CoV-2 with high rates of infection and probable spillback into humans. Here we report sampling 1,127 white-tailed deer (WTD) in Pennsylvania, and a genomic analysis of viral dynamics spanning 1,017 days between April 2021 and January 2024. To assess viral load and genotypes, RNA was isolated from retropharyngeal lymph nodes and analyzed using RT-qPCR and viral whole genome sequencing. Samples showed a 14.64% positivity rate by RT-qPCR. Analysis showed no association of SARS-CoV-2 prevalence with age, sex, or diagnosis with Chronic Wasting Disease. From the 165 SARS-CoV-2 positive WTD, we recovered 25 whole genome sequences and an additional 17 spike-targeted amplicon sequences. The viral variants identified included 17 Alpha, 11 Delta, and 14 Omicron. Alpha largely stopped circulating in humans around September 2021, but persisted in WTD as recently as March of 2023. Phylodynamic analysis of pooled genomic data from Pennsylvania documents at least 12 SARS-CoV-2 spillovers from humans into WTD, including a recent series of Omicron spillovers. Prevalence was higher in WTD in regions with crop coverage rather than forest, suggesting an association with proximity to humans. Analysis of seasonality showed increased prevalence in winter and spring. Multiple examples of recurrent mutations were identified associated with transmissions, suggesting WTD-specific evolutionary pressures. These data document ongoing infections in white-tailed deer, probable onward transmission in deer, and a remarkable rate of new spillovers from humans.
The Valentino syndrome
Authors: Ulrich Bertram
Published: January 2025

Rudolph Valentino was idolized by female audiences and dismissed by journalists and mostly White males as a new cinematic type quite apart from societal norms. Often playing minority ethnic lovers (excluding White minorities) on-screen with unique mannerisms and personality traits, he challenged White patriarchal America at a time when immigrants and women asserted themselves as a new force in society and in the economy. In public, his media interactions were prodigious and often contentious feeding into the burgeoning star-making system of Hollywood. His varying cinematic and public personae formed by women and men in the film industry and from his own unabashed self, influenced the cult of the male sex symbol and introduced a new discourse on ‘otherness’ both sexually and racially.
Bloodstained Narratives: The Giallo Film in Italy and Abroad, Matthew Edwards and Fernando Gabriel Pagnoni Berns (eds) (2023)
Authors: Waters Sandra
Published: January 2025

Review of: Bloodstained Narratives: The Giallo Film in Italy and Abroad, Matthew Edwards and Fernando Gabriel Pagnoni Berns (eds) (2023)

Jackson, MS: University Press of Mississippi, 208 pp.,

ISBN 978-1-49684-446-0, p/bk, $30
arte en función del diseño de moda
Authors: Ursúa-Astrain Raúl , Revilla Carrasco Alfonso
Published: January 2025

Este artículo aborda la problemática observada en el grado superior de diseño de moda Hacer Creativo de Zaragoza, donde los estudiantes mostraban dificultades para desarrollar diseños fundamentados en una investigación basada en referentes artísticos contemporáneos. Esta reflexión se realiza a través de un ABP que plantea la 'objetofilia' como base conceptual. A través de un recorrido experimental teórico-práctico, se analizan qué dificultades, beneficios y contrariedades surgen de la experimentación en la coexistencia del arte y la funcionalidad en un diseño. A través de este proyecto se crea e implementa por primera vez la asignatura optativa «El arte en función del diseño» en el cuarto curso del grado. Finalmente se plantea que la educación en moda debe considerar un enfoque basado en la adquisición de herramientas procedimentales y conceptuales que ayuden a estructurar un diseño, y así favorecer la creación de prendas con mayor coherencia estética e incluso funcional.
Investigar (en arte)
Authors: Lekerikabeaskoa Gaztañaga Amaia
Published: January 2025

La fundamentación de partida sobre la base del reconocimiento del saber específico del arte y su (potencialidad) de transmisión, conducen hacia las necesarias cuestiones de la investigación que el artículo trata de poner sobre la mesa, a raíz de diversos métodos y paradigmas existentes. Antecedentes contemporáneos que han tomado al arte como objetivo de (su) investigación, o han pretendido perfilar cauces para investigar (en) el arte como materia-prima. El cuerpo de contenidos discurre por los entresijos de las formas de investigar (no al uso) de cara a la creación, la enseñanza o la transmisión, poniendo el acento sobre las metodologías cuyas diferencias, pero también aspectos equivalentes, son susceptibles de subrayar. Finalmente, cómo se realizan los registros, validaciones y consiguientes ‘evaluaciones’ de dicha investigación son vertientes relevantes para su adecuación a los parámetros consensuados de verificación.
«Los ángeles exterminados»
Authors: Seijas Garzón Ane
Published: January 2025

El texto que se presenta tiene como base el caso de los Los ángeles exterminados (1968), serie de televisión en dos capítulos producida en el año 1968 por la Office de Radiodiffusion-Télévision Française (ORTF), filmada durante el segundo exilio de José Bergantín en París, no pudo emitirse en España hasta 1996. Planteamos de qué manera se relacionan el medio cultural, tendente a la estandarización, y otras formas de creación relacionadas con la percepción estética y espiritual de la realidad. En el caso que nos ocupa la visión sensible a la que nos referimos está determinada por el pensamiento de José Bergamín, quien escribió el guion de la mencionada serie. Es así que la noción de Arte será comprendida desde la polifacética y contradictoria visión del autor. Asimismo, deberá ser tenido en cuenta el que fuera responsable de dar imagen al guion, es decir, el director de la serie Michel Mitrani, quien comprometido con la transmisión del pensamiento bergaminiano propuso a la televisión francesa la creación de la serie.
Kraken Urbano Chileno o cómo el Estado Neoliberal creó una megarregión subsidiaria en el siglo XXI
Authors: Alvarado Peterson Voltaire , Hidalgo Dattwyler Rodrigo , Arenas Vásquez Federico , Pérez-Bustamante Leonel
Published: January 2025

La progresión espacial de la vivienda subsidiada en Valparaíso y Santiago se describe en este artículo desde la figura mítica del Kraken y su capacidad para ilustrar la forma de megarregión construida entre ambas metrópolis del centro de Chile. Impulsadas por la expansión de los proyectos habitacionales desde ambas ciudades, las políticas de subsidio existentes desde el retorno a la democracia en 1990 a la actualidad han marcado al territorio desde signos políticos distintos y adyacentes a la vez. De cierta forma, la convergencia de derechas e izquierdas no altera el resultado de la propiedad habitacional en tanto dispositivo de producción del bienestar social del neoliberalismo chileno. Siguiendo lo anterior, el eje del presente manuscrito está en las condiciones espaciales generadas por las políticas subsidiarias del denominado Estado Neoliberal, que en Chile ha sobrevivido entre colores opuestos en lo ideológico. Esto ha permitido la construcción de una estructura subsidiaria residencial eficaz además de ágil en actualizaciones y sofisticaciones normativas. El caso de estudio describe las dinámicas residenciales entre las metrópolis de Santiago y Valparaíso a partir de los datos intercensales entre 1992, 2002 y 2017, las que permiten relevar los impactos subsidiarios en la consolidación de esta nueva figura del Kraken Urbano chileno.
¿Por qué se vacía la Andalucía rural según sus pobladores? Análisis de los factores de abandono y permanencia desde una perspectiva cualitativa
Authors: Del Valle Ramos Carolina , Nieto Calmaestra José Antonio , Larrubia Vargas Remedios , Natera Rivas Juan José
Published: January 2025

Aunque Andalucía no destaca por ser una región muy afectada por la despoblación, lo cierto es que buen número de sus núcleos rurales presentan dinámicas demográficas regresivas. En este contexto, el trabajo que se presenta indaga en las motivaciones y percepciones que tienen los habitantes que residen en dichos ámbitos sobre el proceso de despoblación de sus pueblos, así como en los factores de atracción y abandono y en sus consecuencias. La presente investigación se ha realizado bajo una metodología cualitativa a partir del análisis de entrevistas semiestructuradas dirigidas a distintos perfiles de residentes en municipios afectados por la despoblación. Los resultados obtenidos ponen de manifiesto que el fenómeno de la despoblación es percibido por la población afectada como un hecho multicausal. Valoran positivamente sus territorios, pero muestran preocupación por el futuro y centran los motivos de dicha situación en factores económicos y laborales, que son contrarrestados por los valores de arraigo, el sentimiento de pertenencia al lugar y la familiaridad.
Classification of granitic relief in the territory of the Sertão Monumental Geopark Project, Brazil
Authors: Alcântara Alexandre Pinheiro , Claudino-Sales Vanda , Olímpio João Luis Sampaio , Simon Adriano Luís Heck , Maia Rubson Pinheiro
Published: January 2025

The granitic relief is notable in the Brazilian northeastern landscape due to its resistance to geomorphological processes. In the territory of the Sertão Monumental Geopark Project, which covers the Ceará municipalities of Quixadá and Quixeramobim, there is a significant occurrence of the granitic features, with different shapes and sizes showing subsurface and surface processes. The Etchplanation Theory and morphodynamic studies enabled the analysis of the development of these forms, which was conducted through a literature review, fieldwork and photographic records. The features were classified into macroforms and microforms, taking into account their characteristics and origin. Macroforms are extensive plutonic bodies that stand out in areas of planation relief, exhibiting diverse morphogenetic characteristics, with varying sizes, including inselgebirg, inselberg, flagstones and vertical outcrops. The microforms were subdivided into features associated with weathering, saprolitization and fracturing, and include tafoni, “honeycomb”, karren, gnammas and flared slopes. This classification and hierarchization contributes to future research and further study on the subject of granitic relief.
Mapeo colaborativo y análisis de la distribución geográfica de la agricultura urbana en Xalapa (Veracruz), México
Authors: Fontalvo-Buelvas Juan Camilo , Pulido Silva María Teresa , Escalona Aguilar Miguel Ángel , Falfán Ina
Published: January 2025

La agricultura urbana (AU) es un fenómeno complejo del cual pocas veces se conoce su geografía y que generalmente opera con huertos aislados, situaciones que dificultan la planificación estratégica de acciones de mejora. El objetivo del presente estudio se centró en mapear las iniciativas de huertos urbanos y analizar su distribución geográfica en el municipio de Xalapa (Veracruz), prototipo de la AU en México. La metodología toma como punto de partida el mapeo colaborativo mediante una plataforma de recolección de datos cartografiables (Ushahidi), que fueron validados, curados y manejados para su análisis en Quantum Geographic Information System (QGIS). Los resultados muestran el registro de 160 huertos para la Zona Metropolitana de Xalapa (ZMX), los cuales tienen asociada una comunidad aproximada de 3.263 personas y ocupan una superficie de 205.506 m2. La distribución espacial de la AU en el municipio tiende al agrupamiento y se concentró principalmente en la mancha intraurbana. La mayor parte de los huertos urbanos están ubicados en la ciudad de Xalapa-Enríquez, especialmente en colonias con baja y media marginación, sugiriendo que los huertos no están vinculados mayormente a la autosuficiencia alimentaria, sino a causas socioecológicas como reconstrucción del tejido social y la defensa del territorio. La propuesta metodológica puede ser replicable para aproximarnos al fenómeno de la AU en otras ciudades, así como para generar cartografías interactivas para la articulación de redes de colaboración y políticas públicas, aspectos cruciales para la sustentabilidad de la agricultura urbana.
Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.
Authors: Chuprom Julalak , Sangkanu Suthinee , Mitsuwan Watcharapong , Boonhok Rachasak , Paul Alok K. , Oliveira Sonia M. Rodrigues , Pereira Maria L. , Jimoh Tajudeen O. , Rahmatullah Mohammed , Wilairatana Polrat , Wiart Christophe , Verma Ajoy K. , Nissapatorn Veeranoot
Published: January 2025

Background and Aim: Acanthamoeba spp. are pathogenic microorganisms linked to severe infections in humans and animals, requiring a deeper understanding of their encystation process for effective diagnostics and research. This study focused on developing a novel encystment medium to induce synchronized encystation of Acanthamoeba spp. efficiently and rapidly.

Materials and Methods: The study employed response surface methodology with a central composite design to optimize the encystment medium formulation. The key components included Tris-HCl, NaCl, glucose, and MgCl2. The optimized liquid medium was spray-dried to produce a dehydrated powder for practical application. The encystation efficiency of different Acanthamoeba strains was assessed using hemocytometry and fluorescence microscopy.

Results: The optimized medium, comprising 3.152 g/L Tris-HCl, 5.55 g/L NaCl, 8% (w/v) glucose, and 5.0 mM MgCl2 at pH 9.0, demonstrated exceptional encystation efficiency with rates ranging from 99% to 100%. A spray-dried powdered version of this medium was equally effective, achieving a 98.77% encystation rate for A. castellanii American Type Culture Collection 50739 in glucose-free conditions. Notably, optimal glucose concentrations varied among Acanthamoeba strains, with certain strains reaching maximum encystation at 6–8% glucose.

Conclusion: This study successfully developed an innovative encystment medium that promotes rapid and efficient cyst production in Acanthamoeba spp. The medium enhances laboratory research and diagnostic capabilities, paving the way for future advancements in understanding and managing Acanthamoeba infections.

Keywords: Acanthamoeba, diagnostic tools, encystation, medium optimization, response surface methodology.
Conservação com inovação: o desenho animado “Mar à Vista!”
Authors: Almeida Luana , Azevedo Karoline , Villanova Maria , Malhado Ana Cláudia Mendes
Published: January 2025

A UNESCO recomenda que a educação ambiental seja contínua e comece na pré-escola, com uma abordagem interdisciplinar e conectada às experiências de vida das crianças. Com base nisso, desenvolvemos uma animação 2D ambientada na maior APA federal costeiro-marinha do Brasil, voltada para jovens de 2 a 12 anos, visando sensibilização ambiental e literacia oceânica. Com duas temporadas, a animação integra músicas autorais sobre temas ambientais, fortalecendo a identidade cultural. Com mais de 18 mil visualizações, a série foi reconhecida na imprensa local, participou de festivais de audiovisual e recebeu prêmios, como "Comprometidos com os oceanos e o meio ambiente", da UNESCO, Ashoka, Socialab e National Geographic. O projeto "Mar à Vista!" visa formar uma geração que respeite o ambiente, promovendo a preservação da biodiversidade e a valorização da cultura regional.
ACESSIBILIDADE PARA PESSOAS COM DEFICIÊNCIA VISUAL NOS ESPETÁCULOS DO GRUPO DE DANÇA DIVERSUS.
Authors: Dalla Déa Vanessa Helena Santana , Lima Marlini Dorneles de
Published: January 2025

O acesso à cultura é um direito das pessoas com deficiência visual que muitas vezes se torna impossível por falta de acessibilidade comunicacional nos espetáculos de dança. O Grupo de Dança Diversus da Universidade Federal de Goiás atua desde 2017 com foco em uma dança que atenda todas as pessoas com e sem deficiência. Vem refletindo, discutindo e realizando ações para que a dança seja acessível para os bailarinos e para as pessoas que assistem seus espetáculos. Esse artigo tem como objetivo apresentar as estratégias acessíveis realizadas em três espetáculos de dança do grupo para a inclusão da pessoa com deficiência visual enquanto plateia. Trata-se de uma pesquisa qualitativa, exploratória e descritiva documental que apresenta as estratégias acessíveis de três espetáculos do Grupo de Dança Diversus, sendo um presencial e dois vídeo-danças.  Espera-se com esse artigo dar visibilidade para possibilidades, dificuldades e caminhos encontrados pelo Grupo na busca pela acessibilidade comunicacional. A dança inclusiva pode ser uma oportunidade das pessoas com deficiência visual, tanto bailarinos quanto público, repensarem e ampliarem a inclusão social, o direito à diversidade e a dignidade humana.
Určení rozhodného práva pro odpovědnost vyplývající z užití díla v kyberprostoru
Authors: Čermák Jiří
Published: January 2025

The Article deals with applicability of the principle of territoriality, as introduced in the Berne Convention for the Protection of Literary and Artistic Works and new Regulation (EC) No 864/2007 on law applicable to non-contractual obligations (Rome II) to obligations arising from an infringement of copyright.
The recognized principle of lex loci protectionis, meaning the law of the country in which protection is claimed (on which e.g. the Bern Convention and Regulation Rome II are built), stipulates that the state shall apply its own law to an infringement of copyright infringement that is in force in its territory. This rule, also known as the “territoriality principle,” requests that the courts apply the law of the country where the violation (copyright infringement) was committed. This solution confirms that the rights held in each country are independent.
The key issue, however, is how the principle of territoriality shall be applied to copyright infringements committed through the Internet. An author of the article concludes that as a general rule, the principle of territoriality should be interpreted in a way that a copyright holder who complains that his work has been exploited without regard for his rights will be entitled to the protection given him by the copyright law in the country where the alleged infringement was committed.
In particular, in case of communication of copyrighted works to the public over the Internet, laws of all countries from which such disseminated work can be accessed may be applied in addition to the copyright laws of the jurisdiction where a server (on which the infringing content is stored) is located.
In case of downloading from the Internet, such act should be governed by copyright laws of a jurisdiction in which an end user (who initiates such download) or such end user’s computer is located.
Peer-to-peer sítě z hlediska trestního práva
Authors: Minárik Tomáš
Published: January 2025

The article describes the legal status of peer-to-peer filesharing in the Czech Republic, with a particular focus on criminal law. It touches on some contentious issues of the borderline between private and public law. Finally, it contains a brief overview of possible legal solutions to the filesharing problem.
K neoprávněnému podnikání v kybernetickém prostoru (aneb o internetových sázkových kancelářích v českém právu)
Authors: Patěk Daniel , Paťková Petra
Published: January 2025

The article deals with the activity of the foreign subjects providing on-line betting in the Czech Republic. It describes problems arising out the facility of internet betting crossing the borders and facing the restrictive attitude of the Czech legislation to these type of activities of foreign subjects. EU harmonization has not been reached in this field of business activity yet but each EU member state has to respect the general limits resulting from the decisions of European Court of Justice. Therefore Parliament of the Czech Republic is limited in the scale of possible restriction of on-line gambling. The article confronts these limits with the intention of senators to enacted an amendment of Lottery Act 1990.
Některé právní aspekty forenzní analýzy digitálních dat
Authors: Selinšek Liljana
Published: January 2025

The article is dealing with some legal aspects of digital forensics from the viewpoint of the countries where digital forensics is not (yet) often used investigation method, and digital evidences are not everyday evidence means. The questions discussed in the article should be answered at the beginning of the establishment the proper and legally accepted relation between digital forensics and law (the stress is, however, on criminal law, because the author is working in this field). The main goal of the article is to open some questions and to offer some possible answers. However, it has to be stressed that there is no universal answers on the questions about relation between digital forensics and law. While digital forensics is based on the similar principles and rules all around the world, the law is pretty much specific for almost each country. Besides, different levels of technical and informational development of the countries have to be taken into consideration. Even if questions from the article are already solved in some countries, they have just opened in the other ones, so it can be stated these questions are going to be relevant for some time.
Evaluation of sour-type pomegranate germplasm for field performance, fruit yield and anardana quality attributes
Authors: Kumar R , Berwal M K , Gora J S , Vijay R R S
Published: January 2025

Thirty-one sour-type pomegranate genotypes have been evaluated for growth, fruit yield, physico-chemical attributes and anardana quality in randomized complete block design under hot arid climate. Germplasm CIAH PG-1 had highest fruit number/plant 46.92 as well as fruit yield 12.40kg/plant, followed by Gul-e-Shah R. Pink (42.74 fruit number/plant and 10.98kg/plant) and Tujetis EC-104347 (42.75 fruit number/plant and 9.96kg/plant). The highest fruit weight 263.84g was also recorded in CIAH PG-1. The highest aril content (67.71%) was observed in CIAH PG-1 which was statistically at par with Khog and Bedana Seedless. The highest juice acidity was recorded in Tujetis EC-104347 (3.57%), statistically alike CIAH PG-1 (3.50%). The lowest dehydration ratio was observed in CIAH PG-A-3 (2.23), comparable to CIAH PG-A-5 (2.31). Maximum anardana recovery was reported in CIAH PG-1 (2.23kg/plant) which was followed by Tujetis EC-104347 (1.93kg/plant) and Gul-e-Shah Rose Pink (1.82kg/plant). The anardana acidity ranged from 4.63 to 7.99% and the promising germplasms with more than 7% acidity were AHPG H-2, Tujetis EC-104347, CIAH PG-1, Khog, Gul-e-Shah, IC-318712 and Tabest. Based on nine points hedonic scale, CIAH PG-1 received the highest overall acceptable sensory score of 8.67, followed by Tujetis EC-104347 and Gul-e-Shah R. Pink at 8.37 and 8.23 respectively. In conclusion, CIAH PG-1 demonstrated exceptional performance across various matrices, including field performance, anardana recovery, quality and sensory attributes, which establishes it asthe superior choice for quality anardana production. Tujetis EC-104347 and Gul-e-Shah R. Pink also observed suitable option for processing applications and valuable contributors to future breeding programs.
Evaluating the efficacy of tap water and commercial soap for outpatient wound management in simplified wound care
Authors: Jeong Hyung Hwa
Published: January 2025

Managing wounds outside of hospital settings can be challenging due to limited knowledge, resources, and financial constraints. This study evaluates the safety and efficacy of using tap water and a commercial soap-based cleanser for wound care in outpatient settings. This prospective study enrolled 15 patients with open or surgical wounds. Participants used a commercial soap containing surfactants (decyl glucoside, sodium C14- 16 olefin sulfate) and tap water to cleanse their wounds. Exclusion criteria included severe systemic illnesses, uncontrolled diabetes, and immunosuppression. We monitored wound healing, infection rates, and complications over a 2-week period. The study examined wounds of various origins: surgical (n=9), traumatic (n=4), and infection-related (n=2). All wounds healed completely within 2 weeks, with no instances of infection, dehiscence, or delayed healing observed. These findings indicate that a straightforward soap-and-water cleansing regimen is both safe and effective for wound management. This study demonstrates that using tap water and commercial soap is a cost-effective and reliable strategy for wound management outside hospital settings. This approach has the potential to simplify wound care practices and reduce barriers to treatment, particularly in resource-limited environments.
Mobile application-based monitoring in post-breast reconstruction surgery: current trends and future directions
Authors: Jeong Jae Hoon
Published: January 2025

Mobile apps are frequently utilized in various medical fields for postoperative care, yet there is limited research concerning their application among breast reconstruction patients. This paper examines the development of mobile apps for postoperative care and discusses key considerations for their design and implementation in the context of breast reconstruction patients. Mobile apps provide a holistic approach to recovery, featuring personalized recovery plans, wound monitoring, pain tracking, medication management, virtual consultations, and emotional support. These functionalities improve patient outcomes, facilitate communication between patients and healthcare providers, and enhance engagement with the recovery process. Advanced digital healthcare technologies are revolutionizing patient care, particularly in home settings, by improving monitoring capabilities and telemedicine, thus increasing access and efficiency. For mobile apps to be successfully adopted in postoperative care, it is crucial to address regulatory, financial, and engagement challenges to ensure they deliver sustained value to both patients and healthcare providers. Future research should aim to extend these solutions to plastic surgery and other medical disciplines to improve recovery outcomes for a broad range of patient populations.
Talking about sociolinguistic injustice: critical ethnographies towards speaker conscientization
Authors: Prego-Vázquez Gabriela , Molina Clara , Puigdevall Maite
Published: January 2025

This paper explores how researchers and co-participants in a joint Participatory Action Research (PAR) project in Galicia and Madrid discursively negotiate the conditions of possibility to co-construct spaces in which to talk about forms of sociolinguistic injustice. The aim of our multisite project is to create spaces for sociolinguistic research and intervention in which to identify the “sociodiscursive” elements used to manage the conditions of possibility that make it possible to address linguistic injustice. In our project we work with two related but not identical theoretical frameworks, Critical Language Awareness (CLA) and Freire’s pedagogical model of conscientization (or sensitization through discussion, reflection and action). Our findings reveal the presence of two types of conditions of possibility: “epistemic conditions of possibility”, derived from the participatory methodological framework itself, and “dialogic conditions of possibility”, that emerge in the participation framework of situated communicative activities. The data show how, in empathetic situations in which affect is verbalized, co-participants can reach what we call “eureka moments” when the naturalization of inequality is problematized. The newly-gained awareness of participants who reach these moments of revelation is evidenced in explicit manifestations in which people verbalize how they suddenly understand the why of past situations or realize that something may be beginning to change. In this paper we present the activities used in controlled environments to promote this shift, since awareness raising is something that cannot be carried out by the speakers themselves, at least not alone, and we discuss their effectiveness.
Redefining Creativity and Well-being: A Feasibility Study for a New Course at a Small Liberal Arts College in Japan
Authors: Friederich Lee , Taniguchi Yuko , Araki Naoko , Naganuma Naeko , Friederich Joel , Cullen Kathryn R.
Published: January 2025

Considering the escalating mental health needs of college students and the stigma surrounding mental illness in Japan, this study explores how creativity impacts student well-being. Eleven students enrolled in an intensive 2-week course participated in the study, completing the Comprehensive Inventory of Thriving (CIT) and post-course interviews. Total CIT scores increased significantly from pre- to post-course (p=0.04). Post-course interviews suggested eight themes, three of which are explored here: safe spaces, redefining creativity, and self-acceptance and self-compassion. The article includes a case study of one student’s learning journey to synthesize quantitative and qualitative findings. This preliminary study finds that creative activities, combined with learning in positive psychology, can help college-level students in Japan achieve a greater sense of well-being.
СТРАТЕГІЧНІ ОРІЄНТИРИ СІЛЬСЬКОГО ГОСПОДАРСТВА І СІЛЬСЬКИХ ТЕРИТОРІЙ УКРАЇНИ НА ПЕРІОД ДО 2030 р.: ВІДПОВІДНІСТЬ ЄВРОПЕЙСЬКОМУ ВИБОРУ
Authors: БОРОДІНА Олена , ПРОКОПА Ігор , ШУБРАВСЬКА Олена
Published: January 2025

З початком у 2024 р. офіційних переговорів щодо вступу України до ЄС перед державою постало надважливе завдання виконання умов, що висуваються європейською спільнотою до країн – претендентів на членство в Союзі. Однією з таких умов є приведення національних нормативно-правових актів, у тому числі в аграрній сфері, у відповідність з європейськими документами.
Європейський підхід до аграрного і сільського розвитку базується на багатофункціональності сільського господарства, що дістало відображення в Довгостроковому баченні сільських територій ЄС до 2040 року і низці інших програмних документів, прийнятих з метою забезпечення процвітання сільських територій Європи, її продовольчої й енергетичної безпеки. Він також передбачає розширення можливостей сільських громад, сприяння соціальним інноваціям, поліпшення доступу до послуг, особливо комунікаційних; збереження природних ресурсів і екологізацію сільського господарства, диверсифікацію й підвищення частки доданої вартості від економічної діяльності сільського населення.
Прийняття Україною Стратегії розвитку сільського господарства та сільських територій на період до 2030 року стане вагомим кроком до використання європейських цінностей у післявоєнному оновленні аграрного сектору. Разом з тим у ній нечітко визначено шляхи й способи розв’язання окремих проблем. Для їх уточнення обґрунтовано необхідність і напрями подолання структурних деформацій у сільському господарстві, зокрема законодавчого внормування агрохолдингів і застосування використовуваного в ЄС поняття «активний фермер»; підвищення продуктивності й конкурентоспроможності виробництва, доходів виробників, ефективності системи обміну знаннями та інноваціями; запровадження диференційованого підходу до підтримки різних груп сільгоспвиробників, повоєнного вдосконалення земельних відносин, чіткого виокремлення заходів з екологізації і кліматичної адаптації сільського господарства тощо.
УКРАЇНСЬКА ЕКОНОМІКА І ЕКОНОМІЧНА ПОЛІТИКА
Authors: ЩЕРБАКОВА Олена
Published: January 2025

Війни, що завжди є трагедією, часто стають каталізатором багатьох суспільних і економічних процесів. Повномасштабна війна теж виступила таким «рушієм» для дискусії стосовно економічної моделі України, яка дозволить вистояти в затяжному протистоянні з РФ і якою вона має стати після завершення війни. Вироблення дієвих рекомендацій щодо того, як пожвавити економічне зростання і яку роль у цьому процесі може відігравати центральний банк, потребує з’ясування того, яким чином змінювалась українська економіка після інтеграції у світову економічну систему, і в якому стані вона перебувала на початок війни й під час повномасштабної агресії.
Проаналізовано зміни в українській економіці за останні 15 років, на основі чого визначено окремі заходи для підтримки економічного зростання в умовах війни і роль у цьому процесі політики центрального банку. При дослідженні використано такі методи, як порівняння і співставлення, системний аналіз, дедукція і графічна інтерпретація. Проаналізовано зміни в структурі економіки України в динаміці – від моменту світової фінансової кризи 2008 р. і до 2021–2023 рр. (моменту останніх наявних даних). Вказано на існуючий ризик руху економічної політики за траєкторією «найменшого спротиву», який сьогодні може проявлятись у формальному виконанні лише необхідних для отримання  фінансової допомоги зобов’язань. Позитивом залишається те, що постійні кризи зробили український бізнес надзвичайно стійким до шоків і потрясінь, що дозволяє економічним агентам швидко адаптуватися до мінливих зовнішніх і внутрішніх умов. Та сама логіка є прийнятною і по відношенню до розробників економічної політики в Україні.