bluuebunny/crossref_metadata_2025 huggingface dataset:
A prospective study of sleep status, anxiety, and depression levels of college students at a university in Shandong Province, China
Authors: Zhang Chengshuai , Zhao Ling , Dong Tingting , Zhao Ji , Gao Cui , Zhao Feng
Published: April 2024

Objective

To investigate the changes in sleep conditions, anxiety, and depression levels among college students before and after entering the university.

Methods

Utilizing a random sampling method, 692 new students from a college in Shandong province were selected in September 2019, and relevant indices were statistically analyzed in September 2021 following a comprehensive follow-up. Sleep status, anxiety, and depression levels were assessed using the Pittsburgh Sleep Quality Index (PSQI), Patient Health Questionnaire-9 (PHQ-9), and Generalized Anxiety Disorder-7 (GAD-7), respectively.

Results

Gender, passive smoking, exercise, intake of fruits, and intake of seafood were identified as significant influencing factors on college students’ sleep status, anxiety, and depression levels (p < 0.05). A substantial difference was observed in the sleep quality of college students between the early enrollment stage and the follow-up stage (p < 0.05). Moreover, a significant positive correlation was found between PSQI scores and the levels of anxiety and depression (p < 0.05), cumulatively explaining approximately 10% of the variance in anxiety and depression levels.

Conclusion

The sleep quality of college students exhibited significant improvement after enrollment compared to the early enrollment period. Engaging in appropriate exercise and consuming fruits and seafood demonstrated a positive impact on sleep conditions, anxiety, and depression levels. These findings underscore the importance of fostering healthy lifestyle habits for promoting overall well-being among college students.
Exploring the impact of smartphone addiction on decision-making behavior in college students: an fNIRS study based on the Iowa Gambling Task
Authors: Liu Xiaolong , Tian Ruoyi , Bai Xue , Liu Huafang , Li Tongshu , Zhou Xinqi , Lei Yi
Published: April 2024

The pervasive use of smartphones, while enhancing accessibility to information and communication, has raised concerns about its potential negative effects on physical and mental health, including the impairment of decision-making abilities. This study investigates the influence of smartphone addiction on decision-making in college students. A sample of 80 individuals aged 17 to 26 was selected and divided into two groups based on their Smartphone Addiction Scale-Short Version (SAS-SV) scores. Participants underwent the Iowa Gambling Task (IGT) to evaluate their decision-making in risky and uncertain conditions, while fNIRS recorded their prefrontal cortex activity. The study found that individuals prone to smartphone addiction tend to make riskier choices in risky situations. However, when faced with decisions based on ambiguity, the smartphone addiction group showed increased brain activity in the dlPFC (specifically in channels 4, 9, and 11) compared to when making risky decisions. Despite this increased brain activation, there was no observable difference in behavior between the addiction-prone and control groups in ambiguous scenarios. Notably, the left dlPFC (e.g., channel 4) exhibited significantly higher activation in the addiction group compared to the control group. Findings suggest that smartphone addiction can detrimentally influence decision-making, behaviorally and neurologically, particularly in uncertain contexts. This study supports the classification of smartphone addiction as a genuine addiction and underscores its significance in psychiatric research. In essence, our research underscores the adverse effects of excessive smartphone use on decision-making processes, reinforcing the necessity to treat smartphone addiction as a pressing public health issue.
Protein hydrolysate and oil from fish waste reveal potential as dog food ingredients
Authors: Cabrita Ana R. J. , Maia Margarida R. G. , Alves Ana P. , Aires Tiago , Rosa Ana , Almeida André , Martins Rui , Fonseca António J. M.
Published: April 2024

The increased fish consumption by the growing human population in the world translates into an increase in fish waste. The reintroduction of these fish by-products into food and feed chains presents economic benefits and contributes to counteracting their negative environmental impact. Under this context, the present study aimed to evaluate the effects of the dietary inclusion of fish hydrolysate and oil obtained from fish waste (experimental diet) in substitution of shrimp hydrolysate and salmon oil (control diet) mainly imported from third countries on palatability, apparent total tract digestibility, fecal characteristics and metabolites, blood fatty acid profile, flatulence, and coat quality of adult dogs. A two-bowl test was performed to evaluate palatability by the pairwise comparison between the two diets. A feeding trial was conducted according to a crossover design with two diets (control and experimental diets), six adult Beagle dogs per diet, and two periods of 6 weeks each. The replacement of shrimp hydrolysate and salmon oil with fish hydrolysate and oil did not affect the first diet approach and taste, as well as the intake ratio. Generally, the digestibility of dry matter, nutrients, and energy was not affected by diet, but the intake of digestible crude protein (CP) and ether extract was higher, respectively, with the control and the experimental diet. The higher intake of eicosapentaenoic acid and docosahexaenoic acid with the experimental diet was reflected in a higher content of these long-chain polyunsaturated fatty acids and the omega-3 index of red blood cells, but it did not affect coat quality. The significantly higher intake of digestible CP with the control diet might have contributed to the higher fecal ammonia-N and valerate concentrations. Daily fecal output and characteristics were similar between diets. Overall, results suggest that fish hydrolysate and oil from the agrifood industry might constitute sustainable functional ingredients for dog feeding while adding value for wild fisheries, aquaculture, and fish farming under a circular economy approach and reducing dependence on imports from third countries with a high carbon footprint.
optimHome: A Shrinking Horizon Control Architecture for Bidirectional Smart Charging in Home Energy Management Systems
Authors: Caminiti Corrado Maria , Merlo Marco , Fotouhi Ghazvini Mohammad Ali , Edvinsson Jacob
Published: April 2024

This study aims to develop an adaptable home energy management system capable of integrating the bidirectional smart charging of electric vehicles. The final goal is to achieve a user-defined objectives such as cost minimization or maximizing renewable self-consumption. Industrialwise, the present work yields valuable outcomes in identifying operational frameworks and boundary conditions. Optimal scheduling benefits both users and the electric network, thus enhancing grid utilization and increasing renewable energy integration. By coordinating power interactions with dynamic time-of-use tariffs, the energy management system minimizes user costs and aids the grid by cutting peak hour energy consumption. Charging and discharging operations in electric vehicles comply with energy level constraints outlined by bidirectional charging protocols. The proposed approach ensures the scheduling of cycles that minimize detrimental effects on battery health when evaluating an economically ageing mechanism. Compared to uncontrolled charging, optimal scheduling resulted in a significant reduction in the total operational cost of the dwelling. Trade-off conditions between renewable integration and potential savings are identified and numerically evaluated by means of multiobjective optimization. In contrast to scheduling-based models, the proposed architecture possesses the ability to iteratively adapt decision variables in response to system changes, thus responding effectively to external stochastic uncertainty.
Enhancing Reliability in Floating Offshore Wind Turbines through Digital Twin Technology: A Comprehensive Review
Authors: Chen Bai-Qiao , Liu Kun , Yu Tongqiang , Li Ruoxuan
Published: April 2024

This comprehensive review explores the application and impact of digital twin (DT) technology in bolstering the reliability of Floating Offshore Wind Turbines (FOWTs) and their supporting platforms. Within the burgeoning domain of offshore wind energy, this study contextualises the need for heightened reliability measures in FOWTs and elucidates how DT technology serves as a transformative tool to address these concerns. Analysing the existing scholarly literature, the review encompasses insights into the historical reliability landscape, DT deployment methodologies, and their influence on FOWT structures. Findings underscore the pivotal role of DT technology in enhancing FOWT reliability through real-time monitoring and predictive maintenance strategies, resulting in improved operational efficiency and reduced downtime. Highlighting the significance of DT technology as a potent mechanism for fortifying FOWT reliability, the review emphasises its potential to foster a robust operational framework while acknowledging the necessity for continued research to address technical intricacies and regulatory considerations in its integration within offshore wind energy systems. Challenges and opportunities related to the integration of DT technology in FOWTs are thoroughly analysed, providing valuable insights into the role of DTs in optimising FOWT reliability and performance, thereby offering a foundation for future research and industry implementation.
Solution for Post-Mining Sites: Thermo-Economic Analysis of a Large-Scale Integrated Energy Storage System
Authors: Ochmann Jakub , Jurczyk Michał , Rusin Krzysztof , Rulik Sebastian , Bartela Łukasz , Uchman Wojciech
Published: April 2024

The intensive development of renewable energy sources and the decreasing efficiency of conventional energy sources are reducing the flexibility of the electric power system. It becomes necessary to develop energy storage systems that allow reducing the differences between generation and energy demand. This article presents a multivariant analysis of an adiabatic compressed air energy storage system. The system uses a post-mining shaft as a reservoir of compressed air and also as a location for the development of a heat storage tank. Consideration was given to the length of the discharge stage, which directly affects the capital expenditure and operating schedule of the system. The basis for the analyses was the in-house numerical model, which takes into account the variability of air parameters during system operation. The numerical model also includes calculations of Thermal Energy Storage’s transient performance. The energy efficiency of the system operating on a daily cycle varies from 67.9% to 70.3%. Various mechanisms for economic support of energy storage systems were analyzed. The levelized cost of storage varies, depending on the variant, from 75.86 EUR/MWh for the most favorable case to 223.24 EUR/MWh for the least favorable case.
Operational Robustness Assessment of the Hydro-Based Hybrid Generation System under Deep Uncertainties
Authors: Jiang Jianhua , Ming Bo , Huang Qiang , Bai Qingjun
Published: April 2024

The renewable-dominant hybrid generation systems (HGSs) are increasingly important to the electric power system worldwide. However, influenced by uncertain meteorological factors, the operational robustness of HGSs must be evaluated to inform the associated decision-making. Additionally, the main factors affecting the HGS’s robustness should be urgently identified under deep uncertainties, as this provides valuable guidance for HGS capacity configuration. In this paper, a multivariate stochastic simulation method is developed and used to generate uncertain resource scenarios of runoff, photovoltaic power, and wind power. Subsequently, a long-term stochastic optimization model of the HGS is employed to derive the optimal operating rules. Finally, these operating rules are used to simulate the long-term operation of an HGS, and the results are used to evaluate the HGS’s robustness and identify its main sensitivities. A clean energy base located in the Upper Yellow River Basin, China, is selected as a case study. The results show that the HGS achieves greater operational robustness than an individual hydropower system, and the robustness becomes weaker as the total capacity of photovoltaic and wind power increases. Additionally, the operational robustness of the HGS is found to be more sensitive to the total capacity than to the capacity ratio between photovoltaic and wind power.
Wind Turbine Tower State Reconstruction Method Based on the Corner Cut Recursion Algorithm
Authors: Liu Hongyue , Bai Yuxiang
Published: April 2024

This study introduces an innovative approach for the reconstruction of wind turbine tower states using a tangential recursion algorithm. The primary objective is to enable real-time monitoring of the operational condition of wind turbine towers. The proposed method is rooted in strain–load theory, which enables the accurate identification of tower load states. The tangential recursion algorithm is utilized to translate the strain data acquired from strategically placed sensors into reconstructed point positions. The subsequent refinement of these positions incorporates considerations of torsional loads and geometric deformations, culminating in the comprehensive and precise reconstruction of the tower’s deformation behavior. Through the use of the OpenFAST V8 simulation software, a thorough analysis is conducted to investigate the load and deformation characteristics of the NREL 5 MW wind turbine tower across diverse operational scenarios. Furthermore, the load conditions corresponding to rated operating circumstances are applied to a finite element model constructed with the lumped mass method. The identification of tower load states and the comprehensive reconstruction of deformation patterns are realized through the extraction of strain data from critical points in the finite element model. The credibility and accuracy of the proposed method are rigorously evaluated by juxtaposing the identification and reconstruction outcomes with the results derived from the OpenFAST simulations and finite element analyses. Notably, the proposed method circumvents the requirement for external auxiliary calibration equipment for the tower, rendering it adaptable to a broader spectrum of operational contexts and making it consistent with unfolding trajectories in wind power advancement.
“Damn the Empire!”: Imperial Excess, National Nostalgia, and Metaphysical Modernism in the Poetics of Parade’s End
Authors: Porter Molly Elizabeth
Published: April 2024

Ford Madox Ford famously intended his First World War tetralogy Parade’s End to have “for its purpose the obviating of all future wars”. But why do we engage in war to begin with? Modernist literature provides some provocative explanations. Ford’s Sylvia Tietjens, for example, proclaims that “You went to war when you desired to rape innumerable women. It was what war was for”. And in the very same year, Virginia Woolf’s shell-shocked Septimus Smith “went to France to save an England which consisted almost entirely of Shakespeare…” I argue that Ford’s understanding of the causality of war involves a strange combination of these two explanations in Parade’s End’s triangulation of seventeenth-century English literary tradition along with sexual and imperial conquest. While countless modernist novels exhibit a sensibility to the power of early modern poetry amidst battle, Parade’s End displays a particularly emphatic and extended focus on the relationship between poetic tradition and war. Soldiers of various ranks “talk…in intimate undertones about the resemblances between the Petrarchan and the Shakespearean sonnet form”, host timed sonnet competitions in the trenches, recurringly quote the seduction poetry of Marvell, and fantasize about George Herbert’s lifespan being “the only satisfactory age in England…yet what chance had it today? Or, still more, to-morrow?”. To answer this question, my own transtemporal study will use early modern scholarship to investigate seventeenth-century metaphysical poetry’s dual power to inspire and potentially obviate war. Much has been written on this tetralogy’s anti-linear plot but less on the broader temporality of its politico-literary vision. I contend that the metaphysical allusions of this text help Ford to show us the complexities of nationalism in the imperial conquest and imperial damnation that (early) modern aesthetics can catalyse.
Imbalance between Actin Isoforms Contributes to Tumour Progression in Taxol-Resistant Triple-Negative Breast Cancer Cells
Authors: Dugina Vera , Vasileva Maria , Khromova Natalia , Vinokurova Svetlana , Shagieva Galina , Mikheeva Ekaterina , Galembikova Aigul , Dunaev Pavel , Kudlay Dmitry , Boichuk Sergei , Kopnin Pavel
Published: April 2024

The widespread occurrence of breast cancer and its propensity to develop drug resistance highlight the need for a comprehensive understanding of the molecular mechanisms involved. This study investigates the intricate pathways associated with secondary resistance to taxol in triple-negative breast cancer (TNBC) cells, with a particular focus on the changes observed in the cytoplasmic actin isoforms. By studying a taxol-resistant TNBC cell line, we revealed a shift between actin isoforms towards γ-actin predominance, accompanied by increased motility and invasive properties. This was associated with altered tubulin isotype expression and reorganisation of the microtubule system. In addition, we have shown that taxol-resistant TNBC cells underwent epithelial-to-mesenchymal transition (EMT), as evidenced by Twist1-mediated downregulation of E-cadherin expression and increased nuclear translocation of β-catenin. The RNA profiling analysis revealed that taxol-resistant cells exhibited significantly increased positive regulation of cell migration, hormone response, cell–substrate adhesion, and actin filament-based processes compared with naïve TNBC cells. Notably, taxol-resistant cells exhibited a reduced proliferation rate, which was associated with an increased invasiveness in vitro and in vivo, revealing a complex interplay between proliferative and metastatic potential. This study suggests that prolonged exposure to taxol and acquisition of taxol resistance may lead to pro-metastatic changes in the TNBC cell line.
Redox Homeostasis Alteration Is Restored through Melatonin Treatment in COVID-19 Patients: A Preliminary Study
Authors: Soto María Elena , Pérez-Torres Israel , Manzano-Pech Linaloe , Palacios-Chavarría Adrían , Valdez-Vázquez Rafael Ricardo , Guarner-Lans Verónica , Soria-Castro Elizabeth , Díaz-Díaz Eulises , Castrejón-Tellez Vicente
Published: April 2024

Type II pneumocytes are the target of the SARS-CoV-2 virus, which alters their redox homeostasis to increase reactive oxygen species (ROS). Melatonin (MT) has antioxidant proprieties and protects mitochondrial function. In this study, we evaluated whether treatment with MT compensated for the redox homeostasis alteration in serum from COVID-19 patients. We determined oxidative stress (OS) markers such as carbonyls, glutathione (GSH), total antioxidant capacity (TAC), thiols, nitrites (NO2−), lipid peroxidation (LPO), and thiol groups in serum. We also studied the enzymatic activities of glutathione peroxidase (GPx), glutathione-S-transferase (GST), reductase (GR), thioredoxin reductase (TrxR), extracellular superoxide dismutase (ecSOD) and peroxidases. There were significant increases in LPO and carbonyl quantities (p ≤ 0.03) and decreases in TAC and the quantities of NO2−, thiols, and GSH (p < 0.001) in COVID-19 patients. The activities of the antioxidant enzymes such as ecSOD, TrxR, GPx, GST, GR, and peroxidases were decreased (p ≤ 0.04) after the MT treatment. The treatment with MT favored the activity of the antioxidant enzymes that contributed to an increase in TAC and restored the lost redox homeostasis. MT also modulated glucose homeostasis, functioning as a glycolytic agent, and inhibited the Warburg effect. Thus, MT restores the redox homeostasis that is altered in COVID-19 patients and can be used as adjuvant therapy in SARS-CoV-2 infection.
IQGAP3 Is an Important Mediator of Skin Inflammatory Diseases
Authors: Zolotarenko Alena , Bruskin Sergey
Published: April 2024

IQGAP3 (IQ Motif Containing GTPase Activating Protein 3) is member of the IQGAP family of scaffold proteins, which are essential for assembling multiprotein complexes that coordinate various intracellular signaling pathways. Previous research has shown that IQGAP3 is overexpressed in psoriatic skin lesions. Given its involvement in processes like cell proliferation and chemokine signaling, we sought to explore its molecular role in driving the psoriatic phenotype of keratinocytes. By conducting transcriptome profiling of HaCaT keratinocytes, we identified numerous psoriasis-associated pathways that were affected when IQGAP3 was knocked down. These included alterations in NFkB signaling, EGFR signaling, activation of p38/MAPK and ERK1/ERK2, lipid metabolism, cytokine production, and the response to inflammatory cytokine stimulation. Real-time analysis further revealed changes in cell growth dynamics, including proliferation and wound healing. The balance between cell proliferation and apoptosis was altered, as were skin barrier functions and the production of IL-6 and IFNγ. Despite these significant findings, the diversity of the alterations observed in the knockdown cells led us to conclude that IQGAP3 may not be the best target for the therapeutic inhibition to normalize the phenotype of keratinocytes in psoriasis.
Syndecans, Exostosins and Sulfotransferases as Potential Synovial Inflammation Moderators in Patients with Hip Osteoarthritis
Authors: Rošin Matko , Kelam Nela , Jurić Ivana , Racetin Anita , Ogorevc Marin , Corre Brieuc , Čarić Davor , Filipović Natalija , Vukojević Katarina
Published: April 2024

The gradual deterioration of articular cartilage was thought to be the central event in osteoarthritis (OA), but recent studies demonstrated the importance of low-grade synovitis in the progression of OA. The Syndecan (SDC) family of membrane proteoglycans is known to be involved in the regulation of inflammation, but there is limited evidence considering the role of syndecans in OA synovitis. Our study aimed to investigate the hip OA synovial membrane expression patterns of SDC1, SDC2 and SDC4, as well as exostosins and sulfotransferases (enzymes involved in the polymerisation and modification of syndecans’ heparan sulphate chains). Synovial membrane samples of patients with OA (24) were divided into two groups according to their Krenn synovitis score severity. The immunohistochemical expressions of SDC1, SDC2, SDC4, EXT1, EXT2, NDST1 and NDST2 in synovial intima and subintima were then analysed and compared with the control group (patients with femoral neck fracture). According to our study, the immunoexpression of SDC1, NDST1 and EXT2 is significantly increased in the intimal cells of OA synovial membrane in patients with lower histological synovitis scores and SDC4 in patients with higher synovitis scores, in comparison with non-OA controls. The difference in the expression of SDC2 among the OA and non-OA groups was insignificant. SDC1, SDC4, NDST1 and EXT2 seem to be involved as inflammation moderators in low-grade OA synovitis and, therefore, should be further investigated as potential markers of disease progression and therapeutic goals.
Change in Tissue Microbiome and Related Human Beta Defensin Levels Induced by Antibiotic Use in Bladder Carcinoma
Authors: Monyók Ádám , Mansour Bassel , Vadnay István , Makra Nóra , Dunai Zsuzsanna , Nemes-Nikodém Éva , Stercz Balázs , Szabó Dóra , Ostorházi Eszter
Published: April 2024

It is now generally accepted that the success of antitumor therapy can be impaired by concurrent antibiotic therapy, the presence of certain bacteria, and elevated defensin levels around the tumor tissue. The aim of our current investigation was to identify the underlying changes in microbiome and defensin levels in the tumor tissue induced by different antibiotics, as well as the duration of this modification. The microbiome of the tumor tissues was significantly different from that of healthy volunteers. Comparing only the tumor samples, no significant difference was confirmed between the untreated group and the group treated with antibiotics more than 3 months earlier. However, antibiotic treatment within 3 months of analysis resulted in a significantly modified microbiome composition. Irrespective of whether Fosfomycin, Fluoroquinolone or Beta-lactam treatment was used, the abundance of Bacteroides decreased, and Staphylococcus abundance increased. Large amounts of the genus Acinetobacter were observed in the Fluoroquinolone-treated group. Regardless of the antibiotic treatment, hBD1 expression of the tumor cells consistently doubled. The increase in hBD2 and hBD3 expression was the highest in the Beta-lactam treated group. Apparently, antibiotic treatment within 3 months of sample analysis induced microbiome changes and defensin expression levels, depending on the identity of the applied antibiotic.
EspP2 Regulates the Adhesion of Glaesserella parasuis via Rap1 Signaling Pathway
Authors: Tang Xinwei , Xu Shiyu , Yang Zhen , Wang Kang , Dai Ke , Zhang Yiwen , Hu Bangdi , Wang Yu , Cao Sanjie , Huang Xiaobo , Yan Qigui , Wu Rui , Zhao Qin , Du Senyan , Wen Xintian , Wen Yiping
Published: April 2024

Different levels of EspP2 expression are seen in strains of Glaesserella parasuis with high and low pathogenicity. As a potential virulence factor for G. parasuis, the pathogenic mechanism of EspP2 in infection of host cells is not clear. To begin to elucidate the effect of EspP2 on virulence, we used G. parasuis SC1401 in its wild-type form and SC1401, which was made EspP2-deficient. We demonstrated that EspP2 causes up-regulation of claudin-1 and occludin expression, thereby promoting the adhesion of G. parasuis to host cells; EspP2-deficiency resulted in significantly reduced adhesion of G. parasuis to cells. Transcriptome sequencing analysis of EspP2-treated PK15 cells revealed that the Rap1 signaling pathway is stimulated by EspP2. Blocking this pathway diminished occludin expression and adhesion. These results indicated that EspP2 regulates the adhesion of Glaesserella parasuis via Rap1 signaling pathway.
APOE ε2-Carriers Are Associated with an Increased Risk of Primary Angle-Closure Glaucoma in Patients of Saudi Origin
Authors: Kondkar Altaf A. , Azad Taif A. , Sultan Tahira , Khatlani Tanvir , Alshehri Abdulaziz A. , Radhakrishnan Rakesh , Lobo Glenn P. , Alsirhy Ehab , Almobarak Faisal A. , Osman Essam A. , Al-Obeidan Saleh A.
Published: April 2024

This study investigated the association between apolipoprotein E (APOE) gene polymorphisms (rs429358 and rs7412) and primary angle-closure glaucoma (PACG) and pseudoexfoliation glaucoma (PXG) in a Saudi cohort. Genotyping of 437 DNA samples (251 controls, 92 PACG, 94 PXG) was conducted using PCR-based Sanger sequencing. The results showed no significant differences in the allele and genotype frequencies of rs429358 and rs7412 between the PACG/PXG cases and controls. Haplotype analysis revealed ε3 as predominant, followed by ε4 and ε2 alleles, with no significant variance in PACG/PXG. However, APOE genotype analysis indicated a significant association between ε2-carriers and PACG (odds ratio = 4.82, 95% CI 1.52–15.26, p = 0.007), whereas no notable association was observed with PXG. Logistic regression confirmed ε2-carriers as a significant predictor for PACG (p = 0.008), while age emerged as significant for PXG (p < 0.001). These findings suggest a potential role of ε2-carriers in PACG risk within the Saudi cohort. Further validation and larger-scale investigations are essential to elucidate the precise role of APOE in PACG pathogenesis and progression.
Comparative Analysis of Gut Bacterial Community Composition in Two Tropical Economic Sea Cucumbers under Different Seasons of Artificial Environment
Authors: Jia Chenghao , Wang Yuanhang , Zheng Bojun , Wang Yanan , He Linwen , Xu Qiang , Gao Fei
Published: April 2024

With the continuous rise of the sea cucumber aquaculture industry in China, the tropical sea cucumber aquaculture industry is also improving. However, research on the gut microorganisms of tropical sea cucumbers in captivity is scarce. In this study, high-throughput sequencing methods were used to analyze the gut microbial composition of Stichopus monotuberculatus and Holothuria scabra in the dry season and wet season of artificial environments. The results showed that 66 phyla were obtained in all samples, of which 59 phyla were obtained in the dry season, and 45 phyla were obtained in the wet season. The Tax4Fun analysis showed that certain gut bacterial communities affect the daily metabolism of two sea cucumber species and are involved in maintaining gut microecological balance in the gut of two sea cucumber species. In addition, compared with differences between species, PCoA and UPGMA clustering analysis showed the gut prokaryotes of the same sea cucumber species varied more in different seasons, indicating that the influence of environment was higher than the feeding choices of sea cucumbers under relatively closed conditions. These results revealed the gut bacterial community composition of S. monotuberculatus and H. scabra and the differences in gut bacterial structure between two sea cucumber species in different seasons were compared, which would provide the foundation for tropical sea cucumber aquaculture in the future.
The Role of the Placental Enzyme Indoleamine 2,3-Dioxygenase in Normal and Abnormal Human Pregnancy
Authors: Kudo Yoshiki , Sugimoto Jun
Published: April 2024

The biologically significant phenomenon that the fetus can survive immune attacks from the mother has been demonstrated in mammals. The survival mechanism depends on the fetus and placenta actively defending themselves against attacks by maternal T cells, achieved through the localized depletion of the amino acid L-tryptophan by an enzyme called indoleamine 2,3-dioxygenase. These findings were entirely unexpected and pose important questions regarding diseases related to human pregnancy and their prevention during human pregnancy. Specifically, the role of this mechanism, as discovered in mice, in humans remains unknown, as does the extent to which impaired activation of this process contributes to major clinical diseases in humans. We have, thus, elucidated several key aspects of this enzyme expressed in the human placenta both in normal and abnormal human pregnancy. The questions addressed in this brief review are as follows: (1) localization and characteristics of human placental indoleamine 2,3-dioxygenas; (2) overall tryptophan catabolism in human pregnancy and a comparison of indoleamine 2,3-dioxygenase expression levels between normal and pre-eclamptic pregnancy; (3) controlling trophoblast invasion by indoleamine 2,3-dioxygenase and its relation to the pathogenesis of placenta accrete spectrum.
Characterization of Three Polysaccharide-Based Hydrogels Derived from Laminaria japonica and Their Hemostatic Properties
Authors: Chen Yang , Shi Jinying , Qiu Huamai , You Lijun , Xu Panqi , Rao Rui , Wu Minqian , Jia Ruohan
Published: April 2024

Three Laminaria japonica polysaccharides (LJPs) extracted via water extraction (LJP-W), acid extraction (LJP-A), and enzymatic extraction (LJP-E) were used as raw materials to be cross-linked with chitosan and polyvinyl alcohol to prepare hydrogels. Compared with conventional hydrogel systems, all three types of LJP-based polysaccharide hydrogels exhibited better swelling properties (14 times their original weight) and the absorption ability of simulated body fluid (first 2 h: 6–10%). They also demonstrated better rigidity and mechanical strength. Young’s modulus of LJP-E was 4 times that of the blank. In terms of hemostatic properties, all three polysaccharide hydrogels did not show significant cytotoxic and hemolytic properties. The enzyme- and acid-extracted hydrogels (LJP-Gel-A and LJP-Gel-E) demonstrated better whole-blood coagulant ability compared with the water-extracted hydrogel (LJP-Gel-W), as evidenced by the whole blood coagulation index being half that of LJP-Gel-W. Additionally, the lactate dehydrogenase viabilities of LJP-Gel-A and LJP-Gel-E were significantly higher, at about four and three times those of water extraction, respectively. The above results suggested that LJP-Gel-A and LJP-Gel-E exhibited better blood coagulation capabilities than LJP-Gel-W, due to their enhanced platelet enrichment and adhesion properties. Consequently, these hydrogels are more conducive to promoting coagulation and have good potential for wound hemostasis.
Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules
Authors: Lee So-Jeong , Kim Dong-Myung
Published: April 2024

The increasing demand for sustainable alternatives underscores the critical need for a shift away from traditional hydrocarbon-dependent processes. In this landscape, biomanufacturing emerges as a compelling solution, offering a pathway to produce essential chemical materials with significantly reduced environmental impacts. By utilizing engineered microorganisms and biomass as raw materials, biomanufacturing seeks to achieve a carbon-neutral footprint, effectively counteracting the carbon dioxide emissions associated with fossil fuel use. The efficiency and specificity of biocatalysts further contribute to lowering energy consumption and enhancing the sustainability of the production process. Within this context, cell-free synthesis emerges as a promising approach to accelerate the shift towards biomanufacturing. Operating with cellular machinery in a controlled environment, cell-free synthesis offers multiple advantages: it enables the rapid evaluation of biosynthetic pathways and optimization of the conditions for the synthesis of specific chemicals. It also holds potential as an on-demand platform for the production of personalized and specialized products. This review explores recent progress in cell-free synthesis, highlighting its potential to expedite the transformation of chemical processes into more sustainable biomanufacturing practices. We discuss how cell-free techniques not only accelerate the development of new bioproducts but also broaden the horizons for sustainable chemical production. Additionally, we address the challenges of scaling these technologies for commercial use and ensuring their affordability, which are critical for cell-free systems to meet the future demands of industries and fully realize their potential.
CB1 Receptor Negative Allosteric Modulators as a Potential Tool to Reverse Cannabinoid Toxicity
Authors: Flavin Audrey , Azizi Paniz , Murataeva Natalia , Yust Kyle , Du Wenwen , Ross Ruth , Greig Iain , Nguyen Thuy , Zhang Yanan , Mackie Ken , Straiker Alex
Published: April 2024

While the opioid crisis has justifiably occupied news headlines, emergency rooms are seeing many thousands of visits for another cause: cannabinoid toxicity. This is partly due to the spread of cheap and extremely potent synthetic cannabinoids that can cause serious neurological and cardiovascular complications—and deaths—every year. While an opioid overdose can be reversed by naloxone, there is no analogous treatment for cannabis toxicity. Without an antidote, doctors rely on sedatives, with their own risks, or ‘waiting it out’ to treat these patients. We have shown that the canonical synthetic ‘designer’ cannabinoids are highly potent CB1 receptor agonists and, as a result, competitive antagonists may struggle to rapidly reverse an overdose due to synthetic cannabinoids. Negative allosteric modulators (NAMs) have the potential to attenuate the effects of synthetic cannabinoids without having to directly compete for binding. We tested a group of CB1 NAMs for their ability to reverse the effects of the canonical synthetic designer cannabinoid JWH018 in vitro in a neuronal model of endogenous cannabinoid signaling and also in vivo. We tested ABD1085, RTICBM189, and PSNCBAM1 in autaptic hippocampal neurons that endogenously express a retrograde CB1-dependent circuit that inhibits neurotransmission. We found that all of these compounds blocked/reversed JWH018, though some proved more potent than others. We then tested whether these compounds could block the effects of JWH018 in vivo, using a test of nociception in mice. We found that only two of these compounds—RTICBM189 and PSNCBAM1—blocked JWH018 when applied in advance. The in vitro potency of a compound did not predict its in vivo potency. PSNCBAM1 proved to be the more potent of the compounds and also reversed the effects of JWH018 when applied afterward, a condition that more closely mimics an overdose situation. Lastly, we found that PSNCBAM1 did not elicit withdrawal after chronic JWH018 treatment. In summary, CB1 NAMs can, in principle, reverse the effects of the canonical synthetic designer cannabinoid JWH018 both in vitro and in vivo, without inducing withdrawal. These findings suggest a novel pharmacological approach to at last provide a tool to counter cannabinoid toxicity.
Beneficial Effects of Dietary Fiber in Young Barley Leaf on Gut Microbiota and Immunity in Mice
Authors: Chudan Seita , Kurakawa Takuto , Nishikawa Miyu , Nagai Yoshinori , Tabuchi Yoshiaki , Ikushiro Shinichi , Furusawa Yukihiro
Published: April 2024

The health benefits of young barley leaves, rich in dietary fiber, have been studied for several decades; however, their beneficial effects on the intestinal microenvironment remain to be elucidated. To investigate the effects of young barley leaf-derived dietary fiber (YB) on the gut microbiota and immunity, mice were fed an AIN-93G diet containing cellulose or YB and subjected to subsequent analysis. The population of MHC-II-positive conventional dendritic cells (cDCs) and CD86 expression in the cDCs of Peyer’s patches were elevated in the YB-fed mice. MHC-II and CD86 expression was also elevated in the bone marrow-derived DCs treated with YB. 16S-based metagenomic analysis revealed that the gut microbiota composition was markedly altered by YB feeding. Among the gut microbiota, Lachnospiraceae, mainly comprising butyrate-producing NK4A136 spp., were overrepresented in the YB-fed mice. In fact, fecal butyrate concentration was also augmented in the YB-fed mice, which coincided with increased retinaldehyde dehydrogenase (RALDH) activity in the CD103+ cDCs of the mesenteric lymph nodes. Consistent with elevated RALDH activity, the population of colonic IgA+ plasma cells was higher in the YB-fed mice than in the parental control mice. In conclusion, YB has beneficial effects on the gut microbiota and intestinal immune system.
Room Temperature Diels–Alder Reactions of 4-Vinylimidazoles
Authors: Fulton Brandon B. , Hartzell Alexia J. , Dias H. V. Rasika , Lovely Carl J.
Published: April 2024

In the course of studying Diels–Alder reactions of 4-vinylimidazoles with N-phenylmaleimide, it was discovered that they engage in cycloaddition at room temperature to give high yields of the initial cycloadduct as a single stereoisomer. In certain cases, the product precipitated out of the reaction mixture and could be isolated by simple filtration, thereby avoiding issues with aromatization observed during chromatographic purification. Given these results, intramolecular variants using doubly activated dienophiles were also investigated at room temperature. Amides underwent cycloaddition at room temperature in modest yields, but the initial adducts were not isolable with Nimid-benzyl-protected systems. Attempts to extend these results to the corresponding esters and hydroxamate were less successful with these substrates only undergoing cycloaddition at elevated temperatures in lower yields. Density functional theory calculations were performed to evaluate the putative transition states for both the inter- and intramolecular variants to rationalize experimental observations.
Synthesis, Spectroscopic Properties, and Metalation of 3-Alkoxybenziporphyrins
Authors: Tomlovich Rachel A. , Lash Timothy D.
Published: April 2024

A series of 5-alkoxy-1,3-benzenedicarbaldehydes and related dimers were prepared in three steps from dimethyl 5-hydroxyisophthalate. Acid catalyzed condensation of the dialdehydes with a tripyrrane dicarboxylic acid, followed by oxidation with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone, afforded good yields of 3-alkoxybenziporphyrins, although dimeric tetraaldehydes failed to give isolatable porphyrinoid products. Proton NMR spectroscopy gave no indication of an aromatic ring current, but addition of trifluoroacetic acid resulted in the formation of dications that exhibited weakly diatropic characteristics. Spectroscopic titration with TFA demonstrated that stepwise protonation took place, generating monocationic and dicationic species. 3-Alkoxybenziporphyrins reacted with nickel(II) or palladium(II) acetate to give the related nickel(II) or palladium(II) complexes. These stable organometallic derivatives showed increased diatropic properties that were most pronounced for the palladium(II) complexes. These unique porphyrinoids provide further insights into the properties of benziporphyrins.
Atypical Teratoid/Rhabdoid Tumor with Retained SMARCB1 (INI1) Expression and Rare SMARCA4 Gene Mutation: A Case Report of a Pediatric Patient
Authors: Mališkina Anna Marija , Franckeviča Ivanda , Višņevska-Preciniece Zelma , Grūtupa Marika , Kovaļova Žanna
Published: April 2024

Atypical teratoid/rhabdoid tumors (AT/RT) are highly aggressive tumors of the central nervous system (CNS), accounting for 1–3% of all pediatric CNS tumors. In general, AT/RTs are associated with biallelic inactivation of SMARCB1, resulting in the loss of expression of the integrase interactor 1 (INI1) protein. In this report, we describe the clinical course of an infant patient who presented with fatigue, postprandial vomiting, and disability of left side movement. Histological examination revealed classical features indicative of rhabdoid tumors, yet an atypical immunohistochemical profile with preserved INI1 expression was observed. Molecular diagnostics further elucidated the presence of a heterozygous frameshift variant, SMARCA4 c.2693del, p.(Asn898Thrfs*12), underscoring the distinctive genetic foundations of the case. Surgical resection of the tumor was administered with subsequent chemotherapy to the patient, but the condition worsened dynamically, and a decision was made to give the patient palliative therapy. We report on a patient with AT/RT caused by a rare mutation of the SMARCA4 gene and an aggressive course of disease to provide more information and characteristics of these tumors.
Investigation of the Efficacy of a Listeria monocytogenes Biosensor Using Chicken Broth Samples
Authors: Zolti Or , Suganthan Baviththira , Nagdeve Sanket Naresh , Maynard Ryan , Locklin Jason , Ramasamy Ramaraja P.
Published: April 2024

Foodborne pathogens are microbes present in food that cause serious illness when the contaminated food is consumed. Among these pathogens, Listeria monocytogenes is one of the most serious bacterial pathogens, and causes severe illness. The techniques currently used for L. monocytogenes detection are based on common molecular biology tools that are not easy to implement for field use in food production and distribution facilities. This work focuses on the efficacy of an electrochemical biosensor in detecting L. monocytogenes in chicken broth. The sensor is based on a nanostructured electrode modified with a bacteriophage as a bioreceptor which selectively detects L. monocytogenes using electrochemical impedance spectroscopy. The biosensing platform was able to reach a limit of detection of 55 CFU/mL in 1× PBS buffer and 10 CFU/mL in 1% diluted chicken broth. The biosensor demonstrated 83–98% recovery rates in buffer and 87–96% in chicken broth.
RobEns: Robust Ensemble Adversarial Machine Learning Framework for Securing IoT Traffic
Authors: Alkadi Sarah , Al-Ahmadi Saad , Ben Ismail Mohamed Maher
Published: April 2024

Recently, Machine Learning (ML)-based solutions have been widely adopted to tackle the wide range of security challenges that have affected the progress of the Internet of Things (IoT) in various domains. Despite the reported promising results, the ML-based Intrusion Detection System (IDS) proved to be vulnerable to adversarial examples, which pose an increasing threat. In fact, attackers employ Adversarial Machine Learning (AML) to cause severe performance degradation and thereby evade detection systems. This promoted the need for reliable defense strategies to handle performance and ensure secure networks. This work introduces RobEns, a robust ensemble framework that aims at: (i) exploiting state-of-the-art ML-based models alongside ensemble models for IDSs in the IoT network; (ii) investigating the impact of evasion AML attacks against the provided models within a black-box scenario; and (iii) evaluating the robustness of the considered models after deploying relevant defense methods. In particular, four typical AML attacks are considered to investigate six ML-based IDSs using three benchmarking datasets. Moreover, multi-class classification scenarios are designed to assess the performance of each attack type. The experiments indicated a drastic drop in detection accuracy for some attempts. To harden the IDS even further, two defense mechanisms were derived from both data-based and model-based methods. Specifically, these methods relied on feature squeezing as well as adversarial training defense strategies. They yielded promising results, enhanced robustness, and maintained standard accuracy in the presence or absence of adversaries. The obtained results proved the efficiency of the proposed framework in robustifying IDS performance within the IoT context. In particular, the accuracy reached 100% for black-box attack scenarios while preserving the accuracy in the absence of attacks as well.