bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Electromyography Bridge on Upper Limb Motor Functions in Stroke Participants: An Exploratory Randomized Controlled Trial
Authors: Zhao Qin , Jia Gongwei , Jia Lang , Wang Yule , Jiang Wei , Feng Yali , Jiang Hang , Yu Lehua , Yu Jing , Tan Botao
Published: July 2022

The electromyography bridge (EMGB) plays an important role in promoting the recovery of wrist joint function in stroke patients. We investigated the effects of the EMGB on promoting the recovery of upper limb function in hemiplegia. Twenty-four stroke patients with wrist dorsal extension dysfunction were recruited. Participants were randomized to undergo EMGB treatment or neuromuscular electrical stimulation (NMES). Treatments to wrist extensors were conducted for 25 min, twice a day, 5 days per week, for 1 month. Outcome measures: active range of motion (AROM) of wrist dorsal extension; Fugl-Meyer assessment for upper extremity (FMA-UE); Barthel index (BI); and muscle strength of wrist extensors. After interventions, patients in the NMES group had significantly greater improvement in the AROM of wrist dorsal extension at the 4th week and 1st month follow-up (p < 0.05). However, patients in the EMGB group had a statistically significant increase in AROM only at the follow-up assessment. No significant differences were observed in the AROM between the EMGB group and the NMES group (p > 0.05). For secondary outcomes in the EMGB group, compared to baseline measurements, FMA-UE, BI, extensor carpi radialis and extensor carpi ulnaris muscle strength were significantly different as early as the 4th week (p < 0.05). The muscle strength of the extensor digitorum communis muscle showed significant differences at the follow-up (p < 0.05). There were no statistically significant differences between patients in the two groups in any of the parameters evaluated (p > 0.05). The combination of EMGB or NMES with conventional treatment had similar effects on the improvement of the hemiplegic upper limb as assessed by wrist dorsal extension, FMA-UE, and activities of daily living. The improvement in both groups was maintained until 1 month after the intervention.
Selection of Olduvai Domains during Evolution: A Role for Primate-Specific Splicing Super-Enhancer and RNA Guanine Quadruplex in Bipartite NBPF Exons
Authors: Vořechovský Igor
Published: July 2022

Olduvai protein domains (also known as DUF1220 or NBPF) have undergone the greatest human-specific increase in the copy number of any coding region in the genome. Their repeat number was strongly associated with the evolutionary expansion of brain volumes, neuron counts and cognitive abilities, as well as with disorders of the autistic spectrum. Nevertheless, the domain function and cellular mechanisms underlying the positive selection of Olduvai DNA sequences in higher primates remain obscure. Here, I show that the inclusion of Olduvai exon doublets in mature transcripts is facilitated by a potent splicing enhancer that was created through duplication within the first exon. The enhancer is the strongest among the NBPF transcripts and further promotes the already high splicing activity of the unexpanded first exons of the two-exon domains, safeguarding the expanded Olduvai exon doublets in the mature transcriptome. The duplication also creates a predicted RNA guanine quadruplex that may regulate the access to spliceosomal components of the super-enhancer and influence the splicing of adjacent exons. Thus, positive Olduvai selection during primate evolution is likely to result from a combination of multiple targets in gene expression pathways, including RNA splicing.
Nature-Based Solutions (NBSs) to Mitigate Urban Heat Island (UHI) Effects in Canadian Cities
Authors: Hayes Alexander , Jandaghian Zahra , Lacasse Michael , Gaur Abhishek , Lu Henry , Laouadi Abdelaziz , Ge Hua , Wang Liangzhu
Published: July 2022

Canada is warming at double the rate of the global average caused in part to a fast-growing population and large land transformations, where urban surfaces contribute significantly to the urban heat island (UHI) phenomenon. The federal government released the strengthened climate plan in 2020, which emphasizes using nature-based solutions (NBSs) to combat the effects of UHI phenomenon. Here, the effects of two NBSs techniques are reviewed and analysed: increasing surface greenery/vegetation (ISG) and increasing surface reflectivity (ISR). Policymakers have the challenge of selecting appropriate NBSs to meet a wide range of objectives within the urban environment and Canadian-specific knowledge of how NBSs can perform at various scales is lacking. As such, this state-of-the-art review intends to provide a snapshot of the current understanding of the benefits and risks associated with the implantation of NBSs in urban spaces as well as a review of the current techniques used to model, and evaluate the potential effectiveness of UHI under evolving climate conditions. Thus, if NBSs are to be adopted to mitigate UHI effects and extreme summertime temperatures in Canadian municipalities, an integrated, comprehensive analysis of their contributions is needed. As such, developing methods to quantify and evaluate NBSs’ performance and tools for the effective implementation of NBSs are required.
High-Performance Construction Materials: Latest Advances and Prospects
Authors: Wang Lei , Tang Shengwen
Published: July 2022

Our civilization has used construction materials extensively, especially for infrastructure projects [...]
Visual Integration Relationship between Buildings and the Natural Environment Based on Eye Movement
Authors: Fei Teng , Liu Yiqing , Guo Jianchun
Published: July 2022

In current architectural practice projects, the external visual image presented by many buildings ignores the interpretation of the environment and the local context, as well as the emotional feeling of people in visual cognition. At present, some indicators in the field of architectural design can be analyzed quantitatively, but the evaluation criteria related to vision remain in the stage of relying on experience and feeling so that the design result cannot be controlled accurately. This article reports the study of the influence of building distribution form and the ratio of the shorter side to longer side of building blocks (the S/L ratio) on the visual integration relationship between buildings and the natural environment, based on eye-tracker experiments. Six actual completed projects were chosen for evaluation in two experiments. This study uses eye-tracking recorded data to investigate the influence of two elements (the distribution of building blocks and the S/L ratio of building blocks) on the visual integration relationship of buildings and environment. It provides a theoretical approach that helps to improve architects’ building-design practices when working in different natural environments.
Mandatory Policy, Innovations and the Renewable Energy Debate: A Case Study on Building Integrated Photovoltaics
Authors: Attoye Daniel Efurosibina , Tabet Aoul Kheira Anissa , Hassan Ahmed
Published: July 2022

Innovations in buildings help to reduce energy consumption and promote environmental protection and as well as the use of renewable energy technology. However, there is a conflict when the need for an innovation clashes with the financial burden and the complex adoption processes. As a result, the negative impacts of buildings remain, and the low adoption of strategic innovations remains unaddressed. This study aims to explore this challenge, the various sides of this debate and provide a practical guide which promotes energy and building-related innovations driven by policy. This paper is an extract from a recent doctoral study conducted using an exploratory qualitative model and interviews with eighty-six residents in the United Arab Emirates (UAE). Building Integrated Photovoltaics (BIPV) was selected as a case study energy innovation and the thematic analysis of the data collected suggests that BIPV adoption is limited by multiple barriers. The debate arising from the findings highlights two opposing viewpoints. One view claims that mandatory policies are necessary to promote innovation adoption. The other view argues that the merits of mandatory policy are lost since multiple barriers significantly discourage adoption in the first place. The study takes a proactive step towards resolving the debate using a systematic approach that recommends specific drivers backed by supporting policies to guide human-centered, stakeholder-driven renewable energy transition.
Obstacles to Glioblastoma Treatment Two Decades after Temozolomide
Authors: Cruz João Victor Roza , Batista Carolina , Afonso Bernardo de Holanda , Alexandre-Moreira Magna Suzana , Dubois Luiz Gustavo , Pontes Bruno , Moura Neto Vivaldo , Mendes Fabio de Almeida
Published: July 2022

Glioblastomas are considered the most common and aggressive primary brain tumor in adults, with an average of 15 months’ survival rate. The treatment is surgery resection, followed by chemotherapy with temozolomide, and/or radiotherapy. Glioblastoma must have wild-type IDH gene and some characteristics, such as TERT promoter mutation, EGFR gene amplification, microvascular proliferation, among others. Glioblastomas have great heterogeneity at cellular and molecular levels, presenting distinct phenotypes and diversified molecular signatures in each tumor mass, making it difficult to define a specific therapeutic target. It is believed that the main responsibility for the emerge of these distinct patterns lies in subcellular populations of tumor stem cells, capable of tumor initiation and asymmetric division. Studies are now focused on understanding molecular mechanisms of chemoresistance, the tumor microenvironment, due to hypoxic and necrotic areas, cytoskeleton and extracellular matrix remodeling, and in controlling blood brain barrier permeabilization to improve drug delivery. Another promising therapeutic approach is the use of oncolytic viruses that are able to destroy specifically glioblastoma cells, preserving the neural tissue around the tumor. In this review, we summarize the main biological characteristics of glioblastoma and the cutting-edge therapeutic targets that are currently under study for promising new clinical trials.
Prognostic Models Incorporating RAS Mutation to Predict Survival in Patients with Colorectal Liver Metastases: A Narrative Review
Authors: Wong Geoffrey Yuet Mun , Diakos Connie , Molloy Mark P. , Hugh Thomas J.
Published: July 2022

Recurrence and survival vary widely among patients who undergo curative-intent resection of colorectal liver metastases (CRLM). Prognostic models provide estimated probabilities of these outcomes and allow the effects of multiple potentially interacting variables to be adjusted and assessed simultaneously. Although many prognostic models based on clinicopathologic factors have been developed since the 1990s to predict survival after resection of CRLM, these models vary in their predictive performance when applied to contemporary cohorts. Rat sarcoma viral oncogene homolog (RAS) mutation status is routinely tested in patients with metastatic colorectal cancer to predict response to anti-epidermal growth factor therapy. In addition, mutations in RAS predict survival and recurrence in patients undergoing hepatectomy for CRLM. Several recent prognostic models have incorporated RAS mutation status as a surrogate of tumor biology and combined revised clinicopathologic variables to improve the prediction of recurrence and survival. This narrative review aims to evaluate the differences between contemporary prognostic models incorporating RAS mutation status and their clinical applicability in patients considered for curative-intent resection of CRLM.
Durvalumab after Sequential High Dose Chemoradiotherapy versus Standard of Care (SoC) for Stage III NSCLC: A Bi-Centric Retrospective Comparison Focusing on Pulmonary Toxicity
Authors: Wass Romana , Hochmair Maximilian , Kaiser Bernhard , Grambozov Brane , Feurstein Petra , Weiß Gertraud , Moosbrugger Raphaela , Sedlmayer Felix , Lamprecht Bernd , Studnicka Michael , Zehentmayr Franz
Published: July 2022

Introduction: The standard of care (SoC) for unresectable stage III non-small-cell lung cancer (NSCLC) is durvalumab maintenance therapy after concurrent chemoradiation in patients with PD-L1 > 1%. However, the concurrent approach is only amenable for about one-third of patients due to co-morbidities. Although sequential regimens are usually not regarded as curative, these schedules applied in a dose-escalated manner may be similarly radical as SoC. As combining high-dose radiation and durvalumab remains a question of debate this retrospective bi-center study aims to evaluate pulmonary toxicity after high-dose chemoradiotherapy beyond 70 Gy compared to SoC. Patients and Methods: Patients with NSCLC stage III received durvalumab after either sequential high-dose chemoradiation or concomitant SoC. Chemotherapy consisted of platinum combined with either pemetrexed, taxotere, vinorelbine, or gemcitabine. The primary endpoint was short-term pulmonary toxicity occurring within six months after the end of radiotherapy (RT). Results: A total of 78 patients were eligible for this analysis. 18F-FDG-PET-CT, cranial MRT, and histological/cytological verification were mandatory in the diagnostic work-up. The high-dose and SoC group included 42/78 (53.8%) and 36/78 (46.2%) patients, respectively, which were matched according to baseline clinical variables. While the interval between the end of RT and the start of durvalumab was equal in both groups (p = 0.841), more courses were administered in the high-dose cohort (p = 0.031). Pulmonary toxicity was similar in both groups (p = 0.599), whereas intrathoracic disease control was better in the high-dose group (local control p = 0.081, regional control p = 0.184). Conclusion: The data of this hypothesis-generating study suggest that sequential high-dose chemoradiation followed by durvalumab might be similar to SoC in terms of pulmonary toxicity and potentially more effective with respect to intra-thoracic disease control. Larger trials with a prospective design are warranted to validate these results.
A Multimodal Biomarker Predicts Dissemination of Bronchial Carcinoid
Authors: Reuling Ellen M. B. P. , Naves Dwayne D. , Kortman Pim C. , Broeckaert Mark A. M. , Plaisier Peter W. , Dickhoff Chris , Daniels Johannes M. A. , Radonic Teodora
Published: July 2022

Background: Curatively treated bronchial carcinoid tumors have a relatively low metastatic potential. Gradation into typical (TC) and atypical carcinoid (AC) is limited in terms of prognostic value, resulting in yearly follow-up of all patients. We examined the additional prognostic value of novel immunohistochemical (IHC) markers to current gradation of carcinoids. Methods: A retrospective single-institution cohort study was performed on 171 patients with pathologically diagnosed bronchial carcinoid (median follow-up: 66 months). The risk of developing distant metastases based on histopathological characteristics (Ki-67, p16, Rb, OTP, CD44, and tumor diameter) was evaluated using multivariate regression analysis and the Kaplan–Meier method. Results: Of 171 patients, seven (4%) had disseminated disease at presentation, and 164 (96%) received curative-intent treatment with either endobronchial treatment (EBT) (n = 61, 36%) or surgery (n = 103, 60%). Among the 164 patients, 13 developed metastases at follow-up of 81 months (IQR 45–162). Univariate analysis showed that Ki-67, mitotic index, OTP, CD44, and tumor diameter were associated with development of distant metastases. Multivariate analysis showed that mitotic count, Ki-67, and OTP were independent risk factors for development of distant metastases. Using a 5% cutoff for Ki-67, Kaplan–Meier analysis showed that the risk of distant metastasis development was significantly associated with the number of risk predictors (AC, Ki-67 ≥ 5%, and loss of OTP or CD44) (p < 0.0001). Six out of seven patients (86%) with all three positive risk factors developed distant metastasis. Conclusions: Mitotic count, proliferation index, and OTP IHC were independent predictors of dissemination at follow-up. In addition to the widely used carcinoid classification, a comprehensive analysis of histopathological variables including Ki-67, OTP, and CD44 could assist in the determination of distant metastasis risks of bronchial carcinoids.
Metabolism-Related Gene Expression in Circulating Tumor Cells from Patients with Early Stage Non-Small Cell Lung Cancer
Authors: Zafeiriadou A. , Kollias I. , Londra T. , Tsaroucha E. , Georgoulias V. , Kotsakis A. , Lianidou E. , Markou A.
Published: July 2022

Purpose: Metabolic reprogramming is now characterized as one of the core hallmarks of cancer, and it has already been shown that the altered genomic profile of metabolically rewired cancer cells can give valuable information. In this study, we quantified three Metabolism-Related Gene (MRG) transcripts in the circulating tumor cells (CTCs) of early stage NSCLC patients and evaluated their associations with epithelial and EMT markers. Experimental Design: We first developed and analytically validated highly sensitive RT-qPCR assays for the quantification of HK2, MCT1 and PHGDH transcripts, and further studied the expression of MRGs in CTCs that were isolated using a size-dependent microfluidic device (Parsortix, Angle) from the peripheral blood of: (a) 46 NSCLC patients at baseline, (b) 39/46 of these patients one month after surgery, (c) 10/46 patients at relapse and (d) 10 pairs of cancerous and adjacent non-cancerous FFPE tissues from the same NSCLC patients. Epithelial and EMT markers were also evaluated. Results: MCT1 and HK2 were differentially expressed between HD and NSCLC patients. An overexpression of MCT1 was detected in 15/46 (32.6%) and 3/10 (30%) patients at baseline and at progression disease (PD), respectively, whereas an overexpression of HK2 was detected in 30.4% and 0% of CTCs in the same group of samples. The expression levels of all tested MRGs decreased in CTCs one month after surgery, but a significant increase was noticed at the time of relapse for PHGDH and MCT1 only. The expression levels of HK2 and MCT1 were associated with the overexpression of mesenchymal markers (TWIST-1 and VIM). Conclusion: An overexpression of MRGs was observed at a high frequency in the CTCs isolated from early NSCLC patients, thereby supporting the role of MRGs in metastatic processes. The glycolytic and mesenchymal subpopulation of CTCs was significantly predominant compared to CTCs that were glycolytic but not mesenchymal-like. Our data indicate that MRGs merit further evaluation through large and well-defined cohort studies.
Photocatalytic Conversion of Fructose to Lactic Acid by BiOBr/Zn@SnO2 Material
Authors: Qin Hong-Juan , Zhang Yu-Hang , Wang Zhen , Yang Gui-Hua
Published: July 2022

Photocatalysis provides a prospective approach for achieving high-value products under mild conditions. To realize this, constructing a selective, low-cost and environmentally friendly photocatalyst is the most critical factor. In this study, BiOBr/Zn@SnO2 is fabricated by a one-pot hydrothermal synthesis method and BiOBr: SnO2 ratio is 3:1; this material is applied as photocatalyst in fructose selective conversion to lactic acid. The bandgap structure can be regulated via two-step modification, which includes Zn doping SnO2 and Zn@SnO2 coupling BiOBr. The photocatalyst shows excellent conversion efficiency in fructose and high selectivity in lactic acid generation under alkaline conditions. The conversion rate is almost 100%, and the lactic acid yield is 79.6% under optimal reaction conditions. The catalyst is highly sustainable in reusability; the lactic acid yield can reach 67.4% after five runs. The possible reaction mechanism is also proposed to disclose the photocatalysis processes.
Feature Papers to Celebrate “Environmental Catalysis”—Trends & Outlook
Authors: Lamonier Jean-François , Bogaerts Annemie
Published: July 2022

This Special Issue collects three reviews, eight articles, and two communications related to the design of catalysts for environmental applications, such as the transformation of several pollutants into harmless or valuable products [...]
High-Performance Hydrogel Based on Modified Chitosan for Removal of Heavy Metal Ions in Borehole: A Case Study from the Bahariya Oasis, Egypt
Authors: Hamza Mohammed F. , Alotaibi Saad H. , Wei Yuezhou , Mashaal Noha M.
Published: July 2022

Globally, there is a rising demand for water purification. This demand is driven by numerous factors, including economic growth, increasing population, water shortage, and deterioration of water quality. The current work highlights the manufacturing of environmentally friendly and highly efficient sorbent based on chitosan nanoparticles after successive crosslinking (using glutaraldehyde) and modification through grafting of 4-aminoazobenzene-3,4′-disulfonic acid (AZDS) as a source of sulfonic groups. First, the produced sorbent was thoroughly specified using FTIR, TGA, SEM, SEM-EDX, pHpzc, BET (nitrogen sorption desorption isotherms), and elemental analyses (EA). The sorbent was tested for the sorption of Fe(III) before application to highly contaminated iron water well samples. Next, the sorption was improved as the sulfonation process was conducted under the selected experimental conditions within 25 and 20 min with a maximum capacity of 2.7 and 3.0 mmol Fe g−1 in visible light and under UV, respectively. Then, the uptake kinetics for both techniques were fitted by the pseudo-first-order rate equation (PFORE), in which the effect of the resistance to intraparticle diffusion has remained an unneglected factor, while the Langmuir equation has fitted the sorption isotherms. After that, the efficient desorption was achieved by using 0.2 M hydrochloric acid solution, and the desorption process was as fast as the sorption process; 15 min was sufficient for complete desorption. The sorbent shows high selectivity for heavy metal ions compared to the representative elements. Finally, the sorbent was used for the removal of heavy metal ions from a highly contaminated water well in the Bahariya Oasis and appeared to be highly efficient for heavy metal removal even in a diluted solution. Accordingly, it can be implemented in the task of water treatment.
Evaluating CO2 Desorption Activity of Tri-Solvent MEA + EAE + AMP with Various Commercial Solid Acid Catalysts
Authors: Zhang Binbin , Peng Jiacheng , Li Ye , Shi Huancong , Jin Jing , Hu Jiawei , Lu Shijian
Published: July 2022

The Paris Agreement and one of its goals, “carbon neutrality,” require intensive studies on CO2 absorption and desorption processes. When searching for ways of reducing the huge energy cost of CO2 desorption in the amine scrubbing process, the combination of blended amine with solid acid catalysts turned out to be a powerful solution in need of further investigation. In this study, the tri-solvent MEA (monoethanolamine) + EAE(2-(ethylamino)ethanol) + AMP(2-amino-2-methyl-1-propanol) was prepared at: 0.2 + 2 + 2, 0.5 + 2 + 2, 0.3 + 1.5 + 2.5 and 0.2 + 1 + 3 mol/L. The heterogeneous catalytic CO2 desorptions were tested with five commercial catalysts: blended γ-Al2O3/H-ZSM-5, H-beta, H-mordenite, HND-8 and HND-580. Desorption experiments were conducted via a recirculation process with direct heating at 363 K or using temperature programming method having a range of 303–363 K. Then, the average CO2 desorption rate, heat duty and desorption factors were studied. After comparison, the order of CO2 desorption performance was found to be HND-8 > HND-580 > H-mordenite > Hβ > blended γ-Al2O3/H-ZSM-5 > no catalyst. Among the other combinations, the 0.2 + 1 + 3 mol/L MEA + EAE + AMP with HND-8 had a minimized heat duty (HD) of 589.3 kJ/mol CO2 and the biggest desorption factor (DF) of 0.0277 × (10−3 mol CO2)3/L2 kJ min. This study provided a kind of tri-solvent with catalysts as an energy-efficient solution for CO2 absorption and desorption in industrial CO2 capture pilot plants.
Recent Advances in Multicomponent Reactions Catalysed under Operationally Heterogeneous Conditions
Authors: Bosica Giovanna , Abdilla Roderick
Published: July 2022

Multicomponent reactions (MCRs) have been gaining significance and attention over the past decade because of their ability to furnish complex products by using readily available and simple starting materials while simultaneously eliminating the need to separate and purify any intermediates. More so, most of these products have been found to exhibit diverse biological activities. Another paradigm shift which has occurred contemporarily is the switch to heterogeneous catalysis, which results in additional benefits such as the reduction of waste and an increase in the safety of the process. More importantly, it allows the user to recover and reuse the catalyst for multiple runs. In summary, both methodologies adhere to the principles of green chemistry, a philosophy which needs to become overarchingly enshrined. The plethora of reactions and catalysts which have been developed gives hope that chemists are slowly changing their ideology. As a result, this review attempts to discuss multicomponent reactions catalysed by operationally heterogeneous catalysts in the past 10 years. In this review, a further distinction is made between the MCRs which lead to the formation of heterocycles and those which do not.
Indoor Air Photocatalytic Decontamination by UV–Vis Activated CuS/SnO2/WO3 Heterostructure
Authors: Enesca Alexandru , Sisman Viorel
Published: July 2022

A titania-free heterostructure based on CuS/SnO2/WO3 was obtained by a three-step sol–gel method followed by spray deposition on the glass substrate. The samples exhibit crystalline structures and homogenous composition. The WO3 single-component sample morphology consists of fibers that serve as the substrate for SnO2 development. The CuS/SnO2/WO3 heterostructure is characterized by a dense granular morphology. Photocatalytic activity was evaluated under UV–Vis radiation and indicates that the WO3 single-component sample is able to remove 41.1% of acetaldehyde (64.9 ppm) and 52.5% of formaldehyde (81.4 ppm). However, the CuS/SnO2/WO3 exhibits a superior photocatalytic activity due to a larger light spectrum absorption and lower charge carrier recombination rate, allowing the removal of 69.2% of acetaldehyde and 78.5% of formaldehyde. The reusability tests indicate that the samples have a stable photocatalytic activity after three cycle (12 h/cycle) assessments. During light irradiation, the heterostructure acted as a Z-scheme mechanism using the redox ability of the CuS conduction band electrons and the SnO2/WO3 valence band holes to generate the oxidative species required for VOC removal.
Activity and Stability of Pd Bimetallic Catalysts for Catalytic Nitrate Reduction
Authors: Sanchis Ines , Rodriguez Juan Jose , Mohedano Angel F. , Diaz Elena
Published: July 2022

In this work, we study the effect of modifying the metal loading (0.5–1.5 wt.% Pd and 0.1–1 wt.% Sn or In), the impregnation order of noble or promoter metal (Pd–Sn or Sn–Pd), and the type of promoter metal (Sn or In) during the preparation process for a Pd bimetallic catalyst, supported on γ-alumina, used in the catalytic reduction of nitrate. The deposition of the noble metal over the promoter metal, especially with Pd:Sn ratios (wt.) of 1:10 and 1:2, favored the hydrogen spillover rate and increased the H concentration on the catalyst surface, enhancing NH4+ production. On the other hand, Pd–In catalysts showed higher activity than the Sn catalysts, as well as higher NH4+ selectivity. The stability of the Pd–Sn/Al2O3 (1.5–1 wt.%) catalyst was evaluated in long-term experiments for the treatment of synthetic water (100 mg L−1 NO3−) and three different commercial drinking waters. This Pd–Sn/Al2O3 catalyst achieved a stable nitrate conversion for a duration of 50 h in the synthetic water treatment. However, the catalyst showed a significant activity loss in the presence of other ions (different to NO3−) in the reaction medium, increasing slightly the selectivity to NH4+.
Inflammasome Activation in Retinal Pigment Epithelium from Human Donors with Age-Related Macular Degeneration
Authors: Ebeling Mara C. , Fisher Cody R. , Kapphahn Rebecca J. , Stahl Madilyn R. , Shen Shichen , Qu Jun , Montezuma Sandra R. , Ferrington Deborah A.
Published: July 2022

Age-related macular degeneration (AMD), the leading cause of blindness in the elderly, is characterized by the death of retinal pigment epithelium (RPE) and photoreceptors. One of the risk factors associated with developing AMD is the single nucleotide polymorphism (SNP) found within the gene encoding complement factor H (CFH). Part of the innate immune system, CFH inhibits alternative complement pathway activation. Multi-protein complexes called inflammasomes also play a role in the innate immune response. Previous studies reported that inflammasome activation may contribute to AMD pathology. In this study, we used primary human adult RPE cell cultures from multiple donors, with and without AMD, that were genotyped for the Y402H CFH risk allele. We found complement and inflammasome-related genes and proteins at basal levels in RPE tissue and cell cultures. Additionally, treatment with rotenone, bafilomycin A, and ATP led to inflammasome activation. Overall, the response to priming and activation was similar, irrespective of disease state or CFH genotype. While these data show that the inflammasome is present and active in RPE, our results suggest that inflammasome activation may not contribute to early AMD pathology.
Integrin β3 Promotes Resistance to EGFR-TKI in Non-Small-Cell Lung Cancer by Upregulating AXL through the YAP Pathway
Authors: Sun Qi , Lu Zhihua , Zhang Yanpeng , Xue Dong , Xia Huayu , She Junjun , Li Fanni
Published: July 2022

Integrin β3 plays a key role in the resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI), but the development of integrin β3 inhibitors has been stalled due to the failure of phase III clinical trials for cancer treatment. Therefore, it is imperative to find a potentially effective solution to the problem of acquired resistance to EGFR-TKI for patients with integrin-β3 positive non-small-cell lung cancer (NSCLC) by exploring novel downstream targets and action mechanisms of integrin β3. In the present study, we observed that the expression of integrin β3 and AXL was significantly upregulated in erlotinib-resistant NSCLC cell lines, which was further confirmed clinically in tumor specimens from patients with NSCLC who developed acquired resistance to erlotinib. Through ectopic expression or knockdown, we found that AXL expression was positively regulated by integrin β3. In addition, integrin β3 promoted erlotinib resistance in NSCLC cells by upregulating AXL expression. Furthermore, the YAP pathway, rather than pathways associated with ERK or AKT, was involved in the regulation of AXL by integrin β3. To investigate the clinical significance of this finding, the current well-known AXL inhibitor R428 was tested, demonstrating that R428 significantly inhibited resistance to erlotinib, colony formation, epithelial–mesenchymal transformation and cell migration induced by integrin β3. In conclusion, integrin β3 could promote resistance to EGFR-TKI in NSCLC by upregulating the expression of AXL through the YAP pathway. Patients with advanced NSCLC, who are positive for integrin β3, might benefit from a combination of AXL inhibitors and EGFR-TKI therapy.
Endothelial Autophagy in Coronary Microvascular Dysfunction and Cardiovascular Disease
Authors: Zhao Fujie , Satyanarayana Ganesh , Zhang Zheng , Zhao Jianli , Ma Xin-Liang , Wang Yajing
Published: July 2022

Coronary microvascular dysfunction (CMD) refers to a subset of structural and/or functional disorders of coronary microcirculation that lead to impaired coronary blood flow and eventually myocardial ischemia. Amid the growing knowledge of the pathophysiological mechanisms and the development of advanced tools for assessment, CMD has emerged as a prevalent cause of a broad spectrum of cardiovascular diseases (CVDs), including obstructive and nonobstructive coronary artery disease, diabetic cardiomyopathy, and heart failure with preserved ejection fraction. Of note, the endothelium exerts vital functions in regulating coronary microvascular and cardiac function. Importantly, insufficient or uncontrolled activation of endothelial autophagy facilitates the pathogenesis of CMD in diverse CVDs. Here, we review the progress in understanding the pathophysiological mechanisms of autophagy in coronary endothelial cells and discuss their potential role in CMD and CVDs.
The Function of Autophagy as a Regulator of Melanin Homeostasis
Authors: Lee Ki Won , Kim Minju , Lee Si Hyeon , Kim Kwang Dong
Published: July 2022

Melanosomes are melanocyte-specific organelles that protect cells from ultraviolet (UV)-induced deoxyribonucleic acid damage through the production and accumulation of melanin and are transferred from melanocytes to keratinocytes. The relatively well-known process by which melanin is synthesized from melanocytes is known as melanogenesis. The relationship between melanogenesis and autophagy is attracting the attention of researchers because proteins associated with autophagy, such as WD repeat domain phosphoinositide-interacting protein 1, microtubule-associated protein 1 light chain 3, autophagy-related (ATG)7, ATG4, beclin-1, and UV-radiation resistance-associated gene, contribute to the melanogenesis signaling pathway. Additionally, there are reports that some compounds used as whitening cosmetics materials induce skin depigmentation through autophagy. Thus, the possibility that autophagy is involved in the removal of melanin has been suggested. To date, however, there is a lack of data on melanosome autophagy and its underlying mechanism. This review highlights the importance of autophagy in melanin homeostasis by providing an overview of melanogenesis, autophagy, the autophagy machinery involved in melanogenesis, and natural compounds that induce autophagy-mediated depigmentation.
Microglia Phenotypes in Aging and Neurodegenerative Diseases
Authors: Wendimu Menbere Y. , Hooks Shelley B.
Published: July 2022

Neuroinflammation is a hallmark of many neurodegenerative diseases (NDs) and plays a fundamental role in mediating the onset and progression of disease. Microglia, which function as first-line immune guardians of the central nervous system (CNS), are the central drivers of neuroinflammation. Numerous human postmortem studies and in vivo imaging analyses have shown chronically activated microglia in patients with various acute and chronic neuropathological diseases. While microglial activation is a common feature of many NDs, the exact role of microglia in various pathological states is complex and often contradictory. However, there is a consensus that microglia play a biphasic role in pathological conditions, with detrimental and protective phenotypes, and the overall response of microglia and the activation of different phenotypes depends on the nature and duration of the inflammatory insult, as well as the stage of disease development. This review provides a comprehensive overview of current research on the various microglia phenotypes and inflammatory responses in health, aging, and NDs, with a special emphasis on the heterogeneous phenotypic response of microglia in acute and chronic diseases such as hemorrhagic stroke (HS), Alzheimer’s disease (AD), and Parkinson’s disease (PD). The primary focus is translational research in preclinical animal models and bulk/single-cell transcriptome studies in human postmortem samples. Additionally, this review covers key microglial receptors and signaling pathways that are potential therapeutic targets to regulate microglial inflammatory responses during aging and in NDs. Additionally, age-, sex-, and species-specific microglial differences will be briefly reviewed.
Regeneration or Repair? The Role of Alveolar Epithelial Cells in the Pathogenesis of Idiopathic Pulmonary Fibrosis (IPF)
Authors: Confalonieri Paola , Volpe Maria Concetta , Jacob Justin , Maiocchi Serena , Salton Francesco , Ruaro Barbara , Confalonieri Marco , Braga Luca
Published: July 2022

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive interstitial lung disease (ILD) with unknown etiology in which gradual fibrotic scarring of the lungs leads to usual interstitial pneumonia (UIP) and, ultimately, to death. IPF affects three million people worldwide, and the only currently available treatments include the antifibrotic drugs nintedanib and pirfenidone, which effectively reduce fibrosis progression are, unfortunately, not effective in curing the disease. In recent years, the paradigm of IPF pathogenesis has shifted from a fibroblast-driven disease to an epithelium-driven disease, wherein, upon recurrent microinjuries, dysfunctional alveolar type II epithelial cells (ATII) are not only unable to sustain physiological lung regeneration but also promote aberrant epithelial–mesenchymal crosstalk. This creates a drift towards fibrosis rather than regeneration. In the context of this review article, we discuss the most relevant mechanisms involved in IPF pathogenesis with a specific focus on the role of dysfunctional ATII cells in promoting disease progression. In particular, we summarize the main causes of ATII cell dysfunction, such as aging, environmental factors, and genetic determinants. Next, we describe the known mechanisms of physiological lung regeneration by drawing a parallel between embryonic lung development and the known pathways involved in ATII-driven alveolar re-epithelization after injury. Finally, we review the most relevant interventional clinical trials performed in the last 20 years with the aim of underlining the urgency of developing new therapies against IPF that are not only aimed at reducing disease progression by hampering ECM deposition but also boost the physiological processes of ATII-driven alveolar regeneration.
Enhancing Hopeful Resilience Regarding Depression and Anxiety with a Narrative Method of Ordering Memory Effective in Researchers Experiencing Burnout
Authors: Nash Carol
Published: July 2022

Depression and anxiety are prevalent, persistent, and difficult to treat industrialized world mental health problems that negatively modify an individual’s life perspective through brain function imbalances—notably, in the amygdala and hippocampus. Primarily treated with pharmaceuticals and psychotherapy, the number of individuals affected plus the intensity of their suffering continues to rise post-COVID-19. Decreasing depression and anxiety is a major societal objective. An approach is investigated that considers depression and anxiety consequences of the particular method people adopt in ordering their memories. It focuses on narrative development and the acceptance of different perspectives as uniquely necessary in creating safe protection from research burnout. The method encourages thoughtful reconsideration by participants of the negative assessments of their circumstances that can lead to depression and anxiety. The aim is to determine if the method of ordering developed is helpful in reducing burnout. This is considered through inspecting and comparing group members’ feedback form results, both pre- and post-COVID-19 restrictions. The method found useful to participants in reducing research burnout through developing hopeful resilience is comparable to authentic leadership. The conclusions offered encourage psychological and neurological research with respect to this method of promoting hopeful resilience for burnout to diminish depression and anxiety.
Luminescent Sensors Based on the Assembly of Coinage Metal Nanoclusters
Authors: Ren Chenyu , Shu Tong , Du Xin , Yang Linzhi , Su Lei , Zhang Xueji
Published: July 2022

Coinage metals, such as Cu, Ag and Au, can form nanoclusters, which, when functionalized with ligands, have unique electronic and optical properties and are widely used in biomedical imaging, remote sensing, labeling, catalytic, etc. The mechanisms, structures and properties of nanocluster assemblies have been well reviewed. However, the collections and analyses of nanocluster assemblies for sensor application are few. This review examines different nanocluster sensor platforms with a focus on the assembly and analysis of the assembly processes and examples of applications.
Aptamer-Based Sensors for Thrombin Detection Application
Authors: Sun Hongzhi , Wang Nannan , Zhang Lin , Meng Hongmin , Li Zhaohui
Published: July 2022

Thrombin facilitates the aggregation of platelet in hemostatic processes and participates in the regulation of cell signaling. Therefore, the development of thrombin sensors is conducive to comprehending the role of thrombin in the course of a disease. Biosensors based on aptamers screened by SELEX have exhibited superiority for thrombin detection. In this review, we summarized the aptamer-based sensors for thrombin detection which rely on the specific recognitions between thrombin and aptamer. Meanwhile, the unique advantages of different sensors including optical and electrochemical sensors were also highlighted. Especially, these sensors based on electrochemistry have the potential to be miniaturized, and thus have gained comprehensive attention. Furthermore, concerns about aptamer-based sensors for thrombin detection, prospects of the future and promising avenues in this field were also presented.
Should We Be Screening for Ischaemic Heart Disease Earlier in Childhood?
Authors: Bassareo Pier Paolo , O’Brien Stephen T. , Dunne Esme , Duignan Sophie , Martino Eliana , Martino Francesco , Mcmahon Colin J.
Published: July 2022

Ischaemic heart disease is the most common cause of death in males and the second in the female gender. Yet we often only focus on identification and treatment of this foremost cause of death in adulthood. The review asks the question what form of coronary disease do we encounter in childhood, what predisposing factors give rise to atherosclerosis and what strategies in childhood could we employ to detect and reduce atherosclerosis development in later life.
Evaluation of Satellite-Based Air Temperature Estimates at Eight Diverse Sites in Africa
Authors: Parsons Danny , Stern David , Ndanguza Denis , Sylla Mouhamadou Bamba
Published: July 2022

High resolution satellite and reanalysis-based air temperature estimates have huge potential to complement the sparse networks of air temperature measurements from ground stations in Africa. The recently released Climate Hazards Center Infrared Temperature with Stations (CHIRTS-daily) dataset provides daily minimum and maximum air temperature estimates on a near-global scale from 1983 to 2016. This study assesses the performance of CHIRTS-daily in comparison with measurements from eight ground stations in diverse locations across Africa from 1983 to 2016, benchmarked against the ERA5 and ERA5-Land reanalysis to understand its potential to provide localized temperature information. Compared to ERA5 and ERA5-Land, CHIRTS-daily maximum temperature has higher correlation and lower bias of daily, annual mean maximum and annual extreme maximum temperature. It also exhibits significant trends in annual mean maximum temperature, comparable to those from the station data. CHIRTS-daily minimum temperatures generally have higher correlation, but larger bias than ERA5 and ERA5-Land. However, the results indicate that CHIRTS-daily minimum temperature biases may be largely systematic and could potentially be corrected for. Overall, CHIRTS-daily is highly promising as it outperforms ERA5 and ERA5-Land in many areas, and exhibits good results across a small, but diverse set of sites in Africa. Further studies in specific geographic areas could help support these findings.
Studies of the Effect of Seasonal Cycle on the Equatorial Quasi-Biennial Oscillation with a Chemistry-Climate Model
Authors: Shibata Kiyotaka
Published: July 2022

The effect of the seasonal cycle on the quasi-biennial oscillation (QBO) in the equatorial stratosphere was investigated using a chemistry-climate model (CCM) by fixing the seasonal cycle in CCM simulations. The CCM realistically reproduced the QBO in wind and ozone fields of a 30-month period in a climatological simulation (control run) under annually repeating sea surface temperature (SST) with a seasonal cycle. For the control run, four experimental simulations (perpetual runs) were made by fixing solar declination and SST on the 15th of January, April, July, and October, respectively, for about 20 years. In the three perpetual runs of January, July, and October, the QBO was maintained and persisted throughout the 20-year integration in spite of some small differences in period and amplitude among the three runs. On the other hand, the QBO in the perpetual April run began to weaken after about 15 years and the downward propagation of westerly wind stopped at about 20 hPa, resulting in the QBO’s ceasing. The cause of this QBO disappearance is related to the evolution of the background mean flow in the lower stratosphere, which filtered out the parameterized gravity waves propagating upwards farther.
What Quality of Care Means? Exploring Clinical Nurses’ Perceptions on the Concept of Quality Care: A Qualitative Study
Authors: Stavropoulou Areti , Rovithis Michael , Kelesi Martha , Vasilopoulos George , Sigala Evangelia , Papageorgiou Dimitrios , Moudatsou Maria , Koukouli Sofia
Published: July 2022

Quality is a multidimensional issue involving various features that depend on service performance and personal assessment. Clarifying the concept of quality is essential in order to further facilitate the understanding and improvement of quality in healthcare. The purpose of this study was to investigate how clinical nurses, providing care to adult medical patients, perceive and define the concept of quality nursing care. A descriptive qualitative research design was applied. A purposive sampling strategy was used to recruit nurses from the clinical sector of a general public hospital in Athens, Greece. Ten female nurses from the medical sector participated the study. Data collection was conducted through in-depth, semi-structured interviews. Conventional content analysis was used to analyze the verbatim data. Four categories were revealed from the data analysis, namely: (a) “Quality care is holistic care”, (b) “Good care is an interpersonal issue”, (c) “Leadership is crucial”, and (d) “Best care is our responsibility”. Quality care was defined as holistic care, addressing all patient needs with competency and aiming for the best patient outcomes. It was associated with communication, teamwork, good leadership, and personal commitment. By developing an in-depth and mutual understanding about what quality means, nurse leaders and practitioners may collaborate in finding common paths to support quality interventions and enhance quality nursing care in clinical practice.