bluuebunny/crossref_metadata_2025 huggingface dataset:
Computer-Aided Prediction, Synthesis, and Characterization of Magnetic Molecularly Imprinted Polymers for the Extraction and Determination of Tolfenpyrad in Lettuce
Authors: Chi Du , Wang Wei , Mu Shiyin , Chen Shilin , Zhang Kankan
Published: March 2023

Tolfenpyrad, a pyrazolamide insecticide, can be effectively used against pests resistant to carbamate and organophosphate insecticides. In this study, a molecular imprinted polymer using tolfenpyrad as a template molecule was synthesized. The type of functional monomer and the ratio of functional monomer to template were predicted by density function theory. Magnetic molecularly imprinted polymers (MMIPs) were synthesized using 2-vinylpyridine as a functional monomer in the presence of ethylene magnetite nanoparticles at a monomer/tolfenpyrad ratio of 7:1. The successful synthesis of MMIPs is confirmed by the results of the characterization analysis by scanning electron microscopy, nitrogen adsorption–desorption isotherms, Fourier transform infrared spectroscopy, X-ray diffractometer, thermogravimetric analyzer, and vibrational sample magnetometers. A pseudo-second-order kinetic model fit the adsorption of tolfenpyrad, and the kinetic data are in good agreement with the Freundlich isothermal model. The adsorption capacity of the polymer to the target analyte was 7.20 mg/g, indicating an excellent selective extraction capability. In addition, the adsorption capacity of the MMIPs is not significantly lost after several reuses. The MMIPs showed great analytical performance in tolfenpyrad-spiked lettuce samples, with acceptable accuracy (intra- and inter-day recoveries of 90.5–98.8%) and precision (intra- and inter-day relative standard deviations of 1.4–5.2%).
Physicochemical and Functional Changes in Lotus Root Polysaccharide Associated with Noncovalent Binding of Polyphenols
Authors: Liu Qiulan , Zou Xiaoqin , Yi Yang , Sun Ying , Wang Hongxun , Jiang Xueyu , Peng Kaidi
Published: March 2023

To promote the functional applications of lotus root polysaccharides (LRPs), the effects of noncovalent polyphenol binding on their physicochemical properties, as well as antioxidant and immunomodulatory activities, were investigated. Ferulic acid (FA) and chlorogenic acid (CHA) were spontaneously bound to the LRP to prepare the complexes LRP-FA1, LRP-FA2, LRP-FA3, LRP-CHA1, LRP-CHA2 and LRP-CHA3, and their mass ratios of polyphenol to LRP were, respectively, 121.57, 61.18, 34.79, 2359.58, 1276.71 and 545.08 mg/g. Using the physical mixture of the LRP and polyphenols as a control, the noncovalent interaction between them in the complexes was confirmed by ultraviolet and Fourier-transform infrared spectroscopy. The interaction increased their average molecular weights by 1.11~2.27 times compared to the LRP. The polyphenols enhanced the antioxidant capacity and macrophage-stimulating activity of the LRP depending on their binding amount. Particularly, the DPPH radical scavenging activity and FRAP antioxidant ability were positively related to the FA binding amount but negatively related to the CHA binding amount. The NO production of the macrophages stimulated by the LRP was inhibited by the co-incubation with free polyphenols; however, the inhibition was eliminated by the noncovalent binding. The complexes could stimulate the NO production and tumor necrosis factor-α secretion more effectively than the LRP. The noncovalent binding of polyphenols may be an innovative strategy for the structural and functional modification of natural polysaccharides.
Influence of Biotreatment on Hordeum vulgare L. Cereal Wholemeal Contamination and Enzymatic Activities
Authors: Juodeikiene Grazina , Trakselyte-Rupsiene Karolina , Reikertaite Karolina , Janic Hajnal Elizabet , Bartkevics Vadims , Pugajeva Iveta , Gruzauskas Valentas , Švazas Mantas , Gruzauskas Romas , Santini Antonello , Rocha João Miguel , Bartkiene Elena
Published: March 2023

Crop contamination with mycotoxins is a global problem with a negative impact on human and animal health as well as causing economical losses in food and feed chains. This study was focused on the evaluation of the effect of lactic acid bacteria (LAB) strain (Levilactobacillus brevis-LUHS173, Liquorilactobacillus uvarum-LUHS245, Lactiplantibacillus plantarum-LUHS135, Lacticaseibacillus paracasei-LUHS244 and Lacticaseibacillus casei-LUHS210) fermentation on the changes in the level of deoxynivalenol (DON) and its conjugates in Fusarium spp.-contaminated barley wholemeal (BWP). Samples, with different contamination of DON and its conjugates, were treated separately (for 48 h). In addition to mycotoxin content, enzymatic activities (amylolytic, xylanolytic, and proteolytic) of BWP (before and after fermentation) were evaluated. It was established that the effect of decontamination depends on the LAB strain used, and a significant reduction in DON and the concentration of its conjugates in Lc. casei fermented samples was achieved: the amount of DON decreased on average by 47%, and the amount of D3G, 15-ADON and 3-ADON decreased by 82.4, 46.1, and 55.0%, respectively. Lc. casei also showed viability in the contaminated fermentation medium and an effective production of organic acids was obtained. Additionally, it was found that enzymes are involved to the detoxification mechanism of DON and its conjugates in BWP. These findings indicate that fermentation with selected LAB strains could be applied for contaminated barley treatment in order to significantly reduce Fusarium spp. mycotoxin levels in BWP and improve the sustainability of grain production.
Recent Advances on Main Active Ingredients, Pharmacological Activities of Rosa roxbughii and Its Development and Utilization
Authors: Wang Li , Wei Tingting , Zheng Li , Jiang Fangfang , Ma Wentao , Lu Min , Wu Xiaomao , An Huaming
Published: March 2023

Rosa roxburghii tratt (R. roxburghii) is an important plant resource that is widely distributed in the southwest of China and favored by consumers due to its high nutritional value and healthy functions. Meanwhile, it is a traditional edible and medicinal plant in China. With the deepening research of R. roxburghii, more and more bioactive components and its health care and medicinal value have been discovered and developed in recent years. This review summarizes and discusses the recent advances on main active ingredients such as vitamin, protein, amino acid, superoxide dismutase, polysaccharide, polyphenol, flavonoid, triterpenoid and mineral, and pharmacological activities including antioxidant activity, immunomodulatory activity, anti-tumor activity, glucose and lipid metabolism regulation, anti-radiation effect, detoxification effect, and viscera protection of R. roxbughii, as well as its development and utilization. The research status and existing problems of R. roxburghii development and quality control are also briefly introduced. This review ends with some suggestions on the perspectives and directions for future research and potential applications of R. roxbughii.
Polyphenol-Dietary Fiber Conjugates from Fruits and Vegetables: Nature and Biological Fate in a Food and Nutrition Perspective
Authors: Fernandes Ana , Mateus Nuno , de Freitas Victor
Published: March 2023

In the past few years, numerous studies have investigated the correlation between polyphenol intake and the prevention of several chronic diseases. Research regarding the global biological fate and bioactivity has been directed to extractable polyphenols that can be found in aqueous-organic extracts, obtained from plant-derived foods. Nevertheless, significant amounts of non-extractable polyphenols, closely associated with the plant cell wall matrix (namely with dietary fibers), are also delivered during digestion, although they are ignored in biological, nutritional, and epidemiological studies. These conjugates have gained the spotlight because they may exert their bioactivities for much longer than extractable polyphenols. Additionally, from a technological food perspective, polyphenols combined with dietary fibers have become increasingly interesting as they could be useful for the food industry to enhance technological functionalities. Non-extractable polyphenols include low molecular weight compounds such as phenolic acids and high molecular weight polymeric compounds such as proanthocyanidins and hydrolysable tannins. Studies concerning these conjugates are scarce, and usually refer to the compositional analysis of individual components rather than to the whole fraction. In this context, the knowledge and exploitation of non-extractable polyphenol-dietary fiber conjugates will be the focus of this review, aiming to access their potential nutritional and biological effect, together with their functional properties.
Compositional Traits of Grains and Groats of Barley, Oat and Spelt Grown at Organic and Conventional Fields
Authors: Sinkovič Lovro , Rakszegi Marianna , Pipan Barbara , Meglič Vladimir
Published: March 2023

Barley, oats, or spelt consumed as minimally processed whole grains provide several health benefits, especially when grown under organic field management conditions. Therefore, the effects of organic and conventional farming on the compositional traits (protein, fibre, fat, and ash) of barley, oat, and spelt grains and groats were compared using three winter barley varieties (‘Anemone’, ‘BC Favorit’, and ‘Sandra’), two spring oat varieties (‘Max’ and ‘Noni’), and three spelt varieties (‘Ebners Rotkorn’, ‘Murska bela’, and ‘Ostro’). Groats were produced from harvested grains by a combination of threshing, winnowing, and brushing/polishing. Multitrait analysis showed significant differences between species, field management practices, and fractions, with clear compositional differences between organic and conventional spelt. Barley and oat groats had a higher thousand kernel weight (TKW) and β-glucan, but lower crude fibre, fat, and ash contents than the grains. The composition of the grains of the different species differed significantly for more traits (TKW, fibre, fat, ash, and β-glucan) than that of the groats (TKW and fat), while field management only affected the fibre content of the groats and the TKW, ash, and β-glucan contents of the grains. The TKW, protein, and fat contents of the different species differed significantly under both conventional and organic growing conditions, while the TKW and fibre contents of grains and groats differed under both systems. The caloric value of the final products of barley, oats, and spelt groats ranged from 334–358 kcal/100 g. This information will be useful for not only the processing industry, but also for breeders and farmers, and last, but not least, for consumers.
Impact on Glycemic Variation Caused by a Change in the Dietary Intake Sequence
Authors: Alonso-Bastida Alexis , Adam-Medina Manuel , Salazar-Piña Dolores-Azucena , Escobar-Jiménez Ricardo-Fabricio , Parra-Cabrera María-Socorro , Cervantes-Bobadilla Marisol
Published: March 2023

This work presents an analysis of the effect on glycemic variation caused by modifying the macronutrient intake sequence in a person without a diagnosis of diabetes. In this work, three types of nutritional studies were developed: (1) glucose variation under conditions of daily intake (food mixture); (2) glucose variation under conditions of daily intake modifying the macronutrient intake sequence; (3) glucose variation after a modification in the diet and macronutrient intake sequence. The focus of this research is to obtain preliminary results on the effectiveness of a nutritional intervention based on the modification of the sequence of macronutrient intake in a healthy person during 14-day periods. The results obtained corroborate the positive effect on the glucose of consuming vegetables, fiber, or proteins before carbohydrates, decreasing the peaks in the postprandial glucose curves (vegetables: 113–117 mg/dL; proteins: 107–112 mg/dL; carbohydrates: 115–125 mg/dL) and reducing the average levels of blood glucose concentrations (vegetables: 87–95 mg/dL; proteins: 82–99 mg/dL; carbohydrates: 90–98 mg/dL). The present work demonstrates the preliminary potential of the sequence in the macronutrient intake for the generation of alternatives of prevention and solution of chronic degenerative diseases, improving the management of glucose in the organism and permeating in the reduction of weight and the state of health of the individuals.
Process Optimization, Structural Characterization, and Calcium Release Rate Evaluation of Mung Bean Peptides-Calcium Chelate
Authors: Zhai Wenliang , Lin Dong , Mo Ruoshuang , Zou Xiaozhuan , Zhang Yongqing , Zhang Liyun , Ge Yonghui
Published: March 2023

To reduce grievous ecological environment pollution and protein resource waste during mung bean starch production, mung bean peptides-calcium chelate (MBP-Ca) was synthesized as a novel and efficient calcium supplement. Under the optimal conditions (pH = 6, temperature = 45 °C, mass ratio of mung bean peptides (MBP)/CaCl2 = 4:1, MBP concentration = 20 mg/mL, time = 60 min), the obtained MBP-Ca achieved a calcium chelating rate of 86.26%. MBP-Ca, different from MBP, was a new compound rich in glutamic acid (32.74%) and aspartic acid (15.10%). Calcium ions could bind to MBP mainly through carboxyl oxygen, carbonyl oxygen, and amino nitrogen atoms to form MBP-Ca. Calcium ions-induced intra- and intermolecular interactions caused the folding and aggregation of MBP. After the chelation reaction between calcium ions and MBP, the percentage of β-sheet in the secondary structure of MBP increased by 1.90%, the size of the peptides increased by 124.42 nm, and the dense and smooth surface structure of MBP was transformed into fragmented and coarse blocks. Under different temperatures, pH, and gastrointestinal simulated digestion conditions, MBP-Ca exhibited an increased calcium release rate compared with the conventional calcium supplement CaCl2. Overall, MBP-Ca showed promise as an alternative dietary calcium supplement with good calcium absorption and bioavailability.
Incidence of Potentially Toxic Elements and Perfluoroalkyl Substances Present in Canned Anchovies and Their Impact on Food Safety
Authors: Nobile Maria , Mosconi Giacomo , Chiesa Luca Maria , Panseri Sara , Danesi Luigi , Falletta Ermelinda , Arioli Francesco
Published: March 2023

Fish plays a key role in a healthy and balanced Italian diet, but it is also subject to the bioaccumulation of different contaminants depending on the geographical or anthropogenic context from which it is derived. In recent years, the European Food Safety Authority (EFSA) has been focusing its attention on consumer toxicological risk, considering emerging contaminants such as perfluoroalkyl substances (PFASs) and potentially toxic elements (PTEs). Regarding fish, anchovies are among the five small pelagic main commercial species in the European Union and the top five fresh species consumed by households in Italy. Considering the lack of data on PFASs and PTEs in this species, our aim was to investigate the mentioned contaminants in salted and canned anchovies collected over 10 months from different fishing areas, even those far apart, to verify possible variations in bioaccumulation and to consider the risk for the consumer. According to our results, the assessed risk was very reassuring also for large consumers. The only concern, related to Ni acute toxicity, also dependent on the different consumers’ sensitivity, was related to only one sample.
Analysing the Potential of Performing Last-Mile Bicycle Deliveries: The Perspective of Brazilian Logistics Operators
Authors: Nascimento Carla de Oliveira Leite , Oliveira Leise Kelli de
Published: March 2023

Knowledge about the perception of stakeholders regarding the effectiveness of last-mile bicycle deliveries is essential to promote solutions at a local and national level. However, their use is an incipient solution in the Brazilian context. This paper aims to analyse the potential of performing last-mile bicycle deliveries according to the perspective of Brazilian logistics operators. First, the variables that affect the decision of logistic operators to adopt bicycles were captured using the theory of planned behaviour. Later, the intention of behaviour was measured by estimating an ordered logistic regression. The results showed that environmental awareness, public policies and weather conditions negatively influence the intention of logistics operators to perform last-mile bicycle deliveries in Brazil. However, these variables are more cultural than operational, and could be changed by awareness of the importance of performing last-mile bicycle deliveries to promote sustainable cities. Additionally, the findings could encourage the development of sustainable freight policies from private companies to achieve sustainable future transportation.
Determination of Foetal Scalp Blood Sampling pH as an Indicator of Loss of Foetal Well-Being in Women Undergoing Caesarean Section
Authors: Alarcón-Rodríguez Raquel , Martín-Álvarez María Paz , García-González Jessica , Requena-Mullor Mar , Rodríguez-García Mª Carmen , Zheng Ruirui , Rey Raúl Romero-del
Published: March 2023

Asphyxia during birth is one of the three leading causes of neonatal morbidity and mortality among newborns carried to term. The objective of this study was to evaluate the measurement of the foetal scalp blood pH as a measure of foetal status, evaluating: cord gases, meconium-stained fluid, APGAR score or the need for neonatal resuscitation in pregnant women undergoing caesarean sections. A cross-sectional study was carried out over a period of 5 years (2017–2021) at the Hospital de Poniente (southern Spain). A total of 127 pregnant women participated from whom a foetal scalp blood pH sample was taken and used to indicate the need for an urgent caesarean section. The results showed a correlation between the pH of the scalp blood and the pH of the umbilical cord artery, umbilical cord vein (Rho of Spearman arterial pH: 0.64, p < 0.001; Rho of Spearman venous pH: 0.58, p < 0.001) and the APGAR test one minute after delivery (Spearman’s Rho coefficient of 0.33, p < 0.01). These results suggest that the foetal scalp pH should not be considered a foolproof method to indicate an urgent caesarean section. Foetal scalp pH sampling can be used as a complementary test, in conjunction with cardiotocography, to indicate whether an emergency caesarean section is necessary due to loss of foetal well-being.
Prevalence of Malnutrition in Hospitalized Patients in Lebanon Using Nutrition Risk Screening (NRS-2002) and Global Leadership Initiative on Malnutrition (GLIM) Criteria and Its Association with Length of Stay
Authors: Ouaijan Krystel , Hwalla Nahla , Kandala Ngianga-Bakwin , Mpinga Emmanuel Kabengele
Published: March 2023

(1) Background: Prevalence studies on hospital malnutrition are still scarce in the Middle East region despite recent global recognition of clinical malnutrition as a healthcare priority. The aim of this study is to measure the prevalence of malnutrition in adult hospitalized patients in Lebanon using the newly developed Global Leadership Initiative on Malnutrition tool (GLIM), and explore the association between malnutrition and the length of hospital stay (LOS) as a clinical outcome. (2) Methods: A representative cross-sectional sample of hospitalized patients was selected from a random sample of hospitals in the five districts in Lebanon. Malnutrition was screened and assessed using the Nutrition Risk Screening tool (NRS-2002) and GLIM criteria. Mid-upper arm muscle circumference (MUAC) and handgrip strength were used to measure and assess muscle mass. Length of stay was recorded upon discharge. (3) Results: A total of 343 adult patients were enrolled in this study. The prevalence of malnutrition risk according to NRS-2002 was 31.2%, and the prevalence of malnutrition according to the GLIM criteria was 35.6%. The most frequent malnutrition-associated criteria were weight loss and low food intake. Malnourished patients had a significantly longer LOS compared to patients with adequate nutritional status (11 days versus 4 days). Handgrip strength and MUAC measurements were negatively correlated with the length of hospital stay. (4) Conclusion and recommendations: the study documented the valid and practical use of GLIM for assessing the prevalence and magnitude of malnutrition in hospitalized patients in Lebanon, and highlighted the need for evidence-based interventions to address the underlying causes of malnutrition in Lebanese hospitals.
Effectiveness of a Combined Toothbrushing Technique on Cariogenic Dental Biofilm in Relation to Stainless Steel and Elastomeric Ligatures in Orthodontic Patients: A Randomized Clinical Trial
Authors: Saengphen Thanakorn , Koontongkaew Sittichai , Utispan Kusumawadee
Published: March 2023

Increased dental biofilm commonly occurs during orthodontic treatment. The aim of this study was to evaluate the effect of a combined toothbrushing method on dental biofilm cariogenicity in patients with stainless steel (SSL) and elastomeric (EL) ligatures. At baseline (T1), 70 participants were randomized (1:1 ratio) to the SSL or EL group. Dental biofilm maturity was evaluated using a three-color-disclosing dye. The participants were instructed to brush their teeth using a combined horizontal–Charters–modified Bass technique. Dental biofilm maturity was reassessed at the 4-week follow-up (T2). We found that at T1, new dental biofilm was the highest, followed by mature and cariogenic dental biofilm in the SSL group (p < 0.05). In the EL group, cariogenic dental biofilm was highly observed, followed by mature and new dental biofilm (p < 0.05). After intervention, cariogenic dental biofilm significantly decreased in both groups (p < 0.05). Moreover, a marked decrease in cariogenic dental biofilm was observed in the EL group compared with the SSL group (p < 0.05). However, the change in mature dental biofilm in the groups was similar (p > 0.05). Our results demonstrated that the combined toothbrushing method reduced cariogenic dental biofilm in the SSL and EL groups.
Importance of Asprosin for Changes of M. Rectus Femoris Area during the Acute Phase of Medical Critical Illness: A Prospective Observational Study
Authors: Sipahioglu Hilal , Ilik Hatice Kubra Zenger , Ozer Nurhayat Tugra , Onuk Sevda , Koyuncu Sumeyra , Kuzuguden Sibel , Elay Gulseren
Published: March 2023

Asprosin, a new adipokine, is secreted by subcutaneous white adipose tissue and causes rapid glucose release. The skeletal muscle mass gradually diminishes with aging. The combination of decreased skeletal muscle mass and critical illness may cause poor clinical outcomes in critically ill older adults. To determine the relationship between the serum asprosin level, fat-free mass, and nutritional status of critically ill older adult patients, critically ill patients over the age of 65 receiving enteral nutrition via feeding tube were included in the study. The patients’ cross-sectional area of the rectus femoris (RF) of the lower extremity quadriceps muscle was evaluated by serial measurements. The mean age of the patients was 72 ± 6 years. The median (IQR) serum asprosin level was 31.8 (27.4–38.1) ng/mL on the first study day and 26.1 (23.4–32.3) ng/mL on the fourth study day. Serum asprosin level was high in 96% of the patients on the first day, and it was high in 74% on the fourth day after initiation of enteral feeding. The patients achieved 65.9 ± 34.1% of the daily energy requirement for four study days. A significant moderate correlation between delta serum asprosin level and delta RF was found (Rho = −0.369, p = 0.013). In critically ill older adult patients, a significant negative correlation was determined between serum asprosin level with energy adequacy and lean muscle mass.
Constrictive Pericarditis–A Cloak Camouflaging Lymphoma
Authors: Venugopala Delanthabettu , Dsouza Nikhil Victor , Acharya Vishak , Rai Maneesh , Venkataramana Chaithra Gowthuvalli , Boussios Stergios
Published: March 2023

Non-Hodgkin’s lymphoma presenting as a primary cardiac lymphoma (PCL) is extremely unusual. Having a predilection for the right side of the heart and accounting for 1% of all cardiac tumours, the difficulty in diagnosing the lesion, owing to the location and vague presenting symptoms and signs, often leads to delayed diagnosis and poor prognosis. In our case report, a middle-aged male was diagnosed with PCL presenting as pyrexia of unknown origin with the help of F18-fluorodeoxyglucose positron emission tomography (18 FDG-PET). PET-CT is an invaluable tool in patients with pyrexia of unknown origin (PUO), especially caused by neoplasms as it helps in localizing the target lesion, aiding in selecting the appropriate intervention for rapid tissue diagnosis. This case serves to sensitize the physicians of PCL presenting with PUO and mimicking a relatively common cardiac tumour such as atrial myxoma.
Effects of Geographical Area and Harvest Times on Chemical Composition and Antibacterial Activity of Juniperus communis L. Pseudo-Fruits Extracts: A Statistical Approach
Authors: Popescu Diana , Botoran Oana , Cristea Ramona , Mihăescu Cristina , Șuțan Nicoleta
Published: March 2023

The beneficial effects of Juniperus communis L. extracts have been known for a long time. Therefore a scientific knowledge of the chemical profile leading to this bioactivity is required. The aim of this paper is to highlight the influence of geographical origin and harvest time on compositional elements of hydroalcoholic extracts of J. communis pseudo-fruits, but also on their antibacterial properties. The samples were collected from three mountainous area of Romania, during three consecutive months. The total polyphenols were determined by the Folin-Ciocâlteu method, ranging between 12.67 and 14.77 mg GAE/g DW. High-performance liquid chromatography (HPLC) analysis was applied to identify fifteen phenolic compounds from the group of phenolic acids and flavonoids. The antibacterial effect was assessed on Gram-positive and Gram-negative bacterial strains. A good antimicrobial activity was achieved by the extracts of pseudo-fruits harvested during October and November from the Iezerele Cindrelului Botanical Nature Reserve. The obtained results denote a diverse composition of active principles in common juniper pseudo-fruits and moderate antibacterial activity dependent on the harvest time and geographical area.
Exploring Effects of Exogenous Selenium on the Growth and Nutritional Quality of Cabbage (Brassica oleracea var. capitata L.)
Authors: Yu Li , Chen Qiangwen , Liao Xiaoli , Yang Xiaoyan , Chao Wei , Cong Xin , Zhang Weiwei , Liao Yongling , Ye Jiabao , Qian Hua , Zhao Yang , Cheng Shuiyuan , Xu Feng
Published: March 2023

Selenium (Se) is an important trace element in human and animal health. Approximately 0.5–1 billion people worldwide are facing Se deficiency which can result in various endemic diseases. Cabbage is one of the most popular vegetables and can accumulate Se through biofortification. Therefore, producing Se-enriched cabbage may be an effective method to alleviate Se deficiency. In this study, the effects of different concentrations of selenite application on the growth and nutritional quality of cabbage were investigated and the results showed that the growth of cabbage was promoted at low concentrations of selenite (0.1–0.4 mmol/L) but inhibited at high concentrations (0.8–1.6 mmol/L). Concentrations of 0.1–0.2 mmol/L of selenite induced the accumulation of primary metabolites (soluble proteins, soluble sugars, and free amino acids), representative secondary metabolites (ascorbic acid, glucosinolates, flavonoids, anthocyanins, and phenolic acids), and important antioxidant enzyme (glutathione peroxidase, catalase, and superoxide dismutase) activity to improve the nutritional quality of cabbages. In addition, a higher concentration (0.8–1.6 mmol/L) of selenite proved beneficial in the accumulation of total Se and representative organic Se in cabbages. The main organic Se species in cabbage were selenomethionine (SeMet), accounting for 12.10% of total Se, followed by selenocysteine (SeCys2), accounting for 2.96% of total Se. It is suggested that an appropriate dose of exogenous selenite could be selected for different production purposes in cabbage cultivation. These findings are helpful for us to deeply understand the effects of selenite on the growth and nutritional quality of cabbages and to provide reliable technical support for vegetable cultivation and Se biofortification.
Diversity and Seasonality of Aquatic Beetles (Coleoptera) in Three Localities of the State of Tlaxcala, Central Mexico
Authors: Luna-Luna Alba Magali , Martins Caleb Califre , Lara Carlos , Contreras-Ramos Atilano
Published: March 2023

Aquatic beetle diversity was compared between three study sites in the state of Tlaxcala, central Mexico: Stream 1 (San Ambrosio), stream 2 (San Tadeo), and a lake (Acuitlapilco). Sampling took place bimonthly during an annual cycle. A total of 2968 specimens were obtained, which were grouped into twenty three species, fifteen genera, and six families (Elmidae, Dryopidae, Dytiscidae, Gyrinidae, Haliplidae, and Hydrophilidae). Stream 2 showed the highest values of abundance (1570 individuals), while stream 1 had the highest richness (18 species). Abundance values showed two peaks each in rainy and dry seasons. The proportion of rare and dominant species was similar in all study sites. Based on species accumulation curves, the maximum estimated number of species has not been achieved in either of the three sites. Regarding alpha diversity (effective number of species), stream 1 presented the highest zero-order diversity estimated with 18.5 species. Regarding beta diversity, lotic systems (streams 1 and 2) presented a similarity of 75%. Finally, regarding the trophic structure of the adult aquatic beetle community, herbivores, predators, and decomposers were most representative in this study. Based on our results, Tlaxcala probably holds a significant diversity of aquatic beetles. This appears to indicate that species composition in geographic areas, regardless of their relatively small size, is worth documenting and, of course, preserving.
Exploring Historical Australian Expeditions with Time-Layered Cultural Maps
Authors: Yu Feiyan , Champion Erik , McMeekin David
Published: March 2023

The Australian Time Layered Cultural Map platform was created to help digital humanities scholars investigate how online geospatial tools could provide exemplars to their humanities colleagues on how historical collections and cultural data could be extended and re-examined with geospatial tools. The project discussed here investigated how Recogito/TMT could effectively extract spatial and temporal data from pure text-based historical information and generate time-layered interactive maps of that spatio-temporal data using accessible and user-friendly software. The target audience was humanities scholars relatively new to geospatial technologies and relevant programming systems. The interactive maps were created with two free, open-source web applications and one commercial GIS (Geographic Information System) mapping application. The relative pros and cons of each application are discussed. This paper also investigates simple workflows for extracting spatiotemporal data into RDF (Resource Description Framework) format to be used as Linked Open Data.
LPG-Based Knowledge Graphs: A Survey, a Proposal and Current Trends
Authors: Di Pierro Davide , Ferilli Stefano , Redavid Domenico
Published: March 2023

A significant part of the current research in the field of Artificial Intelligence is devoted to knowledge bases. New techniques and methodologies are emerging every day for the storage, maintenance and reasoning over knowledge bases. Recently, the most common way of representing knowledge bases is by means of graph structures. More specifically, according to the Semantic Web perspective, many knowledge sources are in the form of a graph adopting the Resource Description Framework model. At the same time, graphs have also started to gain momentum as a model for databases. Graph DBMSs, such as Neo4j, adopt the Labeled Property Graph model. Many works tried to merge these two perspectives. In this paper, we will overview different proposals aimed at combining these two aspects, especially focusing on possibility for them to add reasoning capabilities. In doing this, we will show current trends, issues and possible solutions. In this context, we will describe our proposal and its novelties with respect to the current state of the art, highlighting its current status, potential, the methodology, and our prospect.
Metal Wire Additive Manufacturing: A Comparison between Arc Laser and Laser/Arc Heat Sources
Authors: Casalino Giuseppe , Karamimoghadam Mojtaba , Contuzzi Nicola
Published: March 2023

In this paper, the authors introduce the reader to the state of the art of Metal Wire Additive Manufacturing (MWAM) and provide a comparison between Wire Arc Additive Manufacturing (WAAM), Wire Laser Additive Manufacturing (WLAM), and Laser Arc Hybrid Wire Deposition (LAHWD) based on their characteristics and potential future applications, since MWAM is expected to have a promising future in various areas, such as aerospace, automotive, biomedical, and energy fields. A detailed discussion of the benefits and drawbacks of each Metal Wire Additive Manufacturing process can help to improve our understanding of the unique characteristics of metal wire application. Therefore, this paper offers a comprehensive analysis that can serve as a reference for upcoming industrial projects and research initiatives, with the aim of helping industries choose the most appropriate WAM technique for their specific applications.
Impact of COVID-19 on the Surrounding Environment of Nursing Home Residents and Attitudes towards Infection Control and Oral Health Care among Nursing Home Staff in Japan
Authors: Hidaka Rena , Matsuo Koichiro , Maruyama Tomoka , Kawasaki Kyoka , Tasaka Itsuki , Arai Masami , Sakoda Satoshi , Higuchi Kazunori , Jinno Erina , Yamada Tsuyoshi , Minakuchi Shunsuke
Published: March 2023

The environments of nursing home staff and residents have dramatically changed since the onset of the COVID-19 pandemic, with greater demand for infection control. This study aimed to clarify the changes and regional differences in the surrounding environment of nursing home residents as well as the working environment of staff, including oral health care, after the spread of SARS-CoV-2. A self-administered questionnaire survey was sent to nursing staff at about 40 nursing homes in different areas of Japan in September and October 2021. The questionnaire consisted of items centered around: (1) the surrounding environment of nursing home residents, (2) awareness and attitudes towards daily work among staff, and (3) attitudes to and procedures for oral health care among staff. A total of 929 respondents included 618 (66.5%) nursing care workers and 134 (14.4%) nurses. Regarding changes in resident daily life, 60% of staff perceived decreases in psychosocial and physical function after the start of the pandemic due to limited family communication and recreational activities, especially in urban areas. Concerning infection control, most respondents adopted routines of disinfecting hands before and after their duties. Oral health care was part of the regular duties of over 80% of respondents. Many participants answered that the frequency and time of oral health care only slightly changed after the onset of COVID-19, but many also reported disinfecting hands both before and after oral health care, particularly in rural areas. Our findings suggested that the COVID-19 pandemic decreased the daily living activities of residents, leading to psychosocial and physical decline, especially in urban areas. The results also indicated that the spread of SARS-CoV-2 triggered improvements in the awareness and attitudes towards infection control in daily work, including oral health care, among nursing care staff, notably in rural areas. Such an effect may contribute to a more positive perception of oral health care infection measures after the pandemic.
Gut Microbiota in Chronic Kidney Disease: From Composition to Modulation towards Better Outcomes—A Systematic Review
Authors: Voroneanu Luminita , Burlacu Alexandru , Brinza Crischentian , Covic Andreea , Balan Gheorghe G. , Nistor Ionut , Popa Cristina , Hogas Simona , Covic Adrian
Published: March 2023

Background: A bidirectional kidney–gut axis was described in patients with chronic kidney disease (CKD). On the one hand, gut dysbiosis could promote CKD progression, but on the other hand, studies reported specific gut microbiota alterations linked to CKD. Therefore, we aimed to systematically review the literature on gut microbiota composition in CKD patients, including those with advanced CKD stages and end-stage kidney disease (ESKD), possibilities to shift gut microbiota, and its impact on clinical outcomes. Materials and methods: We performed a literature search in MEDLINE, Embase, Scopus, and Cochrane databases to find eligible studies using pre-specified keywords. Additionally, key inclusion and exclusion criteria were pre-defined to guide the eligibility assessment. Results: We retrieved 69 eligible studies which met all inclusion criteria and were analyzed in the present systematic review. Microbiota diversity was decreased in CKD patients as compared to healthy individuals. Ruminococcus and Roseburia had good power to discriminate between CKD patients and healthy controls (AUC = 0.771 and AUC = 0.803, respectively). Roseburia abundance was consistently decreased in CKD patients, especially in those with ESKD (p < 0.001). A model based on 25 microbiota dissimilarities had an excellent predictive power for diabetic nephropathy (AUC = 0.972). Several microbiota patterns were observed in deceased ESKD patients as compared to the survivor group (increased Lactobacillus, Yersinia, and decreased Bacteroides and Phascolarctobacterium levels). Additionally, gut dysbiosis was associated with peritonitis and enhanced inflammatory activity. In addition, some studies documented a beneficial effect on gut flora composition attributed to synbiotic and probiotic therapies. Large randomized clinical trials are required to investigate the impact of different microbiota modulation strategies on gut microflora composition and subsequent clinical outcomes. Conclusions: Patients with CKD had an altered gut microbiome profile, even at early disease stages. Different abundance at genera and species levels could be used in clinical models to discriminate between healthy individuals and patients with CKD. ESKD patients with an increased mortality risk could be identified through gut microbiota analysis. Modulation therapy studies are warranted.
Results of Primary Treatment and Salvage Treatment in the Management of Patients with Non-Squamous Cell Malignant Tumors of the Sinonasal Region: Single Institution Experience
Authors: Kacorzyk Urszula , Kentnowski Marek , Szymczyk Cezary , Chmielik Ewa , Bobek-Billewicz Barbara , Składowski Krzysztof , Rutkowski Tomasz Wojciech
Published: March 2023

Non-squamous cell carcinoma-related malignant sinonasal tract tumors (non-SCC MSTT) are rare and diverse malignancies. In this study, we report our experience in the management of this group of patients. The treatment outcome has been presented, involving both primary treatment and salvage approaches. Data from 61 patients treated radically due to non-SCC MSTT between 2000 and 2016 at the National Cancer Research Institute, Gliwice branch, were analyzed. The group consisted of the following pathological subtypes of MSTT: adenoid cystic carcinoma (ACC), undifferentiated sinonasal carcinoma (USC), sarcoma, olfactory neuroblastoma (ONB), adenocarcinoma, small cell neuroendocrine carcinoma (SNC), mucoepidermic carcinoma (MEC), and acinic cell carcinoma, which were found in nineteen (31%), seventeen (28%), seven (11.5%), seven (11.5%), five (8%), three (5%), two (3%) and one (2%) of patients, respectively. There were 28 (46%) males and 33 (54%) females at the median age of 51 years. Maxilla was the primary tumor localization followed by the nasal cavity and ethmoid sinus in thirty-one (51%), twenty (32.5%), and seven (11.5%) patients, respectively. In 46 (74%) patients, an advanced tumor stage (T3 or T4) was diagnosed. Primary nodal involvement (N) was found in three (5%) cases, and all patients underwent radical treatment. The combined treatment consisted of surgery and radiotherapy (RT) and was given to 52 (85%) patients. The probabilities of overall survival (OS), locoregional control (LRC), metastases-free survival (MFS), and disease-free survival (DFS) were assessed in pathological subtypes and grouped together, along with the ratio and effectiveness of salvage. Locoregional treatment failure was seen in 21 (34%) patients. Salvage treatment was performed in fifteen (71%) patients and was effective in nine (60%) cases. There was a significant difference in OS between patients who underwent salvage and those who did not (median: 40 months vs. 7 months, p = 0.01). In the group of patients who underwent salvage, OS was significantly longer when the procedure was effective (median: 80.5 months) than if it failed (median: 20.5 months), p < 0.0001. OS in patients after effective salvage was the same as in patients who were primary cured (median: 80.5 months vs. 88 months, p = 0.8). Distant metastases developed in ten (16%) patients. Five and ten year LRC, MFS, DFS, and OS were 69%, 83%, 60%, 70%, and 58%, 83%, 47%, 49%, respectively. The best treatment results were observed for patients with adenocarcinoma and sarcoma, while USC gave the poorest results in our set of patients. In this study, we indicate that salvage is possible in most patients with non-SCC MSTT with locoregional failure and that it may significantly prolong their overall survival.
Chronic Pain and Regional Anesthesia: A Call to Action!
Authors: De Cassai Alessandro , Geraldini Federico
Published: March 2023

Chronic pain is an unwanted companion in the lives of millions of people worldwide, and findings show that more than one in fiveadults in America experience chronic pain [...]
Risk Factors for Hypoparathyroidism after Thyroid Surgery: A Single-Center Study
Authors: Privitera Francesca , Centonze Danilo , La Vignera Sandro , Condorelli Rosita Angela , Distefano Costanza , Gioco Rossella , Civit Alba Ilari , Riccioli Giordana , Stella Ludovica , Cavallaro Andrea , Cannizzaro Matteo Angelo , Veroux Massimiliano
Published: March 2023

Background: Hypoparathyroidism is one of the most common complications after thyroidectomy. This study evaluated the incidence and potential risk factors for postoperative hypoparathyroidism after thyroid surgical procedures in a single high-volume center. Methods: In this retrospective study, in all patients undergoing thyroid surgery from 2018 to 2021, a 6 h postoperative parathyroid hormone level (PTH) was evaluated. Patients were divided into two groups based on 6 h postoperative PTH levels (≤12 and >12 pg/mL). Results: A total of 734 patients were enrolled in this study. Most patients (702, 95.6%) underwent a total thyroidectomy, while 32 patients underwent a lobectomy (4.4%). A total of 230 patients (31.3%) had a postoperative PTH level of <12 pg/mL. Postoperative temporary hypoparathyroidism was more frequently associated with female sex, age < 40 y, neck dissection, the yield of lymph node dissection, and incidental parathyroidectomy. Incidental parathyroidectomy was reported in 122 patients (16.6%) and was correlated with thyroid cancer and neck dissection. Conclusions: Young patients undergoing neck dissection and with incidental parathyroidectomy have the highest risk of postoperative hypoparathyroidism after thyroid surgery. However, incidental parathyroidectomy did not necessarily correlate with postoperative hypocalcemia, suggesting that the pathogenesis of this complication is multifactorial and may include an impaired blood supply to parathyroid glands during thyroid surgery.
“The Pupillary (Hippus) Nystagmus”: A Possible Clinical Hallmark to Support the Diagnosis of Vestibular Migraine
Authors: Gufoni Mauro , Casani Augusto Pietro
Published: March 2023

(1) Background: Hippus (which in this paper will be called “Pupillary nystagmus”) is a well-known phenomenon which has never been related to any specific pathology, so much so that it can be considered physiological even in the normal subject, and is characterized by cycles of dilation and narrowing of the pupil under constant lighting conditions. The aim of this study is to verify the presence of pupillary nystagmus in a series of patients suffering from vestibular migraine. (2) Methods: 30 patients with dizziness suffering from vestibular migraine (VM), diagnosed according to the international criteria, were evaluated for the presence of pupillary nystagmus and compared with the results obtained in a group of 50 patients complaining of dizziness that was not migraine-related. (3) Results: Among the 30 VM patients, only two cases were found to be negative for pupillary nystagmus. Among the 50 non-migraineurs dizzy patients, three had pupillary nystagmus, while the remaining 47 did not. This resulted in a test sensitivity of 0.93% and a specificity of 0.94%. (4) Conclusion: we propose the consideration of the presence of pupillary nystagmus as an objective sign (present in the inter-critical phase) to be associated with the international diagnostic criteria for the diagnosis of vestibular migraine.
Review of Basic Research about Ossification of the Spinal Ligaments Focusing on Animal Models
Authors: Ikuta Masato , Kaito Takashi , Fujimori Takahito , Kitahara Takayuki , Furuichi Takuya , Bun Masayuki , Hirai Hiromasa , Ukon Yuichiro , Kanie Yuya , Takenaka Shota , Okada Seiji
Published: March 2023

Ossification of the posterior longitudinal ligament (OPLL) is a heterotopic ossification that may cause spinal cord compression. With the recent development of computed tomography (CT) imaging, it is known that patients with OPLL often have complications related to ossification of other spinal ligaments, and OPLL is now considered part of ossification of the spinal ligaments (OSL). OSL is known to be a multifactorial disease with associated genetic and environmental factors, but its pathophysiology has not been clearly elucidated. To elucidate the pathophysiology of OSL and develop novel therapeutic strategies, clinically relevant and validated animal models are needed. In this review, we focus on animal models that have been reported to date and discuss their pathophysiology and clinical relevance. The purpose of this review is to summarize the usefulness and problems of existing animal models and to help further the development of basic research on OSL.