bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum Metabolomics Combined With 16S rRNA Gene Sequencing to Analyze the Changes of Intestinal Flora in Rats With MI and the Intervention Effect of Fuling-Guizhi
Authors: Ye Yan , Liu Jianghua , Zheng Dan , Zeng Xiangfa , Zhou Zhenxiang , Han Lintao , Huang Ping , Zhang Fengyun , Wang Wusheng , Cheng Xuan , Huang Fang , Duan Bailu
Published: April 2023

Objective: This research aimed to assess the relationship between intestinal flora and metabolites in rats with myocardial ischemia (MI) and to offer a new perspective to elucidate the pathological mechanisms of MI and Fuling-Guizhi (FLGZ). Methods: Sprague-Dawley rats were divided into control check (CK), model (MX), propranolol (XDAZ), and FLGZ groups. Metabolomic analysis was performed by gas chromatography-mass spectrometry to obtain differential metabolites and 16S rRNA gene sequencing analysis to obtain differential intestinal flora and perform functional prediction. In addition, Pearson correlation analysis was conducted to reveal the correlation between different metabolites and altered intestinal flora. Results: By serum metabolomics, 14 different metabolites were identified, mainly involving amino acid, lipid, carbohydrate, and energy metabolic pathways. Linear discriminant analysis effect size analysis indicated that at the genus level, MI was associated with Phascolarctobacterium, Oscillospira, Allobaculum, and Ruminococcus, involving amino acids, carbohydrates, energy, lipids, cofactors and vitamins, other amino acids, terpenoids, and polyketides metabolism. Correlation analysis showed that ethanolamine, phosphate, L-glutamine, L-threonine, oxalic acid, and linoleic acid were closely associated with Phascolarctobacterium, Oscillospira, Allobaculum, and Ruminococcus. Conclusion: In conclusion, the results tentatively confirm that MI is closely tied to the dysregulation of metabolites and intestinal flora. FLGZ may affect amino acid, carbohydrate, lipid, and energy metabolism through Phascolarctobacterium, Oscillospira, Allobaculum, and Ruminococcus to attenuate inflammatory response, oxidative stress, and apoptosis.
Benchmarking Hotel Investment Risk: Differences Based on Types of Hotels
Authors: O’Neill John W. , Zhao Joann , Liu Peng , Caligiuri Michael D.
Published: April 2023

Commercial real estate (CRE) investment involves risk, and hotels are perceived as the riskiest CRE assets because of the high turnover of guest room occupants and are the most operation-intensive of all types of CRE properties. Furthermore, that risk may vary significantly across types of hotels based on different dimensions. The existing academic literature regarding CRE investment performance generally lacks such investigation of hotels at the establishment level. The purpose of the current study is to investigate the volatility of operating profit (risk) of different types of hotel assets. Using relative standard deviations of historical performance (gross operating profit [GOP]) to measure hotel risk, we examine various property characteristics and the extent to which they affect the volatility of GOP at the unit level from 2015 through 2020 of over 3,000 U.S. hotel properties. We find that different types of hotels have carried different levels of risk. Specifically, we find significant differences in risk based on hotel brand affiliation status, class, property type, location type, region in which the hotels are located, age of the hotels, size of the hotels, and their occupancy and average daily rate levels. This study provides practitioners and researchers with an understanding regarding the relationships between the risk of different types of hotels, and provides practitioners with information regarding risk and a benchmarking methodology that may be applied to evaluate risk to aid hotel investment decisions. Furthermore, we provide researchers with information regarding various hotel characteristics that may lead to relatively greater/lesser risk.
Unexpected, but consistent and pre-registered: Experimental evidence on interview language and Latino views of COVID-19
Authors: Pérez Efrén , HyunJeong Lee Jessica , Oaxaca Carrasco Ana L , Matthews Cole , Ritsema Madison
Published: April 2023

Much uncertainty remains about effective messaging to boost public support for COVID-19 mitigation efforts, especially among people of color. We investigate the relationship between interview language and expressed support for COVID-19 public health protocols among Latinos: America’s largest ethnic group. Prior work establishes that interview language shapes opinions by cognitively structuring which considerations people use to express attitudes. Yet other work suggests interview language shapes opinions by activating specific cultural norms associated with a tongue. We predicted that interviewing in Spanish (versus English) would boost support for COVID-19 protocols by activating pro-social norms known to be strongly associated with that language. We uncover null support for this prediction in a pre-registered experiment on bilingual Latino adults ( N = 1645). Instead, we find that Latinos assigned to interview in Spanish report weaker support for COVID-19 protocols, regardless of which cultural norms are primed. We discuss implications for COVID-19 attitudes in linguistically diverse polities.
When the Light Goes Out: Ignorance and Multiplicity in Teaching and Learning
Authors: Brohinsky Jais
Published: April 2023

Purpose

If education is the movement from dark to light, what does it mean for the light to go out? Following this question, this paper examines how conceptions of ignorance inform and are embedded in ideas of learning and pedagogy.

Design/Approach/Methods

Through historical and contemporary examples, I ask how an understanding of ignorance as absence frames some forms of teaching and learning, how ignorance might be understood differently, and how a different formulation of ignorance creates possibilities for imagining teaching and learning otherwise.

Findings

The framing of ignorance as absence articulates a distance between knowing and not knowing that defines what I call explicatory spacetime. This arrangement positions ignorance as a social problem and schooling as a solution aimed at closing the gap. I point to ways this framing persists today in research and practices that posit the acquisition of positive knowledge and the eradication of negative ignorance as a foremost educational responsibility.

Originality/Value

Engaging work across science and technology studies, psychoanalysis, and the philosophy of education, I argue that alternative conceptions of ignorance often maintain associations with absence. Building from this scholarship, I ask how reframing ignorance through multiplicity, that is, within a field of possibility out of which a thing called knowledge can cohere, offers a different framework and pedagogical arrangements. Finally, I explore how reframing ignorance in a spacetime of multiplicity opens possibilities for understanding and enacting pedagogical encounters.
Emerging Adulthood: Impacts of Adult Care on Education, Work, and Well-Being
Authors: Chy Somalis
Published: April 2023

An increasing number of young adults are providing unpaid care to an older or dependent family member or friend. However, we know little about the relationship between adult caregiving and emerging young adults. Using 2017 and 2019 data on Transition into Adulthood Supplement and the main Panel Study of Income Dynamics, this study examines relationships between adult care and nationally representative of emerging adult caregivers’, aged 17-28, work, education, and well-being and compares them to non-caregivers. Findings show that close to 10% of emerging adults are caregivers. Regression analyses observing these relationships contemporaneously and roughly three years later provide suggestive evidence of fewer work hours, lower educational attainment, and a significant decline in emotional well-being associated with adult care hours. These findings demonstrate the growing need for paid family leave and mental health counseling to alleviate stress and improve overall economic, education, and social emotional development for emerging young adults.
Vocal education in the context of intercultural communication: Experience of teaching chinese students
Authors: Nikolai Halyna , Kisiel Mirosław , Peng Yan
Published: April 2023

The article considers vocal education in an intercultural communication perspective, presents corresponding Polish research and experience of Chinese students training in Ukrainian universities. The result of the study is the idea of creating a kind of multi-aesthetic synthesis, where a specific association of vocal activities in different cultures will be made. One example of this is a project that will contain both the European vocal technique elements and the Chinese singing tradition in one performance. The article proves that internationalization processes in higher art education create the context of intercultural communication in the process of vocal training of Chinese students and a new trend emerges – research area of Chinese PhD students in Ukrainian universities. The studies of Chinese researchers conducted in Ukraine contribute to the achievement of the specified synthesis.
Value of D-Dimer in Risk Stratification for Thromboembolism in Patients With Atrial Fibrillation and Low CHA2DS2-VASc Score
Authors: Chen Po-Han , Schwade Mark , Sharma Gyanendra , Robinson Vincent J. B.
Published: April 2023

Atrial fibrillation (AF) can cause thrombi formation and subsequent emboli deposition in systemic arteries, leading to various organ ischemia and infarction. Anticoagulation therapy can reduce the risk of thrombus formation and embolization, and is initiated based on a patient’s risk score, which is frequently estimated with the CHA2DS2-VASc score. We present a case of thromboembolism (TE) where a low CHA2DS2-VASc score suggested a low-moderate risk of systemic embolization, but an elevated plasma D-dimer value prompted further investigation which revealed an intracardiac thrombus with renal embolism. The patient is a 63-year-old male with past medical history of hypertension and AF treated with ablation 2 years prior presenting with sharp right flank pain of 5-hour duration. Primary workup and imaging were unrevealing at the time, and a low CHA2DS2-VASc score was suggestive of aspirin therapy. However, an elevated D-dimer of 289 ng/mL and a transient increase in creatinine pointed to possible etiology of embolic origin. The diagnosis was confirmed with computed tomography (CT) with contrast and transesophageal echocardiogram, revealing renal infarcts and the source of the emboli, respectively. The patient was treated with heparin and transitioned to apixaban prior to discharge with full resolution of symptoms. Through this case, we wish to show D-dimer’s predictive value of TE, as well as its potential benefit in risk assessment in patients with AF.
An Extremely Rare Case of Prostate and Bladder Wall Involvement of Chronic Lymphocytic Leukemia
Authors: Thomas Mathew , Wazir Ali , Karikehalli Shridevi , Sandhu Michael , Poudel Aarati
Published: April 2023

Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults and is characterized by monoclonal proliferation of B-cell lymphocytes which are morphologically mature, but immunologically dysfunctional. The primary sites of disease involvement include peripheral blood, lymph nodes, spleen, and bone marrow. CLL can also present locally and aggressively at extranodal sites. We describe the case of a 74-year-old gentleman with multiple medical comorbidities who was Foley catheter–dependent at baseline for bladder outlet obstruction. He was detected to have Rai stage I CLL following an inguinal lymph node biopsy and was on regular outpatient surveillance. Later, he underwent a prostate biopsy for evaluation of hematuria, results of which were consistent with CLL involvement in the prostate and urinary bladder. The patient was started on single-agent ibrutinib, and demonstrated an excellent clinical response to bladder outlet obstruction. His long-term Foley catheter was discontinued within 5 days of ibrutinib therapy. Unfortunately, 1 year later, he had disease progression, and therapy was changed to a single-agent rituximab, to which he is responding well. Our case is unique as it brings up the first reported case of prostate and bladder wall CLL.
Forearm Pronation at Foot Contact: A Biomechanical Motion-Capture Analysis in High School and Professional Pitchers
Authors: Manzi Joseph E. , Dowling Brittany , Wang Zhaorui , Sudah Suleiman Y. , Quan Theodore , Moran Jay , McElheny Kathryn L. , Carr James B. , Gulotta Lawrence V. , Dines Joshua S.
Published: April 2023

Background:

It has previously been speculated that baseball pitchers who display excessive forearm pronation at foot contact (FC) have a higher propensity toward ulnar collateral ligament injury and subsequent surgery.

Purpose:

To evaluate the association between degree of forearm pronation/supination at FC and throwing arm kinetics in high school and professional pitchers, at both the individual (intrapitcher) and the group (interpitcher) level.

Study Design:

Descriptive laboratory study.

Methods:

High school (n = 41) and professional (n = 196) pitchers threw 8 to 12 fastballs while being assessed with a 3-dimensional motion-capture system (480 Hz). Pitchers at each playing level were divided into a supination or pronation subgroup depending on degree of forearm pronation at FC. Regression models were built to observe the relationship between forearm pronation at FC and kinetic and kinematic parameters of interest.

Results:

At both the individual and the group level of high school and professional pitchers, there was no significant correlation between forearm pronation at FC and elbow varus torque ( P min = .21). For every 10° increase in forearm pronation at FC in the individual high school pitcher, elbow flexion at FC decreased by 5°, whereas maximum elbow extension velocity was achieved 0.6% later in the pitch. In addition, elbow medial force increased by 4.1 N and elbow varus torque increased by 0.8 N·m for every 10° increase in forearm supination at FC. For every 10° increase in forearm supination in the individual professional pitcher, ball velocity increased by 0.5 m/s, shoulder external rotation at FC decreased by 11°, and elbow medial force decreased by 5.5 N.

Conclusion:

Supination- or pronation-predominant forearm motion during the pitch did not significantly differ between playing levels. Excessive forearm pronation at FC was not a significant risk factor for increased throwing arm kinetics for high school or professional pitchers. There was a weak positive association between forearm supination at FC and elbow varus torque in the individual high school pitcher. Ultimately, coaches and pitchers may be better served by redirecting their focus to other mechanical aspects of the pitch that may have stronger associations with injury risk implications as well as performance.
Tacrolimus Induced Leukoencephalopathy and Stroke-Like Symptoms: Case Report
Authors: Dopke Kelly M. , El Seblani Nader , Mercer Katherine , Naik Sunil , Mainali Gayatra , Paul Dustin
Published: April 2023

A 17-year-old female with sickle cell disease status post a recent stem cell transplant and on tacrolimus developed an acute expressive aphasia, dysphagia, and drooling. Brain MRI revealed diffuse restricted diffusion involving the bilateral corona radiata and areas of white matter in the right cerebral hemisphere most consistent with toxic leukoencephalopathy. Tacrolimus serum concentration was high at 19.3 ng/ml (ref 9-12 ng/ml) for which tacrolimus was discontinued. She was neurologically back at baseline 2 days later with the tacrolimus level improving to 8.2 ng/mL. Following discontinuation and the declining trend of her tacrolimus levels the patient returned to her neurologic baseline and was subsequently switched to mycophenolate mofetil for GVHD immunosuppression.
Self-Triage Use, Subsequent Healthcare Utilization, and Diagnoses: A Retrospective Study of Process and Clinical Outcomes Following Self-Triage and Self-Scheduling for Ear or Hearing Symptoms
Authors: North Frederick , Jensen Teresa B , Stroebel Robert J , Nelson Elissa M , Johnson Brenda J , Thompson Matthew C , Pecina Jennifer L , Crum Brian A
Published: April 2023

Background

Self-triage is becoming more widespread, but little is known about the people who are using online self-triage tools and their outcomes. For self-triage researchers, there are significant barriers to capturing subsequent healthcare outcomes. Our integrated healthcare system was able to capture subsequent healthcare utilization of individuals who used self-triage integrated with self-scheduling of provider visits.

Methods

We retrospectively examined healthcare utilization and diagnoses after patients had used self-triage and self-scheduling for ear or hearing symptoms. Outcomes and counts of office visits, telemedicine interactions, emergency department visits, and hospitalizations were captured. Diagnosis codes associated with subsequent provider visits were dichotomously categorized as being associated with ear or hearing concerns or not. Nonvisit care encounters of patient-initiated messages, nurse triage calls, and clinical communications were also captured.

Results

For 2168 self-triage uses, we were able to capture subsequent healthcare encounters within 7 days of the self-triage for 80.5% (1745/2168). In subsequent 1092 office visits with diagnoses, 83.1% (891/1092) of the uses were associated with relevant ear, nose and throat diagnoses. Only 0.24% (4/1662) of patients with captured outcomes were associated with a hospitalization within 7 days. Self-triage resulted in a self-scheduled office visit in 7.2% (126/1745). Office visits resulting from a self-scheduled visit had significantly fewer combined non-visit care encounters per office visit (fewer combined nurse triage calls, patient messages, and clinical communication messages) than office visits that were not self-scheduled (−0.51; 95% CI, −0.72 to −0.29; P < .0001).

Conclusion

In an appropriate healthcare setting, self-triage outcomes can be captured in a high percentage of uses to examine for safety, patient adherence to recommendations, and efficiency of self-triage. With the ear or hearing self-triage, most uses had subsequent visit diagnoses relevant to ear or hearing, so most patients appeared to be selecting the appropriate self-triage pathway for their symptoms.
Investigation of Mechanical and Dynamic Mechanical Properties of Sisal, Jute and Banana Peduncle Fibre Composite Materials
Authors: Durai Praveen Nagarajan , Kumar B Senthil , Mahesh G , Lakshmanakanth P
Published: April 2023

In this research work, different blend ratios of sisal, jute, and banana peduncle fibre (BPF) nonwoven textiles were reinforced for composite manufacturing. Nine different types of composite samples were produced using the fibre reinforcements. The mechanical properties such as tensile, flexural, impact, water absorption and DMA were analysed per different ASTM standards. Results indicated that the jute-/BPF combination of 75%:25% as reinforcement significantly improved the composite material’s mechanical and functional properties (DMA, water absorption). The proper fibre blending ensured higher matrix resin reinforcement, interlaminar strength and compactness in the structure. The SEM analysis showed the internal fracture in the composite specimen.
Side Effects Comparison of Coronavirus Vaccines Among Healthcare Workers in Shoushtar, Iran
Authors: Nabizadeh Edris , Honarmandpour Fatemeh , Mashhadizade Rezvan , Honarmandpour Azam
Published: April 2023

Objective:

Vaccination is one of the effective ways to fight against COVID-19 disease. Various vaccines have been designed during the coronavirus pandemic. Each of the used vaccines has beneficial effects as well as side effects. Healthcare workers were among the first vaccinated persons against COVID-19 in different countries. The current study aims to compare the side effects of AstraZeneca, Sinopharm, Bharat, and Sputnik V on healthcare workers in Iran.

Methods:

This descriptive study was conducted from July 2021 to January 2022 on 1639 healthcare workers who received the COVID-19 vaccines. Data were collected using a checklist that contained questions related to systemic, local, and severe side effects of the vaccine. The collected data were analyzed using the Kruskal-Wallis, Chi-square, and trend chi-square. P < .05 was regarded as a significant statistical difference.

Results:

The most commonly injected vaccines were Sinopharm (41.80%), Sputnik V (36.65%), AstraZeneca (17.75%), and Bharat (3.80%), respectively. At least 37.5% of participants reported one complication. The most common side effects after 72 hours of the first and second doses were as follows: injection site pain, fatigue, fever, myalgia, headache, and chill. Overall complication rates were reported as follows: AstraZeneca (91.4%), Sputnik V (65.9%), Sinopharm (56.8%), and Bharat (98.4%). Bharat showed the highest overall side effects, while Sinopharm had the lowest overall side effects. Also, our results indicated that individuals with a previous history of positive COVID-19 infection had a higher rate of overall complications.

Conclusions:

The majority of participants did not show life-threatening side effects after the injection of 1 of the 4 studied vaccines. Since it was well accepted and tolerable by the participants, it can be used widely and safely against SARS-CoV-2.
(Re)emergence of Pūtaiao: Conceptualising Kaupapa Māori science
Authors: Moko-Painting (Ngāti Manu, Te Popoto, Ngāpuhi) Te Kahuratai , Hamley (Ngāti Rangi, Whanganui) Logan , Hikuroa (Ngāti Maniapoto, Tainui, TeArawa) Dan , Le Grice (Ngapuhi, TeRarawa) Jade , McAllister (Te Aitanga a Māhaki, Ngāti Porou) Tara , McLellan (Whakatōhea, Ngāi Te Rangi) Georgia , Parkinson (Ngāti Hine, Ngāti Patuwai, Whakatōhea) Hineatua , Renfrew (Te Rarawa, Ngāpuhi, Tainui) Larissa , Rewi (Ngāpuhi, Ngāti Hine) Sarah T
Published: April 2023

Overcoming the long-standing distrust of ‘research’ is especially challenging within the colonial structures of Western science. This article aspires to rise to this challenge by conceptualising Pūtaiao as a form of Indigenous research sovereignty. Grounded in Kaupapa Māori Theory, Pūtaiao is envisioned as a Kaupapa Māori way of doing science in which Indigenous leadership is imperative. It incorporates Māori ways of knowing, being, and doing when undertaking scientific research. An essential element of Pūtaiao is setting a decolonising agenda, drawing from both Kaupapa Māori Theory and Indigenous methodologies. Accordingly, this centres the epistemology, ontology, axiology and positionality of researchers in all research, which informs their research standpoint. This approach speaks back to ontological framings of Western scientific research that restrict Indigenous ways of researching in the scientific academy. Furthermore, Pūtaiao offers tools and language to critique the academic disciplines of Western science which are a colonial construct within the global colonising agenda. As such, the theoretical search for Indigenous science(s) and Indigenising agendas explore the dialogical relationship between both knowledge systems – Kaupapa Māori science and Western science. This relationship necessitates setting a decolonising agenda before an Indigenising agenda can be realised, whereby they are mutually beneficial rather than mutually exclusive. This article is an affirmation of the work and discourse of Indigenous scientists. In this way, Pūtaiao becomes a pathway for asserting Indigenous sovereignty over and redefining scientific research for future generations of Māori and Indigenous researchers.
Calcioancylite-(La), (La,Ca)2(CO3)2(OH,H2O)2, a new member of the ancylite group from Gejiu nepheline syenite, Yunnan Province, China
Authors: Wang Yanjuan , Gu Xiangping , Dong Guochen , Hou Zengqian , Nestola Fabrizio , Yang Zhusen , Fan Guang , Wang Yufei , Qu Kai
Published: April 2023

Calcioancylite-(La), ideally (La,Ca)2(CO3)2(OH,H2O)2, has been discovered from nepheline syenite of the Gejiu alkaline complex in the Honghe Hani and Yi Autonomous Prefecture, Yunnan Province, China. The mineral occurs as aggregates of subhedral grains, and the size of single crystals varies between 5–20 μm. Calcioancylite-(La) is colourless to pale pinkish grey and has transparent to translucent lustre. It is brittle with a Mohs hardness of 4. The calculated density is 4.324 g/cm3. The mineral is biaxial (−), with α =1.662, β = 1.730, γ = 1.771, 2Vmeas. = 70°(1) and 2Vcalc. = 73°. Electron microprobe analysis for holotype material yielded an empirical formula of (La0.58Ce0.55Pr0.14Nd0.10Ca0.39Sr0.20K0.04)Σ2.00(CO3)2[(OH)1.25F0.06⋅0.69H2O]Σ2.00. Calcioancylite-(La) is orthorhombic, with space group Pmcn, a = 5.0253(3) Å, b = 8.5152(6) Å, c = 7.2717(6) Å, V = 311.17(4) Å3 and Z = 2. By using single-crystal X-ray diffraction, the crystal structure has been determined and refined to a final R1 = 0.0652 on the basis of 347 independent reflections (I > 2σ). The seven strongest powder X-ray diffraction lines [d in Å (I) (hkl)] are: 2.334 (100) (013), 2.970 (80) (121), 4.334 (75) (110), 3.678 (68) (111), 2.517 (55) (200), 2.647 (47) (031) and 2.077 (44) (221). Calcioancylite-(La) is the La-analogue of calcioancylite-(Ce) and is a new member of ancylite-group minerals. The mineral and its name have been approved by the Commission on New Minerals, Nomenclature and Classification of the International Mineralogical Association (IMA2021-090).
Bystrite, Na7Ca(Al6Si6O24)S52–Cl–: formula redefinition and relationships with other four-layer cancrinite-group minerals
Authors: Chukanov Nikita V. , Sapozhnikov Anatoly N. , Kaneva Ekaterina V. , Varlamov Dmitry A. , Vigasina Marina F.
Published: April 2023

Bystrite is redefined as a four-layer cancrinite-group mineral with the four-layer Losod-type framework and the end-member formula Na7Ca(Al6Si6O24)S52–Cl–. The mineral is known only at the Malo–Bystrinskoe gem lazurite deposit, Baikal Lake area, Siberia, Russia. The associated minerals are calcite, lazurite, sodalite, fluorapatite, phlogopite, diopside, dolomite and plagioclase. Bystrite is brittle, with the Mohs hardness of 5 and distinct cleavage on {10$\bar{1}$0}. The yellow colour of bystrite is due to the presence of S52– anions occurring in Losod (LOS) cages of the aluminosilicate framework with the ABAC stacking sequence. Measured and calculated density is, respectively, 2.43(1) and 2.412 g cm–3 for the holotype and 2.42(1) and 2.428 g cm–3 for the cotype sample. Bystrite is uniaxial (+), ɛ = 1.660(2) and ω = 1.584(2). The mineral was characterised by infrared and Raman spectra. The empirical formulae of the holotype and cotype samples are Na6.97K0.04Ca0.98(Si6.03Al5.97O24)(S52–)0.93[(SO42–)0.15Cl0.83] and Na6.75K0.04Ca1.11(Si6.09Al5.91O24)(S52–)1.04[(HS–)0.17Cl0.85], respectively. Bystrite is trigonal, space group P31c. The unit-cell parameters are: a = 12.8527(6) Å, c = 10.6907(5) Å, V = 1529.4(1) Å3 and Z = 2. The strongest lines of the powder X-ray diffraction pattern [d, Å (I, %) (hkl)] are: 4.821 (32) (102), 3.915 (38) (211), 3.712 (100) (300), 3.307 (50) (212), 2.782 (18) (400), 2.692 (22) (401), 2.673 (30) (004) and 2.468 (23) (402). Isomorphism and genesis of bystrite-type minerals is discussed. Bystrite and its K,HS-analogue sulfhydrylbystrite, Na5K2Ca(Al6Si6O24)S52–(HS)–, are indicators of highly reducing conditions.
Cytokine-targeted therapies for asthma and COPD
Authors: Schleich Florence , Bougard Nicolas , Moermans Catherine , Sabbe Mare , Louis Renaud
Published: April 2023

Asthma affects over 300 million people worldwide and its prevalence is increasing. COPD is the third leading cause of death globally. Asthma and COPD are complex inflammatory diseases of the airways in which impaired host defences lead to increased susceptibility to pathogens, pollutants and allergens. There is a constant interplay between host and the environment. Environmental exposures can alter the lung microbiome and influence the development of sensitisation by disrupting normal immunoregulation. The underlying airway inflammation in severe asthma is heterogeneous, with upregulation of type 2 cytokines in most cases but increased neutrophilic inflammation and activated T-helper 17 mediated immunity in others. COPD may also comprise several different phentoypes that are driven by different molecular mechanisms or endotypes. This disease heterogeneity is affected by comorbidities, treatments and environmental exposures. Recent intervention trials have shed light on the pathways beyond type 2 inflammation that can lead to beneficial outcomesversuspotentially deleterious effects. We have made a great deal of progress over the last 10 years in terms of immunology and the pathophysiology of asthma and this has led to the development of novel treatments and major improvements in severe asthma outcomes. In COPD, however, no targeted treatments have demonstrated great improvements. This article reviews the mechanism of action and efficacy of the available biologics in asthma and COPD.
Post-tuberculosis lung impairment: systematic review and meta-analysis of spirometry data from 14 621 people
Authors: Ivanova Olena , Hoffmann Verena Sophia , Lange Christoph , Hoelscher Michael , Rachow Andrea
Published: April 2023

Background

A substantial proportion of tuberculosis patients remain with pulmonary symptoms and reduced physical capacity despite successful treatment. We performed a systematic review to analyse the burden of post-tuberculosis lung impairment measured by lung function testing.

Methods

We searched the PubMed database for articles published between database inception and November 2020 and performed meta-analyses to estimate the prevalence, type and severity of lung impairment among drug-susceptible and multidrug-resistant tuberculosis survivors. Methodological quality of included studies was assessed using the Newcastle–Ottawa scale.

Results

54 articles were included in this review. For subjects with former drug-susceptible tuberculosis, the combined estimated mean was 76.6% (95% CI 71.6–81.6) of predicted for forced expiratory volume in 1 s (FEV1) and 81.8% (95% CI 77.4–86.2) for forced vital capacity (FVC). In former patients with multidrug-resistant tuberculosis, it was 65.9% (95% CI 57.1–74.7) for FEV1and 76.0% (95% CI 66.3–85.8) for FVC, respectively. The analysis of impairment types in former patients with drug-susceptible and multidrug-resistant tuberculosis showed that 22.0%versus19.0% had obstructive, 23.0%versus22.0% restrictive and 15.0%versus43.0% had mixed impairment type, respectively. In the majority of studies, at least 10–15% of tuberculosis survivors had severe lung impairment.

Conclusions

This systematic review showed long-term abnormal spirometry results in a significant proportion of tuberculosis survivors.
Development of revised ResNet-50 for diabetic retinopathy detection
Authors: Lin Chun-Ling , Wu Kun-Chi
Published: April 2023

Background

Diabetic retinopathy (DR) produces bleeding, exudation, and new blood vessel formation conditions. DR can damage the retinal blood vessels and cause vision loss or even blindness. If DR is detected early, ophthalmologists can use lasers to create tiny burns around the retinal tears to inhibit bleeding and prevent the formation of new blood vessels, in order to prevent deterioration of the disease. The rapid improvement of deep learning has made image recognition an effective technology; it can avoid misjudgments caused by different doctors’ evaluations and help doctors to predict the condition quickly. The aim of this paper is to adopt visualization and preprocessing in the ResNet-50 model to improve module calibration, to enable the model to predict DR accurately.

Results

This study compared the performance of the proposed method with other common CNNs models (Xception, AlexNet, VggNet-s, VggNet-16 and ResNet-50). In examining said models, the results alluded to an over-fitting phenomenon, and the outcome of the work demonstrates that the performance of the revised ResNet-50 (Train accuracy: 0.8395 and Test accuracy: 0.7432) is better than other common CNNs (that is, the revised structure of ResNet-50 could avoid the overfitting problem, decease the loss value, and reduce the fluctuation problem).

Conclusions

This study proposed two approaches to designing the DR grading system: a standard operation procedure (SOP) for preprocessing the fundus image, and a revised structure of ResNet-50, including an adaptive learning rating to adjust the weight of layers, regularization and change the structure of ResNet-50, which was selected for its suitable features. It is worth noting that the purpose of this study was not to design the most accurate DR screening network, but to demonstrate the effect of the SOP of DR and the visualization of the revised ResNet-50 model. The results provided an insight to revise the structure of CNNs using the visualization tool.
Global transcriptomic analysis of Francisella tularensis SchuS4 differentially expressed genes in response to doxycycline or ciprofloxacin exposure
Authors: Zaide Galia , Cohen-Gihon Inbar , Shifman Ohad , Israeli Ofir , Aftalion Moshe , Maoz Sharon , Chitlaru Theodor , Ber Raphael , Zvi Anat , Steinberger-Levy Ida
Published: April 2023

Objective

As part of a research aiming at presenting an alternative approach for rapid determination of antimicrobial susceptibility by quantification of changes in expression levels of specific marker genes and gene sets, cultures of the virulent bacterial strain Francisella tularensis SchuS4 were grown in the presence of inhibitory/sub-inhibitory concentrations of either ciprofloxacin or doxycycline and their transcriptomic profiles were elucidated using differential expression analysis followed by functional annotation.

Data description

RNA sequencing was performed to identify differentially expressed genes (DEGs) in response to exposure of F. tularensis SchuS4 to either ciprofloxacin or doxycycline, the antibiotics of choice for Tularemia therapy. Accordingly, RNA samples were collected 2 h post antibiotic exposure and subjected to RNA sequence analysis. Transcriptomic quantification of RNA representing duplicated samples generated highly similar gene expression data. Exposure to sub-inhibitory concentration [0.5 x MIC (minimal inhibitory concentration)] of doxycycline or ciprofloxacin modulated the expression of 237 or 8 genes, respectively, while exposure to an inhibitory concentration (1 x MIC) resulted in the modulation of 583 or 234 genes, respectively. Amongst the genes modulated upon doxycycline exposure upregulation of 31 genes encoding for translation-functions could be distinguished, as well as downregulation of 14 genes encoding for functions involved in DNA transcription and repair. Ciprofloxacin exposure impacted differently the RNA sequence profile of the pathogen, resulting in upregulation of 27 genes encoding mainly DNA replication and repair functions, transmembrane transporters and molecular chaperons. In addition, 15 downregulated genes were involved in translation processes.
Genomic insight of sulfate reducing bacterial genus Desulfofaba reveals their metabolic versatility in biogeochemical cycling
Authors: Gao Ping , Zhang Xiaoting , Huang Xiaomei , Chen Zhiyi , Marietou Angeliki , Holmkvist Lars , Qu Lingyun , Finster Kai , Gong Xianzhe
Published: April 2023

Background

Sulfate-reducing bacteria (SRB) drive the ocean sulfur and carbon cycling. They constitute a diverse phylogenetic and physiological group and are widely distributed in anoxic marine environments. From a physiological viewpoint, SRB’s can be categorized as complete or incomplete oxidizers, meaning that they either oxidize their carbon substrate completely to CO2 or to a stoichiometric mix of CO2 and acetate. Members of Desulfofabaceae family are incomplete oxidizers, and within that family, Desulfofaba is the only genus with three isolates that are classified into three species. Previous physiological experiments revealed their capability of respiring oxygen.

Results

Here, we sequenced the genomes of three isolates in Desulfofaba genus and reported on a genomic comparison of the three species to reveal their metabolic potentials. Based on their genomic contents, they all could oxidize propionate to acetate and CO2. We confirmed their phylogenetic position as incomplete oxidizers based on dissimilatory sulfate reductase (DsrAB) phylogeny. We found the complete pathway for dissimilatory sulfate reduction, but also different key genes for nitrogen cycling, including nitrogen fixation, assimilatory nitrate/nitrite reduction, and hydroxylamine reduction to nitrous oxide. Their genomes also contain genes that allow them to cope with oxygen and oxidative stress. They have genes that encode for diverse central metabolisms for utilizing different substrates with the potential for more strains to be isolated in the future, yet their distribution is limited.

Conclusions

Results based on marker gene search and curated metagenome assembled genomes search suggest a limited environmental distribution of this genus. Our results reveal a large metabolic versatility within the Desulfofaba genus which establishes their importance in biogeochemical cycling of carbon in their respective habitats, as well as in the support of the entire microbial community through releasing easily degraded organic matters.
Gastroesophageal reflux disease symptoms and associated factors among university students in Amhara region, Ethiopia, 2021: a cross-sectional study
Authors: Belete Mekonnen , Tesfaye Winta , Akalu Yonas , Adane Adugnaw , Yeshaw Yigizie
Published: April 2023

Introduction

Gastroesophageal reflux disease (GERD) symptom is a relapsing chronic medical condition resulting from the reflux of gastric acid contents into the esophagus and throat or mouth. It interferes with social functioning, sleep, productivity, and quality of life. Despite this, the magnitude of GERD symptoms is not known in Ethiopia. Therefore, this study was conducted to determine the prevalence and associated factors of GERD symptoms among university students in the Amhara national regional state.

Methods

An institutional-based cross-sectional study was employed in Amhara national regional state Universities, from April 1, 2021, to May 1, 2021. Eight hundred and forty-six students were included in the study. A stratified multistage sampling technique was employed. Data were collected by using a pretested self-administered questionnaire. Data were entered via Epi Data version 4.6.0.5 and analyzed by SPSS version-26 software. The bivariable and multivariable binary logistic regression analyses were used to determine the associated factors of GERD symptoms. The adjusted odds ratio (AOR) with a 95% confidence interval (CI) was calculated. Variables having a p-value of≤ 0.05 were considered statistically significant.

Results

The prevalence of GERD symptoms in this study was 32.1% (95% CI = 28.7–35.5%). Being in the age of 20–25 years (AOR = 1.74, 95%CI = 1.03–2.94), female (AOR = 1.67, 95% CI = 1.15–2.41), use of antipain (AOR = 2.47, 95% CI = 1.65–3.69) and soft drinks (AOR = 1.58, 95% CI = 1.13–2.20) were significantly associated with higher odds of GERD symptoms. Urban dwellers had less chance of having GERD symptoms (AOR = 0.67, 95% CI = 0.48–0.94).

Conclusion

Nearly one-third of university students are affected by GERD symptoms. Age, sex, residence, use of antipain, and consumption of soft drinks were significantly associated with GERD. Reducing modifiable risk factors such as antipain use and soft drink consumption among students is advisable to decrease the disease burden.
Maternal low and high hemoglobin concentrations and associations with adverse maternal and infant health outcomes: an updated global systematic review and meta-analysis
Authors: Young Melissa F. , Oaks Brietta M. , Rogers Hannah Paige , Tandon Sonia , Martorell Reynaldo , Dewey Kathryn G. , Wendt Amanda S.
Published: April 2023

Background

Growing evidence suggests low and high maternal hemoglobin (Hb) concentrations may have adverse consequences for maternal and child health. There remain questions on specific Hb thresholds to define anemia and high Hb as well as how cutoffs may vary by anemia etiology and timing of assessment.

Methods

We conducted an updated systematic review (using PubMed and Cochrane Review) on low (< 110 g/L) and high (≥ 130 g/L) maternal Hb concentrations and associations with a range of maternal and infant health outcomes. We examined associations by timing of Hb assessment (preconception; first, second, and third trimesters, as well as at any time point in pregnancy), varying cutoffs used for defining low and high hemoglobin concentrations and performed stratified analyses by iron-deficiency anemia. We conducted meta-analyses to obtain odds ratios (OR) and 95% confidence intervals.

Results

The updated systematic review included 148 studies. Low maternal Hb at any time point in pregnancy was associated with: low birthweight, LBW (OR (95% CI) 1.28 (1.22–1.35)), very low birthweight, VLBW (2.15 (1.47–3.13)), preterm birth, PTB (1.35 (1.29–1.42)), small-for-gestational age, SGA (1.11 (1.02–1.19)), stillbirth 1.43 (1.24–1.65)), perinatal mortality (1.75 (1.28–2.39)), neonatal mortality (1.25 (1.16–1.34), postpartum hemorrhage (1.69 (1.45–1.97)), transfusion (3.68 (2.58–5.26)), pre-eclampsia (1.57 (1.23–2.01)), and prenatal depression (1.44 (1.24–1.68)). For maternal mortality, the OR was higher for Hb < 90 (4.83 (2.17–10.74)) than for Hb < 100 (2.87 (1.08–7.67)). High maternal Hb was associated with: VLBW (1.35 (1.16–1.57)), PTB (1.12 (1.00-1.25)), SGA (1.17 (1.09–1.25)), stillbirth (1.32 (1.09–1.60)), maternal mortality (2.01 (1.12–3.61)), gestational diabetes (1.71 (1.19–2.46)), and pre-eclampsia (1.34 (1.16–1.56)). Stronger associations were noted earlier in pregnancy for low Hb and adverse birth outcomes while the role of timing of high Hb was inconsistent. Lower Hb cutoffs were associated with greater odds of poor outcomes; for high Hb, data were too limited to identify patterns. Information on anemia etiology was limited; relationships did not vary by iron-deficiency anemia.

Conclusion

Both low and high maternal Hb concentrations during pregnancy are strong predictors of adverse maternal and infant health outcomes. Additional research is needed to establish healthy reference ranges and design effective interventions to optimize maternal Hb during pregnancy.
Increasing health facility childbirth in Ghana: the role of network and community norms
Authors: Cofie Leslie E. , Barrington Clare , Cope Kersten , LePrevost Catherine E. , Singh Kavita
Published: April 2023

Background

Reducing pregnancy-related deaths in Sub-Saharan Africa through increases in health facility births may be achieved by promoting community norms and network norms favoring health facility births. However, the process of how both norms shift attitudes and actions towards facility delivery is little studied. We examined the association of network and community norms with facility birth, following a quality improvement intervention to improve facility births in Ghana.

Methods

A 2015 mixed methods evaluation of a Maternal and Newborn Health Referral (MNHR) project in Ghana included a cross-sectional survey of women (N = 508), aged 15–49 years; in-depth interviews (IDIs) with mothers (n = 40), husbands (n = 20) and healthcare improvement collaborative leaders (n = 8); and focus group discussions (FGDs) with mothers-in-law (n = 4) and collaborative members (n = 7). Multivariable logistic regression was used to examine the association of network and community norms with facility birth. Thematic analysis of the qualitative data was conducted to explain this relationship.

Results

The network norm of perceived family approval of facility delivery (AOR: 5.54, CI: 1.65–18.57) and the community norm of perceived number of women in the community that deliver in a facility (AOR: 3.00, CI: 1.66–5.43) were independently associated with facility delivery. In qualitative IDIs and FGDs both norms were also collectively perceived as influencing facility delivery. However, network norms were more influential in women’s utilization of facility-based pregnancy-related care. Healthcare improvement collaboratives were important in swaying both network and community norms toward facility-based delivery by offering pregnancy-related health information, antenatal care, and support for facility delivery.

Conclusion

Quality improvement initiatives impact both community and network norms. To be most impactful in advancing facility-based pregnancy-related care, these initiatives should focus on highlighting the shifting trend toward facility delivery in rural communicates and promoting support for facility delivery among women’s personal networks.
Spatial heterogeneity of low-birthweight deliveries on the Kenyan coast
Authors: Musau Moses M. , Mwakio Stella , Amadi David , Nyaguara Amek , Bejon Philip , Berkley James A. , Snow Robert W. , Kamau Alice
Published: April 2023

Background

Understanding spatial variations in health outcomes is a fundamental component in the design of effective, efficient public health strategies. Here we analyse the spatial heterogeneity of low birthweight (LBW) hospital deliveries from a demographic surveillance site on the Kenyan coast.

Methods

A secondary data analysis on singleton livebirths that occurred between 2011 and 2021 within the rural areas of the Kilifi Health and demographic surveillance system (KHDSS) was undertaken. Individual-level data was aggregated at enumeration zone (EZ) and sub-location level to estimate the incidence of LBW adjusted for accessibility index using the Gravity model. Finally, spatial variations in LBW were assessed using Martin Kulldorf’s spatial scan statistic under Discrete Poisson distribution.

Results

Access adjusted LBW incidence was estimated as 87 per 1,000 person years in the under 1 population (95% CI: 80, 97) at the sub-location level similar to EZ. The adjusted incidence ranged from 35 to 159 per 1,000 person years in the under 1 population at sub-location level. There were six significant clusters identified at sub-location level and 17 at EZ level using the spatial scan statistic.

Conclusions

LBW is a significant health risk on the Kenya coast, possibly under-estimated from previous health information systems, and the risk of LBW is not homogenously distributed across areas served by the County hospital.
A novel c.64G > T (p.G22C) NR5A1 variant in a Chinese adolescent with 46,XY disorders of sex development: a case report
Authors: Zhang Dan , Wang Dajia , Tong Yajie , Li Mingyu , Meng Lingzhe , Song Qiutong , Xin Ying
Published: April 2023

Background

Adolescents with 46,XY disorders of sex development (DSD) face additional medical and psychological challenges. To optimize management and minimize hazards, correct and early clinical and molecular diagnosis is necessary.

Case presentation

We report a 13-year-old Chinese adolescent with absent Müllerian derivatives and suspected testis in the inguinal area. History, examinations, and assistant examinations were available for clinical diagnosis of 46,XY DSD. The subsequent targeting specific disease‐causing genes, comprising 360 endocrine disease-causing genes, was employed for molecular diagnosis. A novel variation in nuclear receptor subfamily 5 group A member 1 (NR5A1) [c.64G > T (p.G22C)] was identified in the patient. In vitro functional analyses of the novel variant suggested no impairment to NR5A1 mRNA or protein expression relative to wild-type, and immunofluorescence confirmed similar localization of NR5A1 mutant to the cell nucleus. However, we observed decreased DNA-binding affinity by the NR5A1 variant, while dual-luciferase reporter assays showed that the mutant effectively downregulated the transactivation capacity of anti-Müllerian hormone. We described a novel NR5A1 variant and demonstrated its adverse effects on the functional integrity of the NR5A1 protein resulting in serious impairment of its modulation of gonadal development.

Conclusions

This study adds one novel NR5A1 variant to the pool of pathogenic variants and enriches the adolescents of information available about the mutation spectrum of this gene in Chinese population.