bluuebunny/crossref_metadata_2025 huggingface dataset:
A screening study of the spatial distribution and cumulative toxicity of agricultural pesticides in the European Union’s waters
Authors: Pistocchi A. , Dorati C. , Galimberti F. , Udias A. , Bopp S. , D’Andrimont R. , Catarino R. , Schaefer R. B.
Published: April 2023

Pesticides can be an important stressor to aquatic ecosystems, and their use is strictly regulated in the European Union (EU). However, data on the use of pesticides are rather limited and poorly available, and monitoring is often insufficient to characterize their actual exposure and impact. The aim of the work presented here is to harness the limited data available and assess, for the first time, the distribution of concentrations and toxicity of 148 pesticide active substances (AS) for the whole EU. Starting from available estimates of pesticide use in agriculture and a simple screening-level model of their fate and transport, we quantify pesticide concentrations in soil and water. A comparison with monitoring data shows that predicted water concentrations are in plausible orders of magnitude, hence the model can be regarded as a first-approximation representation of the distribution of pesticides in the environment. The toxicity of individual pesticide active substances (AS) is characterized by their concentrations divided by the respective no observed effect concentrations (NOEC) for aquatic organisms, which represents the “toxic units” (TU) of each AS. The cumulative toxicity of pesticides in soils and streams of the EU is obtained by summing the TU of individual AS. We estimate that the toxicity of individual AS is generally well below 0.1 TU, indicating relatively safe environmental exposure. However, the cumulative toxicity of a mixture of AS can exceed 0.1 toxic units (TU) for more than 27% of the length of the EU’s stream network, and 1 TU for more than 4%. The cumulative toxicity at a given location is driven by only a handful of AS, but these differ from site to site reflecting the variability of pesticide use. Still, we estimate that only about 20 AS out of 148 appear among the top contributors to cumulative toxicity in most cases. While our assessment suggests a relatively widespread risk due to pesticide pollution, it also points to the important limitations concerning knowledge of pesticide use and monitoring of pesticide occurrence in the environment. These limitations need to be addressed in order to evaluate more accurately the effectiveness of EU pesticide policies. The assessment represents a proof-of-concept of a method that can be applied in support of the monitoring of pesticide policies implementation in the EU and elsewhere, once pesticide use can be estimated.
Vitamin A deficiency and vitamin A supplementation affect innate and T cell immune responses to rotavirus A infection in a conventional sow model
Authors: Chepngeno Juliet , Amimo Joshua O. , Michael Husheem , Raev Sergei A. , Jung Kwonil , Lee Marcia V. , Damtie Debasu , Omwando Alfred , Vlasova Anastasia N. , Saif Linda J.
Published: April 2023

Rotavirus A (RVA) causes ~200,000 diarrheal deaths annually in children <5yrs, mostly in low- and middle-income countries. Risk factors include nutritional status, social factors, breastfeeding status, and immunodeficiency. We evaluated the effects of vitamin A (VA) deficiency/VA supplementation and RVA exposure (anamnestic) on innate and T cell immune responses in RVA seropositive pregnant and lactating sows and passive protection of their piglets post-RVA challenge. Sows were fed VA deficient (VAD) or sufficient (VAS) diets starting at gestation day (GD)30. A subset of VAD sows received VA supplementation from GD|76 (30,000IU/day, VAD+VA). Sows (6 groups) were inoculated with porcine RVA G5P[7] (OSU strain) or Minimal Essential Medium (mock) at GD~90: VAD+RVA; VAS+RVA; VAD+VA+RVA; VAD-mock; VAS-mock; and VAD+VA-mock. Blood, milk, and gut-associated tissues were collected from sows at several time points to examine innate [natural killer (NK), dendritic (DC) cells], T cell responses and changes in genes involved in the gut-mammary gland (MG)-immunological axis trafficking. Clinical signs of RVA were evaluated post inoculation of sows and post-challenge of piglets. We observed decreased frequencies of NK cells, total and MHCII+ plasmacytoid DCs, conventional DCs, CD103+ DCs and CD4+/CD8+ and T regulatory cells (Tregs) and NK cell activity in VAD+RVA sows. Polymeric Ig receptor and retinoic acid receptor alpha (RARα) genes were downregulated in mesenteric lymph nodes and ileum of VAD+RVA sows. Interestingly, RVA-specific IFN-γ producing CD4+/CD8+ T cells were increased in VAD-Mock sows, coinciding with increased IL-22 suggesting inflammation in these sows. VA supplementation to VAD+RVA sows restored frequencies of NK cells and pDCs, and NK activity, but not tissue cDCs and blood Tregs. In conclusion, similar to our recent observations of decreased B cell responses in VAD sows that led to decreased passive immune protection of their piglets, VAD impaired innate and T cell responses in sows, while VA supplementation to VAD sows restored some, but not all responses. Our data reiterate the importance of maintaining adequate VA levels and RVA immunization in pregnant and lactating mothers to achieve optimal immune responses, efficient function of the gut-MG-immune cell-axis and to improve passive protection of their piglets.
Cryptic diversity and virulence of Beauveria bassiana recovered from Lycorma delicatula (spotted lanternfly) in eastern Pennsylvania
Authors: Clifton Eric H. , Castrillo Louela A. , Jaronski Stefan T. , Hajek Ann E.
Published: April 2023

The entomopathogenic fungus Beauveria bassiana is cosmopolitan and known to infect a variety of sap-sucking pests like aphids, mealybugs, and scales in the order of Hemiptera. In Fall 2017, spotted lanternfly (SLF) adults killed by the fungal entomopathogen B. bassiana were found in Berks County, Pennsylvania. In 2018-2020 we collected SLF and nearby non-target insects killed by Beauveria spp. from 18 field sites in southeastern Pennsylvania. We identified 159 Beauveria isolates from SLF and six isolates from non-targets. Five isolates of B. bassiana and one isolate of B. brongniartii were identified from the non-targets. Based on sequence data from the nuclear B locus (Bloc) intergenic region, all the isolates from SLF were identified as B. bassiana, but there were 20 different strains within this species, grouped into two clades. Three B. bassiana strains (A, B, and L) were found in most field sites and were the most prevalent. Representative isolates for these three strains were used in laboratory bioassays and were compared to a commercial B. bassiana strain (GHA). Strain B was inferior to A, L, and GHA against nymphs; strains A and L had greater efficacy than B and GHA against adults. We also quantified conidial production on SLF cadavers. This paper discusses the diversity of these B. bassiana strains in SLF populations and implications for biological control of this abundant invasive.
The sources and transport pathways of sediment in the northern Ninety-east Ridge of the India Ocean over the last 35000 years
Authors: Cai Zhourong , Huang Qianru , Yin Zhengxin , Huang Xiaofeng , Chen Liang , Tang Meng
Published: April 2023

The Ninety-east Ridge (NER) is located in the southern Bay of Bengal in the northeast Indian Ocean and is composed of pelagic and hemipelagic sediments. In addition to contributions from marine biomass, the ridge also contains terrestrially sourced sedimentary material. However, considerable disagreement remains regarding the origin of these terrestrial materials and transport pathways. This paper discusses the collection of seafloor surface sediments and three sediment cores recovered from the northern region of the NER, as well as the analysis of clay minerals, Sr-Nd isotopes, and sediment grain size. The ages of the three core sediments are constrained by AMS 14C dating to better establish the source and transport pathways of the terrestrial materials within NER sediments over the past 35000 years. The research results show that the Qinghai-Tibet Plateau is the predominate source of terrigenous sedimentary material in the NER. In the plateau, the crustal materials were weathered and stripped and then transported to the Andaman Sea via the Irrawaddy River. From there, the material was transported westward by monsoon-driven circulation to the northernmost part of the NER before being transported to the south for final deposition. This transport mode has changed little over the past 35000 years. However, during the rapidly changing climate of the Younger Dryas (12.9~11.5 ka BP), there were some variations in the input amount, grain size, and Sr-Nd isotope value of the source material. The above conclusions are significant for re-evaluating the source of terrigenous sediments, the temporal and spatial changes in transport modes, and the sensitivity of the NER to climatic shifts.
The morphodynamic response of a gravel barrier to unimodal and bimodal storm wave conditions
Authors: Ions Kristian , Ozdemir Khan , Pender Douglas , Reeve D. E. , Karunarathna Harshinie
Published: April 2023

Gravel barrier beaches can offer natural protection to coastlines from adverse storm conditions. Understanding the morphodynamics of gravel barrier beaches is vital for the effective and sustainable management of these systems. Here, we use a synthetic dataset to investigate the morphodynamic response of the gravel barrier beach at Hurst Castle Spit, located on the Southwest coast of the United Kingdom, to both unimodal and bimodal storms. This spit is exposed to wind and swell waves propagating up the English Channel from the Southwest approaches and has suffered repeated storm erosion. The results are analyzed to identify the key drivers that govern the spatio-temporal gravel barrier morphodynamic responses to storms and to explore the morphodynamic states of the barrier. We found that the morphodynamic response of the barrier beach is strongly influenced by the combination of storm wave height and still water level. Further, the presence of swell waves can be a controlling factor in the barrier response.
Lower skeletal extension in Pleistocene Orbicella (Montastraea) corals than in their modern counterparts
Authors: Jordán-Garza Adán Guillermo , Blanchon Paul , Carricart-Ganivet Juan P. , Jordán-Dahlgren Eric
Published: April 2023

Introduction

Despite warmer conditions during the Last Interglacial, coral colonies of Orbicella were abundant and reached large sizes on many Caribbean reefs, including the extinct O. nancyi.

Methods

To explore variation in growth rates, we examined the yearly mean linear extension of growth bands in two fossil Orbicella species and compared them with two modern species of the same genus from shallow waters of the wider Caribbean.We measured the linear extension of corals exposed in a fossil reef and their modern counterparts, from both in situ colonies and coral slab X-rays.

Results

Few coral colonies showed autocorrelation or a linear trend on their linear-growth time series. A Bayesian ANOVA showed lower linear-extension rates of fossils compared to modern colonies and similar or lower than other fossil corals from the Pleistocene. Growth rates and growth form contribute significantly to the amount of tissue and size of coral colonies and can be a decisive trait for inter and intra specific competition.

Discussion

It is unlikely that temperature or interspecific competition explain modern coral extension rates and the low rates of the fossils data, which seem to be controlled instead by past habitat conditions.
Factors associated with loss to follow-up before and after treatment initiation among patients with tuberculosis: A 5-year observation in China
Authors: Jiang Youli , Chen Jingfang , Ying Meng , Liu Linlin , Li Min , Lu Shuihua , Li Zhihuan , Zhang Peize , Xie Qingyao , Liu Xuhui , Lu Hongzhou
Published: April 2023

Background

Loss to follow-up (LTFU) is a significant barrier to the completion of anti-tuberculosis (TB) treatment and a major predictor of TB-associated deaths. Currently, research on LTFU-related factors in China is both scarce and inconsistent.

Methods

We collected information from the TB observation database of the National Clinical Research Center for Infectious Diseases. The data of all patients who were documented as LTFU were assessed retrospectively and compared with those of patients who were not LTFU. Descriptive epidemiology and multivariable logistic regression analyses were conducted to identify the factors associated with LTFU.

Results

A total of 24,265 TB patients were included in the analysis. Of them, 3,046 were categorized as LTFU, including 678 who were lost before treatment initiation and 2,368 who were lost afterwards. The previous history of TB was independently associated with LTFU before treatment initiation. Having medical insurance, chronic hepatitis or cirrhosis, and providing an alternative contact were independent predictive factors for LTFU after treatment initiation.

Conclusion

Loss to follow-up is frequent in the management of patients with TB and can be predicted using patients’ treatment history, clinical characteristics, and socioeconomic factors. Our research illustrates the importance of early assessment and intervention after diagnosis. Targeted measures can improve patient engagement and ultimately treatment adherence, leading to better health outcomes and disease control.
Unravelling copper effect on the production of varietal thiols during Colombard and Gros Manseng grape juices fermentation by Saccharomyces cerevisiae
Authors: Dournes Gabriel , Dufourcq Thierry , Suc Lucas , Roland Aurélie , Mouret Jean-Roch
Published: April 2023

Nowadays the rapidly increasing organic vineyard management with the utilization of copper as sole fungal control pesticide against downy mildew raises once again the question of copper impact on varietal thiols in wine. For this purpose, Colombard and Gros Manseng grape juices were fermented under different copper levels (from 0.2 to 3.88  mg/l) to mimic the consequences in must of organic practices. The consumption of thiol precursors and the release of varietal thiols (both free and oxidized forms of 3-sulfanylhexanol and 3-sulfanylhexyl acetate) were monitored by LC–MS/MS. It was found that the highest copper level (3.6 and 3.88  mg/l for Colombard and Gros Manseng respectively) significantly increased yeast consumption of precursors (by 9.0 and 7.6% for Colombard and Gros Manseng respectively). For both grape varieties, free thiol content in wine significantly decreased (by 84 and 47% for Colombard and Gros Manseng respectively) with the increase of copper in the starting must as already described in the literature. However, the total thiol content produced throughout fermentation was constant regardless of copper conditions for the Colombard must, meaning that the effect of copper was only oxidative for this variety. Meanwhile, in Gros Manseng fermentation, the total thiol content increased along with copper content, resulting in an increase up to 90%; this suggests that copper may modify the regulation of the production pathways of varietal thiols, also underlining the key role of oxidation. These results complement our knowledge on copper effect during thiol-oriented fermentation and the importance of considering the total thiol production (reduced+oxidized) to better understand the effect of studied parameters and differenciate chemical from biological effects.
Microbial community succession in the intestine of mice with deep partial-thickness burns
Authors: Chen Li-Jian , Liu Yi , Yang Jing-Wen , Lin Yan , Hsu Clare , Zhang Kai-Kai , Liu Jia-Li , Li Jia-Hao , Li Xiu-Wen , Yang Jian-Zheng , Chen Long , Zeng Jia-Hao , Xie Xiao-Li , Xu Jing-Tao , Wang Qi
Published: April 2023

Introduction

Burn injury has been shown to lead to changes in the composition of the gut microbiome and cause other damage in patients. However, little is known about how the gut microbial community evolves in individuals who have recovered from burn injury.

Methods

In this study, we established a model of deep partial-thickness burn in mice and collected fecal samples at eight time points (pre-burn, 1, 3, 5, 7, 14, 21, and 28 days post-burn) for 16S rRNA amplification and high-throughput sequencing.

Results

The results of the sequencing were analyzed using measures of alpha diversity, and beta diversity and taxonomy. We observed that the richness of the gut microbiome declined from day 7 post-burn and that the principal component and microbial community structure varied over time. On day 28 after the burn, the microbiome composition largely returned to the pre-burn level, although day 5 was a turning point for change. Some probiotics, such as the Lachnospiraceae_NK4A136_group, decreased in composition after the burn but were restored in the later recovery period. In contrast, Proteobacteria showed an opposite trend, which is known to include potential pathogenic bacteria.

Conclusion

These findings demonstrate gut microbial dysbiosis after burn injury and provide new insights into the burn-related dysbiosis of the gut microbiome and strategies for improving the treatment of burn injury from the perspective of the microbiota.
Reinforcer value moderates the effects of prenatal alcohol exposure on learning and reversal
Authors: Chandrasekaran Jayapriya , Jacquez Belkis , Wilson Jennifer , Brigman Jonathan L.
Published: April 2023

Introduction

Fetal Alcohol Spectrum Disorders (FASD) are the leading cause of preventable developmental disability and are commonly characterized by alterations in executive function. Reversal learning tasks are reliable, cross-species methods for testing a frequently impaired aspect of executive control, behavioral flexibility. Pre-clinical studies commonly require the use of reinforcers to motivate animals to learn and perform the task. While there are several reinforcers available, the most commonly employed are solid (food pellets) and liquid (sweetened milk) rewards. Previous studies have examined the effects of different solid rewards or liquid dietary content on learning in instrumental responding and found that rodents on liquid reward with higher caloric content performed better with increased response and task acquisition rate. The influence of reinforcer type on reversal learning and how this interacts with developmental insults such as prenatal alcohol exposure (PAE) has not been explored.

Methods

We tested whether reinforcer type during learning or reversal would impact an established deficit in PAE mice.

Results

We found that all male and female mice on liquid reward, regardless of prenatal exposure were better motivated to learn task behaviors during pre-training. Consistent with previous findings, both male and female PAE mice and Saccharine control mice were able to learn the initial stimulus reward associations irrespective of the reinforcer type. During the initial reversal phase, male PAE mice that received pellet rewards exhibited maladaptive perseverative responding whereas male mice that received liquid rewards performed comparable to their control counterparts. Female PAE mice that received either reinforcer types did not exhibit any deficits on behavioral flexibility. Female saccharine control mice that received liquid, but not pellet, rewards showed increased perseverative responding during the early reversal phase.

Discussion

These data suggest that reinforcer type can have a major impact on motivation, and therefore performance, during reversal learning. Highly motivating rewards may mask behavioral deficits seen with more moderately sought rewards and gestational exposure to the non-caloric sweetener, saccharine, can impact behavior motivated by those reinforcers in a sex-dependent manner.
Circadian glucocorticoids throughout development
Authors: Lehmann Marianne , Haury Katharina , Oster Henrik , Astiz Mariana
Published: April 2023

Glucocorticoids (GCs) are essential drivers of mammalian tissue growth and maturation during one of the most critical developmental windows, the perinatal period. The developing circadian clock is shaped by maternal GCs. GC deficits, excess, or exposure at the wrong time of day leads to persisting effects later in life. During adulthood, GCs are one of the main hormonal outputs of the circadian system, peaking at the beginning of the active phase (i.e., the morning in humans and the evening in nocturnal rodents) and contributing to the coordination of complex functions such as energy metabolism and behavior, across the day. Our article discusses the current knowledge on the development of the circadian system with a focus on the role of GC rhythm. We explore the bidirectional interaction between GCs and clocks at the molecular and systemic levels, discuss the evidence of GC influence on the master clock in the suprachiasmatic nuclei (SCN) of the hypothalamus during development and in the adult system.
An ileal gastrointestinal stromal tumor misdiagnosed as pelvic metastases from rectal cancer: a case report
Authors: Ma Jun , Zhu Juan , Yu Shuihong , Zhou Chaoping , Duan Shuqiang , Zhang Yaming
Published: April 2023

With the advancement of imaging and pathological diagnostic methods, it is not uncommon to see synchronous gastrointestinal stromal tumors (GIST) and other primary cancers, the most common of which are synchronous gastric cancer and gastric GIST. However, synchronous advanced rectal cancer and high-risk GIST in the terminal ileum are extremely rare, and they are easily misdiagnosed as rectal cancer with pelvic metastases due to their special location near iliac vessels. Herein, we report a 55-year-old Chinese woman with rectal cancer. Preoperative imaging revealed a middle and lower rectal lesion with a right pelvic mass (considered possible metastasis from rectal cancer). Through multidisciplinary discussions, we suspected the possibility of rectal cancer synchronous with a GIST in the terminal ileum. Intraoperative exploration by laparoscopy revealed a terminal ileal mass with pelvic adhesion, a rectal mass with plasma membrane depression, and no abdominal or liver metastases. Laparoscopic radical proctectomy (DIXON) plus partial small bowel resection plus prophylactic loop ileostomy was performed, and the pathological report confirmed the coexistence of advanced rectal cancer and a high-risk ileal GIST. The patient was treated with the chemotherapy (CAPEOX regimen) plus targeted therapy(imatinib) after surgery, and no abnormalities were observed on the follow-up examination. Synchronous rectal cancer and ileal GIST are rare and easily misdiagnosed as a rectal cancer with pelvic metastases, and careful preoperative imaging analysis and prompt laparoscopic exploration are required to determine the diagnosis and prolong patient survival.
Stephen Massey: a career in visual neuroscience
Authors: Mills Stephen L. , Marshak David W.
Published: April 2023

This review is a memoir by Dr. Stephen C. Massey’s longtime collaborator, Dr. Stephen L. Mills, and written, for the most part, in the first person. It also serves as an introduction to the virtual festschrift to celebrate Dr. Massey’s retirement. and. The references cited here are only a few of the highlights of Dr. Massey’s distinguished career. A complete list is found here: https://pubmed.ncbi.nlm.nih.gov/?term=massey+sc+%28retina+or+photoreceptors%29&sort=date.
What is tongue-tie and does it interfere with breast-feeding? – a brief review
Authors: Borowitz Stephen M.
Published: April 2023

The most common symptom attributed to ankyloglossia is difficulty breast feeding due to poor latch, inefficient milk extraction and/or maternal nipple pain. During the past two decades, despite a declining birth rate, there has been a dramatic increase in the number of infants diagnosed with and treated for ankyloglossia in the United States, Canada and Australia. Despite a dramatic increase in the diagnosis and treatment of ankyloglossia in these countries, there remains no universally agreed upon definition of ankyloglossia and none of the published scoring systems have been rigorously validated. However ankyloglossia is defined, the majority of infants with ankyloglossia are asymptomatic. Perhaps, infants with ankyloglossia have a greater incidence of difficulty breast feeding. Lingual frenulotomy may decrease maternal pain and at least transiently improve the quality of breast feeding in some infants however no published studies take into account the fact that sucking and feeding are soothing to infants and the observed improvements immediately following frenulotomy may be a response to the pain associated with the procedure rather than a result of the procedure itself. While there are almost certainly some infants in whom tongue-tie interferes with breast-feeding, there is currently no good evidence lingual frenulotomy leads to longer duration of breast-feeding. Frenulotomy appears to be a generally safe procedure however there are reports of serious complications. Finally, there are no studies of long-term outcomes following frenulotomy during infancy and given traditional thinking that the lingual frenulum is a cord of connective tissue tethering the tongue to the floor of the mouth may be incorrect and the frenulum contains motor and sensory branches of the lingual nerve, the procedure may be less benign than previously thought.
Pediatric invasive fungal rhinosinusitis
Authors: Villamor Perla , Arango Valeria , Cortes Cristina , de la Torre Carlos
Published: April 2023

Pediatric invasive fungal rhinosinusitis (PIFR) is a rapidly progressive, potentially fatal disease. Previous medical literature demonstrates that its early diagnosis significantly reduces the risk of mortality in these patients. This study aims to present an updated clinical algorithm for optimized diagnosis and management of PIFR. A comprehensive review was conducted with only original, full-text articles published in English and Spanish from Cochrane Library, Pub-Med/MEDLINE, Embase, Scopus, and Google Scholar between January 2010 and June 2022. Relevant information was extracted and then integrated to develop a clinical algorithm for a proper diagnosis and management of PIFR.
Neonatal warming devices: What can be recommended for low-resource settings when skin-to-skin care is not feasible?
Authors: Kyokan Michiko , Rosa-Mangeret Flavia , Gani Matthieu , Pfister Riccardo E.
Published: April 2023

Hypothermia occurs frequently among clinically unstable neonates who are not suitable to place in skin-to-skin care. This study aims to explore the existing evidence on the effectiveness, usability, and affordability of neonatal warming devices when skin-to-skin care is not feasible in low-resource settings. To explore existing data, we searched for (1) systematic reviews as well as randomised and quasi-randomised controlled trials comparing the effectiveness of radiant warmers, conductive warmers, or incubators among neonates, (2) neonatal thermal care guidelines for the use of warming devices in low-resource settings and (3) technical specification and resource requirement of warming devices which are available in the market and certified medical device by the US Food and Drug Administration or with a CE marking. Seven studies met the inclusion criteria, two were systematic reviews comparing radiant warmers vs. incubators and heated water-filled mattresses vs. incubators, and five were randomised controlled trials comparing conductive thermal mattresses with phase-change materials vs. radiant warmers and low-cost cardboard incubator vs. standard incubator. There was no significant difference in effectiveness between devices except radiant warmers caused a statistically significant increase in insensible water loss. Seven guidelines covering the use of neonatal warming devices show no consensus about the choice of warming methods for clinically unstable neonates. The main warming devices currently available and intended for low-resource settings are radiant warmers, incubators, and conductive warmers with advantages and limitations in terms of characteristics and resource requirements. Some devices require consumables which need to be considered when making a purchase decision. As effectiveness is comparable between devices, specific requirements according to patients' characteristics, technical specification, and context suitability must play a primary role in the selection and purchasing decision of warming devices. In the delivery room, a radiant warmer allows fast access during a short period and will benefit numerous neonates. In the neonatal unit, warming mattresses are low-cost, effective, and low-electricity consumption devices. Finally, incubators are required for very premature infants to control insensible water losses, mainly during the first one to two weeks of life, mostly in referral centres.
Adverse events of PD-(L)1 inhibitors plus anti-VEGF(R) agents compared with PD-(L)1 inhibitors alone for cancer patients: a systematic review and meta-analysis
Authors: Tang Qiyu , Wu Dawei , Huang Huiyao , Fang Hong , Wu Ying , Liu Funan , Li Ning
Published: April 2023

Background: Anti-PD-(L)1 antibody monotherapy or in combination with VEGF(R) blockade has been applied widely for cancer treatment. Whether combination therapy increases irAEs still remains controversial.

Methods: A systematic review and meta-analysis comparing PD-(L)1 and VEGF(R) blockade combination therapy with PD-(L)1 inhibitors alone was performed. Phase II or III randomized clinical trials reporting irAEs or trAEs were included. The protocol was registered with PROSPERO, CRD42021287603.

Results: Overall, 77 articles were included in the meta-analysis. A total of 31 studies involving 8,638 participants were pooled and an incidence for PD-(L)1 inhibitor monotherapy with any grade and grade ≥3 irAEs of 0.25 (0.20, 0.32) and 0.06 (0.05, 0.07), respectively, were reported. Two studies with 863 participants pooled for PD-(L)1 and VEGF(R) blockade showed that an incidence of any grade and grade ≥3 irAEs were 0.47 (0.30, 0.65) and 0.11 (0.08, 0.16), respectively. Regarding pairwise comparisons for irAEs, only one study was included, indicating no significant difference between the two regimens in terms of colitis, hyperthyroidism, and hypothyroidism for any grade and grade ≥3, while there was a trend of higher incidence for any grade hyperthyroidism under the combination therapy. The incidence of reactive cutaneous capillary endothelial proliferation (RCCEP) was as high as 0.80 under camrelizumab monotherapy.

Conclusion: Total incidences of any grade and grade ≥3 irAEs were higher in the combination treatment group. Direct comparisons indicated no significant difference between the two regimens for any grade and grade ≥3 specific irAEs. RCCEP and thyroid disorders need to be paid attention to clinically. Moreover, trials with direct comparisons are needed and the safety profiles of the two regimens should be further explored. Exploration of the mechanism of action and regulatory management of adverse events should be enhanced.

Systematic Review Registration: https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=287603, identifier CRD42021287603
Scientometric analysis of lipid metabolism in breast neoplasm: 2012–2021
Authors: Lin Xiaobing , Yang Qiuping , Zheng Daitian , Tian Huiting , Chen Lingzhi , Wu Jinyao , Ji Zeqi , Chen Yexi , Li Zhiyang
Published: April 2023

Introduction: In recent years, more and more studies have proved that lipid metabolism plays an essential role in breast cancer’s proliferation and metastasisand also has a specific significance in predicting survival.

Methods: This paper collected data from 725 publications related to lipid metabolism in breast neoplasm from 2012 to 2021 through the Web of Science Core Collection database. Bibliometrix, VOSviewer, and CiteSpace were used for the scientometrics analysis of countries, institutions, journals, authors, keywords, etc.

Results: The number of documents published showed an increasing trend, with an average annual growth rate of 14.49%. The United States was the most productive country (n = 223, 30.76%). The journals with the largest number of publications are mostly from developed countries. Except for the retrieved topics, “lipid metabolism” (n = 272) and “breast cancer” (n = 175), the keywords that appeared most frequently were “expression” (n = 151), “fatty-acid synthase” (n = 78), “growth” (n = 72), “metabolism” (n = 67) and “cells“ (n = 66).

Discussion: These findings and summaries help reveal the current research status and clarify the hot spots in this field.
Latent space unsupervised semantic segmentation
Authors: Strommen Knut J. , Tørresen Jim , Côté-Allard Ulysse
Published: April 2023

The development of compact and energy-efficient wearable sensors has led to an increase in the availability of biosignals. To effectively and efficiently analyze continuously recorded and multidimensional time series at scale, the ability to perform meaningful unsupervised data segmentation is an auspicious target. A common way to achieve this is to identify change-points within the time series as the segmentation basis. However, traditional change-point detection algorithms often come with drawbacks, limiting their real-world applicability. Notably, they generally rely on the complete time series to be available and thus cannot be used for real-time applications. Another common limitation is that they poorly (or cannot) handle the segmentation of multidimensional time series. Consequently, the main contribution of this work is to propose a novel unsupervised segmentation algorithm for multidimensional time series named Latent Space Unsupervised Semantic Segmentation (LS-USS), which was designed to easily work with both online and batch data. Latent Space Unsupervised Semantic Segmentation addresses the challenge of multivariate change-point detection by utilizing an autoencoder to learn a 1-dimensional latent space on which change-point detection is then performed. To address the challenge of real-time time series segmentation, this work introduces the Local Threshold Extraction Algorithm (LTEA) and a “batch collapse” algorithm. The “batch collapse” algorithm enables Latent Space Unsupervised Semantic Segmentation to process streaming data by dividing it into manageable batches, while Local Threshold Extraction Algorithm is employed to detect change-points in the time series whenever the computed metric by Latent Space Unsupervised Semantic Segmentation exceeds a predefined threshold. By using these algorithms in combination, our approach is able to accurately segment time series data in real-time, making it well-suited for applications where timely detection of changes is critical. When evaluating Latent Space Unsupervised Semantic Segmentation on a variety of real-world datasets the Latent Space Unsupervised Semantic Segmentation systematically achieves equal or better performance than other state-of-the-art change-point detection algorithms it is compared to in both offline and real-time settings.
A mouthpart transcriptome for Spodoptera frugiperda adults: identification of candidate chemoreceptors and investigation of expression patterns
Authors: Dong Jun-Feng , Hu Zhen-Jie , Dong Bing-Xin , Tian Cai-Hong
Published: April 2023

Moth mouthparts, consisting of labial palps and proboscis, not only are the feeding device but also are chemosensory organs for the detection of chemical signals from surrounding environment. Up to now, the chemosensory systems in the mouthpart of moths are largely unknown. Here, we performed systematic analyses of the mouthpart transcriptome of adult Spodoptera frugiperda (Lepidoptera: Noctuidae), a notorious pest that spreads worldwide. A total of 48 chemoreceptors, including 29 odorant receptors (ORs), 9 gustatory receptors (GRs), and 10 ionotropic receptors (IRs), were annotated. Further phylogenetic analyses with these genes and homologs from other insect species determined that specific genes, including ORco, carbon dioxide receptors, pheromone receptor, IR co-receptors, and sugar receptors, were transcribed in the mouthpart of S. frugiperda adults. Subsequently, expression profiling in different chemosensory tissues demonstrated that the annotated ORs and IRs were mainly expressed in S. frugiperda antennae, but one IR was also highly expressed in the mouthparts. In comparison, SfruGRs were mainly expressed in the mouthparts, but 3 GRs were also highly expressed in the antennae or the legs. Further comparison of the mouthpart-biased chemoreceptors using RT-qPCR revealed that the expression of these genes varied significantly between labial palps and proboscises. This study provides the first large-scale description of chemoreceptors in the mouthpart of adult S. frugiperda and provides a foundation for further functional studies of chemoreceptors in the mouthpart of S. frugiperda as well as of other moth species.
Automated facial expression analysis of participants self-criticising via the two-chair technique: exploring facial behavioral markers of self-criticism
Authors: Halamová Júlia , Kanovský Martin , Brockington Guilherme , Strnádelová Bronislava
Published: April 2023

Introduction

As self-rating scales are prone to many measurement distortions, there is a growing call for more objective measures based on physiological or behavioural indicators. Self-criticism is one of the major transdiagnostic factor of all mental disorders therefore it is important to be able to distinguish what are the characteristic facial features of self-criticizing. To the best of our knowledge, there has been no automated facial emotion expression analysis of participants self-criticising via the two-chair technique. The aim of this study was to detect which action units of facial expressions were significantly more often present in participants performing self-criticism using the two-chair technique. The broader goal was to contribute to the scientific knowledge on objective behavioural descriptions of self-criticism and to provide an additional diagnostic means to the existing self-rating scales by exploring facial behavioral markers of self-criticism.

Methods

The non-clinical sample consisted of 80 participants (20 men and 60 women) aged 19 years to 57 years (M = 23.86; SD = 5.98). In the analysis we used iMotions’s Affectiva AFFDEX module (Version 8.1) to classify the participants’ actions units from the self-criticising videos. For the statistical analysis we used a multilevel model to account for the repeated-measures design.

Results

Based on the significant results the self-critical facial expression may therefore comprise the following action units: Dimpler, Lip Press, Eye Closure, Jaw Drop, and Outer Brow Raise, which are related to contempt, fear, and embarrassment or shame; and Eye Closure and Eye Widen (in rapid sequence Blink), which are a sign that highly negative stimuli are being emotionally processed.

Discussion

The research study need to be further analysed using clinical samples to compare the results.
Clarifying the relation between mechanistic explanations and reductionism
Authors: Couch Mark
Published: April 2023

The topic of mechanistic explanation in neuroscience has been a subject of recent discussion. There is a lot of interest in understanding what these explanations involve. Furthermore, there is disagreement about whether neurological mechanisms themselves should be viewed as reductionist in nature. In this paper I will explain how these two issues are related. I will, first, describe how mechanisms support a form of antireductionism. This is because the mechanisms that exist should be seen as involving part-whole relations, where the behavior of a whole is more than the sum of its parts. After this, I will consider mechanistic explanations and how they can be understood. While some people think the explanations concern existing entities in the world, I will argue that we can understand the explanations by viewing them in terms of arguments. Despite the fact that it is possible to understand mechanistic explanations in this manner, the antireductionist point remains.
Blood transcriptome analysis suggests an indirect molecular association of early life adversities and adult social anxiety disorder by immune-related signal transduction
Authors: Edelmann Susanne , Wiegand Ariane , Hentrich Thomas , Pasche Sarah , Schulze-Hentrich Julia Maria , Munk Matthias H. J. , Fallgatter Andreas J. , Kreifelts Benjamin , Nieratschker Vanessa
Published: April 2023

Social anxiety disorder (SAD) is a psychiatric disorder characterized by severe fear in social situations and avoidance of these. Multiple genetic as well as environmental factors contribute to the etiopathology of SAD. One of the main risk factors for SAD is stress, especially during early periods of life (early life adversity; ELA). ELA leads to structural and regulatory alterations contributing to disease vulnerability. This includes the dysregulation of the immune response. However, the molecular link between ELA and the risk for SAD in adulthood remains largely unclear. Evidence is emerging that long-lasting changes of gene expression patterns play an important role in the biological mechanisms linking ELA and SAD. Therefore, we conducted a transcriptome study of SAD and ELA performing RNA sequencing in peripheral blood samples. Analyzing differential gene expression between individuals suffering from SAD with high or low levels of ELA and healthy individuals with high or low levels of ELA, 13 significantly differentially expressed genes (DEGs) were identified with respect to SAD while no significant differences in expression were identified with respect to ELA. The most significantly expressed gene was MAPK3 (p = 0.003) being upregulated in the SAD group compared to control individuals. In contrary, weighted gene co-expression network analysis (WGCNA) identified only modules significantly associated with ELA (p ≤ 0.05), not with SAD. Furthermore, analyzing interaction networks of the genes from the ELA-associated modules and the SAD-related MAPK3 revealed complex interactions of those genes. Gene functional enrichment analyses indicate a role of signal transduction pathways as well as inflammatory responses supporting an involvement of the immune system in the association of ELA and SAD. In conclusion, we did not identify a direct molecular link between ELA and adult SAD by transcriptional changes. However, our data indicate an indirect association of ELA and SAD mediated by the interaction of genes involved in immune-related signal transduction.
Migrant mothers’ experiences of Caesarean section: a transcultural qualitative study
Authors: Rodriguez Juliette , Moro Marie Rose , Radjack Rahmeth
Published: April 2023

Objectives

Migrant women face an increased risk of adverse obstetrical outcome and Caesarean section. The psychological experience of Caesarean section combines physiological, social, and cultural dimensions. This qualitative study explores the subjective experiences of first-generation migrant women who gave birth by Caesarean section.

Methods

Seven qualitative, semi-directed interviews were conducted from January to March 2022 in a Paris maternity hospital, with women in their postpartum period who had had a scheduled or emergency Caesarean section and straightforward obstetrical outcomes. The presence of an interpreter-mediator was systematically offered. Thematic analysis of the interviews was carried out following the Interpretative Phenomenological Analysis (IPA) methodology.

Results

Four themes were identified in the thematic analysis relating to the women’s experiences of Caesarean section: (1) The shock of the intervention combines disappointment, fear and early separation from the baby, (2) Pregnancy and delivery far from one’s family aggravates the psychological suffering caused by isolation and loneliness related to migration, (3) The lack of cultural representations of Caesarean section leads to negative preconceptions and hinders mental preparation, in contrast with traditional or medicalised childbirth, and (4) The women’s experiences of the medical follow-up highlights the importance of the continuity of care.

Discussion and conclusion

Caesarean section, which is a physical break, re-enacts the symbolic break (cultural, social, familial) that follows on from emigration. Improvements in care include the need for a better preparation for Caesarean section, active efforts for care continuity, and the development of early prevention interviews and groups in maternity units.
A cost analysis of robotic vs. video-assisted thoracic surgery: The impact of the learning curve and the COVID-19 pandemic
Authors: Harrison Oliver J. , Maraschi Alessandro , Routledge Tom , Lampridis Savvas , LeReun Corinne , Bille Andrea
Published: April 2023

Introduction

Robot-assisted thoracoscopic surgery (RATS) is an alternative to video-assessed thoracoscopic surgery (VATS) for the treatment of lung cancer but concern exists regarding the high associated costs. The COVID-19 pandemic added further financial pressure to healthcare systems. This study investigated the impact of the learning curve on the cost-effectiveness of RATS lung resection and the financial impact of the COVID-19 pandemic on a RATS program.

Methods

Patients undergoing RATS lung resection between January 2017 and December 2020 were prospectively followed. A matched cohort of VATS cases were analyzed in parallel. The first 100 and most recent 100 RATS cases performed at our institution were compared to assess the learning curve. Cases performed before and after March 2020 were compared to assess the impact of the COVID-19 pandemic. A comprehensive cost analysis of multiple theatre and postoperative data points was performed using Stata statistics package (v14.2).

Results

365 RATS cases were included. Median cost per procedure was £7,167 and theatre cost accounted for 70%. Major contributing factors to overall cost were operative time and postoperative length of stay. Cost per case was £640 less after passing the learning curve (p < 0.001) largely due to reduced operative time. Comparison of a post-learning curve RATS subgroup matched to 101 VATS cases revealed no significant difference in theatre costs between the two techniques. Overall cost of RATS lung resections performed before and during the COVID-19 pandemic were not significantly different. However, theatre costs were significantly cheaper (£620/case; p < 0.001) and postoperative costs were significantly more expensive (£1,221/case; p = 0.018) during the pandemic.

Discussion

Passing the learning curve is associated with a significant reduction in the theatre costs associated with RATS lung resection and is comparable with the cost of VATS. This study may underestimate the true cost benefit of passing the learning curve due to the effect of the COVID-19 pandemic on theatre costs. The COVID-19 pandemic made RATS lung resection more expensive due to prolonged hospital stay and increased readmission rate. The present study offers some evidence that the initial increased costs associated with RATS lung resection may be gradually offset as a program progresses.