bluuebunny/crossref_metadata_2025 huggingface dataset:
Moesin (MSN) as a Novel Proteome-Based Diagnostic Marker for Early Detection of Invasive Bladder Urothelial Carcinoma in Liquid-Based Cytology
Authors: Park Jeong Hwan , Lee Cheol , Han Dohyun , Lee Jae Seok , Lee Kyung Min , Song Min Ji , Kim Kwangsoo , Lee Heonyi , Moon Kyung Chul , Kim Youngsoo , Jung Minsun , Moon Ji Hye , Lee Hyebin , Ryu Han Suk
Published: April 2020

Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.
Current Strategies and Future Perspectives for Precision Medicine in Pancreatic Cancer
Authors: Regel Ivonne , Mayerle Julia , Ujjwal Mukund Mahajan
Published: April 2020

Current standard-of-care for patients with pancreatic ductal adenocarcinoma (PDAC) focusses on chemotherapeutic regimens and pancreatic cancer surgery. However, limited treatment options, late diagnosis in advanced tumor stages and the aggressive behavior of PDAC contribute to the high mortality of the disease. Consequently, there is an urgent need of precision medicine for pancreatic cancer patients. All over the world, numerous initiatives started in recent years to translate novel scientific discoveries into prospective clinical trials. One major approach pursues the stratification of PDAC patients according the tumor transcriptome to predict treatment response. Other strategies concentrate on genomic alterations and the identification of individualized targeted therapies. Further experimental studies are ongoing to detect novel biomarkers for cancer diagnosis, subtyping, treatment response prediction or clinical outcome. However, the challenge remains to transfer the knowledge into clinical practice. In this review, we summarize current literature and knowledge and highlight novel concepts of basic and clinical research uncovering suitable biomarkers and targeted therapies. Thus, we provide an overview of preclinical and clinical efforts of precision medicine in pancreatic cancer.
CeO2 for Water Remediation: Comparison of Various Advanced Oxidation Processes
Authors: Fiorenza Roberto , Balsamo Stefano Andrea , D’Urso Luisa , Sciré Salvatore , Brundo Maria Violetta , Pecoraro Roberta , Scalisi Elena Maria , Privitera Vittorio , Impellizzeri Giuliana
Published: April 2020

Three different Advanced Oxidation Processes (AOPs) have been investigated for the degradation of the imidacloprid pesticide in water: photocatalysis, Fenton and photo-Fenton reactions. For these tests, we have compared the performance of two types of CeO2, employed as a non-conventional photocatalyst/Fenton-like material. The first one has been prepared by chemical precipitation with KOH, while the second one has been obtained by exposing the as-synthetized CeO2 to solar irradiation in H2 stream. This latter treatment led to obtain a more defective CeO2 (coded as “grey CeO2”) with the formation of Ce3+ sites on the surface of CeO2, as determined by Raman and X-ray Photoelectron Spectroscopy (XPS) characterizations. This peculiar feature has been demonstrated as beneficial for the solar photo–Fenton reaction, with the best performance exhibited by the grey CeO2. On the contrary, the bare CeO2 showed a photocatalytic activity higher with respect to the grey CeO2, due to the higher exposed surface area and the lower band-gap. The easy synthetic procedures of CeO2 reported here, allows to tune and modify the physico-chemical properties of CeO2, allowing a choice of different CeO2 samples on the basis of the specific AOPs for water remediation. Furthermore, neither of the samples have shown any critical toxicity.
E2F1 Regulates Adipocyte Differentiation and Adipogenesis by Activating ICAT
Authors: Chen Jingqing , Yang Yuchen , Li Shuai , Yang Ying , Dai Zhaolai , Wang Fengchao , Wu Zhenlong , Tso Patrick , Wu Guoyao
Published: April 2020

Wnt/β-catenin is a crucial repressor of adipogenesis. We have shown that E2 promoter binding factor 1 (E2F1) suppresses Wnt/β-catenin activity through transactivation of β-catenin interacting protein 1 (CTNNBIP1), also known as inhibitor of β-catenin and TCF4 (ICAT) in human colorectal cancers. However, it remains unknown whether ICAT is required for E2F1 to promote differentiation by inhibiting β-catenin activity in pre-adipocytes. In the present study, we found that 1-methyl-3-isobutylxanthine, dexamethasone, and insulin (MDI)-induced differentiation and lipid accumulation in 3T3-L1 pre-adipocytes was reversed by activation of β-catenin triggered by CHIR99021, a GSK3β inhibitor. Intriguingly, we observed a reduced protein level of E2F1 and ICAT at a later stage of pre-adipocytes differentiation. Importantly, overexpression of ICAT in 3T3-L1 pre-adipocytes markedly promote the adipogenesis and partially reversed the inhibitory effect of CHIR99021 on MDI-induced adipogenesis and lipid accumulation by regulating adipogenic regulators and Wnt/β-catenin targets. Moreover, pre-adipocytes differentiation induced by MDI were markedly inhibited in siE2F1 or siICAT transfected 3T3-L1 cells. Gene silencing of ICAT in the E2F1 overexpressed adipocytes also inhibited the adipogenesis. These data indicated that E2F1 is a metabolic regulator with an ability to promote pre-adipocyte differentiation by activating ICAT, therefore represses Wnt/β-catenin activity in 3T3-L1 cells. We also demonstrated that ICAT overexpression did not affect oleic acid-induced lipid accumulation at the surface of Hela and HepG2 cells. In conclusion, we show that E2F1 is a critical regulator with an ability to promote differentiation and adipogenesis by activating ICAT in pre-adipocytes.
Metabolome Profiling Supports the Key Role of the Spike in Wheat Yield Performance
Authors: Vergara-Diaz Omar , Vatter Thomas , Vicente Rubén , Obata Toshihiro , Nieto-Taladriz Maria Teresa , Aparicio Nieves , Carlisle Kefauver Shawn , Fernie Alisdair , Araus José Luis
Published: April 2020

Although the relevance of spike bracts in stress acclimation and contribution to wheat yield was recently revealed, the metabolome of this organ and its response to water stress is still unknown. The metabolite profiles of flag leaves, glumes and lemmas were characterized under contrasting field water regimes in five durum wheat cultivars. Water conditions during growth were characterized through spectral vegetation indices, canopy temperature and isotope composition. Spike bracts exhibited better coordination of carbon and nitrogen metabolisms than the flag leaves in terms of photorespiration, nitrogen assimilation and respiration paths. This coordination facilitated an accumulation of organic and amino acids in spike bracts, especially under water stress. The metabolomic response to water stress also involved an accumulation of antioxidant and drought tolerance related sugars, particularly in the spikes. Furthermore, certain cell wall, respiratory and protective metabolites were associated with genotypic outperformance and yield stability. In addition, grain yield was strongly predicted by leaf and spike bracts metabolomes independently. This study supports the role of the spike as a key organ during wheat grain filling, particularly under stress conditions and provides relevant information to explore new ways to improve wheat productivity including potential biomarkers for yield prediction.
Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation
Authors: Saveri Paola , De Luca Maria , Nisi Veronica , Pisciotta Chiara , Romano Roberta , Piscosquito Giuseppe , Reilly Mary M. , Polke James M. , Cavallaro Tiziana , Fabrizi Gian Maria , Fossa Paola , Cichero Elena , Lombardi Raffaella , Lauria Giuseppe , Magri Stefania , Taroni Franco , Pareyson Davide , Bucci Cecilia
Published: April 2020

The rare autosomal dominant Charcot-Marie-Tooth type 2B (CMT2B) is associated with mutations in the RAB7A gene, involved in the late endocytic pathway. CMT2B is characterized by predominant sensory loss, ulceromutilating features, with lesser-to-absent motor deficits. We characterized clinically and genetically a family harboring a novel pathogenic RAB7A variant and performed structural and functional analysis of the mutant protein. A 39-year-old woman presented with early-onset walking difficulties, progressive distal muscle wasting and weakness in lower limbs and only mild sensory signs. Electrophysiology demonstrated an axonal sensorimotor neuropathy. Nerve biopsy showed a chronic axonal neuropathy with moderate loss of all caliber myelinated fibers. Next-generation sequencing (NGS) technology revealed in the proband and in her similarly affected father the novel c.377A>G (p.K126R) heterozygous variant predicted to be deleterious. The mutation affects the biochemical properties of RAB7 GTPase, causes altered interaction with peripherin, and inhibition of neurite outgrowth, as for previously reported CMT2B mutants. However, it also shows differences, particularly in the epidermal growth factor receptor degradation process. Altogether, our findings indicate that this RAB7A variant is pathogenic and widens the phenotypic spectrum of CMT2B to include predominantly motor CMT2. Alteration of the receptor degradation process might explain the different clinical presentations in this family.
Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair
Authors: Bendix Poul Martin , Simonsen Adam Cohen , Florentsen Christoffer D. , Häger Swantje Christin , Mularski Anna , Zanjani Ali Asghar Hakami , Moreno-Pescador Guillermo , Klenow Martin Berg , Sønder Stine Lauritzen , Danielsen Helena M. , Arastoo Mohammad Reza , Heitmann Anne Sofie , Pandey Mayank Prakash , Lund Frederik Wendelboe , Dias Catarina , Khandelia Himanshu , Nylandsted Jesper
Published: April 2020

The plasma membrane surrounds every single cell and essentially shapes cell life by separating the interior from the external environment. Thus, maintenance of cell membrane integrity is essential to prevent death caused by disruption of the plasma membrane. To counteract plasma membrane injuries, eukaryotic cells have developed efficient repair tools that depend on Ca2+- and phospholipid-binding annexin proteins. Upon membrane damage, annexin family members are activated by a Ca2+ influx, enabling them to quickly bind at the damaged membrane and facilitate wound healing. Our recent studies, based on interdisciplinary research synergy across molecular cell biology, experimental membrane physics, and computational simulations show that annexins have additional biophysical functions in the repair response besides enabling membrane fusion. Annexins possess different membrane-shaping properties, allowing for a tailored response that involves rapid bending, constriction, and fusion of membrane edges for resealing. Moreover, some annexins have high affinity for highly curved membranes that appear at free edges near rupture sites, a property that might accelerate their recruitment for rapid repair. Here, we discuss the mechanisms of annexin-mediated membrane shaping and curvature sensing in the light of our interdisciplinary approach to study plasma membrane repair.
Natural and Engineered Electron Transfer of Nitrogenase
Authors: Gu Wenyu , Milton Ross D.
Published: April 2020

As the only enzyme currently known to reduce dinitrogen (N2) to ammonia (NH3), nitrogenase is of significant interest for bio-inspired catalyst design and for new biotechnologies aiming to produce NH3 from N2. In order to reduce N2, nitrogenase must also hydrolyze at least 16 equivalents of adenosine triphosphate (MgATP), representing the consumption of a significant quantity of energy available to biological systems. Here, we review natural and engineered electron transfer pathways to nitrogenase, including strategies to redirect or redistribute electron flow in vivo towards NH3 production. Further, we also review strategies to artificially reduce nitrogenase in vitro, where MgATP hydrolysis is necessary for turnover, in addition to strategies that are capable of bypassing the requirement of MgATP hydrolysis to achieve MgATP-independent N2 reduction.
Translational-Invariant Bipolarons and Superconductivity
Authors: Lakhno Victor D.
Published: April 2020

A translation-invariant (TI) bipolaron theory of superconductivity based, like Bardeen–Cooper–Schrieffer theory, on Fröhlich Hamiltonian is presented. Here the role of Cooper pairs belongs to TI bipolarons which are pairs of spatially delocalized electrons whose correlation length of a coupled state is small. The presence of Fermi surface leads to the stabilization of such states in its vicinity and a possibility of their Bose–Einstein condensation (BEC). The theory provides a natural explanation of the existence of a pseudogap phase preceding the superconductivity and enables one to estimate the temperature of a transition T * from a normal state to a pseudogap one. It is shown that the temperature of BEC of TI bipolarons determines the temperature of a superconducting transition T c which depends not on the bipolaron effective mass but on the ordinary mass of a band electron. This removes restrictions on the upper limit of T c for a strong electron-phonon interaction. A natural explanation is provided for the angular dependence of the superconducting gap which is determined by the angular dependence of the phonon spectrum. It is demonstrated that a lot of experiments on thermodynamic and transport characteristics, Josephson tunneling and angle-resolved photoemission spectroscopy (ARPES) of high-temperature superconductors does not contradict the concept of a TI bipolaron mechanism of superconductivity in these materials. Possible ways of enhancing T c and producing new room-temperature superconductors are discussed on the basis of the theory suggested.
Fast Phase-Contrast Cine MRI for Assessing Intracranial Hemodynamics and Cerebrospinal Fluid Dynamics
Authors: Ohno Naoki , Miyati Tosiaki , Noda Tomohiro , Alperin Noam , Hamaguchi Takashi , Ohno Masako , Matsushita Tatsuhiko , Mase Mitsuhito , Gabata Toshifumi , Kobayashi Satoshi
Published: April 2020

We propose fast phase-contrast cine magnetic resonance imaging (PC-cine MRI) to allow breath-hold acquisition, and we compared intracranial hemo- and hydrodynamic parameters obtained during breath holding between full inspiration and end expiration. On a 3.0 T MRI, using electrocardiogram (ECG)-synchronized fast PC-cine MRI with parallel imaging, rectangular field of view, and segmented k-space, we obtained velocity-mapped phase images at the mid-C2 level with different velocity encoding for transcranial blood flow and cerebrospinal-fluid (CSF) flow. Next, we calculated the peak-to-peak amplitudes of cerebral blood flow (ΔCBF), cerebral venous outflow, intracranial volume change, CSF pressure gradient (ΔPG), and intracranial compliance index. These parameters were compared between the proposed and conventional methods. Moreover, we compared these parameters between different utilized breath-hold maneuvers (inspiration, expiration, and free breathing). All parameters derived from the fast PC method agreed with those from the conventional method. The ΔPG was significantly higher during full inspiration breath holding than at the end of expiration and during free breathing. The proposed fast PC-cine MRI reduced scan time (within 30 s) with good agreement with conventional methods. The use of this method also makes it possible to assess the effects of respiration on intracranial hemo- and hydrodynamics.
Correction: Martínez-Cagigal, V.; Santamaría-Vázquez, E.; Hornero, R. Asynchronous Control of P300-Based Brain–Computer Interfaces Using Sample Entropy. Entropy 2019, 21, 230
Authors: Martínez-Cagigal Víctor , Santamaría-Vázquez Eduardo , Hornero Roberto
Published: April 2020

Figure 5 of the original paper contains errors [...]
Torrefaction of Straw from Oats and Maize for Use as a Fuel and Additive to Organic Fertilizers—TGA Analysis, Kinetics as Products for Agricultural Purposes
Authors: Szufa Szymon , Wielgosiński Grzegorz , Piersa Piotr , Czerwińska Justyna , Dzikuć Maria , Adrian Łukasz , Lewandowska Wiktoria , Marczak Marta
Published: April 2020

This publication presents research work which contains the optimum parameters of the agri-biomass: maize and oat straws torrefaction process. Parameters which are the most important for the torrefaction process and its products are temperature and residence time. Thermogravimetric analysis was performed as well as the torrefaction process using an electrical furnace on a laboratory scale at a temperature between 250–525 °C. These biomass torrefaction process parameters—residence time and temperature—were necessary to perform the design and construction of semi-pilot scale biomass torrefaction installations with a regimental dryer and a woody and agri-biomass regimental torrefaction reactor to perform a continuous torrefaction process using superheated steam. In the design installation the authors also focused on biochar, a bi-product of biofuel which will be used as an additive for natural bio-fertilizers. Kinetic analysis of torrefaction process using maize and oat straws was performed using NETZSCH Neo Kinetics software. It was found that kinetic analysis methods conducted with multiple heating rate experiments were much more efficient than the use of a single heating rate. The best representations of the experimental data for the straw from maize straw were found for the n-order reaction model. A thermogravimetric analysis, TG-MS analysis and VOC analysis combined with electrical furnace installation were performed on the maize and oat straw torrefaction process. The new approach in the work presented is different from that of current scientific achievements due to the fact that until now researchers have worked on performing processes on oat and maize straws by means of the torrefaction process for the production of a biochar as an additive for natural bio-fertilizers. None of them looked for economically reasonable mass loss ratios. In this work the authors made the assumption that a mass loss in the area of 45–50% is the most reasonable loss for the two mentioned agri-biomass processes. On this basis, a semi-pilot installation could be produced in a further BIOCARBON project step. The kinetic parameters which were calculated will be used to estimate the size of the apparatuses, the biomass dryer, and biomass torrefaction reactor.
PI Parameter Influence on Underfloor Heating Energy Consumption and Setpoint Tracking in nZEBs
Authors: Kull Tuule Mall , Thalfeldt Martin , Kurnitski Jarek
Published: April 2020

In rooms with underfloor heating (UFH), local on–off controllers most often regulate the air temperature with poor accuracy and energy penalties. It is known that proportional–integral (PI) controllers can regulate most processes more precisely. However, hydronic UFH systems have long time constants, especially in low-energy buildings, and PI parameters are not easy to set manually. In this work, several potential PI parameter estimation methods were applied, including optimizing the parameters in GenOpt, calculating the parameters based on simplified models, and tuning the parameters automatically in Matlab. For all found parameter combinations, the energy consumption and control precision were evaluated. Simpler methods were compared to the optimal solutions to find similar parameters. Compared with an on–off controller with a 0.5 K dead-band, the best PI parameter combination found was with a proportional gain of 18 and an integration time of 2300 s, which could decrease the energy consumption for heating by 9% and by 5% compared with default PI parameters. Moreover, while GenOpt was the best method to find the optimal parameters, it was also possible with a simple automatic test and calculation within a weekend. The test can be, for example, 6-h setbacks applied during the nights or weekend-long pseudo-random changes in the setpoint signal. The parameters can be calculated based on the simplified model from these tests using any well-known simple method. Results revealed that the UFH PI controller with the correct parameters started to work in a predictive fashion and the resulting room temperature curves were practically ideal.
Assessment of Agro-Environmental Impacts for Supplemented Methods to Biochar Manure Pellets during Rice (Oryza sativa L.) Cultivation
Authors: Shin JoungDu , Park SangWon , Jeong Changyoon
Published: April 2020

The agro-environmental impact of supplemented biochar manure pellet fertilizer (SBMPF) application was evaluated by exploring changes of the chemical properties of paddy water and soil, carbon sequestration, and grain yield during rice cultivation. The treatments consisted of (1) the control (no biochar), (2) pig manure compost pellet (PMCP), (3) biochar manure pellets (BMP) with urea solution heated at 60 °C (BMP-U60), (4) BMP with N, P, and K solutions at room temperature (BMP-NPK), and (5) BMP with urea and K solutions at room temperature (BMP-UK). The NO3−–N and PO4−–P concentrations in the control and PMCP in the paddy water were relatively higher compared to SBMPF applied plots. For paddy soil, NH4+–N concentration in the control was lower compared to the other SBMPFs treatments 41 days after rice transplant. Additionally, it is possible that the SBMPFs could decrease the phosphorus levels in agricultural ecosystems. Also, the highest carbon sequestration was 2.67 tonnes C ha−1 in the BMP-UK treatment, while the lowest was 1.14 tonnes C ha−1 in the BMP-U60 treatment. The grain yields from the SBMPFs treatments except for the BMP-UK were significantly higher than the control. Overall, it appeared that the supplemented BMP-NPK application was one of the best SBMPFs considered with respect to agro-environmental impacts during rice cultivation.
Hot Water Extraction: Short Rotation Willow, Mixed Hardwoods, and Process Considerations
Authors: Wood Christopher D. , Amidon Thomas E. , Volk Timothy A. , Emerson Rachel M.
Published: April 2020

Short rotation woody crops (SRWC) like shrub willow are highly productive biomass resources of interest for energy and fuel applications. Hot water extraction (HWE) as an upgrading tool to enable the use of willow biomass in pellet applications has been proposed, and is of increasing interest. This study treats willow and mixes of willow and conventional mixed hardwood feedstock with HWE in a tumbling laboratory reactor to elucidate the effects of time, temperature, feedstock mixes, and other process considerations (water:biomass ratio, presteaming, counter-current processing) on mass removals and other extraction outcomes (e.g., sugar, acetate, and furan yields). Results demonstrated alignment of extraction outcomes with P-factor from 155 °C to 175 °C, with a good compromise of removed mass and co-product potential in the range from 575–800 P-factor. The preferred condition was chosen as 575 P-factor. HWE of mixes of willow and hardwood feedstocks showed a linear response of extraction outcomes to willow:hardwood ratios. Testing of water:biomass ratios demonstrated that this is a significant consideration, with each outcome being affected somewhat differently, and indicating that HWE is more diffusion dependent than expected. Presteaming shows little to no effect on extraction outcomes, while multi-stage cooks simulating counter-current operation indicate a significant potential value in counter-current extraction.
Minimizing Power Consumption of an Experimental HVAC System Based on Parallel Grid Searching
Authors: Xiong Wangqi , Wang Jiandong
Published: April 2020

This paper proposes a parallel grid search algorithm to find an optimal operating point for minimizing the power consumption of an experimental heating, ventilating and air conditioning (HVAC) system. First, a multidimensional, nonlinear and non-convex optimization problem subject to constraints is formulated based on a semi-physical model of the experimental HVAC system. Second, the optimization problem is parallelized based on Graphics Processing Units to simultaneously compute optimization loss functions for different solutions in a searching grid, and to find the optimal solution as the one having the minimum loss function. The proposed algorithm has an advantage that the optimal solution is known with evidence as to the best one subject to current resolutions of the searching grid. Experimental studies are provided to support the proposed algorithm.
Genetic Characterization of Cupped Oyster Resources in Europe Using Informative Single Nucleotide Polymorphism (SNP) Panels
Authors: Lapègue Sylvie , Heurtebise Serge , Cornette Florence , Guichoux Erwan , Gagnaire Pierre-Alexandre
Published: April 2020

The Pacific oyster, Crassostrea gigas, was voluntarily introduced from Japan and British Columbia into Europe in the early 1970s, mainly to replace the Portuguese oyster, Crassostrea angulata, in the French shellfish industry, following a severe disease outbreak. Since then, the two species have been in contact in southern Europe and, therefore, have the potential to exchange genes. Recent evolutionary genomic works have provided empirical evidence that C. gigas and C. angulata exhibit partial reproductive isolation. Although hybridization occurs in nature, the rate of interspecific gene flow varies across the genome, resulting in highly heterogeneous genome divergence. Taking this biological property into account is important to characterize genetic ancestry and population structure in oysters. Here, we identified a subset of ancestry-informative makers from the most differentiated regions of the genome using existing genomic resources. We developed two different panels in order to (i) easily differentiate C. gigas and C. angulata, and (ii) describe the genetic diversity and structure of the cupped oyster with a particular focus on French Atlantic populations. Our results confirm high genetic homogeneity among Pacific cupped oyster populations in France and reveal several cases of introgressions between Portuguese and Japanese oysters in France and Portugal.
First Molecular Diagnosis of Clinical Cases of Gastric Anisakiosis in Spain
Authors: Roca-Geronès Xavier , Alcover M. Magdalena , Godínez-González Carla , González-Moreno Olga , Masachs Miquel , Fisa Roser , Montoliu Isabel
Published: April 2020

Anisakiosis is a fish-borne disease with gastrointestinal and/or allergic symptoms caused by the consumption of raw or undercooked fish parasitized with nematode larvae of the genus Anisakis. In Europe, Anisakis pegreffii has been detected as the causative agent, although the sibling species Anisakis simplex sensu stricto (s.s.) is also known to cause the disease in other parts of the world, and discrepancies exist regarding their respective pathogenic potential. In Spain a high number of cases has been recorded, with marinated anchovies being the main source of infection, although no specific diagnosis has been documented in humans. In this study, we analyzed three cases of anisakiosis in patients from Barcelona (Spain) who had consumed undercooked hake. All patients described epigastric pain and several larval nematodes were removed endoscopically from their stomachs. Larvae were morphologically characterized as third-stage larvae of Anisakis simplex sensu lato (s.l.) and molecularly identified as A. simplex (s.s.) by means of PCR RFLP of the ITS region of the rDNA and sequencing of the elongation factor1 alpha1 (EF1 α-1) nDNA gen. This study represents the first specific identification of Anisakis larvae in clinical cases of anisakiosis reported in Spain. Specific molecular diagnosis is of crucial importance for assessing the health risk of Anisakis sibling species. Hake consumption stands out as a risk factor for anisakiosis, since this fish species can be highly parasitized.
Research on Evaluation Indexes and Weights of the Aging-Friendly Community Public Environment under the Community Home-based Pension Model
Authors: Mei Wen-Bing , Hsu Che-Yu , Ou Sheng-Jung
Published: April 2020

Community home-based care has become China’s main mode of care for the elderly, and the aging of the community public environment has become the focus of attention of all of society. This study uses a questionnaire survey and the fuzzy analytic hierarchy process (FAHP) to (i) obtain the relative weights of indicators in the hierarchy structure of an aging-friendly community public environment and (ii) build a complete indicator evaluation system for the aging-friendly community public environment. The research results show that the quasi-side evaluation index framework of the aging-friendly community public environment is composed of four factors (i.e., community facilities, community road system, community environmental function, and community landscape configuration) and 24 evaluation indexes. The weights of the indicators in descending order are “community road system (w = 0.374)”, “community facilities (w = 0.310)”, “community environmental functions (w = 0.264)”, and “community landscape configuration (w = 0.052)”. The research results show that "community road systems” and “community facilities” are important indicators of the aging-friendliness of a community public environment. “Community environmental function” is an important supplemental factor of the aging-friendliness of a community public environment. “Community landscape configuration” involves improving the construction of the community public environment from the perspective of landscaping. Among all indicator levels, the weights of “Community road floor slip resistance” (w = 0.1795), “Daily health and medical facilities (w = 0.1181)”, and “Provide social interaction functions (w = 0.1067)” are ranked the highest. These results show that ensuring the physical and mental health of the elderly in the community is a core criterion for evaluating the aging-friendliness of a public environment in the community. In this study, an index evaluation weight system is established to clarify the best approach to constructing an aging-friendly community public environment in accordance with previous standard specifications. This system can further clarify the scientific method for evaluating aging-friendly public environments built in the past and can serve as a reference for the practical world.
“Living Well” in the Constitution of Bolivia and the American Declaration on the Rights of Indigenous Peoples: Reflections on Well-Being and the Right to Development
Authors: Añaños Bedriñana Karen Giovanna , Hernández Umaña Bernardo Alfredo , Rodríguez Martín José Antonio
Published: April 2020

The article analyzes how approaches to “Living Well” as reflected in the Constitution of the State of Bolivia, the Law of the Rights of Mother Earth, and the American Declaration on the Rights of Indigenous Peoples of the Organization of American States (OAS) contribute to understanding the Andean cosmovision of indigenous peoples of the American continent. To do so, it first studied the most immediate precedents that led to incorporation of the notion of Living Well into Bolivian law. Second, it approached the right to development from the American Declaration on the Rights of Indigenous Peoples, which has as its source the United Nations Declaration of the Rights of Indigenous Peoples. The paper thus proposes reflections on the Bolivian State and the American Declaration that advance understanding of Living Well, a notion comparable in the West to the right to development (political, social, economic, environmental, and cultural) that enables the individual and collective realization of the individual. Fullness, understood in terms of well-being, is related to the protection of health and of the environment. Finally, the paper employs a qualitative methodology with a well-documented hermeneutic focus, as well as the tool of a semi-structured interview with a Bolivian scholar familiar on the topic.
What Keeps Them Physically Active? Predicting Physical Activity, Motor Competence, Health-Related Fitness, and Perceived Competence in Irish Adolescents after the Transition from Primary to Second-Level School
Authors: Britton Una , Issartel Johann , Symonds Jennifer , Belton Sarahjane
Published: April 2020

Physical activity (PA) decreases with age. The school transition is noted for significant changes in PA behaviour. Motor competence (MC), health-related fitness (HRF), and perceived competence (PC) are generally positively associated with PA. The aim of this study was to examine longitudinal cross-lagged relationships between PA, MC, HRF, and PC across the school transition from final year of primary school to first year of second-level school in Irish youth. PA (accelerometery), object-control and locomotor MC (TGMD-III), PC (perceived athletic competence subscale of the Self-Perception Profile for Adolescents), and HRF (20 m shuttle run, horizontal jump, vertical jump, push-ups, curl-ups) were measured in final year of primary school (6th class) and first year of second-level school (1st year). In the sixth class, 261 participants (53% female; mean age 12.22 ± 0.48 years) were tested. In first year, 291 participants (48% female; mean age: 13.20 ± 0.39 years) were tested. In total, 220 participants were involved in the study at both timepoints. Cross-lagged regression in AMOS23, using full information maximum likelihood estimation, was conducted to test reciprocal and predictive pathways between variables. The full cross-lagged model showed acceptable fit (χ2 = 69.12, df = 8, p < 0.01, NFI = 0.93, CFI = 0.94). HRF was the strongest predictor of future PA (β = 0.353), and also predicted PC (β = 0.336) and MC (β = 0.163). Object-control MC predicted future PA (β = 0.192). Reciprocal relationships existed between object-control MC and PA, and between object-control MC and PC. HRF was the strongest predictor of PA. Object-control MC also predicted PA. PA promotion strategies should target the development of HRF and object-control MC in primary school to reduce the decline in PA frequently observed after the school transition.
The Association of Work Satisfaction and Burnout Risk in Endoscopy Nursing Staff—A Cross-Sectional Study Using Canonical Correlation Analysis
Authors: Adarkwah Charles Christian , Hirsch Oliver
Published: April 2020

Background: Burnout is known to have detrimental effects on healthcare staff with regard to both personal and occupational matters. The association between burnout symptoms and work satisfaction in endoscopy nursing staff in Germany has not been studied previously. We aimed to investigate the association between work satisfaction and risk of burnout in endoscopy nursing staff in Germany and to extract predictors for burnout in the area of work satisfaction, which can inform the design of future interventions. Setting: All members of the German Association of Endoscopy Staff in Germany (Deutsche Gesellschaft für Endoskopiefachberufe e.V.—DEGEA) were invited to take part in an online survey. Methods: The total sample consisted of 674 endoscopy staff members. Of those, 579 were female (85.9%) and 95 were male (14.1%). The mean age of the participants was 44.3 years (SD 10.6), with a median age of 46 years, a minimum age of 20, and a maximum age of 64 years. We used confirmatory factor analyses to examine the Maslach burnout inventory (MBI) and, a questionnaire for assessing general and facet-specific job satisfaction (KAFA), regarding their postulated internal structure in our special sample. Canonical correlations were performed to examine the association between work satisfaction and burnout in endoscopy staff members. Results: We were able to replicate the factorial structures of the MBI and the KAFA, both showing an acceptable model fit. The canonical correlation analysis resulted in three canonical functions, with canonical correlations of 0.64 (p < 0.001), 0.32 (p < 0.001), and 0.17 (p < 0.001). The first canonical function revealed that KAFA scales for colleagues, professional development, payment, supervisor, and general job satisfaction were good predictors for less exhaustion, less depersonalization and lack of empathy, and higher personal accomplishment. Commonality analysis revealed that general job satisfaction was the most significant factor in explaining the squared canonical correlation. The second canonical function showed that occupational function and colleagues were good predictors for exhaustion and personal accomplishment. Conclusions: Interventions aimed at ameliorating symptoms of burnout in endoscopy staff should be tailored to address specific needs as experienced by the employees. Therefore, the results of this study could contribute to the design of various interventions, which could be employed to address the issue of work satisfaction and burnout in endoscopy staff most effectively.
Can the Psychosocial Safety Climate Reduce Ill-Health Presenteeism? Evidence from Chinese Healthcare Staff under a Dual Information Processing Path Lens
Authors: Liu Beini , Lu Qiang , Zhao Yue , Zhan Jing
Published: April 2020

Because of heavy workloads, non-transferable responsibilities, and shift systems, healthcare staff are prone to ill-health presenteeism. Based on social information processing theory, this study explored the influence of the psychosocial safety climate (PSC) on ill-health presenteeism. The mediating effects of perceived instrumental support and perceived emotional support and the moderating effect of organic structure in this process were observed. Using a time-lagged research design, data from 386 healthcare staff were gathered and multiple regression and bootstrapping were used to test each hypothesis. The results showed that: (1) PSC negatively relates to ill-health presenteeism. (2) Both perceived instrumental support and perceived emotional support mediate the relationship between PSC and ill-health presenteeism. The affective information processing path is more effective than the cognitive information processing path, but they do not convey a positive interaction effect on ill-health presenteeism. (3) The organic structure moderates the mediating effect of perceived emotional support but does not exert a significant moderating effect on the mediating process of perceived instrumental support. This study particularly identified PSC as a contextual antecedent of ill-health presenteeism. By combining organizational, work-related, and person-related factors, a more comprehensive theoretical framework for the understanding of ill-health presenteeism is developed, thus informing health promotion management.
Skinfold Thickness Distribution in Recreational Marathon Runners
Authors: Nikolaidis Pantelis Theodoros , Rosemann Thomas , Knechtle Beat
Published: April 2020

The relationship of body fat (BF) percentage with performance of elite marathon runners has been well studied; however, less information is available about the variation of skinfold thickness by sex and performance in non-elite marathon runners. The aim of the present study was to examine the variation of skinfold thickness by sex and performance in recreational marathon runners. Participants included 32 female (age 40.1 ± 9.0 years, BF 19.6 ± 4.7%, and training volume 47.7 ± 22.6 km) and 134 male marathon runners (44.3 ± 8.8 years, 17.6 ± 4.0%, and 53.0 ± 21.2 km, respectively). The largest skinfold thickness was the abdomen in both sexes, whereas the smallest was biceps in men, and chins in women (p < 0.001). The largest sex difference in skinfold thickness was observed in triceps being the fattest in women (p < 0.001). The largest difference in skinfold thickness among men’s performance groups was observed in the iliac crest, and the smallest in the patella and proximal calf (p < 0.001). In summary, skinfold measurements indicated that women had more fat in both their upper and lower limbs, while men had more fat in their trunk. With regards to the role of performance level, the slowest runners presented relatively more fat in the upper limbs and trunk anatomical sites, i.e., away from the active muscles of legs.
CD44 Gene Polymorphisms as a Risk Factor for Susceptibility and Their Effect on the Clinicopathological Characteristics of Lung Adenocarcinoma in Male Patients
Authors: Li Ju-Pi , Hsieh Ming-Ju , Chou Ying-Erh , Chao Yu-Hua , Tsao Thomas Chang-Yao , Yang Shun-Fa
Published: April 2020

Lung adenocarcinoma is a subtype of lung cancer with high morbidity and mortality. CD44 is instrumental in many physiological and tumor pathological processes. The expression of unique single nucleotide polymorphisms (SNPs) contributes to protein dysfunction and influences cancer susceptibility. In the current study, we investigated the relationship between CD44 polymorphisms and the susceptibility to lung adenocarcinoma with or without epidermal growth factor receptor (EGFR) gene mutations. This study included 279 patients with lung adenocarcinoma. In total, six CD44 SNPs (rs1425802, rs11821102, rs10836347, rs13347, rs187115, and rs713330) were genotyped using a real-time polymerase chain reaction. We found no significant differences in genotype distribution of CD44 polymorphisms between EGFR wild-type and EGFR mutation type in patients with lung adenocarcinoma. We observed a strong association between CD44 rs11821102 G/A polymorphism and EGFR L858R mutation (odds ratio (OR) = 3.846, 95% confidence interval (CI) = 1.018–14.538; p = 0.037) compared with the EGFR wild-type group. In the subgroup of male patients with lung adenocarcinoma harboring the EGFR wild-type, both CD44 rs713330 T/C (OR = 4.317, 95% CI = 1.029–18.115; p = 0.035) and rs10836347 C/T polymorphisms (OR = 9.391, 95% CI = 1.061–83.136; p = 0.019) exhibited significant associations with tumor size and invasion. Data from the present study suggest that CD44 SNPs may help to predict cancer susceptibility and tumor growth in male patients with lung adenocarcinoma.
Urban Water Consumption Patterns in an Adult Population in Wuxi, China: A Regression Tree Analysis
Authors: Zheng Hao , Zhou Weijie , Zhang Lan , Li Xiaobo , Cheng Jian , Ding Zhen , Xu Yan , Hu Wenbiao
Published: April 2020

Understanding water intake variation is crucial for assessing human exposure to water pollutants. The correlation between water intake and demographic factors warrants further exploration. A cross-sectional study was conducted to estimate urban water consumption and its associated factors among adults in Wuxi, China, in 2015. The water consumption information was obtained by a 24-h self-report diary over seven consecutive days. A classification and regression tree (CART) analysis was applied to detect how water consumption varied with the demographic variables. Finally, a total of 1188 adults (18–87 years old) were included. The results demonstrated that the median water consumption of the adults was 1525 mL/day in summer and 1217 mL/day in winter. The results of the CART analysis demonstrated that body mass index (BMI) and age were the leading factors that were associated with water consumption in summer and winter, respectively. The water consumption threshold of BMI for men differed from women (23 kg/m2 vs. 18 kg/m2) in summer, and the threshold of age for men was also different from women (43 years vs. 21 years) in winter. In conclusion, the findings are useful for accurately assessing human exposure to water pollutants and identifying the high-risk subgroups.
Patient Accessibility and Budget Impact of Orphan Drugs in South Korea: Long-Term and Real-World Data Analysis (2007–2019)
Authors: Lee Se Hee , Yoo Seung-Lai , Bang Joon Seok , Lee Jong Hyuk
Published: April 2020

This study aimed to identify orphan drug accessibility and impact on pharmaceutical budgets in South Korea by analyzing the status of orphan drug designation, approval, reimbursement, and pharmaceutical expenditure. We analyzed the dataset on orphan drugs designated, approved, and reimbursed from 2007 to 2019 based on long-term real-world data. The designated and approved orphan drugs were 165 and 156, respectively, and 88 out of 156 approved products were reimbursed. Total expenditure on orphan drugs increased annually to account for about 1.44% of total pharmaceutical expenditure in 2018. Orphan drug expenditure per patient increased on average by 8.7% per year. The average annual cost of orphan drugs was USD 27,000–USD 47,000, with the maximum value of USD 260,000–USD 560,000. As there are a number of orphan drugs that have not yet been reimbursable after approval, a reimbursement policy should be established that considers the characteristics of orphan drugs. Since the rapid increase in orphan drug expenditure can be a potential threat to the insurance budget, budget management should also be considered. In conclusion, it is necessary to take preemptive measures to manage the health insurance budget efficiently while improving patient accessibility to orphan drugs.
Spatial Distribution of Land Surface Temperatures in Kuwait: Urban Heat and Cool Islands
Authors: Alahmad Barrak , Tomasso Linda Powers , Al-Hemoud Ali , James Peter , Koutrakis Petros
Published: April 2020

The global rise of urbanization has led to the formation of surface urban heat islands and surface urban cool islands. Urban heat islands have been shown to increase thermal discomfort, which increases heat stress and heat-related diseases. In Kuwait, a hyper-arid desert climate, most of the population lives in urban and suburban areas. In this study, we characterized the spatial distribution of land surface temperatures and investigated the presence of urban heat and cool effects in Kuwait. We used historical Moderate-Resolution Imaging Spectroradiometer (MODIS) Terra satellite 8-day composite land surface temperature (LST) from 2001 to 2017. We calculated the average LSTs of the urban/suburban governorates and compared them to the average LSTs of the rural and barren lands. We repeated the analysis for daytime and nighttime LST. During the day, the temperature difference (urban/suburban minus versus governorates) was −1.1 °C (95% CI; −1.2, −1.00, p < 0.001) indicating a daytime urban cool island. At night, the temperature difference (urban/suburban versus rural governorates) became 3.6 °C (95% CI; 3.5, 3.7, p < 0.001) indicating a nighttime urban heat island. In light of rising temperatures in Kuwait, this work can inform climate change adaptation efforts in the country including urban planning policies, but also has the potential to improve temperature exposure assessment for future population health studies.