bluuebunny/crossref_metadata_2025 huggingface dataset:
Validating field regeneration capacity for selected accessions of Gossypium hirsutum using callus induction and regeneration capacity
Authors: Tajo Sani Muhammad , Pan Zhaoe , He Shoupu , Jia Yinhua , Mahmood Tahir , Nazir Mian Fasil , Hu Daowu , Wang Liru , Sadau Salisu Bello , Ibrahim Sani , Auta Umar , Geng Xiaoli , Du Xiongming
Published: January 2023

Background: Gossypium hirsutumundergoes rapid clonal propagation to regenerate a mature plant through tissue culture. In this research, cotton leaf regeneration level for 21 accessions in the field (new leaves) was observed after the first harvest, and a comparison between field regeneration level and callus induction with its regeneration capacity (new shoots and roots) for the same 21 accessions was carried out. During the flowering stage of Gossypium hirsutum, biochemical (proline), and physiological (chlorophyll and carotenoid content) analysis was carried out. Phenotypic observations (plant height, leaf area, fresh leaf weight, dry leaf weight, number of flowers, and bolls) were also carried out on 21 accessions for each.
Result:A significant correlation between physiological parameters and callus induction was discovered. Callus induction and regeneration capacity of roots and shoots for hypocotyl, cotyledons and shoot tip tissues were used to validate field leaf regeneration level in the field after the first harvest. CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.
Conclusion:We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl, cotyledons, and shoot tip. The results of this study showed that ZS061, Lumian 378, Jimian 863, and ZS065 have the highest moisture retention capacity while CCRI 24, Liaoyangduomaomian, and Beizhegongshemian have the lowest moisture retention. CCRI 24 have the highest leaves regeneration in the field while Beizhegongshemian has lowest leaves regeneration in the field. All our result provides a clue for check the regeneration capacity through leaf regeneration level in the field.
Identification of Impacts in the Town of San Pablo de Amalí, Ecuador, by Hidrotambo S.A.’s San José del Tambo Hydroelectric Power Plant by Means of a Multitemporal Analysis of Landscape Units for the Detection of Land Use Change Over 20 Years
Authors: Marín Jorge A. Espinosa , Perez Danilo , Rivera Diego , Fuentes Rodrigo , Conrad Rchael
Published: January 2023

This study identifies the effects of land use change in the area of direct influence of the Dulcepamba River for hydroelectric production in relation to vegetation, river course, and damage to the population, among others, through multi-temporal analysis of satellite images 20- years old, this study presents conclusive results based on a methodology for detecting land use change through multitemporal analysis, which was contrasted and verified with topographic and visual field identification, in the evaluation of the 4 time periods analyzed, exists change in the river and closed areas affected with changes influence of the riverbed, which affects the area where the local population lives and carries out their economic activities. The cause-effect relationship of the anthropic interventions in the river and the impacts on the area of the "village" and the "populated area" is also evidenced by comparing the representative images of each period in which there were significant changes, from October 2001 to December 2019
Perovskite quantum dots modulating upconversion nanomaterials for cancer early detections
Authors: He Yue , Rao Hongtao , Wang JingJing , Wu Ying , Han Caiqin , Yan Changchun , Temple Hunter , Zhang Le , Chen Wei , Liu Ying
Published: January 2023

The accurate diagnosis and treatment of cancer cell lesions need a high standard of detection technology. Fluorescent probes to perform cancer biomarker detection have become a popular research issue. However, fluorescent probes still face enormous challenges of complex design and difficult detection. In this work, we propose a novel composite material UCNP@SiO2+QDs based on the combination of rare earth upconversion (UCNP) and perovskite quantum dots (QDs) and design a new fluorescent probe MB-UCNP@SiO2+QDs with molecular beacon (MB) as the carrier, that can be excited by near-infrared light, emitted in the visible wavelength, specifically identified and highly sensitive. Under the excitation of 980 nm near-infrared light, the UCNP and QDs in the composite produced the maximum efficiency of energy transfer through fluorescence resonance, and the multi-emission light of UCNP synergistically excited the re-emission of QDs, and the energy transfer efficiency is 70.6%. By changing the doping ratio of QDs halogen elements in UCNP@SiO2+QDs, it is possible to modulate the precise luminescence of UCNP@SiO2+QDs in the entire wavelength range of visible light at different positions. The novel fluorescent probe is obtained using UCNP@SiO2+QDs and Black Hole Quencher-1 (BHQ1) quenching groups linked to the two respective sides of MB, selecting as the target of detection the myeloma cancer biomarker miRNA-155, a difficult diagnostic and complex developmental type, and have achieved specific recognition and low concentration of miRNA-155 and a detection limit of 73.5 pM. This fluorescent probe design can provide new ideas for the early diagnosis and treatment of cancer, tumors, and cardiovascular diseases.
CORALIS, an R package for ncRNA-target enrichment analysis based on experimentally validated interactions
Authors: Valle-Millares Daniel , Briz Verónica , Fernández-Rodríguez Amanda
Published: January 2023

Non-coding RNAs are key regulatory molecules that govern a myriad of biological process by interacting and modulating the expression of their target protein-coding genes. The combination of RNA large-scale sequencing and computational analyses has revealed the existence of a vast diversity of RNA species with essential roles in biological functions. However, dealing with the huge network of interactions has become a challenge and the urgent need for new computational tools has arisen. We present CORALIS, an R package for the statistical analysis and visualization of experimentally validated interactions between non-coding RNAs and their target genes (ncRNA-mRNA). So far, CORALIS supports miRNA, lncRNA, snRNA and snoRNA target enrichment analysis for several species such as Homo sapiens, and other human-model species such as Rattus norvegicus, Caenorhabditis elegans and Drosophila melanogaster, among others. Statistical tests (hypergeometric distribution test) are performed to estimate enriched target genes for non-coding RNAs defined by the user, and visualization tools allows to easily analyze the results. In conclusion, CORALIS allows identifying enriched target genes given a list of non-coding RNAs. CORALIS is freely accessible at https://github.com/Daniel-VM/CORALIS.
How do multi-stakeholder partnerships influence access to quality bean seed and variety turnover? Lessons from Burundi and Zimbabwe
Authors: Aseete Paul , Katungi Enid
Published: January 2023

Background
Crop breeding is an important pathway for increasing the yield potential and one of the strategies used to adapt smallholder agriculture to climate change in developing countries. Genetic gains, however, go to waste if farmers do not access modern varieties at the right time (before seed deteriorates) and pace. Ensuring fast, prompt, and sustained access to quality bean seed, just like seed of other leguminous crops, has however been a challenge in Sub Saharan Africa. The autogamous nature of the bean crop makes sustaining seed supply and demand hard. This therefore calls for innovative approaches among bean seed value chain actors to deliver quality seed of targeted varieties. One such approach is the use of Multi-Stakeholder Partnerships (MSPs).
Methods
In this study, we assess the impacts of MSPs on seed access, quality of seed, and variety turnover measured as the Area Weighted Age of the Variety (AWAVA). These MSPs were created in Burundi and Zimbabwe to improve the performance of the bean seed system by facilitating access to quality seed. To show the average treatment effect on the treated, the study used the endogenous treatments effects estimator under the control function framework and the change in change and difference in difference framework for Zimbabwe because we had pre and post treatment data.
Results
Our findings show that the MSP was associated with significant improvement in ranges of 27 -42% more access to seed of improved bean varieties among intervention communities. Also residing in an intervention area was associated with a 65% reduction in distance to bean seed markets. Results further revealed that the MSP approach could be associated with a reduction of the AWAVA of improved varieties grown by 4.4 years among high-intensive intervention groups in Burundi and by 5.2 years for Zimbabwe. Farmers in Burundi were also generally satisfied with production, market and consumption traits of varieties supplied.
Conclusions
Our results show that using a multi partner-based seed system approach coupled with supply of quality seed of superior varieties, capacity building, and promotion efforts lead to better seed access outcomes at farm level. These findings imply that MSPs can be an effective tool for delivering new varieties of legume crops to farmers, and thus reduce varietal turnover.
The structural basis of the divalent cation blocking on tetrameric cation channel
Authors: Irie Katsumasa , Oda Yoshinori , Sumikama Takashi , Oshima Atsunori , Fujiyoshi Yoshinori
Published: January 2023

Divalent cation blocking is observed in various tetrameric ion channels. For the blocking, a divalent cation is thought to stack in the ion pathway of the channel, but this has not yet been directly observed, so the blocking mechanism by these small divalent cations remains uncertain. Here, we elucidated the divalent cation blocking mechanism by reproducing the blocking effect into NavAb, a well-studied tetrameric sodium channel. Our crystal structures of NavAb mutants showed that the mutations increasing the hydrophilicity of the inner vestibule of the pore domain enable a divalent cation to stack on the ion pathway. Furthermore, molecular dynamics simulation showed that the stacking calcium ion repels the sodium ions at the bottom of the selectivity filter. These results suggest the primary mechanism of the divalent cation block in biologically essential channels.
Delayed Neuregulin-1 Treatment Initiates a Late Neuroprotective Early Regenerative and Autophagic Spatial Profiles in Mice following Ischemic Stroke
Authors: Noll Jessica , Augello Catherine , Kurum Esra , Pan Liuliu , Pavenko Anna , Nam Andy , Ford Byron
Published: January 2023

Neuregulin-1 (NRG-1) has been shown to be neuroprotective and anti-inflammatory in rodent models following focal brain ischemia. However, the spatial transcriptional mechanisms involved in the effects of NRG-1 have not been investigated. In this study, we examined the spatial cellular and neuroinflammatory mechanisms employed by NRG-1 following stroke utilizing Nanostring Digital Spatial Profiling (DSP) technology. C57bl/6 mice were subjected to photothrombotic middle cerebral artery occlusion (MCAO). Animals were treated with NRG-1 (5ug/kg/day NRG-1 β) or vehicle beginning 24 hours after surgery, and then sacrificed at 3 days post-ischemia (dpi). Using GeoMX DSP technology, we examined the relative expression of 73 proteins in selected regions of interest (ROI) within the ischemic core, peri-infarct tissues, and peri-infarct normal tissue (PiNT) in controls and NRG-1 treated mice. FJB staining showed that delayed NRG-1 treatment did not significantly alter overall neuronal death, but it had profound effects on the neuroregenerative outgrowth environment. The ipsilateral core ROI demonstrated the most altered proteomic profile with 37 differentially regulated proteins related to autophagy and mitophagy, immune cell phagocytosis, and synaptic activity. NRG-1 upregulated synaptophysin by 2.10-fold while marginally rescuing neuronal proteins NeuN and MAP2. NRG-1 treatment induced the upregulation of ULK1, LC3B, ATG5, PINK1, and Park7 within the core region, suggesting successful autophagic flux restoration. NRG-1 treatment also increased Mertk, CD9, CSF1R/CD115, MHCII and Myelin Basic Protein (MBP). Phospho-Tau (S199) was increased 57-fold following stroke and further increased to 90-fold after NRG-1 treatment. The peri-infarct region showed increased astrocyte proteins along with BAG3, CD204/MSR1, phospho-Tau (S199) and Ki67, a marker of cell proliferation. Together, our findings showed that mechanisms of neuroprotection by NRG-1 following stroke occur in distinct spatial domains of the injured brain following ischemia. The data suggest that NRG-1 provides protection against further neuronal apoptosis and priming of an anti-inflammatory, neuroregenerative niche in a spatial manner, which could lead to novel treatment strategies for stroke.
Effect of individualized narrative nursing mode on mental health, cognitive function and limb function recovery of elderly patients with fracture complicated with cerebrovascular accident
Authors: Wang Yurong , Zhang Fang , Li Cuihua
Published: January 2023

Background: Fractures often occur in elderly patients. Osteoporosis caused by massive loss of calcium ions in the bones of elderly patients can easily lead to femoral fractures after suffering a low-and medium-energy injury. With the gradual entry of the aging society in China, the incidence of senile fracture is also gradually increasing. However, there is no report on the application of personalized narrative nursing to the mental health, cognitive function and limb function recovery of elderly patients with fracture complicated with cerebrovascular accident, in order to enhance the cognitive level of elderly patients with fracture complicated with cerebrovascular accident. This study was specially conducted with a positive attitude towards the disease and improving the life quality.
Methods: During July 2018 to July 2021, eighty elderly patients with fracture complicated with cerebrovascular accident cured were selected in our hospital. The patients were arbitrarily classified assigned control group (n=40) and study group (n=40). The former received routine nursing and the latter received personalized narrative nursing mode. The nursing satisfaction, functional independence scale (FIM), self-rating anxiety scale (SAS), self-rating depression scale (SDS), cognitive function, fracture healing time, length of hospital stays and hospitalization expenses were compared.
Results: The study group had a satisfaction rate of 100.00%, while the control group had 87.50%. The nursing satisfaction of the study group was higher (P<0.05). After 3 months of nursing, the FIM scores augmented. The FIM scores of upper and lower limbs in the study group were remarkably higher (P<0.05). A decrease in SAS and SDS scores was observed. The SAS and SDS scores of the study group were lower (P<0.05). Three months after discharge, the cognitive function score augmented. At 3 months after discharge, the study group had a higher cognitive function score (P<0.05). The fracture healing time, length of stay and cost of hospitalization in the study group were lower (P<0.05).
Conclusion: Personalized narrative nursing model can successfully enhance the mental health and cognitive function of elderly patients with fracture complicated with cerebrovascular accident, enhance the recovery of limb function, promote patients' nursing satisfaction, and alleviate the economic burden.
Effects of levamisole on haematological and oxidative stress parameters in packed donkeys; Efficacy of levamisole and ivermectin against strongyle infection in donkeys
Authors: Okaiyeto Babatunde Folayemi , Bedu Sackey Anthony Kojo , Kolo Mohammed Abdullahi , Tangang Aluwong , Salisu Ibrahim
Published: January 2023

Stress may be caused by psychological, physiological, environmental and physical factors. The aim of the study was to investigate the ameliorative and anti-oxidative effects of levamisole on stress, and the comparative efficacy of levamisole and Ivermectin in the control of Strongyle infection in donkeys. Adult male donkeys (n = 35) aged between 4–5 years; 25 naturally infested with Strongyle spp and 10 apparently healthy (free of helminth infestation) were selected for this study, divided broadly into 2 groups: Anthelmintic efficacy study group comprising 20 (10 donkeys for Ivermectin efficacy studies and the other 10 for Levamisole efficacy study on clearing strongyles from the gastro-intestinal tract of donkeys). The oxidative stress amelioration groups were A, B, C of five donkeys each: Groups A and B donkeys were the apparently healthy group; while Group C donkeys were naturally infected with Strongyle spp. All the donkeys in the oxidative stress amelioration group participated in packing of 40 kg for 10 km. Groups B and C were treated with levamisole at 7.5 mg/kg, while Group A received no treatment prior to packing. Blood was collected from all donkeys in the oxidative stress amelioration group for haemogram and oxidative stress biomarker analyses. No significant effect (P > 0.05) was observed between groups: A, B and C for: respiratory rate, pulse rate and rectal temperature; haemogram, and malondialdehyde concentration, activities of superoxide dismutase and catalase. Erythrocyte membranes were osmotically stable at 0.5% NaCl in the treated group in comparison to the controls. Packing of donkeys for 10 km did not induce significant changes in vital parameters, haemogram and biomarkers of oxidative stress, but levamisole improved erythrocyte membrane stability. It was concluded that packing for 10 km did not induce any significant changes in blood cellular components and biomarker of oxidative stress, but levamisole improved erythrocyte membrane stability. Ivermectin was administered at a dose rate of 0.2 mg /kg per os and Levamisole at a dose rate of 7.5 mg/kg per os for the helminth efficacy study. Levamisole efficacy treatment group had a reduction test of 86.8% while Ivermectin efficacy study group had 76%. This may indicate occurrence of resistance to both drugs in the donkeys. Further research is needed in order to obtain the efficacy of this drugs in a larger population There is a need to develop better and improved management practices to combat and ameliorate the challenge that helminthosis in donkeys posses to the health and working performance of donkeys.
Radical antegrade modular pancreatosplenectomy (RAMPS) versus standard retrograde pancreatosplenectomy (SRPS) for resectable body and tail pancreatic adenocarcinoma: protocol of a multicenter, prospective, randomized phase III control trial (CSPAC-3)
Authors: Li Jialin , Shi Si , Liu Jiang , Liang Chen , Hua Jie , Meng Qingcai , Xu Hang , Wei Miaoyan , Zhang Bo , Xu Jin , Wang Wei , Yu Xianjun
Published: January 2023

Background

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy. Radical surgical resection offers the only potential cure. There is increasing agreement that radical antegrade modular pancreatosplenectomy (RAMPS) may benefit patients with tumors in the body and tail of the pancreas. To address this, the Chinese Study Group for Pancreatic Cancer (CSPAC) − 3 trial is proposed to compare the effect of RAMPS and standard retrograde pancreatosplenectomy (SRPS) on patient survival and preoperative safety

Methods

The randomized controlled trial will be multicenter and two-armed with blinded outcomes and intention-to-treat analysis. Three hundred patients with resectable body and tail pancreatic adenocarcinoma will be enrolled and randomly assigned to RAMPS or SRPS. Adjuvant chemotherapy based on an initial regimen will be recommended 4–6 weeks after surgery if no serious complication occurs. The hypothesis that RAMPS improves survival outcome compared with SRPS will be tested using a superiority trial. The primary outcome will be overall survival (OS). Secondary outcomes will include recurrence-free survival (RFS), R0 resection rate, the number of harvested lymph nodes, post-operative complications, and quality of life scores.

Discussion

The use of RAMPS has increased over the past decade. It is reported that RAMPS is superior to SRPS in improving both the rate of R0 resection and lymph node yield. Despite these advantages, however, there is little high-level documentation of the superiority of RAMPS in terms of survival and this needs to be investigated. To address this issue, CSPAC has instigated the first prospective, randomized phase III control trials, aiming to explore the optimal surgical strategy for improving the prognosis and OS of patients with left-sided pancreatic cancer

Trial registration

Trial registration number ChiCTR2100053844; Pre-results; Date of registration December 1, 2021
Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis
Authors: Yu Yiming , Ozaki Takuma , Yoshimura Shige
Published: January 2023

Clathrin-mediated endocytosis plays a pivotal role in signal transduction pathways between the extracellular environment and the intracellular space. Accumulating evidence from live-cell imaging and super-resolution microscopy of mammalian cells suggests an asymmetric distribution of actin fibres near the clathrin-coated pit, which induces asymmetric pit-closing, rather than radial constriction. However, detailed molecular mechanisms of this “asymmetricity” remain elusive. Herein, we used high-speed atomic force microscopy to demonstrate that CIP4, a multidomain protein with a classic F-BAR domain and intrinsically disordered regions, is necessary for asymmetric pit-closing. Strong self-assembly of CIP4 via intrinsically disordered regions, together with stereospecific interactions with the curved membrane and actin-regulating proteins, generates a small actin-rich environment near the pit, which deforms the membrane and closes the pit. Our results provide a mechanistic insight into how spatio-temporal actin polymerization near the plasma membrane is promoted by a collaboration of disordered and structured domains.
Article Exploring Economic and Environmental Efficiency in Renewable Energy Utilization: A Case Study in The Organization for Economic Cooperation and Development Countries
Authors: Wang Chia-Nan , Nguyen Thi Thanh Tam , Dang Thanh-Tuan , Hsu Hsien-Pin
Published: January 2023

The share of renewable energy has grown significantly in global energy supply, yet the evaluation of renewable energy utilization has remained limited. Hence, this research explored economic and environmental efficiency in renewable energy utilization in 20 OECD (The Organization for Economic Cooperation and Development) countries from 2015 to 2020. The study approached the Data Envelopment Analysis - Slacks-Based Measure (DEA-SBM) to evaluate economic efficiency, and the DEA undesirable output to measure environmental efficiency. Four inputs as labor force, gross capital formation, total renewable energy capacity, and share of renewable energy were compared to two desirable outputs as the gross domestic product (GDP), and total energy production in the economic efficiency model. In the environmental efficiency model, the same inputs were evaluated with GDP as the desirable output and CO2 emissions as the undesirable output. The DEA-SBM revealed that Australia, Belgium, Norway, the United Kingdom, and the United States were efficient in economic evaluation whereas, DEA undesirable output indicated that Belgium, France, Sweden, the United Kingdom, and the United States were efficient in environmental evaluation. The governments are suggested to adjust the resources management since the number of efficient units decreased in the economic assessment while fluctuating in the environmental assessment.
Novel Compound Heterozygous CYBA Mutations Causing Neonatal-Onset
Authors: Zhang Liang , Yu Lang , Li Junshuai , Peng Yu , He Siping , Huang Ruiwen , Li Zhihui , Zhao Xiaodong
Published: January 2023

Chronic granulomatous disease (CGD) is a rare inborn error of immunity (IEI) characterized by a defective respiratory burst by phagocytes and defective clearance of phagocytosed microorganisms; these phenomena, caused by a defect in NADPH oxidase, result in severe and life-threatening infections in affected children. The genetically heterogeneous X-linked recessive (XL-CGD) form of GCD is caused by mutations in the CYBB gene, whereas the autosomal recessive (AR-CGD) form is caused by mutations in the CYBA, NCF1, NCF2, NCF4, or CYBC1 genes. Mutations in the CYBA gene account for a small number of CGD cases; the vast majority of these patients become symptomatic in childhood, but rarely within the first weeks of life. Here, we report a 19-day-old neonate who developed pustular rashes and invasive pulmonary aspergillosis, which was identified by a galactomannan (GM) assay of both bronchoalveolar lavage fluid (BALF) and peripheral blood samples, and by metagenomic next-generation sequencing (mNGS) of BALF. A diagnosis of CGD was based on the respiratory burst test. Detailed assessment of neutrophil activity revealed that production of reactive oxygen species (ROS) was entirely absent. Whole-exome sequencing (WES) detected a nonsense mutation (c.7G>T). In addition, copy number variation (CNV) analysis detected a novel de novomicrodeletion of 200 kb at 16q24.2-q24.3. Thus, we have identified novel compound heterozygous CYBA mutations that cause neonatal AR-CGD, thereby expanding the clinical spectrum of CYBA deficiency.
Precise Fourier series and fuzzification method analysis of Standardized thermal Energy of Solar box cooker performance: Economic, Environmental study
Authors: Bhavani Sundararajan , Nandini Karthik , Muthucumaraswamy Rajamanickam , Chithambaram Venkatesan , Shanmugan Sengottaiyan
Published: January 2023

A solar cooker is indispensable for preparing nutritious foodstuff than the food prepared by LPG, which is evitable. In the present scenario, the solar cooker incorporated with the coating of nanoparticles led to increased thermal performance and reduced cooking time. An attempt has been made to use the Fourier series to forecast the thermal performance and validate the Fourier series analysis of cookery liquid temperatures. Also, Fuzzification entire cookery process with the climatic parameters has been modeled logically for decision-making and to draft fuzzy rules for the cooking process. Further, during the experimentation, the cooking process has undergone a thermal analysis to validate the fuzzy inference. From the study, it is observed that both the Fourier series analysis and fuzzy logic modeling are intact with experimental observation and can be utilized for the simulation of the proposed system in various climatic conditions similar to that of the local climatic conditions in Chennai. A solar box cooker studies of environmental and economical is applied to calculate total carbon dioxide emissions (%) reduce a period when this design was utilized in contrast to Pt values that have higher dependence, which is a direct relationship with minimization of CO2 emission. The overall efficiency during boiling water is found to be 52.6% and 38.99% during the steady state temperature of the oil.
Outcome of Silicone Sling Frontalis Suspension in Isolated Uncomplicated Congenital Ptosis vs. Complicated Ptosis
Authors: Prat Daphna Landau , Zhao Cindy , Ramakrishnan Meera , Revere Karen , Katowitz William , Katowitz James
Published: January 2023

Objectives
To compare the outcome of silicone sling frontalis suspension (FS) surgery in children with isolated uncomplicated congenital ptosis (IUCP) vs. children with complicated ptosis.
Methods
A retrospective medical chart review of all pediatric patients who underwent silicone sling FS surgery between 2009–2020 at a single center were included. Patients were divided according to ptosis type: IUCP vs. complicated ptosis. Pre-and post-operative margin-to-reflex distance (MRD1) measurements were determined from clinical photographs. Main outcome measures were differences in improvement in eyelid height, reoperation rate, and timing between the groups.
Results
Two-hundred and eight children were included: 139 IUCP and 69 complicated cases, 83 females (40%), mean (± SD) age at intervention was 1.9 ± 2.9 years. Complicated cases included: blepharophimosis epicanthus inversus syndrome (n = 35), Marcus Gunn jaw winking (n = 12), oculomotor palsy (n = 8), congenital fibrosis of extraocular muscles (n = 3), chronic progressive external ophthalmoplegia (n = 3), and others. Mean MRD1 improved by an average of 1.6 mm in both groups. Repeat ptosis repair was performed in 50/171 (29%) patients without a history of failed ptosis procedures, and this rate was similar between IUCP and complicated cases. Children under 3 years of age had higher rates of repeat ptosis repair than older children (n = 59/175, 34% vs n = 5/33, 15%, p = 0.03 chi2).
Conclusions
Silicone sling frontalis suspension has a favorable outcome in 70% of pediatric patients. Preoperative and final MRD1 and reoperation rates were similar between both groups, suggesting that despite the higher complexity in atypical cases, the outcome is similar.
Identifying s-wave pairing symmetry in single-layer FeSe from topologically trivial edge states
Authors: Wei Zhongxu , Qin Shengshan , Ding Cui , Hu Jiangping , Sun Yu-jie , Wang Lili , Xue Qi-Kun
Published: January 2023

Determining the pairing symmetry of single-layer FeSe on SrTiO3 is the key to understanding the enhanced pairing mechanism; furthermore, it guides exploring new superconductors with high transition temperatures (Tc). Despite significant efforts, it remains controversial whether the symmetry is the sign-preserving s- or the sign-changed s±-wave. Here, we investigate the pairing symmetry of single-layer FeSe from a topological point of view. Using low-temperature scanning tunneling microscopy/spectroscopy, we have systematically characterized the superconducting states at isolated edges and corners of single-layer FeSe. The tunneling spectra collected at edges and corners exhibit full gap and substantial dip, respectively, demonstrating the absence of topologically non-trivial edge/corner modes. According to the theoretical calculation, these spectroscopic features are strong evidence for the sign-preserving s-wave pairing.
An integrated primary care service to reduce cardiovascular disease risk in people with severe mental illness: Primrose-A. Thematic analysis of its acceptability, feasibility, and implementation.
Authors: Shaw Philippa , Mifsud Annabel , Osborn David , Nahata Nitisha , Hall Cerdic , Prenelle Ian , Lamb Danielle
Published: January 2023

Background
Cardiovascular disease among patients with severe mental illness in England is a major preventable contributor to premature mortality. To address this, a nurse and peer-coach delivered service (Primrose-A) was implemented in three London general practices from 2019 (implementation continued during COVID-19). This study aimed to conduct interviews with patient and staff to determine the acceptability of, and experiences with, Primrose-A.
Methods
Eight patients who had received Primrose-A, and 3 nurses, 1 GP, and 1 peer-coach who had delivered Primrose-A in three London-based GP surgeries. Semi-structured audio-recorded interviews were conducted. Reflexive thematic analysis was used to identify themes from the transcribed interviews.
Findings
Overall, Primrose-A was viewed positively by patients and staff, with participants describing success in improving patients’ mental health, isolation, motivation, and physical health. Therapeutic relationships between staff and patients, and long regular appointments were important facilitators of patient engagement and acceptance of the intervention. Several barriers to the implementation of Primrose-A were identified, including training, administrative and communication issues, burden of time and resources, and COVID-19.
Conclusions
Intervention acceptability could be enhanced by providing longer-term continuity of care paired with more peer-coaching sessions to build positive relationships and facilitate sustained health behaviour change. Future implementation of Primrose-A or similar interventions should consider: 1) training sufficiency (covering physical and mental health, including addiction), 2) adequate staffing to deliver the intervention, 3) facilitation of clear communication pathways between staff, and 4) supporting administrative processes.
A Peptide Barcode Based Approach for Efficient in vivo Screening of RNA Delivery Systems
Authors: Odunze Uchechukwu , Rustogi Nitin , Devine Paul , Miller Lorraine , Pereira Sara , Vashist Surender , Snijder Harm , Corkill Dominic , Sabirsh Alan , Douthwaite Julie , Bond Nick , Desai Arpan
Published: January 2023

The clinical advancement of mRNA therapeutics for diverse applications, facilitated by lipid nanoparticles (LNPs), has demonstrated the promise of LNPs as a delivery vector. Continued exploration of LNPs for application in new disease areas requires identification and optimisation of leads in a high throughput way. Currently available high throughput in vivo delivery system screening platforms are better suited to screen for cellular uptake but less so for functional cargo delivery. We have therefore developed a Proteomics Associated LNP Screening (PALS) platform which measures functional delivery of LNPs using unique peptide ‘barcodes’. We report on the design and selection of the peptide barcodes and the evaluation of these barcodes for the screening of LNPs. We show that proteomic analysis of peptide barcodes correlates with quantification and efficacy of barcoded reporter proteins both in vitro and in vivo and, that the ranking of selected LNPs using peptide barcodes in a pool correlates with ranking using alternative methods in groups of animals treated with individual LNPs. We show that this system is sensitive, selective, and capable of reducing the size of an in vivo study by at least 10-fold, thus accelerating the discovery of new technologies for mRNA delivery.
A XGBoost predictive model of reproductive outcomes in patients following hysteroscopic adhesiolysis
Authors: Li Yazhu , Duan Hua , Wang Sha
Published: January 2023

Background
Infertility is the primary clinical symptom and reason for visiting patients with intrauterine adhesions (IUAs). Exploring the factors influencing the prognosis of IUAs and establishing a predictive model for reproductive outcomes after hysteroscopic adhesiolysis (HA) are extremely important for the selection of clinical treatment and prognostic assessment.
Methods
The clinical informations of 369 individuals diagnosed with and treated for IUAs were obtained from the Intrauterine Adhesion Multicenter Prospective Clinical Database (IUADB, NCT05381376) and randomly divided into the training and validation cohorts. A univariate analysis was performed to identify relevant clinical indicators, followed by a least absolute shrinkage and selection operator (LASSO) regression for regularization and SHapley Additive exPlanation (SHAP) for extreme gradient boosting (XGBoost) predictive model visualization. Finally, receiver operating characteristic (ROC) curves were constructed to assess the model’s efficiency.
Results
Univariate analysis and LASSO regression demonstrated that 12 clinical indicators were significantly associated with postoperative reproductive outcomes in IUAs patients. SHAP visualization indicated that postoperative fallopian tube ostia, blood supply, uterine cavity shape and age had the highest significance. The area under the ROC curve (AUC) of the XGBoost model in the training and validation cohorts was 0.987 (95% CI 0.9787–0.996) and 0.9851 (95% CI 0.9668-1), respectively. These values were significantly higher than those of the American Fertility Society (AFS) classification, the Chinese Society for Gynaecological Endoscopy (CSGE) classification and endometrial thickness (all P < 0.01).
Conclusions
The XGBoost model had higher accuracy in predicting postoperative reproductive outcomes in IUAs patients. Clinically, our model may be useful for managing and categorizing IUAs and determining optimal action to aid in pregnancy.
Trial registration:
The study was an observational cohort study, and the data were obtained from the Chinese Uterine Adhesion Database (ClinicalTrials.gov; NCT05381376; 19/05/2022).
A fabricated hydrogel of hyaluronic acid / curcumin shows super-activity to heal the bacterial infected wound
Authors: Khaleghi Maryam , Haghi Fakhri , Gholami Mina , Hoorfar Hamdam , Shahi Farshad , Zekoloujeh Ali Mir Mousavi , Aliakbari Farhang , Ahmadi Ebrahim , Morshedi Dina
Published: January 2023

High risk of acute morbidities and even mortality from expanding the antibiotics resistant infectious wounds force indefinite efforts for development of high performance wound-healing materials. Herein, we design a procedure to fabricate a hyaluronic acid (HA)-based hydrogel to conjugate curcumin (Gel-H.P.Cur). The highlight of this work is to provide a favorite condition for capturing curcumin with protecting its structure and intensify its activities because of the synchronization with HA. Accordingly, HA as a major component of dermis with critical role in establishing skin health, could fortify the wound healing property as well as antibacterial activity of the hydrogel. Gel-H.P.Cur showed antibacterial properties against Pseudomonas aeruginosa (P. aeruginosa), which were examined by bactericidal efficiency, disk diffusion, anti-biofilm, and pyocyanin production assays. The effects of Gel-H.P.Cur on the inhibition of quorum sensing (QS) regulatory genes that contribute to expanding bacteria in the injured place was also significant. In addition, Gel-H.P.Cur showed high potential to heal the cutaneous wounds on the mouse excisional wound model with repairing histopathological damages rapidly and without scar. Taken together, the results strongly support Gel-H.P.Cur as a multipotent biomaterial for medical applications regarding the treatment of chronic, infected, and dehiscent wounds.
Sex-Specific Optimal Target Blood Pressure for the Primary Prevention of Hemorrhagic Stroke: A Nationwide Observational Study
Authors: Shim Hwan Seok , Park Jeong Mee , Lee Yong Jae , Kim Young Deok , Kim Tackeun , Ban Seung Pil , Bang Jae Seung , Kwon O-ki , Oh Chang Wan , Lee Si Un
Published: January 2023

There arefew reports on the preventative value of intensive blood pressure (BP) management for stroke, especially hemorrhagic stroke (hSTK),after new criteria for hypertension (HTN) were announced by theAmerican College of Cardiology/American Heart Association in 2017. To identify the optimal BP for the primary prevention of hSTK in a healthy population aged between 20 and 65 years, we conducted a 10-yearobservational study on the risk of hSTK, subclassified as intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SAH) according to BP categories (e.g.,low normal BP, high normal BP, elevated BP, stage 1 HTN and stage 2 HTN) using the National Health Insurance Service Database. Of the 8,325,579 participants who had a health checkup in 2008, 947,378 were ultimately enrolled in this study and observed from 2009 to 2018. The risk of ICH was significantly increased from stage 2 HTN (adjusted hazard ratio (AHR) 2.002 [95% confidence interval (CI) 1.203-3.332]) and stage 1 HTN (AHR 2.021 [95% CI, 1.251-3.263]) in men and women, respectively. The risk of SAH was significantly increased among both men (AHR 1.637 [95% CI, 1.066-2.514]) and women (AHR 4.217 [95% CI, 2.648-6.715]) from stage 1 HTN. The risk of hSTK was significantly increased among men with stage 2 HTN (AHR 3.034 [95% CI, 2.161-4.260]) and women with stage 1 HTN (AHR 2.976 [95% CI, 2.222-3.986]).Therefore, for the primary prevention of hSTK, including ICH and SAH, adults under the age of 65 should manage their BP from stage 1 HTN.
Expanding The Role of PSMA PET for Active Surveillance
Authors: Jain Anika , Nassour Anthony-Joe , Dean Thomas , Patterson Imogen , Tarlinton Lisa , Kim Lawrence , Woo Henry
Published: January 2023

Introduction:
Accurate grading at the time of diagnosis is fundamental to risk stratification and treatment decision making, particularly for men being considered for Active Surveillance (AS). With the introduction of prostate-specific membrane antigen (PSMA) positron emission tomography (PET) there has been considerable improvement in sensitivity and specificity for the detection and staging of clinically significant prostate cancer. Our study aims to determine the role of PSMA PET/CT in men with newly diagnosed low or favourable intermediate risk prostate cancer to better select men for AS.
Method:
This is a retrospective single centre study performed from January 2019 and October 2022. This study includes men identified from electronic medical record system who had undergone a PSMA PET/CT following newly diagnosed low or favourable-intermediate risk prostate cancer. Primary outcome was to assess the change in management for men being considered for AS following PSMA PET/CT results on the basis of PSMA PET characteristics. The study is approved (AHCL/HREC/2022-008) by the Adventist HealthCare Limited (AHCL) Human Research Ethics Committee (HREC).
Results:
In total, there were 11 of 30 men (36.67%) who were assigned management by AS and 19 of 30 men (63.33%) who required further treatment. 15 of the 19 men that needed treatment had concerning features on PSMA PET/CT results. Of the 15 men with concerning features on PSMA PET, 9 (60%) men were found to have adverse pathological features on final prostatectomy features.
Conclusion:
This retrospective study suggests that PSMA PET/CT has potential to influence the management of men with newly diagnosed prostate cancer that would otherwise be appropriate for active surveillance.
The effects of exercise therapy feedback on subjective treatment outcome and patient satisfaction: study protocol for a mono-centric, randomized, controlled trial in orthopedic rehabilitation (FeedYou)
Authors: Schuber André Arik , Schmidt Sebastian , Hombach Sarah , Schaller Andrea
Published: January 2023

Background: The disease burden of musculoskeletal disorders necessitates multidisciplinary and patient-centered models of care. Exercise therapy represents a first-line treatment strategy and a central component of medical rehabilitation. In order to realize the goals of long-term physical activity and participation as proposed by the ICF, exercise therapy can be supplemented by interventional techniques from the field of psychotherapy. Although psychotherapist feedback has been shown to improve therapeutic outcome and patient satisfaction, feedback use in exercise therapy is mostly limited to motor learning and exercise instruction. The present paper therefore describes the use of multidimensional exercise therapy feedback in medical rehabilitation. The aims of the trial presented in this study protocol are to evaluate the effects of this novel feedback approach on rehabilitation outcomes in comparison to usual care.
Methods: The study is designed as a prospective, mono-centric, randomized controlled, superiority trial (RCT) with two parallel groups and three measuring points: T0 = start of three-week inpatient rehabilitation, T1 = end of three-week inpatient rehabilitation, T2 = 12-week follow-up. In total, 132 patients suffering from chronic neck, shoulder and/or lumbar spine disorders will be recruited. The intervention involves multidimensional exercise therapy feedbacks during the initial and final physical therapist examination, as well as short exercise therapy feedbacks during the course units of the mandatory group-based exercise therapy program. Primary outcomes are the subjective treatment outcome, assessed by BPI and indication-specific questionnaires, as well as patient satisfaction, assessed by ZUF-8 and an intervention-specific questionnaire. The final data collection is expected by May 2023.
Discussion: This study may provide a valuable insight into the effectiveness of multidimensional exercise therapy feedback to improve treatment outcomes and patient satisfaction in medical rehabilitation. This could contribute to rehabilitation quality assurance and the long-term physical activity behavior of rehabilitation patients. Trial registration: The trial has been registered with the German Clinical Trial Register (DRKS) under the registration number DRKS00027263.
Trial registration: The trial has been registered with the German Clinical Trial Register (DRKS) under the registration number DRKS00027263.
Identification and validation of RAB2A as a new prognostic biomarker for breast cancer
Authors: Lin Shenguo , Mao Yiwen , Hu Chengda , Lv Rui , Zeng Hongdou , Zhang Minghao , Wu Xinlei , Lin Lizi , Ouyang Shanshan , Zhao Yaxin
Published: January 2023

Background: Targeting GTPase 2A(RAB2A) is overexpressed in a variety of malignancies and plays an essential role in tumor formation. However, the predictive significance of RAB2A in breast cancer remains undetermined.
Methods:In this study, breast cancer patients' expression profiles and clinical data were extracted from The Cancer Genome Atlas (TCGA) database. Using the Wilcoxon rank sum test, RAB2A expression levels were compared between breast cancer and normal breast tissue. An enrichment analysis was performed to investigate potential signaling pathways and associated biological activities. Using single-sample gene set enrichment analysis, the invasion of immune cells was evaluated. Utilizing Ualcan and the MethSurv database, the methylation status of RAB2A was determined. Using the Kaplan-Meier technique and Cox regression analysis, the prognostic value of RAB2A was determined. A nomogram was developed to predict overall survival (OS) one, three, and five years following cancer diagnosis.
Results: RAB2A was substantially linked with higher PR, ER status, N grade, histological type, PAM50, and age and was overexpressed in breast cancer.Overexpression of RAB2A significantly decreased overall survival and disease-specific survival. RAB2A was found as an independent negative predictive factor for OS by multivariate Cox analysis. The concordance index of the OS nomogram generated was 0.72. Similarly, RAB2A hypomethylation was related with a bad outcome. Analysis of functional enrichment revealed that keratinocyte development, humoral immune response pathway, IL-16 signaling pathway, cytokine-receptor interactions, immune response regulatory signaling pathway, and taste transduction were among the enriched pathways. In addition, RAB2A overexpression was negatively linked with the number of necrotizing cells, CD8+ T cells, dendritic cells, and plasmacytoid dendritic cells that infiltrated the tissue.
Conclucion:RAB2A may serve as a possible breast cancer prognostic biomarker and therapeutic target. Our findings establish the groundwork for future research into the molecular mechanisms of RAB2A in breast cancer and are essential for clarifying RAB2A's therapeutic relevance in breast cancer.