bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of dietary supplementation of pioglitazone or walnut meal on metabolic profiles and oxidative status in dairy cows with high pre-calving BCS
Authors: Jamali Emam Gheise Negin , Riasi Ahmad , Celi Pietro , Shahneh Ahmad Zare
Published: February 2018

This research paper addresses the hypothesis that dietary pioglitazone (PGT), as synthetic and specific ligand for PPAR-γ or walnut meal (WM) as a natural ligand for PPAR-γ, affect plasma metabolites and reduce the oxidative status in high body condition score (BCS) dairy cows (≥4 BCS). Total of 36 multiparous Holstein cows were randomly assigned to one of the dietary treatments: 1- Control (basal diet; CTR), 2- Walnut meal (9·45% walnut meal of DMI; WM), and 3- Pioglitazone (6 mg/kg BW; PGT). The experimental diets were fed from parturition time to 21 d postpartum. Results showed that the PGT supplementation increased dry matter intake (DMI) (22·95 kg/d) compared to the CTR (21·45 kg/d) and WM (21·78 kg/d) groups. Results showed that milk yield and milk composition were not affected by the experimental diets. Body condition score losses tended to be higher in the CTR group compared to the PGT and WM cows. The PGT group had higher plasma insulin compared to the CTR group (11·84 vs. 10·68 mIU/l), and WM cows had intermediate plasma insulin. The PGT cows had lower plasma non esterified fatty acid (NEFA) and tended to have lower β-hydroxy butyric acid (BHBA) than the CTR group. Feeding pioglitazone decreased plasma malondialdehyde (MDA) and increased plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD) compared to the CTR and WM groups. It was concluded that dietary pioglitazone had positive effects on DMI, BCS change, blood metabolites and oxidative status in fresh dairy cows with high pre-calving BCS. The anti-oxidant effects of walnut meal were not supported by the present data.
Bovine spongiform encephalopathy (BSE) associated polymorphisms of the prion-like protein gene (PRND) in Korean dairy cattle and Hanwoo
Authors: Kim Yong-Chan , Jeong Byung-Hoon
Published: February 2018

Bovine spongiform encephalopathy (BSE) involves insertion/deletion (in/del) polymorphisms in the prion protein gene (PRNP) promoter region that are associated with vulnerability to disease progression. Recently, a second member of the prion gene family, prion-like protein gene (PRND), has been reported to show the PRND R132Q polymorphism, which is associated with the susceptibility to BSE in German Fleckvieh breeds. The objective of this study was to examine the genotype, allele, and haplotype frequencies of PRND gene in Korean cattle and evaluate their susceptibility to BSE. We did this in 277 Korean native cattle (Hanwoo) and 124 Korean dairy cattle (Holstein) by direct sequencing and compared the R132Q genotype frequency between BSE-affected German cattle and Korean cattle. The results indicated a total of 5 single nucleotide polymorphisms (SNPs) including PRND c.149G > A (p.50Arg > His; R50H), PRND c.285C > T (C4819T), PRND c.395G > A (p.132Arg > Gln; R132Q) and PRND c.528T > A (T5063A) in the open reading frame (ORF) and c.602C > G in the 3′ untranslated region (UTR) of exon 2 in Korean Holstein and Hanwoo cattle. Except for c.149G > A, the remaining 4 SNPs showed significantly different genotype and allele frequencies between the Korean Holstein and Hanwoo (P < 0·01). There were no significant differences in genotype distribution of c.395G > A SNP between BSE-affected German and Korean Holstein cattle (P = 0·6778), but a significant difference was detected between BSE-affected German cattle and Hanwoo cattle (P = 0·0028). The results suggest that Hanwoo cattle may possess a relatively more BSE-resistant genotype than Korean Holstein cattle.
Effect of heat-induced κ-casein dissociation on acid coagulation of milk
Authors: Oka Daiki , Ono Wataru , Ohara Shintarou , Noguchi Tomohiro , Takano Katsumi
Published: February 2018

In this study, the relationship between the dissociation of κ-casein from casein micelles due to heat-induced denaturation and the strength of acid milk gel was investigated. The κ-casein-dissociated micelles were fractionated by gel filtration chromatography and two-dimensional polyacrylamide gel electrophoresis, and their zeta potential and surface hydrophobicity were measured. The negative charge of the κ-casein-dissociated micelles was lower than that of native micelles, and micellar surface hydrophobicity was higher. For confirmation, the isoelectric point of the casein micelles was measured. The κ-casein-dissociated micelles were found to cohere at an earlier stage of acidification than the native micelles. These results demonstrated that the heat-induced increase in the strength of acid milk gel was partly due to the decrease in micellar surface charge and partly to the increase in surface hydrophobicity caused by the dissociation of κ-casein.
Indirect visualisation of the upper pole during tonsillectomy
Authors: Walijee H , Krishnan M , Sharma R , De S
Published: February 2018

Background:

During tonsillectomy, one of the key concerns is establishing adequate haemostasis. Intra-operative haemorrhage from the upper pole of the tonsil fossae can be a challenge to deal with because of an inability to clearly visualise bleeding points. Laryngeal mirrors are readily available in an ENT operating theatre and routinely used for indirect visualisation of the post-nasal space during adenoidectomy.

Objective:

This paper describes a technique, utilising a laryngeal mirror placed within the tonsil fossa (after having removed the palatine tonsil), to visualise the upper pole and any bleeding points.

Results and conclusion:

Laryngeal mirrors can be used with case to inspect the tonsil fossa post-tonsillectomy for bleeding points which may not be directly visible, so that these can be cauterised.
Word meaning in word identification during reading: Co-occurrence-based semantic neighborhood density effects
Authors: AL FARSI BADRIYA H.
Published: February 2018

Identifying individual words is an essential part of the reading process that should occur first so that understanding the structural relations between words and comprehending the sentence as a whole may take place. Therefore, lexical processing (or word identification) has received much attention in the literature, with many researchers exploring the effects of different aspects of word representation (orthographic, phonological, and semantic information of words) in word identification. While the influence of many orthographic and phonological factors in normal reading are well researched and understood (Rayner, 1998, 2009), the effect of semantic characteristics of a word in its identification has received relatively less attention. A complete account of lexical processing during normal reading requires understanding the role of word meaning in lexical processing. Currently, little is understood about whether and how the meaning of an individual word is extracted during early stages of word identification. This article primarily focuses on how word meaning contributes to the process of word identification.
Agreement attraction in native and nonnative speakers of German
Authors: LAGO SOL , FELSER CLAUDIA
Published: February 2018

Second language speakers often struggle to apply grammatical constraints such as subject–verb agreement. One hypothesis for this difficulty is that it results from problems suppressing syntactically unlicensed constituents in working memory. We investigated which properties of these constituents make them more likely to elicit errors: their grammatical distance to the subject head or their linear distance to the verb. We used double modifier constructions (e.g.,the smellof the stables of the farmers), where the errors of native speakers are modulated by the linguistic relationships between the nouns in the subject phrase: second plural nouns, which are syntactically and semantically closer to the subject head, elicit more errors than third plural nouns, which are linearly closer to the verb (2nd-3rd-noun asymmetry). In order to dissociate between grammatical and linear distance, we compared embedded and coordinated modifiers, which were linearly identical but differed in grammatical distance. Using an attraction paradigm, we showed that German native speakers and proficient Russian speakers of German exhibited similar attraction rates and that their errors displayed a 2nd-3rd-noun asymmetry, which was more pronounced in embedded than in coordinated constructions. We suggest that both native and second language learners prioritize linguistic structure over linear distance in their agreement computations.
Human Images and Blurring Boundaries. The Pueblo Body in Cosmological Context: Rock Art, Murals and Ceremonial Figures
Authors: Schaafsma Polly
Published: February 2018

Images of the human form can be analysed for what they reveal about social roles, hierarchy, and other identities, as well as culturally determined perceptions about humanity's relationships to the natural environment and supernatural realm. It is proposed that the portrayal of the multitudinous human subjects related to religious ideology and practice in Rio Grande Tradition and Navajo rock art focuses on the interconnectedness of all things, deflecting meaning away from human beings as prime subjects as seen in Western religious art. Rather, informed by ethnographic data, the Native American abstracted, costumed forms, along with conflated human/animal subjects, define humanity's intimate link to the cosmos, and their added attributes evoke the supernatural strengths of other living beings, along with animated entities such as rain-clouds and the sun. These images themselves are perceived as active agents, attracting the pictured forces, sanctifying place and facilitating communication with resident spirits. What is pictured on stone extends to the performative dimensions of ethnographic contexts, thereby blurring the boundaries between the ceremonial participants, the representations and the animistic cosmos.
Interviewer effects in food acquisition surveys
Authors: Ong Ai Rene , Hu Mengyao , West Brady T , Kirlin John A
Published: February 2018

Objective

To understand the effects of interviewers on the responses they collect for measures of food security, income and selected survey quality measures (i.e. discrepancy between reported Supplemental Nutrition Assistance Program (SNAP) status and administrative data, length of time between initial and final interview, and missing income data) in the US Department of Agriculture’s National Household Food Acquisition and Purchase Survey (FoodAPS).

Design

Using data from FoodAPS, multilevel models with random interviewer effects were fitted to estimate the variance in each outcome measure arising from effects of the interviewers. Covariates describing each household’s socio-economic status, demographics and experience in taking the survey, and interviewer-level experience were included as fixed effects. The variance components in the outcomes due to interviewers were estimated. Outlier interviewers were profiled.

Setting

Non-institutionalized households in the continental USA (April 2012–January 2013).

Subjects

Individuals (n 14 317) in 4826 households who responded to FoodAPS.

Results

There was a substantial amount of variability in the distributions of the outcomes examined (i.e. time between initial and final interview, reported values for food security, individual income, missing income) among the FoodAPS interviewers, even after accounting for the fixed effects of the household- and interviewer-level covariates and removing extreme outlier interviewers.

Conclusions

Interviewers may introduce error in food acquisition survey data when they are asked to interact with the respondents. Managers of future surveys with similarly complex data collection procedures could consider using multilevel models to adaptively identify and retrain interviewers who have extreme effects on data collection outcomes.
Molecular serogrouping ofEscherichia coli
Authors: DebRoy Chitrita , Fratamico Pina M. , Roberts Elisabeth
Published: February 2018

O-antigens present on the surface ofEscherichia coliprovide antigenic specificity for the strain and are the main components for O-serogroup designation. Serotyping using O-group-specific antisera for the identification ofE. coliO-serogroups has been traditionally the gold-standard for distinguishingE. colistrains. Knowledge of the O-group is important for determining pathogenic lineage, classifyingE. colifor epidemiological studies, for determining virulence, and for tracing outbreaks of diseases and sources of infection. However, serotyping has limitations, as the antisera generated against each specific O-group may cross-react, many strains are non-typeable, and others can autoagglutinate or be rough (lacking an O-antigen). Currently, the nucleotide sequences are available for most of the 187 designatedE. coliO-groups. Public health and other laboratories are considering whole genome sequencing to develop genotypic methods to determine O-groups. These procedures require instrumentation and analysis that may not be accessible and may be cost-prohibitive at this time. In this review, we have identified unique gene sequences within the O-antigen gene clusters and have targeted these genes for identification of O-groups using the polymerase chain reaction. This information can be used to distinguish O-groups by developing other platforms forE. colidiagnostics in the future.
Why policy needs history (and historians)
Authors: Berridge Virginia
Published: February 2018

Policy makers like the idea of new initiatives and fresh starts, unencumbered by, even actively overthrowing, what has been done in the past. At the same time, history can be pigeonholed as fusty and antiquarian, dealing with long past events of no relevance to the present. Academic historians are sometimes bound up in their own worlds. The debates central to academe may have little direct relevance to the immediate concerns of policy making. The paper argues that history, as the evidence-based discipline par excellence, is as relevant as other approaches to evidence-based policy making. Case studies can show us the nature of that relevance. How to achieve influence for history also needs discussion. The relationship is not straightforward and will vary according to time and place. History is an interpretative discipline, not just a collection of ‘facts’. The paper discusses how historians work and why it is important for policy makers to engage, not just with history, but with historians as well. Historians too need to think about the value of bringing their analysis into policy.
Weed Management in 2050: Perspectives on the Future of Weed Science
Authors: Westwood James H. , Charudattan Raghavan , Duke Stephen O. , Fennimore Steven A. , Marrone Pam , Slaughter David C. , Swanton Clarence , Zollinger Richard
Published: February 2018

The discipline of weed science is at a critical juncture. Decades of efficient chemical weed control have led to a rise in the number of herbicide-resistant weed populations, with few new herbicides with unique modes of action to counter this trend and often no economical alternatives to herbicides in large-acreage crops. At the same time, the world population is swelling, necessitating increased food production to feed an anticipated 9 billion people by the year 2050. Here, we consider these challenges along with emerging trends in technology and innovation that offer hope of providing sustainable weed management into the future. The emergence of natural product leads in discovery of new herbicides and biopesticides suggests that new modes of action can be discovered, while genetic engineering provides additional options for manipulating herbicide selectivity and creating entirely novel approaches to weed management. Advances in understanding plant pathogen interactions will contribute to developing new biological control agents, and insights into plant–plant interactions suggest that crops can be improved by manipulating their response to competition. Revolutions in computing power and automation have led to a nascent industry built on using machine vision and global positioning system information to distinguish weeds from crops and deliver precision weed control. These technologies open multiple possibilities for efficient weed management, whether through chemical or mechanical mechanisms. Information is also needed by growers to make good decisions, and will be delivered with unprecedented efficiency and specificity, potentially revolutionizing aspects of extension work. We consider that meeting the weed management needs of agriculture by 2050 and beyond is a challenge that requires commitment by funding agencies, researchers, and students to translate new technologies into durable weed management solutions. Integrating old and new weed management technologies into more diverse weed management systems based on a better understanding of weed biology and ecology can provide integrated weed management and resistance management strategies that will be more sustainable than the technologies that are now failing.
Lack of Association Between Type 2 Diabetes and the 3673G / A and 9041G / A Gene Variants of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1)
Authors: Ozbayer Cansu , Kurt Hulyam , Nur Kebapci Medine , Turgut Cosan Didem , Colak Ertugrul , Veysi Gunes Hasan , Degirmenci Irfan
Published: February 2018

The vitamin K epoxide reductase complex subunit 1 (VKORC1) gene is expressed in many tissue types, and encodes the VKORC1 protein, which is a key enzyme in the vitamin K cycle. Although researchers have focused on the effects of vitamin K on glucose metabolism, and on its role in the development of type 2 diabetes (T2DM), no consensus has yet been reached. Therefore, here we aimed to investigate the association between VKORC1 variants and the risk of T2DM. The 3673G / A (rs9923231) and 9041G / A (rs7294) VKORC1 variants were investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 100 control individuals and 100 patients with T2DM. The genomic regions were amplified by PCR; amplicons were digested using the AciI and NciI enzymes and visualized by agarose gel electrophoresis. The genotype frequencies of the 3673G / A variants were GG (22%), GA (56%), and AA (22%) in the control group and GG (19%), GA (52%), and AA (29%) in patients with T2DM (p > 0.05). The genotype frequencies of the 9041G / A variants were GG (37%), GA (53%), and AA (10%) in the control group and GG (46%), GA (45%), and AA (9%) in patients with T2DM (p > 0.05). In conclusion, we found no significant correlation between the control group and patients with T2DM, with regard to the different genetic models of the 3673G / A and 9041G / A variants. These data suggest that these VKORC1 gene variants may not be linked to T2DM.
Histone deacetylase inhibitor ITF2357 leads to apoptosis and enhances doxorubicin cytotoxicity in preclinical models of human sarcoma
Authors: Di Martile Marta , Desideri Marianna , Tupone Maria Grazia , Buglioni Simonetta , Antoniani Barbara , Mastroiorio Carlotta , Falcioni Rita , Ferraresi Virginia , Baldini Nicola , Biagini Roberto , Milella Michele , Trisciuoglio Daniela , Del Bufalo Donatella
Published: February 2018

Sarcomas are rare tumors with generally poor prognosis, for which current therapies have shown limited efficacy. Histone deacetylase inhibitors (HDACi) are emerging anti-tumor agents; however, little is known about their effect in sarcomas. By using established and patient-derived sarcoma cells with different subtypes, we showed that the pan-HDACi, ITF2357, potently inhibited in vitro survival in a p53-independent manner. ITF2357-mediated cell death implied the activation of mitochondrial apoptosis, as attested by induction of pro-apoptotic BH3-only proteins and a caspases-dependent mechanism. ITF2357 also induced autophagy, which protected sarcoma cells from apoptotic cell death. ITF2357 activated forkhead box (FOXO) 1 and 3a transcription factors and their downstream target genes, however, silencing of both FOXO1 and 3a did not protect sarcoma cells against ITF2357-induced apoptosis and upregulated FOXO4 and 6. Notably, ITF2357 synergized with Doxorubicin to induce cell death of established and patient-derived sarcoma cells. Furthermore, combination treatment strongly impaired xenograft tumor growth in vivo, when compared to single treatments, suggesting that combination of ITF2357 with Doxorubicin has the potential to enhance sensitization in different preclinical models of sarcoma. Overall, our study highlights the therapeutic potential of ITF2357, alone or in rational combination therapies, for bone and soft tissue sarcomas management.
Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development
Authors: Su Xiaomin , Wu Chenglei , Ye Xiaoying , Zeng Ming , Zhang Zhujun , Che Yongzhe , Zhang Yuan , Liu Lin , Lin Yushuang , Yang Rongcun
Published: February 2018

TRIM family members have been implicated in a variety of biological processes such as differentiation and development. We here found that Trim59 plays a critical role in early embryo development from blastocyst stage to gastrula. There existed delayed development and empty yolk sacs from embryonic day (E) 8.5 in Trim59−/− embryos. No viable Trim59−/− embryos were observed beyond E9.5. Trim59 deficiency affected primary germ layer formation at the beginning of gastrulation. At E6.5 and E7.5, the expression of primary germ layer formation-associated genes including Brachyury, lefty2, Cer1, Otx2, Wnt3, and BMP4 was reduced in Trim59−/− embryos. Homozygous mutant embryonic epiblasts were contracted and the mesoderm was absent. Trim59 could interact with actin- and myosin-associated proteins. Its deficiency disturbed F-actin polymerization during inner cell mass differentiation. Trim59-mediated polymerization of F-actin was via WASH K63-linked ubiquitination. Thus, Trim59 may be a critical regulator for early embryo development from blastocyst stage to gastrula through modulating F-actin assembly.
Estradiol signaling mediates gender difference in visceral adiposity via autophagy
Authors: Tao Zhipeng , Zheng Louise D. , Smith Cayleen , Luo Jing , Robinson Alex , Almeida Fabio A. , Wang Zongwei , Olumi Aria F. , Liu Dongmin , Cheng Zhiyong
Published: February 2018

Excessive adiposity (particularly visceral fat mass) increases the risks of developing metabolic syndrome. Women have lower deposit of visceral fat than men, and this pattern becomes diminished postmenopausally, but the underlying mechanism remains largely unknown. Here, we show that the gender difference in visceral fat distribution is controlled by an estradiol–autophagy axis. In C57BL/6J and wild-type control mice, a higher visceral fat mass was detected in the males than in the females, which was associated with lower expression of estrogen receptor α (ERα) and more active autophagy in males vs. females. However, deletion of ERα normalized autophagy activity and abolished the gender difference in visceral adiposity. In line with the adiposity-reducing effect of the ERα–autophagy axis, we found that downregulation of ERα and increased autophagy activity were required for adipogenesis, while induction of estradiol signaling dampened autophagy and drastically prevented adipogenesis. Mechanistically, the estradiol-ERα signaling activated mTOR, which phosphorylated and inhibited ULK1, thereby suppressing autophagy and adipogenesis. Together, our study suggests that the lower visceral adiposity in the females (vs. the males) arises from a more active estradiol-ERα signaling, which tunes down autophagy and adipogenesis.
Dual properties of a hydrogen oxidation Ni-catalyst entrapped within a polymer promote self-defense against oxygen
Authors: Oughli Alaa A. , Ruff Adrian , Boralugodage Nilusha Priyadarshani , Rodríguez-Maciá Patricia , Plumeré Nicolas , Lubitz Wolfgang , Shaw Wendy J. , Schuhmann Wolfgang , Rüdiger Olaf
Published: February 2018

The Ni(P2N2)2 catalysts are among the most efficient non-noble-metal based molecular catalysts for H2 cycling. However, these catalysts are O2 sensitive and lack long term stability under operating conditions. Here, we show that in a redox silent polymer matrix the catalyst is dispersed into two functionally different reaction layers. Close to the electrode surface is the “active” layer where the catalyst oxidizes H2 and exchanges electrons with the electrode generating a current. At the outer film boundary, insulation of the catalyst from the electrode forms a “protection” layer in which H2 is used by the catalyst to convert O2 to H2O, thereby providing the “active” layer with a barrier against O2. This simple but efficient polymer-based electrode design solves one of the biggest limitations of these otherwise very efficient catalysts enhancing its stability for catalytic H2 oxidation as well as O2 tolerance.
Heritable DNA methylation marks associated with susceptibility to breast cancer
Authors: Joo Jihoon E. , Dowty James G. , Milne Roger L. , Wong Ee Ming , Dugué Pierre-Antoine , English Dallas , Hopper John L. , Goldgar David E. , Giles Graham G. , Southey Melissa C. ,   kConFab, Sexton Adrienne , Christian Alice , Trainer Alison , Spigelman Allan , Fellows Andrew , Shelling Andrew , De Fazio Anna , Blackburn Anneke , Crook Ashley , Meiser Bettina , Patterson Briony , Clarke Christine , Saunders Christobel , Hunt Clare , Scott Clare , Amor David , Marsh Deborah , Edkins Edward , Salisbury Elizabeth , Haan Eric , Neidermayr Eveline , Macrae Finlay , Farshid Gelareh , Lindeman Geoff , Chenevix-Trench Georgia , Mann Graham , Gill Grantley , Thorne Heather , Campbell Ian , Hickie Ian , Winship Ingrid , Goldblatt Jack , Flanagan James , Kollias James , Visvader Jane , Stone Jennifer , Taylor Jessica , Burke Jo , Saunus Jodi , Forbes John , Beesley Jonathan , Kirk Judy , French Juliet , Tucker Kathy , Wu Kathy , Phillips Kelly , Lipton Lara , Andrews Leslie , Lobb Elizabeth , Walker Logan , Kentwell Maira , Spurdle Amanda , Cummings Margaret , Gleeson Margaret , Harris Marion , Jenkins Mark , Young Mary Anne , Delatycki Martin , Wallis Mathew , Burgess Matthew , Price Melanie , Brown Melissa , Bogwitz Michael , Field Michael , Friedlander Michael , Gattas Michael , Saleh Mona , Hayward Nick , Pachter Nick , Cohen Paul , Duijf Pascal , James Paul , Simpson Peter , Fong Peter , Butow Phyllis , Williams Rachael , Kefford Richard , Scott Rodney , Balleine Rosemary , Dawson Sarah-Jane , Lok Sheau , O’Connell Shona , Greening Sian , Nightingale Sophie , Edwards Stacey , Fox Stephen , McLachlan Sue-Anne , Lakhani Sunil , Thomas Susan , Antill Yoland
Published: February 2018

Mendelian-like inheritance of germline DNA methylation in cancer susceptibility genes has been previously reported. We aimed to scan the genome for heritable methylation marks associated with breast cancer susceptibility by studying 25 Australian multiple-case breast cancer families. Here we report genome-wide DNA methylation measured in 210 peripheral blood DNA samples provided by family members using the Infinium HumanMethylation450. We develop and apply a new statistical method to identify heritable methylation marks based on complex segregation analysis. We estimate carrier probabilities for the 1000 most heritable methylation marks based on family structure, and we use Cox proportional hazards survival analysis to identify 24 methylation marks with corresponding carrier probabilities significantly associated with breast cancer. We replicate an association with breast cancer risk for four of the 24 marks using an independent nested case–control study. Here, we report a novel approach for identifying heritable DNA methylation marks associated with breast cancer risk.
3D printing of robotic soft actuators with programmable bioinspired architectures
Authors: Schaffner Manuel , Faber Jakob A. , Pianegonda Lucas , Rühs Patrick A. , Coulter Fergal , Studart André R.
Published: February 2018

Soft actuation allows robots to interact safely with humans, other machines, and their surroundings. Full exploitation of the potential of soft actuators has, however, been hindered by the lack of simple manufacturing routes to generate multimaterial parts with intricate shapes and architectures. Here, we report a 3D printing platform for the seamless digital fabrication of pneumatic silicone actuators exhibiting programmable bioinspired architectures and motions. The actuators comprise an elastomeric body whose surface is decorated with reinforcing stripes at a well-defined lead angle. Similar to the fibrous architectures found in muscular hydrostats, the lead angle can be altered to achieve elongation, contraction, or twisting motions. Using a quantitative model based on lamination theory, we establish design principles for the digital fabrication of silicone-based soft actuators whose functional response is programmed within the material's properties and architecture. Exploring such programmability enables 3D printing of a broad range of soft morphing structures.
Monovalent manganese based anodes and co-solvent electrolyte for stable low-cost high-rate sodium-ion batteries
Authors: Firouzi Ali , Qiao Ruimin , Motallebi Shahrokh , Valencia Christian W. , Israel Hannah S. , Fujimoto Mai , Wray L. Andrew , Chuang Yi-De , Yang Wanli , Wessells Colin D.
Published: February 2018

The demand of sustainable power supply requires high-performance cost-effective energy storage technologies. Here we report a high-rate long-life low-cost sodium-ion battery full-cell system by innovating both the anode and the electrolyte. The redox couple of manganese(I/II) in Prussian blue analogs enables a high-rate and stable anode. Soft X-ray absorption spectroscopy and resonant inelastic X-ray scattering provide direct evidence suggesting the existence of monovalent manganese in the charged anode. There is a strong hybridization between cyano ligands and manganese-3d states, which benefits the electronic property for improving rate performance. Additionally, we employ an organic–aqueous cosolvent electrolyte to solve the long-standing solubility issue of Prussian blue analogs. A full-cell sodium-ion battery with low-cost Prussian blue analogs in both electrodes and co-solvent electrolyte retains 95% of its initial discharge capacity after 1000 cycles at 1C and 95% depth of discharge. The revealed manganese(I/II) redox couple inspires conceptual innovations of batteries based on atypical oxidation states.
PAN-cancer analysis of S-phase enriched lncRNAs identifies oncogenic drivers and biomarkers
Authors: Ali Mohamad Moustafa , Akhade Vijay Suresh , Kosalai Subazini Thankaswamy , Subhash Santhilal , Statello Luisa , Meryet-Figuiere Matthieu , Abrahamsson Jonas , Mondal Tanmoy , Kanduri Chandrasekhar
Published: February 2018

Despite improvement in our understanding of long noncoding RNAs (lncRNAs) role in cancer, efforts to find clinically relevant cancer-associated lncRNAs are still lacking. Here, using nascent RNA capture sequencing, we identify 1145 temporally expressed S-phase-enriched lncRNAs. Among these, 570 lncRNAs show significant differential expression in at least one tumor type across TCGA data sets. Systematic clinical investigation of 14 Pan-Cancer data sets identified 633 independent prognostic markers. Silencing of the top differentially expressed and clinically relevant S-phase-enriched lncRNAs in several cancer models affects crucial cancer cell hallmarks. Mechanistic investigations on SCAT7 in multiple cancer types reveal that it interacts with hnRNPK/YBX1 complex and affects cancer cell hallmarks through the regulation of FGF/FGFR and its downstream PI3K/AKT and MAPK pathways. We also implement a LNA-antisense oligo-based strategy to treat cancer cell line and patient-derived tumor (PDX) xenografts. Thus, this study provides a comprehensive list of lncRNA-based oncogenic drivers with potential prognostic value.
Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen
Authors: Stewart Robert D. , Auffret Marc D. , Warr Amanda , Wiser Andrew H. , Press Maximilian O. , Langford Kyle W. , Liachko Ivan , Snelling Timothy J. , Dewhurst Richard J. , Walker Alan W. , Roehe Rainer , Watson Mick
Published: February 2018

The cow rumen is adapted for the breakdown of plant material into energy and nutrients, a task largely performed by enzymes encoded by the rumen microbiome. Here we present 913 draft bacterial and archaeal genomes assembled from over 800 Gb of rumen metagenomic sequence data derived from 43 Scottish cattle, using both metagenomic binning and Hi-C-based proximity-guided assembly. Most of these genomes represent previously unsequenced strains and species. The draft genomes contain over 69,000 proteins predicted to be involved in carbohydrate metabolism, over 90% of which do not have a good match in public databases. Inclusion of the 913 genomes presented here improves metagenomic read classification by sevenfold against our own data, and by fivefold against other publicly available rumen datasets. Thus, our dataset substantially improves the coverage of rumen microbial genomes in the public databases and represents a valuable resource for biomass-degrading enzyme discovery and studies of the rumen microbiome.
Prediction of Postnatal Growth Failure among Very Low Birth Weight Infants
Authors: Lee Soon Min , Kim Namhyo , Namgung Ran , Park Minsoo , Park Kookin , Jeon Jihyun
Published: February 2018

Postnatal growth failure (PGF) in preterm infants remains an important clinical issue. In this study, we analysed the incidence of PGF among very low birth weight (VLBW) infants and evaluated the risk factors for PGF based on the data of 2799 VLBW infants obtained from the Korean Neonatal Network database from 2013 to 2014. PGF was defined as a decrease in weight Z score between birth and discharge of more than −1.28 using the Fenton growth charts. Risk factors were evaluated in relation to birth weight for gestational age, namely small (SGA) or appropriate (AGA) for gestational age, using propensity score matching used for between-group differences. The overall incidence of PGF was 45.5%, with a rate of 68.9% in the SGA group and 36.2% in the AGA group. PGF was negatively correlated with gestation and birth weight; additionally, PGF was associated with a higher incidence of co-morbidities. Predictors of PGF in the SGA group were respiratory distress syndrome and days to attain 100 mL/kg of enteral feeding. The only predictor of PGF in the AGA group was days to attain 100 mL/kg of enteral feeding. Early initiation and aggressive progression of enteral nutrition may decrease the incidence of PGF.
Nox4 is a Target for Tuberin Deficiency Syndrome
Authors: Shi Qian , Viswanadhapalli Suryavathi , Friedrichs William E. , Velagapudi Chakradhar , Szyndralewiez Cédric , Bansal Shweta , Bhat Manzoor A. , Choudhury Goutam Ghosh , Abboud Hanna E.
Published: February 2018

The mechanism by which TSC2 inactivation or deficiency contributes to the pathology of tuberous sclerosis complex (TSC) is not fully clear. We show that renal angiomyolipomas from TSC patients and kidney cortex from Tsc2+/− mice exhibit elevated levels of reactive oxygen species (ROS). Downregulation of tuberin (protein encoded by TSC2 gene) in renal proximal tubular epithelial cells significantly increased ROS concomitant with enhanced Nox4. Similarly, we found elevated levels of Nox4 in the renal cortex of Tsc2+/− mice and in the renal angiomyolipomas from TSC patients. Tuberin deficiency is associated with activation of mTORC1. Rapamycin, shRNAs targeting raptor, or inhibition of S6 kinase significantly inhibited the expression of Nox4, resulting in attenuation of production of ROS in tuberin-downregulated proximal tubular epithelial cells. In contrast, activation of mTORC1 increased Nox4 and ROS. These results indicate that Nox4 may be a potential target for tuberin-deficiency-derived diseases. Using a xenograft model from tuberin-null tubular cells in nude mice, both anti-sense Nox4 and GKT137831, a specific inhibitor of Nox1/4, significantly inhibited the tumor growth. Thus, our results demonstrate the presence of an antagonistic relationship between tuberin and Nox4 to drive oncogenesis in the tuberin deficiency syndrome and identify Nox4 as a target to develop a therapy for TSC.
Ablation of Bax and Bak protects skeletal muscle against pressure-induced injury
Authors: Tam Bjorn T. , Yu Angus P. , Tam Eric W. , Monks Douglas A. , Wang Xu P. , Pei Xiao M. , Koh Su P. , Sin Thomas K. , Law Helen K. W. , Ugwu Felix N. , Supriya Rashmi , Yung Benjamin Y. , Yip Shea P. , Wong S. C. , Chan Lawrence W. , Lai Christopher W. , Ouyang Pin , Siu Parco M.
Published: February 2018

Pressure-induced injury (PI), such as a pressure ulcer, in patients with limited mobility is a healthcare issue worldwide. PI is an injury to skin and its underlying tissue such as skeletal muscle. Muscle compression, composed of mechanical deformation of muscle and external load, leads to localized ischemia and subsequent unloading reperfusion and, hence, a pressure ulcer in bed-bound patients. Although the gross factors involved in PI have been identified, little is known about the exact disease mechanism or its links to apoptosis, autophagy and inflammation. Here, we report that PI is mediated by intrinsic apoptosis and exacerbated by autophagy. Conditional ablation of Bax and Bak activates the Akt-mTOR pathway and Bnip3-mediated mitophagy and preserves mitochondrial contents in compressed muscle. Moreover, we find that the presence/absence of Bax and Bak alters the roles and functions of autophagy in PI. Our results suggest that manipulating apoptosis and autophagy are potential therapeutic targets for treatment and prevention of PI.
FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis
Authors: Sambucci Manolo , Gargano Francesca , De Rosa Veronica , De Bardi Marco , Picozza Mario , Placido Roberta , Ruggieri Serena , Capone Alessia , Gasperini Claudio , Matarese Giuseppe , Battistini Luca , Borsellino Giovanna
Published: February 2018

Forkhead box P3 (FoxP3)+ regulatory T cells (Treg) are powerful mediators of immune regulation and immune homeostasis. In humans, Tregs are a heterogeneous population expressing surface markers which define phenotypically and functionally distinct subsets. Moreover, it is now clear that intracellular staining for FoxP3 does not unequivocally identify “true” suppressor cells, since several FoxP3 isoforms exist, and different reagents for FoxP3 detection are available. Here, we propose a strategy to identify potentially functional and suppressive Treg cells in an autoimmune disease like multiple sclerosis, and we suggest that in patients affected by this disease these cells are both reduced in number and functionally exhausted.
Anisotropic N-Graphene-diffused Co3O4 nanocrystals with dense upper-zone top-on-plane exposure facets as effective ORR electrocatalysts
Authors: Hassen D. , Shenashen M. A. , El-Safty A. R. , Elmarakbi A. , El-Safty S. A.
Published: February 2018

We provide strong evidence of the effectiveness of homogenously self-propelled particle-in-particle diffusion, interaction and growth protocol. This technique was used for one-pot synthesis of novel nitrogen-graphene oxide (N-GO)/Co3O4 nanocrystals with cuboid rectangular prism-shaped nanorods (NRs) along {110}-plane and truncated polyhedrons with densely-exposed, multi-facet sites along {311} and {111} planes. These hierarchal nanocrystals create electrode catalyst patterns with vast-range accessibility to active Co2+ sites, a vascular system for the transport and retention of captured O2 molecule interiorly, and low adsorption energy and dense electron configuration surfaces during the oxygen reduction reaction (ORR). The superior electrocatalytic ORR activity of the N-GO/Co3O4 polyhedron nanocrystals in terms of electrochemical selectivity, durability and stability compared with NRs or commercial Pt/C catalysts confirms the synergetic contribution of multi-functional, dense-exposed, and actively topographic facets of polyhedrons to significantly activate the catalytic nature of the catalyst. Our findings show real evidence, for the first time that not only the large number of catalytically active Co2+ cations at the top surface layer but also the dense location of active Co2+ sites on the upper-zone top-on-plane exposure, and the electron density configuration and distribution around the Co2+ sites were important for effective ORR.
Quantitative account of social interactions in a mental health care ecosystem: cooperation, trust and collective action
Authors: Cigarini Anna , Vicens Julián , Duch Jordi , Sánchez Angel , Perelló Josep
Published: February 2018

Mental disorders have an enormous impact in our society, both in personal terms and in the economic costs associated with their treatment. In order to scale up services and bring down costs, administrations are starting to promote social interactions as key to care provision. We analyze quantitatively the importance of communities for effective mental health care, considering all community members involved. By means of citizen science practices, we have designed a suite of games that allow to probe into different behavioral traits of the role groups of the ecosystem. The evidence reinforces the idea of community social capital, with caregivers and professionals playing a leading role. Yet, the cost of collective action is mainly supported by individuals with a mental condition - which unveils their vulnerability. The results are in general agreement with previous findings but, since we broaden the perspective of previous studies, we are also able to find marked differences in the social behavior of certain groups of mental disorders. We finally point to the conditions under which cooperation among members of the ecosystem is better sustained, suggesting how virtuous cycles of inclusion and participation can be promoted in a ‘care in the community’ framework.
Varying temperature and silicon content in nanodiamond growth: effects on silicon-vacancy centres
Authors: Choi Sumin , Leong Victor , Davydov Valery A. , Agafonov Viatcheslav N. , Cheong Marcus W. O. , Kalashnikov Dmitry A. , Krivitsky Leonid A.
Published: February 2018

Nanodidamonds containing colour centres open up many applications in quantum information processing, metrology, and quantum sensing. However, controlling the synthesis of nanodiamonds containing silicon vacancy (SiV) centres is still not well understood. Here we study nanodiamonds produced by a high-pressure high-temperature method without catalyst metals, focusing on two samples with clear SiV signatures. Different growth temperatures and relative content of silicon in the initial compound between the samples altered their nanodiamond size distributions and abundance of SiV centres. Our results show that nanodiamond growth can be controlled and optimised for different applications.
Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H
Authors: Ito Fumiaki , Yang Hanjing , Xiao Xiao , Li Shu-Xing , Wolfe Aaron , Zirkle Brett , Arutiunian Vagan , Chen Xiaojiang S.
Published: February 2018

APOBEC3H (A3H) is a member of the APOBEC3 subfamily of DNA cytosine deaminases that are important for innate immune defense and have been implicated in cancer biogenesis. To understand the structural basis for A3H biochemical function, we determined a high-resolution structure of human A3H and performed extensive biochemical analysis. The 2.49 Å crystal structure reveals a uniquely long C-terminal helix 6 (h6), a disrupted β5 strand of the canonical five-stranded β-sheet core, and a long loop 1 around the Zn-active center. Mutation of a loop 7 residue, W115, disrupted the RNA-mediated dimerization of A3H yielding an RNA-free monomeric form that still possessed nucleic acid binding and deaminase activity. A3H expressed in HEK293T cells showed RNA dependent HMW complex formation and RNase A-dependent deaminase activity. A3H has a highly positively charged surface surrounding the Zn-active center, and multiple positively charged residues within this charged surface play an important role in the RNA-mediated HMW formation and deaminase inhibition. Furthermore, these positively charged residues affect subcellular localization of A3H between the nucleus and cytosol. Finally, we have identified multiple residues of loop 1 and 7 that contribute to the overall deaminase activity and the methylcytosine selectivity.
Environmental factors driving fungal distribution in freshwater lake sediments across the Headwater Region of the Yellow River, China
Authors: Tian Jianqing , Zhu Dan , Wang Jinzhi , Wu Bing , Hussain Muzammil , Liu Xingzhong
Published: February 2018

Dispersal limitation and environmental filtering are two primary processes involved in shaping microbial community structure. The pristine environmental and geographical relatively isolation of small lakes distributed in the Headwater Region of Yellow River (HRYR) offer a unique opportunity to test the relative roles of these two processes on fungal communities. Here, we investigated the fungal community in sediment samples from 10 lakes located in the HRYR using high-throughput sequencing. The results showed that the fungal community was dominated by Sordariomycetes, Leotiomycetes, Dothideomycetes, Pezizomycetes and Agaricomycetes. The results revealed that altitude, mean annual temperature, C/N ration, dissolve organic carbon and total nitrogen were the best predictors for shaping fungal community structure in these lakes. Significant spatial and environmental distance decay relationships in the fungal community were detected. The partial Mantel test indicated that the fungal community structure was significantly correlated with environmental distance but not with geographic distance. Overall, environmental filtering plays a more important role than dispersal limitation in fungal community structure at a local scale in such an pristine and isolated region.
Body size influences energetic and osmoregulatory costs in frogs infected with Batrachochytrium dendrobatidis
Authors: Wu Nicholas C. , Cramp Rebecca L. , Franklin Craig E.
Published: February 2018

Sloughing maintains the skins integrity and critical functionality in amphibians. Given the behavioural, morphological and osmoregulatory changes that accompany sloughing, this process is likely to be physiologically costly. Chytridiomycosis, a cutaneous disease of amphibians caused by the fungus Batrachochytrium dendrobatidis (Bd), disrupts skin function and increases sloughing rates. Moreover, mortality rates from chytridiomycosis are significantly higher in juveniles and so we hypothesised that smaller individuals maybe more susceptible to chytridiomycosis because of allometric scaling effects on the energetic and osmoregulatory costs of sloughing. We measured in-vivo cutaneous ion loss rates and whole animal metabolic rate (MR) of Green tree frogs, Litoria caerulea, over a range of body sizes both infected and uninfected frogs during sloughing. Infected animals had a greater rate of ion loss and mass-specific MR during non-sloughing periods but there were no additional effects of sloughing on either of these parameters. There were also significant interactions with body size and Bd load indicating that smaller animals with higher Bd loads have greater rates of ion loss and higher energetic demands. Our results shed light on why smaller Bd-infected anurans often exhibit greater physiological disruption than larger individuals.