bluuebunny/crossref_metadata_2025 huggingface dataset:
Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers
Authors: Wang Weiwei , Li Zhui , Han Qiqi , Guo Yuming , Zhang Bo , D’inca Romain
Published: December 2016

The effects of live yeast (LY) and mannan-oligosaccharide (MOS) supplementation on intestinal disruption induced by Escherichia coli in broilers were investigated. The experimental design was a 3×2 factorial arrangement with three dietary treatments (control, 0·5 g/kg LY (Saccharomyces cerevisiae, 1·0×1010 colony-forming units/g), 0·5 g/kg MOS) and two immune treatments (with or without E. coli challenge from 7 to 11 d of age). Samples were collected at 14 d of age. The results showed that E. coli challenge impaired (P<0·05) growth performance during the grower period (1–21 d) and the overall period (1–35 d) of broilers, increased (P<0·05) serum endotoxin and diamine oxidase levels coupled with ileal myeloperoxidase and lysozyme activities, whereas reduced (P<0·05) maltase activity, and compromised the morphological structure of the ileum. Besides, it increased (P<0·05) the mRNA expressions of several inflammatory genes and reduced occludin expression in the ileum. Dietary treatment with both LY and MOS reduced (P<0·05) serum diamine oxidase and ileal myeloperoxidase levels, but elevated villus height (P<0·10) and the ratio of villus height:crypt depth (P<0·05) of the ileum. It also alleviated (P<0·05) E. coli-induced increases (P<0·05) in ileal Toll-like receptor 4, NF-κB and IL-1β expressions. Moreover, LY supplementation reduced (P<0·05) feed conversion ratio of birds during the grower period and enhanced (P<0·05) the community diversity (Shannon and Simpson indices) of ileal microbiota, whereas MOS addition counteracted (P<0·05) the decreased ileal IL-10 and occludin expressions in challenged birds. In conclusion, both LY and MOS supplementation could attenuate E. coli-induced intestinal disruption by alleviating intestinal inflammation and barrier dysfunction in broilers. Moreover, LY addition could improve intestinal microbial community structure and feed efficiency of broilers.
Effect of surgical intervention on middle-ear cholesteatoma with associated facial paralysis
Authors: Ozkul Y , Songu M , Onal K , Imre A , Arslanoglu S , Horoz E , Bayrak F , Pinar E
Published: December 2016

Objectives:

To investigate the presenting symptoms, intra-operative findings and long-term facial nerve function in patients treated for cholesteatoma with associated facial paralysis.

Methods:

Fifteen patients with facial paralysis due to middle-ear cholesteatoma who underwent tympanomastoidectomy surgery from February 2000 to February 2015 were retrospectively reviewed. After removal of the cholesteatoma, a limited area of the fallopian canal, in which facial nerve oedema or redness was evident, was opened. Incision of the epineural sheath for nerve decompression was not performed.

Results:

Pre-operative House–Brackmann grade was grade II in two patients, grade III in four, grade IV in seven, grade V in one and grade VI in one. Facial nerve perineurium damage was observed in two patients with poor prognoses. All patients treated within the first 15 days after paralysis onset showed normal facial function at long-term follow up. Post-operative House–Brackmann grade was grade I in 11 patients, grade II in 1, grade III in 2 and grade VI in 1.

Conclusion:

Early surgical treatment is more likely to give good results, and poor outcomes are observed in patients with facial nerve perineurium damage.
Practical fertilization procedure and embryonic development of the New Zealand geoduck clam (Panopea zelandica)
Authors: Le Dung V. , Young Tim , Alfaro Andrea C. , Ragg Norman L.C. , Hilton Zoë , Watts Ellie , King Nick
Published: December 2016

Cultivation of the geoduck Panopea zelandica (Quoy & Gaimard, 1835) requires knowledge on embryonic development to produce spat in hatcheries. This study investigated the development of P. zelandica embryos at 15°C and 35 ppt and the optimal sperm:egg ratios for fertilization under hatchery conditions. Panopea zelandica broodstock were induced to spawn by serotonin injection. Sperm and eggs were collected and fertilization was conducted at sperm:egg ratios of: 50:1, 100:1, 500:1, 1000:1 and 10,000:1 over 40 min. The optimal sperm:egg ratio was <500:1 and the normal embryo yield at 3 and 18 h post-fertilization (hpf) ranged from 83–96%. Panopea zelandica eggs (~80 μm diameter) developed the first and second polar bodies within 15–20 and 50–55 min post-fertilization, respectively. The blastula appeared at ~8 hpf, including the XR and XL cells and the presumptive shell field depression. Gastrulation occurred at 12–18 hpf with organic material apparent at the shell field depression. The mid-stage trochophore, which appeared at around 35 hpf had an apical plate with an apical tuft. The shell field spread to form the periostracum, which expanded and folded into right and left segments covering the late trochophore. The early D-stage veliger appeared at 45 hpf with the soft body being enclosed by two valves and the appearance of the velum. These observations will serve as the basis for future analyses of P. zelandica embryogenesis and for optimization of commercial production of D-veliger larvae.
EXACT LIKELIHOOD INFERENCE IN GROUP INTERACTION NETWORK MODELS
Authors: Hillier Grant , Martellosio Federico
Published: December 2016

The paper studies spatial autoregressive models with group interaction structure, focussing on estimation and inference for the spatial autoregressive parameterλ. The quasi-maximum likelihood estimator forλusually cannot be written in closed form, but using an exact result obtained earlier by the authors for its distribution function, we are able to provide a complete analysis of the properties of the estimator, and exact inference that can be based on it, in models that are balanced. This is presented first for the so-called pure model, with no regression component, but is also extended to some special cases of the more general model. We then study the much more difficult case of unbalanced models, giving analogues of some, but by no means all, of the results obtained for the balanced case earlier. In both balanced and unbalanced models, results obtained for the pure model generalize immediately to the model with group-specific regression components.
TWO NEW PERUVIAN SPECIES OF BEGONIA (BEGONIACEAE) AND AN AMENDED DESCRIPTION OF BEGONIA THYRSOIDEA
Authors: Moonlight P. W. , Tebbitt M. C.
Published: December 2016

Begonia speculum is described and illustrated as a new species endemic to the San Martín Region of Peru, and compared with Begonia cremnophila Tebbitt and Begonia lutea L.B.Sm. & B.G.Schub. Begonia erythrothrix is described and illustrated as a new species endemic to the Cuzco Region of Peru, and compared with an updated description of Begonia thyrsoidea Irmsch., which is lectotypified. Using International Union for Conservation of Nature criteria, Begonia speculum is considered Vulnerable; B. erythrothrix and B. thyrsoidea are assessed as Data Deficient.
Task shifting to clinical officer-led echocardiography screening for detecting rheumatic heart disease in Malawi, Africa
Authors: Sims Sanyahumbi Amy , Sable Craig A. , Karlsten Melissa , Hosseinipour Mina C. , Kazembe Peter N. , Minard Charles G. , Penny Daniel J.
Published: December 2016

Background

Echocardiographic screening for rheumatic heart disease in asymptomatic children may result in early diagnosis and prevent progression. Physician-led screening is not feasible in Malawi. Task shifting to mid-level providers such as clinical officers may enable more widespread screening.

Hypothesis

With short-course training, clinical officers can accurately screen for rheumatic heart disease using focussed echocardiography.

Methods

A total of eight clinical officers completed three half-days of didactics and 2 days of hands-on echocardiography training. Clinical officers were evaluated by performing screening echocardiograms on 20 children with known rheumatic heart disease status. They indicated whether children should be referred for follow-up. Referral was indicated if mitral regurgitation measured more than 1.5 cm or there was any measurable aortic regurgitation. The κ statistic was calculated to measure referral agreement with a paediatric cardiologist. Sensitivity and specificity were estimated using a generalised linear mixed model, and were calculated on the basis of World Heart Federation diagnostic criteria.

Results

The mean κ statistic comparing clinical officer referrals with the paediatric cardiologist was 0.72 (95% confidence interval: 0.62, 0.82). The κ value ranged from a minimum of 0.57 to a maximum of 0.90. For rheumatic heart disease diagnosis, sensitivity was 0.91 (95% confidence interval: 0.86, 0.95) and specificity was 0.65 (95% confidence interval: 0.57, 0.72).

Conclusion

There was substantial agreement between clinical officers and paediatric cardiologists on whether to refer. Clinical officers had a high sensitivity in detecting rheumatic heart disease. With short-course training, clinical officer-led echo screening for rheumatic heart disease is a viable alternative to physician-led screening in resource-limited settings.
High-dose adenosine for refractory supraventricular tachycardia: a case report and literature review
Authors: Dadi Gal , Fink Daniel , Weiser Giora
Published: December 2016

Supraventricular tachycardia is the most common significant arrhythmia in children. If prolonged, it may cause heart failure and progress to cardiogenic shock warranting prompt treatment. The recommended interventions following vagal manoeuvres are intravenous adenosine and in the unstable patient electrical cardioversion. We present an infant with an unstable supraventricular tachycardia that was resistant to electrical cardioversion and recommended doses of adenosine. He reverted to sinus rhythm with a higher dose of adenosine, suggesting that such doses may be required in refractory supraventricular tachycardia.
Buying less and wasting less food. Changes in household food energy purchases, energy intakes and energy density between 2007 and 2012 with and without adjustment for food waste
Authors: Whybrow Stephen , Horgan Graham W , Macdiarmid Jennie I
Published: December 2016

Objective

Consumers in the UK responded to the rapid increases in food prices between 2007 and 2009 partly by reducing the amount of food energy bought. Household food and drink waste has also decreased since 2007. The present study explored the combined effects of reductions in food purchases and waste on estimated food energy intakes and dietary energy density.

Design

The amount of food energy purchased per adult equivalent was calculated from Kantar Worldpanel household food and drink purchase data for 2007 and 2012. Food energy intakes were estimated by adjusting purchase data for food and drink waste, using waste factors specific to the two years and scaled for household size.

Setting

Scotland.

Subjects

Households in Scotland (n 2657 in 2007; n 2841 in 2012).

Results

The amount of food energy purchased decreased between 2007 and 2012, from 8·6 to 8·2 MJ/adult equivalent per d (P<0·001). After accounting for the decrease in food waste, estimated food energy intake was not significantly different (7·3 and 7·2 MJ/adult equivalent per d for 2007 and 2012, respectively; P=0·186). Energy density of foods purchased increased slightly from 700 to 706 kJ/100 g (P=0·010).

Conclusions

While consumers in Scotland reduced the amount of food energy that they purchased between 2007 and 2012, this was balanced by reductions in household food and drink waste over the same time, resulting in no significant change in net estimated energy intake of foods brought into the home.
JOB AUTHORITY AND EMERGING RACIAL INCOME INEQUALITIES IN THE PUBLIC SECTOR
Authors: Wilson George , Roscigno Vincent J.
Published: December 2016

Has the adoption of “new governance” reforms over the last two decades eroded the public sector as a long-standing occupational niche for African Americans? Utilizing data from the General Social Survey, we address this issue in the context of earnings “returns” to three levels of job authority for African American men and women relative to their White counterparts. Findings, derived from analyses of three waves of the General Social Survey, indicate that the acceleration of this “business model” of work organization in the public sector has had relatively profound and negative consequences for African American income. Specifically, racial parity in earnings returns at all levels of authority in the “pre-reform” period (1992–1994) progressively eroded during “early reform” (2000–2002) and then even more so during the “late reform” (2010–2012) period. Much of this growing public sector disadvantage—a disadvantage that is approaching that seen in the private sector—is driven largely by income gaps between White and African American men, although a similar (though smaller) racial gap is witnessed among women. We conclude by discussing the occupational niche status of public sector work for African Americans, calling for further analyses of the growing inequality patterns identified in our analyses, and drawing attention to the implications for contemporary racial disadvantages.
“IT’S LIKE WE HAVE AN ‘IN’ ALREADY”
Authors: Waring Chandra D. L.
Published: December 2016

The increasing bi/multiracial1community in the United States has generated much literature about racial identity and social psychological well-being. Drawing on sixty in-depth interviews with Black/White biracial Americans, this paper shifts the theoretical focus from identity and well-being to the conceptual development of how race shapes bi/multiracial Americans’ social interactions with both Whites and Blacks. The majority of participants reported interacting differently when in predominately White settings versus predominately Black settings. I offer the concept of “racial capital” to highlight the repertoire of racial resources (knowledge, experiences, meaning, and language) that biracial Americans use to negotiate racial boundaries in a highly racialized society. These findings reveal the continuing significance of racial boundaries in a population that is often celebrated as evidence of racial harmony in the United States.
Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
Authors: Deschout Hendrik , Lukes Tomas , Sharipov Azat , Szlag Daniel , Feletti Lely , Vandenberg Wim , Dedecker Peter , Hofkens Johan , Leutenegger Marcel , Lasser Theo , Radenovic Aleksandra
Published: December 2016

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed-cell focal adhesion images, we investigate the complementarity between PALM and SOFI in terms of spatial and temporal resolution. This PALM-SOFI framework is used to image focal adhesions in living cells, while obtaining a temporal resolution below 10 s. We visualize the dynamics of focal adhesions, and reveal local mean velocities around 190 nm min−1. The complementarity of PALM and SOFI is assessed in detail with a methodology that integrates a resolution and signal-to-noise metric. This PALM and SOFI concept provides an enlarged quantitative imaging framework, allowing unprecedented functional exploration of focal adhesions through the estimation of molecular parameters such as fluorophore densities and photoactivation or photoswitching kinetics.
A neuronal network of mitochondrial dynamics regulates metastasis
Authors: Caino M. Cecilia , Seo Jae Ho , Aguinaldo Angeline , Wait Eric , Bryant Kelly G. , Kossenkov Andrew V. , Hayden James E. , Vaira Valentina , Morotti Annamaria , Ferrero Stefano , Bosari Silvano , Gabrilovich Dmitry I. , Languino Lucia R. , Cohen Andrew R. , Altieri Dario C.
Published: December 2016

The role of mitochondria in cancer is controversial. Using a genome-wide shRNA screen, we now show that tumours reprogram a network of mitochondrial dynamics operative in neurons, including syntaphilin (SNPH), kinesin KIF5B and GTPase Miro1/2 to localize mitochondria to the cortical cytoskeleton and power the membrane machinery of cell movements. When expressed in tumours, SNPH inhibits the speed and distance travelled by individual mitochondria, suppresses organelle dynamics, and blocks chemotaxis and metastasis, in vivo. Tumour progression in humans is associated with downregulation or loss of SNPH, which correlates with shortened patient survival, increased mitochondrial trafficking to the cortical cytoskeleton, greater membrane dynamics and heightened cell invasion. Therefore, a SNPH network regulates metastatic competence and may provide a therapeutic target in cancer.
The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons
Authors: Ha Go Eun , Lee Jaekwang , Kwak Hankyul , Song Kiyeong , Kwon Jea , Jung Soon-Young , Hong Joohyeon , Chang Gyeong-Eon , Hwang Eun Mi , Shin Hee-Sup , Lee C. Justin , Cheong Eunji
Published: December 2016

Neuronal firing patterns, which are crucial for determining the nature of encoded information, have been widely studied; however, the molecular identity and cellular mechanisms of spike-frequency adaptation are still not fully understood. Here we show that spike-frequency adaptation in thalamocortical (TC) neurons is mediated by the Ca2+-activated Cl− channel (CACC) anoctamin-2 (ANO2). Knockdown of ANO2 in TC neurons results in significantly reduced spike-frequency adaptation along with increased tonic spiking. Moreover, thalamus-specific knockdown of ANO2 increases visceral pain responses. These results indicate that ANO2 contributes to reductions in spike generation in highly activated TC neurons and thereby restricts persistent information transmission.
Sirt1 regulates glial progenitor proliferation and regeneration in white matter after neonatal brain injury
Authors: Jablonska Beata , Gierdalski Marcin , Chew Li-Jin , Hawley Teresa , Catron Mackenzie , Lichauco Arturo , Cabrera-Luque Juan , Yuen Tracy , Rowitch David , Gallo Vittorio
Published: December 2016

Regenerative processes in brain pathologies require the production of distinct neural cell populations from endogenous progenitor cells. We have previously demonstrated that oligodendrocyte progenitor cell (OPC) proliferation is crucial for oligodendrocyte (OL) regeneration in a mouse model of neonatal hypoxia (HX) that reproduces diffuse white matter injury (DWMI) of premature infants. Here we identify the histone deacetylase Sirt1 as a Cdk2 regulator in OPC proliferation and response to HX. HX enhances Sirt1 and Sirt1/Cdk2 complex formation through HIF1α activation. Sirt1 deacetylates retinoblastoma (Rb) in the Rb/E2F1 complex, leading to dissociation of E2F1 and enhanced OPC proliferation. Sirt1 knockdown in culture and its targeted ablation in vivo suppresses basal and HX-induced OPC proliferation. Inhibition of Sirt1 also promotes OPC differentiation after HX. Our results indicate that Sirt1 is an essential regulator of OPC proliferation and OL regeneration after neonatal brain injury. Therefore, enhancing Sirt1 activity may promote OL recovery after DWMI.
The HIF/PHF8/AR axis promotes prostate cancer progression
Authors: Tong D , Liu Q , Liu G , Yuan W , Wang L , Guo Y , Lan W , Zhang D , Dong S , Wang Y , Xiao H , Mu J , Mao C , Wong J , Jiang J
Published: December 2016

Recent studies provide strong evidence that the androgen receptor (AR) signaling pathway remains active in castration-resistant prostate cancer (CRPC). However, the underlying mechanisms are not well understood. In this study, we demonstrate that plant homeo domain finger protein 8 (PHF8 )interacts with and functions as an essential histone demethylase activity-dependent AR coactivator. Furthermore, we demonstrate that the expression of PHF8 is induced by hypoxia in various prostate cancer cell lines. Knockdown of either hypoxia-inducible factor HIF2α or HIF1α almost completely abolished hypoxia-induced PHF8 expression. Importantly, we observed that PHF8 is highly expressed in clinical androgen deprived prostate cancer samples and expression of PHF8 correlates with increased levels of HIF1α and HIF2α. Moreover, elevated PHF8 is associated with higher grade prostate cancers and unfavorable outcomes. Our findings support a working model in which hypoxia in castrated prostate cancer activates HIF transcription factors which then induces PHF8 expression. The elevated PHF8 in turn promotes the AR signaling pathway and prostate cancer progression. Therefore, the HIF/PHF8/AR axis could serve as a potential biomarker for CRPC and is also a promising therapeutic target in combating CRPC.
Lower and upper bounds for entanglement of Rényi-α entropy
Authors: Song Wei , Chen Lin , Cao Zhuo-Liang
Published: December 2016

Entanglement Rényi-α entropy is an entanglement measure. It reduces to the standard entanglement of formation when α tends to 1. We derive analytical lower and upper bounds for the entanglement Rényi-α entropy of arbitrary dimensional bipartite quantum systems. We also demonstrate the application our bound for some concrete examples. Moreover, we establish the relation between entanglement Rényi-α entropy and some other entanglement measures.
The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation
Authors: Mahale Sagar , Kumar Megha , Sharma Amit , Babu Aswini , Ranjan Shashi , Sachidanandan Chetana , Mylavarapu Sivaram V. S.
Published: December 2016

Cytoplasmic dynein 1 is a multi-protein intracellular motor essential for mediating several mitotic functions, including the establishment of proper spindle orientation. The functional relevance and mechanistic distinctions between two discrete dynein subpopulations distinguished only by Light Intermediate Chain (LIC) homologues, LIC1 and LIC2 is unknown during mitosis. Here, we identify LIC2-dynein as the major mediator of proper spindle orientation and uncover its underlying molecular mechanism. Cortically localized dynein, essential for maintaining correct spindle orientation, consists majorly of LIC2-dynein, which interacts with cortical 14-3-3 ε- ζ and Par3, conserved proteins required for orienting the spindle. LIC2-dynein is also responsible for the majority of dynein-mediated asymmetric poleward transport of NuMA, helping focus microtubule minus ends. In addition, LIC2-dynein dominates in equatorially aligning chromosomes at metaphase and in regulating mitotic spindle length. Key mitotic functions of LIC2 were remarkably conserved in and essential for early embryonic divisions and development in zebrafish. Thus LIC2-dynein exclusively engages with two major cortical pathways to govern spindle orientation. Overall, we identify a novel selectivity of molecular interactions between the two LICs in mitosis as the underlying basis for their uneven distribution of labour in ensuring proper spindle orientation.
Genome resequencing and comparative variome analysis in a Brassica rapa and Brassica oleracea collection
Authors: Cheng Feng , Wu Jian , Cai Chengcheng , Fu Lixia , Liang Jianli , Borm Theo , Zhuang Mu , Zhang Yangyong , Zhang Fenglan , Bonnema Guusje , Wang Xiaowu
Published: December 2016

The closely related species Brassica rapa and B. oleracea encompass a wide range of vegetable, fodder and oil crops. The release of their reference genomes has facilitated resequencing collections of B. rapa and B. oleracea aiming to build their variome datasets. These data can be used to investigate the evolutionary relationships between and within the different species and the domestication of the crops, hereafter named morphotypes. These data can also be used in genetic studies aiming at the identification of genes that influence agronomic traits. We selected and resequenced 199 B. rapa and 119 B. oleracea accessions representing 12 and nine morphotypes, respectively. Based on these resequencing data, we obtained 2,249,473 and 3,852,169 high quality SNPs (single-nucleotide polymorphisms), as well as 303,617 and 417,004 InDels for the B. rapa and B. oleracea populations, respectively. The variome datasets of B. rapa and B. oleracea represent valuable resources to researchers working on evolution, domestication or breeding of Brassica vegetable crops.
An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process
Authors: Mesnage Robin , Agapito-Tenfen Sarah Z. , Vilperte Vinicius , Renney George , Ward Malcolm , Séralini Gilles-Eric , Nodari Rubens O. , Antoniou Michael N.
Published: December 2016

Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as ‘substantially equivalent’ to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.
Fabrication of Si negative electrodes for Li-ion batteries (LIBs) using cross-linked polymer binders
Authors: Jang Suk-Yong , Han Sien-Ho
Published: December 2016

Currently, Si as an active material for LIBs has been attracting much attention due to its high theoretical specific capacity (3572 mAh g−1). However, a disadvantage when using a Si negative electrode for LIBs is the abrupt drop of its capabilities during the cycling process. Therefore, there have been a few studies of polymers such as poly(vinylidene fluoride) (PVdF), carboxymethyl cellulose (CMC), styrene butadiene rubber (SBR) and polyacrylic acid (PAA) given that the robust structure of a polymeric binder to LIBs anodes is a promising means by which to enhance the performance of high-capacity anodes. These studies essentially focused mainly on modifying of the linear-polymer component or on copolymers dissolved in solvents. Cross-linking polymers as a binder may be preferred due to their good scratch resistance, excellent chemical resistance and high levels of adhesion and resilience. However, because these types of polymers (with a rigid structure and cross-linking points) are also insoluble in general organic solvents, applying these types in this capacity is virtually impossible.
Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes
Authors: Yi Zi-Yun , Ma Xue-Shan , Liang Qiu-Xia , Zhang Teng , Xu Zhao-Yang , Meng Tie-Gang , Ouyang Ying-Chun , Hou Yi , Schatten Heide , Sun Qing-Yuan , Quan Song
Published: December 2016

Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation.
Sub-cycle ionization dynamics revealed by trajectory resolved, elliptically-driven high-order harmonic generation
Authors: Larsen E. W. , Carlström S. , Lorek E. , Heyl C. M. , Paleček D. , Schafer K. J. , L’Huillier A. , Zigmantas D. , Mauritsson J.
Published: December 2016

The sub-cycle dynamics of electrons driven by strong laser fields is central to the emerging field of attosecond science. We demonstrate how the dynamics can be probed through high-order harmonic generation, where different trajectories leading to the same harmonic order are initiated at different times, thereby probing different field strengths. We find large differences between the trajectories with respect to both their sensitivity to driving field ellipticity and resonant enhancement. To accurately describe the ellipticity dependence of the long trajectory harmonics we must include a sub-cycle change of the initial velocity distribution of the electron and its excursion time. The resonant enhancement is observed only for the long trajectory contribution of a particular harmonic when a window resonance in argon, which is off-resonant in the field-free case, is shifted into resonance due to a large dynamic Stark shift.
The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus
Authors: Wang Kang , Xie Chun , Zhang Jianan , Zhang Wenchao , Yang Deqiang , Yu Lingxue , Jiang Yifeng , Yang Shen , Gao Fei , Yang Zhibiao , Zhou Yanjun , Tong Guangzhi
Published: December 2016

Porcine epidemic diarrhea virus (PEDV) is a highly contagiouscoronavirusthat causes severe diarrhea and death, particularly in neonatal piglets. The nucleocapsid protein (N protein) of PEDV presents strong immunogenicity and contributes to the cross-reactivity between PEDV and TGEV. However, the characterization of epitopes on the PEDV N protein remains largely unknown. Here, two monoclonal antibodies (MAbs) specific to the N protein of a PEDV strain, FJzz1/2011, were generated and screened against a partially overlapping library of 24 GST-fusion N protein-truncated constructs. We confirmed that residues 18–133 (designated NEP-D4) and residues 252–262 (designated NEP-D6) were the epitopes targeted by MAbs PN-D4 and PN-D6, respectively. Sequence analysis revealed that these two epitopes were highly conserved among PEDV strains but were significantly different from other members of theCoronavirinaesubfamily. Western blot analysis showed that they could be specifically recognized by PEDV antisera but could not be recognized by TGEV hyperimmune antisera. Indirect immunofluorescence (IFA) assays confirmed no cross-reaction between these two MAbs and TGEV. In addition, the freeze-thaw cycle and protease treatment results indicated that NEP-D4 was intrinsically disordered. All these results suggest that these two novel epitopes and their cognate MAbs could serve as the basis for the development of precise diagnostic assays for PEDV.
Distinct urine metabolome after Asian ginseng and American ginseng intervention based on GC-MS metabolomics approach
Authors: Yang Liu , Yu Qing-Tao , Ge Ya-Zhong , Zhang Wen-Song , Fan Yong , Ma Chung-Wah , Liu Qun , Qi Lian-Wen
Published: December 2016

Ginseng occupies a prominent position in the list of best-selling natural products worldwide. Asian ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) show different properties and medicinal applications in pharmacology, even though the main active constituents of them are both thought to be ginsenosides. Metabolomics is a promising method to profile entire endogenous metabolites and monitor their fluctuations related to exogenous stimulus. Herein, an untargeted metabolomics approach was applied to study the overall urine metabolic differences between Asian ginseng and American ginseng in mice. Metabolomics analyses were performed using gas chromatography-mass spectrometry (GC-MS) together with multivariate statistical data analysis. A total of 21 metabolites related to D-glutamine and D-glutamate metabolism, glutathione metabolism, TCA cycle and glyoxylate and dicarboxylate metabolism, differed significantly under the Asian ginseng treatment; 34 metabolites mainly associated with glyoxylate and dicarboxylate metabolism, TCA cycle and taurine and hypotaurine metabolism, were significantly altered after American ginseng treatment. Urinary metabolomics reveal that Asian ginseng and American ginseng can benefit organism physiological and biological functions via regulating multiple metabolic pathways. The important pathways identified from Asian ginseng and American ginseng can also help to explore new therapeutic effects or action targets so as to broad application of these two ginsengs.
Ultrashort pulse laser ablation of dielectrics: Thresholds, mechanisms, role of breakdown
Authors: Mirza Inam , Bulgakova Nadezhda M. , Tomáštík Jan , Michálek Václav , Haderka Ondřej , Fekete Ladislav , Mocek Tomáš
Published: December 2016

In this paper, we establish connections between the thresholds and mechanisms of the damage and white-light generation upon femtosecond laser irradiation of wide-bandgap transparent materials. On the example of Corning Willow glass, evolution of ablation craters, their quality, and white-light emission were studied experimentally for 130-fs, 800-nm laser pulses. The experimental results indicate co-existence of several ablation mechanisms which can be separated in time. Suppression of the phase explosion mechanism of ablation was revealed at the middle of the irradiation spots. At high laser fluences, air ionization was found to strongly influence ablation rate and quality and the main mechanisms of the influence are analysed. To gain insight into the processes triggered by laser radiation in glass, numerical simulations have been performed with accounting for the balance of laser energy absorption and its distribution/redistribution in the sample, including bremsstrahlung emission from excited free-electron plasma. The simulations have shown an insignificant role of avalanche ionization at such short durations of laser pulses while pointing to high average energy of electrons up to several dozens of eV. At multi-pulse ablation regimes, improvement of crater quality was found as compared to single/few pulses.
Strain tunable magnetism in SnX2 (X = S, Se) monolayers by hole doping
Authors: Xiang Hui , Xu Bo , Xia Yidong , Yin Jiang , Liu Zhiguo
Published: December 2016

By first-principles calculations, the magnetism of hole doped tin dichalcogenides SnX2 (X = S, Se) monolayers is systematically studied. It is found that a phase transition from nonmagnetic to ferromagnetic ground state appears once above the critical hole density (~1014 cm−2). The spin magnetic moment can maintain a magnitude of 1.0 μB/hole with excellent stability of ferromagnetic state. Furthermore, we demonstrate that strain is very useful to modulate the DOS near the valence band, resulting in the reduction of the critical hole density to ~1013 cm−2 when the strain reaches 4% (6%) in SnS2 (SnSe2), which can be realized in common field effect transistors. Moreover, the phonon dispersion calculations for the strained SnX2 monolayers indicate that they can keep the dynamical stability under the hole doping. Therefore, the strain tunable magnetic transition in hole doped tin dichalcogenides indicates their potential promising applications in spintronic devices.
Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways
Authors: Park Jeongyeon , Lee Hyunyoung , Lee Hae June , Kim Gyoo Cheon , Kim Do Young , Han Sungbum , Song Kiwon
Published: December 2016

Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.
Deconstructing the Cognitive Estimation Task: A Developmental Examination and Intra-Task Contrast
Authors: Silverman Sarit , Ashkenazi Sarit
Published: December 2016

The cognitive estimation task (CET) requires participants to answer estimation questions that lack definitive answers. Few studies examine CET performance in healthy populations, and even fewer in children. Previous research has not considered differences between categories within the CET. The categories differ in their reliance on units of measurement, which is significant when examining CET performance in children due to educational factors. The goal of the study was to examine CET performance in 10 and 12 year-old children and contrast the CET categories. We found a developmental effect in overall CET performance: children’s performance was more extreme than adults but no differences were found between the groups of children. Examination of the CET categories revealed differential developmental trajectories: the children’s scores were more extreme in weight and time, while comparable to adults in quantity. We conclude that CET questions that require application of units of measurement are more difficult for childern due to higher involvement of executive functions, and children have less experience applying them in daily life. The CET is not a unified construct and has the potential to shed light on how children acquire an understanding of magnitudes and units of measurement.
PGRP negatively regulates NOD-mediated cytokine production in rainbow trout liver cells
Authors: Jang Ju Hye , Kim Hyun , Jang Mi Jung , Cho Ju Hyun
Published: December 2016

Pattern-recognition receptors (PRRs) initiate innate immunity via pathogen recognition. Recent studies suggest that signalling pathways downstream of different PRRs and their crosstalk effectively control immune responses. However, the cross-regulation among PRRs and its effects have yet to be fully described in fish. Here, we examined the crosstalk between OmPGRP-L1, a long form of PGRP in rainbow trout, and other PRRs during pathogenic infections. OmPGRP-L1 expression was increased in RTH-149 cells by iE-DAP and MDP, which are agonists of NOD1 and NOD2, respectively. The silencing of NOD1 and NOD2 specifically inhibited the upregulation of OmPGRP-L1 expression induced by their cognate ligands. Suppression of RIP2 and NF-κB activation prevented the induction of OmPGRP-L1 expression. An in silico analysis and electrophoretic mobility shift assay revealed that the promoter of OmPGRP-L1 has NF-κB binding sites, suggesting that OmPGRP-L1 is produced through the NOD-RIP2-NF-κB signalling pathway. Loss-of-function and gain-of-function experiments indicated that OmPGRP-L1 downregulates the induction of NOD-mediated pro-inflammatory cytokine expression. Mechanistically, secreted OmPGRP-L1 inhibited the activation of the NOD-induced NF-κB pathway via downregulation of TAK1 and IκBα phosphorylation through A20 expression. Our data demonstrate that OmPGRP-L1 and NODs might play interdependent roles in the inflammatory response to bacterial infections in rainbow trout.
Demonstration of Complementary Ternary Graphene Field-Effect Transistors
Authors: Kim Yun Ji , Kim So-Young , Noh Jinwoo , Shim Chang Hoo , Jung Ukjin , Lee Sang Kyung , Chang Kyoung Eun , Cho Chunhum , Lee Byoung Hun
Published: December 2016

Strong demand for power reduction in state-of-the-art semiconductor devices calls for novel devices and architectures. Since ternary logic architecture can perform the same function as binary logic architecture with a much lower device density and higher information density, a switch device suitable for the ternary logic has been pursued for several decades. However, a single device that satisfies all the requirements for ternary logic architecture has not been demonstrated. We demonstrated a ternary graphene field-effect transistor (TGFET), showing three discrete current states in one device. The ternary function was achieved by introducing a metal strip to the middle of graphene channel, which created an N-P-N or P-N-P doping pattern depending on the work function of the metal. In addition, a standard ternary inverter working at room temperature has been achieved by modulating the work function of the metal in a graphene channel. The feasibility of a ternary inverter indicates that a general ternary logic architecture can be realized using complementary TGFETs. This breakthrough will provide a key stepping-stone for an extreme-low-power computing technology.
Statistical Characterization of the Medical Ultrasound Echo Signals
Authors: Cai Runqiu
Published: December 2016

Medical ultrasound echo signals provide the basic information for obtaining the ultrasonic image in medical ultrasound technology. The statistics of the ultrasound echo signals reveals the systematic structure of the medical ultrasonic image via analyzing the corresponding statistical distributions. A novel statistical distribution, the ascending order K distribution, was proposed here to model the medical ultrasound echo signals. The ascending order K distribution was developed in light of the statistical analysis of the sequential waveforms in the echo signals. Comparison with the previous statistical distributions was made to verify the superiority of the ascending order K distribution. Further discussion showed the determination of the statistical principles for the ultrasound signals can enhance our understanding of the statistical principles of the ultrasound imaging, and thus, facilitate the optimization of the ultrasound image and the tissue identification in the ultrasound diagnosis.
Tuning apparent friction coefficient by controlled patterning bulk metallic glasses surfaces
Authors: Li Ning , Xu Erjiang , Liu Ze , Wang Xinyun , Liu Lin
Published: December 2016

Micro-honeycomb structures with various pitches between adjacent cells were hot-embossed on Zr35Ti30Cu8.25Be26.75 bulk metallic glass surface. The effect of pitch geometry on the frictional behavior of metallic glass surface was systematically investigated. The results revealed that all textured metallic glass surfaces show a reduction in friction coefficient compared to smooth surface. More intriguingly, the friction coefficient first decreased and then increased gradually with increasing pitches. Such unique behavior can be understood fundamentally from the perspective of competing effects between contact area and local stress level with increasing pitches. This finding not only enhance the in-depth understanding of the mechanism of the significant role of surface topography on the frictional behavior of metallic glass surface, but also opens a new route towards other functional applications for bulk metallic glasses.
Synthesis of single-crystal low-loss LiB3O5 nanowire and its optical properties
Authors: Qu Guang-Yuan , Yang Cheng-Wei , Cui Guo-xin , Zhang Xue-Jin , Lu Yan-Qing
Published: December 2016

Optical-quality single-crystal LiB3O5 (LBO) nanowires are synthesized for the first time using a sol–gel method. The LBO nanowires possess diameters ranging from 200 to 800 nm and lengths of up to 200 μm, and exhibit excellent uniformity, smooth surfaces, and good mechanical properties. A typical propagating loss of 0.038 dB/μm at 532 nm is obtained for a 620 nm-diameter nanowire. This is a decrease of one order of magnitude compared with that of a β-BaB2O4 (BBO) nanowire with similar diameter, which makes the LBO nanowire a promising candidate to construct miniaturized nonlinear photonic devices.
Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa
Authors: Loo Shining , Kam Antony , Xiao Tianshu , Nguyen Giang K. T. , Liu Chuan Fa , Tam James P.
Published: December 2016

Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor against porcine pancreatic elastase was first identified from the squash family in 1989. Here, we report the identification and characterization of a knottin-type human neutrophil elastase inhibitor from Hibiscus sabdariffa of the Malvaceae family. Combining proteomic and transcriptomic methods, we identified a panel of novel cysteine-rich peptides, roseltides (rT1-rT8), which range from 27 to 39 residues with six conserved cysteine residues. The 27-residue roseltide rT1 contains a cysteine spacing and amino acid sequence that is different from the squash knottin-type elastase inhibitor. NMR analysis demonstrated that roseltide rT1 adopts a cystine-knot fold. Transcriptome analyses suggested that roseltides are bioprocessed by asparagine endopeptidases from a three-domain precursor. The cystine-knot structure of roseltide rT1 confers its high resistance against degradation by endopeptidases, 0.2 N HCl, and human serum. Roseltide rT1 was shown to inhibit human neutrophil elastase using enzymatic and pull-down assays. Additionally, roseltide rT1 ameliorates neutrophil elastase-stimulated cAMP accumulation in vitro. Taken together, our findings demonstrate that roseltide rT1 is a novel knottin-type neutrophil elastase inhibitor with therapeutic potential for neutrophil elastase associated diseases.