bluuebunny/crossref_metadata_2025 huggingface dataset:
Phylogeography of Paramuricea: The Role of Depth and Water Mass in the Evolution and Distribution of Deep-Sea Corals
Authors: Quattrini Andrea M. , Herrera Santiago , Adams J. Mike , Grinyó Jordi , Allcock A. Louise , Shuler Andrew , Wirshing Herman H. , Cordes Erik E. , McFadden Catherine S.
Published: April 2022

The processes that control diversification and speciation in deep-sea species are poorly known. Here, we analyzed data produced by Restriction-Site Associated DNA Sequencing (RAD-Seq) of octocorals in the genus Paramuricea to elucidate diversification patterns and examine the role of environmental gradients in their evolution. The genus Paramuricea evolved around 8 MYA, with a high probability of a broad ancestral depth range from mesophotic depths to the deep sea. At around 1-2 MYA, the genus diversified across the continental slope of the deep North Atlantic, supporting the depth-differentiation hypothesis, with no invasions back into shallower depths (< 200 m). Diversification in the deep sea generally occurred from shallower, warmer waters to deeper, colder depths of the lower continental slope. We also found that the vertical structure of water masses was influential in shaping phylogeographic patterns across the North Atlantic Ocean, with clades found in either upper/intermediate or intermediate/deep water masses. Our data suggest that species diverged first because of environmental conditions, including depth, temperature, and/or water mass, and then diversified into different geographical regions multiple times. Our results highlight the role of the environment in driving the evolution and distribution of Paramuricea throughout the deep sea. Furthermore, our study supports prior work showing the utility of genomic approaches over the conventionally-used DNA barcodes in octocoral species delimitation.
Fire Behaviour of Treated Insulation Fibreboards and Predictions of its Future Development Based on Natural Aging Simulation
Authors: Osvaldová Linda Makovická , Mitrenga Patrik , Jancík Juraj , Titko Michal , Efhamisisi Davood , Košútová Katarína
Published: April 2022

Wood-based composite materials represent a major fire safety risk. The application of water-based fire retardants (FR) can improve their fire performance. However, it is important to investigate the performance and stability of this protective system over time, as the ageing of materials and their exposure to climatic conditions can change their properties. The aim of this research work was to evaluate the effect of long-term aging on the combustibility of low-density insulation fibreboards (IFs) treated with a water-soluble flame retardant (FR) based on inorganic salts. Both natural and accelerated aging simulations were conducted. The accelerated aging simulation was performed using climate chambers. The FR efficiency was evaluated by the mass loss rate of the tested specimen. As expected, the FR formulation used significantly enhanced the fire properties of the boards, reaching maximum efficiency 6 months after its application onto the fibreboards. Markedly, after 4 years the efficiency of the investigated FR additive was considerably decreased with weight loss values comparable with the values of the untreated samples. Markedly, besides the apparent protection of wood fibers, the retardant did not prevent the gradual increase in mass loss rate of samples, it just slowed down the process. The main result of the study is to determine the effectiveness of the investigated FR on IFs during their natural ageing. The development of accurate datasets on the thermal behaviour of IFs is critical for their proper utilisation in building and construction applications.
Cluster Differences in Antibiotic Resistance, Biofilm Formation, Mobility, and Virulence of Clinical Enterobacter cloacae Complex
Authors: Liu Shixing , Chen Liqiong , Wang Lingbo , Zhou Beibei , Ye Dandan , Zheng Xiangkuo , Lin Yishuai , Zeng Weiliang , Zhou Tieli , Ye Jianzhong
Published: April 2022

Due to the lack of research on the characteristics of different clusters of Enterobacter cloacae complex (ECC), this study aimed to characterize and explore the differences among species of the ECC. An analysis based on hsp60 showed that Enterobacter hormaechei was predominant in ECC. Interestingly, the antibiotic resistance rates of clusters were different, among which E. hormaechei subsp. steigerwaltii (cluster VIII) and Enterobacter cloacae IX (cluster IX) possessed high resistant rates to ciprofloxacin and levofloxacin, but cluster II (Enterobacter kobei) had low resistant rates. Cluster II exhibited a strong biofilm formation ability. Different motility and protease production ability were shown for distinct clusters. A PCR analysis showed that clusters I, III, VI, VIII, and IX carried more virulence genes, while cluster II had fewer. Clusters I, VIII, and IX with high pathogenicity were evaluated using the Galleria mellonella infection model. Thus, the characteristics of resistance, biofilm-forming ability, mobility, and virulence differed among the clusters. The strains were divided into 12 subgroups based on hsp60. The main clusters of ECC clinical strains were I, II, III, VI, VIII, and IX, among which IX, VIII, and I were predominant with high resistance and pathogenicity, and cluster II (E. kobei) was a special taxon with a strong biofilm formation ability under nutrient deficiency, but was associated with low resistance, virulence, and pathogenicity. Hence, clinical classification methods to identify ECC subgroups are an urgent requirement to guide the treatment of clinical infections.
Planktonic Aggregates as Hotspots for Heterotrophic Diazotrophy: The Plot Thickens
Authors: Riemann Lasse , Rahav Eyal , Passow Uta , Grossart Hans-Peter , de Beer Dirk , Klawonn Isabell , Eichner Meri , Benavides Mar , Bar-Zeev Edo
Published: April 2022

Biological dinitrogen (N2) fixation is performed solely by specialized bacteria and archaea termed diazotrophs, introducing new reactive nitrogen into aquatic environments. Conventionally, phototrophic cyanobacteria are considered the major diazotrophs in aquatic environments. However, accumulating evidence indicates that diverse non-cyanobacterial diazotrophs (NCDs) inhabit a wide range of aquatic ecosystems, including temperate and polar latitudes, coastal environments and the deep ocean. NCDs are thus suspected to impact global nitrogen cycling decisively, yet their ecological and quantitative importance remain unknown. Here we review recent molecular and biogeochemical evidence demonstrating that pelagic NCDs inhabit and thrive especially on aggregates in diverse aquatic ecosystems. Aggregates are characterized by reduced-oxygen microzones, high C:N ratio (above Redfield) and high availability of labile carbon as compared to the ambient water. We argue that planktonic aggregates are important loci for energetically-expensive N2 fixation by NCDs and propose a conceptual framework for aggregate-associated N2 fixation. Future studies on aggregate-associated diazotrophy, using novel methodological approaches, are encouraged to address the ecological relevance of NCDs for nitrogen cycling in aquatic environments.
Perspectives on Development of Measures to Estimate Career Blast Exposure History in Service Members and Veterans
Authors: Turner Stephanie M. , Sloley Stephanie S. , Bailie Jason M. , Babakhanyan Ida , Gregory Emma
Published: April 2022

The Department of Defense (DOD) has recently prioritized the investigation of the acute and chronic adverse brain health and performance effects of low-level blast (LLB) generated by the use of weapons systems. While acute exposure can be quantified by sensor technology, career exposure has no widely accepted and validated measure for characterization. Currently, distinct research groups are developing and validating four promising measures to estimate career blast exposure history: the Salisbury Blast Interview, Blast Exposure Threshold Survey, Blast Ordnance and Occupational Exposure Measure, and the Blast Frequency and Symptom Severity. Each measure offers an assessment of blast history that is uniquely beneficial to addressing specific research questions. However, use of divergent strategies is not efficient to accelerate the field's understanding of the impact of career exposure and Service-connected health outcomes. As a DOD-wide solution, collaboration across these groups is required to develop a tool(s) that can be standardized across research studies and, ultimately, pared down to be implemented in clinical settings. Here, we overview the current four measures and provide a perspective on the way forward for optimization and/or combination in support of this solution.
Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study
Authors: Belltall Amparo , Mazzinari Guido , Garrido-Cano Iris , Giner Francisco , Marí Anabel Marqués , Eroles Pilar , Argente-Navarro María Pilar , Cata Juan Pablo , Diaz-Cambronero Oscar
Published: April 2022

Background

There is growing interest in the possible effect of perioperative anesthetic management on the growth and spread of cancer. The impact of perioperative use of opioids on cancer recurrence remains controversial and an assessment cannot yet be established based on current publications. This study aimed to assess the differential expression of opioid receptors between healthy and tumor tissues in patients with stage II and III colorectal cancer undergoing elective surgery by immunohistochemistry (IHC).

Methods

Propensity–score matched case–control study nested in a retrospective cohort of patients with stage II or III colorectal. The primary endpoint was the difference in µ–opioid receptor (MOR) expression measured by IHC between tumor and healthy tissue in subject with or without recurrence. Secondary endpoints were to evaluate the differences in Opioid Growth Factor Receptor (OGFR), cyclic adenosine monophosphate (cAMP) production and protein kinase A (PKA) in the matched sample and from a from samples of colorectal cancer stored in the Cancer Genome Atlas (TCGA) and Genotype Tissue Expression Project (GTEx).

Results

There was a significant difference in MOR receptor (median 3 [intequartile range IQR: 1–3] and 0 [IQR: 0–2], P<0.001) and OGFR receptor (median 6 [IQR: 5–6] and 2 [IQR: 1–2], P<0.001) in tumor and control tissue respectively. However, there were no significant differences in cAMP nor PKA expression between both types of tissues and in expression in any of the analyzed variables by recurrence status. The MOR and OGFR expression data from TCGA database were similar to our sample size data with lower expression of MOR and higher expression of OGFR in tumoural samples with a skewed distribution for MOR expression in tumor tissue both in patients with and without recurrence.

Conclusion

In patients with stage II and III colorectal cancer, overall expression of MOR and OGFR was significantly increased but was not different between previously matched patients with or without recurrence. No differences were found in the analyzed metabolic pathway of cAMP–PKA: These results were confirmed by an in silico analysis of samples from the TCGA–GTEx database.
Pharmacological Properties of Ginsenoside Re
Authors: Gao Xiao-Yan , Liu Guan-Cheng , Zhang Jian-Xiu , Wang Ling-He , Xu Chang , Yan Zi-An , Wang Ao , Su Yi-Fei , Lee Jung-Joon , Piao Guang-Chun , Yuan Hai-Dan
Published: April 2022

Ginsenoside Re is a protopanaxatriol-type saponin extracted from the berry, leaf, stem, flower bud, and root of Panax ginseng. In recent years, ginsenoside Re (Re) has been attracting attention as a dietary phytochemical. In this review, studies on Re were compiled by searching a combination of keywords, namely “pharmacology,” “pharmacokinetics,” and “toxicology,” in the Google Scholar, NCBI, PubMed, and Web of Science databases. The aim of this review was to provide an exhaustive overview of the pharmacological activities, pharmacokinetics, and toxicity of Re, focusing on clinical evidence that has shown effectiveness in specific diseases, such as diabetes mellitus, nervous system diseases, inflammation, cardiovascular disease, and cancer. Re is also known to eliminate virus, enhance the immune response, improve osteoporosis, improve skin barrier function, enhance intracellular anti-oxidant actions, regulate cholesterol metabolism, alleviate allergic responses, increase sperm motility, reduce erectile dysfunction, promote cyclic growth of hair follicles, and reduce gastrointestinal motility dysfunction. Furthermore, this review provides data on pharmacokinetic parameters and toxicological factors to examine the safety profile of Re. Such data will provide a theoretical basis and reference for Re-related studies and future applications.
Target-Based Virtual Screening of Natural Compounds Identifies a Potent Antimalarial With Selective Falcipain-2 Inhibitory Activity
Authors: Uddin Amad , Gupta Sonal , Mohammad Taj , Shahi Diksha , Hussain Afzal , Alajmi Mohamed F. , El-Seedi Hesham R. , Hassan Imtaiyaz , Singh Shailja , Abid Mohammad
Published: April 2022

We employed a comprehensive approach of target-based virtual high-throughput screening to find potential hits from the ZINC database of natural compounds against cysteine proteases falcipain-2 and falcipain-3 (FP2 and FP3). Molecular docking studies showed the initial hits showing high binding affinity and specificity toward FP2 were selected. Furthermore, the enzyme inhibition and surface plasmon resonance assays were performed which resulted in a compound ZINC12900664 (ST72) with potent inhibitory effects on purified FP2. ST72 exhibited strong growth inhibition of chloroquine-sensitive (3D7; EC50 = 2.8 µM) and chloroquine-resistant (RKL-9; EC50 = 6.7 µM) strains of Plasmodium falciparum. Stage-specific inhibition assays revealed a delayed and growth defect during parasite growth and development in parasites treated with ST72. Furthermore, ST72 significantly reduced parasite load and increased host survival in a murine model infected with Plasmodium berghei ANKA. No Evans blue staining in ST72 treatment indicated that ST72 mediated protection of blood–brain barrier integrity in mice infected with P. berghei. ST72 did not show any significant hemolysis or cytotoxicity against human HepG2 cells suggesting a good safety profile. Importantly, ST72 with CQ resulted in improved growth inhibitory activity than individual drugs in both in vitro and in vivo studies.
Coumarin-Mediated Growth Regulations, Antioxidant Enzyme Activities, and Photosynthetic Efficiency of Sorghum bicolor Under Saline Conditions
Authors: Sultana Robina , Wang Xiukang , Azeem Muhammad , Hussain Tabassum , Mahmood Athar , Fiaz Sajid , Qasim Muhammad
Published: April 2022

Secondary metabolites, such as phenolic compounds, play an important role in alleviating salinity-induced negative effects in plants. The present study focused on seed priming and foliar application of a potent phenolic compound, coumarin, to induce salinity tolerance inSorghum bicolorvar. SS-77. Based on pilot experiment, 100 mg L−1concentration of coumarin was applied to mitigate the negative effects of salinity on Sorghum, grown at 0, 100, and 200 mM NaCl under netted greenhouse conditions. Coumarin was applied to each salinity treatment in four different ways (i) non-primed control (NP), (ii) seed priming (COP), (iii) foliar application (COF), and (iv) a combination of seed priming and foliar application (COPF). Salinity stress significantly reduced the plant growth, biochemical attributes, and photosynthetic efficiency of Sorghum, whereas coumarin treatments (COP, COF, and COPF) showed a significant increase (P< 0.01) in above-mentioned parameters at all salinities. Among all, the combined treatment (COPF) showed maximum increase in growth, biochemicals, photosynthetic pigments, antioxidant enzymes, and photosynthetic efficiency parameters. Therefore, it is suggested that a combination of seed priming and foliar spray of 10 mg L−1coumarin is more suitable than their individual applications. It is an environment friendly and economically feasible approach that will be used to improve salinity tolerance of Sorghum and helpful to get considerable biomass from saline degraded lands to fulfill food, fodder, and energy demands of the ever-growing population.
Role of Circadian Rhythms in Major Plant Metabolic and Signaling Pathways
Authors: Venkat Ajila , Muneer Sowbiya
Published: April 2022

Plants require an endogenous regulatory network and mechanism to cope with diurnal environmental changes and compensate for their sessile nature. Plants use the circadian clock to anticipate diurnal changes. Circadian rhythm predicts a 24-h cycle with 16 h of light and 8 h of darkness in response to abiotic and biotic factors as well as the appropriate temperature. For a plant’s fitness, proper growth, and development, these rhythms synchronize the diurnal photoperiodic changes. Input pathway, central oscillator, and output pathway are the three components that make up the endogenous clock. There are also transcriptional and translational feedback loops (TTFLs) in the clock, which are dependent on the results of gene expression. Several physiological processes, such as stress acclimatization, hormone signaling, morphogenesis, carbon metabolism, and defense response, are currently being investigated for their interactions with the circadian clock using phenotypic, genomic, and metabolic studies. This review examines the role of circadian rhythms in the regulation of plant metabolic pathways, such as photosynthesis and carbon metabolism, as well as developmental and degenerative processes, such as flowering and senescence. Furthermore, we summarized signaling pathways related to circadian rhythms, such as defense response and gene regulatory pathways.
Reliving the Old Dream: Rural Tourism Autobiographical Memory on Behavioral Intention
Authors: Zhao Zhifeng , Li Zhiwei , Chen Cai
Published: April 2022

This paper evaluates a theoretical model based on hypothesized relationships among four constructs, namely, autobiographical memory (memory rehearsal and memory impact), and place attachment as antecedents of revisit intention and recommendation intention in the context of rural tourism in China. The results of 301 Chinese tourists show that the two dimensions of tourists’ autobiographical memory (memory rehearsal and memory impact) affect the tourists’ intention to revisit and recommend. Place attachment plays an intermediary role among tourists’ autobiographical memory, revisit intention, and recommendation intention. This study is the first to apply the structural dimension of autobiographical memory has been applied to rural tourism in China. Theoretical and managerial implications are discussed based on the study results.
The Path of College Students’ Entrepreneurship Education Under Causal Attribution Theory From the Perspective of Entrepreneurial Psychology
Authors: Wang Changlin , Liu Qingquan , Li Hongming , Liu Yuanbing
Published: April 2022

The purpose of the study is to promote college students to actively respond to the national “Public Entrepreneurship and Mass Innovation” policies and calls, improve college students’ entrepreneurial enthusiasm and their entrepreneurial ability, and cultivate their good entrepreneurial psychological states. First, the relevant content of entrepreneurship psychology and causal attribution theory is displayed. Second, the questionnaire of college students’ entrepreneurship education is formulated and a questionnaire survey is conducted on University N based on the relevant content of entrepreneurship psychology. Subsequently, the management system of new venture A is taken as the research object to construct the management strategy of new ventures and simulate the implementation process. Finally, the questionnaire survey results of college students’ entrepreneurship education are analyzed and the corresponding entrepreneurship education path is formulated. Meanwhile, the implementation effect of the management strategy of new ventures is evaluated. After the questionnaire is sorted out, it is found that there are some problems in college students’ entrepreneurship education, such as weak awareness of entrepreneurship, insufficient publicity, outdated curriculum, and unqualified teachers. The reasons for these problems are the constraints of traditional concepts, insufficient attention, and incomplete system construction. Therefore, a plan is made for overall entrepreneurship education, the publicity of the concept of entrepreneurship education is strengthened, and the setting of entrepreneurship education curriculum and the ability of the teachers for entrepreneurship education are improved. Through the evaluation of the simulation implementation of a new enterprise management strategy, it is found that the new management strategy can achieve the expected effect. Therefore, this study provides some references for the development of college students’ entrepreneurship education and the management strategy of new ventures.
Data Visualization and Analysis in Second Language Research
Authors: Sun Yiou , Wang Ping
Published: April 2022

I am working as both a TEFL teacher and an SLA researcher in China, doing SLA research. Recently, I have been working on new approaches to data analysis and I’ve found that a book titled “Data Visualization and Analysis in Second Language Research” by Dr. Guilherme D. Garcia is of great significance in empirical research in the field of SLA. This book serves only as a practical and user-friendly guide to beginners involved in SLA research, but also navigation to veteran SLA researchers devoted to new perspectives in data analysis. So far, the author has made his first attempt to connect data visualization under R to SLA research. The author reviews the previous research results and suggests some modifications through running R. From my perspective, it fills a gap in the SLA research practice—to lead readers into a new track of visualizing their reported results. Overall, to make an increasingly higher demand for SLA research, this book is particularly designated for quantitative data analysis in SLA. It is strongly recommended to write a book review to introduce readers to the realm of data visualization and help most SLA researchers better understand the potential value of R and visualization in SLA research.
Pump It Up!—Strong Muscles Can Make You Healthier
Authors: Peña Javier , Señé-Mir Anna M. , Chulvi-Medrano Iván , Rial Tamara , Faigenbaum Avery D.
Published: April 2022

We imagine that you want to be healthy; nobody likes to be ill because that often prevents us from doing the things we like to do. However, we often do not do everything in our power to keep ourselves healthy because modern life is designed to be comfortable. Consequently, we are moving and exercising less than ever, and even young people are suffering the effects of physical inactivity. In this article, we will explain why it is essential for you to move your body, to do so correctly, and to be physically active throughout your life. We will also dispel some myths about children and adolescents lifting weights. There are many things you can do in your daily life to be more physically active!
Biohardening of Banana cv. Karpooravalli (ABB; Pisang Awak) With Bacillus velezensis YEBBR6 Promotes Plant Growth and Reprograms the Innate Immune Response Against Fusarium oxysporum f.sp. cubense
Authors: Saravanan R. , Nakkeeran S. , Saranya N. , Kavino M. , Ragapriya V. , Varanavasiappan S. , Raveendran M. , Krishnamoorthy A. S. , Malathy V. G. , Haripriya S.
Published: April 2022

Graphical Abstract

Induction of innate immune response and growth promotion in banana by B. velezensis against Foc.
Parecoxib Reduced Postsurgical Pain and Facilitated Movement More Than Patient Controlled Analgesia
Authors: Chiu Szu-Ching , Livneh Hanoch , Chen Jin-Cheng , Chang Chia-Ming , Hsu Honda , Chiang Tsay-I , Tsai Tzung-Yi
Published: April 2022

Background

Postoperative pain management is an imperative issue for patients undergoing lumbar spinal fusion surgery. Delayed pain relief is associated with poor clinical outcomes. This study compared the effects of intravenously administered patient-controlled analgesia (PCA) with intravenous parecoxib, both commonly used methods for analgesic pain control after surgery.

Methods

A non-randomized study was used to recruit 68 patients who were scheduled to receive lumbar spinal fusion surgery at a hospital in Taiwan from April through December of 2020. The group treated with parecoxib received an initial perioperative dose of parecoxib 40 mg during a 30-min period and then postoperative intravenous parecoxib at 40 mg per 12-h period, for 72 h. Those with PCA received morphine (0.4 mg/ml), droperidol (0.02 mg/ml), diphenhydramine (0.48 mg/ml), midazolam (0.02 mg/ml) and saline solution during the 3-day study course. Major outcomes, including visual scale pain score and Barthel index of activities of daily living, were collected via review of medical records at 4 times: 12, 24, 48 and 72 h after surgery. Comparative effects between two groups were assessed by the generalized estimating equations.

Results

After adjusting for potential confounders, the administration of parecoxib was associated with a significant decrease in pain scores and an increase in the Barthel Index, when compared with the PCA group (all p < 0.05). Notably, both effects would maintain for 72 h after surgery.

Discussion

This is the first trial of which the authors are aware, that supports intravenous parecoxib as significantly enhancing patient mobility, in addition to having pain control efficacy, when compared with PCA. This study could be used as a reference when instituting interventions to improve the adaptation process and clinical prognoses after lumbar spinal fusion surgery.
How to Identify the Indications for Early Intervention in Acute Necrotizing Pancreatitis Patients: A Long-Term Follow-Up Study
Authors: Lu Jiongdi , Cao Feng , Zheng Zhi , Ding Yixuan , Qu Yuanxu , Mei Wentong , Guo Yulin , Feng Yu-Lu , Li Fei
Published: April 2022

Aim

To explore the indications for early intervention in patients with acute necrotizing pancreatitis (ANP) and evaluate the effect of early intervention on the prognosis of ANP patients.

Methods

The clinical data of patients with ANP who underwent general surgery at Xuanwu Hospital of Capital Medical University from January 1, 2014, to December 31, 2020, were collected retrospectively. The patients were followed-up every 6 months after discharge, and the last follow-up date was June 30, 2021.

Results

A total of 98 patients with ANP were included in the study. They were divided into an early group (n= 43) and a delayed group (n = 55) according to the first percutaneous drainage (PCD) intervention time (≤ 4 weeks or > 4 weeks). Body temperature, inflammatory factor levels, and the number of patients with persistent organ failure (POF) were higher in the early group than in the delayed group. After the minimally invasive intervention, the body temperature and inflammatory factors of the two groups decreased significantly, most patients with POF improved, and the number of patients with reversal of POF in the early group was higher than that in the delayed group. Although the patients in the early group required more surgical intervention than those in the delayed group, there was no significant difference in mortality, incidence of postoperative complications, total length of hospital stay, or operation cost between the two groups. During long-term follow-up, there was no significant difference in the incidence of short-term and long-term complications and overall survival between the two groups.

Conclusions

Compared to patients in the delayed group, early intervention did not affect the prognosis of patients with ANP. It may be more suitable for patients with ANP with deterioration [such as POF or infected pancreatic necrosis (IPN)].
Colocolic Intussusception in Children: A Case Series and Review of the Literature
Authors: Yan Jiayu , Shen Qiulong , Peng Chunhui , Pang Wenbo , Chen Yajun
Published: April 2022

Background

Colocolic intussusception is a rare subtype of intussusception mostly caused by juvenile polyps. The treatment of colocolic intussusception caused by other pathologic lead points in children remains poorly understood.

Method

A systematic literature review was performed between January 2000 and June 2021 to characterize the comprehensive treatment of colocolic intussusception in children. This report also included patients admitted to our center between January 2010 and June 2021 who were not previously reported in the literature.

Results

We identified 27 patients in 20 studies in addition to 17 patients from our center for a total of 44 patients (median age, 4.4 years; 52.3% male). The lead point was identified in 40 patients (40/44, 90.9%). The most common lead point was juvenile polyps (19/44, 43.2%). A therapeutic enema was performed in 15 patients with colocolic intussusception caused by juvenile polyps and was successful in 9 patients (9/15, 60.0%). Colonoscopic polypectomy was subsequently performed in 8 patients and was successful in 7 patients (7/8, 87.5%). The other patient had undergone laparoscopic exploration, and no abnormality was found. Subsequently, the patient underwent open surgery. The patients with colocolic intussusception caused by other pathologic lead points almost underwent surgical treatment (15/21, 71.4%), including 13 open surgeries and 2 laparoscopic surgeries.

Conclusion

A therapeutic enema followed by colonoscopic polypectomy is feasible to treat colocolic intussusception caused by juvenile polyps unless the patient has bowel perforation; however, surgery is sometimes needed. For patients with colocolic intussusception caused by other pathologic lead points, open surgery remains the primary treatment.
Comparison of Five Protocols of Estrous Synchronization on Reproductive Performance of Hu Sheep
Authors: Yu Xiaojie , Bai Yuanyuan , Yang Jiangfeng , Zhao Xiaokun , Zhang Lei , Wang Jing
Published: April 2022

The purpose of this study is to compare five protocols of estrous synchronization for Hu ewes to obtain the most effective and economical protocol, to apply the advantageous scheme in large-scale sheep farming. Healthy multiparous Hu ewes (n = 150) were randomly divided into five groups, and all ewes were administered fluorogestone acetate (FGA, 45 mg) vaginal sponge. The sponges of the first three groups (Groups I, II, and III) were removed on the 11th day, and 0.1 mg of PGF2α was injected intramuscularly on the ninth day. Group I received 6 μg of gonadotropin-releasing hormone (GnRH) by intramuscular injection at 36th h after withdrawal of the sponge. Group II was injected 330 IU of pregnant mare serum gonadotropin (PMSG) on the ninth day. The combination of 6 μg of GnRH and 330 IU of PMSG was treated in Group III at the same time as Group I and Group II. The sponges of the latter two groups (Groups IV and V) were removed on the 13th day, and 330 IU of PMSG was injected intramuscularly simultaneously. PGF2α (0.1 mg) was administered on the 12th day in Group IV. All ewes were detected for estrus at 24, 36, 48, 60, and 72 h after the sponge removal. The loss of sponge and vaginitis was recorded when the sponge was withdrawn. Cervical artificial insemination (AI) was performed with fresh semen of Dorper rams diluted with skimmed milk. After 30 days of insemination, the conception was detected with a veterinary B-ultrasound scanner. The lambing status of all ewes and the cost of drugs for estrous synchronization in each group were recorded. The results showed the following: (1) on the whole, the average percentage of estrous ewes in the period of 24–36 h and 36–48 h after removal was significantly higher than other three periods and that of the period of 60–72 h was significantly lower than the first three periods after removal; (2) there was no significant difference in percentages of estrous ewes in any of the five time periods, sponge loss rate, vaginitis rate, total percentage of estrous ewes, conception rate, single lambing rate, twinning rate, and multiple lambing rate of ewes among five protocols; (3) total percentage of estrous ewes and conception rate were more than or equal to 80% in the Groups II and III, and the twinning lamb rate of the Group II protocol was 70%; (4) there was no difference in lambing rate of ewes among Groups II, III, IV, and V; (5) the Group III had the highest drug cost of 22.5 CNY. In conclusion, considering the lambing rate, twinning lamb rate, and drug cost for estrous synchronization, Group II was the most advisable for application and promotion in large-scale sheep farms among these five protocols of estrus synchronization.
Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia
Authors: Zhong Huan , Guo Zhongbao , Xiao Jun , Zhang Hong , Luo Yongju , Liang Junneng
Published: April 2022

Circular RNA (circRNA) is an endogenous biomolecule in eukaryotes. It has tissue- and cell-specific expression patterns and can act as a microRNA sponge or competitive endogenous RNA. Although circRNA has been found in several species in recent years, the expression profiles in fish gonad are still not fully understood. We detected the expression of circRNA in the ovary, testis, and sex-changed gonad of tilapia by high-throughput deep sequencing, and circRNA-specific computing tools. A total of 20,607 circRNAs were obtained, of which 141 were differentially expressed in the testis and ovary. Among these circRNAs, 135 circRNAs were upregulated and 6 circRNAs were downregulated in female fish. In addition, GO annotation and KEGG pathway analysis of the host genes of circRNAs indicated that these host genes were mainly involved in adherens junction, androgen production, and reproductive development, such as ZP3, PLC, delta 4a, ARHGEF10, and HSD17b3. It is worth noting that we found that circRNAs in tilapia gonads have abundant miRNA-binding sites. Among them, 935 circRNAs have a regulatory effect on miR-212, 856 circRNAs have a regulatory effect on miR-200b-3p, and 529 circRNAs have a regulatory effect on miR-200b-5p. Thus, our findings provide a new evidence for circRNA–miRNA networks in the gonads in tilapia.
Association Between Cardiovascular Disease Risk Factors and Mortality in Adults With Diabetes: A Stratified Analysis by Sex, Race, and Ethnicity
Authors: Savage Kristina , Williams Joni S. , Garacci Emma , Egede Leonard E.
Published: April 2022

Objectives: To assess sex and racial/ethnic differences in the relationship between multiple cardiovascular disease (CVD) risk factors and mortality among a nationally representative sample of adults with diabetes.

Methods: Data were analyzed from 3,503 adults with diabetes from the National Health and Nutrition Examination Survey 2001–2010 and its linked mortality data through 31 December 2011. The outcome was mortality; the independent variables were sex and race/ethnicity. Covariates included demographics, comorbidity, and lifestyle variables. Cox proportional hazards regression was used to test associations between mortality and CVD risk factors.

Results: In adjusted analyses, the association between diastolic blood pressure and mortality was significantly different by sex and race/ethnicity (unadjusted p = 0.009; adjusted p = 0.042). Kaplan-Meier survival curves showed Hispanic women had the highest survival compared to Hispanic men and Non-Hispanic Black (NHB) and Non-Hispanic White (NHW) men and women; NHW men had the lowest survival probability.

Conclusion: In this nationally representative sample, stratified analyses showed women had higher survival rates compared to men within each race/ethnicity group, and Hispanic women had the highest survival compared to all other groups.
Evaluation of Trichogramma cacaeciae (Hymenoptera: Trichogrammatidae) and Blattisocius mali (Mesostigmata: Blattisociidae) in the Post-Harvest Biological Control of the Potato Tuber Moth (Lepidoptera: Gelechiidae): Use of Sigmoid Functions
Authors: Solano-Rojas Yohan , Gallego Juan R. , Gamez Manuel , Garay Jozsef , Hernandez Joaquin , Cabello Tomas
Published: April 2022

The potato tuber moth (PTM), Phthorimaea operculella, is the main potato pest in the world, having managed to adapt to a wide range of climatic conditions, and causing damage to stored tubers. The effectiveness of a biological control using the parasitoid Trichogramma cacaeciae and the predatory mite Blattisocius mali was evaluated in PTM under conditions that simulated those of tubers in storage. The number of adults found at the end of the trial was significantly higher in the control (181.75 ± 11.00 adults/container) than in treatments where B. mali (24.50 ± 7.22) or T. cacaeciae (102.00 ± 14.61) were released. The biological control’s effectiveness using the Abbot formula was 86.52% for the predator and 43.88% for the parasitoid. In addition, the PTM population data was fitted by logistic models, and the best fit was found for the simple logistic function. Using the data generated from these functions, the control efficiencies for the mite and the parasitoid were 94.85% and 73.77%, respectively. B. mali showed greater potential for being incorporated into integrated PTM management programs than T. cacaeciae. Based on logistic functions, a novel way of estimating the effectiveness of predators and parasitoids is also presented and discussed.
Stubble Quality of Wheat Grown under No-Tillage and Conventional Tillage Systems, and Effects of Stubble on the Fermentation Profile of Grazing Ewes’ Ruminal Fluid
Authors: Guesmi Hajer , Darej Cyrine , Goufo Piebiep , Ben Youssef Salah , Chakroun Mohamed , Ben Salem Hichem , Trindade Henrique , Moujahed Nizar
Published: April 2022

Conservation practices that involve leaving mulch on the soil are known to increase soil fertility. However, mulch is an important source of feed for ruminant livestock. Accordingly, the trade-offs between mulching and stubble uptake by livestock are currently hot topics in the research field. In this study, a comparison between the quality of stubbles of wheat grown in no-tillage and conventional tillage fields and the rumen fermentation characteristics of Barbarine ewes grazing in the two fields was carried out. Samples were collected four times after wheat grain harvesting. Immediately after harvest, stubble biomass was slightly higher under no-tillage than conventional tillage. The ewes displayed different grazing behavior, with feeding at a higher rate on the conservative stubble than the conventional stubble. This feeding behavior seemed to be an adaptive strategy to offset the decline in the nutritional quality of stubbles from no-tillage-grown wheat. Indeed, dry matter, protein, fiber, and ash contents were lower in stubbles from no-tillage than conventional tillage at the second sampling time. Consequently, the concentration of the volatile fatty acid, propionic acid, was lower in ewes grazing in the conservative plots. However, this feeding behavior did not cause any body weight impairment during the two-month experimental period. At the end of grazing, the no-tillage practice was found to be comparable to the conventional tillage system according to the amount of residue remaining on the soil surface. Therefore, no-tillage could not be used to solve the competition between crops and livestock for residues.
Phytic Acid Content of Faba Beans (Vicia faba)—Annual and Varietal Effects, and Influence of Organic Cultivation Practices
Authors: Zehring Jenny , Walter Sinja , Quendt Ulrich , Zocher Kathleen , Rohn Sascha
Published: April 2022

Legumes such as faba beans (Vicia faba) are once again gaining popularity, especially in Europe. This is due to the fact that they are an important source of plant-based proteins for human as well as animal nutrition. In addition to a high protein content, faba beans have a wide range of secondary plant metabolites (SPMs). Some of them, such as phytic acid (PA, inositol hexakisphosphate), are discussed controversially with regard to their role as dietary compounds. As ecophysiological conditions and agronomical practices are well known to alter SPMs in (food) plants, it is hypothesized that the farming system has an impact on the overall SPMs content in plants and there might be a correlation between organically grown bean samples and PA content. Consequently, this study aimed at characterizing the German-wide variation in the PA content of faba beans produced under real cultivation conditions. Influencing factors such as cultivar and use of organic or conventional cultivation have been evaluated in order to reveal dependencies of PA in legumes. All bean samples were obtained from different conventional and organic farms from eleven German federal states over three consecutive cultivation years (2016–2018). However, beans did not show annual effects in PA content. As expected, there were dependencies related to the cultivar. Furthermore, significant differences between conventionally and organically grown beans were found, independent of fungicide or insecticide use.
Weed Composition in Hungarian Phacelia (Phacelia tanacetifolia Benth.) Seed Production: Could Tine Harrow Take over Chemical Management?
Authors: Pinke Gyula , Giczi Zsolt , Vona Viktória , Dunai Éva , Vámos Ottilia , Kulmány István , Koltai Gábor , Varga Zoltán , Kalocsai Renátó , Botta-Dukát Zoltán , Czúcz Bálint , Bede-Fazekas Ákos
Published: April 2022

Phacelia tanacetifolia, an excellent cover, green manure and honey crop is now widely cultivated throughout the world. One of its principal European seed production regions is north-western Hungary, where the recent withdrawal of a potent herbicide, linuron, created a new challenge for many growers. The goal of this study is to identify the main factors determining weed species composition in the phacelia fields of the region and to assess the efficiency of tine harrow and clopyralid herbicide in reducing weed abundance and biomass. We carried out a series of weed surveys across the study region following a two-level design: (i) we estimated the cover of all weed species in 205 fields (broad-scale survey, BS); and (ii) in 22 of these fields, we provided more precise biomass measurements (counting the individuals and measuring the dry weights of all weed species) in microplots samples (fine-scale survey; FS). To characterize the fields, 34 background variables were also collected for all of the studied fields. In both investigations, Chenopodium album was by far the most abundant weed. Within the BS, using a minimal adequate model containing 11 terms with significant net effects, 20.93% of the total variation in weed species data could be explained. The variation in species composition was determined by environmental factors (soil pH, clay and K; precipitation and temperature), non-chemical management variables (crop cover, preceding crop, irrigation and tillage system) and herbicides (linuron and clopyralid). Variation partitioning demonstrated the dominance of environmental and cultural components in shaping the weed species composition. Although the effect of mechanical treatments was most likely masked in the BS by the soil properties, our FS suggests that tine harrow could efficiently decrease the total number and biomass of weeds and can be a useful tool in the phacelia management of the future.
Uncovering the Industrial Potentials of Lemongrass Essential Oil as a Food Preservative: A Review
Authors: Faheem Fatima , Liu Zhi Wei , Rabail Roshina , Haq Iahtisham-Ul , Gul Maryam , Bryła Marcin , Roszko Marek , Kieliszek Marek , Din Ahmad , Aadil Rana Muhammad
Published: April 2022

The food industry is growing vastly, with an increasing number of food products and the demand of consumers to have safe and pathogen-free food with an extended shelf life for consumption. It is critical to have food safe from pathogenic bacteria, fungi, and unpleasant odors or tastes so that the food may not cause any health risks to consumers. Currently, the direction of food industry has been shifting from synthetically produced preservatives to natural preservatives to lower the unnecessary chemical burden on health. Many new technologies are working on natural prevention tools against food degradation. Lemongrass is one such natural preservative that possesses significant antimicrobial and antioxidant activity. The essential oil of lemongrass contains a series of terpenes that are responsible for these activities. These properties make lemongrass acceptable in the food industry and may fulfill consumer demands. This article provides detailed information about the role of lemongrass and its essential oil in food preservation. The outcomes of the research on lemongrass offer room for its new technological applications in food preservation.
A Genotype–Phenotype Analysis of Glutathione Peroxidase 4 in Human Atrial Myocardium and Its Association with Postoperative Atrial Fibrillation
Authors: Berdaweel Islam A. , Hart Alexander A. , Jatis Andrew J. , Karlan Nathan , Akhter Shahab A. , Gaine Marie E. , Smith Ryan M. , Anderson Ethan J.
Published: April 2022

Heterogeneity in the incidence of postoperative atrial fibrillation (POAF) following heart surgery implies that underlying genetic and/or physiological factors impart a higher risk of this complication to certain patients. Glutathione peroxidase-4 (GPx4) is a vital selenoenzyme responsible for neutralizing lipid peroxides, mediators of oxidative stress known to contribute to postoperative arrhythmogenesis. Here, we sought to determine whether GPX4 single nucleotide variants are associated with POAF, and whether any of these variants are linked with altered GPX4 enzyme content or activity in myocardial tissue. Sequencing analysis was performed across the GPX4 coding region within chromosome 19 from a cohort of patients (N = 189) undergoing elective coronary artery bypass graft (−/+ valve) surgery. GPx4 enzyme content and activity were also analyzed in matching samples of atrial myocardium from these patients. Incidence of POAF was 25% in this cohort. Five GPX4 variants were associated with POAF risk (permutated p ≤ 0.05), and eight variants associated with altered myocardial GPx4 content and activity (p < 0.05). One of these variants (rs713041) is a well-known modifier of cardiovascular disease risk. Collectively, these findings suggest GPX4 variants are potential risk modifiers and/or predictors of POAF. Moreover, they illustrate a genotype–phenotype link with this selenoenzyme, which will inform future mechanistic studies.
An Intelligent Cluster-Based Routing Scheme in 5G Flying Ad Hoc Networks
Authors: Khan Muhammad Fahad , Yau Kok-Lim Alvin , Ling Mee Hong , Imran Muhammad Ali , Chong Yung-Wey
Published: April 2022

Flying ad hoc network (FANET) is an application of 5G access network, which consists of unmanned aerial vehicles or flying nodes with scarce resources and high mobility rates. This paper proposes a deep Q-network (DQN)-based vertical routing scheme to select routes with higher residual energy levels and lower mobility rates across network planes (i.e., macro-plane, pico-plane, and femto-plane), which has not been investigated in the literature. The main motivation behind this work is to address frequent link disconnections and network partitions in order to enhance network performance. The 5G access network has a central controller (CC) and distributed controllers (DCs) in different network planes. The proposed scheme is a hybrid approach that allows CC and DCs to exchange information among themselves, and handle global and local information, respectively. The proposed scheme is suitable for highly dynamic ad hoc FANETs, and it enables data communication between UAVs in various applications, such as monitoring and performing surveillance of borders, and targeted-based operations (e.g., object tracking). Vertical routing is performed over a clustered network, in which clusters are formed across different network planes to provide inter-plane and inter-cluster communications. This helps to offload data traffic across different network planes to enhance network lifetime. Compared to the traditional reinforcement learning approach, the proposed DQN-based vertical routing scheme has shown to increase network lifetime by up to 60%, reduce energy consumption by up to 20%, and reduce the rate of link breakages by up to 50%.