bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Sensitivity of Binary/Ternary Locally Resonant Porous Phononic Crystal Sensors for Sulfuric Acid Detection: A New Class of Fluidic-Based Biosensors
Authors: Aliqab Khaled , Elsayed Hussein A. , Alsharari Meshari , Armghan Ammar , Ahmed Ashour M. , Mehaney Ahmed
Published: June 2023

This research presented a comprehensive study of a one-dimensional (1D) porous silicon phononic crystal design as a novel fluidic sensor. The proposed sensor is designed to detect sulfuric acid (H2SO4) within a narrow concentration range of 0–15%. Sulfuric acid is a mineral acid extensively utilized in various physical, chemical, and industrial applications. Undoubtedly, its concentration, particularly at lower levels, plays a pivotal role in these applications. Hence, there is an urgent demand for a highly accurate and sensitive tool to monitor even the slightest changes in its concentration, which is crucial for researchers. Herein, we presented a novel study on the optimization of the phononic crystal (PnC) sensor. The optimization process involves a comparative strategy between binary and ternary PnCs, utilizing a multilayer stack comprising 1D porous silicon (PSi) layers. Additionally, a second comparison is conducted between conventional Bragg and local resonant PnCs to demonstrate the design with the highest sensitivity. Moreover, we determine the optimum values for the materials’ thickness and number of periods. The results revealed that the ternary local resonant PnC design with the configuration of {silicone rubber/[PSi1/PSi2/PSi3]N/silicone rubber} is the optimal sensor design. The sensor provided a super sensitivity of 2.30 × 107 Hz for a concentration change of just 2%. This exceptional sensitivity is attributed to the presence of local resonant modes within the band gap of PnCs. The temperature effects on the local resonant modes and sensor performance have also been considered. Furthermore, additional sensor performance parameters such as quality factor, figure of merit, detection limit, and damping rate have been calculated to demonstrate the effectiveness of the proposed liquid sensor. The transfer matrix method was utilized to compute the transmission spectra of the PnC, and Hashin’s expression was employed to manipulate the porous silicon media filled with sulfuric acid at various concentrations. Lastly, the proposed sensor can serve as an efficient tool for detecting acidic rain, contaminating freshwater, and assessing food and liquid quality, as well as monitoring other pharmaceutical products.
Non-Invasive and Label-Free On-Chip Impedance Monitoring of Heatstroke
Authors: Zhao Yueli , Fan Weihua , Liu Anwei , Pan Shihua , Xu Chongxiao , Peng Hailun , Yin Bingling , Wang Xiaodong , Dong Jianhua , Pan Zhiguo
Published: June 2023

Heatstroke (HS) is a life-threatening injury requiring neurocritical care which could lead to central nervous system dysfunction and severe multiple organ failure syndrome. The cell–cell adhesion and cell permeability are two key factors for characterizing HS. To investigate the process of HS, a biochip-based electrical model was proposed and applied to HS. During the process, the value of TEER is associated with cell permeability and CI which represents cell–cell adhesion decreases that are consistent with the reduction in cell–cell adhesion and cell permeability characterized by proteins (occludin, VE-Cadherin and ZO-1) and RNA level. The results imply that the model can be used to monitor the biological process and other biomedical applications.
Intensity-Based Camera Setup for Refractometric and Biomolecular Sensing with a Photonic Crystal Microfluidic Chip
Authors: Kraft Fabio Aldo , Lehmann Stefanie , Di Maria Carmela , Joksch Leonie , Fitschen-Östern Stefanie , Fuchs Sabine , Dell’Olio Francesco , Gerken Martina
Published: June 2023

Label-free sensing is a promising approach for point-of-care testing devices. Among optical transducers, photonic crystal slabs (PCSs) have positioned themselves as an inexpensive yet versatile platform for label-free biosensing. A spectral resonance shift is observed upon biomolecular binding to the functionalized surface. Commonly, a PCS is read out by a spectrometer. Alternatively, the spectral shift may be translated into an intensity change by tailoring the system response. Intensity-based camera setups (IBCS) are of interest as they mitigate the need for postprocessing, enable spatial sampling, and have moderate hardware requirements. However, they exhibit modest performance compared with spectrometric approaches. Here, we show an increase of the sensitivity and limit of detection (LOD) of an IBCS by employing a sharp-edged cut-off filter to optimize the system response. We report an increase of the LOD from (7.1 ± 1.3) × 10−4 RIU to (3.2 ± 0.7) × 10−5 RIU. We discuss the influence of the region of interest (ROI) size on the achievable LOD. We fabricated a biochip by combining a microfluidic and a PCS and demonstrated autonomous transport. We analyzed the performance via refractive index steps and the biosensing ability via diluted glutathione S-transferase (GST) antibodies (1:250). In addition, we illustrate the speed of detection and demonstrate the advantage of the additional spatial information by detecting streptavidin (2.9 µg/mL). Finally, we present the detection of immunoglobulin G (IgG) from whole blood as a possible basis for point-of-care devices.
Comparison of Physical and Mechanical Properties of Stone Aggregates and Their Use in the Structure of a Flexible Pavement, from Mines in Ecuador
Authors: López Alex , Mayacela Margarita , Chérrez Diego , Aldas Emilio , Contreras Luis Felipe
Published: June 2023

One of the reasons that cause premature deterioration of the wearing course is the quality of the materials that make up the flexible pavement structure of the road network in Ecuador. Therefore, there is a need to thoroughly study the stone materials, such as coarse aggregate and fine aggregate, which form the structure of the flexible pavement. The quality of these materials will determine the service life of the wearing course as well as the high or low cost of road construction. The main objective of this research was to determine the highest quality material based on its technical and economic characteristics. For this purpose, three main mines that supply stone materials in the country were selected: “Kumochi”, “Los Muelles”, and “Cantera El Salvador”. Several samples of fine aggregate and coarse aggregate were taken from these mines to conduct laboratory tests, including natural moisture content, Atterberg limits, gradation, modified Proctor, and relative density. The California Bearing Ratio (CBR) test was also performed to determine the load-bearing capacity of the samples. These data will be used in future investigations for the design of sustainable pavement structures. Additionally, physical and mechanical properties were determined through tests including aggregate soundness, resistance to abrasion, and the Los Angeles abrasion test to determine the percentage of fractured faces of the coarse aggregate. In conclusion, it was found that the material from the “El Salvador” mine has the best technical and economic characteristics for the formation of flexible pavement structures. However, the material from the “Kumochi” and “Los Muelles” mines met the standards of the international AASHTO norm. The final recommendation after conducting the research is that the aforementioned materials can be used not only for the formation of the pavement structure but also for the wearing course.
A Stochastic Earthquake Ground Motion Database and Its Application in Seismic Analysis of an RC Frame-Shear Wall Structure
Authors: Ding Yanqiong , Xu Yazhou , Ding Shuhang
Published: June 2023

A stochastic earthquake ground motion database comprising twelve groups of simulated ground motions was introduced. Ground motions were generated using the stochastic semi-physical model of earthquake ground motions, based on a cluster analysis of 7778 recorded earthquake ground motion. All twelve groups of simulated earthquake ground motions were validated through the probability density evolution method (PDEM) by comparing their time histories and response spectra. As an application of the proposed database, an 18-story reinforced concrete (RC) frame-shear wall structure was analyzed using one group of simulated earthquake ground motions. The probability densities of the top displacement of the structure were estimated using PDEM, highlighting the significant stochasticity of the structural response. The seismic reliability of the structure was assessed by evaluating the extreme value distribution of the story drift angle. The investigations indicate that the proposed stochastic earthquake ground motion database effectively captures the inherent stochasticity of ground motions. Moreover, it contributes to enhancing the efficiency of reliability assessments for structures.
Research on Loading Scheme for Large-Scale Model Tests of Super-Long-Span Arch Bridge
Authors: Fan Yonghui , Zhou Jianting , Luo Chao , Yang Jun , Xin Jingzhou , Wang Shaorui
Published: June 2023

A reasonable and efficient loading scheme is needed to guarantee the success of large-scale bridge tests. In this study, an array-type, self-balancing pulley-group loading system was designed based on the world’s largest spanning arch bridge using a 1:10 scale model test. Automatic statistics of the required load at each loading point were realized using ANSYS, and a load optimization algorithm for loading points at different construction stages was proposed. Tests were carried out separately for the loading system using a single set of pulley groups and an array-type pulley group. Finite element models of the model bridge and the original bridge were established separately using ANSYS, and the stress results of different components during different construction stages of the main arch ring were compared. The research results show the following: (1) The load magnification factor of the single-pulley-group loading device is approximately 6.6 times, with a mechanical efficiency of 94.26%. (2) In the array-type loading system, the actual load at each loading point can reach the design value. The self-balancing characteristic of this system can eliminate the impact of vertical deformation of the structure on loading accuracy, verifying the reliability of the system. (3) The simulation results of the original bridge and the model bridge coincide well, and the stress of each component during the construction process has the same trend. At key construction stages, the maximum relative errors of the stress results of the rigid steel frame and the concrete inside the pipe of the two bridges are 8.33% and 9.34%, respectively, and the maximum absolute error of the bottom plate concrete is 0.66 MPa, verifying the correctness of the counterweight-optimization method. The loading scheme proposed in this paper can provide a reference for the design of loading systems with the same type of scale model test.
CTHRC1 Induces Pancreatic Stellate Cells (PSCs) into Myofibroblast-like Cancer-Associated Fibroblasts (myCAFs)
Authors: Kang Min Kyung , Jiang Fen , Kim Ye Ji , Ryu Kyoungjin , Masamune Atsushi , Hamada Shin , Park Yun-Yong , Koh Sang Seok
Published: June 2023

[BACKGROUND] Collagen triple helix repeat containing-1 (CTHRC1) is a secreted protein that contributes to the progression of various cancers, including pancreatic cancer. The higher expression of CTHRC1 in tumor tissues is associated with poorer survival outcomes. However, its specific roles in tumor extracellular matrix (ECM) remodeling remain unclear. Our study aims to investigate the influences of CTHRC1 on pancreatic stellate cells (PSCs), a main source of ECM production in pancreatic cancer. [METHODS AND RESULTS] The analyses of the publicly available pancreatic cancer patient data revealed that CTHRC1 is mainly expressed in cancer stroma and highly correlated with ECM-related genes. An in vitro study showed that more than 40% of these genes can be upregulated by CTHRC1. CTHRC1 specifically activated PSC into myofibroblast-like cancer-associated fibroblasts (myCAFs), which are characterized by a significantly upregulated POSTN gene expression. Periostin (coded by the POSTN gene) has a central role in the CTHRC1–PSCs–cancer metastasis axis. Furthermore, CTHRC1 promoted pancreatic cancer cell proliferation through PSC activation to a greater extent than via direct stimulation. Proof-of-concept experiments showed that the long-term (4-week) inhibition of CTHRC1 led to significant tumor suppression and ECM reduction, and also resulted in an unexpected shift in the CAF subtype from myCAFs to inflammatory CAFs (iCAFs). [CONCLUSION] PSC activation was demonstrated to be the key molecular mechanism responsible for the tumor-promoting effects of CTHRC1, and CTHRC1 has a critical role in CAF subtype differentiation and tumor microenvironment (TME) remodeling. The inhibition of CTHRC1 as a therapeutic strategy for the treatment of pancreatic cancer warrants further investigation.
Post-Surgical Prognosis of Patients with Pineoblastoma: A Systematic Review and Individual Patient Data Analysis with Trends over Time
Authors: Nandoliya Khizar R. , Sadagopan Nishanth S. , Thirunavu Vineeth , Houskamp Ethan J. , Karras Constantine L. , Chaliparambil Rahul K. , Sriram Nikhil , Jamshidi Pouya , Raleigh David R. , Lukas Rimas V. , Magill Stephen T.
Published: June 2023

Most of the literature on pineoblastoma consists of case reports and single-institution series. The goal of this systematic review and individual patient data (IPD) analysis was to summarize the existing literature, identify factors associated with overall survival (OS), and provide a contemporary update on prognosis for patients with pineoblastoma. Forty-four studies were identified with 298 patients having IPD. Kaplan–Meier analyses were used to report survival outcomes based on age, tumor metastases, extent of resection (EOR), adjuvant therapy, and publication year. Cox regression was performed to identify independent predictors of time to mortality. Multivariable recursive partitioning analysis was used to identify the most important subgroups associated with mortality. Patients were classified based on publication year before and after the last systematic review on this topic (pre-2012 and 2012 onwards) and compared using univariate and multivariable analyses. This study demonstrates that EOR less-than-gross total resection, metastatic presentation, adjuvant chemotherapy without radiation, and tumor presentation in children less than three years old are associated with poorer prognosis. Since 2012, the 5-year actuarial OS has improved from 32.8% to 56.1%, which remained significant even after accounting for EOR, age, and adjuvant therapy. Pineoblastoma remains a severe rare disease, but survival outcomes are improving.
Influence of Laparoscopic Surgery on Cellular Immunity in Colorectal Cancer: A Systematic Review and Meta-Analysis
Authors: Bohne Annika , Grundler Elena , Knüttel Helge , Fürst Alois , Völkel Vinzenz
Published: June 2023

Colorectal cancer (CRC) is the third most common cancer worldwide. The main treatment options are laparoscopic (LS) and open surgery (OS), which might differ in their impact on the cellular immunity so indispensable for anti-infectious and antitumor defense. MEDLINE, Embase, Web of Science (SCI-EXPANDED), the Cochrane Library, Google Scholar, ClinicalTrials.gov, and ICTRP (WHO) were systematically searched for randomized controlled trials (RCTs) comparing cellular immunity in CRC patients of any stage between minimally invasive and open surgical resections. A random effects-weighted inverse variance meta-analysis was performed for cell counts of natural killer (NK) cells, white blood cells (WBCs), lymphocytes, CD4+ T cells, and the CD4+/CD8+ ratio. The RoB2 tool was used to assess the risk of bias. The meta-analysis was prospectively registered in PROSPERO (CRD42021264324). A total of 14 trials including 974 participants were assessed. The LS groups showed more favorable outcomes in eight trials, with lower inflammation and less immunosuppression as indicated by higher innate and adaptive cell counts, higher NK cell activity, and higher HLA-DR expression rates compared to OS, with only one study reporting lower WBCs after OS. The meta-analysis yielded significantly higher NK cell counts at postoperative day (POD)4 (weighted mean difference (WMD) 30.80 cells/µL [19.68; 41.92], p < 0.00001) and POD6–8 (WMD 45.08 cells/µL [35.95; 54.21], p < 0.00001). Although further research is required, LS is possibly associated with less suppression of cellular immunity and lower inflammation, indicating better preservation of cellular immunity.
The Highs and Lows of Memantine—An Autophagy and Mitophagy Inducing Agent That Protects Mitochondria
Authors: de Wet Sholto , Mangali Asandile , Batt Richard , Kriel Jurgen , Vahrmeijer Nicola , Niehaus Dana , Theart Rensu , Loos Ben
Published: June 2023

Memantine is an FDA-approved, non-competitive NMDA-receptor antagonist that has been shown to have mitochondrial protective effects, improve cell viability and enhance clearance of Aβ42 peptide. Currently, there are uncertainties regarding the precise molecular targets as well as the most favourable treatment concentrations of memantine. Here, we made use of an imaging-based approach to investigate the concentration-dependent effects of memantine on mitochondrial fission and fusion dynamics, autophagy and mitochondrial quality control using a neuronal model of CCCP-induced mitochondrial injury so as to better unpack how memantine aids in promoting neuronal health. GT1-7 murine hypothalamic cells were cultured under standard conditions, treated with a relatively high and low concentration (100 µM and 50 µM) of memantine for 48 h. Images were acquired using a Zeiss 780 PS1 platform. Utilising the mitochondrial event localiser (MEL), we demonstrated clear concentration-dependent effects of memantine causing a protective response to mitochondrial injury. Both concentrations maintained the mitochondrial network volume whilst the low concentration caused an increase in mitochondrial number as well as increased fission and fusion events following CCCP-induced injury. Additionally, we made use of a customised Python-based image processing and analysis pipeline to quantitatively assess memantine-dependent changes in the autophagosomal and lysosomal compartments. Our results revealed that memantine elicits a differential, concentration-dependent effect on autophagy pathway intermediates. Intriguingly, low but not high concentrations of memantine lead to the induction of mitophagy. Taken together, our findings have shown that memantine is able to protect the mitochondrial network by preserving its volume upon mitochondrial injury with high concentrations of memantine inducing macroautophagy, whereas low concentrations lead to the induction of mitophagy.
Novel Small Molecules in IBD: Current State and Future Perspectives
Authors: Jefremow André , Neurath Markus F.
Published: June 2023

Biologicals have dominated the therapeutic scenery in inflammatory bowel diseases (IBDs), namely ulcerative colitis (UC) and Crohn’s disease (CD), for the past 20 years. The development of tofacitinib was the starting point for an era of small molecules after the era of biologicals. These new agents may challenge the use of biological agents in the future. They share properties that appeal to both patients and physicians. Low production costs, a lack of immunogenicity, and ease of use are only some of their benefits. On the other hand, patients and their physicians must manage the potential side effects of small molecules such as JAK inhibitors or S1P1R modulators. Here, we present agents that have already entered the clinical routine and those that are still being investigated in clinical trials.
Synthesis of Selenium-Based Small Molecules Inspired by CNS-Targeting Psychotropic Drugs and Mediators
Authors: Ribaudo Giovanni , Zeppilli Davide , Ongaro Alberto , Bortoli Marco , Zagotto Giuseppe , Orian Laura
Published: June 2023

Due to its endogenously high oxygen consumption, the central nervous system (CNS) is vulnerable to oxidative stress conditions. Notably, the activity of several CNS-targeting compounds, such as antidepressant and hypnotic drugs, or endogenous mediators, such as melatonin, is indeed linked to their ability of mitigating oxidative stress. In this work, we report the synthesis of two organoselenium compounds of which the structure was inspired by CNS-targeting psychotropic drugs (zolpidem and fluoxetine) and an endogenous mediator (melatonin). The molecules were designed with the aim of combining the ROS-scavenging properties, which were already assessed for the parent compounds, with a secondary antioxidant action, a glutathione peroxidase (GPx) mimic role empowered by the presence of selenium. The compounds were obtained through a facile three-step synthesis and were predicted by computational tools to passively permeate through the blood–brain barrier and to efficiently bind to the GABA A receptor, the macromolecular target of zolpidem. Of note, the designed synthetic pathway enables the production of several other derivatives through minor modifications of the scheme, paving the way for structure–activity relationship studies.
Investigation of the Seasonal Variation in Fat Patterning of Ellisras Rural Children and Adolescents, in the Limpopo Province, South Africa
Authors: Mulaudzi Lusani , Mkhatshwa Thandiwe N. , Makhubedu Mankopodi M. , Matshipi Moloko , Lebelo Sogolo L. , Monyeki Kotsedi D.
Published: June 2023

An increase in childhood obesity has become a global concern since childhood obesity often leads to adulthood obesity. This study aimed to investigate whether there is seasonal variation in fat patterning, and whether fat patterning is associated with seasons among the Ellisras population aged 5–15 years. A total of 1986 children and adolescents (1034 boys and 952 girls) aged 5–15 enrolled in this cross-sectional study. Skinfold measurements were obtained using standard procedures. Obesity prevalence was determined using frequency analysis. The correlation between obesity and two seasons was tested using multinomial regression analysis. The results showed that girls aged 11–15 years had significantly (p ≤ 0.03) larger median fat variables (triceps, biceps, and suprailiac) in spring compared to autumn. The prevalence of obesity ranged from 3–11% with boys being more obese (4–11%) than girls (3–7%) aged 5–15. Regression analysis showed a negative significant (p ≤ 0.001) correlation between autumn and obesity by the subscapular/(subscapular+triceps) (SST) ratio, both unadjusted −2.991 (95%CI −3.233:−2.803) and adjusted −2.897 (95% CI −4.331:−1.629). The findings of the study showed that there was seasonal variation in fat patterning among boys and girls in the Ellisras population and that fat patterning is associated with seasons.
Insight into Asymmetry in the Impact of Different Types of ENSO on the NAO
Authors: Zhang Peng , Wu Zhiwei
Published: June 2023

Understanding the influence of the El Niño–Southern Oscillation (ENSO) on the North Atlantic Oscillation (NAO) is of critical significance for seasonal prediction. The present study found that both Niño3.4 sea surface temperature anomaly (SSTA) intensity and east-west gradient in the mid-low latitude Pacific determine the linkage between ENSO and the NAO. Based on Niño3.4 SSTA intensity and the east-west gradient, ENSO events are classified into three types: strong intensity, weak intensity-strong gradient (WSG), and equatorial ENSOs. Note that the former two types are usually concurrent with a strong zonal SSTA gradient. In contrast, equatorial ENSO is often associated with weak intensity-weak gradient SSTAs confined in the equatorial Pacific. The anomalous circulation patterns in response to the three types of ENSO exhibit asymmetric features over the North Atlantic. The WSG-El Niño associated circulation anomaly resembles a negative NAO-like pattern, yet the strong and equatorial El Niño associated circulation anomalies show a neutral-NAO pattern. For La Niña events, their impact on the NAO mainly depends on the cold SSTA position rather than their intensity. The strong and WSG-La Niña associated negative SSTAs are centered in the equatorial-central Pacific and favor a steady positive NAO-like anomaly. The cold SSTA center of equatorial La Niña shifts to the equatorial-eastern Pacific and cannot profoundly influence the North Atlantic climate. The physical mechanisms are also investigated with a general circulation model.
Theoretical Study of Thermoelectric Properties of a Single Molecule of Diphenyl-Ether
Authors: Toscano-Negrette Rafael G. , León-González José C. , Vinasco Juan A. , Ojeda Silva Judith Helena , Morales Alvaro L. , Duque Carlos A.
Published: June 2023

Taking into consideration the research that has been conducted on the optical and electrical properties of molecular systems, especially the good thermoelectric energy conversion at a nanometric scale that such systems have presented, here we present a new alternative by using a particular diphenyl-ether molecule as a functional device. Such a molecular system is modeled as a planar segment coupled to two electrodes in the first-neighbor approximation within a tight-binding Hamiltonian. We study the electrical and thermal properties of diphenyl-ether molecules such as the electric current, electrical and thermal conductance, Seebeck coefficient, and figure of merit, in the strong and weak coupling regimes, considering different structural configurations and variations with temperature. Our results could be valuable for laboratory applications and/or verification since we characterize the diphenyl-ether molecule as a semiconductor device for different structural models.
In Vitro Assessment of the Synergistic Effect of Aspirin and 5-Fluorouracil in Colorectal Adenocarcinoma Cells
Authors: Susan Monica , Macasoi Ioana , Pinzaru Iulia , Dehelean Cristina , Ilia Iosif , Susan Razvan , Ionita Ioana
Published: June 2023

Although remarkable progress has been made, colorectal cancer remains a significant global health issue. One of the most challenging aspects of cancer treatment is the resistance of tumor cells to classical chemotherapy. Conventional therapy for colorectal cancer often involves the use of 5-fluorouracil as a chemotherapeutic agent. Aspirin, a drug used primarily to prevent cardiovascular complications, became a focus of attention due to its potential use as an antitumor agent. The purpose of the study was to evaluate the potential synergistic cytotoxic effects of aspirin and 5-fluorouracil on colorectal adenocarcinoma cells. The viability of cells, the impact on the morphology and nuclei of cells, the potential antimigratory effect, and the impact on the expression of the major genes associated with cell apoptosis (Bcl-2, Bax, Bad), as well as caspases 3 and 8, were evaluated. The results indicated that the two compounds exerted a synergistic effect, causing a reduction in cell viability accompanied by changes characteristic of the apoptosis process—the condensation of nuclei and the reorganization of actin filaments in cells, the reduction in the expression of the Bcl-2 gene, and the increase in the expression of Bax and Bad genes, along with caspases 3 and 8. Considering all these findings, it appears that aspirin may be investigated in depth in order to be used in conjunction with 5-fluorouracil to increase antitumor activity.
Latitudinal Difference in the Condition Factor of Two Loliginidae Squid (Beka Squid and Indian Squid) in China Seas
Authors: Guo Jianzhong , Zhang Chi , Li Zhixin , Liu Dan , Tian Yongjun
Published: June 2023

Cephalopod fisheries in the China Seas have witnessed an increasing trend in the catches of coastal cephalopods since the 1990s, with Loliginidae squid emerging as the main commercial target species. However, climate change and overfishing have led to a dramatic reduction in Loliginidae squid resources, highlighting the need to improve monitoring, protection, and management of this species. The Loligo beka and Uroteuthis duvaucelii are widely distributed along the coastal areas of the China Seas, and have commercial and ecological importance. Despite having overlapping distributions, similar life histories, and a strong dependence on the marine environment, there is limited knowledge about their growth and responses to environmental changes, hindering the effective management of their resources. In this study, we investigated the interspecies and intra-species differences in condition factor and their responses to temperature changes by analyzing data collected from wide coastal areas of the China Seas from June 2019 to November 2020. The findings showed that both species exhibited allometric growth and reproduced throughout the year, with two main breeding peaks. There were significant monthly variations and latitude differences in the intra-species growth, with a higher proportion of small-sized individuals (between 5 and 10 g for L. beka and between 10 and 20 g for U. duvaucelii) in low-latitude waters. The latitudinal differences in body weight and distribution between and within the two species were mainly due to natural habitats, especially temperature. Our mixed effect model results demonstrated that both species’ body weight increased with increasing temperature, suggesting that Loliginidae squid have significant environmental adaptability and can be used as an indicator species for studying environmental changes in the China Seas. These findings have significant implications for understanding the population dynamics, species development, and regionally specific management of Loliginidae squid fisheries in the China Seas.
Optimal Domain-Partitioning Algorithm for Real-Life Transportation Networks and Finite Element Meshes
Authors: Bhagatji Jimesh , Asundi Sharanabasaweshwara , Thompson Eric , Nguyen Duc T.
Published: June 2023

For large-scale engineering problems, it has been generally accepted that domain-partitioning algorithms are highly desirable for general-purpose finite element analysis (FEA). This paper presents a heuristic numerical algorithm that can efficiently partition any transportation network (or any finite element mesh) into a specified number of subdomains (usually depending on the number of parallel processors available on a computer), which will result in “minimising the total number of system BOUNDARY nodes” (as a primary criterion) and achieve “balancing work loads” amongst the subdomains (as a secondary criterion). The proposed seven-step heuristic algorithm (with enhancement features) is based on engineering common sense and observation. This current work has the following novelty features: (i) complicated graph theories that are NOT needed and (ii) unified treatments of transportation networks (using line elements) and finite element (FE) meshes (using triangular, tetrahedral, and brick elements) that can be performed through transforming the original network (or FE mesh) into a pseudo-transportation network which only uses line elements. Several examples, including real-life transportation networks and finite element meshes (using triangular/brick/tetrahedral elements) are used (under MATLAB computer environments) to explain, validate and compare the proposed algorithm’s performance with the popular METIS software.
Expression of IL-21 and IL-33 in Intestinal Mucosa of Inflammatory Bowel Disease: An Immunohistochemical Study
Authors: Toskas Alexandros , Milias Stefanos , Delis Georgios , Meditskou Soultana , Sioga Antonia , Papamitsou Theodora
Published: June 2023

Interleukins are considered to be potential therapeutic targets that can alter the prognosis and disease progression of IBD. IL-21 has proven to be involved in effector Th1, Th2 and Th17 responses. Similarly, IL-33, a newly identified cytokine, has been shown to control the Th1 effector response and the action of the colonic Tregs in animal models of colitis and patients with IBD. In this retrospective study, we have studied the expression of these interleukins, using immunohistochemistry, in 121 patients with moderate to severe IBD before and after treatment with biologics. The results were statistically processed using SPSSTM. Increased IL-21 expression was found in the UC and CD groups versus the controls. The IL-33 expression was found to be increased in the post-treatment UC and CD groups, suggesting a protective role of this interleukin against bowel inflammation. The IL-33 expression post-treatment was reversely correlated with the activity index score in CD patients, suggesting a better response to treatment in patients with higher IL-33 mucosa levels. This is the first immunohistochemical study of the expression of those interleukins in bowel mucosa before and after treatment with biologics. These data support a possibly promising future use of these interleukins as biomarkers of severe disease and response to treatment and as potential therapeutic targets for novel monoclonal antibodies.
How to Evaluate Fibrosis in IBD?
Authors: Tavares de Sousa Helena , Magro Fernando
Published: June 2023

In this review, we will describe the importance of fibrosis in inflammatory bowel disease (IBD) by discussing its distinct impact on Crohn’s disease (CD) and ulcerative colitis (UC) through their translation to histopathology. We will address the existing knowledge on the correlation between inflammation and fibrosis and the still not fully explained inflammation-independent fibrogenesis. Finally, we will compile and discuss the recent advances in the noninvasive assessment of intestinal fibrosis, including imaging and biomarkers. Based on the available data, none of the available cross-sectional imaging (CSI) techniques has proved to be capable of measuring CD fibrosis accurately, with MRE showing the most promising performance along with elastography. Very recent research with radiomics showed encouraging results, but further validation with reliable radiomic biomarkers is warranted. Despite the interesting results with micro-RNAs, further advances on the topic of fibrosis biomarkers depend on the development of robust clinical trials based on solid and validated endpoints. We conclude that it seems very likely that radiomics and AI will participate in the future non-invasive fibrosis assessment by CSI techniques in IBD. However, as of today, surgical pathology remains the gold standard for the diagnosis and quantification of intestinal fibrosis in IBD.
Semaphorin 4D Induces Vasculogenic Differentiation of Dental Pulp Stem Cells
Authors: Al Turkestani Najla , Zhang Zhaocheng , Nör Jacques Eduardo
Published: June 2023

This work aimed to evaluate the effect of Semaphorin 4D (SEMA4D) signaling through Plexin B1 on the vasculogenic differentiation of dental pulp stem cells. We assessed the protein expression of SEMA4D and Plexin B1 in dental pulp stem cells (DPSC) from permanent human teeth and stem cells from human exfoliated deciduous (SHED) teeth using Western blots. Their expression in human dental pulp tissues and DPSC-engineered dental pulps was determined using immunofluorescence. We then exposed dental pulp stem cells to recombinant human SEMA4D (rhSEMA4D), evaluated the expression of endothelial cell differentiation markers, and assessed the vasculogenic potential of rhSEMA4D using an in vitro sprouting assay. Lastly, Plexin B1 was silenced to ascertain its role in SEMA4D-mediated vasculogenic differentiation. We found that SEMA4D and Plexin B1 are expressed in DPSC, SHED, and human dental pulp tissues. rhSEMA4D (25–100 ng/mL) induced the expression of endothelial markers, i.e., vascular endothelial growth factor receptor (VEGFR)-2, cluster of differentiation (CD)-31, and tyrosine kinase with immunoglobulin-like and EGF-like domains (Tie)-2, in dental pulp stem cells and promoted capillary-like sprouting in vitro (p < 0.05). Furthermore, Plexin B1 silencing abrogated the vasculogenic differentiation of dental pulp stem cells and significantly inhibited capillary sprouting upon exposure to rhSEMA4D. Collectively, these data provide evidence that SEMA4D induces vasculogenic differentiation of dental pulp stem cells through Plexin B1 signaling.
Do Increased Tax Base and Reductions in the Underground Economy Compensate for Lost Tax Revenue Following a Tax Reduction Policy? Evidence from Italy 1982 to 2006
Authors: Orsi Renzo , Seip Knut Lehre
Published: June 2023

We here examine the frequent claim that an increase in the tax base and a decrease in tax evasion will compensate for a loss in tax revenues caused by a lower tax level. Using a unique data set for the estimated underground economy in Italy from 1982 to 2006, we found that a loss in tax revenues equivalent to 1% of the GDP would be partly compensated by an increase in GDP of 0.55%. The compensation would come from 0.31% of the GDP increase and from 0.24% of the reductions in the underground economy. These results apply to an economy with a high tax level (>32%) and a high underground economy (≥25%). Applying a high-resolution lead–lag method to the data, we ensured that tax changes were leading the GDP and, thus, a potential cause for changes in the GDP.
An Electronic Microsaccade Circuit with Charge-Balanced Stimulation and Flicker Vision Prevention for an Artificial Eyeball System
Authors: Liang Yaogan , Nakamura Kohei , Du Bang , Wang Shengwei , Inoue Bunta , Aruga Yuta , Kino Hisashi , Fukushima Takafumi , Kiyoyama Koji , Tanaka Tetsu
Published: June 2023

This paper presents the first circuit that enables microsaccade function in an artificial eyeball system. Currently, the artificial eyeball is receiving increasing development in vision restoration. The main challenge is that the human eye is born with microsaccade that helps refresh vision, avoiding perception fading while the gaze is fixed for a long period, and without microsaccade, high-quality vision restoration is difficult. The proposed electronic microsaccade (E-μSaccade) circuit addresses the issue, and it is intrinsically safe because only charge-balanced stimulus pulses are allowed for stimulation. The E-μSaccade circuit adopts light-to-frequency modulation; due to the circuit’s leakage and dark current of light-sensitive elements, stimulus pulses of a frequency lower than tens of Hz occur, which is the cause of flickering vision. A flicker vision prevention (FVP) circuit is proposed to mitigate the issue. The proposed circuits are designed in a 0.18 μm standard CMOS process. The simulation and measurement results show that the E-μSaccade circuit helps refresh the stimulation pattern and blocks the low-frequency output.
Near-Field Coupling Effect Analysis of SMD Inductor Using 3D-EM Model
Authors: Choi Gyeong Ryun , Kim HyongJoo , Hong Yonggi , Hwang Joosung , Kim Euihyuk , Nah Wansoo
Published: June 2023

In this paper, we propose a methodology for analyzing the near-field coupling between two surface mount device (SMD) inductors using a 3-dimensional electromagnetic (3D-EM) model. To develop the 3D-EM model, we first constitute the equivalent circuit of the SMD inductor from the measured impedance and derive the loss tangent using circuit parameters. Secondly, the loss tangent using damped harmonic oscillator model is introduced to extract the effective permeability of core magnetic material in the SMD inductor. The optimization algorithm is used to compare the two loss tangents. Then the effective permeability is used in the magnetic material for the 3D-EM modeling of the SMD inductor. The validity of the proposed 3D-EM model is confirmed by comparing the impedance and S-parameters obtained from both measured and EM-simulated values for the two near-field coupled SMD inductors. Finally, the near-field coupling effects between the two adjacent SMD inductors are visualized in terms of coupling path visualization (CPV) using the proposed 3D-EM model, which demonstrates its usefulness for near-field coupling analysis.
A High Performance Reconfigurable Hardware Architecture for Lightweight Convolutional Neural Network
Authors: An Fubang , Wang Lingli , Zhou Xuegong
Published: June 2023

Since the lightweight convolutional neural network EfficientNet was proposed by Google in 2019, the series of models have quickly become very popular due to their superior performance with a small number of parameters. However, the existing convolutional neural network hardware accelerators for EfficientNet still have much room to improve the performance of the depthwise convolution, squeeze-and-excitation module and nonlinear activation functions. In this paper, we first design a reconfigurable register array and computational kernel to accelerate the depthwise convolution. Next, we propose a vector unit to implement the nonlinear activation functions and the scale operation. An exchangeable-sequence dual-computational kernel architecture is proposed to improve the performance and the utilization. In addition, the memory architectures are designed to complete the hardware accelerator for the above computing architecture. Finally, in order to evaluate the performance of the hardware accelerator, the accelerator is implemented based on Xilinx XCVU37P. The results show that the proposed accelerator can work at the main system clock frequency of 300 MHz with the DSP kernel at 600 MHz. The performance of EfficientNet-B3 in our architecture can reach 69.50 FPS and 255.22 GOPS. Compared with the latest EfficientNet-B3 accelerator, which uses the same FPGA development board, the accelerator proposed in this paper can achieve a 1.28-fold improvement of single-core performance and 1.38-fold improvement of performance of each DSP.
Design and Comparative Analysis of an Ultra-Highly Efficient, Compact Half-Bridge LLC Resonant GaN Converter for Low-Power Applications
Authors: Faizan Muhammad , Wang Xiaolei , Yousaf Muhammad Zain
Published: June 2023

For low-power applications, this paper presents the development and design of a compact and ultra-highly efficient half-bridge LLC resonant converter. By using Galium Nitride (GaN) devices and high-efficient magnetics, the efficiency and power density of resonant converters can be improved. Compared to Silicon MOSFETs, GaN high-electron-mobility transistors (GaN HEMT) have a lower output capacitance and gate charge, resulting in lower driving loss and shorter dead times. Consequently, the proposed LLC converter based on GaN devices has excellent performance characteristics such as ultra-high efficiency, low switching losses, compact size, high voltage endurance, high operating temperature and high operating frequency. Furthermore, the proposed resonant converter features soft switching properties that ensure that the switches and diodes on the primary side are always switched at zero voltage and current. By doing so, LLC resonant converter switching losses are significantly reduced by up to 3.1%, and an overall efficiency of 98.5% is achieved. The LLC resonant converter design with GaN HEMT has great advantages over Si MOSFET solution regarding efficiency, overall losses, switching loose and power factor correction. A 240 W, 240 V to 60 V half-bridge GaN HEMT LLC resonant converter is simulated with a switching frequency of 75 KHz, along with the comparative analysis of the Si metal oxide semiconductor field effect transistor (MOSFET) solution. Moreover, the design and analysis of highly efficient magnetics with a power factor of 0.99 at full load is presented. A 240-Watt single stage LED driver with power factor correction is also designed to verify and compare the performance of proposed LLC resonant converter.
Influence of Calcination Temperature on Electrochemical Properties of Perovskite Oxide Nanofiber Catalysts
Authors: Park Ji-Woo , Shin Jeeyoung , Ju Young-Wan
Published: June 2023

Energy conversion and storage systems have recently attracted significant attention owing to increasing environmental and energy problems. However, the slow kinetics of the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER) render their commercialization difficult. Many studies are being conducted to replace precious metal catalysts that have high activity for the ORR and OER but have scarcity and low stability. In this study, Sm0.5Sr0.5CoO3 (SSC) nanofibers were fabricated via the electrospinning of non-precious metal-based perovskite oxides to replace precious metal catalysts. In addition, the properties and electrochemical performance of SSC fibers synthesized at different calcination temperatures were evaluated. The small crystallite size, high specific surface area, abundant oxygen vacancies, and high ORR/OER activity suggest that SSC800 hollow fibers are optimal bifunctional catalysts.
Modeling of the Temperature Regimes in a Layered Bimetallic Plate under Short-Term Induction Heating
Authors: Musii Roman , Pukach Petro , Melnyk Nataliia , Vovk Myroslava , Šlahor L’udomír
Published: June 2023

A mathematical model for determining the temperature field of a bimetallic plate with plane-parallel boundaries during short-term induction heating by a non-stationary electromagnetic field is proposed. Initial boundary value problems for determining the parameters of a non-stationary electromagnetic field and temperature are formulated. The temperature and the component of the magnetic field intensity vector that is tangential to the plate base were selected as defining functions. We used an approximation of the defining functions in each layer of the plate with quadratic polynomials by the thickness coordinate and Laplace transform of the integral over time. General solutions to the formulated problems under uniform non-stationary electromagnetic action were obtained. Based on them, the temperature during short-term induction heating by a non-stationary electromagnetic field was numerically analyzed depending on its amplitude-frequency parameters and duration.
Optimization of Mathematical Function-Shaped Fracture Distribution Patterns for Multi-Stage Fractured Horizontal Wells
Authors: Zou Yi , Zhou Desheng , Ma Xianlin , Jie Yenan , Wang Xiaoxiang , Liu Hongxia
Published: June 2023

A conventional oil and gas well does not have a natural production capacity, which necessitates a hydraulic fracturing operation. The effectiveness of the fracturing directly impacts the economic benefit of a single well. Among the various parameters, including fracture spacing, fracture width, and conductivity, fracture half-length is one of the main influencing factors on the productivity of horizontal wells. For conventional homogeneous reservoirs, research mainly focuses on fracture patterns with equal fracture lengths. However, in actual production processes, due to mutual interference and the superimposition of drainage areas between fractures, the production distribution of each fracture is non-uniform. Typical fracture distribution patterns mainly include uniform, staggered, dumbbell, and spindle. While many believe that the dumbbell-shaped fracture distribution pattern has the best effect, there has been no quantitative study on the length of each fracture under the dumbbell-shaped pattern. Based on this, this paper proposes a modeling approach for function-shaped fracture distribution that takes advantage of the high production of edge fractures and the low output of middle fractures in horizontal wells. The influence of this approach on production capacity is studied. Constant, linear, and parabolic functions are used to establish the relationship between fracture position and fracture half-length, optimizing the fracture distribution function to achieve the best production effect. This method can guide the horizontal well fracture distribution in the block to maximize productivity. The results show that the parabolic function-shaped model is better than the linear function-shaped model and the constant function-shaped model is the least effective. The research presented in this paper offers a new idea for optimizing on-site fracturing plans. It utilizes mathematical expressions to describe the parameters that affect productivity, which provides valuable guidance for designing multi-stage fractured horizontal wells in the field. In the future, this research will be extended by exploring the optimal fracture distribution function under different formation conditions.