bluuebunny/crossref_metadata_2025 huggingface dataset:
β-Ga2O3 Thin Films via an Inorganic Sol–Gel Spin Coating: Preparation and Characterization
Authors: Zhang Hai , Niu Dingyuan , Yang Junbiao , Zhang Xiaoyang , Zhu Jun , Li Wencai
Published: February 2025

β-Ga2O3 holds significant promise for use in ultraviolet (UV) detectors and high-power devices due to its ultra-wide bandgap. However, the cost-effective preparation of large-area thin films remains challenging. In this study, β-Ga2O3 thin films are prepared using an inorganic solution reaction spin-coating method followed by post-annealing. The structures, surface morphologies, and optical properties of the films are then characterized using X-ray diffraction, scanning electron microscopy, and ultraviolet–visible spectrophotometry. A low-cost Ga metal was used to produce NH4Ga(SO4)2, which was then converted into a precursor solution and spin-coated onto sapphire and quartz substrates. Ten cycles of spin coating produced smoother films, although higher annealing temperatures induced more cracks. The films on the (0001) sapphire subjected to spin-coating and preheating processes that were repeated for ten cycles, followed by annealing at 800 °C, had a preferred orientation in the [–201] direction. All the films showed high transmittances of 85% in ultraviolet–visible light with wavelengths above 400 nm. The films on the (0001) sapphire substrate that were annealed at 800 °C and 1000 °C exhibited bandgaps of 4.8 and 4.98 eV, respectively. The sapphire substrates demonstrated a superior compatibility for high-quality Ga2O3 film fabrication compared to quartz. This method offers a cost-effective and efficient approach for producing high-quality β-Ga2O3 films on high-temperature-resistant substrates with promising potential for optoelectronic applications.
Effects of Foliar Application of Copper and Gold Nanoparticles on Petroselinum crispum (Mill.)
Authors: Peshkova Alexandra , Zinicovscaia Inga , Rudi Ludmila , Chiriac Tatiana , Yushin Nikita , Cepoi Liliana
Published: February 2025

The unintentional release of nanoparticles in the atmosphere and their targeted application to improve plant productivity requires detailed study. The translocation features of copper and gold nanoparticles applied by spraying in the concentration range of 1–100 mg/L in Petroselinum crispum (Mill.) tissues during a 10-day experiment were investigated. Atomic absorption spectrometry and inductively coupled plasma atomic emission spectroscopy showed that copper and gold nanoparticles applied to the leaves’ surface could accumulate in plant organs. A dose-dependent increase in the content of copper and gold in the aerial parts of parsley was revealed. The content of copper in leaves treated with nanoparticles was 1–2.3 times higher than the control, while the content of gold exceeded control values 2–116 times. The effect of nanoparticles on plants’ biochemical composition was assessed. The antioxidant tests showed an ambiguous response at exposure to metal nanoparticles. Copper nanoparticles at the applied concentration consistently reduced both chlorophyll and carotenoid content. Gold nanoparticles enhanced the chlorophyll and carotenoid level at low concentrations (1 mg/L) and significantly inhibited it at higher concentrations. The parsley exposed to nano-copper remained safe for human consumption, but parsley containing more than 14.9 mg/kg of gold may adversely affect human health.
Polyphenol-Based Prevention and Treatment of Cancer Through Epigenetic and Combinatorial Mechanisms
Authors: Singaravelan Neha , Tollefsbol Trygve O.
Published: February 2025

Polyphenols have been shown to be utilized as an effective treatment for cancer by acting as a DNMT or HDAC inhibitor, reducing inflammatory processes, and causing cell cycle arrest. While there have been many studies demonstrating the anti-cancerous potential of individual polyphenols, there are limited studies on the combinatorial effects of polyphenols. This review focuses on how combinations of different polyphenols can be used as a chemotherapeutic treatment option for patients. Specifically, we examine the combinatorial effects of three commonly used polyphenols: curcumin, resveratrol, and epigallocatechin gallate. These combinations have been shown to induce apoptosis, prevent colony formation and migration, increase tumor suppression, reduce cell viability and angiogenesis, and create several epigenetic modifications. In addition, these anti-cancerous effects were synergistic and additive. Thus, these findings suggest that using different combinations of polyphenols at the appropriate concentrations can be used as a better and more efficacious treatment against cancer as compared to using polyphenols individually.
Five-Day Treatment with B. licheniformis Along with Classical Vancomycin Treatment Was Effective in Preserving Gut Microbiota in Patients with Clostridioides difficile Infection
Authors: Gweon Tae-Geun , Kang Sang-Bum , Na Soo-Young , Oh Dong Jun , Kim Sang Wook , Seo Geom Seog , Cho Joo Young
Published: February 2025

Background/Objectives: Clostridioides difficile infection (CDI) is an important nosocomial diarrheal disease. The benefits of the probiotic Bacillus licheniformis (B. licheniformis) in the preservation of intestinal microbiota have not been studied in patients with CDI to date. Therefore, we aimed to investigate the efficacy of B. licheniformis in preserving the intestinal microbiota in patients with CDI. Methods: A multicenter, randomized, placebo-controlled trial was carried out at six academic centers in Korea. Individuals diagnosed with mild to moderate CDI were included in this trial. CDI was treated with vancomycin 125 mg four times daily for two weeks. Along with vancomycin, B. licheniformis was administered for five days in this study, while a placebo was given to the placebo group. Microbiome analysis was performed before and five days after administering vancomycin and B. licheniformis or placebo, using 16S rRNA amplicon sequencing. Alpha and beta diversity was compared between the two groups. Results: A total of 35 participants were finally included in this study, with 16 in the study group and 19 in the placebo group. The alpha diversity was similar in both groups before CDI treatment. After five days of the administration of vancomycin and B. licheniformis or placebo, alpha diversity did not decrease in the study group (Chao1 index, p = 0.665; observed features, p = 0.692). In contrast, alpha diversity decreased in the placebo group (Chao1 index, p = 0.011; observed features, p = 0.011). Beta diversity did not differ between the two groups. Conclusions: The addition of B. licheniformis to vancomycin was effective in preserving gut microbiota in patients with CDI.
The Role of Dairy in Human Nutrition: Myths and Realities
Authors: Sanjulián Laura , Fernández-Rico Salvador , González-Rodríguez Nerea , Cepeda Alberto , Miranda José Manuel , Fente Cristina , Lamas Alexandre , Regal Patricia
Published: February 2025

Milk is a highly complex food that contains all the nutrients necessary for the development of mammalian offspring. For millennia, humans have included milk and milk products as major components of their diet. However, the effect of the consumption of dairy products on health has been a concern in recent years in terms of myths and realities. This review briefly describes the composition of bovine milk, the positive and negative effects that have been related to dairy products, and those aspects where the scientific evidence is still inconclusive. In addition to being nutritional, dairy products are a source of bioactive peptides, prebiotics and probiotics, fatty acids such as CLA, and fat globule membranes or have a protective effect against certain diseases. Negative effects include milk protein allergy or lactose intolerance. The effects of dairy products on certain cancers, such as prostate cancer, and their role in type II diabetes mellitus or weight gain are still inconclusive. Although the role of dairy products in cardiovascular risk is still inconclusive, recent meta-analyses have shown that dairy products may have a protective effect.
Bioaccessible Bread Melanoidins Modulate Oxidative Stress, Reduce Inflammation and Suppress Adhesion of Helicobacter pylori to Caco-2 Cells
Authors: Gerardi Gisela , Temiño Virginia , Salazar-Mardones Gonzalo , Díaz-Morales Noelia , Melero Beatriz , Bocigas Carolina , Muñiz Pilar , Rovira Jordi , Cavia-Saiz Mónica
Published: February 2025

Background/Objectives: Helicobacter pylori is a major contributor to gastric infections; it is prevalent in humans and associated with gastrointestinal diseases. In recent years, the increase in antimicrobial resistance has contributed to the need for alternative approaches, prompting interest in natural products with antimicrobial and antivirulence properties. This study investigated the effect of bioaccessible melanoidins from common and soft bread crust against H. pylori infection. Methods: Melanoidins were extracted using dead-end ultrafiltration, and bioaccessible fractions were obtained through in vitro digestion. The bactericidal effect of melanoidins was assessed at 2% and 4% concentrations over 24 and 48 h. The effect on H. pylori adhesion of 100 μg/mL and 200 μg/mL of gastric and intestinal bioaccessible fractions of melanoidins was evaluated in Caco-2 cells. Results: The bactericidal effect of melanoidins revealed significant efficacy, with a greater effect for soft bread melanoidins. The gastric fractions exhibited a higher inhibitory effect, which is crucial for gastric mucosa, the primary site of H. pylori infection. Both bioaccessible fractions showed anti-inflammatory and antioxidant effects against H. pylori-induced inflammation, particularly in the gastric fractions. This was evidenced by a reduction in interleukin-6 and interleukin-8 release and an enhancement in interleukin-10 release. The observed reduction in reactive oxygen species (ROS) and the maintenance of glutathione levels indicate an improved redox status. Conclusions: This study emphasizes the potential of melanoidins, especially from soft bread, as bioactive compounds against H. pylori, offering insights for future functional food development.
Psychobiotic Protection of Nutritional Supplements and Probiotics in Patients Undergoing Hemodialysis: A Randomized Trial
Authors: Climent Eric , Hevilla Francisco , Padial Marina , Barril-Cuadrado Guillermina , Blanca María , Jiménez-Salcedo Tamara , López-Picasso Maria , Nogueira-Pérez Ángel , Olveira Gabriel
Published: February 2025

Background/Objectives: The prevalence of depression and anxiety symptoms is remarkably high in malnourished individuals undergoing hemodialysis. The goal of this project was to evaluate the impact of administering an oral nutritional supplement combined with a probiotic blend on the microbiota, intestinal permeability, and depression symptoms in malnourished hemodialysis patients. Methods: With this aim, a randomized trial was conducted with three parallel groups: a control group with individualized diet, a supplement–placebo (SU-PL) group with oral nutritional supplementation (ONS), and a supplement–probiotic (SU-PR) group with ONS in conjunction with a probiotic blend. Blood and fecal samples were collected at basal time, and at 3 and 6 months. Several blood biomarkers, like zonulin, lipopolysaccharide-binding protein (LBP), lipopolysaccharide (LPS), and brain-derived neurotrophic factor (BDNF), were measured, and the fecal microbiome was sequenced with the Illumina platform. The Hospital Anxiety and Depression Scale (HADS) was used for the estimation of depression (HADS-D) and anxiety (HADS-A) symptoms, along with the standardized mental health index SF12-MH from the general health questionnaire SF-12. Results: The results showed that patients who consumed the probiotic blend maintained the LPS levels from their baseline readings and decreased their BDNF levels compared to the SU-PL or control groups. Moreover, a significant decrease in HADS-D scores (less depressive symptoms) and an increase in SF12-MH scores (higher quality of life) were found in that group in comparison to the other groups. The intervention produced an impact on the microbiome population, where the SU-PR group had reduced Akkermansia abundance with respect to the other groups, while their Acidaminococcus abundance decreased and their Barnesiella abundance increased with respect to the SU-PL group. Conclusions: Overall, the results indicate that the probiotic with the nutritional supplement could reduce the intestinal permeability biomarkers and improve depressive symptoms and quality of life in malnourished hemodialysis patients.
The Potential Role of Phytochemicals in Alzheimer’s Disease
Authors: Baranowska-Wójcik Ewa , Gajowniczek-Ałasa Dorota , Pawlikowska-Pawlęga Bożena , Szwajgier Dominik
Published: February 2025

Alzheimer’s disease (AD) is a neurodegenerative condition characterised by memory loss and cognitive disorders. The disease has been related to the presence of so-called senile plaques forming due to the buildup of amyloid β in the hippocampus. The AD therapies developed to date continue to prove insufficient, while long-term exposure to synthetic drugs tends to lead to serious side effects, which is why potential herbal treatments are generally preferable to conventional drug regimens and, as such, have been under considerable research scrutiny in recent years. There are a number of herbs, e.g., lavender Ginkgo biloba, that are already commonly employed in alleviating the symptoms of certain neurological disorders. In light of the above, the aim of the following paper is to discuss the importance of medicinal herbs, their neuroprotective properties, and their mechanisms of activity. The article presents a review of the identified therapeutic properties of phytomedicines that exhibit strong anti-Alzheimer’s disease (AD) activity.
Staff’s Knowledge and Self-Confidence in Difficult Communication: Evaluation of a Short Experiential-Based Training Program
Authors: Gonella Silvia , Brofferio Ludovica , Stella Luigi , Sciarrotta Daniele , Di Giulio Paola , Dimonte Valerio
Published: February 2025

Background: Most of the communication training programs developed over the past two decades were monodisciplinary, relied on traditional teaching methods, and targeted the hospital context. Objectives: The aim of this study is to assess the impact of a short, interdisciplinary, experiential-based communication program (Teach-to-Communicate program) targeted at nursing home (NH) personnel with regard to short-term, staff-related outcomes. Methods: This study is part of a larger quality improvement project based on a pre–post single-arm intervention design. We focused on a 6 h residential program involving 30 participants with different scopes of practice working in an NH. Traditional and experiential learning methods were used, including lectures, small group discussions, brainstorming sessions, videos on successful and failed communication, role play, and storytelling based on real cases. The primary outcome was the acquired knowledge of communication strategies and protocols (pre- and post-test quiz). Secondary outcomes were self-reported knowledge, preparedness, confidence, and satisfaction with training (5-point Likert questions). Results: A statistically significant improvement in acquired knowledge with a large size effect (0.7, p < 0.001) was observed. Self-reported preparedness and confidence ameliorated for all measured communication skills and topics, with the highest effect size registered for self-reported preparedness and confidence in engaging in difficult communication (both 0.7, p < 0.001). Participants were highly satisfied with the training, and particularly with the use of video cases (mean 4.6, SD 0.6) and the relevance to clinical practice (mean 4.7, SD 0.5). Conclusions: The Teach-to-Communicate program holds promise for improving knowledge and self-confidence regarding difficult communication among NH staff, and highlights the utility of video cases in communication skills training.
Simulated Practice Learning Experience in a Virtual Environment: An Innovative Pedagogical Approach to Practice Learning for Nursing Students
Authors: Faulds Sharon , Taylor Anne
Published: February 2025

Background/Objectives: The use of simulated learning as a teaching approach has been used and embedded in nursing theoretical curriculum for many years. There is a wealth of evidence to support the positive impact simulated learning or simulated-based education can have on the student experience, developing skill competency and enhancing patient outcomes. However, the evidence on the use of simulation as a replacement for clinical practice learning in undergraduate nursing education is limited. In response to the challenges posed by the COVID-19 pandemic, the authors introduced virtual simulated practice learning experiences (SPLE) for a cohort of year one pre-registration adult and mental health nursing students. The SPLE project aimed to assess the effectiveness of simulated practice learning as a viable alternative to traditional clinical practice learning and to explore student satisfaction with the new practice learning experience approach. Methods: All year one student nurses attending the four simulated practice learning experience (SPLE) weeks were invited to participate and complete a generated questionnaire within the virtual practice environment on their final day of each SPLE week. The questionnaire employed a mix of both quantitative and qualitative questions across key areas to evaluate the effectiveness of the SPLE and explore student satisfaction with their overall practice learning experience. Results: A total of 216 students participated in the simulated practice learning weeks across the spring semester in 2023 with a response rate of 98–100% across all four SPLE weeks. Students reported an overall satisfaction score of 88%, highlighting their preparedness and positive feedback on the organisation, delivery, and content of the SPLE. Qualitative analysis revealed key themes, including the development of transferable skills and personal growth, the value of peer learning, the benefits of a virtual environment, and appreciation of service user and healthcare professional input. Students reported significant personal growth, improved communication skills, and a deeper understanding of holistic care through interactive and collaborative learning experiences. Conclusions: This evaluation underscores the innovative potential of simulated practice learning to enhance nursing practice education, emphasising the importance of integrating emerging technologies and diverse pedagogical approaches. The findings suggest that SPLEs can effectively prepare nursing students for the complexities of clinical practice while addressing the evolving demands of healthcare. Future research should focus on longitudinal studies to assess the sustained impact of simulated learning on clinical experiences and professional development.
Using an Interpretive Phenomenological Approach to Understand the Menstrual Experience of Young Adults
Authors: Graeve Catherine , Stephenson Vera , Gao Grace
Published: February 2025

Background/Objectives: an estimated 1.8 billion people worldwide menstruate, and many face difficulties managing. Young adults often encounter stigma, exclusion, and a lack of resources to manage menstruation comfortably. A review of studies on college students revealed that menstrual symptoms and stigma lead to absenteeism, poorer performance at work and school, and decreased quality of life. This study examines the multifaceted perceptions of a diverse group of young adults aged 18–25 to gain a deeper understanding of the menstrual experience, including cultural stigma, to advocate for personalized care and policy change. Methods: we used snowball sampling and employed a qualitative research methodology integrating a Qualtrics survey (n = 620) and focus groups (n = 50). We used an interpretive phenomenological approach to thematically code participants’ lived experiences by assigning codes to text segments and grouping them by broader themes using the Nvivo 14.23.3 software to understand the underlying meaning and significance of the data. Results: a diverse group of young adults completed the study. Key themes include difficulties attending work/school/social activities related to the physical and mental health challenges associated with menstruation, a cultural stigma, and a lack of access to healthcare and care products during menstruation. Quality of life could be improved with decreased stigma and improved self-care strategies. Limitations include the small sample size and the qualitative study design. Conclusions: this study highlights the need for a holistic approach to supporting menstruation. Recommendations include increasing access to menstrual healthcare, an understanding of cultural differences, and consideration of policy accommodations such as paid time off by workplaces and educational institutions related to menstruation.
Mirtrons in Human Cancers
Authors: Chen Yi-Ling , Pascuzzi Nicholas , Ruiz Alejandro , Chen Kuan-Hui Ethan
Published: February 2025

Mirtrons represent a new subclass of microRNAs (miRNAs) that are processed through non-canonical biogenesis pathways. Unlike canonical miRNAs, which require Drosha-mediated cleavage, mirtrons are generated via the splicing of short intronic sequences, bypassing Drosha entirely. While mirtrons are found across a variety of organisms, their conservation between species is relatively low. This evolutionary divergence has resulted in mirtrons acquiring species-specific regulatory functions. In humans, mirtrons remain an understudied group of regulatory RNAs. However, emerging evidence highlights their critical roles in cancer biology. These small RNAs influence a range of oncogenic processes, including tumor initiation, progression, metastasis, and resistance to therapy. By directly regulating the expression of oncogenes and tumor suppressor genes, mirtrons serve as key molecular mediators within cellular signaling pathways. What sets mirtrons apart from canonical miRNAs is their unique mode of biogenesis and structural attributes, which reveal alternative regulatory mechanisms that could be exploited in cancer biology. Recent advances in understanding their functions suggest that mirtrons hold significant potential as biomarkers for cancer diagnosis and prognosis. Additionally, their role as modulators of cancer pathways positions them as promising therapeutic targets in precision oncology. This review delves into the growing body of research on mirtrons, focusing on their biogenesis, biological roles, and implications in cancer. By emphasizing their distinct features and clinical relevance, it aims to provide a comprehensive perspective on the potential applications of mirtrons in advancing cancer diagnostics and therapeutics.
Fighting Strategies Against Chagas’ Disease: A Review
Authors: Hernández-Flores Andrea , Elías-Díaz Debora , Cubillo-Cervantes Bernadeth , Ibarra-Cerdeña Carlos N. , Morán David , Arnal Audrey , Chaves Andrea
Published: February 2025

Chagas disease, caused by Trypanosoma cruzi, remains a significant public health challenge, particularly in Latin America, where it is one of the most neglected diseases and is primarily transmitted by triatomine insects. The disease exhibits complexity due to its diverse transmission routes, including vectorial and non-vectorial mechanisms such as blood transfusions and congenital transmission. Effective monitoring and control strategies are critical to mitigating its impact. This review focuses on current monitoring and control efforts, emphasizing the importance of enhanced surveillance systems, improved risk assessments, and integrated vector control programs. Surveillance plays a pivotal role in early detection and timely intervention, particularly in endemic regions, while vector control remains central to reducing transmission. Moreover, the development of novel diagnostic tools, treatments, and vaccines is a crucial step in advancing control efforts. This review also highlights the involvement of local governments, international organizations, and civil society in executing these strategies, stressing the need for sustained political commitment to ensure the success of public health programs. By addressing key challenges in monitoring, control, and prevention, this review aims to provide insights and recommendations to further global efforts in reducing the burden of Chagas disease.
A Simple Method for Measuring Agar Gel Strength
Authors: Shauli Lilach , Salomon Eitan
Published: February 2025

Seaweeds are the only source for phycocolloids. Commercial applications of phycocolloids depend on their chemical/physical properties, including their gel forming ability. Thus, gel strength values are important for seaweed growers and scientists. Gel strength measurements include the use of texture analyzers or rheometers, which are not always available for seaweed growers and scientists. Here, we describe a home-made apparatus for assessing gel strength through the weight of a water column required for breaking a gel surface. The system worked well at gel concentrations between 0.5 and 1.5%, giving values of 82–535 g cm−2 and 163–754 g cm−2 for agar and agarose gels, respectively. The lowest variations were obtained for gel sample volumes between 25 and 30 mL. The system was manually operated but no significant variations were observed between measurements conducted by the five different users. The readings were independent of the water column fill rate. The variations in gel strength values were similar to reports using other gel strength measuring devices. We propose the use of our apparatus as a flexible, affordable tool for the assessment of gel quality, which is suitable for research groups or seaweed farmers without access to expensive equipment, and with a need to quickly assess their seaweed of interest at a relevant time scale for cultivation or harvest.
Bessel Beams in Gyroanisotropic Crystals with Optical Activity
Authors: Egorov Yuriy , Sokolenko Bogdan , Aliev Aziz , Dzhemalyadinov Ruslan , Umerov Ervin , Rubass Alexander
Published: February 2025

Using a Bessel beam as an example, it is shown that such a beam with the initial circular polarization, propagating through an optically active medium devoid of linear birefringence, generates an optical vortex in the orthogonally polarized component. It is shown that a medium with genuine optical activity allows the mutual conversion of beams with radial and azimuthal polarization distributions. It is also shown that a considerably weak perturbation of a medium with genuine optical activity by linear birefringence leads to the destruction of an isotropic point. In the vicinity of this point, the optical activity is suppressed by linear birefringence, so that a medium with a combined type of anisotropy responds as a medium with genuine linear birefringence. The structure of the fields of the eigenmodes of Bessel beams in a birefringent uniaxial crystal with optical activity is similar to the structure of the modes of a medium with genuine optical activity. The findings of the current study are believed to expand the understanding of physical processes in the developing field of polarization and the phase profilometry of materials.
Soil Amendments and Slow-Release Urea Improved Growth, Physiological Characteristics, and Yield of Salt-Tolerant Rice Under Salt Stress Conditions
Authors: Li Rongyi , Guo Xiayu , Qi Yucheng , Wang Yuyuan , Wang Jianbo , Zhang Pengfei , Cheng Shenghai , He Wenli , Zhao Tingcheng , Li Yusheng , Li Lin , Ji Junchao , He Aibin , Ai Zhiyong
Published: February 2025

The present study aimed to investigate the effects of different soil amendments coupled with nitrogen fertilizer on the morpho-physiological characteristics and yield of salt-tolerant rice under saline conditions. The soil amendments, i.e., S1: zeolite amendment, S2: coconut coir amendment, S3: humic acid amendment, and S0: no amendment, and fertilizer treatments, i.e., N1: urea, N2: slow-release urea, and N0: no N fertilizer, were kept in main plots and sub-plots, respectively, in a split-plot design. The salt-tolerant variety ‘Shuangliangyou 138’ was exposed to 0.3% salt irrigation water. The results showed that during the entire growth period, compared to S0, the S1 and S3 treatments increased the SPAD values by an average of 6.3%and 5.5%, respectively, the leaf area index by an average of 24.5% and 19.8%, the canopy interception rate by an average of 11.5% and 4.1%, and the aboveground biomass by an average of 36.8% and 13.9%, respectively. Moreover, under S1 and S3 conditions, the tiller number per square meter, leaf water potential, leaf water content, and chlorophyll contents were also improved under the slow-release urea than urea. Moreover, slow-release urea promoted root vitality and nutrient absorption as well as enhanced the activity of antioxidant and nitrogen metabolism enzymes than urea under the S1 and S3 conditions. In sum, the rational application of soil amendments and slow-release urea could improve the rice productivity on saline-alkali land.
Synergistic Effects of Zinc Oxide Nanoparticles and Moringa Leaf Extracts on Drought Tolerance and Productivity of Cucurbita pepo L. Under Saline Conditions
Authors: Abdelkhalik Abdelsattar , Gyushi Mohammed A. H. , Howladar Saad M. , Kutby Abeer M. , Asiri Nouf A. , Baeshen Areej A. , Nahari Aziza M. , Alsamadany Hameed , Semida Wael M.
Published: February 2025

This study investigated the combined effects of zinc oxide nanoparticles (Nano-Zn) and moringa leaf extract (MLE) on squash plants grown under water stress conditions in saline soil during 2021–2022. The research compared full irrigation (100% ETc) with water deficit conditions (60% ETc). While water deficit negatively impacted plant growth, yield, and various physiological parameters, the sequential application of Nano-Zn (at 50 or 100 mg L−1) with MLE (3%) significantly mitigated these adverse effects. The combined treatment proved more effective than individual applications, enhancing growth parameters, photosynthetic efficiency, and antioxidant systems. The treatment particularly improved stress tolerance by increasing protective compounds like soluble sugars and amino acids while reducing harmful H2O2 levels. The study concluded that sequential application of 100 mg L−1 Nano-Zn with MLE was optimal for enhancing squash performance under drought stress, with 50 mg L−1 Nano-Zn plus MLE as the second-best option.
Changes in the Suitable Habitat of the Smoke Tree (Cotinus coggygria Scop.), a Species with an East Asian–Tethyan Disjunction
Authors: Zhang Zichen , Yan Xin , Guo Chang , Dong Wenpan , Zhao Liangcheng , Liu Dan
Published: February 2025

The smoke tree (Cotinus coggygria Scop.) is a woody species mainly distributed in the Mediterranean region and East Asia, known for its high ecological and ornamental value. Investigation of changes in suitable habitats under different conditions can provide valuable insights with implications for predicting the distribution of C. coggygria. In this study, we employed a MaxEnt model to simulate the current, historical, and future suitable habitat of C. coggygria using distribution records and environmental variables. The results indicated that climatic variables had a much stronger impact on the suitable habitat of this species compared with soil and topographic variables, and bio11 (mean temperature of the coldest quarter) and bio12 (annual precipitation) played particularly important roles in determining the suitable habitat. The core distribution of C. coggygria exhibited an East Asian–Tethyan disjunction. During the glacial period (Last Glacial Maximum), C. coggygria in Europe was concentrated in the glacial refugia in southern Europe; its range was substantially smaller during the glacial period than during interglacial periods (mid-Holocene). In contrast, C. coggygria in East Asia survived in regions similar to those of the interglacial period. Future climate change led to a gradual northward expansion of suitable habitats for C. coggygria, and the area of suitable habitat was substantially larger in Europe than in East Asia. There were significant differences among the four climate scenarios in Europe, with minimal variation in East Asia. Our findings provide valuable insights into the contrasting effects of climate change on European and East Asian populations of C. coggygria, which enhances our understanding of Eurasian species with discontinuous distributions.
Plant Diversity and Sustainable Landscape Management: The Case of Misiliscemi, a New Municipality in Sicily
Authors: Aleo Michele , Bazan Giuseppe
Published: February 2025

Floristic and biodiversity knowledge play a crucial role in ecosystem conservation and sustainable land management, particularly in urban-rural contexts that can serve as biodiversity reservoirs, hosting species of high biogeographic value. Focusing on the new municipality of Misiliscemi, established in 2021 in Sicily and now facing the challenge of developing new management strategies, this study provides fundamental knowledge on the plant biodiversity of the area and explores how the integration of floristic and environmental data can guide territorial planning strategies aimed at preserving natural capital and ecosystem services. The research, based on field surveys conducted over many years, taxonomic identification of species, analysis of biological forms and chorological data, evaluation of ecological indicators, and GIS-based habitat mapping according to the EUNIS classification, has made it possible to obtain a comprehensive dataset. The results of this work led to the identification of 623 taxa, recording new findings for the Sicilian flora, including both native and alien species, which represent primary biodiversity data crucial for plant resource management. In addition, 42 habitat types were mapped, highlighting that approximately 80% of the territory is occupied by vegetated man-made habitats. Despite anthropogenic pressures and landscape modifications, Misiliscemi retains significant plant biodiversity, including habitats and species of conservation interest, that represent a vital resource for natural capital and ecosystem services. This knowledge base, in addition to constituting the scientific foundation upon which this young municipality can develop an urban planning strategy aimed at achieving sustainable local development, also represents a methodological approach that highlights how basic knowledge of urban biodiversity should be considered a crucial aspect of sustainable urban planning worldwide.
Transcriptome and Metabolome Profiling Provide Insights into Flavonoid Biosynthesis and the Mechanism of Color Formation in Zanthoxylum bungeanum Maxim.
Authors: Yang Lin , Tian Lu , Shi Jingwei , Wei Anzhi
Published: February 2025

The color of Zanthoxylum bungeanum Maxim. (Z. bungeanum) is a key quality indicator and a factor limiting the development of its industry. However, the underlying mechanisms governing color formation remain largely unexplored. In this study, an integrative analysis of transcriptome and metabolome profiles was conducted across four developmental stages to elucidate the color formation mechanism in Z. bungeanum. A total of 137 flavonoids were identified as the fruits ripened, with high levels of differentially accumulated metabolites (DAMs), including tricetin and (-)-epigallocatechin, which were strongly associated with color formation. This suggests their significant contribution to the pigmentation process. Nine differentially expressed genes (DEGs) were identified as candidate genes involved in color development. Additionally, 15 transcription factors (TFs) (12 MYB and 3 bHLH) exhibited expression patterns similar to those of structural genes in the flavonoid biosynthetic pathway, indicating their role in regulating flavonoid synthesis. The bioinformatics analysis of three key flavonoid synthesis genes—ZbCHI, ZbFLS, and ZbANR—revealed that all three proteins exhibit hydrophobic structures without transmembrane domains. Among them, ZbANR possesses signal peptide regions, whereas ZbCHI and ZbFLS do not. Subcellular localization predictions suggest that ZbCHI is most likely localized in the chloroplast, ZbFLS in the cytoplasm, and ZbANR in the membrane. Functional analyses revealed that their transient expression in Nicotiana benthamiana (N. benthamiana) increased the flavonoid content, with ZbANR overexpression producing a distinct white phenotype in the plants. This study enriches transcriptomic data and provides a comprehensive understanding of flavonoid metabolism and the molecular basis of color formation in Z. bungeanum, offering a valuable theoretical foundation for future breeding programs.
Characterization of a Diverse Okra (Abelmoschus esculentus L. Moench) Germplasm Collection Based on Fruit Quality Traits
Authors: Yildiz Mehtap , Sirke Sibel Turan , Koçak Metin , Mancak İbrahim , Özkaya Aslıhan Agar , Abak Kazım , Özkaya Okan , Cavagnaro Pablo Federico
Published: February 2025

Okra is an important dietary component of many Asian countries, providing high levels of dietary fiber, phytonutrients (e.g., antioxidant vitamins and pigments), and essential minerals. Evaluation of okra germplasm collections can improve the curation of genebanks and help identify superior materials for breeding. In this study, 66 okra accessions from diverse geographical origins were characterized based on fruit quality traits, including fruit fresh (FFW) and dry weights (FDW), dry matter (DM), diameter, length, and diameter of the fruit peduncle; concentration of vitamin C, chlorophyll a and b, and total chlorophyll; and color-chroma values. Significant (p < 0.05) and substantial variation was found among the accessions for all traits. Mean FFW and FDW varied nearly three-fold, with ranges of 3.76–9.99 g and 0.43–1.34 g, respectively, with a range in DM content of 10.5–19.4%. Vitamin C and total chlorophyll content varied 6.4- and 8.3-fold, with ranges of 12.8–82.8 and 1.07–8.91 mg/100 g fw, respectively, with substantial variation also observed in chlorophyll composition. Significant positive correlations were found between vitamin C and total and subtypes of chlorophyll levels (r = 0.29–0.32), whereas the strongest correlations were between FFW and FDW (r = 0.88) and between total chlorophyll and chlorophyll subtypes a and b (r = 0.90–0.95). Additionally, a dendrogram constructed based on these phenotypic data grouped the accessions in general agreement with their geographical origins and fruit traits. Overall, our results revealed broad phenotypic diversity in the evaluated germplasm, which is exploitable in okra breeding programs aimed at increasing fruit quality and nutraceutical value.
Neural Network Modeling of CuO/Au Hybrid Nanofluid Thermal Performance with Slip Effects for Advanced Process Applications
Authors: Kotike Jyothi , Beedalannagari Omprakash , Rekapalli Leelavathi , Usman Muhammad , Kadavakollu Kalyani Radha
Published: February 2025

This study explores transient magnetohydrodynamic (MHD) heat and mass transfer in the flow of a hybrid nanofluid over a stretching surface, considering both steady and unsteady scenarios. The investigation incorporates chemical reactions, slip boundary conditions, and the effects of thermal radiation. The hybrid nanofluid, composed of copper oxide (CuO) and gold (Au) nanoparticles in a water-based fluid, demonstrates enhanced thermal performance compared with base fluids. Key findings reveal that higher nanoparticle concentrations significantly improve heat transfer, highlighting the potential of hybrid nanofluids in advanced thermal management applications. Additionally, machine learning models effectively predict heat transfer characteristics with high accuracy (R2 = 0.99), showcasing their effectiveness in complementing traditional numerical methods. These findings contribute to the understanding of hybrid nanofluids in complex thermal systems and highlight the utility of emerging computational tools for thermal analysis.
Systems Biology of Recombinant 2G12 and 353/11 mAb Production in CHO-K1 Cell Lines at Phosphoproteome Level
Authors: Sulaj Eldi , Sandell Felix L. , Schwaigerlehner Linda , Marzban Gorji , Dohm Juliane C. , Kunert Renate
Published: February 2025

Background: Chinese hamster ovary (CHO) cells are extensively used in the pharmaceutical industry for producing complex proteins, primarily because of their ability to perform human-like post-translational modifications. However, the efficiency of high-quality protein production can vary significantly for monoclonal antibody-producing cell lines, within the CHO host cell lines or by extrinsic factors. Methods: To investigate the complex cellular mechanisms underlying this variability, a phosphoproteomics analysis was performed using label-free quantitative liquid chromatography after a phosphopeptide enrichment of recombinant CHO cells producing two different antibodies and a tunicamycin treatment experiment. Using MaxQuant and Perseus for data analysis, we identified 2109 proteins and quantified 4059 phosphosites. Results: Significant phosphorylation dynamics were observed in nuclear proteins of cells producing the difficult-to-produce 2G12 mAb. It suggests that the expression of 2G12 regulates nuclear pathways based on increases and decreases in phosphorylation abundance. Furthermore, a substantial number of changes in the phosphorylation pattern related to tunicamycin treatment have been detected. TM treatment affects, among other phosphoproteins, the eukaryotic elongation factor 2 kinase (Eef2k). Conclusions: The alterations in the phosphorylation landscape of key proteins involved in cellular processes highlight the mechanisms behind stress-induced cellular responses.
Meta-Research in Biomedical Investigation: Gaps and Opportunities Based on Meta-Research Publications and Global Indicators in Health, Science, and Human Development
Authors: Lozada-Martinez Ivan David , Hernandez-Paz David A. , Fiorillo-Moreno Ornella , Picón-Jaimes Yelson Alejandro , Bermúdez Valmore
Published: February 2025

Meta-research in biomedical science is crucial for ensuring rigour, relevance, and transparency in an era marked by the exponential growth of scientific publications. This study examines global and historical trends in meta-research activities within biomedicine and investigates their relationship with health, science, and human development indicators. A systematic analysis of 9633 publications from Scopus, Web of Science, and PubMed was conducted, focusing on publication volume, citation impact, and geographic distribution. Regression analyses reveal a significant positive association between meta-research activity and the Human Development Index (HDI), suggesting that meta-research contributes to societal advancement by enhancing evidence-based decision-making in health. However, no association was found between meta-research output and research and development (R&D) expenditure, reflecting the minimal resource requirements of secondary data-driven studies compared to primary or experimental research. Meta-research activity correlates positively with clinical trial completion, indicating its role in refining study designs and addressing evidence gaps. These findings highlight the importance of expanding meta-research in underrepresented regions to promote equity in scientific advancement and improve the reliability of biomedical knowledge. This result underscores the need for targeted support for meta-research, particularly in low- and middle-income countries with limited scientific infrastructure and resources for new knowledge generation.
Resilience in Pontifical Doctrines: From Pope Benedict XVI to Pope Francis
Authors: Figus-Illinyi Rita
Published: February 2025

This study explores the concept of resilience within the teachings of Popes Benedict XVI and Francis, comparing these with established psychological resilience theories by Ungar and Holling. Through a comprehensive analysis of documents sourced from the Vatican’s official website, resilience is examined across individual, community, and global dimensions. Individual resilience emphasizes spiritual resources such as faith and hope, which Pope Benedict XVI and Pope Francis underscore as essential for overcoming personal and societal crises. Community resilience is highlighted in the context of solidarity, cooperation, and cultural identity, as demonstrated in responses to natural disasters and sociopolitical challenges. At a global level, Pope Francis advocates ecological sustainability and systemic justice, tying resilience to shared responsibilities and global solidarity. Methods include textual frequency analysis and semantic mapping of resilience-related terms within papal documents, complemented by a comparative analysis with psychological resilience frameworks. Findings reveal unique contributions of papal teachings, such as the integration of spiritual, moral, and ecological dimensions, which expand traditional resilience concepts. This theological lens adds normative and prescriptive elements, offering transformative perspectives for resilience studies, emphasizing faith, values, and sustainability as pivotal components for enduring and thriving amidst adversity. Limitations of data mining methods suggest potential for further interdisciplinary research.
The Disenchantment of Hell and the Emergence of Self-Conscious Individuality: Examining Su Shi’s Philosophy of Disposition
Authors: Xu Shuang , Ni Yicai
Published: February 2025

In pre-Song Chinese thought, the afterlife, or the subterranean realm was a sacred space distinctly separate from the world of the living, an extension of the political–religious–cultural order of the Chinese empire. Even after the introduction of Buddhism to China, although the Buddhist concept of hell applied karmic retribution to the present life in an attempt to provide ethical norms for real life, the sacredness of the afterlife remained intact. Song Dynasty Chinese thought underwent a profound “modernization” transformation. Su Shi, with the concept of qing 情 [disposition] at its core, disenchanted the sacred afterlife, shaping a new, self-conscious individuality in the interplay between the living and the dead, the finite and the infinite, the mortal and the immortal. This new individuality, with its spiritual spontaneity and freedom, integrated the afterlife with the present world, internalizing infinity and immortality into a utopian spiritual homeland. This free individuality, entirely different from Song-Ming Neo-Confucianism, marking a hidden potential in the development of Chinese intellectual history that has yet to be fully revealed.
Reclaiming the Christ-Centered Pulpit: A Theological Exploration of Redemptive Preaching
Authors: Greene Bob C.
Published: February 2025

This article embarks on a profound theological journey through the sacred Scriptures, illuminating Jesus Christ as the central figure of the entire biblical narrative. It posits that the ultimate purpose of preaching, emphasized in this discourse, is the radiant glory of God in Christ, reflected in the joyful submission of His creation. Through meticulous exploration, this article reveals the crucial need to restore the authority of the Gospel, highlighting the pivotal role of Christ-centered preaching in deepening our understanding of God’s redemptive design. At the outset, this article conveys the significance of approaching Scripture with an unwavering commitment to discover Christ within its pages. The preacher’s quest to find Jesus in every text becomes a theological mandate, especially when engaging with the Old Testament, where Christ’s presence may seem less evident. The article asserts that sermon preparation should consistently center around some aspect of the person and work of Christ, ultimately leading to the presentation of the Gospel—a proclamation of Jesus Christ’s incarnation, life, death, burial, resurrection, and ascension.