bluuebunny/crossref_metadata_2025 huggingface dataset:
Dried blood spots for Streptococcus pneumoniae and Haemophilus influenzae detection and serotyping among children < 5 years old in rural Mozambique
Authors: Pimenta Fabiana C. , Moiane Benild , Lessa Fernanda C. , Venero Anne-Kathryn L. , Moura Iaci , Larson Shanda , Massora Sergio , Chaúque Alberto , Tembe Nelson , Mucavele Helio , Verani Jennifer R. , Whitney Cynthia G. , Sigaúque Betuel , Carvalho Maria G. S.
Published: July 2020

Background

Dried blood spots (DBS) have been proposed as potentially tool for detecting invasive bacterial diseases.

Methods

We evaluated the use of DBS for S. pneumoniae and H. influenzae detection among children in Mozambique. Blood for DBS and nasopharyngeal (NP) swabs were collected from children with pneumonia and healthy aged < 5 years. Bacterial detection and serotyping were performed by quantitative PCR (qPCR) (NP and DBS; lytA gene for pneumococcus and hpd for H. influenzae) and culture (NP). Combined detection rates were compared between children with pneumonia and healthy.

Results

Of 325 children enrolled, 205 had pneumonia and 120 were healthy. Pneumococci were detected in DBS from 20.5 and 64.2% of children with pneumonia and healthy, respectively; NP specimens were positive for pneumococcus in 80.0 and 80.8%, respectively. H. influenzae was detected in DBS from 22.9% of children with pneumonia and 59.2% of healthy; 81.4 and 81.5% of NP specimens were positive for H. influenzae, respectively.

Conclusion

DBS detected pneumococcal and H. influenzae DNA in children with pneumonia and healthy. Healthy children were often DBS positive for both bacteria, suggesting that qPCR of DBS specimens does not differentiate disease from colonization and is therefore not a useful diagnostic tool for children.
Association between suicide risk severity and sarcopenia in non-elderly Chinese inpatients with major depressive disorder
Authors: Fan Xin-Xin , Yuan Jing , Wei Yu-Jun , Zhou Fang , Xu Li , Zhang Yan , Meng Jun-Yu , Jin Xiao-Long , Yang Jian-Zhong
Published: July 2020

Background

Sarcopenia is a skeletal muscle disorder. Recent studies have shown an association between muscle health and suicide. However, there have been no previous studies on the relationship between suicide risk severity and sarcopenia in major depressive disorder (MDD). This study aimed to explore the association between suicide risk severity and sarcopenia in non-elderly Chinese inpatients with MDD.

Methods

The first-episode drug-naïve MDD inpatients aged 20–59 years with the 24-item Hamilton Rating Scale for Depression (HAMD-24) scores of >20 were included, who were then classified into low, intermediate, high and very high suicide risk groups according to the Nurses’ Global Assessment of Suicide Risk (NGASR). The HAMD-24, the Hamilton Rating Scale for Anxiety (HAMA) and the SARC-F questionnaire were used to assess depression severity, anxiety severity and sarcopenia, respectively. The plasma levels of cortisol and adrenocorticotropic hormone (ACTH) were measured.

Results

A total of 192 MDD inpatients (122 females, 70 males; aged 39.3 ± 11.7 years) were included, with 12.5% meeting criteria for sarcopenia. There were significant differences in gender, HAMD score and prevalence of sarcopenia among the suicide risk groups. Adjusted ordinal regression analysis showed that sarcopenia was significantly associated with more severe suicide risk (OR = 2.39, 95%CI 1.02–5.58,p = 0.044) independent of depression severity.

Conclusions

This study revealed that sarcopenia was significantly associated with higher suicide risk in non-elderly Chinese MDD inpatients after adjustment for depression severity. Intervention of sarcopenia might be effective in reducing the risk of suicide in non-elderly MDD patients.
Food supplements to reduce stunting in Pakistan: a process evaluation of community dynamics shaping uptake
Authors: Zaidi Shehla , Das Jai K. , Khan Gul Nawaz , Najmi Rabia , Shah Mashal Murad , Soofi Sajid B.
Published: July 2020

Background

There is an increasing interest in use of food supplements to prevent childhood stunting, however the evidence on the process indicators is scarce. We in this study explore the barriers to the effective implementation of food supplementation programs and the possible mitigation strategies which can guide the design of future programs.

Methods

We undertook a process evaluation of a stunting prevention food supplementation pilot program in rural Pakistan that distributed Wheat Soy Blend (WSB) to pregnant & lactating women, and Lipid-based Nutrient Supplement (LNS) and micronutrient powder (MNP) to < 5 years children. We used a mixed methods approach through a quantitative survey of 800 households and conducted 18 focused group discussion (FGDs) (with male and female caregivers), 4 FGDs (with Community Health Workers (CHWs)) and 22 key informant interviews (with district stakeholders) to evaluate the community side factors affecting uptake through five parameters: value, acceptability, receipt of supplement, usage and correct dosage.

Results

The findings show that proportionately few beneficiaries consumed the full dose of supplements, despite reasonable knowledge amongst caregivers. Sharing of supplements with other household member was common, and the full monthly stock was usually not received. Qualitative findings suggest that caregivers did not associate food supplements with stunting prevention. WSB was well accepted as an extra ration, LNS was popular due its chocolaty taste and texture, whereas MNP sprinkles were perceived to be of little value. The cultural food practices led to common sharing, whereas interaction with CHWs was minimal for nutrition counselling. Qualitative findings also indicate CHWs related programmatic constraints of low motivation, multi-tasking, inadequate counselling skills and weak supervision.

Conclusion

We conclude that the community acceptability of food supplements does not translate into optimal consumption. Hence a greater emphasis is needed on context specific demand creation and focusing on the supply side constraints with improved logistical planning, enhanced motivation and supervision of community workers with involvement of multiple stakeholders. While, similar studies are needed in varying contexts to help frame universal guidelines.

Trial registration

ClinicalTrials.gov Identifier: NCT02422953. Registered on April 22, 2015.
Wellness project implementation within Houston’s Faith and Diabetes initiative: a mixed methods study
Authors: Wells Rebecca , Breckenridge Ellen D. , Linder Stephen H.
Published: July 2020

Background

Faith-based health promotion has shown promise for supporting healthy lifestyles, but has limited evidence of reaching scale or sustainability. In one recent such effort, volunteers from a diverse range of faith organizations were trained as peer educators to implement diabetes self-management education (DSME) classes within their communities. The purpose of this study was to identify factors associated with provision of these classes within six months of peer-educator training.

Methods

This study used the Consolidated Framework for Implementation Research (CFIR) to identify patterns from interviews, observations, attendance records, and organizational background information. Two research team members thematically coded interview transcripts and observation memos to identify patterns distinguishing faith organizations that did, versus did not, conduct DSME classes within six months of peer-educator training. Bivariate statistics were also used to identify faith organizational characteristics associated with DSME class completion within this time frame.

Results

Volunteers from 24 faith organizations received peer-educator training. Of these, 15 led a DSME class within six months, graduating a total of 132 participants. Thematic analyses yielded two challenges experienced disproportionately by organizations unable to complete DSME within six months: [1] Their peer educators experienced DSME as complex, despite substantial planning efforts at simplification, and [2] the process of engaging peer educators and leadership within their organizations was often more difficult than anticipated, despite initial communication by Faith and Diabetes organizers intended to secure informed commitments by both groups. Many peer educators were overwhelmed by training content, the responsibility required to start and sustain DSME classes, and other time commitments. Other priorities competed for time in participants’ lives and on organizational calendars, and scheduling processes could be slow. In an apparent dynamic of “crowding out,” coordination was particularly difficult in larger organizations, which were less likely than smaller organizations to complete DSME classes despite their more substantial resources.

Conclusions

Initial commitment from faith organizations’ leadership and volunteers may not suffice to implement even relatively short and low cost health promotion programs. Faith organizations might benefit from realistic previews about just how challenging it is to make these programs a sufficiently high organizational and individual priority.
The risk of fracture and prevalence of osteoporosis is elevated in patients with idiopathic inflammatory myopathies: cross-sectional study from a single Hungarian center
Authors: Vincze Anett , Bodoki Levente , Szabó Katalin , Nagy-Vincze Melinda , Szalmás Orsolya , Varga József , Dankó Katalin , Gaál János , Griger Zoltán
Published: July 2020

Background

The prevalence of osteoporosis and risk of fractures is elevated in rheumatoid arthritis (RA), but we have limited information about the bone mineral density (BMD) and fracture risk in patients with inflammatory myopathies. We intended to ascertain and compare fracture risk, bone mineral density and the prevalence of vertebral fractures in patients with inflammatory myositis and rheumatoid arthritis and to assess the effect of prevalent fractures on the quality of life and functional capacity.

Methods

Fifty-two patients with myositis and 43 patients with rheumatoid arthritis were included in the study. Fracture Risk was determined using FRAX® Calculation Tool developed by the University of Sheffield. Dual energy X-ray absorptiometry and bidirectional thoracolumbar radiographs were performed to assess BMD and vertebral fractures. Quality of life was measured with Short Form-36 (SF-36) and physical function assessment was performed using Health Assessment Questionnaire (HAQ).

Results

We found a significantly elevated fracture risk in RA as compared to myositis patients if the risk assessment was performed without the inclusion of the BMD results. If BMD results and glucocorticoid dose adjustment were taken into account, the differences in fracture risk were no longer significant. The prevalence of osteoporosis was found to be significantly higher in the myositis group (7% vs. 13.5%, p: 0.045), but the fracture prevalence was similar in the two groups (75% vs. 68%). The fracture rates were independently associated with age in the myositis group, and with lower BMD results in the RA patients. The number of prevalent fractures was significantly correlated to poorer physical function in both groups, and poorer health status in the myositis group, but not in the RA group.

Conclusions

Our findings suggest that inflammatory myopathies carry significantly elevated risks for osteoporosis and fractures. These higher risks are comparable to ones detected with RA in studies and strongly affect the physical function and quality of life of patients. Therefore further efforts are required to make the fracture risk assessment reliable and to facilitate the use of early preventive treatments.
Structure and mechanical properties of high-weight-bearing and low-weight-bearing areas of hip cartilage at the micro- and nano-levels
Authors: Guo Jiang-Bo , Liang Ting , Che Yan-Jun , Yang Hui-Lin , Luo Zong-Ping
Published: July 2020

Background

Articular cartilage has a high-weight-bearing area and a low-weight-bearing area, the macroscopic elastic moduli of the two regions are different. Chondrocytes are affected by the applied force at the microscopic level. Currently, the modulus of the two areas at the micro and nano levels is unknown, and studies on the relationship between macro-, micro- and nano-scale elastic moduli are limited. Such information may be important for further understanding of cartilage mechanics. Moreover, the surface morphology, proteoglycan content, and micro and nano structure of the two areas, which influences the mechanical properties of cartilage should be discussed.

Methods

Safranin-O/Fast Green staining was used to evaluate the surface morphology and semi-quantify proteoglycan content of porcine femoral head cartilage between the two weight-bearing areas. The unconfined compression test was used to determine the macro elastic modulus. Atomic force microscope was used to measure the micro and nano compressive elastic modulus as well as the nano structure. Scanning electron microscope was employed to evaluate the micro structure.

Results

No significant differences in the fibrillation index were observed between two areas (P = 0.5512). The Safranin-O index of the high-weight-bearing area was significantly higher than that of the low-weight-bearing area (P = 0.0387). The compressive elastic modulus of the high-weight-bearing area at the macro and micro level was significantly higher than that of the low-weight-bearing area (P = 0.0411 for macro-scale, and P = 0.0001 for micro-scale), while no statistically significant differences were observed in the elastic modulus of collagen fibrils at the nano level (P = 0.8544). The density of the collagen fibers was significantly lower in the high-weight-bearing area (P = 0.0177). No significant differences were observed in the structure and diameter of the collagen fibers between the two areas (P = 0.7361).

Conclusions

A higher proteoglycan content correlated with a higher compressive elastic modulus of the high-weight-bearing area at the micro level than that of the low-weight-bearing area, which was consistent with the trend observed from the macroscopic compressive elastic modulus. The weight-bearing level was not associated with the elastic modulus of individual collagen fibers and the diameter at the nano level. The micro structure of cartilage may influence the macro- and micro-scale elastic modulus.
Small renal masses in Latin-American population: characteristics and prognostic factors for survival, recurrence and metastasis – a multi-institutional study from LARCG database
Authors: Mourão Thiago Camelo , Abreu Diego , Carvalhal Gustavo F. , Gueglio Guillermo , da Costa Walter H. , Calsavara Vinicius Fernando , Meza-Montoya Luis , Bengió Rubén G. , Scorticati Carlos , Castillejos-Molina Ricardo , Rodríguez-Covarrubias Francisco , Autran-Gómez Ana María , Campos-Salcedo José Gadu , Nolazco Alejandro , Ameri Carlos , Zampolli Hamilton , Langenhin Raúl , Muguruza Diego , Machado Marcos Tobias , Mingote Pablo , Clavijo Jorge , Nogueira Lucas , Clark Omar , Rovegno Agustín R. , Secin Fernando P. , Decia Ricardo , Guimarães Gustavo C. , Glina Sidney , Rodríguez-Faba Oscar , Palou Joan , Zequi Stenio C.
Published: July 2020

Background

To evaluate demographic, clinical and pathological characteristics of small renal masses (SRM) (≤ 4 cm) in a Latin-American population provided by LARCG (Latin-American Renal Cancer Group) and analyze predictors of survival, recurrence and metastasis.

Methods

A multi-institutional retrospective cohort study of 1523 patients submitted to surgical treatment for non-metastatic SRM from 1979 to 2016. Comparisons between radical (RN) or partial nephrectomy (PN) and young or elderly patients were performed. Kaplan-Meier curves and log-rank tests estimated 10-year overall survival. Predictors of local recurrence or metastasis were analyzed by a multivariable logistic regression model.

Results

PN and RN were performed in 897 (66%) and 461 (34%) patients. A proportional increase of PN cases from 48.5% (1979–2009) to 75% (after 2009) was evidenced. Stratifying by age, elderly patients (≥ 65 years) had better 10-year OS rates when submitted to PN (83.5%), than RN (54.5%), p = 0.044. This disparity was not evidenced in younger patients. On multivariable model, bilaterality, extracapsular extension and ASA (American Society of Anesthesiologists) classification ≥3 were predictors of local recurrence. We did not identify significant predictors for distant metastasis in our series.

Conclusions

PN is performed in Latin-America in a similar proportion to developed areas and it has been increasing in the last years. Even in elderly individuals, if good functional status, sufficiently fit to surgery, and favorable tumor characteristics, they should be encouraged to perform PN. Intending to an earlier diagnosis of recurrence or distant metastasis, SRM cases with unfavorable characteristics should have a more rigorous follow-up routine.
Drain fluid amylase as a predictor of postoperative salivary fistula in cases with benign parotid tumours
Authors: Lu Yusheng , Zhang Shijian , Peng Canbang , Yang Wenyi , Zhang Chenping , Ren Zhenhu
Published: July 2020

Background

Late diagnosis of a salivary fistula increases the risk of wound infection and scarring. The purpose of the present study was to identify a quantitative predictor of postoperative salivary fistula for cases treated with surgery.

Methods

Demographic, intraoperative and postoperative parameters for 57 cases that received surgery for benign parotid tumours were recorded from June 2017 to June 2018, of which 18 cases developed salivary fistulas. These data were analysed using univariate and binary logistic regression analyses as well as receiver operating curve analysis.

Results

Drain fluid amylase concentration was positively correlated with salivary fistula development (p <  0.001), with an odds ratio of 1.14 for a 1 KU/L increase in concentration and an optimal receiver operating curve cut-off value of 51,100 U/L for predicting salivary fistula development. Cases wherein the parotid–masseteric fascia remained intact were associated with a lower risk of salivary fistula development (p = 0.006).

Conclusion

Drain fluid amylase concentration may be a valuable predictor of postoperative salivary fistula in cases with benign parotid tumours.
Patients’ and clinicians’ expectations on integrative medicine Services for Diabetes: a focus group study
Authors: Chan Kam Wa , Lee Pak Wing , Leung Crystal Pui Sha , Chan Gary Chi Wang , Yiu Wai Han , Cheung Hoi Man , Li Bin , Lok Sarah Wing Yan , Li Hongyu , Xue Rui , Chan Loretta Yuk Yee , Leung Joseph Chi Kam , Lam Tai Pong , Lai Kar Neng , Tang Sydney Chi Wai
Published: July 2020

Background

Difference of perspective between patients and physicians over integrative medicine (IM) research and service provision remains unclear despite significant use worldwide. We observed an exceptionally low utilisation of IM and potential underreporting in diabetes. We aimed to explore the barriers and recommendations regarding service delivery and research of IM service among diabetes patients and physicians.

Methods

A 10-group, 50-participant semi-structured focus group interview series was conducted. Twenty-one patients with diverse severity of disease, comorbidities and education levels; and 29 physicians (14 conventional medicine (ConM) and 15 Chinese medicine (CM)) with diverse clinical experience, academic background and affiliation were purposively sampled from private and public clinics. Their perspectives were qualitatively analysed by constant comparative method.

Results

Seven subthemes regarding barriers towards IM service were identified including finance, service access, advice from medical professionals, uncertainty of service quality, uncertainty of CM effect, difficulty in understanding CM epistemology and access to medical records. Patients underreported the use of CM due to the concern over neutrality of medical advice among physicians. Inconvenience of service access, frequent follow-up, use of decoction and long-term financial burden were identified as key obstacles among patients. Regarding research design, ConM physicians emphasised standardisation and reproducibility while CM physicians emphasised personalisation. Some CM-related outcome measurements were suggested as non-communicable. Both physicians acknowledged the discordance in epistemology should be addressed by pragmatic approach.

Conclusion

Key obstacles of CAM clinical utilisation are different between patients. Further assessment on IM should be pragmatic to balance between standardisation, reproducibility and real-world practice. Evidence-based IM programs and research should merge with existing infrastructure.
A comparative study on the prediction of the BP artificial neural network model and the ARIMA model in the incidence of AIDS
Authors: Li Zeming , Li Yanning
Published: July 2020

Background

As a kind of widely distributed disease in China, acquired immune deficiency syndrome (AIDS) has been quickly growing each year, become a serious problem and caused serious damage to the life and health of people and the social events of China and the world because of its high fatality rate. It has been much concerned by all aspects of society. Therefore, developing early warning technology and finding the trend of early development are of quite significance to prevent and control human immunodeficiency virus (HIV)/AIDS. This study aimed to explore a suitable model for the morbidity of AIDS in China and establish a professional and feasible disease prediction model for the prevention and control works of AIDS.

Methods

At present, the traditional linear model is still utilized by most scholars to predict the incidence of HIV/AIDS. In addition, some scholars may attempt to use the nonlinear prediction model. Both prediction models showed good fitting and prediction effects. In China, the incidence of AIDS presents linear and nonlinear characteristics. In this research, the nonlinear back propagation artificial neural network (BP-ANN) model and the typical auto-regressive integrated moving average (ARIMA) linear model were applied to predict the incidence of HIV/AIDS and compare their fitting effects.

Results

Both models were capable of predicting the expected cases of AIDS. It was seen that ARIMA and BP-ANN models could be used to forecast the monthly incidence of HIV/AIDS, but the fitting and forecasting effects of the nonlinear BP neural network model were better than those of the traditional linear ARIMA model.

Conclusions

In summary, it was further concluded that the BP-ANN model was a suitable way to monitor and predict the change trend and morbidity of AIDS in China.
Evaluation of the association between presenteeism and perceived availability of social support among hospital doctors in Zhejiang, China
Authors: Xi Xiaoyu , Lu Qianni , Lu Mengqing , Xu Ailin , Hu Hao , Ung Carolina Oi Lam
Published: July 2020

Background

This study investigated the association between presenteeism and the perceived availability of social support among hospital doctors in China.

Methods

A questionnaire was administered by doctors randomly selected from 13 hospital in Hangzhou China using stratified sampling. Logit model was used for data analysis.

Results

The overall response rate was 88.16%. Among hospital doctors, for each unit increase of the perceived availability of social support, the prevalence of presenteeism was decreased by 8.3% (OR = 0.91, P = 0.000). In particular, if the doctors perceived availability of appraisal support, belonging support and tangible support as sufficient, the act of presenteeism was reduced by 20.2% (OR = 0.806, P = 0.000) 20.4% (OR = 0.803, P = 0.000) and 21.0% (OR = 0.799, P = 0.000) respectively with statistical differences.

Conclusion

In China, appraisal support, belonging support and tangible support, compared to other social support, had a stronger negative correlation with presenteeism among hospital doctors. The benefits of social support in alleviating doctors’ presenteeism warrant further investigation.
Pharmacists' viewpoint towards their professional role in healthcare system: a survey of hospital settings of Pakistan
Authors: Khan Nabeel , McGarry Ken , Naqvi Atta Abbas , Iqbal Muhammad Shahid , Haider Zaki
Published: July 2020

Background

Pharmacy service is an essential part of a healthcare system. The profession of pharmacy is well recognized and is practiced to its full potential in developed countries however, it is underutilized in developing countries such as Pakistan. The recognition of pharmacist’s role as healthcare professional is limited. This study aimed to document pharmacists’ attitude towards their role in Pakistan’s healthcare system, their experience with doctors and their perceptions towards involvement in medicines management.

Methods

A 4-month cross-sectional survey (Jan – Apr 18) was conducted targeting pharmacists practising in 26 tertiary care hospitals across Pakistan using a developed and validated questionnaire in both Urdu/English languages. Chi square (χ2) test was used to report any associations between independent variables, i.e., education, type of hospital and work experience and, dependent variables, i.e., pharmacists’ attitudes, experience, and perception. A p-value of ≤0.01 with value of Cramer’s V ≥ 0.3 was considered cut-off for establishing statistical significance. The study was approved by ethical committee and local hospital committees.

Results

Three hundred ninety-six questionnaires were returned out of 500, i.e., response rate = 87.9%. Most participants (92.2%) interacted with doctors at least once daily. Most interactions were related to drug availability inquiry (72.5%). Most pharmacists (91.4%) mentioned that pharmacy duties are mostly clinical in nature. 93.4% of the respondents indicated that pharmacists are reliable source of information regarding general medicines. Furthermore, 87.4% reasoned inadequate training for not being able to discuss issues of clinical nature with doctors.

Conclusion

Pharmacists were willing to perform their duties and provide healthcare benefits to patients however, they seemed sceptical of advanced clinical pharmacy roles such as intervening in prescriptions and medication therapy, consultations and prescribing. There is a need to increase awareness regarding pharmacist’s role. Therefore, it would be helpful if trainings and seminars are conducted on the importance of clinical pharmacy to improve the pharmacy services in Pakistan’s healthcare system.
Profit distribution and managers’ behaviour in vertical integrated medical delivery systems – an experimental economics study
Authors: Bo Yunque , Qi Miaojie , Liu Siyu , Cui Jiyu , Han Youli
Published: July 2020

Background

The vertical integration of medical delivery systems (VIMDSs) is a reform direction both in China and worldwide. We conducted a controlled economic experiment to explore decision-making by managers of medical institutions with respect to profits and what influences the distribution mechanism in VIMDSs.

Methods

Students and hospital staff were recruited to make choices regarding the role of directors of institutions. z-Tree software was used to design the experimental program. Ninety-six subjects participated in the experiment. We gathered 479 valid contracts.

Results

Of the subjects, 66.39% chose flexible contracts. The median of the bidding distribution rate to community health service centres of all auctions was 18.50%. The final distribution rate was approximately 3 percentage points higher than the bidding distribution rate. The median effort level was 9.00. There was a significant correlation between the improvement rate and the choice of effort level (P<0.05) in flexible contracts.

Conclusions

Hospital managers have a preference for flexible contracts because of uncertainty in the medical system. A community health service centre director may behave perfunctorily by engaging in shading in the integration. Flexible contracts and sharing rates beyond the participants’ expectations motivate managers to engage in more cooperative behaviours.
High-dose intranasal application of titanium dioxide nanoparticles induces the systemic uptakes and allergic airway inflammation in asthmatic mice
Authors: Abdulnasser Harfoush Shaza , Hannig Matthias , Le Duc Dung , Heck Sebastian , Leitner Maximilian , Omlor Albert Joachim , Tavernaro Isabella , Kraegeloh Annette , Kautenburger Ralf , Kickelbick Guido , Beilhack Andreas , Bischoff Markus , Nguyen Juliane , Sester Martina , Bals Robert , Dinh Quoc Thai
Published: July 2020

Background

Titanium dioxide nanoparticles (TiO2NPs) have a wide range of applications in several industrial and biomedical domains. Based on the evidence, the workers exposed to inhaled nanosized TiO2powder are more susceptible to the risks of developing respiratory diseases. Accordingly, this issue has increasingly attracted the researchers’ interest in understanding the consequences of TiO2NPs exposure. Regarding this, the present study was conducted to analyze the local effects of TiO2NPs on allergic airway inflammation and their uptake in a mouse model of ovalbumin (OVA)-induced allergic airway inflammation.

Methods

For the purpose of the study, female BALB/c mice with or without asthma were intranasally administered with TiO2NPs. The mice were subjected to histological assessment, lung function testing, scanning electron microscopy (SEM), inductively coupled plasma mass spectrometry (ICP-MS), and NP uptake measurement. In addition, T helper (Th) 1/Th2 cytokines were evaluated in the lung homogenate using the enzyme-linked immunosorbent assay.

Results

According to the results, the mice receiving OVA alone or OVA plus TiO2NPs showed eosinophilic infiltrates and mucus overproduction in the lung tissues, compared to the controls. Furthermore, a significant elevation was observed in the circulating Th2 cytokines, including interleukin (IL)-4, IL-5, and IL-13 after NP exposure. The TiO2NPs were taken up by alveolar macrophages at different time points. As the results of the SEM and ICP-MS indicated, TiO2NPs were present in most of the organs in both asthmatic and non-asthmatic mice.

Conclusion

Based on the findings of the current study, intranasally or inhalation exposure to high-dose nanosized TiO2particles appears to exacerbate the allergic airway inflammation and lead to systemic uptake in extrapulmonary organs. These results indicate the very important need to investigate the upper limit of intranasally or inhalation exposure to nanosized TiO2particles in occupational and environmental health policy.
CDK4/6 inhibition enhances pulmonary inflammatory infiltration in bleomycin-induced lung fibrosis
Authors: Birnhuber Anna , Egemnazarov Bakytbek , Biasin Valentina , Bonyadi Rad Ehsan , Wygrecka Malgorzata , Olschewski Horst , Kwapiszewska Grazyna , Marsh Leigh M.
Published: July 2020

Inhibitors of cyclin-dependent kinases 4/6 (CDK4/6) block cell cycle progression and are commonly used for treatment of several forms of cancer. Due to their anti-proliferative mode of action, we hypothesized that palbociclib could attenuate the development of bleomycin-induced lung fibrosis. In a preclinical setting, mice were treated with bleomycin and then co-treated with or without palbociclib. Lung function, collagen deposition and pulmonary inflammation were analysed after 14 days.

Bleomycin treatment led to an increase of pulmonary fibrosis and inflammation, and concomitant decline of lung function. Palbociclib treatment significantly decreased collagen deposition in the lung after bleomycin treatment, but did not ameliorate lung function. Importantly, palbociclib augmented inflammatory cell recruitment (including macrophages and T cells) in the bronchoalveolar lavage fluid.

This study supports the recent alert from the Food and Drug Administration (FDA) that use of CDK4/6 inhibitors, such as palbociclib, may have severe pulmonary adverse effects. Our study showing heightened pulmonary inflammation following palbociclib treatment highlights the risk of severe inflammatory adverse effects in the lung. This is of special interest in patients with known pulmonary risk factors and emphasizes the need of careful monitoring all patients treated with CDK4/6 inhibitors for signs of lung inflammation.
Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p
Authors: Wu Huijuan , Tian Xin , Zhu Chaoyang
Published: July 2020

Background

Prostate cancer (PCa) greatly threatens men’s lives, with high incidence and mortality. Recently, the research of long non-coding RNAs (lncRNAs) has made breakthroughs in the development of human cancers. This study aimed to figure out the role and action mechanism of lncRNA PVT1 (PVT1) in PCa.

Methods

The expression of PVT1, microRNA-15a-5p (miR-15a-5p) and kinesin family member 23 (KIF23) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, apoptosis, migration and invasion were assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), flow cytometry and transwell assays, respectively. The protein levels of KIF23 and proliferation, apoptosis, and epithelial-mesenchymal transition (EMT)-related markers were quantified by western blot. The relationship between miR-15a-5p and PVT1 or KIF23 was predicted by starBase v2.0 and verified by dual-luciferase reporter assay. Xenograft assay was conducted to determine the role of PVT1 in vivo.

Results

The expression of PVT1 and KIF23 was enhanced, while miR-15a-5p expression was reduced in PCa tissues and cells. PVT1 interference inhibited proliferation, migration and invasion but promoted apoptosis of PCa cells. MiR-15a-5p was a target of PVT1, and KIF23 was a target of miR-15a-5p. The inhibition of miR-15a-5p reversed the effects of PVT1 interference and suppressed the roles of KIF23 knockdown. KIF23 expression was regulated by PVT1 through miR-15a-5p. PVT1 interference blocked PCa progression in vivo.

Conclusion

PVT1 knockdown had effects on the progression of PCa by inhibiting the expression of KIF23 via enriching miR-15a-5p in vitro and in vivo, suggesting that PVT1 might be a novel biomarker for the treatment of PCa.
Oxidative damage mediates the association between polycyclic aromatic hydrocarbon exposure and lung function
Authors: Cao Limin , Zhou Yun , Tan Aijun , Shi Tingming , Zhu Chunmei , Xiao Lili , Zhang Zhuang , Yang Shijie , Mu Ge , Wang Xing , Wang Dongming , Ma Jixuan , Chen Weihong
Published: July 2020

Background

Exposure to polycyclic aromatic hydrocarbons (PAHs) is related to decreased lung function. However, whether oxidative damage is involved in this relationship remains unclear. This study was aimed to explore the potential mediating role of oxidative DNA or lipid damage in the association between PAH exposure and lung function.

Methods

The urinary levels of monohydroxy polycyclic aromatic hydrocarbon metabolites (OH-PAHs) and lung function parameters were measured among 3367 participants from the baseline of the Wuhan-Zhuhai cohort. Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 8-isoprostane (8-iso-PGF2α) were determined to evaluate the individuals’ oxidative DNA and lipid damage degrees, respectively. Linear mixed models were used to investigate the associations of urinary OH-PAHs, 8-OHdG and 8-iso-PGF2α with lung function parameters. Mediation analysis was further conducted to assess the potential role of oxidative damage in the association between urinary OH-PAHs and lung function.

Results

Each one-percentage increase in the sum of urinary OH-PAHs, high-molecular-weight or low-molecular-weight OH-PAHs (ƩOH-PAHs, ƩHMW OH-PAH or ƩLMW OH-PAHs, respectively) was associated with a 0.2152-, 0.2076- or 0.1985- ml decrease in FEV1, and a 0.1891-, 0.2195- or 0.1634- ml decrease in FVC, respectively. Additionally, significantly positive dose-response relationships of ƩOH-PAHs, ƩHMW OH-PAH and ƩLMW OH-PAHs with urinary 8-OHdG or 8-iso-PGF2α, as well as an inverse dose-response relationship between urinary 8-OHdG and FVC, were observed (all P for trend < 0.05). Mediation analysis indicated that urinary 8-OHdG mediated 14.22% of the association between ƩHMW OH-PAH and FVC.

Conclusion

Higher levels of oxidative DNA damage might be involved in the decreased levels of FVC caused by high-molecular-weight PAH exposure.
Development of a national childhood obesogenic environment index in the United States: differences by region and rurality
Authors: Kaczynski Andrew T. , Eberth Jan M. , Stowe Ellen W. , Wende Marilyn E. , Liese Angela D. , McLain Alexander C. , Breneman Charity B. , Josey Michele J.
Published: July 2020

Background

Diverse environmental factors are associated with physical activity (PA) and healthy eating (HE) among youth. However, no study has created a comprehensive obesogenic environment index for children that can be applied at a large geographic scale. The purpose of this study was to describe the development of a childhood obesogenic environment index (COEI) at the county level across the United States.

Methods

A comprehensive search of review articles (n = 20) and input from experts (n = 12) were used to identify community-level variables associated with youth PA, HE, or overweight/obesity for potential inclusion in the index. Based on strength of associations in the literature, expert ratings, expertise of team members, and data source availability, 10 key variables were identified – six related to HE (# per 1000 residents for grocery/superstores, farmers markets, fast food restaurants, full-service restaurants, and convenience stores; as well as percentage of births at baby (breastfeeding)-friendly facilities) and four related to PA (percentage of population living close to exercise opportunities, percentage of population < 1 mile from a school, a composite walkability index, and number of violent crimes per 1000 residents). Data for each variable for all counties in the U.S. (n = 3142) were collected from publicly available sources. For each variable, all counties were ranked and assigned percentiles ranging from 0 to 100. Positive environmental variables (e.g., grocery stores, exercise opportunities) were reverse scored such that higher values for all variables indicated a more obesogenic environment. Finally, for each county, a total obesogenic environment index score was generated by calculating the average percentile for all 10 variables.

Results

The average COEI percentile ranged from 24.5–81.0 (M = 50.02,s.d. = 9.01) across US counties and was depicted spatially on a choropleth map. Obesogenic counties were more prevalent (F = 130.43,p < .0001) in the South region of the U.S. (M = 53.0,s.d. = 8.3) compared to the Northeast (M = 43.2,s.d. = 6.9), Midwest (M = 48.1,s.d. = 8.5), and West (M = 48.4,s.d. = 9.8). When examined by rurality, there were also significant differences (F = 175.86,p < .0001) between metropolitan (M = 46.5,s.d. = 8.4), micropolitan (M = 50.3,s.d. = 8.1), and rural counties (M = 52.9,s.d. = 8.8) across the U.S.

Conclusion

The COEI can be applied to benchmark obesogenic environments and identify geographic disparities and intervention targets. Future research can examine associations with obesity and other health outcomes.
HIV-1 subtype C transmitted founders modulate dendritic cell inflammatory responses
Authors: Lumngwena Evelyn Ngwa , Metenou Simon , Masson Lindi , Cicala Claudia , Arthos James , Woodman Zenda
Published: July 2020

Background

Heterosexual transmission remains the main route of HIV-1 transmission and female genital tract (FGT) inflammation increases the risk of infection. However, the mechanism(s) by which inflammation facilitates infection is not fully understood. In rhesus macaques challenged with simian immunodeficiency virus, dendritic cell (DC) mediated recruitment of CD4+ T cells to the FGT was critical for infection. The aim of this study was to delineate the mechanisms underlying DC-mediated HIV infection by comparing chemokine and pro-inflammatory cytokine production in response to transmitted founder (TF) and chronic infection (CI) Envelope (Env) pseudotyped viruses (PSV).

Results

Monocyte-derived DCs (MDDCs) were stimulated with PSV and recombinant gp140 representing matched TF and CI pairs of four individuals and cytokine secretion measured by multiplex immuno-assay. We found that 4/9 Env induced robust MDDC inflammatory responses and of those, three were cloned from TFs. Overall, TF Env induced MDDCs from healthy donors to secrete higher concentrations of inflammatory cytokines and chemokines than those from CI, suggesting TF Env were better inducers of inflammation. Assessing the signalling pathway associated with inflammatory cytokines, we found that PSV of matched TF and CI variants and a gp140 clone activated ERK and JNK to similar levels. Recombinant soluble DC-SIGN inhibited cytokine release and activation of ERK by PSV, suggesting that Env-DC-SIGN binding was partly involved in MDDC stimulation. Therefore, Env clones might differentially stimulate MDDC immune responses via alternative, yet unidentified signalling pathways.

Conclusion

Overall, this could suggest that the genetics of the virus itself influences inflammatory responses during HIV infection. In the absence of pre-existing infections, induction of greater inflammatory response by TFs might favour virus survival within the healthy FGT by driving an influx of target cells to sites of infection while suppressing immune responses via IL-10.
Predicting reactive stepping in response to perturbations by using a classification approach
Authors: Emmens Amber R. , F. van Asseldonk Edwin H. , Prinsen Vera , der Kooij Herman van
Published: July 2020

Background

People use various strategies to maintain balance, such as taking a reactive step or rotating the upper body. To gain insight in human balance control, it is useful to know what makes people switch from one strategy to another. In previous studies the transition from a non-stepping balance response to reactive stepping was often described by an (extended) inverted pendulum model using a limited number of features. The goal of this study is to predict whether people will take a reactive step to recover from a push and to investigate what features are most relevant for that prediction by using a data-driven approach.

Methods

Ten subjects participated in an experiment in which they received forward pushes to which they had to respond naturally with or without stepping. The collected kinematic and center of pressure data were used to train several classification algorithms to predict reactive stepping. The classification algorithms that performed best were used to determine the most important features through recursive feature elimination.

Results

The neural networks performed better than the other classification algorithms. The prediction accuracy depended on the length of the observation time window: the longer the allowed time between the push and the prediction, the higher the accuracy. Using a neural network with one hidden layer and eight neurons, and a feature set consisting of various kinematic and center of pressure related features, an accuracy of 0.91 was obtained for predictions made up until the moment of step leg unloading, in combination with a sensitivity of 0.79 and a specificity 0.97. The most important features were the acceleration and velocity of the center of mass, and the position of the cervical joint center.

Conclusion

Using our classification-based method the occurrence of reactive stepping could be predicted with a high accuracy, higher than previous methods for predicting natural reactive stepping. The feature set used for that prediction was different from the ones reported in other step prediction studies. Given the high step prediction performance, our method has the potential to be used for triggering reactive stepping in balance controllers of bipedal robots (e.g. exoskeletons).
Statistical measures of motor, sensory and cognitive performance across repeated robot-based testing
Authors: Simmatis Leif E. R. , Early Spencer , Moore Kimberly D. , Appaqaq Simone , Scott Stephen H.
Published: July 2020

Background

Traditional clinical assessments are used extensively in neurology; however, they can be coarse, which can also make them insensitive to change. Kinarm is a robotic assessment system that has been used for precise assessment of individuals with neurological impairments. However, this precision also leads to the challenge of identifying whether a given change in performance reflects a significant change in an individual’s ability or is simply natural variation. Our objective here is to derive confidence intervals and thresholds of significant change for Kinarm Standard Tests™ (KST).

Methods

We assessed participants twice within 15 days on all tasks presently available in KST. We determined the 5–95% confidence intervals for each task parameter, and derived thresholds for significant change. We tested for learning effects and corrected for the false discovery rate (FDR) to identify task parameters with significant learning effects. Finally, we calculated intraclass correlation of type ICC (3,1) (ICC-C) to quantify consistency across assessments.

Results

We recruited an average of 56 participants per task. Confidence intervals for Z-Task Scores ranged between 0.84 and 1.41, and the threshold for significant change ranged between 1.19 and 2.00. We determined that 6/11 tasks displayed learning effects that were significant after FDR correction; these 4 tasks primarily tested cognition or cognitive-motor integration. ICC-C values for Z-Task Scores ranged from 0.29 to 0.70

Conclusions

The present results provide statistical bounds on individual performance for KST as well as significant changes across repeated testing. Most measures of performance had good inter-rater reliability. Tasks with a higher cognitive burden seemed to be more susceptible to learning effects, which should be taken into account when interpreting longitudinal assessments of these tasks.