bluuebunny/crossref_metadata_2025 huggingface dataset:
Patient-specific plate for navigation and fixation of the distal radius: a case series
Authors: Dobbe Johannes G. G. , Peymani Abbas , Roos Hendrika A. L. , Beerens Maikel , Streekstra Geert J. , Strackee Simon D.
Published: February 2021

Purpose

Corrective osteotomy of a malunited distal radius conventionally relies on 2D imaging techniques for alignment planning and evaluation. However, this approach results in suboptimal bone repositioning, which is associated with poor patient outcomes. In this case series, we evaluate the use of novel patient-specific plates (PSPs), which feature navigation and fixation of bone segments as preoperatively planned in 3D.

Methods

Ten participants with distal radius malunion underwent CT scans for preoperative alignment planning. Patient-specific guides and plates were designed, 3D-printed, and sterilized for use in corrective surgery of the distal radius. Pre- and postoperative results were compared in regard to clinical, functional, and radiographic outcomes.

Results

The application of a PSP was successful in 7 of the 10 cases. After treatment, the residual alignment error was reduced by approximately 50% compared with conventional treatment. The use of PSPs reduced pain significantly. Pre- and postoperative results were pooled and demonstrated significant correlations between: (1) pain and malpositioning, (2) the range of pro- and supination motion, the MHOQ score, the EQ-5D-5L score and dorsovolar angulation, and (3) MHOQ score and proximodistal translation.

Conclusion

The correlation between malalignment and MHOQ score, EQ-5D-5L score, pain, and range of motion shows that alignment should be restored as well as possible. Compared to the conventional approach, which relies on 2D imaging techniques, corrective osteotomy based on 3D preoperative planning and intraoperative fixation with a PSP has been shown to improve bone alignment and reduce pain.

Level of evidence

IV.
Chancen und Herausforderungen eines digitalen betrieblichen Gesundheitsmanagements
Authors: Winter Raphaela , Riedl René
Published: February 2021

Hintergrund

Betriebliches Gesundheitsmanagement (BGM) umfasst die Lenkung und Entwicklung betrieblicher Strukturen und Abläufe, um die Arbeit gesundheitsförderlich zu gestalten. Im Zuge der Verbreitung von Informations- und Kommunikationstechnologien wurden in den letzten Jahren vermehrt digitale Tools entwickelt, um die analogen Maßnahmen des BGM zu unterstützen.

Ziel

Zum Einsatz solcher BGM-Tools gibt es bislang noch kaum wissenschaftliche Forschung sowie Erfahrungsberichte. Ziel des vorliegenden Beitrags ist es, die Chancen und Herausforderungen beim Einsatz eines digitalen BGM-Tools aus Unternehmenssicht zu identifizieren.

Material und Methode

Es wurde ein Review durchgeführt, um den Stand der Forschung auf der Basis von Fachliteratur transparent zusammenzufassen. Zudem wurden Experteninterviews durchgeführt, die inhaltsanalytisch ausgewertet wurden. Die Interviews dienten der Validierung der aus dem Review gewonnenen konzeptionell-theoretischen Erkenntnisse.

Ergebnisse und Diskussion

Die durch den Review identifizierten Chancen und Herausforderungen wurden großteils auch von den Expert*innen genannt und somit bestätigt. Das Ansprechen einer breiteren Zielgruppe sowie Zeit- und Kostenersparnisse sind Chancen beim Einsatz von BGM-Tools. Zu den Herausforderungen zählt der Datenschutz sowie die Tatsache, dass die Tools analoge Maßnahmen unterstützen, nicht aber ersetzen können – dies erfordert eine abgestimmte Einsatzstrategie. Auch das Fehlen von standardisierten Kriterien und Gütesiegel ist herausfordernd.
GPR imaging of traffic compaction effects on soil structures
Authors: Akinsunmade Akinniyi
Published: February 2021

Spatial and depth variability of soil characteristics greatly influence its optimum utilization and management. Concealing nature of soil subsurface horizons has made the traditional soil investigations which rely on point information less reliable. In this study, an alternative use of ground penetrating radar (GPR)—a near-surface geophysical survey method—was tested to address the shortcomings. The focus of the study was on assessment of characteristics variability of soil layers at a test site and evaluation of effects of compaction caused by machinery traffics on soil. GPR methods utilize electromagnetic energy in the frequency range of 10 MHz and 3.0 GHz. Fourteen profiles GPR data were acquired at the test site-a farmland in Krakow, Poland. Compaction on parts of the soil was induced using tractor movements (simulating traffic effects) at different passes. Data were processed using basic filtering algorithms and attributes computations executed in Reflexw software. Attempt made in the study was on use of GPR geophysical technique for soil assessment. The method allows delineation of the soil horizons which depicts characteristic depth changes and spatial variability within the horizons. Moreover, traffic effects that caused compaction on parts of the soil horizons were discernable from the GPR profile sections. Thus, similar densification like hardpan that may develop in natural setting can be investigated using the method. The results have shown the suitability of the method for quick, noninvasive and continuous soil investigation that may also allow assessment of temporal soil changes via repeated measurement.
Effectiveness of a game-based class for interdisciplinary energy systems education in engineering courses
Authors: Suzuki Kengo , Shibuya Takeshi , Kanagawa Tetsuya
Published: February 2021

Professionals in the energy sector are required to understand the interactions among technology, society, and the environment to tackle complex trade-offs among policy issues. The fostering of such professionals with interdisciplinary perspectives has been recognized as a significant target for engineering education in universities. Several studies have shown that game-based learning is suitable for teaching interdisciplinary aspects of sustainability-related issues; however, only a few studies have quantitatively evaluated the percentage of participants that learn something relevant to the predetermined learning targets. This study quantitatively evaluates the effectiveness of a game-based class designed to teach energy policy issues in an engineering course. This game-based class aims to develop a perspective for overcoming the complex trade-offs among policy issues and for developing the abilities and attitudes necessary to build society-wide consensus. Content analysis was adopted as a research method; the contents of free-form reflection reports submitted by 128 students were categorized into 6 topics through careful reading and in-depth discussions, and the proportion of students who mentioned topics relevant to the learning targets was calculated. The results show that 83% of the students learned something relevant to the learning targets, and 37% of them made proposals to overcome the trade-offs among policy issues and conflicts among stakeholders. Further, some students actively deepened their understanding through voluntary surveys, presentations of other students, and by comparing the game and reality. These results suggest that game-based learning is an effective method for interdisciplinary education regarding energy policy issues.
Experiences of a “COVID protected” robotic surgical centre for colorectal and urological cancer in the COVID-19 pandemic
Authors: Huddy Jeremy R. , Crockett Matthew , Nizar A Shiyam , Smith Ralph , Malki Manar , Barber Neil , Tilney Henry S.
Published: February 2021

The recent COVID-19 pandemic led to the cancellation of elective surgery across the United Kingdom. Re-establishing elective surgery in a manner that ensures patient and staff safety has been a priority. We report our experience and patient outcomes from setting up a “COVID protected” robotic unit for colorectal and renal surgery that housed both the da Vinci Si (Intuitive, Sunnyvale, CA, USA) and the Versius (CMR Surgical, Cambridge, UK) robotic systems. “COVID protected” robotic surgery was undertaken in a day-surgical unit attached to the main hospital. A standard operating procedure was developed in collaboration with the trust COVID-19 leadership team and adapted to national recommendations. 60 patients underwent elective robotic surgery in the initial 10-weeks of the study. This included 10 colorectal procedures and 50 urology procedures. Median length of stay was 4 days for rectal cancer procedures, 2 days less than prior to the COVID period, and 1 day for renal procedures. There were no instances of in-patient coronavirus transmission. Six rectal cancer patients waited more than 62 days for their surgery because of the initial COVID peak but none had an increase T-stage between pre-operative staging and post-operative histology. Robotic surgery can be undertaken in “COVID protected” units within acute hospitals in a safe way that mitigates the increased risk of undergoing major surgery in the current pandemic. Some benefits were seen such as reduced length of stay for colorectal patients that may be associated with having a dedicated unit for elective robotic surgical services.
Recovery of free volume in PIM-1 membranes through alcohol vapor treatment
Authors: Almansour Faiz , Alberto Monica , Bhavsar Rupesh S. , Fan Xiaolei , Budd Peter M. , Gorgojo Patricia
Published: February 2021

Physical aging is currently a major obstacle for the commercialization of PIM-1 membranes for gas separation applications. A well-known approach to reversing physical aging effects of PIM-1 membranes at laboratory scale is soaking them in lower alcohols, such as methanol and ethanol. However, this procedure does not seem applicable at industrial level, and other strategies must be investigated. In this work, a regeneration method with alcohol vapors (ethanol or methanol) was developed to recover permeability of aged PIM-1 membranes, in comparison with the conventional soaking-in-liquid approach. The gas permeability and separation performance, before and post the regeneration methods, were assessed using a binary mixture of CO2 and CH4 (1:1, v:v). Our results show that an 8-hour methanol vapor treatment was sufficient to recover the original gas permeability, reaching a CO2 permeability > 7000 barrer.
On the local well-posedness of the nonlinear heat equation associated to the fractional Hermite operator in modulation spaces
Authors: Cordero Elena
Published: February 2021

In this note we consider the nonlinear heat equation associated to the fractional Hermite operator $$H^\beta =(-\Delta +|x|^2)^\beta $$

H
β

=

(
-
Δ
+
|
x
|

2

)

β

, $$0<\beta \le 1$$

0
<
β
≤
1

. We show the local solvability of the related Cauchy problem in the framework of modulation spaces. The result is obtained by combining tools from microlocal and time-frequency analysis. As a byproduct, we compute the Gabor matrix of pseudodifferential operators with symbols in the Hörmander class $$S^m_{0,0}$$

S

0
,
0

m

, $$m\in \mathbb {R}$$

m
∈
R

.
Blood Biomarkers for Monitoring and Prognosis of Large Vessel Vasculitides
Authors: Tombetti Enrico , Hysa Elvis , Mason Justin C. , Cimmino Marco A. , Camellino Dario
Published: February 2021

Purpose of Review

Large vessel vasculitides (LVVs) are inflammatory conditions of the wall of large-sized arteries, mainly represented by giant cell arteritis (GCA) and Takayasu arteritis (TA). The inflammatory process within the vessel wall can lead to serious consequences such as development of aneurysms, strokes and blindness; therefore, early diagnosis and follow-up of LVV are fundamental. However, the arterial wall is poorly accessible and blood biomarkers are intended to help physicians not only in disease diagnosis but also in monitoring and defining the prognosis of these conditions, thus assisting therapeutic decisions and favouring personalised management. The field is the object of intense research as the identification of reliable biomarkers is likely to shed light on the mechanisms of disease progression and arterial remodelling. In this review, we will discuss the role of blood biomarkers in LVVs in the light of the latest evidence.

Recent Findings

In clinical practice, the most widely performed laboratory investigations are the erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). However, these indices may be within normal limits during disease relapse and they are not reliable in patients receiving interleukin-6 (IL-6) receptor inhibitors. New biomarkers struggle to gain traction in clinical practice and no molecule with good accuracy has been identified to date. IL-6, a pro-inflammatory cytokine that drives CRP synthesis and increases the ESR, is one of the most promising biomarkers in the field. IL-6 analysis is increasingly performed, and serum levels are more sensitive than ESR for active GCA and might reflect persistent inflammation with high risk of relapse in patients on IL-6 receptor inhibitors. A future with biomarkers that reflect different disease features is an important aspiration. Accordingly, intense effort is being made to identify IL-6-independent inflammatory biomarkers, such as S100 proteins, pentraxin-3 and osteopontin. Moreover, metalloproteinases such as MMP2/9 and angiogenic modulators such as VEGF, YLK-40 and angiopoietins are being studied as markers of arterial remodelling. Lastly, biomarkers indicating organ damage may guide prognostic stratification as well as emergency therapeutic decisions: the most promising biomarkers so far identified are NT-proBNP, which reflects myocardial strain; pentraxin-3, which has been associated with recent optic nerve ischemia; and endothelin-1, which is associated with ischaemic complications.

Currently, the use of these molecules in clinical practice is limited because of their restricted availability, lack of sufficient studies supporting their validity and associated costs. Further evidence is required to better interpret their biological and clinical value.
Association between albuminuria and thyroid function in patients with chronic kidney disease
Authors: Reinhardt Walter , Mülling Nils , Behrendt Stefan , Benson Sven , Dolff Sebastian , Führer Dagmar , Tan Susanne
Published: February 2021

Purpose

The relationship between proteinuria and thyroid function remains controversial in patients with chronic kidney disease (CKD). We prospectively investigated the association between kidney and thyroid function in thyroid antibody-negative patients through all CKD stages.

Methods

We enrolled 184 nondialysis patients (mean age: 63.1 ± 16.9 years) without previous thyroid disease or thyroid-specific antibodies. Kidney function was assessed by estimating the glomerular filtration rate (eGFR) classified according KDIGO (CKD G1–5). Kidney damage was assessed by albuminuria (albumin-to-creatinine ratio, ACR) and classified as mild, moderate, or severe (ACR1: <300, ACR2: 300–3000, and ACR3: 3000 mg/g). To evaluate thyroid function, TSH, T4, fT4, T3, fT3, reverse T3 (rT3), and thyroxine-binding globulin (TBG) were measured.

Results

rT3 concentrations correlated negatively with albuminuria (r = −0.286, p < 0.001) and were significantly lower in patients with severe albuminuria than in those with mild or moderate albuminuria (ACR3: 0.28 vs. ACR2: 0.32 vs. ACR1: 0.36 nmol/l, p < 0.001). The severity of albuminuria revealed no impact on TSH, fT4, T3, fT3, and TBG. EGFR correlated with increasing T4, fT4, T3, fT3, and TBG (T4: r = 0.289, p < 0.01; fT4: r = 0.196, p < 0.01; T3: r = 0.408, p < 0.01; fT3: r = 0.390, p < 0.01) but not with rT3.

Conclusions

In thyroid antibody-negative patients presenting advanced CKD (stages 4 and 5), even severe kidney protein loss failed to influence thyroid hormone status. However, albuminuria severity correlated negatively with rT3, which was significantly lower in patients with albuminuria in the nephrotic range.
The Coding and Small Non-coding Hippocampal Synaptic RNAome
Authors: Epple Robert , Krüger Dennis , Berulava Tea , Brehm Gerrit , Ninov Momchil , Islam Rezaul , Köster Sarah , Fischer Andre
Published: February 2021

Neurons are highly compartmentalized cells that depend on local protein synthesis. Messenger RNAs (mRNAs) have thus been detected in neuronal dendrites, and more recently in the pre- and postsynaptic compartments as well. Other RNA species such as microRNAs have also been described at synapses where they are believed to control mRNA availability for local translation. A combined dataset analyzing the synaptic coding and non-coding RNAome via next-generation sequencing approaches is, however, still lacking. Here, we isolate synaptosomes from the hippocampus of young wild-type mice and provide the coding and non-coding synaptic RNAome. These data are complemented by a novel approach for analyzing the synaptic RNAome from primary hippocampal neurons grown in microfluidic chambers. Our data show that synaptic microRNAs control almost the entire synaptic mRNAome, and we identified several hub microRNAs. By combining the in vivo synaptosomal data with our novel microfluidic chamber system, our findings also support the hypothesis that part of the synaptic microRNAome may be supplied to neurons via astrocytes. Moreover, the microfluidic system is suitable for studying the dynamics of the synaptic RNAome in response to stimulation. In conclusion, our data provide a valuable resource and point to several important targets for further research.
Energy-saving potential for centrifugal pump storage operation using optimized control schemes
Authors: Hieninger Thomas , Goppelt Florian , Schmidt-Vollus Ronald , Schlücker Eberhard
Published: February 2021

In this paper, we present the energy-saving potential of using optimized control for centrifugal pump–driven water storages. For this purpose, a Simulink pump-pipe-storage model is used. The equations and transfer function for steady-state and transient system behavior are presented and verified. Two different control strategies—optimum constant flow rate and level guided speed control—are compared to an allegedly optimal-driven pump with constant rotational speed. Twelve centrifugal pumps between 1 and 120 kW nominal power are evaluated to analyze the influence of different system parameters. The system characteristics, which are the static head, dynamic head factor, and maximum filling head, are varied 25 times for each pump in consideration of the pump’s best efficiency point. Thus, 300 different systems are optimized for each control strategy and compared to the constant speed control. The results are analyzed and the relevant system’s parameters, which have the most significant impact on energy savings, are shown. This theoretical energy–saving potential is verified with measurements, which show the high impact of the part load losses of the frequency converter and the electric motor. A law for identifying and estimation potential energy savings is developed using this information. Four use cases are analyzed with this law. It is shown that for a cost-minimal operation, not only the savings potential but also the operating time is decisive.
Coherent Particle Structures in High-Prandtl-Number Liquid Bridges
Authors: Barmak Ilya , Romanò Francesco , Kannan Parvathy Kunchi , Kuhlmann Hendrik C.
Published: February 2021

Clustering of small rigid spherical particles into particle accumulation structures (PAS) is studied numerically for a high-Prandtl-number (Pr = 68) thermocapillary liquid bridge. The one-way-coupling approach is used for calculation of the particle motion, modeling PAS as an attractor for a single particle. The attractor is created by dissipative forces acting on the particle near the boundary due to the finite size of the particle. These forces can dramatically deflect the particle trajectory from a fluid pathline and transfer it to certain tubular flow structures, called Kolmogorov–Arnold–Moser (KAM) tori, in which the particle is focused and from which it might not escape anymore. The transfer of particles can take place if a KAM torus, which is a property of the flow without particles, enters the narrow boundary layer on the flow boundaries in which the particle experiences extra forces. Since the PAS obtained in this system depends mainly on the finite particle size, it can be classified as a finite-size coherent structure (FSCS).
Complex Tidal Marsh Dynamics Structure Fish Foraging Patterns in the San Francisco Estuary
Authors: Colombano Denise D. , Handley Thomas B. , O’Rear Teejay A. , Durand John R. , Moyle Peter B.
Published: February 2021

Mechanisms driving the consumption and transport of tidal marsh nutrients and energy by fishes are of key interest in the San Francisco Estuary, CA, USA. By combining multiple data sources (gill-net catches, gut contents, channel morphology, tides), we modeled spatial and temporal patterns of fish abundance and gut fullness across a tidal marsh elevation gradient. Channel depth, microhabitat, and tide were important predictors of fish abundance and gut fullness. Species, feeding guild, and season were also important to fish abundance but not to gut fullness, suggesting that abundance was more related to physical constraints of shallow water than to prey availability. Multiple feeding guilds overlapped in space and time at interaction hotspots in subtidal channel habitat near the marsh entrance. In contrast, fish use of shallow intertidal marsh channels was more variable and indicated tradeoffs between foraging and predation. Gut content analysis revealed moderate-to-high gut fullness for all feeding guilds and models predicted high gut fullness in subtidal reaches during tidal flooding, after which fish fed intensively throughout the marsh. While mysids, amphipods, and detritus were common prey among feeding guilds, variation in prey consumption was apparent. Overall, complex tidal marsh hydrogeomorphology driving land-water exchange and residence time may diversify and enhance benthic and pelagic food web pathways to fishes and invertebrates. Furthermore, these findings substantiate the notion that dynamic tidal marshes in this system can support robust secondary production, foraging by multiple feeding guilds, and trophic transfer by fishes to the estuarine mosaic.
Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells
Authors: Zhang Yingzi , Tian Jiao , Qu Chi , Peng Yang , Lei Jinwei , Li Kang , Zong Beige , Sun Lu , Liu Shengchun
Published: February 2021

Background

Recent studies have indicated that serpin peptidase inhibitor, clade A, member 3 (SERPINA3) is a potential marker associated with tumor progression, which connoted that SERPINA3 is related to malignant phenotypes in cancer. However, the biological function of SERPINA3 in breast cancer (BC) remains unclear.

Methods

Bioinformatics data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Immunohistochemical staining (IHC) was conducted to determine SERPINA3 expression. With strong aggressive abilities, triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, BT549 and MDA-MB-436) were obtained to examine SERPINA3 expression and functions. Wound healing and Transwell assays were performed to measure cell migration and invasion. Cell Counting Kit-8 (CCK-8) assay was conducted to detect cell proliferation abilities and cell viabilities.

Results

SERPINA3 was upregulated in BC tissues. Functional assays suggested that overexpression of SERPINA3 significantly promoted cell proliferation, where migration and invasion of TNBC cells were accelerated. Knockdown of SERPINA3 had the opposite effects. These results causing by overexpression of SERPINA3 were also confirmed in non-TNBC cell lines. Overexpression of SERPINA3 remarkably enhanced the epithelial–mesenchymal transition (EMT) by upregulating the EMT markers and EZH2. In addition, the overexpression of SERPINA3 reduced the sensitivity of TNBC cells to cisplatin.

Conclusion

SERPINA3 can regulate the migration, invasion and EMT of TNBC cells and increased expression of SERPINA3 confers resistance to cisplatin in TNBC cells. We discern it is required for the regulation of BC progression and is a critical target for the clinical treatment of BC.
A case of early autoimmune gastritis with characteristic endoscopic findings
Authors: Kishino Maiko , Yao Kenshi , Hashimoto Hiroshi , Nitta Hiroki , Kure Rie , Yamamoto Ayako , Yamamoto Kana , Nonaka Kouichi , Nakamura Shinichi , Tokushige Katsutoshi
Published: February 2021

Significant atrophic gastritis in the fundic gland region is a well-known endoscopic finding observed in autoimmune gastritis (AIG). The endoscopic features of early AIG have not been reported. Iron deficiency, vitamin B12 deficiency, anemia, or neurological symptoms may not be observed in the early stages of AIG, and it may thus be difficult to diagnose early AIG based on clinical findings. We treated a 50-year-old Japanese female whose condition was suspected to be early AIG. The endoscopic findings showed normal gastric pyloric gland mucosa, and diffuse reddened and edematous gastric fundic gland mucosa. Pathologically, local infiltration of lymphocytes and decrease of parietal cells was present in a deep part of the gastric fundic gland mucosa. Blood tests showed that the titer of parietal cell antibody (PCA) was 1:320 and the gastrin level was 820 pg/ml. We determined that the patient had AIG because she also had Hashimoto’s disease, the PCA titer was high, the serum gastrin level was slightly increased, and inflammation was observed only in the gastric body on the endoscopic images. To the best of our knowledge, this is the first case report of endoscopic findings that suggest early AIG, before atrophic changes were observed.
The Fidelity of Training in Behaviour Change Techniques to Intervention Design in a National Diabetes Prevention Programme
Authors: Hawkes Rhiannon E. , Cameron Elaine , Miles Lisa M. , French David P.
Published: February 2021

Background

The National Health Service Diabetes Prevention Programme (NHS-DPP) is a behavioural intervention for people identified as high risk for developing type 2 diabetes that has been rolled out across England. The present study evaluates whether the four commercial providers of the NHS-DPP train staff to deliver behaviour change technique (BCT) content with fidelity to intervention plans.

Method

One set of mandatory training courses across the four NHS-DPP providers (seven courses across 13 days) was audio-recorded, and all additional training materials used were collected. Recordings and training materials were coded for BCT content using the BCT Taxonomy v1. BCTs and depth of training (e.g. instruction, demonstration, practice) of BCT content was checked against providers’ intervention plans.

Results

Ten trainers and 78 trainees were observed, and 12 documents examined. The number of unique BCTs in audio recordings and associated training materials ranged from 19 to 44 across providers, and staff were trained in 53 unique BCTs across the whole NHS-DPP. Staff were trained in 66% of BCTs that were in intervention plans, though two providers trained staff in approximately half of BCTs to be delivered. The most common way that staff were trained in BCT delivery was through instruction. Training delivery style (e.g. experiential versus educational) varied between providers.

Conclusion

Observed training evidences dilution from providers’ intervention plans. NHS-DPP providers should review their training to ensure staff are trained in all key intervention components, ensuring thorough training of BCTs (e.g. demonstrating and practicing how to deliver) to enhance BCT delivery.
Taxon- and senescence-specific fluorescence of colored leaves from the Pliocene Willershausen Lagerstätte, Germany
Authors: Wolkenstein Klaus , Arp Gernot
Published: February 2021

UV-light-induced fluorescence is widely used in the study of coal macerals and palynological samples, but to date has not been described in great detail for plant macrofossils. Here, we report the characteristics of bright UV-light-induced fluorescence of various fossil angiosperm leaf taxa from the Upper Pliocene of Willershausen, Lower Saxony, Germany. The fluorescence is exceptional, since different fluorescence colors ranging from green to yellow to red can be observed and fluorescence properties are found to be related to genera. Using confocal laser scanning microscopy, fluorescence was studied in detail and emission spectra were obtained that allowed to differentiate broad groups of fluorophores. Fluorescence emissions attributed to chlorophyll degradation products demonstrate that fluorescence can be used as an indicator for exceptional chemical preservation of leaf fossils. Comparison with present-day senescing plants suggests that the fluorescence differences in the fossil leaves are mainly caused by taxon-specific degeneration of organic compounds during senescence. The occurrence of various leaf taxa with different fluorescence properties, preserved under identical conditions of fossilization, indicate that diagenesis was not crucial for the differences in leaf fluorescence.
Characterization of immune cell migration using microfabrication
Authors: Vesperini Doriane , Montalvo Galia , Qu Bin , Lautenschläger Franziska
Published: February 2021

The immune system provides our defense against pathogens and aberrant cells, including tumorigenic and infected cells. Motility is one of the fundamental characteristics that enable immune cells to find invading pathogens, control tissue damage, and eliminate primary developing tumors, even in the absence of external treatments. These processes are termed “immune surveillance.” Migration disorders of immune cells are related to autoimmune diseases, chronic inflammation, and tumor evasion. It is therefore essential to characterize immune cell motility in different physiologically and pathologically relevant scenarios to understand the regulatory mechanisms of functionality of immune responses. This review is focused on immune cell migration, to define the underlying mechanisms and the corresponding investigative approaches. We highlight the challenges that immune cells encounter in vivo, and the microfabrication methods to mimic particular aspects of their microenvironment. We discuss the advantages and disadvantages of the proposed tools, and provide information on how to access them. Furthermore, we summarize the directional cues that regulate individual immune cell migration, and discuss the behavior of immune cells in a complex environment composed of multiple directional cues.
Autonomous formation flight using solar radiation pressure
Authors: Thoemel Jan , van Dam Tonie
Published: February 2021

Autonomous formation flight enables new satellite missions for novel applications. The cost and limits of propulsion systems can be overcome if environmental resources are being benefitted of. Currently, atmospheric drag is used in low Earth orbit to this end. Solar radiation pressure, which is of similar order of magnitude as aerodynamic ram pressure, is, however, always neglected. We introduce this force and show that it can be exploited. We demonstrate through simulations that a formation geometry is established quicker if the solar radiation pressure is modeled.
Multilocus gene analysis reveals the presence of two phytoplasma groups in Impatiens balsamina showing flat stem and phyllody
Authors: Panda Priyam , Nigam Amrita , Rao G. P.
Published: February 2021

Rose balsam (Impatiens balsamina) is an important ornamental species grown worldwide for its attractive flowers and also having medicinal properties. Flat stem, little leaf, and phyllody symptoms were observed in I. balsamina nurseries in Uttar Pradesh and Tripura states of India during surveys from 2018 to 2020, with an incidence from 6 to 27%. Amplicons of ~ 1.2 kb were amplified in all the tested symptomatic samples of I. balsamina using universal phytoplasma primer pairs from different surveyed locations, but not from the asymptomatic plants. Pairwise sequence comparison, phylogeny, and virtual RFLP analysis of 16S rRNA gene sequences identified the phytoplasmas as 16SrI-B subgroup strain from Tripura (Lembucherra) and 16SrII-D subgroup strain from Uttar Pradesh (Gorakhpur and Faizabad). Phytoplasma presence and identity was further confirmed by amplifying secA, rp, secY, and tuf genes. This is the first report of 16SrI-B and 16SrII-D phytoplasmas detection in I. balsamina in the world.
A nanoemulsion/micelles mixed nanosystem for the oral administration of hydrophobically modified insulin
Authors: Santalices Irene , Vázquez-Vázquez Carlos , Santander-Ortega Manuel J. , Lozano Victoria , Araújo Francisca , Sarmento Bruno , Shrestha Neha , Préat Veronique , Chenlo Miguel , Alvarez Clara V. , Benetti Federico , Cuñarro Juan , Tovar Sulay , Torres Dolores , Alonso María José
Published: February 2021

The potential of nanoemulsions for the oral administration of peptides is still in its early stage. The aim of the present work was to rationally design, develop, and fully characterize a new nanoemulsion (NE) intended for the oral administration of hydrophobically modified insulin (HM-insulin). Specific components of the NE were selected based on their enhancing permeation properties as well as their ability to improve insulin association efficiency (Miglyol 812, sodium taurocholate), stability in the intestinal fluids, and mucodiffusion (PEGylated phospholipids and poloxamer 407). The results showed that the NE co-existed with a population of micelles, forming a mixed system that exhibited a 100% of HM-insulin association efficiency. The nanosystem showed good stability and miscibility in different bio-relevant media and displayed an acceptable mucodiffusive behavior in porcine mucus. In addition, it exhibited a high interaction with cell mono-cultures (Caco -2 and C2BBe1 human colon carcinoma Caco-2 clone cells) and co-cultures (C2BBe1 human colon carcinoma Caco-2 clone/HT29-MTX cells). The internalization in Caco-2 monolayers was also confirmed by confocal microscopy. Finally, the promising in vitro behavior of the nanosystem in terms of overcoming the biological barriers of the intestinal tract was translated into a moderate, although significant, hypoglycemic response (≈ 20–30%), following intestinal administration to both healthy and diabetic rat models. Overall, this information underlines the crucial steps to address when designing peptide-based nanoformulations to successfully overcome the intestinal barriers associated to the oral modality of administration.

Graphical abstract
Flattening the curve is flattening the complexity of covid-19
Authors: Boumans Marcel
Published: February 2021

Since the February 2020 publication of the article ‘Flattening the curve’ inThe Economist, political leaders worldwide have used this expression to legitimize the introduction of social distancing measures in fighting Covid-19. In fact, this expression represents a complex combination of three components: the shape of the epidemic curve, the social distancing measures and the reproduction number$$ \mathscr{R}_{0}$$R0. Each component has its own history, each with a different history of control. Presenting the control of the epidemic as flattening the curve is in fact flattening the underlying natural-social complexity. The curve that needs to be flattened is presented as a bell-shaped curve, implicitly suggesting that the pathogen’s spread is subject only to natural laws. The$$ \mathscr{R}$$Rvalue, however, is, fundamentally, a metric of how a pathogen behaves within a social context, namely its numerical value is affected by sociopolitical influences. The jagged and erratic empirical curve of Covid-19 illustrates this. Although the virus has most likely infected only a small portion of the total susceptible population, it is clear its shape has changed drastically. This changing shape is largely due to sociopolitical factors. These include shifting formal laws and policies, shifting individual behaviors as well as shifting various other social norms and practices. This makes the course of Covid-19 curve both erratic and unpredictable.
Respirable nano-particulate generations and their pathogenesis in mining workplaces: a review
Authors: Fan Long , Liu Shimin
Published: February 2021

There is a growing concern in mining community about the contribution of nano-particulates to miner’s health. Despite the health influence of respirable dusts and associated lung diseases have been recognized for decades in the mining industry, the nano-scale particulates accompanying with complicated physiochemical properties and their enormous contribution in quantity have been drawing attentions only in recent a few years because of the advancement of nano-science discipline. In this review, we examine the current regulations of dusts exposure and the dominant mass-based monitoring methods to point out the ignorance of nano-particulates in mining industry. The recognized mining-related nano-particulates sources are summarized to identify the mechanically generated finer particulates including particles and aerosols. In addition, the mechanism of adverse health impact on miner with exposure to nano-scale particulates is discussed in a detail to emphasize their substantial detriment as a potential respiratory hazard. Characterization of the complex physiochemical properties of nano-particulates are then summarized and discussed because these properties could be different from regular respirable dusts due to their dramatically increased surface area and particulate counts. The intent of this review is to demonstrate the potential of adverse health effect of nano-particulate on the mine personals throughout the mining cycle and to identify the research gaps of the mine nano-particulate characterization and quantification. We suggest that further understanding of the mining induced nano-particulate properties and their pathogenesis are critical for the future engineering control measure to mitigate the potential health threat for future miners.
Epitaxial Lift-Off of Flexible GaN-Based HEMT Arrays with Performances Optimization by the Piezotronic Effect
Authors: Chen Xin , Dong Jianqi , He Chenguang , He Longfei , Chen Zhitao , Li Shuti , Zhang Kang , Wang Xingfu , Wang Zhong Lin
Published: February 2021

High-electron-mobility transistors (HEMTs) are a promising device in the field of radio frequency and wireless communication. However, to unlock the full potential of HEMTs, the fabrication of large-size flexible HEMTs is required. Herein, a large-sized (> 2 cm2) of AlGaN/AlN/GaN heterostructure-based HEMTs were successfully stripped from sapphire substrate to a flexible polyethylene terephthalate substrate by an electrochemical lift-off technique. The piezotronic effect was then induced to optimize the electron transport performance by modulating/tuning the physical properties of two-dimensional electron gas (2DEG) and phonons. The saturation current of the flexible HEMT is enhanced by 3.15% under the 0.547% tensile condition, and the thermal degradation of the HEMT was also obviously suppressed under compressive straining. The corresponding electrical performance changes and energy diagrams systematically illustrate the intrinsic mechanism. This work not only provides in-depth understanding of the piezotronic effect in tuning 2DEG and phonon properties in GaN HEMTs, but also demonstrates a low-cost method to optimize its electronic and thermal properties.
Ultrathin, Lightweight, and Flexible CNT Buckypaper Enhanced Using MXenes for Electromagnetic Interference Shielding
Authors: Yang Rongliang , Gui Xuchun , Yao Li , Hu Qingmei , Yang Leilei , Zhang Hao , Yao Yongtao , Mei Hui , Tang Zikang
Published: February 2021

Lightweight, flexibility, and low thickness are urgent requirements for next-generation high-performance electromagnetic interference (EMI) shielding materials for catering to the demand for smart and wearable electronic devices. Although several efforts have focused on constructing porous and flexible conductive films or aerogels, few studies have achieved a balance in terms of density, thickness, flexibility, and EMI shielding effectiveness (SE). Herein, an ultrathin, lightweight, and flexible carbon nanotube (CNT) buckypaper enhanced using MXenes (Ti3C2Tx) for high-performance EMI shielding is synthesized through a facile electrophoretic deposition process. The obtained Ti3C2Tx@CNT hybrid buckypaper exhibits an outstanding EMI SE of 60.5 dB in the X-band at 100 μm. The hybrid buckypaper with an MXene content of 49.4 wt% exhibits an EMI SE of 50.4 dB in the X-band with a thickness of only 15 μm, which is 105% higher than that of pristine CNT buckypaper. Furthermore, an average specific SE value of 5.7 × 104 dB cm2 g−1 is exhibited in the 5-μm hybrid buckypaper. Thus, this assembly process proves promising for the construction of ultrathin, flexible, and high-performance EMI shielding films for application in electronic devices and wireless communications.
First record of Pachycrocuta brevirostris (Gervais, 1850) from Ukraine on the background of the European occurrence of the species
Authors: Marciszak Adrian , Semenov Yuriy , Portnicki Piotr , Derkach Tamara
Published: February 2021

Cranial material ofPachycrocuta brevirostrisfrom the late Early Pleistocene site of Nogaisk is the first record of this species in Ukraine. This large hyena was a representative of the Tamanian faunal complex and a single specialised scavenger in these faunas. The revisited European records list ofP.brevirostrisdocumented the presence of this species in 101 sites, dated in the range of 3.5–0.4 Ma. This species first disappeared in Africa, survived in Europe until ca. 0.8–0.7 Ma, and its last, relict occurrence was known from south-eastern Asia. The main reason of extinction ofP.brevirostrisprobably was the competition withCrocuta crocuta. The cave hyena was smaller, but its teeth were proportionally larger to the body size, better adapted to crushing bones and slicing meat, and could also hunt united in larger groups.
rePROBE: Workflow for Revised Probe Assignment and Updated Probe-set Annotation in Microarrays
Authors: Hadlich Frieder , Reyer Henry , Oster Michael , Trakooljul Nares , Muráni Eduard , Ponsuksili Siriluck , Wimmers Klaus
Published: February 2021

Commercial and customized microarrays are valuable tools for the analysis of holistic expression patterns, but require the integration of the latest genomic information. This study provides a comprehensive workflow implemented in an R package (rePROBE) to assign the entire probes and to annotate the probe sets based on up-to-date genomic and transcriptomic information. The rePROBE package can be applied to available gene expression microarray platforms and addresses both public and custom databases. The revised probe assignment and updated probe-set annotation are applied to commercial microarrays available for different livestock species, i.e., chicken (Gallus gallus; ChiGene-1_0-st: 443,579 probes and 18,530 probe sets), pig (Sus scrofa; PorGene-1_1-st: 592,005 probes and 25,779 probe sets), and cattle (Bos Taurus; BovGene-1_0-st: 530,717 probes and 24,759 probe sets), as well as available for human (Homo sapiens; HuGene-1_0-st) and mouse (Mus musculus; HT_MG-430_PM). Using current species-specific transcriptomic information (RefSeq, Ensembl, and partially non-redundant nucleotide sequences) and genomic information, the applied workflow reveals 297,574 probes (15,689 probe sets) for chicken, 384,715 probes (21,673 probe sets) for pig, 363,077 probes (21,238 probe sets) for cattle, 481,168 probes (23,495 probe sets) for human, and 324,942 probes (32,494 probe sets) for mouse. These are representative of 12,641, 15,758, 18,046, 20,167, and 16,335 unique genes that are both annotated and positioned for chicken, pig, cattle, human, and mouse, respectively. Additionally, the workflow collects information on the number of single nucleotide polymorphisms (SNPs) within respective targeted genomic regions and thus provides a detailed basis for comprehensive analyses such as expression quantitative trait locus (eQTL) studies to identify quantitative and functional traits. The rePROBE R package is freely available at https://github.com/friederhadlich/rePROBE.
Compositional Variability and Mutation Spectra of Monophyletic SARS-CoV-2 Clades
Authors: Teng Xufei , Li Qianpeng , Li Zhao , Zhang Yuansheng , Niu Guangyi , Xiao Jingfa , Yu Jun , Zhang Zhang , Song Shuhui
Published: February 2021

COVID-19 and its causative pathogen SARS-CoV-2 have rushed the world into a staggering pandemic in a few months, and a global fight against both has been intensifying. Here, we describe an analysis procedure where genome composition and its variables are related, through the genetic code to molecular mechanisms, based on understanding of RNA replication and its feedback loop from mutation to viral proteome sequence fraternity including effective sites on the replicase-transcriptase complex. Our analysis starts with primary sequence information, identity-based phylogeny based on 22,051 SARS-CoV-2 sequences, and evaluation of sequence variation patterns as mutation spectra and its 12 permutations among organized clades. All are tailored to two key mechanisms: strand-biased and function-associated mutations. Our findings are listed as follows: 1) The most dominant mutation is C-to-U permutation, whose abundant second-codon-position counts alter amino acid composition toward higher molecular weight and lower hydrophobicity, albeit assumed most slightly deleterious. 2) The second abundance group includes three negative-strand mutations (U-to-C, A-to-G, and G-to-A) and a positive-strand mutation (G-to-U) due to DNA repair mechanisms after cellular abasic events. 3) A clade-associated biased mutation trend is found attributable to elevated level of negative-sense strand synthesis. 4) Within-clade permutation variation is very informative for associating non-synonymous mutations and viral proteome changes. These findings demand a platform where emerging mutations are mapped onto mostly subtle but fast-adjusting viral proteomes and transcriptomes, to provide biological and clinical information after logical convergence for effective pharmaceutical and diagnostic applications. Such actions are in desperate need, especially in the middle of the War against COVID-19.
Outcomes of Patients with Thrombocytopenia Evaluated at Hematology Subspecialty Clinics
Authors: Rahman Zaid H. Abdel , Miller Kevin C. , Jabbour Hiba , Alkhatib Yaser , Donthireddy Vijaya
Published: February 2021

Background:

Thrombocytopenia is a frequently encountered laboratory abnormality and a common reason for hematology referrals. Workup for thrombocytopenia is not standardized and frequently does not follow an evidence-based algorithm. We conducted a systematic analysis to evaluate the laboratory testing and outcomes of patients evaluated for thrombocytopenia at hematology clinics in a tertiary referral center between 2013 and 2016.

Patient and methods:

We performed a comprehensive chart review for patients evaluated for thrombocytopenia during the study period. Patients were followed for 1 year from the initial hematology evaluation and assessed for the development of a hematologic malignancy, rheumatologic, or infectious diseases among other clinical outcomes.

Results:

We evaluated 472 patients with a median (range) age of 61 (17–94) years. The majority (63.8%) had mild thrombocytopenia. Within 1 year of follow-up, 14 patients (3.0%) were diagnosed with a hematologic malignancy. A higher likelihood of developing a hematologic malignancy was noted in patients with concurrent leukopenia (hazard ratio [HR] 9.97, 95% confidence interval [CI] 3.28–30.32, p < .01) and increasing age (HR per 10-year deciles 1.52, 95% CI 1.03–2.25, p = .03). In patients with asymptomatic isolated mild thrombocytopenia, laboratory testing did not reveal any significant positive findings and patients did not receive any new major diagnosis during the follow-up period.

Conclusion:

Our findings provide basis and call for development of an evidence-based algorithmic approach for evaluation of patients with thrombocytopenia, testing, and referrals. It also supports a conservative approach mainly driven by physical exam signs, symptoms, and other laboratory findings for patients with isolated mild thrombocytopenia.