bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a Thyroid Hormone Derivative as a Pleiotropic Agent for the Treatment of Alzheimer’s Disease
Authors: Runfola Massimiliano , Perni Michele , Yang Xiaoting , Marchese Maria , Bacci Andrea , Mero Serena , Santorelli Filippo M. , Polini Beatrice , Chiellini Grazia , Giuliani Daniela , Vilella Antonietta , Bodria Martina , Daini Eleonora , Vandini Eleonora , Rudge Simon , Gul Sheraz , Wakelam Michale O. J. , Vendruscolo Michele , Rapposelli Simona
Published: December 2021

The identification of effective pharmacological tools for Alzheimer’s disease (AD) represents one of the main challenges for therapeutic discovery. Due to the variety of pathological processes associated with AD, a promising route for pharmacological intervention involves the development of new chemical entities that can restore cellular homeostasis. To investigate this strategy, we designed and synthetized SG2, a compound related to the thyroid hormone thyroxine, that shares a pleiotropic activity with its endogenous parent compound, including autophagic flux promotion, neuroprotection, and metabolic reprogramming. We demonstrate herein that SG2 acts in a pleiotropic manner to induce recovery in a C. elegans model of AD based on the overexpression of Aβ42 and improves learning abilities in the 5XFAD mouse model of AD. Further, in vitro ADME-Tox profiling and toxicological studies in zebrafish confirmed the low toxicity of this compound, which represents a chemical starting point for AD drug development.
New Heterocyclic Combretastatin A-4 Analogs: Synthesis and Biological Activity of Styryl-2(3H)-benzothiazolones
Authors: Atanasov Gjorgji , Rusew Rusi I. , Gelev Vladimir M. , Chanev Christo D. , Nikolova Rosica , Shivachev Boris L. , Petrov Ognyan I. , Apostolova Margarita D.
Published: December 2021

Here, we describe the synthesis, characterization, and biological activities of a series of 26 new styryl-2(3H)-benzothiazolone analogs of combretastatin-A4 (CA-4). The cytotoxic activities of these compounds were tested in several cell lines (EA.hy926, A549, BEAS-2B, MDA-MB-231, HT-29, MCF-7, and MCF-10A), and the relations between structure and cytotoxicity are discussed. From the series, compound (Z)-3-methyl-6-(3,4,5-trimethoxystyryl)-2(3H)-benzothiazolone (26Z) exhibits the most potent cytotoxic activity (IC50 0.13 ± 0.01 µM) against EA.hy926 cells. 26Z not only inhibits vasculogenesis but also disrupts pre-existing vasculature. 26Z is a microtubule-modulating agent and inhibits a spectrum of angiogenic events in EA.hy926 cells by interfering with endothelial cell invasion, migration, and proliferation. 26Z also shows anti-proliferative activity in CA-4 resistant cells with the following IC50 values: HT-29 (0.008 ± 0.001 µM), MDA-MB-231 (1.35 ± 0.42 µM), and MCF-7 (2.42 ± 0.48 µM). Cell-cycle phase-specific experiments show that 26Z treatment results in G2/M arrest and mitotic spindle multipolarity, suggesting that drug-induced centrosome amplification could promote cell death. Some 26Z-treated adherent cells undergo aberrant cytokinesis, resulting in aneuploidy that perhaps contributes to drug-induced cell death. These data indicate that spindle multipolarity induction by 26Z has an exciting chemotherapeutic potential that merits further investigation.
Medical Device Development for Children and Young People—Reviewing the Challenges and Opportunities
Authors: Dimitri Paul , Pignataro Valeria , Lupo Mariangela , Bonifazi Donato , Henke Maria , Musazzi Umberto M. , Ernst Floris , Minghetti Paola , Redaelli Davide F. , Antimisiaris Sophia G. , Migliaccio Giovanni , Bonifazi Fedele , Marciani Luca , Courtenay Aaron J. , Denora Nunzio , Lopedota Angela
Published: December 2021

Development of specific medical devices (MDs) is required to meet the healthcare needs of children and young people (CYP). In this context, MD development should address changes in growth and psychosocial maturation, physiology, and pathophysiology, and avoid inappropriate repurposing of adult technologies. Underpinning the development of MD for CYP is the need to ensure MD safety and effectiveness through pediatric MD-specific regulations. Contrary to current perceptions of limited market potential, the global pediatric healthcare market is expected to generate around USD 15,984 million by 2025. There are 1.8 billion young people in the world today; 40% of the global population is under 24, creating significant future healthcare market opportunities. This review highlights a number of technology areas that have led to successful pediatric MD, including 3D printing, advanced materials, drug delivery, and diagnostic imaging. To ensure the targeted development of MD for CYP, collaboration across multiple professional disciplines is required, facilitated by a platform to foster collaboration and drive innovation. The European Pediatric Translational Research Infrastructure (EPTRI) will be established as the European platform to support collaboration, including the life sciences industrial sector, to identify unmet needs in child health and support the development, adoption, and commercialization of pediatric MDs.
Giving Oncolytic Viruses a Free Ride: Carrier Cells for Oncolytic Virotherapy
Authors: Reale Alberto , Calistri Arianna , Altomonte Jennifer
Published: December 2021

Oncolytic viruses (OVs) are an emerging class of therapeutics which combine multiple mechanisms of action, including direct cancer cell-killing, immunotherapy and gene therapy. A growing number of clinical trials have indicated that OVs have an excellent safety profile and provide some degree of efficacy, but to date only a single OV drug, HSV-1 talimogene laherparepvec (T-Vec), has achieved marketing approval in the US and Europe. An important issue to consider in order to accelerate the clinical advancement of OV agents is the development of an effective delivery system. Currently, the most commonly employed OV delivery route is intratumoral; however, to target metastatic diseases and tumors that cannot be directly accessed, it is of great interest to develop effective approaches for the systemic delivery of OVs, such as the use of carrier cells. In general, the ideal carrier cell should have a tropism towards the tumor microenvironment (TME), and it must be susceptible to OV infection but remain viable long enough to allow migration and finally release of the OV within the tumor bed. Mesenchymal stem cells (MSCs) have been heavily investigated as carrier cells due to their inherent tumor tropism, in spite of some disadvantages in biodistribution. This review focuses on the other promising candidate carrier cells under development and discusses their interaction with specific OVs and future research lines.
Modeling of High-Density Compaction of Pharmaceutical Tablets Using Multi-Contact Discrete Element Method
Authors: Giannis Kostas , Schilde Carsten , Finke Jan Henrik , Kwade Arno
Published: December 2021

The purpose of this work is to simulate the powder compaction of pharmaceutical materials at the microscopic scale in order to better understand the interplay of mechanical forces between particles, and to predict their compression profiles by controlling the microstructure. For this task, the new framework of multi-contact discrete element method (MC-DEM) was applied. In contrast to the conventional discrete element method (DEM), MC-DEM interactions between multiple contacts on the same particle are now explicitly taken into account. A new adhesive elastic-plastic multi-contact model invoking neighboring contact interaction was introduced and implemented. The uniaxial compaction of two microcrystalline cellulose grades (Avicel® PH 200 (FMC BioPolymer, Philadelphia, PA, USA) and Pharmacel® 102 (DFE Pharma, Nörten-Hardenberg, Germany) subjected to high confining conditions was studied. The objectives of these simulations were: (1) to investigate the micromechanical behavior; (2) to predict the macroscopic behavior; and (3) to develop a methodology for the calibration of the model parameters needed for the MC-DEM simulations. A two-stage calibration strategy was followed: first, the model parameters were directly measured at the micro-scale (particle level) and second, a meso-scale calibration was established between MC-DEM parameters and compression profiles of the pharmaceutical powders. The new MC-DEM framework could capture the main compressibility characteristics of pharmaceutical materials and could successfully provide predictions on compression profiles at high relative densities.
Immunomodulatory and Antioxidant Potential of Biogenic Functionalized Polymeric Nutmeg Oil/Polyurethane/ZnO Bionanocomposite
Authors: Amina Musarat , Al Musayeib Nawal M. , Alarfaj Nawal A. , El-Tohamy Maha F. , Al-Hamoud Gadah A. , Al-yousef Hanan M.
Published: December 2021

The current study is focused on the biosynthesis of nutmeg oil/ polyurethane/ZnONPs bionanocomposite film for immunomodulatory and antioxidant activities. The fabricated film was prepared by using naturally extracted nutmeg oil functionalized with ZnONPs in the presence of polyutherane (PU) medium. The bionanocomposite film was obtained by incorporating dropwise 10 % (w/v) of nutmeg oil to the PU solution/ZnONPs blend. The active constituents of nutmeg oil were determined by gas chromatography coupled with mass spectrometry (GC-MS). The morphological characteristics of the resulting bionanocomposite film were confirmed using various microscopic and spectroscopic methods. Immunomodulatory potential of bionanocomposite was evaluated for RAW 264.7 macrophages. The results exhibited an excellent reduction in inflammatory cytokines (IL-6, IL-10, and TNFα) secretions after the treatment with bionanocomposite. The bionanocomposite exerted the highest inhibitory effects on certain cell signaling constituents that influence the initiation of expression of proinflammatory cytokines. The bionanocomposite was also tested for DPPH and ABTS free radicals scavenging assays and showed excellent antioxidant potential with IC50 values (0.28 ± 0.22 and 0.49 ± 0.36), respectively. The outcomes suggested promising immunomodulatory and antioxidant potentials for the biogenic synthesized nutmeg oil/PU/ZnONPs polymeric bionanocomposite.
Interstitial Arabidopsis-Type Telomeric Repeats in Asteraceae
Authors: Maravilla Alexis J. , Rosato Marcela , Álvarez Inés , Nieto Feliner Gonzalo , Rosselló Josep A.
Published: December 2021

Tandem repeats of telomeric-like motifs at intra-chromosomal regions, known as interstitial telomeric repeats (ITR), have drawn attention as potential markers of structural changes, which might convey information about evolutionary relationships if preserved through time. Building on our previous work that reported outstanding ITR polymorphisms in the genus Anacyclus, we undertook a survey across 132 Asteraceae species, focusing on the six most speciose subfamilies and considering all the ITR data published to date. The goal was to assess whether the presence, site number, and chromosomal location of ITRs convey any phylogenetic signal. We conducted fluorescent in situ hybridization (FISH) using an Arabidopsis-type telomeric sequence as a probe on karyotypes obtained from mitotic chromosomes. FISH signals of ITR sites were detected in species of subfamilies Asteroideae, Carduoideae, Cichorioideae, Gymnarhenoideae, and Mutisioideae, but not in Barnadesioideae. Although six small subfamilies have not yet been sampled, altogether, our results suggest that the dynamics of ITR formation in Asteraceae cannot accurately trace the complex karyological evolution that occurred since the early diversification of this family. Thus, ITRs do not convey a reliable signal at deep or shallow phylogenetic levels and cannot help to delimitate taxonomic categories, a conclusion that might also hold for other important families such as Fabaceae.
The Modulation of Nrf-2/HO-1 Signaling Axis by Carthamus tinctorius L. Alleviates Vascular Inflammation in Human Umbilical Vein Endothelial Cells
Authors: Lee Yun Jung , Lee Yong Pyo , Seo Chang Seob , Choi Eun Sik , Han Byung Hyuk , Yoon Jung Joo , Jang Se Hoon , Jeong Chae Ghang , Mun Yeun Ja , Kang Dae Gill , Lee Ho Sub
Published: December 2021

Carthamus tinctorius L., known as safflower, has been used in traditional treatment for cardiovascular, cerebrovascular, and diabetic vascular complications. We proposed to investigate how the ethanol extract of Carthamus tinctorius L. (ECT) can be used ethnopharmacologically and alleviate vascular inflammatory processes under cytokine stimulation in human vascular endothelial cells. Using the optimized HPLC method, six markers were simultaneously analyzed for quality control of ECT. Pretreatment with ECT (10–100 μg/mL) significantly reduced the increase of leukocyte adhesion to HUVEC by TNF-α in a dose-dependent manner. Cell adhesion molecules (CAMs) such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial cell selectin (E-selectin) are decreased by ECT. In addition, ECT significantly suppressed TNF-α-induced oxidative stress referring to reactive oxygen species (ROS) production. p65 NF-κB nuclear translocation and its activation were inhibited by ECT. Furthermore, pretreatment of ECT increased the HO-1 expression, and nuclear translocation of Nrf-2. These data suggest the potential role of ECT as a beneficial therapeutic herb in vascular inflammation via ROS/NF-kB pathway and the regulation of Nrf-2/HO-1 signaling axis is involved in its vascular protection. Thus, further study will be needed to clarify which compound is dominant for protection of vascular diseases.
Development of SSR Databases Available for Both NGS and Capillary Electrophoresis in Apple, Pear and Tea
Authors: Nishio Sogo , Kunihisa Miyuki , Taniguchi Fumiya , Kajiya-Kanegae Hiromi , Moriya Shigeki , Takeuchi Yukie , Sawamura Yutaka
Published: December 2021

Developing new varieties in fruit and tea breeding programs is very costly and labor-intensive. Thus, establishing a variety discrimination system is important for protecting breeders’ rights and producers’ profits. Simple sequence repeat (SSR) databases that can be utilized for both next-generation sequencing (SSR-GBS) and polymerase chain reaction–capillary electrophoresis (PCR-CE) would be very useful in variety discrimination. In the present study, SSRs with tri-, tetra- and pentanucleotide repeats were examined in apple, pear and tea. Out of 37 SSRs that showed clear results in PCR-CE, 27 were suitable for SSR-GBS. Among the remaining markers, there was allele dropout for some markers that caused differences between the results of PCR-CE and SSR-GBS. For the selected 27 markers, the alleles detected by SSR-GBS were comparable to those detected by PCR-CE. Furthermore, we developed a computational pipeline for automated genotyping using SSR-GBS by setting a value “α” for each marker, a criterion whether a genotype is homozygous or heterozygous based on allele frequency. The set of 27 markers contains 10, 8 and 9 SSRs for apple, pear and tea, respectively, that are useful for both PCR-CE and SSR-GBS and suitable for automation. The databases help researchers discriminate varieties in various ways depending on sample size, markers and methods.
Genome Size, Cytotype Diversity and Reproductive Mode Variation of Cotoneaster integerrimus (Rosaceae) from the Balkans
Authors: Bogunić Faruk , Siljak-Yakovlev Sonja , Mahmutović-Dizdarević Irma , Hajrudinović-Bogunić Alma , Bourge Mickaël , Brown Spencer C. , Muratović Edina
Published: December 2021

Cotoneaster integerrimus represents a multiploid and facultative apomictic system of widely distributed mountain populations. We used flow cytometry to determine genome size, ploidy level, and reproduction mode variation of the Balkan populations, supplemented by analysis of nuclear microsatellites in order to address: (i) geographic distribution and variation of cytotypes among the populations; (ii) variation of reproduction mode and the frequency of sexuality; (iii) pathways of endosperm formation among the sampled polyploids and their endosperm balance requirements; (iv) genotypic diversity and geographic distribution of clonal lineages of polyploids. The prevalence of apomictic tetraploid cytotype followed by sexual diploids and extremely rare triploids was demonstrated. This prevalence of tetraploids affected the populations’ structure composed from clonal genotypes with varying proportions. The co-occurrence of diploids and tetraploids generated higher cytotype, reproductive mode, and genotypic diversity, but mixed-ploidy sites were extremely rare. The endosperm imbalance facilitates the development and the occurrence of intermediate triploids in mixed-ploidy populations, but also different tetraploid lineages elsewhere with unbalanced endosperm. All these results showed that the South European populations of C. integerrimus have higher levels of cytotype and reproductive diversity compared to the Central European ones. Therefore, the South European populations can be considered as a potential reservoir of regional and global diversity for this species.
In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators
Authors: Blinstrubienė Aušra , Jančauskienė Inga , Burbulis Natalija
Published: December 2021

Miscanthus x giganteus is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of Miscanthus x giganteus and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L–1 IBA + 0.1 mg L–1 BAP + 4.0 mg L–1 l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L–1 ZEA + 1.0 mg L–1 NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L–1 IBA, where more than 95% of plant regenerants survived and were growing normally.
Multicomponent Polyphenolic Extracts from Vaccinium corymbosum at Lab and Pilot Scale. Characterization and Effectivity against Nosocomial Pathogens
Authors: Gato Eva , Perez Astrid , Rosalowska Alicja , Celeiro Maria , Bou German , Lores Marta
Published: December 2021

An extraction method was designed and scaled up to produce multicomponent polyphenolic extracts from blueberries (Vaccinium corymbosum) of three different varieties. The process was specifically drawn up to comply with green chemistry principles. Extracts were obtained for the direct assessment of their antimicrobial and antiadhesive activities, and their direct use in the control of infections caused by concerning multidrug-resistant nosocomial pathogens. Analytical characterization was performed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Similar qualitative profiles were obtained in the three studied varieties with some significant quantitative differences. Up to 22 different polyphenols were identified with a clear predominance of anthocyani(di)ns followed by flavanols, non-flavonoids, and far behind by flavan-3-ols and procyanidins. The individual content of the main polyphenols was also discussed. A pilot scale extract has been also produced as a proof-of-concept, showing that scaling-up triples the content of bioactive phytochemicals. The effect of the polyphenolic extracts was analyzed against seven multidrug-resistance bacterial species by performing biofilm formation and growth and killing curves assays. All the studied varieties showed antibacterial and antiadhesive activities, being the extract containing the highest concentration of bioactive polyphenols, the most active with a high bactericidal effect.
Metamitron, a Photosynthetic Electron Transport Chain Inhibitor, Modulates the Photoprotective Mechanism of Apple Trees
Authors: Tadmor Yuval , Raz Amir , Reikin-Barak Shira , Ambastha Vivek , Shemesh Eli , Leshem Yehoram , Crane Omer , Stern Raphael , Goldway Martin , Tchernov Dan , Liran Oded
Published: December 2021

Chemical thinning of apple fruitlets is an important practice as it reduces the natural fruit load and, therefore, increases the size of the final fruit for commercial markets. In apples, one chemical thinner used is Metamitron, which is sold as the commercial product Brevis® (Adama, Ashdod, Israel). This thinner inhibits the electron transfer between Photosystem II and Quinone-b within light reactions of photosynthesis. In this study, we investigated the responses of two apple cultivars—Golden Delicious and Top Red—and photosynthetic light reactions after administration of Brevis®. The analysis revealed that the presence of the inhibitor affects both cultivars’ energetic status. The kinetics of the photoprotective mechanism’s sub-processes are attenuated in both cultivars, but this seems more severe in the Top Red cultivar. State transitions of the antenna and Photosystem II repair cycle are decreased substantially when the Metamitron concentration is above 0.6% in the Top Red cultivar but not in the Golden Delicious cultivar. These attenuations result from a biased absorbed energy distribution between photochemistry and photoprotection pathways in the two cultivars. We suggest that Metamitron inadvertently interacts with photoprotective mechanism-related enzymes in chloroplasts of apple tree leaves. Specifically, we hypothesize that it may interact with the kinases responsible for the induction of state transitions and the Photosystem II repair cycle.
Development of a Low-Cost System for 3D Orchard Mapping Integrating UGV and LiDAR
Authors: Murcia Harold F. , Tilaguy Sebastian , Ouazaa Sofiane
Published: December 2021

Growing evaluation in the early stages of crop development can be critical to eventual yield. Point clouds have been used for this purpose in tasks such as detection, characterization, phenotyping, and prediction on different crops with terrestrial mapping platforms based on laser scanning. 3D model generation requires the use of specialized measurement equipment, which limits access to this technology because of their complex and high cost, both hardware elements and data processing software. An unmanned 3D reconstruction mapping system of orchards or small crops has been developed to support the determination of morphological indices, allowing the individual calculation of the height and radius of the canopy of the trees to monitor plant growth. This paper presents the details on each development stage of a low-cost mapping system which integrates an Unmanned Ground Vehicle UGV and a 2D LiDAR to generate 3D point clouds. The sensing system for the data collection was developed from the design in mechanical, electronic, control, and software layers. The validation test was carried out on a citrus crop section by a comparison of distance and canopy height values obtained from our generated point cloud concerning the reference values obtained with a photogrammetry method. A 3D crop map was generated to provide a graphical view of the density of tree canopies in different sections which led to the determination of individual plant characteristics using a Python-assisted tool. Field evaluation results showed plant individual tree height and crown diameter with a root mean square error of around 30.8 and 45.7 cm between point cloud data and reference values.
Light-Mediated Reduction in Photosynthesis in Closed Greenhouses Can Be Compensated for by CO2 Enrichment in Tomato Production
Authors: Dannehl Dennis , Kläring Hans-Peter , Schmidt Uwe
Published: December 2021

Concepts of semi-closed greenhouses can be used to save energy, whereas their technical equipment often causes a decrease in the light received by the plants. Nevertheless, higher yields are achieved, which are presumably triggered by a higher CO2 concentration in the greenhouse and associated higher photosynthesis because of the technical cooling and the longer period of closed ventilation. Therefore, we examined the effects of photosynthetic photon flux density (PPFD) and CO2 concentration on plant photosynthesis and transpiration in tomato using a multiple cuvette gas exchange system. In a growth chamber experiment, we demonstrated that a light-mediated reduction in photosynthesis can be compensated or even overcompensated for by rising CO2 concentration. Increasing the CO2 concentration from 400 to 1000 µmol mol−1 within the PPFD range from 303 to 653 µmol m−2 s−1 resulted in an increase in net photosynthesis of 51%, a decrease in transpiration of 5 to 8%, and an increase in photosynthetic water use efficiency of 60%. Estimations showed that light reductions of 10% can be compensated for via increasing the CO2 concentration by about 100 µmol mol−1 and overcompensated for by about 40% if CO2 concentration is kept at 1000 instead of 400 µmol mol−1.
Effects of NaCl on Antioxidant, Antifungal, and Antibacterial Activities in Safflower Essential Oils
Authors: Attia Houneida , Harrathi Jamel , Alamer Khalid H. , Alsalmi Fatin A. , Magné Christian , Khalil Maha
Published: December 2021

The present study aims to evaluate the antioxidant and antimicrobial activity of essential oils (EO) extracted from safflower plants grown in the absence and presence of NaCl, 50 mM. Plants treated with 50 mM of NaCl showed decreases in root, stem, and leaf dry weight. Results of the essential oils showed that roots have a higher EO yield than leaves and stems. Salinity caused a decrease in this yield in roots and leaves but not in stems. The compounds identified in the EO extracted from these organs belong to seven chemical classes of which the dominant class is the sesquiterpene hydrocarbons. The chemotype of C. tinctorius EO is variable depending on the organ and the treatment. The safflower essential oils showed low antioxidant, antiradical, and iron-reducing activities compared to those of the positive control (BHT). In an antifungal activity test, only two strains, Aspergillus niger and Candida albicans, were found to be highly sensitive to these oils as they showed almost total inhibition of their growth. For antibacterial activity, safflower EOs showed significant antimicrobial activity against Bacillus subtilis, Bacillus cereus, and Xanthomonas campestris in both control and NaCl-treated plants: for these three strains, total inhibition of growth was noted at 50,000 ppm of EO in leaves and roots; whereas for stems, total inhibition was noted only for the third strain (Xanthomonas campestris). For other strains, this inhibition was variable and weak. Salt was found to have no effect on the activities of safflower EOs.
Oxidative Stress and DNA Lesion Reduction of a Polyphenolic Enriched Extract of Thymus marschallianus Willd. in Endothelial Vascular Cells Exposed to Hyperglycemia
Authors: Ielciu Irina , Filip Gabriela Adriana , Oniga Ilioara , Olah Neli-Kinga , Bâldea Ioana , Olteanu Diana , Burtescu Ramona Flavia , Turcuș Violeta , Sevastre-Berghian Alexandra C. , Benedec Daniela , Hanganu Daniela
Published: December 2021

The present study aimed to compare two polyphenolic-enriched extracts obtained from the Thymus marschallianus Willd. (Lamiaceae) species, harvested from culture (TMCE in doses of 0.66 μg GAE/mL and 0.066 μg GAE/mL) and from spontaneous flora (TMSE in doses of 0.94 μg GAE/mL and 0.094 μg GAE/mL) by assessing their biological effects on human umbilical vein endothelial cells (HUVECs) exposed to normoglycemic (137 mmol/L glucose) and hyperglycemic conditions (200 mmol/L glucose). Extracts were obtained by solid phase extraction (SPE) and analyzed by chromatographical (HPLC-DAD) and spectrophotometrical methods. Their effects on hyperglycemia were evaluated by the quantification of oxidative stress and NF-ĸB, pNF-ĸB, HIF-1α, and γ-H2AX expressions. The HPLC-DAD analysis highlighted significant amounts of rosmarinic acid (ranging between 0.18 and 1.81 mg/g dry extract), luteolin (ranging between 2.04 and 17.71 mg/g dry extract), kaempferol (ranging between 1.85 and 7.39 mg/g dry extract), and apigenin (ranging between 4.97 and 65.67 mg/g dry extract). Exposure to hyperglycemia induced oxidative stress and the activation of NF-ĸ increased the expression of HIF-1α and produced DNA lesions. The polyphenolic-enriched extracts proved a significant reduction of oxidative stress and γ-H2AX formation and improved the expression of HIF-1α, suggesting their protective role on endothelial cells in hyperglycemia. The tested extracts reduced the total NF-ĸB expression and diminished its activation in hyperglycemic conditions. The obtained results bring evidence for the use of the polyphenolic-enriched extracts of T. marschallianus as adjuvants in hyperglycemia.
Biogeographical Patterns of Herbivore Arthropods Associated with Chenopodium quinoa Grown along the Latitudinal Gradient of Chile
Authors: Chorbadjian Rodrigo A. , Ahumada María I. , Urra Francisco , Elgueta Mario , Gilligan Todd M.
Published: December 2021

Identifying the particular guilds of herbivore arthropods that affect the production of crops is key to developing sustainable pest-management strategies; however, there is incomplete information about the identity of herbivore arthropods that could potentially damage the production of both highland and lowland quinoa landraces grown in Chile. By both reviewing the literature and conducting field collections across a large latitudinal gradient, we generated an updated list of 43 herbivore arthropods associated with quinoa production in Chile. In general, most species are polyphagous feeders, and only seven are specialists. The number and identity of species varied in relation with the latitude, such that four distinctive assemblages of herbivores were identified, each containing 32, 27, 34, and 22 species between latitudes 18–26, 26–32, 32–40, and 40–44° S, respectively. The most northern production area (18–26° S) is affected by nine unique species, including the major quinoa pest Eurysacca quinoae Povolný (Lepidoptera: Gelechiidae). Similarly, the central area (32–40° S) contains four unique species, including Eurysacca media Povolný (Lepidoptera: Gelechiidae) and Orthotylus flavosparsus (Sahlberg) (Hemiptera: Miridae). The particular species assemblages described here will help further development of local pest-management practices.
Selection Gain of Maize Haploid Inducers for the Tropical Savanna Environments
Authors: Dermail Abil , Chankaew Sompong , Lertrat Kamol , Lübberstedt Thomas , Suriharn Khundej
Published: December 2021

Lacking elite haploid inducers performing high haploid induction rate (HIR) and agronomic performance is one of fundamental factors hindering the rapid adoption of doubled haploid technology in maize hybrid breeding, especially under tropical savanna climate. Breeding haploid inducers for specific agro-ecology, thus, is indispensable yet challenging. We used temperate inducer Stock6 as genetic source for haploid induction ability and eight tropical maize genotypes as principal donors for agronomic adaptation. Three cycles of modified ear-to-row with 5% intra-family selection were applied in a population set of 78 putative haploid inducer families emphasized on agronomic performance, R1-nj anthocyanin intensity, and inducer seed set. Genetic gains, variance components, and heritability on given traits were estimated. Hierarchical clustering based on five selection criteria was performed to investigate the phenotypic diversity of putative families. Cycle effect was predominant for all observed traits. Realized genetic gain was positive for HIR (0.40% per cycle) and inducer seed set (30.10% or 47.30 seeds per ear per cycle). In this study, we reported the first haploid inducers for regions under tropical savanna climate. Three inducer families, KHI-42, KHI-54, and KHI-64, were promising as they possessed HIR about 7.8% or 14 haploid seeds per tester ear and inducer seed rate about 95.0% or 208 inducer seeds per ear. The breeding method was effective for enhancing the seed set and the expression of R1-nj anthocyanin marker of inducers, yet it showed a low effectiveness to improve haploid induction rate. Introgression of temperate inducer Stock6 into tropical gene pool followed by phenotypic selections through modified ear-to-row selection on inducer seed set and R1-nj marker did not compromise the agronomic traits of tropical inducer families. Implications and further strategies for optimizing genetic gain on HIR are discussed.
Vulnerability of the Ancient Peat Plateaus in Western Siberia
Authors: Pastukhov Alexander , Marchenko-Vagapova Tatiana , Loiko Sergey , Kaverin Dmitry
Published: December 2021

Based on the data of the plant macrofossil and palynological composition of the peat deposits, the evolution and current state of polygonal peatlands were analyzed at the southern limit of continuous permafrost in the Pur-Taz interfluve. Paleoreconstruction shows that peat accumulation began in the Early Holocene, about 9814 cal. year BP, in the Late Pre-Boreal (PB-2), at a rate of 1 to 1.5 mm year−1. Intensive peat accumulation continued in the Boreal and early Atlantic. The geocryological complex of polygonal peatlands has remained a stable bog system despite the predicted warming and increasing humidity. However, a rather rapid upper permafrost degradation and irreversible changes in the bog systems of polygonal peatlands occur with anthropogenic disturbances, in particular, a change in the natural hydrological regime under construction of linear objects.
Sewage Pollution Promotes the Invasion-Related Traits of Impatiens glandulifera in an Oligotrophic Habitat of the Sharr Mountain (Western Balkans)
Authors: Stanojevic Milos , Trailovic Maja , Dubljanin Tijana , Krivošej Zoran , Nikolic Miroslav , Nikolic Nina
Published: December 2021

An annual plant, Himalayan balsam (Impatiens glandulifera Royle) is globally widespread and one of Europe’s top invaders. We focused on two questions: does this species indeed not invade the southern areas and does the environment affect some of its key invisibility traits. In an isolated model mountainous valley, we jointly analyzed the soil (21 parameters), the life history traits of the invader (height, stem diameter, aboveground dw), and the resident vegetation (species composition and abundances, Ellenberg indicator values), and supplemented it with local knowledge (semi-structured interviews). Uncontrolled discharge of fecal wastewaters directly into the local dense hydrological network fostered mass infestation of an atypical habitat. The phenotypic plasticity of the measured invasion-related traits was very high in the surveyed early invasion (30–50% invader cover) stages. Different microhabitat conditions consistently correlated with its growth performance. The largest individuals were restricted to the deforested riparian habitats, with extreme soil nutrient enrichment (primarily by P and K) and low-competitive, species-poor resident vegetation. We showed that ecological context can modify invasion-related traits and what could affect a further invasion process. Finally, this species is likely underreported in the wider region; public attitude and loss of traditional ecological knowledge are further management risks.
Prospects for Protective Potential of Moringa oleifera against Kidney Diseases
Authors: Akter Tanzina , Rahman Md Atikur , Moni Akhi , Apu Md. Aminul Islam , Fariha Atqiya , Hannan Md. Abdul , Uddin Md Jamal
Published: December 2021

Kidney diseases are regarded as one of the major public health issues in the world. The objectives of this study were: (i) to investigate the causative factors involved in kidney disease and the therapeutic aspects of Moringa oleifera, as well as (ii) the effectiveness of M. oleifera in the anti-inflammation and antioxidant processes of the kidney while minimizing all potential side effects. In addition, we proposed a hypothesis to improve M. oleifera based drug development. This study was updated by searching the key words M. oleifera on kidney diseases and M. oleifera on oxidative stress, inflammation, and fibrosis in online research databases such as PubMed and Google Scholar. The following validation checking and scrutiny analysis of the recently published articles were used to explore this study. The recent existing research has found that M. oleifera has a plethora of health benefits. Individual medicinal properties of M. oleifera leaf extract, seed powder, stem extract, and the whole extract (ethanol/methanol) can up-increase the activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), while decreasing the activity of inflammatory cytokines such as TNF-α, IL-1β, IL-6, and COX-2. In our study, we have investigated the properties of this plant against kidney diseases based on existing knowledge with an updated review of literature. Considering the effectiveness of M. oleifera, this study would be useful for further research into the pharmacological potential and therapeutic insights of M. oleifera, as well as prospects of Moringa-based effective medicine development for human benefits.
Photophysical Properties of Linked Zinc Phthalocyanine to Acryloyl Chloride:N-vinylpyrrolidone Copolymer
Authors: Potlog Tamara , Lungu Ion , Tiuleanu Pavel , Robu Stefan
Published: December 2021

This paper focuses on the linking of zinc phthalocyanine (ZnPc) to N-vinylpyrrolidone (N-VP): acryloyl chloride (ClAC) copolymer. The synthesis of binary N-VP:ClAC copolymer was performed by the radical polymerization method and then grafted to ZnPc by the Friedel Crafts acylation reaction. We have developed a water-soluble ZnPc:ClAC:N-VP photosensitizer with a narrow absorption band at 970 nm, fluorescence at λem = 825 nm and the decay fluorescence profile with 3-decay relatively longer times of 1.2 µs, 4.6 µs, and 37 µs. The concentration-dependent dark cytotoxicity investigated in normal fibroblasts (NHDF), malignant melanoma (MeWo), adenocarcinoma (HeLa), and hepatocellular carcinoma (HepG2) cell lines incubated to increased concentrations of ZnPc:ClAC:N-VP (up to 40 μM) for 24 h in the dark show low cytotoxicity. Maximum cell viability in HeLa and HepG2 tumor cell lines was observed.
Deformation of Poly-l-lactid acid (PLLA) under Uniaxial Tension and Plane-Strain Compression
Authors: Vozniak Alina , Bartczak Zbigniew
Published: December 2021

The ability of PLLA, either amorphous or semicrystalline, to plastic deformation to large strain was investigated in a wide temperature range (Td = 70–140 °C). Active deformation mechanisms have been identified and compared for two different deformation modes—uniaxial drawing and plane-strain compression. The initially amorphous PLLA was capable of significant deformation in both tension and plane-strain compression. In contrast, the samples of crystallized PLLA were found brittle in tensile, whereas they proved to be ductile and capable of high-strain deformation when deformed in plane-strain compression. The main deformation mechanism identified in amorphous PLLA was the orientation of chains due to plastic flow, followed by strain-induced crystallization occurring at the true strain above e = 0.5. The oriented chains in amorphous phase were then transformed into oriented mesophase and/or oriented crystals. An upper temperature limit for mesophase formation was found below Td = 90 °C. The amount of mesophase formed in this process did not exceed 5 wt.%. An additional mesophase fraction was generated at high strains from crystals damaged by severe deformation. After the formation of the crystalline phase, further deformation followed the mechanisms characteristic for the semicrystalline polymer. Interlamellar slip supported by crystallographic chain slip has been identified as the major deformation mechanism in semicrystalline PLLA. It was found that the contribution of crystallographic slip increased notably with the increase in the deformation temperature. The most probable active crystallographic slip systems were (010)[001], (100)[001] or (110)[001] slip systems operating along the chain direction. At high temperatures (Td = 115–140 °C), the α→β crystal transformation was additionally observed, leading to the formation of a small fraction of β crystals.
Development of Dental Poly(methyl methacrylate)-Based Resin for Stereolithography Additive Manufacturing
Authors: Hata Kentaro , Ikeda Hiroshi , Nagamatsu Yuki , Masaki Chihiro , Hosokawa Ryuji , Shimizu Hiroshi
Published: December 2021

Poly(methyl methacrylate) (PMMA) is widely used in dental applications. However, PMMA specialized for stereolithography (SLA) additive manufacturing (3D-printing) has not been developed yet. This study aims to develop a novel PMMA-based resin for SLA 3D-printing by mixing methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), and PMMA powder in various mixing ratios. The printability and the viscosity of the PMMA-based resins were examined to determine their suitability for 3D-printing. The mechanical properties (flexural strength and Vickers hardness), shear bond strength, degree of conversion, physicochemical properties (water sorption and solubility), and cytotoxicity for L929 cells of the resulting resins were compared with those of three commercial resins: one self-cured resin and two 3D-print resins. EGDMA and PMMA were found to be essential components for SLA 3D-printing. The viscosity increased with PMMA content, while the mechanical properties improved as EGDMA content increased. The shear bond strength tended to decrease as EGDMA increased. Based on these characteristics, the optimal composition was determined to be 30% PMMA, 56% EGDMA, 14% MMA with flexural strength (84.6 ± 7.1 MPa), Vickers hardness (21.6 ± 1.9), and shear bond strength (10.5 ± 1.8 MPa) which were comparable to or higher than those of commercial resins. The resin’s degree of conversion (71.5 ± 0.7%), water sorption (19.7 ± 0.6 μg/mm3), solubility (below detection limit), and cell viability (80.7 ± 6.2% at day 10) were all acceptable for use in an oral environment. The printable PMMA-based resin is a potential candidate material for dental applications.
Development of a Weighted Barite-Free Formate Drilling Mud for Well Construction under Complicated Conditions
Authors: Morenov Valentin , Leusheva Ekaterina , Liu Tianle
Published: December 2021

Construction of oil and gas wells at offshore fields often involves high formation pressure and the presence of swellable clay rocks in the section. In addition, productivity preservation is also an important aspect. For this purpose, it is necessary to reduce the solids content of the drilling mud. The purpose of this work is to develop, improve, and study compositions of weighted drilling muds with low content of solids, on the basis of organic salts of alkali metals and polymers for the construction of wells prone to rock swelling and/or cavings, as well as drilling fluids for drilling-in the formation. In order to achieve the set goal the following is required: Analysis of existing drilling muds of higher density for drilling wells in unstable rock intervals and for drilling in the productive formation; analysis of experience in using drilling systems on the formic acid salts base and substantiation of requirements for flushing fluids during well construction; development and investigation of drilling mud compositions on the formate base; and the evaluation of inhibiting effect of systems containing organic salts, polymer reagents, and calcium carbonate on clay samples. The developed drilling mud is characterized by a high inhibiting ability that allows minimized mud-weighting by the natural solid phase. This reduces the volume of prepared mud and facilitates the regulation of its properties by reducing the dispersion of drilled cuttings; it eliminates problems related to hydration and the swelling of active clay rocks; and stabilizes unstable argillites prone to caving. The low solids content, low filtration rates, and inhibitory nature of the mud allows high stability of the rheological properties of the mud, and preserves oil and gas reservoir productivity under conditions of elevated formation pressure.
Clergy Perceptions of Mental Illness and Confronting Stigma in Congregations
Authors: Campbell Anthony David
Published: December 2021

Mental illness and stigma are key concerns in congregations and represent important threats to community health. Clergies are considered influential in how congregants think about and respond to mental health issues, especially in African American congregations. In-depth interviews with 32 African American and White clergies were conducted to understand their unique perspectives on mental health and how they interact with their congregations based on those perspectives. Findings include six themes related to mental health stigma, namely, holistic definitions of health; African Americans and different conceptions of mental health (only reported by African American clergies); code words and language; depression as a special case; perceptions of mental health counseling and treatment; and clergy strategies for addressing mental health stigma. The clergies in this study recognized their influence on ideas related to mental health in their congregations, and most expressed active efforts toward discussing mental health and reducing stigma.
The Stalin Cult as Political Religion
Authors: Gill Graeme
Published: December 2021

Political religion is a concept that gained prominence around the middle of the twentieth century, being associated for many with the idea of a totalitarian regime. Political religion was seen as a secular ideology whose followers took it up with the enthusiasm and commitment normally associated with adherence to religion. Comprising liturgy, ritual and the sacralization of politics, it created a community of believers, and usually had a transcendental leadership and a millennial vision of a promised future. This paper will explore the utility of this concept for understanding leader cults in authoritarian regimes. Such cults have been prominent features of authoritarian regimes but there is little agreement at the conceptual level about how they should be understood. One of the most powerful of such cults was that of Joseph Stalin in the Soviet Union from 1929 to 1953. This paper analyses this cult in terms of liturgy and ritual and concludes that despite some aspects that are common between the cult and religion, most ritualistic aspects of religion find no direct counterpart in the cult.
Sur-Sangam and Punjabi Zabur (Psalms 24:7–10): Messianic and Missiological Perspectives in the Indian Subcontinent
Authors: Sarwar Eric
Published: December 2021

How does the local raga-based music setting of Psalm 24:7–10 become associated with Christian identity in an Islamic context? How does Psalm 24 strengthen the faith of the marginalized church and broaden messianic hope? In what ways does Psalm 24:7–10 equip local Christians for missional engagement? This paper focuses on the convergence of the local raga-based musical concept of sur-sangam and the revealed text of Punjabi Psalms/Zabur 24:7–10. It argues that while poetic translated text in Punjabi vernacular remains a vital component of theological pedagogy, local music expresses the emotional voice that (re)assures of the messianic hope and mandates missional engagement in Pakistan. Throughout the convergence, musical, messianic, and missional perspectives are transformed to a local phenomenon and its practice is perceived in a cross-cultural connection. Furthermore, examining the text and tune of Punjabi Zabur (Psalms) 24:7–10 in the Indo-Pak context may stretch the spectrum of religious repertoire in the contemporary intercultural world.