bluuebunny/crossref_metadata_2025 huggingface dataset:
A Versatile MANET Experimentation Platform and Its Evaluation through Experiments on the Performance of Routing Protocols under Diverse Conditions
Authors: Manolopoulos Ioannis , Loukatos Dimitrios , Kontovasilis Kimon
Published: May 2022

Mobile Ad hoc Networks (MANETs) are characterized by highly dynamic phenomena and volatility. These features have a significant impact on network performance and should be present in the scenarios of experiments for the assessment of MANET-related technologies. However, the currently available experimentation approaches suffer from limitations, either employing overly abstract simulation-based models that cannot capture real-world imperfections or drawing upon “monolithic” testbeds suited only to a narrow set of predetermined technologies, operational scenarios, or environmental conditions. Toward addressing these limitations, this work proposes a versatile platform that can accommodate many of the complexities present in real-world scenarios while still remaining highly flexible and customizable to enable a wide variety of MANET-related experiments. The platform is characterized by a modular architecture with clearly defined modules for the signaling between peer mobile nodes, the tracking of each node’s location and motion, the routing protocol functionality, and the management of communication messages at each node. The relevant software runs on inexpensive Raspberry Pi-based commodity hardware, which can be readily attached to robotic devices for moving the network nodes in accordance with controlled mobility patterns. Moreover, through an appropriate tuning of certain modules, a number of important operational conditions can be precisely controlled through software, e.g., restricting the communications range (thus reducing the network density) or for emulating the mobility patterns of nodes. The effectiveness and versatility of the proposed platform are demonstrated through the realization of a series of experiments on the performance comparison of selected routing protocols under diverse network density conditions.
The Stance4Health Project: Evaluating a Smart Personalised Nutrition Service for Gut Microbiota Modulation in Normal- and Overweight Adults and Children with Obesity, Gluten-Related Disorders or Allergy/Intolerance to Cow’s Milk
Authors: Dello Russo Marika , Russo Paola , Rufián-Henares José Ángel , Hinojosa-Nogueira Daniel , Pérez-Burillo Sergio , de la Cueva Silvia Pastoriza , Rohn Sascha , Fatouros Alexandra , Douros Konstantinos , González-Vigil Verónica , Epstein David , Francino M. Pilar , Siani Alfonso , Lauria Fabio
Published: May 2022

Unhealthy diets represent a major risk for the pathogenesis of metabolic and chronic inflammatory diseases. Improving the quality of diet is important to prevent chronic diseases, and diet-induced modifications of the gut microbiota (GM) community likely play an important role. The EU-funded Stance4Health project aims at performing a randomized clinical trial based on a nutritional intervention program in the context of normal weight and overweight adults as well as children with obesity and gluten-related disorders or allergy/intolerance to cow’s milk. The trial will evaluate the efficacy of a Smart Personalised Nutrition (SPN) service in modifying GM composition and metabolic function and improving consumer empowerment through technology adoption.
Development, Validation and Application of an Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry (UHPLC-MS/MS) Method after QuEChERS Cleanup for Selected Dichloroanilines and Phthalates in Rice Samples
Authors: Tsochatzis Emmanouil , Begou Olga , Kalogiannis Stavros , Gika Helen , Oz Emel , Oz Fatih , Theodoridis Georgios
Published: May 2022

Dichloroanilines and phthalic acid esters (phthalates) are food contaminants, stable in solution even at high temperatures, which exhibit considerable toxic effects, while acting as endocrine disruptors. In the present study, a quick and easy UHPLC-MS/MS method for simultaneously analyzing two dichloroanilines (3,4-DCA and 3,5-DCA) and six phthalates (DMP, DnBP, BBP, DnOP, DEHP, and mBP) in commercial rice samples was developed, validated, and applied. For the cleanup process, the methodology of quick, easy, cheap, effective, rugged, and safe (QuEChERS) was applied, whereas different dispersants (GCB, C18, and PSA) were tested. What was developed and presented had limits of detection ranging from 0.017 up to 0.12 mg/kg, recoveries (trueness) below 120%, and relative standard deviations (RSD; precision) <15% for all target analytes, whilst no significant matrix effects occurred for all analytes. It was determined that the rice samples analyzed using this developed technique did not contain any of the two dichloroaniline compounds (3,4-DCA and 3,5-DCA) nor two of the six phthalate (DMP and mBP) compounds analyzed, while the levels of other phthalates (DEHP, BBP, DnBP and DnOP) were within the legal limits. The current method ensures a fast and easy approach for the high-throughput quantification of the selected food contaminants in rice.
How Different Are Industrial, Artisanal and Homemade Soft Breads?
Authors: Maurice Bastien , Saint-Eve Anne , Pernin Aurélia , Leroy Pascal , Souchon Isabelle
Published: May 2022

Soft bread has a significant relevance in modern diets, and its nutritional impact on human health can be substantial. Within this product category, there is an extensive range of ingredients, formulations, and processing methods, which all contribute to the vast diversity found in the final products. This work compared the impact of three different processing methods (industrial, artisanal, and homemade preparation) on the technological (formulation and processing, as they are interconnected in real-life conditions), nutritional, and physicochemical properties of soft bread. In total, 24 types of soft bread were analyzed: 10 industrial, 6 artisanal, and 8 homemade. Although production diagrams were similar among the three methods, industrial recipes contained on average more ingredients and more additives. Industrial bread was lower in saturated fat compared to the other two groups, but contained more sugar than homemade bread. The physical properties of all loaves were comparable, with the exception of higher crumb elasticity in industrial bread compared to homemade. An analysis of volatile molecules revealed more lipid oxidation markers in industrial bread, more fermentation markers in artisanal bread, and fewer markers of Maillard reactions in homemade bread. Chemical reactions during processing seem to be the principal criterion making possible to discriminate the different processing methods. These results offer a quantitative assessment of the differences within a single product category, reflecting the real-world choices for consumers.
Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
Authors: Zhu Wenbo , Zhao Lingyan , Jin Jingyi , Song Yang
Published: May 2022

Under the excitation of a 980 nm excitation light, the fluorescence signals of the synthesized core-shell NaYF4:Yb@NaYF4:Ho and monolayer NaYF4:Yb,Tm upconversion nanoparticles (UCNPs) were simultaneously detected at 656 and 696 nm, respectively. The two upconversion materials were coupled with anti-clothianidin and anti-imidacloprid monoclonal antibodies by the glutaraldehyde cross-linking method as signal probes. Imidacloprid (IMI) and clothianidin (CLO) could compete with antigen-conjugated amino Fe3O4 magnetic nanomaterials for binding to signaling probes, thus establishing a rapid and sensitive fluorescent immunoassay for the simultaneous detection of IMI and CLO. Under optimal conditions, the limits of detection (LOD, IC10) and sensitivity (IC50) of IMI and CLO were (0.032, 0.028) and (4.7, 2.1) ng/mL, respectively, and the linear assay ranges were at 0.032–285.75 ng/mL and 0.028–200 ng/mL, respectively. Immunoassay did not cross-react significantly with other analogs. In fruits and vegetables such as apples, oranges, peaches, cucumbers, tomatoes and peppers, the mean recoveries of IMI and CLO ranged from 83.33% to 115.02% with relative standard deviations (RSDs) of 1.9% to 9.2% and 1.2% to 9.0%, respectively. Furthermore, the results of the immunoassay correlate well with the high-performance liquid chromatography method used to detect the actual samples.
The Preparation of Novel P(OEGMA-co-MEO2MA) Microgels-Based Thermosensitive Hydrogel and Its Application in Three-Dimensional Cell Scaffold
Authors: Liu Yang , Luo Yu-Ning , Zhang Pei , Yang Wen-Fei , Zhang Cai-Yao , Yin Yu-Li
Published: May 2022

Thermosensitive hydrogel scaffolds have attracted particular attention in three-dimensional (3D) cell culture. It is very necessary to develop a type of thermosensitive hydrogel material with low shrinkage, and excellent biocompatibility and biodegradability. Here, five types of thermosensitive microgels with different volume phase transition temperature (VPTT) or particle sizes were first synthesized using 2-methyl-2-propenoic acid-2-(2-methoxyethoxy) ethyl ester (MEO2MA) and oligoethylene glycol methyl ether methacrylate (OEGMA) as thermosensitive monomers by free radical polymerization. Their VPTT and particle sizes were investigated by a nanometer particle size meter and an ultraviolet spectrophotometer. The feasibility of using these P(OEGMA-co-MEO2MA) microgels to construct thermosensitive hydrogel by means of the thermal induction method is discussed for the first time. The prepared thermosensitive hydrogel with the optimum performance was screened for in situ embedding and three-dimensional (3D) culture of MCF-7 breast cancer cells. The experimental results of AO/EB and MTT methods indicate that the pioneering scaffold material has prominent biocompatibility, and cells grow rapidly in the 3D scaffold and maintain high proliferative capacity. At the same time, there is also a tendency to aggregate to form multicellular spheres. Therefore, this original P(OEGMA-co-MEO2MA) thermosensitive hydrogel can serve as a highly biocompatible and easily functionalized 3D cell culture platform with great potential in the biomedical area.
Potential Targets for Deprescribing in Medically Complex Older Adults with Suspected Cognitive Impairment
Authors: Pavon Juliessa M. , Berkowitz Theodore S. Z. , Smith Valerie A. , Hughes Jaime M. , Hung Anna , Hastings Susan N.
Published: May 2022

Deprescribing may be particularly beneficial in patients with medical complexity and suspected cognitive impairment (CI). We describe central nervous system (CNS) medication use and side effects in this population and explore the relationship between anticholinergic burden and sleep. We conducted a cross-sectional analysis of baseline data from a pilot randomized-controlled trial in older adult veterans with medical complexity (Care Assessment Need score > 90), and suspected CI (Telephone Interview for Cognitive Status score 20–31). CNS medication classes included antipsychotics, benzodiazepines, H2-receptor antagonists, hypnotics, opioids, and skeletal muscle relaxants. We also coded anticholinergic-active medications according to their Anticholinergic Cognitive Burden (ACB) score. Other measures included self-reported medication side effects and the Pittsburgh Sleep Quality Index (PSQI). ACB association with sleep (PSQI) was examined using adjusted linear regression. In this sample (N = 40), the mean number of prescribed CNS medications was 2.2 (SD 1.5), 65% experienced ≥ 1 side effect, and 50% had an ACB score ≥ 3 (high anticholinergic exposure). The ACB score ≥ 3 compared to ACB < 3 was not significantly associated with PSQI scores (avg diff in score = −0.1, 95% CI −2.1, 1.8). Although results did not demonstrate a clear relationship with worsened sleep, significant side effects and anticholinergic burden support the deprescribing need in this population.
Decolonizing Brazil through Science Fiction: Bacurau and Brazilian Empowerment
Authors: Zuin Lidia
Published: May 2022

The objective of this article is to present an overview of the changes in Brazilian science fiction throughout the past decades, which culminated in the international and national success of the film Bacurau and the emergence of the new subgenres amazofuturismo and sertãopunk in continuation of Causo’s tupinipunk. These subgenres are bringing to the plate topics such as nationalism, decolonization, racism, social justice, and globalization, while using pop formulas and, thus, engaging an audience that is finding in those titles an opportunity to address the current political scenario. This paper suggests that Bacurau, amazofuturismo, and sertãopunk are new manifestations that are paving the way for contemporary Brazilian artists, who are seeking to overcome what Rodrigues’ called “the mongrel complex”, by updating certain proposals already made by tupinipunk in the 1990s. As the country celebrates the 100th anniversary of the Modern Art Week, this article proposes that contemporary Brazilian science fiction is echoing contemporary humanities agenda topics, such as decoloniality and politics of recognition, in response to the past four years of Jair Bolsonaro’s government.
Identification of Determinants of Biofeedback Treatment’s Efficacy in Treating Migraine and Oxidative Stress by ARIANNA (ARtificial Intelligent Assistant for Neural Network Analysis)
Authors: Ciancarelli Irene , Morone Giovanni , Tozzi Ciancarelli Maria Giuliana , Paolucci Stefano , Tonin Paolo , Cerasa Antonio , Iosa Marco
Published: May 2022

Migraines are a public health problem that impose severe socioeconomic burdens and causes related disabilities. Among the non-pharmacological therapeutic approaches, behavioral treatments such as biofeedback have proven effective for both adults and children. Oxidative stress is undoubtedly involved in the pathophysiology of migraines. Evidence shows a complex relationship between nitric oxide (NO) and superoxide anions, and their modification could lead to an effective treatment. Conventional analyses may fail in highlighting the complex, nonlinear relationship among factors and outcomes. The aim of the present study was to verify if an artificial neural network (ANN) named ARIANNA could verify if the serum levels of the decomposition products of NO—nitrite and nitrate (NOx)—the superoxide dismutase (SOD) serum levels, and the Migraine Disability Assessment Scores (MIDAS) could constitute prognostic variables predicting biofeedback’s efficacy in migraine treatment. Twenty women affected by chronic migraine were enrolled and underwent an EMG-biofeedback treatment. The results show an accuracy for the ANN of 75% in predicting the post-treatment MIDAS score, highlighting a statistically significant correlation (R = −0.675, p = 0.011) between NOx (nitrite and nitrate) and MIDAS only when the peroxide levels in the serum were within a specific range. In conclusion, the ANN was proven to be an innovative methodology for interpreting the complex biological phenomena and biofeedback treatment in migraines.
Genetic Diversity and Genome-Wide Association Study of Architectural Traits of Spray Cut Chrysanthemum Varieties
Authors: Sun Daojin , Zhang Luyao , Su Jiangshuo , Yu Qi , Zhang Jiali , Fang Weimin , Wang Haibin , Guan Zhiyong , Chen Fadi , Song Aiping
Published: May 2022

The architecture of spray cut chrysanthemum is crucial for the quality and quantity of cut flower production. However, the mechanism underlying plant architecture still needs to be clarified. In this study, we measured nine architecture-related traits of 195 spray cut chrysanthemum varieties during a two-year period. The results showed that the number of upper primary branches, number of lateral flower buds and primary branch length widely varied. Additionally, plant height had a significant positive correlation with number of leaf nodes and total number of lateral buds. Number of upper primary branches had a significant negative correlation with primary branch diameter, primary branch angle and primary branch length. Plant height, total number of lateral buds, number of upper primary branches, stem diameter, primary branch diameter and primary branch length were vulnerable to environmental impacts. All varieties could be divided into five categories according to cluster analysis, and the typical plant architecture of the varieties was summarized. Finally, a genome-wide association study (GWAS) was performed to find potential functional genes.
Enhanced Feature Pyramid Vision Transformer for Semantic Segmentation on Thailand Landsat-8 Corpus
Authors: Intarat Kritchayan , Rakwatin Preesan , Panboonyuen Teerapong
Published: May 2022

Semantic segmentation on Landsat-8 data is crucial in the integration of diverse data, allowing researchers to achieve more productivity and lower expenses. This research aimed to improve the versatile backbone for dense prediction without convolutions—namely, using the pyramid vision transformer (PRM-VS-TM) to incorporate attention mechanisms across various feature maps. Furthermore, the PRM-VS-TM constructs an end-to-end object detection system without convolutions and uses handcrafted components, such as dense anchors and non-maximum suspension (NMS). The present study was conducted on a private dataset, i.e., the Thailand Landsat-8 challenge. There are three baselines: DeepLab, Swin Transformer (Swin TF), and PRM-VS-TM. Results indicate that the proposed model significantly outperforms all current baselines on the Thailand Landsat-8 corpus, providing F1-scores greater than 80% in almost all categories. Finally, we demonstrate that our model, without utilizing pre-trained settings or any further post-processing, can outperform current state-of-the-art (SOTA) methods for both agriculture and forest classes.
No Significant Bone Resorption after Open Treatment of Mandibular Condylar Head Fractures in the Medium-Term
Authors: Neuhaus Michael-Tobias , Gellrich Nils-Claudius , Sander Anna Katharina , Lethaus Bernd , Halama Dirk , Zimmerer Rüdiger M.
Published: May 2022

Open treatment of condylar head fractures (CHF) is considered controversial. In this retrospective cohort study our primary objective was therefore to assess bone resorption and remodeling as well as patients function after open treatment of CHF in a medium-term follow-up (15.1 ± 2.2 months). We included 18 patients with 25 CHF who underwent open reduction and internal fixation, between 2016 and 2021, in our analysis. The clinical data and cone-beam computed tomography (CBCT) datasets were analyzed. The condylar processes were segmented in the postoperative (T1) and follow-up (T2) CBCT scans. Volumetric and linear bone changes were the primary outcome variables, measured by using a sophisticated 3D-algorithm. The mean condylar head volume decreased non-significantly from 3022.01 ± 825.77 mm3 (T1) to 2878.8 ± 735.60 mm3 (T2; p = 0.52). Morphological alterations indicated remodeling and resorption. The pre-operative maximal interincisal opening (MIO) was 19.75 ± 3.07 mm and significantly improved to 40.47 ± 1.7 mm during follow-up (p = 0.0005). Low rates of postoperative complications were observed. Open reduction of CHF leads to good clinical outcomes and low rates of medium-term complications. This study underlines the feasibility and importance of open treatment of CHF and may help to spread its acceptance as the preferred treatment option.
The Importance of Arterial Stiffness Assessment in Patients with Familial Hypercholesterolemia
Authors: Kovács Beáta , Cseprekál Orsolya , Diószegi Ágnes , Lengyel Szabolcs , Maroda László , Paragh György , Harangi Mariann , Páll Dénes
Published: May 2022

Cardiovascular diseases are still the leading cause of mortality due to increased atherosclerosis worldwide. In the background of accelerated atherosclerosis, the most important risk factors include hypertension, age, male gender, hereditary predisposition, diabetes, obesity, smoking and lipid metabolism disorder. Arterial stiffness is a firmly established, independent predictor of cardiovascular risk. Patients with familial hypercholesterolemia are at very high cardiovascular risk. Non-invasive measurement of arterial stiffness is suitable for screening vascular dysfunction at subclinical stage in this severe inherited disorder. Some former studies found stiffer arteries in patients with familial hypercholesterolemia compared to healthy controls, while statin treatment has a beneficial effect on it. If conventional drug therapy fails in patients with severe familial hypercholesterolemia, PCSK9 inhibitor therapy should be administered; if these agents are not available, performing selective LDL apheresis could be considered. The impact of recent therapeutic approaches on vascular stiffness is not widely studied yet, even though the degree of accelerated athero and arteriosclerosis correlates with cardiovascular risk. The authors provide an overview of the diagnosis of familial hypercholesterolemia and the findings of studies on arterial dysfunction in patients with familial hypercholesterolemia, in addition to presenting the latest therapeutic options and their effects on arterial elasticity parameters.
Impaired Left Ventricular Circumferential Midwall Systolic Performance Appears Linked to Depressed Preload, but Not Intrinsic Contractile Dysfunction or Excessive Afterload, in Paradoxical Low-Flow/Low-Gradient Severe Aortic Stenosis
Authors: Długosz Dorota , Surdacki Andrzej , Zawiślak Barbara , Bartuś Stanisław , Chyrchel Bernadeta
Published: May 2022

Paradoxical low-flow/low-gradient aortic stenosis (P-LFLG-AS) occurs in about one-third of patients with severe AS and preserved left ventricular (LV) ejection fraction (EF). Our aim was to differentiate between altered LV loading conditions and contractility as determinants of subtle LV systolic dysfunction in P-LFLG-AS. We retrospectively analyzed medical records of patients with isolated severe degenerative AS and preserved EF (30 subjects with P-LFLG-AS and 30 patients with normal-flow/high-gradient severe AS (NFHG-AS)), without relevant coexistent diseases (e.g., diabetes, coronary artery disease and chronic kidney disease) or any abnormalities which could account for a low-flow state. Patients with P-LFLG-AS and NFHG-AS did not differ in aortic valve area index and most clinical characteristics. Compared to NFHG-AS, subjects with P-LFLG-AS exhibited smaller LV end-diastolic diameter (LVd) (44 ± 5 vs. 54 ± 5 mm, p < 0.001) (consistent with lower LV preload) with pronounced concentric remodeling, higher valvulo-arterial impedance (3.8 ± 1.1 vs. 2.2 ± 0.5 mmHg per mL/m2, p < 0.001) and diminished systemic arterial compliance (0.45 ± 0.11 vs. 0.76 ± 0.23 mL/m2 per mmHg, p < 0.001), while circumferential end-systolic LV midwall stress (cESS), an estimate of afterload at the LV level, was similar in P-LFLG-AS and NFHG-AS (175 ± 83 vs. 198 ± 69 hPa, p = 0.3). LV midwall fractional shortening (mwFS) was depressed in P-LFLG-AS vs. NFHG-AS (12.3 ± 3.5 vs. 14.7 ± 2.9%, p = 0.006) despite similar EF (61 ± 6 vs. 59 ± 8%, p = 0.4). By multiple regression, the presence of P-LFLG-AS remained a significant predictor of lower mwFS compared to NFHG-AS upon adjustment for cESS (β ± SEM: −2.35 ± 0.67, p < 0.001); however, the significance was lost after further correction for LVd (β = −1.10 ± 0.85, p = 0.21). In conclusion, the association of P-LFLG-AS with a lower cESS-adjusted mwFS, an index of afterload-corrected LV circumferential systolic function at the midwall level, appears secondary to a smaller LV end-diastolic cavity size according to the Frank–Starling law. Thus, low LV preload, not intrinsic contractile dysfunction or excessive afterload, may account for impaired LV circumferential midwall systolic performance in P-LFLG-AS.
Anti-Disgust Cognitive Behavioral Therapy for Contamination-Based Obsessive Compulsive Disorder: A Randomized Controlled Clinical Trial
Authors: Salmani Behzad , Mancini Francesco , Hasani Jafar , Zanjani Zahra
Published: May 2022

Background: Disgust is a strong and persistent emotion that frequently occurs during exposure-based treatments for contamination-based obsessive compulsive disorder (C-OCD). This study aimed to examine the efficacy of augmenting cognitive behavioral therapy (CBT) with a novel type of anti-disgust cognitive intervention in reducing the severity of OCD, disgust propensity/sensitivity, and refusal rate of exposure and response prevention, while simultaneously increasing acceptance of disgust. Materials and Methods: Fifty-five individuals with C-OCD (mean age 28.1 years, SD = 3.52; 77% female) were randomly assigned to 15 weekly sessions of anti-disgust plus CBT (AD-CBT) or CBT alone. They were evaluated for outcomes four times (pretreatment, prior to exposure and response prevention (ERP) sessions, posttreatment, and three-month follow-up), and mixed-design ANOVAs were used to analyze the data. Results: The findings indicated that when compared to CBT alone, AD-CBT significantly reduced OCD severity, disgust propensity/sensitivity, and concurrently increased disgust acceptance (p < 0.001). Additionally, engaging in an anti-disgust cognitive intervention was associated with lower ERP refusal rate (4% vs. 16%). The superiority of AD-CBT over CBT persisted through the three-month follow-up period. Conclusions: The current study suggests that supplementing CBT for C-OCD with an anti-disgust cognitive intervention significantly increased acceptance of disgust and decreased the refusal rate of ERP, OCD severity, and disgust-related factors.
Prediction of the Short-Term Therapeutic Effect of Anti-VEGF Therapy for Diabetic Macular Edema Using a Generative Adversarial Network with OCT Images
Authors: Xu Fabao , Liu Shaopeng , Xiang Yifan , Hong Jiaming , Wang Jiawei , Shao Zheyi , Zhang Rui , Zhao Wenjuan , Yu Xuechen , Li Zhiwen , Yang Xueying , Geng Yanshuang , Xiao Chunyan , Wei Min , Zhai Weibin , Zhang Ying , Wang Shaopeng , Li Jianqiao
Published: May 2022

Purpose: To generate and evaluate individualized post-therapeutic optical coherence tomography (OCT) images that could predict the short-term response of anti-vascular endothelial growth factor (VEGF) therapy for diabetic macular edema (DME) based on pre-therapeutic images using generative adversarial network (GAN). Methods: Real-world imaging data were collected at the Department of Ophthalmology, Qilu Hospital. A total of 561 pairs of pre-therapeutic and post-therapeutic OCT images of patients with DME were retrospectively included in the training set, 71 pre-therapeutic OCT images were included in the validation set, and their corresponding post-therapeutic OCT images were used to evaluate the synthetic images. A pix2pixHD method was adopted to predict post-therapeutic OCT images in DME patients that received anti-VEGF therapy. The quality and similarity of synthetic OCT images were evaluated independently by a screening experiment and an evaluation experiment. Results: The post-therapeutic OCT images generated by the GAN model based on big data were comparable to the actual images, and the response of edema resorption was also close to the ground truth. Most synthetic images (65/71) were difficult to differentiate from the actual OCT images by retinal specialists. The mean absolute error (MAE) of the central macular thickness (CMT) between the synthetic OCT images and the actual images was 24.51 ± 18.56 μm. Conclusions: The application of GAN can objectively demonstrate the individual short-term response of anti-VEGF therapy one month in advance based on OCT images with high accuracy, which could potentially help to improve treatment compliance of DME patients, identify patients who are not responding well to treatment and optimize the treatment program.
Effect of Serious Gaming on Speech-in-Noise Intelligibility in Adult Cochlear Implantees: A Randomized Controlled Study
Authors: Reynard Pierre , Attina Virginie , Idriss Samar , Hermann Ruben , Barilly Claire , Veuillet Evelyne , Joly Charles-Alexandre , Thai-Van Hung
Published: May 2022

Listening in noise remains challenging for adults with cochlear implants (CI) even after prolonged experience. Personalized auditory training (AT) programs can be proposed to improve specific auditory skills in adults with CI. The objective of this study was to assess serious gaming as a rehabilitation tool to improve speech-in-noise intelligibility in adult CI users. Thirty subjects with bilateral profound hearing loss and at least 9 months of CI experience were randomized to participate in a 5-week serious game-based AT program (n = 15) or a control group (n = 15). All participants were tested at enrolment and at 5 weeks using the sentence recognition-in-noise matrix test to measure the signal-to-noise ratio (SNR) allowing 70% of speech-in-noise understanding (70% speech reception threshold, SRT70). Thirteen subjects completed the AT program and nine of them were re-tested 5 weeks later. The mean SRT70 improved from 15.5 dB to 11.5 dB SNR after 5 weeks of AT (p < 0.001). No significant change in SRT70 was observed in the control group. In the study group, the magnitude of SRT70 improvement was not correlated to the total number of AT hours. A large inter-patient variability was observed for speech-in-noise intelligibility measured once the AT program was completed and at re-test. The results suggest that serious game-based AT may improve speech-in-noise intelligibility in adult CI users. Potential sources of inter-patient variability are discussed. Serious gaming may be considered as a complementary training approach for improving CI outcomes in adults.
Differences in Vascular Density between Detached and Nondetached Areas in Eyes with Rhegmatogenous Retinal Detachment
Authors: Sato Mariko , Iwase Takeshi
Published: May 2022

We examined the vessel density (VD) of the deep capillary plexus (DCP) and choriocapillaris plexus (CCP) by optical coherence tomography (OCT) angiography in eyes with rhegmatogenous retinal detachment, which had similar amounts of detached and nondetached areas in the macula region, and then determined the morphology by OCT until 6 months after surgery. A total of 13 eyes of 13 patients whose average age was 55.8 ± 12.3 years and were successfully treated were enrolled in this study. Throughout the postoperative period, the VD of the DCP in the detached area decreased significantly compared to that in the nondetached area. Conversely, there was no significant difference in the VD of the CCP between the detached and the nondetached areas. The ratio of VD of both the DCP and CCP in the detached area to the in the nondetached area did not show significant changes during the follow-up period of 6 months. The ratio of VD of the DCP in the detached area to that in the nondetached area correlated significantly with the ratio of the external limiting membrane–ellipsoid zone (r = 0.57, p < 0.001) and ellipsoid zone–retinal pigment epithelium (r = 0.39, p < 0.001) thickness in the detached area to that in the nondetached area. A well-preserved DCP blood flow could result in the restoration of the outer retina.
Reliability and Time Efficiency of Digital vs. Analog Bite Registration Technique for the Manufacture of Full-Arch Fixed Implant Prostheses
Authors: Nuytens Philippe , D’haese Rani , Vandeweghe Stefan
Published: May 2022

Objective: Information about full-digital protocols for bite registration with intraoral scanners on multiple implants in the edentulous jaw is scarce. The purpose of this comparative in vivo study was to investigate the reliability and time efficiency of a novel full-digital bite registration technique for the manufacture of full-arch maxillary fixed implant prostheses. Material and methods: In ten patients, a full-arch maxillary fixed implant prosthesis was manufactured on multi-unit abutment level through an analog prosthetic workflow. The bite registration was performed with use of a screw-retained polymethyl methacrylate (PMMA) verification jig with detachable wax rim. To articulate the definitive edentulous maxillary implant cast in centric relation at the appropriate occlusal vertical dimension (OVD) to the mandibular antagonist cast, a type II articulator (Artex, Amann Girrbach) was used. Three to six months later, a full-digital bite registration was performed with use of dual-function scan bodies and bilateral connected bite pillars. The bite pillars screwed into the scan bodies were used to adjust and articulate the edentulous maxillary implant arch to the mandibular antagonist arch at the defined OVD. Treatment time for analog and digital bite registration technique was measured in each patient. The reliability of the digital bite registration technique was evaluated by 3D comparison of two sets of stereo lithographic (STL) files obtained from each patient. The three-dimensional deviation was defined along the X-, Y- and Z-axes (Geomagic Control X, 3D Systems Inc., Rock Hill, SC, USA). Results: The treatment time for digital bite registration using dual-function scan bodies and bite pillars was significantly shorter than analog bite registration with verification jig and wax rim (60.30%, SD 5.72%). Minor differences between the two techniques were observed with a linear deviation range of 1115 µm (SD 668 µm) overall, 46.2 µm (SD 731.3 µm) along the X-axis, −200.3 µm (SD 744.3 µm) along the Y-axis and 67.1 µm (SD 752.2 µm) along the Z-axis. Bilateral balanced contacts were registered in all patients during full-digital bite registration. Conclusions: The novel digital bite registration technique with dual-function scan bodies and bite pillars allows for a full-digital workflow for full-arch implant supported restorations. The digital bite workflow was 60% faster, and the overall deviation was around 1 mm, which can be considered clinically acceptable.
Valorization of a Highly Organic Sediment: From Conventional Binders to a Geopolymer Approach
Authors: Hussan Ali , Levacher Daniel , Mezazigh Salim , Jardin Louis
Published: May 2022

The objective of this research is to investigate the possible reuse of dredged sediments from the port of Cherbourg, France, as an alternative material in road engineering and as a backfill material. These dredged sediments contain high percentages of organic matter (OM), and the presence of OM in the sediment, even in small amounts, can affect the engineering properties of sediments. This research was carried out in two series: the sediment was treated with traditional hydraulic binders (ordinary Portland cement (OPC), calcium sulfo-aluminate (CSA) cement, quarry sand (QS), lime, and a combination of them) in the first series, and with pozzolanic binders in the second series (ground-granulated blast-furnace slag (GGBS) and fly ash (FA)), along with the introduction of an activator. According to French legislation, these two pozzolanic binders (GGBS and FA) have no carbon footprint as they are industrial by-products, and therefore, the second series of this research is considered to be highly eco-friendly and economical. Sediment treated with hydraulic binders yielded a maximum value of unconfined compressive strength (UCS) of 1 MPa at 28 days. Out of eight formulations made using traditional binders, only one formulation barely met the French criteria to be used in the sub-base layer of roads. The development of geopolymer using alkali-activated GGBS and then the incorporation of 30% sediments yielded a UCS value above 2 MPa at 28, 60, 90, and 180 days. Furthermore, the addition of 5% lime and 3% granular calcium carbonate in the same mixture (geopolymer + 30% sediments) increased the UCS by up to 60% and 90%, respectively.
Non-Destructive and Destructive Testing to Analyse the Effects of Processing Parameters on the Tensile and Flexural Properties of FFF-Printed Graphene-Enhanced PLA
Authors: Butt Javaid , Bhaskar Raghunath , Mohaghegh Vahaj
Published: May 2022

The significance of non-destructive testing (NDT) methods cannot be overstated as they help to evaluate the properties of a material without damaging/fracturing it. However, their applicability is dependent on their ability to provide reliable correlation with destructive tests such as tensile and flexural. This correlation becomes more problematic when the material is not homogeneous, such is the case with parts manufactured using a popular additive manufacturing process termed as fused filament fabrication (FFF). This process also requires optimisation of its parameters to achieve desired results. Therefore, this study aims to investigate the effects of four different nozzle temperatures, print bed temperatures, and print speeds on FFF-printed Haydale’s Synergy Graphene Enhanced Super Tough PLA through three non-destructive (ultrasonic, hardness, strain) and two destructive (tensile, flexural) testing methods. Samples were manufactured using Anet® ET4 Pro 3D printer and evaluated as per British and International standards. Two non-destructive tests, i.e., ultrasonic and hardness have been associated with evaluating the tensile properties of the manufactured parts. These results were correlated with destructive tensile testing and showed good agreement. The NDT method of strain measurement showed a very good correlation with the destructive three-point flexural test and was able to provide a reliable evaluation of flexural properties as a function of all three processing parameters. The results presented in this work highlight the importance of NDT methods and how they can be used to evaluate different properties of a material.
Current Concepts for Cutting Metal-Based and Polymer-Based Composite Materials
Authors: Trzepieciński Tomasz , Najm Sherwan Mohammed , Lemu Hirpa G.
Published: May 2022

Due to the variety of properties of the composites produced, determining the choice of the appropriate cutting technique is demanding. Therefore, it is necessary to know the problems associated with cutting operations, i.e., mechanical cutting (blanking), plasma cutting plasma, water jet cutting, abrasive water jet cutting, laser cutting and electrical discharge machining (EDM). The criterion for choosing the right cutting technique for a specific application depends not only on the expected cutting speed and material thickness, but it is also related to the physico-mechanical properties of the material being processed. In other words, the large variety of composite properties necessitates an individual approach determining the possibility of cutting a composite material with a specific method. This paper presents the achievements gained over the last ten years in the field of non-conventional cutting of metal-based and polymer-based composite materials. The greatest attention is paid to the methods of electrical discharge machining and ultrasonic cutting. The methods of high-energy cutting and water jet cutting are also considered and discussed. Although it is well-known that plasma cutting is not widely used in cutting composites, the authors also took into account this type of cutting treatment. The volume of each chapter depends on the dissemination of a given metal-based and polymer-based composite material cutting technique. For each cutting technique, the paper presents the phenomena that have a direct impact on the quality of the resulting surface and on the formation of the most important defects encountered. Finally, the identified current knowledge gaps are discussed.
Altered TIMP-3 Levels in the Cerebrospinal Fluid and Plasma of Patients with Alzheimer’s Disease
Authors: Park Jung Hyun , Cho Sun-Jung , Jo Chulman , Park Moon Ho , Han Changsu , Kim Eun-Joo , Huh Gi Yeong , Koh Young Ho
Published: May 2022

Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a component of the extracellular environment and is suggested to play an indirect role in regulating Aβ production and the pathophysiology of Aβ deposition in brains. However, studies on the amount of TIMP-3 in bodily fluids of Alzheimer’s disease (AD) patients have not been conducted. Here, we investigated the relationship between fluid TIMP-3 levels and AD pathology. We first showed that the fluid levels of TIMP-3 were lower in AD dementia patients compared with in non-AD patients. ELISA results revealed that plasma levels of TIMP-3 in 65 patients with AD were significantly lower than those in 115 healthy control subjects and 71 mild cognitive impairment (MCI) subjects. Furthermore, we found that cerebrospinal fluid (CSF) level of TIMP-3 was decreased in AD compared with that in healthy control. These data suggest that fluid TIMP-3 levels negatively correlated with progress of cognitive decline. Collectively, our study suggests that alterations of fluid TIMP-3 levels might be associated with AD pathology.
Expression Analysis of Genes Involved in Transport Processes in Mice with MPTP-Induced Model of Parkinson’s Disease
Authors: Rudenok Margarita M. , Shadrina Maria I. , Filatova Elena V. , Rybolovlev Ivan N. , Nesterov Maxim S. , Abaimov Denis A. , Ageldinov Ruslan A. , Kolacheva Anna A. , Ugrumov Michael V. , Slominsky Petr A. , Alieva Anelya Kh.
Published: May 2022

Processes of intracellular and extracellular transport play one of the most important roles in the functioning of cells. Changes to transport mechanisms in a neuron can lead to the disruption of many cellular processes and even to cell death. It was shown that disruption of the processes of vesicular, axonal, and synaptic transport can lead to a number of diseases of the central nervous system, including Parkinson’s disease (PD). Here, we studied changes in the expression of genes whose protein products are involved in the transport processes (Snca, Drd2, Rab5a, Anxa2, and Nsf) in the brain tissues and peripheral blood of mice with MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced models of PD. We detected changes in the expressions of Drd2, Anxa2, and Nsf at the earliest modeling stages. Additionally, we have identified conspicuous changes in the expression level of Anxa2 in the striatum and substantia nigra of mice with MPTP-induced models of PD in its early stages. These data clearly suggest the involvement of protein products in these genes in the earliest stages of the pathogenesis of PD.
Differential Activation of pERK1/2 and c-Fos Following Injury to Different Regions of Primary Sensory Neuron
Authors: Miao Bei , Yao Hongyu , Chen Peng , Song Xue-Jun
Published: May 2022

Nerve injury causes hyperexcitability of the dorsal root ganglion (DRG) and spinal dorsal horn (DH) neurons, which results in neuropathic pain. We have previously demonstrated that partial dorsal rhizotomy (PDR) produced less severe pain-like behavior than chronic constriction injury (CCI) or chronic compression of DRG (CCD) and did not enhance DRG neuronal excitability. However, the mechanisms underlying such discrepancy remain unclear. This study was designed to compare the activation of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) in DRG and DH, and c-Fos in DH following treatments of CCI, CCD, and PDR. We confirmed that thermal hyperalgesia produced by PDR was less severe than that produced by CCI or CCD. We showed that pERK1/2 in DRG and DH was greatly activated by CCI or CCD, whereas PDR produced only transient and mild pERK1/2 activation. CCI, CCD, and PDR induced robust c-Fos expression in DH; nevertheless, c-Fos+ neurons following PDR were much fewer than that following CCI or CCD. Blocking retrograde axonal transport by colchicine proximal to the CCI injury site diminished thermal hyperalgesia and inhibited pERK1/2 and c-Fos activation. These findings demonstrate that less severe pain-like behavior produced by PDR than CCI or CCD attributes to less activation of pERK1/2 and c-Fos. Such neurochemical activation partially relies on retrograde axonal transport of certain “injury signals” from the peripheral injured site to DRG somata.
Versatile and Resistant Electroless Pore-Plated Pd-Membranes for H2-Separation: Morphology and Performance of Internal Layers in PSS Tubes
Authors: Martinez-Diaz David , Michienzi Valeria , Calles José Antonio , Sanz Raúl , Caravella Alessio , Alique David
Published: May 2022

Pd-membranes are interesting in multiple ultra-pure hydrogen production processes, although they can suffer inhibition by certain species or abrasion under fluidization conditions in membrane reactors, thus requiring additional protective layers to ensure long and stable operation. The ability to incorporate intermediate and palladium films with enough adherence on both external and internal surfaces of tubular porous supports becomes crucial to minimize their complexity and cost. This study addresses the incorporation of CeO2 and Pd films onto the internal side of PSS tubes for applications in which further protection could be required. The membranes so prepared, with a Pd-thickness around 12–15 μm, show an excellent mechanical resistance and similar performance to those prepared on the external surface. A good fit to Sieverts’ law with an H2-permeance of 4.571 × 10−3 mol m−2 s−1 Pa−0.5 at 400 °C, activation energy around 15.031 kJ mol−1, and complete ideal perm-selectivity was observed. The permeate fluxes reached in H2 mixtures with N2, He, or CO2 decreased with dilution and temperature due to the inherent concentration-polarization. The presence of CO in mixtures provoked a higher decrease because of a further inhibition effect. However, the original flux was completely recovered after feeding again with pure hydrogen, maintaining stable operation for at least 1000 h.
ICP-MS Determination of Antimicrobial Metals in Microcapsules
Authors: Rezić Iva , Škoc Maja Somogyi , Majdak Mislav , Jurić Slaven , Stracenski Katarina Sopko , Vlahoviček-Kahlina Kristina , Vinceković Marko
Published: May 2022

Silver (Ag) and zinc (Zn) are very powerful antimicrobial metals. Therefore, in this research, a high-throughput, sensitive, and rapid method was developed for the determination of Ag and Zn in microcapsules using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple microwave digestion of the microcapsules with 55.55% v/v HNO3 and 44.45% v/v H2O2. The method was applied to determine Ag and Zn in microcapsule samples of different sizes (120 and 450 μm) after their preparation with and without chitosan. Prepared microcapsules, after characterization, were bonded to a polymer carrier by sol-gel procedure and the materials were characterized by FTIR spectroscopy and high-resolution optical microscopy. Significant differences were found in Ag and Zn levels between microcapsules samples prepared with and without chitosan. The results have shown that samples with chitosan had up to 20% higher levels of Zn than Ag: 120 μm microcapsules contained 351.50 μg/g of Ag and 85.51 μg/g of Zn, respectively. In contrast, samples prepared without chitosan showed larger overall variability: In microcapsules with a diameter of 120 μm, the amounts of antimicrobial metals were 98.32 μg/g of Ag and 106.75 μg of Zn, respectively. Moreover, 450 μm microcapsules contained 190.98 μg/g of Ag and 121.35 μg/g of Zn. Those quantities are high enough for efficient antimicrobial activity of newly prepared microcapsules, enabling the application of microcapsules in different antimicrobial coatings.