bluuebunny/crossref_metadata_2025 huggingface dataset:
TOPOGRAFI SETRA TUNON DESA ADAT PEJATEN, KECAMATAN KEDIRI, KABUPTEN TABANAN, BALI
Authors: Suassira I Wayan , Budiadi I Made , Wibawa I Gede Sastra
Published: November 2023

Desa Adat Pejaten terletak di Desa Pejaten, Kecamatan Kediri Kabupaten Tabanan. Setra Tunon yaitu tempat pemakaman sementara bagi masyarakat Hindu untuk menunggu waktu yang tepat dalam melaksanakan upacara ngaben. Saat ini kondisi dinding penahan tanah yang ada telah mengalami kerusakan. Oleh sebab itu, untuk menciptakan rasa kenyamanan dan keamanan diupayakan untuk dilakukan pembuatan serta perbaikan dinding penahan tanah. Desa Adat Pejaten berencana untuk melaksanakan renovasi secara bertahap untuk mencegah keruntuhan tanah yang lebih parah. Pentahapan renovasi yang akan dilaksanakan di mulai dengan merenovasi/membangun ulang dinding penahan tanah di sisi utara kemudian dilanjutkan dengan pembangunan di sisi timur nya. Sebelum di lakukannya renovasi/pembangunan perlu dilakukan pemetaan topografi tanah untuk mendapatkan informasi mengenai kontur tanah atau tinggi rendah permukaan. Peta topografi adalah salah satu jenis peta yang menggambarkan bentuk relief permukaan bumi, meliputi tinggi rendahnya kawasan dengan gambaran garis-garis. Garis yang dimaksud adalah garis kontur, yaitu garis yang menghubungkan daerah dengan ketinggian yang sama. Dengan adanya garis tersebut, maka akan memudahkan pengguna peta memahami ketinggian suatu tempat sehingga dapat memperkirakan kecuraman atau kemiringan lereng. Metode pelaksanaan yang dilakukan pada pengabdian masyarakan ini adalah Melakukan Pengukuran untuk pemetaan counter tanah menggunakan Total Station (TS). Hasil topografi/pemetaan tanah akan digunakan untuk keperluan desain/perencanaan dinding penahan tanah.  dan menyususun rencana anggaran biaya (RAB) pada kegiatan pengabdian selanjutnya sehingga permasalahan yang dihadapi oleh mitra bisa terselesaikan dengan tuntas.
XIII. Leó öröksége az Európai Unió jogában
Authors: Simon Tamás
Published: November 2023

Az antikvitásban is megjelent szubszidiaritás (kisegítés) elvének gondolatát a katolikus egyház csiszolta és tökéletesítette. A szubszidiaritás elvének lényege alapvetően abban áll, hogy a döntéshozatal az egyénekhez a lehető legközelebbi szinten történjen, ezzel elkerülve a felesleges központosítást, és ösztönözve a hatékony decentralizált működést. Az alapelv mögött annak felismerése áll, hogy a magasabb kormányzati szintek nem mindig rendelkeznek megfelelő szintű rálátással vagy megértéssel a helyi valóságot illetően, ezért a döntéshozatalt a legalacsonyabb hatáskörrel rendelkező szinten érdemes meghozni, a legjobb minőségű kormányzás elérése érdekében.A szubszidiaritás elve ma az Európai Unió döntéshozatali rendszerének egyik alapelveként érvényesül. A tagállami alkotmánybíróságok és az Európai Unió Bírósága (EUB) között az elmúlt években feléledt alkotmányossági vitákban a szubszidiaritás elvéhez való visszatérésnek fontos szerep juthat. A tanulmány bemutatja a szubszidiaritás elvének fejődési ívét az Európai Unió döntéshozatalában, valamint kitér azokra az intézményekre és eljárásokra, amelyek az elv érvényesülését hivatottak garantálni. Megvizsgálva a szubszidiaritás elvének politikai és jogi kikényszeríthetőségét, a tanulmány levonja a konklúziót az elv érvényesülésének jelenéről, és megfogalmaz néhány javaslatot a jövőt illetően.
The staffing problem in the police during 1943–1944 and ways to resolve it
Authors: Grechenko V. A.
Published: November 2023

The state of police staffing during 1943-1944 is revealed, its peculiarities related to the course of the German-Soviet war are identified. The staffing problem in the police during the war was extremely acute. The mobilisation of police officers to the army, their deaths in combat, and evacuation to the east significantly worsened the staffing potential of this law enforcement body. The situation with recruitment in 1943–1944 changed somewhat compared to the first stage of the war. This process became more manageable and lost the main elements of chaos and situationalism that were characteristic of the initial period of the war. In the regions of Ukraine liberated from the Nazis, NKVD operational groups immediately began recruiting police officers. There were several sources of this formation: pensioners, women, demobilised soldiers and partisans, as well as officers seconded from other regions of the USSR. The educational level of newly recruited police officers was very low at the time. Thus, of those recruited to the state police, only 7.6 % had special training, 3.1 % had military education, and 10.6 % had a secondary education. A significant proportion of police officers had little work experience. For example, 45 % of the state police had less than 1 year of service, and almost 69 % of the departmental police had less than 1 year of service. By the end of 1944, the total percentage of police personnel reached 70.4%. The ethnic composition of the police was as follows: Ukrainians – 47.8%, Russians – 36.5 %, Jews – 15.4 %, and others – 0.3%. Due to the shortage of people, large deviations from the mandatory conditions and rules for staffing the police were allowed. People who did not meet the requirements for police personnel in terms of age, health, education and generally did not meet the requirements for police personnel were recruited. This resulted in numerous official misconducts and crimes. The qualitative shortage of staff led to a significant turnover of personnel. To overcome the difficulties, this police staff learnt the basics of police service on the job, combining service with training. In addition, police officers received the necessary knowledge at various seminars and advanced training courses.
Sistem Pakar Dalam Menentukan Minat Dan Bakat Siswa Menggunakan Metode Forward Chaining
Authors: Aulia Fifa Nur
Published: November 2023

Minat adalah perasaan suka atau tertarik terhadap sesuatu atau dengan suatu kegiatan tanpa harus diminta. Bakat juga merupakan keterampilan atau kemampuan dasar seseorang untuk dapat berlatih dalam waktu yang lebih singkat dari orang lain, tetapi hasilnya akan lebih baik lagi. Dengan kurangnya pemahaman akan minat dan bakat setiap siswa maka peneliti akan merancang suatu sistem yang dapat membantu permasalahan tersebut. Di dalam komponen sistem pakar terdapat komponen antarmuka pengguna yang merupakan metode yang dibuat untuk berkomunikasi, sehingga siswa dapat berkomunikasi atau berkonsultasi. Sistem pakar ini menggunakan metode forward chaining. Metode ini bekerja dengan cara sistem akan menerima fakta dari pengguna, yang kemudian akan menghasilkan sebuah kesimpulan dari fakta tersebut. Pada penelitian ini digunakan rumus forward chaining untuk menentukan minat dan bakat siswa. Sistem yang dibangun pada penelitian ini menggunakan bahasa pemrograman PHP dengan database MYSQL. Hasil dari sistem yang telah dirancang adalah mengetahui bakat dari setiap siswa.
ВПЛИВ СТАВЛЕННЯ ДО ГРОШЕЙ НА СПОЖИВЧУ ПОВЕДІНКУ МЕНЕДЖЕРІВ
Authors: Вартанова О.В.
Published: November 2023

Досліджено особистісні фактори споживчої поведінки менеджерів, як-от: економність, раціональність розподілу коштів, гнучкість у виборі товарів, купівля як отримання бажаного. Виявлено, що більшість менеджерів раціонально розподіляють кошти, є гнучкими у виборі товарів, здійснюють покупки з метою задоволення бажань, демонструють високий рівень орієнтації на думку інших. Встановлено, що на споживчу поведінку менеджерів істотно впливає ставлення до грошей та грошова поведінка, що підтверджують результати кореляційного аналізу. За результатами опитування менеджери демонструють середній рівень грошової поведінки, а саме, таких факторів, як: грошова одержимість, гроші як влада, економність, неадекватна поведінка з грошима.
ВПЛИВ СОЦІАЛЬНОГО ІНТЕЛЕКТУ НА ЛОЯЛЬНІСТЬ ТА ЗАЛУЧЕННІСТЬ СПІВРОБІТНИКІВ ЯК ЧИННИК ФОРМУВАННЯ КАДРОВОЇ БЕЗПЕКИ
Authors: Лядський І.К. , Дячков Д.В.
Published: November 2023

У статті проаналізовано вплив соціального інтелекту на залученість персоналу та його лояльність до компанії. Анкетування проведено на науково-педагогічному колективі Полтавського державного аграрного університету. Метою дослідження була перевірка можливого зв’язку соціального інтелекту з кадровою безпекою закладу вищої освіти, завдяки опитуванню колег-освітян. Науково-теоретичним підґрунтям для нашого дослідження стала наша попередня робота, яка продемонструвала зв’язок соціального інтелекту з патріотизмом людини. Лояльність працівника до своєї компанії цілковито узгоджується з цим критерієм, оскільки також засновується на психічній стабільності, порядності, відповідних морально-етичних орієнтирах. Наші дослідження виявили вірність цього припущення. Соціальний інтелект має достовірний позитивний зв’язок (p=0,01**) з лояльністю респондентів до своєї компанії. Аналогічним чином впливають на неї й компоненти соціального інтелекту, а саме обробка соціальної інформації, соціальні навички та соціальна обізнаність. Інший показник, який було досліджено – залученість персоналу. Він не показав достовірного зв’язку із соціальним інтелектом, що може бути пов’язано з переважним впливом на нього зовнішніх чинників, зокрема кадрової політики та лідерських якостей керівництва. Тож у подальших дослідженнях вважаємо за доречне поглибити вивчення саме зв’язку між соціальним інтелектом та лояльністю.
La Protección del Derecho Humano a la Nacionalidad en el Sistema Universal. Análisis Histórico-Evolutivo y Jurídico Actual
Authors: Fraga Marcos
Published: November 2023

El derecho a la nacionalidad constituye uno de los derechos humanos sujetos a protección en el sistema universal. Para el presente artículo se realizó un estudio histórico-evolutivo y jurídico actual del alcance y las distintas formas de tal protección, en función de evaluar la tutela de este derecho. Se utilizaron como métodos los análisis histórico y jurídico, a partir de la revisión bibliográfica de disposiciones normativas, informes, resoluciones y otros documentos relativos a la materia. Como principales resultados se obtuvo que la génesis del debate sobre la protección del derecho a la nacionalidad precisó la distinción entre personas apátridas y refugiadas. Además, que el sistema de protección universal reconoce un conjunto de obligaciones de los Estados para la prevención y reducción de la apatridia, pero el organismo internacional encargado de la cuestión se encuentra limitado a la promoción, asesoramiento y asistencia, por lo que resulta insuficiente como mecanismo de protección.
KAJIAN ETNOPEDAGOGI PADA KESENIAN BANGRENG DI KECAMATAN CILAWU KABUPATEN GARUT
Authors: TIANAWATI YANTI , Koswara Dedi , Sudaryat Yayat
Published: November 2023

Penelitian ini bertujuan untuk mengkaji nilai etnopedagogi yang terdapat pada kesenian Bangreng di Kecamatan Cilawu, Kabupaten Garut. Metode penelitian yang digunakan adalah metode kualitatif deskriptif. Data diperloleh dari hasil observasi, wawancara langsung ke lapangan dan dokumentasi. Instrumen yang digunakan dalam proses pengumpulan data adalah  kartu data untuk menginterpretsikan nilai-nilai etnopedagogik yang terdapat pada kesenian Bangreng.. Penelitian ini dilakukan di Kecamatan Cilawu, Kabupaten Garut, Provinsi Jawa Barat.  Hasil penelitian ini menujukkan bahwa etnopedagogi Sunda  muncul dalam kesenian Bangreng. Bagaimanapun, hal-hal yang ditemukan merupakan hasil interpretasi oleh peneliti. Etnopedagogi Sunda dalam seni bangreng diinterpretasikan berdasarkan rangkaiannya. Hal ini dikarenakan etnopedagogi Sunda dapat dilihat melalui aktivitas yang dimunculkan, ketika dijelaskan makna dari setiap rangkaiannya. Walaupun dalam seni bangreng mencakup tiga struktur yaitu rangkaian, alat, dan pelengkap, namun dari ketiga struktur tersebut rangkaian seni bangreng lebih tepat tersebut.

Penelitian ini bertujuan untuk mengkaji nilai etnopedagogi yang terdapat pada kesenian Bangreng di Kecamatan Cilawu, Kabupaten Garut. Metode penelitian yang digunakan adalah metode kualitatif deskriptif. Data diperloleh dari hasil observasi, wawancara langsung ke lapangan dan dokumentasi. Instrumen yang digunakan dalam proses pengumpulan data adalah  kartu data untuk menginterpretsikan nilai-nilai etnopedagogik yang terdapat pada kesenian Bangreng.. Penelitian ini dilakukan di Kecamatan Cilawu, Kabupaten Garut, Provinsi Jawa Barat.  Hasil penelitian ini menujukkan bahwa etnopedagogi Sunda  muncul dalam kesenian Bangreng. Bagaimanapun, hal-hal yang ditemukan merupakan hasil interpretasi oleh peneliti. Etnopedagogi Sunda dalam seni bangreng diinterpretasikan berdasarkan rangkaiannya. Hal ini dikarenakan etnopedagogi Sunda dapat dilihat melalui aktivitas yang dimunculkan, ketika dijelaskan makna dari setiap rangkaiannya. Walaupun dalam seni bangreng mencakup tiga struktur yaitu rangkaian, alat, dan pelengkap, namun dari ketiga struktur tersebut rangkaian seni bangreng lebih tepat tersebut.
ASXL1 mutations are associated with a response to alvocidib and 5-azacytidine combination in myelodysplastic neoplasms
Authors: Riabov Vladimir , Xu Qingyu , Schmitt Nanni , Streuer Alexander , Ge Guo , Bolanos Lyndsey , Wunderlich Mark , Jann Johann-Christoph , Wein Alina , Altrock Eva , Demmerle Marie , Mukherjee Sanjay , Ali Abdullah Mahmood , Rapp Felicitas , Nowak Verena , Weimer Nadine , Obländer Julia , Palme Iris , Göl Melda , Jawhar Ahmed , Darwich Ali , Wuchter Patrick , Weiss Christel , Raza Azra , Foulks Jason M. , Starczynowski Daniel T , Yang Feng-Chun , Metzgeroth Georgia , Steiner Laurenz , Jawhar Mohamad , Hofmann Wolf-Karsten , Nowak Daniel
Published: November 2023

Inhibitors of anti-apoptotic BCL-2 family proteins in combination with chemotherapy and hypomethylating agents (HMAs) are promising therapeutic approaches in acute myeloid leukemia (AML) and high-risk myelodysplastic syndromes (MDS). Alvocidib, a cyclin-dependent kinase 9 (CDK9) inhibitor and indirect transcriptional repressor of the anti-apoptotic factor MCL-1, has previously shown clinical activity in AML. Availability of biomarkers for response to the alvocidib + 5- AZA could also extend the rationale of this treatment concept to high-risk MDS. In this study, we performed a comprehensive in vitro assessment of alvocidib and 5-AZA effects in n=45 high-risk MDS patients. Our data revealed additive cytotoxic effects of the combination treatment. Mutational profiling of MDS samples identified ASXL1 mutations as predictors of response. Further, increased response rates were associated with higher gene-expression of the pro-apoptotic factor NOXA in ASXL1 mutated samples. The higher sensitivity of ASXL1 mutant cells to the combination treatment was confirmed in vivo in ASXL1Y588X transgenic mice. Overall, our study demonstrated augmented activity for the alvocidib + 5-AZA combination in higher-risk MDS and identified ASXL1 mutations as a biomarker of response for potential stratification studies.
The Relation Between Psychological Stress and Locus of Control Among University Students
Authors: Alkhazaleh Mohammad Salman , Alomery Moath Kalef
Published: November 2023

The aim of this study is to investigate the correlation between psychological stress and locus of control among students at A University, while taking various factors into account. To achieve this objective, two scales were utilized: the psychological stress scale, previously used in Derany’s (2003) research, and the Locus of Control scale, developed by Rotter and translated to Arabic and coded by Barhoom (1999). The study sample included 353 randomly selected students, and the data was analyzed using the distributive method of analysis. The findings indicated that the level of psychological stress among the students was moderate, with an average of 2.34. The mental area had the highest stress level, with a mean of 2.70, followed by the psychological area, with a mean of 2.32 and the physiological area, with a mean of 1.99. The majority of students exhibited an internal locus of control (59 percent), and there was a statistically significant difference between psychological stress and locus of control.
Cognitive Load Theory for ESL Students: Mixed Method to Employ Difficulty in Using Tenses While Writing
Authors: Shruthi S , Aravind B R
Published: November 2023

The present study aimed to determine learners’ problems in using English tenses. By utilizing the pre-test to have students compose their own paragraphs in 12 different tenses and through LearningApps.org, the researcher distributed the post-test self-created questions. The research employed a mixed research method, and the findings were presented. This study focused on 96 Bachelor of Technology students also learning English as a second language. The results show that past perfect tense, future perfect tense and simple past tenses are the most commonly problematic writing skills by the learners. These issues are linked to the difficulties of employing the appropriate aspect and tense in the appropriate context, interference from second languages, a lack of repetition in practice, and student’s level of language proficiency. In addition, it is recommended that teachers employ various methods that are appropriate to teaching tenses to help students develop their writing abilities.
The Relationship Between Stressful Life Events and Feelings of Hopelessness and Psychological Loneliness Among Al Balqa Applied University Students
Authors: Abu-albasal Nagham M.
Published: November 2023

Because of the complexity of modern life in all aspects, people can face different stressful life events, which also lead to different physiological disorders. The current study aimed to discover the relationship between stressful life events and feelings of hopelessness, and psychological loneliness. The study sample consisted of (294) students from Al-Balqa Applied University (females, 262; males, 32). The researchers used three tools: the stressful life events scale, the hopelessness scale and the psychological loneliness scale. The study results showed a positive correlation between stressful life events and feelings of hopelessness and psychological loneliness. Accordingly, future suggestions for research, policy and practices are presented.
Passion to Learn Well With Reflections of CGPA and Course Levels: Empirical Evidence
Authors: Vanichvatana Sonthya
Published: November 2023

Passion to learn well (PLW) is important. With limited time and resources, how instructors can pinpoint and prioritize students who need support and encouragement to learn well and achieve the desired class performance. Cumulative grade point average (CGPA) is one acceptable factor in evaluating students’ academic performance. This research studied the relationship between PLW and CGPA, and course levels. The study surveys students at an undergraduate Bangkok private university, business school, real estate major. The questionnaire surveys were conducted in November 2021, and employed quantitative methods and descriptive data analysis. The results show that the higher CGPA, the higher the levels of PLW. Students with a CGPA of 2.35 and below reflected the median level of PLW. Students with CGPA above 3.00 reflected PLW at high to the highest levels. Course levels also show a relationship with PLW. The higher the course levels – from junior to senior courses – the higher PLW. Senior courses had a higher proportion of PLW at high level than junior courses. The findings contribute by support with empirical evidence.
Exploring Self-Regulated Learning and Their Impact on Students’ Mathematical Communication Skills on the Topic of Number Patterns With the Blended Learning System
Authors: Ismail Rafki Nasuha , Yerizon  , Fauzan Ahmad
Published: November 2023

This research aims to describe students’ MCS on the topic of number patterns with a blended learning system and describe profile students’ self-regulation learning (SRL) in Junior High School SMPN 8 Padang. The descriptive and qualitative method was adopted. Data were collected using questionnaires, tests, and interviews. The first step in taking the subject is the administration of an online learning SRL questionnaire through a Google form, followed by the result analysis. The second step is the administration of offline tests to class VIII students, while the third is an online interview activity through WhatsApp based on the SRL. The results showed that 40% were able to express mathematical ideas orally, in writing, and through demonstration and description. Furthermore,40% were able to explain their understanding of written mathematical presentations orally. Only 20% could communicate digitally, through the use of computer technology. The results also showed that 47.5% of students have MCS, which is in the medium category. Approximately 69% agree students enrolled in an offline course adopted self-regulated learning.
Gamification: A Practitioner’s Case in Building Brands
Authors: Tian Vane-Ing , Wong Oi Yee Katherine , Hung Tak Hung Barry
Published: November 2023

Technological advancement and the increasing need for consumer engagement have brought a new trend in advertising and marketing – gamification. The stimulating and interactive nature of mobile games and possible game-style rewards allow marketers to promote their brands innovatively.

This paper aims to contribute to the research on gamification in marketing, focusing on how the in-game presence of branding affects brand recall, brand recognition, and product sales. The data are collected from a practitioner and a real mobile game developed specifically for a grocery retail chain with around 2,000 stores in Thailand. The paired t-test results show that advergame product placement significantly increases brand recognition and recall.
Hubungan Aktivitas Fisik dan Waktu Paparan Layar dengan Kualitas Hidup Terkait Kesehatan pada Mahasiswa Rumpun Ilmu Kesehatan
Authors: Kusuma R. Fitra Anggreni , Adliah Fadhia , Mustari Yery
Published: November 2023

Perubahan sistem pembelajaran dari offline menjadi online akibat dari pandemi Covid-19 dapat menyebabkan penurunan aktivitas fisik serta peningkatan waktu paparan layar diantara mahasiswa yang dikaitkan dengan rendahnya kualitas hidup terkait kesehatan, dimana hal tersebut mencerminkan kesehatan fisik dan mental individu atau kelompok dalam periode tertentu. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fisik dan waktu paparan layar dengan kualitas hidup terkait kesehatan pada mahasiswa rumpun ilmu kesehatan Universitas Hasanuddin. Penelitian ini merupakan penelitian komparatif dengan analitik kuantitatif yang menggunakan pendekatan cross-sectional study dengan jumlah sampel sebanyak 359 mahasiswa. Pengumpulan data dilakukan dengan cara pengambilan data primer menggunakan International Physical Activity Questionnaire (IPAQ) untuk aktivitas fisik, Screen-time Questionnaire untuk waktu paparan layar, dan Short Form-36 (SF-36) untuk kualitas hidup terkait kesehatan. Berdasarkan hasil analisis uji hubungan dengan Spermans Rho Test, menunjukkan tidak adanya hubungan antara aktivitas fisik dengan kualitas hidup terkait kesehatan diantara mahasiswa dengan nilai signifikansi antara aktivitas fisik dengan komponen kesehatan fisik dan mental masing-masing sebesar 0,288 dan 0,259 (p>0,05), dan terdapat hubungan yang berlawanan dengan tingkat keeratan yang sangat lemah antara waktu paparan layar dengan kualitas hidup terkait kesehatan dengan nilai signifikansi antara waktu paparan layar dengan komponen kesehatan fisik dan mental masing-masing sebesar 0,004 dan 0,001 (p<0,05).
Discussing Job Satisfaction and Organizational Perceptions: Voice of Practitioners
Authors: Ketaren Budi Sahputra , Perangin-angin Deliana Br , Br Ginting Emmi , Sihombing Hendrick A , Swanda Mide Parma , Baihaqi Arif Fahri , Yusriani Sri
Published: November 2023

To explore determinants of job satisfaction and factors influencing organizational perceptions using qualitative literature and focus group discussions (FGDs) with cross-departmental managers. From 55 articles on ScienceDirect and Google Scholar, 12 pivotal studies (2018-2023) were analysed. Topics ranged from cultural aspects in the Arab world to challenges of work-life balance in the digital age. The research unveils intertwined personal, organizational, and societal dynamics impacting job satisfaction and organizational perceptions. Highlighted is the need for strategies addressing these complexities to boost job satisfaction across sectors. This study uniquely merges academic perspectives with practitioners' voices, offering an enriched viewpoint bridging theory and real-world experiences, making it a valuable reference for both academic discourse and practical strategy development.
Impact Management of Islands in Indonesia by Foreign Parties on The Economic Conditions of Surrounding Communities
Authors: Kusumawati Indah , Widiasih
Published: November 2023

Based on the website www.skproperty.org where some news has been found that a small portion of the islands in Indonesia have been managed by foreign parties, this is proven by various foreign websites which state that some of the islands have been sold even though the Agency National Intelligence stated that it was not for sale but to attract foreign investors to want to invest in developments in the area. what is really being sold, to understand this, we are interested in researching the impact of managing the islands by foreigners, methodology research by Literatur Review, and research results it turns out that it is not for sale but is an investment value for the development of the island to attract investors to invest their capital for development and economic growth in the region, Even though in reality attracting investors does not make the lives of the people around the island better, it would be wise if the management of the island through collaboration between the local community, investors and local government can work together to manage the island for marine tourism and grow the community's micro and small business units.
Sharia Economic Perspective on Online Loans Among Millennials
Authors: Amira  , Sugiarti Dian
Published: November 2023

Online loans have become a significant phenomenon among millennials, especially in today's digital era. In the perspective of Islamic economics, online loans are an interesting topic for analysis. First, we will examine aspects of sharia compliance in online loan transactions. In Islamic economics, there are principles such as the prohibition of usury (interest) and gharar (uncertainty) that must be considered. In the context of online loans, it will be analyzed whether the loan mechanism and fees applied are in accordance with these principles. Second, we will look at the social and economic impact of online loans on millennials.  Many millennials are experiencing financial difficulties and are using online loans as a quick solution. However, unwise use of online loans can have negative impacts, such as being trapped in a debt cycle that is difficult to overcome. In an Islamic economic perspective, it will be evaluated whether online loans provide long-term benefits for millennials and society as a whole. Third, alternative options will be discussed in an Islamic economic perspective that can be a solution for millennials in managing their finances. Concepts such as profit-sharing financing, term savings (mudarabah), and risk-sharing financial systems (takaful) will be studied as alternatives that are more in line with Islamic economic principles.
Favipiravir and ivermectin show in vitro synergistic antiviral activity against SARS-CoV-2
Authors: Jitobaom Kunlakanya , Boonarkart Chompunuch , Manopwisedjaroen Suwimon , Punyadee Nuntaya , Borwornpinyo Suparerk , Thitithanyanont Arunee , Avirutnan Panisadee , Auewarakul Prasert
Published: November 2023

Despite the urgent need for effective antivirals against SARS-CoV-2 to mitigate the catastrophic impact of the COVID-19 pandemic, favipiravir and ivermectin are among the common repurposed drugs that have been provisionally used in some countries. There have been clinical trials with mixed results, and therefore, it is still inconclusive whether they are effective or should be dismissed. It is plausible that the lack of clear-cut clinical benefits was due to the finding of only marginal levels of in vivo antiviral activity. An obvious way to improve the activity of antivirals is to use them in synergistic combinations. The in vitro antiviral activity of the combinations of favipiravir, ivermectin, niclosamide, and chloroquine against SARS-CoV-2 was assessed in Vero E6 cells and the lung epithelial cell, Calu-3. Here we show that favipiravir and ivermectin had synergistic effects against SARS-CoV-2 in Vero E6 cells. In addition, we found that favipiravir had an additive effect with niclosamide, another repurposed anti-parasitic drug with anti-SARS-CoV-2 activity. However, the anti-SARS-CoV-2 activity of favipiravir was drastically reduced when evaluated in Calu-3 cells. This suggested that this cell type might not be able to metabolize favipiravir into its active form and that this deficiency in some cell types may affect the in vivo efficacy of this drug. Favipiravir and ivermectin show the best synergistic effect. This combination is being tested in a randomized controlled clinical trial (NCT05155527).
KPT6566 induces apoptotic cell death and suppresses the tumorigenicity of testicular germ cell tumors
Authors: Sun Ruijing , Lee Eun Joo , Lee Seonock , Kim Gamin , Kim Jungho
Published: November 2023

Testicular germ cell tumors (TGCTs) frequently affect adolescent and young adult males. Although TGCT is more responsive to cisplatin-based chemotherapy than other solid tumors, some patients are nonresponders, and following treatment, many patients continue to experience acute and long-term cytotoxic effects from cisplatin-based chemotherapy. Consequently, it is imperative to develop new therapeutic modalities for treatment-resistant TGCTs. Peptidyl-prolyl isomerase (Pin1) regulates the activity and stability of many cancer-associated target proteins. Prior findings suggest that Pin1 contributes to the pathogenesis of multiple human cancers. However, the specific function of Pin1 in TGCTs has not yet been elucidated. TGCT cell proliferation and viability were examined using cell cycle analysis and apoptosis assays following treatment with KPT6566, a potent, selective Pin1 inhibitor that covalently binds to the catalytic domain of Pin1. A xenograft mouse model was used to assess the effect of KPT6566 on tumor growth in vivo. KPT6566 effectively suppressed cell proliferation, colony formation, and ATP production in P19 and NCCIT cells. Further, KPT6566 induced apoptotic cell death by generating cellular reactive oxygen species and downregulating the embryonic transcription factors Oct-4 and Sox2. Finally, KPT6566 treatment significantly reduced tumor volume and mass in P19 cell xenografts. The Pin1 inhibitor KPT6566 has significant antiproliferative and antitumor effects in TGCT cells. These findings suggest that Pin1 inhibitors could be considered as a potential therapeutic approach for TGCTs.
Tele-simulation in nutrition and dietetics students, impact on learning and perception: a study pilot
Authors: Piñuñuri Raúl , Muñoz-Castillo Catalina , Herrera-Aliaga Eduardo , Rodríguez-Palleres Ximena , Pino-Astorga Carolina , Estrada Lisbell D.
Published: November 2023

Introduction

During the COVID-19 pandemic, tele-simulation emerged as an educational alternative, being useful for the development of Nutritional Care Process (NCP) skills. The importance of this study lies in knowing the impact of tele-simulation on perception and learning for the NCP. The aim of this research was to know the academic performance and the student’s perception of their ability to apply a dietary anamnesis.

Materials and methods

A quantitative, longitudinal, three-temporal study was conducted with a purposive sample of 25 Nutrition and Dietetics students during 2021. All students performed three tele-simulations with a standardized patient, in relation to the NCP.

Results

The mean response among the three tele-simulations developed on perception in tele-simulation with a standardized patient was 4.6 (Scale 1–5). The aspect with the best perception of the students was observed in relation to the feasibility and viability of the tele-simulation. The students improved in disciplinary aspects such as the investigation of the culinary technique, representativeness and quantification of the intake through the application of the NCP.

Conclusion

Tele-simulation with standardized patient is viable as a teaching-learning methodology for the application of the PNC. It is possible to demonstrate a higher performance in the nutritional interview.
Genome-wide identification of long non-coding RNAs and their potential functions in radish response to salt stress
Authors: Sun Xiaochuan , Tang Mingjia , Xu Liang , Luo Xiaobo , Shang Yutong , Duan Weike , Huang Zhinan , Jin Cong , Chen Guodong
Published: November 2023

Long non-coding RNAs (lncRNAs) are increasingly recognized as cis- and trans-acting regulators of protein-coding genes in plants, particularly in response to abiotic stressors. Among these stressors, high soil salinity poses a significant challenge to crop productivity. Radish (Raphanus sativus L.) is a prominent root vegetable crop that exhibits moderate susceptibility to salt stress, particularly during the seedling stage. Nevertheless, the precise regulatory mechanisms through which lncRNAs contribute to salt response in radish remain largely unexplored. In this study, we performed genome-wide identification of lncRNAs using strand-specific RNA sequencing on radish fleshy root samples subjected to varying time points of salinity treatment. A total of 7,709 novel lncRNAs were identified, with 363 of them displaying significant differential expression in response to salt application. Furthermore, through target gene prediction, 5,006 cis- and 5,983 trans-target genes were obtained for the differentially expressed lncRNAs. The predicted target genes of these salt-responsive lncRNAs exhibited strong associations with various plant defense mechanisms, including signal perception and transduction, transcription regulation, ion homeostasis, osmoregulation, reactive oxygen species scavenging, photosynthesis, phytohormone regulation, and kinase activity. Notably, this study represents the first comprehensive genome-wide analysis of salt-responsive lncRNAs in radish, to the best of our knowledge. These findings provide a basis for future functional analysis of lncRNAs implicated in the defense response of radish against high salinity, which will aid in further understanding the regulatory mechanisms underlying radish response to salt stress.
CXADR polymorphism rs6517774 modifies islet autoimmunity characteristics and exhibits sex disparity
Authors: Nygård Lucas , Valta Milla , Laine Antti-Pekka , Toppari Jorma , Knip Mikael , Veijola Riitta , Hyöty Heikki , Ilonen Jorma , Lempainen Johanna
Published: November 2023

Enteroviral infections have been linked to the development of islet autoimmunity (IA) and type 1 diabetes (T1D), and the coxsackie and adenovirus receptor (CXADR) is one of the ligands used by adenoviruses and enteroviruses for cell internalization. Two CXADR single nucleotide polymorphisms (SNPs), rs6517774 and rs2824404, were previously associated with an increased susceptibility to IA in the international TEDDY study (The Environmental Determinants of Diabetes in the Young). This study aimed to replicate the results by genotyping 2886 children enrolled in the Finnish Diabetes Prediction and Prevention study (DIPP). In our preliminary analysis of the SNPs’ allelic distributions, we could not find any association with IA susceptibility. However, a stratified analysis revealed a sex disparity, since the allelic distribution of rs6517774 was different when comparing autoantibody positive females with males; a difference not seen in healthy subjects. By using HLA risk groups and sex as covariates, a Cox regression survival analysis found that the rs6517774 (A/G) SNP was associated with a lower age at seroconversion in females (Female*rs6517774-AA; HR = 1.53, p = 0.002), while introducing a protective effect in males. Accordingly, we propose that rs6517774 alters IA characteristics by modifying the age at seroconversion in a sex-dependent manner. In light of this observation, rs6517774 now joins a limited set on SNPs found to introduce sex-dependent risk effects on the age at IA initiation.
Pyroptosis: shedding light on the mechanisms and links with cancers
Authors: You Hong-mei , Wang Ling , Meng Hong-wu , Huang Cheng , Fang Guo-ying , Li Jun
Published: November 2023

Pyroptosis, a novel form of programmed cell death (PCD) discovered after apoptosis and necrosis, is characterized by cell swelling, cytomembrane perforation and lysis, chromatin DNA fragmentation, and the release of intracellular proinflammatory contents, such as Interleukin (IL) 8, IL-1β, ATP, IL-1α, and high mobility group box 1 (HMGB1). Our understanding of pyroptosis has increased over time with an increase in research on the subject: gasdermin-mediated lytic PCD usually, but not always, requires cleavage by caspases. Moreover, new evidence suggests that pyroptosis induction in tumor cells results in a strong inflammatory response and significant cancer regression, which has stimulated great interest among scientists for its potential application in clinical cancer therapy. It’s worth noting that the side effects of chemotherapy and radiotherapy can be triggered by pyroptosis. Thus, the intelligent use of pyroptosis, the double-edged sword for tumors, will enable us to understand the genesis and development of cancers and provide potential methods to develop novel anticancer drugs based on pyroptosis. Hence, in this review, we systematically summarize the molecular mechanisms of pyroptosis and provide the latest available evidence supporting the antitumor properties of pyroptosis, and provide a summary of the various antitumor medicines targeting pyroptosis signaling pathways.
Sedimentary processes in the bottom boundary layer of a contourite drift in the northern South China Sea
Authors: Wu Longwei , Zhao Yulong , Liu Zhifei , Ma Pengfei , Zhang Yanwei
Published: November 2023

Ocean dynamic processes in the bottom boundary layer (BBL) are crucial for sedimentation, such as deposition and resuspension of marine sediments. In this study, we conducted in-situ tripod observations of the sediment ridge of a contourite drift in the northern South China Sea to understand the main dynamic processes affecting sedimentation on the contourite drifts. It was found that the diurnal tidal current was the strongest current at the study site, thus acting as the main dynamic affecting sedimentation processes. Periodic events of elevated suspended sediment concentration (SSC) were observed, some of which occurred only within 15 m above the seafloor and were termed near-bottom high SSC events, while others covered the entire range of the observed water column and were termed full-depth high SSC events. In-situ sediment resuspension at the sediment ridge is not an important factor affecting the formation of high SSC events. Rather, these high SSC events were mainly caused by lateral transport of sediments from the main body of the contourite drift by the northwestward diurnal tidal currents. The seafloor sediments at the main drift body are resuspened owing to the near-critical reflection of diurnal tidal currents on the slope topography of the drift. During periods when diurnal tidal currents were weak, locally generated internal waves could also induce burst-like full-depth high SSC events. This study highlights the diurnal tidal current as the main dynamic regulating the sedimentary processes of the contourite drifts in regions where the near-critical reflection prone to occur, implying the complexity of sediment dynamics of contourite drifts.
Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration
Authors: Xie Bowen , Yang Fengyuan , Chen Hongguang , Zhang Hongxing , Ma Hebin , Li Tianqi , Chen Zhiqiang , Li Jingyuan , Li Xiaojie , Du Junjie
Published: November 2023

Objective: Electrospun nanofibers exhibit potential as scaffolds for articular cartilage tissue regeneration. This study aimed to fabricate electrospun polycaprolactone (PCL)/silk fibroin (SF) composite nanofiber scaffolds and to explore performance of the scaffolds for articular chondrocyte regeneration.

Methods: By altering material composition and preparation methods, three types of nanofiber scaffolds were effectively fabricated, including randomly oriented PCL (RPCL) nanofiber scaffold, randomly oriented PCL/SF (RPCL/SF) nanofiber scaffold, and aligned PCL/SF (APCL/SF) nanofiber scaffold. Physiochemical analyses were performed to determine mechanical properties and surface hydrophilicity of the nanofiber scaffolds. In vitro studies were conducted to investigate performance of the scaffolds on articular chondrocyte proliferation, gene expression and glycosaminoglycan secretion. Cytoskeleton staining was used to observe the arrangement of chondrocytes along the direction of the fibers and their elongation along the fiber arrangement.

Results: The physicochemical analysis demonstrated that the APCL/SF nanofiber scaffold exhibited improved mechanical properties and surface hydrophilicity compared to the RPCL and RPCL/SF nanofiber scaffolds. Furthermore, the in vitro cell culture studies confirmed that the APCL/SF nanofibers could significantly promote articular chondrocyte proliferation, type II collagen (COL-II) gene expression, and glycosaminoglycan secretion compared to the RPCL and RPCL/SF nanofiber scaffolds. Additionally, cytoskeletal staining displayed that the APCL/SF nanofiber scaffold promoted the elongation of articular chondrocytes in the direction of parallel fiber alignment.

Conclusion: The APCL/SF nanofiber scaffold exhibited promising potential as a composite scaffold for articular cartilage regeneration.
Microbial dark matter sequences verification in amplicon sequencing and environmental metagenomics data
Authors: Barak Hana , Fuchs Naomi , Liddor-Naim Michal , Nir Irit , Sivan Alex , Kushmaro Ariel
Published: November 2023

Although microorganisms constitute the most diverse and abundant life form on Earth, in many environments, the vast majority of them remain uncultured. As it is based on information gleaned mainly from cultivated microorganisms, our current body of knowledge regarding microbial life is partial and does not reflect actual microbial diversity. That diversity is hidden in the uncultured microbial majority, termed by microbiologists as “microbial dark matter” (MDM), a term borrowed from astrophysics. Metagenomic sequencing analysis techniques (both 16S rRNA gene and shotgun sequencing) compare gene sequences to reference databases, each of which represents only a small fraction of the existing microorganisms. Unaligned sequences lead to groups of “unknown microorganisms” that are usually ignored and rarefied from diversity analysis. To address this knowledge gap, we analyzed the 16S rRNA gene sequences of microbial communities from four different environments—a living organism, a desert environment, a natural aquatic environment, and a membrane bioreactor for wastewater treatment. From those datasets, we chose representative sequences of potentially unknown bacteria for additional examination as “microbial dark matter sequences” (MDMS). Sequence existence was validated by specific amplification and re-sequencing. These sequences were screened against databases and aligned to the Genome Taxonomy Database to build a comprehensive phylogenetic tree for additional sequence classification, revealing potentially new candidate phyla and other lineages. These putative MDMS were also screened against metagenome-assembled genomes from the explored environments for additional validation and for taxonomic and metabolic characterizations. This study shows the immense importance of MDMS in environmental metataxonomic analyses of 16S rRNA gene sequences and provides a simple and readily available methodology for the examination of MDM hidden behind amplicon sequencing results.
Single-molecule tracking of PprI in D. radiodurans without interference of autoblinking
Authors: Zhai Fanfan , Hao Li , Chen Xiaomin , Jiang Ting , Guo Qianhong , Xie Liping , Ma Ying , Du Xiaobo , Zheng Zhiqin , Chen Kun , Fan Jun
Published: November 2023

Autoblinking is a widespread phenomenon and exhibits high level of intensity in some bacteria. In Deinococcus radiodurans (D. radiodurans), strong autoblinking was found to be indistinguishable from PAmCherry and greatly prevented single-molecule tracking of proteins of interest. Here we employed the bright photoswitchable fluorescent protein mMaple3 to label PprI, one essential DNA repair factor, and characterized systematically the fluorescence intensity and bleaching kinetics of both autoblinking and PprI-mMaple3 molecules within cells grown under three different conditions. Under minimal media, we can largely separate autoblinking from mMaple3 molecules and perform reliably single-molecule tracking of PprI in D. radiodurans, by means of applying signal-to-noise ratio and constraining the minimal length for linking the trajectories. We observed three states of PprI molecules, which bear different subcellular localizations and distinct functionalities. Our strategy provides a useful means to study the dynamics and distributions of proteins of interest in bacterial cells with high level of autoblinking.
Serum zinc deficiency is a potential risk factor for the occurrence of levodopa-induced dyskinesia in drug-naïve Parkinson’s disease
Authors: Kim Joung Eun , Lee Hyo Sang , Jang Wooyoung
Published: November 2023

Background

Since environmental factors, especially heavy metals, were highlighted in the pathogenesis of Parkinson’s disease (PD), there are many epidemiologic studies regarding heavy metals and PD risk. However, longitudinal studies regarding the impacts of heavy metals on motor and nonmotor symptoms of PD are scarce.

Methods

In the current study, we compared the serum levels of five heavy metals, such as zinc(Zn), copper(Cu), lead(Pb), mercury(Hg), and manganese(Mn), in 111 previously drug-naïve PD patients (n = 111) retrospectively. Among these 111 patients, 65 were PD patients without levodopa-induced dyskinesia (LID), while the other 46 had LID. We assembled clinical characteristics of PD and performed correlation analysis with heavy metal levels. At baseline, all subjects were examined with 18F-N-(3-fluoropropyl)-2β-carboxymethoxy-3β-(4-iodophenyl) nortropane positron emission tomography/computed tomography (FP-CIT PET/CT). We used Cox proportional hazards regression analysis for determining factors relevant to the time to LID development in PD subjects.

Results

Zn deficiency was significantly higher in the PD with LID group than in the PD without LID group (79.58 ± 12.28 versus 88.16 ± 15.15 μg/L). Lower serum Zn levels were significantly correlated with age of onset, levodopa equivalent daily dose (LEDD) at 3 months, and Korean version of the Mini-Mental State Examination (K-MMSE) scores (r = 0.16, p < 0.05, r = − 0.20, p < 0.01, r = 0.28, p < 0.01). Additionally, Zn deficiency was associated with a reduced time to LID development in the adjusted model (HR 0.978, 95% CI 0.956–0.999).

Conclusion

This study suggests that serum Zn deficiency might be a risk factor for LID in drug-naïve PD patients.