bluuebunny/crossref_metadata_2025 huggingface dataset:
Participatory Learning Method: Effectiveness in Improving Student Learning Outcomes
Authors: Saroinsong Djony Max , Dengah Jenny Ieke , Rondonuwu Anneke Tienneke
Published: March 2024

The appropriate learning methods are needed to allow students to learn actively, dare to participate, and interact with each other in learning. This study aims to determine the effectiveness of the participatory learning method in improving student learning outcomes in educational sociology and anthropology courses. This research is a type of quantitative research with pre-experimental research methods and uses the one-group pretest-posttest design as the research design. The subjects in this study were undergraduate students from the nonformal education study program who contracted educational sociology and anthropology courses in the even semester of the 2021/2022 academic year at the Universitas Negeri Manado, with a research sample consisting of 15 students. Data collection techniques using test techniques. The research instrument used was a test of learning outcomes in the form of a pretest and a posttest in the form of a description. The results showed that the average posttest (3.37) was higher than the pretest (1.51). In addition, the average N-gain (g) acquisition score calculation is 0.75, which is included in the high category; thus, applying the participatory learning method effectively improves student learning outcomes in educational sociology and anthropology courses.
Need Analysis Seguyub Lasem Dance Media in the Feminine Stigma of Dance Learning for Middle Shool Students
Authors: Ayu Andira Agustina , Lestari Wahyu , Hartono Hartono
Published: March 2024

Practical dance learning at school is often stigmatizes as feminine by junior high school students, especially male students. Seguyub Lasem Dance Media Video is the development of dance learning media that uses YouTube as a learning platform with the aim of increasing students' interest in learning, eliminating the stigma of femininity in the dance, and helping students practice the Profil Pelajar Pacasila character education. The aim of the Analysis of Media Needs for the Seguyub Lasem Dance Video in the Feminine Stigma of Dance Learning for Middle School Students research is to describe students' learning interest in learning dance and to describe the media needs analysis for the Seguyub Lasem Dance video. The data collection techniques used were observation, interviews and documentation. The conclusion of the research is that teachers and students need the Seguyub Lasem Dance video media to increase students' interest in learning, eliminate the stigma of femininity in the art of dance, and help students practice the Profil Pelajar Pancasila character education..

Keywords: Need analysis, dance learning, feminine stigma
Effectiveness of Equal-Age Counselling Services to Prevent Early Marriage Attitude of Class IX MTS students Riyadhus Sholihin Sunggal
Authors: Ramadhani Layla Suci , Hasibuan M Fauzi
Published: March 2024

The study aims to find out the effectiveness of peer counselling services to prevent attitudes towards early marriage of students of 9th grade MTS Riyadhus Sholihin Sunggal. The research method used is experimental. In this study the researchers used the Pre Experimental research model using One Group Pre Test – Post Test Design. As to the data collection techniques used are (1) document analysis, namely list of names, number of students of class IX MTS Riyadhus Sholihi Sunggal and photo documentation of the research. (2) The test method used in this study is the filling of angket. (3) Observation, from the results of the analysis of data of counselling services for prevention of early marriage attitudes in students using the statistical analysis technique t-test, obtained t_count as 5.542 while the size of the number on the table of value t for one-party test (one tail test) with a degree of significance of 0.05 (5%) at d.b N-1= 9 is 1.833. Therefore, the result of the data analysis of 5,542 is above the limit of rejection of the zero hypothesis which is large 1,833 (t_count > t_table), so this study is significant.
A Qualitative Analysis of Obstacles and Obstacles in Implementing the EDLINK Application at the Surakarta
Authors: Amin Lailla Hidayatul , Nashir Muhammad Ja'far , Abdullah Haidar , Aulia Nurul , Zaki Ahmad Najmuddin
Published: March 2024

This research aims to conduct a qualitative analysis of the obstacles and obstacles that arise in implementing the EDLINK application at the Mamba’ul ‘Ulum Islamic Institute, Surakarta. This research uses a qualitative method with a case study approach. Data was collected through in-depth interviews with key informants, namely lecturers, administrative staff and students involved in using the EDLINK application. Other techniques used are observation and document analysis. Data were analyzed using qualitative data analysis techniques. The collected data was analyzed through a categorization process to identify the obstacles and barriers experienced by lecturers and students in using the EDLINK application. Based on the research results, it can be concluded that there are several obstacles and obstacles in implementing the EDLINK application at the Mamba’ul ‘Ulum Islamic Institute, Surakarta. Factors that cause these obstacles include limited infrastructure, lack of training and socialization, changes in work culture, and resistance factors. It is hoped that the results of this research will provide a comprehensive picture of the challenges faced in integrating technology in the higher education academic system. The findings of this research can provide valuable insight for the Mamba’ul ‘Ulum Islamic Institute to increase the effectiveness of implementing the EDLINK application and overcome obstacles that arise in the process. In addition, it is hoped that this research can contribute to the literature regarding the integration of technology in higher education academic systems.
Analyzing Cross-Linguistic Influence in Second Language Acquisition : A Bibliometric Study Using Vosviewer
Authors: Ratnasari Ratnasari , Am St. Asriati. , Wulan Dian Tanri , Syahruni Syahruni
Published: March 2024

This study aimed to determine the bibliometric data analysis of Cross Linguistic Influence in Second Language Acquisition using VOSviewer. It obtained the data through a reference management application. It used the keywords "Cross-linguistic, linguistic influence, language learning, and Second Language Acquisition" to search for data. Search data was taken from 2019 to 2023. The results showed that research data for five years obtained 219 articles. The number of studies every year fluctuates with a tendency to decrease. Most of the research occurred in 2019, with as many as 68 articles, and in at least 2023, as many as 4. The results of this study explain how important it is to use bibliometric analysis to discover the phenomena that occur regarding Cross Linguistic Influence in Second Language Acquisition. VOSviewer has analyzed numerous articles concerning the topic area, which is expected to stimulate researchers' enthusiasm in further developing research on this topic and function as a point of reference for upcoming material investigations
Effectiveness of Interactive Learning Media Based on Articulate Storyline in Increasing the Ability to Understand the Concept of a Healthy Home in Children with Cerebral Palsy
Authors: Sari Septrika Puspita , Nurhastuti Nurhastuti , Iswari Mega , Ardisal Ardisal , Tsaputra Antoni
Published: March 2024

This research was motivated by problems that occurred in one cerebral palsy student who was not yet able to understand the concept of a healthy home in class VIII. To overcome this, researchers aim to improve the ability to recognize the concept of a healthy home in children with cerebral palsy by using articulate storyline based interactive learning media. This type of research is experimental research using a quantitative approach in the form of Single Subject Research (SSR) with A-B-A design. Variable X in this research is interactive learning media based on an articulate storyline, while variable Y in this research is the ability to recognize the concept of a healthy home. The data collection techniques used are observation, tests and documentation. The data collection tool in this research is a test instrument in the form of a checklist. Data analysis was carried out using visual analysis methods. The research results showed that there was an increase in the ability to recognize the concept of a healthy home in children with cerebral palsy by using articulate storyline-based interactive learning media. This is proven by condition A1, during 3 meetings the child obtained a score of 45.4%, 45.4%, 45.4%. Condition B during 7 meetings the child obtained a score of 51.5%, 63.6%, 66.6%, 87.6%, 100%, 100%, 100%. Then, in condition A2 for 3 meetings the child obtained a score of 96.9%, 96.6%, 96.6%. After analysis, articulate storyline based interactive learning media was proven to be able to increase the ability to recognize the concept of a healthy home in children with cerebral palsy.
The Analysis of Student’s Interest in Reading English Text Books at SMP Negeri 27 Manokwari
Authors: Arsai Alfons Napoleon , Narwadan Maria Putri , Arsai Almendita F. W
Published: March 2024

The objective of this scholarly investigation is to discern the extent of eighth-grade students' engrossment with English textbooks at SMPN 27 Manokwari. The researchers employed a sophisticated research design by implementing a combination of quantitative and qualitative methodologies. The quantitative approach facilitated the systematic tabulation of data into numerical formats, while the qualitative methodology served to articulate and expound upon data devoid of numerical parameters. The participants in this erudite exploration comprised 20 eighth-grade students enrolled at SMPN 27 Manokwari, with the data collection tools encompassing meticulously crafted questionnaires and insightful interviews. Through meticulous data analysis, the research findings unveiled a noteworthy elevation in the students' ardor for perusing English textbooks, categorizing their interest as notably elevated. The diligent endeavors undertaken by the students in assimilating the contents of English textbooks encompassed practices such as preliminary readings before scheduled lessons, dedicated perusal within the precincts of the library, in-depth examination within the confines of their homes, and the proactive measure of seeking elucidation from the instructor when faced with incomprehensible subject matter. This intricate exploration not only gauged the students' proclivity for English textbook engagement but also illuminated the multifaceted strategies employed by them to fortify their understanding of the material at hand.
Music Course Learning Program Analysis: Study at Indra Music School Bandung
Authors: Moeradi Vista Amabile , Sukmayadi Yudi
Published: March 2024

Terselenggaranya pendidikan formal tentu perlu adanya dukungan tambahan sebagai upaya peningkatan tumbuh-kembang anak, salah satunya dengan pendidikan non-formal pada lembaga kursus musik. Artikel ini bertujuan menganalisis program pembelajaran kursus musik yang diselenggarakan oleh Indra Music School Bandung. Pendekatan kualitatif digunakan dengan metode analisis deskriptif dimana data dikumpulkan melalui observasi dan wawancara, kemudian dianalisis melalui teknik reduksi, verifikasi, dan ditarik kesimpulannya. Hasil penelitian mengungkap terdapat dua kurikulum yang digunakan Indra Music School, yaitu kurikulum independen hasil rancangan dan pengembangan lembaga itu sendiri serta kurikulum ABRSM. Proses pembelajarannya tidak memanfaatkan teknologi, hanya menggunakan buku sebagai pedoman pembelajaran. Penelitian ini diharapkan dapat memberikan pemahaman komprehensif terkait penyelenggaraan kursus musik pada lembaga-lembaga lainnya.
Development of Science Learning E-Module with Online Media on Water Pollution Matter in Lake Tondano
Authors: Jeujanan Anggelina , Tumbel Ferny Margo , Dungus Ferdy
Published: March 2024

Water pollution in Lake Tondano is closely related to science subjects in junior high school on environmental pollution matter. This research aims to produce science learning e-modules on water pollution in Lake Tondano with online media that are feasible and effective. This type of development research refers to the Borg and Gall development model. Data collection techniques used interviews, questionnaires, and tests. The research instruments used interview sheets, product validation questionnaires, product practicality questionnaires, and learning outcomes tests. The results showed that: (1) the validity test by learning media experts obtained a score of 94%, included in the very valid category (no need for revision); (2) the validity test by material experts showed a score of 91% included in the very valid category (no need for revision); (3) limited trials in small groups showed a score of 88% included in the very practical category; and (4) the average score of student learning outcomes of 77% included in the effective category. Based on the research that has been conducted, the science e-module with online media on water pollution material in Lake Tondano is declared feasible and effective for students to use.
The Content Analysis of English Textbooks Based on Alan Cunningsworth’s Criteria: A Systematic Review
Authors: Aki Agustinus Nai , Maru Mister G. , Oroh Elisabeth Z.
Published: March 2024

This investigation endeavors to elucidate the merits and demerits inherent in English textbooks as delineated in a myriad of scholarly articles dedicated to the content analysis of pedagogical materials for English instruction. A plethora of English textbooks, disseminated by governmental or non-governmental publishers, underwent scrutiny through the prism of various theoretical frameworks, most notably Alan Cunningsworth's discerning criteria. Employing a systematic review, this research meticulously unveils the commendable aspects and deficiencies encapsulated within the fabric of English textbooks or coursebooks expounded upon in the surveyed articles. A compendium of fifteen articles, spanning the temporal expanse from 2015 to 2023, was subjected to rigorous analysis through the discerning lens of Alan Cunningsworth's evaluative criteria. The discerning scrutiny revealed that all the scrutinized English textbooks impeccably adhered to and fulfilled the exacting standards posited by Cunningsworth's criteria. However, interspersed within these articles lay instances where certain textbooks failed to meet the stringent criteria delineated by Alan Cunningsworth. This investigation aspires to transcend its immediate findings, serving as a beacon of reference for future researchers and English educators engaged in the analytical or evaluative scrutiny of English instructional materials
Values of Moral Education in the Qur'an Suroh Ali Imran Verse 134
Authors: Auni Laila , Nasution Nur Azizah , Siregar Alimuddin
Published: March 2024

The background to this research is the widespread decline in morals found in various circles, both with oneself and with others. This happens due to a lack of knowledge which becomes the source of reason and spiritual input, namely advice and others, even because of following one's own desires, so that morals are not controlled and become the main problems that arise among society, such as greed , difficulty controlling emotions and selfishness, making it difficult to create other good deeds. Starting from this, the author was interested in studying more deeply the values of moral education contained in the QS. Ali Imran verse 134. This aims to find out the moral values contained in the QS. Ali Imran verse 134, so that you can increase your knowledge and efforts in implementing the message and commands of Allah SWT. This research is library research whose data was obtained from two sources. The first primary source is QS. Ali Imran verse 134. The two secondary sources are in the form of commentary books, books, articles and others that support this research. The method used is the Maudhu'i method with qualitative analysis and then reprocessed using deductive, inductive and comparative methods. The results of the research show that: 1) give to each other under any circumstances. 2) restrain emotions. 3) forgive other people's mistakes. 4) do good to people who have wronged them and this is the pinnacle of the nobility of a servant's morals and devotion.
The Effect of PBL Model and Learning Motivation on the Mathematical Connection Ability of Class VIII MTs Darussalam Batumarta Students
Authors: Rosidah Nur , Hadi Sopiyan , Noviyanti Mery
Published: March 2024

Students in the eighth grade during the second half of the 2022–2023 school year were the focus of this research, which examines how the project-based learning (PBL) model and students' intrinsic motivation to learn affected their capacity to make mathematical connections. There was a quantitative approach to this research. Methods such as observation, interviews, documentation, surveys, and testing are used to gather data. This study used a quasi-experimental design. Because the individuals already existed before to the research, subject selection was not random. Using the total sampling approach, 66 students, representing the whole eighth grade, were selected as the population. Both tests and non-tests are used in the data collecting technique. Research consists of many stages, such as planning, execution, and data processing. Data analysis approaches involve quantitative data analysis and data quality assessment. Based on the results of research and discussion, it is known that 1) there are differences in the mathematical connection ability of students who obtain PBL model learning with students who obtain conventional model learning; 2) there is a difference in the increase in the mathematical connection ability of students who obtain PBL model learning with students who obtain conventional model learning; 3) there are differences in the mathematical connection ability of students who obtain PBL model learning with students who obtain conventional model learning on strong learning motivation; 4) there are differences in the mathematical connection ability of students who obtain PBL model learning with students who obtain conventional model learning on weak learning motivation; 5) there is an interaction between the use of learning methods and student learning motivation on the ability to solve thematic connection problems; and 6) there is a significant influence or interaction between the use of PBL models and the level of learning motivation on mathematical connection ability.
The Importance of Emotional Intelligence in Education: Preparing an Emotionally Resilient Generation
Authors: Anaktototy Karolis , Samsudin Samsudin , Sopyan Muhamad , Herawati Tri Mulia , Meriyati Meriyati
Published: March 2024

Education is an important foundation in shaping individuals to face the complex dynamics of life. However, the focus on academic achievement often ignores the emotional aspect. In this case, emotional intelligence (EQ) emerges as a key factor in preparing the younger generation to face life's challenges. This research explores the importance of emotional intelligence in education, with the aim of preparing an emotionally resilient generation. The research method used is a qualitative type with descriptive and comparative studies. This research collects data from various sources, including literature reviews and relevant documents. Qualitative and comparative analysis was carried out to understand the concept of emotional intelligence and its implementation in education.The research results show that the integration of emotional intelligence in the educational curriculum strengthens students' social skills and helps them cope better with stress. The role of parents and the school environment in supporting students' emotional development is also highlighted. Students with good emotional intelligence tend to achieve better academic results, emphasizing the positive impact of EQ development in education. Thus, this research provides insight into the importance of strengthening emotional aspects in learning. This not only improves academic performance, but also prepares students to face life's challenges with strong confidence and fortitude.
The Effect of Size Project Based Learning on Problem Solving Skills In Government Science Students
Authors: Pasaribu Daniel
Published: March 2024

The purpose of this study was to determine the effect of Size Problem Based Learning on problem solving ability in government science students. This study is a type of meta-analysis research. Data sources in this study come from 10 national and international journals published from 2020-2024.  The criteria for data eligibility are research derived from SINTA and Scopus indexed journals and proceedings, participants in this study of social science students, research related to the effect of size project based learning on problem solving ability in political science students, and the sample size must be large 20 students. Purposive sampling data collection techniques. Data analysis is calculating the effect size value with the help of Microsoft Excel 2020. The results of the study concluded that there was a significant influence of differentiation learning on the profile of Pancasila students in social science students in Indonesia with an average value of effect size 10 studies of 0.878 and Standard error of 0.342 with a high effect size category.
Module Development Based on Project Based Learning on the Material Types of Heavy Equipment
Authors: Gula Viktorius Fikran Cahaya , Telaumbanua Arisman , Zebua Yelisman , Harefa Envilwan Berkat , Telaumbanua Aprianus
Published: March 2024

This study concentrates on crafting a module geared towards project-based learning (PBL) centered on heavy equipment material types. Given the increasing demand for adept heavy equipment operators, it is crucial to employ effective educational strategies to impart pertinent knowledge to learners. Commencing with an exhaustive literature review encompassing PBL, heavy equipment operations, and commonly utilized materials in heavy equipment manufacturing, the research proposes a systematic methodology for module development. This approach incorporates pivotal PBL principles like practical problem-solving, collaboration, and inquiry-based learning. The module's design seamlessly blends theoretical underpinnings with hands-on applications, thereby furnishing learners with practical experiences and opportunities for honing critical thinking and skills. Evaluation of the module's effectiveness is conducted through both qualitative and quantitative means, encompassing pre and post-assessments, student feedback, and performance evaluations. The outcomes underscore the module's efficacy in augmenting learners' comprehension of heavy equipment material types and their application in real-world scenarios. This research contributes to the enhancement of educational methodologies in heavy equipment operations by furnishing a structured framework for deploying PBL modules tailored to specific learning objectives.
Improving the Skills of Making a Wedding Dowry Through a Project Based Learning Model for Deaf Children
Authors: Mahersa Rosy , Budi Setia , Iswari Mega , Taufan Johandri
Published: March 2024

Tujuan penelitian ini adalah untuk mengembangkan kemampuan pembuatan mahar pernikahan dengan pendekatan project based learning. Analisis data deskriptif kuantitatif digunakan bersamaan dengan pendekatan penelitian tindakan kelas (PTK) sebagai metodologi penelitian. Penelitian ini hanya terdapat satu siklus saja. Dua orang siswa tunarungu tingkat SMALB Kelas X SLB N 2 Pariaman dijadikan sebagai subjek penelitian. Prosedur tes, observasi, dan dokumentasi digunakan dalam pengumpulan data penelitian PTK. Berdasarkan hasil ujian pengetahuan siklus I, penelitian PTK menawarkan kepada siswa tuna rungu pendekatan pembelajaran berbasis proyek yang efisien untuk meningkatkan kemampuan mereka dalam membuat mahar pernikahan. Siswa telah mencapai hasil yang diharapkan. dimana muridnya
Effectiveness of the STEAM based SETS Learning Model to Increase Student's Scientific Literacy in Science Learning
Authors: Kadir Dalimawaty , Santosa Tomi Apra , Iswanto Iswanto , Marzuki Kartini , Guna Bucky Wibawa Karya , Amri Miftachul , Widodo Hadi , Suyahman Suyahman
Published: March 2024

The purpose of this study was to determine the effectiveness of STEAM-based SETS learning to improve students' science literacy in science learning.  This type of research is a meta-analysis of data collection through the analysis of 11 national and international journals published in 2021-2024. Data sampling techniques through purposive sampling techniques. The inclusion criteria in this study are research accessed through the google scholar database; ERIC, Sciencedirect and Wiley, research related to the STEAM-based SETS model to improve students' science literacy in science learning, Research must be indexed by SINTA, Scopus and WOS and research has complete data to calculate effect size. Data search keywords are SETS learning models, STEAM, Science literacy and Science learning. Data selection process through PRISMA method. Data analysis is quantitative analysis with the help of Microsoft Excel application. The results concluded that the STEM-based SETS learning model had a significant effect on students' science literacy skills with an average value of effect size (ES= 0.834) in the high effect size category. The findings provide important information that STEM-based STEM learning modes are effective for improving students' science literacy in science learning with N-gain = 0.41.
Public Relations in Improving the Quality of Education
Authors: Utami Melia Putri , Fikri Rahmat , Setiyadi Bradley
Published: March 2024

The aim of this research is to determine community relationships in improving the quality ofeducation. The methodology of this research is library research. Obtaining information or datafrom the library and using it as a basis for study is the goal of library research. The results ofthis research show that public relations is an organization's reputation management strategyto determine public views and inform the public about how good public relations are inimproving the quality of education. In line with recent educational advances, educationalpublic relations professionals working with communities consider and seek to improve thequality of educational activities. A pleasant school environment is always created so thatstudents can receive education according to their abilities, and improving teachers' skills or expertise in managing schools can help improve the quality of education and ensure that theteaching given to students can be well absorbed.
Blended Learning Method to Optimize English Language Learning in Non-English Language Education Departments at Muhammadiyah University of Enrekang
Authors: Rahman Musdalifah Ifah , Ismaya Ismaya
Published: March 2024

Limited time to direct learning in class often made learning English less effective, especially for classes with various levels of English proficiency. In a higher education climate that demands active learning, English teachers often find it difficult to help students whose English competency was below average to catch up with their peers who had good competence. In general, this compulsory course was only held for one semester. In fact, the target of learning English on campus was generally quite high, namely to make students had sufficient English proficiency so that they could write academic papers in English as an international language. To help overcome these obstacles, this study offers the use of the blended language learning method, which was a learning method that complements direct learning with distant learning using the internet. Blended language learning was able to complement the lack of direct time in class with distance learning using internet media. This research was the result of writer's experience in applying this method for one academic year along with the results obtained from the application of the blended language learning method in the teaching process. With the application of the blended language learning method, the writer found that language learning at the college level could be more effective, efficient, and more student-centered even though the author still faces some obstacles in its application.
Age-related changes after intracerebral hemorrhage: a comparative proteomics analysis of perihematomal tissue
Authors: Li Xinhui , Xiao Zhongsong , Li Peizheng , Yang Wensong , Shen Yiqing , Liu Fangyu , Xiong Xin , Wu Qingyuan , Wang Peng , Dang Ruozhi , Gui Siwen , Deng Lan , Manaenko Anatol , Xie Peng , Li Qi
Published: March 2024

The risk factors and causes of intracerebral hemorrhage (ICH) and the degree of functional recovery after ICH are distinct between young and elderly patients. The increasing incidence of ICH in young adults has become a concern; however, research on the molecules and pathways involved ICH in subjects of different ages is lacking. In this study, tandem mass tag (TMT)-based proteomics was utilized to examine the protein expression profiles of perihematomal tissue from young and aged mice 24 h after collagenase-induced ICH. Among the 5,129 quantified proteins, ICH induced 108 and 143 differentially expressed proteins (DEPs) in young and aged mice, respectively; specifically, there were 54 common DEPs, 54 unique DEPs in young mice and 89 unique DEPs in aged mice. In contrast, aging altered the expression of 58 proteins in the brain, resulting in 39 upregulated DEPs and 19 downregulated DEPs. Bioinformatics analysis indicated that ICH activated different proteins in complement pathways, coagulation cascades, the acute phase response, and the iron homeostasis signaling pathway in mice of both age groups. Protein–protein interaction (PPI) analysis and ingenuity pathway analysis (IPA) demonstrated that the unique DEPs in the young and aged mice were related to lipid metabolism and carbohydrate metabolism, respectively. Deeper paired-comparison analysis demonstrated that apolipoprotein M exhibited the most significant change in expression as a result of both aging and ICH. These results help illustrate age-related protein expression changes in the acute phase of ICH.
Reconstruction of the genome-scale metabolic network model of Sinorhizobium fredii CCBAU45436 for free-living and symbiotic states
Authors: Ye Anqiang , Shen Jian-Ning , Li Yong , Lian Xiang , Ma Bin-Guang , Guo Feng-Biao
Published: March 2024

Sinorhizobium fredii CCBAU45436 is an excellent rhizobium that plays an important role in agricultural production. However, there still needs more comprehensive understanding of the metabolic system of S. fredii CCBAU45436, which hinders its application in agriculture. Therefore, based on the first-generation metabolic model iCC541 we developed a new genome-scale metabolic model iAQY970, which contains 970 genes, 1,052 reactions, 942 metabolites and is scored 89% in the MEMOTE test. Cell growth phenotype predicted by iAQY970 is 81.7% consistent with the experimental data. The results of mapping the proteome data under free-living and symbiosis conditions to the model showed that the biomass production rate in the logarithmic phase was faster than that in the stable phase, and the nitrogen fixation efficiency of rhizobia parasitized in cultivated soybean was higher than that in wild-type soybean, which was consistent with the actual situation. In the symbiotic condition, there are 184 genes that would affect growth, of which 94 are essential; In the free-living condition, there are 143 genes that influence growth, of which 78 are essential. Among them, 86 of the 94 essential genes in the symbiotic condition were consistent with the prediction of iCC541, and 44 essential genes were confirmed by literature information; meanwhile, 30 genes were identified by DEG and 33 genes were identified by Geptop. In addition, we extracted four key nitrogen fixation modules from the model and predicted that sulfite reductase (EC 1.8.7.1) and nitrogenase (EC 1.18.6.1) as the target enzymes to enhance nitrogen fixation by MOMA, which provided a potential focus for strain optimization. Through the comprehensive metabolic model, we can better understand the metabolic capabilities of S. fredii CCBAU45436 and make full use of it in the future.
Fragility assessment for the rainfall-induced embankments on silty soils
Authors: Zhou Haizuo , Ma Fujian , Yu Xiaoxuan , Zheng Gang
Published: March 2024

The fragility curve expresses the probability that an asset exceeds some serviceability state for a given level of environmental perturbation or other loadings. It is an important component in the quantitative risk analysis and resilience evaluation of infrastructure exposed to natural hazards. Incidences of over-settlement of embankments are increasingly reported due to more intense and longer-duration rainfall events. This paper develops fragility curves for the rainfall-induced embankment settlement. For this purpose, an embankment incorporating enhanced seepage and displacement analysis within unsaturated soil conditions is modelled based on a reported case history. A Monte Carlo simulation is used for rainfall infiltration and embankment deformation analysis under various rainfall scenarios. Probability values are obtained to achieve three levels of damage states in terms of road embankment settlement. The parametric analysis produces the exceedance probability curves for various rainfall intensities, saturated permeabilities and embankment slope angles. This work offers an efficient tool for assessing fragility to rainfall-induced excessive settlement of embankments.
A review of long non-coding RNAs in ankylosing spondylitis: pathogenesis, clinical assessment, and therapeutic targets
Authors: Wang Hanji , Yang Chengxian , Li Ge , Wang Boning , Qi Longtao , Wang Yu
Published: March 2024

Ankylosing spondylitis (AS) is a chronic immune-mediated type of inflammatory arthritis characterized by inflammation, bone erosion, and stiffness of the spine and sacroiliac joints. Despite great efforts put into the investigation of the disease, the pathogenesis of AS remains unclear, posing challenges in identifying ideal targets for diagnosis and treatment. To enhance our understanding of AS, an increasing number of studies have been conducted. Some of these studies reveal that long non-coding RNAs (lncRNAs) play crucial roles in the etiology of AS. Some certain lncRNAs influence the development of AS by regulating inflammatory responses, autophagy, apoptosis, and adipogenesis, as well as the proliferation and differentiation of cells. Additionally, some lncRNAs demonstrate potential as biomarkers, aiding in monitoring disease progression and predicting prognosis. In this review, we summarize recent studies concerning lncRNAs in AS to elucidate the underlying mechanisms in which lncRNAs are involved and their potential values as biomarkers for disease assessment and druggable targets for therapy.
Overnight monitoring reveals the behavioral rhythms of a geriatric male elephant: an animal-centered case study of rest and stereotypy
Authors: McGuire Alena , Kienlen Michelle , Emory Rachel , LaDue Chase A.
Published: March 2024

Introduction

Monitoring overnight behavior is important in assessing the overall wellbeing of ex-situ elephant populations, with recumbent rest and stereotypy as key indicators of welfare. However, there have been few studies that address the overnight behavior of singly housed male elephants with a history of stereotypy. We conducted an opportunistic case study of the overnight behavior (i.e., rest and stereotypy) of a singly housed geriatric male Asian elephant at the Oklahoma City Zoo to identify his overnight behavioral rhythms in response to physiological (musth) and environmental changes (habitat access, automatic timed feeders) and guide management strategies.

Methods

Infrared cameras were utilized to conduct continuous focal-animal sampling of the elephant’s behavior between 20:00 and 08:00 in indoor and outdoor habitats. Sampling occurred from January 2023 to October 2023, with a total of 179 overnight observations.

Results

The elephant exhibited recumbent rest for 175.66 ± 6.80 (mean ± SE) minutes per night and was engaged in stereotypy for 175.88 ± 9.68 minutes per night. While there was no significant relationship between stereotypy and the sexual state of musth, musth was associated with reduced durations of recumbent rest. Access to both indoor and outdoor habitats was significantly related to decreased stereotypy compared to indoor-only treatments. Recumbent rest occurred most frequently between 02:00 and 05:00, and stereotypic behavior was most common between 06:00 and 08:00. Contrary to our expectations, automatic feeders did not significantly reduce stereotypy.

Discussion

The results of this study provide insight into the behavioral patterns of a geriatric male Asian elephant, demonstrating the importance of implementing an animal-centered approach to enhance animal wellbeing. As the zoo-housed Asian elephant population grows and continues to age, this case study emphasizes the importance of developing comprehensive welfare strategies for the elephants in our care.
Free play and pedagogical play: a multiple-case study of teachers’ views of play in Chinese early learning centers
Authors: Wang Xinxin , Ye Pingzhi , Qiao Tianqi
Published: March 2024

Objective

Grounded in theories of globalization, this qualitative case study aimed to explore the understanding of play among Chinese teachers in private, for-profit Western style early learning centers.

Methods

The study encompassed 16 Chinese teachers working in four Western-style early learning centers. Data were gathered through semi-structured interviews. Following separate thematic analyses of each case, a cross-case analysis was conducted to compare and contrast the emerging themes, elucidating both commonalities and distinctions across the four cases.

Results

The findings from all four cases revealed a categorization of play into two main types: “play in class’” and “play out of class.” “Play out of class” was characterized as unstructured, enjoyable, and creative, emphasizing child autonomy and spontaneous learning. In contrast, “play in class” pertained to play-based curricula that were thoughtfully designed to align with specific teaching goals and learning objectives. It was seen as a structured method for fostering learning, highlighting the developmental appropriateness of such approaches.

Conclusion:

These findings underscore the educators’ recognition of the significance of play; however, it also illustrates that their perceptions have been shaped by the prevailing emphasis on children’s achievements in Chinese society.
Triglyceride glucose index is associated with functional coronary artery stenosis in hypertensive patients
Authors: Wang Shuting , Shi Zhenzhou , Pan Hong , Yan Tiancai , Liu Ling , Xu Jiaheng , Wang Wei , Zhang Tong
Published: March 2024

Background

The triglyceride glucose (TyG) index is an effective method for determining insulin resistance (IR). Limited research has explored the connection between the TyG index and functionally significant stenosis in hypertensive patients. Furthermore, the connections between the TyG index, fat attenuation index (FAI) and atherosclerotic plaque characteristics are also worth exploring.

Methods

The study screened 1622 hypertensive participants without coronary artery disease history who underwent coronary computed tomography angiography. The TyG index was calculated as ln (fasting glucose [mg/dL] * fasting TG [mg/dL]/2). Adverse plaque characteristics (HRPCs), high-risk plaques (HRPs), FAI, and CT-derived fractional flow reserve (FFRCT) were analyzed and measured for all patients. Functionally significant stenosis causing ischemia is defined as FFRCT ≤ 0.80. Two patient groups were created based on the FFRCT: the FFRCT < 0.80 group and the FFRCT > 0.80 group. In hypertensive patients, the association between the TyG index and FFRCT was examined applying a logistic regression model.

Results

The TyG index was higher for people with FFRCT ≤ 0.80 contrast to those with FFRCT > 0.80. After controlling for additional confounding factors, the logistic regression model revealed a clear connection between the TyG index and FFRCT ≤ 0.80 (OR = 1.718, 95% CI 1.097–2.690, p = 0.018). The restricted cubic spline analysis displayed a nonlinear connection between the TyG index and FFRCT ≤ 0.80 (p for nonlinear = 0.001). The TyG index increased the fraction of individuals with HRPs and HRPCs, FAI raised, and FFRCT decreased (p < 0.05). The multivariate linear regression analysis illustrated a powerfulcorrelation between high TyG index levels and FAI, FFRCT, positive remodeling (PR), and low-attenuation plaque (LAPs) (standardized regression coefficients: 0.029 [p = 0.007], -0.051 [p < 0.001], 0.029 [p = 0.027], and 0.026 [p = 0.046], separately).

Conclusion

In hypertensive patients, the TyG index showed an excellent association with a risk of FFRCT ≤ 0.80. Additionally, the TyG index was also linked to FAI, FFRCT, PR, and LAPs.
Evaluating the safety and efficacy of cryopreserved ovarian tissue transplantation in leukemia patients with different bone marrow remission status using xenotransplantation
Authors: Li Yanqiu , Ruan Xiangyan , Gu Muqing , Du Juan , Jin Fengyu , Cheng Jiaojiao , Li Yanglu , Jiang Lingling , Wang Zecheng , Yang Yu , Zhang Mingzhen , Mueck Alfred O.
Published: March 2024

Background

Leukemia patients undergoing cryopreserved ovarian tissue transplantation (OTT) may carry a high risk of disease induction. Measurable residual disease (MRD) in bone marrow is linked to an elevated risk of relapse. It is controversial whether leukemia patients must be allowed to achieve measurable residual disease negative (MRD-negative) status instead of measurable residual disease positive (MRD-positive) status before ovarian tissue cryopreservation (OTC).

Objective

To explore the safety and efficacy of OTT in acute leukemia patients with different MRD status by using xenotransplantation.

Method

Cryopreserved ovarian tissue from 19 leukemia patients was thawed and xenotransplanted to ovariectomized BALB/C nude mice (n=36). The mice were divided into 2 groups based on the patient’s MRD status before OTC: MRD-negative group (n=18) and MRD-positive group (n=18), additionally, a control group consisted of ovariectomized mice (n=9). Body weight was measured weekly and mortality, emaciation, and other abnormalities were recorded. Twenty-six weeks post-surgery, livers, spleens, uteruses, and ovarian grafts were removed for macroscopic and histological examinations to evaluate the efficacy of xenotransplantation and assess malignant cell contamination in mice.

Results

Follicle growth was visible in the ovarian grafts of the MRD-negative and MRD-positive groups. Compared with the ovariectomized group, a significant decrease in body weight (p<0.01) was noted, the uterine volume was notably larger, estradiol (E2) levels were significantly higher (p<0.01), and follicle-stimulating hormone (FSH) levels were significantly lower (p<0.001) in the other two groups. Mice in the MRD-positive group showed a significantly higher incidence of death (p<0.001) and emaciation (p<0.01), compared to the MRD-negative group. Histological observation revealed the presence of malignant cells in the grafts, livers, and spleens of 3 mice in the MRD-positive group. No abnormalities were observed in the mice from the MRD-negative group in both macroscopic and histological observations except one mouse was sacrificed for ascites unrelated to leukemia relapse.

Conclusion

For leukemia patients having ovarian tissue preserved in the first and only centralized human ovarian tissue cryobank in China, immunodeficient mice xenotransplantation can be a method to evaluate the safety and efficacy of OTT; the risk of malignant cell reimplantation due to OTT is higher in leukemia patients with MRD-positive status than those with MRD-negative status before OTC.
Harmonic suppression control strategy of distribution networks based on the distributed power flow controller
Authors: Zhu Chengzhi , Qiu Peng
Published: March 2024

New energy and multiple loads, such as distributed wind–solar storage and charging, are connected to the distribution network through power electronic converters, which increases the harmonic content in the distribution network and causes operational safety risks. In this paper, the mathematical model of harmonic current and harmonic power content in a distribution network is constructed by an equivalent circuit and vector diagram. The distributed power flow controller is proposed for harmonic control of the distribution network. The mathematical expressions of the harmonic current and harmonic power based on a distributed power flow controller are constructed, and the control strategy of the distributed power flow controller for harmonic control is proposed. The simulation results show that the proposed method can effectively suppress the fifth and seventh harmonics of the distribution network.
Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line
Authors: Huang Xuewei , Liu Weiye
Published: March 2024

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most globally devastating viruses threatening the swine industry worldwide. Substantial advancements have been achieved in recent years towards comprehending the pathogenesis of PRRSV infection and the host response, involving both innate and adaptive immune responses. Not only a multitude of host proteins actively participate in intricate interactions with viral proteins, but microRNAs (miRNAs) also play a pivotal role in the host response to PRRSV infection. If a PRRSV–host interaction at the protein level is conceptualized as the front line of the battle between pathogens and host cells, then their fight at the RNA level resembles the hidden front line. miRNAs are endogenous small non-coding RNAs of approximately 20–25 nucleotides (nt) that primarily regulate the degradation or translation inhibition of target genes by binding to the 3’-untranslated regions (UTRs). Insights into the roles played by viral proteins and miRNAs in the host response can enhance our comprehensive understanding of the pathogenesis of PRRSV infection. The intricate interplay between viral proteins and cellular targets during PRRSV infection has been extensively explored. This review predominantly centers on the contemporary understanding of the host response to PRRSV infection at the RNA level, in particular, focusing on the twenty-six miRNAs that affect viral replication and the innate immune response.