bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibiting Factors to the Implementation of Preferential Procurement Policy in the South African Construction Industry
Authors: Tau Lebogang Joseph , Ogunbayo Babatunde Fatai , Aigbavboa Clinton Ohis
Published: August 2024

The South African preferential procurement policy emerged from the demand for transparency, fair competition, value-for-money, standardised and benchmark pricing, and regulation of public procurement arrangements in the construction industry. The policy aims to address historical inequalities, support economic growth, and foster sustainable development. The effectiveness of the preferential procurement policy in South Africa is affected by the inhibiting factors of its implementation system. Given this, this study assesses the factors inhibiting preferential procurement policy implementation in the South African construction industry. This study reviewed the extant literature from online databases as a secondary data source to identify and understand the factors inhibiting procurement policy implementation. A quantitative research design using a closed-ended survey questionnaire surveyed 31 identified inhibiting factors affecting procurement policy implementation from the literature review. One hundred sixty-seven (167) questionnaires were retrieved from two hundred (200) distributed, representing an 83.5 per cent response rate, distributed through Google Forms to the respondents in Northwest Province, South Africa. The reliability of the data collection instrument was determined using Bartlett’s sphericity, Cronbach’s alpha, and Kaiser–Meyer–Olkin tests. The exploratory factor analysis findings established eight components from the 31 identified inhibiting factors affecting procurement policy implementation, which are the absence of due diligence in procurement screening, corruption and political interference in procurement systems, an ineffective regulatory framework supporting public procurement policy, discrepancies in award of contracts and the absence of dispute resolution, ambiguity in procurement selection criteria, poor enforcement mechanisms, cost discrepancies in advance payment, and excessive bureaucracy in procurement documentation. This study’s practical implications provide an understanding of establishing and prioritising procurement selection criteria, such as project requalification requirements, cost performance requirements, technology integration in the prequalification process, and contract change order requirements, which would improve procurement systems in the South African construction industry.
High-Performance UV–Visible Broad Spectral Phototransistors Based on CuPc/Cs3Bi2I9 Heterojunction
Authors: Wang Yurui , Jiang Benfu , Zhao Xijun , Chen Yuxing , Pan Xinxin , Yu Qing , Yao Bo
Published: August 2024

Phototransistors are three-terminal photodetectors that usually have a higher photocurrent gain than photodiodes due to the amplification of the gate electrode. In this work, a broad spectral phototransistor based on copper phthalocyanine (CuPc) and a Cs3Bi2I9 (CBI) heterojunction is fabricated by the full vacuum evaporation method. Due to the complementary UV–visible absorption of CuPc and CBI, the device exhibits superior performance under three different types of visible light illumination. The experimental results show that the structure of the organic/perovskite heterojunction active layer has the characteristics of good compatibility and a simple process. Meanwhile, by utilizing the superior light-absorption characteristics of perovskite materials and the strong exciton dissociation efficiency of a hetero-type heterojunction interface, the CuPc/CBI-PT exhibits a higher photoresponsivity, photosensitivity, specific detection rate, and lower operating voltage than the CuPc reference device. The stability test shows that the CuPc/CBI-PT can still obtain a 0.73 A/W photoresponsivity under 660 nm light illumination after being stored in the air for 360 h without any packaging. This indicates that the organic/perovskite heterojunction PT may be a good choice for the preparation of high-performance photodetectors.
Effects of Substrates on the Performance of Pt Thin-Film Resistance Temperature Detectors
Authors: Liu Dingjia , Jiao Ruina , Sun Chunshui , Wang Yong
Published: August 2024

Pt thin-film resistance temperature detectors (RTDs) have been fabricated by magnetron sputtering on various substrates, including silica, polyimide (PI) and LaAlO3 (LAO) (100) single crystal. The influences of different substrates on the performance of Pt thin-film RTDs have been studied. It is revealed that the substrates exhibit a significant dependence on the temperature coefficient of resistance (TCR). Silica, PI and LAO substrates yield TCRs of 3.2 × 10−3, 2.7 × 10−3 and 3.4 × 10−3 /K, respectively. The Pt thin-film RTDs on LAO substrate exhibit a significantly larger TCR, compared to most of the other reported values. These devices also demonstrate a fast response time of 680 μs, which is shorter than that of many other reported RTDs. Furthermore, Pt thin-film RTDs on PI substrates could serve as flexible detectors, maintaining a consistent linear relationship between resistance and temperature even when bent.
Nasal Microbiome in Granulomatosis with Polyangiitis Compared to Chronic Rhinosinusitis
Authors: Brożek-Mądry Eliza , Burska Zofia , Życińska Katarzyna , Sierdziński Janusz
Published: August 2024

Rhinosinusitis in granulomatosis with polyangiitis (GPA) is categorised as a secondary, diffuse and inflammatory chronic rhinosinusitis (CRS). It is one of the conditions that impacts the nasal microbiota. This study aimed to compare the nasal microbiomes of patients with GPA, CRS and NSP. A total of 31 patients were included in the study (18 GPA, 6 CRS and 7 nasal septum perforation (NSP)). In all patients, SNOT 22, a nasal endoscopy (Lund–Kennedy scale) and a brush swab were performed. The metagenomic analysis was carried out based on the hypervariable V3-V4 region of the 16S rRNA gene. At the genus level, statistically significant differences were observed in two comparisons: the GPA/NSP and the GPA/CRS groups. In the GPA/NSP group, the differences were related to four genera (Actinomyces, Streptococcus, Methylobacterium-Methylorubrum, Paracoccus), while in the GPA/CRS group, they were related to six (Kocuria, Rothia, Cutibacterium, Streptococcus, Methylobacterium-Methylorubrum, Tepidimonas). Patients with GPA had lower diversity compared to CRS and NSP patients. There were no statistically significant differences found for the Staphylococcus family and Staphylococcus aureus between the three groups.
The Effect of Therapeutic Hypothermia on Ischemic Brain Injury in a Rat Model of Cardiac Arrest: An Assessment Using 18F-FDG PET
Authors: Kim Daehee , Lee Woon Jeong , Woo Seon Hee , Lee Hye Won , Kim Bom Sahn , Yoon Hai-Jeon
Published: August 2024

Purpose: Therapeutic hypothermia (TH) is widely acknowledged as one of the interventions for preventing hypoxic ischemic brain injury in comatose patients following cardiac arrest (CA). Despite its recognized efficacy, recent debates have questioned its effectiveness. This preclinical study evaluated the impact of TH on brain glucose metabolism, utilizing fluorine-18-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) in a rat model of CA. Methods: Asphyxia CA was induced in Sprague-Dawley rats using vecuronium. Brain PET images using 18F-FDG were obtained from 21 CA rats, who were randomized to receive either TH or no intervention. Of these, 9 rats in the TH group received hypothermia under general anesthesia and mechanical ventilation for eight hours, while the remaining 12 rats in the non-TH group were observed without intervention. We conducted regional and voxel-based analyses of standardized uptake values relative to the pons (SUVRpons) to compare the two groups. Results: Survival rates were identical in both the TH and non-TH groups (67%). There was no discernible difference in the SUVRpons across the brain cortical regions between the groups. However, in a subgroup analysis of the rats that did not survive (n = 7), those in the TH group (n = 3) displayed significantly higher SUVRpons values across most cortical regions compared to those in the non-TH group (n = 4), with statistical significance after false-discovery rate correction (p < 0.05). Conclusions: The enhancement in SUVRpons due to TH intervention was only observed in the cortical regions of rats with severe encephalopathy that subsequently died. These findings suggest that the beneficial effects of TH on brain glucose metabolism in this asphyxia CA model may be confined to cases of severe ischemic encephalopathy.
Association of Sleep Patterns and Respiratory Disturbance Index with Physiological Parameters in Pediatric Patients with Self-Perceived Short Stature
Authors: Huang Jing-Yang , Liao Pei-Lun , Chang Hua-Pin , Su Pen-Hua
Published: August 2024

Objective: To investigate the relationships of sleep patterns and respiratory disturbance index (RDI) with key physiological parameters (height, body mass index (BMI), bone age (BA), and IGF-1 levels) in children aged 6 to 16 years with self-perceived short stature. Methods: For this cross-sectional study, conducted from October 2019 to November 2021, 238 children aged 6 to 16 years with self-perceived short stature were enrolled. The primary outcomes of sleep patterns and the RDI were non-invasively collected at home using the LARGAN Health AI-Tech Sleep Apnea and Sleep Quality Examination System, which operates based on polygraphy. Additionally, various physiological parameters, including height, BMI, bone age, and IGF-1 levels, were measured to assess their associations with sleep patterns and RDI. Results: Significant age-related reductions were observed in both the total and deep sleep durations. Children aged 6–9 years averaged 8.5 ± 1.0 h of total sleep, which decreased to 8.1 ± 1.1 h in ages 10–11 and further to 7.5 ± 0.9 h in ages 12–16 (p < 0.0001). Deep sleep followed a similar pattern, decreasing from 4.4 ± 1.1 h in the youngest group to 3.3 ± 1.0 h in the oldest (p < 0.0001). Notably, girls experienced significantly longer deep sleep than boys, averaging 4.0 ± 1.2 h compared to 3.6 ± 1.2 h (p = 0.0153). In a multivariable regression analysis, age (beta = 4.89, p < 0.0001) and RDI (beta = −0.54, p = 0.0022) were significantly associated with body height. Age and deep sleep duration (beta = −0.02, p = 0.0371) were significantly associated with BMI. Conclusions: The results demonstrate significant age-related decreases in the total and deep sleep duration among children with self-perceived short stature, along with a notable association between RDI and body height and an association between deep sleep duration and BMI. These findings suggest that sleep disturbances in pediatric endocrine patients are intricately linked with physiological growth parameters. The identified correlations underline the importance of monitoring sleep patterns in this demographic to better understand the impact of endocrine disorders on developmental health. Further research is needed to explore interventions that could alleviate these sleep disturbances, thereby potentially improving outcomes for the affected children.
An Adaptive High-Efficiency LED Backlight Driver
Authors: Xu Xinyu , Zhuo Qiyue , Jiang Chunhui , Zhou Zichao , Tang Junlong , Zou Wanghui
Published: August 2024

An adaptive high-efficiency light-emitting Diode (LED) backlight driver scheme has been proposed to address the issue of additional power loss caused by LED forward voltage variation. In this scheme, the peak current and the duty cycle of each LED channel are adjusted separately through an adaptive control algorithm to minimize the voltage drop on the linear current regulator (LCR) of each LED channel to reduce the excessive power loss in each LED channel and enhance the total power efficiency. A linear current regulator, suitable for adaptive control, is designed on a 0.18 μm 5V complementary metal-oxide-semiconductor (CMOS) process. Simulation results demonstrate that the linear current regulator can achieve a linearly adjustable channel current ranging from 0 to 48 mA with a current resolution of 0.2 mA. Across different process corners and temperatures, the maximum error for the full current range is less than 0.1%. The core area of chip layout is about 0.1 mm2. The complete driver prototype comprises the LCR chips, external power MOS transistors, digital module, and LED chains. The test results show that the power loss of the linear current regulator has been significantly reduced, and the power efficiency of each LED channel has been measured at around 98.1%.
Multifrequency Impedance Characterization for Radio Frequency Identification Chip
Authors: Scrofani Benoît , Deleruyelle Thibaut , Loussert Alain , Artigue Olivier
Published: August 2024

This article introduces an innovative methodology for measuring the impedance of RFID (Radio Frequency Identification) chips. The primary objective is to develop a technique that enables impedance measurement across a range of frequencies, centered around the critical operational frequency of RFID systems, specifically 13.56 MHz. Unlike conventional methods, which typically focus solely on the impedance at 13.56 MHz, this approach utilizes the reflection coefficient of the device under test (DUT) to measure impedance over a broader frequency spectrum. This spectrum encompasses the frequencies within the ISO14443 communication bandwidth. The excitation signal is carefully selected to closely mimic an actual RFID communication frame. The experimental results demonstrate the feasibility of this method by comparing the impedance measurements of passive component pairs against those obtained using a vector network analyzer (VNA). Subsequently, the technique is applied to an RFID chip, underscoring its practical applicability and accuracy.
A Practical Security Assessment Methodology for Power System Operations Considering Uncertainty
Authors: Nguyen Nhi Thi Ai , Le Dinh Duong , Ngo Van Duong , Pham Van Kien , Huynh Van Ky
Published: August 2024

Today, renewable energy sources (RESs) are increasingly being integrated into power systems. This means adding more sources of uncertainty to the power system. To deal with the uncertainty of input random variables (RVs) in power system calculation and analysis problems, probabilistic power flow (PPF) techniques have been introduced and proven to be effective. Currently, although there are many techniques proposed for solving the PPF problem, the Monte Carlo simulation (MCS) method is still considered as the method with the highest accuracy and its results are used as a reference for the evaluation of other methods. However, MCS often requires very high computational intensity, and this makes practical application difficult, especially with large-scale power systems. In the current paper, an advanced data clustering technique is proposed to process input RV data in order to the decrease computational burden of solving the PPF problem while upholding an acceptable level of accuracy. The proposed method can be effectively applied to solve practical problems in the operating time horizon of power systems. The developed approach is tested on the modified IEEE-300 bus system, indicating good performance in reducing computation time.
Unlocking Business Value: Integrating AI-Driven Decision-Making in Financial Reporting Systems
Authors: Artene Alin Emanuel , Domil Aura Emanuela , Ivascu Larisa
Published: August 2024

This research article investigates the synergies between artificial intelligence (AI), digital transformation (DT), and financial reporting systems within the business context. The central theme explores how organizations enhance their decision-making processes by integrating AI technologies into digital transformation initiatives, particularly in financial reporting. The focal point is comprehending how the synergy of these integrated systems can unlock substantial business value, instigate strategic innovation, and elevate overall financial analytics through the adoption of intelligent, data-driven decision-making methodologies. By harnessing advanced analytics, automation, and adaptive decision support capabilities, organizations navigate the complexities of a rapidly evolving business environment, in which neural networks emerge as a valuable tool for calibrating outcomes in the financial accounting environment, demonstrating effectiveness in processing complex financial data, identifying patterns, and making predictions, ushering in a new era of transformative possibilities. The introduction of a game theory payoff matrix in this AI decision-making tool adds a strategic framework for analyzing interactions among decision-makers, considering strategic choices and outcomes in a dynamic and competitive context.
Analysis and Prediction of Spring-Back in Cylindrical Helical Springs Using Analytical and Numerical Models
Authors: Zani Nicola , Solazzi Luigi
Published: August 2024

This research focuses on cylindrical helical springs with circular cross-sections made from carbon steel (SH 0.82% C) and stainless steel (AISI 302). The transformation from a linear bar to a circular spiral involves numerous factors such as material mechanical behavior, stress–strain relationships and residual stresses. This research investigates the spring-back phenomenon, which affects the final diameter of helical springs post-manufacture, using analytical, experimental and numerical methods. An analytical model, derived from the mechanical bending process, was proposed to predict spring-back, and its accuracy was validated against experimental data. This study also employed finite element simulations to analyze elastic recovery, confirming the analytical predictions. Results indicated that the spring-back ratio k could be expressed as an exponential function of the spring index C (the ratio between the final diameter of the spring D2 and the diameter of the wire DW), with a maximum error of 4.80% for stainless steel and 3.62% for carbon steel. This study’s findings provide valuable insights into optimizing the spring manufacturing process, enhancing the precision of spring diameter predictions, and potentially reducing production errors and material waste.
Functional Characterization of PagMYB148 in Salt Tolerance Response and Gene Expression Analysis under Abiotic Stress Conditions in Hybrid Poplar
Authors: Park Su Jin , Jang Hyun-A , Lee Hyoshin , Choi Hyunmo
Published: August 2024

MYB transcription factors containing an R2R3 DNA-binding domain are involved in the regulation of various physiological processes, such as responses to abiotic stresses, including salt stress. In poplar, various MYB transcription factors regulate stress responses. In this study, we aimed to identify the correlation between the transcription factor MYB148 and salt stress responses in the hybrid poplar (Populus alba × P. glandulosa). We induced a mutation in the MYB-binding domain of PagMYB148 using CRISPR/Cas9-mediated editing of the PagMYB148 gene, which induced a more suppressed expression of PagMYB148 than that in the wild-type. Although salt and drought treatments enhance the expression of PagMYB148, the pagmyb148-transgenic plants exhibited more sensitive phenotypes than the wild-type plants under salt stress. After exposure to salinity stress, the chlorophyll content was lower in pagmyb148-transgenic plants than in wild-type plants, whereas the mutation increased ion leakage from cells. Additionally, the expression of genes involved in the salt stress response was higher in pagmyb148-transgenic plants than in the wild-type. After salt treatment, pagmyb148-transgenic plants exhibited an increased level of H2O2 and reduced activity of antioxidant enzymes. In summary, the MYB148 transcription factor is involved in the regulation of salt stress resistance in hybrid poplar trees. This report contributes to providing a basis for further investigating the molecular mechanisms of the poplar PagMYB148 transcription factor under abiotic stress.
Artificial Intelligence of Things as New Paradigm in Aviation Health Monitoring Systems
Authors: Kabashkin Igor , Shoshin Leonid
Published: August 2024

The integration of artificial intelligence of things (AIoT) is transforming aviation health monitoring systems by combining extensive data collection with advanced analytical capabilities. This study proposes a framework that enhances predictive accuracy, operational efficiency, and safety while optimizing maintenance strategies and reducing costs. Utilizing a three-tiered cloud architecture, the AIoT system enables real-time data acquisition from sensors embedded in aircraft systems, followed by machine learning algorithms to analyze and interpret the data for proactive decision-making. This research examines the evolution from traditional to AIoT-enhanced monitoring, presenting a comprehensive architecture integrated with satellite communication and 6G technology. The mathematical models quantifying the benefits of increased diagnostic depth through AIoT, covering aspects such as predictive accuracy, cost savings, and safety improvements are introduced in this paper. The findings emphasize the strategic importance of investing in AIoT technologies to balance cost, safety, and efficiency in aviation maintenance and operations, marking a paradigm shift from traditional health monitoring to proactive health management in aviation.
Alport Syndrome: Clinical Utility of Early Genetic Diagnosis in Children
Authors: Christodoulaki Vasileia , Kosma Konstantina , Marinakis Nikolaos M. , Tilemis Faidon-Nikolaos , Stergiou Nikolaos , Kampouraki Afroditi , Kapogiannis Charalampos , Karava Vasiliki , Mitsioni Andromachi , Mila Maria , Kanaka-Gantenbein Christina , Makrythanasis Periklis , Tzetis Maria , Traeger-Synodinos Joanne
Published: August 2024

Alport syndrome (AS) is a hereditary glomerulopathy due to pathogenic variants in COL4A3, COL4A4, and COL4A5. Treatment with Renin–Angiotensin–Aldosterone System (RAAS) inhibitors can delay progression to end stage renal disease (ESRD). From 2018 until today, we performed Whole Exome Sequencing (WES) in 19 patients with AS phenotype with or without positive family history. Fourteen of these patients were children. Genetic testing was extended to family members at risk. All patients received a genetic diagnosis of AS: five X-linked AS (XLAS) males, five X-linked AS (XLAS) females, six autosomal dominant AS (ADAS), and one autosomal recessive AS (ARAS). After cascade screening four XLAS males and eight XLAS females, six ADAS and three ARAS heterozygotes were added to our initial results. Fifteen patients were eligible to start treatment with RAAS inhibitors after their diagnosis. All XLAS female patients, ARAS heterozygotes, and ADAS have been advised to be followed up, so that therapeutic intervention can begin in the presence of microalbuminuria. Genetic diagnosis of AS ensures early therapeutic intervention and appropriate follow up to delay progression to chronic kidney disease, especially in thet pediatric population.
Understanding the Basis of Schmitt’s Map of South Germany: Georeferencing the Sketches of Staržinsky and Sarret (Late 1790’s)
Authors: Kiss Eszter , Timár Gábor
Published: August 2024

Schmitt’s map was one of the outstanding survey products of the late 18th century, produced through Habsburg military mapping in the shadow of the Napoleonic Wars in the area of today’s southern Germany and some neighboring regions. The main geodetic basis for the map work was the series of surveys in Germany conducted by C.-F. Cassini de Thury in the 1760s. However, this was only a horizontal control for part of Schmitt’s map. The Cassini survey chains were linked in the 1790s by a complementary survey in the northern part of the map work: the Staržinsky-Sarret survey, which is the subject of this study. The authors have searched through the archive summary drafts of this survey. The georeferencing of the photographed sketches in the Cassini projection was feasible with surprisingly low error. By using the global SRTM elevation database, it was possible to identify the points/summits of the Staržinsky-Sarret survey between which visibility is possible. Thus, despite the fact that only one of the seven map sketches examined explicitly presents a triangulation structure, we present a possible triangulation pattern that could have been used to provide geodetic control in the northern part of the Schmitt map. The authors consider this survey as the basis for the assumption that georeferencing the Schmitt map in its own projection is possible in this area with relatively small residual errors.
Clodronate Reduces ATP-Containing Microvesicle Releasing Induced by Nociceptive Stimuli in Human Keratinocytes
Authors: Renò Filippo , De Andrea Marco , Raviola Stefano , Migliario Mario , Invernizzi Marco
Published: August 2024

Clodronate (Clod), a first-generation bisphosphonate, acts as a natural analgesic inhibiting vesicular storage of the nociception mediator ATP by vesicular nucleotide transporter (VNUT). Epidermal keratinocytes participate in cutaneous nociception, accumulating ATP within vesicles, which are released following different stimulations. Under stress conditions, keratinocytes produce microvesicles (MVs) by shedding from plasma membrane evagination. MV secretion has been identified as a novel and universal mode of intercellular communication between cells. The aim of this project was to evaluate if two nociceptive stimuli, Capsaicin and Potassium Hydroxide (KOH), could stimulate MV shedding from human keratinocytes, if these MVs could contain ATP, and if Clod could inhibit this phenomenon. In our cellular model, the HaCaT keratinocyte monolayer, both Capsaicin and KOH stimulated MV release after 3 h incubation, and the released MVs contained ATP. Moreover, Clod (5 µM) was able to reduce Caps-induced MV release and abolish the one KOH induced, while the Dansylcadaverine, an endocytosis inhibitor of Clod uptake, partially failed to block the bisphosphonate activity. Based on these new data and given the role of the activation of ATP release by keratinocytes as a vehicle for nociception and pain, the “old” bisphosphonate Clodronate could provide the pharmacological basis to develop new local analgesic drugs.
High Interleukin 21 Levels in Patients with Systemic Lupus Erythematosus: Association with Clinical Variables and rs2221903 Polymorphism
Authors: Espinoza-García Noemí , Salazar-Camarena Diana Celeste , Marín-Rosales Miguel , Reyes-Mata María Paulina , Ramírez-Dueñas María Guadalupe , Muñoz-Valle José Francisco , Borunda-Calderón Itzel María , González-Palacios Aarón , Palafox-Sánchez Claudia Azucena
Published: August 2024

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody production and diverse tissue and organ inflammatory affections. Interleukin 21 (IL-21) is implicated in B cell survival, proliferation, differentiation, class switching, and immunoglobulin production; therefore, it is considered a key cytokine in the pathogenesis of SLE. However, its association with disease activity and clinical phenotypes remains unclear. We aimed to evaluate the association of IL-21 levels with the disease activity and clinical phenotypes in patients with SLE. Also, we analyzed the IL21 polymorphisms associated with increased IL-21 levels. Methods: The IL-21 serum levels were determined using the enzyme-linked immunosorbent assay (ELISA) method. The rs2221903 and rs2055979 polymorphisms were assessed in 300 healthy controls (HCs) and 300 patients with SLE by the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique. The levels of IL-21 were monitored during follow-up visits in 59 patients with SLE. Results: The patients with SLE showed higher IL-21 levels compared to the HCs. The IL-21 levels did not correlate with Mex-SLEDAI and were not different in patients with inactive, mild–moderate, and severe disease. The IL-21 levels were increased in patients with hematological affection. The ROC curve analysis revealed that the IL-21 levels had good predictive power in discriminating among patients with SLE and HCs. In a follow-up analysis, the levels of IL-21 remained higher in the patients with SLE even when the patients were in remission. Also, the rs2221903 polymorphism was associated with increased IL-21 levels. Conclusions: This study highlights the importance of IL-21 as a key cytokine in SLE. IL-21 levels are higher in patients with SLE and remain increased regardless of disease activity. According to the ROC analysis, IL-21 is a potential biomarker of SLE. Further longitudinal studies are needed to explore the relationship between IL-21 and the clinical phenotypes of SLE.
Exploring the Frontiers of Ovarian Tissue Cryopreservation: A Review
Authors: Ramirez Tia , Pavone MaryEllen
Published: August 2024

Objective: This paper serves as an up-to-date narrative review of the most effective methods and outcomes of ovarian tissue cryopreservation (OTC) with new data comparing this method to oocyte and embryo cryopreservation as well as its utility in restoration of endocrine function. Background: Data on OTC are becoming more available as more patients are achieving cancer remission and choosing to use their cryopreserved tissue to conceive or restore endocrine function. With OTC only recently becoming a non-experimental method of fertility preservation, it is important to evaluate, compare, and optimize current practices to improve live birth outcomes. Methods: A literature search of meta-analyses, systematic reviews, case series, retrospective studies, and randomized control trials was performed using the PubMed database with multiple search terms. Discussion: Current practices and outcomes of OTC remain heterogeneous, though they are becoming more streamlined with the emerging data on successful live births. Multiple aspects of OTC have been studied to optimize protocols, particularly methods of cryopreserving, in vitro maturation, and transplantation. In vitro follicle maturation is a novel application with emerging data on methods and outcomes. OTC is a versatile method not only for fertility preservation but also for hormone restoration as well. With wider usage of OTC, ethical dilemmas will need to be addressed. Conclusions: OTC can be used as fertility preservation for a variety of patients. Recent studies suggest it may be comparable to embryo cryopreservation, but with growing data on live births, comparative studies should continue to be performed. In vitro follicle maturation (IVFM) is a promising application of ovarian tissue harvesting. Data are lacking on cost-effectiveness, patient satisfaction, and morbidity associated with OTC.
Safety and Efficacy of Combined Coronary and Peripheral Percutaneous Revascularization: A Proof-of-Concept Study
Authors: Canonico Mario Enrico , Verde Nicola , Avvedimento Marisa , Leone Attilio , Cutillo Maria , Simonetti Fiorenzo , Esposito Salvatore , Bardi Luca , Giugliano Giuseppe , Stabile Eugenio , Piccolo Raffaele , Esposito Giovanni
Published: August 2024

Background. Lower extremity peripheral artery disease (LEPAD) frequently coexists with coronary artery disease (CAD) in patients with multisite vascular disease (MVD). While percutaneous revascularization is well-established for both LEPAD and CAD, limited evidence exists for patients eligible for both procedures. Specifically, the feasibility of concomitant LEPAD and CAD percutaneous revascularization remains unknown. Objectives. To compare the efficacy and safety of concomitant coronary and lower extremity elective percutaneous revascularization. Methods. Between 2012 and 2021, we included 135 patients in an observational, retrospective single-center registry. The population was stratified into two groups: 45 patients (concomitant group) underwent simultaneous coronary and peripheral percutaneous interventions, and 90 patients (deferred group) underwent two separate procedures within one year. The primary efficacy endpoint was major adverse cardiovascular events (MACE) at one year, while the primary safety endpoint was in-hospital contrast-induced nephropathy (CIN). Results. Study groups were well-balanced in baseline characteristics. In terms of coronary features, the concomitant revascularization group more often underwent single-vessel percutaneous coronary intervention (PCI), while the deferred group had multivessel PCI with diffuse coronary disease. No differences were detected in the number of LEPAD lesions between groups. For the primary efficacy endpoint, the incidence of MACE at one year was 37.8% in the concomitant group vs. 34.4% in the deferred group (HR 1.20, 95% CI 0.64–2.10; p = 0.61). No significant differences were found in CIN occurrence between the concomitant and deferred groups (11.1% vs. 8.9%; OR 1.30; 95% CI 0.36–4.21; p = 0.68). Conclusions. Multisite vascular disease patients eligible for CAD and LEPAD percutaneous revascularization exhibited a high cardiovascular risk profile with diffuse multivessel coronary and lower extremity disease. Our study suggests the efficacy and safety of concomitant coronary and lower extremity percutaneous revascularization based on one-year MACE incidence and in-hospital CIN. However, dedicated studies are warranted to confirm the short- and long-term outcomes of the concomitant revascularization strategy.
The Derivation and External Validation of a Fibrosis Risk Model for Colorectal Tumours Undergoing Endoscopic Submucosal Dissection
Authors: Sferrazza Sandro , Maida Marcello , Calabrese Giulio , Facciorusso Antonio , Fuccio Lorenzo , Frazzoni Leonardo , Maselli Roberta , Repici Alessandro , Di Mitri Roberto , Santos-Antunes João
Published: August 2024

Background: Endoscopic submucosal dissection (ESD) is an advanced technique that can become more challenging in the presence of submucosal fibrosis. Predicting the grade of fibrosis is important in order to identify technically difficult ESD. Aims and Methods: Our study aimed to derive and validate a prediction model to determine the preoperative degree of submucosal fibrosis in colorectal tumours undergoing ESD. A predictive model was developed to derive the probability of an increasing submucosal fibrosis in the derivation cohort and then externally validated. Results: 309 patients (age: 68 ± 10.9 years) underwent colorectal ESD between January 2016 and June 2020. F0, F1, and F2 fibroses were reported in 196 (63.4%), 70 (22.6%), and 43 (13.9%) cases, respectively. R0 resection was found in 266 (87%) lesions. At multivariable analysis in the derivation cohort, lesion morphology (OR = 0.37 and CI = 0.14–0.97 for LST-NG vs. 0-Is; OR = 0.29 and CI = 0.1–0.87 for the LST mixed type vs. 0-Is; and OR = 0.32 and CI = 0.1–1.03 for LST-G vs. 0-Is) and increasing size (OR = 1.02 and CI = 1.01–1.04 for a 1 mm increase) were significantly associated with an increasing degree of fibrosis. The model had fair discriminating ability in the derivation group (AUROC = 0.61 and CI = 0.52–0.69 for F1–F2 vs. F0 fibroses; AUROC = 0.61 and CI = 0.45–0.77 for F2 vs. F0–F1 fibroses) and in the validation group (AUROC = 0.71 and CI = 0.59–0.83 for F1–F2 vs. F0 fibroses; AUROC = 0.65 and CI = 0.52–0.77 for F2 vs. F0–F1 fibroses). Conclusions: Our findings introduce a new tool for the stratification of ESD technical difficulty based on lesion size and morphological characteristics which could become crucial during the procedure’s planning process.
Evaluation of Anti-Thyroperoxidase (A-TPO) and Anti-Thyroglobulin (A-Tg) Antibodies in Women with Previous Hashimoto’s Thyroiditis during and after Pregnancy
Authors: Zaccarelli-Marino Maria Angela , Dsouki Nuha Ahmad , de Carvalho Rodrigo Pigozzi , Maciel Rui M. B.
Published: August 2024

Background/Objective: Autoimmune thyroid diseases (AITD) affect 2 to 5% of the general population. This study aimed to determine changes in activity of A-Tg and A-TPO antibodies before, during, and after pregnancy in women with previous AITD. Methods: This was a single-center study with a retrospective review of the medical records of 30 female patients aged 25–41 years who came to our endocrinology service in the city of Santo André, state of São Paulo, Brazil, to investigate thyroid diseases. The following data were reviewed: total triiodothyronine (totalT3), total thyroxine (totalT4), free thyroxine (FT4), thyroid-stimulating hormone (TSH), and anti-TSH receptor antibodies (anti-TSH receptor or anti-thyrotropin receptor antibodies (TRAb), anti-thyroid peroxidase (A-TPO), and anti-thyroglobulin (A-Tg)). These data were reviewed for 30 patients before and during the three trimesters of pregnancy and during the three months after pregnancy. Results: During gestation, we observed a progressive decrease in the blood values of A-TPO and A-Tg, which reached their lowest values in the third trimester of pregnancy, but after birth, they returned to values statistically equivalent to those before pregnancy. Analyzing the three trimesters and the post-pregnancy period, A-TPO increased 192% between the first trimester and postpartum (p = 0.009); it increased 627% between the second trimester and postpartum (p < 0.001); and it increased >1000% between the third trimester and postpartum (p < 0.001). There was no significant difference in the A-TPO values between the pre- and post-gestational periods (p = 1.00), between the first and second trimesters (p = 0.080), or between the second and third trimesters (p = 0.247). Conclusions: According to the results presented here, we observed changes in the activities of A-Tg and A-TPO antibodies during and after pregnancy in women with previous AITD. In women who intend to become pregnant, are pregnant, or have given birth within three months, it is essential to monitor A-TPO, A-Tg, and thyroid function as well as serum thyroid hormones and TSH to identify thyroid dysfunction in a timely manner and adjust the treatment strategy to avoid the deleterious effects of hypothyroidism on both mother and baby during and after pregnancy.
One-Month Duration Compared with Twelve-Month Duration of Dual Antiplatelet Therapy in Elective Angioplasty for Coronary Artery Disease: Bleeding and Ischaemic Outcomes
Authors: Corballis Natasha , Bhalraam U. , Merinopoulos Ioannis , Gunawardena Tharusha , Tsampasian Vasiliki , Wickramarachchi Upul , Eccleshall Simon , Vassiliou Vassilios S.
Published: August 2024

Background/Objectives: The need to determine the safest duration of dual antiplatelet therapy duration after elective angioplasty to reduce bleeding events without an adverse effect on major adverse cardiovascular events (MACE) remains a challenge. Methods: In this investigator-initiated, single-centre cohort study, we identified all patients who underwent PCI for de novo coronary disease for stable angina between January 2015 and November 2019. We compared 1-month and 12-month durations of dual antiplatelet therapy (DAPT) to determine if there was any difference in the primary outcome of major bleeding. The secondary outcome was a patient-oriented composite endpoint of all-cause mortality; any myocardial infarction, stroke, or revascularisation; and the individual components of this composite endpoint. Data were analysed using Cox regression models and cumulative hazard plots. Results: A total of 1025 patients were analysed, of which 340 received 1 month of DAPT and 685 received 12 months of DAPT. There was no difference in major bleeding between the two groups (2.6% vs. 2.5% respectively). On univariable cox regression analysis, no characteristics were predictors of major bleeding. A proportion of 99.7% of patients in the 1-month DAPT arm were treated with a DCB strategy, whilst 93% in the 12-month DAPT group were treated with a DES. There was no difference between the two groups with regards to the composite patient-oriented MACE (11% vs. 12%, respectively) or any individual component of this. These results were unchanged after propensity score matched analysis. Conclusions: A 1-month duration of DAPT, for which 99.7% of patients were treated with a DCB strategy, appears safe and effective when compared with a 12-month duration of DAPT with no difference in major bleeding or MACE.
Denosumab Re-Challenge and Long-Term Efficacy for Aneurysmal Bone Cyst of the Spine: Enhanced Treatment Algorithm
Authors: Evangelisti Gisberto , Altorfer Franziska C. S. , Falzetti Luigi , Palmerini Emanuela , Griffoni Cristiana , Ghermandi Riccardo , Boriani Stefano , Monetta Annalisa , Cesari Marilena , Ibrahim Toni , Gasbarrini Alessandro
Published: August 2024

Background/Objective: Surgical treatment of aneurysmal bone cysts (ABCs) can be challenging, especially in the spine. Non-surgical treatments such as with denosumab have shown promising results in different osteolytic pathologies. This retrospective observational study aimed to evaluate the long-term clinical and radiologic response of patients with ABCs of the mobile spine treated with denosumab and propose an updated treatment algorithm. Methods: Six patients with relapsed and symptomatic ABCs of the mobile spine were treated with denosumab (120 mg subcutaneously on days 1, 8, 15, 29, and every 4 weeks thereafter) between 2012 and 2023. Disease assessments were conducted using CT and MRI at 3, 6, 9, and 12 months post-treatment. Clinical data, including pain levels, symptoms, and adverse events, were documented from patients’ charts. Results: Patients underwent an initial phase of treatment with denosumab, receiving a mean of 22 administrations (range 13–42) over a median follow-up period of 41 months (range 15–98 months). Clinical improvement was observed in all patients after 4 weeks of treatment, and all patients demonstrated a radiological response after 12–24 weeks on denosumab. Three patients were progression-free after discontinuing denosumab following 13, 15, and 42 administrations, respectively. At the last follow-up, after 38, 43, and 98 months, these patients remained stable without relapse of the disease. Three patients had a relapse of disease after denosumab; two of them underwent denosumab re-challenge, while one patient received one mesenchymal stem cells (MSCs) injection. All patients showed clinical and radiological improvement and were resulted to be disease-free at the last follow-up. Conclusions: This study demonstrates the long-term efficacy and safety of denosumab in treating ABCs of the mobile spine, as well as the potential of re-challenge in managing recurrence. A treatment algorithm is proposed, positioning denosumab as a viable therapeutic option after other local treatments. Careful patient selection, monitoring, and further research are necessary to optimize denosumab use for ABCs.
Changing Knowledge, Principles, and Technology in Contemporary Clinical Audiological Practice: A Narrative Review
Authors: Brice Sophie , Zakis Justin , Almond Helen
Published: August 2024

The field of audiology as a collection of auditory science knowledge, research, and clinical methods, technologies, and practices has seen great changes. A deeper understanding of psychological, cognitive, and behavioural interactions has led to a growing range of variables of interest to measure and track in diagnostic and rehabilitative processes. Technology-led changes to clinical practices, including teleaudiology, have heralded a call to action in order to recognise the role and impact of autonomy and agency on clinical practice, engagement, and outcomes. Advances in and new information on loudness models, tinnitus, psychoacoustics, deep neural networks, machine learning, predictive and adaptive algorithms, and PREMs/PROMs have enabled innovations in technology to revolutionise clinical principles and practices for the following: (i) assessment, (ii) fitting and programming of hearing devices, and (iii) rehabilitation. This narrative review will consider how the rise of teleaudiology as a growing and increasingly fundamental element of contemporary adult audiological practice has affected the principles and practices of audiology based on a new era of knowledge and capability. What areas of knowledge have grown? How has new knowledge shifted the priorities in clinical audiology? What technological innovations have been combined with these to change clinical practices? Above all, where is hearing loss now consequently positioned in its journey as a field of health and medicine?
Neurosteroids in Glioma: A Novel Therapeutic Concept
Authors: Hogan Ava , Mut Melike
Published: August 2024

Glioma, a diverse group of brain and spinal cord tumors arising from glial cells, is characterized by varying degrees of malignancy, with some types exhibiting highly aggressive behavior, rapid proliferation, and invasive growth patterns, posing significant therapeutic challenges. This review delves into the complex interactions between glioma cells, neurotransmitters, and neurosteroids, emphasizing their potential as therapeutic targets. Key neurotransmitters, like glutamate and gamma-aminobutyric acid (GABA), play crucial roles in glioma growth, invasion, and treatment response. This review examines the involvement of neurosteroids in glioma biology and explores innovative therapeutic strategies targeting these systems. It encompasses the biosynthesis and mechanisms of neurosteroids, interactions between gliomas and neurotransmitters, the spatial distribution of neurosteroid synthesis in gliomas, the role of ion channels, hormonal influences, enzyme modulation, and the neuroimmune system in glioma progression. Additionally, it highlights the potential of neurosteroids to modulate these pathways for therapeutic benefit.
The Effect of Plastic Deformation on the Flattening of Friction Surfaces
Authors: Moshkovich Alexey , Lapsker Igor , Rapoport Lev S.
Published: August 2024

This work aims to demonstrate the discrepancy between the results achieved in the application of ball-on-flat devices. Meanwhile, the interaction between contact parameters and the morphology of friction surfaces will be considered. Flattening depends on the mechanical properties of contact materials and the variation in the deformed structure in surface layers. To evaluate the interaction between roughness parameters and contact pressure, wear, and morphology of the surfaces, a ball-on-disk rig was applied. The average groove sizes were measured on micro- and macroscales. The relation between groove sizes on micro- and macro scales is close to the same. The flattening sinusoidal ball-on-flat model was considered. The real friction and wear tests were used to analyze plastic deformation by accounting for dislocation gliding and the interaction between neighboring asperities. The relation of shear stresses to the interference of rough asperities was established. The effective plastic strain gradient was evaluated. The formation of a highly effective plastic strain gradient is associated with a high dislocation density. The effect of dislocation density on the hardening–softening of surface layers is considered.
Exploring Low-Grade Iron Ore Beneficiation Techniques: A Comprehensive Review
Authors: Bulayani Mompati , Raghupatruni Prasad , Mamvura Tirivaviri , Danha Gwiranai
Published: August 2024

The beneficiation of low-grade iron ores is a key research and development topic in the mineral processing industry. The gradual exhaustion of high-grade iron ore reserves, and rising consumer iron and steel demand globally necessitate efficient low-quality iron ore beneficiation to meet steelmaking quality requirements. This comprehensive review explores various beneficiation techniques for low-quality iron ore, focusing on conventional methods including comminution, froth flotation and gravity separation. This article discusses the principles, processes, and equipment used in these techniques and highlights recent advancements and research efforts in the field. This review also emphasizes the importance of effective beneficiation processes in enhancing economic viability, sustainable resource management, and environmental conservation. Furthermore, it presents a case study of iron ore deposits in Botswana, highlighting the potential economic growth and sustainable development that can be achieved by maximizing resource utilization through reductive roasting, followed by magnetic separation of iron ore using semi-bituminous coal as a reductant. Overall, this review provides valuable insights into low-grade iron ore beneficiation techniques and their significance in meeting the growing demand for high-quality iron and steel products.