bluuebunny/crossref_metadata_2025 huggingface dataset:
Public Attitudes Toward Psychiatric Hospitals: A Rural-Urban Comparative Public Survey in Odisha State, India
Authors: Jena Sunita , Zieger Aron , Böge Kerem , Salunkhe Gayatri , Schomerus Georg , Patel Kripalini , Padhi Bijaya Kumar , Ta Thi Minh Tam , Mungee Aditya , Hahn Eric
Published: October 2021

Background: Integration of psychiatric care with public health services and offering mental health care services to patients from lower socioeconomic status remains a global challenge. Scarcity of funds and professional workforce in psychiatric hospitals contribute to this situation. However, negative attitudes in the population are also a known impediment to patients seeking mental health services. This study aimed to assess the attitudes toward psychiatric hospitals among the urban and rural population in India.

Subjects and Methods: The study was carried out amongst the general population in Odisha, India. The total sample (n = 988) includes 496 respondents from an urban-setting, and 492 respondents from rural parts of the district. Participants were selected by using simple random-sampling from the Asian Institute of Public Health (AIPH) data base. A standardized seven-item questionnaire was adopted, with responses indicated on a 5-point Likert-scale. Interviews were fully structured and conducted face-to-face.

Results: Level of education (B = −0.192, ß = −0.320, p < 0.000) and urban-rural comparison (B = −0.272, ß = −0.189, p < 0.000) significantly influenced attitudes toward psychiatric hospitals. Gender, age, and religious beliefs did not show any significant effect on attitudes toward psychiatric hospitals. Individuals in rural areas and those with lower education levels showed more negative attitudes toward psychiatric hospitals.

Conclusion: Negative attitudes toward psychiatric hospitals from those living in rural areas as well as those with lesser education may be reflective of the lack of availability, accessibility, affordability, and credibility of such mental health services. The Mental Health Care Act in India is a progressive legislation which might improve the situation of the provided services and, consequently, reduce negative attitudes in the years to come.
Impact of Different Type and Frequency of Social Participation on Depressive Symptoms Among Older Chinese Adults: Is There a Gender Difference?
Authors: Xiao Shujuan , Lin Huang , Zhao Chongbang , Zheng Xiao , Shi Lei , Zhang Jiachi , Xue Benli , Chang Jinghui , Chen Jiangyun , Zhang Chichen
Published: October 2021

Objectives: Social participation may prevent depressive symptoms in older adults. But research to date ignores gender differences in the associations between social participation and depressive symptoms. The purpose of this study was to determine the effect of different type and frequency of social participation on depressive symptoms, as well as if there is a gender difference in these correlations among older Chinese adults.

Methods: Data was obtained from adults aged 60 years or above in the 2018 China Health and Retirement Longitudinal Survey, a nationally representative sample of older adults in China. Depressive symptoms were measured using CESD-10. Social participation included participation in social groups, hobby groups, sports groups, and community-related organizations. The independent relationships between each type of social participation and depressive symptoms were assessed using multiple linear regression models.

Results: A total of 6,287 older adults were included in this analysis, of whom 49.69% were women. Participating in social groups, sports groups, and community-related organizations with a frequency of one or more per week was all linked to better mental health. Furthermore, our findings suggest that the positive relationship between participation in social groups, hobby groups, and community-related organizations and depressive symptoms is more flexible for older men than for women.

Conclusions: Older individuals who participate in social participation at a high frequency may have better mental health. The findings provide novel insights into mental health from the standpoint of social participation in older adults. Gender differences in the associations between social participation and depressive symptoms need to be considered when formulating interventions to prevent depression.
Mitigating Burnout in an Oncological Unit: A Scoping Review
Authors: Alabi Rasheed Omobolaji , Hietanen Päivi , Elmusrati Mohammed , Youssef Omar , Almangush Alhadi , Mäkitie Antti A.
Published: October 2021

Objectives: The purpose of this study was to provide a scoping review on how to address and mitigate burnout in the profession of clinical oncology. Also, it examines how artificial intelligence (AI) can mitigate burnout in oncology.

Methods: We searched Ovid Medline, PubMed, Scopus, and Web of Science, for articles that examine how to address burnout in oncology.

Results: A total of 17 studies were found to examine how burnout in oncology can be mitigated. These interventions were either targeted at individuals (oncologists) or organizations where the oncologists work. The organizational interventions include educational (psychosocial and mindfulness-based course), art therapies and entertainment, team-based training, group meetings, motivational package and reward, effective leadership and policy change, and staff support. The individual interventions include equipping the oncologists with adequate training that include—communication skills, well-being and stress management, burnout education, financial independence, relaxation, self-efficacy, resilience, hobby adoption, and work-life balance for the oncologists. Similarly, AI is thought to be poised to offer the potential to mitigate burnout in oncology by enhancing the productivity and performance of the oncologists, reduce the workload and provide job satisfaction, and foster teamwork between the caregivers of patients with cancer.

Discussion: Burnout is common among oncologists and can be elicited from different types of situations encountered in the process of caring for patients with cancer. Therefore, for these interventions to achieve the touted benefits, combinatorial strategies that combine other interventions may be viable for mitigating burnout in oncology. With the potential of AI to mitigate burnout, it is important for healthcare providers to facilitate its use in daily clinical practices.

Conclusion: These combinatorial interventions can ensure job satisfaction, a supportive working environment, job retention for oncologists, and improved patient care. These interventions could be integrated systematically into routine cancer care for a positive impact on quality care, patient satisfaction, the overall success of the oncological ward, and the health organizations at large.
Food Insecurity and Sleep Disturbance Among 223,561 Adolescents: A Multi-Country Analysis of Cross-Sectional Surveys
Authors: Wang Qiuying
Published: October 2021

This study was to investigate the association between food insecurity (FI) and sleep disturbance among adolescents. Based on the cross-sectional data of the Global School-based Student Health Survey, this study analyzed self-reported data from adolescents in 68 countries. Multivariate logistic regression and meta-analysis were used to evaluate the association between FI and sleep disturbance. The final sample included 223,561 adolescents. The prevalence of severe FI and sleep disturbance was 6.4% and 8.0%, respectively. Severe FI was significantly associated with a higher risk of sleep disturbance in 48 of the 68 countries after adjusting for covariates, with a pooled OR (95% CI) of 1.94 (1.79–2.09). Overall, the association between FI and sleep disturbance was similar across countries and gender, though a large level of heterogeneity existed across upper- and middle-income countries. Identifying adolescents suffering from FI and remedying the FI severity may be important to improve sleep quality in global adolescents.
Machine Learning: A Catalyst for THz Wireless Networks
Authors: Boulogeorgos Alexandros-Apostolos A. , Yaqub Edwin , di Renzo Marco , Alexiou Angeliki , Desai Rachana , Klinkenberg Ralf
Published: October 2021

With the vision to transform the current wireless network into a cyber-physical intelligent platform capable of supporting bandwidth-hungry and latency-constrained applications, both academia and industry turned their attention to the development of artificial intelligence (AI) enabled terahertz (THz) wireless networks. In this article, we list the applications of THz wireless systems in the beyond fifth generation era and discuss their enabling technologies and fundamental challenges that can be formulated as AI problems. These problems are related to physical, medium/multiple access control, radio resource management, network and transport layer. For each of them, we report the AI approaches, which have been recognized as possible solutions in the technical literature, emphasizing their principles and limitations. Finally, we provide an insightful discussion concerning research gaps and possible future directions.
Conventional and UAV-Based Aerial Surveys for Long-Term Monitoring (1954–2020) of a Highly Active Rock Glacier in Austria
Authors: Kaufmann Viktor , Kellerer-Pirklbauer Andreas , Seier Gernot
Published: October 2021

Rock glaciers are creep phenomena of mountain permafrost. Speed-up has been observed on several rock glaciers in recent years and attributed to climate change. Although rare, related long-term studies are nevertheless essential to bring a climate perspective to creep velocity changes. In the present study, we focused on changes both in the surface creep velocity and volume of the Leibnitzkopf rock glacier (Hohe Tauern Range, Austria) in the period 1954–2020. We applied 3D change detection using aerial images of both conventional (12 epochs between 1954 and 2018) and unmanned aerial vehicle (UAV)-based aerial surveys (2 epochs, 2019 and 2020), and combined this with ground and air temperature data. Photogrammetric processing (structure-from-motion, multi-view stereo) of the multi-temporal dataset resulted in high-resolution digital orthophotos/DOPs (5–50 cm spatial resolution) and digital elevation models/DEMs (10–50 cm grid spacing). Georeferencing was supported by five externally triangulated images from 2018, bi-temporal aerial triangulation of the image data relying on stable ground around the rock glacier, measured ground control points (2019 and 2020), and measured camera locations (PPK-GNSS) of the UAV flight in 2020. 2D displacement vectors based on the multi-temporal DOPs and/or DEMs were computed. Accuracy analyses were conducted based on geodetic measurements (2010–2020) and airborne laser scanning data (2009). Our analyses show high multi-annual and inter-annual creep velocity variabilities with maxima between 12 (1974–1981) and 576 cm/year (2019–2020), always detected in the same area of the rock glacier where surface disintegration was first observed in 2018. Our volume change analyses of the entire landform for the period 1954–2018 do not indicate any significant changes. This suggests little permafrost ice melt and/or general low ice content of the rock glacier. Analyses of the temperature data reveal a close relationship between higher temperatures and rock glacier acceleration despite the high probability of low ice content. This suggests that hydrogeological changes play an important role in the rock glacier system. The paper concludes with a summary of technical improvements and recommendations useful for rock glacier monitoring and a general view on the kinematic state of the Leibnitzkopf rock glacier.
Efficacy of Nutrient Management Options for Finger Millet Production on Degraded Smallholder Farms in Eastern Uganda
Authors: Ebanyat Peter , de Ridder Nico , Bekunda Mateete , Delve Robert J. , Giller Ken E.
Published: October 2021

Poor soil fertility is a major problem constraining crop productivity in smallholder farms of sub-Saharan Africa due to inadequate nutrient replenishment. Differential management of nutrients creates areas of accumulation and depletion of nutrients within farms with the latter increasing in spatial coverage. Nutrient additions are required to increase crop production in such degraded areas. We used experimental data to evaluate the potential of inorganic fertilizers and organic manures to offset finger millet yield differences or gap between degraded fields and former kraals, which are recognized as niches for obtaining the best yields within the Teso farming system in eastern Uganda. Nitrogen (N) and phosphorus (P) fertilizers were sole applied at 0, 30, 60, and 90 kg ha−1 and in combination (N+P) at equal rates of sole application, and manure (3 t ha−1) supplemented with N (0, 30, 60, and 90 kg ha−1) to degraded fields located in upper and middle landscape positions in Chelekura and Onamudian villages. A second control treatment of finger millet grown on soils of former kraal sites (high fertility niches) was included as a benchmark to evaluate the efficacy of nutrient management options on degraded field. Average grain yield ranged from 404 to 2,026 kg ha−1 and differed significantly (p < 0.001) between villages and seasons. Significant effects (p < 0.05) of landscape position on grain yield were observed only in Onamudian village. Although the treatments significantly increased millet yields on degraded fields above the control, they could not eliminate the yield differences between degraded fields and former kraals. The largest average grain yields on degraded fields were obtained from combined application of N+P resulting in average grain yields of 800 and 1,171 kg ha−1 in Chelekura village and Onamudian village, respectively. These yield responses resulted in only 24 and 43% of yields obtained on former kraal fields in Chelekura and Onamudian, respectively. The physiological efficiencies, agronomic efficiencies, and apparent recoveries of N and P were low; often <25%. Pot experiments conducted in a greenhouse showed that Sulphur (S) and potassium (K) were additional limiting nutrients to N and P for finger millet production in Chelekura and Onamudian and may partly explain the large yield differences of finger millet between fertilized fields and former kraals in the smallholder farming systems. Nutrient management strategies for sustainable millet production in these farming systems need consideration of site-specific nutrient limitations.
Integrated Bioplant and Groundnut Husk Biochar Compost Application on Yield of Lettuce in a Rhodic Kandiustalf
Authors: Nartey Eric Kwesi , Darko Daniel Amoako , Sulemana Nasirudeen
Published: October 2021

Bioplant is a liquid soil conditioner that contains a consortium of beneficial fungi and bacteria manufactured by Artemis and Angel Company Limited in Bangkok. Bioplant is purported to stimulate beneficial microbial activity in soil and hence increase crop yield. However, the efficacy of Bioplant has not been evaluated on Ghanaian soils. A screen house trial was consequently conducted to evaluate the efficacy or otherwise of Bioplant on the yield of lettuce in a Rhodic Kandiustalf amended with or without compost. The soil was mixed with compost at 20 parts soil to 80 parts compost and 60 parts soil to 40 parts compost (v/v) and potted in 1.7-L pots. There was another potted soil with no compost amendment. To each of these potted soils, Bioplant was applied at four rates, viz., zero, half the manufacturer's recommended rate, the manufacturer's recommended rate, and twice the manufacturer's recommended rate, and allowed to equilibrate for 2 weeks. Seedlings of lettuce of the variety Eden were transplanted into the pots, and the treatments kept at 80% field capacity. The treatments were replicated four times in a completely randomized design. At physiological maturity, the lettuce was harvested, and fresh and dry matter yields were taken. The C and N contents and N uptake in the harvested plants were also determined. Results indicate that conditioning the soil with Bioplant at half and the manufacturer's recommended rates increased N uptake, resulting in higher carbon accumulation with concomitant increases in both fresh and dry matter yields. The results also show that amending the Rhodic Kandiustalf with Bioplant at twice the manufacturer's rate suppressed yield. Application of Bioplant at the manufacturer's recommended rate in combination with compost amended at 40 parts to 60 parts soil (v/v) saw a 47 and 90% respective significant yield increases in fresh weight and dry matter when only Bioplant was applied at the manufacturer's recommended rate. It is therefore recommended for Bioplant to be applied at the manufacturer's recommended rate of 825 mL/ha in combination with 40 parts of compost to 60 parts of soil (v/v).
Understanding Variable Motor Responses to Direct Electrical Stimulation of the Human Motor Cortex During Brain Surgery
Authors: Aaronson Daniel M. , Martinez Del Campo Eduardo , Boerger Timothy F. , Conway Brian , Cornell Sarah , Tate Matthew , Mueller Wade M. , Chang Edward F. , Krucoff Max O.
Published: October 2021

Direct electrical stimulation of the brain is the gold standard technique used to define functional-anatomical relationships during neurosurgical procedures. Areas that respond to stimulation are considered “critical nodes” of circuits that must remain intact for the subject to maintain the ability to perform certain functions, like moving and speaking. Despite its routine use, the neurophysiology underlying downstream motor responses to electrical stimulation of the brain, such as muscle contraction or movement arrest, is poorly understood. Furthermore, varying and sometimes counterintuitive responses can be seen depending on how and where the stimulation is applied, even within the human primary motor cortex. Therefore, here we review relevant neuroanatomy of the human motor system, provide a brief historical perspective on electrical brain stimulation, explore mechanistic variations in stimulation applications, examine neurophysiological properties of different parts of the motor system, and suggest areas of future research that can promote a better understanding of the interaction between electrical stimulation of the brain and its function.
Remote Training of Functional Endoscopic Sinus Surgery With Advanced Manufactured 3D Sinus Models and a Telemedicine System
Authors: Suzuki Masanobu , Vyskocil Erich , Ogi Kazuhiro , Matoba Kotaro , Nakamaru Yuji , Homma Akihiro , Wormald Peter J. , Psaltis Alkis J.
Published: October 2021

Objective: Traditionally, cadaveric courses have been an important tool in surgical education for Functional Endoscopic Sinus Surgery (FESS). The recent COVID-19 pandemic, however, has had a significant global impact on such courses due to its travel restrictions, social distancing regulations, and infection risk. Here, we report the world-first remote (Functional Endoscopic Sinus Surgery) FESS training course between Japan and Australia, utilizing novel 3D-printed sinus models. We examined the feasibility and educational effect of the course conducted entirely remotely with encrypted telemedicine software.

Methods: Three otolaryngologists in Hokkaido, Japan, were trained to perform frontal sinus dissections on novel 3D sinus models of increasing difficulty, by two rhinologists located in Adelaide, South Australia. The advanced manufactured sinus models were 3D printed from the Computed tomography (CT) scans of patients with chronic rhinosinusitis. Using Zoom and the Quintree telemedicine platform, the surgeons in Adelaide first lectured the Japanese surgeons on the Building Block Concept for a three Dimensional understanding of the frontal recess. They in real time directly supervised the surgeons as they planned and then performed the frontal sinus dissections. The Japanese surgeons were asked to complete a questionnaire pertaining to their experience and the time taken to perform the frontal dissection was recorded. The course was streamed to over 200 otolaryngologists worldwide.

Results: All dissectors completed five frontal sinusotomies. The time to identify the frontal sinus drainage pathway (FSDP) significantly reduced from 1,292 ± 672 to 321 ± 267 s (p = 0.02), despite an increase in the difficulty of the frontal recess anatomy. Image analysis revealed the volume of FSDP was improved (2.36 ± 0.00 to 9.70 ± 1.49 ml, p = 0.014). Questionnaires showed the course's general benefit was 95.47 ± 5.13 in dissectors and 89.24 ± 15.75 in audiences.

Conclusion: The combination of telemedicine software, web-conferencing technology, standardized 3D sinus models, and expert supervision, provides excellent training outcomes for surgeons in circumstances when classical surgical workshops cannot be realized.
Unequal Impact of COVID-19 on Private and Academic Neurosurgical Workforce: Results of an International Survey
Authors: Ridwan Sami , Ganau Mario , Zoia Cesare , Broekman Marike , Grote Alexander , Clusmann Hans
Published: October 2021

Background: Since the COVID-19 outbreak several manuscripts regarding neurosurgical practice during this pandemic have been published. Qualitative studies on how the pandemic affected neurosurgeons, with additional focus on their practice, are still scarce. This study's objective was to investigate the impact of COVID-19 on various aspects of the professional and private life of a homogeneous group of international neurosurgeons affiliated to the European Association of Neurosurgical Societies (EANS).

Methods: Neurosurgeons from Europe and abroad were invited to participate in an online survey endorsed by the Individual Membership Committee of the EANS. The survey captured a subjective snapshot of the impact of the first wave of the COVID-19 pandemic on EANS members and was advertised through its Institutional website. In addition to departmental data, personal feeling of safety, financial security, local precautions, number of surgeries performed, changes in daily routine, and other practice-related information were inquired. Differences among practice types were closely reviewed.

Results: The survey was distributed between April and May 2020: 204 neurosurgeons participated. Participants were typically active EANS members (73%), consultants (57.9%), from university hospitals (64.5%). Elective surgical practice was still ongoing only for 15% of responders, whereas 18.7% of them had already transitioned to COVID-19 and emergency medical services. While 65.7% of participants thought their institutions were adequately prepared, lack of testing for SARS-CoV-2, and scarcity of personal protective equipment were still a matter of concern for most of them. Overall surgical activity dropped by 68% (cranial by 54%, spine by 71%), and even emergencies decreased by 35%. COVID-19 prompted changes in communication in 74% of departments, 44% increased telemedicine by >50%. While most neurosurgeons had concerns about personal and families' health, financial outlook appeared to be gloomy only for private practitioners.

Conclusion: The lockdown imposed in many countries by the COVID-19 outbreak called for immediate modification of working routine and resulted in a dramatic decrease of elective surgical procedures. Neurosurgeons share common concerns but were not equally exposed to the personal health and financial dangers of the ongoing pandemic.
Molecular-Based Detection of Enterocytozoon bieneusi in Farmed Masked Palm Civets (Paguma larvata) in Hainan, China: A High-Prevalence, Specificity, and Zoonotic Potential of ITS Genotypes
Authors: Zhao Wei , Ren Guang-Xu , Qiang Yu , Li Jiaqi , Pu Jinkang , Zhang Yun , Tan Feng , Huang Huicong , Liang Shaohui , Lu Gang
Published: October 2021

Enterocytozoon bieneusiis a microsporidian and zoonotic species. This study investigated the prevalence and distribution ofE. bieneusigenotypes in farmed masked palm civets using nested PCR, as well as assessed their zoonotic potential by phylogenetic analysis of the ITS region of the rRNA region. Here, we collected 251 fecal specimens from farmed masked palm civets (Paguma larvata) from the Hainan Island, China. In total, 128 of 251 samples were positive forE. bieneusi, with an average infection rate of 51.0%. Seventeen genotypes were identified including 12 known genotypes—HNR-VI (n= 56), SHR1 (n= 45), SHW7 (n= 6), KIN-1 (n= 3), D (n= 3), New1 (n= 3), EbpC (n= 2), CHC5 (n= 1), CHG19 (n= 1), CHN4 (n= 1), EbpA (n= 1), and Henan-III (n= 1)—and five novel genotypes (HNPL-I to HNPL-II; one each). Phylogenetic analysis categorized these genotypes into two groups. Thirteen of them were members of the zoonotic group 1, and the remaining four genotypes were in group 12. This study has shown that the infection rates ofE. bieneusiin masked palm civets from Hainan were relatively high and provide baseline data to control and prevent microsporidiosis in farm-related communities. Therefore, infections in masked palm civets with zoonotic genotypes D, EbpC, CHN4, EbpA, KIN-1, and Henan-III should be considered potential threats to public health.
Modest de novo Reactivation of Single HIV-1 Proviruses in Peripheral CD4+ T Cells by Romidepsin
Authors: Kjær Kathrine , Leth Steffen , Konrad Christina V. , Gunst Jesper D. , Nymann Rasmus , Østergaard Lars , Søgaard Ole S. , Schleimann Mariane H. , Tolstrup Martin , Denton Paul W.
Published: October 2021

A cure for human immunodeficiency virus (HIV-1) is restricted by the continued presence of a latent reservoir of memory CD4+ T cells with proviruses integrated into their DNA despite suppressive antiretroviral therapy (ART). A predominant strategy currently pursued in HIV-1 cure-related research is the “kick and kill” approach, where latency reversal agents (LRAs) are used to reactivate transcription from integrated proviruses. The premise of this approach is that “kicking” latent virus out of hiding allows the host immune system to recognize and kill infected cells. Clinical trials investigating the efficacy of LRAs, such as romidepsin, have shown that these interventions do induce transient spikes in viral RNA in HIV-1-infected individuals. However, since these trials failed to significantly reduce viral reservoir size or significantly delay time to viral rebound during analytical treatment interruptions, it is questioned how much each individual latent provirus is actually “kicked” to produce viral transcripts and/or proteins by the LRA. Here, we developed sensitive and specific digital droplet PCR-based assays with single-provirus level resolution. Combining these assays allowed us to interrogate the level of viral RNA transcripts from single proviruses in individuals on suppressive ART with or without concomitant romidepsin treatment. Small numbers of proviruses in peripheral blood memory CD4+ T cells were triggered to become marginally transcriptionally active upon romidepsin treatment. These novel assays can be applied retrospectively and prospectively in HIV-1 cure-related clinical trials to gain crucial insights into LRA efficacy at the single provirus level.
Multimodal Analysis of Eye Movements and Fatigue in a Simulated Glass Cockpit Environment
Authors: Naeeri Salem , Kang Ziho , Mandal Saptarshi , Kim Kwangtaek
Published: October 2021

Pilot fatigue is a critical reason for aviation accidents related to human errors. Human-related accidents might be reduced if the pilots’ eye movement measures can be leveraged to predict fatigue. Eye tracking can be a non-intrusive viable approach that does not require the pilots to pause their current task, and the device does not need to be in direct contact with the pilots. In this study, the positive or negative correlations among the psychomotor vigilance test (PVT) measures (i.e., reaction times, number of false alarms, and number of lapses) and eye movement measures (i.e., pupil size, eye fixation number, eye fixation duration, visual entropy) were investigated. Then, fatigue predictive models were developed to predict fatigue using eye movement measures identified through forward and backward stepwise regressions. The proposed approach was implemented in a simulated short-haul multiphase flight mission involving novice and expert pilots. The results showed that the correlations among the measures were different based on expertise (i.e., novices vs. experts); thus, two predictive models were developed accordingly. In addition, the results from the regressions showed that either a single or a subset of the eye movement measures might be sufficient to predict fatigue. The results show the promise of using non-intrusive eye movements as an indicator for fatigue prediction and provides a foundation that can lead us closer to developing a near real-time warning system to prevent critical accidents.
Spotted Wolffish Broodstock Management and Egg Production: Retrospective, Current Status, and Research Priorities
Authors: Le François Nathalie Rose , Beirão José , Superio Joshua , Dupont Cyr Bernard-Antonin , Foss Atle , Bolla Sylvie
Published: September 2021

The first artificially fertilized spotted wolffish (Anarhichas minor) eggs hatched in Norway in the mid-1990s as this species was considered by Norwegian authorities to be a top candidate species for cold-water aquaculture in the North Atlantic regions. Previous research conducted in Norway (since 1992) and Canada (since 2000), focused on identifying key biological parameters for spotted wolffish cultivation which led, respectively, to the rapid establishment of a full commercial production line in northern Norway, while Québec (Canada) is witnessing its first privately driven initiative to establish commercial production of spotted wolffish on its territory. The control of reproduction can be viewed as a major requirement to achieve the development of performant strains using genetic selection tools and/or all-year-round production to bring about maximal productivity and synchronization among a given captive population. Although the basic reproduction aspects are more understood and controlled there are still some challenges remaining involving broodstock and upscaling of operations that limit the achievement of a standardized production at the commercial level. Quality of gametes is still considered a major constraint and it can be affected by multiple factors including nutrition, environmental conditions, handling practices, and welfare status. Internal insemination/fertilization and the protracted incubation period are challenging as well as the establishment of a health monitoring program to secure large-scale operations. The profound progress achieved in the control of reproduction, sperm handling, and cryopreservation methods for this species is presented and discussed. In this review, we also go into detail over the full range of up-to-date cultivation practices involving broodstock and identify areas that could benefit from additional research efforts (i.e., broodstock nutrition, health and welfare, scaling-up egg and larval production, genetics, and development of selective breeding programs).
Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood
Authors: Wu Mingli , Tang Xiaoqin , Raza Sayed Haidar Abbas , Zhao Haidong , Li Qi , Yi Xiaohua , Althobaiti Fayez , Shukry Mustafa , Sun Xiuzhu
Published: September 2021

Transportation is a crucial phase in the beef cattle industry, and the annual losses caused by beef cattle transport stress are substantial. Because of its huge economic losses, such as lower growth rate and even death, long-distance transportation stress has attracted more attention from beef production practitioners because of its huge economic losses. Compared with the long-distance transportation stress, the short-distance transportation stress was ignored for the reason of no obvious symptoms in cattle. Our previous study showed that the disorder of B cell function could be a potential health risk after short-distance transportation. However, the transcriptome details of the changes in the cattle blood after short-distance transportation and the molecular mechanisms for the regulation of the developmental process are not clearly known. In this study, a total of 10 Qinchuan cattle were used to compare the molecular characteristics of blood before and after short-distance transportation. The miRNA-seq showed that 114 differentially expressed miRNAs (DEMs) were found (40 upregulated and 74 downregulated) between two groups before and after transportation. Furthermore, more than 90% of the miRNAs with counts of more than 10 were used to construct a co-expression network by weighted correlation network analysis (WGCNA), and four independent modules were identified. According to their relationship with 30 hub genes, the turquoise module was the key module in this study. The regulator network of hub genes and miRNAs in the turquoise module was constructed by miRNAs targeting genes predicting, and the miRNAs had targeting sites within hub genes that could be identified as hub-miRNAs. Further, it showed that CD40 and ITPKB had the same targeting miRNAs (miR-339a/b), and the newly discovered hub miRNAs filled the gaps in our previous study about the relationship between hub genes in short-distance transportation stress and provided the potential utility for predicting and treatment of short-distance transportation stress in beef cattle.
Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations
Authors: El Brini Zineb , Fassi Fihri Ouafaa , Paillot Romain , Lotfi Chafiqa , Amraoui Farid , El Ouadi Hanane , Dehhaoui Mohamed , Colitti Barbara , Alyakine Hassan , Piro Mohammed
Published: September 2021

This study reports the first equine herpesvirus-1 (EHV-1) and equine herpesvirus-4 (EHV-4) seroprevalence investigation in horse populations of Morocco in 24 years. It also aims to determine antibody titers in horses vaccinated under field conditions with a monovalent EHV-1 vaccine. Blood samples were collected from 405 horses, including 163 unvaccinated and 242 vaccinated animals. They were tested using a commercial type-specific enzyme-linked immunosorbent assay (ELISA) and a virus neutralization test (VNT). Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. In the vaccinated group, the VNT revealed a mean antibody titer of 1:49 for EHV-1 and 1:45 for EHV-4. The present study demonstrates that EHV-1 and EHV-4 are endemic in the horse populations in the north of Morocco, with prevalence differences between regions. Furthermore, horses vaccinated with a monovalent EHV-1 vaccine had low antibodies titers. This study highlights the necessity to establish and/or support efficient biosecurity strategies based on sound management of horses and characterize further and potentially improve the efficiency of the EHV vaccines and vaccination protocol used in the field.
Effect of Light Regiment on Farrowing Performance and Behavior in Sows
Authors: McLoda Shelby , Anderson Nichole C. , Earing Jennifer , Lugar Drew
Published: September 2021

The purpose of this study was to investigate the effects of light exposure on farrowing performance in sows. Thirty sows were moved to the farrowing unit at d 110 of gestation and assigned a treatment: 12 h light/12 h dark cycle (Dark) or 24 h light (Light). Treatments began upon entry into the farrowing unit. Video was recorded continuously from initiation of the treatments until completion of farrowing. Data collected included duration of farrowing, birthing interval, and behavior during farrowing. Additionally, the number of total born, liveborn, and stillborn piglets was recorded. Gestation length was different between treatments, with a shorter gestation in Dark treatment sows than Light treatment sows (116.4 vs. 117.1 ± 0.2 d, respectively; p = 0.027). The total duration of parturition and number of liveborn did not differ (p = 0.393). Number of stillborn piglets between treatments did differ (p = 0.018). Dark had more stillborns compared to Light treatment sows (1.5 vs. 0.7 ± 0.2 piglets, respectively). Neither the interval between piglets nor farrowing behavior differed between treatments (p > 0.100). The results from this experiment indicate that a sudden change in photoperiod has the potential to impact the gestation length of sows and number of stillborn pigs.
Feeding, Muscle and Packaging Effects on Lipid Oxidation and Color of Avileña Negra-Ibérica Beef
Authors: Barahona Marta , Campo Maria del Mar , Hachemi Mohammed Amine , González Maria del Mar , Olleta José Luis
Published: September 2021

In order to increase the economic profitability of Avileña-Negra Ibérica beef production, the inclusion of corn silage in total mixed rations was proposed during the fattening period. Twenty-four Avileña-Negra Ibérica breed entire young bulls were used to evaluate the effects of two feeding systems—concentrate (CON) and corn silage (SIL)—and two packaging systems—vacuum (VAC) and modified atmosphere (MAP)—on the lipid oxidation, color evolution and visual color acceptability of meat throughout display with either oxygen-permeable film (FILM) or modified atmosphere (MAP). Two muscles were assessed: Longissimus thoracis (LT) and Semitendinosus (ST). Meat from SIL-fed animals had higher TBA values than CON-fed animals after 7 days in MAP packaging. Lipid oxidation was prevented more effectively by VAC packaging than MAP. Display time highly influenced lipid oxidation, since oxidation rates were lower with VAC than MAP packaging. After 14 days of display in MAP, the LT from CON was lighter than the ST. Meat discoloration after 7 days of display was significantly different between packaging systems. Vacuum-packaged meat kept the same color throughout the storage time. Visual color acceptability for the LT was higher throughout display than for the ST. Samples from the MAP were well accepted by consumers, especially the meat from the SIL group. Alternatively, feeding with corn silage could be used for this type of animals without affecting important aspects of meat quality.
Mammary Development in Gilts at One Week Postnatal Is Related to Plasma Lysine Concentration at 24 h after Birth, but Not Colostrum Dose
Authors: Bitsie Bryce , Ison Erin Kay , Jenkins Leah Parker , Klopp Rebecca , McCabe Conor , Mills Kayla , Nicholls Griffin , Richards Andrew , Shirley Larissa , Teeple Kelsey , Schinckel Allan P. , Kwon Angela , Stewart Kara R. , Jannasch Amber , Suarez-Trujillo Aridany , Casey Theresa M.
Published: October 2021

Perinatal nutrition affects future milk production. The number of mammary epithelial cells affect milk production capacity. Therefore, it was hypothesized that the level of colostrum intake affects the proliferation rate and the total number of mammary epithelial cells in the gland. The ratio of newly synthesized protein to newly synthesized DNA reflects the relative amount of cellular differentiation to cell division. The study objective was to determine the relationship between the level of colostrum intake and 24 h-level of circulating amino acid, glucose and insulin with mammary parenchyma histological features, cell division and protein synthesis over the first week postnatal. One of two standardized doses of a homogenate colostrum sample, 10% (n = 8) and 20% (n = 8) of birth bodyweight, was fed to gilts over the first 24 h postnatal. Gilts were administered deuterium oxide immediately after birth and daily to label newly synthesized DNA and proteins. Gilts were euthanized on postnatal day seven, and DNA and protein were isolated from mammary parenchyma. DNA and protein fractional synthesis (f) and fractional synthetic rate (FSR) were calculated using mass isotopomer distribution analysis. The ratio of protein f and FSR to DNA f and FSR were calculated and used to indicate the relative amounts of differentiation to cell division. Mammary morphological development was also analyzed by measuring the parenchymal epithelial area and the stromal and epithelial proliferation index on postnatal day seven. Colostrum dose was not related to any of the variables used to evaluate mammary development. However, plasma lysine levels at 24 h postnatal were positively related to average daily gain (ADG; r = 0.54, p = 0.05), DNA f (r = 0.57; p = 0.03) and DNA FSR (r = 0.57; p = 0.03) in mammary parenchyma. Plasma lysine was inversely related to the ratio of protein to DNA f and FSR (r = −0.56; p = 0.04). ADG was related to the parenchymal epithelial area and DNA and protein f and FSR (p < 0.05). These relationships support the idea that the nutritional environment affects early mammary development and that higher lysine levels in the perinatal period favored a greater degree of cell division versus differentiation in mammary of neonatal pigs and thus, warrant further investigations.
A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep
Authors: Molina-Hernández Verónica , Ruiz-Campillo María T. , Martínez-Moreno Francisco J. , Buffoni Leandro , Martínez-Moreno Álvaro , Zafra Rafael , Bautista María J. , Escamilla Alejandro , Pérez-Caballero Raúl , Pérez José
Published: October 2021

Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial.
Transcriptional Reprogramming in Rumen Epithelium during the Developmental Transition of Pre-Ruminant to the Ruminant in Cattle
Authors: Baldwin VI Ransom L. , Liu Mei , Connor Erin E. , Ramsay Timothy G. , Liu George E. , Li Cong-Jun
Published: October 2021

We present an analysis of transcriptomic dynamics in rumen epithelium of 18 Holstein calves during the transition from pre-rumination to rumination in cattle-fed hay or concentrated diets at weaning. Three calves each were euthanized at 14 and 42 d of age to exemplify preweaning, and six calves each were provided diets of either milk replacer and grass hay or calf starter to introduce weaning. The two distinct phases of rumen development and function in cattle are tightly regulated by a series of signaling events and clusters of effectors on critical pathways. The dietary shift from liquid to solid feeds prompted the shifting of gene activity. The number of differentially expressed genes increased significantly after weaning. Bioinformatic analysis revealed gene activity shifts underline the functional transitions in the ruminal epithelium and signify the transcriptomic reprogramming. Gene ontogeny (GO) term enrichment shows extensively activated biological functions of differentially expressed genes in the ruminal epithelium after weaning were predominant metabolic functions. The transcriptomic reprogramming signifies a correlation between gene activity and changes in metabolism and energy production in the rumen epithelium, which occur at weaning when transitioning from glucose use to VFA use by epithelium during the weaning.
Environmental DNA as Novel Technology: Lessons in Agenda Setting and Framing in News Media
Authors: Fitzgerald Amy , Halliday Jennifer , Heath Daniel
Published: October 2021

Environmental DNA (eDNA) is an emerging technology used for understanding ecosystems, environmental change, and stressors. Cellular and extracellular DNA are collected from environmental samples instead of individual wildlife animals, and as such eDNA comes with associated logistical and ethical benefits. It is increasingly being used, yet to date public knowledge and perceptions of eDNA have not been explored. Given that most of the public gathers scientific information from news media sources, this is a logical first place to start. This paper reports on a framing and agenda-setting analysis of news media coverage of eDNA in Canada and the United States from 2000 to 2020. The findings indicate that eDNA is being framed as an emerging and powerful tool, although questions regarding its validity and reliability are raised vis-à-vis identifying the presence of invasive species. Less than half of the news articles analyzed address broader social or ethical issues in relation to eDNA, and the majority focus on the potential financial impacts of eDNA findings on development projects and business interests. The potential ethical advantages of non-lethal sampling methods used via eDNA sampling are not addressed, nor are the potential ethical issues raised by its potential use in bioprospecting, indicating that the current state of agenda setting regarding eDNA in these newspapers is focused on economic impacts, to the exclusion of potential ethical issues. This unfolding news coverage will likely be key to understanding public perceptions of this novel technology.
Guidelines for the Measurement of Rein Tension in Equestrian Sport
Authors: Clayton Hilary , MacKechnie-Guire Russell , Byström Anna , Le Jeune Sarah , Egenvall Agneta
Published: October 2021

Rein tension is relatively easy to measure, and the resulting data are useful for evaluating the interaction between horse and rider. To date, there have been a number of studies using different transducers, calibration methods and analytical techniques. The purpose of this paper is to make recommendations regarding the collection, analysis and reporting of rein tension data. The goal is to assist users in selecting appropriate equipment, choosing verified methods of calibration, data collection and analysis, and reporting their results consistently to facilitate comparisons between different studies. Sensors should have a suitable range and resolution together with a fast enough dynamic response, according to the gait, speed and type of riding for which they will be used. An appropriate calibration procedure is necessary before each recording session. A recording frequency of 50 Hz is adequate for most rein tension studies. The data may be analyzed using time-series methods or by extracting and analyzing discrete variables chosen in accordance with the study objectives. Consistent reporting facilitates comparisons between studies.