bluuebunny/crossref_metadata_2025 huggingface dataset:
Phase I Study of a Combination of Fluvastatin and Celecoxib in Children with Relapsing/Refractory Low-Grade or High-Grade Glioma (FLUVABREX)
Authors: Leblond Pierre , Tresch-Bruneel Emmanuelle , Probst Alicia , Néant Nadège , Solas Caroline , Sterin Arthur , Boulanger Thomas , Aerts Isabelle , Faure-Conter Cécile , Bertozzi Anne-Isabelle , Chastagner Pascal , Entz-Werlé Natacha , De Carli Emilie , Deley Marie-Cécile Le , Bouche Gauthier , André Nicolas
Published: March 2023

Preclinical data support the activity of celecoxib and fluvastatin in high-grade (HGG) and low-grade gliomas (LGG). A phase I trial (NCT02115074) was designed to evaluate the safety of this combination in children with refractory/relapsed HGG and LGG using four dose levels of fluvastatin with a fixed daily dose of celecoxib. A Continual Reassessment Method was used for fluvastatin dose escalation. Dose-limiting toxicities (DLT) were determined on the first treatment cycle. Twenty patients were included. Ten LGG and ten HGG patients received a median of 3.5 treatment cycles. Two DLTs were reported: one grade 3 maculopapular rash (4 mg/kg dose level) and one grade 4 increase of Creatine Phospho-Kinase (6 mg/kg dose level). We identified the dose of 6 mg/kg/day as the recommended phase II dose (RP2D) of fluvastatin with celecoxib. Four patients with LGG continued treatment beyond 12 cycles because of stable disease, including one patient who received 23 treatment cycles. In children with refractory/relapsed glioma, the RP2D of fluvastatin with celecoxib is 6 mg/kg/day. The long-term stable diseases observed in LGG suggest a possible role of the combination in a maintenance setting, given its good tolerance and low cost for children living in low- and middle-income countries.
Prognostic Value of Inflammatory and Nutritional Biomarkers of Immune Checkpoint Inhibitor Treatment for Recurrent or Metastatic Squamous Cell Carcinoma of the Head and Neck
Authors: Sakai Akihiro , Iijima Hiroaki , Ebisumoto Koji , Yamauchi Mayu , Teramura Takanobu , Yamazaki Aritomo , Watanabe Takane , Inagi Toshihide , Maki Daisuke , Okami Kenji
Published: March 2023

This study aimed to determine the prognostic value of inflammatory and nutritional biomarkers of immune checkpoint inhibitor (ICI) therapy for recurrent or metastatic squamous cell carcinoma of the head and neck (RMHNSCC) and to identify the most useful factor for prognosis assessment. We retrospectively reviewed the medical records of patients with RMHNSCC who received ICI therapy. The response rate for ICI therapy and the relationship between inflammatory and nutritional biomarkers and overall survival were examined. The included biomarkers did not correlate with an objective response rate but were associated with a disease control rate. Univariate analysis showed significant correlations between the serum albumin level, C-reactive protein level, platelet to lymphocyte ratio, neutrophil to lymphocyte ratio, lymphocyte to monocyte ratio (LMR), systemic immune-inflammation index, and controlling the nutritional status score and overall survival; multivariate analysis showed that LMR was significantly correlated with overall survival. LMR was the most important biomarker according to the machine learning model. This study suggests that LMR may be the most useful biomarker for predicting the prognosis of ICI treatment for RMHNSCC.
The Glioma Immune Landscape: A Double-Edged Sword for Treatment Regimens
Authors: Mahajan Sukrit , Schmidt Mirko H. H. , Schumann Ulrike
Published: March 2023

Immune cells constitute a major part of the tumor microenvironment, thereby playing an important role in regulating tumor development. They interact with tumor cells, resulting in the suppression or promotion of glioma development. Therefore, in recent years, scientists have focused on immunotherapy that involves enhancing the immune response to fight the battle against cancer more effectively. While it has shown success against different cancer types, immunotherapy faces major roadblocks in glioma treatment. These involve the blood brain barrier, tumor heterogeneity and an immunosuppressive glioma microenvironment, among other factors. Additionally, the interaction of the peripheral immune system with the central nervous system provides another challenge for immunotherapeutic regimens. For modulating different immune cell populations to counter glioma cells, it is important to expand our knowledge about their role within the glioma microenvironment; therefore, herein, we review the different immune cell populations found in the glioma microenvironment and navigate through the various shortcomings of current immunotherapies for glioma. We conclude by providing an insight into ongoing pre-clinical and clinical trials for glioma therapies.
Recurrent Glioblastoma: What Is the Route?
Authors: Bosio Alberto , Lombardi Giuseppe
Published: March 2023

Glioblastoma (GBM) is the most frequent and aggressive malignant primary central nervous system tumor in adults [...]
Optimization Study on Synergistic System of Photocatalytic Degradation of AR 26 and UV-LED Heat Dissipation
Authors: Wang Chen , Bai Haoliang , Kang Xue
Published: March 2023

In this work, a novel UV-LED/TiO2 photocatalytic system, having a single layer with ten LED beads, was designed to simultaneously achieve UV-LED cooling and wastewater degradation, to deal with heat dissipation problems of high-power UV-LEDs. To gain more insight into this system, the parameters affecting both cooling and photocatalytic performance were first optimized using AR 26 as a basis. With respect to sewage, sewage with a flow rate of 80 mL/min and a temperature of 20 °C helped to keep a lower temperature of UV-LED, which benefits the long-term operation stability of LED beads. For parameters affecting the photocatalytic performance only, the experiments showed that TiO2 with moderate dosing (0.75 g/L) under strong acid conditions (pH = 2) helped to further improve photocatalytic activity when the initial concentration of AR 26 was 45 mg/L. Lastly, to illustrate the advantages of this novel system, the performance of the synergistic system was compared with a conventional photocatalytic reactor with respect to degradation performance, optical quantum efficiency, and energy consumption. The results showed that the degradation efficiency and light source utilization ratio of this coupled system were, respectively, 2.1 times and 1.5 times as much as those of a conventional reactor. As the unit power consumption of the synergistic system was only 0.18-fold more than that of a conventional reactor, our work suggests that this synergistic system with the advantage of LED lamp beads has a bright future in dealing with refractory organic pollutants of sewage.
3D Flower-like TiO2 Nanostructures: Anatase-To-Rutile Phase Transformation and Photoelectrochemical Application
Authors: Kusior Anna , Jeleń Piotr , Sitarz Maciej , Świerczek Konrad , Radecka Marta
Published: March 2023

Flower-like TiO2 nanostructures were obtained by chemical oxidation of Ti foil using H2O2 combined with subsequent annealing. This paper offers an analysis of the phase transformation of 3D flower-like titanium dioxide nanostructures. The role of the annealing atmosphere, sample thickness, grain shape, and nanoflower size are discussed. The nanostructures were examined using SEM, XRD, and Raman spectroscopy. Due to the nature of these two processes, the morphology of these nanomaterials is complex, and is obtained through a reaction involving Ti foil and H2O2 at 80°C. A distinction is made between the layer composed of small grains at the substrate/oxide interface, elongated crystal-like structures, and outer spongle-like film. The annealing parameters, such as atmosphere (air or argon) and temperature (450 or 600 °C), affect phase composition. The photoelectrochemical performance of the anode based on flower-like TiO2 has been shown. The thickness and phase composition of the anodes are factors that strongly affect the photocurrent. The multiphase heterojunctions proposed for 3D flower-like TiO2 photoanodes in photoelectrochemical (PEC) cells suppose that the conduction band of anatase should be above rutile. The highest photoelectrochemical performance was obtained for a photoanode composed of 20–40% anatase and an associated thickness of 0.75–1.5 µm.
Insights into the Modifying Effect of Ga on Cu-Based Catalysts for Hydrogenation of Hydroxypivalaldehyde to Neopentyl Glycol
Authors: Zhu Jinghu , Liu Hui , Zhou Xueyan , Wang Zhijun , Bing Liancheng , Han Dezhi , Wang Guangjian , Wang Fang
Published: March 2023

Cu-based catalysts, modified by gallium addition via the stepwise co-precipitation method, were studied for the liquid phase hydrogenation of hydroxypivalaldehyde (HPA) to neopentyl glycol (NPG). Through physico-chemical techniques, the effects of gallium introduction on the Cu trimetallic catalyst performance and the reaction mechanism of HPA hydrogenation were discussed. The characterization results showed that gallium introduction can influence the dispersion, reduction, and distribution of active Cu species, as well as their reactivity. Herein, the catalyst with 2 wt% gallium addition exhibited excellent catalytic performance with HPA conversion rate and NPG selectivity of 93.5% and 95.5%, at a reaction pressure of 3 MPa, temperature of 110 °C, hydrogen-aldehyde ratio (molar ratio) 10:1, and liquid space-time at a speed of 8.4 h−1. The good performance could be attributed to gallium doping tending to dynamically tune the interaction between the components, increasing Cu dispersion and the distributions of Cu+ and Cu0 species on the catalyst surfaces.
Chromatin Remodeling Enzyme Snf2h Is Essential for Retinal Cell Proliferation and Photoreceptor Maintenance
Authors: Kuzelova Andrea , Dupacova Naoko , Antosova Barbora , Sunny Sweetu Susan , Kozmik Zbynek , Paces Jan , Skoultchi Arthur I. , Stopka Tomas , Kozmik Zbynek
Published: March 2023

Chromatin remodeling complexes are required for many distinct nuclear processes such as transcription, DNA replication, and DNA repair. However, the contribution of these complexes to the development of complex tissues within an organism is poorly characterized. Imitation switch (ISWI) proteins are among the most evolutionarily conserved ATP-dependent chromatin remodeling factors and are represented by yeast Isw1/Isw2, and their vertebrate counterparts Snf2h (Smarca5) and Snf2l (Smarca1). In this study, we focused on the role of the Snf2h gene during the development of the mammalian retina. We show that Snf2h is expressed in both retinal progenitors and post-mitotic retinal cells. Using Snf2h conditional knockout mice (Snf2h cKO), we found that when Snf2h is deleted, the laminar structure of the adult retina is not retained, the overall thickness of the retina is significantly reduced compared with controls, and the outer nuclear layer (ONL) is completely missing. The depletion of Snf2h did not influence the ability of retinal progenitors to generate all the differentiated retinal cell types. Instead, the Snf2h function is critical for the proliferation of retinal progenitor cells. Cells lacking Snf2h have a defective S-phase, leading to the entire cell division process impairments. Although all retinal cell types appear to be specified in the absence of the Snf2h function, cell-cycle defects and concomitantly increased apoptosis in Snf2h cKO result in abnormal retina lamination, complete destruction of the photoreceptor layer, and consequently, a physiologically non-functional retina.
The Impact of Phase-Specific Macrophage Depletion on Intestinal Anastomotic Healing
Authors: Winter Maximiliane , Heitplatz Barbara , Koppers Nils , Mohr Annika , Bungert Alexander D. , Juratli Mazen A. , Strücker Benjamin , Varga Georg , Pascher Andreas , Becker Felix
Published: March 2023

Intestinal anastomotic healing (AH) is critical in colorectal surgery, since disruptive AH leads to anastomotic leakage, a feared postoperative complication. Macrophages are innate immune cells and are instrumental in orchestrating intestinal wound healing, displaying a functional dichotomy as effectors of both tissue injury and repair. The aim of this study was to investigate the phase-specific function and plasticity of macrophages during intestinal AH. Transgenic CD11b diphtheria toxin receptor (CD11b-DTR) mice were used to deplete intestinal macrophages in a temporally controlled manner. Distal colonic end-to-end anastomoses were created in CD11b-DTR, and wild-type mice and macrophages were selectively depleted during either the inflammatory (day 0–3), proliferative (day 4–10), or reparative (day 11–20) phase of intestinal AH, respectively. For each time point, histological and functional analysis as well as gene set enrichment analysis (GSEA) of RNA-sequencing data were performed. Macrophage depletion during the inflammatory phase significantly reduced the associated inflammatory state without compromising microscopic AH. When intestinal macrophages were depleted during the proliferative phase, AH was improved, despite significantly reduced perianastomotic neoangiogenesis. Lastly, macrophages were depleted during the reparative phase and GSEA revealed macrophage-dependent pathways involved in collagen remodeling, cell proliferation, and extracellular matrix composition. However, AH remained comparable at this late timepoint. These results demonstrate that during intestinal AH, macrophages elicit phase-specific effects, and that therapeutic interventions must critically balance their dual and timely defined role.
Multiple Forms of Neural Cell Death in the Cyclical Brain Degeneration of A Colonial Chordate
Authors: Anselmi Chiara , Caicci Federico , Bocci Tommaso , Guidetti Matteo , Priori Alberto , Giusti Veronica , Levy Tom , Raveh Tal , Voskoboynik Ayelet , Weissman Irving L. , Manni Lucia
Published: March 2023

Human neuronal loss occurs through different cellular mechanisms, mainly studied in vitro. Here, we characterized neuronal death in B. schlosseri, a marine colonial tunicate that shares substantial genomic homology with mammals and has a life history in which controlled neurodegeneration happens simultaneously in the brains of adult zooids during a cyclical phase named takeover. Using an ultrastructural and transcriptomic approach, we described neuronal death forms in adult zooids before and during the takeover phase while comparing adult zooids in takeover with their buds where brains are refining their structure. At takeover, we found in neurons clear morphologic signs of apoptosis (i.e., chromatin condensation, lobed nuclei), necrosis (swollen cytoplasm) and autophagy (autophagosomes, autolysosomes and degradative multilamellar bodies). These results were confirmed by transcriptomic analyses that highlighted the specific genes involved in these cell death pathways. Moreover, the presence of tubulovesicular structures in the brain medulla alongside the over-expression of prion disease genes in late cycle suggested a cell-to-cell, prion-like propagation recalling the conformational disorders typical of some human neurodegenerative diseases. We suggest that improved understanding of how neuronal alterations are regulated in the repeated degeneration–regeneration program of B. schlosseri may yield mechanistic insights relevant to the study of human neurodegenerative diseases.
Cellular Distribution of Secreted Phospholipase A2 in Lungs of IPF Patients and Its Inhibition in Bleomycin-Induced Pulmonary Fibrosis in Mice
Authors: Jaiswal Ashish , Rehman Rakhshinda , Dutta Joytri , Singh Sabita , Ray Archita , Shridhar Malathy , Jaisankar Jaswant , Bhatt Manas , Khandelwal Dikshit , Sahoo Bandya , Ram Arjun , Mabalirajan Ulaganathan
Published: March 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease with a very poor prognosis as it has a 2.5 to 5 years mean survival after proper diagnosis. Even nintedanib and pirfenidone cannot halt the progression, though they slow the progression of IPF. Hence, there is a need to understand the novel pathophysiology. Phospholipase A2 (PLA2) could be the ideal candidate to study in IPF, as they have a role in both inflammation and fibrosis. In the present study, we have shown the expression profile of various secretory Phospholipase A2 (PLA2) isoforms by analyzing publicly available transcriptome data of single cells from the lungs of healthy individuals and IPF patients. Among 11 members of sPLA2, PLA2G2A is found to be increased in the fibroblasts and mesothelial cells while PLA2G5 is found to be increased in the fibroblasts of IPF patients. We identified a subset of fibroblasts expressing high PLA2G2A with moderate expression of PLA2G5 and which are specific to IPF only; we named it as PLA2G2A+ IPF fibroblast. Pathway analysis revealed that these PLA2G2A+ IPF fibroblast have upregulation of both inflammatory and fibrosis-related pathways like the TGF-β signaling pathway, IL-17 signaling, the arachidonic acid metabolism pathway and ECM-receptor interaction. In addition to this, we found elevated levels of sPLA2-IIA in plasma samples of IPF patients in our cohort. PLA2G3, PLA2G10 and PLA2G12B are found in to be increased in certain epithelial cells of IPF patients. Thus, these findings indicate that these five isoforms have a disease-dominant role along with innate immune roles as these isoforms are found predominantly in structural cells of IPF patients. Further, we have targeted sPLA2 in mice model of bleomycin-induced lung fibrosis by pBPB, a known sPLA2 inhibitor. pBPB treatment attenuated lung fibrosis induced by bleomycin along with a reduction in TGF-β and deposition of extracellular matrix in lung. Thus, these findings indicate that these sPLA2 isoforms especially PLA2G2A may serve as a therapeutic target in lung fibrosis.
Mucopolysaccharidosis Type I in Mexico: Case-Based Review
Authors: Cantú-Reyna Consuelo , Vazquez-Cantu Diana Laura , Cruz-Camino Héctor , Narváez-Díaz Yuriria Arlette , Flores-Caloca Óscar , González-Llano Óscar , Araiza-Lozano Carolina , Gómez-Gutiérrez René
Published: March 2023

Introduction: Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disease present in 1:100,000 newborns. Variants in the IDUA (alpha-L-iduronidase) gene decrease the enzyme activity for glycosaminoglycans metabolism. MPS I patients exhibit clinical manifestations that fall on the Hurler, Hurler–Scheie, and Scheie syndrome spectrum. Case presentation: We present a male Mexican patient with respiratory exacerbations requiring recurrent hospitalizations. He showed macrocephaly, coarse facies, hepatomegaly, umbilical hernia, and dorsal kyphosis. The sequencing of the IDUA gene revealed the following genotype: c.46_57del12/c.1205G>A. He received combined therapy with hematopoietic stem cell transplantation and enzyme replacement. Mexican case reports were analyzed to estimate the prevalence of the associated genetic variants. Conclusion: Despite the challenges of managing this rare disease in Mexico, our patient benefited from the combined therapy. The discrete clinical manifestations and prompt evaluation by a geneticist were crucial in establishing a diagnosis, enabling an early intervention by a multidisciplinary team. The combination of ERT before and after HSCT provided health benefits to our patient.
A Survey of Current Practice in Operative Management of Testicular Torsion in Poland
Authors: Piotrowska-Gall Aneta , Stępień Piotr , Wolak Przemysław
Published: March 2023

The primary aim of this study is to evaluate heterogeneity in the current management of testicular torsion (TT). A secondary aim is to investigate incidences of recurrent torsion and the methods used for primary fixation. An online multiple-choice questionnaire comprising 10 questions was distributed to paediatric surgeons and urologists. There were a total of 99 questionnaires distributed to representatives from 39 paediatric surgery and urology departments in Poland. The majority of participants agreed on fixation of the torsed testicle (98%). Use of sutures was reported by 95% of surgeons: absorbable by 48%, non-absorbable by 42%, and 4% using both. There was no consensus on the number of sutures. The contralateral testicle was always fixed by 69%, with 28% fixing it only in case of necrosis and amputation of the torsed testicle, and the remaining 2% never fixing the contralateral side. In case of negative scrotal exploration, 18% of surgeons would still fix the testis. The recurrence of torsion after prior fixation was identified by eight of the participants. The most commonly reported primarily used technique was absorbable sutures. There is a general consensus on the fixation of torsed testicles; however, other aspects remain controversial. Based on the survey and the literature review, the use of non-absorbable sutures rather than absorbable sutures would be advisable.
A Mini-Review of Synthetic Organic and Nanoparticle Antimicrobial Agents for Coatings in Textile Applications
Authors: Karypidis Michail , Karanikas Evangelos , Papadaki Aikaterini , Andriotis Eleftherios G.
Published: March 2023

Many synthetic compounds have been applied to impart antimicrobial properties to fabrics. In this review, the types of bacteria are described. Furthermore, synthetic antimicrobial agents, namely quaternary ammonium compounds (QACs), polyhexamethylene biguanide (PHMB), triclosan, and nitrogen-halamines (N-halamines), are discussed along with their properties, their advantages and disadvantages. Although synthetic antimicrobial agents neutralise microorganisms, some adversely affect the environment, safety and health. These problems led to a novel generation of antimicrobial coating treatments on textiles, such as copper nanoparticles (CNPs) and silver nanoparticles (AgNPs) formed on plant extracts, chitosan and green synthesis, with a lower environmental impact but unaltered premium antimicrobial performance and improved durability.
An Event Study of the Ethereum Transition to Proof-of-Stake
Authors: Kapengut Elie , Mizrach Bruce
Published: March 2023

On 15 September 2022, the Ethereum network adopted a proof-of-stake (PoS) consensus mechanism. We study the impact on the network and competing platforms in a two month event window around the Beacon chain merge. We find that the transition to PoS has reduced energy consumption by 99.98%. Miners have not transformed into validators, and total block reward income (in USD) has fallen by 97%, though transaction fees (in ETH) for Ether have increased nearly 10%. The Herfindahl index for the top 10 is 1009; the network is 19% less concentrated after the merge. Ethereum supply growth has been deflationary since the merge. The time between consecutive blocks is now steady at 12 s and transactions per day are up 7.0%. On Polygon, Matic fees rose but token fees fell. Polygon also slows, processing 3.3% fewer transactions per day. Solana’s fees fall by $0.0003, and transactions per day are down 48%. Stablecoin transfer volumes fall on Ethereum and Polygon, but rise on Solana.
Chemical Characterization and Bioactive Properties of Wine Lees and Diatomaceous Earth towards the Valorization of Underexploited Residues as Potential Cosmeceuticals
Authors: Duarte Cristina N. , Taofiq Oludemi , Dias Maria Inês , Heleno Sandrina A. , Santos-Buelga Celestino , Barros Lillian , Amaral Joana S.
Published: March 2023

Annually, wine production is responsible for generating large quantities of residues, which are frequently disposed of and not valorized. So far, different studies have been conducted on grape pomace, yet less attention has been paid to other residues, such as wine lees and diatomaceous earth used in wine filtration. In this context, this study aimed to evaluate and compare the phenolic profile of these underexploited winemaking residues and assess their biological potential based on their antioxidant, antimicrobial, cytotoxic, and anti-aging activities (inhibition of tyrosinase and collagenase). Twenty-nine phenolic compounds, including twelve anthocyanins, were tentatively identified in the residues, with red grape pomace showing the highest diversity of compounds. The diatomaceous earth presented the highest content of non-anthocyanin phenolic compounds, being particularly rich in flavan-3-ols and myricetin-O-hexoside, and also presenting two anthocyanins. This sample also showed a high antioxidant activity, evidencing the best result in the reducing power assay. The red wine lees extract, despite showing a low content of phenolic compounds and less antioxidant activity, presented the highest inhibition capacity of bacteria growth. The extracts did not exhibit cytotoxicity against keratinocyte (up to 400 μg/mL) and fibroblast (up to 100 μg/mL) skin cell lines. However, the capacity of inhibiting tyrosinase and collagenase was low for the lees and diatomaceous earth, contrary to the grape pomace, seeds, and skins extracts that showed promising results, evidencing its potential as a cosmeceutical. Overall, this study highlights for the first time the potential of diatomaceous earth, an underexploited winemaking waste, in the obtention of added-value extracts and/or ingredients for cosmetic industry.
Enhanced Photodegradation of Rhodamine B Using Visible-Light Sensitive N-TiO2/rGO Composite
Authors: Utami Maisari , Wang Shaobin , Fajarwati Febi Indah , Salsabilla Siva Nur , Dewi Tania Amara , Fitri Melinda
Published: March 2023

Rhodamine B (RhB) is extensively used for dyeing purposes, and cannot be completely removed using traditional water treatment technologies. Here, we report for the first time the photodegradation of RhB using nitrogen-doped titanium dioxide (N-TiO2) on reduced graphene oxide (rGO) composite (N-TiO2/rGO). The work primarily highlights the synergistic effect of the incorporation of N-TiO2 and rGO and its kinetic study for the photodegradation of RhB. The N-TiO2/rGO composite was synthesized by dispersing titanium(IV) isopropoxide and urea, followed by annealing treatment via the hydrothermal method with rGO. Scanning electron microscopy (SEM) images illustrated that N-TiO2 particles with an irregular round shape and white color were dispersed onto the rGO surface. X-Ray diffraction (XRD) patterns revealed that N-TiO2/rGO composite showed an anatase phase of TiO2 with a diffraction peak of 2θ = 25.622°. The gas sorption analysis (GSA) showed that N-TiO2/rGO had surface area, pore volume, and pore size of 53.393 m2/g, 0.096 cc/g, and 3.588 nm, respectively. The thermogravimetric-differential thermal analysis (TG-DTA) showed an anatase phase of TiO2 that appeared at a temperature of 200–500 °C, with a weight loss of 2.50%. According to the ultraviolet-visible diffuse reflectance spectroscopy (UV-Vis DRS) study, TiO2, N-TiO2, and N-TiO2/rGO had band gap energies of 3.25, 2.95, and 2.86 eV, respectively. The highest photodegradation of RhB was obtained at the optimum condition in pH 2 with a photocatalyst mass of 20 mg and an irradiation time of 90 min. The photocatalytic activity of N-TiO2/rGO using visible light showed a higher percentage of photodegradation at 78.29%, compared to 44.08% under UV light. The kinetic study of the photodegradation of RhB using N-TiO2/rGO followed the pseudo-second-order model.
Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Is Associated with Cervical Stromal Involvement in Endometrial Cancer Patients: A Cross-Sectional Study in South China
Authors: Lin Xite , Chen Chunxia , Jiang Tingting , Ma Jincheng , Huang Lixiang , Huang Leyi , Lei Huifang , Tong Yao , Huang Guanxiang , Mao Xiaodan , Sun Pengming
Published: March 2023

Background: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a significant health issue closely associated with multiple extrahepatic cancers. The association between MAFLD and clinical outcomes of endometrial cancer (EC) remains unknown. Methods: We retrospectively included 725 EC patients between January 2012 and December 2020. The odds ratios (ORs) were calculated using logistic regression analyses. Kaplan–Meier survival curves were used for survival analysis. Results: Among EC patients, the prevalence of MAFLD was 27.7% (201/725, 95% confidence interval (Cl) = 0.245–0.311). MAFLD was significantly associated with cervical stromal involvement (CSI) (OR = 1.974, 95% confidence interval (Cl) = 1.065–3.659, p = 0.031). There was a significant correlation between overall survival (OS) and CSI (HR = 0.31; 95%CI: 0.12–0.83; p = 0.020), while patients with MAFLD had a similar OS to those without MAFLD (p = 0.952). Moreover, MAFLD was significantly associated with CSI in the type I EC subgroup (OR = 2.092, 95% confidence interval (Cl) = 1.060–4.129, p = 0.033), but not in the type II EC subgroup (p = 0.838). Further logistic regression analysis suggested that the hepatic steatosis index (HSI) was significantly associated with CSI among type I EC patients without type 2 diabetes mellitus (T2DM) (OR = 1.079, 95% confidence interval (Cl) = 1.020–1.139, p = 0.012). Conclusions: About one-quarter of our cohort had MAFLD. MAFLD was associated with the risk of CSI in EC patients, and this association existed in type I EC patients but not in type II EC patients. Furthermore, the HSI can help predict CSI in type I EC patients without T2DM.
Sentiment Analysis of Multilingual Dataset of Bahraini Dialects, Arabic, and English
Authors: Omran Thuraya , Sharef Baraa , Grosan Crina , Li Yongmin
Published: March 2023

Sentiment analysis is an application of natural language processing (NLP) that requires a machine learning algorithm and a dataset. In some cases, the dataset availability is scarce, particularly with Arabic dialects, precisely the Bahraini ones, which necessitates using an approach such as translation, where a rich source language is exploited to create the target language dataset. In this study, a dataset of Amazon product reviews in Bahraini dialects is presented. This dataset was generated using two cascading stages of translation—a machine translation followed by a manual one. Machine translation was applied using Google Translate to translate English Amazon product reviews into Standard Arabic. In contrast, the manual approach was applied to translate the resulting Arabic reviews into Bahraini ones by qualified native speakers utilizing constructed customized forms. The resulting parallel dataset of English, Standard Arabic, and Bahraini dialects is called English_Modern Standard Arabic_Bahraini Dialects product reviews for sentiment analysis “E_MSA_BDs-PR-SA”. The dataset is balanced, composed of 2500 positive and 2500 negative reviews. The sentiment analysis process was implemented using a stacked LSTM deep learning model. The Bahraini dialect product dataset can be utilized in the transfer learning process for sentimentally analyzing another dataset in Bahraini dialects.
Clinical Impact of Preoperative Biliary Drainage in Patients with Ductal Adenocarcinoma of the Pancreatic Head
Authors: Amaral Maria João , Freitas João , Amaral Mariana , Serôdio Marco , Oliveira Rui Caetano , Donato Paulo , Tralhão José Guilherme
Published: March 2023

Our aim was to study the association between preoperative biliary drainage (PBD) and morbidity following cephalic pancreaticoduodenectomy (CPD) for pancreatic ductal adenocarcinoma (PDAC) and its prognostic impact, which is still controversial in the literature. A retrospective study was conducted, which included 128 patients who underwent CPD for PDAC, divided into two groups: those who underwent PBD (group 1) and those who did not undergo this procedure (group 2). Group 1 was subdivided according to the drainage route: endoscopic retrograde cholangiopancreatography (ERCP), group 1.1, and percutaneous transhepatic cholangiography (PTC), group 1.2. 34.4% of patients underwent PBD, and 47.7% developed PBD-related complications, with 37% in group 1.1 and 64.7% in group 1.2 (p = 0.074). There was a significant difference between group 1 and 2 regarding bacterial colonization of the bile (45.5% vs. 3.6%, p < 0.001), but no difference was found in the colonization by multidrug-resistant bacteria, the development of Clavien–Dindo ≥ III complications, clinically relevant pancreatic fistula and delayed gastric emptying (DGE), intra-abdominal abscess, hemorrhage, superficial surgical site infection (SSI), and readmission. Between groups 1.1 and 1.2, there was a significant difference in clinically relevant DGE (44.4% vs. 5.9%, p = 0.014) and Clavien–Dindo ≥ III complications (59.3% vs. 88.2%, p = 0.040). There were no significant differences in median overall survival and disease-free survival (DFS) between groups 1 and 2. Groups 1.1 and 1.2 had a significant difference in DFS (10 vs. 5 months, p = 0.017). In this group of patients, PBD was associated with increased bacterial colonization of the bile, without a significant increase in postoperative complications or influence in survival. ERCP seems to contribute to the development of clinically significant DGE. Patients undergoing PTC appear to have an early recurrence.
Performing the ABC Method Twice for Gastric Cancer Risk Stratification: A Retrospective Study Based on Data from a Large-Scale Screening Facility
Authors: Mizutani Satoru , Takahashi Yu , Shimamoto Takeshi , Nakagawa Hideki , Hisada Hiroyuki , Oshio Kaori , Kubota Dai , Mizutani Hiroya , Ohki Daisuke , Sakaguchi Yoshiki , Yakabi Seiichi , Niimi Keiko , Kakushima Naomi , Tsuji Yosuke , Wada Ryoichi , Yamamichi Nobutake , Fujishiro Mitsuhiro
Published: March 2023

The ABC method is a classification method used for stratifying the risk of gastric cancer. However, whether the ABC method should be performed only once or multiple times throughout an individual’s lifetime remains unclear. Therefore, this study aimed to analyze whether performing ABC screening twice in a lifetime is useful. We retrospectively analyzed the data of individuals who participated in health checkups in 2010 and 2015. We collected data on patient characteristics, pepsinogen levels, anti-Helicobacter pylori antibody titers, and the presence of gastric cancer. Overall, 7129 participants without a history of H. pylori eradication were included in this study. The participants’ average age in 2010 was 48.4 ± 8.3 years, and 58.1% were male. In addition, 11 and 20 cases of new H. pylori infection (0.15%) and spontaneous eradication (0.28%), respectively, were recorded. No significant difference was found in the incidence of gastric cancer between participants who underwent the ABC method once and those who underwent it twice (Group A: 0.16% vs. 0.16%; Group B: 0.47% vs. 0.39%; and Group C + D: 1.97% vs. 1.82%). Therefore, performing the ABC method twice, 5 years apart, does not significantly improve gastric cancer risk stratification.
An Improved Skin Lesion Boundary Estimation for Enhanced-Intensity Images Using Hybrid Metaheuristics
Authors: Malik Shairyar , Akram Tallha , Awais Muhammad , Khan Muhammad Attique , Hadjouni Myriam , Elmannai Hela , Alasiry Areej , Marzougui Mehrez , Tariq Usman
Published: March 2023

The demand for the accurate and timely identification of melanoma as a major skin cancer type is increasing daily. Due to the advent of modern tools and computer vision techniques, it has become easier to perform analysis. Skin cancer classification and segmentation techniques require clear lesions segregated from the background for efficient results. Many studies resolve the matter partly. However, there exists plenty of room for new research in this field. Recently, many algorithms have been presented to preprocess skin lesions, aiding the segmentation algorithms to generate efficient outcomes. Nature-inspired algorithms and metaheuristics help to estimate the optimal parameter set in the search space. This research article proposes a hybrid metaheuristic preprocessor, BA-ABC, to improve the quality of images by enhancing their contrast and preserving the brightness. The statistical transformation function, which helps to improve the contrast, is based on a parameter set estimated through the proposed hybrid metaheuristic model for every image in the dataset. For experimentation purposes, we have utilised three publicly available datasets, ISIC-2016, 2017 and 2018. The efficacy of the presented model is validated through some state-of-the-art segmentation algorithms. The visual outcomes of the boundary estimation algorithms and performance matrix validate that the proposed model performs well. The proposed model improves the dice coefficient to 94.6% in the results.
Wilson’s Disease—Genetic Puzzles with Diagnostic Implications
Authors: Gromadzka Grażyna , Bendykowska Maria , Przybyłkowski Adam
Published: March 2023

(1) Introduction: Wilson’s disease (WND) is an autosomal recessive disorder of copper metabolism. The WND gene is ATP7B, located on chromosome 13. WND is characterized by high clinical variability, which causes diagnostic difficulties. (2) Methods: The PubMed, Science Direct, and Wiley Online Library medical databases were reviewed using the following phrases: “Wilson’s disease”, “ATP7B genotype”, “genotype-phenotype”, “epigenetics”, “genetic modifiers”, and their combinations. Publications presenting the results of experimental and clinical studies, as well as review papers, were selected, which concerned: (i) the diversity of genetic strategies and tests used in WND diagnosis; (ii) the difficulties of genetic diagnosis, including uncertainty as to the pathogenicity of variants; (iii) genetic counseling; (iv) phenotypic effects of ATP7B variants in patients with WND and in heterozygous carriers (HzcWND); (v) genetic and epigenetics factors modifying the clinical picture of the disease. (3) Results and conclusions: The genetic diagnosis of WND is carried out using a variety of strategies and tests. Due to the large number of known variants in the ATP7B gene (>900), the usefulness of genetic tests in routine diagnostics is still relatively small and even analyses performed using the most advanced technologies, including next-generation sequencing, require additional tests, including biochemical evidence of abnormal copper metabolism, to confirm the diagnosis of WND. Pseudodominant inheritance, the presence of three various pathogenic variants in the same patient, genotypes indicating the possibility of segmental uniparental disomy, have been reported. Genotype–phenotype relationships in WND are complex. The ATP7B genotype, to some extent, determines the clinical picture of the disease, but other genetic and epigenetic modifiers are also relevant.
Non-Laboratory-Based Risk Prediction Tools for Undiagnosed Pre-Diabetes: A Systematic Review
Authors: Cheng Will Ho-Gi , Mi Yuqi , Dong Weinan , Tse Emily Tsui-Yee , Wong Carlos King-Ho , Bedford Laura Elizabeth , Lam Cindy Lo-Kuen
Published: March 2023

Early detection of pre-diabetes (pre-DM) can prevent DM and related complications. This review examined studies on non-laboratory-based pre-DM risk prediction tools to identify important predictors and evaluate their performance. PubMed, Embase, MEDLINE, CINAHL were searched in February 2023. Studies that developed tools with: (1) pre-DM as a prediction outcome, (2) fasting/post-prandial blood glucose/HbA1c as outcome measures, and (3) non-laboratory predictors only were included. The studies’ quality was assessed using the CASP Clinical Prediction Rule Checklist. Data on pre-DM definitions, predictors, validation methods, performances of the tools were extracted for narrative synthesis. A total of 6398 titles were identified and screened. Twenty-four studies were included with satisfactory quality. Eight studies (33.3%) developed pre-DM risk tools and sixteen studies (66.7%) focused on pre-DM and DM risks. Age, family history of DM, diagnosed hypertension and obesity measured by BMI and/or WC were the most common non-laboratory predictors. Existing tools showed satisfactory internal discrimination (AUROC: 0.68–0.82), sensitivity (0.60–0.89), and specificity (0.50–0.74). Only twelve studies (50.0%) had validated their tools externally, with a variance in the external discrimination (AUROC: 0.31–0.79) and sensitivity (0.31–0.92). Most non-laboratory-based risk tools for pre-DM detection showed satisfactory performance in their study populations. The generalisability of these tools was unclear since most lacked external validation.
Optimal Position and Target Rate for Covert Communication in UAV-Assisted Uplink RSMA Systems
Authors: Duan Zhengxiang , Yang Xin , Zhang Tao , Wang Ling
Published: March 2023

With the explosive increase in demand for wireless communication, the issue of wireless communication security has also become a growing concern. In this paper, we investigate a novel covert communication for unmanned aerial vehicle (UAV)-assisted uplink rate-splitting multiple access (RSMA) systems, where a UAV adopts the rate-splitting (RS) strategy to increase the total transmission rate while avoiding deteriorating the covert transmission of a ground user. In the proposed system, a ground user and a UAV adopt the RSMA scheme to simultaneously communicate with a base station surveilled by an evil monitor. The UAV acts as both the transmitter and the friendly jammer to cover the ground user’s transmission with random power. To maximize the expected sum rate (ESR), we first study the RS strategy and obtain the optimal power allocation factor. Then, the closed-form of minimum detection error probability (DEP), ESR, and optimal target rate of the UAV are derived. Constrained by the minimum DEP and expected covert rate (ECR), we maximize the ESR by optimizing the position and target rate of the UAV. Numerical results show that the proposed scheme outperforms the traditional NOMA systems in terms of ESR with the same DEP and ECR.
Spectral-Spatial Attention Rotation-Invariant Classification Network for Airborne Hyperspectral Images
Authors: Shi Yuetian , Fu Bin , Wang Nan , Cheng Yinzhu , Fang Jie , Liu Xuebin , Zhang Geng
Published: March 2023

An airborne hyperspectral imaging system is typically equipped on an aircraft or unmanned aerial vehicle (UAV) to capture ground scenes from an overlooking perspective. Due to the rotation of the aircraft or UAV, the same region of land cover may be imaged from different viewing angles. While humans can accurately recognize the same objects from different viewing angles, classification methods based on spectral-spatial features for airborne hyperspectral images exhibit significant errors. The existing methods primarily involve incorporating image or feature rotation angles into the network to improve its accuracy in classifying rotated images. However, these methods introduce additional parameters that need to be manually determined, which may not be optimal for all applications. This paper presents a spectral-spatial attention rotation-invariant classification network for the airborne hyperspectral image to address this issue. The proposed method does not require the introduction of additional rotation angle parameters. There are three modules in the proposed framework: the band selection module, the local spatial feature enhancement module, and the lightweight feature enhancement module. The band selection module suppresses redundant spectral channels, while the local spatial feature enhancement module generates a multi-angle parallel feature encoding network to improve the discrimination of the center pixel. The multi-angle parallel feature encoding network also learns the position relationship between each pixel, thus maintaining rotation invariance. The lightweight feature enhancement module is the last layer of the framework, which enhances important features and suppresses insignificance features. At the same time, a dynamically weighted cross-entropy loss is utilized as the loss function. This loss function adjusts the model’s sensitivity for samples with different categories according to the output in the training epoch. The proposed method is evaluated on five airborne hyperspectral image datasets covering urban and agricultural regions. Compared with other state-of-the-art classification algorithms, the method achieves the best classification accuracy and is capable of effectively extracting rotation-invariant features for urban and rural areas.
Synchrony-Division Neural Multiplexing: An Encoding Model
Authors: Rezaei Mohammad R. , Saadati Fard Reza , Popovic Milos R. , Prescott Steven A. , Lankarany Milad
Published: March 2023

Cortical neurons receive mixed information from the collective spiking activities of primary sensory neurons in response to a sensory stimulus. A recent study demonstrated an abrupt increase or decrease in stimulus intensity and the stimulus intensity itself can be respectively represented by the synchronous and asynchronous spikes of S1 neurons in rats. This evidence capitalized on the ability of an ensemble of homogeneous neurons to multiplex, a coding strategy that was referred to as synchrony-division multiplexing (SDM). Although neural multiplexing can be conceived by distinct functions of individual neurons in a heterogeneous neural ensemble, the extent to which nearly identical neurons in a homogeneous neural ensemble encode multiple features of a mixed stimulus remains unknown. Here, we present a computational framework to provide a system-level understanding on how an ensemble of homogeneous neurons enable SDM. First, we simulate SDM with an ensemble of homogeneous conductance-based model neurons receiving a mixed stimulus comprising slow and fast features. Using feature-estimation techniques, we show that both features of the stimulus can be inferred from the generated spikes. Second, we utilize linear nonlinear (LNL) cascade models and calculate temporal filters and static nonlinearities of differentially synchronized spikes. We demonstrate that these filters and nonlinearities are distinct for synchronous and asynchronous spikes. Finally, we develop an augmented LNL cascade model as an encoding model for the SDM by combining individual LNLs calculated for each type of spike. The augmented LNL model reveals that a homogeneous neural ensemble model can perform two different functions, namely, temporal- and rate-coding, simultaneously.
Student–Teacher Role Reversal at University Level—An Experience in Naval Engineering Education
Authors: Díaz Ojeda Héctor Rubén , Pérez-Arribas Francisco , Pérez-Sánchez Julio
Published: March 2023

The European Higher Education Area encourages a substantial change in the roles that students and teachers play at university. Student participation in the learning process should be primarily active, while teachers should adopt a guiding and mediating position. This paper describes a learning experience where an evolution of the roles of the trainers and learners is proposed. This methodology was implemented in the 2021/2022 and 2022/2023 university courses on the Bachelor in Naval Engineering. Students taking these courses are enrolled in the last year out of four of their Bachelor’s and are given the task of changing their role from students to teachers by teaching a lesson. No previous knowledge about the lesson is required; therefore, this learning activity is a double challenge for the students, as they must, on the one hand, learn a new topic and, on the other hand, be able to explain the topic to their colleagues. Surveys related to the activity and the classmates’ performance were carried out once the activity was completed. The results of the surveys show that students acquire technical knowledge more easily than traditional class and strengthen different skills, such as their self-esteem and communication ability. Additionally, the activity indicates the importance and necessity of boosting their autonomous work capacity, since they will be confronted with similar duties in their professional career. Finally, the proposed activity also reduces students’ boredom in subjects that they are initially uninterested in.