bluuebunny/crossref_metadata_2025 huggingface dataset:
Toward objective monitoring of Parkinson's disease motor symptoms using a wearable device: wearability and performance evaluation of PDMonitor®
Authors: Antonini Angelo , Reichmann Heinz , Gentile Giovanni , Garon Michela , Tedesco Chiara , Frank Anika , Falkenburger Bjoern , Konitsiotis Spyridon , Tsamis Konstantinos , Rigas Georgios , Kostikis Nicholas , Ntanis Adamantios , Pattichis Constantinos
Published: May 2023

Parkinson's disease (PD) is characterized by a variety of motor and non-motor symptoms. As disease progresses, fluctuations in the response to levodopa treatment may develop, along with emergence of freezing of gait (FoG) and levodopa induced dyskinesia (LiD). The optimal management of the motor symptoms and their complications, depends, principally, on the consistent detection of their course, leading to improved treatment decisions. During the last few years, wearable devices have started to be used in the clinical practice for monitoring patients' PD-related motor symptoms, during their daily activities. This work describes the results of 2 multi-site clinical studies (PDNST001 and PDNST002) designed to validate the performance and the wearability of a new wearable monitoring device, the PDMonitor®, in the detection of PD-related motor symptoms. For the studies, 65 patients with Parkinson's disease and 28 healthy individuals (controls) were recruited. Specifically, during the Phase I of the first study, participants used the monitoring device for 2–6 h in a clinic while neurologists assessed the exhibited parkinsonian symptoms every half hour using the Unified Parkinson's Disease Rating Scale (UPDRS) Part III, as well as the Abnormal Involuntary Movement Scale (AIMS) for dyskinesia severity assessment. The goal of Phase I was data gathering. On the other hand, during the Phase II of the first study, as well as during the second study (PDNST002), day-to-day variability was evaluated, with patients in the former and with control subjects in the latter. In both cases, the device was used for a number of days, with the subjects being unsupervised and free to perform any kind of daily activities. The monitoring device produced estimations of the severity of the majority of PD-related motor symptoms and their fluctuations. Statistical analysis demonstrated that the accuracy in the detection of symptoms and the correlation between their severity and the expert evaluations were high. As a result, the studies confirmed the effectiveness of the system as a continuous telemonitoring solution, easy to be used to facilitate decision-making for the treatment of patients with Parkinson's disease.
Case report: A choroidal fissure pial arteriovenous malformation inducing venous congestive edema of the medulla oblongata and cervicothoracic spinal cord presented with proximal arm predominant weakness
Authors: Jiang Yun , Zhou Ying , Yang Ximeng , Sheng Aizhen , Lu Jun
Published: May 2023

Intracranial dural arteriovenous fistula (DAVF) can induce remote myelopathy via spinal perimedullary venous drainage. In the present study, we report a rare case of intracranial pial arteriovenous malformation (AVM)-related myelopathy. A 52-year-old man presented with progressive, predominantly proximal weakness and muscle atrophy in bilateral upper limbs, urinary retention, and hyperreflexia in bilateral upper and lower limbs. Brain and cervicothoracic MRI showed longitudinal myelopathy extending from the medulla oblongata to the T6 level, with perimedullary enlarged veins from the C1 to T12 level, and remarkable enhancement in bilateral anterior horns from the C2 to C7 level. Cerebral angiography revealed a choroidal fissure AVM, which was supplied by the left anterior choroidal artery and drained exclusively by an inferior ventricular vein descending toward the spinal perimedullary veins. After endovascular embolization of the feeding pedicle, nidus, and proximal segment of the draining vein, the patient's neurological deficits rapidly improved, and a significant recovery was achieved 3 months after the procedure. This rare case indicates that intracranial pial AVM can also cause extensive congestive myelopathy with similar mechanisms underlying intracranial and craniocervical DAVF cases, and gray matter in the spinal cord might be more susceptible to ischemia induced by intraspinal venous hypertension.
Comparative analysis of outcomes following craniotomy and expanded endoscopic endonasal approach resection of tuberculum sellae meningiomas: a single-institution study
Authors: Feng Zhenguang , Li Chuzhong , Cao Lei , Liu Chunhui , Qiao Ning , Wu Wentao , Ma Guofo , Gui Songbai
Published: May 2023

Background

Traditionally, supratentorial craniotomy has been used to sever tuberculum sellae meningiomas (TSMs), but there has been a remarkably increasing tendency of extended endoscopic endonasal approach (EEEA) used to treat TSMs in the recent decade. Several documents have described the advantages and disadvantages of both approaches, but there is no consensus on whether one is superior to the other.

Objective

This study aimed to compare surgical outcomes between craniotomy and EEEA for TSMS treated at our institution.

Methods

From January 2015 to December 2021, a total of 84 cases of TSMs were included in this study. Cases were separated into two groups: the craniotomy group and the EEEA group. Their anamneses and surgical records were reviewed. Demographic data, presenting symptoms, tumor volume, extent of resection, visual outcomes, and follow-up data were tabulated. The Kaplan–Meier curves were constructed for the PFS for both cohorts.

Results

Complete data were available for 84 surgeries; 39 cases were treated via craniotomy, and 45 were treated via EEEA. Patient demographic data, pre-operative symptoms, and tumor characteristics were similar between the two cohorts. The extent of resection was similar between the two groups (GTR: 91.11% EEEA vs. 87.18% craniotomy; STR 8.89 vs. 12.82%, p = 0.91). There was no difference in visual outcomes between both groups (92.1 vs. 84.84%, p = 0.46). An increased rate of cranial nerve injury was noted in the craniotomy group (0 vs. 10.25%, p = 0.04). Post-operative CSF leak rate occurred in one patient in the EEEA group. The PFS curves (p = 0.52) and recurrence/progression rates (13.33 vs. 20.51%, p = 0.39) were similar between the two groups.

Conclusion

Both EEEA and craniotomy can successfully sever TSMs. The recurrence/progression rate and PFS appear to be similar between the two groups. Although there are no differences in EOR and visual outcomes between the two groups, there was a clear trend in the EEEA group to obtain a better outcome. CSF leakage was common in the EEEA cohort, whereas the rate of cranial nerve injury was found to be higher in the craniotomy cohort. We believe that our data support the conclusion that EEEA surgery is the preferred approach for the removal of TSMs.
Capsular warning syndrome and its clinical awareness and therapeutic approach: two case reports and a systematic review of the literature
Authors: Martínez Hector R. , Figueroa-Sanchez Jose A. , Arreola-Aldape Carlos A. , Moran Guerrero Jose Alberto , Trujillo-Bracho Ana Luisa , Cantú López Alejandro
Published: May 2023

Introduction

Capsular warning syndrome (CWS) is characterized by recurrent stereotyped episodes of unilateral transient motor and/or sensory symptoms affecting the face and upper and lower limbs, without cortical signs in 24 h and with a high risk of developing stroke. Among the possible underlying mechanisms, small perforating artery disease is the most common. The aim was to assess the most common risk factors, the therapeutic alternatives, and the different outcomes in patients with CWS, along with the presentation of two cases treated in our Emergency Department.

Methods

Stroke Code, launched at our institution in January 2017, was triggered 400 times, and by December 2022, 312 patients were admitted as having an acute ischemic stroke. Among them, two of them fulfilled the criteria of CWS. A systematic search was carried out in PubMed, Scopus, and Web of Science databases to seek demography and therapeutic approaches in CWS.

Results

Of 312 cases, two with acute ischemic stroke exhibited CWS. The first patient had six events of right hemiparesis with recovery in 10–30 min; after MRI and digital subtraction angiography (DSA), he received apixaban and clopidogrel; however, a day after admission, he developed ischemic infarction with partial recovery. The second patient presented five transient events of right hemiparesis. After MRI and DSA with an intra-arterial infusion of nimodipine, oral aspirin, and ticagrelor, he presented another event-developing stroke and was discharged with partial recovery. A systematic review found 190 cases of CWS in 39 articles from 1993 to 2022. Most were male subjects (66.4%), and hypertension (60%), smoking (36%), diabetes (18%), and dyslipidemia (55%) were the most common risk factors. Over 50% of the cases were secondary to small perforating artery disease. The most commonly used treatments were dual antiplatelet therapy (DAT), recombinant tissue plasminogen activator, and anticoagulant therapy (ACT), where the combination of DAT plus ACT was linked to the most positive functional outcomes (82.6%).

Conclusion

Our cases fit with the description of patients with partial recovery and risk factors (hypertension, diabetes, and smoking) in male patients. There is a lack of evidence regarding the best treatment option; dual antiplatelet therapy and anticoagulation therapy are strong contenders for a favorable result.
New advancements in the management of Neuromyelitis Optica spectrum disease: literature review
Authors: Sudhakar Padmaja , Abusamra Khawla , Thandampallayam Mangayarkarasi , Kini Ashwini
Published: May 2023

Neuromyelitis Optica spectrum disorder (NMOSD) is a relapsing autoimmune disease of the central nervous system (CNS) where aquaporin-4 water channels are the antigenic target of the disease. The spectrum of the disease involves regions of the CNS where the water channel is widely expressed including the spinal cord, the optic nerve, dorsal medulla, brainstem, and thalamus/hypothalamus. Management of NMOSD includes acute as well as long term treatment. Acute symptoms are typically treated with intravenous corticosteroids and/or plasma exchange while long-term treatment involves the use of immunosuppression/immune modulation. The year 2019 is thought to be the “year of the NMOSD” as three new medications became available for this devastating disease. In this review, FDA approved NMOSD medications are discussed.
Isoperistaltic gastric tube for long gap esophageal atresia (LGEA) in newborn, infants, and toddlers: a case-control study from a tertiary center
Authors: Zarfati Angelo , Tambucci Renato , Bagolan Pietro , Conforti Andrea
Published: May 2023

Background

Limited evidence exists about outcomes after gastric tube formation as “rescue” technique to avoid esophageal replacement in long gap esophageal atresia (LGEA). The last ERNICA Consensus Conference on the Management of LGEA has placed the techniques of gastric tubulization among the priorities for future research.

Aims

Evaluate personal experience with Isoperistaltic Gastric Tube (IGT) and compare its outcomes with other more popular techniques for LGEA.

Methods

A case-control study has been conducted. A retrospective monocentric analysis of LGEA patients (period: 2010–19) has been conducted in all consecutive IGT patients and each of these has been type matched with two cases of LGEA treated with other techniques. The follow-up (FU) considered was 24-months.

Results

IGT and controls showed no statistically significant differences regarding preoperative variables like sex, gestational age, birth weight, syndromes, and EA type. However, IGT patients had a significantly longer esophageal GAP under boost pressure (4.5 vertebral bodies vs. 3.6, p = 0.019) at time of surgery. The analysis showed no statistical difference among the two groups about perioperative outcomes, ICU, or overall postoperative stay. No differences have been shown between IGT and controls during the follow-up regarding GERD, esophagitis, fundoplication, dysphagia, vocal cord paralysis, stenosis, and dilatations, auxologic data, need for anastomosis revision, oral aversion, and death.

Conclusions

Isoperistaltic Gastric Tube is safe and effective even in LGEA patients with longer gaps, with good perioperative, post-operative and middle-term outcomes. This procedure may be considered as an alternative to avoid esophageal substitution when a primary anastomosis seems impossible for a residual gap after traction and growth techniques.
Effect of tanshinone IIA for myocardial ischemia/reperfusion injury in animal model: preclinical evidence and possible mechanisms
Authors: Zhu Peng-Chong , Shen Jiayi , Qian Ren-Yi , Xu Jian , Liu Chong , Hu Wu-Ming , Zhang Ying , Lv Ling-Chun
Published: May 2023

Introduction: Tanshinone IIA (Tan IIA), the major active lipophilic ingredient of Radix Salviae Miltiorrhizae, exerts various therapeutic effects on the cardiovascular system. We aimed to identify the preclinical evidence and possible mechanisms of Tan IIA as a cardioprotective agent in the treatment of myocardial ischemia/reperfusion injury.

Methods: The study quality scores of twenty-eight eligible studies and data analyses were separately assessed using the CAMARADES 10-item checklist and Rev-Man 5.3 software.

Results: The study quality score ranged from 3/10 to 7/10 points. The present study provided preliminary preclinical evidence that Tan IIA could significantly decrease the myocardial infarct size, cardiac enzyme activity and troponin levels compared with those in the control group (p < 0.05).

Discussion: Tan IIA alleviated myocardial I/R injury via antioxidant, anti-inflammatory, anti-apoptosis mechanisms and improved circulation and energy metabolism. Thus, Tan IIA is a promising cardioprotective agent for the treatment of myocardial ischemia/reperfusion injury and should be further investigated in clinical trials.
Search for η-mesic nuclei: a review of experimental and theoretical advances
Authors: Khreptak Aleksander , Skurzok Magdalena , Moskal Paweł
Published: May 2023

In the mid-1980s, theoreticians predicted possible exotic states of an eta (η) meson and a nucleus bound by the strong interaction, so-called mesic nucleus. This led to extensive experimental efforts aimed at discovering such unstable states as well as understanding the underlying interactions via η meson production reactions. As experiments yielded new data, more and more sophisticated theoretical models were developed to understand the fundamental η-nucleon and η-nucleus interactions. After more than 35 years of research in this field, several signals have been identified as possible indications of hypothetical η-mesic nuclei, but there is still no general agreement or clear confirmation of their existence. In the paper, we review the study of the η-mesic nuclei with an emphasis on recent research activities.
Effect of pregnancy on female gait characteristics: a pilot study based on portable gait analyzer and induced acceleration analysis
Authors: Li Xin , Lu Zhenghui , Cen Xuanzhen , Zhou Yizheng , Xuan Rongrong , Sun Dong , Gu Yaodong
Published: May 2023

Introduction:The changes in physical shape and center of mass during pregnancy may increase the risk of falls. However, there were few studies on the effects of maternal muscles on gait characteristics and no studies have attempted to investigate changes in induced acceleration during pregnancy. Further research in this area may help to reveal the causes of gait changes in women during pregnancy and provide ideas for the design of footwear and clothing for pregnant women. The purpose of this study is to compare gait characteristics and induced accelerations between non-pregnant and pregnant women using OpenSim musculoskeletal modeling techniques, and to analyze their impact on pregnancy gait.

Methods:Forty healthy participants participated in this study, including 20 healthy non-pregnant and 20 pregnant women (32.25 ± 5.36 weeks). The portable gait analyzer was used to collect participants’ conventional gait parameters. The adjusted OpenSim personalized musculoskeletal model analyzed the participants’ kinematics, kinetics, and induced acceleration. Independent sample T-test and one-dimensional parameter statistical mapping analysis were used to compare the differences in gait characteristics between pregnant and non-pregnant women.

Results:Compared to the control group, pregnancy had a 0.34 m reduction in mean walking speed (p< 0.01), a decrease in mean stride length of 0.19 m (p< 0.01), a decrease in mean stride frequency of 19.06 step/min (p< 0.01), a decrease in mean thigh acceleration of 0.14 m/s2(p< 0.01), a decrease in mean swing work of 0.23 g (p< 0.01), and a decrease in mean leg falling strength of 0.84 g (p< 0.01). Induced acceleration analysis showed that pregnancy muscle-induced acceleration decreased in late pregnancy (p< 0.01), and the contribution of the gastrocnemius muscle to the hip and joint increased (p< 0.01).

Discussion:Compared with non-pregnant women, the gait characteristics, movement amplitude, and joint moment of pregnant women changed significantly. This study observed for the first time that the pregnant women relied more on gluteus than quadriceps to extend their knee joints during walking compared with the control group. This change may be due to an adaptive change in body shape and mass during pregnancy.
An efficient transformation method for genome editing of elite bread wheat cultivars
Authors: Biswal Akshaya K. , Hernandez L. Ruben B. , Castillo Ana I. R. , Debernardi Juan M. , Dhugga Kanwarpal S.
Published: May 2023

An efficient genetic transformation protocol is necessary to edit genes for trait improvement directly in elite bread wheat cultivars. We used a protein fusion between a wheat growth-regulating factor 4 (GRF4) and its interacting factor (GIF1) to develop a reproducible genetic transformation and regeneration protocol, which we then used to successfully transform elite bread wheat cultivars Baj, Kachu, Morocco, Reedling, RL6077, and Sujata in addition to the experimental cultivar Fielder. Immature embryos were transformed with the vector using particle bombardment method. Transformation frequency increased nearly 60-fold with the GRF4-GIF1-containing vectors as compared to the control vector and ranged from ~5% in the cultivar Kachu to 13% in the cultivar RL6077. We then edited two genes that confer resistance against leaf rust and powdery mildew directly in the aforementioned elite cultivars. A wheat promoter, TaU3 or TaU6, to drive the expression of guide RNA was effective in gene editing whereas the OsU3 promoter failed to generate any edits. Editing efficiency was nearly perfect with the wheat promoters. Our protocol has made it possible to edit genes directly in elite wheat cultivars and would be useful for gene editing in other wheat varieties, which have been recalcitrant to transformation thus far.
Syrians of today, Germans of tomorrow: the effect of initial placement on the political interest of Syrian refugees in Germany
Authors: Kassam Kamal , Becker Maria
Published: May 2023

Syrian nationals are not only the largest refugee group in Germany but also the third largest group of foreigners living in Germany. The naturalization trend among this group has been very pronounced in the last two years and is expected to increase sharply in the coming years. However, little is known about their political interest in German politics.1 Given the importance of “political interest” as an indicator of social integration and future active citizenship, this paper examines the extent to which Syrian refugees are interested in German politics and how local conditions at the time of arrival influence refugees' interest in German politics. We focus on three dimensions of the neighborhood context theory (social networks, economic situation, and political environment) in combination with traditional political participation theory. The empirical strategy relies on the exogenous allocation of refugees across federal states, which can be used to identify the effect of local characteristics on refugees' political interest. We use in our analysis a nationally representative sample in Germany (IAB-BAMF-SOEP-Refugee-Sample). Our findings suggest that ethnic social networks play a significant role in boosting newly arrived refugees' interest in German politics. Moreover, a higher unemployment rate among the foreign population is associated with an increase in political interest among Syrian refugees. We also confirm that a high political interest among the native population in Germany leads to a higher political interest among Syrian refugees. These results show that more attention needs to be paid to the integration of Syrian refugees and underline the need to reassess the efficiency of the distribution policy for Syrian refugees.
Evaluation of a short instrument for measuring health-related quality of life in oncological patients in routine care (HELP-6): an observational study
Authors: Schrage Theresa , Görlach Mirja , Betz Christian Stephan , Bokemeyer Carsten , Kröger Nicolaus , Mueller Volkmar , Krüll Andreas , Schulz Holger , Bleich Christiane
Published: May 2023

Purpose

Patient-reported outcomes have not been sufficiently implemented into the routine care of cancer patients because the existing instruments are often too long and complex or not cancer-specific. The aim of this study is the determination of psychometric properties and item reduction of a newly developed health-related quality of life (HrQoL) questionnaire for use in oncological clinical routines.

Methods

This observational study with a repeated measurements design included oncological inpatients and outpatients. A total of 630 patients participated at the first point of measurement and 404 at the second point of measurement. To evaluate the instrument, we conducted hierarchical confirmative factor analyses and for further validation correlated the resulting factors with standardized and validated HrQoL measurements. Test–retest reliability and responsiveness to change were tested.

Results

The developed questionnaire “HELP-6” (“Hamburg Inventory for Measuring Quality of Life in Oncological Patients”) has a six-factor structure and has moderate-to-good convergent validity (r= −0.25 –−0.68). Test–retest reliability was moderate-to-good (r =0.56−0.81, p < 0.001). Indications for responsiveness to change were found for three dimensions. The final version of the questionnaire HELP-6 has six dimensions with one item each.

Conclusion

With the HELP-6 instrument for measuring HrQoL in cancer patients, we provide a short and practical patient-reported outcome instrument. Though responsiveness to change could not be confirmed for all dimensions in this study, the HELP-6 includes time-efficient completion and evaluation and is informative in relevant HrQoL dimensions of cancer patients. Therefore, the HELP-6 poses an important addition to inpatient and outpatient routine cancer care.

Trial registration

This study was registered at Open Science Framework (https://osf.io/y7xce/), on 9 June 2018.
Long-term trends of HIV/AIDS incidence in India: an application of joinpoint and age–period–cohort analyses: a gendered perspective
Authors: Shri Neha , Bhattacharyya Krittika , Dhamnetiya Deepak , Singh Mayank , Jha Ravi Prakash , Patel Priyanka
Published: May 2023

Background

Monitoring the transmission patterns of human immunodeficiency virus (HIV) in a population is fundamental for identifying the key population and designing prevention interventions. In the present study, we aimed to estimate the gender disparities in HIV incidence and the age, period, and cohort effects on the incidence of HIV in India for identifying the predictors that might have led to changes in the last three decades.

Data and methods

This study utilizes data from the Global Burden of Disease Study for the period 1990–2019. The joinpoint regression analysis was employed to identify the magnitude of the changes in age-standardized incidence rates (ASIRs) of HIV. The average annual percentage changes in the incidence were computed, and the age–period–cohort analysis was performed.

Results

A decreasing trend in the overall estimates of age-standardized HIV incidence rates were observed in the period 1990–2019. The joinpoint regression analysis showed that the age-standardized incidence significantly declined from its peak in 1997 to 2019 (38.0 and 27.6 among males and females per 100,000 in 1997 to 5.4 and 4.6, respectively, in 2019). The APC was estimated to be 2.12 among males and 1.24 among females for the period 1990–2019. In recent years, although the gender gap in HIV incidence has reduced, females were observed to bear a proportionately higher burden of HIV incidence. Age effect showed a decline in HIV incidence by 91.1 and 70.1% among males and females aged between 15–19 years and 75–79 years. During the entire period from 1990–1994 to 2015–2019, the RR of HIV incidence decreased by 36.2 and 33.7% among males and females, respectively.

Conclusion

India is experiencing a decline in new HIV infections in recent years. However, the decline is steeper for males than for females. Findings highlight the necessity of providing older women and young women at risk with effective HIV prevention. This study emphasizes the need for large-scale HIV primary prevention efforts for teenage girls and young women.
A daily-level, within-person examination of emotion regulation as a mediator of the relationship between sleep and behavior in youth
Authors: Feghali Paulina T. , Iwamoto Brooke K. , Triplett Olivia M. , Rockwood Nicholas J. , Nelson Timothy D. , Van Dyk Tori R.
Published: May 2023

Objective

Youth who experience behavioral and emotional problems are at risk for sleep disturbance, while sleep disturbance also perpetuates behavioral and emotional problems. While the relationship between sleep and psychopathology in clinical mental health samples is well-established, exploration of the underlying mechanisms maintaining this relationship is limited. The purpose of this study is to explore within-person variability in emotion regulation as a mechanism of the relationship between sleep and psychopathology in a clinical youth sample.

Methods

Using a within-person design, 25 children (ages 6–11; 64% male; 44% non-Hispanic White) presenting to outpatient behavioral health treatment with mental health concerns were recruited to participate in a 14-day study. Daily reports of objective sleep duration via actigraphy, self-reported subjective sleepiness, and parent-reported internalizing and externalizing problems and emotion regulation were collected. Multilevel mediation analyses were used to examine the mediating effect of emotion regulation on the daily-level relationship between sleep and behavior problems.

Results

At the within-person level, emotion dysregulation was a significant mediator of the relationships between objective sleep duration and both externalizing [MCCI (0.0005–0.0063)] and internalizing problems [MCCI (0.0001–0.0025)]. Contrary to hypotheses, when youth slept more than usual, internalizing and externalizing problems were worse through the indirect effect of increased emotion dysregulation.

Conclusions

Inconsistencies in schedules and routines, even if in a positive direction, may have short-term negative consequences for youth with emotional and behavioral concerns. Future research should look to address sleep variability and how deviations in routine may impact behavior more broadly, through the indirect effects of emotion regulation.
Pathogenicity virulence of Beauveria spp. and biosafety of the BbMQ strain on adult ectoparasitic beetles, Dastarcus helophoroides Fairmaire (Coleoptera: Colydiidae)
Authors: Zhou Youjun , Lu Ciding , Chen Zhenghao , Ye Shuting , Fang Xinyuan , Zhang Zhuhe , Cai Shouping , Zhang Feiping , Liang Guanghong
Published: May 2023

Introduction

Beauveria spp. and Dastarcus helophoroides Fairmaire adults were simultaneously released to attack elder larvae or pupae of Monochamus alternatus in pine forests in China. However, little is known about the pathogenicity virulence and biosafety of Beauveria spp. on beneficial adults of D. helophoroides, and specific Beauveria bassiana (Bb) strains should be selected for synthetic release together with D. helophoroides.

Methods

A total of 17 strains of Beauveria spp. were collected, isolated, and purified, and then their mortality, cadaver rate, LT50, spore production, spore germination rate, and growth rate of D. helophoroide adults were calculated based on 0–20 days data after spore suspension and powder contact.

Results and discussion

The lethality rate of BbMQ, BbFD, and BbMH-03 strains to D. helophoroides exceeded 50%, and the cadaver rate reached 70.6%, among which the mortality rate (82.22%), cadaver rate (47.78%), spore production (1.32 × 109 spores/ml), spore germination rate (94.71%), colony dimension (49.15 mm2), and LT50 (10.62 d) of the BbMQ strain were significantly higher than those of other strains (P < 0.01), and the mortality of D. helophoroides adults increased significantly with increased spore suspension concentration, with the highest mortality reaching 92.22%. This strain was identified as Beauveria bassiana by morphological and molecular methods, while the BbWYS strain had a minimum lethality of only 5.56%, which was safer compared to other strains of adult D. helophoroide. Consequently, the biological characteristics and pathogenicity of different Beauveria bassiana strains varied significantly in their effects on D. helophoroide adults, and the safety of different strains should be assessed when they are released or sprayed to control multiple pests in the forest. The BbMQ strain should not be simultaneously sprayed with releasing D. helophoroide adults in the same forest, while the BbWYS strain can be used in concert with D. helophoroide to synergize their effect.
Accelerated high frequency rTMS induces time-dependent dopaminergic alterations: a DaTSCAN brain imaging study in healthy beagle dogs
Authors: Xu Yangfeng , Peremans Kathelijne , Salden Sofie , Audenaert Kurt , Dobbeleir Andre , Van Eeckhaut Ann , De Bundel Dimitri , Saunders Jimmy H. , Baeken Chris
Published: May 2023

Aim

The neurobiological effects of repetitive transcranial magnetic stimulation are believed to run in part through the dopaminergic system. Accelerated high frequency rTMS (aHF-rTMS), a new form of stimuli delivery, is currently being tested for its usefulness in treating human and canine mental disorders. However, the short-and long-term neurobiological effects are still unclear, including the effects on the dopaminergic system. In aHF-rTMS, multiple sessions are delivered within 1 day instead of one session per day, not only to accelerate the time to response but also to increase clinical efficacy. To gain more insight into the neurobiology of aHF-rTMS, we investigated whether applying five sessions in 1 day has direct and/or delayed effects on the dopamine transporter (DAT), and on dopamine metabolites of cerebrospinal fluid (CSF) in beagles.

Materials and methods

Thirteen beagles were randomly divided into two groups: five active stimulation sessions (n = 9), and 5 sham stimulation sessions (n = 4). Using DaTSCAN, DAT binding indices (BI) were obtained at baseline, after 1 day, 1 month, and 3 months post stimulation. CSF samples were collected after each scan.

Results

Active aHF-rTMS significantly reduced striatal DAT BI 1 day post-active stimulation session (p < 0.01), and the effect lasted to 1 month (p < 0.01). No significant DAT BI change was found in sham group. No significant changes in dopamine metabolites of CSF were found.

Conclusion

Although no significant effects on CSF dopamine metabolites were observed, five sessions of active aHF-rTMS significantly decreased striatal DAT BI after 1 day and up to 1 month post stimulation, indicating immediate and delayed effects on the brain dopaminergic system. Our findings in healthy beagles further substantiate the assumption that (a)HF-rTMS affects the brain dopaminergic system and it may pave the way to apply (a)HF-rTMS treatment in behaviorally disturbed dogs.
Classification of Systems and Maintenance Models
Authors: Ulansky Vladimir , Raza Ahmed
Published: May 2023

Maintenance is an essential part of long-term overall equipment effectiveness. Therefore, it is essential to evaluate maintenance policies’ effectiveness in addition to planning them. This study provides a classification of technical systems for selecting maintenance effectiveness indicators and a classification of maintenance models for calculating these indicators. We classified the systems according to signs, such as system maintainability, failure consequences, economic assessment of the failure consequences, and temporary mode of system use. The classification of systems makes it possible to identify 13 subgroups of systems with different indicators of maintenance effectiveness, such as achieved availability, inherent availability, and average maintenance costs per unit of time. When classifying maintenance models, we used signs such as the system structure in terms of reliability, type of inspection, degree of unit restoration, and external manifestations of failure. We identified one hundred and sixty-eight subgroups of maintenance models that differed in their values for specified signs. To illustrate the proposed classification of maintenance models, we derived mathematical equations to calculate all considered effectiveness indicators for one subgroup of models related to condition-based maintenance. Mathematical models have been developed for the case of arbitrary time-to-failure law and imperfect inspection. We show that the use of condition-based maintenance significantly increases availability and reduces the number of inspections by more than half compared with corrective maintenance.
A Simulation Framework for Aircraft Take-Off Considering Ground Effect Aerodynamics in Conceptual Design
Authors: Abu Salem Karim , Palaia Giuseppe , Chiarelli Mario R. , Bianchi Mario
Published: May 2023

The development of novel aircraft concepts and propulsion technologies requires up-to-date physics-based methods and tools for conceptual aircraft design. In this context, a simulation model for the take-off manoeuvre is proposed in this article, to be employed in the conceptual design phase for aircraft whether of traditional or innovative configuration. The model is capable of evaluating the longitudinal dynamics, both translational and rotational, of the aircraft considered as a rigid body, and influenced by the aerodynamic effects introduced by the presence of the ground. The ground effect, indeed, induces variations in the aerodynamic forces depending on the distance and the attitude of the lifting surfaces from the ground, which may significantly influence the aeromechanical characteristics of the aircraft during the evolution of the take-off manoeuvre. The simulation model is based on the numerical solution of the equations of the dynamics of the rigid aircraft in the longitudinal plane and integrates a vortex lattice aerodynamic solver to evaluate the aerodynamic and aeromechanical characteristics of the aircraft considering the ground effect in each time-step. The proposed approach is configuration independent, as it can model the geometry, evaluate the aerodynamics, and simulate the dynamics of aircraft with any lifting architecture; furthermore, the simulation model is fast and flexible, making it effective for the conceptual phase of aircraft design. The paper proposes the description of the take-off manoeuvre of two aircraft with different airframes: one with a conventional tube-and-wing architecture and one with a box-wing lifting system. The results proposed highlight the potential of the simulation model to detect aeromechanic and dynamic differences during the development of the manoeuvre for different aircraft configurations, and to assess the significance of considering ground effect aerodynamics.
External Factors Facilitating Quality Certification of Agricultural Products in China: Insights from Cooperatives in the Sichuan Province
Authors: Liu Min , Yang Jinxiu , Zheng Guoquan , Shang Ping , Li Yipei
Published: May 2023

Promoting quality certification of agricultural products is a matter of the sustainable development of agriculture and the transformation of the global agri-food system, and cooperatives are an important carrier to achieve the above policy goals. However, few scholars have included external resources and cooperative quality certification behavior in an analytical framework from the perspective of resource embedding. This study combines resource dependence theory and embeddedness theory, based on survey data from 230 agricultural cooperatives in six counties of Sichuan Province, China. This paper constructs a theoretical analysis framework of government resources, market resources and cooperative quality certification, and uses binary Logit regression model to empirically study the correlation between external resources and cooperative quality certification. The results show that: (1) Cooperatives do not receive a high proportion of external resource support, with 30% and 47% receiving support from the government and market respectively; the proportion of cooperatives carrying out product quality certification was relatively low, about 29.57%. (2) Government resources and market resources have a significant positive effect on improving the quality certification of cooperatives, and the promotion effect of the market is greater than that of the government. (3) Mechanism analysis shows that government resources promote quality certification in cooperatives mainly through regulations on agricultural inputs, while market resources can jointly play a role on regulations on agricultural inputs and market expectations for certified agricultural products.
Endophytic Effect of the South African Beauveria bassiana Strain PPRI 7598 on the Population Growth and Development of the Russian Wheat Aphid, Diuraphis noxia
Authors: Motholo Lisemelo Francina , Booyse Marde , Hatting Justin Louis , Tsilo Toi John , Lekhooa Makhotso , Thekisoe Oriel
Published: May 2023

The Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov), is one of the main pests of small-grain cereal crops, including bread wheat, Triticum aestivum (Poaceae). In a series of glasshouse experiments, we evaluated the systemic effect of endophytic Beauveria bassiana strain PPRI 7598 on D. noxia biotype RWASA1 using three South African wheat cultivars, Gariep, Molopo, and Kariega. The objectives of the study were: (a) to determine the effect of endophytism on RWA reproduction and population growth, and (b) to assess the effect of the endophyte on aphid biomass and T. aestivum response to D. noxia herbivory using a damage rating index. Plant endophytic colonisation was confirmed before each trial using a B. bassiana-selective medium. Three independent trials were performed 10 days apart under glasshouse conditions. The effect of the endophyte-treated versus non-treated plants varied significantly in terms of net reproductive rate (R0) and the intrinsic rate of increase (rm) of the D. noxia population. Overall, the endophyte significantly reduced D. noxia R0 by approximately 14 nymphs/female and decreased the aphid mass by 13% in treated plants, whereas the mean aphid mass increased by 17% in control plants in all pooled cultivars. These findings demonstrated the endophytic potential of B. bassiana strain PPRI 7598 for suppression of D. noxia populations in RWASA1-susceptible cultivars. The integration of B. bassiana endophytism with host plant resistance may counteract biotype development and support a more sustainable approach towards RWA control in integrated pest management programmes.
Evaluation of Alternative Doxycycline Antibiotic Regimes in an Inhalational Murine Model of Q Fever
Authors: Clay Kate A. , Hartley M. Gill , Whelan Adam O. , Bailey Mark S. , Norville Isobel H.
Published: May 2023

The timing of the initiation of antibiotic treatment has been shown to impact the clinical outcome of many bacterial infections, including Q fever. Delayed, suboptimal or incorrect antibiotic treatment has been shown to result in poor prognosis, resulting in the progression of acute disease to long-term chronic sequalae. Therefore, there is a requirement to identify an optimal, effective therapeutic regimen to treat acute Q fever. In the study, the efficacies of different doxycycline monohydrate regimens (pre-exposure prophylaxis, post-exposure prophylaxis or treatment at symptom onset or resolution) were evaluated in an inhalational murine model of Q fever. Different treatment lengths (7 or 14 days) were also evaluated. Clinical signs and weight loss were monitored during infection and mice were euthanized at different time points to characterize bacterial colonization in the lungs and the dissemination of bacteria to other tissues including the spleen, brain, testes, bone marrow and adipose. Post-exposure prophylaxis or doxycycline treatment starting at symptoms onset reduced clinical signs, and also delayed the systemic clearance of viable bacteria from key tissues. Effective clearance was dependent on the development of an adaptive immune response, but also driven by sufficient bacterial activity to maintain an active immune response. Pre-exposure prophylaxis or post-exposure treatment at the resolution of clinical signs did not improve outcomes. These are the first studies to experimentally evaluate different doxycycline treatment regimens for Q fever and illustrate the need to explore the efficacy of other novel antibiotics.
Effects of Deacetylase Inhibition on the Activation of the Antioxidant Response and Aerobic Metabolism in Cellular Models of Fanconi Anemia
Authors: Bertola Nadia , Regis Stefano , Bruno Silvia , Mazzarello Andrea Nicola , Serra Martina , Lupia Michela , Sabatini Federica , Corsolini Fabio , Ravera Silvia , Cappelli Enrico
Published: May 2023

Fanconi anemia (FA) is a rare genetic disease characterized by a dysfunctional DNA repair and an oxidative stress accumulation due to defective mitochondrial energy metabolism, not counteracted by endogenous antioxidant defenses, which appear down-expressed compared to the control. Since the antioxidant response lack could depend on the hypoacetylation of genes coding for detoxifying enzymes, we treated lymphoblasts and fibroblasts mutated for the FANC-A gene with some histone deacetylase inhibitors (HDACi), namely, valproic acid (VPA), beta-hydroxybutyrate (OHB), and EX527 (a Sirt1 inhibitor), under basal conditions and after hydrogen peroxide addition. The results show that VPA increased catalase and glutathione reductase expression and activity, corrected the metabolic defect, lowered lipid peroxidation, restored the mitochondrial fusion and fission balance, and improved mitomycin survival. In contrast, OHB, despite a slight increase in antioxidant enzyme expressions, exacerbated the metabolic defect, increasing oxidative stress production, probably because it also acts as an oxidative phosphorylation metabolite, while EX527 showed no effect. In conclusion, the data suggest that VPA could be a promising drug to modulate the gene expression in FA cells, confirming that the antioxidant response modulation plays a pivotal in FA pathogenesis as it acts on both oxidative stress levels and the mitochondrial metabolism and dynamics quality.
Danthron, an Anthraquinone Isolated from a Marine Fungus, Is a New Inhibitor of Angiogenesis Exhibiting Interesting Antitumor and Antioxidant Properties
Authors: Vidal Isabel , Torres-Vargas José Antonio , Sánchez José María , Trigal Mónica , García-Caballero Melissa , Medina Miguel Ángel , Quesada Ana R.
Published: May 2023

The role played by a sustained angiogenesis in cancer and other diseases stimulates the interest in the search for new antiangiogenic drugs. In this manuscript, we provide evidence that 1,8- dihydroxy-9,10-anthraquinone (danthron), isolated from the fermentation broth of the marine fungus Chromolaenicola sp. (HL-114-33-R04), is a new inhibitor of angiogenesis. The results obtained with the in vivo CAM assay indicate that danthron is a potent antiangiogenic compound. In vitro studies with human umbilical endothelial cells (HUVEC) reveal that this anthraquinone inhibits certain key functions of activated endothelial cells, including proliferation, proteolytic and invasive capabilities and tube formation. In vitro studies with human breast carcinoma MDA-MB231 and fibrosarcoma HT1080 cell lines suggest a moderate antitumor and antimetastatic activity of this compound. Antioxidant properties of danthron are evidenced by the observation that it reduces the intracellular reactive oxygen species production and increases the amount of intracellular sulfhydryl groups in endothelial and tumor cells. These results support a putative role of danthron as a new antiangiogenic drug with potential application in the treatment and angioprevention of cancer and other angiogenesis-dependent diseases.
Mechanical Incipient Fault Detection and Performance Analysis Using Adaptive Teager-VMD Method
Authors: Li Huipeng , Xu Bo , Zhou Fengxing , Huang Pu
Published: May 2023

For large rotating machinery with low speed and heavy load, the incipient fault characteristics of rolling bearings are particularly weak, making it difficult to identify them effectively by direct signal processing methods. To resolve this issue, we propose a novel approach to detecting incipient fault features that combines signal energy enhancement and signal decomposition. First, the structure of a conventional Teager algorithm is modified to further increase the energy of the micro-impact component and hence the impact amplitude. Then, a kind of composite chaotic mapping is constructed to extend the original fruit fly optimization algorithm (FOA) framework, improving the FOA’s randomness and search power. The effective intrinsic mode functions (IMFs) are determined by searching for the optimal combination values of the key parameters of the variational mode decomposition (VMD) with the improved chaotic FOA (ICFOA). The kurtosis index is then used to select the IMFs that are most relevant to the fault characteristics information. Finally, the sensitive components are analyzed to identify multiple early fault characteristics and determine detailed information about the faults. Moreover, the approach is evaluated by a simulation signal and a measured signal. The comprehensive evaluation indicates that the approach has clear advantages over other excellent methods in extracting the incipient fault feature information of the equipment and has great potential for application in engineering.