bluuebunny/crossref_metadata_2025 huggingface dataset:
Forecasting Stock Price PT. Telkom Using Hybrid Time Series Regression Linear– Autoregressive Integrated Moving Average Model
Authors: Ramadani Kartika , Wahyuningsih Sri , Hayati Memi Nor
Published: January 2022

The hybrid method is a method of combining two forecasting models. Hybrid method is used to improve forecasting accuracy. In this study, the Time Series Regression (TSR) linear model will be combined with the Autoregressive Integrated Moving Average (ARIMA) model. The TSR linear model is used to obtain the model and residual value, then the residual value of the TSR linear model will be modeled by the ARIMA model. This combination method will produce a hybrid TSR linear-ARIMA model. The case study in this research is stock closing price (daily) of PT. Telkom Indonesia Tbk. The stock closing price (daily) of PT. Telkom Indonesia Tbk in 2020 showed an decreasing and increasing trend pattern. The results of this study obtained the best model of hybrid TSR linear-ARIMA (2,1,1) with the proportion of data training and testing is 70:30. In the best model, the MAD value is 56.595, the MAPE value is 1.880%, and the RMSE value is 78.663. It is also found that the hybrid TSR linear-ARIMA model has a smaller error value than the TSR linear model. The results of forecasting the stock price of PT. Telkom Indonesia Tbk for the period 02 January 2021 to 29 January 2021 formed a decreasing trend pattern.
Publications of the U.S. Army Engineer Research and Development Center; Appendix F: FY21 (October 2020-September 2021)
Authors: Dolan James
Published: January 2022

Publications issued October 2020 through September 2021 by the U.S. Army Engineer Research and Development Center (ERDC) are listed. The publications are grouped according to the technical laboratories or technical program for which they were prepared. Procedures for obtaining ERDC reports are included in the Preface.
Implementasi Model Pembelajaran Sentra Bahan Alam untuk Meningkatkan Kreativitas Anak Usia 4-5 Tahun
Authors: Munar Asyiful , Hibana Hibana , Surahman Susilo
Published: January 2022

Anak usia dini memiliki berbagai kemampuan yang perlu adanya stimulasi, pada lembaga ini masih menerapkan model pembelajaran klasikal, dimana pada model klasika ini yang paling ditonjolkan guru sebagai peran utama dalam pembelajaran. Dalam rangka untuk memenuhi kebutuhan peningkatan kreativitas bagi anak, TKN Pembina Sawang ingin menerapkan model pembelajaran sentra bahan alam untuk menumbuhkan kerativitas yang dimiliki anak. Penelitian ini berupa penelitian lapangan (field research) dengan metode kualitatif. Hasil observasi yang dilakukan terhadap guru yang dilakukan tiga kali pertemuan pada awal pembelajaran sampai selesai dengan sentra bahan alam, peneliti mendapati bahawa pembelajran sentra bahan alam untuk meningkatkan kreativitas anak melalui bahan alam pelepah pisang sudah berkembang dengan sangat baik.  Hal ini terlihat pada anak umur 4-5 tahun sudah mampu mengkreasikan sendiri berbagai macam bentuk-bentuk melalui media pelepah pisang. Berdasarkan gejala yang terdapa dilapangan, saran dari peneliti: didalam pembuatan RPPH diharapkan untuk mencantumkan KD (standar kompetensi). Agar apa yang ingin dicapai dalam proses pembelajaran dapat dilaksanakan dengan baik. Diharapkan kepada guru dalam penerpan model pembelajaran sentra bahan alam ini untuk menggunakan bahan yang ada dialam yang udah dijumpai dan tidak asing bagi anak, bisa apa saja asalkan tidak membahayakan anak dan selalu dalam pengawasan guru serta sesuai dengan tahap perkembangan anak.
Pengembangan Media Giant APE Tema Alat Transportasi Berbasis Saintifik untuk Peningkatan Keterampilan Motorik Anak TK Kelompok B
Authors: Pratiwi Dewi , Santoso Santoso , Kanzunnudin Moh
Published: January 2022

Keterampilan motorik mempunyai manfaat untuk perkembangan yang lain yaitu: bagi perkembangan fisiologis, sosial emosional, dan kognitifnya. Penelitian ini dilatarbelakangi oleh belum maksimalnya perkembangan motorik anak TK kelompok B pada tema alat transportasi karena penggunaan media pembelajaran yang kurang menarik serta penerapan pendekatan saintifik yang belum sepenuhnya terlaksana. Tujuan penelitian ini adalah menganalisis kebutuhan dan pengembangan media pembelajaran materi alat transportasi  untuk meningkatkan keterampilan motorik anak TK kelompok B. Penelitian dan Pengembangan (RD) menggunakan metode Borg and Gall. Populasi  penelitian anak TK kelompok B di kabupaten Kudus. Sampel dalam penelitian ini adalah anak kelompok B TK Pertiwi Bacin, TK Aisyiah Bustanul Athfal V, dan TK Nurus Shofa Kudus yang semuanya berjumlah 60 anak. Teknik pengumpulan data menggunakan observasi, angket dan tes wawancara. Teknik analisis data dilakukan melalui triangulasi data. Hasil Validasi ahli media dan ahli materi diperoleh kriteria baik. Produk layak diujicobakan dengan revisi. Produk yang dihasilkan berupa media pembelajaran giant APE dengan petunjuk penggunaanya.
Influence of Sand Casting Waste as Substitutor of Quartz Sand in Mortar
Authors: Prasetya Fandi Angga , Faria Niswatun , Susanto Tri Eddy , Firdaus Rizqi Ni’amul , Zamzami Yaski Achmad
Published: January 2022

Sand casting waste has the potential to replace quartz sand in mortar manufacture because it contains high silica. This study uses sand casting waste from the steel industry in Gresik, Indonesia to observe how it affects the quality of the mortar. Initial characterization were carried out to determine the properties of the material, including; magnetic test which results are not attracted by magnets, moisture content test with a value of 0.328%, XRD test to determine the crystallinity content which results contain 99.52% Silica Quartz, and XRF test to determine the content of the compound in which results are 81.25% Silica dominant. Then observations were made by making mortar with the replacement of quartz sand by sand casting with variations of 0% wt, 25% wt, 50% wt, and 100% wt and then tested its compressive strength at 3 days, 7 days, and 28 days. Based on the research that has been done, the optimum result using sand casting is at 25% wt with a compressive strength of 251.15 kgf/cm2 at 28 days of age which is higher than the standard.
KEPASTIAN HUKUM KEPEMILIKAN RUMAH SUSUN OLEH ORANG ASING DALAM UNDANG-UNDANG CIPTA KERJA
Authors: Waluja Hana Djaja , Hartanto Andy , Waluja Herlin Djaja
Published: January 2022

Perkembangan Zaman tidak hanya membawa perubahan dalam berbagai aspek kehidupan, melainkan juga perubahan pada aturan-aturan perundang-undangan. Perubahan akan aturan perundang-undangan yang ada saat ini diharapkan dapat meningkatkan perekonomian melalui investasi baik dari dalam ataupun luar negeri disamping kondisi pandemi Covid-19. Lahirnya Undang-Undang Nomor 11 Tahun 2020 tentang Cipta Kerja (UU CipKer) pada Paragraf 3 tentang Satuan Rumah Susun untuk Orang Asing Pasal 143-145 membawa perluasan makna bagi kepemilikan Hak Milik atas Satuan Rumah Susun (HMSRS), Peralihan hak serta penjaminannya sangat luas pemaknaannya hingga timbul ketidakpastian hukum khusunya bagi WNI. Untuk itu perlu dibahas untuk diketahui perluasan makna dalam pemberian kepemilikan HMSRS, peralihan hak serta penjaminan yang diberikan kepada orang asing. Metode penelitian yang digunakan yaitu penelitian hukum normatif dengan pendekatan undang-undang dan konseptual. Penelitian ini mempunyai isu hukum yaitu akibat hukum diberikan hak milik dan peralihan atas HMSRS kepada orang asing. Hasil penelitian ini, yaitu bahwa dengan diberikannya HMSRS kepada Orang Asing, dapat mengurangi kesejahteraan rakyat dalam mendapatkan tempat tinggal. Kedua, Peralihan Hak atas Tanah dan penjaminan khususnya HMSRS  yang diberikan untuk dan antar Orang Asing dapat membawa ketidakpastian hukum, terlebih tidak diaturnya aturan mengenai jelasnya peralihan dan penjaminan yang dapat dilakukan.
Pengaruh Kendali Kecepatan Motor DC Pada Chopper Drive
Authors: Zulkifli Rafli Setiawan
Published: January 2022

Abstrak - Motor DC adalah motor yang mudah dalam pengaplikasiannya. namun dalam pengaplikasiannya, kecepatan motor DC seringkali terjadi penurunan yang diakibatkan oleh beban sehingga kecepatan motor DC tidak konstan. Karena kecepatan motor DC sering terjadi penurunan dan tidak konstan maka terdapat berbagai penelitian yang sudah dilakukan. Penelitian ini membahas pengaruh pengendalian kecepatan motor DC dengan menggabungkan elektronika daya sebagai pencatu, sistem elektris pada motor DC, dan sistem mekanis dari beban motor DC yang disimulasikan menggunakan software Matlab. Penelitian ini menggunakan permodelan chopper kuadran satu sebagai konfigurasi sistem, dengan menambahkan kontrol kecepatan dan kontrol arus pada motor DC. Kecepatan pada motor DC diatur menggunakan tegangan jangkarnya, dalam hal ini dapat dilakukan dengan penyearah terkendali. Kecepatan pada motor DC dikontrol dengan menggunakan metode PI (Proportional-Integral). Pada penelitian ini kontrol PI difungsikan sebagi pengotrol kecepatan awal motor DC. Hasil pengontrolan pada motor DC merupakan nilai arus refrensi (Iref). Arus refrensi ini akan dibandingkan dengan arus jangkar untuk mengatur pencatuan thrysistor. Pengaturan close-loop akan menghasilkan kecepatan yang dapat dipertahankan untuk beban dinamis.
IMPLEMENTASI METODE HISTERISIS KONTROL ARUS UNTUK PENGATURAN KECEPATAN DAN TORSI PADA MOTOR DC CHOPPER
Authors: Prasetyo Johan
Published: January 2022

bstract -  Motor DC merupakan elemen mesin listrik yang mempunyai fungsi pada dunia industri seperti pabrik kertas, pabrik tekstil, penggulung tambang katrol dan digunakan juga sebagai motor servo untuk penentuan posisi dan pelacakan. Lambat laun penggunaan dari motor dc tersebut akan mengalami permasalahan juga. Permasalahan yang sering muncul pada pengaturan kecepatan motor dc yaitu kurangnya akurasi hasil referensi kecepatan yang diinginkan sehingga terjadi ketidak optimalan dalam pengoperasian di industri yang menggunakan elemen motor dc sebagai penggeraknya. Berdasarkan permasalahan tersebut maka dilakukan implementasi metode histerisis kontrol arus untuk menganalisa tingkat keakurasian dalam pengaturan kecepatan motor dc, dimana pada pengujian ini menggunakan nilai sampel beban dan torsi yang berbeda. Hasil pengujian yang sudah dilakukan menunjukan bahwa Voltage Source Converter (VSC) yang dirancang dalam penelitian ini telah bekerja dengan baik sesuai dengan tujuan pengguna. Hal ini dilihat dari grafik keluaran  arus, tegangan, dan arus error menggunakan metode histerisis kontrol arus.
Classical and Communicative Sign Models in Explanation of the Grelling – Nelson Paradox Part I
Authors:   Institute of Linguistics, Russian Academy of Sciences, Vdovichenko Andrey V. ,   Saint Tikhon’s Orthodox University of the Humanities
Published: January 2022

К.Grelling – L.Nelson’s paradox arises if the author (authors) and the addressee (addressees) use the classical linguistic model of meaning formation, which pos­tulates that self-referential signs (words), or modules “form – meaning”, are em­ployed and understood in word-containing semiotic procedures. The commu­nicative model of meaning formation, on the contrary, states the impossibility of an autonomous reference of a sign, considers an attempt at indirect influence (i.e., process) as the only possible object of reference and understanding in a given communicative act, recognizes the actional (“influential”) mode of semiotic ac­tor’s consciousness as the exclusive source of the produced “meaning”, includ­ing of sign reference. The paradox is viewed as a sequence of attempts at com­municative influences on a conceivable addressee who understands (approves or disapproves) the actions of the author of the paradox by considering his “influen­tial” cognitive states: on which objects the author focuses the addressee’s atten­tion, what are the denotations hidden by the author behind the “bodies” of signs, how justified the established classes, relationships, links, etc. The main vulnera­bility of the paradox is proposed to be seen in the fact that the author unjustifi­ably recognizes the independent out-of-communicative ability of a sign (word) to produce influences (to name, to describe, to have its own properties), does not notice the communicative constants of meaning formation in the theoretical field, although inevitably uses them practically. The paradox is eliminated to­gether with the removal of the classical concept of a sign as an autonomous module “form – meaning”.
Can We Hope for Progress in Understanding Progress?
Authors:   Institute of Philosophy, Russian Academy of Sciences, Stoliarova Olga E.
Published: January 2022

The article discusses the question raised by A.L. Nikiforov about the meaning and significance of scientific progress. It is shown that scientific progress, in ac­cordance with the original meaning embedded in this concept, should be consid­ered in the context of the universal development of the human reason, which covers not only the cognitive assimilation of the natural world, but also the con­struction of a harmonious society, and the improvement of man as such. Based on this, it is problematic to talk about autonomous scientific progress that does not affect the spiritual sphere. It is shown that changes in the understanding of progress refer not only to scientific or social progress, but to the whole com­plex of beliefs associated with the idea of the world development. The author traces the historical transformation of the three key concepts underlying the dis­course of progress, which the researcher of the metaphysics of progress Alain de Benoist identifies as the most stable. First, the idea of linear progress, based on the mechanistic ontology and reductionism, is replaced by the concept of so­cial evolution, based on the interaction of organic systems and subsystems. Sec­ondly, the idea of the fundamental unity of humanity and a single science gives way to an irreducible multiplicity of cultures and metaphysics. Third, the idea of a controlled transformation of the world is replaced by the concept of uncer­tainty. The author traces the transformation of the idea of progress into the idea of the complication of the world. A characteristic feature of the discourse of complication is that it speaks of a distributed agent of the world process, thereby relieving man of exclusive responsibility for the course of history. The author defends the point of view that changes in the understanding of progress are an expression of increasing conceptual and ontological complexity.
Sociological Skepticism toward Scientific Progress
Authors:   Institute of Philosophy, Russian Academy of Sciences, Argamakova Alexandra A.
Published: January 2022

Progress, truth, and rationality form a basic vocabulary for epistemology and philosophy of science. Sociological investigations of scientific knowledge, start­ing from the works of Thomas Kuhn, Barry Barnes and David Bloor, eliminate these traditional and central categories of philosophy. Their profound works be­came the source for the new types of scepticism toward scientific cognition, its proclaimed goals and received results. Within sociological studies of knowledge, science is researched as an integral part of culture. The scientific belief is deter­mined not only by achievements of reason, transcendental conditions, ways of perception or acquired experience, but rather by social authority, collec­tive agreements and distribution of power. In the article “What is scientific pro­gress?” Alexander Leonidovich Nikiforov outlines perspectives for compromise in sociological and philosophical thinking in order to overcome relativism and sceptical conclusions toward scientific progress. After all, scientific and social progress is not the unconditional ideal for humankind. It means only that scien­tific progress requires a new justification, especially in the light of synthesis be­tween philosophical and sociological methodologies. Another important aspect, stressed by Nikiforov, interprets the progress within the socio-humanitarian field. In the future, it is surely possible to expect the results from human sciences, whereas the resources of natural history evaporate and arrive to the last frontier. The most difficult is the knowledge of ourselves. Perhaps, it explains why the progress in human sciences has been gaining strength and promising the visi­ble, quite instrumental outbreaks.
“Green” Trend of Developing Culture of Future
Authors:   Institute of Philosophy, Russian Academy of Sciences, Strugovshchikova Ulyana S.
Published: January 2022

The Anthropocene, as a transitional geological era, is characterized by growing human activity that changes and destroys Earth's ecosystems. The ecological conception of a dialogue between humans and nature is ripening, where nature is an equal actor of coevolution of humankind and the living on our planet. For more than 50 years, we have gone from a green-house concept to plant-powered light bulbs, a tire concept using moss to purify urban air, and digital devices that “communicate” with plants. The connection of biology and technologies leads to the new bio-disciplines’ formation, which plays a remarkable role in our understanding of life, its bonds, targeting and meaning, and the develop­ment of sustainable ecosystems with various communicative connections of dif­ferent kinds. There is an influential developing movement whose task is to transform the relationship between humans and animals. New results in plant biology let us raise plants to a new level as agents. New ethical and legal issues emerge plant forests-sanctuaries are being created. Some issues of interdiscipli­nary communication and human communication with living systems are consid­ered as well as possible ways of their solution with the help of TRIZ and biosemiotics. An issue of distinguishing progress and sustainability in eco-tech­nological context is set up.
Education in the Age of Marginalism and the Alienation of Knowledge
Authors:   Institute of Oriental Studies, Russian Academy of Sciences, Zakharov Anton O. ,   Moscow State University of Psychology and Education
Published: January 2022

Global changes of contemporary education are so large-scale that they need a se­rious reconsideration. Philosophy of education has viewed education as a highly valued human activity and form of socialization, though philosophers may have had different views on the aims and methods of education. The importance of ed­ucation was based on the fact that a society could develop if and only if its mem­bers had an appropriate education. Nowadays education is discussed in journals, like the Philosophy of Education published in Novosibirsk, in annual confer­ences and by various associations of thinkers, teachers and professors. The abso­lute majority of philosophers since Plato emphasizes the fundamental value of education and wants to make its quality better. But a current state of humankind does not require a universal education of population. Robots and neural networks force the humans out of production. The humans are lacking a long-time mem­ory as information turns easily accessible by means of Internet. Growth rates of innovations, discoveries and changes are so high that adaptation to them requires daily efforts of any individual. Many professions are dying. The current trend of social development is growing marginalization of humans, their societies and countries. Knowledge alienates from its creators and, in a much greater degree, from public. Humans are first of all consumers but consumers do not need edu­cation: why one should spend many years in school and institute to buy food or see a clip on a smartphone? A multistage system of education looks outdated. The need of such an institution is dying step by step, as complex operations may be more effectively made by machines with stable, reliable and efficient neural networks, than by humans who have to spend years to become specialists. Colos­sal growth of information makes its learning by an individual impossible. Alien­ated knowledge turns external force regarding an individual.
Identification of Law in Modern Social Regulation
Authors:   Institute of Legislation and Comparative Law under the Government of the Russian Federation, Khabrieva Taliya Y.
Published: January 2022

Empirical studies of the dynamics of law have fixed the “growth points” of new directions in its evolution, characterizing the general vector of development, largely due to the hybridization of the legal landscape. It is formed by the so­cially significant interaction of a person adapted to an unusual environment, in­cluding through his own technological upgrade, hybrids of nature and culture (quasi-objects, “subject-objects”), which are overcoming the boundaries between culture and nature, the actor and the material. Theoretical understanding of the obtained data leads to the conclusion about the formation of a new “paradigm” of the evolution of law, which determines its hybridization as a result of conver­gence with technological innovations and practices; transformation from a norm­ative to an automated normative-cognitive system; convergence of the content and form of law, normative and individual legal regulation; modification of a number of its features, such as normativity and formal certainty; revision of the ideological foundations of law, its fundamental principles – humanism, free will, equality and justice. The depth of the corresponding transformations de­pends on what place the ideas that make up the content of these principles will occupy in the value system of the new society.
LncRNA NEAT1 Facilitates Glioma Progression Via Stabilizing PGK1
Authors: Liang Jingshan , Liu Changtao , Xu Dezhi , Xie Kang , Li Aimin
Published: January 2022

Background: Long noncoding RNA NEAT1 has been implicated in glioma progression. However, the effect of NEAT1 on glycolysis of glioma cell and the potential mechanism remain unclear.Methods: In vitro experiments, including CCK-8, colony formation, ECAR, and lactate detection assays were performed to evaluate the effect of NEAT1 on proliferation and glycolysis of glioma cell. RNA pulldown and RIP assays were performed to identify the interaction between NEAT1 and PGK1. Truncated mutation of NEAT1 and PGK1 was used to confirm the specific interactive domains between NEAT1 and PGK1. Animal studies were performed to analyze the effect of NEAT1/PGK1 on glioma progression. Results: NEAT1 knockdown significantly suppressed the proliferation and glycolysis of glioma cells. NEAT1 could specifically interact with PGK1, which promotes PGK1 stability. Hairpin A of NEAT1 is essential for interaction with M1 domain of PGK1. Depletion of NEAT1 markedly inhibited tumor growth in mice, while PGK1 could reverse this effect. Higher expression of NEAT1 was associated with poor overall survival of GBM patients.Conclusions: NEAT1 over expression promotes glioma progression through stabilizing PGK1. NEAT1/PGK1 axis is a candidate therapeutic target for glioma treatment.
The Survival Rate of Extremely Low Birth Weight Infants Improved in Guangdong Province, China
Authors: Jia Chun-Hong , Feng Zhou-Shan , Lin Xiao-Jun , Cui Qi-Liang , Han Sha-Sha , Jin Ya , Liu Guo-Sheng , Yang Chuan-Zhong , Ye Xiao-Tong , Dai Yi-Heng , Liang Wei-Yi , Ye Xiu-Zhen , Mo Jing , Ding Lu , Wu Ben-Qing , Chen Hong-Xiang , Li Chi-Wang , Zhang Zhe , Rong Xiao , Huang Wei-Min , Shen Wei , Yang Bing-Yan , Lv Jun-Feng , Huo Le-Ying , Huang Hui-Wen , Rao Hong-Ping , Yan Wen-Kang , Yang Yong , Ren Xue-Jun , Liu Dong , Wang Fang-Fang , Diao Shi-Guang , Liu Xiao-Yan , You Chu-Ming , Meng Qiong , Wang Bin , Zhang Li-Juan , Huang Yu-Ge , Ao Dang , Li Wei-Zhong , Chen Jie-Ling , Chen Yan-Ling , Li Wei , Chen Zhi-Feng , Ding Yue-Qin , Li Xiao-Yu , Huang Yue-Fang , Lin Ni-Yang , Cai Yang-Fan , Wan Zhong-He , Ban Yi , Bai Bo , Li Guang-Hong , Yan Yue-Xiu , Wu Fan
Published: January 2022

Background

With the increase in extremely low birth weight (ELBW) infants, their outcomes received special attention. However, in China, studies of the outcomes of ELBW infants are rare.
Methods

The hospitalized records of ELBW infants discharged from twenty-six neonatal intensive care units were reviewed and analyzed.
Results

A total of 2575 ELBW infants were enrolled and the overall survival rate was 55.11%. From 2008 to 2017, the number of ELBW infants increased rapidly from 91 to 466, and the survival rate improved steadily from 41.76–62.02%. The survival rate rose with increasing BW, the ascending level of regional economic development and specialist hospitals. The incidence of complications was neonatal respiratory distress syndrome (85.2%), bronchopulmonary dysplasia (63.7%), retinopathy of prematurity (39.3%), intraventricular hemorrhage (29.4%), necrotizing enterocolitis (12.0%), and periventricular leukomalacia (8.0%). Among the 1156 nonsurvivors, 90.0% of infants died during the neonatal period (≤ 28 days), and the other died after the neonatal period. A total of 768 ELBW infants died after medical care withdrawal, with economic factors and expected outcome being important causes.
Conclusion

The number of ELBW infants is increasing in China, and the overall survival rate is still low but is improving steadily.
Safe Provision of Systemic Anti-Cancer Treatment for Urological Cancer Patients During COVID-19: A Tertiary Centre Experience in the First Wave of COVID-19
Authors: So Alfred Chung Pui , Karampera Christina , Khan Muhammad , Russell Beth , Moss Charlotte , Monroy-Iglesias Maria J , Thillai Kiruthikah , Josephs Debra Hannah , Pintus Elias , Rudman Sarah , Hemelrijck Mieke , Dolly Saoirse , Enting Deborah
Published: January 2022

Background

Safe provision of systemic anti-cancer treatment (SACT) during the COVID-19 pandemic remains an ongoing concern amongst clinicians.
Methods

Retrospective analysis on uro-oncology patients who continued or started SACT between 1st March and 31st May 2020 during the pandemic (with 2019 as a comparator).
Results

441 patients received SACT in 2020 (292 prostate, 101 renal, 38 urothelial, 10 testicular) compared to 518 patients in 2019 (340 prostate, 121 renal, 42 urothelial, 15 testicular). In 2020, there were 75.00% fewer patients with stage 3 cancers receiving SACT (p<0.0001) and 94.44% fewer patients receiving radical treatment (p=0.0019). The number of patients started on a new line of SACT was similar between both years (118 in 2019 vs 102 in 2020; p=N.S) but with 53.45% fewer patients started on chemotherapy in 2020 (p=0.00067). Overall, 5 patients tested positive for COVID-19 (one asymptomatic, two moderate pneumonitis, one severe pneumonitis). Compared to 2019, 30-day mortality was similar (1.69% in 2019 vs 0.98% in 2020; p=N.S) whereas the 6-month mortality was lower (9.32% in 2019 vs 1.96% in 2020; p=0.023) in 2020.
Conclusion

This single-centre study demonstrated that uro-oncology patients can safely receive SACT during COVID-19 pandemic with a low incidence of infection and mortality.
High Pressure and Polaron Effects on Optical Properties of GaN Wedge-Shaped Quantum Dot
Authors: Sayari A. , Servatkhah M. , Pourmand R.
Published: January 2022

In the paper, the optical properties of GaN wedge-shaped quantum dot (WSQD) under influence both pressure and electron-phonon (e-p) interaction have been investigated. To this end, the energy difference and dipole matrix elements are evaluated by considering the effects of e-p and pressure. Then, the refractive index changes (RICs) and absorption coefficients (ACs) are evaluated. It is deduced that the energy difference and dipole matrix elements reduce by considering the e-p and pressure effects. It is resulted that the pressure enhances the e-p effect on the energy difference. The ACs and RICs move to lower energies by considering the e-p and pressure effects. The RICs and ACs are raised in the presence of e-p effect whereas they are reduced with considering the pressure. With considering the e-p and pressure effects on total ACs, it is seen that the peak of AC splits into two peaks.
ERBB3 Methylation and Immune Infiltration in Tumor Microenvironment of Cervical Cancer
Authors: Yang Xiaoyue , Chen Ying , Li Mei , Zhu Weipei
Published: January 2022

ERBB3, a member of the ERBB family of receptor tyrosine kinases, plays an important role in cancer, despite its lack of intrinsic Carcinogenic mechanism of CESC. Research on bioinformatics methods through multi-omics, this work proves that ERBB3 gene mutation, methylation modification have extensive regulatory mechanisms on the CESC microenvironment. We found that ERBB3 is involved in carcinogenesis of cervical cancer and is not associated with its prognosis. The carcinogenic mechanism is mainly related to the suppression of the immune system between TILS and the methylation of the RNA level. Our study indicated ERBB3 is more likely to be a carcinogenic factor than a key prognostic factor for cervical cancer. Methylation of ERBB3 may work as a chekpoint immunotherapy target in CESC, DNA methylation modification of the 4480 base pair downstream of ERBB3 transcription initiation site was the highest.
Association of HMGB1, S100A12 and IL-17A Expression with Immunoglobulin-Resistant in Kawasaki Disease and the Comparisons Between Different Adjunctive Therapeutic Approaches
Authors: Zhou Yang , Pan Liu , Yang You-jun , Li Shi-yu , Yin Wei , Liu Fan , ding yan
Published: January 2022

Objective: The DAMPs such as HMGB1, S100A12 and IL-17A have been reported to predict poor response to IVIG. The aim of this study was to analyze the role of HMGB1,S100A12 and IL-17A in the detection of inflammation in KD patients with IVIG-resistant, and to investigate the value of different adjunctive therapy.Method: This study enrolled 126 patients diagnosed with KD, as well as age-matched 16 febrile control subjects. The demographic or clinical data, laboratory parameter and blood sample were collected. Various laboratory parameters as predictive factors for IVIG-resistant were calculated. And the serum levels of IL-17A and mRNA expression levels of HMGB1 and S100A12 were tested in all patients. For patients with acute KD in IVIG-resistant, we studied the levels of laboratory variables when using of IVIG retreatment, methylprednisolone, infliximab for children patients. Result: The variance of laboratory parameters between the febrile control group and KD group were analyzed. Regarding laboratory parameters, KD individuals were found to have lower levels of L%, PA, CD4+, CD8+ and higher levels of WBC, N%, CRP, ESR, NT-proBNP, ALT, CD4+/CD8+ (P<0.05 or P<0.01). For KD group, the 53 IVIG-resistant patients had significantly higher levels of blood S100A12, HMGB1, serum IL-17A levels And N%, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT (P<0.05 or P<0.01) in comparison to the IVIG-responsive patients. For patients with acute KD in IVIG-resistant, after initial IVIG-treatment, the adjunctive therapy of IVIG, methyl prednisolone or infliximab were used, the inflammatory symptoms and laboratory inflammatory markers were improved when treated with those drugs. Conclusion: IVIG-resistant was associated with higher levels of HMGB1, S100A12, IL-17A, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT before IVIG, especially when combined, were useful predictors for IVIG-resistant in KD. In addition, the adjunctive therapy of methylprednisolone and infliximab showed more effective in relief clinical symptoms than IVIG retreatment.
Multi-Omics Study of The Salivary Modulation of The Rumen Microbiome
Authors: Palma-Hidalgo Juan Manuel , Belanche Alejandro , Jiménez Elisabeth , Martín-García A. Ignacio , Newbold Charles J. , Denman Stuart E. , Yáñez-Ruiz David R.
Published: January 2022

Ruminants are able to produce large quantities of saliva which enter into the rumen. Although previous research has indicated that salivary immunoglobulins can partially modulate the rumen microbial activity, the role of the salivary components other than ions on the rumen microbial ecosystem has not been thoroughly investigated in ruminants. A total of 16 semi-continuous in vitro cultures were used to incubate rumen fluid from 4 donor goats inoculated with autoclaved saliva (AUT) as negative control, saliva from the same rumen fluid donor (OWN) as positive control, and either GOAT or SHEEP saliva as experimental interventions. Fermentation was monitored throughout the 7 days of incubation and the prokaryotic communities and metabolome were analysed at day 7 of incubation. Characterization of the salivas used prior to incubation showed a high degree of individual variability in terms of the salivary metabolites and proteins, including immunoglobulins. The prokaryotic community composition in AUT incubators was the most divergent across treatments, suggesting a modulatory effect of active salivary components, which were not affected in the other treatments (OWN, GOAT and SHEEP). The differences across treatments in microbial diversity were mostly caused by a greater abundance of Proteobacteria and Rikenellacea and lower of Prevotellaceae, a key rumen bacterium with greater abundance in GOAT and SHEEP treatments. These results suggest that specific salivary components contribute to host-associated role in selecting the rumen commensal microbiota and its activity.
Nanocluster Mediated Photothermia Improves Eradication Efficiency and Antibiotic Sensitivity of Helicobacter Pylori
Authors: Meng Fansen , Tao Hongjin , Mi Yan , Yang Tianyu , Wang Xuanping , Go Yuyo , Lin Yunjuan , Wang Gangshi
Published: January 2022

Background: Helicobacter pylori (H. pylori) eradication plays a crucial role in gastric cancer prevention, but the antimicrobial resistance of H. pylori is obstructing this elimination process. In this study, we developed nanoclusters (NCs) from Zn0.3Fe2.7O4 nanoparticles using a poly(ethylene glycol)-b-poly(ε-caprolactone)-based nanocarrier as an innovative antibiotic-independent H. pylori management.Results: The nanocluster showed minimal toxicity and maximal biocompatibility. With a low concentration (50 µg/mL) of NCs under a short time period (～2 min) of near-infrared (808nm) irradiation, we kept the culture medium temperature to 41 °C for 20 minutes with continuous irradiation. The heated NCs exhibited efficient photothermal effects and resulted in an excellent inhibition of H. pylori growth, adhesion ability and cell vacuolization ability in in vitro investigation. Transmission electron microscopy showed a dramatic morphologic change after NCs photothermia on H. pylori, including cell wall and membrane rupture, as well as ribosome damage. Besides, levofloxacin and clarithromycin resistance were improved after photothermal treatment in H. pylori NCTC 11637 and/or clinical strains, however metronidazole resistance was unchanged. We also discovered a significant decrease in the biofilm formation of H. pylori under the NCs-based photothermal application, while efflux pump function was unchanged.Conclusions: Based on this novel NCs-based photothermal approach, we were able to demonstrate in vitro a significant inhibition of both H. pylori growth and molecular toxicity, and its improvement in antibiotic resistance alone with the eradication of H. pylori biofilms previously believed to be resistant to conventional antibiotics.
Comparison of Low-Cost Methods for Soil Water Holding Capacity
Authors: Govindasamy Prabhu , Mahawer Sonu Kumar , Mowrer Jake , Bagavathiannan Muthukumar , Prasad Mahendra , Ramakrishnan Srinivasan , Halli Hanamant M , Kumar Sunil
Published: January 2022

Purpose: The use of cost-effective methods for measurement of WHC is common in underdeveloped and developing countries, but the accuracy of these cost-effective methods compared to the sophisticated and more expensive alternatives is unclear. Methods: To compare different WHC measurement methods, 30 random samples of clay loam and sandy clay loam soils of Jhansi, India were used. The methods compared here were: FAO in-situ method (FAO), Keen Raczkowski box method (KM), funnel method (FM), column method (CM) and pressure plate method (PPA). Results: For WHC measurements the PPA results were comparable to KM and FM methods for sandy clay loam, and KM and FAO methods for clay loam. Conclusion: Therefore, until a reliable method that matches the results of sophisticated analytical methods of soil water measurement is available, different inexpensive analytical methods can be used, but they must be chosen with caution. The findings from this study will facilitate appropriate selection of a suitable method.
Spatiotemporal Characteristics of Meteorological Drought During the Past Half Century in Different Climates Over Iran
Authors: Ghaleni Mehdi Mohammadi , Sharafi Saeed , Hosseini-Moghari Seyed-Mohammad , Helali Jalil , Oskouei Ebrahim Asadi
Published: January 2022

The present study compares the main characteristics (intensity, duration, and frequency) of meteorological drought events in the four climates (Hyperarid, Arid, Semiarid, and Humid) of Iran. For this purpose, three drought indices, including Standardized Precipitation Index (SPI), Reconnaissance Drought Index (RDI), and Standardized Precipitation Evapotranspiration Index (SPEI), were employed at the timescales of 1-, 3-, 6-, 9- and 12-months. These indices were compared by utilizing long-term data of 41 synoptic meteorological stations for the recent half century, 1969–2019. The long-term analysis of drought indices indicates that the duration and intensity of drought events have temporally risen after the 1998–99 period. Iran has experienced the longest duration (40 months) of extreme drought during Dec 98–Mar 02 and Jan 18–Mar 18, respectively. Spatial patterns demonstrate that drought intensity uniformly increased in SPI1 to SPI12, and SPEI3 to SPEI12, from humid and semiarid to arid and hyperarid regions. The average drought duration in studied stations for SPI, SPEI and RDI indices equaled 9, 12, and 9 months, respectively. In addition, mean drought frequencies are calculated at 14, 17, and 13 percent for SPI, SPEI and RDI indices, respectively. Generally, SPEI compared to SPI and RDI shows greater duration and frequency of drought events, particularly in arid and hyperarid regions. The research shows the crucial role of climatic variables in detecting drought characteristics and the importance of selecting appropriate drought indices in various climates.
SLGT2 Inhibitor Rescues Myelopoiesis in G6PC3 Deficiency
Authors: Hiwarkar Prashant , Bargir Umair , Pandrowala Ambreen , Bodhanwala Minnie , Thakker Naresh , Taur Prasad , Madkaikar Manisha , Desai Mukesh
Published: January 2022

The energy metabolism of myeloid cells depends primarily on glycolysis. 1,5-anhydroglucitol (1,5AG), a natural monosaccharide is erroneously phosphorylated by glucose-phosphorylating enzymes to produce 1,5-anhydroglucitol-6-phosphate (1,5AG6P), a powerful inhibitor of hexokinases. The endoplasmic reticulum transporter (SLC37A4/G6PT) and the phosphatase G6PC3 co-operate to dephosphorylate 1,5AG6P. Failure to eliminate 1,5AG6P is the mechanism of neutrophil dysfunction and death in G6PC3-deficient mice. SLGT2-inhibitor reduces 1,5AG level in the blood and restores the neutrophil count in G6PC3-deficient mice. In the investigator-initiated study, a 30 year-old G6PC3-deficient woman with recurrent infections, distressing gastrointestinal symptoms and multi-lineage cytopenia was treated with an SLGT2-inhibitor. A significant increase in all the hematopoietic cell lineages and substantial improvement in the quality of life was observed.
Clinical Performance of Friend Leukemia Virus Integration 1 Methylation as a Biomarker for Colorectal Carcinoma
Authors: Wang Jing , Xue Cai-Lin , He Yuan
Published: January 2022

Objectives

Accumulating evidence supports the reliability of molecular biomarkers and cancer, the current situation is lacking. This present study was aimed to investigate clinical performance of friend leukemia virus integration 1 (FLI1) methylation as a biomarker for colorectal cancer (CRC).
Methods

The datasets of UALCAN, GEPIA, cBioPortal, STRING, TIMER2.0, GEO, and KOBAS were utilized in this present study. In order to testify the methylation function of FLI1 in CRC, we studied 6 CRC cell lines and 20 pairs of tumor tissues. The transcriptional levels of FLI1 were tested by reverse transcription PCR quantitatively and western blot. Cell viability, transwell assays and plate clone assay were utilized to assess the cell function.
Results

FLI1 was up-regulated in the GBM, KIRC, LAML, etc. Meanwhile, it was down-regulated in BLCA, BRCA, CESC, etc (p<0.05). The CPTAC dataset showed higher total protein in the primary tissues of KIRC, lower protein in the BRCA, OV, LUAD, UCEC than normal tissues(p<0.05). Highly expressed FLI1 was linked to poor prognosis of overall survival (OS) in LGG, UVM (p<0.05). Low expression of FLI1 was associated with short OS in KIRC, LUAD, SKCM (p<0.05). Compared with normal tissues, the methylation level of FLI1 was increased in BRCA, CESC, CHOL, COAD, etc (p<0.05). In the contrary, it was decreased in KIRP, PCPG obviously (p<0.05). Moreover, it showed a reduced phosphorylation for selected probes (BRCA: NP_002008.2:S39, NP_002008.2:S3241; OV: NP_002008.2:S39; LUAD: NP_002008.2:S79, NP_002008.2:S3241; all p<0.05). Meanwhile, it showed an enhanced phosphorylation level of FLI1 in KIRC for selected probes (NP_002008.2:S241, p<0.05). Besides, a statistical negative correlation was found between FLI1 and Treg cells, neutrophils, monocytes, macrophages, NK cells, cancer-associated fibroblasts, and common immune checkpoint gene levels (p<0.05). Besides, in vivo experience showed that 5-Aza-2’-deoxycytidine diminished FLI1 methylation level and restored transcriptional levels (p>0.05) in CRC. In vitro experiments demonstrated that the proliferation, colony formation, invasion and migration of CRC cells were inhibited by FLI1 overexpression through FMNL1 (p<0.05).
Conclusions

This present study offers a comprehensive understanding of FLI1 in carcinomas with oncogenesis and immunotherapeutic implications. Moreover, FLI1 reduced the proliferation, colony formation, invasion and migration of CRC by FMNL1.
An Unusual Presentation of Cutaneous Alternariosis with Bullous Lesions in A Patient with Diabetes Mellitus
Authors: wang xiaopo , Ling Shiqi , Liang Guanzhao , Sun Jianfang
Published: January 2022

Diabetes mellitus considered to represent an immunodeficient state significantly predisposes patients to all types of opportunistic invasive fungal infection. However, very few cases of cutaneous alternariosis associated with diabetes have been reported previously. Herein, we describe a rare case of cutaneous alternariosis in an elderly diabetic patient with antecedent local trauma.
Synthetic MRI for Stroke: A Qualitative and Quantitative Pilot Study
Authors: André Joachim , Barrit Sami , Tchofo Patrice Jissendi
Published: January 2022

PurposeSynthetic MR provides quantitative multiparametric data about tissue properties in a fast single-acquisition. We compared synthetic and conventional image quality and investigated synthetic relaxometry of acute and chronic ischemic lesions to support its interest in stroke imaging. MethodsFor this pilot study, we prospectively acquired synthetic and conventional brain MR of 43 consecutive adult patients with suspected stroke. We studied a total of 136 lesions, of which 46 DWI-positive with restricted ADC (DWI+/rADC), 90 white matter T2/FLAIR hyperintensities (WMH), and 430 normal brain regions (NBR). We assessed image quality for lesion definition according to a 3-level score by two readers of different experiences. We compared relaxometry of lesions and regions of interest.Results Synthetic images were superior to their paired conventional images for lesion definition except for sFLAIR (sT1 or sPSIR vs. cT1 and sT2 vs. cT2 for DWI+/rADC and WMH definition; p-values <.001) with substantial to almost perfect inter-rater reliability (κ ranging from 0.711 to 0.932, p-values <.001). We found significant differences in relaxometry between lesions and NBR and between acute and chronic lesions (T1, T2, and PD of DWI+/rADC or WMH vs. mirror NBR; p-values <.001; T1 and PD of DWI+/rADC vs. WMH; p-values of 0.034 and 0.008).Conclusion Synthetic MR may contribute to stroke imaging by fast acquiring consistent relaxometry data and accessible derived images of interest for the study of ischemic lesions.
Performance of PROPELLER FSE T2WI in Reducing Metal Artifacts for Patients with Various Material Porcelain Fused To Metal Crown: A Preliminary Study
Authors: Li Wenjin , Shi Jing , Bian Wenjin , Li Jianting , Chen Xiaoqing , Feng Juan , Yu Jiali , Wang Jun , Niu Jinliang
Published: January 2022

This study aimed to compare MRI quality between common fast spin echo T2 weighted imaging (FSE T2WI) with periodically rotated overlapping parallel lines with enhanced reconstruction (PROPELLER) FSE T2WI for patients with various porcelain fused to metal (PFM) crown and analyze the value of PROPELLER technique in reducing metal artifacts. Common FSE T2WI and PROPELLER FSE T2WI sequences for axial imaging of head were applied in participants with different PFM crowns: cobalt-chromium (Co-Cr) alloy, pure titanium (Ti), gold-palladium (Au-Pd) alloy. Two radiologists evaluated overall image quality of section in PFM using a 5-point scale qualitatively and measured the maximum artifact area and artifact signal-to-noise ratio (SNR) quantitatively. The metal crown with the least artifacts and the optimum image quality shown in common FSE T2WI and PROPELLER FSE T2WI were in Au–Pd alloy, Ti, and Co–Cr alloy order. PROPELLER FSE T2WI was superior to common FSE T2WI in improving image quality and reducing artifact area for Co-Cr alloy (17.0±0.2% smaller artifact area, p＜0.001) and Ti (11.6± 0.7 % smaller artifact area, p=0.005), but had similar performance compared to FSE T2WI for Au-Pd alloy. For all PFMs, PROPELLER FSE T2WI significantly reduced the signal-to-noise ratio (SNR) of artifact (393.57±89.75 VS. 214.05±70.45, p < 0.001) when compared to common FSE T2WI.Therefore, the different PFM crown generate varying degrees of metal artifacts in MRI, and the PROPELLER can effectively reduce metal artifacts especially in the PFM crown of Co-Cr alloy.
Use of PiggyBac Transposon System Constructed Murine Breast Cancer Model For Reporter Gene Imaging And Characterization of Metastatic Tumor Cells
Authors: Chen Ying-Ling , Hou Kuei-Yuan , Lin Min-Ying , Lin Yu-Chuan , Chuang Hui-Yen , Lee Yi-Jang
Published: January 2022

The piggyBac transposon system is known to non-viral integrate exogenous genes to chromosomes of mammalian cells. For reporter gene imaging, this transposon system is believed to efficiently establish xenograft tumor model with low immunogenicity. Because tumor cells usually exhibit genomic instability, it is important to investigate if piggyBac mediated transduction of reporter genes would change tumor characteristics. In this study, reporter gene imaging mediated by the piggyBac transposon system was exploited to track the growth and dissemination of 4T1 triple-negative murine breast cancer cells in vivo, followed by ex vivo analysis of the metastatic cells expressing reporter genes. We demonstrated that several cell properties, including proliferation rate, invasion and migration rate, and mammosphere formation ability of 4T1 cells were not influenced by piggyBac transposon system. Further, we isolated the liver metastatic cells, named 4T1-3R_L cells for further analysis. Compared to parental 4T1 cells, 4T1-3R_L cells exhibited several cancer stem cells (CSC) related characteristics, including significant mammosphere formation ability, resistance to doxorubicin, high tumorigenicity potential in Balb/C mice and expression of CD44 CSC marker. We also found that 4T1-3R_L cells exhibited stronger migrated and invasive abilities, by wound healing assay and in vitro invasion assay, respectively. The cell adhesive ability of 4T1-3R_L cells was also lower than that of 4T1 cells. The microarray assay showed that several epithelial-mesenchymal transition (EMT) promoting markers, including vimentin, N-cadherin, Twist1, and Snail were up-regulated, and anti-EMT marker E-cadherin was down-regulated in 4T1-3R_L cells. Current data suggest that the piggyBac transposon system is a reliable and biocompatible tool to engineer cancer cells for tacking and characterizing tumor development in vivo and in vitro.