bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Liquid Biofertilizers on Productivity, Quality and Soil Biota of Fodder Sorghum
Authors: Yamalapalli Vamsi , Tirumala Reddy S.  , Raveendra Reddy M.  , Prathima T.
Published: September 2023

The field experiment was conducted during Rabi season of 2022 on sandy clay loam soils at wetland farm of the S.V. Agricultural College, Tirupati, Andhra Pradesh, India, to study the effect of liquid biofertilizers on productivity, quality, and soil biota of fodder sorghum. The treatments were allocated in randomized block design and replicated thrice. The soil was neutral in reaction (6.9 pH), low in available nitrogen (115 kg ha-1), medium in available phosphorus (29 kg ha-1) and low in available potassium (156 kg ha-1) status. The initial soil contains bacteria (13.08 X 107 CFU g-1 soil), fungi (4.04 X 104 CFU g-1 soil) and actinomycetes (1.54 X 103 CFU g-1 soil). The results revealed that the maximum green (32.1 t ha-1) and dry (13.0 t ha-1) fodder yield as well as crude protein (7.3%) were obtained with the application of 75% RDF + Azospirillum + PSB + KSB (Both seed & soil application). Furthermore, this treatment produced a significantly higher soil bacterial (23.2 X 107 CFU g-1 soil), fungal (13.7 X 104 CFU g-1 soil) and actinomycetes (10.3 X 103 CFU g-1 soil) population at harvest.
Estimation of Runoff by SWAT Model in Noyyal River Basin
Authors: Revathi A. R. , Selvakumar S. , Ravikumar V. , Kannan Balaji , Raju M.
Published: September 2023

Accurate assessment of water balance components holds paramount importance for the sustainable management of water resources in river basins. This study specifically concentrates on the application of the Soil and Water Assessment Tool (SWAT) model to evaluate water balance elements within the Noyyal River basin, a tributary of the Kaveri River originating from the Vellingiri hills in Western Tamil Nadu grapples with escalating water stress brought about by both anthropogenic activities and climatic fluctuations.

The methodology adopted encompasses the harmonious integration of diverse spatial and temporal datasets. These datasets are seamlessly incorporated within the confines of a Geographic Information System (GIS) framework. Through the process of watershed delineation 21 sub-basin was partitioned and which is further subdivided into 217 unique Hydrological Response Units (HRUs) are derived, each corresponding to a distinct hydrological reaction. The simulation employs 33 years of daily precipitation, coupled with minimum and maximum temperature records, enabling the continuous execution of the SWAT model for runoff prediction. The findings elucidate fluctuating runoff volumes over time, spanning from a minimum of 11.17 mm to a maximum of 610.32 mm. Notably, the annual average runoff stands at 206.7 mm, representing approximately 25.6% of the cumulative rainfall experienced within the Noyyal river basin.
Root Morphological Characteristics of Five Rice Genotypes with Different Nitrogen Use Efficiency
Authors: Saini Ravi , Manjaiah K. M. , Chobhe Kapil A. , Raju Dhandapani , Ranjan Rajeev , Bana R. S. , Kumar Asheesh , Patil Kalyani V.
Published: September 2023

While nitrogen (N) uptake in rice has been extensively studied, the influence of root morphology on this process is not fully elucidated. This study explores the root morphological characteristics and N uptake of five diverse rice genotypes under different N sources, including novel slow-release nanoclay polymer/biopolymer composite (NCPC/NCBPC) fertilizers. A greenhouse experiment was conducted using five diverse rice genotypes (Swarna, Pusa Basmati-1, Pusa-44, MTU1010 and Nagina-22) with five N treatments (T1- control (without N fertilizer), T2- 100% RDF of N through urea, T3- 75% RDF of N through urea loaded NCPC, T4- 75% RDF of N through urea loaded NCBPC-I (NCBPC prepared with wheat flour), T5- 75% RDF of N through urea loaded NCBPC-II (NCBPC prepared with maida)). The results of the study revealed that maximum overall root growth was recorded under Pusa Basmati-1 followed by Swarna, MTU 1010, Nagina-22 and Pusa-44. Among the N treatment, maximum root growth and N uptake was recorded under NCPC treatment followed by NCBPC-II, NCBPC-I, urea and control. Thus, the study reveals significant variations in root traits among genotypes and N treatments, with notable improvements observed under NCPC and NCBPC based N treatments.
Morphological and Physiological Analysis of VRI-3 Cashew Plantations under Different Planting Density Systems
Authors: Kumaresh V. , Rani M. S. Aneesa , Vethamoni P. Irene , Senthil A. , Uma D.
Published: September 2023

Cashewnut (Anacardium occidentale L.) is an export-oriented plantation crop. The VRI-3 cashew plantation, utilizing a 7 x 7 m low-density (LD) system with approximately 200 plants ha-1, falls short of meeting the country's import and export demands. Consequently, a high-density planting (HDP) system was developed in Tamil Nadu to enhance India's cashew production. Further advancement through ultra-high-density planting (U-HDP) has proven effective, achieving a plant population of about 1250–1600 plants per hectare. In addition to the increased number of plants in the U-HDP approach, morphological characteristics such as tree height, trunk height, trunk girth, and canopy ground coverage were studied. The impact of these morphological changes resulting from an increased tree population on physiological parameters like Leaf Area Index (LAI) and light extinction coefficient (k) was also analysed. This analysis was carried out using the ‘Ceptometer’ model AccuPAR LP-80 device, aiming to determine how these changes might interfere with productivity and subsequently influence the cashew plantation's production capacity.
Characteristics of Fly Ash and its Influence on the Germination of Amaranthus Seeds
Authors: Ramyalakshmi A. , Sheeba S. , Prabhaharan J. , Kumutha K. , Ananthan M.
Published: September 2023

The physical and chemical properties of fly ash collected from three different thermal power stations were studied and the fly ash with neutral to alkaline pH was utilized as a medium for the germination of amaranthus seeds. The germination test was carried out using a completely randomized design with two replications at Agricultural College and Research Institute, Madurai. The various fly ash was analyzed for its physical and chemical properties with standard methods. The fly ash with a slightly alkaline pH was selected and media was prepared with soil, vermicompost and farm yard manure. The seeds of an amaranthus crop were sown and the germination percentage, days to germination, shoot length, root length and vigour index were recorded. The collected fly ashes were recorded with low bulk density, alkaline pH with quantities of micro and macronutrients. The fly ash of Tuticorin has a slightly alkaline pH (8.03) and was utilized for media preparation. Among the different media, the media with treatment Fly ash + Soil + Vermicompost (1:1:4) + Bio Fertilizer has recorded higher germination percentage, shoot length, root length, vigour index, lower days to germination with 94.6%, 18.3 cm, 4.8 cm,16.17 and 2.8 respectively. The vermicompost is best for the enrichment of fly ash compared to farm yard manure as it is completely decomposed organic manure.
Soil Application of Sea Weed Extract Granules and Foliar Application of Nano ZnO to Bt Hybrid Cotton to Vertic inceptisols
Authors: Raju Ambati Ravinder
Published: September 2023

Bt hybrid cotton was significantly benefitted by the soil application of Sagarika sea weed extract granules at 25 kg ha-1 yr-1 along with the recommended basal fertilizers dose or foliar application of nano ZnO 4% at 0.04% at squaring and flowering stages. These treatments  significantly enriched index leaf residual  P and  Zn content beyond 20 μg g-1  fresh weight during boll development stage and this resulted in  significant improvement in lint yield by 7.5% i.e. 35 kg lint ha-1 yr-1, C:B ratio by 5.0% and net returns to the tune of ₹. 5000 ha-1 over alone 100% RDF in eroded Vertic inceptisols deficient in N, Mg, S, Zn and B. Excess rains at seedling and vegetative stage followed by drought at reproductive stages in both years, reduced the economic viability of seed treatment with bio-stimulants and foliar applications besides soil and foliar application of Zn, S, B alone or together to Bt hybrid cotton.
Exploring the Rotavirus Risk in Latin American Food and Water
Authors: Bourdett-Stanziola Lurys
Published: September 2023

Rotavirus is considered a major public health problem worldwide because many children, adults, and animals die from gastroenteritis due to rotavirus; rotavirus contamination follows the fecal/oral route, and it is well supported that infection can also be achieved by consuming food and water that is contaminated with rotavirus. Research has shown that, in Latin American countries, unusual emerging strains of rotavirus are occurring in children and adults with gastroenteritis; which contain in their sequence genes from rotavirus genotypes detected in animals; Therefore, researchers consider it a virus with zoonotic potential.

In this review, I propose that rotavirus transmission occurs from wild animals, who use an intermediate host before contaminating humans, by means of the fecal/oral route, food, and water. Rotavirus produces genetic rearrangements generating new emerging strains, which are excreted into the environment. Generally, during the dry season in our Latin American countries, contaminated water is used to irrigate crops, and from there, much of this water ends up in the sea, where rotavirus is absorbed by filter-feeding molluscs, and from there, consumed by man and animals continuing its cycle.

Studies on the molecular characterization of rotavirus strains detected in food and water provide new insights into possible rotavirus genetic rearrangements and zoonoses. The emergence of strains derived from interspecies transmission has implicated and inspired the study of different vaccine strategies.
Literatura Infantil na Escola:
Authors: Barbosa Amanda Basílio , Rostas Márcia Helena Sauáia Guimarães , Rostas Guilherme Ribeiro
Published: September 2023

O presente estudo tem como objetivo analisar a literatura infantil com base nas categorias de classe, gênero e raça. Para tanto, foi realizada uma revisão bibliográfica, de cunho qualitativo, tendo como principais referenciais: Almeida (1998), Davis (2016 e 2018), Dos Santos (2019), Engels (2019), Guareschi (2014), hooks (2018), Jovino (2006) e Zilberman (2003). Verificou-se que o livro destinado à criança, foi utilizado como um instrumento de propagação de ideologias dominantes em cada momento histórico. A figura da mulher esteve atrelada ao trabalho reprodutivo e a subalternidade masculina. Quando se considera a mulher negra, esta foi representada pela a literatura infantil nacional em três momentos distintos, de acordo com o contexto social de cada época. Da mulher preta trabalhadora doméstica, para a mulher parda com traços e atributos brancos, até chegar na configuração atual, que está aos poucos rompendo com os estereótipos depreciativos da população negra.
Ciberfeminismos em tempos de Pandemia:
Authors: Fernandes Carla Montuori , Botelho Marina Alvarenga , Antonioli Alícia Frota de Souza , Oliveira Luiz Ademir de
Published: September 2023

Este estudo tem como objetivo investigar práticas ciberfeministas nas redes sociais, especificamente no Twitter, durante a pandemia, identificando as principais redes de discussão e quais os tópicos mais abordados. Compreende-se a rede social como um espaço que amplia a esfera pública, e, portanto, como central às novas práticas feministas. As discussões baseiam-se, também, nos conceitos de cibercultura e ciberfeminismo, com foco no feminismo interseccional. O corpus de análise foi coletado utilizando-se o Netlytic, nos meses de janeiro e março de 2021. Como metodologia de análise dos dados, utilizou-se a Análise de Redes Sociais (ARS) e a análise de conteúdo. Já como resultados, observou-se que as práticas ciberfeministas vêm acontecendo de forma descentralizada e diminuída durante a pandemia.
Os Saberes Docentes e as formas de inserção curricular da História das Mulheres nos Planos de Aula do portal Nova Escola (2017-2019)
Authors: Carvalho Bárbara de Almeida
Published: September 2023

Este artigo analisa os saberes docentes mobilizados nos Planos de Aula que tratam da História das Mulheres, no portal educacional Nova Escola. Para essa análise foram escolhidos doze Planos de Aula, que integram o projeto “Planos de Aula NOVA ESCOLA” e que estão, de acordo com o portal, alinhados à Base Nacional Comum Curricular (BNCC). A partir da análise de três tabelas que consideram aspectos dos Planos, da BNCC e os discursos apresentados a cerca das mulheres. A partir dessa análise será pensada a questão da subjetivação feminina, quais os interesses, valores e práticas que foram escolhidos para a construção dos Planos em um portal de grande visibilidade.
Resenha: DELAP, Lucy. Feminismos: Uma história global. Tradução de Isa Mara Lando e Laura Teixeira Motta. 1º edição. São Paulo: Companhia das Letras, 2022.
Authors: Saraiva Samara Akemi
Published: September 2023

NÃO POSSUI, TRATA-SE DE RESENHA DE LIVRO
"Todo mundo vem pra Recife"
Authors: Rios Luís Felipe
Published: September 2023

Este artigo discute os circuitos de sociabilidade de homens que fazem sexo com homens (HSH) da Região Metropolitana do Recife (RMR). Os dados são oriundos de pesquisa etnográfica realizada por meio de entrevistas, observações participantes e inquérito comportamental, com uma amostra formada por 10 cadeias de respondentes. Nos municípios menores prevaleceu redes comunitárias de malha estreita, que controlavam as pessoas e estigmatizavam as homossexualidades. Para escapar do controle, a homossociabilidade acontecia em espaços como as casas de amigos, praias e praças. E, quando queriam a diversão das boates e bares gay, iam para Recife. Paralelamente, a dimensão online era acessada, esgaçando o controle e ampliando as possibilidades eróticas. Analisando a própria dinâmica do trabalho de campo, observamos uma maior facilidade de construir cadeias de respondentes a partir de bairros pobres de Olinda e Recife, que relacionamos às mudanças nas possibilidades de se mostrar homossexual.
The Intersection of Climate Justice and Criminal Justice
Authors: Haber Lawrence A. , Cloud David H. , Boudin Chesa , Williams Brie A.
Published: January 2025

This Viewpoint discusses the ways in which incarcerated individuals are negatively impacted by the consequences of climate change and steps carceral facilities and policymakers can take to mitigate extreme heat risks.
Disseminated Pseudovesicles in an Immunocompromised Patient
Authors: Nahm William J. , Juarez Michelle C. , Mazori Daniel R.
Published: January 2025

A 41-year-old immunocompromised man presented to the intensive care unit with neutropenic fevers, and after a 1-week stay, developed oral ulcers and tender, diffuse, erythematous pseudovesicular papules, many umbilicated, on the face, trunk, and extremities. What is your diagnosis?
Use and Spending on Medical Equipment Among US Cancer Survivors
Authors: Jafri Faraz I. , Patel Vishal R. , Qasim Hussaini Syed M. , Premnath Naveen , Parsons Helen M. , Blaes Anne H. , Gupta Arjun
Published: January 2025

This survey study assesses patterns of use and costs of medical equipment among US cancer survivors.
Medically Recommended vs Nonmedical Cannabis Use Among US Adults
Authors: Han Beth , Compton Wilson M. , Einstein Emily B. , Volkow Nora D.
Published: January 2025

This cross-sectional study assesses differences in cannabis use frequency and prevalence of cannabis use disorder between medical-only, medical-nonmedical, and nonmedical-only use among US adults.
Clinical significance of stratifying prostate cancer patients through specific circulating genes
Authors: Derderian Seta , Jarry Edouard , Santos Arynne , Vesval Quentin , Hamel Lucie , Sanchez‐Salas Rafael , Rompré‐Brodeur Alexis , Kassouf Wassim , Rajan Raghu , Brimo Fadi , Duclos Marie , Aprikian Armen , Chevalier Simone
Published: January 2025

Patient stratification remains a challenge for optimal treatment of prostate cancer (PCa). This clinical heterogeneity implies intra‐tumoural heterogeneity, with different prostate epithelial cell subtypes not all targeted by current treatments. We reported that such cell subtypes are traceable in liquid biopsies through representative transcripts. Expanding on this concept, we included 57 genes representing cell subtypes, drug targets and relevant to resistance as non‐invasive biomarkers for stratification. This panel was tested by RT‐qPCR (quantitative reverse transcription polymerase chain reaction) in blood of controls and different categories of PCa patients. Overall, circulating transcripts showed predictive value throughout the disease. Those with aggressive pathological features such as intra‐ductal carcinoma at diagnosis showed more genes over‐expressed. In metastatic patients, signatures of subtypes or resistance were associated with treatments, progression‐free survival and overall survival. Altogether, testing markers of cell diversity, an intrinsic feature of tumours, and drug targets via liquid biopsies represents a valuable means to stratify patients and predict responses to current or new therapeutic modalities. Over‐expressed drug target genes suggest potential benefit from targeted treatments, justifying new clinical trials to offer patient‐tailored strategies to eventually impact on PCa mortality.
No net loss accounting: Aligning biodiversity offsets with ecosystem accounts
Authors: Czúcz Bálint , Simensen Trond , Skrindo Astrid Brekke , Rusch Graciela M. , Nybø Signe , Grainger Matthew , Töpper Joachim Paul
Published: January 2025

Biodiversity offsetting and ecosystem accounting are two rapidly developing fields that share a common goal: quantifying changes in ecosystems. Nevertheless, the intersection of these fields is often overlooked, despite the significant synergies they offer. This perspective paper explores this intersection from both sides, highlighting the benefits of ecosystem accounting for offsetting practice and the steps needed to make ecosystem accounts offsetting‐ready.

The System of Environmental‐Economic Accounting—Ecosystem Accounting (SEEA EA) is the most widespread and sophisticated framework for ecosystem accounting. This framework was designed to consistently quantify biodiversity changes at several spatial scales, including fine scales typically relevant for biodiversity offsetting. Furthermore, the components of this ecosystem accounting framework are also tightly related to the key concepts of biodiversity offsetting. To illustrate this, we provide a dictionary cross‐linking the terminologies of the two fields.

Despite the fundamental similarities, most ecosystem accounts developed today are not (directly) suitable for fine‐scale use. We discuss the reasons for this, and the practical challenges of improving the suitability of ecosystem accounts for offsetting use.

Solution: Aligning ecosystem accounts and biodiversity offsetting offers a huge opportunity for both fields, enhancing the standardisation of offsetting practices, and making them extensible to high level no net loss biodiversity targets. This can be achieved by using offsetting‐relevant scalable biodiversity metrics as condition variables, and by implementing condition indices that yield meaningful offsetting currencies. We argue that future ecosystem accounting case studies should recognise offsetting and the quantification of net loss/gain as relevant use cases. And we also call for dedicated offsetting pilots that apply ecosystem accounts in concrete no net loss contexts, highlighting the transformative potential of harnessing these synergies for biodiversity policy.
Rational Design of Single‐Atom Nanozymes for Combination Cancer Immunotherapy
Authors: Lin Hanchao , Gao Yonghui , Zhu Le , Guo Yu , Zhang Lumin , Xie Jiali , Yang Dongqin , Liu Jing , Dong Qiongzhu , Zhu Zhiling
Published: January 2025

Remodeling of the tumor immune microenvironment and enhancement of antitumor immune responses are necessary to overcome immunotherapy resistance in tumors. However, tumor heterogeneity and complexity of immune evasion mechanisms pose significant therapeutic challenges. Nanozymes exhibit enzyme‐like characteristics and unique nanomaterial properties, showing potential for tumor therapy. However, design of effective nanozymes remains complex, inefficient, and functionally limited. Therefore, in this study, a novel strategy combining rationally designed single‐atom nanozymes (SAzymes) with immune checkpoint blockade (ICB) therapy is established. Molybdenum SAzymes supported on graphitic carbon nitride (Mo SAs) are constructed using 25 transition metal candidates from the 4th to 6th periods based on high‐throughput calculations and optimal piezoelectric‐enhanced multienzyme‐like activities. Upon activation by ultrasound, Mo SAs exerted potent therapeutic effects against ICB‐resistant tumors and remodeled the tumor immune microenvironment by inducing tumor immunogenic cell death, alleviating tumor hypoxia, and modulating chemokine expression in tumors. Combination of Mo SAs with anti‐programmed death protein‐1 antibodies further enhanced their antitumor efficacy, highlighting their potential to treat ICB‐resistant tumors.
Controlling of Circularly Polarized Luminescence via Modulating the Angle Between Transition Electric and Magnetic Dipole Moments
Authors: Yuan Li , Mao Dan , Yan Zhi‐Ping , Hu Jia‐Jun , Ni Hua‐Xiu , Hong Xian‐Fang , Zuo Jing‐Lin , Zheng You‐Xuan
Published: January 2025

The most challenging task in the development of chiral luminescent materials is currently increasing the dissymmetry factors (gPL, gEL) of circularly polarized luminescence (CPL). Current research primarily focuses on enhancing the molecular transition magnetic dipole moment, while the angle between the transition electric and magnetic dipole moment (θe,m) has received comparatively limited attention. In this study, two pairs of intrinsically axial chiral fluorescence (CP‐MR‐F) and thermally activated delayed fluorescence (CP‐MR‐TADF) enantiomers (R/S‐BBCz‐BN and R/S‐OBBCz‐BN) with increased g factors by modulating θe,m are reported. Based on R/S‐5H,5′H ‐6,6′‐bibenzo[b]carbazole units, the theoretically calculated θe,m and gcal for S‐BBCz‐BN are 17.7° and 1.1 × 10−2, respectively, enabling excellent CPL properties with |gPL| of 5.8 × 10−3 in toluene, representing one of the highest g values among MR materials. By employing the less conjugated R/S‐7,7′,8,8′,9,9′,10,10′‐octahydro‐5H,5′H‐6,6′‐bibenzo[b]carbazole groups with better electron donating ability, R/S‐OBBCz‐BN demonstrate good MR‐TADF and CPL properties with a peak at 467 nm, a full width at half‐maximum of 27 nm, and |gPL| of 2.5 × 10−3. Furthermore, the optimized circularly polarized organic light‐emitting diodes based on these two pairs of enantiomers showcase high maximum external quantum efficiencies of 24.7%/33.3% and mirror symmetrical circularly polarized electroluminescence with |gEL| factors of 3.4/1.2 × 10−3, respectively.
Nature Inspired Phototropic Artificial Photosynthesis
Authors: Zhang Jiaqi , Fang Tao , Chen Tingting , Yin Taishan , Zhao Yuqing , Cao Wenhan , Pang Huan , Huang Zhongjie
Published: January 2025

The efficiency of solar energy capture by terrestrial and solar device surfaces is significantly influenced by the variations in the solar angle of incidence, which change with latitude, season, and time of day. These fluctuations result in notable energy density losses. Photoelectrochemical (PEC) system‐based artificial leaf device has attracted immense research interests recently. However, its programmability and adaptiveness is highly desired and noticeably lacking. In this study, a novel programmable biomimetic PEC system—artificial aquatic plant—designed for bias‐free complete water splitting, capable of adapting is introduce to dynamic light incident angles. Inspired by key structures in aquatic plants, such as cytoplasm, chloroplasts, and petioles, this work incorporates innovative design with light‐weight PEC electrodes, protective hydrogel layers, integrated with light‐responsive hydrogel composites as supportive and actuating elements. As a result, this advanced device not only maintains stable complete water splitting performance but also exhibits characteristic phototropic properties, enhancing water splitting efficiency by 47% and 866% under light incident at 45° and 90°. Unlike traditional rigid systems, this work opens new avenues for the development of intelligent and programmable solar devices that can adapt to varying environments, paving the way for adaptive green energy technology and self‐sustaining energy production.
A Close Look at the Local Structure of Functional Polymers: The Example of Poly(Vinylidene Fluoride)
Authors: Kantrow Henry J. , Welton Claire , Thomas Neethu , Sarou‐Kanian Vincent , Pawlak Tomasz , Stingelin Natalie , Reddy G. N. Manjunatha
Published: January 2025

Functional macromolecular materials promise to enable important improvements in many aspects of everyday life, including for energy applications, novel electronics systems, or wearable health care products. In contrast to widely‐used commodity plastics such as polyolefins, polyamides, or polyesters, it, however, remains challenging to advance detailed insights into the interrelation of structure and performance for functional polymers, limiting progress. The reason is that the macroscopic properties of such polymers often depend on local chain arrangements rather than long‐range order only. Here, it is demonstrated on the example of the well‐investigated ferroelectric poly(vinylidene fluoride) (PVDF) that modern nuclear magnetic resonance (NMR) spectroscopy enables the quantitative analysis of the complex solid‐state structure of this polymer, providing unprecedented insight. The precise fractions of chain segments of different conformations are revealed, as well as their spatial distributions with respect to each other. Thereby, a significant population of short‐range ordered chain segments is identified, a large fraction of which in close proximity to defects and disordered segments. Unsurprisingly, different environments lead to different structural dynamics — collectively showing that characterizing local order/disorder and their dynamics is imperative to accurately describe the properties of functional polymers such as PVDF.
A Versatile Self‐Templating Approach for Constructing Ternary Halide Perovskite Heterojunctions to Achieve Concurrent Enhancement in Photocatalytic CO2 Reduction Activity and Stability
Authors: Zhang Meng‐Ran , Feng You‐Xiang , Liu Zhao‐Lei , Su Ke , Yuan Su‐Xian , Zhang Min , Lu Tong‐Bu
Published: January 2025

Metal halide perovskite (MHP)‐based photocatalysts encounter significant stability challenges in water‐containing systems, posing a major obstacle to their application in artificial photosynthesis. Herein, an innovative and universal strategy is present to create MHP‐based ternary heterojunctions based on a self‐templating method. A series of composite catalysts featuring sandwich hollow structures are constructed, with MHPs such as CsPbBr3, Cs3Bi2I9, Cs3Sb2Br9, and Cs2AgBiBr6 serving as the intermediate layers. The unique sandwich structure effectively shields MHPs from direct water contact, allowing MHP‐based photocatalysts to exhibit exceptional stability in water‐containing photocatalytic environments for durations exceeding 200 h. Furthermore, the hollow design ensures complete contact between the reaction substrates with both the oxidation and reduction functional areas. Compared to single perovskite materials, MHP‐based ternary heterojunction photocatalysts exhibit stronger oxidation capability and improved charge separation efficiency, leading to a substantial enhancement in photocatalytic CO2 reduction performance. Notably, the ternary heterojunction with CsPbBr3 as the intermediate layer achieves an electron consumption rate of up to 1824 µmol g−1 h−1 for CO2 reduction, which is far superior to other reported MHP‐based catalysts under similar conditions. This study provides a potent strategy for simultaneously enhancing the stability and activity of MHP‐based photocatalysts, paving the way for their potential applications in artificial photosynthesis.
Electrolyte Engineering with Asymmetric Spatial Shielding Effect for Aqueous Zinc Batteries
Authors: Cong Jianlong , Hu Zuyang , Hu Le , Li Tongjiang , Ji Haijin , Long Zihan , Fan Yuxin , Wen Zhipeng , Lin Yu‐Chang , Xu Henghui , Li Zhen , Li Shunning , Pan Feng , Huang Yunhui
Published: January 2025

The electrochemical instability of electrode/electrolyte interface and aqueous electrolyte collectively brings technical barriers, such as side reactions like hydrogen evolution and corrosion, as well as zinc dendrites, which hinder the practical application of aqueous zinc batteries. Here, an electrolyte engineering strategy is proposed with asymmetric spatial shielding effect by employing the molecules with asymmetric spatial structure as a cosolvent. Such molecule contains small methyl group and large cyclopentyl group to balance migration capability and shielding volume, which can not only promote the solvation structure of Zn2+ containing more anions and solid electrolyte interface derived from abundant anions but also rapidly and effectively adsorb on the surface of Zn anode to remodel the electric double layer. This strategy alleviates hydrogen evolution and corrosion while achieving dendrite‐free Zn deposition. Consequently, the Zn/I2 cell can operate stably at 2 A g−1 for 30 000 cycles over 180 days, with a capacity retention of 79.8%. Despite featuring a cathode areal capacity of 4.74 mAh cm−2 and an N/P ratio of 2.5, the Zn/NH4V4O10 cell still achieves an impressive capacity retention of 88.8% at 0.5 A g−1 for 200 cycles, demonstrating a significant potential for practical application.
A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants
Authors: Kim Se‐jeong , Guo Ning , Tan Zong Yao , Gao Xing , Fattahi Pouria , Liu Haijiao , Chang Jeehan , Younesi Mousa , Jung Selice , Chung Yewhan , Song Minkyung , Jung Michelle , Huh Dan Dongeun
Published: January 2025

Here a bioengineered platform is introduced to investigate adverse effects of environmental materials on the human cornea. Using primary cells, this system is capable of reproducing the differentiated corneal epithelium and its underlying stroma in the human eye, which can then be treated with externally applied solid, liquid, or gaseous substances in a controlled manner and under physiologically relevant conditions. The proof‐of‐principle of how this system can be used to simulate human ocular exposure to different classes of environmental toxicants for direct visualization and quantitative analysis of their potential to induce acute corneal injury and inflammation is demonstrated. This model can also be further engineered to create an electromechanically actuated array of multiple human corneal tissues that can emulate spontaneous eye blinking. Using this advanced system, it is shown that blinking‐like mechanical motions may play a protective role against adverse effects of environmental toxicants. This work yields an immediately deployable in vitro technology for screening ocular toxicity of existing and emerging environmental materials of various types and may enable the development of more realistic, human‐relevant preclinical toxicology models complementary to traditional animal testing.
Masquelet Inspired in Vivo Engineered Extracellular Matrix as Functional Periosteum for Bone Defect Repair and Reconstruction
Authors: Jiang Chen , Miao Tianfeng , Xing Xiaojie , Schilling Kevin J. , Lenhard Nicholas , Wang Lichen , McDowell Susan , Nilsson Bradley L. , Wang Hongjun , Zhang Xinping
Published: January 2025

The Masquelet technique that combines a foreign body reaction (FBR)‐induced vascularized tissue membrane with staged bone grafting for reconstruction of segmental bone defect has gained wide attention in Orthopedic surgery. The success of Masquelet hinges on its ability to promote formation of a “periosteum‐like” FBR‐induced membrane at the bone defect site. Inspired by Masquelet's technique, here a novel approach is devised to create periosteum mimetics from decellularized extracellular matrix (dECM), engineered in vivo through FBR, for reconstruction of segmental bone defects. The approach involved 3D printing of polylactic acid (PLA) template with desired pattern/architecture, followed by subcutaneous implantation of the template to form tissue, and depolymerization and decellularization to generate dECM with interconnected channels. The dECM matrices produces from the same mice (autologous) or from different mice (allogenic) are used as a functional periosteum for repair of structural bone allograft in a murine segmental bone defect model. This study shows that autologous dECM performed better than allogenic dECM, further permitting local delivery of low dose BMP‐2 to enhance allograft incorporation. The success of this current approach can establish a new line of versatile, patient‐specific, and periosteum‐like autologous dECM for bone regeneration, offering personalized therapeutics to patients with impaired healing.
Microenvironment Mechanical Torque from ZnFe2O4 (ZFO) Micromotors Inhibiting Tumor Migration
Authors: Jiang Tingting , Feng Ye , Gao Chao , Jiang Jiamiao , Chen Bin , Liu Suyi , Du Dailing , Ding Miaomiao , Rong Jinghui , Liao Zongzhen , Li Wensheng , Wilson Daniela A. , Tu Yingfeng , Song Shuqin , Wang Yi , Peng Fei
Published: January 2025

Mechanical force attracts booming attention with the potential to tune the tumor cell behavior, especially in cell migration. However, the current approach for introducing mechanical input is difficult to apply in vivo. How the mechanical force affects cell behavior in situ also remains unclear. In this work, an intelligent miniaturized platform is constructed with magnetic ZnFe2O4 (ZFO) micromotors. The wireless ZFO can self‐assemble in situ and rotate to generate mechanical torque of biologically relevant piconewton‐scale at the target tumor site. It is observed unexpectedly that enhanced in situ mechanical rotating torque from ZFO micromotors and the active fluid inhibit the migration of highly invasive A549 tumor cells. The down‐regulation of the Piezo1 channel and the suppressed signaling of ROCK1 in mechano‐adaptive tumor cells is found to be related to the inhibition effect. With effectiveness confirmed with the zebrafish xenograft model, this platform provides a valuable toolkit for mechanobiology and force‐associated non‐invasive tumor therapy.
Engineered Neutrophil Nanovesicles for Inhibiting Corneal Neovascularization by Synergistic Anti‐Inflammatory, Anti‐VEGF, and Chemoexcited Photodynamic Therapy
Authors: Liu Xiaoxue , Bi Yingxuan , Wei Chaoqun , Zhang Ye , Liu Xiaoyu , Guo Xinghan , Zhao Longfei , Zhang Jingjing , Wang Changlong , Gao Hua
Published: January 2025

Corneal neovascularization (CorNV) develops under various pathological conditions and is one of the main causes of blindness. Due to that CorNV progression involves multiple steps, anti‐vascular endothelial growth factor (VEGF) drugs alone could not sufficiently suppress this process, highlighting an urgent need for an efficient delivery system for the multi‐step management of CorNV. In this study, a neutrophil nanovesicle‐based eye drop (NCCR) is developed for CorNV therapy that simultaneously inhibits angiogenesis and inflammation, while eliminating pathological cells through chemoexcited photodynamic therapy (PDT). NCCR targets inflammatory lesions by leveraging the expression of chemokine receptors from the source cells. Then, NCCR exerts inhibitory effects on the sequential steps of neovascularization. First, it acts as a decoy and exerts an anti‐inflammatory effect by neutralizing cytokines via its receptors on the surface of nanovesicles. Second, thioketals bond‐linked ranibizumab is released in the high reactive oxygen species microenvironment of CorNV sites to bind VEGF, inhibiting vascular endothelial cell activation and proliferation. Finally, chemoexcited PDT eliminates preformed corneal blood vessels, disrupting tube formation and pericyte recruitment. The synergistic effects of NCCR on angiogenesis and inflammation, combined with the induction of apoptosis in neovessels via chemoexcited PDT, offer a novel and efficient strategy for CorNV treatment.
The First Molecular Ferroelectric Mott Insulator
Authors: Yao Jie , Sun Wencong , Guo Jianfeng , Feng Zi‐Jie , Pan Qiang , Peng Jin , Cheng Zhihai , Dong Shuai , Xiong Ren‐Gen , You Yu‐Meng
Published: January 2025

With the discovery of colossal magnetoresistance materials and high‐temperature superconductors, Mott insulators can potentially undergo a transition from insulating state to metallic state. Here, in molecular ferroelectrics system, a Mott insulator of (C7H14N)3V12O30 has been first synthesized, which is a 2D organic–inorganic ferroelectric with composition of layered vanadium oxide and quinuclidine ring. Interestingly, accompanied by the ferroelectric phase transition, (C7H14N)3V12O30 changes sharply in conductivity. The occurrence of a Mott transition has been proven by electric transport measurements and theoretical calculations. This research has significantly expanded the applicative horizons of ferroelectric materials, and offering an ideal platform for the investigation of strongly correlated electron systems.
Ultrafast Preparation of High‐Entropy NASICON Cathode Enables Stabilized Multielectron Redox and Wide‐Temperature (−50–60 °C) Workability in Sodium‐Ion Batteries
Authors: Du Miao , Li Kai , Yu Ning , Hao Ze‐Lin , Guo Jin‐Zhi , Liang Hao‐Jie , Gu Zhen‐Yi , Zhang Xiao‐Hua , Zhang Kai‐Yang , Liu Yan , Yang Jia‐Lin , Liu Yi‐Tong , Wu Xing‐Long
Published: January 2025

Avoiding severe structural distortion, irreversible phase transition, and realizing the stabilized multielectron redox are vital for promoting the development of high‐performance NASICON‐type cathode materials for sodium‐ion batteries (SIBs). Herein, a high‐entropy Na3.45V0.4Fe0.4Ti0.4Mn0.45Cr0.35(PO4)3 (HE‐Na3.45TMP) cathode material is prepared by ultrafast high‐temperature shock, which inhibits the possibility of phase separation and achieves reversible and stable multielectron transfer of 2.4/2.8 e− at voltage range of 2.0–4.45/1.5–4.45 V versus Na+/Na (the capacity of 137.2/162.0 mAh g−1). The galvanostatic charge/discharge and in‐situ X‐ray diffraction tests indicate the sequential redox reactions and approximate solid solution phase transition behavior of HE‐Na3.45TMP. Density functional theory calculations analyze the migration pathways and energy barriers, further confirming the superior reaction kinetics of HE‐Na3.45TMP. Accordingly, the HE‐Na3.45TMP exhibits outstanding wide temperature applicability and can operate stably in the temperature range of −50–60 °C, accompanied by a capacity retention of 92.8% after 400 cycles at −40 °C and a capacity of 73.7 mAh g−1 even at −50 °C. The assembled hard carbon//HE‐Na3.45TMP full‐cell offers an energy density of ≈301 Wh kg−1 based on total cathode and anode active mass, verifying the application feasibility of HE‐Na3.45TMP. This work provides an innovative and ultrafast pathway to rationally fabricate high‐performance cathodes for SIBs.
Self‐Supported Super‐Hydrophilic Interconnected Nanospikes and Particles of MoS2‐Ni3S2/NF with Optimum d‐Band Center for Anion Exchange Membrane Water Electrolyzer
Authors: Kumar Yogesh , Barik Sidharth , Samudre Nikhil S. , Kharabe Geeta Pandurang , Chauhan Inderjeet , Manna Narugopal , Bhat Suresh , Kurungot Sreekumar
Published: January 2025

There is an imperative need for highly efficient electrocatalysts for cost‐effective hydrogen production. Herein, a self‐supported, hybrid composite as a bifunctional electrocatalyst is introduced. This is achieved by in situ growth of MoS2‐Ni3S2 on nickel foam (NF), designated as MoS2‐Ni3S2/NF, synthesized by a facile one‐step hydrothermal synthesis method. MoS2‐Ni3S2/NF exhibits low overpotentials of only 187 and 146 mV for OER and HER, respectively, to achieve a current density of 10 mA cm−2 in 1 M KOH. The practical application of the designed bifunctional electrocatalyst is verified by constructing the MoS2‐Ni3S2/NF || MoS2‐Ni3S2/NF symmetrical membrane electrode assembly (MEA) of 4 cm2 working area for the anion exchange membrane water electrolyzer. The system shows continuous electrolysis for the monitored 48 h duration. For OER, an optimum d‐band center of −1.66 eV for the heterostructure is calculated from the Density Functional Theory (DFT) studies. The factors like the unique structure of the electrocatalyst, enhanced hydrophilicity, improved electrochemically accessible number of sites (ECASs), and optimum d‐band center, are expected to be the primary contributors to the system's improved performance. Thus, the present finding unveils a straightforward synthesis approach for creating a stable electrocatalyst for advancing commercial water electrolysis in the realm of renewable electrochemical energy conversion.
Nuclear Condensates of WW Domain‐Containing Adaptor With Coiled‐Coil Regulate Mitophagy via Alternative Splicing
Authors: Wang Jiahe , Fan Yi , Luo Guowen , Xiong Liang , Wang Lijie , Wu Zhuoxuan , Wang Jiayi , Peng Zhengying , Rosen Clifford J , Lu Kefeng , Jing Junjun , Yuan Quan , Zhang Zhenwei , Zhou Chenchen
Published: January 2025

Biomolecular condensates segregate nuclei into discrete regions, facilitating the execution of distinct biological functions. Here, it is identified that the WW domain containing adaptor with coiled‐coil (WAC) is localized to nuclear speckles via its WW domain and plays a pivotal role in regulating alternative splicing through the formation of biomolecular condensates via its C‐terminal coiled‐coil (CC) domain. WAC acts as a scaffold protein and facilitates the integration of RNA‐binding motif 12 (RBM12) into nuclear speckles, where RBM12 potentially interacts with the spliceosomal U5 small nuclear ribonucleoprotein (snRNP). Importantly, knockdown of RBM12, or deletion of the WAC CC domain led to altered splicing outcomes, resulting in an elevated level of BECN1‐S, the short splice variant of BECN1 that is shown to upregulate mitophagy. Thus, the findings reveal a previously unrecognized mechanism for the nuclear regulation of mitochondrial function through liquid–liquid phase separation (LLPS) and provide insights into the pathogenesis of WAC‐related disorders.
Improved Efficacy of Triple‐Negative Breast Cancer Immunotherapy via Hydrogel‐Based Co‐Delivery of CAR‐T Cells and Mitophagy Agonist
Authors: Li Guodong , Du Ruoxin , Wang Donghui , Zhang Xiangmei , Wang Lizhuo , Pu Shuangpeng , Li Xiaoju , Wang Shuning , Zhang Juliang , Liu Beichen , Gao Yuan , Zhao Huadong
Published: January 2025

Leaky and structurally abnormal blood vessels and increased pressure in the tumor interstitium reduce the infiltration of CAR‐T cells in solid tumors, including triple‐negative breast cancer (TNBC). Furthermore, high burden of tumor cells may cause reduction of infiltrating CAR‐T cells and their functional exhaustion. In this study, various effector‐to‐target (E:T) ratio experiments are established to model the treatment using CAR‐T cells in leukemia (high E:T ratio) and solid tumor (low E:T ratio). It is found that the antitumor immune response is decreased in solid tumors with low E:T ratio. Furthermore, single cell sequencing is performed to investigate the functional exhaustion at a low ratio. It is revealed that the inhibition of mitophagy‐mediated mitochondrial dysfunction diminished the antitumor efficacy of CAR‐T‐cell therapy. The mitophagy agonist BC1618 is screened via AI‐deep learning and cytokine detection, in vivo and in vitro studies revealed that BC1618 significantly strengthened the antitumor response of CAR‐T cells via improving mitophagy. Here, injection hydrogels are engineered for the controlled co‐delivery of CAR‐T cells and BC1618 that improves the treatment of TNBC. Local delivery of hydrogels creates an inflammatory and mitophagy‐enhanced microenvironment at the tumor site, which stimulates the CAR‐T cells proliferation, provides antitumor ability persistently, and improves the effect of treatment.
Peptide‐Drug Conjugate for Therapeutic Reprogramming of Tumor‐Associated Macrophages in Breast Cancer
Authors: Lepland Anni , Peranzoni Elisa , Haljasorg Uku , Asciutto Eliana K. , Crespí‐Amer Maria , Modesti Lorenzo , Kilk Kalle , Lombardia Manuel , Acosta Gerardo , Royo Miriam , Peterson Pärt , Marigo Ilaria , Teesalu Tambet , Scodeller Pablo
Published: January 2025

In triple‐negative breast cancer (TNBC), pro‐tumoral macrophages promote metastasis and suppress the immune response. To target these cells, a previously identified CD206 (mannose receptor)‐binding peptide, mUNO was engineered to enhance its affinity and proteolytic stability. The new rationally designed peptide, MACTIDE, includes a trypsin inhibitor loop, from the Sunflower Trypsin Inhibitor‐I. Binding studies to recombinant CD206 revealed a 15‐fold lower KD for MACTIDE compared to parental mUNO. Mass spectrometry further demonstrated a 5‐fold increase in MACTIDE's half‐life in tumor lysates compared to mUNO. Homing studies in TNBC‐bearing mice shows that fluorescein (FAM)‐MACTIDE precisely targeted CD206+ tumor‐associated macrophages (TAM) upon intravenous, intraperitoneal, and even oral administration, with minimal liver accumulation. MACTIDE was conjugated to Verteporfin, an FDA‐approved photosensitizer and YAP/TAZ pathway inhibitor to create the conjugate MACTIDE‐V. In the orthotopic 4T1 TNBC mouse model, non‐irradiated MACTIDE‐V‐treated mice exhibited anti‐tumoral effects comparable to those treated with irradiated MACTIDE‐V, with fewer signs of toxicity, prompting further investigation into the laser‐independent activity of the conjugate. In vitro studies using bone marrow‐derived mouse macrophages showed that MACTIDE‐V excluded YAP from the nucleus, increased phagocytic activity, and upregulated several genes associated with cytotoxic anti‐tumoral macrophages. In mouse models of TNBC, MACTIDE‐V slowed primary tumor growth, suppressed lung metastases, and increased markers of phagocytosis and antigen presentation in TAM and monocytes, increasing the tumor infiltration of several lymphocyte subsets. MACTIDE‐V is proposed as a promising peptide‐drug conjugate for modulating macrophage function in breast cancer immunotherapy.
Single Cell‐Pair Proteomics for Decoding Immune‐Cancer Cell Interactions
Authors: Xu Qin‐Qin , Jiang Yi‐Rong , Chen Jian‐Bo , Wu Jie , Chen Yi‐Xue , Fan Qian‐Xi , Wang Hui‐Feng , Yang Yi , Pan Jian‐Zhang , Fang Qun
Published: January 2025

The efficacy of cancer immunotherapy is significantly influenced by the heterogeneity of individual tumors and immune responses. To investigate this phenomenon, a microfluidic platform is constructed for profiling immune‐cancer cell interactions at the single‐cell proteomics level for the first time. Based on the platform, a comprehensive workflow is proposed for achieving accurate single‐cell pairing of an immune cell and a cancer cell with low cell damage and high success rate up to 95%, cell pair co‐culture, and real‐time microscopic monitoring of the cell‐pair interactions, cell pair retrieval, mass spectrometry‐based proteomic analysis of singe cell pairs, and decoupling of the proteomic information for each cell within the cell pair with the stable‐isotope labeling method. With the workflow, the interactions of single natural killer (NK) cells and single K562 tumor cells are investigated based on real‐time images and single cell‐pair proteomics. Notably, an identification depth of over 1000 protein groups in a single cell‐pair is achieved, leading to the discovery of sub‐clusters of NK cells with different functions and the identification of important biomarkers for cancer treatments. This demonstrates the unique capability of the present platform in providing substantial and comprehensive datasets for profiling immune‐cancer cell interactions, discovering heterogeneous immune responses, and predicting biomarkers in the study of cancer immunotherapy.