bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Myocardial Deformation Patterns and Prognostic Value of Routine Echocardiographic Parameters in Patients with Cardiac Sarcoidosis Versus Extracardiac Sarcoidosis: Systematic Review and Meta-Analysis
Authors: Jain Hritvik , Shahzad Maryam , Usman Muhammad , KC Anil , Singh Jagjot , Jain Jyoti , Odat Ramez M. , Goyal Aman , Ahmed Faizan , Ahmed Raheel
Published: February 2025

Background: Sarcoidosis is a multisystem disorder characterized by non-caseating granulomas in various organs. While cardiac sarcoidosis (CS) is clinically rare, it has significant implications, including heart failure, ventricular arrhythmias, and sudden cardiac death. Speckle-tracking echocardiography has emerged as a promising tool for detecting subclinical myocardial dysfunction, which is cost-efficient and readily available. This meta-analysis aims to evaluate differences in functional echocardiographic parameters between patients with CS and extracardiac sarcoidosis (ECS) to improve early recognition and management. Methods: A comprehensive search of major bibliographic databases was conducted to identify studies up to December 2024. Mean differences (MDs) with 95% CIs were pooled using the inverse-variance random-effect model. Results: Seven studies with 478 patients with sarcoidosis (CS: 159 and ECS: 319) were included. Patients with CS had a significant reduction in left ventricular global longitudinal strain (MD: −2.73; 95% CI: −4.09, −1.38; p < 0.0001) and tricuspid annular plane systolic excursion (MD: −0.59; 95% CI: −1.12, −0.05; p = 0.03) compared to patients with ECS. No significant differences in the LV global circumferential strain, interventricular septum thickness, left ventricular ejection fraction, E/A ratio, E/E’ ratio, LV end-diastolic diameter, and LV end-systolic diameter were noted. Conclusions: LV GLS and TAPSE are promising parameters for the early detection of cardiac involvement in sarcoidosis, with significant prognostic implications. Although STE provides a cost-effective and accessible alternative to CMR and FDG-PET, further research is needed to standardize its use and validate diagnostic cut-offs.
Precise Drought Threshold Monitoring in Winter Wheat Different Growth Periods Using a Multispectral Unmanned Aerial Vehicle
Authors: Song Wenlong , Liu Hongjie , Lu Yizhu , Lv Juan , Gui Rognjie , Chen Long , Li Mengyi , Chen Xiuhua
Published: February 2025

Agricultural drought significantly affects crop growth and food production, making accurate drought thresholds essential for effective monitoring and discrimination. This study aims to monitor the threshold ranges for different drought levels of winter wheat during three growth periods using a multispectral Unmanned Aerial Vehicle (UAV). Firstly, based on controlled field experiments, six vegetation indices were used to develop UAV optimal inversion models for the Leaf Area Index (LAI) and Soil–Plant Analysis Development (SPAD) during the jointing–heading period, heading–filling period, and filling–maturity period of winter wheat. The results show that during the three growth periods, the DVI-LAI, NDVI-LAI, and RVI-LAI models, along with the DVI-SPAD, RVI-SPAD, and TCARI-SPAD models, achieved the highest inversion accuracy. Based on the UAV-inversed LAI and SPAD indices, threshold ranges for different drought levels were determined for each period. The accuracy of LAI threshold monitoring during three periods was 92.8%, 93.6%, and 90.5%, respectively, with an overall accuracy of 92.4%. For the SPAD index, the threshold monitoring accuracy during three periods was 93.1%, 93.0%, and 92%, respectively, with an overall accuracy of 92.7%. Finally, combined with yield data, this study explores UAV-based drought disaster monitoring for winter wheat. This research enriches and expands the crop drought monitoring system using a multispectral UAV. The proposed drought threshold ranges can enhance the scientific and precise monitoring of crop drought, which is highly significant for agricultural management.
Goal Setting for Teacher Development: Enhancing Culturally Responsive, Inclusive, and Social Justice Pedagogy
Authors: Nganga Lydiah , Nydam Aaron , Kambutu John
Published: February 2025

This interpretive phenomenological study explores the perspectives of in-service, K-12 teachers in a graduate teacher education program on using goal setting to promote culturally responsive practices. The participants set two goals, documented their growth, and rated course instructional strategies that contributed to their learning. Data were collected through written reflections and responses to an anonymous midterm questionnaire in an online forum. Additional data came from the participants’ final reflections, the principal researcher’s reflective teaching notes, and end-of-semester reflections gathered by an unaffiliated critical friend after grades were posted. Coded data revealed that: 1. In-service teachers created goals that were relevant to specific areas of growth in professional learning and development in culturally responsive teaching practices and understanding diverse learners, 2. Goal setting, as a self-regulated learning strategy, serves to empower teachers toward teaching for promoting culturally responsive teaching practices, 3. Instructional supports and activities enhance personal development in becoming culturally responsive teachers, 4. Teacher educators’ reflection-on-action supports professional development.
Brain-Computer Interface Based Engagement Feedback in Virtual Reality Rehabilitation: Promoting Motor Cortex Activation
Authors: Lim Hyunmi , Ahmed Bilal , Ku Jeonghun
Published: February 2025

Maintaining optimal levels of engagement during rehabilitation training is crucial for inducing neuroplasticity in the motor cortex, which directly influences positive rehabilitation outcomes. In this research article, we propose a virtual reality (VR) rehabilitation system that incorporates a steady-state visual evoked potential (SSVEP) paradigm to provide engagement feedback. The system utilizes a flickering target and cursor to detect the user’s engagement levels during a target-tracking task. Eighteen healthy participants were recruited to experience three experimental conditions: no feedback (NoF), performance feedback (PF), and neurofeedback (NF). Our results reveal significantly greater Mu suppression in the NF condition compared to the other conditions. However, no significant differences were observed in performance metrics, such as tracking error, among the three conditions. The amount of feedback between the PF and NF conditions also showed no substantial difference. These findings suggest the efficacy of our SSVEP-based engagement feedback paradigm in stimulating motor cortex activity during rehabilitation. Consequently, we conclude that neurofeedback, based on the user’s attentional state, proves to be more effective in promoting motor cortex activation and facilitating neuroplastic changes. This research highlights the potential of integrating VR rehabilitation with an engagement feedback system for successful rehabilitation training.
MRB-YOLOv8: An Algorithm for Insulator Defect Detection
Authors: Xu Junhong , Zhao Shengjie , Li Yuan , Song Wenxin , Zhang Kecheng
Published: February 2025

As China’s electricity consumption surges, the reliability and safety of long-distance transmission lines become increasingly crucial. Insulators, vital for grid stability, demand accurate defect identification. Existing methods fall short on small targets and complex backgrounds. An insulator defect detection method MRB-YOLOv8 is proposed. By integrating an attention mechanism and multi-scale features, the model’s focus on key features is significantly improved. The Multi-Spectral Channel Attention captures essential information across different frequency domains through a well-designed frequency selection strategy. In addition, Receptive Field Attention Convolution (RFAConv) replaces the C2f module in the backbone network, which enhances the ability to perceive the features in complex backgrounds through the weighting operation of the receptive field weights. Meanwhile, the weighted bi-directional feature pyramid network (BiFPN) and a fourth detection layer prevent feature loss during fusion, enhancing the detection accuracy of small targets. Experimental results show that, at mAP50 and mAP50:95, the improved method obtains a gain of 3.2% and 3.6%, respectively, which significantly improves the model’s capability of detecting defects such as insulator self-explosion, breakage, and flashover in the images captured by UAVs.
ASOD: Attention-Based Salient Object Detector for Strip Steel Surface Defects
Authors: Yue Hongzhou , Li Xirui , Sun Yange , Zhang Li , Feng Yan , Guo Huaping
Published: February 2025

The accurate and efficient detection of steel surface defects remains challenging due to complex backgrounds, diverse defect types, and varying defect scales. The existing CNN-based methods often struggle with capturing long-range dependencies and handling complex background noise, resulting in suboptimal performance. Meanwhile, although Transformer-based approaches are effective in modeling global context, they typically require large-scale datasets and are computationally expensive, limiting their practicality for industrial applications. To address these challenges, we introduce a novel attention-based salient object detector, called the ASOD, to enhance the effectiveness of detectors for strip steel surface defects. In particular, we first design a novel channel-attention-based block including global max/average pooling to focus on the relevant channel-wise features while suppressing irrelevant channel responses, where maximizing pooling extracts the main features of local regions, while removing irrelevant features and average pooling obtain the overall features while removing local details. Then, a new block based on spatial attention is designed to emphasize the area with strip steel surface defects while suppressing irrelevant background areas. In addition, a new cross-spatial-attention-based block is designed to fuse the feature maps with multiple scales filtered through the proposed channel and spatial attention to produce features with better semantic and spatial information such that the detector adapts to strip steel defects of multiple sizes. The experiments show that the ASOD achieves superior performance across multiple evaluation metrics, with a weighted F-measure of 0.9559, an structure measure of 0.9230, a Pratt’s figure of meri of 0.0113, and an mean absolute error of 0.0144. In addition, the ASOD demonstrates strong robustness to noise interference, maintaining consistently high performance even with 10–20% dataset noise, which confirms its stability and reliability.
Optimizing Electric Vehicle Routing Efficiency Using K-Means Clustering and Genetic Algorithms
Authors: Gaon Tal , Gabay Yovel , Weiss Cohen Miri
Published: February 2025

Route planning for electric vehicles (EVs) is a critical challenge in sustainable transportation, as it directly addresses concerns about greenhouse gas emissions and energy efficiency. This study presents a novel approach that combines K-means clustering and GA optimization to create dynamic, real-world applicable routing solutions. This framework incorporates practical challenges, such as charging station queue lengths, which significantly influence travel time and energy consumption. Using K-means clustering, the methodology groups charging stations based on geographical proximity, allowing for optimal stop selection and minimizing unnecessary detours. GA optimization is used to refine these routes by evaluating key factors, including travel distance, queue dynamics, and time, to determine paths with the fewest charging stops while maintaining efficiency. By integrating these two techniques, the proposed framework achieves a balance between computational simplicity and adaptability to changing conditions. A series of experiments have demonstrated the framework’s ability to identify the shortest and least congested routes with strategically placed charging stops. The dynamic nature of the model ensures adaptability to evolving real-world scenarios, such as fluctuating queue lengths and travel demands. This research demonstrates the effectiveness of this approach for identifying the shortest, least congested routes with the most optimal charging stations, resulting in significant advancements in sustainable transportation and EV route optimization.
Eisenia bicyclis Extract Ameliorates Colitis in In Vitro and In Vivo Models Through Modulation of mTOR Axis and Gut Microbiota Composition
Authors: Wang Qunzhe , Im Yuri , Park Jumin , Lee Hye Lim , Ryu Dae Gon , Kim Hyemee
Published: February 2025

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon that is associated with dysbiosis in the gut microbiota. Eisenia bicyclis, a marine alga, is known for its anti-inflammatory, antioxidant, and gut microbiota-modulating properties. This study explored the mechanisms by which a 70% ethanol extract of E. bicyclis may alleviate UC, through both in vitro and in vivo experiments. LC-MS/MS analysis revealed eckol, 7-phloroeckol, dieckol, phlorofucofuroeckol A, and fucofuroeckol as key phenolic compounds present in the extract. The administration of E. bicyclis significantly improved symptoms in a dextran sulfate sodium (DSS)-induced colitis mouse model by reducing intestinal shortening, splenomegaly, and histological scores. Both cell and animal studies demonstrated that E. bicyclis suppressed the release of inflammatory cytokines, downregulated the mRNA expression of genes related to the mTOR pathway, and reduced the p-mTOR/mTOR ratio. Microbiota analysis revealed that, while the Firmicutes/Bacteroidetes ratio was elevated in UC mice, E. bicyclis administration normalized this imbalance, with a notable increase in the abundance of beneficial probiotics such as Bifidobacterium bifidum. In conclusion, a phenolic-rich extract of E. bicyclis demonstrates significant potential as a dietary supplement to prevent and mitigate UC by modulating both the mTOR signaling pathway and gut microbiota composition.
The Influence of Gold Nanoparticles Addition on Sugarcane Leaves-Derived Silica Xerogel Catalyst for the Production of Biodiesel
Authors: Maseko Ncamisile Nondumiso , Enke Dirk , Owolawi Pius Adewale , Iwarere Samuel Ayodele , Oluwafemi Oluwatobi Samuel , Pocock Jonathan
Published: February 2025

Biodiesel was produced via transesterification of canola oil in the presence of a silica xerogel catalyst with deposited gold nanoparticles. The silica-gold catalyst was produced in situ, where gold metal was added to a sodium silicate solution; subsequently, gold nanoparticles were synthesised within the solution. The sodium silicate-gold nanoparticles solution was then turned into a silica-gold gel at pH 8.7 and later dried to form silica-gold nanoparticles xerogel. The produced silica-gold nanoparticles xerogel was characterised by X-ray diffraction (XRD), X-ray fluorescence (XRF), transition electron microscopy (TEM), and nitrogen physisorption. The gel had a silica content of 91.6 wt% and a sodium content of 6.4 wt%, with the added gold content being 99.5% retained. The biodiesel produced in the presence of silica-gold nanoparticles xerogel was characterised by gas chromatography-mass spectroscopy (GC-MS) and its physical properties, such as density, kinematic viscosity, flash point, pour point, and cloud point, were also determined. The silica-gold nanoparticles xerogel catalyst remained solid throughout its usage without leaching into the reaction medium. The produced biodiesel contained mostly monounsaturated fatty acid methyl esters and had a yield of 99.2% at optimum reaction conditions.
Revealing the Importance of Iron Aerogel Features as Electrocatalysts for the Oxygen Reduction Reaction
Authors: González-Lavín Judith , Arenillas Ana , Rey-Raap Natalia
Published: February 2025

Metal nanoparticles supported in carbon materials are the traditional electrocatalyst currently used in many applications. However, these composite materials have many problems associated with the optimization of both components for the specific application, besides the stability of the mixture. Self-supported metallic materials may be an interesting strategy in order to avoid the traditional carbon supports; however, these metallic materials should present highly active surface area. Iron aerogels are presented in this work as effective and affordable unsupported electrocatalysts. The combination of their metallic structure with high porosity (i.e., 85 m2 g−1 and 0.45 cm3 g−1 of mesopore volume), due to their interconnected tridimensional structure, leads to a great activity versus the oxygen reduction reaction. A method for producing iron aerogels based on microwave-assisted sol–gel methodology is presented. The incorporation of carbon functionalities to the iron aerogels seems to clearly influence the mechanism of the reaction, favoring the direct mechanism of the oxygen reduction reaction and thus notably improving the performance of the electrocatalysts. Chemical vapor deposition seems to be an adequate methodology for incorporating carbon functionalities to the transition metal structure without affecting the tridimensional network and leading to current densities over 4 mA cm−2 and great stability even after 10,000 s.
Beyond Imitation: How Food Colloids Are Shaping the Next Generation of Biomimetic Foods
Authors: Guo Yong , Wang Jiacheng , Du Lianxin , Ma Chao , Xu Yan , Yang Xin
Published: February 2025

In the new global landscape of population, environmental, and energy sustainability, the manufacture of future food products that meet human nutritional and health needs is a major challenge. Biomimetic food, as a new type of food, has made significant progress in the use of plant proteins and other ingredients to mimic animal food, and it has also achieved important results in sensory and nutritional properties. In the study of biomimetic foods, food colloids play an irreplaceable role as the key framework for building food structures. In this paper, we first review the recent research progress on food colloids in the fields of biomimetic plant-based food, biomimetic animal-based food and 3D printed biomimetic food. Then, the mechanism of action, application effects, and quality improvement strategies of food colloids are deeply analyzed. Finally, the future research directions and application prospects are envisioned. This paper aims to give theoretical support and practical guidance for the development of biomimetic food through the above elaboration, to deal with the current problems in food development by means of the unique properties of food colloids, and to open up new ideas for the application of food colloids in future food innovation, and then to promote the further development of the field of biomimetic food.
Comparative and Phylogenetic Analysis of the Chloroplast Genomes of Four Wild Species of the Genus Prunus
Authors: Cui Mengfan , Liu Chenxi , Yang Xingling , Li Mingyu , Liu Liqiang , Jia Kai , Li Wenwen
Published: February 2025

Background: Prunus armeniaca, Prunus divaricata, Prunus tianshanica, and Prunus domestica are valuable ancient tree species that have persisted since the end of the Tertiary period within the Tianshan wild fruit forest. However, the evolutionary relationships among Prunus species in the Tianshan wild fruit forest have long posed a challenge. Methods: We sequenced and assembled the chloroplast genomes of P. armeniaca, P. divaricata, and P. tianshanica, and incorporated the chloroplast genome data of P. domestica for comparative analysis to elucidate their phylogenetic positions within the genus Prunus. Results: The lengths of these chloroplast genomes ranged from 157,395 bp to 158,090 bp, with a total of 130 to 131 genes annotated, comprising 85 to 86 protein-coding genes, 8 rRNA genes, and 35 to 36 tRNA genes. Notably, the four wild Prunus species predominantly used high-frequency codons ending in A/U. Additionally, we identified 300 simple repetitive sequences and 166 long repetitive sequences across the four wild Prunus species. The mutation sites were mainly found in the non-coding regions, with seven regions of high mutation frequency identified. The phylogenetic tree revealed five branches: subgenus Armeniaca, subgenus Microcerasus, subgenus Prunus, subgenus Amygdalus, and subgenus Cerasus. The estimated deviation time for the crown group of Prunus is roughly 61.41 million years ago. Conclusions: This study provides exhaustive genetic evidence for the classification and systematic relationships of the four wild Prunus species and establishes a crucial foundation for subsequent research into the diversity and evolutionary history of the Prunus genus.
Chromosome Morphology and Heterochromatin Patterns in Paspalum notatum: Insights into Polyploid Genome Structure
Authors: Honfi Ana I. , Reutemann A. Verena , Schneider Juan S. , Escobar Lucas M. , Martínez Eric J. , Daviña Julio R.
Published: February 2025

Background/Objectives: Paspalum notatum is a key multipurpose species native to American grasslands. This study provides, for the first time, a detailed karyotype analysis of diploid (2n = 2x = 20) and tetraploid (2n = 4x = 40) accessions of P. notatum, the most common cytotypes within the species. Methods: The constitutive heterochromatin patterns revealed using CMA-DA-DAPI staining and genome size estimations are novel contributions to the understanding of the N genome in Paspalum. Results: Chromosomes were small (1.1–2.3 µm), with the diploid karyotype comprising nine metacentric pairs (one bearing microsatellites on the short arms, pair 6) and one submetacentric pair. In tetraploids, the diploid karyotype was duplicated. Heterochromatin analysis revealed two CMA++/DAPI− bands located on the short arm and satellite of chromosome 6 in diploids, while tetraploids exhibited two to three CMA++/DAPI− and one to two CMA++/DAPI0 bands. The proportion of GC-rich heterochromatin represented 2.8 and 3.47% of the total chromosome length in diploid and tetraploid cytotypes, respectively. Genome size analysis revealed a reduction in monoploid genome size in tetraploids (1Cx = 0.678 pg) compared to diploids (1Cx = 0.71 pg), consistent with the autopolyploid origin hypothesis. Conclusions: These findings provide essential cytogenetic insights and suggest only minor structural changes in the N genome following polyploidization, which could guide future studies integrating genomic and cytogenetic maps of P. notatum.
Pre- and Postoperative Risk Factors for Hirschsprung-Associated Enterocolitis in Vietnamese Children
Authors: Viet Hoang Tran , Minh Tuan Huynh , Truong Nhan Vu , Phuong Anh Huynh Thi , Nguyen Bich-Uyen , The Hao Chung , Dang Cong Phi , Uy Linh Truong Nguyen
Published: February 2025

Background/Objective: Hirschsprung-associated enterocolitis (HAEC) can occur before and after surgery, increasing the complication rates, hospital stay, and treatment costs. This study aims to determine the incidence of preoperative and postoperative Hirschsprung-associated enterocolitis HAEC and the related risk factors. Methods: This study is a prospective cohort study of Hirschsprung’s disease patients under 16 years of age at two Children’s Hospitals in Ho Chi Minh City, Vietnam from December 2022 to June 2024. The postoperative follow-up is 12 months. Results: We enrolled 84 pediatric patients, with a male-to-female ratio of 5.4/1 and an average age of 7.2 ± 1.07 months. Of the patients, 25% had preoperative enterocolitis at the time of admission. Statistical analysis revealed that risk factors for preoperative enterocolitis at admission included a history of enterocolitis (p = 0.024), low weight in hospital (p = 0.001), and lack of preoperative treatment (p = 0.01). Postoperative enterocolitis occurred in 14 patients (16.7%) at an average of 4.03 months postoperatively. Multiple logistic regression of postoperative enterocolitis was associated with a history of preoperative enterocolitis (p < 0.001), anastomotic stricture (p = 0.002), and the length of the aganglionic segment (p = 0.031). No statistically significant association was found between the surgical method and the risk of postoperative enterocolitis. Conclusions: A history of preoperative enterocolitis, low weight, and anastomotic stricture are significant risk factors for postoperative enterocolitis in patients with Hirschsprung’s disease. Therefore, preoperative medical treatment is recommended for the patients who have not yet undergone a definitive surgical procedure.
“Lessons to Be Learned After the Storm”—A Retrospective Study on the Characteristics and Management of Dental Emergency Patients During the COVID-19 Outbreak in Riyadh
Authors: AlAqla Ali , Alrubaig Naif , Iyer Kiran , Alshareef Adeeb , Alkathiri Mohammed , Albassri Dana
Published: February 2025

Background/Objectives: There is a limited understanding of the variables relating to dental patients and the treatment provided during the initial phase of the COVID-19 lockdown in our region. This study aimed to qualitatively analyze these patient variables and determine the associations between treatment recommendations and the specialty of the doctor at the point of care. Methods: The present study was retrospective, cross-sectional, and analytical in nature. Data regarding symptoms, diagnosis, treatment, and the attending specialist were retrieved from the patient management software for patients seeking emergency dental services during the COVID-19 lockdown (23 March 2020 to 23 April 2020) in primary and tertiary public hospitals of the National Guard Health Affairs in Riyadh, Saudi Arabia. The association between exploratory (symptoms, diagnostic tool, specialist at point of care) and dependent variables (given diagnosis and treatment) was assessed using Fisher’s exact test and multinomial regression analysis. Results: A total of 151 dental patients attended the outpatient/emergency clinics during this period. The mean age of the patients in this study was 31.4 (±19.0) years. Compared to physicians, general dentists [OR 0.56, CI 0.29–10.47] were more likely to give an inappropriate diagnosis and treatment, whereas residents [OR 2.70, CI 1.65–98.17] and resident endodontists [OR 2.30, CI 1.28–78.11] were more likely to give an appropriate diagnosis and treatment. Conclusions: The findings of this study highlight the need for a greater number of endodontists at the forefront of screening and providing dental care during such health catastrophes.
Oral Health and Hygiene Practices from Baramulla District, Jammu and Kashmir, India: A Questionnaire-Based Cross-Sectional Observational Survey
Authors: Kango Hafsa , Jha Neelu Anand , Masoodi Parvaiz , Naz Aliya , Chowdhury Abhiroop
Published: February 2025

Background/Objectives: Oral hygiene practices are important for good oral health and overall well-being. In this study, we surveyed people across age groups and genders to understand the oral hygiene practices of Baramulla district of the Union Territory of Jammu and Kashmir. Methods: 488 participants—229 males and 259 females—were randomly interviewed through a structured 20-item questionnaire. A chi-square test was used to analyze the age and gender-specific associations in oral hygiene behaviors. Results: The study revealed that majority of participants brushed their teeth only once a day instead of the recommended twice a day; only young cohorts showed relatively higher practice of brushing twice daily. A significantly higher proportion of males (11.69%) rarely brushed their teeth compared to females (4.28%). Tongue cleaning was poorly reported across age and genders and more than 60% of respondents did not clean their tongue at all. Almost 75% and above had made dental visits at least once. However, the visits were made primarily in cases of pain and sensitivity, indicating negligence, financial issues, or a general lack of awareness. The younger cohorts reported eating fast food more frequently, indicating risk groups. The majority of male respondents (45.88%) reported fair oral health whereas the majority of female respondents (35.29%) reported poor overall oral health. Conclusions: Our results show that oral hygiene practices need more improvement, and the attitude and knowledge of the residents need to be ameliorated through oral health education policy interventions.
The Harnessing of Controlled Environment Agriculture Technologies for Phytochemical and Mineral Element Enrichment in Mesembryanthemum crystallinum
Authors: Samuolienė Giedrė , Pukalskas Audrius , Gudžinskaitė Ieva , Viršilė Akvilė
Published: February 2025

Controlled environment agriculture (CEA) facilitates the management of plant growth and development through innovative horticultural technologies. Specific features of obligatory halophytes require diverse cultivation conditions compared to leafy vegetables grown in CEA. An ice plant was grown in walk-in chambers, and the impact of the following aspects was evaluated: (I) photosynthetically active photon flux density (PPFD) of 150, 200, 250, 300 µmol m−2 s−1; (II) spectral composition of red (R), blue (B), RB, and RBFR (far-red); (III) 12 h, 16 h, and 24 h photoperiod; (IV) hydroponic solution pH at 5.0–5.5, 5.5–6.0, and 6.0–6.5; and (V) hydroponic solution salinity concentrations at 0, 50, 100, 150, and 200 mM L−1 NaCl. Other cultivation parameters were maintained constant. The results demonstrate that the ice plant was not sensitive to lighting duration at a constant daily light quantity. However, to enhance the phytochemical and mineral content per biomass, it is suggested that 250 µmol m−2 s−1 be used. While growth remains unaffected, pH levels 6.0–6.5 lead to an increased accumulation of fructose, citric, malic, and fumaric acids. In contrast, pH levels of 5.0–5.5 enhance the accumulation of mineral elements. Nutrient solution salinity of 50 mM NaCl is potentially optimum for ice plant growth. Further research is needed to evaluate the complex effect of environmental conditions for halophytes cultivation in CEA.
Identifying Plant Healthy Indicators of Five Tropical Perennials Using Certain Leaf Physiological Traits During Drought Stress and Re-Watering
Authors: He Jie , Goh Kai Jie , Qin Lin , Shen Yuanjie , Rahardjo Harianto
Published: February 2025

Perennials improve soil strength and stabilize the slope. However, they are very prone to drought stress (DS). To identify plant health indicators, this study investigated the responses of five tropical perennials commonly grown in Singapore’s slope to DS and re-watering (RW) in the greenhouse. The durations for mild, intermediate, and severe DS defined as T1, T2, and T3, respectively, before RW were based on the extents of reduced Fv/Fm ratio (maximal quantum efficiency of PSII) and the levels of wilting. After RW, soil water content (SWC) increased until field capacity in all DS soil, although they were significantly lower than in well-watered (WW) soil. Overall, the Fv/Fm ratios and leaf water content (LWC) decreased significantly in all DS plants compared to those of WW plants, but all increased to the similar level as WW plants after RW. Nitrogen deficiency did not occur in any plants during DS. There were clear positive correlations of SWC with Fv/Fm ratios, LWC, effective quantum yield of PSII (∆F/Fm’), electron transport rate (ETR), and photochemical quenching (qP) for all species. To monitor plant health, it would recommend using both non-destructive measurements such as SWC and Fv/Fm ratios and destructive parameters like LWC, ∆F/Fm’, ETR, and qP.
Luteolin Mitigates Photoaging Caused by UVA-Induced Fibroblast Senescence by Modulating Oxidative Stress Pathways
Authors: Yan Yu , Huang Haiting , Su Tongshan , Huang Wenyi , Wu Xinyu , Chen Xianxian , Ye Sen , Zhong Jun , Li Chun , Li Yu
Published: February 2025

As a polyphenolic plant flavone, luteolin (Lut) is widely found in many medicinal plants, flowers, and vegetables. Although Lut has been shown to have the effect of preventing and treating skin photoaging, its role in preventing photoaging specifically induced by ultraviolet A (UVA) radiation remains underreported. In vivo, BALB/c mice were used as models for skin photoaging models and treated with Lut. Additionally, NIH-3T3 fibroblasts were utilized in vitro to further investigate whether Lut exerts its anti-photoaging effects by enhancing fibroblast vitality and function. Several biochemical assays (CCK-8, catalase, superoxide dismutase, malondialdehyde, dichloro-dihydro-fluorescein diacetate, quantitative real-time-PCR, gene expression patterns) and histochemical (histological staining, immunofluorescent staining, SA-β-Gal experiments, western blotting analysis) were conducted. The findings demonstrate that the Lut pretreatment could enhance the vitality and function of fibroblasts in both in vitro and in vivo experiments and inhibit UVA-induced collagen degradation, thereby improving the skin’s resistance to photoaging. We confirmed that the Lut pretreatment inhibited the expression of UVA-induced senescent factors P21, P16, and pro-inflammatory senescence-associated secretory phenotype (SASP) factors. Additionally, Lut exhibited potent antioxidant effects during UVA exposure. Bioinformatics and network pharmacology analyses revealed that Lut’s anti-photoaging effects may be mediated through the regulation of oxidative stress-related pathways and anti-aging genes. Upon utilizing inhibitors and agonists of oxidative stress, we further confirmed that Lut prevents UVA-induced fibroblast senescence by suppressing oxidative stress, and ultimately protects the skin from photoaging damage. These findings indicate that lutein mitigates photoaging caused by UVA-induced fibroblast senescence through the modulation of oxidative stress pathways.
Correction of Significant Urethral Anomalies Using a Tissue-Engineered Human Urethral Substitute: Proof of Concept
Authors: Caneparo Christophe , Elia Elissa , Chabaud Stéphane , Berthod François , Fradette Julie , Bolduc Stéphane
Published: February 2025

Urethral reconstruction remains a challenge. Indeed, the use of oral mucosa, the reference biomaterial for urethroplasty, is associated with two main drawbacks: the limited availability of autologous tissues and potential short- and long-term complications, especially for patients with recurrences or severe anomalies. Therefore, the development of alternative approaches, such as urethral tissue engineering, is necessary. A new type of human urethral substitute devoid of exogenous biomaterials has been reconstructed in vitro. It presented sufficient mechanical strength and had histological and functional properties comparable to native tissues. These reconstructed tissues were implanted in vivo to repair hypospadias induced in tacrolimus-immunosuppressed rabbits via a two-stage urethroplasty. In the first stage, the distal part of the native urethra was removed, and a flat graft was implanted, leaving the urethra open proximally. Twelve weeks later, the graft was tubularized to create a neourethra, reproducing the usual clinical scenario. The results obtained for the experimental group were less effective than for the control group, with a success rate of 50% after excluding the animal affected by unwanted events unrelated to urethroplasty, and it is possible that the animal model or surgical technique used was not suitable and should be modified. Nevertheless, half of the urethral substitutes grafted on rabbits showed successful integration. These self-assembled artificial tissues represent promising substitutes for urethroplasty.
Therapeutic Education and Pharmacotherapeutic Follow-Up Protocol, a Useful Tool for the Improvement of Patients at Cardiovascular Risk in Community Pharmacies
Authors: Buenavida Jurado Pilar , De la Matta Martín Mª José , Martín Calero Mª José , De la Puerta Rocío
Published: February 2025

The aim was to determine the influence of a complex intervention based on pharmacotherapeutic follow-up (PTF) and the application of therapeutic education (TE) protocols on the clinical and educational parameters of patients at cardiovascular risk (CVR) attending community pharmacies (CPs). A prospective, longitudinal, randomized, controlled clinical trial was conducted over 6 months in patients from four Spanish CPs, divided into control (CG) and intervention (IG) groups. CG patients received usual pharmacy care and IG patients received a PTF- and TE-based intervention. The sample consisted of 85 elderly patients. After pharmaceutical follow-up of the IG patients, the following results were observed: significant reductions in cardiovascular risk (CVR) (p < 0.005), blood pressure (BP) (p < 0.05), and sedentary lifestyle (p < 0.001), as well as an improved knowledge of CVR and cardiovascular risk factors (CVRFs) (p < 0.001). Target values for BP were achieved in 27.2% of patients and for triglycerides in 12.4% of patients. The PTF of the patients showed that 29.2% did not have the expected response to some treatments, while 25% had untreated pathologies and 10% had adverse reactions. The TE protocols related to the patients’ educational needs, applied individually and in conjunction with the PTF, were able to improve their lifestyle habits, their knowledge of CVR, CVRFs, and pharmacotherapy, and their clinical parameters, and, thus, the level of development of their disease
Unhappy 20/20: A New Challenge for Cataract Surgery
Authors: De Gregorio Chiara , Nunziata Sebastiano , Spelta Sara , Lauretti Paolo , Barone Vincenzo , Surico Pier Luigi , Mori Tommaso , Coassin Marco , Di Zazzo Antonio
Published: February 2025

Background/Objectives: Cataract surgery has evolved into a refractive procedure aimed at optimizing both vision quality and quantity. Modern patients, particularly “baby boomers”, expect superior outcomes, increasing demand for premium intraocular lenses (IOLs). However, ocular surface dysfunction (OSD), especially dry eye disease (DED), compromises postoperative satisfaction, with up to 35% of patients dissatisfied despite achieving 20/20 visual acuity. This study aimed to characterize postsurgical ocular surface system failure (OSSF) and explore strategies to improve perioperative management. Methods: An open observational study was conducted at the Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy, enrolling 20 patients with stage N2–3 and C1–2 lens opacities. Patients with diabetes, prior surgeries, or ocular inflammatory diseases were excluded. Preoperative and postoperative assessments included OSDI, SANDE scores, Schirmer test, TBUT, and fluorescein staining. Follow-ups occurred at 1 week, 1 month, and 3 months postoperatively. Statistical analysis used two-way ANOVA (p < 0.05). Results: Despite achieving a BCVA of 20/20, 44% of patients reported OSSF symptoms. Postoperative evaluations revealed significant worsening in OSDI and SANDE scores (p < 0.001), Schirmer test (preoperative mean 19.92 ± 10.06; p < 0.001), and TBUT (preoperative mean 5.88 ± 2.64 s; p < 0.001). Meibomian gland dysfunction and conjunctival hyperemia also worsened. Conclusions: Postsurgical OSSF results from neurogenic inflammation, tear film instability, and meibomian gland dysfunction, exacerbated by surgical trauma. Preoperative and postoperative management, including artificial tears, lid hygiene, and preservative-free regimens, are essential to improve outcomes and patient satisfaction. Comprehensive strategies can mitigate symptoms and enhance the benefits of cataract surgery.
Current Advances in Optimal Medical Therapy for Heart Failure
Authors: Imamura Teruhiko
Published: February 2025

I am delighted to present this Special Issue, which focuses on the latest advancements in the optimal medical therapy for heart failure [...]
The Prevalence, Pathophysiological Role and Determinants of Mitral Annular Disjunction Among Patients with Mitral Valve Prolapse: A Systematic Review
Authors: Sonaglioni Andrea , Nicolosi Gian Luigi , Muti-Schünemann Giovanna Elsa Ute , Lombardo Michele , Muti Paola
Published: February 2025

Background: Over the last two decades, a number of imaging studies have evaluated the characteristics and clinical implications of mitral annular disjunction (MAD) among patients with mitral valve prolapse (MVP). The present systematic review has been primarily designed to summarize the main findings of these studies and to examine the overall impact of MAD in MVP patients. Methods: All imaging studies assessing the prevalence, pathophysiological role and determinants of MAD in MVP individuals, selected from the PubMed and EMBASE databases, were included. There was no limitation in terms of time period. The risk of bias was assessed by using the National Institutes of Health (NIH) Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies. Results: The full texts of 23 studies on 7718 MVP individuals were analyzed. The overall pooled prevalence of MAD in MVP individuals was 40% (range 5.4–90%). When considering the different imaging modalities for assessing MAD, the average MAD prevalence was 20% for cardiac computed tomography studies, 31.3% for transthoracic echocardiography (TTE) studies, 44.7% for transesophageal echocardiography studies and 47% for cardiac magnetic resonance studies. MAD presence was more commonly associated with female sex, young age, narrow antero-posterior thoracic diameter, symptoms of palpitations and syncope, T-wave inversion in inferolateral leads and frequent and/or complex ventricular arrhythmias (VAs) on electrocardiogram, myxomatous leaflets, bileaflet prolapse, larger mitral valve annulus and non-severe mitral regurgitation on TTE. A total of 12 studies (52.2%) provided follow-up data. Over a median follow-up time of 3.9 yrs (range 1–10.3 yrs), MVP individuals with MAD showed increased risk of clinical arrhythmic events, no difference in survival rate and good surgical outcomes. Conclusions: MAD was present in more than one-third of MVP patients, with a wide range of variability depending on the specific imaging method used for assessing MAD presence and on a nonunivocal MAD definition, with a possible overestimation due to Pseudo-MAD rather than True-MAD measurement. A multimodality imaging approach comprehensive of noninvasive chest shape assessment might improve MAD detection among MVP individuals. It appears that careful serial monitoring for VAs should be mandatory for MAD patients.
“Do You Hear What I Hear?” Speech and Voice Alterations in Hearing Loss: A Systematic Review
Authors: Di Stadio Arianna , Sossamon Jake , De Luca Pietro , Indovina Iole , Motta Giovanni , Ralli Massimo , Brenner Michael J. , Frohman Elliot M. , Plant Gordon T.
Published: February 2025

Background: Although hearing loss influences voice characteristics, such changes may be under-recognized during clinical consultations. This systematic review examines voice alterations in adults with post-lingual hearing loss, considering diagnostic and rehabilitative implications. Methods: A comprehensive search of PubMed, Scopus, and Google Scholar was conducted following PRISMA guidelines, targeting studies reporting quantitative data on vocal parameters in adults with sensorineural hearing loss. Exclusion criteria included pre-lingual hearing loss and non-English studies. Data extraction focused on pitch, loudness, and prosody, with study quality assessed using NIH tools. Results: Eleven case–control studies, involving 594 patients with sensorineural hearing loss and 326 control patients, were analyzed. Patients with untreated hearing loss exhibited elevated fundamental frequency, F0 (males: 158–169 Hz; females: 206–251 Hz) and loudness levels (males: 79–96 dB; females: 89–116 dB) compared to controls (F0—males: 75–150 Hz; females: 150–300 Hz; loudness—males: 30–70 dB; females: 40–68 dB). Alterations in jitter, shimmer, and maximum phonation time (MPT) contributed to the distinct “hearing loss voice”. Cochlear implants (CIs) and hearing aids improved vocal parameters, with CIs reducing F0 by approximately 12–15 Hz. Continuous hearing aid use normalized pitch and loudness within four months. Prosody alterations, such as monotone speech, were reported in long-term cases. In noisy environments, individuals with hearing loss exhibited exaggerated increases in pitch and loudness, indicative of compensatory mechanisms. Conclusions: Post-lingual hearing loss disrupts the central regulation of voice, altering pitch, loudness, and other vocal parameters. Recognizing these changes, particularly in noisy environments, could facilitate the early diagnosis and timely rehabilitation of hearing deficits, potentially mitigating associated risks of cognitive decline.
Application of Fractal Dimension Analysis of Sublingual Blood Vessel Patterns in Correlation with Cardiovascular Diseases—A Pilot Study Title
Authors: Janik Anastazja , Jurczyszyn Kamil , Wojtowicz Andrzej , Zieliński Fryderyk , Trafalski Mateusz
Published: February 2025

Background/Objectives: The blood vessel network can be used as a potential marker of general health. In the oral cavity, it is possible to diagnose systemic diseases as the manifestations of many metabolic, cardiovascular, hematological, and autoimmune diseases may represent in lingual tissue. Methods: In the present study, a fractal dimension analysis (FD) of blood vessels of the tongue was applied as a marker of the physiology as well as pathology of cardiovascular diseases. This study was conducted for old men and women aged 68–95 years. Intraoral photography of the lower surface of the tongue was analyzed and correlated with cardiovascular diseases. Results: Differences in the value of the fractal dimension of blood vessels, between hypertension and the control group, were found, as well as in heart coronary disease, atherosclerosis, and heart valve defects. Conclusions: FD changes in the microvascular network of the ventral surface of the tongue can be regarded as potential markers of certain systemic diseases. Based on them, diseases with a cardiovascular basis can be identified, which may expedite the diagnostic process for patients or serve the long-term monitoring of these conditions.
Fungal Empyema Thoracis Associated with Clavispora lusitaniae—First Report in a Domestic Cat
Authors: Garnoeva Radka , Dinkova Velina
Published: February 2025

Invasive fungal infections are life-threatening conditions that always pose a challenge to veterinary practitioners. The diagnostic and therapeutic approaches in a case of pleural effusion in a domestic cat with a 7-day history of progressive tachypnea were described. Fungal pyothorax was diagnosed on the basis of the clinical signs, radiography findings, complete blood counts, and isolation of the pathogen from pleural effusion samples on two occasions. After three thoracenteses for evacuation of the pleural exudate and 15-day therapy with terbinafine, the general condition of the patient was very good; the complete blood count and respiratory and heart rates returned to normal; and no diagnostic imaging signs of pleural effusion were present. To the best of our knowledge, this is the first report of empyema thoracis caused by Clavispora lusitaniae in a domestic cat. The described case emphasises the primary importance of timely identification of pathogenic agent(s) of feline pyothorax and appropriately prescribed treatment for the prevention of severe complications and fatal outcomes.