bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of a Swimming Training Season on Anthropometrics, Maturation, and Kinematics in 12-Year-Old and Under Age-Group Swimmers: A Network Analysis
Authors: Fiori Júlia Mello , Bandeira Paulo Felipe Ribeiro , Zacca Rodrigo , Castro Flávio Antônio de Souza
Published: February 2022

Understanding fluctuations and associations between swimming performance-related variables provide strategic insights into a swimmer's preparation program. Through network analysis, we verified the relationships between anthropometrics, maturation, and kinematics changes (Δ) in 25-m breaststroke (BREAST) and butterfly (FLY) swimming performance, before and after a 47-week swimming training season. Twenty age-group swimmers (n =11 girls: 10.0 ± 1.3 years and n = 9 boys: 10.5 ± 0.9 years) performed a 25-m all-out swim test (T25) in BREAST and FLY techniques, before and after 47 weeks. Three measures of centrality, transformed into a z-score, were generated: betweenness, closeness, and strength. Data were compared (t-test) and effect sizes were identified with Hedges' g. Large effect sizes were observed for swimming performance improvements in BREAST (32.0 ± 7.5 to 24.5 ± 3.8 s; g = 1.26; Δ = −21.9 %) and FLY (30.3 ± 7.0 to 21.8 ± 3.6 s; g = 1.52; Δ = −26.5 %). Small to moderate effect sizes were observed for anthropometric changes. Moderate effect size was observed for maturity offset changes (−2.0 ± 0.9 to −1.3 ± 1.0; g = 0.73; Δ = 50.9 ± 281 %). Changes in maturity offset, stroke rate (SR), and stroke length for both BREAST and FLY swimming speeds were highlighted by the weight matrix. For betweenness, closeness, and strength, changes in arm span (AS) (BREAST) and stroke length (FLY) were remarkable. The dynamic process of athletic development and the perception of complexity of fluctuations and associations between performance-related variables were underpinned, particularly for simultaneous swimming techniques in age-group swimmers.
Comparison of Clinical Features and Outcomes of Appendectomy in Elderly vs. Non-Elderly: A Systematic Review and Meta-Analysis
Authors: Yuan Jie , Chen Qingfeng , Hong Weicong , Yu Lifeng , Li Xuen
Published: February 2022

Background

The objective of this study is to compare clinical and surgical outcomes of appendectomy among elderly and non-elderly subjects.

Methods

A systematic search was conducted on PubMed, Scopus, and Google academic databases. Studies, observational in design, that compared peri-and postoperative outcomes of appendectomy, in patients with acute appendicitis, between elderly and non-elderly/younger subjects were considered for inclusion. Statistical analysis was performed using STATA software.

Results

A total of 15 studies were included. Compared to non-elderly patients, those that were elderly had an increased risk of complicated appendicitis [relative risk (RR), 2.38; 95% CI: 2.13, 2.66], peritonitis [RR, 1.88; 95% CI: 1.36, 2.59], and conversion from laparoscopic to open appendectomy [RR, 3.02; 95% CI: 2.31, 3.95]. The risk of overall postoperative complications [RR, 2.59; 95% CI: 2.19, 3.06], intra-abdominal abscess [RR, 1.84; 95% CI: 1.15, 2.96], wound infection [RR, 3.80; 95% CI: 2.57, 5.61], and use of postoperative drainage [RR, 1.14; 95% CI: 1.09, 1.19] was higher among the elderly. The risk of readmission (30 days) [RR, 1.61; 95% CI: 1.16, 2.24] and mortality (30 days) [RR, 12.48; 95% CI: 3.65, 42.7] was also higher among elderly.

Conclusions

Findings suggest an increased risk of peri-and postoperative complications among elderly subjects undergoing appendectomy, compared to non-elderly subjects.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42021286157.
Prevalence of Anisakid Nematodes in Fish in China: A Systematic Review and Meta-Analysis
Authors: Liu Qing , Wang Qi , Jiang Jing , Ma Jun-Yang , Zhu Xing-Quan , Gong Qing-Long
Published: February 2022

Anisakidosis, caused by anisakid larvae, is an important fish-borne zoonosis. This study aimed to summarize the prevalence of anisakid infection in fish in China. A systematic review and meta-analysis were performed using five bibliographic databases (PubMed, CNKI, ScienceDirect, WanFang, and VIP Chinese Journal Databases). A total of 40 articles related to anisakid infection in fish in China were finally included. Anisakid nematodes were prevalent in a wide range of fish species, and the overall pooled prevalence of anisakid nematodes in fish in China was 45.5%. Fresh fish had the highest prevalence rate (58.1%). The highest prevalence rate was observed in Eastern China (55.3%), and fish from East China Sea showed the highest prevalence of anisakid nematodes (76.8%). Subgroup analysis by sampling year suggested that the infection rate was higher during the years 2001–2011 (51.0%) than the other periods. Analysis of study quality revealed that the middle-quality studies reported the highest prevalence (59.9%). Compared with other seasons, winter had the highest prevalence (81.8%). The detection rate of anisakid nematodes in muscle was lower (7.8%, 95% CI: 0.0–37.6) than in other fish organs. Our findings suggested that anisakid infection was still common among fish in China. We recommend avoiding eating raw or undercooked fish. Region, site of infection, fish status and quality level were the main risk factors, and a continuous monitoring of anisakid infection in fish in China is needed.
Influence of Post Mortem Muscle Activity on Turkey Meat Quality
Authors: Leishman Emily M. , Vanderhout Ryley J. , van Staaveren Nienke , Barbut Shai , Mohr Jeff , Wood Benjamin J. , Baes Christine F.
Published: February 2022

Wing flapping and body movement can occur during the slaughter of poultry. Wing movement and flapping are driven primarily by the breast muscles (Pectoralis major and minor), and this muscle activity may have implications for meat quality. The objective of this study was to evaluate turkey post mortem activity during slaughter at a commercial poultry processing plant. Post mortem activity (during bleeding) was scored on 5,441 male turkeys, from six different genetic lines, using a 1–4 scale from none to severe wing flapping. Meat quality was measured on these birds in terms of pH (initial, ultimate, delta or change), color (L*, a*, b*), and physiochemical traits (drip loss, cooking loss, shear force). Linear mixed models were used to analyze the effect of activity (score 1–4), genetic line (A–F), and season (summer vs. autumn) on the nine meat quality traits. Post mortem activity influenced a*, drip loss, and shear force although the magnitude of the effects was small. There was an effect (P < 0.05) of genetic line on all the meat quality traits except for L*, cooking loss, and shear force. In general, larger, faster-growing lines had higher pH, but the relationship between the lines for the other traits is not as clear. Season affected all the meat quality traits, except for pHdelta, with meat having a higher pH, L*, b*, drip loss, cooking loss, and shear force in the summer. This study provides an exploratory assessment of post mortem activity in turkeys and identifies meat quality traits which are most affected while also accounting for the effects of genetic line and season. Although identified effect sizes are small, the cumulative effect on turkey meat quality may be more substantial.
Effects of Probiotics on the Growth Performance, Antioxidant Functions, Immune Responses, and Caecal Microbiota of Broilers Challenged by Lipopolysaccharide
Authors: Yu Yang , Li Qing , Zeng Xinfu , Xu Yinglei , Jin Kan , Liu Jinsong , Cao Guangtian
Published: February 2022

We aimed to study the effects of dietary Bacillus coagulans (B. coagulans) and Lactobacillus plantarum (L. plantarum) on broilers challenged by Escherichia coli lipopolysaccharide (LPS). One-day-old Cobb 500 chicks (360) were divided randomly into three treatment groups for 47 days: no supplementation (control, CON), B. coagulans supplementation (BC), and L. plantarum supplementation (LA). Broilers were routinely fed for 42 days and intraperitoneally injected with 500 μg LPS per kg body weight at 43, 45, and 47 days of age, respectively. Samples were collected 3 h after the last injection. At 1–21 days of age, the ADG in the BC and LA groups was higher than that in the CON group, and the feed to gain ratio (F/G) in the BC group was significantly decreased (P < 0.05). Compared with that in CON birds, the ADG was increased and the F/G was decreased in the BC and LA birds at 22–42 and 1–42 days of age, respectively (P < 0.05). After LPS stimulation, the endotoxin (ET), diamine oxidase (DAO), and D-lactic acid (D-LA) levels in the BC group were lower than those in the CON group (P < 0.05). The IgY, IgA, and IgM contents in the BC group and the IgY and IgM contents in the LA group were higher than those in the CON group (P < 0.05). The pro-inflammatory factor and interferon-β (IFN-β) contents (P < 0.05) decreased, and the anti-inflammatory factor content in the serum (P < 0.05) increased in the BC and LA groups. Compared with the CON and LA treatments, the BC treatment increased the concentrations of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), and decreased that of malondialdehyde (MDA) (P < 0.05). In contrast with the CON treatment, the BC and LA treatments increased the abundance of Ruminococcaceae and reduced that of Desulfovibrio (P < 0.05). Moreover, BC increased the abundance of beneficial bacteria. Overall, supplementation with B. coagulans and L. plantarum promoted the growth of broilers, improved their immunity and antioxidant capacity, and alleviated the LPS-stimulated inflammatory response by regulating the intestinal flora.
Somatostatin Receptor 2: A Potential Predictive Biomarker for Immune Checkpoint Inhibitor Treatment
Authors: Wang Aoyun , Yuan Yixiao , Chu Han , Gao Yixing , Jin Zheng , Jia Qingzhu , Zhu Bo
Published: February 2022

Somatostatin receptor 2 (SSTR2), the most abundant receptor of somatostatin (SST), possesses immunoreactivity and is altered in many cancers. However, the association between SSTR2 and efficacy of immune checkpoint inhibitors (ICIs) has not yet been reported. Immunohistochemistry (IHC) information across 20 cancers was collected from the Human Protein Atlas (HPA) and used to analyze the expression of SSTR2. Immune signatures collected from public databases, such as BioCarta or Reactome, were used to investigate the association between SSTR2 and the tumor microenviroment in the Cancer Genome Atlas (TCGA). Data from cohorts treated with ICIs were collected to assess whether SSTR2 is associated with benefits from ICIs treatment. In the HPA, we found the SSTR2 IHC-positive rate of 13 cancers to be above 50%. Five types of cancer express SSTR2 mildly (positive rate: 25%–50%), while the remaining two types of cancer barely stained SSTR2-positive (positive rate: 0%–24%). In TCGA analysis, immune cell signatures and immune function pathways were enriched in high SSTR2 expression groups in most cancers. In each ICIs treated cohort, patients with high SSTR2 expression experienced numerically superior objective response rate (Braun: 14.8% vs 13.4%, p = 0.85; Gide: 69.4% vs 40.5%, p = 0.025; Mariathasan: 22.4% vs 16.7%, p = 0.233; Miao: 37.5% vs 11.8%; Riaz: 32.0% vs 7.7%, p = 0.067) and overall survival (Braun: HR (95%CI): 0.80 [0.62–1.04], p = 0.80; Gide: HR (95%CI): 0.61 [0.29–1.30], p = 0.20; Mariathasan: HR (95%CI): 0.83 [0.64–1.08], p = 0.16; Miao: HR (95%CI): 0.24 [0.086–0.65], p = 0.0028; Nathanson cohort: HR (95%CI): 0 [0-inf], p = 0.18; Riaz: HR (95%CI): 0.24 [0.086–0.65], p = 0.028) than patients with low SSTR2 expression. In pooled cohort, we found these differences were significant (Pool: 24.6% vs 16.7%, p = 0.0077; HR (95% CI): 0.77 [0.65–0.91], p = 0.0018). Our results suggest that SSTR2 is a potential predictive biomarker for response to ICIs.
Adjacency Maps and Efficient Graph Algorithms
Authors: Valiente Gabriel
Published: February 2022

Graph algorithms that test adjacencies are usually implemented with an adjacency-matrix representation because the adjacency test takes constant time with adjacency matrices, but it takes linear time in the degree of the vertices with adjacency lists. In this article, we review the adjacency-map representation, which supports adjacency tests in constant expected time, and we show that graph algorithms run faster with adjacency maps than with adjacency lists by a small constant factor if they do not test adjacencies and by one or two orders of magnitude if they perform adjacency tests.
Simulation of Ultrasonic Backscattering in Polycrystalline Microstructures
Authors: Dobrovolskij Dascha , Schladitz Katja
Published: February 2022

Ultrasonic testing of polycrystalline media relies heavily on simulation of the expected signals in order to detect and correctly interpret deviations due to defects. Many effects disturb ultrasonic waves propagating in polycrystalline media. One of them is scattering due to the granular microstructure of the polycrystal. The thus arising so-called microstructural noise changes with grain size distribution and testing frequency. Here, a method for simulating this noise is introduced. We geometrically model the granular microstructure to determine its influence on the backscattered ultrasonic signal. To this end, we utilize Laguerre tessellations generated by random sphere packings dividing space into convex polytopes—the cells. The cells represent grains in a real polycrystal. Cells are characterized by their volume and act as single scatterers. We compute scattering coefficients cellwise by the Born approximation. We then combine the Generalized Point Source Superposition technique with the backscattered contributions resulting from the cell structure to compute the backscattered ultrasonic signal. Applying this new methodology, we compute the backscattered signals in a pulse-echo experiment for a coarse grain cubic crystallized Inconel-617 and a fine grain hexagonal crystallized titanium. Fitting random Laguerre tessellations to the observed grain structure allows for simulating within multiple realizations of the proposed model and thus to study the variation of the backscattered signal due to microstructural variation.
Pre-Flight Test Verification of Automatic Stabilization System Using Aircraft Trimming Surfaces
Authors: Zajdel Albert , Krawczyk Mariusz , Szczepański Cezary
Published: February 2022

The new requirement of installing the flight stabilization system onboard the airplanes for performing the single-pilot flights in IFR rules was issued lately. It caused the necessity of developing such a system for small aircraft. The proposed system was developed using Model-Based Design then tuned and tested in Model, Pilot and Hardware in the Loop Simulations. The paper presents the next advanced stage of testing—verification in simulation and ground tests on the PZL-130 Orlik airplane. The implementation of this system does not modify the pilot’s primary manual controls. The newly introduced electrical trim is used for automatic stabilization but can be used at manual trimming as it was previously, depending on the chosen operation mode. The ground tests were planned according to civil aviation authority and aviation law requirements. Chosen results from simulated flights were analyzed and presented, confirming the effectiveness of the proposed system. The dedicated application allowing the test engineer to change stabilization system parameters during the flight on a touchscreen tablet was developed and described. The outcome of the stabilization system test campaign was a verification of its performance before the flight tests. The comparison of simulated and real flight data will allow identifying model deficiencies and flight stabilization system efficiency, which makes possible improvements implementation. Additionally, it appeared to be the cost-effective and less electrical energy-consuming automatic flight stabilization system for small aircraft. Such features benefit initiatives like Future Sky, More Electric Aircraft and aircraft stabilization system retrofit.
Design and Simulation Analysis of an Electromagnetic Damper for Reducing Shimmy in Electrically Actuated Nose Wheel Steering Systems
Authors: She Chenfei , Zhang Ming , Ge Yibo , Tang Liming , Yin Haifeng , Peng Gang
Published: February 2022

Based on the technical platform of electrically actuated nose wheel steering systems, a new type of damping shimmy reduction technology is developed to break through the limitations of traditional hydraulic damping shimmy reduction methods, and an electrically actuated nose wheel steering structure scheme is proposed. The mathematical model of the electromagnetic damper is established, the derivation of skin depth, damping torque and damping coefficient is completed, and the design of the shape and size of the electromagnetic damper is combined with the derivation results and the technical index of shimmy reduction. The electromagnetic field finite element simulation results show that the mathematical modeling method of the electromagnetic damper has good accuracy, and its application to the shimmy reduction module of the electrically actuated nose wheel steering system is also feasible and superior. Finally, the key factors influencing the performance of electromagnetic damper shimmy reduction are studied and analyzed, thus forming a complete electromagnetic damper shimmy reduction technology for the electrically actuated system, and laying the foundation for the design of novel all-electric aircraft and landing gear.
Combination of Limited Meteorological Data for Predicting Reference Crop Evapotranspiration Using Artificial Neural Network Method
Authors: Elbeltagi Ahmed , Nagy Attila , Mohammed Safwan , Pande Chaitanya B. , Kumar Manish , Bhat Shakeel Ahmad , Zsembeli József , Huzsvai László , Tamás János , Kovács Elza , Harsányi Endre , Juhász Csaba
Published: February 2022

Reference crop evapotranspiration (ETo) is an important component of the hydrological cycle that is used for water resource planning, irrigation, and agricultural management, as well as in other hydrological processes. The aim of this study was to estimate the ETo based on limited meteorological data using an artificial neural network (ANN) method. The daily data of minimum temperature (Tmin), maximum temperature (Tmax), mean temperature (Tmean), solar radiation (SR), humidity (H), wind speed (WS), sunshine hours (Ssh), maximum global radiation (gradmax), minimum global radiation (gradmin), day length, and ETo data were obtained over the long-term period from 1969 to 2019. The analysed data were divided into two parts from 1969 to 2007 and from 2008 to 2019 for model training and testing, respectively. The optimal ANN for forecasting ETo included Tmax, Tmin, H, and SR at hidden layers (4, 3); gradmin, SR, and WS at (6, 4); SR, day length, Ssh, and Tmean at (3, 2); all collected parameters at hidden layer (5, 4). The results showed different alternative methods for estimation of ETo in case of a lack of climate data with high performance. Models using ANN can help promote the decision-making for water managers, designers, and development planners.
Effects of Short-Term Tillage Managements on CH4 and N2O Emissions from a Double-Cropping Rice Field in Southern of China
Authors: Tang Haiming , Li Chao , Shi Lihong , Cheng Kaikai , Wen Li , Li Weiyan , Xiao Xiaoping
Published: February 2022

Soil carbon (C) content plays an important role in maintaining or increasing soil quality and soil fertility. However, the impacts of different tillage and crop residue incorporation managements on greenhouse gas (GHG) emissions from paddy fields under the double-cropping rice (Oryza sativa L.) system in southern China still need further study. Therefore, a field experiment was conducted to determine the impacts of different short-term (5-years) tillage and crop residue incorporation managements on soil organic carbon (SOC) content, SOC stock, and GHG emissions from paddy fields under the double-cropping rice system in southern China. The field experiment included four tillage treatments: rotary tillage with all crop residues removed as a control (RTO), conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), and no-tillage with crop residue retention (NT). These results indicated that SOC stock in paddy fields with CT, RT, and NT treatments increased by 4.64, 3.60, 3.50 Mg ha−1 and 4.68, 4.21, and 4.04 Mg ha−1 in 2019 and 2020, respectively, compared with RTO treatment. The results showed that early rice and late rice yield with CT treatment increased by 7.22% and 19.99% in 2019 and 6.19% and 6.40% in 2020, respectively, compared with RTO treatment. A two-year (2019–2020) investigation of GHG results indicated that methane emissions from paddy fields with NT treatment were decreased, but nitrous oxide emissions from paddy fields were increased. The lowest mean global warming potential (GWP) and per yield GWP carbon dioxide were found with NT treatment, compared to RT and CT treatments. Therefore, it was a beneficial practice for maintaining SOC stock and decreasing GHG mitigation under the double-cropping rice system in southern China by applying no-tillage with crop residue retention management.
The Essential-Oil-Bearing Rose Collection Variability Study in Terms of Biochemical Parameters
Authors: Zolotilov Viktor , Nevkrytaya Natalya , Zolotilova Olga , Seitadzhieva Sevilia , Myagkikh Elena , Pashtetskiy Vladimir , Karpukhin Mikhail
Published: February 2022

The primary task when breeding new varieties of essential-oil-bearing rose is to increase the essential oil content and quality. The purpose of the present research is to study the essential-oil-bearing rose collection variability in terms of the essential oil content and component composition and to identify opportunities for isolating the specimens promising for selection. The study of a collection of 112 specimens was carried out in 2017–2020 in the context of the piedmont of Crimea. The decanted essential oil content was determined using the hydrodistillation method. The component composition of rose essential oil was analyzed by gas chromatography on Crystal 5000.2. The essential oil components were identified by comparing their Kovats retention indices to the literature values. A high variability in the essential oil content in the collection (Cv = 36.3% at the average, over 4 years) and the major components content in the essential oil (Cv = 22.1–45.9%) was found. In the context of the piedmont of Crimea, the major components’ percentage content in essential oil from all the specimens including the five Bulgarian varieties did not meet the GOST ISO 9842-2017 standard requirements. This is indicative of a high-degree sensitivity to the soil and weather conditions in the region. It was found that the citronellol, geraniol, and nerol content in essential oil dropped significantly in extreme high temperature and drought conditions. Seven specimens rich in essential oil contained in the raw plant material (0.030–0.049%) and thirteen specimens with a high yield of concrete (0.31–0.39%) were identified and have been recommended for inclusion in the breeding process.
Milk Supplemented with Organic Iron Improves Performance, Blood Hematology, Iron Metabolism Parameters, Biochemical and Immunological Parameters in Suckling Dalagh Lambs
Authors: Asadi Mohammad , Toghdory Abdolhakim , Hatami Maryam , Ghassemi Nejad Jalil
Published: February 2022

This study was conducted to investigate the effect of milk supplemented with organic iron on performance, blood hematology, iron metabolism parameters, biochemical and immunological parameters in suckling lambs. Thirty-six newborn Dalagh lambs were randomly divided into three groups with 12 replications. The control group was fed with milk without organic iron. The other two groups were fed milk supplemented with 25 and 50 mg/d organic iron, respectively. During the experiment, increased daily weight gain and total body weight were observed in the iron-supplemented groups. An increase in the levels of red blood cell, hemoglobin, hematocrit, mean corpuscular hemoglobin, and mean corpuscular concentration in iron supplemented groups was indicated. Consumption of organic iron caused a significant decrease in plasma copper concentration. Total antioxidant status level was lower, but levels of glutathione peroxidase, superoxide dismutase, and catalase were higher in iron supplemented groups. In organic iron supplemented groups, insulin and thyroid hormones levels were significantly increased, and glucose level was significantly decreased. In organic iron supplemented groups, alkaline phosphatase level significantly increased, and aminotransferase level significantly decreased. Overall, the use of organic iron in the milk improved the performance and health in suckling lambs, and since a lower level of supplementation is naturally preferable, supplementation of milk with 25 mg/d organic iron is recommended.
The Effect of Environmental Enrichment on Laboratory Rare Minnows (Gobiocypris rarus): Growth, Physiology, and Behavior
Authors: Xu Chunsen , Hou Miaomiao , Su Liangxia , Qiu Ning , Yu Fandong , Zou Xinhua , Wang Chunling , Wang Jianwei , He Yongfeng
Published: February 2022

Environmental enrichment is a method to increase environmental heterogeneity, which may reduce stress and improve animal welfare. Previous studies have shown that environmental enrichment can increase the growth rate, decrease aggressive and anxiety-like behaviors, improve learning ability and agility, and reduce cortisol levels in animals. These effects usually differ between species. Unfortunately, habitat enrichment on laboratory fish is poorly studied and seldom adopted in care guidance. Rare minnows (Gobiocypris rarus) have been cultured as a native laboratory fish in China in barren banks without environmental enrichment since 1990; they have been widely used in studies on ecotoxicology, environmental science, and other topics. The purpose of this study was to investigate the effect of environment enrichment on the growth, physiological status, and anxiety-like behavior of laboratory rare minnows. We observed and analyzed SGR, cortisol levels, DA, DOPAC, 5-HT and 5-HIAA, and anxiety-like behavior indexes after one month of treatment in barren (control) and enrichment tanks. We found that there were no significant differences in SGR, anxiety-like behavior, DA, DOPAC, or 5-HIAA levels between the two treatments. However, higher cortisol and 5-HT levels were observed in the enrichment tanks. This study suggests that rare minnows might be influenced by their living environment, and future related studies should consider their environmental enrichment.
The Shape of the Nasal Cavity and Adaptations to Sniffing in the Dog (Canis familiaris) Compared to Other Domesticated Mammals: A Review Article
Authors: Buzek Anna , Serwańska-Leja Katarzyna , Zaworska-Zakrzewska Anita , Kasprowicz-Potocka Małgorzata
Published: February 2022

Dogs are a good starting point for the description and anatomical analysis of turbinates of the nose. This work aimed at summing up the state of knowledge on the shape of the nasal cavity and airflow in these domestic animals and dealt with the brachycephalic syndrome (BOAS) and anatomical changes in the initial airway area in dogs with a short and widened skull. As a result of artificial selection and breeding concepts, the dog population grew very quickly. Modern dog breeds are characterized by a great variety of their anatomical shape. Craniological changes also had a significant impact on the structure and physiology of the respiratory system in mammals. The shape of the nasal cavity is particularly distinctive in dogs. Numerous studies have established that dogs and their olfactory ability are of great importance in searching for lost people, detecting explosives or drugs as well as signaling disease in the human body. The manuscript describes the structure of the initial part of the respiratory system, including the nasal turbinates, and compares representatives of various animal species. It provides information on the anatomy of brachycephalic dogs and BOAS. The studies suggest that further characterization and studies of nasal turbinates and their hypertrophy are important.
Microbiological Diagnoses on Clinical Mastitis—Comparison between Diagnoses Made in Veterinary Clinics versus in Laboratory Applying MALDI-TOF MS
Authors: Astrup Lærke Boye , Pedersen Karl , Farre Michael
Published: February 2022

The present study compares the diagnoses on clinical bovine mastitis made in veterinary clinics using conventional diagnostic methods with diagnoses on the same samples made by a veterinary reference laboratory using MALDI-TOF MS as diagnostics. The study enables targeted and evidence-based consulting on prudent mastitis diagnostics and related antibiotic usage. In total, 492 samples from clinical mastitis were included. When applying MALDI-TOF MS as gold standard, only 90 out of 492 diagnoses made in veterinary clinics, equal to 18%, were correct. Four main findings were important: (1) the veterinary clinics overlooked contamination in mastitis samples; (2) the veterinary clinics only assigned 2 fully correct diagnoses out of 119 samples with mixed growth cultures; (3) the veterinary clinics made close to half of their diagnoses on pure culture erroneously; (4) the veterinary clinics applied a limited number of the relevant pathogen identifications on pure culture samples. Altogether, the present study shows that a large part of Danish clinical mastitis cases are misdiagnosed. Lack of correct diagnoses and diagnostic quality control may lead to the choice of wrong treatment and thus hamper prudent use of antibiotics. Hence, the present study warns a risk of overuse of antibiotics in Denmark. Consequently, the present study calls for training of veterinary clinics in diagnostics of mastitis pathogens and national guidelines on quality assurance of mastitis diagnostics.
Stapling of Peptides Potentiates the Antibiotic Treatment of Acinetobacter baumannii In Vivo
Authors: Schouten Gina , Paulussen Felix , Kuipers Oscar , Bitter Wilbert , Grossmann Tom , van Ulsen Peter
Published: February 2022

The rising incidence of multidrug resistance in Gram-negative bacteria underlines the urgency for novel treatment options. One promising new approach is the synergistic combination of antibiotics with antimicrobial peptides. However, the use of such peptides is not straightforward; they are often sensitive to proteolytic degradation, which greatly limits their clinical potential. One approach to increase stability is to apply a hydrocarbon staple to the antimicrobial peptide, thereby fixing them in an α-helical conformation, which renders them less exposed to proteolytic activity. In this work we applied several different hydrocarbon staples to two previously described peptides shown to act on the outer membrane, L6 and L8, and tested their activity in a zebrafish embryo infection model using a clinical isolate of Acinetobacter baumannii as a pathogen. We show that the introduction of such a hydrocarbon staple to the peptide L8 improves its in vivo potentiating activity on antibiotic treatment, without increasing its in vivo antimicrobial activity, toxicity or hemolytic activity.
Can Antioxidants Reduce the Toxicity of Bisphenol?
Authors: Mączka Wanda , Grabarczyk Małgorzata , Wińska Katarzyna
Published: February 2022

BPA is still the subject of extensive research due to its widespread use, despite its significant toxicity resulting not only from its negative impact on the endocrine system but also from disrupting the organism’s oxidative homeostasis. At the molecular level, bisphenol A (BPA) causes an increased production of ROS and hence a change in the redox balance, mitochondrial dysfunction, and modulation of cell signaling pathways. Importantly, these changes accumulate in animals and humans, and BPA toxicity may be aggravated by poor diet, metabolic disorders, and coexisting diseases. Accordingly, approaches using antioxidants to counteract the negative effects of BPA are being considered. The preliminary results that are described in this paper are promising, however, it should be emphasized that further studies are required to determine the optimal dosage and treatment regimen to counteract BPA toxicity. It also seems necessary to have a more holistic approach showing, on the one hand, the influence of BPA on the overall human metabolism and, on the other hand, the influence of antioxidants in doses that are acceptable with the diet on BPA toxicity. This is due in part to the fact that in many cases, the positive effect of antioxidants in in vitro studies is not confirmed by clinical studies. For this reason, further research into the molecular mechanisms of BPA activity is also recommended.
Effect of Dietary Grapes on Female C57BL6/J Mice Consuming a High-Fat Diet: Behavioral and Genetic Changes
Authors: Parande Falguni , Dave Asim , Park Eun-Jung , McAllister Christopher , Pezzuto John M.
Published: February 2022

(1) Background: Adverse effects of a chronic high-fat diet (HFD) on murine behavior, cognition, and memory are well established. Polyphenols such as resveratrol, anthocyanins, and flavonoids, that are known for antioxidative and anti-inflammatory properties, are present in grapes. The objective of this work was to determine if the dietary intake of grapes has the potential of alleviating HFD-induced deficiencies. (2) Methods: The effect of dietary grape intake was studied using behavioral assays and high throughput genome-wide RNA transcriptome analyses with female C57BL6/J mice. (3) Results: Mice that were fed a HFD from 3-weeks of age showed anxiety-like behaviors compared with the standard diet (STD). This HFD-induced effect was attenuated by supplementing the HFD with 1% grape powder (HF1G) (open field test). Similar results were observed with the novel object recognition test; there was a significant difference in time spent exploring a novel object between the HFD and the HF1G groups. There was no significant difference between the HFD1G and the STD groups. Based on the RNA-Seq analysis, genetic expression in the brain varied as a result of diet, with 210, 360, and 221 uniquely expressed genes in the STD, HFD, and HF1G groups, respectively. Cluster analysis revealed that the HFIG group mapped more closely with the STD group than the HFD group. Focusing on some specific areas, based on genetic expression, Dopamine receptor 2 (Drd2) was increased in the HFD group and normalized in the HF1G group, relative to the STD group. In addition, as judged by cluster hierarchy, the expression of genes that are associated with the dopamine receptor 2 pathway were increased in the HFD group, whereas the pattern that was derived from mouse brain from the HF1G group showed greater similarity to the STD group. KEGG pathway analyses were consistent with these results. For example, neuroactive ligand-receptor interaction (KEGG ID: mmu04080) was altered due to HFD compared with STD, but normalized by grape supplementation or the HFD; there was no significant difference between the STD and HF1G groups. In addition, the expression of genes related to feeding behavior, such as Adora2a, Th, and Trh, were also increased in the HFD group compared with the STD group, and attenuated by grape supplementation. (4) Conclusions: Dietary grape consumption has positive effects on behavior and cognition that are impaired by a HFD. Attenuation of these effects correlates with global transcriptional changes in mouse brain.
Effect of Crushing Peanuts on Fatty Acid and Phenolic Bioaccessibility: A Long-Term Study
Authors: Parilli-Moser Isabella , Domínguez-López Inés , Arancibia-Riveros Camila , Marhuenda-Muñoz María , Vallverdú-Queralt Anna , Hurtado-Barroso Sara , Lamuela-Raventós Rosa M.
Published: February 2022

Background: Peanuts are consumed worldwide and have been linked to multiple health benefits. Processing may affect the bioavailability of peanut bioactive compounds. Therefore, we aim to evaluate the effects of crushing peanuts on the bioavailability of fatty acids and phenolic compounds in healthy adults. Methods: 44 participants from the ARISTOTLE study consumed 25 g/day of whole peanuts (WP) or 32 g/day of peanut butter (PB) for 6 months. Fatty acids and phenolic compounds in peanut products and biological samples were assessed by gas chromatography coupled to flame ionization detection and liquid chromatography coupled to high resolution mass spectrometry, respectively. Results: Plasma concentrations of very long chain saturated fatty acids (VLCSFAs) increased significantly after 6 months of WP or PB intake (p < 0.001 in both cases). Participants in the WP group excreted twice as many VLCSFAs in feces as those in the PB group (p = 0.012). The most abundant polyphenols found in WP and PB were p-coumaric and isoferulic acids. Urinary excretion of isoferulic acid increased after the intake of WP and PB (p = 0.032 and p = 0.048, respectively), with no significant difference observed between interventions. Conclusion: The crushing step in peanut butter production seems to enhance the bioavailability of bioactive compounds.
Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs
Authors: González-Arostegui Luis G. , Muñoz-Prieto Alberto , Tvarijonaviciute Asta , Cerón José Joaquín , Rubio Camila Peres
Published: February 2022

The evaluation of the biomarkers of oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole blood (WB), can be used. The objective of this study was to evaluate if a comprehensive panel of redox biomarkers can be measured in the WB and RBCs of dogs, and their possible changes “in vitro” after the addition of different concentrations of ascorbic acid. The panel was integrated by biomarkers of the antioxidant status, such as cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type 1 (PON-1), and of the oxidant status, such as total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). All the assays were precise and accurate in WB and RBCs lysates. In addition, they showed changes after ascorbic acid addition that are in line with previously published results, being WB more sensitive to detect these changes in our experimental conditions. In conclusion, the panel of assays used in this study can be measured in the WB and RBCs of the dog. In particular, the higher sensitivity to detect changes in our experimental conditions and its easier sample preparation makes WB a promising sample for the evaluation of redox status in dogs, with also potential applications to other animal species and humans.
Improvement of Damage in Human Dermal Fibroblasts by 3,5,7-Trimethoxyflavone from Black Ginger (Kaempferia parviflora)
Authors: Lee Sullim , Jang Taesu , Kim Ki Hyun , Kang Ki Sung
Published: February 2022

Reactive oxygen species (ROS) are generated during intrinsic (chronological aging) and extrinsic (photoaging) skin aging. Therefore, antioxidants that inhibit ROS production may be involved in delaying skin aging. In this study, we investigated the potential effects of compounds isolated from black ginger, Kaempferia parviflora, a traditional medicinal plant, on normal human dermal fibroblasts in the context of inflammation and oxidative stress. The isolated compounds were structurally characterized as 5-hydroxy-7-methoxyflavone (1), 3,7-dimethoxy-5-hydroxyflavone (2), 5-hydroxy-3,7,3,4-tetramethoxyflavone (3), 7,4-dimethylapigenin (4), 3,7,4-trimethylkaempferol (5), and 3,5,7-trimethoxyflavone (6), using nuclear magnetic resonance spectroscopy (NMR) and liquid chromatography–mass spectrometry (LC/MS) analyses. These flavonoids were first evaluated for their ability to suppress extracellular matrix degradation in normal human dermal fibroblasts. Of these, 3,5,7-trimethoxyflavone (6) significantly inhibited the tumor necrosis factor (TNF)-α-induced high expression and secretion of matrix metalloproteinase (MMP)-1 by cells. We further found that 3,5,7-trimethoxyflavone suppressed the excessive increase in ROS, mitogen-activated protein kinases (MAPKs), Akt, and cyclooxygenase-2 (COX-2)and increased heme oxygenase (HO)-1 expression. The expression of pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and IL-8, was also suppressed by 3,5,7-trimethoxyflavone (6). Taken together, our results indicate that 3,5,7-trimethoxyflavone (6) isolated from K. parviflora is a potential candidate for ameliorating skin damage.
Prediction of Pile Bearing Capacity Using XGBoost Algorithm: Modeling and Performance Evaluation
Authors: Amjad Maaz , Ahmad Irshad , Ahmad Mahmood , Wróblewski Piotr , Kamiński Paweł , Amjad Uzair
Published: February 2022

The major criteria that control pile foundation design is pile bearing capacity (Pu). The load bearing capacity of piles is affected by the various characteristics of soils and the involvement of multiple parameters related to both soil and foundation. In this study, a new model for predicting bearing capacity is developed using an extreme gradient boosting (XGBoost) algorithm. A total of 200 driven piles static load test-based case histories were used to construct and verify the model. The developed XGBoost model results were compared to a number of commonly used algorithms—Adaptive Boosting (AdaBoost), Random Forest (RF), Decision Tree (DT) and Support Vector Machine (SVM) using various performance measure metrics such as coefficient of determination, mean absolute error, root mean square error, mean absolute relative error, Nash–Sutcliffe model efficiency coefficient and relative strength ratio. Furthermore, sensitivity analysis was performed to determine the effect of input parameters on Pu. The results show that all of the developed models were capable of making accurate predictions however the XGBoost algorithm surpasses others, followed by AdaBoost, RF, DT, and SVM. The sensitivity analysis result shows that the SPT blow count along the pile shaft has the greatest effect on the Pu.
Optimisation of Segregation Distances between Electric Cable Bundles Embedded in a Structure
Authors: Morio Jérôme , Junqua Isabelle , Bertuol Solange , Parmantier Jean-Philippe
Published: February 2022

This paper presents the optimisation of the segregation distance between two electric cable bundles installed in an aircraft structure under electromagnetic compatibility constraints. We first describe the problem formulation where a probabilistic constraint has to be verified during the optimisation process. To overcome the nonlinearity of the constraint function and guarantee the algorithm convergence, we propose a joint approach between Monte Carlo sampling and a Kriging surrogate to estimate the optimum distance with a low computational cost. This methodology was tested on a realistic use-case of distance segregation between cable bundles.
Modeling Random Exit Selection in Intercity Expressway Traffic with Quantum Walk
Authors: Li Dongshuang , Hu Xu , Zhou Xinxin , Luo Wen , Zhu A. Xing , Yu Zhaoyuan
Published: February 2022

In intercity expressway traffic, the multiplicity of available routes leads to randomness in exit selection. Random exit selection by drivers is hard to observe, and thus it is a challenge to model intercity expressway traffic sufficiently. In this paper, we developed a Random Quantum Traffic Model (RQTM), which modeled the stochastic traffic fluctuation caused by random exit selection and the residual regularity fluctuation with the quantum walk and autoregressive moving average model (ARMA), respectively. The RQTM considered the random exit selection of a driver as a quantum stochastic process with a dynamic probability function. A quantum walk was applied to update the probability function, which simulated when and where a driver will leave the expressway. We validated our model with hourly traffic data from seven exits from the Nanjing–Changzhou expressway in eastern China. For the seven exits, the coefficients of determination of the RQTM ranged from 0.5 to 0.85. Compared with the classical random walk and the ARMA model, the coefficients of determination were increased by 21.28% to 104.98%, and the relative mean square error decreased by 11.61% to 32.92%. We conclude that the RQTM provides new potential for modeling traffic dynamics with consideration of unobservable random driver decision making.
The Effect of Marketing Activities on Web Search Volume: An Empirical Analysis of Chinese Film Industry Data
Authors: Yoon Yeujun , Deng Rongchao , Joo Jaewoo
Published: February 2022

Prior research on consumers’ web searches primarily examined the effect of web searches on product sales or the characteristics of the web searchers. Differing from prior research, we investigate the effect of marketing activities on web search volume. We selected 314 movies released in China whose box office revenues were greater than RMB 10,000. Then, we collected data points on web search volume and marketing activities from the Baidu, Sina Weibo, and Douban platforms from the 3 weeks prior to the release of each movie. Marketing activity data points were derived from three sources: news media, social network marketing, and film stars. Our data analysis of 6594 observations revealed two major findings. First, news media, social network marketing, and the effect of film stars increased the web search volumes of the films. In particular, social network marketing had the strongest impact on the web search volume. Second, the previous-day web search volume increased the present-day web search volume without marketing activities, suggesting a spillover effect. We discuss the academic contributions and managerial implications of our findings in the context of online marketing and new product launches.
Influence of Plastic Waste on the Workability and Mechanical Behaviour of Asphalt Concrete
Authors: Fonseca Mariana , Capitão Silvino , Almeida Arminda , Picado-Santos Luís
Published: February 2022

The use of plastic waste as a bitumen extender added throughout the manufacturing process of asphalt concrete contributes value to that type of waste. Moreover, this type of polymer can improve some mechanical properties of asphalt concrete without weakening its workability and other mechanical characteristics too much. The study aimed to address these issues for four types of plastic waste, using different plastic contents added by the dry process and compared the results with a conventional mixture without plastic. A set of laboratory tests, such as volumetric parameter evaluation, the Marshall, gyratory compactor, and indirect tensile tests, repetitive four-point bending; and repetitive compression, assessed the workability and mechanical behavior of the studied materials. The results show that, although the addition of plastic waste reduces workability, the asphalt concrete retains satisfactory handling conditions. By adding plastic waste, the asphalt concrete becomes more elastic, and the stiffness values of the material are adequate to apply the material in a pavement surface layer. The resistance to fatigue cracking was at a suitable level for the asphalt mixtures studied. Adding the plastic waste in the study generally improved resistance to permanent deformation, although the performance was plastic type and content dependent.