bluuebunny/crossref_metadata_2025 huggingface dataset:
Could Chlorella pyrenoidosa be exploited as an alternative nutrition source in aquaculture feed? A study on the nutritional values and anti-nutritional factors
Authors: Chen Fufeng , Qian Jun , He Yu , Leng Yunyue , Zhou Wenguang
Published: December 2022

This work attempted to identify if microalgal biomass can be utilized as an alternative nutrition source in aquaculture feed by analyzing its nutritional value and the anti-nutritional factors (ANFs). The results showed that Chlorella pyrenoidosa contained high-value nutrients, including essential amino acids and unsaturated fatty acids. The protein content in C. pyrenoidosa reached 52.4%, suggesting that microalgal biomass can be a good protein source for aquatic animals. We also discovered that C. pyrenoidosa contained some ANFs, including saponin, phytic acid, and tannins, which may negatively impact fish productivity. The high-molecular-weight proteins in microalgae may not be effectively digested by aquatic animals. Therefore, based on the findings of this study, proper measures should be taken to pretreat microalgal biomass to improve the nutritional value of a microalgae-based fish diet.
Case report: Two unique cases of co-existing primary brain tumors of glial origin in opposite hemispheres
Authors: Singhal Ishan , Coss Dylan , Mueller Wade , Straza Michael , Krucoff Max Ostrinsky , Santos-Pinheiro Fernando
Published: December 2022

Background

Primary CNS tumors are rare. Coexistence of two glial tumors of different histological origins in the same patient is even rarer. Here we describe two unique cases of coexisting distinct glial tumors in opposite hemispheres.

Cases

Patient 1 is a 38-year-old male who presented with a seizure in February/2016. MRI showed a left parietal and a right frontal infiltrating nonenhancing lesions. Both lesions were resected revealing an oligodendroglioma WHO grade-2 and an astrocytoma WHO grade-2. Patient 2 is a 34-year-old male who presented with a seizure in November/2021. MRI showed a left frontal and a right mesial temporal lobe infiltrating nonenhancing lesions. Both lesions were resected revealing an oligodendroglioma WHO grade-2 and a diffuse low-grade glioma, MAPK pathway-altered (BRAF V600E-mutant). Patient 1 underwent adjuvant treatment. Both patients are without recurrence to date.

Discussion

Two histologically distinct glial tumors may coexist, especially when they are non-contiguous. Pathological confirmation of each lesion is imperative for appropriate management. We highlight the different management of gliomas based on the new CNS WHO 2021 classification compared to its 2016 version, based on NCCN guidelines. Although more molecular markers are being incorporated into glioma classification, their clinical impact of it is yet to be determined.
Advanced diagnostic and therapeutic strategies in nanotechnology for lung cancer
Authors: Duan Yujuan , Shen Chen , Zhang Yinan , Luo Yao
Published: December 2022

As a highly invasive thoracic malignancy with increasing prevalence, lung cancer is also the most lethal cancer worldwide due to the failure of effective early detection and the limitations of conventional therapeutic strategies for advanced-stage patients. Over the past few decades, nanotechnology has emerged as an important technique to obtain desired features by modifying and manipulating different objects on a molecular level and gained a lot of attention in many fields of medical applications. Studies have shown that in lung cancer, nanotechnology may be more effective and specific than traditional methods for detecting extracellular cancer biomarkers and cancer cells in vitro, as well as imaging cancer in vivo; Nanoscale drug delivery systems have developed rapidly to overcome various forms of multi-drug resistance and reduce detrimental side effects to normal tissues by targeting cancerous tissue precisely. There is no doubt that nanotechnology has the potential to enhance healthcare systems by simplifying and improving cancer diagnostics and treatment. Throughout this review, we summarize and highlight recent developments in nanotechnology applications for lung cancer in diagnosis and therapy. Moreover, the prospects and challenges in the translation of nanotechnology-based diagnostic and therapeutic methods into clinical applications are also discussed.
The lifestyle factors of physical activity and diet balance associated with HPV infection in China: The cross-sectional study
Authors: Li Yantao , Liu Mengping , Huang Peng , Wang Wenxiang , Jiang Yuxin , Yang Zhongzhou , Wang Anli
Published: December 2022

Background

Human wellbeing has been linked with lifestyle factors such as physical activity, diet balance, sleep quality, depression, and anxiety. However, few studies illustrate the relationship between such lifestyle factors and HPV infection. In this study, we investigated the association between lifestyle factors, age, disease status and HPV infection.

Participants and methods

Participants were recruited through a digital eHealth platform in Shenzhen, Mainland China. Both lifestyle factors and cervicovaginal mucus (CVM) samples to test for HPV outcomes were collected from each participant as a cross-sectional study. In addition, the eHealth platform recorded age and current or history diseases, which were adjusted to apply for both univariable and multivariable logistic regression. Furthermore, lifestyle factors were categorized as different levels to conduct stratification analysis.

Results

We recruited 149 HPV positive and 346 HPV negative participants through HPV detection. Physical activity and diet balance were significantly associated with HPV infection in lifestyle factors (P values < 0.001) after adjusting for age and current or history diseases. However, stratified analysis showed three factors were insignificant for HPV infection – namely, sleep quality, depression, and anxiety. Most HPV infections involved a sole HPV serotype (83%), and diet balance was the most significant difference between sole and multiple HPV infections.

Conclusions

Among lifestyle factors, physical inactivity or diet imbalance can significantly increase HPV infection risk. In particular, diet balance might be related to the number of HPV serotypes. Our results suggest that exercising and regulating diet may reduce the risk of HPV infection.
Preoperative prediction of lymphovascular invasion in patients with T1 breast invasive ductal carcinoma based on radiomics nomogram using grayscale ultrasound
Authors: Xu Mao-Lin , Zeng Shu-E , Li Fang , Cui Xin-Wu , Liu Gui-Feng
Published: December 2022

Purpose

The aim of this study was to develop a radiomics nomogram based on grayscale ultrasound (US) for preoperatively predicting Lymphovascular invasion (LVI) in patients with pathologically confirmed T1 (pT1) breast invasive ductal carcinoma (IDC).

Methods

One hundred and ninety-two patients with pT1 IDC between September 2020 and August 2022 were analyzed retrospectively. Study population was randomly divided in a 7: 3 ratio into a training dataset of 134 patients (37 patients with LVI-positive) and a validation dataset of 58 patients (19 patients with LVI-positive). Clinical information and conventional US (CUS) features (called clinic_CUS features) were recorded and evaluated to predict LVI. In the training dataset, independent predictors of clinic_CUS features were obtained by univariate and multivariate logistic regression analyses and incorporated into a clinic_CUS prediction model. In addition, radiomics features were extracted from the grayscale US images, and the radiomics score (Radscore) was constructed after radiomics feature selection. Subsequent multivariate logistic regression analysis was also performed for Radscore and the independent predictors of clinic_CUS features, and a radiomics nomogram was developed. The performance of the nomogram model was evaluated via its discrimination, calibration, and clinical usefulness.

Results

The US reported axillary lymph node metastasis (LNM) (US_LNM) status and tumor margin were determined as independent risk factors, which were combined for the construction of clinic_CUS prediction model for LVI in pT1 IDC. Moreover, tumor margin, US_LNM status and Radscore were independent predictors, incorporated as the radiomics nomogram model, which achieved a superior discrimination to the clinic_CUS model in the training dataset (AUC: 0.849 vs. 0.747; P < 0.001) and validation dataset (AUC: 0.854 vs. 0.713; P = 0.001). Calibration curve for the radiomic nomogram showed good concordance between predicted and actual probability. Furthermore, decision curve analysis (DCA) confirmed that the radiomics nomogram had higher clinical net benefit than the clinic_CUS model.

Conclusion

The US-based radiomics nomogram, incorporating tumor margin, US_LNM status and Radscore, showed a satisfactory preoperative prediction of LVI in pT1 IDC patients.
Two novel mutations in VPS33B gene cause a milder ARC syndrome with prolonged survival in a 12-year-old patient: Case report
Authors: Zhu Yingjie , Chen Dongmei
Published: December 2022

Arthrogryposis–renal dysfunction–cholestasis (ARC) syndrome is a rare autosomal recessive disease caused by VPS33B and VIPAR gene mutations. The main clinical manifestations are congenital joint contracture, renal dysfunction mainly characterized by distal renal tubular dysfunction, and low glutamyltransferase cholestasis. Most patients with ARC die within 2 years of birth. Here, we report the case of a 12-year-old girl with an ARC phenotype who experienced long-term survival with only mild clinical symptoms. We detected two new heterozygous mutation sites of the VPS33B gene in this child, c.1081C > T (p.GLN361X, 257) and c.244T > C (p.Cys82Arg), through the gene detection technique; the tertiary structure of the protein was predicted by using the SWISS-model. We further reviewed the literature and summarized the clinical manifestations and gene loci of 19 ARC syndrome patients with long-term survival reported so far.
Minimally invasive management of a giant paratubal cyst in an adolescent female: Case report and review of the literature in the pediatric population
Authors: Romeo Paola , Loria Giada , Martinelli Canio , Ercoli Alfredo , Romeo Carmelo
Published: December 2022

Introduction

Paraovarian or paratubal cysts both define cysts located between the ovary and the fallopian tube. They are usually benign and frequently occur in the third and fourth decade of life. Paratubal cysts are defined as giant when they exceed the threshold of 150 mm.

Methods

We report the case of a 15-year-old girl who complained about diffuse abdominal pain since 2 years that was diagnosed with a 196 mm × 90 mm × 267 mm giant paratubal cyst. We furthermore reviewed all the data published on 13 articles, published between 2006 and 2021, concerning giant paraovarian cyst (POC) in pediatric patients.

Results

The giant mass of our 15-year-old patient was removed through a fertility-sparing laparoscopic surgery. Histopathological diagnosis of cystadenofibroma was made up, with no cytologic report of neoplastic cells. The incidence of POC in the pediatric and adolescent population attests around 4%. However, only 12.96% of them are defined giant (larger than 15 cm). Indeed, to the best of our knowledge, only 13 cases of giant paratubal cysts have been reported in adolescents. To accomplish diagnosis and differential diagnosis, accurate history and physical examination are mandatory. In all cases reported in the literature, further instrumental analyses were performed, including ultrasound, CT, and/or MRI scan. International Ovarian Tumor Analysis (IOTA) rules have not yet been validated in the pediatric population. Because of the advantages of the laparoscopic procedures, it is often preferred in pediatric population, also to help preserve as much ovarian parenchyma and salpinx if thought possible. The incidence of malignant adnexal masses in the pediatric population is reported to range from 4% to 9%, accounting for 1% of all pediatric cancers.

Conclusion

Giant paratubal cysts in adolescent females are extremely rare and usually benign. A fertility-sparing laparoscopic surgery should be the preferable option whenever possible. Considering the rarity of these conditions, further investigations are needed to exclude the possibility of a malignant evolution.
Intravitreal vascular endothelial growth factor inhibitors did not increase the risk of end-stage renal disease in patients with biopsy-proven diabetic kidney disease based on matched study
Authors: Xiao Xiang , Zhang Junlin , Ji Shuming , Zou Yutong , Wu Yucheng , Qin Chunmei , Yang Jia , Zhao Yuancheng , Yang Qin , Liu Fang
Published: December 2022

Purpose: This study aimed to investigate the effects of intravitreal (IVT) VEGFi on long-term renal outcomes in patients with biopsy-proven diabetic kidney disease (DKD).

Patients and methods: Patients prescribed IVT VEGFi (VEGFi group) were enrolled from a retrospective cohort with biopsy-proven DKD, and those not prescribed VEGFi (non-VEGFi group) were enrolled by 1:3 propensity score matching, adjusted for clinical and pathological baseline indicators. The primary endpoint is defined as end-stage renal disease (ESRD) and the secondary endpoint is defined as all-cause mortality.

Results: Compared with patients in non-VEGFi group, patients with VEGFi had a higher proportion of diabetic retinopathy (DR) (50.9% vs 100%, p < 0.001) before matching. Standardized mean difference (SMD) of age, DR, duration of diabetes, the proportion of hypertension, eGFR, initial proteinuria, serum albumin, hemoglobin, the proportion of RAAS inhibitor and interstitial fibrosis and tubular atrophy (IFTA) were >10%. After matching, there was no significant difference in clinical pathology between the two groups. Except for the proportion of hypertension, the SMD of other indicators was <10%. Endpoints such as ESRD (Log-Rank p = 0.772) and all-cause mortality (Log-Rank p = 0.834) were not significantly different between the two groups.

Conclusion: Our data suggested that IVT VEGFi did not increase the incidence of ESRD and all-cause mortality in patients with DKD.
3CLpro inhibitors: DEL-based molecular generation
Authors: Xiong Feng , Xu Honggui , Yu Mingao , Chen Xingyu , Zhong Zhenmin , Guo Yuhan , Chen Meihong , Ou Huanfang , Wu Jiaqi , Xie Anhua , Xiong Jiaqi , Xu Linlin , Zhang Lanmei , Zhong Qijian , Huang Liye , Li Zhenwei , Zhang Tianyuan , Jin Feng , He Xun
Published: December 2022

Molecular generation (MG) via machine learning (ML) has speeded drug structural optimization, especially for targets with a large amount of reported bioactivity data. However, molecular generation for structural optimization is often powerless for new targets. DNA-encoded library (DEL) can generate systematic, target-specific activity data, including novel targets with few or unknown activity data. Therefore, this study aims to overcome the limitation of molecular generation in the structural optimization for the new target. Firstly, we generated molecules using the structure-affinity data (2.96 million samples) for 3C-like protease (3CLpro) from our own-built DEL platform to get rid of using public databases (e.g., CHEMBL and ZINC). Subsequently, to analyze the effect of transfer learning on the positive rate of the molecule generation model, molecular docking and affinity model based on DEL data were applied to explore the enhanced impact of transfer learning on molecule generation. In addition, the generated molecules are subjected to multiple filtering, including physicochemical properties, drug-like properties, and pharmacophore evaluation, molecular docking to determine the molecules for further study and verified by molecular dynamics simulation.
Creatine modulates cellular energy metabolism and protects against cancer cachexia-associated muscle wasting
Authors: Wei Lulu , Wang Ranran , Lin Kai , Jin Xiaolu , Li Li , Wazir Junaid , Pu Wenyuan , Lian Panpan , Lu Renwei , Song Shiyu , Zhao Quan , Li Jiabin , Wang Hongwei
Published: December 2022

Cancer cachexia is a multifactorial syndrome defined by progressive loss of body weight with specific depletion of skeletal muscle and adipose tissue. Since there are no FDA-approved drugs that are available, nutritional intervention is recommended as a supporting therapy. Creatine supplementation has an ergogenic effect in various types of sports training, but the regulatory effects of creatine supplementation in cancer cachexia remain unknown. In this study, we investigated the impact of creatine supplementation on cachectic weight loss and muscle loss protection in a tumor-bearing cachectic mouse model, and the underlying molecular mechanism of body weight protection was further assessed. We observed decreased serum creatine levels in patients with cancer cachexia, and the creatine content in skeletal muscle was also significantly decreased in cachectic skeletal muscle in the C26 tumor-bearing mouse model. Creatine supplementation protected against cancer cachexia-associated body weight loss and muscle wasting and induced greater improvements in grip strength. Mechanistically, creatine treatment altered the dysfunction and morphological abnormalities of mitochondria, thus protecting against cachectic muscle wasting by inhibiting the abnormal overactivation of the ubiquitin proteasome system (UPS) and autophagic lysosomal system (ALS). In addition, electron microscopy revealed that creatine supplementation alleviated the observed increase in the percentage of damaged mitochondria in C26 mice, indicating that nutritional intervention with creatine supplementation effectively counteracts mitochondrial dysfunction to mitigate muscle loss in cancer cachexia. These results uncover a previously uncharacterized role for creatine in cachectic muscle wasting by modulating cellular energy metabolism to reduce the level of muscle cell atrophy.
First-line sintilimab plus chemotherapy in locally advanced or metastatic esophageal squamous cell carcinoma: A cost-effectiveness analysis from China
Authors: Shen Jian , Du Yi , Shao Rong , Jiang Rong
Published: December 2022

Objective: The study aimed to assess the cost-effectiveness of sintilimab combined with cisplatin plus paclitaxel versus chemotherapy alone as first-line treatment in patients with advanced or metastatic esophageal squamous cell carcinoma from the Chinese healthcare system.

Materials and methods: A partitioned survival model was developed based on the ORIENT-15 clinical trial. Drug costs and health state utility were obtained from the literature. Outcomes included the health outcomes in life-years, quality-adjusted life-years (QALYs), and the incremental cost-effectiveness ratio. One-way and probabilistic sensitivity analyses were performed to evaluate the model uncertainty.

Result: In overall population, patients given sintilimab plus chemotherapy gained more health benefits (0.90 QALYs vs. 0.61 QALYs), and the cost was more (15,399.21 US$ VS. 7475.58 US$) than that for patients in the chemotherapy group. In the subgroup, patients given sintilimab plus chemotherapy gained more health benefits (0.89 QALYs vs. 0.68 QALYs), and the cost was more (15,656.19 US$ vs. 9,162.77 US$) than that for patients in the chemotherapy group. Compared with chemotherapy, patients receiving sintilimab plus chemotherapy had ICERs of $26,773.68/QALY in the overall population and $30,065.50/QALY in the subgroup, which was above the threshold of WTP.

Conclusion: Sintilimab plus chemotherapy was more cost-effective than chemotherapy alone for patients with advanced esophageal cancer from the perspective of the Chinese healthcare system.
Interdependence design principles in practice
Authors: Vignati Micael , Johnson Matthew , Bunch Larry , Carff John , Duran Daniel
Published: December 2022

Adaptability lies at the heart of effective teams and it is through management of interdependence that teams are able to adapt. This makes interdependence a critical factor of human-machine teams. Nevertheless, engineers building human-machine systems still rely on the same tools and techniques used to build individual behaviors which were never designed to address the complexity that stems from interdependence in joint activity. Many engineering approaches lack any systematic rigor and formal method for identifying, managing and exploiting interdependence, which forces ad hoc solutions or workarounds. This gap between theories of interdependence and operable tooling leaves designers blind to the issues and consequences of failing to adequately address interdependence within human-machine teams. In this article, we propose an approach to operationalizing core concepts needed to address interdependence in support of adaptive teamwork. We describe a formalized structure, joint activity graphs, built on interdependence design principles to capture the essence of joint activity. We describe the runtime requirements needed to dynamically exploit joint activity graphs and to support intelligent coordination during execution. We demonstrate the effectiveness of such a structure at supporting adaptability using the Capture-the-Flag domain with heterogeneous teams of unmanned aerial vehicles and unmanned ground systems. In this dynamic adversarial domain, we show how agents can make use of the information provided by joint activity graphs to generally and pragmatically react and adapt to perturbations in the joint activity, the environment, or the team and explicitly manage and exploit interdependence to produce effective teamwork. In doing so, we demonstrate how flexible and adaptive teamwork can be achieved through formally guided design that supports effective management of interdependence.
Internal translation of Gja1 (Connexin43) to produce GJA1-20k: Implications for arrhythmia and ischemic-preconditioning
Authors: Whisenant Claire C. , Shaw Robin M.
Published: December 2022

Internal translation is a form of post-translation modification as it produces different proteins from one mRNA molecule by beginning translation at a methionine coding triplet downstream of the first methionine. Internal translation can eliminate domains of proteins that otherwise restrict movement or activity, thereby creating profound functional diversity. Connexin43 (Cx43), encoded by the gene Gja1, is the main gap junction protein necessary for propagating action potentials between adjacent cardiomyocytes. Gja1 can be internally translated to produce a peptide 20 kD in length named GJA1-20k. This review focuses on the role of GJA1-20k in maintaining cardiac electrical rhythm as well as in ischemic preconditioning (IPC). Connexin43 is the only ion channel we are aware that has been reported to be subject to internal translation. We expect many other ion channels also undergo internal translation. The exploration of post-translational modification of ion channels, and in particular of internal translation, has the potential to greatly increase our understanding of both canonical and non-canonical ion channel biology.
Data-centric annotation analysis for plant disease detection: Strategy, consistency, and performance
Authors: Dong Jiuqing , Lee Jaehwan , Fuentes Alvaro , Xu Mingle , Yoon Sook , Lee Mun Haeng , Park Dong Sun
Published: December 2022

Object detection models have become the current tool of choice for plant disease detection in precision agriculture. Most existing research improved the performance by ameliorating networks and optimizing the loss function. However, because of the vast influence of data annotation quality and the cost of annotation, the data-centric part of a project also needs more investigation. We should further consider the relationship between data annotation strategies, annotation quality, and the model’s performance. In this paper, a systematic strategy with four annotation strategies for plant disease detection is proposed: local, semi-global, global, and symptom-adaptive annotation. Labels with different annotation strategies will result in distinct models’ performance, and their contrasts are remarkable. An interpretability study of the annotation strategy is conducted by using class activation maps. In addition, we define five types of inconsistencies in the annotation process and investigate the severity of the impact of inconsistent labels on model’s performance. Finally, we discuss the problem of label inconsistency during data augmentation. Overall, this data-centric quantitative analysis helps us to understand the significance of annotation strategies, which provides practitioners a way to obtain higher performance and reduce annotation costs on plant disease detection. Our work encourages researchers to pay more attention to annotation consistency and the essential issues of annotation strategy. The code will be released at: https://github.com/JiuqingDong/PlantDiseaseDetection_Yolov5 .
Opposite roles of MAPKKK17 and MAPKKK21 against Tetranychus urticae in Arabidopsis
Authors: Romero-Hernandez Gara , Martinez Manuel
Published: December 2022

After recognizing a biotic stress, plants activate signalling pathways to fight against the attack. Typically, these signalling pathways involve the activation of phosphorylation cascades mediated by Mitogen-Activated Protein Kinases (MAPKs). In the Arabidopsis thaliana-Tetranychus urticae plant-herbivore model, several Arabidopsis MAP kinases are induced by the mite attack. In this study, we demonstrate the participation of the MEKK-like kinases MAPKKK17 and MAPKKK21. Leaf damage caused by the mite was assessed using T-DNA insertion lines. Differential levels of damage were found when the expression of MAPKKK17 was increased or reduced. In contrast, reduced expression of MAPKKK21 resulted in less damage caused by the mite. Whereas the expression of several genes associated with hormonal responses did not suffer significant variations in the T-DNA insertion lines, the expression of one of these kinases depends on the expression of the other one. In addition, MAPKKK17 and MAPKKK21 are coexpressed with different sets of genes and encode proteins with low similarity in the C-terminal region. Overall, our results demonstrate that MAPKKK17 and MAPKKK21 have opposite roles. MAPKKK17 and MAPKKK21 act as positive and negative regulators, respectively, on the plant response. The induction of MAPKKK17 and MAPKKK21 after mite infestation would be integrated into the bulk of signalling pathways activated to balance the response of the plant to a biotic stress.
An exotic plant successfully invaded as a passenger driven by light availability
Authors: Liu Yanyan , Li Wenjun , Sui Xiaolin , Li Airong , Li Kaihui , Gong Yanming
Published: December 2022

Invasive exotic plant species (IEPs) are widely distributed across the globe, but whether IEPs are drivers or passengers of habitat change in the invaded spaces remains unclear. Here, we carried out a vegetation and soil survey in 2018 and two independent field experiments (Pedicularis kansuensisremoval in 2014 and 2015, and fertilization experiment since 2012) and found that the invasive annualP. kansuensiswas at a disadvantage in light competition compared with perennial native grasses, but the successful invasion ofP. kansuensiswas due to the sufficient light resources provided by the reduced coverage of the native species. Conversely, nitrogen enrichment can effectively inhibitP. kansuensisinvasion by increasing the photocompetitive advantage of the native species. sP. kansuensisinvasion did not reduce species richness, but did increase plant community coverage, productivity and soil nutrients. Furthermore, the removal ofP. kansuensishad little effect on the plant community structure and soil properties. Our results suggest that the passenger model perfectly explains the benign invasive mechanism ofP. kansuensis. The invasion “ticket” ofP. kansuensisis a spare ecological niche for light resources released by overgrazing.
Survey and detection for citrus tristeza virus in Florida groves with an unconventional tool: The Asian citrus psyllid
Authors: Britt-Ugartemendia Kellee , Turner Donielle , Sieburth Peggy , Batuman Ozgur , Levy Amit
Published: December 2022

The citrus industry of Florida faces insurmountable challenges against the destructive diseases citrus tristeza and Huanglongbing (HLB, or citrus greening). Though the tristeza causal agent, citrus tristeza virus (CTV), has been in Florida decades longer than HLB, growers have concentrated most of their efforts on combating the more detrimental HLB. The Asian citrus psyllid (Diaphorina citri; ACP) is the insect vector of the bacterial pathogen Candidatus Liberibacter asiaticus and transmits the incurable HLB to all commercial citrus. During our searches for biological and viral controls against the ACP, we consistently detected sequences of CTV in Florida field populations of ACP. This unexpected finding led us to investigate whether ACPs collected from young shoots could be used as a tool to survey CTV in Florida citrus groves. We first surveyed for the most common CTV strains in Florida (T30, T36, and VT/T68) in citrus trees on mostly sour orange (Citrus aurantium) rootstock, the rootstock susceptible to CTV decline. Out of 968 trees sampled across five years (2018-2022), approximately 8.2% were positive for CTV, with more than half of the CTV-positive trees infected with strain T30. Simultaneously, we looked at CTV strains in ACPs during this time and found that approximately 88% of pooled adult and nymph ACPs also had CTV, with over half the positive samples having the T36 strain. As a result of the much higher CTV incidences in the ACPs, we conducted a second investigation into whether we could more easily detect the same CTV strains in ACP nymphs as in CTV-infected citrus tissue. After individually sampling 43 trees and pooling the nymphs from each tree, we detected CTV at about the same incidence in the citrus tissue and the nymphs, but with much less ACP tissue, time, and resources required for detection compared to citrus tissue. Results from this study illustrate the sustained threat of CTV to Florida citrus and demonstrate the ACP as a potential bioindicator for CTV.
Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation
Authors: Liang Xiao , Chen Qing , Liu Ying , Wu Chunling , Li Kaimian , Wu Mufeng , Yao Xiaowen , Qiao Yang , Zhang Yao , Geng Yue
Published: December 2022

Introduction

Utilization of resistant germplasm is considered as an effective, economical and eco-friendly strategy for cassava pest management. Tetranychus urticae, known as the two-spotted spider mite (TSSM), is a devastating pest in Asian cassava planting countries as well as in China. However, the resistant levels of abundant cassava germplasms to TSSM remains largely unknown.

Methods

To fill this knowledge gap, we conducted screening of 202 cassava germplasm for resistance to TSSM in China based on the classification of mite damage phenotype, under both greenhouse and field conditions.

Results

The three rounds of large-scale greenhouse experiments had identified two highly resistant (HR) varieties (C1115 and MIANDIAN), five resistant (R) varieties (SC5, SC9, SC15, COLUMBIA-4D and LIMIN) and five highly susceptible (HS) varieties (KU50, BREAD, SC205, TMS60444 and BRA900), besides, these ‘HR’ and ‘R’ varieties would significantly repress the normal development and reproduction of TSSM. In addition, the 12 cassava varieties selected from the greenhouse screening were further subjected to consecutive five years of field validation at Danzhou, Wuming and Baoshan. The seven resistant varieties not only exhibited stable TSSM-resistance performance across the three field environments, but also possessed the same resistant levels as the greenhouse identification, while the resistant varieties SC5 was an exception, which was identified as moderate resistant in Baoshan, indicating the variety-environment interaction may affect its resistance. Furthermore, regional yield estimation suggested that the higher the resistance level was, the better capacity in reducing the yield losses.

Discussion

This study demonstrated that the TSSM-resistant varieties could be considered as ideal materials in mite control or in future breeding programme of mite-resistant cassava plant.
Subgenome-biased expression and functional diversification of a Na+/H+ antiporter homoeologs in salt tolerance of polyploid wheat
Authors: Zheng Mei , Li Jinpeng , Zeng Chaowu , Liu Xingbei , Chu Wei , Lin Jingchen , Wang Fengzhi , Wang Weiwei , Guo Weilong , Xin Mingming , Yao Yingyin , Peng Huiru , Ni Zhongfu , Sun Qixin , Hu Zhaorong
Published: December 2022

Common wheat (Triticum aestivum, BBAADD) is an allohexaploid species combines the D genome from Ae. tauschii and with the AB genomes from tetraploid wheat (Triticum turgidum). Compared with tetraploid wheat, hexaploid wheat has wide-ranging adaptability to environmental adversity such as salt stress. However, little is known about the molecular basis underlying this trait. The plasma membrane Na+/H+ transporter Salt Overly Sensitive 1 (SOS1) is a key determinant of salt tolerance in plants. Here we show that the upregulation of TaSOS1 expression is positively correlated with salt tolerance variation in polyploid wheat. Furthermore, both transcriptional analysis and GUS staining on transgenic plants indicated TaSOS1-A and TaSOS1-B exhibited higher basal expression in roots and leaves in normal conditions and further up-regulated under salt stress; while TaSOS1-D showed markedly lower expression in roots and leaves under normal conditions, but significant up-regulated in roots but not leaves under salt stress. Moreover, transgenic studies in Arabidopsis demonstrate that three TaSOS1 homoeologs display different contribution to salt tolerance and TaSOS1-D plays the prominent role in salt stress. Our findings provide insights into the subgenomic homoeologs variation potential to broad adaptability of natural polyploidy wheat, which might effective for genetic improvement of salinity tolerance in wheat and other crops.
Introducing an adolescent cognitive maturity index
Authors: El Damaty Shady , Darcey Valerie L. , McQuaid Goldie A. , Picci Giorgia , Stoianova Maria , Mucciarone Veronica , Chun Yewon , Laws Marissa L. , Campano Victor , Van Hecke Kinney , Ryan Mary , Rose Emma Jane , Fishbein Diana H. , VanMeter Ashley S.
Published: December 2022

Children show substantial variation in the rate of physical, cognitive, and social maturation as they traverse adolescence and enter adulthood. Differences in developmental paths are thought to underlie individual differences in later life outcomes, however, there remains a lack of consensus on the normative trajectory of cognitive maturation in adolescence. To address this problem, we derive a Cognitive Maturity Index (CMI), to estimate the difference between chronological and cognitive age predicted with latent factor estimates of inhibitory control, risky decision-making and emotional processing measured with standard neuropsychological instruments. One hundred and forty-one children from the Adolescent Development Study (ADS) were followed longitudinally across three time points from ages 11–14, 13–16, and 14–18. Age prediction with latent factor estimates of cognitive skills approximated age within ±10 months (r = 0.71). Males in advanced puberty displayed lower cognitive maturity relative to peers of the same age; manifesting as weaker inhibitory control, greater risk-taking, desensitization to negative affect, and poor recognition of positive affect.
Effects of filial piety belief on cyberbullying perpetration of Chinese university students
Authors: Wei Hua , Lu Lijun , Liu Meiting
Published: December 2022

Cyberbullying has become a worldwide phenomenon. Although the topic has drawn decent academic attention and many studies have been conducted on Chinese samples, variable interests in these studies have not captured the thinking and behavioral characteristics of Chinese people. Based on the dual filial piety model and self-determination theory, this study examined the effect of filial piety belief on cyberbullying perpetration and tested the mediation of relatedness need satisfaction. A total of 856 university students completed the questionnaires, including dual filial piety scale, relatedness need satisfaction scale and cyberbullying perpetration scale. The regression results found that reciprocal filial piety negatively predicted and authoritarian filial piety positively predicted cyberbullying perpetration. The SEM results showed that reciprocal filial piety and authoritarian filial piety impacted cyberbullying perpetration through the mediating effect of relatedness need satisfaction. Reciprocal filial piety was positively while authoritarian filial piety negatively correlated with relatedness need satisfaction and relatedness need satisfaction was negatively correlated with cyberbullying perpetration. The results provide a new position to understand the effect of family factors on cyberbullying perpetration by placing the topic within traditional Chinese family value.
Does believing in different types of religion affect subjective wellbeing? Analysis of the public data of the Taiwan Social Change Survey
Authors: Ding Yu , Huo Weidong , Jin Yaning
Published: December 2022

Previous studies have revealed the impact of objective material conditions and psychological factors, such as the influence of religion on subjective well-being, but have disregarded the role played by differences of religious types formed in Asian cultural and historical contexts. Against this background, the present study aims to examine the association between religious type and subjective wellbeing and its mechanisms – the mediating role of frequency of religious activity and the moderating role of urban-rural areas. This study used Taiwan Social Change Survey (TSCS) 2018 data for researching. The results show that the discrepancy in the frequency of participation in religious activity caused by different types of religious belief will influence personal wellbeing. Respondents who adhere to institutional religion have a higher frequency of participating in religious activities, which has a positive impact on subjective wellbeing. Moreover, further examination shows that urban–rural areas play an important moderating role: respondents living in urban areas are more inclined to participate in religious activities frequently to gain a sense of wellbeing.
How data knowledge transformation affects teachers' precision teaching ability: The mediating effect of data consciousness
Authors: Tao Jia , Fan Chenchen , Wu Wenwen , Zhu Yongyuan
Published: December 2022

With the development of smart education, teachers' precision teaching ability has increasingly become an important component of their professional ability. Based on the Socialization, Externalization, Combination, Internalization (SECI) model of knowledge management, this research constructs a theoretical model of data knowledge transformation, data consciousness, and teachers' precision teaching ability, and uses Partial Least Squares to analyze the interaction and influence among the elements. The empirical results show that the transformation of internal knowledge related to data has a significant positive impact on the improvement of teachers' precision teaching ability and data consciousness; the transformation of external knowledge related to teaching has a positive effect on the teaching management ability of teachers' precision teaching ability, but the effect is not significant. Data consciousness plays a partial mediating effect between internal knowledge transformation and teachers' precision teaching ability, and a complete mediating effect between external knowledge transformation and teaching management ability.
Reinventing the public square and early educational settings through culturally informed, community co-design: Playful Learning Landscapes
Authors: Pesch Annelise , Ochoa Karlena D. , Fletcher Katelyn K. , Bermudez Vanessa N. , Todaro Rachael D. , Salazar Julie , Gibbs Hailey M. , Ahn June , Bustamante Andres S. , Hirsh-Pasek Kathy
Published: December 2022

What if the environment could be transformed in culturally-responsive and inclusive ways to foster high-quality interactions and spark conversations that drive learning? In this article, we describe a new initiative accomplishing this, called Playful Learning Landscapes (PLL). PLL is an evidence-based initiative that blends findings from the science of learning with community-based participatory research to transform physical public spaces and educational settings into playful learning hubs. Here, we describe our model for conducting this research, which is mindful of three key components: community input, how children learn best, and what children need to learn to be successful in the 21st century economy. We describe how this model was implemented in two PLL case studies: one in a predominantly Latine community and the second in early childhood education classrooms. Furthermore, we describe how research employing our model can be rigorously and reliably evaluated using observational and methodological tools that respond to diverse cultural settings and learning outcomes. For example, our work evaluates how PLL impacts adult–child interaction quality and language use, attitudes about play and learning, and community civic engagement. Taken together, this article highlights new ways to involve community voices in developmental and educational research and provides a model of how science can be translated into practice and evaluated in culturally responsive ways. This synthesis of our process and evaluation can be used by researchers, policymakers, and educators to reimagine early educational experiences with an eye toward the built environment that children inhabit in everyday life, creating opportunities that foster lifelong learning.
Academic motivation at early ages: Spanish validation of the Elementary School Motivation Scale (ESMS-E)
Authors: Ramos Marta , De Sixte Raquel , Jáñez Álvaro , Rosales Javier
Published: December 2022

The Elementary School Motivation Scale (ESMS) is the only validated and adapted instrument to assess motivation in early ages and in specific domains using the Self-Determination Theory. The present study aims to validate the ESMS in Spanish population (ESMS-E). To this end, 1,190 students from the first half of Elementary education (6–10 years old) filled in the ESMS-E. To translate the instrument the back translation method was used. Internal consistency was assessed through composite reliability (CR), correlations among the dimensions and Confirmatory Factor Analysis (CFA) were used to analyze the theoretical structure proposed by the original instrument. Results showed optimal internal consistency in the different dimensions (CR = 0.701–0.901) and showed a great fit for the model (RMSEA = 0.064, CFI = 0.929, TLI = 0.913), confirming the original model with nine dimensions and the self-determination continuum. The ESMS-E is valid and reliable in the Spanish version. This validation offers a tool for researchers interested in exploring the motives that drive students in early stages in relation to specific learning domains (i.e., reading, writing and mathematics).
Growth and Hope after loss: How TAPS facilitates posttraumatic growth in those grieving military deaths
Authors: Moore Melinda , Palmer Jerry K. , Cerel Julie , Ruocco Kim
Published: December 2022

We examined posttraumatic growth for 691 participants of the Tragedy Assistance Program for Survivors (TAPS). Peer mentors of bereaved individuals experienced greater posttraumatic growth (PTG) and reported higher psychological health than those who were non-peer mentors. Active involvement in TAPS and resilience consistently and positively predicted all types of PTG. These prediction models were far stronger (R2, AIC) for the suicide-bereaved sample than those bereaved by other causes, and post-hoc analyses suggest suicide-bereaved benefitted more than those bereaved by other causes from active participation in TAPS.
Hand hygiene compliance and associated factor among nurses working in public hospitals of Hararghe zones, Oromia region, eastern Ethiopia
Authors: Umar Hamza , Geremew Abraham , Worku Kassie Teshager , Dirirsa Gebisa , Bayu Kefelegn , Mengistu Dechasa Adare , Berhanu Ashenafi , Mulat Salie
Published: December 2022

Background

Healthcare workers are in constant contact with a wide variety of materials and surfaces, including waste, body fluids, mucous membranes, food, their own bodies, and the skin of patients. As a result, their hands are colonized by different groups of pathogens. Hand hygiene of healthcare workers is recognized to be the main factor in reducing healthcare-associated infections. Therefore, this study aimed to assess hand hygiene adherence and related factors among nurses working in public hospitals in eastern Ethiopia.

Methods

An institutional based cross-sectional study was conducted in Hospital, Hararghe zone, Eastern Ethiopia from July 1 to 30, 2021. A total of 451 study participants were randomly selected, after the proportional allocation of study participants to each selected hospital. The data was collected using self-administered questionnaire and observation checklist. SPSS version 26 was used to analyze the data. Bivariable and multivariable analysis were employed to assess the association between outcome and independent variables. Finally, a p-value of < 0.05 was used as a cutoff point for statistical significance.

Results

Out of 436 eligible nurses, the overall hand hygiene compliance was 37.4% [95% CI (0.33, 0.42)]. The overall compliance among those working in medical, surgical, OR ward, OPD, Gynecology/obstetrics, emergency ward, Intensive care units, Pediatrics, and other wards/departments was 46.8, 44.8, 35.7, 28.2, 20.7, 45.1, 23.1, 40.5, and 29.4%, respectively. The mean knowledge score was 21.6% (SD: 2.08). Furthermore, there was a statistically significant association between hand hygiene compliance and gender, work experience, training in hand hygiene, availability of running water, and knowledge of hand hygiene.

Conclusion

The current study found overall compliance with hand hygiene accounted for 34.7%. Therefore, an exemplary worker may initiate others to do so, and strong managerial and leadership commitment may also help the workers stick to the rules and regulations to follow the multimodal hand hygiene practice as per WHO recommendation.
How do digital lives affect resident mental health in the digital era? Empirical evidence based on Chinese general social survey
Authors: Chen Yan , Wei Mengyang , Ortiz Jaime
Published: December 2022

Having good mental health means we are better able to connect, function, cope and thrive. The widespread application of digital technology in daily life provides new ways and promising tools for residents to maintain their mental health. Given the importance of mental health for everyone, and the fact that mental health problems are prevalent worldwide, this study discusses how digital lives affects the mental health of residents. The results suggest that digital lives are significantly and positively associated with mental health. Mechanisms analysis identifies personal perceptions (self-rated physical exercise and subjective wellbeing) as the important paths for digital lives to promote mental health, while social perceptions (social trust and social fairness) play a suppressing effect on the relationship between them. The results of further discussion show that the degree of the influence of digital lives on mental health of individuals is heterogeneous among different regions. Due to the difference in development level, the positive impact of digital lives is greater in urban areas than in rural areas, and it is stronger in western regions than in eastern and central regions. This study enriches the nascent research stream of digitalization, explores new paths of harnessing digital technologies for mental health, and offers useful insights for the government to guide them in formulating digital development strategies and achieving the Healthy China Strategy.