bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis
Authors: Kanokudom Sitthichai , Assawakongkarat Thachaporn , Akeda Yukihiro , Ratthawongjirakul Panan , Chuanchuen Rungtip , Chaichanawongsaroj Nuntaree
Published: March 2021

The emergence and dissemination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is a global health issue. Food-producing animals, including pigs, are significant reservoirs of antimicrobial resistance (AMR), which can be transmitted to humans. Thus, the rapid detection of ESBLs is required for efficient epidemiological control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was established for the rapid and simultaneous detection of multiple bla genes in a single reaction. Visible blue lines, indicating the presence of the blaCTX-M, blaSHV, and blaOXA genes, were observed within 10 min by the naked eye. The limit of detection of all three genes was 2.5 ng/25 μL, and no cross-reactivity with seven commensal aerobic bacteria was observed. A total of 93.9% (92/98) and 96% (48/50) of the E. coli isolates from pork meat and fecal samples, respectively, expressed an ESBL-producing phenotype. Nucleotide sequencing of the PCR amplicons showed that blaCTX-M was the most prevalent type (91.3–95.83%), of which the main form was blaCTX-M-55. The sensitivity and specificity of the RPA-LFA were 99.2% and 100%, respectively, and were in almost perfect agreement (κ = 0.949–1.000) with the results from PCR sequencing. Thus, the RPA-LFA is a promising tool for rapid and equipment-free ESBL detection and may facilitate clinical diagnosis in human and veterinary medicine, as well as AMR monitoring and surveillance.
Text message reminders for adolescents with poorly controlled type 1 diabetes: A randomized controlled trial
Authors: Ibrahim Nour , Treluyer Jean-Marc , Briand Nelly , Godot Cécile , Polak Michel , Beltrand Jacques
Published: March 2021

Background

Among adolescents with type 1 diabetes, some experience great difficulties with treatment adherence, putting them at high risk of complications. We assessed the effect of text messaging (Short Messaging Service [SMS]) on glycemic control.

Methods

A two-arm open label randomized controlled trial enrolled adolescents with type 1 diabetes aged 12–21 years with baseline HbA1c ≥ 69 mmol/mol (8.5%). The intervention group received daily SMS reminders at self-selected times about insulin injections while the control group received standard of care. The patients allocated to the control group were not aware of the intervention.

Results

92 patients were randomized, 45 in the SMS arm and 47 in the control arm. After 6 months, median HbA1c level was significantly lower in the intervention arm: 73 mmol/mol (8.8%) in the SMS arm and 83 mmol/mol (9.7%) in the control arm in the intent-to-treat analysis (P= 0.03) but no longer in the per protocol analysis (P = 0.65). When we consider the proportions of patients whose HbA1c level decreased by at least 1% between baseline and 6 months, we find a significant difference among patients whose baseline HbA1c was ≥ 80 mmol/mol (9.5%) (n = 56): 60% in the SMS arm and 30.6% in the control arm had lowered their HbA1c level (P= 0.03) in the intent-to-treat analysis but not in the per-protocol analysis (P = 0.50). Patients in the SMS arm reported high satisfaction with the intervention.

Conclusions

While there is a trend to lower HbA1c in the intervention group, no firm conclusions can yet be drawn. Further studies are needed to address methodological issues as we believe these interventions can support behavior change among adolescents with poorly controlled type 1 diabetes. ClinicalTrials.gov identifier:NCT02230137.
Peer support workers in co-production and co-creation in public mental health and addiction services: Protocol for a scoping review
Authors: Aakerblom Kristina Bakke , Ness Ottar
Published: March 2021

Worldwide, there is a growing interest to employ people with lived experiences in health and social services. Particularly in mental health and addiction services, individuals with lived experience of mental health problems enter the workplace as peer support workers (PSW´s). Their aim in the services is to bring in the perspective of service users in interactive processes at the micro and macro levels. The services´ ability to exploit the knowledge from PSW´s lived experiences will influence both the content and quality of the services, its effectiveness and its capacity to innovate and change. The concepts of co-production and co-creation are used to describe these interactive processes in the services in the literature. While co-production is aimed at improving individual services, co-creation seeks to develop service systems. This scoping review aims to provide an overview of the research status of PSW´s different involvement, in co-production and co-creation, in public mental health and addiction services. Studies describing PSW´s involvement in co-production and co-creation will be contrasted and compared. Knowledge about PSW´s involvement in co-production and co-creation is vital for understanding and further developing these interactive processes with PSW´s. The studies reviewed will describe PSW´s different types of involvement in co-production and co-creation in public mental health and addiction services or across organizational and institutional boundaries. The research question is: How are peer support workers involved in co-production and co-creation in public mental health and addiction services, and what are the described outcomes? Literature searches are conducted in Medline, PsycINFO, Embase, Oria, WorldCat, Google Scholar, Scopus, Academic Search Elite, Cinahl, and Web of Science, from the inception of each database to January 4, 2021. Expected results are that PSW´s are often described as a frontline worker who spends most of their working hours in a joint effort to co-produce with service users. Fewer studies describe PSW´s involved in interactive processes to re-design or transform public services systems. It is anticipated that this scoping review will increase the knowledge of the services’ abilities to exploit PSW´s expertise and inform policy and research.
Correlation of student performance on clerkship with quality of medical chart documentation in a simulation setting
Authors: Komasawa Nobuyasu , Terasaki Fumio , Nakano Takashi , Kawata Ryo
Published: March 2021

Background

Medical chart documentation is an essential skill acquired in a clinical clerkship (CC). However, the utility of medical chart writing simulations as a component of the objective structured clinical examination (OSCE) has not been sufficiently evaluated. In this study, medical chart documentation in several clinical simulation settings was performed as part of the OSCE, and its correlation with CC performance was evaluated.

Methods

We created a clinical situation video and images involving the acquisition of informed consent, cardiopulmonary resuscitation, and diagnostic imaging in the emergency department, and assessed medical chart documentation performance by medical students as part of the OSCE. Evaluations were conducted utilizing original checklist (0–10 point). We also analyzed the correlation between medical chart documentation OSCE scores and CC performance of 120 medical students who performed their CC in 2019 as 5th year students and took the Post-CC OSCE in 2020 as 6th year students.

Results

Of the OSCE components, scores for the acquisition of informed consent and resuscitation showed significant correlations with CC performance (P<0.001 for each). In contrast, scores for diagnostic imaging showed a slightly positive, but non-significant, correlation with CC performance (P = 0.107). Overall scores for OSCE showed a significant correlation with CC performance (P<0.001).

Conclusion

We conducted a correlation analysis of CC performance and the quality of medical chart documentation in a simulation setting. Our results suggest that medical chart documentation can be one possible alternative component in the OSCE.
Psoriatic arthritis screening: A systematic literature review and experts’ recommendations
Authors: Urruticoechea-Arana Ana , Benavent Diego , León Fernando , Almodovar Raquel , Belinchón Isabel , de la Cueva Pablo , Fernández-Carballido Cristina , Loza Estíbaliz , Gratacós Jordi ,   Cribado Working Group
Published: March 2021

Objective

To analyze the performance of psoriatic arthritis (PsA) screening tools, examine their implementation in daily practice, and reach a consensus about the best screening tool for implementation in daily practice in different medical settings.

Methods

A systematic literature review (SLR), structured telephone interviews to hospitals, and a multidisciplinary nominal group meeting were all conducted. The SLR employed sensitive search strategies using Medline, Embase, and the Cochrane Library up to January 2020. Two reviewers independently selected articles that reported data on PsA screening tools and that included sufficient data to at least calculate the sensitivity and specificity of those tools (e.g., questionnaires, algorithms, specific questions, and biomarkers). The hospital interviews collected data regarding the process of suspected PsA diagnosis and referral to rheumatology, the implementation of PsA screening tools, and barriers and facilitators to implementation of those tools. In the nominal group meeting, a multidisciplinary team of experts discussed all these data and subsequently recommended a screening tool for implementation.

Results

The SLR included 41 moderate-quality studies that analyzed 14 PsA screening tools, most of which were questionnaire-based tools. All of these studies reported a moderate-good performance but presented different characteristics regarding the time to completion or the number and type of items or questions. The implementation of screening tools was low (30.5%). The experts ultimately recommended regular use of a PsA screening tool, preferably the PURE-4 questionnaire.

Conclusions

The implementation of PsA screening tools like the PURE-4 questionnaire in daily practice likely improves the prognosis of PsA patients.
Different renoprotective effects of luseogliflozin depend on the renal function at the baseline in patients with type 2 diabetes: A retrospective study during 12 months before and after initiation
Authors: Ito Hiroyuki , Matsumoto Suzuko , Izutsu Takuma , Kusano Eiji , Kondo Jiro , Inoue Hideyuki , Antoku Shinichi , Yamasaki Tomoko , Mori Toshiko , Togane Michiko
Published: March 2021

Aims

The safety and efficacy, particularly, the factors associated with the renal prognosis, were assessed over 12 months after the initiation of luseogliflozin therapy in Japanese patients with type 2 diabetes and renal impairment.

Methods

In total, 238 patients treated with luseogliflozin (2.5 mg, once daily) were studied as the safety analysis set. Two hundred and two subjects whose medication was continued over 12 months were investigated as the full analysis set. The subjects were divided into 3 groups based on the estimated glomerular filtration rate (eGFR): high eGFR (n= 49), normal eGFR (n= 116) and low eGFR (n= 37) groups.

Results

The body weight, systolic blood pressure, HbA1c and urinary protein excretion gradually decreased from baseline in all eGFR groups. While the eGFR was significantly reduced from baseline in the high and normal eGFR groups, the eGFR did not significantly differ over time in the low eGFR group. There was no marked difference in the frequency of adverse events that were specific for SGLT2 inhibitors among the 3 groups in the safety analysis set.

Conclusions

Luseogliflozin can preserve the renal function in the medium term in patients with type 2 diabetes and renal impairment without an increase in specific adverse events.
An image segmentation technique with statistical strategies for pesticide efficacy assessment
Authors: Kim Steven B. , Kim Dong Sub , Mo Xiaoming
Published: March 2021

Image analysis is a useful technique to evaluate the efficacy of a treatment for weed control. In this study, we address two practical challenges in the image analysis. First, it is challenging to accurately quantify the efficacy of a treatment when an entire experimental unit is not affected by the treatment. Second, RGB codes, which can be used to identify weed growth in the image analysis, may not be stable due to various surrounding factors, human errors, and unknown reasons. To address the former challenge, the technique of image segmentation is considered. To address the latter challenge, the proportion of weed area is adjusted under a beta regression model. The beta regression is a useful statistical method when the outcome variable (proportion) ranges between zero and one. In this study, we attempt to accurately evaluate the efficacy of a 35% hydrogen peroxide (HP). The image segmentation was applied to separate two zones, where the HP was directly applied (gray zone) and its surroundings (nongray zone). The weed growth was monitored for five days after the treatment, and the beta regression was implemented to compare the weed growth between the gray zone and the control group and between the nongray zone and the control group. The estimated treatment effect was substantially different after the implementation of image segmentation and the adjustment of green area.
Innate immune signatures to a partially-efficacious HIV vaccine predict correlates of HIV-1 infection risk
Authors: Andersen-Nissen Erica , Fiore-Gartland Andrew , Ballweber Fleming Lamar , Carpp Lindsay N. , Naidoo Anneta F. , Harper Michael S. , Voillet Valentin , Grunenberg Nicole , Laher Fatima , Innes Craig , Bekker Linda-Gail , Kublin James G. , Huang Ying , Ferrari Guido , Tomaras Georgia D. , Gray Glenda , Gilbert Peter B. , McElrath M. Juliana
Published: March 2021

The pox-protein regimen tested in the RV144 trial is the only vaccine strategy demonstrated to prevent HIV-1 infection. Subsequent analyses identified antibody and cellular immune responses as correlates of risk (CoRs) for HIV infection. Early predictors of these CoRs could provide insight into vaccine-induced protection and guide efforts to enhance vaccine efficacy. Using specimens from a phase 1b trial of the RV144 regimen in HIV-1-uninfected South Africans (HVTN 097), we profiled innate responses to the first ALVAC-HIV immunization. PBMC transcriptional responses peaked 1 day post-vaccination. Type I and II interferon signaling pathways were activated, as were innate pathways critical for adaptive immune priming. We then identified two innate immune transcriptional signatures strongly associated with adaptive immune CoR after completion of the 4-dose regimen. Day 1 signatures were positively associated with antibody-dependent cellular cytotoxicity and phagocytosis activity at Month 6.5. Conversely, a signature present on Days 3 and 7 was inversely associated with Env-specific CD4+ T cell responses at Months 6.5 and 12; rapid resolution of this signature was associated with higher Env-specific CD4+ T-cell responses. These are the first-reported early immune biomarkers of vaccine-induced responses associated with HIV-1 acquisition risk in humans and suggest hypotheses to improve HIV-1 vaccine regimens.
RNF111-facilitated neddylation potentiates cGAS-mediated antiviral innate immune response
Authors: Li Chenhui , Zhang Lele , Qian Dong , Cheng Mingxing , Hu Haiyang , Hong Ze , Cui Ye , Yu Huansha , Wang Quanyi , Zhu Juanjuan , Meng Wei , Xu Jin-fu , Sun Yi , Zhang Peng , Wang Chen
Published: March 2021

The cytosolic DNA sensor cyclic GMP-AMP (cGAMP) synthetase (cGAS) has emerged as a fundamental component fueling the anti-pathogen immunity. Because of its pivotal role in initiating innate immune response, the activity of cGAS must be tightly fine-tuned to maintain immune homeostasis in antiviral response. Here, we reported that neddylation modification was indispensable for appropriate cGAS-STING signaling activation. Blocking neddylation pathway using neddylation inhibitor MLN4924 substantially impaired the induction of type I interferon and proinflammatory cytokines, which was selectively dependent on Nedd8 E2 enzyme Ube2m. We further found that deficiency of the Nedd8 E3 ligaseRnf111greatly attenuated DNA-triggered cGAS activation while not affecting cGAMP induced activation of STING, demonstrating that Rnf111 was the Nedd8 E3 ligase of cGAS. By performing mass spectrometry, we identified Lys231 and Lys421 as essential neddylation sites in human cGAS. Mechanistically, Rnf111 interacted with and polyneddylated cGAS, which in turn promoted its dimerization and enhanced the DNA-binding ability, leading to proper cGAS-STING pathway activation. In the same line, theUbe2morRnf111deficiency mice exhibited severe defects in innate immune response and were susceptible to HSV-1 infection. Collectively, our study uncovered a vital role of the Ube2m-Rnf111 neddylation axis in promoting the activity of the cGAS-STING pathway and highlighted the importance of neddylation modification in antiviral defense.
Genomics of an endemic cystic fibrosis Burkholderia multivorans strain reveals low within-patient evolution but high between-patient diversity
Authors: Lood Cédric , Peeters Charlotte , Lamy-Besnier Quentin , Wagemans Jeroen , De Vos Daniel , Proesmans Marijke , Pirnay Jean-Paul , Echahidi Fedoua , Piérard Denis , Thimmesch Matthieu , Boeras Anca , Lagrou Katrien , De Canck Evelien , De Wachter Elke , van Noort Vera , Lavigne Rob , Vandamme Peter
Published: March 2021

Burkholderia multivorans is a member of the Burkholderia cepacia complex (Bcc), notorious for its pathogenicity in persons with cystic fibrosis. Epidemiological surveillance suggests that patients predominantly acquire B. multivorans from environmental sources, with rare cases of patient-to-patient transmission. Here we report on the genomic analysis of thirteen isolates from an endemic B. multivorans strain infecting four cystic fibrosis patients treated in different pediatric cystic fibrosis centers in Belgium, with no evidence of cross-infection. All isolates share an identical sequence type (ST-742) but whole genome analysis shows that they exhibit peculiar patterns of genomic diversity between patients. By combining short and long reads sequencing technologies, we highlight key differences in terms of small nucleotide polymorphisms indicative of low rates of adaptive evolution within patient, and well-defined, hundred kbps-long segments of high enrichment in mutations between patients. In addition, we observed large structural genomic variations amongst the isolates which revealed different plasmid contents, active roles for transposase IS3 and IS5 in the deactivation of genes, and mobile prophage elements. Our study shows limited within-patient B. multivorans evolution and high between-patient strain diversity, indicating that an environmental microdiverse reservoir must be present for this endemic strain, in which active diversification is taking place. Furthermore, our analysis also reveals a set of 30 parallel adaptations across multiple patients, indicating that the specific genomic background of a given strain may dictate the route of adaptation within the cystic fibrosis lung.
Plato’s Intellectual Development and Visual Thinking
Authors: Saracco Susanna
Published: March 2021

Plato has devised texts which call the readers to collaborate cognitively with them. An important epistemic stimulation is the schematization, the line segment, which summarizes Plato’s idea of intellectual development. In this research, visual thinking will help us to make the most of the Platonic invitation to investigate further cognitive growth. It will be analyzed how visual discoveries are rendered possible by mental number lines, realizing the epistemological importance of visualization. Thanks to visualization, structuralism will be grasped. It will reveal a connection with Plato’s philosophy which suggests a novel elaboration of the Platonic concept of intellectual growth.
Semblables inférieures : quels lieux pour les femmes dans la cité juste de Platon ?
Authors: Helmer Etienne
Published: March 2021

What place can women have in Plato’s just city? In opposition to the two main antagonistic positions on this topic - some consider Plato a promoter of gender equality as he allows women to have political office, while others put the stress on the fact that Plato keeps them in a subordinate status - this article makes a new claim: these two positions must be held together because of the nature of the rationality at work in Plato’s political philosophy, as a combination of emancipatory theoretical elements, and the taking into account of the constraints of history.
Mesa dos Sentidos e Sentidos da Mesa: Vol. I
Authors: Soares Carmen , Silveira Anny Jackeline Torres , Laurioux Bruno
Published: March 2021

A presente obra está organizada em dois volumes, contendo seis partes e um total de 37 capítulos.Os 16 capítulos que compõem o volume I estruturam-se em torno de três temáticas centrais. A perceção sensorial de alimentos e ambientes é tratada na Parte I (Sentidos à Mesa). A relação harmónica ou conflituosa entre alimentação e saúde discute-se na Parte II (Mesas terapêuticas, mesas saudáveis e mesas malsãs). Os condicionalismos de ordem gustativa, biológica ou económica por detrás daalimentação servem de pilar aos estudos da Parte III (Mesas de delícias, mesas de sobrevivência e mesas económicas).
VITALISMO FILOSÓFICO EM NIETZSCHE: FUNDAMENTOS EPISTEMOLÓGICOS E A VALORIZAÇÃO DA ARTE NA EDUCAÇÃO
Authors: Hecktheuer Fabio , Lopes Lenir , Lopes Dettoni Josenir
Published: March 2021

Esta pesquisa busca responder à questão da possível relação entre o posicionamento epistemológico de Nietzsche e o papel da Arte na Educação. Para isso, estudamos os fundamentos epistemológicos da filosofia nietzscheana, em especial a temática relativa à verdade, à crítica à supervalorização da razão e a uma sabedoria a serviço da vida. Em seguida, examinamos as consequências educacionais de um pensamento vitalista. Metodologicamente, realizamos pesquisa bibliográfica, investigando relevantes obras de Nietzche sobre o tema, bem como de comentadores de sua filosofia. Na composição do texto, organizamos seis seções: Introdução; A questão da verdade em Nietzsche; Filosofia tradicional e supervalorização da razão; Uma filosofia a serviço da vida; Implicações Educacionais; e Considerações Finais. Com a pesquisa, pudemos concluir que a Arte tem papel essencial na Educação, inspirada no vitalismo nietzschiano, o que nos interpela acerca do lugar que como sociedade temos dado à dimensão estética no âmbito educacional contemporâneo.
POR UMA EDUCAÇÃO FÍSICA ESCOLAR NÃO EXCLUDENTE: DESCONTRUINDO PADRÕES CORPORAIS HEGEMÔNICOS NA ESCOLA
Authors: Longo Monique Marques
Published: March 2021

A escola como instituição responsável pela inserção das crianças na sociedade, desde sua constituição, mostrou-se excludente. Por meio de símbolos, hábitos e discursos, instaura modelos e hierarquiza sujeitos, muitas vezes, utilizando-se das práticas corporais. Este artigo objetivou refletir as possibilidades práticas dos estudos interculturais como referencial teórico para aulas de Educação Física que se pretendem afirmadoras das diferenças identitárias e de práticas corporais não excludentes. Canen (2000, 2002, 2007), Candau (2008), Louro (2008), Sctrazzacaappa (2001) e Csordas (2008) constituíram-se aportes teóricos. Ratificou-se que a abrangência semântica abarcada pelo conceito de corpo desafia pesquisadores mas, também, pode constituir-se em uma possível estratégia subversora de um estereótipo estético etnocentricamente determinado e que categorias como a crítica cultural, a hibridização e a ancoragem social dos conteúdos mostraram-se aportes teóricos potentes aos professores que se pretendem mediadores de aulas de Educação Física não excludentes.
MONITORAMENTO E AVALIAÇÃO DOS PLANOS DECENAIS DE EDUCAÇÃO: UMA ANÁLISE CONCEITUAL-LEGAL
Authors: Militão Silvio Cesar Nunes , Aranda Maria Alice De Miranda
Published: March 2021

Constituído como parte integrante de uma pesquisa mais ampla desenvolvida em nível de pós-doutoramento, o presente artigo tem como objetivo precípuo analisar conceitual e legalmente os termos monitoramento e avaliação e cotejar como os mesmos figuram nos Planos Nacionais de Educação (PNEs) aprovados no corrente século. De abordagem qualitativa, o trabalho utiliza-se de revisão bibliográfica e pesquisa documental acerca da temática em tela. Os resultados evidenciam que, apesar do monitoramento e da avaliação contemporaneamente assumirem maior proeminência no ciclo das políticas públicas e ocuparem lugar de maior destaque no atual PNE e nos correspondentes planos subnacionais aprovados sob sua vigência, este par indissociável somente se efetivará se houver ampla e permanente mobilização e participação da sociedade civil e política em prol da constituição de tais planos como epicentro das políticas educacionais em todos os níveis (nacional, estadual, distrital e municipal).
NOVOS PARADIGMAS: APROXIMAÇÕES ENTRE PÓS-DRAMÁTICO E EDUCAÇÃO EMERGENTE EM UMA PEDAGOGIA TEATRAL
Authors: Sommer Márcia Regina Ribeiro Gomes , Batista Mateus Schimith , Pinho Maria José de
Published: March 2021

Diante das dificuldades para se trabalhar as noções do teatro pós-dramático no ambiente escolar, reconhece-se a emergência de um novo paradigma educacional também na pedagogia teatral. Este trabalho busca perceber aspectos metodológicos influenciados pela relação entre o ensino do teatro pós-dramático e o paradigma educacional emergente, refletindo a partir da concepção de pós-dramático desenvolvido por Lehmann (2007) e de paradigma educacional emergente proposta por Moraes (2007). O estudo utiliza a revisão teórica para delimitar as noções e estabelecer as aproximações e seu possível reflexo no ensino do teatro pós-dramático em uma perspectiva educacional emergente.
PROFESSORES E GESTORES COMO PESQUISADORES: A REFLEXÃO SOBRE A PRÁTICA A PARTIR DA ESCUTA DOS ALUNOS
Authors: Almeida Julio Gomes , Diniz Priscila Martins
Published: March 2021

Este artigo tem como objetivo apresentar reflexões de professores e gestores de escolas de educação básica que participaram, na condição de pesquisadores, da pesquisa Autoavaliação Institucional: a percepção de alunos do ensino fundamental sobre a educação de qualidade, realizada entre 2016 e 2018. A pesquisa envolveu quatro escolas públicas, sendo duas da rede municipal e duas da rede estadual, todas situadas no município de São Paulo. Os dados aqui reunidos decorrem da análise de documentos gerados no processo de pesquisa: atas de encontros mensais e registros de encontros realizados para avaliar a aplicação dos instrumentos de coleta de dados e avaliação final do projeto. Como resultado, é possível afirmar que o desenvolvimento de pesquisa na escola pública com a participação de professores e gestores na condição de pesquisadores favorece a aquisição de conhecimento no domínio da teoria e promove o exercício de utilização deste conhecimento no domínio da prática. Além disso, promove a aproximação entre professores e alunos, dos professores entre si e da universidade com a escola.
Characterization of Some Commercially Important Octopus (Mollusca: Cephalopoda) from Indonesian Waters using Mitochondrial DNA Cytochrome Oxidase Sub-Unit I (Mt-DNA COI)
Authors: Kholilah Nenik , Afiati Norma , Subagiyo Subagiyo
Published: March 2021

As per the FAO data, octopus identification is very limited in the species level at world fishery and also they are cryptic nature. On the other hand, Indonesia is one of the top ten highest octopus exporters. This study therefore aimed to determine the species of octopus based on phylogenetic analysis of mt-DNA COI. Octopuses were collected from nine different locations throughout Indonesia, i.e., Anambas, Bangka-Belitung, Cirebon, Karimunjawa, Tuban, Lombok, Buton, Wakatobi and Jayapura. Samples were mostly in the form of tentacles that were directly collected from fishermen. After being preserved in 96% ethanol, the sample was extracted in 10% chelexÒ, PCR amplification using Folmer’s primer then was further analysed by sequencing in Sanger methods. Of the 24 samples sequenced, the results recognized four species Octopodidae belongs to the three genera, named Amphioctopus aegina, Hapalochlaena fasciata, Octopus laqueus and Octopus cyanea. Mean pair-wise distances of within-species were ranged from 0 to 5.5 % and between-species was ranged from 12.9 to 15.8 %. This study distinctly confirmed the difference between genus Amphioctopus and Hapalochlaena (15.5 %), as also between O. laqueus and O. cyanea (12.9%) which was previously not completely distinguished. Although performing species identification using DNA sequences for shallow-water benthic octopus species is perhaps considered premature, this study indicated the possible application of COI sequences for species identification, thereby providing a preliminary dataset for future DNA barcoding of octopus, in particular for Indonesia waters.
Application of Offshore HDPE Pipes Route Design in North Maluku Indonesia
Authors: Novico Franto , Kurniawan Indra , Egon Andi , Merli Davide
Published: March 2021

The lack of fresh water for the inhabitants of Maitara island is a very urgent problem to be solved. Two main factors at least must be taken into account to deliberate the right of way of subsea High-density polyethylene (HDPE) pipes, namely the hydrodynamic conditions and of a block analysis. This paper presents the study to justify the best route of subsea HDPE pipes based on hydrodynamic model analysis and concrete block strategy. The method used to analyze the best route includes 2 aspects. Firstly, the investigation method consisting of a bathymetric survey conducted by a single beam echosounder, 15 days tidal observations and seabed sediment sampling. Secondly, the hydrodynamic modelling analysis using Mike 21 FMHD and concrete block analysis, all these studies have been completed in August 2018. In the morphological behaviour analysis, three alternative routes are considered for the subsea HDPE pipes from Tidore Island to Maitara Island. The outcome of the analysis shows that the second track line option has the smallest impact by the hydrodynamic conditions, with a current speed of less than 0,5m/sec and a significant wave height of fewer than 1.2 meters. Furthermore, the uniformity of the lithology along the route is the other reason to select the second route. Finally, the concrete block analysis generated a minimum dimension of 75cm x 60cm x 30cm, and a free span of 3 meters is safe to absorb the uplift and drag forces acting on the pipe.
Opieka nad sierotami we wczesnonowożytnym Kleczewie
Authors: Hajdrych Łukasz
Published: March 2021

The care of orphans was one of the main concerns of early modern magistrates across the whole Europe. In each country and town this care could take a completely different form, ranging from placing parentless children in asylums to assigning them to certain families. This paper deals with the problem of the orphan-care in a small town of the Great Poland region in 17th and 18th centuries, on the example of private town of Kleczew, located in the east part of the region.
170 lat realizacji systemu ochrony przez wychowanie w koncepcji Edmunda Bojanowskiego
Authors: Opiela S. Maria
Published: March 2021

The pedagogical thought and concept of the system of protection by Edmund Bojanowski’s upbringing, even though it was born 170 years ago, appears today to be innovative and exceptionally up-to-date. Applied in contemporary pre-school education and child care, it is a continuation of the concept of upbringing and its practical implementation by establishing on May 3, 1850 the nursery and Congregation of the Servant Sisters. The theoretical and practical application of this concept and new research and scientific studies on the essence of early education realized in Catholic pedagogical thought in the context of contemporary civilization challenges is still expected from various circles in Poland and worldwide.
Alyson L. Lavigne, Thomas L. Good, Enhancing Teacher Education, Development, and Evaluation. Lessons Learned from Educational Reform. New York 2019: Routlege, pp. 194
Authors: Adamczewski Jakub
Published: March 2021

Review
Edukacja akademicka kobiet w przekazie prasy społeczno-kulturalnej i pedagogicznej Królestwa Polskiego na przełomie XIX i XX wieku
Authors: Krakowiak Małgorzata
Published: March 2021

The purpose of the article is to present a journalistic description of the issue of academic education of women in selected periodicals of the Kingdom of Poland in the years 1894–1914. The issue of women’s academic education was described in various ways, which depended on the profile of individual periodicals. The publications had mainly an opinion-forming and informative function. Critical opinions were published in the conservative press, they showed study abroad as a danger. Progressive magazines described women’s studies as a chance for girls development. Periodicals were a source of information about the number of women undertaking academic education abroad, selected faculties and student societies. The frequency of publications depended on legislative changes and events related to academic life that aroused public and public interest. This article looked for answers to the question of how the issue of academic education of women from the Kingdom of Poland was problematized in selected press? In the article uses the methods of historical and pedagogical research and press research (text analysis). Research on the journalistic description of women’s academic education should be continued based on other categories of periodicals, including women’s press.
Stanisław Keturakis – Litwin w służbie rosyjskiej oświaty na terenie guberni piotrkowskiej w latach 1895–1914
Authors: Złotkowski Dariusz
Published: March 2021

At the end of the 19th century, the teaching profession was the aspiration of many peasant sons. The position of a teacher ensured a modest but quite stable income. A Lithuanian, born in 1989, Stanislaw Keturakis, was one of graduates of the Teachers’ College in Wejwery near Kaunas. This institution offered a state scholarship. In return for this financial help, its graduates had to accept posts in primary schools determined by educational authorities. A few graduates of this school, mostly Lithuanians, were sent to work in village schools of Piotrków province. One of them, Stanislaw Keturakis, began his first teaching job in a school in Jedlno. He was confronted with difficult living conditions, the school was only planned to be built. At this time, he married Józefa Birsztejn and they had two sons: Eugeniusz Józef (1901) and Zdzisław Aleksander (1904). Peasants perceived teachers as tsarist officials. In 1901, S. Keturakis was transferred to Mstów, to work as a teacher, then to Wancerzów, and again back to Jedlno. Taking over a position of a teacher in Zagórze (1907) was clearly a promotion. The school belonged to the private property of Grand Duke Michael Alexandrovich of Russia, a brother of Emperor Nicholas II. The last stage of S. Keturakis’s teaching career was his work in a school in Zagórze. Working there in the years 1907–1914, he taught Russian, Polish, Arithmetic, History and Geography. At the end of the summer 1914, he got an opportunity to take over the post of the forester’s assistant in Orłow province, but the outbreak of the war made it impossible. Lack of any sources does not allow us to determine what the further life of Stanislaw Keturakis was like.
Stereotyp w przestrzeni symbolicznej
Authors: Hendrykowski Marek
Published: March 2021

The subject of the article is stereotypes considered in the context of symbolic space. This redefinition and reconsideration of the complex issue of stereotypes and stereotyping of diferent kinds in art and everyday life aims at demonstrating the operational usefulness of this concept in contemporary humanistic re-ection, including the sphere of art, everyday life and various fields of scientific research.
„Zamknij drzwi”, czyli wykorzystanie przestrzeni filmowej do uobecnienia sacrum na przykładzie filmu Sieranevada Cristi Puiu
Authors: Przybylski Krystian
Published: March 2021

The article analyzes how the main representative of the Romanian New Wave – Cristi Puiu – uses the visual space of an apartment for staging and presenting the visible and hidden spheres of reality. His film Sieranevada, focusing on a family meeting on the occasion of religious ceremonies commemorating recently deceased senior family member, shows the possibilities of using the language of realism to appeal to the spirituality present in everyday life and to unveil Transcendence.
Między minimalizmem a melodramatem. Przestrzeń filmowa w kinie rumuńskiej nowej fali
Authors: Domalewski Adam
Published: March 2021

The starting point for considering the means of creating and designing a film space in Romanian New Wave cinema is the thesis that Romanian film art in the 21st century is an artistic response to the times of communism and that images of the post-socialist architectural space – their multi-faceted functionalization in film mise-en-scéne – are the key method of evoking memories of the past. The article discusses, via the example of movies by Crisitian Mungiu, Corneliu Porumboiu and Cristi Puiu, (1) ways of depicting the “closing” of film characters in the cramped interiors of their apartments and (2) film images of individuals’ alienation in public spaces, both of them allusively referring to the times of the oppressive Nicolae Ceaușescu regime. The most important cinematographic devices used bythe authors of the Romanian New Wave cinema include: seemingly static shots, which, however, are filmed with a camera that is constantly gently moving and observing, and tableau shots. A common feature of all the considered works is the panopticism of the film world: the private and public spaces designed in them easily become a place of constant, panoptical observation. In the summary, theauthor remarks on and briefly discusses the coexistence of the aesthetics of minimalism / realism with the melodramatic elements in the cinematic structure of Romanian New Wave films.
Functions of screen space in shots by Mieczysław Jahoda in the example of his first feature films: Zimowy zmierzch and Pętla
Authors: Maron Marcin
Published: March 2021

The text is devoted to discussing the formation methods and the functions of screen space in shots by Mieczysław Jahoda in the films Zimowy zmierzch (directed by S. Lenartowicz) and Pętla (directed by W.J. Has). Mieczysław Jahoda is presented as one of the main initiators of stylistic changes in the films of Polish School in the mid 1950s. The analysis concerns the camera means applied by Jahoda in order to obtain screen effects: light, frame composition, photographic optics and perspective transformation. The film shots by Mieczysław Jahoda are characterized by an exceptional ability to evoke mental space, emotions, memory and imagination via the shapes of screen space. Their feature is a special esthetization aimed at creating the atmosphere of films, as well as symbolic and cultural references.
Przestrzeń poza kadrem – o sensualnym wymiarze zdjęć filmowych Emmanuela Lubezkiego
Authors: Pękala Iga
Published: March 2021

The article presents an analysis of the sensual potential of film images via the example of cooperation between operator Emmanuel Lubezki and three directors: Alejandro Gonzales Iñárritu, Alfonso Cuarón and Terrence Malick. The impact of reconstruction of film space on the sensual impressions of viewers in the context of the American theory of somatic cinema was also analyzed. What influence does the dimension of cinematographic cooperation have on the concept of authorial cinema?
Boks w kadrze. Wybrane strategie inscenizacyjne w polskich filmach o boksie z lat sześćdziesiątych i siedemdziesiątych XX wieku
Authors: Wierski Dominik
Published: March 2021

In his article, Dominik Wierski analyses and interprets selected staging strategies in Polish films about boxing. The films cited in the text were made in the 1960s and 1970s. It was a dynamic period in Polish cinematography, but also a great time for Polish boxing. The author wonders how filmmakers showed space related to boxing - training rooms, changing rooms, stands and the boxing ring. Selected films, belonging to the most important examples of Polish filmmakers’ insight into the world of boxing, are analyzed in the terms of their use of colors, sound, camera travel, rhythm and pace etc. The purpose of the text is to answer the question of whether Polish films of the ‘60s and ‘70s developed an integral way of discussing boxing and showing its most characteristic spaces.
Kolor pieniędzy. Filmowy styl Michaela Manna
Authors: Sitek Wojciech
Published: March 2021

According to film studies experts, Michael Mann’s movies has the look and the smell of reality. The polisensoric character of his works is used to exhibit the aura of non-screen reality. Michael Mann uses narrative and stylistic elements to show the destructive side of social inequalities. In his genre films, he is presenting themes of social injustice and toxic corporate system (which are often hidden behind the elegant façade of late capitalism’s aesthetics).
Andrzej Stasiuk, tekstowe figury doświadczenia
Authors: Kloch Zbigniew
Published: March 2021

The aim of the article is to show the role of various metaphorical practices that are a dominant structural feature of Andrzej Stasiuk’s prose, used to construct his textual reality. Comparisons, metaphorical statements, and enumerations represent textual figures that transform the described experience of the world from the perspective of the speaking subject, subjectivising and constructing reality, rather than merely representing it. In both his novels and feuilletons, Stasiuk relates his experiences of the world in different temporal orders, constructing them on a textual level. Yet his thoroughly subjective prose preserves the conventions of realism. At the same time, Stasiuk quite often refers to the genre of the road novel, both in his fiction and in his journalism. Textual figures of experience are his basic means of describing the presented world.
Marsjanie. Borowczyk i Lenica na Międzynarodowym Konkursie Filmu Eksperymentalnego w Brukseli
Authors: Sitkiewicz Paweł
Published: March 2021

The text describes the backstage of the legendary International Experimental Film Competition in Brussels, which accompanied the Expo-58 Exhibition at a turning point of the political thaw in Eastern and Central Europe. The most important representatives of avant-garde cinema and auteur animation of three generations took part in this event. The Grand Prix was awarded to The House of Walerian Borowczyk and Jan Lenica. The prize became the ticket to a European career for these two young filmmakers. The competition was also remembered as a symbolic beginning of the “Polish school of animation” (or the so-called “Polish school of experimental film”) on the global film market. The author of the article also writes about a difficult return to Poland, about a cash prize that caused many problems, about the successes of Polish films in Brussels, and about the reception of The House in Poland and abroad. Finally, he tries to demonstrate why this bleak and difficult to understand film, which builds opposition to the optimism of Expo Exhibitions, won the main prize, beating over 300 competitors from around the world.
Regular Bernstein blocks
Authors: Adler Jeffrey D. , Mishra Manish
Published: March 2021

For a connected
reductive group G defined over a non-archimedean local field F,
we consider the Bernstein blocks in the category of smooth representations
of

G
⁢

(
F
)

{G(F)}

.
Bernstein blocks whose cuspidal support involves a regular supercuspidal
representation are called regular Bernstein blocks.
Most Bernstein
blocks are regular when the residual characteristic of F
is not too small.
Under mild hypotheses on the residual characteristic,
we show that the Bernstein
center of a regular Bernstein block of

G
⁢

(
F
)

{G(F)}

is isomorphic to the Bernstein center of a regular depth-zero Bernstein block
of

G
0

⁢

(
F
)

{G^{0}(F)}

, where

G
0

{G^{0}}

is a certain twisted Levi subgroup of
G.
In some cases, we show that the blocks themselves are equivalent,
and as a consequence we prove the ABPS Conjecture in some new cases.
A new Approach for surveying the axis of cylindrical pipes using a reflectorless total station
Authors: Faried Y. A.
Published: March 2021

To survey the pipe’s axis used in the installation of the cable-stayed bridge, a reflectorless total station and a proposed surveying observation technique were used. The approach includes a new method for observation and a graphical solution for the pipe’s axis determination. The technique shown in this paper can be applied for vertical and semi-vertical cylindrical pipes. A field test with real data and results is also presented.
Novel perspectives for SARS-CoV-2 genome browsing
Authors: Gültekin Visam , Allmer Jens
Published: March 2021

SARS-CoV-2 has spread worldwide and caused social, economic, and health turmoil. The first genome assembly of SARS-CoV-2 was produced in Wuhan, and it is widely used as a reference. Subsequently, more than a hundred additional SARS-CoV-2 genomes have been sequenced. While the genomes appear to be mostly identical, there are variations. Therefore, an alignment of all available genomes and the derived consensus sequence could be used as a reference, better serving the science community. Variations are significant, but representing them in a genome browser can become, especially if their sequences are largely identical. Here we summarize the variation in one track. Other information not currently found in genome browsers for SARS-CoV-2, such as predicted miRNAs and predicted TRS as well as secondary structure information, were also added as tracks to the consensus genome. We believe that a genome browser based on the consensus sequence is better suited when considering worldwide effects and can become a valuable resource in the combating of COVID-19. The genome browser is available at http://cov.iaba.online.
Variable Selection in Regression Models Using Global Sensitivity Analysis
Authors: Becker William , Paruolo Paolo , Saltelli Andrea
Published: March 2021

Global sensitivity analysis is primarily used to investigate the effects of uncertainties in the input variables of physical models on the model output. This work investigates the use of global sensitivity analysis tools in the context of variable selection in regression models. Specifically, a global sensitivity measure is applied to a criterion of model fit, hence defining a ranking of regressors by importance; a testing sequence based on the ‘Pantula-principle’ is then applied to the corresponding nested submodels, obtaining a novel model-selection method. The approach is demonstrated on a growth regression case study, and on a number of simulation experiments, and it is found competitive with existing approaches to variable selection.