bluuebunny/crossref_metadata_2025 huggingface dataset:
“Wait, I can’t do that anymore!”: pandemic teacher immediacy in college communication classes
Authors: Chatham-Carpenter April , Malone Michelle
Published: September 2023

Introduction

The stress and strain due to various aspects of the pandemic affected teaching and learning. Relating between instructors and students, and between students, may never be the same. Adjustments to teaching and learning may still need to be made due to the lingering effects of the pandemic, especially as zoom classrooms continue to be used within communication and other disciplines.

Methods

In this study, the researchers interviewed 15 communication instructors, using indepth semi-structured zoom interviews, about their experiences in the pandemic classroom and how they attempted to build relationships and connections with students during the COVID-19 pandemic.

Results

The researchers found specific immediacy strategies used by instructors, with participants indicating they attempted to use the more traditional teacher nonverbal immediacy behaviors such as eye contact, facial expressions, physical distance, and touch, but were hampered by the wearing of masks, practicing social distancing, and moving to online teaching modalities where student engagement was limited at best. Instructors also adapted verbal immediacy behaviors, as they used various strategies for inviting participation, providing feedback, and being real to develop connections with students, as well as building in specific teaching structures into their pandemic classrooms. In addition, participants indicated they used a variety of additional immediacy-related strategies and behaviors to build relationships once moving to blended HyFlex or online teaching. These strategies were used consistently, as instructors seamlessly moved between the online synchronous classroom, the blended classroom, and the face-to-face classroom with masking and physical distancing required.

Discussion

Our research revealed that there were unique ways relationships were built, typically using different types of media to enact teacher immediacy in nuanced ways. We argue that looking at such teaching using both media multiplexity and embodied pedagogy perspectives can enhance the teacher immediacy literature by demonstrating how teacher immediacy was changed during the pandemic, as media richness increased the likelihood of developing relationships between teachers and students through an embodied pedagogy of caring using technological tools.
Radiomics optimizing the evaluation of endometrial receptivity for women with unexplained recurrent pregnancy loss
Authors: Huang Wendi , Jin Yi , Jiang Lulu , Liang Mengjie
Published: September 2023

Background

The optimization of endometrial receptivity (ER) through individualized therapies has been shown to enhance the likelihood of successful gestation. However, current practice lacks comprehensive methods for evaluating the ER of patients with recurrent pregnancy loss (RPL). Radiomics, an emerging AI-based technique that enables the extraction of mineable information from medical images, holds potential to offer a more objective and quantitative approach to ER assessment. This innovative tool may facilitate a deeper understanding of the endometrial environment and enable clinicians to optimize ER evaluation in RPL patients.

Objective

This study aimed to identify ultrasound radiomics features associated with ER, with the purpose of predicting successful ongoing pregnancies in RPL patients, and to assess the predictive accuracy of these features against regular ER parameters.

Methods

This retrospective, controlled study involved 262 patients with unexplained RPL and 273 controls with a history of uncomplicated full-term pregnancies. Radiomics features were extracted from ultrasound endometrial segmentation images to derive a radiomics score (rad-score) for each participant. Associations between rad-scores, baseline clinical variables, and sonographic data were evaluated using univariate and multivariate logistic regression analyses to identify potential indicators of RPL. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the predictive accuracy of the rad-score and other identified indicators in discriminating RPL cases. Furthermore, the relationships between age and these identified indicators were assessed via Pearson correlation analysis.

Results

From the 1312 extracted radiomics features, five non-zero coefficient radiomics signatures were identified as significantly associated with RPL, forming the basis of the rad-score. Following multivariate logistic regression analysis, age, spiral artery pulsatility index (SA-PI), vascularisation index (VI), and rad-score emerged as independent correlates of RPL (all P<0.05). ROC curve analyses revealed the superior discriminative capability of the rad-score (AUC=0.882) over age (AUC=0.778), SA-PI (AUC=0.771), and VI (AUC=0.595). There were notable correlations between age and rad-score (r=0.275), VI (r=-0.224), and SA-PI (r=0.211), indicating age-related variations in RPL predictors.

Conclusion

This study revealed a significant association between unexplained RPL and elevated endometrial rad-scores during the WOI. Furthermore, it demonstrated the potential of rad-scores as a promising predictive tool for successful ongoing pregnancies in RPL patients.
Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome
Authors: Brennan Edwina , Butler Alexandra E. , Drage Daniel S. , Sathyapalan Thozhukat , Atkin Stephen L.
Published: September 2023

Introduction

Polychlorinated biphenyls (PCBs), organic lipophilic pollutants that accumulate through diet and increase with age, have been associated with polycystic ovary syndrome (PCOS) and shown to affect microRNA (miRNA) expression. This work aimed to determine if PCBs were associated with circulating miRNAs and whether there were any correlations with serum PCB/miRNA levels and hormonal changes.

Methods

29 non-obese PCOS and 29 healthy control women, with similar age and body mass index (BMI), had their serum miRNAs measured together with 7 indicator PCBs (PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, PCB180) using high resolution gas chromatography coupled with high resolution mass spectrometry.

Results

In the combined study cohort, four miRNAs (hsa-miR-139-5p, hsa-miR-424-5p, hsa-miR-195-5p, hsa-miR-335-5p) correlated with PCBs, but none correlated with metabolic parameters. hsa-miR-335-5p correlated with FSH. When stratified, 25 miRNAs correlated with PCBs in controls compared to only one (hsa-miR-193a-5p) in PCOS; none of these miRNAs correlated with the metabolic parameters of BMI, insulin resistance, or inflammation (C-reactive protein, CRP). However, of these 25 miRNAs in controls, hsa-miR-26a-5p, hsa-miR-193a-5p, hsa-miR-2110 and hsa-miR-195-5p positively correlated with luteinizing hormone (LH), hsa-miR-99b-5p and hsa-miR-146b-5p correlated with estradiol, hsa-miR-193a-5p correlated with progesterone, hsa-miR-195-5p correlated with follicle stimulating hormone (FSH), and hsa-miR-139-5p and hsa-miR-146b-5p negatively correlated with anti-müllerian hormone (AMH) (all p<0.05). hsa-miR-193a-5p in PCOS cases correlated with estradiol.

Conclusion

In this cohort of women, with no difference in age and BMI, and with similar PCB levels, the miRNAs correlating to PCBs associated with menstrual cycle factors in healthy menstruating controls versus the anovulatory PCOS subjects. The PCB-associated miRNAs did not correlate with non-reproductive hormonal and metabolic parameters. This suggests that PCB effects on miRNAs may result in changes to the hypothalamo-ovarian axis that may thus affect fertility.
The role of blockchain technology in advancing sustainable energy with security settlement: enhancing security and efficiency in China’s security market
Authors: Weixiong Wang
Published: September 2023

Blockchain technology has the potential to revolutionize securities settlement systems, offering an efficient, reliable, and cost-effective alternative to traditional methods. Its features, including distributed data authenticity, programmability, and scalability, can enhance security and efficiency in China’s securities market, while promoting a sustainable energy future. However, to fully leverage the benefits of blockchain in securities registration and settlement, it is crucial to address algorithmic loopholes and operational risks associated with smart contracts. Establishing blockchain technical standards and rules is also necessary to ensure smooth system operation. Furthermore, given the uncertainty of the final settlement time point, adherence to decentralization principles and the incorporation of embedded technology for supervision are essential. Legislative measures are required to regulate smart contracts and mitigate systemic risk effectively. This will ensure a stable settlement time expectation and enable fair allocation of legal responsibility among the involved parties. Additionally, other regulatory approaches should be implemented to provide effective supervision and adapt to the rapid development of blockchain. By addressing challenges and risks, blockchain’s full potential can be realized, enabling a sustainable energy future, while enhancing security and efficiency in China’s securities market.
Analytical analysis for horizontal displacement of pile anchor support structure considering prestress
Authors: Ling Yong-qiang , Song Lei , Hao Jian , Cao Xiao-lin , Yang Xiao-hui
Published: September 2023

The pile-anchor supporting structure is widely used in foundation pit engineering, it is particularly necessary for calculating lateral displacement of the pile-anchor supporting structure. The soil stress state is transformed by the additional stress caused by the prestress, thus the lateral displacement can be decreased own to pile-anchor supporting structure bearing active earth pressure had changed. Prestress as a concentrated force is decomposed into the horizontal component and vertical component, calculating the additional stress caused by the prestress and getting the formula of active earth pressure of considering the additional stress, setting up calculation model of the pile-anchor supporting structure. For typical examples of foundation pit engineering, the parameters are substituted into a program written based on derived formulas, and the elastic deformation superposition method is used to calculate the horizontal displacement of the pile anchor support structure. The comparison and verification is carried out by Finite element analysis. The influence of cohesion and friction angle on pile displacement, bending moment, and shear force has been studied. This study found that cohesion and friction angle have a significant impact on the displacement of pile anchor support structures.
Identification of biomarkers in patients with rheumatoid arthritis responsive to DMARDs but with progressive bone erosion
Authors: Marasco Emiliano , Fabbriciani Gianluigi , Rotunno Laura , Longhi Matteo , De Luca Paola , de Girolamo Laura , Colombini Alessandra
Published: September 2023

Introduction

Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that may cause joint destruction and disability. The pharmacological treatment of RA aims at obtaining disease remission by effectively ceasing joint inflammation and arresting progressive bone erosions. Some patients present bone lesions accrual even after controlling joint inflammation with current therapies. Our study aimed to analyze lymphocyte subsets and levels of circulating cytokines in patients with RA with progressive bone erosions.

Methods

We enrolled 20 patients with a diagnosis of RA and 12 healthy donors (HD). Patients with RA were divided into patients with bone erosions (RA-BE+) and without bone erosions (RA-BE-). Lymphocyte subsets in peripheral blood were evaluated by flow cytometry. Circulating cytokines levels were evaluated by protein array.

Results

The distribution of lymphocyte subsets was not able to separate HD from AR patients and RA-BE+ and RA-BE- in cluster analysis. We observed a significant expansion of CXCR5- PD1+ T peripheral helper cells (Tph cells) and a reduction in both total memory B cells and switched memory B cells in RA patients compared to HD. We observed an expansion in the frequency of total B cells in RA-BE+ patients compared to RA-BE- patients. Unsupervised hierarchical clustering analysis of 39 cytokines resulted in a fairly good separation of HD from RA patients but not of RA-BE+ patients from RA-BE- patients. RA-BE+ patients showed significantly higher levels of IL-11 and IL-17A than RA-BE- patients.

Conclusion

We show that patients with progressive erosive disease are characterized by abnormalities in B cells and in cytokines with a proven role in bone reabsorption. Understanding the role played by B cells and the cytokine IL-11 and IL-17A in progressive erosive disease can help identify novel biomarkers of erosive disease and design treatment approaches aimed at halting joint damage in RA.
Tracer particle motion driven by vortex formation in the bottom boundary layer underneath internal solitary waves
Authors: Ellevold Thea Josefine , Grue John , Sletten Joakim Soløy
Published: September 2023

Internal solitary waves (ISWs) of large amplitude moving in the coastal ocean induce sizeable horizontal velocities above the sea bed. In turn, these give rise to instability and vortex formation in the bottom boundary layer (BBL), and sediment resuspension and concentration maintenance in the water column. We present two-dimensional laminar simulations in a numerical tank suitable for internal wave motion, including the processes of the BBL. The combined wave and vorticity field encounters a cloud of tracer particles near the bottom. The tracer particles are moved vertically because of the vorticity field during a first encounter. The reflected wave intercepts a second time with the tracer particles, which are then moved further vertically. Numerical experiments with a kinematic viscosity of 1/100 cm2 s−1 or 1/1000 cm2 s-1 are used to manipulate the scale of the Reynolds number at a moderate and great laboratory scale. The final vertical position of the tracer particles is found below a vertical level of approximately 0.23 times the water depth (H) after the second passage. The result is independent of the scale. This vertical position matches available field measurements of a summer benthic nepheloid layer reaching a height of 0.19H. The laminar model predictions compare very well to the ISW-driven vortex formation measured in a three-dimensional laboratory wave tank. Convergence of the calculated vortex formation is documented.
Spatiotemporal variability in ocean-driven basal melting of cold-water cavity ice shelf in Terra Nova Bay, East Antarctica: roles of tide and cavity geometry
Authors: Kim Taekyun , Hong Ji-Seok , Jin Emilia Kyung , Moon Jae-Hong , Song Sang-Keun , Lee Won Sang
Published: September 2023

Mass loss from ice shelves occurs through ocean-driven melting regulated by dynamic and thermodynamic processes in sub-ice shelf cavities. However, the understanding of these oceanic processes is quite limited because of the scant observations under ice shelves. Here, a regional coupled sea-ice/ocean model that includes physical interactions between the ocean and the ice shelf is used as an alternative tool for exploring ocean-driven melting beneath the Nansen Ice Shelf (NIS) which is a cold-water cavity ice shelf located beside Terra Nova Bay (TNB) in East Antarctica. For the first time, this study identifies the spatiotemporal variability signatures for different modes of ocean-driven melting at the base of NIS. In February (austral summer), basal melting substantially increases where the ice shelf draft is relatively small in the vicinity of the ice shelf front, contributing 78% of the total NIS melting rate. As the dominant source of NIS mass loss, this melting is driven by tide-induced turbulent mixing along the sloping ice shelf base and summer warm surface water intruding beneath and reaching the shallow parts of the ice shelf. In contrast, the NIS has relatively high basal melting rates near the grounding line in September (austral winter) primarily because of the intrusion of high-salinity shelf water produced by polynya activity in TNB that flows into the cavity beneath NIS toward the deep grounding line. Of the total melting rate of NIS in winter, 36% comes from regions near the grounding line. In addition, the contributions of tides and realistic cavity geometry to NIS basal melting are identified by conducting sensitivity experiments. Tidal effects increase the melting of NIS throughout the year, particularly contributing as much as 30% to the areas of ice draft shallower than 200 m in summer. Sensitivity results for uncertainty in cavity geometry show that spurious vertical mixing can be locally induced and enhanced by interaction between tides and the unrealistic topography, resulting in excessive basal melting near the NIS frontal band. The sensitivity experiments have shown that tides and realistic cavity geometry bring a significant improvement in the estimation of basal melt rates through a numerical model.
Cyclic axial compressive performance of hybrid double-skin tubular square columns
Authors: Wang Dai , Yuan Jian Song , Li Yunchu , Fu Quanchao , Zhang Hengchang
Published: September 2023

This paper presents an experimental study on the cyclic axial compressive behavior of FRP-concrete-steel hybrid double-skin tubular columns. The square column specimens were cast with an external Fiber Reinforced Polymer jackets, inner steel tube and concrete in between. The height of the columns was 500 mm and the side dimension was 150 mm. The effects of loading scheme, void ratio and diameter-thickness ratio on axial compression behavior were investigated. A total of eight columns were tested under monotonic and cyclic axial compression. The experimental results show that the effect of loading scheme on axial stress-strain envelope curve and the peak load were not significant, and the ultimate state of the square columns subjected to cyclic axial compression was very similar to that of specimens subjected to monotonic axial compression. Besides, compared with void ratio, the diameter-thickness ratio of the inner steel tube has significant influence on the peak load of the columns when subjected to cyclic axial compression.
Early referring saved lives in kidney transplant recipients with COVID-19: a beneficial role of telemedicine
Authors: Zahradka Ivan , Petr Vojtech , Jakubov Katarina , Modos Istvan , Hruby Filip , Viklicky Ondrej
Published: September 2023

Introduction

There is a strong impetus for the use of telemedicine for boosting early detection rates and enabling early treatment and remote monitoring of COVID-19 cases, particularly in chronically ill patients such as kidney transplant recipients (KTRs). However, data regarding the effectiveness of this practice are lacking.

Methods

In this retrospective, observational study with prospective data gathering we analyzed the outcomes of all confirmed COVID-19 cases (n = 955) in KTRs followed at our center between March 1, 2020, and April 30, 2022. Risk factors of COVID-19 related mortality were analyzed with focus on the role of early referral to the transplant center, which enabled early initiation of treatment and remote outpatient management. This proactive approach was dependent on the establishment and use of a telemedicine system, which facilitated patient-physician communication and expedited diagnostics and treatment. The main exposure evaluated was early referral of KTRs to the transplantation center after confirmed or suspected COVID-19 infection. The primary outcome was the association of early referral to the transplantation center with the risk of death within 30 days following a COVID-19 diagnosis, evaluated by logistic regression.

Results

We found that KTRs who referred their illness to the transplant center late had a higher 30-day mortality (4.5 vs. 13.6%, p < 0.001). Thirty days mortality after the diagnosis of COVID-19 was independently associated with late referral to the transplant center (OR 2.08, 95% CI 1.08–3.98, p = 0.027), higher age (OR 1.09, 95% CI 1.05–1.13, p < 0.001), higher body mass index (OR 1.06, 95% CI 1.01–1.12, p = 0.03), and lower eGFR (OR 0.96, 95% CI 0.94–0.98, p < 0.001) in multivariable logistic regression. Furthermore, KTRs who contacted the transplant center late were older, had longer time from transplantation, lived farther from the center and presented with higher Charlson comorbidity index.

Discussion

A well-organized telemedicine program can help to protect KTRs during an infectious disease outbreak by facilitating pro-active management and close surveillance. Furthermore, these results can be likely extrapolated to other vulnerable populations, such as patients with chronic kidney disease, diabetes or autoimmune diseases requiring the use of immunosuppression.
Fecal microbial and metabolic characteristics of swine from birth to market
Authors: He Huan , Yang Mingzhi , Li Wentao , Lu Zeqing , Wang Yizhen , Jin Mingliang
Published: September 2023

Introduction

Recently, the research on pig intestinal microbiota has become a hot topic in the field of animal husbandry. There are few articles describing the dynamic changes of porcine fecal microbiota and metabolites at different time points from birth to market.

Methods

In the present study, 381 fecal samples were collected from 633 commercial pigs at 7 time points, including the 1st day, the 10th day, the 25th day, the 45th day, the 70th day, the 120th day, and the 180th day after the birth of swine, were used for microbiome analysis by Illumina MiSeq sequencing methods while 131 fecal samples from 3 time points, the 10th day, the 25th day, and 70th day after birth, were used for metabolome analysis by LC–MS methods.

Results

For the microbiome analysis, the fecal microbial richness increased over time from day 1 to 180 and the β-diversity of fecal microbiota was separated significantly at different time points. Firmicutes were the main phyla from day 10 to 180, followed by Bacteroides. The abundance of Lactobacillus increased significantly on day 120 compared with the previous 4 time points. From day 120 to day 180, the main porcine fecal microbes were Lactobacillus, Clostridium_sensu_stricto_1, Terrisporobacter and Streptococcus. Clostridium_sensu_stricto_1 and Terrisporobacter increased over time, while Lactobacillus, Escherichia-Shigella, Lachnoclostridium decreased with the time according to the heatmap, which showed the increase or decrease in microbial abundance over time. For the metabolome analysis, the PLS-DA plot could clearly distinguish porcine fecal metabolites on day 10, 25, and 70. The most different metabolic pathways of the 3 time points were Tryptophan metabolism, Sphingolipid signaling pathway, Protein digestion and absorption. Some metabolites increased significantly over time, such as Sucrose, L-Arginine, Indole, 2,3-Pyridinedicarboxylic acid and so on, while D-Maltose, L-2-Aminoadipic acid, 2,6-diaminohexanoic acid, L-Proline were opposite. The correlation between fecal metabolites and microbiota revealed that the microbes with an increasing trend were positively correlated with the metabolites affecting the tryptophan metabolic pathway from the overall trend, while the microbes with a decreasing trend were opposite. In addition, the microbes with an increasing trend were negatively correlated with the metabolites affecting the lysine pathway.

Discussion

In conclusion, this study elucidated the dynamic changes of porcine fecal microbiota and metabolites at different stages from birth to market, which may provide a reference for a comprehensive understanding of the intestinal health status of pigs at different growth stages.
Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
Authors: Fu Junrui , Wang Junhan , Huang Ximei , Guan Boyang , Feng Qili , Deng Huimin
Published: September 2023

Introduction

Spodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.

Methods

Here, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction.

Results

Proteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction.

Discussion

Our findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.
Spatial diversity of the skin bacteriome
Authors: Pérez-Losada Marcos , Crandall Keith A.
Published: September 2023

The bacterial communities of the human skin impact its physiology and homeostasis, hence elucidating the composition and structure of the healthy skin bacteriome is paramount to understand how bacterial imbalance (i.e., dysbiosis) may lead to disease. To obtain an integrated view of the spatial diversity of the skin bacteriome, we surveyed from 2019 to 2023 five skin regions (belly button, behind ears, between toes, calves and forearms) with different physiological characteristics (dry, moist and sebaceous) in 129 healthy adults (579 samples – after data cleaning). Estimating bacterial diversity through 16S rRNA metataxonomics, we identified significant (p < 0.0001) differences in the bacterial relative abundance of the four most abundant phyla and 11 genera, alpha- and beta-diversity indices and predicted functional profiles (36 to 400 metabolic pathways) across skin regions and microenvironments. No significant differences, however, were observed across genders, ages, and ethnicities. As previously suggested, dry skin regions (forearms and calves) were more even, richer, and functionally distinct than sebaceous (behind ears) and moist (belly button and between toes) regions. Within skin regions, bacterial alpha- and beta-diversity also varied significantly for some of the years compared, suggesting that skin bacterial stability may be region and subject dependent. Our results, hence, confirm that the skin bacteriome varies systematically across skin regions and microenvironments and provides new insights into the internal and external factors driving bacterial diversity.
A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells
Authors: Shao Yunting , Wang Peng , Zheng Yunji , Cui Hongtu , Lou Zhangrong , Li Shanhu , Huang Fang , Wu Chengjun
Published: September 2023

Objective

In past decades, the role of high-risk HPV (HR-HPV) infection in cancer pathogenesis has been extensively studied. The viral E7 protein expressed in pre-malignant cells has been identified as an ideal target for immunological intervention. However, the cultivation of HPV in vitro remains a significant challenge, as well as the lack of methods for expressing the HPV E7 protein and generating replication-competent recombinant viral particles, which posed a major obstacle to further exploration of the function and carcinogenic mechanisms of the E7 oncoprotein. Therefore, it is imperative to investigate novel methodologies to construct replication-competent recombinant viral particles that express the HPV E7 protein to facilitate the study of its function.

Methods

We initiated the construction of recombinant viral particles by utilizing the ccdB-Kan forward/reverse screening system in conjunction with the Red/ExoCET recombinant system. We followed the infection of C33A cells with the obtained recombinant virus to enable the continuous expression of HPV16 E7. Afterwards, the total RNA was extracted and performed transcriptome sequencing using RNA-Seq technology to identify differentially expressed genes associated with HPV-induced oncogenicity.

Results

We successfully established replicative recombinant viral particles expressing HPV16 E7 stably and continuously. The C33A cells were infected with recombinant viral particles to achieve overexpression of the E7 protein. Subsequently, RNA-Seq analysis was conducted to assess the changes in host cell gene expression. The results revealed an upregulation of the CD36 gene, which is associated with the HPV-induced oncogenic pathways, including PI3K-Akt and p53 signaling pathway. qRT-PCR analysis further identified that the upregulation of the CD36 gene due to the expression of HPV16 E7.

Conclusion

The successful expression of HPV16 E7 in cells demonstrates that the replicated recombinant virus retains the replication and infection abilities of Ad4, while also upregulating the CD36 gene involved in the PI3K-Akt signaling and p53 pathways, thereby promoting cell proliferation. The outcome of this study provides a novel perspective and serves as a solid foundation for further exploration of HPV-related carcinogenesis and the development of replicative HPV recombinant vaccines capable of inducing protective immunity against HPV.
Health factors associated with cognitive frailty in older adults living in the community
Authors: Corral-Pérez Juan , Casals Cristina , Ávila-Cabeza-de-Vaca Laura , González-Mariscal Andrea , Martínez-Zaragoza Ildefonsa , Villa-Estrada Francisca , Reina-Campos Remedios , Vázquez-Sánchez María Á.
Published: September 2023

Introduction

This study aims to investigate the health factors associated with cognitive frailty in frail and pre-frail older adults living in the community.

Methods

A total of 233 older adults meeting Fried’s criteria for pre-frailty or frailty were included. Cognitive status was evaluated using the Short Portable Mental Status Questionnaire. Health factors encompassed nutritional status (evaluated using the Mini Nutritional Assessment tool, body mass index, and waist, arm, and leg circumferences), physical function (assessed with the Short Physical Performance Battery), quality of life (measured with the total index of the EuroQoL 5-Dimension 5-Level questionnaire - EQoL-Index -, and the Visual-Analogue Scale - QoL-VAS - for today’s health state), as well as sleep, physical activity, and inactivity estimated through wrist-worn accelerometers. Multivariable logistic regression analyses were conducted to identify potential predictors of cognitive frailty, considering age as a confounding factor.

Results

Cognitive frail participants exhibited advanced age, heightened self-reported exhaustion, diminished overall physical performance, reduced leg perimeter, decreased engagement in moderate-to-vigorous physical activity, and higher levels of inactivity (all p<0.05). However, after adjusting for age, only QoL-VAS emerged as a cognitive frailty risk factor (Odds ratio: 1.024), while the EQoL-Index, calf perimeter, and levels of moderate-to-vigorous physical activity were identified as protective factors (Odds ratios: 0.025, 0.929, and 0.973, respectively).

Discussion

This study highlights the complex relationship between non-modifiable factors such as age, and modifiable factors including quality of life, nutritional status, and physical activity in the development of cognitive frailty among older adults with a frailty phenotype living in the community.
Limited connectedness of spontaneous speech may be a marker of dementia due to Alzheimer’s disease
Authors: Botezatu Mona Roxana , Miller Erika , Kiselica Andrew M.
Published: September 2023

The study evaluated the connectedness of spontaneous speech production in individuals with dementia as a potential predictor of dementia severity. Data were derived from the baseline sample of 143 individuals with dementia in the English Pitt corpus. Dementia severity was assessed via the Mini Mental Status Exam, the Mattis Dementia Rating Scale, and the Blessed Dementia Scale. Language abilities were evaluated using verbal fluency and picture description tasks. Graph analysis was carried out for the picture description task using the computational tool SpeechGraphs to calculate connectedness. Results demonstrated that higher educational attainment, higher verbal fluency and strongly-connected spontaneous speech were associated with better cognitive function. Results suggest that automated language processing approaches, such as graph structure analysis, may provide a faster and ecologically valid method of detecting dementia symptoms.
Fine periodic nanostructure formation on stainless steel and gallium arsenide with few-cycle 7-fs laser pulses
Authors: Ishihara Akihiro , Miyaji Godai
Published: September 2023

We report the fine periodic nanostructure formation process on metal and semiconductor surfaces in air with few-cycle 7-fs laser pulses and its physical mechanism. Using appropriate peak power densities and scanning speeds for the laser pulses, nanostructures could be formed on stainless steel and gallium arsenide (GaAs) with periods of 60–110 nm and 130–165 nm, respectively, which are 1/5–1/4 of the period of nanostructures formed with 100-fs laser pulses. The periodicity can be explained as arising from the excitation of short-range propagating surface plasmon polaritons, and the observed periods are in good agreement with the model calculation results.
Parsing the contributions of negative affect vs. aversive motivation to cognitive control: an experimental investigation
Authors: Yang Qian , Si ShuangQing , Pourtois Gilles
Published: September 2023

Introduction

Punishment is a powerful drive that fosters aversive motivation and increases negative affect. Previous studies have reported that this drive has the propensity to improve cognitive control, as shown by improved conflict processing when it is used. However, whether aversive motivation per se or negative affect eventually drives this change remains unclear because in previous work, the specific contribution of these two components could not be isolated.

Methods

To address this question, we conducted two experiments where we administered the confound minimized Stroop task to a large group of participants each time (N = 50 and N = 47 for Experiment 1 and 2, respectively) and manipulated punishment and feedback contingency using a factorial design. These two experiments were similar except that in the second one, we also measured awareness of feedback contingency at the subjective level. We reasoned that cognitive control would improve the most when punishment would be used, and the contingency between this motivational drive and performance would be reinforced, selectively.

Results

Both experiments consistently showed that negative affect increased at the subjective level when punishment was used and the feedback was contingent on task performance, with these two effects being additive. In Experiment 1, we found that when the feedback was contingent on task performance and punishment was activated, conflict processing did not improve. In Experiment 2, we found that conflict processing improved when punishment was contingent on task performance, and participants were aware of this contingency.

Discussion

These results suggest that aversive motivation can improve conflict processing when participants are aware of the link created between punishment and performance.
Percutaneous electrical nerve field stimulation compared to standard medical therapy in adolescents with functional abdominal pain disorders
Authors: Santucci Neha R. , Sahay Rashmi , El-Chammas Khalil I. , Graham Kahleb , Wheatley Mikaela , Vandenbrink Madeleine , Hardy Jennifer , Fei Lin
Published: September 2023

Introduction

Standard medical therapy (SMT) in children with functional abdominal pain disorders (FAPD) includes cyproheptadine and amitriptyline. While percutaneous electrical nerve field stimulation (PENFS) has shown benefit, no study has compared outcomes of PENFS to SMT. We aimed to examine changes in abdominal pain, nausea and disability before and after treatment and compare outcomes between treatments.

Methods

The records of FAPD patients ages 11–21 years, treated with 4 weeks of PENFS, cyproheptadine or amitriptyline were reviewed. Outcomes were evaluated using validated questionnaires [Abdominal Pain Index (API), Nausea Severity Scale (NSS), and the Functional Disability Inventory (FDI)] at baseline and follow-up within 3 months (FU).

Result

Of 101 patients, 48% received PENFS, 31% cyproheptadine and 21% received amitriptyline. Median ages were 17 (15–19), 16 (15–18) and 15 (11–16) years respectively and the majority were females (75%, 90% and 52% respectively). In the PENFS group, API (p = 0.001), NSS (p = 0.059) and FDI (p = 0.048) were significantly lower at FU. API (p = 0.034) but not NSS and FDI (p > 0.05) decreased significantly at FU in the amitriptyline group. API, NSS and FDI did not change significantly with cyproheptadine at FU (p > 0.05). FU API scores were lower in PENFS vs. cyproheptadine (p = 0.04) but not vs. amitriptyline (p = 0.64). The FDI scores were significantly lower in the amitriptyline vs. cyproheptadine group (p = 0.03).

Conclusion

Therapy with PENFS showed improvements in abdominal pain, nausea and disability while amitriptyline showed improvements in abdominal pain within 3 months of treatment. PENFS was more effective than cyproheptadine in improving abdominal pain. Amitriptyline improved disability scores more than cyproheptadine and showed promise for treatment. PENFS may be a good non-pharmacologic alternative for FAPD.
Inhibitory mechanism of vortioxetine on CYP450 enzymes in human and rat liver microsomes
Authors: Zhan Yunyun , Wang Anzhou , Yu Yige , Chen Jie , Xu Xinhao , Nie Jingjing , Lin Jingjing
Published: September 2023

Vortioxetine is a novel anti-major depression disorder drug with a high safety profile compared with other similar drugs. However, little research has been done on drug-drug interactions (DDI) about vortioxetine. In this paper, the inhibitory effect of vortioxetine on cytochrome P450 (CYP450) and the type of inhibitory mechanism were investigated in human and rat liver microsomes. We set up an in vitro incubation system of 200 μL to measure the metabolism of probe substrates at the present of vortioxetine at 37°C. The concentrations of the metabolites of probe substrates were all measured by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method. It was found no time-dependent inhibition (TDI) of vortioxetine through determination of half-maximal inhibitory concentration (IC50) shift values. The enzymes and metabolites involved in this experiment in human and rats were as follows: CYP3A4/CYP3A (midazolam); CYP2B6/CYP2B (bupropion); CYP2D6/CYP2D (dextromethorphan); CYP2C8/CYP2C-1 (amodiaquine); CYP2C9/CYP2C-2 (losartan); and CYP2C19/CYP2C-3 (mephenytoin). We found that vortioxetine competitively inhibited CYP2C19 and CYP2D6 in human liver microsomes (HLMs) with inhibition constant (Ki) values of 2.17 μM and 9.37 μM, respectively. It was noncompetitive inhibition for CYP3A4 and CYP2C8, and its Ki values were 7.26 μM and 6.96 μM, respectively. For CYP2B6 and CYP2C9, vortioxetine exhibited the mixed inhibition with Ki values were 8.55 μM and 4.17 μM, respectively. In RLMs, the type of vortioxetine inhibition was uncompetitive for CYP3A and CYP2D (Ki = 4.41 and 100.9 μM). The inhibition type was competitive inhibition, including CYP2B and CYP2C-2 (Ki = 2.87 and 0.12 μM). The inhibition types of CYP2C-1 and CYP2C-3 (Ki = 39.91 and 4.23 μM) were mixed inhibition and noncompetitive inhibition, respectively. The study of the above mechanism will provide guidance for the safe clinical use of vortioxetine so that the occurrence of DDI can be avoided.
Protective effects of curcumin against osteoporosis and its molecular mechanisms: a recent review in preclinical trials
Authors: Yang Shenglei , Sun Yuying , Kapilevich Leonid , Zhang Xin’an , Huang Yue
Published: September 2023

Osteoporosis (OP) is one of the most common metabolic skeletal disorders and is commonly seen in the elderly population and postmenopausal women. It is mainly associated with progressive loss of bone mineral density, persistent deterioration of bone microarchitecture, and increased fracture risk. To date, drug therapy is the primary method used to prevent and treat osteoporosis. However, long-term drug therapy inevitably leads to drug resistance and specific side effects. Therefore, researchers are constantly searching for new monomer compounds from natural plants. As a candidate for the treatment of osteoporosis, curcumin (CUR) is a natural phenolic compound with various pharmacological and biological activities, including antioxidant, anti-apoptotic, and anti-inflammatory. This compound has gained research attention for maintaining bone health in various osteoporosis models. We reviewed preclinical and clinical studies of curcumin in preventing and alleviating osteoporosis. These results suggest that if subjected to rigorous pharmacological and clinical trials, naturally-derived curcumin could be used as a complementary and alternative medicine for the treatment of osteoporosis by targeting osteoporosis-related mechanistic pathways. This review summarizes the mechanisms of action and potential therapeutic applications of curcumin in the prevention and mitigation of osteoporosis and provides reference for further research and development of curcumin.
Effectiveness of Shuxuening injection in coronary heart disease: a systematic review and meta-analysis
Authors: Shi Menglong , Sun Tianye , Ji Zhaochen , Ma Yucong , Zhao Min , Yang Fengwen , Zhang Junhua
Published: September 2023

Background: Coronary heart disease (CHD) poses a serious threat to public health, and the current medical management still faces significant challenges. Reliable evidence on the efficacy of Shuxuening injection (SXNI) in CHD is still lacking, even though it is widely used in China.

Purpose: To evaluate the efficacy of SXNI combination therapy in treating CHD.

Methods: A systematic search of eight databases was conducted to identify relevant randomized controlled trials (RCTs) from the inception of each database until June 2023. ROB 2.0, RevMan 5.4, and Stata 15.1 were used for quality evaluation and data analysis. The Grading of Recommendation, Assessment, Development, and Evaluation (GRADE) approach was used to evaluate the quality of evidence.

Results: A total of 3,779 participants from 39 studies were included. The results showed SXNI combination therapy increased the clinical efficacy and decreased the frequency and duration of angina. Furthermore, SXNI combination therapy improved cardiac function of patients by decreasing LVEDD, and increased CI, CO, and LVEF. It also improved blood lipid profiles by increasing HDL, decreasing TC, TG, and LDL. The thrombosis factors of patients were also improved by decreasing FIB, PV, HCT, and HS. Moreover, SXNI combination therapy was superior to the conventional treatment in improving CRP levels, increasing ECG efficacy and BNP. However, due to the limited safety information, reliable safety conclusions could not be drawn. Furthermore, the levels of evidence ranged from very low to moderate due to publication bias and heterogeneity.

Conclusion: SXNI can effectively improve angina symptoms, clinical efficacy, cardiac function, blood lipid indicators, and thrombosis factors of patients with CHD. However, more multi-center and large-sample studies are needed to confirm the conclusions due to the limitations of this study.

Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=399606; Identifier: CRD42023433292.
A 4.8-kW high-efficiency 1050-nm monolithic fiber laser amplifier employing a pump-sharing structure
Authors: Meng Xiangming , Li Fengchang , Yang Baolai , Ye Yun , Chai Junyu , Xi Xiaoming , Wang Peng , Wu Hanshuo , Shi Chen , Zhang Hanwei , Wang Xiaolin , Han Kai
Published: September 2023

The power scaling of ytterbium-doped fiber (YDF) lasers emitting at the wavelength range of 1030 nm–1060 nm has been limited by amplified spontaneous emission (ASE), stimulated Raman scattering (SRS) effect, and transverse mode instability (TMI). These effects pose challenges in achieving a high-output power laser within the range of 1030 nm–1060 nm while maintaining a high signal-to-noise ratio. Based on a counter-pumped fiber laser amplifier utilizing our self-developed ytterbium-doped fiber, we have successfully showcased a 4.8-kW laser output at 1050 nm, accompanied by an 85.3% slope efficiency and nearly diffraction-limited beam quality. By effectively applying ASE and TMI, and controlling the Raman Stokes at ∼17 dB below the primary signal wavelength, we have achieved optimal performance at the maximum power level. This high efficiency has been attained through a pump-sharing structure combined with cost-effective, non-wavelength-stabilized 976-nm laser diodes.
How does shading mitigates the water deficit in young Hymenaea courbaril L. plants?
Authors: Reis Lucas C. , Scalon Silvana P. Q. , Foresti Andressa C. , Dresch Daiane M. , Santos Cleberton C. , Lima Vânia T.
Published: September 2023

Information on tolerance to isolated or combined abiotic stresses is still scarce for tree species, although such stresses are normal in nature. The interactive effect of light availability and water stress has been reported for some native tree species in Brazil but has not been widely investigated. To test the hypothesis that shading can mitigate the stressful effect of water deficit on the photosynthetic and antioxidant metabolism and on the growth of young Hymenaea courbaril L. plants, we evaluated the following two water regimes: a) continuous irrigation – control (I) - 75% field capacity. and b) water deficit (S), characterized by irrigation suspension associated the two following periods of evaluation: P0 - when the photosynthetic rate of plants subjected to irrigation suspension reached values ​​close to zero, with the seedlings being re-irrigated at that moment, and REC - when the photosynthetic rate of the re-irrigated plants of each shading levels reached values ​​similar to those of plants in the control treatment, totaling four treatments: IP0, SP0, IREC, and SREC. The plants of these four treatments were cultivated under the four following shading levels: 0, 30, 50, and 70%, constituting 16 treatments. Intermediate shading of 30 and 50% mitigates the water deficit and accelerates the recovery of H. courbaril. Water deficit associated with cultivation without shading (0%) should not be adopted in the cultivation or transplantation of H. courbaril. After the resumption of irrigation in the REC, the other characteristics presented a recovery under all cultivation conditions. Key message: Intermediate shading of 30 and 50% mitigates the water deficit and accelerates the recovery of H. courbaril.