bluuebunny/crossref_metadata_2025 huggingface dataset:
INTERVENÇÃO NO AUTISMO BASEADA NA MUSICOTERAPIA DE IMPROVISAÇÃO E NO MODELO DIR-FLOORTIME
Authors: Oliveira Stephan Malta , Lampreia Carolina
Published: March 2023

O artigo é resultado de uma pesquisa-ação, na qual foi aplicado um programa de Intervenção clí­nica a uma criança autista. O método foi o estudo de caso. A Intervenção se fundamenta na musicoterapia de improvisação e no modelo DIR floortime, ambos referenciais desenvolvimentistas. Segundo esta perspectiva, o engajamento afetivo é fundamental para o desenvolvimento da comunicação. A musicalidade comunicativa, que fundamenta a musicoterapia de improvisação e se faz presente no engajamento afetivo, apresenta padrões atí­picos no autismo, resultando em padrões atí­picos nas interações sociais e no desenvolvimento da comunicação. A técnica da Intervenção consiste na interação musical improvisada e emocionalmente significativa, seguindo-se o foco de interesse da criança. Foram construí­das categorias para avaliar o desenvolvimento do engajamento afetivo e dos comportamentos comunicativos não-verbais e verbais. Os resultados mostraram que a Intervenção contribuiu para o desenvolvimento do engajamento afetivo e dos comportamentos de antecipação, alternância de turno, imitação e fala referenciada, bem como para o aumento da duração e frequência do contato ocular, sorriso responsivo e vocalizações não-referenciadas. Os comportamentos protodeclarativos, protoimperativos e seguir o apontar não foram desenvolvidos. Novos estudos precisam ser realizados, embora, este tipo de tratamento pareça contribuir para o desenvolvimento das habilidades comunicativas no autismo.
COLORED RESOURCES: FERRAMENTA MUSICOTERAPÊUTICA DE AVALIAÇÃO DO ESTRESSE PSICOLÓGICO
Authors: Chantre Angélica , Herrera Ana , Osorio Ángela , Sarta Ximena , Triviño Sergio
Published: March 2023

A avaliação, nos processos de musicoterapia, tem sido um desafio tanto para a prática clí­nica como para a pesquisa, devido à ausência de ferramentas que possam abordar de forma integral, a riqueza dos dados obtidos pelo musicoterapeuta. Apesar de existirem vários testes psicométricos para o estresse psicológico, torna-se necessário desenvolver novos instrumentos de avaliação musicoterapêutica que permitam integrar conceitos da psicologia com métodos e técnicas próprios da musicoterapia. Esse artigo expõe desenvolvimento de uma ferramenta nova para avaliação em musicoterapia, realizado na disciplina de Psicologia IV, no curso de mestrado em Musicoterapia, na UniversidadeNacional da Colômbia, durante o segundo semestre de 2017.
MUSICOTERAPIA E ADICÇÃO: EFEITOS DA MUSICOTERAPIA NA ADERÊNCIA AO TRATAMENTO DE UM GRUPO DE PACIENTES COM CONSUMO CRÔNICO DE SUBSTÂNCIAS PSICOATIVAS
Authors: Gutiérrez Christiam André Bautista
Published: March 2023

O artigo a seguir sintetiza a implementação de um programa de intervenção de Musicoterapia para usuários com condutas de dependência, realizado entre junho e agosto de 2017, no Centro de Atenção à Toxicodependência Hospital Santa Clara, emBogotá - Colombia. A intervenção teve duração total de 19 sessões, realizadas duas vezes por semana, com a participação voluntária de 10 usuários. O objetivo da pesquisa foi identificar o impacto do trabalho de musicoterapia na adesão ao tratamento dos pacientes diagnosticados com dependência crônica. Também procurou descrever a interação musical e as mudanças no grupo de intervenção durante o processo. Para este fim, a intervenção foi realizada com base na proposta teórica do modelo de terapia analí­tica de Mary Priestley, sustentada na improvisação clí­nica musicoterapêutica. Quanto aos resultados, é possí­vel detalhar que, com a passagem das sessões, os usuários se inclinaram para o modo de interação musical rí­tmica. Deve-se também notar que quatro participantes abandonaram o processo terapêutico. Fato que permitiu identificar variáveis fundamentais para o processo de desenvolvimento da reabilitação de pessoas dependentes de substância psicoactiva, quando o objetivo é o de promover e melhorar a aderência ao tratamento, sem desvincular os objetivos terapêuticos.
MUSICOTERAPIA RECEPTIVA NO TRATAMENTO DA DOR CRÔNICA
Authors: Nemes Maria Cristina , Souza Liliane M. F. Oliveira L.
Published: March 2023

O presente estudo tem como objetivo descrever o trabalho da Musicoterapia com o alí­vio da dor. A pesquisa apresenta cunho descritivo e bibliográfico. Um dos objetivos especí­ficos da pesquisa é também um levantamento bibliográfico sobre dor crônica, música e dor crônica, Musicoterapia e dor crônica. Na literatura são encontradas algumas outras técnicas com a utilização da música e da musicoterapia no alí­vio da dor crônica, são elas: Arrastamento; música pré-escolhida e música preferida; audição de música clássica, técnica em "U" e Entrainment. Essas técnicas trazem alguns benefí­cios fisiológicos como a redução significativa da atividade da banda delta no giro cingulado da dor, diminuição da ansiedade, diminuição de sintomas depressivos e do consumo de agentes ansiolí­ticos.Apresenta ainda o deslocamento do foco da atenção da dor para longe do estí­mulo da dor, e efeitos relevantes na atividade da banda-gama em cortes somatossensoriais primários e secundários. A Musicoterapia relata várias contribuições no alí­vio da dor, dentre elas a técnica Entrainment que demanda o estabelecimento da criação mental da imagem auditiva da dor de forma precisa. O tempo de exposição a esses sons, e a avaliação da intensidade da dor (antes e depois da experiência sonora), foram eficazes, a julgar pelo resultado positivo após a exposição à técnica, melhorando a qualidade de vida, de quem se beneficia, mesmo após um ano.
ENTREVISTA
Authors: Soares Liliam Ribeiro
Published: March 2023

Esta entrevista foi realizada com a Mt. Liliam Ribeiro Soares (CPMT 001/19-MA) em 15 de abril de 2019. Entrevistador: Lázaro Castro Silva Nascimento é Musicoterapeuta(CPMT 346/20-PR) graduado na Universidade Estadual do Paraná (Unespar, 2019) eDoutor em Psicologia Clí­nica e Cultura (UnB). Contato: lazarocsn@live.com
MUSICOTERAPIA COMUNITARIA EN COLOMBIA
Authors: Vasco Andrés Felipe Salgado , GÃ¼iza Diego Alejandro Torres
Published: March 2023

En Colombia, la música ha cumplido una función social y comunitaria muy importante a lo largo de diversos momentos en su historia, particulamente y de forma oficial surge la musicoterapia comunitaria en el año 2007. A partir de este momento se comienzan a compilar y construir acercamientos desde la teorí­a, y sólo hasta el 2012 estos traspasan las fronteras de la teorí­a hacia la praxis en contexto que reúne musicoterapeutas y una comunidad (año 2012), a partir de allí­ se empiezan a diseñar e implementar nuevos trabajos (2014 y 2015). Finalmente, en el año 2016 se logra una consolidación gracias a alianzas de la maestrí­a en musicoterapia de la Universidad Nacional de Colombia con instituciones importantes del territorio y al trabajo de la lí­nea de profundización en musicoterapia comunitaria del mismo programa, logrando consolidar un marco teórico y referencial que da sustento a la práctica desde el contexto de diversas comunidades (victimas del conflicto, excombatientes, infantes y adolescentes en condicion de vulnerabilidad por contextos de violencia y/o desplazamiento forzado, entre otras). Todo esto ha permitido que en la actualidad los musicoterapeutas y estudiantes de la maestrí­a se muestren interesados por trabajar en este paradigma especialmente a favor de objetivos como el fortalecimiento y/o reconstrucción del tejido social, creación de communitas, el empoderamiento y la contrucción de comunidades.
A CIÊNCIA-ARTE DA MUSICOTERAPIA: SOBRE O SEU DESENVOLVIMENTO EM CUBA
Authors: Juan Teresa Fernández de
Published: March 2023

Ao longo do artigo, o principal pano de fundo para o desenvolvimento damusicoterapia em Cuba é explicado como uma visão histórica. Posteriormente, descrevea formação do Grupo (mais tarde Associação) de Musicoterapeutas cubanos, detalhandoos nomes daqueles que semearam uma referência importante nele, começando no séculoancestral. O artigo é ilustrado com fotos que acompanham esta bela obra, com as datasda fundação do grupo inicial até o presente; e os tempos em que Cuba se juntou, comoum grupo de musicoterapia, as várias organizações internacionais e latino-americanas aque pertence. Ele detalha as realizações individuais e de grupo que foram alcançadas,bem como as diferentes linhas de aplicação e pesquisa de seus membros. Alguns dosprofessores musicoterapeuta que têm cooperado com a sua formação e desenvolvimentotambém estão incluí­dos. Dentro do artigo, todas as publicações do grupo foram inseridas,desde a sua formação até os dias atuais, para a entrada de os leitores.
O TEATRO E O DESENHO COMO RECURSOS NA MUSICOTERAPIA: Relato de Experiência
Authors: Nogueira Priscila Lageano , Rosário Verônica Magalhães
Published: March 2023

As artes expressivas possibilitam uma série de benefí­cios a diversos públicos, incluindo pessoas com ou sem deficiência. Tais recursos podem ser empregados na Musicoterapia. Porém, existem poucos materiais sobre esse assunto. Este artigo é um relato de experiência que descreve um processo musicoterapêutico realizado em um projeto de extensão da Escola de Música da Universidade Federal de Minas Gerais (UFMG). O objetivo principal deste estudo é abordar o emprego e a associação de duas artes expressivas (desenho e o teatro), nos atendimentos de Musicoterapia. Através da experiência relatada, foi possí­vel retratar a importância do tema referido e apresentar algumas possibilidades de técnicas e atividades utilizadas, além de descrever um exemplo de quando e como utilizar outras artes expressivas na Musicoterapia. Consequentemente, este trabalho também demonstra a importância da existência de projetos de extensão nas universidades e da experiência agregada pelos estágios curriculares para a formação de alunos e futuros profissionais da saúde, especificamente musicoterapeutas.Palavras-chave: Musicoterapia. Artes Expressivas. Relato de Experiência.
ENTREVISTA
Authors: Chagas Marly , Madalozzo Tiago
Published: March 2023

Entre os meses de março e abril de 2021, Rodrigo Vicente e Rosemyriam Cunha,professores(as) do bacharelado em Musicoterapia da UNESPAR, elaboraram uma série deperguntas com o objetivo de ouvir e conhecer as percepções de um(a) musicoterapeuta ede um(a) educador(a) musical acerca de temas comuns a ambas as áreas. Estruturadas no formato de entrevista, as perguntas foram enviadas por e-mail para convidados(as) muitoespeciais: Marly Chagas1, musicoterapeuta e professora da UFRJ, e Tiago Madalozzo2,educador musical e professor da UNESPAR – Campus Curitiba II. As afinidades eparticularidades de cada resposta transparecem a riqueza de possibilidades de experiênciae vivência no campo da música, em visões renovadas e inspiradoras acerca dos fazeres esaberes da Musicoterapia e da Educação Musical.
Dialética para Responder às Demandas da Época Work in Progresss. Linguagem Poética. Contexto da Ciência e Tecnologia
Authors: Thomaz Didonet , Silveira Luciana Martha
Published: March 2023

Esta investigação consiste em atos de releitura, reflexão e escritura acerca da vida e da visão de mundo construí­da por Walter Benjamin. Isso, com vistas a embasar teoricamente uma hipótese de work in progress por meio da articulação da linguagem poética proveniente do locus da prática artí­stica, no contexto da ciência e da tecnologia.
Un análisis iconográfico de la Estela Ponce, Tiwanaku, Bolivia.
Authors: Torres Constantino Manuel , Torres Donna
Published: March 2023

En este trabajo se presenta un análisis iconográfico detallado de la estela Ponce y su relación con el uso de inhalantes visionarios. Primero se examina la posición y contexto de la escultura dentro del sitio arqueológico. Segundo, los componentes iconográficos se identifican y categorizan. Esta sección incluye discusión de la relación del cuerpo humano con los diferentes temas y motivos grabados en la anatomí­a del monumento. Tercero, se presentan modelos de posible organización de la configuración iconográfica. Cuarto, concluye con comparaciones entre las estelas Ponce y Bennett, y su relación con la parafernalia inhalatoria. La evidencia presentada en este artí­culo sugiere que las experiencias extáticas provocadas por inhalantes y pociones contribuyeron a la formación y desarrollo de la configuración iconográfica Tiwanaku.
Um Memento mori de Henrique Bernadelli?
Authors: Junior Rafael Alves Pinto
Published: March 2023

Resumo – este estudo se propõe a analisar a pintura Os últimos momentos de um Bandeirante executado por Henrique Bernadelli (1857-1936) em 1932 como um ponto final à série temática dedicada à ornamentação do Museu Paulista. A pintura representa a morte de Fernão Dias Paes Leme e pode ser vista como um memento mori devido ao sentido trágico ressignificado da figura do bandeirante.Palavras chave – Pintura brasileira, Bernadelli, bandeirantes.
Camisas azuis, mangas arregaçadas: aspectos não-verbais e simbólicos presentes nas campanhas eleitorais de 2006.
Authors: Bloomfield Tânia Bittencourt
Published: March 2023

O artigo tem o objetivo de possibilitar uma discussão sobre os papéis que determinados elementos visuais e não-verbais, integrantes do discurso polí­tico, cumprem em campanhas eleitorais. Como estes elementos se articulam com as representações sociais e o imaginário do eleitorado, de forma a serem usados como fatores de persuasão na obtenção da atenção e dos votos dos eleitores? A partir de referencial teórico oriundo dos campos da Análise de Discurso, do Marketing Polí­tico e da Cultura Visual, e de fontes imagéticas da mí­dia impressa, televisiva, e da História da Arte, realiza-se um estudo de caso da campanha eleitoral brasileira à Presidência da República e demais cargos eletivos de 2006.
Interações entre natureza e ciência na arte contemporânea
Authors: Fortes Júnior Hugo Fernando Salinas
Published: March 2023

O presente artigo apresenta parcialmente alguns dos resultados obtidos durante a pesquisa de pós-doutorado realizada em 2009 na FAU-USP. A pesquisa investiga as interações entre natureza e ciência na arte contemporânea, procurando identificar as recentes mudanças de paradigmas na relação do homem com a paisagem natural. São apresentadas reflexões sobre artificialidade da percepção humana da natureza e as implicações que isto traz para a arte contemporânea.
AS MÚLTIPLAS FACETAS DAS FOTOGRAFIAS DE TINA MODOTTI: Os diálogos com as artes e lutas mexicanas na década de 1920
Authors: Muzardo Fabiane Tais
Published: March 2023

O presente artigo visa discutir os desdobramentos na produção fotográfica de Tina Modotti, no México, em meados de 1920. Parte-se da ideia de que os diversos contatos de Tina com o povo mexicano, com artistas e intelectuais mexicanos e estrangeiros, fizeram com que sua fotografia fosse constantemente repensada e reelaborada, unindo preocupações éticas e estéticas. Objetiva-se, desse modo, romper com uma análise cronológica de sua produção, que acaba por dividir suas fotografias em fases, e pensar em sua obra como algo com constantes e múltiplas conexões.
IMAGENS MENTAIS ESPONTÂNEAS: Mente, Alma, Corpo e Processos Visionários durante o uso ritual de Ayahuasca
Authors: Mercante Marcelo Simão
Published: March 2023

Pretendo neste artigo fazer uma breve análise do processo de "miração" , ou "imagens mentais espontâneas" , que é o processo imaginativo experienciado durante o uso ritual da ayahuasca, bebida psicoativa utilizada por indí­genas amazônicos e em algumas religiões brasileiras. A imaginação é vista como uma "força" , ou o poder da mente de distorcer imagens, e não de cria-las. A imaginação, longe de ser vista como algo relacionado à ficção ou à irrealidade, é entendida então como a base da realidade, influenciando e sendo influenciada por estados mentais, emocionais, fí­sicos/corporais. Sendo assim, os processos imaginativos podem ser vistos como capazes de modificação de comportamento.
A potential cost savings analysis of a penicillin de-labeling program
Authors: Dong Yilu , Zembles Tracy N. , Nimmer Mark , Brousseau David C. , Vyles David
Published: March 2023

Introduction

Over 95% of patients documented as penicillin allergic can tolerate a penicillin without a reaction. Inaccurate documentation of penicillin allergy leads to more expensive alternative antibiotic prescriptions and an increased incidence of resistant infections.

Objective

To understand the potential drug cost savings of a penicillin de-labeling program to a healthcare system.

Methods

We evaluated patient visits with documented penicillin allergy who presented to the pediatric Emergency Department (PED) and 22 associated primary care clinics. Patients were included if they were discharged home with a non-penicillin antibiotic when the first-line treatment for the diagnosis would have been a penicillin. The potential cost savings were the sum of all visit-level cost differences between the non-penicillin prescription(s) and a counterfactual penicillin prescription. To factor in a 95% successful patient de-labeling rate, we repeatedly sampled 95% from the patients with the eligible visits 10,000 times to produce an estimate of the potential cost savings.

Results

Over the 8-year period, 2,034 visits by 1,537 patients to the PED and 12,349 visits by 6,073 patients to primary care clinics satisfied eligibility criteria. If 95% of the patients could have been successfully de-labeled, it would have generated a cost saving of $618,653 (95% CI $618,617—$618,689) for all the corresponding payers in the system.

Conclusions

Implementing a penicillin de-labeling program across a healthcare system PED and its associated primary care clinics would bring significant cost savings. Healthcare systems should rigorously evaluate optimal methods to de-label patients with reported penicillin allergy.
Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
Authors: Xing Hongguan , Wang Pingping , Yan Xing , Yang Yi , Li Xinliang , Liu Rui , Zhou Zhihua
Published: March 2023

Phytase efficiently hydrolyzes phytate to phosphate; thus, it is widely used to increase phosphorus availability in animal feeds and reduce phosphorus pollution through excretion. Phytase is easily inactivated during feed pelleting at high temperature, and sufficient thermostability of phytase is essential for industrial applications. In this study, directed evolution was performed to enhance phytase thermostability. Variants were initially expressed in Escherichia coli BL21 for screening, then in Pichia pastoris for characterization. Over 19,000 clones were generated from an error-prone Polymerase Chain Reaction (epPCR) library; 5 mutants (G10, D7, E3, F8, and F9) were obtained with approximately 9.6%, 10.6%, 11.5%, 11.6%, and 12.2% higher residual activities than the parent after treatment at 99°C for 60 min. Three of these mutants, D7, E3, and F8, exhibited 79.8%, 73.2%, and 92.6% increases in catalytic efficiency (kcat/Km), respectively. In addition, the specific activities of D7, E3, and F8 were 2.33-, 1.98-, and 2.02-fold higher than parental phytase; they were also higher than the activities of all known thermostable phytases. Sequence analysis revealed that all mutants were substituted at residue 75 and was confirmed that the substitution of cysteine at position 75 was the main contribution to the improvement of thermostability of mutants by saturation mutagenesis, indicating that this amino acid is crucial for the stability and catalytic efficiency of phytase. Docking structure analysis revealed that substitution of the C75 residue allowed the mutants to form additional hydrogen bonds in the active pocket, thereby facilitating binding to the substrate. In addition, we confirmed that the intrinsic C77-C108 disulfide bond in E. coli phytase is detrimental to its stability.
Understanding the position of urban spatial configuration on the feeling of insecurity from crime in public spaces
Authors: Widya Putra Dimas , Salim Wilmar A. , Indradjati Petrus Natalivan , Prilandita Niken
Published: March 2023

Introduction: Many studies have discussed crime and the feeling of insecurity in the public space. These studies produce diverse findings from various variables and different objects. However, the urban spatial configuration is still left unexplored in the study of insecurity from crime in the public space. The purpose of this study is to discuss the position of urban spatial configuration elements on the feeling of insecurity from crime in the public space evidenced by international literature.

Methods: This study uses a systematic review method by evaluating studies published between 1970 and 2021.

Results and Discussion: The results of this study state that studies on crime and feeling of insecurity in public spaces in urban planning and urban design context mainly evaluated the design features (crime prevention through environmental design) and Socio-demographics at micro–meso scale in the built environment. In contrast, urban spatial configuration variable or macro scale still underexplored. Studies have shown that issues will occur if policy-making and planning in urban areas ignore individual perceptions of feeling insecure regarding crime at macro scale. The findings of this study become a research gap for further studies. This study suggests that more researchers should evaluate the feelings of insecurity from crime in public spaces on a macro scale, namely the urban spatial configuration dimension, including city size, development type, the distribution pattern of population and job, degree of clustering, and landscape connectivity.
Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing
Authors: Torres-Flores Ulises , Díaz-Espinosa Fernanda , López-Santaella Tayde , Rebollar-Vega Rosa , Vázquez-Jiménez Aarón , Taylor Ian J. , Ortiz-Hernández Rosario , Echeverría Olga M. , Vázquez-Nin Gerardo H. , Gutierrez-Ruiz María Concepción , De la Rosa-Velázquez Inti Alberto , Resendis-Antonio Osbaldo , Hernández-Hernandez Abrahan
Published: March 2023

CTCF is an architectonic protein that organizes the genome inside the nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, defects produced by its depletion throughout spermatogenesis have not been fully characterized. In this work, we performed single cell RNA sequencing in spermatogenic cells with and without CTCF. We uncovered defects in transcriptional programs that explain the severity of the damage in the produced sperm. In the early stages of spermatogenesis, transcriptional alterations are mild. As germ cells go through the specialization stage or spermiogenesis, transcriptional profiles become more altered. We found morphology defects in spermatids that support the alterations in their transcriptional profiles. Altogether, our study sheds light on the contribution of CTCF to the phenotype of male gametes and provides a fundamental description of its role at different stages of spermiogenesis.
Regulatory mechanism and research progress of ferroptosis in obstetrical and gynecological diseases
Authors: Wang Xinyue , Wei Yanchen , Wei Fangyi , Kuang Haibin
Published: March 2023

Ferroptosis is a novel type of regulated cell death driven by iron-dependent lipid peroxidation, which is distinguished from traditional types of programmed cell death, such as apoptosis, proptosis and necrosis et al. Impaired iron homeostasis, lipid peroxidation and antioxidants depletion are three hallmarks of ferroptosis. Over the past years, emerging studies support the notion that ferroptosis might be involved in the pathology of obstetrical and gynecological diseases, including preeclampsia (PE), endometriosis (EMs) and polycystic ovarian syndrome (PCOS). In the PE condition, the high sensitivity of trophoblasts towards ferroptosis has been found to potentially link to inflammation, suboptimal vascular remodeling and aberrant hemodynamics, which are three prominent pathophysiological features of PE. As for EMs, compromised ferroptosis of endometrial cells was associated with the formation ectopic lesions, whereas in the nearby lesions, the presence of ferroptosis was suggested to promote the progression of EMs, contributing to the relative clinical manifestations. Ferroptosis has been implicated a crucial role in the initiation of ovarian follicular atresia, which might help to manage ovulation in PCOS patients. Taken together, this review explored the basis of ferroptosis mechanisms and comprehensively summarized the latest discovery of roles of ferroptosis on PE, EMs and PCOS, gaining a deeper insight into the pathogenesis of these obstetrical and gynecological diseases and investigation of novel therapeutic interventions.
Mussel-inspired nanoparticle composite hydrogels for hemostasis and wound healing
Authors: Cui Guihua , Guo Xiaoyu , Su Ping , Zhang Tianshuo , Guan Jiao , Wang Chungang
Published: March 2023

Uncontrolled hemorrhage caused by trauma can easily lead to death. Efficient and safe hemostatic materials are an urgent and increasing need for hemostatic research. Following a trauma, wound healing is induced by various cellular mechanisms and proteins. Hemostatic biomaterials that can not only halt bleeding quickly but also provide an environment to promote wound healing have been the focus of research in recent years. Mussel-inspired nanoparticle composite hydrogels have been propelling the development of hemostatic materials owing to their unique advantages in adhesion, hemostasis, and bacteriostasis. This review summarizes the hemostatic and antimicrobial fundamentals of polydopamine (PDA)-based nanomaterials and emphasizes current developments in hemorrhage-related PDA nanomaterials. Moreover, it briefly discusses safety concerns and clinical application problems with PDA hemostatic nanomaterials.
Incidence and predictors of left ventricular function change following ST-segment elevation myocardial infarction
Authors: Liu Chuanfen , Guo Meng , Cui Yuxia , Wu Manyan , Chen Hong
Published: March 2023

Aim

The purpose of the study was to assess the incidence and predictors of left ventricular function change in patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary PCI.

Methods

312 patients with STEMI who received primary percutaneous coronary intervention (PCI) between January 2015 and December 2016 were consecutively enrolled in this study. Multiple logistic regression analysis was used to evaluate independent predictors of left ventricular ejection fraction (LVEF) improvement after long-term follow-up.

Results

We finally analyzed the LVEF change in 186 patients from baseline to follow-up. The mean age was 61.3 ± 12.5 years, with 78.5% being male. The median duration of follow-up after STEMI was 1,021 (389–1,947) days. 54.3% had a decrease in LVEF and 45.7% experienced an improvement in LV function after primary PCI through long-term follow-up. Logistic regression analysis showed lower peak troponin I, non-anterior STEMI, lower baseline LVEF, and no previous myocardial infarction history were independently associated with LVEF improvement.

Conclusion

54.3% of patients with STEMI undergoing primary PCI had a decrease in LVEF during long-term follow-up. LVEF recovery can be predicted by baseline characteristics.
Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor
Authors: Sylvester Christopher B. , Amirkhosravi Farshad , Bortoletto Angelina S. , West William J. , Connell Jennifer P. , Grande-Allen K. Jane
Published: March 2023

Calcific aortic valve disease (CAVD), a fibrocalcific thickening of the aortic valve leaflets causing obstruction of the left ventricular outflow tract, affects nearly 10 million people worldwide. For those who reach end-stage CAVD, the only treatment is highly invasive valve replacement. The development of pharmaceutical treatments that can slow or reverse the progression in those affected by CAVD would greatly advance the treatment of this disease. The principal cell type responsible for the fibrocalcific thickening of the valve leaflets in CAVD is valvular interstitial cells (VICs). The cellular processes mediating this calcification are complex, but calcium second messenger signaling, regulated in part by the ryanodine receptor (RyR), has been shown to play a role in a number of other fibrocalcific diseases. We sought to determine if the blockade of calcium signaling in VICs could ameliorate calcification in an in vitro model. We previously found that VICs express RyR isotype 3 and that its modulation could prevent VIC calcific nodule formation in vitro. We sought to expand upon these results by further investigating the effects of calcium signaling blockade on VIC gene expression and behavior using dantrolene, an FDA-approved pan-RyR inhibitor. We found that dantrolene also prevented calcific nodule formation in VICs due to cholesterol-derived lysophosphatidylcholine (LPC). This protective effect corresponded with decreases in intracellular calcium flux, apoptosis, and ACTA2 expression but not reactive oxygen species formation caused by LPC. Interestingly, dantrolene increased the expression of the regulator genes RUNX2 and SOX9, indicating complex gene regulation changes. Further investigation via RNA sequencing revealed that dantrolene induced several cytoprotective genes that are likely also responsible for its attenuation of LPC-induced calcification. These results suggest that RyR3 is a viable therapeutic target for the treatment of CAVD. Further studies of the effects of RyR3 inhibition on CAVD are warranted.
Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and atherosclerosis
Authors: Mo Shuangyang , Wang Yingwei , Yuan Xin , Wu Wenhong , Zhao Huaying , Wei Haixiao , Qin Haiyan , Jiang Haixing , Qin Shanyu
Published: March 2023

Background

Atherosclerosis (AS) is one of the leading causes of the cardio-cerebral vascular incident. The constantly emerging evidence indicates a close association between nonalcoholic fatty liver disease (NAFLD) and AS. However, the exact molecular mechanisms underlying the correlation between these two diseases remain unclear. This study proposed exploring the common signature genes, pathways, and immune cells among AS and NAFLD.

Methods

The common differentially expressed genes (co-DEGs) with a consistent trend were identified via bioinformatic analyses of the Gene Expression Omnibus (GEO) datasets GSE28829 and GSE49541, respectively. Further, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. We utilized machine learning algorithms of lasso and random forest (RF) to identify the common signature genes. Then the diagnostic nomogram models and receiver operator characteristic curve (ROC) analyses were constructed and validated with external verification datasets. The gene interaction network was established via the GeneMANIA database. Additionally, gene set enrichment analysis (GSEA), gene set variation analysis (GSVA), and immune infiltration analysis were performed to explore the co-regulated pathways and immune cells.

Results

A total of 11 co-DEGs were identified. GO and KEGG analyses revealed that co-DEGs were mainly enriched in lipid catabolic process, calcium ion transport, and regulation of cytokine. Moreover, three common signature genes (PLCXD3, CCL19, and PKD2) were defined. Based on these genes, we constructed the efficiently predictable diagnostic models for advanced AS and NAFLD with the nomograms, evaluated with the ROC curves (AUC = 0.995 for advanced AS, 95% CI 0.971–1.0; AUC = 0.973 for advanced NAFLD, 95% CI 0.938–0.998). In addition, the AUC of the verification datasets had a similar trend. The NOD-like receptors (NLRs) signaling pathway might be the most crucial co-regulated pathway, and activated CD4 T cells and central memory CD4 T cells were significantly excessive infiltration in advanced NAFLD and AS.

Conclusion

We identified three common signature genes (PLCXD3, CCL19, and PKD2), co-regulated pathways, and shared immune features of NAFLD and AS, which might provide novel insights into the molecular mechanism of NAFLD complicated with AS.
Transcriptional regulation of macrophages in heart failure
Authors: Wang Keyan , Sun Xiaoqian , Sun Ying , Jiao Boyang , Yao Junkai , Hu Yueyao , Deng Qiong , Dong Jianteng , Wang Wei , Wang Yong , Li Chun
Published: March 2023

Adverse cardiac remodeling after acute myocardial infarction is the most important pathological mechanism of heart failure and remains a major problem in clinical practice. Cardiac macrophages, derived from tissue resident macrophages and circulating monocyte, undergo significant phenotypic and functional changes following cardiac injury and play crucial roles in inflammatory response and tissue repair response. Currently, numerous studies indicate that epigenetic regulatory factors and transcription factors can regulate the transcription of inflammatory and reparative genes and timely conversion of inflammatory macrophages into reparative macrophages and then alleviate cardiac remodeling. Accordingly, targeting transcriptional regulation of macrophages may be a promising option for heart failure treatment. In this review, we not only summarize the origin and function of cardiac macrophages, but more importantly, describe the transcriptional regulation of macrophages in heart failure, aiming to provide a potential therapeutic target for heart failure.
Unsupervised domain adaptation methods for cross-species transfer of regulatory code signals
Authors: Latyshev Pavel , Pavlov Fedor , Herbert Alan , Poptsova Maria
Published: March 2023

Due to advances in NGS technologies whole-genome maps of various functional genomic elements were generated for a dozen of species, however experiments are still expensive and are not available for many species of interest. Deep learning methods became the state-of-the-art computational methods to analyze the available data, but the focus is often only on the species studied. Here we take advantage of the progresses in Transfer Learning in the area of Unsupervised Domain Adaption (UDA) and tested nine UDA methods for prediction of regulatory code signals for genomes of other species. We tested each deep learning implementation by training the model on experimental data from one species, then refined the model using the genome sequence of the target species for which we wanted to make predictions. Among nine tested domain adaptation architectures non-adversarial methods Minimum Class Confusion (MCC) and Deep Adaptation Network (DAN) significantly outperformed others. Conditional Domain Adversarial Network (CDAN) appeared as the third best architecture. Here we provide an empirical assessment of each approach using real world data. The different approaches were tested on ChIP-seq data for transcription factor binding sites and histone marks on human and mouse genomes, but is generalizable to any cross-species transfer of interest. We tested the efficiency of each method using species where experimental data was available for both. The results allows us to assess how well each implementation will work for species for which only limited experimental data is available and will inform the design of future experiments in these understudied organisms. Overall, our results proved the validity of UDA methods for generation of missing experimental data for histone marks and transcription factor binding sites in various genomes and highlights how robust the various approaches are to data that is incomplete, noisy and susceptible to analytic bias.
Automated observation of physical snowpack properties in Ny-Ålesund
Authors: Scoto Federico , Pappaccogli Gianluca , Mazzola Mauro , Donateo Antonio , Salzano Roberto , Monzali Matteo , de Blasi Fabrizio , Larose Catherine , Gallet Jean-Charles , Decesari Stefano , Spolaor Andrea
Published: March 2023

The snow season in the Svalbard archipelago generally lasts 6–10 months a year and significantly impacts the regional climate, glaciers mass balance, permafrost thermal regime and ecology. Due to the lack of long-term continuous snowpack physical data, it is still challenging for the numerical snow physics models to simulate multi-layer snowpack evolution, especially for remote Arctic areas. To fill this gap, in November 2020, an automated nivometric station (ANS) was installed ∼1 km Southwest from the settlement of Ny-Ålesund (Spitzbergen, Svalbard), in a flat area over the lowland tundra. It automatically provides continuous snow data, including NIR images of the fractional snow-cover area (fSCA), snow depth (SD), internal snow temperature and liquid water content (LWC) profiles at different depths with a 10 min time resolution. Here we present the first-year record of automatic snow preliminary measurements collected between November 2020 and July 2021 together with weekly manual observations for comparison. The snow season at the ANS site lasted for 225 days with an annual net accumulation of 117 cm (392 mm of water equivalent). The LWC in the snowpack was generally low (<4%) during wintertime, nevertheless, we observed three snow-melting events between November and February 2021 and one in June 2021, connected with positive temperature and rain on snow events (ROS). In view of the foreseen future developments, the ANS is the first automated, comprehensive snowpack monitoring system in Ny-Ålesund measuring key essential climate variables needed to understand the seasonal evolution of the snow cover on land.
Students’ basic psychological needs in blended teacher learning groups
Authors: De Vocht Ann , Vrieling-Teunter Emmy , Sins Patrick , Vermeulen Marjan
Published: March 2023

Introduction

Student teachers (henceforth: students) in higher education often experience feelings of emotional loneliness that negatively impact upon their well-being and motivation to learn. Consequently, the importance of social learning for students has gained increased prominence, with Teacher Learning Groups (TLGs), that is, social configurations in which students, in-service teachers, and teacher educators, sometimes supplemented by researchers and/or experts, collaboratively learn through social interactions, being introduced in teacher training institutes. Ordinarily, TLGs organized their meetings face-to-face; however, due to COVID-19 measures, they had to rapidly transition to blended meetings, which in turn impacted upon students’ basic psychological needs.

Methods

In the present study, a convergent parallel mixed-methods design was utilized. The variables Social Configurations (Practice integration, Long-term orientation and goals; Shared identity and equal relationships) and Basic Psychological Needs (Competence, Autonomy, Relatedness) were assessed through the use of qualitative interviews and by administering two online quantitative surveys: the “Dimensions of Social Learning Questionnaire” and the “Basic Psychological Need Satisfaction and Frustration Scale”. Seventy students completed the questionnaires, while 14 students were interviewed. The students were recruited from four teacher training institutes.

Results

The analyses reveal that the more students perceive Shared identity and equal relationships in blended TLGs, the greater the fulfillment of Basic Psychological Needs they experience. Moreover, the more students experience the fulfillment of the need for Competence, the more students perceive TLGs’ Social Configurations.

Discussion

Based on the findings, we conclude that, although in-depth learning is more challenging during distance learning, blended TLGs are valuable for students’ Basic Psychological Needs during unpredictable times.
Mapping biodiversity and cultural values complemented with understanding of social dynamics provides effective means for addressing opportunities for nature conservation in a cultural landscape
Authors: Morariu Simona-Diana , Macicasan Vlad , Malos Cristian Valeriu , Hartel Tibor
Published: March 2023

The ecosystem services framework can usefully complement biodiversity assessments in developing socially robust nature conservation strategies in protected areas and beyond. However, there is still little research, especially in Eastern Europe, which links biodiversity assessment and nature related values to the deeper social-economic dynamics and aspirations existing in the local communities. Here we address this knowledge gap with a study case from Romania. We used data from a thorough biodiversity survey to map the protected species and habitats in a Natura 2000 area. Then we used participatory mapping with key local community representatives to understand the type of values linking the local community with the protected area, distinguishing between the past (1960’s-1989 period) and present. We identified that socio-economic and cultural values were strong and synergistically manifested in the past but not in the present. A dramatic abandonment of land use practices was reported. The current distribution of protected species and habitats does not overlap with the farmed areas in the past and present. Interviewees report that the local community went through dramatic changes from the past to present: increased individualism weakened or lost local rules, diversifying individual aspirations, low level of collective actions and weak alignment between knowledge and aspirations and the protected area. New socio-cultural values are represented by educational activities, however, with a lack of genuine local community engagement. Key directions identified by participants for the future of the site were nature friendly activities such as tourism, biking trails, forestry, and the renewal of viticulture.
How urban development affects green development efficiency in China: Taking the city cluster of Yangtze river economic belt as an example
Authors: Huang Jing , Shangguan Ziheng , Gui Tiantian , Liu Jianping
Published: March 2023

Introduction: In order to change the long-term economic development model which is at the expense of the environment, China proposed a series of new energy development and environmental intervention policies. However, there is no evidence to support the impact of urban development on the green development efficiency (GDE) under China’s macroeconomic policies at present. In order to answer this research question and help the government to improve the GDE, this study takes the Yangtze River Economic Belt (YREB) city cluster (41 cities) in China as an example to conduct an empirical study.

Methods: We first measure the GDE of these cities by Slack Based Model (SBM). Then, the Tobit model is used to measure the impact of urban development elements, namely population urbanization, land urbanization, and urban industrial development, on GDE.

Results and Discussion: The analysis of the data leads to the following conclusions and suggestions: 1) The GDE of the city cluster in the YREB has generally been improved driven by policies. But compared to policy interventions, the level of science, technology and management are the primary drivers of long-term improvement in GDE. 2) In order to avoid the economic development and environmental capacity failure to afford the increase in energy consumption and pollution discharge caused by population urbanization and land urbanization, local governments need to promote energy transformation, increase environmental protection efforts and attract top-tier talent. 3) The improvement of GDE by industrial upgrading is a dynamic and slow process, and blindly pushing industrial restructuring will lead to a decline in GDE. Therefore, the Chinese government needs to provide an ecologically suitable environment for local industrial development, which needs to avoid “pulling out the seedlings” and “one-size-fits-all” approach.
Integration analysis based on fatty acid metabolism robustly predicts prognosis, dissecting immunity microenvironment and aiding immunotherapy for soft tissue sarcoma
Authors: Liu Binfeng , He Shasha , Li Chenbei , Feng Chengyao , Wang Hua , Zhang Haixia , Tu Chao , Li Zhihong
Published: March 2023

Background: Soft tissue sarcoma (STS) is a highly malignant tumor with a dismal prognosis. Presently, the dysregulation of fatty acid metabolism has received increasing attention in tumor research, but fewer reports are relevant to STS.

Methods: Based on fatty acid metabolism-related genes (FRGs), a novel risk score for STS was developed utilizing univariate analysis and least absolute shrinkage selection operator (LASSO) Cox regression analyses in the STS cohort, which were further validated using the external validation cohort from other databases. Furthermore, independent prognostic analysis, C-index, ROC curves, and nomogram were carried out to investigate the predictive performance of fatty acid-related risk scores. We also analysed the differences in enrichment pathways, the immune microenvironment, gene mutations, and immunotherapy response between the two distinct fatty acid score groups. Moreover, the real-time quantitative polymerase chain reaction (RT-qPCR) was used to further verify the expression of FRGs in STS.

Results: A total of 153 FRGs were retrieved in our study. Next, a novel fatty acid metabolism-related risk score (FAS) was constructed based on 18 FRGs. The predictive performance of FAS was also verified in external cohorts. In addition, the independent analysis, C-index, ROC curve, and nomograph also revealed that FAS could serve as an independent prognostic factor for the STS patients. Meanwhile, our results demonstrated that the STS cohort in two distinct FAS groups had different copy number variations, immune cell infiltration, and immunotherapy responses. Finally, the in vitro validation results demonstrated that several FRGs included in the FAS exhibited abnormal expression in STS.

Conclusion: Altogether, our work comprehensively and systematically clarifies fatty acid metabolism’s potential roles and clinical significance in STS. The novel individualized score based on fatty acid metabolism may be provided as a potential marker and treatment strategy in STS.
Choice of a family planning outlet in urban areas: The role of distance and quality of services in Kenya and Uganda
Authors: Winston Jennifer , Calhoun Lisa M. , Guilkey David , Macharia Peter M. , Speizer Ilene S.
Published: March 2023

Introduction

Quality of care and physical access to health facilities affect facility choice for family planning (FP). These factors may disproportionately impact young contraceptive users. Understanding which components of service quality drive facility choice among contraceptive users of all ages can inform strategies to strengthen FP programming for all potential users of FP.

Methods

This study uses data from Population Services International's Consumer's Market for Family Planning (CM4FP) project, to examine drivers of facility choice among female FP users. The data collected from female contraceptive users, the outlet where they obtained their contraceptive method, and the complete set of alternative outlets in select urban areas of Kenya and Uganda were used. We use a mixed logit model, with inverse probability weights to correct for selection into categories of nonuse and missing facility data. We consider results separately for youth (18–24) and women aged 25–49 in both countries.

Results

We find that in both countries and across age groups, users were willing to travel further to public outlets and to outlets offering more methods. Other outlet attributes, including signage, pharmacy, stockouts, and provider training, were important to women in certain age groups or country.

Discussion

These results shed light on what components of service quality drive outlet choice among young and older users and can inform strategies to strengthen FP programming for all potential users of FP in urban settings.