bluuebunny/crossref_metadata_2025 huggingface dataset:
Drag on circular cylinders with porous outer layers in turbulent cross-flow
Authors: Cohen Alexandre , Blais Donovan , Mydlarski Laurent , Baliga B Rabi
Published: March 2024

The effects of free-stream turbulence intensity and porosity on the drag on cylinders with porous outer layers in cross-flow was investigated experimentally. This work is motivated by the need to better model spotting—a forest fire propagation mechanism in which burning branches and other debris (termed firebrands) are transported away from the main fire by the prevailing wind and ignite new fires. Multiple levels of background turbulence were studied by using no grid, passive grids, and an active grid to generate turbulence intensities of 0.4%, 1.7%, 2.7% and 12.4%. The porous char-layer on the outer surface of firebrands was mimicked by wrapping wire meshes (of 10, 20 and 40 pores per cylinder diameter or PPD) around the cylinders, each to three different layer-thickness fractions (

1

/

16

,

1

/

8

and

1

/

4

) of the cylinder’s outer diameter. The drag on one smooth cylinder and nine cylinders with porous outer layers was measured for Reynolds numbers in the range 7000–17 000, at the aforementioned four turbulence intensities. The results showed that (i) the free-stream turbulence intensity and PPD of the wire meshes affect the Reynolds number dependence of the drag coefficient; (ii) the drag coefficient increases with free-stream turbulence intensity when it is relatively low (0.4%–2.7%), then decreases at high intensities (12.4%), and this decrease is more pronounced as the PPD increases; (iii) the drag coefficient increases with the thickness of the porous layer, and asymptotes to an effectively constant value after a critical thickness of about

1

/

8

of the cylinder’s diameter; and iv) the drag coefficient exhibits a non-monotonic dependence on the PPD of the wire mesh. These results demonstrate the importance of accounting for free-stream turbulence intensity and the parameters that characterize the porous outer layers of cylinders when modeling the drag on firebrands, or in other applications in which there is cross flow over cylinders with porous outer surfaces.
Detection of ferric ions by nitrogen and sulfur co-doped potato-derived carbon quantum dots as a fluorescent probe
Authors: Mu Miao , Duan Zhenzhen , Fan Shanshan , Zhao Wei , Gao Wenwen , Bai Rui , Li Yanjun , Kang Yuhong
Published: March 2024

This paper reports the detection of ferric ions (Fe3+) based on nitrogen and sulfur co-doped carbon quantum dots. These nitrogen and sulfur co-doped carbon quantum dots were synthesized via a hydrothermal route using northern Shaanxi potatoes as carbon sources and ammonium sulfate as nitrogen and sulfur sources. The quantum yields of the carbon quantum dots were found to be 16.96% and 4.23% with and without doping, respectively. The structural details, morphology, and optical properties of carbon quantum dots were analyzed using Fourier transform infrared spectroscopy (FT-IR), x-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy (UV–vis), and fluorescence spectroscopy. The as-prepared co-doped carbon quantum dots were utilized as a fluorescent probe for detecting Fe3+ ions, where the fluorescence intensity of carbon quantum dots was remarkably quenched in the presence of Fe3+ ions. A good linear relationship for Fe3+ ion detection was obtained from 0 to 500 μmol/L with a detection limit as low as 0.26 μmol/L. Furthermore, the proposed method also provided satisfactory results in the tap water.
Development of poly(safranine-co-phenosafranine)/GNPs/MWCNTs nanocomposites for quartz crystal microbalance sensor detection of arsenic (III) ions
Authors: Al-Sodies Salsabeel , Asiri Abdullah M , Ismail Sameh H , Alamry Khalid A , Hussein Mahmoud A
Published: March 2024

Contamination of drinking water by heavy metals is extremely dangerous to human health. The formation of a quartz crystal microbalance (QCM) sensor for the rapid and portable detection of harmful heavy metals such as arsenic (As) ions in water samples is detailed in this work. Equimolar ratios of safranine (SF) and phenosafranine (Ph) copolymers (PSF-Ph) were synthesized via a chemical oxidative polymerization approach. The copolymer was modified with multi-wall carbon nanotubes (MWCNTs) and graphene nanoplatelets (GNPs) at different percentages (1, 3, 5, and 10%) to form nanocomposites of PSF-Ph/MWCNTs/GNPs. Thermal analysis of the nanocomposites revealed that the final polymer decomposition temperature (PDTfinal) values fell between 619 and 630 °C, and the nanocomposite with 10% loading exhibited the highest decomposition temperatures for T10, T30, and T50. The nanohybrid QCM sensor detected As(III) down to parts-per-billion levels based on the change in the oscillation frequency. The sensor was tested on water samples spiked with different concentrations of As(III) (0–20 ppb). A strong linear correlation (R2 ≈ 0.99) between the frequency shift and concentration with a low detection limit (0.1 ppb) validated the quantitative detection capability of the sensor. This QCM platform with an optimal recognition ligand is a promising field-deployable tool for on-site arsenic analysis in water.
Two high-impact extreme precipitation events during the Meiyu season: simulations and their sensitivity to a scale-aware convective parameterization scheme
Authors: Qian QiFeng , Yu ZhenShou , Jia Xiaojing , Wang Dan
Published: March 2024

In this study, we investigate the impact of a scale-aware convective parameterization scheme (CPS) on simulating two high-impact extreme precipitation events during the Meiyu season at a 1 km resolution. Compared with explicit resolving simulation, applying scale-aware CPS mostly affects the atmospheric environment before convection is triggered and the regions outside the convective cell (e.g., stratiform regions) by consuming convective available potential energy (CAPE), which further inhibits precipitation. For the 2019 event, since the explicit resolving simulation underestimates precipitation, applying scale-aware CPS further inhibits precipitation and reduces the skill for the heavy rainfall category. In contrast, for the 2020 event, as the explicit resolving simulation overestimates precipitation, using scale-aware CPS inhibits precipitation and improves the skill of the model. When scale-aware CPS is applied, increasing

σ

1

,

which represents the effect of horizontal resolution, the CPS precipitation is reduced and the grid-scale precipitation is increased, but the overall effect inhibits the total precipitation. However, with the increase in

σ

1

,

although the number of stations with heavy rainfall (≥50 mm) is reduced, the average precipitation among these stations is increased. Further analysis of those stations with the largest 24 h precipitation and cross sections over regions with large radar reflectivity shows that increasing

σ

1

reduces the CAPE consumed by CPS and provides a more favorable environment for strong convections and strengthens precipitation. The results of this study may provide useful information for operational model application and may be beneficial to society.
Investigation on mechanical behavior of Al6061- coconut shell ash- granite dust reinforced hybrid metal matrix composites manufactured using stir casting technique
Authors: Mogal Yogesh K , Rane Santosh B , Mahajan Subhash K , Chaudhari Rajendra S
Published: March 2024

Owing to the superior qualities, aluminum metal matrix composites (AMMC) have recently been accepted and suggested as a replacement for traditional materials in a variety of sectors, including automotive, agriculture, aerospace, sports, marine, and many more. To satisfy the industrial requirement, it is necessary to develop economical material with higher mechanical properties like strength, hardness, impact resistance etc. To minimise the environmental impact and depletion of natural resources , it is necessary to Reduce, Recycle and Resuse the solid waste created across the world. This work aims to study the mechanical behavior of hybrid composite of Al6061 reinforced with coconut shell ash (CSA) and granite dust solid waste. Hybrid composite specimens were successfully manufactured using stir casting method. The proportion of granite dust was kept constant at 2 wt percent, whereas the percentage of CSA varied, ranging from 0% to 12% with a multiple of 3. Tests for tension, compression, hardness, and impact were performed to examine the mechanical behavior of the created hybrid composites. SEM & EDAX analysis were carried out to study the microstructure and elemental composition of the hybrid composites. Experimental testing shows that, when the percentage of CSA in the hybrid composite increased, its tensile strength, compressive strength, hardness, and impact strength increased maximum upto 23.65%, 10.12%, 60.89% and 17.38% respectively as compared to base alloy. The experimental findings demonstrated that when compared to matrix and other hybrid composites, the Al6061 alloy reinforced with 2% granite dust and 12% CSA hybrid composite had superior mechanical properties.
Hospital-treated infections and subsequent Parkinson’s disease risk: a register-based sibling comparison study
Authors: Vingeliene Snieguole , Hiyoshi Ayako , Lentjes Marleen A H , Brummer Robert J , Fall Katja , Montgomery Scott
Published: March 2024

Serious infections may result in greater risk of Parkinson’s disease. However, high-quality cohort studies focusing on a potential causal role of different types and sites of infection are lacking. Gastrointestinal infections are of a particular interest due to growing evidence implicating gut dysbiosis in Parkinson’s disease aetiology. This population-based cohort study used the Swedish Total Population Register to identify individuals born during 1944–77 and resident in Sweden between 1990 and 2018 (N = 3 698 319). Hospital-treated infections at ages 21–30 and 31–40 years were identified from the National Patient Register. Participants were followed to identify Parkinson’s disease diagnoses from age 41 years up to December 31, 2018, when the oldest individual reached 75 years. Cox regression with a sibling comparison design to tackle familial genetic and environmental confounding was used to derive hazard ratios and 95% confidence intervals for each infection site, type, or any infections at ages 21–30 and 31–40 years. During a median follow-up of 15.4 years, 8815 unique Parkinson’s disease diagnoses were accrued, with a crude rate of 17.3 (95% confidence interval 17.0, 17.7) per 100 000 person-years. After controlling for shared familial factors, hospital-treated gastrointestinal and respiratory infections between 21 and 30 years of age were associated with a greater risk of Parkinson’s disease [hazard ratios 1.35 (95% confidence interval: 1.05, 1.75) and 1.45 (95% confidence interval: 1.08, 1.95), respectively]; no association was found for any infections at age 31–40 [hazard ratio 1.05 (95% confidence interval: 0.93, 1.19)]. After adjustment, no statistically significant associations were observed for other sites including genitourinary and skin. These findings suggest that hospital-treated infections of the gastrointestinal tract and lungs, both of which may have an influence on the gut microbiome, by age 30 years may be risk factors for Parkinson’s disease.
Recognition of Governments and the Case of the Taliban
Authors: Hasar Seyfullah
Published: March 2024

This article examines the implications of the largely unexplored, yet very insightful, practice of a broad and divergent group of States concerning recognition of the Taliban, in order to contribute to the understanding of various aspects of the question of recognition of governments. Disregarding their long-standing policy of not making express statements on recognition of governments, many States put forward certain conditions for the Taliban to meet to gain recognition. Based on the arbitrary and idiosyncratic nature of these conditions, the article shows the difficulty of explaining recognition decisions by generally applicable criteria, and further establishes that the Taliban case constitutes another setback for the emerging doctrine of democratic legitimacy. Illustrating a wide range of possibilities of engagement with an unrecognised government, the article also shows that treatment, or acknowledgement, and recognition of an entity as government are two different phenomena, and demonstrates the possibility for “recognition” to be stripped of any legal or practical meaning in a given case. The article also shows that the claim that, regardless of being recognised, a general de facto government is entitled to exercise the State’s international rights and obligations does not seem well established in international law. Though some States accepted the Taliban as such, part of the international community rather alluded that the Taliban bore certain international obligations as a non-State actor, rather than on behalf of Afghanistan. The article also examines the status of unrecognised de facto governments before domestic laws, and demonstrates, among others, States’ flexibility towards such governments’ right to access State property abroad, despite the contrary national practices in the past.
Lactiplantibacillus plantarum strains KABP011, KABP012, and KABP013 modulate bile acids and cholesterol metabolism in humans
Authors: Padro Teresa , Santisteban Victoria , Huedo Pol , Puntes Montserrat , Aguiló Meritxell , Espadaler-Mazo Jordi , Badimon Lina
Published: March 2024

Aims

Probiotics with high bile salt hydrolase (BSH) activity have shown to promote cardiovascular health. However, their mechanism(s) of action remain poorly understood. Here, we performed a pilot exploratory study to investigate effects of a 4-week intervention with escalating doses of a BSH-active formula containing Lactiplantibacillus plantarum strains KABP011, KABP012, and KABP013 on bile acid (BA), lipid profile, and lipoprotein function.

Methods and results

Healthy overweight individuals were included in this study. The probiotic intake was associated with a progressive decrease of conjugated BAs in serum, due to the reduction of tauro- and glyco-conjugated forms. Plasma levels of fibroblast growth factor-19 were significantly reduced and correlated with BA changes. The probiotic induced significant changes in serum lipids, with reduction in non-HDL cholesterol (non-HDLc) and LDL cholesterol (LDLc) levels. The largest decrease was evidenced in the subgroup with higher baseline LDLc levels (LDLc > 130 mg/dL). Fasting levels of circulating apolipoprotein(Apo) B100 and ApoB48 were significantly reduced. Importantly, the decrease in non-HDLc levels was associated with a significant reduction in small LDL particles. Functional testing indicated that LDL particles had a significantly lower susceptibility to oxidation, while HDL particles gained antioxidant capacity after the probiotic intake. The microbiota profile in faeces collected at the end of the study was enriched with members of class Desulfovibrio, a taurine-consuming bacteria, likely because of the increase in free taurine in the gut due to the BSH activity of the probiotic.

Conclusion

The intervention with L. plantarum strains induces beneficial effects on BA signature and lipoprotein profile. It reduces ApoB and small LDL levels and LDL susceptibility to oxidation and increases HDL antioxidant capacity. These metabolic profile changes suggest increased protection against atherosclerotic disease.
Management of esophageal anastomotic leaks, a systematic review and network meta-analysis
Authors: Murray William , Davey Mathew G , Robb William , Donlon Noel E
Published: March 2024

There is currently no consensus as to how to manage esophageal anastomotic leaks. Intervention with endoscopic vacuum-assisted closure (EVAC), stenting, reoperation, and conservative management have all been mooted as potential options. To conduct a systematic review and network meta-analysis (NMA) to evaluate the optimal management strategy for esophageal anastomotic leaks. A systematic review was performed as per the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines with extension for NMA. NMA was performed using R packages and Shiny. In total, 12 retrospective studies were included, which included 511 patients. Of the 449 patients for whom data regarding sex was available, 371 (82.6%) were male, 78 (17.4%) were female. The average age of patients was 62.6 years (standard deviation 10.2). The stenting cohort included 245 (47.9%) patients. The EVAC cohort included 123 (24.1%) patients. The conservative cohort included 87 (17.0%) patients. The reoperation cohort included 56 (10.9%) patients. EVAC had a significantly decreased complication rate compared to stenting (odds ratio 0.23 95%, confidence interval [CI] 0.09;0.58). EVAC had a significantly lower mortality rate than stenting (odds ratio 0.43, 95% CI 0.21; 0.87). Reoperation was used in significantly larger leaks than stenting (mean difference 14.66, 95% CI 4.61;24.70). The growing use of EVAC as a first-line intervention in esophageal anastomotic leaks should continue given its proven effectiveness and significant reduction in both complication and mortality rates. Surgical management is often necessary for significantly larger leaks and will likely remain an effective option in uncontained leaks with systemic features.
Pilot study evaluating salivary bile acids as a diagnostic biomarker of laryngopharyngeal reflux
Authors: Krause Amanda J , Greytak Madeline , Kessler Marco , Yadlapati Rena
Published: March 2024

Bile acids in refluxate contribute to esophageal and laryngeal symptoms and are quantifiable. The aim of this study was to compare salivary bile acid concentrations across healthy controls and symptomatic patients (esophageal or laryngeal) with or without objective gastroesophageal reflux disease (GERD). This prospective study enrolled adults into three groups: esophageal symptoms (heartburn, regurgitation, chest pain); laryngeal symptoms (cough, throat clearing, sore throat, dysphonia); and controls. Symptomatic patients primarily underwent prolonged wireless reflux monitoring off acid suppression and were categorized as symptomatic no GERD (acid exposure time <4%) or esophageal/laryngeal symptoms with GERD (acid exposure time ≥4%). Controls did not undergo reflux monitoring nor upper endoscopy. Saliva samples were provided for bile acid analysis via ultraperformance liquid chromatography tandem mass spectrometry. Thirty-five participants were enrolled (mean age 47.4 years [SD 18.9], 16 [46%] male), including 10 controls and 25 symptomatic: 9 no GERD, 5 esophageal symptoms + GERD, and 11 laryngeal symptoms + GERD. Total salivary bile acids were highest in the laryngeal symptoms + GERD group (24.2 nM [SD 24.7]) compared to other groups (controls: 5.8 [6.0], P = 0.03; symptomatic no GERD: 3.1 [4.4]; P < 0.01; esophageal symptoms + GERD: 7.1 [7.1], P = 0.10). Bile acids were elevated in 45% (5/11) of the laryngeal symptoms + GERD group compared to 0% of the other three groups (P < 0.01). Salivary bile acids were higher among patients with laryngeal symptoms and objective GERD versus other groups. Salivary bile acids are a quantifiable biomarker with diagnostic potential for laryngopharyngeal reflux.
Research gap in esophageal achalasia: a narrative review
Authors: Savarino Edoardo Vincenzo , Salvador Renato , Ghisa Matteo , Mari Amir , Forattini Francesca , Costantini Andrea , De Giorgio Roberto , Zaninotto Giovanni
Published: March 2024

In recent years, new translational evidence, diagnostic techniques, and innovative therapies have shed new light on esophageal achalasia and revamped the attention on this relatively rare motility disorder. This narrative review aims to highlight the most recent progress and the areas where further research is needed. The four senior authors identified five topics commonly discussed in achalasia management: i.e. pathogenesis, role of functional lumen imaging probe in the diagnostic flow chart of achalasia, how to define the outcome of achalasia treatments, how to manage persistent chest pain after the treatment, and if achalasia patients’ may benefit from a regular follow-up. We searched the bibliographic databases to identify systematic reviews, meta-analyses, randomized control trials, and original research articles in English up to December 2023. We provide a summary with the most recent findings in each of the five topics and the critical points where to address future research, such as the immune-genetic patterns of achalasia that might explain the transition among the different phenotypes, the need for a validated clinical definition of treatment success, the use of neuromodulators to manage chest pain, and the need for identifying achalasia patients at risk for cancer and who may benefit of long-term follow-up. Although undoubtedly, progress has been made on the definition and management of achalasia, unmet needs remain. Debated aspects range from mechanistic insights, symptoms, objective measure relationships, and accurate clinical responses to therapeutic interventions. Translational research is eagerly awaited to answer these unresolved questions.
Left atrial appendage occlusion for recurrent stroke while on oral anticoagulation: a case series
Authors: Costa Gonçalo , Griné Mafalda , Simões Mariana , Oliveira-Santos Manuel , Paiva Luís , Costa Marco , Gonçalves Lino
Published: March 2024

Background

Clinical practice guidelines recommend oral anticoagulation (OAC) for stroke prevention in selected patients with atrial fibrillation (AF). However, some patients still experience thrombo-embolic events despite adequate anticoagulation. The optimal management of these cases remains uncertain, leading to practice pattern variability. We present a series of three cases illustrating the use of left atrial appendage occlusion (LAAO) as an adjunctive stroke prevention strategy in AF patients with recurrent thrombo-embolic events despite adequate anticoagulation.

Case summary

Case one describes an 89-year-old female on apixaban who presented with a thrombus and underwent successful mechanical thrombectomy. Left atrial appendage occlusion was performed, and no subsequent thrombo-embolic events were reported. Case 2 involves a 72-year-old female on full-dose apixaban who experienced recurrent strokes despite adequate anticoagulation. Thrombectomy was performed twice, and complications arose during LAAO. The patient was discharged on warfarin + clopidogrel and remained event-free at the six-month follow-up. Case 3 features an 88-year-old female on rivaroxaban who experienced recurrent cerebral ischaemic events and gastrointestinal bleeding. Left atrial appendage occlusion using an Amplatzer Amulet™ device was successful, and the patient remained event-free at the one-year follow-up.

Discussion

This case series emphasizes the complexity of stroke prevention in AF patients and underscores the need for an individualized approach. Incorporating LAAO alongside OAC can provide additional stroke protection for patients with inadequate response to anticoagulation. Further randomized controlled trials are needed to evaluate the efficacy and safety of this approach. In light of the limited evidence available, these cases contribute to the growing body of knowledge on the potential role of LAAO in secondary stroke prevention in AF patients with recurrent thrombo-embolic events despite appropriate anticoagulation.
Paediatric aortic valve replacement using decellularized allografts: a multicentre update following 143 implantations and five-year mean follow-up
Authors: Horke Alexander , Bobylev Dmitry , Avsar Murat , Cvitkovic Tomislav , Meyns Bart , Rega Filip , Hazekamp Mark , Cesnjevar Robert , Schmiady Martin , Staebler Brigitte , Dewald Oliver , Ciubotaru Anatol , Michel-Behnke Ina , Zimpfer Daniel , Jashari Ramadan , Boethig Dietmar , Cebotari Serghei , Beerbaum Philipp , Tudorache Igor , Sarikouch Samir
Published: March 2024

OBJECTIVES

Decellularized aortic homografts (DAH) were introduced in 2008 as a further option for paediatric aortic valve replacement (AVR).

METHODS

Prospective, multicentre follow-up of all paediatric patients receiving DAH for AVR in 8 European centres.

RESULTS

A total of 143 DAH were implanted between February 2008 and February 2023 in 137 children (106 male, 74%) with a median age of 10.8 years (interquartile range 6.6–14.6). Eighty-four (59%) had undergone previous cardiac operations and 24 (17%) had undergone previous AVR. The median implanted DAH diameter was 21 mm (interquartile range 19–23). The median operation duration was 348 min (227–439) with a median cardiopulmonary bypass time of 212 min (171–257) and a median cross-clamp time of 135 min (113–164). After a median follow-up of 5.3 years (3.3–7.2, max. 15.2 years), the primary efficacy end-points peak gradient (median 14 mmHg, 9–28) and regurgitation (median 0.5, interquartile range 0–1, grade 0–3) showed good results but an increase over time. Freedom from death/explantation/endocarditis/bleeding/thromboembolism at 5 years were 97.8 ± 1.2/88.7 ± 3.3/99.1 ± 0.9/100 and 99.2 ± 0.8%, respectively. Freedom from death/explantation/endocarditis/bleeding/thromboembolism at 10 years were 96.3 ± 1.9/67.1 ± 8.0/93.6 ± 3.9/98.6 ± 1.4 and 86.9 ± 11.6%, respectively. In total, 21 DAH were explanted. Seven were replaced by a mechanical AVR, 1 Ross operation was performed and a re-do DAH was implanted in 13 patients with no redo mortality. The calculated expected adverse events were lower for DAH compared to cryopreserved homograft patients (mean age 8.4 years), and in the same range as for Ross patients (9.2 years) and mechanical AVR (13.0 years).

CONCLUSIONS

This large-scale prospective analysis demonstrates excellent mid-term survival using DAH with adverse event rates comparable to paediatric Ross procedures.
Central precocious puberty in boys: secular trend and clinical features
Authors: Huttunen Heta , Kärkinen Juho , Varimo Tero , Miettinen Päivi J , Raivio Taneli , Hero Matti
Published: March 2024

Objective

Recent studies suggest that boys enter puberty at a younger age, and the incidence of male central precocious puberty (CPP) is increasing. In this study, we explore the incidence of male CPP and identify key clinical and auxological indicators for organic CPP (OCPP).

Design

A retrospective registry-based study.

Methods

The medical records of 43 boys treated with CPP at the Helsinki University Hospital between 1985 and 2014 were reviewed. Clinical, auxological, and endocrine data of the CPP patients were included in the analyses.

Results

Based on brain MRI, 26% of patients had OCPP. Between 2010 and 2014, the CPP incidence in boys was 0.34 per 10 000 (95% CI 0.20-0.60). Between 1990 and 2014, the male CPP incidence increased (incidence rate ratio [IRR] 1.10, P = .001). This increase was driven by rising idiopathic CPP (ICPP) incidence (IRR 1.11, 95% CI 1.05-1.19, P < .001), while OCPP incidence remained stable (P = .41). Compared with the patients with ICPP, the patients with OCPP were younger (P = .006), were shorter (P = .003), and had higher basal serum testosterone levels (P = .038). Combining 2 to 4 of these readily available clinical cues resulted in good to excellent (all, area under the curve 0.84-0.97, P < .001) overall performance, differentiating organic etiology from idiopathic.

Conclusions

The estimated incidence of CPP in boys was 0.34 per 10 000, with 26% of cases associated with intracranial pathology. The increase in CPP incidence was driven by rising ICPP rates. Patients with OCPP were characterized by shorter stature, younger age, and higher basal testosterone levels, providing valuable cues for differentiation in addition to brain MRI. Utilizing multiple cues could guide diagnostic decision-making.
Identification of different classes of genome instability suppressor genes through analysis of DNA damage response markers
Authors: Li Bin-Zhong , Kolodner Richard D , Putnam Christopher D
Published: March 2024

Cellular pathways that detect DNA damage are useful for identifying genes that suppress DNA damage, which can cause genome instability and cancer predisposition syndromes when mutated. We identified 199 high-confidence and 530 low-confidence DNA damage-suppressing (DDS) genes in Saccharomyces cerevisiae through a whole-genome screen for mutations inducing Hug1 expression, a focused screen for mutations inducing Ddc2 foci, and data from previous screens for mutations causing Rad52 foci accumulation and Rnr3 induction. We also identified 286 high-confidence and 394 low-confidence diverse genome instability-suppressing (DGIS) genes through a whole-genome screen for mutations resulting in increased gross chromosomal rearrangements and data from previous screens for mutations causing increased genome instability as assessed in a diversity of genome instability assays. Genes that suppress both pathways (DDS+ DGIS+) prevent or repair DNA replication damage and likely include genes preventing collisions between the replication and transcription machineries. DDS+ DGIS− genes, including many transcription-related genes, likely suppress damage that is normally repaired properly or prevent inappropriate signaling, whereas DDS− DGIS+ genes, like PIF1, do not suppress damage but likely promote its proper, nonmutagenic repair. Thus, induction of DNA damage markers is not a reliable indicator of increased genome instability, and the DDS and DGIS categories define mechanistically distinct groups of genes.
ClockstaRX: Testing Molecular Clock Hypotheses With Genomic Data
Authors: Duchêne David A , Duchêne Sebastián , Stiller Josefin , Heller Rasmus , Ho Simon Y W
Published: March 2024

Phylogenomic data provide valuable opportunities for studying evolutionary rates and timescales. These analyses require theoretical and statistical tools based on molecular clocks. We present ClockstaRX, a flexible platform for exploring and testing evolutionary rate signals in phylogenomic data. Here, information about evolutionary rates in branches across gene trees is placed in Euclidean space, allowing data transformation, visualization, and hypothesis testing. ClockstaRX implements formal tests for identifying groups of loci and branches that make a large contribution to patterns of rate variation. This information can then be used to test for drivers of genomic evolutionary rates or to inform models for molecular dating. Drawing on the results of a simulation study, we recommend forms of data exploration and filtering that might be useful prior to molecular-clock analyses.
Three approaches to the 1951 Convention: The case for a dialectical approach
Authors: Chimni B S
Published: March 2024

This article explores three different methodological approaches to the UN 1951 Convention on the Status of Refugees—and international refugee law (IRL) more broadly. These are termed the internal, external and dialectical approaches. It is argued that the dialectical approach, which combines elements of the internal and external approaches using a materialist postcolonial perspective helps make out in the light of changing conditions a more persuasive case for liberal interpretation and reform of the 1951 Convention. Put differently, the article is about the limitations and failings of mainstream IRL scholarship, which essentially pursues an internal approach to the 1951 Convention. It is equally about the need to decolonize and transform the pedagogy and research of IRL. This article concludes with some suggestions to advance refugee rights that would allow the 1951 Convention to respond more effectively to the protection needs of refugees around the world.
Silica-embedded Gold Nanoparticles Analyzed by Atom Probe Tomography
Authors: Eriksson Gustav , Hulander Mats , Thuvander Mattias , Andersson Martin
Published: March 2024

Nanoparticles are utilized in a multitude of applications due to their unique properties. Consequently, characterization of nanoparticles is crucial, and various methods have been employed in these pursuits. One such method is Atom Probe Tomography (APT). However, existing sample preparation techniques for APT generally involve embedding of the nanoparticles in a matrix different from their environment in solutions or at solid–liquid interfaces. In this work, we demonstrate a methodology based on silica embedding and explore how it can be utilized to form a matrix for nanoparticles suitable for APT analysis. Through chemisorption to a surface, gold nanoparticles were densely packed, ensuring a high probability of encountering at least one particle in the APT analyses. The nanoparticle-covered surface was embedded in a silica film, replacing the water and thus making this method suitable for studying nanoparticles in their hydrated state. The nanoparticle's silver content and its distribution, originating from the nanoparticle synthesis, could be identified in the APT analysis. Sodium clusters, possibly originating from the sodium citrate used to stabilize the particles in solution, were observed on the nanoparticle surfaces. This indicates the potential for silica embedding to be used for studying ligands on nanoparticles in their hydrated state.
Optimizing Vaccine Trials for Enteric Diseases: The Enterics for Global Health (EFGH) Shigella Surveillance Study
Authors: Vannice Kirsten , MacLennan Calman Alexander , Long Jessica , Steele Andrew Duncan
Published: March 2024

In this introductory article, we describe the rationale for the Enterics for Global Health (EFGH) Shigella surveillance study, which is largely to optimize the design and implementation of pivotal Shigella vaccine trials in the target population of infants and young children living in low- and middle-income countries. Such optimization will ideally lead to a shorter time to vaccine availability in the target population. We also provide a brief description of the articles included in the supplement.
The Enterics for Global Health (EFGH) Shigella Surveillance Study in Pakistan
Authors: Ahmed Naveed , Yousafzai Mohammad Tahir , Naz Qamar Farah
Published: March 2024

Background

The Enterics for Global Health (EFGH) Shigella surveillance study is a longitudinal multicountry study that aims to estimate incidence rates and document consequences of Shigella diarrhea within 7 countries in Africa, Asia, and Latin America. In addition to a high incidence of childhood diarrhea, Pakistan is facing a problem of antimicrobial resistance in urban and peri-urban areas of Karachi.

Methods

In Pakistan, EFGH will be conducted in Karachi, which is one of the metropolitan cities bordering the Arabian Sea and has a diverse population of 1.6 million according to the 2017 population census. The study aims to enroll 1400 children aged 6–35 months over 2 years (2022–2024) from 6 health care facilities (Abbasi Shaheed Hospital, Khidmat-e-Alam Medical Centre, Sindh Government Hospital Korangi 5, Sindh Government Hospital Ibrahim Hyderi, Ali Akbar Shah VPT Center, and Bhains Colony VPT Center) situated in Nazimabad and Bin-Qasim town. Moreover, population enumeration and health care utilization surveys from a defined catchment area of health facilities will be conducted to estimate the Shigella diarrhea incidence rates.

Conclusions

The study will provide critical data to policy-makers about the burden of Shigella and antimicrobial resistance, which is essential for planning Shigella vaccine trials.
The Enterics for Global Health (EFGH) Shigella Surveillance Study in Peru
Authors: Manzanares Villanueva Katia , Pinedo Vasquez Tackeshy , Peñataro Yori Pablo , Romaina Cacique Lucero , Garcia Bardales Paul F , Shapiama Lopez Wagner V , Zegarra Paredes Fiorella , Perez Karin F , Rengifo Pinedo Silvia , Silva Delgado Hermann , Flynn Thomas , Schiaffino Francesca , Colston Josh M , Paredes Olortegui Maribel Paredes , Kosek Margaret N
Published: March 2024

Background

The Enterics for Global Health (EFGH) Peru site will enroll subjects in a periurban area of the low Amazon rainforest. The political department of Loreto lags behind most of Peru in access to improved sources of water and sanitation, per capita income, children born <2.5 kg, and infant and child mortality. Chronic undernutrition as manifested by linear growth shortfalls is common, but wasting and acute malnutrition are not.

Methods

The recruitment of children seeking care for acute diarrheal disease takes place at a geographic cluster of government-based primary care centers in an area where most residents are beneficiaries of free primary healthcare.

Results

Rates of diarrheal disease, dysentery, and Shigella are known to be high in the region, with some of the highest rates of disease documented in the literature and little evidence in improvement over the last 2 decades. This study will update estimates of shigellosis by measuring the prevalence of Shigella by polymerase chain reaction and culture in children seeking care and deriving population-based estimates by measuring healthcare seeking at the community level.

Conclusions

Immunization has been offered universally against rotavirus in the region since 2009, and in a context where adequate water and sanitation are unlikely to obtain high standards in the near future, control of principal enteropathogens through immunization may be the most feasible way to decrease the high burden of disease in the area in the near future.
The Enterics for Global Health (EFGH) Shigella Surveillance Study in Malawi
Authors: Mategula Donnie , Ndalama Maureen , Lefu Clement , Chinkhumba Jobiba , Ndeketa Latif , Munthali Vitumbiko , Chitala Clifford , Malemia Thandizo , Million Gertrude , Mbutuka Ishmail , Mhone Ranken , Makwenda Ethel , James Mussa , Bwanali Cornelius , Kazembe Gift , Manundo Abell , Chauluka Evance , Chitalo Salama , Alumando Ethel , Longwe Dalitso , Matandika Maggie , Jonasi Paul , Thindwa Agra , Phiri Deborah , Wachepa Richard , Kawonga Flywell , Maiden Victor , Charles Mary , Kapindula Ida , Witte Desiree , Turner Ann M , Bronowski Christina , Baker Kate , Bar-Zeev Naor , Gordon Melita A , Dube Queen , Cunliffe Nigel A , Jere Khuzwayo C , Cornick Jennifer
Published: March 2024

Background

Malawi is among 7 countries participating in the Enterics for Global Health (EFGH) Shigella surveillance study, which aims to determine the incidence of medically attended diarrhea attributed to Shigella, a leading bacterial cause of diarrhea in children in low-resource settings.

Methods

We describe the EFGH study site in the densely populated informal settlement of Ndirande Township, Blantyre, Malawi. We explore the site’s geographical location, demographic characteristics, and the healthcare-seeking behavior of its population, particularly for childhood diarrhea. We also describe the management of childhood diarrhea at the health facility, and the associated challenges to attaining optimum adherence to local and national guidelines at the site.

Conclusions

Our overarching aim is to improve global health through understanding and mitigating the impact of diarrhea attributed to Shigella.
Gelatinous bone marrow with vivid fatty cells
Authors: Imataki Osamu , Uemura Makiko
Published: March 2024

We treated a 78-year-old Japanese man with gastric diffuse large B cell lymphoma. The patient received three courses of chemotherapy and involved-field local radiation therapy to the stomach. Three years after chemotherapy, the patient become sick with anorexia and pancytopenia. He was cachexic, however, levels of vitamin B12, folate, zinc, and copper were normal. His bone marrow revealed focal eosinophilic deposit characterized by mixture of vivid atrophic fatty cells, which was pathologically diagnosed as gelatinous bone marrow. The patient’s gelatinous marrow was characterized by CD68-stained vivid atrophic fatty tissue. We found that this finding is a possible alternative marker for gelatinous marrow.
A malignant spiradenoma of the forehead: a case report and literature review
Authors: Ahmed Mohamed Badie , Elzawawi Khaled E , AlHammadi Ayda , Al-Malki Aysha , Petkar Mahir , Al Lahham Salim , Alsherawi Abeer
Published: March 2024

Spiroadenomas, arising from sweat glands, are rare benign skin tumors primarily found as solitary nodules on the head, neck, and trunk. The malignant subtype, Spiradenocarcinoma (MSA), originating from benign spiradenomas, is extremely rare and occurs mainly in individuals over 50. MSA exhibits aggressiveness with higher metastasis rates and lower survival rates. Surgical excision is the standard management, supported by imaging modalities like MRI, CT-scan, and ultrasound. We present a 69-year-old female with a medical history of diabetes, hypertension, and dyslipidemia who presented in 2014 with multiple swellings on the forehead and left arm. Initial excisions revealed capillary hemangioma lesions. Subsequent visits involved the excision of further facial and body lesions, with some identified as intradermal nevi. In 2022, she presented to plastic surgery clinic with forehead swelling. The biopsy showed MSA lesion with involved margins. Thus, subsequent re-excision was carried out. One year later, she came with recurrent forehead swelling. Excision and direct closure of the lesion showed involvement of part of the subcutaneous tissue (fat globule) showing residual/recurrent MSA that is very close to nerve trunks. Malignant Spiradenomas (MSAs) usually arise from benign spiradenomas. Therefore, consideration is given to preemptive removal of these tumors due to their potential evolution. The primary treatment approach involves surgery, with a focus on wide local excision and a minimum margin of 1 cm to diminish the risk of metastasis. Vigilant follow-up is essential to promptly identify any recurrences or spreading.

  What’s already known about this topic? Malignant spiradenoma is an exceedingly uncommon malignant skin adnexal tumor. The primary approach is surgical, involving wide local excision with a margin of at least 1 cm. What does this report add? Highlights the importance of suspecting these lesions early to avoid local metastasis. Shed light on the importance of follow-up visits to be able to monitor the lesion progression or recurrence after excision.
Transient recurrent episodes of abducens nerve palsy and cheiro-oral syndrome in a sub-occlusive carotid bulb atherosclerotic stenosis
Authors: Toudou-Daouda Moussa , Yatwa-Zaniwe Roger-Venant , Aminou-Tassiou Nana-Rahamatou , Chausson Nicolas , Smadja Didier
Published: March 2024

We report the case of a male in his 50s with a history of smoking admitted to our hospital for three transient recurrent episodes of less than 60 min of cheiro-oral paresthesias and binocular horizontal diplopia with convergent strabismus. On admission, his neurological examination was normal. Cerebral magnetic resonance imaging showed no cerebral lesion. Computed tomography angiography showed a sub-occlusive right carotid bulb atherosclerotic stenosis, the absence of abnormality of the subclavian arteries and the origin of the vertebral arteries, and no stenosis of the basilar artery or posterior cerebral arteries. Routine blood tests were normal with glycated hemoglobin of 6.5%. The patient underwent right carotid endarterectomy. One year after carotid endarterectomy, the patient has had no other cerebrovascular events.
Udon enteropathy
Authors: Imataki Osamu , Uemura Makiko
Published: March 2024

Japanese noodle ‘udon’ is an original Asian noodle popular in Japan. Udon is one of the most elastic noodle, and which is required to take more than several hours to digest in a certain case. We encountered a case of enteropathy caused by consumption of udon. A fifteen-year-old girl visited the outpatient clinic with a symptom of epigastralgia persistent from the previous night. She had eaten noodles for dinner the night before. Clinical images showed patient’s stomach filled with udon. Noodle-associated enteropathy is not rare in our community, however, there had not been reported in other Asian countries.
The adaptor protein ECAP, the corepressor LEUNIG, and the transcription factor BEH3 interact and regulate microsporocyte generation in Arabidopsis
Authors: Shi Lei , Li Changjiang , Lv Gaofeng , Li Xing , Feng Wutao , Bi Yujing , Wang Wenhui , Wang Youqun , Zhu Lei , Tang Wenqiang , Fu Ying
Published: March 2024

Histospecification and morphogenesis of anthers during development in Arabidopsis (Arabidopsis thaliana) are well understood. However, the regulatory mechanism of microsporocyte generation at the pre-meiotic stage remains unclear, especially how archesporial cells are specified and differentiate into 2 cell lineages with distinct developmental fates. SPOROCYTELESS (SPL) is a key reproductive gene that is activated during early anther development and remains active. In this study, we demonstrated that the EAR motif-containing adaptor protein (ECAP) interacts with the Gro/Tup1 family corepressor LEUNIG (LUG) and the BES1/BZR1 HOMOLOG3 (BEH3) transcription factor to form a transcription activator complex, epigenetically regulating SPL transcription. SPL participates in microsporocyte generation by modulating the specification of archesporial cells and the archesporial cell–derived differentiation of somatic and reproductive cell layers. This study illustrates the regulation of SPL expression by the ECAP–LUG–BEH3 complex, which is essential for the generation of microsporocytes. Moreover, our findings identified ECAP as a key transcription regulator that can combine with different partners to regulate gene expression in distinct ways, thereby facilitating diverse processes in various aspects of plant development.
Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel
Authors: Zhu Delong , Hu Ying , Kong Xiangkai , Luo Yuansen , Zhang Yi , Wu Yu , Tan Jiameng , Chen Jianwei , Xu Tao , Zhu Lei
Published: March 2024

Adipose mesenchymal stem cell (ADMSC)-derived exosomes (ADMSC-Exos) have shown great potential in regenerative medicine and been evidenced benefiting wound repair such as burns. However, the low yield, easy loss after direct coating, and no suitable loading system to improve their availability and efficacy hinder their clinical application for wound healing. And few studies focused on the comparison of biological functions between exosomes derived from different culture techniques, especially in exosome-releasing hydrogel system. Therefore, we designed a high-performance exosome controllable releasing hydrogel system for burn wound healing, namely loading 3D-printed microfiber culture-derived exosomes in a highly biocompatible hyaluronic acid (HA). In this project, we compared the biological functions in vitro and in a burn model among exosomes derived from the conventional two-dimensional (2D) plate culture (2D-Exos), microcarrier culture (2.5D-Exos), and 3D-printed microfiber culture (3D-Exos). Results showed that compared with 2D-Exos and 2.5D-Exos, 3D-Exos promoted HACATs and HUVECs cell proliferation and migration more significantly. Additionally, 3D-Exos had stronger angiogenesis-promoting effects in tube formation of (HUVECs) cells. Moreover, we found HA-loaded 3D-Exos showed better burn wound healing promotion compared to 2D-Exos and 2.5D-Exos, including accelerated burn wound healing rate and better collagen remodeling. The study findings reveal that the HA-loaded, controllable-release 3D-Exos repair system distinctly augments therapeutic efficacy in terms of wound healing, while concurrently introducing a facile application approach. This system markedly bolsters the exosomal loading efficiency, provides a robust protective milieu, and potentiates the inherent biological functionalities of the exosomes. Our findings provide a rationale for more efficient utilization of high-quality and high-yield 3D exosomes in the future, and a novel strategy for healing severe burns.