bluuebunny/crossref_metadata_2025 huggingface dataset:
Seam Properties of Overlap Welding Strategies from Copper to Aluminum Using Green Laser Radiation for Battery Tab Connections in Electric Vehicles
Authors: Kaufmann Florian , Strugulea Mihail , Höltgen Christian , Roth Stephan , Schmidt Michael
Published: January 2023

Laser beam welding of metals has progressed dramatically over the last years mainly arising from joining applications in the field of electromobility. Allowing the flexible, automated manufacturing of mechanically, electrically, and thermally stressed components, the process is more frequently applied for joining highly reflective materials, for example for battery tab and busbar connections. The local, non-contact energy input favors this welding technology; however, joining of copper and aluminum sheets still poses a challenge due to the physical properties of the joining partners and intermetallic phases from dissimilar metal interaction, which reduce seam performance. The use of green laser radiation compared to infrared laser radiation offers the advantage of a significantly increased absorptivity for copper materials. A changed incoupling behavior is observed, and a lower deep penetration threshold has been already proven for 515 nm wavelength. When copper and aluminum are welded with the former as top sheet, this welding mode is essential to overcome limited aspect ratios from heat conduction welding. However, the opportunities of applying these beam sources in combination with spatial power modulation to influence the interconnection area of copper-aluminum joints have not yet been studied. The aim of this work is therefore to investigate the seam properties and process stability of different overlap welding strategies using green laser radiation for dissimilar metal welding. A microstructural analysis of the different fusion zones and mechanical strength of the joints are presented. In addition, the experimental parameter sets were analyzed regarding their application in battery module busbars by examining the electrical resistance and temperature distribution after welding. A parameter window was identified for all investigated welding strategies, with the stitched seam achieving the most stable results.
Prediction Model of Elderly Care Willingness Based on Machine Learning
Authors: Jin Yongchao , Liu Dongmei , Wang Kenan , Wang Renfang , Zhuang Xiaodie
Published: January 2023

At present, the problem of an aging population in China is severe. The integration of existing healthcare services with elderly care services is inefficient and cannot meet the needs of the elderly. As such, China urgently needs the concerted efforts of various social forces to cope with the increasingly serious problem of aging. In accordance with Andersen’s behavioral model, a survey was conducted in Tangshan City among seniors 60 years of age and older. Using logistic regression models, decision tree models, and random forest models, we examined the factors impacting senior people’s desire to choose the integrated medical care and nursing care model. The results of the three models displayed that the elderly’s propensity to choose the combined medical care and nursing care model is significantly influenced by the amount of insurance, life care needs, and healthcare needs. Moreover, the study found that the willingness of the elderly in Tangshan to improve the combined medical and nursing care service system is low. The government should appeal to the community to participate in multiple developments to improve the integrated medical and nursing service system.
Overview of Identification Methods of Autoregressive Model in Presence of Additive Noise
Authors: Ivanov Dmitriy , Yakoub Zaineb
Published: January 2023

This paper presents an overview of the main methods used to identify autoregressive models with additive noises. The classification of identification methods is given. For each group of methods, advantages and disadvantages are indicated. The article presents the simulation results of a large number of the described methods and gives recommendations on choosing the best methods.
Research on the 2A11 Aluminum Alloy Sheet Cyclic Tension–Compression Test and Its Application in a Mixed Hardening Model
Authors: Chen Guang , Zhao Changcai , Shi Haiwei , Zhu Qingxing , Shen Guoyi , Liu Zheng , Wang Chenyang , Chen Duan
Published: January 2023

The increasing application of aluminum alloy, in combination with the growth in the complexity of components, provides new challenges for the numerical modeling of sheet materials. The material elastic–plasticity constitutive model is the most important factor affecting the accuracy of finite element simulation. The mixed hardening constitutive model can more accurately represent the real hardening characteristics of the material plastic deformation process, and the accuracy of the material property-related parameters in the constitutive model directly affects the accuracy of finite element simulation. Based on the Hill48 anisotropic yield criterion, combined with the Voce isotropic hardening model and the Armstrong–Frederic nonlinear kinematic hardening model, a mixed hardening constitutive model that considers material anisotropy and the Bauschinger effect was established. Analysis of the tension–compression experiment on the sheet using finite element method. Using the finite element model, the optimum geometry of the tension–compression experiment sample was determined. The cyclic deformation stress–strain curve of the 2A11 aluminum alloy sheet was obtained by a cyclic tensile–compression test, and the material characteristic parameters in the mixed hardening model were accurately determined. The reliability and accuracy of the established constitutive model of anisotropic mixed hardening materials were verified by the finite element simulation and by testing the cyclic tensile–compression problem, the springback problem, and the sheet in bending, unloading, and reverse bending problems. The tensile–compression experiment is an effective method to directly and accurately obtain the characteristic parameters of constitutive model materials.
Effects of a Maximal Exercise Followed by a Submaximal Exercise Performed in Normobaric Hypoxia (2500 m), on Blood Rheology, Red Blood Cell Senescence, and Coagulation in Well-Trained Cyclists
Authors: Carin Romain , Deglicourt Gabriel , Rezigue Hamdi , Martin Marie , Nougier Christophe , Boisson Camille , Dargaud Yesim , Joly Philippe , Renoux Céline , Connes Philippe , Stauffer Emeric , Nader Elie
Published: January 2023

Acute normoxic exercise impacts the rheological properties of red blood cells (RBC) and their senescence state; however, there is a lack of data on the effects of exercise performed in hypoxia on RBC properties. This crossover study compared the effects of acute hypoxia vs. normoxia on blood rheology, RBC senescence, and coagulation during exercise. Nine trained male cyclists completed both a session in normoxia (FiO2 = 21%) and hypoxia (FiO2 = 15.3% ≈ 2500 m). The two sessions were randomly performed, separated by one week, and consisted of an incremental and maximal exercise followed by a 20 min exercise at the first ventilatory threshold (VT1) on a home-trainer. Blood samples were taken before and after exercise to analyze hematological parameters, blood rheology (hematocrit, blood viscosity, RBC deformability and aggregation), RBC senescence markers (phosphatidylserine (PS) and CD47 exposure, intraerythrocyte reactive oxygen species (ROS), and calcium content), and blood clot viscoelastic properties. Hemoglobin oxygen saturation (SpO2) and blood lactate were also measured. In both conditions, exercise induced an increase in blood viscosity, hematocrit, intraerythrocyte calcium and ROS content, and blood lactate concentration. We also observed an increase in blood clot amplitude, and a significant drop in SpO2 during exercise in the two conditions. RBC aggregation and CD47 exposure were not modified. Exercise in hypoxia induced a slight decrease in RBC deformability which could be related to the slight increase in mean corpuscular hemoglobin concentration (MCHC). However, the values of RBC deformability and MCHC after the exercise performed in hypoxia remained in the normal range of values. In conclusion, acute hypoxia does not amplify the RBC and coagulation changes induced by an exercise bout.
Area-Efficient Mapping of Convolutional Neural Networks to Memristor Crossbars Using Sub-Image Partitioning
Authors: Oh Seokjin , An Jiyong , Min Kyeong-Sik
Published: January 2023

Memristor crossbars can be very useful for realizing edge-intelligence hardware, because the neural networks implemented by memristor crossbars can save significantly more computing energy and layout area than the conventional CMOS (complementary metal–oxide–semiconductor) digital circuits. One of the important operations used in neural networks is convolution. For performing the convolution by memristor crossbars, the full image should be partitioned into several sub-images. By doing so, each sub-image convolution can be mapped to small-size unit crossbars, of which the size should be defined as 128 × 128 or 256 × 256 to avoid the line resistance problem caused from large-size crossbars. In this paper, various convolution schemes with 3D, 2D, and 1D kernels are analyzed and compared in terms of neural network’s performance and overlapping overhead. The neural network’s simulation indicates that the 2D + 1D kernels can perform the sub-image convolution using a much smaller number of unit crossbars with less rate loss than the 3D kernels. When the CIFAR-10 dataset is tested, the mapping of sub-image convolution of 2D + 1D kernels to crossbars shows that the number of unit crossbars can be reduced almost by 90% and 95%, respectively, for 128 × 128 and 256 × 256 crossbars, compared with the 3D kernels. On the contrary, the rate loss of 2D + 1D kernels can be less than 2%. To improve the neural network’s performance more, the 2D + 1D kernels can be combined with 3D kernels in one neural network. When the normalized ratio of 2D + 1D layers is around 0.5, the neural network’s performance indicates very little rate loss compared to when the normalized ratio of 2D + 1D layers is zero. However, the number of unit crossbars for the normalized ratio = 0.5 can be reduced by half compared with that for the normalized ratio = 0.
A Simple Model of the Energy Harvester within a Linear and Hysteresis Approach
Authors: Semenov Mikhail E. , Meleshenko Peter A. , Borzunov Sergei V. , Reshetova Olga O. , Barsukov Andrey I.
Published: January 2023

In this article, a model of an energy harvester, the mechanical part of which is an inverted pendulum, is proposed. We investigated the stability of a linearized system. It was proven that the stabilizing control of the pendulum, based on the feedback principle, enables the stabilization of the system. We have identified the zones of stability and the amplitude–frequency characteristics. In the second part of this article, a generalization of the dynamic system for the case of the hysteresis friction in the mechanical joint is considered. The role of nonlinear effects within the design Preisach model and the phenomenological Bouc–Wen model is shown.
Design of a Microflyer Driven by a Microsized Charge Combined with an Initiation Criterion
Authors: He Xiang , Yang Lixin , Dong Haiping , Lv Zhixing , Yan Nan
Published: January 2023

In order to study the performance of ultra-fine 2,2′, 4,4′, 6,6′–hexanitrostilbene (HNS-IV) explosives initiated by a microflyer driven by microsized lead azide (Pb(N3)2), a corresponding simulation model was established in Autodyn software, and the accuracy of the simulation model was verified with a photonic Doppler velocimeter (PDV). Various influencing factors were studied in combination with the power flux–action time (Π-τ) initiation criterion. The results showed that the exponential growth rate of the flyer velocity decreased with an increase in the diameter and height of the lead azide and that the influence of the charge diameter was more obvious than that of the charge height. The flyer velocity increased linearly with the density of the lead azide. The velocity of the flyer also increased linearly with an increase in the shock wave impedance of the restraint materials, and the velocities of the flyer that corresponded to silicon and organic glass were lower than those of the metal materials. The flyer’s velocity and power flux increased with a decrease in the flyer’s density; when considering the flyer’s velocity, power flux, and actual shear effect, titanium was the best material for the flyer. As the thickness of the flyer was decreased, the velocity and power flux of the flyer increased; under the premise of satisfying the forming effect, the thinner flyer was selected. When used as the material for the acceleration chamber, silicon showed a lower flyer velocity and power flux than sapphire, nickel, stainless steel, and other materials. With the increase in the acceleration chamber aperture, the exponentially declining trend in the flyer’s velocity increased; when the aperture of the accelerating chamber was consistent with the diameter of the primary explosive, the power flux was the largest. Finally, the ability of the microflyer to initiate the HNS-IV was verified by a steel dent test.
Geochemistry of Brine and Paleoclimate Reconstruction during Sedimentation of Messinian Salt in the Tuz Gölü Basin (Türkiye): Insights from the Study of Fluid Inclusions
Authors: Galamay Anatoliy R. , Karakaya Muazzez Çelik , Bukowski Krzysztof , Karakaya Necati , Yaremchuk Yaroslava
Published: January 2023

The halogenesis of the Messinian Tuz Gölü Basin corresponds to the sulfate type and the magnesium sulfate subtype. Compared to the Messinian Sea brines, they have a slightly higher [Na+] concentration, which is 96.6–116.4 g/L, and a much lower [K+] concentration, ranging from 0.1 to 2.35 g/L. During salt sedimentation, the [Mg2+] concentration ranged from 6.1 to 14.0 g/L, and the [SO42−] concentration from 18.2 to 4.5 g/L. Physical–chemical reactions in the basin’s near-surface and bottom waters during the suspension of halite deposition had a decisive influence on the significant reduction of [SO42−] sedimentation brines. During these periods, there was an intensive influx of Ca(HCO3)2 into the sedimentation basin and the formation of glauberite layers. The formation of the glauberite resulted from the slow dissolution of pre-deposited finely dispersed metastable minerals—gypsum, sodium syngenite, or mirabilite. In fluid inclusions in the halite, the sulfate minerals being allogenic crystals of calcium sulfate, are represented by gypsum, bassanite, and anhydrite. Additionally, as the other sulfate minerals, glauberite, anhydrite, and thenardite are found within halite crystals. Sharp fluctuations in daytime air temperatures characterized climatic indicators of the summer period in the Tuz Gölü region: 15.6–49.1 °C. In the spring or cool summer–autumn period, the daytime air temperature in the region ranged from 15.7–22.1 °C, and in late spring and early summer, it ranged from 20.6 °C to 35.0 °C. During some periods, the Tuz Gölü halite crystallized at 61.8–73.5 °C. The extreme high-temperature crystallization regime at the bottom of the salt-bearing basin was achieved due to the emergence of a vertical thermohaline structure. The “greenhouse effect” in the Tuz Gölü was established only briefly but was periodically renewed due to the influx of “fresh” waters.
Lutein Induces Reactive Oxygen Species-Mediated Apoptosis in Gastric Cancer AGS Cells via NADPH Oxidase Activation
Authors: Eom Ju Won , Lim Joo Weon , Kim Hyeyoung
Published: January 2023

Disruption of apoptosis leads to cancer cell progression; thus, anticancer agents target apoptosis of cancer cells. Reactive oxygen species (ROS) induce apoptosis by activating caspases and caspase-dependent DNase, leading to DNA fragmentation. ROS increase the expression of apoptotic protein Bax, which is mediated by activation of nuclear factor-κB (NF--κB). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is an important source of endogenous ROS, and its activation is involved in apoptosis. Lutein, an oxygenated carotenoid and known antioxidant, is abundant in leafy dark green vegetables, such as spinach and kale, and in yellow-colored foods, such as corn and egg yolk. High amounts of lutein increase ROS levels and exhibit anticancer activity. However, its anticancer mechanism remains unclear. This study aimed to determine whether lutein activates NADPH oxidase to produce ROS and induce apoptosis in gastric cancer AGS cells. Lutein increased ROS levels and promoted the activation of NADPH oxidase by increasing the translocation of NADPH oxidase subunit p47 phox to the cell membrane. It increased NF-κB activation and apoptotic indices, such as Bax, caspase-3 cleavage, and DNA fragmentation, and decreased Bcl-2, cell viability, and colony formation in AGS cells. The specific NADPH oxidase inhibitor ML171, and the known antioxidant N-acetyl cysteine reversed lutein-induced cell death, DNA fragmentation, and NF-κB DNA-binding activity in AGS cells. These results suggest that lutein-induced ROS production is dependent on NADPH oxidase, which mediates NF-κB activation and apoptosis in gastric cancer AGS cells. Therefore, lutein supplementation may be beneficial for increasing ROS-mediated apoptosis in gastric cancer cells.
The Versatile Photo-Thermal Behaviour of a 2-Hydroxyazobenzene
Authors: Gentili Pier Luigi , Capaccioni Antonio , Germani Raimondo , Fantacci Simona
Published: January 2023

Photochromic compounds are employed in implementing neuron surrogates. They will boost the development of neuromorphic engineering in wetware. In this work, the photochromic behaviours of (E)-3,4,6-trichloro-2-(p-diazenil)-phenol (t-DZH) and its conjugated phenoxide base (t-DZ) have been investigated experimentally in three different media: (1) pure acetonitrile, (2) in water and acetonitrile mixed in a 1/1 volume ratio, and (3) in an aqueous micellar solution of 3-(N,N-Dimethylmyristylammonio)propanesulfonate (SB3-14). The analysis of the spectral and kinetic features of t-DZH and t-DZ has been supported by quantum-mechanical DFT calculations, the maximum entropy method, and the determination of their colourability (C). The versatility of t-DZH and t-DZ makes them promising molecular probes of micro-environments and potential ingredients of photochemical oscillators required for implementing pacemaker neurons capable of communicating through optical signals in wetware.
Chlamydomonas reinhardtii: A Factory of Nutraceutical and Food Supplements for Human Health
Authors: Masi Annalisa , Leonelli Francesca , Scognamiglio Viviana , Gasperuzzo Giulia , Antonacci Amina , Terzidis Michael A.
Published: January 2023

Chlamydomonas reinhardtii (C. reinhardtii) is one of the most well-studied microalgae organisms that revealed important information for the photosynthetic and metabolic processes of plants and eukaryotes. Numerous extensive studies have also underpinned its great potential as a biochemical factory, capable of producing various highly desired molecules with a direct impact on human health and longevity. Polysaccharides, lipids, functional proteins, pigments, hormones, vaccines, and antibodies are among the valuable biomolecules that are produced spontaneously or under well-defined conditions by C. reinhardtii and can be directly linked to human nutrition and diet. The aim of this review is to highlight the recent advances in the field focusing on the most relevant applications related to the production of important biomolecules for human health that are also linked with human nutrition and diet. The limitations and challenges are critically discussed along with the potential future applications of C. reinhardtii biomass and processed products in the field of nutraceuticals and food supplements. The increasing need for high-value and low-cost biomolecules produced in an environmentally and economy sustainable manner also underline the important role of C. reinhardtii.
Influence of Substituents in a Six-Membered Chelate Ring of HG-Type Complexes Containing an N→Ru Bond on Their Stability and Catalytic Activity
Authors: Vasilyev Kirill A. , Antonova Alexandra S. , Volchkov Nikita S. , Logvinenko Nikita A. , Nikitina Eugeniya V. , Grigoriev Mikhail S. , Novikov Anton P. , Kouznetsov Vladimir V. , Polyanskii Kirill B. , Zubkov Fedor I.
Published: January 2023

An efficient approach to the synthesis of olefin metathesis HG-type catalysts containing an N→Ru bond in a six-membered chelate ring was proposed. For the most part, these ruthenium chelates can be prepared easily and in high yields based on the interaction between 2-vinylbenzylamines and Ind II (the common precursor for Ru-complex synthesis). It was demonstrated that the increase of the steric volume of substituents attached to the nitrogen atom and in the α-position of the benzylidene fragment leads to a dramatic decrease in the stability of the target ruthenium complexes. The bulkiest iPr substituent bonded to the nitrogen atom or to the α-position does not allow the closing of the chelate cycle. N,N-Diethyl-1-(2-vinylphenyl)propan-1-amine is a limiting case; its interaction with Ind II makes it possible to isolate the corresponding ruthenium chelate in a low yield (5%). Catalytic activity of the synthesized complexes was tested in RCM reactions and compared with α-unsubstituted catalysts obtained previously. The structural peculiarities of the final ruthenium complexes were thoroughly investigated using XRD and NMR analysis, which allowed making a reliable correlation between the structure of the complexes and their catalytic properties.
An Update on the Therapeutic Anticancer Potential of Ocimum sanctum L.: “Elixir of Life”
Authors: Hasan Mohammad Raghibul , Alotaibi Bader Saud , Althafar Ziyad Mohammed , Mujamammi Ahmed Hussain , Jameela Jafar
Published: January 2023

In most cases, cancer develops due to abnormal cell growth and subsequent tumour formation. Due to significant constraints with current treatments, natural compounds are being explored as potential alternatives. There are now around 30 natural compounds under clinical trials for the treatment of cancer. Tulsi, or Holy Basil, of the genus Ocimum, is one of the most widely available and cost-effective medicinal plants. In India, the tulsi plant has deep religious and medicinal significance. Tulsi essential oil contains a valuable source of bioactive compounds, such as camphor, eucalyptol, eugenol, alpha-bisabolene, beta-bisabolene, and beta-caryophyllene. These compounds are proposed to be responsible for the antimicrobial properties of the leaf extracts. The anticancer effects of tulsi (Ocimum sanctum L.) have earned it the title of “queen of herbs” and “Elixir of Life” in Ayurvedic treatment. Tulsi leaves, which have high concentrations of eugenol, have been shown to have anticancer properties. In a various cancers, eugenol exerts its antitumour effects through a number of different mechanisms. In light of this, the current review focuses on the anticancer benefits of tulsi and its primary phytoconstituent, eugenol, as apotential therapeutic agent against a wide range of cancer types. In recent years, tulsi has gained popularity due to its anticancer properties. In ongoing clinical trials, a number of tulsi plant compounds are being evaluated for their potential anticancer effects. This article discusses anticancer, chemopreventive, and antioxidant effects of tulsi.
Multivariate Chemometric Comparison of Forced Degradation and Electrochemical Oxidation LC–MS Profiles of Maraviroc
Authors: Wroński Michał , Trawiński Jakub , Komsta Łukasz , Skibiński Robert
Published: January 2023

In this study, nine forced degradation products of maraviroc were found using chemometric analysis. This antiretroviral drug was subjected to photolytic, oxidative, as well as neutral, basic and acidic hydrolysis stress conditions. Additionally, its electrochemical transformation on platinum, gold and glassy carbon screen-printed electrodes was examined. This study showed that maraviroc is especially susceptible to UVA, H2O2 and electrochemical degradation, while being resistant to neutral and acidic hydrolysis. A cluster analysis showed that the electrochemical transformation, with particular reference to the platinum electrode, is able to partially simulate the forced degradation processes, especially in the context of redox reactions. These findings indicate that the electrochemical methods can be considered as quick and relatively low-cost supplements to the commonly applied forced degradation procedures.
Synthesis and Antiproliferative Activity of Steroidal Diaryl Ethers
Authors: Kovács Édua , Ali Hazhmat , Minorics Renáta , Traj Péter , Resch Vivien , Paragi Gábor , Bruszel Bella , Zupkó István , Mernyák Erzsébet
Published: January 2023

Novel 13α-estrone derivatives have been synthesized via direct arylation of the phenolic hydroxy function. Chan–Lam couplings of arylboronic acids with 13α-estrone as a nucleophilic partner were carried out under copper catalysis. The antiproliferative activities of the newly synthesized diaryl ethers against a panel of human cancer cell lines (A2780, MCF-7, MDA-MB 231, HeLa, SiHa) were investigated by means of MTT assays. The quinoline derivative displayed substantial antiproliferative activity against MCF-7 and HeLa cell lines with low micromolar IC50 values. Disturbance of tubulin polymerization has been confirmed by microplate-based photometric assay. Computational calculations reveal significant interactions of the quinoline derivative with the taxoid binding site of tubulin.
Bioactive Clerodane Diterpenoids from the Leaves of Casearia coriacea Vent
Authors: Ledoux Allison , Hamann Carla , Bonnet Olivier , Jullien Kateline , Quetin-Leclercq Joëlle , Tchinda Alembert , Smadja Jacqueline , Gauvin-Bialecki Anne , Maquoi Erik , Frédérich Michel
Published: January 2023

Casearia coriacea Vent., an endemic plant from the Mascarene Islands, was investigated following its antiplasmodial potentialities highlighted during a previous screening. Three clerodane diterpene compounds were isolated and identified as being responsible for the antiplasmodial activity of the leaves of the plant: caseamembrin T (1), corybulosin I (2), and isocaseamembrin E (3), which exhibited half maximal inhibitory concentrations (IC50) of 0.25 to 0.51 µg/mL. These compounds were tested on two other parasites, Leishmania mexicana mexicana and Trypanosoma brucei brucei, to identify possible selectivity in one of them. Although these products possess both antileishmanial and antitrypanosomal properties, they displayed selectivity for the malaria parasite, with a selectivity index between 6 and 12 regarding antitrypanosomal activity and between 25 and 100 regarding antileishmanial activity. These compounds were tested on three cell lines, breast cancer cells MDA-MB-231, pulmonary adenocarcinoma cells A549, and pancreatic carcinoma cells PANC-1, to evaluate their selectivity towards Plasmodium. This has not enabled us to establish selectivity for Plasmodium, but has revealed the promising activity of compounds 1–3 (IC50 < 2 µg/mL), particularly against pancreatic carcinoma cells (IC50 < 1 µg/mL). The toxicity of the main compound, caseamembrin T (1), was then evaluated on zebrafish embryos to extend our cytotoxicity study to normal, non-cancerous cells. This highlighted the non-negligible toxicity of caseamembrin T (1).
Hyperpolarizing DNA Nucleobases via NMR Signal Amplification by Reversible Exchange
Authors: Kidd Bryce E. , Gemeinhardt Max E. , Mashni Jamil A. , Gesiorski Jonathan L. , Bales Liana B. , Limbach Miranda N. , Shchepin Roman V. , Kovtunov Kirill V. , Koptyug Igor V. , Chekmenev Eduard Y. , Goodson Boyd M.
Published: January 2023

The present work investigates the potential for enhancing the NMR signals of DNA nucleobases by parahydrogen-based hyperpolarization. Signal amplification by reversible exchange (SABRE) and SABRE in Shield Enables Alignment Transfer to Heteronuclei (SABRE-SHEATH) of selected DNA nucleobases is demonstrated with the enhancement (ε) of 1H, 15N, and/or 13C spins in 3-methyladenine, cytosine, and 6-O-guanine. Solutions of the standard SABRE homogenous catalyst Ir(1,5-cyclooctadeine)(1,3-bis(2,4,6-trimethylphenyl)imidazolium)Cl (“IrIMes”) and a given nucleobase in deuterated ethanol/water solutions yielded low 1H ε values (≤10), likely reflecting weak catalyst binding. However, we achieved natural-abundance enhancement of 15N signals for 3-methyladenine of ~3300 and ~1900 for the imidazole ring nitrogen atoms. 1H and 15N 3-methyladenine studies revealed that methylation of adenine affords preferential binding of the imidazole ring over the pyrimidine ring. Interestingly, signal enhancements (ε~240) of both 15N atoms for doubly labelled cytosine reveal the preferential binding of specific tautomer(s), thus giving insight into the matching of polarization-transfer and tautomerization time scales. 13C enhancements of up to nearly 50-fold were also obtained for this cytosine isotopomer. These efforts may enable the future investigation of processes underlying cellular function and/or dysfunction, including how DNA nucleobase tautomerization influences mismatching in base-pairing.
Cost-Effective and Facile Fabrication of a Tattoo Paper-Based SERS Substrate and Its Application in Pesticide Sensing on Fruit Surfaces
Authors: Mandrekar Pratiksha P. , Kang Mingu , Park Inkyu , Kim Bumjoo , Yang Daejong
Published: January 2023

Surface-enhanced Raman spectroscopy (SERS) has been transformed into a useful analytical technique with significant advantages in relation to sensitive and low-concentration chemical analyses. However, SERS substrates are expensive and the analyte sample preparation is complicated; hence, it is only used in limited areas. We have fabricated a tattoo paper-based SERS substrate by using non-complicated inkjet printing. The sensitivity of the SERS substrate was increased by removing the carbon residues via exposure to ultraviolet light without damaging the substrate. Thus, low concentrations of pesticides (up to 1 μM thiram) were measured. The SERS substrate was attached to the curved surface of an apple to demonstrate its advantages, such as the flexibility and easy attachability of tattoo paper, and its feasibility was verified by measuring 1 μM thiram on the apple’s surface. Due to its economic cost, simple usage, and rapid measurement, it will be helpful for the identification of both agricultural adulterants and food adulterants and for water-based pollutant detection. It will also possibly be helpful for medical purposes related to human body surfaces in the future.
Expert Consensus on Morphofunctional Assessment in Disease-Related Malnutrition. Grade Review and Delphi Study
Authors: García-Almeida José Manuel , García-García Cristina , Ballesteros-Pomar María D. , Olveira Gabriel , Lopez-Gomez Juan J. , Bellido Virginia , Bretón Lesmes Irene , Burgos Rosa , Sanz-Paris Alejandro , Matia-Martin Pilar , Botella Romero Francisco , Ocon Breton Julia , Zugasti Murillo Ana , Bellido Diego
Published: January 2023

Disease-related malnutrition (DRM) affects approximately a third of hospitalized patients and is associated with an increased risk of morbimortality. However, DRM is often underdiagnosed and undertreated. Our aim is to evaluate the prognostic value of morphofunctional tools and tests for nutritional assessment in clinical practice. A systematic literature review was conducted to identify studies relating to the morphofunctional assessment of nutritional status and mortality or complications. Evidence was evaluated using the GRADE (Grading of Recommendations, Assessment, Development, and Evaluations) method. Twelve GRADE recommendations were made and divided into seven topics: food intake and nutrient assimilation, anthropometry, biochemical analysis, hand grip strength, phase angle, muscle imaging, and functional status and quality of life. From these recommendations, 37 statements were developed and scored in a two-survey Delphi method by 183 experts. A consensus was reached on accepting 26/37 statements. Surveys had high internal consistency and high inter-rater reliability. In conclusion, evidence-based recommendations were made on the prognostic value of morphofunctional assessment tools and tests to assess malnutrition, most of which were found to be feasible in routine clinical practice, according to expert opinions.
Standardized Ethanol Extract of Cassia mimosoides var. nomame Makino Ameliorates Obesity via Regulation of Adipogenesis and Lipogenesis in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice
Authors: Heo So-Won , Chung Kyung-Sook , Yoon Young-Seo , Kim Soo-Yeon , Ahn Hye-Shin , Shin Yu-Kyong , Lee Sun-Hee , Lee Kyung-Tae
Published: January 2023

Obesity is a major cause of conditions such as type 2 diabetes and non-alcoholic fatty liver disease, posing a threat to public health worldwide. Here, we analyzed the anti-obesity effects of a standardized ethanol extract of Cassia mimosoides var. nomame Makino (EECM) in vitro and in vivo. Treatment of 3T3-L1 adipocytes with EECM suppressed adipogenesis and lipogenesis via the AMP-activated protein kinase pathway by downregulating the expression levels of CCAAT/enhancer-binding protein-alpha, peroxisome proliferator-activated receptor (PPAR)-γ, sterol regulatory element-binding protein-1, and fatty acid synthase and upregulating the acetyl-CoA carboxylase. EECM inhibited mitotic clonal expansion during early adipocyte differentiation. Oral administration of EECM for 10 weeks significantly alleviated body weight gain and body fat accumulation in high-fat diet (HFD)-fed mice. EECM mitigated adipogenesis and lipid accumulation in white adipose and liver tissues of HFD-induced obese mice. It regulated the levels of adipogenic hormones including insulin, leptin, and adipokine in the blood plasma. In brown adipose tissue, EECM induced the expression of thermogenic factors such as uncoupling protein-1, PPAR-α, PPARγ co-activator-1α, sirtuin 1, and cytochrome c oxidase IV. EECM restored the gut microbiome composition at the phylum level and alleviated dysbiosis. Therefore, EECM may be used as a promising therapeutic agent for the prevention of obesity.
The Potential of Condiments, Seasonings, and Bouillon Cubes to Deliver Essential Micronutrients in Asia: Scenario Analyses of Iodine and Iron Fortification
Authors: Eilander Ans , Verbakel Marieke R. , Dötsch-Klerk Mariska
Published: January 2023

Micronutrient deficiencies are still highly prevalent in Asia. Fortification of cooking aids, such as condiments (fish and soy sauces), seasonings, and bouillon cubes, may be an additional strategy to improve micronutrient intake. The current study evaluated the potential impact of iodine and iron fortification of cooking aids on micronutrient intake in Asian countries. A systematic literature search was performed to collect consumption data from different countries in Asia. Data from 18 studies in nine Asian countries were included. Scenario analyses were performed using different fortification levels based on regulations and literature. Mean intake of cooking aids ranged from 3.2–15.9 g/day for condiments and 0.4–11.7 g/day for seasonings and bouillon cubes. When replacing salt with iodized salt (30 µg of iodine/g of salt), iodine intake would increase by 13–119 µg/day for soy and fish sauces (9–80% of the Nutrient Reference Value (NRV)), and 5–83 µg/day for bouillon cubes and seasonings (4–56% of the NRV). Fortification with iron 0.5 mg/g food product for condiments or 1 mg/g food product for bouillon cubes and seasonings improved iron intake for soy and fish sauces by 1.6–8.0 mg/day (11–57% of the NRV), and for bouillon cubes and seasonings by 0.4–5.6 mg/day (3–40% of the NRV). These results indicate that, depending on the consumption pattern, fortification of cooking aids can be a suitable strategy to increase intake of micronutrients.
LncRNA SNHG6 Upregulates KPNA5 to Overcome Gemcitabine Resistance in Pancreatic Cancer via Sponging miR-944
Authors: Gao Ge , Li Xin , Wu Hui , Huang Ling-li , Lin Yu-xin , Huo Zhi , Xiang Zhong-yuan , Zhou Xiao
Published: January 2023

Gemcitabine (GEM) is the gold-standard therapeutic regimen for patients with pancreatic cancer (PC); however, patients may receive limited benefits due to the drug resistance of GEM. LncRNA SNHG6 is reported to play key roles in drug resistance, but its role and molecular mechanism in PC remain incompletely understood. We found that LncRNA SNHG6 is drastically downregulated in GEM-resistant PC and is positively correlated with the survival of PC patients. With the help of bioinformatic analysis and molecular approaches, we show that LncRNA SNHG6 can sponge miR-944, therefore causing the upregulation of the target gene KPNA5. In vitro experiments showed that LncRNA SNHG6 and KPNA5 suppress PC cell proliferation and colony formation. The Upregulation of LncRNA SNHG6 and KPNA5 increases the response of GEM-resistant PANC-1 cells to GEM. We also show that the expression of KPNA5 is higher in patients without GEM resistance than in those who developed GEM resistance. In summary, our findings indicate that the LncRNA SNHG6/miR944/KPNA5 axis plays a pivotal role in overcoming GEM resistance, and targeting this axis may contribute to an increasing of the benefits of PC patients from GEM treatment.
Determination of Tyrosine Kinase Inhibitors via Capillary Electrophoresis with Tandem Mass Spectrometry and Online Stacking Preconcentration
Authors: Petr Jan
Published: January 2023

Capillary electrophoresis connected with tandem mass spectrometry was employed for the development of a method for determination of various tyrosine kinase inhibitors in plasma samples. A stacking online preconcentration with a 120 cm-long capillary was used for the determination of bosutinib, dasatinib, canertinib, and erlotinib at physiologically relevant concentrations. The optimization included both capillary electrophoresis and mass spectrometry steps. Under optimal conditions, 50 mM formic acid pH 2.5, an injection time of 120 s, and an optimized mass spectrometry set-up (as sheath liquid composition 75:24.9:0.1 (v/v) methanol, water, formic acid, and appropriate conditions for ion transitions), LODs in a range of 3.9–23.0 nmol·L−1 were observed. The method was validated in terms of linearity, limit of detection, limit of quantification, repeatability of migration times and peak area, and recovery using plasma as a matrix for analytes. The results showed that this method has great promise for use in many analytical tasks, e.g., therapeutic drug monitoring.
Preparation and In Vitro Evaluation of a Gadolinium-Containing Vitamin E TPGS Micelle as a Potential Contrast Agent for MR Imaging
Authors: Gai Yongkang , Li Yuying , Wu Shuangping , Xu Ling , Lu Yao , Lan Xiaoli , Xiang Guangya , Ma Xiang
Published: January 2023

The application of many currently evaluated macromolecular contrast agents for magnetic resonance imaging (MRI) has been limited because of their bio-incompatibility and toxicity. The aim of this study is to synthesize and characterize a new micelle-based TPGS gadolinium chelate as a biocompatible MRI contrast agent for prolonged blood circulation time and good tumor imaging contrast. The TPGS-gadolinium conjugate was prepared through the conjugation between TPGS-SA and bifunctional L-NETA-Gd chelate. The conjugate was characterized with regard to molecular weight, critical micellar concentration and particle sizes, cellular uptake, and in vitro cell MRI. Distributions of the MRI contrast agent in various organs were determined via intravenous injection of the agent into mice bearing xenograft tumors. The successfully prepared TPGS-L-NETA-Gd micelle exhibited improved cellular uptake in HepG2 cells and xenografts and high in vivo safety. Distributions of TPGS-L-NETA-Gd in mice showed enhanced cellular uptake up to 2 h after the contrast agent injection. Its in vitro and in vivo properties make it a favorable macromolecular MRI contrast agent for future in vivo imaging.
Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from Lactobacillus acidophilus-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities
Authors: Siddiqui Arif Jamal , Patel Mitesh , Adnan Mohd , Jahan Sadaf , Saxena Juhi , Alshahrani Mohammed Merae , Abdelgadir Abdelmushin , Bardakci Fevzi , Sachidanandan Manojkumar , Badraoui Riadh , Snoussi Mejdi , Ouhtit Allal
Published: January 2023

The proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin’s commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) extracted from Lactobacillus acidophilus (L. acidophilus), which may have the potential to increase the bioactivity of bacteriocins and overcome the hurdles. It was found that extracted and purified Bac10307 had a broad range of stability for both temperature (20–100 °C) and pH (3–12). Further, based on Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS–PAGE) analysis, its molecular weight was estimated to be 4.2 kDa. The synthesized Bac10307-AgNPs showed a peak of surface plasmon resonance at 430 nm λmax. Fourier transform infrared (FTIR) confirmed the presence of biological moieties, and transmission electron microscopy (TEM) coupled with Energy dispersive X-Ray (EDX) confirmed that AgNPs were spherical and irregularly shaped, with a size range of 9–20 nm. As a result, the Bac10307-AgNPs displayed very strong antibacterial activity with MIC values as low as 8 μg/mL for Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), when compared to Bac10307 alone. In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC50 = 135.63 μg/mL), more than Bac10307 alone (IC50 = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). Furthermore, a protein–protein molecular docking simulation study of bacteriocins with target proteins of different biological functions was also carried out in order to ascertain the interactions between bacteriocins and target proteins.
Polymer-Antimicrobial Peptide Constructs with Tailored Drug-Release Behavior
Authors: Pola Robert , Vícha Matěj , Trousil Jiří , Grosmanová Eliška , Pechar Michal , Rumlerová Anna , Studenovský Martin , Kučerová Emilie , Ulbrich Pavel , Vokatá Barbora , Etrych Tomáš
Published: January 2023

Microbial resistance is one of the main problems of modern medicine. Recently, antimicrobial peptides have been recognized as a novel approach to overcome the microbial resistance issue, nevertheless, their low stability, toxicity, and potential immunogenic response in biological systems have limited their clinical application. Herein, we present the design, synthesis, and preliminary biological evaluation of polymer-antibacterial peptide constructs. The antimicrobial GKWMKLLKKILK-NH2 oligopeptide (PEP) derived from halictine, honey bee venom, was bound to a polymer carrier via various biodegradable spacers employing the pH-sensitive or enzymatically-driven release and reactivation of the PEP’s antimicrobial activity. The antibacterial properties of the polymer-PEP constructs were assessed by a determination of the minimum inhibitory concentrations, followed by fluorescence and transmission electron microscopy. The PEP exerted antibacterial activity against both, gram-positive and negative bacteria, via disruption of the bacterial cell wall mechanism. Importantly, PEP partly retained its antibacterial efficacy against Staphylococcus epidermidis, Escherichia coli, and Acinetobacter baumanii even though it was bound to the polymer carrier. Indeed, to observe antibacterial activity similar to the free PEP, the peptide has to be released from the polymer carrier in response to a pH decrease. Enzymatically-driven release and reactivation of the PEP antimicrobial activity were recognized as less effective when compared to the pH-sensitive release of PEP.
The Effect of Alginate Concentration on Crystallinity, Morphology, and Thermal Stability Properties of Hydroxyapatite/Alginate Composite
Authors: Wulandari Wulandari , Islami Dini Muthiah , Wellia Diana Vanda , Emriadi Emriadi , Sisca Vivi , Jamarun Novesar
Published: January 2023

Hydroxyapatite (HAp) has been used for various applications such as orthopedics, drug delivery material, and bone tissue engineering. It is well known that HAp has a good biocompatibility and osteoconductivity, so HAp can be used in biomedical applications. Hydroxyapatite can be combined with other materials, in particular polymer, to expand its range of applications. In this study, the polymer that will be used as a support for the HAp composite is alginate (Alg). The HAp/Alg composite has been synthesized by the precipitation method. The XRD results show that the crystal system of HAp was hexagonal. The spheric-like shaped particles can be observed from SEM images, and particle size distribution spread from 400 to 1100 nm. The EDS spectrum exhibited the peak of Ca, C, P, and O elements, indicating that alginate had interacted with hydroxyapatite in the synthesized composite. The as-fabricated composite showed not only good crystallinity but also high thermal resistance. Thermogravimetric-differential thermal analysis (TGA-DTA) revealed that the HAp/Alg composites have a constant weight at 750 °C, so it might be applied in advanced applications such as bioimaging, drug carrier, and other cancer treatments.
Mechanical Properties and Durability of Geopolymer Recycled Aggregate Concrete: A Review
Authors: Zhang Peng , Sun Xiaoyao , Wang Fei , Wang Juan
Published: January 2023

Geopolymer recycled aggregate concrete (GPRAC) is a new type of green material with broad application prospects by replacing ordinary Portland cement with geopolymer and natural aggregates with recycled aggregates. This paper summarizes the research about the mechanical properties, durability, and microscopic aspects of GPRAC. The reviewed contents include compressive strength, elastic modulus, flexural strength, splitting tensile strength, freeze–thaw resistance, abrasion resistance, sulfate corrosion resistance, and chloride penetration resistance. It is found that GPRAC can be made to work better by changing the curing temperature, using different precursor materials, adding fibers and nanoparticles, and setting optimal mix ratios. Among them, using multiple precursor materials in synergy tended to show better performance compared to a single precursor material. In addition, using modified recycled aggregates, the porosity and water absorption decreased by 18.97% and 25.33%, respectively, and the apparent density was similar to that of natural aggregates. The current results show that the performance of GPRAC can meet engineering requirements. In addition, compared with traditional concrete, the use of GPRAC can effectively reduce carbon emissions, energy loss, and environmental pollution, which is in line with the concept of green and low-carbon development in modern society. In general, GPRAC has good prospects and development space. This paper reviews the effects of factors such as recycled aggregate admixture and curing temperature on the performance of GPRAC, which helps to optimize the ratio design and curing conditions, as well as provide guidance for the application of recycled aggregate in geopolymer concrete, and also supply theoretical support for the subsequent application of GPRAC in practical engineering.