bluuebunny/crossref_metadata_2025 huggingface dataset:
An Open-Source Benchmark of IEEE Test Cases for Easily Testing a New Approach for Steady State Calculations in Power Systems
Authors: Feghali Joy El , Cossart Quentin , Bureau Gautier , Letellier Baptiste , Menezes Ian , Rosiere Florentine , Chiaramello Marco
Published: March 2024

Power systems modeling and simulation are essential to conduct studies on the electrical transmission system and ensure its security. For this purpose, RTE, the French Transmission System Operator (TSO), is developing Dynaωo, a hybrid Modelica/C++ open-source suite of simulation tools for power systems. Most power systems models for Dynaωo are developed in the Modelica language using the Dynaωo Modelica library. This paper presents a full Modelica standard electrical power system benchmark implemented using the Dynaωo library. The IEEE 14-bus system benchmark is modeled here for steady-state calculation, with an approach that replaces the static load flow. Two test cases are simulated using the OpenModelica environment showing differences in the final steady-state result. We show flexibility in modeling with this library where different system behaviors can be observed and where models with different levels of details can be replaced depending on the application: steady-state calculation, long-term stability, or short-term stability.
Switching and Averaging Models of a Bidirectional, Half-Bridge Based DC-DC converter with Load Distribution
Authors: Reindl Andrea , Lang Andreas , Niemetz Michael , Meier Hans
Published: March 2024

Batteries are used in numerous applications such as mobile devices, electric vehicles, home storage systems and islanded microgrids. Bidirectional DC-DC converters are vital for the integration of batteries, for the power conversion during (dis)charge and the battery management. Modeling of these is helpful, especially for the design of larger, more complex systems consisting of multiple DC-DC converters in parallel. Due to the high switching frequencies, the simulation of DC-DC converters is associated with increased computational time and effort. In this paper, three models of different complexity and accuracy are proposed for a bidirectional DC-DC converter consisting of two phase-shifted half-bridges. Two switching models, which differ mainly in the way the mosfets are driven, account for the individual switching operations and exhibit high accuracy. An averaging model replaces the switching elements with current and voltage sources providing the mean values. It is particularly suitable for multiple components and longer simulation durations. The dynamic behavior of the models is analyzed using the step responses of the load current. For validation, these are compared with the theoretical transfer function. The three models are analyzed comparatively in terms of computational time and effort. The calculation time of the averaging model has been reduced by two thirds compared to the strictly complementary switching model and by 96% relative to the model with diode emulation mode. The averaging model requires only one third of the computation time of the complementary switching model and only 3.5% of that of the model with diode emulation. Recommendations for the use of the models are given and a possible use case is shown. Two parallel connected DC-DC converters with load current sharing between them are simulated using the averaging model.
Gallbladder microbial species and host bile acids biosynthesis linked to cholesterol gallstone comparing to pigment individuals
Authors: Zhang Xinpeng , Hu Junqing , Li Yi , Tang Jichao , Yang Kaijin , Zhong Ayan , Liu Yanjun , Zhang Tongtong
Published: March 2024

Gallstones are crystalline deposits in the gallbladder that are traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota and host metabolism variances among the different types of gallstones remain largely unclear. Here, the bile and gallstone microbial species spectra of 29 subjects with gallstone disease (GSD, 24 cholesterol and 5 pigment) were revealed by type IIB restriction site-associated DNA microbiome sequencing (2bRAD-M). Among them (21 subjects: 18 cholesterol and 3 pigment), plasma samples were subjected to liquid chromatography–mass spectrometry (LC-MS) untargeted metabolomics. The microbiome yielded 896 species comprising 882 bacteria, 13 fungi, and 1 archaeon. Microbial profiling revealed significant enrichment of Cutibacterium acnes and Microbacterium sp005774735 in gallstone and Agrobacterium pusense and Enterovirga sp013044135 in the bile of cholesterol GSD subjects. The metabolome revealed 2296 metabolites, in which malvidin 3-(6’’-malonylglucoside), 2-Methylpropyl glucosinolate, and ergothioneine were markedly enriched in cholesterol GSD subjects. Metabolite set enrichment analysis (MSEA) demonstrated enriched bile acids biosynthesis in individuals with cholesterol GSD. Overall, the multi-omics analysis revealed that microbiota and host metabolism interaction perturbations differ depending on the disease type. Perturbed gallstone type-related microbiota may contribute to unbalanced bile acids metabolism in the gallbladder and host, representing a potential early diagnostic marker and therapeutic target for GSD.
Developing behavioral models of citizens for adapting to and mitigating climate change: a study on four prefectures in Japan
Authors: Aoki Eri , Shirai Nobuo , Baba Kenshi , Masuhara Naoki , Taniguchi Makoto
Published: March 2024

Individual behaviors of citizens are important for implementing the necessary measures for adapting to climate change. However, studies on the adaptive behaviors of ordinary citizens are limited. Therefore, in this study, we developed behavioral models to understand individual behaviors for adaption to and mitigation of climate change. We compared four behavior groups, namely, G1, individual mitigation; G2, individual adaptive; G3, long-term adaptive; and G4, solar-energy system installation behaviors. Following a questionnaire survey for the four behavioral groups, behavioral models were developed using structural equation modeling, which considered psychological factors along with selected attitudes, perceived effectiveness, norms, benefits, practicality, and intention. Furthermore, we considered the recognition and attribution of local climate change as key factors for adaptation behaviors toward local climate change. The behaviors of citizens in four prefectures in Japan, namely, Nagano, Tokyo, Saitama, and Kanagawa were considered. Among them, Nagano Prefecture, wherein the implementation of adaptation measures was at a low level while that of mitigation measures was at a high level, showed a differing trend. In the behavioral models, the recognition of local climate change directly affected the behavior of citizens in the individual adaptive behavior group. In both the individual adaptive and long-term adaptive behavior groups, the impact of benefits was substantial, with significant differences across local areas. Thus, effective adaptation strategies might involve tangible and familiar examples demonstrating the consequences of behavior, thus enhancing citizens' behavior evaluation perception and fostering its acceptance as a desirable behavior. Tailoring adaptation strategies to specific local contexts might also be necessity. To encourage citizens to adapt to climate change and its impacts, we propose two-way efforts, instead of providing one-way information, by supporting citizens to think about self-help and mutual help effectively. Our study can serve as a reference for future studies focusing on citizen behavior about climate change mitigation and enhance their adaptation for locally implemented strategies.
Gender-specific genetic and epigenetic signatures in cardiovascular disease
Authors: Bridges Justin , Ramirez-Guerrero Jose A. , Rosa-Garrido Manuel
Published: March 2024

Cardiac sex differences represent a pertinent focus in pursuit of the long-awaited goal of personalized medicine. Despite evident disparities in the onset and progression of cardiac pathology between sexes, historical oversight has led to the neglect of gender-specific considerations in the treatment of patients. This oversight is attributed to a predominant focus on male samples and a lack of sex-based segregation in patient studies. Recognizing these sex differences is not only relevant to the treatment of cisgender individuals; it also holds paramount importance in addressing the healthcare needs of transgender patients, a demographic that is increasingly prominent in contemporary society. In response to these challenges, various agencies, including the National Institutes of Health, have actively directed their efforts toward advancing our comprehension of this phenomenon. Epigenetics has proven to play a crucial role in understanding sex differences in both healthy and disease states within the heart. This review presents a comprehensive overview of the physiological distinctions between males and females during the development of various cardiac pathologies, specifically focusing on unraveling the genetic and epigenetic mechanisms at play. Current findings related to distinct sex-chromosome compositions, the emergence of gender-biased genetic variations, and variations in hormonal profiles between sexes are highlighted. Additionally, the roles of DNA methylation, histone marks, and chromatin structure in mediating pathological sex differences are explored. To inspire further investigation into this crucial subject, we have conducted global analyses of various epigenetic features, leveraging data previously generated by the ENCODE project.
The thrombotic risk in Cushing’s syndrome—questions, answers, and the algorithm to consider in its assessment: part I—thrombotic risk not related to surgery
Authors: Bryk-Wiązania Agata Hanna , Minasyan Mari , Świątkowska-Stodulska Renata , Undas Anetta , Hubalewska-Dydejczyk Alicja , Webb Susan M. , Valassi Elena , Gilis-Januszewska Aleksandra
Published: March 2024

Introduction

Recently, it has been reported that there is a great diversity in strategies used for thromboprophylaxis in patients with Cushing’s syndrome (CS). An aim of this review was to discuss these practices in light of the existing data on the thrombotic risk in patients with CS and guidelines for medically ill patients.

Methods

The four relevant topics and questions on thrombotic risk in CS were identified. The current guidelines on prevention and diagnosis of venous thromboembolism (VTE) were reviewed for the answers. An algorithm to consider in the assessment of the thrombotic risk in patients with CS was proposed.

Results

To address both generic and CS-specific risk factors for VTE, the algorithm includes the stepwise approach consisting of Padua Score, urine free cortisol, and CS-VTE score, with no indication for routine thrombophilia testing in the prediction of an index VTE episode. Having confirmed VTE, selected patients require thrombophilia testing to aid the duration of anticoagulant treatment. The separate part of the algorithm is devoted to patients with ectopic adrenocorticotropic hormone syndrome in whom exclusion of VTE precedes introducing routine thromboprophylaxis to prevent VTE. The cancer-related VTE also prompts thromboprophylaxis, with the possible vessel invasion. The algorithm presents a unifactorial and multifactorial approach to exclude high-bleeding risks and safely introduce thromboprophylaxis with low-molecular-weight heparin.

Our article is the first to present an algorithm to consider in the thrombotic risk assessment among patients with Cushing’s syndrome as a starting point for a broader discussion in the environment. A plethora of factors affect the VTE risk in patients with CS, but no studies have conclusively evaluated the best thromboprophylaxis strategy so far. Future studies are needed to set standards of care.
Sirtuin 1 serum concentration in healthy children - dependence on sex, age, stage of puberty, body weight and diet
Authors: Fedorczak Anna , Lewiński Andrzej , Stawerska Renata
Published: March 2024

Introduction

Sirtuin 1 (SIRT1) is known to be involved in sensing cellular energy levels and regulating energy metabolism. This study aimed to evaluate fasting serum SIRT1 levels in healthy children, and to analyse the influence of age, sex, puberty, body weight, height, and diet on its concentration.

Methods

47 healthy children aged 4-14 with weight and height within normal range and no chronic disease were included into the study. Fasting serum SIRT1 concentrations were estimated by Enzyme Linked Immunosorbent Assay (ELISA).

Results

Results showed that serum SIRT1 concentrations in healthy children did not differ with respect to sex, age, height, weight and puberty. Whereas, it appeared that a higher frequency of fruits, vegetables and dairy products consumption was associated with an increase in serum SIRT1 levels.

Discussion

Studying SIRT1 in the context of children’s health may have implications for a broader understanding of growth processes, pubertal development, metabolic disorders and nutrition.
Causal relationship between genetically predicted type 2 diabetes mellitus and male infertility
Authors: Fan Cuihua , Zhang Jiandong , Qiu Dongbiao
Published: March 2024

Background

Diabetes mellitus (DM) stands as the most prevalent endocrine abnormality affecting the physiological systems and organs and impairing the male reproductive functions. Type 2 Diabetes Mellitus (T2DM), accounting for about 90-95% of DM, is closely associated with male infertility. However, the magnitude of the causal relationships between T2DM and male infertility remains unclear. The current investigation was to explore the causal relationship between T2DM and male infertility utilizing the Mendelian Randomization (MR) analysis.

Methods

A two-sample MR (2SMR) analysis was conducted to investigate the causal relationship between T2DM and male infertility in the European population from the genome-wide association study (GWAS) summary data that was publicly accessible. GWAS for T2DM and male infertility were extracted from the IEU Open GWAS Project database, with the resulting data encompassing 680 cases and 72,799 controls as the outcome data. Five MR methods were employed for the 2SMR analyses, namely the MR-Egger, weighted median estimation (WME), weighted mode (WM), inverse-variance weighted (IVW), and simple mode. The primary analytical technique utilized in this study was the IVW method, and a multivariate MR analysis was executed to examine the potential mediating influences of T2DM on male infertility.

Results

Following were the odds ratios (ORs) and associated 95% CIs derived from IVW (fixed effects), MR-Egger, WM, WME, and simple mode approaches: 0.824 (95% CI 0.703-0.966), 0.726 (95% CI 0.527-1.001), 0.827 (95% CI 0.596-1.150), 0.841 (95% CI 0.654-1.082), and 0.875 (95% CI 0.544-1.405), respectively. The outcomes of the heterogeneity tests were P=0.378 and P=0.384, respectively, implying no heterogeneity. Egger-intercept outcomes were P=0.374, highlighting the absence of pleiotropy. The stability of the results was affirmed through the leave-one-out analysis. Notably, all F-values surpassed 10, indicating the absence of weak bias attributed to instrument variables(IVs).

Conclusions

This research furnishes evidence supporting a causal association between T2DM and male infertility. These insights offer a foundation for future investigations aiming to establish the association between genetically predicted T2DM and male infertility. These outcomes suggest the significance of active monitoring and proactive measures for preventing infertility in male individuals with T2DM. Furthermore, careful consideration is required for individuals of reproductive age to prevent and treat T2DM.
A lactate metabolism-related signature predicting patient prognosis and immune microenvironment in ovarian cancer
Authors: Zhu Linhua , Lin Zhuoqun , Wang Kai , Gu Jiaxin , Chen Xiaojing , Chen Ruizhe , Wang Lingfang , Cheng Xiaodong
Published: March 2024

Introduction

Ovarian cancer (OV) is a highly lethal gynecological malignancy with a poor prognosis. Lactate metabolism is crucial for tumor cell survival, proliferation, and immune evasion. Our study aims to investigate the role of lactate metabolism-related genes (LMRGs) in OV and their potential as biomarkers for prognosis, immune microenvironment, and immunotherapy response.

Methods

Ovarian samples were collected from the TCGA cohort. And 12 lactate-related pathways were identified from the MsigDB database. Differentially expressed genes within these pathways were designated as LMRGs, which undergo unsupervised clustering to identify distinct clusters based on LMRGs. Subsequently, we assessed survival outcomes, immune cell infiltration levels, Hallmaker pathway activation patterns, and chemotaxis among different subtypes. After conducting additional unsupervised clustering based on differentially expressed genes (DEGs), significant differences in the expression of LMRGs between the two clusters were observed. The differentially expressed genes were subjected to subsequent functional enrichment analysis. Furthermore, we construct a model incorporating LMRGs. Subsequently, the lactate score for each tumor sample was calculated based on this model, facilitating the classification of samples into high and low groups according to their respective lactate scores. Distinct groups examined disparities in survival prognosis, copy number variation (CNV), single nucleotide variation (SNV), and immune infiltration. The lactate score served as a quantitative measure of OV's lactate metabolism pattern and an independent prognostic factor.

Results

This study investigated the potential role of LMRGs in tumor microenvironment diversity and prognosis in OV, suggesting that LMRGs play a crucial role in OV progression and the tumor microenvironment, thus serving as novel indicators for prognosis, immune microenvironment status, and response to immunotherapy.
Mannose enhances intestinal immune barrier function and dextran sulfate sodium salt-induced colitis in mice by regulating intestinal microbiota
Authors: Yang Yi , Ma Qiming , Wang Qingyu , Zhao Lifeng , Liu Hengshan , Chen Yanjun
Published: March 2024

Background

Inflammatory bowel disease (IBD) greatly affects human quality of life. Mannose has been reported to be used to treat IBD, but the mechanism is currently unknown.

Methods

C57/BL mice were used as research subjects, and the mouse acute colitis model was induced using dextran sulfate sodium salt (DSS). After oral administration of mannose, the body weights and disease activity index (DAI) scores of the mice were observed. The colon lengths, histopathological sections, fecal content microbial sequencing, colon epithelial inflammatory genes, and tight junction protein Occludin-1 expression levels were measured. We further used the feces of mice that had been orally administered mannose to perform fecal bacterial transplantation on the mice with DSS-induced colitis and detected the colitis-related indicators.

Results

Oral administration of mannose increased body weights and colon lengths and reduced DAI scores in mice with DSS-induced colitis. In addition, it reduced the expression of colon inflammatory genes and the levels of serum inflammatory factors (TNF-α, IL-6, and IL-1β), further enhancing the expression level of the colonic Occludin-1 protein and alleviating the toxic response of DSS to the intestinal epithelium of the mice. In addition, gut microbial sequencing revealed that mannose increased the abundance and diversity of intestinal flora. Additionally, after using the feces of the mannose-treated mice to perform fecal bacterial transplantation on the mice with DSS-induced colitis, they showed the same phenotype as the mannose-treated mice, and both of them alleviated the intestinal toxic reaction induced by the DSS. It also reduced the expression of intestinal inflammatory genes (TNF-α, IL-6, and IL-1β) and enhanced the expression level of the colonic Occludin-1 protein.

Conclusion

Mannose can treat DSS-induced colitis in mice, possibly by regulating intestinal microorganisms to enhance the intestinal immune barrier function and reduce the intestinal inflammatory response.
Taxonomic and functional-trait metrics track recovery of demersal fish and shrimp communities following system collapse
Authors: Aune Magnus , Ramasco Virginie , Wells Nadine , Warren Margaret , Cyr Frédéric , Pedersen Eric J. , Koen-Alonso Mariano , Eriksen Elena , Primicerio Raul , Lindstrøm Ulf , Renaud Paul E.
Published: March 2024

Monitoring programs that integrate both structural and functional ecosystem components play integral roles in ecosystem management and conservation planning. In the early 1990’s, the marine ecosystem of the waters surrounding Newfoundland and Labrador (NL) underwent a regime shift. Several demersal and pelagic fish stocks collapsed simultaneously, and this had significant ecological and socioeconomic consequences. As this regime shift impacted numerous commercial and non-commercial species, assessments based on individual species would be insufficient. We explored a variety of metrics that capture different facets of diversity across multiple species to provide a more robust ecosystem assessment. These were species richness, evenness, community-weighted means of maximum body length and trophic level (i.e., the mean maximum body size or trophic level of the species present in a community), and functional dispersion (FDis). The objectives of this study were 1) to assess trends in community structure of the NL demersal community during the post-collapse period (1995-2018), 2) explore how the various community-level metrics differ or are redundant, and 3) investigate how these metrics are associated with important covariates. Several metrics were redundant and displayed strongly positive, temporal trends being consistent with expectations for a recovery encompassing the entire demersal community. In particular, unweighted community-weighted means of body length and trophic level displayed nearly equal temporal patterns, showing increasing trends throughout the study period which were most prominent in the northern study area and within a limited depth range at the upper shelf break. Corresponding biomass-weighted metrics were also correlated with each other but only showed similar increasing tendencies after the first decade. In contrast, species richness did not show any temporal increase. Evenness and biomass-weighted FDis showed similar temporal patterns, decreasing during the first decade followed by strong increases during subsequent years, patterns that were directly linked to variation in Northern shrimp and Atlantic cod biomass. This study demonstrates how a variety of community metrics can provide insight into different aspects of the post-collapse recovery of the demersal community and help us better understand the complexity of the changes the ecosystem is undergoing.
A demographic model to forecast Dinophysis acuminata harmful algal blooms
Authors: Vieira Vasco Manuel Nobre de Carvalho da Silva , Rosa Teresa Leal , Sobrinho-Gonçalves Luís , Mateus Marcos Duarte , Mota Bernardo
Published: March 2024

Harmful algal blooms (HABs) in marine environments have significant adverse effects on public health, aquaculture and recreational activities. Surges of certain phytoplanktonic toxin-producing microalgae (mostly dinoflagellates or diatoms species) can induce Amnesic, Diarrhetic or Paralytic Shellfish Poisoning (ASP, DSP and PSP). Among HAB species, the genus Dinophysis leads to DSP in human consumers; this being the most recurrent problem in the Iberian Peninsula with the biggest economic impact on clam production and harvesting. While complete elimination of HABs is not feasible, timely implementation of appropriate measures can prevent their negative consequences. This is critical for aquaculture. Research on D. acuminata (dominant Dinophysis species in the North Atlantic) has been focused on ecophysiology and population dynamics, although with few modelling attempts. Weekly monitoring along the Portuguese coast since 2006 has revealed that D. acuminata thrives under spring/summer photosynthetically active radiation (PAR) coupled with water temperatures below 20°C, which typically coincide with the local upwelling regime. In order to advance this knowledge numerically, we developed a demographic model linking D. acuminata growth rate to PAR and sea surface temperature (SST). The 13-year (1-Jan-2006 to 31-Dec-2018) time-series of observations was closely fit by model forecasts. However, the model demonstrated limitations in issuing timely warnings of harmful proliferation of D. acuminata, failing to do so in 50% of cases, and issuing incorrect warnings in 5% of the cases. Furthermore, improving the odds of emitting timely warnings always worsened the odds of emitting false warnings, and vice-versa. To simultaneously improve both aspects, the modelling results clearly indicated the need of implementing both census/projection intervals smaller than 7 days and a laboratory detection limit below 20 cell/L. The time resolution of the census and of the model proved to be the most limiting factor that must be addressed in order to improve numerical forecasting of HABs.
Diagnostic accuracy of oral swab for detection of pulmonary tuberculosis: a systematic review and meta-analysis
Authors: Zhang Fuzhen , Wang Yilin , Zhang Xuxia , Liu Kewei , Shang Yuanyuan , Wang Wei , Liu Yuanyuan , Li Liang , Pang Yu
Published: March 2024

Objectives

Tuberculosis (TB) remains a significant concern in terms of public health, necessitating the timely and accurate diagnosis to impede its advancement. The utilization of oral swab analysis (OSA) presents a promising approach for diagnosing pulmonary TB by identifying Mycobacterium tuberculosis (MTB) within oral epithelial cells. Due to disparities in the diagnostic performance of OSA reported in the original studies, we conducted a meticulous meta-analysis to comprehensively assess the diagnostic efficacy of OSA in pulmonary TB.

Methods

We conducted a comprehensive investigation across multiple databases, namely PubMed, Cochrane Library, Embase, Web of Science, ClinicalTrials.gov, Chinese BioMedical Literature Database (CBM), China National Knowledge Infrastructure Database (CNKI), and Wanfang China Science and Technology Journal Database to identify relevant studies. Out search query utilized the following keywords: oral swab, buccal swab, tongue swab, tuberculosis, and TB. Subsequently, we employed STATA 16.0 to compute the combined sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio for both the overall and subgroup analyses.

Results

Our findings indicated that OSA has a combined sensitivity of 0.67 and specificity of 0.95 in individuals with pulmonary TB. Subgroup analysis further revealed that among adult individuals with pulmonary TB, the sensitivity and specificity of OSA were 0.73 and 0.93, respectively. In HIV-negative individuals with pulmonary TB, the sensitivity and specificity were 0.68 and 0.98, respectively. The performance of OSA in detecting pulmonary TB correlated with the bacteria load in sputum. Additionally, the sensitivity for diagnosing pulmonary TB using tongue specimens was higher (0.75, 95% CI: 0.65–0.83) compared to cheek specimens (0.52, 95% CI: 0.34–0.70), while both types of specimens demonstrated high specificity.

Conclusions

To conclude, oral swabs serve as a promising alternative for diagnosing pulmonary TB, especially in adult patients. In addition, tongue swabs yield better sensitivity than cheek swabs to identify pulmonary TB patients.

Systematic review registration

identifier: CRD42023421357.
Metagenomic sequencing identified microbial species in the rumen and cecum microbiome responsible for niacin treatment and related to intramuscular fat content in finishing cattle
Authors: Yang Zhuqing , Chen Xiao , Yu Mingjin , Jing Ruixue , Bao Linbin , Zhao Xianghui , Pan Ke , Chao Bihui , Qu Mingren
Published: March 2024

Introduction

Niacin is one of the essential vitamins for mammals. It plays important roles in maintaining rumen microecological homeostasis. Our previous study indicated that dietary niacin significantly elevated intramuscular fat content (IMF) in castrated finishing steers. Whether niacin affects fat deposition by regulating the microbial composition and functional capacities of gastrointestinal microbiome has been unknown yet.

Methods

In this study, 16 castrated Xiangzhong Black cattle were randomly assigned into either control group fed with a basal concentrate diet (n = 8) or niacin group fed with a basal concentrate diet added 1000 mg/kg niacin (n = 8). Seven rumen samples and five cecum content samples were randomly collected from each of control and niacin groups for metagenomic sequencing analysis.

Results

A total of 2,981,786 non-redundant microbial genes were obtained from all tested samples. Based on this, the phylogenetic compositions of the rumen and cecum microbiome were characterized. We found that bacteria dominated the rumen and cecum microbiome. Prevotella ruminicola and Ruminococcus flavefaciens were the most abundant bacterial species in the rumen microbiome, while Clostridiales bacterium and Eubacterium rectale were predominant bacterial species in the cecum microbiome. Rumen microbiome had significantly higher abundances of GHs, GTs, and PLs, while cecum microbiome was enriched by CBMs and AAs. We found a significant effect of dietary niacin on rumen microbiome, but not on cecum microbiome. Dietary niacin up-regulated the abundances of bacterial species producing lactic acid and butyrate, fermenting lactic acid, and participating in lipid hydrolysis, and degradation and assimilation of nitrogen-containing compounds, but down-regulated the abundances of several pathogens and bacterial species involved in the metabolism of proteins and peptides, and methane emissions. From the correlation analysis, we suggested that niacin improved nutrient digestion and absorption, but reduced energy loss, and Valine, leucine and isoleucine degradation of rumen microbiome, which resulted in the increased host IMF.

Conclusion

The results suggested that dietary manipulation, such as the supplementation of niacin, should be regarded as the effective and convenient way to improve IMF of castrated finishing steers by regulating rumen microbiome.
Cognitive impairment in Chinese traumatic brain injury patients: from challenge to future perspectives
Authors: Liu Tao , Yu Shaohui , Liu Mingqi , Zhao Zhihao , Yuan Jiangyuan , Sha Zhuang , Liu Xuanhui , Qian Yu , Nie Meng , Jiang Rongcai
Published: March 2024

Traumatic Brain Injury (TBI) is a prevalent form of neurological damage that may induce varying degrees of cognitive dysfunction in patients, consequently impacting their quality of life and social functioning. This article provides a mini review of the epidemiology in Chinese TBI patients and etiology of cognitive impairment. It analyzes the risk factors of cognitive impairment, discusses current management strategies for cognitive dysfunction in Chinese TBI patients, and summarizes the strengths and limitations of primary testing tools for TBI-related cognitive functions. Furthermore, the article offers a prospective analysis of future challenges and opportunities. Its objective is to contribute as a reference for the prevention and management of cognitive dysfunction in Chinese TBI patients.
Quality changes in Chinese high-quality indica rice under different storage temperatures with varying initial moisture contents
Authors: Zhu Dongyi , Wang Tingting , Liu Xiuying , Bi Jie , Zhang Wei , Zeng Xuefeng , Wang Pingping , Shu Zaixi
Published: March 2024

The planting area of high-quality indica rice varieties has been growing rapidly in China. However, the storage characteristics of these varieties remains unclear. In this research, different moisture contents (13.5, 14.5, and 15.5%) of high-quality rice (variety Xiadao No.1) were stored at different temperatures (15, 20, 25, and 30°C) for 360 d, and then evaluated for lipid metabolism, redox enzyme activities, fatty acid composition, and sensory attributes. With the prolongation of storage, rice displayed an upward trend in fatty acid value, malondialdehyde content, and cooked rice hardness and a downward trend in contents of total fat and non-starch lipid, peroxidase and catalase activities, and sensory score of cooked rice. The change trends of these quality parameters were aggravated by elevating storage temperature and moisture content. Linoleic acid content of rice generally decreased with prolonged storage. After 300 d of storage, rice with initial moisture content of 13.5% at 30°C showed a fatty acid value of higher than 30 mg KOH/100 g, while rice of other two initial moisture contents reached similar level at 25°C. After the whole storage period, only rice with initial moisture contents of 13.5 and 14.5% stored at 15°C had a sensory score of higher than 60. These results suggested that the aging process of high-quality rice can be inhibited by decreasing the storage temperature and initial moisture content. These results can provide reference for grain storage enterprises to select proper storage condition to store high-quality rice.
The imaging features of ectopic spleen: which modality is more consistent? A cases series report and literature reviews
Authors: Xiao Mingyue , Liang Jiayi , Ren Jie , Zheng Rongqin , Wu Lili
Published: March 2024

Ectopic spleen (ES) is a rare condition. It is difficult to diagnose with conventional imaging modalities. In this case series, we presented the imaging features of three misdiagnosed ES cases in our hospital and previously reported cases to compare the consistency of enhancement patterns among different imaging modalities with varied phases. Finally, 22 cases were reviewed. We determined that variable arterial phase enhancement and persistent enhancement throughout the portal and delayed phases are present in contrast-enhanced ultrasound (CEUS) imaging of the ES and found the arterial phase of CEUS had the highest consistency compared with computerized tomography (CT) and magnetic resonance imaging (MRI).
EVA1A, a novel and promising prognostic biomarker in colorectal cancer
Authors: Fan Hai-hua , Zhang Hai-jun
Published: March 2024

Purpose

The purpose of this study was to investigate the potential of EVA1A as a prognostic biomarker for Colorectal cancer (CRC).

Methods

The study utilized public databases to analyze the difference in Evala mRNA expression between CRC tumor tissues and adjacent normal tissues. Additionallymunohistochemical staining was performed on 90 paired tissue samples to detect EVA1A expression. The relationship between EVA1A and clinicopathological features was examined, and a Kaplan-Meier survival analysis was conducted. Univariate and multivariate Cox analyses were employed to identify prognostic factors affecting the overall survival (OS) of CRC patients.

Results

The analysis revealed a significant increase in Evala mRNA expression in CRC tumor cells compared to normal controls from public databases (P< 0.05). Immunohistochemical staining further confirmed a significant upregulation of EVA1A expression in CRC tissues (P< 0.05). High EVA1A expression was associated with age, pathological M stage, total tumor stage, and Carbohydrate antigen CA19-9 (CA19-9). Kaplan-Meier analysis demonstrated a significant association between high EVA1A expression and poor OS. Univariate and multivariate analysis identified EVA1A as an independent risk factor for CRC prognosis.

Conclusion

The study suggests that EVA1A is increased in CRC tumor tissues and may serve as a potential biomarker for poor prognosis in CRC.
Case report: Immunotherapy in rare high TMB pancreatic acinar carcinoma
Authors: Wu Guifu , Fang Yuting , Bi Deying , Yang Wenwei , Sun Yongkun
Published: March 2024

This case report details a patient with Pancreatic Acinar Cell Carcinoma (PACC), a rare malignancy with distinctive biological and imaging features. In the absence of standardized treatment protocols for PACC, we embarked on a diagnostic journey that led to the adoption of an innovative therapeutic regimen in our institution. A 45-year-old female patient presented with a pancreatic mass, which was histologically confirmed as PACC following a biopsy. Subsequent genomic profiling revealed a high tumor mutational burden (21.4/Mb), prompting the initiation of combined immunotherapy and targeted therapy. Notably, the patient experienced a unique adverse reaction to the immunotherapy—recurrent subcutaneous soft tissue nodules, particularly in the gluteal and lower limb regions, accompanied by pain, yet resolving spontaneously. Following six cycles of the dual therapy, radiological evaluations indicated a decrease in tumor size, leading to a successful surgical excision. Over a 20-month post-surgical follow-up, the patient showed no signs of disease recurrence. This narrative adds to the existing knowledge on PACC and highlights the potential efficacy of immunotherapy in managing this challenging condition, emphasizing the importance of close monitoring for any adverse reactions.
Evaluation of physical variables, thermal nociceptive threshold testing and pharmacokinetics during placement of transdermal buprenorphine matrix-type patch in healthy adult horses
Authors: Paranjape Vaidehi V. , Knych Heather K. , Berghaus Londa J. , Cathcart Jessica , Giancola Shyla , Craig Hannah , James Caroline , Saksena Siddharth , Reed Rachel A.
Published: March 2024

Background

Matrix type transdermal buprenorphine patches have not been investigated in horses and may provide an effective means of providing continuous pain control for extended period and eliminating venous catheterization.

Objective

Assessment of the physiological variables (heart rate, respiratory rate, body temperature) and thermal nociceptive threshold testing, and describing the pharmacokinetic profile of transdermal buprenorphine matrix-type patch (20 μg h−1 and 40 μg h−1 dosing) in healthy adult horses.

Study design

Randomised experimental study with a Latin-square design.

Methods

Six adult healthy horses received each of the three treatments with a minimum 10 day washout period. BUP0 horses did not receive a patch (control). BUP20 horses received one patch (20 μg h−1) applied on the ventral aspect of the tail base resulting in a dose of 0.03–0.04 μg kg−1 h−1. BUP40 horses received two patches placed alongside each other (40 μg h−1) on the tail base resulting in a dose of 0.07–0.09 μg kg−1 h−1. Whole blood samples (for determination of buprenorphine concentration), physiological variables and thermal threshold testing were performed before (0 h) and at 2, 4, 8, 12, 16, 24, 32, 40, 48, 56, 64, 72, and 96 h after patch application. The patches were removed 72 h following placement and were analyzed for residual buprenorphine content.

Results

Between the three groups, there was no change in physiological variables across timepoints as compared to baseline (p > 0.1). With the higher dose, there was a significant increase in thermal thresholds from baseline values from 2 h until 48 h and these values were significantly higher than the group receiving the lower patch dose for multiple timepoints up to 40 h. 40 μg h−1 patch led to consistent measurable plasma concentrations starting at 2 h up to 96 h, with the mean plasma concentrations of > 0.1 ng/ml from 4 h to 40 h.

Conclusions

20 μg h−1 and 40 μg h−1 patch doses were well tolerated by all horses. At higher dose, plasma buprenorphine concentrations were more consistently measurable and blunted thermal thresholds for 48 h vs. 32 h with 20 μg h−1 dosing as compared to control.
New targets of nascent lymphatic vessels in ocular diseases
Authors: Wu Xuhui , Ma Yunkun , Zhang Zhaochen , Hou Tingting , He Yuxi
Published: March 2024

Recent advancements in the field of endothelial markers of lymphatic vessels and lymphangiogenic factors have shed light on the association between several ocular diseases and ocular nascent lymphatic vessels. The immune privilege of corneal tissue typically limits the formation of lymphatic vessels in a healthy eye. However, vessels in the eyes can potentially undergo lymphangiogenesis and be conditionally activated. It is evident that nascent lymphatic vessels in the eyes contribute to various ocular pathologies. Conversely, lymphatic vessels are present in the corneal limbus, ciliary body, lacrimal glands, optic nerve sheaths, and extraocular muscles, while a lymphatic vasculature-like system exists in the choroid, that can potentially cause several ocular pathologies. Moreover, numerous studies indicate that many ocular diseases can influence or activate nascent lymphatic vessels, ultimately affecting patient prognosis. By understanding the mechanisms underlying the onset, development, and regression of ocular nascent lymphatic vessels, as well as exploring related research on ocular diseases, this article aims to offer novel perspectives for the treatment of such conditions.
Seed shape and size of Silene latifolia, differences between sexes, and influence of the parental genome in hybrids with Silene dioica
Authors: Marcel Hubinský , Javier Martín-Gómez José , Emilio Cervantes , Roman Hobza , Jose Luis Rodríguez Lorenzo
Published: March 2024

Introduction

Plants undergo various natural changes that dramatically modify their genomes. One is polyploidization and the second is hybridization. Both are regarded as key factors in plant evolution and result in phenotypic differences in different plant organs. In Silene, we can find both examples in nature, and this genus has a seed shape diversity that has long been recognized as a valuable source of information for infrageneric classification.

Methods

Morphometric analysis is a statistical study of shape and size and their covariations with other variables. Traditionally, seed shape description was limited to an approximate comparison with geometric figures (rounded, globular, reniform, or heart-shaped). Seed shape quantification has been based on direct measurements, such as area, perimeter, length, and width, narrowing statistical analysis. We used seed images and processed them to obtain silhouettes. We performed geometric morphometric analyses, such as similarity to geometric models and elliptic Fourier analysis, to study the hybrid offspring of S. latifolia and S. dioica.

Results

We generated synthetic tetraploids of Silene latifolia and performed controlled crosses between diploid S. latifolia and Silene dioica to analyze seed morphology. After imaging capture and post-processing, statistical analysis revealed differences in seed size, but not in shape, between S. latifolia diploids and tetraploids, as well as some differences in shape among the parentals and hybrids. A detailed inspection using fluorescence microscopy allowed for the identification of shape differences in the cells of the seed coat. In the case of hybrids, differences were found in circularity and solidity. Overal seed shape is maternally regulated for both species, whereas cell shape cannot be associated with any of the sexes.

Discussion

Our results provide additional tools useful for the combination of morphology with genetics, ecology or taxonomy. Seed shape is a robust indicator that can be used as a complementary tool for the genetic and phylogenetic analyses of Silene hybrid populations.
Optimizing rice yield, quality and nutrient use efficiency through combined application of nitrogen and potassium
Authors: Chen Guangyi , Duan Qiang , Wu Chaoyue , He Xingmei , Hu Mingming , Li Congmei , Ouyang Yuyuan , Peng Ligong , Yang Hong , Zhang Qiuqiu , Jiang Qinggui , Lan Yan , Li Tian
Published: March 2024

Reasonable nitrogen (N) and potassium (K) application rates can effectively improve fertilizer use efficiency, rice yield and quality. A two-year field experiment was conducted with combined application of three N rates (135, 180, and 225 kg ha-1, denoted as N1-N3) and four K rates (0, 90, 135, and 180 kg ha-1, denoted as K0-K3) using super indica hybrid rice cultivar Yixiangyou (YXY) 2115 to explore the effects of co-application of N and K on rice growth and development. The results indicated that the combined application of N and K had significantly interactive effects on dry matter (DM) accumulation, nutrients absorption, N harvest index (NHI), K harvest index (KHI), spikelets per panicle and most rice quality indexes. The highest total DM accumulation (17998.17-19432.47 kg ha-1) at maturity stage was obtained under N3K2. The effect of co-application of N and K on nutrients absorption and utilization varied between the two years and within each year. The highest total N and K accumulations at maturity stage were observed under N3K1 and N3K2, respectively, while the highest N recovery efficiency (NRE) and K recovery efficiency (KRE) were observed under N1K3. High expression levels of N and K metabolism-related genes in rice grains were observed under N3K2 or N3K3, consistent with N and K uptake. Co-application of N and K increased rice yield significantly and the highest yield (6745.02-7010.27 kg ha-1) was obtained under N2K2. As more N was gradually applied, rice appearance quality improved but milling, cooking and eating quality decreased. Although appropriate application of K could improve rice milling, cooking and eating quality, it reduced appearance quality. The optimal milling, cooking and eating quality were obtained under N1K2, while the best appearance quality was obtained under N3K0. Overall, a combination of 135-210 kg ha-1 N and 115-137 kg ha-1 K application rates was recommended for achieving relatively higher yield and better quality in rice production.
Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea
Authors: Gali Krishna Kishore , Jha Ambuj , Tar’an Bunyamain , Burstin Judith , Aubert Gregoire , Bing Dengjin , Arganosa Gene , Warkentin Thomas D
Published: March 2024

Improving the seed protein concentration (SPC) of pea (Pisum sativum L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries. To support the marker-assisted selection (MAS) of SPC towards accelerated breeding of improved cultivars, we have explored two diverse recombinant inbred line (RIL) populations to identify the quantitative trait loci (QTLs) associated with SPC. The two RIL populations, MP 1918 × P0540-91 (PR-30) and Ballet × Cameor (PR-31), were derived from crosses between moderate SPC × high SPC accessions. A total of 166 and 159 RILs of PR-30 and PR-31, respectively, were genotyped using an Axiom® 90K SNP array and 13.2K SNP arrays, respectively. The RILs were phenotyped in replicated trials in two and three locations of Saskatchewan, Canada in 2020 and 2021, respectively, for agronomic assessment and SPC. Using composite interval mapping, we identified three QTLs associated with SPC in PR-30 and five QTLs in PR-31, with the LOD value ranging from 3.0 to 11.0. A majority of these QTLs were unique to these populations compared to the previously known QTLs for SPC. The QTL SPC-Ps-5.1 overlapped with the earlier reported SPC associated QTL PC-QTL-3. Three QTLs, SPC-Ps-4.2, SPC-Ps-5.1, and SPC-Ps-7.2 with LOD scores of 7.2, 7.9, and 11.3, and which explained 14.5%, 11.6%, and 11.3% of the phenotypic variance, respectively, can be used for marker-assisted breeding to increase SPC in peas. Eight QTLs associated with the grain yield were identified with LOD scores ranging from 3.1 to 8.2. Two sets of QTLs, SPC-Ps-2.1 and GY-Ps-2.1, and SPC-Ps-5.1 and GY-Ps-5.3, shared the QTL/peak regions. Each set of QTLs contributed to either SPC or grain yield depending on which parent the QTL region is derived from, thus confirming that breeding for SPC should take into consideration the effects on grain yield.