bluuebunny/crossref_metadata_2025 huggingface dataset:
Discrimination of Lipogenic or Glucogenic Diet Effects in Early-Lactation Dairy Cows Using Plasma Metabolite Abundances and Ratios in Combination with Machine Learning
Authors: Wang Xiaodan , Jahagirdar Sanjeevan , Bakker Wouter , Lute Carolien , Kemp Bas , Knegsel Ariette van , Saccenti Edoardo
Published: April 2024

During early lactation, dairy cows have a negative energy balance since their energy demands exceed their energy intake: in this study, we aimed to investigate the association between diet and plasma metabolomics profiles and how these relate to energy unbalance of course in the early-lactation stage. Holstein-Friesian cows were randomly assigned to a glucogenic (n = 15) or lipogenic (n = 15) diet in early lactation. Blood was collected in week 2 and week 4 after calving. Plasma metabolite profiles were detected using liquid chromatography–mass spectrometry (LC-MS), and a total of 39 metabolites were identified. Two plasma metabolomic profiles were available every week for each cow. Metabolite abundance and metabolite ratios were used for the analysis using the XGboost algorithm to discriminate between diet treatment and lactation week. Using metabolite ratios resulted in better discrimination performance compared with the metabolite abundances in assigning cows to a lipogenic diet or a glucogenic diet. The quality of the discrimination of performance of lipogenic diet and glucogenic diet effects improved from 0.606 to 0.753 and from 0.696 to 0.842 in week 2 and week 4 (as measured by area under the curve, AUC), when the metabolite abundance ratios were used instead of abundances. The top discriminating ratios for diet were the ratio of arginine to tyrosine and the ratio of aspartic acid to valine in week 2 and week 4, respectively. For cows fed the lipogenic diet, choline and the ratio of creatinine to tryptophan were top features to discriminate cows in week 2 vs. week 4. For cows fed the glucogenic diet, methionine and the ratio of 4-hydroxyproline to choline were top features to discriminate dietary effects in week 2 or week 4. This study shows the added value of using metabolite abundance ratios to discriminate between lipogenic and glucogenic diet and lactation weeks in early-lactation cows when using metabolomics data. The application of this research will help to accurately regulate the nutrition of lactating dairy cows and promote sustainable agricultural development.
Design of Far-Infrared High-Efficiency Polarization-Independent Retroreflective Metasurfaces
Authors: Zhou Siliang , Dong Siyu , He Tao , Zhu Jingyuan , Wang Zhanshan , Cheng Xinbin
Published: April 2024

Retroreflective gratings serve as fundamental optical elements in nanophotonics, with polarization-independent diffraction efficiency being one of the critical parameters for assessing their performance. In the far-infrared spectral range, traditional retroreflective gratings typically refer to metal echelette gratings, but their diffraction efficiency cannot approach 100% due to metal absorption. In the visible and near-infrared spectral ranges, metal echelette gratings have gradually been replaced by all-dielectric metasurfaces because dielectric materials exhibit negligible absorption at specific wavelengths. However, there is still a lack of relevant research in the far-infrared range, mainly due to the weak control capability of the existing devices over the polarization-independent phase. Here, we propose a kind of all-dielectric retroreflective metasurface composed of asymmetric pillars and freely tunable aperiodic multilayer films. The pillar structure can achieve polarization insensitivity, and the insufficient modulation capability of the dielectric materials can be compensated for by aperiodic Ge/ZnS films. The designed metasurface achieves the diffraction efficiency by RCWA, with the maximum larger than 99% and the overall reaching 95% (9.3–9.6 µm). We have provided detailed explanations of the design methodology and fabrication process. Our work lays the groundwork for further exploration and application of far-infrared lasers.
Design and Simulation of a 19-Electrode MEMS Piezoelectric Thin-Film Micro-Deformable Mirror for Ophthalmology
Authors: Hu Yisen , Yin Hongbo , Li Maoying , Bai Tianyu , He Liang , Hu Zhimin , Xia Yuanlin , Wang Zhuqing
Published: April 2024

This study presents a numerical simulation-based investigation of a MEMS (micro-electromechanical systems)technology-based deformable mirror employing a piezoelectric film for fundus examination in adaptive optics. Compared to the classical equal-area electrode arrangement model, we optimize the electrode array for higher-order aberrations. The optimized model centralizes electrodes around the mirror center, which realizes low-voltage driving with high-accuracy correction. The optimized models exhibited commendable correction abilities, achieving a unidirectional displacement of 5.74 μm with a driven voltage of 15 V. The voltage–displacement relationship demonstrated high linearity at 0.99. Furthermore, the deformable mirror’s influence matrix was computed, aligning with the Zernike standard surface shape of the order 1–3. To quantify aberration correction capabilities, fitting residuals for both models were calculated. The results indicate an average removal of 96.8% of aberrations to the human eye. This underscores that the optimized model outperforms the classical model in correcting high-order aberrations.
Altered Cytostructure and Lignolytic Enzymes of Ganoderma boninense in Response to Phenolic Compounds
Authors: Siddiqui Yasmeen , Ganapathy Daarshini
Published: April 2024

Ganoderma boninense is a white-rot fungus that causes basal stem rot (BSR) disease in the oil palm. Potential natural inhibitors, such as gallic acid, thymol, propolis, and carvacrol, were assessed for their antagonistic effects against G. boninense. These naturally occurring phenolic compounds have also been utilised to inhibit hydrolytic and ligninolytic enzymes produced by the pathogen. Mycelial inhibition was dose-dependent in the presence of different concentrations of phenolic compounds, including, for example, in cellulase enzyme inhibition (GA mg/mL = 94%, THY 0.25 mg/mL = 90%, PRO 3.5 mg/mL = 92.5%, and CARV 0.15 mg/mL = 90.3%). A significant difference was observed revealing that gallic acid had the greatest inhibitory effect on the secretion of hydrolytic and ligninolytic enzymes, especially at 40 mM GA (cellulase = 0.337 U/mL, amylase = 0.3314 U/mL, xylanase = 0.211 U/mL, laccase = 0.4885 U/mL, lignin peroxidase = 0.218 U/mL, and manganese peroxidase = 0.386 U/mL). The growth and secretion of enzymes (inhibitory action) are inversely proportional to the concentration of phenolic compounds. Phenolic compounds have a greater potential as inhibitory agents and suppress the production of hydrolytic and ligninolytic enzymes. The selected phenolic compounds were evaluated for their ability to alter the morphology and integrity of G. boninense mycelia. The reduction in cell viability of G. boninense has been explained by research on morphological disruption, such as branching patterns, hyphal length, and rigidity of fungal cells, which eventually interrupt the secretion of enzymes. These studies highlight the efficacy of phenolic compounds in treating Ganoderma. In addition, these findings proved that naturally occurring phenolic compounds could be a substitute for chemical controls and other synthetic fungicides to eradicate the occurrence of BSR in oil palms, thus avoiding a situation that is difficult to overcome.
On-Line pH Measurement Cation Exchange Kinetics of Y3+-Exchanged Alginic Acid for Y2O3 Nanoparticles Synthesis
Authors: Liu Lingyu , Zhou Fengchen , Zhang Yuxiang , Sun Yanhua , Zhang Shixing , Cai Kun , Qiu Ruichong , Lin Yi , Fa Wenjun , Wang Zihua
Published: April 2024

A new sol-gel method that employs cation exchange from an aqueous metal ion solution with H+ ions of granulated alginic acid was developed for synthesizing high-purity Y2O3 nanoparticles. In this study, the cation exchange kinetics of H+~Y3+ in aqueous solution were analyzed using on-line pH technology and off-line inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis. Pseudo 2nd-order models were utilized to evaluate the parameters of the kinetics, suggesting that the concentration of H+~Y3+ involved in the cation exchange reaction was 1:1.733. Further, a comprehensive understanding of the Y-ALG calcination process was developed using thermo-gravimetric analysis, along with results obtained from differential scanning calorimetry (TGA/DSC). A detailed analysis of the XRD Rietveld refinement plots revealed that the crystallite sizes of Y2O3 nanoparticles were about 4 nm (500 °C) and 15 nm (800 °C), respectively. Differential pulse voltammetry (DPV) was employed to investigate the electrochemical oxidation of catechol. The oxidation peak currents of catechol at Y2O3 (500 °C)/GCE and Y2O3 (800 °C)/GCE showed two stages linear function of concentration (2.0~20.0 × 10−6 mol/L, 20.0~60.0 × 10−6 mol/L). The results indicated that the detection limits were equal to 2.4 × 10−7 mol/L (Y2O3 (500 °C)/GCE) and 7.8 × 10−7 mol/L (Y2O3 (800 °C)/GCE). The study not only provided a method to synthesize metal oxide, but also proposed a promising on-line pH model to study cation exchange kinetics.
Food-Related Behavioral Patterns in Patients with Inflammatory Bowel Diseases: The Role of Food Involvement and Health Engagement
Authors: Palamenghi Lorenzo , Usta Dilara , Leone Salvo , Graffigna Guendalina
Published: April 2024

Nutrition has been acknowledged as crucial in IBD and is relevant to patients’ motives behind food choices, which are affected by health engagement (HE) and food involvement (FI). This study aimed to profile IBD patients according to their levels of health engagement and food involvement to identify patterns of different motives behind food choices, particularly regarding the use of food to regulate mood. A cross-sectional study was conducted with 890 Italian IBD patients who completed an online survey in April 2021. We measured health engagement, food involvement, motives behind food choices, emotional states, and food-related quality of life (Fr-QoL). K-means cluster analysis was performed to identify participants with similar levels of health engagement and food involvement. Four clusters were identified: “Health-conscious (high HE, low FI)”, “Balanced (high HE, high FI)”, “Hedonist (high FI, low HE)”, and “Careless (low FI, low HE)”. Clusters with high FI are inclined toward seeking pleasurable food, but when supported with high health engagement, individuals were less prone to use food to manage mood. Groups with higher health engagement demonstrated lower hospitalization rates and relapses and better Fr-QoL. Profiling IBD patients regarding FI and HE could aid clinicians in identifying individuals at greater risk of maladaptive food-related behaviors.
Effects of Taraxaci Herba (Dandelion) on Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Rats
Authors: Lee Seong Min , Lee Sang Mok , Song Jungbin
Published: April 2024

Benign prostatic hyperplasia (BPH) is the non-malignant enlargement of the prostate, associated with lower urinary tract symptoms (LUTSs). Taraxaci Herba (TH), commonly known as dandelion, has traditionally been utilized in East Asia to treat symptoms related to LUTSs. Based on this traditional use, our study aimed to explore the inhibitory effects of TH on BPH progression using a testosterone propionate-induced rat model. To induce BPH, male Sprague Dawley rats were castrated and injected subcutaneously with testosterone propionate (3 mg/kg/day) for 28 days. Concurrently, TH extract was administered orally at doses of 100 and 300 mg/kg/day throughout the four-week period of testosterone propionate injections. The TH extract significantly reduced both the absolute and relative weights of the prostate, along with histopathological changes in the gland. Moreover, it lowered serum levels of testosterone and dihydrotestosterone and reduced the expression of the androgen receptor in the prostate. Additionally, the TH extract modulated the protein expressions of Bax and Bcl-2, which are key regulators of apoptosis in prostate cells. Collectively, our findings suggest that TH inhibits BPH development partially by modulating androgen signaling and inducing apoptosis within the prostate.
Starvation in Mice Induces Liver Damage Associated with Autophagy
Authors: Schuster Katharina , Staffeld Anna , Zimmermann Annelie , Böge Natalie , Lang Stephan , Kuhla Angela , Frintrop Linda
Published: April 2024

Anorexia nervosa (AN) induces organ dysfunction caused by malnutrition, including liver damage leading to a rise in transaminases due to hepatocyte damage. The underlying pathophysiology of starvation-induced liver damage is poorly understood. We investigate the effect of a 25% body weight reduction on murine livers in a mouse model and examine possible underlying mechanisms of starvation-induced liver damage. Female mice received a restricted amount of food with access to running wheels until a 25% weight reduction was achieved. This weight reduction was maintained for two weeks to mimic chronic starvation. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured spectrophotometrically. Liver fat content was analyzed using an Oil Red O stain, and liver glycogen was determined using a Periodic acid–Schiff (PAS) stain. Immunohistochemical stains were used to investigate macrophages, proliferation, apoptosis, and autophagy. Starvation led to an elevation of AST and ALT values, a decreased amount of liver fat, and reduced glycogen deposits. The density of F4/80+ macrophage numbers as well as proliferating KI67+ cells were decreased by starvation, while apoptosis was not altered. This was paralleled by an increase in autophagy-related protein staining. Increased transaminase values suggest the presence of liver damage in the examined livers of starved mice. The observed starvation-induced liver damage may be attributed to increased autophagy. Whether other mechanisms play an additional role in starvation-induced liver damage remains to be investigated.
IL-33 Reduces Saturated Fatty Acid Accumulation in Mouse Atherosclerotic Foci
Authors: Hosomi Yukako , Okamura Takuro , Sakai Kimiko , Yuge Hiroki , Yoshimura Takashi , Majima Saori , Okada Hiroshi , Senmaru Takafumi , Ushigome Emi , Nakanishi Naoko , Satoh Takashi , Akira Shizuo , Hamaguchi Masahide , Fukui Michiaki
Published: April 2024

The cellular and molecular mechanisms of atherosclerosis are still unclear. Type 2 innate lymphocytes (ILC2) exhibit anti-inflammatory properties and protect against atherosclerosis. This study aimed to elucidate the pathogenesis of atherosclerosis development using atherosclerosis model mice (ApoE KO mice) and mice deficient in IL-33 receptor ST2 (ApoEST2 DKO mice). Sixteen-week-old male ApoE KO and ApoEST2 DKO mice were subjected to an 8-week regimen of a high-fat, high-sucrose diet. Atherosclerotic foci were assessed histologically at the aortic valve ring. Chronic inflammation was assessed using flow cytometry and real-time polymerase chain reaction. In addition, saturated fatty acids (palmitic acid) and IL-33 were administered to human aortic endothelial cells (HAECs) to assess fatty acid metabolism. ApoEST2 DKO mice with attenuated ILC2 had significantly worse atherosclerosis than ApoE KO mice. The levels of saturated fatty acids, including palmitic acid, were significantly elevated in the arteries and serum of ApoEST2 DKO mice. Furthermore, on treating HAECs with saturated fatty acids with or without IL-33, the Oil Red O staining area significantly decreased in the IL-33-treated group compared to that in the non-treated group. IL-33 potentially prevented the accumulation of saturated fatty acids within atherosclerotic foci.
Bupleurum in Treatment of Depression Disorder: A Comprehensive Review
Authors: Ran Shuzhen , Peng Rui , Guo Qingwan , Cui Jinshuai , Chen Gang , Wang Ziying
Published: April 2024

The incidence of depression has been steadily rising in recent years, making it one of the most prevalent mental illnesses. As the pursuit of novel antidepressant drugs captivates the pharmaceutical field, the therapeutic efficacy of Traditional Chinese Medicine (TCM) has been widely explored. Chaihu (Bupleurum) has been traditionally used for liver conditions such as hepatitis, liver inflammation, liver fibrosis, and liver cancer. It is believed to have hepatoprotective effects, promoting liver cell regeneration and protecting against liver damage. In addition, Bupleurum has also been used as a Jie Yu (depression-relieving) medicine in China, Japan, Republic of Korea, and other Asian countries for centuries. This review article aims to summarize the research conducted on the antidepressant properties and mechanisms of Bupleurum, as well as discuss the potential of TCM formulas containing Bupleurum. This review highlights various antidepressant ingredients isolated from Bupleurum, including saikosaponin A, saikosaponin D, rutin, puerarin, and quercetin, each with distinct mechanisms of action. Additionally, Chinese herb prescriptions and extracts containing Bupleurum, such as Chaihu Shugansan, Xiaoyaosan, and Sinisan, are also included due to their demonstrated antidepressant effects. This review reveals that these Bupleurum compounds exhibit antidepressant effects through the regulation of neurotransmitter mechanisms (such as 5-HT and DA), the NMDA (N-methyl-D-aspartate) system, brain-derived neurotrophic factor (BDNF), and other intracellular signaling pathways. Collectively, this comprehensive review provides insights into the multiple applications of Bupleurum in the treatment of depression and highlights its potential as an alternative or complementary approach to traditional therapies. However, it is essential to consider the potential adverse effects and clinical restrictions of Bupleurum despite its promising potential. Further research is needed to elucidate its specific mechanisms of action and evaluate its effectiveness in human subjects.
Human ABC and SLC Transporters: The Culprit Responsible for Unspecific PSMA-617 Uptake?
Authors: Taş Harun , Bakos Gábor , Bauder-Wüst Ulrike , Schäfer Martin , Remde Yvonne , Roscher Mareike , Benešová-Schäfer Martina
Published: April 2024

[177Lu]Lu-PSMA-617 has recently been successfully approved by the FDA, the MHRA, Health Canada and the EMA as Pluvicto®. However, salivary gland (SG) and kidney toxicities account for its main dose-limiting side-effects, while its corresponding uptake and retention mechanisms still remain elusive. Recently, the presence of different ATP-binding cassette (ABC) transporters, such as human breast cancer resistance proteins (BCRP), multidrug resistance proteins (MDR1), multidrug-resistance-related proteins (MRP1, MRP4) and solute cassette (SLC) transporters, such as multidrug and toxin extrusion proteins (MATE1, MATE2-K), organic anion transporters (OAT1, OAT2v1, OAT3, OAT4) and peptide transporters (PEPT2), has been verified at different abundances in human SGs and kidneys. Therefore, our aim was to assess whether [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 are substrates of these ABC and SLC transporters. For in vitro studies, the novel isotopologue ([α,β-3H]Nal)Lu-PSMA-617 was used in cell lines or vesicles expressing the aforementioned human ABC and SLC transporters for inhibition and uptake studies, respectively. The corresponding probe substrates and reference inhibitors were used as controls. Our results indicate that [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 are neither inhibitors nor substrates of the examined transporters. Therefore, our results show that human ABC and SLC transporters play no central role in the uptake and retention of [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 in the SGs and kidneys nor in the observed toxicities.
Proteomic Analysis of Arachis hypogaea Seeds from Different Maturity Classes
Authors: Cherry Ashley , Fisher Brian , Branch William , Peralta Christopher , Gilliam Lissa , Pahom Olga , Liebold Chris , Marshall Julie
Published: April 2024

Physiological maturity impacts seed quality through various mechanisms including vigor, desiccation tolerance, dormancy induction, synthesis of raw materials (including seed storage proteins), and the reorganization of metabolisms. Peanut seed development can be classified into seven classes with four incremental stages per class. Based on the mesocarp color, the final three stages are commonly referred to as “orange”, “brown”, and “black”. In 2017, freshly harvested pods from one genotype of runner market-type peanuts grown under conventional practices were obtained from the University of Georgia research facility. The pods were removed from the plant material and ‘pod blasted’ to reveal the mesocarp. After separation, the remainder of the pod outer layer was removed, and the seeds were segregated for proteomic analysis. The raw peanuts were analyzed by bottom-up LC-MS/MS proteomics, which was conducted by the Proteomics Resource Center at the Rockefeller University, to identify the significant protein composition differences in each maturity class. The proteomic data revealed differentially expressed proteins as a function of maturity class with multiple functions including plant defense, metabolism, cell signaling, nutrient accumulation, and packaging. Understanding the processes needed for seed maturation will enable peanut scientists to evaluate the traits needed for robust germination, hardiness of the seed in response to disease, and nutrient quality.
Relationship between Phenolic Compounds and Antioxidant Activity of Some Moroccan Date Palm Fruit Varieties (Phoenix dactylifera L.): A Two-Year Study
Authors: Ouamnina Abdoussadeq , Alahyane Abderrahim , Elateri Imane , Boutasknit Abderrahim , Abderrazik Mohamed
Published: April 2024

In Morocco, the abundance of low-value varieties in the oases may provide an opportunity to capitalize on this richness to create new nutraceutical food products. In this context, the phenolic profile and antioxidant capacity of four Moroccan date varieties were analyzed. Our results indicate that the levels of total polyphenols, total flavonoids and total condensed tannins vary, respectively, from 91.86 to 364.35 mg GAE/100 g of dry weight (DW), 46.59 to 111.80 mg QE/100 g DW and 16.10 to 42.03 mg CE/100 g DW during the 2021 harvest season. Furthermore, during the 2022 harvest season, these contents vary, respectively, from 119.13 to 410.39 mg GAE/100 g DW, 59.30 to 110.85 mg QE/100 g DW and 21.93 to 53.95 mg CE/100 g DW. The results of the HPLC-UV-VIS analysis revealed that, in all four varieties, gallic acid was and remained one of the major compounds in the date extracts. In addition, a high antioxidant activity of date extracts was particularly observed in the three tests, namely ferric reducing power (FRAP), ferrous ion chelating capacity (FIC) and the phosphomolybdate test. This richness in phenolic compounds makes low-value dates a source of active ingredient that can replace the synthetic antioxidants used in the food and pharmaceutical industries.
Understanding the Saffron Corm Development—Insights into Histological and Metabolic Aspects
Authors: Pallotti Claudia , Renau-Morata Begoña , Cardone Loriana , Nebauer Sergio G. , Albiñana Palacios Mireia , Rivas-Sendra Alba , Seguí-Simarro José M. , Molina Rosa V.
Published: April 2024

The reproduction of Crocus sativus L., a sterile triploid plant, is carried out exclusively through corms, whose size determines the saffron yield. The development of daughter corms (DC) is supported by photoassimilates supplied by the leaves as well as by the mother corms (MC). While biomass partitioning during DC development is well studied, growth dynamics in terms of cell number and size, the involved meristems, as well as carbohydrate partition and allocation, are not yet fully understood. We conducted a comprehensive study into saffron corm growth dynamics at the macroscopic and microscopic levels. Variations in carbohydrate content and enzymatic activities related to sucrose metabolism in sources and sinks were measured. Two key meristems were identified. One is involved in vascular connections between DC and MC. The other is a thickening meristem responsible for DC enlargement. This research explains how the previously described phases of corm growth correlate with variations in cell division, enlargement dynamics, and carbohydrate partitioning among organs. Results also elucidated that the end of DC growth relates to a significant drop in MC root biomass, limiting the water supply for the DC growth, and establishing the onset of leaf wilting. The lack of starch accumulation in aged leaf cells is noteworthy, as is the accumulation of lipids. We hypothesize a signaling role of sugars in DC growth initiation, stop, and leaf aging. Finally, we established a predominant role of sucrose synthase as a sucrolytic enzyme in the maintenance of the high flux of carbon for starch synthesis in DC. Together, the obtained results pave the way for the definition of strategies leading to better control of saffron corm development.
Effect of Amino Silicone Oil-Phosphorylation Hybrid Modification on the Properties of Microcellulose Fibers
Authors: Yuan Quan , Zhang Guimei , Li Chunxuan , Xu Shiwei , He Liping
Published: April 2024

Microcellulose materials are increasingly considered multifunctional candidates for emerging energy applications. Microcellulose fibers (MCF) are a kind of bio-based reinforcement in composites, and their hydrophilic character hinders their wide application in industry. Thus, in the present work, MCF was hybrid-modified by amino silicone oil-phosphorylated to fabricate hydrophobic, thermal stability, and flame-retardant microcellulose fibers for potential application in vehicle engineering. The results showed that the amino silicone oil-phosphorylated (ASOP) hybrid modification could transform the surface property of microcellulose from hydrophilic to hydrophobic and improve the compatibility between MCF and resin matrix. Meanwhile, the ASOP treatment led to the formation of an amino silicone oil film layer on the surface of the microcellulose, which improved the thermal stability of the MCF. Furthermore, the ASOP hybrid modification microcellulose fibers paper (100% microcellulose fibers paper) was transformed from flammable to flame-retardant and showed self-extinguishing behavior after burning under flame for 2 s. The flame-retardant mechanism was attributed to the formation of the char layer in the condensed phase and the production of non-combustible gases in the gaseous phase.
Recycled Polypropylene/Strontium Ferrite Polymer Composite Materials with Electromagnetic Shielding Properties
Authors: Caramitu Alina Ruxandra , Lungu Magdalena Valentina , Ciobanu Romeo Cristian , Ion Ioana , Marin Mihai , Marinescu Virgil , Pintea Jana , Aradoaei Sebastian , Schreiner Oliver Daniel
Published: April 2024

This paper presents the obtaining and characterization of recycled polypropylene/strontium ferrite (PP/SrFe12O19) polymer composite materials with applications in the electromagnetic shielding of vehicle interiors (mainly automotive electronics—carcasses) from the electromagnetic radiation emitted mainly by exterior sources—electrical lines and supply sources—in terms of the development of the new electrical vehicles. With this aim, suitable polymer composite materials were developed using SrFe12O19 filler in two forms (powder and concentrate). The recycled PP polymer and composite materials with a PP/SrFe12O19 weight ratio of 75/25 and 70/30 were obtained in two stages, i.e., pellets by extrusion and samples for testing through a melt injection process. The characterization of the obtained materials took into account the requirements imposed by the desired applications. It consisted of determining the mechanical and dielectric properties, and microstructure analyses, along with the determination of the resistance to the action of a temperature of 70 °C, which is higher than the temperatures created during the summer inside vehicles. The performance of these materials as electromagnetic shields was assessed through functional tests consisting of the determination of magnetic permeability and the estimation of the electromagnetic shielding efficiency (SE). The obtained results confirmed the improvement of the mechanical, dielectric, and magnetic properties of the PP/SrFe12O19 composites compared to the selected PP polymers. It is also found that all the composite materials exhibited reflective shielding properties (SER from −71.5 dB to −56.7 dB), with very little absorption shielding. The most performant material was the composite made of PP/SrFe12O19 powder with a weight ratio of 70/30. The promising results recommend this composite material for potential use in automotive shielding applications against electromagnetic pollution.
Νovel Polylactic Acid/Tetraethyl Citrate Self-Healable Active Packaging Films Applied to Pork Fillets’ Shelf-Life Extension
Authors: Karabagias Vassilios K. , Giannakas Aris E. , Andritsos Nikolaos D. , Moschovas Dimitrios , Karydis-Messinis Andreas , Leontiou Areti , Avgeropoulos Apostolos , Zafeiropoulos Nikolaos E. , Proestos Charalampos , Salmas Constantinos E.
Published: April 2024

Nowadays, increased food safety and decreased food waste are two of the major global interests. Self-healable active packaging materials are an attractive option to achieve such targets. This property is critical for the hygiene and the consumption appropriateness of the food. Polylactic acid is a very promising polymeric matrix that potentially could replace the widely used low-density polyethylene due to its biobased origin and its easy biodegradable nature. The main drawback of this polymeric matrix is its brittle, fragile nature. On the other hand, tetraethyl citrate is a biobased approved food additive which became an attractive option as a plasticizer for industries seeking alternative materials to replace the traditional petrochemically derived compounds. A novel biobased film exhibiting self-healing behavior suitable for food-active packaging was developed during this study. Polylactic acid’s brittleness was reduced drastically by incorporating tetraethyl citrate, and a random cut on the original self-repairing film was fully healed after 120 s. The optimum concentration of tetraethyl citrate in the polylactic acid was around 15% v/w with a water/oxygen barrier close to the relevant of polylactic acid and low migration. According to the EC50 parameter, the antioxidant activity was 300% higher than the relevant of pure polylactic acid, while according to the thiobarbituric acid and heme iron parameters, the film resisted lipid oxidation and deterioration. Finally, the total viable count parameter indicates the strong antimicrobial activity of this sample.
Preparation of a Lignin-Based Cationic Flocculant and Its Application in Kaolin Suspension Treatment
Authors: Li Yan , Yao Suling , Dong Xianshu , Fan Yuping , Ma Xiaomin , Zhu Benkang , Chang Ming
Published: April 2024

The preparation of an environmentally friendly and efficient flocculant for solid-liquid separation in industrial wastewater is highly important. In this study, a novel cationic flocculant (AL-g-PAMA) was synthesized by a thermal initiation method using alkali lignin (AL) as the main chain and acrylamide (AM) and methacrylamido propyl trimethyl ammonium chloride (MAPTAC) as the grafted side chains. The structure, thermal stability, and surface morphology of the copolymers were investigated by various characterization methods. The results indicated the successful synthesis of AL-g-PAMA. AL-g-PAMA was applied to improve solid-liquid separation in kaolin suspensions. The results showed that AL-g-PAMA had excellent flocculation-sedimentation and dewatering efficiency. When the dosage of AL-g-PAMA #5 was 600.0 g/t(s), the thickness of the compressed layer was 2.2 cm, the floc settling velocity was 24.1 cm/min, and the transmittance of the supernatant was 84.0%. The moisture content of the filter cake decreased from 55.0% to 43.4% after treatment with AL-g-PAMA #5. The results of zeta potential and focused beam reflectance measurement (FBRM) analysis indicated that bridging and electroneutralization were the main flocculation mechanisms. Therefore, this study extends the potential for using lignin as a bioflocculant and provides a feasible approach to efficiently purify high-turbidity wastewater.
Biofabrication Approaches for Peri-Implantitis Tissue Regeneration: A Focus on Bioprinting Methods
Authors: Shopova Dobromira , Mihaylova Anna , Yaneva Antoniya , Bakova Desislava , Dimova-Gabrovska Mariana
Published: April 2024

Dental implant utilization has emerged as a contemporary strategy for rectifying dental arch anomalies. However, the effective management of potential complications is paramount. Peri-implantitis, characterized by inflammation and bone resorption around dental implants, resembles periodontitis but specifically affects implant sites. Restoring lost peri-implant tissues poses a multifaceted challenge, with bioprinting methods showing promise as a viable solution. Three-dimensional bioprinting represents a forefront advancement in tissue engineering, traditionally focusing on scaffolds, cells, and signaling pathways. This systematic review aims to aggregate and synthesize data concerning bioprinting’s application in peri-implantitis treatment. Adhering to PRISMA guidelines, the review conducted an extensive literature search across PubMed, Scopus, Google Scholar, and ScienceDirect. Importantly, the search timeframe was not limited, reflecting the scarcity of available information on the subject. Bioprinting advancements offer auspicious avenues for refining treatment modalities, prompting clinicians to explore optimal solutions for establishing ideal anatomical conditions. In essence, this systematic review underscores 3D bioprinting’s potential in peri-implantitis management, highlighting its pivotal role in contemporary dental medicine and its capacity to reshape clinical approaches toward achieving optimal outcomes.
Depressive Disorders and EU Labour Resources—The Problem of Lost Productivity in Highly Developed Economies
Authors: Jakubowska Agnieszka
Published: April 2024

Research has shown that mental disorders in working-age individuals are associated with a significant burden of disability, sickness absence, and presenteeism, resulting in lost productivity. This study aims to assess the impact of depressive disorders on the labour markets in the European Union countries, with a particular focus on the disparities between the ‘old’ and ‘new’ EU countries. The study analysed the process of convergence of the burden of depressive disorders among people aged 20–54 in the group of EU-27 countries in the years 1990–2019, using data from the Global Burden of Disease Study 2019. The initial hypothesis of persistent inequalities in the system studied was verified. The study measured years lived with disability (YLD) due to depressive disorders in the working-age population. The analysis revealed that depressive disorders have a significant impact on the highly developed economies of the EU-14 countries. The convergence assessment did not confirm the effect of catching up with more favourable economies over time, and the inequalities observed at the beginning of the analysis period worsened. This study’s results expand upon the existing literature on the consequences of depressive disorders by introducing the perspective of inequalities in the studied area. These inequalities were observed between EU-27 economies with varying levels of socio-economic development.
Estimation of Top-of-Atmosphere Longwave Cloud Radiative Forcing Using FengYun-4A Geostationary Satellite Data
Authors: Xu Ri , Zhao Jun , Bao Shanhu , Shang Huazhe , Bao Fangling , Tana Gegen , Wei Lesi
Published: April 2024

The distribution and variation of top-of-atmosphere longwave cloud radiative forcing (LCRFTOA) has drawn a significant amount of attention due to its importance in understanding the energy budget. Advancements in sensor and data processing technology, as well as a new generation of geostationary satellites, such as the FengYun-4A (FY-4A), allow for high spatiotemporal resolutions that are crucial for real-time radiation monitoring. Nevertheless, there is a distinct lack of official top-of-atmosphere outgoing longwave radiation products under clear-sky conditions (OLRclear). Consequently, this study addresses the challenge of constructing LCRFTOA data with high spatiotemporal resolution over the full disk region of FY-4A. After simulating the influence of atmospheric parameters on OLRclear based on the SBDART radiation transfer model (RTM), we developed a model for estimating OLRclear using infrared channels from the advanced geosynchronous radiation imager (AGRI) onboard the FY-4A satellite. The OLRclear results showed an RMSE of 5.05 W/m2 and MBE of 1.59 W/m2 compared to ERA5. The corresponding RMSE and MBE value compared to CERES was 6.52 W/m2 and 2.39 W/m2. Additionally, the calculated LCRFTOA results were validated against instantaneous, daily average, and monthly average ERA5 and CERES LCRFTOA products, supporting the validity of the algorithm proposed in this paper. Finally, the changes in LCRFTOA due to varied cloud heights (high, medium, and low cloud) were analyzed. This study provides the basis for comprehensive studies on the characteristics of top-of atmosphere radiation. The results suggest that high-height clouds exert a greater degree of radiative forcing more frequently, while low-height clouds are more frequently found in the lower forcing range.
Remote, Rugged Field Scenarios for Archaeology and the Field Sciences: Object Avoidance and 3D Flight Planning with sUAS Photogrammetry
Authors: Klehm Carla , Williamson Malcolm D. , Bement Leland C. , Bethke Brandi
Published: April 2024

Advances built into recent sUASs (drones) offer a compelling possibility for field-based data collection in logistically challenging and GPS-denied environments. sUASs-based photogrammetry generates 3D models of features and landscapes, used extensively in archaeology as well as other field sciences. Until recently, navigation has been limited by the expertise of the pilot, as objects, like trees, and vertical or complex environments, such as cliffs, create significant risks to successful documentation. This article assesses sUASs’ capability for autonomous obstacle avoidance and 3D flight planning using data collection scenarios carried out in Black Mesa, Oklahoma. Imagery processed using commercial software confirmed that the collected data can build photogrammetric models suitable for general archaeological documentation. The results demonstrate that new capabilities in drones may open up new field environments previously considered inaccessible, too risky, or costly for fieldwork, especially for all but the most expert pilots. Emerging technologies for drone-based photogrammetry, such as the Skydio 2+ considered here, place remote, rugged terrain within reach of many archaeological research units in terms of commercial options and cost.
Time Series Analysis of Multisensor Data for Precision Viticulture—Assessing Microscale Variations in Plant Development with Respect to Irrigation and Topography
Authors: Brandmeier Melanie , Heßdörfer Daniel , Siebenlist Philipp , Meyer-Spelbrink Adrian , Kraus Anja
Published: April 2024

In the context of climate change, vineyard monitoring to better understand spatiotemporal patterns of grapevine development is of utter importance for precision viticulture. We present a time series analysis of hyperspectral in situ and multispectral UAV data for different irrigation systems in Lower Franconia and correlate results with sensor data for soil moisture, temperature, and precipitation. Analysis of Variance (ANOVA) and a Tukey’s HSD test were performed to see whether Vegetation Indices (VIs) are significantly different with respect to irrigation systems as well as topographic position in the vineyard. Correlation between in situ measurements and UAV data for selected VIs is also investigated for upscaling analysis. We find significant differences with respect to irrigation, as well as for topographic position for most of the VIs investigated, highlighting the importance of adapted water management. Correlation between in situ and UAV data is significant only for some indices (NDVI and CIRedEdge, r2 of 0.33 and 0.49, respectively), while shallow soil moisture patterns correlate well with in situ-derived VIs such as the CIRedEdge and RG index (r2 of 0.34 and 0.46).
Lightweight Deep Neural Network with Data Redundancy Removal and Regression for DOA Estimation in Sensor Array
Authors: Liu Aifei , Guo Jiapeng , Arnatovich Yauhen , Liu Zhiling
Published: April 2024

In this paper, a lightweight deep neural network (DNN) for direction of arrival (DOA) estimation is proposed, of which the input vector is designed to remove data redundancy as well as remaining DOA information. By exploring the Vandermonde property of the steering vector of a uniform linear array (ULA), the size of the newly designed input vector is greatly reduced. Furthermore, the DOA estimation is designed as a regression problem instead of a classification problem; that is, the lightweight DNN designs the output vector as the estimated DOAs of sources, of which the size is much shorter than that of the spatial spectrum used as the output vector in the conventional DNN. The reductions in the sizes of input and output vectors lead to a reduction in the sizes of hidden layers, achieving lightweightness of the neural network. The analysis illustrates that when the number of sensors is 22, the number of parameters in the lightweight DNN is three orders of magnitude less than that in the conventional DNN. The simulation results demonstrate the lightweight DNN can provide high DOA estimation accuracy with the shortest testing time. It performs better than the conventional DNN. Furthermore, it is superior to traditional solutions such as the multiple signal classification (MUSIC) method and conventional beamforming (CBF) method in harsh conditions like low signal-to-noise ratios (SNRs), closely spaced sources, and few snapshots.
Monsoon-Based Linear Regression Analysis for Filling Data Gaps in Gravity Recovery and Climate Experiment Satellite Observations
Authors: Mohasseb Hussein A. , Shen Wenbin , Jiao Jiashuang
Published: April 2024

Over the past two decades, the Gravity Recovery and Climate Experiment (GRACE) satellite mission and its successor, GRACE-follow on (GRACE-FO), have played a vital role in climate research. However, the absence of certain observations during and between these missions has presented a persistent challenge. Despite numerous studies attempting to address this issue with mathematical and statistical methods, no definitive optimal approach has been established. This study introduces a practical solution using Linear Regression Analysis (LRA) to overcome data gaps in both GRACE data types—mascon and spherical harmonic coefficients (SHCs). The proposed methodology is tailored to monsoon patterns and demonstrates efficacy in filling data gaps. To validate the approach, a global analysis was conducted across eight basins, monitoring changes in total water storage (TWS) using the technique. The results were compared with various geodetic products, including data from the Swarm mission, Institute of Geodesy and Geoinformation (IGG), Quantum Frontiers (QF), and Singular Spectrum Analysis (SSA) coefficients. Artificial data gaps were introduced within GRACE observations for further validation. This research highlights the effectiveness of the monsoon method in comparison to other gap-filling approaches, showing a strong similarity between gap-filling results and GRACE’s SHCs, with an absolute relative error approaching zero. In the mascon approach, the coefficient of determination (R2) exceeded 91% for all months. This study offers a readily usable gap-filling product—SHCs and smoothed gridded observations—with accurate error estimates. These resources are now accessible for a wide range of applications, providing a valuable tool for the scientific community.
Through-Wall Imaging Using Low-Cost Frequency-Modulated Continuous Wave Radar Sensors
Authors: Paun Mirel
Published: April 2024

Many fields of human activity benefit from the ability to create images of obscured objects placed behind walls and to map their displacement in a noninvasive way. Usually, imaging devices like Synthetic Aperture Radars (SARs) and Ground-Penetrating Radars (GPRs) use expensive dedicated electronics which results in prohibitive prices. This paper presents the experimental implementation and the results obtained from an imaging system capable of performing SAR imaging and interferometric displacement mapping of targets located behind walls, as well as 3D GPR imaging using a low-cost general-purpose radar sensor. The proposed solution uses for the RF section of the system a K-band microwave radar sensor module implementing Frequency-Modulated Continuous Wave (FMCW) operation. The low-cost sensor was originally intended for simple presence detection and ranging for domestic applications. The proposed system was tested in several scenarios and proved to operate as intended for a fraction of the cost of a commercial imaging device. In one scenario, it was able to detect and locate a 15 cm-diameter fire-extinguisher located at a distance of 3.5 m from the scanning system and 1.6 m behind a 3 cm-thick MDF (medium-density fiberboard) wall with cm-level accuracy. In a second test, the proposed system was used to perform interferometric displacement measurements, and it was capable of determining the displacement of a metal case with sub-millimeter accuracy. In a third experiment, the system was used to construct a 3D image of the inside of a wood table with cm-level resolution.
Drinking Behaviour of Beef Cattle Subject to Water Medication in Various Environmental Conditions
Authors: Romanzini Eliéder Prates , McCollum Vivienne , Mcilveen Sarah , da Silva Kawane Dias , de Souza William Luiz , Bernardes Priscila Arrigucci , Costa Diogo Fleury Azevedo
Published: April 2024

Two experiments were conducted to assess the effects of water medication technology on beef cattle behaviour and performance in tropical conditions. Experiment 1 involved 30 Droughtmaster yearling steers monitored over seven days in a controlled environment. Feed and water consumptions were monitored with Smart Feed Pro® systems, with three water treatments administered via uDOSE® technology. The results indicated an average water intake of 13.6 L/head/d. Experiment 2 had 120 yearling steers from four genetic groups grazing on an extensive pasture system. Throughout four 24-day periods, forage availability and chemical composition were measured once monthly. Experiment 2 revealed a variation in water intake, ranging from 16.2 L/head/d down to 4.75 L/head/d. Notably, the lower intake coincided with a rainfall event documented during the fourth experimental period. Overall, results from both experiments indicated that water medication did not alter cattle water preference. There was no preference for treated water sources in Experiment 1, while differences in Experiment 2 appeared to be influenced by external factors like weather and prior habits. These experiments demonstrate the feasibility of water medication for beef cattle without disruption of their natural behaviour.