bluuebunny/crossref_metadata_2025 huggingface dataset:
VECTOR: An Integrated Correlation Network Database for the Identification of CeRNA Axes in Uveal Melanoma
Authors: Barbagallo Cristina , Di Maria Antonio , Alecci Adriana , Barbagallo Davide , Alaimo Salvatore , Colarossi Lorenzo , Ferro Alfredo , Di Pietro Cinzia , Purrello Michele , Pulvirenti Alfredo , Ragusa Marco
Published: June 2021

Uveal melanoma (UM) is the most common primary intraocular malignant tumor in adults and, although its genetic background has been extensively studied, little is known about the contribution of non-coding RNAs (ncRNAs) to its pathogenesis. Indeed, its competitive endogenous RNA (ceRNA) regulatory network comprising microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and mRNAs has been insufficiently explored. Thanks to UM findings from The Cancer Genome Atlas (TCGA), it is now possible to statistically elaborate these data to identify the expression relationships among RNAs and correlative interaction data. In the present work, we propose the VECTOR (uVeal mElanoma Correlation NeTwORk) database, an interactive tool that identifies and visualizes the relationships among RNA molecules, based on the ceRNA model. The VECTOR database contains: (i) the TCGA-derived expression correlation values of miRNA-mRNA, miRNA-lncRNA and lncRNA-mRNA pairs combined with predicted or validated RNA-RNA interactions; (ii) data of sense-antisense sequence overlapping; (iii) correlation values of Transcription Factor (TF)-miRNA, TF-lncRNA, and TF-mRNA pairs associated with ChiPseq data; (iv) expression data of miRNAs, lncRNAs and mRNAs both in UM and physiological tissues. The VECTOR web interface can be queried, by inputting the gene name, to retrieve all the information about RNA signaling and visualize this as a graph. Finally, VECTOR provides a very detailed picture of ceRNA networks in UM and could be a very useful tool for researchers studying RNA signaling in UM. The web version of Vector is freely available at the URL reported at the end of the Introduction.
Genome Reorganization during Erythroid Differentiation
Authors: Ryzhkova Anastasia , Battulin Nariman
Published: June 2021

Hematopoiesis is a convenient model to study how chromatin dynamics plays a decisive role in regulation of cell fate. During erythropoiesis a population of stem and progenitor cells becomes increasingly lineage restricted, giving rise to terminally differentiated progeny. The concerted action of transcription factors and epigenetic modifiers leads to a silencing of the multipotent transcriptome and activation of the transcriptional program that controls terminal differentiation. This article reviews some aspects of the biology of red blood cells production with the focus on the extensive chromatin reorganization during differentiation.
Working and Learning during the COVID-19 Confinement: An Exploratory Analysis with a Small Sample from Portugal
Authors: Martins Alexandra Dias , Sobral Sónia Rolland
Published: June 2021

The epidemiological situation caused by the COVID-19 pandemic led to efforts to mitigate the transmission of the infection, forcing workers and students to stay at home. Universities closed, as did many businesses, forcing education and work to adapt to the new situation. While for some people it was a positive experience, for others it was not. This article analyzes the responses of 89 respondents, over 18 years old, in teleworking or enrolled in university online education, in a survey at the beginning of 2021, when Portugal was in a new state of emergency. Variables such as gender, age and parenthood were used, as well as ownership of equipment, own workspace, and quality of internet, comparing distance learning/work with traditional methods and measuring levels of satisfaction and willingness to adopt this model in the future. These results suggest an association of gender and parenting in the valuation of telework/distance education; women were more positive than men and participants with children were more positive than participants without children. The same was the case for respondents with their own workspace and better-quality internet. There is a strong relation between paternity and the preference for the distance model, in the sense of valuing the distance model, as well as a relation between those who have their own work space and the appreciation of the distance model. In general, respondents to our survey showed that they are not fond of adopting telework/distance learning in the future.
Characterization of Anopheles stephensi Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome
Authors: Speth Zachary , Kaur Gurlaz , Mazolewski Devin , Sisomphou Rayden , Siao Danielle Denise C. , Pooraiiouby Rana , Vasquez-Gross Hans , Petereit Juli , Gulia-Nuss Monika , Mathew Dennis , Nuss Andrew B.
Published: June 2021

Several mosquito species within the genus Anopheles are vectors for human malaria, and the spread of this disease is driven by the propensity of certain species to feed preferentially on humans. The study of olfaction in mosquitoes is important to understand dynamics of host-seeking and host-selection; however, the majority of these studies focus on Anopheles gambiae or An. coluzzii, both vectors of malaria in Sub-Saharan Africa. Other malaria vectors may recognize different chemical cues from potential hosts; therefore, in this study, we investigated An. stephensi, the south Asian malaria mosquito. We specifically focused on the mouthparts (primarily the maxillary palp and labella) that have been much less investigated compared to the antennae but are also important for host-seeking. To provide a broad view of chemoreceptor expression, RNAseq was used to examine the transcriptomes from the mouthparts of host-seeking females, blood-fed females, and males. Notably, AsOr8 had a high transcript abundance in all transcriptomes and was, therefore, cloned and expressed in the Drosophila empty neuron system. This permitted characterization with a panel of odorants that were selected, in part, for their presence in the human odor profile. The responsiveness of AsOr8 to odorants was highly similar to An. gambiae Or8 (AgOr8), except for sulcatone, which was detected by AsOr8 but not AgOr8. Subtle differences in the receptor sensitivity to specific odorants may provide clues to species- or strain-specific approaches to host-seeking and host selection. Further exploration of the profile of An. stephensi chemosensory proteins may yield a better understanding of how different malaria vectors navigate host-finding and host-choice.
The Effect of Gut Bacteria on the Physiology of Red Palm Weevil, Rhynchophorus ferrugineus Olivier and Their Potential for the Control of This Pest
Authors: Liu Qian-Xia , Su Zhi-Ping , Liu Hui-Hui , Lu Sheng-Ping , Ma Bing , Zhao Yue , Hou You-Ming , Shi Zhang-Hong
Published: June 2021

Red Palm Weevil (RPW), Rhynchophorus ferrugineus Olivier, is a notorious pest, which infests palm trees and has caused great economic losses worldwide. At present, insecticide applications are still the main way to control this pest. However, pesticide resistance has been detected in the field populations of RPW. Thus, future management strategies based on the novel association biological control need be developed. Recent studies have shown that the intestinal tract of RPW is often colonized by multiple microbial species as mammals and model insects, and gut bacteria have been found to promote the growth, development and immune activity of RPW larvae by modulating nutrient metabolism. Furthermore, two peptidoglycan recognition proteins (PGRPs), PGRP-LB and PGRP-S1, can act as the negative regulators to modulate the intestinal immunity to maintain the homeostasis of gut bacteria in RPW larvae. Here, we summarized the current knowledge on the gut bacterial composition of RPW and their impact on the physiological traits of RPW larvae. In contrast with metazoans, it is much easier to make genetic engineered microbes to produce some active molecules against pests. From this perspective, because of the profound effects of gut bacteria on host phenotypes, it is promising to dissect the molecular mechanisms behind their effect on host physiology and facilitate the development of microbial resource-based management methods for pest control.
Ligusticum sinense Nanoemulsion Gel as Potential Repellent against Aedes aegypti, Anopheles minimus, and Culex quinquefasciatus (Diptera: Culicidae)
Authors: Junkum Anuluck , Maleewong Wanchai , Saeung Atiporn , Champakaew Danita , Chansang Arpaporn , Amornlerdpison Doungporn , Aldred Arunee Kongdee , Chaithong Udom , Jitpakdi Atchariya , Riyong Doungrat , Pitasawat Benjawan
Published: June 2021

Ligusticum sinense Oliv. cv. is a species of Umbelliferae (Apiaceae), a large plant family in the order Apiales. In this study, L. sinense hexane extract nanoemulsion gel (LHE-NEG) was investigated for mosquito repellency and compared to the standard chemical, N,N-diethyl-3-methylbenzamide (DEET), with the goal of developing a natural alternative to synthetic repellents in protecting against mosquito vectors. The results demonstrated that LHE-NEG afforded remarkable repellency against Aedes aegypti, Anopheles minimus, and Culex quinquefasciatus, with median protection times (MPTs) of 5.5 (4.5–6.0), 11.5 (8.5–12.5), and 11.25 (8.5–12.5) h, respectively, which was comparable to those of DEET-nanoemulsion gel (DEET-NEG: 8.5 (7.0–9.0), 12.0 (10.0–12.5), and 12.5 (10.0–13.5) h, respectively). Evaluation of skin irritation in 30 human volunteers revealed no potential irritant from LHE-NEG. The physical and biological stability of LHE-NEG were determined after being kept under heating/cooling cycle conditions. The stored samples of LHE-NEG exhibited some changes in appearance and differing degrees of repellency between those kept for 3 and 6 heating/cooling cycles, thus providing slightly shorter MPTs of 4.25 (4.0–4.5) and 3.25 (2.5–3.5) h, respectively, when compared to those of 5.0 (4.5–6.0) h in fresh preparation. These findings encourage commercially developed LHE-based products as an alternative to conventional synthetic repellents in preventing mosquito bites and helping to interrupt mosquito-borne disease transmission.
Evaluation of Reference Genes and Expression Level of Genes Potentially Involved in the Mode of Action of Cry1Ac and Cry1F in a Susceptible Reference Strain of Chrysodeixis includens
Authors: Martin Macarena , Boaventura Debora , Nauen Ralf
Published: June 2021

Soybean looper (SBL), Chrysodeixis includens (Walker), is one of the major lepidopteran pests of soybean in the American continent. SBL control relies mostly on the use of insecticides and genetically modified crops expressing Bacillus thuringiensis (Bt) insecticidal Cry proteins. Due to the high selection pressure exerted by these control measures, resistance has developed to different insecticides and Bt proteins. Nevertheless, studies on the mechanistic background are still scarce. Here, the susceptibility of the laboratory SBL-Benzon strain to the Bt proteins Cry1Ac and Cry1F was determined in diet overlay assays and revealed a greater activity of Cry1Ac than Cry1F, thus confirming results obtained for other sensitive SBL strains. A reference gene study across larval stages with four candidate genes revealed that RPL10 and EF1 were the most stable genes for normalization of gene expression data obtained by RT-qPCR. Finally, the basal expression levels of eight potential Bt protein receptor genes in six larval instars were analyzed, including ATP-binding cassette (ABC) transporters, alkaline phosphatase, aminopeptidases, and cadherin. The results presented here provide fundamental knowledge to support future SBL resistance studies.
Impact of Wall Materials on Physico-Chemical Properties and Stability of Eggplant Peels Anthocyanin Hydrogels
Authors: Condurache (Lazăr) Nina-Nicoleta , Turturică Mihaela , Enachi Elena , Barbu Vasilica , Bahrim Gabriela-Elena , Stănciuc Nicoleta , Croitoru Constantin , Râpeanu Gabriela
Published: June 2021

In this study, eggplant peel extract was used to obtain hydrogels. Two experimental variants were realized by varying the wall materials. Whey proteins isolate (WPI), citrus pectin (P), and sodium carboxymethylcellulose (CMCNa) were used as wall materials. The microcapsules were obtained by the gelation technique, followed by freeze-drying in order to obtain powders. Both experimental variants were analyzed in terms of phytochemical content, antioxidant activity, storage stability, and in vitro digestibility. Additionally, confocal microscopy was used to observe the encapsulation of the bioactive compounds from the eggplant peel extract into the selected matrices. The encapsulation efficiency of the powders varied from 64.67 ± 0.68% for variant 1 (V1) to 96.44 ± 3.43% for variant 2 (V2). Both powders presented high bioactive compound content with high antioxidant activity. V2 showed the highest stability within 28 days of storage, but also in the simulated digestive system.
Acute Dental Periapical Abscess and New-Onset Atrial Fibrillation: A Nationwide, Population-Based Cohort Study
Authors: Hassan Amaar Obaid , Lip Gregory Y. H. , Bisson Arnaud , Herbert Julien , Bodin Alexandre , Fauchier Laurent , Harris Rebecca V.
Published: June 2021

There are limited data on the relationship of acute dental infections with hospitalisation and new-onset atrial fibrillation (AF). This study aimed to assess the relationship between acute periapical abscess and incident AF. This was a retrospective cohort study from a French national database of patients hospitalized in 2013 (3.4 million patients) with at least five years of follow up. In total, 3,056,291 adults (55.1% female) required hospital admission in French hospitals in 2013 while not having a history of AF. Of 4693 patients classified as having dental periapical abscess, 435 (9.27%) developed AF, compared to 326,241 (10.69%) without dental periapical abscess that developed AF over a mean follow-up of 4.8 ± 1.7 years. Multivariable analysis indicated that dental periapical abscess acted as an independent predictor for new onset AF (p < 0.01). The CHA2DS2VASc score in patients with acute dental periapical abscess had moderate predictive value for development of AF, with Area Under the Curve (AUC) 0.73 (95% CI, 0.71–0.76). An increased risk of new onset AF was identified for individuals hospitalized with dental periapical abscess. Careful follow up of patients with severe, acute dental periapical infections is needed for incident AF, as well as investigations of possible mechanisms linking these conditions.
A Prospective Multicenter Study of “Inside Stents” for Biliary Stricture: Multicenter Evolving Inside Stent Registry (MEISteR)
Authors: Kogure Hirofumi , Kato Hironari , Kawakubo Kazumichi , Ishiwatari Hirotoshi , Katanuma Akio , Okabe Yoshinobu , Ueki Toru , Ban Tesshin , Hanada Keiji , Sugimori Kazuya , Nakai Yousuke , Isayama Hiroyuki
Published: June 2021

Background: Endoscopic biliary stent placement is the standard of care for biliary strictures, but stents across the papilla are prone to duodenobiliary reflux, which can cause stent occlusion. Preliminary studies of “inside stents” placed above the papilla showed encouraging outcomes, but prospective data with a large cohort were not reported. Methods: This was a prospective multicenter registry of commercially available inside stents for benign and malignant biliary strictures. Primary endpoint was recurrent biliary obstruction (RBO). Secondary endpoints were technical success of stent placement and removal, adverse events, and stricture resolution. Results: A total of 209 inside stents were placed in 132 (51 benign and 81 malignant) cases with biliary strictures in 10 Japanese centers. During the follow-up period of 8.4 months, RBO was observed in 19% of benign strictures. The RBO rate was 49% in malignant strictures, with the median time to RBO of 4.7 months. Technical success rates of stent placement and removal were both 100%. The adverse event rate was 8%. Conclusion: This prospective multicenter study demonstrated that inside stents above the papilla were feasible in malignant and benign biliary strictures, but a randomized controlled trial is warranted to confirm its superiority to conventional stents across the papilla.
Subchondral Bone Relative Area and Density in Human Osteoarthritic Femoral Heads Assessed with Micro-CT before and after Mechanical Embedding of the Innovative Multi-Spiked Connecting Scaffold for Resurfacing THA Endoprostheses: A Pilot Study
Authors: Dąbrowski Mikołaj , Rogala Piotr , Uklejewski Ryszard , Patalas Adam , Winiecki Mariusz , Gapiński Bartosz
Published: June 2021

The multi-spiked connecting scaffold (MSC-Scaffold) prototype is the essential innovation in the fixation of components of resurfacing total hip arthroplasty (THRA) endoprostheses in the subchondral trabecular bone. We conducted the computed micro-tomography (micro-CT) assessment of the subchondral trabecular bone microarchitecture before and after the MSC-Scaffold embedding in femoral heads removed during long-stem endoprosthesis total hip arthroplasty (THA) of different bone densities from 4 patients with hip osteoarthritis (OA). The embedding of the MSC-Scaffold in subchondral trabecular bone causes the change in its relative area (BA/TA, bone area/total area ratio) ranged from 18.2% to 24.7% (translating to the calculated density ρB relative change 11.1–14.4%, and the compressive strength S relative change 75.3–122.7%) regardless of its initial density (before the MSC-Scaffold embedding). The densification of the trabecular microarchitecture of subchondral trabecular bone due to the MSC-Scaffold initial embedding gradually decreases with the increasing distance from the apexes of the MSC-Scaffold’s spikes while the spatial extent of this subchondral trabecular bone densification ranged from 1.5 to 2.5 mm (which is about half the height of the MSC-Scaffold’s spikes). It may be suggested, despite the limited number of examined femoral heads, that: (1) the magnitude of the effect of the MSC-Scaffold embedding on subchondral trabecular bone densification may be a factor contributing to the maintenance of the MSC-Scaffold also for decreased initial bone density values, (2) the deeper this effect of the subchondral trabecular bone densification, the better strength of subchondral trabecular bone, and as consequence, the better post-operative embedding of the MSC-Scaffold in the bone should be expected.
Randomized-Controlled Trial Examining the Effect of Pelvic Floor Muscle Training in the Treatment of Stress Urinary Incontinence in Men after a Laparoscopic Radical Prostatectomy Pilot Study
Authors: Strojek Katarzyna , Weber-Rajek Magdalena , Strączyńska Agnieszka , Piekorz Zuzanna , Pilarska Beata , Jarzemski Piotr , Kozakiewicz Mariusz , Brzoszczyk Bartosz , Jarzemski Marcin , Styczyńska Hanna , Goch Aleksander , Radzimińska Agnieszka
Published: June 2021

Aim: The aim of this study was to assess the impact of pelvic floor muscle training (PMFT) in the treatment of stress urinary incontinence (SUI) in men after they received radical prostatectomy (RP). Methods: From November 2018 to September 2019, patients who underwent radical prostatectomy were assessed for eligibility. A total of 37 men were then randomly assigned to the experimental group (EG) and the control group (CG). The EG group received supervised exercise twice a week for 12 weeks, and the CG did not receive any intervention. To objectify the results obtained in both groups before and after the intervention, the authors assessed myostatin concentration. Moreover, the Expanded Prostate Cancer Index Composite (EPIC-26) was applied to assess the quality of life, and Beck’s Depression Inventory (BDI-II) was used to measure depression severity. Results: Study results demonstrated a statistically significant reduction of myostatin concentration in the EG following the treatment and no statistically significant differences in this parameter in the CG. In addition, a comparison of the EPIC-26 scores in the EG at the initial and final assessments revealed a statistically significant improvement in the quality of life in each domain. A comparison of the EPIC-26 scores in the CG at the initial and final assessments showed there is a statistically significant decline in quality of life in the “overall urinary problem” and “sexual” domain. A comparison of the BDI-II scores at the initial and final assessments showed a statistically significant decline in depressive symptoms in the EG and no statistically significant differences in the CG. Conclusions: PFMT is an effective treatment for urinary incontinence (UI) in men who received radical prostatectomy.
Ocular and Systemic Manifestations in Paediatric Multisystem Inflammatory Syndrome Associated with COVID-19
Authors: Lo Tzu-Chen , Chen Yu-Yen
Published: June 2021

This study aimed to achieve a better understanding of the epidemiological and clinical characteristics of multisystem inflammatory syndrome in children (MIS-C) following coronavirus disease 2019 (COVID-19). We searched PubMed and Embase between December 2019 and March 2021 and included only peer-reviewed clinical studies or case series. The proportions of patients who had conjunctivitis, systemic symptoms/signs (s/s), Kawasaki disease (KD), and exposure history to suspected/confirmed COVID-19 cases were obtained. Moreover, positive rates of the nasopharyngeal real-time reverse transcriptase polymerase chain reaction (RT-PCR) and serum antibody for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were recorded. Overall, 32 studies with 1458 patients were included in the pooled analysis. Around half of the patients had conjunctivitis. The five most common systemic manifestations were fever (96.4%), gastrointestinal s/s (76.7%), shock (61.5%), rash (57.1%), and neurological s/s (36.8%). Almost one-third presented complete KD and about half had exposure history to COVID-19 cases. The positivity of the serology (82.2%) was higher than that of the nasopharyngeal RT-PCR (37.0%). MIS-C associated with COVID-19 leads to several features similar to KD. Epidemiological and laboratory findings suggest that post-infective immune dysregulation may play a predominant role. Further studies are crucial to elucidate the underlying pathogenesis.
Physicochemical Property Indexes of Sediment Lixiviums in Sea–Land Interaction Zone of Subei Basin and Their Significance to Transgression
Authors: Shu Qiang , Zhang Shunjie , Chen Ye
Published: June 2021

In current studies, the physicochemical properties of water, such as total dissolved solids, salinity, and electrical conductivity, are used mainly to investigate changes in the properties of surface water and groundwater. In our experimental study, we aimed to introduce the physicochemical properties of water bodies into the field of paleoenvironmental changes. We employed the physicochemical property indexes of sediment lixiviums in two research sections of the sea–land interaction zone in the eastern margin of the Subei Basin (China). Preliminary tests determined that the optimal solvent for preparing the sediment lixiviums is ultrapure water; the use of this water can prevent errors caused by soluble solids in the solvent. Using a container with a lid to prepare the sediment lixiviums could reduce errors caused by evaporation. Furthermore, we determined the appropriate process and duration for testing the physicochemical properties of sediment lixiviums. The optimal time for testing the physicochemical properties was 120 h (mixture fully stirred daily) or 168 h (no stirring). The weight of the sediment, volume of the solvent, and test time should be consistent in the same research section. Comparing the physicochemical property indexes of sediment lixiviums with geochemical elements and diatom indicators, we found that these indexes show obvious indications of transgression, and have an obvious advantage in indicating transgression.
A Very Large Spawning Aggregation of a Deep-Sea Eel: Magnitude and Status
Authors: Williams Alan , Osterhage Deborah , Althaus Franziska , Ryan Timothy , Green Mark , Pogonoski John
Published: June 2021

Multiple lines of evidence substantiate the existence of a very large aggregation of the basketwork eel, Diastobranchus capensis, on the small (3 km2) Patience Seamount off southeast Australia. The aggregation appears to be present year-round, but largest in the austral autumn when composed of spawning eels. Twenty eels caught in April 2015 (14 female, 6 male) were all in advanced stages of spawning condition. The eel’s abundance in the aggregation was very high as measured at seamount, local and regional scales. Hydroacoustic measurement of the spawning aggregation’s dimensions (~100 × 1000 m) and conservative counts of 100 s of eels along camera transects of ~1000–2000 m length indicate 10,000 s individual eels may have been present. The absence of other known spawning locations indicates the Patience Seamount is a regional-scale spatial anchor for spawning. The aggregation was protected in a marine park in 2007 following a decades-long impact from bottom trawling, indicating that the population can be expected to stabilise and recover. Monitoring the aggregation’s status, and validating seasonal spawning, provide important opportunities to examine conservation-led recovery in the deep sea as part of Australia’s new national strategy of Monitoring, Evaluation, Reporting and Improvement (MERI) for conservation values within marine parks.
A Case Study of Whistle Detection and Localization for Humpback Dolphins in Taiwan
Authors: Hung Ching-Tang , Chu Wei-Yen , Li Wei-Lun , Huang Yen-Hsiang , Hu Wei-Chun , Chen Chi-Fang
Published: June 2021

In recent years, Taiwan’s government has focused on policies regarding offshore wind farming near the Indo-Pacific humpback dolphin habitat, where marine mammal observation is a critical consideration. The present research developed an algorithm called National Taiwan University Passive Acoustic Monitoring (NTU_PAM) to assist marine mammal observers (MMOs). The algorithm performs whistle detection processing and whistle localization. Whistle detection processing is based on image processing and whistle feature extraction; whistle localization is based on the time difference of arrival (TDOA) method. To test the whistle detection performance, we used the same data to compare NTU_PAM and the widely used software PAMGuard. To test whistle localization, we designed a real field experiment where a sound source projected simulated whistles, which were then recorded by several hydrophone stations. The data were analyzed to locate the moving path of the source. The results show that localization accuracy was higher when the sound source position was in the detection region composed of hydrophone stations. This paper provides a method for MMOs to conveniently observe the migration path and population dynamics of cetaceans without ecological disturbance.
Study on Mechanical Bearing Strength and Failure Modes of Composite Materials for Marine Structures
Authors: Kim Dong-Uk , Seo Hyoung-Seock , Jang Ho-Yun
Published: June 2021

With the gradual application of composite materials to ships and offshore structures, the structural strength of composites that can replace steel should be explored. In this study, the mechanical bearing strength and failure modes of a composite-to-metal joining structure connected by mechanically fastened joints were experimentally analyzed. The effects of the fiber tensile strength and stress concentration on the static bearing strength and failure modes of the composite structures were investigated. For the experiment, quasi-isotropic [45°/0°/–45°/90°]2S carbon fiber-reinforced plastic (CFRP) and glass fiber-reinforced plastic (GFRP) specimens were prepared with hole diameters of 5, 6, 8, and 10 mm. The experimental results showed that the average static bearing strength of the CFRP specimen was 30% or higher than that of the GFRP specimen. In terms of the failure mode of the mechanically fastened joint, a cleavage failure mode was observed in the GFRP specimen for hole diameters of 5 mm and 6 mm, whereas a net-tension failure mode was observed for hole diameters of 8 mm and 10 mm. Bearing failure occurred in the CFRP specimens.
Distribution and Genetic Diversity of the Amphibian Chytrid in Japan
Authors: Goka Koichi , Yokoyama Jun , Tominaga Atsushi
Published: June 2021

While research on frog chytrid fungus Batrachochytrium dendrobatidis (Bd), an infectious disease that threatens amphibian diversity, continues to advance worldwide, little progress has been made in Japan since around 2010. The reason for this is, which we pointed out in 2009, that the origin of frog chytrid fungus may be in the East Asian region, including Japan based on the Bd ITS-DNA variation, and as few cases of mass mortality caused by this fungus have been observed in wild amphibian populations in Japan, the interest of the Japanese government and the general public in Bd has waned. However, we believe that organizing the data obtained so far in Japan and distributing the status of frog chytrid fungus in Japan to the world will provide useful insight for future risk management of this pathogen. We collected more than 5500 swab samples from wild amphibians throughout Japan from 2009 to 2010. Then, we investigated the infection status using the Nested-PCR method. We sequenced the obtained DNA samples and constructed a maximum-parsimony (MP) tree to clarify the phylogenetic diversity of Bd. We detected Bd infection in 11 (nine native and two alien) amphibian species in Japan and obtained 44 haplotypes of Bd ITS-DNA. The MP tree showed a high diversity of Bd strains in Japan, suggesting that some strains belong to Bd-GPL and Bd-Brazil. Except for local populations of the Japanese giant salamanders Andrias japonicus in Honshu Island and the sword tail newts Cynops ensicauda in Okinawa Island, the Bd infection prevalence in native amphibian species was very low. The alien bullfrog Aquarana catesbeiana had high Bd infection rates in all areas where they were sampled. No Bd infection was detected in other native amphibians in the areas where giant salamanders, sword tail newts, and bullfrogs were collected, suggesting that many native amphibians are resistant to Bd infection. The sword tail newt of Okinawa Island had both the highest infectious incidence and greatest number of haplotypes. The giant salamanders also showed relatively high infection prevalence, but the infected strains were limited to those specific to this species. These two Caudata species are endemic to a limited area of Japan, and it was thought that they may have been refugia for Bd, which had been distributed in Japan Islands for a long time.
Histoplasma capsulatum Isolated from Tadarida brasiliensis Bats Captured in Mexico Form a Sister Group to North American Class 2 Clade
Authors: Vite-Garín Tania , Estrada-Bárcenas Daniel A. , Gernandt David S. , Reyes-Montes María del Rocío , Sahaza Jorge H. , Canteros Cristina E. , Ramírez José A. , Rodríguez-Arellanes Gabriela , Serra-Damasceno Lisandra , Zancopé-Oliveira Rosely M. , Taylor John W. , Taylor Maria Lucia
Published: June 2021

Histoplasma capsulatum is a dimorphic fungus associated with respiratory and systemic infections in mammalian hosts that have inhaled infective mycelial propagules. A phylogenetic reconstruction of this pathogen, using partial sequences of arf, H-anti, ole1, and tub1 protein-coding genes, proposed that H. capsulatum has at least 11 phylogenetic species, highlighting a clade (BAC1) comprising three H. capsulatum isolates from infected bats captured in Mexico. Here, relationships for each individual locus and the concatenated coding regions of these genes were inferred using parsimony, maximum likelihood, and Bayesian inference methods. Coalescent-based analyses, a concatenated sequence-types (CSTs) network, and nucleotide diversities were also evaluated. The results suggest that six H. capsulatum isolates from the migratory bat Tadarida brasiliensis together with one isolate from a Mormoops megalophylla bat support a NAm 3 clade, replacing the formerly reported BAC1 clade. In addition, three H. capsulatum isolates from T. brasiliensis were classified as lineages. The concatenated sequence analyses and the CSTs network validate these findings, suggesting that NAm 3 is related to the North American class 2 clade and that both clades could share a recent common ancestor. Our results provide original information on the geographic distribution, genetic diversity, and host specificity of H. capsulatum.
Influence of CYP2C9 Genetic Polymorphisms on the Pharmacokinetics of Losartan and Its Active Metabolite E-3174: A Systematic Review and Meta-Analysis
Authors: Park Yoon-A , Song Yu-bin , Yee Jeong , Yoon Ha-Young , Gwak Hye-Sun
Published: June 2021

This study aimed to investigate the influence of CYP2C9 genetic polymorphisms on the pharmacokinetics of losartan and its active metabolite, E-3174, through a systematic review and meta-analysis. Eight studies published before March 2021 were included in this study. We used PubMed, the Cochrane Library, EMBASE, and Web of Science, based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The data analysis was conducted through Review Manager (RevMan), version 5.3, and R software. We found that healthy volunteers with CYP2C9*2 or *3 carriers had higher area under the curve (AUC0-∞) of losartan (mean difference (MD) 0.17 μg·h/mL; 95% confidence intervals (CI): 0.04, 0.29) and lower AUC0-∞ of E-3174 (MD −0.35 μg·h/mL; 95% CI: −0.62, −0.08) than those with CYP2C9*1/*1. Subjects with CYP2C9*2 or *3 carriers showed lower maximum concentration (Cmax) of E-3174 than those with CYP2C9*1/*1 (MD −0.13 μg/mL; 95% CI: −0.17, −0.09). For half-life, subjects with CYP2C9*2 or *3 carriers had longer half-lives of losartan and E-3174 than those with CYP2C9*1/*1 (MD 0.47 h; 95% CI: 0.32, 0.61 and MD 0.68 h; 95% CI: 0.44, 0.92, respectively). This meta-analysis suggests that the pharmacokinetics of losartan and E-3174 are associated with the CYP2C9 polymorphisms
The Signal Transducer IL6ST (gp130) as a Predictive and Prognostic Biomarker in Breast Cancer
Authors: Martínez-Pérez Carlos , Leung Jess , Kay Charlene , Meehan James , Gray Mark , Dixon J Michael , Turnbull Arran K
Published: June 2021

Novel biomarkers are needed to continue to improve breast cancer clinical management and outcome. IL6-like cytokines, whose pleiotropic functions include roles in many hallmarks of malignancy, rely on the signal transducer IL6ST (gp130) for all their signalling. To date, 10 separate independent studies based on the analysis of clinical breast cancer samples have identified IL6ST as a predictor. Consistent findings suggest that IL6ST is a positive prognostic factor and is associated with ER status. Interestingly, these studies include 4 multigene signatures (EndoPredict, EER4, IRSN-23 and 42GC) that incorporate IL6ST to predict risk of recurrence or outcome from endocrine or chemotherapy. Here we review the existing evidence on the promising predictive and prognostic value of IL6ST. We also discuss how this potential could be further translated into clinical practice beyond the EndoPredict tool, which is already available in the clinic. The most promising route to further exploit IL6ST’s promising predicting power will likely be through additional hybrid multifactor signatures that allow for more robust stratification of ER+ breast tumours into discrete groups with distinct outcomes, thus enabling greater refinement of the treatment-selection process.
Moving towards Integrated and Personalized Care in Parkinson’s Disease: A Framework Proposal for Training Parkinson Nurses
Authors: van Munster Marlena , Stümpel Johanne , Thieken Franziska , Pedrosa David , Antonini Angelo , Côté Diane , Fabbri Margherita , Ferreira Joaquim , Růžička Evžen , Grimes David , Mestre Tiago
Published: June 2021

Delivering healthcare to people living with Parkinson’s disease (PD) may be challenging in face of differentiated care needs during a PD journey and a growing complexity. In this regard, integrative care models may foster flexible solutions on patients’ care needs whereas Parkinson Nurses (PN) may be pivotal facilitators. However, at present hardly any training opportunities tailored to the care priorities of PD-patients are to be found for nurses. Following a conceptual approach, this article aims at setting a framework for training PN by reviewing existing literature on care priorities for PD. As a result, six prerequisites were formulated concerning a framework for training PN. The proposed training framework consist of three modules covering topics of PD: (i) comprehensive care, (ii) self-management support and (iii) health coaching. A fourth module on telemedicine may be added if applicable. The framework streamlines important theoretical concepts of professional PD management and may enable the development of novel, personalized care approaches.
PSMA Expression in 122 Treatment Naive Glioma Patients Related to Tumor Metabolism in 11C-Methionine PET and Survival
Authors: Traub-Weidinger Tatjana , Poetsch Nina , Woehrer Adelheid , Klebermass Eva-Maria , Bachnik Tatjana , Preusser Matthias , Mischkulnig Mario , Kiesel Barbara , Widhalm Georg , Mitterhauser Markus , Hacker Marcus , Koperek Oskar
Published: June 2021

Apart from its expression in benign and malignant prostate tissue, prostate specific membrane antigen (PSMA) was shown to be expressed specifically in the neovasculature of solid tumors. For gliomas only little information exists. Therefore, we aimed to correlate PSMA expression in gliomas to tumor metabolism by L-[S-methyl-11C]methionine (MET) PET and survival. Therefore, immunohistochemical staining (IHC) for isocitrate dehydrogenase 1-R132H (IDH1-R132H) mutation and PSMA expression was performed on the paraffin embedded tissue samples of 122 treatment-naive glioma patients. The IHC results were then related to the pre-therapeutic semiquantitative MET PET data and patients’ survival. Vascular PSMA expression was observed in 26 of 122 samples and was rather specific for high-grade gliomas ([HGG] 81% of glioblastoma multiforme, 10% of WHO grade III and just 2% of grade II gliomas). Significantly higher amounts of gliomas without verifiable IDH1-R132H mutation showed vascular PSMA expression. Significantly shorter median survival times were seen for patients with vascular PSMA staining in all tumors as well as HGG only. Additionally, significantly higher numbers of PSMA staining vessels were found in tumors with high amino acid metabolic rates. Vascular PSMA expression in gliomas was seen as a high-grade specific feature associated with elevated amino acid metabolism and short survival.
Exploring the Oral Microbiome in Rheumatic Diseases, State of Art and Future Prospective in Personalized Medicine with an AI Approach
Authors: Bellando-Randone Silvia , Russo Edda , Venerito Vincenzo , Matucci-Cerinic Marco , Iannone Florenzo , Tangaro Sabina , Amedei Amedeo
Published: June 2021

The oral microbiome is receiving growing interest from the scientific community, as the mouth is the gateway for numerous potential etiopathogenetic factors in different diseases. In addition, the progression of niches from the mouth to the gut, defined as “oral–gut microbiome axis”, affects several pathologies, as rheumatic diseases. Notably, rheumatic disorders (RDs) are conditions causing chronic, often intermittent pain affecting the joints or connective tissue. In this review, we examine evidence which supports a role for the oral microbiome in the etiology and progression of various RDs, including rheumatoid arthritis (RA), Sjogren’s syndrome (SS), and systemic lupus erythematosus (SLE). In addition, we address the most recent studies endorsing the oral microbiome as promising diagnostic biomarkers for RDs. Lastly, we introduce the concepts of artificial intelligence (AI), in particular, machine learning (ML) and their general application for understanding the link between oral microbiota and rheumatic diseases, speculating the application of a possible AI approach-based that can be applied to personalized medicine in the future.
When the Past Lives in the Present. Agrarian Landscapes and Historical Social Dynamics in the Southern Andes (Quebrada de Humahuaca, Jujuy, Argentina)
Authors: Cruz Pablo , Egan Nancy , Joffre Richard , Cladera Jorge L. , Winkel Thierry
Published: June 2021

This article examines the agrarian landscape in one part of the southern Andes (Quebrada of Humahuaca, Jujuy, Argentina). The region possesses extensive and well-preserved archaeological remains of agricultural systems, which stretch back to pre-Hispanic times. In this study, we employ an interdisciplinary approach in our analysis of the components that structure the agrarian landscape, especially those historical processes that intervened in its formation. The creation of a cartographic base, built from remote sensing and fieldwork data, allowed for the identification of four principal components of the landscape, each of which correspond to distinct phases or periods that mark the region’s history. Our study shows that, in contrast to what is observed in many other rural areas, the successive productive dynamics that developed in the area did not result in the destruction of previous productive structures. Rather, the agrarian landscape in the study area presents a multi-temporal agglutinating combination or composition, which transcends historical discontinuities in the productive matrix. This is owing to the partial reutilisation of previous structures in each period; however, religious and cultural factors play an important role. The agrarian landscape we studied is not only a passive result of human activity, but also a force influencing the productive and lifestyle decisions of the peasant populations that live there today. Our research amplifies the understanding of agrarian landscapes in the Andes and shows how past temporalities are articulated with the present through a dialectical process.
Sustainable Landscapes in the Traditional Korean Residential Environment: Focus on the Joseon Dynasty
Authors: Kim Hyung-Suk , Kim Kyu-Won
Published: June 2021

This study examines the sustainable landscapes displayed in traditional Korean houses of the Joseon Dynasty (1392–1910) by investigating urban agricultural practices implemented in the palace, private houses, and an imaginary garden (Uiwon). Currently popular productive gardening applications, such as urban agriculture, productive landscapes, and edible landscapes, also formed the backbone of the traditional Korean housing environment in the Joseon Dynasty, which has had substantial implications for today’s landscaping practices. Landscapes that are productive rather than decorative are important for environmentally sound and sustainable development not only for South Korea but also for modern housing design worldwide. This research provides concrete discussions of the definition and range of urban agriculture, agricultural methods, and principal agents of gardening-related businesses. This information advances the cultural conversation as well as the understanding and application of gardening in the modern context. Through a comparison of traditional and modern South Korean housing environments, this study contributes to developing the research on the role of gardens in sustainable landscapes.
Spatial Differentiation and Driving Factor Analysis of Urban Construction Land Change in County-Level City of Guangxi, China
Authors: Ouyang Dong , Zhu Xigang , Liu Xingguang , He Renfei , Wan Qian
Published: June 2021

The change of urban construction land is most obvious and intuitive in the change of global land use in the new era. The supply and allocation of construction land is an important policy tool for the government to carry out macro-control and spatial governance, which has received widespread attention from political circles, academia, and the public. An empirical study on the change of construction land and its driving factors in 70 county-level cities in Guangxi, China based on the GeoDetector method reveals the driving mechanism of the construction land change in county-level cities and provides more detailed information and a more accurate basis for county-level city policy makers and decision makers. The study shows a significant heterogeneity in the action intensity and interaction between construction land change and its driving factors in county-level cities, where population and GDP size, transportation, and industrial structure are determining factors. Besides, the factors of fiscal revenue, social consumption, utility investment, and real economy have a very weak action force individually, but they can achieve significant synergistic enhancement effects when coupled with other factors. In the end, urban construction land change at different scales and their driving mechanisms are somewhat different, and it is recommended to design differentiated and precise construction land control and spatial governance policies according to local conditions.
Metal-Organic Frameworks (MOFs)-Based Nanomaterials for Drug Delivery
Authors: Saeb Mohammad Reza , Rabiee Navid , Mozafari Masoud , Mostafavi Ebrahim
Published: June 2021

The composition and topology of metal-organic frameworks (MOFs) are exceptionally tailorable; moreover, they are extremely porous and represent an excellent Brunauer–Emmett–Teller (BET) surface area (≈3000–6000 m2·g−1). Nanoscale MOFs (NMOFs), as cargo nanocarriers, have increasingly attracted the attention of scientists and biotechnologists during the past decade, in parallel with the evolution in the use of porous nanomaterials in biomedicine. Compared to other nanoparticle-based delivery systems, such as porous nanosilica, nanomicelles, and dendrimer-encapsulated nanoparticles, NMOFs are more flexible, have a higher biodegradability potential, and can be more easily functionalized to meet the required level of host–guest interactions, while preserving a larger and fully adjustable pore window in most cases. Due to these unique properties, NMOFs have the potential to carry anticancer cargos. In contrast to almost all porous materials, MOFs can be synthesized in diverse morphologies, including spherical, ellipsoidal, cubic, hexagonal, and octahedral, which facilitates the acceptance of various drugs and genes.
Mesoporous Silica-Coated Upconverting Nanorods for Singlet Oxygen Generation: Synthesis and Performance
Authors: Zhang Zhen , Zhang Xiao-Lian , Li Bin
Published: June 2021

Photodynamic therapy (PDT) has been reported as a possible pathway for the treatment of tumors. The exploration for promising PDT systems thus attracts continuous research efforts. This work focused on an ordered core–shell structure encapsulated by mesoporous SiO2 with the upconverting emission property following a surfactant-assisted sol–gel technique. The mesoporous silica shell possessed a high surface area-to-volume ratio and uniform distribution in pore size, favoring photosensitizer (rose bengal) loading. Simultaneously, upconverting nanocrystals were synthesized and used as the core. After modification via hydrophobic silica, the hydrophobic upconverting nanocrystals became hydrophilic ones. Under near-infrared (NIR) light irradiation, the nanomaterials exhibited strong green upconverting luminescence so that rose bengal could be excited to produce singlet oxygen. The photodynamic therapy (PDT) feature was evaluated using a 1O2 fluorescent indicator. It was found that this core–shell structure generates 1O2 efficiently. The novelty of this core–shell structure was the combination of upconverting nanocrystals with a mesoporous SiO2 shell so that photosensitizer rose bengal could be effectively adsorbed in the SiO2 shell and then excited by the upconverting core.