bluuebunny/crossref_metadata_2025 huggingface dataset:
Probability Assessment of the Mechanical and Low-Cycle Properties of Structural Steels and Aluminium
Authors: Bazaras Žilvinas , Lukoševičius Vaidas , Vilkauskas Andrius , Česnavičius Ramūnas
Published: June 2021

Key mechanical properties used in low-cycle strength and durability calculations are the strength (proportional limit stress, σpr; relative yield strength, σ0.2; and ultimate tensile stress, σu) and strain properties (proportional limit strain, epr; percent area reduction, ψ; and percent area reduction at failure, ψu). When selecting the key mechanical properties provided in the specifications, an error may be made due to the failure to account for a series of random factors that determine the distribution of properties. The majority of research papers dealing with statistical descriptions of the low-cycle strain properties do not look deeper into the distribution of mechanical properties and the diagram parameters of strain characteristics. This paper provides a description of the distribution patterns of mechanical properties, statistical parameters, and low-cycle fatigue curves. Log-normal distribution generated the lowest values for the coefficient of variation of one of the key statistical indicators, suggesting that log-normal distribution is superior to normal or Weibull distribution in this respect. The distribution of low-cycle strain parameters exceeded the distribution of mechanical properties considerably. Minimum coefficients of variation of the parameters were generated at normal distribution. The statistical analysis showed the lower distribution of the durability parameters compared to the distribution of parameters of the strain diagrams. The findings of the paper enable a revision of the durability and life of the structural elements of in-service facilities subject to elastoplastic loading by assessing the distribution of mechanical characteristics and low-cycle strain parameters as well as the permissible distribution limits.
Temperature Dependent Stress–Strain Behavior and Martensite Stabilization in Magnetic Shape Memory Ni51.1Fe16.4Ga26.3Co6.2 Single Crystal
Authors: Lázpita Patricia , Villa Elena , Villa Francesca , Chernenko Volodymyr
Published: June 2021

The superelastic properties and stress-induced martensite (SIM) stabilization have been studied in a shape memory Ni51.1Fe16.4Ga26.3Co6.2 single crystal. The single crystal, characterized by a thermally induced forward martensitic transformation temperature around 56 °C in the initial state, has been submitted to compression mechanical testing at different temperatures well above, near and below the martensitic transformation (MT). After each mechanical test, the characteristic MT temperatures and the transformation enthalpy have been monitored by means of differential scanning calorimetry. At temperatures below MT, the stress–strain (σ–ε) curves show a large strain, around 6.0%, resulting from the detwinning process in the martensitic microstructure, which remains accumulated after unloading in the detwinned state of the sample as a typical behavior of the shape memory alloys (SMAs). After just two “σ–ε + heating” cycles the accumulation of strain was not observed any more indicating the formation of a two-way shape memory effect which consists in a spontaneous recovery of the aforementioned detwinned state of the sample during its cooling across the forward MT. Whereas the thermally induced shape recovery in conventional SMAs occurs at the fixed value of the reverse MT temperature, the heating DSC curves of the mechanically deformed martensite in the present work show a burst-like calorimetric peak at the reverse MT arising at temperatures essentially higher than the thermally activated one. This behavior is the result of the SIM stabilization effect. After a short thermal aging in the stress-free state, this effect almost disappears, showing a slight impact on the MT characteristic temperatures and the enthalpy. At temperatures higher than the transformation one, the SIM is not stabilized, as the mechanically induced martensite fully retransforms into austenite after the unloading. From the σ–ε curves, the critical stress, σc, as well as the values of Young’s moduli of martensite and austenite are determined showing linear dependences on the temperature with a slope of 3.6 MPa/°C.
Effects of Magnesium Oxide and Magnesium Hydroxide Microparticle Foliar Treatment on Tomato PR Gene Expression and Leaf Microbiome
Authors: Andreadelli Aggeliki , Petrakis Spyros , Tsoureki Antiopi , Tsiolas George , Michailidou Sofia , Baltzopoulou Penelope , Merkestein Robert van , Hodgson Philip , Sceats Mark , Karagiannakis George , Makris Antonios M.
Published: June 2021

Recently, metal oxides and magnesium hydroxide nanoparticles (NPs) with high surface-to-volume ratios were shown to possess antibacterial properties with applications in biomedicine and agriculture. To assess recent observations from field trials on tomatoes showing resistance to pathogen attacks, porous micron-scale particles composed of nano-grains of MgO were hydrated and sprayed on the leaves of healthy tomato (Solanum lycopersicum) plants in a 20-day program. The results showed that the spray induced (a) a modest and selective stress gene response that was consistent with the absence of phytotoxicity and the production of salicylic acid as a signalling response to pathogens; (b) a shift of the phylloplane microbiota from near 100% dominance by Gram (−) bacteria, leaving extremophiles and cyanobacteria to cover the void; and (c) a response of the fungal leaf phylloplane that showed that the leaf epiphytome was unchanged but the fungal load was reduced by about 70%. The direct microbiome changes together with the low level priming of the plant’s immune system may explain the previously observed resistance to pathogen assaults in field tomato plants sprayed with the same hydrated porous micron-scale particles.
Tectonic Evolution of the SE West Siberian Basin (Russia): Evidence from Apatite Fission Track Thermochronology of Its Exposed Crystalline Basement
Authors: Vetrov Evgeny V. , De Grave Johan , Vetrova Natalia I. , Zhimulev Fedor I. , Nachtergaele Simon , Van Ranst Gerben , Mikhailova Polina I.
Published: June 2021

The West Siberian Basin (WSB) is one of the largest intracratonic Meso-Cenozoic basins in the world. Its evolution has been studied over the recent decades; however, some fundamental questions regarding the tectonic evolution of the WSB remain unresolved or unconfirmed by analytical data. A complete understanding of the evolution of the WSB during the Mesozoic and Cenozoic eras requires insights into the cooling history of the basement rocks as determined by low-temperature thermochronometry. We presented an apatite fission track (AFT) thermochronology study on the exposed parts of the WSB basement in order to distinguish tectonic activation episodes in an absolute timeframe. AFT dating of thirteen basement samples mainly yielded Cretaceous cooling ages and mean track lengths varied between 12.8 and 14.5 μm. Thermal history modeling based on the AFT data demonstrates several Mesozoic and Cenozoic intracontinental tectonic reactivation episodes affected the WSB basement. We interpreted the episodes of tectonic activity accompanied by the WSB basement exhumation as a far-field effect from tectonic processes acting on the southern and eastern boundaries of Eurasia during the Mesozoic–Cenozoic eras.
Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant
Authors: Cañadas Olga , García-García Andrea , Prieto M. , Pérez-Gil Jesús
Published: June 2021

Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.
Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles
Authors: Korolev Dmitry , Shumilo Michael , Shulmeyster Galina , Krutikov Alexander , Golovkin Alexey , Mishanin Alexander , Gorshkov Andrew , Spiridonova Anna , Domorad Anna , Krasichkov Alexander , Galagudza Michael
Published: June 2021

In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action.
High-Efficiency Spin-Related Vortex Metalenses
Authors: Wang Wei , Zhao Ruikang , Chang Shilong , Li Jing , Shi Yan , Liu Xiangmin , Sun Jinghua , Kang Qianlong , Guo Kai , Guo Zhongyi
Published: June 2021

In this paper, one spin-selected vortex metalens composed of silicon nanobricks is designed and numerically investigated at the mid-infrared band, which can produce vortex beams with different topological charges and achieve different spin lights simultaneously. Another type of spin-independent vortex metalens is also designed, which can focus the vortex beams with the same topological charge at the same position for different spin lights, respectively. Both of the two vortex metalenses can achieve high-efficiency focusing for different spin lights. In addition, the spin-to-orbital angular momentum conversion through the vortex metalens is also discussed in detail. Our work facilitates the establishment of high-efficiency spin-related integrated devices, which is significant for the development of vortex optics and spin optics.
Oligomeric Formulas in Surgery: A Delphi and Consensus Study
Authors: De Luis Román Daniel , Domínguez Medina Eduardo , Molina Baena Begoña , Matía-Martín Pilar
Published: June 2021

Nutritional management of patients with intestinal failure often includes the use of oligomeric formulas. Implementing the use of oligomeric formulas in surgical patients with maldigestion or malabsorption could be a nutritional strategy to be included in clinical protocols. We aim to generate knowledge from a survey focused on the effectiveness of nutritional therapy with oligomeric formulas with Delphi methodology. Each statement that reached an agreement consensus among participants was defined as a median consensus score ≥7 and as an interquartile range ≤3. The use of oligomeric formulas in surgical patients, starting enteral nutrition in the post-operative phase in short bowel syndrome and in nonspecific diarrhea after surgical procedures, could improve nutritional therapy implementation. Stakeholders agreed that early jejunal enteral nutrition with oligomeric formula is more effective compared to intravenous fluid therapy and it is useful in patients undergoing upper gastro-intestinal tract major surgery when malabsorption or maldigestion is suspected. Finally, oligomeric formulas may be useful when a feeding tube is placed distally to the duodenum. This study shows a practical approach to the use of oligomeric formulas in surgical patients with intestinal disorders and malabsorption, and it helps clinicians in the decision-making process.
Changes in Lifestyle and Dietary Habits during COVID-19 Lockdown in Italy: Results of an Online Survey
Authors: Prete Melania , Luzzetti Anna , Augustin Livia S. A. , Porciello Giuseppe , Montagnese Concetta , Calabrese Ilaria , Ballarin Giada , Coluccia Sergio , Patel Linia , Vitale Sara , Palumbo Elvira , Celentano Egidio , La Vecchia Carlo , Crispo Anna
Published: June 2021

COVID-19 is an unprecedented global pandemic. On 12 March 2020, a lockdown order was issued in Italy in attempt to contain the health crisis. The study aimed to assess the impact of the COVID-19 lockdown on diet, physical activity, sleep quality, and distress in an Italian cohort. An online anonymous interview, which included validated questionnaires was created to compare lifestyle habits pre- and during the lockdown. Data analysis from 604 subjects with a mean age of 29.8 years was carried out using multivariate analysis. Compared to pre-COVID-19 times, 67% of people changed their eating habits and increased consumption of foods containing added sugars. Women and men with low adherence to the Mediterranean Diet (MedDiet) were more likely to be physically inactive (p < 0.0001 and p < 0.01, respectively). Results from logistic regression showed a three times higher risk of being inactive if adherence to the MedDiet was low (p < 0.0001), especially in men between 26 and 35 years. Lower levels of distress were reported in males who were physically active (89%) (p < 0.001). Our findings may help to identify effective lifestyle interventions during restrictive conditions.
The Effect of Religious Dietary Cultures on Food Nitrogen and Phosphorus Footprints: A Case Study of India
Authors: Dhar Aurup Ratan , Oita Azusa , Matsubae Kazuyo
Published: June 2021

The excessive consumption of nitrogen (N) and phosphorus (P), two vital nutrients for living organisms, is associated with negative environmental and health impacts. While food production contributes to a large amount of N and P loss to the environment, very little N and P is consumed as food. Food habits are affected by multiple regulations, including the dietary restrictions and dictates of various religions. In this study, religion-sensitive N-Calculator and P-Calculator approaches were used to determine the impact of religious dietary culture on the food N and P footprints of India in the major religious communities. Using 2013 data, the food N footprint of Hindus, Muslims, Christians, and Buddhists was 10.70, 11.45, 11.47, and 7.39 kg-N capita−1 year−1 (10.82 kg-N capita−1 year−1 was the national average), and the food P footprint was 1.46, 1.58, 1.04. and 1.58 kg-P capita−1 year−1 (1.48 kg-P capita−1 year−1 was the national average). The findings highlight the impact of individual choice on the N and P food footprints, and the importance of encouraging the followers of religion to follow a diet consistent with the food culture of that religion. The results of this study are a clear indication of the requirement for religion-sensitive analyses in the collecting of data pertinent to a particular country for use in making government policies designed to improve the recycling of food waste and the treatment of wastewater.
Progress towards Achieving the Recommendations of the Commission on Ending Childhood Obesity: A Comprehensive Review and Analysis of Current Policies, Actions and Implementation Gaps in Thailand
Authors: Phulkerd Sirinya , Nakraksa Parichat , Mo-suwan Ladda , Lawrence Mark
Published: June 2021

Despite a significant commitment to tackling childhood overweight and obesity, questions remain about the progress the Thai Government has made in implementing childhood obesity prevention policies and actions. This study aimed to review and assess the implementation of the government’s policies and actions for childhood obesity prevention in Thailand compared with the recommendations of the Commission on Ending Childhood Obesity and to identify the implementation gaps. Policy data were collected from governmental and NGO websites and publications and via direct contact with government officials. Stakeholder meetings were held to seek further information and advice on implementation gaps and to give recommendations. The analysis of each policy was conducted against pre-determined criteria formulated from literature assessments and stakeholder consultations. The policies and actions that were implemented by the Government were consistent with 33 broad policy actions and 55 specific policy actions. Preconception and pregnancy care was the policy area that was most implemented. Six broad policy actions were assessed as ‘high’ performance, these were: sugar-sweetened beverage taxation, nutrient labeling, nutrition guidance for preconception and pregnancy care, the International Code of Marketing of Breast-milk Substitutes, regulatory measures for supporting maternal breastfeeding, and regulations on the marketing of complementary foods and beverages. Policy coherence and monitoring and evaluation (M&E) were identified as major implementation gaps. Increasing the effectiveness of childhood obesity prevention in Thailand will require national immediate attention towards building infrastructure to enhance coherence among the policies and to put in place M&E mechanisms for each policy.
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors
Authors: Queiroz Alice L. N. , Barros Rafael S. , Silva Sandro P. , Rodrigues Daniela S. G. , Cruz Ana C. R. , dos Santos Flávia B. , Vasconcelos Pedro F. C. , Tesh Robert B. , Nunes Bruno T. D. , Medeiros Daniele B. A.
Published: June 2021

From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a naïve population brought another concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR (RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI) by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV genotypes and geographic strains that circulate in Central and South America. Moreover, after testing around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast region, showing lower incidence than AEFI as expected.
High Rates of Hidden HCV Infections among Hospitalized Patients Aged 55–85
Authors: Piazzolla Annarita Valeria , Paroni Giulia , Bazzocchi Francesca , Cassese Mauro , Cisternino Antonio , Ciuffreda Luigi , Gorgoglione Franco , Gorgoglione Leonardo , Palazzo Vincenzo , Sciannamè Natale , Taurchini Marco , Vaira Pasquale , Cocomazzi Giovanna , Squillante Maria Maddalena , Aucella Filippo , Cascavilla Nicola , De Cosmo Salvatore , Fania Michelantonio , Greco Antonio , Laborante Antonio , Leone Maurizio , Maiello Evaristo , Salvatori Mauro , Di Mauro Lazzaro , Mangia Alessandra
Published: June 2021

Background and Aims: The WHO has solicited all countries to eliminate HCV by 2030. The Italian government started routine screening for HCV infection in January 2021, initially targeting subjects born between 1969 and 1989. With the aim of achieving micro-elimination, we designed a hospital-wide project focusing on inpatients born from 1935 to 1985 and conducted it in our institution. Method: All inpatients aged 35 to 85, admitted from 10 February 2020 to 9 February 2021 for many different diseases and conditions underwent HCV antibody (HCVAb) testing by third-generation ELISA. When positive, reflex HCV RNA testing and genotyping were performed. Clinical history, fibrosis diagnosis, laboratory data and concomitant medications were available for all. Results: The HCV screening rate of inpatients was 100%. In total, 11,748 participants were enrolled, of whom 53.50% were male. The HCVAb positivity rate was 3.03%. The HCVAb rate increased with age and was higher for patients born between 1935 and 1944 (4.81%). The rate of HCV RNA positivity was 0.97%. The vast majority (80.70%) of HCV RNA-positive participants were 55 or older; in about 40% of cases, HCV RNA-positive patients were unaware of their infection. Although 16 patients died after HCV chronic infection diagnosis (two due COVID-19) or HCV treatment prescription (one due to COVID-19), 74.56% of patient HCV diagnoses were linked to HCV treatment, despite their co-morbidities. All patients older than 65 who died had an active HCV infection. Conclusion: The present study revealed a rate of active HCV infections among inpatients lower than what has been reported in the past in the general population; this appears to be a result of the widespread use of pangenotypic direct-acting antiviral agents (DAAs). The overall rate of active infection was lower than the rate observed in the 1935–1954 cohort. The high rate of inpatients unaware of HCV infections and the high number of deaths among subjects with an active HCV infection born from 1935 to 1954, suggest that, at least in southern Italy, targeted screening of this birth cohort may be required to reduce the number of undiagnosed cases and hidden infections.
Sow Contact Is a Major Driver in the Development of the Nasal Microbiota of Piglets
Authors: Obregon-Gutierrez Pau , Aragon Virginia , Correa-Fiz Florencia
Published: June 2021

The nasal microbiota composition is associated with the health status of piglets. Sow-contact in early life is one of the factors influencing the microbial composition in piglets; however, its impact has never been assessed in the nasal microbiota of piglets reared in controlled environmental conditions. Nasal microbiota of weaning piglets in high-biosecurity facilities with different time of contact with their sows (no contact after farrowing, contact limited to few hours or normal contact until weaning at three weeks) was unveiled by 16S rRNA gene sequencing. Contact with sows demonstrated to be a major factor affecting the nasal microbial composition of the piglets. The nasal microbiota of piglets that had contact with sows until weaning, but were reared in high biosecurity facilities, was richer and more similar to the previously described healthy nasal microbiota from conventional farm piglets. On the other hand, the nasal communities inhabiting piglets with no or limited contact with sows was different and dominated by bacteria not commonly abundant in this body site. Furthermore, the length of sow–piglet contact was also an important variable. In addition, the piglets raised in BSL3 conditions showed an increased richness of low-abundant species in the nasal microbiota. Artificially rearing in high biosecurity facilities without the contact of sows as a source of nasal colonizers had dramatic impacts on the nasal microbiota of weaning piglets and may introduce significant bias into animal research under these conditions.
Functional Connectivity between Task-Positive Networks and the Left Precuneus as a Biomarker of Response to Lamotrigine in Bipolar Depression: A Pilot Study
Authors: Martens Marieke , Filippini Nicola , Masaki Charles , Godlewska Beata R.
Published: June 2021

Treatment of bipolar depression poses a significant clinical challenge. Lamotrigine is one of a few efficacious drugs, however, it needs to be titrated very slowly and response can only be assessed after 10–12 weeks. With only a proportion of patients responding, an exploration of factors underlying treatment responsivity is of paramount clinical importance, as it may lead to an allocation of the drug to those most likely to respond to it. This study aimed at identifying differences in patterns of pre-treatment resting state functional connectivity (rsFC) that may underlie response to lamotrigine in bipolar depression. After a baseline MRI scan, twenty-one patients with bipolar depression were treated with lamotrigine in an open-label design; response, defined as ≥50% decrease in Hamilton Depression Rating Scale (HAMD) score, was assessed after 10–12 weeks of treatment. Twenty healthy controls had a baseline clinical assessment and scan but did not receive any treatment. Fifteen out of 21 (71%) patients responded to lamotrigine. Treatment responsivity was associated with enhanced pre-treatment rsFC of the right fronto-parietal network (FPN) and dorsal attention network (DAN) with left precuneus. The lack of treatment response was additionally characterised by reduced rsFC: of the DAN with right middle temporal gyrus; of the default mode network (DMN) with left precuneus; of the extended sensory-motor area with areas including the left hippocampus/left amygdala and left subcallosal cortex/nucleus accumbens; and of the left FPN with left inferior temporal gyrus/occipital fusiform gyrus/lateral occipital cortex. The results suggest that preserved rsFC between the FPN and DAN, the networks involved in cognitive control, and the hub of the posterior DMN, the left precuneus, may be critical for good response to lamotrigine as an add-on treatment in patients with bipolar depression. The study also suggests a more general decrease in rsFC to be related to poor treatment responsivity.
Prediction of Drug Stability Using Deep Learning Approach: Case Study of Esomeprazole 40 mg Freeze-Dried Powder for Solution
Authors: Ajdarić Jovana , Ibrić Svetlana , Pavlović Aleksandar , Ignjatović Ljubiša , Ivković Branka
Published: June 2021

A critical step in the production of Esomeprazole powder for solution is a period between the filling process and lyophilization, where all vials, partially closed, are completely exposed to environmental influences. Excessive instability reflects in pH value variations caused by oxygen’s impact. In order to provide pH control, which consequently affects drug stability, Esomeprazole batches, produced in the same way, were kept in partially closed vials for 3 h at temperatures of 20 °C and −30 °C, after which they were lyophilized and stored for long-term stability for 36 months. The aim of the presented study was to apply a deep-learning algorithm for the prediction of the Esomeprazole stability profile and to determine the pH limit for the reconstituted solution of the final freeze-dried product that would assure a quality product profile over a storage period of 36 months. Multilayer perceptron (MLP) as a deep learning tool, with four layers, was used. The pH value of Esomeprazole solution and time of storage (months) were inputs for the network, while Esomeprazole assay and four main impurities were outputs of the network. In order to keep all related substances and Esomeprazole assay in accordance with specifications for the whole shelf life, the pH value for the reconstituted finish product should be set in the range of 10.4–10.6.
The Role of PK/PD Analysis in the Development and Evaluation of Antimicrobials
Authors: Rodríguez-Gascón Alicia , Solinís María Ángeles , Isla Arantxa
Published: June 2021

Pharmacokinetic/pharmacodynamic (PK/PD) analysis has proved to be very useful to establish rational dosage regimens of antimicrobial agents in human and veterinary medicine. Actually, PK/PD studies are included in the European Medicines Agency (EMA) guidelines for the evaluation of medicinal products. The PK/PD approach implies the use of in vitro, ex vivo, and in vivo models, as well as mathematical models to describe the relationship between the kinetics and the dynamic to determine the optimal dosing regimens of antimicrobials, but also to establish susceptibility breakpoints, and prevention of resistance. The final goal is to optimize therapy in order to maximize efficacy and minimize side effects and emergence of resistance. In this review, we revise the PK/PD principles and the models to investigate the relationship between the PK and the PD of antibiotics. Additionally, we highlight the outstanding role of the PK/PD analysis at different levels, from the development and evaluation of new antibiotics to the optimization of the dosage regimens of currently available drugs, both for human and animal use.
Blurring the Boundaries between a Branch and a Flower: Potential Developmental Venues in CACTACEAE
Authors: Rosas-Reinhold Isaura , Piñeyro-Nelson Alma , Rosas Ulises , Arias Salvador
Published: June 2021

Flowers are defined as short shoots that carry reproductive organs. In Cactaceae, this term acquires another meaning, since the flower is interpreted as a branch with a perianth at the tip, with all reproductive organs embedded within the branch, thus giving way to a structure that has been called a “flower shoot”. These organs have long attracted the attention of botanists and cactologists; however, the understanding of the morphogenetic processes during the development of these structures is far from clear. In this review, we present and discuss some classic flower concepts used to define floral structures in Cactaceae in the context of current advances in flower developmental genetics and evolution. Finally, we propose several hypotheses to explain the origin of these floral shoot structures in cacti, and we suggest future research approaches and methods that could be used to fill the gaps in our knowledge regarding the ontogenetic origin of the “flower” in the cactus family.
Azospirillum baldaniorum Sp245 Induces Physiological Responses to Alleviate the Adverse Effects of Drought Stress in Purple Basil
Authors: Mariotti Lorenzo , Scartazza Andrea , Curadi Maurizio , Picciarelli Piero , Toffanin Annita
Published: June 2021

Azospirillum spp. are plant growth-promoting rhizobacteria (PGPR) that exert beneficial effects on plant growth and yield of agronomically important plant species. The aim of this study was to investigate the effects of a root treatment with Azospirillum baldaniorum Sp245 on hormones in xylem sap and physiological performance in purple basil (Ocimum basilicum L. cv. Red Rubin) plants grown under well-watered conditions and after removing water. Treatments with A. baldaniorum Sp245 included inoculation with viable cells (1·107 CFU mL–1) and addition of two doses of filtered culture supernatants (non-diluted 1·108 CFU mL–1, and diluted 1:1). Photosynthetic activity, endogenous level of hormones in xylem sap (salicylic acid, jasmonic acid, and abscisic acid), leaf pigments, leaf water potential, water-use efficiency (WUE), and drought tolerance were determined. Fluorescence and gas exchange parameters, as well as leaf water potential, showed that the highest dose of filtered culture supernatant improved both photosynthetic performance and leaf water status during water removal, associated with an increase in total pigments. Moreover, gas exchange analysis and carbon isotope discrimination found this bacterial treatment to be the most effective in inducing an increase of intrinsic and instantaneous WUE during water stress. We hypothesize that the benefits of bacterial treatments based on A. baldaniorum Sp245 are strongly correlated with the synthesis of phytohormones and the induction of plant-stress tolerance in purple basil.
A Slow-Release Fertilizer of Urea Prepared via Melt Blending with Degradable Poly(lactic acid): Formulation and Release Mechanisms
Authors: Kaavessina Mujtahid , Distantina Sperisa , Shohih Esa Nur
Published: June 2021

In this research, a low molecular weight poly(lactic acid) (or PLA) synthesized from direct polycondensation was melt compounded with urea to formulate slow-release fertilizer (SRF). We studied the influence of the molecular weight (MW) of PLA as a matrix and the urea composition of SRF towards release kinetics in water at 30 °C. The physical appearance of solid samples, the change in urea concentration, and acidity (pH) of water were monitored periodically during the release test. Three studied empirical models exhibited that diffusion within the matrix dominated the urea release process, especially when the release level was less than 60%. Thus, a lower MW of PLA and a higher urea content of SRF showed a faster release rate. For the entire length of the release experiment, a combination of diffusion and degradation mechanisms exhibited the best agreement with the experimental data. The hydrolytic degradation of PLA may begin after 96 h of immersion (around 60% release level), followed by the appearance of some micro-holes and cracks on the surface of the SRF samples. Generally, this research revealed the good release performance of urea without residues that damage the soil structure and nutrient balance.
Ultrafine PVDF Nanofibers for Filtration of Air-Borne Particulate Matters: A Comprehensive Review
Authors: Sanyal Ayishe , Sinha-Ray Sumit
Published: June 2021

The ongoing global pandemic has bestowed high priority uponthe separation of air-borne particulate matters (PMs), aerosols, etc. using nonwoven fibrous materials, especially for face masks as a means of personal protection. Although spunbond or meltblown nonwoven materials are amongst the forerunners for polymer microfiber-based face mask or air filter development in mass scale, relatively new process of nonwoven manufacturing such as electrospinning is gaining a lot of momentum amongst the filter membrane manufacturers for its scalability of nanofiber-based filter membrane fabrication. There are several nanofiber-based face masks developing industries, which claim a very high efficiency in filtration of particulate matters (PM0.1–10) as well as other aerosols for their products. Polyvinylidene fluoride (PVDF), which is commonly known for its use of tactile sensors and energy harvesters, due to its piezoelectric property, is slowly gaining popularity among researchers and developers as an air filter material. Electrospun PVDF nanofibers can be as fine as 50 nm in mass scale, which allows the membrane to have large surface area compared to its volume, enhancing nanofiber–PM interaction. At the same time, the breathability index can be improved through these PVDF nanofiber membranes due to their architectural uniqueness that promotes slip flow around the fibers. The conductive nature of PVDF makes it advantageous as a promising electret filter allowing better capturing of ultrafine particles. This review aims to provide a comprehensive overview of such PVDF nanofiber-based filter membranes and their roles in air filtration, especially its application in filtrate of air-borne PMs.
Can Decentralization Drive Green Innovation? A Game Theoretical Analysis of Manufacturer Encroachment Selection with Consumer Green Awareness
Authors: Cao Dan , Li Jin , Liu Gege , Mei Ran
Published: June 2021

With the increase of public environmental awareness and the growth of e-commerce, sustainable development promotes the manufacturer to increasingly participate in green innovation and make full use of the online sales channel to enhance competitiveness. Despite decentralized encroachment being widely adopted in business reality, the current literature has commonly paid more attention to centralized encroachment. To complement related research, a dual-channel green supply chain composed of a manufacturer (its retail subsidiary) and a retailer is investigated. We focus on what encroachment strategy (centralization vs. decentralization) drives the green innovation and analyze the impact of consumer green awareness and product substitutability on the manufacturer’s encroachment strategy, green innovation efforts and supply chain performance. Under each encroachment strategy, we build a Stackelberg game model and derive the equilibrium outcome. Then, we theoretically analyze the effects of consumer green awareness and product substitutability on green innovation and each party’s profitability. Our comparative analysis shows what encroachment strategy drives green innovation and what encroachment strategy benefits both parties and social welfare. Numerical studies are also conducted to support the analytical results. Our key findings reveal that decentralization improves the green innovation and achieves a both-win situation for the manufacturer and the retailer. Besides that, decentralization can reduce the environmental damage and increase social welfare as well.
Trier Social Stress Test Elevates Blood Pressure, Heart Rate, and Anxiety, But a Singing Test or Unsolvable Anagrams Only Elevates Heart Rate, among Healthy Young Adults
Authors: Sequeira Isabelle K. , Longmire Addie S. , McKay Naomi J.
Published: June 2021

The Trier Social Stress Test (TSST) is a psychosocial stressor that effectively stimulates the stress response but is labor and time intensive. Although other psychological stressors are often used experimentally, none are known to comparably elevate stress. Two stressors that may potentially elevate stress are a singing task (ST) and unsolvable anagrams, but there are not enough data to support their effectiveness. In the current experiment, 53 undergraduate males and females (mean age = 21.9 years) were brought into the laboratory, and baseline blood pressure, heart rate, self-rated anxiety, and salivary cortisol were recorded. Then, participants were randomly assigned to one of three stress conditions: TSST (n = 24), ST (n = 14), or an unsolvable anagram task (n = 15). Stress measures were taken again after the stressor and during recovery. The TSST significantly elevated systolic blood pressure, diastolic blood pressure, heart rate, and self-rated anxiety from pre-stress levels, replicating its stress-inducing properties. However, the ST and unsolvable anagrams only elevated heart rate, indicating that these methods are not as able to stimulate physiological or psychological stress. Overall, results indicate that out of these three laboratory stressors, the TSST clearly engages the stress response over the ST or unsolvable anagrams.
Desiring the Secular: Capital, Cohesion, and the Fantasy of Secularization
Authors: Morrison Ian A.
Published: June 2021

Towards the end of the twentieth century, religion re-emerged as a topic of pressing concern in a number of the most self-consciously secularized states of the global north. From disputes over the wearing of headscarves in schools to debates over accommodations for religious practices in the public sphere, religion, particularly the ‘foreign’ religiosity of migrants and other minority religious subjects, appeared on the scene as a phenomenon whose proper place and role in society required both urgent and careful deliberation. This article argues that in order to account for the affective potency produced by the immanence of the figure of the ‘foreign’ religious subject, it is necessary to understand secularization as fantasy. It is within the fantasy of secularization that the secular emerges as an object of desire—as something that, if attained, appears as a solution to the problem of ‘foreign’ religiosity—and figures of inassimilable religiosity assume the role of scapegoats for the failure to resolve these concerns. In this sense, within this fantasy scene, the secular promises to provide ‘us’ with something that we are lacking. However, this promise has been undermined by the apparent persistence of religious difference. As such, as a result of their continued religiosity, ‘they’ appear to be taking something from ‘us’.
Cascaded Microwave Frequency Transfer over 300-km Fiber Link with Instability at the 10−18 Level
Authors: Xue Wenxiang , Zhao Wenyu , Quan Honglei , Xing Yan , Zhang Shougang
Published: June 2021

Comparing and synchronizing atomic clocks between distant laboratories with ultra-stable frequency transfer are essential procedures in many fields of fundamental and applied science. Existing conventional methods for frequency transfer based on satellite links, however, are insufficient for the requirements of many applications. In order to achieve high-precision microwave frequency transfer over a thousand kilometers of fiber and to construct a fiber-based microwave transfer network, we propose a cascaded system for microwave frequency transfer consisting of three 100-km single-span spooled fiber links using an improved electronic phase compensation scheme. The transfer instability measured for the microwave signal reaches 1.1 × 10−14 at 1 s and 6.8 × 10−18 at 105 s, which agrees with the root-sum-square of each span contribution. It is feasible to extend the length of the fiber-based microwave frequency transfer up to 1200 km using 4 stages of our cascaded system, which is still sufficient to transfer modern cold atom microwave frequency standards. Moreover, the transfer instability of 9.0 × 10−15 at 1 s and 9.0 × 10−18 at 105 s for a 100-MHz signal is achieved. The residual phase noise power spectral density of the 300-km cascaded link measured at 100-MHz is also obtained. The rejection frequency bandwidth of the cascaded link is limited by the propagation delay of one single-span link.
The Precipitation Imaging Package: Phase Partitioning Capabilities
Authors: Pettersen Claire , Bliven Larry F. , Kulie Mark S. , Wood Norman B. , Shates Julia A. , Anderson Jaclyn , Mateling Marian E. , Petersen Walter A. , von Lerber Annakaisa , Wolff David B.
Published: June 2021

Surface precipitation phase is a fundamental meteorological property with immense importance. Accurate classification of phase from satellite remotely sensed observations is difficult. This study demonstrates the ability of the Precipitation Imaging Package (PIP), a ground-based, in situ precipitation imager, to distinguish precipitation phase. The PIP precipitation phase identification capabilities are compared to observer records from the National Weather Service (NWS) office in Marquette, Michigan, as well as co-located observations from profiling and scanning radars, disdrometer data, and surface meteorological measurements. Examined are 13 events with at least one precipitation phase transition. The PIP-determined onsets and endings of the respective precipitation phase periods agree to within 15 min of NWS observer records for the vast majority of the events. Additionally, the PIP and NWS liquid water equivalent accumulations for 12 of the 13 events were within 10%. Co-located observations from scanning and profiling radars, as well as reanalysis-derived synoptic and thermodynamic conditions, support the accuracy of the precipitation phases identified by the PIP. PIP observations for the phase transition events are compared to output from a parameterization based on wet bulb and near-surface lapse rates to produce a probability of solid precipitation. The PIP phase identification and the parameterization output are consistent. This work highlights the ability of the PIP to properly characterize hydrometeor phase and provide dependable precipitation accumulations under complicated mixed-phase and rain and snow (or vice versa) transition events.
Building Extraction from Very-High-Resolution Remote Sensing Images Using Semi-Supervised Semantic Edge Detection
Authors: Xia Liegang , Zhang Xiongbo , Zhang Junxia , Yang Haiping , Chen Tingting
Published: June 2021

The automated detection of buildings in remote sensing images enables understanding the distribution information of buildings, which is indispensable for many geographic and social applications, such as urban planning, change monitoring and population estimation. The performance of deep learning in images often depends on a large number of manually labeled samples, the production of which is time-consuming and expensive. Thus, this study focuses on reducing the number of labeled samples used and proposing a semi-supervised deep learning approach based on an edge detection network (SDLED), which is the first to introduce semi-supervised learning to the edge detection neural network for extracting building roof boundaries from high-resolution remote sensing images. This approach uses a small number of labeled samples and abundant unlabeled images for joint training. An expert-level semantic edge segmentation model is trained based on labeled samples, which guides unlabeled images to generate pseudo-labels automatically. The inaccurate label sets and manually labeled samples are used to update the semantic edge model together. Particularly, we modified the semantic segmentation network D-LinkNet to obtain high-quality pseudo-labels. Specifically, the main network architecture of D-LinkNet is retained while the multi-scale fusion is added in its second half to improve its performance on edge detection. The SDLED was tested on high-spatial-resolution remote sensing images taken from Google Earth. Results show that the SDLED performs better than the fully supervised method. Moreover, when the trained models were used to predict buildings in the neighboring counties, our approach was superior to the supervised way, with line IoU improvement of at least 6.47% and F1 score improvement of at least 7.49%.
Determination of Nicotine-Related Impurities in Nicotine Pouches and Tobacco-Containing Products by Liquid Chromatography–Tandem Mass Spectrometry
Authors: Avagyan Rozanna , Spasova Maya , Lindholm Johan
Published: June 2021

Smokeless tobacco products and nicotine-containing tobacco-free oral pouches have increased in popularity in recent years. They are associated with far fewer health hazards compared to cigarettes. Nicotine pouches are filled with non-tobacco filler and nicotine. The nicotine used in nicotine pouches usually comes from the extraction of tobacco; thus, related alkaloids may be found as impurities at low levels. Moreover, nicotine degradation products are formed because of microbial action, flavor oxidation, exposure to high temperatures etc. Currently, there are no published or recommended methods for the analysis of nicotine degradants in nicotine pouches. Here, we present a sensitive and selective liquid chromatography–tandem mass spectrometry method for the simultaneous determination of seven nicotine-related impurities. All seven analytes and corresponding deuterated internal standards were separated within 3.5 min, including 1 min equilibration. The method was fully validated, showing good linearity with correlation coefficients >0.996 for all analytes, good extraction yields ranging from 78% to 110%, limits of detection between 0.08 and 0.56 µg/g and limits of quantification between 0.27 and 2.04 µg/g. Although the method was mainly developed to determine the degradants of nicotine in nicotine pouches, it was validated and performed well on a broader range of tobacco-containing products.