bluuebunny/crossref_metadata_2025 huggingface dataset:
Sequential Nuclear Accumulation of the Clock Proteins Period and Timeless in the Pacemaker Neurons ofDrosophila melanogaster
Authors: Shafer Orie T. , Rosbash Michael , Truman James W.
Published: April 2018

Antisera against the circadian clock proteins Period (PER) and Timeless (TIM) were used to construct a detailed time course of PER and TIM expression and subcellular localization in a subset of the ventrolateral neurons (vLNs) in theDrosophilaaccessory medulla (AMe). These neurons, which express pigment-dispersing factor, play a central role in the control of behavioral rhythms. The data revealed several unexpected features of the circadian clock inDrosophila. First, TIM but not PER was restricted to the cytoplasm of vLNs throughout most of the early night. Second, the timing of TIM and PER nuclear accumulation was substantially different. Third, the two subsets of vLNs, the large and small vLNs, had a similar timing of PER nuclear accumulation but differed by 3–4 hr in the phase of TIM nuclear accumulation. These aspects of PER and TIM expression were not predicted by the current mechanistic model of the circadian clock inDrosophilaand are inconsistent with the hypothesis that PER and TIM function as obligate heterodimers. The differing profiles of TIM and PER nuclear accumulation suggest that PER and TIM have distinct functions in the nuclei of vLNs.
Mapping the Binding Site of the Neuroprotectant Ifenprodil on NMDA Receptors
Authors: Perin-Dureau Florent , Rachline Julie , Neyton Jacques , Paoletti Pierre
Published: April 2018

Ifenprodil is a noncompetitive antagonist of NMDA receptors highly selective for the NMDA receptor 2B (NR2B) subunit. It is widely used as a pharmacological tool to discriminate subpopulations of NMDA receptors, and derivatives are currently being developed as candidate neuroprotectants. Despite numerous studies on the mechanism of action of ifenprodil on NMDA receptors, the structural determinants responsible for the subunit selectivity have not been identified. By combining functional studies on recombinant NMDA receptors and biochemical studies on isolated domains, we now show that ifenprodil binds to the N-terminal leucine/isoleucine/valine-binding protein (LIVBP)-like domain of NR2B. In this domain, several residues, both hydrophilic and hydrophobic, were found to control ifenprodil inhibition. Their location in a modeled three-dimensional structure suggests that ifenprodil binds in the cleft of the LIVBP-like domain of NR2B by a mechanism (Venus-flytrap) resembling that of the binding of Zn on the LIVBP-like domain of NR2A. These results reinforce the proposal that the LIVBP-like domains of NMDA receptors, and possibly of other ionotropic glutamate receptors, bind modulatory ligands. Moreover, they identify the LIVBP-like domain of the NR2B subunit as a promising therapeutic target and provide a framework for designing structurally novel NR2B-selective antagonists.
Tyrosine Phosphorylation of the NR2B Subunit of the NMDA Receptor in the Spinal Cord during the Development and Maintenance of Inflammatory Hyperalgesia
Authors: Guo Wei , Zou Shiping , Guan Yun , Ikeda Tetsuya , Tal Michael , Dubner Ronald , Ren Ke
Published: April 2018

The present study examined the levels of NMDA receptor NR2 subunit tyrosine phosphorylation in a rat model of inflammation and correlated it with the development of inflammation and hyperalgesia. Hindpaw inflammation and hyperalgesia were induced by intraplantar injection of complete Freund's adjuvant. Proteins from the spinal cord (L4–L5) were immunoprecipitated with anti-NR2A or anti-NR2B antibodies and used for subsequent analysis using 4G-10, a specific anti-phosphotyrosine antibody. Compared with naive rats, there was a rapid and prolonged increase in tyrosine phosphorylation of the NR2B, but not NR2A, subunit after inflammation. The increase in NR2B tyrosine phosphorylation was dependent on primary afferent drive because (1) the phosphorylation correlated with the temporal profile of inflammation and hyperalgesia, (2) shorter-duration noxious stimulation produced a rapid and shorter-lasting increase in phosphorylation, and (3) local anesthetic block of the injected paw reversibly blocked inflammation-induced NR2B tyrosine phosphorylation and delayed hyperalgesia. The increase in NR2B tyrosine phosphorylation was abolished by intrathecal pretreatment with genistein, a tyrosine kinase inhibitor; PP2, an Src family tyrosine kinase inhibitor; AIDA, a group I metabotropic glutamate receptor antagonist; L733,060, an NK1 tachykinin receptor antagonist, and chelerythrine, a protein kinase C inhibitor. In addition, intrathecal PP2 delayed the onset of mechanical hyperalgesia and allodynia. These findings correlatein vivoNMDA receptor tyrosine phosphorylation with the development and maintenance of inflammatory hyperalgesia and suggest that signal transduction upstream to NR2B tyrosine phosphorylation involves G-protein-coupled receptors and PKC and Src family protein tyrosine kinases.
A Path to Masada
Authors: Gratch Ariel
Published: April 2018

This essay, based on six months of participant observation fieldwork at Masada National Park, offers an experimental tour of the site. The main text is an amalgamation of Youth Israel Experience Tours while the footnotes serve as a dialectical response, commenting on and critiquing the tour structure and post-tourist practice. This essay suggests that the meaning and significance of place, particularly while on tour, is found in the relationship between tourists more so than in the mythical stories told about the site.
The Irish Hills of Michigan
Authors: Gratch Lyndsay Michalik
Published: April 2018

Grounded in the Irish Hills of Michigan, this essay offers performative curation as a method to address the complexities of virtual tourist experiences, in terms of postindustrial, rural, roadside tourism and nostalgic memory. Performative curation juxtaposes tourist accounts, “official” histories, autoethnography, tall tales, dramatic scripts, descriptions of physical artifacts, images, and other texts. As a hybrid of performative writing, collage, and digital content curation, the method is reflexive about the mediation involved in visiting, representing, and remembering tourist locations, and asks the reader to take an explicitly active and ethical role in the virtual tour and meaning-making process.
The acute effect of exposure to noise on cardiovascular parameters in young adults
Authors: Lu Shih-Yi , Lee Cheng-Lung , Lin Kuei-Yi , Lin Yen-Hui
Published: April 2018

Objective

In this study, an experiment was conducted to examine whether noise exposure produced acute changes in cardiovascular responses, and whether these responses differed based on psychoacoustic parameters to noises of low to high intensity.

Methods

Thirty healthy subjects were enrolled. Three industrial noises were binaurally presented with a supraaural earphone. The sound levels of noise were <55, 75, and 90 dB. Each noise was continued for 20 min and the electrocardiogram was simultaneously recorded.

Results

The results showed a statistically significant increase in systolic blood pressure (SBP) at the 90 dB sound level. The study estimated a blood pressure increase of 0.85 mmHg/10 dB and 0.71 mmHg/10 dB in SBP and diastolic blood pressure (DBP), respectively. These results suggest that exposure to noise, particularly high-frequency noise, negatively impacts blood pressure. The tonality and fluctuation strength of noise especially impacts systolic blood pressure.

Conclusions

Th psycho-acoustic parameters of noise should be considered when evaluating the impact of noise exposure.
PENILAIAN KESIAPAN MALUKU SEBAGAI LUMBUNG IKAN NASIONAL
Authors: Suryawati Siti Hajar , Tajerin Tajerin
Published: April 2018

Maluku merupakan propinsi kepulauan dengan potensi sumberdaya perikanan tangkap yang besar. Potensi tersebut meliputi kelompok jenis ikan pelagis besar seperti tuna dan cakalang, pelagis kecil, demersal, udang, cumi-cumi dan ikan karang. Hal tersebut mendorong pemerintah menjadikan wilayah Maluku menjadi lumbung ikan nasional (M-LIN). Penelitian ini bertujuan untuk menganalisis status kesiapan Maluku sebagai lumbung ikan nasional. Metode analisis yang digunakan adalah Multi Dimensional Scaling (MDS) dala bentuk RAP-MLIN (Rapid Appraisal for Maluku as ‘Lumbung Ikan Nasional’) yang merupakan modifikasi dari software RAPFISH (Rapid Appraisal for Fisheries). Hasil analisisnya dinyatakan dalam bentuk indeks dan kesiapan program tersebut. Analisis leverage dan Monte-Carlo digunakan untuk mengetahui faktor pengungkit yang merupakan atribut-atribut yang sensitif terhadap indeks dan status kesiapan. Hasil penelitian menunjukkan bahwa pada dimensi ekologi statusnya cukup siap (50,33%), dimensi ekonomi cukup siap (67,62%), dimensi sosial siap (92,37%), dimensi teknologi siap (99,90%), dimensi infrastruktur cukup siap (70,56%), dan dimensi kelembagaan dan kebijakan siap (86,26%). Dari 47 atribut yang dianalisis, terdapat 18 atribut yang merupakan faktor pengungkit terhadap indeks dan status kesiapan, sehingga perlu dilakukan upaya perbaikan atau intervensi terhadap atribut-atribut tersebut. Dengan melakukan intervensi terhadap 18 faktor tersebut diharapkan dapat meningkatkan status kesiapan Maluku sebagai Lumbung Ikan Nasional ke tingkat yang lebih siap.
STATUS KEBERLANJUTAN SUMBER DAYA PERIKANAN DI PERAIRAN BENGKULU
Authors: Erwina Yuyun , Kurnia Rahmat , Yonvitner Yonvitner
Published: April 2018

Pengelolaan sumber daya perikanan belum berhasil menuaikan kesejahteraan. Untuk itu perlu dilakukan penelitian dengan tujuan menganalisis keberlanjutan sumber daya perikanan di perairan Bengkulu. Penelitian dilakukan pada awal Oktober sampai dengan akhir November 2014. Lokasi penelitian di Provinsi Bengkulu. Penelitian ini menggunakan data primer dan data sekunder. Data primer diperoleh dari pengamatan langsung dan wawancara (200 responden) dengan metode purposive sampling dan pengukuran panjang ikan dominan yang tertangkap yaitu : Ikan kape-kape (Psenes sp) (1.217 ekor), ikan bleberan (Thryssa sp) (699 ekor) dan tenggiri (Scomberomorus sp) (492 ekor). Analisis data dilakukan dengan menggunakan metode Multi Dimensional Scaling (MDS)melalui pendekatan RAPFISH (Rapid Asessment Technique for Fisheries). Penentuan prioritas strategi kebijakan menggunakan analisis kobe plot. Hasil kajian menunjukkan bahwa status keberlanjutan pengelolaan sumber daya perikanan termasuk dalam kategori kurang berkelanjutan dengan nilai indeks 47,109, nilai stress 12,8% dan nilai R2 sebesar 95,3%. Strategi pengelolaan yang harus dilakukan untuk menjaga dan meningkatkan keberlanjutan sumber daya perikanan adalah: Strategi restorasi (0–5 tahun), strategi pengembangan sosial (5–10 tahun) dan strategi keberlanjutan (10–15 tahun). Atribut yang menjadi prioritas untuk diperbaiki dalam rangka meningkatkan status keberlanjutan pengelolaan sumber daya perikanan di perairan Bengkulu adalah harga jual ikan, pemanfaatan pengetahuan lokal yang terkait dengan pengelolaan perikanan, atribut tingkat konflik antar nelayan dan atribut peranan lembaga keuangan mikro/ kelompok usaha bersama.
pilot randomised clinical trial comparing the effect of video game dance training with ladder drills on agility of elite volleyball players
Authors: Roopchand-Martin Sharmella , Chong Ricardo A. , Facey Alison , Singh Praimanand , Mansing Akshai
Published: April 2018

This randomised pilot study compared the effects of a six-week, dance video game training programme with traditional agility ladder drills. Twenty-seven elite volleyball players participated and the Illinois Agility Test was used as the primary outcome measure. Significant improvement was seen in agility scores of the video game dance group with both an intention-to-treat analysis (ITTA), (median [Mdn] = –0.95, p = 0.028), and per-protocol analysis (Mdn = –1.58, p = 0.012). The ladder drills group showed no significant change in agility with the ITTA (Mdn = –0.71, p = 0.062), but improvement was seen with the per-protocol analysis (Mdn = –0.85, p = 0.028). Between group comparisons showed no significant difference in agility scores for the ITTA (p = 0.650). However, with the per-protocol analysis, the video game dance training group demonstrated a significantly greater improvement in agility scores (Mdn = –1.58 sec; p = 0.029.) compared with the ladder drills group (Mdn = –0.85 sec). Changes observed for both analyses exceeded the minimal detectable change for the Illinois Agility Test, indicating that dance video game training may be a useful tool for clinicians wanting to enhance agility. Further research is warranted in this area.
STUDY OF THE EFFECT OF SOIL CEMENT ELEMENTS WHEN STABILIZING ROADBED MODEL IN LABORATORY CONDITIONS
Authors: Petrenko V. D , Tiutkin O. L. , Sviatko I. O.
Published: April 2018

Purpose.Experimental studies allow determining th estress-strain state or bearing capacity of the tested soil body. A preliminary study of the results of model testing and experimental research allows us to find the optimal solutions and to justify the parameters of the chosen technology. The purpose of this work is to determine the effect of soil cement elements when stabilizing the roadbed on a weak subgrade using the soil tests in laboratory conditions. Methodology. During the development of measures for the reconstruction and consolidation of soil bodies, their strength is tested using many methods. In all cases, we take into account the physical and mechanical characteristics of soils obtained as a result of research, as well as the existing regulatory documents. We performed the experimental studies by model testing in laboratory conditions. The model testing was based on the corresponding relationships between geometric sizes, mechanical properties of materials, loads and other factors on which the stress-strain state depends. During testing, the model was loaded gradually. We maintained each load level up to conditional stabilization of the soil deformation. We took the readings from measuring devices at each stage of loading after achievement of stabilization of soil mass deformations. We fixed the readings in the test journal. Findings. During stabilization with soil cement piles there is an improvement in mechanical properties, which leads to a decrease in deformations by 2…3.5 times. Each test is accompanied with graphs of relative deformations-stress dependence, as well as deformation curves and compression curves. According to the results of experimental stu-dies, it can be seen that when testing a model with cement piles, compared with a model without soil cement piles, the relative deformations of the samples taken prior to the experiment and after the experiment almost coincide, indicating a decrease in deformability under load. Originality. We proved that this stabilization type positively affects the stress-strain state of the soil body, by increasing its physical and mechanical characteristics. The obtained results allow for rational design of subgrade stabilization work with the help of modern technologies.Practicalvalue. The in-depth analysis of the results of experimental studies opens up possibilities for theoretical synthesis and development of theoretical foundations, which will allow in the future avoiding, in the similar cases, the experimental verification of the stabilization type given for the relative type of soil. It is confirmed that this method of stabilization used can be applied in real conditions.
KORUMA SORUMLULUĞU: DARFUR KRİZİ VE LİBYA MÜDAHALESİ ÇERÇEVESİNDE BİR DEĞERLENDİRME
Authors: SÜRÜCÜOĞLU Orkun
Published: April 2018

1990’lı yıllarda
düzenlenen uluslararası askeri müdahaleler, insan haklarının korunması
düşüncesi ile devletin egemenliği ilkesi arasında gerilim oluşturmuş ve bu
müdahalelerin hukukiliği tartışmaya açılmıştır. Bu konuda normatif bir
konsensüs oluşturmak üzere kurulan komisyonun (International Commission on
Intervention and State Sovereignty) “koruma sorumluluğu” kavramıyla ortaya
koyduğu görüşler kimi değişikliklerle 2005 Birleşmiş Milletler (BM) Dünya
Zirvesi’nin Sonuç Bildirgesi’nde kendine yer bulmuştur. Özellikle 2011 yılında
Libya’ya yapılan askeri müdahaleden sonra uluslararası kamuoyunda koruma
sorumluluğu kavramı tekrar gündeme gelmiştir. Bu çalışmanın amacı, koruma sorumluluğunun
hukuki bir norm statüsüne ulaşıp ulaşmadığını tartışmaktır. Bunun için
Darfur’da neden askeri müdahale yapılamayıp da Libya’da yapıldığı irdelenmekte
ve bu karşılaştırma üzerinden çıkarımlarda bulunulmaktadır. Çalışma, koruma
sorumluluğunun henüz hukuki bir norm olmadığı, ancak ahlaki bir norm olarak öne
çıktığını ileri sürmektedir. İlgili durumlarda askeri müdahalenin yapılmasında
BM Güvenlik Konseyi’nin daimi üyelerinin çıkarları ve bölgesel örgütlerin tavrı
temel etken olmaktadır. Müdahale seçeneğinin olay bazlı değerlendirme sonucunda
gündeme gelmesi ve devletleri gerektiğinde müdahaleye zorlayacak bir mekanizma
bulunmaması da bu bağlamda önemli eksiklikler olarak göze çarpmaktadır.
11-13 YAŞ ERKEK ÇOCUKLARDA FUTBOL ANTRENMANLARININ LİPİD PROFİLİ VE KAS HASARI BELİRTEÇLERİNDE İYİLEŞTİRİCİ ETKİSİ
Authors: Selçuk Muzaffer , Aslan Tahir Volkan , Temur Hacı Bayram , Çınar Vedat
Published: April 2018

Bu çalışmanın amacı 11-13 yaş erkek çocuklarda futbol
antrenmanlarının lipit profili ve kas hasarı belirteçlerinde iyileştirici etkisini
araştırmaktır. Çalışmanın örneklem grubunu Van Dumlupınar İlköğretim Okulu'nda öğrenim
gören 11-13 yaşlarındaki toplam 40 gönüllü öğrenci oluşturmaktadır. Katılımcılara
8 hafta boyunca, haftada 3 gün olmak üzere düzenli olarak ortalama 2 saat
çeşitli şiddetlerde futbol çalışmaları yaptırıldı. Veriler uygulanan egzersiz
öncesi dinlenik durumda ve egzersiz sonrası dinlenik durumunda iken elde
edilmiştir. Egzersiz öncesi HDL düzeyi 45,91±12,32 iken egzersiz sonrası HDL
düzeyi 52,05±14,04 olarak belirlenmiş olup istatiksel olarak anlamlı bir sonuç
görülmüştür (p< 0.01). CK düzeyi çalışma öncesi dinlenik durumda iken 359,50±138,06
iken çalışma sonrası 247,15±119,54 olarak tespit edilmiş olup anlamlı bir sonuç
görülmüştür (p< 0.01). Yine LDL, Kolesterol ve LDH düzeylerinde değişmeler
görülmüş olup istatistiksel olarak anlamlı bir sonuç tespit edilmiştir (p<
0.01). Kontrol grubu HDL, LDL, Kolesterol, CK, LDH parametlerinde ise herhangi
bir anlamlı sonuç tespit edilmemiştir (p>0.05). Düzenli olarak yapılan
futbol çalışmalarının çocukların biyokimyasal parametrelerinde olumlu
değişimlere neden olabileceği, sağlığı olumlu yönde etkileyebileceği
düşünülmektedir.
GENÇ YETİŞKİN KADINLARDA DÜZENLİ AEROBİK EGZERSİZLERİN İNFLAMATUAR BELİRTEÇLER ÜZERİNE ETKİSİ
Authors: Koca Halit Buğra , Yıldırım İrfan , Işık Özkan , Koca Tülay , Bal Tuncay
Published: April 2018

Bu çalışmanın amacı sekiz haftalık düzenli aerobik egzersizlerin genç yetişkin kadınlarda inflamatuar belirteçler üzerine etkisini incelemektir. Araştırmaya sedanter 27 genç yetişkin kadın gönüllü olarak katıldı. Katılımcılar sekiz hafta, haftada 3 gün, günde 75 dk düzenli aerobik egzersizler gerçekleştirdiler. Sekiz haftalık aerobik egzersizler öncesi ve sonrası katılımcılardan alınan kan örneklerinde C-reaktif protein (CRP), interlökin-6 (IL-6) ve tümor nekrozis faktör-alfa (TNF-α) düzeyleri belirlendi. Araştırmada öntest-sontest ölçümleri arası farklılıklar Wilcoxon Signed Rank testi ile belirlendi. Araştırma bulgularına göre, katılımcıların TNF-α, IL-6 ve CRP düzeylerinin ön test ve son test değerleri arasında istatistiksel olarak anlamlı bir fark olduğu tespit edilmiştir. Bu bulgular 8 haftalık düzenli aerobik egzersizlerin, katılımcıların serum TNF-α, IL-6 ve CRP düzeylerini anlamlı olarak düşürdüğü ve düzenli aerobik egzersizlerin kronik hastalıklara yakalanma riskini azaltabileceğini düşündürmektedir.
“That German stuff”: Negotiating Linguistic Legitimacy in a Foreign Language Classroom
Authors: Ennser-Kananen Johanna
Published: April 2018

This qualitative case study of one German suburban high school classroom in the Midwestern United States examines how learners of German negotiate their linguistic legitimacy, which is defined as discursively constructed acceptance or validation for their language use. Specifically, it investigates how the students negotiated legitimacy for using their target language German in their classroom. Based on the premise that linguistic legitimacy is crucial for the maintenance and development of speakers’ languages, data was collected and analyzed from classroom recordings, semi-structured interviews, and participant observations. Findings revealed that, while English dominated the lessons as the default legitimate language among the students, using German was accepted and valued under certain circumstances. Such instances of linguistic legitimacy included the use of German for entertainment or in role plays, a pattern which points to the students’ desire to mitigate investment and display “uninvestment” in learning or using German. Implications for foreign language (FL) pedagogy and teacher education are discussed.
Colony PCR with ready-to-load PCR master mix (similar to GoTaq) v2
Authors: Floor Stephen
Published: April 2018

This protocol makes a master mix for PCR that is ready to load into an agarose gel. Useful for colony PCR and related.
Dengue virus type 4 (DENV-4) TaqMan (DENV4-TM2017) assay v1
Authors: M Mackay Ian , A Northill Judy
Published: April 2018

This protocol was designed and developed at this laboratory. The assay specifically targets the 3' UTR region of DENV-4 strains and is designed as a qualitative screening test for human cases of DENV-4 infection, but not for infection due to other known DENVs.
INVERSE PROBLEMS IN THE HEAT AND MASS TRANSFER THEORY
Authors: Pyatkov Sergey Grigorievich
Published: April 2018

This article is a survey of the recent results obtained preferably by the author and its coauthors and devoted to the study of inverse problem for some mathematical models, in particular those describing heat and mass transfer and convection-diffusion processes. They are defined by second and higher order parabolic equations and systems. We examine the following two types of overdetermination conditions: a solution is specified on some collection of spatial manifolds (or at separate points) or some collection of integrals of a solution with weight is prescribed. We study an inverse problem of recovering a right-hand side (the source function) or the coefficients of equations characterizing the medium. The unknowns (coefficients and the right-hand side) depend on time and a part of the space variables. We expose existence and uniqueness theorems, stability estimates for solutions. The main results in the linear case, i.e., we recover the source function, are global in time while they are local in time in the general case. The main function spaces used are the Sobolev spaces.
PARTICLE DYNAMICS WITH A VISCOUS LIQUID THROUGH A POROUS MEDIUM CELL WITH A PRESSURE GRADIENT
Authors: Martynov Sergei Ivanovich , Deryabina Maria Sergeevna
Published: April 2018

On the basis of the previously developed mathematical model, the dynamics of the flow of a viscous liquid with particles in a porous medium is studied. The model takes into account the hydrodynamic interaction of all particles, both moving and stationary. For computer simulation of particle dynamics in such currents, a developed software package is used. A numerical calculation of the dynamics of a viscous liquid with particles at a given pressure gradient in two model structures of a porous medium, formed respectively of 450 and 478 particles of effective size is carried out. The dimensions of the dispersed particle placed in a viscous liquid were 0.3 and 0.2 of the effective particle size. The flow of a viscous liquid with a pressure gradient was specified. It is found that in the presence of a pressure gradient, the movement of the dispersed particle in the porous structure has a component directed against the flow of liquid outside the structure.
Социальная адаптация трудовых мигрантов из стран Центральной Азии в России
Authors: Зимова Наталья Сергеевна
Published: April 2018

Данная статья посвящена анализу проблемы социальной адаптации трудовых мигрантов из стран Центральной Азии в современном российском обществе. В статье анализируются основные эмпирически фиксируемые показатели социальной адаптации трудовых мигрантов в разрезе таких видов адаптации, как языковая, социальная, экономическая, культурная и профессиональная. Раскрываются проблемы социальной адаптации трудовых мигрантов и причины их появления.
Укрепление связей с диаспорой и привлечение бывших соотечественников как ключевые инструменты государственного регулирования международной трудовой миграции
Authors: Вартанян Алла Аревшадовна
Published: April 2018

В статье приведен анализ примеров успешного взаимодействия стран происхождения трудовых эмигрантов с диаспорами в других странах, выявлена важнейшая роль денежных переводов в развитии экономики стран – доноров рабочей силы, обоснована необходимость проведения программ по репатриации бывших соотечественников.
Granulomatous Lymphangitis Masquerading as Relapsed Hodgkin Disease on FDG PET/CT
Authors: Mustafa Mansour , Patel Dhruv , Shen Lily , Shetty Anita , Mansberg Robert
Published: April 2018

A 38-year-old woman with Hodgkin lymphoma was referred for staging fludeoxyglucose (18F) positron emission tomography/computed tomography (FDG PET/CT) that showed widespread intensely FDG-avid disease in multiple nodal stations above the diaphragm and spleen and extranodal involvement in the lungs and vertebral bodies. She underwent chemotherapy and radiotherapy. Progress FDG PET/CT 5 months later showed significant metabolic and anatomic response. Repeat FDG PET/CT 1 month later was highly suspicious of recurrent disseminated FDG-avid lymphoma in multiple nodal stations above and below the diaphragm, spleen, multiple bones, and lungs. Subsequent bone marrow biopsy showed sarcoid-like granulomatous inflammation with no evidence of lymphoma. The patient was clinically well and no active treatment was instituted. Subsequent FDG PET/CT 2 months later showed complete resolution of metabolic activity.
Hydrothermal Synthesis, Crystal Structures and Properties of 1D Coordination Polymers Based on 5-Nitroisophthalic Acid and 1-Methylimidazole Linkers
Authors: ARICI Mürsel
Published: April 2018

Two new coordination polymers, namely [Mn(µ3-5-nip)(1-meim)2(H2O)]n
(1) and {[Co(µ-5-nip)(1-meim)3]∙H2O}n
(2) (5-nip: 5-nitroisophthalate,
1-meim: 1-methylimidazole), were hydrothermally synthesized and characterized by
IR spectroscopy, elemental analysis, single crystal diffraction and
simultaneous thermal analysis techniques. The 5-nip ligand exhibited two
different coordination modes in its structures. In 1 and 2, 1D chains were
generated by 5-nip ligands and metal (II) ions. In 1 and 2, 5-nip ligand
coordinated to three Mn(II) and two Co(II) ions as bis(monodentate) bridging
mode and a monodentate and bidentate chelating modes, respectively. For 1 and 2, 2D supramolecular layers were formed by hydrogen bonds which
were extend into 3D supramolecular structures via π···π interactions between
two symmetry–related imidazole rings of neighboring molecules. Furthermore,
optical and thermal properties of the complexes were also studied.
A challenging palliation via novel therapeutic approach for unusual presenting with gingival metastasis from renal cell carcinoma relapsed eight years after nephrectomy
Authors: Aydın Asım Armağan , Alparslan Ahmet Şükrü , Ayık Hülya , Kayıkçıoğlu Erkan , Öztürk Banu
Published: April 2018

Renal cell carcinoma (RCC) is the third most
frequently seen primary source of metastatic lesions of the oral cavity. Still
oral cavity metastasis is a rarely seen clinical manifestation during the
course of RCC. In a patient with a known history of RCC, in the presence of
newly emerged oral and maxillofacial lesion(s), possibility of metastasis
should be always thought of and pathological investigation should be performed
as soon as possible. In RCC, metastatic involvement of oral cavity is generally
accompanied by involvement of other organs with resultant lower chance of cure
and poor prognosis. Especially in conditions which adversely affect functions
of chewing and swallowing consequently nutrition and quality of life, surgery
is contraindicated and local interventional methods transarterial
chemoembolization, microwave ablation and radiofrequency ablation can be good
palliative treatment alternatives. In advanced stages of the diseases with
systemic involvement application of local interventional methods either solely
or in combination with radiotherapy and surgery increases compliance to the
systemic treatment and can contribute markedly to the prolongation of survival.
Herein we aimed to present a case with unusual histopathological
characteristics and presentation in whom we achieved a longer period of
disease-free survival using a different local treatment method.
An unusual intraosseous calcaneal lipoma: case report
Authors: Alkan Ender , Erdoğan Hasan , Kara Turgay
Published: April 2018

Intraosseous lipoma, a rare primary benign tumor of
the skeleton, is most commonly found in the calcaneus. It may contain
homogenous fat, but it may also contain necrosis, calcification, or
ossification. It usually does not show contrast enhancement, but there is an
interface enhancement between the outer fat plan and the inner fluid part.
Herein, we present an unusual 22-year-old male case of calcaneal intraosseous
lipoma with contrast enhancement in the fatty component. The lesion was totally
curetted and replaced with bone graft. The pathological diagnosis was reported
as an intraosseous lipoma. The correct diagnosis of an atypical calcaneal
intraosseous lipoma is very important to prevent an unnecessary biopsy and
treatment.
The incidence of polyneuropthy and its relation with disease parameters in chronic obstructive pulmonary disease
Authors: OGAN Nalan , SAYIN Refah , BAHA Ayşe , AKPINAR Evrim Eylem , GÜLHAN Meral
Published: April 2018

Objective: Chronic
Obstructive Pulmonary Disease (COPD) is accompanied by several concomitant
conditions due to its systemic effects. Hypoxia and oxidative stress associated
with COPD are also believed to contribute to the disease course through the
effects of several inflammatory mediators. Major causative agents causing of peripheral
neuropathy are age, hypoxia, duration and severity of COPD, hypercapnia and
smoking. The present study aimed to investigate the incidence of polyneuropathy
and its relation with disease parameters in COPD patients. Methods: Of a total
number of 45 patients who had been followed up between January 1, 2015 and
December 31, 2016 with a diagnosis of COPD at Ufuk University School of
Medicine were enrolled retrospectively. All
patients underwent electroneuromyography, arterial blood gas measurements and
pulmonary function tests. Patients were divided as those with or without
neuropathy, and factors contributing to the development of neuropathy were
investigated based on clinical and laboratory findings. Results: Eleven (24.4%) patients
were women and 34 (74.6%) were men, and the mean age was
73.2 years. Mean PaO2 was 58.2 mmHg, pCO2 was 41.2 mmHg,
and FEV1 was 45.3%. Neuropathy was diagnosed in twenty (44.4%)
patients. Significant
differences were found in pO2, pCO2, SO2,
mMRC, smoking status and number of exacerbations per year (p < 0.05). Conclusions: Patients who experience
frequent exacerbations and those with hypoxemia or hypercapnia as demonstrated
should be taken into more careful clinical assessments with respect to polyneuropathy.
Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls
Authors: Divya P. , , , Ramesha K. P. , Kumari Ragini , Singh Arun Pratap , Das D. N. , Basavaraju M. , Mundhe U. T.
Published: April 2018

Selection of high fertile bulls with the help of marker assisted selection has gained importance in recent years. The low heritability of fertility traits hampers improvement of these traits by conventional selection based on phenotypic records. No information is available on the role of SNPs in KiSS1 gene in cattle on semen quality parameters in bovines. KiSS1 genes code for Kisspeptin, which are essential upstream regulators of neurons secreting gonadotropin-releasing hormone and play crucial role in reproduction.The coding regions along with exon-intron boundaries of KiSS1 gene, was characterized using PCR-SSCP method and direct sequencing. Two genotypes were observed which were represented as SSCP pattern 1 and pattern 2 and found to carry one SNPs (T153C) and one insertion of G at 291_292bp. The bulls with pattern 2 were heterozygous with respect to the transition T153C and pattern1 bulls were homozygous with TT genotype. The transition was predicted to cause amino acid change from Valine to Alanine. The frequency of bulls with pattern1 and pattern 2 were 0.67 and 0.33 in 67 Holstein Friesian bulls and 0.73 and 0.27 in 13 Khillari bulls. The association study of genotypes with semen quality parameters revealed significant association of genotypes with acrosome integrity in fresh semen (P less than 0.05) and no association with sperm concentration, volume per ejaculate, percent live sperm and Hypo Osmotic Swelling Test (HOST) with higher acrosome integrity in bulls with pattern2. Upon validation of the results in larger population and identifying the exact role of the novel SNP T153C and insertion of G at 291_292bp, they could be incorporated in selection programme for improving fertility in bulls.as markers for acrosome integrity in cattle.
Effect of supplementation of L. plantarum and L.gv based probiotic milk powder on growth performances and nutrient utilization of Charles Foster rats
Authors: Singh Prity , Pandey Rajendra Kumar , Paswan Vinod Kumar , Yadav Satya Prakash , Bhinchhar Basant Kumar , Singh Chandra Shekhar
Published: April 2018

Effects of the supplementation of L. plantarum and L. casei based spray dried probiotic milk powder was investigated for growth performances, nutrient utilization and blood biochemistry including the cholesterol lowering effect in Charles Foster (CF) rats. Forty-eight male CF rats (21 day old) were divided into four groups and fed on basal diets supplemented with plane spray dried milk powder without any probiotic (control) and L. plantarum, L. casei and the mixed culture of L. plantarum and L. casei based spray dried probiotic milk powder, respectively in LP, LC and LPLC groups. Supplementation of diets with probiotic milk powder containing either the monoculture of L. plantarum or L. casei or their mixed culture improved growth, feed conversion ratio and nutrient utilization. Improved health performance was reflected in hemato-clinical chemistry including the hypocholesterolemic effect of the probiotic milk powder supplemented experimental rats. Although the improvement in growth, nutrient utilization and health of experimental rats was observed in all the three probiotic milk powder supplemented groups viz. LP, LC and LPLC; however these improvement was more in the L. plantarum or L. casei mixed culture supplemented group as compared to other two monoculture probiotic milk powder supplemented groups.
Biometric analysis of testicles of the Kafue lechwe (Kobus leche kafuensis) and Bangweulu lechwe (Kobus leche smithemani) in Zambia
Authors: Zulu Victor Chisha , Mwanza Alfred Malenga , Yoshida Mitsutoshi , Syakalima Michelo , Yasuda Jun
Published: April 2018

The Kafue lechwe (Kobus leche kafuensis) is a unique antelope found only in the Kafue basin of Zambia and is the main source of local income and protein. Its population has been dwindling over the years supposedly due to poaching and environmental pollution in the area and without mention of reproductive causes. The present study was a pilot study which determined biometric measurements of the testicles in adult lechwes as measures of reproductive ability and fertility. Testicles from 24 Kafue and 24 Bangweulu lechwes were examined from August to October 2009. The measurements were taken using Calipers, a ruler and string for testicular length, width, height, head size, body and tail of the epididymis. All testicular parameters were significantly higher in the Kafue than the Bangweulu lechwe. The study also found cases of cryptorchidism and abnormal epididymis in the Kafue and Bangweulu lechwes, respectively. The testicular parameters were measured during the non-breeding season thus influence of breeding on testicular biometry is expected. This was the first study of its kind and further studies on the reproductive physiology and biology are required to understand various causes of reductions in population and thereby aid the conservation of this species.
Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek
Authors: Lekhi Prince , Gill R. K. , Kaur Satinder , Bains T. S.
Published: April 2018

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.
Young Adult Victimization and Midlife Consequences: Sensitization or Steeling Effects of Childhood Adversity?
Authors: Doherty Elaine Eggleston , Jaecques Brittany , Green Kerry M. , Ensminger Margaret E.
Published: April 2018

The interrelationship between victimization, violence, and substance use/abuse has been well established, yet those who experience victimization do not necessarily respond with violence or substance use or escalate to experiencing substance abuse symptoms. Drawing on literature from both the syndemic research from medical anthropology and the resilience research from psychology, this study examines the interaction between early childhood adversity and young adult violent victimization on later substance use/abuse and violent offending to provide insight into conditional effects. Data are derived from the Woodlawn Study, an African American cohort of men and women from a socioeconomically heterogeneous community in the South Side of Chicago, who were followed from first grade through age 42. Results indicate that those with lower levels of childhood adversity are more likely to suffer the negative consequences of violent victimization than those with higher childhood adversity, providing support for a “steeling” effect.
Penentuan Secara Imunopatologi Organ Target Virus Flu Burung Menggunakan Streptavidin Biotin (DETERMINATION OF TARGET ORGANS OF AVIAN INFLUENZA VIRUS USING IMMUNOPATHOLOGICAL IMMUNOHISTOCHEMISTRY STREPTAVIDIN-BIOTIN)
Authors: Yunita Niken , Wulan Ocie Harum , Wuryastuty Hastari , Wasito Raden
Published: April 2018

Avian influenza is a viral disease in poultry caused by avian influenza virus (AIV) subtype H5N1 with varying clinical signs are often similar to the clinical signs of other viral infections, such as Newcastle disease virus (NDV). The mechanism of disease pathogenesis to express clinical signs tightly correlated to the determination of the target organ seen from AIV H5N1 antigens distribution in organs, such as respiratory tract, brain and gastrointestinal tract. Immunopathological immunohistochemistry streptavidin-biotin (IHC SB) is a method for sensitive and accurate in detecting antigens of AIV on the tissues. In the present study, it was determined whether in laying hens with clinical signs of torticollis and curled toe paralysis, and pathologic anatomic lesions in the form of petechial and foci necrotic hemorrhages tested with immunopathological IHC SB is positive AIV H5N1 infection. IHC SB study results showed that the AIV H5N1 antigen were found in tissues of the lung, brain, duodenum and proventriculus. Based on these results, we can conclude that the IHC SB is a method that is highly sensitive and accurate to detect H5N1 antigens and its distribution in the host.
Profil Protein Trypanosoma evansi dari Daerah Geografis Berbeda di Indonesia Tahun 2012-2014 dengan Sodium Dodecil Sulphate Polyacrylamide Gel Electrophoresis (TRYPANOSOMA EVANSI PROTEIN PROFILE OF DIFFERENT GEOGRAPHICAL AREAS ORIGIN IN INDONESIA
Authors: Ekawasti Fitrine , Yuniarto Ichwan , Sulinawati Sulinawati , Subekti Didik Tulus
Published: April 2018

Surra outbreak in 2012 has led to more than 1,700 animals have died in the province of East Nusa Tenggara (NTT) Indonesia. Surra case sporadically continues throughout the year in various areas, especially Kalimantan, Banten as well as other areas. Some reports reveal differences in protein profiles among multiple isolates of T. evansi. Therefore the purpose of this research were to find out the protein profile of each isolate T. evansi in Indonesia and the possible biological differences among them. Eleven isolates originating from the province of East Nusa Tenggara, South Kalimantan and Central Kalimantan, Banten, Lampung and Bengkulu has been isolated and purified Using DEAE. Trypanosoma isolate were frezeethawing repeatedly to obtain soluble protein. Furthermore, soluble protein is treated with heating or without heating and then each was run on SDS PAGE with Coomassie Blue staining. The protein profiles of all isolates were compared each other. The results showed that eleven isolates of T. evansi in Indonesia has a very diverse protein profile. Then for the purposes of development of diagnostic kit can be used whole lysate cell (WCL) as stock antigen in serological test process.
Genetic Diversity and Molecular Phylogeny of Iranian Goats Based on Cytochrome Oxidase I (COXI) Gene Sequences (KERAGAMAN GENETIK DAN FILOGENI MOLEKULER KAMBING-KAMBING IRAN BERDASARKAN SEKUENS GEN CYTOCHROME OXIDASE I (COXI))
Authors: Sharifi Reza Seyed , Sofla Sima Savar , Seyedabadi Hamid Reza
Published: April 2018

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.
Dog Demography And Level Of Knowledge Against Rabies In Positive And Negative Case Of Rabies Areas In Mendoyo-Jembrana, Bali, Indonesia (DEMOGRAFI ANJING DAN TINGKAT PENGETAHUAN MASYARAKAT TERHADAP RABIES DI DAERAH DENGAN KASUS RABIES POSITIF DAN NEGATIF
Authors: Agustina Kadek Karang , Cahyani Putu Sasmitha Devi , Suardana Ida Bagus Kade
Published: April 2018

This research aims were to compare the demography of dogs and knowledge profile of dog owners against rabies in Mendoyo Sub-District, Jembarana Bali. This research used observational method, conducted from May to June 2016 in Mendoyo sub-district of Jembrana. Two Banjars were selected by the rabies case report in dogs (YehEmbangKangin village) and two other without case (MendoyoDanginTukad village). The data collected by interviewing the community and direct assessment of free-roaming dogs in the areas. A total of 200 household samples were taken, 50 respondents of each sampling area. This research resulted that the ratio of man-dog in Mendoyo was 4.5:1 and the ratio of male and female dogs was 1.8:1. As many as 64.8% of dog’s age were more than 12 month old, 28% were 4 to 12 month old and 8% were less than 4 month old. In the sampling areas, 19.4% of dogs were sterilized, which were 91.18% males and 8.82% females. Coverage of rabies vaccination in owned dogs was 82.3%. Dog’s petting method by people in Mendoyo was 69.71% free-roaming while 30.29% tied or caged. On free-roaming dog assessment, this study found 52 free-roaming dogs on the streets, 65.38% males and 34.62% females, only 30.8% of those used vaccination collar marker, Based on the body condition score, 76.9% of free-roaming dogs have good conditions. Of the total 200 households surveyed, there was a high level of knowledge about the danger of rabies and the importance of vaccination, but only a few people (<35%) received consulting about rabies, understood how to prevent rabies infection and the regulations about dog petting management. In positive area, we found there are still many people who have the habit of disposing dog. We conclude that there is needed to improve knowledge and attitude of the community about the prevention and risk factor of rabies.
CARTOGRAFIA GEOMORFOLÓGICA REGIONAL E MORFOGÊNESE: CONTRIBUIÇÕES METODOLÓGICAS
Authors: Marques Neto Roberto , Ferraro Bernardo Valente
Published: April 2018

A cultura geomorfológica brasileira padece de uma falta de uniformização na representação cartográfica do relevo, o que tem resultado em diversas propostas metodológicas desarticuladas. A necessidade de se integrarem em um mesmo documento cartográfico elementos morfológicos, morfométricos, morfodinâmicos, morfoestruturais e morfocronológicos dificulta que se firme uma metodologia a ser adotada em todos os projetos cartográficos. Seria salutar um avanço em demanda a uma linguagem cartográfica similar aplicável a diferentes escalas, sobretudo nas escalas regionais, estratégicas para um mapeamento sistemático. Em caráter mais oficial, conheceu expressiva difusão as propostas metodológicas construídas ao longo dos anos de execução do Projeto RADAMBRASIL, bem como aquela desenvolvida no âmbito do Instituto Brasileiro de Geografia e Estatística (IBGE). O presente trabalho atina especificamente à interpretação e representação do relevo em escala regional, e, a partir de uma abordagem integrada das duas propostas metodológicas supracitadas, apresenta uma proposta de mapeamento geomorfológico para a Folha Juiz de Fora, na escala de 1/250.000, como aporte à interpretação da morfogênese regional.