bluuebunny/crossref_metadata_2025 huggingface dataset:
MED19 alters AR occupancy and gene expression in prostate cancer cells, driving MAOA expression and growth under low androgen
Authors: Weber Hannah , Ruoff Rachel , Garabedian Michael J.
Published: January 2021

Androgen deprivation therapy (ADT) is a mainstay of prostate cancer treatment, given the dependence of prostate cells on androgen and the androgen receptor (AR). However, tumors become ADT-resistant, and there is a need to understand the mechanism. One possible mechanism is the upregulation of AR co-regulators, although only a handful have been definitively linked to disease. We previously identified the Mediator subunit MED19 as an AR co-regulator, and reported that MED19 depletion inhibits AR transcriptional activity and growth of androgen-insensitive LNCaP-abl cells. Therefore, we proposed that MED19 upregulation would promote AR activity and drive androgen-independent growth. Here, we show that stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation. To delineate the mechanism, we determined the MED19 and AR transcriptomes and cistromes in control and MED19-overexpressing LNCaP cells. We also examined genome-wide H3K27 acetylation. MED19 overexpression selectively alters AR occupancy, H3K27 acetylation, and gene expression. Under conditions of androgen deprivation, genes regulated by MED19 correspond to genes regulated by ELK1, a transcription factor that binds the AR N-terminus to induce select AR target gene expression and proliferation, and genomic sites occupied by MED19 and AR are enriched for motifs associated with ELK1. Strikingly, MED19 upregulates expression of monoamine oxidase A (MAOA), a factor that promotes prostate cancer growth. MAOA depletion reduces androgen-independent growth. MED19 and AR occupy the MAOA promoter, with MED19 overexpression enhancing AR occupancy and H3K27 acetylation. Furthermore, MED19 overexpression increases ELK1 occupancy at the MAOA promoter, and ELK1 depletion reduces MAOA expression and androgen-independent growth. This suggests that MED19 cooperates with ELK1 to regulate AR occupancy and H3K27 acetylation at MAOA, upregulating its expression and driving androgen independence in prostate cancer cells. This study provides important insight into the mechanisms of prostate cancer cell growth under low androgen, and underscores the importance of the MED19-MAOA axis in this process.
Kinetochore-independent mechanisms of sister chromosome separation
Authors: Vicars Hannah , Karg Travis , Warecki Brandt , Bast Ian , Sullivan William
Published: January 2021

Although kinetochores normally play a key role in sister chromatid separation and segregation, chromosome fragments lacking kinetochores (acentrics) can in some cases separate and segregate successfully. InDrosophilaneuroblasts, acentric chromosomes undergo delayed, but otherwise normal sister separation, revealing the existence of kinetochore- independent mechanisms driving sister chromosome separation. Bulk cohesin removal from the acentric is not delayed, suggesting factors other than cohesin are responsible for the delay in acentric sister separation. In contrast to intact kinetochore-bearing chromosomes, we discovered that acentrics align parallel as well as perpendicular to the mitotic spindle. In addition, sister acentrics undergo unconventional patterns of separation. For example, rather than the simultaneous separation of sisters, acentrics oriented parallel to the spindle often slide past one another toward opposing poles. To identify the mechanisms driving acentric separation, we screened 117 RNAi gene knockdowns for synthetic lethality with acentric chromosome fragments. In addition to well-established DNA repair and checkpoint mutants, this candidate screen identified synthetic lethality with X-chromosome-derived acentric fragments in knockdowns of Greatwall (cell cycle kinase), EB1 (microtubule plus-end tracking protein), and Map205 (microtubule-stabilizing protein). Additional image-based screening revealed that reductions in Topoisomerase II levels disrupted sister acentric separation. Intriguingly, live imaging revealed that knockdowns of EB1, Map205, and Greatwall preferentially disrupted the sliding mode of sister acentric separation. Based on our analysis of EB1 localization and knockdown phenotypes, we propose that in the absence of a kinetochore, microtubule plus-end dynamics provide the force to resolve DNA catenations required for sister separation.
Ablation of DNA-methyltransferase 3A in skeletal muscle does not affect energy metabolism or exercise capacity
Authors: Small Lewin , Ingerslev Lars R. , Manitta Eleonora , Laker Rhianna C. , Hansen Ann N. , Deeney Brendan , Carrié Alain , Couvert Philippe , Barrès Romain
Published: January 2021

In response to physical exercise and diet, skeletal muscle adapts to energetic demands through large transcriptional changes. This remodelling is associated with changes in skeletal muscle DNA methylation which may participate in the metabolic adaptation to extracellular stimuli. Yet, the mechanisms by which muscle-borne DNA methylation machinery responds to diet and exercise and impacts muscle function are unknown. Here, we investigated the function of de novo DNA methylation in fully differentiated skeletal muscle. We generated muscle-specific DNA methyltransferase 3A (DNMT3A) knockout mice (mD3AKO) and investigated the impact of DNMT3A ablation on skeletal muscle DNA methylation, exercise capacity and energy metabolism. Loss of DNMT3A reduced DNA methylation in skeletal muscle over multiple genomic contexts and altered the transcription of genes known to be influenced by DNA methylation, but did not affect exercise capacity and whole-body energy metabolism compared to wild type mice. Loss of DNMT3A did not alter skeletal muscle mitochondrial function or the transcriptional response to exercise however did influence the expression of genes involved in muscle development. These data suggest that DNMT3A does not have a large role in the function of mature skeletal muscle although a role in muscle development and differentiation is likely.
ATG5 is instrumental in the transition from autophagy to apoptosis during the degeneration of tick salivary glands
Authors: Wang Yanan , Zhang Houshuang , Luo Li , Zhou Yongzhi , Cao Jie , Xuan Xuenan , Suzuki Hiroshi , Zhou Jinlin
Published: January 2021

Female tick salivary glands undergo rapid degeneration several days post engorgement. This degeneration may be caused by the increased concentration of ecdysone in the hemolymph during the fast feeding period and both autophagy and apoptosis occur. In this work, we first proved autophagy-related gene (ATG) and caspase gene expression peaks during degeneration of the tick salivary glands. We explored the regulatory role ofRhipicephalus haemaphysaloidesautophagy-related 5 (RhATG5) in the degeneration of tick salivary glands. During the fast feeding phase, RhATG5 was cleaved and both calcium concentration and the transcription of Rhcalpains increased in the salivary glands. Recombinant RhATG5 was cleaved by μ-calpain only in the presence of calcium; the mutant RhATG5191-199Δwas not cleaved. Treatment with 20-hydroxyecdysone (20E) led to programmed cell death in the salivary glands of unfed ticksin vitro, RhATG8-phosphatidylethanolamine (PE) was upregulated in ticks treated with low concentration of 20E. Conversely, RhATG8-PE decreased and Rhcaspase-7 increased in ticks treated with a high concentration of 20E and transformed autophagy to apoptosis. High concentrations of 20E led to the cleavage of RhATG5. Calcium concentration and expression of Rhcalpains were also upregulated in the tick salivary glands. RNA interference (RNAi) of RhATG5in vitroinhibited both autophagy and apoptosis of the tick salivary glands. RNAi of RhATG5in vivosignificantly inhibited the normal feeding process. These results demonstrated that high concentrations of 20E led to the cleavage of RhATG5 by increasing the concentration of calcium and stimulated the transition from autophagy to apoptosis.
Ilomastat contributes to the survival of mouse after irradiation via promoting the recovery of hematopoietic system
Authors: Zhao Baoquan , Li Xiaoman , Li Xingzhou , Quan Dongqin , Zhang Fang , Hu Burong
Published: January 2021

Ilomastat, a broad-spectrum inhibitor of matrix metalloproteinases (MMPs), has drawn attentions for its function in alleviating radiation damage. However, the detailed mechanisms of Ilomastat’s protection from animal model remain not fully clear. In this study, the C57BL/6 mice were pre-administrated with Ilomastat or vihicle for 2 h, and then total body of mice were exposed to 6 Gy of γ-rays. The protective effect of Ilomastat on the hematopoietic system in the irradiated mice were investigated. We found that pretreatment with Ilomastat significantly reduced the level of TGF-β1 and TNF-α, and elevated the number of bone marrow (BM) mononuclear cells in the irradiated mice. Ilomastat pretreatment also increased the fraction of BM hematopoietic progenitor cells (HPCs) and hematopoietic stem cells (HSCs) at day 30 after irradiation, and protected the spleen of mouse from irradiation. These results suggest that Ilomastat promotes the recovery of hematopoietic injury in the irradiated mice, and thus contributes to the survival of mouse after irradiation.
Colostrum avoidance practice and associated factors among mothers of children aged less than six months in Bure District, Amhara Region, North West, Ethiopia: A community-based cross-sectional study
Authors: Mose Ayenew , Dheresa Merga , Mengistie Bezatu , Wassihun Biresaw , Abebe Haimanot
Published: January 2021

Introduction

The World Health Organization and the United Nations International Children’s Emergency Fund jointly recommend colostrum feeding immediately following delivery of the newborn. Colostrum avoidance is the practice of discarding colostrum at least once within the first three days after delivery of the newborn. Colostrum contains antibodies that protect the newborn against disease. Despite this fact, many Ethiopian mothers see colostrum feeding as a cause of neonatal morbidity and mortality, and they believe colostrum should be discarded to alleviate its effects. However, the cause of this misconception about colostrum is not well researched in Ethiopia, particularly in the study area.

Objective

To assess the prevalence of colostrum avoidance and associated factors among mothers of children aged less than six months in Bure district, Amhara Region, North West, Ethiopia.

Methods

A community-based cross-sectional study was conducted from March 1st to 30th, 2019 in Bure district. Structured questionnaires and face to face interviews were used to collect data from 621 study participants. Multistage sampling technique was used to select study participants. Data were entered into Epi Data version 4.2.0 and then exported to Statistical Package for the Social Sciences version 23. Bivariate and multivariate logistic regression analyses were performed to identify predictors of colostrum avoidance practice.

Results

Six hundred twenty-one (621) mothers of children aged less than six months participated. The prevalence of colostrum avoidance was 14.5% (95%CI; 11.5–17.4). The multivariate analysis indicated that home delivery [AOR = 3.350, (95%CI; 2.517–14.847)], giving birth through cesarean section [AOR = 3.368, (95%CI; 1.339–8.471)], no participation in an antenatal care group [AOR = 1.889, (95%CI; 1.144–3.533)], poor knowledge of mothers about colostrum [AOR = 3.44, (95%CI; 2.45–4.977)], and poor attitude of mothers towards colostrum [AOR = 3.053, (95%CI; 1.157–8.054)] were important predictors of colostrum avoidance practice.

Conclusion and recommendations

Home delivery, giving birth through cesarean section, no participation in an antenatal care group, poor knowledge of mothers about colostrum, and poor attitude of mothers towards colostrum were significantly associated with colostrum avoidance practice. Therefore, health care workers in the district should promote institutional delivery, reduce the magnitude of cesarean section, encourage pregnant mothers to participate in an antenatal care group, and enhance maternal awareness about the merits of colostrum feeding. Moreover, health educations for mothers to have a positive attitude towards colostrum are important recommendations to be taken to prevent the further practice of colostrum avoidance.
OPEX: Development of a novel overall patient experience measure to facilitate interpretation of comparison effectiveness studies
Authors: Fraenkel Liana , Wei Zhenglin , Ramsey Christine , Wiedmeyer Carole , Michaud Kaleb , Neogi Tuhina , Nowell W. Benjamin , Venkatachalam Shilpa , Broniatowski David A.
Published: January 2021

Objectives

A measure that encompasses both benefits and harms at the individual patient level may facilitate comparisons between treatment options and improve shared decision-making. The objective of this study was to develop a patient reported measure to capture overall experience (including both benefits and harms) of treatment using rheumatoid arthritis (RA) as a case example.

Methods

Hierarchies for treatment benefits are known. Therefore, we developed a hierarchy of adverse events (AEs) using a series of trajectory mapping and paired comparison surveys. We subsequently used these data to construct a paired comparison survey, asking patients to compare options including both a specified level of benefit and an AE. These data were used to generate a hierarchy of overall experience on treatment.

Results

782 participants completed a series of three surveys. The trajectory mapping procedure and a paired comparison survey led to the generation of a hierarchy of AEs with nine levels ranging from No AEs to irreversible serious complications. In a third survey, in which AEs were paired with benefits, participants’ ratings generated a 6-level hierarchy of overall experiences ranging from Major improvement + No, mild or manageable AEs (Level 1) to No improvement + Irreversible AEs (Level 6).

Conclusions

Using a trajectory mapping approach, we developed a patient reported measure representing the distribution of patients’ overall experiences on treatment. The intent of this measure is to enable patients and their physicians to compare the percentage of patients experiencing each level of outcome, from most to least desirable, across treatments.
Effect of timing of implementation of containment measures on Covid-19 epidemic. The case of the first wave in Italy
Authors: Timelli Laura , Girardi Enrico
Published: January 2021

There is evidence that adoption of non-pharmaceutical containment measures (NPMs) may have had a major impact on Covid-19 epidemic dynamics, and mitigated its effect on healthcare system. Optimal timing of implementation of these measures however is not known. In Italy, a national lockdown was decided on March 11th 2020 and ended 4th of May. At that time, cumulative incidence (CI) was different in Italian regions which ranged from <5 cases/100,000 to >11 cases/100,000 inhabitants. In this paper, we aim to evaluate how level of incidence in different regions at the time of implementation of NPMs affected CI and had an impact on the healthcare system in terms of ICU bed occupancy and mortality rates. We used regional daily new COVID-19 diagnosed cases as well number of people hospitalized in ICU and number of deaths for period February 24-May 11 from all the 19 Italian regions and two autonomous provinces. For each region we calculated: temporal daily trend of cumulative cases of Covid-19/100,000 inhabitants, daily trend of ICU bed occupancy and mortality rate at the end of period. We found that the epidemic curves show similar trends for all regions and all tend to flatten between 11–32 days. However, after 2 months, regions with lower CI at lockdown remained at substantially lower CI (<265 cases/100,000), had a peak of percentage of cases hospitalized in ICU which did not exceed 79.4% and a mortality<0.27/1,000. On the other hand, in regions with higher incidence at lockdown, CI reached 382–921 cases/100,000, the peak of percentage of cases hospitalized in ICU and mortality rate reached 270%, and 1.5/1,000, respectively. Our data suggests that level of CI at the moment of lockdown is important to control the subsequent spread of infection so NPMs should be adopted very early during the course of Covid-19 epidemic, in order to mitigate the impact on the healthcare system and to reduce related mortality.
Safety of antidepressants in a primary care cohort of adults with obesity and depression
Authors: Morriss Richard , Tyrer Freya , Zaccardi Francesco , Khunti Kamlesh
Published: January 2021

Background

Obesity, depressive disorders and antidepressant drugs are associated with increased mortality, cardiovascular disease, diabetes, fractures and falls. We explored outcomes associated with the most commonly prescribed antidepressants in overweight or obese people with depression.

Methods and findings

We identified a cohort of overweight or obese adults (≥18 years) in primary care from the UK Clinical Practice Research Datalink, linked with hospital and mortality data, between 1 January 2000 and 31 December 2016 who developed incident depression to January 2019. Cox proportional hazards models and 99% confidence intervals were used to estimate hazard ratios (HR) for mortality, cardiovascular disease, diabetes, and falls/fractures associated with exposure to selective serotonin reuptake inhibitors (SSRIs), tricyclic (TCA)/other, combination antidepressants, citalopram, fluoxetine, sertraline, amitriptyline and mirtazapine, adjusting for potential confounding variables. In 519,513 adults, 32,350 (9.2 per 1,000 years) displayed incident depression and 21,436 (66.3%) were prescribed ≥1 antidepressant. Compared with no antidepressants, all antidepressant classes were associated with increased relative risks of cardiovascular disorders [SSRI HR: 1.32 (1.14–1.53), TCA/Other HR: 1.26 (1.01–1.58)], and diabetes (any type) [SSRI HR: 1.28 (1.10–1.49), TCA/Other: 1.52 (1.19–1.94)]. All commonly prescribed antidepressants except citalopram were associated with increased mortality compared with no antidepressants. However, prescription ≥1 year of ≥40mg citalopram was associated with increased mortality and falls/fractures and ≥1 year 100mg sertraline with increased falls/fractures.

Conclusions

In overweight/obese people with depression, antidepressants may be overall and differentially associated with increased risks of some adverse outcomes. Further research is required to exclude indication bias and residual confounding.
Effectiveness of intraprocedural dual-phase cone-beam computed tomography in detecting hepatocellular carcinoma and improving treatment outcomes following conventional transarterial chemoembolization
Authors: Cho Youngjong , Lee Sangjoon , Park Sung-Joon
Published: January 2021

To investigate the effectiveness of intraprocedural dual-phase cone-beam computed tomography (CBCT) in detecting hepatocellular carcinoma (HCC) during conventional transcatheter arterial chemoembolization (TACE) and its effect on improving treatment outcomes. Between November 2018 and November 2019, data from 111 patients with unresectable HCCs (N = 263 lesions) were reviewed retrospectively. All patients had undergone baseline magnetic resonance imaging (MRI) scans within one month prior to the procedure. Both arterial-phase (AP) and delayed-phase CBCT images were acquired during all conventional TACEs. Each HCC detection rate when read by AP-CBCT and when read by dual-phase (DP) CBCT including both AP and delayed phase was compared with that of MRI, and the diagnosis of HCC was based on MRI. Additionally, the follow-up results concerning lipiodol uptake status and tumor response of the lesions detected only by AP-/DP-CBCT were analyzed and compared with MRI-only detected lesions. The overall sensitivity of DP-CBCT (94.7%) was significantly higher than that of AP-CBCT (89.0%) (p = 0.003). In particular, the rate of subcentimeter HCC detection by DP-CBCT was pronounced (91.5% vs. 80.3%) (p = 0.01). Lesions found only by DP-CBCT exhibited positive lipiodol uptake (n = 31/31; 100%) and showed complete or partial responses (n = 24/31; 77.4%) on follow-up CT imaging, while MRI-only detected lesions had less lipiodol uptake (n = 6/14, 42.9%) and complete or partial responses (n = 4/14; 28.6%) (p ≤ 0.001). DP-CBCT imaging during TACE enabled better detection of HCCs than when using AP-CBCT alone, and AP- and DP-CBCT is superior to MRI in detecting chemoembolization-sensitive lesions. This resulted in increased detectability of HCCs and the achievement of better treatment outcomes.
Testing the causal mechanism of the peninsular effect in passerine birds from South Korea
Authors: Kim Jin-Yong , Shin Man-Seok , Seo Changwan , Eo Soo Hyung , Hong Seungbum
Published: January 2021

The peninsular effect is a geographical phenomenon that explains patterns of species richness. Given that spatial variation in species richness along a peninsular may be driven by multiple processes, we aimed to identify the sources of latitudinal patterns in passerine species richness and test hypotheses regarding (1) recent deterministic processes (climate, primary productivity, forest area, and habitat diversity), (2) anthropogenic processes (habitat fragmentation), and (3) stochastic processes (migration influence) in the Korean peninsula. We used the distribution data of 147 passerine species from 2006 to 2012. Single regression between passerine species richness and latitude supported the existence of the peninsular effect. Mean temperature increased with decreasing latitude, as did habitat diversity but leaf area index and forest area decline. However, mean temperature and forest area only influenced passerine species richness. Although habitat diversity influenced passerine species richness, it was counter to the expectations associated with peninsular effect. The number of habitat patches decreased as latitude increased but it had no effect on passerine species richness. Ratio of migrant species richness showed no significant relationship with leaf area index, forest area, and habitat diversity. However, the ratio of migrant species richness increased with decreasing mean temperature and that contributed to the increase in passerine species. Overall, our finding indicate that the observed species richness pattern in peninsulas with the tip pointing south (in the northern hemisphere) counter to the global latitudinal gradient. These results were caused by the peninsular effect associated with complex mechanism that interact with climate, habitat area, and migrant species inflow.
Genome-wide association analysis of Mexican bread wheat landraces for resistance to yellow and stem rust
Authors: Vikram Prashant , Sehgal Deepmala , Sharma Achala , Bhavani Sridhar , Gupta Priyanka , Randhawa Mandeep , Pardo Neftali , Basandra Daisy , Srivastava Puja , Singh Sanjay , Sood Tanvi , Sansaloni Carolina Paola , Rahman Hifzur , Singh Sukhwinder
Published: January 2021

Deploying under-utilized landraces in wheat breeding has been advocated to accelerate genetic gains in current era of genomics assisted breeding. Mexican bread wheat landraces (Creole wheats) represent an important resource for the discovery of novel alleles including disease resistance. A core set of 1,098 Mexican landraces was subjected to multi-location testing for rust diseases in India, Mexico and Kenya. The landrace core set showed a continuous variation for yellow (YR) and stem rust (SR) disease severity. Principal component analysis differentiated Mexican landraces into three groups based on their respective collection sites. Linkage disequilibrium (LD) decay varied from 10 to 32 Mb across chromosomes with an averge of 23Mb across whole genome. Genome-wide association analysis revealed marker-trait associations for YR resistance in India and Mexico as well as for SR resistance in Kenya. In addition, significant additive-additive interaction effects were observed for both YR and SR resistance including genomic regions on chromosomes 1BL and 3BS, which co-locate with pleiotropic genes Yr29/Lr46/Sr58/Pm39/Ltn2 and Sr2/Yr30/Lr27, respectively. Study reports novel genomic associations for YR (chromosomes 1AL, 2BS, and 3BL) and SR (chromosomes 2AL, 4DS, and 5DS). The novel findings in Creole wheat landraces can be efficiently utilized for the wheat genetic improvement.
Factors associated with hypertension in Pakistan: A systematic review and meta-analysis
Authors: Riaz Muhammad , Shah Ghazala , Asif Muhammad , Shah Asma , Adhikari Kaustubh , Abu-Shaheen Amani
Published: January 2021

Background

High blood pressure is an important public health concern and the leading risk factor for global mortality and morbidity. To assess the implications of this condition, we aimed to review the existing literature and study the factors that are significantly associated with hypertension in the Pakistani population.

Methods

We conducted several electronic searches in PubMed, ISI Web of Science, PsycINFO, EMBASE, Scopus, Elsevier, and manually searched the citations of published articles on hypertension from May 2019 to August 2019. We included all studies that examined factors associated with hypertension regardless of the study design. To assess the quality of the research, we used the Newcastle-Ottawa Quality Assessment Scale. We also conducted meta-analyses using the DerSimonian & Laird random-effects model to collate results from at least three studies.

Results

We included 30 cross-sectional and 7 case-control studies (99,391 participants country-wide) in this review and found 13 (35.1%) to be high-quality studies. We identified 5 socio-demographic, 3 lifestyle, 3 health-related, and 4 psychological variables that were significantly associated with hypertension. Adults aged between 30–60 years who were married, living in urban areas with high incomes, used tobacco, had a family history of hypertension, and had comorbidities (overweight, obesity, diabetes, anxiety, stress, and anger management issues) were positively associated with hypertension. On the other hand, individuals having high education levels, normal physical activity, and unrestricted salt in their diet were negatively associated with hypertension.

Conclusion

We found several socio-demographic, lifestyle, health-related, and psychological factors that were significantly (positively and negatively) associated with hypertension. Our findings may help physicians and public health workers to identify high-risk groups and recommend appropriate prevention strategies. Further research is warranted to investigate these factors rigorously and collate global evidence on the same.
Late initiation of antenatal care and associated factors among pregnant women attending antenatal clinic of Ilu Ababor Zone, southwest Ethiopia: A cross-sectional study
Authors: Tola Waqgari , Negash Efrem , Sileshi Tesfaye , Wakgari Negash
Published: January 2021

Timely entries to antenatal care have various benefits for pregnant women and birth outcomes. The aim of antenatal care is to assure that every pregnancy culminates in the delivery of a healthy baby without negative effects on the health of pregnant women through health promotion and disease prevention, early detection, and treatment of complications and existing diseases. Hence, this study assessed the late initiation of antenatal care and associated factors among pregnant women attending antenatal clinics at public health centers of Ilu Ababor Zone, southwest Ethiopia. An Institution-based cross-sectional study was carried out among 389 pregnant women who were attending antenatal care service at twelve randomly selected health centers. A systematic sampling technique was employed to recruit pregnant women. Pretested and structured questionnaires were used to collect data. Data were entered into Epidata and exported to SPSS for analysis. Those women who started antenatal care follow up after 12 weeks of gestational age were categorized as booked lately. Bivariable and multivariable logistic regression was employed to identify an association between the independent predictors and the outcome variable. In this study, 277 (71.2%) of the participants were booked their first antenatal care visit lately. Having family size of ≥ 4 (AOR: 2.25; 95% CI: 1.07–4.74), maternal age ≥ 25 years (AOR: 2.30; 95%CI: 1.02–5.18) and perceived the right time of booking > 12 weeks of gestation (AOR: 2.39; 95% CI: 1.13–5.04) had higher odds of late antenatal care initiation. Similarly, not being a member of women’s health developmental army (AOR: 2.35; 95%CI: 1.09–5.07) and ANC not attended previously (AOR: 3.32; 95% CI: 1.17–9.42) had also a more likelihood of booking antenatal care lately. In this study, the majority of women started antenatal care lately. Thus, the provision of health education on the importance of attending first antenatal care early is recommended.
Professional competencies and public health content in the human nutrition and dietetics degree program: A qualitative study based on experts' consensus
Authors: Soares Panmela , Vives-Cases Carmen , Clemente-Gómez Vicente , Ortiz-Moncada Rocío , Lobo-Escolar Elena , Rada-Fernández de Jauregui Diego , Arija Victoria , Zapata-Moya Ángel R. , Davó-Blanes Mari Carmen ,   Group of participants in the Fifth Meeting of University Public Health Professors of the Degree in Human Nutrition and Dietetics
Published: January 2021

Objective

To gather consensus on professional competencies and basic public health content for the degree program in Human Nutrition and Dietetics (HND).

Design

In 2018, the Fifth Meeting of University Public Health Professors took place in Zaragoza (Spain). Fourteen lecturers in the HND degree program participated from 11 Spanish universities. They identified competencies and basic content for training for the HND degree using group dynamics and consensus strategies.

Results

The professors identified 51 basic competencies, distributed in the areas of “evaluation of population health needs” (n = 20), “development of health policies” (n = 23), and “guaranteeing provision of health care services” (n = 8). In order to reach these competencies, 35 topics were proposed organized into six thematic blocks: foundations of public health, nutritional epidemiology, health problems and diet and nutrition strategies, food security, health in all policies and health promotion and education.

Conclusion

The consensus reached serves as a reference to orient and update public health education as a part of the HND degree.
Physical activity, sedentary behavior and functionality in older adults: A cross-sectional path analysis
Authors: Garcia Meneguci Cíntia Aparecida , Meneguci Joilson , Sasaki Jeffer Eidi , Tribess Sheilla , Júnior Jair Sindra Virtuoso
Published: January 2021

Disability is negatively associated with the health of older adults, and it can be mediated by healthy lifestyles and behaviors throughout one’s life. In this context, understanding the interrelationships between sedentary behavior, physical activity and functionality may assist in the implementation of effective public health actions. Thus, the aim of the present study was to investigate the relationships between both physical activity and sedentary behavior and functionality in older adults and the possible mediators. The variables analyzed were selected according to the content analysis of International Classification of Functioning, Disability and Health model, and included activity, participation, health conditions, body functions and structures, environmental factors and personal factors. 419 individuals participated in the study. Physical activity was directly associated with disability in instrumental activities of daily living (IADL), and the association was mediated by self-esteem, aerobic endurance, and agility/balance. Sedentary behavior was indirectly associated with IADL disability, and the association was mediated by aerobic resistance, nutritional status, and agility/balance. Regarding the basic activities of daily living (BADL), physical activity showed an indirect association mediated by aerobic resistance and IADL. The association of sedentary behavior with BADL was mediated by aerobic resistance and lower limb flexibility. These results reinforce the idea that functionality is multidimensional, and the mediating factors must be considered when strategies for promoting physical activity and reducing sedentary behavior are designed.
Exploration of metabolite profiles in the biofluids of dairy cows by proton nuclear magnetic resonance analysis
Authors: Kim Hyun Sang , Kim Eun Tae , Eom Jun Sik , Choi You Young , Lee Shin Ja , Lee Sang Suk , Chung Chang Dae , Lee Sung Sill
Published: January 2021

Studies that screen for metabolites produced in ruminants are actively underway. We aimed to evaluate the metabolic profiles of five biofluids (ruminal fluid, serum, milk, urine, and feces) in dairy cow by using proton nuclear magnetic resonance (1H-NMR) and provide a list of metabolites in each biofluid for the benefit of future research. We analyzed the metabolites in five biofluids from lactating cows using proton nuclear magnetic resonance imaging; 96, 73, 88, 118, and 128 metabolites were identified in the five biofluids, respectively. In addition, 8, 6, 9, and 17 metabolites were unique to ruminal fluid, serum, milk, and urine, respectively. The metabolites present at high concentrations were: acetate, propionate, and butyrate in ruminal fluid; lactate, glucose, and acetate in serum; and lactose, guanidoacetate, and glucitol in milk. In addition, the following metabolites were present at high concentrations: hippurate, urea, and trimethylamine N-oxide in urine and acetate, propionate, and butyrate in feces. The score plots of the principal component analysis did not show clear distinctions among the five biofluid samples. The purpose of this study was to verify the ability of our metabolomics approaches to identify metabolites in the biofluids of dairy cows.
Peguero-Lo Presti criteria for the diagnosis of left ventricular hypertrophy: A systematic review and meta-analysis
Authors: Yu Zongying , Song Jie , Cheng Li , Li Shasha , Lu Qun , Zhang Yafeng , Lin Xiaoci , Liu Dadong
Published: January 2021

Background

The Peguero-Lo Presti criteria are novel electrocardiographic (ECG) diagnostic criteria for the detection of left ventricular hypertrophy (LVH) and represent the sum of the amplitude of the deepest S wave in any lead with the S wave in lead V4(SD+SV4). The diagnostic efficacy of the Peguero-Lo Presti criteria in LVH is still debatable. We aimed to test the sensitivity and specificity of the Peguero-Lo Presti criteria and compared them with those of the Cornell voltage index to assess their overall performance in LVH diagnosis.

Methods

Electronic databases (e.g., Medline, Web of Knowledge, Embase, and the Cochrane Library) were searched from their inception until May 18, 2020. Trials written in English that investigated the Peguero-Lo Presti criteria for detecting LVH were included. Data were independently extracted and analyzed by two investigators.

Results

A total of 51 records were screened, and 6 trials comprising 13,564 patients were finally included. A bivariate analysis showed that the sensitivity of the Peguero-Lo Presti criteria (0.52, 95% confidence interval (CI) 0.46–0.58) was higher than that of the Cornell voltage index (0.29, 95% CI 0.23–0.36) and Sokolow-Lyon criteria (0.24, 95% CI 0.21–0.27); the diagnostic accuracy of the Peguero-Lo Presti criteria (0.69, 95% CI 0.65–0.73) was also higher than that of the Cornell voltage index (0.67, 95% CI 0.62–0.71) and Sokolow-Lyon criteria (0.28, 95% CI 0.25–0.32); and the specificity of the Peguero-Lo Presti criteria (0.85, 95% CI 0.79–0.90) was similar to that of the Cornell voltage index (0.92, 95% CI 0.89–0.95) and Sokolow-Lyon criteria (0.94, 95%CI 0.88–0.97). Two trials (including 12,748 patients) were discharged because they included partly healthy subjects and accounted for substantial heterogeneity. Pooled analysis of the remaining 4 trials (including 816 patients) showed that the sensitivity of the Peguero-Lo Presti criteria (0.56, 95% CI 0.51–0.61) was also higher than that of the Cornell voltage index (0.36, 95% CI 0.31–0.42) and Sokolow-Lyon criteria (0.24, 95% CI 0.18–0.31); the diagnostic accuracy of the Peguero-Lo Presti criteria (0.84, 95% CI 0.80–0.87) was also higher than that of the Cornell voltage index (0.54, 95% CI 0.50–0.58) and Sokolow-Lyon criteria (0.38, 95% CI 0.34–0.42); and the specificity of the Peguero-Lo Presti criteria (0.90, 95% CI 0.87–0.92) was similar to that of the Cornell voltage index (0.93, 95% CI 0.88–0.96) and Sokolow-Lyon criteria (0.97, 95% CI 0.90–0.99). Both the likelihood ratio and posttest probability of the Peguero-Lo Presti criteria and Cornell voltage index were moderate.

Conclusion

Based on this systematic review and meta-analysis, the Peguero-Lo Presti criteria-based ECG diagnostic method for LVH has high sensitivity, specificity and diagnostic accuracy and should be applied in clinical practice settings.
Comparison of blue-green solutions for urban flood mitigation: A multi-city large-scale analysis
Authors: Cristiano Elena , Farris Stefano , Deidda Roberto , Viola Francesco
Published: January 2021

Flooding risk in cities has been recently exacerbated by increased urbanization and climate change, often with catastrophic consequences in terms of casualties and economic losses. Rainwater harvesting systems and green roofs are recognized as being among the most effective blue-green mitigation measures. However, performances of these systems have currently been investigated only at laboratory or very-small local scales. In this work, we assess the potential benefit of the extensive installation of these solutions on all the rooftops of 9 cities, with different climatological and geographical characteristics. Both surface discharge reduction and delay between rainfall and runoff peak generation have been investigated. Green roofs ensure a larger average lag time between rainfall and runoff peaks than rainwater harvesting systems, without significant differences between intensive and extensive structures. On the other hand, the cost-efficiency analysis, considering the entire urban area, shows a higher retention capacity with a lower financial investment for rainwater harvesting rather than for green roofs in most cases. For extreme rainfall events, large-scale installation of rainwater harvesting systems coupled with intensive green roofs over the entire city have shown to be the most efficient solution, with a total discharge reduction that can vary from 5% to 15%, depending on the city characteristics and local climate.
Human FcRn expression and Type I Interferon signaling control Echovirus 11 pathogenesis in mice
Authors: Wells Alexandra I. , Grimes Kalena A. , Kim Kenneth , Branche Emilie , Bakkenist Christopher J. , DePas William H. , Shresta Sujan , Coyne Carolyn B.
Published: January 2021

Neonatal echovirus infections are characterized by severe hepatitis and neurological complications that can be fatal. Here, we show that expression of the human homologue of the neonatal Fc receptor (hFcRn), the primary receptor for echoviruses, and ablation of type I interferon (IFN) signaling are key host determinants involved in echovirus pathogenesis. We show that expression of hFcRn alone is insufficient to confer susceptibility to echovirus infections in mice. However, expression of hFcRn in mice deficient in type I interferon (IFN) signaling, hFcRn-IFNAR-/-, recapitulate the echovirus pathogenesis observed in humans. Luminex-based multianalyte profiling from E11 infected hFcRn-IFNAR-/- mice revealed a robust systemic immune response to infection, including the induction of type I IFNs. Furthermore, similar to the severe hepatitis observed in humans, E11 infection in hFcRn-IFNAR-/- mice caused profound liver damage. Our findings define the host factors involved in echovirus pathogenesis and establish in vivo models that recapitulate echovirus disease in humans.
Awjedni: A Reverse-Image-Search Application
Authors: Al-Lohibi Hanaa , Alkhamisi Tahani , Assagran Maha , Aljohani Amal , Aljahdali Asia Othaman
Published: January 2021

The abundance of photos on the internet, along with smartphones that could implement computer vision technologies allow for a unique way to browse the web. These technologies have potential used in many widely accessible and globally available reverse-image search applications. One of these applications is the use of reverse-image search to help people finding items which they're interested in, but they can’t name it. This is where Awjedni was born. Awjedni is a reverse-image search application compatible with iOS and Android smartphones built to provide an efficient way to search millions of products on the internet using images only. Awjedni utilizes a computer vision technology through implementing multiple libraries and frameworks to process images, recognize objects, and crawl the web. Users simply upload/take a photo of a desired item and the application returns visually similar items and a direct link to the websites that sell them.
An access control and authorization model with Open stack cloud for Smart Grid
Authors: Rathod Yagnik A
Published: January 2021

In compare to Authentication for identification and relationship of an identity of a user with its task and process within the system, authorization in access control is much anxious about confirming that user and its task in the form of system process, access to the assets of any particular domain is only approved when proven obedient to the identified policies. Access control and authorization is always an area of interest for researchers for enhancing security of critical assets from many decades. Our prime focus and interest is in the field of access control model based on Attribute base access control (ABAC) and with this paper we tried to integrate ABAC with openstack cloud for achieving finer level of granularity in access policies for domain like smart grid. Technical advancement of current era demands that critical infrastructure like traditional electrical grid open ups to the modern information and communication technology to get the benefit in terms of efficiency, scalability, accessibility and transparency for better adaptability in real world. Incorporation of ICT with electric grid makes it possible to do greater level of bi-directional interaction among stake holders like customer, generation units, distribution units and administrations and these leads international organization to contribute for standardization of smart grid concepts and technology so that the realization of smart grid becomes reality. Smart grid is a distributed system of very large scale by its nature and needs to integrate available legacy systems with its own security requirements. Cloud computing proven to be most efficient approach for said requirements and we have identified openstack as our cloud platform. We have integrated ABAC approach with default RBAC approach of openstack and provide a frame work that supports and integrate multiple access control polices in making authorization decisions. Smart grid domain in considered as case study which requires support of multiple access policies (RBAC, ABAC or DAC etc) with our model for access control and authorization.
Análisis tecnotipológico y cronológico de la indústria lítica superficial recuperada en las terrazas del cauce del bajo Ebro
Authors: Gironès Rofes Ivan , Molist Montaña Miquel , Pardo Gordó Salvador
Published: January 2021

Los estudios focalizados en la Prehistoria Reciente peninsular han sido escasos en el cauce bajo del valle del Ebro (Tarragona). La investigación en esta área se caracteriza sobre todo por el estudio de los materiales recuperados en intervenciones arqueológicas de urgencia o bien en su inventario. Esto deriva en la emergencia de una problemática definida por una continua ausencia de interpretaciones válidas respecto a las dinámicas ocupacionales del territorio. Para poder analizar las dinámicas ocupacionales y cronoculturales, en este trabajo se presenta el análisis y estudio de varios conjuntos líticos procedentes de prospecciones no sistemáticas de cuatro áreas −Vall del Llop, La Conca, Vall de Mantons y Vall de Carrinya− desde una perspectiva morfo-tecno-tipológica. Se pretende poder establecer estimaciones cronológicas de los conjuntos y combinarlas con la observación off-site. Para ello el enfoque se basa en la aplicación de técnicas de modelización bayesiana que puedan aproximarnos al contexto espaciotemporal de los artefactos analizados.
El prestigio de los objetos: mangos de marfil en el mundo ibérico
Authors: Mata Parreño Consuelo , Soria Combadiera Lucía , Blasco Martín Marta , Gallello Gianni , Belarte Maria Carme , Pastor García Agustín
Published: January 2021

El estudio se centra en una serie de mangos de marfil de características morfotécnicas similares, recuperados en espacios de hábitat y funerarios ibéricos y datados entre el s. IV y el s. II a. C. Se trata de objetos de prestigio al alcance de una élite aristocrática, que han sido elaborados con materias primas exóticas (marfil) y técnicas decorativas inusuales que indican una especialización. A través de análisis de composición por infrarrojo cercano por Transformada de Fourier (FT-NIR) y de fluorescencia de rayos X (pXRF) se ha podido documentar la presencia de incrustaciones de ámbar adheridas a la superficie de los mangos mediante el uso del estaño como soldadura blanda y el empleo de apliques metálicos de bronce y plata. Se debate sobre la procedencia de estas piezas y sobre el conocimiento técnico artesanal necesario para fabricarlas.
Unique Case of a Hamartomatous Duodenal Polyp Associated With Intestinal Schistosomiasis
Authors: Gonzalez Adalberto , Gupta Kapil , Rodriguez Sandra , Wadhwa Vaibhav , Bejarano Pablo , Charles Roger
Published: January 2021

Schistosomiasis is a trematode infection rarely diagnosed in the United States. Intestinal involvement is common with chronic infection and causes abdominal pain, changes in bowel habits, hematochezia, and polyp formation. Chronic, disseminated infection can affect the intestines causing the aforementioned symptoms, but reports of intestinal polyps are rare. Most cases are inflammatory fibrous polyps in the colon. There are very few cases reported in the literature of hamartomatous polyps arising in the small intestine. We present the rare case of a U.S.-born, 35-year-old woman diagnosed with a large duodenal hamartomatous polyp in the setting of intestinal schistosomiasis.
Spontaneous Rupture of Gallbladder Hemangioma Presenting as Hemoperitoneum
Authors: Ray Choudhury Satyaprakash , Mohanty Sumit Subhadarshi , Pattanaik Premananda , Lenka Anasuya , Panda Bhagaban
Published: January 2021

Hemangioma of the gallbladder is rare, with less than 10 cases reported in the literature. It may represent a hamartomatous proliferation of connective tissue in the gallbladder or may be congenital in origin. Although liver hemangiomas rarely present with spontaneous rupture, it has not been documented in gallbladder hemangiomas. This report presents a case of gallbladder hemangioma that ruptured spontaneously and presented with hemoperitoneum.