bluuebunny/crossref_metadata_2025 huggingface dataset:
The Complete Mitochondrial Genome and Gene Arrangement of the Enigmatic Scaphopod Pictodentalium vernedei
Authors: Zhang Tianzhe , Wang Yunan , Song Hao
Published: January 2023

The enigmatic scaphopods, or tusk shells, are a small and rare group of molluscs whose phylogenomic position among the Conchifera is undetermined, and the taxonomy within this class also needs revision. Such work is hindered by there only being a very few mitochondrial genomes in this group that are currently available. Here, we present the assembly and annotation of the complete mitochondrial genome from Dentaliida Pictodentalium vernedei, whose mitochondrial genome is 14,519 bp in size, containing 13 protein-coding genes, 22 tRNA genes and two rRNA genes. The nucleotide composition was skewed toward A-T, with a 71.91% proportion of AT content. Due to the mitogenome-based phylogenetic analysis, we defined P. vernedei as a sister to Graptacme eborea in Dentaliida. Although a few re-arrangements occurred, the mitochondrial gene order showed deep conservation within Dentaliida. Yet, such a gene order in Dentaliida largely diverges from Gadilida and other molluscan classes, suggesting that scaphopods have the highest degree of mitogenome arrangement compared to other molluscs.
Potential Involvement of LncRNAs in Cardiometabolic Diseases
Authors: Ilieva Mirolyuba , Uchida Shizuka
Published: January 2023

Characterized by cardiovascular disease and diabetes, cardiometabolic diseases are a major cause of mortality around the world. As such, there is an urgent need to understand the pathogenesis of cardiometabolic diseases. Increasing evidence suggests that most of the mammalian genome are transcribed as RNA, but only a few percent of them encode for proteins. All of the RNAs that do not encode for proteins are collectively called non-protein-coding RNAs (ncRNAs). Among these ncRNAs, long ncRNAs (lncRNAs) are considered as missing keys to understand the pathogeneses of various diseases, including cardiometabolic diseases. Given the increased interest in lncRNAs, in this study, we will summarize the latest trend in the lncRNA research from the perspective of cardiometabolism and disease by focusing on the major risk factors of cardiometabolic diseases: obesity, cholesterol, diabetes, and hypertension. Because genetic inheritance is unavoidable in cardiometabolic diseases, we paid special attention to the genetic factors of lncRNAs that may influence cardiometabolic diseases.
Genome-Wide Identification of GYF-Domain Encoding Genes in Three Brassica Species and Their Expression Responding to Sclerotinia sclerotiorum in Brassica napus
Authors: Zhang Xiaobo , Qin Lei , Lu Junxing , Xia Yunong , Tang Xianyu , Lu Xun , Xia Shitou
Published: January 2023

GYF (glycine-tyrosine-phenylalanine)-domain-containing proteins, which were reported to participate in many aspects of biological processes in yeast and animals, are highly conserved adaptor proteins existing in almost all eukaryotes. Our previous study revealed that GYF protein MUSE11/EXA1 is involved in nucleotide-binding leucine-rich repeat (NLR) receptor-mediated defense in Arabidopsis thaliana. However, the GYF-domain encoding homologous genes are still not clear in other plants. Here, we performed genome-wide identification of GYF-domain encoding genes (GYFs) from Brassica napus and its parental species, Brassica rapa and Brassica oleracea. As a result, 26 GYFs of B. napus (BnaGYFs), 11 GYFs of B. rapa (BraGYFs), and 14 GYFs of B. oleracea (BolGYFs) together with 10 A. thaliana (AtGYFs) were identified, respectively. We, then, conducted gene structure, motif, cis-acting elements, duplication, chromosome localization, and phylogenetic analysis of these genes. Gene structure analysis indicated the diversity of the exon numbers of these genes. We found that the defense and stress responsiveness element existed in 23 genes and also identified 10 motifs in these GYF proteins. Chromosome localization exhibited a similar distribution of BnaGYFs with BraGYFs or BolGYFs in their respective genomes. The phylogenetic and gene collinearity analysis showed the evolutionary conservation of GYFs among B. napus and its parental species as well as Arabidopsis. These 61 identified GYF domain proteins can be classified into seven groups according to their sequence similarity. Expression of BnaGYFs induced by Sclerotinia sclerotiorum provided five highly upregulated genes and five highly downregulated genes, which might be candidates for further research of plant–fungal interaction in B. napus.
Full-Length Transcriptome Profiling of Coridius chinensis Mitochondrial Genome Reveals the Transcription of Genes with Ancestral Arrangement in Insects
Authors: Xu Shiwen , Duan Yuange , Ma Ling , Song Fan , Tian Li , Cai Wanzhi , Li Hu
Published: January 2023

Coridius chinensis (Hemiptera: Dinidoridae) is a medicinal insect. Its mitochondrial gene arrangement is consistent with that of Drosophila melanogaster and Erthesina fullo, the two insects with well-studied mitochondrial transcription. To investigate whether the structural consistency of mitochondrial genes leads to similarities in transcription and post-transcriptional processing, we improved the gene annotation and constructed a quantitative transcription map for the C. chinensis mitochondrial genome (mitogenome) using full-length transcriptome sequencing. The size of this mitogenome was 16,214 bp and the proposed model of mitochondrial transcription was similar to that of Drosophila. Both strands were nearly entirely transcribed except for the antisense genes downstream of trnS2 on N strand. The expression of cytochrome c subunit genes is higher than that of NADH-dehydrogenase subunit genes. The post-transcriptional cleavage process followed the “tRNA punctuation” model, and both the “reverse cleavage” model in Drosophila and “forward cleavage” model in E. fullo were found in C. chinensis. In addition, we found that long non-coding RNAs from the control region contained tandem repeats. Polyadenylation was performed after CCA triplet at the 3′ end of tRNA. The isoform diversity of lrRNA was identified. Our study sheds light on the transcriptional regulation and RNA processing of insect mitogenomes with the putative ancestral gene arrangement.
The Complete Chloroplast Genome of An Ophiorrhiza baviensis Drake Species Reveals Its Molecular Structure, Comparative, and Phylogenetic Relationships
Authors: Pham Mai Huong , Tran Thu Hoai , Le Thi Dung , Le Tung Lam , Hoang Ha , Chu Hoang Ha
Published: January 2023

Ophiorrhiza baviensis Drake, a flowering medical plant in the Rubiaceae, exists uncertainly within the Ophiorrhiza genus’ evolutionary relationships. For the first time, the whole chloroplast (cp) genome of an O. baviensis Drake species was sequenced and annotated. Our findings demonstrate that the complete cp genome of O. baviensis is 154,770 bp in size, encoding a total of 128 genes, including 87 protein-coding genes, 8 rRNAs, and 33 tRNAs. A total of 59 SSRs were screened in the studied cp genome, along with six highly variable loci, which can be applied to generate significant molecular markers for the Ophiorrhiza genus. The comparative analysis of the O. baviensis cp genome with two published others of the Ophiorrhiza genus revealed a high similarity; however, there were some notable gene rearrangements in the O. densa plastome. The maximum likelihood phylogenetic trees were constructed based on the concatenation of the rps16 gene and the trnL-trnF intergenic spacer sequence, indicating a close relationship between the studied O. baviensis and other Ophiorrhiza. This study will provide a theoretical molecular basis for identifying O. baviensis Drake, as well as species of the Ophiorrhiza genus, and contribute to shedding light on the chloroplast genome evolution of Rubiaceae.
Metatranscriptomic Analyses Reveal Important Roles of the Gut Microbiome in Primate Dietary Adaptation
Authors: Zhang Mingyi , Wang Xiaochen , Wang Ziming , Mao Shuxin , Zhang Jiali , Li Ming , Pan Huijuan
Published: January 2023

The gut microbiome plays a vital role in host ecological adaptation, especially dietary adaptations. Primates have evolved a variety of dietary and gut physiological structures that are useful to explore the role of the gut microbiome in host dietary adaptations. Here, we characterize gut microbiome transcriptional activity in ten fecal samples from primates with three different diets and compare the results to their previously reported metagenomic profile. Bacteria related to cellulose degradation, like Bacteroidaceae and Alcaligenaceae, were enriched and actively expressed in the gut microbiome of folivorous primates, and functional analysis revealed that the glycan biosynthesis and metabolic pathways were significantly active. In omnivorous primates, Helicobacteraceae, which promote lipid metabolism, were significantly enriched in expression, and activity and xenobiotic biodegradation and metabolism as well as lipid metabolism pathways were significantly active. In frugivorous primates, the abundance and activity of Elusimicrobiaceae, Neisseriaceae, and Succinivibrionaceae, which are associated with digestion of pectin and fructose, were significantly elevated, and the functional pathways involved in the endocrine system were significantly enriched. In conclusion, the gut microbiome contributes to host dietary adaptation by helping hosts digest the inaccessible nutrients in their specific diets.
Genetic and Morphological Variation in Hypodontia of Maxillary Lateral Incisors
Authors: Kerekes-Máthé Bernadette , Mártha Krisztina , Bănescu Claudia , O’Donnell Matthew Brook , Brook Alan H.
Published: January 2023

(1) Background: Hypodontia has a multifactorial aetiology, in which genetic factors are a major component. Associated with this congenital absence, the formed teeth may show differences in size and shape, which may vary with the specific genetic variants and with the location of the missing teeth. The aims of the present study were to investigate a specific variant of MSX1, derive morphometric tooth measurements in a sample of patients with isolated maxillary lateral incisor agenesis and matched controls, and model the findings. (2) Methods: Genotyping of the MSX1 rs8670 genetic variant and morphometric measurements with a 2D image analysis method were performed for 26 hypodontia patients and 26 matched controls. (3) Results: The risk of upper lateral incisor agenesis was 6.9 times higher when the T allele was present. The morphometric parameters showed significant differences between hypodontia patients and controls and between the unilateral and bilateral agenesis cases. The most affected crown dimension in the hypodontia patients was the bucco-lingual dimension. In crown shape there was significant variation the Carabelli trait in upper first molars. (4) Conclusions: The MSX1 rs8670 variant was associated with variations in morphological outcomes. The new findings for compensatory interactions between the maxillary incisors indicate that epigenetic and environmental factors interact with this genetic variant. A single-level directional complex interactive network model incorporates the variations seen in this study.
Clinical Evaluation of BioFire COVID-19 Test, BioFire Respiratory Panel 2.1, and Cepheid Xpert Xpress SARS-CoV-2 Assays for Sample-to-Answer Detection of SARS-CoV-2
Authors: Park Joonhong , Kim So Yeon , Lee Jaehyeon , Hong Ki Ho
Published: January 2023

Background: Due to the extreme infectivity of SARS-CoV-2, sample-to-answer SARS-CoV-2 reverse transcription (RT) polymerase chain reaction (PCR) assays are urgently needed in order to facilitate infectious disease surveillance and control. The purpose of this study was to evaluate three sample-to-answer SARS-CoV-2 RT-PCR assays—BioFire COVID-19 Test, BioFire RP 2.1, and Cepheid Xpert Xpress SARS-CoV-2—using clinical samples. Methods: A total of 77 leftover nasopharyngeal swab (NP) swabs (36 positives and 41 negatives) confirmed by reference SARS-CoV-2 RT real-time (q) PCR assay were collected. The clinical sample concordance, as specified by their respective emergency use authorizations (EUAs), in comparison to the reference SARS-CoV-2 RT-qPCR assay, was assessed. Results: The results showed that all three sample-to-answer SARS-CoV-2 RT-PCR assays provided perfectly concordant results consistent with the reference SARS-CoV-2 RT-qPCR assay. The BioFire COVID-19 Test exhibited the best turnaround time (TAT) compared to the other assays, regardless of the test results, using one-way analysis of variance followed by Scheffe’s post hoc test (p < 0.001). The Xpert Xpress SARS-CoV-2 showed a shorter average TAT (mean ± standard deviation, 49.9 ± 3.1 min) in the positive samples compared to that (55.7 ± 2.5 min) of the negative samples. Conclusions: Our evaluation demonstrates that the BioFire COVID-19 Test, BioFire RP 2.1, and Cepheid Xpert Xpress SARS-CoV-2 assays compare favorably to the reference SARS-CoV-2 RT-qPCR assay, along with a 100% concordance in assay results for clinical samples and an acceptable analytical performance at their guaranteed limits of detection. The addition of a widely used simultaneous sample-to-answer SARS-CoV-2 RT-PCR assay will contribute to the number of medical laboratories able to test for COVID-19.
Methodological Changes in the Field of Paleogenetics
Authors: Danielewski Mikołaj , Żuraszek Joanna , Zielińska Aleksandra , Herzig Karl-Heinz , Słomski Ryszard , Walkowiak Jarosław , Wielgus Karolina
Published: January 2023

Paleogenetics has significantly changed since its inception almost forty years ago. Initially, molecular techniques available to the researchers offered minimal possibilities for ancient DNA analysis. The subsequent expansion of the scientific tool cabinet allowed for more remarkable achievements, combined has with the newfound popularity of this budding field of science. Finally, a breakthrough was made with the development of next-generation sequencing (NGS) technologies and the update of DNA isolation protocols, through which even very fragmented aDNA samples could be used to sequence whole genomes. In this paper, we review the achievements made thus far and compare the methodologies utilized in this field of science, discussing their benefits and challenges.
Effects of High-Intensity Exercise on Physiological Indicators of Recovery Period by Wearing Face Masks of Elite Athletes
Authors: Kwon Hyeong-Tae , Kim Daeho
Published: January 2023

Athletes need to maintain the necessary physical conditioning for sports performance while wearing face masks to reduce the risk of virus transmission during training and competition during the COVID-19 situation. The quantitative and physiological effects of face masks on exercise capacity needs to be reported. The purpose of this study was to evaluate elite athletes to quantify, in detail, the effect of a KF94 face mask on changes in lactic acid during recovery after high-intensity aerobic exercise. Thirteen elite soft tennis athletes were recruited. A crossover design was used to examine the effects of using a disposable KF94 face mask compared with not masking during exercise. The participants completed a shuttle run test experiment two times during a 3-day period, including 5–10 min of warmup according to their personal preferences. The lactic acid concentration at 20 min of recovery after maximum exercise was 5.98 ± 1.53 mM/L without a mask and 7.61 ± 1.85 mM/L with a KF94 mask (p < 0.001). The maximum laps of shuttle run tests were 101.5 ± 22.5 laps without a mask and 94.2 ± 20.2 laps with a KF94 mask (p < 0.001). Intense exercise after wearing quarantine masks reduces the maximum aerobic exercise ability and decreases the ability to recover lactic acid.
Full Coverage Hourly PM2.5 Concentrations’ Estimation Using Himawari-8 and MERRA-2 AODs in China
Authors: Liu Zhenghua , Xiao Qijun , Li Rong
Published: January 2023

(1) Background: Recognising the full spatial and temporal distribution of PM2.5 is important in order to understand the formation, evolution and impact of pollutants. The high temporal resolution satellite, Himawari-8, providing an hourly AOD dataset, has been used to predict real-time hourly PM2.5 concentrations in China in previous studies. However, the low observation frequency of the AOD due to long-term cloud/snow cover or high surface reflectance may produce high uncertainty in characterizing diurnal variation in PM2.5. (2) Methods: We fill the missing Himawari-8 AOD with MERRA-2 AOD, and drive the random forest model with the gap-filled AOD (AODH+M) and Himawari-8 AOD (AODH) to estimate hourly PM2.5 concentrations, respectively. Then we compare AODH+M-derived PM2.5 with AODH-derived PM2.5 in detail. (3) Results: Overall, the non-random missing information of the Himawari-8 AOD will bring large biases to the diurnal variations in regions with both a high polluted level and a low polluted level. (4) Conclusions: Filling the gap with the MERRA-2 AOD can provide reliable, full spatial and temporal PM2.5 predictions, and greatly reduce errors in PM2.5 estimation. This is very useful for dynamic monitoring of the evolution of PM2.5 in China.
Inconsistent Association between Perceived Air Quality and Self-Reported Respiratory Symptoms: A Pilot Study and Implications for Environmental Health Studies
Authors: Liu Yang , Kwan Mei-Po , Kan Zihan
Published: January 2023

As public awareness of air quality issues becomes heightened, people’s perception of air quality is drawing increasing academic interest. However, data about people’s perceived environment need scrutiny before being used in environmental health studies. In this research, we examine the associations between people’s perceptions of air quality and their self-reported respiratory health symptoms. Spearman rank correlation coefficients were estimated and the associations were tested at the 95% confidence level. Using data collected from participants in two representative communities in Hong Kong, the results indicate a weak but significant association between people’s perceived air quality and their self-reported frequency of respiratory symptoms. However, there are disparities in such an association between different genders, age groups, household income levels, education levels, marital statuses, and geographic contexts. The most striking disparities are between genders and geographic contexts. Multiple significant associations were observed for male participants (correlation coefficients: 0.169~0.205, p-values: 0.021~0.049), while none was observed for female participants. Besides, multiple significant associations were observed in the old town (correlation coefficients: 0.164~0.270, p-values: 0.003~0.048), while none was observed in the new town. The results have significant implications for environmental health research using social media data, whose reliability depends on the association between people’s perceived or actual environments and their health outcomes. Since inconsistent associations exist between different groups of people, researchers need to scrutinize social media data before using them in health studies.
Effect of Insurance Subsidies on Agricultural Land-Use
Authors: Si Chengyu , Li Yanru , Jiang Wei
Published: January 2023

This paper investigates the effect of crop insurance-subsidies on agricultural land-use allocation. Since the objective of crop insurance is to help farmers with risk management, the expected profit from crop production under crop insurance might be improved, leading farmers to allocate more land to crop production. In this paper, agricultural land-use type is classified by irrigated/unirrigated farmland and cropland/woodland/pastureland. The data come from counties from all the continental states. Considering the fractional outcome of land-use share, we apply a multinomial-fractional-logit model to estimate the effects. The results show that insurance subsidies have a significant effect on land-use allocation. An increase in insurance subsidies increases farmland-share, indicating insurance subsidies could be an efficient tool to adjust agricultural land-use allocation.
Structural Relationships between Environmental Factors, Psychological Health, and Academic Performance in Medical Students Engaged in Online Learning during the COVID-19 Pandemic
Authors: Taleb Ola K. , Sarimah Abdullah , Siti-Azrin Ab Hamid , Baharuddin Kamarul Aryffin , Abusafia Ali H.
Published: January 2023

As a result of the COVID-19 outbreak and the enforced quarantine, universities in Malaysia were required to switch to an online class format. The resulting changes in the environmental factors of students may have had an impact on their psychological health and academic performance. This study aimed to determine the effects of environmental factors and the psychological health of students and examine their structural relationship with academic performance. A cross-sectional design with an online self-reported questionnaire was adopted, and the study was conducted among 207 undergraduate medical students at the Health Campus of Universiti Sains Malaysia. The environmental factors were measured using the lighting–noise–temperature scale and technology scale, while psychological health was assessed using the short version of the General Health Questionnaire and academic performance was determined based on Grade Point Average. Descriptive statistics and structural equation modeling were used for analysis of the data. No significant relationship was found between environmental factors and academic performance, or between environmental factors and psychological health. Nonetheless, the hypothesized structural model provided scientific evidence of an inverse relationship between psychological health and academic performance. These findings could be helpful for academics, health policymakers, and health educators in terms of understanding and promoting psychological wellbeing among university students, as well as improving their academic performance.
An Empirical Study of the Contribution of Total Quality Management to Occupational Safety and Health Performance in Saudi Organizations
Authors: Aichouni Mohamed , Touahmia Mabrouk , Alshammari Serhan , Said Mohamed Ahmed , Aichouni Ahmed Baha Eddine , Almudayries Mohsen , Aljohani Hamza
Published: January 2023

Working to ensure a safe and secure work environment for workers and employees has become an essential component of achieving organizational excellence in modern organizations. Occupational safety and health (OSH) programs help in attracting and retaining the workforce and human competencies, thus improving the operational and organizational performance of these organizations. Total quality management (TQM) is a management philosophy adopted by successful organizations to achieve sustainable business performance. This study aims to assess the level of implementation of total quality management and occupational safety and health in Saudi organizations and investigates the relationship between total quality management practices and occupational safety and health performance. Data were collected from a number of random organizations during the period November to December 2021. Based on a sample of 99 valid responses, empirical results were obtained through descriptive and advanced statistical analysis, indicating that TQM practices and OHS are highly implemented in Saudi organizations. The statistical results also showed that TQM practices have a significant positive impact on OSH performance in the surveyed organizations. The seven TQM fundamental pillars and the five OSH program components can be considered as essential success factors and fundamental pillars for TQM implementation in organizations and for OSH performance improvement.
Decoupling between Economic Growth and Carbon Emissions: Based on Four Major Regions in China
Authors: Shen Tao , Hu Runpu , Hu Peilin , Tao Zhang
Published: January 2023

This paper constructs a decoupling model for four major economic regions of China, based on the Tapio decoupling index method and VAR model for carbon emissions to compare and measure the impact of decoupling between carbon emissions and economic growth in China during 1997 to 2019. The results show that the degree of decoupling between economic growth and carbon emissions varies among different economic regions, and the decoupling status is better in all regions at the beginning of the 21st century. In general, the decoupling status in the eastern and western regions is more ideal than that in the central and northeastern regions. The impulse response and variance decomposition results show that renewable energy consumption could always reduce the growth of carbon emission intensity, and its effects are most significant in the western region. The above findings help to reveal the link between economic growth, renewable energy consumption and carbon emissions in China in recent years, and how to ensure a stable economic growth in China while increasing the share of clean energy consumption in each region to achieve carbon neutrality.
Application Effect of MF-OP on Collection of Trivalent Holmium from Rare Earth Mining Wastewater
Authors: Pei Liang , Sun Liying
Published: January 2023

Microtube microfilter with organic phosphoric acid (expressed as MF-OP) containing a wastewater portion with buffer fluid and an enriched portion with nitric acid fluid and organic phosphoric extractant dissolved in benzin has been studied for its trivalent holmium (expressed as Ho(III) collection from rare earth wastewater. Common parameters affecting the collection effect have been investigated, including hydrogen ion molar concentration (molar concentration can be expressed as Cm) or pH value, initial concentration (expressed as Co) of Ho(III), ion-force of rare earth wastewater, voluminal proportion of organic phosphoric extractant with benzin and nitric acid fluid (expressed as Vr), nitric acid Cm, extractant Cm, and type of acid fluid in an enriched portion. The virtues of MF-OP compared to the traditional collection was explored. The impacts of hydrodynamic characteristics (steadiness and current speed) and MF parameter factors (inradius of tube, tube–shell thickness, proportion of holes) on the collection performance of MF-OP for Ho(III) collection were also considered. The test results displayed that the greatest collection conditions of Ho(III) were attained as nitric acid Cm was 4.00 mol/L, extractant Cm was 0.220 mol/L, and Vr was 0.8 in the enriched portion, and pH value was 4.60 in the wastewater portion. Ion- force of rare earth wastewater had no noticeable outcome on Ho(III) collection. The collection proportion of Ho(III) was attainable to 93.1% in 280 min, while Co was 1.80 × 10−3 mol/L.
Effectiveness and Evidence Level of Dance on Functioning of Children and Adolescents with Neuromotor Impairments: A Systematic Review
Authors: Lima Elisangela F. , Brugnaro Beatriz H. , Rocha Nelci Adriana C. F. , Pavão Silvia L.
Published: January 2023

Objectives: The aim of this paper is to investigate the effects of dance therapy in children with neuromotor impairments (CNI), organizing the outcomes according to International Classification of Functioning Disability and Health (ICF) domains, and to investigate if there is adequate evidence of effectiveness to recommend dance as a therapy. Methods: Electronic searches were conducted in December 2021. We include studies assessing the effects of dance in CNI up to 18 years. Data extraction included studies’ populations, intervention features, and main outcomes. We classified outcomes according to the ICF framework. We used the Cochrane collaboration’s tool, modified by effective practice and organization of care (EPOC), to assess the methodological quality. The GRADE synthesized the body of evidence. Results: Twelve studies were included, with most of them addressing the body structure and function and activity components of ICF. Only three studies addressed components of participation, and four of personal factors. All these studies reported the positive effects of dance. Nevertheless, all of them presented high risk of bias. We found very low evidence level for improvement of body structure and function and activity components. Conclusion: Dance therapy presents low evidence level for improvements of body structure and function and activity in CNI. Further studies with low risk of bias and larger samples are needed.
Forecasting Carbon Price Using Double Shrinkage Methods
Authors: Wei Xiaolu , Ouyang Hongbing
Published: January 2023

It is commonly recognized that setting a reasonable carbon price can promote the healthy development of a carbon trading market, so it is especially important to improve the accuracy of carbon price forecasting. In this paper, we propose and evaluate a hybrid carbon price prediction model based on so-called double shrinkage methods, which combines factor screening, dimensionality reduction, and model prediction. In order to verify the effectiveness and superiority of the proposed model, this paper takes data from the Guangdong carbon trading market for empirical analysis. The sample interval is from 5 August 2013 to 25 March 2022. Based on the results of the empirical analysis, several main findings can be summarized. First, the double shrinkage methods proposed in this paper yield more accurate prediction results than various alternative models based on the direct application of factor screening methods or dimensionality reduction methods, when comparing R2, root-mean-square error (RMSE), and root absolute error (RAE). Second, LSTM-based double shrinkage methods have superior prediction performance compared to LR-based double shrinkage methods. Third, these findings are robust with the use of normalized data, different data frequencies, different carbon trading markets, and different dataset divisions. This study provides new ideas for carbon price prediction, which might have a theoretical and practical contributions to complex and non-linear time series analysis.
Adherence and Effect of Home-Based Rehabilitation with Telemonitoring Support in Patients with Chronic Non-Specific Low Back Pain: A Pilot Study
Authors: Krkoska Peter , Vlazna Daniela , Sladeckova Michaela , Minarikova Jitka , Barusova Tamara , Batalik Ladislav , Dosbaba Filip , Vohanka Stanislav , Adamova Blanka
Published: January 2023

Home-based exercises have been on the rise recently. This pilot study aimed to assess the adherence and effect of a home-based rehabilitation programme using telemonitoring in patients with chronic non-specific low back pain (CNLBP). Twenty-seven patients with CNLBP were enrolled in the study, each of whom underwent a neurological assessment, including patient-oriented measures and a functional assessment—a battery of tests that comprehensively evaluated trunk muscle function. The rehabilitation programme lasted 18 weeks and included daily home-based exercises. A mobile application or an exercise diary was used to monitor compliance. Adherence to the programme was excellent for both the diary and mobile application groups, with 82.3% in the diary group exercising at least once a day and 72.9% twice a day, and 94.8% in the mobile application group exercising at least once a day and 86.6% twice a day. Both patient-oriented and functional outcomes improved significantly; however, the relative changes of the parameters in these two groups did not correlate, which supports the idea that trunk muscle function does not directly relate to patient complaints and that CNLBP is a multifactorial issue. This model of rehabilitation programme should be used in clinical practice, as its adherence and effectiveness seem noticeable.
Intimate Partner Violence and Mental Health: Deepening Our Understanding of Associations, Pathways, and Prevention in Low- and Middle-Income Countries
Authors: Stark Lindsay , Seff Ilana , Mutumba Massy , Fulu Emma
Published: January 2023

Mental health disorders and related symptoms are among the top leading causes of disability adjusted life years (DALYs) among youth (10–24 years) and adults (25–49 years) [...]
How Well Prepared Are Dental Students and New Graduates in Pakistan—A Cross-Sectional National Study
Authors: Javed Muhammad Qasim , Nawabi Shazia , Bhatti Usman Anwer , Atique Sundus , AlAttas Mustafa Hussein , Abulhamael Ayman M. , Zahra Daniel , Ali Kamran
Published: January 2023

The transition of an undergraduate dental student to an actual practicing dentist is a crucial phase and ensuring the preparedness of graduates for the complexity and demands of contemporary dental practice is a challenging task. This study aimed to evaluate the self-perceived preparedness of undergraduate dental students and house officers in the dental colleges of Pakistan. A cross-sectional national study was planned to collect information from dental students and new graduates in Pakistan. The pre-validated Dental Undergraduates Preparedness Assessment Scale (DU-PAS) was used. A purposive sampling technique was utilized to recruit house officers and undergraduate dental students from 27 dental schools in Pakistan. The data analysis was carried out using the R statistical environment for Windows (R Core Team, 2015). A total of 862 responses with 642 females and 219 males were analyzed in the study. Overall, the clinical skills score was 30.56 ± 9.08 and the score for soft skills was 30.54 ± 10.6. The mean age of the participants was 23.42 ± 1.28. Deficiencies were reported in various soft skills and clinical skills attributes. The results highlighted the strengths and weaknesses of dental students and new graduates in Pakistani dental institutions. The findings may be used to further develop and strengthen the teaching and training of dental students in Pakistan.
Effect of Disease Severity, Age of Child, and Clinic No-Shows on Unscheduled Healthcare Use for Childhood Asthma at an Academic Medical Center
Authors: Rangachari Pavani , Parvez Imran , LaFontaine Audrey-Ann , Mejias Christopher , Thawer Fahim , Chen Jie , Pathak Niharika , Mehta Renuka
Published: January 2023

This study examines the influence of various individual demographic and risk factors on the use of unscheduled healthcare (emergency and inpatient visits) among pediatric outpatients with asthma over three retrospective timeframes (12, 18, and 24 months) at an academic health center. Out of a total of 410 children who visited an academic medical center for asthma outpatient care between 2019 and 2020, 105 (26%) were users of unscheduled healthcare for childhood asthma over the prior 12 months, 131 (32%) over the prior 18 months, and 147 (36%) over the prior 24 months. multiple logistic regression (MLR) analysis of the effect of individual risk factors revealed that asthma severity, age of child, and clinic no-shows were statistically significant predictors of unscheduled healthcare use for childhood asthma. Children with higher levels of asthma severity were significantly more likely to use unscheduled healthcare (compared to children with lower levels of asthma severity) across all three timeframes. Likewise, children with three to four clinic no-shows were significantly more likely to use unscheduled healthcare compared to children with zero clinic no-shows in the short term (12 and 18 months). In contrast, older children were significantly less likely to use unscheduled healthcare use compared to younger children in the longer term (24 months). By virtue of its scope and design, this study provides a foundation for addressing a need identified in the literature for short- and long-term strategies for improving supported self-management and reducing unscheduled healthcare use for childhood asthma at the patient, provider, and organizational levels, e.g., (1) implementing telehealth services for asthma outpatient care to reduce clinic no-shows across all levels of asthma severity in the short term; (2) developing a provider–patient partnership to enable patient-centered asthma control among younger children with higher asthma severity in the long term; and (3) identifying hospital–community linkages to address social risk factors influencing clinic no-shows and unscheduled healthcare use among younger children with higher asthma severity in the long term.
Prevalence of Smoking Various Tobacco Types in the Kazakhstani Adult Population in 2021: A Cross-Sectional Study
Authors: Glushkova Natalya , Smailova Dariga , Namazbayeva Zhanar , Mukasheva Gulmira , Zhamakurova Ayaulym , Kuanyshkalieva Asylzhan , Karibayeva Indira K. , Kauysheva Almagul , Otyzbayeva Nurzhamal , Kulzhanov Maksut , Semenova Yuliya
Published: January 2023

Tobacco use was the second-leading risk factor for death, accounting for 15.4% of total deaths in 2019. In 2019, 20.4% (2.7 million) of the adult population in Kazakhstan, 36.5% of men, and 6.0% of women smoked tobacco. A cross-sectional study of a random sample (n = 1201) was conducted between October and December 2021 in accordance with the STEPwise approach. The tobacco-use questions were focused on current and previous smoking status, initiation and duration of smoking, amount of tobacco use, exposure to secondhand smoke, and information related to quitting smoking. From 20.8% of smokers, 93.8% of men and 80.2% of women use tobacco products daily, χ2 = 10.983, p-score < 0.001. The earliest initiation of smoking was 6 years old. The prevalence of smoking tobacco products in Kazakhstan is 20.8%, which means that every fifth adult smokes. In addition, the proportion of smokers among men was 38.5%, and among women, it was 10.1%. A total of 93.8% of men and 80.2% of women smoked daily. The role of healthcare professionals in smoking prevention is very low, and only 16.9% of respondents have been advised to quit smoking in the last 12 months. New interventions for tobacco smoking prevention are urgently needed in Kazakhstan.
Correction: Lander et al. Health Literacy Development among People with Chronic Diseases: Advancing the State of the Art and Learning from International Practices. Int. J. Environ. Res. Public Health 2022, 19, 7315
Authors: Lander Jonas , Dierks Marie-Luise , Hawkins Melanie
Published: January 2023

The authors would like to make corrections to a recently published paper [...]
Onboarding in Polish Enterprises in the Perspective of HR Specialists
Authors: Krugiełka Agnieszka , Bartkowiak Grażyna , Knap-Stefaniuk Agnieszka , Sowa-Behtane Ewa , Dachowski Ryszard
Published: January 2023

The article discusses onboarding in Polish enterprises analyzed from the perspective of HR specialists. The subject of consideration in the article falls within the area of broadly understood concern for the sustainable development and well-being of employees adjusting to a new work environment during their adaptation period. Actions taken by HR specialists have a significant impact on the behavior of employees and their well-being and satisfaction in the new workplace, including commitment and care for all stakeholders involved in building a positive psychological climate at the time of starting work. The aim of the article was to identify onboarding practices implemented in selected types of Polish enterprises and selected factors determining the choice of specific practices, such as analyzing the dependencies between the type of an enterprise and the implementation of onboarding practices in it, identifying the perception of these practices by HR male and female specialists at a given age and with given seniority, and determining whether the practice of assigning a buddy to new employees depends on the type of enterprise. The study described in the article was conducted in 178 medium-sized Polish enterprises, of which, 25 were manufacturing companies, 34 were trading companies, and 119 were service companies. In each enterprise, an HR specialist completed a questionnaire on onboarding. The analysis of the dependencies between the type of company and the type of onboarding implemented in it (general, position, or team onboarding) revealed no statistically significant differences between the types of companies participating in the study and the type of onboarding implemented in them. The discrepancies between preferences for particular onboarding practices and the gender, age, and seniority of HR specialists participating in the study turned out to be statistically significant and indicate that manufacturing companies more often assign a buddy to new employees in their onboarding process than trading and service companies. The conclusions obtained from the research, apart from the cognitive value, have an application value, e.g., regarding the recruitment of HR specialists.
The Experiences of Outreach Support Staff Working with People with Mild Intellectual Disabilities during Different Stages of the COVID-19 Pandemic in the Netherlands: A Qualitative Study
Authors: Vromans Laura , den Boer Maria C. , Frielink Noud , Embregts Petri J. C. M.
Published: January 2023

The COVID-19 pandemic profoundly impacted the work of professionals who support people with intellectual disabilities. This study aimed to explore the experiences of outreach support staff supporting people with mild intellectual disabilities in the Netherlands during different phases of the pandemic between March 2020 and May 2021. Overall, seven outreach support staff from three intellectual disability services participated in this qualitative study. Using semi-structured interviews, participants were interviewed on three occasions between December 2020 and May 2021. A thematic analytical framework was used to analyze the interviews. Four overarching themes could be distinguished based on the data: (1) balancing between one’s professional and personal life; (2) vaccination as both a stress reducer and a source of agitation; (3) service users: vulnerable versus resilient; and (4) contact with colleagues and service users. These themes provided valuable insights into the experiences of outreach support staff during different phases of the pandemic, both in the enduring impact of the pandemic and its measures on support staff, as well as in terms of how the pandemic and its preventive measures impacted their profession.
Developing a Climate Change Risk Perception Model in the Philippines and Fiji: Posttraumatic Growth Plays Central Role
Authors: Sattler David N. , Graham James M. , Whippy Albert , Atienza Richard , Johnson James
Published: January 2023

Background: This two-study paper developed a climate change risk perception model that considers the role of posttraumatic growth (i.e., a reappraisal of life priorities and deeper appreciation of life), resource loss, posttraumatic stress, coping, and social support. Method: In Study 1, participants were 332 persons in the Philippines who experienced Super Typhoon Haiyan. In Study 2, participants were 709 persons in Fiji who experienced Cyclone Winston. Climate change can increase the size and destructive potential of cyclones and typhoons as a result of warming ocean temperatures, which provides fuel for these storms. Participants completed measures assessing resource loss, posttraumatic stress, coping, social support, posttraumatic growth, and climate change risk perception. Results: Structural equation modeling was used to develop a climate change risk perception model with data collected in the Philippines and to confirm the model with data collected in Fiji. The model showed that climate change risk perception was influenced by resource loss, posttraumatic stress, coping activation, and posttraumatic growth. The model developed in the Philippines was confirmed with data collected in Fiji. Conclusions: Posttraumatic growth played a central role in climate change risk perception. Public health educational efforts should focus on vividly showing how climate change threatens life priorities and that which gives life meaning and can result in loss, stress, and hardship. Disaster response organizations may also use this approach to promote preparedness for disaster threats.
The Prevalence of Nutritional Anaemia in Brazilian Pregnant Women: A Systematic Review and Meta-Analysis
Authors: Biete Amanda , Gonçalves Vivian S. S. , Franceschini Sylvia C. C. , Nilson Eduardo A. F. , Pizato Nathalia
Published: January 2023

Despite the global tendency of maternal anaemia to decline, the persistence of anaemia in Brazil is an important health problem given its vulnerability to deficiencies and the significant increase in nutritional requirements during pregnancy. The aim of this study was to estimate the prevalence of anaemia in Brazilian pregnant women through a systematic literature review and meta-analysis. The systematic review was carried out according to Systematic Reviews and Meta-Analyses PRISMA checklist recommendations and using the following electronic databases: Medline, Scopus, Embase, Web of Science, Lilacs, Scielo, Google Scholar, and CAPES Catalog of Theses and Dissertations. Studies that presented a prevalence of anaemia data in Brazilian pregnant women, considering all gestational trimesters, were included. The total sample included 12,792 pregnant women covering all gestational trimesters. The pooled prevalence of anaemia in Brazilian pregnant women was 23% (95% CI: 20–27), with the highest prevalence in the Northeast Region at 26% (95% CI 23–29), while the lowest prevalence was observed in the North Region with 17% (95% CI 14–20). Among the subgroups, no statistical difference was observed. The prevalence of anaemia status in Brazil is still classified as a moderate public health problem according to the World Health Organization maternal anaemia classification.
Glutathione S-Transferase P1 Genetic Variant’s Influence on the HbA1c Level in Type Two Diabetic Patients
Authors: Orlewska Katarzyna , Klusek Justyna , Głuszek Stanisław , Klusek Jolanta , Witczak Bartosz , Wawszczak Monika , Madej Łukasz , Marzec Michał Tomasz , Orlewska Ewa
Published: January 2023

GST (glutathione S-transferases) are capable of influencing glucose homeostasis, probably through regulation of the response to oxidant stress. The aim of our study was to investigate the relationship between GSTP1 gene polymorphism and glycated hemoglobin (HbA1c) levels in type two diabetic (T2D) patients. A total of 307 T2D patients were included. Analysis of the GSTP1 gene polymorphism (rs1695) was conducted using the TaqMan qPCR method endpoint genotyping. HbA1c was determined using a COBAS 6000 autoanalyzer. A univariable linear regression and multivariable linear regression model were used to investigate the association between mean HbA1c level and GSTP1 gene polymorphism, age at T2D diagnosis, T2D duration, therapy with insulin, gender, BMI, smoking status. GSTP1 Val/Val genotype, age at T2D diagnosis, T2D duration and therapy with insulin were statistically significant contributors to HbA1c levels (p < 0.05). Multivariable regression analysis revealed that GSTP1 (Val/Val vs. Ile/Ile) was associated with higher HbA1c even after adjustment for variables that showed a statistically significant relationship with HbA1c in univariable analyses (p = 0.024). The results suggest that GSTP polymorphism may be one of the risk factors for higher HbA1c in T2D patients. Our study is limited by the relatively small sample size, cross-sectional design, and lack of inclusion of other oxidative stress-related genetic variants.