bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Due to ABO Incompatibility and Donor-Recipient Weight Mismatch in Living Donor Kidney Transplantation: A National Cohort Study
Authors: Lee Jun Young , Cha Sung Hwan , Kim Sung Hwa , Jeong Kyung Hwan , Chung Ku Yong , Cho Hong Rae , Lee Juhan , Huh Kyu Ha , Yang Jaeseok , Kim Myoung Soo , Kim Deok Gie ,   on behalf of the Korean Organ Transplantation Registry Study Group
Published: December 2021

The effect of donor-recipient weight mismatch is not well established in ABO-incompatible living donor kidney transplantation (LDKT). A total of 2584 LDKT patients in the Korean Organ Transplantation Registry were classified into four groups according to the presence or absence of ABO incompatibility and donor-recipient weight mismatch (donor-to-recipient weight ratio (DRWR) < 0.8). In a multivariable Cox analysis, the combination of ABO incompatibility and DRWR incompatibility (n = 124) was an independent risk factor for graft survival (HR = 2.73, 95% CI = 1.11–6.70) and patient survival (HR = 3.55, 95% CI = 1.39–9.04), whereas neither factor alone was a significant risk factor for either outcome. The combination of ABO incompatibility and DRWR incompatibility was not an independent risk factor for biopsy-proven graft rejection (HR = 1.27, 95% CI = 0.88–1.82); however, it was an independent risk factor for pneumonia (HR = 2.94, 95% CI = 1.64–5.57). The mortality rate due to infection was higher among patients with both ABO incompatibility and DRWR incompatibility than among patients with neither factor or with either factor alone. The combination of ABO incompatibility and DRWR incompatibility was an independent risk factor for graft and patient survival after LDKT, whereas neither factor alone significantly affected graft or patient survival. Thus, donor-recipient weight matching should be cautiously considered in LDKT with ABO incompatibility.
Biliary Atresia: Clinical Phenotypes and Aetiological Heterogeneity
Authors: Davenport Mark , Muntean Ancuta , Hadzic Nedim
Published: December 2021

Biliary atresia (BA) is an obliterative condition of the biliary tract that presents with persistent jaundice and pale stools typically in the first few weeks of life. While this phenotypic signature may be broadly similar by the time of presentation, it is likely that this is only the final common pathway with a number of possible preceding causative factors and disparate pathogenic mechanisms—i.e., aetiological heterogeneity. Certainly, there are distinguishable variants which suggest a higher degree of aetiological homogeneity such as the syndromic variants of biliary atresia splenic malformation or cat-eye syndrome, which implicate an early developmental mechanism. In others, the presence of synchronous viral infection also make this plausible as an aetiological agent though it is likely that disease onset is from the perinatal period. In the majority of cases, currently termed isolated BA, there are still too few clues as to aetiology or indeed pathogenesis.
Hypophosphatasia
Authors: Tournis Symeon , Yavropoulou Maria , Polyzos Stergios , Doulgeraki Artemis
Published: December 2021

Hypophosphatasia (HPP) is an inherited metabolic disease caused by loss-of-function mutations in the tissue non-specific alkaline phosphatase (TNAP) gene. Reduced activity of TNAP leads to the accumulation of its substrates, mainly inorganic pyrophosphate and pyridoxal-5′-phosphate, metabolic aberrations that largely explain the musculoskeletal and systemic features of the disease. More than 400 ALPL mutations, mostly missense, are reported to date, transmitted by either autosomal dominant or recessive mode. Severe disease is rare, with incidence ranging from 1:100,000 to 1:300,000 live births, while the estimated prevalence of the less severe adult form is estimated to be between 1:3100 to 1:508, in different countries in Europe. Presentation largely varies, ranging from death in utero to asymptomatic adults. In infants and children, clinical features include skeletal, respiratory and neurologic complications, while recurrent, poorly healing fractures, muscle weakness and arthropathy are common in adults. Persistently low serum alkaline phosphatase is the cardinal biochemical feature of the disease. Management requires a dedicated multidisciplinary team. In mild cases, treatment is usually symptomatic. Severe cases, with life-threating or debilitating complications, can be successfully treated with enzyme replacement therapy with asfotase alfa.
Developmental Aspects of SARS-CoV-2, Potential Role of Exosomes and Their Impact on the Human Transcriptome
Authors: Dogra Navneet , Ledesma-Feliciano Carmen , Sen Rwik
Published: December 2021

With over 4.8 million deaths within 2 years, time is of the essence in combating COVID-19. The infection now shows devastating impacts on the younger population, who were not previously predicted to be vulnerable, such as in the older population. COVID-19-related complications have been reported in neonates whose mothers were infected with SARS-CoV-2 during pregnancy, and in children who get infected. Hence, a deeper understanding of the pathophysiology of COVID-19 during various developmental stages and placental transmission is essential. Although a connection has not yet been established between exosomal trafficking and the placental transmission of COVID-19, reports indicate that SARS-CoV-2 components may be trafficked between cells through exosomes. As the infection spreads, the transcriptome of cells is drastically perturbed, e.g., through the severe upregulation of several immune-related genes. Consequently, a major outcome of COVID-19 is an elevated immune response and the detection of viral RNA transcripts in host tissue. In this direction, this review focuses on SARS-CoV-2 virology, its in utero transmission from infected pregnant mothers to fetuses, SARS-CoV-2 and exosomal cellular trafficking, transcriptomic impacts, and RNA-mediated therapeutics against COVID-19. Future research will establish stronger connections between the above processes to develop diagnostic and therapeutic solutions towards COVID-19 and similar viral outbreaks.
A Competitive Sprinter’s Resting Blood Lactate Levels Fluctuate with a One-Year Training Cycle: Case Reports
Authors: Kano Ryotaro , Sato Kohei
Published: December 2021

It has been reported that the variability of resting blood lactate concentration (BLa) is related to metabolic capacity. However, it is unclear whether the resting BLa of athletes can be utilized as a metabolic biomarker. This longitudinal case study tested the hypothesis that resting BLa levels in the morning fluctuate with a 1-year training cycle. The subject was an adult male sprinter, and BLa and blood glucose at the time of waking were measured every day for 1 year. The training cycles were divided into five phases: 1. Basic training: high-intensity and high-volume load; 2. Condition and speed training: high-intensity and low-volume load; 3. Competition training I: track race and high-intensity load; 4. Conditioning for injury; 5. Competition training II. The mean BLa levels in the basic training (1.10 ± 0.32 mmol/L and competition training I (1.06 ± 0.28 mmol/L) phases were significantly lower than in the condition and speed training (1.26 ± 0.40 mmol/L) and conditioning injury (1.37 ± 0.34 mmol/L) phases. The clarified training cycle dependence of resting BLa is suggested to be related to the ability to utilize lactate as an energy substrate with fluctuations in oxidative metabolic capacity. This case report supports the tentative hypothesis that resting BLa may be a biomarker index linked to the metabolic capacity according to the training cycle.
Ultra Low Frequency Wave Produced by Underwater Oscillating Sphere and Its Measurement
Authors: Wang Huan , Fang Erzheng , Wu Mingze , Hong Lianjin , Li Zongru
Published: December 2021

When an underwater target moves in viscous fluid, it may cause the periodic movement of the surrounding fluid and generate ultra-low-frequency (ULF) gravity waves. The initial domain of the gravitational surface wave propagating above the moving target is named circular wave. This article studies the ULF circular wave generated by underwater oscillating sphere, which will provide basis for underwater long-range target detection. Firstly, the circular wave caused by the sphere oscillation in a finite deep fluid is studied based on the theory of linear potential flow. Meanwhile, the multipole expansion theory is established to solve the circular wave field. Secondly, the interface wave generated by the target oscillation in a two-layer fluid are numerically analyzed by comparison with the free surface fluctuation of a single-layer fluid. The results show that the amplitude of the internal interface displacement (AIID) is smaller than that of the free surface (AFSD). When the sphere is in the lower layer, the layering effect of the fluid has significant influences on the AFSD. Finally, the results of the pool experiment verified that the wave generated by the oscillating sphere is the surface gravity wave. Furthermore, the change trend of the test result is consistent with the simulation result.
Development of Novel Forms of Fungal Art Using Aspergillus nidulans
Authors: Grunwald Ofer , Harish Ety , Osherov Nir
Published: December 2021

Fungi are embedded in human culture, tradition, and art, and have featured as inspirational and visual motifs. Psychedelic and medicinal mushrooms have been sculpted, painted, and ingested by our ancestors since prehistory. In modern times, the growing divide between the arts and sciences has delegated fungal art to a niche activity, with the bulk of the focus being on mycelium as a biomaterial. A collaboration between a multidisciplinary artist and a research laboratory, specializing in the molecular study of Aspergillus molds, has allowed us to develop new forms of mycelial art. We describe in detail the development of fungal art techniques using nutrient-rich agar containing Aspergillus nidulans conidia spotted on glass acrylic surfaces or impregnated onto etched acrylic blocks. This approach generates visually and temporally dynamic artwork that is user-friendly, safe, relatively resistant to contamination and easily scalable. Moreover, it offers countless avenues of artistic development based on the diversity of colors, textures and shapes afforded by different fungal species.
The Impact of Endocrine-Disrupting Chemicals in Male Fertility: Focus on the Action of Obesogens
Authors: Rato Luís , Sousa Ana C. A.
Published: December 2021

The current scenario of male infertility is not yet fully elucidated; however, there is increasing evidence that it is associated with the widespread exposure to endocrine-disrupting chemicals (EDCs), and in particular to obesogens. These compounds interfere with hormones involved in the regulation of metabolism and are associated with weight gain, being also able to change the functioning of the male reproductive axis and, consequently, the testicular physiology and metabolism that are pivotal for spermatogenesis. The disruption of these tightly regulated metabolic pathways leads to adverse reproductive outcomes. The permanent exposure to obesogens has raised serious health concerns. Evidence suggests that obesogens are one of the leading causes of the marked decline of male fertility and key players in shaping the future health outcomes not only for those who are directly exposed but also for upcoming generations. In addition to the changes that lead to inefficient functioning of the male gametes, obesogens induce alterations that are “imprinted” on the genes of the male gametes, establishing a link between generations and contributing to the transmission of defects. Unveiling the molecular mechanisms by which obesogens induce toxicity that may end-up in epigenetic modifications is imperative. This review describes and discusses the suggested molecular targets and potential mechanisms for obesogenic–disrupting chemicals and the subsequent effects on male reproductive health.
Dismal Survival in COVID-19 Patients Requiring ECMO as Rescue Therapy after Corticosteroid Failure
Authors: Voicu Sebastian , Goury Antoine , Lacoste-Palasset Thomas , Malissin Isabelle , Fanet Lucie , Souissi Samar , Busto Julia , Legros Vincent , Sutterlin Laetitia , Naim Giulia , M’Rad Aymen , Pepin-Lehaleur Adrien , Deye Nicolas , Mourvillier Bruno , Mégarbane Bruno
Published: December 2021

(1) Background: COVID-19 may lead to refractory hypoxemia requiring venovenous extracorporeal membrane oxygenation (ECMO). Survival rate if ECMO is implemented as rescue therapy after corticosteroid failure is unknown. We aimed to investigate if ECMO implemented after failure of the full-recommended 10-day corticosteroid course can improve outcome. (2) Methods: We conducted a three-center cohort study including consecutive dexamethasone-treated COVID-19 patients requiring ECMO between 03/2020 and 05/2021. We compared survival at hospital discharge between patients implemented after (ECMO-after group) and before the end of the 10-day dexamethasone course (ECMO-before group). (3) Results: Forty patients (28M/12F; age, 57 years (51–62) (median (25th–75th percentiles)) were included, 28 (70%) in the ECMO-before and 12 (30%) in the ECMO-after group. In the ECMO-before group, 9/28 patients (32%) received the 6 mg/day dexamethasone regimen versus 12/12 (100%) in the ECMO-after group (p < 0.0001). The rest of the patients received an alternative dexamethasone regimen consisting of 20 mg/day during 5 days followed by 10 mg/day during 5 days. Patients in the ECMO-before group tended to be younger (57 years (51–59) versus 62 years (57–67), p = 0.053). In the ECMO-after group, no patient (0%) survived while 12 patients (43%) survived in the ECMO-before group (p = 0.007). (4) Conclusions: Survival is poor in COVID-19 patients requiring ECMO implemented after the full-recommended 10-day dexamethasone course. Since these patients may have developed a particularly severe presentation, new therapeutic strategies are urgently required.
The Cardio-Hepatic Relation in STEMI
Authors: Bannon Lian , Merdler Ilan , Bar Nir , Lupu Lior , Banai Shmuel , Jacob Giris , Shacham Yacov
Published: December 2021

Background: Hepatic injury secondary to congestive heart failure is well described, however, only limited data exist about the possible impact of acute cardiac dysfunction on the liver. We aimed to explore the possible cardio-hepatic interaction in patients with myocardial infarction. Material and methods: A single-center retrospective cohort study of 1339 ST elevation myocardial infarction (STEMI) patients who underwent primary coronary intervention between June 2012 to June 2019. Echocardiographic examinations were performed to assess left ventricular ejection fraction (LVEF) and central venous pressure (CVP). Patients were stratified into four groups by their LVEF and CVP levels: LVEF ≥ 45%, and CVP ≤ 10 mm/Hg (n = 853), LVEF < 45% with CVP ≤ 10 mm/Hg (n = 364), EF ≥ 45%, with CVP > 10 mm/Hg (n = 61), and LVEF < 45% with CVP > 10 mm/Hg (n = 61). Patients were evaluated for baseline and peak liver enzymes including alanine transaminase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), and bilirubin. Results: Greater severity of cardiac dysfunction was associated with worse elevation of liver enzymes. We found a graded increase in mean levels of maximal ALT, first and maximal ALP, and first and maximal GGT values. Using propensity score matching to estimate the impact of cardiac dysfunction on liver injury, we chose patients with the worst cardiac function parameters: (LVEF < 45% and CVP >10 mm/Hg; n = 61) and compared them to matched patients with better cardiac function (n = 45). We found a significantly higher level of maximal ALT, first and maximal ALP, and GGT values in the group with the worst cardiac function parameters (p < 0.05). Conclusions: Among patients with STEMI, the combination of decreased LVEF and venous congestion was associated with liver enzymes elevation suggesting a possible cardio-hepatic syndrome.
Analgesia Effect of Verum and Sham Acupuncture Treatments in Primary Dysmenorrhea: A MRI Pilot Study
Authors: Peng Shin-Lei , Yang Hui-Chieh , Lee Yu-Chen , Chen Chun-Ming , Chen Ying-Yu , Tu Cheng-Hao
Published: December 2021

Acupuncture is an alternative treatment for primary dysmenorrhea (PDM). However, mechanisms by which acupuncture exerts its analgesic properties are still unclear. This study aimed to explore the cerebral blood flow (CBF) response to verum and sham acupuncture treatments, and further investigate whether pre-treatment CBF is capable of assessing symptom changes after interventions. A total of 11 PDM patients in the verum group and 12 patients in the sham group participated in this study. Pain rating index (PRI), CBF, and gonadal hormone levels were acquired before and after 8-week treatments. Both verum and sham acupuncture treatments exert its analgesic effect on PDM after intervention as PRI reduced (p < 0.05). Blood gonadal levels were not significantly different after acupuncture in both groups (all p > 0.05). In the verum group, intervention-related decreases in CBF were observed in the right dorsal anterior cingulate cortex. In the sham group, regions identified as showing reductions in CBF after acupuncture included the left ventromedial prefrontal cortex, left caudate, and left insula. Patients with higher baseline CBF in the left precuneus and right hippocampus were accompanied with worse treatment response to acupuncture intervention. Mechanisms of verum and sham acupuncture treatments are dissimilar as manifested by different brain responses.
Prognostic Implications of Right Ventricular Function and Pulmonary Pressures Assessed by Echocardiography in Hospitalized Patients with COVID-19
Authors: Polito Maria Vincenza , Silverio Angelo , Di Maio Marco , Bellino Michele , Scudiero Fernando , Russo Vincenzo , Rasile Barbara , Alfano Carmine , Citro Rodolfo , Parodi Guido , Vecchione Carmine , Galasso Gennaro
Published: December 2021

Aims: Pulmonary involvement in Coronavirus disease 2019 (COVID-19) may affect right ventricular (RV) function and pulmonary pressures. The prognostic value of tricuspid annular plane systolic excursion (TAPSE), systolic pulmonary artery pressure (PAPS), and TAPSE/PAPS ratios have been poorly investigated in this clinical setting. Methods and results: This is a multicenter Italian study, including consecutive patients hospitalized for COVID-19. In-hospital mortality and pulmonary embolism (PE) were identified as the primary and secondary outcome measures, respectively. The study included 227 (16.1%) subjects (mean age 68 ± 13 years); intensive care unit (ICU) admission was reported in 32.2%. At competing risk analysis, after stratifying the population into tertiles, according to TAPSE, PAPS, and TAPSE/PAPS ratio values, patients in the lower TAPSE and TAPSE/PAPS tertiles, as well as those in the higher PAPS tertiles, showed a significantly higher incidence of death vs. the probability to be discharged during the hospitalization. At univariable logistic regression analysis, TAPSE, PAPS, and TAPSE/PAPS were significantly associated with a higher risk of death and PE, both in patients who were and were not admitted to ICU. At adjusted multivariable regression analysis, TAPSE, PAPS, and TAPSE/PAPS resulted in independently associated risk of in-hospital death (TAPSE: OR 0.85, CI 0.74–0.97; PAPS: OR 1.08, CI 1.03–1.13; TAPSE/PAPS: OR 0.02, CI 0.02 × 10−1–0.2) and PE (TAPSE: OR 0.7, CI 0.6–0.82; PAPS: OR 1.1, CI 1.05–1.14; TAPSE/PAPS: OR 0.02 × 10−1, CI 0.01 × 10−2–0.04). Conclusions: Echocardiographic evidence of RV systolic dysfunction, increased PAPS, and poor RV-arterial coupling may help to identify COVID-19 patients at higher risk of mortality and PE during hospitalization.
Correlation between Gut Microbiota and Six Facets of Neuroticism in Korean Adults
Authors: Park Eunkyo , Yun Kyung Eun , Kim Mi-Hyun , Kim Jimin , Chang Yoosoo , Ryu Seungho , Kim Hyung-Lae , Kim Han-Na , Jung Sung-Chul
Published: December 2021

A person high in neuroticism is more likely to experience anxiety, stress, worry, fear, anger, and depression. Previous studies have shown that the gut microbiota can influence personality and mental disorders, including stress, anxiety, and depression, through the gut–brain axis. Here, we investigated the correlations between the sub-facet of neuroticism and gut microbiota using the Revised NEO Personality Inventory and the 16S rRNA gene sequencing data 784 adults. We found that the high anxiety and vulnerability group showed significantly lower richness in microbial diversity than a group with low anxiety and vulnerability. In beta diversity, there was a significant difference between the low and high groups of anxiety, self-consciousness, impulsiveness, and vulnerability. In taxonomic compositions, Haemophilus belonging to Gammaproteobacteria was correlated with the Neuroticism domain as well as N1 anxiety and N6 vulnerability facets. The high N1 anxiety and N6 vulnerability group was correlated with a low abundance of Christensenellaceae belonging to Firmicutes Clostridia. High N4 self-consciousness was correlated with a low abundance of Alistipes and Sudoligranulum. N5 impulsiveness was correlated with a low abundance of Oscillospirales. Our findings will contribute to uncovering the potential link between the gut microbiota and neuroticism, and the elucidation of the correlations of the microbiome–gut–brain axis with behavioral changes and psychiatric cases in the general population.
Detection of Bone Metastases on Bone Scans through Image Classification with Contrastive Learning
Authors: Hsieh Te-Chun , Liao Chiung-Wei , Lai Yung-Chi , Law Kin-Man , Chan Pak-Ki , Kao Chia-Hung
Published: December 2021

Patients with bone metastases have poor prognoses. A bone scan is a commonly applied diagnostic tool for this condition. However, its accuracy is limited by the nonspecific character of radiopharmaceutical accumulation, which indicates all-cause bone remodeling. The current study evaluated deep learning techniques to improve the efficacy of bone metastasis detection on bone scans, retrospectively examining 19,041 patients aged 22 to 92 years who underwent bone scans between May 2011 and December 2019. We developed several functional imaging binary classification deep learning algorithms suitable for bone scans. The presence or absence of bone metastases as a reference standard was determined through a review of image reports by nuclear medicine physicians. Classification was conducted with convolutional neural network-based (CNN-based), residual neural network (ResNet), and densely connected convolutional networks (DenseNet) models, with and without contrastive learning. Each set of bone scans contained anterior and posterior images with resolutions of 1024 × 256 pixels. A total of 37,427 image sets were analyzed. The overall performance of all models improved with contrastive learning. The accuracy, precision, recall, F1 score, area under the receiver operating characteristic curve, and negative predictive value (NPV) for the optimal model were 0.961, 0.878, 0.599, 0.712, 0.92 and 0.965, respectively. In particular, the high NPV may help physicians safely exclude bone metastases, decreasing physician workload, and improving patient care.
The SGLT-2 Inhibitors in Personalized Therapy of Diabetes Mellitus Patients
Authors: Tilinca Mariana , Tiuca Robert , Tilea Ioan , Varga Andreea
Published: December 2021

Diabetes mellitus (DM) represents a major public health problem, with yearly increasing prevalence. DM is considered a progressive vascular disease that develops macro and microvascular complications, with a great impact on the quality of life of diabetic patients. Over time, DM has become one of the most studied diseases; indeed, finding new pharmacological ways to control it is the main purpose of the research involved in this issue. Sodium–glucose cotransporter 2 inhibitors (SGLT-2i) are a modern drug class of glucose-lowering agents, whose use in DM patients has increased in the past few years. Besides the positive outcomes regarding glycemic control and cardiovascular protection in DM patients, SGLT-2i have also been associated with metabolic benefits, blood pressure reduction, and improved kidney function. The recent perception and understanding of SGLT-2i pathophysiological pathways place this class of drugs towards a particularized patient-centered approach, moving away from the well-known glycemic control strategy. SGLT-2i have been shown not only to reduce death from cardiovascular causes, but also to reduce the risk of stroke and heart failure hospitalization. This article aims to review and highlight the existing literature on the effects of SGLT-2i, emphasizing their role as oral antihyperglycemic agents in type 2 DM, with important cardiovascular and metabolic benefits.
Evidence for the Association between the Intronic Haplotypes of Ionotropic Glutamate Receptors and First-Episode Schizophrenia
Authors: Hirschfeldova Katerina , Cerny Jiri , Bozikova Paulina , Kuchtiak Viktor , Rausch Tobias , Benes Vladimir , Spaniel Filip , Gregus David , Horacek Jiri , Vyklicky Ladislav , Balik Ales
Published: December 2021

The heritable component of schizophrenia (SCH) as a polygenic trait is represented by numerous variants from a heterogeneous group of genes each contributing a relatively small effect. Various SNPs have already been found and analyzed in genes encoding the NMDAR subunits. However, less is known about genetic variations of genes encoding the AMPA and kainate receptor subunits. We analyzed sixteen iGluR genes in full length to determine the sequence variability of iGluR genes. Our aim was to describe the rate of genetic variability, its distribution, and the co-occurrence of variants and to identify new candidate risk variants or haplotypes. The cumulative effect of genetic risk was then estimated using a simple scoring model. GRIN2A-B, GRIN3A-B, and GRIK4 genes showed significantly increased genetic variation in SCH patients. The fixation index statistic revealed eight intronic haplotypes and an additional four intronic SNPs within the sequences of iGluR genes associated with SCH (p < 0.05). The haplotypes were used in the proposed simple scoring model and moreover as a test for genetic predisposition to schizophrenia. The positive likelihood ratio for the scoring model test reached 7.11. We also observed 41 protein-altering variants (38 missense variants, four frameshifts, and one nonsense variant) that were not significantly associated with SCH. Our data suggest that some intronic regulatory regions of iGluR genes and their common variability are among the components from which the genetic predisposition to SCH is composed.
Identification of a Rare Novel KMT2C Mutation That Presents with Schizophrenia in a Multiplex Family
Authors: Chen Chia-Hsiang , Huang Ailing , Huang Yu-Shu , Fang Ting-Hsuan
Published: December 2021

Schizophrenia is a complex genetic disorder involving many common variants with modest effects and rare mutations with high penetrance. Rare mutations associated with schizophrenia are highly heterogeneous and private for affected individuals and families. Identifying such mutations can help establish the molecular diagnosis, elucidate the pathogenesis, and provide helpful genetic counseling for affected patients and families. We performed a whole-exome sequencing analysis to search for rare pathogenic mutations co-segregating with schizophrenia transmitted in a dominant inheritance in a two-generation multiplex family. We identified a rare missense mutation H1574R (Histidine1574Arginine, rs199796552) of KMT2C (lysine methyltransferase 2C) co-segregating with affected members in this family. The mutation is a novel deleterious mutation of KMT2C, not reported before in the literature. The KMT2C encodes a histone 3 lysine 4 (H3K4)-specific methyltransferase and involves epigenetic regulation of brain gene expression. Mutations of KMT2C have been found in neurodevelopmental disorders, such as Kleefstra syndrome, intellectual disability, and autism spectrum disorders. Our finding suggests that schizophrenia might be one of the clinical phenotype spectra of KMT2C mutations, and KMT2C might be a novel risk gene for schizophrenia. Nevertheless, the co-segregation of this mutation with schizophrenia in this family might also be due to chance; functional assays of this mutation are needed to address this issue.
Concurrent Cholecystectomy Is Associated with a Lower Risk of Recurrence after Curative Resection in Early-Stage Hepatocellular Carcinoma: A 10 Year Observational Single-Center Study
Authors: Chen Yu-Syuan , Yang Shih-Yu , Wang Pei-Ming , Wang Chih-Chi , Yong Chee-Chien , Chen Ding-Wei , Liu Yueh-Wei , Chuang Ching-Hui , Huang Pao-Yuan , Yao Chih-Chien , Lin Yen-Po , Tsai Ming-Chao
Published: December 2021

Background: Cholecystectomy has been reported to be associated with increased risk of developing hepatocellular carcinoma (HCC). However, there is little information about the impact of cholecystectomy on the outcome of HCC. Aims: To evaluate the long-term effect of concurrent cholecystectomy on recurrence and overall survival in HCC after curative hepatectomy. Patients and Methods: We retrospectively enrolled 857 patients with BCLC stage 0 or A HCC who underwent primary resection from January 2001 to June 2016. The impact of concurrent cholecystectomy on overall survival (OS) and recurrence-free survival (RFS) were analyzed by Cox’s proportional hazards models after one-to-one propensity score matching (PSM). Results: Of the 857 patients, 539 (62.9%) received concurrent cholecystectomy (cholecystectomy group) and 318 (37.1%) did not (non-cholecystectomy group). During the mean follow-up period of 75.0 months, 471 (55.0%) patients experienced recurrence, and 321 (37.5%) died. RFS and OS were not significantly different between the groups. After PSM, a total of 298 patients were enrolled in each group. RFS was significantly higher in the cholecystectomy than non-cholecystectomy group (p = 0.044). In multivariate analysis, age (p = 0.022), serum AFP (p = 0.008), liver cirrhosis (p < 0.001), diabetes (p = 0.004), tumor number (p = 0.005), tumor size (p = 0.002), histological grade (p = 0.001), microvascular invasion (p < 0.001) and cholecystectomy (p = 0.021) were independent risk factors for HCC recurrence. However, there were no significant differences in OS between the cholecystectomy and non-cholecystectomy groups. Conclusions: Concurrent cholecystectomy may reduce recurrence in early-stage HCC after curative resection. Further studies are needed to validate our results.
The Utility of Pharmacogenetics Testing in Psychiatric Populations
Authors: Jürgens Gesche
Published: December 2021

The implementation of pharmacogenetic tests including multiple gene variants has shown promising potential as a decision-making tool for optimizing psychopharmacological treatment regimens and reducing treatment costs. However, the varying clinical validity of gene variants included in pharmacogenetic test batteries, and inconsistencies in their translation into medical recommendations between commercially available pharmacogenetic tests, complicates their rational implementation. Thus, there is a need for well-designed, reproducible studies documenting the clinical significance of the various genetic variants.
Energy-Aware Wireless Sensor Networks for Smart Buildings: A Review
Authors: Naji Najem , Abid Mohamed Riduan , Krami Nissrine , Benhaddou Driss
Published: December 2021

The design of Wireless Sensor Networks (WSN) requires the fulfillment of several design requirements. The most important one is optimizing the battery’s lifetime, which is tightly coupled to the sensor lifetime. End-users usually avoid replacing sensors’ batteries, especially in massive deployment scenarios like smart agriculture and smart buildings. To optimize battery lifetime, wireless sensor designers need to delineate and optimize active components at different levels of the sensor’s layered architecture, mainly, (1) the number of data sets being generated and processed at the application layer, (2) the size and the architecture of the operating systems (OS), (3) the networking layers’ protocols, and (4) the architecture of electronic components and duty cycling techniques. This paper reviews the different relevant technologies and investigates how they optimize energy consumption at each layer of the sensor’s architecture, e.g., hardware, operating system, application, and networking layer. This paper aims to make the researcher aware of the various optimization opportunities when designing WSN nodes. To our knowledge, there is no other work in the literature that reviews energy optimization of WSN in the context of Smart Energy-Efficient Buildings (SEEB) and from the formerly four listed perspectives to help in the design and implementation of optimal WSN for SEEB.
Vascular Access Flow during Dialysis: Does Needle Orientation Matter?
Authors: Tsangalis Georgios , Loizon Valérie
Published: December 2021

Background: Monitoring of vascular access outflow (VAO) in dialysis is based on the indicator dilution method by ultrasound (UD). The role of arterial needle orientation in VAO measurement is not clear. We compared the impact of the retrograde (RET) versus the antegrade orientation (ANT) in terms of (a) VAO (UD) and (b) dialysis adequacy. Moreover, we compared VAO (UD ANT and RET orientation) with VAO measured by Doppler ultrasound. Methods: 22 patients participated in the study. Inclusion criteria: Dialysis > 6 months with a functioning AVF, no stenosis, no active infection, EF > 45% and informed consent. 4 flow measurements were taken on the same dialysis day (4 consecutive weeks). To account for blood pressure variation, we “corrected” VAO for a mean arterial pressure of 100 mmHg. Doppler VAO was measured just before dialysis. Means were compared by the paired t-test. For correlation and agreement, linear regression and Bland-Altman analysis were performed respectively. Results: Mean VAO (UD) was higher in the (ANT) versus the (RET) orientation: 1286.17 mL/min (SD = 455.78, 95%CI = 1084–1488) versus 1189.96 mL/min (SD = 401.05, 95%CI = 1012–1368) (p = 0.013) with a mean difference of 96.21 mL/min (5.66%). Mean Kt/V (RET orientation) was 1.57 (SD = 0.10, 95%CI = 1.52–1.61) versus 1,55 (SD = 0.10, 95%CI = 1.50–1.60) (ANT) orientation (p = 0.062). Recirculation was always 0%. The mean VAO (Doppler) was 1079.54 mL/min (SD = 356.04, 95%CI = 922–1237), 16% lower than VAO measured by UD with (ANT) orientation (p = 0.009) and 9.3% lower than the VAO in the (RET) orientation (p = 0.113). Linear regression analysis showed that VA flows (ANT versus RET) orientation of the needle correlates well between them (r = 0.93, p < 0.001) but show poor agreement (Bland–Altman analysis). Conclusion: VAO (UD) in the RET orientation was significantly lower than VAO in the ANT orientation and more consistent with VAO assessed by Doppler without influencing dialysis adequacy. Therefore, when using UD for VAO surveillance, the RET orientation should be used.
Deriving the Main Cultivation Direction from Open Remote Sensing Data to Determine the Support Practice Measure Contouring
Authors: Scholand Dominik , Schmalz Britta
Published: December 2021

The P-factor for support practice of the Universal Soil Loss Equation (USLE) accounts for soil conservation measures and leads to a significant reduction in the modelled soil loss. However, in the practical application, the P-factor is the most neglected factor overall due to high effort for determining or lack of input data. This study provides a new method for automatic derivation of the main cultivation direction from seed rows and tramlines on agricultural land parcels using the Fast Line Detector (FLD) of the Open Computer Vision (OpenCV) package and open remote sensing data from Google Earth™. Comparison of the cultivation direction with the mean aspect for each land parcel allows the determination of a site-specific P-factor for the soil conservation measure contouring. After calibration of the FLD parameters, the success rate in a first application in the low mountain range Fischbach catchment, Germany, was 77.7% for 278 agricultural land parcels. The main reasons for unsuccessful detection were problems with headland detection, existing soil erosion, and widely varying albedo within the plots as well as individual outliers. The use of a corrected mask and enhanced parameterization offers promising improvements for a higher success rate of the FLD.
Rooting Volume Impacts Growth, Coverage and Thermal Tolerance of Green Façade Climbing Plants
Authors: Chung Pei-Wen , Livesley Stephen J. , Rayner John P. , Farrell Claire
Published: December 2021

Green façades can provide cooling benefits through the shading of walls, evapotranspiration, and insulation. These benefits depend on good plant coverage and tolerance of heat stress. This requires sufficient rooting volume for plant growth and an adequate supply of moisture. On high-rise buildings, plants can be constrained by small rooting volumes due to engineering weight limits and cost. We assessed effects of rooting volume (21, 42, and 63 L) on the growth and coverage of Akebia quinata and Pandorea pandorana and leaf stress (chlorophyll fluorescence) in response to increasing air temperatures. We showed that 42 and 63 L rooting volumes significantly increased early plant growth and the percentage wall coverage for both species. Specific leaf area was significantly greater when grown in 63 L compared with 21 L. Shoot/root ratio did not change with rooting volumes. Regardless of rooting volume, higher air temperatures on west-facing aspects led to afternoon leaf stress. In practice, for each cubic meter of rooting volume, 21 m2 (P. pandorana) and 10 m2 (A. quinata) canopy coverage can be expected within six months.
Research on the Evaluation of Real Estate Inventory Management in China
Authors: Li Weiwei , Weng Lisheng , Zhao Kaixu , Zhao Sidong , Zhang Ping
Published: December 2021

Inventory management not only determines the health of the real estate market development, but also affects the regional economy and the capacity of sustainable social development. In this paper we use the DPSIR framework to integrate multi-dimensional influence factors, such as economic, social, and environmental factors, to construct a real estate inventory management performance evaluation and obstacle diagnosis model, and conduct an empirical study on 31 Chinese provinces and cities. The results show that: first, China’s real estate inventory is huge in size, with significant spatial heterogeneity and agglomeration; second, China’s real estate inventory management performance is unsatisfactory and still shows no improvement despite the strong policy intervention of the central and local governments; third, the obstacle factors of real estate inventory management are becoming increasingly diversified and complicated, with great differences among provinces—significantly, Profits of Real Estate Enterprises, Disposable Income of Urban Residents, Financial Revenue, Per Capital GDP, Resident Population, Gross Domestic Product, Total Retail Sales of Social Consumer Goods, Financial Expense, and Loans Balance of Financial Institutes are critical obstacle factors; and fourth, it is suggested that, on the basis of mastering the actual conditions of supply and demand in the real estate market, differentiated and precise response strategies should be formulated by integrating near-term and long-term goals, direct and indirect forces, and administrative and market instruments.
Rate of Fen-Peat Soil Subsidence Near Drainage Ditches (Central Poland)
Authors: Oleszczuk Ryszard , Zając Ewelina , Urbański Janusz , Jadczyszyn Jan
Published: December 2021

This study analyzed design depths (to), post-subsidence depths (t), shallowing magnitudes (d = to − t) and ratio values (d/t) of 12 drainage ditches in a fragment of the drained Solec fen-peat (central Poland) over a period of 47 years between 1967 and 2014. A significant decrease of the designed depth of the ditches to was shown, from the average designed value of 0.97 m to their average depth after subsidence, t = 0.71 m. The ratio (d/t) of 0.41, which is associated with the degree of organic matter decomposition, indicated medium degree of peat decomposition. The average values of bank and bottom subsidence of the ditches during the analyzed period, 1967–2014, were 0.43 m and 0.17 m, respectively. The values of the average annual rate of land surface subsidence in the vicinity of the ditches were varied and within the range of 0.09 cm year−1 to 1.70 cm year−1, with an average of 0.92 cm year−1. Two linear empirical equations were proposed to calculate the amount of subsidence and the average annual rate of subsidence of peat soil surface near the drainage ditch route, based on the knowledge of the initial thickness of the peat deposit. The results of calculations using the equations proposed by the authors were compared with calculations of the same parameters using 10 equations published in the literature. The results obtained using the proposed equations were mostly larger than those calculated with literature-published equations.
Evaluating the Dominant Controls of Water Erosion in Three Dry Valley Types Using the RUSLE and Geodetector Method
Authors: Yang Chao , Fan Jianrong , Liu Jiali , Xu Fubao , Zhang Xiyu
Published: December 2021

The dry valley is a unique geographic phenomenon in Southwest China with severe water erosion. However, little is known regarding its dominant controls and the discrepancies between dry valley subtypes, leading to the poor management of water erosion. To solve these problems, the revised universal soil loss equation (RUSLE) and Geodetector method were used in a dry temperate (DT), dry warm (DW), and dry hot (DH) valley. Results indicated that dry valleys suffer severe water erosion with a value of 64.78, 43.85, and 33.81 t·ha−1·yr−1. The Geodetector method is proven to be an efficient tool to quantify the dominant factor of water erosion. It was established that land use types (LUT) have the closest relationship with water erosion. The controls for water erosion could be better explained by multi-factor interactions analysis, particularly for the combination of slope and LUT in DW (q = 0.71) and DH (q = 0.66). Additionally, regions at high risk of water erosion were characterized by steep slope (>30°) and low vegetation coverage (<50%) in DT, while the opposite is shown in DH. These findings could provide insight for guiding soil erosion management and ecological restoration strategies that balance economic and environmental sustainability.
Green Infrastructure, Urbanization, and Ecosystem Services: The Main Challenges for Russia’s Largest Cities
Authors: Klimanova Oxana , Illarionova Olga , Grunewald Karsten , Bukvareva Elena
Published: December 2021

Globally, the process of urbanization is transforming land use and, as a consequence, reducing the efficiency of ecosystem services (ESs), which ensure a healthy and comfortable urban environment. In cities, green infrastructure (GI) is a key source of urban ESs. Russia is a highly urbanized country: 70% of its population live in towns or cities. As cities continue to expand, they are swallowing unsealed lands that support ESs. In this paper, we present the results of an analysis of the current state and recent changes in urban GI in Russia’s 16 largest cities, including a biophysical evaluation of key urban ESs, using remote sensing data and freely available statistics. Our analysis identifies a weak correlation between GI area, ES volume, and favorable climate conditions as well as a stronger correlation between ESs and the increasing rate of urbanization. Considering Russia’s high level of urbanization, the key importance of ESs for the well-being of citizens, and ongoing reductions of urban GI, major revisions to urban planning policy are required. Indicators of urban biodiversity, GI, and ESs should be incorporated into decision-making processes. In particular, it is vital that the accounting and monitoring of GI and ESs are established in all of Russia’s larger cities.
Effects of Meteorological Parameters on Surface Water Loss in Burdur Lake, Turkey over 34 Years Landsat Google Earth Engine Time-Series
Authors: Abujayyab Sohaib K. M. , Almotairi Khaled H. , Alswaitti Mohammed , Amr Salem S. Abu , Alkarkhi Abbas F. M. , Taşoğlu Enes , Hussein Ahmad MohdAziz
Published: December 2021

The current work aims to examine the effect of meteorological parameters as well as the temporal variation on the Burdur Lake surface water body areas in Turkey. The data for the surface area of Burdur Lake was collected over 34 years between 1984 and 2019 by Google Earth Engine. The seasonal variation in the water bodies area was collected using our proposed extraction method and 570 Landsat images. The reduction in the total area of the lake was observed between 206.6 km2 in 1984 to 125.5 km2 in 2019. The vegetation cover and meteorological parameters collected that affect the observed variation of surface water bodies for the same area include precipitation, evapotranspiration, albedo, radiation, and temperature. The selected meteorological variables influence the reduction in lake area directly during various seasons. Correlations showed a strong positive or negative significant relationship between the reduction and the selected meteorological variables. A factor analysis provided three components that explain 82.15% of the total variation in the data. The data provide valuable references for decision makers to develop sustainable agriculture and industrial water use policies to preserve water resources as well as cope with potential climate changes.
Methodological Challenges in Studying Trust in Natural Resources Management
Authors: Sohns Antonia , Hickey Gordon M. , de Vries Jasper R. , Temby Owen
Published: December 2021

Trust has been identified as a central characteristic of successful natural resource management (NRM), particularly in the context of implementing participatory approaches to stakeholder engagement. Trust is, however, a multi-dimensional and multi-level concept that is known to evolve recursively through time, challenging efforts to empirically measure its impact on collaboration in different NRM settings. In this communication we identify some of the challenges associated with conceptualizing and operationalizing trust in NRM field research, and pay particular attention to the inter-relationships between the concepts of trust, perceived risk and control due to their multi-dimensional and interacting roles in inter-organizational collaboration. The challenge of studying trust begins with its conceptualization, which impacts the terminology being used, thereby affecting the subsequent operationalization of trust in survey and interview measures, and the interpretation of these measures by engaged stakeholders. Building from this understanding, we highlight some of the key methodological considerations, including how trust is being conceptualized and how the associated measures are being developed, deployed, and validated in order to facilitate cross-context and cross-level comparisons. Until these key methodological issues are overcome, the nuanced roles of trust in NRM will remain unclear.