bluuebunny/crossref_metadata_2025 huggingface dataset:
Ship Deficiency Data of Port State Control to Identify Hidden Risk of Target Ship
Authors: Shen Jian-Hung , Liu Chung-Ping , Chang Ki-Yin , Chen Yung-Wei
Published: October 2021

In the new inspection regime (NIR) of port state control (PSC), the criteria for being judged as a standard risk ship (SRS) is too broad. Some ships are classified as SRS even though they have a large number of ship deficiencies. This paper develops a selection system to identify the hidden risk of target ships in the SRS category using PSC inspection records. This system allows the target ship to be used to help reduce cases of flags being greylisted or blacklisted, which can cause huge shipping losses. This study analyzes ship deficiency data in the Tokyo memorandum of understanding (Tokyo MoU) database. It adopts the multiple criteria decision making (MCDM) model as a data processing technique to build a risk assessment scale. It uses fuzzy importance performance analysis (F-IPA) and technology for order preference by similarity to the ideal solution (TOPSIS) for its analysis. Subsequently, the weights of F-IPA and TOPSIS are adopted into the MCDM model. This article also consulted the Tokyo MoU database. It has been verified that the next time PSC inspection, the system hits 83.3% of the hidden risk ships in the SRS category. Thus, this system will help inspectors be more insightful for target ships.
A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host
Authors: Cornejo Carolina , Hisano Sakae , Bragança Helena , Suzuki Nobuhiro , Rigling Daniel
Published: October 2021

Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Cocultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae upon virus infection. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.
Jumping the Shark: White Shark Representations in Great White Serial Killer Lives—The Fear and the (Pseudo-)Science
Authors: Cermak Iri
Published: October 2021

Sharks are among the most endangered nonhuman animals on the planet because of industrial fishing, the shark meat and fin trade, expanding recreational fishing, and other anthropogenic causes. White sharks (Carcharodon carcharias), the most visible in popular culture, remain vulnerable (VU, IUCN Red List) and understudied, although population recovery is having a measure of success in regions like the Eastern Pacific and the Northern Atlantic of the United States. As numbers rise, Jaws associations also remain in vogue in programming that emphasizes human–wildlife** conflict such as Shark Week’s Great White Serial Killer Lives. Network marketing typically promotes this content by hyping shark science. Textual analysis, however, suggests that exposure to pseudoscientific narratives and unethical fear-inducing images is counterproductive to wider support for conservation programs and public recognition for sharks’ rights to their habitats.
An Integrated Approach for Evaluating Lean Innovation Practices in the Pharmaceutical Supply Chain
Authors: Meidute-Kavaliauskiene Ieva , Cebeci Halil Ibrahim , Ghorbani Shahryar , Činčikaitė Renata
Published: October 2021

Backgroung: Lean innovation focuses on minimizing waste in the product development stages in order to increase productivity by obtaining customer feedback more quickly and efficiently. The usage of lean innovation practices in product development stages in the pharmaceutical supply chain is the topic of an increasing amount of research on the critical question of how lean innovation practices can be implemented in a pharmaceutical supply chain or logistic sector. To answer this question, we first identified lean innovation practices by reviewing the literature. Methods: the identified practices were screened using the fuzzy Delphi method (FDM). The expert panel included eight persons working in pharmaceutical supply chain fields. In the next step, the causal relationships between practices were analyzed using the Gray DEMATEL (GDEMATEL) technique. Results: show that technological knowledge was the most crucial factor in lean innovation practices in the pharmaceutical supply chain. Conclusions: Actualizing lean innovation in the supply chain is more than just utilizing the correct strategies and instruments. To execute lean innovation effectively, a reevaluation must be accomplished: A culture that recognizes requirements for change and is set up for consistent change is essential. Methodological strategies such as the value system cannot be set up as a one-time strategy. To execute lean innovation on a long-haul premise, members must be included and become acclimated to a proceeding with the progress process. Changes in forms are frequently used because of an absence of association of suppliers, regardless of whether measures are sensible.
The Applicability of Die Cast A356 Alloy to Additive Friction Stir Deposition at Various Feeding Speeds
Authors: Alzahrani Bandar , El-Sayed Seleman Mohamed M. , Ahmed Mohamed M. Z. , Elfishawy Ebtessam , Ahmed Adham M. Z. , Touileb Kamel , Jouini Nabil , Habba Mohamed I. A.
Published: October 2021

In the current investigation, additive friction stir-deposition (AFS-D) of as-cast hypoeutectic A356 Al alloy was conducted. The effect of feeding speeds of 3, 4, and 5 mm/min at a constant rotational speed of 1200 rpm on the macrostructure, microstructure, and hardness of the additive manufacturing parts (AMPs) was investigated. Various techniques (OM, SEM, and XRD) were used to evaluate grain microstructure, presence phases, and intermetallics for the as-cast material and the AMPs. The results showed that the friction stir deposition technique successfully produced sound additive manufactured parts at all the applied feeding speeds. The friction stir deposition process significantly improved the microstructure of the as-cast alloy by eliminating porosity and refining the dendritic α-Al grains, eutectic Si phase, and the primary Si plates in addition to intermetallic fragmentation. The mean values of the grain size of the produced AMPs at the feeding speeds of 3, 4, and 5 mm/min were 0.62 ± 0.1, 1.54 ± 0.2, and 2.40 ± 0.15 µm, respectively, compared to the grain size value of 30.85 ± 2 for the as-cast alloy. The AMPs exhibited higher hardness values than the as-cast A356 alloy. The as-cast A356 alloy showed highly scattered hardness values between 55 and 75.8 VHN. The AMP fabricated at a 3 mm/min feeding speed exhibited the maximum hardness values between 88 and 98.1 VHN.
Explosive Spalling Behavior of Single-Sided Heated Concrete According to Compressive Strength and Heating Rate
Authors: Hwang Euichul , Kim Gyuyong , Choe Gyeongcheol , Yoon Minho , Son Minjae , Suh Dongkyun , Eu Hamin , Nam Jeongsoo
Published: October 2021

In this study, the effects of heating rate and compressive strength on the spalling behavior of single-sided heated ring-restrained concrete with compressive strengths of 60 and 100 MPa were investigated. The vapor pressure and restrained stress inside the concrete were evaluated under fast- and slow-heating conditions. Regardless of the heating rate, the concrete vapor pressure and restrained stress increased as the temperature increased, and it was confirmed that spalling occurred in the 100-MPa concrete. Specifically, it was found that moisture migration and restrained stress inside the concrete varied depending on the heating rate. Under fast heating, moisture clogging and restrained stress occurred across the concrete surface, causing continuous surface spalling for the 100-MPa concrete. Under slow heating, moisture clogging occurred, and restrained stress continuously increased in the deep area of the concrete cross-section owing to the small internal temperature difference, resulting in explosive spalling for the 100-MPa concrete with a dense internal structure. Additionally, while the tensile strength of concrete is reduced by heating, stress in the heated surface direction is generated by restrained stress. The combination of stress in the heated concrete surface and the internal vapor pressure generates spalling. The experimental results confirm that heating rate has a significant influence on moisture migration and restrained stress occurrence inside concrete, which are important factors that determine the type of spalling.
In-Situ Reaction Method to Synthetize Constant Solid-State Composites as Phase Change Materials for Thermal Energy Storage
Authors: Yang Bo , Liu Yang , Ye Wenjie , Wang Qiyang , Yang Xiao , Yang Dongmei
Published: October 2021

The encapsulation and heat conduction of molten salt are very important for its application in heat storage systems. The general practice is to solidify molten salt with ceramic substrate and enhance heat conduction with carbon materials, but the cycle stability is not ideal. For this reason, it is of practical significance to study heat storage materials with a carbon-free thermal conductive adsorption framework. In this paper, the in-situ reaction method was employed to synthetize the constant solid-state composites for high-temperature thermal energy storage. AlN is hydrolyzed and calcined to form h-Al2O3 with a mesoporous structure to prevent the leakage of molten eutectic salt at high temperature. Its excellent thermal conductivity simultaneously improves the thermal conductivity of the composites. It is found that 15CPCMs prepared with 15% water addition have the best thermal conductivity (4.928 W/m·K) and mechanical strength (30.2 MPa). The enthalpy and the thermal storage density of 15CPCMs are 201.4 J/g and 1113.6 J/g, respectively. Due to the excellent leak-proof ability and lack of carbon materials, the 15CPCMs can maintain almost no mass loss after 50 cycles. These results indicate that 15CPCMs have promising prospects in thermal storage applications.
Preparation of TiO2/Black Talc Composite Photocatalyst and the Research on Its Adsorption-Degradation Coupling Effects
Authors: Shuai Huan , Wang Yuxin , Wang Jiao , Du Gaoxiang , Chen Daimei , Liang Yu
Published: October 2021

In this paper, a TiO2/black talc composite photocatalyst was prepared by the sol-gel method using TBOT as titanium source and black talc as carrier. Rhodamine B was used as the targeted pollutant to study the adsorption role of carbon in black talc. The results showed that with the adsorption-degradation cycles, the illumination time can be reduced by 40%. The adsorption rate and degradation rate of the composite photocatalyst was also increased. The degradation rate of Rhodamine B reached more than 95%, which fully shows the synergistic effect between TiO2 nanoparticles and black talc. In this way, the adsorption-degradation coupling of the photocatalyst could be realized.
The Effects of Three Remineralizing Agents on the Microhardness and Chemical Composition of Demineralized Enamel
Authors: Salinovic Ivan , Schauperl Zdravko , Marcius Marijan , Miletic Ivana
Published: October 2021

This study aimed to determine the effects of three different varnish materials (containing casein phosphopeptide-amorphous calcium phosphate, nano-hydroxyapatite, and fluoride) on enamel. Thirty-three extracted human third molars were used for specimen preparation. These were demineralized using phosphoric acid. Three experimental groups (n = 11) were treated with 3M™ Clinpro™ White Varnish, MI Varnish®, and Megasonex® toothpaste, respectively, every twenty-four hours for fourteen days. Analysis of the microhardness of the specimens’ enamel surfaces was carried out via the Vickers method, and by scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). Analysis was performed at three stages: at baseline value, after demineralization, and after the period of remineralization. Data were subjected to Scheffe’s post hoc test. The mean microhardness values (HV0.1) obtained for the group of samples treated with MI Varnish® were higher compared with the other two groups (p = 0.001 for both comparisons), while the first and third groups did not differ significantly from each other (p = 0.97). SEM analysis showed uneven patterns and porosities on all samples tested. EDS results showed an increase in the mineral content of the examined samples, with the highest mineral content observed in the MI Varnish® group. It can be concluded that MI Varnish® use has a better remineralization effect on enamel than the other two materials.
Transient Behavior of the MAP/M/1/N Queuing System
Authors: Vishnevsky Vladimir , Vytovtov Konstantin , Barabanova Elizaveta , Semenova Olga
Published: October 2021

This paper investigates the characteristics of the MAP/M/1/N queuing system in the transient mode. The matrix method for solving the Kolmogorov equations is proposed. This method makes it possible, in general, to obtain the main characteristics of the considered queuing system in a non-stationary mode: the probability of losses, the time of the transient mode, the throughput, and the number of customers in the system at time t. The developed method is illustrated by numerical calculations of the characteristics of the MAP/M/1/3 system in the transient mode.
Attribute Selecting in Tree-Augmented Naive Bayes by Cross Validation Risk Minimization
Authors: Chen Shenglei , Zhang Zhonghui , Liu Linyuan
Published: October 2021

As an important improvement to naive Bayes, Tree-Augmented Naive Bayes (TAN) exhibits excellent classification performance and efficiency since it allows that every attribute depends on at most one other attribute in addition to the class variable. However, its performance might be lowered as some attributes might be redundant. In this paper, we propose an attribute Selective Tree-Augmented Naive Bayes (STAN) algorithm which builds a sequence of approximate models each involving only the top certain attributes and searches the model to minimize the cross validation risk. Five different approaches to ranking the attributes have been explored. As the models can be evaluated simultaneously in one pass learning through the data, it is efficient and can avoid local optima in the model space. The extensive experiments on 70 UCI data sets demonstrated that STAN achieves superior performance while maintaining the efficiency and simplicity.
Memorizing Schröder’s Method as an Efficient Strategy for Estimating Roots of Unknown Multiplicity
Authors: Cordero Alicia , Neta Beny , Torregrosa Juan R.
Published: October 2021

In this paper, we propose, to the best of our knowledge, the first iterative scheme with memory for finding roots whose multiplicity is unknown existing in the literature. It improves the efficiency of a similar procedure without memory due to Schröder and can be considered as a seed to generate higher order methods with similar characteristics. Once its order of convergence is studied, its stability is analyzed showing its good properties, and it is compared numerically in terms of their basins of attraction with similar schemes without memory for finding multiple roots.
A Systematic Review of Case Reports on the Neck-Tongue Syndrome
Authors: Kim Hyunjoong , Lee Seungwon , Cho Namjeong , Song Seonghyeok
Published: October 2021

Background and Objectives: Neck-tongue syndrome (NTS) is rare, and characterized by unilateral upper neck or occipital pain and paresthesia in the ipsilateral hemisphere of the tongue due to neck movement. Treatment for NTS is mainly conservative, but the symptoms, causes, and rationale for treatment remain controversial. This study aimed to provide a framework for NTS treatment in clinical practice based on recent treatment directions. Materials and Methods: Case reports published from the past 20 years to August 2021 were searched through MEDLINE, EMBASE, and PEDro databases. Since there is no established management for NTS, the search terms were neck-tongue syndrome and case reports. The Critical Appraisal Checklist for Case Reports was used for the quality assessment of case reports. Through descriptive analysis, NTS symptoms, interventions, and results were reviewed. Results: Among the 16 studies searched, six case reports were selected and analyzed based on eight criteria. Symptoms included neck pain and ipsilateral tongue paralysis when the head was turned. As an intervention, six and four studies showed immediate symptom relief through manual therapy and exercise, respectively. Conclusions: Based on the reviewed evidence, management through physical therapy and chiropractic therapy with conservative methods such as manual therapy and exercise for patients with neck-tongue syndrome is recommended.
Targeting the Pentose Phosphate Pathway for SARS-CoV-2 Therapy
Authors: Bojkova Denisa , Costa Rui , Reus Philipp , Bechtel Marco , Jaboreck Mark-Christian , Olmer Ruth , Martin Ulrich , Ciesek Sandra , Michaelis Martin , Cinatl Jindrich
Published: October 2021

SARS-CoV-2 is causing the coronavirus disease 2019 (COVID-19) pandemic, for which effective pharmacological therapies are needed. SARS-CoV-2 induces a shift of the host cell metabolism towards glycolysis, and the glycolysis inhibitor 2-deoxy-d-glucose (2DG), which interferes with SARS-CoV-2 infection, is under development for the treatment of COVID-19 patients. The glycolytic pathway generates intermediates that supply the non-oxidative branch of the pentose phosphate pathway (PPP). In this study, the analysis of proteomics data indicated increased transketolase (TKT) levels in SARS-CoV-2-infected cells, suggesting that a role is played by the non-oxidative PPP. In agreement, the TKT inhibitor benfooxythiamine (BOT) inhibited SARS-CoV-2 replication and increased the anti-SARS-CoV-2 activity of 2DG. In conclusion, SARS-CoV-2 infection is associated with changes in the regulation of the PPP. The TKT inhibitor BOT inhibited SARS-CoV-2 replication and increased the activity of the glycolysis inhibitor 2DG. Notably, metabolic drugs like BOT and 2DG may also interfere with COVID-19-associated immunopathology by modifying the metabolism of immune cells in addition to inhibiting SARS-CoV-2 replication. Hence, they may improve COVID-19 therapy outcomes by exerting antiviral and immunomodulatory effects.
A Sensitivity-Enhanced Electrolyte-Gated Graphene Field-Effect Transistor Biosensor by Acoustic Tweezers
Authors: Chen Yan , Liu Wenpeng , Zhang Hao , Zhang Daihua , Guo Xiaoliang
Published: October 2021

Low-abundance biomolecule detection is very crucial in many biological and medical applications. In this paper, we present a novel electrolyte-gated graphene field-effect transistor (EGFET) biosensor consisting of acoustic tweezers to increase the sensitivity. The acoustic tweezers are based on a high-frequency bulk acoustic resonator with thousands of MHz, which has excellent ability to concentrate nanoparticles. The operating principle of the acoustic tweezers to concentrate biomolecules is analyzed and verified by experiments. After the actuation of acoustic tweezers for 10 min, the IgG molecules are accumulated onto the graphene. The sensitivities of the EGFET biosensor with accumulation and without accumulation are compared. As a result, the sensitivity of the graphene-based biosensor is remarkably increased using SMR as the biomolecule concentrator. Since the device has advantages such as miniaturized size, low reagent consumption, high sensitivity, and rapid detection, we expect it to be readily applied to many biological and medical applications.
Crystal Growth of Cubic and Hexagonal GaN Bulk Alloys and Their Thermal-Vacuum-Evaporated Nano-Thin Films
Authors: Fathy Marwa , Gad Sara , Anis Badawi , Kashyout Abd El-Hady B.
Published: October 2021

In this study, we investigate a novel simple methodology to synthesize gallium nitride nanoparticles (GaN) that could be used as an active layer in light-emitting diode (LED) devices by combining the crystal growth technique with thermal vacuum evaporation. The characterizations of structural and optical properties are carried out with different techniques to investigate the main featured properties of GaN bulk alloys and their thin films. Field emission scanning electron microscopy (FESEM) delivered images in bulk structures that show micro rods with an average diameter of 0.98 µm, while their thin films show regular microspheres with diameter ranging from 0.13 µm to 0.22 µm. X-ray diffraction (XRD) of the bulk crystals reveals a combination of 20% hexagonal and 80% cubic structure, and in thin films, it shows the orientation of the hexagonal phase. For HRTEM, these microspheres are composed of nanoparticles of GaN with diameter of 8–10 nm. For the optical behavior, a band gap of about from 2.33 to 3.1 eV is observed in both cases as alloy and thin film, respectively. This article highlights the fabrication of the major cubic structure of GaN bulk alloy with its thin films of high electron lifetime.
Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related ‘Omics Studies
Authors: Whitworth David E. , Sydney Natashia , Radford Emily J.
Published: October 2021

Myxobacteria are fascinating and complex microbes. They prey upon other members of the soil microbiome by secreting antimicrobial proteins and metabolites, and will undergo multicellular development if starved. The genome sequence of the model myxobacterium Myxococcus xanthus DK1622 was published in 2006 and 15 years later, 163 myxobacterial genome sequences have now been made public. This explosion in genomic data has enabled comparative genomics analyses to be performed across the taxon, providing important insights into myxobacterial gene conservation and evolution. The availability of myxobacterial genome sequences has allowed system-wide functional genomic investigations into entire classes of genes. It has also enabled post-genomic technologies to be applied to myxobacteria, including transcriptome analyses (microarrays and RNA-seq), proteome studies (gel-based and gel-free), investigations into protein–DNA interactions (ChIP-seq) and metabolism. Here, we review myxobacterial genome sequencing, and summarise the insights into myxobacterial biology that have emerged as a result. We also outline the application of functional genomics and post-genomic approaches in myxobacterial research, highlighting important findings to emerge from seminal studies. The review also provides a comprehensive guide to the genomic datasets available in mid-2021 for myxobacteria (including 24 genomes that we have sequenced and which are described here for the first time).
Strong Antibacterial Properties of Cotton Fabrics Coated with Ceria Nanoparticles under High-Power Ultrasound
Authors: Abramova Anna V. , Abramov Vladimir O. , Fedulov Igor S. , Baranchikov Alexander E. , Kozlov Daniil A. , Veselova Varvara O. , Kameneva Svetlana V. , Ivanov Vladimir K. , Cravotto Giancarlo
Published: October 2021

Flexible materials, such as fabric, paper and plastic, with nanoscale particles that possess antimicrobial properties have a significant potential for the use in the healthcare sector and many other areas. The development of new antimicrobial coating formulations is an urgent topic, as such materials could reduce the risk of infection in hospitals and everyday life. To select the optimal composition, a comprehensive analysis that takes into account all the advantages and disadvantages in each specific case must be performed. In this study, we obtained an antimicrobial textile with a 100% suppression of E. coli on its surface. These CeO2 nanocoatings exhibit low toxicity, are easy to manufacture and have a high level of antimicrobial properties even at very low CeO2 concentrations. High-power ultrasonic treatment was used to coat the surface of cotton fabric with CeO2 nanoparticles.
Direct Deviations in Astrocyte Free Ca2+ Concentration Control Multiple Arteriole Tone States
Authors: Haidey Jordan N. , Gordon Grant R.
Published: October 2021

Astrocytes elicit bidirectional control of microvascular diameter in acutely isolated brain slices through vasoconstriction and vasodilation pathways that can be differentially recruited via the free Ca2+ concentration in endfeet and/or the metabolic status of the tissue. However, the Ca2+-level hypothesis has not been tested using direct manipulation. To overcome this, we used Ca2+-clamp whole-cell patching of peri-arteriole astrocytes to change astrocyte-free Ca2+ to different concentrations and examined the vascular response. We discovered that clamping Ca2+ at the approximate resting value (100 nM) had no impact on arteriole diameter in a pre-constricted arteriole. However, a moderate elevation to 250 nM elicited sustained vasodilation that was blocked by the COX-1 antagonist SC-560 (500 nM). The vasodilation to 250 nM Ca2+ was sensitive to the metabolic state, as it converted to vasoconstriction when oxygen tension was dramatically elevated. In normal oxygen, clamping astrocyte Ca2+ well above the resting level (750 nM) produced sustained vasoconstriction, which converted to vasodilation in the 20-HETE blocker HET0016 (1 μM). This response was fully blocked by the addition of SC-560 (500 nM), showing that 20-HETE-induced vasoconstriction dominated the dilatory action of COX-1. These data demonstrate that direct changes in astrocyte free Ca2+ can control multiple arteriole tone states through different mediators.
Facial Emotion Recognition in Obesity and in Fibromyalgia: A Systematic Review
Authors: Vaioli Giulia , Scarpina Federica
Published: October 2021

Facial emotion recognition (FER) is extensively investigated in psychological sciences in healthy individuals and clinical conditions. In this paper, we analyzed those studies in which FER was assessed in the case of obesity or fibromyalgia, in relation to the levels of alexithymia. Crucially, these two conditions frequently co-occur; however, no study has explored FER considering both fibromyalgia and obesity. Studies were identified using the electronic search engine of PubMed. The last research was run on 23 July 2021. Two independent lists were generated for the two clinical conditions. Six records were reviewed about obesity, while three records about fibromyalgia. The evidence relative to FER in obesity was not conclusive, whereas the evidence about an altered FER in fibromyalgia seemed more straightforward. Moreover, the role of alexithymia on FER in these clinical conditions was not extensively investigated. In our discussion, we highlighted those factors that should be carefully addressed in investigating FER in these clinical conditions. Moreover, we underlined methodological criticisms that should be overcome in future research.
Distribution of Dissolved Nitrogen Compounds in the Water Column of a Meromictic Subarctic Lake
Authors: Vorobyeva Taisiya Ya. , Chupakova Anna A. , Chupakov Artem V. , Zabelina Svetlana A. , Moreva Olga Y. , Pokrovsky Oleg S.
Published: October 2021

In order to better understand the biogeochemical cycle of nitrogen in meromictic lakes, which can serve as a model for past aquatic environments, we measured dissolved concentrations of nitrate, nitrite, ammonium, and organic nitrogen in the deep (39 m maximal depth) subarctic Lake Svetloe (NW Russia). The lake is a rare type of freshwater meromictic water body with high concentrations of methane, ferrous iron, and manganese and low concentrations of sulfates and sulfides in the monimolimnion. In the oligotrophic mixolimnion, the concentration of mineral forms of nitrogen decreased in summer compared to winter, likely due to a phytoplankton bloom. The decomposition of the bulk of the organic matter occurs under microaerophilic/anaerobic conditions of the chemocline and is accompanied by the accumulation of nitrogen in the form of N-NH4 in the monimolimnion. We revealed a strong relationship between methane and nitrogen cycles in the chemocline and monimolimnion horizons. The nitrate concentrations in Lake Svetloe varied from 9 to 13 μM throughout the water column. This fact is rare for meromictic lakes, where nitrate concentrations up to 13 µM are found in the monimolimnion zone down to the bottom layers. We hypothesize, in accord with available data for other stratified lakes that under conditions of high concentrations of manganese and ammonium at the boundary of redox conditions and below, anaerobic nitrification with the formation of nitrate occurs. Overall, most of the organic matter in Lake Svetloe undergoes biodegradation essentially under microaerophilic/anaerobic conditions of the chemocline and the monimolimnion. Consequently, the manifestation of the biogeochemical nitrogen cycle is expressed in these horizons in the most vivid and complex relationship with other cycles of elements.
A Hypomethylating Ketogenic Diet in Apolipoprotein E-Deficient Mice: A Pilot Study on Vascular Effects and Specific Epigenetic Changes
Authors: Castro Rita , Whalen Courtney A. , Gullette Sean , Mattie Floyd J. , Florindo Cristina , Heil Sandra G. , Huang Neil K. , Neuberger Thomas , Ross A. Catharine
Published: October 2021

Hyperhomocysteneinemia (HHcy) is common in the general population and is a risk factor for atherosclerosis by mechanisms that are still elusive. A hypomethylated status of epigenetically relevant targets may contribute to the vascular toxicity associated with HHcy. Ketogenic diets (KD) are diets with a severely restricted amount of carbohydrates that are being widely used, mainly for weight-loss purposes. However, studies associating nutritional ketosis and HHcy are lacking. This pilot study investigates the effects of mild HHcy induced by nutritional manipulation of the methionine metabolism in the absence of dietary carbohydrates on disease progression and specific epigenetic changes in the apolipoprotein-E deficient (apoE–/–) mouse model. ApoE–/– mice were either fed a KD, a diet with the same macronutrient composition but low in methyl donors (low methyl KD, LMKD), or control diet. After 4, 8 or 12 weeks plasma was collected for the quantification of: (1) nutritional ketosis, (i.e., the ketone body beta-hydroxybutyrate using a colorimetric assay); (2) homocysteine by HPLC; (3) the methylating potential S-adenosylmethionine to S-adenosylhomocysteine ratio (AdoHcy/AdoMet) by LC-MS/MS; and (4) the inflammatory cytokine monocyte chemoattractant protein 1 (MCP1) by ELISA. After 12 weeks, aortas were collected to assess: (1) the vascular AdoHcy/AdoMet ratio; (2) the volume of atherosclerotic lesions by high-field magnetic resonance imaging (14T-MRI); and (3) the content of specific epigenetic tags (H3K27me3 and H3K27ac) by immunofluorescence. The results confirmed the presence of nutritional ketosis in KD and LMKD mice but not in the control mice. As expected, mild HHcy was only detected in the LMKD-fed mice. Significantly decreased MCP1 plasma levels and plaque burden were observed in control mice versus the other two groups, together with an increased content of one of the investigated epigenetic tags (H3K27me3) but not of the other (H3K27ac). Moreover, we are unable to detect any significant differences at the p < 0.05 level for MCP1 plasma levels, vascular AdoMet:AdoHcy ratio levels, plaque burden, and specific epigenetic content between the latter two groups. Nevertheless, the systemic methylating index was significantly decreased in LMKD mice versus the other two groups, reinforcing the possibility that the levels of accumulated homocysteine were insufficient to affect vascular transmethylation reactions. Further studies addressing nutritional ketosis in the presence of mild HHcy should use a higher number of animals and are warranted to confirm these preliminary observations.
Effect of Fermented Soymilk-Honey from Different Probiotics on Osteocalcin Level in Menopausal Women
Authors: Desfita Sri , Sari Wulan , Yusmarini Yusmarini , Pato Usman , Zakłos-Szyda Małgorzata , Budryn Grażyna
Published: October 2021

Osteoporosis has been discovered to be a risk factor for menopausal women. Although synbiotics (probiotics and prebiotics) are found in fermented soymilk-honey made using local probiotics, their effect on osteocalcin levels is still unknown. Therefore, this study’s objective was to determine the influence of fermented soymilk-honey from different probiotics on osteocalcin levels. A 90-day pre–post quasi-experimental study with a control design was conducted on 54 postmenopausal women divided into three intervention groups namely, the soymilk (SM) group, the soymilk-honey fermented with Lactobacillus casei subsp. casei R-68 (SMH Lc) group, and the soymilk-honey fermented with Lactobacillus plantarum 1 R 1.3.2 (SMH Lp) group. Participants consumed 100 mL of soymilk (SM) or fermented soymilk with honey (SMH Lc or SMH Lp) for 90 days. At the beginning and end of the study, the blood serum osteocalcin level was measured and subjects’ health status was assessed, such as cholesterol total, random blood glucose, and uric acid levels. Our results presented that in the SMH Lp group, 90 days supplementation of soy-honey milk fermented with Lactobacillus plantarum 1 R 1.3.2 significantly reduced the level of blood serum osteocalcin. Based on these results it is justified to perform more detailed studies on the effect of fermented soy-honey milk on bone health.
Type 2 Diabetes-Related Health Economic Impact Associated with Increased Whole Grains Consumption among Adults in Finland
Authors: Martikainen Janne , Jalkanen Kari , Heiskanen Jari , Lavikainen Piia , Peltonen Markku , Laatikainen Tiina , Lindström Jaana
Published: October 2021

The prevalence of type 2 diabetes (T2D) is increasing rapidly worldwide. A healthy diet supporting the control of energy intake and body weight has major importance in the prevention of T2D. For example, a high intake of whole grain foods (WGF) has been shown to be inversely associated with risk for T2D. The objective of the study was to estimate the expected health economic impacts of increased WGF consumption to decrease the incidence of T2D in the Finnish adult population. A health economic model utilizing data from multiple national databases and published scientific literature was constructed to estimate these population-level health economic consequences. Among the adult Finnish population, increased WGF consumption could reduce T2D-related costs between 286€ and 989€ million during the next 10-year time horizon depending on the applied scenario (i.e., a 10%-unit increase in a proportion of daily WGF users, an increased number (i.e., two or more) of WGF servings a day, or alternatively a combination of these scenarios). Over the next 20–30 years, a population-wide increase in WGF consumption could lead to much higher benefits. Furthermore, depending on the applied scenario, between 1323 and 154,094 quality-adjusted life years (QALYs) could be gained at the population level due to decreased T2D-related morbidity and mortality during the next 10 to 30 years. The results indicate that even when the current level of daily WGF consumption is already at a relatively high-level in a global context, increased WGF consumption could lead to important health gains and savings in the Finnish adult population.
Electrochemical Oxygen Sensors: A Preface to the Special Issue
Authors: de Sequeira César Augusto Correia
Published: October 2021

Chemical sensors are measurement devices that convert a chemical or physical property of a specific analyte into a measurable signal, whose magnitude is normally proportional to the concentration of the analyte [...]
Non-Vertical Exposures to HIV, HBV and HCV Infection in Children and Adolescents—Risk of Infection, Standards of Care and Postexposure Prophylaxis
Authors: Tomasik Anna , Pokorska-Śpiewak Maria , Marczyńska Magdalena
Published: October 2021

Introduction: in the review, we aimed to present current knowledge about the risk of infection, standards of care, and postexposure prophylaxis (PEP) in pediatric patients after non-vertical exposures to HIV, HBV, and HCV infection. Materials and Methods: the latest available literature and recommendations of Centers for Disease Control and Prevention (CDC), World Health Organization (WHO), European recommendations for the management of HIV and administration of non-occupational PEP, and Polish AIDS Society were reviewed. Results: the majority of cases of non-vertical exposure to blood-borne viruses in the pediatric population consist of sexual exposition and injection with unsterilized sharp objects (usually needlestick injuries). The risk HIV, HBV, and HCV transmission depend on several factors, and each exposure should be evaluated individually with consideration of the patient’s medical history. It is crucial to start antiretroviral therapy within 48 h from exposure. Treatment is continued for 28 days, and a 3-drugs regiment is recommended in the majority of cases. Decisions on hepatitis B and tetanus PEP are based on a history of vaccination. There is no PEP for hepatitis C infection, follow-up testing aims for early identification of disease and consideration of treatment options. Conclusion: all children after the non-vertical exposure to HIV, HBV, and HCV infection should be evaluated by the Infectious Disease specialist as soon as possible after the incident and qualified to post-exposure prophylaxis. Systematic diagnostic and follow-up on children after significant needlestick exposure should be maintained. Children after sexual exposure need a multidisciplinary approach. Response to reported event must be rapid and treatment must be comprehensive.
Recent Developments in the Synthesis of β-Diketones
Authors: de Gonzalo Gonzalo , Alcántara Andrés R.
Published: October 2021

Apart from being one of the most important intermediates in chemical synthesis, broadly used in the formation of C–C bonds among other processes, the β-dicarbonyl structure is present in a huge number of biologically and pharmaceutically active compounds. In fact, mainly derived from the well-known antioxidant capability associated with the corresponding enol tautomer, β-diketones are valuable compounds in the treatment of many pathological disorders, such as cardiovascular and liver diseases, hypertension, obesity, diabetes, neurological disorders, inflammation, skin diseases, fibrosis, or arthritis; therefore, the synthesis of these structures is an area of overwhelming interest for organic chemists. This paper is devoted to the advances achieved in the last ten years for the preparation of 1,3-diketones, using different chemical (Claisen, hydration of alkynones, decarboxylative coupling) or catalytic (biocatalysis, organocatalytic, metal-based catalysis) methodologies: Additionally, the preparation of branched β-dicarbonyl compounds by means of α-functionalization of non-substituted 1,3-diketones are also discussed.
A Strategy for Selective Deletion of Autoimmunity-Related T Cells by pMHC-Targeted Delivery
Authors: Goldberg Shalom D. , Felix Nathan , McCauley Michael , Eberwine Ryan , Casta Lou , Haskell Kathleen , Lin Tricia , Palovick Elizabeth , Klein Donna , Getts Lori , Getts Robert , Zhou Mimi , Bansal-Pakala Pratima , Dudkin Vadim
Published: October 2021

Autoimmune diseases such as rheumatoid arthritis are caused by immune system recognition of self-proteins and subsequent production of effector T cells that recognize and attack healthy tissue. Therapies for these diseases typically utilize broad immune suppression, which can be effective, but which also come with an elevated risk of susceptibility to infection and cancer. T cell recognition of antigens is driven by binding of T cell receptors to peptides displayed on major histocompatibility complex proteins (MHCs) on the cell surface of antigen-presenting cells. Technology for recombinant production of the extracellular domains of MHC proteins and loading with peptides to produce pMHCs has provided reagents for detection of T cell populations, and with the potential for therapeutic intervention. However, production of pMHCs in large quantities remains a challenge and a translational path needs to be established. Here, we demonstrate a fusion protein strategy enabling large-scale production of pMHCs. A peptide corresponding to amino acids 259–273 of collagen II was fused to the N-terminus of the MHC_II beta chain, and the alpha and beta chains were each fused to human IgG4 Fc domains and co-expressed. A tag was incorporated to enable site-specific conjugation. The cytotoxic drug payload, MMAF, was conjugated to the pMHC and potent, peptide-specific killing of T cells that recognize the collagen pMHC was demonstrated with tetramerized pMHC-MMAF conjugates. Finally, these pMHCs were incorporated into MMAF-loaded 3DNA nanomaterials in order to provide a biocompatible platform. Loading and pMHC density were optimized, and peptide-specific T cell killing was demonstrated. These experiments highlight the potential of a pMHC fusion protein-targeted, drug-loaded nanomaterial approach for selective delivery of therapeutics to disease-relevant T cells and new treatment options for autoimmune disease.
Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
Authors: Battisti Antonella , Morici Paola , Sgarbossa Antonella
Published: October 2021

Bacterial biofilm constitutes a strong barrier against the penetration of drugs and against the action of the host immune system causing persistent infections hardly treatable by antibiotic therapy. Helicobacter pylori (Hp), the main causative agent for gastritis, peptic ulcer and gastric adenocarcinoma, can form a biofilm composed by an exopolysaccharide matrix layer covering the gastric surface where the bacterial cells become resistant and tolerant to the commonly used antibiotics clarithromycin, amoxicillin and metronidazole. Antimicrobial PhotoDynamic Therapy (aPDT) was proposed as an alternative treatment strategy for eradicating bacterial infections, particularly effective for Hp since this microorganism produces and stores up photosensitizing porphyrins. The knowledge of the photophysical characteristics of Hp porphyrins in their physiological biofilm microenvironment is crucial to implement and optimize the photodynamic treatment. Fluorescence lifetime imaging microscopy (FLIM) of intrinsic bacterial porphyrins was performed and data were analyzed by the ‘fit-free’ phasor approach in order to map the distribution of the different fluorescent species within Hp biofilm. Porphyrins inside bacteria were easily distinguished from those dispersed in the matrix suggesting FLIM-phasor technique as a sensitive and rapid tool to monitor the photosensitizer distribution inside bacterial biofilms and to better orientate the phototherapeutic strategy.
Lipid Nanoparticles for Organ-Specific mRNA Therapeutic Delivery
Authors: Żak Magdalena M. , Zangi Lior
Published: October 2021

Advances in the using in vitro transcribed (IVT) modRNA in the past two decades, especially the tremendous recent success of mRNA vaccines against SARS-CoV-2, have brought increased attention to IVT mRNA technology. Despite its well-known use in infectious disease vaccines, IVT modRNA technology is being investigated mainly in cancer immunotherapy and protein replacement therapy, with ongoing clinical trials in both areas. One of the main barriers to progressing mRNA therapeutics to the clinic is determining how to deliver mRNA to target cells and protect it from degradation. Over the years, many different vehicles have been developed to tackle this issue. Desirable vehicles must be safe, stable and preferably organ specific for successful mRNA delivery to clinically relevant cells and tissues. In this review we discuss various mRNA delivery platforms, with particular focus on attempts to create organ-specific vehicles for therapeutic mRNA delivery.
Full-Vectorial Fiber Mode Solver Based on a Discrete Hankel Transform
Authors: Steinke Michael
Published: October 2021

It is crucial to be time and resource-efficient when enabling and optimizing novel applications and functionalities of optical fibers, as well as accurate computation of the vectorial field components and the corresponding propagation constants of the guided modes in optical fibers. To address these needs, a novel full-vectorial fiber mode solver based on a discrete Hankel transform is introduced and validated here for the first time for rotationally symmetric fiber designs. It is shown that the effective refractive indices of the guided modes are computed with an absolute error of less than 10−4 with respect to analytical solutions of step-index and graded-index fiber designs. Computational speeds in the order of a few seconds allow to efficiently compute the relevant parameters, e.g., propagation constants and corresponding dispersion profiles, and to optimize fiber designs.
Effect of Acid-Etching Duration on the Adhesive Performance of Printed Polyetheretherketone to Veneering Resin
Authors: Zhang Jiaqi , Yi Yingjie , Wang Chenwei , Ding Ling , Wang Ruijin , Wu Guofeng
Published: October 2021

Three-dimensional printing polyetheretherketone (PEEK) provides a new choice for dental prostheses, while its appropriate bonding procedure and adhesive performance are still unclear. This study aimed to investigate the adhesive performance of printed polyetheretherketone (PEEK) after acid etching to veneering resin. In total, 182 PEEK specimens (including 91 printed and 91 milled specimens) were distributed to 14 subgroups (n = 13/subgroup), according to the manufacturing process and surface treatment. The specimens were polished and etched with sulfuric acid for 0, 5, 30, 60, 90, 120, and 300 s, respectively. Two specimens in each subgroup were observed under a scanning electron microscope (SEM) for surface and cross-section morphology separately. Then, the specimens were treated with a bonding primer, and one specimen in each subgroup was prepared for cross-sectional observation under SEM. The residual 10 specimens of each subgroup bonded with veneering resin were tested with the shear bond strength tests (SBS) and failure modes analysis. Statistical analysis was performed by one-way ANOVA followed by the SNK-q post hoc test (p < 0.05). The etched pores on the PEEK surface were broadened and deepened under SEM over time. Printed PEEK etched for 30 s obtained the best SBS-to-veneering-resin ratio (27.90 ± 3.48 MPa) among the printed subgroups (p < 0.05) and had no statistical differences compared with milled PEEK etched for 30 s. The SBS of the milled subgroups etched from 5 to 120 s were over 29 MPa without significant between-group statistical differences. Hence, printed PEEK can be coarsened effectively by 30 s of sulfuric acid etching. The adhesion efficacy of printed PEEK to veneering resin was qualified for clinical requirements of polymer-based fixed dentures.