bluuebunny/crossref_metadata_2025 huggingface dataset:
Parental Selection of Preschool Programming in Saudi Arabia: A Qualitative Study
Authors: Abed Mohaned G. , Nahshal Maha M. , Shackelford Todd K.
Published: September 2022

A global initiative has emerged in recent years to highlight early childhood education and care as a foundation for later learning and development. The goal of this study was to investigate the choices that Saudi Arabian parents made for their children’s preschool education. In a qualitative, exploratory study, we investigated parental selection of preschool programming in Saudi Arabia. Guided by a phenomenological approach, we conducted semi-structured interviews with 12 Saudi Arabian women that had preschool-aged children. Although some research has examined the constraints and factors affecting parental selection of preschool programming, it remains unclear when these constraints and factors are considered by parents during the decision-making process, and how the decisions are made. The interviews revealed that parents consider not only educational reputation in the selection of preschool programming, but also instructional use of Arabic and English, religious foundation, teaching quality, classroom preparation, program scheduling, tuition costs, and physical security of the preschool environment. The results suggest that improving information collection is essential for empowering parents to make wise decisions, with the child’s happiness and academic growth having a significant impact on those decisions. The discussion addresses the importance of identifying the factors that affect parental selection of preschool programming, so that educational professionals may better meet the needs and expectations of parents in Saudi Arabia.
Comparative Analysis of 60Co and 192Ir Sources in High Dose Rate Brachytherapy for Cervical Cancer
Authors: Wen Aiping , Wang Xianliang , Wang Bingjie , Yan Chuanjun , Luo Jingyue , Wang Pei , Li Jie
Published: September 2022

High-dose-rate (HDR) brachytherapy (BT) is an essential treatment for cervical cancer, one of the most prevalent gynecological malignant tumors. In HDR BT, high radiation doses can be delivered to the tumor target with the minimum radiation doses to organs at risk. Despite the wide use of the small HDR 192Ir source, as the technique has improved, the HDR 60Co source, which has the same miniaturized geometry, has also been produced and put into clinical practice. Compared with 192Ir (74 days), 60Co has a longer half-life (5.3 years), which gives it a great economic advantage for developing nations. The aim of the study was to compare 60Co and 192Ir sources for HDR BT in terms of both dosimetry and clinical treatment. The results of reports published on the use of HDR BT for cervical cancer over the past few years as well as our own research show that this treatment is safe and it is feasible to use 60Co as an alternative source.
The Differential Paracrine Role of the Endothelium in Prostate Cancer Cells
Authors: Torres-Estay Verónica , Mastri Michalis , Rosario Spencer , Fuenzalida Patricia , Echeverría Carolina E. , Flores Emilia , Watts Anica , Cerda-Infante Javier , Montecinos Viviana P. , Sotomayor Paula C. , Amigo Julio , Escudero Carlos , Nualart Francisco , Ebos John M. L. , Smiraglia Dominic J. , Godoy Alejandro S.
Published: September 2022

The survival of patients with solid tumors, such as prostate cancer (PCa), has been limited and fleeting with anti-angiogenic therapies. It was previously thought that the mechanism by which the vasculature regulates tumor growth was driven by a passive movement of oxygen and nutrients to the tumor tissue. However, previous evidence suggests that endothelial cells have an alternative role in changing the behavior of tumor cells and contributing to cancer progression. Determining the impact of molecular signals/growth factors released by endothelial cells (ECs) on established PCa cell lines in vitro and in vivo could help to explain the mechanism by which ECs regulate tumor growth. Using cell-conditioned media collected from HUVEC (HUVEC-CM), our data show the stimulated proliferation of all the PCa cell lines tested. However, in more aggressive PCa cell lines, HUVEC-CM selectively promoted migration and invasion in vitro and in vivo. Using a PCa-cell-line-derived xenograft model co-injected with HUVEC or preincubated with HUVEC-CM, our results are consistent with the in vitro data, showing enhanced tumor growth, increased tumor microvasculature and promoted metastasis. Gene set enrichment analyses from RNA-Seq gene expression profiles showed that HUVEC-CM induced a differential effect on gene expression when comparing low versus highly aggressive PCa cell lines, demonstrating epigenetic and migratory pathway enrichments in highly aggressive PCa cells. In summary, paracrine stimulation by HUVEC increased PCa cell proliferation and tumor growth and selectively promoted migration and metastatic potential in more aggressive PCa cell lines.
PARP Inhibitors in Advanced Prostate Cancer in Tumors with DNA Damage Signatures
Authors: McNevin Ciara S. , Cadoo Karen , Baird Anne-Marie , Finn Stephen P. , McDermott Ray
Published: September 2022

Since 2010, significant progress has been made in the treatment of metastatic castrate resistant prostate cancer (mCRPC). While these advancements have improved survival, mCRPC remains a lethal disease, with a precision medicine framework that is lagging behind compared to other cancers. Poly (ADP-ribose) polymerase (PARP) inhibitor (PARPi) studies in prostate cancer (PCa) have focused primarily on the homologous recombination repair (HRR) genes, specifically BRCA1 and BRCA2. While homologous recombination deficiency (HRD) can be prompted by germline or somatic BRCA1/2 genetic mutations, it can also exist in tumors with intact BRCA1/BRCA2 genes. While the sensitivity of PARPi in tumors with non-BRCA DNA damage signatures is not as well established, it has been suggested that genomic alterations in DNA damage repair (DDR) genes other than BRCA may confer synthetic lethality with PARPI in mCRPC. The aim of this review is to summarize the literature on PARPi and their activity treating BRCA and non BRCA tumors with DNA damage signatures.
Episodic Memory and Recollection Network Disruptions Following Chemotherapy Treatment in Breast Cancer Survivors: A Review of Neuroimaging Findings
Authors: Bradley-Garcia Meenakshie , Winocur Gordon , Sekeres Melanie J.
Published: September 2022

Long-term memory disturbances are amongst the most common and disruptive cognitive symptoms experienced by breast cancer survivors following chemotherapy. To date, most clinical assessments of long-term memory dysfunction in breast cancer survivors have utilized basic verbal and visual memory tasks that do not capture the complexities of everyday event memories. Complex event memories, including episodic memory and autobiographical memory, critically rely on hippocampal processing for encoding and retrieval. Systemic chemotherapy treatments used in breast cancer commonly cause neurotoxicity within the hippocampus, thereby creating a vulnerability to memory impairment. We review structural and functional neuroimaging studies that have identified disruptions in the recollection network and related episodic memory impairments in chemotherapy-treated breast cancer survivors, and argue for the need to better characterize hippocampally mediated memory dysfunction following chemotherapy treatments. Given the importance of autobiographical memory for a person’s sense of identity, ability to plan for the future, and general functioning, under-appreciation of how this type of memory is impacted by cancer treatment can lead to overlooking or minimizing the negative experiences of breast cancer survivors, and neglecting a cognitive domain that may benefit from intervention strategies.
Transcriptional Profile Associated with Clinical Outcomes in Metastatic Hormone-Sensitive Prostate Cancer Treated with Androgen Deprivation and Docetaxel
Authors: Jiménez Natalia , Reig Òscar , Marín-Aguilera Mercedes , Aversa Caterina , Ferrer-Mileo Laura , Font Albert , Rodriguez-Vida Alejo , Climent Miguel Ángel , Cros Sara , Chirivella Isabel , Domenech Montserrat , Figols Mariona , González-Billalabeitia Enrique , Jiménez Peralta Daniel , Rodríguez-Carunchio Leonardo , García-Esteve Samuel , Garcia de Herreros Marta , Ribal Maria J. , Prat Aleix , Mellado Begoña
Published: September 2022

(1) Background: Androgen deprivation therapy (ADT) and docetaxel (DX) combination is a standard therapy for metastatic hormone-sensitive prostate cancer (mHSPC) patients. (2) Methods: We investigate if tumor transcriptomic analysis predicts mHSPC evolution in a multicenter retrospective biomarker study. A customized panel of 184 genes was tested in mRNA from tumor samples by the nCounter platform in 125 mHSPC patients treated with ADT+DX. Gene expression was correlated with castration-resistant prostate cancer-free survival (CRPC-FS) and overall survival (OS). (3) Results: High expression of androgen receptor (AR) signature was independently associated with longer CRPC-FS (hazard ratio (HR) 0.6, 95% confidence interval (CI) 0.3–0.9; p = 0.015), high expression of estrogen receptor (ESR) signature with longer CRPC-FS (HR 0.6, 95% CI 0.4–0.9; p = 0.019) and OS (HR 0.5, 95% CI 0.2–0.9, p = 0.024), and lower expression of tumor suppressor genes (TSG) (RB1, PTEN and TP53) with shorter OS (HR 2, 95% CI 1–3.8; p = 0.044). ARV7 expression was independently associated with shorter CRPC-FS (HR 1.5, 95% CI 1.1–2.1, p = 0.008) and OS (HR 1.8, 95% CI 1.2–2.6, p = 0.004), high ESR2 was associated with longer OS (HR 0.5, 95% CI 0.2–1, p = 0.048) and low expression of RB1 was independently associated with shorter OS (HR 1.9, 95% CI 1.1–3.2, p = 0.014). (4) Conclusions: AR, ESR, and TSG expression signatures, as well as ARV7, RB1, and ESR2 expression, have a prognostic value in mHSPC patients treated with ADT+DX.
Bacteriocins as Potential Therapeutic Approaches in the Treatment of Various Cancers: A Review of In Vitro Studies
Authors: Molujin Arnold Marshall , Abbasiliasi Sahar , Nurdin Armania , Lee Ping-Chin , Gansau Jualang Azlan , Jawan Roslina
Published: September 2022

Cancer is regarded as one of the most common and leading causes of death. Despite the availability of conventional treatments against cancer cells, current treatments are not the optimal treatment for cancer as they possess the possibility of causing various unwanted side effects to the body. As a result, this prompts a search for an alternative treatment without exhibiting any additional side effects. One of the promising novel therapeutic candidates against cancer is an antimicrobial peptide produced by bacteria called bacteriocin. It is a non-toxic peptide that is reported to exhibit potency against cancer cell lines. Experimental studies have outlined the therapeutic potential of bacteriocin against various cancer cell lines. In this review article, the paper focuses on the various bacteriocins and their cytotoxic effects, mode of action and efficacies as therapeutic agents against various cancer cell lines.
Echocardiography-Assessed Changes of Left and Right Ventricular Cardiac Function May Correlate with Progression of Advanced Lung Cancer—A Generating Hypothesis Study
Authors: Mędrek Sabina , Szmit Sebastian
Published: September 2022

Advanced lung cancer causes damage to lung tissue and the alveolar–capillary barrier, leading to changes in pulmonary circulation and cardiac function. This observational study included 75 patients with inoperable lung cancer. Two echocardiographic assessments were performed: one before the initiation of systemic anticancer therapy and another after the first radiological evaluation of the efficacy of anticancer treatment. In retrospective analysis, diagnosis of early cancer progression was associated significantly (p < 0.05) with some echocardiographic changes: a decrease in EF of at least 5 percentage points (OR = 5.78), an increase in LV GLS of 3 percentage points (OR = 3.81), an increase in E/E′ ratio of at least 3.25 (OR = 3.39), as well as a decrease in RV free wall GLS of at least 4 percentage points (OR = 4.9) and an increase in FAC of at least 4.1 percentage points (OR = 4.9). Cancer therapeutics-related cardiac dysfunction was diagnosed in accordance with the definition of the International Cardio-Oncology Society and was found more frequently in patients with radiologically confirmed lung cancer disease progression (p = 0.003). In further prospective studies, the hypothesis about the possible coexistence of the cardiotoxic effect of cancer therapy and cardiac dysfunction related to the progression of inoperable lung cancer should be clarified.
Cancer-Related Symptom Management Intervention for Southwest American Indians
Authors: Hodge Felicia S. , Line-Itty Tracy , Arbing Rachel H. A.
Published: September 2022

There is limited literature related to culturally embedded meanings of cancer and related symptoms among American Indians. A culturally appropriate intervention to improve management of cancer-related symptoms, including pain, depression, fatigue and loss of function, was tested. Two-hundred and twenty-two adult American Indians with cancer were recruited from eight Southwest sites for a randomized clinical trial. The intervention group received tailored education, a toolkit with a video, and participated in discussion sessions on cancer symptom management; the control group received information on dental care. Pre- and post-test questionnaires were administered to control and intervention groups. Measures included socio-demographics, cancer-related symptom management knowledge and behavior, and quality of life measures. Male cancer survivors reported poorer self-assessed health status and lower scores on quality-of-life indicators as compared to female cancer survivors. Significant improvement was reported in symptom management knowledge scores following the intervention: management of pain (p = 0.003), depression (p = 0.004), fatigue (p = 0.0001), and loss of function (p = 0.0001). This study is one of the first to demonstrate a change in physical symptom self-management skills, suggesting culturally appropriate education and interventions can successfully enhance cancer-related symptom management knowledge and practice.
Full Blood Count Trends for Colorectal Cancer Detection in Primary Care: Development and Validation of a Dynamic Prediction Model
Authors: Virdee Pradeep S. , Patnick Julietta , Watkinson Peter , Holt Tim , Birks Jacqueline
Published: September 2022

Colorectal cancer has low survival rates when late-stage, so earlier detection is important. The full blood count (FBC) is a common blood test performed in primary care. Relevant trends in repeated FBCs are related to colorectal cancer presence. We developed and internally validated dynamic prediction models utilising trends for early detection. We performed a cohort study. Sex-stratified multivariate joint models included age at baseline (most recent FBC) and simultaneous trends over historical haemoglobin, mean corpuscular volume (MCV), and platelet measurements up to baseline FBC for two-year risk of diagnosis. Performance measures included the c-statistic and calibration slope. We analysed 250,716 males and 246,695 females in the development cohort and 312,444 males and 462,900 females in the validation cohort, with 0.4% of males and 0.3% of females diagnosed two years after baseline FBC. Compared to average population trends, patient-level declines in haemoglobin and MCV and rise in platelets up to baseline FBC increased risk of diagnosis in two years. C-statistic: 0.751 (males) and 0.763 (females). Calibration slope: 1.06 (males) and 1.05 (females). Our models perform well, with low miscalibration. Utilising trends could bring forward diagnoses to earlier stages and improve survival rates. External validation is now required.
Review of Under-Recognized Adjunctive Therapies for Cancer
Authors: Money Mary E. , Matthews Carolyn M. , Tan-Shalaby Jocelyn
Published: September 2022

Patients and providers may not be aware that several adjunctive measures can significantly improve the quality of life, response to treatment, and possibly outcomes for cancer patients. This manuscript presents a review of practical under-recognized adjunctive therapies that are effective including exercise; stress-reduction techniques such as mindfulness, massage, yoga, Tai Chi, breathing exercises; importance of sleep quality; diet modifications such as calorie restriction at the time of chemotherapy and avoidance of high carbohydrate foods; supplements such as aspirin, green tea, turmeric, and melatonin; and repurposed prescription medications such as metformin and statins. Each recommendation should be tailored to the individual patient to assure no contraindications.
Secrets of Flavonoid Synthesis in Mushroom Cells
Authors: Pukalski Jan , Latowski Dariusz
Published: September 2022

Flavonoids are chemical compounds that occur widely across the plant kingdom. They are considered valuable food additives with pro-health properties, and their sources have also been identified in other kingdoms. Especially interesting is the ability of edible mushrooms to synthesize flavonoids. Mushrooms are usually defined as a group of fungal species capable of producing macroscopic fruiting bodies, and there are many articles considering the content of flavonoids in this group of fungi. Whereas the synthesis of flavonoids was revealed in mycelial cells, the ability of mushroom fruiting bodies to produce flavonoids does not seem to be clearly resolved. This article, as an overview of the latest key scientific findings on flavonoids in mushrooms, outlines and organizes the current state of knowledge on the ability of mushroom fruiting bodies to synthesize this important group of compounds for vital processes. Putting the puzzle of the current state of knowledge on flavonoid biosynthesis in mushroom cells together, we propose a universal scheme of studies to unambiguously decide whether the fruiting bodies of individual mushrooms are capable of synthesizing flavonoids.
Mitochondrion-Mediated Cell Death through Erk1-Alox5 Independent of Caspase-9 Signaling
Authors: Chen Min , Wang Lei , Li Min , Budai Marietta M. , Wang Jin
Published: September 2022

Mitochondrial disruption leads to the release of cytochrome c to activate caspase-9 and the downstream caspase cascade for the execution of apoptosis. However, cell death can proceed efficiently in the absence of caspase-9 following mitochondrial disruption, suggesting the existence of caspase-9-independent cell death mechanisms. Through a genome-wide siRNA library screening, we identified a network of genes that mediate caspase-9-independent cell death, through ROS production and Alox5-dependent membrane lipid peroxidation. Erk1-dependent phosphorylation of Alox5 is critical for targeting Alox5 to the nuclear membrane to mediate lipid peroxidation, resulting in nuclear translocation of cytolytic molecules to induce DNA damage and cell death. Consistently, double knockouts of caspase-9 and Alox5 in mice, but not deletion of either gene alone, led to significant T cell expansion with inhibited cell death, indicating that caspase-9- and Alox5-dependent pathways function in parallel to regulate T cell death in vivo. This unbiased whole-genome screening reveals an Erk1-Alox5-mediated pathway that promotes membrane lipid peroxidation and nuclear translocation of cytolytic molecules, leading to the execution of cell death in parallel to the caspase-9 signaling cascade.
The Green Valley of Drosophila melanogaster Constitutive Heterochromatin: Protein-Coding Genes Involved in Cell Division Control
Authors: Messina Giovanni , Prozzillo Yuri , Bizzochi Greta , Marsano Renè Massimiliano , Dimitri Patrizio
Published: September 2022

Constitutive heterochromatin represents a significant fraction of eukaryotic genomes (10% in Arabidopsis, 20% in humans, 30% in D. melanogaster, and up to 85% in certain nematodes) and shares similar genetic and molecular properties in animal and plant species. Studies conducted over the last few years on D. melanogaster and other organisms led to the discovery of several functions associated with constitutive heterochromatin. This made it possible to revise the concept that this ubiquitous genomic territory is incompatible with gene expression. The aim of this review is to focus the attention on a group of protein-coding genes resident in D. melanogaster constitutive of heterochromatin, which are implicated in different steps of cell division.
Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
Authors: Olakowska Edyta , Rodak Piotr , Pacwa Anna , Machowicz Joanna , Machna Bartosz , Lewin-Kowalik Joanna , Smedowski Adrian
Published: September 2022

Deficiency of estradiol during the menopausal period is an important risk factor for neurodegenerative diseases, including various optic neuropathies. The aim of this study was to evaluate the impact of surgical menopause on the function and survival ratio of RGCs in the rat model of ONC (optic nerve crush). We used eight-week-old female Long Evans rats, divided into two main groups depending on the time between ovariectomy procedure (OVA) and euthanasia (two weeks vs. seven weeks), and subgroups—OVA, OVA + ONC, or ONC. Retinal function was assessed with electroretinography (ERG). RGC loss ratio was evaluated using immunolabelling and counting of RGCs. Seven weeks after OVA, the menopause morphologically affected interneurons but not RGC; however, when the ONC procedure was applied, RGCs appeared to be more susceptible to damage in case of deprivation of estrogens. In our analysis, PhNR (photopic negative responses) were severely diminished in the OVA + ONC group. A deprivation of estrogens in menopause results in accelerated retinal neurodegeneration that firstly involves retinal interneurons. The lack of estrogens increases the susceptibility of RGCs to insults.
Restored Fyn Levels in Huntington’s Disease Contributes to Enhanced Synaptic GluN2B-Composed NMDA Receptors and CREB Activity
Authors: Fão Lígia , Coelho Patrícia , Rodrigues Ricardo J. , Rego A. Cristina
Published: September 2022

N-methyl-D-aspartate receptors (NMDARs) are important postsynaptic receptors that contribute to normal synaptic function and cell survival; however, when overactivated, as in Huntington’s disease (HD), NMDARs cause excitotoxicity. HD-affected striatal neurons show altered NMDAR currents and augmented ratio of surface to internal GluN2B-containing NMDARs, with augmented accumulation at extrasynaptic sites. Fyn protein is a member of the Src kinase family (SKF) with an important role in NMDARs phosphorylation and synaptic localization and function; recently, we demonstrated that Fyn is reduced in several HD models. Thus, in this study, we aimed to explore the impact of HD-mediated altered Fyn levels at post-synaptic density (PSD), and their role in distorted NMDARs function and localization, and intracellular neuroprotective pathways in YAC128 mouse primary striatal neurons. We show that reduced synaptic Fyn levels and activity in HD mouse striatal neurons is related to decreased phosphorylation of synaptic GluN2B-composed NMDARs; this occurs concomitantly with augmented extrasynaptic NMDARs activity and currents and reduced cAMP response element-binding protein (CREB) activation, along with induction of cell death pathways. Importantly, expression of a constitutive active form of SKF reestablishes NMDARs localization, phosphorylation, and function at PSD in YAC128 mouse neurons. Enhanced SKF levels and activity also promotes CREB activation and reduces caspase-3 activation in YAC128 mouse striatal neurons. This work supports, for the first time, a relevant role for Fyn protein in PSD modulation, controlling NMDARs synaptic function in HD, and favoring neuroprotective pathways and cell survival. In this respect, Fyn Tyr kinase constitutes an important potential HD therapeutic target directly acting at PSD.
CUEDC2 Drives β-Catenin Nuclear Translocation and Promotes Triple-Negative Breast Cancer Tumorigenesis
Authors: Han Shuyan , Hao Huifeng , Han Haibo , Xue Dong , Jiao Yanna , Xie Yuntao , Xu Ye , Huangfu Longtao , Fu Jialei , Wang Shan , Sun Hong , Li Pingping , Zhou Qun
Published: September 2022

Hyperactivation of Wnt signaling is crucial in tumor formation. Fully elucidating the molecular details of how the cancer-specific Wnt signaling pathway is activated or contributes to tumorigenesis will help in determining future treatment strategies. Here, we aimed to explore the contribution of CUEDC2, a novel CUE-domain-containing protein, to the activation of Wnt signaling and the tumorigenesis of triple-negative breast cancer (TNBC) and to determine the underlying mechanisms. TNBC patient samples and disease-free survival (DFS) data were used to determine the association between CUEDC2 and TNBC progression. The effects of CUEDC2 on TNBC were examined in TNBC cells in vitro and in subcutaneous xenograft tumors in vivo. Gene knockdown, immunoprecipitation plus liquid chromatography–tandem mass spectrometry, pull-down, co-immunoprecipitation, localized surface plasmon resonance, and nuclear translocation analysis were used to uncover the mechanisms of CUEDC2 in regulating Wnt signaling and TNBC development. CUEDC2 is sufficient to maintain the hyperactivation of Wnt signaling required for TNBC tumorigenesis. The contribution of CUEDC2 plays a major role in determining the outcome of oncogenic Wnt signaling both in vitro and in vivo. Mechanistically, the CUE domain in CUEDC2 directly bound to the ARM (7–9) domain in β-catenin, promoted β-catenin nuclear translocation and enhanced the expression of β-catenin targeted genes. More importantly, an 11-amino-acid competitive peptide targeting the CUE domain in CUEDC2 blocked the interactions of CUEDC2 and β-catenin and abrogated the malignant phenotype of TNBC cells in vitro and in vivo. We observed that TNBC patients who exhibited higher levels of CUEDC2 showed marked hyperactivation of the Wnt signaling pathway and poor clinical outcomes, highlighting the clinical relevance of our findings. CUEDC2 promotes TNBC tumor growth by enhancing Wnt signaling through directly binding to β-catenin and accelerating its nuclear translocation. Targeting the interactions of CUEDC2 and β-catenin may be a valuable strategy for combating TNBC.
Synthesis and In Silico Study of Some New bis-[1,3,4]thiadiazolimines and bis-Thiazolimines as Potential Inhibitors for SARS-CoV-2 Main Protease
Authors: Gomha Sobhi M. , Riyadh Sayed M. , Abdellattif Magda H. , Abolibda Tariq Z. , Abdel-aziz Hassan M. , Nayl AbdElAziz. A. , Elgohary Alaa M. , Elfiky Abdo A.
Published: September 2022

A novel series of bis-[1,3,4]thiadiazolimines, and bis-thiazolimines, with alkyl linker, were synthesized through general routes from cyclization of 1,1′-(hexane-1,6-diyl)bis(3-phenylthiourea) and hydrazonoyl halides or α-haloketones, respectively. Docking studies were applied to test the binding affinity of the synthesized products against the Mpro of SARS-CoV-2. The best compound, 5h, has average binding energy (−7.50 ± 0.58 kcal/mol) better than that of the positive controls O6K and N3 (−7.36 ± 0.34 and −6.36 ± 0.31 kcal/mol). Additionally, the docking poses (H-bonds and hydrophobic contacts) of the tested compounds against the Mpro using the PLIP web server were analyzed.
Mg Corrosion—Recent Progress
Authors: Atrens Andrej , Chen Xingrui , Shi Zhiming
Published: September 2022

Recent progress is reviewed. Recent developments include: (i) accumulation of evidence that electrochemical measurements of the Mg corrosion rate often do not agree with the steady state Mg corrosion rate as measured by weight loss; (ii) low Fe tolerance limits are caused by heat treatment of nominally high-purity Mg and the presence of Si, (iii) the intrinsic Mg corrosion rate is 0.3 mm/y in a chloride solution as measured by weight loss, (iv) there are many Mg alloys with corrosion rates between 0.3 and 1.0 mm/y, (v) there are few Mg alloys with corrosion rates less than 0.3 mm/y, (vi) experimental evidence contradicts the enhanced catalytic activity mechanism of Mg corrosion, (vii) experiments support the uni-positive Mg+ mechanism, (viii) new compelling experimental evidence supporting the uni-positive Mg+ corrosion mechanism has been provided by electrochemical impedance spectroscopy (EIS), and (ix) the uni-positive Mg+ corrosion mechanism provides new insights for understanding the performance of Mg-air batteries and for the development of better Mg anodes.
Measurement and Interpretation of the Effect of Electrical Sliding Speed on Contact Characteristics of On-Load Tap Changers
Authors: Yang Xingzu , Li Shuaibing , Cui Yi , Kang Yongqiang , Li Zongying , Li Hongwei , Dong Haiying
Published: September 2022

This paper analyzes the effect of sliding speed on the electrical conductivity and friction properties of the contact pair of an on-load tap changer (OLTC). Reciprocating current-carrying tribological tests were carried out on a rod–plate–copper–tin–copper contact galvanic couple at different sliding speeds in air and insulating oil media. The results show that as the sliding speed increases from 24 mm/s to 119 mm/s, the average contact resistance in air increases from 0.2 Ω to 0.276 Ω, and the average contact resistance in insulating oil also increases from 0.2 Ω to 0.267 Ω. At 119 mm/s, the maximum contact resistance in insulating oil reaches 0.3 Ω. The micro-topography images obtained by scanning electron microscopy show that with the increase in sliding speed, the wear mechanisms in the air are mainly abrasive wear and adhesive wear, and the wear mechanisms in oil are mainly layered wear and erosion craters; high sliding speed and arcing promote contact surface fatigue and crack generation. X-ray photoelectron spectroscopy was used to analyze the surface. The copper oxide in the air and the cuprous sulfide in the insulating oil cause the surface film resistance, and the total contact resistance increases accordingly. In addition, the test shows that 119 mm/s in air and 95 mm/s in insulating oil are the speed thresholds. Below these speed thresholds, the increase in contact resistance is mainly caused by mechanical wear. Above these thresholds, the increase in contact resistance is mainly caused by arc erosion and chemical oxidation processes. Non-mechanical factors exacerbate the deterioration of the contact surface and become the main factor for the increase in contact resistance.
External Validation of the Graded Prognostic Assessment in Patients with Brain Metastases from Small Cell Lung Cancer
Authors: Nieder Carsten , Popp Ilinca , Hintz Mandy , Grosu Anca Ligia
Published: September 2022

Background: Recently, graded prognostic assessment (GPA) for small cell lung cancer (SCLC) patients with brain metastases has been developed. This includes age, performance status, number of brain metastases and presence of extracranial metastases. The aim of the present study was to validate this four-tiered prognostic score in a European cohort of patients. Methods: The retrospective validation study included 180 patients from two centers in Germany and Norway. Results: Median survival from radiological diagnosis of brain metastases was 7 months. The GPA point sum as continuous variable (0–4 points) was significantly associated with survival (p < 0.001). However, no significant survival difference was observed between patients in the two strata with better survival (3.5–4 and 2.5–3 points, respectively). Long-term survival in the poor prognosis group (0–1 points) was better than expected. Conclusion: This study supports the prognostic impact of all four parameters contributing to the GPA. The original way of grouping the parameters and breaking the final strata did not give optimal results in this cohort. Therefore, additional validation databases from different countries should be created and evaluated.
Proteomic Analysis of the Seeds of Four Wild Mexican Lupinus Species: Focus on Storage Proteins
Authors: Xu Ruoxian , Sirtori Elena , Boschin Giovanna , Torres Kalina Bermudez , Arnoldi Anna , Aiello Gilda
Published: September 2022

Lupinus is a wide genus, comprising between 300 and 500 species, most of them represented in America. Mexico is a secondary distribution center with more than 100 species growing along the highlands. Due to morphological similarities, the taxonomy of wild Lupinus species is still incomplete. It is, therefore, useful to collect morphological, chemical, and molecular data for the correct differentiation of these plants. In the present work, the composition of the seed proteins of four species: Lupinus aschenbornii Schauer, Lupinus campestris Cham and Schlecht, Lupinus hintonii C.P. Smith, and Lupinus montanus Kunth were analyzed. Seeds were collected at Iztaccihuatl—Popocatepetl National Park. Both total proteins and single protein families, purified by chromatographic procedures, were analyzed by SDS-PAGE and 2D-electrophoresis and by LC-MS/MS analysis. Data were compared with those of domesticated species whose proteomes had been already described in the literature. The protein profile may be useful for species identification since they have specific characteristics in each single species.
The Rapid Expansion of the Jumping Snail Ovachlamys fulgens in Brazil
Authors: Rosa Rafael Masson , Salvador Rodrigo Brincalepe , Teixeira Larissa , Bornschein Marcos Ricardo , Cavallari Daniel Caracanhas
Published: September 2022

The exotic Japanese jumping snail, Ovachlamys fulgens, was first recorded in Santa Catarina state, Brazil, in 2013. Based on data gathered from the literature, natural history collections, field samplings, and the iNaturalist platform, we assess its current distribution in the country. Our data show that the jumping snail has had a dramatic range expansion since its introduction, reaching six other states from the southern region of the country toward the Midwest. The affected locations are mostly urban but also include three biological reserves and protected areas, and possibly more. We discuss the consequences of this rapid invasion, its means of introduction, its local ecology, and prospects for monitoring and control.
Living in the Extreme: Fatty Acid Profiles and Their Specificity in Certain Tissues of Dominant Antarctic Silverfish, Pleuragramma antarcticum, from the Antarctic Sound (Southern Ocean) Collected during the Austral Summer
Authors: Pekkoeva Svetlana N. , Voronin Viktor P. , Mishin Aleksej V. , Orlov Alexei M. , Murzina Svetlana A.
Published: September 2022

The fatty acid profile of seven different organs (brain, gills, heart, muscles, gonads, liver, and gall bladder) of the lipid-rich Antarctic silverfish, Pleuragramma antarcticum, from the Antarctic Sound was studied. Qualitative and quantitative analyses of fatty acids were performed using gas chromatography with mass-selective and flame ionization detectors. It was seen that the muscles and gills differed from the other organs with the prevalence of saturated fatty acids, mainly due to 16:0 and 14:0 that may reveal their additional metabolic role in these organs for adaptation of the fish to the cold Antarctic waters. Monounsaturated fatty acids 18:1(n − 9) and 16:1(n − 7) were prevalent in the other organs of silverfish, especially their high content in the liver, reflecting the carnivorous feeding type (mainly adult ice krill) of the species and their dietary preferences in the austral summer. The high content of essential fatty acids, 22:6(n − 3) (14% of total fatty acids) found in the brain and 20:5(n − 3) (12% of total fatty acids) found in the muscles, highlights the functional significance of these lipid components in the studied tissues of fish. The results will improve the understanding of the functional value of individual fatty acids in terms of their organ specificity and the adaptation of the Antarctic silverfish to life in the frigid ice waters of the Antarctic pelagic ecosystem.
Bat Species Diversity and Abundance of Trophic Guilds after a Major Hurricane along an Anthropic Disturbance Gradient
Authors: Sil-Berra Luz María , Sánchez-Hernández Cornelio , Romero-Almaraz María de Lourdes , Reynoso Víctor Hugo
Published: September 2022

The frequency and intensity of hurricanes have increased with climate change, and their effects on most taxa are not known. We analyzed a species diversity of bats in three locations with different regimes of anthropic disturbance. We assessed the effect of the season and post-hurricane time on the abundance of trophic guilds in coastal Jalisco, México, during the two years following Hurricane Patricia (category 4). During a sampling effort of 15,629.76 m2 of netting, we captured 790 bats of 21 species. The species diversity was higher in the site with the highest proportion of primary tropical deciduous forest and was higher in 2016 than in 2017; the species composition did not differ greatly between the two years. The abundance of bats in various trophic guilds varied relative to the four climatic seasons. The general abundance of bats, frugivores-omnivores, and insectivores showed a significant increasing trend over time after the hurricane, which may indicate a recovery of the ecosystem or an abundance of early-successional fruiting plants. The results also confirm that species diversity recovers faster in a conserved forest. Thus, it is important to conserve natural areas to mitigate the effects of major disturbances.
The Additional Value of Lower Respiratory Tract Sampling in the Diagnosis of COVID-19: A Real-Life Observational Study
Authors: Morandi Luca , Torsani Francesca , Forini Giacomo , Tamburrini Mario , Carnevale Aldo , Pecorelli Anna , Giganti Melchiore , Piattella Marco , Guzzinati Ippolito , Papi Alberto , Contoli Marco
Published: September 2022

Background: Since December 2019, SARS-CoV-2 has been causing cases of severe pneumonia in China and has spread all over the world, putting great pressure on health systems. Nasopharyngeal swab (NPS) sensitivity is suboptimal. When the SARS-CoV-2 infection is suspected despite negative NPSs, other tests may help to rule out the infection. Objectives: To evaluate the yield of the lower respiratory tract (LRT) isolation of SARS-CoV-2. To evaluate the correlations between SARS-CoV-2 detection and clinical symptoms, and laboratory values and RSNA CT review scores in suspect patients after two negative NPSs. To assess the safety of bronchoscopy in this scenario. Method: A retrospective analysis of data from LRT sampling (blind nasotracheal aspiration or bronchial washing) for suspected COVID-19 after two negative NPS. Chest CT scans were reviewed by two radiologists using the RSNA imaging classification. Results: SARS-CoV-2 was detected in 14/99 patients (14.1%). A correlation was found between SARS-CoV2 detection on the LRT and the presence of a cough as well as with typical CT features. Typical CT resulted in 57.1% sensitivity, 80.8% accuracy and 92.3% NPV. Neither severe complications nor infections in the personnel were reported. Conclusions: In suspect cases after two negative swabs, CT scan revision can help to rule out COVID-19. In selected cases, with consistent CT features above all, LRT sampling can be of help in confirming COVID-19.
Application of Ultrasound Elastography in Assessing Portal Hypertension
Authors: Zhang Man , Jin Hongyu , Cao Jiazhi , Ren Ruyu , Jia Menglu , Yang Yi , Li Xinyi , Chen Ming , Li Shen , Huang Libin , Ling Wenwu
Published: September 2022

Portal hypertension is a common manifestation in late-to-end-stage liver diseases and can cause severe complications such as ascites, hepatic encephalopathy, etc. However, an early diagnosis of portal hypertension is often difficult as it can be asymptomatic. Though the gold standard to diagnose portal hypertension is hepatic vein catheterization, ultrasound elastography is regarded as a noninvasive alternative that can be used to accurately predict portal hypertension and a few further complications such as gastro-esophageal varices. Since ultrasound elastography is available in most medical centers, and is cheaper and noninvasive, studying its function in predicting portal hypertension is of paramount importance. Therefore, this review generalized the results of recently published articles in order to establish the indicators that were related to diagnostic and prediction efficiency. Our study found that various technologies of ultrasound elastography could be used to predict portal hypertension with satisfactory diagnostic sensitivity, specificity, accuracy, and AUC. Meanwhile, we also recognized similar diagnostic efficiency of ultrasound elastography in gastro-esophageal varices.
Explicit and Implicit Trust Modeling for Recommendation
Authors: Demirci Utku , Karagoz Pinar
Published: September 2022

Recommendation has become an inseparable component of many software applications, such as e-commerce, social media and gaming platforms. Particularly in collaborative filtering-based recommendation solutions, the preferences of other users are considered heavily. At this point, trust among the users comes into the scene as an important concept to improve the recommendation performance. Trust describes the nature and the strength of ties between individuals and hence provides useful information to improve the recommendation accuracy, particularly against data sparsity and cold start problems. The Trust notion helps alleviate the effect of these problems by providing additional reliable relationships between the users. However, trust information, specifically explicit trust, is not straightforward to collect and is only scarcely available. Therefore, implicit trust models have been proposed to fill in the gap. The literature includes a variety of studies proposing the use of trust for recommendation. In this work, two specific sub-problems are elaborated on: the relationship between explicit and implicit trust scores, and the construction of a machine learning model for explicit trust. For the first sub-problem, an implicit trust model is devised and the compatibility of implicit trust scores with explicit scores is analyzed. For the second sub-problem, two different explicit trust models are proposed: Explicit trust modeling through users’ rating behavior and explicit trust modeling as a link prediction problem. The performances of the prediction models are analyzed on a set of benchmark data sets. It is observed that explicit and implicit trust models have different natures, and are to be used in a complementary way for recommendation. Another important result is that the accuracy of the machine learning models for explicit trust is promising and depends on the availability of data.
Preparation and Analysis of Two-Dimensional Four-Qubit Entangled States with Photon Polarization and Spatial Path
Authors: Zhao Jiaqiang , Wang Meijiao , Sun Bing , Cao Lianzhen , Yang Yang , Liu Xia , Zhang Qinwei , Lu Huaixin , Driscoll Kellie Ann
Published: September 2022

Entanglement states serve as the central resource for a number of important applications in quantum information science, including quantum key distribution, quantum precision measurement, and quantum computing. In pursuit of more promising applications, efforts have been made to generate entangled states with more qubits. However, the efficient creation of a high-fidelity multiparticle entanglement remains an outstanding challenge due to the difficulty that increases exponentially with the number of particles. We design an interferometer that is capable of coupling the polarization and spatial paths of photons and prepare 2-D four-qubit GHZ entanglement states. Using quantum state tomography, entanglement witness, and the violation of Ardehali inequality against local realism, the properties of the prepared 2-D four-qubit entangled state are analyzed. The experimental results show that the prepared four-photon system is an entangled state with high fidelity.
Statistical Equilibrium Principles in 2D Fluid Flow: From Geophysical Fluids to the Solar Tachocline
Authors: Weichman Peter B. , Marston John Bradley
Published: September 2022

An overview is presented of several diverse branches of work in the area of effectively 2D fluid equilibria which have in common that they are constrained by an infinite number of conservation laws. Broad concepts, and the enormous variety of physical phenomena that can be explored, are highlighted. These span, roughly in order of increasing complexity, Euler flow, nonlinear Rossby waves, 3D axisymmetric flow, shallow water dynamics, and 2D magnetohydrodynamics. The classical field theories describing these systems bear some resemblance to perhaps more familiar fluctuating membrane and continuous spin models, but the fluid physics drives these models into unconventional regimes exhibiting large scale jet and eddy structures. From a dynamical point of view these structures are the end result of various conserved variable forward and inverse cascades. The resulting balance between large scale structure and small scale fluctuations is controlled by the competition between energy and entropy in the system free energy, in turn highly tunable through setting the values of the conserved integrals. Although the statistical mechanical description of such systems is fully self-consistent, with remarkable mathematical structure and diversity of solutions, great care must be taken because the underlying assumptions, especially ergodicity, can be violated or at minimum lead to exceedingly long equilibration times. Generalization of the theory to include weak driving and dissipation (e.g., non-equilibrium statistical mechanics and associated linear response formalism) could provide additional insights, but has yet to be properly explored.
Decreasing the Negative Impact of Time Delays on Electricity Due to Performance Improvement in the Rwanda National Grid
Authors: Muyizere Darius , Letting Lawrence K. , Munyazikwiye Bernard B.
Published: September 2022

One of the most common power problems today is communication and control delays. This can adversely affect decision interaction in grid security management. This paper focuses on communication signal delays and how to identify and address communication system failure issues in the context of grid monitoring and control, with emphasis on communication signal delay. An application to solve this problem uses a thyristor switch capacitor (TSC) and a thyristor-controlled reactor (TCR) to improve the power quality of the Rwandan National Grid (RNG) with synchronous and PV generators. It is to counteract the negative effects of time delays. To this end, the TSC and TCR architectures use two methods: the fuzzy logic controller (FLC) method and the modified predictor method (MPM). The experiment was performed using the Simulink MATLAB tool. The power quality of the system was assessed using two indicators: the voltage index and total harmonic distortion. The FLC-based performance was shown to outperform the MPM for temporary or permanent failures if the correct outcome was found. As a result, we are still unsure if TSC and TCR can continue to provide favorable results in the event of a network cyber-attack.