bluuebunny/crossref_metadata_2025 huggingface dataset:
Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre
Authors: Kamuri Mohd Firdaus , Zainal Abidin Zurina , Yaacob Mohd Hanif , Hamidon Mohd Nizar , Md Yunus Nurul Amziah , Kamarudin Suryani
Published: March 2019

This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection. The prototype chip employs the pulse DEP-FFF concept to separate the cells (Escherichia coli and Saccharomyces cerevisiae) from a continuous flow, and the rate of release of the cells was measured. The separation experiments were conducted by changing the pulsing time over a pulsing time range of 2–24 s and a flow rate range of 1.2–9.6 μ L min − 1 . The frequency and voltage were set to a constant value of 1 M Hz and 14 V pk-pk, respectively. After cell sorting, the particles pass the optical fibre, and the incident light is scattered (or absorbed), thus, reducing the intensity of the transmitted light. The change in light level is measured by a spectrophotometer and recorded as an absorbance spectrum. The results revealed that, generally, the flow rate and pulsing time influenced the separation of E. coli and S. cerevisiae. It was found that E. coli had the highest rate of release, followed by S. cerevisiae. In this investigation, the developed integrated chip-in-a lab has enabled two microorganisms of different cell dielectric properties and particle size to be separated and subsequently detected using unique optical properties. Optimum separation between these two microorganisms could be obtained using a longer pulsing time of 12 s and a faster flow rate of 9.6 μ L min − 1 at a constant frequency, voltage, and a low conductivity.
Reply to “Dyslexia: Still Not a Neurodevelopmental Disorder”
Authors: Fraga González Gorka , Karipidis Iliana , Tijms Jurgen
Published: March 2019

In a recent opinion article, we explained why we think that defining developmental dyslexia as a neurodevelopmental disorder and neuroimaging studies on dyslexia are useful. A recent response has made some claims of generalized misinterpretation and misconception in the field. Since that was a direct reply to our article, we would like to clarify our opinion on some of those claims.
Interaction Narratives for Responsive Architecture
Authors: Achten Henri
Published: March 2019

In this position paper, we present the results of an ongoing theoretical investigation into the phenomenon of interactive architecture. Interaction in architecture deals with the meaningful exchange of information and physical acts between building and person. This goes beyond responsive systems like automated doors, shading systems, and so on. Most examples of interactive architecture are technological explorations that probe possibilities and the potential for interaction. In this paper we claim that this is not enough. The notion of interactive architecture is explored through social aspects, user experience, situatedness, and agent-based theory. From this we argue that interactive buildings need comprehensive and consistent styles of interaction rather than a series of isolated and unrelated interaction events. Different people in various contexts require different sets of behavior from an interactive building. These sets are conceptualized as interaction narratives, following the work of Maria Lehman. We argue that such narratives can provide a better fit of the interactive building with the user, and lead to a more profound understanding of such systems.
Adjuvant Biophysical Therapies in Osteosarcoma
Authors: Carina Valeria , Costa Viviana , Sartori Maria , Bellavia Daniele , De Luca Angela , Raimondi Lavinia , Fini Milena , Giavaresi Gianluca
Published: March 2019

Osteosarcoma (OS) is a primary bone sarcoma, manifesting as osteogenesis by malignant cells. Nowadays, patients’ quality of life has been improved, however continuing high rates of limb amputation, pulmonary metastasis and drug toxicity, remain unresolved issues. Thus, effective osteosarcoma therapies are still required. Recently, the potentialities of biophysical treatments in osteosarcoma have been evaluated and seem to offer a promising future, thanks in this field as they are less invasive. Several approaches have been investigated such as hyperthermia (HT), high intensity focused ultrasound (HIFU), low intensity pulsed ultrasound (LIPUS) and sono- and photodynamic therapies (SDT, PDT). This review aims to summarize in vitro and in vivo studies and clinical trials employing biophysical stimuli in osteosarcoma treatment. The findings underscore how the technological development of biophysical therapies might represent an adjuvant role and, in some cases, alternative role to the surgery, radio and chemotherapy treatment of OS. Among them, the most promising are HIFU and HT, which are already employed in OS patient treatment, while LIPUS/SDT and PDT seem to be particularly interesting for their low toxicity.
Reactor Selection for Effective Continuous Biocatalytic Production of Pharmaceuticals
Authors: Lindeque Rowan , Woodley John
Published: March 2019

Enzyme catalyzed reactions are rapidly becoming an invaluable tool for the synthesis of many active pharmaceutical ingredients. These reactions are commonly performed in batch, but continuous biocatalysis is gaining interest in industry because it would allow seamless integration of chemical and enzymatic reaction steps. However, because this is an emerging field, little attention has been paid towards the suitability of different reactor types for continuous biocatalytic reactions. Two types of continuous flow reactor are possible: continuous stirred tank and continuous plug-flow. These reactor types differ in a number of ways, but in this contribution, we focus on residence time distribution and how enzyme kinetics are affected by the unique mass balance of each reactor. For the first time, we present a tool to facilitate reactor selection for continuous biocatalytic production of pharmaceuticals. From this analysis, it was found that plug-flow reactors should generally be the system of choice. However, there are particular cases where they may need to be coupled with a continuous stirred tank reactor or replaced entirely by a series of continuous stirred tank reactors, which can approximate plug-flow behavior. This systematic approach should accelerate the implementation of biocatalysis for continuous pharmaceutical production.
Evaluation of Photogrammetry and Inclusion of Control Points: Significance for Infrastructure Monitoring
Authors: Oats Renee , Escobar-Wolf Rudiger , Oommen Thomas
Published: March 2019

Structure from Motion (SfM)/Photogrammetry is a powerful mapping tool in extracting three-dimensional (3D) models from photographs. This method has been applied to a range of applications, including monitoring of infrastructure systems. This technique could potentially become a substitute, or at least a complement, for costlier approaches such as laser scanning for infrastructure monitoring. This study expands on previous investigations, which utilize photogrammetry point cloud data to measure failure mode behavior of a retaining wall model, emphasizing further robust spatial testing. In this study, a comparison of two commonly used photogrammetry software packages was implemented to assess the computing performance of the method and the significance of control points in this approach. The impact of control point selection, as part of the photogrammetric modeling processes, was also evaluated. Comparisons between the two software tools reveal similar performances in capturing quantitative changes of a retaining wall structure. Results also demonstrate that increasing the number of control points above a certain number does not, necessarily, increase 3D modeling accuracies, but, in some cases, their spatial distribution can be more critical. Furthermore, errors in model reproducibility, when compared with total station measurements, were found to be spatially correlated with the arrangement of control points.
A Vibration Signal-Based Method for Fault Identification and Classification in Hydraulic Axial Piston Pumps
Authors: Casoli Paolo , Pastori Mirko , Scolari Fabio , Rundo Massimo
Published: March 2019

In recent years, the interest of industry towards condition-based maintenance, substituting traditional time-based maintenance, is growing. Indeed, condition-based maintenance can increase the system uptime with a consequent economic advantage. In this paper, a solution to detect the health state of a variable displacement axial-piston pump based on vibration signals is proposed. The pump was tested on the test bench in different operating points, both in healthy and faulty conditions, the latter obtained by assembling damaged components in the pump. The vibration signals were acquired and exploited to extract features for fault identification. After the extraction, the obtained features were reduced to decrease the computational effort and used to train different types of classifiers. The classification algorithm that presents the greater accuracy with reduced features was identified. The analysis has also showed that using the time sampling raw signal, a satisfying accuracy could be obtained, which will permit onboard implementation. Results have shown the capability of the algorithm to identify which fault occurred in the system (fault identification) for each working condition. In future works, the classification algorithm will be implemented onboard to validate its effectiveness for the online identification of the typical incipient faults in axial-piston pumps.
Unsteadiness of Tip Leakage Flow in the Detached-Eddy Simulation on a Transonic Rotor with Vortex Breakdown Phenomenon
Authors: Su Xiangyu , Ren Xiaodong , Li Xuesong , Gu Chunwei
Published: March 2019

Tip leakage vortex (TLV) in a transonic compressor rotor was investigated numerically using detached-eddy simulation (DES) method at different working conditions. Strong unsteadiness was found at the tip region, causing a considerable fluctuation in total pressure distribution and flow angle distribution above 80% span. The unsteadiness at near choke point and peak efficiency point is not obvious. DES method can resolve more detailed flow patterns than RANS (Reynolds-averaged Navier–Stokes) results, and detailed structures of the tip leakage flow were captured. A spiral-type breakdown structure of the TLV was successfully observed at the near stall point when the TLV passed through the bow shock. The breakdown of TLV contributed to the unsteadiness and the blockage effect at the tip region.
Experimental and Numerical Analysis of the Effect of Vortex Generator Height on Vortex Characteristics and Airfoil Aerodynamic Performance
Authors: Li Xinkai , Yang Ke , Wang Xiaodong
Published: March 2019

To explore the effect of the height of vortex generators (VGs) on the control effect of boundary-layer flow, the vortex characteristics of a plate and the aerodynamic characteristics of an airfoil for VGs were studied by both wind tunnel experiments and numerical methods. Firstly, the ratio of VG height (H) to boundary layer thickness (δ) was studied on a flat plate boundary layer; the values of H are 0.1δ, 0.2δ, 0.5δ, 1.0δ, 1.5δ, and 2.0δ. Results show that the concentrated vortex intensity and VG height present a logarithmic relationship, and vortex intensity is proportional to the average kinetic energy of the fluid in the height range of the VG. Secondly, the effects of height on the aerodynamic performance of airfoils were studied in a wind tunnel using three VGs with H = 0.66δ, 1.0δ, and 1.33δ. The stall angle of the airfoil with and without VGs is 18° and 8°, respectively, so the VGs increase the stall angle by 10°. The maximum lift coefficient of the airfoil with VGs increases by 48.7% compared with the airfoil without VGs, and the drag coefficient of the airfoil with VGs is 84.9% lower than that of the airfoil without VGs at an angle of attack of 18°. The maximum lift–drag ratio of the airfoil with VGs is lower than that of the airfoil without VGs, so the VGs do not affect the maximum lift–drag ratio of the airfoil. However, a VG does increase the angle of attack of the best lift–drag ratio.
Risk-Based Early Warning System for Pluvial Flash Floods: Approaches and Foundations
Authors: Hofmann Julian , Schüttrumpf Holger
Published: March 2019

In times of increasing weather extremes and expanding vulnerable cities, a significant risk to civilian security is posed by heavy rainfall induced flash floods. In contrast to river floods, pluvial flash floods can occur anytime, anywhere and vary enormously due to both terrain and climate factors. Current early warning systems (EWS) are based largely on measuring rainfall intensity or monitoring water levels, whereby the real danger due to urban torrential floods is just as insufficiently considered as the vulnerability of the physical infrastructure. For this reason, this article presents a concept for a risk-based EWS as one integral component of a multi-functional pluvial flood information system (MPFIS). Taking both the pluvial flood hazard as well as the damage potential into account, the EWS identifies the urban areas particularly affected by a forecasted heavy rainfall event and issues object-precise warnings in real-time. Further, the MPFIS performs a georeferenced documentation of occurred events as well as a systematic risk analysis, which at the same time forms the foundation of the proposed EWS. Based on a case study in the German city of Aachen and the event of 29 May 2018, the operation principle of the integrated information system is illustrated.
Behaviors and Attitudes of Polish Health Care Workers with Respect to the Hazards from Blood-Borne Pathogens: A Questionnaire-Based Study
Authors: Garus-Pakowska Anna , Górajski Mariusz
Published: March 2019

Blood-borne infections represent an important occupational health issue in health care settings. The aim of this study was to analyze behaviors of health care workers (HCWs) in the field of needlestick injuries (NSIs) as well as to learn about their attitudes to patients infected with blood-borne viruses. A total of 487 HCWs based at 26 hospitals in Poland completed an anonymous self-administered questionnaire in the period of October–December 2015. Data was analyzed using descriptive statistics and multiple logistic regression. Of the HCWs, 44.8% suffered superficial wounds, and 17.9% HCWs were cut deeply at least once. The most frequent causes of injuries were: rush (31.4%), unpredictable patient behavior (29%), and lack of attention (27%). The rate of underreporting NSIs was 45.2%. Males showed more than three times higher chance of not reporting injuries (odds ratio (OR) 3.495, 95% Confidence Interval (CI): 1.65–7.49). The nurses more often took off their protective gloves to make the procedure easier (p = 0.036). Taking off protective clothes was positively associated with long work experience (OR 1.16, 95% CI: 0.995–1.36). Recapping concerned 15.5% of doctors, 8.2% of nurses, and 11.2% of paramedics. 25.9% HCWs feared infection in the workplace, and every tenth HCW refused to help the infected patient. The longer the work experience, the greater the concern about the possibility of infection (OR 1.33, 95% CI: 0.99–1.78). Most HCWs were more cautious when dealing with an infected patient and in their opinion infected patients should be required to inform HCWs of their serological status and such information should be compulsorily transferred between different health institutions. The emphasis in the training of HCWs in the future should be on classes perfecting practical skills like paying more attention to reporting NSIs, improving occupational behaviors like avoiding needle recapping, and on the development of appropriate attitudes towards patients infected with HIV, HBV, or HCV.
Alcohol Industry CSR Organisations: What Can Their Twitter Activity Tell Us about Their Independence and Their Priorities? A Comparative Analysis
Authors: Maani Hessari Nason , van Schalkwyk May CI , Thomas Sian , Petticrew Mark
Published: March 2019

There are concerns about the accuracy of the health information provided by alcohol industry (AI)-funded organisations and about their independence. We conducted a content analysis of the health information disseminated by AI-funded organisations through Twitter, compared with non-AI-funded charities, to assess whether their messages align with industry and/or public health objectives. We compared all tweets from 2016 from Drinkaware (UK); Drinkaware.ie (Ireland); and DrinkWise (Australia), to non-AI-funded charities Alcohol Concern (UK), Alcohol Action Ireland, and FARE (Australia). Industry-funded bodies were significantly less likely to tweet about alcohol marketing, advertising and sponsorship; alcohol pricing; and physical health harms, including cancers, heart disease and pregnancy. They were significantly more likely to tweet about behavioural aspects of drinking and less likely to mention cancer risk; particularly breast cancer. These findings are consistent with previous evidence that the purpose of such bodies is the protection of the alcohol market, and of the alcohol industry’s reputation. Their messaging strongly aligns with AI corporate social responsibility goals. The focus away from health harms, particularly cancer, is also consistent with previous evidence. The evidence does not support claims by these alcohol-industry-funded bodies about their independence from industry.
Health Data Collection Before, During and After Emergencies and Disasters—The Result of the Kobe Expert Meeting
Authors: Kubo Tatsuhiko , Yanasan Alisa , Herbosa Teodoro , Buddh Nilesh , Fernando Ferdinal , Kayano Ryoma
Published: March 2019

In October 2018, the World Health Organization (WHO) convened a meeting to identify key research needs, bringing together leading experts from WHO, WHO Thematic Platform for Health Emergency and Disaster Risk Management (Health-EDRM) Research Network (TPRN), World Association for Disaster and Emergency Medicine (WADEM), the Japan International Cooperation Agency (JICA), and delegates to the Asia Pacific Conference for Disaster Medicine (APCDM) 2018. The meeting identified key research needs in five major research areas for Health-EDRM. One of the five major research areas was “Health data collection during emergency and disaster”. Experts for this research area highlighted WHO Emergency Medical Team Minimum Data Set (EMT MDS), a standardized medical data collection method during and after disasters, as an example of substantial progress, with knowledge gaps and challenges in implementation in some regions and countries (i.e., information collection methodology in medical facilities of affected local areas, seamless and practical connection between acute phase data collection and post-acute phase local surveillance). The discussion on this research area also identified key research needs in standardization of broader health-related data to inform effective Health EDRM (i.e., community vulnerabilities, hospital functional status, infrastructure, lifelines and health workforce).
The Role of Women’s Autonomy and Experience of Intimate Partner Violence as a Predictor of Maternal Healthcare Service Utilization in Nepal
Authors: Gautam Sujan , Jeong Hyoung-Sun
Published: March 2019

This study aims to identify the relationship of women’s autonomy and intimate partner violence (IPV) with maternal healthcare service utilization among married women of reproductive age in Nepal. This study used data from the 2016 Nepal Demographic and Health Survey (NDHS), which is a nationally representative sample survey. The association between outcome variables with selected factors were examined by using the Chi-square test (χ2), followed by multiple logistic regression. The sample was adjusted for multi-stage sampling design, cluster weight, and sample weight. Of the total sample, 68.4% reported attending sufficient Antenatal care (ANC) visits throughout their pregnancy, while 59.9% reported having a health facility delivery. The factors associated with both, sufficient ANC visits and institutional delivery includes ethnicity, place of residence, household wealth status, and the number of living children. Women who have access to media, and who have intended pregnancy were more likely to have sufficient ANC visits. Exposure to some forms of violence was found to be the barrier for maternal health service utilization. Attending ANC visits enables mothers to make the decision regarding skilled attendance or health facility delivery. Preventing any forms of violence need to be considered as a vital element in interventions aimed at increasing maternal health service utilization.
Visualising Combined Time Use Patterns of Children’s Activities and Their Association with Weight Status and Neighbourhood Context
Authors: Zhao Jinfeng , Mackay Lisa , Chang Kevin , Mavoa Suzanne , Stewart Tom , Ikeda Erika , Donnellan Niamh , Smith Melody
Published: March 2019

Compositional data techniques are an emerging method in physical activity research. These techniques account for the complexities of, and interrelationships between, behaviours that occur throughout a day (e.g., physical activity, sitting, and sleep). The field of health geography research is also developing rapidly. Novel spatial techniques and data visualisation approaches are increasingly being recognised for their utility in understanding health from a socio-ecological perspective. Linking compositional data approaches with geospatial datasets can yield insights into the role of environments in promoting or hindering the health implications of the daily time-use composition of behaviours. The 7-day behaviour data used in this study were derived from accelerometer data for 882 Auckland school children and linked to weight status and neighbourhood deprivation. We developed novel geospatial visualisation techniques to explore activity composition over a day and generated new insights into links between environments and child health behaviours and outcomes. Visualisation strategies that integrate compositional activities, time of day, weight status, and neighbourhood deprivation information were devised. They include a ringmap overview, small-multiple ringmaps, and individual and aggregated time–activity diagrams. Simultaneous visualisation of geospatial and compositional behaviour data can be useful for triangulating data from diverse disciplines, making sense of complex issues, and for effective knowledge translation.
Aquifer Sustainability and the Use of Desalinated Seawater for Greenhouse Irrigation in the Campo de Níjar, Southeast Spain
Authors: Aznar-Sánchez José A. , Belmonte-Ureña Luis J. , Velasco-Muñoz Juan F. , Valera Diego L.
Published: March 2019

In the Campo de Níjar (southeast Spain), an intensive horticulture model under plastic has been developed based on the use of groundwater. For many years, aquifers have been overexploited, almost generating an environmental collapse. The construction of a desalination plant was planned to improve this situation and to achieve sustainable aquifer management. However, the aquifer is still being overexploited, since farmers scarcely use desalinated seawater for irrigation. In this paper, farmers irrigating with desalinated seawater are characterized, since they contribute to aquifer sustainability. The study aimed to identify the variables which condition the use of this water resource, as well as the kinds of incentives that encourage this option. For this purpose, a survey was undertaken within a sample of 110 farmers. A cluster analysis and a binary logistic regression were employed. The results from the cluster analysis allowed the characterization of farmers who use desalinated seawater for irrigation. Furthermore, the regression model showed the variables that determine a more intensive use of this irrigation source, such as crop diversification, availability of different water resources and the conductivity of aquifer water available for irrigation. The incentives promoting the use of desalinated seawater for irrigation that most encourage farmers are the implementation of tax relief, price reductions and the obligation to install rainwater collection systems.
Prescription Infant Formulas Are Contaminated with Aluminium
Authors: Redgrove James , Rodriguez Isabel , Mahadevan-Bava Subramanian , Exley Christopher
Published: March 2019

Historical and recent data demonstrate that off-the-shelf infant formulas are heavily contaminated with aluminium. The origin of this contamination remains to be elucidated though may be imported via ingredients, packaging and processing. Specialised infant formulas exist to address health issues, such as low birth weight, allergy or intolerance and medical conditions, such as renal insufficiency. The aluminium content of these prescription infant formulas is measured here for the first time. We obtained 24 prescription infant formulas through a paediatric clinic and measured their total aluminium content by transversely heated graphite furnace atomic absorption spectrometry following microwave assisted acid/peroxide digestion. The aluminium content of ready-to-drink formulas ranged from 49.9 (33.7) to 1956.3 (111.0) μg/L. The most heavily contaminated products were those designed as nutritional supplements for infants struggling to gain weight. The aluminium content of powdered formulas ranged from 0.27 (0.04) to 3.27 (0.19) μg/g. The most heavily contaminated products tended to be those addressing allergies and intolerance. Prescription infant formulas are contaminated with aluminium. Ready-made formulas available as nutritional supplements to aid infant growth contained some of the highest concentrations of aluminium in infant formulas measured in our laboratory. However, a number of prescription infant formulas contained the lowest concentrations of aluminium yet measured in our laboratory. These higher cost specialist preparations demonstrate that the contamination of infant formulas by aluminium is not inevitable. They represent what is achievable should manufacturers wish to address the threat posed to health through infant exposure to aluminium.
Combining Mechanochemistry and Spray Congealing for New Praziquantel Pediatric Formulations in Schistosomiasis Treatment
Authors: Albertini Beatrice , Perissutti Beatrice , Bertoni Serena , Zanolla Debora , Franceschinis Erica , Voinovich Dario , Lombardo Flavio , Keiser Jennifer , Passerini Nadia
Published: March 2019

Praziquantel (PZQ) is the first line drug for the treatment of schistosome infections and is included in the WHO Model List of Essential Medicines for Children. In this study, the association of mechanochemical activation (MA) and the spray congealing (SC) technology was evaluated for developing a child-friendly PZQ dosage form, with better product handling and biopharmaceutical properties, compared to MA materials. A 1:1 by wt PZQ—Povidone coground—was prepared in a vibrational mill under cryogenic conditions, for favoring amorphization. PZQ was neat ground to obtain its polymorphic form (Form B), which has an improved solubility and bioactivity. Then, activated PZQ powders were loaded into microparticles (MPs) by the SC technology, using the self-emulsifying agent Gelucire® 50/13 as a carrier. Both, the activated powders and the corresponding loaded MPs were characterized for morphology, wettability, solubility, dissolution behavior, drug content, and drug solid state (Hot Stage Microscopy (HSM), Differential Scanning Calorimetry (DSC), X-Ray Powder Diffraction Studies (PXRD), and FT-IR). Samples were also in vitro tested for a comparison with PZQ against Schistosoma mansoni newly transformed schistosomula (NTS) and adults. MPs containing both MA systems showed a further increase of biopharmaceutical properties, compared to the milled powders, while maintaining PZQ bioactivity. MPs containing PZQ Form B represented the most promising product for designing a new PZQ formulation.
Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference
Authors: Bardi Gina T. , Al-Rayan Numan , Richie Jamaal L. , Yaddanapudi Kavitha , Hood Joshua L.
Published: March 2019

Melanoma-derived small extracellular vesicles (sEVs) participate in tumor pathogenesis. Tumor pathogenesis is highly dependent on inflammatory processes. Given the potential for melanoma sEVs to carry tumor biomarkers, we explored the hypothesis that they may contain inflammation-related mRNA content. Biophysical characterization showed that human primary melanocyte-derived sEVs trended toward being smaller and having less negative (more neutral) zeta potential than human melanoma sEVs (A-375, SKMEL-28, and C-32). Using primary melanocyte sEVs as the control population, RT-qPCR array results demonstrated similarities and differences in gene expression between melanoma sEV types. Upregulation of pro-angiogenic chemokine ligand CXCL1, CXCL2, and CXCL8 mRNAs in A-375 and SKMEL-28 melanoma sEVs was the most consistent finding. This paralleled increased production of CXCL1, CXCL2, and CXCL8 proteins by A-375 and SKMEL-28 sEV source cells. Overall, the use of primary melanocyte sEVs as a control sEV reference population facilitated the detection of inflammation-related melanoma sEV mRNA content.
CXCR2: A Novel Mediator of Mammary Tumor Bone Metastasis
Authors: Sharma Bhawna , Nannuru Kalyan C. , Saxena Sugandha , Varney Michelle L. , Singh Rakesh K.
Published: March 2019

Most breast cancer patients die due to bone metastasis. Although metastasis accounts for 5% of the breast cancer cases, it is responsible for most of the deaths. Sometimes even before the detection of a primary tumor, most of the patients have bone and lymph node metastasis. Moreover, at the time of death, breast cancer patients have the bulk of the tumor burden in their bones. Therapy options are available for the treatment of primary tumors, but there are minimal options for treating breast cancer patients who have bone metastasis. C-X-C motif chemokine receptor type 2 (CXCR2) receptor-mediated signaling has been shown to play a critical role during bone-related inflammations and its ligands C-X-C motif chemokine ligand 6 (CXCL6) and 8 (CXCL8) aid in the resorption of bone during bone metastasis. In this study, we tested the hypothesis that CXCR2 contributes to mammary tumor-induced osteolysis and bone metastasis. In the present study, we examined the role of both tumor cell-derived and host-derived CXCR2 in influencing mammary tumor cell bone metastasis. For understanding the role of tumor cell-derived CXCR2, we utilized Cl66 CXCR2 knockdown (Cl66-shCXCR2) and Cl66-Control cells (Cl66-Control) and observed a significant decrease in tumor growth and tumor-induced osteolysis in Cl66-shCXCR2 cells in comparison with the Cl66-Control cells. Next, for understanding the role of host-derived CXCR2, we utilized mice with genomic knockdown of CXCR2 (Cxcr2−/−) and injected Cl66-Luciferase (Cl66-Luc) or 4T1-Luciferase (4T1-Luc) cells. We observed decreased bone destruction and metastasis in the bone of Cxcr2−/− mice. Our data suggest the importance of both tumor cell- and host-derived CXCR2 signaling in the bone metastasis of breast cancer cells.
Characterization of Chickpea (Cicer arietinum L.) Flour Films: Effects of pH and Plasticizer Concentration
Authors: Díaz Olga , Ferreiro Tania , Rodríguez-Otero José Luis , Cobos Ángel
Published: March 2019

The use of flours as a material for biopolymer-based film preparation has gained interest due to the fact that they are a natural mixture of compatible macromolecules and due to their low cost. Chickpea flour shows a promising composition for the development of edible films. The aim of this study was to characterize and evaluate the properties of chickpea flour films as affected by pH (7 or 10) and plasticizer concentration (1% or 3% w/v) of film-forming solutions. Water vapor permeability, solubility, color, opacity, mechanical properties, thermal stability, structural changes by Fourier transform infrared analysis, and microstructure of the films were determined. Glycerol content and pH influenced chickpea flour film properties, microstructure and structural organization; interactions were also observed. The 1% glycerol films showed lower water vapor permeability, thickness, radical scavenging capacity, elongation at break and puncture deformation, and higher dry matter content, swelling, opacity, elastic modulus, and tensile and puncture strengths than 3% glycerol films. Film-forming solutions at pH 10 produced films with higher thickness and swelling, and were greener than those from solutions at neutral pH. The changes were more intense in 1% glycerol films. Glycerol concentration and pH could be combined in order to obtain chickpea flour films with different properties according to different food packaging requirements.
Towards an Efficient Data Fragmentation, Allocation, and Clustering Approach in a Distributed Environment
Authors: Abdalla Hassan , Artoli Abdel Monim
Published: March 2019

Data fragmentation and allocation has for long proven to be an efficient technique for improving the performance of distributed database systems’ (DDBSs). A crucial feature of any successful DDBS design revolves around placing an intrinsic emphasis on minimizing transmission costs (TC). This work; therefore, focuses on improving distribution performance based on transmission cost minimization. To do so, data fragmentation and allocation techniques are utilized in this work along with investigating several data replication scenarios. Moreover, site clustering is leveraged with the aim of producing a minimum possible number of highly balanced clusters. By doing so, TC is proved to be immensely reduced, as depicted in performance evaluation. DDBS performance is measured using TC objective function. An inclusive evaluation has been made in a simulated environment, and the compared results have demonstrated the superiority and efficacy of the proposed approach on reducing TC.
Analysis of an H∞ Robust Control for a Three-Phase Voltage Source Inverter
Authors: Rasool Muhammad Ahmad Usman , Khan Muhammad Mansoor , Ahmed Zahoor , Saeed Muhammad Abid
Published: March 2019

Recently, power quality improvement has gained a lot of attention due to the rapidly increasing use of power electronics equipment. Several control strategies for DC/AC Voltage Source Inverters (VSI) have been developed to obtain good quality output with low harmonic distortion. This paper proposes a robust control scheme to improve the power quality of a three-phase DC/AC VSI. The control scheme includes an outer voltage loop and an inner current loop, with both controllers designed by the standard H∞ robust control technique. The system with the proposed controller has a low total harmonic distortion (THD) and improved power quality of output voltage in the presence of linear and non-linear loads. The simulation is carried out in MATLAB/Simulink environment, and the results of the proposed control scheme are compared with the performance of dead-beat (DB) predictive control and conventional proportional integral (PI) control. It is observed from the results that the proposed control scheme outperforms other control schemes in terms of the THD level, having a better steady-state and transient performance.
Simulation-driven Selection of Electrode Materials Based on Mechanical Performance for Lithium-Ion Battery
Authors: Sarkar Abhishek , Shrotriya Pranav , Chandra Abhijit
Published: March 2019

Experimental and numerical studies have shown that mechanical loading associated with lithiation/delithiation may limit the useful life of battery electrode materials. The paper presents an approach to parameterize and compare electrode material performance based on mechanical stability. A mathematical model was developed to determine particle deformation and stress fields based upon an elastic-perfectly plastic constitutive response. Mechanical deformation was computed by combining the stress equilibrium equations with the electrochemical diffusion of lithium ions into the electrode particle. The result provided a time developing stress field which shifts from purely elastic to partially plastic deformation as the lithium-ion diffuses into the particle. The model was used to derive five merit indices that parameterize mechanical stability of electrode materials. The merit indices were used to analyze the mechanical stability for the six candidate electrode materials—three for anode materials and three for the cathode material. Finally, the paper suggests ways to improve the mechanical performance of electrode materials and identifies mechanical properties that need to be considered for selection and optimal design of electrode materials.
Prefabricated and Self-Setting Cement Laminates
Authors: Brückner Theresa , Fuchs Andreas , Wistlich Laura , Hoess Andreas , Nies Berthold , Gbureck Uwe
Published: March 2019

Polycaprolactone (PCL) fiber mats with defined pore architecture were shown to provide sufficient support for a premixed calcium phosphate cement (CPC) paste to serve as a flat and flexible composite material for the potential application in 2-dimensional, curved cranial defects. Fiber mats were fabricated by either melt electrospinning writing (MEW) or solution electrospinning (SES) with a patterned collector. While MEW processed fiber mats led to a deterioration of the cement bending strength by approximately 50%, due to a low fiber volume content in conjunction with a weak fiber-matrix interface, fiber mats obtained by solution electrospinning resulted in a mechanical reinforcement of the cement matrix in terms of both bending strength and absorbed fracture energy. This was attributed to a higher fiber volume content and a large contact area between nanosized fibers and cement matrix. Hydrophilization of the PCL scaffolds prior to lamination further improved composite strength and preserved the comparably higher fracture energy of 1.5 to 2.0 mJ/mm2. The laminate composite approach from this study was successful in demonstrating the limitations and design options of such novel composite materials. However, fiber-cement compatibility remains an issue to be addressed, since a high degree of hydrophilicity does not necessarily provoke a stronger interface.
Inverse Contrast in Non-Destructive Materials Research by Using Active Thermography
Authors: Noszczyk Paweł , Nowak Henryk
Published: March 2019

Background: it is undesirable for defects to occur in building partitions and units. There is a need to develop and improve research techniques for locating such defects, especially non-destructive techniques for active thermography. The aim of the experiment was to explore the possibility of using active thermography for testing large-sized building units (with high heat capacity) in order to locate material inclusions. Methods: as part of the experiment, two building partition models—one made of gypsum board (GB) and another made of oriented strand board (OSB)—were built. Three material inclusions (styrofoam, granite, and steel), considerably differing in their thermal parameters, were placed in each of the partitions. A 7.2 kW infrared radiator was used for thermally exciting (heating) the investigated element for 30 min. The distribution of the temperature field was studied on both sides of the partition for a few hours. Results: using the proposed investigative method, one can detect defects in building partitions under at least 22 mm of thick cladding. At a later cooling down phase, inverse temperature contrasts were found to occur—the defects, which at the beginning of cooling down were visible as warmer areas, at a later phase of cooling down are perceived as cooler areas, and vice versa (on the same front surface). In the transmission mode, the defects are always visible as areas warmer than defect-free areas. Moreover, a quantitative (defect location depth) analysis with an accuracy of up to 10% was carried out using the Echo Defect Shape method. Conclusions: active thermography can be used in construction for non-destructive materials testing. When the recording of thermograms is conducted for an appropriate length of time, inverse contrasts can be observed (on the same front surface).
Sum-Frequency Generation Spectroscopy of Plasmonic Nanomaterials: A Review
Authors: Humbert Christophe , Noblet Thomas , Dalstein Laetitia , Busson Bertrand , Barbillon Grégory
Published: March 2019

We report on the recent scientific research contribution of non-linear optics based on Sum-Frequency Generation (SFG) spectroscopy as a surface probe of the plasmonic properties of materials. In this review, we present a general introduction to the fundamentals of SFG spectroscopy, a well-established optical surface probe used in various domains of physical chemistry, when applied to plasmonic materials. The interest of using SFG spectroscopy as a complementary tool to surface-enhanced Raman spectroscopy in order to probe the surface chemistry of metallic nanoparticles is illustrated by taking advantage of the optical amplification induced by the coupling to the localized surface plasmon resonance. A short review of the first developments of SFG applications in nanomaterials is presented to span the previous emergent literature on the subject. Afterwards, the emphasis is put on the recent developments and applications of the technique over the five last years in order to illustrate that SFG spectroscopy coupled to plasmonic nanomaterials is now mature enough to be considered a promising research field of non-linear plasmonics.
Nanocrystalline Transition-Metal Gallium Oxide Spinels from Acetylacetonate Precursors via Solvothermal Synthesis
Authors: Cook Daniel S. , Kashtiban Reza J. , Krambrock Klaus , de Lima Geraldo M. , Stumpf Humberto O. , Lara Luciano R. S. , Ardisson José D. , Walton Richard I.
Published: March 2019

The synthesis of mixed-metal spinels based on substituted γ-Ga2O3 is reported using metal acetylacetonate precursors in solvothermal reactions with alcohols as solvents at 240 °C. New oxides of Cr, Mn and Fe have been produced, all of which are formed as nanocrystalline powders, as seen by high-resolution transmission electron microscopy (HR-TEM). The first chromium-gallium mixed oxide is thus formed, with composition 0.33Ga1.87Cr0.8O4 ( = vacant site). X-ray absorption near-edge spectroscopy (XANES) at the chromium K-edge shows the presence of solely octahedral Cr3+, which in turn implies a mixture of tetrahedral and octahedral Ga3+, and the material is stable on annealing to at least 850 °C. An analogous manganese material with average chemical composition close to MnGa2O4 is shown to contain octahedral Mn2+, along with some Mn3+, but a different inversion factor to materials reported by conventional solid-state synthesis in the literature, which are known to have a significant proportion of tetrahedral Mn2+. In the case of iron, higher amounts of the transition metal can be included to give an Fe:Ga ratio of 1:1. Elemental mapping using energy dispersive X-ray spectroscopy on the TEM, however, reveals inhomogeneity in the distribution of the two metals. This is consistent with variable temperature 57Fe Mössbauer spectroscopy that shows the presence of Fe2+ and Fe3+ in more than one phase in the sample. Variable temperature magnetisation and electron paramagnetic resonance (EPR) indicate the presence of superparamagnetism at room temperature in the iron-gallium oxides.
Study of TiAlN PVD Coating on Stamping Dies Used in Tinplate Food Package Production
Authors: Fernandes Liliana , Silva Francisco J. G. , Alexandre Ricardo
Published: March 2019

The food industry is increasingly demanding in terms of the quality and appearance of its product packages. The present study focuses on identifying the main wear mechanisms developed during the stamping process of these packages. During the stamping process, the presence of a tin layer on the surface of the sheet used creates difficulties in the stamping due to the transfer of material from the sheet to the tool, addition of the coefficient of friction, and premature wear of some surfaces of the tool where the contact is more pronounced. In order to understand and avoid these phenomena, a TiAlN coating deposited by a physical vapor deposition (PVD) process was used, which was studied in the laboratory to analyze the evolution of the friction force on the contact and to verify the reaction of the coating on contact with tinplate. Afterwards, the tool was coated and practical tests were performed on the stamping. The obtained results allow confirmation of a significant improvement of the wear behavior of the tool when provided with the coating and also verify that this coating presented better wear resistance than others previously tested in the same working conditions.
Spatial Mapping of Acidity and Geochemical Properties of Oxidized Tailings within the Former Eagle/Telbel Mine Site
Authors: Elghali Abdellatif , Benzaazoua Mostafa , Bussière Bruno , Genty Thomas
Published: March 2019

At some orphaned and abandoned mine sites, acid mine drainage can represent a complex challenge due to the advanced tailings’ oxidation state as well as the combination of other factors. At the field scale, several parameters control sulfides’ oxidation rates and, therefore, the acidity generation. The objective of this paper is to map the acidity and geochemical properties of oxidized tailings within a closed tailings storage facility. Based on systematic sampling, various geochemical parameters were measured within the oxidized Joutel tailings, including the: Neutralization potential, acid-generating potential, net neutralization potential, neutralization potential ratio, paste pH, thickness of oxidized, hardpan, and transition zones. The different parameters were integrated in geographical information system (GISs) databases to quantify the spatial variability of the acidity and geochemical properties of oxidized tailings. The oxidized tailings were characterized by low sulfide (mainly as pyrite) and carbonate (mainly as siderite/ankerite) contents compared to unweathered tailings. Acidic zones, identified based on paste pH, were located in the eastern portion of the southern zone and at the northern tip of the northern zone.
Photo-Reduction of CO2 by VIS Light on Polythiophene-ZSM-5 Zeolite Hybrid Photo-Catalyst
Authors: Kianička Jana , Čík Gabriel , Šeršeň František , Špánik Ivan , Sokolík Robert , Filo Juraj
Published: March 2019

A new hybrid photo-catalyst based on ZSM-5 zeolite suitable for reduction of carbon dioxide was synthesized. The photo-catalyst was prepared by oxidative polymerization of thiophene with FeCl3 in the presence of ZSM-5 with participation of ultrasound. The synthesized photo-catalyst strongly absorbs light radiation up to approx. 650 nm, with the absorption edge in the NIR region. Reactive radicals were generated by VIS light irradiation in an aqueous suspension consisting of the photo-catalyst with CO2. Formic acid and acetic acid were generated as the main products of the CO2 reduction. EPR spin trapping technique was applied to identify the reactive radical intermediates. In this work, the mechanism of product formation is also discussed.