bluuebunny/crossref_metadata_2025 huggingface dataset:
Limited yield penalties in an early transition to conservation agriculture in cotton-based cropping systems of Benin
Authors: Yemadje Pierrot Lionel , Takpa O'Neil , Amonmide Isidore , Balarabe Oumarou , Sekloka Emmanuel , Guibert Hervé , Tittonell Pablo
Published: December 2022

Transitioning toward minimum or no tillage is challenging for smallholder farmers in sub-Saharan Africa (SSA), due to the possible yield penalties during the initial years of a transition. Understanding the early impacts of such transitions is crucial in a cash crop such as cotton, on which farmers rely for their income, and is necessary to inform agroecological strategies to cope with both these challenges. This study explores the combined impact of minimum or no tillage and fertilizer regimes on agronomic parameters of cotton–cereal rotations, as practiced by smallholder farmers in Benin. A multilocation experiment was set up in three different agroclimatic zones, namely, Savalou (7°55′41″, 1°58′32″), Okpara (2°48′15″, 7°72′07″), and Soaodou (10°28′33″, 1°98′33″). In each area, the experiment was laid out as a split-plot design with four replications (main plot = soil preparation; subplot = fertilizers regimes). The treatments consisted of three different forms of soil preparation, namely, tillage, strip tillage, and no tillage or direct seeding, and four fertilization regimes, namely, basal mineral fertilizers (BMF, 200 kg ha1 of N14P18K18S6B1 + 50 kg ha1 of urea), BMF + A (200 kg ha1 of calcium phosphate amendment, 22P2O5-43CaO−4S), BMF + C (400 kg ha1 of compost), and BMF + A + C. At all sites, direct seeding led to lower below-ground biomass growth and seed cotton yields compared with conventional tillage in an early transition to conservation agriculture starting from degraded soils (2% to 25%). Weak rooting under direct seeding resulted in lower cotton yields compared with that under tillage (−12%) and strip tillage (−15%). At 45 and 90 days after emergence, cotton plants were shorter under direct seeding compared with tillage (−9% and −13%, respectively) and strip tillage (−23% and −6%, respectively). Fertilizer regimes affected seed cotton yields differently across sites and treatments, with marginal responses within soil preparation methods, but they contributed to increase yield differences between conventional and no tillage. Considering the need for sustainable practices, in the context of degraded soils and poor productivity, such limited yield penalties under CA appear to be a reasonable trade-off in the first year of a transition. Alternatively, the results from the first year of this experiment, which is meant to continue for another 5 years, suggest that strip tillage could be a sensible way to initialize a transition, without initial yield penalties, toward more sustainable soil management.
Case report: Urolithiasis, nephrolithiasis and a urinary bladder malformation in a seven-month-old alpaca cria
Authors: Schregel Johannes , Kleinschmidt Sven , von Altrock Alexandra , Hoeltig Doris , Ganter Martin , Wagener Matthias G.
Published: December 2022

Urolithiasis is a common condition in male small ruminants where predisposing factors have been identified. Occasionally, urolithiasis is diagnosed in South American camelids (SACs). However, nephrolithiasis is rarely diagnosed in ruminants. To our knowledge, this is the first report focusing on a combined appearance of nephrolithiasis and urolithiasis in an alpaca cria. A 7-month-old alpaca cria suffering from impaired urinary flow was presented for examination. On admission, the alpaca had a wet prepuce and showed a standing posture with a wide-based stance. Ultrasonographic examination of the abdomen showed a distended bladder. Clinical chemistry revealed azotemia and hypophosphatemia. After the first examination, repeated urination was observed. Conservative therapy using antibiotics, anti-inflammatory and spasmolytic drugs was started with the suspected diagnosis of urinary calculus. During the first 24 h, plasma concentrations of creatinine and urea decreased, but increased again during the following days. During the second day after admission, urination was not observed for 16 h while the concentration of urea and creatinine further increased. Therefore, the animal was euthanized due to financial concerns of the owner. Necropsy revealed that calculi were located in the left kidney as well as in the urethra. In addition, the animal exhibited uroperitoneum. The urinary bladder was intact, moderately distended with urine and showed a malformation, which was covered with a translucent mucosal membrane. Histologic examination revealed that this malformation was a bladder diverticulum. The extent to which the unilateral nephroliths affected the general condition and renal function of the animal is unclear, since the uroliths also cause azotemia, and abdominal pain. Further studies are needed for a better understanding of obstructive urinary disease in SACs.
The Impact of Islamic Banking Corporate Governance on Green Banking
Authors: Sehen Issa Jabbar , Abbaszadeh Mohammad Reza , Salehi Mahdi
Published: December 2022

The primary purpose of the research is to investigate the impact of Islamic banking corporate governance on green banking in Iraq. In other words, the current study seeks to find an answer to whether corporate governance in Islamic banking can affect green banking in Iraq. For this purpose, the research method is applied based on the objective and descriptive survey. The statistical population of this research is all the managers, employees, and customers of the public and private banks of Iraq, and a total of 70 questionnaires have been completed and analyzed. The sampling method is non-random, and the available population was selected as the sample size. In this research, PLS tests have been used to investigate the effect of independent variables on the dependent variable. The results indicate that corporate governance in Islamic banking has had a positive effect on green banking, meaning that the increase of corporate governance mechanisms in Iraqi Islamic banking increase the level of attention of Iraqi Islamic banks to green banking matters. The current research was conducted in Iraq’s developing or emerging financial markets, which are highly competitive and under insufficient supervision.
Intellectual Capital of Technology-Based Incubators
Authors: Rodrigues M. Carolina Martins , Barbosa Raul Pommer , Barbieri da Rosa Luciana Aparecida , Sousa Maria José , Zavatti Campos Waleska Yone Yamakawa
Published: December 2022

The objective of this work is to evaluate the associations between the intellectual capital of technology-based incubators in the sustainability of incubated companies located in Portugal. For this purpose, the methodological strategy employed was the survey, and to test the hypotheses the Partial Least Squares Structural Equation Modeling PLS-SEM method was applied from a sample of 82 incubated company managers. The results show that the intellectual capital of the incubator company has a direct and positive relationship with the innovative capacity, satisfaction, and sustainability of the incubated company. In turn, the incubated company’s innovative capacity has a direct and positive impact on sustainability itself. In addition, both the sustainability of the incubated company and its levels of satisfaction with the incubated company has a positive and direct impact on its competitive success. The management implications include the perception that the greater the effort to improve the human capital, structural capital, and relational capital of the incubated companies, the better will be the results achieved in supporting companies, helping start-ups develop sustainably and competitively in the market.
Overexpressing OsPYL/RCAR7 Improves Drought Tolerance of Maize Seedlings by Reducing Stomatal Conductance
Authors: Hong Joon Ki , Lee Yeon-Hee , Kim Beom-Gi , Lee Gang Seob , Jang Hee Jeung , Song Giha , Suh Eun Jung , Park Sang Ryeol
Published: December 2022

Drought stress is a serious abiotic factor limiting the quality and yield of maize (Zea mays). To produce maize plants with enhanced drought tolerance, we generated transgenic maize plants overexpressing OsPYL/RCAR7, encoding an abscisic acid receptor. We crossed the selected lines with maize variety B73 and obtained F1 hybrid seeds. Initial screening suggested that the transgenic lines were more drought tolerant than wild-type plants. Analysis using the DroughtSpotter platform indicated that expressing OsPYL/RCAR7 enhanced drought resistance in transgenic maize seedlings by reducing water loss. In addition, the stomatal conductance of the leaf surface was 30% lower in OsPYL/RCAR7-overexpressing plants than in wild-type ones. After drought treatment, OsPYL/RCAR7-overexpressing maize showed a much higher survival rate than the wild type, suggesting that expressing OsPYL/RCAR7 reduced the negative effects of drought exposure on stomatal conductance and enhanced water use efficiency. Furthermore, the expression levels of drought-tolerance–related abscisic acid–signaling genes ABP2 and RAB16A were higher in the transgenic plants than in the wild type. Taken together, our data indicate that the seedlings of transgenic maize expressing the gene OsPYL/RCAR7 showed increased tolerance to drought stress, raising the possibility that stress-related genes from monocotyledonous crops could be used as genetic resources to improve the agricultural traits of maize.
Prospects for Increasing the Efficacy of Plant Resistance Inducers Stimulating Salicylic Acid
Authors: Urban Laurent , Lauri Félicie , Ben Hdech Douae , Aarrouf Jawad
Published: December 2022

Systemic acquired resistance is a powerful mechanism, based on the salicylic acid (SA) signaling pathway, which allows plants to resist to a wide range of pathogens. High SA, moreover, plays a key role in plant tolerance to abiotic stress. It seems, therefore, desirable to supply analogs of SA or stimulate the production of endogenous SA. Unfortunately, the chemical substances or physical means used for this effect often display a variable efficacy. After providing a review of them, we defend three major ideas: (i) plant resistance inducers (PRIs) must be combined for higher efficacy, notably for exploiting synergic effects between the SA and other signaling pathways, (ii) disease pressure can be reduced by exploiting the fungicidal properties displayed by some PRIs, (iii) biostimulants and crop management techniques should be used to ensure that plants have the resources they need to synthesize the compounds and structures required for efficient and lasting resistance. Some PRIs could also be used for their biostimulant effects in stress conditions. It could be concluded that holistic approaches which jointly address the issues of defense and tolerance stimulation, disease pressure and resource availability in plants are the ones that will allow for substantial reduction in fungicide use without sacrificing crop performance.
Confirmation of the Mechanisms of Resistance to ACCase-Inhibiting Herbicides in Chinese Sprangletop (Leptochloa chinensis (L.) Nees) from South Sulawesi, Indonesia
Authors: Kurniadie Denny , Widianto Ryan , Aprilia Annisa Nadiah , Damayanti Farida
Published: December 2022

Chinese sprangletop (Leptochloa chinensis (L.) Nees) is recognized as the most disturbing weed in rice fields in Africa, Australia, and Asia due to causing a significant reduction in yields. The habit of most lowland rice farmers in Indonesia is using high doses of herbicides more than once without any rotation, leading to increased weed resistance potential. Therefore, this study aimed to confirm the resistance level of L. chinensis to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides using the whole-plant pot test method. We identified other herbicides that can control the resistant biotype and performed DNA sequencing on a sample to determine mutations present in the biotype′s ACCase gene through polymerase chain reaction. The herbicide dose–response experiment showed that the L. chinensis from Bantimurung Subdistrict, Maros Regency, South Sulawesi, exhibited resistance to ACCase inhibitors (metamifop and cyhalofop-butyl), which is the first case of resistance to ACCase herbicides reported in Indonesia. An acetolactate synthase inhibitor (bispyribac-sodium) and a 1-deoxy-d-xylulose-5-phosphate synthase (DOXP) inhibitor (clomazone) were effective at controlling the resistant biotypes of L. chinensis, so could considered for use in rotation or as an ingredient in mixed herbicides. Single-nucleotide substitution of guanine for thiamine at position 6081 (TGG; susceptible, TGT; Maros) that causes a Trp2027Cys mutation in the target gene contributes to the resistance of the Maros biotype to ACCase inhibitors.
Remediation of Pasture Dieback Using Plant Growth Promotant
Authors: Whitton Maria M. , Ren Xipeng , Yu Sung J. , Trotter Tieneke , Stanley Dragana , Bajagai Yadav S.
Published: December 2022

Pasture dieback is a syndrome of unknown cause affecting grasses in Australia, creating significant economic losses to farmers by reducing available livestock feed and paddock carrying capacity. RC3 is a commercial plant growth stimulant tri-sodium salt of trimercapto-S-triazine (TMT) and potassium humate as active ingredients. TMT is commonly used for soil and wastewater remediation by capturing and binding heavy metals, while potassium humate is an organic compound used as a plant growth promoter. We investigated the ability of RC3 to restore soil health and productivity under pasture dieback conditions. RC3 was applied on pasture dieback affected paddock replicate plots once, at a rate of 4 mL/m2, and soil core samples were taken weekly to analyse microbial communities. Plants were collected regularly to measure dry matter and plant morphometrics. Twenty weeks after a single application, dry matter increased in RC3 plots by 900 kg/ha compared to control plots, and at week 48, eleven months after the single application, RC3 plots showed a trend of more grass and dicot species than the control. Morphometric measures suggest minor improvements in dicotyledon plants. Alpha diversity did not change with the application of RC3. Temporal correlation analysis shows that RC3 steadily reduced the presence of genera predominant in poor soils and with extreme environmental conditions over time and prevented the decline of beneficial genera, such as Marmoricola, Actinomadura, Dactylosporangium, and mle1-7.
Efficient Separating Collected Seedlings Based on Precise Subdivision Air Streams
Authors: Jiang Delong , Xia Hongmei , Yang Yi , Duan Zhiyong , Gu Meizhang , Chu Qi , Yang Yanli , Gu Song
Published: December 2022

Automatic grafting and cutting machines can be employed to satisfy the increasing demand for seedlings without soil-borne diseases in a short period. The main approach used to feed seedlings for automatic grafting and cutting machines is artificial, which limits the improvement of grafting and cutting machine productivity. The separation system with a subdivision air stream can stably feed seedlings for cutting and grafting machines; however, the separation efficiency is low when a few seedlings are in the separator. To solve this problem, a feedback monitoring device with a photosensitive sensor as the sensing element and feedback functions on the status of seedlings in the separator was developed. Through experiments using a photosensitive sensor to monitor the separation process of tomato seedlings, the results showed that the effect on the effective seedling blowing rate varied from large to small depending on the seedling size, light intensity, and sensor diameter. The results of separation experiment showed that the productivity of the entire system was 8784 plants/h, which satisfies the productivity needs of the grafting and cutting machine. Compared with the separation device without feedback monitoring, the productivity increased by 39%, the damage rate decreased by 4%, and the number of subdivided air stream operations was reduced by 47%.
Different Suitability of Olive Cultivars Resistant to Xylella fastidiosa to the Super-Intensive Planting System
Authors: Camposeo Salvatore , Stellacci Anna Maria , Romero Trigueros Cristina , Alhajj Ali Salem , Vivaldi Gaetano Alessandro
Published: December 2022

Until today, only Leccino and Fs-17 (=Favolosa®) olive cultivars proved resistant to Xylella fastidiosa subsp. pauca (Xfp) due to a low presence of bacteria in the xylem. Integrated disease management in olive growing areas threatened by the spread of Xfp is crucial to overcoming the environmental, economic and social crisis. Since the EU Decision allows for the plantation of resistant olive cultivars in infected areas, there is a need to define a suitable plantation system for these cultivars. The adoption of new planting systems, such as intensive and super-intensive (SHD), could compensate for the economic losses and restore the olive agroecosystem. The aim is to ascertain the suitability of the available Xfp-resistant cultivars to SHD planting systems that demonstrate the best economic and environmental sustainability. Hence, a five-year study was established in an experimental SHD olive orchard (Southern Italy) in order to analyse the main vegetative and productive traits of Leccino and Fs-17, together with four other Italian cultivars (Cipressino, Coratina, Frantoio and Urano), compared with the well-adapted cultivars to SHD orchards (Arbequina and Arbosana), by means of the von Bertalanffy function. The results indicated that cv. Fs-17 showed sufficient suitability for SHD planting systems, giving the best-accumulated yield despite some canopy growth limitations, whereas cv. Leccino did not show satisfactory results in terms of both vegetative and yield parameters, confirming its suitability for intensive planting systems. These results are useful for optimizing integrated resistance management in Xfp-infected areas by planting resistant host plants.
Prevalence and Prognosis of Atenolol-Responsive Systolic Anterior Motion of the Septal Mitral Valve Leaflet in Young Cats with Severe Dynamic Left Ventricular Outflow Tract Obstruction
Authors: Kortas Michelle , Szatmári Viktor
Published: December 2022

Background: Severe dynamic left ventricular outflow tract obstruction (DLVOTO) secondary to the systolic anterior motion of the septal mitral valve leaflet (SAM) can result either from congenital mitral valve disorders or left ventricular concentric hypertrophy of any cause, in cats commonly hypertrophic cardiomyopathy (HCM). Though HCM cannot be reversed, the question remains whether atenolol can cure cats with severe DLVOTO resulting from a presumed mitral valve dysplasia. Methods: In this retrospective case series, client-owned asymptomatic cats younger than 1.5 years with echocardiographic evidence of SAM and severe DLVOTO were included. Oral atenolol therapy and recheck echocardiography after 2–3 months were recommended. The owners and referring veterinarians were called for long-term follow-up information. Results: Of the 28 included cats, 23 were treated with atenolol. Recheck echocardiography performed in 17 cats showed a resolution of SAM in 47%. In the long term, SAM remained absent in only 9% of the treated cats. Cardiac-related death occurred in 26% of the atenolol-treated cats. Conclusions: The long term benefit of twice-daily atenolol therapy was documented in 9% of cats. Whether the cats where atenolol failed to resolve DLVOTO on long-term had HCM, or a therapy-resistant congenital primary mitral valve disorder remains unclear.
Prevalence and Molecular Characteristics of Bovine Respiratory Syncytial Virus in Beef Cattle in China
Authors: Chang Yiming , Yue Hua , Tang Cheng
Published: December 2022

Bovine respiratory syncytial virus (BRSV) is an important pathogen of the bovine respiratory disease complex (BRDC); however, its prevalence and molecular characteristics in China remain largely unknown. In this study, 788 nasal swabs from 51 beef cattle farms with BRDC outbreaks in 16 provinces and one municipality were collected from October 2020 to July 2022, and 18.65% (147/788) of samples from 23 farms across 11 provinces were detected as BRSV-positive by reverse transcription-insulated isothermal PCR (RT-iiPCR) assay. Further, 18 complete G gene sequences were classified into BRSV subgroup III, and 25 complete F gene sequences were obtained from 8 and 10 provinces. Compared to the known BRSV strains in GenBank, the G proteins and F proteins in this study shared several identical amino acid (aa) mutations. Moreover, five nearly complete genome sequences were obtained and clustered into a large branch with two America BRSV subgroup III strains (KU159366 and OM328114) rather than the sole Chinese strain (MT861050) but were located in an independent small branch. In conclusion, this study reveals that BRSV has a wide geographical distribution in China, and subgroup III strains, which have unique evolution characteristics, are the dominant strains. The results contribute to a better understanding of the prevalence and genetic evolution of BRSV.
Evaluation of Garlic Juice Processing Waste Supplementation in Juvenile Black Rockfish (Sebastes schlegelii) Diets on Growth Performance, Antioxidant and Digestive Enzyme Activity, Growth- and Antioxidant-Related Gene Expression, and Disease Resistance against Streptococcus iniae
Authors: Oh Hwa Yong , Lee Tae Hoon , Lee Da-Yeon , Lee Chang-Hwan , Sohn Min-Young , Kwon Ryeong-Won , Kim Jeong-Gyun , Kim Hee Sung , Kim Kyoung-Duck
Published: December 2022

An 8-week feeding trial was conducted to evaluate the effects of various dietary levels of garlic juice processing waste (GJPW) on the growth, feed utilization, digestive and antioxidant enzyme activity, growth- and antioxidant-related gene expression, and resistance to Streptococcus iniae infection of juvenile black rockfish (Sebastes schlegelii). A total of 450 juvenile rockfish were randomly distributed into 30 L rectangular tanks (30 fish per tank). Five experimental diets were prepared in triplicate. The fish were fed experimental diets supplemented with GJPW at concentrations of 0 (GJPW0, control), 2.5 (GJPW2.5), 5 (GJPW5), 7.5 (GJPW7.5), and 10 g kg−1 (GJPW10) diet. All of the GJPW-supplemented treatments (2.5, 5, 7.5, and 10 g kg−1) significantly enhanced weight gain (WG), specific growth rate (SGR), feed efficiency (FE), protein efficiency ratio (PER), and digestive enzyme activity (amylase, trypsin, and lipase). A decreasing trend was seen in plasma aspartate aminotransferase (ALT), alanine aminotransferase (AST), and glucose (GLU) content with increasing dietary levels of GJPW. In contrast, plasma lysozyme and antioxidant enzyme activities were significantly increased with increasing dietary GJPW levels. Furthermore, GJPW administration significantly upregulated the expression of insulin-like growth factor-1 (IGF-1), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) in the liver of rockfish. A challenge test with S. iniae showed significantly higher resistance in the GJPW-supplemented treatments than in the control. In short, dietary supplementation GJPW enhanced growth performance and antioxidant response in juvenile black rockfish, with suitable effects in fish fed with 2.5 g kg−1 GJPW for 8 weeks.
Effects of Energy and Protein Levels on Laying Performance, Egg Quality, Blood Parameters, Blood Biochemistry, and Apparent Total Tract Digestibility on Laying Hens in an Aviary System
Authors: Kim Chan-Ho , Kang Hwan-Ku
Published: December 2022

This study was performed to investigate the effects of apparent metabolizable energy (AMEn) and protein levels on laying performance, egg quality, blood parameters, blood biochemistry, and apparent total tract digestibility of energy and nutrients in diets fed to laying hens in an aviary system. A total of 560 Hy-Line Brown laying hens (age = 30 week) were distributed in a completely randomized experimental design in 2 × 2 factorial arrangements with 2 metabolizable energy levels (2700 and 2800 kcal AMEn/kg) and 2 protein levels (16.5 and 14.5% CP). Four treatments and four replicates of 40 birds each (stocking density = 15 birds/m2) were prepared. Results revealed no significant interaction between AMEn and CP in the diet in terms of egg production, floor eggs, broken and dirty egg production, egg mass, feed intake, and feed conversion ratio of laying hens. However, egg weight was affected. As dietary energy and CP levels (2800 kcal of AMEn/kg and 16.5% CP) increased, egg weight increased (p < 0.05). Egg weight, feed intake, and feed conversion ratio significantly differed (p < 0.05) as the energy content in the feed increased. Ether extract significantly varied (p < 0.05) as the energy content in the feed increased. In conclusion, laying performance and egg quality of Hy-Line Brown laying hens in the middle stage of egg production (30 to 50 weeks) were not affected by different dietary energy and protein levels, but feed intake decreased with an increasing level of AMEn in diets. Ether extract significantly varied as the energy content in the feed increased.
Garrano Horses Perceive Letters of the Alphabet on a Touchscreen System: A Pilot Study
Authors: Schubert Clara-Lynn , Ryckewaert Barbara , Pereira Carlos , Matsuzawa Tetsuro
Published: December 2022

This study aimed to use a computer-controlled touchscreen system to examine visual discrimination in Garrano horses (Equus caballus), an endangered breed of pony belonging to the Iberian horse family. This pilot study focused on the perceptual similarity among letters of the alphabet. We tested five horses in a one-male unit (OMU) living permanently in a semi-free enclosure near their natural habitat in Serra d’Arga, northern Portugal. Horses were trained to nose-touch black circles that appeared on the screen. Then, they were tested for discrimination of five letters of the Latin alphabet in Arial font, namely O, B, V, Z, and X, using a two-choice discrimination task. The confusion matrix of letter pairs was used to show the MDS and to identify the relative contribution of shape features. The results showed perceptual similarities among letters with curvatures pitted against those of straight lines. Shape perception in horses seems to share features with that of humans and other animals living in different niches. The touchscreen system proved to be an objective and innovative way of studying cognition in the socially organized group of horses. The automated system can promote the welfare of captive horses by maximizing their freedom of movement.
Effects of Castration Age on the Growth Performance of Nubian Crossbred Male Goats
Authors: Chen Yu-An , Chen Jing-Yan , Chen Wei-Qun , Wang Wen-Yen , Wu Hsi-Hsun
Published: December 2022

To determine the optimal timing for performing castration on goats, eighteen male Nubian crossbred goats were randomly assigned to two groups and castrated at 3 months and 6 months of age, respectively. Daily dry matter intake, biweekly body weights, and ultrasonic measurements of longissimus dorsi muscle growth were recorded. Results indicated that there was no significant difference between the two groups in terms of the blood parameter analysis (except testosterone, 0.36 ± 0.26 vs. 3.61 ± 0.27 ng/mL at 25 weeks old), economic analysis, and growth performance, including final body weight, total weight gain, average daily gain, total dry-matter intake, and feed conversion ratio (p > 0.05). However, the longissimus dorsi muscle depth of goats castrated at 6 months of age was significantly higher than that of goats castrated at 3 months of age. In conclusion, castration timing does not have a significant effect on the growth performance of goats; therefore, castrating goats at 3 months of age may be the best practice considering animal welfare and possible risks associated with late castration.
State of the Art and Future Prospects of Virtual and Augmented Reality in Veterinary Medicine: A Systematic Review
Authors: Aghapour Masoud , Bockstahler Barbara
Published: December 2022

Virtual reality and augmented reality are new but rapidly expanding topics in medicine. In virtual reality, users are immersed in a three-dimensional environment, whereas in augmented reality, computer-generated images are superimposed on the real world. Despite advances in human medicine, the number of published articles in veterinary medicine is low. These cutting-edge technologies can be used in combination with existing methods in veterinary medicine to achieve diagnostic/therapeutic and educational goals. The purpose of our review was to evaluate studies for their use of virtual reality and augmented reality in veterinary medicine, as well as human medicine with animal trials, to report results and the state of the art. We collected all of the articles we included in our review by screening the Scopus, PubMed, and Web of Science databases. Of the 24 included studies, 11 and 13 articles belonged to virtual reality and augmented reality, respectively. Based on these articles, we determined that using these technologies has a positive impact on the scientific output of students and residents, can reduce training costs, and can be used in training/educational programs. Furthermore, using these tools can promote ethical standards. We reported the absence of standard operation protocols and equipment costs as study limitations.
SARS-CoV-2 Virus-like Particles (VLPs) Specifically Detect Humoral Immune Reactions in an ELISA-Based Platform
Authors: Hirschberg Stefan , Bauer Hannes , Kamhieh-Milz Julian , Ringel Frauke , Harms Christoph , Eddin Omar Kamal , Pruß Axel , Hanack Katja , Schulze-Forster Kai
Published: December 2022

A key in controlling the SARS-CoV-2 pandemic is the assessment of the immune status of the population. We explored the utility of SARS-CoV-2 virus-like particles (VLPs) as antigens to detect specific humoral immune reactions in an enzyme-linked immunosorbent assay (ELISA). For this purpose, SARS-CoV-2 VLPs were produced from an engineered cell line and characterized by Western blot, ELISA, and nanoparticle tracking analysis. Subsequently, we collected 42 serum samples from before the pandemic (2014), 89 samples from healthy subjects, and 38 samples from vaccinated subjects. Seventeen samples were collected less than three weeks after infection, and forty-four samples more than three weeks after infection. All serum samples were characterized for their reactivity with VLPs and the SARS-CoV-2 N- and S-protein. Finally, we compared the performance of the VLP-based ELISA with a certified in vitro diagnostic device (IVD). In the applied set of samples, we determined a sensitivity of 95.5% and a specificity of 100% for the certified IVD. There were seven samples with an uncertain outcome. Our VLP-ELISA demonstrated a superior performance, with a sensitivity of 97.5%, a specificity of 100%, and only three uncertain outcomes. This result warrants further research to develop a certified IVD based on SARS-CoV-2 VLPs as an antigen.
Study of Prescription-Indication of Outpatient Systemic Anti-Fungals in a Colombian Population. A Cross-Sectional Study
Authors: Valladales-Restrepo Luis Fernando , Ospina-Cano Juan Alberto , Aristizábal-Carmona Brayan Stiven , López-Caicedo Diana Fiorella , Toro-Londoño Melissa , Gaviria-Mendoza Andrés , Machado-Duque Manuel Enrique , Machado-Alba Jorge Enrique
Published: December 2022

The inappropriate use of antifungals is associated with greater antimicrobial resistance, costs, adverse events, and worse clinical outcomes. The aim of this study was to determine prescription patterns and approved and unapproved indications for systemic antifungals in a group of patients in Colombia. This was a cross-sectional study on indications for the use of systemic antifungals in outpatients from a drug dispensing database of approximately 9.2 million people affiliated with the Colombian Health System. Sociodemographic, pharmacological, and clinical variables were considered. Descriptive, bivariate, and multivariate analyses were performed. A total of 74,603 patients with antifungal prescriptions were identified; they had a median age of 36.0 years (interquartile range: 22.0–53.0 years), and 67.3% of patients were women. Fluconazole (66.5%) was the most prescribed antifungal for indications such as vaginitis, vulvitis, and vulvovaginitis (35.0%). A total of 29.3% of the prescriptions were used in unapproved indications. A total of 96.3% of ketoconazole users used the medication in unapproved indications. Men (OR: 1.91; CI95%: 1.79–2.04), <18 years of age (OR: 1.20; CI95%: 1.11–1.31), from the Caribbean region (OR: 1.26; CI95%: 1.18–1.34), with chronic obstructive pulmonary disease (OR: 1.80; CI95%: 1.27–2.54), prescriptions made by a general practitioner (OR: 1.17; CI95%: 1.04–1.31), receiving comedications (OR: 1.58; CI95%: 1.48–1.69), and the concomitant use of other antimicrobials (OR: 1.77; CI95%: 1.66–1.88) were associated with a higher probability that the antifungal was used for unapproved indications; deep mycosis (OR: 0.49; CI95%: 0.41–0.58), prescribing fluconazole (OR: 0.06; CI95%: 0.06–0.06), and having diabetes mellitus (OR: 0.33; CI95%: 0.29–0.37), cancer (OR: 0.13; CI95%: 0.11–0.16), or HIV (OR: 0.07; CI95%: 0.04–0.09) reduced this risk. Systemic antifungals were mostly used for the management of superficial mycoses, especially at the gynecological level. In addition, more than a quarter of patients received these medications in unapproved indications, and there was broad inappropriate use of ketoconazole.
The Role of Heme Oxygenase-1 as an Immunomodulator in Kidney Disease
Authors: Athanassiadou Virginia , Plavoukou Stella , Grapsa Eirini , Detsika Maria G.
Published: December 2022

The protein heme oxygenase (HO)-1 has been implicated in the regulations of multiple immunological processes. It is well known that kidney injury is affected by immune mechanisms and that various kidney-disease forms may be a result of autoimmune disease. The current study describes in detail the role of HO-1 in kidney disease and provides the most recent observations of the effect of HO-1 on immune pathways and responses both in animal models of immune-mediated disease forms and in patient studies.
Towards Domain-Specific Knowledge Graph Construction for Flight Control Aided Maintenance
Authors: Li Chuanyou , Yang Xinhang , Luo Shance , Song Mingzhe , Li Wei
Published: December 2022

Flight control is a key system of modern aircraft. During each flight, pilots use flight control to control the forces of flight and also the aircraft’s direction and attitude. Whether flight control can work properly is closely related to safety such that daily maintenance is an essential task of airlines. Flight control maintenance heavily relies on expert knowledge. To facilitate knowledge achievement, aircraft manufacturers and airlines normally provide structural manuals for consulting. On the other hand, computer-aided maintenance systems are adopted for improving daily maintenance efficiency. However, we find that grass-roots engineers of airlines still inevitably consult unstructured technical manuals from time to time, for example, when meeting an unusual problem or an unfamiliar type of aircraft. Achieving effective knowledge from unstructured data is inefficient and inconvenient. Aiming at the problem, we propose a knowledge-graph-based maintenance prototype system as a complementary solution. The knowledge graph we built is dedicated for unstructured manuals referring to flight control. We first build ontology to represent key concepts and relation types and then perform entity-relation extraction adopting a pipeline paradigm with natural language processing techniques. To fully utilize domain-specific features, we present a hybrid method consisting of dedicated rules and a machine learning model for entity recognition. As for relation extraction, we leverage a two-stage Bi-LSTM (bi-directional long short-term memory networks) based method to improve the extraction precision by solving a sample imbalanced problem. We conduct comprehensive experiments to study the technical feasibility on real manuals from airlines. The average precision of entity recognition reaches 85%, and the average precision of relation extraction comes to 61%. Finally, we design a flight control maintenance prototype system based on the knowledge graph constructed and a graph database Neo4j. The prototype system takes alarm messages represented in natural language as the input and returns maintenance suggestions to serve grass-roots engineers.
Research on the Effective Reduction of Accidents on Operating Vehicles with fsQCA Method—Case Studies
Authors: Zhang Xue , Lu Yi , Huang Xianwen , Zhou Aizhao
Published: December 2022

Traffic accidents are caused by man mainly, especially improper driving. The effective way to reduce safety accidents caused by improper driving is to find out the cause and path causing the accident, block the accident formation chain, and then reduce safety accidents. Therefore, using data from 337 road transport safety accidents in operating vehicles caused by improper driving behavior as the initial research sample, this paper uses the fuzzy set qualitative comparative analysis method to conduct a group analysis of typical cases and identifies the cause and path of safety accidents. The research results show that there are mainly four types of paths leading to safety accidents. According to the distribution of their core conditions, safety accidents are highly correlated with passenger transport and the degree of individualization of business models on operating vehicles. The following measures can be taken to prevent safety accidents: strengthen the supervision of operating enterprises (especially individual operations and individual-affiliated operations), carry out detailed safety training, and fully use advanced technology such as big data and high-tech means. The research results will help traffic and road transport management departments to prevent road safety accidents more effectively, which is of great significance to promoting the healthy development of road transport.
Anti-Breast Cancer Activity of Essential Oil: A Systematic Review
Authors: Mustapa Mohammad Adam , Guswenrivo Ikhsan , Zuhrotun Ade , Ikram Nur Kusaira Khairul , Muchtaridi Muchtaridi
Published: December 2022

Breast cancer is the second highest cancer-related death worldwide. The treatment for breast cancer is via chemotherapy; however, occurrences of multidrug resistance, unselective targets, and physicochemical problems suggest that chemotherapy treatment is ineffective. Therefore, there is a need to find better alternatives. Essential oil is a plant secondary metabolite having promising bioactivities and pharmacological effects, including anti-breast cancer capabilities. This review intends to discuss and summarize the effect of essential oils on anti-breast cancer from published journals using keywords in PubMed, Scopus, and Google Scholar databases. Our findings reveal that the compositions of essential oils, mainly terpenoids, have excellent anti-breast cancer pharmacological effects with an IC50 value of 0.195 μg/mL. Hence, essential oils have potential as anti-breast cancer drugs candidates with the highest efficacy and the fewest side effects.
A Digital Denoising Method Based on Data Frequency Statistical Filtering
Authors: Li Zhongshen , Luo Tao , Lv Yuan , Guo Tong , Lin Tianliang
Published: December 2022

Noise amplitude in original time domain data is usually discrete and sparse. This article presents a digital filter denoising method based on statistical frequencies of the signal values. The effective signal and noise signal are identified by comparing the frequency of the value of each pixelin the original signal with the preset validity discrimination threshold. Signals recognized as valid will be output directly, while noise signals will be replaced by the mean value of their surrounding pixel values. Compared to classical digital filtering methods such as mean filtering and median filtering, this method may improve signal recognition accuracy and has the potential to remove random noise while retaining details. An image noise reduction software based on frequency statistics was developed in the MATLAB environment. Noise reduction based on this algorithm was implemented on a portrait image with a noise density of 5%~40%, and noise reduction efficiency was compared to the classical noise reduction algorithms. The experimental results show that the PSNR of the proposed new method exceeds 41, reaching the same level as switching median filtering and adaptive filtering and preceding mean filtering. The SSIM of the new method exceeds 0.97, which is better than other classical methods. Additionally, the higher the noise density, the more obvious the advantage of this method.
Application of Digital Twin in Handling and Transportation of Hazardous Chemicals
Authors: Li Xiao , Zhang Yi , Li Chaoyang , Wang Tao , Xi Changqin
Published: December 2022

In China, the Ministry of Transport stressed the need to “Strengthen the application of active safety technology”. The transportation of hazardous chemicals represented by LNG, LPG, and liquefied hydrocarbons is the weak link in traffic safety. The aim of this study is to apply digital twin (hereinafter referred to as DT) technology to the whole process of handling (including loading and unloading) and transportation of hazardous chemicals to help improve the anti-risk ability of road networks at all levels. The method is intended to design a monitoring system covering operation visualization, information fusion, cargo tracking, and hazard source monitoring that is based on DT technology and multi-source data acquisition technology. First, DT technology in the areas of hazardous chemicals handling and transportation is discussed. Then, the DT system is designed, including the system construction, functions, and the means of achieving these functions. Finally, taking the procedure in LNG road transportation as an example, we illustrate the application of DT in its four stages. This system is used to present the evolutionary path of accidents that occur in different links and assist in testing the rationality of the comprehensive disposal plan.
On the Use of a Hydrogen-Fueled Engine in a Hybrid Electric Vehicle
Authors: Beccari Stefano
Published: December 2022

Hybrid electric vehicles are currently one of the most effective ways to increase the efficiency and reduce the pollutant emissions of internal combustion engines. Green hydrogen, produced with renewable energies, is an excellent alternative to fossil fuels in order to drastically reduce engine pollutant emissions. In this work, the author proposes the implementation of a hydrogen-fueled engine in a hybrid vehicle; the investigated hybrid powertrain is the power-split type in which the engine, two electric motor/generators and the drive shaft are coupled together by a planetary gear set; this arrangement allows the engine to operate independently from the wheels and, thus, to exploit the best efficiency operating points. A set of numeric simulations were performed in order to compare the gasoline-fueled engine with the hydrogen-fueled one in terms of the thermal efficiency and total energy consumed during a driving cycle. The simulation results show a mean engine efficiency increase of around 17% when fueled with hydrogen with respect to gasoline and an energy consumption reduction of around 15% in a driving cycle.
Quantitative Analysis of Retinal Vascular Leakage in Retinal Vasculitis Using Machine Learning
Authors: Keino Hiroshi , Wakitani Tomoki , Sunayama Wataru , Hatanaka Yuji
Published: December 2022

Retinal vascular leakage is known to be an important biomarker to monitor the disease activity of uveitis. Although fluorescein angiography (FA) is a gold standard for the diagnosis and assessment of the disease activity of uveitis, the evaluation of FA findings, especially retinal vascular leakage, remains subjective and descriptive. In the current study, we developed an automatic segmentation model using a deep learning system, U-Net, and subtraction of the retinal vessel area between early-phase and late-phase FA images for the detection of the retinal vascular leakage area in ultrawide field (UWF) FA images in three patients with Behçet’s Disease and three patients with idiopathic uveitis with retinal vasculitis. This study demonstrated that the automated model for segmentation of the retinal vascular leakage area through the UWF FA images reached 0.434 (precision), 0.529 (recall), and 0.467 (Dice coefficient) without using UWF FA images for training. There was a significant positive correlation between the automated segmented area (pixels) of retinal vascular leakage and the FA vascular leakage score. The mean pixels of automatic segmented vascular leakage in UWF FA images with treatment was significantly reduced compared with before treatment. The automated segmentation of retinal vascular leakage in UWF FA images may be useful for objective and quantitative assessment of disease activity in posterior segment uveitis. Further studies at a larger scale are warranted to improve the performance of this automatic segmentation model to detect retinal vascular leakage.
Surface Subsidence Monitoring in Kunming City with Time-Series InSAR and GNSS
Authors: Zhu Shasha , Zuo Xiaoqing , Shi Ke , Li Yongfa , Guo Shipeng , Li Chen
Published: December 2022

Kunming city is located in the middle of Yunnan Province. Due to large-scale groundwater exploitation and urban development in recent years, this area has been affected by surface subsidence. In this paper, Interferometric Synthetic Aperture Radar (InSAR) and Global Navigation Satellite System (GNSS) data are used to monitor the surface subsidence in Kunming city area for better analysis and understanding. The study used data of Sentinel-1A from 2018 to 2020 with atmospheric correction based on GACOS to calculate the average annual subsidence rate in Kunming city area, and the results show that the maximum subsidence rate is 48 mm/year. The subsidence obtained by InSAR is compared with the vertical deformation information obtained by eight GNSS stations in continuous operation in the study area. The subsidence rate trend show by the two methods is consistent, which further verifies the validity of InSAR data to reflect the local deformation. Experimental results shown that the eastern and northeastern Dianchi lake areas were affected by underground resources mining, and the induced surface subsidence characteristics were obvious, with the surface subsidence rate reachde 48 mm/year and 37 mm/year respectively. The Kunyang Phosphate Mine also had different degrees of mining subsidence disaster, with the maximum subsidence rate reached 36 mm/year. The subsidence rate of InSAR and GNSS has the same trend on the whole. However, GNSS sites are generally located in stable areas, the settlement amount obtained in the same time period is somewhat different from that of InSAR.
Tracking the Biogenic Component of Lower-Carbon Intensive, Co-Processed Fuels—An Overview of Existing Approaches
Authors: O’Connell Adrian , Su Jianping , Ringsred Anna , Prussi Matteo , Saddler Jack , Scarlat Nicolae
Published: December 2022

Several methods are currently used to track the bio-component of co-processed fuels including energy/mass balance, yield methods and radiocarbon techniques. The methods used to track or estimate the bio-component of fuels produced when bio and fossil feedstocks are processed together (co-processed) in oil refineries were analysed in detail, together with their advantages and disadvantages. Some methods, such as radiocarbon methods that allow the direct measurement of the bio-content in a fuel, have been criticised due to low accuracy at low blends. However, these reservations have tended to misinterpret the options available for carbon dating and to discount recent improvements in these tests. As much higher co-pressing mixtures are anticipated if published national decarbonisation targets are to be achieved, any challenges at very low co-processing ratios affecting the accuracy of the radiocarbon methods should not be an issue. Energy/mass balance and yield methods might be supplemented with carbon-tracking to determine the real the biogenic content.