bluuebunny/crossref_metadata_2025 huggingface dataset:
Diagnosis of Breast Hyperplasia and Evaluation of RuXian-I Based on Metabolomics Deep Belief Networks
Authors: Jiang Mingyang , Liang Yanchun , Pei Zhili , Wang Xiye , Zhou Fengfeng , Wei Chengxi , Feng Xiaoyue
Published: May 2019

Breast cancer is estimated to be the leading cancer type among new cases in American women. Core biopsy data have shown a close association between breast hyperplasia and breast cancer. The early diagnosis and treatment of breast hyperplasia are extremely important to prevent breast cancer. The Mongolian medicine RuXian-I is a traditional drug that has achieved a high level of efficacy and a low incidence of side effects in its clinical use. However, for detecting the efficacy of RuXian-I, a rapid and accurate evaluation method based on metabolomic data is still lacking. Therefore, we proposed a framework, named the metabolomics deep belief network (MDBN), to analyze breast hyperplasia metabolomic data. We obtained 168 samples of metabolomic data from an animal model experiment of RuXian-I, which were averaged from control groups, treatment groups, and model groups. In the process of training, unlabelled data were used to pretrain the Deep Belief Networks models, and then labelled data were used to complete fine-tuning based on a limited-memory Broyden Fletcher Goldfarb Shanno (L-BFGS) algorithm. To prevent overfitting, a dropout method was added to the pretraining and fine-tuning procedures. The experimental results showed that the proposed model is superior to other classical classification methods that are based on positive and negative spectra data. Further, the proposed model can be used as an extension of the classification method for metabolomic data. For the high accuracy of classification of the three groups, the model indicates obvious differences and boundaries between the three groups. It can be inferred that the animal model of RuXian-I is well established, which can lay a foundation for subsequent related experiments. This also shows that metabolomic data can be used as a means to verify the effectiveness of RuXian-I in the treatment of breast hyperplasia.
Involvement of G-Protein-Coupled Receptor 40 in the Inhibitory Effects of Docosahexaenoic Acid on SREBP1-Mediated Lipogenic Enzyme Expression in Primary Hepatocytes
Authors: On Seungtae , Kim Hyun Young , Kim Hyo Seon , Park Jeongwoo , Kang Keon Wook
Published: May 2019

Nonalcoholic fatty liver disease is a frequent liver malady, which can progress to cirrhosis, the end-stage liver disease if proper treatment is not applied. Omega-3 fatty acids, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid, have been clinically proven to lower serum triglyceride levels. Various physiological activities of omega-3 fatty acids are due to their agonistic actions on G-protein-coupled receptor 40 (GPR40) and GPR120. Lipid droplets (LD) accumulation in hepatocytes confirmed that DHA treatment reduced the number of larger ( >10 μm2) LDs, as well as the total area of LDs. Moreover, DHA lowered protein and mRNA expression levels of lipogenic enzymes such as fatty acid synthase (FAS), acetyl-CoA carboxylase and stearoyl-CoA desaturase-1 (SCD-1) in primary hepatocytes incubated with liver X receptor (LXR) agonist T0901317 or high glucose and insulin. DHA also decreased protein expression of nuclear and precursor sterol response-element binding protein (SREBP)-1, a key lipogenesis transcription factor. We further found that exposure of murine primary hepatocytes to DHA for 12 h increased GPR40 and GPR120 mRNA levels. Specific agonists (Compound A for GPR120 and AMG-1638 for GPR40), hepatocytes from GPR120 knock-out mice and GPR40 selective antagonist (GW1100) were used to assess whether DHA’s antilipogenic effects are mediated through GPR120 or GPR40. Compound A did not decrease SREBP-1 and FAS protein expression in hepatocytes exposed to T0901317 or high glucose with insulin. Moreover, DHA downregulated lipogenesis enzyme expression in GPR120-null hepatocytes. In contrast, AMG-1638 lowered SREBP-1 and SCD-1 protein levels. Additionally, GW1100, a GPR40 antagonist, reversed the antilipogenic effects of DHA. Collectively, our data demonstrate that DHA downregulates the expression SREBP-1-mediated lipogenic enzymes via GPR40 in primary hepatocytes.
Stemarane Diterpenes and Diterpenoids
Authors: Leonelli Francesca , Valletta Alessio , Migneco Luisa Maria , Marini Bettolo Rinaldo
Published: May 2019

In this article the scientific activity carried out on stemarane diterpenes and diterpenoids, isolated over the world from various natural sources, was reviewed. The structure elucidation of stemarane diterpenes and diterpenoids was reported, in addition to their biogenesis and biosynthesis. Stemarane diterpenes and diterpenoids biotransformations and biological activity was also taken into account. Finally the work leading to the synthesis and enantiosynthesis of stemarane diterpenes and diterpenoids was described.
Recent Progress on Catalysts for the Positive Electrode of Aprotic Lithium-Oxygen Batteries †
Authors: Cai Yichao , Hou Yunpeng , Lu Yong , Chen Jun
Published: May 2019

Rechargeable aprotic lithium-oxygen (Li-O2) batteries have attracted significant interest in recent years owing to their ultrahigh theoretical capacity, low cost, and environmental friendliness. However, the further development of Li-O2 batteries is hindered by some ineluctable issues, such as severe parasitic reactions, low energy efficiency, poor rate capability, short cycling life and potential safety hazards, which mainly stem from the high charging overpotential in the positive electrode side. Thus, it is of great significance to develop high-performance catalysts for the positive electrode in order to address these issues and to boost the commercialization of Li-O2 batteries. In this review, three main categories of catalyst for the positive electrode of Li-O2 batteries, including carbon materials, noble metals and their oxides, and transition metals and their oxides, are systematically summarized and discussed. We not only focus on the electrochemical performance of batteries, but also pay more attention to understanding the catalytic mechanism of these catalysts for the positive electrode. In closing, opportunities for the design of better catalysts for the positive electrode of high-performance Li-O2 batteries are discussed.
Landscape—A Review with a European Perspective
Authors: Talento Katia , Amado Miguel , Kullberg Josè Carlos
Published: May 2019

This article aims to act as a general literature review regarding the landscape, analyzing it through a synthesis of the main concepts and processes that have generated, and subsequently developed, the word “Landscape”. It is a versatile theme, because it has always been studied by various disciplines, through different theories, which sometimes even conflict with each other. Through the present text, we understand the importance and the unique value of the landscape, a value that has nowadays been transfigured by the strong industrialization and strong brand of man in the territory. Thus, the first part of the research is, to some extent, a reflection on current issues that are related to the landscape. It is also a tool for integration, including in the definition of “Landscape”, even those heavily humanized, exploited, degraded, abandoned, and residual; the so-called “Drosscape”, “Friche”, and “Terrain Vague”. The solution is not to negatively interpret these types of scenarios, but rather to enhance them as they are, filled with potential and creativity. This concept is achieved by means of an operation of recycling or reuse of waste, which is capable of germinating new life cycles within the “dead nature” of our increasingly cemented territories.
Human Osteoblast Cell Behaviour on Titanium Discs Treated with Argon Plasma
Authors: González-Blanco Carolina , Rizo-Gorrita María , Luna-Oliva Irene , Serrera-Figallo María-Ángeles , Torres-Lagares Daniel , Gutiérrez-Pérez José-Luis
Published: May 2019

(1) Background. Titanium is characterized by its biocompatibility and resistance to stress and fatigue. Treatment with argon plasma may favour growth of human osteoblasts with respect to cell adhesion and proliferation. The aim of this study was to analyse the behaviour of human osteoblasts (MG-63) on Grade IV and V titanium possessing a sand-blasted, acid-etched (SLA) surface. SLA is a widely used surface treatment to create micro- and macroretentions to enhance osteoconductive properties on the surface. (2) Methods. One group of each grade of titanium was decontaminated with argon plasma and compared. On each disc, 20 × 104 cells were cultivated for morphological analysis, study of cell viability (regarding a negative control [100% viability]) and mitochondrial energy balance. (3) Results. At 24 h titanium treated with SLA showed a higher percentage of cell viability (47.3 ± 8.1%) compared to titanium IV treated with argon plasma, which presented a percentage of 79.1 ± 1.1%. Grade V titanium treated with argon plasma presented a higher viability percentage 91.3 ± 3.0% whereas nontreated Grade V titanium presented 53.3 ± 4.0%. Cells cultivated on the surfaces with an argon-plasma treatment were enlarged in comparison to non-treated discs. The cells with smaller circularity with a greater spread and spindle shape were the ones cultivated on the Grade V titanium surface. Cells seeded on treated titanium IV and titanium V, treated or not, showed higher mitochondrial activity over nontreated titanium IV. (4) Conclusions. Cells cultivated on those Grade V titanium discs that were decontaminated with argon plasma presented higher levels of cell adhesion and proliferation, lower mitochondrial damage and a higher mean cell area compared to those not decontaminated with argon plasma.
Epidemiological Study of Lung Cancer Incidence in Lebanon
Authors: Salhab Hamza A. , Fares Mohamad Y. , Khachfe Hussein H. , Khachfe Hassan M.
Published: May 2019

Background and Objectives: Lung cancer (LC) is the most common cancer in the world. Developing countries in the Middle East and North Africa (MENA) region, including Lebanon, have witnessed a great increase in the incidence rates of this disease. The aim of our study is to investigate the incidence rates of lung cancer in Lebanon from 2005 to 2015 and to compare these rates to other countries from the MENA region and other regions of the world. Material and Methods: Lung cancer data for the years 2005–2015 were collected from the National Cancer Registry of Lebanon and stratified by gender and age group. Age-specific and age-standardized incidence rates were calculated and analyzed using joinpoint regression. Age-standardized incidence rates to the world population (ASR(w)) for other countries were obtained from two online databases. Results: Lung cancer ranked as the second most common cancer in Lebanon and accounted for 9.2% of all newly diagnosed cancers. Lung cancer ASR(w) showed a significantly increasing trend over the period studied for males and females. Lung cancer ASR(w) among males in Lebanon came second after Malta when compared to other MENA countries, but it was among the lowest when compared to non-MENA countries. For females, Lebanon ranked first when compared to other MENA countries but was among the lowest when compared to countries in other regions of the world. The lung cancer incidence rate increased with age in both sexes and 89.2% of patients were 50 years of age or older. Conclusion: Lebanon has the highest incidence of LC in females and the second highest for males in the MENA region. The lung cancer incidence rate is on the rise and older age groups are much more burdened by this disease than the young ones. Several risk factors, particularly smoking, play a role in increased LC incidence among the Lebanese population.
What Makes Bariatric Operations Difficult–Results of a National Survey
Authors: Major Piotr , Stefura Tomasz , Walędziak Maciej , Janik Michał , Pędziwiatr Michał , Wysocki Michał , Rubinkiewicz Mateusz , Witowski Jan , Szeliga Jacek , Budzyński Andrzej
Published: May 2019

Background and objective: The most commonly performed bariatric procedures include laparoscopic sleeve gastrectomy (LSG), laparoscopic Roux-en-Y gastric bypass (LRYGB), and one anastomosis gastric bypass-mini gastric bypass (OAGB-MGB). A study comparing the degree of difficulty among those procedures could serve as a guide for decision making in bariatric surgery and further improve training programs for general surgery trainees. The aim of this study was to compare the subjective level of technical difficulty of LSG, LRYGB, and OAGB–MGB as perceived by surgeons and surgical residents. Materials and Methods: An anonymous internet-based survey was designed to evaluate the subjective opinions of surgeons and surgical residents in training in Poland. It covered baseline characteristics of the participants, difficulty of LSG, OAGB-MGB, LRYGB and particular stages of each operation assessed on a 1–5 scale. Results: Overall, 70 surgeons and residents participated in our survey. The mean difficulty degree of LSG was 2.34 ± 0.89. The reinforcing staple line with sutures was considered most difficult stage of this operation (3.17 ± 1.19). The LRYGB operation had an average difficulty level of 3.87 ± 1.04. Creation of the gastrojejunostomy was considered the most difficult stage of LRYGB with a mean difficulty level (3.68 ± 1.16). Responders to our survey assessed the mean degree of difficulty of OAGB-MGB as 2.34 ± 0.97. According to participating surgeons, creating the gastrojejunostomy is the most difficult phase of this operation (3.68 ± 1.16). Conclusion: The LSG is perceived by surgeons as a relatively easy operation. The LRYGB was considered to be the most technically challenging procedure in our survey. Operative stages, which require intra-abdominal suturing with laparoscopic instruments, seem to be the most difficult phases of each operation.
Associations of Cognitive Function with BMI, Body Fat Mass and Visceral Fat in Young Adulthood
Authors: Huang Tao , Chen Zuosong , Shen Liqun , Fan Xiang , Wang Kun
Published: May 2019

Background and objectives: Existing studies concerning the associations of cognitive function with adiposity in young adults are sparse. The purpose of the study was to examine the associations of adiposity with cognitive control in young adults. Materials and Methods: Participants were 213 young adults (98 women and 115 men). Cognitive control was measured using a modified task-switching paradigm. Anthropometrics were measured by standardized procedures. Body fat mass and visceral fat area were measured by bioelectrical impedance analysis. Results: The results showed that increased body mass index (BMI, p = 0.02), body fat percentage (p = 0.02), and visceral fat area (p = 0.01) were significantly correlated with larger global switch costs of accuracy in women. In men, high levels of body fat percentage (p = 0.01) and visceral fat area (p = 0.03) were significantly correlated with larger local switch costs of reaction time. Conclusions: The results indicated that elevated adiposity was associated with worse performance on measures of cognitive control in young adults.
A Comprehensive Study on Fitness Approximation Techniques in Shape Optimization of Aerofoil Forging Preform Tools
Authors: Shao Yong , Yan Lin , Guo Pingyi , Yang Hongyu , Shi Fengjian , Feng Di
Published: May 2019

In the process of complex engineering designs or optimizations, a large number of physical experiments or numerical simulations are required to evaluate certain performance qualities before a satisfactory result can be obtained. In both cases, constructing an approximate model is often necessary to provide a reliable response as an alternative to experiments or simulations. In this paper, three types of approximation models were developed and applied in a shape design of an aerofoil forging preform tool. Their modeling techniques are presented in detail. An optimal Latin hypercube technique was employed for the design of the experiment and sampling with the expected coverage of parameter space. Finite element (FE) simulations of multistep forging processes were implemented to acquire the objective function values for evaluating the forging performance. By a parametric study, the effects of design variables on objective responses and correlations were investigated for a clear insight into their functional nature. Comprehensive analyses and comparisons between different approximate models have been carried out. Finally, an optimization design of a preform tool was successfully achieved based on a particle swarm (PSO) algorithm combined with the proposed approximate model.
Functionally Graded SS 316L to Ni-Based Structures Produced by 3D Plasma Metal Deposition
Authors: Rodriguez Johnnatan , Hoefer Kevin , Haelsig Andre , Mayr Peter
Published: May 2019

In this investigation, the fabrication of functionally graded structures of SS316L to Ni-based alloys were studied, using the novel technique 3D plasma metal deposition. Two Ni-based alloys were used, a heat resistance alloy Ni80-20 and the solid-solution strengthened Ni625. Different configurations were analyzed, for the Ni80-20 a hard transition and a smooth transition with a region of 50% SS316L/50% Ni80-20. Regarding the structures with Ni625, a smooth transition configuration and variations in the heat input were applied. The effect of the process parameters on the geometry of the structures and the microstructures was studied. Microstructure examinations were carried out using optical and scanning electron microscopy. In addition, microhardness analysis were made on the interfaces. In general, the smooth transition of both systems showed a gradual change in the properties. The microstructural results for the SS316L (both systems) showed an austenite matrix with δ-phase. For the mixed zone and the Ni80-20 an austenite (γ) matrix with some M7C3 precipitates and laves phase were recognized. The as-built Ni625 microstructure was composed of an austenite (γ) matrix with secondary phases laves and δ-Ni3Nb, and precipitates M7C3. The mixed zone exhibited the same phases but with changes in the morphology.
Numerical Simulation of Material Flow and Analysis of Welding Characteristics in Friction Stir Welding Process
Authors: Luo Haitao , Wu Tingke , Wang Peng , Zhao Fengqun , Wang Haonan , Li Yuxin
Published: May 2019

Friction stir welding (FSW) material flow has an important influence on weld formation. The finite element model of the FSW process was established. The axial force and the spindle torque of the welding process were collected through experiments. The feasibility of the finite element model was verified by a data comparison. The temperature field of the welding process was analyzed hierarchically. It was found that the temperature on the advancing side is about 20 °C higher than that on the retreating side near the welding seam, but that the temperature difference between the two sides of the middle and lower layers was decreased. The particle tracking technique was used to study the material flow law in different areas of the weld seam. The results showed that part of the material inside the tool pin was squeezed to the bottom of the workpiece. The material on the upper surface tends to move downward under the influence of the shoulder extrusion, while the material on the lower part moves spirally upward under the influence of the tool pin. The material flow amount of the advancing side is higher than that of the retreating side. The law of material flow reveals the possible causes of the welding defects. It was found that the abnormal flow of materials at a low rotation speed and high welding speed is prone to holes and crack defects. The forming reasons and material flow differences in different regions are studied through the microstructure of the joint cross section. The feasibility of a finite element modeling and simulation analysis is further verified.
A Novel Seedless TSV Process Based on Room Temperature Curing Silver Nanowires ECAs for MEMS Packaging
Authors: Meng Min , Cheng Lijuan , Yang Kai , Sun Mingyan , Luo Yi
Published: May 2019

The through-silicon-vias (TSVs) process is a vital technology in microelectromechanical systems (MEMS) packaging. The current via filling technique based on copper electroplating has many shortcomings, such as involving multi-step processes, requiring sophisticated equipment, low through-put and probably damaging the MEMS devices susceptible to mechanical polishing. Herein, a room temperature treatable, high-efficient and low-cost seedless TSV process was developed with a one-step filling process by using novel electrically conductive adhesives (ECAs) filled with silver nanowires. The as-prepared ECAs could be fully cured at room temperature and exhibited excellent conductivity due to combining the benefits of both polymethyl methacrylate (PMMA) and silver nanowires. Complete filling of TSVs with the as-prepared 30 wt% silver nanowires ECAs was realized, and the resistivity of a fully filled TSV was as low as 10−3 Ω·cm. Furthermore, the application of such novel TSV filling process could also be extended to a wide range of different substrates, showing great potential in MEMS packaging, flexible microsystems and many other applications.
Transcriptional Expression of the ompA, cpaf, tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle
Authors: Korhonen Suvi , Hokynar Kati , Mannonen Laura , Paavonen Jorma , Hiltunen-Back Eija , Puolakkainen Mirja
Published: May 2019

The transcriptional gene expression patterns of Chlamydia trachomatis have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number C. trachomatis clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the ompA, cpaf, tarp, and tox genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of ompA and the peak of the tox expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of cpaf was high in the reference strains compared with the clinical isolates at the mid-phase (6–24 hours post infection) of the developmental cycle. All of the strains had a rather similar tarp expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number C. trachomatis clinical isolates instead of reference strains in the studies might better reflect the situation in human infection.
Electrochemical Reduction of CO2 to Formate on Easily Prepared Carbon-Supported Bi Nanoparticles
Authors: Ávila-Bolívar Beatriz , García-Cruz Leticia , Montiel Vicente , Solla-Gullón José
Published: May 2019

Herein, the electrochemical reduction of CO2 to formate on carbon-supported bismuth nanoparticles is reported. Carbon-supported Bi nanoparticles (about 10 nm in size) were synthesized using a simple, fast and scalable approach performed under room conditions. The so-prepared Bi electrocatalyst was characterized by different physicochemical techniques, including transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction and subsequently air-brushed on a carbon paper to prepare electrodes. These electrodes were characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy and also by cyclic voltammetry. Finally, CO2 electroreduction electrolyses were performed at different electrode potentials for 3 h. At the optimal electrode potential (−1.6 V vs AgCl/Ag), the concentration of formate was about 77 mM with a faradaic efficiency of 93 ± 2.5%. A 100% faradaic efficiency was found at a lower potential (−1.5 V vs AgCl/Ag) with a formate concentration of about 55 mM. In terms of stability, we observed that after about 70 h (in 3 h electrolysis experiments at different potentials), the electrode deactivates due to the gradual loss of metal as shown by SEM/EDX analyses of the deactivated electrodes.
Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model
Authors: Douglas A. Araújo Hallysson , G. Silva Júnior José , R. Saturnino Oliveira João , Helena M. L. Ribeiro Maria , C. Barroso Martins Mônica , A. Cavalcanti Bezerra Marcos , Lima Aires André , C. P. Azevedo Albuquerque Mônica , R. Melo-Júnior Mário , T. Pontes Filho Nicodemos , C. Pereira Eugênia , J. Raposo Silva Diego , V. dos Anjos Janaína , Peter S. Falcão Emerson , H. Silva Nicácio , L. Menezes Lima Vera
Published: May 2019

To obtain usnic acid potassium salt (PS-UA), the usnic acid (UA) was extracted and purified from the lichen Cladonia substellata, and modified to produce PS-UA. The structure was determined by 1H-NMR, IR and elemental analysis, ratified through computational models, as well as identification the site of K+ insertion in the molecule. Antinociceptive activity was detected through contortions in mice induced by acetic acid and formalin (phases I and II) after treatments with 10 and 20 mg/kg of PS-UA, indicating interference in both non-inflammatory and inflammatory pain. After oral administration at doses of 500, 1000 and 2000 mg/kg, no deaths of mice with treatments below 2000 mg/kg were observed. Except for body weight gain, food and water consumption decreased with treatments of 1000 and 2000 mg/kg, and the number of segmented leukocytes was higher for both treatments. Regarding serum levels, cholesterol and triglycerides decreased, however, there was an increase in hepatic transaminases with both treatments. Liver and kidney histological changes were detected in treatments of 2000 mg/kg, while the spleen was preserved. The PS-UA demonstrated antinociceptive activity while the acute toxicity at the concentration of 2000 mg/kg was the only dose that presented morphological changes in the liver and kidney.
Hydration, Arginine Vasopressin, and Glucoregulatory Health in Humans: A Critical Perspective
Authors: Carroll Harriet A. , James Lewis J.
Published: May 2019

Glucoregulatory diseases, such as type 2 diabetes are currently a key public health priority. Public health messages have started to include the addition of water in their dietary guidelines. Such guidelines however are not based on causal evidence pertaining to the health effects of increased water intake, but rather more heavily based upon non-causal or mechanistic data. One line of thinking linking fluid intake and health is that hypohydration induces elevated blood concentrations of arginine vasopressin (AVP). Research in the 1970s and 1980s implicated AVP in glucoregulation, supported by observational evidence. This important area of research subsequently appeared to stop until the 21st century during which interest in hypertonic saline infusion studies, animal AVP receptor knockout models, dietary and genetic associations, and human interventions manipulating hydration status have resurged. This narrative review briefly describes and critically evaluates the usefulness of the current AVP-glucoregulatory research. We offer suggestions on how to test the independent glucoregulatory effects of body water changes compared to elevated circulating AVP concentrations, such as investigating hydration manipulations using 3,4-Methylenedioxymethamphetamine. Whilst much research is still needed before making firm conclusions, the current evidence suggests that although AVP may be partially implicated in glucoregulation, more ecologically valid models using human participants suggests this effect might be independent of the hydration status. The key implication of this hypothesis if confirmed in future research is that manipulating the hydration status to reduce circulating AVP concentrations may not be an effective method to improve glucoregulatory health.
Consumption of Yogurt in Canada and Its Contribution to Nutrient Intake and Diet Quality Among Canadians
Authors: Vatanparast Hassan , Islam Naorin , Patil Rashmi Prakash , Shamloo Arash , Keshavarz Pardis , Smith Jessica , Whiting Susan
Published: May 2019

The current study utilizes a nationally representative nutrition survey data (Canadian Community Health Survey 2015, nutrition component, n = 20,487) in order to evaluate patterns of yogurt consumption among Canadians. Overall, 20% of Canadians have reportedly consumed yogurt on a given day in 2015. Higher prevalence of yogurt consumption was noted among children aged 2–5 years old (47%) when compared to adults aged 19–54 years (18%). When the prevalence of yogurt consumption at the regional level in Canada was assessed, Quebec had the most consumers of yogurt (25%) compared to other regions, namely the Atlantic (19%), Ontario (18%), Prairies (19%) and British Columbia (20%). Yogurt consumers reported consuming higher daily intakes of several key nutrients including carbohydrates, fibre, riboflavin, vitamin C, folate, vitamin D, potassium, iron, magnesium, and calcium when compared to yogurt non-consumers. Additionally, the diet quality, measured using NRF 9.3 scoring method, was higher among yogurt consumers compared to non-consumers. Nearly 36% of Canadians who meet the dietary guidelines for milk and alternative servings from the Food Guide Canada (2007) reported consuming yogurt. Lastly, no significant difference in BMI was noted among yogurt consumers and non-consumers. Overall, yogurt consumers had a higher intake of key nutrients and had a better diet quality.
Reformulation and Priorities for Reducing Energy Density; Results from a Cross-Sectional Survey on Fat Content in Pre-Packed Cakes and Biscuits Sold in British Supermarkets
Authors: Alessandrini Roberta , He Feng J. , Hashem Kawther M. , Tan Monique , MacGregor Graham A.
Published: May 2019

Cakes and biscuits contribute to energy, total and saturated fat and sugar in British diets. So far, the UK government has prompted manufacturers to reduce energy density in these products through a reduction of their sugar content. We conducted a cross-sectional survey of the fat content of cakes and biscuits available in nine UK supermarket chains. In cakes (n = 381), the mean total fat content was 17.9 ± 5.2 g/100 g (39% of the overall energy); range (1.4–35.6 g/100 g) and the average saturated fat content in cakes was 5.9 ± 3.4 g/100 g (13% of the overall energy); range (0.3–20 g/100 g). In biscuits (n = 481), the mean total fat content was 21.8 g ± 6.3 g/100 g (40% of the overall energy); range (0.7–38.9 g/100 g) and the average saturated fat content was 11.4 ± 4.9 g/100 g (23% of the overall energy); range (0.3–22.3 g/100 g). In both cakes and biscuits, total and saturated fat content was positively correlated with energy density. Our results show that cakes and biscuits sold in UK supermarkets are high in total and saturated fat, and that fat content contributes substantially to product energy density. Fat reformulation in these products would effectively reduce energy density, calorie intake and help prevent obesity. Fat reformulation should be implemented simultaneously with sugar reformulation and be focused on saturated fat, as this will have the additional effect of lowering LDL cholesterol.
Quantum Vacuum: The Structure of Empty Space–Time and Quintessence with Gauge Symmetry Group SU(2) ⊗ U(1)
Authors: Gevorkyan Ashot
Published: May 2019

We consider the formation of structured and massless particles with spin 1, by using the Yang–Mills-like stochastic equations system for the group symmetry S U ( 2 ) ⊗ U ( 1 ) without taking into account the nonlinear term characterizing self-action. We prove that, in the first phase of relaxation, as a result of multi-scale random fluctuations of quantum fields, massless particles with spin 1, further referred as hions, are generated in the form of statistically stable quantized structures, which are localized on 2D topological manifolds. We also study the wave state and the geometrical structure of the hion when as a free particle and, accordingly, while it interacts with a random environment becoming a quasi-particle with a finite lifetime. In the second phase of relaxation, the vector boson makes spontaneous transitions to other massless and mass states. The problem of entanglement of two hions with opposite projections of the spins + 1 and − 1 and the formation of a scalar zero-spin boson are also thoroughly studied. We analyze the properties of the scalar field and show that it corresponds to the Bose–Einstein (BE) condensate. The scalar boson decay problems, as well as a number of features characterizing the stability of BE condensate, are also discussed. Then, we report on the structure of empty space–time in the context of new properties of the quantum vacuum, implying on the existence of a natural quantum computer with complicated logic, which manifests in the form of dark energy. The possibilities of space–time engineering are also discussed.
Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening
Authors: Jara Karla , Castro Ricardo I. , Ramos Patricio , Parra-Palma Carolina , Valenzuela-Riffo Felipe , Morales-Quintana Luis
Published: May 2019

The endo-β-1,4-glucanases (EGs) that belong to the glycosyl hydrolase family 9 (GH9) have roles in cell wall synthesis, remodeling and degradation. Previous studies have suggested that EGs may play a key role in the ripening of different fruits including strawberries. In this study, we used reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assays to determine the transcript accumulation of an endo-β-1,4-glucanase (FaEG1) during fruit development in two different strawberry ‘Camarosa’ and ‘Monterey’ with contrasting softening ratios. Phylogenetic analyses suggest that FaEG1 belongs to the α group of the GH9 family with other proteins previously described with roles in elongation, abscission and ripening. Comparative modeling was used to obtain the FaEG1 structure. The model displays a α-barrel–type structure that is typical of the GH9 enzyme family, and comprises 12 α-helices, 2 310 helices and 6 β-sheets. The catalytic residues were oriented to the solvent in the middle of an open groove. Protein–ligand interactions were explored with cellulose and two xyloglucans as ligands; the results suggest that the FaEG1-cellulose and FaEG1-XXXGXXXG (the most abundant xyloglucan in strawberries) complexes were more stable complexes than XXFGXXFG. The cell wall degradation was observed by scanning electron microscopy (SEM). The data are congruent with the probable role of the FaEG1 protein in the dissembly of the cellulose-hemicellulose fraction during the ripening of strawberry fruit.
Removal of Linear and Branched Alkylphenols with the Combined Use of Polyphenol Oxidase and Chitosan
Authors: Tsushima Mitsuki , Kimura Yuji , Kashiwada Ayumi , Yamada Kazunori
Published: May 2019

Removal of linear and branched alkylphenols with different alkyl chain lengths or different branchings (normal, secondary, and tertiary), some of which are suspected as endocrine disrupting chemicals, from an aqueous medium were investigated through quinone oxidation by polyphenol oxidase (PPO) and subsequent quinone adsorption on chitosan beads or powders at pH 7.0 and 40 °C. PPO-catalyzed quinone oxidation increased with an increase in alkyl chain length of the alkylphenols used. Although a higher PPO dose was required for quinone oxidation of branched alkylphenols, they were completely or mostly removed by quinone adsorption on chitosan beads or powders. The apparent activity of PPO increased by a decrease in quinone concentration. On the other hand, in the homogeneous systems with solutions of chitosan and PPO at pH 6.0, longer reaction times were required to generate insoluble aggregates, and a small amount of quinone derivatives were left in the solution even under optimum conditions. These results support that the two-step reaction, that is, PPO-catalyzed quinone oxidation and subsequent quinone adsorption on chitosan beads or powders, in the heterogeneous system is a good procedure for removing linear and branched alkylphenols from aqueous medium.
Dynamic Modelling and Optimisation of the Batch Enzymatic Synthesis of Amoxicillin
Authors: Cuthbertson Andrew B. , Rodman Alistair D. , Diab Samir , Gerogiorgis Dimitrios I.
Published: May 2019

Amoxicillin belongs to the β-lactam family of antibiotics, a class of highly consumed pharmaceutical products used for the treatment of respiratory and urinary tract infections, and is listed as a World Health Organisation (WHO) “Essential Medicine”. The demonstrated batch enzymatic synthesis of amoxicillin is composed of a desired synthesis and two undesired hydrolysis reactions of the main substrate (6-aminopenicillanic acid (6-APA)) and amoxicillin. Dynamic simulation and optimisation can be used to establish optimal control policies to attain target product specification objectives for bioprocesses. This work performed dynamic modelling, simulation and optimisation of the batch enzymatic synthesis of amoxicillin. First, kinetic parameter regression at different operating temperatures was performed, followed by Arrhenius parameter estimation to allow for non-isothermal modelling of the reaction network. Dynamic simulations were implemented to understand the behaviour of the design space, followed by the formulation and solution of a dynamic non-isothermal optimisation problem subject to various product specification constraints. Optimal reactor temperature (control) and species concentration (state) trajectories are presented for batch enzymatic amoxicillin synthesis.
Entanglement Dynamics of Three and Four Level Atomic System under Stark Effect and Kerr-Like Medium
Authors: Anwar S. Jamal , Ramzan M. , Usman M. , Khan M. Khalid
Published: May 2019

We investigated numerically the dynamics of quantum Fisher information (QFI) and entanglement for three- and four-level atomic systems interacting with a coherent field under the effect of Stark shift and Kerr medium. It was observed that the Stark shift and Kerr-like medium play a prominent role during the time evolution of the quantum systems. The non-linear Kerr medium has a stronger effect on the dynamics of QFI as compared to the quantum entanglement (QE). QFI is heavily suppressed by increasing the value of Kerr parameter. This behavior was found comparable in the cases of three- and four-level atomic systems coupled with a non-linear Kerr medium. However, QFI and quantum entanglement (QE) maintain their periodic nature under atomic motion. On the other hand, the local maximum value of QFI and von Neumann entropy (VNE) decrease gradually under the Stark effect. Moreover, no prominent difference in the behavior of QFI and QE was observed for three- and four-level atoms while increasing the value of Stark parameter. However, three- and four-level atomic systems were found equally prone to the non-linear Kerr medium and Stark effect. Furthermore, three- and four-level atomic systems were found fully prone to the Kerr-like medium and Stark effect.
Why Teach about Religions? Perspectives from Finnish Professionals
Authors: Kuusisto Arniika , Gearon Liam
Published: May 2019

Acknowledging recent research literature on professionalism and religious education across Europe, the article examines the scholars’ and senior professionals’ views on the curricula aims and objectives in religious education in Finland. Through asking the professionals’ views on the aims of RE in relation to supporting of child’s growth and development on one hand and the societal aims of RE on the other, the findings were thematically classified into the following categories. Firstly, the aims regarding the supporting of child’s growth and development were focused on literacy on religions and worldviews, increasing the understanding on oneself and others, personal growth, and the skills for global citizenship. From the societal perspective, RE was seen important for supporting the understanding as literacy, understanding as empathy, and competences for global citizenship. Finally, as regards the educational model of teaching about religions, these professionals held somewhat varied views. Some favoured an RE model based on teaching groups reflecting children’s own worldview affiliations, others supported whole-class instruction, and still others a hybrid model combining elements of both. However, the way in which the instruction is implemented and the position from which religions are examined in education were perceived to be in a key role in this, whatever the formal structures for instruction.
Biomass Sources and Energy Potential for Energy Sector in Myanmar: An Outlook
Authors: Tun Maw Maw , Juchelková Dagmar
Published: May 2019

Nowadays, renewable energy utilization plays a key role in developing countries to fulfill the additional energy requirements of a country and reduce dependency on fossil fuels and traditional biomass consumption. As Myanmar has an agriculture-based economy and 48% of forest-cover (32.2 million hectares); biomass is one of the major renewable energy sources, contributing around 50% of total energy consumption. Therefore, the study aimed to highlight the available biomass sources and energy potential for the energy sector in Myanmar. In order to achieve the aim, the study collated the types, quantity and qualities of biomass resources, and energy utilization around Myanmar. Besides, the study synthesized and evaluated the energy potential of the major biomass resources coming from the agriculture sector, forest sector, livestock and poultry sector, and municipal sector. It was estimated that the total energy potential of the major biomass sources amounted to approximately 15.19 million tons of oil equivalent (Mtoe) in 2005 and 17.29 Mtoe in 2017, respectively. The unexploited biomass energy potential around the country was estimated to be nearly 50% higher than that of the projected biomass energy utilization during 2015–2019. Finally, the study concluded with recommendations to provide the future sustainable development of biomass energy in Myanmar.
Long-Term Deflection Monitoring for Bridges Using X and C-Band Time-Series SAR Interferometry
Authors: Jung Jungkyo , Kim Duk-jin , Palanisamy Vadivel Suresh Krishnan , Yun Sang-Ho
Published: May 2019

This study aims to monitor the deformation of bridges, namely in the form of long-term deflection and thermal dilation, using multi-temporal interferometric synthetic aperture radar (InSAR) observations. To precisely estimate the vertical and longitudinal displacements, we used the InSAR time-series technique with multi-track stacks of Sentinel-1 SAR dataset and a single-track stack of COSMO-SkyMed SAR data over two extradosed bridge cases; Kimdaejung and Muyoung bridges between 2013 and 2017. The vertical and longitudinal displacements are estimated using multi-track Sentinel-1 SAR data and orientation angle of bridges, and we converted the displacements into thermal dilation and long-term vertical deflection. From COSMO-SkyMed data, we calculated the horizontal thermal dilation and long-term vertical deflection assuming that they dominantly contribute to the horizontal and vertical displacements, respectively. This assumption appeared reasonable based on the comparison with calculations from Sentinel-1 data. The deflection patterns exhibit downward movements at the mid-spans between towers. The results reveal that both bridges have been suffering long-term deflection over the observation period. Thus, this study verifies the potential to monitor the long-term deflection and implies that the bridges need to be monitored periodically.
Mean Shift Segmentation Assessment for Individual Forest Tree Delineation from Airborne Lidar Data
Authors: Xiao Wen , Zaforemska Aleksandra , Smigaj Magdalena , Wang Yunsheng , Gaulton Rachel
Published: May 2019

Airborne lidar has been widely used for forest characterization to facilitate forest ecological and management studies. With the availability of increasingly higher point density, individual tree delineation (ITD) from airborne lidar point clouds has become a popular yet challenging topic, due to the complexity and diversity of forests. One important step of ITD is segmentation, for which various methodologies have been studied. Among them, a long proven image segmentation method, mean shift, has been applied directly onto 3D points, and has shown promising results. However, there are variations among those who implemented the algorithm in terms of the kernel shape, adaptiveness and weighting. This paper provides a detailed assessment of the mean shift algorithm for the segmentation of airborne lidar data, and the effect of crown top detection upon the validation of segmentation results. The results from three different datasets revealed that a crown-shaped kernel consistently generates better results (up to 7 percent) than other variants, whereas weighting and adaptiveness do not warrant improvements.
A Highly Selective Turn-on Fluorescent Probe for the Detection of Aluminum and Its Application to Bio-Imaging
Authors: Wang Liguo , Yang Jing , Wang Huan , Ran Chongzhao , Su Ying , Zhao Long
Published: May 2019

Aluminum is the most abundant metallic element in the Earth’s crust and acts as a non-essential element for biological species. The accumulation of excessive amounts of aluminum can be harmful to biological species. Thus, the development of convenient and selective tools for the aluminum detection is necessary. In this work, a highly selective aluminum ion fluorescent probe N’-(2,5-dihydroxybenzylidene)acetohydrazide (Al-II) has been successfully synthesized and systemically characterized. The fluorescence intensity of this probe shows a significant enhancement in the presence of Al3+, which is subject to the strong quench effects caused by Cu2+ and Fe3+. The binding ratio of probe-Al3+ was determined from the Job’s plot to be 1:1. Moreover, the probe was demonstrated to be effective for in vivo imaging of the intracellular aluminum ion in both living Drosophila S2 cells and Malpighian tubules.
Detecting Malicious False Frame Injection Attacks on Surveillance Systems at the Edge Using Electrical Network Frequency Signals
Authors: Nagothu Deeraj , Chen Yu , Blasch Erik , Aved Alexander , Zhu Sencun
Published: May 2019

Over the past few years, the importance of video surveillance in securing national critical infrastructure has significantly increased, with applications including the detection of failures and anomalies. Accompanied by the proliferation of video is the increasing number of attacks against surveillance systems. Among the attacks, False Frame Injection (FFI) attacks that replay video frames from a previous recording to mask the live feed has the highest impact. While many attempts have been made to detect FFI frames using features from the video feeds, video analysis is computationally too intensive to be deployed on-site for real-time false frame detection. In this paper, we investigated the feasibility of FFI attacks on compromised surveillance systems at the edge and propose an effective technique to detect the injected false video and audio frames by monitoring the surveillance feed using the embedded Electrical Network Frequency (ENF) signals. An ENF operates at a nominal frequency of 60 Hz/50 Hz based on its geographical location and maintains a stable value across the entire power grid interconnection with minor fluctuations. For surveillance system video/audio recordings connected to the power grid, the ENF signals are embedded. The time-varying nature of the ENF component was used as a forensic application for authenticating the surveillance feed. The paper highlights the ENF signal collection from a power grid creating a reference database and ENF extraction from the recordings using conventional short-time Fourier Transform and spectrum detection for robust ENF signal analysis in the presence of noise and interference caused in different harmonics. The experimental results demonstrated the effectiveness of ENF signal detection and/or abnormalities for FFI attacks.