bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum Lipid, Amino Acid and Acylcarnitine Profiles of Obese Cats Supplemented with Dietary Choline and Fed to Maintenance Energy Requirements
Authors: Verbrugghe Adronie , Rankovic Alexandra , Armstrong Shafeeq , Santarossa Amanda , Kirby Gordon M. , Bakovic Marica
Published: July 2021

Obesity is a health concern for domestic cats. Obesity and severe energy restriction predispose cats to feline hepatic lipidosis. As choline is linked to lipid metabolism, we hypothesized that dietary choline supplementation would assist in reducing hepatic fat through increased lipoprotein transport and fatty acid oxidation. Twelve obese cats (body condition score [BCS] ≥ 8/9) were split into two groups. Cats were fed a control (n = 6; 4587 mg choline/kg dry matter [DM]) or a high choline diet (n = 6; 18,957 mg choline/kg DM) for 5 weeks, for adult maintenance. On days 0 and 35, fasted blood was collected, and the body composition was assessed. Serum lipoprotein and biochemistry profiles, plasma amino acids and plasma acylcarnitines were analyzed. The body weight, BCS and body composition were unaffected (p > 0.05). Choline increased the serum cholesterol, triacylglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol and plasma methionine (p < 0.05) and decreased the serum blood urea nitrogen and alkaline phosphatase (p < 0.05). Choline also reduced the plasma acylcarnitine to free carnitine ratio (p = 0.006). Choline may assist in eliminating hepatic fat through increased fat mobilization and enhanced methionine recycling.
Novel Genes Associated with Dairy Traits in Sarda Sheep
Authors: Pazzola Michele , Vacca Giuseppe Massimo , Paschino Pietro , Bittante Giovanni , Dettori Maria Luisa
Published: July 2021

The aim of the present research was to analyze the variability of 45 SNPs from different genes involved in metabolism and innate immunity to perform an association analysis with the milk yield, composition and milk coagulation traits. A population of 1112 Sarda breed sheep was sampled. Genotyping was generated by a TaqMan Open ArrayTM. Thirty out of the 45 SNPs were polymorphic, and 12 displayed a minor allele frequency higher than 0.05. An association analysis showed that the variability at genes PRKAG3 and CD14 was significantly associated with the daily milk yield. The variability at PRKAG3 was also associated with the protein and casein content, somatic cell score and bacterial score. The variation at the PRKAA2 gene was associated with the milk lactose concentration. The SNPs at CD14 were also associated with the traditional milk coagulation properties, while the SNPs at GHR and GHRHR were associated with kSR, a derived coagulation parameter related to the rate of syneresis. The information provided here is new and increases our knowledge of genotype–phenotype interactions in sheep. Our findings might be useful in appropriate breeding schemes to be set up for the Sarda sheep breed, but these should be confirmed by further studies, possibly performed on independent populations.
Clinical Outcomes and Safety of Meropenem–Colistin versus Meropenem–Tigecycline in Patients with Carbapenem-Resistant Acinetobacter baumannii Pneumonia
Authors: Park Jae-Min , Yang Kyung-Sook , Chung You-Seung , Lee Ki-Byung , Kim Jeong-Yeon , Kim Sun-Bean , Sohn Jang-Wook , Yoon Young-Kyung
Published: July 2021

This study compared the clinical outcomes and safety of meropenem–colistin versus meropenem–tigecycline in the treatment of adult patients with carbapenem-resistant Acinetobacter baumannii (CRAB) pneumonia. A retrospective observational study of patients with CRAB pneumonia was performed at a 1048-bed university-affiliated hospital in the Republic of Korea between June 2013 and January 2020. All adult patients initially treated with meropenem–colistin were compared with those treated with meropenem–tigecycline to evaluate in-hospital mortality and adverse events. Altogether, 66 patients prescribed meropenem–colistin and 24 patients prescribed meropenem–tigecycline were included. All patients had nosocomial pneumonia, and 31.1% had ventilator-associated pneumonia. The minimum inhibitory concentrations of meropenem ≤ 8 μg/mL and tigecycline ≤ 2 μg/mL were 20.0% and 81.1%, respectively. The in-hospital and 28-day mortality rates were 40% and 32%, respectively. In the Cox proportional hazard regression analysis, predictors associated with in-hospital mortality included procalcitonin ≥ 1 ng/mL (adjusted hazard ratio (aHR), 3.39; 95% confidence interval (CI) 1.40–8.19; p = 0.007) and meropenem–colistin combination therapy (aHR, 2.58; 95% CI, 1.07–6.23; p = 0.036). Episodes of nephrotoxicity were significantly more common in the meropenem–colistin group than in the meropenem–tigecycline group (51.5% vs. 12.5%, p = 0.001). Meropenem–tigecycline combination therapy might be a valuable treatment option for patients with CRAB pneumonia.
Antituberculosis Targeted Drug Delivery as a Potential Future Treatment Approach
Authors: Mazlan Mohd Khairul Nizam , Mohd Tazizi Mohammad Hafizie Dianel , Ahmad Rosliza , Noh Muhammad Amirul Asyraf , Bakhtiar Athirah , Wahab Habibah A. , Mohd Gazzali Amirah
Published: July 2021

Mycobacterium tuberculosis (Mtb) is the microorganism that causes tuberculosis. This infectious disease has been around for centuries, with the earliest record of Mtb around three million years ago. The discovery of the antituberculosis agents in the 20th century has managed to improve the recovery rate and reduce the death rate tremendously. However, the conventional antituberculosis therapy is complicated by the development of resistant strains and adverse drug reactions experienced by the patients. Research has been conducted continuously to discover new, safe, and effective antituberculosis drugs. In the last 50 years, only two molecules were approved despite laborious work and costly research. The repurposing of drugs is also being done with few drugs; antibiotics, particularly, were found to have antituberculosis activity. Besides the discovery work, enhancing the delivery of currently available antituberculosis drugs is also being researched. Targeted drug delivery may be a potentially useful approach to be developed into clinically accepted treatment modalities. Active targeting utilizes a specifically designed targeting agent to deliver a chemically conjugated drug(s) towards Mtb. Passive targeting is very widely explored, with the development of multiple types of nanoparticles from organic and inorganic materials. The nanoparticles will be engulfed by macrophages and this will eliminate the Mtb that is present in the macrophages, or the encapsulated drug may be released at the sites of infections that may be in the form of intra- and extrapulmonary tuberculosis. This article provided an overview on the history of tuberculosis and the currently available treatment options, followed by discussions on the discovery of new antituberculosis drugs and active and passive targeting approaches against Mycobacterium tuberculosis.
Cytoprotective Effect of Liposomal Puerarin on High Glucose-Induced Injury in Rat Mesangial Cells
Authors: Barro Lassina , Hsiao Jui-Ting , Chen Chu-Yin , Chang Yu-Lung , Hsieh Ming-Fa
Published: July 2021

In diabetic patients, high glucose and high oxidative states activate gene expression of transforming growth factor beta (TGF-β) and further translocate Smad proteins into the nucleus of renal cells. This signal pathway is characterized as the onset of diabetic nephropathy. Puerarin is an active ingredient extracted from Pueraria lobata as an anti-hyperglycemic and anti-oxidative agent. However, the poor oral availability and aqueous solubility limit its pharmaceutical applications. The present paper reports the liposomal puerarin and its protective effect on high glucose-injured rat mesangial cells (RMCs). The purity of puerarin extracted from the root of plant Pueraria lobata was 83.4% as determined by the high-performance liquid chromatography (HPLC) method. The liposomal puerarin was fabricated by membrane hydration followed by ultrasound dispersion and membrane extrusion (pore size of 200 nm). The fabricated liposomes were examined for the loading efficiency and contents of puerarin, the particle characterizations, the radical scavenge and the protective effect in rat mesangial cells, respectively. When the liposomes were subjected to 20 times of membrane extrusion, the particle size of liposomal puerarin can be reduced to less than 200 nm. When liposomal puerarin in RMCs in high glucose concentration (33 mM) was administered, the over-expression of TGF-β and the nuclear translocation of Smad 2/3 proteins was both inhibited. Therefore, this study successfully prepared the liposomal puerarin and showed the cytoprotective effect in RMCs under high glucose condition.
Melatonin as a Reducer of Neuro- and Vasculotoxic Oxidative Stress Induced by Homocysteine
Authors: Karolczak Kamil , Watala Cezary
Published: July 2021

The antioxidant properties of melatonin can be successfully used to reduce the effects of oxidative stress caused by homocysteine. The beneficial actions of melatonin are mainly due to its ability to inhibit the generation of the hydroxyl radical during the oxidation of homocysteine. Melatonin protects endothelial cells, neurons, and glia against the action of oxygen radicals generated by homocysteine and prevents the structural changes in cells that lead to impaired contractility of blood vessels and neuronal degeneration. It can be, therefore, assumed that the results obtained in experiments performed mainly in the in vitro models and occasionally in animal models may clear the way to clinical applications of melatonin in patients with hyperhomocysteinemia, who exhibit a higher risk of developing neurodegenerative diseases (e.g., Parkinson’s disease or Alzheimer’s disease) and cardiovascular diseases of atherothrombotic etiology. However, the results that have been obtained so far are scarce and have seldom been performed on advanced in vivo models. All findings predominately originate from the use of in vitro models and the scarcity of clinical evidence is huge. Thus, this mini-review should be considered as a summary of the outcomes of the initial research in the field concerning the use of melatonin as a possibly efficient attenuator of oxidative stress induced by homocysteine.
Ovalbumin and Kappa-Carrageenan Mixture Suppresses the Oxidative and Structural Changes in the Myofibrillar Proteins of Grass Carp (Ctenopharyngodon idella) during Frozen Storage
Authors: Walayat Noman , Wang Xiukang , Nawaz Asad , Zhang Zhongli , Abdullah  , Khalifa Ibrahim , Saleem Muhammad Hamzah , Mushtaq Bilal Sajid , Pateiro Mirian , Lorenzo José M. , Fiaz Sajid , Ali Shafaqat
Published: July 2021

This study was done to analyze the cryoprotective influence of ovalbumin (OVA) with kappa-carrageenan (KC) in grass carp myofibrillar proteins during frozen storage. Ca2+-ATPase activity of MP was significantly reduced due to protein denaturation and showed a direct association with decreased sulphydryl (SH) contents and tertiary structural properties. Besides that, an increase in carbonyl, surface hydrophobicity, and dityrosine contents was observed. The addition of OVA-KC significantly restricted the decline in Ca2+-ATPase and SH groups, which were further confirmed by the retarded increase in carbonyls. Furthermore, the addition of OVA-KC increased the stability of α-helix contents. Moreover, MP treated with 6% OVA-KC also improved intermolecular interaction forces linked with gelling and water holding properties of MP. Therefore, it can be concluded that OVA-KC could be used as an effective cryoprotectant in fish and related products for preservation and commercialization.
Innovative Investigation of Reflector Options for the Control of a Chloride-Based Molten Salt Zero-Power Reactor
Authors: Merk Bruno , Detkina Anna , Atkinson Seddon , Litskevich Dzianis , Cartland-Glover Gregory
Published: July 2021

Molten salt reactors have gained substantial interest in the last years due to their flexibility and their potential for simplified closed fuel cycle operations for massive net-zero energy production. However, a zero-power reactor experiment will be an essential first step into the process delivering this technology. The choice of the optimal reflector material is one of the key issues for such experiments since, on the one hand, it offers huge cost savings potential due to reduced fuel demand; on the other hand, an improper choice of the reflector material can have negative effects on the quality of the experiments. The choice of the reflector material is, for the first time, introduced through a literature review and a discussion of potential roles of the reflector. The 2D study of different potential reflector materials has delivered a first down-selection with SS304 as the representative for stainless steel, lead, copper, graphite, and beryllium oxide. A deeper look identified, in addition, iron-based material with a high Si content. The following evaluation of the power distribution has shown the strong influence of the moderating reflectors, creating a massively disturbed power distribution with a peak at the core boundary. This effect has been confirmed through a deeper analysis of the 2D multi-group flux distribution, which led to the exclusion of the BeO and the graphite reflector. The most promising materials identified were SS304, lead, and copper. The final 3D Monte Carlo study demonstrated that all three materials have the potential to reduce the required amount of fuel by up to 60% compared with NaCl, which has been used in previous studies and is now taken as the reference. An initial cost analysis has identified the SS304 reflector as the most attractive solution. The results of the 2D multi-group deterministic study and the 3D multi-group Monte Carlo study have been confirmed through a continuous energy Monte Carlo reference calculation, showing only minor differences.
The Reuse of Industrial By-Products for the Synthesis of Innovative Porous Materials, with the Aim to Improve Urban Air Quality
Authors: Cornelio Antonella , Zanoletti Alessandra , Braga Roberto , Depero Laura Eleonora , Bontempi Elza
Published: July 2021

This works concerns the characterization and the evaluation of adsorption capability of innovative porous materials synthesized by using alginates and different industrial by-products: silica fume and bottom ash. Hydrogen peroxide was used as pore former to generate a porosity able to trap particulate matter (PM). These new materials are compared with the reference recently proposed porous SUNSPACE hybrid material, which was obtained in a similar process, by using silica fume. Structural, morphological, colorimetric and porosimetric analyses were performed to evaluate the differences between the obtained SUNSPACE typologies. The sustainability of the proposed materials was evaluated in terms of the Embodied Energy and Carbon Footprint to quantify the benefits of industrial by-products reuse. Adsorption tests were also performed to compare the ability of samples to trap PM. For this aim, titania suspension, with particles size about 300 nm, was used to simulate PM in the nanoparticle range. The results show that the material realized with bottom ash has the best performance.
Shape and Weighting Optimization of a Subarray for an mm-Wave Phased Array Antenna
Authors: Jeong Taeyong , Yun Juho , Oh Kyunghyun , Kim Jihyung , Woo Dae Woong , Hwang Keum Cheol
Published: July 2021

This paper discusses how to optimize the weighting of individual subarrays to derive the low sidelobe level (SLL) based on quadratic programming (QP) and how to derive QP parameters to ensure that the objective function is composed of the quadratic function form, with the actual number identical to the standard objective function of QP. Next, in order to analyze the SLL, a 24 × 24 phased array antenna was compared with 96 transmit–receive modules (TRMs) attached only to the subarray stage and a phased array antenna with 576 TRMs attached to all radiating elements without a subarray. Optimized weighting was applied to the array antennas with a subarray, and Taylor weighting was applied to the array antennas without a subarray. The number of TRMs used in the phased array antenna with the optimized weighting was reduced by 83.3% compared to the phased array antenna in which TRMs were attached to all radiating elements. The SLL and the half-power beamwidths (HPBWs) of the two antennas were practically identical in a narrow beam-scanning environment. Finally, an array pattern (AP) in which mutual coupling between the radiating elements was considered was calculated to verify the optimized weighting. Moreover, the optimized weighting was applied to CST Microwave Studio (an EM full-wave simulation) to compare the results from the AP calculation and a simulation. It was confirmed that the two results above are largely indistinguishable. The analysis found that the HPBW is 3.6∘ × 3.6∘ and the SLL is −26.18 dB from AP calculations in the boresight direction. When each 5∘ beam was scanned at the azimuth and elevation, the corresponding HPBW values were 3.7∘ × 3.7∘ and 3.7∘ × 3.7∘ and the SLLs were −22.70 dB and −24.44 dB according to the AP calculations.
Scalability of Water Property Measurements in Space and Time on a Brackish Archipelago Coast
Authors: Nylén Tua , Tolvanen Harri , Suominen Tapio
Published: July 2021

Our paper aims at advancing global change management in marine archipelago environments. Water properties vary along temporal and vertical gradients, and studies indicate that these patterns may be site-specific, i.e., they may vary at local or regional scales. Understanding these complex processes is crucial for designing environmental monitoring campaigns or assessing the scalability of their results. To our knowledge, the four-dimensional (temporal, vertical and horizontal) patterns of water quality have not been statistically quantified. In this paper, we partition the variation in four key water property variables into temporal, vertical and horizontal dimensions, by utilising a unique pre-existing high-density dataset and multilevel regression modelling. The dataset comprised measurements of temperature, salinity, pH and chlorophyll-a concentration, sampled eight times from April to October on the SW Finnish archipelago coast. All variables were sampled along the depth gradient and at local (102 m) and regional scales (104 m) at 20 sites. All measured variables varied significantly along the temporal and vertical gradients, and the overall levels, temporal patterns and vertical gradients of these variables were significantly site-dependent. Our study confirms that many water properties, especially chlorophyll-a concentration, show high four-dimensional variability in the complex archipelago environment. Thus, studies on the regional dynamics of archipelago water properties call for a high sampling density in time, along the vertical gradient, and in space.
Arthroscope Localization in 3D Ultrasound Volumes Using Weakly Supervised Deep Learning
Authors: van der Burgt Jeroen M. A. , Camps Saskia M. , Antico Maria , Carneiro Gustavo , Fontanarosa Davide
Published: July 2021

This work presents an algorithm based on weak supervision to automatically localize an arthroscope on 3D ultrasound (US). The ultimate goal of this application is to combine 3D US with the 2D arthroscope view during knee arthroscopy, to provide the surgeon with a comprehensive view of the surgical site. The implemented algorithm consisted of a weakly supervised neural network, which was trained on 2D US images of different phantoms mimicking the imaging conditions during knee arthroscopy. Image-based classification was performed and the resulting class activation maps were used to localize the arthroscope. The localization performance was evaluated visually by three expert reviewers and by the calculation of objective metrics. Finally, the algorithm was also tested on a human cadaver knee. The algorithm achieved an average classification accuracy of 88.6% on phantom data and 83.3% on cadaver data. The localization of the arthroscope based on the class activation maps was correct in 92–100% of all true positive classifications for both phantom and cadaver data. These results are relevant because they show feasibility of automatic arthroscope localization in 3D US volumes, which is paramount to combining multiple image modalities that are available during knee arthroscopies.
Radon Levels of Water Sources in the Southwest Coastal Region of Peninsular Malaysia
Authors: Ismail Noor Fadilla , Hashim Suhairul , Sanusi Mohamad Syazwan Mohd , Abdul Rahman Ahmad Taufek , Bradley David Andrew
Published: July 2021

Across populations, the dominating source of public exposure to radiation is radon gas. In the present study, we aimed at determining the concentration of radon in water sources from the southwest coastal region of Peninsular Malaysia. A total of 27 water samples were taken from various water sources which included groundwater, as well as hot spring, lake, river, seawater, and tap water; the radon concentrations were measured using a RAD7 portable radon detector. The radon concentrations ranged from 0.07 ± 0.12 to 187 ± 12 Bq l−1, with an average of 21 ± 12 Bq l−1. The highest concentration was found in hot spring water, with an average concentration of 99 ± 6 Bq l−1, while the lowest concentration was found in tap water, with an average concentration of 1.95 ± 0.61 Bq l−1. The average concentrations of radon for all categories of sampled water were below the 100 Bq l−1 WHO guidance level for safe drinking water. According to the ICRP effective dose conversion factor and UNSCEAR (2000), the total effective dose from the summation of inhaled and imbibed water was calculated from the aqueous radon concentrations, with an average effective dose of 4.45 µSv y−1, well within the WHO safe drinking water guideline value of 100 µSv per year. The results of this study could support the efforts of authorities and regulators who are responsible for controlling and strategizing to ensure public safety against radon exposures.
Optimization of Computed Tomography Angiography Protocols for Follow-Up Type B Aortic Dissection Patients by Using 3D Printed Model
Authors: Wu Chia-An , Squelch Andrew , Jansen Shirley , Sun Zhonghua
Published: July 2021

Thoracic endovascular aortic repair (TEVAR) is a life-saving therapy for type B aortic dissection (TBAD). However, surveillance computed tomography (CT) scans in post-TEVAR patients are associated with high radiation dose, thus resulting in potential risk of radiation-induced malignancy. In this study, we developed a patient-specific three-dimensional (3D) printed phantom with stent grafts in situ, then scanned the phantom with different CT protocols to determine the optimal scanning parameters for post-treatment patients. The CT scans were conducted with different kVp and pitch values (80, 100, 120 kVp and pitch of 1.2, 1.5, 2.0, 2.5), resulting in a total of 12 datasets. Signal-to-noise ratio (SNR) was measured to determine and compare the image quality between different datasets. Results showed no significant differences in SNR between different kVp when the pitch value was 1.2. At low pitch values, a decrease in kVp from 120 to 80 led to a significant effective dose reduction by more than 20%. SNR decreased by 30% when pitch was increased from 1.2 to 2.5 at 80 kVp, and 20% at 120 kVp. In contrast, there was only a 3.9% decrease in SNR when kVp was reduced from 120 to 80 at pitch 1.2, and 15.9% at pitch 2.5. High pitch with 100 kVp can effectively reduce the dose while maintaining image quality.
Not Only Antimicrobial: Metronidazole Mitigates the Virulence of Proteus mirabilis Isolated from Macerated Diabetic Foot Ulcer
Authors: Khayyat Ahdab N. , Abbas Hisham A. , Mohamed Mamdouh F. A. , Asfour Hani Z. , Khayat Maan T. , Ibrahim Tarek S. , Youns Mahmoud , Khafagy El-Sayed , Abu Lila Amr S. , Safo Martin K. , Hegazy Wael A. H.
Published: July 2021

Diabetic foot ulcers are recognized to be a severe complication of diabetes, increasing the risk of amputation and death. The bacterial infection of Diabetic foot ulcers with virulent and resistant bacteria as Proteus mirabilis greatly worsens the wound and may not be treated with conventional therapeutics. Developing new approaches to target bacterial virulence can be helpful to conquer such infections. In the current work, we evaluated the anti-virulence activities of the widely used antibacterial metronidazole. The minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentrations (MEBC) were determined for selected antibiotics which P. mirabilis was resistant to them in the presence and absence of metronidazole in sub-MIC. The effect of metronidazole in sub-MIC on P. mirabilis virulence factors as production of exoenzymes, motilities, adhesion and biofilm formation, were evaluated. Furthermore, molecular docking of metronidazole into P. mirabilis adhesion and essential quorum sensing (QS) proteins, was performed. The results revealed a significant ability of metronidazole to in-vitro inhibit P. mirabilis virulence factors and antagonize its essential proteins. Moreover, metronidazole markedly decreased the MICs and MBECs of tested antibiotics. Conclusively, metronidazole in sub-MIC is a plausible anti-virulence and anti-QS agent that can be combined to other antibiotics as anti-virulence adjuvant to defeat aggressive infections.
Quantitative Assessment of Motor Function by an End-Effector Upper Limb Rehabilitation Robot Based on Admittance Control
Authors: Qian Chao , Li Wei , Jia Tianyu , Li Chong , Lin Ping-Ju , Yang Yiyong , Ji Linhong
Published: July 2021

Various rehabilitation robots have been developed to assist the movement training of the upper limbs of stroke patients, among which some have been used to evaluate the motor recovery. However, how to understand the recovery of motor function from the quantitative assessment following robot-assisted rehabilitation training is still not clear. The objective of this study is to propose a quantitative assessment method of motor function based on the force and trajectory characteristics during robotic training to reflect motor functional recovery. To assist stroke patients who are not able to move voluntarily, an assistive training mode was developed for the robot-assisted rehabilitation system based on admittance control. Then, to validate the relationship between characteristic information and functional recovery, a clinical experiment was conducted, in which nine stroke patients and nine healthy subjects were recruited. The results showed a significant difference in movement range and movement smoothness during trajectory tracking tasks between stroke patients and healthy subjects. The two parameters above have a correlation with the Fugl-Meyer Assessment for Upper Extremity (FMU) of the involved patients. The multiple linear regression analysis showed FMU was positively correlated with parameters (R2=0.91,p<0.005). This finding indicated that the above-mentioned method can achieve quantitative assessment of motor function for stroke patients during robot-assisted rehabilitation training, which can contribute to promoting rehabilitation robots in clinical practice.
Arabidopsis Disulfide Reductase, Trx-h2, Functions as an RNA Chaperone under Cold Stress
Authors: Lee Eun Seon , Park Joung Hun , Wi Seong Dong , Chae Ho Byoung , Paeng Seol Ki , Bae Su Bin , Phan Kieu Anh Thi , Lee Sang Yeol
Published: July 2021

The thioredoxin-h (Trx-h) family of Arabidopsis thaliana comprises cytosolic disulfide reductases. However, the physiological function of Trx-h2, which contains an additional 19 amino acids at its N-terminus, remains unclear. In this study, we investigated the molecular function of Trx-h2 both in vitro and in vivo and found that Arabidopsis Trx-h2 overexpression (Trx-h2OE) lines showed significantly longer roots than wild-type plants under cold stress. Therefore, we further investigated the role of Trx-h2 under cold stress. Our results revealed that Trx-h2 functions as an RNA chaperone by melting misfolded and non-functional RNAs, and by facilitating their correct folding into active forms with native conformation. We showed that Trx-h2 binds to and efficiently melts nucleic acids (ssDNA, dsDNA, and RNA), and facilitates the export of mRNAs from the nucleus to the cytoplasm under cold stress. Moreover, overexpression of Trx-h2 increased the survival rate of the cold-sensitive E. coli BX04 cells under low temperature. Thus, our data show that Trx-h2 performs function as an RNA chaperone under cold stress, thus increasing plant cold tolerance.
New Transition and Energy Levels of Three-Times Ionized Krypton (Kr IV)
Authors: Raineri M. , Gallardo M. , Reyna Almandos J. , Trigueiros A. G. , Pagan C. J. B.
Published: July 2021

A capillary pulsed-discharge and a theta-pinch were used to record Kr spectra in the region of 330–4800 Å. A set of 168 transitions of these spectra were classified for the first time. We extended the analysis to twenty-five new energy levels belonging to 3s23p24d, 3s23p25d even configurations. We calculated weighted transition probabilities (gA) for all of the experimentally observed lines and lifetimes for new energy levels using a relativistic Hartree–Fock method, including core-polarization effects.
Myofibroblasts: Function, Formation, and Scope of Molecular Therapies for Skin Fibrosis
Authors: Tai Yifan , Woods Emma L. , Dally Jordanna , Kong Deling , Steadman Robert , Moseley Ryan , Midgley Adam C.
Published: July 2021

Myofibroblasts are contractile, α-smooth muscle actin-positive cells with multiple roles in pathophysiological processes. Myofibroblasts mediate wound contractions, but their persistent presence in tissues is central to driving fibrosis, making them attractive cell targets for the development of therapeutic treatments. However, due to shared cellular markers with several other phenotypes, the specific targeting of myofibroblasts has long presented a scientific and clinical challenge. In recent years, myofibroblasts have drawn much attention among scientific research communities from multiple disciplines and specialisations. As further research uncovers the characterisations of myofibroblast formation, function, and regulation, the realisation of novel interventional routes for myofibroblasts within pathologies has emerged. The research community is approaching the means to finally target these cells, to prevent fibrosis, accelerate scarless wound healing, and attenuate associated disease-processes in clinical settings. This comprehensive review article describes the myofibroblast cell phenotype, their origins, and their diverse physiological and pathological functionality. Special attention has been given to mechanisms and molecular pathways governing myofibroblast differentiation, and updates in molecular interventions.
Candidate Genes Encoding Dopamine Receptors as Predictors of the Risk of Antipsychotic-Induced Parkinsonism and Tardive Dyskinesia in Schizophrenic Patients
Authors: Vaiman Elena E. , Shnayder Natalia A. , Novitsky Maxim A. , Dobrodeeva Vera S. , Goncharova Polina S. , Bochanova Elena N. , Sapronova Margarita R. , Popova Tatiana E. , Tappakhov Alexey A. , Nasyrova Regina F.
Published: July 2021

(1) Introduction: Extrapyramidal disorders form the so-called extrapyramidal syndrome (EPS), which is characterized by the occurrence of motor disorders as a result of damage to the basal ganglia and the subcortical-thalamic connections. Often, this syndrome develops while taking medications, in particular antipsychotics (APs). (2) Purpose: To review studies of candidate genes encoding dopamine receptors as genetic predictors of development of AP-induced parkinsonism (AIP) and AP-induced tardive dyskinesia (AITD) in patients with schizophrenia. (3) Materials and Methods: A search was carried out for publications of PubMed, Web of Science, Springer, and e-Library databases by keywords and their combinations over the last 10 years. In addition, the review includes earlier publications of historical interest. Despite extensive searches of these commonly used databases and search terms, it cannot be ruled out that some publications were possibly missed. (4) Results: The review considers candidate genes encoding dopamine receptors involved in pharmacodynamics, including genes DRD1, DRD2, DRD3, and DRD4. We analyzed 18 genome-wide studies examining 37 genetic variations, including single nucleotide variants (SNVs)/polymorphisms of four candidate genes involved in the development of AIP and AITD in patients with schizophrenia. Among such a set of obtained results, only 14 positive associations were revealed: rs1799732 (141CIns/Del), rs1800497 (C/T), rs6275 (C/T), rs6275 (C/T) DRD2; rs167771 (G/A) DRD3 with AIP and rs4532 (A/G) DRD1, rs6277 (C/T), rs6275 (C/T), rs1800497 (C/T), rs1079597 (A/G), rs1799732 (141CIns/Del), rs1045280 (C/G) DRD2, rs6280 (C/T), rs905568 (C/G) DRD3 with AITD. However, at present, it should be recognized that there is no final or unique decision on the leading role of any particular SNVs/polymorphisms in the development of AIP and AITD. (5) Conclusion: Disclosure of genetic predictors of the development of AIP and AITD, as the most common neurological adverse drug reactions (ADRs) in the treatment of patients with psychiatric disorders, may provide a key to the development of a strategy for personalized prevention and treatment of the considered complication of AP therapy for schizophrenia in real clinical practice.
High Na+ Salt Diet and Remodeling of Vascular Smooth Muscle and Endothelial Cells
Authors: Bkaily Ghassan , Simon Yanick , Jazzar Ashley , Najibeddine Houssein , Normand Alexandre , Jacques Danielle
Published: July 2021

Our knowledge on essential hypertension is vast, and its treatment is well known. Not all hypertensives are salt-sensitive. The available evidence suggests that even normotensive individuals are at high cardiovascular risk and lower survival rate, as blood pressure eventually rises later in life with a high salt diet. In addition, little is known about high sodium (Na+) salt diet-sensitive hypertension. There is no doubt that direct and indirect Na+ transporters, such as the Na/Ca exchanger and the Na/H exchanger, and the Na/K pump could be implicated in the development of high salt-induced hypertension in humans. These mechanisms could be involved following the destruction of the cell membrane glycocalyx and changes in vascular endothelial and smooth muscle cells membranes’ permeability and osmolarity. Thus, it is vital to determine the membrane and intracellular mechanisms implicated in this type of hypertension and its treatment.
Bioactive Compounds with Antiglioma Activity from Marine Species
Authors: Khotimchenko Rodion , Bryukhovetskiy Igor , Khotimchenko Maksim , Khotimchenko Yuri
Published: July 2021

The search for new chemical compounds with antitumor pharmacological activity is a necessary process for creating more effective drugs for each specific malignancy type. This review presents the outcomes of screening studies of natural compounds with high anti-glioma activity. Despite significant advances in cancer therapy, there are still some tumors currently considered completely incurable including brain gliomas. This review covers the main problems of the glioma chemotherapy including drug resistance, side effects of common anti-glioma drugs, and genetic diversity of brain tumors. The main emphasis is made on the characterization of natural compounds isolated from marine organisms because taxonomic diversity of organisms in seawaters significantly exceeds that of terrestrial species. Thus, we should expect greater chemical diversity of marine compounds and greater likelihood of finding effective molecules with antiglioma activity. The review covers at least 15 classes of organic compounds with their chemical formulas provided as well as semi-inhibitory concentrations, mechanisms of action, and pharmacokinetic profiles. In conclusion, the analysis of the taxonomic diversity of marine species containing bioactives with antiglioma activity is performed noting cytotoxicity indicators and to the tumor cells in comparison with similar indicators of antitumor agents approved for clinical use as antiglioblastoma chemotherapeutics.
Circulating MicroRNA-21 and MicroRNA-122 as Prognostic Biomarkers in Hepatocellular Carcinoma Patients Treated with Transarterial Chemoembolization
Authors: Pelizzaro Filippo , Cardin Romilda , Sartori Anna , Imondi Angela , Penzo Barbara , Aliberti Camillo , Ponzoni Alberto , Vitale Alessandro , Cillo Umberto , Farinati Fabio
Published: July 2021

Background: MicroRNAs (miRNAs) have been proposed as biomarkers in hepatocellular carcinoma (HCC). We aim at evaluating miR-21 and miR-122 in HCC patients treated with drug-eluting beads transarterial chemoembolization (DEB-TACE) as prognostic biomarkers and investigating their correlation with hypoxia inducible factor-1α (HIF-1α) serum levels. Methods: In this retrospective study, 12 healthy subjects, 28 cirrhotics, and 54 HCC patients (tested before and four weeks after DEB-TACE) were included. Whole blood miR-21 and miR-122 levels were measured by quantitative real time (qRT)-PCR, while serum HIF-1α was assessed by an enzyme-linked immunosorbent assay (ELISA) test. Results: The highest level of miR-21 was found in cirrhotics, while HCC patients had the highest level of miR-122 (which was even higher in “viral” HCC, p = 0.006). miR-21 ratio (after/before DEB-TACE) and miR-122 below their respective cut-offs identified patients with longer progression-free survival (p = 0.0002 and p = 0.02, respectively). The combined assessment of alpha-fetoprotein and miR-21 ratio, both independent prognostic predictors, identified early progressors among patients with complete or partial radiological response. miR-21 levels positively correlated with HIF-1α before (p = 0.045) and after DEB-TACE (p = 0.035). Conclusions: miR-21 ratio and miR-122 are useful prognostic markers after DEB-TACE. miR-21 correlates with HIF-1α and probably has a role in modulating angiogenesis in HCC.
The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics
Authors: Sfragano Patrick Severin , Moro Giulia , Polo Federico , Palchetti Ilaria
Published: July 2021

Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.
Neural Circuits, Microtubule Processing, Brain’s Electromagnetic Field—Components of Self-Awareness
Authors: Różyk-Myrta Alicja , Brodziak Andrzej , Muc-Wierzgoń Małgorzata
Published: July 2021

The known theories discussing the essence of consciousness have been recently updated. This prompts an attempt to integrate these explanations concerning several distinct components of the consciousness phenomenon such as the ego, and qualia perceptions. Therefore, it is useful to consider the latest publications on the ‘Orch OR’ and ‘cemi’ theories, which assume that quantum processing occurs in microtubules and that the brain’s endogenous electromagnetic field is important. The authors combine these explanations with their own theory describing the neural circuits realizing imagery. They try to present such an interdisciplinary, integrated theoretical model in a manner intuitively understandable to people with a typical medical education. In order to do this, they even refer to intuitively understandable metaphors. The authors maintain that an effective comprehension of consciousness is important for health care professionals because its disorders are frequent medical symptoms in emergencies, during general anesthesia and in the course of cognitive disorders in elderly people. The authors emphasize the current possibilities to verify these theses regarding the essence of consciousness thanks to the development of functional brain imaging methods—magnetoencephalography, transcranial magnetic stimulation—as well as clinical studies on the modification of perceptions and feelings by such techniques as mindfulness and the use of certain psychoactive substances, especially among people with self-awareness and identity disorders.
Impact of the COVID-19 Lockdown on a Long-Term Care Facility: The Role of Social Contact
Authors: Pereiro Arturo X. , Dosil-Díaz Carlos , Mouriz-Corbelle Romina , Pereira-Rodríguez Silvia , Nieto-Vieites Ana , Pinazo-Hernandis Sacramento , Pinazo-Clapés Carolina , Facal David
Published: July 2021

(1) Background: Long-term care facilities (LTCFs) have been harmed by the coronavirus, and older adults have remained isolated for a long time with many restrictions. The aim of this study was to measure the decline in cognitive, functional, and affective status in a care facility after the lockdown in the first wave of the COVID-19 pandemic and to compare it with previous measures in order to determine if this decline was accelerated. (2) Methods: Ninety-eight participants were recruited. Data from three retrospective pre-lockdown assessments and an additional post-lockdown assessment were analyzed. Mixed ANOVA analyses were performed according to the Clinical Dementia Rating levels, considering social-contact frequency during the lockdown as a covariate. (3) Results: The cognitive and functional scores were lower and depression scores were higher after the strict lockdown, accelerating a general pattern of decline that was already present in LTCF residents. The frequency of social contact eliminated the measurement differences in the cognitive and functional scores and the group differences in depression scores. (4) Conclusions: The effects of the SARS-CoV-2 lockdown in an LTCF were mediated by the frequency of contact. Clinical implications: Preventive measures must be taken to ensure social contact with relatives and friends and reduce the negative consequences of social isolation in LTCFs.
A Critical Success Factor Framework for Implementing Sustainable Innovative and Affordable Housing: A Systematic Review and Bibliometric Analysis
Authors: Moghayedi Alireza , Awuzie Bankole , Omotayo Temitope , Le Jeune Karen , Massyn Mark , Ekpo Christiana Okobi , Braune Manfred , Byron Paimaan
Published: July 2021

The actualization of affordable housing remains a challenge. This challenge is exacerbated by the increasing societal demand for the incorporation of sustainability principles into such housing types to improve levels of occupant health and well-being whilst avouching the desired levels of affordability. Innovative technologies and practices have been described as beneficial to the effectuation of sustainable affordable housing. However, knowledge concerning the deployment of innovative technologies and practices in sustainable affordable housing (sustainable, innovative, affordable housing—SIAH) delivery remains nascent. Consequently, there is a lack of a common ontology among stakeholders concerning how to realize SIAH. This study aims to contribute toward the development of this body of knowledge through the establishment of the critical success factors (CSFs) for effective SIAH implementation. To achieve this objective, a systematic review and bibliometric analysis focusing on a juxtaposition of sustainable, innovative and affordable housing concepts was carried out based on the relevant literature. This led to the identification and clustering of CSFs for these housing concepts at individual levels and as a collective (SIAH). The findings of the study consisted of the establishment of four distinct yet interrelated facets through which SIAH can be achieved holistically, namely, housing design, house element, housing production method and housing technology. A total of 127 CSFs were found to be aligned to these facets, subsequently clustered, and conclusively used for the development of a SIAH CSF framework. The most frequently occurring CSFs with predominant interconnections were the utilization of energy-efficient systems/fittings, tenure security, a comfortable and healthy indoor environment, affordable housing price in relation to income and using water-efficient systems/fittings CSFs, and establishing the emergent SIAH CSF framework. The framework in this study is useful in the documentation of SIAH features for construction projects and further studies into SIAH CSFs.
Mouse Models in Meningioma Research: A Systematic Review
Authors: Boetto Julien , Peyre Matthieu , Kalamarides Michel
Published: July 2021

Meningiomas are the most frequent primitive central nervous system tumors found in adults. Mouse models of cancer have been instrumental in understanding disease mechanisms and establishing preclinical drug testing. Various mouse models of meningioma have been developed over time, evolving in light of new discoveries in our comprehension of meningioma biology and with improvements in genetic engineering techniques. We reviewed all mouse models of meningioma described in the literature, including xenograft models (orthotopic or heterotopic) with human cell lines or patient derived tumors, and genetically engineered mouse models (GEMMs). Xenograft models provided useful tools for preclinical testing of a huge range of innovative drugs and therapeutic options, which are summarized in this review. GEMMs offer the possibility of mimicking human meningiomas at the histological, anatomical, and genetic level and have been invaluable in enabling tumorigenesis mechanisms, including initiation and progression, to be dissected. Currently, researchers have a range of different mouse models that can be used depending on the scientific question to be answered.
Missense Variants of Uncertain Significance: A Powerful Genetic Tool for Function Discovery with Clinical Implications
Authors: Sessa Gaetana , Ehlén Åsa , von Nicolai Catharina , Carreira Aura
Published: July 2021

The breast cancer susceptibility gene BRCA2 encodes a multifunctional protein required for the accurate repair of DNA double-strand breaks and replicative DNA lesions. In addition, BRCA2 exhibits emerging important roles in mitosis. As a result, mutations in BRCA2 may affect chromosomal integrity in multiple ways. However, many of the BRCA2 mutations found in breast cancer patients and their families are single amino acid substitutions, sometimes unique, and their relevance in cancer risk remains difficult to assess. In this review, we focus on three recent reports that investigated variants of uncertain significance (VUS) located in the N-terminal region of BRCA2. In this framework, we make the case for how the functional evaluation of VUS can be a powerful genetic tool not only for revealing novel aspects of BRCA2 function but also for re-evaluating cancer risk. We argue that other functions beyond homologous recombination deficiency or “BRCAness” may influence cancer risk. We hope our discussion will help the reader appreciate the potential of these functional studies in the prevention and diagnostics of inherited breast and ovarian cancer. Moreover, these novel aspects in BRCA2 function might help find new therapeutic strategies.