bluuebunny/crossref_metadata_2025 huggingface dataset:
A longitudinal study reveals persistence of antimicrobial resistance on livestock farms is not due to antimicrobial usage alone
Authors: Smith Richard P. , May Hannah E. , AbuOun Manal , Stubberfield Emma , Gilson Daniel , Chau Kevin K. , Crook Derrick W. , Shaw Liam P. , Read Daniel S. , Stoesser Nicole , Vilar Maria Jose , Anjum Muna F.
Published: March 2023

Introduction

There are concerns that antimicrobial usage (AMU) is driving an increase in multi-drug resistant (MDR) bacteria so treatment of microbial infections is becoming harder in humans and animals. The aim of this study was to evaluate factors, including usage, that affect antimicrobial resistance (AMR) on farm over time.

Methods

A population of 14 cattle, sheep and pig farms within a defined area of England were sampled three times over a year to collect data on AMR in faecal Enterobacterales flora; AMU; and husbandry or management practices. Ten pooled samples were collected at each visit, with each comprising of 10 pinches of fresh faeces. Up to 14 isolates per visit were whole genome sequenced to determine presence of AMR genes.

Results

Sheep farms had very low AMU in comparison to the other species and very few sheep isolates were genotypically resistant at any time point. AMR genes were detected persistently across pig farms at all visits, even on farms with low AMU, whereas AMR bacteria was consistently lower on cattle farms than pigs, even for those with comparably high AMU. MDR bacteria was also more commonly detected on pig farms than any other livestock species.

Discussion

The results may be explained by a complex combination of factors on pig farms including historic AMU; co-selection of AMR bacteria; variation in amounts of antimicrobials used between visits; potential persistence in environmental reservoirs of AMR bacteria; or importation of pigs with AMR microbiota from supplying farms. Pig farms may also be at increased risk of AMR due to the greater use of oral routes of group antimicrobial treatment, which were less targeted than cattle treatments; the latter mostly administered to individual animals. Also, farms which exhibited either increasing or decreasing trends of AMR across the study did not have corresponding trends in their AMU. Therefore, our results suggest that factors other than AMU on individual farms are important for persistence of AMR bacteria on farms, which may be operating at the farm and livestock species level.
Characterization of a lytic Pseudomonas aeruginosa phage vB_PaeP_ASP23 and functional analysis of its lysin LysASP and holin HolASP
Authors: Cui Jiaqi , Shi Xiaojie , Wang Xinwei , Sun Huzhi , Yan Yanxin , Zhao Feiyang , Zhang Can , Liu Wenhua , Zou Ling , Han Lei , Pan Qiang , Ren Huiying
Published: March 2023

In this study, we isolated a lytic Pseudomonas aeruginosa phage (vB_PaeP_ASP23) from the sewage of a mink farm, characterized its complete genome and analyzed the function of its putative lysin and holin. Morphological characterization and genome annotation showed that phage ASP23 belonged to the Krylovirinae family genus Phikmvvirus, and it had a latent period of 10 min and a burst size of 140 pfu/infected cell. In minks challenged with P. aeruginosa, phage ASP23 significantly reduced bacterial counts in the liver, lung, and blood. The whole-genome sequencing showed that its genome was a 42,735-bp linear and double-stranded DNA (dsDNA), with a G + C content of 62.15%. Its genome contained 54 predicted open reading frames (ORFs), 25 of which had known functions. The lysin of phage ASP23 (LysASP), in combination with EDTA, showed high lytic activity against P. aeruginosa L64. The holin of phage ASP23 was synthesized by M13 phage display technology, to produce recombinant phages (HolASP). Though HolASP exhibited a narrow lytic spectrum, it was effective against Staphylococcus aureus and Bacillus subtilis. However, these two bacteria were insensitive to LysASP. The findings highlight the potential of phage ASP23 to be used in the development of new antibacterial agents.
Highlighting the potential of Synechococcus elongatus PCC 7942 as platform to produce α-linolenic acid through an updated genome-scale metabolic modeling
Authors: Santos-Merino María , Gargantilla-Becerra Álvaro , de la Cruz Fernando , Nogales Juan
Published: March 2023

Cyanobacteria are prokaryotic organisms that capture energy from sunlight using oxygenic photosynthesis and transform CO2 into products of interest such as fatty acids. Synechococcus elongatus PCC 7942 is a model cyanobacterium efficiently engineered to accumulate high levels of omega-3 fatty acids. However, its exploitation as a microbial cell factory requires a better knowledge of its metabolism, which can be approached by using systems biology tools. To fulfill this objective, we worked out an updated, more comprehensive, and functional genome-scale model of this freshwater cyanobacterium, which was termed iMS837. The model includes 837 genes, 887 reactions, and 801 metabolites. When compared with previous models of S. elongatus PCC 7942, iMS837 is more complete in key physiological and biotechnologically relevant metabolic hubs, such as fatty acid biosynthesis, oxidative phosphorylation, photosynthesis, and transport, among others. iMS837 shows high accuracy when predicting growth performance and gene essentiality. The validated model was further used as a test-bed for the assessment of suitable metabolic engineering strategies, yielding superior production of non-native omega-3 fatty acids such as α-linolenic acid (ALA). As previously reported, the computational analysis demonstrated that fabF overexpression is a feasible metabolic target to increase ALA production, whereas deletion and overexpression of fabH cannot be used for this purpose. Flux scanning based on enforced objective flux, a strain-design algorithm, allowed us to identify not only previously known gene overexpression targets that improve fatty acid synthesis, such as Acetyl-CoA carboxylase and β-ketoacyl-ACP synthase I, but also novel potential targets that might lead to higher ALA yields. Systematic sampling of the metabolic space contained in iMS837 identified a set of ten additional knockout metabolic targets that resulted in higher ALA productions. In silico simulations under photomixotrophic conditions with acetate or glucose as a carbon source boosted ALA production levels, indicating that photomixotrophic nutritional regimens could be potentially exploited in vivo to improve fatty acid production in cyanobacteria. Overall, we show that iMS837 is a powerful computational platform that proposes new metabolic engineering strategies to produce biotechnologically relevant compounds, using S. elongatus PCC 7942 as non-conventional microbial cell factory.
Trichoderma asperellum empowers tomato plants and suppresses Fusarium oxysporum through priming responses
Authors: Sehim Amira E. , Hewedy Omar A. , Altammar Khadijah A. , Alhumaidi Maryam S. , Abd Elghaffar Rasha Y.
Published: March 2023

Plant-associated microbes play crucial roles in plant health and promote growth under stress. Tomato (Solanum lycopersicum) is one of the strategic crops grown throughout Egypt and is a widely grown vegetable worldwide. However, plant disease severely affects tomato production. The post-harvest disease (Fusarium wilt disease) affects food security globally, especially in the tomato fields. Thus, an alternative effective and economical biological treatment to the disease was recently established using Trichoderma asperellum. However, the role of rhizosphere microbiota in the resistance of tomato plants against soil-borne Fusarium wilt disease (FWD) remains unclear. In the current study, a dual culture assay of T. asperellum against various phytopathogens (e.g., Fusarium oxysporum, F. solani, Alternaria alternata, Rhizoctonia solani, and F. graminerarum) was performed in vitro. Interestingly, T. asperellum exhibited the highest mycelial inhibition rate (53.24%) against F. oxysporum. In addition, 30% free cell filtrate of T. asperellum inhibited F. oxysporum by 59.39%. Various underlying mechanisms were studied to explore the antifungal activity against F. oxysporum, such as chitinase activity, analysis of bioactive compounds by gas chromatography–mass spectrometry (GC–MS), and assessment of fungal secondary metabolites against F. oxysporum mycotoxins in tomato fruits. Additionally, the plant growth-promoting traits of T. asperellum were studied (e.g., IAA production, Phosphate solubilization), and the impact on tomato seeds germination. Scanning electron microscopy, plant root sections, and confocal microscopy were used to show the mobility of the fungal endophyte activity to promote tomato root growth compared with untreated tomato root. T. asperellum enhanced the growth of tomato seeds and controlled the wilt disease caused by the phytopathogen F. oxysporum by enhancing the number of leaves as well as shoot and root length (cm) and fresh and dry weights (g). Furthermore, Trichoderma extract protects tomato fruits from post-harvest infection by F. oxysporum. Taking together, T. asperellum represents a safe and effective controlling agent against Fusarium infection of tomato plants.
Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study
Authors: Patry Alice , Bothorel Philippe , Labrunie Anaïs , Renesme Laurent , Lehours Philippe , Benard Melinda , Dubois Damien , Ponthier Laure , Meyer Sylvain , Norbert Karine , Villeneuve Laurent , Jouvencel Philippe , Leysenne David , Chainier Delphine , Luce Sandrine , Grélaud Carole , Ploy Marie-Cecile , Bedu Antoine , Garnier Fabien
Published: March 2023

Objectives

The study aimed to describe the dynamics and risk factors of Gram-negative bacteria (GNB) acquisition in preterm infants.

Methods

This prospective multicenter French study included mothers hospitalized for preterm delivery and their newborns, followed until hospital discharge. Maternal feces and vaginal fluids at delivery, and neonatal feces from birth to discharge were tested for cultivable GNB, potential acquired resistance, and integrons. The primary outcome was the acquisition of GNB and integrons in neonatal feces, and their dynamics, evaluated by survival analysis using the actuarial method. Risk factors were analyzed using Cox models.

Results

Two hundred thirty-eight evaluable preterm dyads were included by five different centers over 16 months. GNB were isolated in 32.6% of vaginal samples, with 15.4% of strains producing extended-spectrum beta-lactamase (ESBL) or hyperproducing cephalosporinase (HCase), and in 96.2% of maternal feces, with 7.8% ESBL-GNB or HCase-GNB. Integrons were detected in 40.2% of feces and 10.6% of GNB strains. The mean (SD) length of stay of newborns was 39.5 (15.9) days; 4 died in the hospital. At least one infection episode occurred in 36.1% of newborns. The acquisition of GNB and integrons was progressive from birth to discharge. At discharge, half of newborns had ESBL-GNB or HCase-GNB, independently favored by a premature rupture of membranes (Hazard Ratio (HR), 3.41, 95% confidence interval (CI), 1.71; 6.81), and 25.6% had integrons (protective factor: multiple gestation, HR, 0.367, 95% CI, 0.195; 0.693).

Conclusion

In preterm newborns, the acquisitions of GNB, including resistant ones, and integrons are progressive from birth to discharge. A premature rupture of membranes favored the colonization by ESBL-GNB or Hcase-GNB.
Antibiotics affected the bacterial community structure and diversity in pore water and sediments with cultivated Phragmites australis in a typical Chinese shallow lake
Authors: Zhang Ling , Bai Junhong , Zhai Yujia , Zhang Kegang , Wei Zhuoqun , Wang Yaqi , Liu Haizhu , Xiao Rong , Jorquera Milko A.
Published: March 2023

The migration of antibiotics and bacterial communities between sediments and pore water occurring in the lake, which is affected by aquatic vegetation. However, the differences in bacterial community structure and biodiversity between pore water and sediments with plants in lakes under antibiotic stress are still poorly understood. We collected pore water and sediments in both wild and cultivated Phragmites australis regions in the Zaozhadian (ZZD) Lake to explore the characteristics of the bacterial community. Our results showed that the diversity of bacterial community in sediment samples were significantly higher than those in pore water samples in both P. australis regions. Due to higher antibiotic levels in sediments from the cultivated P. australis region, the composition of bacterial communities showed a difference, which reduced the relative abundance of dominant phyla in pore water and increased that in sediments. The higher bacterial variations in pore water could be explained by sediment in the cultivated P. australis region than that in wild P. australis region, therefore plant cultivation might change the source-sink pattern between sediments and pore water. The dominant factors shaping the bacterial communities in the wild P. australis region pore water or sediment were NH4-N, NO3-N, and particle size, while cultivated P. australis region pore water or sediment were oxytetracycline, tetracycline, etc. The findings of this work indicates that the antibiotic pollution caused by planting activities has a greater impact on the bacterial community, which will provide a reference for the use and management of antibiotics in lake ecosystems.
Isolation of brightness induction effects on target patches from adjacent surrounds and remote backgrounds
Authors: Blakeslee Barbara , McCourt Mark E.
Published: March 2023

The brightness (perceived intensity) of a region of visual space depends on its luminance and on the luminance of nearby regions. This phenomenon is called brightness induction and includes both brightness contrast and assimilation. Historically, and on a purely descriptive level, brightness contrast refers to a directional shift in target brightness away from the brightness of an adjacent region while assimilation refers to a brightness shift toward that of an adjacent region. In order to understand mechanisms, it is important to differentiate the descriptive terms contrast and assimilation from the optical and/or neural processes, often similarly named, which cause the effects. Experiment 1 isolated the effect on target patch (64 cd/m2) matching luminance (brightness) of six surround-ring widths (0.1°–24.5°) varied over 11 surround-ring luminances (32–96 cd/m2). Using the same observers, Experiment 2 examined the effect of the identical surround-ring parameters on target patch matching luminance in the presence of a dark (0.0 cd/m2) and a bright (96 cd/m2) remote background. By differencing the results of Experiment 1 (the isolated effect of the surround-ring) from those of Experiment 2 (the combined effect of the surround-ring with the dark and bright remote background) we further isolated the effect of the remote background. The results reveal that surround-rings and remote backgrounds produce brightness contrast effects in the target patch that are of the same or opposite polarity depending on the luminance polarity of these regions relative to target patch luminance. The strength of brightness contrast from the surround-ring varied with surround-ring luminance and width. Brightness contrast (darkening) in the target from the bright remote background was relatively constant in magnitude across all surround-ring luminances and increased in magnitude with decreasing surround-ring width. Brightness contrast (brightening) from the isolated dark remote background also increased in magnitude with decreasing surround-ring width: however, despite some regional flattening of the functions due to the fixed luminance of the dark remote background, induction magnitude was much reduced in the presence of a surround-ring of greater luminance than the target patch indicating a non-linear interaction between the dark remote background and surround-ring luminance.
Neural pathways from hypothalamic orexin neurons to the ventrolateral preoptic area mediate sleep impairments induced by conditioned fear
Authors: Ma Caifen , Zhou Ning , Ma Kang , Niu Jiandong , Mi Ting , He Zhenquan , Wen Yujun , Liu Chunhong , He Zhongyi , Niu Jianguo
Published: March 2023

Introduction

Fear and sleep impairments common co-exist, but the underlying mechanisms remain unclear. Hypothalamic orexinergic neurons are involved in the regulation of sleep-wake and fear expression. The ventrolateral preoptic area (VLPO) is an essential brain region to promote sleep, and orexinergic axonal fibers projecting to the VLPO are involved in the maintenance of sleep-wake. Neural pathways from hypothalamic orexin neurons to the VLPO might mediate sleep impairments induced by conditioned fear.

Methods

To verify above hypothesis, electroencephalogram (EEG) and electromyogram (EMG) were recorded for analysis of sleep-wake states before and 24 h after conditioned fear training. The retrograde tracing technique and immunofluorescence staining was used to identify the projections from the hypothalamic orexin neurons to the VLPO and to observe their activation in mice with conditioned fear. Moreover, optogenetic activation or inhibition of hypothalamic orexin-VLPO pathways was performed to observe whether the sleep-wake can be regulated in mice with conditioned fear. Finally, orexin-A and orexin receptor antagonist was administered into the VLPO to certify the function of hypothalamic orexin-VLPO pathways on mediating sleep impairments induced by conditioned fear.

Results

It was found that there was a significant decrease in the non-rapid eye movement (NREM) and rapid eye movement (REM) sleep time and a significant increase in the wakefulness time in mice with conditioned fear. The results of retrograde tracing technique and immunofluorescence staining showed that hypothalamic orexin neurons projected to the VLPO and observed the CTB labeled orexin neurons were significantly activated (c-Fos+) in the hypothalamus in mice with conditioned fear. Optogenetic activation of hypothalamic orexin to the VLPO neural pathways significantly decreased NREM and REM sleep time and increased wakefulness time in mice with conditioned fear. A significant decrease in NREM and REM sleep time and an increase in wakefulness time were observed after the injection of orexin-A into the VLPO, and the effects of orexin-A in the VLPO were blocked by a pre-administrated dual orexin antagonist (DORA).

Conclusion

These findings suggest that the neural pathways from hypothalamic orexinergic neurons to the VLPO mediate sleep impairments induced by conditioned fear.
Interaction between the intestinal flora and the severity of diversion colitis after low anterior resection of rectal cancer
Authors: Sun Qiang , Shi Yunjie , Liang Xiaoben , Lu Hao , Huang Yu , Zhu Lin , Wang Wenqiang , Zhang Wei , Hu Zhiqian , Li Xinxing
Published: March 2023

Background

Diversion colitis (DC) is nonspecific inflammation of the distal intestinal mucosa following disruption of colonic continuity with colonic dysfunction. The colonscopic score is a good tool for differentiating the severity of patients with DC. At present, no studies have analyzed the pathogenesis of DC from the perspective of the diversity and and differences of intestinal flora.

Methods

Retrospective study: Clinical information were collected from patients with low rectal cancer admitted to the Department of Anorectal Surgery, Changzheng Hospital, from April 2017 to April 2019. These patients underwent laparoscopic low anterior resection (LAR) combined with terminal ileum enterostomy (dual-chamber). We used chi-square test to comparethe clinical baseline information, clinical symptoms, and colonscopic characteristics between different severity of DC. Propsective oberservational study: We recruited 40 patients with laparoscopic anterior low resection combined with terminal ileum enterostomy and they were further classified into mild group and severe group according to the scores of colonscopic examinations for DC. 16s-rDNA sequencing was carried out to analyze the diversity and and differences of intestinal flora in the intestinal lavage fluid of the two groups.

Results

In retrospective study, we found that age, BMI, history of diabetes, and symptoms associated with the stoma state were the independent risk factors that affect DC severity (P<0.05). Meanwhile, age, BMI, history of diabetes and colonscopic score were found to be independent risk factors affecting the severity of diarrhea after ileostomy closure surgery(P<0.05), which was consistent with our results of differentiating the severity of DC under endoscopy; In propsective oberservational study, 40 patients with low rectal cancer recruited by sample size calculation, 23 were in the mild group and 17 in the severe group. The results of 16s-rDNA sequencing showed that intestinal flora with high enrichment values primarily consisted of Bifidobacteriales and Prevotella in mild group, whereas that in the severe group consisted of Providencia and Dorea. The functional predictions on such two types of intestinal flora were mainly focused on lipid synthesis, glycan synthesis, metabolism, and amino acid metabolism pathways.

Conclusion

After ileostomy closure surgery, a series of severe clinical symptoms might appear in DC patients. There are significant differences in local and systemic inflammatory responses, composition of intestinal flora between DC patients with different colonscopic scores, which provide a basis for the clinical interventional treatment for DC in patients with permanent stoma.
Effect of exercise on bone health in children and adolescents with cancer during and after oncological treatment: A systematic review and meta-analysis
Authors: Marmol-Perez Andres , Ubago-Guisado Esther , Rodriguez-Solana Andrea , Gil-Cosano Jose J. , Martinez-Vizcaino Vicente , Cavero-Redondo Ivan , Ruiz Jonatan R. , Gracia-Marco Luis
Published: March 2023

Background: Although regular physical activity and exercise programs might improve bone health caused by oncological treatment and the disease itself, it remains unknown the pooled effect of exercise interventions following frequency, intensity, time and type prescriptions.

Objective: This systematic review and meta-analysis aimed to synthesise evidence regarding the effectiveness of exercise interventions on bone health in children and adolescents with cancer during and after oncological treatment.

Methods: A systematic search was conducted in the MEDLINE (via PubMed), Web of Science and Scopus databases from November 2021 to January 2022. Randomised controlled trials (RCTs) and non-RCTs reporting pre-post changes of the effectiveness of exercise interventions on DXA-measured bone parameters in young population (1–19 years) during or after oncological treatment were included. Pooled (ESs) and 95% confidence intervals (95%CIs) were calculated. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed.

Results: A total of eight trials with 341 participants were included. The meta-analyses did not reveal a statistically significant increase in whole body areal bone mineral density (ES = 0.10; 95%CI: −0.14, 0.34), lumbar spine (ES = 0.03; 95%CI: −0.21, 0.26) or femoral neck (ES = 0.10; 95%CI: −0.37, 0.56). Similarly, during the oncological treatment phase the ES was 0.04 (95%CI: −0.17, 0.25) and after the ES was 0.07 (95%CI: −0.20, 0.33).

Conclusion: To date, exercise interventions have been inappropriate and therefore, ineffective to illustrate any beneficial effect on bone health in children and adolescents with cancer during and after oncological treatment.

Systematic Review Registration: PROSPERO registration number: CRD42022310876
The impact of loneliness and social adaptation on depressive symptoms: Behavioral and brain measures evidence from a brain health perspective
Authors: Franco-O´Byrne Daniel , Gonzalez-Gomez Raul , Morales Sepúlveda Juan Pablo , Vergara Mayte , Ibañez Agustin , Huepe David
Published: March 2023

Introduction

Early detection of depression is a cost-effective way to prevent adverse outcomes on brain physiology, cognition, and health. Here we propose that loneliness and social adaptation are key factors that can anticipate depressive symptoms.

Methods

We analyzed data from two separate samples to evaluate the associations between loneliness, social adaptation, depressive symptoms, and their neural correlates.

Results

For both samples, hierarchical regression models on self-reported data showed that loneliness and social adaptation have negative and positive effects on depressive symptoms. Moreover, social adaptation reduces the impact of loneliness on depressive symptoms. Structural connectivity analysis showed that depressive symptoms, loneliness, and social adaptation share a common neural substrate. Furthermore, functional connectivity analysis demonstrated that only social adaptation was associated with connectivity in parietal areas.

Discussion

Altogether, our results suggest that loneliness is a strong risk factor for depressive symptoms while social adaptation acts as a buffer against the ill effects of loneliness. At the neuroanatomical level, loneliness and depression may affect the integrity of white matter structures known to be associated to emotion dysregulation and cognitive impairment. On the other hand, socio-adaptive processes may protect against the harmful effects of loneliness and depression. Structural and functional correlates of social adaptation could indicate a protective role through long and short-term effects, respectively. These findings may aid approaches to preserve brain healthviasocial participation and adaptive social behavior.
Demographic variables, anthropometric indices, sleep quality, Metabolic Equivalent Task (MET), and developing diabetes in the southwest of Iran
Authors: Hosseini Seyed Ahmad , Beiranvand Samira , Zarea Kourosh , Noemani Kourosh
Published: March 2023

Propose

The present study has sought to investigate the prevalence of diabetes and its related risk factors, to examine the relationship between demographic variables, anthropometric indices, sleep quality, and Metabolic Equivalent Task (MET) with diabetes in Khuzestan province, southwest Iran.

Methods

The present study has a cross-sectional design (the baseline data of the Hoveyzeh cohort study as a sub-branch of the Persian Prospective Cohort Study). Comprehensive information from 10,009 adults (aged 35–70 years) was collected from May 2016 to August 2018 through a multi-part general questionnaire containing general characteristics, marital status, education, smoking, sleep quality, MET, and anthropometric indices. Data analysis was performed by SPSS software version 19.

Results

The mean age of the sample was 52.97 ± 8.99 years. 60.3% of the population were women and 67.7% were illiterate. Out of the 10,009 people surveyed, 1,733 stated that they have diabetes (17%). In 1,711 patients (17%) the amount of FBS was ≥126 mg/dl. There is a statistically significant relationship between diabetes and MET. More than 40% had BMI above 30. Anthropometric indices in diabetic and non-diabetic individuals were different. Also, there was a statistically significant difference between the mean duration of sleep and the use of sleeping pills in diabetic and non-diabetic groups (p < 0.05). Based on logistic regression, marital status [OR = 1.69 (95% CI, 1.24, 2.30)], education level [OR = 1.49 (95% CI, 1.22, 1.83)], MET [OR = 2.30 (95% CI, 2.01, 2.63)], height [OR = 0.99 (95% CI, 0.98, 0.99)], weight [OR = 1.007 (95% CI, 1.006, 1.012)], wrist circumference [OR = 1.10 (95% CI, 1.06, 1.14)], waist circumference [OR = 1.03 (95% CI, 1.02, 1.03)], waist-to-hip ratio [OR = 3.41 (95% CI, 2.70, 4.29)], and BMI [OR = 2.55 (95% CI, 1.53, 4.25)], are good predictors for diabetes.

Conclusion

The results of this study showed that the prevalence of diabetes in Hoveyzeh city, Khuzestan, Iran, was almost high. and emphasize that preventive interventions should focus on risk factors, especially socioeconomic status, and anthropometric indicators along with lifestyle.
Path analysis and empirical test of medical service enhancement for common prosperity under government participation
Authors: Chen Baoqi , Zhang Xigang
Published: March 2023

The level of medical services is the link between the two strategic goals of “healthy China” and “common prosperity,” and government participation plays an important adjustment role in it, so it is of great theoretical and practical significance to study its inner logic. In this paper, we firstly analyze the mechanism of medical service level to promote the development of common prosperity and the role of government in it; secondly, we construct a panel dynamic regression model and a threshold regression model to verify the relationship between the three. It is found that the contribution of both equity and efficiency of health care services to the achievement of common wealth is non-linear, and the degree of government participation plays an important adjustment role, with single and double threshold effects between them and the level of common prosperity, respectively. In the process of participating in the medical service market, the government should clearly position itself, actively play the demand-led role of the market, encourage private capital to provide quality medical services, and purposefully optimize the financial expenditure structure according to the local actual situation. There are many ways in which the government can be involved in health care, and there will be differences between China and other countries around the world. These are all worthy of further discussion.
The RNA-Seq data analysis shows how the ontogenesis defines aging
Authors: Salnikov Lev , Goldberg Saveli , Rijhwani Heena , Shi Yuran , Pinsky Eugene
Published: March 2023

This paper presents a global statistical analysis of the RNA-Seq results of the entire Mus musculus genome. We explain aging by a gradual redistribution of limited resources between two major tasks of the organism: its self-sustenance based on the function of the housekeeping gene group (HG) and functional differentiation provided by the integrative gene group (IntG). All known disorders associated with aging are the result of a deficiency in the repair processes provided by the cellular infrastructure. Understanding exactly how this deficiency arises is our primary goal. Analysis of RNA production data of 35,630 genes, from which 5,101 were identified as HG genes, showed that RNA production levels in the HG and IntG genes had statistically significant differences (p-value <0.0001) throughout the entire observation period. In the reproductive period of life, which has the lowest actual mortality risk for Mus musculus, changes in the age dynamics of RNA production occur. The statistically significant dynamics of the decrease of RNA production in the HG group in contrast to the IntG group was determined (p-value = 0.0045). The trend toward significant shift in the HG/IntG ratio occurs after the end of the reproductive period, coinciding with the beginning of the mortality rate increase in Mus musculus indirectly supports our hypothesis. The results demonstrate a different orientation of the impact of ontogenesis regulatory mechanisms on the groups of genes representing cell infrastructures and their organismal functions, making the chosen direction promising for further research and understanding the mechanisms of aging.
The role of bowel for minimally invasive treatment of stricture disease
Authors: Kronstedt Shane , Kaldany Alain , Patel Hiren V. , Elsamra Sammy E.
Published: March 2023

The management of urinary tract stricture disease has evolved over the last several decades, with robotic surgery representing a bourgeoning method for urologic reconstruction. Conventionally, proximal and mid-ureteral strictures, as well as lengthy urethral strictures, have presented a challenge for surgeons to create tension-free repairs. Options for repair include endoscopic dilation, endopyelotomy, ureteroplasty or pyeloplasty, and urethroplasty. Small and large bowel can be incorporated into various urinary tract stricture repairs. Their use has proven successful in reconstructing both upper and lower urinary tract strictures and offers flexibility for complex cases such as lengthy or multifocal strictures. While the use of bowel, most notably the appendix, for stricture repair is not a novel concept, a growing body of evidence supports its use with minimally invasive robotic approaches. In addition, there has been a substantial amount of recent data suggesting low rates of postoperative complications and long progression-free survival after robotic stricture repair using small bowel or rectum. We present a comprehensive review of literature outlining the role of the small bowel, appendix, and rectum in the minimally invasive repair of urinary tract stricture disease, as well as a description of the various techniques employed.
Solar activities associated with 3He-rich solar energetic particle events observed by Solar Orbiter
Authors: Nitta Nariaki V. , Bučík Radoslav , Mason Glenn M. , Ho George C. , Cohen Christina M. S. , Gómez-Herrero Raúl , Wang Linghua , Balmaceda Laura A.
Published: March 2023

A series of 3He-rich solar energetic particle (SEP) events was observed by Solar Orbiter in May 2021 at a radial distance of 0.95 AU. An isolated active region AR 12824 was likely the ultimate source of these SEP events. The period of the enhanced flux of 3He was also a period of frequent type III bursts in the decametric-hectometric range, confirming their close relationship. As in past studies, we try to find the solar activities possibly responsible for 3He-rich SEP events, using the type III bursts close to the particle injection times estimated from the velocity dispersion. But this exercise is not as straightforward as in many of the past studies since the region produced many more type III bursts and jet-like eruptions than the SEP injections. We may generalize the solar activities for the 3He-rich SEP events in question as coronal jets, but their appearances do not necessarily conform to classic jets that consist of a footpoint and a spire. Conversely, such jets often did not accompany type III bursts. The areas that produced jet-like eruptions changed within the active region from the first to the second set of 3He-rich SEP events, which may be related to the extended coronal mass ejection that launched stealthily.
A novel 3D-printed laryngoscope with integrated working channels for laryngeal surgery
Authors: Kienle Linus L. , Schild Leon R. , Böhm Felix , Grässlin Rene , Greve Jens , Hoffmann Thomas K. , Schuler Patrick J.
Published: March 2023

Background

For the surgical treatment of early-stage laryngeal cancer, the use of transoral laser microsurgery (TLM) has emerged as the gold standard. However, this procedure requires a straight line of sight to the operating field. Therefore, the patient's neck needs to be brought into a hyperextended position. In a considerable number of patients, this is not possible due to anomalies in the cervical spine anatomy or soft tissue scarring, e.g., after radiation. In these cases, adequate visualization of relevant laryngeal structures cannot be ensured using a conventional rigid operating laryngoscope, which may negatively affect the outcome of these patients.

Methods

We present a system based on a 3D-printed prototype of a curved laryngoscope with three integrated working channels (sMAC). The curved profile of the sMAC-laryngoscope is specifically adapted to the nonlinear anatomy of the upper airway structures. The central working channel provides access for flexible video endoscope imaging of the operating field while the two remaining channels provide access for flexible instrumentation. In a user study (n = 11), visualization and reachability of relevant laryngeal landmarks as well as the feasibility of basic surgical procedures with the proposed system were examined in a patient simulator. In a second setup, the system was evaluated for its applicability in a human body donor.

Results

All participants of the user study were able to visualize, reach and manipulate the relevant laryngeal landmarks. Reaching those took significantly less time in the second attempt compared to the first one (27.5 s ± 5.2 s vs. 39.7 s ± 16.5 s, p = 0.008) indicating a significant learning curve for handling the system. Instrument changes were performed quickly and reliably by all participants (10.9 s ± 1.7 s). All participants were able to bring the bimanual instruments into position for a vocal fold incision. Relevant laryngeal landmarks could be visualized and reached in the human body donor setup.

Conclusion

Possibly, the proposed system may develop into an alternative treatment option for patients with early-stage laryngeal cancer and restricted mobility of the cervical spine in the future. Further improvements of the system could include finer end effectors and a flexible instrument with a laser cutting tool.
Nano-adjuvanted dry powder vaccine for the mucosal immunization against airways pathogens
Authors: Canelli Elena , Ferrari Luca , Borghetti Paolo , Candela Francesco , Abiakam Nkemjika Sopuru , Bianchera Annalisa , Buttini Francesca , Magi Gian Enrico , Sonvico Fabio , Martelli Paolo , Bettini Ruggero
Published: March 2023

Nasal vaccination has been shown to provide optimal protection against respiratory pathogens. However, mucosal vaccination requires the implementation of specific immunization strategies to improve its effectiveness. Nanotechnology appears a key approach to improve the effectiveness of mucosal vaccines, since several nanomaterials provide mucoadhesion, enhance mucosal permeability, control antigen release and possess adjuvant properties. Mycoplasma hyopneumoniae is the main causative agent of enzootic pneumonia in pigs, a respiratory disease responsible for considerable economic losses in the pig farming worldwide. The present work developed, characterized, and tested in vivo an innovative dry powder nasal vaccine, obtained from the deposition on a solid carrier of an inactivated antigen and a chitosan-coated nanoemulsion, as an adjuvant. The nanoemulsion was obtained through a low-energy emulsification technique, a method that allowed to achieve nano droplets in the order of 200 nm. The oil phase selected was alpha-tocopherol, sunflower oil, and poly(ethylene glycol) hydroxystearate used as non-ionic tensioactive. The aqueous phase contained chitosan, which provides a positive charge to the emulsion, conferring mucoadhesive properties and favoring interactions with inactivated M. hyopneumoniae. Finally, the nanoemulsion was layered with a mild and scalable process onto a suitable solid carrier (i.e., lactose, mannitol, or calcium carbonate) to be transformed into a solid dosage form for administration as dry powder. In the experimental study, the nasal vaccine formulation with calcium carbonate was administered to piglets and compared to intramuscular administration of a commercial vaccine and of the dry powder without antigen, aimed at evaluating the ability of IN vaccination to elicit an in vivo local immune response and a systemic immune response. Intranasal vaccination was characterized by a significantly higher immune response in the nasal mucosa at 7 days post-vaccination, elicited comparable levels of Mycoplasma-specific IFN-γ secreting cells and comparable, if not higher, responsiveness of B cells expressing IgA and IgG in peripheral blood mononuclear cells, with those detected upon a conventional intramuscular immunization. In conclusion, this study illustrates a simple and effective strategy for the development of a dry powder vaccine formulation for nasal administration which could be used as alternative to current parenteral commercial vaccines.
In-Plane Libration Suppression of a Two-Segment Tethered Towing System
Authors: Chen Shouxu , Chen Weidong , Chen Ti , Kang Junjie
Published: March 2023

A tethered towing system provides an effective method for capturing pieces of space debris and dragging them out of orbit. This paper focuses on the in-plane stability analysis and libration control of a two-segment tethered towing system. The first segment is the same as the traditional single-tether towing system. The second segment is similar to a simplified space tether net. The dynamic equations are established in the orbit frame. Considering the elasticity of the tethers, the equilibrium solutions are obtained and the stability of equilibrium solutions is proved. An in-plane libration controller based on the sliding mode control scheme is designed to ensure the safety of the towing mission and save fuel. The controller suppressed the librations of the in-plane angles in the desired state by applying two external torques. Finally, simulation results are provided to validate the effectiveness of the proposed controller.
Structured Expert Judgment Elicitation in Conceptual Aircraft Design
Authors: Todorov Vladislav T. , Rakov Dmitry , Bardenhagen Andreas
Published: March 2023

Disruptive technologies and novel aircraft generations represent a potential approach to address the ambitious emission reduction goals in aviation. However, the introduction of innovative concepts is a time-consuming process, which might not necessarily yield an optimal design for a given flight mission and within the defined time frame. In order to address the need for a structured and more exhaustive search for novel concept generations, the Advanced Morphological Approach (AMA) and its further enhancement was introduced earlier. It implies the decomposition of design problems into functional attributes and appropriate technological alternatives. Subsequently, these are evaluated and combined into solutions, which are then projected onto a solution space. The current paper focuses on the technology evaluation step by deriving and integrating structured expert judgment elicitation (SEJE) techniques into conceptual aircraft design with the AMA. For this purpose, the first aim of the work is to justify the developed method by giving an overview and discussing the most prominent SEJE methods and their applications in aerospace. Then, the derived SEJE concept is described and applied in the form of an expert workshop on the use case of wing morphing architecture. As a result, a solution space of wing morphing architecture configurations is generated and analyzed. The workshop conduction and the expert feedback serve as valuable findings for both the further AMA enhancement and similar research.
Phytochemical Constituents of Indonesian Adlay (Coix lacrima-jobi L.) and Their Potential as Antioxidants and Crop Protection Agents
Authors: Andriana Yusuf , Fajriani Nabila Ayunisa , Iwansyah Ade Chandra , Xuan Tran Dang
Published: March 2023

Adlay (Coix lacryma-jobi L.) is a cereal crop that has traditionally been used for medicinal purposes. It is processed into nutritious food in China and Southeast Asian countries. This study assesses the phytochemical constituents of this plant and their potential as antioxidants and crop protection agents. The methanolic extracts from seeds of Indonesian adlay (C. lacryma-jobi) varieties including Agrotis, Ma-yuen, and Aquatic, were tested against 2,2-diphnyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to determine their free radical scavenging activity. The relationship between extraction solvents, phytochemical composition, and antioxidant activity was analyzed statistically using principal component analysis (PCA) to classify them based on the similarities among the components. The potential use of the phytochemicals as crop protection agents was also explored through a review of the literature. The Agrotis variety demonstrated the highest antioxidant activities (IC50 DPPH = 741.49 and ABTS =152.69 µg/mL). The ethyl acetate fraction of this variety showed the greatest antioxidant activity (IC50 DPPH and ABTS = 106.34 and 17.62 µg/mL, respectively), total phenolic content (275.16 mg GAE/g extract), and flavonoid content (37.41 mg QE/g extract). Fatty acids (FAs) and fatty acid methyl esters (FAMEs) accounted for 47.71 ± 0.02 and 41.73 ± 0.04%, respectively, and they were the major components of the extracts. The principal component analysis (PCA) revealed three different groups of phytochemical components in the seeds of Agrotis variety, including fatty acid methyl esters (FAMEs), such as methyl linoleate, methyl stearate, methyl vaccinates, and methyl palmitate, and fatty acids (FAs), including 7-hexadecanoid acid, bovinic acid, and 15-hydroxipentadecanoic acid. The final phytochemical group consisted of minor components, including uncategorized compounds such as decamethyl-tetrasiloxane and cycloalkenes. This study highlights the fact that C. lacrima-jobi is a promising source of natural antioxidants and agrochemicals.
First Description of the Underwater Sounds in the Mediterranean Monk Seal Monachus monachus in Greece: Towards Establishing a Vocal Repertoire
Authors: Charrier Isabelle , Huetz Chloé , Prevost Léa , Dendrinos Panagiotis , Karamanlidis Alexandros A.
Published: March 2023

The Mediterranean monk seal Monachus monachus is one of the most endangered pinnipeds in the world, and is classified as “Endangered” by the International Union for the Conservation of Nature. Any additional knowledge about the species is invaluable to its effective conservation. In the present study, we deployed an autonomous underwater recorder in an important reproductive area of the Mediterranean monk seal in Greece to describe its underwater vocal repertoire. Over the 330 h of continuous recordings, 9231 vocalizations were labelled as potentially produced by Mediterranean monk seals, and 1694 good quality calls were analyzed. We defined 18 call types divided into three main call categories: harmonic, noisy, and pulsative calls. We also described the soundscape in which this endangered species lives and found that human activities around the two main pupping caves had a strong impact on the sonic environment of these seals: the noise level produced by boat traffic was high, and occurred on an hourly (25 to 50 min/hour) and daily basis (10.8 to 16.9 h/day). Such high levels of noise might not only impair the communication of the species, but also impact its survival, as chronic noise can induce physiological stress.
A Comparison of Faulty Antenna Detection Methodologies in Planar Array
Authors: Boopalan Navaamsini , Ramasamy Agileswari K. , Nagi Farrukh
Published: March 2023

Broadcasting, radar, sonar and space telecommunication systems use phased arrays to produce directed signals to be transmitted at the desired angle. This system requires a large number of antenna elements. The presence of faulty element(s) in an array causes asymmetry, which results in a deformed radiation pattern with higher sidelobe levels. Higher sidelobe levels indicate waste of energy by transmitting and receiving signals in unwanted directions. Hence, it is important to develop a method that detects faulty elements and corrects the radiation pattern. To correct the failed radiation pattern, failed elements in an array must be identified first. There have been various studies conducted on linear array failed radiation pattern correction and the finding of faulty elements, but investigation on the planar array is limited. Further, the optimization suggested for linear arrays does not necessarily work for the planar array. In this study, planar array faulty antenna detection was developed with pattern search (PS), simulated annealing (SA), and particle swarm optimization (PSO) methods by reducing the Signal to Noise Ratio (SNR) as the objective function. The analysis was varied for 8 × 8 and 6 × 6 planar arrays with different types of failures. The results were compared to find the best method to identify the faulty element’s location in a planar array. The pattern search method produced outstanding results in finding the faulty element’s locations by providing 100% accuracy for all types of failure, while other methods failed to do the same.