bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Epidemiology and Resistance Trends of Common Urinary Pathogens in a Tertiary-Care Hospital: A 10-Year Surveillance Study
Authors: Gajdács Márió , Ábrók Marianna , Lázár Andrea , Burián Katalin
Published: July 2019

Background and Objective: Urinary tract infections (UTIs) are common in human medicine, affecting large patient populations worldwide. The principal cause of UTIs is uropathogenic Escherichia coli (UPEC) and Klebsiella, both in community and nosocomial settings. The assessment of local data on prevalence and resistance is essential to evaluate trends over time and to reflect on the national situation, compared to international data, using the methods of analytical epidemiology. Materials and Methods: The aim of this study was to assess resistance trends and epidemiology of UTIs caused by E. coli and Klebsiella species in inpatients and outpatients at a tertiary-care hospital in Hungary, using microbiological data. To evaluate resistance trends, several antibiotics were chosen as indicator drugs, based on local utilization data. Results: E. coli was the most prevalent isolate, representing 56.75 ± 4.86% for outpatients and 42.29 ± 2.94% for inpatients. For E. coli, the ratio of resistant strains for several antibiotics was significantly higher in the inpatient group, while in Klebsiella, similar trends were only observed for gentamicin. Extended-spectrum β-lactamase (ESBL)-producing isolates were detected in 4.33–9.15% and 23.22–34.22% from outpatient, 8.85–38.97% and 10.89–36.06% from inpatient samples for E. coli and Klebsiella, respectively. Conclusions: Resistance developments in common UTI pathogens (especially to fosfomycin, sulfamethoxazole-trimethoprim, fluoroquinolones, and 3rd generation cephalosporins), seriously curb therapeutic options, especially in outpatient settings.
Role of Autophagy on Heavy Metal-Induced Renal Damage and the Protective Effects of Curcumin in Autophagy and Kidney Preservation
Authors: Avila-Rojas Sabino Hazael , Lira-León Alejandra , Aparicio-Trejo Omar Emiliano , Reyes-Fermín Laura María , Pedraza-Chaverri José
Published: July 2019

Curcumin is a hydrophobic polyphenol compound extracted from the rhizome of turmeric. The protective effect of curcumin on kidney damage in multiple experimental models has been widely described. Its protective effect is mainly associated with its antioxidant and anti-inflammatory properties, as well as with mitochondrial function maintenance. On the other hand, occupational or environmental exposure to heavy metals is a serious public health problem. For a long time, heavy metals-induced nephrotoxicity was mainly associated with reactive oxygen species overproduction and loss of endogenous antioxidant activity. However, recent studies have shown that in addition to oxidative stress, heavy metals also suppress the autophagy flux, enhancing cell damage. Thus, natural compounds with the ability to modulate and restore autophagy flux represent a promising new therapeutic strategy. Furthermore, it has been reported in other renal damage models that curcumin’s nephroprotective effects are related to its ability to regulate autophagic flow. The data indicate that curcumin modulates autophagy by classic signaling pathways (suppression of protein kinase B (Akt)/mammalian target of rapamycin (mTOR) and/or by stimulation of adenosine monophosphate-activated protein kinase (AMPK) and extracellular signal-dependent kinase (ERK) pathways). Moreover, it allows lysosomal function preservation, which is crucial for the later stage of autophagy. However, future studies of autophagy modulation by curcumin in heavy metals-induced autophagy flux impairment are still needed.
Processing Variants in Medium-Mn Steels
Authors: Speer John , Rana Radhakanta , Matlock David , Glover Alexandra , Thomas Grant , De Moor Emmanuel
Published: July 2019

This paper highlights some recent efforts to extend the use of medium-Mn steels for applications other than intercritically batch-annealed steels with exceptional ductility (and strengths in the range of about 1000 MPa). These steels are shown to enable a range of promising properties. In hot-stamping application concepts, elevated Mn concentration helps to stabilize austenite and to provide a range of attractive property combinations, and also reduces the processing temperatures and likely eliminates the need for press quenching. The “double soaking” concept also provides a wide range of attractive mechanical property combinations that may be applicable in cold-forming applications, and could be implemented in continuous annealing and/or continuous galvanizing processes where Zn-coating would typically represent an additional austempering step. Quenching and partitioning of steels with elevated Mn concentrations have exhibited very high strengths, with attractive tensile ductility; and medium-Mn steels have been successfully designed for quenching and partitioning using room temperature as the quench temperature, thereby effectively decoupling the quenching and partitioning steps.
Crystal Chemistry and Properties of Elpidite and Its Ag-Exchanged Forms
Authors: Zubkova Natalia V. , Nikolova Rositsa P. , Chukanov Nikita V. , Kostov-Kytin Vladislav V. , Pekov Igor V. , Varlamov Dmitry A. , Larikova Tatiana S. , Kazheva Olga N. , Chervonnaya Nadezhda A. , Shilov Gennadiy V. , Pushcharovsky Dmitry Yu.
Published: July 2019

Elpidite from the Lovozero alkaline complex, Kola Peninsula, Russia, and Ag-exchanged forms of elpidite from two different localities (Lovozero and Khan Bogdo, Mongolia) were studied by means of single-crystal X-ray diffraction, electron microprobe analysis, thermogravimetry and IR spectroscopy. All studied samples retain the heteropolyhedral framework consisting of double Si6O15 chains (ribbons) and isolated ZrO6 octahedra. Zeolitic cavities in the initial elpidite from Lovozero (space group Pbm2, a = 14.6127(7), b = 7.3383(4), c = 7.1148(3) Å, V = 762.94(6) Å3) are occupied by Na+ cations and H2O molecules. Both Ag-exchanged forms are characterized by evident distortions of the heteropolyhedral framework and a strongly disordered arrangement of extra-framework cations which results in the appearance of the 14-14-14 Å unit cell (a = 14.1755(7), b = 14.6306(9), c = 14.2896(7) Å, V = 2963.6(3) Å3 for the Ag-exchanged form of elpidite from Lovozero and a = 14.1411(5), b = 14.5948(4), c = 14.3035(5) Å, V = 2952.04(17) Å3 for the Ag-exchanged form of elpidite from Khan Bogdo) and space group Cmce. Elpidite from both localities demonstrates a high exchange capacity to Ag. Exchanged Ag+ cations preferably occupy the sites that are close to the Na sites in the initial elpidite. The paper also contains a review of crystal chemical data on elpidite and its laboratory-modified forms.
Anti-Multiple Myeloma Potential of Secondary Metabolites from Hibiscus sabdariffa
Authors: Malacrida Alessio , Cavalloro Valeria , Martino Emanuela , Cassetti Arianna , Nicolini Gabriella , Rigolio Roberta , Cavaletti Guido , Mannucci Barbara , Vasile Francesca , Giacomo Marcello Di , Collina Simona , Miloso Mariarosaria
Published: July 2019

Multiple myeloma (MM) belongs to hematological cancers and its incidence is increasing worldwide. Despite recent advances in its therapy, MM still causes many deaths every year. In fact, current therapies sometimes fail and are associated with severe adverse effects, including neurotoxicity. As a part of our ongoing efforts to discover new potential therapies against MM, we prepared Hibiscus sabdariffa extracts obtained by a microwave-assisted solvent extraction and investigate their activity by in vitro assays on the RPMI-8226 cell line. The bioguided fractionation of the crude ethanolic extract allowed the identification of HsFC as the most effective extract. We assessed cell viability (MTT and Tripan blue test), cell migration (Boyden chamber assay), and neurotoxicity (DRG neurotoxicity assay). The promising results prompted us to further fractionate HsFC and we obtained two molecules effective against RPMI-8226 cells without neurotoxic effects at their active concentrations. Moreover, both compounds are able to significantly reduce cell migration.
Hybrid Solid-Phase Extraction for Selective Determination of Methamphetamine and Amphetamine in Dyed Hair by Using Gas Chromatography–Mass Spectrometry
Authors: Kwon Nam Hee , Lee Yu Rim , Kim Hee Seung , Cheong Jae Chul , Kim Jin Young
Published: July 2019

Sample preparation is an important step in the isolation of target compounds from complex matrices to perform their reliable and accurate analysis. Hair samples are commonly pulverized or processed as fine cut, depending on preference, before extraction by techniques such as solid-phase extraction (SPE), liquid–liquid extraction, and other methods. In this study, a method based on hybrid solid-phase extraction (hybridSPE) and gas chromatography–mass spectrometry (GC–MS) was developed and validated for the determination of methamphetamine (MA) and amphetamine (AP) in hair. The hair samples were mechanically pulverized after washing with de-ionized water and acetone. The samples were then sonicated in methanol at 50 °C for 1 h and centrifuged at 50,000× g for 3 min. The supernatants were transferred onto the hybridSPE cartridge and extracted using 1 mL of 0.05 M methanolic hydrogen chloride. The combined solutions were evaporated to dryness, derivatized using pentafluoropropionic anhydride, and analyzed by GC–MS. Excellent linearity (R2 > 0.9998) was achieved in the ranges of 0.05–5.0 ng/mg for AP and 0.1–10.0 ng/mg for MA. The recovery was 83.4–96.8%. The intra- and inter-day accuracies were −9.4% to 5.5% and −5.1% to 3.1%, while the intra- and inter-day precisions were within 8.3% and 6.7%, respectively. The limits of detections were 0.016 ng/mg for AP and 0.031 ng/mg for MA. The validated hybridSPE method was applied to dyed hair for MA and AP extraction and compared to a methanol extraction method currently being used in our laboratory. The results showed that an additional hybridSPE step improved the recovery by 5.7% for low-concentration quality control (QC) samples and by 24.1% for high-concentration QC samples. Additionally, the hybridSPE method was compared to polymeric reversed-phase SPE methods, and the absolute recoveries for hybridSPE were 50% and 20% greater for AP (1.5 ng/mg) and MA (3.0 ng/mg), respectively. In short, the hybridSPE technique was shown to minimize the matrix effects, improving GC–MS analysis of hair. Based on the results, the proposed method proved to be effective for the selective determination of MA and AP in hair samples.
An Established HPLC-MS/MS Method for Evaluation of the Influence of Salt Processing on Pharmacokinetics of Six Compounds in Cuscutae Semen
Authors: Liu Jiao , Zou Shuhan , Liu Wei , Li Jin , Wang Hui , Hao Jiao , He Jun , Gao Xiumei , Liu Erwei , Chang Yanxu
Published: July 2019

A sensitive and effective method was developed for clarifying the pharmacokinetic properties of six compounds (including hyperin, chlorogenic acid, neochlorogenic acid, p-coumaric acid, astragalin, and isoquercitrin) in two processed Cuscutae Semen samples by high performance liquid chromatography mass spectrometry (HPLC-MS/MS). The six compounds were separated by acetonitrile and 0.1% formic acid-water on an Agilent Eclipse plus C18 column (4.6 mm × 100 mm, 1.8 μm). All compounds were analyzed with negative ion mode in multiple reaction monitoring (MRM). The lower limits of quantification (LLOQ) of hyperin, astragalin, neochlorogenic acid, chlorogenic acid, isoquercitrin, and p-coumaric acid were 1, 0.1, 4, 0.1, 2, and 4 ng·mL−1, respectively. The validated approach was effectively used for the pharmacokinetics of six compounds of two processed Cuscutae Semen samples after oral administration to rat. The results indicated that salt processing could improve the adsorption and bioavailability of astragalin in Cuscutae Semen.
A Promising Polymer Blend Electrolytes Based on Chitosan: Methyl Cellulose for EDLC Application with High Specific Capacitance and Energy Density
Authors: Aziz Shujahadeen B. , Hamsan M. H. , Abdullah Ranjdar M. , Kadir M. F. Z.
Published: July 2019

In the present work, promising proton conducting solid polymer blend electrolytes (SPBEs) composed of chitosan (CS) and methylcellulose (MC) were prepared for electrochemical double-layer capacitor (EDLC) application with a high specific capacitance and energy density. The change in intensity and the broad nature of the XRD pattern of doped samples compared to pure CS:MC system evidencedthe amorphous character of the electrolyte samples. The morphology of the samples in FESEM images supported the amorphous behavior of the solid electrolyte films. The results of impedance and Bode plotindicate that the bulk resistance decreasedwith increasing salt concentration. The highest DC conductivity was found to be 2.81 × 10−3 S/cm. The electrical equivalent circuit (EEC) model was conducted for selected samples to explain the complete picture of the electrical properties.The performance of EDLC cells was examined at room temperature by electrochemical techniques, such as impedance spectroscopy, cyclic voltammetry (CV) and constant current charge–discharge techniques. It was found that the studied samples exhibit a very good performance as electrolyte for EDLC applications. Ions were found to be the dominant charge carriers in the polymer electrolyte. The ion transference number (tion) was found to be 0.84 while 0.16 for electron transference number (tel). Through investigation of linear sweep voltammetry (LSV), the CS:MC:NH4SCN system was found to be electrochemically stable up to 1.8 V. The CV plot revealed no redox peak, indicating the occurrence of charge double-layer at the surface of activated carbon electrodes. Specific capacitance (Cspe) for the fabricated EDLC was calculated using CV plot and charge–discharge analyses. It was found to be 66.3 F g−1 and 69.9 F g−1 (at thefirst cycle), respectively. Equivalent series resistance (Resr) of the EDLC was also identified, ranging from 50.0 to 150.0 Ω. Finally, energy density (Ed) was stabilized to anaverage of 8.63 Wh kg−1 from the 10th cycle to the 100th cycle. The first cycle obtained power density (Pd) of 1666.6 W kg−1 and then itdropped to 747.0 W kg−1 at the 50th cycle and continued to drop to 555.5 W kg−1 as the EDLC completed 100 cycles.
Marylosides A-G, Norcycloartane Glycosides from Leaves of Cymbidium Great Flower ‘Marylaurencin’
Authors: Yoneyama Tatsuro , Iseki Kanako , Noji Masaaki , Imagawa Hiroshi , Hashimoto Toshihiro , Kawano Sachiko , Baba Masaki , Kashiwada Yoshiki , Yahagi Tadahiro , Matsuzaki Keiichi , Umeyama Akemi
Published: July 2019

Seven novel norcycloartane glycosides, maryloside A–G (1–7), were isolated from the leaves of Cymbidium Great Flower ‘Marylaurencin’, along with a known norcycloartane glycoside, cymbidoside (8). These structures were determined on the basis of mainly NMR experiments as well as chemical degradation and X-ray crystallographic analysis. The isolated compounds (1–6 and 8) were evaluated for the inhibitory activity on lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-stimulated nitric oxide (NO) production in RAW 264.7 cells. Consequently, 1 and 3 exhibited moderate activity.
Similar Safety Profile of the Enantiomeric N-Aminoalkyl Derivatives of Trans-2-Aminocyclohexan-1-ol Demonstrating Anticonvulsant Activity
Authors: Słoczyńska Karolina , Koczurkiewicz Paulina , Piska Kamil , Powroźnik Beata , Wójcik-Pszczoła Katarzyna , Klaś Katarzyna , Wyszkowska-Kolatko Magdalena , Pękala Elżbieta
Published: July 2019

Epilepsy is one of the most common neurological disorder in the world. Many antiepileptic drugs cause multiple adverse effects. Moreover, multidrug resistance is a serious problem in epilepsy treatment. In the present study we evaluated the safety profile of three (1–3) new chiral N-aminoalkyl derivatives of trans-2-aminocyclohexan-1-ol demonstrating anticonvulsant activity. Our aim was also to determine differences between the enantiomeric compounds with respect to their safety profile. The results of the study indicated that compounds 1–3 are non-cytotoxic for astrocytes, although they exhibit cytotoxic activity against human glioblastoma cells. Moreover, 1–3 did not affect the viability of HepG2 cells and did not produce adducts with glutathione. Compounds 1–3 demonstrated no mutagenic activity either in the Salmonella typhimurium or in Vibrio harveyi tests. Additionally, the compounds displayed a strong or moderate antimutagenic effect. Finally, the P-glycoprotein (P-gp) ATPase assay demonstrated that both enantiomers are potent P-gp inhibitors. To sum up, our results indicate that the newly synthesized derivatives may be considered promising candidates for further research on anticonvulsant drug discovery and development. Our study indicated the similar safety profile of the enantiomeric N-aminoalkyl derivatives of trans-2-aminocyclohexan-1-ol, although in the previous studies both enantiomers differ in their biotransformation pathways and pharmacological activity.
Ionic Liquid-Microwave-Based Extraction of Biflavonoids from Selaginella sinensis
Authors: Li Dan , Sun Chengxin , Yang Jiaqiang , Ma Xiankui , Jiang Yongmei , Qiu Shaoliang , Wang Gang
Published: July 2019

Selaginella sinensis (Desv.) Spring has been used for many years as traditional Chinese medicine (TCM) for many years. Recently, ionic liquids (ILs) have attracted great attentions in extraction and separation technology of TCM as a new green solvent. In this paper, microwave assisted extraction-IL (MAE-IL) that extracted amentoflavone (AME) and hinokiflavone (HIN) from Selaginella sinensis was reported for the first time. The contents of two biflavonoids were simultaneously determined by a high performance liquid chromatography (HPLC) method. After different ionic liquids were compared, it was found [C6mim]BF4 had a high selectivity and efficiency. Moreover, the important extraction conditions, including solid-liquid ratio, IL concentration, extraction time, microwave power and radiation temperature, were also investigated and optimized by response surface methodology (RSM) using AME and HIN yields as index. The results showed that the extraction yields of AME and HIN from S. sinensis were 1.96 mg/g and 0.79 mg/g, respectively, under the optimal process parameters (0.55 mmol/L, 300 W, 40 min, 1:11 g/mL and 48 °C). Compared with the conventional extraction methods, MAE-IL could not only achieve higher yield in shorter time, but also could reduce the consumption of solvent. This effective, rapid and green MAE-IL method was suitable for the extraction of AME and HIN.
Quinoa Secondary Metabolites and Their Biological Activities or Functions
Authors: Lin Minyi , Han Peipei , Li Yuying , Wang Weixuan , Lai Daowan , Zhou Ligang
Published: July 2019

Quinoa (Chenopodium quinoa Willd.) was known as the “golden grain” by the native Andean people in South America, and has been a source of valuable food over thousands of years. It can produce a variety of secondary metabolites with broad spectra of bioactivities. At least 193 secondary metabolites from quinoa have been identified in the past 40 years. They mainly include phenolic acids, flavonoids, terpenoids, steroids, and nitrogen-containing compounds. These metabolites exhibit many physiological functions, such as insecticidal, molluscicidal and antimicrobial activities, as well as various kinds of biological activities such as antioxidant, cytotoxic, anti-diabetic and anti-inflammatory properties. This review focuses on our knowledge of the structures, biological activities and functions of quinoa secondary metabolites. Biosynthesis, development and utilization of the secondary metabolites especially from quinoa bran were prospected.
Effect of Different Proportions of Three Microbial Agents on Ammonia Mitigation during the Composting of Layer Manure
Authors: Zhou Shizheng , Zhang Xinyi , Liao Xindi , Wu Yinbao , Mi Jiandui , Wang Yan
Published: July 2019

Odor emissions represent one of the important issues of aerobic composting. The addition of microbial agents to compost is an important method for solving this problem, but this process is often unstable when a single microbial agent is added to the compost. Therefore, in this study, five treatments comprising different proportions of Bacillus stearothermophilus, Candida utilis, and Bacillus subtilis were tested to determine the best combination of the three microbial agents for ammonia reduction, as follows: control group (CK), 2:1:1 (A), 1:1:2 (B), 1:2:1 (C), and 1:1:1 (D). Compared with the CK group, the A, B, C, and D groups reduced ammonia emissions by 17.02, 9.68, 53.11, and 46.23%, respectively. The total ammonia emissions were significantly lower in C and D than in CK (p < 0.05). These two treatment groups had significantly increased nitrate nitrogen concentrations and decreased pH values and ammonium nitrogen concentrations (p < 0.05). Throughout the composting process, the total bacterial number was significantly higher in C and D than in CK (p < 0.05). Therefore, it is likely that B. stearothermophilus, C. utilis, and B. subtilis compounded from 1:2:1 (C) to 1:1:1 (D) reduced the ammonia emissions due to (1) a reduction in the pH and (2) the promotion of the growth of ammonia-oxidizing bacteria and the conversion of ammonium nitrogen to nitrate nitrogen. This study provides a theoretical basis and technical support for the odor problem of layer manure compost and promotes the development of composting technology.
On the Aggregation and Sensing Properties of Zinc(II) Schiff-Base Complexes of Salen-Type Ligands
Authors: Consiglio Giuseppe , Oliveri Ivan Pietro , Failla Salvatore , Di Bella Santo
Published: July 2019

The zinc(II) ion forms stable complexes with a wide variety of ligands, but those related to Schiff-bases are among the most largely investigated. This review deals with the peculiar aggregation characteristics of Zn(II) Schiff-base complexes from tetradentate N2O2 salen-type ligands, L, derivatives from salicylaldehydes and 1,2-diamines, and is mostly focused on their spectroscopic properties in solution. Thanks to their Lewis acidic character, ZnL complexes show interesting structural, nanostructural, and aggregation/deaggregation properties in relation to the absence/presence of a Lewis base. Deaggregation of these complexes is accompanied by relevant changes of their spectroscopic properties that can appropriately be exploited for sensing Lewis bases. Thus, ZnL complexes have been investigated as chromogenic and fluorogenic chemosensors of charged and neutral Lewis bases, including cell imaging, and have shown to be selective and sensitive to the Lewis basicity of the involved species. From these studies emerges that these popular, Lewis acidic bis(salicylaldiminato)Zn(II) Schiff-base complexes represent classical coordination compounds for modern applications.
Inhibitory Activity of Plant Essential Oils against E. coli 1-Deoxy-d-xylulose-5-phosphate reductoisomerase
Authors: Yan Ge , Zhu Bo-Rong , Tian Fang-Lin , Hui Xian , Li Heng , Li Yi-Ming , Gao Wen-Yun
Published: July 2019

The rate-limiting enzyme of the 2-methyl-d-erythritol-4-phosphate (MEP) terpenoid biosynthetic pathway, 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), provides the perfect target for screening new antibacterial substances. In this study, we tested the DXR inhibitory effect of 35 plant essential oils (EOs), which have long been recognized for their antimicrobial properties. The results show that the EOs of Zanbthoxylum bungeanum (ZB), Schizonepetae tenuifoliae (ST), Thymus quinquecostatus (TQ), Origanum vulgare (OV), and Eugenia caryophyllata (EC) displayed weak to medium inhibitory activity against DXR, with IC50 values of 78 μg/mL, 65 μg/mL, 59 μg/mL, 48 μg/mL, and 37 μg/mL, respectively. GC-MS analyses of the above oils and further DXR inhibitory activity tests of their major components revealed that eugenol (EC) and carvacrol (TQ and OV) possess medium inhibition against the protein (68.3% and 55.6%, respectively, at a concentration of 20 μg/mL), whereas thymol (ST, TQ, and OV), carveol (ZB), and linalool (ZB, ST, and OV) only exhibited weak inhibition against DXR, at 20 μg/mL (23%−26%). The results add more details to the antimicrobial mechanisms of plant EOs, which could be very helpful in the direction of the reasonable use of EOs in the food industry and in the control of phytopathogenic microbials.
Titania-Catalyzed H2O2 Thermal Oxidation of Styrenes to Aldehydes
Authors: Ito Satoru , Kon Yoshihiro , Nakashima Takuya , Hong Dachao , Konno Hideo , Ino Daisuke , Sato Kazuhiko
Published: July 2019

We investigated the selective oxidation of styrenes to benzaldehydes by using a non-irradiated TiO2–H2O2 catalytic system. The oxidation promotes multi-step reactions from styrenes, including the cleavage of a C=C double bond and the addition of an oxygen atom selectively and stepwise to provide the corresponding benzaldehydes in good yields (up to 72%). These reaction processes were spectroscopically shown by fluorescent measurements under the presence of competitive scavengers. The absence of the signal from OH radicals indicates the participation of other oxidants such as hydroperoxy radicals (•OOH) and superoxide radicals (•O2−) into the selective oxidation from styrene to benzaldehyde.
Evaluation of Anti-Tumorigenic Effects of Diosmetin against Human Colon Cancer Xenografts in Athymic Nude Mice
Authors: Koosha Sanaz , Mohamed Zahurin , Sinniah Ajantha , Alshawsh Mohammed A.
Published: July 2019

Colon cancer is the third most common type of cancer in the world. Diosmetin (Dis), a natural O-methylated flavone, has been reported to have anti-cancer effects against different types of cancer. Although the mechanisms of action of Dis against several cancer cell lines are well reported, in vivo anti-tumorigenesis properties of this compound are still obscure. Therefore, this study aimed to investigate the anti-tumorigenesis properties of Dis against HCT-116 colon cancer xenografts in nude mice. HCT-116 colon cancer cells were injected in NCr nu/nu nude mice and treatment with Dis was initiated after the tumor volumes reached 100 mm3 and continued for four weeks. On the sacrificing date nude mice treated with 100 mg/kg of Dis showed significant lower tumor volume (264 ± 238.3 mm3) as compared to the untreated group (1428.8 ± 459.6 mm3). Anti-apoptotic Bcl-2 protein was significantly downregulated, while apoptotic protein (Bax) was significantly overexpressed in nude mice treated with 100 mg/kg Dis as compared to untreated mice. In conclusion, our in vivo results indicate that Dis significantly reduces tumor growth rate of HCT-116 colon cancer cells in nude mice at a dose of 100 mg/kg, and has no toxic effects in ICR mice up to 2000 mg/kg.
Cis/Trans Energetics in Epoxide, Thiirane, Aziridine and Phosphirane Containing Cyclopentanols: Effects of Intramolecular OH⋯O, S, N and P Contacts
Authors: Smith Ben E. , Carr Jeremy M. , Tschumper Gregory S.
Published: July 2019

A recent computational analysis of the stabilizing intramolecular OH⋯O contact in 1,2-dialkyl-2,3-epoxycyclopentanol diastereomers has been extended to thiiriane, aziridine and phosphirane analogues. Density functional theory (DFT), second-order Møller-Plesset perturbation theory (MP2) and CCSD(T) coupled-cluster computations with simple methyl and ethyl substituents indicate that electronic energies of the c i s isomers are lowered by roughly 3 to 4 kcal mol−1 when the OH group of these cyclopentanol systems forms an intramolecular contact with the O, S, N or P atom on the adjacent carbon. The results also suggest that S and P can participate in these stabilizing intramolecular interactions as effectively as O and N in constrained molecular environments. The stabilizing intramolecular OH⋯O, OH⋯S, OH⋯N and OH⋯P contacts also increase the covalent OH bond length and significantly decrease the OH stretching vibrational frequency in every system with shifts typically on the order of −41 cm−1.
Endlicheria bracteolata (Meisn.) Essential Oil as a Weapon Against Leishmania amazonensis: In Vitro Assay
Authors: Rottini Mariana Margatto , Amaral Ana Claudia Fernandes , Ferreira José Luiz Pinto , Oliveira Edinilze Souza Coelho , Silva Jefferson Rocha de Andrade , Taniwaki Noemi Nosomi , dos Santos Arith Ramos , Almeida-Souza Fernando , de Souza Celeste da Silva Freitas , Calabrese Kátia da Silva
Published: July 2019

The difficulties encountered and the numerous side effects present in the treatment of cutaneous leishmaniasis have encouraged the research for new compounds that can complement or replace existing treatment. The growing scientific interest in the study of plants, which are already used in folk remedies, has led our group to test Endlicheria bracteolata essential oil against Leishmania amazonensis. Several species of the Lauraceae family, or their compounds, have relevant antiprotozoal activities Therefore, the biological potential on L. amazonensis forms from the essential oil of Endlicheria bracteolata leaves was verified for the first time in that work. The antileishmanial activity was evaluated against promastigotes and intracellular amastigotes, and cytotoxicity were performed with J774.G8, which were incubated with different concentrations of E. bracteolata essential oil. Transmission electron microscopy and flow cytometry were performed with E. bracteolata essential oil IC50. Promastigote forms showed E. bracteolata essential oil IC50 of 7.945 ± 1.285 µg/mL (24 h) and 6.186 ± 1.226 µg/mL (48 h), while for intracellular amastigote forms it was 3.546 ± 1.184 µg/mL (24 h). The CC50 was 15.14 ± 0.090 µg/mL showing that E. bracteolata essential oil is less toxic to macrophages than to parasites. Transmission electron microscopy showed that E. bracteolata essential oil treatment is capable of inducing mitochondrial damage to promastigote and intracellular amastigote forms, while flow cytometry showed ΔѰm disruption in treated parasites. These results could bring about new possibilities to develop products based on E. bracteolata essential oil to treat cutaneous leishmaniasis, especially for people who cannot receive the conventional therapy.
Controlled Preparation of Nanoparticle Gradient Materials by Diffusion
Authors: Spinnrock Andreas , Martens Max , Enders Florian , Boldt Klaus , Cölfen Helmut
Published: July 2019

Nanoparticle gradient materials combine a concentration gradient of nanoparticles with a macroscopic matrix. This way, specific properties of nanoscale matter can be transferred to bulk materials. These materials have great potential for applications in optics, electronics, and sensors. However, it is challenging to monitor the formation of such gradient materials and prepare them in a controlled manner. In this study, we present a novel universal approach for the preparation of this material class using diffusion in an analytical ultracentrifuge. The nanoparticles diffuse into a molten thermoreversible polymer gel and the process is observed in real-time by measuring the particle concentrations along the length of the material to establish a systematic understanding of the gradient generation process. We extract the apparent diffusion coefficients using Fick’s second law of diffusion and simulate the diffusion behavior of the particles. When the desired concentration gradient is achieved the polymer solution is cooled down to fix the concentration gradient in the formed gel phase and obtain a nanoparticle gradient material with the desired property gradient. Gradients of semiconductor nanoparticles with different sizes, fluorescent silica particles, and spherical superparamagnetic iron oxide nanoparticles are presented. This method can be used to produce tailored nanoparticle gradient materials with a broad range of physical properties in a simple and predictable way.
Tunable Duplex Metalens Based on Phase-Change Materials in Communication Range
Authors: Bai Wei , Yang Ping , Wang Shuai , Huang Jie , Chen Dingbo , Zhang Zhaojian , Yang Junbo , Xu Bing
Published: July 2019

Metalenses recently have attracted attention because of their more compact size in comparison with conventional lenses; they can also achieve better optical performance with higher resolution. Duplexer is an interesting function of a metalens that can distinguish different sources and divide them into two parts for specific purposes. In this article, we design tunable duplex metalenses with phase-change material Ge2Sb2Te5 for the first time. Two types of special unit cells are designed to modulate the incident lights, and four metalenses are designed based on the two types of unit cells. Specific phase profiles are calculated for different sections of metalens in which the corresponding unit cells are settled; accordingly, the metalenses can focus the incident lights at any positions according to our design. Moreover, the metalenses become selectable via tuning the state of phase-change material, which means that the output light field can be actively controlled. The proposal of our tunable duplex metalenses will offer new opportunities for active three-dimensional imaging or optical coding.
Estimation of Energy Intake by a Food Frequency Questionnaire: Calibration and Validation with the Doubly Labeled Water Method in Japanese Older People
Authors: Watanabe Daiki , Nanri Hinako , Sagayama Hiroyuki , Yoshida Tsukasa , Itoi Aya , Yamaguchi Miwa , Yokoyama Keiichi , Watanabe Yuya , Goto Chiho , Ebine Naoyuki , Higaki Yasuki , Ishikawa-Takata Kazuko , Kimura Misaka , Yamada Yosuke ,   Kyoto-Kameoka Study Group
Published: July 2019

Accurate assessments of a target population’s energy intake (EI) are essential to prevent poor nutritional status. However, self-reported dietary records (DRs) or food frequency questionnaires (FFQs) are not always accurate, thereby requiring validation and calibration studies. This study aimed to validate the EI estimated by a FFQ using the doubly labeled water (DLW) method. Participants were 109 Japanese older adults (50 women and 59 men) aged 65–88 years. The EI was obtained by a DR and 47-item FFQ over 1 year. The total energy expenditure (TEE) was measured by DLW for ~2 weeks. EI was significantly lower than TEE (p < 0.01); ratios of EI assessed by DR and FFQ against TEE were 0.91 ± 0.17 and 0.82 ± 0.22, respectively. TEE was significantly and moderately correlated with the EI estimated by the DR (r = 0.45, p < 0.01) and FFQ (r = 0.37, p < 0.01). Furthermore, the EI correlation coefficients estimated by DR and the FFQ in this study were not significantly different (p = 0.46). The EI/TEE ratio was significantly and negatively correlated with the body mass index (BMI). In conclusion, EI estimated with a DR or FFQ modestly correlated with TEE, and calibrating EI with a developed equation in this study can attenuate the underestimation of EI.
Gestational Diabetes Mellitus: The Impact of Carbohydrate Quality in Diet
Authors: Filardi Tiziana , Panimolle Francesca , Crescioli Clara , Lenzi Andrea , Morano Susanna
Published: July 2019

Gestational diabetes mellitus (GDM) is defined as “glucose intolerance that is first diagnosed during pregnancy”. Mothers with GDM and their infants may experience both short and long term complications. Dietary intervention is the first therapeutic strategy. If good glycaemic control is not achieved, insulin therapy is recommended. There is no consensus on which nutritional approach should be used in GDM. In the last few years, there has been growing evidence of the benefits of a low glycaemic index (LGI) diet on diabetes and cardiovascular disease. The effect of a LGI diet on GDM incidence has been investigated as well. Several studies observed a lower incidence of GDM in LGI diet arms, without adverse maternal and fetal outcomes. The main positive effect of the LGI diet was the reduction of 2-h post-prandial glucose (PPG). Several studies have also evaluated the effect of the LGI diet in GDM treatment. Overall, the LGI diet might have beneficial effects on certain outcomes, such as 2-h PPG, fasting plasma glucose and lipid profile in patients with GDM. Indeed, most studies observed a significant reduction in insulin requirement. Overall, according to current evidence, the LGI nutritional approach is safe and it might therefore be considered in clinical care for GDM.
Changes in Nutritional Status and Musculoskeletal Health in a Geriatric Post-Fall Care Plan Setting
Authors: Conzade Romy , Phu Steven , Vogrin Sara , Bani Hassan Ebrahim , Sepúlveda-Loyola Walter , Thorand Barbara , Duque Gustavo
Published: July 2019

Understanding how changes in nutritional status influence musculoskeletal recovery after falling remains unclear. We explored associations between changes in nutritional status and musculoskeletal health in 106 community-dwelling older adults aged ≥65 years, who attended the Falls and Fractures Clinic at Sunshine Hospital in St Albans, Australia after falling. At baseline and after 6 months, individuals were assessed for Mini Nutritional Assessment (MNA®), grip strength, gait speed, Timed Up and Go (TUG) test, Short Physical Performance Battery (SPPB), and bone turnover marker levels. Associations were examined using multiple linear regression, adjusted for baseline covariates and post-fall care plans. Over 6 months, the prevalence of malnutrition or risk thereof decreased from 29% to 15% using MNA <24/30. Specifically, 20 individuals (19%) improved, 7 (7%) deteriorated, and 73 (69%) maintained nutritional status, including 65 (61%) who remained well-nourished and 8 (8%) who remained malnourished/at risk. A 1-point increase in MNA score over 6 months was associated with an increase of 0.20 points (95% confidence interval 0.10, 0.31, p < 0.001) in SPPB score. Improvement in nutritional status was associated with improvement in physical performance, providing a basis for interventional studies to ascertain causality and evaluate nutritional models of care for post-fall functional recovery in older adults.
Effect of Sulfonic Groups Concentration on IEC Properties in New Fluorinated Copolyamides
Authors: Pali-Casanova Ramón , Yam-Cervantes Marcial , Zavala-Loría. José , Loría-Bastarrachea María , Aguilar-Vega Manuel , Dzul-López Luis , Sámano-Celorio María , Crespo-Álvarez Jorge , García-Villena Eduardo , Agudo-Toyos Pablo , Méndez-Martínez Francisco
Published: July 2019

Seven aromatic polyamides and copolyamides were synthesized from two different aromatic diamines: 4,4′-(Hexafluoroisopropylidene)bis(p-phenyleneoxy)dianiline (HFDA) and 2,4-Aminobenzenesulfonic acid (DABS). The synthesis was carried out by polycondensation using isophthaloyl dichloride (1SO). The effect of an increasing molar concentration of the sulfonated groups, from DABS, in the copolymer properties was evaluated. Inherent viscosity tests were carried out to estimate molecular weights. Mechanical tests were carried out under tension, maximum strength ( σ max), Young’s modulus (E), and elongation at break (εmax) to determine their mechanical properties. Tests for water sorption and ion exchange capacity (IEC) were carried out. Proton conductivity was measured using electrochemical impedance spectroscopy (EIS). The results indicate that as the degree of sulfonation increase, the greater the proton conductivity. The results obtained showed conductivity values lower than the commercial membrane Nafion 115 of 0.0065 S cm−1. The membrane from copolyamide HFDA/DABS/1S0-70/30 with 30 mol DABS obtained the best IEC, with a value of 0.747 mmol g−1 that resulted in a conductivity of 2.7018 × 10−4 S cm−1, lower than the data reported for the commercial membrane Nafion 115. According to the results obtained, we can suggest that further developments increasing IEC will render membranes based on aromatic polyamides that are suitable for their use in PEM fuel cells.
Validation of Satellite, Reanalysis and RCM Data of Monthly Rainfall in Calabria (Southern Italy)
Authors: Caroletti Giulio Nils , Coscarelli Roberto , Caloiero Tommaso
Published: July 2019

Skills in reproducing monthly rainfall over Calabria (southern Italy) have been validated for the Climate Hazards group InfraRed Precipitation with Station data (CHIRPS) satellite data, the E-OBS dataset and 13 Global Climate Model-Regional Climate Model (GCM-RCM) combinations, belonging to the ENSEMBLES project output set. To this aim, 73 rainfall series for the period 1951–1980 and 79 series for the period 1981–2010 have been selected from the database managed by Multi-Risk Functional Centre of the Regional Agency for Environmental Protection (Regione Calabria). The relative mean and standard deviation errors, and the Pearson correlation coefficient have been used as validation metrics. Results showed that CHIRPS satellite data (available only for the 1981–2010 validation period) and RCMs based on the ECHAM5 Global Climate performed better both in mean error and standard deviation error compared to other datasets. Moreover, a slight appreciable improvement in performance for all ECHAM5-based models and for the E-OBS dataset has been observed in the 1981–2010 time-period. The whole validation-and-assessment procedure applied in this work is general and easily applicable where ground data and gridded data are available. This procedure might help scientists and policy makers to select among available datasets those best suited for further applications, even in regions with complex orography and an inadequate amount of representative stations.
Improving the Transferability of Suspended Solid Estimation in Wetland and Deltaic Waters with an Empirical Hyperspectral Approach
Authors: Jensen Daniel , Simard Marc , Cavanaugh Kyle , Sheng Yongwei , Fichot Cédric , Pavelsky Tamlin , Twilley Robert
Published: July 2019

The deposition of suspended sediment is an important process that helps wetlands accrete surface material and maintain elevation in the face of sea level rise. Optical remote sensing is often employed to map total suspended solids (TSS), though algorithms typically have limited transferability in space and time due to variability in water constituent compositions, mixtures, and inherent optical properties. This study used in situ spectral reflectances and their first derivatives to compare empirical algorithms for estimating TSS using hyperspectral and multispectral data. These algorithms were applied to imagery collected by NASA’s Airborne Visible/Infrared Imaging Spectrometer-Next Generation (AVIRIS-NG) over coastal Louisiana, USA, and validated with a multiyear in situ dataset. The best performing models were then applied to independent spectroscopic data collected in the Peace–Athabasca Delta, Canada, and the San Francisco Bay–Delta Estuary, USA, to assess their robustness and transferability. A derivative-based partial least squares regression (PLSR) model applied to simulated AVIRIS-NG data showed the most accurate TSS retrievals (R2 = 0.83) in these contrasting deltaic environments. These results highlight the potential for a more broadly applicable generalized algorithm employing imaging spectroscopy for estimating suspended solids.
A Building Extraction Approach Based on the Fusion of LiDAR Point Cloud and Elevation Map Texture Features
Authors: Lai Xudong , Yang Jingru , Li Yongxu , Wang Mingwei
Published: July 2019

Building extraction is an important way to obtain information in urban planning, land management, and other fields. As remote sensing has various advantages such as large coverage and real-time capability, it becomes an essential approach for building extraction. Among various remote sensing technologies, the capability of providing 3D features makes the LiDAR point cloud become a crucial means for building extraction. However, the LiDAR point cloud has difficulty distinguishing objects with similar heights, in which case texture features are able to extract different objects in a 2D image. In this paper, a building extraction method based on the fusion of point cloud and texture features is proposed, and the texture features are extracted by using an elevation map that expresses the height of each point. The experimental results show that the proposed method obtains better extraction results than that of other texture feature extraction methods and ENVI software in all experimental areas, and the extraction accuracy is always higher than 87%, which is satisfactory for some practical work.
Optimized Stratification for Mapping Riparian Vegetation in Arid and Semiarid Environments
Authors: McGwire Kenneth C.
Published: July 2019

This paper describes a method of mapping riparian vegetation in semi-arid to arid environments using the Landsat normalized difference vegetation index (NDVI). The method successfully identified a range of riparian community types across the entire state of Nevada, USA, which spans 7 degrees of latitude and almost 4000 m of elevation. The landscape was stratified into units of similar elevation and solar exposure, and riparian areas were identified as having anomalously high NDVI within a local neighborhood. Thousands of calibration points were used in a simplex optimization to select the spatial neighborhood, the elevation and insolation strata, the minimum NDVI to be considered as potentially riparian, the number of standard deviations from the mean for an anomaly to be classified as riparian, and a limit on upslope position. Mapping of subpixel riparian corridors was improved by applying a directional high-pass filter to the NDVI data. Irrigated areas in agricultural and urban areas were removed based on land ownership maps and manual editing. The final map was tested with 400 independent test points: producer’s accuracy was 84.6% and user’s accuracy was 93.5%. This method should be broadly useful for mapping riparian features across large and complex regions with arid to semi-arid environments.
Performance Analysis of Time Synchronization Protocols in Wireless Sensor Networks
Authors: Phan Linh-An , Kim Taejoon , Kim Taehong , Lee JaeSeang , Ham Jae-Hyun
Published: July 2019

The time synchronization protocol is indispensable in various applications of wireless sensor networks, such as scheduling, monitoring, and tracking. Numerous protocols and algorithms have been proposed in recent decades, and many of them provide micro-scale resolutions. However, designing and implementing a time synchronization protocol in a practical wireless network is very challenging compared to implementation in a wired network; this is because its performance can be deteriorated significantly by many factors, including hardware quality, message delay jitter, ambient environment, and network topology. In this study, we measure the performance of the Flooding Time Synchronization Protocol (FTSP) and Gradient Time Synchronization Protocol (GTSP) in terms of practical network conditions, such as message delay jitter, synchronization period, network topology, and packet loss. This study provides insights into the operation and optimization of time synchronization protocols. In addition, the performance evaluation identifies that FTSP is highly affected by message delay jitter due to error accumulation over multi-hops. We demonstrate that the proposed extended version of the FTSP (E-FTSP) alleviates the effect of message delay jitter and enhances the overall performance of FTSP in terms of error, time, and other factors.