bluuebunny/crossref_metadata_2025 huggingface dataset:
Experiencing Event Management During the Coronavirus Pandemic: A Public Sector Perspective
Authors: Coles Tim , Garcia Giselle , O'Malley Evelyn , Turner Cathy
Published: January 2022

Events have played a significant role in the way in which the Coronavirus pandemic has been experienced and known around the world. Little is known though about how the pandemic has impacted on supporting, managing and governing events in municipal (i.e., local) authorities as key stakeholders, nor how events have featured in the opening-up of localities. This paper reports on empirical research with senior events officers for local authorities in the UK on these key knowledge gaps. Specifically, it examines events officers' unfolding experiences of the pandemic. The paper points to unpreparedness for a crisis of this scale and magnitude, and the roles of innovation, adaptation and co-production in the emergent response. It highlights the transformative nature of the pandemic through reconsiderations of the purpose of public sector involvement in events and, from a policy perspective, how relatively smaller-scale, more agile and lower-risk arts events and performances can figure in local recovery. Finally, while the effects on, and response of, the body corporate (the local authority) to crises is an obvious focus, it is important to recognise those of the individuals who manage the response and drive change.
An Enhanced Sliding Mode Speed Control for Induction Motor Drives
Authors: Shiravani Fahimeh , Alkorta Patxi , Cortajarena Jose Antonio , Barambones Oscar
Published: January 2022

In this paper, an enhanced Integral Sliding Mode Control (ISMC) for mechanical speed of an Induction Motor (IM) is presented and experimentally validated. The design of the proposed controller has been done in the d-q synchronous reference frame and indirect Field Oriented Control (FOC). Global asymptotic speed tracking in the presence of model uncertainties and load torque variations has been guaranteed by using an enhanced ISMC surface. Moreover, this controller provides a faster speed convergence rate compared to the conventional ISMC and the Proportional Integral methods, and it eliminates the steady-state error. Furthermore, the chattering phenomenon is reduced by using a switching sigmoid function. The stability of the proposed controller under parameter uncertainties and load disturbances has been provided by using the Lyapunov stability theory. Finally, the performance of this control method is verified through numerical simulations and experimental tests, getting fast dynamics and good robustness for IM drives.
Design and Experimental Analysis of Charge Recovery for Piezoelectric Fan
Authors: Chen Zhenwei , Tang Wei , Li Ze , Lan Jiaqi
Published: January 2022

The piezoelectric (PE) fan is widely adopted in the field of microelectronics cooling due to its advantages of high reliability and good heat dissipation characteristics. However, PE fans driven by conventional circuits suffer from plenty of energy loss. To save energy, we propose an inductor-based charge recovery method and apply it to the driving circuit for the PE fan. Two inductor-based driving circuits, a single inductor-based driving (SID) circuit and a double inductor-based driving (DID) circuit are compared. The SID circuit has a simple structure and a slightly higher energy-saving rate, while the DID circuit introduces no additional oscillations and is more stable. The experimental results show that when the supply voltage changes, both circuits have a relatively stable energy-saving rate, which is about 30% for the SID circuit and 28% for the DID circuit. Moreover, the proposed circuits enjoy the same driving capacity as the conventional circuit, and the driven fan has the same cooling performance.
Range and Accuracy Improvement of Artillery Rocket Using Fixed Canards Trajectory Correction Fuze
Authors: Raza Ali , Wang Hua
Published: January 2022

This paper presents a two-phase guidance and control algorithm to extend the range and improve the impact point accuracy of a 122-mm rocket using a fixed canards trajectory correction fuze. The guidance algorithm consists of a unique glide and correction phase of the rocket trajectory that is activated after the flight’s apex. The glide phase operates in an open-loop configuration where guidance commands are generated to increase the range of the rocket. In contrast, the correction phase operates in a closed-loop configuration where the Impact Point Prediction method based on Modified Projectile Linear Theory is used as a feedback channel to correct the range and drift errors. The proposed fixed canards trajectory correction fuze has a simple and reliable single channel roll-orientation control configuration. The rocket trajectory model consists of a 7-DOF non-linear dynamic model of a dual-spin rocket configuration with a fixed canards correction fuze mounted at the nose. A Monte Carlo simulation of the rocket’s inertial and launch point perturbations show that the fixed canards fuze with the proposed guidance algorithm can double the range of the rocket without changing the rocket motor thrust-time curve. At the same time, the rocket’s accuracy can also be improved beyond the results of an unguided rocket.
Factors Affecting the Change of Agricultural Land Use to Tourism: A Case Study on the Southern Coasts of the Caspian Sea, Iran
Authors: Ghadami Mostafa , Dittmann Andreas , Pazhuhan Mousa , Aligholizadeh Firouzjaie Naser
Published: January 2022

This research investigates the reasons of changing the agricultural land use to tourism in a developing country with different political, economic and social context (Iran). The method used in this research is qualitative, and unstructured interviews have been used to collect data. The target population of the research includes farmers who have sold their farmlands to investors in the tourism sector and experts from the agricultural department of the relevant county. The interviewees have been selected through using snowball method and after reaching theoretical saturation, the data collection process was stopped. The results showed that various macro and micro factors affected the process of changing the agricultural land uses to tourism, including the weakness of the agricultural sector in creating income and job opportunities compared to the tourism sector, the weakness of the land use laws and the lack of inter-organizational coordination in law enforcement, the weakness of the property registration system and the lack of a national cadaster, the lack of effective government support of the agricultural sector, the rapid rise in land prices and, ultimately, the change in the attitude of farmers both old and young once towards the agricultural activity and the level of welfare.
Grass–Legume Mixtures Show Potential to Increase Above- and Belowground Biomass Production for Andean Forage-Based Fallows
Authors: Meza Katherin , Vanek Steven J. , Sueldo Yulissa , Olivera Edgar , Ccanto Raúl , Scurrah Maria , Fonte Steven J.
Published: January 2022

Soils of the Andean highlands are under threat from cropping system intensification. Improved forage-based fallows offer great promise to address this issue, but research is needed to better understand the potential of species mixtures vs. monocultures to support multiple farmer objectives, especially forage production and soil conservation. We used a pot study to quantify above- and belowground biomass production as well as the total N uptake of grass–legume pairs between five grasses: (1) oat (Avena sativa), (2) ryegrass (Lolium multiflorum), (3) festulolium (Lolium × Festuca genera), (4) brome grass (Bromus catharticus), and (5) orchard grass (Dactylis glomerata), and four legumes: (1) vetch (Vicia dasycarpa), (2) red clover (Trifolium pratense), (3) black medic (Medicago lupulina), and (4) alfalfa (Medicago sativa) relative to the performance of each species in monoculture within two soils from the central Peruvian Andes. Grass–legume bicultures demonstrated significant overyielding, producing 65% and 28% more total dry biomass and total N uptake on average than monocultures. Aboveground biomass of bicultures was significantly influenced by the species of legume present, while belowground biomass was more affected by the grass species in the mixture. When evaluating the growth of each species separately, our findings indicate that overyielding was driven more by the enhanced growth of grasses relative to legumes. Our findings indicate that combining key functional groups (e.g., grass and legume, annual and perennial) offers great promise for developing improved fallows for supporting soil health and productivity in Andean agroecosystems.
Anatomical and Biochemical Traits Associated with Field Resistance of Onion Cultivars to Onion Thrips and the Effect of Mechanical Injury on the Level of Biochemical Compounds in Onion Leaves
Authors: Pobożniak Maria , Olczyk Marta , Wójtowicz Tomasz , Kamińska Iwona , Hanus-Fajerska Ewa , Kostecka-Gugała Anna , Kruczek Michał
Published: January 2022

Thrips tabaci Lind. is a global pest and also represents a serious threat to onion production in Poland. In 2 years (2015–2016) of field studies, 8 onion cultivars were evaluated to characterize the resistance to onion thrips and to determine if any biochemical and anatomical features of onion plants are associated with antixenotic and/or antibiotic mechanisms of resistance. Additionally, the influence of mechanical injury on the content of several biochemical compounds in onion leaves was made. The resistance to thrips colonization during the migration period, abundance, and thrips damage throughout the whole vegetation season was determined. We identified two cultivars, Tęcza and Wenta, resistant to thrips colonization and abundance, and one cultivar Wenta resistant to thrips damage. A positive correlation between concentrations of the reducing sugars and thrips abundance and conversely negative relationships between the total phenolic content and thrips damage was confirmed in both years. We suspect that a thinner epidermal layer, a smaller area of epidermal and mesophilic cells, and a lower diameter of vascular bundles may favor the resistance of onion cultivars to thrips. Thrips foraging resulted in a decrease in the content of soluble sugars, sucrose, and plant pigments in the leaves of all onion varieties.
Cymbopogon citratus Essential Oil: Its Application as an Antimicrobial Agent in Food Preservation
Authors: Valková Veronika , Ďúranová Hana , Galovičová Lucia , Borotová Petra , Vukovic Nenad L. , Vukic Milena , Kačániová Miroslava
Published: January 2022

Antimicrobial in vitro and in situ efficacies of Cymbopogon citratus essential oil (lemongrass, LGEO) against 17 spoilage microorganisms (bacteria, yeasts and fungi) were evaluated. Additionally, its chemical composition, and antioxidant and antibiofilm activities were investigated. The LGEO exhibited a strong antioxidant activity (84.0 ± 0.1%), and its main constituents were citral (61.5%), geraniol (6.6%) and 1,8-cineole (6.4%). An in vitro antimicrobial evaluation revealed the lowest inhibition zone (1.00 ± 0.00 mm) in Pseudomonas fluorescens, and the highest inhibition zone (18.00 ± 2.46 mm) in Candida krusei. The values for the minimal inhibitory concentration were determined to be the lowest for Salmonella enteritidis and the highest for C. albicans. Furthermore, the concentration of ≥250 µL/L of LGEO suppressed the growth of Penicillium aurantiogriseum, Penicillium expansum, Penicillium chrysogenum and Penicillium italicum. The changes in the molecular structure of the biofilms produced by Pseudomonas fluorescens and Salmonella enteritidis, after their treatment with LGEO, confirmed its action on both biofilm-forming bacteria. Moreover, an in situ antimicrobial activity evaluation displayed the most effective inhibitory effectiveness of LGEO against Micrococcus luteus, Serratia marcescens (250 µL/L) and Penicillium expansum (125, 250 and 500 µL/L) growing on a carrot. Our results suggest that LGEO, as a promising natural antimicrobial agent, can be applied in the innovative packaging of bakery products and different types of vegetables, which combines commonly used packing materials with the addition of LGEO.
Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity
Authors: Le Van Trang , Kim Me-Sun , Jung Yu-Jin , Kang Kwon-Kyoo , Cho Yong-Gu
Published: January 2022

Nowadays, rice production faces significant challenges due to population pressure, global climate change, and outbreak of various pests and diseases. Breeding techniques used to improve rice traits include mutant breeding, cross breeding, heterogeneity, transformation, molecular markers, genome-wide association study (GWAS), and so on. Since the recently developed CRISPR/Cas9 technology can directly target a specific part of a desired gene to induce mutation, it can be used as a powerful means to expand genetic diversity of crops and develop new varieties. So far, CRISPR/Cas9 technology has been used for improving rice characteristics such as high yield, good quality, abundant nutrition, pest and disease resistance, herbicide resistance, and biotic and abiotic stress resistance. This review highlights the mechanisms and optimization of the CRISPR system and its application to rice crop, including resistance to biotic and abiotic stresses, and improved rice quality and yield.
Mutation of Bacillus velezensis Using Corona Discharge
Authors: Wang Ranran , Bian Wei , Hu Zhuran , Wang Lirong , Yuan Chunhong , Takahashi Katsuyuki , Takaki Koichi
Published: January 2022

Bacillus velezensis is a kind of beneficial bacteria that is widely used in agriculture industry. Bacillus velezensis was irradiated with corona discharge generated by a needle-array high-voltage electrode. The results showed an improvement of activity of Bacillus velezensis by the corona discharge treatment was confirmed at an optimum input energy. Mutation of the Bacillus velezensis by the corona discharge treatment was also confirmed through an rRNA sequence alignment analysis. The enzyme activity of the mutated bacteria was greatly improved, which was a positive effect that can meet the production demand.
Salicylic-Acid-Regulated Antioxidant Capacity Contributes to Growth Improvement of Okra (Abelmoschus esculentus cv. Red Balady)
Authors: Youssef Sabry M. , López-Orenes Antonio , Ferrer María A. , Calderón Antonio A.
Published: January 2022

The improvement of crop yield and quality are currently of prime concern to achieve global food security and sustainability. Plant elicitation with salicylic acid (SA) has been shown to play a key role in regulating plant growth and productivity. Here, the effects of foliar SA application (1, 2, and 4 mM) on antioxidant capacity and phenolic metabolism, among other biochemical parameters, were studied in okra leaves. The results showed that SA treatments markedly increase the levels of endogenous free SA and trigger the accumulation of chlorophylls, soluble proteins, proline, antioxidant capacity, and phenolic compounds, alongside the stimulation of the enzymatic activities of phenylalanine ammonia-lyase and peroxidase, with these changes being paralleled by decreased levels of lipid peroxidation. The beneficial effects of SA treatments were also tested on field-grown okra plants. Results revealed that yield-associated agronomic attributes significantly increased upon SA treatments, especially at 2 mM SA. Altogether, these results point to a connection between SA-induced antioxidant system strengthening and crop growth and productivity. Therefore, foliar application of SA seems to be a potent and workable approach to enhance okra crop quality and yield.
New Opportunities in Glycan Engineering for Therapeutic Proteins
Authors: Zhong Xiaotian , D’Antona Aaron M. , Scarcelli John J. , Rouse Jason C.
Published: January 2022

Glycans as sugar polymers are important metabolic, structural, and physiological regulators for cellular and biological functions. They are often classified as critical quality attributes to antibodies and recombinant fusion proteins, given their impacts on the efficacy and safety of biologics drugs. Recent reports on the conjugates of N-acetyl-galactosamine and mannose-6-phosphate for lysosomal degradation, Fab glycans for antibody diversification, as well as sialylation therapeutic modulations and O-linked applications, have been fueling the continued interest in glycoengineering. The current advancements of the human glycome and the development of a comprehensive network in glycosylation pathways have presented new opportunities in designing next-generation therapeutic proteins.
Two New Compounds Containing Pyridinone or Triazine Heterocycles Have Antifungal Properties against Candida albicans
Authors: Mena Laura , Billamboz Muriel , Charlet Rogatien , Desprès Bérangère , Sendid Boualem , Ghinet Alina , Jawhara Samir
Published: January 2022

Candidiasis, caused by the opportunistic yeast Candida albicans, is the most common fungal infection today. Resistance of C. albicans to current antifungal drugs has emerged over the past decade leading to the need for novel antifungal agents. Our aim was to select new antifungal compounds by library-screening methods and to assess their antifungal effects against C. albicans. After screening 90 potential antifungal compounds from JUNIA, a chemical library, two compounds, 1-(4-chlorophenyl)-4-((4-chlorophenyl)amino)-3,6-dimethylpyridin-2(1H)-one (PYR) and (Z)-N-(2-(4,6-dimethoxy-1,3,5-triazin-2-yl)vinyl)-4-methoxyaniline (TRI), were identified as having potential antifungal activity. Treatment with PYR and TRI resulted in a significant reduction of C. albicans bioluminescence as well as the number of fungal colonies, indicating rapid fungicidal activity. These two compounds were also effective against clinically isolated fluconazole- or caspofungin-resistant C. albicans strains. PYR and TRI had an inhibitory effect on Candida biofilm formation and reduced the thickness of the mannan cell wall. In a Caenorhabditis elegans infection model, PYR and TRI decreased the mortality of nematodes infected with C. albicans and enhanced the expression of antimicrobial genes that promote C. albicans elimination. Overall, PYR and TRI showed antifungal properties against C. albicans by exerting fungicidal activities and enhancing the antimicrobial gene expression of Caenorhabditis elegans.
Antimicrobial Peptides Epinecidin-1 and Beta-Defesin-3 Are Effective against a Broad Spectrum of Antibiotic-Resistant Bacterial Isolates and Increase Survival Rate in Experimental Sepsis
Authors: Bolatchiev Albert
Published: January 2022

The antimicrobial peptides human Beta-defensin-3 (hBD-3) and Epinecidin-1 (Epi-1; by Epinephelus coioides) could be a promising tool to develop novel antibacterials to combat antibiotic resistance. The antibacterial activity of Epi-1 + vancomycin against methicillin-resistant Staphylococcus aureus (22 isolates) and Epi-1 + hBD-3 against carbapenem-resistant isolates of Klebsiella pneumoniae (n = 23), Klebsiella aerogenes (n = 17), Acinetobacter baumannii (n = 9), and Pseudomonas aeruginosa (n = 13) was studied in vitro. To evaluate the in vivo efficacy of hBD-3 and Epi-1, ICR (CD-1) mice were injected intraperitoneally with a lethal dose of K. pneumoniae or P. aeruginosa. The animals received a single injection of either sterile saline, hBD-3 monotherapy, meropenem monotherapy, hBD-3 + meropenem, or hBD-3 + Epi-1. Studied peptides showed antibacterial activity in vitro against all studied clinical isolates in a concentration of 2 to 32 mg/L. In both experimental models of murine sepsis, an increase in survival rate was seen with hBD-3 monotherapy, hBD-3 + meropenem, and hBD-3 + Epi-1. For K. pneumoniae-sepsis, hBD-3 was shown to be a promising option in overcoming the resistance of Klebsiella spp. to carbapenems, though more research is needed. In the P. aeruginosa-sepsis model, the addition of Epi-1 to hBD-3 was found to have a slightly reduced mortality rate compared to hBD-3 monotherapy.
Empiric “Three-in-One” Bismuth Quadruple Therapy for Second-Line Helicobacter pylori Eradication: An Intervention Study in Southern Italy
Authors: Losurdo Giuseppe , Lacavalla Ilaria , Russo Francesco , Riezzo Giuseppe , Brescia Irene , Rendina Maria , Ierardi Enzo , Di Leo Alfredo
Published: January 2022

The eradication of Helicobacter pylori (H. pylori) may be difficult due to antibiotic resistance. Indeed, after one failure, a second-line therapy is needed and a bismuth containing quadruple therapy (BQT) with a three-in-one capsule formulation is becoming very popular. Therefore, we aimed to evaluate effectiveness and safety of BQT as a second-line therapy. We recruited consecutive patients with one therapy failure. For ten days patients received the three-in-one BQT Pylera® therapy, in combination with a proton-pump inhibitor (PPI), decided at the choice of the investigator, at full dose bid. The eradication rate was calculated by intention-to-treat (ITT) and per-protocol (PP)analyses and 95% confidence intervals (CI) were calculated. Seventy-three patients were recruited, 41 females and 32 males (mean age 53.0±13.1 years). Fifty-five patients failed triple therapy with amoxicillin and clarithromycin and the remaining 18 received sequential therapy. Seventy-two patients consumed at least 90% of the capsules, while only one did not complete the therapy due to adverse events (nausea and diarrhea). By ITT analysis, BQT was successful in 62 subjects (eradication rate 84.9%, 95%CI 76.7–93.1%). By PP analysis, the eradication rate was 86.1% (95%CI 78.1–94.1%).Adverse events were observed in 14 subjects (20.5%).In conclusion, our report confirmed that BQT is effective as an empiric second-line regimen.
Comparative Molecular Mechanisms of Biosynthesis of Naringenin and Related Chalcones in Actinobacteria and Plants: Relevance for the Obtention of Potent Bioactive Metabolites
Authors: Martín Juan F. , Liras Paloma
Published: January 2022

Naringenin and its glycosylated derivative naringin are flavonoids that are synthesized by the phenylpropanoid pathway in plants. We found that naringenin is also formed by the actinobacterium Streptomyces clavuligerus, a well-known microorganism used to industrially produce clavulanic acid. The production of naringenin in S. clavuligerus involves a chalcone synthase that uses p-coumaric as a starter unit and a P450 monoxygenase, encoded by two adjacent genes (ncs-ncyP). The p-coumaric acid starter unit is formed by a tyrosine ammonia lyase encoded by an unlinked, tal, gene. Deletion and complementation studies demonstrate that these three genes are required for biosynthesis of naringenin in S. clavuligerus. Other actinobacteria chalcone synthases use caffeic acid, ferulic acid, sinapic acid or benzoic acid as starter units in the formation of different antibiotics and antitumor agents. The biosynthesis of naringenin is restricted to a few Streptomycess species and the encoding gene cluster is present also in some Saccharotrix and Kitasatospora species. Phylogenetic comparison of S. clavuligerus naringenin chalcone synthase with homologous proteins of other actinobacteria reveal that this protein is closely related to chalcone synthases that use malonyl-CoA as a starter unit for the formation of red-brown pigment. The function of the core enzymes in the pathway, such as the chalcone synthase and the tyrosine ammonia lyase, is conserved in plants and actinobacteria. However, S. clavuligerus use a P450 monooxygenase proposed to complete the cyclization step of the naringenin chalcone, whereas this reaction in plants is performed by a chalcone isomerase. Comparison of the plant and S. clavuligerus chalcone synthases indicates that they have not been transmitted between these organisms by a recent horizontal gene transfer phenomenon. We provide a comprehensive view of the molecular genetics and biochemistry of chalcone synthases and their impact on the development of antibacterial and antitumor compounds. These advances allow new bioactive compounds to be obtained using combinatorial strategies. In addition, processes of heterologous expression and bioconversion for the production of naringenin and naringenin-derived compounds in yeasts are described.
NXP032 Ameliorates Aging-Induced Oxidative Stress and Cognitive Impairment in Mice through Activation of Nrf2 Signaling
Authors: Lee Jae-Min , Lee Joo Hee , Song Min Kyung , Kim Youn-Jung
Published: January 2022

Aging is a neurodegenerative disease that leads to cognitive impairment, and an increase in oxidative stress as a major cause is an important factor. It has been reported that aging-related cognitive impairment is associated with increased oxidative damage in several brain regions during aging. As a powerful antioxidant, vitamin C plays an important role in preventing oxidative stress, but due to its unstable chemical properties, it is easily oxidized and thus the activity of antioxidants is reduced. In order to overcome this easily oxidized vulnerability, we developed NXP032 (vitamin C/DNA aptamer complex) that can enhance the antioxidant efficacy of vitamin C using an aptamer. We developed NXP032 (vitamin C/DNA Aptamin C320 complex) that can enhance the antioxidant efficacy of vitamin C using an aptamer. In the present study, we evaluated the neuroprotective effects of NXP032 on aging-induced cognitive decline, oxidative stress, and neuronal damage in 17-month-old female mice. NXP032 was orally administered at 200 mg/kg of ascorbic acid and 4 mg/kg of DNA aptamer daily for eight weeks. Before the sacrifice, a cognitive behavioral test was performed. Administration of NXP032 alleviated cognitive impairment, neuronal damage, microglia activity, and oxidative stress due to aging. We found that although aging decreases the Nrf2-ARE pathway, NXP032 administration activates the Nrf2-ARE pathway to increase the expression of SOD-1 and GSTO1/2. The results suggest that the new aptamer complex NXP032 may be a therapeutic intervention to alleviate aging-induced cognitive impairment and oxidative stress.
How Does LC/MS Compare to UV in Coffee Authentication and Determination of Antioxidant Effects? Brazilian and Middle Eastern Coffee as Case Studies
Authors: El-Hawary Enas A. , Zayed Ahmed , Laub Annegret , Modolo Luzia V. , Wessjohann Ludger , Farag Mohamed A.
Published: January 2022

Coffee is a popular beverage owing to its unique flavor and diverse health benefits. The current study aimed at investigating the antioxidant activity, in relation to the phytochemical composition, of authenticated Brazilian green and roasted Coffea arabica and C. robusta, along with 15 commercial specimens collected from the Middle East. Ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI–HRMS) and UV spectrometry were employed for profiling and fingerprinting, respectively. With the aid of global natural product social molecular networking (GNPS), a total of 88 peaks were annotated as belonging to different chemical classes, of which 11 metabolites are reported for the first time in coffee seeds. Moreover, chemometric tools showed comparable results between both platforms, with more advantages for UV in the annotation of roasting products, suggesting that UV can serve as a discriminative tool. Additionally, antioxidant assays coupled with the UHPLC-ESI–HRMS dataset using partial least-squares discriminant analysis (PLS-DA) demonstrated that caffeoylquinic acid and caffeine were potential antioxidant markers in unroasted coffee versus dicaffeoyl quinolactone and melanoidins in roasted coffee. The study presents a multiplex metabolomics approach to the quality control of coffee, one of the most consumed beverages.
Revisiting the Oxidation of Flavonoids: Loss, Conservation or Enhancement of Their Antioxidant Properties
Authors: Speisky Hernan , Shahidi Fereidoon , Costa de Camargo Adriano , Fuentes Jocelyn
Published: January 2022

Flavonoids display a broad range of health-promoting bioactivities. Among these, their capacity to act as antioxidants has remained most prominent. The canonical reactive oxygen species (ROS)-scavenging mode of the antioxidant action of flavonoids relies on the high susceptibility of their phenolic moieties to undergo oxidation. As a consequence, upon reaction with ROS, the antioxidant capacity of flavonoids is severely compromised. Other phenol-compromising reactions, such as those involved in the biotransformation of flavonoids, can also markedly affect their antioxidant properties. In recent years, however, increasing evidence has indicated that, at least for some flavonoids, the oxidation of such residues can in fact markedly enhance their original antioxidant properties. In such apparent paradoxical cases, the antioxidant activity arises from the pro-oxidant and/or electrophilic character of some of their oxidation-derived metabolites and is exerted by activating the Nrf2–Keap1 pathway, which upregulates the cell’s endogenous antioxidant capacity, and/or, by preventing the activation of the pro-oxidant and pro-inflammatory NF-κB pathway. This review focuses on the effects that the oxidative and/or non-oxidative modification of the phenolic groups of flavonoids may have on the ability of the resulting metabolites to promote direct and/or indirect antioxidant actions. Considering the case of a metabolite resulting from the oxidation of quercetin, we offer a comprehensive description of the evidence that increasingly supports the concept that, in the case of certain flavonoids, the oxidation of phenolics emerges as a mechanism that markedly amplifies their original antioxidant properties. An overlooked topic of great phytomedicine potential is thus unraveled.
Easy-to-Use HPLC Method to Measure Intracellular Ascorbic Acid Levels in Human Peripheral Blood Mononuclear Cells and in Plasma
Authors: van Gorkom Gwendolyn , Gijsbers Birgit , Ververs Erik-Jan , El Molla Ahmed , Sarodnik Cindy , Riess Celine , Wodzig Will , Bos Gerard , Van Elssen Catharina
Published: January 2022

Given the growing interest in ascorbic acid (AA), there is a need for a reliable and reproducible method to measure AA status in the human body. Serum AA concentrations do not correlate well with tissue levels, but AA levels in leukocytes do. However, a standard method for clinical application is lacking. This present study describes a method to measure AA in the peripheral blood mononuclear cells (PBMCs) with hydrophilic interaction liquid chromatography (HILIC). The method can also be used in plasma and other leukocyte subsets. The measurements of AA in PBMCs and plasma were performed with HPLC with HILIC separation and UV detection. The sample preparation involved the isolation of PBMCs and lysis and precipitation with acetonitrile. European Medicine Agency guidelines for bioanalytic method validation were followed for the evaluation. A highly precise execution of the method was found with intra- and inter-assay variations at a maximum of 7.8%. In 40 healthy donors, a mean intracellular AA concentration of 7.9 microgram/108 cells was found in PBMCs. A correlation between plasma and PBMC AA concentration was not present (r = 0.22). In conclusion, we developed a convenient, reliable, and reproducible method for the quantitative determination of AA within PBMCs and plasma from human blood.
The Influence of Hyaluronic Acid Adjunctive Therapy of Periodontitis on Salivary Markers of Oxidative Stress: Randomized, Controlled Clinical Trial
Authors: Olszewska-Czyz Iwona , Kralik Kristina , Tota Marin , Prpic Jelena
Published: January 2022

Periodontitis is a common oral disease affecting the tooth-supporting tissues. Bacteria have been long viewed as the main causative factor in its development; however, many investigations have proved that aberrant immune and inflammatory response and the resulting misbalance between the damage caused by reactive oxygen species and the antioxidant capacity of tissues may be an underlying factor in disease progression that reduces healing potential. The objective of the current trial is to assess the outcomes of the addition of hyaluronic acid (HA) to standard non-surgical periodontal therapy (NST) on some major oxidative stress markers in saliva. HA-based gel designed for dental application was used and the measurements were taken after 3 months. HA adjunctive therapy had a significantly greater increase in markers with antioxidant properties as well as total antioxidant capacity compared to standard NST alone. Furthermore, clinically measured levels of gingival inflammation (bleeding on probing-BOP) and periodontal destruction (clinical attachment loss-CAL) were significantly correlated with these markers, and the correlation was negative. This investigation demonstrates that HA may indeed express antioxidant properties and improve the antioxidant capacity of periodontal tissues, thus improving the prognosis for the teeth and the results of periodontal therapy. Further investigations will be necessary to determine the duration of these effects over time.
Potential and Possible Therapeutic Effects of Melatonin on SARS-CoV-2 Infection
Authors: Shchetinin Evgeny , Baturin Vladimir , Arushanyan Eduard , Bolatchiev Albert , Bobryshev Dmitriy
Published: January 2022

The absence of effective drugs for COVID-19 prevention and treatment requires the search for new candidates among approved medicines. Fundamental studies and clinical observations allow us to approach an understanding of the mechanisms of damage and protection from exposure to SARS-CoV-2, to identify possible points of application for pharmacological interventions. In this review we presented studies on the anti-inflammatory, antioxidant, and immunotropic properties of melatonin. We have attempted to present scientifically proven mechanisms of action for the potential therapeutic use of melatonin during SARS-CoV-2 infection. A wide range of pharmacological properties allows its inclusion as an effective addition to the methods of prevention and treatment of COVID-19.
Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
Authors: Novosadova Ekaterina , Dolotov Oleg , Inozemtseva Ludmila , Novosadova Ludmila , Antonov Stanislav , Shimchenko Darya , Bezuglov Vladimir , Vetchinova Anna , Tarantul Vyacheslav , Grivennikov Igor , Illarioshkin Sergey
Published: January 2022

Oxidative stress (OS) is implicated in the pathogenesis of several neurodegenerative diseases. We have previously shown that N-acyl dopamines (N-ADA and N-DDA) protect the neural cells of healthy donors and patients with Parkinson’s disease from OS. In this study, we assessed the effects of N-acyl dopamines on the expression of neurotrophic factors in human-induced pluripotent stem cell-derived neuronal cultures enriched with dopaminergic neurons under conditions of OS induced by hydrogen peroxide. We showed that hydrogen peroxide treatment increased BDNF but not GDNF mRNA levels, while it did not affect the secretion of corresponding proteins into the culture medium of these cells. Application of N-acyl dopamines promoted BDNF release into the culture medium. Under conditions of OS, N-DDA also increased TRKB, TRKC and RET mRNA levels. Furthermore, N-acyl dopamines prevented cell death 24 h after OS induction and promoted the expression of antioxidant enzymes GPX1, GPX7, SOD1, SOD2 and CAT, as well as reduced the BAX/BCL2 mRNA ratio. These findings indicate that stimulation of the expression of neurotrophic factors and their receptors may underlie the neuroprotective effects of N-acyl dopamines in human neurons.
An Efficient Damage Quantification Method for Cylindrical Structures Enhanced by a Dry-Point-Contact Torsional-Wave Transducer
Authors: Shan Shengbo , Pan Yongdong , Xiao Shengyu
Published: January 2022

Quantification of damage sizes in cylindrical structures such as pipes and rods is of paramount importance in various industries. This work proposes an efficient damage quantification method by using a dry-point-contact (DPC) transducer based on the non-dispersive torsional waves in the low-frequency range. Theoretical analyses are first carried out to investigate the torsional wave interaction with different sizes of defects in cylindrical structures. A damage quantification algorithm is designed based on the wave reflections from the defect and end. Capitalizing on multiple excitations at different frequencies, the proposed algorithm constructs a damage image that identifies the geometric parameters of the defects. Numerical simulations are conducted to validate the characteristics of the theoretically-predicted wave-damage interaction analyses as well as the feasibility of the designed damage quantification method. Using the DPC transducer, experiments are efficiently carried out with a simple physical system. The captured responses are first assessed to confirm the capability of the DPC transducer for generating and sensing torsional waves. The sizes of the defects in two representative steel rods are then quantified with the proposed method. Both numerical and experimental results demonstrate the efficacy of the proposed damage quantification method. The understandings of the wave-damage interaction and the concept of the damage quantification algorithm lay out the foundation for engineering applications.
Airspace Geofencing and Flight Planning for Low-Altitude, Urban, Small Unmanned Aircraft Systems
Authors: Kim Joseph , Atkins Ella
Published: January 2022

Airspace geofencing is a key capability for low-altitude Unmanned Aircraft System (UAS) Traffic Management (UTM). Geofenced airspace volumes can be allocated to safely contain compatible UAS flight operations within a fly-zone (keep-in geofence) and ensure the avoidance of no-fly zones (keep-out geofences). This paper presents the application of three-dimensional flight volumization algorithms to support airspace geofence management for UTM. Layered polygon geofence volumes enclose user-input waypoint-based 3-D flight trajectories, and a family of flight trajectory solutions designed to avoid keep-out geofence volumes is proposed using computational geometry. Geofencing and path planning solutions are analyzed in an accurately mapped urban environment. Urban map data processing algorithms are presented. Monte Carlo simulations statistically validate our algorithms, and runtime statistics are tabulated. Benchmark evaluation results in a Manhattan, New York City low-altitude environment compare our geofenced dynamic path planning solutions against a fixed airway corridor design. A case study with UAS route deconfliction is presented, illustrating how the proposed geofencing pipeline supports multi-vehicle deconfliction. This paper contributes to the nascent theory and the practice of dynamic airspace geofencing in support of UTM.
Lower Extremity Kinetics and Kinematics in Runners with Patellofemoral Pain: A Retrospective Case–Control Study Using Musculoskeletal Simulation
Authors: Sinclair Jonathan , Chockalingam Nachiappan , Taylor Paul John
Published: January 2022

Patellofemoral pain (PFP) is a common atraumatic knee pathology in runners, with a complex multifactorial aetiology influenced by sex differences. This retrospective case–control study therefore aimed to evaluate lower limb kinetics and kinematics in symptomatic and control male and female runners using musculoskeletal simulation. Lower extremity biomechanics were assessed in 40 runners with PFP (15 females and 25 males) and 40 controls (15 females and 25 males), whilst running at a self-selected velocity. Lower extremity biomechanics were explored using a musculoskeletal simulation approach. Four intergroup comparisons—(1) overall PFP vs. control; (2) male PFP vs. male control; (3) female PFP vs. female control; and (4) male PFP vs. female PFP—were undertaken using linear mixed models. The overall (stress per mile: PFP = 1047.49 and control = 812.93) and female (peak stress: PFP = 13.07 KPa/BW and control = 10.82 KPa/BW) comparisons showed increased patellofemoral joint stress indices in PFP runners. A significantly lower strike index was also shown in PFP runners in the overall (PFP = 17.75% and control = 33.57%) and female analyses (PFP = 15.49% and control = 40.20%), revealing a midfoot strike in control, and a rearfoot pattern in PFP runners. Peak rearfoot eversion and contralateral pelvic drop range of motion (ROM) were shown to be greater in PFP runners in the overall (eversion: PFP = −8.15° and control = −15.09°/pelvic drop ROM: PFP = 3.64° and control = 1.88°), male (eversion: PFP = −8.05° and control = −14.69°/pelvic drop ROM: PFP = 3.16° and control = 1.77°) and female (eversion: PFP = 8.28° and control = −15.75°/pelvic drop ROM: PFP = 3.64° and control = 1.88°) PFP runners, whilst female PFP runners (11.30°) exhibited a significantly larger peak hip adduction compared to PFP males (7.62°). The findings from this investigation highlight biomechanical differences between control and PFP runners, as well as demonstrating distinctions in PFP presentation for many parameters between sexes, highlighting potential risk factors for PFP that may be addressed through focused intervention modalities, and also the need, where appropriate, for sex-specific targeted treatment approaches.
Design of a Damping Controller Using a Metaheuristic Algorithm for Angle Stability Improvement of an MIB System
Authors: Khawaja Abdul Waheed , Kamari Nor Azwan Mohamed , Zainuri Muhammad Ammirrul Atiqi Mohd
Published: January 2022

Low frequency oscillations in large power systems may result in system instability under large disturbances. Power system stabilisers (PSS) play an effective role in damping these low frequency oscillations by injecting a modulating signal in the excitation loop of a synchronous machine. A new metaheuristic optimisation algorithm termed the sine cosine algorithm (SCA) was proposed for optimising PSS controller parameters to obtain an optimal solution with the damping ratio as an objective function. The SCA technique was examined on a single machine infinite bus (SMIB) system under distinct loading situations and matched with a moth flame optimisation technique and evolutionary programming to design a robust controller of PSS. The simulation was accomplished using a linearised mathematical model of the SMIB. The performance of a designed lead lag-controller of PSS was demonstrated using eigenvalue analysis with simulations, showing promising results. The dynamic performance was validated with respect to the damping ratio, the eigenvalue’s location in the s-plane and rotor angle deviation response to demonstrate system stability.
Spatial Connections between Microplastics and Heavy Metal Pollution within Floodplain Soils
Authors: Weber Collin J. , Hahn Jens , Opp Christian
Published: January 2022

Soils contain an increasing number of different pollutants, which are often released into the environment by human activity. Among the “new” potential pollutants are plastics and microplastics. “Recognized” pollutants such as heavy metals, of geogenic and anthropogenic origin, now meet purely anthropogenic contaminants such as plastic particles. Those can meet especially in floodplain landscapes and floodplain soils, because of their function as a temporary sink for sediments, nutrients, and pollutants. Based on a geospatial sampling approach, we analyzed the soil properties and heavy metal contents (ICP-MS) in soil material and macroplastic particles, and calculated total plastic concentrations (Ptot) from preliminary studies. Those data were used to investigate spatial connections between both groups of pollutants. Our results from the example of the Lahn river catchment show a low-to-moderate contamination of the floodplain soils with heavy metals and a wide distribution of plastic contents up to a depth of two meters. Furthermore, we were able to document heavy metal contents in macroplastic particles. Spatial and statistical correlations between both pollutants were found. Those correlations are mainly expressed by a comparable variability in concentrations across the catchment and in a common accumulation in topsoil and upper soil or sediment layers (0–50 cm). The results indicate comparable deposition conditions of both pollutants in the floodplain system.
Efficiency Enhancement of GaAs Single-Junction Solar Cell by Nanotextured Window Layer
Authors: Kim Chae-Won , Park Gwang-Yeol , Shin Jae-Cheol , Kim Hyo-Jin
Published: January 2022

In order to improve efficiency of flexible III-V semiconductor multi-junction solar cells, it is important to enhance the current density for efficiency improvement and to attain an even efficiency of solar cells on a curved surface. In this study, the nanotextured InAlP window layer of a GaAs single-junction solar cell was employed to suppress reflectance in broad range. The nanotextured surface affects the reflectance suppression with the broad spectrum of wavelength, which causes it to increase the current density and efficiency of the GaAs single-junction solar cell and alleviate the efficiency drop at the high incident angle of the light source. Those results show the potential of the effectively suppressed reflectance of multi-junction solar cells and even performance of solar cells attached on a curved surface.
Laser Fabrication of Titanium Alloy-Based Photothermal Responsive Slippery Surface
Authors: Yi Jian , Zhou Hao , Han Xingchen , Mao Jiangwei , Zhang Yonglai
Published: January 2022

In recent years, biomimetic materials inspired from natural organisms have attracted great attention due to their promising functionalities and cutting-edge applications, emerging as an important research topic. For example, how to reduce the reflectivity of the solid surface and increase the absorption of the substrate surface is essential for developing light response smart surface. Suitable solutions to this issue can be found in natural creatures; however, it is technologically challenging. In this work, inspired from butterfly wings, we proposed a laser processing technology to prepare micro nanostructured titanium alloy surfaces with anti-reflection properties. The reflectivity is significantly suppressed, and thus, the light absorption is improved. Consequently, the anti-reflection titanium alloy surface can be further employed as a photothermal substrate for developing light-responsive slippery surface. The sliding behavior of liquid droplets on the smart slippery surface can be well controlled via light irradiation. This method facilitates the preparation of low-reflection and high-absorption metallic surfaces towards bionic applications.
Intelligent Anomaly Identification of Uplift Pressure Monitoring Data and Structural Diagnosis of Concrete Dam
Authors: Ma Chunhui , Zhao Tianhao , Li Gaochao , Zhang Anan , Cheng Lin
Published: January 2022

As an essential load of the concrete dam, the abnormal change of uplift pressure directly threatens the safety and stability of the concrete dam. Therefore, it is of great significance to accurately and efficiently excavate the hidden information of the uplift pressure monitoring data to clarify the safety state of the concrete dam. Therefore, in this paper, density-based spatial clustering of applications with noise (DBSCAN) method is used to intelligently identify the abnormal occurrence point and abnormal stable stage in the monitoring data. Then, an application method of measured uplift pressure is put forward to accurately reflect the spatial distribution and abnormal position of uplift pressure in the dam foundation. It is easy to calculate and connect with the finite element method through self-written software. Finally, the measured uplift pressure is applied to the finite element model of the concrete dam. By comparing the structural behavior of the concrete dam under the design and measured uplift pressure, the influence of abnormal uplift pressure on the safety state of the concrete dam is clarified, which can guide the project operation. Taking a 98.5 m concrete arch dam in western China as an example, the above analysis ideas and calculation methods have been verified. The abnormal identification method and uplift pressure applying method can provide ideas and tools for the structural diagnosis of a concrete dam.