bluuebunny/crossref_metadata_2025 huggingface dataset:
Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation in Camellia impressinervis
Authors: Zhang Peilan , Chen Shuying , Chen Siyu , Zhu Yuanming , Lin Yuqing , Xu Xinyu , Liu Zhongjian , Zou Shuangquan
Published: March 2024

Real-time quantitative PCR (qRT-PCR) is a pivotal technique for gene expression analysis. To ensure reliable and accurate results, the internal reference genes must exhibit stable expression across varied experimental conditions. Currently, no internal reference genes for Camellia impressinervis have been established. This study aimed to identify stable internal reference genes from eight candidates derived from different developmental stages of C. impressinervis flowers. We employed geNorm, NormFinder, and BestKeeper to evaluate the expression stability of these candidates, which was followed by a comprehensive stability analysis. The results indicated that CiTUB, a tubulin gene, exhibited the most stable expression among the eight reference gene candidates in the petals. Subsequently, CiTUB was utilized as an internal reference for the qRT-PCR analysis of six genes implicated in the petal pigment synthesis pathway of C. impressinervis. The qRT-PCR results were corroborated by transcriptome sequencing data, affirming the stability and suitability of CiTUB as a reference gene. This study marks the first identification of stable internal reference genes within the entire genome of C. impressinervis, establishing a foundation for future gene expression and functional studies. Identifying such stable reference genes is crucial for advancing molecular research on C. impressinervis.
Single Nucleotide Polymorphism in Cell Adhesion Molecule L1 Affects Learning and Memory in a Mouse Model of Traumatic Brain Injury
Authors: Jiang Haoyu , Giarratana Anna O. , Theis Thomas , Nagaraj Vini , Zhou Xiaofeng , Thakker-Varia Smita , Schachner Melitta , Alder Janet
Published: March 2024

The L1 cell adhesion molecule (L1) has demonstrated a range of beneficial effects in animal models of spinal cord injury, neurodegenerative disease, and ischemia; however, the role of L1 in TBI has not been fully examined. Mutations in the L1 gene affecting the extracellular domain of this type 1 transmembrane glycoprotein have been identified in patients with L1 syndrome. These patients suffer from hydrocephalus, MASA (mental retardation, adducted thumbs, shuffling gait, aphasia) symptoms, and corpus callosum agenesis. Clinicians have observed that recovery post-traumatic brain injury (TBI) varies among the population. This variability may be explained by the genetic differences present in the general population. In this study, we utilized a novel mouse model of L1 syndrome with a mutation at aspartic acid position 201 in the extracellular domain of L1 (L1-201). We assessed the impact of this specific single nucleotide polymorphism (SNP) localized to the X-chromosome L1 gene on recovery outcomes following TBI by comparing the L1-201 mouse mutants with their wild-type littermates. We demonstrate that male L1-201 mice exhibit significantly worse learning and memory outcomes in the Morris water maze after lateral fluid percussion (LFP) injury compared to male wild-type mice and a trend to worse motor function on the rotarod. However, no significant changes were observed in markers for inflammatory responses or apoptosis after TBI.
A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST
Authors: Baleeva Nadezhda S. , Bogdanova Yulia A. , Goncharuk Marina V. , Sokolov Anatolii I. , Myasnyanko Ivan N. , Kublitski Vadim S. , Smirnov Alexander Yu. , Gilvanov Aidar R. , Goncharuk Sergey A. , Mineev Konstantin S. , Baranov Mikhail S.
Published: March 2024

NanoFAST is the smallest fluorogen-activating protein, consisting of only 98 amino acids, used as a genetically encoded fluorescent tag. Previously, only a single fluorogen with an orange color was revealed for this protein. In the present paper, using rational mutagenesis and in vitro screening of fluorogens libraries, we expanded the color palette of this tag. We discovered that E46Q is one of the key substitutions enabling the range of possible fluorogens to be expanded. The introduction of this and several other substitutions has made it possible to use not only orange but also red and green fluorogens with the modified protein.
Computational Modeling of the Interactions between DPP IV and Hemorphins
Authors: Antony Priya , Baby Bincy , Jobe Amie , Vijayan Ranjit
Published: March 2024

Type 2 diabetes is a chronic metabolic disorder characterized by high blood glucose levels due to either insufficient insulin production or ineffective utilization of insulin by the body. The enzyme dipeptidyl peptidase IV (DPP IV) plays a crucial role in degrading incretins that stimulate insulin secretion. Therefore, the inhibition of DPP IV is an established approach for the treatment of diabetes. Hemorphins are a class of short endogenous bioactive peptides produced by the enzymatic degradation of hemoglobin chains. Numerous in vitro and in vivo physiological effects of hemorphins, including DPP IV inhibiting activity, have been documented in different systems and tissues. However, the underlying molecular binding behavior of these peptides with DPP IV remains unknown. Here, computational approaches such as protein–peptide molecular docking and extensive molecular dynamics (MD) simulations were employed to identify the binding pose and stability of peptides in the active site of DPP IV. Findings indicate that hemorphins lacking the hydrophobic residues LVV and VV at the N terminal region strongly bind to the conserved residues in the active site of DPP IV. Furthermore, interactions with these critical residues were sustained throughout the duration of multiple 500 ns MD simulations. Notably, hemorphin 7 showed higher binding affinity and sustained interactions by binding to S1 and S2 pockets of DPP IV.
Evaluating Temperature Effects on Bluetongue Virus Serotype 10 and 17 Coinfection in Culicoides sonorensis
Authors: Carpenter Molly , Kopanke Jennifer , Lee Justin , Rodgers Case , Reed Kirsten , Sherman Tyler J. , Graham Barbara , Cohnstaedt Lee W. , Wilson William C. , Stenglein Mark , Mayo Christie
Published: March 2024

Bluetongue virus (BTV) is a segmented, double-stranded RNA virus transmitted by Culicoides midges that infects ruminants. As global temperatures increase and geographical ranges of midges expand, there is increased potential for BTV outbreaks from incursions of novel serotypes into endemic regions. However, an understanding of the effect of temperature on reassortment is lacking. The objectives of this study were to compare how temperature affected Culicoides survival, virogenesis, and reassortment in Culicoides sonorensis coinfected with two BTV serotypes. Midges were fed blood meals containing BTV-10, BTV-17, or BTV serotype 10 and 17 and maintained at 20 °C, 25 °C, or 30 °C. Midge survival was assessed, and pools of midges were collected every other day to evaluate virogenesis of BTV via qRT-PCR. Additional pools of coinfected midges were collected for BTV plaque isolation. The genotypes of plaques were determined using next-generation sequencing. Warmer temperatures impacted traits related to vector competence in offsetting ways: BTV replicated faster in midges at warmer temperatures, but midges did not survive as long. Overall, plaques with BTV-17 genotype dominated, but BTV-10 was detected in some plaques, suggesting parental strain fitness may play a role in reassortment outcomes. Temperature adds an important dimension to host–pathogen interactions with implications for transmission and evolution.
OPRM1 Gene Polymorphism in Women with Alcohol Use Disorder
Authors: Boroń Agnieszka , Suchanecka Aleksandra , Chmielowiec Krzysztof , Śmiarowska Małgorzata , Chmielowiec Jolanta , Strońska-Pluta Aleksandra , Recław Remigiusz , Grzywacz Anna
Published: March 2024

The main aims of the present study were to explore the relationship of the OPRM1 gene rs1074287 polymorphism in alcohol-dependent women with their personality traits and to try to find out whether any specific features may influence alcohol cravings and be a prognostic for alcohol dependency and treatment in AUD women. Our study found a notable correlation between openness and the interaction of the ORIM1 gene and AUD. The alcohol use disorder subjects with genotype AG showed a higher level of openness compared to the control group with genotypes AG (p = 0.0001) and AA (p = 0.0125). The alcohol use disorder subjects with the AA genotype displayed higher levels of openness than the control group with genotype AG (p = 0.0271). However, the alcohol use disorder subjects with the AA genotype displayed lower levels of openness than the control group with genotype GG (p = 0.0212). Our study indicates that openness as a personality trait is correlated with the OPRM1 gene rs1074287 polymorphism in alcohol-dependent women. These are the first data and results exploring such a relationship between opioid and alcohol pathways and the mental construction of AUD women. Personality traits such as openness to experience and neuroticism might play major roles in the addiction mechanism, especially in genetically predisposed females, independent of the reward system involved in the emotional disturbances that coexist with anxiety and depression.
Molecular World Today and Tomorrow: Recent Trends in Biological Sciences
Authors: Zaman Wajid
Published: March 2024

Various molecular techniques based on omics (transcriptomics, proteomics, genomics) and phylogenetics have been applied in the field of biological sciences [...]
Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
Authors: Prylutska Svitlana , Grebinyk Anna , Ponomarenko Stanislav , Gövem Defne , Chumachenko Vasyl , Kutsevol Nataliya , Petrovsky Mykola , Ritter Uwe , Frohme Marcus , Piosik Jacek , Prylutskyy Yuriy
Published: March 2024

The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology–Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated.
Congenital Optic Disc Anomalies: Insights from Multimodal Imaging
Authors: Cennamo Gilda , Rinaldi Michele , Concilio Marina , Costagliola Ciro
Published: March 2024

In this comprehensive review, we delve into the significance of multimodal imaging in diagnosing and managing complications of congenital optic disc anomalies. While the fundus examination is the gold standard tool in the diagnosis of these pathologies, spectral domain (SD) optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA) could shed light on the pathogenesis and treatment. Moreover, this review seeks to offer a comprehensive insight into the multimodal approach of these rare congenital pathologies. In conclusion, congenital anomalies of the optic nerve represent a major challenge for ophthalmologists. Further research could be useful to clarify the pathophysiology of these diseases and define a correct and more specific treatment approach.
Management of Scar Contractures of the Hand—Our Therapeutic Strategy and Challenges
Authors: Cho Hoyu , Ono Shimpei , Chung Kevin C.
Published: March 2024

The essence of treating scar contractures lies in covering the skin deficit after releasing the contractures, typically using flaps or skin grafts. However, the specific characteristics of scar contractures, such as their location, shape, and size, vary among patients, which makes surgical planning challenging. To achieve excellent outcomes in the treatment of scar contractures, we have developed a dimensional classification system for these contractures. This system categorizes them into four types: type 1 (superficial linear), type 2-d (deep linear), type 2-s (planar scar contractures confined to the superficial layer), and type 3 (planar scar contractures that reach the deep layer, i.e., three-dimensional scar contractures). Additionally, three factors should be considered when determining surgical approaches: the size of the defect, the availability of healthy skin around the defect, and the blood circulation in the defect bed. Type 1 and type 2-d are linear scars; thus, the scar is excised and sutured in a straight line, and the contracture is released using z-plasty or its modified methods. For type 2-s, after releasing the scar contracture band, local flaps are indicated for small defects, pedicled perforator flaps for medium defects, and free flaps and distant flaps for large defects. Type 2-s has good blood circulation in the defect bed, so full-thickness skin grafting is also a suitable option regardless of the defect’s size. In type 3, releasing the deep scar contracture will expose important structures with poor blood circulation, such as tendons, joints, and bones. Thus, a surgical plan using flaps, rather than skin grafts, is recommended. A severity classification and treatment strategy for scar contractures have not yet been established. By objectively classifying and quantifying scar contractures, we believe that better treatment outcomes can be achieved.
Exploring the Factors Affecting Farmers’ Willingness to Cultivate Eco-Agriculture in the Qilian Mountain National Park Based on an Extended TPB Model
Authors: Zhang Mengtian , Wang Huiling
Published: March 2024

Despite many governments having actively promoted the importance of developing ecological agriculture, the participation rate of farmers remains relatively low. Therefore, exploring the factors that influence farmers to participate in agroecological cultivation is important. Here, our aim was to identify the intention to participate in eco-agriculture through an extended theory of the planned behavior model. We collected 409 samples using a systematic probability proportional sampling method in Tianzhu County, located in the Gansu section of the Qilian Mountain National Park, China. The results validated that farmers’ attitudes, perceived behavioral control, and subjective norms positively influenced their intention to participate in agroecology. The extended model introduces ecological value variables; if perceived behavioral control influences willingness to participate through the mediating variable of attitude, ecological values moderate the mediating role of attitude. Agroecological development in national parks should be promoted by strengthening agroecological communication and increasing environmental awareness among farmers. Our study expands the theoretical model of planned behavior, which can help policymakers better understand the factors that influence farmers’ participation in agroecological farming. It can also serve as a reference for the ecological development of agriculture in other protected areas.
Morphology Optimization of Residential Communities towards Maximizing Energy Self-Sufficiency in the Hot Summer Cold Winter Climate Zone of China
Authors: Zhou Yuan , Liu Hongcheng , Xiong Xing , Li Xiaojun
Published: March 2024

Further research is needed on the capability of residential communities to achieve energy self-sufficiency under the constraints of current standards of land use, in particular for the Hot Summer and Cold Winter climate zone (HSCW) of China, where the majority of communities are dominated by high floor-area ratios, thus high-rise dwellings, namely less solar potential per unit floor area, while most residents adopt a “part-time, part-space” pattern of intermittent energy use behavior, thus using relatively low energy per unit floor area. This study examines 150 communities in Changsha to identify morphological indicators and develop a prototype model utilizing the Grasshopper platform. Community morphology is simulated and optimized by taking building location, orientation, and number of floors as independent variables and building energy consumption, solar PV generation, and energy self-sufficiency rate as dependent variables. The results reveal that the morphology optimization can achieve a 4.26% decrease in building energy consumption, a 45% increase in PV generation, and a 13.2% enhancement in energy self-sufficiency, with the optimal being 39%. It highlights that energy self-sufficiency cannot be achieved solely through morphology improvements. Moreover, the study underscores the crucial role of community orientation in maximizing energy self-sufficiency, with the south–north orientation identified as the most beneficial. Additionally, a layout characterized by a horizontally closed and staggered pattern and a vertically scattered arrangement emerges as favorable for enhancing energy self-sufficiency. These findings underscore the importance of considering morphological factors, particularly community orientation, in striving towards energy-self-sufficient high-rise residential communities within the HSCW climate zone of China.
Consumption of Citric Acid by Bees Promotes the Gland Development and Enhances Royal Jelly Quality
Authors: Wang Xue , Ji Quanzhi , Zheng Xing , Zhang Jun , Wang Rongshen , Wang Xinyu , Peng Wenjun , Guo Jun , Zhao Yazhou
Published: March 2024

The glands of bees are responsible for generating and secreting various biologically active substances that significantly impact bee physiological health and adaptability. This study aimed to investigate the effects of adding citric acid (CA) to bee feed on gland development and royal jelly quality. By formulating feed with varying proportions of CA, evaluation was undertaken of pollen feeding by honeybees under laboratory conditions, along with the impact of CA on the development of major glands, to determine suitable addition proportions. Further optimization of the CA proportion involved feeding colonies and evaluating royal jelly production and quality. The results indicated that feed containing 0.75% CA significantly extended the lifespan of bees and increased their pollen consumption. Gland development in bees showed a positive correlation with CA addition within the range of 0.25% to 0.75%, especially at 0.50% and 0.75%, which notably accelerated the development of mandibular, hypopharyngeal, and cephalic salivary glands, with active proliferation and differentiation of glandular cells and maintenance of normal gland size and morphology. CA added to feed stimulated vigorous secretion of wax glands in worker bees, resulting in prolific wax construction. Colonies consuming feed containing 0.50% CA produced royal jelly with significantly reduced moisture and total sugar content and increased levels of 10-HDA, total phenolic acids, total proteins, and acidity. These findings demonstrate that CA consumption significantly prolongs bee lifespan, increases consumption, promotes gland development, and enhances royal jelly quality. This research provides theoretical guidance for beekeeping practices and feed development, contributing to the sustainable advancement of apiculture.
2-((5-(3-(2-Fluorophenyl)acryloyl)-4-methylthiazol-2-yl)amino)isoindoline-1,3-dione
Authors: Fedusevych Olha-Maria , Lozynskyi Andrii , Sulyma Marta , Lesyk Roman
Published: March 2024

In this work, the title compound was synthesized via the Claisen–Schmidt condensation of a 2-((5-acetyl-4-methylthiazol-2-yl)amino)isoindoline-1,3-dione with 2-fluorobenzaldehyde. The structure of the synthesized compound (yield 62%) was confirmed by 1H, 13C NMR, and LC–MS spectra. According to US NCI protocols, the compound displayed a high level of antimitotic activity against tested human tumor cells, with mean GI50/TGI values of 15.72/50.68 μM. The drug-like properties of the synthesized compound were evaluated using SwissAdme, revealing satisfactory drug-like parameters, and it presents interest for the design of new synthetic agents with biological activity.
Influence of Accelerators on Cement Mortars Using Fluid Catalytic Cracking Catalyst Residue (FCC): Enhanced Mechanical Properties at Early Curing Ages
Authors: Soriano Lourdes , Borrachero María Victoria , Giménez-Carbo Ester , Tashima Mauro M. , Monzó José María , Payá Jordi
Published: March 2024

Supplementary cementitious materials (SCMs) have been used in the construction industry to mainly reduce the greenhouse gas emissions associated with Portland cement. Of SCMs, the petrochemical industry waste known as fluid catalytic cracking catalyst residue (FCC) is recognized for its high reactivity. Nevertheless, the binders produced using SCMs usually present low mechanical strength at early curing ages. This study aims to assess the effect of different accelerating additives (KOH, sodium silicate SIL, commercial additive SKR) on the mechanical strength of mortars containing FCC. The results show that after only 8 curing hours, the compressive strength gain of the FCC mortars containing SKR was over 100% compared to the FCC mortar with no additive (26.0 vs. 12.8 MPa). Comparing the compressive strength of FCC mortar containing SKR to the control mortar, the enhancement is spetacular (6.85 vs. 26.03 MPa). The effectiveness of the tested accelerators at 8–24 curing hours was KOH ≈ SIL < SKR, whereas it was KOH < SIL < SKR for 48 h–28 days. The thermogravimetric data confirmed the good compatibility of FCC and the commercial accelerator.
Impact of Welding Parameters in the Porosity of a Dissimilar Welded Lap Joint of CP800-XPF1000 Steel Weldment by GMAW-P
Authors: Garcia-Guerrero Julio Cesar , Curiel-López Francisco Fernando , López-Morelos Víctor Hugo , Taha-Tijerina Jose Jaime , Sánchez-Cruz Teresita Jesus , Ramirez-Lopez Maria del Carmen , Cortes-Carillo Eduardo , Quinones-Salinas Miguel Angel
Published: March 2024

The use of the orthogonal array L4 allows a determination of the effect between the welding parameters peak current (Ip), background current (Ib) and frequency (f) on the porosities in a dissimilar welded lap joint of CP800 and XPF1000 steel weldment by the gas metal arc welding process with the transfer pulsed mode. According to the results, modifications in the welding parameters affect the heat input during welding. A heat input higher than 0.30 KJ/mm generates up to 0.32% porosity in the weld metal, while a heat input lower than 0.25 KJ/mm generates up to 28% porosity in the weld metal. The variation in heat input generated by the process allowed the observation of the final microstructure of the welded joints and the effect of mechanical properties such as hardness because the results show values of hardness from 300 Hv to 400 Hv in the heat affected zone (HAZ).
Identification and Molecular Characterization of Giant Liver Fluke (Fascioloides magna) Infection in European Fallow Deer (Dama dama) in Romania—First Report
Authors: Popovici Dan-Cornel , Dărăbuș Gheorghe , Marin Ana-Maria , Ionescu Ovidiu , Moraru Maria Monica Florina , Imre Mirela , Tîrziu Emil , Mederle Narcisa
Published: March 2024

Fascioloidosis is a parasitic disease of primary wild and domestic ruminants, caused by giant liver fluke, Fascioloides magna. The definitive host of the liver fluke in its area of origin (North America) is the white-tailed deer (Odocoileus virginianus). In Europe, the red deer (Cervus elaphus) and European fallow deer (Dama dama) are definitive hosts and the most sensitive hosts to F. magna infection, on which the parasite exerts serious pathogenic effects. In this study, we analyzed fecal samples and livers of 72 D. dama from 11 hunting grounds in Arad County, Romania. Of the 72 fecal samples and livers from D. dama, trematodes of the genus Fascioloides were identified in four (5.56%). Sequencing revealed that the trematodes identified in the samples were similar to the sequence of F. magna (GenBank no. EF534992.1, DQ683545.1, KU232369.1). The sequence obtained from the molecular analysis has been deposited in GenBank® under accession number OQ689976.1. This study describes the first report of giant liver fluke (F. magna) infection in D. dama in Romania.
A Retrospective Analysis of Salmonella Isolates across 11 Animal Species (1982–1999) Led to the First Identification of Chromosomally Encoded blaSCO-1 in the USA
Authors: Iduu Nneka Vivian , Raiford Donna , Conley Austin , Scaria Joy , Nelson Julie , Ruesch Laura , Price Stuart , Yue Min , Gong Jiansen , Wei Lanjing , Wang Chengming
Published: March 2024

Antimicrobial resistance (AMR) in non-typhoidal Salmonella is a pressing public health concern in the United States, necessitating continuous surveillance. We conducted a retrospective analysis of 251 Salmonella isolates from 11 animal species recovered between 1982 and 1999, utilizing serotyping, antimicrobial susceptibility testing, and whole-genome sequencing (WGS). Phenotypic resistance was observed in 101 isolates, with S. Typhimurium, S. Dublin, S. Agona, and S. Muenster prevailing among 36 identified serovars. Notably, resistance to 12 of 17 antibiotics was detected, with ampicillin being most prevalent (79/251). We identified 38 resistance genes, primarily mediating aminoglycoside (n = 13) and β-lactamase (n = 6) resistance. Plasmid analysis unveiled nine distinct plasmids associated with AMR genes in these isolates. Chromosomally encoded blaSCO-1 was present in three S. Typhimurium and two S. Muenster isolates from equine samples, conferring resistance to amoxicillin/clavulanic acid. Phylogenetic analysis revealed three distinct clusters for these five isolates, indicating evolutionary divergence. This study represents the first report of blaSCO-1 in the USA, and our recovered isolates harboring this gene as early as 1989 precede those of all other reports. The enigmatic nature of blaSCO-1 prompts further research into its function. Our findings highlight the urgency of addressing antimicrobial resistance in Salmonella for effective public health interventions.
Study on the Migration and Release of Sulfur during the Oxidizing Roasting of High-Sulfur Iron Ore
Authors: Chen Xiaojiao , Zhao Na , Li Zhe , Wang Zijian
Published: March 2024

In China, most of the high-sulfur iron ores have not been fully developed and utilized due to the lack of breakthrough progress in the research on the sulfur migration and the desulfurization mechanism during the roasting process. This study will focus on revealing the release and fixation mechanisms of sulfur during the roasting process to achieve the transformation of desulfurization from terminal treatment to process control. Experimental results show that as the roasting temperature increases, the release rate of SO2 also increases, reaching the maximum release rate at 900 °C. Simultaneously, it is found that at the same roasting temperature, the release rate and amount of SO2 under the O2/N2 atmosphere is significantly greater than that under the pure N2 and air atmospheres. Meanwhile, X-ray diffraction (XRD) is utilized to explore the phase composition of the roasted product and the sulfur release mechanism. In addition, the adsorption energy, stability and electron transfer of SO2 on the CaO surface are calculated through density functional theory (DFT), and the optimal adsorption active site perpendicular to the O atom (O-top) is also determined. Finally, the sulfur fixing agent CaO is used to study the SO2 fixation mechanism. When the concentration reaches 10%, the sulfur fixation efficiency reaches more than 80%. Therefore, this work will present basic knowledge and systematic guidance for the sulfur migration and release of high-sulfur iron ore under the oxidizing roasting process.
Biodegradable Metal Complex-Gated Organosilica for Dually Enhanced Chemodynamic Therapy through GSH Depletions and NIR Light-Triggered Photothermal Effects
Authors: Kong Lin , Li Jian , Zhang Yunxiu , Wang Jian , Liang Ke , Xue Xiaokuang , Chen Tiejin , Hao Yongliang , Ren Haohui , Wang Pengfei , Ge Jiechao
Published: March 2024

Hollow silica spheres have been widely studied for drug delivery because of their excellent biosecurity and high porosity. However, difficulties with degradation in the tumor microenvironment (TME) and premature leaking during drug delivery limit their clinical applications. To alleviate these problems, herein, hollow organosilica spheres (HOS) were initially prepared using a “selective etching strategy” and loaded with a photothermal drug: new indocyanine green (IR820). Then, the Cu2+–tannic acid complex (Cu-TA) was deposited on the surface of the HOS, and a new nanoplatform named HOS@IR820@Cu-TA (HICT) was finally obtained. The deposition of Cu-TA can gate the pores of HOS completely to prevent the leakage of IR820 and significantly enhance the loading capacity of HOS. Once in the mildly acidic TME, the HOS and outer Cu-TA decompose quickly in response, resulting in the release of Cu2+ and IR820. The released Cu2+ can react with the endogenous glutathione (GSH) to consume it and produce Cu+, leading to the enhanced production of highly toxic ·OH through a Fenton-like reaction due to the overexpressed H2O2 in the TME. Meanwhile, the ·OH generation was remarkably enhanced by the NIR light-responsive photothermal effect of IR820. These collective properties of HICT enable it to be a smart nanomedicine for dually enhanced chemodynamic therapy through GSH depletions and NIR light-triggered photothermal effects.
A Narrative Review Comparing Nutritional Screening Tools in Outpatient Management of Cancer Patients
Authors: Gil-Andrés Delia , Cabañas-Alite Luis
Published: March 2024

Malnutrition during cancer has a negative impact on prognosis and quality of life. Therefore, it is important to identify those patients at higher nutritional risk to prevent its development. There are nutritional screening tools, such as MUST and NRS-2002, that focus on the patient on admission to hospital. However, most patients will develop malnutrition in the outpatient or ambulatory setting. This study aims to determine which nutritional screening tool is most effective in assessing nutritional risk in the outpatient oncology patient, highlighting the parameters analysed by these tools. Seventeen articles were reviewed, with the most important variables being tumour location, tumour stage, age, and gender, as well as recent weight loss, dietary intake, and digestive disorders. The Nutriscore, NRS-2002, and MUST tools are considered suitable, but the choice varies depending on these parameters. MNA is suitable for elderly patients, while SNAQ was not considered reliable in this population. In conclusion, MUST, NRS-2002, and Nutriscore are suitable tools, but their choice depends on specific characteristics. There is currently no universal tool for nutritional risk assessment in outpatients.
Prevalence, Diversity, and Virulence of Campylobacter Carried by Migratory Birds at Four Major Habitats in China
Authors: Wu Shanrui , Jia Ru , Wang Ying , Li Jie , Li Yisong , Wang Lan , Wang Yani , Liu Chao , Jia Elena M. , Wang Yihua , Zhang Guogang , Liu Jie
Published: March 2024

Campylobacter species, especially C. jejuni and C. coli, are the main zoonotic bacteria causing human gastroenteritis. A variety of Campylobacter species has been reported in wild birds, posing a potential avian–human transmission pathway. Currently, there has been little surveillance data on Campylobacter carriage in migratory birds in China. In the current work, fresh fecal droppings from individual migratory birds were collected at four bird wintering/stopover sites in China from May 2020 to March 2021. Nucleic acid was extracted and tested for Campylobacter with PCR-based methods. Overall, 73.8% (329/446) of the samples were positive for Campylobacter, demonstrating location and bird host specificity. Further speciation revealed the presence of C. jejuni, C. coli, C. lari, C. volucris, and an uncharacterized species, which all harbored a variety of virulence factors. Phylogenetic analysis performed on concatenated 16S rRNA-atpA-groEL genes elucidated their genetic relationship, demonstrating both inter- and intra-species diversity. The wide distribution and high diversity of Campylobacter spp. detected in migratory birds in China indicated potential transmission across territories. The existence of virulence factors in all of these species highlighted their public health importance and the necessity of monitoring and controlling Campylobacter and other pathogens carried by migratory birds.
Enhancing Therapeutic Efficacy of Donepezil, an Alzheimer’s Disease Drug, by Diplazium esculentum (Retz.) Sw. and Its Phytochemicals
Authors: Inthachat Woorawee , Chantong Boonrat , Pitchakarn Pornsiri , Takoon Chawalit , Karinchai Jirarat , Suttisansanee Uthaiwan , Temviriyanukul Piya
Published: March 2024

Alzheimer’s disease (AD) is the most common type of dementia and a significant concern to global public health due to the prevalence of aging populations. Donepezil is one of only a few medications approved for use as an anti-AD agent but all have adverse side effects. Reducing the dosage of AD drugs with plant extracts (phytotherapy) while maintaining efficacy is one strategy to minimize adverse side effects. We previously reported the anti-AD properties of an edible fern, Diplazium esculentum (Retz.) Sw. (DE), which inhibited key enzymes involved in AD pathogenesis including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase 1 (BACE-1). This study aimed to determine whether DE exhibited a synergistic effect with donepezil. The enzyme inhibitory assay showed that DE extract and its bioactive compounds, kaempferol, and quercetin, slightly impeded AChE inhibition with donepezil, while DE extract and quercetin showed synergistic or additive effects with donepezil against BChE and BACE-1, respectively. DE extract combined with donepezil also improved eye phenotypes in a Drosophila model of AD by preventing ommatidia atrophia and bristle breakages. Furthermore, the DE extract exhibited no genotoxic activities, as determined by the Ames test. Our data revealed that DE extract showed promise when combined with donepezil during AD treatment by targeting BChE and BACE-1.
Safety of Dual Orexin Receptor Antagonist Daridorexant: A Disproportionality Analysis of Publicly Available FAERS Data
Authors: Cicala Giuseppe , Barbieri Maria Antonietta , Russo Giulia , Salvo Francesco , Spina Edoardo
Published: March 2024

Daridorexant (dari), as the first dual orexin receptor antagonist (DORA) marketed in Europe, offers a novel therapeutic approach to insomnia. However, data regarding its real-world safety are scarce. Thus, this study was aimed at assessing its safety profile using a large-scale pharmacovigilance database. Dari-related adverse drug reaction (ADR) reports from the Food and Drug Administration Adverse Event Reporting System were scrutinized, and ADRs were selected using reporting odds ratio (ROR) as a measure of disproportionality. Frequencies of events related to dari were compared to all other drugs (reference group, RG1) and only to other DORAs (RG2). Only significant disproportionalities to both RGs were evaluated in-depth. A total of 845 dari-related reports were selected; nightmares (n = 146; dari vs. RG1: ROR = 113.74; 95%CI [95.13, 136]; dari vs. RG2: ROR = 2.35; 95 CI% [1.93, 2.85]), depression (n = 22; dari vs. RG1: 2.13; [1.39, 3.25]; dari vs. RG2: ROR = 2.31; 95 CI% [1.45, 3.67]), and hangover (n = 20; dari vs. RG1: ROR = 127.92; 95 CI% [81.98, 199.62]; and dari vs. RG2: 3.38; [2.04, 5.61]) were considered as safety signals. These data provide valuable insights into the real-world safety profile of daridorexant, supporting the existence of safety signals related to nightmares, depression, and hangovers.
In Vivo Pharmacokinetic Study of Polygonatum cyrtonema Polysaccharide DPC1 after Oral and Intraperitoneal Administration
Authors: Yong Jin , Zhang Chaozheng , Cao Yuening , Tang Shuang , Long Fei , Cao Zhixing , Lu Jun , Peng Teng
Published: March 2024

(1) Background: Polygonatum cyrtonema is a medicinal plant, and its polysaccharides are used for immunomodulation and the treatment of hyperglycemia. Investigation of the tissue distribution and pharmacokinetics of P. cyrtonema polysaccharide can further elucidate its pharmacological mechanisms. (2) Methods: A fluorescence-labeling approach using rhodamine B (RhB) as a fluorescent molecular probe was used for the quantitative assessment of the polysaccharide from dried P. cyrtonema (DPC1) samples, and the pharmacokinetics and tissue distribution of DPC1 were evaluated in mice after intraperitoneal or oral administration. (3) Results: DPC1 was successfully labeled with RhB, showing degrees of fluorescence labeling at 0.453% and 0.568% as determined by the ultraviolet and enzyme marker methods, respectively. DPC1-RhB was rapidly absorbed into the bloodstream after oral and intraperitoneal administration. Pharmacokinetic characteristics showed that oral administration and intraperitoneal administration were consistent with the features of a two-compartment model. (4) Conclusion: After administration, DPC1-RhB was primarily distributed in the tissues of the heart, spleen, and lung, indicating that the drug has a targeted effect on these tissues. Overall, the findings provide a comprehensive reference for the in vivo distribution of DPC1, together with a foundation for further elucidation of its pharmacological mechanisms and the development and application of DPC1 formulations.
Preparation and Characterization of a Novel Salicin–Cyclodextrin Complex
Authors: Căta Adina , Ienaşcu Ioana Maria Carmen , Frum Adina , Ursu Daniel , Svera Paula , Orha Corina , Rusu Gerlinde , Chiș Adriana Aurelia , Dobrea Carmen Maximiliana , Morgovan Claudiu , Pop Oana-Raluca
Published: March 2024

Bioactive compounds extracted from plants can provide wide health benefits. However, some molecules have limited applications as pharmaceuticals due to their limited solubility, poor bioavailability, and low stability when exposed to environmental factors. Their integration in formulations that can deliver them to physiological targets while preserving their biological activity can enhance their usage in improving human health. This research provides a delivery system design to enhance the solubility, stability and to mask the bitter taste of salicin. Thus, a novel salicin-β-cyclodextrin complex was prepared and analyzed by X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, FTIR, Raman and UV-Vis spectroscopy. The analytical and computational methods provided clear and distinct evidence for inclusion of salicin within the β-cyclodextrin cavity and brought important findings for the characterization of the inclusion complex. The present study showed that salicin and β-cyclodextrin can form inclusion complexes, both in solution and in solid state, and that the inclusion of salicin in the cavity of β-cyclodextrin leads to the improvement of its solubility and stability. Thus, the study communicates both qualitative and quantitative knowledge about the preparation of a new salicin-β-cyclodextrin inclusion complex suggesting its potential applications in pharmaceutical industry and medical sciences, as formulations with better compliance for the patient, with increased bioavailability, and easier control of dosage.
Effect of Growth Stage on Nutrition, Fermentation Quality, and Microbial Community of Semidry Silage from Forage Soybean
Authors: Wang Kexin , Sun Shengnan , Zou Yilin , Gao Yongqi , Gao Zifeng , Wang Bo , Hua Yi , Lu Yalin , Hu Guofu , Qin Ligang
Published: March 2024

Soybean (Glycine max (Linn.) Merr.) is highly suitable as animal feed. The silage quality and microbial characteristics of soybean silage are still unclear. Forage soybean (HN389), at six different growth stages (R2-R7), were used as experimental materials to investigate the changes in fermentation, nutritional quality, and microbial characteristics of semidry silage after 0, 7, 14, 30, and 45 d. As the growth period extended, the content of crude protein (CP) and crude fat (EE) gradually increased, while the neutral detergent fiber (NDF) and the acid detergent fiber (ADF) content decreased. The pH value also decreased gradually with fermentation time, accompanied by increases in the proportion of ammonia-N and the content of lactic acid (LA) and acetic acid (AA). In addition, competitive inhibition was observed in the microbial fermentation. With the process of ensiling, Lactobacillus became the dominant bacterial species. The results indicate that the most active stage of fermentation during ensiling occurred within the first 7 days, the fermentation and nutritional quality of the soybean forage were improved, and the optimal mowing stage was the grain stage. Comparison of the microbial abundance showed that all microorganisms entered a stable stage at 30 days of silage. After storage, the dominant bacteria were Lactobacillus, Enterobacter, and Pantoea.
Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants
Authors: Yang Zheng-Chao , Zhao Li-Xiang , Sang Yu-Qi , Huang Xin , Lin Xuan-Chen , Yu Zhi-Ming
Published: March 2024

RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid–liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme.
Research on Physiological Characteristics and Differential Gene Expression of Rice Hybrids and Their Parents under Salt Stress at Seedling Stage
Authors: Zhang Dan , Hu Yuanyi , Li Ruopeng , Tang Li , Mo Lin , Pan Yinlin , Mao Bigang , Shao Ye , Zhao Bingran , Lei Dongyang
Published: March 2024

Soil salinization is one of the most important abiotic stresses which can seriously affect the growth and development of rice, leading to the decrease in or even loss of a rice harvest. Increasing the rice yield of saline soil is a key issue for agricultural production. The utilization of heterosis could significantly increase crop biomass and yield, which might be an effective way to meet the demand for rice cultivation in saline soil. In this study, to elucidate the regulatory mechanisms of rice hybrids and their parents that respond to salt stress, we investigated the phenotypic characteristics, physiological and biochemical indexes, and expression level of salt-related genes at the seedling stage. In this study, two sets of materials, encapsulating the most significant differences between the rice hybrids and their parents, were screened using the salt damage index and a hybrid superiority analysis. Compared with their parents, the rice hybrids Guang-Ba-You-Hua-Zhan (BB1) and Y-Liang-You-900 (GD1) exhibited much better salt tolerance, including an increased fresh weight and higher survival rate, a better scavenging ability towards reactive oxygen species (ROS), better ionic homeostasis with lower content of Na+ in their Na+/K+ ratio, and a higher expression of salt-stress-responsive genes. These results indicated that rice hybrids developed complex regulatory mechanisms involving multiple pathways and genes to adapt to salt stress and provided a physiological basis for the utilization of heterosis for improving the yield of rice under salt stress.
Copper-Containing Bionanocomposites Based on Natural Raw Arabinogalactan as Effective Vegetation Stimulators and Agents against Phytopathogens
Authors: Khutsishvili Spartak S. , Perfileva Alla I. , Kon’kova Tatyana V. , Lobanova Natalya A. , Sadykov Evgeniy K. , Sukhov Boris G.
Published: March 2024

Novel copper-containing bionanocomposites based on the natural raw arabinogalactan have been obtained as universal effective agents against phytopathogen Clavibacter sepedonicus and development stimulants of agricultural plants. Thus, the use of such nanosystems offers a solution to the tasks set in biotechnology while maintaining high environmental standards using non-toxic, biocompatible, and biodegradable natural biopolymers. The physicochemical characteristics of nanocomposites were determined using a number of analytical methods (elemental analysis, transmission electron microscopy and spectroscopic parameters of electron paramagnetic resonance, UV–visible, etc.). The results of the study under the influence of the nanocomposites on the germination of soybean seeds (Glycine max L.) and the vegetation of potatoes (Solanum tuberosum L.) showed the best results in terms of biometric indicators. It is especially worth noting the pronounced influence of the nanocomposite on the development of the root system, and the increase in the mass of the potato root system reached 19%. It is also worth noting that the nanocomposites showed a stimulating effect on the antioxidant system and did not have a negative effect on the content of pigments in potato tissues. Moreover, the resulting bionanocomposite showed a pronounced antibacterial effect against the phytopathogenic bacterium. During the co-incubation of phytopathogen Clavibacter sepedonicus in the presence of the nanocomposite, the number of cells in the bacterial suspension decreased by up to 40% compared to that in the control, and a 10% decrease in the dehydrogenase activity of cells was also detected.