bluuebunny/crossref_metadata_2025 huggingface dataset:
Love beyond gluten: self-esteem, illness identity, and social support in romantic rejection concerns among adolescents with celiac disease
Authors: Shani Maor , van Zalk Maarten H. W.
Published: May 2024

Introduction

Fear of stigmatization, high perceived partner burden, or refraining from self-disclosure may manifest in romantic rejection concerns among adolescents with celiac disease (CD), potentially impacting their health-related quality of life (HRQOL). This study examined the prevalence, predictors, and consequences of romantic rejection concerns among adolescents and young adults with CD.

Methods

A cross-sectional online survey was conducted among 165 German adolescents and young adults (aged 14–22) with self-reported CD. Participants completed measures of romantic rejection concerns, illness identity, self-esteem, peer support, and CD-specific HRQOL.

Results

Participants reported moderate levels of concerns about the impact of CD on their romantic relationships, with no significant gender differences. Participants involved in romantic relationships expressed lower concerns of rejection, but similar preference for a “gluten-free partner.” Hierarchical regression analysis revealed that higher illness acceptance and peer support predicted lower rejection concerns. Significant interactions emerged between self-esteem and illness acceptance, and between self-esteem and peer support. Higher illness acceptance predicted fewer rejection worries only among those with high self-esteem, while peer support played a mitigating role only for those with low self-esteem. Romantic rejection concerns significantly predicted lower CD-specific HRQOL across all domains.

Discussion

Anxieties about CD's impact on romantic relationships are prevalent among adolescents and may hinder their HRQOL. The findings highlight the complex interplay between self-esteem, illness identity, and social support in shaping romantic concerns. Targeted interventions focusing on peer support and fostering positive illness identity are recommended to alleviate rejection fears and improve HRQOL among youth with CD.
Beauty ideals and body positivity: a qualitative investigation of young women’s perspectives on social media content in China
Authors: Lang Min , Ye Yiduo
Published: May 2024

Much of the existing knowledge regarding the impact of beauty ideals and body positive social media content on women’s body image is based on the Western cultural context. This limits our understanding of the issue in other cultures, such as China, among others. Therefore, to address this gap, this study examined young Chinese women’s perspectives on beauty ideals and body positivity in social media through a qualitative investigation. Female university students in China (N = 24) participated in individual interviews. A thematic analysis revealed four primary themes: (1) characteristics of mainstream beauty ideals in Chinese social media; (2) impact of beauty ideals on young women; (3) perspectives on the content and roles of body positivity; (4) influences of body positive social media content on young women. These findings indicate that young Chinese women are aware of the beauty ideals in social media and their negative impact on their body image. Furthermore, young Chinese women generally expressed a favorable outlook on body positivity but noted its limitations.
The mediating effect of self-efficacy on family functioning and psychological resilience in prostate cancer patients
Authors: Zhou Yao , Shan Honglan , Wu Cuigan , Chen Haiyan , Shen Yuanyuan , Shi Wenying , Wang Lina , Li Qinghe
Published: May 2024

Aims

Prostate cancer patients face impaired body image and psychological distress during the diagnosis and treatment of the disease, which leads to changes in mood, cognition and behavior. Psychological resilience has been shown to buffer shocks and stresses from the disease. Therefore, this study investigates the relationship between family functioning and psychological resilience in prostate cancer patients and the mediating role of self-efficacy between family functioning and psychological resilience to provide a relevant theoretical basis for improving patients' psychological status by providing relevant theoretical basis.

Method

Using a cross-sectional design, participants were 215 patients with prostate cancer admitted to and treated in a tertiary hospital in Jiangsu province, China. Questionnaires were administered using the general information questionnaire, the Connor-Davidson Resilience Scale (CD-RISC), the Family Adaptation, Partnership, Growth, Affection, and Resolution Index (APGAR), and the General Self-efficacy Scale (GSES). Data were analyzed using descriptive and correlational analyses and the bootstrap mediation test was used to test the effect relationship between the variables.

Results

Family functioning, self-efficacy and psychological resilience were significantly and positively correlated (r = 0.526, P < 0.01; r = 0.378, P < 0.01; r = 0.358, P < 0.01). The mediating effect of psychological resilience between family functioning and psychological resilience was significant, accounting for 42.56%.

Conclusion

Family function and self-efficacy have been shown to increase the level of psychological resilience in prostate cancer patients. Attention should be paid to the mental health problems of prostate cancer patients, early screening and intervention, and the use of patients' family resources to improve their confidence in recovering from the disease, thus increasing their psychological resilience and improving their mental health.
The interaction effect of high social support and resilience on functional connectivity using seed-based resting-state assessed by 7-Tesla ultra-high field MRI
Authors: Khudeish Nibal , Ramkiran Shukti , Nießen Dominik , Akkoc Altinok Dilsa Cemre , Rajkumar Ravichandran , Dammers Jürgen , Shah N. Jon , Veselinovic Tanja , Neuner Irene
Published: May 2024

Recent resilience research has increasingly emphasized the importance of focusing on investigating the protective factors in mentally healthy populations, complementing the traditional focus on psychopathology. Social support has emerged as a crucial element within the complex interplay of individual and socio-environmental factors that shape resilience. However, the neural underpinnings of the relationship between social support and resilience, particularly in healthy subjects, remain largely unexplored. With advances in neuroimaging techniques, such as ultra-high field MRI at 7T and beyond, researchers can more effectively investigate the neural mechanisms underlying these factors. Thus, our study employed ultra-high field rs-fMRI to explore how social support moderates the relationship between psychological resilience and functional connectivity in a healthy cohort. We hypothesized that enhanced social support would amplify resilience-associated connectivity within neural circuits essential for emotional regulation, cognitive processing, and adaptive problem-solving, signifying a synergistic interaction where strong social networks bolster the neural underpinnings of resilience. (n = 30). Through seed-based functional connectivity analyses and interaction analysis, we aimed to uncover the neural correlates at the interplay of social support and resilience. Our findings indicate that perceived social support significantly (p<0.001) alters functional connectivity in the right and left FP, PCC, and left hippocampus, affirming the pivotal roles of these regions in the brain’s resilience network. Moreover, we identified significant moderation effects of social support across various brain regions, each showing unique connectivity patterns. Specifically, the right FP demonstrated a significant interaction effect where high social support levels were linked to increased connectivity with regions involved in socio-cognitive processing, while low social support showed opposite effects. Similar patterns by social support levels were observed in the left FP, with connectivity changes in clusters associated with emotional regulation and cognitive functions. The PCC’s connectivity was distinctly influenced by support levels, elucidating its role in emotional and social cognition. Interestingly, the connectivity of the left hippocampus was not significantly impacted by social support levels, indicating a unique pattern within this region. These insights highlight the importance of high social support levels in enhancing the neural foundations of resilience and fostering adaptive neurological responses to environmental challenges.
Explaining deep learning-based representations of resting state functional connectivity data: focusing on interpreting nonlinear patterns in autism spectrum disorder
Authors: Kim Young-geun , Ravid Orren , Zheng Xinyuan , Kim Yoojean , Neria Yuval , Lee Seonjoo , He Xiaofu , Zhu Xi
Published: May 2024

Background

Resting state Functional Magnetic Resonance Imaging fMRI (rs-fMRI) has been used extensively to study brain function in psychiatric disorders, yielding insights into brain organization. However, the high dimensionality of the rs-fMRI data presents significant challenges for data analysis. Variational autoencoders (VAEs), a type of neural network, have been instrumental in extracting low-dimensional latent representations of resting state functional connectivity (rsFC) patterns, thereby addressing the complex nonlinear structure of rs-fMRI data. Despite these advances, interpreting these latent representations remains a challenge. This paper aims to address this gap by developing explainable VAE models and testing their utility using rs-fMRI data in autism spectrum disorder (ASD).

Methods

One-thousand one hundred and fifty participants (601 healthy controls [HC] and 549 patients with ASD) were included in the analysis. RsFC correlation matrices were extracted from the preprocessed rs-fMRI data using the Power atlas, which includes 264 regions of interest (ROIs). Then VAEs were trained in an unsupervised manner. Lastly, we introduce our latent contribution scores to explain the relationship between estimated representations and the original rs-fMRI brain measures.

Results

We quantified the latent contribution scores for both the ASD and HC groups at the network level. We found that both ASD and HC groups share the top network connectivitives contributing to all estimated latent components. For example, latent 0 was driven by rsFC within ventral attention network (VAN) in both the ASD and HC. However, we found significant differences in the latent contribution scores between the ASD and HC groups within the VAN for latent 0 and the sensory/somatomotor network for latent 2.

Conclusion

This study introduced latent contribution scores to interpret nonlinear patterns identified by VAEs. These scores effectively capture changes in each observed rsFC feature as the estimated latent representation changes, enabling an explainable deep learning model that better understands the underlying neural mechanisms of ASD.
Depression and anxiety as predictors of performance status and life satisfaction in older adult neurological patients: a cross-sectional cohort study
Authors: Głowacka Mariola , Przybyła Natalia , Humańska Marzena , Kornatowski Maciej
Published: May 2024

Neurological disorders are one of the leading causes of disability globally. Studies emphasise that the course and effectiveness of rehabilitation interventions may be influenced by emotional factors. The aim of the present study was to assess the prevalence of depressive and anxiety symptoms in patients with neurological disorders and examine whether depression and tendency to respond with anxiety are predictors of disability in these patients. The study included 229 individuals with neurological disorders aged over 55. Our findings show that stroke patients are more likely to experience depressive symptoms and are more likely to display trait anxiety as compared with patients with other neurological disorders. Advanced age, female sex, low level of education and the presence of trait anxiety are associated with a higher severity of depressive symptoms. Stroke survivors have significantly poorer ECOG performance scores and are more likely to be incapable for work. Both depression and trait anxiety are significant predictors of neurological patients’ performance status, and the presence of depressive symptoms is a determinant of these patients’ level of satisfaction with life. An understanding of psychological risk factors for poorer performance status in individuals with neurological disorders will make it possible to plan prophylactic interventions in order to minimise negative health impacts and facilitate access to mental health facilities.
Examining associations between physical activity context and children meeting daily physical activity guidelines: the role of outdoor play, sports, and other organized activities
Authors: Pfledderer Christopher D. , Brown Denver M. Y. , Ranjit Nalini , Springer Andrew E. , Malkani Raja I. , Salvo Deborah , Hoelscher Deanna M.
Published: May 2024

Background

Less than one-quarter of US children meet physical activity (PA) guidelines. Understanding the context in which PA occurs and how these contexts may play a role in meeting PA guidelines is an essential step toward developing effective behavioral interventions. The purpose of this study was to examine associations between PA context (sports participation, participation in other organized physical activities, active travel to school, and outdoor play) and the number of days children met PA guidelines in a representative sample of children living in Texas.

Methods

We analyzed cross-sectional data from a statewide sample of fourth-grade children in Texas who completed the 2019–2020 Texas School Physical Activity and Nutrition (Texas SPAN) survey. The Texas SPAN survey was designed to monitor the statewide prevalence of overweight/obesity among school children and assess habitual self-reported obesity-related behaviors, including diet and PA. Weighted Poisson regression models were employed to examine the associations between PA contexts (sports participation, participation in other organized physical activities, active travel to school, and outdoor play) and the number of days children met PA guidelines, adjusting for sex, race/ethnicity, overweight/obesity, urban–rural status, and economic disadvantage.

Results

A total of 16.7% of fourth-grade children met physical activity guidelines every day during the week (mean age = 9.4 ± 0.6 years; female = 48.7, 51.8% Hispanic, mean days meeting PA guideline = 3.6 ± 2.3 days). One in ten (11.2%) children did not meet daily PA guidelines on any day of the week, and 72.1% met them between 1 and 6 days. Participating in sports (b = 0.22, 95%CI:0.14, 0.30), any other organized physical activities (b=0.13, 95%CI:0.017, 0.19), and playing outdoors 1–3 days (b = 0.25, 95%CI:0.04, 0.46) and 4–7 days in the past week (b = 0.77, 95%CI:0.57, 0.97) was significantly and positively associated with the number of days children met PA guidelines.

Conclusion

Participating in sports, participating in other organized physical activities, and playing outdoors may beneficially influence the number of days children meet PA guidelines. PA programs should consider these contextual factors and investigate how to promote organized activities and outdoor play effectively and appropriately among children.
Type 2 diabetes attributable to ambient particulate matter pollution: a global burden study from 1990 to 2019
Authors: Sha Yuyi , Wang Shuai
Published: May 2024

Background

This study assesses the changes over time and geographical locations in the disease burden of type 2 diabetes (T2D) attributed to ambient particulate matter pollution (APMP) from 1990 to 2019 in 204 countries and regions with different socio-demographic indexes (SDI).

Methods

The Global Burden of Diseases Study 2019 (GBD2019) database was used to analyze the global burden of T2D attributed to APMP. This study evaluated both the age-standardized death rate (ASDR) and disability-adjusted life years (DALYs) related to T2D, comparing data from 1990 to 2019. Estimated Annual Percentage Changes (EAPCs) were also utilized to investigate the trends over the 30-year study period.

Results

The global age-standardized DALY rate and ASDR exhibited an increasing trend, with an EAPC of 2.21 (95% CI: 2.15 to 2.27) and 1.50 (95% CI: 1.43 to 1.58), respectively. This rise was most notable among older adult populations, men, regions in Africa and Asia, as well as low-middle SDI regions. In 2019, the ASDR for T2D caused by APMP was recorded at 2.47 per 100,000 population, while the DALY rate stood at 108.98 per 100,000 population. Males and countries with middle SDI levels displayed significantly high age-standardized death and DALY rates, particularly noticeable in Southern Sub-Saharan Africa. Conversely, regions with high SDI levels like High-income North America demonstrated decreasing trends.

Conclusion

This study reveals a significant increase in T2D worldwide as a result of APMP from 1990 to 2019, with a particular emphasis on its impact on men, the older adult, and regions with low to middle SDI levels. These results underscore the urgent necessity for implementing policies aimed at addressing air pollution in order to reduce the prevalence of T2D, especially in the areas most heavily affected.
Correlation between sustainability and smartness indicators in Brazilian cities: insights from the 5SenSu model
Authors: Pierucci Pedro , Agostinho Feni , Almeida Cecília M. V. B. , Demétrio Fernando J. C. , Giannetti Biagio F.
Published: May 2024

To face the growing challenges of urbanization, urban management models grounded in the principles of sustainability and smart cities are being proposed. Despite their acknowledged importance, these models are often misunderstood or improperly applied due to a lack of thorough conceptualization, and they are frequently viewed as interchangeable. The aim of this study is to verify whether a correlation exists between indicators of sustainability and smartness in cities. For a sample of 130 Brazilian cities, their Sustainability Synthetic Indicator of System (SSIS) was computed using the Five-Sector Sustainability Model (5SenSu) and data sourced from the ‘Mandala ODS’ framework. Data concerning smart city were directly sourced from the ‘Connected Smart Cities’ framework. Results show that the 5SenSu model emerges as an alternative multicriteria method, epistemologically grounded in a conceptual model capable of quantifying the sustainability of cities. This approach enables quantitative diagnostics, rankings, and benchmarks, providing information to support decision-making processes. Moreover, the correlation analyses employed reveal a moderate linear correlation (Pearson coefficient of −0.61) and a moderate rank-order correlation (Spearman coefficient of −0.59) between sustainability and smartness. Hence, it would be premature to assume that a city deemed smart would automatically be sustainable, or vice versa. Acknowledging the existing limitations, this study contributes to discussions on the conceptual understanding and quantification through indicators of sustainable and smart cities, providing information for shaping effective public policies aimed at fostering more sustainable urban environments.
Meta-analysis and systematic review of gout prevalence in the heart/lung transplantation population
Authors: Chui Benedict , Day Richard , Umashankar Eshwar , Abdel Shaheed Christina , Keogh Anne , Girgis Laila , Penglase Ross
Published: May 2024

Introduction

Gout may complicate solid organ transplantation with potentially serious consequences. An accurate prevalence of gout in this population is unknown.

Objectives

This study aimed to estimate the prevalence of gout in the heart and/or lung transplantation population through a systematic review and meta-analysis.

Methods

MEDLINE, Embase, PsycINFO, CENTRAL and Cochrane Library (inception to February 2022) were searched for studies that reported the prevalence and/or incidence of gout in heart and/or lung transplant recipients. Two authors extracted outcomes data. Data were pooled using a random effects model. Overall quality of evidence was assessed using GRADE. Primary outcomes were the prevalence of pre- or post-transplant gout expressed as a prevalence rate (95% CI). Secondary outcomes included risk factors for gout, adverse events, and therapeutic complications of gout treatment.

Results

Ten studies were included. Gout prevalence (PR) was 8% pre-transplant (PR = 0.08; 95% CI: 0.05–0.12; 4 studies n = 651) and 6% post-transplant (PR = 0.06; 95% CI: 0.06–0.06; 10 studies n = 45,298). Post-transplant gout prevalence in heart transplant recipients was almost three times higher than lung transplant recipients (PR = 0.16; 95% CI: 0.13–0.20 vs. PR = 0.06; 95% CI: 0.05–0.06 respectively). Patients with a pre-transplant history of gout had a higher risk of developing post-transplant gout than patients without (RR = 3.61; 95% CI: 2.19–5.95). Factors associated with gout and outcomes for heart and/or lung transplant recipients with gout were comprehensively reviewed from the included studies.

Conclusion

Gout is highly prevalent in heart and/or lung transplant patients. Pre-transplant gout is predictive of developing symptomatic post-transplant gout. This has significant implications for management of heart/lung transplant patients.

Systematic Review Registration

https://www.crd.york.ac.uk/, PROSPERO (CRD42020190632).
Kinematics but not kinetics alterations to single-leg drop jump movements following a subject-tailored fatiguing protocol suggest an increased risk of ACL injury
Authors: Nardon Mauro , Ferri Umberto , Caffi Giovanni , Bartesaghi Manuela , Perin Cecilia , Zaza Antonio , Alessandro Cristiano
Published: May 2024

Introduction

Neuromuscular fatigue causes a transient reduction of muscle force, and alters the mechanisms of motor control. Whether these alterations increase the risk of anterior cruciate ligament (ACL) injury is still debated. Here we compare the biomechanics of single-leg drop jumps before and after the execution of a fatiguing exercise, evaluating whether this exercise causes biomechanical alterations typically associated with an increased risk of ACL lesion. The intensity of the fatiguing protocol was tailored to the aerobic capacity of each participant, minimizing potential differential effects due to inter-individual variability in fitness.

Methods

Twenty-four healthy male volunteers performed single leg drop jumps, before and after a single-set fatiguing session on a cycle ergometer until exhaustion (cadence: 65–70 revolutions per minute). For each participant, the intensity of the fatiguing exercise was set to 110% of the power achieved at their anaerobic threshold, previously identified by means of a cardiopulmonary exercise test. Joint angles and moments, as well as ground reaction forces (GRF) before and after the fatiguing exercise were compared for both the dominant and the non-dominant leg.

Results

Following the fatiguing exercise, the hip joint was more extended (landing: Δ=−2.17°, p = 0.005; propulsion: Δ=−1.83°, p = 0.032) and more abducted (landing: Δ=−0.72°, p = 0.01; propulsion: Δ=−1.12°, p = 0.009). Similarly, the knee joint was more extended at landing (non-dominant leg: Δ=−2.67°, p < 0.001; dominant: Δ=−1.4°, p = 0.023), and more abducted at propulsion (both legs: Δ=−0.99°, p < 0.001) and stabilization (both legs: Δ=−1.71°, p < 0.001) hence increasing knee valgus. Fatigue also caused a significant reduction of vertical GRF upon landing (Δ=−0.21 N/kg, p = 0.003), but not during propulsion. Fatigue did not affect joint moments significantly.

Conclusion

The increased hip and knee extension, as well as the increased knee abduction we observed after the execution of the fatiguing exercise have been previously identified as risk factors for ACL injury. These results therefore suggest an increased risk of ACL injury after the execution of the participant-tailored fatiguing protocol proposed here. However, the reduced vertical GRF upon landing and the preservation of joint moments are intriguing, as they may suggest the adoption of protective strategies in the fatigued condition to be evaluated in future studied.
Case report: Chylopericardium secondary to dialysis catheter related jugular venous thrombosis in two dogs receiving long-term hemodialysis
Authors: Woerde Dennis J. , Palm Carrie A. , Duler Laetitia M. , Cowgill Larry D. , Ames Marisa K. , Culp William T. N.
Published: May 2024

Chylopericardium is a rare entity in veterinary medicine. In this report we document the development of chylopericardium in two dogs undergoing chronic hemodialysis. An 11-year-old female spayed Labrador retriever (Case 1) presented with acute coughing and lethargy 2 months following initial dialysis catheter placement and initiation of dialysis therapy for severe azotemia. Echocardiography demonstrated severe pericardial effusion and cardiac tamponade. Pericardial fluid analysis was consistent with chylous effusion. The dog underwent a subtotal pericardiectomy with thoracic duct ligation, and a PleuralPort™ was placed. The patient continued to receive outpatient hemodialysis therapy after pericardiectomy for several months until she died acutely at home. A 4-year-old male neutered Doberman (Case 2) was being treated for 2 months with outpatient hemodialysis for management of chronic kidney disease. On presentation for the 17th hemodialysis treatment, the patient had increased respiratory rate. Echocardiography demonstrated pleural and pericardial effusions, and fluid analysis in both cavities was consistent with chylous effusion. Use of tissue plasminogen activator (TPA), clot removal and replacement of the catheter was attempted; however pleural and pericardial effusion continued. The patient was euthanized after 25 hemodialysis sessions as owners elected not to pursue more procedures. In both cases, the cause of the chylopericardium was suspected to be secondary to catheter-associated thrombosis and/or stenosis based on multiple imaging modalities. Despite use of rivaroxaban and clopidogrel concurrently in each case, the chylous effusion persisted. This case report describes clinical details of a rare complication of long-term indwelling dialysis catheters in two dogs.
Donor Blood Tests do Not Predict Pancreas Graft Survival After Simultaneous Pancreas Kidney Transplantation; a National Cohort Study
Authors: Ho Ning Xuan , Tingle Samuel J. , Malik Abdullah K. , Thompson Emily R. , Kourounis Georgios , Amer Aimen , Pandanaboyana Sanjay , Wilson Colin , White Steve
Published: May 2024

Simultaneous pancreas-kidney (SPK) transplantation improves quality of life and limits progression of diabetic complications. There is reluctance to accept pancreata from donors with abnormal blood tests, due to concern of inferior outcomes. We investigated whether donor amylase and liver blood tests (markers of visceral ischaemic injury) predict pancreas graft outcome using the UK Transplant Registry (2016-2021). 857 SPK recipients were included (619 following brainstem death, 238 following circulatory death). Peak donor amylase ranged from 8 to 3300 U/L (median = 70), and this had no impact on pancreas graft survival when adjusting for multiple confounders (aHR = 0.944, 95% CI = 0.754–1.81). Peak alanine transaminases also did not influence pancreas graft survival in multivariable models (aHR = 0.967, 95% CI = 0.848–1.102). Restricted cubic splines were used to assess associations between donor blood tests and pancreas graft survival without assuming linear relationships; these confirmed neither amylase, nor transaminases, significantly impact pancreas transplant outcome. This is the largest, most statistically robust study evaluating donor blood tests and transplant outcome. Provided other factors are acceptable, pancreata from donors with mild or moderately raised amylase and transaminases can be accepted with confidence. The use of pancreas grafts from such donors is therefore a safe, immediate, and simple approach to expand the donor pool to reach increasing demands.
Experimental Investigation of Solid Rocket Scramjet Based on Central Strut
Authors: Zeng Jia , Wang Guohui , Huang Hui , Fan Jian , Wang Haosu
Published: May 2024

Scramjet based on solid propellant has become a potential choice for the development of future hypersonic vehicles. In this paper, a boron-containing solid rocket scramjet based on the central strut injection was proposed, and the ground direct-connect experiment with the equivalence ratios of 0.43 to 2.4 under the flight condition of Mach 6, 25 km was carried out. The pressure and flow rate over time were measured in the experiment. The results show that the engine can realize stable supersonic mode or subsonic mode combustion by changing the gas flow rate. The engine can effectively increase the combustor pressure, reduce the unstable combustion time, and advance the strong combustion position by increasing the gas flow rate. The engine achieved high combustion efficiency when the equivalence ratio was about 1, with a maximum of 88.28%. A numerical simulation analysis was also carried out in this paper. Compared to the experimental results, the pressure error obtained by numerical simulation was less than 4%, and the typical position error was less than 3%, suggesting that the simulation model can be used to predict the behavior of scramjet.
Genome-Wide Identification and Expression Profiles of IMB Genes Reveal Their Potential Roles in the Gametophytic Sexual Reproduction Process of Camellia sinensis
Authors: Xu Xiaohan , Xing Anqi , Wu Zichen , Sun Yi , Xu Xuefeng , Liu Shujing , Zhao Zhen , Chen Xuan , Li Xinghui , Wang Yuhua
Published: May 2024

It is of great significance to explore the molecular mechanism of gametophytic sexual reproduction in the genetic improvement and breeding of tea plants [Camellia sinensis (L.) O. Kuntze]. Imported beta family members (IMBs) are a class of widely distributed nucleoplasmic transport receptor proteins in eukaryotes, affecting plant development and reproduction, and participating in flowering time and sexual reproduction. Still missing, though, is a thorough examination of IMB members in tea plants. Here, seven members of the IMB gene family were screened by a genome-wide investigation in tea plants. These members were scattered unevenly throughout five chromosomes. All of them contained the conserved KAP95 and HEAT Repeat domains. Additionally, the promoter regions of CsIMBs harbored cis-acting elements associated with plant hormones, light, and abiotic stress responses. In order to further confirm the function of CsIMBs in the sexual reproduction of tea plants, the expression patterns of CsIMBs in different flower development stages and the ovary (before and after pollination) were analyzed. The expression results highlighted that CsIMBs were related to the fertility and fruiting of tea plants. Furthermore, five of the seven CsIMBs (CsIMB1a, CsIMB1b, CsIMB2, CsIMB3a, and CsIMB3b) were found to be localized in the nucleus revealed by subcellular localization analysis. These results offer a comprehensive characterization of IMB genes as well as insights into the potential roles of CsIMBs participating in the gametophytic sexual reproduction of C. sinensis.
Humic Acid Promotes the Growth of Switchgrass under Salt Stress by Improving Photosynthetic Function
Authors: Zhang Jiaxing , Meng Qiuxia , Yang Zhiping , Zhang Qiang , Yan Min , Hou Xiaochan , Zhang Xunzhong
Published: May 2024

As a potential crop in saline-alkali land, the growth of switchgrass could also be threatened by salt stress. Promoting the growth of switchgrass under salt stress by humic acid has great significance in the utilization of saline-alkali land. In this study, a pot experiment was arranged to investigate the responses of photosynthetic and physicochemical characteristics of switchgrass to HA under salt stress. Results showed that humic acid increased the photosynthetic function of switchgrass and enhanced plant height by 41.1% and dry weight by 26.9% under salt stress. Correlation analysis showed that the membrane aquaporin gene PvPIP1, malondialdehyde, ascorbate peroxidase, abscisic acid, polyamine, and jasmonic acid were important factors affecting the photosynthetic function of switchgrass in this study. Meanwhile, HA reduced the content of malondialdehyde, indicating the alleviation of the membrane damage caused by salt stress. On the other hand, HA upregulated the relative expression of the PvPIP1 gene and activated ascorbate peroxidase, abscisic acid, polyamine, and jasmonic acid in switchgrass to resist salt stress. These improved the membrane stability and promoted the photosynthetic activity of switchgrass to enhance the plant’s tolerance against salt stress and growth. Results from this study are helpful to the efficient growing of switchgrass and the sustainable development of saline-alkali land.
Hydrogen Sulfide Increases Drought Tolerance by Modulating Carbon and Nitrogen Metabolism in Foxtail Millet Seedlings
Authors: Zhao Juan , Zhang Shifang , Yang Xiaoxiao , Feng Ke , Wang Guo , Shi Qifeng , Wang Xinru , Yuan Xiangyang , Ren Jianhong
Published: May 2024

Hydrogen sulfide (H2S), a novel gas signaling molecule, has been shown to enhance plant resistance to various abiotic stresses. Here, we investigated the effect of sodium hydrosulfide (NaHS, a H2S donor) on the growth, photosynthetic parameters, and enzyme activities related to carbon and nitrogen metabolism, as well as the levels of carbohydrates and nitrogen metabolites in foxtail millet seedlings subjected to drought stress conditions in pots. The findings revealed that drought stress led to a significant 41.2% decline in the total dry weight (DW) after 12 days of treatment, whereas plants treated with NaHS showed a lesser reduction of 18.7% in total DW. Under drought stress, exogenous NaHS was found to enhance carbon metabolism in foxtail millet seedlings by significantly enhancing photosynthetic capacity, starch, and sucrose content. Additionally, exogenous NaHS was observed to improve nitrogen metabolism by substantially increasing soluble protein content, nitrogen assimilate activity, and synthesis of nitrogen-containing compounds in foxtail millet seedlings. In summary, the exogenous application of NaHS stimulated seedling growth and enhanced drought resistance in foxtail millet by modulating carbon and nitrogen metabolism processes affected by drought stress.
Assessing the Effectiveness of Reflective and Diffusive Polyethylene Films as Greenhouse Covers in Arid Environments
Authors: Al-Madani Abdullah A. , Al-Helal Ibrahim M. , Alsadon Abdullah A.
Published: May 2024

The application of diffusive and reflective polyethylene (PE) films as greenhouse coverings in arid climates presents an opportunity to improve the microclimate of the greenhouse and achieve consistent light distribution within the crop canopy. Nevertheless, there is still a lack of understanding regarding the properties of these covers and their impact on the microclimate and the growth parameters of crops. This study aimed to assess the impact of different covers on the diffusion of beam radiation during transmission, microclimatic parameters, and growth parameters of cucumbers in each of the greenhouses they covered. In the study, three PE covers were evaluated: a reflective cover (RC), a diffusive film (DC), and a locally produced cover (LPC) as the control treatment. The covers were installed on three identical, single-span, evaporatively cooled greenhouses named GH1/LPC, GH2/RC, and GH3/DC, which were utilized for cultivating cucumber crops. The results indicated that the diffusive nature of the tested films increased the ratio of diffuse to global solar radiation (D/G) from 0.22 outside the greenhouses to 0.49, 0.42, and 0.41 inside GH1/LPC, GH3/DC, and GH2/RC, respectively. Similarly, the ratio of diffuse to direct beam radiation (D/B) showed an increase, with values of 0.95, 0.70, and 0.68 inside GH1/LPC, GH3/DC, and GH2/RC, respectively, compared to the outside value of 0.28. The DC used in GH3 showed a favorable microclimate by reducing the air temperature and improving the relative humidity. Accordingly, the vegetative growth of the cucumbers was significantly improved in GH3/DC, reflected in increases in their biomass, followed by GH2/RC and GH1/LPC. The highest crop yield (p ≤ 0.05) of 12.3 kg/m2 was achieved in GH3/DC, followed by 10.2 kg/m2 in GH2/RC and 10.1 kg/m2 in GH1/LPC. Interestingly, the LPC not only stood out as a low-cost option but also displayed excellent diffusive–radiative properties, and demonstrated reasonable growth development and productivity for the cucumber crops. Consequently, the LPC emerges as a practical and cost-effective greenhouse covering material for crop production in arid climates.
Organ Segmentation and Phenotypic Trait Extraction of Cotton Seedling Point Clouds Based on a 3D Lightweight Network
Authors: Shen Jiacheng , Wu Tan , Zhao Jiaxu , Wu Zhijing , Huang Yanlin , Gao Pan , Zhang Li
Published: May 2024

Cotton is an important economic crop; therefore, enhancing cotton yield and cultivating superior varieties are key research priorities. The seedling stage, a critical phase in cotton production, significantly influences the subsequent growth and yield of the crop. Therefore, breeding experts often choose to measure phenotypic parameters during this period to make breeding decisions. Traditional methods of phenotypic parameter measurement require manual processes, which are not only tedious and inefficient but can also damage the plants. To effectively, rapidly, and accurately extract three-dimensional phenotypic parameters of cotton seedlings, precise segmentation of phenotypic organs must first be achieved. This paper proposes a neural network-based segmentation algorithm for cotton seedling organs, which, compared to the average precision of 75.4% in traditional unsupervised learning, achieves an average precision of 96.67%, demonstrating excellent segmentation performance. The segmented leaf and stem point clouds are used for the calculation of phenotypic parameters such as stem length, leaf length, leaf width, and leaf area. Comparisons with actual measurements yield coefficients of determination R2 of 91.97%, 90.97%, 92.72%, and 95.44%, respectively. The results indicate that the algorithm proposed in this paper can achieve precise segmentation of stem and leaf organs, and can efficiently and accurately extract three-dimensional phenotypic structural information of cotton seedlings. In summary, this study not only made significant progress in the precise segmentation of cotton seedling organs and the extraction of three-dimensional phenotypic structural information, but the algorithm also demonstrates strong applicability to different varieties of cotton seedlings. This provides new perspectives and methods for plant researchers and breeding experts, contributing to the advancement of the plant phenotypic computation field and bringing new breakthroughs and opportunities to the field of plant science research.
Assessment of the Impact of Magnesium and Nitrogen Fertilization on Two Species of Grasses Used as Horse Feed
Authors: Sulewska Hanna , Ratajczak Karolina , Roszkiewicz Roman
Published: May 2024

The aim of this study was to determine the effect of nitrogen (three doses of N) and magnesium (two doses of Mg) fertilization on the yield and quality of fodder obtained from two old and extensive grass species Festulolium braunii cv. ‘Felopa’ and Lolium multiflorum cv. ‘Tur’ in field cultivation under dry conditions. F. braunii was better adapted to cultivation on light, dry soils than L. multiflorum; in such conditions, it produces higher yields of dry matter and protein, characterized by a higher concentration of nutrients. F. braunii fertilized with doses of 120 and 180 kg N∙ha−1 yielded higher than that fertilized with a dose of 60 kg N∙ha−1, and L. multiflorum produced similar yields after applying doses of 60, 120 and 180 kg N∙ha−1. For tested grass pasture, a single N application after the start of vegetation in two forms (fast- and slow-acting) appears to be adequate. Resignation from splitting the nitrogen dose due to variable rainfall distribution that can occur after the first cut during the dry summer is beneficial due to a reduction in the losses of nutrients and environmental burdens.
Risk Factors for Therapeutic Failure and One-Year Mortality in Patients with Intramedullary Nail-Associated Infection after Trochanteric and Subtrochanteric Hip Fracture Repair
Authors: Pfang Bernadette , Villegas García Marco A. , Blanco García Antonio , Auñón Rubio Álvaro , Esteban Jaime , García Cañete Joaquín
Published: May 2024

Despite the implications of trochanteric and subtrochanteric intramedullary (IM) nail infection for patients with hip fracture, little is known about risk factors for therapeutic failure and mortality in this population. We performed a retrospective observational analysis including patients diagnosed with trochanteric and subtrochanteric IM nail infection at a Spanish academic hospital during a 10-year period, with a minimum follow-up of 22 months. Of 4044 trochanteric and subtrochanteric IM nail implants, we identified 35 cases of infection during the study period (0.87%), 17 of which were chronic infections. Patients with therapeutic failure (n = 10) presented a higher average Charlson Comorbidity Index (CCI) (5.40 vs. 4.21, p 0.015, CI 0.26–2.13) and higher rates of polymicrobial (OR 5.70, p 0.033, CI 1.14–28.33) and multidrug-resistant (OR 7.00, p 0.027, CI 1.24–39.57) infections. Upon multivariate analysis, polymicrobial infection and the presence of multidrug-resistant pathogens were identified as independent risk factors for therapeutic failure. Implant retention was associated with an increased risk of failure in chronic infection and was found to be an independent risk factor for overall one-year mortality in the multivariate analysis. Our study highlights the importance of broad-spectrum empirical antibiotics as initial treatment of trochanteric and subtrochanteric IM nail-associated infection while awaiting microbiological results. It also provides initial evidence for the importance of implant removal in chronic IM-nail infection.
Whooping Cough Cases Increase in Central Italy after COVID-19 Pandemic
Authors: Linardos Giulia , Coltella Luana , Ranno Stefania , Di Maio Velia Chiara , Colagrossi Luna , Pandolfi Elisabetta , Chiarini Testa Maria Beatrice , Genuini Leonardo , Stoppa Francesca , Di Nardo Matteo , Grandin Annalisa , Cutrera Renato , Cecchetti Corrado , Villani Alberto , Raponi Massimiliano , Bernaschi Paola , Russo Cristina , Perno Carlo Federico , Scutari Rossana
Published: May 2024

Pertussis continues to be a highly contagious respiratory infection, especially in children, with cyclical peaks of disease spread every three to five years. Here, we report relevant cases of B. pertussis infection between August 2023 and January 2024, and compare them with B. pertussis prevalence in pediatric patients admitted to the Reference Italian Pediatric Hospital, located in Rome, from January 2015 to July 2023. A total of 5464 tests for B. pertussis were performed during the study period, and 6.9% were positive. At the time of the COVID-19 pandemic, there was a sharp decrease in the presence of B. pertussis, which reappeared only in August 2023, recording five new cases. All five children presented with paroxysmal cough 5 to 10 days before admission. Four patients had other mild respiratory symptoms and moderate B. pertussis DNA levels (Ct mean: 26). Only one child, with very high B. pertussis DNA levels (Ct: 9), presented with severe respiratory failure. The patients with mild/moderate infection achieved clinical recovery while the patient with the severe manifestation died of cardiac arrest. These observations highlight the reemergence of pertussis even in vaccinated countries and its association with morbidity and mortality especially in young children. This emphasizes the importance of rapid diagnosis to immediately implement appropriate treatment and monitoring of immune status.
Efficacy of Expired Antibiotics: A Real Debate in the Context of Repeated Drug Shortages
Authors: Davido Benjamin , Michelon Hugues , Mamona Christel , de Truchis Pierre , Jaffal Karim , Saleh-Mghir Azzam
Published: May 2024

This narrative review aims to discuss the main interest in and cautions associated with the use of expired antibiotics in the context of repeated shortages, notably in Europe. Articles concerning the topic of expiry dates related to antibiotic use were reviewed using keywords in the PubMed®/MEDLINE and Google Scholar databases to identify the most extensive evidence-based documentation. The present review evaluates the potential interest and efficacy of using expired drugs and their possible related adverse events. Overall, in the context of drug shortages, expiry dates could be safely extended for at least one year for most solid antibiotics (tablets or powder) used in daily clinical practice, as long as they are stored under the right conditions, in accordance with the summary of product characteristics.
Antimicrobial Use Survey and Detection of ESBL-Escherichia coli in Commercial and Medium-/Small-Scale Poultry Farms in Selected Districts of Zambia
Authors: Sinyawa Taona , Shawa Misheck , Muuka Geoffrey M. , Goma Fusya , Fandamu Paul , Chizimu Joseph Yamweka , Khumalo Cynthia Sipho , Mulavu Malala , Ngoma Masuzyo , Chambaro Herman Moses , Kamboyi Harvey Kakoma , Kajihara Masahiro , Sawa Hirofumi , Suzuki Yasuhiko , Higashi Hideaki , Mainda Geoffrey , Munyeme Musso , Muma John Bwalya , Nyantakyi Christian Owusu , Egyir Beverly , Hang’ombe Bernard Mudenda
Published: May 2024

Antimicrobial resistance (AMR) among Escherichia coli from food animals is a rising problem, and heavy antimicrobial use in poultry is a contributing factor. In Zambia, studies linking poultry-associated AMR and antibiotic use (AMU) are rare. This study aimed to investigate commercial and medium-/small-scale poultry farmers’ usage of antimicrobials based on a questionnaire survey in ten districts of Zambia. In addition, the study characterized extended-spectrum β-lactamase (ESBL)-producing E. coli isolates obtained from poultry in the same districts. Data regarding knowledge and usage of antimicrobials were collected from commercial and medium-/small-scale poultry farmers using a pre-tested structured questionnaire. At the same time, cloacal samples were collected and analyzed. One hundred and fifty E. coli isolates were tested for antimicrobial susceptibility using eight antibiotic classes. The isolates were further screened for ESBL production by streaking them on cefotaxime (CTX)-supplemented MacConkey agar, then subjecting them to sequencing on a NextSeq. The questionnaire survey showed that more medium-/small-scale than commercial poultry farmers used antimicrobials (OR = 7.70, 95% CI = 2.88–20.61) but less prescriptions (OR = 0.02, 95% CI = 0.00–0.08). Susceptibility testing revealed that resistance was highest to ampicillin (128/148, 86.5%) and tetracycline (101/136, 74.3%) and that the prevalence of multidrug resistance (MDR) (28/30, 93.3%) was high. Whole-genome sequencing (WGS) of eight (8/30, 26.7%) isolates with CTX Minimum Inhibitory Concentration (MIC) ≥ 4 µg/mL revealed the presence of ESBL-encoding genes blaCTX-M-14, blaCTX-M-55, and blaTEM. WGS also detected other AMR genes for quinolones, aminoglycosides, phenicols, tetracycline, macrolides, and folate-pathway antagonists. Altogether, the questionnaire survey results showed a higher proportion of AMU and lower prescription usage among medium-/small-scale farmers. In addition, our results emphasize the circulation of ESBL-producing E. coli strains with associated MDR. It is critical to educate farmers about AMR risks and to encourage responsible usage of antimicrobials. Furthermore, there is a need to strengthen regulations limiting access to antimicrobials. Finally, there is a need to establish a one health system to guide public health response.