bluuebunny/crossref_metadata_2025 huggingface dataset:
Intra-amniotic inflammation and birth weight in pregnancies with preterm labor with intact membranes: A retrospective cohort study
Authors: Matulova Jana , Kacerovsky Marian , Bolehovska Radka , Kukla Rudolf , Bostik Pavel , Kolarova Klara , Frydrychová Simona , Jacobsson Bo , Musilova Ivana
Published: November 2022

Objective

To assess the association between newborn birth weight and the presence of intra-amniotic infection, presence of sterile intra-amniotic inflammation, and absence of intra-amniotic inflammation in pregnancies with preterm labor with intact membranes.

Methods

A total of 69 pregnancies with preterm labor with intact membranes between gestational ages 22 + 0 and 34 + 6 weeks who delivered within seven days of admission were included in this retrospective cohort study. Transabdominal amniocentesis to determine the presence of microorganisms and/or their nucleic acids in amniotic fluid (through culturing and molecular biology methods) and intra-amniotic inflammation (according to amniotic fluid interleukin-6 concentrations) were performed as part of standard clinical management. The participants were further divided into three subgroups: intra-amniotic infection (presence of microorganisms and/or nucleic acids along with intra-amniotic inflammation), sterile intra-amniotic inflammation (intra-amniotic inflammation alone), and without intra-amniotic inflammation. Birth weights of newborns were expressed as percentiles derived from the INTERGROWTH-21st standards for (i) estimated fetal weight and (ii) newborn birth weight.

Results

No difference in birth weights, expressed as percentiles derived from the standard for estimated fetal weight, was found among the women with intra-amniotic infection, with sterile intra-amniotic inflammation, and without intra-amniotic inflammation (with infection, median 29; with sterile inflammation, median 54; without inflammation, median 53; p = 0.06). Differences among the subgroups were identified in the birth weight rates, expressed as percentiles derived from the standard for estimated fetal weight, which were less than the 10th percentile (with infection: 20%, with inflammation: 13%, without inflammation: 0%; p = 0.04) and 25th percentile (with infection: 47%, with inflammation: 31%, without inflammation: 9%; p = 0.01). No differences among the subgroups were observed when percentiles of birth weight were derived from the birth weight standard.

Conclusions

The presence of intra-amniotic inflammatory complications in pregnancies with preterm labor with intact membranes prior to the gestational age of 35 weeks was associated with a higher rate of newborns with birth weight less than the 10th and 25th percentile, when percentiles of birth weight were derived from the standard for estimated fetal weight.
Clinical profile and treatment outcome of acute intussusception among children in eastern Ethiopia: A seven years retrospective study
Authors: Tesfaye Dawit Desta , Adem Burka Mohammed , Ketema Indeshaw , Mehadi Ame , Eshetu Bajrond , Teshager Tilahun , Asfaw Henock , Desalew Assefa
Published: November 2022

Background

Acute intussusception is the main cause of abdominal surgical emergencies worldwide in young children, with an incidence of approximately 1 to 4 per 2,000 children. An accurate estimate of the treatment outcomes of acute intussusception in children is unknown in low-and middle-income countries like Ethiopia. Hence, this study aimed to determine the clinical profile, treatment outcomes of acute intussusception and its associated factors among children admitted to Hiwot Fana Specialized University Hospital in eastern Ethiopia.

Methods

An institutional-based retrospective cross-sectional study was conducted from November 01 to 30, 2021, among children admitted and managed for acute intussusception. All medical records of children admitted and managed for acute intussusception at Hiwot Fana Specialized University hospital between January 2014 and December 2020 were included. Data were collected using pretested structured checklists through a review of medical records, entered and analyzed using Statistical Package for Social Sciences version 25.0. Chi-square (χ2) tests were applied to determine the associated factors with treatment outcome. The statistical significance was considered at a p-value < 0.05.

Results

In this chart review of children, 13.3% (95% CL: 11.8–14.8) died. The median age of the study participant was 13 months. The majority, 72% were male and 76% were less than 24 months old. Regarding the clinical profile; abdominal pain (94.7%), vomiting (93.3%), bloody diarrhea (70.7%), and abdominal distention (76.0%) were the most common clinical presentations. Age less than 24 months [X2 = 8.13 (df = 1); p = 0.004], preoperative vital signs [X2 = 19.21 (df = 2); p = 0.000], intraoperative findings [X2 = 18.89 (df = 1); p = 0.000], and postoperative complications [X2 = 14.60 (df = 1); p = 0.000] were significantly associated with treatment outcome of acute intussusception.

Conclusion

In this chart review, the overall mortality rate in children was relatively high. One in seven children died from acute intussusception. Age less than 24 months, preoperative vital signs, intraoperative findings, and postoperative complications were significantly associated with acute intussusception treatment outcomes. Surgical management was the only treatment performed in all cases. Delayed presentation of patients and lack of other treatment modalities such as non-surgical interventions are serious concerns in this facility. The initiation of non-surgical reduction may reduce the need for surgical intervention-related complications, and child mortality.
Durable response to the combination of pembrolizumab and nab-paclitaxel in a metastatic extrahepatic cholangiocarcinoma: A case report and literature review
Authors: Tan Sirui , Yu Jing , Huang Qiyue , Zhou Nan , Xiong Xianze , Gou Hongfeng
Published: November 2022

Background: Cholangiocarcinoma (CCA) is a highly aggressive malignant tumor with poor overall survival. Although the first-line standard chemotherapy (gemcitabine plus cisplatin) combined with immunotherapy has yielded positive results with survival prolongation, the efficacy remains unsatisfactory, and new treatment modalities need to be explored.

Case presentation: We report the case of a patient with metastatic extrahepatic CCA who achieved a durable response and good tolerance to the combination treatment of pembrolizumab and nab-paclitaxel following progression on gemcitabine plus capecitabine chemotherapy. The tumor samples of the patient revealed low TMB, MSS, negative PD-L1 expression, and negative CD8+ TIL expression. This patient was treated with 3 cycles of pembrolizumab plus nab-paclitaxel and cisplatin, followed by 5 cycles of pembrolizumab plus nab-paclitaxel. Finally, 10 cycles of pembrolizumab monotherapy were administered. The patient survived for over 27 months after the initiation of combined therapy and was still in continuous remission at the last follow-up.

Conclusion: As far as we know, this is the first report that pembrolizumab plus nab-paclitaxel successfully treated a patient with advanced CCA. This combination therapy might be a potential treatment option for patients with cholangiocarcinoma, and further clinical trials are needed to explore the outcomes.
Adolescent kratom exposure affects cognitive behaviours and brain metabolite profiles in Sprague-Dawley rats
Authors: Zul Aznal Aiman Nadhirah , Mohamad Nor Hazalin Nurul Aqmar , Hassan Zurina , Mat Noorul Hamizah , Chear Nelson Jeng-Yeou , Teh Lay Kek , Salleh Mohd Zaki , Suhaimi Farah Wahida
Published: November 2022

Adolescence is a critical developmental period during which exposure to psychoactive substances like kratom (Mitragyna speciosa) can cause long-lasting deleterious effects. Here, we evaluated the effects of mitragynine, the main alkaloid of kratom, and lyophilised kratom decoction (LKD) on cognitive behaviours and brain metabolite profiles in adolescent rats. Male Sprague-Dawley rats (Postnatal day, PND31) were given vehicle, morphine (5 mg/kg), mitragynine (3, 10, or 30 mg/kg), or LKD (equivalent dose of 30 mg/kg mitragynine) for 15 consecutive days. Later, a battery of behavioural testing was conducted, brain was extracted and metabolomic analysis was performed using LCMS-QTOF. The results showed that mitragynine did not affect the recognition memory in the novel object recognition task. In the social interaction task, morphine, mitragynine, and LKD caused a marked deficit in social behaviour, while in Morris water maze task, mitragynine and LKD only affected reference memory. Metabolomic analysis revealed distinct metabolite profiles of animals with different treatments. Several pathways that may be involved in the effects of kratom exposure include arachidonic acid, pantothenate and CoA, and tryptophan pathways, with several potential biomarkers identified. These findings suggest that adolescent kratom exposure can cause cognitive behavioural deficits that may be associated with changes in the brain metabolite profiles.
The safety and efficacy of oxaliplatin-loaded drug-eluting beads transarterial chemoembolization for the treatment of unresectable or advanced lung cancer
Authors: Bi Yonghua , Li Fazhong , Ren Jianzhuang , Han Xinwei
Published: November 2022

Aim: Drug-eluting beads are usually applied for the treatment of advanced hepatocellular carcinoma. Oxaliplatin was suggested as first-line therapy for advanced non–small-cell lung cancer. However, there has been little investigation about the application of drug-eluting beads transarterial chemoembolization (DEB-TACE) with oxaliplatin-loaded CalliSpheres beads (CB) for the treatment of unresectable or advanced lung cancer. We aimed to investigate the safety and efficacy of oxaliplatin-loaded DEB-TACE for the treatment of unresectable or advanced lung cancer.

Methods: From January 2019 to December 2021, all patients with primary unresectable or advanced lung cancer who underwent DEB-TACE with oxaliplatin-loaded CB were retrospectively enrolled. This study defined overall survival and objective response rate (ORR) as the primary endpoints, disease control rate (DCR) and progression-free survival (PFS) as the secondary endpoints.

Results: A total of 33 sessions of DEB-TACE were performed in 20 patients, with a mean of 1.7 ± 1.0 sessions. A total of 55 arteries were emoblized by CB, including 40 bronchial arteries, 13 intercostal arteries, one suprarenal artery and one inferior phrenical artery. No procedural-related mortality or severe complications were observed. The median tumor diameter was 49.0 [Interquartile range (IQR) 37.8–66.8] mm before DEB-TACE, and decreased to 38.8 (IQR 27.7–56.9), 26.1 (IQR 19.1–48.8), and 20.5 (IQR 13.1–49.7) mm at 1, 3 and 6 months later (p = 0.04). The ORR and DCR at 1, 3, and 6 months after DEB-TACE were 28.6% and 92.9%, 38.5% and 84.6%, 30.8% and 61.5%, respectively. The median PFS and median overall survival was 9.9 and 29.6 months, respectively.

Conclusion: DEB-TACE with oxaliplatin-loaded CB is suggested as a safe, effective and well-tolerated treatment for patients with unresectable or advanced lung cancer.
Effect of M6A regulators on diagnosis, subtype classification, prognosis and novel therapeutic target development of idiopathic pulmonary fibrosis
Authors: Huang Guirui , Huang Shuaiyang , Cui Hongsheng
Published: November 2022

Molecular biology studies show that RNA N6-methyladenosine (m6A) modifications may take part in the incidence and development of idiopathic pulmonary fibrosis (IPF). Nonetheless, the roles of m6A regulators in IPF are not fully demonstrated. In this study, 12 significant m6A regulators were filtered out between healthy controls and IPF patients using GSE38958 dataset. Random forest algorithm was used to identify 11 candidate m6A regulators to predict the incidence of IPF. The 11 candidate m6A regulators included leucine-rich PPR motif-containing protein (LRPPRC), methyltransferase-like protein 3, FTO alpha-ketoglutarate dependent dioxygenase (FTO), methyltransferase-like 14/16, zinc finger CCCH domain-containing protein 13, protein virilizer homolog, Cbl proto-oncogene like 1, fragile X messenger ribonucleoprotein 1 and YTH domain containing 1/2. A nomogram model was constructed based on 11 candidate m6A regulators and considered beneficial to IPF patients using decision curve analysis. Consensus clustering method was used to distinctly divide IPF patients into two m6A patterns (clusterA and clusterB) based on 12 significant m6A regulators. M6A scores of all IPF patients were obtained using principal component analysis to quantify the m6A patterns. Patients in clusterB had higher m6A scores than those in clusterA. Furthermore, patients in clusterB were correlated with Th17 and Treg cell infiltration, innate immunity and Th1 immunity, while those in clusterA were correlated with adaptive immunity and Th2 immunity. Patients in clusterB also had higher expressions of mesenchymal markers and regulatory factors of fibrosis but lower expressions of epithelial markers. Lastly and interestingly, two m6A regulators, LRPPRC (p = 0.011) and FTO (p = 0.042), were identified as novel prognostic genes in IPF patients for the first time using an external GSE93606 dataset. Both of them had a positive correlation with a better prognosis and may serve as therapy targets. Thus, we conducted virtual screening to discover potential drugs targeting LRPPRC and FTO in the treatment of IPF. In conclusion, m6A regulators are crucial to the onset, development and prognosis of IPF. Our study on m6A patterns may provide clues for clinical diagnosis, prognosis and targeted therapeutic drugs development for IPF.
The behaviors of bubble migration and pressure build-up during a dynamic shut-in procedure in deep-water drilling
Authors: Miao Dianyuan , Hu Junjie , Zhang Jianbo , Wang JinTang , Sun Xiaohui
Published: November 2022

A dynamic shut-in procedure is commonly adopted after a kick incident in order to build up the wellbore pressure, obtain reservoir information, and thereby handle the gas kick. In deep-water scenarios, the hydrate growth behaviors have a significant effect on gas migration and interphase mass transfer, which has not been quantitatively analyzed during the well shut-in process. In this study, a comprehensive mechanistic model of wellbore dynamics is developed considering gas migration and phase transitions. The simulation results show that the wellbore pressure field can be built up in different trends during different well shut-in periods, governed by gas seepage from the reservoir and gas migration along the wellbore, respectively. Masking the migration of free gas, the phase transition phenomena have a significant influence on the wellbore dynamics and bottomhole pressure. This work adds further insights into quantitatively characterizing the hydrate growth behaviors and interphase mass transfer rules of gas bubbles during a dynamic well shut-in procedure.
Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation
Authors: Yadav Archana , Mudoi Kalpataru Dutta , Kumar Niraj , Geed Sachin Rameshrao , Gogoi Parishmita , Sharma Rabin K. , Saikia Ratul
Published: November 2022

Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin.
Processes and mechanisms of vegetation ecosystem responding to climate and ecological restoration in China
Authors: Chen Tiantian , Wang Qiang , Wang Yuxi , Peng Li
Published: November 2022

Vegetation is an essential component of the earth’s surface system and its dynamics is a clear indicator of global climate change. However, the vegetation trends of most studies were based on time-unvarying methods, cannot accurately detect the long-term nonlinear characteristics of vegetation changes. Here, the ensemble empirical mode decomposition and the Breaks for Additive Seasonal and Trend algorithm were applied to reconstruct the the normalized difference vegetation index (NDVI) data and diagnose spatiotemporal evolution and abrupt changes of long-term vegetation trends in China during 1982–2018. Residual analysis was used to separate the influence of climate and human activities on NDVI variations, and the effect of specific human drivers on vegetation growth was obtained. The results suggest that based on the time-varying analysis, high vegetation browning was masked by overall vegetation greening. Vegetation growth in China experienced an abrupt change in the 1990s and 2000s, accounting for 50% and 33.6% of the whole China respectively. Of the area before the breakpoint, 45.4% showed a trend of vegetation decrease, which was concentrated mainly in east China, while 43% of the area after the breakpoint also showed vegetation degradation, mainly in northwest China. Climate was an important driving force for vegetation change in China. It played a positive role in south China, but had a negative effect in northwest China. The impact of human activities on vegetation growthchanged from an initial negative influence to a positive one. In terms of human activities, an inverted-U-shaped relation was detected between CO2 emissions and vegetation growth; that is, the fertilization effect of CO2 had a certain threshold. Once that threshold was exceeded, it would hinder vegetation growth. Population density had a slight constraint on vegetation growth, and the implementation of ecological restoration projects (e.g., the Grain for Green Program) can promote vegetation growth to a certain extent.
Silicon inhibits gummosis by promoting polyamine synthesis and repressing ethylene biosynthesis in peach
Authors: Gao Huaifeng , Wu Xuelian , Yang Xiaoqing , Sun Maoxiang , Liang Jiahui , Xiao Yuansong , Peng Futian
Published: November 2022

Silicon is a beneficial element for plant growth, as well as for improving plant resistance to multiple biotic and abiotic stresses. Gummosis is a common harmful disease in peach and is induced by many factors. However, the effect of silicon on gummosis of peach has not been determined yet. In this study, we reported that application of silicon significantly reduced gummosis by regulating biosynthesis of ethylene and polyamines in peach. Ethylene promoted the development of gummosis by inducing the expression of genes encoding cell wall degrading enzymes. While application of different types of polyamines, including spermidine and spermine, dramatically inhibited the occurrence of gummosis. Moreover, polyamines inhibited the ethylene biosynthesis by down-regulating expression of ethylene biosynthetic gene PpACS1 (1-aminocyclopropane -1-carboxylic acid synthase), as well as the enzymatic activity of ACS. We further found that application of silicon significantly restricted the development of gummosis in peach. Exogenous silicon dramatically inhibited expression of PpACS1 and the enzymatic activity of its product to reduce ethylene biosynthesis. Simultaneously, the activity of S-adenosylmethionine decarboxylase, a key enzyme in ployamines biosynthesis, was increased by 9.85% under silicon treatment, resulting in elevated accumulation of polyamines. Thus, our data proved that application of silicon restricted gummosis development by activating ployamines biosynthesis and inhibiting ethylene synthesis in peach.
Diverse mechanisms associated with cyhalofop-butyl resistance in Chinese sprangletop (Leptochloa chinensis (L.) Nees): Characterization of target-site mutations and metabolic resistance-related genes in two resistant populations
Authors: Zhang Yi , Chen Liping , Song Wen , Cang Tao , Xu Mingfei , Wu Changxing
Published: November 2022

Resistance of Chinese sprangletop (Leptochloa chinensis (L.) Nees) to the herbicide cyhalofop-butyl has recently become a severe problem in rice cultivation. However, the molecular mechanisms of target-site resistance (TSR) in cyhalofop-butyl-resistant L. chinensis as well as the underlying non-target-site resistance (NTSR) have not yet been well-characterized. This study aimed to investigate cyhalofop-butyl resistance mechanisms using one susceptible population (LC-S) and two resistant populations (LC-1701 and LC-1704) of L. chinensis. We analyzed two gene copies encoding the entire carboxyltransferase (CT) domain of chloroplastic acetyl-CoA carboxylase (ACCase) from each population. Two non-synonymous substitutions were detected in the resistant L. chinensis populations (Trp2027-Cys in the ACCase1 of LC-1701 and Leu1818-Phe in the ACCase2 of LC-1704), which were absent in LC-S. As Trp2027-Cys confers resistance to ACCase-inhibiting herbicides, the potential relationship between the novel Leu1818-Phe mutation and cyhalofop-butyl resistance in LC-1704 was further explored by single-nucleotide polymorphism (SNP) detection. Metabolic inhibition assays indicated that cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs) contributed to cyhalofop-butyl resistance in specific resistant populations. RNA sequencing showed that the P450 genes CYP71Z18, CYP71C4, CYP71C1, CYP81Q32, and CYP76B6 and the GST genes GSTF11, GSTF1, and GSTU6 were upregulated in at least one resistant population, which indicated their putative roles in cyhalofop-butyl resistance of L. chinensis. Correlation analyses revealed that the constitutive or inducible expression patterns of CYP71C4, CYP71C1, GSTF1, and GSTU6 in L. chinensis were strongly associated with the resistant phenotype. For this reason, attention should be directed towards these genes to elucidate metabolic resistance to cyhalofop-butyl in L. chinensis. The findings of this study improve the understanding of mechanisms responsible for resistance to ACCase-inhibiting herbicides in grass-weed species at the molecular level, thus aiding in the development of weed management strategies that delay the emergence of resistance to this class of pest control products.
Enhancing English as a foreign language university students’ writing through explicit instruction of conjunctions as cohesive devices: An experimental study
Authors: Alawerdy Arwa Saleh , Alalwi Fahd Shehail
Published: November 2022

Cohesion has long been regarded as one of the main components of successful writing. The basic assumption of this concept is that the interpretation of one element in a text is based on the interpretation of another in the same text. It has been noticed that Saudi EFL students seem to lack some basics in writing a cohesive paragraph. Certain conjunctions were overused, underused, or misused. The present empirical study, therefore, attempted to investigate the impact of explicit teaching of conjunctions as cohesive devices on the development of paragraph writing. The subjects of this study were 43 EFL students at a Saudi University studying in the first year. Data were collected from pre and post tests and analyzed qualitatively and quantitatively. Results showed significant differences between the experimental and control groups in the post test. Pedagogical implications are presented.
Peer effects of working capital management: Considering the moderating effect of knowledge flow
Authors: Zhao Mingyuan , Ming Bingxin , Li Yingqing , Shi Junran
Published: November 2022

An important form of human learning and cognition is imitation. In environments where uncertainty is more incremental, imitation of peers is a natural response to uncertainty. While there are substantial literature documenting peer effects in other settings, the study of peer effects in working capital management is novel; little research exists on peer effects in working capital management and their impact mechanism. Using data of China’s listed firms from 2010 to 2021, we empirically demonstrate significant peer effects due to working capital management. Firstly, we find that the behavior of working capital management of firms in the same industry is positively related to a firm’s working capital management. We used peer firms’ target debt ratio as an instrumental variable to address potential endogeneity problem. Secondly, the moderating effects test shows that the positive relationship between the behavior of working capital management of firms in the same industry and a firm’s working capital management behavior is moderated by knowledge flow. Meanwhile, the peer effects in the high group of knowledge flow are greater than that of in the low group of knowledge flow. The study is based on the Active Intermodal Matching theory of psychology. It enriches the research findings on the moderating effect of peer effects and has important implications for policymaking to stimulate the economy.
Adjustment in third culture kids: A systematic review of literature
Authors: Jones Emma Marchal , Reed Marnie , Gaab Jens , Ooi Yoon Phaik
Published: November 2022

Third Culture Kids (TCKs) are children of expatriates who live in a culture other than their country of nationality or their parent's country of nationality for a significant part of their childhood. Past research has indicated that adjustment is a key factor in the success of global mobility. However, current research in the area of TCK adjustment is lacking. This systematic review aims to present and summarize all available published scientific data on the adjustment of internationally mobile children and adolescents who relocate with their families. We aim to understand factors related to TCK adjustment, highlight lacking research areas, and define areas of interest for future research. The eligibility criteria for inclusion in the review were: traditional TCKs; aged 7–17 years; measures taken during the relocation; outcome variables of wellbeing, psychological adjustment or social adjustment, or socio-cultural adjustment or adjustment. An initial search across eight databases in December 2021 yielded 9,433 studies, which were included in COVIDENCE and reviewed independently by two researchers at each phase. We finally included 14 studies in this study, 10 of which presented quantitative data. Extracted quantitative and qualitative studies were abstracted, and the main findings are presented using a consistent grid of codes: an initial computerized lexical scan (Leximancer) of all included papers generated a preliminary list of topics and their frequencies. We refined these initial topics using the most prominent theories around the topics of TCK, adjustment, and the extracted theories from selected papers and created a codebook. Then we abstracted the quantitative data from the selected studies and organized the statistically significant findings according to the codes. Lastly, we abstracted and synthesized the findings from qualitative studies. Efforts were made to present the available data within a reading grid, which enhances the understanding of mechanisms specific to the sample population and also makes it apparent where more research is needed. Specifically, findings suggest a need for a more inclusive multi-trajectory adjustment model and a better definition of the ecological sample. The coding system for the extraction and analysis in this systematic review may be a guide for researchers planning future studies on TCK adjustment.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020151071, identifier: CRD42020151071.
Evaluation and comparison of the sensitivity of three commercial RT-qPCR kits used for the detection of SARS-CoV-2 in Santiago, Chile
Authors: Luraschi Roberto , Santibáñez Álvaro , Barrera-Avalos Carlos , Vallejos-Vidal Eva , Mateluna-Flores Carlos , Alarcón Javiera , Cayunao Javiera , Mella-Torres Andrea , Hernández Felipe , Inostroza-Molina Ailen , Valdés Daniel , Imarai Mónica , Acuña-Castillo Claudio , Reyes-López Felipe E. , Sandino Ana María
Published: November 2022

Introduction

The COVID-19 pandemic is still in force, causing global public health challenges and threats. Although vaccination and herd immunity have proven to be the most efficient way to control the pandemic, massive and early testing of patients using the RT-qPCR technique is crucial for constant genomic surveillance. The appearance of variants of SARS-CoV-2 with new mutations can reduce the efficiency of diagnostic detection. In this sense, several commercial RT-qPCR kits have been the target of extensive analysis because low assay performance could lead to false-negative diagnoses.

Methods

In this study, we evaluated the performance of three commercial RT-qPCR kits; Thermo Fisher (TaqMan 2019-nCoV Assay Kit v1), BGI and Roche (LightCycler® Multiplex RNA Virus Master) used for the diagnosis of COVID-19 throughout the pandemic in Santiago de Chile.

Results

Under our best assay conditions, we found significant differences in Cq amplification values for control and viral probes, against the same nasopharyngeal swab samples (NPSs). In addition, in some cases, the sensitivity of the RT-qPCR kits decreased against viral variants.

Conclusion

Our study suggests evaluating the RT-qPCR kits used to detect SARS-CoV-2 because variants such as Omicron, which has several mutations, can compromise their detection and underestimate viral circulation.
Spatiotemporal impact of non-pharmaceutical interventions against COVID-19 on the incidence of infectious diarrhea in Xi'an, China
Authors: Zhang Hui , Shen Li , Sun Minghao , Zhao Chenxi , Li Qian , Yang Zurong , Liu Jifeng , Liu Kun , Xiao Bo
Published: November 2022

Background

Non-pharmaceutical interventions (NPIs) against COVID-19 may prevent the spread of other infectious diseases. Our purpose was to assess the effects of NPIs against COVID-19 on infectious diarrhea in Xi'an, China.

Methods

Based on the surveillance data of infectious diarrhea, and the different periods of emergence responses for COVID-19 in Xi'an from 2011 to 2021, we applied Bayesian structural time series model and interrupted time series model to evaluate the effects of NPIs against COVID-19 on the epidemiological characteristics and the causative pathogens of infectious diarrhea.

Findings

A total of 102,051 cases of infectious diarrhea were reported in Xi'an from 2011 to 2021. The Bayesian structural time series model results demonstrated that the cases of infectious diarrhea during the emergency response period was 40.38% lower than predicted, corresponding to 3,211 fewer cases, during the COVID-19 epidemic period of 2020–2021. The reduction exhibited significant variations in the demography, temporal and geographical distribution. The decline in incidence was especially evident in children under 5-years-old, with decreases of 34.09% in 2020 and 33.99% in 2021, relative to the 2017–2019 average. Meanwhile, the incidence decreased more significantly in industrial areas.

Interpretation

NPIs against COVID-19 were associated with short- and long-term reductions in the incidence of infectious diarrhea, and this effect exhibited significant variations in epidemiological characteristics.
Proportion and trend in the age of cigarette smoking initiation among adolescent smoking experiencers aged 13–15 years in 148 countries/territories
Authors: Xing Shuhui , Zhao Min , Magnussen Costan G. , Xi Bo
Published: November 2022

Background

Limited studies have assessed the recent proportion and trend in the age of cigarette smoking initiation among adolescent smoking experiencers globally. We aimed to assess the recent global proportion, associated factors and the long-term trend of the initiated age of cigarette smoking among adolescent smoking experiencers.

Methods

We used data from the most recent Global Youth Tobacco Survey on 99,728 adolescent smoking experiencers aged 13–15 years from 144 countries/territories (hereafter “countries”) that had conducted at least one survey in 2010–20, to assess the recent proportion of the age of cigarette smoking initiation. Additionally, we used data from 148 countries that had conducted ≥2 surveys between 1999 and 2020, to assess the trend in the average age of cigarette smoking initiation.

Results

Among 99,728 adolescent smoking experiencers aged 13–15 years, the proportions of initiating cigarette smoking when aged ≤ 9 years, 10–11 years, 12–13 years, and 14–15 years were 22.8% (95%CI 21.3–24.4), 18.8% (17.3–20.2), 36.8% (34.5–39.2), and 21.6% (19.9–23.2), respectively. The average age of cigarette smoking initiation decreased by 0.44 years per 5 calendar-years averagely in 17 (11.5%) of 148 countries, was unchanged in 95 (64.2%) countries, and increased by 0.38 years per 5 calendar-years averagely in 36 (24.3%) countries. Higher income category (reference group: low-income countries; lower-middle-income: OR = 0.44, 95%CI = 0.28–0.70; upper-middle-income: OR = 0.56, 95%CI = 0.38–0.83; high-income: OR = 0.35, 95%CI = 0.22–0.53) and Framework Convention on Tobacco Control ratification (OR = 0.51, 95%CI = 0.42–0.63) were inversely associated with early cigarette smoking initiation.

Conclusions

A substantial proportion (~80%) of adolescent smoking experiencers aged 13–15 years initiated cigarette smoking before 13 years, and the average age of cigarette smoking initiation decreased or remained unchanged in nearly three quarters of the countries surveyed. These findings emphasize that national governments around the world need to strengthen intervention strategies and measures aimed at children and adolescents to prevent smoking up-take.
Different components of air pollutants and neurological disorders
Authors: Fu Chunlia , Kuang Daibing , Zhang He , Ren Jinxin , Chen Jialong
Published: November 2022

The harmful effects of air pollution can cause various diseases. Most research on the hazards of air pollution focuses on lung and cardiovascular diseases. In contrast, the impact of air pollution on neurological disorders is not widely recognized. Air pollution can cause various neurological conditions and diseases, such as neural inflammation, neurodegeneration, and cerebrovascular barrier disorder; however, the mechanisms underlying the neurological diseases induced by various components of air pollutants remain unclear. The present paper summarizes the effects of different components of air pollutants, including particulate matter, ozone, sulfur oxides, carbon oxides, nitrogen oxides, and heavy metals, on the nervous system and describes the impact of various air pollutants on neurological disorders, providing ideas for follow-up research.
Factors determining the quality of health services provided to COVID-19 patients from the perspective of healthcare providers: Based on the Donabedian model
Authors: Moayed Malihe Sadat , Khalili Robabe , Ebadi Abbas , Parandeh Akram
Published: November 2022

Objectives

The present study aims to explain factors determining the quality of health services provided to COVID-19 patients from the perspective of healthcare providers based on the Donabedian model.

Method

This qualitative study was conducted at a referral hospital on COVID-19 patients in Tehran, in 2020. The data were collected through individual and semi-structured interviews from 20 participants using the purposive sampling method. Besides, data analysis was conducted simultaneously using the directed content analysis method.

Results

Data analysis results produced 850 primary codes in three predetermined categories of the Donabedian model, including the structure (organizational readiness and continuous training), the process (effective management and leadership, safe care, and comprehensive care measures) and outcomes (professional excellence, quantitative and qualitative improvements in hospital services, and acceptability of healthcare professionals).

Conclusion

The results of this study can help managers better understand how a public health crisis affects the structure of organizations providing care and treatment, quality of treatment processes in the organization, and the consequences. In addition, this study can be used as a model for optimizing the structures and processes to improve outcomes.
Comparing Different Resonant Control Approaches for Torque Ripple Minimisation in Electric Machines
Authors: Steffen Thomas , Rafaq Muhammad Saad , Midgley Will
Published: November 2022

Electric machines are highly efficient and highly controllable actuators, but they do still suffer from a number of imperfections. One of them is torque ripple, which introduces high frequency harmonics into the motion. One (cost- and performance-neutral) countermeasure is to apply control that counters the torque ripple. This paper compares several single-input single-output (SISO) control approaches for feedback control of torque ripple of a Permanent Magnet Synchronous Machine (PMSM). The baseline is PI (proportional-integral) control, which does not suppress torque ripple, and the most popular control approach is proportional-integral resonant (PIR) control. Both are compared to an advanced PIR controller (PIRA), frequency modulation, a mixed sensitivity design, and an iterative learning controller (ILC). The analysis demonstrates that PIR control, mixed sensitivity state feedback, and the modulating controller achieve identical behaviour. The choice between these three options is therefore dependent on preferences for the design methodology, or on implementation factors. The PIRA and the ILC on the other hand show more sophisticated behaviour that may be advantageous for certain applications, at the expense of higher complexity.
Ammonia and Phosphine in the Clouds of Venus as Potentially Biological Anomalies
Authors: Cleland Carol E. , Rimmer Paul B.
Published: November 2022

We are of the opinion that several anomalies in the atmosphere of Venus provide evidence of yet-unknown processes and systems that are out of equilibrium. The investigation of these anomalies on Venus should be open to a wide range of explanations, including unknown biological activity. We provide an overview of two anomalies, the tentative detection of ammonia and phosphine in Venus’s atmosphere. These anomalies fly in the face of the tacit assumption that the atmosphere of Venus must be in chemical redox equilibrium, an assumption connected to the belief that Venus is lifeless. We then discuss several major past discoveries in astronomy, biology and geology, which lead to the abandonment of certain assumptions held by many scientists as though they were well-established principles. The anomalies of ammonia and phosphine in the atmosphere of Venus are placed in the context of these historical discoveries. This context supports our opinion that persistence by the community in the exploration of these anomalies with a skeptical eye towards tacit assumptions will increase the chances of making profound discoveries about the atmosphere of Venus and the diverse and often strange nature of planetary environments. To be submitted to Aerospace Special Issue “The Search for Signs of Life on Venus: Science Objectives and Mission Designs”.
Playful Probing: Towards Understanding the Interaction with Machine Learning in the Design of Maintenance Planning Tools
Authors: Ribeiro Jorge , Roque Licínio
Published: November 2022

In the context of understanding interaction with artificial intelligence algorithms in a decision support system, this study addresses the use of a playful probe as a potential speculative design approach. We describe the process of researching a new machine learning (ML)-based planning tool for maintenance based on aircraft conditions and the challenge of investigating how playful probes can enable end-user participation during the process of design. Using a design science research approach, we designed a playful probe protocol and materials and evaluated results by running a participatory design workshop. With this approach, participants facilitated speculative design insights into understandable interactions, especially with ML interaction. The article contributes with a design of a playful probe exercise to collaboratively study the adjustment of practices for CBM and a set of concrete insights on understandable interactions with CBM.
Auto Sweptback Wing Based on Low Scattering Demand for an Unmanned Aerial Vehicle in Phase Flight
Authors: Zhou Zeyang , Huang Jun
Published: November 2022

In order to study the optimal sweepback angle when a variant unmanned aerial vehicle (UAV) exhibits a low radar cross-section (RCS) indicator during phase flight, an auto sweep scheme based on electromagnetic scattering evaluation and an improved particle swarm optimization algorithm was presented in this article. An aircraft model with variable swept wings was built, and high-precision grids were used to discretize the target surface. The results showed that the optimal sweep angle did not change with the increase in the initial azimuth angle when the observation field was horizontal and the ending azimuth was 90°. While the increase in the elevation angle affected the optimal sweepback angle of the aircraft under the given conditions, when the observation initial azimuth angle was 90°, the auto sweep scheme could reduce the mean and some minima of the RCS indicator curve of the aircraft and could provide the aircraft with an optimal sweep angle under different observation conditions. The presented method was effective in learning the optimal sweep angle of the aircraft when low scattering characteristics were required during the phase flight.
Variation of Soil Nitrogen, Organic Carbon, and Waxy Wheat Yield Using Liquid Organic and Mineral Fertilizers
Authors: Petraityte Danute , Ceseviciene Jurgita , Arlauskiene Ausra , Slepetiene Alvyra , Skersiene Aida , Gecaite Viktorija
Published: November 2022

Biogas slurry is widely used to fertilize crops. However, their impact on soil parameters and waxy winter wheat (Triticum aestivum L.) nutrition is poorly understood. The aim of this research was to determine the influence of liquid anaerobic digestate and pig slurry applied to waxy winter wheat on the dynamics of soil organic carbon (SOC) and total nitrogen (Ntot) in different forms on grain yield, and to compare them with the use of ammonium nitrate. The nitrogen rates (kg N·ha−1) used for fertilization were N0, N60, N120, and N120+50. The study showed that the variation of nitrate nitrogen (N-NO3) and water-extractable organic carbon (WEOC) in the soil during the growing season depended on N fertilizer rates, meteorological conditions of the year, and, to a lesser extent, on fertilizer forms. Meteorological conditions were responsible for the demand and supply of nutrients from the soil by the waxy winter wheat variety. This determined the wheat yield and the variation in the soil parameters studied. Over the 2 years, the soil C:N ratio decreased, especially at the medium and high N fertilizer rates. The lowest changes were observed in the unfertilized and fertilized plots at a rate of 60 kg N·ha−1.
Scion–Rootstock Relationship: Molecular Mechanism and Quality Fruit Production
Authors: Shivran Mukesh , Sharma Nimisha , Dubey Anil Kumar , Singh Sanjay Kumar , Sharma Neha , Sharma Radha Mohan , Singh Narendra , Singh Rakesh
Published: November 2022

Most tree fruits are commercially grown on different root systems, hence called composite plants. The section provides the root system as the rootstock, and the atop ground portion is called the scion. The combination is selected based on different traits of scion varieties, rootstock, and prevailing edaphic situations. The dated back plant propagation technique of joining two plants (grafting/budding) that directly communicates new physiological traits to the desirable scion variety from the rootstock remains unclear. In spite of this, this propagation technique continues widely applied in the multiplication of several fruit plant species. In a grafted plant, rootstocks impacted the scion variety’s growth, yield and quality attributes, physiology, nutrient accumulation as well as biotic and abiotic stress tolerance in many ways. Modern research in plant science for next-generation sequencing providing new vital information about the molecular interactions in composite plants multiplied using grafting. Now it was confirmed that genetic exchange is occurring between rootstock and scion variety through grafting joints. In this aspect, we discuss the process and the molecular mechanism of rootstock scion interactions. This review finally explains the dynamics of rootstock–scion interactions as well as their effect on physiology in terms of production, environmental stresses, and fruit quality. The morphological, physiochemical, and molecular mechanisms have been reviewed to develop an integrated understanding of this unknowable process that questions existing genetic paradigms. The present review summarizes the reported molecular mechanism between scion and rootstock and the impact of rootstocks on the production biology of scion varieties of economically important fruit crops and identifies numerous key points to consider when conducting rootstock scion interaction experiments. Rootstocks may offer a non-transgenic approach to rapidly respond to the changing environment and expand agricultural production of perennial fruit crops where grafting is possible in order to meet the global demand for fruit, food, and demands of the future.
Genome-Wide Identification and Expression Analysis of BrATGs and Their Different Roles in Response to Abiotic Stresses in Chinese Cabbage
Authors: Hu Yuanfeng , Zhang Ming , Yin Fengrui , Cao Xiaoqun , Fan Shuying , Wu Caijun , Xiao Xufeng
Published: November 2022

Autophagy is an important degradation pathway that maintains cellular homeostasis in living organisms and plays a key role in plant fitness and immunity. To date, more than 30 autophagy-related genes (ATGs) have been identified in model plants such as Arabidopsis. However, autophagy in Chinese cabbage, the largest cultivated vegetable crop in China, has scarcely been studied. We identified 64 Chinese cabbage autophagy-associated genes, named BrATGs, at the genome-wide level. The majority of the BrATGs were highly conserved over a long evolutionary period, and the expression patterns indicated that BrATGs were most highly expressed in the healing tissues and flowers. Furthermore, BrATGs responded to the stresses of the heavy metal Cd, drought, salt, and low and high temperatures to varying degrees. Among them, BrATG8c/8j was specifically induced in response to drastic temperature changes; BrATG4c was upregulated only in response to drought and salt stress; and BrATG8f/10/VTI12C was highly expressed only in response to Cd stress. This work will advance the understanding of the molecular mechanisms underlying the abiotic stress response in Chinese cabbage.