bluuebunny/crossref_metadata_2025 huggingface dataset:
Short-Term Associations of Fine Particulate Matter and Synoptic Weather Types with Cardiovascular Mortality: An Ecological Time-Series Study in Shanghai, China
Authors: Tian Qing , Li Mei , Montgomery Scott , Fang Bo , Wang Chunfang , Xia Tian , Cao Yang
Published: February 2020

Background: Exposures to both ambient fine particulate matter (PM2.5) and extreme weather conditions have been associated with cardiovascular disease (CVD) deaths in numerous epidemiologic studies. However, evidence on the associations with CVD deaths for interaction effects between PM2.5 and weather conditions is still limited. This study aimed to investigate associations of exposures to PM2.5 and weather conditions with cardiovascular mortality, and further to investigate the synergistic or antagonistic effects of ambient air pollutants and synoptic weather types (SWTs). Methods: Information on daily CVD deaths, air pollution, and meteorological conditions between 1 January 2012 and 31 December 2014 was obtained in Shanghai, China. Generalized additive models were used to assess the associations of daily PM2.5 concentrations and meteorological factors with CVD deaths. A 15-day lag analysis was conducted using a polynomial distributed lag model to access the lag patterns for associations with PM2.5. Results: During the study period, the total number of CVD deaths in Shanghai was 59,486, with a daily mean of 54.3 deaths. The average daily PM2.5 concentration was 55.0 µg/m3. Each 10 µg/m3 increase in PM2.5 concentration was associated with a 1.26% (95% confidence interval (CI): 0.40%, 2.12%) increase in CVD mortality. No SWT was statistically significantly associated with CVD deaths. For the interaction between PM2.5 and SWT, statistically significant interactions were found between PM2.5 and cold weather, with risk for PM2.5 in cold dry SWT decreasing by 1.47% (95% CI: 0.54%, 2.39%), and in cold humid SWT the risk decreased by 1.45% (95% CI: 0.52%, 2.36%). In the lag effect analysis, statistically significant positive associations were found for PM2.5 in the 1–3 lag days, while no statistically significant effects were found for other lag day periods. Conclusions: Exposure to PM2.5 was associated with short-term increased risk of cardiovascular deaths with some lag effects, while the cold weather may have an antagonistic effect with PM2.5. However, the ecological study design limited the possibility to identify a causal relationship, so prospective studies with individual level data are warranted.
Examining Users’ Adoption of Precision Medicine: The Moderating Role of Medical Technical Knowledge
Authors: Wang Xingyuan , Liu Yun , Liu Hongchen
Published: February 2020

Precision medical technologies have received a great deal of attention, but promoting such technologies remains a problem for enterprises and medical institutions. Adopting the unified theory of acceptance and use of technology (UTAUT) model and the health belief model (HBM), this study investigated the key factors affecting users’ willingness to adopt precision medicine (PM) in terms of technical factors and external stimuli. Based on 415 questionnaires, performance expectancy, price value, social influence, and perceived threat of disease were found to significantly increase users willingness to adopt PM; meanwhile, privacy risks had the opposite effect. Knowledge about PM was found to strengthen the positive effect of performance expectancy, price value, social influence, and perceived threat of disease on willingness to adopt PM and weaken the negative effect of privacy risk. This study demonstrates the successful application of UTAUT to the medical field while also providing guidance for the promotion of PM.
Seven Weeks of Jump Training with Superimposed Whole-Body Electromyostimulation Does Not Affect the Physiological and Cellular Parameters of Endurance Performance in Amateur Soccer Players
Authors: Wirtz Nicolas , Filipovic André , Gehlert Sebastian , de Marées Markus , Schiffer Thorsten , Bloch Wilhelm , Donath Lars
Published: February 2020

Intramuscular density of monocarboxylate-transporter (MCT) could affect the ability to perform high amounts of fast and explosive actions during a soccer game. MCTs have been proven to be essential for lactate shuttling and pH regulation during exercise and can undergo notable adaptational changes depending on training. The aim of this study was to evaluate the occurrence and direction of potential effects of a 7-weeks training period of jumps with superimposed whole-body electromyostimulation on soccer relevant performance surrogates and MCT density in soccer players. For this purpose, 30 amateur soccer players were randomly assigned to three groups. One group performed dynamic whole-body strength training including 3 x 10 squat jumps with WB-EMS (EG, n = 10) twice a week in addition to their daily soccer training routine. A jump training group (TG, n = 10) performed the same training routine without EMS, whereas a control group (CG, n = 8) merely performed their daily soccer routine. 2 (Time: pre vs. post) x 3 (group: EG, TG, CG) repeated measures analyses of variance (rANOVA) revealed neither a significant time, group nor interaction effect for VO2peak, Total Time to Exhaustion and Lamax as well as MCT-1 density. Due to a lack of task-specificity of the underlying training stimuli, we conclude that seven weeks of WB-EMS superimposed to jump exercise twice a week does not relevantly influence aerobic performance or MCT density.
Active and Fit Communities. Associations between Neighborhood Walkability and Health-Related Fitness in Adults
Authors: McCormack Gavin R. , Frehlich Levi , Blackstaffe Anita , Turin Tanvir C. , Doyle-Baker Patricia K.
Published: February 2020

There are many health benefits of regular physical activity and improving physical fitness levels can reduce the risk of chronic disease. Accumulating evidence suggests the neighborhood built environment is important for supporting physical activity; however, few studies have investigated the contribution of the neighborhood built environment to fitness levels. We examined the associations between objectively-determined and self-reported neighborhood walkability and overall and specific components of perceived health-related fitness (cardiorespiratory, muscular strength, and flexibility) in a random sample of 592 adults from two areas of Calgary (Canada). Participants provided complete data to an online questionnaire capturing perceived cardiorespiratory fitness (CRF), muscular strength (MST), flexibility, moderate-to-vigorous intensity physical activity (MVPA), resistance training, and sociodemographic characteristics. The questionnaire also captured participant’s perceptions of their neighborhood’s walkability (Physical Activity Neighborhood Environment Scale; PANES) and the physical activity supportiveness of neighborhood parks (Park Perceptions Index; PPI). Objectively-measured neighborhood walkability was estimated using Walk Score®. The average (SD) age of participants was 46.6 (14.8) years and 67.2% were female. Participants, on average, participated in at least 30-minutes of MVPA on 3.4 (2.1) days/week and undertook resistance training 2.0 (1.8) days/week. Adjusting for covariates, Walk Score® was not associated with any fitness outcomes. Adjusting for covariates, the PANES index was positively associated (p < 0.05) with CRF, MST, flexibility, and overall fitness and the PPI was positively associated (p < 0.05) with all fitness outcomes except MST. Our findings provide novel preliminary evidence suggesting the neighborhood built environment may be important for supporting higher health-related fitness levels in adults.
Older Adults’ Perceptions of the Usefulness of Technologies for Engaging in Physical Activity: Using Focus Groups to Explore Physical Literacy
Authors: Campelo Alexandre Monte , Katz Larry
Published: February 2020

Insufficient physical activity (PA) levels observed among older adults remain extremely high and pose a danger to developing and maintaining their physical literacy (PL). Each person’s level of PL partly depends on their physical and cognitive skills, confidence level, and degree of motivation to practice PA daily. New technologies, such as exergames and wearable fitness trackers, may enable older adults to increase their PL, stimulating uptake and ongoing PA participation. Objective: This focus group study aims to describe older adults’ perceptions of the use of technologies to engage in physical exercise programs. Methods: Fifteen participants were randomly selected from a sample of 40 older adults who completed a randomized controlled trial that investigated the benefits of using technology in the context of group-based exercise programs. Separate post-intervention focus groups were performed with an exergaming group, a conventional physical training group, and a no training group (control). Data were mapped onto constructs from the four domains of PL: affective, physical, cognitive, and behavioral. Results: Generally, participants expressed positive perceptions about the benefits of using technology to engage in PA. These positive feelings outweighed the costs and the lack of familiarization with technology. Common themes for the three groups emerged from the discussions and included familiarization with technology, using fitness tracker to monitor PA, previous exposure to technology, and interaction with peers, staff members, and relatives. In particular, participants from the exergaming group explored the ideas of training their cognitive skills while using the exergame accessories, exercising in an alternative way, competitive versus cooperative play, changes in sense of humor, skill transferability from game to real environment, progressions of the exercise intensities, and the potential use of exergames for rehabilitation. Conclusions: Participants in this study reported positive perceptions about implementing technology into exercise. Emphasizing the benefits of using technology in group-based exercise programs may increase older adults’ PL levels and their future technology adoption. The potential implementation of technology into conventional exercise programs should focus on older adults’ lifelong values, biopsychosocial conditions, and the possibility of reducing age-related risk of injuries and chronic diseases.
Bone Control of Muscle Function
Authors: Battafarano Giulia , Rossi Michela , Marampon Francesco , Minisola Salvatore , Del Fattore Andrea
Published: February 2020

Bone and muscle represent a single functional system and are tightly connected to each other. Indeed, diseases characterized by alterations of muscle physiology have effects on bone remodeling and structure and vice versa. Muscle influence on bone has been deeply studied, and recent studies identified irisin as new molecule involved in this crosstalk. Muscle regulation by bone needs to be extensively investigated since in the last few years osteocalcin was recognized as a key molecule in the bone–muscle interaction. Osteocalcin can exist in two forms with different degrees of carboxylation. The undercarboxylated form of osteocalcin is a hormone released by the bone matrix during the osteoclast bone resorption and can bind its G-protein coupled receptor GPRC6A expressed in the muscle, thus regulating its function. Recently, this hormone was described as an antiaging molecule for its ability to regulate bone, muscle and cognitive functions. Indeed, the features of this bone-related hormone were used to test a new therapeutic approach for sarcopenia, since injection of osteocalcin in older mice induces the acquirement of physical abilities of younger animals. Even if this approach should be tested in humans, osteocalcin represents the most surprising molecule in endocrine regulation by the skeleton.
Urinary NMR Profiling in Pediatric Acute Kidney Injury—A Pilot Study
Authors: Muhle-Goll Claudia , Eisenmann Philipp , Luy Burkhard , Kölker Stefan , Tönshoff Burkhard , Fichtner Alexander , Westhoff Jens H.
Published: February 2020

Acute kidney injury (AKI) in critically ill children and adults is associated with significant short- and long-term morbidity and mortality. As serum creatinine- and urine output-based definitions of AKI have relevant limitations, there is a persistent need for better diagnostics of AKI. Nuclear magnetic resonance (NMR) spectroscopy allows for analysis of metabolic profiles without extensive sample manipulations. In the study reported here, we examined the diagnostic accuracy of NMR urine metabolite patterns for the diagnosis of neonatal and pediatric AKI according to the Kidney Disease: Improving Global Outcomes (KDIGO) definition. A cohort of 65 neonatal and pediatric patients (0–18 years) with established AKI of heterogeneous etiology was compared to both a group of apparently healthy children (n = 53) and a group of critically ill children without AKI (n = 31). Multivariate analysis identified a panel of four metabolites that allowed diagnosis of AKI with an area under the receiver operating characteristics curve (AUC-ROC) of 0.95 (95% confidence interval 0.86–1.00). Especially urinary citrate levels were significantly reduced whereas leucine and valine levels were elevated. Metabolomic differentiation of AKI causes appeared promising but these results need to be validated in larger studies. In conclusion, this study shows that NMR spectroscopy yields high diagnostic accuracy for AKI in pediatric patients.
The Role of Brassinosteroids in Controlling Plant Height in Poaceae: A Genetic Perspective
Authors: Castorina Giulia , Consonni Gabriella
Published: February 2020

The most consistent phenotype of the brassinosteroid (BR)-related mutants is the dwarf habit. This observation has been reported in every species in which BR action has been studied through a mutational approach. On this basis, a significant role has been attributed to BRs in promoting plant growth. In this review, we summarize the work conducted in rice, maize, and barley for the genetic dissection of the pathway and the functional analysis of the genes involved. Similarities and differences detected in these species for the BR role in plant development are presented. BR promotes plant cell elongation through a complex signalling cascade that modulates the activities of growth-related genes and through the interaction with gibberellins (GAs), another class of important growth-promoting hormones. Evidence of BR–GA cross-talk in controlling plant height has been collected, and mechanisms of interaction have been studied in detail in Arabidopsis thaliana and in rice (Oryza sativa). The complex picture emerging from the studies has highlighted points of interaction involving both metabolic and signalling pathways. Variations in plant stature influence plant performance in terms of stability and yield. The comprehension of BR’s functional mechanisms will therefore be fundamental for future applications in plant-breeding programs.
Molecular Mechanism of Epidermal Barrier Dysfunction as Primary Abnormalities
Authors: Lee Ai-Young
Published: February 2020

Epidermal barrier integrity could be influenced by various factors involved in epidermal cell differentiation and proliferation, cell–cell adhesion, and skin lipids. Dysfunction of this barrier can cause skin disorders, including eczema. Inversely, eczema can also damage the epidermal barrier. These interactions through vicious cycles make the mechanism complicated in connection with other mechanisms, particularly immunologic responses. In this article, the molecular mechanisms concerning epidermal barrier abnormalities are reviewed in terms of the following categories: epidermal calcium gradients, filaggrin, cornified envelopes, desquamation, and skin lipids. Mechanisms linked to ichthyoses, atopic dermatitis without exacerbation or lesion, and early time of experimental irritation were included. On the other hand, the mechanism associated with epidermal barrier abnormalities resulting from preceding skin disorders was excluded. The molecular mechanism involved in epidermal barrier dysfunction has been mostly episodic. Some mechanisms have been identified in cultured cells or animal models. Nonetheless, research into the relationship between the causative molecules has been gradually increasing. Further evidence-based systematic data of target molecules and their interactions would probably be helpful for a better understanding of the molecular mechanism underlying the dysfunction of the epidermal barrier.
Metabolomics to Improve the Diagnostic Efficiency of Inborn Errors of Metabolism
Authors: Mordaunt Dylan , Cox David , Fuller Maria
Published: February 2020

Early diagnosis of inborn errors of metabolism (IEM)—a large group of congenital disorders—is critical, given that many respond well to targeted therapy. Newborn screening programs successfully capture a proportion of patients enabling early recognition and prompt initiation of therapy. For others, the heterogeneity in clinical presentation often confuses diagnosis with more common conditions. In the absence of family history and following clinical suspicion, the laboratory diagnosis typically begins with broad screening tests to circumscribe specialised metabolite and/or enzyme assays to identify the specific IEM. Confirmation of the biochemical diagnosis is usually achieved by identifying pathogenic genetic variants that will also enable cascade testing for family members. Unsurprisingly, this diagnostic trajectory is too often a protracted and lengthy process resulting in delays in diagnosis and, importantly, therapeutic intervention for these rare conditions is also postponed. Implementation of mass spectrometry technologies coupled with the expanding field of metabolomics is changing the landscape of diagnosing IEM as numerous metabolites, as well as enzymes, can now be measured collectively on a single mass spectrometry-based platform. As the biochemical consequences of impaired metabolism continue to be elucidated, the measurement of secondary metabolites common across groups of IEM will facilitate algorithms to further increase the efficiency of diagnosis.
Assessment of Nuclear and Mitochondrial DNA, Expression of Mitochondria-Related Genes in Different Brain Regions in Rats after Whole-Body X-ray Irradiation
Authors: Abdullaev Serazhutdin , Gubina Nina , Bulanova Tatiana , Gaziev Azhub
Published: February 2020

Studies of molecular changes occurred in various brain regions after whole-body irradiation showed a significant increase in terms of the importance in gaining insight into how to slow down or prevent the development of long-term side effects such as carcinogenesis, cognitive impairment and other pathologies. We have analyzed nDNA damage and repair, changes in mitochondrial DNA (mtDNA) copy number and in the level of mtDNA heteroplasmy, and also examined changes in the expression of genes involved in the regulation of mitochondrial biogenesis and dynamics in three areas of the rat brain (hippocampus, cortex and cerebellum) after whole-body X-ray irradiation. Long amplicon quantitative polymerase chain reaction (LA-QPCR) was used to detect nDNA and mtDNA damage. The level of mtDNA heteroplasmy was estimated using Surveyor nuclease technology. The mtDNA copy numbers and expression levels of a number of genes were determined by real-time PCR. The results showed that the repair of nDNA damage in the rat brain regions occurs slowly within 24 h; in the hippocampus, this process runs much slower. The number of mtDNA copies in three regions of the rat brain increases with a simultaneous increase in mtDNA heteroplasmy. However, in the hippocampus, the copy number of mutant mtDNAs increases significantly by the time point of 24 h after radiation exposure. Our analysis shows that in the brain regions of irradiated rats, there is a decrease in the expression of genes (ND2, CytB, ATP5O) involved in ATP synthesis, although by the same time point after irradiation, an increase in transcripts of genes regulating mitochondrial biogenesis is observed. On the other hand, analysis of genes that control the dynamics of mitochondria (Mfn1, Fis1) revealed that sharp decrease in gene expression level occurred, only in the hippocampus. Consequently, the structural and functional characteristics of the hippocampus of rats exposed to whole-body radiation can be different, most significantly from those of the other brain regions.
Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis
Authors: Han Deguo , Du Man , Zhou Zhengyi , Wang Shuang , Li Tiemei , Han Jiaxin , Xu Tianlong , Yang Guohui
Published: February 2020

NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 °C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS).
Early Programming of Adult Systemic Essential Hypertension
Authors: Guarner-Lans Verónica , Ramírez-Higuera Abril , Rubio-Ruiz María Esther , Castrejón-Téllez Vicente , Soto María Elena , Pérez-Torres Israel
Published: February 2020

Cardiovascular diseases are being included in the study of developmental origins of health and disease (DOHaD) and essential systemic hypertension has also been added to this field. Epigenetic modifications are one of the main mechanisms leading to early programming of disease. Different environmental factors occurring during critical windows in the early stages of life may leave epigenetic cues, which may be involved in the programming of hypertension when individuals reach adulthood. Such environmental factors include pre-term birth, low weight at birth, altered programming of different organs such as the blood vessels and the kidney, and living in disadvantageous conditions in the programming of hypertension. Mechanisms behind these factors that impact on the programming include undernutrition, oxidative stress, inflammation, emotional stress, and changes in the microbiota. These factors and their underlying causes acting at the vascular level will be discussed in this paper. We also explore the establishment of epigenetic cues that may lead to hypertension at the vascular level such as DNA methylation, histone modifications (methylation and acetylation), and the role of microRNAs in the endothelial cells and blood vessel smooth muscle which participate in hypertension. Since epigenetic changes are reversible, the knowledge of this type of markers could be useful in the field of prevention, diagnosis or epigenetic drugs as a therapeutic approach to hypertension.
Numerical and Experimental Analysis of Material Removal and Surface Defect Mechanism in Scratch Tests of High Volume Fraction SiCp/Al Composites
Authors: Zhao Xu , Gong Yadong , Cai Ming , Han Bing
Published: February 2020

This paper addresses a comprehensive and further insight into the sensitivity of material removal and the surface defect formation mechanism to scratch depth during single-grit scratch tests of 50 vol% SiCp/Al composites. The three-dimensional (3D) finite element model with more realistic 3D micro-structure, particle-matrix interfacial behaviors, particle-particle contact behaviors, particle-matrix contact behaviors and a Johnson-Holmquist-Beissel (JHB) model of SiC was developed. The scratch simulation conducted at scratch velocity 10 mm/min and loading rate 40 N/min revealed that the scratch depth plays a crucial role in material removal and the surface forming process. Brittle fracturing of SiC particles and surface defects become more deteriorative under a large scratch depth ranging from 0.0385 to 0.0764 μm. The above phenomenon can be attributed to the influence of scratch depth on SiC particles’ transport; the increase in the amount of SiC particle transport resulting from an increase of scratch depth raises the occurrence of particle-particle collision which provides hard support and shock for the scratched particles; therefore, brittle fracturing gradually becomes the major removal mode of SiC particles as the scratch depth increases. On the deteriorative surface, various defects are observed; i.e., lateral cracks, interfacial debonding, cavies filled with residually broken particles, etc. The von Mises stress distribution shows that SiC particles bear vast majority of load, and thus present greater stress than the surrounding Al matrix. For example: their ratio of 3 to 30 under the scratch depth of 0.011 mm. Namely, SiC particles impede stress diffusion within the Al matrix. Finally, the SEM images of the scratched surface obtained from the single-grit scratch experiments verify the numerical analysis’s results.
Bioengineering Methods of Analysis and Medical Devices: A Current Trends and State of the Art
Authors: Cicciù Marco
Published: February 2020

Implantology, prosthodontics, and orthodontics in all their variants, are medical and rehabilitative medical fields that have greatly benefited from bioengineering devices of investigation to improve the predictability of clinical rehabilitations. The finite element method involves the simulation of mechanical forces from an environment with infinite elements, to a simulation with finite elements. This editorial aims to point out all the progress made in the field of bioengineering and medicine. Instrumental investigations, such as finite element method (FEM), are an excellent tool that allows the evaluation of anatomical structures and any facilities for rehabilitation before moving on to experimentation on animals, so as to have mechanical characteristics and satisfactory load cycle testing. FEM analysis contributes substantially to the development of new technologies and new materials in the biomedical field. Thanks to the 3D technology and to the reconstructions of both the anatomical structures and eventually the alloplastic structures used in the rehabilitations it is possible to consider all the mechanical characteristics, so that they could be analyzed in detail and improved where necessary.
Effect of Rough Surface Platforms on the Mucosal Attachment and the Marginal Bone Loss of Implants: A Dog Study
Authors: Montero Javier , Fernández-Ruiz Alberto , Pardal-Peláez Beatriz , Jiménez-Guerra Alvaro , Velasco-Ortega Eugenio , Nicolás-Silvente Ana I. , Monsalve-Guil Loreto
Published: February 2020

The preservation of peri-implant tissues is an important factor for implant success. This study aimed to assess the influence of the surface features of a butt-joint platform on soft-tissue attachment and bone resorption after immediate or delayed implant placement. All premolars and first molars of eight Beagle dogs were extracted on one mandible side. Twelve-weeks later, the same surgery was developed on the other side. Five implants with different platform surface configurations were randomly inserted into the post-extracted-sockets. On the healed side, the same five different implants were randomly placed. Implants were inserted 1 mm subcrestal to the buccal bony plate and were connected to abutments. The primary outcome variables were the supracrestal soft tissue (SST) adaptation and the bone resorption related to the implant shoulder. The SST height was significantly larger in immediate implants (IC95% 3.9–4.9 mm) compared to delayed implants (IC95% 3.1–3.5 mm). Marginal bone loss tended to be higher in immediate implants (IC95% 0.4–0.9 mm) than in delayed implants (IC95% 0.3–0.8 mm). Linear-regression analysis suggested that the SST height was significantly affected by the configuration of the platform (0.3–1.9 mm). Roughened surface platforms resulted in higher SST height when compared to machined surface platforms. Marginal bone loss was less pronounced in roughened designs.
Effect of Ultrasonic Bending Vibration Introduced by the L-shaped Ultrasonic Rod on Solidification Structure and Segregation of Large 2A14 Ingots
Authors: Shi Chen , Wu Yongjun , Mao Daheng , Fan Gaofeng
Published: February 2020

In order to achieve long-term and stable ultrasonic treatment in the direct chill semi-continuous casting process, a new L-shaped ceramic ultrasonic wave guide rod is designed to introduce ultrasonic bending vibration into 2A14 aluminum alloy melt. The effect of ultrasonic bending vibration on the solidification structure and composition segregation of large 2A14 aluminum alloy ingots (φ 830 mm × 6000 mm) in the process of semi-continuous casting were studied by means of a direct reading spectrometer, scanning electron microscope, metallographic microscope, and hardness test. The ultrasonic ingot treated by bending vibration was compared with the ingot without ultrasonic treatment and the ingot treated by the traditional straight-rod titanium alloy wave guide rod. The results show that, during the solidification of 2A14 aluminum alloy, ultrasonic treatment can significantly refine the grain, break up the agglomerated secondary phase, and make its distribution uniform. The macro-segregation degree of solute including the negative segregation at the edge of the ingots and the positive segregation in the center can be reduced. Through comparative analysis, the macrostructure of the ingot, treated by the L-shaped ceramic ultrasonic wave guide rod, was found to be better than that of the ingot treated by the traditional straight-rod titanium alloy wave guide rod. In particular, the grain refinement effect at the edge of the ingot was the best, the secondary phase was smaller, more solute elements can be dissolved into the α-Al matrix, and the ability of the L-shaped ultrasonic wave guide rod to restrain segregation was stronger at the edge of the ingot.
Residual Compressive Strength of Short Tubular Steel Columns with Artificially Fabricated Local Corrosion Damage
Authors: Toledo Kyra Kamille , Kim Hyoung-Seok , Jeong Young-Soo , Kim In-Tae
Published: February 2020

Corrosion is considered as one of the main factors in the structural performance deterioration of steel members. In this study, experimental and numerical methods were used to assess the reduction in compressive strength of short tubular steel columns with artificially fabricated local corrosion damage. The corrosion damage was varied with different depths, heights, circumferences, and locations along the column. A parametric numerical study was performed to establish a correlation between the residual compressive strength and the severity of corrosion damage. The results showed that as the corrosion depth, height and circumference increased, the compressive strength decreased linearly. As for the corrosion height, the residual compressive strength became constant after decreasing linearly when the corrosion height was greater than the half-wavelength of buckling of the short columns. An equation is presented to evaluate the residual compressive strength of short columns with local corrosion wherein the volume of the corrosion damage was used as a reduction factor in calculating the compressive strength. The percentage error using the presented equation was found to be within 11.4%.
Comparisons of Diurnal Variations of Land Surface Temperatures from Numerical Weather Prediction Analyses, Infrared Satellite Estimates and In Situ Measurements
Authors: Wang Xiaoni , Prigent Catherine
Published: February 2020

This study evaluates the diurnal cycle of Land Surface Temperature (LST) from Numerical Weather Prediction (NWP) reanalyses (ECMWF ERA5 and ERA Interim), as well as from infrared satellite estimates (ISCCP and SEVIRI/METEOSAT), with in situ measurements. Data covering a full seasonal cycle in 2010 are studied. Careful collocations and cloud filtering are applied. We first compare the reanalysis and satellite products at continental and regional scales, and then we concentrate on comparisons with the in situ observations, under a large variety of environments. SEVIRI shows better agreement with the in situ measurements than the other products, with bias often less than ±2K and correlation of 0.99. Over snow or arid surface, ISCCP tends to have more systematic errors than the other products. ERA5 agrees better to the in situ over barren land than ERA Interim, particularly at night time, thanks to the new surface model. However, over vegetated surfaces, both reanalyses tend to have higher/lower temperature at night/day time than the in situ measurements, probably related to the surface processes and its interactions with atmosphere in the NWP model.
Closed-Loop Control of Droplet Transfer in Electron-Beam Freeform Fabrication
Authors: Chang Shuhe , Zhang Haoyu , Xu Haiying , Sang Xinghua , Wang Li , Du Dong , Chang Baohua
Published: February 2020

In the process of electron-beam freeform fabrication deposition, the surface of the deposit layer becomes rough because of the instability of the feeding wire and the changing of the thermal diffusion condition. This will make the droplet transfer distance change in the deposition process, and the droplet transfer cannot always be stable in the liquid bridge transfer state. It is easy to form a large droplet or make wire and substrate stick together, which makes the deposition quality worsen or even interrupts the deposition process. The current electron-beam freeform fabrication deposition is mostly open-loop control, so it is urgent to realize the real-time and closed-loop control of the droplet transfer and to make it stable in the liquid bridge transfer state. In this paper, a real-time monitoring method based on machine vision is proposed for the droplet transfer of electron-beam freeform fabrication. The detection accuracy is up to ± 0.08 mm. Based on this method, the measured droplet transfer distance is fed back to the platform control system in real time. This closed-loop control system can stabilize the droplet transfer distance within ± 0.14 mm. In order to improve the detection stability of the whole system, a droplet transfer detection algorithm suitable for this scenario has been written, which improves the adaptability of the droplet transfer distance detection method by means of dilatation/erosion, local minimum value suppression, and image segmentation. This algorithm can resist multiple disturbances, such as spatter, large droplet occlusion and so on.
Genetic Algorithm Design of MOF-based Gas Sensor Arrays for CO2-in-Air Sensing
Authors: Day Brian A. , Wilmer Christopher E.
Published: February 2020

Gas sensor arrays, also known as electronic noses, leverage a diverse set of materials to identify the components of complex gas mixtures. Metal-organic frameworks (MOFs) have emerged as promising materials for electronic noses due to their high-surface areas and chemical as well as structural tunability. Using our recently reported genetic algorithm design approach, we examined a set of 50 MOFs and searched through over 1.125 × 1015 unique array combinations to identify optimal arrays for the detection of CO2 in air. We found that despite individual MOFs having lower selectivity for O2 or N2 relative to CO2, intelligently selecting the right combinations of MOFs enables accurate prediction of the concentrations of all components in the mixture (i.e., CO2, O2, N2). We also analyzed the physical properties of the elements in the arrays to develop an intuition for improving array design. Notably, we found that an array whose MOFs have diversity in their volumetric surface areas has improved sensing. Consistent with this observation, we found that the best arrays consistently had greater structural diversity (e.g., pore sizes, void fractions, and surface areas) than the worst arrays.
Are Inductive Current Transformers Performance Really Affected by Actual Distorted Network Conditions? An Experimental Case Study
Authors: Mingotti Alessandro , Peretto Lorenzo , Bartolomei Lorenzo , Cavaliere Diego , Tinarelli Roberto
Published: February 2020

The aim of this work is to assess whether actual distorted conditions of the network are really affecting the accuracy of inductive current transformers. The study started from the need to evaluate the accuracy performance of inductive current transformers in off-nominal conditions, and to improve the related standards. In fact, standards do not provide a uniform set of distorted waveforms to be applied on inductive or low-power instrument transformers. Moreover, there is no agreement yet, among the experts, about how to evaluate the uncertainty of the instrument transformer when the operating conditions are different from the rated ones. To this purpose, the authors collected currents from the power network and injected them into two off-the-shelf current transformers. Then, their accuracy performances have been evaluated by means of the well-known composite error index and an approximated version of it. The obtained results show that under realistic non-rated conditions of the network, the tested transformers show a very good behavior considering their nonlinear nature, arising the question in the title. A secondary result is that the use of the composite error should be more and more supported by the standards, considering its effectiveness in the accuracy evaluation of instrument transformers for measuring purposes.
Percentiles and Reference Values for the Accelerometric Assessment of Static Balance in Women Aged 50–80 Years
Authors: Leirós-Rodríguez Raquel , Romo-Pérez Vicente , García-Soidán Jose Luis , García-Liñeira Jesús
Published: February 2020

The identification of factors that alter postural stability is fundamental in the design of interventions to maintain independence and mobility. This is especially important for women because of their longer life expectancy and higher incidence of falls compared to men. The objective of this study was to construct the percentile box charts and determine the values of reference for the accelerometric assessment of the static balance in women. For this, an observational and cross-sectional study with a sample composed of 496 women (68.8 ± 10.4 years old) was conducted. The measurement of accelerations used a triaxial accelerometer during three tests: two tests on the ground in monopodal support and a test on a mat with monopodal support for 30 s each. In all of the variables, an increase in the magnitude of the accelerations was detected as the age advanced. The box charts of the percentiles of the tests show the amplitude of the interquartile ranges, which increased as the age advanced. The values obtained can be used to assess changes in static balance due to aging, trauma and orthopaedic and neurodegenerative alterations that may alter postural stability and increase the risk of falling.
The OLYMPUS Architecture—Oblivious Identity Management for Private User-Friendly Services
Authors: Torres Moreno Rafael , Bernal Bernabe Jorge , García Rodríguez Jesús , Kasper Frederiksen Tore , Stausholm Michael , Martínez Noelia , Sakkopoulos Evangelos , Ponte Nuno , Skarmeta Antonio
Published: February 2020

Privacy enhancing technologies (PETs) allow to achieve user’s transactions unlinkability across different online Service Providers. However, current PETs fail to guarantee unlinkability against the Identity Provider (IdP), which becomes a single point of failure in terms of privacy and security, and therefore, might impersonate its users. To address this issue, OLYMPUS EU project establishes an interoperable framework of technologies for a distributed privacy-preserving identity management based on cryptographic techniques that can be applied both to online and offline scenarios. Namely, distributed cryptographic techniques based on threshold cryptography are used to split up the role of the Identity Provider (IdP) into several authorities so that a single entity is not able to impersonate or track its users. The architecture leverages PET technologies, such as distributed threshold-based signatures and privacy attribute-based credentials (p-ABC), so that the signed tokens and the ABC credentials are managed in a distributed way by several IdPs. This paper describes the Olympus architecture, including its associated requirements, the main building blocks and processes, as well as the associated use cases. In addition, the paper shows how the Olympus oblivious architecture can be used to achieve privacy-preserving M2M offline transactions between IoT devices.
A Facile Fabrication of a Potentiometric Arrayed Glucose Biosensor Based on Nafion-GOx/GO/AZO
Authors: Chou Jung-Chuan , Lin Si-Hong , Lai Tsu-Yang , Kuo Po-Yu , Lai Chih-Hsien , Nien Yu-Hsun , Su Tzu-Yu
Published: February 2020

In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measured by using the voltage–time (V-T) measurement system. Finally, electrochemical impedance spectroscopy (EIS) was conducted to analyze their charge transfer abilities. The results indicated that the average sensitivity of the glucose biosensor based on Nafion-GOx/GO/AZO was apparently higher than that of the glucose biosensor based on Nafion-GOx/GO/ZnO. In addition, the glucose biosensor based on Nafion-GOx/GO/AZO exhibited an excellent average sensitivity of 15.44 mV/mM and linearity of 0.997 over a narrow range of glucose concentration range, a response time of 26 s, a limit of detection (LOD) of 1.89 mM, and good reproducibility. In terms of the reversibility and stability, the hysteresis voltages (VH) were 3.96 mV and 2.42 mV. Additionally, the glucose biosensor also showed good anti-inference ability and reproducibility. According to these results, it is demonstrated that AZO is a promising material, which could be used to develop a reliable, simple, and low-cost potentiometric glucose biosensor.
Does the Revised Hukou System Facilitate or Restrain the Short-Term Labor Inflows into Chinese Cities?
Authors: Cui Hao , Cho Joonmo
Published: February 2020

According to previous studies, the Chinese revised Hukou system has not been proven to be effective in the short-term movement of the rural labor force or in controlling urban populations. In this study, we address and analyze the self-selection problem from the perspective of inflows of short-term migration from the rural labor force and the endogeneity problem in the adoption of the revised Hukou system, based on the data from China Family Panel Studies (CFPS). The study shows that the revised Hukou system adopted by Chinese local governments was significantly efficient and harmed the short-term migration decisions of the rural labor force.
Evaluation of the Potency of Two Pyolysin-Derived Recombinant Proteins as Vaccine Candidates of Trueperella Pyogenes in a Mouse Model: Pyolysin Oligomerization and Structural Change Affect the Efficacy of Pyolysin-Based Vaccines
Authors: Yang Lingxiao , Liang Hongmin , Wang Bing , Ma Bo , Wang Junwei , Zhang Wenlong
Published: February 2020

Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen in livestock and wild animals. However, only one commercial T. pyogenes vaccine is currently available, and its immunoprotective effect is not ideal. Pyolysin (PLO) is one of the important virulence factors expressed by T. pyogenes and one of the targets for the development of new T. pyogenes vaccines. In this study, we constructed two recombinant antigens, tPLOA1 (contains amino acids 1–110 and domain 4 of the PLO molecule) and tPLOA2 (contains amino acids 190–296 and domain 4 of the PLO molecule). Vaccines were prepared by mixing the two recombinant antigens with incomplete Freund’s adjuvant or sheep red blood cell membrane and provided partial immune protection to immunized mice against the lethal challenge of T. pyogenes. Analysis of the PLO-specific IgG levels of immunized mice indicated that the antibody-inducing potency and immunoprotective efficacy of PLO-based vaccines are affected by the oligomerization and structural changes of PLO after binding to a cholesterol-containing membrane. In addition, the titer of anti-hemolysis antibodies is not a suitable indicator of the immunoprotective effect of these vaccines in PLO-based vaccine-immunized animals. The results provide new insights into the development of T. pyogenes vaccines.
Barriers and enablers to collaborative working between GPs and pharmacists: a qualitative interview study
Authors: Duncan Polly , Ridd Matthew J , McCahon Deborah , Guthrie Bruce , Cabral Christie
Published: February 2020

Background

Many UK GP practices now employ a practice pharmacist, but little is known about how GPs and pharmacists work together to optimise medications for complex patients with multimorbidity.

Aim

To explore GP and pharmacist perspectives on collaborative working within the context of optimising medications for patients with multimorbidity.

Design and setting

A qualitative analysis of semi-structured interviews with GPs and pharmacists working in the West of England, Northern England, and Scotland.

Method

Thirteen GPs and 10 pharmacists were sampled from practices enrolled in the 3D trial (a complex intervention for people with multimorbidity). Participants’ views on collaborative working were explored with interviews that were audiorecorded, transcribed, and analysed thematically. Saturation of data was achieved with no new insights arising from later interviews.

Results

GPs from surgeries that employed a pharmacist tended to value their expertise more than GPs who had not worked with one. Three key themes were identified: resources and competing priorities; responsibility; and professional boundaries. GPs valued pharmacist recommendations that were perceived to improve patient safety, as opposed to those that were technical and unlikely to benefit the patient. Pharmacists who were not known to GPs felt undervalued and wanted feedback from the GPs about their recommendations, particularly those that were not actioned.

Conclusion

A good working relationship between the GP and pharmacist, where each profession understood the other’s skills and expertise, was key. The importance of face-to-face meetings and feedback should be considered in future studies of interdisciplinary interventions, and by GP practices that employ pharmacists and other allied health professionals.