bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of {10–12} Twins on Precipitation Behavior of Extruded Mg–8Al–0.5Zn Alloy During Low-temperature Aging
Authors: Kim Hyun Ji , Kim Ye Jin , Park Sung Hyuk
Published: August 2024

This study investigates the influence of {10–12} twins on the aging behavior of an extruded Mg–8Al–0.5Zn (AZ80) alloy during aging at 150 °C. The extruded AZ80 alloy is subjected to a compressive strain of 6% along the transverse direction and is subsequently aged at 150 °C. The sample with {10–12} twins (referred to as the twinned sample) exhibits a finer grain size and higher internal strain energy compared to the extruded sample. The peak-aging time of the twinned sample is 32 h, which is significantly shorter than that of the extruded sample without twins (200 h). Moreover, the peak-aged hardness of the twinned sample (96.3 Hv) is slightly higher than that of the extruded sample (93.6 Hv), despite the substantially shorter peak-aging time in the former. The high internal strain in the twinned sample, especially within the twins, promotes the formation of continuous precipitates (CPs) during the early stages of aging. As the aging time increases, the number density of fine CPs in the twins increases through additional precipitation. In the extruded sample, the area fraction of discontinuous precipitates (DPs) rapidly increases with increasing aging time. In contrast, the formation and growth of DPs are substantially suppressed in the twinned sample, because CPs predominantly form and twin boundaries act as barriers to DP growth. As a result, the area fraction of the peak-aged twinned sample (5.1%) is significantly lower than that of the peak-aged extruded sample (60.2%). Consequently, the introduction of {10–12} twins in the extruded AZ80 alloy results in the promotion of CP formation, suppression of DP formation, and significant reduction in peak-aging time under the 150 °C aging condition.
MENARASIKAN ULANG LABEL KOLEKSI ARCA CAMUNDI DI PUSAT INFORMASI MAJAPAHIT: SEBUAH TINJAUAN BIOGRAFI BENDA
Authors: Yusuf Muhamad Satok
Published: August 2024

This paper discusses the significance of presenting an educational, holistic, and neutral narrative of a museum collection according to the ICOM 2022 definition of a museum. The Camundi statue, one of the iconic collections of the Majapahit Information Center, is an essential example of museum curatorial bias. Visitors are highly interested in the historical journey of the Camundi statue; however, they lack access to a neutral and holistic information label from the collection. This research emphasises the biography of the Camundi statue's journey by examining the dynamics of the statue's role based on its meaning, research history, and preservation. The study applied a descriptive qualitative approach with the basis of historical archaeology and interpretation according to Igor Kopytoff's concept of the biography of things. The findings reveal ongoing debates among experts regarding the statue's identity and historical journey. The Camundi statue has undergone various transformations in its role, from a revered object of worship to a disaster relic, an item of preservation, and finally, a museum exhibit that should be presented without curatorial bias. The critical evaluation in this study recommends the re-narration of the collection label by considering four aspects of holistic, educative, and neutral narratives
Suggested proof for the Collatz conjecture
Authors: Omran Saleh
Published: August 2024

In the present work, we suggest a proof for 3n+1 problem which was originally introduced by Lothar Collatz in 1937. Collatz conjecture asserts that the function C : N to N; de�fined by C(n) = 3n + 1 if n is odd positive integer number, and C(n) = n/2 if n is even positive integer number goes to 1. We proof that the k-th iterate of Collatz function C^k(n) is bounded for all positive integer numbers k; n and converges to 1.
A deep learning approach to dysphagia-aspiration detecting algorithm through pre- and post-swallowing voice changes
Authors: Kim Jung-Min , Kim Min-Seop , Choi Sun-Young , Lee Kyogu , Ryu Ju Seok
Published: August 2024

Introduction

This study aimed to identify differences in voice characteristics and changes between patients with dysphagia-aspiration and healthy individuals using a deep learning model, with a focus on under-researched areas of pre- and post-swallowing voice changes in patients with dysphagia. We hypothesized that these variations may be due to weakened muscles and blocked airways in patients with dysphagia.

Methods

A prospective cohort study was conducted on 198 participants aged >40 years at the Seoul National University Bundang Hospital from October 2021 to February 2023. Pre- and post-swallowing voice data of the participants were converted to a 64-kbps mp3 format, and all voice data were trimmed to a length of 2 s. The data were divided for 10-fold cross-validation and stored in HDF5 format with anonymized IDs and labels for the normal and aspiration groups. During preprocessing, the data were converted to Mel spectrograms, and the EfficientAT model was modified using the final layer of MobileNetV3 to effectively detect voice changes and analyze pre- and post-swallowing voices. This enabled the model to probabilistically categorize new patient voices as normal or aspirated.

Results

In a study of the machine-learning model for aspiration detection, area under the receiver operating characteristic curve (AUC) values were analyzed across sexes under different configurations. The average AUC values for males ranged from 0.8117 to 0.8319, with the best performance achieved at a learning rate of 3.00e-5 and a batch size of 16. The average AUC values for females improved from 0.6975 to 0.7331, with the best performance observed at a learning rate of 5.00e-5 and a batch size of 32. As there were fewer female participants, a combined model was developed to maintain the sex balance. In the combined model, the average AUC values ranged from 0.7746 to 0.7997, and optimal performance was achieved at a learning rate of 3.00e-5 and a batch size of 16.

Conclusion

This study evaluated a voice analysis-based program to detect pre- and post-swallowing changes in patients with dysphagia, potentially aiding in real-time monitoring. Such a system can provide healthcare professionals with daily insights into the conditions of patients, allowing for personalized interventions.

Clinical Trial Registration:

ClinicalTrials.gov, identifier NCT05149976
Painful ulcerations associated with COVID-19 in an adolescent patient: a case report
Authors: Danesh David O. , Lee Kyulim , Wallihan Rebecca G. , Townsend Janice A. , Mulo Ira , Kumar Ashok
Published: August 2024

Oral lesions associated with SARS-CoV-2 (COVID-19) include aphthous-like ulcers, herpetiform eruption of vesicles and erosions and other findings. Reactive infectious mucocutaneous eruption (RIME) has recently been used to describe non-Mycoplasma pneumoniae pathogens that can lead to rash and mucositis. RIME secondary to SARS-CoV-2 infection is consistent with reports in the literature. The patient in this case report is significant in that it involves only the oral mucosa, although there are cases reported where mucosal involvement is limited to one site. The degree of mucosal involvement in our case report was in the presence of an acute COVID-19 infection without ocular or genital involvement. Oral lesions associated with COVID-19 infection vary in presentation. This paper adds to the understanding of systemic manifestations of COVID-19 infection and provides a reference of clinical findings, management, and interdisciplinary collaboration for caring for this patient.
Causal effects of endometriosis stages and locations on menstruation, ovulation, reproductive function, and delivery modes: a two-sample Mendelian randomization study
Authors: Shen Lin , Li Jie , Zhang Hanwang , Zhao Yiqing
Published: August 2024

Background

Endometriosis is a chronic inflammatory disease of women during their reproductive years. The relationship between the severity and location of endometriosis and menstruation, ovulation, reproductive function, and mode of delivery remains unclear.

Methods

We explored the association between the various phenotypes of endometriosis and menstruation, ovulation, reproductive function, and mode of delivery, using two-sample Mendelian randomization (MR) and summary data on endometriosis stages and locations from the FinnGen consortium and women’s menstruation, ovulation, reproductive function, and mode of delivery from OpenGWAS and ReproGen. Inverse-variance weighting was used for the primary MR analysis. In addition, a series of sensitivity analyses, confounding analyses, co-localization analyses, and multivariate MR analyses were performed.

Results

MR analysis showed a negative effect of moderate to severe endometriosis on age at last live birth (OR = 0.973, 95% CI: 0.960–0.986) and normal delivery (OR = 0.999, 95% CI: 0.998–1.000; values for endpoint were excluded), ovarian endometriosis on age at last live birth (OR = 0.976, 95% CI: 0.965–0.988) and normal delivery (OR = 0.999, 95% CI: 0.998–1.000; values for endpoint were excluded), and fallopian tubal endometriosis on excessive irregular menstruation (OR = 0.966, 95% CI: 0.942–0.990). Bidirectional MR analysis showed that age at menarche had a negative causal effect on intestinal endometriosis (OR = 0.417, 95% CI: 0.216–0.804). All MR analyses were confirmed by sensitivity analyses, and only the genetic effects of moderate to severe endometriosis on normal delivery and age at last live birth were supported by co-localization evidence.

Conclusion

Our findings deepen the understanding of the relationship between various types of endometriosis and menstruation, ovulation, reproductive function, and mode of delivery and clarify the important role of moderate to severe endometriosis.
Bat humoral immunity and its role in viral pathogenesis, transmission, and zoonosis
Authors: Roffler Anne A. , Maurer Daniel P. , Lunn Tamika J. , Sironen Tarja , Forbes Kristian M. , Schmidt Aaron G.
Published: August 2024

Bats harbor viruses that can cause severe disease and death in humans including filoviruses (e.g., Ebola virus), henipaviruses (e.g., Hendra virus), and coronaviruses (e.g., SARS-CoV). Bats often tolerate these viruses without noticeable adverse immunological effects or succumbing to disease. Previous studies have largely focused on the role of the bat’s innate immune response to control viral pathogenesis, but little is known about bat adaptive immunity. A key component of adaptive immunity is the humoral response, comprised of antibodies that can specifically recognize viral antigens with high affinity. The antibody genes within the 1,400 known bat species are highly diverse, and these genetic differences help shape fundamental aspects of the antibody repertoire, including starting diversity and viral antigen recognition. Whether antibodies in bats protect, mediate viral clearance, and prevent transmission within bat populations is poorly defined. Furthermore, it is unclear how neutralizing activity and Fc-mediated effector functions contribute to bat immunity. Although bats have canonical Fc genes (e.g., mu, gamma, alpha, and epsilon), the copy number and sequences of their Fc genes differ from those of humans and mice. The function of bat antibodies targeting viral antigens has been speculated based on sequencing data and polyclonal sera, but functional and biochemical data of monoclonal antibodies are lacking. In this review, we summarize current knowledge of bat humoral immunity, including variation between species, their potential protective role(s) against viral transmission and replication, and address how these antibodies may contribute to population dynamics within bats communities. A deeper understanding of bat adaptive immunity will provide insight into immune control of transmission and replication for emerging viruses with the potential for zoonotic spillover.
Intra- and inter-host origin, evolution dynamics and spatial-temporal transmission characteristics of circoviruses
Authors: Cui Yongqiu , Li Siting , Xu Weiying , Xie Jiali , Wang Dedong , Hou Lei , Zhou Jianwei , Feng Xufei , Liu Jue
Published: August 2024

Introduction

Since their identification in 1974, circoviruses have caused clinicopathological diseases in various animal species, including humans. However, their origin, transmission, and genetic evolution remain poorly understood.

Methods

In this study, the genome sequences of circovirus were obtained from GenBank, and the Bayesian stochastic search variable selection algorithm was employed to analyzed the evolution and origin of circovirus.

Results

Here, the evolutionary origin, mode of transmission, and genetic recombination of the circovirus were determined based on the available circovirus genome sequences. The origin of circoviruses can be traced back to fish circovirus, which might derive from fish genome, and human contributes to transmission of fish circovirus to other species. Furthermore, mosquitos, ticks, bats, and/or rodents might play a role as intermediate hosts in circovirus intra- and inter-species transmission. Two major lineages (A and B) of circoviruses are identified, and frequent recombination events accelerate their variation and spread. The time to the most recent common ancestor of circoviruses can be traced back to around A.D. 600 and has been evolving at a rate of 10-4 substitutions site-1 year-1 for a long time.

Discussion

These comprehensive findings shed light on the evolutionary origin, population dynamics, transmission model, and genetic recombination of the circovirus providing valuable insights for the development of prevention and control strategies against circovirus infections.
Cellular senescence in the pathogenesis of pulmonary arterial hypertension: the good, the bad and the uncertain
Authors: Safaie Qamsari Elmira , Stewart Duncan J.
Published: August 2024

Senescence refers to a cellular state marked by irreversible cell cycle arrest and the secretion of pro-inflammatory and tissue-remodeling factors. The senescence associated secretory phenotype (SASP) impacts the tissue microenvironment and provides cues for the immune system to eliminate senescent cells (SCs). Cellular senescence has a dual nature; it can be beneficial during embryonic development, tissue repair, and tumor suppression, but it can also be detrimental in the context of chronic stress, persistent tissue injury, together with an impairment in SC clearance. Recently, the accumulation of SCs has been implicated in the pathogenesis of pulmonary arterial hypertension (PAH), a progressive condition affecting the pre-capillary pulmonary arterial bed. PAH is characterized by endothelial cell (EC) injury, inflammation, and proliferative arterial remodeling, which leads to right heart failure and premature mortality. While vasodilator therapies can improve symptoms, there are currently no approved treatments capable of reversing the obliterative arterial remodeling. Ongoing endothelial injury and dysfunction is central to the development of PAH, perpetuated by hemodynamic perturbation leading to pathological intimal shear stress. The precise role of senescent ECs in PAH remains unclear. Cellular senescence may facilitate endothelial repair, particularly in the early stages of disease. However, in more advanced disease the accumulation of senescent ECs may promote vascular inflammation and occlusive arterial remodeling. In this review, we will examine the evidence that supports a role of endothelial cell senescence to the pathogenesis of PAH. Furthermore, we will compare and discuss the apparent contradictory outcomes with the use of interventions targeting cellular senescence in the context of experimental models of pulmonary hypertension. Finally, we will attempt to propose a framework for the understanding of the complex interplay between EC injury, senescence, inflammation and arterial remodeling, which can guide further research in this area and the development of effective therapeutic strategies.
Role of the type 3 cytokines IL-17 and IL-22 in modulating metabolic dysfunction-associated steatotic liver disease
Authors: Abdelnabi Mohamed N. , Hassan Ghada S. , Shoukry Naglaa H.
Published: August 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) comprises a spectrum of liver diseases that span simple steatosis, metabolic dysfunction-associated steatohepatitis (MASH) and fibrosis and may progress to cirrhosis and cancer. The pathogenesis of MASLD is multifactorial and is driven by environmental, genetic, metabolic and immune factors. This review will focus on the role of the type 3 cytokines IL-17 and IL-22 in MASLD pathogenesis and progression. IL-17 and IL-22 are produced by similar adaptive and innate immune cells such as Th17 and innate lymphoid cells, respectively. IL-17-related signaling is upregulated during MASLD resulting in increased chemokines and proinflammatory cytokines in the liver microenvironment, enhanced recruitment of myeloid cells and T cells leading to exacerbation of inflammation and liver disease progression. IL-17 may also act directly by activating hepatic stellate cells resulting in increased fibrosis. In contrast, IL-22 is a pleiotropic cytokine with a dominantly protective signature in MASLD and is currently being tested as a therapeutic strategy. IL-22 also exhibits beneficial metabolic effects and abrogates MASH-related inflammation and fibrosis development via inducing the production of anti-oxidants and anti-apoptotic factors. A sex-dependent effect has been attributed to both cytokines, most importantly to IL-22 in MASLD or related conditions. Altogether, IL-17 and IL-22 are key effectors in MASLD pathogenesis and progression. We will review the role of these two cytokines and cells that produce them in the development of MASLD, their interaction with host factors driving MASLD including sexual dimorphism, and their potential therapeutic benefits.
Measurement of cervical grainy sandy patches, homogeneous yellow sandy patches, and abnormal blood vessels proportions in women with female genital schistosomiasis - a clinical validation study of a digital gridded imaging technique
Authors: Dragsbæk Sofie J. , Carstens Sofie K. , Savkov Ida , Jøker Karoline , Randrianasolo Bodo S. , Leutscher Peter D. , Arenholt Louise
Published: August 2024

Background

Female genital schistosomiasis (FGS) is characterised by cervico-vaginal lesions such as grainy sandy patches, homogeneous yellow sandy patches, and abnormal blood vessels. Diagnosis, treatment, and surveillance of FGS presents challenges due to the absence of diagnostic tools in endemic areas. Likewise, a tool for quantification of the lesions is missing. This study aimed to validate the digital gridded imaging technique (DGIT) for quantification of three specific cervical lesion types associated with FGS.

Methods

Using the QubiFier software program, 70 cervical photographic images obtained from women diagnosed with FGS and treated with Praziquantel (PZQ) were randomly sampled for a validation study. The women lived in a S. haematobium-endemic area of Madagascar. For each image, a semi-transparent grid was applied, composed of 424 equally sized squares positioned to cover the cervix. Squares exhibiting any of the specific lesions were marked by three observers to determine the grainy sandy patches proportion (GSP), homogeneous yellow sandy patches proportion (HSP), and abnormal blood vessel proportion (BVP). To train the observers, a set of ten images was utilized, leaving 60 images for the validation. To test inter-rater reliability, the 60 cervical images were scored independently by three observers. To test intra-rater reliability, ten images were scored twice with a two-week interval. Intraclass correlation coefficient (ICC) was used as the main statistical method to assess the reliability between observers.

Results

The median age of the included women was 26.5 (IQR 20.8-33.0) years, and 74% of them had detectableS. haematobiumeggs in their urine. The three proportions were found to be consistent and reliable across the observers, as well as the rescoring on Days 0 and 14. Inter-rater reliability was good for all three cervical lesion types (ICC 0.768-0.890). Intra-rater reliability was good for GSP (ICC 0.832) and excellent for HSP and BVP (ICC 0.932 and 0.982, respectively).

Conclusion

In this study DGIT was validated as a potential morbidity detection method for quantification of the three lesion types associated with FGS.
Paleoradiological and scientific investigations of the screaming woman mummy from the area beneath Senmut’s (1479–1458 BC) Theban tomb (TT71)
Authors: Saleem Sahar N. , El-Merghani Samia
Published: August 2024

Introduction

The Screaming Mummy of Cairo Egyptian-Museum Store, is an anonymous woman with a wide-open mouth coded as CIT8, discovered beneath Theban Tomb 71 (TT71) which is the burial site of Senmut’s relatives, the architect of 18th-Dynasty Queen Hatschepsut (1479–1458 BC). The study aims to evaluate if combining computed tomography (CT) with scientific investigations and archeological data of the Screaming Mummy CIT8 will reveal information about its physical appearance, health, cause of death, and mummification.

Methods

We CT-scanned the mummy and created reconstructed images. Scanning-Electron-Microscope (SEM), Fourier-Transform-Infrared-Spectroscopy (FTIR), and X-ray-Diffraction-Analysis (XRD) were used to investigate mummy skin, hair, and wig samples. We compared our findings to previous data.

Results

Computed tomography estimated the age of death to be 48.1 years ±14.6 based on the pubic symphyseal surface. CT detected mild-to-moderate teeth attrition, and joints degeneration. The desiccated brain and viscera remained in situ. FTIR revealed the wig is formed of midrib date palm that shows in CT as spiral low density fibers. The wig fibers are partially coated with a thick substance that is inspected as black consolidation and identified as crystalline by XRD, comparable to material found in an ancient wig-making workshop. FTIR showed that the skin, hair, and wig samples were treated with imported juniper resin had anti-bacterial and insecticidal properties. The skin and wig samples contained frankincense, and the hair sample contained henna.

Discussion

Combining the advantages of paleoradiology to the scientific investigations, provided enhanced comprehension of the mummy CIT8 and ancient Egyptian wig structure and material. CT scanning non-invasively showed the mummy’s inner and exterior morphology, and estimated the age of death as 48 years. CT evaluated the mummification technique based on retained viscera and absence of embalming packs. The scientific tests revealed expensive imported embalming materials, contradicting the traditional belief that the non-removal of the viscera implied poor mummification, resulting in careless embalmers sealing the mouth. The widely opened mouth could be a result of facial expression of suffering before death, fixed by cadaveric spasm. The study also explores how rigor mortis, tissue decomposition, burial techniques, and postmortem alterations may contribute to a mummy’s screaming appearance.
Targeting gut-microbiota for gastric cancer treatment: a systematic review
Authors: Marashi Amir , Hasany Saina , Moghimi Sadra , Kiani Reza , Mehran Asl Sina , Dareghlou Yasamin Alavi , Lorestani Parsa , Varmazyar Shirin , Jafari Fatemeh , Ataeian Shakiba , Naghavi Kiana , Sajjadi Seyed Mohammad , Haratian Negar , Alinezhad Arman , Azhdarimoghaddam Aida , Sadat Rafiei Seyed Kiarash , Anar Mahsa Asadi
Published: August 2024

Background

Preclinical research has identified the mechanisms via which bacteria influence cancer treatment outcomes. Clinical studies have demonstrated the potential to modify the microbiome in cancer treatment. Herein, we systematically analyze how gut microorganisms interact with chemotherapy and immune checkpoint inhibitors, specifically focusing on how gut bacteria affect the pharmacokinetics and pharmacodynamics of cancer treatment.

Method

This study searched Web of Science, Scopus, and PubMed until August 2023. Studies were screened by their title and abstract using the Rayyan intelligent tool for systematic reviews. Quality assessment of studies was done using the JBI critical appraisal tool.

Result

Alterations in the gut microbiome are associated with gastric cancer and precancerous lesions. These alterations include reduced microbial alpha diversity, increased bacterial overgrowth, and decreased richness and evenness of gastric bacteria. Helicobacter pylori infection is associated with reduced richness and evenness of gastric bacteria, while eradication only partially restores microbial diversity. The gut microbiome also affects the response to cancer treatments, with higher abundances of Lactobacillus associated with better response to anti-PD-1/PD-L1 immunotherapy and more prolonged progression-free survival. Antibiotic-induced gut microbiota dysbiosis can reduce the anti-tumor efficacy of 5-Fluorouracil treatment, while probiotics did not significantly enhance it. A probiotic combination containing Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis, and Bacillus cereus can reduce inflammation, enhance immunity, and restore a healthier gut microbial balance in gastric cancer patients after partial gastrectomy.

Conclusion

Probiotics and targeted interventions to modulate the gut microbiome have shown promising results in cancer prevention and treatment efficacy.

Systematic review registration:https://osf.io/6vcjp.
Heterogeneity in the projections and excitability of tyraminergic/octopaminergic neurons that innervate the Drosophila reproductive tract
Authors: Rohrbach Ethan W. , Asuncion James D. , Meera Pratap , Kralovec Mason , Deshpande Sonali A. , Schweizer Felix E. , Krantz David E.
Published: August 2024

Aminergic nuclei in mammals are generally composed of relatively small numbers of cells with broad projection patterns. Despite the gross similarity of many individual neurons, recent transcriptomic, anatomic and behavioral studies suggest previously unsuspected diversity. Smaller clusters of aminergic neurons in the model organism Drosophila melanogaster provide an opportunity to explore the ramifications of neuronal diversity at the level of individual cells. A group of approximately 10 tyraminergic/octopaminergic neurons innervates the female reproductive tract in flies and has been proposed to regulate multiple activities required for fertility. The projection patterns of individual neurons within the cluster are not known and it remains unclear whether they are functionally heterogenous. Using a single cell labeling technique, we show that each region of the reproductive tract is innervated by a distinct subset of tyraminergic/octopaminergic cells. Optogenetic activation of one subset stimulates oviduct contractions, indicating that the cluster as a whole is not required for this activity, and underscoring the potential for functional diversity across individual cells. Using whole cell patch clamp, we show that two adjacent and morphologically similar cells are tonically inhibited, but each responds differently to injection of current or activation of the inhibitory GluCl receptor. GluCl appears to be expressed at relatively low levels in tyraminergic/octopaminergic neurons within the cluster, suggesting that it may regulate their excitability via indirect pathways. Together, our data indicate that specific tyraminergic/octopaminergic cells within a relatively homogenous cluster have heterogenous properties and provide a platform for further studies to determine the function of each cell.
Multiparametric MRI radiomics for predicting disease-free survival and high-risk histopathological features for tumor recurrence in endometrial cancer
Authors: Renton Mary , Fakhriyehasl Mina , Weiss Jessica , Milosevic Michael , Laframboise Stephane , Rouzbahman Marjan , Han Kathy , Jhaveri Kartik
Published: August 2024

Background

Current preoperative imaging is insufficient to predict survival and tumor recurrence in endometrial cancer (EC), necessitating invasive procedures for risk stratification.

Purpose

To establish a multiparametric MRI radiomics model for predicting disease-free survival (DFS) and high-risk histopathologic features in EC.

Methods

This retrospective study included 71 patients with histopathology-proven EC and preoperative MRI over a 10-year period. Clinicopathology data were extracted from health records. Manual MRI segmentation was performed on T2-weighted (T2W), apparent diffusion coefficient (ADC) maps and dynamic contrast-enhanced T1-weighted images (DCE T1WI). Radiomic feature (RF) extraction was performed with PyRadiomics. Associations between RF and histopathologic features were assessed using logistic regression. Associations between DFS and RF or clinicopathologic features were assessed using the Cox proportional hazards model. All RF with univariate analysis p-value < 0.2 were included in elastic net analysis to build radiomic signatures.

Results

The 3-year DFS rate was 68% (95% CI = 57%-80%). There were no significant clinicopathologic predictors for DFS, whilst the radiomics signature was a strong predictor of DFS (p<0.001, HR 3.62, 95% CI 1.94, 6.75). From 107 RF extracted, significant predictive elastic net radiomic signatures were established for deep myometrial invasion (p=0.0097, OR 4.81, 95% CI 1.46, 15.79), hysterectomy grade (p=0.002, OR 5.12, 95% CI 1.82, 14.45), hysterectomy histology (p=0.0061, OR 18.25, 95% CI 2.29,145.24) and lymphovascular space invasion (p<0.001, OR 5.45, 95% CI 2.07, 14.36).

Conclusion

Multiparametric MRI radiomics has the potential to create a non-invasive a priori approach to predicting DFS and high-risk histopathologic features in EC.
Role of poly-ADP-ribose polymerase inhibitors after brain progression in platinum-sensitive ovarian cancer: a case report and review of the literature
Authors: Lendinez-Sanchez Gonzalo , Diaz-Redondo Tamara , Iglesias-Campos Marcos , Garrido-Almazán Lucía , Alba-Conejo Emilio , Rueda-Dominguez Antonio , Sanchez-Muñoz Alfonso
Published: August 2024

Introduction

The incidence of brain metastases in ovarian cancer is quite rare, being approximately 1%–2%. According to retrospective studies, patients with BRCA 1/2 mutations present a higher risk. The trimodal approach based on surgery, radiotherapy, and chemotherapy presents better outcomes, but the prognosis remains poor with overall survival since the brain progression is around 1 year. Poly-ADP-ribose polymerase inhibitors (PARPi) have provided a new alternative for the management of advanced ovarian cancer. The SOLO2, NOVA, and ARIEL3 clinical trials do not refer data on patients with brain metastases, and the published evidence for PARPi in this setting comes only from case reports and retrospective studies.

Case report

We present the case of a 54-year-old woman with stage IV ovarian high-grade serous papillary carcinoma who, after 37 months of treatment with olaparib, presented a single brain lesion. After radical treatment with surgery and adjuvant whole-brain radiotherapy, she resumed olaparib with no evidence of disease during 15 months. After a second single brain relapse treated with stereotactic radiosurgery, the patient continued olaparib beyond the brain progression with no evidence of extracranial disease. Despite that there were no changes in size or number of brain lesions, the neurological situation progressively worsened and the patient died 8 months after the second progression.

Discussion

The higher incidence of brain metastases of ovarian cancer points out a possible tropism for the CNS in BRCA-mutated patients. In preclinical studies, PARPi has shown to cross the blood–brain barrier, with possible antitumor activity in the central nervous system (CNS) while maintaining control of extracranial disease. The best survival data are obtained with a trimodal approach, and adding a PARPi could improve the survival outcomes in the context of platinum-sensitivity disease. Targeted therapies combined with local treatments are also used in other malignancies, suggesting potential effectiveness due to tumor heterogeneity. PARPi before brain metastasis may delay its diagnosis, and using iPARP after brain metastases could improve the outcome of this population.

Conclusion

The role that PARPi may have in the treatment of brain metastases of ovarian cancer requires more studies. In the context of radical treatment of brain metastasis (surgery and/or RT), with no evidence of extracranial disease, maintaining treatment with PARPi beyond the brain progression should be considered.
Natural herbal extract roles and mechanisms in treating cerebral ischemia: A systematic review
Authors: Yang Jiashuo , Yu Bo , Zheng Jian
Published: August 2024

Background

Stroke has been the focus of medical research due to its serious consequences and sequelae. Among the tens of millions of new stroke patients every year, cerebral ischemia patients account for the vast majority. While cerebral ischemia drug research and development is still ongoing, most drugs are terminated at preclinical stages due to their unacceptable toxic side effects. In recent years, natural herbs have received considerable attention in the pharmaceutical research and development field due to their low toxicity levels. Numerous studies have shown that natural herbs exert actions that cannot be ignored when treating cerebral ischemia.

Methods

We reviewed and summarized the therapeutic effects and mechanisms of different natural herbal extracts on cerebral ischemia to promote their application in this field. We used keywords such as “natural herbal extract,” “herbal medicine,” “Chinese herbal medicine” and “cerebral ischemia” to comprehensively search PubMed, ScienceDirect, ScienceNet, CNKI, and Wanfang databases, after which we conducted a detailed screening and review strategy.

Results

We included 120 high-quality studies up to 10 January 2024. Natural herbal extracts had significant roles in cerebral ischemia treatments via several molecular mechanisms, such as improving regional blood flow disorders, protecting the blood-brain barrier, and inhibiting neuronal apoptosis, oxidative stress and inflammatory responses.

Conclusion

Natural herbal extracts are represented by low toxicity and high curative effects, and will become indispensable therapeutic options in the cerebral ischemia treatment field.
Influence of protocol variables on outcomes of the star excursion balance test group (SEBT, mSEBT, YBT-LQ) in healthy individuals: a systematic review
Authors: Zając Bartosz , Olszewski Maciej , Mika Anna
Published: August 2024

Background

The “SEBT group,” which includes the Star Excursion Balance Test (SEBT), its modified version (mSEBT), and the Lower Quarter Y-Balance Test (YBT-LQ), is used to assess the limits of stability. Interestingly, the testing protocol allows users a considerable degree of flexibility, which can affect the obtained results. Therefore, the objective of this systematic review was to analyze the impact of different protocol variants within the “SEBT group” on outcomes.

Methods

Data were acquired by searching 4 databases (MEDLINE, ScienceDirect, Wiley, Springer Link) focusing on studies published in English in peer-reviewed journals, empirical in nature, conducted on healthy individuals, and examining the effects of various protocol variants on test outcomes. Study quality was assessed with the NHLBI quality assessment tool for pre-post studies with no control group.

Results

The calculation method based on the maximum repetition yields statistically significantly higher results compared to other calculation methods. Allowing unrestricted arm movements during the test results in statistically significantly higher scores compared to the procedure that restricts arm movements. The impact of a warm-up, wearing footwear during testing, and using a dedicated kit remains ambiguous. To obtain reliable results, 4–6 familiarization trials are necessary, though fewer may suffice for athletes experienced in performing the test.

Conclusion

This systematic review highlights the significant impact of the calculation method and arm movement restrictions on the outcomes of the “SEBT group.” The effects of wearing footwear during testing, warm-up, and using a dedicated test kit remain unclear. The required number of familiarization repetitions may varies depending on biological maturity level of the person being tested. Future research should develop a warm-up protocol tailored to the needs of the “SEBT group,” and investigate the impact of heel elevation during testing on outcomes.

Systematic review registration

The protocol for this systematic review was prospectively registered in the OSF Registries (https://doi.org/10.17605/OSF.IO/JSKH2).
Dual functionality of pathogenesis-related proteins: defensive role in plants versus immunosuppressive role in pathogens
Authors: Han Zhu , Schneiter Roger
Published: August 2024

Plants respond to pathogen exposure by activating the expression of a group of defense-related proteins known as Pathogenesis-Related (PR) proteins, initially discovered in the 1970s. These PR proteins are categorized into 17 distinct families, denoted as PR1-PR17. Predominantly secreted, most of these proteins execute their defensive roles within the apoplastic space. Several PR proteins possess well-defined enzymatic functions, such as β-glucanase (PR2), chitinases (PR3, 4, 8, 11), proteinase (PR7), or RNase (PR10). Enhanced resistance against pathogens is observed upon PR protein overexpression, while their downregulation renders plants more susceptible to pathogen infections. Many of these proteins exhibit antimicrobial activity in vitro, and due to their compact size, some are classified as antimicrobial peptides. Recent research has unveiled that phytopathogens, including nematodes, fungi, and phytophthora, employ analogous proteins to bolster their virulence and suppress plant immunity. This raises a fundamental question: how can these conserved proteins act as antimicrobial agents when produced by the host plant but simultaneously suppress plant immunity when generated by the pathogen? In this hypothesis, we investigate PR proteins produced by pathogens, which we term “PR-like proteins,” and explore potential mechanisms by which this class of virulence factors operate. Preliminary data suggests that these proteins may form complexes with the host’s own PR proteins, thereby interfering with their defense-related functions. This analysis sheds light on the intriguing interplay between plant and pathogen-derived PR-like proteins, providing fresh insights into the intricate mechanisms governing plant-pathogen interactions.
Comparative transcriptome analysis of resistant and susceptible watermelon genotypes reveals the role of RNAi, callose, proteinase, and cell wall in squash vein yellowing virus resistance
Authors: Kumar Rahul , Chanda Bidisha , Adkins Scott , Kousik Chandrasekar S.
Published: August 2024

Watermelon (Citrullus lanatus) is the third largest fruit crop in the world in term of production. However, it is susceptible to several viruses. Watermelon vine decline (WVD), caused by whitefly-transmitted squash vein yellowing virus (SqVYV), is a disease that has caused over $60 million in losses in the US and continues to occur regularly in southeastern states. Understanding the molecular mechanisms underlying resistance to SqVYV is important for effective disease management. A time-course transcriptomic analysis was conducted on resistant (392291-VDR) and susceptible (Crimson Sweet) watermelon genotypes inoculated with SqVYV. Significantly higher levels of SqVYV were observed over time in the susceptible compared to the resistant genotype. The plasmodesmata callose binding protein (PDCB) gene, which is responsible for increased callose deposition in the plasmodesmata, was more highly expressed in the resistant genotype than in the susceptible genotype before and after inoculation, suggesting the inhibition of cell-to-cell movement of SqVYV. The potential role of the RNA interference (RNAi) pathway was observed in the resistant genotype based on differential expression of eukaryotic initiation factor (eIF), translin, DICER, ribosome inactivating proteins, RNA-dependent RNA polymerase (RDR), and Argonaute (AGO) genes after inoculation. The significant differential expression of hormone-related genes, including those involved in the ethylene, jasmonic acid, auxin, cytokinin, gibberellin, and salicylic acid signaling pathways, was observed, emphasizing their regulatory roles in the defense response. Genes regulating pectin metabolism, cellulose synthesis, cell growth and development, xenobiotic metabolism, and lignin biosynthesis were overexpressed in the susceptible genotype, suggesting that alterations in cell wall integrity and growth processes result in disease symptom development. These findings will be helpful for further functional studies and the development of SqVYV-resistant watermelon cultivars.
Effect of formulations and adjuvants on the properties of acetamiprid solution and droplet deposition characteristics sprayed by UAV
Authors: Zeeshan Muhammad , Li Haoran , Yousaf Gulfam , Ren Hao , Liu Yapeng , Arshad Muhammad , Dou Zechen , Han Xiaoqiang
Published: August 2024

While the pesticide formulations are widely used for pest control, the combined effects of these formulations with adjuvants on droplet behavior, spraying characteristics, and pest control still need to be studied. To clarify their impact on droplet behavior, spraying characteristics, and control efficacy, six formulations of acetamiprid and six adjuvants were examined. A series of laboratory and field experiments were conducted to analyze the physicochemical properties, toxicity against cotton aphids, droplet deposition characteristics, and droplet drift. The results indicated that 5% acetamiprid micro-emulsion (ME) enhanced the physicochemical features and effectiveness in pest control compared to other formulations. The nongjianfei considerably enhanced the efficiency of all acetamiprid formulations when added. The addition of selected adjuvants to pesticide formulations improved the performance of certain physicochemical properties such as viscosity and surface tension and led to higher aphid mortality rates, demonstrating enhanced pest control effectiveness during the present study. In the field experiments, the combination effect of acetamiprid formulations and adjuvants exhibited a higher droplet size, coverage, and density within the cotton canopy. However, 5% acetamiprid ME was found to be most effective followed by nongjianfei. Furthermore, 5% acetamiprid ME with adjuvant reduced the droplet drift and provided better deposition when compared with other formulations. Overall, the combination of specific formulations and adjuvants led to improved physicochemical properties, enhanced droplet deposition characteristics, reduced spray drift, and increased pesticide deposition. These findings highlighted the significance of selecting appropriate pesticide formulations and adjuvants and provided a solid foundation for efficient pesticide spraying through UAVs.
Chromosome-level assembly of Lindenbergia philippensis and comparative genomic analyses shed light on genome evolution in Lamiales
Authors: Chen Bao-Zheng , Li Da-Wei , Luo Kai-Yong , Jiu Song-Tao , Dong Xiao , Wang Wei-Bin , Li Xu-Zhen , Hao Ting-Ting , Lei Ya-Hui , Guo Da-Zhong , Liu Xu-Tao , Duan Sheng-Chang , Zhu Yi-Fan , Chen Wei , Dong Yang , Yu Wen-Bin
Published: August 2024

Lamiales, comprising over 23,755 species across 24 families, stands as a highly diverse and prolific plant group, playing a significant role in the cultivation of horticultural, ornamental, and medicinal plant varieties. Whole-genome duplication (WGD) and its subsequent post-polyploid diploidization (PPD) process represent the most drastic type of karyotype evolution, injecting significant potential for promoting the diversity of this lineage. However, polyploidization histories, as well as genome and subgenome fractionation following WGD events in Lamiales species, are still not well investigated. In this study, we constructed a chromosome-level genome assembly of Lindenbergia philippensis (Orobanchaceae) and conducted comparative genomic analyses with 14 other Lamiales species. L. philippensis is positioned closest to the parasitic lineage within Orobanchaceae and has a conserved karyotype. Through a combination of Ks analysis and syntenic depth analysis, we reconstructed and validated polyploidization histories of Lamiales species. Our results indicated that Primulina huaijiensis underwent three rounds of diploidization events following the γ-WGT event, rather than two rounds as reported. Besides, we reconfirmed that most Lamiales species shared a common diploidization event (L-WGD). Subsequently, we constructed the Lamiales Ancestral Karyotype (LAK), comprising 11 proto-chromosomes, and elucidated its evolutionary trajectory, highlighting the highly flexible reshuffling of the Lamiales paleogenome. We identified biased fractionation of subgenomes following the L-WGD event across eight species, and highlighted the positive impacts of non-WGD genes on gene family expansion. This study provides novel genomic resources and insights into polyploidy and karyotype remodeling of Lamiales species, essential for advancing our understanding of species diversification and genome evolution.
Different effects of visual occlusion on interpersonal coordination of head and body movements during dyadic conversations
Authors: Kodama Kentaro , Shimizu Daichi , Fujiwara Ken
Published: August 2024

Introduction

In recent decades, interpersonal coordination and synchrony have been extensively examined in the field of psychology and cognitive science. Studies suggest that perceptual information enables interpersonal coordination and that perceptual noise may even enhance coordination. However, how these perceptual factors influence interpersonal coordination dynamics between head and body movements remains unclear. This study investigated the effect of visual information on the interpersonal coordination of head and body movements during dyadic conversations.

Methods

The availability of visual information was manipulated by positioning a partition halfway between a pair of participants, and the conversations were recorded using a video camera. A video-based human pose estimation software (OpenPose) was used to quantify each interlocutor’s head and body movements, which were submitted for cross-recurrence quantification analysis (CRQA), to assess the degree of coordination between the interlocutors.

Results

The results showed different effects between head- and body-movement coordination (i.e., a CRQA measure, maximum line length). The occlusion of visual information increased head-movement coordination, whereas it decreased body-movement coordination.

Discussion

The results suggest that a distinct mechanism may be present at the head- and body-movement coordination level and this study observed differing appearances of compensatory behaviors. Further studies should be conducted to investigate the complex relationships between interpersonal coordination dynamics and various kinds of communication constraints, such as long-term or short-term, and lower-order (perceptual-motor) or higher-order (cognitive-social) level constraints.