bluuebunny/crossref_metadata_2025 huggingface dataset:
Defining Suitable Reference Genes for qRT-PCR in Plagiodera versicolora (Coleoptera: Chrysomelidae) under Different Biotic or Abiotic Conditions
Authors: Tu Chengjie , Xu Pei , Han Runhua , Luo Jing , Xu Letian
Published: May 2022

Plagiodera versicolora (Coleoptera: Chrysomelidae) is one of the most destructive pests of the Salicaceae worldwide, which has established complex interactions with surrounding organisms. Uncovering the molecular mechanisms of some antagonistic interactions would facilitate the development of environmentally friendly pest insect management strategies. Suitable reference genes are essential for reliable qPCR and gene expression analysis in molecular studies; however, a comprehensive assessment of reference genes in P. versicolora is still lacking. In this study, the stability of seven housekeeping genes (including Actin, EF1A, α-tubulin, RPL13a, RPS18, RPL8 and UBC) was investigated under both biotic (developmental stages, tissues, sex and pathogen treatment) and abiotic (RNA interference treatment, temperature treatment) conditions. The geNorm, NormFinder, BestKeeper, and ΔCt programs were used to analyze gene expression data. The RefFinder synthesis analysis was applied to suggest a handful of appropriate reference genes for each experimental condition. RPS18 and EF1A were the most reliable reference genes in different development stages; RPS18 and RPL8 were most stable in female and male adults, different tissues, different temperatures, and pathogen treatment; α-tubulin and RPL13a were most stable after dietary RNAi treatment. The research provides a strong basis for future research into the molecular biology of P. versicolora.
Direct Urine Resistance Detection Using VITEK 2
Authors: Torres-Sangiao Eva , Lamas Rodriguez Brais , Cea Pájaro María , Carracedo Montero Raquel , Parajó Pazos Noelia , García-Riestra Carlos
Published: May 2022

Urinary tract infections (UTIs) are the most common infectious diseases in both communities and hospitals. With non-anatomical or functional abnormalities, UTIs are usually self-limiting, though women suffer more reinfections throughout their lives. Certainly, antibiotic treatment leads to a more rapid resolution of symptoms, but also it selects resistant uropathogens and adversely affects the gut and vaginal microbiota. As uropathogens are increasingly becoming resistant to currently available antibiotics, it could be time to explore alternative strategies for managing UTIs. Rapid identification and antimicrobial susceptibility testing (AST) allow fast and precise treatment. The objective of this study was to shorten the time of diagnosis of UTIs by combining pathogen screening through flow cytometry, microbial identification by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS), and the VITEK 2 system for the direct analysis of urine samples. First, we selected positive urine samples by flow cytometry using UF5000, establishing the cut-off for positive at 150 bacteria/mL. After confirming the identification using MALDI-TOF MS and filtering the urine samples for Escherichia coli, we directly tested the AST N388 card using VITEK 2. We tested a total of 211 E. coli from urine samples. Cefoxitin, ertapenem, imipenem, gentamicin, nalidixic acid, ciprofloxacin, fosfomycin, and nitrofurantoin had no major important errors (MIE), and ampicillin, cefuroxime, and tobramycin showed higher MIEs. Cefepime, imipenem, and tobramycin had no major errors (ME). Fosfomycin was the antibiotic with the most MEs. The antibiotic with the most minor errors (mE) was ceftazidime. The total categorical agreement (CA) was 97.4% with a 95% CI of (96.8–97.9)95%. The direct AST from the urine samples proposed here was shorter by one day, without significant loss of sensibility regarding the standard diagnosis. Therefore, we hypothesize that this method is more realistic and better suited to human antibiotic concentrations.
Nitro-Oleic Acid-Mediated Nitroalkylation Modulates the Antioxidant Function of Cytosolic Peroxiredoxin Tsa1 during Heat Stress in Saccharomyces cerevisiae
Authors: Aranda-Caño Lorena , Valderrama Raquel , Pedrajas José Rafael , Begara-Morales Juan C. , Chaki Mounira , Padilla María N. , Melguizo Manuel , López-Jaramillo Francisco Javier , Barroso Juan B.
Published: May 2022

Heat stress is one of the abiotic stresses that leads to oxidative stress. To protect themselves, yeast cells activate the antioxidant response, in which cytosolic peroxiredoxin Tsa1 plays an important role in hydrogen peroxide removal. Concomitantly, the activation of the heat shock response (HSR) is also triggered. Nitro-fatty acids are signaling molecules generated by the interaction of reactive nitrogen species with unsaturated fatty acids. These molecules have been detected in animals and plants. They exert their signaling function mainly through a post-translational modification called nitroalkylation. In addition, these molecules are closely related to the induction of the HSR. In this work, the endogenous presence of nitro-oleic acid (NO2-OA) in Saccharomyces cerevisiae is identified for the first time by LC-MS/MS. Both hydrogen peroxide levels and Tsa1 activity increased after heat stress with no change in protein content. The nitroalkylation of recombinant Tsa1 with NO2-OA was also observed. It is important to point out that cysteine 47 (peroxidatic) and cysteine 171 (resolving) are the main residues responsible for protein activity. Moreover, the in vivo nitroalkylation of Tsa1 peroxidatic cysteine disappeared during heat stress as the hydrogen peroxide generated in this situation caused the rupture of the NO2-OA binding to the protein and, thus, restored Tsa1 activity. Finally, the amino acid targets susceptible to nitroalkylation and the modulatory effect of this PTM on the enzymatic activity of Tsa1 are also shown in vitro and in vivo. This mechanism of response was faster than that involving the induction of genes and the synthesis of new proteins and could be considered as a key element in the fine-tuning regulation of defence mechanisms against oxidative stress in yeast.
A Method Based on Multi-Network Feature Fusion and Random Forest for Foreign Objects Detection on Transmission Lines
Authors: Yu Yanzhen , Qiu Zhibin , Liao Haoshuang , Wei Zixiang , Zhu Xuan , Zhou Zhibiao
Published: May 2022

Foreign objects such as kites, nests and balloons, etc., suspended on transmission lines may shorten the insulation distance and cause short-circuits between phases. A detection method for foreign objects on transmission lines is proposed, which combines multi-network feature fusion and random forest. Firstly, the foreign object image dataset of balloons, kites, nests and plastic was established. Then, the Otus binarization threshold segmentation and morphology processing were applied to extract the target region of the foreign object. The features of the target region were extracted by five types of convolutional neural networks (CNN): GoogLeNet, DenseNet-201, EfficientNet-B0, ResNet-101, AlexNet and then fused by concatenation fusion strategy. Furthermore, the fused features in different schemes were used to train and test random forest, meanwhile, the gradient-weighted class activation mapping (Grad-CAM) was used to visualize the decision region of each network, which can verify the effectiveness of the optimal feature fusion scheme. Simulation results indicate that the detection accuracy of the proposed method can reach 95.88%, whose performance is better than the model of a single network. This study provides references for detection of foreign objects suspended on transmission lines.
Dual Auto-Encoder GAN-Based Anomaly Detection for Industrial Control System
Authors: Chen Lei , Li Yuan , Deng Xingye , Liu Zhaohua , Lv Mingyang , Zhang Hongqiang
Published: May 2022

As a core tool, anomaly detection based on a generative adversarial network (GAN) is showing its powerful potential in protecting the safe and stable operation of industrial control systems (ICS) under the Internet of Things (IoT). However, due to the long-tailed distribution of operating data in ICS, existing GAN-based anomaly detection models are prone to misjudging an unseen marginal sample as an outlier. Moreover, it is difficult to collect abnormal samples from ICS. To solve these challenges, a dual auto-encoder GAN-based anomaly detection model is proposed for the industrial control system, simply called the DAGAN model, to achieve an accurate and efficient anomaly detection without any abnormal sample. First, an “encoder–decoder–encoder” architecture is used to build a dual GAN model for learning the latent data distribution without any anomalous sample. Then, a parameter-free dynamic strategy is proposed to robustly and accurately learn the marginal distribution of the training data through dynamic interaction between two GANs. Finally, based on the learned normal distribution and marginal distribution, an optimized anomaly score is used to measure whether a sample is an outlier, thereby reducing the probability of a marginal sample being misjudged. Extensive experiments on multiple datasets demonstrate the advantages of our DAGAN model.
The Inversion Analysis and Material Parameter Optimization of a High Earth-Rockfill Dam during Construction Periods
Authors: Pan Shiyang , Li Tongchun , Shi Guicai , Cui Zhen , Zhang Hanjing , Yuan Li
Published: May 2022

Inversion analysis is usually an efficient solution to process the monitoring data of earth-rockfill dams. However, it is still difficult to obtain calculation results that are consistent with monitoring data due to different construction statuses. To deal with this situation and to introduce a new solution to improve calculation accuracy, the general method of inversion analysis based on back-propagation neural networks and the original step-by-step inversion method assuming that the parameters of the constitutive model vary with construction periods are introduced and verified in this work. Then, both methods are applied in the inversion analysis of a high gravelly soil core rock-fill dam during construction periods. Moreover, the relationship between the inversed material parameters and the stress values of the core wall is discussed. The material parameters are further optimized to obtain more accurate displacement values. The results show that the step-by-step inversion method has a higher accuracy in vertical compression values compared with the conventional inversion method, the trend of material parameter K is more significant than other parameters, and the proposed variable parameter constitutive model has an accuracy between the step-by-step and conventional inversion methods. Conclusions can be drawn that the original step-by-step inversion method has more advantages than the conventional method and the variable parameter constitutive model proposed in this paper might be more suitable for the analysis of a high earth-rockfill dam during construction periods.
Bioactive and Physicochemical Properties of Exotic Fruit Seed Powders: Mango (Mangefiera indica L.) and Rambutan (Nephelium lappaceum L.) Obtained by Various Drying Methods
Authors: Siol Marta , Sadowska Anna , Król Katarzyna , Najman Katarzyna
Published: May 2022

Exotic fruits, which are becoming more and more popular in European countries, contain seeds, which are an unused and useless byproduct of fruit processing. Research conducted in recent years suggests that these unused waste products can be a source of nutrients and bioactive compounds in much more concentrated amounts than those found in the flesh of the fruit. Research on the physicochemical properties and the content of bioactive compounds in fruit seeds may allow the assessment of the possibility and purposefulness of their wider application in the production of functional food. Therefore, the aim of this study was to determine the physicochemical and bioactive properties of exotic, tropical fruit seed powders, such as mango (Mangefiera indica L.) and rambutan (Nephelium lappaceum L.) seeds, obtained by convective drying (CD) and sublimation drying (FD). In the tested powders, the water-holding capacity and water solubility were determined, the color was measured using the ‘electronic eye’ instrumental method, the taste profile was determined using the ‘electronic tongue’, and the content of selected bioactive compounds—such as tannins (titration method), total polyphenols and antioxidant activity was also determined using the spectrophotometric method. It was found that the studied powders were characterized by low water-holding capacity (1.2–1.6 g/1 g of powder), low solubility in water (9.5–17.4%), neutral color and varied taste profile, depending on the origin of the tested powder. Rambutan seed powders were characterized by a more bitter taste with a higher umami-flavor intensity compared to mango seed powders, which showed a more intense acidic and sweet taste. The conducted research shows that the applied methods of powder production, i.e., drying (to similar aw values) by convection vs. sublimation, had a much greater impact on changes in the content of bioactive compounds than on the tested physicochemical parameters. The freeze-dried seed powders were characterized by a higher content of polyphenolic compounds and a higher antioxidant activity than convection-dried seed powders. Considering the high content of polyphenols and high antioxidant activity, the studied powders may find applications in the production of dietary supplements and in the design of functional foods. Due to the low water solubility index, mango and rambutan powders can be used in the design of products where particle sensitivity is indicated. The use of the investigated exotic fruit seed powders, i.e., mango and rambutan, may not only be beneficial for nutritional reasons, but also may contribute to the reduction of post-production waste, in line with the recently widespread “zero waste” trend.
Key Molecules of Fatty Acid Metabolism in Gastric Cancer
Authors: Li Chunlei , Zhang Lilong , Qiu Zhendong , Deng Wenhong , Wang Weixing
Published: May 2022

Fatty acid metabolism is closely linked to the progression of gastric cancer (GC), a very aggressive and life-threatening tumor. This study examines linked molecules, such as Sterol Regulatory Element-Binding Protein 1 (SREBP1), ATP Citrate Lyase (ACLY), Acetyl-CoA Synthases (ACSs), Acetyl-CoA Carboxylase (ACC), Fatty Acid Synthase (FASN), Stearoyl-CoA Desaturase 1 (SCD1), CD36, Fatty Acid Binding Proteins (FABPs), and Carnitine palmitoyltransferase 1 (CPT1), as well as their latest studies and findings in gastric cancer to unveil its core mechanism. The major enzymes of fatty acid de novo synthesis are ACLY, ACSs, ACC, FASN, and SCD1, while SREBP1 is the upstream molecule of fatty acid anabolism. Fatty acid absorption is mediated by CD36 and FABPs, and fatty acid catabolism is mediated by CPT1. If at all possible, we will discover novel links between fatty acid metabolism and a prospective gastric cancer target.
Brain Trauma, Glucocorticoids and Neuroinflammation: Dangerous Liaisons for the Hippocampus
Authors: Komoltsev Ilia G. , Gulyaeva Natalia V.
Published: May 2022

Glucocorticoid-dependent mechanisms of inflammation-mediated distant hippocampal damage are discussed with a focus on the consequences of traumatic brain injury. The effects of glucocorticoids on specific neuronal populations in the hippocampus depend on their concentration, duration of exposure and cell type. Previous stress and elevated level of glucocorticoids prior to pro-inflammatory impact, as well as long-term though moderate elevation of glucocorticoids, may inflate pro-inflammatory effects. Glucocorticoid-mediated long-lasting neuronal circuit changes in the hippocampus after brain trauma are involved in late post-traumatic pathology development, such as epilepsy, depression and cognitive impairment. Complex and diverse actions of the hypothalamic–pituitary–adrenal axis on neuroinflammation may be essential for late post-traumatic pathology. These mechanisms are applicable to remote hippocampal damage occurring after other types of focal brain damage (stroke, epilepsy) or central nervous system diseases without obvious focal injury. Thus, the liaisons of excessive glucocorticoids/dysfunctional hypothalamic–pituitary–adrenal axis with neuroinflammation, dangerous to the hippocampus, may be crucial to distant hippocampal damage in many brain diseases. Taking into account that the hippocampus controls both the cognitive functions and the emotional state, further research on potential links between glucocorticoid signaling and inflammatory processes in the brain and respective mechanisms is vital.
Lymph Nodes as Anti-Tumor Immunotherapeutic Tools: Intranodal-Tumor-Specific Antigen-Pulsed Dendritic Cell Vaccine Immunotherapy
Authors: Morisaki Takashi , Morisaki Takafumi , Kubo Makoto , Morisaki Shinji , Nakamura Yusuke , Onishi Hideya
Published: May 2022

Hundreds of lymph nodes (LNs) are scattered throughout the body. Although each LN is small, it represents a complete immune organ that contains almost all types of immunocompetent and stromal cells functioning as scaffolds. In this review, we highlight the importance of LNs in cancer immunotherapy. First, we review recent reports on structural and functional properties of LNs as sites for antitumor immunity and discuss their therapeutic utility in tumor immunotherapy. Second, we discuss the rationale and background of ultrasound (US)-guided intranodal injection methods. In addition, we review intranodal administration therapy of tumor-specific-antigen-pulsed matured dendritic cells (DCs), including neoantigen-pulsed vaccines.
A Systematic Review of the Guidelines on Venous Thromboembolism Prophylaxis in Gynecologic Oncology
Authors: Romano Federico , Di Lorenzo Giovanni , Stabile Guglielmo , Mirandola Mariateresa , Restaino Stefano , Ianniello Patrizia , Mirenda Giuseppe , Ricci Giuseppe
Published: May 2022

(1) Background: This review aimed to summarize the indications for venous thromboembolic (VTE) events’ prophylaxis in a gynecological cancer population, according to the most recent guidelines. (2) Methods: A systematic review of the guidelines in PubMed, SCOPUS, Web of Science, EMBASE, and CINHAL regarding VTE prevention in gynecological cancer patients was conducted according to PRISMA criteria. We compared the recommendations given by oncological and hematological societies regarding VTE prevention in gynecological cancer patients published from January 2010 through March 2021. We searched for the following keywords: “venous thromboembolism prevention”, “cancer”, and “guidelines”. The AGREE II checklist was used to critically analyze the guidelines’ quality. (3) Results: There were 1003 documents available; 14 met the inclusion criteria, 5 were excluded and, eventually, the guidelines of 10 societies were evaluated. (4) Conclusions: The guidelines agree that low-molecular-weight heparin (LMWH) and fondaparinux achieve better results in VTE prevention in gynecological cancer patients. Direct oral anticoagulants (DOACs) can be used to prevent VTE in outpatients and high-risk medical patients after discharge. VTE risk scores should be applied to all oncological patients to identify those who would benefit from a prevention program. More attention should be paid to mechanical prophylactic methods due to the high bleeding risk of gynecological cancer patients.
Early Diagnosis of Pseudohypoparathyroidism before the Development of Hypocalcemia in a Young Infant
Authors: Hwang Su Kyeong , Shim Ye Jee , Oh Seung Hwan , Jang Kyung Mi
Published: May 2022

Pseudohypoparathyroidism (PHP) is a rare, heterogeneous disorder characterized by end-organ resistance to parathyroid hormone (PTH). PTH resistance causes elevated PTH levels, hypocalcemia, and hyperphosphatemia. Since hypocalcemia causes life-threatening events, early diagnosis is crucial. However, the diagnosis of PHP is elusive during infancy because PHP is usually diagnosed with hypocalcemia-induced symptoms, which develop later in childhood when calcium requirements increase. A 1-month-old girl was referred to our clinic for elevated thyroid-stimulating hormone (TSH) levels on newborn screening. When measured 1 month after levothyroxine treatment, her TSH level normalized. At 4-months-old, multiple hard nodules were noted on her trunk. A punch skin biopsy revealed osteoma cutis associated with Albright’s hereditary osteodystrophy, a major characteristic of PHP. We performed targeted sanger sequencing of the GNAS gene and detected a heterozygous variant c.150dupA (p.Ser51Ilefs*3) in both the proband and her mother, causing frameshift and premature termination mutations. The patient was diagnosed with PHP Ia when she had normal calcium, phosphorous, and PTH levels. We report the early diagnosis of PHP Ia without hypocalcemia. It emphasizes the importance of meticulous physical examination in patients with congenital hypothyroidism.
Managing Sustainability and Resilience of the Built Environment in Developing Communities
Authors: Iskandar Mariham , Nelson Denise , Tehrani Fariborz M.
Published: May 2022

Sustainable built environment has been the primary focus in academic and industrial fields in recent years. The major forces behind sustainable engineering are the rise in climate-related disasters, constant challenges in the energy sector, and a substantial shift in consumers’ consciousness toward conserving natural resources. Further, many professional bodies have developed guidelines and specifications to implement sustainable practices and rate their impacts. Regardless, promoting analytical procedures for creating a context-sensitive design requires professionals to become familiar with standard sustainable practices and feel comfortable implementing more innovative materials and techniques in civil engineering design. In addition, the socio-political environment and macro-economic culture interact with engineering decisions. Hence, these considerations are necessary to deploy these elements in developing communities through best management practices during the lifecycle of sustainable and resilient projects. This paper endeavors to review these practices using lessons learned from applied examples and existing literature. Discussions cover various aspects of project development, from planning to demolition. Recommendations address challenges and opportunities in the sustainable development of resilient built infrastructure in developing regions.
Gene Transfer Agent g5 Gene Reveals Bipolar and Endemic Distribution of Roseobacter Clade Members in Polar Coastal Seawater
Authors: Zeng Yin-Xin , Li Hui-Rong , Luo Wei
Published: May 2022

The Roseobacter clade represents one of the most abundant groups of marine bacteria and plays important biogeochemical roles in marine environments. Roseobacter genomes commonly contain a conserved gene transfer agent (GTA) gene cluster. A major capsid protein-encoding GTA (g5) has been used as a genetic marker to estimate the diversity of marine roseobacters. Here, the diversity of roseobacters in the coastal seawater of Arctic Kongsfjorden and Antarctic Maxwell Bay was investigated based on g5 gene clone library analysis. Four g5 gene clone libraries were constructed from microbial assemblages representing Arctic and Antarctic regions. The genus Phaeobacter was exclusively detected in Arctic seawater, whereas the genera Jannaschia, Litoreibacter and Pacificibacter were only observed in Antarctic seawater. More diverse genera within the Roseobacter clade were observed in Antarctic clones than in Arctic clones. The genera Sulfitobacter, Loktanella and Yoonia were dominant (higher than 10% of total clones) in both Arctic and Antarctic samples, implying their roles in polar marine environments. The results not only indicated a bipolar or even global distribution of roseobacters in marine environments but also showed their endemic distribution either in the Arctic or Antarctic. Endemic phylotypes were more frequently observed in polar regions than cosmopolitan phylotypes. In addition, endemic phylotypes were more abundant in Arctic samples (84.8% of Arctic sequences) than in Antarctic samples (54.3% of Antarctic sequences).
Deep Convolutional Recurrent Model for Automatic Scoring Sleep Stages Based on Single-Lead ECG Signal
Authors: Urtnasan Erdenebayar , Park Jong-Uk , Joo Eun Yeon , Lee Kyoung-Joung
Published: May 2022

Background: Sleep stage scoring, which is an essential step in the quantitative analysis of sleep monitoring, relies on human experts and is therefore subjective and time-consuming; thus, an easy and accurate method is needed for the automatic scoring of sleep stages. Methods: In this study, we constructed a deep convolutional recurrent (DCR) model for the automatic scoring of sleep stages based on a raw single-lead electrocardiogram (ECG). The DCR model uses deep convolutional and recurrent neural networks to apply the complex and cyclic rhythms of human sleep. It consists of three convolutional and two recurrent layers and is optimized by dropout and batch normalization. The constructed DCR model was evaluated using multiclass classification, including five-class sleep stages (wake, N1, N2, N3, and rapid eye movement (REM)) and three-class sleep stages (wake, non-REM (NREM), and REM), using a raw single-lead ECG signal. The single-lead ECG signal was collected from 112 subjects in two groups: control (52 subjects) and sleep apnea (60 subjects). The single-lead ECG signal was preprocessed, segmented at a duration of 30 s, and divided into a training set of 89 subjects and test set of 23 subjects. Results: We achieved an overall accuracy of 74.2% for five classes and 86.4% for three classes. Conclusions: These results show the DCR model’s superior performance over those in the previous studies, highlighting that the model can be an alternative tool for sleep monitoring and sleep screening.
The Promise of Multicancer Early Detection. Comment on Pons-Belda et al. Can Circulating Tumor DNA Support a Successful Screening Test for Early Cancer Detection? The Grail Paradigm. Diagnostics 2021, 11, 2171
Authors: Klein Eric A. , Beer Tomasz M. , Seiden Michael
Published: May 2022

Multicancer Early Detection (MCED) represents a new and exciting paradigm for the early detection of cancer, which is the leading cause of death worldwide. Current screening tests, recommended for only five cancer types (breast, lung, colon, cervical, and prostate), are limited by a lack of complete adherence to guideline-based use and by the fact that they have cumulative high false positive rates. MCED tests agnostically detect cancer signals in the blood with good sensitivity and low false positive rates, can predict the cancer site of origin with high accuracy, can detect highly lethal cancers that have no current screening tests, and promise to improve cancer screening by improving efficiency and reducing the overall number needed to screen. Herein we outline this promise and clarify several published misconceptions about this field.
High-Speed Reservoir Computing Based on Circular-Side Hexagonal Resonator Microlaser with Optical Feedback
Authors: Zhao Tong , Xie Wenli , Guo Yanqiang , Xu Junwei , Guo Yuanyuan , Wang Longsheng
Published: May 2022

In the current environment of the explosive growth in the amount of information, the demand for efficient information-processing methods has become increasingly urgent. We propose and numerically investigate a delay-based high-speed reservoir computing (RC) using a circular-side hexagonal resonator (CSHR) microlaser with optical feedback and injection. In this RC system, a smaller time interval can be obtained between virtual nodes, and a higher information processing rate (Rinf) can also be achieved, due to the ultra-short photon lifetime and wide bandwidth of the CSHR microlaser. The performance of the RC system was tested with three benchmark tasks (Santa-Fe chaotic time series prediction task, the 10th order Nonlinear Auto Regressive Moving Average task and Nonlinear channel equalization task). The results show that the system achieves high-accuracy prediction, even with a small number of virtual nodes (25), and is more feasible, with lower requirements for arbitrary waveform generators at the same rate. Significantly, at the high rate of 10 Gbps, low error predictions can be achieved over a large parameter space (e.g., frequency detuning in the interval 80 GHz, injected strength in the range of 0.9 variation and 2% range for feedback strength). Interestingly, it has the potential to achieve Rinf of 25 Gbps under technical advancements. Additionally, its shorter external cavity length and cubic micron scale size make it an excellent choice for large-scale photonic integration reservoir computing.
Integrated Functional Safety and Cybersecurity Evaluation in a Framework for Business Continuity Management
Authors: Kosmowski Kazimierz T. , Piesik Emilian , Piesik Jan , Śliwiński Marcin
Published: May 2022

This article outlines an integrated functional safety and cybersecurity evaluation approach within a framework for business continuity management (BCM) in energy companies, including those using Industry 4.0 business and technical solutions. In such companies, information and communication technology (ICT), and industrial automation and control system (IACS) play important roles. Using advanced technologies in modern manufacturing systems and process plants can, however, create management impediments due to the openness of these technologies to external systems and networks via various communication channels. This makes company assets and resources potentially vulnerable to risks, e.g., due to cyber-attacks. In the BCM-oriented approach proposed here, both preventive and recovery activities are considered in light of engineering best practices and selected international standards, reports, and domain publications.
Investigating Long-Term Commitments to Replace Electricity Generation with SMRs and Estimates of Climate Change Impact Costs Using a Modified VENSIM Dynamic Integrated Climate Economy (DICE) Model
Authors: Shobeiri Elaheh , Shen Huan , Genco Filippo , Tokuhiro Akira
Published: May 2022

During the last few years, nuclear energy has received great attention due to the increase in climate change awareness. According to the Paris agreement, global temperature is to be kept below 2 °C and preferably below 1.5 °C by 2050. This approach has been substantially confirmed in the recent COP 26 in Glasgow. This research investigates the effects of integrating SMR nuclear power plants (small modular reactors) into the Nordhaus Dynamic Integrated Climate Economy (DICE) model for reducing the CO2 emissions in the atmosphere by substituting all existing fossil-fueled power plants (FPPP). The software is based on the VENSIM dynamic systems modeling platform. Simulations were carried out from the year 2019 to 2100 using 10-year increments. Several scenarios were thus simulated replacing roughly 70,000 FPPPs operating at this time in the world. Simulations indicate a CO2 reduction of approximately 12.63% relative to the initial conditions used and using 87,830 SMR core units of 80 MWe electric each to meet such demand. The DICE model further predicts the cost of climate damage impacting the upper ocean and atmospheric temperatures, and the deep ocean temperature as USD 1.515 trillion (US Dollar; (US) trillion = 1,000,000,000,000 (1 × 1012)) by the end of this century. From a modified section of the model, a cost of USD 1.073 trillion is predicted as the toll on human health costs. This is thus equal to a USD 2.59 trillion loss in the economy.
Multi-Stacked Superbuck Converter-Based Single-Switch Charger Integrating Cell Voltage Equalizer for Series-Connected Energy Storage Cells
Authors: Uno Masatoshi , Xu Qi , Sato Yusuke
Published: May 2022

Voltages of series-connected energy storage cells, such as electric double-layer capacitors (EDLCs) and lithium-ion batteries, need to be equalized to ensure years of safe operation. However, to this end, a voltage equalizer is necessary in addition to a charger, increasing the system complexity and cost. This paper proposes a family of transformerless single-switch integrated chargers that merge a charger and equalizer into a single unit, achieving a simplified system and circuit. Proposed integrated chargers are derived by stacking multiple conventional pulse width modulation (PWM) converters, such as a superbuck converter, that contain two inductors and one energy transfer capacitor. Detailed operation analyses, including an investigation on the impact of component tolerance on voltage equalization performance, are also performed. Experimental charging tests using a 12-W prototype were performed for four EDLC cells. All cells were charged with eliminating voltage imbalance and demonstrating the charging and equalization performance of the proposed integrated charger.
Analysis of Customer Satisfaction with the Quality of Energy Market Services in Poland
Authors: Rosak-Szyrocka Joanna , Żywiołek Justyna , Mrowiec Maciej
Published: May 2022

Environmental protection, sustainable development, quality, and value have become the goals of societal development in the twenty-first century. As the core of environmental protection, the new energy sector has become a widespread trend. In this article, we will look at how well and how satisfied customers are with the energy market’s service. A literature review of energy sector quality and value issues was conducted. The survey was conducted in 2021 in Poland with over 2404 respondents by the CAWI survey. Additionally, it was given a qualitative analysis. In the next step, we applied selected quality management methods and tools. The results of the study clearly show that the respondents expect energy companies to become more involved in building customer–enterprise relationships. Research shows that employees of energy companies should be trained more often in the field of customer service, especially in terms of an individual approach to the customer, which has value for the customer. The authors propose ways to build an arrow diagram to increase customer satisfaction levels. A model of elements determining quality as well as value for clients in the energy market was built.
Modified Biochars and Their Effects on Soil Quality: A Review
Authors: Hafeez Abdul , Pan Taowen , Tian Jihui , Cai Kunzheng
Published: May 2022

Biochar (BC) has attracted attention due to its impacts on soil quality by enhancing soil fertility, carbon storage and contaminants immobilization. BC also induces changes in microbial community structure and enhances crop productivity in long term scenarios compared to many other organic amendments. However, information related to the role of modified BCs in altering the soil quality is still scarce. BC can be modified by using physical, chemical and microbial methods. Modified BC can change the functional groups, pore size, pore structure, surface area and chemical properties of soil, which plays a key role in changing the soil quality. The addition of modified BCs as soil amendment increased soil CEC (cation exchange capacity), EC (electron conductivity), pH, organic matter, hydraulic conductivity, soil porosity, infiltration rate, microbial activities (enzymes and community), nutrient profile and gas exchange properties, but it varies according to the soil structure and pervading environmental conditions. This study provides a basis for effective practical approaches to modifying BCs for improving soil quality.
Thermal Processing of Liquid Egg Yolks Modulates Physio-Chemical Properties of Mayonnaise
Authors: Ho Jou-Hsuan , Lee Tan-Ang , Namai Nobuaki , Sakai Shunji , Lou Siao-Syuan , Handa Akihiro , Lin Wan-Teng
Published: May 2022

In this study, the effect of various heating temperatures (61–70 °C) and times (1–10 min) on physical and chemical properties of liquid egg yolk (LEY) and mayonnaise were investigated. Initially, we found that the increase of LEY protein denaturation was highly correlated with the increase of temperature and time, without causing either protein degradation or aggregation. In addition, the viscosity and particle size of LEY were significantly increased with greater heating temperature and time. Furthermore, the emulsification stability of mayonnaise prepared from thermally processed LEY were significantly better than that of the unheated control group, in particular, the emulsion stability of mayonnaise was higher at a temperature ranging from 62 °C to 68 °C, whereas the emulsion stability decreased above 69 °C. A rheological analysis showed that mayonnaise prepared from thermally processed LEY has higher shear stress when compared with the control group. Indeed, a sharp increase in the shear stress was observed when LEY was heated above 67 °C. Results from storage behavior analysis suggest that mayonnaise prepared from thermally processed LEY failed to affect the chemical qualities of mayonnaise, as evidenced by the fact that acid values and TBA values were not statistically significant with the unheated control group. Microscopic observation indicates that the number of complete oil droplets were significantly reduced at higher heating (70 °C/5 and 10 min) conditions. Finally, the sensory evaluation results suggest that mayonnaise prepared from thermally processed LEY does not influence the appearance, aroma, taste, greasy feeling, and overall acceptance of mayonnaise, as indicated by there being no significant differences between the experimental group and the control group (p > 0.05). We conclude from our study that a combination of heating conditions over 67 °C/5 min can allow the mayonnaise to retain better quality in terms of stability.
Left Riemann–Liouville Fractional Sobolev Space on Time Scales and Its Application to a Fractional Boundary Value Problem on Time Scales
Authors: Hu Xing , Li Yongkun
Published: May 2022

First, we show the equivalence of two definitions of the left Riemann–Liouville fractional integral on time scales. Then, we establish and characterize fractional Sobolev space with the help of the notion of left Riemann–Liouville fractional derivative on time scales. At the same time, we define weak left fractional derivatives and demonstrate that they coincide with the left Riemann–Liouville ones on time scales. Next, we prove the equivalence of two kinds of norms in the introduced space and derive its completeness, reflexivity, separability, and some embedding. Finally, as an application, by constructing an appropriate variational setting, using the mountain pass theorem and the genus properties, the existence of weak solutions for a class of Kirchhoff-type fractional p-Laplacian systems on time scales with boundary conditions is studied, and three results of the existence of weak solutions for this problem is obtained.
Modified-Dose Pembrolizumab and Prognostic Outcomes among Non-Small Cell Lung Cancer Patients: A Chart Review Study
Authors: To Sheng-Yin , Kao Li-Ting , Shih Jui-Hu , Li I-Hsun , Huang Tsai-Wang , Tsai Chen-Liang , Chian Chih-Feng , Ho Ching-Liang , Chang Ping-Ying
Published: May 2022

The modified dose (MD) regimen of pembrolizumab (2 mg/kg or 100 mg every 3 weeks) is an alternative option to reduce the financial burden resulting from the extremely high cost of the standard dose (SD) regimen (200 mg every 3 weeks). However, the clinical effectiveness and prognostic outcomes have not been fully elucidated in real-word clinical practice. Sixty-four consecutive patients in Taiwan receiving pembrolizumab for advanced NSCLC between 2018 and 2020 were recruited in this study. Comparisons of overall survival (OS) and progression-free survival (PFS) were performed using Kaplan–Meier survival curves. Additionally, 12 predictors, including pembrolizumab regimen, dose, neutrophil-to-lymphocyte ratio (NLR), age, sex, histopathology, smoking history, ECOG PS, EGFR mutation, PD-L1 expression, distant metastases and treatment line, were analyzed in multivariable Cox models for predicting OS and PFS. The results showed that the MD group and the SD group had similar OS and PFS, especially in patients beyond first-line treatment or with a pretreatment NLR < 5. The NLR was the only independent factor associated with both OS (adjusted HR = 0.052; p = 0.010) and PFS (adjusted HR = 0.259; p = 0.021). The results of this study assure the clinical effectiveness of MD pembrolizumab and suggest that the pretreatment NLR could highlight patients who may benefit from MD pembrolizumab.
The Arsenic–Antimony Creek at Sauerbrunn/Burgenland, Austria: A Toxic Habitat for Amphibians
Authors: Adlassnig Wolfram , Schmidt Brigitte , Jirsa Franz , Gradwohl Andreas , Ivesic Caroline , Koller-Peroutka Marianne
Published: May 2022

(1) Background: All Austrian amphibians are affected by the degradation of habitats. Mining contributes to habitat destruction by the formation of spoil heaps and mine drainage waters. In Stadtschlaining/Burgenland, antimony mining led to increased arsenic (As) and antimony (Sb) concentrations in soil and water. This study investigates a contaminated creek, still inhabited by amphibians. (2) Methods: Water and soil were analyzed along the creek and correlated with the occurrence of amphibians. (3) Results: As and Sb were increased, with up to 49,000 mg/kg As and 2446 mg/kg Sb in the soil. Up to 317 mg/kg As and 156 mg/kg Sb became bioavailable under gastric, and up to 298 mg/kg As and 30 mg/kg Sb under intestinal conditions, and were absorbed upon ingestion of soil. Larvae of Salamandra salamandra were found throughout the creek; survival rates were low. Rana temporaria occurs in the most contaminated sections but does not propagate here. Bombina variegata appears occasionally. Amphibians seem not to be able to detect and avoid metal or metalloid contamination. (4) Conclusion: Survival of larvae is dubious, but adult amphibians survive without apparent damage under severe metalloid contamination.
Social Support and Drug Abstention Motivation among Chinese Male Drug Addicts: A Moderated Mediation Model of Self-Control and Sensation-Seeking
Authors: Xu Xizheng , Wu Yunpeng , Zhou Senlin
Published: May 2022

The goal of this study is to examine how social support affects Chinese male drug addicts’ abstention motivation. To elucidate the mechanism as well as the boundary condition of the aforesaid influence, self-control and sensation-seeking were induced. Using the questionnaire method, the cross-sectional data were collected from 498 male drug addicts from one hospital and four compulsory isolation drug abstention centers in Central China region. The results indicated that social support has a positive direct and indirect effect on abstention motivation. The indirect influence is that the impact of social support on abstention motivation is mediated by self-control. The direct effect was moderated by sensation-seeking. Specifically, for individuals with low sensation-seeking, social support can significantly increase drug abstinence motivation, but this effect was not significant for those with high sensation-seeking. Theoretical and practical implications of the results were discussed.
Tie-Over Bolster Pressure Dressing Improves Outcomes of Skin Substitutes Xenografts on Athymic Mice
Authors: Cartier Andréanne , Barbier Martin A. , Larouche Danielle , Morissette Amélie , Bussières Ariane , Montalin Livia , Beaudoin Cloutier Chanel , Germain Lucie
Published: May 2022

The efficacy of skin substitutes is established for the treatment of burn injuries, but its use is not limited to this condition. This technology has the potential to improve the treatment of various conditions by offering highly advanced and personalized treatments. In vivo studies are challenging but essential to move to clinical use in humans. Mice are the most widely used species in preclinical studies, but the main drawback of this model is the limited surface area of the graft in long-term transplantation studies caused by the displacement and the contraction of the graft. We improved the conventional surgical procedures by stabilizing the chamber covering the graft with intramuscular sutures and by adding a tie-over bolster dressing. The current study was therefore performed to compare outcomes of skin grafts between the conventional and optimized skin graft model. Human self-assembled skin substitutes (SASSs) were prepared and grafted to athymic mice either by the conventional method or by the new grafting method. Graft healing and complications were assessed using digital photographs on postoperative days 7, 14, and 21. Similar structure and organization were observed by histological staining. The new grafting method reduced medium and large displacement events by 1.26-fold and medium and large contraction events by 1.8-fold, leading to a 1.6-fold increase in graft surface area compared to skin substitutes grafted with the usual method. This innovation ensures better reproducibility and consistency of skin substitute transplants on mice.
Tyrosine Kinase Src Is a Regulatory Factor of Bone Homeostasis
Authors: Matsubara Takuma , Yasuda Kazuma , Mizuta Kana , Kawaue Hiroka , Kokabu Shoichiro
Published: May 2022

Osteoclasts, which resorb the bone, and osteoblasts, which form the bone, are the key cells regulating bone homeostasis. Osteoporosis and other metabolic bone diseases occur when osteoclast-mediated bone resorption is increased and bone formation by osteoblasts is decreased. Analyses of tyrosine kinase Src-knockout mice revealed that Src is essential for bone resorption by osteoclasts and suppresses bone formation by osteoblasts. Src-knockout mice exhibit osteopetrosis. Therefore, Src is a potential target for osteoporosis therapy. However, Src is ubiquitously expressed in many tissues and is involved in various biological processes, such as cell proliferation, growth, and migration. Thus, it is challenging to develop effective osteoporosis therapies targeting Src. To solve this problem, it is necessary to understand the molecular mechanism of Src function in the bone. Src expression and catalytic activity are maintained at high levels in osteoclasts. The high activity of Src is essential for the attachment of osteoclasts to the bone matrix and to resorb the bone by regulating actin-related molecules. Src also inhibits the activity of Runx2, a master regulator of osteoblast differentiation, suppressing bone formation in osteoblasts. In this paper, we introduce the molecular mechanisms of Src in osteoclasts and osteoblasts to explore its potential for bone metabolic disease therapy.
GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production
Authors: Li Xue , Fu Qi , Zhao Fu-Xing , Wu Yi-Qing , Zhang Teng-Yue , Li Zhong-Qi , He Jun-Min
Published: May 2022

Heterotrimeric G proteins function as key players in guard cell signaling to many stimuli, including ultraviolet B (UV-B) and ethylene, but whether guard cell G protein signaling is activated by the only one potential G protein-coupled receptor, GCR1, is still unclear. Here, we found that gcr1 null mutants showed defects in UV-B- and ethylene-induced stomatal closure and production of reactive oxygen species (ROS) and nitric oxide (NO) in guard cells, but these defects could be rescued by the application of a Gα activator or overexpression of a constitutively active form of Gα subunit GPA1 (cGPA1). Moreover, the exogenous application of hydrogen peroxide (H2O2) or NO triggered stomatal closure in gcr1 mutants and cGPA1 transgenic plants in the absence or presence of UV-B or ethylene, but exogenous ethylene could not rescue the defect of gcr1 mutants in UV-B-induced stomatal closure, and gcr1 mutants did not affect UV-B-induced ethylene production in Arabidopsis leaves. These results indicate that GCR1 positively controls UV-B- and ethylene-induced stomatal closure by activating GPA1-dependent ROS and NO production in guard cells and that ethylene acts upstream of GCR1 to transduce UV-B guard cell signaling, which establishes the existence of a classic paradigm of G protein signaling in guard cell signaling to UV-B and ethylene.