bluuebunny/crossref_metadata_2025 huggingface dataset:
Atmospheric drivers affect crop yields in Mozambique
Authors: Takele Robel , Buizza Roberto , Dell'Acqua Matteo
Published: September 2023

Climate change has been inducing variations in the statistics of both the large-scale weather patterns and the local weather in many regions of the world, and these variations have been affecting several human activities, including agriculture. In this study, we look at the links between large-scale weather patterns and local weather as well as agriculture, with a specific regional focus on Mozambique between 1981 and 2019. First, we investigated linear trends and links between large-scale weather patterns and local weather in the region using the ERA5 dataset. We used the same data to investigate how climate change has been affecting the statistics of large-scale weather patterns. Then, we derived Mozambique country-level cereal yield data from FAO and linked it up with climate and weather data to assess what is the relationship between large-scale patterns and local agronomic outputs using a multiple linear regression (MLR) model with crop yield as the response variable and climate drivers as predictors. The results indicate that in Mozambique, the crop season warmed substantially and consistently with climate change-induced global warming, and the rainy season had become drier and shorter, with precipitation concentrated in fewer, more intense events. These changes in the local weather have been linked to variations in the statistics of large-scale weather patterns that characterize the (large-scale) atmospheric flow over the region. Our results indicate a negative impact on yield associated with climate change, with average yield losses of 20% for rice and 8% for maize over the analyzed period (1981–2019). This negative impact suggests that, at the country scale, further future warming during the growing season may offset some of the cereal yield gains from technological advances.
Examining the U-shaped relationship of sleep duration and systolic blood pressure with risk of cardiovascular events using a novel recursive gradient scanning model
Authors: Yang Shuo , Zhang Nanxiang , Liang Zichao , Han Yuduan , Luo Hao , Ge Yingfeng , Yin Jianan , Ding Chonglong , Li Chao , Zhang Qitong , Zhang Jinxin
Published: September 2023

Background

Observational studies have suggested U-shaped relationships between sleep duration and systolic blood pressure (SBP) with risks of many cardiovascular diseases (CVDs), but the cut-points that separate high-risk and low-risk groups have not been confirmed. We aimed to examine the U-shaped relationships between sleep duration, SBP, and risks of CVDs and confirm the optimal cut-points for sleep duration and SBP.

Methods

A retrospective analysis was conducted on NHANES 2007–2016 data, which included a nationally representative sample of participants. The maximum equal-odds ratio (OR) method was implemented to obtain optimal cut-points for each continuous independent variable. Then, a novel “recursive gradient scanning method” was introduced for discretizing multiple non-monotonic U-shaped independent variables. Finally, a multivariable logistic regression model was constructed to predict critical risk factors associated with CVDs after adjusting for potential confounders.

Results

A total of 26,691 participants (48.66% were male) were eligible for the current study with an average age of 49.43 ± 17.69 years. After adjusting for covariates, compared with an intermediate range of sleep duration (6.5–8.0 h per day) and SBP (95–120 mmHg), upper or lower values were associated with a higher risk of CVDs [adjusted OR (95% confidence interval) was 1.20 (1.04–1.40) for sleep duration and 1.17 (1.01–1.36) for SBP].

Conclusions

This study indicates U-shaped relationships between SBP, sleep duration, and risks of CVDs. Both short and long duration of sleep/higher and lower BP are predictors of cardiovascular outcomes. Estimated total sleep duration of 6.5–8.0 h per day/SBP of 95–120 mmHg is associated with lower risk of CVDs.
Breaking the barriers: addressing dental students bullying by medical counterparts and promoting a collaborative learning environment
Authors: Qamar Wajiha , Qayum Mehran , Nisar Anita
Published: September 2023

The study question on the bullying of dental students by their medical counterparts is of the utmost significance due to the policy implications and the necessity for additional research. Despite anecdotal evidence showing instances of competitiveness and exploitation between dental and medical students, there is an absence of comprehensive studies on the incidence and impact of bullying specifically targeted against dental students. This research gap is concerning since bullying has a negative impact on people’s mental health, academic success, and professional development. This review provides an in-depth study of the matter, allowing the policymakers better understand the scope and dynamics of bullying among medical and dental students, to enable them develop specialized policies and programs to address the problem.
Case Report: A 65-year-old man with paraganglioma accompanied by elevated interleukin-6 levels and KIF1B single gene mutation
Authors: Wang Chi , Guan Ming , Zhang Shuang , Cui Can
Published: September 2023

Paraganglioma is a less prevalent disease, and paraganglioma with only secreting interleukin-6 (IL-6) has not been previously reported. A 64-year-old male patient came to the hospital with the chief complaints of fever and palpitations. The peak body temperature was 38.7°C (101.66°F). Heart rate was 110 bpm, while blood pressure was in the normal range. Antibiotics and antiviral therapies were ineffective. The levels of blood IL-6, C-reactive protein (CRP), alkaline phosphatase (ALP), platelets (PLT), glutamyltransferase (GGT), fibrinogen, and D-dimer were all elevated. Infectious diseases, auto-immune diseases, and hematological malignancy were all excluded. Nearly 10 years ago, a large retroperitoneal mass of the patient was detected by accident. Fortunately, there have been no special symptoms for the past 10 years after regular follow-up. After admission this time, PET-CT was performed. A large confounding density mass at the upper part of the abdominal and retroperitoneal area was seen, and the possibility of paraganglioma was considered. However, biochemical assays for blood and urine catecholamine and their metabolites including adrenaline, norepinephrine, 3-methoxytyramine, methoxyepinephrine, methoxynorepinephrine, and vanillylmandelic acid were all in normal range in spite of mild elevated dopamine with no significance. The whole-exome capture and sequencing of the genomic DNA of the patient showed a heterozygous mutation in the coding site of KIF1B gene (Coding: NM_015047.3:c.4660G>C, Mutation: p.Val1554Leu; chromosomal location was chr1: 10428570). The mutation at this locus of KIF1B has not been reported previously. The patient refused the surgical treatment. Because the mass burdens several important organs including the pancreas, the risk of surgery was high. Doxazosin was then administered to the patient. After taking doxazosin, the symptoms disappeared rapidly. Body temperature returned to normal range in 3 days. Heart rate decreased to approximately 90 bpm. In the following days, the levels of IL-6, CPR, ALP, platelets, GGT fibrinogen, and D-dimer continued to decrease. After 63 days of taking doxazosin, IL-6 level was completely normal. After 190 days of medication, hemoglobin (Hb) and GGT levels also returned to the normal range. After 1 year onset, the patient again underwent a blood test. Almost all blood indexes were in the normal range including IL-6.
Transitioning to sustainable energy: opportunities, challenges, and the potential of blockchain technology
Authors: Lv Yongjun
Published: September 2023

The pressing issues of climate change and the limited availability of non-renewable energy resources have created a growing need for sustainable energy alternatives. This study provides a comprehensive overview of the pressing need for sustainable energy solutions and the complex relationship between energy and the economy. The challenges and opportunities presented by the transition to sustainable energy sources are explored, including the need for investment in renewable energy technologies, policy changes to incentivize sustainable energy use, and the potential for job creation in the sustainable energy sector. On the other hand, it is recognized that there are considerable hurdles that need to be addressed, including the substantial initial expenses associated with establishing renewable energy systems, as well as the political and societal barriers to enacting change. The economic benefits of transitioning to sustainable energy, such as improved energy security, reduced dependence on fossil fuels, and the potential for increased economic growth, are evaluated. The complex relationship between energy and the economy is thoroughly analyzed, presenting a valuable contribution to the academic literature on sustainable energy. Furthermore, an inquiry is being made into the potential contribution of blockchain technology in advancing a sustainable energy landscape. This includes its ability to augment the effectiveness and openness of energy markets, as well as its capacity to assist in the assimilation of renewable energy resources. Hence, this research underscores the importance of transitioning to sustainable energy sources for their environmental and economic merits. The findings presented offer valuable insights to inform policy decisions and guide future research endeavors in this field. By promoting the advancement of sustainable energy technologies, this study contributes to the development of a more sustainable global economy.
Transmission line bolts and their defects detection method based on position relationship
Authors: Zhao Zhenbing , Xiong Jing , Han Yu , Miao Siyu
Published: September 2023

Introduction: To solve the problems of small proportion of bolts in aerial images of power transmission lines, small differences between classes, and difficulty in extracting refined features, this paper proposes a method for detecting power transmission line bolts and their defects based on positional relationships.

Methods: Firstly, a spatial attention module is added to Faster R-CNN, using two parallel cross attention to obtain cross path features and global features respectively, and spatial feature enhancement is performed on the features output from the convolution layer. Then, starting from the spatial position relationship of bolts and their defects, using the relative geometric features of candidate regions as input, the spatial position relationship of bolts and their defects on the image is modeled. Finally, the position features and regional features are connected to obtain enhanced features. The bolt position knowledge on the connecting plate is added to the detection model to improve the detection accuracy of the model.

Results and discussion: The experimental results show that the mAP value of the algorithm in this paper is increased by 6.61% compared to the Faster R-CNN detection model in aerial photography of transmission line bolts and their defect datasets, with the AP value of normal bolts increased by 1.73%, the AP value of pin losing increased by 4.45%, and the AP value of nut losing increased by 13.63%.
cGAS-STING signaling pathway in intestinal homeostasis and diseases
Authors: Yang Yuchen , Wang Li , Peugnet-González Ivonne , Parada-Venegas Daniela , Dijkstra Gerard , Faber Klaas Nico
Published: September 2023

The intestinal mucosa is constantly exposed to commensal microbes, opportunistic pathogens, toxins, luminal components and other environmental stimuli. The intestinal mucosa consists of multiple differentiated cellular and extracellular components that form a critical barrier, but is also equipped for efficient absorption of nutrients. Combination of genetic susceptibility and environmental factors are known as critical components involved in the pathogenesis of intestinal diseases. The innate immune system plays a critical role in the recognition and elimination of potential threats by detecting pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). This host defense is facilitated by pattern recognition receptors (PRRs), in which the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway has gained attention due to its role in sensing host and foreign double-stranded DNA (dsDNA) as well as cyclic dinucleotides (CDNs) produced by bacteria. Upon binding with dsDNA, cGAS converts ATP and GTP to cyclic GMP-AMP (cGAMP), which binds to STING and activates TANK binding kinase 1 (TBK1) and interferon regulatory factor 3 (IRF3), inducing type I interferon (IFN) and nuclear factor kappa B (NF-κB)-mediated pro-inflammatory cytokines, which have diverse effects on innate and adaptive immune cells and intestinal epithelial cells (IECs). However, opposite perspectives exist regarding the role of the cGAS-STING pathway in different intestinal diseases. Activation of cGAS-STING signaling is associated with worse clinical outcomes in inflammation-associated diseases, while it also plays a critical role in protection against tumorigenesis and certain infections. Therefore, understanding the context-dependent mechanisms of the cGAS-STING pathway in the physiopathology of the intestinal mucosa is crucial for developing therapeutic strategies targeting the cGAS-STING pathway. This review aims to provide insight into recent findings of the protective and detrimental roles of the cGAS-STING pathway in intestinal diseases.
Therapeutic role of interleukin-1 receptor antagonist in pancreatic diseases: mendelian randomization study
Authors: Yuan Shuai , Miao Yuyang , Ruan Xixian , Chen Jie , Li Xue , Larsson Susanna C.
Published: September 2023

Background

The interleukin-1 pathway has been linked to pancreatic diseases. We applied the Mendelian randomization approach to explore whether higher interleukin-1 receptor antagonist (IL-1RA) levels reduce the risk of acute and chronic pancreatitis and pancreatic cancer.

Methods

Genetic variants associated with blood IL-1RA levels at the genome-wide significance level and located 5MB downstream or upstream of the IL1RN gene were extracted from a genome-wide meta-analysis of 21,758 participants. After pruning, genetic variants without linkage disequilibrium were used as genetic instrument for IL-1RA. Summary-level data on acute and chronic pancreatitis and pancreatic cancer were obtained from the UK Biobank and FinnGen studies. The associations were meta-analyzed for one outcome from two sources.

Results

Genetically predicted higher levels of IL-1RA were associated with a lower risk of acute and chronic pancreatitis and pancreatic cancer. In the meta-analysis of UK Biobank and FinnGen, the combined odds ratio was 0.87 (95% confidence interval [CI] 0.77-0.97, P=0.003) for acute pancreatitis, 0.73 (95% CI 0.65-0.82, P=2.93×10-8) for chronic pancreatitis, and 0.86 (95% CI 0.77-0.96, P=0.009) for pancreatic cancer per one standard deviation increment in genetically predicted levels of IL-1RA.

Conclusion

This study suggests a protective role of IL-1RA in three major pancreatic diseases, which hints the therapeutic potentials of IL-1RA in pancreatic diseases.
Association of N-acetylcysteine use with contrast-induced nephropathy: an umbrella review of meta-analyses of randomized clinical trials
Authors: Zhu Rong , Zheng Rong , Deng Bing , Liu Ping , Wang Yiru
Published: September 2023

Background

The effectiveness of N-acetylcysteine (NAC) in treating contrast-induced nephropathy (CIN) has been the subject of conflicting meta-analyses, but the strength of the evidence for these correlations between NAC use and CIN has not been measured overall.

Objective

To evaluate the data from randomized clinical studies (RCTs) that examined the relationships between NAC use and CIN in meta-analyses.

Methods

Between the creation of the database and April 2023, searches were made in PubMed, Cochrane Library, EMBASE, and Web of Science. N-acetylcysteine, contrast-induced nephropathy, or contrast-induced renal disease were among the search keywords used, along with terms including systematic review and meta-analysis. The Assessment of Multiple Systematic Reviews, version 2, which assigned grades of extremely low, low, moderate, or high quality to each meta-analysis’s scientific quality, was used to evaluate each meta-analysis. The confidence of the evidence in meta-analyses of RCTs was evaluated using the Grading of Recommendation, Assessment, Development and Evaluations method, with evidence being rated as very low, low, moderate, or high.

Results

In total, 493 records were screened; of those, 46 full-text articles were assessed for eligibility, and 12 articles were selected for evidence synthesis as a result of the screening process. Based on the pooled data, which was graded as moderate-quality evidence, it can be concluded that NAC can decrease CIN (OR 0.72, 95% CI 0.65–0.79, p < 0.00001) and blood levels of serum creatinine (MD −0.09, 95% CI −0.17 to −0.01, p = 0.03). In spite of this, there were no associations between NAC and dialysis requirement or mortality in these studies.

Conclusion

The results of this umbrella review supported that the renal results were enhanced by NAC. The association was supported by moderate-quality evidence.

Systematic review registration

[https://clinicaltrials.gov/], identifier [CRD42022367811].
Isolation, characterization, and genomic analysis of a novel bacteriophage MA9V-1 infecting Chryseobacterium indologenes: a pathogen of Panax notoginseng root rot
Authors: Zou He , Ding Yafang , Shang Junjie , Ma Chunlan , Li Jinhua , Yang Ye , Cui Xiuming , Zhang Jinhao , Ji Guanghai , Wei Yunlin
Published: September 2023

Chryseobacterium indologenes is one of the primary causative agents of root rot of Panax notoginseng, which significantly affected plant growth and caused economic losses. With the increasing incidence of antibiotic-resistant bacterial phytopathogens, phage therapy has been garnered renewed attention in treating pathogenic bacteria. However, the therapeutic potential of phage therapy on root rot of P. notoginseng has not been evaluated. In this study, we isolated a novel lytic phage MA9V-1 infecting C. indologenes MA9 from sewage and monitored the formation of clear and round plaques with a diameter of approximately 0.5–1.5 mm. Phage MA9V-1 exhibited rapid absorption (>75% in 8 min), a latency period of 20 min, and a burst size of 10 particles per cell. Transmission electron microscopy indicated that the phage MA9V-1 is a new myovirus hosting C. indologenes MA9. Sequencing of phage genomes revealed that phage MA9V-1 contained a linear double-stranded DNA genome of 213,507 bp with 263 predicted open reading frames, including phage structure, host lysing, and DNA polymerase/helicase but no genes of tRNA, virulence, and antibiotic resistance. Our proteomic tree and genomic analysis revealed that phage MA9V-1 shares identity with Sphingomonas phage PAU and Tenacibaculum phage PTm1; however, they also showed apparent differences. Further systemic evaluation using phage therapy experiments on P. notoginseng suggested that phage MA9V-1 can be a potential candidate for effectively controlling C. indologenes MA9 infection. Thus, we have presented a novel approach to solving root rot in P. notoginseng.
The distribution variation of pathogens and virulence factors in different geographical populations of giant pandas
Authors: Zhao Mengyu , Li Yuxia , Wei Wei , Zhang Zejun , Zhou Hong
Published: September 2023

Intestinal diseases caused by opportunistic pathogens seriously threaten the health and survival of giant pandas. However, our understanding of gut pathogens in different populations of giant pandas, especially in the wild populations, is still limited. Here, we conducted a study based on 52 giant panda metagenomes to investigate the composition and distribution of gut pathogens and virulence factors (VFs) in five geographic populations (captive: GPCD and GPYA; wild: GPQIN, GPQIO, and GPXXL). The results of the beta-diversity analyzes revealed a close relationship and high similarity in pathogen and VF compositions within the two captive groups. Among all groups, Proteobacteria, Firmicutes, and Bacteroidetes emerged as the top three abundant phyla. By using the linear discriminant analysis effect size method, we identified pathogenic bacteria unique to different populations, such as Klebsiella in GPCD, Salmonella in GPYA, Hafnia in GPQIO, Pedobacter in GPXXL, and Lactococcus in GPQIN. In addition, we identified 12 VFs that play a role in the intestinal diseases of giant pandas, including flagella, CsrA, enterobactin, type IV pili, alginate, AcrAB, capsule, T6SS, urease, type 1 fimbriae, polar flagella, allantoin utilization, and ClpP. These VFs influence pathogen motility, adhesion, iron uptake, acid resistance, and protein regulation, thereby contributing to pathogen infection and pathogenicity. Notably, we also found a difference in virulence of Pseudomonas aeruginosa between GPQIN and non-GPQIN wild populations, in which the relative abundance of VFs (0.42%) of P. aeruginosa was the lowest in GPQIN and the highest in non-GPQIN wild populations (GPXXL: 23.55% and GPQIO: 10.47%). In addition to enhancing our understanding of gut pathogens and VFs in different geographic populations of giant pandas, the results of this study provide a specific theoretical basis and data support for the development of effective conservation measures for giant pandas.
The p53 tumor suppressor regulates AKR1B1 expression, a metastasis-promoting gene in breast cancer
Authors: Di Benedetto Carolina , Borini Etichetti Carla , Cocordano Nabila , Cantoia Alejo , Arel Zalazar Evelyn , Bicciato Silvio , Menacho-Márquez Mauricio , Rosano Germán Leandro , Girardini Javier
Published: September 2023

Alteration of metabolism in cancer cells is a central aspect of the mechanisms that sustain aggressive traits. Aldo–keto reductase 1 B1 (AKR1B1) catalyzes the reduction of several aldehydes to alcohols consuming NADPH. Nevertheless, the ability of AKR1B1 to reduce different substrates renders difficult to comprehensively ascertain its biological role. Recent evidence has implicated AKR1B1 in cancer; however, the mechanisms underlying its pro-oncogenic function remain largely unknown. In this work, we report that AKR1B1 expression is controlled by the p53 tumor suppressor. We found that breast cancer patients bearing wild-type TP53 have reduced AKR1B1 expression. In cancer cell lines, p53 reduced AKR1B1 mRNA and protein levels and repressed promoter activity in luciferase assays. Furthermore, chromatin immunoprecipitation assays indicated that p53 is recruited to the AKR1B1 promoter. We also observed that AKR1B1 overexpression promoted metastasis in the 4T1 orthotopic model of triple-negative breast cancer. Proteomic analysis of 4T1 cells overexpressing AKR1B1 showed that AKR1B1 exerts a marked effect on proteins related to metabolism, with a particular impact on mitochondrial function. This work provides novel insights on the link between the p53 pathway and metabolism in cancer cells and contributes to characterizing the alterations associated to the pathologic role of AKR1B1.
Interneuron odyssey: molecular mechanisms of tangential migration
Authors: Toudji Ikram , Toumi Asmaa , Chamberland Émile , Rossignol Elsa
Published: September 2023

Cortical GABAergic interneurons are critical components of neural networks. They provide local and long-range inhibition and help coordinate network activities involved in various brain functions, including signal processing, learning, memory and adaptative responses. Disruption of cortical GABAergic interneuron migration thus induces profound deficits in neural network organization and function, and results in a variety of neurodevelopmental and neuropsychiatric disorders including epilepsy, intellectual disability, autism spectrum disorders and schizophrenia. It is thus of paramount importance to elucidate the specific mechanisms that govern the migration of interneurons to clarify some of the underlying disease mechanisms. GABAergic interneurons destined to populate the cortex arise from multipotent ventral progenitor cells located in the ganglionic eminences and pre-optic area. Post-mitotic interneurons exit their place of origin in the ventral forebrain and migrate dorsally using defined migratory streams to reach the cortical plate, which they enter through radial migration before dispersing to settle in their final laminar allocation. While migrating, cortical interneurons constantly change their morphology through the dynamic remodeling of actomyosin and microtubule cytoskeleton as they detect and integrate extracellular guidance cues generated by neuronal and non-neuronal sources distributed along their migratory routes. These processes ensure proper distribution of GABAergic interneurons across cortical areas and lamina, supporting the development of adequate network connectivity and brain function. This short review summarizes current knowledge on the cellular and molecular mechanisms controlling cortical GABAergic interneuron migration, with a focus on tangential migration, and addresses potential avenues for cell-based interneuron progenitor transplants in the treatment of neurodevelopmental disorders and epilepsy.
Examining cortical tracking of the speech envelope in post-stroke aphasia
Authors: Quique Yina M. , Gnanateja G. Nike , Dickey Michael Walsh , Evans William S. , Chandrasekaran Bharath
Published: September 2023

Introduction

People with aphasia have been shown to benefit from rhythmic elements for language production during aphasia rehabilitation. However, it is unknown whether rhythmic processing is associated with such benefits. Cortical tracking of the speech envelope (CTenv) may provide a measure of encoding of speech rhythmic properties and serve as a predictor of candidacy for rhythm-based aphasia interventions.

Methods

Electroencephalography was used to capture electrophysiological responses while Spanish speakers with aphasia (n = 9) listened to a continuous speech narrative (audiobook). The Temporal Response Function was used to estimate CTenv in the delta (associated with word- and phrase-level properties), theta (syllable-level properties), and alpha bands (attention-related properties). CTenv estimates were used to predict aphasia severity, performance in rhythmic perception and production tasks, and treatment response in a sentence-level rhythm-based intervention.

Results

CTenv in delta and theta, but not alpha, predicted aphasia severity. Neither CTenv in delta, alpha, or theta bands predicted performance in rhythmic perception or production tasks. Some evidence supported that CTenv in theta could predict sentence-level learning in aphasia, but alpha and delta did not.

Conclusion

CTenv of the syllable-level properties was relatively preserved in individuals with less language impairment. In contrast, higher encoding of word- and phrase-level properties was relatively impaired and was predictive of more severe language impairments. CTenv and treatment response to sentence-level rhythm-based interventions need to be further investigated.
Efficient human activity recognition with spatio-temporal spiking neural networks
Authors: Li Yuhang , Yin Ruokai , Kim Youngeun , Panda Priyadarshini
Published: September 2023

In this study, we explore Human Activity Recognition (HAR), a task that aims to predict individuals' daily activities utilizing time series data obtained from wearable sensors for health-related applications. Although recent research has predominantly employed end-to-end Artificial Neural Networks (ANNs) for feature extraction and classification in HAR, these approaches impose a substantial computational load on wearable devices and exhibit limitations in temporal feature extraction due to their activation functions. To address these challenges, we propose the application of Spiking Neural Networks (SNNs), an architecture inspired by the characteristics of biological neurons, to HAR tasks. SNNs accumulate input activation as presynaptic potential charges and generate a binary spike upon surpassing a predetermined threshold. This unique property facilitates spatio-temporal feature extraction and confers the advantage of low-power computation attributable to binary spikes. We conduct rigorous experiments on three distinct HAR datasets using SNNs, demonstrating that our approach attains competitive or superior performance relative to ANNs, while concurrently reducing energy consumption by up to 94%.
Multicentric 68Ga-PSMA PET radiomics for treatment response assessment of 177Lu-PSMA-617 radioligand therapy in patients with metastatic castration-resistant prostate cancer
Authors: Gutsche Robin , Gülmüs Gizem , Mottaghy Felix M. , Gärtner Florian , Essler Markus , von Mallek Dirk , Ahmadzadehfar Hojjat , Lohmann Philipp , Heinzel Alexander
Published: September 2023

Objective

The treatment with 177Lutetium PSMA (177Lu-PSMA) in patients with metastatic castration-resistant prostate cancer (mCRPC) has recently been approved by the FDA and EMA. Since treatment success is highly variable between patients, the prediction of treatment response and identification of short- and long-term survivors after treatment could help tailor mCRPC diagnosis and treatment accordingly. The aim of this study is to investigate the value of radiomic parameters extracted from pretreatment 68Ga-PSMA PET images for the prediction of treatment response.

Methods

A total of 45 mCRPC patients treated with 177Lu-PSMA-617 from two university hospital centers were retrospectively reviewed for this study. Radiomic features were extracted from the volumetric segmentations of metastases in the bone. A random forest model was trained and validated to predict treatment response based on age and conventionally used PET parameters, radiomic features and combinations thereof. Further, overall survival was predicted by using the identified radiomic signature and compared to a Cox regression model based on age and PET parameters.

Results

The machine learning model based on a combined radiomic signature of three features and patient age achieved an AUC of 0.82 in 5-fold cross-validation and outperformed models based on age and PET parameters or radiomic features (AUC, 0.75 and 0.76, respectively). A Cox regression model based on this radiomic signature showed the best performance to predict overall survival (C-index, 0.67).

Conclusion

Our results demonstrate that a machine learning model to predict response to 177Lu-PSMA treatment based on a combination of radiomics and patient age outperforms a model based on age and PET parameters. Moreover, the identified radiomic signature based on pretreatment 68Ga-PSMA PET images might be able to identify patients with an improved outcome and serve as a supportive tool in clinical decision making.
Risk of second primary cancers in patients with rectal neuroendocrine neoplasms: a surveillance, epidemiology, and end results analysis
Authors: Wan Ming , Wu Jiaqi , Jiang Zhaopeng , Gong Wushuang , Zhou Xianli
Published: September 2023

Background

While an elevated risk of second primary cancers (SPCs) has been observed in many other cancers, risk of SPCs has not been quantified in patients with rectal neuroendocrine neoplasms (NENs).

Methods

Survivors of primary rectal NENs diagnosed between 2000 and 2018 were identified from the Surveillance, Epidemiology, and End Results (SEER)-18 registries. Relative risk of SPCs was estimated as the standardized incidence ratio (SIR), which was calculated using SEER*Stat software.

Results

Between 2000 and 2018, a total of 15836 patients diagnosed with rectal NENs, of whom 1436 (9.1%) received diagnosis of SPCs (SIR: 1.19, 95%CI: 1.13-1.26). The majority of patients were aged 50-69 and had their first cancer diagnosed at the localized stage. Male survivors had a higher propensity for developing SPCs overall, while female survivors exhibited higher risks of specific SPCs. Age at diagnosis of rectal NENs influenced the risk of SPCs, with younger patients having greater risks. A statistically significant increase in the incidence of SPCs was observed among patients aged 30-64 years. Black patients had higher relative risks of certain SPCs, while White patients had a lower risk of subsequent melanoma. Trend analysis revealed that the highest excess burden of SPCs was observed in the years 2000 to 2002. Risk of SPCs remained elevated within the first four years post-diagnosis for survivors of rectal NENs, but diminished thereafter.

Conclusion

The study revealed that individuals who survived rectal NENs were at an elevated risk of developing SPCs compared to the general population. Our results hold important implications for the formulation of lifelong surveillance recommendations for cancer survivors.
The effect of Shengmai injection in patients with coronary heart disease in real world and its personalized medicine research using machine learning techniques
Authors: Ma Jing , Yu Ze , Chen Ting , Li Ping , Liu Yan , Chen Jihui , Lyu Chunming , Hao Xin , Zhang Jinyuan , Wang Shuang , Gao Fei , Zhang Jian , Bu Shuhong
Published: September 2023

Objective: Shengmai injection is a common treatment for coronary heart disease. The accurate dose regimen is important to maximize effectiveness and minimize adverse reactions. We aim to explore the effect of Shengmai injection in patients with coronary heart disease based on real-world data and establish a personalized medicine model using machine learning and deep learning techniques.

Methods: 211 patients were enrolled. The length of hospital stay was used to explore the effect of Shengmai injection in a case-control study. We applied propensity score matching to reduce bias and Wilcoxon rank sum test to compare results between the experimental group and the control group. Important variables influencing the dose regimen of Shengmai injection were screened by XGBoost. A personalized medicine model of Shengmai injection was established by XGBoost selected from nine algorithm models. SHapley Additive exPlanations and confusion matrix were used to interpret the results clinically.

Results: Patients using Shengmai injection had shorter length of hospital stay than those not using Shengmai injection (median 10.00 days vs. 11.00 days, p = 0.006). The personalized medicine model established via XGBoost shows accuracy = 0.81 and AUC = 0.87 in test cohort and accuracy = 0.84 and AUC = 0.84 in external verification. The important variables influencing the dose regimen of Shengmai injection include lipid-lowering drugs, platelet-lowering drugs, levels of GGT, hemoglobin, prealbumin, and cholesterol at admission. Finally, the personalized model shows precision = 75%, recall rate = 83% and F1-score = 79% for predicting 40 mg of Shengmai injection; and precision = 86%, recall rate = 79% and F1-score = 83% for predicting 60 mg of Shengmai injection.

Conclusion: This study provides evidence supporting the clinical effectiveness of Shengmai injection, and established its personalized medicine model, which may help clinicians make better decisions.
Safety assessment of fluorescently labeled anti-EGFR Nanobodies in healthy dogs
Authors: Valle Nayra Cristina Herreira do , Janssen Simone , Stroet Marcus C. M. , Pollenus Sofie , Van den Block Sonja , Devoogdt Nick , Debacker Jens M. , Hernot Sophie , De Rooster Hilde
Published: September 2023

Introduction: Surgical resection is one of the main treatment options for several types of cancer, the desired outcome being complete removal of the primary tumor and its local metastases. Any malignant tissue that remains after surgery may lead to relapsing disease, negatively impacting the patient’s quality of life and overall survival. Fluorescence imaging in surgical oncology aims to facilitate full resection of solid tumors through the visualization of malignant tissue during surgery, following the administration of a fluorescent contrast agent. An important class of targeting molecules are Nanobodies® (Nbs), small antigen-binding fragments derived from camelid heavy chain only antibodies. When coupled with a fluorophore, Nbs can bind to a specific receptor and demarcate tumor margins through a fluorescence camera, improving the accuracy of surgical intervention. A widely investigated target for fluorescence-guided surgery is the epidermal growth factor receptor (EGFR), which is overexpressed in several types of tumors. Promising results with the fluorescently labeled anti-EGFR Nb 7D12-s775z in murine models motivated a project employing the compound in a pioneering study in dogs with spontaneous cancer.

Methods: To determine the safety profile of the study drug, three healthy purpose-bred dogs received an intravenous injection of the tracer at 5.83, 11.66, and 19.47 mg/m2, separated by a 14-day wash-out period. Physical examination and fluorescence imaging were performed at established time points, and the animals were closely monitored between doses. Blood and urine values were analyzed pre- and 24 h post administration.

Results: No adverse effects were observed, and blood and urine values stayed within the reference range. Images of the oral mucosa, acquired with a fluorescence imaging device (Fluobeam®), suggest rapid clearance, which was in accordance with previous in vivo studies.

Discussion: These are the first results to indicate that 7D12-s775z is well tolerated in dogs and paves the way to conduct clinical trials in canine patients with EGFR-overexpressing spontaneous tumors.
From a Co-Mo precursor to 1H and 1T Co-promoted MoS2: exploring the effects of gas pressure
Authors: Prabhu M. K. , Groot I. M. N.
Published: September 2023

The work presented in this paper makes use of the high-pressure in situ imaging capabilities of the ReactorSTM to demonstrate that single layer 1T Co-promoted MoS2 can be directly synthesized without the use of any intercalating agents by applying highly reducing conditions during the growth. In this work, we have sulfided a CoMo nanoparticle precursor supported on Au(111) using a H2:CH3SH gas mixture at 1 bar and imaged the crystallization process in situ using the ReactorSTM. We have observed that at low temperatures (∼500 K), an intermediate disordered CoMoSx phase is formed which crystallizes into metallic single-layer 1T Co-promoted MoS2 slabs at temperatures close to 600 K. We also show that semiconducting 1H Co-promoted MoS2 slabs synthesized under sulfur-rich conditions using a vacuum physical vapor deposition process, do not transform into their metallic 1T counterparts when exposed to the same reducing gas pressures and temperatures, thus, demonstrating the importance of the highly reducing conditions during the crystallization process for inducing the formation of the metastable 1T phase. XPS spectra of the 1T Co-promoted MoS2 slabs indicate a sulfur deficiency of up to 11% in the top layer S, suggesting the likely role of sulfur vacancies in the formation of the 1T phase.
Soil acidification and salinity: the importance of biochar application to agricultural soils
Authors: Huang Kai , Li Mingquan , Li Rongpeng , Rasul Fahd , Shahzad Sobia , Wu Changhong , Shao Jinhua , Huang Guoqin , Li Ronghui , Almari Saad , Hashem Mohamed , Aamer Muhammad
Published: September 2023

Soil acidity is a serious problem in agricultural lands as it directly affects the soil, crop production, and human health. Soil acidification in agricultural lands occurs due to the release of protons (H+) from the transforming reactions of various carbon, nitrogen, and sulfur-containing compounds. The use of biochar (BC) has emerged as an excellent tool to manage soil acidity owing to its alkaline nature and its appreciable ability to improve the soil’s physical, chemical, and biological properties. The application of BC to acidic soils improves soil pH, soil organic matter (SOM), cation exchange capacity (CEC), nutrient uptake, microbial activity and diversity, and enzyme activities which mitigate the adverse impacts of acidity on plants. Further, BC application also reduce the concentration of H+and Al3+ions and other toxic metals which mitigate the soil acidity and supports plant growth. Similarly, soil salinity (SS) is also a serious concern across the globe and it has a direct impact on global production and food security. Due to its appreciable liming potential BC is also an important amendment to mitigate the adverse impacts of SS. The addition of BC to saline soils improves nutrient homeostasis, nutrient uptake, SOM, CEC, soil microbial activity, enzymatic activity, and water uptake and reduces the accumulation of toxic ions sodium (Na+and chloride (Cl-). All these BC-mediated changes support plant growth by improving antioxidant activity, photosynthesis efficiency, stomata working, and decrease oxidative damage in plants. Thus, in the present review, we discussed the various mechanisms through which BC improves the soil properties and microbial and enzymatic activities to counter acidity and salinity problems. The present review will increase the existing knowledge about the role of BC to mitigate soil acidity and salinity problems. This will also provide new suggestions to readers on how this knowledge can be used to ameliorate acidic and saline soils.
Examining the differential effects of information about the death penalty on retributivists and non-retributivists in Japan: a refutation of Marshall's third hypothesis
Authors: Watamura Eiichiro , Ioku Tomohiro , Mukai Tomoya
Published: September 2023

This study aimed to test Marshall's third hypothesis—that information about the death penalty hardly affects the attitude of death penalty supporters on retribution grounds—utilizing a non-American sample. Four pre-registered experiments were conducted, involving Japanese participants randomly selected from sample pools of retributivists and non-retributivists, based on their reasons for supporting the death penalty. One group received information exposure, while the other was under control conditions. Participants read about deterrence (Study 1) or false convictions (Study 2–4). Except for the results of Study 4, retributivists and non-retributivists were equally affected or unaffected by information. Marshall's third hypothesis is therefore not supported. Retributivists strongly favored the death penalty; higher empathy toward criminals was associated with less pro-death penalty attitudes. Additionally, there were differences in the influence of information. These results suggest the need for a new approach to researching the relationship between public attitudes and information on the death penalty.
Development and adaptation of a strength-based job interview training tool for transition age youth on the autism spectrum using community engaged methods
Authors: Genova Helen M. , Haas Mikayla , Chen Yu-Lun , Elsayed Heba E. , McGrath Robert E. , Smith Matthew J.
Published: September 2023

Introduction

Kessler Foundation Strength Identification and Expression (KF-STRIDE) is a strength-based job interview training tool developed for young adults on the autism spectrum. The intervention is based on a positive psychology framework to increase knowledge of character strengths, and how to relate them to a future employer. The current study sought to evaluate the acceptability, usability and feasibility of KF-STRIDE, as well as to guide adaptations to improve the tool’s ability to meet the needs of those on the spectrum.

Methods

Mixed methods (post-intervention surveys, and semi-structured interviews with key stakeholders) were used to inform the evaluation and consequent adaptations of KF-STRIDE.

Results

The major findings of the study were that KF-STRIDE was found to be largely acceptable and usable. Importantly, however, our qualitative analysis revealed modifications that could help to better suit the needs of young adults on the spectrum, which included the incorporation of additional skills (i.e. etiquette, practicing hygiene) and more opportunities to practice job interviewing. Thus, we altered the implementation of the intervention to be web-based to improve accessibility. We incorporated the presence of an animated character to deliver the content, to eliminate the need for a highly trained interventionist.

Discussion

KF-STRIDE was modified to increase access by incorporating feedback from the autism community. Future directions include assessing the efficacy of KF-STRIDE in young adults on the spectrum to identify whether employment outcomes are improved after using the tool.
Epidemiology of human papillomavirus-associated anogenital cancers in Granada: a three-decade population-based study
Authors: Dabán-López Pablo , Fernández-Martínez Nicolás Francisco , Petrova Dafina , Rodríguez-Barranco Miguel , Jiménez-Moleón Jose Juan , Gutierrez Javier , Sánchez María-José
Published: September 2023

Introduction

HPV infection is a common risk factor for all anogenital cancers. However, there are important differences in the epidemiology of anogenital cancers and these have not been compared considering diverse epidemiological indicators over a long period of time. To fill this gap, we investigated incidence, mortality, and survival trends of anogenital cancers over a period of three decades.

Methods

We conducted an observational registry-based study using data from the population-based cancer registry of Granada in southern Spain. We collected data on all incident cases of anogenital cancer (cervical, anal, penile, vulvar, and vaginal cancer) diagnosed between 1985 and 2017. We calculated crude and age-standardized incidence and mortality rates, and 1, 3, and 5-year overall and net survival. We further conducted time-trend analysis calculating annual percent changes (APC) for each cancer site.

Results

The incidence of anogenital cancers decreased slightly during the past 30 years, with the exception of vulvar cancer, where a slight increase was observed. Mortality decreased significantly for cervical cancer over the study period but increased non-significantly for the remaining cancer sites. Survival rates were similar to those reported in comparable countries and increased for cervical and vulvar cancer.

Discussion

Cervical cancer was the greatest contributor to the burden of anogenital cancers and showed a marked improvement in all indicators in comparison to the remaining cancer sites.
Traditional food products on the local market - consumption conditional on the characteristics of management and restaurant facilities in tourism of Vojvodina (Serbia)
Authors: Kalenjuk Pivarski Bojana , Tekić Dragana , Šmugović Stefan , Novaković Aleksandra , Ivanović Velibor , Petrović Marica , Banjac Maja , Đerčan Bojan , Tešanović Dragan , Ćirić Miloš , Čabarkapa Ivana , Ćirić Ivana , Šarac Veljko , Maravić Nikola
Published: September 2023

The research dealt with traditional food products (TFP) observed on the local hospitality-touristic market, from the point of view of 300 restaurant workers employed in managerial positions, as important subjects in preservation of tradition in hospitality and touristic industry of Vojvodina (Republic of Serbia). The research examined the management’s views on TFP, as well as whether there are differences based on the features of the respondents as well as the restaurant facility. In order to obtain data that would provide additional guidelines for acting on the hospitality and tourism market, the procurement and consumption of TFP was also examined. In order to determine set differences the non-parametric Kruskal-Wallis test was applied. In order to establish statistically significant differences between the observed groups, the Dunn’s post hoc test was applied. In the second part of the research, a binary logistic regression model was applied in order to determine which of the observed variables has a significant impact on the respondents’ decision to acquire TFP from their field. The research showed that the level of education and the field of management education significantly influence the attitudes about the offer of TFP in the hospitality and tourism market, as well as the type of restaurant offer and the development of the field of business.