bluuebunny/crossref_metadata_2025 huggingface dataset:
Criminal Enforcement against Illegal Cosmetic Business Actors
Authors: Yusuf Elfira S
Published: August 2023

This study aimed to find out the Criminal Enforcement Against Illegal Cosmetic Business Actors in Gorontalo City. This type of research was empirical legal research, namely research by taking and using existing data in the field (primary) as the main data source, such as the results of interviews and observations. The Food and Drug Supervisory Agency (BPOM) should be even more assertive in providing a deterrent effect to mischievous business actors and must always carry out routine operations (surprise inspection) on business actors, not only through reports from the public and then officers going down to the field to carry out operations but must indeed be scheduled in a week how many times to do operations. If BPOM only waits for reports from the public, there will be no deterrent effect on mischievous business actors who have never been caught in raids/operations by the Food and Drug Supervisory Agency (BPOM).
Performance Effectiveness Of Mediators In Settlement Of Industrial Relations Disputes In Manpower And Transmigration Services
Authors: Daud Sarah Magfirah
Published: August 2023

The purpose of this study is to describe and identify the performance of mediators in resolving industrial relations disputes at the Manpower and Transmigration Office of Gorontalo Regency and to identify and analyze the inhibiting factors experienced by mediators in resolving industrial relations disputes if they fail. The research method used is sociological juridical, namely examining applicable legal provisions and what happens in society or research conducted on real conditions that occur in society. The results of this study are that the success of a mediation process depends on the performance of the mediator himself in the settlement of industrial relations disputes. According to the mediator at the Manpower and Transmigration Office of Gorontalo Regency, cases that are often handled and resolved at the mediation stage are cases of disputes over the termination of employment (PHK). In the mediation process, failure is something that is also found in reaching a collective agreement, because several internal and external factors make the mediation process unsuccessful, so it is recommended to go to court. The conclusion from the research is that the performance of the mediator in the settlement of industrial relations disputes at the Department of Manpower and Transmigration of Gorontalo Regency has been running according to the applicable regulations and has not been effective.
Perlindungan Hukum Terhadap Investor Dalam Transaksi Investasi Bodong (Dalam Perjanjian Investor Dengan Fx Family Di Kec. Batudaa)
Authors: Rahmawaty B. Wantu Nur
Published: August 2023

Tujuan dari penelitian ini adalah untuk mengetahui bagaimana perlindungan hukum terhadap investor dalam transaksi investasi bodong dalam perjanjian investor dengan admin fx family di kec. Batudaa Kab. Gorontalo dan mengetahui bagaimana mekanisme dalam perjanjian trading forex. Metode penelitian yang digunakan adalah metode penelitian hukum normatif empiris. Dengan menggunakan pendekatan undang-undang dan pendekatan kasus serta Data yang diperoleh dalam penelitian ini dianalisis secara kualitatif, yaitu dengan mengumpulkan data, mengkualifikasikan kemudian menghubungkan teori yang berhubungan dengan masalah dan menarik kesimpulan untuk menentukan hasil. Berdasarkan hasil penelitian mekanisme perjanjian Investasi Fx Family berskema ponzy (Multi Level Marketing) dan tidak mendapat izin dari OJK maupun BAPPEBTI (Bodong) dan bentuk perlindungan hukum yang diberikan kepada investor untuk mengantisipasi investasi ilegal adalah melalui instrumen hukum yang dikeluarkan oleh OJK misalnya Bidang Edukasi dan Perlindungan Konsumen yang bertugas untuk memberikan pengetahuan dan perlindungan kepada masyarakat serta adanya Layanan Sistem Pelayanan Konsumen Terintegrasi Sektor Jasa Keuangan (Financial Customer Care System) yang menerima pengaduan dari masyarakat melaui e-mail, faxmili maupun telepon. Penyelesaian kasus investasi ilegal Fx Family sekarang dilakukan oleh Disreskrinus Polda Gorontalo yang telah melimpahkan berkas kasus investasi bodong Fx Family ke Kejaksaan Tinggi Gorontalo.
Criminology Study of Sexual Violence in Children
Authors: Taha Oponu Khairunnisa , Cherawaty Thalib Mutia , Mantali Avelia
Published: August 2023

The purpose of this study is to determine the factors that cause sexual violence that occurs in children and to determine the efforts to overcome cases of sexual violence against children in Gorontalo Regency. The research method used is empirical legal research using a case approach and a conceptual approach. The results of the study found that there were five factors that caused cases of sexual violence against children in Gorontalo Regency, including: the lack of family responsibility, the lack of social control from the community, the technology factor, the alcohol factor, the lack of understanding and the inculcation of norms in the order of life. . The most dominant factors according to the percentage of cases of sexual violence against children in Gorontalo Regency are the lack of parental responsibility and technology (social media) factors. Efforts to prevent and overcome cases of sexual violence against children in Gorontalo Regency are carried out with preventive efforts, namely a form of prevention before the occurrence of cases of sexual violence against children. Preventive efforts are divided into five efforts, including: applying self-awareness, active communication with family, using technology as best as possible, choosing a good environment, socialization from related parties. The other efforts are repressive efforts, one of the forms of efforts to overcome when there have been cases of sexual violence against children. Repressive efforts are carried out by providing criminal sanctions for crimes that have been committed by the perpetrators. As well as repressive efforts to victims by providing psychological assistance and legal assistance.
Cockfighting Gambling Criminal Acts Commitment
Authors: Lasena Maya , U. Puluhulawa Fenty , M. Wantu Fence , Ahmad Ahmad
Published: August 2023

This study aims to determinea form of tackling the crime of cockfighting gambling.The method used in this research is empirical or sociological research methodology. This study uses a qualitative approach and sampling using purposive sampling and using descriptive analysis techniques. The results of this study indicate thata form of countermeasure carried out to tackle the crime of cockfighting gambling by means of preventive and repressive measures. In the preventive action stage, basically, stakeholders try to take precautions before the crime occurs. However, the most important role in preventing the occurrence of a crime is the supervision of the local community who then cooperates with law enforcement so that the crime of cockfighting gambling is easily detected.The countermeasures carried out by the authorities should be carried out with good coordination and cooperation, so as to make countermeasures that are right on target.
Fulfillment Of Assimilation Rights And Integration Rights To Corporate Students
Authors: Bau Susan , Abdussamad Zamroni , Mustika Waode
Published: August 2023

This study aims to determine the fulfillment of assimilation rights and integration rights to correctional students. The type of research method used in this study is empirical research with sampling using purposive sampling. This study also uses a qualitative approach and descriptive analysis techniques. The results of this study indicate thatIn the implementation of the fulfillment of the right of assimilation and the right of integration to correctional students, there are several obstacles, as for these obstacles, namely there is no guarantor of the correctional students, has register f records, Community Environment, excess capacity, minimal quality of officers and less bureaucratic processes. effective. The government carries out alternative non-prison punishments that can be carried out such as supervision and community service which is seen as more efficient than prison law because in achieving the goal of fostering or rehabilitating correctional students before finally being able to be free and return to life in the community and for the community.The community is expected to participate actively in the implementation of community programs, so that correctional students feel they can be accepted again in the community.
Debatable of Indonesian Citizens' Rights and Obligations Regarding the Covid-19 Vaccination Policy
Authors: Saman Faizal
Published: August 2023

The purpose of this study is to examine the rights and obligations of Indonesian citizens regarding vaccination.  This research uses a type of normative legal research with a statute approach.  Collect data by grouping and reviewing regulations, library materials, books, and other sources related to problems in this study.  The results showed that polemics that led to pros and cons related to vaccination were caused by several factors such as vaccines being new, the safety of vaccines that cannot be passed, and the sanctions imposed on those who refuse vaccines.  The pro-life community considers vaccination to be an obligation and the contras think that the imposition of vaccination is in line with the right to health.  There are several variables for the reason for the vaccination policy to be implemented, namely the state in a state of emergency, namely in a  pandemic crisis, and subsequently related to the human obligation to respect the human rights of others (the right to the health of others).  Thus, the problem of legal certainty from the implementation of vaccination is an obligation of Indonesian citizens.
Black Stone Mining (Effort to Control Environmental Damage)
Authors: Demanto Zulkifli H. Demanto
Published: August 2023

This study discusses black stone mining in Bone Bolango Regency, Gorontalo Province. The type of research used in this research is sociological or empirical legal research. This research uses a normative legal science approach (laws and regulations). The author finds that the legal consequences arising from the illegal mining of black stone in Bone Bolango Regency is a criminal act in the mining sector prohibited by regulations, and the authors find that the role of the District Government. Bone Bolango in preventing environmental damage due to black stone mining activities consists of 3 things, namely; 1). Reclamation; 2). Periodic checking and monitoring; 3). Providing education to the community. Based on the explanation in the discussion and conclusions that the authors have described above, the authors have recommendations for suggestions to the government as follows; 1). Local governments must actively participate in protecting the environment in cases of damage caused by illegal mining activities; 2). The government must formulate appropriate regulations to protect the rights and interests of all parties.
The Disparity in Judge's Decisions in Forced Defense Cases
Authors: Katili Nur Amalia , Ekawaty Ismail Dian , Yutye Imran Suwitno
Published: August 2023

This study aims to analyze the chronology of cases of forced defense decisions. Normative research methods using statutory research approaches and case approaches and analytical techniques used to process legal materials are legal hermeneutics. The results of this study indicate that the decisions 794/Pid.B/2014/PN.Llg and No.257/Pid.B/2015/PN.Sky has fulfilled the elements of the noodweer requirement, namely that defense is coercive, what is being defended is oneself ), there was a very imminent threat of an imminent attack at that time, and that attack was against the law. has also fulfilled the element of the principle of subsidiarity which is the benchmark for forced defense. The disparity of decision No. 794/Pid.B/2014/PN.Log and the decision No. 257/Pid.B/2015/PN.
Legal Implications of Differences in Goods Price Labels in Shop Cashier Showcases for Entrepreneurs
Authors: Laboro Suci , Elfikri Nurul Fazri
Published: August 2023

The purpose of this research is to know and analyze about iThe legal implication is that there is a difference in the price tag of goods on the window display and the shop cashier for entrepreneurs, which has implications for the provisions of the Consumer Protection Act and Permendag No. 35 of 2013 which can result in criminal sanctions and administrative sanctions as well as payment based on the lowest price tag. Based on the aspect of responsibility as a business actor, namely public accountability and private responsibility, it has implications for law enforcement (criminal sanctions and administrative sanctions). The problem of price differences in shop windows and during the payment process at the cashier also has implications for the course of the buying and selling process in social life, among other things, has implications for price uncertainty, injustice for consumers, and has implications for benefits that only benefit business actors...
Responsibility of the Default Debtor in the Implementation of the Credit Agreement and Settlement
Authors: Yasin Susinta , Almoravid Dungga Wen , T Mandjo Julius
Published: August 2023

This article discusses the provision of credit provided by banks, one of which is at the Telaga Unit BRI bank with a building guarantee. Where at the Telaga Unit BRI bank in providing credit previously preceded by an agreement. To enter into an agreement, the borrower (customer) must have collateral. Without collateral, a customer cannot obtain a loan because collateral is one of the requirements for the customer to enter into a loan agreement. There is a close relationship between the lender and the guarantee. To guarantee repayment of credit from the debtor.
Implementation of Criminal Liability for Companies That Do Not Fulfill Obligations to Workers in Employment BPJS Guarantee
Authors: Dawali Moh. Fahrul , W. Badu Lisnawati , Y. Imran Suwitno
Published: August 2023

This study aims to determine and analyze the criminal liability of companies that do not fulfill their obligations to workers in the employment BPJS guarantee. The research method used is sociological juridical research, using a descriptive qualitative research approach and interactive analysis techniques. The results of this study indicate that there are still many companies that neglect to provide fulfillment of workers' obligations in the Employment BPJS guarantee as stipulated in Law No. 24 of 2011 concerning the Social Security Organizing Agency (Law No. 24 of 2011), which protects workers' rights to obtain guarantees for compensation for work accidents. The establishment of the Social Security Organizing Agency (BPJS) is not a new thing in the field of employment, because social security for workers has previously been regulated in Law No. 14 of 1993 concerning Workers' Social Security or better known as Jamsostek. Law No. 40 of 2004 on the National Social Security System was enacted in 2004 with the aim of creating an integrated social security system for all Indonesians, and the social security system was institutionalized in a public legal entity.
Analysis of the Legal Power of SKPT in Land Sale and Purchase Transactions in Paleleh District, Buol Regency
Authors: Basiman Moh. Yoenardi M. , Junus Nirwan , Meiske Kamba Sri Nanang
Published: August 2023

The purpose of this research is to find out the legal force of SKPT in land sale and purchase transactions in Paleleh District, Buol Regency. The research method used is empirical research, namely the author will seek and find information which will later be used as the substance of this research by means of observation or conducting field studies in the place that is used as the object of this research. this research is carried out in a planned and systematic manner to obtain answers to problem solving related to the problems that occur, especially regarding the existence of buying and selling transactions using Land Tenure Certificate (SKPT) in Paleleh District. The results of this research on the legal force of land ownership certificates in sale and purchase transactions in Paleleh Subdistrict, where many people in Paleleh Subdistrict conduct land sale and purchase transactions using only a Land Ownership Certificate.  This is due to the habits of people in rural areas who do not want to be complicated in thinking about the legal aspects of land sale and purchase transactions, for them it is enough just to provide receipts as proof that payment has been made.
Analysis of Judge's Decision in Imposing Child Abuse Crime
Authors: Sauri Husnulhotima
Published: August 2023

The purpose of this research is to know and analyze abouthow to Analyze the Decision of the Gorontalo High Court Number: 2/Pid-Sus-Anak/2020/PT Gto against children as perpetrators of criminal acts of obscenity in the city of Gorontalo and to find out what factors influence the Judge's Decision in Cases of Crime of Child Abuse in Court High Gorontalo Number: 2/Pid-Sus-Children/2020/PT Gto. The type of approach used is Normative Research where the approach used is a statute approach and a case approach. The data obtained are from literature, laws, journals, and thesis. Data analysis or data management in this study was carried out in a deductive way. The results obtained in this study indicate that the judge in imposing a sentence on the perpetrator is still contrary to the principle of proportionality. Then there are several factors that influence the judge's decision, which include: 1.) Factors originating from the judge. 2) Factors originating from the defendant.
Return of State Losses In The Investigation Process That Can Eliminate Criminal Perpetrators of Corruption
Authors: Doda Andi Mirzan , M. Wantu Fence , Ekawaty Ismail Dian
Published: August 2023

The purpose of writing to be achieved in this paper is to know and analyze the return of state losses in the investigation process that can eliminate criminal perpetrators of corruption.  This research is a normative legal research using  the statutory approach "statute approach", the case approach "case approach", and the  conceptual approach "conceptual approach".  This study used analytical descriptive analysis techniques. The results of this study show that the return of state financial losses in the investigation stage of corruption can stop the process of handling criminal acts through police discretion. One of the elements of corruption is the element of state loss, meaning that when it has been returned, it means that the element has been lost, meaning there is no loss as a logical consequence of the Constitutional Court decision No. 25/PUU-XIV/2016. But with the condition that it must be before the investigation stage through police discretion and if it is based on  the Lex Posterior Derogate Lege Priori, meaning that the new law overrides the old  law, meaning that the new regulation ignores or overrides the old regulation in the same respect
Law Enforcement Efforts for Child Murder
Authors: Latief Wahyu
Published: August 2023

The purpose of this study was to find out what are the factors that cause children to commit murder crimes, as well as to find out what efforts are made by the Gorontalo City Police in overcoming the occurrence of a murder crime committed by children. This research was conducted using empirical legal research methods. The results of this study can be seen that the factors that cause children to commit murder are internal factors which include unstable emotional factors, misunderstanding factors, vengeful factors, psychological factors, and weak faith factors. While on external factors, namely family factors, economic factors, alcoholic beverage factors, and child neglect factors. While the countermeasures carried out by the Gorontalo City Police are pre-emptive efforts which are basic actions in the form of appeals, then preventive efforts are continued efforts preemptive in the form of direct and repressive preventive measures which are punitive efforts or law enforcement efforts
Socio Juridical Review Of The Criminal Action Of Embraction Of Fiduciary Objects (Case Study Of Kabila District, Bone Bolango District )
Authors: Abdjul Hadrian
Published: August 2023

This research aim to find out Socio - juridical review of the crime of embezzlement of Fiduciary Guarantee objects . This study raises two issues, namely how the socio-juridical review of the crime of embezzlement of Fiduciary Guarantee objects and what are the causative factors. This study uses an empirical legal research method with a sociological jurisprudence approach . The results of this study indicate that the socio-juridical review of the crime of embezzlement of Fiduciary Guarantee objects in Kabila District, Bone Bolango Regency is based on the socio-juridical scope, namely, first the law, Second , state institutions that function to form or make and enforce the law , and Third , the interactive relationship between the formal legal system and the legal order of society. Factors causing the crime of embezzlement of Fiduciary Guarantee objects, namely First, economic factors, where the provision of fees is one of the reasons KN is willing to lend his identity to be used in Fiduciary Guarantee agreements by AHI. The second is the social environmental factor, where the friendship relationship that exists between the perpetrators of embezzlement of Fiduciary Guarantee objects is one of the reasons KN is willing to lend his identity to be used by AHI in the Fiduciary Guarantee agreement.
Application of The Concept of Maslahah by Judges to Issuance of Marriage Dispensation Due to Pregnancy in Religious Courts
Authors: Kasim Nur Mohamad , Daud Indra Saputra
Published: August 2023

The purpose of the study was to determine the substance of the granting of marriage dispensation due to pregnancy and to describe the implementation factors of the provision of marriage dispensation due to pregnancy in the Religious Courts. The research method used is sociological juridical research using a qualitative approach and taking three research locations in the Gorontalo area, namely the Gorontalo Religious Court, the Suwawa Religious Court and the Limboto Religious Court. This study uses prescriptive legal analysis. The results of the study, namely, substantially the provision of marriage dispensation due to pregnancy, namely the judge's consideration regarding legal considerations governing and applying the concept of maslahah if marriage dispensation is accepted/rejected the implementation factor of granting marriage dispensation due to pregnancy is considering the fate of a child who, if born without status, is rejected if the request for a marriage dispensation is rejected.
Juxtaposing The Territorial Integrity and Self-Determination Principles in Nagorno-Karabakh Dispute
Authors: Manohara I Gusti Ayu Khanaya , Arsika I Made Budi , Darmasetiyawan I Made Sena
Published: August 2023

The separation of the former Soviet Union resulted in non civic divorces; ethnic cleansing, massacres, a territorial dispute which the Nagorno-Karabakh dispute stands as an example of. This conflict has been going on for years and is constantly evolving and regularly flares up resulting in casualties. The differing accusation of Armenia and Azerbaijan regarding the issue of the Nagorno-Karabakh conflict create different dimensions of the parties. Armenia sees the settlement as in the framework of self-determination whereas Azerbaijan pertains to the principle of territorial integrity in which both are the basic principles of international law thus leading to confusion regarding the legal dimensions of the conflict. With the most recent aggression, it is paramount now more than ever to seek the best possible settlement to put an end to the dispute. Until recently, Armenia accepts a deal with Russia to surrender the disputed territory by withdrawing its army. The main objective of this article is to give a general assessment of the disputed principles in this case
Cash Waqf Linked Sukuk Through Securities Crowdfunding in Indonesia
Authors: Aufa Imtiyaz Wizni , Pratama Anugrah Muhtarom , Pati Umi Khaerah
Published: August 2023

Islamic charitable innovations have evolved to give rise to the integration between Sukuk and cash waqf called Cash Waqf Linked Sukuk (CWLS). The integration of these two things is promising when combined, considering that Sukuk has long been named a commercial sharia instrument that aims to raise funds, while cash waqf as a sharia social instrument has played a philanthropic role. As for Indonesia, The cash waqf combined with Sukuk has become a new fiscal instrument because it has advantages in addition to contributing to development, furthermore, the returns obtained are reused into waqf money for socio-economic projects and funds invested be returned at maturity. As a legal research, this article uses qualitative analysis with statutory approaches and conceptual approaches. This article intends to introduce a new conceptual framework model innovation between CWLS and fintech types of Islamic securities crowdfunding. This innovation is for no reason considering that the use of fintech types of Islamic securities crowdfunding makes CWLS more popular and more flexible so that the funds raised are expected to be larger and can reach the broader community for the construction of more massive socio-economic projects. Furthermore, this article provides an overview of the proposed innovations in achieving the goals of maqasid al-shariah, regulations, and opportunities and challenges to be implemented in Indonesia.
Legal Protection of Rohingya Citizens Related to The Conflict in Myanmar
Authors: Putri Elfirda Ade , Wahyuni Windy Sri , Syaputra Muhammad Yusrizal Adi , Paramesvari Agata Jacqueline , Pratama Gede Aditya
Published: August 2023

There are many crimes against humanity cases that occurred in the world. One of them is happened in Myanmar, which is Rohingya ethnic conflict. Rohingya ethnic conflict is an issue based on discrimination to Rohingya ethnic because there are some differences at ethnic and religion. Ethnic Rohingya is not recognized by Myanmar and not granted a status of citizenship Based on Myanmar Citizenship Law (Burma Citizenship Law 1982). This research was conducted to find out how the legal forms to Rohingya citizens based on international law. This methodology is a normative legal research that uses statutes, case, fact approaches. The research found that the role of UNHCR in handling Rohingya cases has been mandated by the United Nations and in accordance with UNHCR Statute. UNHCR plays an important role in addressing the issue of Rohingya case, at leas as initiator, facilitator, conciliator, and determination. Such roles were taken to resolve the conflict that face by Rohingya ethnic, like provide protection, safety, and facilitate every Rohingyas refugees needs. In addition to the role of UNHCR, some countries such as Indonesia, Thailand and Canada also provided some arrangement in addressing the issue of Rohingya.
Perception of Indonesia and Afghanistan in Preventing Psychic Violence Against the Household Women
Authors: Sopacua Margie Gladies
Published: August 2023

The urgency and purpose of this study examine efforts to prevent psychological violence against women in the household as a form of upholding human rights. The method used is normative juridical, namely by studying theoretical literature, legal concepts, and legal norms, and connected with the efforts to prevent psychological violence against women in the household as a form of upholding human rights. The research results show that prevention psychological violence against women in the household as a form of upholding human rights, namely husbands and wives who are within the scope of the household should further increase their respective faiths by getting closer to God Almighty; Husbands and wives are open to each other, appreciate, respect and love within the scope of the household with full affection so that husbands and wives feel harmonious and comfortable both spiritually, physically, psychologically and so on; We must reject beliefs, beliefs or history about women that women are weak and as a wife if something happens in the household it becomes the wife's fault; Awareness to all parties included in the household sphere that domestic violence that occurs to women as wives is no longer a family disgrace, but acts of domestic violence are criminal acts and need to be handled properly and domestic violence must be abolished in every household sphere ; In order to escape from domestic violence, victims need to get legal protection and safe shelters prepared by the government.
MARKET OVERVIEW OF EARLY-MATURING CORN HYBRIDS FOR SILAGE PRODUCTION IN THE CENTRAL REGION OF RUSSIAN FEDERATION
Authors: Klimenko Vladimir , Belomozhnov Timofey
Published: August 2023

Corn silage is an important component of the rations for high productive dairy cattle due high nutritive value – about 30% of starch in the plants dry matter at harvesting in wax ripeness of grain. Specialized hybrids of corn are an excellent raw material for production of the fermented fodders with high energy concentration. The article presents market review of mid- and early-maturing corn hybrids, cultivated on farms in the Central region of the Russian Federation for silage over the past three years. The total 52 new early-maturing corn hybrids were included in the State Plant Register, but on the current moment the most part (about 60%) is the achievements of foreign breeding.
Recent progress on underwater soft robots: adhesion, grabbing, actuating, and sensing
Authors: Zhang Yeming , Kong Demin , Shi Yan , Cai Maolin , Yu Qihui , Li Shuping , Wang Kai , Liu Chuangchuang
Published: August 2023

The research on biomimetic robots, especially soft robots with flexible materials as the main structure, is constantly being explored. It integrates multi-disciplinary content, such as bionics, material science, mechatronics engineering, and control theory, and belongs to the cross-disciplinary field related to mechanical bionics and biological manufacturing. With the continuous development of various related disciplines, this area has become a hot research field. Particularly with the development of practical technologies such as 3D printing technology, shape memory alloy, piezoelectric materials, and hydrogels at the present stage, the functions and forms of soft robots are constantly being further developed, and a variety of new soft robots keep emerging. Soft robots, combined with their own materials or structural characteristics of large deformation, have almost unlimited degrees of freedom (DoF) compared with rigid robots, which also provide a more reliable structural basis for soft robots to adapt to the natural environment. Therefore, soft robots will have extremely strong adaptability in some special conditions. As a type of robot made of flexible materials, the changeable pose structure of soft robots is especially suitable for the large application environment of the ocean. Soft robots working underwater can better mimic the movement characteristics of marine life in the hope of achieving more complex underwater tasks. The main focus of this paper is to classify different types of underwater organisms according to their common motion modes, focusing on the achievements of some bionic mechanisms in different functional fields that have imitated various motion modes underwater in recent years (e.g., the underwater sucking glove, the underwater Gripper, and the self-powered soft robot). The development of various task types (e.g., grasping, adhesive, driving or swimming, and sensing functions) and mechanism realization forms of the underwater soft robot are described based on this article.
Rescue of follicle development after oocyte-induced ovary dysfunction and infertility in a model of POI
Authors: Sheikh Sairah , Lo Belinda K. M. , Kaune Heidy , Bansal Jassimran , Deleva Anna , Williams Suzannah A.
Published: August 2023

The mechanisms and aetiology underlying the development of premature ovarian insufficiency (POI) are poorly understood. However, the oocyte clearly has a role as demonstrated by the Double Mutant (DM) mouse model where ovarian dysfunction (6 weeks) is followed by POI (3 months) due to oocyte-specific deletion of complex and hybrid N- and O-glycans. The ovaries of DM mice contain more primary follicles (3a stage) accompanied by fewer developing follicles, indicating a block in follicle development. To investigate this block, we first analysed early follicle development in postnatal (8-day), pre-pubertal (3-week) and post-pubertal (6-week and 3-month) DM (C1galt1F/FMgat1F/F:ZP3Cre) and Control (C1galt1F/FMgat1F/F) mice. Second, we investigated if transplantation of DM ovaries into a “normal” endocrine environment would restore follicle development. Third, we determined if replacing DM ovarian somatic cells would rescue development of DM oocytes. At 3-week, DM primary 3a follicles contain large oocytes accompanied by early development of a second GC layer and increased GC proliferation. At 6-week, DM primary 3a follicles contain abnormally large oocytes, accompanied with decreased GC proliferation. Transplantation of DM ovaries into a ‘normal’ endocrine environment did not restore normal follicle development. However, replacing somatic cells by generating reaggregated ovaries (ROs) did enable follicle development to progress and thus highlighted intra-ovarian factors were responsible for the onset of POI in DM females. Thus, these studies demonstrate oocyte-initiated altered communication between GCs and oocytes results in abnormal primary follicles which fail to progress and leads to POI.
Reactivity of ethyl nitrosoacrylate toward pyrrole, indole and pyrrolo[3,2-c]carbazole: an experimental and theoretical study
Authors: Benzi Alice , Lopes Susana M. M. , Nunes Sandra C. C. , Giorgi Gianluca , Bianchi Lara , Tavani Cinzia , Pais Alberto A. C. C. , Petrillo Giovanni , Pinho e Melo Teresa M. V. D.
Published: August 2023

Nitrosoalkenes react with 8-methyl-1,6-dihydropyrrolo[3,2-c]carbazole to give both 2- and 3-alkylated products via hetero-Diels-Alder reaction followed by the cycloadduct ring-opening. Quantum chemical calculations, at DFT level of theory, were carried out to investigate the regioselectivity of the cycloaddition of ethyl nitrosoacrylate with 1,6-dihydropyrrolo[3,2-c]carbazoles as well as with pyrrole and indole, allowing a more comprehensive analysis of the reactivity pattern of nitrosoalkenes with five-membered heterocycles. Furthermore, theoretical calculations confirmed that ethyl nitrosoacrylate reacts with these heterocycles via a LUMOheterodiene-HOMOdienophile controlled cycloaddition. The reactivity of one of the oxime-functionalized 1,6-dihydropyrrolo[3,2-c]carbazole was explored and a new hexahydropyrido[4′,3':4,5]pyrrolo[3,2-c]carbazole system was obtained in high yield via a one-pot, two-step procedure.
Targeting Pseudomonas aeruginosa quorum sensing with sodium salicylate modulates immune responses in vitro and in vivo
Authors: Gerner Erik , Giraldo-Osorno Paula Milena , Johansson Loo Anna , Firdaus Rininta , Ben Amara Heithem , Werthén Maria , Palmquist Anders , Thomsen Peter , Omar Omar , Almqvist Sofia , Trobos Margarita
Published: August 2023

Introduction

Chronic infections are a major clinical challenge in hard-to-heal wounds and implanted devices. Pseudomonas aeruginosa is a common causative pathogen that produces numerous virulence factors. Due to the increasing problem of antibiotic resistance, new alternative treatment strategies are needed. Quorum sensing (QS) is a bacterial communication system that regulates virulence and dampens inflammation, promoting bacterial survival. QS inhibition is a potent strategy to reduce bacterial virulence and alleviate the negative impact on host immune response.

Aim

This study investigates how secreted factors from P. aeruginosa PAO1, cultured in the presence or absence of the QS inhibitor sodium salicylate (NaSa), influence host immune response.

Material and methods

In vitro, THP-1 macrophages and neutrophil-like HL-60 cells were used. In vivo, discs of titanium were implanted in a subcutaneous rat model with local administration of P. aeruginosa culture supernatants. The host immune response to virulence factors contained in culture supernatants (+/-NaSa) was characterized through cell viability, migration, phagocytosis, gene expression, cytokine secretion, and histology.

Results

In vitro, P. aeruginosa supernatants from NaSa-containing cultures significantly increased THP-1 phagocytosis and HL-60 cell migration compared with untreated supernatants (-NaSa). Stimulation with NaSa-treated supernatants in vivo resulted in: (i) significantly increased immune cell infiltration and cell attachment to titanium discs; (ii) increased gene expression of IL-8, IL-10, ARG1, and iNOS, and (iii) increased GRO-α protein secretion and decreased IL-1β, IL-6, and IL-1α secretion, as compared with untreated supernatants.

Conclusion

In conclusion, treating P. aeruginosa with NaSa reduces the production of virulence factors and modulates major immune events, such as promoting phagocytosis and cell migration, and decreasing the secretion of several pro-inflammatory cytokines.
Clinical characteristics of bloodstream infections in adult patients with solid tumours and a nomogram for mortality prediction: a 5-year case-controlled retrospective study in a tertiary-level hospital
Authors: Xue Lijuan , Zhu Ying , Zong Mingxi , Jiao Panpan , Fu Jianguo , Liang Xian-Ming , Zhan Juan
Published: August 2023

Background

Bloodstream infections (BSIs) are one of the leading causes of death in cancer patients. Nevertheless, the risk factors of BSIs in solid tumors have rarely been ascertained adequately.

Methods

We conducted a single-center case-controlled retrospective study from 2017 to 2021 among adults with solid tumors in a tertiary-level hospital. The BSIs and control group were matched by the propensity score matching method. We found independent risk factors of occurrence and death of BSIs using univariate and multivariate regression analysis. Additionally, a nomogram was constructed to predict the risk of mortality in BSIs.

Results

Of 602 patients with solid tumors in the study period, 186 had BSIs and 416 had non-BSIs. The incidence of BSIs was 2.0/1,000 admissions (206/102,704), and the 30-day mortality rate was 18.8% (35/186). Compared to the control group, the BSIs had longer hospital stays (24.5 days vs. 20.0 days), and higher frequency complicating with organ failure (10.5% vs. 2.4%), nephropathy (19.6% vs. 3.8%), comorbidities≥3 (35.5% vs. 20.0%), and liver-biliary-pancreatic infections (15.6% vs. 5.3%) (all P<0.001). Among the 186 patients with BSIs, 35 died within 30 days after BSIs. Gram-negative bacteria were the most frequent microorganisms (124/192, 64.6%). Liver cancer, organ failure, a high level of lactate dehydrogenase and septic shock were the independent hazardous factors for death of BSIs. What’s more, a nomogram was constructed to predict the 30-day survival rate of BSIs, which was proved to have good accuracy (AUC: 0.854; 95% confidence interval: 0.785~0923) and consistency.

Conclusion

Being aware of the risk factors of BSIs redounds to take preventive measures to reduce the incidence and death of BSIs.
Predicting 7-day unplanned readmission in elderly patients with coronary heart disease using machine learning
Authors: Song Xuewu , Tong Yitong , Luo Yi , Chang Huan , Gao Guangjie , Dong Ziyi , Wu Xingwei , Tong Rongsheng
Published: August 2023

Background

Short-term unplanned readmission is always neglected, especially for elderly patients with coronary heart disease (CHD). However, tools to predict unplanned readmission are lacking. This study aimed to establish the most effective predictive model for the unplanned 7-day readmission in elderly CHD patients using machine learning (ML) algorithms.

Methods

The detailed clinical data of elderly CHD patients were collected retrospectively. Five ML algorithms, including extreme gradient boosting (XGB), random forest, multilayer perceptron, categorical boosting, and logistic regression, were used to establish predictive models. We used the area under the receiver operating characteristic curve (AUC), accuracy, precision, recall, the F1 value, the Brier score, the area under the precision-recall curve (AUPRC), and the calibration curve to evaluate the performance of ML models. The SHapley Additive exPlanations (SHAP) value was used to interpret the best model.

Results

The final study included 834 elderly CHD patients, whose average age was 73.5 ± 8.4 years, among whom 426 (51.08%) were men and 139 had 7-day unplanned readmissions. The XGB model had the best performance, exhibiting the highest AUC (0.9729), accuracy (0.9173), F1 value (0.9134), and AUPRC (0.9766). The Brier score of the XGB model was 0.08. The calibration curve of the XGB model showed good performance. The SHAP method showed that fracture, hypertension, length of stay, aspirin, and D-dimer were the most important indicators for the risk of 7-day unplanned readmissions. The top 10 variables were used to build a compact XGB, which also showed good predictive performance.

Conclusions

In this study, five ML algorithms were used to predict 7-day unplanned readmissions in elderly patients with CHD. The XGB model had the best predictive performance and potential clinical application perspective.
Clinical implication of ticagrelor monotherapy in patients with small vessel coronary artery disease: results from the TICO randomized trial
Authors: Cho Jae Young , Joo Donghyeon , Yun Kyeong Ho , Kim Byeong-Keuk , Hong Myeong-Ki , Jang Yangsoo , Oh Seok Kyu
Published: August 2023

Background

The aim of this study was to evaluate the efficacy and safety of ticagrelor monotherapy in patients with small vessel disease compared with ticagrelor-based DAPT within the Ticagrelor Monotherapy after 3 Months in the Patients Treated with New Generation Sirolimus Eluting Stent for Acute Coronary Syndrome (TICO) trial population.

Methods

Reference vessel diameter ≤2.5 mm was considered as small vessel disease. We conducted a comparison of the incidence of target lesion failure (TLF) and Bleeding Academic Research Consortium (BARC) type 3 or 5 bleeding. TLF was defined as a composite of cardiac death, target lesion myocardial infarction, stent thrombosis, and target lesion revascularization.

Results

652 patients among 3,056 TICO population (21.3%) had small vessel disease. Patients with small vessel disease showed a higher rate of TLF compared to those without small vessel disease (2.9% vs. 1.0%, log-rank p < 0.001). The presence of small vessel disease emerged as an independent predictor for 1-year TLF (HR 2.84, 95% CI 1.54–5.25), while it did not show a significant association with bleeding complications. The 12-month TLF rate was 1.6% for ticagrelor monotherapy after 3-month DAPT, and 4.2% for ticagrelor-based 12-month DAPT (p = 0.059) in patients with small vessel disease (HR 0.38, 95% CI 0.14–1.04, p for interaction = 0.261). The incidence of BARC type 3 or 5 bleeding rate 2.5% for ticagrelor monotherapy after 3-month DAPT, and 5.6% for ticagrelor-based 12-month DAPT (p = 0.052) in patients with small vessel disease (HR 0.44, 95% CI 0.19–1.01, p for interaction = 0.322). In the 3-month landmark analysis, ticagrelor monotherapy significantly reduced BARC type 3 or 5 bleeding in patients with small vessel disease (HR 0.09, 95% CI 0.01–0.69, log-rank p = 0.005) while demonstrating a similar incidence of TLF compared to ticagrelor based 12-month DAPT during the 3–12 months period.

Conclusions

There are no significant interactions between the antiplatelet strategy regarding the 12-month incidence of ischemic and bleeding complications. Ticagrelor monotherapy demonstrated a reduction in bleeding complications after a 3-month period of DAPT without increasing the rate of TLF, when compared to ticagrelor-based 12-month DAPT, specifically in patients with small vessel disease.

Clinical Trial Registration: www.ClinicalTrials.gov, identifier, NCT02494895.
Platelet-rich fibrin improves repair and regeneration of damaged endometrium in rats
Authors: Mao Lele , Wang XiaoXue , Sun Yu , Yang Mukun , Chen Xing , Cui Lei , Bai Wenpei
Published: August 2023

Purpose

Intrauterine adhesion (IUA) is the most common cause of uterine infertility. This study aims to evaluate whether platelet-rich fibrin (PRF) treatment can stimulate damaged endometrium regeneration in rats.

Methods

First, hematoxylin and eosin (HE) staining, scanning and transmission electron microscopy, and ELISAs were used to evaluate the microstructure of PRF. Then, mechanical damage was used to establish an IUA rat model. A total of 40 SD female rats were randomized to three groups: PRF transplantation group, IUA group, and sham group. Rats were sacrificed at 3, 7, and 14 days and uteruses were obtained for further analysis. Finally, functional and histological recovery of the damaged endometrium was analyzed by pregnancy test, HE staining, Masson’s staining, and immunohistochemistry.

Results

PRF has two distinct zones, platelets and fibrin zone. Long and narrow fibrin fibers interconnected with each other and formed a three-dimensional, flexible, and elastic structure; platelet aggregates were trapped in fibrin fibers, and each platelet is associated with several fibrin fibers. PRF exudates promoted endometrial stromal cell proliferation and migration in vitro. PRF transplantation was beneficial for maintaining uterine structure, promoting endometrial luminal epithelium and endometrial gland regeneration, and decreasing fibrotic areas in vivo.

Conclusion

Intrauterine administration of PRF was demonstrated to be effective in preventing IUA and stimulating damaged endometrium regeneration in rats. This study not only provided a promising method for its potential in endometrial regeneration in women who suffer from uterine infertility but also may prevent IUA after intrauterine surgery in clinical cases.
Implication of DNA methylation during lifestyle mediated weight loss
Authors: Aurich Samantha , Müller Luise , Kovacs Peter , Keller Maria
Published: August 2023

Over the past 50 years, the number of overweight/obese people increased significantly, making obesity a global public health challenge. Apart from rare monogenic forms, obesity is a multifactorial disease, most likely resulting from a concerted interaction of genetic, epigenetic and environmental factors. Although recent studies opened new avenues in elucidating the complex genetics behind obesity, the biological mechanisms contributing to individual’s risk to become obese are not yet fully understood. Non-genetic factors such as eating behaviour or physical activity are strong contributing factors for the onset of obesity. These factors may interact with genetic predispositions most likely via epigenetic mechanisms. Epigenome-wide association studies or methylome-wide association studies are measuring DNA methylation at single CpGs across thousands of genes and capture associations to obesity phenotypes such as BMI. However, they only represent a snapshot in the complex biological network and cannot distinguish between causes and consequences. Intervention studies are therefore a suitable method to control for confounding factors and to avoid possible sources of bias. In particular, intervention studies documenting changes in obesity-associated epigenetic markers during lifestyle driven weight loss, make an important contribution to a better understanding of epigenetic reprogramming in obesity. To investigate the impact of lifestyle in obesity state specific DNA methylation, especially concerning the development of new strategies for prevention and individual therapy, we reviewed 19 most recent human intervention studies. In summary, this review highlights the huge potential of targeted interventions to alter disease-associated epigenetic patterns. However, there is an urgent need for further robust and larger studies to identify the specific DNA methylation biomarkers which influence obesity.