bluuebunny/crossref_metadata_2025 huggingface dataset:
Sulfated Polysaccharide Isolated from the Nacre of Pearl Oyster Improves Scopolamine-Induced Memory Impairment
Authors: Yamagami Hikaru , Fuji Tatsuya , Wako Mayumi , Hasegawa Yasushi
Published: March 2021

Pearl and nacre have been used in traditional medicines for treating brain dysfunctions, such as epilepsy, myopia, palpitations and convulsions. We previously showed that a pearl oyster nacre extract improves scopolamine-induced memory impairments using the Y-maze, Banes maze and object recognition tests. In this study, we aimed to isolate the memory-improving substance using ion-exchange column chromatography and reverse-phase column chromatography and elucidate the molecular mechanism underlying its memory-improving activity. The isolated substance was found to be a sulfated polysaccharide with a molecular weight of approximately 750 kDa. Monosaccharide composition analysis showed that it was rich in galactose, glucose, mannose and uronic acid. Furthermore, the mRNA expression levels of oxidative stress, inflammatory response and neuroprotective factors in the cerebral cortex were investigated. Treatment with the polysaccharide increased the expression levels of the antioxidant enzymes Cu, Zn -superoxide dismutase (SOD) and catalase and attenuated the scopolamine-mediated upregulation of the inflammatory cytokines interleukin-1 and interleukin-6. In addition, the polysaccharide suppressed the decrease in the expression levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). These findings strongly suggest that the polysaccharide in the nacre extract mediated its antiamnesic effects by preventing oxidative stress and inflammation and increasing the expression levels of BDNF and NGF.
A Simulation of the Effects of Badminton Serve Release Height
Authors: Rasmussen John , de Zee Mark
Published: March 2021

In this work, we develop and calibrate a model to represent the trajectory of a badminton shuttlecock and use it to investigate the influence of serve height in view of a new serve rule instated by the Badminton World Federation. The new rule means that all players must launch the shuttlecock below a height of 1.15 m, as opposed to the old rule whereby the required launch height was under the rib cage of the server. The model is based on a forward dynamics model of ballistic trajectory with drag, and it is calibrated with experimental data. The experiments also served to determine the actual influence of the new rule on the shuttlecock launch position. The model is used in a Monte Carlo simulation to determine the statistical influence of the new serve rules on the player’s ability to perform good serves; i.e., serves with little opportunity for the receiver to attack. We conclude that, for the female player in question, serving below a height of 1.15 m makes it marginally more difficult to perform excellent serves. We also conclude that there might be alternative launch positions that would be less likely to produce the best serves but could be exploited as a tactical option.
A Regional Geography Approach to Understanding the Environmental Changes as a Consequence of the COVID-19 Lockdown in Highly Populated Spanish Cities
Authors: Rodrigo-Comino Jesús , Senciales-González José María
Published: March 2021

Spain has been highly impacted by the COVID-19 pandemic, which is reflected at different scales. From an economic point of view, lockdowns and the reduction of activities have damaged the country (e.g., complete lockdown from March 13 to June 21, 2020). However, it is not clear if the associated environmental impacts could be observed in 2020. Currently, studies on the effects of the lockdown (e.g., decrease in economic activities, transport and social communication) on specific parameters related to climate change, such as air temperature or air pollution, due to a drastic decrease in human activities are rare. They are focused on specific cities and short periods of time. Therefore, the main goal of our research will be to assess the records of air temperature and air quality during the whole of 2020 compared to references from previous years (30 years for air temperature and 10 for air quality). We paid attention to the possible effects of the reduction of activities (e.g., tourism and transport) in March, April and May and the different restrictions of each lockdown in Spain. To achieve this goal, five urban climate stations with long-term time series within the most populated cities of Spain were analyzed (Barcelona, Madrid, Málaga, Sevilla and Valencia). We conclude that it is possible to affirm that the impacts of the COVID-19 pandemic on the atmospheric conditions in 2020 are not clear and not strictly focused on the lockdown or reduction of activities in these urban areas. No evidence of a reduction in the annual air temperature was found, and only a minimum reduction of rates of pollutants was registered in the highly populated cities of Spain. Therefore, it is worth confirming that these changes could be affected by other factors, but on a global scale and not directly due to the COVID-19 pandemic. Considering recent studies on the relationships between air pollutants, temperature and the spreadability of COVID-19, green policies must be further imposed in urban cities, since temperatures do not stop increasing year by year.
Analysis of the Experimental Integration of Thermoelectric Generators in Photovoltaic–Thermal Hybrid Panels
Authors: Pintanel Mª Teresa , Martínez-Gracia Amaya , Galindo Mª Pilar , Bayod-Rújula Ángel A. , Uche Javier , Tejero Juan A. , del Amo Alejandro
Published: March 2021

Photovoltaic–thermal panels (PVT) have been widely studied in the last years and have proved to be a technically viable and profitable solution. This work analyses the integration of a set of thermoelectric generators (TEG) inside these panels in order to obtain additional power. The thermoelectric material takes advantage of the temperature gap between the hottest part of the system, the output flow from the collector, and the cold water feeding the solar system. An experimental test bench with a PVT having integrated TEGs and the same PVT in parallel without TEGs was mounted to compare both devices. The corresponding CFD simulation was also carried out to better understand the temperature map in the arrangement. Both experimental and computational results show that the manufacture of the panel with integrated TEGs should be carefully studied before becoming a commercial product. They also gave some guidelines for the improvement of the prototype in this integrated product.
Conventional Selection of Mechanical Fasteners for Flat Belts
Authors: Konecki Karol , Wojtkowiak Dominik , Talaśka Krzysztof , Kołodziej Andrzej , Domek Grzegorz
Published: March 2021

Due to the variety of materials used for flat belts of belt conveyors and the further development of material engineering in relation to these belts, the methods of their connection become an increasingly problematic issue. The belts can be connected mainly in three ways: vulcanized (weldable or heat-weldable), glued or mechanically. The latter method is one of the simplest and most universal in terms of the material variety of belts; however, there are many design variations of mechanical fasteners, and each of them has a certain advantage in a narrow group of properties, e.g., the thickness spectrum of a conveyor belt, the minimum diameter of a drive roller or the range of transferable longitudinal loads. The objective of this paper is to analyze the design solutions of commercial mechanical fasteners used mainly for flat rubber-fabric, composite or plastic belts. To fulfill this goal, a preliminary analysis of the stress distribution for an exemplary solid mechanical fastener was carried out in two cases: during ramp-up and during circulating around the roll, followed by a detailed review of commercial solutions available on the market. In addition to determining the current state of knowledge and technology and determining the state of ignorance, special algorithm and design maps have been created, thanks to which the process of selecting the right mechanical fastening will be easier. The overview includes several tables with detailed information on individual connection properties. Additionally, several design aspects were derived, within which individual mechanical connections may differ. This is to enable the generation of customized solutions in the future by proposing an appropriate mathematical model, on the basis of which it will be possible to generate optimal design properties for a given application.
Writing for Emotional Impact in Film and Video Games: Lessons in Character Development, Realism, and Interactivity from the Alien Media Franchise
Authors: Thomas Christian
Published: March 2021

This article compares Ridley Scott’s film Alien (1979) with Creative Assembly’s video game Alien: Isolation (2014), which is based on Scott’s film. Guidance for academics who teach creative writing—as well as for working screenwriters and video game narrative designers—emerges in the comparison, particularly with regard to the importance of developing strong yet vulnerable main characters who put themselves in danger in order to protect other characters with whom they have meaningful relationships. Examples from other media, including Ira Levin’s Rosemary’s Baby (1967), James Cameron’s Aliens (1986), Telltale Games’ The Walking Dead (2012), and Naughty Dog’s The Last of Us (2013), are also discussed as they relate to larger principles involved in crafting sympathetic characters, realistic settings, and compelling gameplay for media within the horror and sci-fi genres.
Targeted Double-Stranded cDNA Sequencing-Based Phase Analysis to Identify Compound Heterozygous Mutations and Differential Allelic Expression
Authors: Ura Hiroki , Togi Sumihito , Niida Yo
Published: March 2021

There are two combinations of heterozygous mutation, i.e., in trans, which carries mutations on different alleles, and in cis, which carries mutations on the same allele. Because only in trans compound heterozygous mutations have been implicated in autosomal recessive diseases, it is important to distinguish them for clinical diagnosis. However, conventional phase analysis is limited because of the large target size of genomic DNA. Here, we performed a genetic analysis on a patient with Wilson disease, and we detected two heterozygous mutations chr13:51958362;G>GG (NM_000053.4:c.2304dup r.2304dup p.Met769HisfsTer26) and chr13:51964900;C>T (NM_000053.4:c.1841G>A r.1841g>a p.Gly614Asp) in the causative gene ATP7B. The distance between the two mutations was 6.5 kb in genomic DNA but 464 bp in mRNA. Targeted double-stranded cDNA sequencing-based phase analysis was performed using direct adapter ligation library preparation and paired-end sequencing, and we elucidated they are in trans compound heterozygous mutations. Trio analysis showed that the mutation (chr13:51964900;C>T) derived from the father and the other mutation from the mother, validating that the mutations are in trans composition. Furthermore, targeted double-stranded cDNA sequencing-based phase analysis detected the differential allelic expression, suggesting that the mutation (chr13:51958362;G>GG) caused downregulation of expression by nonsense-mediated mRNA decay. Our results indicate that targeted double-stranded cDNA sequencing-based phase analysis is useful for determining compound heterozygous mutations and confers information on allelic expression.
Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis
Authors: Jeong Jieun , Kim Jae-Seok , Lee Junghyeon , Seo Yu , Yi Eugene , Kim Kristine
Published: March 2021

Gonorrhea is one of the most common, but still hidden and insidious, sexually transmitted diseases caused by Neisseria gonorrhoeae (gonococci). However, the diagnosis and treatment of gonorrhea are hampered by antigenic variability among gonococci, the lack of acquired immunity, and antimicrobial resistance. Further, strains resistant to cephalosporins, including ceftriaxone, the last line of defense, represent a growing threat, which prompted us to develop gonococci-specific diagnostic antibodies with broad-spectrum binding to gonococci strains to generate gonorrhea-detecting reagents. This study reports the identification of gonococci antibodies via bio-panning on gonococci cells using scFv-phage libraries. Reformatting the lead scFv-phage Clones 1 and 4 to a multivalent scFv1-Fc-scFv4 maxibody increased the sensitivity by up to 20-fold compared to the single scFv-Fc (maxibody) alone. Moreover, the multivalent maxibody showed broader cross-reactivity with clinical isolates and the ceftriaxone antibiotic-resistant World Health Organization (WHO) reference strain L. In contrast, the selected antibodies in the scFv-phage, maxibody, and multivalent maxibody did not bind to N. sicca, N. meningitides, and N. lactamica, suggesting the clinical and pharmaceutical diagnostic value of these selected antibodies for gonorrheal infections. The present study illustrates the advantages and potential application of multivalent maxibodies to develop rapid and sensitive diagnostic reagents for infectious diseases and cancer.
Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli
Authors: Casas-Arrojo Virginia , Decara Juan , de los Ángeles Arrojo-Agudo María , Pérez-Manríquez Claudia , Abdala-Díaz Roberto
Published: March 2021

Porphyridium cruentum is a unicellular microalga that can synthesize and secrete to the culture medium-high amounts of polysaccharides. In this study, the immunomodulatory, cytotoxic effect and antioxidant activity of the sulfated polysaccharides (PcSPs) were determinate. The PcSPs were precipitated with 2% Cetylpyridinium bromide hydrate and ethanol and purified by dialysis. The extract was lyophilized for its characterization by Fourier transform-Infrared (FT-IR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The antioxidant activity of PcSPs were examined with assay 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and compared with that of the biomass, observing significant differences between the results obtained from the PcSPs and biomass. To determine their ability to induce cytokine production Tumor Necrosis Factor alpha (TNF-α) and interleukina-6 (IL-6), the immunomodulatory activity of the PcSPs has been evaluated. In the mouse macrophage cell line (RAW 264.7), PcSPs are potent inducers of IL-6 cytokines but mainly of TNF-α. The cytotoxic capacity of PcSPs was measured by the MTT colorimetric assay in colorectal carcinoma (HTC-116), human leukemia (U-937 and HL-60), breast cancer (MCF-7), lung cancer (NCI-H460) and human gingival fibroblasts (HGF-1) cell lines. The IC50 value of 2311.20 µg mL−1, 1676.74 µg mL−1, 1089.63 µg mL−1, 5498.14 µg mL−1 and 2861.49 µg mL−1 respectively in the tumor lines and 5022.55 µg mL−1 in gingival fibroblasts were obtained. Our study suggested that PcSPs from P. cruentum have a moderate immunomodulatory and cytotoxic effect. The results obtained indicate that the polysaccharides from P. cruentum are potent inducers of IL-6 cytokines and, most importantly, of TNF-α. PcSPs showed no evidence of antigenic activity or hypersensitivity when administered intraperitoneally in mice. Furthermore, the in vivo study revealed an improvement of local inflammatory response against stress in the peritoneum. These findings suggest that the PcSPs from P. cruentum might have potential as a valuable ingredient in nutraceutical products.
Use of Empirical Mode Decomposition in ERP Analysis to Classify Familial Risk and Diagnostic Outcomes for Autism Spectrum Disorder
Authors: Abou-Abbas Lina , van Noordt Stefon , Desjardins James A. , Cichonski Mike , Elsabbagh Mayada
Published: March 2021

Event-related potentials (ERPs) activated by faces and gaze processing are found in individuals with autism spectrum disorder (ASD) in the early stages of their development and may serve as a putative biomarker to supplement behavioral diagnosis. We present a novel approach to the classification of visual ERPs collected from 6-month-old infants using intrinsic mode functions (IMFs) derived from empirical mode decomposition (EMD). Selected features were used as inputs to two machine learning methods (support vector machines and k-nearest neighbors (k-NN)) using nested cross validation. Different runs were executed for the modelling and classification of the participants in the control and high-risk (HR) groups and the classification of diagnosis outcome within the high-risk group: HR-ASD and HR-noASD. The highest accuracy in the classification of familial risk was 88.44%, achieved using a support vector machine (SVM). A maximum accuracy of 74.00% for classifying infants at risk who go on to develop ASD vs. those who do not was achieved through k-NN. IMF-based extracted features were highly effective in classifying infants by risk status, but less effective by diagnostic outcome. Advanced signal analysis of ERPs integrated with machine learning may be considered a first step toward the development of an early biomarker for ASD.
Extracellular Vesicle-Associated Tissue Factor Activity in Prostate Cancer Patients with Disseminated Intravascular Coagulation
Authors: Hell Lena , Däullary Thomas , Burghart Vanessa , Mauracher Lisa-Marie , Grilz Ella , Moser Bernhard , Kramer Gero , Schmid Johannes A. , Ay Cihan , Pabinger Ingrid , Thaler Johannes
Published: March 2021

Patients with advanced prostate cancer may develop fulminant disseminated intravascular coagulation (DIC). Circulating extracellular vesicles (EVs)-exposing tissue factor (TF), the initiator of the coagulation cascade, may play an important role. We included 7 prostate cancer patients with DIC, 10 age- and stage-matched cancer controls without DIC, and 10 age-matched healthy male individuals. EV-TF activity was highly elevated in prostate cancer patients with DIC (11.40 pg/mL; range: 4.34–27.06) compared with prostate cancer patients without DIC (0.09 pg/mL; range: 0.00–0.30, p = 0.001) and healthy controls (0.18 pg/mL; range: 0.09–0.54; p = 0.001). Only EVs from patients with DIC reduced fibrin clot formation time of pooled plasma in a TF-dependent manner. Next, we performed in vitro co-culture experiments including EVs derived from a prostate cancer cell line with high (DU145) and low (LNCaP) TF expression, peripheral blood mononuclear cells (PBMCs), and platelets. Co-incubation of DU145 EVs with PBMCs and platelets significantly increased EV-TF activity in conditioned medium and induced TF activity on monocytes. No such effects were seen in co-culture experiments with LNCaP EVs. In conclusion, the findings indicate that elevated EV-TF activity plays a role in the development of prostate-cancer-related DIC and may result from interactions between tumor-derived EVs, monocytes, and platelets.
Detection of Microsatellite Instability: State of the Art and Future Applications in Circulating Tumour DNA (ctDNA)
Authors: Gilson Pauline , Merlin Jean-Louis , Harlé Alexandre
Published: March 2021

Microsatellite instability (MSI) is a molecular scar resulting from a defective mismatch repair system (dMMR) and associated with various malignancies. MSI tumours are characterized by the accumulation of mutations throughout the genome and particularly clustered in highly repetitive microsatellite (MS) regions. MSI/dMMR status is routinely assessed in solid tumours for the initial screening of Lynch syndrome, the evaluation of cancer prognosis, and treatment decision-making. Currently, pentaplex PCR-based methods and MMR immunohistochemistry on tumour tissue samples are the standard diagnostic methods for MSI/dMMR. Other tissue methods such as next-generation sequencing or real-time PCR-based systems have emerged and represent viable alternatives to standard MSI testing in specific settings. The evolution of the standard molecular techniques has offered the opportunity to extend MSI determination to liquid biopsy based on the analysis of cell-free DNA (cfDNA) in plasma. This review aims at synthetizing the standard and emerging techniques used on tumour tissue samples for MSI/dMMR determination. We also provide insights into the MSI molecular techniques compatible with liquid biopsy and the potential clinical consequences for patients with solid cancers.
Rare Germline Pathogenic Variants Identified by Multigene Panel Testing and the Risk of Aggressive Prostate Cancer
Authors: Nguyen-Dumont Tú , Dowty James G. , MacInnis Robert J. , Steen Jason A. , Riaz Moeen , Dugué Pierre-Antoine , Renault Anne-Laure , Hammet Fleur , Mahmoodi Maryam , Theys Derrick , Tsimiklis Helen , Severi Gianluca , Bolton Damien , Lacaze Paul , Sebra Robert , Schadt Eric , McNeil John , Giles Graham G. , Milne Roger L. , Southey Melissa C.
Published: March 2021

While gene panel sequencing is becoming widely used for cancer risk prediction, its clinical utility with respect to predicting aggressive prostate cancer (PrCa) is limited by our current understanding of the genetic risk factors associated with predisposition to this potentially lethal disease phenotype. This study included 837 men diagnosed with aggressive PrCa and 7261 controls (unaffected men and men who did not meet criteria for aggressive PrCa). Rare germline pathogenic variants (including likely pathogenic variants) were identified by targeted sequencing of 26 known or putative cancer predisposition genes. We found that 85 (10%) men with aggressive PrCa and 265 (4%) controls carried a pathogenic variant (p < 0.0001). Aggressive PrCa odds ratios (ORs) were estimated using unconditional logistic regression. Increased risk of aggressive PrCa (OR (95% confidence interval)) was identified for pathogenic variants in BRCA2 (5.8 (2.7–12.4)), BRCA1 (5.5 (1.8–16.6)), and ATM (3.8 (1.6–9.1)). Our study provides further evidence that rare germline pathogenic variants in these genes are associated with increased risk of this aggressive, clinically relevant subset of PrCa. These rare genetic variants could be incorporated into risk prediction models to improve their precision to identify men at highest risk of aggressive prostate cancer and be used to identify men with newly diagnosed prostate cancer who require urgent treatment.
Ultrasound-Assisted Hydrothermal Synthesis of V2O5/Zr-SBA-15 Catalysts for Production of Ultralow Sulfur Fuel
Authors: Ramos Jesús Miguel , Wang Jin An , Flores Sergio Odin , Chen Lifang , Arellano Ulises , Noreña Luis Enrique , González Julio , Navarrete Juan
Published: March 2021

This work reports the results of the ultrasound-assisted hydrothermal synthesis of two sets of V2O5 dispersed on SBA-15 and Zr doped SBA-15 catalysts used for the oxidation of dibenzothiophene (DBT) in a model diesel via the combination of oxidation, catalysis, and extraction technical route. These catalysts contained Lewis acidity as major and Brønsted acidity as minor. The amount of acidity varied with the content of vanadia and zirconium doping. It was found that DBT conversion is very sensitive to the Lewis acidity. DBT conversion increased by increasing the vanadium content and correlated well with the amount of surface Lewis acidity. Under the optimal experimental condition (Reaction temperature: 60 °C, reaction time 40 min, catalyst concentration: 1 g/L oil; H2O2/DBT mole ratio = 10), the 30% V2O5/SBA-15 and 30% V2O5/Zr-SBA-15 catalysts could convert more than 99% of DBT. Two reaction pathways of DBT oxidation involving vanadia surface structure, Lewis acidity, and peroxometallic complexes were proposed. When the vanadia loading V2O5 ≤ 10 wt%, the oxidative desulfurization (ODS) went through the Pathway I; in the catalysts with moderate vanadia content (V2O5 = 20–30 wt%), ODS proceeded via the Pathways II or/and the Pathway I.
Plasmonic Photocatalysts
Authors: Kowalska Ewa
Published: March 2021

Plasmonic photocatalysts, i [...]
Extinction Effect of Gold Nanocatalysts on Photocatalytic Activities under Plasmonic Excitation
Authors: Kim Donghee , Kim Youngsoo
Published: March 2021

Plasmonic nanoparticles (NPs), particularly Au NPs, are potential candidates for photocatalysts because of their unique optical properties. The size of Au NPs plays a crucial role in effective light absorption, which is an important factor in photocatalytic reactions. Although Au NP-based photocatalysts have garnered significant researched interest, the size effect of Au NPs on a photocatalytic reaction has not been sufficiently studied. We characterized the effect of size on the photocatalytic activity of Au NPs of different sizes. We found that the absorption cross-section of the Au NPs gradually increased as the size of the Au NPs increased. However, the reaction rate for each size of NP was inversely proportional to the absorption cross-section. Based on the simulation results, we found that larger Au NPs have a higher scattering factor than that of smaller Au NPs. Consequently, the scattering effect of Au NPs hinders effective light absorption, resulting in slower reaction kinetics. These findings can contribute to the rational design of high-efficiency plasmonic photocatalysts.
Electrochemical Detection of Prostate Cancer Biomarker PCA3 Using Specific RNA-Based Aptamer Labelled with Ferrocene
Authors: Nabok Alexei , Abu-Ali Hisham , Takita Sarra , Smith David P.
Published: March 2021

This paper reports on a feasibility study of electrochemical in-vitro detection of prostate cancer biomarker PCA3 (prostate cancer antigen 3) in direct assay with specific RNA aptamer labelled with a redox group (ferrocene) and immobilized on a screen-printed gold electrode surface. The cyclic voltammograms and electrochemical impedance spectroscopy methods yield encouraging results on the detection of PCA3 in a range of concentrations from 1 μg/mL down to 0.1 ng/mL in buffer solutions. Both anodic and cathodic current values in cyclic voltammograms measurements and charge transfer resistance values in electrochemical impedance spectroscopy experiments correlate with the PCA3 concentration in the sample. Kinetics studies of the binding of the PCA3 to our aptamer demonstrated high specificity of the reaction with a characteristic affinity constant of approximately 4·10−10 molar. The results of this work provide a background for the future development of novel, highly sensitive and cost-effective diagnostic methodologies for prostate cancer detection.
Challenges in the Computational Modeling of the Protein Structure—Activity Relationship
Authors: Del Río Gabriel
Published: March 2021

Living organisms are composed of biopolymers (proteins, nucleic acids, carbohydrates and lipid polymers) that are used to keep or transmit information relevant to the state of these organisms at any given time. In these processes, proteins play a central role by displaying different activities required to keep or transmit this information. In this review, I present the current knowledge about the protein sequence–structure–activity relationship and the basis for modeling this relationship. Three representative predictors relevant to the modeling of this relationship are summarized to highlight areas that require further improvement and development. I will describe how a basic understanding of this relationship is fundamental in the development of new methods to design proteins, which represents an area of multiple applications in the areas of health and biotechnology.
Autonomous Underwater Robot Fuzzy Motion Control System with Parametric Uncertainties
Authors: Zhilenkov Anton , Chernyi Sergei , Firsov Andrey
Published: March 2021

The paper describes the design of a fuzzy motion control system of an autonomous underwater vehicle. A mathematical model of the underwater vehicle is synthesized. A fuzzy regulator for controlling the depth of immersion autonomous underwater vehicle is designed. The quality of control for step control, harmonic control, as well as various types of exogenous disturbances, is investigated. The comparison of the functioning quality of the designed fuzzy controller with the proportional–derivative controller is made. It is shown that the designed fuzzy controller provides a higher quality of control compared to the proportional–derivative controller. The proposed fuzzy controller provides high-quality control of the plant under uncertainties.
Advanced Techniques in the Percutaneous Ablation of Liver Tumours
Authors: Hui Terrence CH , Kwan Justin , Pua Uei
Published: March 2021

Percutaneous ablation is an accepted treatment modality for primary hepatocellular carcinoma (HCC) and liver metastases. The goal of curative ablation is to cause the necrosis of all tumour cells with an adequate margin, akin to surgical resection, while minimising local damage to non-target tissue. Aside from the ablative modality, the proceduralist must decide the most appropriate imaging modality for visualising the tumour and monitoring the ablation zone. The proceduralist may also employ protective measures to minimise injury to non-target organs. This review article discusses the important considerations an interventionalist needs to consider when performing the percutaneous ablation of liver tumours. It covers the different ablative modalities, image guidance, and protective techniques, with an emphasis on new and advanced ablative modalities and adjunctive techniques to optimise results and achieve satisfactory ablation margins.
Analysis of Autoantibodies against Promyelocytic Leukemia Nuclear Body Components and Biochemical Parameters in Sera of Patients with Primary Biliary Cholangitis
Authors: Bauer Alicja , Habior Andrzej , Wieszczy Paulina , Gawel Damian
Published: March 2021

Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by immune-mediated destruction of intrahepatic bile ducts and the presence of specific antibodies. The aim of the study was to examine the diagnostic significance of antibodies against promyelocytic leukemia nuclear body (PML NB) components such as Sp100, Sp140, and PML in a cohort of PBC patients and compare the results with biochemical and histological parameters. Serum samples were collected from 93 PBC patients. Anti-Sp100 and anti-PML antibodies were assessed using commercially available kits, anti-Sp140 using developed “in-house” ELISA test. Anti-Sp140, anti-Sp100, and anti-PML antibodies were present in 25 (27%), 37 (40%), and 29 (31%) PBC patients, respectively. Anti-PML NB positive patients also showed increased concentration of bilirubin and alkaline phosphatase (p < 0.05). In the group with the presence of at least two types of these antibodies, more frequent deaths or transplantations were observed. A correlation between the presence of antibodies and histological grade (OR = 2.55 p = 0.039) was established. Patients with bilirubin > 1.1 mg/dL at the time of diagnosis had a significantly shorter time of survival than patients with bilirubin ≤ 1.1 mg/dL (HR 5.7; 95% C.I., 2.7, 12.3; p < 0.001). Our data confirm very high specificity of anti-PML NB antibodies, which can expand the laboratory diagnostic capabilities of PBC. We found an association between positive reactivity of autoantibodies directed against components of PML nuclear bodies and higher concentrations of bilirubin and alkaline phosphatase, but the main prognostic marker of survival remains serum bilirubin.
Double Slot Antenna for Microwave Thermal Ablation to Treat Bone Tumors: Modeling and Experimental Evaluation
Authors: Trujillo-Romero Citlalli Jessica , Leija-Salas Lorenzo , Vera-Hernández Arturo , Rico-Martínez Genaro , Gutiérrez-Martínez Josefina
Published: March 2021

According to statistics of the American Cancer Society, the number of young people diagnosed with bone tumors is growing. Surgery and radiotherapy are the common treatments, however they have several side effects which affect the patient’s life. Therefore, a cheaper and less side-effect therapy called thermal ablation has been explored. The goal of this paper is to measure the therapeutic temperatures and the viability of a double slot antenna designed to treat bone tissue by microwave ablation. The antenna (at an operating frequency of 2.45 GHz) was designed, modeled, constructed, and experimentally evaluated. The finite element method was used to predict the antenna performance by means of 2D axisymmetric models. The modeling parameters were used to build the antenna. The experimental evaluation shows that the antenna behavior is repeatable and the standing wave ratio (SWR) was around 1.5–1.8. Temperatures around 60–100 °C were achieved over the bone tissue. The antenna insertion modifies the antenna performance. An insertion lower than 3.5 cm is not recommended because the convection effects modified the tissue temperature. The thermal patterns showed a heat focus near to the slots, which makes it possible for use in the treatment of small tumors.
On the Accurate Numerical Analysis of the Propagation Through Dielectric Frequency-Selective Surfaces Using a Vectorial Modal Method
Authors: Coves Ángela , San-Blas Ángel A.
Published: March 2021

In this work, we focus on the numerical analysis of the propagation of plane-waves in one-dimensional periodic lossy dielectric media, which constitute the building block of dielectric frequency-selective surfaces (DFSSs). To this end, a full-vectorial modal method was used, in which discontinuities of some components of the electromagnetic fields have to be evaluated, and we propose a numerical improvement in the evaluation of some integrals appearing in the developed formulation. Some confusion may exist in the evaluation of the cited integrals due to the discontinuous nature of the dielectric function and its transverse gradient. Therefore, some considerations are given in order to solve these integrals accurately for the general case of a relative dielectric permittivity function defined as a sum of lossy dielectric slabs. We particularize our study to a dielectric frequency-selective surface (DFSS), for which the periodic dielectric medium can be defined as constant functions inside an homogeneous region, whose contours define the discontinuities. Thus, the relative dielectric permittivity can be expressed in terms of the Heaviside or step function. In this way, the above-mentioned integrals can be correctly evaluated in the discontinuity, obtaining good results with the employed vectorial modal method for both the propagation constant and the electromagnetic fields obtained in the periodic dielectric medium constituting the DFSS. These results are compared with those obtained with a less accurate evaluation of the cited integrals, when an approximation made by other authors is used.
A Method for Measuring the Maximum Measurable Gain of a Passive Intermodulation Chamber
Authors: Cai Zhanghua , Zhou Yantao , Liu Lie , de Paulis Francesco , Qi Yihong , Orlandi Antonio
Published: March 2021

This paper presents an approximate method that allows the calculation of the maximum measurable gain (MMG) in an anechoic chamber. This method is realized by using a low passive intermodulation (PIM) medium-gain directional antenna. By reducing the distance between the antenna and the wall of the chamber to reduce path loss, the purpose of replacing a high-gain antenna with a medium-gain antenna is achieved. The specific relationship between distance and equivalent gain is given in this paper. The measurement interval is determined by the 3 dB beamwidth of the measurement antenna to scan the whole chamber. A set of corresponding data for the residual PIM level and the MMG of the chamber can be obtained by the method of measurement outlined herein. The feasibility of this method was verified by measurements in two PIM measurement chambers.
Improved YOLOv3 Network for Insulator Detection in Aerial Images with Diverse Background Interference
Authors: Liu Chuanyang , Wu Yiquan , Liu Jingjing , Sun Zuo
Published: March 2021

Automatic inspection of insulators from high-voltage transmission lines is of paramount importance to the safety and reliable operation of the power grid. Due to different size insulators and the complex background of aerial images, it is a difficult task to recognize insulators in aerial views. Most of the traditional image processing methods and machine learning methods cannot achieve sufficient performance for insulator detection when diverse background interference is present. In this study, a deep learning method—based on You Only Look Once (YOLO)—will be proposed, capable of detecting insulators from aerial images with complex backgrounds. Firstly, aerial images with common aerial scenes were collected by Unmanned Aerial Vehicle (UAV), and a novel insulator dataset was constructed. Secondly, to enhance feature reuse and propagation, on the basis of YOLOv3 and Dense-Blocks, the YOLOv3-dense network was utilized for insulator detection. To improve detection accuracy for different sized insulators, a structure of multiscale feature fusion was adapted to the YOLOv3-dense network. To obtain abundant semantic information of upper and lower layers, multilevel feature mapping modules were employed across the YOLOv3-dense network. Finally, the YOLOv3-dense network and compared networks were trained and tested on the testing set. The average precision of YOLOv3-dense, YOLOv3, and YOLOv2 were 94.47%, 90.31%, and 83.43%, respectively. Experimental results and analysis validate the claim that the proposed YOLOv3-dense network achieves good performance in the detection of different size insulators amid diverse background interference.
An Open Circuit Voltage Model Fusion Method for State of Charge Estimation of Lithium-Ion Batteries
Authors: Yu Quanqing , Wan Changjiang , Li Junfu , E Lixin , Zhang Xin , Huang Yonghe , Liu Tao
Published: March 2021

The mapping between open circuit voltage (OCV) and state of charge (SOC) is critical to the lithium-ion battery management system (BMS) for electric vehicles. In order to solve the poor accuracy in the local SOC range of most OCV models, an OCV model fusion method for SOC estimation is proposed. According to the characteristics of the experimental OCV–SOC curve, the method divides SOC interval (0, 100%) into several sub-intervals, and respectively fits the OCV curve segments in each sub-interval to obtain a corresponding number of OCV sub-models with local high precision. After that, the OCV sub-models are fused through the continuous weight function to obtain fusional OCV model. Regarding the OCV curve obtained from low-current OCV test as the criterion, the fusional OCV models of LiNiMnCoO2 (NMC) and LiFePO4 (LFP) are compared separately with the conventional OCV models. The comparison shows great fitting accuracy of the fusional OCV model. Furthermore, the adaptive cubature Kalman filter (ACKF) is utilized to estimate SOC and capacity under a dynamic stress test (DST) at different temperatures. The experimental results show that the fusional OCV model can effectively track the performance of the OCV–SOC curve model.
Observation of the Main Natural Parameters Influencing the Formation of Gas Hydrates
Authors: Gambelli Alberto Maria , Tinivella Umberta , Giovannetti Rita , Castellani Beatrice , Giustiniani Michela , Rossi Andrea , Zannotti Marco , Rossi Federico
Published: March 2021

Chemical composition in seawater of marine sediments, as well as the physical properties and chemical composition of soils, influence the phase behavior of natural gas hydrate by disturbing the hydrogen bond network in the water-rich phase before hydrate formation. In this article, some marine sediments samples, collected in National Antarctic Museum in Trieste, were analyzed and properties such as pH, conductivity, salinity, and concentration of main elements of water present in the sediments are reported. The results, obtained by inductively coupled plasma-mass spectrometry (ICP-MS) and ion chromatography (IC) analysis, show that the more abundant cation is sodium and, present in smaller quantities, but not negligible, are calcium, potassium, and magnesium, while the more abundant anion is chloride and sulfate is also appreciable. These results were successively used to determine the thermodynamic parameters and the effect on salinity of water on hydrates’ formation. Then, hydrate formation was experimentally tested using a small-scale apparatus, in the presence of two different porous media: a pure silica sand and a silica-based natural sand, coming from the Mediterranean seafloor. The results proved how the presence of further compounds, rather than silicon, as well as the heterogeneous grainsize and porosity, made this sand a weak thermodynamic and a strong kinetic inhibitor for the hydrate formation process.
Time-Varying Convergences of Environmental Footprint Levels between European Countries
Authors: Yıldırım Durmuş Çağrı , Yıldırım Seda , Erdoğan Seyfettin , Demirtaş Işıl , Couto Gualter , Castanho Rui Alexandre
Published: March 2021

This study proposes the time-varying nonlinear panel unit root test to investigate the convergence of ecological foot prints between the EU and candidate countries. Sixteen European countries (such as Albania, Austria, Belgium, Denmark, France, Germany, Greece, Italy, Luxembourg, Netherlands, Poland, Portugal, Romania, Spain, Sweden and Turkey) and analysis periods are selected according to data availability. This study proposes a cross-sectional Panel KSS with Fourier to test the convergence of the ecological footprints. Then, we combine this methodology with the rolling window method to take into account the time-varying stationarity of series. This study evaluated sub-components of ecological footprints separately and provided more comprehensive findings for the ecological footprint. According to empirical findings, this study proves that convergence or divergence does not show continuity over time. On the other side, this study points out the presence of divergence draws attention when considering the properties of the sub-components in general. As a result, this study shows that international policies by EU countries are generally accepted as successful to reduce ecological footprint, but these are not sufficient as expected. In this point, it is suggested to keep national policies to support international policies in the long term.
Reduced-Impact Logging Maintain High Moss Diversity in Temperate Forests
Authors: Hernández-Rodríguez Enrique , Escalera-Vázquez Luis H. , García-Ávila Deneb , Montoro Girona Miguel , Mendoza Eduardo
Published: March 2021

Forestry harvesting represents an important economic activity around the world. Habitat degradation due to forest harvesting contributes to biodiversity loss; therefore, it is necessary to implement logging management aimed at reducing its impact. Forest management by reduce-impact logging (RIL) involves cutting trees following regulations focused on diminishing the impact on biodiversity by following harvesting plans based on forestry inventories and participation of trained workers. In Mexico, RIL is applied mainly in temperate habitats and its effectiveness has been assessed based on vascular plants. In this study, we analyzed the diversity and community structure of terrestrial and epiphytic mosses in managed (sites number = 3) and conserved (sites number = 3) sites in the temperate forest of Sierra Juárez, Oaxaca, Mexico. Likewise, we evaluated the potential function of mosses as indicators of habitat degradation. Environmental variables were also quantified at local (canopy coverage, altitude, daily temperature, and light) and regional (total annual rainfall, orientation, and slope) scales to evaluate potential relationships with the community and species diversity. We documented 70 mosses species with a diversity (alfa, beta) and community structure similar between managed and conserved sites. For terrestrial mosses, we found marginal differences in their communities, likely related to species coverture variation in managed sites. The diversity and community structure epiphytic mosses were not statistically different in managed and conserved sites. Only the daily variation in light intensity was positively related to the variation of alpha diversity of epiphytic mosses. The species Dicranum sumichrastii Duby and Leptodontium viticulosoides (P. Beauv.) Wijk & Margad. can be considered as ecological indicators for conserved and managed sites, respectively, likely due to their relationship with light and humidity conditions. Our results suggest that that forest management by RIL could be considered as a promising tool to balance timber production and moss diversity.
Regeneration Dynamics and Development of Seedlings in Sessile Oak Forests in Relation to the Light Availability and Competing Vegetation
Authors: Kanjevac Branko , Krstić Milun , Babić Violeta , Govedar Zoran
Published: March 2021

Natural regeneration of sessile oak forests is a complex process whose final outcome is influenced by numerous factors. The initial phase of development of sessile oak seedlings stands out as the most critical period in the process of natural regeneration of sessile oak forests. As the available light and competition from the accompanying woody species and ground vegetation are some of the main predictors of the success of sessile oak forest regeneration, this paper presents the results of studying the influence of these factors on the regeneration dynamics and development of sessile oak seedlings. The research was conducted in sessile oak forests in northeastern Serbia in the period from 2015 to 2020. At the end of the growing season each year, the following data were collected on 180 smaller sampling plots of 1 m2 in different conditions of canopy openness: the number, height, and root collar diameter of sessile oak seedlings. Also, the percent cover of competing woody species and ground vegetation was estimated on these sample plots. The obtained results indicated that the success of sessile oak forest regeneration largely depends on the initial number of sessile oak seedlings and silvicultural treatments during the rejuvenation period, which determine the microclimatic conditions in the stand and control the competing vegetation. They also indicate that with an increase in the available light, the impact of the competing vegetation on the dynamics of regeneration and development of sessile oak seedlings is less pronounced. Accordingly, as young sessile oak seedlings need a large amount of light for survival and development, it is necessary to increase the available amount of light intensively in a short period of time (six–eight years) by reducing canopy openness and thus providing optimal conditions in which sessile oak seedlings can gain an advantage over the competing vegetation.