bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS CEMARAN LOGAM BERAT ARSEN, TIMBAL, DAN MERKURI PADA MAKANAN DI WILAYAH KOTA SURABAYA DAN KABUPATEN SIDOARJO JAWA TIMUR
Authors: Dewi Eva Rosdiana
Published: June 2023

Makanan merupakan sumber energi untuk menunjang aktivitas manusia. Selain memberikan dampak positif, makanan dapat menimbulkan dampak negatif yaitu penyakit yang disebabkan oleh makanan (food borne disease). Kota Surabaya dan Kabupaten Sidoarjo merupakan kota/kabupaten di Jawa Timur yang memiliki wilayah pesisir dan industri yang cukup banyak. Kontribusi limbah industri lebih besar mencemari lingkungan dengan logam berat. Logam berat yang terakumulasi dalam tubuh jika melebihi batas maksimum dapat menyebabkan terganggunya fungsi jaringan, organ, kerusakan otak sampai kematian. Penelitian bertujuan untuk mengetahui kadar logam berat arsen, timbal, dan merkuri serta batas aman kandungan yang dapat dikonsumsi manusia. Metode penelitian ini adalah deskriptif dengan menganalisis hasil uji cemaran logam berat pada makanan. Sampel makanan terdiri dari 16 sampel untuk tiap uji cemaran logam berat arsen, timbal, dan merkuri. Hasil dari penelitian ini tidak ada makanan yang mengandung logam berat arsen. Makanan yang mengandung timbal adalah terasi dengan kadar timbal sebanyak 0,005-0,01 ppm. Makanan yang mengandung merkuri adalah kerang dan keong sawah dengan kadar merkuri sebanyak 0,005 ppm. Hasil pengujian makanan yang mengandung cemaran logam berat dapat berasal dari bahan baku, habitat, dan adanya pembuangan limbah industri di daerah aliran sungai. Kesimpulan penelitian adalah semua sampel makanan negatif mengandung arsen namun positif mengandung timbal dan merkuri di bawah batas aman kandungan logam berat, sehingga masih aman untuk dikonsumsi manusia. Saran yang dapat diberikan adalah pemerintah perlu melakukan kajian lebih lanjut terhadap makanan yang mengandung logam berat. Selain itu, pengawasan peredaran makanan perlu partisipasi aktif masyarakat untuk dapat meminimalkan terjadinya terjadinya pencemaran logam berat pada makanan.
HUBUNGAN PEKERJAAN MANUAL MATERIAL HANDLING DENGAN KELUHAN MUSCULOSKELETAL DISORDERS PADA PENGANTAR GALON
Authors: Pratiwi Arum Dian , Nurmaladewi Nurmaladewi , Nasruddin Nasruddin
Published: June 2023

Pekerjaan manual material handling yang dilakukan oleh pengantar galon dapat menimbulkan beberapa keluhan pada tubuh seperti bahu, lengan, punggung, pinggang, pinggul dan pergelangan tangan yang disebut sebagai keluhan musculoskeletal disorders. Prevalensi musculoskeletal disorders berdasarkan hasil survei Health Safety Executive di Britania Raya menunjukkan sebanyak 507.000 pekerja mengalami musculoskeletal disorders tahun 2017, 469.000 pekerja tahun 2018, dan 498.000 pekerja tahun 2019. Prevalensi musculoskeletal disorders di Indonesia dan Sulawesi Tenggara berdasarkan hasil Riset Kesehatan Dasar tahun 2018 yakni 7,30% dan 5,63%. Adapun prevalensi penyakit sistem otot dan jaringan di Kota Kendari berdasarkan Profil Kesehatan Kota Kendari tahun 2017 yakni 7,66 %, 9,00% tahun 2018, dan 9,35% tahun 2019. Tujuan dari penelitian ini adalah untuk mengetahui hubungan pekerjaan manual material handling dengan keluhan musculoskeletal disorders pada pengantar galon di Kota Kendari. Jenis penelitian ini merupakan penelitian observasional analitik dengan menggunakan pendekatan  cross sectional. Uji statistik yang digunakan yakni spearman rank. Populasi dalam penelitian ini adalah seluruh pengantar galon yang berjumlah 356 orang dengan jumlah sampel yang diambil sebanyak 127 orang. Hasil penelitian ini menunjukkan bahwa ada hubungan antara waktu, sikap tubuh dan risiko pekerjaan manual material handling dengan keluhan musculoskeletal disorders dan tidak terdapat hubungan antara kondisi kerja dengan keluhan musculoskeletal disorders. Pemilik usaha galon dan pengantar galon hendaknya membatasi waktu mengantar galon, menghindari sikap tubuh membungkuk, memperhatikan kondisi kerja atau depot, serta membatasi jumlah galon yang diangkat.
ANALISIS KEMAMPUAN LILI PARIS (CHLOROPHYTUM COMOSUM VARIEGATUM) DALAM MENYERAP FORMALIN DI RUANGAN
Authors: Wulandari Ranimas Ayu , Ma’rufi Isa , Ellyke Ellyke
Published: June 2023

Paparan gas formaldehyde dalam ruangan dapat menyebabkan gangguan kesehatan seperti iritasi, vertigo, dan bersifat karsinogenik, tumorogenik, sitotoksik. Sumber formaldehyde dalam ruangan yaitu cat tembok, cat kuku, dan alat makan melamin. Jumlah yang signifikan dari melamin dan formaldehyde dilepaskan dari pembelian melaware di pasar Denmark untuk stimulasi makanan pada suhu 70oC dan 95oC. Penelitian ini bertujuan untuk menganalisis kemampuan tanaman lili paris (Chlorophytum comosum V.) dalam menyerap polutan gas formaldehyde sebesar 0,6 mg/m3 di ruangan. Metode penelitian ini adalah True Experimental Design dengan bentuk penelitian pretest-postest control group design. Penelitian dilakukan terhadap dua kelompok acak, kemudian dilakukan pengukuran untuk mengetahui kondisi awal sebelum diberi perlakuan dan pengukuran setelah diberikan perlakuan. Pengulangan dilakukan sebanyak 24 kali berdasarkan rumus RAL (Rancangan Acak Lengkap). Pengukuran gas formaldehyde dilakukan dengan menggunakan alat UNI-T A25F PM 2,5 Meter. Analisis data dalam penelitian ini diolah menggunakan uji One Way Anova. Hasil dari penelitian ini diketahui bahwa container yang tidak diberi tanaman lili paris tidak terjadi penurunan konsentrasi formaldehyde, sedangkan container yang diberi tanaman lili paris terjadi penurunan konsentrasi formaldehyde. Berdasarkan hasil analisis, penurunan konsentrasi formaldehyde tertinggi terdapat pada container dengan jumlah tanaman lili paris terbanyak yaitu berisi 3 tanaman atau container P3 dibandingkan dengan container P1 dan P2 yang hanya berisi satu dan dua pot tanaman lili paris. Satu pot tanaman lili paris mampu menurunkan konsentrasi formaldehyde sebesar 0,4 ppm. Kesimpulannya tanaman lili paris mampu menurunkan polutan gas formaldehyde di ruangan dan cocok diletakkan dalam ruangan dengan konsentrasi formaldehyde lebih dari 0,06 ppm.
HUBUNGAN INTENSITAS PENCAHAYAAN DAN JARAK PENGLIHATAN DENGAN KELUHAN KELELAHAN MATA OPERATOR JAHIT
Authors: Nurhayati Itsna , Atmojo Tutug Bolet , Sari Yulia
Published: June 2023

Lingkungan kerja memiliki faktor dan bahaya yang dapat menyebabkan ketidaknyamanan pekerja dalam melakukan pekerjaanya. Salah satu faktor yang terdapat di dalam lingkungan kerja adalah intensitas pencahayaan. Intensitas pencahayaan bergantung sifat dan jenis pekerjaan yang dilakukan. Pekerjaan menjahit merupakan jenis pekerjaan yang membutuhkan ketelitian serta menggunakan  penglihatan jarak dekat, sehingga diperlukan intensitas pencahayaan yang sesuai. Ketidaksesuaian besarnya intensitas cahaya yang diterima oleh pekerja serta jarak penglihatan antara mata dengan objek kerja yang berisiko dapat menyebabkan munculnya keluhan kelelahan mata. Penelitian ini bertujuan untuk menganalisis hubungan intensitas pencahayaan dan jarak penglihatan dengan keluhan kelelahan mata operator jahit di PT X. Merupakan penelitian analitik dengan pendekatan cross sectional. Sampel berjumlah 72 pekerja yang diambil menggunakan teknik pengambilan sampel secara simple random sampling. Hasil penelitian menunjukkan p value = 0,021 untuk hubungan intensitas pencahayaan dengan keluhan kelelahan mata dan p value = 0,004 untuk hubungan jarak penglihatan dengan keluhan kelelahan mata. Dalam penelitian ini, jarak penglihatan memiliki pengaruh yang paling besar terhadap terjadinya keluhan kelelahan mata. Pekerja yang memiliki jarak penglihatan yang berisiko, memiliki kemungkinan untuk mengalami keluhan kelelahan mata sebesar 11,9 kali dibandingkan pekerja dengan jarak penglihatan yang tidak berisiko. Kesimpulan yang dapat diperoleh yaitu ada hubungan antara intensitas pencahayaan dan jarak penglihatan dengan keluhan kelelahan mata. Semakin besar ketidaksesuaian intensitas pencahayaan di tempat kerja, maka semakin tinggi keluhan kelelahan mata. Semakin berisiko jarak penglihatan pekerja, semakin tinggi keluhan kelelahan mata. Saran yang dapat diberikan yaitu dengan melakukan pengaturan jarak lampu dan melakukan istirahat mata.
FAKTOR YANG MEMPENGARUHI GREEN TOBACCO SICKNESS PADA PEKERJA ANAK DI PERKEBUNAN TEMBAKAU
Authors: Kartika Nabila Zandra , Ririanty Mury , Indrayani Reny
Published: June 2023

Kandungan nikotin daun tembakau basah yang terserap permukaan kulit anak dapat menimbulkan penyakit Green Tobacco Sickness (GTS). Secara simultan penyakit GTS dipengaruhi oleh faktor perilaku, lingkungan, dan pelayanan kesehatan. Tujuan penelitian untuk mengkaji faktor yang mempengaruhi kejadian GTS pada pekerja anak di perkebunan tembakau. Penelitian ini menggunakan metode narrative literature review. Narrative literature review berfokus pada pembuktian kerangka pemikiran yang berhubungan dengan rumusan masalah penelitian. Pencarian referensi penelitian menggunakan 3 database yaitu Google Scholar, Science Direct, dan ResearchGate. Artikel terpilih yang memenuhi kriteria inklusi sebanyak 13 artikel dengan menggunakan 5 kata kunci dalam pencarian referensi. Hasil penelitian menunjukkan beberapa faktor yang berhubungan dengan kejadian GTS pada pekerja anak adalah kepatuhan penggunaan alat pelindung diri, lama paparan pekerja anak dengan daun tembakau, pengetahuan dan persepsi orang tua terhadap penyakit GTS pada anak, serta sosialisasi mengenai penyakit GTS oleh industri tembakau, kepala kelompok tani ataupun tenaga kesehatan setempat.
FAKTOR YANG BERHUBUNGAN DENGAN DEPRESI PADA REMAJA OVERWEIGHT-OBESITAS DI KABUPATEN JOMBANG
Authors: Annahar Cici Nia , Hendrati Lucia Yovita
Published: June 2023

Remaja overweight-obesitas rentan mengalami penyakit tidak menular, salah satunya adalah depresi. Peningkatan jumlah remaja overweight-obesitas menimbulkan peningkatan kejadian depresi. Hal ini dapat mempengaruhi penurunan kualitas hidup remaja. Tujuan penelitian ini adalah untuk menganalisis faktor yang berhubungan dengan depresi pada remaja overweight-obesitas. Penelitian ini merupakan observasional analitik dengan rancang bangun cross sectional. Responden berjumlah 43 remaja usia 15 – 19 tahun dengan overweight-obesitas di Kabupaten Jombang. Variabel bebas penelitian ini adalah jenis kelamin, binge eating, dan persepsi citra tubuh. Sedangkan variabel terikatnya adalah depresi. Peneliti mengumpulkan data menggunakan angket online. Penelitian ini dilakukan pada bulan Maret – Mei 2021. Sampel dipilih dengan accidental sampling. Analisis data menggunakan uji korelasi Rank Spearman. Hasil penelitian ini menunjukkan bahwa sebagian besar remaja overweight-obesitas tidak mengalami depresi sebesar 69,8%, sebagian besar tidak mengalami binge eating sebesar 62,8%, dan sebagian besar responden memiliki ketidakpuasan tubuh sedang sebesar 41,9%. tidak ada hubungan yang signifikan antara jenis kelamin, binge eating dengan kejadian depresi pada remaja overweight-obesitas. Sedangkan variabel persepsi citra tubuh memiliki hubungan yang siginifikan dengan kejadian depresi pada remaja overweight-obesitas. Depresi pada remaja overweight-obesitas paling banyak berjenis kelamin perempuan, memiliki ketidakpuasan citra tubuh sedang, dan tidak mengalami binge eating. Remaja dengan overweight-obesitas disarankan untuk menerapkan pola hidup sehat, mengubah stigma penurunan berat badan menjadi peningkatan perilaku hidup sehat, meningkatkan kepercayaan dan kepuasan diri dan melakukan konsultasi gizi dan psikologis apabila diperlukan.
ANALISIS PROGRAM PENCEGAHAN COVID-19 DI KAMPUS X JAKARTA
Authors: Listyawati Rohanita Winny , Lestari Putri Winda
Published: June 2023

Sebuah penyakit baru telah menggemparkan seluruh dunia sejak akhir tahun 2019. Penyakit ini dikenal dengan Coronavirus Disease 2019 (Covid-19). Tujuan penelitian untuk menganalisis program pencegahan Covid-19 di Kampus X Jakarta. Penelitian menggunakan metode deskripstif kualitatif. Objek penelitian adalah program pencegahan Covid-19 (prosedur dan kebijakan perusahaan di masa Pandemi Covid-19, program edukasi, sarana dan prasarana, aturan perjalanan, serta hambatan) di Kampus X Jakarta, informan sebanyak 6 orang. Data diambil dengan wawancara mendalam dan observasi lapangan. Hasil penelitian menunjukkan bahwa Kampus X sudah cukup kooperatif dalam mengikuti arahan pemerintah dengan membentuk tim untuk melaksanakan program pencegahan Covid-19. Telah ada prosedur dan kebijakan pencegahan Covid-19, program edukasi dan sarana prasarana. Namun implementasi di lapangan masih belum maksimal. Saran agar Kampus X menyediakan sarana prasarana yang belum ada, Tim Tanggap Covid-19 melakukan pengawasan secara maksimal, meningkatkan kesadaran civitas akademik dalam melakukan pencegahan Covid-19.

Kata Kunci: Covid-19, Program pencegahan, Pandemi.
HUBUNGAN HIGIENE SANITASI DENGAN KEBERADAAN E.coli PADA MINUMAN OLAHAN DI KENDARI BEACH
Authors: Fithria Fithria , Yasnani Yasnani , Alhajar Hayatun Nufusi
Published: June 2023

Minuman tidak hanya patut bergizi dan menarik, namun pula patut bebas berdasarkan zat berbahaya misalnya bahan kimia, mikroorganisme, dan kontaminan lainnya Penyebab terkontaminasisinya makanan oleh Escherichia coli acapkalikali terjadi lantaran sanitasi pengelolaan makanannya yang tidak memenuhi syarat kesehatan. Salah satu jenis jajanan yang relatif berisiko merupakan berdasarkan jenis minuman. Penelitian ini bertujuan mengetahui hubungan higiene dan sanitasi dengan keberadaan bakteri Escherichia coli pada minuman olahan di Kendari Beach Kota Kendari tahun 2022. Metode penelitian ini dengan penelitian kuantitatif dengan peneratan analitik observasional. Adapun desain dari penelitian ini termasuk kedalam penelitian cross sectional. Hasil penelitian ini menunjukkan dari 32 sampel minuman olahan di Kendari Beach Kota Kendari yang diuji terdapat 26 (81,2%) sampel yang ditemukan bakteri Escherichia coli dan 6 (18,8%) sampel minuman olahan tidak ditemukan Escherichia   coli. Ada hubungan higiene penjamah minuman olahan dengan keberadaan bakteri Escherichia coli pada minuman olahan di Kendari Beach Kota Kendari tahun 2022 dengan p-value 0,023. Tidak ada hubungan sanitasi tempat minuman olahan dengan keberadaan bakteri Escherichia coli pada minuman olahan di Kendari Beach Kota Kendari tahun 2022 dengan p-value 0,083. Tidak ada hubungan sanitasi tempat minuman olahan dengan keberadaan bakteri Escherichia coli pada minuman olahan di Kendari Beach Kota Kendari tahun 2022 dengan p-value 0,072.

Kata kunci: Minuman Olahan, Escherichia coli, Higiene Sanitasi
STRES KERJA PADA PEGAWAI SEKRETARIAT BADAN PENDIDIKAN DAN PELATIHAN KEUANGAN KEMENTERIAN KEUANGAN DI MASA PANDEMI COVID-19
Authors: S Galuh Arieliyna Anggraini , Mustakim Mustakim
Published: June 2023

Menurut studi Occupational Health and Safety Authority (HSE), terdapat 595.000 kasus stres dan depresi terkait pekerjaan pada tahun 2017/2018 dengan prevalensi 1.800 kasus per 100.000 karyawan. Menurut survei, 64% pekerja Indonesia mengatakan tingkat stres mereka meningkat dibandingkan tahun sebelumnya. Penelitian ini merupakan penelitian kuantitatif dengan desain penelitian cross-sectional yang bertujuan untuk mengidentifikasi faktor-faktor yang berhubungan dengan kejadian stres terkait pekerjaan pada pegawai Sekretariat BPPK Kementerian Keuangan selama pandemi COVID-19. Dalam penelitian ini jumlah sampel adalah 117 responden. Hasil uji statistik menunjukkan bahwa 59% karyawan mengalami stres kerja berat. Hasil penelitian menunjukkan bahwa ada hubungan antara beban kerja karyawan dengan stres kerja (p=0,000) dan ada hubungan yang signifikan antara dukungan sosial dengan stres kerja (p=0,034). Karyawan didorong untuk bersikap positif secara emosional terhadap beban kerja yang diberikan dan pimpinan diharapkan dapat memantau karyawan dengan melakukan evaluasi atau survei atau menyelenggarakan kegiatan penggalangan dana secara online untuk meningkatkan kerjasama.
PENGARUH PENGGUNAAN VIDEO ANIMASI DAN THREAD TWITTER TERHADAP PENGETAHUAN GIZI SAAT PANDEMI COVID-19
Authors: Khoirunnisa Safira , Kurniasari Ratih , Sefrina Linda Riski
Published: June 2023

Konsumsi makanan bergizi seperti buah dan sayur adalah salah satu cara yang dapat membantu seseorang untuk mencegah infeksi virus Covid-19. Namun, tingkat konsumi buah dan sayur masyarakat di Indonesia masih rendah. Salah satu faktor penyebab dari rendahnya konsumsi tersebut adalah kurangnya pemahaman masyarkaat terkait manfaat mengkonsumsi buah dan sayur. Pemberian edukasi gizi mengenai buah dan sayur dapat menjadi salah satu cara untuk meningkatkan kesadaran masyarkaat untuk konsumsi buah dan sayur sesuai dengan kebutuhan. Edukasi gizi selama pandemi Covid-19 yang mengutamakan jaga jarak membutuhkan media yang modern dan inovatif, seperti video animasi dan thread Twitter. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian edukasi gizi menggunakan video animasi dan thread Twitter buah dan sayur terhadap peningkatan pengetahuan buah dan sayur pada usia 20-24 tahun. Penelitian ini merupakan penelitian eksperimen semu dengan desain penelitian pretest-posttest control group yang dilakukan satu kali pada bulan April 2021 di Kota Bekasi menggunakan 30 responden. Responden pada kelompok intervensi diberikan media thread Twitter dan kelompok kontrol diberikan media video animasi. Data pengetahuan sebelum dan sesudah intervensi diambil mengunakan kuesioner kemudian dianalisis menggunakan paired sample t-test dan independent t-test. Hasil penelitian menunjukkan bahwa pengetahuan responden pada kedua kelompok meningkat setelah mendapatkan edukasi gizi (p = 0,03). Kesimpulan dari hasil penelitian ini adalah edukasi gizi menggunakan media video animasi dan thread Twitter mampu meningkatkan pengetahuan buah dan sayur sehingga dapat dijadikan alternatif media edukasi gizi di masa pandemi Covid-19
EMPLOYEE STRIKE IN THE PERSPECTIVE OF HUMAN RESOURCES AND TRANSFORMATIVE LEADERSHIP
Authors: Wijaya Sonny , Herachwati Nuri , Pramesti Gebrina Ayu
Published: June 2023

One of the human resource problems in the Company is the employee strike. The strike that resulted in the layoff employees which occurred in 2017 until now in 2022 is still a polemic. The purpose of this study is to determine the suitability of strikes carried out by workers / workers and the process of layoffs by companies with the provisions regulated in the Manpower Law and for knowing the causes of strikes from the aspects of the quality of human resources and transformational leadership. This type of research is qualitative research using a literature approach. The technique for collecting study materials is to use library research techniques, case studies and prescriptive techniques of analytical methods. The results showed that strikes carried out by workers/laborers were illegal according to laws and regulations. The act of dismissal is carried out in accordance with the provisions of the legislation.
Advancements and Insights in Exosome-Based Therapies for Wound Healing: A Comprehensive Systematic Review (2018-June 2023)
Authors: Sousa Patrícia , Lopes Bruna , Sousa Ana Catarina , Moreira Alícia , Coelho André , Alvites Rui , Alves Nuno , Geuna Stefano , Maurício Ana Colette
Published: June 2023

Exosomes have shown promising potential as a therapeutic approach for wound healing. Nevertheless, the translation from experimental studies to commercially available treatments is still lacking. To assess the current state of research in this field, a systematic review was performed to examine the methodological heterogeneity among studies conducted over the past five years. Additionally, the review analyzed the suitability of animal models used and their relevance to human medicine. A PubMed search was performed for english-language, full-text available papers published from 2018 to June 2023, focusing on exosomes derived from mammalian sources and their application in wound healing, particularly those involving in vivo assays. Out of 531 results, 148 papers were selected for analysis. The findings revealed significant variability in terms of cell sources and types, biomaterials, and administration routes under investigation, indicating the need for further research in this field. Additionally, a comparative examination encompassing diverse cellular origins, types, administration pathways, or biomaterials is imperative. Furthermore, the predominance of rodent-based animal models raises concerns, as there have been limited advancements towards more complex in vivo models and scale-up assays. These constraints underscore the substantial efforts that remain necessary before attaining commercially viable and extensively applicable therapeutic approaches using exosomes.
Essential Oil From Curcuma longa Leaves: Using Nanotechnology To Make a Promising Eco-Friendly Bio-Based Pesticide From a Medicinal Plant Waste
Authors: Flexa-Ribeiro Bianca , Garcia Jr. Manoel Daltro Nunes , Silva Ana Carolina de Jesus , Carvalho José Carlos Tavares , Rocha Leandro Machado , Faustino Silvia Maria Mathes , Fernandes Caio Pinho , Da Silva Hellen Freitas , Machado Francisco Paiva , Hage-Melim Lorane Izabel da Silva , Souto Raimundo Nonato Picanço , Botas Gisele da Silva , Cruz Rodrigo Alves Soares
Published: June 2023

Nano-emulsions of essential oils (EO) and their chemical constituents are promising raw materials for the ecological control of Tribolium castaneum. Curcuma longa Linn is a plant known for the properties of its rhizome which is used in food, health and hygiene products. Its leaves are by-products with no commercial value, but with unexplored bioactive volatile constituents. This study aims to evaluate the repellency of nano-emulsions containing the EO from leaves of C. longa or its three main chemical constituents against T. castaneum. The representative mixture of EO extracted in four different months showed p-cymene (26.0%), 1,8-cineole (15.1%) and terpinolene (15.5%) as major compounds. Nano-emulsions of EO (HLB 16.7), terpinolene (HLB 15.0), 1,8-cineole (HLB15.0) and p-cymene (HLB 15.0) were repellent at concentrations of 11 μg/cm2 (EO, terpinolene and p-cymene) and 1.1 μg/cm2 (1,8-cineole). The EO nano-emulsion droplet size increased linearly over time, remaining below 300 nm for 35 days and below 600 nm for 80 days. The EO nano-emulsion proved to be a green alternative to synthetic pesticides, as it was safe against the bioindicator Chlorella vulgaris. Terpinolene, 1,8-cineol and p-cymene were able to inhibit the enzyme telomerase from T. castaneum in an in silico assay, which may explain the repellency of these samples. This study provides ideas for the utilization of EO from leaves of C. longa as raw material of new environmentally friendly plant-derived nanobiopesticides.
Fabrication of Gold-Graphitic Carbon Nitride Composite Electrode for the Detection of Pimobendan
Authors: Luk Hsiang-Ning , Chang Xu-Jia , Wu Ren-Jang
Published: June 2023

In this study, the preparation of the composite 3% Au/graphite-C3N4 (3% Au/g-C3N4) sensing material was prepared by doping 3% Au onto the surface of graphitic carbonitride (C3N4) for detection pimobendan. By the way of sensing materials characterization, the X-ray diffraction analysis (XRD) and transmission electron Microscope (TEM) were performed. The cyclic voltammetry (CV) was used to measure the concentration and redox properties of pimobendan. It can be seen that when the voltage value is 0.05 V, and a reduction peak was appeared and the pimobendan concentration (0 to 55 μM) has a relationship with the reduction current value. At low concentrations of pimobendan concentration from 0.0 to 0.8 μM, the linearity R2 = 0.9642 with a detection limit of 0.28 μM. A possible pimobendan sensing mechanism on 3% Au/g-C3N4 was proposed.
Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between
Authors: Oliel Matan , Mastai Yitzhak
Published: June 2023

Chiral interactions play a crucial role in both chemistry and biology. Understanding the behavior of chiral molecules and their interactions with other molecules is essential, and chiral interactions in solutions are particularly important for studying chiral compounds. Chirality influences the physical and chemical properties of molecules, including solubility, reactivity, and biological activity. In this work, we used Isothermal Titration Calorimetry (ITC), a powerful technique for studying molecular interactions, including chiral interactions in solutions. We conducted a series of ITC measurements to investigate the heat of dilution and the heat of racemization of several amino acids (Asn, His, Ser, Ala, Met, and Phe). We also performed ITC measurements under different solute concentrations and temperatures to examine the effects of these parameters on chiral interactions, as well as the heat of dilution and racemization. The results of our measurements indicated that the heat of dilution, specifically the interactions between the solvent (water) and solute (chiral molecules), had a significant impact compared to the chiral interactions in the solution, which were found to be negligible. This suggests that the interactions between chiral molecules and the solvent play a more dominant role in determining the overall behavior and properties of the system. By studying chiral interactions in solutions, we can gain valuable insights into the behavior of chiral compounds, which can have implications in various fields, including drug design, chemical synthesis, and biological processes.
Hardening of Respiratory Syncytial Virus Inclusion Bodies by Cyclopamine Proceeds through Perturbation of the Interactions between the M2-1 Protein, RNA and the P Protein
Authors: Diot Cédric , Richard Charles-Adrien , Risso-Ballester Jennifer , Martin Davy , Fix Jenna , Eléouët Jean-François , Sizun Christina , Rameix-Welti Marie-Anne , Galloux Marie
Published: June 2023

Respiratory syncytial virus (RSV) RNA synthesis takes place in cytoplasmic viral factories also called inclusion bodies (IBs), which are membrane-less organelles concentrating the viral RNA polymerase complex. IBs assembly is driven by liquid-liquid phase separation promoted by interactions between the viral nucleoprotein N and the phosphoprotein P. We recently demonstrated that cyclopamine (CPM) inhibits RSV multiplication by disorganizing and hardening IBs. Although a single mutation in the viral transcription factor M2-1 induced resistance to CPM, the mechanism of action of CPM still remains to be characterized. Here, using FRAP experiments on reconstituted pseudo-IBs both in cellula and in vitro, we first demonstrated that CPM activity depends on the presence of M2-1 together with N and P. We show that CPM impairs the competition between P and RNA binding to M2-1. As mutations on both P and M2-1 induced resistance against CPM activity, we suggest that CPM may affect the dynamics of the M2-1−P interaction, thereby affecting the relative mobility of proteins contained in RSV IBs. Overall, our results reveal that stabilizing viral protein-protein interactions is an attractive new antiviral approach. They pave the way for the rational chemical optimization of new specific anti-RSV molecules.
Towards Youth Mental Health System Reform: An Evaluation of Participatory Systems Modelling in the Australian Capital Territory
Authors: Lee Grace Yeeun , Hickie Ian Bernard , Song Yun Ju C , Huntley Sam , Ho Nicholas , Loblay Victoria , Freebairn Louise , Skinner Adam , Crosland Paul , Moore Elizabeth , Johnson Natalie , Lentern Stephanie , Brogden Josephine , Barry Erin , Vacher Catherine , Rosenberg Sebastian , Mayers Paul , Iannelli Olivia , Park Shin Ho , Occhipinti Jo-An
Published: June 2023

Australia’s mental health system is failing young people. Calls for accountability, strategic long-term policy planning, and regional leadership have been identified as solutions to guide mental health reform. Developing system dynamics models using a participatory approach (participatory systems modelling, PSM) is recognised as a useful method that can support decision-making for strategic reform. This paper reports evaluation findings of a youth mental health PSM process conducted in the Australian Capital Territory (ACT). Baseline and follow-up mixed-methods evaluation data were collected in 2022 across diverse stakeholder groups to investigate the feasibility, value, impact, and sustainability of PSM. Though youth mental health system reform was viewed as desirable and a necessity across all stakeholder groups, shared perceptions of disabling powerless was observed regarding their ability to influence current decision-making processes to improve the youth mental health system. This suggests greater accountability is required to support systemic reform in youth mental health. PSM offers promise in improving transparency and accountability of decision-making for youth mental health as exemplified in the ACT. However, more support and time are required to facilitate transformational change. Future research should investigate empowerment strategies to complement the implementation of findings from dynamic models developed through PSM, as well as the effectiveness of regional youth mental health policy decision-making supported by systems modelling.
3D Framework Carbon for High-Performance Zinc-Ion Capacitors
Authors: Kiatikajornjumroen Setthathon , Liu Xiaopeng , Lu Yinan , Deka Boruah Buddha
Published: June 2023

With the rapid and continuous advancement and deployment of advanced energy storage de-vices, there has been significant interest in aqueous capacitors that possess non-flammable properties and high safety features. Consequently, extensive research efforts have focused on investigating zinc anodes and low-cost carbonaceous cathode materials. Despite these efforts, the development of high-performance zinc-ion capacitors (ZICs) still faces challenges such as limited cycling stability and low energy densities. In this study, we present a novel approach to address these challenges. We demonstrate the utilization of a three-dimensionally (3D) grown conductive porous carbon framework cathode coupled with zinc anode cells, which exhibit exceptional sta-bility and durability in ZICs. Our experiments reveal a remarkable cycling performance, with a capacity retention of approximately 97.3% and a coulombic efficiency of nearly 100% even after 10,000 charge-discharge cycles. These findings signify significant progress in enhancing the performance of ZICs.
Triplin: Mechanistic Basis for Voltage-Gating
Authors: Colombini Marco , Liu Patrick , Dee Chase
Published: June 2023

The outer membrane of Gram-negative bacteria contains a variety of pore-forming structures collectively referred to as porins. Some of these are voltage-dependent, but weakly so, closing at high voltages. Triplin, a novel bacterial pore-former, is a three-pore structure, highly voltage dependent, with a complex gating process. The three pores close sequentially: pore 1 at positive potentials, 2 at negative and 3 at positive. A positive domain containing 14 positive charges (the voltage sensor) translocates through the membrane during the closing process and the translocation is proposed to take place by the domain entering the pore and thus blocking it resulting in the closed conformation. This mechanism of pore closure is supported by kinetic measurements that show that in the closing process the voltage sensor travels through most of the transmembrane voltage before reaching the energy barrier. Voltage-dependent blockage of the pores by polyarginine but not by a 500 fold higher concentrations of polylysine, is consistent with the model of pore closure, with the sensor consisting mainly of arginine residues, and with the presence, in each pore, of a complementary surface that serves as a binding site for the sensor.
Prediction of Tropospheric Ozone Levels from Temperature in the Urban Area of Durango, Dgo
Authors: Loera-Sánchez José Manuel , Montiel-Antuna Eusebio , Morones-Esquivel Miriam Mirelle , López-Serrano Pablito Marcelo , Briseño-Reyes Jaime , Garcia-Montiel Emily , Ramírez-Aldaba Hugo
Published: June 2023

Air pollution in urban centers depends directly on anthropogenic activities. The objective of the present study was to predict tropospheric ozone levels from temperature data in the city of Durango, Dgo. Temperature and tropospheric ozone values were obtained from 18 sampling points in the urban area of the city of Durango, of which 15 were obtained by collecting from the Ventusky service and the rest from three fixed monitoring stations established in the city. These values were interpolated in order to determine the spatial values for the entire city. A correlation analysis was performed for three different periods in the day established as follows (24:00-03:00-06:00, 09:00-12:00-15:00 and 15:00-18:00-21:00). Subsequently, a linear regression analysis was performed by time period. The results showed a higher positive correlation between ozone concentration and temperature from 15:00 hrs to 21:00 hrs; likewise, this period showed a higher goodness of fit in the ozone prediction (R2=0.99; RMSE: 1.12 ppbv). Temperature allows spatial prediction of ozone concentrations in urban areas with acceptable accuracy.
Inhibition of Topoisomerases by Metal Thiosemicarbazone Complexes
Authors: Jiang Xiaohua , Fielding Lauren A. , Davis Hunter , Carroll William , Lisic Edward C. , Deweese Joseph E.
Published: June 2023

Topoisomerases, common targets for anti-tumor therapeutics, are crucial enzymes for DNA replication, transcription, and many other aspects of DNA metabolism. The potential anti-cancer effect of thiosemicarbazones (TSC) and metal-TSC complexes has been demonstrated by targeting several biological processes, including DNA metabolism. Human topoisomerases were discovered among the molecular targets for TSCs, and metal-chelated TSCs specifically displayed significant inhibition. The processes by which metal-TSCs or TSCs inhibit topoisomerases are still being studied. In this brief review, we summarize the TSCs and metal-TSCs that inhibit various types of human topoisomerases.
New Food-Frequency Questionnaire to Estimate Vitamin K intake in a Mediterranean Population
Authors: Pinto Ezequiel , Viegas Carla , Martins Paula Ventura , Nascimento Tânia , Schurgers Leon , Simes Dina
Published: June 2023

Vitamin K is a multifunctional micronutrient essential for human health, and deficiency has been linked to multiple pathological conditions. In this study we aimed to develop and validate a new food frequency questionnaire (FFQ) to estimate total vitamin K intake, over the course of a 30-day interval, in a Portuguese, Mediterranean-based, population. We conducted a prospective study in a non-random sample of 38 healthy adult volunteers. The FFQ was designed based on a validated Portuguese FFQ used in nationally representative studies and on literature reviews, to include foods containing ≥ 5μg of vitamin K/100g and foods with a lower vitamin K content, yet commonly in-cluded in a Mediterranean diet. Vitamin K intake was estimated from 24h recalls and six days of food records. The final FFQ included 54 food items which, according to regression analyses, explains 90% of vitamin K intake. Mean differences in vitamin K intake based on food records (80±47.7 μg/day) and on FFQ (96.5±64.3 μg/day) were statistically non-significant. Further, we found a strong correlation between both methods (r= 0.7; p=0.003). Our results suggest that our new FFQ is a valid instrument to assess the last 30-days of vitamin K intake in the Portuguese Mediterranean population.
Study of Interest of Farmer Group Like Forward in The Use of Trichoderma Asperellum in Rice Cultivation in Bomba Village, Sigi District
Authors: Sayani Sayani , Sudewi Sri , Ratnawati Ratnawati , Jaya Kasman , Renaldi Renaldi , Rahim Saleh Abdul
Published: June 2023

The intensive and excessive use of chemical fertilizers and pesticides can have various impacts on soil fertility, farmers' health, and impacts on the environment. One alternative to minimize this impact is by using microorganisms, namely the fungus Trichoderma asperellum. This study aims to find out how interested or interested members of the Suka Maju Farmer Group in Bomba Village, Sigi Regency, Central Sulawesi, are in applying Trichoderma asperellum to rice cultivation land. The method used is quantitative descriptive analysis with primary data collection including observation, direct interviews, and semi-structured questionnaires that have been prepared, while secondary data is obtained from literature studies or source tracing related to research issues. Respondents in this study were all members of the Suka Maju Farmer Group, totaling 25 people. Data analysis used descriptive analysis using Microsoft Excel 2013 software. The observational variables of this study were the internal and external factors of the respondents, the level of interest of the respondents based on indicators of disinterest, lack of interest, interest, and very interest as well as the distribution of interest indicators (satisfaction, willingness, spirit, skill) with the use of T. asperellum. The results obtained were that members of the Suka Maju Farmer Group in Bomba Village had an interest of ≥ 70% in the use of T. asperellum in rice cultivation. To attract farmers' interest, counseling strategies, training, and continuous assistance from agricultural extension workers are needed, information delivery using effective and optimal media and supported by effective communication between extension farmers and related parties.
Water Content Comparison of Green Bean and Roasted Bean of Robusta Gumitir Coffee Based on Processing Method and Roast Level
Authors: Alwi Annisa Lutfi , Nuraisyah Anni , Ulma Zeni , Mastutik Lilik , Nirmala Kusumaningtyas Rizky
Published: June 2023

This study aims to compare the water content of green beans and roasted coffee bean based on the processing method and roasting level. The research was conducted at the TEFA Coffee Stub-Agricultural Plant Processing Laboratory, Politeknik Negeri Jember in April-July 2022. The coffee samples were Robusta coffee originating from the Gumitir area, Jember. This research consists of two main stages, green bean processing (dry (natural), honey, semi-wet, and full wash process) and roasting process based on light, medium, and dark levels. The analysis result showed that there was a decrease in the water content of coffee beans in each processing method as the roasting level increased. Overall, the water content of roast beans from full wash processing has the highest level compared to roast beans from other processed methods. The water content of green beans was originally 12.65%, decreased to 3.91%, 3.90%, and 3.38% after roasting with light, medium, and dark levels. The darker the roast level, the more weight loss. A significant decrease in water content was obtained in green beans processed by the honey method. Initially, the green bean water content was 13.05%, decreasing to 2.36%, 2.14, and 1.34% at each roasting level. Meanwhile, the green beans produced from natural processing (dry process), semi-wet, and full wash resulted in a decrease in water content that was not significant as the roasting level increased. Based on the results of this study, it is necessary to carry out further research related to the relationship between processing methods and roasting level on the physicochemical characteristics of coffee beans by optimizing.
Biochemical characterization of fermented Yerba Mate (Ilex paraguariensis) using Rhizopus oligosporus
Authors: Kwak So-Hyung , Kim Hayeong , Jeon Ji hyeon , Pal Kunal , Kang Dong-Hyun , Kim Doman
Published: June 2023

Solid-state fermentation (SSF) was used to enhance the bioactive compounds and biological properties of food materials, such as buckwheat, turmeric, and ginseng. This study was investigated the effects of SSF for up to 10 days using Rhizopus oligosporus on Yerba mate (Ilex paraguariensis St. Hilaire), which was done to enhance its biological properties. The total phenolic and saponin contents of Yerba mate rose to 19.92% and 38.04% after 1- and 7-day fermentation, respectively. Furthermore, chlorogenic acid, caffeic acid, and caffeine levels were increased up to 27.74% by fermentation, as determined by UPLC-MS analysis. ORAC and FRAP assays showed that the antioxidant activities of Yerba mate were enhanced 1.9- and 1.14-fold after 1-day fermentation. In addition, its inhibitory activities against yeast α-glucosidase and nitric oxide release in LPS-stimulated RAW264.7 cells were higher than in the unfermented Yerba mate. Moreover, taste sensory analysis using an electronic tongue sensory system showed that the flavor of Yerba mate after 1-day fermentation was similar to that of the unfermented Yerba mate. These results suggested that solid fermentation using R. oligosporus is conducive to producing Yerba mate with enhanced biological properties.
High prevalence of zero-dose children in underserved and special setting populations in Ethiopia using a generalize estimating equation and concentration index analysis
Authors: Biks Gashaw , Shiferie Fisseha , Tsegaye Dawit , Asefa Wondwossen , Alemayehu Legese , Wondie Tamiru , Zelalem Meseret , Lakew Yohannes , Belete Kidist , Gebremedhin Samson
Published: June 2023

Background Globally, according to the WHO/UNICEF 2021 estimates, more than 18 million children in low- and middle-income countries, primarily in Africa and South-East Asia, are not receiving any vaccinations. Ethiopia is one of the top four countries contributing to the global number of zero-dose children. Objective To estimate the prevalence of zero-dose children and associated factors in underserved populations of Ethiopia. Methods A cross-sectional vaccine coverage survey was conducted in June 2022. The study participants were mothers of children aged 12–35 months. Data were collected using the CommCare application system and later analysed using Stata version 17. Vaccination coverage was estimated using a weighted analysis approach. A generalized estimating equation model was fitted to determine the predictors of zero-dose children. An adjusted odds ratio (AOR) with 95% confidence interval (CI) and a p-value of 0.05 or less was considered statistically significant. Results The overall prevalence of zero-dose children in the study settings was 33.7% (95% CI: 34.9%, 75.7%). Developing and pastoralist regions, internally displaced peoples, newly formed regions, and conflict-affected areas had the highest prevalence of zero-dose children. Wealth index (poorest [AOR = 2.78; 95% CI: 1.70, 4.53], poorer [AOR = 1.96; 95% CI: 1.02, 3.77]), single marital status [AOR = 2.4; 95% CI: 1.7, 3.3], and maternal age (15–24 years) [AOR = 1.2; 95% CI: 1.1, 1.3] were identified as key determinant factors of zero-dose children in the study settings. Additional factors included fewer than four ANC visits [AOR = 1.3; 95% CI: 1.2, 1.4], not receiving PNC services [AOR = 2.1; 95% CI: 1.5, 3.0], unavailability of health facilities within the village [AOR = 3.7; 95% CI: 2.6, 5.4], women-headed household [AOR = 1.3; 95% CI:1.02, 1.7], low gender empowerment [AOR = 1.6; 95% CI: 1.3, 2.1], and medium gender empowerment [AOR = 1.7; 95% CI: 1.2, 2.5]. Conclusion In the study settings, the prevalence of zero-dose children is very high. Poor economic status, disempowerment of women, being unmarried, young maternal age, and underutilizing antenatal or post-natal services are the important predictors. Therefore, it is recommended to target tailored integrated and context-specific service delivery approach. Moreover, extend immunization sessions opening hours during the evening/weekend in the city administrations to meet parents’ needs.
Development of a novel colon adenocarcinoma m6A-related lncRNA pair prognostic model
Authors: Liang Shengmei , Qiu Xinze , Chen Da , Cai Lulu , Wei Fangyou , Huang Jiean , Liu Shiquan
Published: June 2023

Background: Colon adenocarcinoma (COAD) is among the most prevalent malignancies. N6-methyladenosine (m6A) alterations, the most prevalent RNA modification, can influence COAD progress. In addition, long noncoding RNA (lncRNA) plays an important role in COAD and is closely related to m6A modification. However, the prognostic value of lncRNAs associated to m6A in COAD is unknown.
Methods: In present study, the information from The Cancer Genome Atlas (TCGA) was employed to examine the predictive relevance of m6A-related lncRNA pair signatures in COAD. M6A-related lncRNAs was identified based on co-expression analysis utilizing the Pearson correlation. Then, the lncRNAs paired related to prognosis were identified, followed by univariate Cox regression analysis. The receiver operating characteristic (ROC) curves for predicting overall survival (OS) were conducted by using the least absolute shrinkage and selection operator (LASSO) penalized Cox analysis to identify and construct a risk score prognostic model. After determining if it was an independent prognostic factor, relationships between the risk score model and clinical traits, immune-related factors, and medication sensitivity analysis were analysed.
Results: A total of 319 m6A-related lncRNA pairs were found, and 35 of which were connected to a predictive pattern for risk scores. The risk score model was proven to be an independent predictive factor and was notably superior to the clinicopathological features. Correlation analyses revealed differences between high- and low-risk groups in clinicopathological characteristics, immune-related factors, and drug sensitivity analysis. Conclusions: The novel COAD prognostic model based on paired differentially expressed m6A-related lncRNAs showed promising clinical predictive value.
Conclusions: The novel COAD prognostic model based on paired differentially expressed m6A-related lncRNAs showed promising clinical predictive value.
The Role of L-histidine on Nickel Translocation and Antioxidant enzymes activity in Hyperaccumulator (Odontarrhena inflata) and Non-accumulator (Aurinia saxatilis) plants
Authors: Soleymanifar Soraya , Ehsanpour Ali Akbar , Ghasemi Rasoul , Schat Henk
Published: June 2023

Background and Aims
The role of L-histidine (L-His) in nickel (Ni) hyperaccumulation is not well known. The present study aimed to understand the impact of L-His on Ni translocation and Ni toxicity in shoots of Odontarrhena inflata and Aurinia saxatilis.
Methods
In the preliminary experiments, the Ni content of plants was quantified in pretreated plants using L-histidine and L-alanine (L-Ala) for 4 hours and then exposed to Ni for 8 hours. Hydrogen peroxide (H2O2) and activity of some antioxidant enzymes were determined after 4 hours of pretreatment using L-His and L-Ala before 48 hours Ni treatments.
Results
L-histidine increased Ni translocation to shoots in O. inflata and A. saxatilis. Ni increased the activity of POD, APX, and CAT in both species, but the higher activity of APX and CAT in O. inflata. Ni revealed a concentration-dependent increase in H2O2 content in A. saxatilis. L-His pretreatment increased H2O2 concentration in Ni-treated plants. Pretreatment with L-His decreased the activity of POD, APX, and CAT only at 300 µM Ni in O. inflata while decreasing the activity of CAT, but increased POD activity at 150 and 300 µM Ni in A. saxatilis. Pretreatment with L-Ala decreased POD and APX activity but had no significant impact on H2O2 content and CAT activity.
Conclusion
L-Histidine promoted root-to-shoot Ni translocation and alleviated Ni toxicity by inducing of antioxidant enzymes in hyperaccumulator and non-accumulator plants. The role of histidine in Ni hyperaccumulation may not be limited to Ni transport, but linked to detoxification of Ni.