bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementing care coordination in a large dental care organization in the United States by upskilling front office personnel
Authors: Kottek Aubri M. , Hoeft Kristin S. , White Joel M. , Simmons Kristen , Mertz Elizabeth A.
Published: April 2021

Background

Care coordination is a key strategy used to improve health outcomes and efficiency, yet there are limited examples in dentistry. A large dental accountable care organization piloted care coordination by retraining existing administrative staff to coordinate the care of high-risk patients. Following the pilot’s success, a formal “dental care advocate” (DCA) role was integrated system-wide. The goal of this new role is to improve care, patient engagement, and health outcomes while integrating staff into the clinical care team. We aim to describe the process of DCA role implementation and assess staff and clinician perceptions about the role pre- and post-implementation.

Methods

Guided by the Consolidated Framework for Implementation Research, semi-structured interviews with clinical and operational administrative staff and observation at the company-wide training session were combined with pre- and post-implementation electronic surveys. Descriptive statistics and mean scores were tested for significance between each survey sample (t-tests), and qualitative data were thematically analyzed.

Results

With preliminary evidence from the pilot and strong executive support, a dedicated leadership team executed a stepwise rollout of the DCA role over 6 months. Success was facilitated by an organizational culture of frequent interventions deployed rapidly through a centralized system, along with supportive buy-in from managerial teams and high staff acceptance and enthusiasm for the DCA role before implementation. Following implementation, significant changes in attitudes and beliefs about the role were measured, though managers held stronger positive impressions than DCAs. DCAs reported high confidence in new skills and dental knowledge post-implementation, including motivational interviewing and the ability to confidently answer patients’ questions about their oral health. Overall, the fast-paced implementation of this new role was well received, although consistent and significant differences in mean attitudes between managers and DCAs indicate more work to fine-tune the role is needed.

Conclusions

Successful implementation of the new DCA role was facilitated by a strong organizational commitment to team-based dentistry and positive impressions of care coordination among staff and managers. Upskilling existing administrative staff with the necessary training to manage some high-risk patient needs is one method that can be used to implement care coordination efforts in dentistry.
Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model
Authors: Wu Jianjun , Xie Shoupin , Li Hailong , Zhang Yanxia , Yue Jia , Yan Chunlu , Liu Kai , Liu Yongqi , Xu Rui , Zheng Guisen
Published: April 2021

Background

Glioma is a complex cancer with a high morbidity and high mortality. Bone marrow mesenchymal stem cells (BMSCs) have shown promise as an excellent cell/drug delivery vehicle for gene-targeted therapy; however, maintaining genetic stability and biological activity remains difficult. Furthermore, whether BMSCs support or inhibit tumor growth remains debated. This study investigated whether a traditional Chinese medicine fomular, Fuzheng Yiliu decoction (FYD) had a synergistic antitumor effect with IL-12 gene-modified BMSCs in glioma-bearing nude mice

Methods

The lentivirus-mediated IL-12 gene was transfected into primarily cultured BMSCs. A total of 72 BALB/c nude mice were used to establish xenograft models with glioma U251 cells and were divided into groups (n = 12) including blank control group, nude mouse model group (model group), lentiviral transfection of BMSC group with no gene loading (BMSC group), IL-12 lentivirus-transfected BMSC group (IL-12 + BMSC group), FYD treatment group (FYD group), and FYD treatment in IL-12 lentivirus-transfected BMSC group (FYD + IL-12 + BMSC group).. After treatment for 14 days, all mice were sacrificed to collect tumor tissue and serum for more detection, such as distribution of BMSCs, cell apoptosis in xenograft tumors, serum IL-12 and INF-γ levels, mouse weight and tumor volume were measured

Results

There were significantly more apoptotic cells in tumor tissue in IL-12 gene transfected group, FYD treatment group and FYD combining with IL-12 gene transfected group than that in the model group (P < 0.05). The FYD + IL-12 + BMSC group showed significantly higher Bax and lower Bcl-2 expression (P < 0.05), and serum IL-12 and INF-γ levels (P < 0.05) were higher than that in all other groups. After the intervention, this group also showed a strong inhibitory effect against tumor growth (P < 0.05)

Conclusions

This study suggested FYD treatment combined with IL-12 gene-modified BMSCs shows synergistic antitumor effect in glioma-bearing nude mice.
Development of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses
Authors: Li Ge , Wang Xun , Li Qingmei , Yang Jifei , Liu Xiao , Qi Wenbao , Guo Junqing , Deng Ruiguang , Zhang Gaiping
Published: April 2021

Background

H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection.

Methods

The H7N9 AIV was inactivated and purified, and was used as the antigen to immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced through the hybridoma technique. The McAb 10A8 was conjugated with colloid gold as detecting antibody; McAb 9B6 was dispensed on the nitrocellulose membran as the capture test line and the Goat-anti mouse IgG antibody was dispensed as control line respectively. The immunochromatographic strip was prepared.

Results

The analysis of ELISA and virus neutralization test showed that the obtained McAbs specifically recognized H7 HA. Based on the prepared strip, the detection of H7 AIV was achieved within 10 min. No cross-reaction occurred between H7 AIVs and other tested viruses. The detection limit of the strip for H7 was 2.4 log10EID50/0.1 mL for chicken swab samples.

Conclusion

The McAbs were specific for H7 and the immunochromatographic strip developed in this study was convenient, rapid and reliable for the detection of H7 AIV. The strip could provide an effective method for the rapid and early detection of H7 AIV.
An in vitro model for assessment of SARS-CoV-2 infectivity by defining the correlation between virus isolation and quantitative PCR value: isolation success of SARS-CoV-2 from oropharyngeal swabs correlates negatively with Cq value
Authors: Sonnleitner Sissy Therese , Dorighi Julian , Jansen Bianca , Schönegger Carmen , Gietl Sarah , Koblmüller Stephan , Sturmbauer Christian , Posch Wilfried , Walder Gernot
Published: April 2021

Background

At the beginning of the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), little was known about its actual rate of infectivity and any COVID-19 patient positive in laboratory testing was supposed to be highly infective and a public health risk factor.

Methods

One hundred oropharyngeal samples were obtained during routine work flow of testing symptomatic persons by quantitative polymerase chain reaction (qPCR) and were inoculated onto cell culture of VeroB4 cells to study the degree of infectivity of SARS-CoV-2 in vitro. Quantification by virus titration and an external standard using synthetic RNA gave the breaking point of infectivity in SARS-CoV-2 in vitro.

Results

A clear negative correlation (r = − 0.76; p < 0.05) could be asserted between the viral load in quantitative polymerase chain reaction (qPCR) and the probability of a successful isolation in serial isolation experiments of specific oropharyngeal samples positive in qPCR. Quantification by virus titration and an external standard using synthetic RNA indicate a Cq between 27 and 30 in E-gene screening PCR as a breaking point in vitro, where infectivity decreases significantly and isolations become less probable.

Conclusions

This study showed that only the 21% of samples with the highest viral load were infectious enough to transmit the virus in vitro and determined that the dispersion rate in vitro is surprisingly close to those calculated in large retrospective epidemiological studies for SARS-CoV-2. This raises the question of whether this simple in vitro model is suitable to give first insights in dispersion characters of novel or neglected viral pathogens. The statement that SARS-CoV-2 needs at least 40,000 copies to reliably induce infection in vitro is an indication of its transmissibility in Public Health decisions. Applying quantitative PCR systems in diagnosis of SARS-CoV2 can distinguish between patients providing a high risk of transmission and those, where the risk of transmission is probably limited to close and long-lasting contacts.
Clinical and genetic characteristics of hypophosphatasia in Chinese children
Authors: Liu Meijuan , Liu Min , Liang Xuejun , Wu Di , Li Wenjing , Su Chang , Cao Bingyan , Chen Jiajia , Gong Chunxiu
Published: April 2021

Background

Hypophosphatasia (HPP) is a rare inherited disorder, which is caused by loss-of-function mutations in the ALPL gene. HPP is a heterogeneous disease that has a wide spectrum of phenotypes. Few studies were carried out in the Chinese population with HPP, especially in children.

Methods

The clinical and genetic characteristics of 10 Chinese children with HPP who were referred to the Beijing Children’s Hospital were described. Previously reported HPP cases of children in China were also reviewed.

Results

A total of 33 cases were identified, which included 2 perinatal lethal HPP, 10 infantile HPP, 10 childhood HPP, and 11 odonto HPP. The male-to-female ratio was 24:9. The average age at onset was 0.69 years (ranged from 2 h after birth to 14 years), while the average age at clinical diagnosis was 3.87 years (ranged from 2 h after birth to 19 years). Serum alkaline phosphatase (ALP) levels were significantly decreased in patients with perinatal lethal/infantile HPP when compared with those with the mild forms of HPP childhood/odonto HPP (P < 0.01). Although serum phosphate levels were not different (P > 0.05), serum calcium levels were elevated, and serum intact parathyroid hormone levels were decreased in patients with perinatal lethal/infantile HPP in comparison with those with the childhood/odonto HPP (P all < 0.01). Genetic analyses identified 40 mutations in 31 HPP cases, including 28 missense mutations, 9 frameshift mutations, 2 splice junction alterations, and 1 regulatory mutation. Of which, 5 novel mutations were identified in our present study: 2 frameshift mutations (p.Arg138GlyfsTer27, p.Leu511Profs*272); 2 missense mutations (p.Ala176Val, p.Phe268Leu), and 1 splice junction alteration (c.297+5G>A). Compound heterozygous mutations accounted for 80.6% of all variants. No mutational “hot-spot” was found. Most mutations of ALPL were located in exons 5, 7, 10, and 3. Notably, subjects that carrying single heterozygous mutations showed milder phenotypes of HPP, while subjects with nonsense mutations were associated with a severer phenotype.

Conclusions

HPP is a rare disease with often delayed diagnosis, and the incidence of HPP in China may be seriously underestimated. The present study expands the phenotypic and genotypic spectrum and the understanding of HPP in Chinese children. These findings will be useful for clinical assessment and shorten the diagnosis time for pediatric HPP in China.
Lactoferrin is required for early B cell development in C57BL/6 mice
Authors: Wei Lingyu , Liu Can , Wang Jia , Zheng Xiang , Peng Qiu , Ye Qiurong , Qin Zailong , Li Zhengshuo , Zhang Xiaoyue , Wu Yangge , Wen Yuqing , Zhang Xuemei , Yan Qun , Ma Jian
Published: April 2021

Lactoferrin (Lf) is widely distributed in mammalian milk, various tissues, and their exocrine fluids and has many physiological functions, such as bacteriostasis, antivirus, and immunoregulation. Here, we provide evidence that lactoferrin is required for early stages of B cell development in mice. Lactoferrin-deficient (Lf−/−) C57BL/6 mice showed systematic reduction in total B cells, which was attributed to the arrest of early B cell development from pre-pro-B to pro-B stage. Although the Lf−/− B cell “seeds” generated greater pro-B cells comparing to wild type (WT) littermates, the Lf−/− mice bone marrow had less stromal cells, and lower CXCL12 expression, produced a less favorable “microenvironment” for early B cell development. The underlying mechanism was mediated through ERK and AKT signalings and an abnormality in the transcription factors related to early differentiation of B cells. The Lf−/− mice also displayed abnormal antibody production in T cell-dependent and T cell-independent immunization experiments. In a pristane-induced lupus model, Lf−/− mice had more serious symptoms than WT mice, whereas lactoferrin treatment alleviated these symptoms. This study demonstrates a novel role of lactoferrin in early B cell development, suggesting a potential benefit for using lactoferrin in B cell-related diseases.
Treating the symptom or treating the disease in neonatal seizures: a systematic review of the literature
Authors: Falsaperla Raffaele , Scalia Bruna , Giugno Andrea , Pavone Piero , Motta Milena , Caccamo Martina , Ruggieri Martino
Published: April 2021

Aim

The existing treatment options for neonatal seizures have expanded over the last few decades, but no consensus has been reached regarding the optimal therapeutic protocols. We systematically reviewed the available literature examining neonatal seizure treatments to clarify which drugs are the most effective for the treatment of specific neurologic disorders in newborns.

Method

We reviewed all available, published, literature, identified using PubMed (published between August 1949 and November 2020), that focused on the pharmacological treatment of electroencephalogram (EEG)-confirmed neonatal seizures.

Results

Our search identified 427 articles, of which 67 were included in this review. Current knowledge allowed us to highlight the good clinical and electrographic responses of genetic early-onset epilepsies to sodium channel blockers and the overall good response to levetiracetam, whose administration has also been demonstrated to be safe in both full-term and preterm newborns.

Interpretation

Our work contributes by confirming the limited availability of evidence that can be used to guide the use of anticonvulsants to treat newborns in clinical practice and examining the efficacy and potentially harmful side effects of currently available drugs when used to treat the developing newborn brain; therefore, our work might also serve as a clinical reference for future studies.
Bioethanolic yeasts from dung beetles: tapping the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation
Authors: Nwaefuna Anita Ejiro , Rumbold Karl , Boekhout Teun , Zhou Nerve
Published: April 2021

Bioethanol from abundant and inexpensive agricultural and industrial wastes possesses the potential to reduce greenhouse gas emissions. Bioethanol as renewable fuel addresses elevated production costs, as well as food security concerns. Although technical advancements in simultaneous saccharification and fermentation have reduced the cost of production, one major drawback of this technology is that the pre-treatment process creates environmental stressors inhibitory to fermentative yeasts subsequently reducing bioethanol productivity. Robust fermentative yeasts with extreme stress tolerance remain limited. This review presents the potential of dung beetles from pristine and unexplored environments as an attractive source of extremophilic bioethanolic yeasts. Dung beetles survive on a recalcitrant lignocellulose-rich diet suggesting the presence of symbiotic yeasts with a cellulolytic potential. Dung beetles inhabiting extreme stress environments have the potential to harbour yeasts with the ability to withstand inhibitory environmental stresses typically associated with bioethanol production. The review further discusses established methods used to isolate bioethanolic yeasts, from dung beetles.
Effect of integrated treatment on enhancing the enzymatic hydrolysis of cocksfoot grass and the structural characteristics of co-produced hemicelluloses
Authors: Sun Shao-Chao , Sun Dan , Cao Xue-Fei
Published: April 2021

Background

Cocksfoot grass (Dactylis glomerataL.) with high biomass yield and rich cellulose can be used to produce bioethanol as fuel additive. In view of this, ultrasonic and hydrothermal pretreatments followed by successive alkali extractions were assembled into an integrated biorefinery process applied on cocksfoot grass to improve its enzymatic hydrolysis. In this work, the effects of ultrasonic and hydrothermal pretreatments followed by sequential alkali extractions on the enzymatic hydrolysis of cocksfoot grass were investigated. In addition, since large amount of hemicelluloses were released during the hydrothermal pretreatment and alkali extraction process, the yields, structural characteristics and differentials of water- and alkali-soluble hemicellulosic fractions isolated from different treatments were also comparatively explored.

Results

The integrated treatment significantly removed amorphous hemicelluloses and lignin, resulting in increased crystallinity of the treated residues. A maximum saccharification rate of 95.1% was obtained from the cellulose-rich substrate after the integrated treatment. In addition, the considerable hemicelluloses (31.4% water-soluble hemicelluloses and 53.4% alkali-soluble hemicelluloses) were isolated during the integrated treatment. The released water-soluble hemicellulosic fractions were found to be more branched as compared with the alkali-soluble hemicellulosic fractions and all hemicellulosic fractions were mixed polysaccharides mainly composed of branched xylans andβ-glucans.

Conclusion

The combination of ultrasonic and hydrothermal pretreatments followed by successive alkali extractions can dramatically increase the enzymatic saccharification rate of the substrates and produce considerable amounts of hemicelluloses. Detailed information about the enzymatic hydrolysis rates of the treated substrates and the structural characteristics of the co-produced hemicelluloses will help the synergistic utilization of cellulose and hemicellulose in cocksfoot grass.
An evaluation of repellency and feeding inhibition of ethno-medicinal plants against major malaria vectors in southern Ethiopia
Authors: Olbamo Tekle , Esayas Endashaw , Gebre Tizazu , Massebo Fekadu
Published: April 2021

Background

Plant-based mosquito control methods may use as a supplementary malaria vector control strategy. This study aimed to evaluate the effect of smoking ethno-medicinal plants on indoor density and feeding activity of malaria vectors at early hours of the night and its residual effect after midnight in southern Ethiopia.

Methods

Both field and tent trials were conducted to evaluate the impact of smoking Juniperus procera leaves, Eucalyptus globulus seeds and Olea europaea leaves in Kolla Shara Village from July 2016 to February 2017. For the field trial, five grass-thatched traditional huts (three for ethno-medicinal plants and two as control [only charcoal smoking and non-charcoal smoking]) were used. Indoor host-seeking mosquitoes were collected by CDC light traps. A Latin square design was employed to minimize the bias due to the variation in house location and different sampling nights. For the tent experiment, 25 3–5-day-old starved wild female Anopheles mosquitoes reared from the larvae were released into the tents where a calf was tethered at the mid-point of each tent.

Results

A total of 614 Anopheles mosquitoes belonging to 5 species were collected from 5 huts, of which 93.4% was An. arabiensis; O. europaea, E. globulus and J. procera reduced the indoor density of An. arabiensis, with the mean percentage drop of 80%, 73% and 70%, respectively. In the tent trial, smoking of these plants had significant knockdown effects and inhibited feeding on the calves (F = 383.5, DF = 3, P < 0.01). The mean knockdown effect due to O. europaea was relatively high (17.7 ± 0.54; 95% CI 16.8–18.6), while it was only 0.9 ± 0.1 (95% CI 0.29–1.52) in the control tents. All the test plants used in the tent trial caused significantly inhibited feeding activity of An. arabiensis on the host (F = 383.5, DF = 3, P < 0.01). About 94.5%, 89.5% and 86% of mosquitoes were unfed because of the smoking effect of O. europaea, E. globulus and J. procera, respectively, whereas only 19.5% were unfed in the control tent.

Conclusions

Smoking ethno-medicinal plant materials reduced indoor density of malaria vectors and inhibited feeding on calves inside the tents. Thus, plant-based mosquito control methods may play a vital role in reducing mosquito bites in the early hours of the night and thereby reduce residual malaria transmission.

Graphical Abstract
Detection of malaria parasites in samples from returning US travelers using the Alethia® Malaria Plus LAMP assay
Authors: Ljolje Dragan , Abdallah Rispah , Lucchi Naomi W.
Published: April 2021

Objective

In this study, the performance of a commercially available malaria LAMP assay (Alethia® Malaria Plus LAMP) was evaluated using retrospective clinical samples obtained from travelers returning to the United States of America (USA). Recently, several laboratories in non-malaria endemic countries evaluated the use of the loop mediated isothermal amplification (LAMP) assays for the diagnosis of imported malaria cases. These tests are simpler than polymerase-chain reaction (PCR)-based assays and were shown to have high sensitivity. Much of malaria diagnoses in the USA, is undertaken at the state level using mainly microscopy and rapid diagnostic tests (RDTs). However, molecular tools offer greater sensitivity over microscopy and RDTs. A reliable, easy to perform molecular assay can provide a test of choice for the accurate detection of malaria parasites in places where expert microscopy is lacking and/or for the detection of low-parasite density infections.

Results

The Alethia® Malaria Plus LAMP assay was easy to use, had similar test performances as the real-time PCR reference test and results were obtained faster (within 1 h) than the reference test. The sensitivity of the assay was 100% with a kappa score of 1 when compared to the reference PET-PCR assay.
The new accounting for expected adjusted effect test (AEAE test) has higher positive predictive value than a zero-order significance test
Authors: Sorjonen Kimmo , Nilsonne Gustav , Melin Bo , Ingre Michael
Published: April 2021

Objective

The present simulation study aimed to assess positive predictive value (PPV) and negative predictive value (NPV) for our newly introduced Accounting for Expected Adjusted Effect test (AEAE test) and compare it to PPV and NPV for a traditional zero-order significance test.

Results

The AEAE test exhibited greater PPV compared to a traditional zero-order significance test, especially with a strong true adjusted effect, low prior probability, high degree of confounding, large sample size, high reliability in the measurement of predictor X and outcome Y, and low reliability in the measurement of confounder Z. The zero-order significance test, on the other hand, exhibited higher NPV, except for some combinations of high degree of confounding and large sample size, or low reliability in the measurement of Z and high reliability in the measurement of X/Y, in which case the zero-order significance test can be completely uninformative. Taken together, the findings demonstrate desirable statistical properties for the AEAE test compared to a traditional zero-order significance test.
Actor-critic learning-based energy optimization for UAV access and backhaul networks
Authors: Yuan Yaxiong , Lei Lei , Vu Thang X. , Chatzinotas Symeon , Sun Sumei , Ottersten Björn
Published: April 2021

In unmanned aerial vehicle (UAV)-assisted networks, UAV acts as an aerial base station which acquires the requested data via backhaul link and then serves ground users (GUs) through an access network. In this paper, we investigate an energy minimization problem with a limited power supply for both backhaul and access links. The difficulties for solving such a non-convex and combinatorial problem lie at the high computational complexity/time. In solution development, we consider the approaches from both actor-critic deep reinforcement learning (AC-DRL) and optimization perspectives. First, two offline non-learning algorithms, i.e., an optimal and a heuristic algorithms, based on piecewise linear approximation and relaxation are developed as benchmarks. Second, toward real-time decision-making, we improve the conventional AC-DRL and propose two learning schemes: AC-based user group scheduling and backhaul power allocation (ACGP), and joint AC-based user group scheduling and optimization-based backhaul power allocation (ACGOP). Numerical results show that the computation time of both ACGP and ACGOP is reduced tenfold to hundredfold compared to the offline approaches, and ACGOP is better than ACGP in energy savings. The results also verify the superiority of proposed learning solutions in terms of guaranteeing the feasibility and minimizing the system energy compared to the conventional AC-DRL.
An experimental publish-subscribe monitoring assessment to Beyond 5G networks
Authors: Perez Ramon , Garcia-Reinoso Jaime , Zabala Aitor , Serrano Pablo , Banchs Albert
Published: April 2021

The fifth generation (5G) of mobile networks is designed to accommodate different types of use cases, each of them with different and stringent requirements and key performance indicators (KPIs). To support the optimization of the network performance and validation of the KPIs, there exist the necessity of a flexible and efficient monitoring system and capable of realizing multi-site and multi-stakeholder scenarios. Nevertheless, for the evolution from 5G to 6G, the network is envisioned as a user-driven, distributed Cloud computing system where the resource pool is foreseen to integrate the participating users. In this paper, we present a distributed monitoring architecture for Beyond 5G multi-site platforms, where different stakeholders share the resource pool in a distributed environment. Taking advantage of the usage of publish-subscribe mechanisms adapted to the Edge, the developed lightweight monitoring solution can manage large amounts of real-time traffic generated by the applications located in the resource pool. We assess the performance of the implemented paradigm, revealing some interesting insights about the platform, such as the effect caused by the throughput of monitoring data in performance parameters such as the latency and packet loss, or the presence of a saturation effect due to software limitations that impacts in the performance of the system under specific conditions. In the end, the performance evaluation process has confirmed that the monitoring platform suits the requirements of the proposed scenarios, being capable of handling similar workloads in real 5G and Beyond 5G scenarios, then discussing how the architecture could be mapped to these real scenarios.
A study about signal variation with minor receiver displacement in a meeting room at 60 GHz: measurements and simulations
Authors: Sheikh Muhammad Usman , Ruttik Kalle , Jäntti Riku , Hämäläinen Jyri
Published: April 2021

The aim of this work is to study the impact of small receiver displacement on a signal propagation in a typical conference room environment at a millimeter wave frequency of 60 GHz. While channel measurements provide insights on the propagation phenomena, their use for the wireless system performance evaluation is challenging. Whereas, carefully executed three-dimensional ray tracing (RT) simulations represent a more flexible option. Nevertheless, a careful validation of simulation methodology is needed. The first target of this article is to highlight the benefits of an in-house built three-dimensional RT tool at 60 GHz and shows the effectiveness of simulations in predicting different characteristics of the channel. To validate the simulation results against the measurements, two different transmitter (Tx) positions and antenna types along with ten receiver (Rx) positions are considered in a typical conference room. In first system configuration, an omnidirectional antenna is placed in the middle of the table, while in the second system configuration a directed horn antenna is located in the corner of the meeting room. After validating the simulation results with the measurement data, in the second part of this work, the impact of a small change, i.e., 20 cm in the receiver position, is studied. To characterize the impact, we apply as performance indicators the received power level, root mean square delay spread (RMS-DS) and RMS angular spread (RMS-AS) in azimuth plane. The channel characteristics are considered with respect to the direct orientation (DO), i.e., the Rx antenna is directed toward the strongest incoming path. Different antenna configurations at the Tx and Rx side are applied to highlight the role of antenna properties on the considered channel characteristics. Especially, in the second system configuration the impact of different antenna half power beamwidth on different considered channel characteristics is highlighted through acquired simulation results. The validation of results shows the RMS error of only 2–3 dB between the measured and simulated received power levels for different Tx configurations in the direction of DO. Results indicate that only a small change of the Rx position may result a large difference in the received power level even in the presence of line-of-sight between the Tx and Rx. It is found that the STD of received power level across the room increases with the decrease in HPBW of the antenna. As can be expected, directed antennas offer lower value of RMS-DS and RMS-AS compared with isotropic antenna.
Efficient scalar multiplication of ECC using SMBR and fast septuple formula for IoT
Authors: Guo Chong , Gong Bei
Published: April 2021

In order to solve the problem between low power of Internet of Things devices and the high cost of cryptography, lightweight cryptography is required. The improvement of the scalar multiplication can effectively reduce the complexity of elliptic curve cryptography (ECC). In this paper, we propose a fast formula for point septupling on elliptic curves over binary fields using division polynomial and multiplexing of intermediate values to accelerate the computation by more than 14%. We also propose a scalar multiplication algorithm based on the step multi-base representation using point halving and the septuple formula we proposed, which significantly reduces the computational cost. The experimental results show that our method is more efficient over binary fields and contributes to reducing the complexity of ECC.
Practical cross-layer testing of HARQ-induced delay variation on IP/RTP QoS and VoLTE QoE
Authors: Lipovac Adriana , Lipovac Vlatko , Grbavac Ivan , Obradović Ines
Published: April 2021

As the PHY/MAC-layer IR-HARQ and RLC-layer ARQ error recovery procedures, adopted in LTE, may impose additional delay when their code-block retransmissions occur, the arising question is whether these significantly contribute to IP and consequently RTP packet delays, and finally degrade the overall application-layer end-to-end QoE, especially when voice is transmitted over LTE? With this regard, we propose and demonstrate a VoLTE QoS and QoE test procedure based on PHY/MAC/RLC/IP/TCP-UDP/RTP cross-layer protocol analysis and perceptual speech quality QoE measurements. We identified monotonic relationship between the paired observations: QoE and HARQ RTT, i.e. between the PESQ voice quality rating and the IP/RTP packet latency, for given BLER of the received MAC/RLC code-blocks. Specifically, we found out that, for the HARQ RTT value of about 8 ms, only up to 2 HARQ retransmissions (and consequently no RLC-ARQ one) is appropriate during any voice packet, otherwise delay accumulation might not be accordingly “smoothed out” by jitter/playback buffers along the propagation path.
WNV-Detector: automated and scalable detection of wireless network vulnerabilities
Authors: Huang Yanxi , Zhu Fangzhou , Liu Liang , Meng Wezhi , Hu Simin , Ye Renjun , Lv Ting
Published: April 2021

The security of wireless routers receives much attention given by the increasing security threats. In the era of Internet of Things, many devices pose security vulnerabilities, and there is a significant need to analyze the current security status of devices. In this paper, we develop WNV-Detector, a universal and scalable framework for detecting wireless network vulnerabilities. Based on semantic analysis and named entities recognition, we design rules for automatic device identification of wireless access points and routers. The rules are automatically generated based on the information extracted from the admin webpages, and can be updated with a semi-automated method. To detect the security status of devices, WNV-Detector aims to extract the critical identity information and retrieve known vulnerabilities. In the evaluation, we collect information through web crawlers and build a comprehensive vulnerability database. We also build a prototype system based on WNV-Detector and evaluate it with routers from various vendors on the market. Our results indicate that the effectiveness of our WNV-Detector, i.e., the success rate of vulnerability detection could achieve 95.5%.
Existence of solutions for fourth-order nonlinear boundary value problems
Authors: Huang Mingzhu
Published: April 2021

In this paper, we discuss the existence and approximation of solutions for a fourth-order nonlinear boundary value problem by using a quasilinearization technique. In the presence of a lower solutionαand an upper solutionβin the reverse order$\alpha \geq \beta $α≥β, we show the existence of (extreme) solution.
Delayed breast feeding initiation increases the odds of colostrum avoidance among mothers in Northwest Ethiopia: a community-based cross-sectional study
Authors: G/slassie Maezu , Azene Zelalem Nigussie , Mulunesh Abuhay , Alamneh Tesfa Sewunet
Published: April 2021

Background

Colostrum is a yellowish and sticky breast milk produced in late pregnancy. Annually, 60% of 10.9 million under-five deaths globally are due to malnutrition. Of these, over two-thirds of the deaths are accounted by sub-optimal feeding practices in the first year of life, including colostrum discarding. However, evidence on the magnitude of colostrum avoidance and its associated factors at the community level is very limited in Ethiopia, particularly in the study area.

Thus, this study aimed to assess the magnitude of colostrum avoidance and associated factors among mothers who gave birth in the last six months in Gozamen district, northwest Ethiopia, 2019.

Methods

A community-based cross-sectional study was conducted among 741 (741) mothers who gave birth in the last six months in Gozamen district from August 1 to September 12, 2019. A stratified cluster sampling technique was used to select the study participants. Data were collected by face-to-face interviewer-administered, pretested, and semi-structured questionnaire. Binary logistic regressions (bi-variable and multivariable) were fitted to identify statistically significant variables. Adjusted Odds Ratio (AOR) with 95% Confidence Interval (CI) was used to declare statistically significant variables on the basis of p-value < 0.05 in the multivariable binary logistic regression.

Results

This study indicated that the magnitude of colostrum avoidance was 22.1% (95% CI, 19.0, 25.2%).

Mothers who did not get counseling on timely initiation of breast feeding (AOR = 3.91[95% CI, 1.98, 7.72]), not participate in pregnant woman forum (AOR = 2.59[95% CI, 1.30, 5.14]), initiate breast-feeding lately (more than 1 h) (AOR 2.27[95% CI, 1.18, 4.34]), and those having unfavorable attitude towards colostrum feeding (AOR = 7.35[95% CI, 3.89, 13.91]) were factors associated with the increased likelihood of colostrum avoidance. However, institutional delivery (AOR; 0.06[95% CI, 0.02, 0.19]) and prelacteal feeding (AOR; 0.10[95% CI, 0.05, 0.21]) were predictors associated with reduced likelihood of colostrum avoidance.

Conclusion

Colostrum avoidance is a common practice in the study area. Therefore, in order to reduce this practice, strengthening infant feeding counseling, promoting institutional delivery, timely initiation of breastfeeding, health education, and community advocating are recommended interventions. In addition, creating awareness on the benefits of colostrum feeding is very instrumental to tackle the practice of colostrum avoidance.
Use of biomass-derived adsorbents for the removal of petroleum pollutants from water: a mini-review
Authors: Vahabisani Azar , An Chunjiang
Published: April 2021

Over the past decades, a large amount of petroleum pollutants has been released into the environment resulting from various activities related to petrochemicals. The discharge of wastewater with petrochemicals can pose considerable risk of harm to the human health and the environment. The use of adsorbents has received much consideration across the environmental field as an effective approach for organic pollutant removal. There is a particular interest in the use of biomass adsorbent as a promising environmentally-friendly and low-cost option for removing pollutants. In this article, we present a review of biomass-derived adsorbents for the removal of petroleum pollutants from water. The features of different adsorbents such as algae, fungi, and bacteria biomasses are summarized, as is the process of removing oil and PAHs using biomass-derived adsorbents. Finally, recommendations for future study are proposed.
Phenolic compounds as natural feed additives in poultry and swine diets: a review
Authors: Mahfuz Shad , Shang Qinghui , Piao Xiangshu
Published: April 2021

Due to ban on using antibiotics in feed industry, awareness of using natural feed additives have led to a great demand. The interest of plants phenolic compounds as a potential natural antioxidant source has been considered in research community due to their predictable potential role as feed additives in poultry and swine production. However, the mode of action for their functional role and dosage recommendation in animal diets are still remain indistinct. Taking into account, the present review study highlights an outline about the mode of action of phenolic compound and their experimental uses in poultry and swine focusing on the growth performance, antioxidant function, immune function, antimicrobial role and overall health status, justified with the past findings till to date. Finally, the present review study concluded that supplementation of phenolic compounds as natural feed additives may have a role on the antioxidant, immunity, antimicrobial and overall production performance in poultry and swine.
Coenzyme Q10 protected against arsenite and enhanced the capacity of 2,3-dimercaptosuccinic acid to ameliorate arsenite-induced toxicity in mice
Authors: Mwaeni Victoria K. , Nyariki James N. , Jillani Ngalla , Omwenga George , Ngugi Mathew , Isaac Alfred Orina
Published: April 2021

Background

Arsenic poisoning affects millions of people. The inorganic forms of arsenic are more toxic. Treatment for arsenic poisoning relies on chelation of extracellularly circulating arsenic molecules by 2,3-dimecaptosuccinic acid (DMSA). As a pharmacological intervention, DMSA is unable to chelate arsenic molecules from intracellular spaces. The consequence is continued toxicity and cell damage in the presence of DMSA. A two-pronged approach that removes extracellular arsenic, while protecting from the intracellular arsenic would provide a better pharmacotherapeutic outcome. In this study, Coenzyme Q10(CoQ10), which has been shown to protect from intracellular organic arsenic, was administered separately or with DMSA; following oral exposure to sodium meta-arsenite (NaAsO2) – a very toxic trivalent form of inorganic arsenic. The aim was to determine if CoQ10alone or when co-administered with DMSA would nullify arsenite-induced toxicity in mice.

Methods

Group one represented the control; the second group was treated with NaAsO2(15 mg/kg) daily for 30 days, the third, fourth and fifth groups of mice were given NaAsO2and treated with 200 mg/kg CoQ10(30 days) and 50 mg/kg DMSA (5 days) either alone or in combination.

Results

Administration of CoQ10and DMSA resulted in protection from arsenic-induced suppression of RBCs, haematocrit and hemoglobin levels. CoQ10and DMSA protected from arsenic-induced alteration of WBCs, basophils, neutrophils, monocytes, eosinophils and platelets. Arsenite-induced dyslipidemia was nullified by administration of CoQ10alone or in combination with DMSA. Arsenite induced a drastic depletion of the liver and brain GSH; that was significantly blocked by CoQ10and DMSA alone or in combination. Exposure to arsenite resulted in significant elevation of liver and kidney damage markers. The histological analysis of respective organs confirmed arsenic-induced organ damage, which was ameliorated by CoQ10alone or when co-administered with DMSA. When administered alone, DMSA did not prevent arsenic-driven tissue damage.

Conclusions

Findings from this study demonstrate that CoQ10and DMSA separately or in a combination, significantly protect against arsenic-driven toxicity in mice. It is evident that with further pre-clinical and clinical studies, an adjunct therapy that incorporates CoQ10alongside DMSA may find applications in nullifying arsenic-driven toxicity.
The efficacy of calcitriol treatment in non-alcoholic fatty liver patients with different genotypes of vitamin D receptor FokI polymorphism
Authors: Yaghooti Hamid , Ghanavati Fatemeh , Seyedian Seyed Saeed , Cheraghian Bahman , Mohammadtaghvaei Narges
Published: April 2021

Background

Vitamin D deficiency is prevalent in patients with non-alcoholic fatty liver disease (NAFLD), but there are debates on the usefulness of vitamin D treatment. The interindividual variations in response may be due to different genetic backgrounds.

The present study evaluated the efficacy of calcitriol treatment in NAFLD patients with regard to the vitamin D receptor (VDR) genotypes of FokI polymorphism.

Methods

The study was conducted on 128 NAFLD patients randomly divided into two groups and were subjected to intervention with 0.25 mcg calcitriol/day or placebo for 4 months, while anthropometric parameters, glycemic status, lipid profiles, inflammatory markers, liver enzymes, and fatty liver indices were measured. The ARMS-PCR method was used to genotype the VDR FokI polymorphism.

Results

Calcitriol treatments along with weight loss and diet recommendations decreased the liver enzymes (AST, ALT, and ALP, p < 0.001 for all) and fatty liver indices (HSI, p < 0.01 and APRI, p < 0.001), compared to the baseline. But when the calcitriol effects were compared to the placebo group, only ALP decrease remained significant (17.5 IU. P = 0.02). The prevalent FokI variants in our population were FF (53.1%) and Ff genotype (45.3%). No significant interaction of FokI variants to the calcitriol effects was found except for ALP. The decrease in the ALP activity was higher in calcitriol-received patients with the Ff genotype (p = 0.05).

Conclusions

The FF and Ff variants of VDR FokI polymorphism did not interact with the effects of calcitriol on fatty liver, but the ALP was more responsive in subjects with the Ff variant.

IRCT registration number

IRCT2017053034222N1 Registration date: 2017-06-28 - Retrospectively registered, https://en.irct.ir/trial/26203
Molecular classification of a complex structural rearrangement of the RB1 locus in an infant with sporadic, isolated, intracranial, sellar region retinoblastoma
Authors: Schieffer Kathleen M. , Feldman Alexander Z. , Kautto Esko A. , McGrath Sean , Miller Anthony R. , Hernandez-Gonzalez Maria Elena , LaHaye Stephanie , Miller Katherine E. , Koboldt Daniel C. , Brennan Patrick , Kelly Benjamin , Wetzel Amy , Agarwal Vibhuti , Shatara Margaret , Conley Suzanne , Rodriguez Diana P. , Abu-Arja Rolla , Shaikhkhalil Ala , Snuderl Matija , Orr Brent A. , Finlay Jonathan L. , Osorio Diana S. , Drapeau Annie I. , Leonard Jeffrey R. , Pierson Christopher R. , White Peter , Magrini Vincent , Mardis Elaine R. , Wilson Richard K. , Cottrell Catherine E. , Boué Daniel R.
Published: April 2021

Retinoblastoma is a childhood cancer of the retina involving germline or somatic alterations of the RB Transcriptional Corepressor 1 gene, RB1. Rare cases of sellar-suprasellar region retinoblastoma without evidence of ocular or pineal tumors have been described. A nine-month-old male presented with a sellar-suprasellar region mass. Histopathology showed an embryonal tumor with focal Flexner-Wintersteiner-like rosettes and loss of retinoblastoma protein (RB1) expression by immunohistochemistry. DNA array-based methylation profiling confidently classified the tumor as pineoblastoma group A/intracranial retinoblastoma. The patient was subsequently enrolled on an institutional translational cancer research protocol and underwent comprehensive molecular profiling, including paired tumor/normal exome and genome sequencing and RNA-sequencing of the tumor. Additionally, Pacific Biosciences (PacBio) Single Molecule Real Time (SMRT) sequencing was performed from comparator normal and disease-involved tissue to resolve complex structural variations. RNA-sequencing revealed multiple fusions clustered within 13q14.1-q21.3, including a novel in-frame fusion of RB1-SIAH3 predicted to prematurely truncate the RB1 protein. SMRT sequencing revealed a complex structural rearrangement spanning 13q14.11-q31.3, including two somatic structural variants within intron 17 of RB1. These events corresponded to the RB1-SIAH3 fusion and a novel RB1 rearrangement expected to correlate with the complete absence of RB1 protein expression. Comprehensive molecular analysis, including DNA array-based methylation profiling and sequencing-based methodologies, were critical for classification and understanding the complex mechanism of RB1 inactivation in this diagnostically challenging tumor.