bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Physical Activity and Physical Education With Overweight and Obesity Among School‐Aged Children and Adolescents in Pakistan: An Empirical Cross‐Sectional Study
Authors: Tanveer Moazzam , Asghar Ejaz , Badicu Georgian , Roy Nadeem , Siener Maximilian , Tanveer Umar , Al-Mhanna Sameer Badri , Batrakoulis Alexios
Published: October 2024

Background: Overweight and obesity in children and adolescents are pressing global public health concerns, including in Pakistan, where prevalence rates are rising. The long‐term health implications of these conditions underscore the need for targeted interventions during childhood and adolescence and periods when health behaviors are strongly influenced by external factors such as school, neighborhood, peers, and family.

Purpose: This study aimed to evaluate the relationship between physical activity (PA), physical education (PE), and overweight/obesity in school‐aged children and adolescents (ages 9–17) in Pakistan and to investigate their impact on obesity within this population.

Methods: A population‐based cross‐sectional study was conducted with a multistage random cluster sample of 4108 school‐aged children and adolescents from seven districts in Punjab, Pakistan. Overweight and obesity were assessed using World Health Organization (WHO) guidelines. Statistical analyses, including χ2 tests, Pearson correlation, linear regression, and logistic regression, were performed to examine the predictive characteristics and the impact of covariates on dichotomous outcomes. Statistical significance was set at p < 0.05, with 95% confidence intervals (CIs).

Results: The study included 4108 students (mean age = 13.92 years, 59.3% boys). The prevalence of obesity was 10.7% and overweight was 19.4%. A significant portion of students—83.8% of girls and 70.0% of boys—did not engage in at least 60 min of moderate to vigorous physical activity (MVPA) on more than 2 days per week. Urban students (79.2%) and those in private schools (79.3%) were particularly inactive. PA two times a week or less was strongly associated with higher obesity risk (OR: 3.47, 95% CI: 2.28–5.30, p < 0.001).

Conclusion: This study underscores the critical need to enhance PA opportunities in schools and communities in Pakistan, particularly for girls, to address the rising rates of overweight and obesity.
Supraglottic Neuroendocrine Carcinoma: A Case Report and Literature Review
Authors: Alhabib Renda , Zaidi Chaker , Alfryyan Abdulrahman , Albenayyan Hanan , Alotain Ibrahim
Published: October 2024

Neuroendocrine neoplasms (NENs) are rare in the head and neck region, with the larynx being the most common site. To date, nearly 700 cases of laryngeal neuroendocrine carcinoma (NEC) have been reported in the literature, with an estimated incidence as low as 0.23%. This type of cancer is more prevalent among men aged 50–83 who are heavy smokers. NENs encompass paragangliomas and epithelial neoplasms. The latter categories include neuroendocrine tumors, or typical carcinoids, and NECs, or atypical carcinoids. Due to their nonspecific and often misleading presentation, and given the rarity of this condition, optimal management lacks standardization. Treatment typically involves a combination of surgery, chemotherapy, and radiotherapy. We present a case of supraglottic laryngeal NEC in a 61‐year‐old female nonsmoker. The patient underwent endoscopic excision followed by adjuvant radiotherapy.
Construction and Validation of a Risk Prediction Model for Colon Adenocarcinoma Prognosis Based on 11 Depression‐Related Genes
Authors: Yuan Yunchuan , Xia Lili , Wu Xintong , Yang Hongjing , Dou Lu
Published: October 2024

Background: The influences of depression on cancer have noticeably attracted scholars’ attention. This study is aimed at exploring the relationships between depression and colon adenocarcinoma (COAD).

Methods: Differentially expressed genes in COAD were overlapped with depression‐related genes (DRGs) to obtain COAD‐DRGs. A risk model was constructed to predict overall survival (OS) using univariate and multivariate Cox regression analyses. GSE39582 dataset was utilized to validate the model. A nomogram was developed utilizing the clinical data.

Results: A risk model containing 11 genes was constructed. The results of receiver operating characteristic curve analysis revealed that the model could well predict the OS. In the high‐risk group, the infiltration levels of plasma cells, resting/activated memory CD4 T cells, and monocytes were reduced, and only the infiltration levels of CD8 T cells and regulatory T cells were elevated. Cox regression analysis indicated that the risk score emerged as an independent prognostic factor. Finally, a nomogram of comprehensive risk score, age, and pM stage was established, and the predictions of this model aligned well with the actual OS data.

Conclusion: A COAD risk prediction model was successfully constructed utilizing 11 DRGs. This model assists in implementing more effective treatment and care strategies, enhancing the clinical outcomes for COAD.
Periodic and Antiperiodic Solutions for a Second‐Order Hamiltonian System With Nonlinearity Depending on Derivative
Authors: Chen Wenxiong , Liu Shibo
Published: October 2024

Some existence results of periodic solution are obtained for a class of second‐order Hamiltonian systems with nonlinearity depending on derivative. We prove that there exists T0 > 0 such that, for any T < T0, the provided Hamiltonian system has a nontrivial T‐periodic and T/2‐antiperiodic solution via linking theorem and iteration method.
Prevalence and Risk Factors of Postpartum Depression Among Women in Low‐Income Developing Rural Areas: A Cross‐Sectional Study in China
Authors: Sun Mei , Cao Fanfan , Peng Jiayuan , Tang Jingfei , He Yuqing , Zeng Yi , Tan Xiangmin , Zhao Qian
Published: October 2024

Background: Postpartum depression (PPD) significantly affects the welfare of mothers, infants, families, and communities. Mothers in rural areas often face low incomes, poor social security, low education levels, and inadequate medical services. These specific cultural, social, and economic aspects have led to a worsening of PPD in rural areas. However, the current situation of PPD among women in rural areas of China is still insufficiently explored.

Aim: This study aims to explore the prevalence and risk factors of PPD among women in low‐income developing rural areas of China.

Methods: A cross‐sectional design was used in this study. Edinburgh Postnatal Depression Scale (EPDS) was applied to evaluate PPD symptoms. General demographic questionnaire, obstetrics‐/pediatrics‐related questionnaire, and psychosocial‐related questionnaire were adopted. Abuse Assessment Screen (AAS) was utilized to assess experienced intimate partner violence during pregnancy and postpartum. Social Support Rating Scale (SSRS) was utilized to measure their levels of social support.

Results: Of the 467 participants, the overall prevalence of PPD among women in rural areas of China was 16.5%, and the average EPDS score was 8.35 (SD = 4.50). PPD occurred most frequently at 7–9 months postpartum (33.8%). Six factors associated with PPD were whether the sex of the baby was in line with the family’s expectations, monthly income of partners, social support, IPV during pregnancy and childbirth, and negative life events in the last 1 year, as well as physical and mental exhaustion from caring for a baby.

Conclusions: This study sheds light on the prevalence and various risk factors associated with PPD among women residing in low‐income developing rural areas of China. The findings highlighted the need for targeted interventions and support systems designed to address the specific socioeconomic and cultural difficulties encountered by rural mothers.
Supplementary Figure 8 from A Prime-Boost Vaccination Approach Induces Lung Resident Memory CD8+ T Cells Derived from Central Memory T Cells That Prevent Tumor Lung Metastasis
Authors: Xu Haoran , Yue Ming , Zhou Runhong , Wang Pui , Wong Michael Yik-Chun , Wang Jinlin , Huang Huarong , Chen Bohao , Mo Yufei , Tam Rachel Chun-Yee , Zhou Biao , Du Zhenglong , Huang Haode , Liu Li , Tan Zhiwu , Yuen Kwok-Yung , Song Youqiang , Chen Honglin , Chen Zhiwei
Published: October 2024

Supplementary Figure 8
Supplementary Figure 6 from A Prime-Boost Vaccination Approach Induces Lung Resident Memory CD8+ T Cells Derived from Central Memory T Cells That Prevent Tumor Lung Metastasis
Authors: Xu Haoran , Yue Ming , Zhou Runhong , Wang Pui , Wong Michael Yik-Chun , Wang Jinlin , Huang Huarong , Chen Bohao , Mo Yufei , Tam Rachel Chun-Yee , Zhou Biao , Du Zhenglong , Huang Haode , Liu Li , Tan Zhiwu , Yuen Kwok-Yung , Song Youqiang , Chen Honglin , Chen Zhiwei
Published: October 2024

Supplementary Figure 6
Supplementary Figure 10 from A Prime-Boost Vaccination Approach Induces Lung Resident Memory CD8+ T Cells Derived from Central Memory T Cells That Prevent Tumor Lung Metastasis
Authors: Xu Haoran , Yue Ming , Zhou Runhong , Wang Pui , Wong Michael Yik-Chun , Wang Jinlin , Huang Huarong , Chen Bohao , Mo Yufei , Tam Rachel Chun-Yee , Zhou Biao , Du Zhenglong , Huang Haode , Liu Li , Tan Zhiwu , Yuen Kwok-Yung , Song Youqiang , Chen Honglin , Chen Zhiwei
Published: October 2024

Kinetic changes of CD8+ T subclusters and other subpopulations in response to different immunization approaches
Supplementary Data from Multidimensional Fragmentomics Enables Early and Accurate Detection of Colorectal Cancer
Authors: Cao Yuepeng , Wang Nannan , Wu Xuxiaochen , Tang Wanxiangfu , Bao Hua , Si Chengshuai , Shao Peng , Li Dongzheng , Zhou Xin , Zhu Dongqin , Yang Shanshan , Wang Fufeng , Su Guoqing , Wang Ke , Wang Qifan , Zhang Yao , Wang Qiangcheng , Yu Dongsheng , Jiang Qian , Bao Jun , Yang Liu
Published: October 2024

Supplementary Data
Supplementary Materials and Methods from NAT10 Phase Separation Regulates YTHDF1 Splicing to Promote Gastric Cancer Progression
Authors: Liu Songyi , Lin Chunlin , Lin Xiang , Lin Penghang , He Ruofan , Pan Xiaoyu , Lin Yan , Ye Jianxin , Zhu Guangwei
Published: October 2024

Supplementary Materials and Methods
Supplementary Data from Integrative Modeling of Signaling Network Dynamics Identifies Cell Type–Selective Therapeutic Strategies for FGFR4-Driven Cancers
Authors: Shin Sung-Young , Chew Nicole J. , Ghomlaghi Milad , Chüeh Anderly C. , Jeong Yunhui , Nguyen Lan K. , Daly Roger J.
Published: October 2024

Supplementary Notes, Supplementary Figures, and Supplementary Figure Legends
Supplementary Data S5 from Integrative Modeling of Signaling Network Dynamics Identifies Cell Type–Selective Therapeutic Strategies for FGFR4-Driven Cancers
Authors: Shin Sung-Young , Chew Nicole J. , Ghomlaghi Milad , Chüeh Anderly C. , Jeong Yunhui , Nguyen Lan K. , Daly Roger J.
Published: October 2024

Supplementary Data S5
Supplementary Data S3 from Integrative Modeling of Signaling Network Dynamics Identifies Cell Type–Selective Therapeutic Strategies for FGFR4-Driven Cancers
Authors: Shin Sung-Young , Chew Nicole J. , Ghomlaghi Milad , Chüeh Anderly C. , Jeong Yunhui , Nguyen Lan K. , Daly Roger J.
Published: October 2024

Supplementary Data S3
Table S1 from Proteomic Profiling of Serum Extracellular Vesicles Identifies Diagnostic Signatures and Therapeutic Targets in Breast Cancer
Authors: Xu Ganfei , Huang Rui , Wumaier Reziya , Lyu Jiacheng , Huang Minjing , Zhang Yaya , Chen Qingjian , Liu Wenting , Tao Mengyu , Li Junjian , Tao Zhonghua , Yu Bo , Xu Erxiang , Wang Lingfeng , Yu Guoying , Gires Olivier , Zhou Lei , Zhu Wei , Ding Chen , Wang Hongxia
Published: October 2024

The clinical data for our discovery cohort and validation cohorts. Related to Figure 1.
Figure S5 from Proteomic Profiling of Serum Extracellular Vesicles Identifies Diagnostic Signatures and Therapeutic Targets in Breast Cancer
Authors: Xu Ganfei , Huang Rui , Wumaier Reziya , Lyu Jiacheng , Huang Minjing , Zhang Yaya , Chen Qingjian , Liu Wenting , Tao Mengyu , Li Junjian , Tao Zhonghua , Yu Bo , Xu Erxiang , Wang Lingfeng , Yu Guoying , Gires Olivier , Zhou Lei , Zhu Wei , Ding Chen , Wang Hongxia
Published: October 2024

Potential EV survival biomarkers for the distant metastases of BC, related to Figure 5
Data from Integrative Modeling of Signaling Network Dynamics Identifies Cell Type–Selective Therapeutic Strategies for FGFR4-Driven Cancers
Authors: Shin Sung-Young , Chew Nicole J. , Ghomlaghi Milad , Chüeh Anderly C. , Jeong Yunhui , Nguyen Lan K. , Daly Roger J.
Published: October 2024

Abstract
Oncogenic FGFR4 signaling represents a potential therapeutic target in various cancer types, including triple-negative breast cancer and hepatocellular carcinoma. However, resistance to FGFR4 single-agent therapy remains a major challenge, emphasizing the need for effective combinatorial treatments. Our study sought to develop a comprehensive computational model of FGFR4 signaling and to provide network-level insights into resistance mechanisms driven by signaling dynamics. An integrated approach, combining computational network modeling with experimental validation, uncovered potent AKT reactivation following FGFR4 targeting in triple-negative breast cancer cells. Analyzing the effects of cotargeting specific network nodes by systematically simulating the model predicted synergy of cotargeting FGFR4 and AKT or specific ErbB kinases, which was subsequently confirmed through experimental validation; however, cotargeting FGFR4 and PI3K was not synergistic. Protein expression data from hundreds of cancer cell lines was incorporated to adapt the model to diverse cellular contexts. This revealed that although AKT rebound was common, it was not a general phenomenon. For example, ERK reactivation occurred in certain cell types, including an FGFR4-driven hepatocellular carcinoma cell line, in which there is a synergistic effect of cotargeting FGFR4 and MEK but not AKT. In summary, this study offers key insights into drug-induced network remodeling and the role of protein expression heterogeneity in targeted therapy responses. These findings underscore the utility of computational network modeling for designing cell type–selective combination therapies and enhancing precision cancer treatment.

 Significance: Computational predictive modeling of signaling networks can decipher mechanisms of cancer cell resistance to targeted therapies and enable identification of more effective cancer type–specific combination treatment strategies.
Supplementary Figure S16 from Transcriptional Phenocopies of Deleterious KEAP1 Mutations Correlate with Survival Outcomes in Lung Cancer Treated with Immunotherapy
Authors: Scalera Stefano , Ricciuti Biagio , Marinelli Daniele , Mazzotta Marco , Cipriani Laura , Bon Giulia , Schiavoni Giulia , Terrenato Irene , Di Federico Alessandro , Alessi Joao V. , Fanciulli Maurizio , Ciuffreda Ludovica , De Nicola Francesca , Goeman Frauke , Caravagna Giulio , Santini Daniele , De Maria Ruggero , Cappuzzo Federico , Ciliberto Gennaro , Jamal-Hanjani Mariam , Awad Mark M. , McGranahan Nicholas , Maugeri-Saccà Marcello
Published: October 2024

Supplementary Figure 16. Impact of the KEAPness-dominant phenotype according to tumor histology in the pooled SU2C/OAK/POPLAR cohort.
Supplemental Figure S5 from Genomic Profiling to Contextualize the Results of Intervention for Smoldering Multiple Myeloma
Authors: Kazandjian Dickran , Diamond Benjamin , Papadimitriou Marios , Hill Elizabeth , Sklavenitis-Pistofidis Romanos , Ziccheddu Bachisio , Blaney Patrick , Chojnacka Monika , Durante Michael , Maclachlan Kylee , Young Ryan , Usmani Saad , Davies Faith , Getz Gad , Ghobrial Irene , Korde Neha , Morgan Gareth , Maura Francesco , Landgren Ola
Published: October 2024

Supplemental Figure S5. Structural Variant Hotspots from WGS Cases.
Supplemental Figure S4 from Genomic Profiling to Contextualize the Results of Intervention for Smoldering Multiple Myeloma
Authors: Kazandjian Dickran , Diamond Benjamin , Papadimitriou Marios , Hill Elizabeth , Sklavenitis-Pistofidis Romanos , Ziccheddu Bachisio , Blaney Patrick , Chojnacka Monika , Durante Michael , Maclachlan Kylee , Young Ryan , Usmani Saad , Davies Faith , Getz Gad , Ghobrial Irene , Korde Neha , Morgan Gareth , Maura Francesco , Landgren Ola
Published: October 2024

Supplemental Figure S4. Genomic Features Comparing HR-SMM and NDMM.
Supplementary Figure S2 from Targeting the Dendritic Cell-Secreted Immunoregulatory Cytokine CCL22 Alleviates Radioresistance
Authors: Bugno Jason , Wang Liangliang , Yu Xianbin , Cao Xuezhi , Wang Jiaai , Huang Xiaona , Yang Kaiting , Piffko Andras , Chen Katherine , Luo Stephen Y. , Naccasha Emile , Hou Yuzhu , Fu Sherry , He Chuan , Fu Yang-xin , Liang Hua L. , Weichselbaum Ralph R.
Published: October 2024

Supplementary Figure S2. Flow cytometric characterization of tumor cDC after local radiation
Supplementary Figure S4 from Spatial Transcriptome Mapping of the Desmoplastic Growth Pattern of Colorectal Liver Metastases by In Situ Sequencing Reveals a Biologically Relevant Zonation of the Desmoplastic Rim
Authors: Andersson Axel , Escriva Conde Maria , Surova Olga , Vermeulen Peter , Wählby Carolina , Nilsson Mats , Nyström Hanna
Published: October 2024

Supplementary Figure S4. Cluster concentration as a function of distance from liver and tumor. Clusters 0 to 2 and Cluster 8 show, respectively, a bias towards the tumor interface (inner rim) and liver interface (outer rim).
Supplementary Figure S10 from Pharmacologic Inhibition of EIF4A Blocks NRF2 Synthesis to Prevent Osteosarcoma Metastasis
Authors: Lizardo Michael M. , Hughes Christopher , Huang Yue Z. , Shyp Taras , Delaidelli Alberto , Zhang Hai-Feng , Shaool Sol Snir , Renner Annalena F. , Burwag Farez , Sayles Leanne C. , Lee Alex G. , Sweet-Cordero Alejandro , Sorensen Poul H.
Published: October 2024

Fig. S10. CR-1-31B down-modulates NRF2 expression in xenograft primary tumor and lung metastases
Supplementary Table S3 from Tyrosine Protein Kinase SYK-Related Gene Signature in Baseline Immune Cells Associated with Adjuvant Immunotherapy–Induced Immune-Related Adverse Events in Melanoma
Authors: Monson Kelsey R. , Ferguson Robert , Handzlik Joanna E. , Xiong Jiahan , Dagayev Sasha , Morales Leah , Chat Vylyny , Bunis Anabelle , Sreenivasaiah Chaitra , Dolfi Sonia , Tenney Daniel J. , Shao Yongzhao , Osman Iman , Weber Jeffrey S. , Kirchhoff Tomas
Published: October 2024

Supplementary Table S3. GO analysis of differentially expressed genes identified in the study.
Supplementary Figure S5 from Tyrosine Protein Kinase SYK-Related Gene Signature in Baseline Immune Cells Associated with Adjuvant Immunotherapy–Induced Immune-Related Adverse Events in Melanoma
Authors: Monson Kelsey R. , Ferguson Robert , Handzlik Joanna E. , Xiong Jiahan , Dagayev Sasha , Morales Leah , Chat Vylyny , Bunis Anabelle , Sreenivasaiah Chaitra , Dolfi Sonia , Tenney Daniel J. , Shao Yongzhao , Osman Iman , Weber Jeffrey S. , Kirchhoff Tomas
Published: October 2024

Supplementary Figure S5. Detailed annotation for semi-supervised hierarchical clustering of the genes associated with SYK in protein-protein interaction (PPI) analysis comparing TOXlowvs TOXhighin CD4+cells from COMBO-treated patients (n = 78).
Supplementary Figure 4 from Colorectal Cancer Recurrence Prediction Using a Tissue-Free Epigenomic Minimal Residual Disease Assay
Authors: Nakamura Yoshiaki , Tsukada Yuichiro , Matsuhashi Nobuhisa , Murano Tatsuro , Shiozawa Manabu , Takahashi Yusuke , Oki Eiji , Goto Masahiro , Kagawa Yoshinori , Kanazawa Akiyoshi , Ohta Takashi , Ouchi Akira , Bando Hideaki , Uchigata Hiroshi , Notake Chiemi , Ikematsu Hiroaki , Yoshino Takayuki
Published: October 2024

Supplementary Figure 4. ctDNA kinetics among 15 recurrence patients with more than one positive sample.
Supplementary Table S5 from FDA Approval Summary: Asciminib for Ph+ CML in Chronic Phase Treated with Two or More Tyrosine Kinase Inhibitors and for the T315I Mutation
Authors: Pamuk Gulsum E. , Chow Edwin C.Y. , Ionan Alexei C. , Chen Haiyan , Lee Shwu-Luan , Hsu Vicky , Grimstein Manuela , Zheng Nan , Sun Jielin , Charlab Rosane , Gehrke Brenda J. , Vallejo Jonathon , Ehrlich Lori A. , de Claro R. Angelo , Theoret Marc R.
Published: October 2024

Select laboratory abnormalities (≥ 10%) that worsened from baseline in patients with Ph + CML in CP with the T315I mutation in X2101 trial
Supplementary Figure S8 from Clinical Value of Liquid Biopsy in Patients with FGFR2 Fusion–Positive Cholangiocarcinoma During Targeted Therapy
Authors: González-Medina Alberto , Vila-Casadesús Maria , Gomez-Rey Marina , Fabregat-Franco Carles , Sierra Alexandre , Tian Tian V. , Castet Florian , Castillo Gloria , Matito Judit , Martinez Paola , Miquel Josep M. , Nuciforo Paolo , Pérez-López Raquel , Macarulla Teresa , Vivancos Ana
Published: October 2024

Supplementary Figure S8. Relationship between plasma biomarkers and liver damage.
Table 2 from Elacestrant in ER+, HER2− Metastatic Breast Cancer with ESR1-Mutated Tumors: Subgroup Analyses from the Phase III EMERALD Trial by Prior Duration of Endocrine Therapy plus CDK4/6 Inhibitor and in Clinical Subgroups
Authors: Bardia Aditya , Cortés Javier , Bidard François-Clément , Neven Patrick , Garcia-Sáenz José , Aftimos Phillipe , O’Shaughnessy Joyce , Lu Janice , Tonini Giulia , Scartoni Simona , Paoli Alessandro , Binaschi Monica , Wasserman Tomer , Kaklamani Virginia
Published: October 2024

PFS in subgroups of patients with ESR1-mutated tumors and prior ET+CDK4/6i ≥12 months.
Figure 2 from Elacestrant in ER+, HER2− Metastatic Breast Cancer with ESR1-Mutated Tumors: Subgroup Analyses from the Phase III EMERALD Trial by Prior Duration of Endocrine Therapy plus CDK4/6 Inhibitor and in Clinical Subgroups
Authors: Bardia Aditya , Cortés Javier , Bidard François-Clément , Neven Patrick , Garcia-Sáenz José , Aftimos Phillipe , O’Shaughnessy Joyce , Lu Janice , Tonini Giulia , Scartoni Simona , Paoli Alessandro , Binaschi Monica , Wasserman Tomer , Kaklamani Virginia
Published: October 2024

PFS according to clinical subgroups. Kaplan–Meier estimates of PFS in patients with ESR1-mutated tumors and prior ET+CDK4/6i ≥12 months who, at screening, had the presence of (A) bone metastases (n = 136; 86%); (B) liver and/or lung metastases (n = 113; 71%); (C) <3 metastatic sites (n = 82; 52%); or (D) ≥3 metastatic sites (n = 53; 33%). aEighty-five percent of patients had bone and other sites of metastases (30% of these patients had no liver or lung involvement). bFifty-five percent of patients had liver and other sites of metastases (10% of these patients had no lung or bone involvement); 25% of patients had lung and other sites of metastases (2% of these patients had no liver or bone involvement). cThe number of metastatic sites was available for 135 of 159 patients with ESR1-mutated tumors and prior ET+CDK4/6i ≥12 months.
Data from Spatial Transcriptome Mapping of the Desmoplastic Growth Pattern of Colorectal Liver Metastases by In Situ Sequencing Reveals a Biologically Relevant Zonation of the Desmoplastic Rim
Authors: Andersson Axel , Escriva Conde Maria , Surova Olga , Vermeulen Peter , Wählby Carolina , Nilsson Mats , Nyström Hanna
Published: October 2024

AbstractPurpose:
We describe the fibrotic rim formed in the desmoplastic histopathologic growth pattern (DHGP) of colorectal cancer liver metastasis (CLM) using in situ sequencing (ISS). The origin of the desmoplastic rim is still a matter of debate, and the detailed cellular organization has not yet been fully elucidated. Understanding the biology of the DHGP in CLM can lead to targeted treatment and improve survival.
Experimental Design:
We used ISS, targeting 150 genes, to characterize the desmoplastic rim by unsupervised clustering of gene coexpression patterns. The cohort comprised 10 chemo-naïve liver metastasis resection samples with a DHGP.
Results:
Unsupervised clustering of spatially mapped genes revealed molecular and cellular diversity within the desmoplastic rim. We confirmed the presence of the ductular reaction and cancer-associated fibroblasts. Importantly, we discovered angiogenesis and outer and inner zonation in the rim, characterized by nerve growth factor receptor and periostin expression.
Conclusions:
ISS enabled the analysis of the cellular organization of the fibrous rim surrounding CLM with a DHGP and suggests a transition from the outer part of the rim, with nonspecific liver injury response, into the inner part, with gene expression indicating collagen synthesis and extracellular matrix remodeling influenced by the interaction with cancer cells, creating a cancer cell–supportive environment. Moreover, we found angiogenic processes in the rim. Our results provide a potential explanation of the origin of the rim in DHGP and lead to exploring novel targeted treatments for patients with CLM to improve survival.
Table.S1 from FZ-AD005, a Novel DLL3-Targeted Antibody–Drug Conjugate with Topoisomerase I Inhibitor, Shows Potent Antitumor Activity in Preclinical Models
Authors: Guo Qingsong , Gao Bei , Song Ruiwen , Li Weinan , Zhu Shulei , Xie Qian , Lou Sensen , Wang Lei , Shen Jiafei , Zhao Teng , Zhang Yifan , Wu Jinsong , Lu Wei , Yang Tong
Published: October 2024

Supplementary Table.S1
Figure S4 from PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER+ Breast Cancer
Authors: Chen Anran , Kim Beom-Jun , Mitra Aparna , Vollert Craig T. , Lei Jonathan T. , Fandino Diana , Anurag Meenakshi , Holt Matthew V. , Gou Xuxu , Pilcher Jacob B. , Goetz Matthew P. , Northfelt Donald W. , Hilsenbeck Susan G. , Marshall C. Gary , Hyer Marc L. , Papp Robert , Yin Shou-Yun , De Angelis Carmine , Schiff Rachel , Fuqua Suzanne A.W. , Ma Cynthia X. , Foulds Charles E. , Ellis Matthew J.
Published: October 2024

PKMYT1 mRNA significantly correlates with PKMYT1 protein
Figure S16 from PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER+ Breast Cancer
Authors: Chen Anran , Kim Beom-Jun , Mitra Aparna , Vollert Craig T. , Lei Jonathan T. , Fandino Diana , Anurag Meenakshi , Holt Matthew V. , Gou Xuxu , Pilcher Jacob B. , Goetz Matthew P. , Northfelt Donald W. , Hilsenbeck Susan G. , Marshall C. Gary , Hyer Marc L. , Papp Robert , Yin Shou-Yun , De Angelis Carmine , Schiff Rachel , Fuqua Suzanne A.W. , Ma Cynthia X. , Foulds Charles E. , Ellis Matthew J.
Published: October 2024

Effect of knocking down mutant p53 and re-expressing wild-type (WT) p53 in T47D Palbo-R cells on synergy and viability to the RP-6306 and gemcitabine combination
Figure S15 from PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER+ Breast Cancer
Authors: Chen Anran , Kim Beom-Jun , Mitra Aparna , Vollert Craig T. , Lei Jonathan T. , Fandino Diana , Anurag Meenakshi , Holt Matthew V. , Gou Xuxu , Pilcher Jacob B. , Goetz Matthew P. , Northfelt Donald W. , Hilsenbeck Susan G. , Marshall C. Gary , Hyer Marc L. , Papp Robert , Yin Shou-Yun , De Angelis Carmine , Schiff Rachel , Fuqua Suzanne A.W. , Ma Cynthia X. , Foulds Charles E. , Ellis Matthew J.
Published: October 2024

Immunoblotting of MCF7 EDR and EDR Palbo-R cell lines to validate p53 protein knockdown
Supplementary Figure 1 from The Vedotin Antibody–Drug Conjugate Payload Drives Platform-Based Nonclinical Safety and Pharmacokinetic Profiles
Authors: Neff-LaFord Haley D. , Carratt Sarah A. , Carosino Christopher , Everds Nancy , Cardinal Kristen A. , Duniho Steven , Schutten Melissa M. , Frantz Christopher , Zuch de Zafra Christina , Harstad Eric B.
Published: October 2024

Supplementary Figure 1. Hematopoietic and lymphoid tissue toxicity in cynomolgus monkeys administered a vedotin ADC or MMAE.
Supplementary Figure S5 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment
Authors: Chen Chang-Jung , Wang Hao-Chen , Hou Ya-Chin , Wu Yi-Ying , Shieh Chi-Chang , Shan Yan-Shen
Published: October 2024

Supplementary Figure S5. An inverse correlation between HDAC2 and CYBB mRNA expression after IL-4 treatments in U937 cells. RT-qPCR analysis was performed to measure HDAC2 and CYBB mRNA expression in U937 cells after treatment with IL-4 (20 ng/ml) at different times. The bar graphs depict the relative mRNA expression. *, p < 0.05; **, p < 0.01; ***, p < 0.001, compared with control group. Data represent the mean of 3 independent experiments.
Table S6 from AKT Inhibition Sensitizes to Polo-Like Kinase 1 Inhibitor Onvansertib in Prostate Cancer
Authors: Nouri Mannan , Varkaris Andreas , Ridinger Maya , Dalrymple Susan L. , Dennehy Christopher M. , Isaacs John T. , Einstein David J. , Brennen W.N. , Balk Steven P.
Published: October 2024

Table S6 (% of Control)
Supplementary Figure S1 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment
Authors: Chen Chang-Jung , Wang Hao-Chen , Hou Ya-Chin , Wu Yi-Ying , Shieh Chi-Chang , Shan Yan-Shen
Published: October 2024

Supplementary Figure S1. High CD204 and low CYBB expression are associated with poor survival in pancreatic cancer patients. A, Immunofluorescence (IF) staining for CYBB expression was performed on tissue microarrays of pancreatic cancer. White arrows indicate CYBB-positive cells. DAPI was used for staining cell nuclei (blue). B and C, Kaplan-Meier survival analysis was performed to evaluate the association of CD204 expression with OS and DFS in pancreatic cancer patients. D and E, Kaplan-Meier survival analysis was performed to evaluate the association of CYBB expression with OS and DFS in pancreatic cancer patients.
Table S1 from AKT Inhibition Sensitizes to Polo-Like Kinase 1 Inhibitor Onvansertib in Prostate Cancer
Authors: Nouri Mannan , Varkaris Andreas , Ridinger Maya , Dalrymple Susan L. , Dennehy Christopher M. , Isaacs John T. , Einstein David J. , Brennen W.N. , Balk Steven P.
Published: October 2024

Table S1 (Viability)
Figure S8 from AKT Inhibition Sensitizes to Polo-Like Kinase 1 Inhibitor Onvansertib in Prostate Cancer
Authors: Nouri Mannan , Varkaris Andreas , Ridinger Maya , Dalrymple Susan L. , Dennehy Christopher M. , Isaacs John T. , Einstein David J. , Brennen W.N. , Balk Steven P.
Published: October 2024

Combination onvansertib and ipatasertib increases apoptosis.
Supplementary Table S4 from Discovery of Monovalent Direct Degraders of BRD4 that Act via the Recruitment of DCAF11
Authors: Parker Gregory S. , Toth Julia I. , Fish Sarah , Blanco Gabrielle , Kampert Taylor , Li Xiaoming , Yang Linette , Stumpf Craig R. , Steadman Kenneth , Jamborcic Aleksandar , Chien Stephen , Daniele Elizabeth , Dearie Alejandro , Leriche Geoffray , Bailey Simon , Thompson Peggy A.
Published: October 2024

Supplementary Table S4 shows information for the antibodies used in these studies
Supplementary Figure S6 from The SOS1 Inhibitor MRTX0902 Blocks KRAS Activation and Demonstrates Antitumor Activity in Cancers Dependent on KRAS Nucleotide Loading
Authors: Sudhakar Niranjan , Yan Larry , Qiryaqos Fadia , Engstrom Lars D. , Laguer Jade , Calinisan Andrew , Hebbert Allan , Waters Laura , Moya Krystal , Bowcut Vickie , Vegar Laura , Ketcham John M. , Ivetac Anthony , Smith Christopher R. , Lawson J. David , Rahbaek Lisa , Clarine Jeffrey , Nguyen Natalie , Saechao Barbara , Parker Cody , Elliott Adam J. , Vanderpool Darin , He Leo , Hover Laura D. , Fernandez-Banet Julio , Coma Silvia , Pachter Jonathan A. , Hallin Jill , Marx Matthew A. , Briere David M. , Christensen James G. , Olson Peter , Haling Jacob , Khare Shilpi
Published: October 2024

Supplementary Figure S6 details the improved antiproliferative activity observed with the MRTX0902/osimertinib combination in EGFR mutant models, PC9 (EGFR ex19del) and NCI-H1975 (EGFR L858R/T790M).
Supplementary Figure S4 from The SOS1 Inhibitor MRTX0902 Blocks KRAS Activation and Demonstrates Antitumor Activity in Cancers Dependent on KRAS Nucleotide Loading
Authors: Sudhakar Niranjan , Yan Larry , Qiryaqos Fadia , Engstrom Lars D. , Laguer Jade , Calinisan Andrew , Hebbert Allan , Waters Laura , Moya Krystal , Bowcut Vickie , Vegar Laura , Ketcham John M. , Ivetac Anthony , Smith Christopher R. , Lawson J. David , Rahbaek Lisa , Clarine Jeffrey , Nguyen Natalie , Saechao Barbara , Parker Cody , Elliott Adam J. , Vanderpool Darin , He Leo , Hover Laura D. , Fernandez-Banet Julio , Coma Silvia , Pachter Jonathan A. , Hallin Jill , Marx Matthew A. , Briere David M. , Christensen James G. , Olson Peter , Haling Jacob , Khare Shilpi
Published: October 2024

Supplementary Figure S4 shows levels of KRAS-MAPK pathway modulation associated with coadministration of MRTX0902 and adagrasib in the MIA PaCa-2 (KRAS G12C-mutant) model.
Data from Anti-Claudin-2 Antibody–Drug Conjugates for the Treatment of Colorectal Cancer Liver Metastasis
Authors: Tabariès Sébastien , Robert Alma , Marcil Anne , Ling Binbing , Acchione Mauro , Lippens Julie , Pagé Martine , Fortin Annie , Meury Luc , Coutu Mathieu , Annis Matthew G. , Girondel Charlotte , Navarre Julie , Jaramillo Maria , Moraitis Anna N. , Siegel Peter M.
Published: October 2024

Abstract
We have previously demonstrated that Claudin-2 is required for colorectal cancer (CRC) liver metastasis. The expression of Claudin-2 in primary CRC is associated with poor survival and highly expressed in liver metastases. Claudin-2 also promotes breast cancer liver metastasis by enabling seeding and cancer cell survival. These observations support Claudin-2 as a potential therapeutic target for managing patients with liver metastases. Antibody–drug conjugates (ADC) are promising antitumor therapeutics, which combine the specific targeting ability of monoclonal antibodies with the potent cell killing activity of cytotoxic drugs. Herein, we report the generation of 28 anti-Claudin-2 antibodies for which the binding specificities, cross-reactivity with claudin family members, and cross-species reactivity were assessed by flow cytometry analysis. Multiple drug conjugates were tested, and PNU was selected for conjugation with anti-Claudin-2 antibodies binding either extracellular loop 1 or 2. Anti-Claudin-2 ADCs were efficiently internalized and were effective at killing Claudin-2-expressing CRC cancer cells in vitro. Importantly, PNU-conjugated-anti-Claudin-2 ADCs impaired the development of replacement-type CRC liver metastases in vivo, using established CRC cell lines and patient-derived xenograft (PDX) models of CRC liver metastases. Results suggest that the development of ADCs targeting Claudin-2 is a promising therapeutic strategy for managing patients with CRC liver-metastatic disease who present replacement-type liver metastases.
Supplementary Figure S3 from Cancer History and Social Support Impact Colorectal Cancer Screening Utilization by Race/Ethnicity
Authors: Tsai Meng-Han , Lee Minjee , Coughlin Steven S. , Eberth Jan M. , Rogers Charles R.
Published: October 2024

Supplementary Figure S3 showsed the association between race/ethnicity, cancer history, lack of social support, and CRC screening utilization adjusted for sociodemographic characteristics
Supplementary Figure S3 from Preventive Treatment with a CD73 Small Molecule Inhibitor Enhances Immune Surveillance in K-Ras Mutant Pancreatic Intraepithelial Neoplasia
Authors: Strickland Lincoln N. , Liu Wendao , Hussein Usama , Mardik Nicolette , Chen Xian , Mills Tingting , Vornik Lana A. , Savage Michelle I. , Sei Shizuko , Clifford John , Eltzschig Holger K. , Brown Powel H. , Zhao Zhongming , McAllister Florencia , Bailey-Lundberg Jennifer M.
Published: October 2024

Supplementary Figure S3. CD73 inhibitor treatment does not significantly change Granzyme B or B3GAT1 levels.
Figure S6 from NK Receptor Signaling Lowers TCR Activation Threshold, Enhancing Selective Recognition of Cancer Cells by TAA-Specific CTLs
Authors: Dong Bowen , Obermajer Nataša , Tsuji Takemasa , Matsuzaki Junko , Bonura Cindy M. , Sander Cindy , Withers Henry , Long Mark D. , Chavel Colin , Olejniczak Scott H. , Minderman Hans , Kirkwood John M. , Edwards Robert P. , Storkus Walter J. , Romero Pedro , Kalinski Pawel
Published: October 2024

DNAM-1 signaling assists TCR signaling in CTLs.
Figure S9 from Atypical B Cells Promote Cancer Progression and Poor Response to Bacillus Calmette-Guérin in Non–Muscle Invasive Bladder Cancer
Authors: Yolmo Priyanka , Rahimi Sadaf , Chenard Stephen , Conseil Gwenaëlle , Jenkins Danielle , Sachdeva Kartik , Emon Isaac , Hamilton Jake , Xu Minqi , Rangachari Manu , Michaud Eva , Mansure Jose J. , Kassouf Wassim , Berman David M. , Siemens David R. , Koti Madhuri
Published: October 2024

B‐cell depletion alters expression profiles of immune regulatory genes in the bladder microenvironment.
Figure S7 from Atypical B Cells Promote Cancer Progression and Poor Response to Bacillus Calmette-Guérin in Non–Muscle Invasive Bladder Cancer
Authors: Yolmo Priyanka , Rahimi Sadaf , Chenard Stephen , Conseil Gwenaëlle , Jenkins Danielle , Sachdeva Kartik , Emon Isaac , Hamilton Jake , Xu Minqi , Rangachari Manu , Michaud Eva , Mansure Jose J. , Kassouf Wassim , Berman David M. , Siemens David R. , Koti Madhuri
Published: October 2024

B‐cell differentiation to ABCs following in vitro treatment with IFN-γ, IL-21 and BCG is sex-dependent.
Figure S2 from NK Receptor Signaling Lowers TCR Activation Threshold, Enhancing Selective Recognition of Cancer Cells by TAA-Specific CTLs
Authors: Dong Bowen , Obermajer Nataša , Tsuji Takemasa , Matsuzaki Junko , Bonura Cindy M. , Sander Cindy , Withers Henry , Long Mark D. , Chavel Colin , Olejniczak Scott H. , Minderman Hans , Kirkwood John M. , Edwards Robert P. , Storkus Walter J. , Romero Pedro , Kalinski Pawel
Published: October 2024

Expression of DNAM-1 and DNAM-1-competing (inhibitory) receptors reflects CTL cytotoxic function.
Figure S4 from Atypical B Cells Promote Cancer Progression and Poor Response to Bacillus Calmette-Guérin in Non–Muscle Invasive Bladder Cancer
Authors: Yolmo Priyanka , Rahimi Sadaf , Chenard Stephen , Conseil Gwenaëlle , Jenkins Danielle , Sachdeva Kartik , Emon Isaac , Hamilton Jake , Xu Minqi , Rangachari Manu , Michaud Eva , Mansure Jose J. , Kassouf Wassim , Berman David M. , Siemens David R. , Koti Madhuri
Published: October 2024

Expansion of ABCs in the systemic and local bladder microenvironment.
Figure S7 from RNA-encoded Interleukin 2 with Extended Bioavailability Amplifies RNA Vaccine–Induced Antitumor T-cell Immunity
Authors: Peters Daniel , Kranz Lena M. , Eisel David , Diken Mustafa , Kreiter Sebastian , Türeci Özlem , Sahin Ugur , Vormehr Mathias
Published: October 2024

Gating strategy for antigen-specific CD8+ T cells, Tregs, and NK cells displayed in Figure 6.
Figure S11 from RNA-encoded Interleukin 2 with Extended Bioavailability Amplifies RNA Vaccine–Induced Antitumor T-cell Immunity
Authors: Peters Daniel , Kranz Lena M. , Eisel David , Diken Mustafa , Kreiter Sebastian , Türeci Özlem , Sahin Ugur , Vormehr Mathias
Published: October 2024

Representative dot plots corresponding to flow cytometry data displayed in Figure 6.
Figure S1 from RNA-encoded Interleukin 2 with Extended Bioavailability Amplifies RNA Vaccine–Induced Antitumor T-cell Immunity
Authors: Peters Daniel , Kranz Lena M. , Eisel David , Diken Mustafa , Kreiter Sebastian , Türeci Özlem , Sahin Ugur , Vormehr Mathias
Published: October 2024

Mouse body weight in response to RNA-LPX and Alb-IL2 RNA-NP or rIL-2. Mouse total body weight after each of three weekly i.v. vaccinations with OVA RNA-LPX combined with one (1x) or five (5x) i.p. injections of rIL-2 or one i.v. injection of Alb-IL2 RNA-NP or Alb RNA-NP in C57BL/6 mice (n=7 mice/group) in per cent of initial mouse body weight. Mean + SEM. Vertical lines indicate treatments (dashed: OVA RNA-LPX + Alb RNA-NP, rIL-2 or Alb-IL2 RNA-NP; dotted: repetitive rIL-2).
Supplemental Table S10 from Machine Learning–Directed Conversion of Glioblastoma Cells to Dendritic Cell–Like Antigen-Presenting Cells as Cancer Immunotherapy
Authors: Liu Tianyi , Jin Dan , Le Son B. , Chen Dongjiang , Sebastian Mathew , Riva Alberto , Liu Ruixuan , Tran David D.
Published: October 2024

scRNA-seq characteristics of iDC-APCs in the murine KR158 GBM ➝ iDC-APC conversion (supporting Main Figure 2)
Figure S4 from Targeting Tumor-Associated Sialic Acids Using Chimeric Switch Receptors Based on Siglec-9 Enhances the Antitumor Efficacy of Engineered T Cells
Authors: Eisenberg Vasyl , Hoogi Shiran , Katzman Erel , Ben Haim Nimrod , Zur-Toledano Raphaelle , Radman Maria , Reboh Yishai , Zadok Oranit , Kamer Iris , Bar Jair , Sagi Irit , Hendel Ayal , Cohen Cyrille J.
Published: October 2024

Figure S4
Supplemental Figure S14 from Machine Learning–Directed Conversion of Glioblastoma Cells to Dendritic Cell–Like Antigen-Presenting Cells as Cancer Immunotherapy
Authors: Liu Tianyi , Jin Dan , Le Son B. , Chen Dongjiang , Sebastian Mathew , Riva Alberto , Liu Ruixuan , Tran David D.
Published: October 2024

KR158-specific splenic T cells were greatly enriched in CD8+ effector T cells following ex vivo expansion and enrichment (Supporting Main Figure 6).
Supplemental Figure S1 from Machine Learning–Directed Conversion of Glioblastoma Cells to Dendritic Cell–Like Antigen-Presenting Cells as Cancer Immunotherapy
Authors: Liu Tianyi , Jin Dan , Le Son B. , Chen Dongjiang , Sebastian Mathew , Riva Alberto , Liu Ruixuan , Tran David D.
Published: October 2024

GeneRep’s performance (Supporting the Materials and Methods Section)
Supplementary Figure f1 from Intermittent MEK Inhibition with GITR Costimulation Rescues T-cell Function for Increased Efficacy with CTLA-4 Blockade in Solid Tumor Models
Authors: Dong Lauren , Choi Hyejin , Budhu Sadna , Schulze Isabell , Verma Svena , Mangarin Levi M. , Estrada Nevarro Valeria , Mehanna Nezar , Khan Jonathan F. , Venkatesh Divya , Thach Daniel , Rosen Neal , Wolchok Jedd D. , Merghoub Taha
Published: October 2024

Supplementary Figure 1. MEK inhibition with CKI27 increases MHC and checkpoint ligand expression. (A-B) Murine tumor cell lines were treated with DMSO or CKI27 for 72 hr and either with or without IFNγ (5ng/mL) for the last 24hr; n=3. FACS analysis of (A) MHC-I (H2Kb/Kd and H2Db/Dd) and MHC-II and (B) PD-L1, CD80 and CD86 surface expression. Median fluorescence intensity (MFI) values were normalized to DMSO and log transformed. Data are shown as mean±SEM.
Supplementary Figure 8 from Intermittent MEK Inhibition with GITR Costimulation Rescues T-cell Function for Increased Efficacy with CTLA-4 Blockade in Solid Tumor Models
Authors: Dong Lauren , Choi Hyejin , Budhu Sadna , Schulze Isabell , Verma Svena , Mangarin Levi M. , Estrada Nevarro Valeria , Mehanna Nezar , Khan Jonathan F. , Venkatesh Divya , Thach Daniel , Rosen Neal , Wolchok Jedd D. , Merghoub Taha
Published: October 2024

Supplementary Figure 8. Intermittent CKI27 treatment and GITR co-stimulation relieves expression of activation markers. Representative dot plot data for FACS analysis of activation markers expressed by CD8+ T cells.
Table S6 from Hypoxia-Induced Long Noncoding RNA HIF1A-AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Authors: Liao Tsai-Tsen , Chen Yu-Hsien , Li Zih-Yu , Hsiao An-Ching , Huang Ya-Li , Hao Ruo-Xin , Tai Shyh-Kuan , Chu Pen-Yuan , Shih Jing-Wen , Kung Hsing-Jien , Yang Muh-Hwa
Published: October 2024

Reagents and resources used in this study.
Supplementary Figure 6 from Intermittent MEK Inhibition with GITR Costimulation Rescues T-cell Function for Increased Efficacy with CTLA-4 Blockade in Solid Tumor Models
Authors: Dong Lauren , Choi Hyejin , Budhu Sadna , Schulze Isabell , Verma Svena , Mangarin Levi M. , Estrada Nevarro Valeria , Mehanna Nezar , Khan Jonathan F. , Venkatesh Divya , Thach Daniel , Rosen Neal , Wolchok Jedd D. , Merghoub Taha
Published: October 2024

Supplementary Figure 6. Intermittent CKI27 treatment and GITR co-stimulation relieves expression of co-inhibitory markers. (A) Gating strategy for all T cell activation assays. (B) Representative dot plot data for FACS analysis of co-inhibitory markers expressed by CD8+ T cells.
Supplementary Figure 5 from Intermittent MEK Inhibition with GITR Costimulation Rescues T-cell Function for Increased Efficacy with CTLA-4 Blockade in Solid Tumor Models
Authors: Dong Lauren , Choi Hyejin , Budhu Sadna , Schulze Isabell , Verma Svena , Mangarin Levi M. , Estrada Nevarro Valeria , Mehanna Nezar , Khan Jonathan F. , Venkatesh Divya , Thach Daniel , Rosen Neal , Wolchok Jedd D. , Merghoub Taha
Published: October 2024

Supplementary Figure 5. Intermittent CKI27 allows for immune cell recovery in the spleen, increases frequencies in the TDLN, and inhibits TILs similarly to continuous treatment. (A) Schema of LLC tumor bearing mice treated with vehicle, daily 2mg/kg CKI27, or intermittent 5mg/kg 4on/3off CKI27. Mice were treated in a staggered schedule and all timepoints were harvested on day 23. (B-D) All fold changes were calculated by normalizing to DMSO. (B) Fold changes of absolute number (cells/uL) of spleen immune cell populations. (C) Fold changes of absolute number (cells/uL) of TDLN immune cell populations. (D) Fold changes of tumor weights (mg) and TILs (cells/mg); n=4-5. Data are shown as mean±SEM. Unpaired, nonparametric Mann-Whitney test was used to compare each time point. Significance levels are indicated by asterisks (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001)
Table S2 from MR1 Gene and Protein Expression Are Enhanced by Inhibition of the Extracellular Signal-Regulated Kinase ERK
Authors: Constantin Daniel , Nosi Vladimir , Kehrer Natalie , Vacchini Alessandro , Chancellor Andrew , Contassot Emmanuel , Beshirova Aisha , Prota Gennaro , Navarini Alexander , Mori Lucia , De Libero Gennaro
Published: October 2024

Phenotype and TCR gene expression by MR1T cell clones
Supplementary Figure S5 from MR1 Gene and Protein Expression Are Enhanced by Inhibition of the Extracellular Signal-Regulated Kinase ERK
Authors: Constantin Daniel , Nosi Vladimir , Kehrer Natalie , Vacchini Alessandro , Chancellor Andrew , Contassot Emmanuel , Beshirova Aisha , Prota Gennaro , Navarini Alexander , Mori Lucia , De Libero Gennaro
Published: October 2024

Supplementary Figure S5
Supplementary Figure S6 from The E3 Ubiquitin Ligase FBXO38 Maintains the Antitumor Function of Natural Killer Cells by Sustaining IL15R Signaling
Authors: Shi Yongjing , Zheng Xiaodong , Peng Hui , Xu Chenqi , Sun Rui , Tian Zhigang , Sun Haoyu , Wang Xianwei
Published: October 2024

Supplementary Figure S6. FBXO38 deficiency increases the expression of suppressors of cell cycle program in TINK cells
Supplementary Figure S3 from The E3 Ubiquitin Ligase FBXO38 Maintains the Antitumor Function of Natural Killer Cells by Sustaining IL15R Signaling
Authors: Shi Yongjing , Zheng Xiaodong , Peng Hui , Xu Chenqi , Sun Rui , Tian Zhigang , Sun Haoyu , Wang Xianwei
Published: October 2024

Supplementary Figure S3. FBXO38 knockout does not affect spleen NK cell development and effector function