bluuebunny/crossref_metadata_2025 huggingface dataset:
Karakteristik Manajemen Sebagai Determinan Kreativitas Karyawan yang di Mediasi Kepuasan Kerja Karyawan:
Authors: Sofyan Joel Faruk , Putri Rizki Amelia , Purnama Suryari
Published: November 2022

The barrier separating employees and their co-workers or employees from their supervisors plays an essential role in maintaining an organization is going concerned because it affects employee job satisfaction and employee creativity. Although employee job satisfaction has been studied extensively, the intervening role of employee job satisfaction in the relationship between management characteristics and employee creativity is yet sparsely been researched. The present study analyses the relationships between supervisor humility and abusive supervision and employee creativity mediated by employee job satisfaction. This study was conducted using an online survey disseminated in May – June 2022, where 180 employees of the Ministry of Maritime Affairs and Fisheries located in Jakarta were selected with a purposive sampling method. The Structural Equation Model was utilized as an inferential analysis tool. The results of this study indicated that supervisor humility has a positive effect on employee job satisfaction.In contrast, supervision affects employee job satisfaction negatively. Third, employee job satisfaction has a positive impact on employee creativity. Lastly, employee job satisfaction mediates the relationship between the supervisor's humility and abusive supervision toward employee creativity.

 

Keywords: Abusive Supervisor, Employee Creativity, Employee Job Satisfaction, Supervisor Humility.

 

Abstrak

Hambatan yang memisahkan karyawan dengan rekan kerja atau karyawan dengan atasannya memegang peranan penting dalam menjaga kelangsungan hidup suatu organisasi karena mempengaruhi kepuasan kerja karyawan dan kreativitas karyawan. Meskipun kepuasan kerja karyawan telah dipelajari secara ekstensif, peran intervensi kepuasan kerja karyawan dalam hubungan antara karakteristik manajemen dan kreativitas karyawan belum banyak diteliti. Tujuan dari penelitian ini adalah untuk menganalisis hubungan antara kerendahan hati penyelia dan pengawasan yang kasar dan kreativitas karyawan yang dimediasi oleh kepuasan kerja karyawan. Penelitian ini dilakukan dengan menggunakan survei online yang disebar pada bulan Mei – Juni 2022 dimana 180 pegawai Kementerian Kelautan dan Perikanan yang berada di Jakarta dipilih dengan metode Purposive Sampling dan Structural Equation Model digunakan sebagai alat analisa inferensial. Hasil penelitian ini menunjukkan bahwa kerendahan hati penyelia berpengaruh positif terhadap kepuasan kerja karyawan. Kedua, pengawasan yang kasar berpengaruh negatif terhadap kepuasan kerja karyawan. Ketiga, kepuasan kerja karyawan berpengaruh positif terhadap kreativitas karyawan. Terakhir, kepuasan kerja karyawan memediasi hubungan antara kerendahan hati penyelia dan pengawasan yang kasar terhadap kreativitas karyawan.

 

Kata kunci: Kepuasan Kerja Karyawan, Kerendahan Hati Penyelia, Kreativitas Karyawan, Pengawasan yang Kasar.
Atrophic Body Gastritis: Clinical Presentation, Diagnosis, and Outcome
Authors: Lahner Edith , Carabotti Marilia , Annibale Bruno
Published: November 2022

Atrophic body gastritis is a chronic disorder characterised by atrophy of the oxyntic glands leading to reduced gastric acid and intrinsic factor secretion. Serological studies reported yearly prevalence and incidence rates between 3–9% and 0–11%, respectively. In atrophic body gastritis, the presence of parietal cells and/or intrinsic factor autoantibodies, and autoimmune diseases, such as autoimmune thyroid disease or Type 1 diabetes mellitus, are often observed. These cases are often diagnosed as autoimmune gastritis. This association has been included as part of the autoimmune polyendocrine syndrome. A frequent clinical presentation of atrophic body gastritis is pernicious anaemia, considered an autoimmune condition, arising from vitamin B12 malabsorption as a consequence of intrinsic factor deficiency. Another presentation may be an otherwise unexplained iron deficiency anaemia, as a result of iron malabsorption and consequence of reduced gastric acid secretion. To date, no universally accepted criteria are available to define autoimmune gastritis and to distinguish this clinical entity from chronic, Helicobacter pylori-driven, multifocal atrophic gastritis. In contrast with the classical perception of a silent condition, patients with atrophic body gastritis may complain of a spectrum of gastrointestinal symptoms, ranging from dyspepsia as early satiety, postprandial fullness, and epigastric pain, to gastro-oesophageal reflux symptoms such as regurgitation and heartburn. The timely diagnosis of atrophic body gastritis is important, as this condition puts patients at an increased risk of gastric cancer and other Type 1 carcinoids that may lead to micronutrient deficiencies crucial for erythropoiesis. The present review provides an update on epidemiological and clinical aspects as well as diagnosis and outcome of the disease.
Peran Pemerintah dan Partisipasi Masyarakat dalam Peningkatan Usaha Mikro, Kecil, dan Menengah (UMKM) pada Masa Pandemi COVID-19 di Cipageran, Cimahi
Authors: Tumija Tumija
Published: November 2022

Usaha Mikro Kecil dan Menengah (UMKM) merupakan kegiatan yang menunjang perekonomian nasional. Namun semenjak COVID-19 melanda di Indonesia, usaha pada UMKM di Kota Cimahi yang terkena dampaknya rata-rata 40%. Tujuan kegiatan pengabdian kepada masyarakat berbasis riset dan kajian ini mengetahui peran pemerintah kota Cimahi dan partisipasi masyarakat dalam meningkatkan usaha UMKM. Metode yang digunakan dalam kegiatan pengabdian kepada masyarakat berbasis riset dan kajian ini adalah Participatory Action Research (PAR). Metode ini dilakukan setelah penulis memaparkan materi,  dilanjutkan dengan partisipasi masyarakat berupa diskusi dan sharing. Hasil pembahasan kegiatan pengabdian masyarakat di Kota Cimahi bahwa peran pemerintah Kota Cimahi sudah baik sesuai dengan teori Gede Diva dan PP No. 7 Tahun 2021. Hal ini terlihat dari beberapa fasilitasi baik dari sarana dan prasarana seperti Gedung Technopark sebagai tempat pelatihan UMKM, kemudian fasilitasi pelatihan digital marketing, pelatihan SDM, pelatihan pengembangan produk. Selanjutnya peran masyarakat (pelaku UMKM) sudah aktif dalam mengikuti rapat-rapat yang diselenggarakan oleh dinas terkait dalam perencanaan program kegiatan, dan aktif dalam kegiatan-kegiatan kegiatan serta telah memanfaatkan hasil kegiatan. Dari hasil pembahasan tersebut dapat disimpulkan bahwa pemerintah Kota Cimahi telah melaksanakan tiga peran sebagai fasilitator, regulator dan katalisator dan masyarakat sudah berpartisipasi aktif dalam perencanaan, pelaksanaan dan pemanfaatan hasil dalam meningkatkan UMKM.
Sosialisasi dan Pendampingan Pembuatan Nomor Induk Berusaha (NIB) melalui Online Single Submission (OSS) kepada Pelaku Usaha Mikro di Desa Sukahayu Kecamatan Rancakalong Kabupaten Sumedang
Authors: Marthalina Marthalina , Khairina Utami
Published: November 2022

Pandemi COVID-19 telah berdampak terhadap melemahnya perekonomian masyarakat Desa Sukahayu yang ditandai dengan meningkatnya angka pengangguran. Banyak masyarakat yang mencoba peruntungan dengan membuka usaha baru yang secara administrasi masih belum memiliki izin usaha. Dari total sekitar 400 UMKM di Desa Sukahayu hanya 179 UMKM yang telah mendaftarkan izin usaha karena sebagian besar pelaku usaha menjalankan aktivitas jual beli di hari kerja, belum mampu menggunakan teknologi internet, serta belum mendapat informasi dan mengetahui urgensi pembuatan izin usaha. Sebagai bentuk upaya mengatasi permasalahan tertib administrasi di Kantor Desa Sukahayu, tim pengabdian masyarakat telah melaksanakan sosialisasi dan pendampingan secara langsung pembuatan nomor induk berusaha (NIB) melalui online single submission (OSS) di Kantor Desa Sukahayu dan secara door to door ke rumah masyarakat yang memiliki usaha mikro. Tujuan kegiatan pengabdian masyarakat ini adalah memberikan pemahaman tentang pentingnya NIB dan OSS digital untuk mendapatkan kemudahan legalitas usaha serta dokumen lainnya seperti NPWP badan atau perorangan. Kegiatan pengabdian masyarakat dilakukan dengan metode sosialisasi dan pendampingan kepada masyarakat yang dilaksanakan selama kurang lebih satu bulan serta memiliki sasaran khusus bagi masyarakat yang belum memiliki nomor induk berusaha (NIB) tetapi sudah memiliki atau menjalankan usaha mikro. Kegiatan pengabdian masyarakat ini telah berkoordinasi langsung kepada pihak pemerintahan Desa Sukahayu. Hasil dari kegiatan pengabdian masyarakat ini adalah diterbitkannya 32 nomor induk berusaha (NIB), bertambahnya pengetahuan dan informasi beberapa masyarakat tentang pentingnya nomor induk berusaha bagi para pelaku usaha mikro di Desa Sukahayu serta meningkatnya keinginan masyarakat untuk mendaftarkan usahanya secara legal yang ditandai dengan masuknya dokumen usulan baru sebanyak 17 buah. Saran masukan untuk optimalisasi pembuatan NIB di Desa Sukahayu adalah dibutuhkan komitmen dan konsistensi pemerintah Desa Sukahayu untuk melanjutkan kegiatan sosialisasi, pendampingan door to door dan pendampingan yang lebih serius bagi pelaku usaha mikro karena  masih ada kurang lebih 200 lebih usaha mikro yang belum terdaftar melalui OSS dan masih banyak masyarakat yang belum memahami pentingnya nomor induk berusaha bagi para pelaku usaha yang disebabkan asumsi masyarakat yang menganggap untuk mengurus izin dan legalitas usaha membutuhkan waktu yang panjang dan merupakan hal yang rumit. Kondisi ini sangat perlu diperhatikan dan ditindaklanjuti oleh pemerintah Desa Sukahayu dengan bekerja sama dengan Dinas terkait.
SERVİKS KANSERİ TARAMASINA BAŞVURAN KADINLARDA SAĞLIKLI YAŞAM BİÇİMİ DAVRANIŞLARI VE ETKİLEYEN FAKTÖRLER
Authors: ERTEKİN PINAR Şükran , ÖZDEMİR Sevde
Published: November 2022

Araştırma serviks kanseri taramasına başvuran kadınlarda sağlıklı yaşam biçimi davranışları ve etkileyen faktörlerin belirlenmesi amacı ile yapılmıştır. Tanımlayıcı araştırmanın örneklemini Kanser Erken Teşhis, Tarama ve Eğitim Merkezi’ne başvuran 122 kadın (30-65 yaş arası) oluşturmuştur. Veriler Kişisel Bilgi Formu ve Sağlıklı Yaşam Biçimi Davranışları Ölçeği II ile toplanmıştır. Kadınların %79.5’i menopoza girmediğini, %95.1’i ailede serviks kanseri tanısı alan bir yakını olmadığını, %86.9’u düzenli serviks kanseri taraması yaptırmadığını, ancak %96,7’si de erken tanının önemli olduğunu belirtmiştir. Lise ve üzerinde eğitim seviyesine sahip (p=0.001), sağlık (p=0.003) ve gelirini (p=0.006) iyi düzeyde algılayan, ailedeki kararlara katılan (p=0.001) ve düzenli serviks kanseri taraması yaptıran (p=0.035) kadınların Sağlıklı Yaşam Biçimi Davranışları Ölçeği II toplam puan ortalamaları arasında istatistiksel olarak anlamlı fark saptanmıştır. Sağlıklı yaşam biçimi davranışları eğitim, sağlık ve gelir düzeylerini, ailedeki kararlara katılma ve düzenli serviks kanseri taraması yaptırma durumlarını etkilemektedir. Sonuçlar düzenli serviks kanseri taraması yapılması, sağlıklı yaşam biçimi davranışlarının teşvik edilmesi ve farkındalık yaratılması konusunda sağlık profesyonellerine yol gösterici olabilir.
Investigating neural processing of syllabic and phonemic timescales in spoken language
Authors: giroud jeremy , Trébuchon-Dafonseca Agnès , Mercier Manuel , Davis Matthew , Morillon Benjamin
Published: November 2022

Recent experimental and theoretical advances in neuroscience support the idea that both low (theta) and high (gamma) frequency cortical oscillations play an important role in speech processing (Giraud and Poeppel, 2012). However, experimental evidence has better established that theta activity in the auditory cortex tracks the syllabic rhythm during speech perception. This work aims at testing the hypothesis that speech is sampled in parallel at both syllabic and phonemic timescales. A new behavioural paradigm was developed, in which independent manipulations of the number of syllabic and phonemic units were performed. Intracranial recordings from ten epileptic patients with electrodes implanted primarily in auditory regions were acquired while patients listened to these sentences. Using cerebro-acoustic coherence analyses (Peelle et al., 2012), we show that theta neural activity tracks the speech envelope and allows decoding of syllabic rhythm - while low-gamma activity tracks spectral flux allowing decoding of the rate of phonemic units.
Novel Grammatical Knowledge Causes Shifts in Both Attentional and Pre-attentive Visual Processing: ERP Evidence for Linguistic Relativity
Authors: Xue Yuyan , Williams John
Published: November 2022

It is unclear whether grammar can influence visual processing of non-linguistic stimuli in a causal manner. If yes, how deep is this effect? Here, an experimental group was trained on a semi-artificial grammar featuring two novel markers of verb transitivity, while a control group was exposed to the same materials but with the novel markers used interchangeably. After that, both groups performed two visual oddball tasks with non-linguistic stimuli that were disguised as a totally irrelevant study. The first (attentional) oddball task showed that the novel grammar cancelled an attentional bias against the dimension highlighted by the grammar (i.e. motion transitivity), as indexed by the P300 amplitude; the second (pre-attentive) oddball task showed that the grammar increased subjects’ pre-attentive response to sudden changes in motion transitivity, as indexed by the N2/visual mismatch negativity (vMMN).
An engineered hydrogel with low-dose antitumor drugs enhances tumor immunotherapy through tumor interstitial wrap
Authors: Li Zhongxian , Xiang Jiawei , Zhang Qiang , Zhao Mingyuan , Meng Yuan , Zhong Jie , Li Tingting , Jia Lanxin , Li Kai , Lu Xi , Ao Zhuo , Han Dong
Published: November 2022

Stimulating immunogenic cell death (ICD) is the key to tumor immunotherapy. However, traditional chemoradiotherapy has limited effect on stimulating immunity and often requires repeated administration, which greatly reduces the tumor-killing effect. In this article, we created a sodium alginate hydrogel sustained-release system containing low-dose doxorubicin (Dox) and immune adjuvant R837, which were injected into the interstitial space to wrap around the tumor in situ, achieving a sustained release and long-lasting immune response. Cooperating with immune checkpoint blockade, Dox induced ICD, activated dendritic cells (DCs) and converted immunosuppressive M2-type tumor-associated macrophages (TAM) to tumor-killing M1-type TAMs. Simultaneously, it greatly promoted T cell proliferation and infiltration, and reduced tumor immunosuppressive factors, triggering a robust immune response to suppress tumors in vivo. In conclusion, this anti-tumor strategy based on interstitial injection can achieve continuous local immune stimulation by low-dose chemotherapy drugs, providing a potential approach for tumor immunotherapy.
Social sustainability in construction projects: Perception versus reality and the gap-filling strategies
Authors: Kordi Nurul Elma , Belayutham Sheila , Ibrahim Che Khairil Izam Che
Published: November 2022

Past studies have recommended integrating social sustainability in the construction project to benefit a larger group of stakeholders. However, deeper insight into integrating social sustainability practices in the project remains elusive. Therefore, this study intends to address the knowledge gap by exploring the practices and perspectives of social sustainability in a construction project. The study first verified and confirmed the applicability of the social sustainability attributes adopted from the literature through structured face-to-face interviews with 15 practitioners. The practitioners have unanimously agreed that the social sustainability attributes could be categorized into nine 9) main components, namely: 1) safety and health; 2) impact assessment; 3) employment; 4) stakeholder involvement; 5) satisfaction; 6) quality education; 7) social procurement; 8) design protection/belonging; and 9) human right along with the 20 sub-attributes. The practitioners have further ranked the importance of the attributes based on real practices (experience) in their project and their professional opinion (perception). The greatest gap between the perception and practices was attributed to stakeholder involvement, indicating the need for additional effort to proactively engage stakeholders throughout the construction process. Further investigation through the gap and quadrant analyses highlighted that the education and training attribute requires immediate attention to ensure social sustainability could be practiced due to its perceived importance. This study has two major contributions: 1) provided a holistic view of the real practices against the perceived importance of social sustainability in construction projects; 2) established the gap between perception and practices of social sustainability, subsequently highlighting the strategic way forward to narrow the gap.
Oral microbiota and periodontitis severity among Hispanic adults
Authors: Ortiz Ana P. , Acosta-Pagán Kimil T. , Oramas-Sepúlveda Carla , Castañeda-Avila Maira A. , Vilanova-Cuevas Brayan , Ramos-Cartagena Jeslie M. , Vivaldi José A. , Pérez-Santiago Josué , Pérez Cynthia M. , Godoy-Vitorino Filipa
Published: November 2022

Background

Periodontitis, one of the most common bacterial infections characterized by chronic inflammation, is also known to be a risk factor for chronic conditions, including cardiovascular disease and cancer. This inflammation is driven by an altered microbiota with an increase in pathogenic bacteria. We evaluated the association between oral microbiota and periodontitis severity in high-risk Hispanics.

Method

This cross-sectional study recruited 134 sexually active participants aged 21 to 49 years old from STI Clinics in Puerto Rico. A periodontal examination, saliva collection, and an interviewer-administered questionnaire were performed. Periodontal severity was categorized as: having no disease, mild, and moderate/severe and BOP and tooth loos was noted. Saliva samples were collected for genomic DNA extraction, downstream 16S rDNA amplification sequencing, and bioinformatics analyses.

Results

The structure, composition, and diversity of bacterial communities differed significantly according to periodontal severity. The richness and overall diversity also differed between participants without periodontitis and participants with some level of periodontal disease. A higher abundance of Prevotella, Veillonella, or Treponema was attributed to periodontal disease and Aggregatibacter to severe bleeding on probing, while Neisseria was found in higher abundance in healthy participants, decreasing its levels with drinking, smoking, and oral sex practices.

Conclusions

Our findings indicate that dysbiosis occurs as periodontal disease progresses, and both alcohol consumption and smoking habits pose risk factors for oral dysbiosis. These results are of public health and clinical impact, as several bacteria identified could serve in the future as biomarkers for periodontitis and oral cancer risk.
Decision-centered design of a clinical decision support system for acute management of pediatric congenital heart disease
Authors: Assadi Azadeh , Laussen Peter C. , Freire Gabrielle , Ghassemi Marzyeh , Trbovich Patricia
Published: November 2022

Background and Objectives

Children with congenital heart disease (CHD), have fragile hemodynamics and can deteriorate due to common childhood illnesses and the natural progression of their disease. During these acute periods of deterioration, these children often present to their local emergency departments (ED) where expertise in CHD is limited, and appropriate intervention is crucial to their survival. Previous studies identified that determining the appropriate intervention for CHD patients can be difficult for ED physicians, particularly since key components of effective decision making are not being met. Although key components of effective decision making for ED physicians have been identified, they have yet to be transformed into actionable guidance. We used decision centered design (DCD) to translate key components of decision making into decision requirements and associated design concepts, that we subsequently incorporated into a prototype clinical decision support system (CDSS).

Methods

Using framework analysis, transcripts from Critical Decision Method interviews of CHD experts and ED physicians were inductively coded to identify key decision requirements for ED physicians that are currently not well supported, and their associated design concepts. A design workshop was held to refine the identified key decision requirements and design concepts as well as to sketch information that would satisfy the identified requirements. These were iteratively incorporated into a prototype CDSS.

Results

Three decision requirements: (1) distinguish the patient's unique physiology based on their unique cardiac anatomy, (2) explicitly consider CHD specific differential diagnoses to allow a more structured reflection of diagnosis, and (3) select CHD appropriate interventions for each patient, were identified. These requirements along with design concepts and information needs identified through the design workshop were incorporated into the CDSS prototype.

Conclusion

We identified key decision requirements and associated design concepts, that informed the design of a CDSS to provide actionable guidance for ED physicians when managing CHD patients. Meeting ED physicians' decision components with a CDSS requires the translation of their key decision requirements in its design. If not, we risk creating designs that interfere with clinician performance.
Structure, evolution and expression of zebrafish cartilage oligomeric matrix protein (COMP, TSP5). CRISPR-Cas mutants show a dominant phenotype in myosepta
Authors: Forte-Gomez Helena Fabiana , Gioia Roberta , Tonelli Francesca , Kobbe Birgit , Koch Peter , Bloch Wilhelm , Paulsson Mats , Zaucke Frank , Forlino Antonella , Wagener Raimund
Published: November 2022

COMP (Cartilage Oligomeric Matrix Protein), also named thrombospondin-5, is a member of the thrombospondin family of extracellular matrix proteins. It is of clinical relevance, as in humans mutations in COMP lead to chondrodysplasias. The gene encoding zebrafish Comp is located on chromosome 11 in synteny with its mammalian orthologs. Zebrafish Comp has a domain structure identical to that of tetrapod COMP and shares 74% sequence similarity with murine COMP. Zebrafish comp is expressed from 5 hours post fertilization (hpf) on, while the protein is first detectable in somites of 11 hpf embryos. During development and in adults comp is strongly expressed in myosepta, craniofacial tendon and ligaments, around ribs and vertebra, but not in its name-giving tissue cartilage. As in mammals, zebrafish Comp forms pentamers. It is easily extracted from 5 days post fertilization (dpf) whole zebrafish. The lack of Comp expression in zebrafish cartilage implies that its cartilage function evolved recently in tetrapods. The expression in tendon and myosepta may indicate a more fundamental function, as in evolutionary distant Drosophila muscle-specific adhesion to tendon cells requires thrombospondin. A sequence encoding a calcium binding motif within the first TSP type-3 repeat of zebrafish Comp was targeted by CRISPR-Cas. The heterozygous and homozygous mutant Comp zebrafish displayed a patchy irregular Comp staining in 3 dpf myosepta, indicating a dominant phenotype. Electron microscopy revealed that the endoplasmic reticulum of myosepta fibroblasts is not affected in homozygous fish. The disorganized extracellular matrix may indicate that this mutation rather interferes with extracellular matrix assembly, similar to what is seen in a subgroup of chondrodysplasia patients. The early expression and easy detection of mutant Comp in zebrafish points to the potential of using the zebrafish model for large scale screening of small molecules that can improve secretion or function of disease-associated COMP mutants.
Tandem mass tag-based proteomic profiling revealed potential therapeutic targets and mechanisms of liraglutide for the treatment of impaired glucose tolerance
Authors: Guo Qiuyue , Han Cong , Xu Yunsheng , Chen Qingguang , Han Xu , Zhao Sen , Li Jie , Lu Hao
Published: November 2022

Objective

Based on the tandem mass tag (TMT) technique, our study investigated the potential therapeutic targets of Liraglutide (LIRA) on streptozotocin (STZ) induced impaired glucose tolerance (IGT) in rats and discuss the biological mechanism of the drug against IGT.

Methods

10 rats were randomly selected from 31 male wistar rats of specific pathogen free (SPF) grade as control group and fed with conventional chow, offered the remaining rats a high fat and high sugar (HFSD) diet combined with an intraperitoneal injection of STZ to establish the IGT model, and excluded 2 non-model rats. Specifically, the model rats were randomly divided into Model group (n=10) and LIRA group (n=9). In addition, the LIRA group was subcutaneously injected with 0.06 mg/kg LIRA, during which the metabolic parameters including body weight and fasting blood glucose were recorded. After 8 weeks, samples were taken under anesthesia. Then, the cell morphology was observed using HE staining, and immunofluorescence was performed on the pancreatic tissues of the three groups of rats. Besides, the expression of differential proteins in pancreatic tissues of the three groups of rats was determined by the TMT proteomic labeling. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological function analysis were performed on the intersection of Model and LIRA differential proteins.

Results

LIRA could not only significantly reduce blood glucose levels but also improve islet cell morphology and function in IGT rats. Among the differential proteins between the model group and the blank group, 44 were reversed after LIRA treatment, of which 14 were up-regulated, while 30 were down-regulated, including PPIF, MPRIP, CYP51, TXNL1, BCL-2, etc. (FC>1.1 or<0.909, P<0.05). According to the GO and KEGG analysis results, it was related to biological processes such as fatty acid metabolism and adipocyte generation, which involved multiple signaling pathways regulating the function of islet cells, such as MAPK, PI, Ras, FcγR, and unsaturated fatty acids, and pyruvate metabolism.

Conclusion

To sum up, LIRA participated in anti-IGT therapy through regulation of multiple target proteins and biological functions. This study is of great reference for further exploring the mechanism of action of LIRA at the protein level of IGT.
Cocultivation of Chinese prescription and intestine microbiota: SJZD alleviated the major symptoms of IBS-D subjects by tuning neurotransmitter metabolism
Authors: Xia Xiuwen , Xie Ya , Chen Qiaoqiao , Ding Dou , Wang Zongqin , Xu Yaji , Wang Yili , Wang Xiumin , Ding Weijun
Published: November 2022

Objective

Diarrhea-predominant irritable bowel syndrome (IBS-D) is a recurrent and common disease featuring dysbiotic intestinal microbiota, with limited treatments. Si-Jun-Zi Decoction (SJZD), a classic Chinese prescription, has been extensively used for IBS-D. This work aimed to explore the ex vivo interactions of SJZD and IBS-D’s intestinal microbiota.

Methods

Five samples of intestinal microbiota collected from IBS-D volunteers and five age-matched healthy controls were recruited from the Affiliated Hospital, Chengdu University of Traditional Chinese Medicine (TCM). A representative mixture of intestinal microbiota was composed of an equal proportion of these fecal samples. To simulate the clinical interaction, this microbiota was cocultivated with SJZD at clinical dosage in an anaerobic incubator at 37°C for 35 h. Microbiota and metabolic alterations were assessed by 16S rRNA gene sequencing in the V3/V4 regions and a nontargeted metabolome platform, respectively.

Results

After being cocultivated with SJZD, the dysbiotic intestine microbiota from IBS-D subjects was largely restored to those of the healthy controls. A total of 624 differentially expressed metabolites were detected by nontargeted metabolomics, of which 16 biomarkers were identified. These metabolites were then enriched into 11 pathways by KEGG, particularly those involved in neurotransmitter metabolism responses for the major symptom of IBS-D. Correlation analysis of bacterial metabolites demonstrated a synergistic pattern of neurotransmitter metabolism between Streptococcus and E. Shigella.

Conclusion

SJZD rescued the dysbiotic intestinal microbiota and ameliorated the dysfunctional neurotransmitter metabolism involved in IBS-D’s major symptoms.
Operation strategy and profitability analysis of independent energy storage participating in electricity market: A provincial case study in China
Authors: Gong Jiawei , Xiong Yun , Wu Hao , Chen Haoyong , Chen Jianrun , Xiao Dongliang
Published: November 2022

The new energy storage, referring to new types of electrical energy storage other than pumped storage, has excellent value in the power system and can provide corresponding bids in various types of electricity markets. As the scale of new energy storage continues to grow, China has issued several policies to encourage its application and participation in electricity markets. It is urgent to establish market mechanisms well adapted to energy storage participation and study the operation strategy and profitability of energy storage. Based on the development of the electricity market in a provincial region of China, this paper designs mechanisms for independent energy storage to participate in various markets. Then, its current and future operation strategies by division time or capacity for participation in each type of market are analyzed, and the profitability under various scenarios is calculated. Finally, based on the calculation results, the theoretical analysis basis for developing independent energy storage in the province and the policy formulation of participation in the market is provided.
Comparative study on numerical simulation based on CALPUFF and wind tunnel simulation of hazardous chemical leakage accidents
Authors: Zhang Rongqian , Li Mei , Ma Hancong
Published: November 2022

CALPUFF, as a Lagrangian puff modeling system, is mostly used in the field of atmospheric environment research and risk assessment. CALPUFF performs well for short-term and short-range release scenarios over complex terrain, as well as long-term and long-range transportation. Therefore, this article uses the CALPUFF model to simulate a toxic gas leakage accident in a hazardous chemical plant in an urban area, focusing on the influence of local buildings. Wind tunnel experiments are performed in accordance with the CALPUFF experiments to assess the model’s accuracy in cases of chemical leakage accidents. The results of the wind tunnel experiment are superimposed on the map of CALPUFF calculation, and the quantitative analysis is also performed. The comparative results show that the simulation results of the CALPUFF are mainly affected by factors such as wind direction, wind speed, and the complexity of the surface environment. With less influence of buildings, such as the south and north wind, the CALPUFF simulation is consistent with the wind tunnel experiment, having a correlation coefficient of over 0.7 in most cases, while under the east wind, the consistency is significantly lower due to the influence of buildings. In addition, it is found that the wind tunnel experiment is more accurate in the near field of the pollution source, while CALPUFF is more suitable for simulating the overall trend of gas dispersion. The comparison and evaluation of the capabilities of different methods on gas dispersion simulation are helpful in guiding the emergency response during hazardous chemical leakage accidents.
One size does not fit all: Variation in anatomical traits associated with emersion behavior in mudskippers (Gobiidae: Oxudercinae)
Authors: Corush Joel B. , Zhang Jie
Published: November 2022

Life histories involving transitions between differing habitats (i.e., aquatic to terrestrial or marine to freshwater) require numerous anatomical, physiological, and behavioral changes. Often, the traits associated with these changes are thought to come in suites, but all traits thought to be associated with particular life histories may not be required. While some traits are found in all species with a particular habitat transition, a grab bag approach may apply to other traits in that any trait may be sufficient for successful habitat transitions. We examine patterns of morphological traits associated with prolonged emersion in mudskipper, an amphibious fishes clade, where prolonged emersion appears twice. We test the evolutionary history of multiple characteristics associated with cutaneous respiration. We find most traits thought to be key for prolonged emersion show no phylogenetic signal and no tight correlation with prolonged emersion. Such traits appear in species with prolonged emersion but also non-emerging species. Only capillary density, which, when increased, allows for increased oxygen absorption, shows strong phylogenetic signal and correlation with prolonged emersion. Further experimental, functional genomics, and observational studies are needed to fully understand the mechanisms associated with each of these traits. With respect to traits associated with other particular behaviors, a comparative framework can be helpful in identifying evolutionary correlates.
Emerging roles of growth factors in osteonecrosis of the femoral head
Authors: Che Zhenjia , Song Yang , Zhu Liwei , Liu Tengyue , Li Xudong , Huang Lanfeng
Published: November 2022

Osteonecrosis of the femoral head (ONFH) is a potentially disabling orthopedic condition that requires total hip arthroplasty in most late-stage cases. However, mechanisms underlying the development of ONFH remain unknown, and the therapeutic strategies remain limited. Growth factors play a crucial role in different physiological processes, including cell proliferation, invasion, metabolism, apoptosis, and stem cell differentiation. Recent studies have reported that polymorphisms of growth factor-related genes are involved in the pathogenesis of ONFH. Tissue and genetic engineering are attractive strategies for treating early-stage ONFH. In this review, we summarized dysregulated growth factor-related genes and their role in the occurrence and development of ONFH. In addition, we discussed their potential clinical applications in tissue and genetic engineering for the treatment of ONFH.
Potential biomarkers: Identifying powerful tumor specific T cells in adoptive cellular therapy
Authors: Ge Wu , Dong Yuqian , Deng Yao , Chen Lujuan , Chen Juan , Liu Muqi , Wu Jianmin , Wang Wei , Ma Xiaoqian
Published: November 2022

Tumor-specific T cells (TSTs) are essential components for the success of personalized tumor-infiltrating lymphocyte (TIL)-based adoptive cellular therapy (ACT). Therefore, the selection of a common biomarker for screening TSTs in different tumor types, followed byex vivoexpansion to clinical number levels can generate the greatest therapeutic effect. However, studies on shared biomarkers for TSTs have not been realized yet. The present review summarizes the similarities and differences of a number of biomarkers for TSTs in several tumor types studied in the last 5 years, and the advantages of combining biomarkers. In addition, the review discusses the possible shortcomings of current biomarkers and highlights strategies to identify TSTs accurately using intercellular interactions. Finally, the development of TSTs in personalized TIL-based ACT for broader clinical applications is explored.
Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
Authors: Jardim-Santos Gabriela Profírio , Schulte Heidi Luise , Kurizky Patricia Shu , Gomes Ciro Martins , Nóbrega Otávio Tolêdo , de Gois Eliana Teles , de Carvalho Maíra Rocha Machado , Martins Francielle Pulccinelli , Nicola André Moraes , de Albuquerque Cleandro Pires , Espindola Laila Salmen , Naves Luciana Ansaneli , Soares Alexandre Anderson de Sousa Munhoz , Albuquerque Patrícia , Fontes Wagner , Amaral Laurence Rodrigues do , Gomes Matheus de Souza , Bertarini Pedro Luiz Lima , Brito-de-Sousa Joaquim Pedro , Campi-Azevedo Ana Carolina , Peruhype-Magalhães Vanessa , Teixeira-Carvalho Andrea , Valim Valéria , Martins-Filho Olindo Assis , da Mota Licia Maria Henrique
Published: November 2022

The present study applied distinct models of descriptive analysis to explore the integrative networks and the kinetic timeline of serum soluble mediators to select a set of systemic biomarkers applicable for the clinical management of COVID-19 patients. For this purpose, a total of 246 participants (82 COVID-19 and 164 healthy controls – HC) were enrolled in a prospective observational study. Serum soluble mediators were quantified by high-throughput microbeads array on hospital admission (D0) and at consecutive timepoints (D1-6 and D7-20). The results reinforce that the COVID-19 group exhibited a massive storm of serum soluble mediators. While increased levels of CCL3 and G-CSF were associated with the favorable prognosis of non-mechanical ventilation (nMV) or discharge, high levels of CXCL10 and IL-6 were observed in patients progressing to mechanical ventilation (MV) or death. At the time of admission, COVID-19 patients presented a complex and robust serum soluble mediator network, with a higher number of strong correlations involving IFN-γ, IL-1Ra and IL-9 observed in patients progressing to MV or death. Multivariate regression analysis demonstrates the ability of serum soluble mediators to cluster COVID-19 from HC. Ascendant fold change signatures and the kinetic timeline analysis further confirmed that the pairs “CCL3 and G-CSF” and “CXCL10 and IL-6” were associated with favorable or poor prognosis, respectively. A selected set of systemic mediators (IL-6, IFN-γ, IL-1Ra, IL-13, PDGF and IL-7) were identified as putative laboratory markers, applicable as complementary records for the clinical management of patients with severe COVID-19.
PD-L1 is upregulated in CD163+ tonsillar macrophages from children undergoing EBV primary infection
Authors: Moyano Agustina , Ferressini Natalia , De Matteo Elena , Preciado Maria Victoria , Chabay Paola
Published: November 2022

Epstein–Barr Virus (EBV) is a tumor associated virus that modulates not only the infected cells but also innate and adaptive immunity. Macrophages play a key role in tumor development and progression. Particularly, the M2 phenotype (CD163) with anti-inflammatory activity contributes to a favorable microenvironment for tumor development while the M1 (CD68) proinflammatory phenotype contributes to a restrictive one. In the context of pediatric EBV infection, little is known about macrophage contribution to PD-L1 expression, a molecule involved in immune exhaustion. We studied tonsils of primary infected (PI), healthy carriers (HC), reactivated (R), and not infected (NI) pediatric patients. Positive correlations were demonstrated for CD68+PD-L1+ in R and for CD163+PD-L1+ only in PI. Furthermore, CD163+PD-L1+ cell numbers were higher than PD-L1+CD68+ in PI patients. In addition, a positive correlation between PD-L1+CD163+ cells and LMP1 viral latent protein was observed in PI patients, and a positive correlation between PD-L1+CD68+ cells and BMRF1 lytic antigen was demonstrated. A positive correlation between TGF-β and PD-L1 expression was demonstrated in HC patients. Our findings indicate that EBV’s lytic and latent antigens might be regulating macrophages’ PD-L1 expression, particularly in PI patients, whereas, surprisingly, only TGF-β could be related to total PD-L1 upregulation. Given the relevance of macrophages and the PD-1/PD-L1 pathway in tumor progression and survival, more studies in early EBV infection could help to develop EBV-associated tumor therapies.
Kinship and genetic variation in aquarium-spawned Acropora hyacinthus corals
Authors: López-Nandam Elora H. , Payne Cheyenne Y. , Delbeek J. Charles , Dunker Freeland , Krol Lana , Larkin Lisa , Lev Kylie , Ross Richard , Schaeffer Ryan , Yong Steven , Albright Rebecca
Published: November 2022

Recent scientific advances in ex situ system design and operation make it possible to complete gametogenic cycles of broadcast spawning corals. Breeding corals in aquaria is a critical advance for population management, particularly genetic rescue and assisted gene flow efforts. Genetic rescue projects for corals are already underway to bring threatened species into ex situ culture and propagation, thereby preserving standing genetic variation. However, while breeding corals is increasingly feasible, the consequences of the aquarium environment on the genetic and phenotypic composition of coral populations is not yet known. The aquarium environment may in itself be a selective pressures on corals, but it also presents relaxed selective pressures in other respects. In 2019 and 2020, gravid Acropora hyacinthus coral colonies were collected from Palauan reefs and shipped to the California Academy of Sciences (CAS) in San Francisco. In both years, gametes were batch-fertilized to produce larvae that were then settled and reared to recruits. As of April 2021, when they were sampled for sequencing, 23 corals produced at CAS in 2019 and 16 corals produced at CAS in 2020 had survived for two years and one year, respectively. We sequenced the full genomes of the 39 offspring corals and their 15 potential parents to a median 26x depth of coverage. We find clear differential parentage, with some parents producing the vast majority of offspring, while the majority of parents produced no surviving offspring. After scanning 12.9 million single nucleotide polymorphisms (SNPs), we found 887 SNPs that may be under selection in the aquarium environment, and we identified the genes and pathways these SNPs may affect. We present recommendations for preserving standing genetic variation in aquarium-bred corals based on the results of this pilot project.
Small airways dysfunction in patients with systemic sclerosis and interstitial lung disease
Authors: Panagopoulos Panagiotis K. , Goules Andreas V. , Georgakopoulou Vasiliki E. , Kallianos Anastasios , Chatzinikita Eirini , Pezoulas Vasileios C. , Malagari Katerina , Fotiadis Dimitrios I. , Vlachoyiannopoulos Panayiotis , Vassilakopoulos Theodoros , Tzioufas Athanasios G.
Published: November 2022

Background

A number of studies report small airways involvement in patients with systemic sclerosis (SSc). Furthermore, small airways dysfunction is increasingly recognized in patients with interstitial lung disease (ILD) of idiopathic or autoimmune etiology. The objectives of this study were to evaluate small airways function in SSc patients with ILD and explore the effect of treatment on small airways function by using conventional and contemporary pulmonary function tests (PFTs).

Methods

This single-center, prospective, observational study included a total of 35 SSc patients, with and without ILD based on HRCT scan, evaluated by a special radiologist blindly. Clinical data were collected from all patients who were also assessed for HRCT findings of small airways disease. Small airways function was assessed by classic spirometry, measurement of diffusing capacity for carbon monoxide, body plethysmography, single breath nitrogen washout (N2SBW) and impulse oscillometry (IOS). The prevalence of small airways dysfunction according to R5–R20, phase III slopeN2SBW and CV/VC methodologies was calculated in the total SSc population. Pulmonary function tests were compared between: (a) SSc-ILD and non-ILD patients and (b) two time points (baseline and follow up visit) in a subset of SSc-ILD patients who received treatment for ILD and were re-evaluated at a follow up visit after 12 months.

Results

Phase III slopeN2SBW and R5–R20 showed the highest diagnostic performance for detecting small airways dysfunction among SSc patients (61 and 37.5%, respectively). Twenty three SSc patients were found with ILD and 14 of them had a 12-month follow up visit. SSc-ILD patients compared to those without ILD exhibited increased phase III slopeN2SBW ≥120% (p = 0.04), R5–R20 ≥0.07 kPa/L/s (p = 0.025), airway resistance (Raw) (p = 0.011), and special airway resistance (sRaw) (p = 0.02), and decreased specific airway conductance (sGaw) (p = 0.022), suggesting impaired small airways function in the SSc-ILD group. Radiographic features of SAD on HRCT were observed in 22% of SSc-ILD patients and in none of SSc-non-ILD patients. Comparison of PFTs between baseline and follow-up visit after 12 months in the 14 SSc-ILD treated patients, showed improvement of phase III slopeN2SBW (p = 0.034), R5–R20 (p = 0.035) and Raw (p = 0.044) but not sRaw and sGaw parameters.

Conclusion

Phase III slopeN2SBW and R5–R20 may reveal small airways dysfunction in SSc associated ILD before structural damage and may be partially improved in a subset of patients receiving treatment for ILD.
Study on the mechanisms of action of berberine combined with fluconazole against fluconazole-resistant strains of Talaromyces marneffei
Authors: Kai-su Pan , Hong Luo , Dong-yan Zheng , Yan-qing Zheng , Andrianopoulos Alex , Latgé Jean-Paul , Cun-wei Cao
Published: November 2022

Talaromyces (Penicillium) marneffei (T. marneffei) is a thermally dimorphic fungus that can cause opportunistic systemic mycoses. Our previous study demonstrated that concomitant use of berberine (BBR) and fluconazole (FLC) showed a synergistic action against FLC-resistant T. marneffei (B4) in vitro. In this paper, we tried to figure out the antifungal mechanisms of BBR and FLC in T. marneffei FLC-resistant. In the microdilution test, the minimum inhibitory concentration (MIC) of FLC was 256 μg/ml before FLC and BBR combination, and was 8 μg/ml after combination, the partial inhibitory concentration index (FICI) of B4 was 0.28. After the treatments of BBR and FLC, the studies revealed that (i) increase reactive oxygen species (ROS), (ii) reduce ergosterol content, (iii) destroy the integrity of cell wall and membrane, (iv) decrease the expression of genes AtrF, MDR1, PMFCZ, and Cyp51B however ABC1 and MFS change are not obvious. These results confirmed that BBR has antifungal effect on T. marneffei, and the combination with FLC can restore the susceptibility of FLC-resistant strains to FLC, and the reduction of ergosterol content and the down-regulation of gene expression of AtrF, Mdr1, PMFCZ, and Cyp51B are the mechanisms of the antifungal effect after the combination, which provides a theoretical basis for the application of BBR in the treatment of Talaromycosis and opens up new ideas for treatment of Talaromycosis.