bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing Carbon Sequestration in Forest Management Plans Using Advanced Algorithms: A Case Study of Greater Khingan Mountains
Authors: Zhang Weitian , Shao Hanqin , Sun Haitao , Zhang Wei , Yan Qinglun
Published: September 2023

The Paris Agreement aims to combat climate change by reducing greenhouse gas emissions, with bioenergy identified as a potential solution. However, concerns remain about its impact on carbon stocks and the optimal timing for implementation. To address these challenges, we propose a comprehensive multi-objective optimization model for forest management that maximizes carbon sequestration and economic benefits. Our model integrates three key components: (1) a sophisticated carbon-sequestration model encompassing living plants, wood forest products, and soil and microbial carbon uptake, (2) dynamic factors such as forest fires and extreme weather events, and (3) an economic benefits model focused on wood-processing products. We optimized the forest-management strategy over ten years by leveraging the simulated annealing and Karush–Kuhn–Tucker (KKT) algorithms. Through simulations using data from China’s Greater Khingan Mountains region, we explored the optimal logging plans for maximizing carbon sequestration without external factors. Our results revealed that the optimized logging plans significantly enhance carbon sequestration compared to proportionally averaged logging plans. Next, we investigated the impact of external factors on forest management, specifically wildfires and extreme weather events. Our findings demonstrate that wildfires have a more-substantial detrimental effect on the absolute value of carbon sequestration and the extent of improvement achieved through model optimization. At the same time, extreme cold primarily affects the growth rate of carbon sequestration. We employed a linear-weighting approach and the Analytic Hierarchy Process (AHP) to address the trade-offs between carbon sequestration and economic benefits to transform the multi-objective optimization function into a single objective. The results showed that the optimized harvesting schedule can lead to improved economic benefits compared to uniformly harvesting trees. Moreover, the joint optimization approach enabled us to identify optimal solutions that balance carbon sequestration and economic benefits, offering sustainable forest management strategies. Our study provides valuable quantitative insights into forest management strategies that balance carbon sequestration and economic benefits, making it highly relevant for real-world applications.
Relationships between Climate Variability and Radial Growth of Larix potaninii at the Upper Altitudinal Limit in Central Hengduan Mountain, Southwestern China
Authors: Yue Haitao , Li Jianing , Xie Siyu , Chen Hai , Tian Kun , Sun Mei , Zhang Dacai , Zhang Yun
Published: September 2023

Improved understanding of the responses of stem radial growth to climates is necessary for modeling and predicting the response of forest ecosystems to future climate change. We used dendrochronological methods to study climate effects on the radial growth of a subalpine deciduous conifer, Larix potaninii. Tree-ring residual chronologies were developed for five sites at the upper distributional limits in the Central Hengduan Mountains, Southwestern China. Redundancy analysis and response function were used to compare inter-annual variability in growth sensitivity among the chronologies and to identity key climatic factors controlling tree radial growth. The results showed that both precipitation and temperature influenced tree growth, and response patterns were consistent for five chronologies. During the current year’s early growing season (Tmean in May and Tmax in June), temperature positively affected the radial growth of L. potaninii, while September Tmin and October precipitation in the previous year and May and June precipitation in the current year all had negative impacts on its radial growth. L. potaninii growth appeared to be mainly limited by photothermal conditions in May and June. In the context of increasing CO2 concentrations accompanied with warmer temperatures, future climate change would likely stimulate the radial growth of L. potaninii in Central Hengduan Mountain.
Phenotypic Characterisation and Molecular Identification of Potentially Probiotic Lactobacillus sp. Isolated from Fermented Rice
Authors: Jeyagowri Nimalan , Ranadheera Chaminda Senaka , Manap Mohd Yazid , Gamage Ashoka , Merah Othmane , Madhujith Terrence
Published: September 2023

Fermented rice is known as a healthy food due to the presence of lactic acid bacteria. The study was carried out to identify and characterise the lactic acid bacteria (LAB) from white and red fermented rice Bg (Bathalagoda) varieties. Fermentation was carried out naturally by soaking red, white, raw, and cooked rice in sterile distilled water (1:3) overnight at 27 °C in an earthen pot. Potentially probiotic bacterial were isolated and the species of the isolated lactic acid bacteria were confirmed based on 16S rDNA gene sequencing and were studied for phenotypic characteristics, including morphological, physiological (growth temperature, salt tolerance, milk coagulation), and biochemical (carbohydrate fermentation pattern) characteristics, using API 50CH kits. Distinct clusters of cocci (48), diplococci (30), and rod-shaped bacteria (30) were observed in fermented rice. Five species of lactic acid bacteria were identified, including Latilactobacillus curvatus GRLb1, 2, 10, and 11 (the predominant Bacillus species); Latilactobacillus graminis GRLb 8; Limosilactobacillus fermentum GRLb17; Weissella confuse GRLb4; and Pediococcus pentosaceus GRLc1. The base pair length of amplified DNA for the isolates was 1500 Bp. Most of the isolates were able to grow at temperatures ranging from 10 °C to 45 °C, tolerate up to 6.5% salt, and coagulate milk with homofermentative characteristics. The beneficial physiological and biochemical properties of isolated Lactobacillus species from fermented rice revealed their potential applications in the food industry. The similar species of bacteria that were isolated from different sources show their probiotic characteristics. Further studies are recommended to confirm their probiotic properties and health benefits.
FREDY: Federated Resilience Enhanced with Differential Privacy
Authors: Anastasakis Zacharias , Velivassaki Terpsichori-Helen , Voulkidis Artemis , Bourou Stavroula , Psychogyios Konstantinos , Skias Dimitrios , Zahariadis Theodore
Published: September 2023

Federated Learning is identified as a reliable technique for distributed training of ML models. Specifically, a set of dispersed nodes may collaborate through a federation in producing a jointly trained ML model without disclosing their data to each other. Each node performs local model training and then shares its trained model weights with a server node, usually called Aggregator in federated learning, as it aggregates the trained weights and then sends them back to its clients for another round of local training. Despite the data protection and security that FL provides to each client, there are still well-studied attacks such as membership inference attacks that can detect potential vulnerabilities of the FL system and thus expose sensitive data. In this paper, in order to prevent this kind of attack and address private data leakage, we introduce FREDY, a differential private federated learning framework that enables knowledge transfer from private data. Particularly, our approach has a teachers–student scheme. Each teacher model is trained on sensitive, disjoint data in a federated manner, and the student model is trained on the most voted predictions of the teachers on public unlabeled data which are noisy aggregated in order to guarantee the privacy of each teacher’s sensitive data. Only the student model is publicly accessible as the teacher models contain sensitive information. We show that our proposed approach guarantees the privacy of sensitive data against model inference attacks while it combines the federated learning settings for the model training procedures.
Further Study and Development of Correlations for Heat Transfer during Subcooled Boiling in Plain Channels
Authors: Shah Mirza M.
Published: September 2023

The author’s published correlations for subcooled boiling in channels are further studied and developed in this work. The areas explored include choice of equivalent diameters for annuli and partially heated channels, effects of flow direction, micro-gravity, and orientation of heated surface. A new correlation is developed, which is a modification of the author’s earlier correlation. The author’s previous correlations and the new correlation are compared with a very wide range of test data for round tubes, rectangular channels, and annuli. Several other correlations are also compared with the same data. The authors’ correlations provide good agreement with data, the new correlation giving the least deviation. The data included hydraulic diameters from 0.176 to 22.8 mm, reduced pressure from 0.0046 to 0.922, subcooling from 0 to 165 K, mass flux from 59 to 31,500 kgm−2s−1, all flow directions, and terrestial to micro gravity. The new correlation has mean absolute deviation (MAD) of 13.3% with 2270 data points from 49 sources. Correlations by others had MAD of 18% to 116%. The results are presented and discussed.
Utilization of Algae Extracts as Natural Antibacterial and Antioxidants for Controlling Foodborne Bacteria in Meat Products
Authors: Hamad Gamal M. , Samy Haneen , Mehany Taha , Korma Sameh A. , Eskander Michael , Tawfik Rasha G. , EL-Rokh Gamal E. A. , Mansour Alaa M. , Saleh Samaa M. , EL Sharkawy Amany , Abdelfttah Hesham E. A. , Khalifa Eman
Published: September 2023

Padina pavonica, Hormophysa cuneiformis, and Corallina officinalis are three types of algae that are assumed to be used as antibacterial agents. Our study’s goal was to look into algal extracts’ potential to be used as food preservative agents and to evaluate their ability to inhibit pathogenic bacteria in several meat products (pastirma, beef burger, luncheon, minced meat, and kofta) from the local markets in Alexandria, Egypt. By testing their antibacterial activity, results demonstrated that Padina pavonica showed the highest antibacterial activity towards Bacillus cereus, Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, Salmonella spp., and Klebsiella pneumoniae. Padina pavonica extract also possesses most phenolic and flavonoid content overall. It has 24 mg gallic acid equivalent/g and 7.04 mg catechol equivalent/g, respectively. Moreover, the algae extracts were tested for their antioxidant activity, and the findings were measured using ascorbic acid as a benchmark. The IC50 of ascorbic acid was found to be 25.09 μg/mL, while Padina pavonica exhibited an IC50 value of 267.49 μg/mL, Corallina officinalis 305.01 μg/mL, and Hormophysa cuneiformis 325.23 μg/mL. In this study, Padina pavonica extract was utilized in three different concentrations (Treatment 1 g/100 g, Treatment 2 g/100 g, and Treatment 3 g/100 g) on beef burger as a model. The results showed that as the concentration of the extract increased, the bacterial inhibition increased over time. Bacillus cereus was found to be the most susceptible to the extract, while Streptococcus pyogenes was the least. In addition, Padina pavonica was confirmed to be a safe compound through cytotoxicity testing. After conducting a sensory evaluation test, it was confirmed that Padina pavonica in meat products proved to be a satisfactory product.
Valorisation of Side Stream Products through Green Approaches: The Rapeseed Meal Case
Authors: Cairone Francesco , Allevi Dario , Cesa Stefania , Fabrizi Giancarlo , Goggiamani Antonella , Masci Domiziana , Iazzetti Antonia
Published: September 2023

Rapeseed meal (RSM) is a by-product of rapeseed oil extraction and is a rich source of bioactive compounds, including proteins and antioxidants. This study compared two methods for extracting antioxidants from RSM: conventional ethanol Soxhlet extraction and supercritical CO2 extraction. These procedures were applied to both native RSM and RSM after protein removal to evaluate their bio-compound composition and potential applications. HPLC-DAD, NMR, and GC/MS analyses revealed a rich polyphenolic profile in the extracts, including the presence of sinapic acid. The concentration of sinapic acid varied depending on the extraction method used. The anti-radical activity of the extracts was also analysed using the DPPH assay, which confirmed the potential of RSM as a source of antioxidants for use in cosmetics, food, and pharmaceutical formulations.
Influence of Lifestyle and Dietary Habits on the Prevalence of Food Allergies: A Scoping Review
Authors: Rennie Gardiner Henric , Zhao Jinlong , Camus-Ela Mukeshimana , Shi Jialu , Jiang Lan , Zhang Lili , Wang Jin , Raghavan Vijaya
Published: September 2023

Changes in behavior, lifestyle, and nutritional patterns have influenced many potential risk variables globally. In recent decades, food allergies (FAs) have been elevated to a severe public health issue both in developed countries and developing countries (third-world countries). This study aims to evaluate the effects caused by certain factors such as lifestyle and dietary habits on food allergies, review the association of lifestyle and dietary habit status with FAs, and outline why more people are allergic to food sources as a result of lifestyle changes and dietary habits. We searched electronic international databases including Scopus, PubMed, Google Scholar, and Web of Science using combinations of keywords. Utilizing Excel, the relevant studies were included and the irrelevant studies were excluded, and Mendeley was used for referencing and also to remove duplicates. The framework proposed by Arksey and O’Malley was used for this scoping review. The papers published in the databases from 2016 to 2020 were extracted. A total of eight studies were extracted, and this scoping review was carried out according to the risk factors. In our review, we found that some lifestyle choices (Caesarean section and antibiotics) and dietary habits (n-3 PUFA, fast food, duration of dietary intervention, and vitamin D), were important contributing factors for FA.
Design of Pectin-Based Hydrogel Microspheres for Targeted Pulmonary Delivery
Authors: Chai Andy , Schmidt Keagan , Brewster Gregory , Xiong Lu Shi Peng , Church Benjamin , Wahl Timothy , Sadabadi Hamed , Kumpaty Subha , Zhang Wujie
Published: September 2023

Pulmonary drug delivery via microspheres has gained growing interest as a noninvasive method for therapy. However, drug delivery through the lungs via inhalation faces great challenges due to the natural defense mechanisms of the respiratory tract, such as the removal or deactivation of drugs. This study aims to develop a natural polymer-based microsphere system with a diameter of around 3 μm for encapsulating pulmonary drugs and facilitating their delivery to the deep lungs. Pectin was chosen as the foundational material due to its biocompatibility and degradability in physiological environments. Electrospray was used to produce the pectin-based hydrogel microspheres, and Design-Expert software was used to optimize the production process for microsphere size and uniformity. The optimized conditions were determined to be as follows: pectin/PEO ratio of 3:1, voltage of 14.4 kV, distance of 18.2 cm, and flow rate of 0.95 mL/h. The stability and responsiveness of the pectin-based hydrogel microspheres can be altered through coatings such as gelatin. Furthermore, the potential of the microspheres for pulmonary drug delivery (i.e., their responsiveness to the deep lung environment) was investigated. Successfully coated microspheres with 0.75% gelatin in 0.3 M mannitol exhibited improved stability while retaining high responsiveness in the simulated lung fluid (Gamble’s solution). A gelatin-coated pectin-based microsphere system was developed, which could potentially be used for targeted drug delivery to reach the deep lungs and rapid release of the drug.
Vedolizumab as Rescue Therapy in Carboplatin-Gemcitabine-Induced Triggered Acute Severe Ulcerative Colitis Flare-Up
Authors: Pellegrino Raffaele , Fasano Morena , Morgillo Floriana , Palladino Giovanna , Vassallo Isabella , Pirozzi Mario , Imperio Giuseppe , Auletta Salvatore , Ventura Andrea , Panarese Iacopo , Federico Alessandro , Gravina Antonietta Gerarda
Published: September 2023

Approximately 20% of patients with ulcerative colitis (UC) develop acute severe UC (ASUC), for which intravenous systemic steroid therapy and possibly infliximab-based rescue therapy are generally imposed. However, there are no significant guideline recommendations on ASUC regarding vedolizumab as an alternative in this setting. A case report was presented where a patient with steroid-dependent UC developed ASUC induced by second-line chemotherapy. Treatment with intravenous methylprednisolone was imposed, but there was no reduction in bowel movements in the days following admission. Rescue therapy with infliximab was contraindicated because of the oncologic history. Surgical consultation, contraindicated colectomy, and administration of vedolizumab 300 mg were initiated. After infusion with vedolizumab, there was a significant reduction in bowel movements starting the day after infusion until normalisation of bowel movements within three days and the concomitant normalisation of inflammatory indices. The patient is currently in clinical remission, on therapy with vedolizumab 108 mg subcutaneously every two weeks, and is in oncologic follow-up for pulmonary neoplasm. This case highlights the novel potential of vedolizumab as a possible rescue therapy in ASUC, especially in special populations, where it may offer a better safety profile. Although cyclosporine and infliximab still represent the mainstays of salvage therapy for steroid-refractory ASUC, new therapeutic agents may also be effective, such as vedolizumab, ustekinumab, and anti-Janus kinase agents.
Sodium Silicate Improves Cucumber Seedling Growth and Substrate Nutrients and Reduces Heavy Metal Accumulation in Plants
Authors: Tian Wei , Li Zhaoxuan , Gong Kaixuan , Wang Xiaodong , Shah Sadiq , Wang Xiaozhuo , Zhang Xueyan
Published: September 2023

The gasification filter cake (GFC) has great application potential for improving the characteristics of seedling substrates due to its nutrient richness and excellent water retention capacity. However, GFCs leach heavy metals easily and thus pose certain ecological risks. Sodium silicate can enhance plant resistance to heavy metal toxicity by fixing heavy metals. This study investigated the impact of sodium silicate on cucumber plant growth, the chemical characterization of the substrate, and the distribution and transfer of heavy metals. Sodium silicate was added to the seedling substrate mix at mass rates of 0 g/kg−1 (GFC0), 2 g/kg−1 (GFC2), 4 g/kg−1 (GFC4), and 8 g/kg−1 (GFC8). The seedling substrate was composed of a commercial matrix, caragana compost, and GFC (m:m 7:7:2). The GFC increased the content of total phosphorus (P), available phosphorus (P), and available potassium (K) in the substrate by 31.58%, 16.58%, and 80.10%, respectively. Conversely, the GFC decreased the plant height by 12.3%. Adding sodium silicate to the GFC increased the chlorophyll content of the plants, fixed heavy metals in the substrate, and promoted nutrient absorption and utilization by the plants. Compared with GFC0 without sodium silicate, adding sodium silicate at a mass rate of 2 g/kg−1 (GFC2) reduced the chromium, lead, and cadmium contents by 51.13%, 26.37%, and 90.04%, respectively, which effectively alleviated heavy metal stress and was more conducive to plant growth.
Teacher and Caregiver Perspectives on Water Is K’é: An Early Child Education Program to Promote Healthy Beverages among Navajo Children
Authors: Kahn Carmella B. , John Brianna , Shin Sonya S. , Whitman Rachel , Yazzie Asia Soleil , Goldtooth-Halwood Renee , Hecht Ken , Hecht Christina , Vollmer Laura , Egge Malyssa , Nelson Nora , Bitah Kerlissa , George Carmen
Published: September 2023

The Water is K’é program was developed to increase water consumption and decrease consumption of sugar-sweetened beverages for young children and caregivers. The pilot program was successfully delivered by three Family and Child Education (FACE) programs on the Navajo Nation using a culturally centered curriculum between 2020 to 2022. The purpose of this research was to understand teacher and caregiver perspectives of program feasibility, acceptability, impact, and other factors influencing beverage behaviors due to the pilot program. Nine caregivers and teachers were interviewed between June 2022 and December 2022, and a study team of four, including three who self-identified as Navajo, analyzed the data using inductive thematic analysis and consensus building to agree on codes. Five themes emerged, including feasibility, acceptability, impact, suggestions for future use of the program, and external factors that influenced water consumption. The analysis showed stakeholders’ strong approval for continuing the program based on impact and acceptability, and identified factors that promote the program and barriers that can be addressed to make the program sustainable. Overall, the Water is K’é program and staff overcame many challenges during the COVID-19 pandemic to support healthy behavior change that had a rippled influence among children, caregivers, teachers, and many others.
Chronic Occupational Exposure to Traffic Pollution Is Associated with Increased Carotid Intima-Media Thickness in Healthy Urban Traffic Control Police
Authors: Balogun Abdulrazak O. , Weigel M. Margaret , Estévez Edmundo , Armijos Rodrigo X.
Published: September 2023

Urban traffic officers in many low- and middle-income countries are exposed to high levels of traffic-related air pollutants (TRAP) while working vehicle control on heavily congested streets. The impact of chronic TRAP exposure on the cardiovascular health, including the carotid intima-media thickness (CIMT), of this outdoor occupational group remains unclear. This cross-sectional study compared the average mean and maximum CIMT measurements of two groups of relatively young, healthy traffic police (32 ± 7 years; 77% male) in Quito, Ecuador, who were without clinical evidence of serious cardiovascular or other disease. Previously published background data on PM10 (a TRAP surrogate) indicated that street levels of the pollutant were several orders of magnitude higher at the street intersections worked by traffic police compared to those working only in an office. Accordingly, officers permanently assigned to daily traffic control duties requiring them to stand 0–3 m from heavily trafficked street intersections were assigned to the high exposure group (n = 61). The control group (n = 54) consisted of officers from the same organization who were permanently assigned to office duties inside an administration building. Mean and maximum CIMT were measured with ultrasound. General linear models were used to compare the CIMT measurements of the high exposure and control groups, adjusting for covariates. The adjusted average mean and maximum CIMT measures of the high exposure group were increased by 11.5% and 10.3%, respectively, compared to the control group (p = 0.0001). These findings suggest that chronic occupational exposure to TRAP is associated with increased CIMT in traffic police. This is important since even small increases in arterial thickening over time may promote earlier progression to clinical disease and increased premature mortality risk.
Extracellular Vesicles in Liver Transplantation: Current Evidence and Future Challenges
Authors: De Stefano Nicola , Calleri Alberto , Faini Angelo Corso , Navarro-Tableros Victor , Martini Silvia , Deaglio Silvia , Patrono Damiano , Romagnoli Renato
Published: September 2023

Extracellular vesicles (EVs) are emerging as a promising field of research in liver disease. EVs are small, membrane-bound vesicles that contain various bioactive molecules, such as proteins, lipids, and nucleic acids and are involved in intercellular communication. They have been implicated in numerous physiological and pathological processes, including immune modulation and tissue repair, which make their use appealing in liver transplantation (LT). This review summarizes the current state of knowledge regarding the role of EVs in LT, including their potential use as biomarkers and therapeutic agents and their role in graft rejection. By providing a comprehensive insight into this emerging topic, this research lays the groundwork for the potential application of EVs in LT.
Identification of a Non-Invasive Urinary Exosomal Biomarker for Diabetic Nephropathy Using Data-Independent Acquisition Proteomics
Authors: Ding Xiaonan , Zhang Dong , Ren Qinqin , Hu Yilan , Wang Jifeng , Hao Jing , Wang Haoran , Zhao Xiaolin , Wang Xiaochen , Song Chenwen , Du Junxia , Yang Fuquan , Zhu Hanyu
Published: September 2023

Diabetic nephropathy (DN), as the one of most common complications of diabetes, is generally diagnosed based on a longstanding duration, albuminuria, and decreased kidney function. Some patients with the comorbidities of diabetes and other primary renal diseases have similar clinical features to DN, which is defined as non-diabetic renal disease (NDRD). It is necessary to distinguish between DN and NDRD, considering they differ in their pathological characteristics, treatment regimes, and prognosis. Renal biopsy provides a gold standard; however, it is difficult for this to be conducted in all patients. Therefore, it is necessary to discover non-invasive biomarkers that can distinguish between DN and NDRD. In this research, the urinary exosomes were isolated from the midstream morning urine based on ultracentrifugation combined with 0.22 μm membrane filtration. Data-independent acquisition-based quantitative proteomics were used to define the proteome profile of urinary exosomes from DN (n = 12) and NDRD (n = 15) patients diagnosed with renal biopsy and Type 2 diabetes mellitus (T2DM) patients without renal damage (n = 9), as well as healthy people (n = 12). In each sample, 3372 ± 722.1 proteins were identified on average. We isolated 371 urinary exosome proteins that were significantly and differentially expressed between DN and NDRD patients, and bioinformatic analysis revealed them to be mainly enriched in the immune and metabolic pathways. The use of least absolute shrinkage and selection operator (LASSO) logistic regression further identified phytanoyl-CoA dioxygenase domain containing 1 (PHYHD1) as the differential diagnostic biomarker, the efficacy of which was verified with another cohort including eight DN patients, five NDRD patients, seven T2DM patients, and nine healthy people. Additionally, a concentration above 1.203 μg/L was established for DN based on the ELISA method. Furthermore, of the 19 significantly different expressed urinary exosome proteins selected by using the protein–protein interaction network and LASSO logistic regression, 13 of them were significantly related to clinical indicators that could reflect the level of renal function and hyperglycemic management.
Metabolomic Analysis of Pediatric Patients with Idiosyncratic Drug-Induced Liver Injury According to the Updated RUCAM
Authors: Andújar-Vera Francisco , Alés-Palmer María Luisa , Muñoz-de-Rueda Paloma , Iglesias-Baena Iván , Ocete-Hita Esther
Published: September 2023

Hepatotoxicity, a common adverse drug effect, has been extensively studied in adult patients. However, it is equally important to investigate this condition in pediatric patients to develop personalized treatment strategies for children. This study aimed to identify plasma biomarkers that characterize hepatotoxicity in pediatric patients through an observational case–control study. Metabolomic analysis was conducted on 55 pediatric patients with xenobiotic liver toxicity and 88 healthy controls. The results revealed clear differences between the two groups. Several metabolites, including hydroxydecanoylcarnitine, octanoylcarnitine, lysophosphatidylcholine, glycocholic acid, and taurocholic acid, were identified as potential biomarkers (area under the curve: 0.817; 95% confidence interval: 0.696–0.913). Pathway analysis indicated involvement of primary bile acid biosynthesis and the metabolism of taurine and hypotaurine (p < 0.05). The findings from untargeted metabolomic analysis demonstrated an increase in bile acids in children with hepatotoxicity. The accumulation of cytotoxic bile acids should be further investigated to elucidate the role of these metabolites in drug-induced liver injury.
Identification of an NF1 Microdeletion with Optical Genome Mapping
Authors: Büki Gergely , Bekő Anna , Bödör Csaba , Urbán Péter , Németh Krisztina , Hadzsiev Kinga , Fekete György , Kehrer-Sawatzki Hildegard , Bene Judit
Published: September 2023

Neurofibromatosis type 1 (NF1) is a clinically heterogeneous neurocutaneous disorder inherited in autosomal dominant manner. Approximately 5–10% of the cases are caused by NF1 microdeletions involving the NF1 gene and its flanking regions. Microdeletions, which lead to more severe clinical manifestations, can be subclassified into four different types (type 1, 2, 3 and atypical) according to their size, the genomic location of the breakpoints and the number of genes included within the deletion. Besides the prominent hallmarks of NF1, patients with NF1 microdeletions frequently exhibit specific additional clinical manifestations like dysmorphic facial features, macrocephaly, overgrowth, global developmental delay, cognitive disability and an increased risk of malignancies. It is important to identify the genes co-deleted with NF1, because they are likely to have an effect on the clinical manifestation. Multiplex ligation-dependent probe amplification (MLPA) and microarray analysis are the primary techniques for the investigation of NF1 microdeletions. However, based on previous research, optical genome mapping (OGM) could also serve as an alternative method to identify copy number variations (CNVs). Here, we present a case with NF1 microdeletion identified by means of OGM and demonstrate that this novel technology is a suitable tool for the identification and classification of the NF1 microdeletions.
The Role of Neutrophil Extracellular Traps (NETs) in the Pathogenesis of Systemic Lupus Erythematosus and Antiphospholipid Syndrome
Authors: Reshetnyak Tatiana , Nurbaeva Kamila
Published: September 2023

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease of unknown aetiology [...]
A Comparison of PKD2L1-Expressing Cerebrospinal Fluid Contacting Neurons in Spinal Cords of Rodents, Carnivores, and Primates
Authors: Liu Xiaohe , Rich Karen , Nasseri Sohail M. , Li Guifa , Hjæresen Simone , Finsen Bente , Scherberger Hansjörg , Svenningsen Åsa , Zhang Mengliang
Published: September 2023

Cerebrospinal fluid contacting neurons (CSF-cNs) are a specific type of neurons located around the ventricles in the brain and the central canal in the spinal cord and have been demonstrated to be intrinsic sensory neurons in the central nervous system. One of the important channels responsible for the sensory function is the polycystic kidney disease 2-like 1 (PKD2L1) channel. Most of the studies concerning the distribution and function of the PKD2L1-expressing CSF-cNs in the spinal cord have previously been performed in non-mammalian vertebrates. In the present study immunohistochemistry was performed to determine the distribution of PKD2L1-immunoreactive (IR) CSF-cNs in the spinal cords of four mammalian species: mouse, rat, cat, and macaque monkey. Here, we found that PKD2L1-expressing CSF-cNs were present at all levels of the spinal cord in these animal species. Although the distribution pattern was similar across these species, differences existed. Mice and rats presented a clear PKD2L1-IR cell body labeling, whereas in cats and macaques the PKD2L1-IR cell bodies were more weakly labeled. Ectopic PKD2L1-IR neurons away from the ependymal layer were observed in all the animal species although the abundance and the detailed locations varied. The apical dendritic protrusions with ciliated fibers were clearly seen in the lumen of the central canal in all the animal species, but the sizes of protrusion bulbs were different among the species. PKD2L1-IR cell bodies/dendrites were co-expressed with doublecortin, MAP2 (microtubule-associated protein 2), and aromatic L-amino acid decarboxylase, but not with NeuN (neuronal nuclear protein), indicating their immature properties and ability to synthesize monoamine transmitters. In addition, in situ hybridization performed in rats revealed PKD2L1 mRNA expression in the cells around the central canal. Our results indicate that the intrinsic sensory neurons are conserved across non-mammalian and mammalian vertebrates. The similar morphology of the dendritic bulbs with ciliated fibers (probably representing stereocilia and kinocilia) protruding into the central canal across different animal species supports the notion that PKD2L1 is a chemo- and mechanical sensory channel that responds to mechanical stimulations and maintains homeostasis of the spinal cord. However, the differences of PKD2L1 distribution and expression between the species suggest that PKD2L1-expressing neurons may receive and process sensory signals differently in different animal species.
Social Media Analytics on Russia–Ukraine Cyber War with Natural Language Processing: Perspectives and Challenges
Authors: Sufi Fahim
Published: September 2023

Utilizing social media data is imperative in comprehending critical insights on the Russia–Ukraine cyber conflict due to their unparalleled capacity to provide real-time information dissemination, thereby enabling the timely tracking and analysis of cyber incidents. The vast array of user-generated content on these platforms, ranging from eyewitness accounts to multimedia evidence, serves as invaluable resources for corroborating and contextualizing cyber attacks, facilitating the attribution of malicious actors. Furthermore, social media data afford unique access to public sentiment, the propagation of propaganda, and emerging narratives, offering profound insights into the effectiveness of information operations and shaping counter-messaging strategies. However, there have been hardly any studies reported on the Russia–Ukraine cyber war harnessing social media analytics. This paper presents a comprehensive analysis of the crucial role of social-media-based cyber intelligence in understanding Russia’s cyber threats during the ongoing Russo–Ukrainian conflict. This paper introduces an innovative multidimensional cyber intelligence framework and utilizes Twitter data to generate cyber intelligence reports. By leveraging advanced monitoring tools and NLP algorithms, like language detection, translation, sentiment analysis, term frequency–inverse document frequency (TF-IDF), latent Dirichlet allocation (LDA), Porter stemming, n-grams, and others, this study automatically generated cyber intelligence for Russia and Ukraine. Using 37,386 tweets originating from 30,706 users in 54 languages from 13 October 2022 to 6 April 2023, this paper reported the first detailed multilingual analysis on the Russia–Ukraine cyber crisis in four cyber dimensions (geopolitical and socioeconomic; targeted victim; psychological and societal; and national priority and concerns). It also highlights challenges faced in harnessing reliable social-media-based cyber intelligence.
Prosthesis–Patient Mismatch and Aortic Root Enlargement: Indications, Techniques and Outcomes
Authors: Fazmin Ibrahim Talal , Ali Jason M.
Published: September 2023

Prosthesis–patient mismatch (PPM) is defined as implanting a prosthetic that is insufficiently sized for the patient receiving it. PPM leads to high residual transvalvular gradients post-aortic valve replacement and consequently results in left ventricular dysfunction, morbidity and mortality in both the short and long term. Younger patients and patients with poor preoperative left ventricular function are more vulnerable to increased mortality secondary to PPM. There is debate over the measurement of valvular effective orifice area (EOA) and variation exists in how manufacturers report the EOA. The most reliable technique is using in vivo echocardiographic measurements to create tables of predicted EOAs for different valve sizes. PPM can be prevented surgically in patients at risk through aortic root enlargement (ARE). Established techniques include the posterior enlargement through Nicks and Manouguian procedures, and aortico-ventriculoplasty with the Konno–Rastan procedure, which allows for a greater enlargement but carries increased surgical risk. A contemporary development is the Yang procedure, which uses a Y-shaped incision created through the non- and left-coronary cusp commissure, undermining the nadirs of the non- and left-coronary cusps. Early results are promising and demonstrate an ability to safely increase the aortic root by up to two to three sizes. Aortic root enlargement thus remains a valuable and safe tool in addressing PPM, and should be considered during surgical planning.
Biomarkers of Collagen Metabolism Are Associated with Left Ventricular Function and Prognosis in Dilated Cardiomyopathy: A Multi-Modal Study
Authors: Raafs Anne , Adriaans Bouke , Henkens Michiel , Verdonschot Job , Abdul Hamid Myrurgia , Díez Javier , Knackstedt Christian , van Empel Vanessa , Brunner-La Rocca Hans-Peter , González Arantxa , Wildberger Joachim , Heymans Stephane , Hazebroek Mark
Published: September 2023

Background: Collagen cross-linking is a fundamental process in dilated cardiomyopathy (DCM) and occurs when collagen deposition exceeds degradation, leading to impaired prognosis. This study investigated the associations of collagen-metabolism biomarkers with left ventricular function and prognosis in DCM. Methods: DCM patients who underwent endomyocardial biopsy, blood sampling, and cardiac MRI were included. The primary endpoint included death, heart failure hospitalization, or life-threatening arrhythmias, with a follow-up of 6 years (5–8). Results: In total, 209 DCM patients were included (aged 54 ± 13 years, 65% male). No associations were observed between collagen volume fraction, circulating carboxy-terminal propeptide of procollagen type-I (PICP), or collagen type I carboxy-terminal telopeptide [CITP] and matrix metalloproteinase [MMP]-1 ratio and cardiac function parameters. However, CITP:MMP-1 was significantly correlated with global longitudinal strain (GLS) in the total study sample (R = −0.40, p < 0.0001; lower CITP:MMP-1 ratio was associated with impaired GLS), with even stronger correlations in patients with LVEF > 40% (R = −0.70, p < 0.0001). Forty-seven (22%) patients reached the primary endpoint. Higher MMP-1 levels were associated with a worse outcome, even after adjustment for clinical and imaging predictors (1.026, 95% CI 1.002–1.051, p = 0.037), but CITP and CITP:MMP-1 were not. Combining MMP-1 and PICP improved the goodness-of-fit (LHR36.67, p = 0.004). Conclusion: The degree of myocardial cross-linking (CITP:MMP-1) is associated with myocardial longitudinal contraction, and MMP-1 is an independent predictor of outcome in DCM patients.
New Technologies in Endourology and Laser Lithotripsy: The Need for Evidence in Comprehensive Clinical Settings
Authors: Candela Luigi , Keller Etienne X. , Pietropaolo Amelia , Esperto Francesco , Juliebø-Jones Patrick , Emiliani Esteban , De Coninck Vincent , Tailly Thomas , Talso Michele , Tonyali Senol , Sener Emre T. , Hameed B. M. Zeeshan , Tzelves Lazaros , Mykoniatis Ioannis , Tsaturyan Arman , Salonia Andrea , Ventimiglia Eugenio
Published: September 2023

Flexible ureteroscopy (fURS) with laser lithotripsy is currently the gold standard surgical treatment for ureteral and kidney stones with a maximum diameter of 2 cm [...]
Changes in Peripheral Blood Eosinophil Counts and Risk of Eosinophilic Granulomatosis with Polyangiitis Onset after Initiation of Dupilumab Administration in Adult Patients with Asthma
Authors: Kushima Yoshitomo , Shimizu Yasuo , Hoshi Hiromi , Arai Ryo , Ikeda Naoya , Nakamura Yusuke , Masawa Meitetsu , Okutomi Hiroaki , Yazawa Nana , Chibana Kazuyuki , Takemasa Akihiro , Niho Seiji
Published: September 2023

Background: The purpose of this study is to clarify the changes in peripheral blood eosinophil (PBE) counts and eosinophilic granulomatosis with polyangiitis (EGPA) onset in patients with asthma who were treated with dupilumab in clinical practice. Methods: The primary outcome of this study is to determine the onset of EGPA in patients whose PBE counts continued to rise within 6 months of dupilumab initiation (rising group) and in patients whose PBE counts peaked and subsequently declined within 6 months (peaked and declined group). As a secondary outcome, the incidence of developing EGPA in patients with PBE counts greater than 1500 cells/μL at 3 or 6 months after dupilumab administration is investigated. Results: A total of 37 individual were enrolled (male/female = 14/23, median age = 57.0 years old). The development of EGPA was significantly more frequent in the rising group compared with the peaked and declined group (p = 0.042, effect size = 0.455, moderate association). Patients with PBE counts greater than 1500 cells/μL showed a significantly higher risk of developing EGPA (p = 0.017, effect size = 0.678, strong association). Conclusions: Physicians should check for the onset of EGPA by monitoring the elevation of eosinophils within 6 months after dupilumab administration, especially in patients with PBE counts greater than 1500 cells/μL at 3 months.
Hypertensive Heart Disease: A Narrative Review Series—Part 2: Macrostructural and Functional Abnormalities
Authors: Nemtsova Valeriya , Burkard Thilo , Vischer Annina S.
Published: September 2023

Hypertensive heart disease (HHD) remains a major global public health concern despite the implementation of new approaches for the management of hypertensive patients. The pathological changes occurring during HHD are complex and involve the development of structural and functional cardiac abnormalities. HHD describes a broad spectrum ranging from uncontrolled hypertension and asymptomatic left ventricular hypertrophy (LVH), either a concentric or an eccentric pattern, to the final development of clinical heart failure. Pressure-overload-induced LVH is recognised as the most important predictor of heart failure and sudden death and is associated with an increased risk of cardiac arrhythmias. Cardiac arrhythmias are considered to be one of the most important comorbidities affecting hypertensive patients. This is the second part of a three-part set of review articles. Here, we focus on the macrostructural and functional abnormalities associated with chronic high pressure, their involvement in HHD pathophysiology, and their role in the progression and prognosis of HHD.
Abuse of Cloud-Based and Public Legitimate Services as Command-and-Control (C&C) Infrastructure: A Systematic Literature Review
Authors: Al lelah Turki , Theodorakopoulos George , Reinecke Philipp , Javed Amir , Anthi Eirini
Published: September 2023

The widespread adoption of cloud-based and public legitimate services (CPLS) has inadvertently opened up new avenues for cyber attackers to establish covert and resilient command-and-control (C&C) communication channels. This abuse poses a significant cybersecurity threat, as it allows malicious traffic to blend seamlessly with legitimate network activities. Traditional detection systems are proving inadequate in accurately identifying such abuses, emphasizing the urgent need for more advanced detection techniques. In our study, we conducted an extensive systematic literature review (SLR) encompassing the academic and industrial literature from 2008 to July 2023. Our review provides a comprehensive categorization of the attack techniques employed in CPLS abuses and offers a detailed overview of the currently developed detection strategies. Our findings indicate a substantial increase in cloud-based abuses, facilitated by various attack techniques. Despite this alarming trend, the focus on developing detection strategies remains limited, with only 7 out of 91 studies addressing this concern. Our research serves as a comprehensive review of CPLS abuse for the C&C infrastructure. By examining the emerging techniques used in these attacks, we aim to make a significant contribution to the development of effective botnet defense strategies.
A Gap Analysis of the Adoption Maturity of Certificateless Cryptography in Cooperative Intelligent Transportation Systems
Authors: Salin Hannes , Lundgren Martin
Published: September 2023

Cooperative Intelligent Transport Systems (C-ITSs) are an important development for society. C-ITSs enhance road safety, improve traffic efficiency, and promote sustainable transportation through interconnected and intelligent communication between vehicles, infrastructure, and traffic-management systems. Many real-world implementations still consider traditional Public Key Infrastructures (PKI) as the underlying trust model and security control. However, there are challenges with the PKI-based security control from a scalability and revocation perspective. Lately, certificateless cryptography has gained research attention, also in conjunction with C-ITSs, making it a new type of security control to be considered. In this study, we use certificateless cryptography as a candidate to investigate factors affecting decisions (not) to adopt new types of security controls, and study its current gaps, key challenges and possible enablers which can influence the industry. We provide a qualitative study with industry specialists in C-ITSs, combined with a literature analysis of the current state of research in certificateless cryptographic in C-ITS. It was found that only 53% of the current certificateless cryptography literature for C-ITSs in 2022–2023 provide laboratory testing of the protocols, and 0% have testing in real-world settings. However, the trend of research output in the field has been increasing linearly since 2016 with more than eight times as many articles in 2022 compared to 2016. Based on our analysis, using a five-phased Innovation-Decision Model, we found that key reasons affecting adoption are: availability of proof-of-concepts, knowledge beyond current best practices, and a strong buy-in from both stakeholders and standardization bodies.
Genomic Based Analysis of the Biocontrol Species Trichoderma harzianum: A Model Resource of Structurally Diverse Pharmaceuticals and Biopesticides
Authors: Al-Salihi Suhad A. A. , Alberti Fabrizio
Published: September 2023

Fungi represents a rich repository of taxonomically restricted, yet chemically diverse, secondary metabolites that are synthesised via specific metabolic pathways. An enzyme’s specificity and biosynthetic gene clustering are the bottleneck of secondary metabolite evolution. Trichoderma harzianum M10 v1.0 produces many pharmaceutically important molecules; however, their specific biosynthetic pathways remain uncharacterised. Our genomic-based analysis of this species reveals the biosynthetic diversity of its specialised secondary metabolites, where over 50 BGCs were predicted, most of which were listed as polyketide-like compounds associated clusters. Gene annotation of the biosynthetic candidate genes predicted the production of many medically/industrially important compounds including enterobactin, gramicidin, lovastatin, HC-toxin, tyrocidine, equisetin, erythronolide, strobilurin, asperfuranone, cirtinine, protoilludene, germacrene, and epi-isozizaene. Revealing the biogenetic background of these natural molecules is a step forward towards the expansion of their chemical diversification via engineering their biosynthetic genes heterologously, and the identification of their role in the interaction between this fungus and its biotic/abiotic conditions as well as its role as bio-fungicide.