bluuebunny/crossref_metadata_2025 huggingface dataset:
In silico structure‐based design of GABAB receptor agonists using a combination of docking and QSAR
Authors: Martínez‐Campos Zuleyma , Pastor Nina , Pineda‐Urbina Kayim , Gómez‐Sandoval Zeferino , Fernández‐Zertuche Mario , Razo‐Hernández Rodrigo Said
Published: June 2019

The study of γ‐aminobutyric acid B receptor (GABAB) activation is of great interest for several brain disorders. The search of new GABAB receptor agonists has been carried out by many research groups. As a result, Baclofen has become the prototypical GABAB receptor agonist. However, several attempts have been made to modify its structure to generate derivatives with improved activity. In this work, we carried out a theoretical and computational study for a wide range of GABAB receptor agonists reported in the literature. Molecular docking and QSAR techniques were combined by using the interaction energies of the agonists with the key residues of GABAB receptor, as molecular descriptors for the QSAR construction. The resulting mathematical model suggests that the activity of GABAB receptor agonists is influenced by three factors: their shape and molecular size (PW5 and PJI2), their constitutional features (ELUMO and T(N…O)) and the energy interaction with GABAB receptor (ETRP278). This model was validated by the QUIK, REDUNDANCY and OVERFITTING rules, and its predicted ability was tasted by the QLOO, QASYM, and rules. Finally, six new compounds are proposed (35–40) with high potential to be used as GABAB receptor agonists.
Parents’ Ethnotheories of Maladaptive Behavior in Young Children
Authors: Olson Sheryl L. , Lansford Jennifer E. , Evans E. Margaret , Blumstein Katherine P. , Ip Ka I
Published: June 2019

Parents’ culturally influenced belief systems, or ethnotheories, are critical components of children’s socialization. Beliefs about children’s desirable characteristics motivate specific parenting activities and moderate the effectiveness of childrearing practices. However, relatively little attention has been given to parents’ ethnotheories of children’s undesirable behavior. From a few studies, we know that parents have culturally specific theories about the nature and management of children’s maladaptive behavior that motivate their socialization practices. In this review, we identify gaps in the research and suggest that qualitative studies of parents’ ethnotheories about the nature and management of children’s deviant behavior have strong theoretical, empirical, and practical benefits for developmental science.
Decreased A20 expression on circulating CD56bright NK cells contributes to a worse disease status in patients with ankylosing spondylitis
Authors: Yang M , Zhou Y , Liu L , Wang S , Jiang J , Shang Q , Yu H , Xiang X , Pang X , Li T , Zhao P
Published: June 2019

A20, a pivotal anti-inflammatory protein, preserves immune homeostasis and regulates prolonged inflammation. A previous study has shown that A20 expression levels are down-regulated in peripheral blood mononuclear cells (PBMCs) from patients with ankylosing spondylitis (AS). However, the precise role of A20 in reducing autoimmune disorders needs to be further elucidated. In this study, A20 expression was found to be preferentially reduced on circulating CD56bright natural killer (NK) cells in patients with AS, and its level was negatively correlated with that of proinflammatory cytokines. Further investigation demonstrated that A20 reduces interferon (IFN)-γ and tumour necrosis factor (TNF)-α production in CD56bright NK cells after stimulation with monokines or phorbol myristate acetate (PMA)/ionomycin(P/I). Furthermore, CD56bright NK cells isolated from AS patients promote TNF-α secretion by autologous monocytes, and increasing the A20 expression level partially attenuates this process. More importantly, decreased A20 expression on circulating CD56bright NK cells is associated with worse disease status in patients with AS. Our findings reveal that A20 participates in the pathogenesis of AS by negatively regulating CD56bright NK cells and that its reduced expression contributes to a worsened disease status in patients with AS.
Obesity in older adults: Effect of degree of weight loss on cardiovascular markers and medications
Authors: Haywood Cilla J. , Prendergast Luke A. , Lim Ratana , Lappas Martha , Lim Wen Kwang , Proietto Joseph
Published: June 2019

Obesity worsens the age‐related tendency towards cardiovascular disease and diabetes. Older adults are vulnerable to medication adverse effects. Intentional weight loss in older adults with obesity has been shown to improve cardiovascular and glycaemic markers. The effect of rapid weight loss induced by very‐low‐calorie diets (VLCDs) on these markers has not been evaluated in this group. In this 12‐week study, participants were randomized to one of healthy eating, hypocaloric diet or VLCD, all combined with three times weekly exercise (Ex/HE, Ex/Diet, Ex/VLCD, respectively). The effects of these interventions on weight, blood pressure, lipids, glucose and HbA1c, inflammatory markers and cardiovascular and diabetes medication changes were measured. Weight loss was 3.7%, 5.1% and 11.1% in Ex/HE, Ex/Diet and Ex/VLCD, respectively. There were significant improvements in HbA1c in all groups, but by the greatest degree in Ex/VLCD (0.18 ± 0.07%, 0.18 ± 0.06% and 0.59 ± 0.13%, respectively). Similar patterns were seen in total cholesterol (0.13 ± 0.15, 0.21 ± 0.11 and 0.53 ± 0.13 mmol/L, respectively, P = .047), triglycerides (0.35 ± 0.13, 0.20 ± 0.10 and 0.51 ± 0.09 mmol/L, respectively, P = .011) and systolic blood pressure (9 ± 2, 2 ± 3 and 14 ± 3 mmHg respectively, P = .025). There were no between‐group differences in fasting glucose, high‐density lipoprotein (HDL) cholesterol, LDL‐C and inflammatory markers. Reductions in anti‐hypertensive or diabetes medication were made in 4/29, 7/36 and 16/37 participants in Ex/HE, Ex/Diet and Ex/VLCD, respectively (P = .017). Significant weight loss achieved with a VLCD gave rise to improvements in multiple cardiovascular risk markers, despite reduction in medication. Weight loss is an under‐utilized method of cardiovascular risk management in this group.
Evaluating approaches for scaling‐up community‐based marine‐protected areas into socially equitable and ecologically representative networks
Authors: Kockel Alessia , Ban Natalie C. , Costa Maycira , Dearden Philip
Published: June 2019

Marine‐protected areas (MPAs) are vital to marine conservation, but their coverage and distribution is insufficient to address declines in global biodiversity and fisheries. In response, many countries have committed through the Aichi Target 11 of the Convention on Biological Diversity to conserve 10% of the marine environment through ecologically representative and equitably managed MPAs by 2020. The rush to fulfill this commitment has raised concerns on how increasing MPA coverage will affect other elements of Target 11, including representation and equity. We examined a Philippines case study to assess and compare 3 MPA planning approaches for biodiversity representation and equitable distribution of costs to small‐scale fishers. In the opportunistic approach, MPAs were identified and supported by coastal communities. The donor‐assisted approach used local knowledge to select MPAs through a national‐scale and donor‐assisted conservation project. The systematic conservation planning approach identified MPA locations with the spatial prioritization software Marxan with Zones to achieve biodiversity objectives with minimal costs to fishers. We collected spatial data on biodiversity and fisheries features and performed a gap analysis to evaluate MPAs derived from different approaches. We assessed representation based on the proportion of biodiversity features conserved in MPAs and distribution equity by the distribution of opportunity costs (fishing areas lost in MPAs) among fisher stakeholder groups. The opportunistic approach did not ineffectively represent biodiversity and resulted in inequitable costs to fishers. The donor‐assisted approach affected fishers disproportionately but provided near‐optimal regional representation. Only the systematic approach achieved all representation targets with minimal and equitable costs to fishers. Our results demonstrate the utility of systematic conservation planning to address key elements of Target 11 and highlight opportunities (e.g., integration of local and scientific knowledge can address representation and equity concerns) and pitfalls (e.g., insufficient stakeholder considerations can exacerbate social inequalities) for planning MPAs in similar contexts.
Iranian Herbalists, But Not Cooks, Are Better at Naming Odors Than Laypeople
Authors: Casillas Marisa , Rafiee Afrooz , Majid Asifa
Published: June 2019

Odor naming is enhanced in communities where communication about odors is a central part of daily life (e.g., wine experts, flavorists, and some hunter‐gatherer groups). In this study, we investigated how expert knowledge and daily experience affect the ability to name odors in a group of experts that has not previously been investigated in this context—Iranian herbalists; also called attars—as well as cooks and laypeople. We assessed naming accuracy and consistency for 16 herb and spice odors, collected judgments of odor perception, and evaluated participants' odor meta‐awareness. Participants' responses were overall more consistent and accurate for more frequent and familiar odors. Moreover, attars were more accurate than both cooks and laypeople at naming odors, although cooks did not perform significantly better than laypeople. Attars' perceptual ratings of odors and their overall odor meta‐awareness suggest they are also more attuned to odors than the other two groups. To conclude, Iranian attars—but not cooks—are better odor namers than laypeople. They also have greater meta‐awareness and differential perceptual responses to odors. These findings further highlight the critical role that expertise and type of experience have on olfactory functions.
Desensitization in heart transplant recipients: Who, when, and how
Authors: Shah Kevin S. , Patel Jignesh
Published: June 2019

The number of heart transplant candidates who have pre‐formed antibodies against human leukocyte antigens (HLAs) is increasing over time. The purpose of this review is to discuss the process of antibody desensitization for heart transplant candidates. Specifically, we review the current status of antibody detection including identification, strength, and potential pathogenicity. We discuss which patients and when should they undergo desensitization therapies during heart transplant evaluation. Specific therapies including mechanical removal of antibodies, intravenous immunoglobulins, and novel immunosuppressive agents targeting antibody production will be discussed. Finally, future research strategies to develop novel desensitization therapies for heart transplant candidates will be reviewed.
The morphologic spectrum of germline‐mutated BAP1‐inactivated melanocytic tumors includes lesions with conventional nevic melanocytes: A case report and review of literature
Authors: Wysozan Timothy R. , Khelifa Sihem , Turchan Kirsten , Alomari Ahmed K.
Published: June 2019

Germline mutations in BRCA1‐associated protein 1 (BAP1) are associated with several neoplasms, including BAP1‐inactivated melanocytic tumors (BIMTs). BIMTs are classically described as biphenotypic melanocytic proliferations with BAP1‐deficient large epithelioid and rhabdoid melanocytes showing various degrees of cytologic atypia. This morphology has been traditionally classified as “spitzoid” despite the various differences between these lesions and the more classic Spitz nevi. Herein, we report a case of an otherwise healthy 11‐year‐old female patient with a family history of several malignancies who presented with multiple pink to brown papules. Histologic and immunohistochemical evaluation identified three lesions with loss of nuclear BAP1 staining. The histologic spectrum of these lesions included junctional spitzoid cells within a triphenotypic proliferation and a separate lesion composed entirely of dermal small to medium‐sized epithelioid melanocytes with maturation. BAP1 gene sequencing revealed a germline frameshift pathogenic BAP1 mutation, denoted c.1717delC. This case provides further evidence that not all BIMTs conform to classic morphological criteria and that the morphologic spectrum includes lesions resembling conventional nevi. As BIMTs can serve as an early marker of the BAP1 hereditary tumor predisposition syndrome, we believe a need exists for a more comprehensive combined clinical and pathological approach for BIMT identification.
Review of a single institution's fine needle aspiration results for thyroid nodules: Initial observations and lessons for the future
Authors: Ronen Ohad , Cohen Hector , Abu Mor
Published: June 2019

Objective

Our objective was to evaluate the disease spectrum of thyroid cytopathology and correlation of the Bethesda reporting system with final histopathology in our medical centre.

Methods

This retrospective study was conducted from histopathology reports from Galilee Medical Center between August 2013 and September 2017.

Results

A total of 287 thyroid fine needle aspirations were included in the study. The majority (55.1%) of these were benign (B2). Surgery was performed on 53 cases and the total malignancy rate was 39.6%. Our study had a favourable accuracy rate of 70%. A B4 Bethesda category had a higher malignancy rate (50%) than previously reported.

Conclusion

We found a higher malignancy rate (50%) based on the Bethesda B4 category of the fine needle aspirations in our series, yet it is based on a small sample. The differences in malignancy rates between centres have an important impact on clinical decisions.
Actions speak louder than words: Differences in memory flexibility between monolingual and bilingual 18‐month‐olds
Authors: Barr Rachel , Rusnak Sylvia N. , Brito Natalie H. , Nugent Courtney
Published: June 2019

Bilingual infants from 6‐ to 24‐months of age are more likely to generalize, flexibly reproducing actions on novel objects significantly more often than age‐matched monolingual infants are. In the current study, we examine whether the addition of novel verbal labels enhances memory generalization in a perceptually complex imitation task. We hypothesized that labels would provide an additional retrieval cue and aid memory generalization for bilingual infants. Specifically, we hypothesized that bilinguals might be more likely than monolinguals to map multiple perceptual features onto a novel label and therefore show enhanced generalization. Eighty‐seven 18‐month‐old monolingual and bilingual infants were randomly assigned to one of two experimental conditions or a baseline control condition. In the experimental conditions, either no label or a novel label was added during demonstration and again at the beginning of the test session. After a 24‐hr delay, infants were tested with the same stimulus set to test cued recall and with a perceptually different but functionally equivalent stimulus set to test memory generalization. Bilinguals performed significantly above baseline on both cued recall and memory generalization in both experimental conditions, whereas monolinguals performed significantly above baseline only on cued recall in both experimental conditions. These findings show a difference between monolinguals and bilinguals in memory generalization and suggest that generalization differences between groups may arise from visual perceptual processing rather than linguistic processing. A video abstract of this article can be viewed athttps://youtu.be/yXB4pM3fF2k
The Pediatric Temporal‐spatial Deviation Index: quantifying gait impairment for children with cerebral palsy
Authors: Zhou Joanne Y , Zhang Kevin , Cahill‐Rowley Katelyn , Lowe Evan , Rose Jessica
Published: June 2019

Aim

To develop an easily‐administered metric to quantify gait impairment in children and to assess its use in children with cerebral palsy (CP).

Method

The Pediatric Temporal‐spatial Deviation Index (TDI) was developed from gait data collected from 75 typically developing children (37 males, 38 females; mean age 9y 4mo; interquartile range [IQR] 8–10y) and 17 children diagnosed with spastic CP (nine males, eight females; mean age 9y 9mo; IQR 9–11y), in Gross Motor Function Classification System (GMFCS) levels I to III, aged 7 to 11 years. Children walked on a pressure‐sensitive mat. Children with CP also completed 3D gait analysis. The Kaiser‐Meyer‐Olkin test of sampling adequacy was used for temporal‐spatial feature selection. Principal components obtained from temporal‐spatial gait parameters quantified deviation from typically developing gait. Deviation was normalized to a Pediatric TDI score mean (standard deviation [SD]) of 100 (10). The Pediatric TDI for children with CP was compared to 3D motion capture‐based Gait Deviation Index (GDI).

Results

The Pediatric TDI was significantly lower for children with CP compared to typically developing children (p<0.001), correlated with average GDI (r=0.610, p=0.009), and demonstrated sensitivity (0.78) and specificity (0.88) to gait function, assessed with GDI.

Interpretation

The Pediatric TDI is an easily administered, revealing gait metric that can be used in children with CP in pediatric clinics and for research. Detection of gait abnormalities in the clinic can expedite diagnosis and treatment.

What this paper adds

The Pediatric Temporal‐spatial Deviation Index (TDI) is a single‐score index of gait deviation, based on nine parameters.

The Pediatric TDI was more revealing than single temporal‐spatial gait parameters.

The Pediatric TDI is quick and simple to administer in the clinic.
Evaluation of left atrial function in type 2 diabetes mellitus patients with nonalcoholic fatty liver disease by two‐dimensional speckle tracking echocardiography
Authors: Chang Wenxing , Wang Ying , Sun Lihua , Yu Dong , Li Ying , Li Guangsen
Published: June 2019

Objective

To assess left atrial function in type 2 diabetes mellitus (T2DM) patients with nonalcoholic fatty liver disease (NAFLD) by two‐dimensional speckle tracking echocardiography (2D‐STE).

Methods

We classified 97 patients with T2DM into three groups according to the results of liver ultrasonography: group A (without NAFLD), group B (mild fatty liver), and group C (moderate to severe fatty liver). Conventional echocardiography parameters included left atrial end‐systolic diameter (LAD), left ventricular end‐systolic and end‐diastolic diameter (LVDs, LVDd), end‐diastolic thickness of ventricular septumi and LV posterior wall (IVSTd, LVPWTd), peak E and A of mitralis (E, A), septal and lateral early (e′) mitral annular diastolic tissue velocities, then calculated E/A and E/mean e′. We measured LV ejection fraction (LVEF) and left atrial (LA) volumes (max, min, and preatrial contraction volume) by Simpson's rule, then calculated LA passive and active ejection fraction (LAPEF, LAAEF), left atrial maximum volume index (LAVImax). The global peak longitudinal systolic strain (LASRs), early diastolic strain (LASRe), and late diastolic strain (LASRa) rates of the LA were obtained by 2D‐STE.

Results

No differences were found between groups A and B (all P > 0.05). In group C, LAAEF and LASRa were obviously higher, while LAPEF, LASRe, and LASRs were obviously decreased compared with those values in groups A and B (all P < 0.05). The association between the severity of NAFLD and the differences in LA strain values remained significant after adjustment for confounders.

Conclusion

Two‐dimensional speckle tracking echocardiography can evaluate the left atrial function in T2DM patients with NAFLD.
Analysis of temporal fluctuations in numbers of sexually mature Anastrepha ludens females over an extensive citrus area in northeastern Mexico
Authors: Vanoye‐Eligio Venancio , de la Luz Vázquez‐Sauceda María , Rosas‐Mejía Madaí , Vera Alicia , Cortés‐Hernández Dora Elia , Rocandio‐Rodríguez Mario
Published: June 2019

The Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae), is one of the most important pests in citrus‐growing areas in Mexico. In this study, we analyzed the temporal fluctuation of sexually mature females of A. ludens populations with respect to the availability of commercial hosts and climatic factors, as well as population data of females over an extensive citrus area in northeastern Mexico. The numbers of mature females were treated as proportions and analyzed with generalized linear mixed models with a binomial error distribution. Results revealed positive effects on proportions of mature females when commercial hosts (Valencia orange and grapefruit) were available at both medium and high levels, whereas negative effects on proportions were observed when commercial hosts were scarcely available. Monthly analyses indicated positive trends on proportion in March, April, October, and November, whereas negative trends were seen in January and June. A low capture of mature females during the late summer and the onset of winter suggests transitional phenological processes across the year. Overall, no relationships were observed between proportions with temperature and the total number of females; however, a positive relationship was observed between proportion and rainfall. These findings provide insight into the reproductive phenology of A. ludens with practical implications for fruit sampling, as well as for the autocidal or chemical control in an areawide integrated pest management (AW‐IPM) approach in citrus‐growing areas of northeastern Mexico.
Presence of Cry1Ab in the Bt maize – aphid (Rhopalosiphum maidis) – ladybeetle (Propylea japonica) system has no adverse effects on insect biological parameters
Authors: Shu Yinghua , Du Yan , Wang Jianwu
Published: June 2019

Transgenic Bacillus thuringiensis Berliner (Bt) crops receive particular attention because they carry genes encoding insecticidal proteins that might negatively affect non‐target arthropods. Here, laboratory experiments were conducted to evaluate the impact of Cry1Ab‐expressing transgenic maize [5422Bt1 (event Bt11) and 5422CBCL (MON810)] on the biological parameters of two non‐target arthropods, the aphid Rhopalosiphum maidis (Fitch) (Hemiptera: Aphididae) and its predator the ladybeetle Propylea japonica (Thunberg) (Coleoptera: Coccinellidae). In a long‐term assay (three generations), no significant differences were found between R. maidis fed Bt maize and those fed a near‐isogenic line (5422) when individual parameters were compared, including nymph development time, adult longevity, aphid spawning period, and fecundity. No negative effects were detected throughout the life cycle of P. japonica in aphids’ feeding amount, development (nymphs, pupae, adults, and progeny eggs), fecundity, or egg hatching when they preyed on Bt maize‐fed aphids compared with non‐Bt maize treatments. A tritrophic assay revealed that Cry1Ab was highly diluted through the food chain (Bt maize leaves, R. maidis, and P. japonica), as detected by an enzyme‐linked immunosorbent assay (ELISA). In conclusion, although Cry1Ab concentrations in maize leaves increased as the plants developed, Cry1Ab levels were significantly reduced in the aphid R. maidis, and no traces of Cry1Ab were detected in P. japonica preying on Bt maize‐fed aphids. The two hybrids of Bt maize expressing Cry1Ab had no negative effects on the measured biological parameters of the aphid R. maidis or its predator, the ladybeetle P. japonica.
Subsaturation of the N‐methyl‐D‐aspartate receptor glycine site allows the regulation of bursting activity in juvenile rat nigral dopamine neurons
Authors: Destreel Geoffrey , Seutin Vincent , Engel Dominique
Published: June 2019

The activation of N‐methyl‐D‐aspartate receptors (NMDARs) in substantia nigra pars compacta (SNc) dopamine (DA) cells is central to generate the bursting activity, a phasic signal linked to DA‐related behaviours via the change in postsynaptic DA release. NMDARs are recruited during excitatory synaptic transmission by glutamate release, but the glycine site level of occupancy of these receptors during basal action potential‐dependent activity is not known for SNc DA neurons. We explored NMDAR‐dependent signals during exogenous applications of co‐agonists in midbrain slices from juvenile rats. We found that both glycine and D‐serine strengthened the NMDAR‐dependent component of excitatory postsynaptic currents (EPSCs) in a concentration‐dependent manner. EPSCs were also increased by endogenous glycine via the blockade of the glycine transport. The glycine site of NMDARs contributing to synaptic transmission is therefore subsaturated. The behaviourally relevant burst firing was more sensitive to exogenous D‐serine and endogenous glycine than to exogenous glycine. The mechanisms regulating the availability of the co‐agonists exert consequently a critical influence on the excitability of DA neurons via NMDARs. The modulation of the phasic firing in DA neurons by ambient NMDAR co‐agonists may be important for nigral information processing and downstream motor‐related behaviour.
Comparison of published risk models for prediction of outcome in patients with extrameningeal solitary fibrous tumour
Authors: Demicco Elizabeth G , Griffin Anthony M , Gladdy Rebecca A , Dickson Brendan C , Ferguson Peter C , Swallow Carol J , Wunder Jay S , Wang Wei‐Lien
Published: June 2019

Aims

Solitary fibrous tumours (SFTs) are fibroblastic mesenchymal tumours with a 10–30% metastatic rate. Several risk models have been proposed for extrameningeal SFT, but they have not been evaluated in direct comparison with each other. The aim of this study is to compare the utility of published risk models in a multi‐institutional SFT cohort.

Methods and results

Clinicopathological data were evaluated for a cohort of extrameningeal SFTs, and used to stratify tumours by the use of five proposed risk models designed for soft tissue and/or pleural SFT [modified Demicco, Pasquali, Salas overall survival (OS), Salas metastasis, and Salas local recurrence (LR)]. Kaplan–Meier and Cox proportional hazards models were used to assess OS, time to first metastasis, time to first LR, and recurrence‐free survival (RFS). The study included 303 patients (109 from a referral cancer treatment centre; previously described in the original Demicco model) and an independent cohort from two large hospitals (n = 194). The median patient age was 54 years, and the median clinical follow‐up (available for 220 patients) was 37 months. The independent cohort had a 13% risk of metastasis at 5 years and a 16% risk of metastasis at 10 years. In this cohort, the modified Demicco, Salas OS, and Salas metastasis models predicted metastasis and RFS, whereas the Pasquali model had the best correlation with OS.

Conclusions

Multivariate risk models that include mitotic rate and patient age can more accurately predict aggressive behaviour in SFTs, with the modified Demicco and Salas OS risk models showing the best correlation with metastasis and RFS.
In Human Immunodeficiency Virus primary infection, early combined antiretroviral therapy reduced γδ T‐cell activation but failed to restore their polyfunctionality
Authors: Casetti Rita , Sacchi Alessandra , Bordoni Veronica , Grassi Germana , Cimini Eleonora , Besi Francesca , Pinnetti Carmela , Mondi Annalisa , Antinori Andrea , Agrati Chiara
Published: June 2019

Primary and chronic human immunodeficiency virus (HIV) infection alters γδ T‐cell features. However, there is no evidence about early combined antiretroviral therapy (cART) and γδ T‐cell dynamics. In the present study, HIV‐positive individuals were divided into those with early primary infection (EPI) and those with late primary infection (LPI). The analysis of γδ T cells was performed by flow cytometry before and after therapy. Polyfunctional profile was assessed after in vitro peripheral blood mononuclear cell (PBMC) exposure to specific antigens. The results show that primary infection induced an expansion of Vδ1 T cells in LPI. Before treatment, a massive activation of γδ T‐cell subsets was observed in both groups of patients, that correlated with disease progression and was significantly reduced after cART introduction. Despite this, CD107A‐expressing Vδ1 T cells in both groups were significantly fewer than in healthy donors, but were restored by therapy introduction. Polyfunctional analysis of Vδ1 T cells from HIV‐positive individuals revealed a lower frequency of CD107A+ CCL‐4+ Vδ1 T‐cell subsets than healthy donors that persists after therapy. Functional profile of Vδ2 was similar to that in healthy donors before therapy but, at 6 months, a lower frequency of CD107A, interferon‐γ‐ or tumor necrosis factor‐α‐producing Vδ2 T cells was observed in the EPI group. Finally, individuals with LPI showed a lower frequency of quadruple‐functional Vδ2 T‐cell subset. In conclusion, during primary HIV infection, the baseline Vδ1 T‐cell activation is correlated with immune reconstitution potential. Moreover, an altered γδ polyfunctional profile occurred, persisting after cART. Further studies are needed to understand whether a longer treatment of primary infection may increase γδ T‐cell functionality.
The association of infant crying, feeding, and sleeping problems and inhibitory control with attention regulation at school age
Authors: Baumann Nicole , Jaekel Julia , Breeman Linda , Bartmann Peter , Bäuml Josef G. , Avram Mihai , Sorg Christian , Wolke Dieter
Published: June 2019

Regulatory problems in infancy and toddlerhood have previously been associated with an increased risk of developing attention problems in childhood. We hypothesized that early regulatory problems are associated with attention problems via reduced inhibitory control. This prospective study assessed 1,459 children from birth to 8 years. Crying, feeding, and sleeping problems were assessed at 5 and 20 months via parent interviews and neurological examinations. At 20 months, inhibitory control was tested with a behavioral (snack delay) task. Attention regulation was assessed at 6 and 8 years using multiple instruments and informants. Detrimental effects of crying, feeding, and sleeping problems on attention regulation were partly mediated by children's ability to inhibit unwanted behaviors (β = −0.04, p = 0.013). Accounting for cognition diminished this indirect effect (β = −0.01, p = 0.209). Instead, the effects of crying, feeding, and sleeping problems on attention regulation were fully mediated by children's cognitive functioning (β = −0.10, p < 0.001). These results support that inhibitory control abilities partly mediate effects of crying, feeding, and sleeping problems. However, these effects may be accounted for by children's general cognitive abilities. Early regulatory problems may set infants on a course of under control of behavior into school age, and such trajectories are highly associated with general cognitive development.
Systems leadership doctor of nursing practice: global relevance
Authors: Chavez F. , Kelly T. , Kunisch J.R. , Kurth A.
Published: June 2019

Aim

This article considers the systems leadership doctor of nursing practice degree as an option to increase nursing leadership roles and heighten presence at policy tables globally, particularly in low‐ and middle‐income countries. Recent global reports emphasize core competencies needed for nursing leadership, particularly systems leadership and health policy, to successfully move the global health agenda forward. Using the Yale University School of Nursing programme as an exemplar, this paper is focused on the elements directly linked to leadership competencies and relevance of a systems leadership doctor of nursing practice programme globally.

Background/Introduction

The dramatic growth and wide variability of doctor of nursing practice programmes offered in all 50 US States have generated questions and debate. According to the American Association of Colleges of Nursing as of May 2018, there are 121 schools that reported having a leadership focus in postmaster's doctor of nursing practice degree offering. Yet there has not been the same enthusiasm for development and implementation for a practice doctorate in nursing across the globe.

Sources of evidence

A narrative literature review was conducted aimed at addressing the relevance of a practice doctorate globally. This analysis of the literature included a search of peer‐reviewed and grey literature. Nursing school websites were visited, and opinions of nurse leaders and students were sought. In addition, global reports that supported nursing leadership and their role in policy development were reviewed.

Discussion/Conclusion

Globally, nurses have a critical role in leading health systems. Developing a cadre of nurse leaders educated at the doctoral level who can communicate with policymakers and develop strategies to meet health systems’ goals is necessary.

Implications for nursing, health and education policy

In recent global health reports and campaigns, strengthening nursing leadership and presence at policymaking tables are recurring themes. Offering a systems leadership doctor of nursing practice degree is one viable option to increase doctorally prepared nurse leaders for nursing policy and practice engagement. This calls for work with country and regional governments, regulatory bodies and nursing associations to empower nursing to contribute fully.
Evaluation of using ICD‐10 code data for respiratory syncytial virus surveillance
Authors: Cai Wei , Tolksdorf Kristin , Hirve Siddhivinayak , Schuler Ekkehard , Zhang Wenqing , Haas Walter , Buda Silke
Published: June 2019

Background

Respiratory syncytial virus (RSV) is the most common cause of acute lower respiratory tract infection (ALRI) in young children. ICD‐10‐based syndromic surveillance can transmit data rapidly in a standardized way.

Objectives

We investigated the use of RSV‐specific ICD‐10 codes for RSV surveillance.

Methods

We performed a retrospective descriptive data analysis based on existing ICD‐10‐based surveillance systems for ALRI in primary and secondary care and a linked virological surveillance in Germany. We described RSV epidemiology and compared the epidemiological findings based on ICD‐10 and virological data. We calculated sensitivity and specificity of RSV‐specific ICD‐10 codes and in combination with ICD‐10 codes for acute respiratory infections (ARI) for the identification of laboratory‐confirmed RSV infections.

Results

Based on the ICD‐10 and virological data, epidemiology of RSV was described, and common findings were found. The RSV‐specific ICD‐10 codes had poor sensitivity 6% (95%‐CI: 3%‐12%) and high specificity 99.8% (95%‐CI: 99.6%‐99.9%). In children <5 years and in RSV seasons, the sensitivities of RSV‐specific ICD‐10 codes combined with general ALRI ICD‐10 codes J18.‐, J20.‐ and with J12.‐, J18.‐, J20.‐, J21.‐, J22 were moderate (44%, 95%‐CI: 30%‐59%). The specificities of both combinations remained high (91%, 95%‐CI: 86%‐94%; 90%, 95%‐CI: 85%‐94%).

Conclusions

The use of RSV‐specific ICD‐10 codes may be a useful indicator to describe RSV epidemiology. However, RSV‐specific ICD‐10 codes underestimate the number of actual RSV infections. This can be overcome by combining RSV‐specific and general ALRI ICD‐10 codes. Further investigations are required to validate this approach in other settings.
Cold atmospheric plasma promotes different types of superficial skin erosion wounds healing
Authors: Gao Jing , Wang Liyun , Xia Chuankai , Yang Xingyu , Cao Zhicheng , Zheng Lei , Ko Randy , Shen Changbing , Yang Chunjun , Cheng Cheng
Published: June 2019

Superficial skin erosion wounds are very common in the clinic, and conventional treatments are not always effective; thus, effective and novel therapy is needed. Cold atmospheric plasma (CAP) has been recognised as a promising approach to wound healing. The purpose of this study is to show the potential clinical application of CAP for the healing of different kinds of superficial skin wounds. Seven patients with different kinds of superficial skin wounds (two patients with pyoderma gangrenosum, two patients with trauma would, one patient with giant genital wart, one patient with diabetic foot, and one patient with chronic eczema) were recruited to this study. All patients accepted and received CAP treatment every other day till the wound healed. The expected results were complete wound healing after CAP treatment. All patients achieved complete wound healing after several rounds (range from two to eight) of CAP treatment, and there was no side effect observed. CAP may provide a new and effective choice to solve the problem of the healing of superficial wounds that are not only caused by trauma but also because of eczema. CAP has certain value in the treatment of superficial skin diseases in the future.
Retracted: microRNA‐374 inhibits proliferation and promotes apoptosis of mouse melanoma cells by inactivating the Wnt signalling pathway through its effect on tyrosinase
Authors: Li Xiao‐Jing , Li Zhi‐Feng , Xu Yan‐Yan , Han Zhao , Liu Zhi‐Jun
Published: June 2019

Melanoma is one of the most malignant skin tumours with constantly increasing incidence worldwide. Previous studies have demonstrated that microRNA‐374 (miR‐374) is a novel biomarker for cancer therapy. Therefore, this study explores whether miR‐374 targeting tyrosinase (TYR) affects melanoma and its underlying mechanism. We constructed subcutaneous melanoma models to carry out the following experiments. The cells were transfected with a series of miR‐374 mimics, miR‐374 inhibitors or siRNA against TYR. Dual luciferase reporter gene assay was used for the verification of the targeting relationship between miR‐374 and TYR. Reverse transcription quantitative polymerase chain reaction and western blot analysis were conducted to determine the expression of miR‐374, TYR, β‐catenin, B‐cell leukaemia 2 (Bcl‐2), Bcl‐2 associated X protein (Bax), Low‐density lipoprotein receptor‐related protein 6 (LRP6), Leucine‐rich repeat G protein‐coupled receptor 5 (LGR5) and CyclinD1. Cell proliferation, migration, invasion, cell cycle distribution and apoptosis were evaluated using cell counting kit‐8 assay, scratch test, transwell assay and flow cytometry respectively. TYR was proved as a putative target of miR‐374 as the evidenced by the result. It was observed that up‐regulated miR‐374 or down‐regulated TYR increased expression of Bax and decreased expressions of TYR, β‐catenin, LRP6, Bcl‐2, CyclinD1 and LGR5, along with diminished cell proliferation, migration, invasion and enhanced apoptosis. Meanwhile, cells with miR‐374 inhibitors showed an opposite trend. These findings indicated that up‐regulated miR‐374 could inhibit the expression of TYR to suppress cell proliferation, migration, invasion and promote cell apoptosis in melanoma cells by inhibiting the Wnt signalling pathway.
Knockdown of LncRNA SCAMP1 suppressed malignant biological behaviours of glioma cells via modulating miR‐499a‐5p/LMX1A/NLRC5 pathway
Authors: Zong Zheqi , Song Yichen , Xue Yixue , Ruan Xuelei , Liu Xiaobai , Yang Chunqing , Zheng Jian , Cao Shuo , Li Zhen , Liu Yunhui
Published: June 2019

Dysregulation of long non‐coding RNAs (lncRNAs) confirm that it plays a crucial role in tumourigenesis and malignant progression of glioma. The present study demonstrated that LncRNA secretory carrier membrane protein 1 (SCAMP1) was up‐regulated and functioned as an oncogene in glioma cells. In addition, miR‐499a‐5p was down‐regulated meanwhile exerted tumour‐suppressive function in glioma cells. Subsequently, inhibition of SCAMP1 significantly restrained the cell proliferation, migration and invasion, as well as promoted apoptosis by acting as a molecular sponge of miR‐499a‐5p. Transcription factor LIM homeobox transcription factor 1, alpha (LMX1A) was overexpressed in glioma tissues and cells. Moreover, miR‐499a‐5p targeted LMX1A 3′‐UTR in a sequence‐specific manner. Hence, down‐regulation of SCAMP1 remarkably reduced the expression level of LMX1A, indicating that LMX1A participated in miR‐499a‐5p‐induced tumour‐suppressive effects on glioma cells. Furthermore, knockdown of LMX1A decreased NLR family, CARD domain containing 5 (NLRC5) mRNA and protein expression levels through directly binding to the NLRC5 promoter region. Down‐regulation of NLRC5 obviously inhibited malignant biological behaviours of glioma cells through attenuating the activity of Wnt/β‐catenin signalling pathway. In conclusion, our study clarifies that SCAMP1/miR‐499a‐5p/LMX1A/NLRC5 axis plays a critical role in modulating malignant progression of glioma cells, which provide a novel therapeutic strategy for glioma treatment.
The toxic effect of cytostatics on primary cilia frequency and multiciliation
Authors: Filipová Alžběta , Diaz Garcia Daniel , Bezrouk Aleš , Čížková Dana , Dvořák Josef , Filip Stanislav , Sturge Justin , Dayanithi Govindan , Šinkorová Zuzana
Published: June 2019

The primary cilium is considered as a key component of morphological cellular stability. However, cancer cells are notorious for lacking primary cilia in most cases, depending upon the tumour type. Previous reports have shown the effect of starvation and cytostatics on ciliogenesis in normal and cancer cells although with limited success, especially when concerning the latter. In this study, we evaluated the presence and frequency of primary cilia in breast fibroblasts and in triple‐negative breast cancer cells after treatment with cytostatics finding that, in the case of breast fibroblasts, primary cilia were detected at their highest incidence 72 hours after treatment with 120 nM doxorubicin. Further, multiciliated cells were also detected after treatment with 80 nM doxorubicin. On the other hand, treatment with taxol increased the number of ciliated cells only at low concentrations (1.25 and 3.25 nM) and did not induce multiciliation. Interestingly, triple‐negative breast cancer cells did not present primary cilia after treatment with either doxorubicin or taxol. This is the first study reporting the presence of multiple primary cilia in breast fibroblasts induced by doxorubicin. However, the null effect of these cytostatics on primary cilia incidence in the evaluated triple negative breast carcinomas cell lines requires further research.
Microbiota of Telenomus tridentatus (Platygastroidea: Scelionidae): An unwanted parasitoid
Authors: de Lourdes Ramírez‐Ahuja María , Gómez‐Govea Mayra A. , Lugo‐Trampe Angel , Borrego‐Soto Gissela , Delgado‐Enciso Iván , Ponce‐Garcia Gustavo , Martínez‐Fierro Margarita L. , Ramírez‐Valles Eda G. , Treviño Víctor , Flores‐Suarez Adriana E. , Rodríguez‐Sanchez Iram P.
Published: June 2019

The microbiota of Hymenoptera is limited to certain bacterial species that vary according to the habitat and diet; however, the bacterial populations are not known in Telenomus tridentatus Johnson & Bin, an egg parasitoid of agricultural importance. We determined the microbiota composition of adults of T. tridentatus using next‐generation sequencing technologies. We found the presence of the Phylums Proteobacteria, in greater proportion followed by Actinobacteria and Firmicutes, respectively. The most abundant species were Cutibacterium acnes, Aquabacterium sp. and Massilia sp. The results of this study could lead to investigating the importance of symbiotic bacteria in T. tridentatus and its relation with agricultural plantations.
Polyphenol oxidase inactivation in viscous fluids by ohmic heating and conventional thermal processing
Authors: Leite Thiago S. , Samaranayake Chaminda P. , Sastry Sudhir K. , Cristianini Marcelo
Published: June 2019

This study investigated polyphenol oxidase (PPO) inactivation in a viscous solution of carboxymethyl cellulose (CMC) by ohmic processing (OH) and conventional thermal processing (CTP) at several temperatures and to assess the impact of CMC consistency on PPO inactivation. OH affects the PPO activity in temperatures higher than 50°C (reduction to 20% at 50°C, 12 min and complete inactivation after 2 min at 80°C), whereas CTP caused a significant reduction in PPO activity only above 60°C (reduction to 90% at 60°C, 8 min and complete inactivation after 2 min at 80°C). The rate of PPO inactivation by ohmic heating was higher than by CTP under all conditions tested, represented by a reduction of D70°C (OH: 3.61 min, CTP 7.32 min), except D80°C was ∼0.5 for both techniques. The Z‐value was higher for OH (14.68°C) than for CTP (8.60°C). Consistency did not have a major impact on inactivation rate.

Practical Applications

Inactivating enzymes that alter quality aspects of food products, such as polyphenol oxidase (PPO), is an important objective of a food processing technology. PPO is an enzyme commonly found in fruits and vegetables that catalyzes browning reactions. Ohmic heating (OH) is a technology known for its potential for processing highly viscous products. This study corroborates the potential of OH for processing highly viscous fluids over conventional thermal processing (CTP), having a great potential to be an alternative technique to food processing. In addition, it gave more evidence of the nonthermal effects due to the electrical current during the ohmic processing.
Comparative semi‐empirical modeling and physico‐functional analysis of hot‐air and vacuum dried puran powder
Authors: Kardile Nilesh Balasaheb , Nema Prabhat Kumar , Kaur Barjinder Pal , Thakre Shubhangi Mahadeo
Published: June 2019

Puran is a stuffing of puran poli (sweet flat bread)—an Indian traditional cuisine made from the mixture of cooked chickpea splits and jaggery. Instant puran powder (IPP) was made by hot air drying (HAD) and vacuum drying (VD) at different temperatures (40, 50, and 60°C) to reduce the preparation time of puran. Different semi‐empirical models were fitted to the drying data and Page model was found to suitably explain the experimental data. The highest water absorption index, water solubility index, rehydration ratio, lightness, firmness, and overall acceptability were found in case of vacuum dried IPP produced at 60°C. Browning index, a*, and b* of vacuum dried IPP at 60°C were the lowest. In vitro protein digestibility and minerals were not significantly affected by the drying method. Highest moisture diffusivity (7.24 × 10−09 m2/s) and lowest activation energy (70.28 kJ/mol) were found in IPP dried by VD at 60°C.

Practical applications

Results of this investigation would facilitate food industries in developing efficient and robust process condition for puran manufacturing. This study is the first report that deals with the physico‐functional properties and drying characteristics of puran. Moreover, models generated in this work for moisture mapping of the product provide vital information for engineering control systems. Vacuum drying technology for puran at 60°C was found the most suitable for commercial preparation of puran powder.