bluuebunny/crossref_metadata_2025 huggingface dataset:
Seroprevalence of the Hepatitis E Virus in Indigenous and Non-Indigenous Communities from the Brazilian Amazon Basin
Authors: Vasconcelos Mariana Pinheiro Alves , de Oliveira Jaqueline Mendes , Sánchez-Arcila Juan Camilo , Faria Sarah Castro , Rodrigues Moreno Magalhães , Perce-da-Silva Daiana , Rezende-Neto Joffre , Pinto Marcelo Alves , Maia-Herzog Marilza , Banic Dalma Maria , Oliveira-Ferreira Joseli
Published: February 2024

Hepatitis E virus (HEV) infection is a common cause of acute viral hepatitis in tropical regions. In Brazil, HEV G3 is the only genotype detected to date. Reports on HEV prevalence are heterogeneous. We aimed to compare the prevalence of anti-HEV among three populations living in the Brazilian Amazon basin. Two cross-sectional studies were conducted in urban, rural, and Yanomami indigenous areas. Plasma samples from 428 indigenous and 383 non-indigenous subjects were tested for anti-HEV IgG using enzyme-linked immunosorbent assays. The overall prevalence of anti-HEV was 6.8% (95%CI: 5.25–8.72), with 2.8% (12/428) found in the Yanomami areas, 3% (3/101) in an urban area, and 14.2% (40/282) in a rural area. Multivariate logistic analysis indicated that patients aged 31–45 years or ≥46 years are more likely to present anti-HEV positivity, with a respective aOR of 2.76 (95%CI: 1.09–7.5) and 4.27 (95%CI: 1.58–12.35). Furthermore, residence in a rural area (aOR: 7.67; 95%CI: 2.50–33.67) represents a relevant risk factor for HEV infection. Additional studies detecting HEV RNA in fecal samples from both humans and potential animal reservoirs are necessary to comprehensively identify risk factors associated with HEV exposure.
Association between Gut Microbiota and Biological Aging: A Two-Sample Mendelian Randomization Study
Authors: Ye Chenglin , Li Zhiqiang , Ye Chun , Yuan Li , Wu Kailang , Zhu Chengliang
Published: February 2024

Recent observational studies revealed an association between gut microbiota and aging, but whether gut microbiota are causally associated with the aging process remains unknown. We used a two-sample Mendelian randomization approach to investigate the causal association between gut microbiota and biological age acceleration using the largest available gut microbiota GWAS summary data from the MiBioGen consortium and GWAS data on biological age acceleration. We further conducted sensitivity analysis using MR-PRESSO, MR-Egger regression, Cochran Q test, and reverse MR analysis. Streptococcus (IVW, β = 0.16, p = 0.0001) was causally associated with Bioage acceleration. Eubacterium (rectale group) (IVW, β = 0.20, p = 0.0190), Sellimonas (IVW, β = 0.06, p = 0.019), and Lachnospira (IVW, β = −0.18, p = 0.01) were suggestive of causal associations with Bioage acceleration, with the latter being protective. Actinomyces (IVW, β = 0.26, p = 0.0083), Butyricimonas (IVW, β = 0.21, p = 0.0184), and Lachnospiraceae (FCS020 group) (IVW, β = 0.24, p = 0.0194) were suggestive of causal associations with Phenoage acceleration. This Mendelian randomization study found that Streptococcus was causally associated with Bioage acceleration. Further randomized controlled trials are needed to investigate its role in the aging process.
Identifying Active Rather than Total Methanotrophs Inhabiting Surface Soil Is Essential for the Microbial Prospection of Gas Reservoirs
Authors: Xu Kewei , Tao Cheng , Gu Lei , Zheng Xuying , Ma Yuanyuan , Yan Zhengfei , Sun Yongge , Cai Yuanfeng , Jia Zhongjun
Published: February 2024

Methane-oxidizing bacteria (MOB) have long been recognized as an important bioindicator for oil and gas exploration. However, due to their physiological and ecological diversity, the distribution of MOB in different habitats varies widely, making it challenging to authentically reflect the abundance of active MOB in the soil above oil and gas reservoirs using conventional methods. Here, we selected the Puguang gas field of the Sichuan Basin in Southwest China as a model system to study the ecological characteristics of methanotrophs using culture-independent molecular techniques. Initially, by comparing the abundance of the pmoA genes determined by quantitative PCR (qPCR), no significant difference was found between gas well and non-gas well soils, indicating that the abundance of total MOB may not necessarily reflect the distribution of the underlying gas reservoirs. 13C-DNA stable isotope probing (DNA-SIP) in combination with high-throughput sequencing (HTS) furthermore revealed that type II methanotrophic Methylocystis was the absolutely predominant active MOB in the non-gas-field soils, whereas the niche vacated by Methylocystis was gradually filled with type I RPC-2 (rice paddy cluster-2) and Methylosarcina in the surface soils of gas reservoirs after geoscale acclimation to trace- and continuous-methane supply. The sum of the relative abundance of RPC-2 and Methylosarcina was then used as specific biotic index (BI) in the Puguang gas field. A microbial anomaly distribution map based on the BI values showed that the anomalous zones were highly consistent with geological and geophysical data, and known drilling results. Therefore, the active but not total methanotrophs successfully reflected the microseepage intensity of the underlying active hydrocarbon system, and can be used as an essential quantitative index to determine the existence and distribution of reservoirs. Our results suggest that molecular microbial techniques are powerful tools for oil and gas prospecting.
Complete Genome Sequences of Four Mycobacteriophages Involved in Directed Evolution against Undisputed Mycobacterium abscessus Clinical Strains
Authors: Cao Yao Juan Carlos , Garcia Cehic Damir , Quer Josep , Méndez Jesús Navas , Gorrín Alexis Dorta , Hevia Lorena García , Fernández María Teresa Tórtola
Published: February 2024

Phage therapy is still in its infancy, but it is increasingly promising as a future alternative for treating antibiotic-resistant bacteria. To investigate the effect of phages on Mycobacterium abscessus complex (MABC), we isolated 113 environmental phages, grown them to high titres, and assayed them on MABC clinical strains through the spot test. Of all the phages, only 16 showed killing activity. Their activity was so temperate to MABC that they could not generate any plaque-forming units (PFUs). The Appelmans method of directed evolution was carried out to evolve these 16 phages into more lytic ones. After only 11 of 30 rounds of evolution, every single clinical strain in our collection, including those that were unsusceptible up to this point, could be lysed by at least one phage. The evolved phages were able to form PFUs on the clinical strains tested. Still, they are temperate at best and require further training. The genomes of one random parental phage and three random evolved phages from Round 13 were sequenced, revealing a diversity of clusters and genes of a variety of evolutionary origins, mostly of unknown function. These complete annotated genomes will be key for future molecular characterisations.
Improvement of Carotenoids’ Production by Increasing the Activity of Beta-Carotene Ketolase with Different Strategies
Authors: Zhou Qiaomian , Huang Danqiong , Yang Haihong , Hong Zeyu , Wang Chaogang
Published: February 2024

Canthaxanthin is an important antioxidant with wide application prospects, and β-carotene ketolase is the key enzyme involved in the biosynthesis of canthaxanthin. However, the challenge for the soluble expression of β-carotene ketolase is that it hinders the large-scale production of carotenoids such as canthaxanthin and astaxanthin. Hence, this study employed several strategies aiming to improve the soluble expression of β-carotene ketolase and its activity, including selecting optimal expression vectors, screening induction temperatures, adding soluble expression tags, and adding a molecular chaperone. Results showed that all these strategies can improve the soluble expression and activity of β-carotene ketolase in Escherichia coli. In particular, the production of soluble β-carotene ketolase was increased 8 times, with a commercial molecular chaperon of pG-KJE8, leading to a 1.16-fold enhancement in the canthaxanthin production from β-carotene. Interestingly, pG-KJE8 could also enhance the soluble expression of β-carotene ketolase derived from eukaryotic microalgae. Further research showed that the production of canthaxanthin and echinenone was significantly improved by as many as 30.77 times when the pG-KJE8 was added, indicating the molecular chaperone performed differently among different β-carotene ketolase. This study not only laid a foundation for further research on the improvement of β-carotene ketolase activity but also provided new ideas for the improvement of carotenoid production.
Synthesis of Silane Functionalized LDH-Modified Nanopowders to Improve Compatibility and Enhance Corrosion Protection for Epoxy Coatings
Authors: Aminifazl Alireza , Karunarathne Darshan Jayasinghe , Golden Teresa D.
Published: February 2024

Novel modified Zn-Al LDH/epoxy coatings are synthesized and applied to steel substrates, providing active corrosion protection and improved barrier properties. This protective coating is made by combining Epon 828 as a polymer matrix with modified layered-double-hydroxy (LDH) nanoparticles acting as corrosion inhibitor containers. To synthesize the coatings, nitrate was intercalated into Zn-Al-LDH layers through an aqueous co-precipitation method to obtain Zn-Al LDH-NO3, and decavanadate replaced nitrate within the LDH layers through an anion exchange process to obtain Zn-Al LDH-(V10O28)6−. The intercalated LDH was functionalized by silanization with (3-aminopropyl)triethoxysilane (APTES) to increase the compatibility of the LDH inhibitor nanocontainers with epoxy resin and produce a protective coating. To protect the mild steel substrate, functionalized LDH nanopowders were dispersed into the epoxy resin, mixed with a polyamide hardener (Epikure 3571), and applied and cured to the metal surface. Surface morphology, structure, and chemical composition were determined for the modified LDH nanopowders using scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis. Corrosion protection of the coating system was studied using long-term immersion testing and potentiodynamic polarization studies in a 3.5 wt.% NaCl solution.
Carlos Gutiérrez-Merino: Synergy of Theory and Experimentation in Biological Membrane Research
Authors: Antollini Silvia S. , Barrantes Francisco J.
Published: February 2024

Professor Carlos Gutiérrez-Merino, a prominent scientist working in the complex realm of biological membranes, has made significant theoretical and experimental contributions to the field. Contemporaneous with the development of the fluid-mosaic model of Singer and Nicolson, the Förster resonance energy transfer (FRET) approach has become an invaluable tool for studying molecular interactions in membranes, providing structural insights on a scale of 1–10 nm and remaining important alongside evolving perspectives on membrane structures. In the last few decades, Gutiérrez-Merino’s work has covered multiple facets in the field of FRET, with his contributions producing significant advances in quantitative membrane biology. His more recent experimental work expanded the ground concepts of FRET to high-resolution cell imaging. Commencing in the late 1980s, a series of collaborations between Gutiérrez-Merino and the authors involved research visits and joint investigations focused on the nicotinic acetylcholine receptor and its relation to membrane lipids, fostering a lasting friendship.
Organic Synthesis and Current Understanding of the Mechanisms of CFTR Modulator Drugs Ivacaftor, Tezacaftor, and Elexacaftor
Authors: Ferreira Filipa , Buarque Camilla , Lopes-Pacheco Miquéias
Published: February 2024

The monogenic rare disease Cystic Fibrosis (CF) is caused by mutations in the gene encoding the CF transmembrane conductance (CFTR) protein, an anion channel expressed at the apical plasma membrane of epithelial cells. The discovery and subsequent development of CFTR modulators—small molecules acting on the basic molecular defect in CF—have revolutionized the standard of care for people with CF (PwCF), thus drastically improving their clinical features, prognosis, and quality of life. Currently, four of these drugs are approved for clinical use: potentiator ivacaftor (VX-770) alone or in combination with correctors lumacaftor, (VX-809), tezacaftor (VX-661), and elexacaftor (VX-445). Noteworthily, the triple combinatorial therapy composed of ivacaftor, tezacaftor, and elexacaftor constitutes the most effective modulator therapy nowadays for the majority of PwCF. In this review, we exploit the organic synthesis of ivacaftor, tezacaftor, and elexacaftor by providing a retrosynthetic drug analysis for these CFTR modulators. Furthermore, we describe the current understanding of the mechanisms of action (MoA’s) of these compounds by discussing several studies that report the key findings on the molecular mechanisms underlying their action on the CFTR protein.
Kaempferol Improves Cardiolipin and ATP in Hepatic Cells: A Cellular Model Perspective in the Context of Metabolic Dysfunction-Associated Steatotic Liver Disease
Authors: Sakurai Akiko , Sakurai Toshihiro , Ho Hsin-Jung , Chiba Hitoshi , Hui Shu-Ping
Published: February 2024

Targeting mitochondrial function is a promising approach to prevent metabolic dysfunction-associated steatotic liver disease (MASLD). Cardiolipin (CL) is a unique lipid comprising four fatty acyl chains localized in the mitochondrial inner membrane. CL is a crucial phospholipid in mitochondrial function, and MASLD exhibits CL-related anomalies. Kaempferol (KMP), a natural flavonoid, has hepatoprotective and mitochondrial function-improving effects; however, its influence on CL metabolism in fatty liver conditions is unknown. In this study, we investigated the effects of KMP on mitochondrial function, focusing on CL metabolism in a fatty liver cell model (linoleic-acid-loaded C3A cell). KMP promoted mitochondrial respiratory functions such as ATP production, basal respiration, and proton leak. KMP also increased the gene expression levels of CPT1A and PPARGC1A, which are involved in mitochondrial β-oxidation. Comprehensive quantification of CL species and related molecules via liquid chromatography/mass spectrometry showed that KMP increased not only total CL content but also CL72:8, which strongly favors ATP production. Furthermore, KMP improved the monolysocardiolipin (MLCL)/CL ratio, an indicator of mitochondrial function. Our results suggest that KMP promotes energy production in a fatty liver cell model, associated with improvement in mitochondrial CL profile, and can serve as a potential nutrition factor in preventing MASLD.
Depressive Symptoms among Bariatric Surgery Candidates: Associations with Stigmatization and Weight and Shape Concern
Authors: Fabrig Alexandra , Schmidt Ricarda , Mansfeld Thomas , Sander Johannes , Seyfried Florian , Kaiser Stefan , Stroh Christine , Dietrich Arne , Hilbert Anja
Published: February 2024

Bariatric surgery candidates (BSC) are a highly vulnerable group for mental health impairments. According to the theoretical model of weight stigma, weight-related experienced stigmatization (ES) negatively influences mental health through weight bias internalization (WBI). This study tested this model among BSC and investigated whether this association depends on a negative body image in terms of weight and shape concern as a potential moderator. As part of a German multicenter study, ES, WBI, weight and shape concern, and depressive symptoms were assessed via self-report questionnaires among n = 854 BSC. Simple and moderated mediation analyses were applied to analyze whether WBI influences the relationship between ES and depressive symptoms, and whether this influence depends on weight and shape concern. WBI significantly mediated the relationship between ES and depressive symptoms by partially reducing the association of ES with depressive symptoms. Weight and shape concern emerged as significant moderators in the overall model and specifically for associations between WBI and depressive symptoms. The results suggest that the association between ES and depressive symptoms among BSC is stronger in those with high WBI. This association is strengthened by weight and shape concern, especially at low and mean levels. Studies evaluating longitudinal associations between weight-related stigmatization and mental health are indicated, as well as intervention studies targeting WBI in order to reduce adverse effects of ES on mental health in BSC.
Background Factors Affecting the Radiation Exposure of the Lens of the Eye among Nurses in Interventional Radiology: A Quantitative Observational Study
Authors: Kuriyama Tomoko , Moritake Takashi , Nakagami Koichi , Morota Koichi , Hitomi Go , Kitamura Hiroko
Published: February 2024

With the International Commission on Radiological Protection’s (ICRP) reduction in the radiation dose threshold for cataracts, evaluating and preventing radiation exposure to the lens of the eye among interventional radiology (IR) staff have become urgent tasks. In this study, we focused on differences in lens-equivalent dose (HT Lens) to which IR nurses in three hospitals were exposed and aimed to identify factors underlying these differences. According to analyses of time-, distance-, and shielding-related factors, the magnitude of the HT Lens dose to which IR nurses were exposed could be explained not by time or shielding but by the distance between the X-ray exposure field and the location of the IR nurse. This distance tended to be shorter in hospitals with fewer staff. The most effective means of reducing the exposure of the lenses of IR nurses’ eyes to radiation is to position them at least two meters from the radiation source during angiography procedures. However, some hospitals must provide IR departments with comparatively few staff. In work environments where it is infeasible to reduce exposure by increasing distance, interventions to reduce time by managing working practices and investment in shielding equipment are also important. This study was not registered.
Completing the Puzzle: A Cluster of Hunting Dogs with Tick-Borne Illness from a Fishing Community in Tobago, West Indies
Authors: Charles Roxanne A. , Pow-Brown Patricia , Gordon-Dillon Annika , Blake Lemar , Nicholls Soren , Brown-Jordan Arianne , Caruth Joanne , Sant Candice , Pargass Indira , Basu Asoke , Albina Emmanuel , Oura Christopher , Georges Karla
Published: February 2024

Eight hunting dogs were visited by a state veterinarian on the island of Tobago, Trinidad and Tobago, West Indies, as owners reported anorexia and paralysis in five of their dogs. The veterinarian observed a combination of clinical signs consistent with tick-borne illness, including fever, anorexia, anaemia, lethargy and paralysis. Blood and ticks were collected from each dog and submitted to a diagnostic laboratory for analysis. Microscopic analysis revealed a mixed infection of intracytoplasmic organisms consistent with Babesia spp. (erythrocyte) and Ehrlichia spp. (monocyte), respectively, from one dog, while a complete blood count indicated a regenerative anaemia (n = 1; 12.5%), non-regenerative anaemia (n = 4; 50%), neutrophilia (n = 3; 37.5%), lymphocytosis (n = 2; 25%), thrombocytopaenia (n = 3; 37.5%) and pancytopaenia (n = 1; 12.5%). DNA isolated from the eight blood samples and 20 ticks (16 Rhipicephalus sanguineus and 4 Amblyomma ovale) were subjected to conventional PCR and next-generation sequencing of the 16S rRNA and 18S rRNA gene for Anaplasma/Ehrlichia and Babesia/Theileria/Hepatozoon, respectively. The DNA of Ehrlichia spp., closely related to Ehrlichia canis, was detected in the blood of three dogs (37.5%), Anaplasma spp., closely related to Anaplasma marginale, in two (25%), Babesia vogeli in one dog (12.5%) and seven ticks (35%) and Hepatozoon canis and Anaplasma spp., in one tick (5%), respectively. These findings highlight the need to test both the vector and host for the presence of tick-borne pathogens when undertaking diagnostic investigations. Further studies are also warranted to elucidate the susceptibility of canids to Anaplasma marginale.
Serological Evidence for Circulation of Influenza D Virus in the Ovine Population in Italy
Authors: Lanave Gianvito , Camero Michele , Coppola Chiara , Marchi Serena , Cascone Giuseppe , Salina Felice , Coltraro Miriana , Odigie Amienwanlen E. , Montomoli Emanuele , Chiapponi Chiara , Cicirelli Vincenzo , Martella Vito , Trombetta Claudia M.
Published: February 2024

Influenza D virus (IDV) is a novel orthomyxovirus initially isolated from pigs exhibiting influenza-like disease in the USA. Since then, IDV has been detected worldwide in several host species, including livestock animals, whilst specific antibodies have been identified in humans, raising concerns about interspecies transmission and zoonotic risks. Few data regarding the seroprevalence of IDV in small ruminants have been available to date. In this study, we assessed the prevalence of antibodies against IDV in ovine serum samples in Sicily, Southern Italy. Six hundred serum samples, collected from dairy sheep herds located in Sicily in 2022, were tested by haemagglutination inhibition (HI) and virus neutralization (VN) assays using reference strains, D/660 and D/OK, representative of two distinct IDV lineages circulating in Italy. Out of 600 tested samples, 168 (28.0%) tested positive to either IDV strain D/660 or D/OK or to both by HI whilst 378 (63.0%) tested positive to either IDV strain D/660 or D/OK or to both by VN. Overall, our findings demonstrate that IDV circulates in ovine dairy herds in Sicily. Since IDV seems to have a broad host range and it has zoonotic potential, it is important to collect epidemiological information on susceptible species.
The Role of the Nuclear Factor-Kappa B (NF-κB) Pathway in SARS-CoV-2 Infection
Authors: Kesika Periyanaina , Thangaleela Subramanian , Sisubalan Natarajan , Radha Arumugam , Sivamaruthi Bhagavathi Sundaram , Chaiyasut Chaiyavat
Published: February 2024

COVID-19 is a global health threat caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is associated with a significant increase in morbidity and mortality. The present review discusses nuclear factor-kappa B (NF-κB) activation and its potential therapeutical role in treating COVID-19. COVID-19 pathogenesis, the major NF-κB pathways, and the involvement of NF-κB in SARS-CoV-2 have been detailed. Specifically, NF-κB activation and its impact on managing COVID-19 has been discussed. As a central player in the immune and inflammatory responses, modulating NF-κB activation could offer a strategic avenue for managing SARS-CoV-2 infection. Understanding the NF-κB pathway’s role could aid in developing treatments against SARS-CoV-2. Further investigations into the intricacies of NF-κB activation are required to reveal effective therapeutic strategies for managing and combating the SARS-CoV-2 infection and COVID-19.
Epidemiology of Ocular Thelaziosis in Domestic Dogs in Beijing
Authors: Liu Zichen , Yu Chang , Tan Xiaoli , Chen Ni , Jin Yipeng
Published: February 2024

Thelazia callipaeda is a zoonotic parasitic nematode that lives in the ocular conjunctival sac of domestic and wild carnivores, lagomorphs, and humans, with Phortica spp. as its intermediate host. At present, the important role that domestic dogs play in thelaziosis has been studied in many countries. However, Beijing, which is the first city in China to experience human thelaziosis, has not yet conducted a comprehensive epidemiological analysis of the disease. In this study, we analyzed risk factors (region, season, age, sex, breed, size, living environment, diet, country park travel history, immunization history, anthelmintic treatment history, and ocular clinical symptoms) associated with the prevalence of thelaziosis in domestic dogs in Beijing. The overall prevalence of T. callipaeda in the study area was 3.17% (102/3215 domestic dogs; 95% CI 2.57–3.78%). The results of the risk factor analysis showed that thelaziosis in domestic dogs from Beijing was significantly correlated with regional distribution, seasonal distribution, country park travel history, and anthelmintic treatment history (p < 0.05). In summer and autumn, domestic dogs living in mountainous areas, with a history of country park travel and without deworming were 4.164, 2.382, and 1.438 times more infected with T. callipaeda than those living in plain areas without a history of country park travel and with a history of deworming (OR = 4.164, OR = 2.382, OR = 1.438, respectively). T. callipaeda-infected domestic dogs did not always show any ocular clinical symptoms, while symptomatic domestic dogs were mainly characterized by moderate symptoms. The results indicate that in summer and autumn, preventive anthelmintic treatment should be strengthened for domestic dogs with a country park travel history or those living in mountain areas. At the same time, we should be vigilant about taking domestic dogs to play in country parks or mountainous areas during summer and autumn because this may pose a potential risk of the owner being infected with T. callipaeda.
Development and Evaluation of a Quantitative Systems Pharmacology Model for Mechanism Interpretation and Efficacy Prediction of Atezolizumab in Combination with Carboplatin and Nab-Paclitaxel in Patients with Non-Small-Cell Lung Cancer
Authors: Wang Chen-Yu , Dai Hao-Ran , Tan Yu-Ping , Yang Di-Hong , Niu Xiao-Min , Han Lu , Wang Wen , Ma Ling-Ling , Julku Aleksi , Jiao Zheng
Published: February 2024

Immunotherapy has shown clinical benefit in patients with non-small-cell lung cancer (NSCLC). Due to the limited response of monotherapy, combining immune checkpoint inhibitors (ICIs) and chemotherapy is considered a treatment option for advanced NSCLC. However, the mechanism of combined therapy and the potential patient population that could benefit from combined therapy remain undetermined. Here, we developed an NSCLC model based on the published quantitative systems pharmacology (QSP)-immuno-oncology platform by making necessary adjustments. After calibration and validation, the established QSP model could adequately characterise the biological mechanisms of action of the triple combination of atezolizumab, nab-paclitaxel, and carboplatin in patients with NSCLC, and identify predictive biomarkers for precision dosing. The established model could efficiently characterise the objective response rate and duration of response of the IMpower131 trial, reproducing the efficacy of alternative dosing. Furthermore, CD8+ and CD4+ T cell densities in tumours were found to be significantly related to the response status. This significant extension of the QSP model not only broadens its applicability but also more accurately reflects real-world clinical settings. Importantly, it positions the model as a critical foundation for model-informed drug development and the customisation of treatment plans, especially in the context of combining single-agent ICIs with platinum-doublet chemotherapy.
New Adverse Drug Reaction Signals from 2017 to 2021—Genuine Alerts or False Alarms?
Authors: Loke Yoon Kong , Mattishent Katharina , Navaneetharaja Navena
Published: February 2024

Spontaneous adverse events reporting systems are used internationally to flag new or unexpected adverse drug reactions (ADRs). Disproportionality analysis is a recognised technique, but false alarms may arise. We aimed to determine whether these new ADR signals had subsequently been followed-up with detailed hypothesis-testing studies. We searched PubMed to identify published studies (years 2017–2021) where the authors reported findings of new ADR signals from disproportionality analyses. We used PubMed and forward citation tracking (Google Scholar) to identify any subsequent confirmatory studies of these ADR signals. We screened 414 titles and abstracts and checked the full-text articles of 57 studies. We found signals for 56 suspected new ADRs from 24 drugs. Google Scholar showed that the ADR studies had been cited a median of seven times (range 0–61). However, none of the suspected new ADRs had undergone detailed evaluation in the citing literature. Similarly, our PubMed search did not find any confirmation studies for the 56 suspected new ADRs. Although many suspected new ADR signals have been identified through disproportionality analysis, most signals have not been further verified as being either genuine ADRs or false alarms. Researchers must focus on follow-up studies for these new signals.
Book Review: Jannel, R. Yamauchi Tokuryū (1890–1982). Philosophie occidentale et pensée bouddhique; Éditions Kimé: Paris, France, 2023; ISBN: 978-2-38072-114-0
Authors: Brenner Joseph E.
Published: February 2024

A recent book by Romaric Jannel on the work of the 20th Century Japanese philosopher Yamauchi Tokuryū is reviewed as a prolegomenon in this journal to more detailed studies of Oriental philosophy. Emphasis is placed on the similarities and overlaps of Eastern and Western thought.
Asymmetrical Cross-Polarization Coupling in a Whispering-Gallery Microresonator
Authors: Sandoval Karleyda , Rosenberger A. T.
Published: February 2024

Cross-polarization coupling between transverse electric (TE) and transverse magnetic (TM) whispering-gallery modes in an optical microresonator produces effects such as coupled-mode induced transparency (CMIT). The detailed analytical theory of this coupling indicates that the TE-to-TM and TM-to-TE couplings may have different strengths. Using an experimental setup centered around a hollow bottle resonator and polarization-sensitive throughput detection, that had been used in previous CMIT experiments, this asymmetry was confirmed and studied. By fitting the throughput spectra of both polarizations to the numerical output of a basic model, the asymmetry parameter defined as the ratio of the coupling amplitudes was determined from the output power in the polarization orthogonal to that of the input. The results of many experiments give a range for this ratio, roughly from 0.2 to 4, that agrees with the range predicted by the detailed theory. An analytical approximation of this ratio shows that the main reason for the asymmetry is a difference in the axial orders of the coupled modes. In some experimental cases, the orthogonal output is not well fitted by the model that assumes a single mode of each polarization, and we demonstrate that this fitting discrepancy can be the result of additional mode interactions.
Compact Diode-Pumped 946 nm Nd:YAG Laser with Good Beam Quality
Authors: Talukder Rubel C. , Major Arkady
Published: February 2024

A continuous-wave (CW), high-power, quasi-three-level Nd:YAG laser operating at 946 nm is reported. The beam quality of the laser is greatly improved. The laser consists of a composite Nd:YAG rod end pumped by a fiber-coupled diode laser inside a simple concave-plane cavity. At an incident pump power of 31 W, a maximum CW output of 9.98 W was obtained at 946 nm, with a beam quality factor of M2~5. The corresponding optical-to-optical efficiency was 32.2% with respect to the incident pump power. To the best of our knowledge, this is the highest output power at 946 nm with such a beam quality ever generated by diode-pumped Nd:YAG laser with bulk crystals.
Deciphering Physio-Biochemical Basis of Tolerance Mechanism for Sesame (Sesamum indicum L.) Genotypes under Waterlogging Stress at Early Vegetative Stage
Authors: Chugh Vishal , Mishra Vigya , Sharma Vijay , Kumar Mukul , Ghorbel Mouna , Kumar Hitesh , Rai Ashutosh , Kumar Rahul
Published: February 2024

Waterlogging represents a substantial agricultural concern, inducing harmful impacts on crop development and productivity. In the present study, 142 diverse sesame genotypes were examined during the early vegetative phase to assess their response under waterlogging conditions. Based on the severity of symptoms observed, 2 genotypes were classified as highly tolerant, 66 as moderately tolerant, 69 as susceptible, and 5 as highly susceptible. Subsequent investigation focused on four genotypes, i.e., two highly tolerant (JLT-8 and GP-70) and two highly susceptible (R-III-F6 and EC-335003). These genotypes were subjected to incremental stress periods (0 h, 24 h, 48 h, 72 h, and 96 h) to elucidate the biochemical basis of tolerance mechanisms. Each experiment was conducted as a randomized split-plot design with three replications, and the statistical significance of the treatment differences was determined using the one-way analysis of variance (ANOVA) followed by the Fisher least significant difference (LSD) test at p ≤ 0.05. The influence of waterlogging stress on morphological growth was detrimental for both tolerant and susceptible genotypes, with more severe consequences observed in the latter. Although adventitious roots were observed in both sets of genotypes above flooding levels, the tolerant genotypes exhibited a more rapid and vigorous development of these roots after 48 h of stress exposure. Tolerant genotypes displayed higher tolerance coefficients compared to susceptible genotypes. Furthermore, tolerant genotypes maintained elevated antioxidant potential, thereby minimizing oxidative stress. Conversely, susceptible genotypes exhibited higher accumulation of hydrogen peroxide (H2O2) and malondialdehyde content. Photosynthetic efficiency was reduced in all genotypes after 24 h of stress treatment, with a particularly drastic reduction in susceptible genotypes compared to their tolerant counterparts. Tolerant genotypes exhibited significantly higher activities of anaerobic metabolism enzymes, enabling prolonged survival under waterlogging conditions. Increase in proline content was observed in all the genotypes indicating the cellular osmotic balance adjustments in response to stress exposure. Consequently, the robust antioxidant potential and efficient anaerobic metabolism observed in the tolerant genotypes served as key mechanisms enabling their resilience to short-term waterlogging exposure. These findings underscore the promising potential of specific sesame genotypes in enhancing crop resilience against waterlogging stress, offering valuable insights for agricultural practices and breeding programs.
Seed Dormancy Class and Germination Characteristics of Prunus spachiana (Lavallée ex Ed.Otto) Kitam. f. ascendens (Makino) Kitam Native to the Korean Peninsula
Authors: Kim Gun Mo , Ko Chung Ho , Chung Jae Min , Kwon Hak Cheol , Rhie Yong Ha , Lee Seung Youn
Published: February 2024

Prunus spachiana (Lavallée ex Ed.Otto) Kitam. f. ascendens (Makino) Kitam leaves exert natural anti-inflammatory effects by inhibiting nitric oxide formation. P. spachiana flowers bloom earlier than other Prunus spp. and thus could serve as a valuable resource for the horticulture and pharmaceutical industries. However, its seed dormancy class and germination traits remain uncharacterized. Thus, this study aimed to characterize the seed dormancy and germination of P. spachiana. Imbibition, phenological, and move-along experiments were performed, and the effects of H2SO4 treatment, hormone soaking, warm/cold stratification, and endocarp removal on germination were explored. Observation revealed that ripe seeds of P. spachiana contain developed embryos and are water permeable. Radicle and shoot emergence began in March and April, respectively, under natural conditions in the year following production. No seed germination was observed after 30 days of incubation at 4, 15/6, 20/10, or 25/15 °C under light/dark conditions, indicating the physiological dormancy of the seeds. Germination increased with prolonged stratification and was affected by incubation temperature. Seed scarification by H2SO4 and soaking with gibberellic acid (GA3) and fluridone were ineffective in breaking dormancy. However, GA3 soaking of the seeds after endocarp removal effectively induced germination (100%). These results indicate that P. spachiana seeds exhibit intermediate physiological dormancy.
G1 Interacts with OsMADS1 to Regulate the Development of the Sterile Lemma in Rice
Authors: Fang Huimin , Chen Hualan , Wang Jianing , Li Ning , Zhang Long , Wei Cunxu
Published: February 2024

Flower development, as the basis for plant seed development, is principally conserved in angiosperms. At present, a number of genes regulating flower organ differentiation have been identified, and an ABCDE model has also been proposed. In contrast, the mechanism that regulates the development of the sterile lemma remains unclear. In this study, we identified and characterized a rice floral organ mutant, M15, in which the sterile lemma transformed into a lemma-like organ. Positional cloning combined with a complementary experiment demonstrated that the mutant phenotype was restored by LONG STERILE LEMMA1/(G1). G1 was expressed constitutively in various tissues, with the highest expression levels detected in the sterile lemma and young panicle. G1 is a nucleus-localized protein and functions as a homomer. Biochemical assays showed that G1 physically interacted with OsMADS1 both in vitro and in vivo. Interestingly, the expression of G1 in M15 decreased, while the expression level of OsMADS1 increased compared with the wild type. We demonstrate that G1 plays a key role in sterile lemma development through cooperating with OsMADS1. The above results have implications for further research on the molecular mechanisms underlying flower development and may have potential applications in crop improvement strategies.
Physiological and Molecular Response of Liriodendron chinense to Varying Stand Density
Authors: Chen Jun , Li Ting , Cai Jinfeng , Yu Pengfei , Guo Ying
Published: February 2024

Stand density affects the potentially superior productivity of forest ecosystems directly by regulating the light and nutrient availability of trees. Understanding how stand density influences the growth and development of trees is crucial for supporting forest management in the context of climate change. We focused on Liriodendron chinense in experimental plantations created in 2003, with planting densities ranging from 277 to 10,000 trees per hectare at six plots. The leaf structure and photosynthetic capacity of L. chinense changed significantly under different stand densities, which had a negative impact on their biomass (leaf mass) and nutrient (total carbohydrate content) accumulation. Transcriptional differences were observed among samples from plots with different planting densities. The expression of 1784 genes was negatively dependent on stand density, participating mainly in the biological processes of “circadian rhythm”, “carbon metabolism”, and “amino acid biosynthesis”. Furthermore, we identified a photosynthesis-related module and constructed a gene regulatory network to discover that the transcription factors of MYB and bHLH may have important roles in the transcriptional regulation of photosynthesis biosynthesis by activating or repressing the expression of petA (Litul.15G096200), psbE (Litul.10G033900), and petD (Litul.17G061600) at different stand densities. Our study quantified the impact of stand density on tree growth at physiological and molecular levels. Our observations provide theoretical support for plantation establishment of L. chinense.
Environmental Assessment of Dryland and Irrigated Winter Wheat Cultivation under Compost Fertilization Strategies
Authors: Amirahmadi Elnaz , Ghorbani Mohammad , Moudrý Jan , Bernas Jaroslav , Mukosha Chisenga Emmanuel , Hoang Trong Nghia
Published: February 2024

Wheat (Triticum aestivum L.) is a strategic agricultural crop that plays a significant role in maintaining national food security and sustainable economic development. Increasing technical performance considering lowering costs, energy, and environmental consequences are significant aims for wheat cultivation. For drylands, which cover approximately 41% of the world’s land surface, water stress has a considerable negative impact on crop output. The current study aimed to assess the environmental aspects of chemical fertilizer in combination with compost in dryland and irrigated winter wheat production systems through life cycle assessment (LCA). The cradle-to-farm gate was considered as the system boundary based on one tone of wheat yield and four strategies: D-C (dryland with compost), D (dryland without compost), I-C (irrigated with compost), and I (irrigated without compost). Based on the results, the highest and lowest amounts of wheat yield were related to the I-C and D strategies with 12.2 and 6.7 ton ha−1, respectively. The LCA result showed that the I strategy in comparison with other strategies had the highest negative impact on human health (49%), resources (59%), ecosystem quality (44%), and climate change (43%). However, the D-C strategy resulted in the lowest adverse effect of 6% on human health, 1% on resources, 10% on ecosystem quality, and 11% on climate change. Utilizing a combination of fertilizer and compost in dryland areas could ensure a higher yield of crops in addition to alleviating negative environmental indicators.
Reddening of the Unicellular Green Alga Euglena gracilis by Dried Bonito Stock and Intense Red Light Irradiation
Authors: Yamashita Kyohei , Hanaki Ryusei , Mori Ayaka , Suzuki Kengo , Tomo Tatsuya , Tokunaga Eiji
Published: February 2024

This study confirms for the first time that the significant red coloration of Euglena gracilis is induced by bonito stock (BS), a traditional Japanese food, and intense red light exposure (605~660 nm, 1000~1300 µmol photons/m2/s). Under the condition, excessive photosynthetic activity destroyed many chloroplasts, while carotenoids were maintained, resulting in the formation of reddened cells. The HPLC analysis revealed that diadinoxanthin was the primary carotenoid present in reddened cells. Additionally, an undefined xanthophyll, not produced under normal culture conditions, was synthesized and suggested to contain a C=O bond. While it has been reported that strong light stress can increase the total carotenoid content of cells, this study did not verify this claim, and it should be investigated further in future research. Under white light irradiation conditions (90 μmol photons/m2/s) in BS medium, no reddening of cells was observed, and good growth was achieved (over four times the cell density in CM medium on the seventh day). This cell suspension is considered to have a high nutritional value because it is composed of functional food, BS and E. gracilis. The fact that this method does not involve genetic modification suggests the possibility of industrial applications, including food use, even in reddened cells.
Advances in Supported Nanoparticle Catalysts
Authors: Carabineiro Sónia Alexandra Correia
Published: February 2024

In catalysis, the design and application of supported nanoparticle catalysts is a cornerstone for addressing contemporary challenges in environmental remediation, sustainable synthesis, and analytical methodologies [...]
Investigation of Heat Transfer Performance in Deionized Water–Ethylene Glycol Binary Mixtures during Nucleate Pool Boiling
Authors: Xu Chen , Ren Jie , Qian Zuoqin , Zhao Lumei
Published: February 2024

Pool boiling heat transfer is recognized as an exceptionally effective method, widely applied across various industries. The adoption of non-azeotropic binary mixtures aligns with the environmental objectives of modern industrial development and enhances the coefficient of performance (COP) in numerous systems. Therefore, investigating the boiling heat transfer characteristics of these mixtures is crucial to improving their industrial usability. In this study, mixtures of ethylene glycol and deionized water (EG/DW) in varying concentrations were chosen as the working fluids. A comprehensive experimental setup was developed, followed by a series of experiments to assess their pool boiling performance. Simultaneously, the thermophysical parameters of these mixtures underwent detailed examination and analysis. The research revealed that the concentration of EG in the mixture markedly affects its thermal properties and temperature glide, both of which are crucial in influencing the heat transfer coefficient. Additionally, six established heat transfer coefficient prediction correlations, primarily designed for pure fluids, have been employed. However, their application to non-azeotropic mixtures under experimental conditions revealed significant deviations. To address this issue, the present study modified existing correlations with the temperature slip characteristics of non-azeotropic mixtures. This process involved recalibrating the wall superheat values in the correlations to reflect the local temperature differential at the boiling point, thereby customizing them for application to non-azeotropic mixtures. The modified correlations highlighted the unique behaviors of non-azeotropic mixtures in boiling heat transfer, demonstrating improved compatibility with these mixtures in a deviation within a permissible 20% range compared with experimental results.
Bearing Fault Diagnosis Method Based on Multi-Domain Feature Selection and the Fuzzy Broad Learning System
Authors: Wu Le , Zhang Chao , Qin Feifan , Fei Hongbo , Liu Guiyi , Zhang Jing , Xu Shuai
Published: February 2024

In recent years, the Broad Learning System (BLS) has been acknowledged for its potential to revolutionize traditional artificial intelligence methods due to its short training time, strong interpretability, and simple structure. In the evolution of BLS, Prof. C. L. Philip Chen’s team introduced the Fuzzy Broad Learning System (FBLS) by replacing the feature nodes of BLS with fuzzy subsystems, thereby further reducing the training time. However, the traditional FBLS, with its straightforward structure, falls short in achieving higher fault diagnosis accuracy when handling raw vibration signals. This paper presents a bearing fault diagnosis approach employing multi-domain feature selection and the fuzzy broad learning system (MS-FBLS), aiming to enhance the diagnostic accuracy of FBLS through multi-domain feature selection. Primarily, a set of 49 features spanning time domain, frequency domain, time-frequency domain, and entropy values is extracted from the original vibrational signals. This combination builds a 49-dimensional multidomain feature set that exploits the information behind the input data as much as possible, thus compensating for the lack of feature extraction capability in FBLS. Afterward, the Random Forest algorithm assesses the significance of all features, leading to a reordering of the multidomain feature set based on their respective importance levels. Ultimately, the reorganized multidomain feature set is then fed into the FBLS, enabling the identification of various failure states within the bearing. The experimental validation conducted on the rolling bearing fault simulation test bed showcased that, in comparison to the traditional FBLS, the MS-FBLS method not only elevates diagnostic accuracy by 23.46%, but also substantially enhances diagnostic speed. These results serve as comprehensive evidence affirming the effectiveness of the method.