bluuebunny/crossref_metadata_2025 huggingface dataset:
Parallel processing of head and whisker motion in two thalamic nuclei
Authors: Ahissar Ehud
Published: May 2023

Rodents move their heads and whiskers continuously, in a coordinated manner, while perceiving objects in their vicinity via whisker-touch. Studies of head-fixed rodents revealed ventroposterior medial (VPM) and posterior medial (POm) thalamic nuclei coding of whisker kinematics1-6, with POm involvement significantly reduced in awake compared to anesthetized animals. In order to examine the involvement of VPM and POm neurons in coding both head and whisker kinematics in head-free conditions, we recorded single-unit activity extracellularly from groups of thalamic neurons along with video tracking of head and whiskers motion in mice freely exploring an open arena. We applied optogenetic tagging to distinguish between neuronal units affiliated with the lemniscal (VPM) or paralemniscal (POm) pathway. Strikingly, in freely moving mice the POm and VPM were involved in whisker kinematic coding to the same degree. In addition, neurons in both thalamic nuclei also robustly coded head kinematics – azimuth, azimuthal velocity and linear speed. The fraction of neurons coding for head kinematics increased in both nuclei after whisker trimming, ruling out whisker-mediated coding of head kinematics. During free exploration, the activity of most thalamic neurons lagged changes in head kinematics, as expected from neurons in a sensory pathway. However, optogenetic activation of thalamic neurons evoked overt changes in head kinematics and increased the fraction of thalamic neurons that were leading changes in head kinematics. Collectively, our data suggest that the VPM and POm both process combined head and whisker information – an integration that is consistent with the tight head and whisker coordination seen during active perception – and participate in the control of head motion.
Exploring the spatially and temporally varying impacts of built environment factors on rail transit ridership: A case study in Nanjing, China
Authors: Wang Chunxin , Hu Mingxing , Qi Junheng , Li Jianyu , Zhang Xinyu , Hu Zihong
Published: May 2023

Abstract: Investigating the impact of the built environment around rail stations on ridership as well as assessing the spatial and temporal variation of that impact is helpful to improving the operational efficiency of urban public transport and guiding station planning and development. Based on 14 days of smart card data in Nanjing, 159 metro stations were taken into consideration in this paper to explain the changing patterns among weekday and weekend ridership. Three types of characteristics were selected as explanatory variables, namely built environment variables, socioeconomic variables and transit service variables. For models, we applied geographically and temporally weighted regression (GTWR) to analyse how the built environment affects ridership from spatial and temporal perspectives and compared its fitting degree in ordinary least squares (OLS) and geographically weighted regression (GWR) models. The results reveal that GTWR performs well in the fitting results and is thus suitable for exploring the association between rail transit ridership and the built environment. In addition, the coefficient of the explanatory variables is influenced by both temporal and spatial factors, with critical importance in the temporal dimension of the analysis of passenger flow. Furthermore, while following the same trend, the impact coefficient, which is mainly affected by commuter traffic, shows stronger temporal heterogeneity during weekdays than weekends. Considering the characteristics of residents' travel activities, the internal relationship between urban space and traffic operation is explored to provide theoretical guidance and suggestions for improving the efficiency of public transport operation and optimizing the layout of urban space.
Immune characteristics associated with lymph node metastasis in early-stage NSCLC
Authors: Zhang Ziyu , Li Li , Gao Yang , Xiao Xiaoxiong , Ji Liyan , Zhou Zhipeng , Jiang Juan , Liu Shiqing , An Jian , Deng Pengbo , Du NanNan , Li Pansong , Zhang Chunliu , Xia Xuefeng , Hu Chengping , Li Min
Published: May 2023

Tumor metastasis is one of the important reasons for the poor prognosis of non-small cell lung cancer (NSCLC) patients, in which lymph node (LN) metastasis is the earliest and most frequent metastasis. With the development of adjuvant immunotherapy, increasing attention has been paid to the tumor-draining lymph nodes（TDLN）in early-stage NSCLC, especially tumor-metastatic lymph nodes, which provides poor prognostic information but has potential benefits in adjuvant treatment. Here, we showed the remodeled immune environment in TDLNs through using TCR-seq and multi-IHC to analyze primary lung cancer tissues and LNs from NSCLC patients with or without LN metastasis. Considering the complex communication between tumor and immunocytes, we subdivided TDLNs and found that metastasis-negative LNs from LN-metastatic patients (MNLN) exhibit more immune activation, exhaustion, and memory compared with both metastasis-positive LNs (MPLN) and TDLNs from non-LN-metastatic patients (NMLN). LN metastasis promotes tumor-specific antigen presentation in TDLNs and induces T cell priming, while existing tumor cells generate an immune-suppressive environment in MPLNs through multiple ways. The evidence implicates the comprehensive immunological mechanism by which LN metastasis influences tumor progression and plays a role in immunotherapy in NSCLC patients.
A Qualitative Study on the Experience of Restructuring Play-centered Curriculum Based on 「2019 Revised Nuri Curriculum」of Early Childhood Special Education Teachers at Kindergarten Special Classes
Authors:   Korean Journal Of Early Childhood Special Education, Kim Yunjeon , Lee Byoungin , Bae Sunghyun
Published: May 2023

The purpose of this study is to find out the experience of restructuring a play-centered curriculum based on the「2019 Revised Nuri Curriculum」through in-depth interviews. To this purpose, in-depth interviews were conducted using semi-structured questionnaires with 4 early childhood special teachers with more than 5 years of experience working in special classes in public kindergartens. As a result of data analysis, 4 upper categories, 16 lower categories, and 49 concepts were derived. The results of this study are summarized as follows. First, early childhood special education teachers recognized themselves as curriculum designers and made efforts in various ways to restructure the play-centered curriculum. Second, early childhood special education teachers restructured the play-centered curriculum through their own practical knowledge and experience, and through this, they had various experiences. Third, the early childhood special education teachers were creating a dynamic but stable curriculum with their children. Fourth, early childhood special teachers demanded various support measures for the reorganization of the curriculum. This study is meaningful in improving the quality of special education for young children with disabilities by examining the process of restructuring the play-centered curriculum by teachers in the「2019 Revised Nuri Curriculum」, which emphasizes teacher autonomy and expertise, and strengthening teachers’ competence.
Verständigung ermöglichen – Eigenständigkeit anregen. Zur Bildungsrelevanz von Online-Lehre in inklusiven Settings
Authors: Redecker Anke
Published: May 2023

Angesichts vielfältiger Kritik am Distanzlernen während der Corona Pandemie, durch das sich viele SchülerInnen allein gelassen fühlten, wird im Beitrag für eine diskursive Bildung plädiert, durch die Lernende mit diversen Voraussetzungen und Bedürfnissen nicht nur von Lehrenden, sondern auch mit- und voneinander lernen können, was einen entscheidenden Beitrag zur Bildungsgerechtigkeit bietet. In der Kritik steht hingegen ein einsames Ableisten von Drill-and-Practice Programmen, das sich auf fremdinitiierte Zumutungen der Selbststeuerung, extrinsische Motivation und vorgezeichnete Lernwege konzentriert und damit wenig Raum für eigensinnige und kreative Erprobungen lässt. Im Ausgang von ihren – auch besonderen – Bedürfnisse, Voraussetzungen und Perspektiven bilden sich Menschen, indem sie angeregt, irritiert und ermutigt werden, sich zu ihren Lerngegenständen und -prozessen ins Verhältnis zu setzen. In digitalen Kontexten bieten hier Videokonferenzen einen tragfähigen Ansatz, sowohl präsenzanalog als auch durch technische Bereicherungen inklusive Bildungsprozesse kokreativ zu gestalten, wobei regelmäßige Videokonferenzen nicht nur Struktur und rituellen Halt bieten, sondern auch vielfältige Möglichketen der Interaktion. Zugleich sind Lernende durch die Bildungsaufgabe herausgefordert, mit digitalen Chancen und Risiken zwischen Ermächtigung und Bemächtigtwerden, eigenständiger Kommunikationsgestaltung und datafizierter Kontrolle, bereichernden Überraschungen und bedrängenden Unwägbarkeiten zurecht zu kommen.
 
AbstractConsidering the manifold critique of distance learning during the Corona pandemic, where many students felt left alone, the text advocates a discoursive education, that enables learners with diverse preconditions and needs to learn not only from teachers, but also with and from each other, which offers a significant contribution to educational justice. Criticized is on the contrary a lonely fulfilling of drill and practice programs which concentrates on externally initiated impositions of self-guidance, extrinsic motivation and predefined learning paths and therefore leaves only little room for self-willed and creative trials. Proceeding from their – even special – needs, preconditions and perspectives, human beings educate themselves being inspired and encouraged to put themselves into relationship to their learning topics and processes. Here video conferences offer a sustainable approach to shape inclusive education processes cocretively in presence analogy and with technical facilities. They enable not only structure and ritual support, but also manifold possibilities of interaction. At the same time learners are challenged by the education task to cope with digital chances and risks between authorization and being dominated, autonomous communicating and dataficated control, enriching surprises and troubling uncertainties.
Body Image, Media Influences, and Situational Dysphoria in Individuals with Visible Physical Disabilities
Authors: Argyrides Marios , Koundourou Christiana , Angelidou Athina , Anastasiades Elly
Published: May 2023

Body image in individuals with visible physical disabilities appears to be an important area of research and investigation, which has received little attention over the years. The purpose of the current descriptive, cross-sectional study was to investigate relationships between the body image construct, possible media influences on body image satisfaction, self-esteem, and the novel variable of situational body image dysphoria in individuals with visible physical disabilities. One hundred fifty-four men and women responded to the measures of interest. Results indicated that appearance satisfaction, weight-related anxiety, pressures from the media, and the internalization of the thin ideal were significant predictors of situational body image dysphoria. Additionally, when controlling for type of disability, females face more difficulties concerning only some of the variables of interest. These findings have important implications for practice with regards to screening measures, the education of healthcare professionals, as well as intervention and rehabilitation programs.
Quality of working life of scientific and pedagogical workers: determinants and evaluation indicators
Authors: Riabokon Ivan , Shandar Anna
Published: May 2023

The new realities caused by military actions as a result of russian aggression have led to adverse consequences for the Ukrainian economy, which negatively affects the state of higher education, in particular the quality of working life of workers in this area. In such circumstances, Ukraine needs to take measures to improve the quality of the working life of scientific and pedagogical workers and improve the methodology for its assessment. The purpose of the paper is to theoretically substantiate the determinants of the quality of the working life of scientific and pedagogical workers and to provide a scientific and methodological justification for the system of indicators for its assessment. The study analyzes scientific approaches to the concept, essence and assessment of the quality of the working life of scientific and pedagogical workers. It also classifies the determinants of the quality of their working life, the main of which are financial, organizational and legal, managerial, and security. The following key components of the quality of working life are identified: compensatory (material), “decent working conditions”, “social security”, “development and self-realization”. The author’s approach to understanding the concept of the quality of working life of scientific and pedagogical workers and its assessment is presented. Based on the principles of the Strategy for the Development of Higher Education in Ukraine for 2021–2031, compiled by the Ministry of Education and Science of Ukraine, a system of indicators of the quality of working life of scientific and pedagogical workers has been formed that correspond to its strategic goals and operational tasks.
Four Brazilian Potential Species Almost Unexplored of Connaraceae Family Used Popularly for Diabetes: Chemical Composition and in vitro Activity
Authors: Paim Luís Fernando , Acho Leonard , dos Santos Paulo Roberto , Toledo Cássio Augusto , Minello Luana , da Paz Joicelene Regina , Lima Emerson , Salvador Mirian , Moura Sidnei
Published: May 2023

Connaraceae is a plant family usually used in traditional medicine. Recently, we have reported the chemical composition and biological activity of 39 species around the world. This work highlights the application of these species to diabetes control, which has been reported at least to half of this family. In this line, there are several species of the Connaraceae without scientific evidence of traditional use, among these the four species study here. Thus, this work aims the chemical composition prospection of Connarus blanchetii, Connarus regnellii, Connarus suberosus and Rourea glazioui, as well as the identification of possible bioactivity. The leaves of these four species were collected, prepared and submitted to different extractive methods (aqueous decoction and infusion and ethanolic maceration), these being tested in a preliminary phytochemical investigation and subjected to antioxidant assay with 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power. Then, we selected the maceration extract to continue for fractions, with the ethyl acetate and n-butanol presenting the best results with antioxidant and anti-advanced glycation end products (AGEs) activity potential. Finally, the chemical composition was determinate by high-resolution mass spectrometry (HRMS) in combination with Global Natural Products Service (GNPS) data bank. As a result, this study indicated the presence of 29 phenolics compounds with reported activity as antioxidant and with potential of protein glycation, supported the diabetes activity of this plants. The n-butanolic fraction of R. glazioui showed compounds such as apigenin, kaempferol, quercetin, myricetin and chlorogenic acid, which have generated a half maximal inhibitory concentration (IC50) of 36.5 µg mL-1 for oxidative glycation inhibition, being highlighted as the most active.
An improved technique for reducing water wastage from micro-RO-membrane-based water purification systems: An experimental study
Authors: Varma Ravi Kiran , Chatterjee Jaideep
Published: May 2023

While reverse osmosis (RO) has emerged as the leading technology for home-use and point-of-use (POU) water purification, this technology leads to water wastage as the reject. This wastage needs to be reduced, especially in water-stressed regions of the world. In this paper, we report an experimental study, which demonstrates that it is possible to operate a home-use RO-based water purification system, at ∼75% recovery, without significantly compromising the life of the membrane. The membrane element is ‘cleaned’ using a hydrostatic and osmotically driven backwash using the permeate produced in the system. This study examines the effect of the permeate volume used for the backwash, on the extent of salt removal from the membrane module. During this study, which involved the measurement of the total dissolved solids (TDS) of the backwash-effluent, it was observed that the backwash-effluent showed dual-minima in the effluent TDS. This paper further examines this dual-minima observed under different experimental conditions. Data generated suggest the causes for the dual-minima.
Mundialização da cultura: notas sobre inovação e desenvolvimento cultural
Authors: Lima Wesley , José Rogério Lopes
Published: May 2023

O presente texto busca dimensionar uma discussão teórica acerca de temas relacionados à cultura em seus variados aspectos da vida cotidiana. Verificou[1]se, com base na pesquisa bibliográfica com síntese qualitativa, os aspectos sociais e políticos que levaram a cultura a se tornar mundializada e, consequentemente, consumível. Ademais, visou-se articular a essa problematização o papel da cultura como um elemento agenciador de inovação e, portanto, de desenvolvimento. As discussões realizadas demonstraram que a globalização e a emergência de uma sociedade global acarretaram uma massificação da cultura, transfigurando-a em um elemento cabível de ser adquirido, ao mesmo tempo em que sua mundialização produziu transformações substanciais nas sociedades, por meio da inovação técnica e da indústria cultural.
Por uma educação patrimonial emancipadora
Authors: Furtado Henrique
Published: May 2023

O presente artigo discute acerca da educação patrimonial enquanto prática emancipadora. Para tanto, pensamos a respeito de sua definição, bem como da necessidade de refletir sobre seu caráter educacional e suas possibilidades pedagógicas para o ensino mediante a produção de diálogos entre a educação patrimonial e a pedagogia libertadora de Paulo Freire
A constituição do arquivo público no município de Picada Café: preservação da memória histórica e cultural da cidade
Authors: Führ Jean Jeison , Gasparetto Quésia Katúscia , Ferreira Glauber West , Anelise Ruppenthal Anelise Ruppenthal Trierweiler
Published: May 2023

O objetivo deste artigo é efetuar o relato de experiência da constituição do Arquivo Público do Município de Picada Café (RS). Tem-se buscado em Picada Café a preservação da memória histórica e cultural da localidade por meio da instituição de um novo espaço arquivístico que não somente abarque as necessidades de acesso à informação por parte de sua população, mas que também possibilite a garantia de preservação do patrimônio documental da cidade. Antes de adentrar no relato propriamente dito, principiamos por um debate conceitual da relação entre História e Arquivologia e seguimos por reflexões sobre a realidade dos arquivos públicos em esfera municipal. Por fim, ao efetuarmos o relato da constituição em andamento do Arquivo Público (e Histórico) do Município de Picada Café, evidenciamos as constatações diagnósticas sobre o que a estratégia de estabelecimento desse espaço social poderá proporcionar aos cidadãos cafeenses.
Ansiedade no tratamento odontológico: estudo exploratório com crianças e adolescentes de um município de Santa Catarina
Authors: Elisabete Rabaldo BOTTAN  , Gabrielly Ludwig LEHMKUHL  , Silvana Marchiori ARAÚJO
Published: May 2023

Introdução: Estudos clínicos e de levantamento de dados evidenciamum interesse crescente sobre a temática da prevalência da ansiedadeno tratamento odontológico e sua influência no desenvolvimento dotrabalho do cirurgião-dentista, pois, apesar de todos os avançostecnológicos na área da odontologia, o medo do tratamento odontológicocontinua sendo uma significativa barreira à otimização dos serviços desaúde bucal. Objetivos: Determinar o percentual de sujeitos comansiedade quanto ao tratamento odontológico e caracterizar os sujeitosclassificados como portadores de alto grau de ansiedade, em uma população de estudantes do ensino fundamental. Material e métodos:Estudo descritivo, do tipo transversal, com alunos matriculados emquatro escolas do ensino fundamental localizadas no perímetro urbanode um município do Alto Vale do Itajaí (SC). A amostra não probabilísticafoi constituída de 797 escolares que responderam a dois questionários,elaborados com base nas escalas Dental Anxiety Scale (DAS) e DentalFear Survey (DFS). Resultados: Foram classificados como ansiosos87% dos pesquisados, no entanto a maioria era portadora de baixaansiedade. As meninas demonstraram ser mais ansiosas do que osmeninos. As respostas fisiológicas mais relatadas perante o tratamentoodontológico foram tremores e aceleração dos batimentos cardíacos.Os fatores desencadeadores de medo para as meninas foram dentistae cadeira do dentista e, para os meninos, broca e agulha. A maior partedos sujeitos pesquisados afirmou ter visitado o dentista nos dois últimosanos para consultas do tipo curativo. Conclusão: A freqüência desujeitos com algum grau de ansiedade quanto ao tratamento dental nogrupo pesquisado é alta, quando comparada a outros grupos.
Avaliação da adaptação marginal de coroa de resina reforçada com fibra de vidro em microscópio eletrônico de varredura
Authors: Marcio José Fraxino BINDO  , Rogério Goulart da COSTA  , Eduardo Christiano Caregnatto de MORAIS  , Moira Pedroso LEÃO  , Sávio Marcelo Leite MOREIRA DA SILVA
Published: May 2023

Introdução e objetivo: As próteses fabricadas com resinas reforçadassão alternativas para o tratamento reabilitador de um ou mais elementosdentários. Objetivando prever a longevidade dessas peças, este trabalhoavaliou a adaptação marginal de uma coroa total confeccionada com resinareforçada com fibra de vidro. Material e métodos: Um dente com indicaçãoortodôntica de exodontia foi preparado e sobre ele uma peça protética foicimentada para que, 48 horas depois, a extração fosse executada. Oconjunto foi levado ao laboratório para leitura em microscópio eletrônicode varredura (MEV). Foram feitas 30 leituras em uma interface de dente–cimento–restauração de 8 mm e reveladas diferentes gaps em toda aamostra. Resultados: O menor valor achado foi de 15,82 µm, e o maiorde 72,81 µm, resultando em uma média de 40,82 µm. Conclusão: Asgaps estavam dentro dos limites de tolerância clínica e, considerandoa dificuldade clínica de produção da peça protética, o tratamentomostrou-se satisfatório no que concerne à adaptação marginal.
Avaliação da estabilidade dimensional de alguns cimentos endodônticos nacionais contendo óxido de zinco e eugenol
Authors: Fernando Marques da CUNHA  , Rosiléa Dassié Pansini GRANEIRO  , Sandra Rivera FIDEL  , Rivail Antônio Sérgio FIDEL
Published: May 2023

Introdução e objetivo: A estabilidade dimensional dos cimentosendodônticos contendo óxido de zinco e eugenol Endofill®, Intrafill®,Cimento de Rickert®, Pulp-Fill® e Target® foi avaliada mediante o uso daespecificação n.º 57 da American Dental Association. Material e métodos:A estabilidade foi avaliada por meio da média aritmética de 5 medições,realizadas com paquímetro, do comprimento dos corpos-de-prova, antese após estes terem sido imersos por 30 dias em recipientes com águadestilada e deionizada a 37°C. Resultados: Os resultados foramanalisados estatisticamente pelos testes de Shapiro-Wilk, Kruskal-Wallise Mann-Whitney no nível de significância de 5%. Os cimentos Endofill®,Cimento de Rickert® e Pulp-Fill® demonstraram contração de acordocom o preconizado. Conclusão: Apesar de apresentarem resultadoscompatíveis com a necessidade clínica, os cimentos Target® e Intrafill®tiveram expansão maior do que a preconizada pela especificação 57.
Avaliação da capacidade de dissolução de tecido pulpar bovino pelo ácido tricloroisocianúrico nas concentrações de 1%, 2%, 3% e 4% comparativamente ao hipoclorito de sódio 1%
Authors: Celeste Blauth JUCHEM  , Gabriela Barbosa PEREIRA  , Renata Grazziotin SOARES  , Luis Eduardo Duarte IRALA  , Alexandre Azevedo SALLES  , Orlando LIMONGI
Published: May 2023

Introdução e objetivo: Em função de a dissolução tecidual ser umfator relevante para o saneamento dos canais radiculares, este estudoavaliou a capacidade do ácido tricloroisocianúrico nas concentraçõesde 1%, 2%, 3% e 4% de dissolver tecido pulpar bovinocomparativamente ao hipoclorito de sódio 1%. Material e métodos:Utilizaram-se seis polpas de incisivos inferiores bovinos com média de 13 mm de comprimento. As polpas foram colocadas em copos de Beckerde 20 mL que continham 10 mL das soluções testadas. Foram utilizadoso hipoclorito de sódio 1% como controle positivo e a água destiladacomo controle negativo. Resultados e conclusão: Em 2 horas e 29minutos obteve-se dissolução total do tecido pulpar com a utilizaçãodo NaOCl 1%, e nenhuma dissolução foi verificada com a água destilada.Em um tempo de 12 horas de observação o ácido tricloroisocianúriconão foi capaz de dissolver a totalidade de tecido. Os resultados em 12horas de observação foram: ácido tricloroisocianúrico 1% dissolveu47%, ácido tricloroisocianúrico 2% dissolveu 54%, ácidotricloroisocianúrico 3% dissolveu 62% e ácido tricloroisocianúrico 4%dissolveu 77% de tecido pulpar. Em relação ao ácidotricloroisocianúrico, mais pesquisas devem ser realizadas, levando emconsideração, além do tempo para dissolução do tecido pulpar, osefeitos que esse ácido exerce nos tecidos periapicais.
Educação em saúde bucal: análise do conhecimento dos professores do ensino fundamental de um município da região do Vale do Itapocu (SC)
Authors: Josymeire VASEL  , Elisabete Rabaldo BOTTAN  , Luciane CAMPOS
Published: May 2023

Introdução: A escola tem sido considerada um local adequado para odesenvolvimento de programas de saúde. A possibilidade de implantarações dessa natureza foi o fator propulsor desta pesquisa. Objetivo:Buscou-se avaliar conhecimentos e atitudes de professores do ensinofundamental de um município de Santa Catarina sobre saúde e higieneoral. A investigação constou de um estudo descritivo, do tipo transversal,mediante levantamento de dados por meio de um questionário. Apopulação-alvo foram 115 professores de 1.ª a 5.ª série, da qual foiconstituída uma amostra não probabilística (n=74), representando64,34% da população-alvo. Para determinar o nível de conhecimento foi criada uma categorização específica, classificando-o em bom, regulare insatisfatório. Resultados: Os pesquisados têm dificuldade de acessoa informações, conteúdos e materiais relativos à saúde bucal, pois essestemas geralmente não são discutidos nos cursos de atualização. Amaioria não possui conhecimentos específicos suficientes para realizarum trabalho de educação em saúde bucal com seus alunos, pois apenas8% se classificaram como portadores de bom nível de conhecimento.Conclusão: É necessário fornecer subsídios teórico-práticos a esseseducadores para que possam tratar de tais conteúdos de formaadequada. Para tanto, é preciso estimular a adoção de novas estratégiase a formação continuada, mediante um trabalho multiprofissional queenvolva a participação ativa dos cirurgiões-dentistas e dos professoresdo ensino fundamental.
Tempo de endurecimento de alguns cimentos endodônticos à base de óxido de zinco e eugenol
Authors: Rivail Antonio Sérgio FIDEL  , Rosiléa Dassié Pansini GRANEIRO  , Luciana Carvalho REIS  , Viviane Dias Martini Alves NASCIMENTO  , Sandra Rivera FIDEL
Published: May 2023

Objetivo: Procurou-se analisar ex vivo o tempo de endurecimento doscimentos endodônticos nacionais à base de óxido de zinco e eugenol:Endofill®, Intrafill®, cimento de Rickert (LenzaFarm®), Pulp Fill® eTarget®. Métodos: O tempo de endurecimento dos cimentosendodônticos foi obtido de acordo com a especificação n.º 57 daAmerican Dental Association. Resultados: A análise das médias indicoua existência de diferença altamente significante entre os tempos deendurecimento dos cimentos. Conclusão: Considerando a propriedadeavaliada, os cimentos Target® e Intrafill® mostraram valores maiscompatíveis com a atividade clínica.
Apical and periapical tissues responses after root canal obturation with two calcium hydroxid based sealers in dog’s teeth
Authors: Maria Renata Giazzi NASSRI  , Antonio Carlos BOMBANA  , Raphael Carlos Comelli LIA
Published: May 2023

Introduction: At the ending of endodontic treatment, is very importantthat the choice of root canal’s sealer will be done by biologicalcompatibility criterial, which could promote less inflammation, turningquicker and more effective the healing process. Objective andmethodology: The aim of this study was to evaluate the apical andperiapical tissues reactions in dog’s teeth, after pulpectomy and rootcanal’s cleaning and shaping, which limit was the fisiological barrier ofdelta apical, and filling with gutta-percha and two calcium hydroxidebased endodontics sealers, Sealapex® and Apexit®, by thosehystopatological events in observation periods of 7, 21 and 45 days,making a comparison of inflammatory reactions and reparacionalevolution that both materials promoted. Past all periods, the dogs werekilled and histological cuts of teeth were obtained. Results: Through qualitative-quantitative analysis, Sealapex showed inflammatory levelamong mild and moderate in all periods, having a great improvementin healing process at 45 days. There was a biggest dispersion of Apexitin periapical tissue, which caused an intense macrophage activity,raising inflammatory level in last period. Conclusions: Regardless ofthe sealer used, there were observed necrosis in a few of delta apical’sramifications, being mostly in tooth filling by Apexit. In both ways,Sealapex showed best results than Apexit, about increased inflammatoryreaction and healing process.
Comparative histological study between MTA and Portland cement
Authors: Cristina de Jesus REISS-ARAÚJO  , Kércya da Silva PAIM  , Marcelo de Azevedo RIOS  , Diana Santana de ALBUQUERQUE  , Flares BARATTO FILHO  , José Roberto VANNI
Published: May 2023

Introduction: Several studies have demonstrated the effectiveness ofMTA as a promising material in Dentistry. There is a possibility thatthe Portland cement may provide the same results of MTA due to thesimilar composition of both materials. Objective: comparing the MTAand Portland cement by means of histological observation. Materialand methods: Two groups of five wistar rats (Rattus novergicus) eachwere submitted to surgery at the filtrum area for insertion of apolyethylene tube containing MTA (left side) and Portland cement (rightside). After flap closure, the animals were placed in isolation and twodifferent periods (2 and 12 weeks) were selected to sacrifice the animals.Blocks were obtained from the animals, stored in 10% formalin solutionand submitted to histological preparation. All blocks were observedunder a light microscope. Results: The results showed that the MTAyielded a less intense histological reaction than the Portland cement in 2-week period. In the 12-week period, the histological results werevery similar for both cements. Conclusion: Utilization of the Portlandcement in Dentistry can be considered.
O uso do vidro bioativo na terapia regenerativa periodontal – revisão da literatura
Authors: Bahjat NASSER NETO  , Tatiana Miranda DELIBERADOR  , Carmen Lucia Mueller STORRER  , Andrea Maria de SOUSA  , Tertuliano Ricardo LOPES
Published: May 2023

Introdução e objetivo: A correção dos defeitos ósseos causados peladoença periodontal é realizada por intermédio de procedimentoscirúrgicos que podem ser divididos como ressectivos, reparativos ouregenerativos. O objetivo deste trabalho é revisar e discutir a literaturarelativa ao uso do vidro bioativo nos procedimentos periodontaisregenerativos. Revisão de literatura e conclusão: Os procedimentosregenerativos correspondem a formas de tratamento que visam reproduzirou reconstituir a parte perdida ou injuriada dos tecidos periodontais. Aregeneração periodontal pode ser definida como o procedimento que buscaregenerar os tecidos de suporte do dente, formados por osso alveolar,cemento e ligamento periodontal. Numerosas modalidades cirúrgicas têmsido testadas para alcançar a regeneração dos defeitos ósseos periodontais.Os métodos mais comumente utilizados são os enxertos ósseos autógenos,alógenos e xenógenos, a regeneração tecidual guiada (RTG) e o uso de materiais aloplásticos (substitutos ósseos). Entre os materiaisaloplásticos está o vidro bioativo (VB), o qual é definido como umacerâmica bioativa que se caracteriza por ser osteocondutora eosteoestimulatória e por ter a propriedade de adesão óssea. Algunsestudos histológicos mostram que o uso dos vidros bioativos podeinduzir a neoformação de cemento e a formação de uma nova inserção.
Global Xenobiotic Profiling of Rat Plasma Using Untargeted Metabolomics and
Background Subtraction-Based Approaches: Method Evaluation and Comparison
Authors: Jiang Xiaojuan , Chen Simian , Zhu Mingshe , Wu Caisheng
Published: May 2023

Background:

Global xenobiotic profiling (GXP) is to detect and structurally characterize all xenobiotics
in biological samples using mainly liquid chromatography-high resolution mass spectrometry (LC-HRMS) based
methods. GXP is highly needed in drug metabolism study, food safety testing, forensic chemical analysis, and exposome
research. For detecting known or predictable xenobiotics, targeted LC-HRMS data processing methods based on
molecular weights, mass defects and fragmentations of analytes are routinely employed. For profiling unknown xenobiotics,
untargeted and LC-HRMS based metabolomics and background subtraction-based approaches are required.

Objective:

This study aimed to evaluate the effectiveness of untargeted metabolomics and the precise and thorough
background subtraction (PATBS) in GXP of rat plasma.

Methods:

Rat plasma samples collected from an oral administration of nefazodone (NEF) or Glycyrrhizae Radix et
Rhizoma (Gancao, GC) were analyzed by LC-HRMS. NEF metabolites and GC components in rat plasma were thoroughly
searched and characterized via processing LC-HRMS datasets using targeted and untargeted methods.

Results:

PATBS detected 68 NEF metabolites and 63 GC components, while the metabolomic approach (MS-DIAL)
found 67 NEF metabolites and 60 GC components in rat plasma. The two methods found 79 NEF metabolites and 80
GC components with 96% and 91% successful rates, respectively.

Conclusion:

Metabolomics methods are capable of GXP and measuring alternations of endogenous metabolites in a
group of biological samples, while PATBS is more suited for sensitive GXP of a single biological sample. A combination
of metabolomics and PATBS approaches can generate better results in the untargeted profiling of unknown
xenobiotics.
Recent Studies on Serotonin 5-HT2A Receptor Antagonists in Medicinal
Chemistry: A Last Decades Survey
Authors: Kamadar Jignesh H. , Kumari D Roza , Kapadiya Khushal M
Published: May 2023

In the last decades, much attention has been paid to the functioning of receptors to
understand better how they work with various chemical motifs. Among different families, G-proteincoupled
receptor (GPCR) families have drawn much attention in the twenty-first century. They are the
most prominent signal transducer across the cell membrane, comprising thousand-odd proteins. One
of the members of GPCRs is the serotonin 2A (5-HT2A) receptor, which has been associated with
complex etiological mental illnesses. In this survey, we collected data on 5-HT2A, i.e., the role of 5-
HT2A receptors in human and animal analogy, various binding site functionalities, advanced effects,
and synthetic aspects.
Higher Abundance of Vitronectin (S-protein) in Serum-derived Exosomes
of Pulmonary and Extra-Pulmonary Tuberculosis Patients as Compared
to HIV-Tuberculosis Dual-infected Patients and Healthy Humans
Authors: Vir  Singh Ajay , Kushwaha Shweta , Yadav Rajbala , Davuluri Kusuma Sai , Goel Anjana , Chauhan Devendra Singh
Published: May 2023

Background:

Despite the crucial involvement of vitronectin in affecting the perseverance
of certain respiratory pathogens and the progression of several lung diseases, the association of vitronectin
with tuberculosis (TB) has been poorly studied. The present study aimed to determine whether
vitronectin levels are altered in TB patients compared to healthy humans.

Methods:

Twenty-four laboratory-confirmed tuberculosis patients (pulmonary TB -8, extrapulmonary-
8 and HIV-TB dual infected -8) and eight healthy individuals were included in this study.
The quantitative detection of vitronectin in serum-derived exosomes of study participants was performed
using a sandwich enzyme-linked immunosorbent assay. Measured concentrations of vitronectin
were compared with the demographic variables of the study participants and between the study
groups. The Mann–Whitney U unpaired test was used in statistical analysis, and the p-value < 0.05
was considered statistically significant.

Results:

Vitronectin was detected in serum-derived exosomes of all study participants. The demographic
characteristics (gender, age, smoking and alcohol consumption habit, history of cough, and
weight loss) were not significantly correlated with the vitronectin concentrations of the study participants
(p-value> 0.05). The level of vitronectin was higher in patients with pulmonary TB (778.54 ng/l)
and extra-pulmonary-TB patients (773.04 ng/l) while lower in HIV-pulmonary TB dual-infected patients
(354.86 ng/l) as compared to healthy humans (456.20ng/l). There was a significant difference
between vitronectin concentrations of patients with pulmonary TB (p-value: 0.0002) and extrapulmonary
TB (p-value: 0.003) compared to healthy controls.

Conclusion:

The present study reported an increased concentration of vitronectin in serum-derived
exosomes of pulmonary and extra-pulmonary TB patients compared to HIV-TB dual-infected patients
and healthy humans. Further studies are needed to fully elucidate the diagnostic potential and functionalities
of higher concentrations of vitronectin in the pathogenic processes of human TB.
18 F-FDG PET/MRI of Primary Hepatic Malignancies: Differential Diagnosis
and Histologic Grading
Authors: Sökmen Bedriye Koyuncu , Inan Nagihan
Published: May 2023

Background:

Distinguishing between IHCC and HCC is important because of their differences in treatment and prognosis. The hybrid positron emission Tomography/magnetic Resonance Imaging (PET/MRI) system has become more widely accessible, with oncological imaging becoming one of its most promising applications

Objective:

The objective of this study was to see how well 18F-fluorodeoxyglucose (18F-FDG) PET/MRI could be used for differential diagnosis and histologic grading of primary hepatic malignancies.

Methods:

We retrospectively evaluated 64 patients (53 with HCC, 11 with IHCC) with histologically proven primary hepatic malignancies using 18F-FDG/MRI. The apparent diffusion coefficient (ADC), coefficient of variance (cv) of the ADC, and standardized uptake value (SUV) were calculated.

Results:

The mean SUVmax value was higher for IHCC (7.7 ± 3.4) than for HCC (5.2 ± 3.1) (p = 0.019). The area under the curve (AUC) was 0.737, an optimal 6.98 cut-off value providing 72% sensitivity and 79% specificity. The ADCcv value in IHCC was statistically significantly higher than in HCC (p=0.014). ADC mean values in HCCs were significantly higher in low-grade tumors than in high-grade tumors. The AUC value was 0.73, and the optimal cut-off point was 1.20x10-6 mm2/s, giving 62% sensitivity and 72% specificity. The SUVmax value was also found to be statistically significantly higher in the high-grade group. The ADCcv value in the HCC low-grade group was found to be lower than in the high-grade group (p=0.036).

Conclusion:

18F FDG PET/MRI is a novel imaging technique that can aid in the differentiation of primary hepatic neoplasms as well as tumor-grade estimation.
Effect of Lactobacillus rhamnosus and Diclofenac with Curcumin for Neuronal
Restoration and Repair Against Scopolamine Induced Dementia in
Zebrafish (Danio rerio)
Authors: Pande Sonal , Patel Chirag
Published: May 2023

Background:

Clinical studies have already revealed the ubiquitous neuroprotective role of
curcumin in neuronal deterioration, but it cannot be used alone due to its truncated bioavailability. Currently,
many such approaches are functional, which overcome this issue either by increasing the solubility
or absorption. These approaches carry a costlier treatment. One more tactic is present but less focused
i.e., by limiting the intestine and liver enzymatic metabolism; by this approach, curcumin will be
more available for its beneficial outcome.

Objective:

The goal of this study was to evaluate the impact of Lactobacillus rhamnosus and diclofenac
on the neuroprotective effects of curcumin against scopolamine-induced dementia.

Methods:

Physical parameters involved a novel tank test, T maze test, whereas neurochemical parameters
include brain oxidative stress and acetylcholinesterase (Ache) inhibition activity in a zebrafish dementia
model.

Results:

Our results demonstrated that curcumin with Lactobacillus rhamnosus and diclofenac significantly
(p<0.05) reduced anxiety, memory deficits, and brain oxidative stress compared to the alone curcumin-
treated group.

Conclusion:

This result approves that curcumin with L.rhamnosus and diclofenac have superior activity
compared to curcumin alone. However, further clinical studies are needed to validate these findings.
Case Report: Novel Pathogenic Variant Detected in Two Siblings with
Type 1 Gaucher Disease
Authors: Dursun Huseyin , Metli Kubra , Bayram Fahri
Published: May 2023

Background:

Gaucher disease (GD) is an autosomal recessive lysosomal storage disease. The disease develops due to glucocerebrosidase enzyme deficiency caused by biallelic pathogenic variants in the glucosylceramidase beta 1 (GBA1) gene, which encodes the glucocerebrosidase en-zyme. The GBA1 gene is located at chromosomal location 1q22 and consists of 11 exons. In this article, we report a novel pathogenic variant in the GBA1 gene.

Case Presentations:

A 32-year-old female patient with no known chronic disease was admitted with complaints of weakness, bone pain, and abdominal pain. Her evaluation included hepatosplenomeg-aly, thrombocytopenia, osteoporosis, and anemia. The clinical suspicion of Gaucher disease was confirmed by glucocerebrosidase enzyme level and genetic testing. In her family screening, her sister also had hepato-splenomegaly, osteoporosis, thrombocytopenia, and anemia. Both sisters had no neurological symptoms. As a result of GBA1 gene sequence analysis in two of our patients, a missense variant was detected in the c.593C > A homozygous genotype. This variant has not been reported in any previously published case.

Conclusion:

In this case report, we aimed to contribute to the literature by reporting a new novel pathogenic variant in the GBA1 gene leading to type 1 Gaucher disease that has not been described before.
Research on Visual Inspection Method of Tree Whitening Quality based
on Multi-level Feature Fusion
Authors: Cao Yangfan , Wang Wenchao , Zhao Ying , Sun Qun
Published: May 2023

Background:

At present, the traditional manual whitewashing method is used in tree whitewashing in China. The quality of tree whitening can only be judged by the naked eye.

Objective:

Up to now, the quality of tree whitewashing is still judged manually. In order to improve work efficiency, an automatic evaluation method of tree whitewashing quality based on multi-level feature fusion is proposed.

Methods:

The images extract texture features from white-washed trees (gray-level co-occurrence matrix) and shape features (gradient direction histogram) from pixel-level fusion to obtain a global characteristic matrix. Using a support vector machine (SVM), random forests, and clustering algorithm (KNN), three classifiers were selected to identify the integration characteristics of the training test. In order to reduce the correlation of feature information and improve the execution efficiency of the classifier, the global feature matrix was optimized by combining principal component analysis (PCA) with pixel-level fusion and feature-level fusion.

Results:

The experiment results showed that the classification accuracy of the support vector machine is 94.00%, which is higher than that of the random forest classifier (92.67) and KNN classifier (92.67%). Meanwhile, the support vector machine is superior to the other two classification algorithms in recall rate, accuracy rate, and algorithm execution efficiency. The results showed that the execution efficiency of each classification algorithm was improved after the optimization of the feature data. The support vector machine classification algorithm is more stable than the other two algorithms.

Conclusion:

The feature fusion method combined with PCA can improve the execution efficiency and recognition precision of classifiers to a certain extent. For the feature matrix obtained by different data processing methods, the SVM classifier performs more stably and reliably than the random forest classifier and KNN in the inspection of tree whitening quality.
pH-metric titration technique for the study of metal-complexes of substituted 2- Oxo-2H-Chromene-3-Carbohydrazide derivatives in solution: calculation of stability constants
Authors: Choudhari P. P. , Dongapure A. C.
Published: May 2023

Background:

In coordination chemistry, the stability of complexes is expressed in terms of the formation constant of complexes. In coordination complexes, the temperature is very important. It plays an important role in complex formation reaction. Temperature affects metal-ligand stability constant (log K) and proton-ligand stability constant (pK). The metal ion Mn2+, Co2+, Ni2+, and Cu2+ at different temperatures form complexes with 4-sulfamethoxazoleazo-3-methyl-2-pyrazolin-5-one are reported. The metal-ligand stability constant (logK) and proton-ligand stability constant (pK) of Mn(II), Co(II), Ni(II), Cu(II), and Zn(II) with organic ligand n-[2-hydroxy-1-napthalydene]-2-methylanilline at 0.1 M ionic strength in 60% dioxane-water medium is studied. The study of thermodynamic parameters and stability constant of complexes of substituted thiazole Schiff bases with rare earth metal ions in mixed solvent is reported.

Objective:

The study of coordination compounds and a lot of work has been done on metal-ligand stability constant. The same transition metal ion form complexes with substituted pyrazole for this metal-ligand stability constant are determined.

Method:

Pointwise Calculation Method Half Integral Method.

Result:

The values of logK1 and logK2 are determined from the metal-ligand formation curve at formation numbers 0.5 and 1.5. In all cases, logK1 is greater than logK2. The ratio logK1/logK2 is positive and greater than one in all systems. This implies that there is little or no steric hindrance to the addition of the secondary ligand molecules. The difference between logK1 - logK2 is usually positive. If the difference between logK1 and logK2 is less than 2.5, simultaneous formation of 1:1 and 1:2 complexes occurs and if it is more than 2.5, then stepwise complex formation occurs. In the present case, in all systems, it is less than 2.5. This indicates the simultaneous formation of 1:1 and 1:2 complexes takes place.

Conclusion:

A pH-metric study of Substituted 2-oxo-2H-chromene-3-carbohydrazide derivatives with metal ions Fe(III), and Mn(II) occurs. The graphical representation of the curve shows that the acid + ligand curve is separated from the corresponding acid + ligand + metal curve in this system. The formation of a complex is indicated. Half integral method and pointwise calculation method give nearly similar results. The values of stability constants for all systems indicate that the complexes formed in these processes are stable.