bluuebunny/crossref_metadata_2025 huggingface dataset:
Plasma Catestatin Levels and Advanced Glycation End Products in Patients on Hemodialysis
Authors: Luketin Mirko , Mizdrak Maja , Boric-Skaro Dijana , Martinovic Dinko , Tokic Daria , Vilovic Marino , Supe-Domic Daniela , Ticinovic Kurir Tina , Bozic Josko
Published: March 2021

Catestatin (CST) is a pleiotropic peptide involved in cardiovascular protection with its antihypertensive and angiogenic effects. Considering that patients with end-stage renal disease (ESRD) who are undergoing hemodialysis (HD) are associated with higher cardiovascular risk, the aim of this study was to investigate plasma CST levels in HD patients, compare them to healthy controls and evaluate possible CST associations with advanced glycation end products (AGEs) and laboratory, anthropometric and clinical parameters. The study included 91 patients on HD and 70 healthy controls. Plasma CST levels were determined by an enzyme-linked immunosorbent assay in a commercially available diagnostic kit, while AGEs were determined using skin autofluorescence. Plasma CST levels were significantly higher in the HD group compared to the controls (32.85 ± 20.18 vs. 5.39 ± 1.24 ng/mL, p < 0.001) and there was a significant positive correlation between CST and AGEs (r = 0.492, p < 0.001). Furthermore, there was a significant positive correlation between plasma CST levels with both the Dialysis Malnutrition Score (r = 0.295, p = 0.004) and Malnutrition-Inflammation Score (r = 0.290, p = 0.005). These results suggest that CST could be playing a role in the complex pathophysiology of ESRD/HD and that it could affect the higher cardiovascular risk of patients on HD.
Operational Modal Analysis, Model Update and Fragility Curves Estimation, through Truncated Incremental Dynamic Analysis, of a Masonry Belfry
Authors: Capanna Ilaria , Cirella Riccardo , Aloisio Angelo , Alaggio Rocco , Di Fabio Franco , Fragiacomo Massimo
Published: March 2021

Masonry towers, located in seismic zones, are vulnerable and prone to damages up to compromise their stability. The scatter of data on the mechanical properties of masonry, geometry and boundary conditions determine a lack of building knowledge on their expected behaviour. Therefore the assessment of the seismic capacity represents a critical task. This paper contributes to the issue of seismic analysis of masonry towers, focusing a meaningful case study: the St.Silvestro belfry in L’Aquila, Italy. The tower, severely damaged by the 2009 earthquake sequence, underwent extensive restoration works, endeavoured to mitigate its vulnerability. The observed seismic damage, the performed no-destructive testing campaign and the accomplished rehabilitation measures are described in the paper. The authors appraised the actual seismic performances of the St.Silvestro belfry, reinforced by the last restoration works. At first, the Operational Modal Analysis (OMA) is carried out to enhance building knowledge. In a second step, a refined finite element model is calibrated on the results from OMA to seize the actual dynamic response. Ultimately, by using the updated finite element model, the authors estimate the fragility curves in terms of peak ground acceleration using truncated incremental dynamic analyses.
Identification of Environmental and Contextual Driving Factors of Air Conditioning Usage Behaviour in the Sydney Residential Buildings
Authors: Jeong Bongchan , Kim Jungsoo , Ma Zhenjun , Cooper Paul , de Dear Richard
Published: March 2021

Air conditioning (A/C) is generally responsible for a significant proportion of total building energy consumption. However, occupants’ air conditioning usage patterns are often unrealistically characterised in building energy performance simulation tools, which leads to a gap between simulated and actual energy use. The objective of this study was to develop a stochastic model for predicting occupant behaviour relating to A/C cooling and heating in residential buildings located in the Subtropical Sydney region of Australia. Multivariate logistic regression was used to estimate the probability of using A/C in living rooms and bedrooms, based on a range of physical environmental (outdoor and indoor) and contextual (season, day of week, and time of day) factors observed in 42 Sydney region houses across a two-year monitoring period. The resulting models can be implemented in building energy performance simulation (BEPS) tools to more accurately predict indoor environmental conditions and energy consumption attributable to A/C operation.
Identification of an Immature Subset of PMN-MDSC Correlated to Response to Checkpoint Inhibitor Therapy in Patients with Metastatic Melanoma
Authors: Gondois-Rey Françoise , Paul Magali , Alcaraz Florence , Bourass Sarah , Monnier Jilliana , Malissen Nausicaa , Grob Jean-Jacques , Bruger Annika M. , Van Der Bruggen Pierre , Gaudy-Marqueste Caroline , Olive Daniel
Published: March 2021

PMN-MDSCs support tumor progression and resistance to ICI therapy through their suppressive functions but their heterogeneity limits their use as biomarkers in cancer. Our aim was to investigate the phenotypic and functional subsets of PMN-MDSCs to identify biomarkers of response to ICI therapy. We isolated low-density CD15+ PMNs from patients with metastatic melanoma and assessed their immune-suppressive capacities. Expression of CD10 and CD16 was used to identify mature and immature subsets and correlate them to inhibition of T cell proliferation or direct cytotoxicity. Frequencies of the PMN-MDSCs subsets were next correlated to the radiological response of 36 patients receiving ICI therapy. Mature activated cells constituted the major population of PMN-MDSCs. They were found in a higher proportion in the pre-treatment blood of patients non responders to ICI. A subset of immature cells characterized by intermediate levels of CD10 and CD16, the absence of expression of SIRPα and a strong direct cytotoxicity to T cells was increased in patients responding to ICI. The paradoxical expansion of such cells during ICI therapy suggests a role of PMNs in the inflammatory events associated to efficient ICI therapy and the usefulness of their monitoring in patients care.
Salvage Radiation for Pelvic Relapse after Surgically Treated Endometrial Cancer
Authors: Lindemann Kristina , Smogeli Elisabeth , Småstuen Milada Cvancarova , Bruheim Kjersti , Trovik Jone , Nordberg Terje , Kristensen Gunnar B. , Werner Henrica M. J. , Nakken Esten
Published: March 2021

(1) Background: This study evaluated the clinical outcome after salvage radiotherapy for first pelvic relapse after endometrial cancer (EC). (2) Methods: This multicenter retrospective study included EC patients with first central pelvic relapse without lymph node involvement treated with curative intent. Progression-free (PFS) and overall survival (OS) were calculated with the Kaplan–Meier method and possible predictive factors for risk of relapse and mortality were identified using the Cox model. (3) Results: We included 139 patients with median EQD2 (Equivalent Dose in 2 Gy fractions) to the clinical target volume of 70.0 Gy. During follow up of median 6.66 years, 39.6% patients developed a second relapse. Risk group classification at primary diagnosis based on histology, grading and FIGO stage and how the pelvic tumor boost was administered were independently associated with PFS and OS. Five-year OS was 68% (95% CI (59–75)) for the whole cohort. Five-year OS was 88% (95% CI (75–94)), 72% (95% CI (55–84)) and 38% (95% CI (15–60)) for the stage I low-, intermediate- and high-risk group, respectively. (4) Conclusions: The majority of central pelvic recurrences in RT-naive EC women can be successfully salvaged with radiotherapy. However, survival in patients with high-risk disease remains poor and warrants a more individualized approach to optimize outcome.
The MEK1/2 Pathway as a Therapeutic Target in High-Grade Serous Ovarian Carcinoma
Authors: Chesnokov Mikhail S. , Khan Imran , Park Yeonjung , Ezell Jessica , Mehta Geeta , Yousif Abdelrahman , Hong Linda J. , Buckanovich Ronald J. , Takahashi Akimasa , Chefetz Ilana
Published: March 2021

High-grade serous ovarian carcinoma (HGSOC) is the deadliest of gynecological cancers due to its high recurrence rate and acquired chemoresistance. RAS/MEK/ERK pathway activation is linked to cell proliferation and therapeutic resistance, but the role of MEK1/2-ERK1/2 pathway in HGSOC is poorly investigated. We evaluated MEK1/2 pathway activity in clinical HGSOC samples and ovarian cancer cell lines using immunohistochemistry, immunoblotting, and RT-qPCR. HGSOC cell lines were used to assess immediate and lasting effects of MEK1/2 inhibition with trametinib in vitro. Trametinib effect on tumor growth in vivo was investigated using mouse xenografts. MEK1/2 pathway is hyperactivated in HGSOC and is further stimulated by cisplatin treatment. Trametinib treatment causes cell cycle arrest in G1/0-phase and reduces tumor growth rate in vivo but does not induce cell death or reduce fraction of CD133+ stem-like cells, while increasing expression of stemness-associated genes instead. Transient trametinib treatment causes long-term increase in a subpopulation of cells with high aldehyde dehydrogenase (ALDH)1 activity that can survive and grow in non-adherent conditions. We conclude that MEK1/2 inhibition may be a promising approach to suppress ovarian cancer growth as a maintenance therapy. Promotion of stem-like properties upon MEK1/2 inhibition suggests a possible mechanism of resistance, so a combination with CSC-targeting drugs should be considered.
Genetic and Non-Genetic Mechanisms Underlying Cancer Evolution
Authors: Shlyakhtina Yelyzaveta , Moran Katherine L. , Portal Maximiliano M.
Published: March 2021

Cancer development can be defined as a process of cellular and tissular microevolution ultimately leading to malignancy. Strikingly, though this concept has prevailed in the field for more than a century, the precise mechanisms underlying evolutionary processes occurring within tumours remain largely uncharacterized and rather cryptic. Nevertheless, although our current knowledge is fragmentary, data collected to date suggest that most tumours display features compatible with a diverse array of evolutionary paths, suggesting that most of the existing macro-evolutionary models find their avatar in cancer biology. Herein, we discuss an up-to-date view of the fundamental genetic and non-genetic mechanisms underlying tumour evolution with the aim of concurring into an integrated view of the evolutionary forces at play throughout the emergence and progression of the disease and into the acquisition of resistance to diverse therapeutic paradigms. Our ultimate goal is to delve into the intricacies of genetic and non-genetic networks underlying tumour evolution to build a framework where both core concepts are considered non-negligible and equally fundamental.
Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments
Authors: Oliveira Micaela , Conceição Pedro , Kant Krishna , Ainla Alar , Diéguez Lorena
Published: March 2021

Currently, conventional pre-clinical in vitro studies are primarily based on two-dimensional (2D) cell culture models, which are usually limited in mimicking the real three-dimensional (3D) physiological conditions, cell heterogeneity, cell to cell interaction, and extracellular matrix (ECM) present in living tissues. Traditionally, animal models are used to mimic the 3D environment of tissues and organs, but they suffer from high costs, are time consuming, bring up ethical concerns, and still present many differences when compared to the human body. The applications of microfluidic-based 3D cell culture models are advantageous and useful as they include 3D multicellular model systems (MCMS). These models have demonstrated potential to simulate the in vivo 3D microenvironment with relatively low cost and high throughput. The incorporation of monitoring capabilities in the MCMS has also been explored to evaluate in real time biophysical and chemical parameters of the system, for example temperature, oxygen, pH, and metabolites. Electrochemical sensing is considered as one of the most sensitive and commercially adapted technologies for bio-sensing applications. Amalgamation of electrochemical biosensing with cell culture in microfluidic devices with improved sensitivity and performance are the future of 3D systems. Particularly in cancer, such models with integrated sensing capabilities can be crucial to assess the multiple parameters involved in tumour formation, proliferation, and invasion. In this review, we are focusing on existing 3D cell culture systems with integrated electrochemical sensing for potential applications in cancer models to advance diagnosis and treatment. We discuss their design, sensing principle, and application in the biomedical area to understand the potential relevance of miniaturized electrochemical hybrid systems for the next generation of diagnostic platforms for precision medicine.
Risk Stratification for Immediate Postoperative Hearing Loss by Preoperative BAER (Brainstem Auditory Evoked Response) and Audiometry in NF2-Associated Vestibular Schwannomas
Authors: Gugel Isabel , Grimm Florian , Hartjen Philip , Breun Maria , Zipfel Julian , Liebsch Marina , Löwenheim Hubert , Ernemann Ulrike , Kluwe Lan , Mautner Victor-Felix , Tatagiba Marcos , Schuhmann Martin Ulrich
Published: March 2021

Both brainstem auditory evoked potentials (BAEP) and audiometry play a crucial role in neuro-oncological treatment decisions in Neurofibromatosis Type 2 associated (NF2) vestibular schwannoma (VS) as hearing preservation is the major goal. In this study, we investigated the risk of immediate postoperative hearing deterioration (>15 dB and/or 15% loss in pure-tone average [PTA]/ speech discrimination score [SDS] in a cohort of 100 operated VS (ears) in 72 NF2 patients by retrospective analysis of pre- and postoperative hearing data (PTA, SDS, American Association of Otolaryngology–Head and Neck Surgery [AAO-HNS], and brainstem auditory evoked potential [BAEP] class) taking into account relevant influencing factors, particularly preoperative audiometry and BAEP status and the extent of resection. Immediately after surgery, the hearing was preserved in 73% of ears and approximately ~60% of ears kept their hearing classes. Preoperative BAEP (p = 0.015) and resection amount (p = 0.048) significantly influenced postoperative hearing outcome. The prediction model for postoperative hearing deterioration/loss between preoperative BAEP and AAO-HNS class showed increased risk by increasing BAEP class. Twenty-one tumors/ears were identified with large BAEP and AAO-HNS class discrepancies (≥2 points) and were associated with a high (48–100%) risk of deafness after surgery in ears with preoperative available hearing. Overall, the results were heterogeneous but the better both BAEP and audiometry class before surgery, the higher the chance of hearing maintenance afterwards. Large resection amounts (e.g., 100% risk in near-total resections) exhibit a significant (p < 0.05) higher risk compared to smaller amounts (e.g., 10/20% in laser-coagulated/partially resected tumors). Our results emphasized the indispensable role of both hearing monitoring in form of audiometry and neurophysiology (BAEP) in the pre-and perioperative monitoring of NF2-associated VS. Both BAEP and audiometry are good prognostic markers for the postoperative hearing outcome. The extent of resection should be strictly guided by and adjusted to the intraoperative neurophysiological monitoring.
Colour Changes during the Carbamazepine Oxidation by Photo-Fenton
Authors: Villota Natalia , Ferreiro Cristian , Qulatein Hussein Ahmad , Lomas Jose María , Camarero Luis Miguel , Lombraña José Ignacio
Published: March 2021

The oxidation of aqueous solutions of carbamazepine is conducted using the Fenton reagent, combined with the photolytic action of a 150 W medium pressure UV lamp, operating at T = 40 °C. The effect of acidity is analysed at an interval pH = 2.0–5.0, verifying that operating at pH = 5.0 promotes colour formation (Colour = 0.15 AU). The effect of iron is studied, finding that the colour of the water increases in a linear way, Colour = 0.05 + 0.0075 [Fe]0. The oxidising action of hydrogen peroxide is tested, confirming that when operating with [H2O2]0 = 2.0 mM, the maximum colour is generated (Colourmax = 0.381 AU). The tint would be generated by the degradation of by-products of carbamazepine, which have chromophoric groups in their internal structure, such as oxo and dioxocarbazepines, which would produce tint along the first minutes of oxidation, while the formation of acridones would slowly induce colour in the water.
Acyl–Acyl Carrier Protein Desaturases and Plant Biotic Interactions
Authors: Kazaz Sami , Miray Romane , Baud Sébastien
Published: March 2021

Interactions between land plants and other organisms such as pathogens, pollinators, or symbionts usually involve a variety of specialized effectors participating in complex cross-talks between organisms. Fatty acids and their lipid derivatives play important roles in these biological interactions. While the transcriptional regulation of genes encoding acyl–acyl carrier protein (ACP) desaturases appears to be largely responsive to biotic stress, the different monounsaturated fatty acids produced by these enzymes were shown to take active part in plant biotic interactions and were assigned with specific functions intrinsically linked to the position of the carbon–carbon double bond within their acyl chain. For example, oleic acid, an omega-9 monounsaturated fatty acid produced by Δ9-stearoyl–ACP desaturases, participates in signal transduction pathways affecting plant immunity against pathogen infection. Myristoleic acid, an omega-5 monounsaturated fatty acid produced by Δ9-myristoyl–ACP desaturases, serves as a precursor for the biosynthesis of omega-5 anacardic acids that are active biocides against pests. Finally, different types of monounsaturated fatty acids synthesized in the labellum of orchids are used for the production of a variety of alkenes participating in the chemistry of sexual deception, hence favoring plant pollination by hymenopterans.
The Role of Myeloid-Derived Suppressor Cells (MDSCs) in the Development and/or Progression of Endometriosis-State of the Art
Authors: Suszczyk Dorota , Skiba Wiktoria , Jakubowicz-Gil Joanna , Kotarski Jan , Wertel Iwona
Published: March 2021

Endometriosis (EMS) is a common gynecological disease characterized by the presence of endometrial tissue outside the uterus. Approximately 10% of women around the world suffer from this disease. Recent studies suggest that endometriosis has potential to transform into endometriosis-associated ovarian cancer (EAOC). Endometriosis is connected with chronic inflammation and changes in the phenotype, activity, and function of immune cells. The underlying mechanisms include quantitative and functional disturbances of neutrophils, monocytes/macrophages (MO/MA), natural killer cells (NK), and T cells. A few reports have shown that immunosuppressive cells such as regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) may promote the progression of endometriosis. MDSCs are a heterogeneous population of immature myeloid cells (dendritic cells, granulocytes, and MO/MA precursors), which play an important role in the development of immunological diseases such as chronic inflammation and cancer. The presence of MDSCs in pathological conditions correlates with immunosuppression, angiogenesis, or release of growth factors and cytokines, which promote progression of these diseases. In this paper, we review the impact of MDSCs on different populations of immune cells, focusing on their immunosuppressive role in the immune system, which may be related with the pathogenesis and/or progression of endometriosis and its transformation into ovarian cancer.
Experimental Analysis of Gas Holdup Measured by Gas Array Tool in Gas–Water Two Phase of Horizontal Well
Authors: Cui Shuaifei , Liu Junfeng , Chen Xulong , Li Qinze
Published: March 2021

In the gas-water two phase of horizontal well, gas holdup is usually obtained by inverse calculation of the water holdup measured by the array capacitance probes. Gas Array Tool (GAT) has been developed to directly measure gas holdup. This instrument has been introduced into China and its simulation experiment in gas-water two phase flow in horizontal wells has been carried out for the first time to study the applicability of gas holdup measurement. Firstly, the response principle and measurement method of GAT are analyzed; secondly, the experimental data of GAT under different flowrates, water cut, and different cable speed are plotted and analyzed; finally, the gas holdup data measured by GAT and Capacitance Array Tool (CAT) are compared by using an interpolation algorithm. It is found that the response of the optical fiber probe is consistent and stable. It also proves the accuracy of gas identification and the applicability of gas holdup measurement under test conditions by GAT, which lays a foundation for further gas holdup measurement, interpretation, and field test in the future.
Shoulder Implant Manufacturer Detection by Using Deep Learning: Proposed Channel Selection Layer
Authors: Yılmaz Atınç
Published: March 2021

Total Shoulder Arthroplasty (TSA) is the process of replacing the damaged ball and socket joint in the shoulder with a prosthesis made with polyethylene and metal components. After this procedure, intervention may be required as a result of damage to the prosthesis, except for the need for an examination regarding the prosthesis at certain periods. If the patient does not have information about the model and manufacturer of the prosthesis, the treatment process is delayed. Artificial intelligence-assisted systems can speed up the treatment process by classifying the manufacturer and model of the prosthesis. In this study, artificial intelligence methods were applied to classify shoulder implants using X-ray images. The model and manufacturer of the prosthesis is detected by using the proposed deep learning method. Besides, the most commonly used machine learning classifiers were applied for the same problem to compare the results and show the effectiveness of the proposed method. In addition, the accuracy and precision analysis and measurements of the processing times for the applied methods were performed to reveal the performance, accuracy, and efficiency of the study. In order to measure the performance of the proposed method, it was compared with studies on the same problem in the literature. As a result of the comparison, it was found that the proposed method, with an accuracy rate of 97.2%, performed better than the other studies. In the study, the implant manufacturer and model are classified in order to carry out the implant surgery process in the best way with the proposed deep learning model. With the success of the proposed system, the applicability of this model in similar prosthesis classifications has been shown. Differently from the studies in the literature, the channel selection formula is presented in the proposed deep learning method recommended for selecting the most distinctive feature filters.
Polymer-Cement Composites Glazing by Concentrated Solar Energy
Authors: Baltes Liana , Patachia Silvia , Ekincioglu Ozgur , Ozkul Hulusi , Croitoru Catalin , Munteanu Corneliu , Istrate Bogdan , Tierean Mircea
Published: March 2021

Macro defect free (MDF) cements are polymer-cement composites characterized by high biaxial flexural strength compared to traditional concrete, having as a drawback a low water resistance. Glazing these composite materials with an inorganic enamel containing TiO2 nano-particles has led to a high water-stable material with advanced photocatalytic properties. Classic glazing by thermal treatment of samples, at 1050 °C, requires energy consumption and long-time performing. The purpose of this paper is to test the use of solar radiation as a source of energy in the glazing process. A vertical axis solar furnace has been used, from PROMES-CNRS Solar Laboratory, Font-Romeu Odeillo, France, and it has been observed that a uniform appearance of the glaze coating has been achieved; it shows high scratch resistance, meaning a good hardness and adhesion to the substrate. The obtained film was also characterized by SEM, EDS and XRD, aiming to evidence the coat morphology, the TiO2 distribution and its crystallinity alteration, when compared to the samples obtained by classic thermal treatment. The conclusion of the paper is that using solar radiation in the MDF cement glazing process is a promising approach for obtaining multifunctional materials.
Temporomandibular Joint Osseous Morphology of Class I and Class II Malocclusions in the Normal Skeletal Pattern: A Cone-Beam Computed Tomography Study
Authors: Fan Xiao-Chuan , Ma Lin-Sha , Chen Li , Singh Diwakar , Rausch-Fan Xiaohui , Huang Xiao-Feng
Published: March 2021

(1) Background—The aim of the present study was to evaluate the correlation between the temporomandibular joint (TMJ) osseous morphology of normal skeletal pattern individuals with different dental malocclusions by using cone-beam computed tomography (CBCT). (2) Methods—The CBCT images of bilateral TMJs in 67 subjects with skeletal class I and average mandibular angle (26 males and 41 females, age range 20–49 years) were evaluated in this study. The subjects were divided into class I, class II division 1, and class II division 2 according to the molar relationship and retroclination of the maxillary incisors. Angular and linear measurements of TMJ were evaluated and the differences between the groups were statistically analyzed. (3) Results—Intragroup comparisons showed statistical differences for articular eminence inclination, the width of the glenoid fossa, the ratio of the width of the glenoid fossa to the depth of the glenoid fossa, the condylar angle, and the intercondylar angle between the malocclusion groups. The measurements of the glenoid fossa shape showed no significant difference between the left and right sides. Females showed more differences in the morphological parameters of TMJ between the three malocclusion groups than the males. (4) Conclusion—The present study revealed differences in the TMJ osseous morphology between dental class I and class II malocclusions in the normal skeletal pattern.
The Entire Intestinal Tract Surveillance Using Capsule Endoscopy after Immune Checkpoint Inhibitor Administration: A Prospective Observational Study
Authors: Shimozaki Keitaro , Hirata Kenro , Horie Sara , Chida Akihiko , Tsugaru Kai , Hayashi Yukie , Kawasaki Kenta , Miyanaga Ryoichi , Hayashi Hideyuki , Mizuno Ryuichi , Funakoshi Takeru , Hosoe Naoki , Hamamoto Yasuo , Kanai Takanori
Published: March 2021

Background: Despite the proven efficacy of immune checkpoint inhibitors (ICIs) against various types of malignancies, they have been found to induce immune-related adverse events, such as enterocolitis; however, the clinical features of ICI-induced enterocolitis remain to be sufficiently elucidated, which is significant, considering the importance of early detection in the appropriate management and treatment of ICI-induced enterocolitis. Therefore, the current study aimed to determine the utility of capsule endoscopy as a screening tool for ICI-induced enterocolitis. Methods: This single-center, prospective, observational study was conducted on patients with malignancy who received any ICI between April 2016 and July 2020 at Keio University Hospital. Next, second-generation capsule endoscopy (CCE-2) was performed on day 60 after ICI initiation to explore the entire gastrointestinal tract. Results: Among the 30 patients enrolled herein, 23 underwent CCE-2. Accordingly, a total of 23 findings were observed in 14 (60.8%) patients at any portion of the gastrointestinal tract (7 patients in the colon, 4 patients in the small intestine, 2 patients in both the colon and the small intestine, and 1 patient in the stomach). After capsule endoscopy, 2 patients (8.7%) developed ICI-induced enterocolitis: both had significantly higher Capsule Scoring of Ulcerative Colitis than those who had not developed ICI-induced enterocolitis (p = 0.0455). No adverse events related to CCE-2 were observed. Conclusions: CCE-2 might be a safe and useful entire intestinal tract screening method for the early detection of ICI-induced enterocolitis in patients with malignancies.
Comparisons of Modeling Methods for Fractional-Order Cuk Converter
Authors: Wang Xiaogang , Qiu Bingwen , Wang Hongdong
Published: March 2021

In this paper, several modeling methods for the continuous current mode (CCM) fractional-order Cuk converter are investigated. First, the state space averaging method is used to establish the model. Based on this model, the expressions of inductors’ current and capacitor voltage as well as the transfer functions are derived. Then, the equivalent small parameter method (ESPM) is employed to model the converter. Based on the Oustaloup filter principle, the approximate models of fractional-order capacitor and inductors are constructed, which consist of integer-order components, to build the circuit model (CM) of the converter. In addition, the numerical model (NM) of the converter is established. Simulation results are provided to compare the modeling methods, which show that the ESPM has some advantages over the other methods. Finally, the hardware-in-the-loop experiment is conducted to verify the effectiveness of the circuit model.
A Multireceiver Wireless Power Supply System with Power Equalization in Stereoscopic Space
Authors: Shi Bin , Wen Feng , Chu Xiaohu
Published: March 2021

With the rapid development of wireless power transfer (WPT) technology, the traditional single-transceiver WPT system has become more and more advanced; however, it is still difficult to meet its extensive application requirements. Aiming at the wireless charging of mobile phones in public places, electric vehicles (EVs) in multistorey garages, and electronic shelf labels (ESLs) in supermarket merchandise shelves, a multireceiver wireless power supply system with power equalization is proposed. The condition of power equalization is derived according to the equivalent circuit of the proposed WPT system, and the received power can be equally maintained by adjusting the transceiver loop resistance when the total load number or transmission distance changes. A simulation model is established to evaluate the electromagnetic environment of the proposed WPT system, and the results comply with the electromagnetic safety of the ICNIRP-2018 guidelines. Finally, the experimental results show that the power differential rate that meets the power equalization condition is 13 to 17% lower than that of the unsatisfied rate, which verifies the effectiveness of the proposed system in terms of power equalization.
Agroforestry Systems and Their Contribution to Supplying Forest Products to Communities in the Chure Range, Central Nepal
Authors: Khadka Deepa , Aryal Anisha , Bhatta Kishor Prasad , Dhakal Bed Prakash , Baral Himlal
Published: March 2021

Agroforestry (AF), an integration of agricultural and/or pastureland and trees, is a powerful tool for the maximization of profit from a small unit of land; however, it has been less well explored and recognized by existing policies. AF could be the best approach to conserving the fragile soils of Chure and to supplying subsistence needs to the local people. This study endeavored to understand how the adoption of various AF practices contributed to people’s livelihoods in the Bakaiya rural municipality of Makawanpur District. To achieve this, 5 focus group discussions, 10 key informant interviews and 100 household surveys were conducted. These were analyzed using various statistical analysis tools: Kruskal–Wallis test, Games–Howell post hoc comparison test and Wilcoxon test. Thematic analysis was employed to understand the status and growth process of AF in the study area. Of three different AF systems used in the area, agri-silviculture was found to be the dominant form. Local people derived forest products, especially fuelwood, fodder and leaf litter from AF, where agri-silvi-pasture was most common. The three AF systems studied here were in turn compared with community forestry (CF), which is a participatory forest management system overseen by the community. People derived almost 75% of fuelwood from CF, whereas in the case of fodder and leaf litter, contributions from CF and AF were almost equal. Despite the potentiality of AF in fulfilling the demands of local people, promotional and development activities were lacking. This study recommends a strong collaboration of local people and concerned stakeholders for the promotion and technical facilitation of AF systems.
Exploring Best Practices in Geoscience Education: Adapting a Video/Animation on Continental Rifting for Upper-Division Students to a Lower-Division Audience
Authors: Willis Siloa , Stern Robert J. , Ryan Jeffrey , Bebeau Christy
Published: March 2021

Well-crafted and scientifically accurate videos and animations can be effective ways to teach dynamic Earth processes such as continental rifting, both in live course offerings as well as in online settings. However, a quick search of the internet reveals too few high-quality videos/animations describing deep Earth processes. We have modified a hybrid 10.5 min video/animation about continental rifting and the formation of new oceans and passive continental margins created for an upper-division geology audience, retailoring it for a lower-division geology audience. A key challenge in successfully modifying such resources is aligning the cognitive load that the video/animation imposes on students, in part related to the technical geoscientific jargon used in explaining such phenomena, with that which they encounter on these topics in their textbooks and classrooms. We used expert feedback obtained at a 2019 GeoPRISMS (Geodynamic Processes at Rifting and Subducting Margins) workshop in San Antonio to ensure the accuracy of the science content of the upper-division video. We followed this with a review of the terminology and language used in the video/animation, seeking to align the video narrative with the technical language used in introductory geology offerings, which we based on examining five current introductory geology textbooks and feedback from students in introductory geoscience courses. The revised introductory-level video/animation was piloted in an online introductory course, where it provided an improved conceptual understanding of the related processes of continental rifting, opening new oceans, and formation of passive continental margins.
Lifecycle of an Intermontane Plio-Pleistocene Fluvial Valley of the Northern Apennines: From Marine-Driven Incision to Tectonic Segmentation and Infill
Authors: Ghinassi Massimiliano , Aldinucci Mauro , Bianchi Valeria , Brogi Andrea , Capezzuoli Enrico , Yu Tsai-Luen , Shen Chuan-Chou
Published: March 2021

Downcutting and infill of incised valley systems is mostly controlled by relative sea-level changes, and studies on valley-fill successions accumulated independently from relative sea-level or lake-level oscillations are limited. This study focuses on the Plio-Pleistocene evolution of a fluvial drainage system developed in Southern Tuscany (Italy) following a regional marine forced regression at the end of Piacentian. Subsequent in-valley aggradation was not influenced by any relative sea-level rise, and valley morphological and depositional history mainly resulted from interaction between sediment supply and tectonic activity, which caused segmentation of the major valley trunk into localized subsiding depocenters separated by upwarping blocks. Fluvial sedimentation occurred until late Calabrian time, when the major river abandoned that valley, where minor fluvio-lacustrine depocenters allowed accumulation of siliciclastic and carbonate deposits. The present study demonstrates that the infill of the valley was not controlled by the forcing that caused its incision. Accumulation of the fluvial succession is discussed here in relation with localized, tectonic-controlled base levels, which commonly prevent from establishing of a clear downdip stratigraphic correlations. Chronological reconstruction of the study depositional dynamics provides solid constrains to frame them in the tectono-sedimentary evolution of the Northern Apennines.
Paediatric Type1 Diabetes Management and Mothers’ Emotional Intelligence Interactions
Authors: Žilinskienė Jolanta , Šumskas Linas , Antinienė Dalia
Published: March 2021

The functioning of the parents’ emotional sphere is very important to a child’s mental and physical health. This study focused on investigating the association between mothers’ emotional intelligence (EI) and paediatric type I diabetes (T1DM) disease management in their children. We hypothesized that mothers’ EI is associated with T1DM outcomes. Mothers of children with T1DM aged 6–12 years were surveyed. One hundred and thirty-four mothers, the main caregivers of their diabetic children, provided measures of EI and completed a demographic questionnaire. The primary indicator of diabetes management was haemoglobin A1c (HbA1c; the main form of glycosylated haemoglobin). EI scales and subscales were associated with glycaemic management indices. Logistic regression analysis was applied for the assessment of the association between parents’ EI and their paediatric with T1DM disease management. The analysis demonstrated a statistically significant correlation between T1DM management and mothers’ ability to understand and control own emotions, to transform their own negative emotions into positive and to control own negative emotions. Mothers’ EI scales and subscales of understanding and regulating their own emotions, subscales of transforming their own negative emotions into positive ones and controlling their own negative emotions were statistically reliable predictors of glycaemic control in children with T1DM.
Evaluation of Microcirculation, Cytokine Profile, and Local Antioxidant Protection Indices in Periodontal Health, and Stage II, Stage III Periodontitis
Authors: Eldzharov Artem , Kabaloeva Dzerassa , Nemeryuk Dmitry , Goncharenko Aida , Gatsalova Adelina , Ivanova Elena , Kostritskiy Igor , Carrouel Florence , Bourgeois Denis
Published: March 2021

Periodontitis, initiated by the subgingival biofilm and modified by the individual’s inflammatory/immune response, has been associated with vascular dysfunction. To analyze microcirculation indices in periodontal tissues and determine the activity of the enzymatic component of antioxidant defense and humoral immunity factors, a single-blind non-invasive clinical trial was realized. Forty subjects, aged from 30 to 65 years, with moderate to severe chronic periodontitis (chronic generalized periodontitis, CGP) vs. 40 subjects as periodontally healthy were recruited. Information such as capillary diameter, capillary blood flow velocity, concentration of pro- and anti-inflammatory cytokines in serum, vascular endothelial growth factor, and enzymatic component of antioxidant protection were taken. The revealed microcirculatory dysfunctions in patients with CGP clearly demonstrate the progressive disorder of periodontal tissue perfusion and oxygenation, the presence of increased vascular permeability and functional failure of the microvascular system in the lesion. Cytokine profile of CGP patients’ blood serum demonstrated a significant increase of interleukin (IL)-1β, IL-6, tumor necrosis factor α (TNF-α), IL-4 levels as well as statistically significant decrease of IL-1ra, IL-10 concentration. Participants with CGP demonstrated a dominant superiority of IgM and IgG levels. In conclusion, these results contribute to a better understanding of potential correlation between microvascular changes and local and systemic markers of inflammation.
Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis
Authors: Jarecki Jaromir , Małecka-Massalska Teresa , Polkowska Izabela , Potoczniak Bartosz , Kosior-Jarecka Ewa , Szerb Imre , Tomaszewska Ewa , Gutbier Martina , Dobrzyński Maciej , Blicharski Tomasz
Published: March 2021

The aim of this study was to assess levels of adiponectin (ACRP-30) and leptin in serum and synovial fluid (SF) of overweight females with primary gonarthrosis (GOA) and to look for their correlations with clinical status and the level of biochemical OA biomarkers: matrix metalloproteinase (MMP) 1, MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1). The studied group consisted of 39 overweight females undergoing primary total knee arthroplasty as a result of GOA. The stage of GOA was evaluated according to Knee Society Score (KSS), Ahlbäck and Kellgren–Lawrence (K-L) scores. Peripheral blood and SF were obtained. Levels of selected biomarkers were assessed using ELISA kits. The mean level of ACRP-30 in serum valued were 8393.80 ng/mL and in SF, 774.33 ng/mL, the mean concentration of leptin in serum was 32,040.74 pg/mL and in SF, 27,332.12 pg/mL. Levels of leptin in serum and SF correlated with body mass index (BMI), (p = 0.0005, and p = 0.0002, respectively). Levels of ACRP-30 in serum was correlated with clinical scores (Ahlbäck: p = 0.0214; K-L: p = 0.0146). ACRP-30 in SF correlated with ACRP-30 in serum (p = 0.0003), tended to negatively correlate with MMP-1 in serum (p = 0.0598) and positively correlate with pro-MMP-1 in SF (p = 0.0600). To conclude, this study confirms the correlations between concentrations of both, leptin and ACRP-30, comparing serum and SF. In overweight females, leptin levels increase with BMI and ACRP-30 serum level increase in more advanced GOA stages. Finally, leptin levels were correlated with TIMP-1 serum concentration, one of the biochemical markers of GOA.
Body Composition Change, Unhealthy Lifestyles and Steroid Treatment as Predictor of Metabolic Risk in Non-Hodgkin’s Lymphoma Survivors
Authors: Daniele A. , Guarini A. , Summa S. De , Dellino M. , Lerario G. , Ciavarella S. , Ditonno P. , Paradiso A. V. , Divella R. , Casamassima P. , Savino E. , Carbonara M. D. , Minoia C.
Published: March 2021

Unhealthy lifestyle, as sedentary, unbalanced diet, smoking, and body composition change are often observed in non-Hodgkin’s lymphoma (NHL) survivors, and could be determinant for the onset of cancer treatment-induced metabolic syndrome (CTIMetS), including abdominal obesity, sarcopenia, and insulin resistance. The aim of this study was to assess whether changes in body composition, unhealthy lifestyles and types of anti-cancer treatment could increase the risk of metabolic syndrome (MetSyn) and sarcopenia in long-term NHL survivors. We enrolled 60 consecutive NHL patients in continuous remission for at least 3 years. Nutritional status was assessed by anthropometry-plicometry, and a questionnaire concerning lifestyles and eating habits was administered. More than 60% of survivors exhibited weight gain and a change in body composition, with an increased risk of MetSyn. Univariate analysis showed a significantly higher risk of metabolic disorder in patients treated with steroids, and in patients with unhealthy lifestyles. These data suggest that a nutritional intervention, associated with adequate physical activity and a healthier lifestyle, should be indicated early during the follow-up of lymphoma patients, in order to decrease the risk of MetSyn’s onset and correlated diseases in the long term.
Translational Research in the Era of Precision Medicine: Where We Are and Where We Will Go
Authors: De Maria Marchiano Ruggero , Di Sante Gabriele , Piro Geny , Carbone Carmine , Tortora Giampaolo , Boldrini Luca , Pietragalla Antonella , Daniele Gennaro , Tredicine Maria , Cesario Alfredo , Valentini Vincenzo , Gallo Daniela , Babini Gabriele , D’Oria Marika , Scambia Giovanni
Published: March 2021

The advent of Precision Medicine has globally revolutionized the approach of translational research suggesting a patient-centric vision with therapeutic choices driven by the identification of specific predictive biomarkers of response to avoid ineffective therapies and reduce adverse effects. The spread of “multi-omics” analysis and the use of sensors, together with the ability to acquire clinical, behavioral, and environmental information on a large scale, will allow the digitization of the state of health or disease of each person, and the creation of a global health management system capable of generating real-time knowledge and new opportunities for prevention and therapy in the individual person (high-definition medicine). Real world data-based translational applications represent a promising alternative to the traditional evidence-based medicine (EBM) approaches that are based on the use of randomized clinical trials to test the selected hypothesis. Multi-modality data integration is necessary for example in precision oncology where an Avatar interface allows several simulations in order to define the best therapeutic scheme for each cancer patient.
Mitochondrial DNA Analysis Clarifies Taxonomic Status of the Northernmost Snow Sheep (Ovis nivicola) Population
Authors: Dotsev Arsen V. , Kunz Elisabeth , Kharzinova Veronika R. , Okhlopkov Innokentiy M. , Lv Feng-Hua , Li Meng-Hua , Rodionov Andrey N. , Shakhin Alexey V. , Sipko Taras P. , Medvedev Dmitry G. , Gladyr Elena A. , Bagirov Vugar A. , Brem Gottfried , Medugorac Ivica , Zinovieva Natalia A.
Published: March 2021

Currently, the intraspecific taxonomy of snow sheep (Ovis nivicola) is controversial and needs to be specified using DNA molecular genetic markers. In our previous work using whole-genome single nucleotide polymorphism (SNP) analysis, we found that the population inhabiting Kharaulakh Ridge was genetically different from the other populations of Yakut subspecies to which it was usually referred. Here, our study was aimed at the clarification of taxonomic status of Kharaulakh snow sheep using mitochondrial cytochrome b gene. A total of 87 specimens from five different geographic locations of Yakut snow sheep as well as 20 specimens of other recognized subspecies were included in this study. We identified 19 haplotypes, two of which belonged to the population from Kharaulakh Ridge. Median-joining network and Bayesian tree analyses revealed that Kharaulakh population clustered separately from all the other Yakut snow sheep. The divergence time between Kharaulakh population and Yakut snow sheep was estimated as 0.48 ± 0.19 MYA. Thus, the study of the mtDNA cytb sequences confirmed the results of genome-wide SNP analysis. Taking into account the high degree of divergence of Kharaulakh snow sheep from other groups, identified by both nuclear and mitochondrial DNA markers, we propose to classify the Kharaulakh population as a separate subspecies.
A Holistic, Model-Predictive Process Control for Friction Stir Welding Processes Including a 1D FDM Multi-Layer Temperature Distribution Model
Authors: Meyer Stefan P. , Fuderer Sebastian , Zaeh Michael F.
Published: March 2021

Friction press joining is an innovative joining process for bonding plastics and metals without additives in an overlap configuration. This paper presents for the first time a model-based approach for designing a multi-variable model predictive control (MPC) for friction press joining. For system modeling, a differential equation based on the heat flows was proposed and modeled as a torque-dependent function. With this model, it is possible to consider cross-effects between the axial force and the friction zone temperature. With this theoretical approach, adaptive model-predictive process control was implemented and validated for different material combinations (EN AW-6082-T6; EN AW-2024-T3; PE-HD; PA6-GF30; PPS-CF). It could be shown that the MPC has excellent control accuracy even when model uncertainties are introduced. Based on these findings, a 1D Finite Differential Method multi-layer model was developed to calculate the temperature in the plastic component, which is not measurable in situ (r = 0.93). These investigations demonstrate the high potential of the multi-variable MPC for plastic-metal direct joining.