bluuebunny/crossref_metadata_2025 huggingface dataset:
The Study Level of the Goitered Gazelle (Artiodactyla, Bovidae) in Azerbaijan
Authors: Sarukhanova S.
Published: September 2021

The Goitered gazelle is the only representative of true antelopes’ subfamily in the Caucasus. It was widely distributed in semi-desert plains and mountain foothills of the country. By the mid XX century the number of gazelles in Azerbaijan declined catastrophically and was about to be extinct. By common efforts of state and international conservation organizations it was possible to significantly restore the historical range and the number of gazelle population in the country. The Goitered gazelle is one of the priority species and much attention paid to its conservation and restoration in Azerbaijan. But the ecology of this species is still not studied properly. The article is devoted to a brief overview of the main literary sources concerning various population aspects of gazelle and the level of their study in Azerbaijan.
Music Video Gothic: Fragmentary Form at the Dawn of MTV
Authors: Momcilovic Drago
Published: September 2021

This article argues that the modern music video at the dawn of the MTV era embraces a logic of the Gothic fragment. Mobilizing an archive of gothic archetypes of haunting and monstrosity, the music video of the early 1980s confronts anxieties about its unshaped aesthetic character and discursive placelessness and its strained connections to absent textual wholes, performance cultures, and marginalized histories. Through a close reading of four seminal music videos from this time period – The Buggles' Video Killed the Radio Star (1979), David Bowie's Ashes to Ashes (1980), Blondie's Rapture (1981), and Michael Jackson's Thriller (1983) – I argue that the early music video incarnates a tradition of production, circulation, and decoding that I want to call Music Video Gothic. This tradition expresses latent concerns about the music video's aesthetic borders and intertextual relations with cultural and career narratives.
The Way Back Home: Self-Authorship, Home, and Intergenerational Bonds in Arabic YA Novels of Return
Authors: Motawy Yasmine
Published: September 2021

This article deals with award-winning Arabic YA novels The Bamboo Stalk by Saud Alsanousi, and Cappuccino by Fatima Sharafeddine, appropriating Marcia Baxter Magolda's term ‘self-authorship’ to trace the role of transnational migration in young adult cognitive growth. The novels twin maturity with increased ambiguity around national affiliation, and this article demonstrates how intergenerational bonds are challenged, rearranged, and maintained by the protagonists as they struggle for their personal rights within a patriarchal system.
Jellyfish, Lions, and Ducks: Sideways Spirals of Growth in Lucy Ellmann'sDucks, Newburyport
Authors: Malewski Anne , Lavery Nick
Published: September 2021

Focusing on the felt experience of consciousness, Lucy Ellmann's novel for adults Ducks, Newburyport (2019) undermines clear distinctions between the human protagonist's child and adult selves. Alongside references to non-human animals by this human narrator, entire sections present a mountain lioness's perspective. This essay places the concept of growing sideways from queer studies and children's literature scholarship into dialogue with cognitive literary studies to examine the human protagonist's spiralling between child/adult and human/non-human.
‘The End Lies in the Beginning’: Embracing Childhood and Old Age in Susan Hill's Ghost NovelsThe Small HandandDolly
Authors: Miquel-Baldellou Marta
Published: September 2021

Susan Hill's novels The Small Hand (2010) and Dolly (2012) evoke Victorian tropes of age inversion drawn from children's literature and ghost narratives that undermine the boundaries established between childhood and old age. Given their neo-Victorian features, Hill's two novels engage in dialogue with these Victorian tropes, but, in comparison, Hill's spectral entities literally denote that these life stages are interrelated and should be embraced.
Building Reputations: The Careers of Mary Field, Margaret Thomson and Kay Mander
Authors: Easen Sarah
Published: September 2021

Film historians have generally concentrated their research of British non-fiction film-making on the male directors and producers of the British documentary movement. This has resulted in the marginalisation of those operating in other non-fiction genres, in particular the many women documentarists who worked on educational, instructional, travel, commercial, government and industrial films from the 1930s to the 1970s. This article examines the histories of three women documentary film-makers to assess why women are frequently missing from the established accounts of the genre and argue for their inclusion. It provides an overview of women in British documentary histories, followed by case studies of three women who worked in the sector: Mary Field, Margaret Thomson and Kay Mander. It investigates their collegial networks and considers the impact of gender discrimination on their careers in order to understand why they have received so little recognition in histories of the British documentary film movement.
Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals
Authors: Guan Linbo , Fan Ping , Liu Xinghui , Liu Rui , Liu Yu , Bai Huai
Published: September 2021

Restoration of proximal tubular cell integrity and function after ischemic injury involves cell migration and proliferation. Endogenous fields are present during embryonic development and wound healing. Electric field (EF)-induced effects on cell migration have been observed in many cell types. This study investigated the effect of physiological direct current EF (dc EF) on the motility of renal epithelial cells. Human renal tubular epithelial (HK-2) and human-derived renal epithelial (HEK-293) cells were exposed to dc EF at physiological magnitude. Cell images were recorded and analyzed using an image analyzer. Cell lysates were used to detect protein expression by western blot. Scratch wounds were created in monolayers of HK-2 cells, and wound areas of cells were measured in response to EF exposure. Cells migrated significantly faster in the presence of an EF and toward the cathode. Application of an EF led to activation of the Erk1/2, p38 MAPK, and Akt signaling pathways. Pharmacological inhibition of Erk1/2, p38 MAPK, and Akt impaired EF-induced migratory responses, such as motility rate and directedness. In addition, exposure of the monolayers to EF enhanced EF-induced HK-2 wound healing. Our results suggest that EFs augment the rate of single renal epithelium migration and induce cell cathodal migration through activation of Erk1/2, p38 MAPK, and Akt signaling. Moreover, exposure of the renal epithelium to EF facilitated closure of in vitro small wounds by enhancing cell migration.
On the BET Surface Area of Nanocellulose Determined Using Volumetric, Gravimetric and Chromatographic Adsorption Methods
Authors: Kondor Anett , Santmarti Alba , Mautner Andreas , Williams Daryl , Bismarck Alexander , Lee Koon-Yang
Published: September 2021

Volumetric N2 adsorption at −196°C is generally accepted as “gold standard” for estimating the Brunauer-Emmet-Teller (BET) surface area of nanocellulose. It is unclear however, whether the BET surface area of nanocellulose obtained at such low temperatures and pressures is meaningful at an absolute sense, as nanocellulose is used at ambient temperature and pressure. In this work, a systematic evaluation of the BET surface area of nanocellulose using highly crystalline bacterial cellulose (BC) as model nanocellulose was undertaken to achieve a comprehensive understanding of the limitations of BET method for nanocellulose. BET surface area obtained using volumetric N2 adsorption at −196°C was compared with the BET surface area acquired from gravimetric experiments based on n-octane adsorption using dynamic vapour sorption (DVS) and n-octane adsorption determined by inverse gas chromatography (iGC), both at 25°C. It was found that the BET surface area calculated from volumetric N2 adsorption data was 25% lower than that of n-octane adsorption at 25°C obtained using DVS and iGC adsorption methods. These results supported the hypothesis that the BET surface area of nanocellulose is both a molecular scale (N2vs n-octane, molecular cross section of 0.162 nm2vs 0.646 nm2) and temperature (−196°C vs 25°C) dependent property. This study also demonstrates the importance of selecting appropriate BET pressure range based on established criteria and would suggest that room temperature measurement is more relevant for many nanocellulose applications.
Circadian Clock Regulates Inflammation and the Development of Neurodegeneration
Authors: Wang Xiao-Lan , Li Lianjian
Published: September 2021

The circadian clock regulates numerous key physiological processes and maintains cellular, tissue, and systemic homeostasis. Disruption of circadian clock machinery influences key activities involved in immune response and brain function. Moreover, Immune activation has been closely linked to neurodegeneration. Here, we review the molecular clock machinery and the diurnal variation of immune activity. We summarize the circadian control of immunity in both central and peripheral immune cells, as well as the circadian regulation of brain cells that are implicated in neurodegeneration. We explore the important role of systemic inflammation on neurodegeneration. The circadian clock modulates cellular metabolism, which could be a mechanism underlying circadian control. We also discuss the circadian interventions implicated in inflammation and neurodegeneration. Targeting circadian clocks could be a potential strategy for the prevention and treatment of inflammation and neurodegenerative diseases.
NKT Cells Contribute to the Control of Microbial Infections
Authors: Vogt Stefan , Mattner Jochen
Published: September 2021

Innate (-like) T lymphocytes such as natural killer T (NKT) cells play a pivotal role in the recognition of microbial infections and their subsequent elimination. They frequently localize to potential sites of pathogen entry at which they survey extracellular and intracellular tissue spaces for microbial antigens. Engagement of their T cell receptors (TCRs) induces an explosive release of different cytokines and chemokines, which often pre-exist as constitutively expressed gene transcripts in NKT cells and underlie their poised effector state. Thus, NKT cells regulate immune cell migration and activation and subsequently, bridge innate and adaptive immune responses. In contrast to conventional T cells, which react to peptide antigens, NKT cells recognize lipids presented by the MHC class I like CD1d molecule on antigen presenting cells (APCs). Furthermore, each NKT cell TCR can recognize various antigen specificities, whereas a conventional T lymphocyte TCR reacts mostly only to one single antigen. These lipid antigens are either intermediates of the intracellular APC`s-own metabolism or originate from the cell wall of different bacteria, fungi or protozoan parasites. The best-characterized subset, the type 1 NKT cell subset expresses a semi-invariant TCR. In contrast, the TCR repertoire of type 2 NKT cells is diverse. Furthermore, NKT cells express a panoply of inhibitory and activating NK cell receptors (NKRs) that contribute to their primarily TCR-mediated rapid, innate like immune activation and even allow an adaption of their immune response in an adoptive like manner. Dueto their primary localization at host-environment interfaces, NKT cells are one of the first immune cells that interact with signals from different microbial pathogens. Vice versa, the mutual exchange with local commensal microbiota shapes also the biology of NKT cells, predominantly in the gastrointestinal tract. Following infection, two main signals drive the activation of NKT cells: first, cognate activation upon TCR ligation by microbial or endogenous lipid antigens; and second, bystander activation due to cytokines. Here we will discuss the role of NKT cells in the control of different microbial infections comparing pathogens expressing lipid ligands in their cell walls to infectious agents inducing endogenous lipid antigen presentation by APCs.
The NF-κB Pathway: Modulation by Entamoeba histolytica and Other Protozoan Parasites
Authors: Chadha Attinder , Chadee Kris
Published: September 2021

Protozoan parasites have led to worldwide devastation because of their ability to cause infectious diseases. They have evolved as successful pathogens in part because of their remarkable and sophisticated ways to evade innate host defenses. This holds true for both intracellular and extracellular parasites that deploy multiple strategies to circumvent innate host defenses for their survival. The different strategies protozoan parasites use include hijacking the host cellular signaling pathways and transcription factors. In particular, the nuclear factor-κB (NF-κB) pathway seems to be an attractive target for different pathogens owing to their central role in regulating prompt innate immune responses in host defense. NF-κB is a ubiquitous transcription factor that plays an indispensable role not only in regulating immediate immune responses against invading pathogens but is also a critical regulator of cell proliferation and survival. The major immunomodulatory components include parasite surface and secreted proteins/enzymes and stimulation of host cells intracellular pathways and inflammatory caspases that directly or indirectly interfere with the NF-κB pathway to thwart immune responses that are directed for containment and/or elimination of the pathogen. To showcase how protozoan parasites exploits the NF-κB signaling pathway, this review highlights recent advances fromEntamoeba histolyticaand other protozoan parasites in contact with host cells that induce outside-in and inside-out signaling to modulate NF-κB in disease pathogenesis and survival in the host.
Genre Determining Prediction: Non-Standard TAM Marking in Football Language
Authors: Egetenmeyer Jakob
Published: September 2021

German and French football language display tense-aspect-mood (TAM) forms which differ from the TAM use in other genres. In German football talk, the present indicative may replace the pluperfect subjunctive. In French reports of football matches, the imperfective past may occur instead of a perfective past tense-aspect form. We argue that the two phenomena share a functional core and are licensed in the same way, which is a direct result of the genre they occur in. More precisely, football match reports adhere to a precise script and specific events are temporally determined in terms of objective time. This allows speakers to exploit a secondary function of TAM forms, namely, they shift the temporal perspective. We argue that it is on the grounds of the genre that comprehenders predict the deviating forms and are also able to decode them. In various corpus studies, we explore the functioning of these phenomena in order to gain insights into their distribution, grammaticalization and their functioning in discourse. Relevant factors are Aktionsart properties, rhetorical relations and their interaction with other TAM forms. This allows us to discuss coping mechanisms on the part of the comprehender. We broaden our understanding of the phenomena, which have only been partly covered for French and up to now seem to have been ignored in German.
A New Aortic Arch Dissection Classification: The Fuwai Classification
Authors: Qiu Juntao , Luo Xinjin , Wu Jinlin , Pan Wei , Chang Qian , Qian Xiangyang , Sun Xiaogang , Wei Bo , Zhang Liang , Liu Shen , Jiang Wenxiang , Yu Cuntao
Published: September 2021

Aims: We describe a new aortic arch dissection (AcD) classification, which we have called the Fuwai classification. We then compare the clinical characteristics and long-term prognoses of different classifications.

Methods: All AcD patients who underwent surgical procedures at Fuwai Hospital from 2010 to 2015 were included in the study. AcD procedures are divided into three types: Fuwai type Cp, Ct, and Cd. Type Cp is defined as the innominate artery or combined with the left carotid artery involved. Type Cd is defined as the left subclavian artery or combined with the left carotid artery involved. All other AcD surgeries are defined as type Ct. The Chi-square test was adopted for the pairwise comparison among the three types. Kaplan-Meier was used for the analysis of long-term survival and survival free of reoperation.

Results: In total, 1,063 AcD patients were enrolled from 2010 to 2015: 54 patients were type Cp, 832 were type Ct, and 177 were type Cd. The highest operation proportion of Cp, Ct and Cd were partial arch replacement, total arch replacement, and TEVAR. The surgical mortality in type Ct was higher compared to type Cd (Ct vs. Cd = 9.38 vs. 1.69%, p < 0.01) and type Cp (Ct vs. Cp = 9.38 vs. 1.85%, p = 0.06). There was no difference in surgical mortality of type Cp and Cd (p = 0.93). There were no significant differences in the long-term survival rates (p = 0.38) and free of aorta-related re-operations (p = 0.19).

Conclusion: The Fuwai classification is used to distinguish different AcDs. Different AcDs have different surgical mortality and use different operation methods, but they have similar long-term results.
Early and Long-Term Follow-Up for Chronic Type B and Type Non-A Non-B Aortic Dissection Using the Frozen Elephant Trunk Technique
Authors: Luo Congcong , Qi Ruidong , Zhong Yongliang , Chen Suwei , Liu Hao , Guo Rutao , Ge Yipeng , Sun Lizhong , Zhu Junming
Published: September 2021

Background: This study aimed to evaluate the early and long-term outcomes of a single center using a frozen elephant trunk (FET) procedure for chronic type B or non-A non-B aortic dissection.

Methods: From February 2009 to December 2019, 79 patients diagnosed with chronic type B or non-A non-B aortic dissection who underwent the FET procedure were included in the present study. We analyzed operation mortality and early and long-term outcomes, including complications, survival and interventions.

Results: The operation mortality rate was 5.1% (4/79). Spinal cord injury occurred in 3.8% (3/79), stroke in 2.5% (2/79), and acute renal failure in 5.1% (4/79). The median follow-up time was 53 months. The overall survival rates were 96.2, 92.3, 88.0, 79.8, and 76.2% at 1/2, 1, 3, 5 and 7 years, respectively. Moreover, 79.3% of patients did not require distal aortic reintervention at 7 years. The overall survival in the subacute group was superior to that in the chronic group (P = 0.047).

Conclusion: The FET technique is a safe and feasible approach for treating chronic type B and non-A non-B aortic dissection in patients who have contraindications for primary endovascular aortic repair. The technique combines the advantages of both open surgical repair and endovascular intervention, providing comparable early and long-term follow-up outcomes and freedom from reintervention.
The Cost of Asking: ‘Say that Again?’: A Social Capital Theory View Into How Lecture Recording Supports Widening Participation
Authors: MacKay Jill R. D. , Nordmann Emily , Murray Leigh , Browitt Alison , Anderson Monika , Hutchison Jacqui
Published: September 2021

Lecture recording, as a form of technology enhanced learning, has been purported to support equality in Higher Education. The introduction of lecture recording is often controversial, with some lecturers having concerns as to how recordings may change teaching and learning. A commonly reported motivation for incorporating lecture recordings is supporting the needs of widening participation students, students who are otherwise under-represented in higher education. In this study, we used focus groups to explore the experiences of widening participation students in higher education as they navigated their university programmes. We held four focus groups in three Scottish universities, and discussed and developed findings alongside a stakeholder group. We then applied a social capital lens to the data to explore whether recordings can be used to overcome a lack of social capital in widening participation students. Our participants identified areas where they lacked social ‘credit’, such as a lack of peer parity among colleagues and experiences which could be described as microaggressions. Students discussed reasons why the ‘cost’ of asking questions in class was too high, and how recordings support them by allowing them to save on this perceived cost. However students also recognised the tension of a lack of trust between lecturers and students, which could be exacerbated by recordings. We found good evidence to support a social capital view of ‘trust as credit’ in interactions between students and lecturers, and provide suggestions for how lecture recording can be used to support widening participation students in this area.
Roles of Skeletal Muscle-Derived Exosomes in Organ Metabolic and Immunological Communication
Authors: Aoi Wataru , Tanimura Yuko
Published: September 2021

Skeletal muscles secrete various factors, such as proteins/peptides, nucleotides, and metabolites, which are referred to as myokines. Many of these factors are transported into extracellular bodily fluids in a free or protein-bound form. Furthermore, several secretory factors have been shown to be wrapped up by small vesicles, particularly exosomes, secreted into circulation, and subsequently regulate recipient cells. Thus, exosome contents can be recognized as myokines. In recipient cells, proteins, microRNAs, and metabolites in exosomes can regulate the expression and activity of target proteins associated with nutrient metabolism and immune function. The levels of circulating exosomes and their contents are altered in muscle disorders and metabolic-related states, such as metabolic dysfunction, sarcopenia, and physical fitness. Therefore, such circulating factors could mediate various interactions between skeletal muscle and other organs and may be useful as biomarkers reflecting physiological and pathological states associated with muscular function. Here, this review summarizes secretory regulation of muscle-derived exosomes. Their metabolic and immunological roles and the significance of their circulating levels are also discussed.
Defensive Behavior and Morphometric Variation in Apis mellifera Colonies From Two Different Agro-Ecological Zones of North-Western Argentina
Authors: Bianchi Eliana , Agra Marcelo Nicolás , García Cristina , Gennari Gerardo , Maldonado Luis , Rodríguez Graciela Adriana , Palacio María Alejandra , Scannapieco Alejandra Carla , Lanzavecchia Silvia Beatriz
Published: September 2021

European lineages of Apis mellifera were first introduced into America for beekeeping purposes. A subsequent introduction and accidental release of A. m. scutellata resulted in hybridization events that gave rise to Africanized populations that rapidly spread throughout the continent. In Argentina, Africanized honey bees (AHBs) have been mostly detected in northern regions of the territory, and represent a valuable genetic resource for the selection of stocks with advantageous characteristics for beekeeping. The objective of the present study was to profile honey bee colonies of wild origin with potential beneficial traits for apiculture using morphological, molecular and behavioral traits. Honey bee colonies chosen for evaluation were located in two different agro-ecological regions in north-western Argentina (Tucumán province): The Chaco Depressed Plain (Leales apiary) and the Piedmont (Famaillá apiary). Each apiary was surveyed three times during the 2017–2018 season (mid-season, wintertime, and early spring) for: brood population, phoretic Varroa level and defensive behavior (run, fly, sting, and hang). At the midpoint of the beekeeping season colonies were also characterized by morphometry (45 variables) and mitochondrial haplotypes (COI–COII intergenic region). Apiaries studied showed similar patterns throughout the beekeeping season, for most of the characteristics monitored. However, significant variation in defensive behavior parameters was found between apiaries at the different times of evaluation. Twelve of 45 morphometric variables also showed significant differences between apiaries. The mitochondrial haplotype analysis revealed a high representation of African A4 and A1 haplotypes (91%) in both apiaries. Haplotype variation was associated with morphometric and behavioral traits. Multivariate analyses [principal component analysis (PCA) and principal coordinate analysis (PCoA)] including morphometric and behavior variables explained 65.3% (PCA) and 48.1% (PCoA) of the variability observed between colonies in the first two components. Several morphometric parameters and “fly” behavior were mainly associated with the separation of the colonies. The results from this study point to a possible association between morphometric and behavioral variation and the adaptation of honey bee colonies to differential agro-ecological conditions. We discuss how the detected variation between apiaries can be used for the selection and preservation of honey bee ecotypes in regional breeding programs.
Modularity in Biological Networks
Authors: Alcalá-Corona Sergio Antonio , Sandoval-Motta Santiago , Espinal-Enríquez Jesús , Hernández-Lemus Enrique
Published: September 2021

Network modeling, from the ecological to the molecular scale has become an essential tool for studying the structure, dynamics and complex behavior of living systems. Graph representations of the relationships between biological components open up a wide variety of methods for discovering the mechanistic and functional properties of biological systems. Many biological networks are organized into a modular structure, so methods to discover such modules are essential if we are to understand the biological system as a whole. However, most of the methods used in biology to this end, have a limited applicability, as they are very specific to the system they were developed for. Conversely, from the statistical physics and network science perspective, graph modularity has been theoretically studied and several methods of a very general nature have been developed. It is our perspective that in particular for the modularity detection problem, biology and theoretical physics/network science are less connected than they should. The central goal of this review is to provide the necessary background and present the most applicable and pertinent methods for community detection in a way that motivates their further usage in biological research.
Circulating Levels of MicroRNAs Associated With Hypertension: A Cross-Sectional Study in Male and Female South African Participants
Authors: Matshazi Don M. , Weale Cecil J. , Erasmus Rajiv T. , Kengne Andre P. , Davids Saarah F. G. , Raghubeer Shanel , Davison Glenda M. , Matsha Tandi E.
Published: September 2021

MicroRNAs are non-coding, post-transcriptional regulators of gene expression and their dysregulation has been associated with development of various diseases, including hypertension. Consequently, understanding their role in the pathogenesis and progression of disease is essential. Prior research focusing on microRNAs in disease has provided a basis for understanding disease prognosis and offered possible channels for therapeutic interventions. Herein, we aimed to investigate possible differences in the expression profiles of five microRNAs in the blood of participants grouped on the basis of their hypertension status. This was done to elucidate the possible roles played by these microRNAs in the development of hypertension. Using quantitative reverse transcription polymerase chain reaction, we evaluated the expression levels of miR-126-3p, 30a-5p, 182-5p, 30e-3p, and 1299 in the whole blood of 1456 participants, normotensive (n = 573), screen-detected hypertensive (n = 304) and known hypertensive (n = 579). The expression of miR-126-3p and 182-5p was significantly higher in known hypertensives relative to both screen-detected hypertensives and normotensives, and also in screen-detected hypertensives vs normotensives. A significant association between the expression of miR-126-3p, 182-5p, and 30a-5p and known hypertension was also evident. This study demonstrated dysregulated miR-126-3p, 182-5p, and 30a-5p expression in hypertension, highlighting the possible efficacy of these microRNAs as targets for the diagnosis of hypertension as well as the development of microRNA-based therapies.
Impact of Endometriosis in Women of Arab Ancestry on: Health-Related Quality of Life, Work Productivity, and Diagnostic Delay
Authors: Mousa Mira , Al-Jefout Moamar , Alsafar Habiba , Becker Christian M. , Zondervan Krina T. , Rahmioglu Nilufer
Published: September 2021

Introduction: Endometriosis has a negative effect on health-related quality of life (HRQoL), wellbeing and daily functioning. Endometriosis is an under-researched condition within non-western populations. Cultural representations are needed to understand the relative roles of societal norms, traditional factors, and religious sensitivities on the impact of endometriosis on HRQoL in various populations. In particular, there is a lack of emphasis placed in understanding the association of HRQoL on endometriosis in Arab women.

Method: In this prospective case-control study, 2,610 Arab ancestry women in the United Arab Emirates were recruited to investigate the impact of endometriosis on HRQoL, diagnostic delay, psychological co-morbidities, work productivity, and physical activity. Participants completed the following standardized, validated questionnaires: Short Form-36 version 2 questionnaire, the World Endometriosis Research Foundation EPHect minimum clinical questionnaire version, and Work Productivity and Activity Impairment questionnaire. Translations to the Arabic language, validated using the forward-backward translation method, of the questionnaires were utilized.

Results: HRQoL scores were significantly impaired in women with endometriosis, as demonstrated in the Physical Composite Scores and Mental Composite Scores in the symptomatic control group (p = 0.001; p = 0.003, respectively) and the asymptomatic control group (p < 0.001; p < 0.001, respectively). Susceptibility and severity of multiple pain syndromes and infertility in women with endometriosis was the main indicator of lower HRQoL. Anxiety (p = 0.007) and depression (p = 0.005) were significantly associated with endometriosis, in comparison to symptomatic controls. The average diagnostic delay was 11.61 years, however single women experience 15.81 years of diagnosis delay, with approximately 18% (n = 15) of the single women experiencing more than a 20-year delay in diagnosis. The intensity of physical activity was not associated with endometriosis, when compared to symptomatic (p = 0.405) or asymptomatic controls (p = 0.144).

Conclusion: For the first time, we provide evidence from a combined hospital, clinic, and population-based study that Arab women with endometriosis experience significant impacts on HRQoL, substantial diagnostic delay after the onset of symptoms, significant association to psychological disorders (anxiety and depression), and a negative impact on work productivity. Future research must focus on understanding the personal and culturally centered beliefs of Arab women to ensure a positive HRQoL trajectory by improving diagnosis and management strategies.
NKp30 Receptor Upregulation in Salivary Glands of Sjögren’s Syndrome Characterizes Ectopic Lymphoid Structures and Is Restricted by Rituximab Treatment
Authors: Pontarini Elena , Sciacca Elisabetta , Grigoriadou Sofia , Rivellese Felice , Lucchesi Davide , Fossati-Jimack Liliane , Coleby Rachel , Chowdhury Farzana , Calcaterra Francesca , Tappuni Anwar , Lewis Myles J. , Fabris Martina , Quartuccio Luca , Bella Silvia Della , Bowman Simon , Pitzalis Costantino , Mavilio Domenico , De Vita Salvatore , Bombardieri Michele
Published: September 2021

Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disease resulting from the inflammatory infiltration of exocrine glands, mainly salivary and lacrimal glands, leading to secretory dysfunction and serious complications including debilitating fatigue, systemic autoimmunity, and lymphoma. Like other autoimmune disorders, a strong interferon (IFN) signature is present among subsets of pSS patients, suggesting the involvement of innate immunity in pSS pathogenesis. NCR3/NKp30 is a natural killer (NK) cell-specific activating receptor regulating the cross talk between NK and dendritic cells including type II IFN secretion upon NK-cell activation. A genetic association between single-nucleotide polymorphisms (SNPs) in the NCR3/NKp30 promoter gene and a higher susceptibility for pSS has been previously described, with pSS patients most frequently carrying the major allele variant associated with a higher NKp30 transcript and IFN-γ release as a consequence of the receptor engagement. In the present study, we combined RNA-sequencing and histology from pSS salivary gland biopsies to better characterize NKp30 (NCR3) and its ligand B7/H6 (NCR3LG1) in pSS salivary gland tissues. Levels of NCR3/NKp30 were significantly increased both in salivary glands and in circulating NK cells of pSS patients compared with sicca controls, especially in salivary glands with organized ectopic lymphoid structures. In line with this observation, a strong correlation between NCR3/NKp30 levels and salivary gland infiltrating immune cells (CD3, CD20) was found. Furthermore, NCR3/NKp30 levels also correlated with higher IFN-γ, Perforin, and Granzyme-B expression in pSS SGs with organized ectopic lymphoid structures, suggesting an activation state of NK cells infiltrating SG tissue. Of note, NKp30+ NK cells accumulated at the border of the inflammatory foci, while the NKp30 ligand, B7/H6, is shown to be expressed mainly by ductal epithelial cells in pSS salivary glands. Finally, immunomodulatory treatment, such as the B-cell depleting agent rituximab, known to reduce the infiltration of immune cells in pSS SGs, prevented the upregulation of NCR3/NKp30 within the glands.
Non-random Co-occurrence of Juvenile White Sharks (Carcharodon carcharias) at Seasonal Aggregation Sites in Southern California
Authors: Anderson James M. , Clevenstine Alyssa J. , Stirling Brian S. , Burns Echelle S. , Meese Emily N. , White Connor F. , Logan Ryan K. , O’Sullivan John , Rex Patrick T. , May Jack , Lyons Kady , Winkler Chuck , García-Rodríguez Emiliano , Sosa-Nishizaki Oscar , Lowe Christopher G.
Published: September 2021

Many terrestrial and aquatic taxa are known to form periodic aggregations, whether across life history or solely during specific life stages, that are generally governed by the availability and distribution of resources. Associations between individuals during such aggregation events are considered random and not driven by social attraction or underlying community structure. White sharks (Carcharodon carcharias) have been described as a species that exhibits resource-driven aggregative behaviors across ontogenetic stages and juvenile white sharks are known to form aggregations at specific nursery sites where individuals may remain for extended periods of time in the presence of other individuals. We hypothesized juvenile white sharks form distinct communities during these critical early phases of ontogeny and discuss how a tendency to co-occur across life stages may be seeded by the formation of these communities in early ontogeny. We present results from a series of social network analyses of 86 juvenile white sharks derived from 6 years of passive acoustic telemetry data in southern California, demonstrating the likelihood of association of tagged juvenile white sharks is greater when sharks are of similar size-classes. Individuals in observed networks exhibited behaviors that best approximated fission-fusion dynamics with spatiotemporally unstable group membership. These results provide evidence of possible non-resource driven co-occurrence and community structure in juvenile white sharks during early life stages.
Functionalized Organic–Inorganic Liposome Nanocomposites for the Effective Photo-Thermal Therapy of Breast Cancer
Authors: Liu Haibin , Zhuang Feng , Zhang Chengjian , Ai Wanzhao , Liu Wei , Zhou Xukun
Published: September 2021

Organic–inorganic nanocomposites for photothermal therapy of cancers emerged as a promising strategy against malignant tumors. However, it is still a big challenge to develop a nanocomposite system that can maximize the synergistic photo-thermal therapy effect as well as preserve high stability for simultaneous delivery of the chemotherapeutic drugs and photo-thermal agents. Here, we have exploited an organic liposome containing inorganic core for co-loading the aggregates of bovine serum albumin (BSA), indocyanine green (ICG), and doxorubicin (DOX), abbreviated as BID-liposomal nanocomposites. The three kinds of substances were aggregated in the core of liposomal nanocomposites through hydrophobic and electrostatic interactions. In vitro characterization shows that BID-liposomal nanocomposites were spherical nanoparticles with size of 30–50 nm and good storage stability. Moreover, BID-Liposomal nanocomposites illustrate the strongest cytotoxicity among all the formulations against murine 4T1 tumor cells. In breast cancer-bearing mouse models, BID liposomes lead to significant improvements in tumor inhibition effects with no obvious toxicity. Therefore, the BID-liposomal nanoparticle is believed to be a promising strategy for chemo-photo-thermal therapy against cancers.
High-Salt Diet Accelerated the Decline of Residual Renal Function in Patients With Peritoneal Dialysis
Authors: Gong Nirong , Zhou Chun , Hu Jianxia , Zhong Xiaohong , Yi Zhixiu , Zhang Tingting , Yang Cong , Lin Yanhong , Tian Jianwei , Qin Xianhui , Hu Liping , Jiang Jianping
Published: September 2021

Objective: This study aims to investigate the relationship between dietary salt intake and residual renal function in peritoneal dialysis (PD) patients.

Methods: The daily salt intake of the patients was calculated based on a 3 day dietary record. Sixty-two patients were divided into three groups: 33 patients in the low salt intake group (salt intake <6.0 g/day), 17 in the medium salt intake group (salt intake 6.0 to <8.0 g/day), and 12 in the high salt intake group (salt intake ≥8.0 g/day). Regular follow-up was conducted every 3 months. Urine volume, peritoneal ultrafiltration volume, and other clinical indicators were recorded. Biochemical indexes were detected to evaluate the changes in residual renal function and peritoneal function during follow-up.

Results: A positive correlation between dietary sodium intake and sodium excretion was found. During 12-month follow-up, a decrease of residual renal function showed a significant difference among the three groups (p = 0.041) (15.3 ± 27.5 vs. 12.5 ± 11.5 vs. 32.9 ± 18.4 L/W/1.73 m2 in the low-, medium-, and high salt intake groups, respectively). Consistently, a higher decline of residual renal function (adjusted β, 20.37; 95% CI, 2.83, 37.91) was found in participants with high salt intake (salt intake ≥8 g/day) compared with those in non-high salt intake.

Conclusion: Our study showed that the sodium excretion by peritoneal dialysis was positively correlated with dietary sodium intake in PD patients. The high salt intake diet (salt intake ≥8 g/day) may lead to a faster decline of residual renal function in PD patients.
A 41-Gene Pair Signature for Predicting the Pathological Response of Locally Advanced Rectal Cancer to Neoadjuvant Chemoradiation
Authors: Xue Zhengfa , Yang Shuxin , Luo Yun , Cai Hao , He Ming , Ding Youping , Lei Lei , Peng Wei , Hong Guini , Guo You
Published: September 2021

Background and Purpose: Pathological response status is a standard reference for the early evaluation of the effect of neoadjuvant chemoradiation (nCRT) on locally advanced rectal cancer (LARC) patients. Various patients respond differently to nCRT, but identifying the pathological response of LARC to nCRT remains a challenge. Therefore, we aimed to identify a signature that can predict the response of LARC to nCRT.

Material and Methods: The gene expression profiles of 111 LARC patients receiving fluorouracil-based nCRT were used to obtain gene pairs with within-sample relative expression orderings related to pathological response. These reversal gene pairs were ranked according to the mean decrease Gini index provided by the random forest algorithm to obtain the signature. This signature was verified in two public cohorts of 46 and 42 samples, and a cohort of 33 samples measured at our laboratory. In addition, the signature was used to predict disease-free survival benefits in a series of colorectal cancer datasets.

Results: A 41-gene pair signature (41-GPS) was identified in the training cohort with an accuracy of 84.68% and an area under the receiver operating characteristic curve (AUC) of 0.94. In the two public test cohorts, the accuracy was 93.37 and 73.81%, with AUCs of 0.97 and 0.86, respectively. In our dataset, the AUC was 0.80. The results of the survival analysis show that 41-GPS plays an effective role in identifying patients who will respond to nCRT and have a better prognosis.

Conclusion: The signature consisting of 41 gene pairs can robustly predict the clinical pathological response of LARC patients to nCRT.
Design and Selection of Engineered Lytic Proteins With Staphylococcus aureus Decolonizing Activity
Authors: Gutiérrez Diana , Rodríguez-Rubio Lorena , Ruas-Madiedo Patricia , Fernández Lucía , Campelo Ana Belén , Briers Yves , Nielsen Martin Weiss , Pedersen Karl , Lavigne Rob , García Pilar , Rodríguez Ana
Published: September 2021

Staphylococcus aureus causes various infections in humans and animals, the skin being the principal reservoir of this pathogen. The widespread occurrence of methicillin-resistant S. aureus (MRSA) limits the elimination and treatment of this pathogen. Phage lytic proteins have been proven as efficient antimicrobials against S. aureus. Here, a set of 12 engineered proteins based on endolysins were conceptualized to select the most optimal following a stepwise funnel approach assessing parameters including turbidity reduction, minimum inhibitory concentration (MIC), time-kill curves, and antibiofilm assays, as well as testing their stability in a broad range of storage conditions (pH, temperature, and ionic strength). The engineered phage lysins LysRODIΔAmi and ClyRODI-H5 showed the highest specific lytic activity (5 to 50 times higher than the rest), exhibited a shelf-life up to 6 months and remained stable at temperatures up to 50°C and in a pH range from 3 to 9. LysRODIΔAmi showed the lower MIC values against all staphylococcal strains tested. Both proteins were able to kill 6 log units of the strain S. aureus Sa9 within 5 min and could remove preformed biofilms (76 and 65%, respectively). Moreover, LysRODIΔAmi could prevent biofilm formation at low protein concentrations (0.15–0.6 μM). Due to its enhanced antibiofilm properties, LysRODIΔAmi was selected to effectively remove S. aureus contamination in both intact and disrupted keratinocyte monolayers. Notably, this protein did not demonstrate any toxicity toward human keratinocytes, even at high concentrations (22.1 μM). Finally, a pig skin ex vivo model was used to evaluate treatment of artificially contaminated pig skin using LysRODIΔAmi (16.5 μg/cm2). Following an early reduction of S. aureus, a second dose of protein completely eradicated S. aureus. Overall, our results suggest that LysRODIΔAmi is a suitable candidate as antimicrobial agent to prevent and treat staphylococcal skin infections.
Identification of Four Secreted Aspartic Protease-Like Proteins Associated With Sophorolipids Synthesis in Starmerella bombicola CGMCC 1576
Authors: Liu Jun , Zhao Guoqin , Zhang Xinyu , Song Xin
Published: September 2021

The non-pathogenic yeast Starmerella bombicola CGMCC 1576 is an efficient producer of sophorolipids (SLs). The lactonic SLs are mainly produced with yeast extract, and the acidic SLs are mainly produced with ammonium sulfate. Naturally produced SLs are a mixture of various lactonic and acidic SLs. Usually, the SL mixture is not well separated technically, and the separation cost is relatively high. In order to reduce the cost of separation, four secreted aspartic protease-like proteins were identified through proteomic analysis of fermentation broth of S. bombicola under different nitrogen source conditions. The coding genes of the four proteins, namely, sapl1, sapl2, sapl3, and sapl4, are of high sequence similarity (above 55%) and included in a gene cluster. The expression of the four genes was significantly upregulated on (NH4)2SO4 compared with that on yeast extract. The four genes were deleted together to generate a strain Δsapl. The titer of SLs in Δsapl reached 60.71 g/L after 5 days of fermentation using (NH4)2SO4 as the nitrogen source and increased by 90% compared with the wild-type strain. The concentration of acidic SLs was 55.84 g/L, accounting for 92% of the total SLs. The yield of SLs from glucose (g/g) by Δsapl was 0.78, much higher than that by wild-type strain (0.47). However, no increase of SLs production was observed in Δsapl under yeast extract condition. Compared with that of the wild-type strain, the expression levels of the key genes for SLs synthesis were all upregulated to varying degrees in Δsapl under (NH4)2SO4 conditions, and particularly, the expression level of ugta1 encoding UDP glucosyltransferase was upregulated by 14.3-fold. The results suggest that the sapl gene cluster is negatively involved in the production of SLs in the case of (NH4)2SO4 by restraining the expression of the key genes involved in SLs synthesis. The Δsapl strain is an excellent producer of high-titer and high-yield acidic SLs.
Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
Authors: Kay Taryn M. , Aplin Cody P. , Simonet Rowan , Beenken Julie , Miller Robert C. , Libal Christin , Boersma Arnold J. , Sheets Erin D. , Heikal Ahmed A.
Published: September 2021

In this report, we have developed a simple approach using single-detector fluorescence autocorrelation spectroscopy (FCS) to investigate the Förster resonance energy transfer (FRET) of genetically encoded, freely diffusing crTC2.1 (mTurquoise2.1–linker–mCitrine) at the single molecule level. We hypothesize that the molecular brightness of the freely diffusing donor (mTurquoise2.1) in the presence of the acceptor (mCitrine) is lower than that of the donor alone due to FRET. To test this hypothesis, the fluorescence fluctuation signal and number of molecules of freely diffusing construct were measured using FCS to calculate the molecular brightness of the donor, excited at 405 nm and detected at 475/50 nm, in the presence and absence of the acceptor. Our results indicate that the molecular brightness of cleaved crTC2.1 in a buffer is larger than that of the intact counterpart under 405-nm excitation. The energy transfer efficiency at the single molecule level is larger and more spread in values as compared with the ensemble-averaging time-resolved fluorescence measurements. In contrast, the molecular brightness of the intact crTC2.1, under 488 nm excitation of the acceptor (531/40 nm detection), is the same or slightly larger than that of the cleaved counterpart. These FCS-FRET measurements on freely diffusing donor-acceptor pairs are independent of the precise time constants associated with autocorrelation curves due to the presence of potential photophysical processes. Ultimately, when used in living cells, the proposed approach would only require a low expression level of these genetically encoded constructs, helping to limit potential interference with the cell machinery.