bluuebunny/crossref_metadata_2025 huggingface dataset:
Complications After Major Surgery for Duodenopancreatic Neuroendocrine Tumors in Patients with MEN1: Results from a Nationwide Cohort
Authors: van Beek Dirk-Jan , Nell Sjoerd , Vorselaars Wessel M. C. M. , Bonsing Bert A. , van Eijck Casper H. J. , van Goor Harry , Nieveen van Dijkum Elisabeth J. , Dejong Cornelis H. C. , Valk Gerlof D. ,   the DutchMEN Study Group (DMSG), Bisschop P. H. , Dekkers O. M. , Drent M. L. , Havekes B. , de Herder W. W. , van der Horst-Schrivers A. N. A. , Pieterman C. R. C. , van de Ven A. C. , Borel Rinkes Inne H. M. , Vriens Menno R.
Published: January 2021

Background

Little is known about complications after major duodenopancreatic surgery for duodenopancreatic neuroendocrine tumors (dpNETs) in multiple endocrine neoplasia type 1 (MEN1). Therefore, the incidence and severity of complications after major surgery for MEN1-related dpNETs were assessed.

Methods

Patients were selected from the population-based Dutch MEN1 database if they had undergone a Whipple procedure or total pancreatectomy from 2003 to 2017. Complications were graded according to the Clavien–Dindo classification (grade III or higher complications were considered a severe complication) and definitions from the International Study Group of Pancreatic Surgery. The Cumulative Complication Index (CCI®) was calculated as the sum of all complications weighted for their severity. Univariable logistic regression was performed to assess potential associations between predictor candidates and a severe complication.

Results

Twenty-seven patients (median age 43 years) underwent a major duodenopancreatic resection, including 14 Whipple procedures and 13 total pancreatectomies. Morbidity and mortality were 100% (27/27) and 4% (1/27), respectively. A severe complication occurred in 17/27 (63%) patients. The median CCI® was 47.8 [range 8.7–100]. Grade B/C pancreatic fistulas, delayed gastric emptying, bile leakage, hemorrhage, and chyle leakage occurred in 7/14 (50%), 10/27 (37%), 1/27 (4%), 7/27 (26%), 3/27 (11%) patients, respectively. Patients with a severe complication had longer operative time and higher blood loss. After Whipple, new-onset endocrine and exocrine insufficiency occurred in 1/13 and 9/14 patients, respectively.

Conclusions

Major duodenopancreatic surgery in MEN1 is associated with a very high risk of severe complications and cumulative burden of complications and should therefore be reserved for a select subgroup of patients with MEN1-related dpNETs.
Project almanac
Authors: Budarina A.O. , Zelko A.S. , Petrova T.E.
Published: January 2021

Проектный альманах – это серийное непериодическое издание, которое призвано познакомить читателей с новинками в сфере проектной деятельности. В этом выпуске представлены социально-педагогические проекты, которые были успешно защищены воспитанниками распределенного педагогического класса «Учитель будущего», обучающимися в Центре развития современных компетенций детей Института образования Балтийского федерального университета им.И.Канта (г.Калининград) в 2020 году, а также предлагаются материалы для их оценки.
Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
Authors: Perdana Bayu Afnovandra , Dharma Abdi , Zakaria Indra Junaidi , SYAFRIZAYANTI Syafrizayanti
Published: January 2021

Abstract. Perdana BA, Dharma A, Zakaria IJ, Syafrizayanti. 2021. Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation, and fatty acid content. Biodiversitas 22: 505-511. Microalgae have capability to produce fatty acid for biofuel, drugs, and nutraceutical foods development. This study was carried out to obtain a new strain candidate for fatty acid production. The methods were used in this study include isolation of microalgae species from freshwater ponds of Andalas University, Padang, Indonesia. Molecular identification of microalgae was carried out with specific 18S rRNA primer, F-P73, and R-P47. Microalgae growth was measured by cell density and optical density method using various wavelengths (400, 500, and 680 nm). Total lipid was extracted using Bligh & Dyer method. Fatty acid analyses were conducted using gas chromatography-mass spectroscopy. Microalgae were isolated i.e Chlorella emersonii MAUA001, Mychonastes rotundus MAUA002, Scenedesmus dimorphus MAUA003, and Scenedesmus armatus MAUA004. The result exhibited M. rotundus was the highest lipid content, it was about 28.8% biomass weight. Fatty acid profiles of microalgae were dominated by monounsaturated (MUFA) and saturated fatty acid (SFA). The highest content of fatty acid species found in C. emersonii with octadecenoic acid (C18:1) was 47.74% total lipid. This work showed that C. emersonii has potential as biodiesel due to high saturated fatty acid.
Sustainability analysis of tomato jellyfish (Crambione mastigophora) fisheries resources management in Saleh Bay Waters, Sumbawa Island, Indonesia
Authors: EVRON ASRIAL  , MUHAMMAD MARZUKI  , HAMID  , Isfatul khasanah Ruly
Published: January 2021

Abstract. Asrial E, Marzuki M, Hamid, Khasanah RI. 2021. Sustainability analysis of tomato jellyfish (Crambione mastigophora) fisheries resources management in Saleh Bay Waters, Sumbawa Island, Indonesia. Biodiversitas 22: 512-520. The tomato jellyfish (Crambione mastigophora) or gullung (local term), is endemic in Saleh Bay, Sumbawa Island, Indonesia and has been utilized as an additional livelihood by fishermen for last two decades. Its commonly caught by 2-4 fishermen/boat using scoop net, wooden fishing vessels, and lamps as attractors and collectors of jellyfish. This study aims to determine the sustainability of jellyfish fisheries management. A survey-dependent method with sampling, observation, dialogue, and documentation techniques was applied for data compilation. The rapid appraisal for jellyfish fisheries - six dimensions (Rapjellyfish-6D), based on Rapfish technique, is utilized for analysis of the sustainability status of jellyfish fisheries management. This paper describes the sustainability analysis results for two of the six dimensions of jellyfish fisheries management, namely the technological dimension (13 attributes) as an input factor and the economic dimension as an output factor (14 attributes). At present, around 8-30 baskets/boat/day of jellyfish mouth-arms are being sold to the buyers in Saleh Bay. This catch's profit is divided between the fishermen (3 parts) and the boat owner (2 parts). The analytical results show the revenue per cost ratio (R/C Ratio) as 4.75 which means that every 1.00 Indonesian Rupiah (IDR) the cost of catching jellyfish will generate IDR 4.75. The breakeven point (BEP)Price is IDR 13,158 and BEPProduction is 3.13 and 8.33 baskets/trip for the assumed price of IDR 80,000 and IDR 30,000. The technological (36.13%) sustainability status and economic (49.64%) dimension is Less Sustainable. The leverage analysis results indicate that the group of sensitive attributes in the technological and economic dimensions, respectively, consists of 5 attributes and 3 attributes. All sensitive attributes have an impact on the low value of management sustainability. The sustainability of jellyfish fisheries management in Saleh Bay has been supported by a choice of environmentally friendly fishing methods. Besides, the mouth-arm price is formed from an oligopsony market system that is not profitable for fishermen. It is suggested to the village government to build an integrated and environmentally friendly scyphozoan facility to neutralize the liquid waste generated from scyphozoan processing.
Medicinal plants used by the indigenous Ati tribe in Tobias Fornier, Antique, Philippines
Authors: Cordero Cecilia , ALEJANDRO GRECEBIO JONATHAN D.
Published: January 2021

Abstract. Cordero CS, Alejandro GJD. 2021. Medicinal plants used by the indigenous Ati tribe in Tobias Fornier, Antique, Philippines. Biodiversitas 22: 521-538. This study documented the medicinal plants used by the indigenous Ati tribe in Sitio Pantad, Brgy. Igcalawagan, Tobias Fornier, Antique. Semi-structured interviews were conducted with the tribal chieftain, council of elders, herb doctors, and other members of the tribe who have indigenous knowledge of using medicinal plants in traditional medicine. The Use Value, Informant Consensus Factor, and Fidelity Level were used to determine the plants’ importance. A total of 108 plant species distributed in 97 genera and 44 families were used by the Ati to treat 67 diseases in 12 categories. The family Fabaceae was best represented with 15 species, followed by Lamiaceae with 12 species, and Asteraceae, Euphorbiaceae, and Poaceae with 5 species each. The most frequently used part was the leaf and the most common method of preparation and administration was decoction that was taken orally. One of the most culturally important medicinal plants was Euphorbia hirta L. with the highest use value (0.59) for treating visual problems, dengue, typhoid fever, and headache. The result of this study serves as an ethnobotanical base for drug research and formulation, as well as creating the needed awareness for preserving ethnomedicine as a safe and effective alternative means in the health care delivery system.
Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
Authors: LAISINA JANE KATHRYNE JOLANDA , MAHARIJAYA AWANG , SOBIR SOBIR , PURWITO AGUS
Published: January 2021

Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.
Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system
Authors: CHAIYASAN PATCHARAPORN , MINGKWAN BOONYADA , JANTARAT SITTHISAK , SUWANNAPOOM CHATMONGKON , CIOFFI MARCELO , LIEHR THOMAS , TALUMPHAI SUCHEELA , TANOMTONG ALONGKLOD , Supiwong Weerayuth
Published: January 2021

Abstract. Chaiyasan P, Mingkwan B, Jantarat S, Suwannapoom C, Cioffi MDB, Liehr T, Talumphai S, Tanomtong A, Supiwong W. 2021. Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system. Biodiversitas 22: 546-554. Karyotype of Java combtail fish, Belontia hasselti, from To Daeng peat swamp forest, Narathiwat Province, southern Thailand, was studied for the first time. Mitotic chromosome preparations were prepared directly from kidney cells from ten male and ten female fish. Conventional staining, NOR banding, and molecular cytogenetics with ﬂuorescence in situ hybridization (FISH) using 5S and 18S rDNAs, as well as microsatellites d(CA)15 and d(CAC)10 as probes were applied. The diploid chromosome number (2n) was 48 and a female heterogametic sex chromosome system (ZZ/ZW) is suggested. The fundamental numbers (NF) were 48 and 49 in males and females, respectively. The karyotype of males comprised 48 telocentric chromosomes while the female ones were composed of one metacentric and 47 telocentric chromosomes. A single Ag-NOR-bearing chromosomal pair was identified. The NOR positions were characterized at the interstitial sub-centromeric region of pair 13, which coincided with signals of 18S rDNA and d(CAC)10 probes. The 5S rDNA signals were located at interstitial sites of the largest telocentric pair. Microsatellite d(CA)15 repeats were highly distributed throughout almost all entire chromosomes except for centromeric regions on some chromosome pairs, including sex chromosomes. The present study is a novel report for a ZZ/ZW sex chromosome system of this fish family in Thailand.
Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
Authors: INGGRAINI MAULIN , NURFAJRIAH SITI , PRIYANTO JEPRI AGUNG , Ilsan Noor Andryan
Published: January 2021

Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.
Vegetative anatomy of three potted Chrysanthemum varieties under various paclobutrazol concentrations
Authors: Lailaty Intani Quarta , Nugroho Laurentius Hartanto
Published: January 2021

Abstract. Lailaty IQ, Nugroho LH. 2021. Vegetative anatomy of three potted Chrysanthemum varieties under various paclobutrazol concentrations. Biodiversitas 22: 563-570. Chrysanthemum is one ornamental plant with high potential to be developed commercially. It has many varieties such as colors, types, and shapes of flowers. Paclobutrazol (PBZ) is one of plant growth regulators used to suppress plant growth. The PBZ action mechanism is to inhibit gibberellin synthesis. The application of PBZ with a certain concentration on Chrysanthemum plants has the potential to form potted Chrysanthemum. The aim of this study was to analyze the anatomical variation of potted mums varieties, i.e. Jaguar Red, Snow White and Fiji White, and also the effect of PBZ with various concentrations on the anatomy of the stems and leaves of three Chrysanthemum varieties. The research was conducted by applying PBZ concentrations of 0, 50, 100, 150 ppm to the three Chrysanthemum varieties and grown for 8 weeks to form potted Mums. Then, the anatomy of stems and leaves was studied using the paraffin and freehand section methods. Data analysis was performed using ANOVA and DMRT (Duncan Multiple Range Test) for anatomical characters in stems and leaves of three Chrysanthemum varieties. The results showed that the length of the epidermal cells of Fiji white variety was longer than those of Snow White and Jaguar Red. Meanwhile, Jaguar Red produced greater stem thickness, stomata, and leaf thickness than those of the other two varieties. PBZ could increase the size of the anatomical parameters of Chrysanthemum stems and leaves for all varieties. The PBZ concentration of 150 ppm increased leaf thickness and stem diameter, as well as tracheal diameter. The PBZ concentration of 100 ppm increased the size of the guard cells, palisade and spongy tissue, also peripheral tissues. Meanwhile, the PBZ concentration of 50 ppm had an effect on the increase in the number of stomata and pith diameter. The optimum PBZ concentration to form potted Chrysanthemum was 150 ppm.
Spatial and temporal overlaps of top predators: Dhole, tiger and leopard, and their potential preys in Huai Kha Khaeng Wildlife Sanctuary, Thailand
Authors: Charaspet Khwanrutai , Sukmasuang Ronglarp - , KHOEWSREE ์Noraset , Pla-ard Mananya , Paansri Paanwaris , Keawdee Boonyatiporn , Chanachai Yuwaluk , BHUMPAKPHAN NARIS
Published: January 2021

Abstract. Charaspet K, Sukmasuang R, Khoewsree N, Pla-Ard M, Paansri P, Keawdee B, Chanachai Y, Bhumpakphan N. 2021. Spatial and temporal overlaps of top predators: Dhole, tiger and leopard, and their potential prey in Huai Kha Khaeng Wildlife Sanctuary, Thailand. Biodiversitas 22: 580-592. The study of the spatial-temporal overlap of top predators and their prey is important to understand competition among predators and predator-prey relationships so that the viable populations of predators and other animals can be sustained. This research aimed to study the abundance of three top predators: Dhole (Cuon alpinus), tiger (Panthera tigris) and leopard (Panthera pardus), and their potential wild prey in Huai Kha Khaeng Wildlife Sanctuary, Thailand, and to investigate the spatial-temporal overlap between those three predators, and their potential wild prey. We tested two significant hypotheses: (i) tiger and leopard had the highest spatial-temporal overlap and the spatial overlap of dhole and leopard was higher in comparison to dhole and tiger due to a higher prey overlap; (ii) the three species of large carnivore avoided spatial-temporal overlap even if they were in the same area. The study was conducted using camera traps. In addition, the spatial-temporal overlap of large carnivores and their potential prey was studied to understand the important species of prey of these large carnivores. The results showed that the spatial overlap of tiger and leopard was the highest. Dhole had significant spatial overlap with leopard, which was higher in comparison to dhole and tiger. A significant temporal overlap of dhole and leopard was not found. Also, a significant temporal overlap of tiger and leopard was not found. The results were based on hypotheses that conformed to the niche overlap index of the potential prey and the temporal overlap coefficient. There were ten important species of potential prey of large carnivores: Sambar deer (Rusa unicolor), red muntjac (Muntiacus muntjak), gaur (Bos gaurus), banteng (Bos javanicus), wild boar (Sus scrofa), Malayan porcupine (Hystrix brachyura), large Indian civet (Viverra zibetha), Asiatic black bear (Ursus thibetanus), pig-tailed macaque (Macaca nemestrina) and red jungle fowl (Gallus gallus). The important recommendation is the protection of the sanctuary from wildfires which spread from the outside in, especially in dry evergreen forest. Food resources of herbivores in the area must be managed, as well as the prevention of epidemics from livestock to wild animals.
Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene
Authors: Budiariati Vista , Susmiati Trini , Munawaroh Siti , Putri Rachmawati Cahyaningtyas Arie , Widayanti Rini Widayanti
Published: January 2021

Abstract. Budiariati V, Susmiati T, Waroh S, Putri RCA, Widayanti R. 2021. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas 22: 593-600. Catfish is one of the most demanding fish in Indonesia and served in a variety of traditional culinary. Due to their identical morphology and close relation between species in the order of Siluriformes, it is quite tricky to distinguish the species. This can be a threat to develop catfish production in Indonesia since there is a wide variety of catfish species in this mega biodiversity country. The study aimed to analyze the genetic diversity of Indonesian indigenous catfish especially those known as Baung fish by local people based on COII gene. The study also aimed to determine the phylogenetic relationship between the samples and compare them with the GenBank data. A total of 24 samples used in this study from 8 different rivers from 3 different islands and two samples were collected from coastal areas. The study results showed that there is genetic diversity of the Indonesian indigenous catfish based on COII gene. The sequences among 24 samples showed that from 691 nucleotides of COII gene, there were very subtle nucleotides differences of samples that originated from Bojonegoro, Magelang, and samples collected from Baru Beach, Yogyakarta. Based on COII amino acid sequences, 6 polymorphic amino acid sites were on-site number 64, 115, 123, 129, 144, and 165. The samples encoded LLB1 and LPB1 from Baru Beach, Yogyakarta, showed highest different amino acids in the six sites. Samples from the river of Central Java, Sumatra, and Kalimantan belonged to Bagridae family and consist of two different species Hemibagrus sp. and Mystus sp while samples from East Java belonged to Pangasiidae family. The Samples from coastal belonged to Ariidae family.
Phytochemicals and antimicrobial analysis of selected medicinal plants from Brunei Darussalam
Authors: TAHA HUSSEIN , AWANG-JAMIL ZULHAMIZAN , AMINUDDIN MUHAMMAD FAIRUZ , BASRI AIDA MARYAM , ZAIDI BARIRAH QURATULAIN , AHMAD NORHAYATI
Published: January 2021

Abstract. Awang-Jamil Z, Aminuddin MF, Zaidi BQ, Basri AM, Ahmad N, Taha H. 2021. Phytochemicals and antimicrobial analysis of selected medicinal plants from Brunei Darussalam. Biodiversitas 22: 601-606. Medicinal plants have been widely used but their pharmacological properties are yet to be fully explored. This study was aimed to determine the phytochemicals and their antimicrobial activity of four selected medicinal plants: Melastoma malabathricum, Merremia borneensis, Pandanus amaryllifolius, and Senna alata from Brunei Darussalam. Phytochemical constituents were evaluated by using gas chromatography-mass spectrometry (GC-MS) and antimicrobial screening was carried out using agar well diffusion method. A number of phytochemicals were identified in the plant extracts, of which the following compounds were present in higher amounts (>10 %): coumaran, hentriacontane, hexatriacontane, 3-hydroxy benzyl alcohol, α-linolenic acid, palmitic acid, catechol, squalene, and α-tocopherol-β-D-mannoside. A concentration-dependent antimicrobial activity was observed in some extracts against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Saccharomyces cerevisiae. Results of this study showed medicinal potential of the four selected plants.
Morphotype diversity of Prorocentrum lima in the western part of Indonesian waters
Authors: Riani Widiarti  , Zamani Neviaty Putri , Bengen Dietriech Geoffrey , Madduppa Hawis
Published: January 2021

Abstract. Widiarti R, Zamani NP, Bengen DG, Madduppa H. 2021. Morphotype diversity of Prorocentrum lima in the western part of Indonesian waters. Biodiversitas 22: 609-616. Prorocentrum lima is one of the toxic benthic dinoflagellates, known to produce various toxins, including okadaic acid and dinophysis toxins. The species have a wide range of morphological variability, and possess morphotype diversity, which makes it essential to have detailed morphology observation for identification and other purposes. However, such comprehensive observation has never been reported from Indonesian waters. This study aims to determine the morphological characteristics (including pore size and number of pores) of P. lima morphotypes, in the western part of Indonesian waters (Bintan Island, Belitung Island, Seribu Islands, and Karimunjawa Islands). The results showed three different and unique morphotypes of P. lima, namely morphotype 1, morphotype 2, and morphotype 3. Three clusters were presented by Cluster Analysis, corresponded to the three morphotypes, which were Belitung Island clusters, Seribu Islands and Karimunjawa Islands cluster, and Bintan Island cluster, respectively. These findings support distribution of P. lima and its potential risk of toxicity in Indonesian waters, which prompts the necessity of conducting future research, to avoid the negative impact.
Stock status of coconut crab (Birgus latro) in Daeo, Morotai Island District, North Maluku, Indonesia
Authors: Serosero Rugaya , SULISTIONO SULISTIONO , BUTET NURLISA A. , RIANI ETTY
Published: January 2021

Abstract. Serosero RH, Sulistiono, Butet NA, Riani E. 2021. Stock status of coconut crab (Birgus latro) in Daeo, Morotai Island District, North Maluku, Indonesia. Biodiversitas 22: 626-634. The populations of coconut crabs (Birgus latro) in nature continue to decline due to their simple capture for human consumption. This study aimed to determine the status of coconut crab stock in Daeo, Morotai Island Regency, North Maluku, Indonesia including estimations of growth parameters and population size. This research was conducted from October 2016 to September 2017. Growth parameters were estimated based on 12 monthly length-frequency samples. Data analysis was carried out using the ELEFAN I method on the FiSAT II program for estimating growth parameters, and the Schnabel method for estimating population size. The results showed that the level of exploitation of male and female coconut crabs in Daeo was high (E > 0.5). The asymptotic length L∞ for males was 56.96 mm thoracic length (TL) and that of females 52.50 mm TL. The growth coefficient K for males was 0.14 and that for females 0.16. Length to is -1.026 (male) and -0.913 (female). The results generated using the Schnabel method suggested that the population size of the coconut crab in Daeo was 7,466 to 73,948 individuals considering a confidence interval of 95%. The recapture of tagged coconut crabs was merely 0. 82%.
Vegetation structure of Sumatran Orangutan (Pongo abelii) habitat in North Sumatra, Indonesia
Authors: Zaitunah Anita , SAMSURI  , Ras Satia
Published: January 2021

Abstract. Zaitunah A, Samsuri, Ras S. 2021. Vegetation structure of Sumatran Orangutan (Pongo abelii) habitat in North Sumatra, Indonesia. Biodiversitas 22: 635-641. Gunung Leuser National Park forest in the Bukit Lawang section is the habitat of Sumatran Orangutan (Pongo abelii). There have been reports of the orangutans visiting the village. As some tree species are required for their sustenance to provide nests and food, there is a need to study species diversity availability in their habitat. Thus, the aim of the research was to analyze the composition and structure of the area's vegetation-this was done for the forestand mixed plantation. The sampling for the measurement of tree parameters was conducted using the line strip method. The strips (width 50 m, length 250 m) were constituted by a sub-plot measuring for seedling, pole, sapling, and trees. Within the sampling area, 181 species were found. Species within Dipterocarpaceae showed higher important value index (IVI) compared to other species in all layers. Shorea parviflora and Shorea ovalis were among the species with higher IVI in all the layers. The presence of species of Dipterocarpaceae and other species preferred by orangutans will support their quality of life. Therefore, orangutans prefer staying in the forest to entering the garden. Thus, it is concluded that their entry into the mixed gardens is related to the garden's proximity to the forest. Conservation efforts are needed to minimize the conflict between man and orangutan in the surrounding area.
The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA
Authors: Fadhilah Qonita Gina , Santoso Iman , YASMAN Yasman
Published: January 2021

Abstract. Fadhilah QG, Santoso I, Yasman. 2021. The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA. Biodiversitas 22: 642-649.  Marine actinomycetes from mangrove ecosystems are known to be potential antifungal-producing isolates against phytopathogenic fungi. The aim of this research was to obtain potential marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum sp. KA. Screening of 15 marine actinomycetes isolates using a dual culture method with a plug technique showed that 80% of isolates have antagonistic activity, represented as a percentage of growth inhibition range from 47.96% to 84.94%. Among 12 potential isolates, six isolates (SM4, SM11, SM14, SM15, SM18, and SM20) were evaluated for delayed antagonistic activity with incubation periods of 6, 9, and 12 days using the plug and streak techniques. The results showed that the percentage of growth inhibition of selected isolates inclined to increase along with the incubation period prior to inoculation of Colletotrichum sp. KA. Delayed antagonist assays using the streak technique resulted in higher inhibition results compared to the plug technique. Furthermore, the non-delayed assays of the two selected isolates, SM11 and SM15, also inhibited Colletotrichum sp. KA 57.99% and 59.88%, respectively. The delayed antagonist assay with a shorter incubation period of the two selected isolates also showed an increased percentage of growth inhibition of Colletotrichum sp. KA. According to our research, the delayed antagonistic assay of marine actinomycetes isolates with a 12-day incubation period using a plug technique was representative to evaluate the percentage of growth inhibition.
The role of coastal biodiversity conservation on sustainability and environmental awareness in mangrove ecosystem of southern Malang, Indonesia
Authors: Abidin Zainal , Setiawan Budi , Muhaimin Abdul Wahib , Shinta Agustina
Published: January 2021

Abstract. Abidin Z, Setiawan B, Muhaimin AW, Shinta A. 2021. The role of coastal biodiversity conservation on sustainability and environmental awareness in mangrove ecosystem of southern Malang, Indonesia. Biodiversitas 22: 648-658. Southern coast of Malang, East Java, Indonesia has several mangrove ecosystems used for ecotourism, such as Clungup Mangrove Conservation Tiga Warna (CMC Tiga Warna). This area has consistently implemented ecotourism principles. Previously, it was assumed to be vulnerable from the damage due to differences in environmental awareness of ecotourists and local communities. This study aims to (i) describe the strategy of coastal biodiversity conservation to enhance environmental awareness and its sustainability; (ii) analyze the role of conservation on sustainability and environmental awareness, with sustainability as a mediating variable between conservation and environmental awareness. Data analysis was conducted with descriptive and Warp Partial Least Square (WarpPLS). The results indicate that empirical facts of conservation, sustainability, and environmental awareness in CMC Tiga Warna are relevant to the result of statistical analysis. Conservation is believed to form the environmental awareness of ecotourists and local communities and to maintain the sustainability of ecotourism destinations. In addition, the sustainability of ecotourism destinations serves as an effective mediator between conservation and environmental awareness, along with the modeling of environmental awareness enhancement in conservation-based coastal ecotourism. The proposed model explains that sustainability and environmental awareness variances are 23% and 25%, respectively. In summary, Destination Marketing and Management Organization of CMC Tiga Warna ecotourism is believed to improve environmental awareness of ecotourist and local communities by strengthening coastal biodiversity conservation programs in ecotourism areas and in maintaining sustainability.
Ethnoecological study on the utilization of plants in Ciletuh-Palabuhanratu Geopark, Sukabumi, West Java, Indonesia
Authors: WULANDARI INDRI , ISKANDAR BUDIAWATI SUPANGKAT , PARIKESIT PARIKESIT , HUDOSO TEGUH , ISKANDAR JOHAN , MEGANTARA ERRI NOVIAR , GUNAWAN ELMA FAUZIAH , SHANIDA SYA SYA
Published: January 2021

Abstract. Wulandari I, Iskandar BS, Parikesit, Hudoso T, Iskandar J, Shanida SS, Megantara EN, Gunawan EF. 2021. Ethnoecological study on the utilization of plants in Ciletuh-Palabuhanratu Geopark, Sukabumi, West Java, Indonesia. Biodiversitas 22: 661-674.  Plants can provide benefits for community as food, medicine, fuel, building materials, furniture, and others. Plant utilization should be conducted sustainably in a region with high importance of geological, biological, and cultural diversity, such as in Ciletuh-Palabuhanratu Geopark, Sukabumi, West Java, Indonesia. This study aimed to reveal: (i) plant utilization by local community in Ciletuh-Palabuhanratu Geopark based on land cover; and (ii) community dependence on the plants being utilized. Method used in this study was mixed of qualitative and quantitative methods using direct observation, semi-structured and structured interviews data collection techniques. The result of study showed that the community in Ciletuh-Palabuhanratu Geopark still uses plants for food (80 species), medicinal plants (73 species), livestock fodder (9 species), firewood (27 species), building materials, furniture (38 species), and others (23 species). The highest plant utilization came from gardens (131 species), home gardens (99 species), natural forests (77 species), dry-paddy fields, and wet-paddy fields (7 species). Generally, various agroecosystems and also forest in Ciletuh-Palabuhanratu Geopark have an important role in providing plant products to fulfill the daily needs of rural people.
The characteristics of fermented purple sweet potato (Ipomoea batatas) and black rice (Oryza sativa) using UV-irradiated Monascus purpureus
Authors: NOVITA ISWIYANTI , OEDJIJONO OEDJIJONO , ASNANI ARI
Published: January 2021

Abstract. Novita I, Oedjijono, Asnani A. 2021. The characteristics of fermented purple sweet potato (Ipomoea batatas) and black rice (Oryza sativa) using UV-irradiated Monascus purpureus. Biodiversitas 22: 684-690. This research aimed to produce Monascus fermented product (MFP) with purple sweet potato (Ipomoea batatas (L.) Lam.) and black rice (Oryza sativa L.) using UV-irradiated Monascus purpureus Went and evaluated the characteristic of its antibacterial activity against Salmonella typhi. M. purpureus was irradiated with UV at l254 nm for 0, 2, 3, and 4 min. The solid-state fermentation process was carried out for 7, 14, and 21 days. The pigments were measured at l390 nm for yellow and l500 nm for red. The ethanol extracts of MFP were analyzed for their antibacterial activity against S. typhi using the Kirby–Bauer method. The results showed that the highest yield of MFP was obtained from MFP–black rice (51.88%) that used UV-irradiated M. purpureus for 2 min and fermentation for 21 days. The highest absorbance value of MFP–purple sweet potato was obtained from UV-irradiated M. purpureus for 3 min, whereas the highest absorbance value of MFP–black rice was obtained with UV-irradiated M. purpureus for 2 min. Ethanol extracts of both MFP3–purple sweet potato and MFP2–black rice showed antibacterial activities against S. typhi with minimum inhibitory concentration values of 0.2 and 0.15 g/mL, respectively. Thin-layer chromatography analysis of the ethanol extract from MFP2–black rice revealed the presence of bioactive saponin and flavonoid. These findings suggest that UV-irradiated M. purpureus was able to use both purple sweet potato and black rice substrates to produce MFP with antibacterial activity against S. typhi.
The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae
Authors: Hadi Mochammad Syamsul , ABADI ABDUL LATIEF , HIMAWAN TOTO , MASRURI MASRURI , LESTARI SAFIRA RIZKA , RAHARDJO BAMBANG TRI , AINI LUQMAN QURATA , SETIAWAN YOGO , TARNO HAGUS
Published: January 2021

Abstract. Hadi MS, Abadi AL, Himawan T, MAsruri, Lestari SR, Rahardjo BT, Aini LQ, Setiawan Y, Tarno H. 2021. The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae. Biodiversitas 22: 702-712. Several species in the order Lepidoptera act as plant pests, one of which is Plutella xylostella. Plutella xylostella is one of the most destructive pests of cabbage and other horticultural crops. The use of chemical insecticides as pest control for P. xylostella causes many problems, such as the increased pest resistance to pesticides. The objectives of this study are:  (i) to obtain and characterize symbiont bacteria in the digestive tract of P. xylostella collected from organic and conventional agriculture soils; (ii) to evaluate the potential of bacterial symbionts in the digestive tract of P. xylostella from organic and conventional soils in degrading the active ingredient of chlorpyrifos insecticide; (iii) To determine the biodegradation process of chlorpyrifos insecticide by symbiont bacteria in the digestive tract of P. xylostella; and (iv) to identify the derivative compounds from the biodegradation of chlorpyrifos insecticide. The results showed 30 symbiont bacteria isolated from the digestive tract of P. xylostella collected from organic soil and 36 symbiont bacteria isolated from the digestive tract of P. xylostella from conventional farming soil. There are 15 species of symbiont bacteria in 5 genera from the digestive tract of P. xylostella from organic and conventional farming capable of degrading the chlorpyrifos insecticide. They are identified as Providencia sp., Pseudomonas sp., Serratia sp., Proteus sp., and Aeromonas sp. Chlorpyrifos-derived compounds from the biodegradation of symbiont bacteria are less toxic than chlorpyrifos compounds.
Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
Authors: Sari Dian Novita , Nasrullah Hasan , Ekasari Julie , Suprayudi Muhammad Agus , Alimuddin Alimuddin
Published: January 2021

Abstract. Sari DN, Nasrullah H, Ekasari J, Suprayudi MA, Alimuddin A. 2021. Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy). Biodiversitas 22: 741-750. Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolism and high glucose homeostatic in fish. The information about the GCK mRNA sequence and its expression is limited in Giant gourami, one of the most important herbivorous aquaculture species in Indonesia. The present study aimed to characterize the GCK mRNA and analyze its mRNA expression and plasma glucose levels after high glucose injection in Giant gourami. We also compared its sequence variability among carnivorous and herbivorous fish. The GCK mRNA was identified using polymerase chain reaction (PCR) method from the fish liver. Its mRNA level was analyzed by real-time PCR (qPCR). Giant gourami GCK mRNA sequence was 2104 nucleotide long, encoding 478 amino acids, and shared high similarity with other fish. GCK was mainly expressed in the liver. The mRNA level of GCK was highly up-regulated after 6 hours of high glucose injection, in-line with the plasma glucose in the blood. There are no major differences observed in the GCK amino acid sequences among Giant gourami and other fish. The knowledge gained from this study could be used as a reference for further exploration of metabolic regulation in Giant gourami.
Species diversity and abundance of scorpions in Ahvaz city, Southwest Iran
Authors: JALILI SHAH MANSOURI NAHID , Akbarzadeh Kamran , JAHANIFARD ELHAM , VAZIRIANZADEH BABAK , Rafinejad Javad
Published: January 2021

Abstract. Mansouri NJS, Akbarzadeh K, Jahanifard E, Vazirianzadeh B, Rafinejad J. 2021. Species diversity and abundance of scorpions in Ahvaz city, Southwest Iran. Biodiversitas 22: 763-768. Arthropods are one of the most important and diverse phyla of the animal kingdom. This phylum includes several classes of insects and arachnids, which are of medical and veterinary importance. Scorpions are at the top of the group of venomous arthropods in terms of having venomous bites and causing death. Scorpions live mainly in tropical and subtropical regions of the world and therefore have higher diversity and richness in these regions. This study aimed to investigate the biodiversity and abundance of scorpions in Ahvaz city, Khuzestan province, Iran. It has the highest species diversity and species richness in Iranian scorpions. Scorpions were collected from five regions in Ahvaz using various methods. In total 237 scorpions were collected and identified. They belonged to two families, seven genera and seven species: Mesobuthus eupeus (65%), Hemiscorpius lepturus (23.2%), Androctonus crassicauda (3.8%), Compsobuthus rugosulus (3.4%), Orthochirus zagrosensis (2.5%), Apistobuthus susanae (1.7%), Buthacus macrocentrus (0.4%). Except for H. lepturus (Hemiscorpiidae), other species belonged to the Buthidae family. That all of them were non-burrowing. M. eupeus was the dominant species diversity analysis indicated higher species richness and species diversity for summer (Margalef = 1.07; Shannon = 1.55). Logistic regression analysis showed that the variables of geographical location, humidity, and temperature had a significant relationship with the abundance of scorpion species, while height had no statistically significant.
First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae ) originating from Bangka and Belitung Islands, Indonesia
Authors: KURNIAWAN ARDIANSYAH , Hariati Anik Martinah , Kurniawan Andi , Wiadnya Dewa Gede Raka
Published: January 2021

Abstract. Kurniawan A, Hariati AM, Kurniawan A, Wiadnya DGR. 2021. First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae) originating from Bangka and Belitung Islands, Indonesia. Biodiversitas 22: 794-802. Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia. The results suggested that individuals from both islands are clustered together with a strong bootstrap value (100%) in a monophyletic clade. The specimens deposited at NCBI as the first Indonesian genetic records. They have a sister lineage to individuals from Sabah, Malaysia, although supported by a low bootstrap value. While geographical connection during the Pleistocene period allowed wide distribution of the species, differences in the evolutionary processes of historical biogeography and environmental changes are the predicted cause of the low genetic similarity. These findings can be used as a sequence reference and basic information for conservation of this species.
Mangrove diversity and suitability assessments for ecotourism in Cimalaya Wetan Coast, Karawang District, Indonesia
Authors: Tjiong Giok Pin , Supriatna Jatna , Takarina Noverita Dian , Tambunan Rudy Parluhutan
Published: January 2021

Abstract. Pin TG, Supriatna J, Takarina ND, Tambunan RP. 2021. Mangrove diversity and suitability assessments for ecotourism in Cimalaya Wetan Coast, Karawang District, Indonesia. Biodiversitas 22: 803-810. Mangrove forest has been a popular ecotourism destination recently. One of the methods to assess the mangrove potential for ecotourism is by using Tourism Suitability Index (TSI). This study was aimed to assess the ecotourism potentials of mangrove forest sizing of 56.43 hectares in Cilamaya Wetan Sub-district, Karawang District, West Java Province, Indonesia using the TSI method. To assess the mangrove variables, 10 sampling stations have been selected across the coast. In each station, 10 sample plots sizing of 10 m x 10 m have been established. The TSI was measured based on mangrove variables including species diversity, density, and thickness. We recorded six mangrove plant species belonging to three plant families. Avicennia alba, Bruguiera gymnorrhiza and Rhizophora stylosa, were common species found along the coast. Shannon-Wiener index (H’) showed that the west and east parts of Cimalaya Wetan had high mangrove diversity compared to the central part. The TSI analysis showed that the most suitable areas of mangrove forests for ecotourism were 32.18 hectares (57.11% of the total area), mainly located in the east part of the coast. Moreover, the moderate and least suitable areas were 19.12 hectares (33.94%) and 5.03 hectares (8.93%), respectively, and located in the central part of the coast.
Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity
Authors: HABSYAH TENGKU SITI , Basyuni Mohammad , SIREGAR LUTHFI A.M. , AFANDI DADANG , SYAHPUTRA INDRA
Published: January 2021

Abstract. Habsyah TS, Basyuni M, Siregar LAM, Afandi D, Syahputra I. 2021. Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity. Biodiversitas 22: 830-837. The aim of this study was to determine the pattern of chemical compounds from the polyisoprenoid group in oil palm plants that were contributing to the lipase activity. The study was conducted in two locations. A sampling of leaves and fruit mesocarp from 90 progenies was conducted at PT. Socfin Indonesia Aek Loba Plantation, Asahan District, North Sumatra, Indonesia. Biochemical analysis of polyisoprenoid was carried out at the Forest Biotechnology Laboratory, Faculty of Forestry, University of Sumatera Utara, Medan. The results showed that the total lipids in leaves tissue ranged from 0.40 to 1.032 mg/g, with the lowest total lipid found in SL-4966 progeny and the highest total lipid found in SL-9737 progeny. Polyisoprenoid values ​​ranged from 1.63 to 10.82 mg/g dry weight. The highest polyisoprenoid content was obtained in SL-10682 progeny, and the lowest was in SL-5045 progeny leaf tissue. The polyisoprenoid ​​in fruit mesocarp tissue ranged from 1.79 to 4.14 mg/g. The lowest polyisoprenoid content (1.79 mg/g) of fruit mesocarp was found in SL-4233 progeny, while the highest (4.14 mg/g) was found in SL-5010 progeny.  Dolichol and polyprenol are equally dominant in palm leaves.  Carbon polyprenol chain lengths ranged from C45-C105, and dolichol ranged from C50-C110. The polyprenol carbon chain lengths in the mesocarp tissues were C65-C120 and dolichol was C65-C125. Polyisoprenoid analysis on oil palm leaf and fruit mesocarp tissue showed that lipid patterns of oil palm leaf and mesocarp were categorized as type II lipid patterns. It provides important information for selecting genotypes of low lipase activity of oil palm plants.