bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Soil Physicochemical Characteristics and As, Cu, Pb and Zn Contamination in Non-Active Mines at the Portuguese Sector of the Iberian Pyrite Belt
Authors: Alvarenga Paula , Mourinha Clarisse , Palma Patrícia , Cruz Nuno , Rodrigues Sónia Morais
Published: August 2022

This study aimed to evaluate soil physicochemical characteristics (pH, electrical conductivity, organic matter, total N, and extractable P and K), and potentially toxic elements (As, Cu, Pb, and Zn), in non-active mines located in the Portuguese sector of the Iberian Pyrite Belt (IPB). A total of 70 sampling sites were surveyed at Aljustrel and Lousal, in areas already rehabilitated, and at São Domingos, where rehabilitation was only beginning. The soils at São Domingos were very heterogeneous, with extreme values for some properties (e.g., minimum soil pH 2.0 and maximum As concentration, 4382.8 mg kg−1 dry weight basis (DW)). Aljustrel was the site that presented soils with a higher total As, Cu, Pb, and Zn concentration (median values: 441.5, 545.9, 1396.8, and 316.5 mg kg−1 DW, respectively), above the soil quality guidelines values proposed by the Portuguese Environmental Agency (18, 230, 120, and 340 mg kg−1 DW, respectively). A principal component analysis identified the most relevant soil properties to explain the data variance, which were the soil pH and Pb total concentration, followed by Cu and Zn total concentrations, allowing a separation of Aljustrel from the other mines. Pearson correlation coefficients revealed very strong associations between Pb and As, markedly found at higher concentrations in São Domingos, whereas Aljustrel had an elevated concentration of As and Pb, but also of Cu and Zn. It is evident the risk that persists in the Aljustrel mine area, which was not alleviated by the “dig, dump, and cover” techniques that were implemented to rehabilitate the area.
Anaerobic Co-Digestion of Sugarcane Leaves, Cow Dung and Food Waste: Focus on Methane Yield and Synergistic Effects
Authors: Xu Sunqiang , Bi Guican , Liu Xiangmeng , Yu Qiang , Li Dong , Yuan Haoran , Chen Yong , Xie Jun
Published: August 2022

Anaerobic co-digestion (AcoD) of food waste (FW) and lignocellulose waste is a promising technology for methane production. This work investigated the methane generation from AcoD of FW, sugarcane leaves (SLs), and cow dung (CD) under mesophilic conditions in a batch test. As for AcoD of two feedstocks (SL and FW or CD and FW), introduction of SL and CD (25%, volatile solid (VS) basis) showed slight improvement in methane production from FW. In contrast, positive synergistic effect (synergy index = 1.03–1.14 > 1) was observed in all the AcoD reactors of the three feedstocks (SL, CD, and FW). The optimum mixing ratio of FW:SL:CD (VS basis) was 85:11.25:3.75 with a synergy index of 1.07, achieving a methane yield rate and methane content of 297.16 mL/g VS and 73.26%, respectively. This group cumulative methane production was an improvement of 110.45 and 444.72% higher than mono-digestion of SL and CD. The biodegradability, soluble chemical oxygen demand (SCOD), and VS removal rate were 56.44, 44.55 and 55.38%, respectively. The optimum results indicated that AcoD of FW, SL, and CD have higher potentials for energy recovery and provided forceful scientific evidence for their energy utilization.
Changes in Functionality of Tenebrio molitor Larvae Fermented by Cordyceps militaris Mycelia
Authors: Ha Neul-I , Mun Seul-Ki , Im Seung-Bin , Jang Ho-Yeol , Jeong Hee-Gyeong , Kang Kyung-Yun , Park Kyung-Wuk , Seo Kyoung-Sun , Ban Seung-Eon , Kim Kyung-Je , Yee Sung-Tae
Published: August 2022

The Food and Agriculture Organization (FAO) has been estimating the potential of insects as human food since 2010, and for this reason, Tenebrio molitor larvae, also called mealworms, have been explored as an alternative protein source for various foods. In this study, in order to increase nutrient contents and improve preference as an alternative protein source, we fermented the T. molitor larvae by Cordyceps militaris mycelia. T. molitor larvae were prepared at optimal conditions for fermentation and fermented with C. militaris mycelia, and we analyzed T. molitor larvae change in functionality with proximate composition, β-glucan, cordycepin, adenosine, and free amino acids content. T. molitor larvae fermented by C. militaris mycelia showed higher total protein, total fiber, and β-glucan content than the unfermented larvae. In addition, the highest cordycepin content (13.75 mg/g) was observed in shaded dried T. molitor larvae fermented by C. militaris mycelia. Additionally, the isolated cordycepin from fermented T. molitor larvae showed similar cytotoxicity as standard cordycepin when treated with PC-9 cells. Therefore, we report that the optimized methods of T. molitor larvae fermented by C. militaris mycelia increase total protein, total fiber, β-glucan and produce the amount of cordycepin content, which can be contributed to healthy food and increase T. molitor larvae utilization.
Effect of Gaseous Citral on Table Grapes Contaminated by Rhizopus oryzae ITEM 18876
Authors: Quintieri Laura , Fancello Francesco , Caputo Leonardo , Sorrentino Andrea , Zara Severino , Lippolis Vincenzo , Cervellieri Salvatore , Fanelli Francesca , Corvino Antonia , Pace Bernardo , Cefola Maria
Published: August 2022

Rhizopus oryzae is responsible for rapidly producing a deliquescent appearance in grape berries, generally favoured by cold chain interruptions. To counteract fruit spoilage and to meet consumer acceptance, innovative strategies based on the application of natural compounds are ongoing. Due to their biological activities, including antimicrobial ones, natural flavour compounds extend the shelf life and improve the nutritional value as well as the organoleptic properties of foods. Thus, in this work, the application of the antimicrobial citral, a flavor component of monoterpenes identified in plant and fruit essential oils, was developed and validated against one spoiler of R. oryzae. Citral, as pure compound, was first investigated in vitro against R. oryzae ITEM 18876; then, concentrations equal to the minimal inhibitory concentration (MIC) and 4-fold MIC (4MIC) value were applied on the table grape cv Italia infected with this strain and stored. The MIC value was equal to 0.0125 μL/cm3; both citral concentrations (0.0125 and 0.05 µL/cm3) were effective in counteracting the microbial decay of infected table grapes over the storage period. The HS-SPME/GC-MS method showed citral persistence in the head space of plastic trays with the infected samples; as expected, a higher content of citral isomers was found in the sample treated with 4MIC value. In conclusion, citral revealed its efficacy to counteract the onset of soft rot by R. oryzae ITEM 18876 under storage conditions. Thus, it could be successfully exploited to develop an active packaging or natural preservatives to extend table grape shelf life without affecting its quality and sensory characteristics, whilst also satisfying the consumer demand for natural preservative agents.
Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
Authors: Fraccalvieri Rosa , Bianco Angelica , Difato Laura Maria , Capozzi Loredana , Del Sambro Laura , Simone Domenico , Catanzariti Roberta , Caruso Marta , Galante Domenico , Normanno Giovanni , Palazzo Lucia , Tempesta Maria , Parisi Antonio
Published: August 2022

Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or food poisoning due to its toxigenic and pathogenic nature. In this study, we identified members of B. cereus groups in 65% of the ice cream samples analyzed, which were characterized based on multi locus variable number tandem repeats analysis (MLVA) and whole genome sequencing (WGS). The MLVA revealed that 36 strains showed different allelic profiles. Analyses of WGS data enabled the identification of three members of the B. cereus group: B. cereus sensu stricto, B. mosaicus and B. thuringiensis. Based on the multi locus sequence typing (MLST) scheme, the strains were classified in 27 sequence types (STs), including ST26 that causes food poisoning. Toxin genes’ detection revealed the presence of the genes encoding nonhemolytic enterotoxin (NHE), hemolysin BL (HBL), cytotoxin K (cytK) and cereulide (ces) in 100%, 44%, 42% and 8% of the strains, respectively. The identification of eleven antimicrobial resistance (AMR) genes predicted the resistance to five different antimicrobials, and the resistance to beta-lactam antibiotics was confirmed with a phenotypic antimicrobial test. Taken together, the results showed that the B. cereus strains isolated from ice cream were a potential hazard for consumer safety.
Relationships between Instrumental and Sensory Quality Indices of Shine Muscat Grapes with Different Harvesting Times
Authors: Choi Kyeong-Ok , Hur Youn Young , Park Seo Jun , Lee Dong Hoon , Kim Su Jin , Im Dongjun
Published: August 2022

The effects of instrumental quality indices on the sensory properties of Shine Muscat grapes harvested 16, 18, 20, and 22 weeks after full bloom (WAFB) were investigated. The berries harvested at 20 and 22 WAFB gained higher sweetness scores than those harvested at 16 and 18 WAFB, showing similar trends to that of total soluble solids (TSS) content. The sourness, astringency, and firmness scores were not significantly different among the samples. The flavor score showed a trend similar to that of sweetness perception. The higher flavor score in the berries harvested at 20 and 22 WAFB seemed to be derived from the development of floral aroma compounds, including linalool and its derivatives, with ripening. Consumer acceptance was highly correlated with sweetness and flavor perceptions. It was concluded that the TSS content and development of floral aroma compounds are the key quality parameters for Shine Muscat grapes, satisfying consumer acceptability in the market.
Chickpea Aquafaba-Based Emulsions as a Fat Replacer in Pound Cake: Impact on Cake Properties and Sensory Analysis
Authors: Grossi Bovi Karatay Graziele , Rebellato Ana Paula , Joy Steel Caroline , Dupas Hubinger Miriam
Published: August 2022

This study evaluates the use of chickpea aquafaba (CA)-based emulsions as a potential substitute for palm oil (PO), using pound cake as a case study. The CA was characterized in terms of pH (6.38 ± 0.01), density (1.02 g mL−1 ± 0.01), color, total soluble solids (6.3 ± 0.2 °Bx), total solids (5.7 ± 0.2%), thermal properties through DSC, and apparent viscosity (2.5 cPa·s−1 ± 0.02 at 300 s−1). Emulsions containing 35, 30, and 25% of CA were produced and applied to cake formulation C1, C2, and C3, respectively. The cake batter was evaluated in terms of apparent density (0.87–1.04 g1 cm−3), rheology, and pH (6.6–6.8). The cakes were evaluated for specific volume, baking loss (8.9–9.5%), color, and symmetry index on day 1, and firmness, water activity (aw), and moisture content (%), after 14 days of storage. The cakes produced with the emulsions were found to have slightly higher specific volume (2.3 cm3 g−1) when compared to the control (C4) produced with PO (2.2 cm3 g−1). The moisture and aw decreased and firmness increased during storage. In terms of formulation (i.e., day 1 for C1, C2, C3, and C4), there was no significant difference for moisture. As for aw, the C4 (0.90) was significantly different from the cakes produced with emulsions (0.91–0.92). The results from the sensory evaluation, carried out with 120 panelists, showed no statistically significant difference between C3 and C4 for the attributes of aroma, color, texture, flavor, and overall impression. Based on our results, it appears that the CA-based emulsions have the potential to replace PO in pound-cake recipes, reducing total and saturated fat.
Evaluation of Nutritional Quality and Sensory Parameters of Meat from Mallard and Four Species of Wild Goose
Authors: Söderquist Pär , Olsson Camilla , Birch Karina , Olsson Viktoria
Published: August 2022

Future challenges concerning protein supply for food and feed include the management of all currently available resources. In Sweden, wildfowl are hunted for several reasons, one of which is to protect growing crops. In this study, meat from wild geese and mallard was evaluated with respect to its quality and sensory parameters. The most pronounced sensory differences were between meat from the barnacle goose and the Canada goose and between meat from mallards that were farmed and born wild. This study also provides measurements of values for the nutritional and heavy metal contents of the meat from these wildfowl species in order to elucidate their possible use as modern foods.
Physico-Chemical Attributes of Lemon Fruits as Affected by Growing Substrate and Rootstock
Authors: Martínez-Nicolas Juan José , Núñez-Gómez Dámaris , Lidón Vicente , Martínez-Font Rafael , Melgarejo Pablo , Hernández Francisca , Legua Pilar
Published: August 2022

Due to its high content of bioactive compounds, the lemon is considered one of the most relevant species around the world. Its great economic importance is motivated, in addition to its fresh consumption, by its applications in the medical, pharmaceutical, and food industries, etc. However, the chemical and nutritional composition of lemon is not constant and can be influenced by external factors such as variety, weather conditions, crop management, etc. Determining the compositional variations of the fruit, essential to defining its potential use, was the main objective of this study. The physicochemical characteristics of the ‘Verna’ lemon were studied as a function of two controlled variables, the growing substrate and the rootstock. For this, 90 lemon trees were cultivated in three rootstocks and three different culture media. Lemon trees cultivated with 50% sediment/peat mix substrate presented a higher total production (590 lemons and 90.53 kg) while this production was 80% lower on trees cultivated with 75% marine sediment. Citrus macrophylla and Citrus aurantium/Citrus sinensis rootstocks showed a significantly higher production than the Citrus aurantium. All the fruits presented a predominantly yellow color appropriate for the market (0 < CI < +5). Nutritional and chemical parameters were consistent with data reported for the ‘Verna’ clones. All the obtained lemons were suitable for marketing and consumption both in fresh and processed forms. The results indicated the limited influence that the studied variables have on the quality parameters of lemon fruits, but they also could confirm the potential of marine sediment as a culture substrate.
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties
Authors: Liu Chuang , Wang Fangzhou , Zhang Rentang
Published: August 2022

An acidic polysaccharide fraction (BJP-4) was isolated from blackened jujube, and its advanced structures and anti-inflammatory activity were investigated. X-ray diffraction showed that BJP-4 exhibits both crystalline and amorphous portions. Atomic force microscopy data suggested that it contains a large number of spherical lumps. Circular dichroism and Congo red experiments revealed that it has no triple-helix conformation. In steady shear flow results, the BJP-4 solution was a pseudoplastic non-Newtonian fluid with acid-base stability. BJP-4 (20 mg/mL) showed liquid-like properties (G″ > G′), while it performed weak gel-like behavior at a high concentration (40 mg/mL) (G′ > G″). The anti-inflammatory effects of BJP-4 were further evaluated through in vitro experiments. BJP-4 could down-regulate the over-secretion of inflammatory factors (NO, IL-6, IL-1β, TNF-α, iNOS and COX-2) in RAW264.7 cells due to LPS stimulation. Moreover, it demonstrated that BJP-4 restrained the NF-κB signal pathway by regulating TLR4 expression, reducing IκBα phosphorylation level and NF-κB p65 nuclear translocation. In summary, this present study contributes to the application of blackened jujube polysaccharides in the foods and medicine field.
The Starch Physicochemical Properties between Superior and Inferior Grains of Japonica Rice under Panicle Nitrogen Fertilizer Determine the Difference in Eating Quality
Authors: Jiang Yan , Chen Yue , Zhao Can , Liu Guangming , Shi Yi , Zhao Lingtian , Wang Yuan , Wang Weiling , Xu Ke , Li Guohui , Dai Qigen , Huo Zhongyang
Published: August 2022

Nitrogen fertilizer is essential for rice growth and development, and topdressing nitrogen fertilizer at panicle stage has a huge impact on rice grain quality. However, the effect of panicle nitrogen fertilizer (PNF) on starch physicochemical properties and fine structure remain unclear. In this study, four PNF levels (0, 60, 120, 180 kg N ha−1) were grown with the same basal and tiller fertilizer (150 kg N ha−1). The starch physicochemical properties, fine structure, texture properties and eating quality of two japonica rice were determined. We found that the content of total protein, crude fat and amylose between superior and inferior grains were significantly different. Compared with inferior grains, superior grains had low relative crystallinity, good pasting characteristics and outstanding eating quality. With the increase of nitrogen application rates, the starch volume mean diameter was lower; the average chain length of amylopectin was longer; and the relative crystallinity of starch was higher. The changes above in starch structure resulted in an increase in starch solubility, swelling power and gelatinization enthalpy, and led to a decrease in retrogradation enthalpy, retrogradation percentage and pasting viscosity, consequently contributing to the increase in hardness and stickiness of rice and the deterioration of taste value. These results indicated that topdressing PNF lengthened the amylopectin chain, decreased starch granule size, enhanced crystallization stability and increased gelatinization enthalpy, which were the direct reasons for the deterioration of cooking and eating quality.
Evaluation of Prebiotics through an In Vitro Gastrointestinal Digestion and Fecal Fermentation Experiment: Further Idea on the Implementation of Machine Learning Technique
Authors: Song Hokyung , Jeon Dabin , Unno Tatsuya
Published: August 2022

Prebiotics are non-digestible food ingredients that promote the growth of beneficial gut microorganisms and foster their activities. The performance of prebiotics has often been tested in mouse models in which the gut ecology differs from that of humans. In this study, we instead performed an in vitro gastrointestinal digestion and fecal fermentation experiment to evaluate the efficiency of eight different prebiotics. Feces obtained from 11 different individuals were used to ferment digested prebiotics. The total DNA from each sample was extracted and sequenced through Illumina MiSeq for microbial community analysis. The amount of short-chain fatty acids was assessed through gas chromatography. We found links between community shifts and the increased amount of short-chain fatty acids after prebiotics treatment. The results from differential abundance analysis showed increases in beneficial gut microorganisms, such as Bifidobacterium, Faeclibacterium, and Agathobacter, after prebiotics treatment. We were also able to construct well-performing machine-learning models that could predict the amount of short-chain fatty acids based on the gut microbial community structure. Finally, we provide an idea for further implementation of machine-learning techniques to find customized prebiotics.
On the Numerical Approximation of Mobile-Immobile Advection-Dispersion Model of Fractional Order Arising from Solute Transport in Porous Media
Authors: Kamran  , Khan Shahzad , Alhazmi Sharifah E. , Alotaibi Fahad M. , Ferrara Massimiliano , Ahmadian Ali
Published: August 2022

The fractional mobile/immobile solute transport model has applications in a wide range of phenomena such as ocean acoustic propagation and heat diffusion. The local radial basis functions (RBFs) method have been applied to many physical and engineering problems because of its simplicity in implementation and its superiority in solving different real-world problems easily. In this article, we propose an efficient local RBFs method coupled with Laplace transform (LT) for approximating the solution of fractional mobile/immobile solute transport model in the sense of Caputo derivative. In our method, first, we employ the LT which reduces the problem to an equivalent time-independent problem. The solution of the transformed problem is then approximated via the local RBF method based on multiquadric kernels. Afterward, the desired solution is represented as a contour integral in the left half complex along a smooth curve. The contour integral is then approximated via the midpoint rule. The main advantage of the LT-RBFs method is the avoiding of time discretization technique due which overcomes the time instability issues, second is its local nature which overcomes the ill-conditioning of the differentiation matrices and the sensitivity of the shape parameter, since the local RBFs method only considers the discretization points in each local domain around the collocation point. Due to this, sparse and well-conditioned differentiation matrices are produced, and third is the low computational cost. The convergence and stability of the numerical scheme are discussed. Some test problems are performed in one and two dimensions to validate our numerical scheme. To check the efficiency, accuracy, and efficacy of the scheme the 2D problems are solved in complex domains. The numerical results confirm the stability and efficiency of the method.
A Theoretical and Numerical Study on Fractional Order Biological Models with Caputo Fabrizio Derivative
Authors: Rahman Mati ur , Althobaiti Ali , Riaz Muhammad Bilal , Al-Duais Fuad S.
Published: August 2022

This article studies a biological population model in the context of a fractional Caputo-Fabrizio operator using double Laplace transform combined with the Adomian method. The conditions for the existence and uniqueness of solution of the problem under consideration is established with the use of the Banach principle and some theorems from fixed point theory. Furthermore, the convergence analysis is presented. For the accuracy and validation of the technique, some applications are presented. The numerical simulations present the obtained approximate solutions with a variety of fractional orders. From the numerical simulations, it is observed that when the fractional order is large, then the population density is also large; on the other hand, population density decreases with the decrease in the fractional order. The obtained results reveal that the considered technique is suitable and highly accurate in terms of the cost of computing, and can be used to analyze a wide range of complex non-linear fractional differential equations.
Glaucoma Treatment and Hydrogel: Current Insights and State of the Art
Authors: Fea Antonio Maria , Novarese Cristina , Caselgrandi Paolo , Boscia Giacomo
Published: August 2022

Aqueous gels formulated using hydrophilic polymers (hydrogels) and those based on stimuli-responsive polymers (in situ gelling or gel-forming systems) attract increasing interest in the treatment of several eye diseases. Their chemical structure enables them to incorporate various ophthalmic medications, achieving their optimal therapeutic doses and providing more clinically relevant time courses (weeks or months as opposed to hours and days), which will inevitably reduce dose frequency, thereby improving patient compliance and clinical outcomes. Due to its chronic course, the treatment of glaucoma may benefit from applying gel technologies as drug-delivering systems and as antifibrotic treatment during and after surgery. Therefore, our purpose is to review current applications of ophthalmic gelling systems with particular emphasis on glaucoma.
The Influence of Aminoalcohols on ZnO Films’ Structure
Authors: Nowak Ewelina , Chłopocka Edyta , Szybowicz Mirosław , Stachowiak Alicja , Koczorowski Wojciech , Piechowiak Daria , Miklaszewski Andrzej
Published: August 2022

Preparing structures with the sol-gel method often requires control of the basal plane of crystallites, crystallite structures, or the appearance of the voids. One of the critical factors in the formation of a layer are additives, such as aminoalcohols, which increase the control of the sol formation reaction. Since aminoalcohols differ in boiling points and alkalinity, their selection may play a significant role in the dynamics of structure formation. The main aim of this work is to examine the properties of ZnO layers grown using different aminoalcohols at different concentration rates. The layers were grown on various substrates, which would provide additional information on the behavior of the layers on a specific substrate, and the mixture was annealed at a relatively low temperature (400 °C). The research was conducted using monoethanolamine (MEA) and diethanolamine (DEA). The aminoalcohols were added to the solutions in equal concentrations. The microscopic image of the structure and the size of the crystallites were determined using micrographs. X-ray diffractometry and Raman spectroscopy were used for structural studies, phase analysis and to establish the purity of the obtained films. UV-vis absorption and photoluminescence were used to evaluate structural defects. This paper shows the influence of the stabilizer on the morphology of samples and the influence of the morphology and structure on the optical properties. The above comparison may allow the preparation of ZnO samples for specific applications.
Formative Assessment of Diagnostic Testing in Family Medicine with Comprehensive MCQ Followed by Certainty-Based Mark
Authors: Herbaux Charles , Dupré Aurélie , Rénier Wendy , Gabellier Ludovic , Chazard Emmanuel , Lambert Philippe , Sobanski Vincent , Gosset Didier , Lacroix Dominique , Truffert Patrick
Published: August 2022

Introduction: The choice of diagnostic tests in front of a given clinical case is a major part of medical reasoning. Failure to prescribe the right test can lead to serious diagnostic errors. Furthermore, unnecessary medical tests are a waste of money and could possibly generate injuries to patients, especially in family medicine. Methods: In an effort to improve the training of our students to the choice of laboratory and imaging studies, we implemented a specific multiple-choice questions (MCQ), called comprehensive MCQ (cMCQ), with a fixed and high number of options matching various basic medical tests, followed by a certainty-based mark (CBM). This tool was used in the assessment of diagnostic test choice in various clinical cases of general practice in 456 sixth-year medical students. Results: The scores were significantly correlated with the traditional exams (standard MCQ), with matched themes. The proportion of “cMCQ/CBM score” variance explained by “standard MCQ score” was 21.3%. The cMCQ placed students in a situation closer to practice reality than standard MCQ. In addition to its usefulness as an assessment tool, those tests had a formative value and allowed students to work on their ability to measure their doubt/certainty in order to develop a reflexive approach, required for their future professional practice. Conclusion: cMCQ followed by CBM is a feasible and reliable evaluation method for the assessment of diagnostic testing.
Effectiveness of Expressive Writing in Kidney Transplanted Patients: A Randomized Controlled Trial Study
Authors: Pierro Laura , Servidei Giulia , Pretagostini Renzo , Stabile Davide , Nudo Francesco , Lai Silvia , Aceto Paola , Poli Luca , Fazzari Erika , Lai Carlo
Published: August 2022

The present study aimed to assess the effectiveness of an expressive writing (EW) intervention on psychological and physiological variables after kidney transplant. The final sample of 26 were randomly assigned to an expressive writing group (EWG) and control group (CG). Outcomes were focused on depression, anxiety, alexithymia, empathy, resilience, locus of control, creatinine, CDK-EPI, and azotemia. Depressive symptoms and alexithymia levels decreased in the EWG, with better adherence. Resilience declined over time in both groups. The EWG showed a significantly higher CDK-EPI, indicating better renal functioning. EW seems an effective intervention to improve the psychological health of transplanted patients, with a possible effect on renal functioning. These findings open the possibility of planning brief psychological interventions aimed at processing emotional involvement, in order to increase adherence, the acceptance of the organ, and savings in healthcare costs.
Determination of the Best Planting Season for the Protected Cultivation of Papaya
Authors: Salinas Irene , Hueso Juan José , Cuevas Julián
Published: August 2022

Papaya is a tropical crop increasingly cultivated in the greenhouses of subtropical regions such as South East Spain, where the determination of the best planting season is important to ensure a stable fruit production and quality during the year. In this work, we studied plant growth, yield, and fruit quality, comparing spring and autumn planting seasons in ‘Intenzza’ cultivar. The results showed that planting in spring favors plant growth, leading to an earlier entry into production. Total yield and fruit quality were similar in both planting seasons, although the spring cycle provided higher profits due to greater commercial yield and lower discards. Our results confirm that adverse environmental conditions affect the crop in a similar way regardless of the planting season, so different growing cycles are not very useful for filling the production gaps and fighting against seasonality in our region, unless unfavorable climate conditions are avoided inside the greenhouse.
Characterization of the Residue (Endocarp) of Acrocomia aculeata and Its Biochars as a Potential Source for Soilless Growing Media
Authors: León-Ovelar Regina , Fernández-Boy M. Elena , Knicker Heike
Published: August 2022

Endocarp residues remaining after coconut oil extraction from Acrocomia aculeate are traditionally used for combustion as fuel. As an alternative, we propose its conversion into biochar to substitute peat in planting substrates. To test the feasibility of this approach, untreated endocarp residues (particle size approx. 10 mm) and materials shredded into particles of 4 and 1 mm were pyrolyzed at 200 °C, 325 °C and 450 °C and were subsequently characterized. The pyrolysis-induced loss of organic matter increased the ash contents and pH. Nuclear magnetic resonance (NMR) spectroscopy confirmed the aromatization and a loss of carboxyl C with an increasing pyrolysis temperature. This is commonly associated with an enhanced biochemical recalcitrance. The particle size of the feedstock had no significant effect on the chemical composition or microporosity (BET-adsorption with COs) of the biochars, but affected their water holding capacity. With respect to macro- and micronutrients, only the potassium and Olsen P levels occurred in concentrations that are optimal for tomato seed growth. The low nitrogen level of the products may be advantageous for hydroponic culturing since it allows for a higher flexibility for the adaptation of nutrient contents based on the needs of the used culture.
Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species
Authors: Haida Zainol , Sinniah Uma Rani , Nakasha Jaafar Juju , Hakiman Mansor
Published: August 2022

Curcuma caesia Roxb., commonly known as Kali Haldi or black turmeric, is one of the important species in the genus Curcuma. This species has been classified as one of the endangered Curcuma species due to the drastic decrement of this plant in its natural habitat. C. caesia has been overharvested for various purposes, including bioactive compound extraction to fulfill the pharmaceutical industry demand. Hence, this study was conducted to establish a protocol for the propagation of C. caesia via plant tissue culture techniques. In the shoot induction stage, three basal medium formulations, including Murashige and Skoog (MS medium), the combination of Murashige and Skoog macronutrients and B5 micronutrients (MSB5 medium) and woody plant medium (WPM medium) supplemented with 15 μM of 6-benzylaminopurine (BAP), were used. The results found that the MSB5 medium was the most suitable basal medium formulation for shoot induction of C. caesia. In the subsequent experiment, different types of cytokinin, including BAP, kinetin and 2-iP at concentrations of 5, 10, 15 and 20 μM, were fortified in the MSB5 medium for shoot multiplication. The shoot multiplication was further enhanced by supplementing the MSB5 medium with indole-3-butyric acid (IBA) or 1-napthaleneacetic acid (NAA) at the concentrations of 2, 4, 6 and 8 μM. The results showed that a combination of 15 μM of BAP and 6 μM of IBA significantly increased the shoot multiplication with 100% shoot induction, 3.53 shoots/explant, 10.81 cm of shoot length, 9.57 leaves, 0.486 g of leaves fresh weight and 0.039 g of leaves dry weight. After the multiplication, the rooting stage was carried out by altering the basal medium strength into half and full strength and supplementing with 2.5, 5, 7.5 and 10 μM of indole-3-acetic acid (IAA). The full strength of MSB5 medium supplemented with 5 μM of IAA exhibited the highest number of roots and length of roots, with 6.13 roots and 5.37 cm, respectively. After the rooting stage, the plantlets were successfully acclimatized in the potting medium with the combination of cocopeat and peatmoss, and the ratio of 1:1 was found to produce the highest survival rate with 77.78%. In conclusion, the protocol established in this study could be useful for large-scale raw material production, either for conservation or bioactive compound extraction.
River Flow Measurements Utilizing UAV-Based Surface Velocimetry and Bathymetry Coupled with Sonar
Authors: Koutalakis Paschalis , Zaimes George N.
Published: August 2022

Water velocity and discharge are essential parameters for monitoring water resources sustainably. Datasets acquired from Unoccupied Aerial Systems (UAS) allow for river monitoring at high spatial and temporal resolution, and may be the only alternative in areas that are difficult to access. Image or video-based methods for river flow monitoring have become very popular since they are not time-consuming or expensive in contrast to traditional methods. This study presents a non-contact methodology to estimate streamflow based on data collected from UAS. Both surface velocity and river geometry are measured directly in field conditions via the UAS while streamflow is estimated with a new technique. Specifically, surface velocity is estimated by using image-based velocimetry software while river bathymetry is measured with a floating sonar, tethered like a pendulum to the UAV. Traditional field measurements were collected along the same cross-section of the Aggitis River in Greece in order to assess the accuracy of the remotely sensed velocities, depths, and discharges. Overall, the new technique is very promising for providing accurate UAV-based streamflow results compared to the field data.
Effect of Different Cytokinins on Shoot Outgrowth and Bioactive Compounds Profile of Lemograss Essential Oil
Authors: Camas-Reyes Alberto , Vuelvas-Nolasco Rosalía , Cabrera-Ponce José Luis , Pereyra-Alférez Benito , Molina-Torres Jorge , Martínez-Antonio Agustino
Published: August 2022

Lemongrass (Cymbopogon citratus) essential oil (EO) is a major source of bioactive compounds (BC) with anticancer activity such as α-citral, limonene, geraniol, geranyl acetate, and β-caryophyllene. Comparative studies about cytokinin effects on BC profiles in lemongrass are missing. Here, we evaluated four cytokinins (2iP, tZ, BAP, and KIN) in two different osmotic media, MS-N (3% sucrose, 3 g L−1 Gelrite™) and MS-S (5% sucrose, 5 g L−1 Gelrite™). It results in a higher multiplication rate in BAP containing medium compared to tZ, KIN, and 2iP (p ≤ 0.05). While shoots grown on MS-N/BAP, tZ, and KIN exhibited a highly branching morphology, MS-N/2iP produced a less branching architecture. BC profile analysis of established plants in pots revealed that their maxima production depends on the in vitro shoot growth conditions: i.e., highest content (80%) of α-citral in plants that were cultured in MS-S/BAP (p ≤ 0.05), limonene (41%) in MS-N/2iP, or geranyl acetate (25.79%) in MS-S/2iP. These results indicate that it is possible to increase or address the production of BC in lemongrass by manipulating the cytokinin type and osmotic pressure in culture media. The culture protocol described here is currently successfully applied for somatic embryogenesis induction and genetic transformation in lemongrass.
Pulmonary Vein Isolation in Obese Compared to Non-Obese Patients: Real-Life Experience from a Large Tertiary Center
Authors: Wolfes Julian , Hoppe Daniel , Ellermann Christian , Willy Kevin , Rath Benjamin , Leitz Patrick , Güner Fatih , Köbe Julia , Lange Philipp S. , Eckardt Lars , Frommeyer Gerrit
Published: August 2022

1. Introduction: Pulmonary vein isolation (PVI) is an established procedure used to achieve rhythm control in atrial fibrillation (AF). In obese patients (pts), in whom AF occurs more frequently, a reduced effectiveness of PVI has been observed. Therefore, this study’s aim was to compare the long-term efficacy of PVI between obese and non-obese patients. 2. Methods: We enrolled 111 consecutive pts with a body mass index (BMI) of >30 kg/m2 undergoing PVI from our large registry. Procedural data and outcomes were compared with a matched group of 115 non-obese PVI pts and the long-term outcomes were analyzed. 3. Results: Overall follow-up duration was 314 patient-years in the obese and 378 patient-years in the non-obese group. The follow-up rate was 71% in the obese and 76% in the non-obese group. In both groups, their AF-characteristics did not differ significantly, while known risk factors were significantly more prevalent in the obese group. Procedural characteristics were similar in both groups. During follow-up, the obese pts demonstrated significant weight loss compared to the non-obese pts, while at the same time, the overall recurrence rate during follow-up did not differ significantly between both groups (obese: 39.2% and non-obese: 43.7%). PVI related and long-term complications were comparable between both groups. In the univariate analysis, obesity was not found to be associated with an increased AF recurrence risk. 4. Conclusion: These real-life data demonstrate that obese pts may not show higher AF recurrence rates after PVI compared to pts with normal body weight. Furthermore, PVI was found to be safe and effective in obese patients; thus, a BMI alone may not be a criterion for refusal of PVI.
Cardiac Complications of Hypertensive Emergency: Classification, Diagnosis and Management Challenges
Authors: Talle Mohammed A. , Ngarande Ellen , Doubell Anton F. , Herbst Philip G.
Published: August 2022

While mortality in patients with hypertensive emergency has significantly improved over the past decades, the incidence and complications associated with acute hypertension-mediated organ damage have not followed a similar trend. Hypertensive emergency is characterized by an abrupt surge in blood pressure, mostly occurring in people with pre-existing hypertension to result in acute hypertension-mediated organ damage. Acute hypertension-mediated organ damage commonly affects the cardiovascular system, and present as acute heart failure, myocardial infarction, and less commonly, acute aortic syndrome. Elevated cardiac troponin with or without myocardial infarction is one of the major determinants of outcome in hypertensive emergency. Despite being an established entity distinct from myocardial infarction, myocardial injury has not been systematically studied in hypertensive emergency. The current guidelines on the evaluation and management of hypertensive emergencies limit the cardiac troponin assay to patients presenting with features of myocardial ischemia and acute coronary syndrome, resulting in underdiagnosis, especially of atypical myocardial infarction. In this narrative review, we aimed to give an overview of the epidemiology and pathophysiology of hypertensive emergencies, highlight challenges in the evaluation, classification, and treatment of hypertensive emergency, and propose an algorithm for the evaluation and classification of cardiac acute hypertension-mediated organ damage.
Robot-Assisted Radiofrequency Ablation Combined with Thermodynamic Simulation for Epilepsy Reoperations
Authors: Wang Yu-Chi , Cheng Mei-Yun , Hung Po-Cheng , Kuo Cheng-Yen , Hsieh Hsiang-Yao , Lin Kuang-Lin , Tu Po-Hsun , Wu Chieh-Tsai , Hsu Peng-Wei , Wei Kuo-Chen , Chuang Chi-Cheng
Published: August 2022

Repeat craniotomies to treat recurrent seizures may be difficult, and minimally invasive radiofrequency ablation is an alternative therapy. On the basis of this procedure, we aimed to develop a more reliable methodology which is helpful for institutions where real-time image monitoring or electrophysiologic guidance during ablation are not available. We used simulation combined with a robot-assisted radiofrequency ablation (S-RARFA) protocol to plan and execute brain epileptic tissue lesioning. Trajectories of electrodes were planned on the robot system, and time-dependent thermodynamics was simulated with radiofrequency parameters. Thermal gradient and margin were displayed on a computer to calculate ablation volume with a mathematic equation. Actual volume was measured on images after the ablation. This small series included one pediatric and two adult patients. The remnant hippocampus, corpus callosum, and irritative zone around arteriovenous malformation nidus were all treated with S-RARFA. The mean error percentage of the volume ablated between preoperative simulation and postoperative measurement was 2.4 ± 0.7%. No complications or newly developed neurologic deficits presented postoperatively, and the patients had little postoperative pain and short hospital stays. In this pilot study, we preliminarily verified the feasibility and safety of this novel protocol. As an alternative to traditional surgeries or real-time monitoring, S-RARFA served as successful seizure reoperation with high accuracy, minimal collateral damage, and good seizure control.
Office-Based Structural Autologous Fat Injection Laryngoplasty for Unilateral Vocal Fold Paralysis
Authors: Chen Andy Wei-Ge , Chen Chih-Hua , Lin Tsai-Ming , Chang Angela Chih-Hui , Tsai Tzu-Pei , Chang Shyue-Yih
Published: August 2022

Unilateral vocal fold paralysis (UVFP) is a common cause of incomplete glottic closure, leading to significant somatic and social disabilities. Office-based autologous fat injection laryngoplasty (AFIL) has been proposed as an effective treatment for glottic insufficiency but has not been well-studied for UVFP. We enrolled 23 patients who underwent office-based structural AFIL due to unilateral vocal paralysis at our institution between February 2021 and January 2022. In the procedure, autologous fat was harvested and injected into the vocal fold under the guidance of flexible digital endoscopy for structural fat grafting. The voice handicap index-10 (VHI-10) score and perceptual voice measurements were collected before the operation, 2 weeks postoperatively, and 3 months postoperatively. Twenty-two patients were followed-up for at least 3 months. The VHI-10 score improved significantly from 29.65 ± 8.52 preoperatively to 11.74 ± 7.42 at 2 weeks (p < 0.0001) and 5.36 ± 6.67 at 3 months (p < 0.0001). Significant improvements in grades of dysphonia (p < 0.0001), breathiness (p < 0.0001), and asthenia (p = 0.004) were also noted at 3 months postoperatively when perceptual measurements were investigated. Office-based structural AFIL is an effective treatment for improving voice-related disability for UVFP patients.
Depression and Quality of Life in Patients with Gliomas: A Narrative Review
Authors: Hu Yue , Deng Fang , Zhang Lupeng , Hu Keyue , Liu Shiqi , Zhong Suye , Yang Jun , Zeng Xiaomin , Peng Xiaoning
Published: August 2022

In patients with gliomas, depression is a common complication that may cause severe psychological barriers and deteriorate the patient’s quality of life (QoL). Currently, the Hospital Anxiety and Depression Scale (HADS) is the most commonly used tool to diagnose depression in patients with gliomas. Female sex, unmarried status, low education level, high tumor grade, and a history of mental illness may increase the risks of depression and depressive symptoms in patients with gliomas. The QoL of patients with gliomas can be directly reduced by depression. Therefore, the evaluation and intervention of mood disorders could improve the overall QoL of patients with gliomas. Antidepressant use has become a treatment strategy for patients with gliomas and comorbid depression. This narrative review summarizes the current issues related to depression in patients with gliomas, including the prevalence, risk factors, and diagnostic criteria of depression as well as changes in QoL caused by comorbid depression and antidepressant use. The purpose of this review is to guide clinicians to assess the psychological status of patients with gliomas and to provide clinicians and oncologists with a new treatment strategy to improve the prognosis of such patients.
Predictive Modeling for Suicide-Related Outcomes and Risk Factors among Patients with Pain Conditions: A Systematic Review
Authors: Huang Shu , Lewis Motomori O. , Bao Yuhua , Adekkanattu Prakash , Adkins Lauren E. , Banerjee Samprit , Bian Jiang , Gellad Walid F. , Goodin Amie J. , Luo Yuan , Fairless Jill A. , Walunas Theresa L. , Wilson Debbie L. , Wu Yonghui , Yin Pengfei , Oslin David W. , Pathak Jyotishman , Lo-Ciganic Wei-Hsuan
Published: August 2022

Suicide is a leading cause of death in the US. Patients with pain conditions have higher suicidal risks. In a systematic review searching observational studies from multiple sources (e.g., MEDLINE) from 1 January 2000–12 September 2020, we evaluated existing suicide prediction models’ (SPMs) performance and identified risk factors and their derived data sources among patients with pain conditions. The suicide-related outcomes included suicidal ideation, suicide attempts, suicide deaths, and suicide behaviors. Among the 87 studies included (with 8 SPM studies), 107 suicide risk factors (grouped into 27 categories) were identified. The most frequently occurring risk factor category was depression and their severity (33%). Approximately 20% of the risk factor categories would require identification from data sources beyond structured data (e.g., clinical notes). For 8 SPM studies (only 2 performing validation), the reported prediction metrics/performance varied: C-statistics (n = 3 studies) ranged 0.67–0.84, overall accuracy(n = 5): 0.78–0.96, sensitivity(n = 2): 0.65–0.91, and positive predictive values(n = 3): 0.01–0.43. Using the modified Quality in Prognosis Studies tool to assess the risk of biases, four SPM studies had moderate-to-high risk of biases. This systematic review identified a comprehensive list of risk factors that may improve predicting suicidal risks for patients with pain conditions. Future studies need to examine reasons for performance variations and SPM’s clinical utility.