bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Risk Perception on Anxiety in Emerging Adulthood Under the Local Outbreak of COVID-19: A Conditional Process Analysis
Authors: Fu Haojie , Wang Bin
Published: March 2022

This study aims to explore the influence mechanism of COVID-19 risk perception on anxiety in emerging adulthood in the context of public health events of the second round of COVID-19 outbreaks and provide support for exploring the path of mental health after the normalization of the epidemic situation. An online questionnaire, combined with community social work, was used in this study, and data of 522 emerging adults were collected in February 2021. The Perceived Risk of COVID-19 pandemic scale (PRCPS), the generalized anxiety disorder 7-item (GAD-7) scale, the scale of affect balance, and the connor-davidson resilience scale (CD-RISC) were used to investigate. The results showed that: first, the risk perception of COVID-19 in early adulthood was positively predictive of anxiety symptoms [B = 0.110, p < 0.05, 95%CI = (0.042, 0.176)]. Second, the affective quality of life plays a mediating role between the risk perception of COVID-19 and anxiety [B = 0.108, 95%CI = (0.060, 0.161)]. Thirdly, resilience plays a moderating role between the risk perception of COVID-19 and anxiety, the higher the resilience of emerging adulthood, the weaker effects of the risk perception of COVID-19 negative prediction of anxiety [B = −0.110, p < 0.001, 95%CI = (−0.170, −0.049)]. Therefore, to control the anxiety of emerging adulthood in public health events, we should pay attention to the propaganda and management of epidemic information, improve the quality of life, and attention should be paid to the emerging adulthood with low resilience.
The Association Between Connectedness and Grit Among Thai In-school Adolescents in Urban Chiang Mai, Thailand
Authors: Tangmunkongvorakul Arunrat , Kelly Matthew , Thongpibul Kulvadee , Musumari Patou Masika , Srithanaviboonchai Kriengkrai , Banwell Cathy
Published: March 2022

Aim

To investigate the associations between Grit, connectedness, and parental involvement in Thai adolescents. Grit, perseverance, and passion for long-term goals are predictors of academic success and health. There is a small but developing knowledge of the predictors of Grit in Asia, especially Thailand. This paper investigates the proposition that connectedness and parental involvement are positively associated with Grit.

Method

A total of 2,839 lower secondary (grade 8), higher secondary (grade 11), and vocational (year 12) students from 21 schools in Chiang Mai, Thailand participated in a survey that measured Grit using the Short Grit Scale. Bivariate analysis was conducted using the t-test, ANOVA, or Kruskal–Wallis H test as appropriate. Multiple ordinary least squares linear regression analysis was performed to determine factors associated with Grit.

Results

Satisfactory relationships with teachers (p = 0.01), parental support (p = 0.03), interest in school (p = 0.01), having been asked by parents to do homework (β = −0.69; p = 0.012), and having been told by parents that they had done something bad (β = −1.09; p = 0.02) associated with Grit. These findings can aid in design of tailored interventions to improve Grit in Thai adolescents.
Multiple Logistic Regression Analysis of Smartphone Use in University Students
Authors: Chao Chen-Shen , Li Ming-Hsien , Chang Shih-Pei , Chen Yu-Hsuan
Published: March 2022

Problematic smartphone use (PSU) is an expanded public health heed that requires more study to clarify the influence elements of different populations. The aim of this study was to investigate the relationship between smartphone use, and sleep quality, self-perceived health, and exercise participation in university students. A total of 1,575 Taiwanese undergraduate students from 7 universities participated in the study. Three questionnaires were completed by the study individuals. The results show the overall PSU rate was 11.8%. Average smartphone users were more likely to feel in good health, better sleep quality and less unsatisfactory exercise participation than those who were problematic smartphone users. Multiple logistic regression analysis indicated that PSU, low weekly exercise frequency, and poor sleep quality were significant indicators of poor self-perceived health. We concluded that both low physical activity and PSU did have negative impacts on self-perceived health and sleep quality for undergraduate students.
How Warmth Appeal Affects Persuasion: The Moderating Role of Brand Concepts
Authors: Jin Fei , Zhang Jixuan , Wu Banggang , Zhu Xiaodong
Published: March 2022

In practice, more and more companies are using warmth appeals in their advertisements, but not all warmth appeals can bring the expected results. Grounded in social perception, we propose that consumers’ inferences and behavioral intentions stemming from warmth appeals in advertising are moderated by brand concepts. Specifically, warmth appeal decreases competence inferences and, in turn, behavioral intentions toward the self-enhancement brands. However, it increases warmth inferences and, in turn, behavioral intentions toward self-transcendence brands. We tested our hypotheses through two experimental studies. Experiment 1 demonstrated that for self-enhancement brands, warmth appeals in advertisements decreased brand attitudes and purchase intentions; for self-transcendence brands, warmth appeals in advertisements increased brand attitudes and purchase intentions. Experiment 2 showed further evidence to the proposed effect and tested the mediating effects of warmth perception and competence perception. This research provides significant implications for advertising strategies.
Creative Togetherness. A Joint-Methods Analysis of Collaborative Artistic Performance
Authors: Gesbert Vincent , Hauw Denis , Kempf Adrian , Blauth Alison , Schiavio Andrea
Published: March 2022

In the present study, we combined first-, second-, and third-person levels of analysis to explore the feeling of being and acting together in the context of collaborative artistic performance. Following participation in an international competition held in Czech Republic in 2018, a team of ten artistic swimmers took part in the study. First, a self-assessment instrument was administered to rate the different aspects of togetherness emerging from their collective activity; second, interviews based on video recordings of their performance were conducted individually with all team members; and third, the performance was evaluated by external artistic swimming experts. By combining these levels of analysis in different ways, we explore how changes in togetherness and lived experience in individual behavior may shape, disrupt, and (re-)stabilize joint performance. Our findings suggest that the experience of being and acting together is transient and changing, often alternating phases of decrease and increase in felt togetherness that can be consistently recognized by swimmers and external raters.
Lifetime Cannabis Use Is Not Associated With Negative Beliefs About Medication in Patients With First Treatment Psychosis
Authors: Gjerde Priyanthi B. , Steen Synne W. , Vedal Trude S. J. , Steen Nils Eiel , Reponen Elina J. , Andreassen Ole A. , Steen Vidar M. , Melle Ingrid
Published: March 2022

Objective

Cannabis use is common among patients with psychosis, and along with negative beliefs about medication, it has been found to predict poor adherence to antipsychotic drug treatment. Such lack of adherence to antipsychotic drug treatment increases the risk of poor clinical outcomes and relapse in patients with first treatment for psychosis (FTP). However, to date, it is unclear whether cannabis use may be related to negative perceptions about antipsychotic drug treatment.

Methods

A cross-sectional sample of 265 FTP patients with schizophrenia spectrum disorder underwent extensive clinical assessments. Three measures of cannabis use were obtained: lifetime, current and meeting diagnostic criteria for abuse or addiction. For the primary analyses we focused on lifetime cannabis use. The Beliefs about Medication Questionnaire (BMQ) was employed to assess the patients' specific concerns and perceptions of antipsychotic medications, as well as general beliefs about pharmacotherapy. The relationship between lifetime cannabis use and BMQ scores was investigated with general linear model (GLM) analyses, controlling for age and sex.

Results

Patients with lifetime use of cannabis ≥10 times were more likely to be male, younger at the age of onset of psychosis and with higher levels of alcohol use and daily tobacco smoking, as compared to the non-users (p < 0.05). Neither lifetime use of cannabis, current use nor a cannabis abuse diagnosis was associated with negative beliefs about medicines as measured by the BMQ questionnaire.

Conclusion

Use of cannabis is not linked to negative perceptions about antipsychotic medicines in patients with FTP. Other reasons for poor compliance to antipsychotic drug treatment in cannabis users need to be further investigated.
Impact of Online Courses on University Student Visual Attention During the COVID-19 Pandemic
Authors: Gao Qi , Li Sining
Published: March 2022

Background

Under the threat of COVID-19, many universities offer online courses to avoid student gatherings, which prevent teachers from collecting responses and optimizing courses. This work collected eye movement data to analyze attention allocation and proposed instruction for improving the courses.

Methods

Subjects were recruited to watch three online courses. Meanwhile, their eye movement data were collected through Dikablis Glasses. Mayer’s multimedia cognitive theory was adopted to discriminate the pivotal components of online course, and the Mann–Whitney relevance analysis demonstrated that different representations of courses affected the viewers’ attention differently.

Results

Three subjects watched three different types of political courses. Course 1, which combined text and explanation, attracted the most attention. Course 2 was shown to be less attractive than course 1 and better than course 3, but the subjects were distracted by the animations in course 2. Course 3, which did not use any technique to present learning content, attracts the least attention from the subjects. A correlation analysis shows that course 1 and course 3 have similar results compared with course 2.

Conclusion

Online courses have become a norm during the COVID-19 pandemic. Improving the quality of online courses can effectively reduce the impact of the epidemic on teaching. These experiment results suggest that text + commentary in the design of online courses can effectively attract the attention of the listeners and achieve better learning results. Attention gradually rises in the early stage and then falls after reaching a peak. At this time, the proper introduction of animation can effectively reverse the attention curve, while individual text or commentary results in quickly losing the listener’s attention.
MR-Spectroscopy of GABA and Glutamate/Glutamine Concentrations in Auditory Cortex in Clinical High-Risk for Psychosis Individuals
Authors: Grent-’t-Jong Tineke , Gajwani Ruchika , Gross Joachim , Gumley Andrew I. , Lawrie Stephen M. , Schwannauer Matthias , Schultze-Lutter Frauke , Williams Stephen R. , Uhlhaas Peter J.
Published: March 2022

Psychosis involves changes in GABAergic and glutamatergic neurotransmission in auditory cortex that could be important for understanding sensory deficits and symptoms of psychosis. However, it is currently unclear whether such deficits are present in participants at clinical high-risk for psychosis (CHR-P) and whether they are associated with clinical outcomes. Magnetic Resonance Spectroscopy (MEGAPRESS, 1H-MRS at 3 Tesla) was used to estimate GABA, glutamate, and glutamate-plus-glutamine (Glx) levels in auditory cortex in a large sample of CHR-P (n = 99), CHR-N (clinical high-risk negative, n = 32), and 45 healthy controls. Examined were group differences in metabolite concentrations as well as relationships with clinical symptoms, general cognition, and 1-year follow-up clinical and general functioning in the CHR-P group. Results showed a marginal (p = 0.039) main group effect only for Glx, but not for GABA and glutamate concentrations, and only in left, not right, auditory cortex. This effect did not survive multiple comparison correction, however. Exploratory post-hoc tests revealed that there were significantly lower Glx levels (p = 0.029, uncorrected) in the CHR-P compared to the CHR-N group, but not relative to healthy controls (p = 0.058, uncorrected). Glx levels correlated with the severity of perceptual abnormalities and disorganized speech scores. However, in the CHR-P group, Glx levels did not predict clinical or functional outcomes. Accordingly, the findings from the present study suggest that MRS-measured GABA, glutamate and Glx levels in auditory cortex of CHR-P individuals are largely intact.
Parameterizing the AGN Radius–Luminosity Relation from the Eigenvector 1 Viewpoint
Authors: Panda Swayamtrupta
Published: March 2022

The study of the broad-line region (BLR) using reverberation mapping has allowed us to establish an empirical relation between the size of this line-emitting region and the continuum luminosity that drives the line emission (i.e., the RHβ − L5100 relation). To realize its full potential, the intrinsic scatter in the RHβ − L5100 relation needs to be understood better. The mass accretion rate (or equivalently the Eddington ratio) plays a key role in addressing this problem. On the other hand, the Eigenvector 1 schema has helped to reveal an almost clear connection between the Eddington ratio and the strength of the optical Fe II emission that originates from the BLR. This article aims to reveal the connection between theoretical entities, such as the ionization parameter (U) and cloud mean density (nH) of the BLR, with physical observables obtained directly from the spectra, such as optical Fe II strength (RFeII) that has shown immense potential to trace the accretion rate. We utilize the photoionization code CLOUDY and perform a suite of models to reveal the physical conditions in the low-ionization, dust-free, line-emitting BLR. The key here is the focus on the recovery of the equivalent widths (EWs) for the two low-ionization emission lines—Hβ and the optical Fe II—in addition to the ratio of their EWs, i.e., RFeII. We compare the spectral energy distributions, I Zw 1 and NGC 5548, of prototypical Population A and Population B sources, respectively, in this study. The results from the photoionization modeling are then combined with the existing reverberation-mapped sources with observed RFeII estimates taken from the literature, thus allowing us to assess our analytical formulation to tie together the aforementioned quantities. The recovery of the correct physical conditions in the BLR then suggests that—the BLR “sees” only a very small fraction (∼1–10%) of the original ionizing continuum.
The Improvement of the Modified Starch—Glucomannan—Polyvinyl Alcohol Biothermoplastic Composite Characteristics With Polycaprolactone and Anhydride Maleic Acid
Authors: Harsojuwono Bambang Admadi , Arnata I. Wayan , Hartiati Amna , Setiyo Yohanes , Hatiningsih Sayi , Suriati Luh
Published: March 2022

The purpose of this study was to determine the concentrations of polycaprolactone (PCL) and anhydride maleic acid (AMA) to produce a biothermoplastic composite (BtC) of modified cassava starch–glucomannan–polyvinyl alcohol (MSGPvA) that meets the Indonesian National Standard (SNI) and International Bioplastic Standards such as ISO 527/1B, PCL from the UK, and ASTM 5336 for PLA plastic from Japan. This study measured the tensile strength ratio and Young's modulus of MSGPvA BtC compared to commercial biothermoplastic (CBt), elongation at break, swelling, water vapor transmission rate (WVTR), and biodegradation time. In addition, the surface profile, functional group, crystallinity, and thermal stability were also observed, which were analyzed qualitatively and quantitatively. MSGPvA BtC with 20% PCL and 3.5% AMA was able to increase and improve tensile strength, elongation at break, Young's modulus, swelling, WVTR, and degradation time. MSGPvA BtC with 5% PCL and 0.5% AMA has a transverse surface profile that shows the presence of clear and wavy fibers and an elongated surface profile with indistinct waves, containing the OH functional group at wavenumbers 2,962.66 and 3,448.72 cm−1 and C=O at a wavenumber of 1,735.93 cm−1, and has a low crystallinity degree but relatively high thermal stability. All MSGPvA BtC characteristics with 5% PCL and 0.5% AMA have met the SNI and International Bioplastic Standards (ISO 527/1B, PCL from England, ASTM 5336 for PLA plastic from Japan), except for swelling characteristics. Thus, MSGPvA BtC with 5% PCL and 0.5% AMA has the potential to be used as food packaging material.
Comparison of Clinical Data Between Patients With Complications and Without Complications After Spinal Tuberculosis Surgery: A Propensity Score Matching Analysis
Authors: Chen Liyi , Liu Chong , Ye Zhen , Chen Wuhua , Sun Xuhua , Chen Jiarui , Li Hao , Liang Tuo , Huang Shengsheng , Jiang Jie , Chen Tianyou , Guo Hao , Yao Yuanlin , Liao Shian , Yu Chaojie , Wu Shaofeng , Fan Binguang , Zhan Xinli
Published: March 2022

Purpose

This study used a propensity score matching (PSM) analysis to explore the risk factors of post-operative complications and compared the differences in clinical data between them following spinal tuberculosis surgery.

Methods

The clinical data of patients with spinal tuberculosis were collected in our hospital from June 2012 to June 2021, including general information, laboratory results, surgical information, and hospitalization costs. The data were divided into two groups: complication and without complication groups. The baseline data of the two groups were obtained using the PSM analysis. Univariate and multivariate logistic analyses were used to analyze the differences between the two groups.

Results

A total of 292 patients were included in the PSM analysis: 146 patients with complications and 146 patients without complications. The operation time, incision length, hospital stay, and albumin quantity in the complications group were 162 ± 74.1, 11.2 ± 4.76, 14.7 ± 9.34, and 1.71 ± 2.82, respectively, and those in the without complication group were 138 ± 60.5, 10.2 ± 3.56, 11.7 ± 7.44, and 0.740 ± 2.44, respectively. The laboratory costs, examination costs, guardianship costs, oxygen costs, and total costs in the complications group were higher than those in the without complication group. A significant difference was observed in the albumin quantity by logistic regression analysis (P < 0.05).

Conclusion

Several costs in the complication group were higher than in the without complication group. The albumin quantity may be an independent factor to predict post-operative complications of spinal tuberculosis by logistic regression analysis.
Molecular Epidemiology and Pathogenic Characterization of Novel Chicken Infectious Anemia Viruses in Henan Province of China
Authors: Wang Xin-Wei , Feng Jie , Jin Jia-Xin , Zhu Xiao-Jing , Sun Ai-Jun , Liu Hua-Yuan , Wang Jing-Jing , Wang Rui , Yang Xia , Chen Lu , Liao Yi-Fei , Zhuang Guo-Qing
Published: March 2022

Chicken infectious anemia (CIA) is an immunosuppressive disease caused by the chicken infectious anemia virus (CIAV) resulting in heavy economic losses once an outbreak is established. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, China. A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017–2020; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Our results showed that genome sequence similarity among a total of 12 CIAV isolates was high, and ranged from 97.1 to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8 to 98.6%. However, There were mutations in the locus of the major capsid proteins VP1, VP2, and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a pathogenic strain. Our results reveal that both field infection and non-CIAV vaccines contamination promote the epidemiology of CIAV in China and some dominant epidemic viruses have undergone recombination and evolution. This study provides important information to help with the prevention and control of CIAV in the poultry industry.
Management of Dental Disease in Aardvarks (Orycteropus afer) and Potential Use of Cone-Beam Computed Tomography
Authors: Christman Jane E. , VanderHart Daniel , Colmery Ben , Thompson Joy , Duncan Ann E. , Shellabarger Wynona C.
Published: March 2022

Oral disease involving teeth is a common cause of morbidity in aardvarks (Orycteropus afer) under managed care. Cases can be challenging due to the species’ unique skull and dental anatomy and limited veterinary literature. A retrospective evaluation was performed on dental examinations in nine aardvarks housed at a single zoological institution in the United States between 1995 and 2021. The prevalence of dental disease in this population was 88%, with most cases categorized as mild (4/8). Clinical signs were only seen in three cases. Facial swelling prior to surgery was the most common clinical sign (3/8). Dental pathology was more common in the mandibular teeth (27/38) compared to the maxillary teeth (11/38). Dental abnormalities found upon intraoral examination included the presence of dental points (7/8), crown elongation (3/8), purulent material within the oral cavity (4/8), loose teeth (2/8), periodontal pockets (2/8), and oronasal fistula (1/8). Three patients required dental extractions with a lateral buccostomy approach. Diagnostic imaging was performed in most cases (7/8), with two cases undergoing cone-beam computed tomography (CBCT) to characterize dental pathology that was difficult to fully evaluate with standard radiography. Tomographic findings are described in both cases. CBCT was found to be a helpful tool for diagnosing and characterizing dental disease in aardvarks.
Computer-Vision-Based Indexes for Analyzing Broiler Response to Rearing Environment: A Proof of Concept
Authors: Massari Juliana Maria , de Moura Daniella Jorge , de Alencar Nääs Irenilza , Pereira Danilo Florentino , Branco Tatiane
Published: March 2022

Computer-vision systems for herd detection and monitoring are increasingly present in precision livestock. This technology provides insights into how environmental variations affect the group’s movement pattern. We hypothesize that the cluster and unrest indexes based on computer vision (CV) can simultaneously assess the movement variation of reared broilers under different environmental conditions. The present study is a proof of principle and was carried out with twenty broilers (commercial strain Cobb®), housed in a controlled-environment chamber. The birds were divided into two groups, one housed in an enriched environment and the control. Both groups were subjected to thermal comfort conditions and heat stress. Image analysis of individual or group behavior is the basis for generating animal-monitoring indexes, capable of creating real-time alert systems, predicting welfare, health, environment, and production status. The results obtained in the experiment in a controlled environment allowed the validation of the simultaneous application of cluster and unrest indexes by monitoring the movement of the group of broilers under different environmental conditions. Observational results also suggest that research in more significant proportions should be carried out to evaluate the potential positive impact of environmental enrichment in poultry production. The complexity of the environment is a factor to be considered in creating alert systems for detecting heat stress in broiler production. In large groups, birds’ movement and grouping patterns may differ; therefore, the CV system and indices will need to be recalibrated.
Assisted Reproduction Techniques to Improve Reproduction in a Non-Model Species: The Case of the Arabian Bustard (Ardeotis arabs) Conservation Breeding Program
Authors: Carreira Janaina Torres , Lesobre Loïc , Boullenger Sylvain , Chalah Toni , Lacroix Frédéric , Hingrat Yves
Published: March 2022

Artificial reproductive technologies are highly valuable for ex situ conservation. While Arabian bustard populations are declining and extinct in some parts of the range, the International Fund for Houbara Conservation in the United Arab Emirates implemented a conservation breeding program. Since 2012, a total of 1253 eggs were laid through natural reproduction, 1090 were incubated and 379 of these were fertile (fertility rate of 34.8%), leading to the production of 251 chicks. To improve fertility and acquire crucial knowledge for other endangered large birds, artificial reproduction was implemented in 2018 using fresh, refrigerated, and frozen sperm. A total of 720 ejaculates were collected from 12 birds. We analysed these samples for concentration, volume, motility score (0 to 5), viability (eosin/nigrosine), length, and morphology. The first age at collection was 35.7 ± 18.8 months, mean volume was 89.2 ± 65.3 µL, mean concentration was 928 ± 731 sptz/mL and mean motility score was 2.61 ± 0.95. Morphology analyses revealed a bimodal distribution of sperm length. Five hundred and thirty-five ejaculates were cryopreserved and the initial motility score was 3.4 ± 0.7 and 2.0 ± 0.6 after thawing, while the percentage of normal and intact membrane sperm cells decreased from 88.8 ± 7.5% to 52.9 ± 1%. Sixty-five artificial inseminations were performed, leading to a global fertility rate of 84.3%—more precisely, 85.2% and 83.3%, respectively, for fresh and cryopreserved semen. All methods successfully produced fertile eggs, indicating that artificial insemination is an efficient tool for the conservation and genetic management of the species.
Exposure to Magnetic Fields Changes the Behavioral Pattern in Honeybees (Apis mellifera L.) under Laboratory Conditions
Authors: Migdał Paweł , Berbeć Ewelina , Bieńkowski Paweł , Plotnik Mateusz , Murawska Agnieszka , Latarowski Krzysztof
Published: March 2022

Earth’s magnetic field (MF) plays an important role for many species, including the honeybee, in navigation. Nowadays, much larger alternating fields are emitted by miscellaneous electric infrastructure components, such as transformers and power lines, and the environment is therefore polluted by an anthropogenic electromagnetic field, though little is known regarding its impact on living organisms. The behavior of animals is the first and easiest way to establish the impact of stress. It shows if the animal can detect the exposure and react to it. To investigate this, one-day-old bees were exposed to a 50 Hz magnetic field of induction at 1 mT and 1.7 mT for 10 min, 1 h, and 3 h under laboratory conditions. All groups exposed to the magnetic field showed differences in behavioral patterns. What is more, they presented a behavior absent in the control: loss of balance. There were differences, both in the ratio of behaviors and in the number of bouts—exposed bees more often changed behavior. Occurrence of differences is an indication of the reaction of the honeybee organism to the magnetic field. Loss of balance is a disturbing symptom, and behavior changes indicate a disturbance of the honeybee by the electromagnetic field.
Hepatic Enzyme Profile in Horses
Authors: Satué Katy , Miguel-Pastor Laura , Chicharro Deborah , Gardón Juan Carlos
Published: March 2022

For diagnostic purposes, liver enzymes are usually classified into hepatocellular and cholestatic. These two groups of equine liver-specific enzymes include sorbitol dehydrogenase (SDH), glutamate dehydrogenase (GLDH), γ-glutamyl transferase (GGT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP). SDH and GLDH mostly reflect hepatocellular injury and cholestasis, while GGT expresses high values in biliary necrosis or hyperplasia. Likewise, AST, LDH, and ALP also reflect hepatocellular and biliary disease, but these enzymes are not liver specific. From the clinical point of view of the course of liver or biliary disease, AST and ALP are indicative of chronic disease, whereas SDH, GGT, and GLDH indicate an acute course. The patterns of enzymatic changes at the blood level are associated with different types of liver pathologies (infectious, inflammatory, metabolic, toxic, etc.). Increases in hepatocellular versus biliary enzyme activities are indicative of a particular process. There are different ways to diagnose alterations at the hepatic level. These include the evaluation of abnormalities in the predominant pattern of hepatocellular versus cholestatic enzyme abnormalities, the mild, moderate, or marked (5–10-fold or >10-fold) increase in enzyme abnormality concerning the upper limit of the reference range, the evolution over time (increase or decrease) and the course of the abnormality (acute or chronic).
A Case of XX Disorder of Sexual Development in a Female-Phenotype Roe Deer (Capreolus capreolus L.) Associated with Antlers Growth with Retained Velvet
Authors: Silva Filipe , Pires Isabel , Prada Justina , Queirós Miguel , Monzón Aurora , Almeida José , Sargo Roberto , Loureiro Filipa , Sousa Luís , Valente Joana , Viegas Carlos , Ginja Mário , Bastos Estela , Martins-Bessa Ana , Dias Isabel
Published: March 2022

A 3-to-4-year-old roe deer (Capreolus capreolus L.) was admitted to the Veterinary Hospital. Although it showed well-developed antlers with retained velvet, an external female appearance and genitalia were evident. External biometrical measurements were taken for the antlers, and a computed tomography was performed. Molecular studies targeting the SRY gene were performed, and a PIS (polled intersex syndrome) mutation diagnosis was implemented. The gonads consisted of a right testicle paired with a left ovotestis. Histologically, the ovary-like structures in the ovotestis were functional, but the testis, as the testis-like structure in the ovotestis, did not show active spermatogenesis. No evidence of SRY gene was detected by PCR, suggesting an XX-chromosome constitution. Additionally, polled intersex syndrome (PIS) deletion was not detected in the case under study. The clinical and histopathological findings confirmed the DSD with the presence of a testicle and a contralateral ovotestis.
Quantifying Agricultural Drought Severity for Spring Wheat Based on Response of Leaf Photosynthetic Features to Progressive Soil Drying
Authors: Zhang Kai , Zhang Bo , Zhao Funian
Published: March 2022

Agricultural drought definition focuses on the effect of water deficit during the crop growth period on the final crop yield. However, it is difficult to quantify the dynamic process for agricultural drought precisely during the crop growing season and then relate its impact to the final crop yield. This study was conducted to quantify agricultural drought severity for spring wheat (Triticum aestivum L.) at the jointing stage based on the response of leaf physiological parameters to progressive soil drying. The leaf potential and gas exchange parameters were observed daily using a DewPoint Potential Meter (WP4) and portable photosynthetic apparatus (LI-6400) at the jointing stage of spring wheat for two different water treatments: well water supply and natural drought, respectively. The results showed that the leaf photosynthetic features’ response to available soil water could be classified into five main stages, as the available soil water thresholds were at 0.41, 0.2, 0.12, and 0.04, respectively. We defined those five stages as no agricultural drought, mild agricultural drought, moderate agricultural drought, severe agricultural drought, and extremely severe agricultural drought based on the different mechanisms of the net photosynthesis rate’s response to progressive soil drying. The parameters of three stomatal conductance models, i.e., Ball–Berry, Leuning, and Medlyn, had two apparently different groups of values divided by moderate agricultural drought. This study combined atmosphere–soil–crop as a unit to quantify agricultural drought severity during the crop growth period could be used to model crop growth and development under water deficit conditions and calculate agricultural drought indices in drought research and management.
Risks for Public Health and Social Infrastructure in Russian Arctic under Climate Change and Permafrost Degradation
Authors: Revich Boris A. , Eliseev Dmitry O. , Shaposhnikov Dmitry A.
Published: March 2022

This study analyzes the risks to public health and life quality in the conditions of permafrost degradation caused by the ongoing climate change in the Russian Arctic. There are more than 200 Siberian anthrax cattle burial grounds in the Russian permafrost regions. Permafrost degradation poses the risks of thawing of frozen carcasses of the infected animals and propagation of infectious diseases. Permafrost degradation leads to infiltration of toxic waste in the environment. Such waste contains mercury, which migrates into the rivers and forms methylmercury (MeHg) in fish. Other risks associated with permafrost degradation include damage to the existing social infrastructure (housing, health-care facilities, roads, etc.). Various risks to public well-being that emerge because of permafrost degradation were addressed in this study. Relative hazard indices were developed and calculated to characterize the probability of outbreaks of Siberian anthrax in the future. These indices linked the rates of permafrost degradation and the number of Siberian anthrax cattle burials to the potential hazard of re-emergence of Siberian anthrax among local populations in 70 municipal districts under the ongoing warming. The expected damage to public housing, health-care facilities, and motorways was assessed. Accessibility of health care in various regions of the Russian Arctic was analyzed. The economic costs associated with various scenarios of possible destruction of residential buildings, health-care facilities, and roads built on permafrost were estimated.
Detection of Drought Events in Setúbal District: Comparison between Drought Indices
Authors: Silva Tiago , Pires Vanda , Cota Tânia , Silva Álvaro
Published: March 2022

Due to the lack of a general drought definition, water users and managers have developed and used different indices. Many studies using drought indices have been made so as to detect drought events or just to compare their results and assess their advantages and disadvantages. In Portugal, these studies have been done for common drought indices; however, an integrated evaluation and comparison using recent data is needed. Therefore, this study is intended to give an updated overview of the behaviour of the proposed indices. This study proposes the usage of PDSI, scPDSI, SPI and SPEI. With the exception of the PDSI, all indices have been calculated through R packages. The results for the studied regions in mainland Portugal suggest that the drought situations are, in general, most significant and frequent than the wet periods. From our results, we can conclude that the SPI model is more sensitive to extreme drought events and can detect them earlier. The PDSI, scPDSI and SPEI are more reliable for drought monitorization at medium and long spells, which might represent the environmental interactions more closely to the reality. Also, the scPDSI tends to reduce the importance of short period recovering. It is then advisable that impact and scientifical studies consider all of these indices or at least some of them to have a broader and complete understanding of the drought situations to be studied.
Preparation Methods and Performance Analysis of Polyanthra-Quinone/Carbon Nanotube Composites for Capturing Carbon Dioxide
Authors: Wang Zhongcheng , Feng Jingsong , Liu Xiaoyu , Guo Hao
Published: March 2022

Carbon capture is one of the important methods to achieve carbon neutrality. In this paper, a simple and reliable method for the preparation of poly(anthraquinone)/multi-walled carbon nanotube composites (PAQ/MWCNTs) for capturing carbon dioxide is proposed. Using constant magnetic stirring, 1,4-anthraquinone (1,4-AQ) was allowed to accumulate on multi-walled carbon nanotube (MWCNTs) substrates via π–π. The poly(anthraquinone)/multi-walled carbon nanotube composites were produced by this continuous process. Besides, the carbon cloth electrode prepared from PAQ/MWCNTs composites was subjected to redox potentials for carbon dioxide capture. Results showed that PAQ/MWCNTs composites had good redox reversibility, their carbon dioxide capture capacity was 7.80 mmol·g−1 while the material utilization rate reaches reached 73.4%.
ADASYN-LOF Algorithm for Imbalanced Tornado Samples
Authors: Qing Zhipeng , Zeng Qiangyu , Wang Hao , Liu Yin , Xiong Taisong , Zhang Shihao
Published: March 2022

Early warning and forecasting of tornadoes began to combine artificial intelligence (AI) and machine learning (ML) algorithms to improve identification efficiency in the past few years. Applying machine learning algorithms to detect tornadoes usually encounters class imbalance problems because tornadoes are rare events in weather processes. The ADASYN-LOF algorithm (ALA) was proposed to solve the imbalance problem of tornado sample sets based on radar data. The adaptive synthetic (ADASYN) sampling algorithm is used to solve the imbalance problem by increasing the number of minority class samples, combined with the local outlier factor (LOF) algorithm to denoise the synthetic samples. The performance of the ALA algorithm is tested by using the supporting vector machine (SVM), artificial neural network (ANN), and random forest (RF) models. The results show that the ALA algorithm can improve the performance and noise immunity of the models, significantly increase the tornado recognition rate, and have the potential to increase the early tornado warning time. ALA is more effective in preprocessing imbalanced data of SVM and ANN, compared with ADASYN, Synthetic Minority Oversampling Technique (SMOTE), SMOTE-LOF algorithms.
Behavior of Selenium during Chemical-Looping Gasification of Coal Using Copper-Based Oxygen Carrier
Authors: Ma Jingjing , Hu Jiameng , Kang Huifen , Han Ziheng , Guo Qingjie
Published: March 2022

The migration and transformation behavior of selenium during coal chemical looping gasification (CLG) under the impact of a CuO/Bentonite (Ben) oxygen carrier (OC) were studied in a batch fluidized bed reactor. In the CLG process, the total percentage of selenium released in gaseous phase was 73.06%. In the conventional gasification process, 91.71% of the total selenium was released in a gaseous state. The addition of CuO/Ben OC apparently promoted the transformation from gaseous selenium to particulate selenium. The oxygen–carbon ratio (O/C) played an important role in affecting the fraction of gaseous selenium released in the gasification process, with results showing that the amount of selenium adsorbed by CuO/Ben OC was added along with the increase in OC. By means of X-ray photoelectron spectroscopy (XPS) characterization, we found that the reduced CuO/Ben OC contained a small amount of Cu2Se due to the oxidation and adsorption of selenium onto their porous surface. The regeneration performance of the CuO/Ben OC was favorable after 10 regeneration cycles of the CLG process. The increase in the pore volumes and specific surface areas contributed to the enhanced capacity of retaining selenium for CuO/Ben OC.
Smart-Data-Driven System for Alzheimer Disease Detection through Electroencephalographic Signals
Authors: Araújo Teresa , Teixeira João Paulo , Rodrigues Pedro Miguel
Published: March 2022

Background: Alzheimer’s Disease (AD) stands out as one of the main causes of dementia worldwide and it represents around 65% of all dementia cases, affecting mainly elderly people. AD is composed of three evolutionary stages: Mild Cognitive Impairment (MCI), Mild and Moderate AD (ADM) and Advanced AD (ADA). It is crucial to create a tool for assisting AD diagnosis in its early stages with the aim of halting the disease progression. Methods: The main purpose of this study is to develop a system with the ability of differentiate each disease stage by means of Electroencephalographic Signals (EEG). Thereby, an EEG nonlinear multi-band analysis by Wavelet Packet was performed enabling to extract several features from each study group. Classic Machine Learning (ML) and Deep Learning (DL) methods have been used for data classification per EEG channel. Results: The maximum accuracies obtained were 78.9% (Healthy controls (C) vs. MCI), 81.0% (C vs. ADM), 84.2% (C vs. ADA), 88.9% (MCI vs. ADM), 93.8% (MCI vs. ADA), 77.8% (ADM vs. ADA) and 56.8% (All vs. All). Conclusions: The proposed method outperforms previous studies with the same database by 2% in binary comparison MCI vs. ADM and central and parietal brain regions revealed abnormal activity as AD progresses.
Microbiome Analysis via OTU and ASV-Based Pipelines—A Comparative Interpretation of Ecological Data in WWTP Systems
Authors: Jeske Jan Torsten , Gallert Claudia
Published: March 2022

Linking community composition and ecosystem function via the cultivation-independent analysis of marker genes, e.g., the 16S rRNA gene, is a staple of microbial ecology and dependent disciplines. The certainty of results, independent of the bioinformatic handling, is imperative for any advances made within the field. In this work, thermophilic anaerobic co-digestion experimental data, together with primary and waste-activated sludge prokaryotic community data, were analyzed with two pipelines that apply different principles when dealing with technical, sequencing, and PCR biases. One pipeline (VSEARCH) employs clustering methods, generating individual operational taxonomic units (OTUs), while the other (DADA2) is based on sequencing error correction algorithms and generates exact amplicon sequence variants (ASVs). The outcomes of both pipelines were compared within the framework of ecological-driven data analysis. Both pipelines provided comparable results that would generally allow for the same interpretations. Yet, the two approaches also delivered community compositions that differed between 6.75% and 10.81% between pipelines. Inconsistencies were also observed linked to biologically driven variability in the samples, which affected the two pipelines differently. These pipeline-dependent differences in taxonomic assignment could lead to different conclusions and interfere with any downstream analysis made for such mis- or not-identified species, e.g., network analysis or predictions of their respective ecosystem service.
Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering Applications
Authors: Matveeva Vera G. , Senokosova Evgenia A. , Sevostianova Viktoriia V. , Khanova Mariam Yu. , Glushkova Tatiana V. , Akentieva Tatiana N. , Antonova Larisa V. , Barbarash Leonid S.
Published: March 2022

Fibrin is widely used in vascular tissue engineering. Typically, fibrin polymerization is initiated by adding exogenous thrombin. In this study, we proposed a protocol for the preparation of completely autologous fibrin without the use of endogenous thrombin and compared the properties of the prepared fibrin matrix with that obtained by the traditional method. Fibrinogen was obtained by ethanol precipitation followed by fibrin polymerization by adding either exogenous thrombin and calcium chloride (ExThr), or only calcium chloride (EnThr). We examined the structure, mechanical properties, thrombogenicity, degradation rate and cytocompatibility of fibrin matrices. Factor XIII (FXIII) quantitative assay was performed by ELISA, and FXIII activity was assessed by SDS-PAGE detection of γ-γ cross-links. The results show that network structure of EnThr fibrin was characterized by thinner fibers. The EnThr fibrin matrices had higher strength, stiffness and resistance to proteolytic degradation compared to ExThr fibrin. EnThr fibrin matrices exhibited less thrombogenicity in vitro than ExThr, and retained high cytocompatibility. Thus, the proposed approach has several advantages over the traditional method, namely the fabrication of a completely autologous coating material that has better mechanical properties, higher resistance to proteolysis and lower thrombogenicity.
Triazole Modified Tetraiodothyroacetic Acid Conjugated to Polyethylene Glycol, a Thyrointegrin αvβ3 Antagonist as a Radio- and Chemo-Sensitizer in Pancreatic Cancer
Authors: Sudha Thangirala , Godugu Kavitha , Glinsky Gennadi V. , Mousa Shaker A.
Published: March 2022

Thyroid hormone L thyroxine stimulates pancreatic carcinoma cell proliferation via thyrointegrin αvβ3 receptors, and antagonist tetraiodothyroacetic acid (tetrac) inhibits cancer cell growth. Chemically modified bis-triazole-tetrac conjugated with polyethylene glycol (P-bi-TAT) has higher binding affinity to αvβ3 receptors compared to tetrac. We investigated the antiproliferation effect of P-bi-TAT in pancreatic cancer cells (SUIT2) and its radio- and chemo-sensitizing roles in a mouse model of pancreatic cancer. P-bi-TAT treatment increased tumor-targeted radiation-induced cell death and decreased tumor size. P-bi-TAT acted as a chemo-sensitizer and enhanced the 5-fluorouracil (5FU) effect in decreasing pancreatic tumor weight compared to 5FU monotherapy. Withdrawal of treatment continued the tumor regression; however, the 5FU group showed tumor regrowth. The mechanisms of the anti-cancer activity of P-bi-TAT on SUIT2 cells were assessed by microarray experiments, and genome-wide profiling identified significant alterations of 1348 genes’ expression. Both down-regulated and up-regulated transcripts suggest that a molecular interference at the signaling pathway-associated gene expression is the prevalent mode of P-bi-TAT anti-cancer activity. Our data indicate that non-cytotoxic P-bi-TAT is not only an anti-cancer agent but also a radio-sensitizer and chemo-sensitizer that acts on the extracellular domain of the cell surface αvβ3 receptor.
Effects of Transcranial Direct Current Stimulation of Bilateral Supplementary Motor Area on the Lower Limb Motor Function in a Stroke Patient with Severe Motor Paralysis: A Case Study
Authors: Ohnishi Sora , Mizuta Naomichi , Hasui Naruhito , Taguchi Junji , Nakatani Tomoki , Morioka Shu
Published: March 2022

In patients with severe motor paralysis, increasing the excitability of the supplementary motor area (SMA) in the non-injured hemisphere contributes to the recovery of lower limb motor function. However, the contribution of transcranial direct current stimulation (tDCS) over the SMA of the non-injured hemisphere in the recovery of lower limb motor function is unclear. This study aimed to examine the effects of tDCS on bilateral hemispheric SMA combined with assisted gait training. A post-stroke patient with severe motor paralysis participated in a retrospective AB design. Assisted gait training was performed only in period A and tDCS to the SMA of the bilateral hemisphere combined with assisted gait training (bi-tDCS) was performed in period B. Additionally, three conditions were performed for 20 min each in the intervals between the two periods: (1) assisted gait training only, (2) assisted gait training combined with tDCS to the SMA of the injured hemisphere, and (3) bi-tDCS. Measurements were muscle activity and beta-band intermuscular coherence (reflecting corticospinal tract excitability) of the vastus medialis muscle. The bi-tDCS immediately and longitudinally increased muscle activity and intermuscular coherence. We consider that bi-tDCS may be effective in recovering lower limb motor function in a patient with severe motor paralysis.