bluuebunny/crossref_metadata_2025 huggingface dataset:
Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy
Authors: Kawauchi Keiko , Urano Ryoto , Kinoshita Natsuki , Kuwamoto Shin , Torii Takeru , Hashimoto Yoshiki , Taniguchi Shinya , Tsuruta Mitsuki , Miyoshi Daisuke
Published: November 2020

G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).
Ancient DNA Studies in Pre-Columbian Mesoamerica
Authors: Roca-Rada Xavier , Souilmi Yassine , Teixeira João C. , Llamas Bastien
Published: November 2020

Mesoamerica is a historically and culturally defined geographic area comprising current central and south Mexico, Belize, Guatemala, El Salvador, and border regions of Honduras, western Nicaragua, and northwestern Costa Rica. The permanent settling of Mesoamerica was accompanied by the development of agriculture and pottery manufacturing (2500 BCE–150 CE), which led to the rise of several cultures connected by commerce and farming. Hence, Mesoamericans probably carried an invaluable genetic diversity partly lost during the Spanish conquest and the subsequent colonial period. Mesoamerican ancient DNA (aDNA) research has mainly focused on the study of mitochondrial DNA in the Basin of Mexico and the Yucatán Peninsula and its nearby territories, particularly during the Postclassic period (900–1519 CE). Despite limitations associated with the poor preservation of samples in tropical areas, recent methodological improvements pave the way for a deeper analysis of Mesoamerica. Here, we review how aDNA research has helped discern population dynamics patterns in the pre-Columbian Mesoamerican context, how it supports archaeological, linguistic, and anthropological conclusions, and finally, how it offers new working hypotheses.
Experimental Reproducibility and Natural Variability of Hydraulic Transport Properties of Fractured Sandstone Samples
Authors: Frank Sascha , Heinze Thomas , Ribbers Mona , Wohnlich Stefan
Published: November 2020

Flow and transport processes in fractured systems are not yet fully understood, and it is challenging to determine the respective parameters experimentally. Studies on 10 samples of 2 different sandstones were used to evaluate the reproducibility of tracer tests and the calculation of hydraulic transport properties under identical boundary conditions. The transport parameters were determined using the advection–dispersion equation (ADE) and the continuous time random walk (CTRW) method. In addition, the fracture surface morphology and the effective fracture aperture width was quantified. The hydraulic parameters and their variations were studied for samples within one rock type and between both rock types to quantify the natural variability of transport parameters as well as their experimental reproducibility. Transport processes dominated by the influence of fracture surface morphology experienced a larger spread in the determined transport parameters between repeated measurements. Grain size, effective hydraulic aperture and dispersivity were identified as the most important parameters to evaluate this effect, as with increasing fracture aperture the effect of surface roughness vanishes and the experimental reproducibility increases. Increasing roughness is often associated with the larger effective hydraulic aperture canceling out the expected increased influence of the fracture surface morphology.
The Medical Referral Process and Motor-Vehicle Crash Risk for Drivers with Dementia
Authors: Davis Jonathan , Hamann Cara , Butcher Brandon , Peek-Asa Corinne
Published: November 2020

Cognitive and physical impairment can occur with dementia and reduce driving ability. In the United States, individual states have procedures to refer and evaluate drivers who may no longer be fit to drive. The license review process is not well understood for drivers with dementia. This study uses comprehensive data from the Iowa Department of Transportation to compare the referral process for drivers with and without dementia from January 2014 through November 2019. The likelihood of failing an evaluation test was compared between drivers with and without dementia using logistic regression. The risk of motor-vehicle crash after referral for review of driving ability was compared using a Cox proportional hazard model. Analysis controlled for the age and sex of the referred driver. Drivers with dementia performed worse on all tests evaluated except the visual screening test. After the referral process, the risk of crash was similar between those with and without dementia. Drivers with dementia were denied their license more frequently than referred drivers without dementia. However, drivers with dementia who successfully kept their license as a result of the license review process were not at an increased risk of crash compared to other referred drivers.
Resurgents Create a Moral Landscape: Indigenous Resurgence and Everyday Practices of Farming in Okinawa
Authors: Chibana Megumi
Published: November 2020

Located at the territorial border of powerful states in the world, Okinawa has been a politically contested place because of the long and disproportionate hosting of the US military installations in Japan. Historically, the effects of military occupation and control of land appeared in the dispossession of Indigenous land, a transition of the local economy, and furthermore, environmental destruction of agrarian space. This essay examines everyday acts of Okinawans making Indigenous space and making the land a more livable place, despite having long been dominated and militarily occupied. More specifically, this essay explores the correlation between land-based practices of farming and (a)political activism in the community. Drawing upon ethnographic research in Okinawa, I share various stories of people engaged in active Indigenous resurgence, whom I have termed “resurgents.” Stories of these resurgents show their commitment to the land-based farming and community-based activism of restoring the Indigenous landscape and foodways. I argue that the everyday act of farming, while perhaps seemingly apolitical and personal, has been and becomes a form of sociopolitical action that not only acts to resist settler-military space but also to sustain firmly and to call forth resurgent Okinawan Indigeneity from the ground.
Impact of Internet Use on Elderly Health: Empirical Study Based on Chinese General Social Survey (CGSS) Data
Authors: Wang Jing , Liang Changyong , Li Keqing
Published: November 2020

In the current era, the rapid spread of Internet technology has combined with various traditional industries; this provides new research perspectives and solutions for current problems, such as those in the elderly care industry. Elderly health is an important social problem in various countries, and governments have turned to the internet for new methods and better solutions. However, internet-use behavior has a certain influence on the elderly’s health status. This study investigates the effects of internet use on the elderly’s physical health, mental health, and self-rated health, along with the moderating role of individual cognitive ability in the above relationship. This study uses data from the Chinese General Social Survey (CGSS) in 2012 and 2015 as samples for analysis via the hierarchical regression method. The sample is from China and had 2821 and 3185 valid respondents in 2012 and 2015, respectively. Results show that internet use significantly affects the physical and mental health of the elderly and does not significantly affect self-rated health. In addition, individual cognitive ability plays a negative moderating role between internet use and physical and mental health. Finally, on the basis of results analysis and discussion, this study provides new recommendations to achieve targeted health improvements.
Controllable H2 Generation by Formic Acid Decomposition on a Novel Pd/Templated Carbon Catalyst
Authors: Mihet Maria , Dan Monica , Barbu-Tudoran Lucian , Lazar Mihaela D. , Blanita Gabriela
Published: November 2020

A novel Pd/templated carbon catalyst (Pd/TC) was developed, characterized, and tested in the dehydrogenation of formic acid (FA) under mild conditions, with the possibility to control the H2 generation rate, in the absence or presence of HCOONa (SF), by adjusting the Pd:FA and/or FA:SF ratios. The characterization results of the templated carbon obtained by the chemical vapor deposition of acetylene on NaY zeolite revealed different structural and morphological properties compared to other C-based supports. Therefore, it was expected to induce a different catalytic behavior for the Pd/TC catalyst. Indeed, the TC-supported Pd catalyst exhibited superior activity in the decomposition of FA, even at room temperature, with turnover frequencies (TOFs) of up to 143.7 and 218.8 h−1 at 60 °C. The H2 generation rate increased with an increasing temperature, while the H2 yield increased with a decreasing FA concentration. Constant generation of gaseous flow (H2 + CO2) was achieved for 11 days, by the complete dehydrogenation of FA at room temperature using a 2 M FA solution and Pd:FA = 1:2100. The presence of SF in the reaction medium significantly enhanced the H2 generation rate (535 h−1 for FA:SF = 3:1 and 60 °C).
Impact of Coastal Wetland Restoration Plan on the Water Balance Components of Heeia Watershed, Hawaii
Authors: Ghazal Kariem A. , Leta Olkeba Tolessa , El-Kadi Aly I. , Dulai Henrietta
Published: November 2020

Optimal restoration and management of coastal wetland are contingent on reliable assessment of hydrological processes. In this study, we used the Soil and Water Assessment Tool (SWAT) model to assess the impacts of a proposed coastal wetland restoration plan on the water balance components of the Heeia watershed (Hawaii). There is a need to optimize between water needs for taro cultivation and accompanying cultural practices, wetland ecosystem services, and streamflow that feeds downstream coastal fishponds and reefs of the Heeia watershed. For this, we completed two land use change scenarios (conversion of an existing California grassland to a proposed taro field and mangroves to a pond in the wetland area) with several irrigation water diversion scenarios at different percent of minimum streamflow values in the reach. The irrigation water diversion scenarios aimed at achieving sustainable growth of the taro crop without compromising streamflow value, which plays a vital role in the health of a downstream fishpond and coastal environment of the watershed. Findings generally suggest that the conversion of a California grassland to a patched taro field is expected to decrease the baseflow value, which was a major source of streamflow for the study area, due to soil layer compaction, and thus decrease in groundwater recharge from the taro field. However, various taro irrigation water application and management scenarios suggested that diverting 50% of the minimum streamflow value for taro field would provide sustainable growth of taro crop without compromising streamflow value and environmental health of the coastal wetland and downstream fishponds.
Early Physical Rehabilitation after Sentinel Lymph Node Biopsy in Breast Cancer: Is It Feasible and Safe?
Authors: Ostos-Díaz Beatriz , Casuso-Holgado María Jesús , Muñoz-Fernández María Jesús , Carazo Ana F. , Martín-Valero Rocío , Medrano-Sánchez Esther M.
Published: November 2020

The primary purpose of this research was to investigate the feasibility and safety of delivering an early supervised physical therapy intervention to women after sentinel lymph node biopsy (SLNB); furthermore, we aimed to provide explorative data on its effects. This was a single-site feasibility study. Pre- and post-evaluation was conducted from baseline to follow-up at 6 months. Primary outcomes were participant recruitment, participant retention, compliance with the intervention, and safety. Secondary outcomes were shoulder range of motion, handgrip strength, upper limb pain and disability, scar recovery, quality of life, and the incidence of axillary web syndrome (AWS) and/or lymphoedema. A total of 43 participants (mean age 55.37 years) completed the trial and the follow-up period. A total of 91% of women who met the inclusion criteria agreed to participate, and the adherence rate was 80%. No adverse events were reported. Incidence of AWS was 9.3%, and there was no incidence of lymphoedema at 6 months. Our results support that this intervention is feasible and safe. The results presented in this study also provide preliminary evidence for the use of a rehabilitation program as a supportive intervention after SLNB, but future research on effectiveness is needed.
The Relationship between Health Status and Social Activity of Perimenopausal and Postmenopausal Women (Health Status and Social Relationships in Menopause)
Authors: Naworska Beata , Brzęk Anna , Bąk-Sosnowska Monika
Published: November 2020

The quantity and quality of interpersonal relations (including participation in University of the Third Age—U3A) play an important role for women during menopausal changes. Women who have a social network are found to be more positive about menopause, and are less likely to be depressed. This case-control study aimed to analyze the relationship between participating in formal social groups and health status related to physical activity and climacteric and depressive symptoms. The study was conducted among 621 peri- and postmenopausal women aged 50–64 years. The women were classified into two groups: U3A and controls. The participants were selected using a multistage sampling method. The IPAQ (The International Physical Activity Questionnaire), Kupperman Index (KI), and Beck Depression Inventory were used for data collection. Significant differences between the groups were confirmed in the area of professional work (p < 0.001), free time (p < 0.001), and sitting (p < 0.05). The average KI score in the U3A group was higher (t-Student = 2.12, p < 0.05). Depressive symptoms were found in 43.49% of U3A women vs. 51.15% in controls (p < 0.01). We conclude that participation in formal social groups is associated with higher level of physical activity and reduced severity of both climacteric and depressive symptoms.
Establishing a Community Air Monitoring Network in a Wildfire Smoke-Prone Rural Community: The Motivations, Experiences, Challenges, and Ideas of Clean Air Methow’s Clean Air Ambassadors
Authors: Durkin Amanda , Gonzalez Rico , Isaksen Tania Busch , Walker Elizabeth , Errett Nicole A.
Published: November 2020

In response to wildfire-related air quality issues as well as those associated with winter wood stove use and prescribed and agricultural burning, Clean Air Methow’s Clean Air Ambassador program established a community air monitoring network (CAMN) to provide geospatially specific air quality information and supplement data generated by the two Washington State Department of Ecology nephelometers situated in the area. Clean Air Ambassadors (CAAs) were purposefully selected to host low-cost air sensors based on their geographic location and interest in air quality. All 18 CAAs were interviewed to understand their motivations for participation, experiences using the data, challenges encountered, and recommendations for future project directions. Interview transcripts were coded, and a qualitative analysis approach was used to identify the key themes in each domain. The reported motivations for participation as a CAA included reducing personal exposure, protecting sensitive populations, interest in air quality or environmental science, and providing community benefits. CAAs used CAMN data to understand air quality conditions, minimize personal or familial exposure, and engage other community members in air quality discussions. Opportunities for future project directions included use for monitoring other seasonal air quality issues, informing or reducing other pollution-generating activities, school and community educational activities, opportunities for use by and engagement of different stakeholder groups, and mobile-friendly access to CAMN information. Limited challenges associated with participation were reported. Additional research is necessary to understand the community-level impacts of the CAMN. The findings may be informative for other rural wildfire smoke-prone communities establishing similar CAMNs.
Developing an Ensemble Predictive Safety Risk Assessment Model: Case of Malaysian Construction Projects
Authors: Sadeghi Haleh , Mohandes Saeed Reza , Hosseini M. Reza , Banihashemi Saeed , Mahdiyar Amir , Abdullah Arham
Published: November 2020

Occupational Health and Safety (OHS)-related injuries are vexing problems for construction projects in developing countries, mostly due to poor managerial-, governmental-, and technical safety-related issues. Though some studies have been conducted on OHS-associated issues in developing countries, research on this topic remains scarce. A review of the literature shows that presenting a predictive assessment framework through machine learning techniques can add much to the field. As for Malaysia, despite the ongoing growth of the construction sector, there has not been any study focused on OHS assessment of workers involved in construction activities. To fill these gaps, an Ensemble Predictive Safety Risk Assessment Model (EPSRAM) is developed in this paper as an effective tool to assess the OHS risks related to workers on construction sites. The developed EPSRAM is based on the integration of neural networks with fuzzy inference systems. To show the effectiveness of the EPSRAM developed, it is applied to several Malaysian construction case projects. This paper contributes to the field in several ways, through: (1) identifying major potential safety risks, (2) determining crucial factors that affect the safety assessment for construction workers, (3) predicting the magnitude of identified safety risks accurately, and (4) predicting the evaluation strategies applicable to the identified risks. It is demonstrated how EPSRAM can provide safety professionals and inspectors concerned with well-being of workers with valuable information, leading to improving the working environment of construction crew members.
Environmental Characteristics Associated with Older Adults’ Social Participation: The Contribution of Sociodemography and Transportation in Metropolitan, Urban, and Rural Areas
Authors: Levasseur Mélanie , Naud Daniel , Bruneau Jean-François , Généreux Mélissa
Published: November 2020

Although social participation fosters older adults’ health, little is known about which environmental characteristics are related to greater participation in social activities. The Canadian Community Health Survey (n = 2737), a transportation survey, and multiple secondary data sources were used to identify the environmental characteristics associated with older Quebecers’ social participation according to living area. Greater social participation was associated with: (1) a higher concentration of older adults (IRR = 2.172 (95% CI 1.600, 2.948); p < 0.001), more kilometers traveled by paratransit (IRR = 1.714 (95% CI 1.286, 2.285); p < 0.01), a lack of medical clinics (IRR = 0.730 (95% CI 0.574, 0.930); p = 0.01), and more funded home adaptations (IRR = 1.170 (95% CI 1.036, 1.320); p = 0.01) in large metropolitan areas; (2) larger paratransit fleets (IRR = 1.368 (95% CI 1.044, 1.791); p = 0.02) and a lower density of road intersections (IRR = 0.862 (95% CI 0.756, 0.982); p = 0.03) in regular metropolitan areas; (3) less social deprivation (IRR = 1.162 (95% CI 1.025, 1.318); p = 0.02) in urban areas; and (4) a higher concentration of older populations (IRR = 2.386 (95% CI 1.817, 3.133); p < 0.001) in rural areas. According to these findings, social participation interventions should target the local environment—for example, by providing more social interaction opportunities for older adults living in younger neighborhoods and by improving access to public transportation, especially paratransit.
Are Maternal Feeding Practices and Mealtime Emotions Associated with Toddlers’ Food Neophobia? A Follow-Up to the DIT-Coombe Hospital Birth Cohort in Ireland
Authors: An Meijing , Zhou Qianling , Younger Katherine M. , Liu Xiyao , Kearney John M.
Published: November 2020

This study was conducted to explore the associations between maternal feeding practices, mealtime emotions, as well as maternal food neophobia and toddlers’ food neophobia in Ireland. A follow-up to the Technological University Dublin (DIT)-Coombe Hospital birth cohort was conducted. Mothers in the original cohort were invited to the present study by telephone calls. Postal questionnaires with stamped addressed envelopes were distributed to those who agreed to participate in the study. Toddler food neophobia was assessed by the modified version of the Child Food Neophobia Scale (CFNS). There were 205 participants included in this study, with a median score of child food neophobia of 12. A higher degree of child food neophobia (score > 12) was positively associated with the maternal practice of coaxing the children to eat at refusal (OR (Odds Ratio) = 2.279, 95% CI: 1.048–4.955), unpleasant emotions at mealtime (e.g., stressful or hectic for mothers, or tearful for children) (OR ranged between 1.618 and 1.952), and mothers’ own degree of food neophobia (OR = 1.036, 95% CI: 1.001–1.072). Mothers who were not worried when confronted with child’s food refusal was negatively associated with toddlers’ food neophobia (OR = 0.251, 95% CI: 0.114–0.556). This study suggests the maternal practices of responsive feeding, being calm and patient with the toddlers, and creating a positive atmosphere at mealtime.
Interpersonal Psychotherapy to Reduce Psychological Distress in Perinatal Women: A Systematic Review
Authors: Bright Katherine S. , Charrois Elyse M. , Mughal Muhammad Kashif , Wajid Abdul , McNeil Deborah , Stuart Scott , Hayden K. Alix , Kingston Dawn
Published: November 2020

Background: Interpersonal psychotherapy (IPT) is a psychological intervention with established efficacy in the prevention and treatment of depressive disorders. Previous systematic reviews have not evaluated the effectiveness of IPT on symptoms of stress, anxiety, depression, quality of life, relationship satisfaction/quality, social supports, and an improved psychological sense of wellbeing. There is limited information regarding moderating and mediating factors that impact the effectiveness of IPT such as the timing of the intervention or the mode of delivery of IPT intervention. The overall objective of this systematic review was to evaluate the effectiveness of IPT interventions to treat perinatal (from pregnancy up to 12 months postpartum) psychological distress. Methods: MEDLINE(R) and Epub Ahead of Print, In-Process & Other Non-Indexed Citations and Daily (Ovid), EMBASE (Ovid), PsycINFO (Ovid), Cochrane Central Register of Controlled Trials (OVID), CINAHL with Full Text (Ebsco), Social Work Abstracts (Ebsco), SocINDEX with Full Text (Ebsco), Academic Search Complete (Ebsco), Family & Society Studies Worldwide (Ebsco), Family Studies Abstracts (Ebsco), and Scopus databases were searched from inception until 31 January 2019. Two researchers independently screened articles for eligibility. Of the 685 screened articles, 43 met the inclusion criteria. The search was re-run on 11 May 2020. An additional 204 articles were screened and two met the inclusion criteria, resulting in a total of 45 studies included in this review. There were 25 Randomized Controlled Trials, 10 Quasi-experimental studies, eight Open Trials, and two Single Case Studies. All included studies were critically appraised for quality. Results: In most studies (n = 24, 53%), the IPT intervention was delivered individually; in 17 (38%) studies IPT was delivered in a group setting and two (4%) studies delivered the intervention as a combination of group and individual IPT. Most interventions were initiated during pregnancy (n = 27, 60%), with the remaining 18 (40%) studies initiating interventions during the postpartum period. Limitations: This review included only English-language articles and peer-reviewed literature. It excluded government reports, dissertations, conference papers, and reviews. This limited the access to grassroots or community-based recruitment and retention strategies that may have been used to target smaller or marginalized groups of perinatal women. Conclusions: IPT is an effective intervention for the prevention and treatment of psychological distress in women during their pregnancy and postpartum period. As a treatment intervention, IPT is effective in significantly reducing symptoms of depression and anxiety as well as improving social support, relationship quality/satisfaction, and adjustment. Systematic Review Registration: PROSPERO CRD42019114292.
Fractal-Based Modeling and Spatial Analysis of Urban Form and Growth: A Case Study of Shenzhen in China
Authors: Man Xiaoming , Chen Yanguang
Published: November 2020

Fractal dimension curves of urban growth can be modeled with sigmoid functions, including logistic function and quadratic logistic function. Different types of logistic functions indicate different spatial dynamics. The fractal dimension curves of urban growth in Western countries follow the common logistic function, while the fractal dimension growth curves of cities in northern China follow the quadratic logistic function. Now, we want to investigate whether other Chinese cities, especially cities in South China, follow the same rules of urban evolution and attempt to analyze the reasons. This paper is devoted to exploring the fractals and fractal dimension properties of the city of Shenzhen in southern China. The urban region is divided into four subareas using ArcGIS technology, the box-counting method is adopted to extract spatial datasets, and the least squares regression method is employed to estimate fractal parameters. The results show that (1) the urban form of Shenzhen city has a clear fractal structure, but fractal dimension values of different subareas are different; (2) the fractal dimension growth curves of all the four study areas can only be modeled by the common logistic function, and the goodness of fit increases over time; (3) the peak of urban growth in Shenzhen had passed before 1986 and the fractal dimension growth is approaching its maximum capacity. It can be concluded that the urban form of Shenzhen bears characteristics of multifractals and the fractal structure has been becoming better, gradually, through self-organization, but its land resources are reaching the limits of growth. The fractal dimension curves of Shenzhen’s urban growth are similar to those of European and American cities but differ from those of cities in northern China. This suggests that there are subtle different dynamic mechanisms of city development between northern and southern China.
Time-Series Clustering for Home Dwell Time during COVID-19: What Can We Learn from It?
Authors: Huang Xiao , Li Zhenlong , Lu Junyu , Wang Sicheng , Wei Hanxue , Chen Baixu
Published: November 2020

In this study, we investigate the potential driving factors that lead to the disparity in the time-series of home dwell time in a data-driven manner, aiming to provide fundamental knowledge that benefits policy-making for better mitigation strategies of future pandemics. Taking Metro Atlanta as a study case, we perform a trend-driven analysis by conducting Kmeans time-series clustering using fine-grained home dwell time records from SafeGraph. Furthermore, we apply ANOVA (Analysis of Variance) coupled with post-hoc Tukey’s test to assess the statistical difference in sixteen recoded demographic/socioeconomic variables (from ACS 2014–2018 estimates) among the identified time-series clusters. We find that demographic/socioeconomic variables can explain the disparity in home dwell time in response to the stay-at-home order, which potentially leads to disparate exposures to the risk from the COVID-19. The results further suggest that socially disadvantaged groups are less likely to follow the order to stay at home, pointing out the extensive gaps in the effectiveness of social distancing measures that exist between socially disadvantaged groups and others. Our study reveals that the long-standing inequity issue in the U.S. stands in the way of the effective implementation of social distancing measures.
Forecasting of Short-Term Daily Tourist Flow Based on Seasonal Clustering Method and PSO-LSSVM
Authors: Li Keqing , Liang Changyong , Lu Wenxing , Li Chu , Zhao Shuping , Wang Binyou
Published: November 2020

The accurate prediction of tourist flow is essential to appropriately prepare tourist attractions and inform the decisions of tourism companies. However, tourist flow in scenic spots is a dynamic trend with daily changes, and specialized methods are necessary to measure it accurately. For this purpose, a tourist flow forecasting method is proposed in this research based on seasonal clustering. The experiment employs the K-means algorithm considering seasonal variations and the particle swarm optimization-least squares support vector machine (PSO-LSSVM) algorithm to forecast the tourist flow in scenic spots. The LSSVM is also used to compare the performance of the proposed model with that of the existing ones. Experiments based on a dataset comprising the daily tourist data for Mountain Huangshan during the period between 2014 and 2017 are conducted. Our results show that seasonal clustering is an effective method to improve tourist flow prediction, besides, the accuracy of daily tourist flow prediction is significantly improved by nearly 3 percent based on the hybrid optimized model combining seasonal clustering. Compared with other algorithms which provide predictions at monthly intervals, the method proposed in this research can provide more timely analysis and guide professionals in the tourism industry towards better daily management.
The Role of Neutrophils in Hypertension
Authors: Araos Patricio , Figueroa Stefanny , Amador Cristián A.
Published: November 2020

It is well accepted that the immune system and some cells from adaptive and innate immunity are necessary for the initiation/perpetuation of arterial hypertension (AH). However, whether neutrophils are part of this group remains debatable. There is evidence showing that the neutrophil/lymphocyte ratio correlates with AH and is higher in non-dipper patients. On the other hand, the experimental neutrophil depletion in mice reduces basal blood pressure. Nevertheless, their participation in AH is still controversial. Apparently, neutrophils may modulate the microenvironment in blood vessels by increasing oxidative stress, favoring endothelial disfunction. In addition, neutrophils may contribute to the tissue infiltration of immune cells, secreting chemoattractant chemokines/cytokines and promoting the proinflammatory phenotype, leading to AH development. In this work, we discuss the potential role of neutrophils in AH by analyzing different mechanisms proposed from clinical and basic studies, with a perspective on cardiovascular and renal damages relating to the hypertensive phenotype.
Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by Aspergillus westerdijkiae and Penicillium verrucosum
Authors: Boonmee Saranyaphat , Atanasova Vessela , Chéreau Sylvain , Marchegay Gisèle , Hyde Kevin D. , Richard-Forget Florence
Published: November 2020

Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), p-coumaric (COUM), caffeic and chlorogenic acids was evaluated on Aspergillus westerdijkiae and Penicillium verrucosum. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by P. verrucosum and, although not statistically significant, a 35% inhibition of OTA produced by A. westerdijkiae. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of P. verrucosum to metabolize FER and COUM through a C2-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides.
The Importance of Hypoxia-Inducible Factors (HIF-1 and HIF-2) for the Pathophysiology of Inflammatory Bowel Disease
Authors: Kerber Evelyn L. , Padberg Claudia , Koll Nora , Schuetzhold Vera , Fandrey Joachim , Winning Sandra
Published: November 2020

(1) Background: Hypoxia is a common feature of inflammation when hypoxia inducible factors (HIFs) adapt cells to conditions of low oxygen tension and inflammation. We studied the role of HIF-1 and HIF-2 in cells of the myeloid lineage in a mouse model of acute colitis. (2) Methods: Mice with and without a conditional knockout for either Hif-1a or Hif-2a or Hif-1a and Hif-2a in cells of the myeloid lineage were treated with 2.5% dextran sodium sulfate (DSS) for 6 days to induce an acute colitis. We analyzed the course of inflammation with respect to macroscopic (disease activity index) and microscopic (histology score and immunohistochemical staining of immune cells) parameters and quantified the mRNA expression of cytokines and chemokines in the colon and the mesenteric lymph nodes. (3) Results: A conditional knockout of myeloid Hif-1a ameliorated whereas the knockout of Hif-2a aggravated murine DSS colitis by increased recruitment of neutrophils to deeper layers of the colon. This led to higher expression of Il6, Ifng, Cd11c, Cd4, and Cd8 in the colon but also induced anti-inflammatory mediators such as Foxp3 and Il10. A conditional knockout of Hif-1a and Hif-2a did not show any differences compared to wildtype mice. (4) Conclusions: Myeloid HIF-1α and HIF-2α play opposing roles in acute DSS colitis. Thus, not only a cell type specific, but also the isoform specific modulation of HIFs needs to be addressed in attempts to modify HIF for therapeutic purposes.
Axon Injury-Induced Autophagy Activation Is Impaired in a C. elegans Model of Tauopathy
Authors: Ko Su-Hyuk , Gonzalez Gilberto , Liu Zhijie , Chen Lizhen
Published: November 2020

Autophagy is a conserved pathway that plays a key role in cell homeostasis in normal settings, as well as abnormal and stress conditions. Autophagy dysfunction is found in various neurodegenerative diseases, although it remains unclear whether autophagy impairment is a contributor or consequence of neurodegeneration. Axonal injury is an acute neuronal stress that triggers autophagic responses in an age-dependent manner. In this study, we investigate the injury-triggered autophagy response in a C. elegans model of tauopathy. We found that transgenic expression of pro-aggregant Tau, but not the anti-aggregant Tau, abolished axon injury-induced autophagy activation, resulting in a reduced axon regeneration capacity. Furthermore, axonal trafficking of autophagic vesicles were significantly reduced in the animals expressing pro-aggregant F3ΔK280 Tau, indicating that Tau aggregation impairs autophagy regulation. Importantly, the reduced number of total or trafficking autophagic vesicles in the tauopathy model was not restored by the autophagy activator rapamycin. Loss of PTL-1, the sole Tau homologue in C. elegans, also led to impaired injury-induced autophagy activation, but with an increased basal level of autophagic vesicles. Therefore, we have demonstrated that Tau aggregation as well as Tau depletion both lead to disruption of injury-induced autophagy responses, suggesting that aberrant protein aggregation or microtubule dysfunction can modulate autophagy regulation in neurons after injury.
Coordinated Regulation of Mesenchymal Stem Cell Migration by Various Chemotactic Stimuli
Authors: Nam Donghyun , Park Aran , Dubon Maria Jose , Yu Jinyeong , Kim Wootak , Son Youngsook , Park Ki-Sook
Published: November 2020

Endogenous bone marrow-derived mesenchymal stem cells are mobilized to peripheral blood and injured tissues in response to changes in the expression of various growth factors and cytokines in the injured tissues, including substance P (SP), transforming growth factor-beta (TGF-β), and stromal cell-derived factor-1 (SDF-1). SP, TGF-β, and SDF-1 are all known to induce the migration of bone marrow-derived mesenchymal stem cells (BM-MSCs). However, it is not yet clear how these stimuli influence or interact with each other during BM-MSC mobilization. This study used mouse bone marrow-derived mesenchymal stem cell-like ST2 cells and human BM-MSCs to evaluate whether SP, TGF-β, and SDF-1 mutually regulate their respective effects on the mobilization of BM-MSCs. SP pretreatment of ST2 and BM-MSCs impaired their response to TGF-β while the introduction of SP receptor antagonist restored the mobilization of ST2 and BM-MSCs in response to TGF-β. TGF-β pretreatment did not affect the migration of ST2 and BM-MSCs in response to SP, but downregulated their migration in response to SDF-1. SP pretreatment modulated the activation of TGF-β noncanonical pathways in ST2 cells and BM-MSCs, but not canonical pathways. These results suggest that the migration of mesenchymal stem cells is regulated by complex functional interactions between SP, TGF-β, and SDF-1. Thus, understanding the complex functional interactions of these chemotactic stimuli would contribute to ensuring the development of safe and effective combination treatments for the mobilization of BM-MSCs.
mRNA and miRNA Profiles of Exosomes from Cultured Tumor Cells Reveal Biomarkers Specific for HPV16-Positive and HPV16-Negative Head and Neck Cancer
Authors: Ludwig Sonja , Sharma Priyanka , Wise Petra , Sposto Richard , Hollingshead Deborah , Lamb Janette , Lang Stephan , Fabbri Muller , Whiteside Theresa L.
Published: November 2020

Human papillomavirus (HPV)(+) and HPV(−) head and neck cancer (HNC) cells’ interactions with the host immune system are poorly understood. Recently, we identified molecular and functional differences in exosomes produced by HPV(+) vs. HPV(−) cells, suggesting that genetic cargos of exosomes might identify novel biomarkers in HPV-related HNCs. Exosomes were isolated by size exclusion chromatography from supernatants of three HPV(+) and two HPV(−) HNC cell lines. Paired cell lysates and exosomes were analyzed for messenger RNA (mRNA) by qRT-PCR and microRNA (miR) contents by nanostring analysis. The mRNA profiles of HPV(+) vs. HPV(−) cells were distinct, with EGFR, TP53 and HSPA1A/B overexpressed in HPV(+) cells and IL6, FAS and DPP4 in HPV(−) cells. The mRNA profiles of HPV(+) or HPV(−) exosomes resembled the cargo of their parent cells. miR expression profiles in cell lysates identified 8 miRs expressed in HPV(−) cells vs. 14 miRs in HPV(+) cells. miR-205-5p was exclusively expressed in HPV(+) exosomes, and miR-1972 was only detected in HPV(−) exosomes. We showed that HPV(+) and HPV(−) exosomes recapitulated the mRNA expression profiles of their parent cells. Expression of miRs was dependent on the HPV status, and miR-205-5p in HPV(+) and miR-1972 in HPV(−) exosomes emerge as potential discriminating HPV-associated biomarkers.
Insights into Potential Targets for Therapeutic Intervention in Epilepsy
Authors: Zavala-Tecuapetla Cecilia , Cuellar-Herrera Manola , Luna-Munguia Hiram
Published: November 2020

Epilepsy is a chronic brain disease that affects approximately 65 million people worldwide. However, despite the continuous development of antiepileptic drugs, over 30% patients with epilepsy progress to drug-resistant epilepsy. For this reason, it is a high priority objective in preclinical research to find novel therapeutic targets and to develop effective drugs that prevent or reverse the molecular mechanisms underlying epilepsy progression. Among these potential therapeutic targets, we highlight currently available information involving signaling pathways (Wnt/β-catenin, Mammalian Target of Rapamycin (mTOR) signaling and zinc signaling), enzymes (carbonic anhydrase), proteins (erythropoietin, copine 6 and complement system), channels (Transient Receptor Potential Vanilloid Type 1 (TRPV1) channel) and receptors (galanin and melatonin receptors). All of them have demonstrated a certain degree of efficacy not only in controlling seizures but also in displaying neuroprotective activity and in modifying the progression of epilepsy. Although some research with these specific targets has been done in relation with epilepsy, they have not been fully explored as potential therapeutic targets that could help address the unsolved issue of drug-resistant epilepsy and develop new antiseizure therapies for the treatment of epilepsy.
Evidence of Pollinators Foraging on Centipedegrass Inflorescences
Authors: V. Joseph Shimat , Harris-Shultz Karen , Jespersen David
Published: November 2020

Turfgrasses are commonly used for lawns and as recreational surfaces in the USA. Because grasses are largely wind-pollinated, it was thought that pollinators would not forage on turfgrasses. Centipede grass (Eremochloa ophiuroides (Munro) Hack) is a warm-season turfgrass widely used in the southeastern USA. Centipede grass produces spike-like inflorescences from August to October, and little is known about whether pollinators utilize those inflorescences as pollen resources. Thus, the objective of the current study was to identify the pollinators foraging on centipede grass inflorescences. Pollinator samples were collected by (1) sweeping the insects actively foraging on centipede grass inflorescence for 30 min, (2) deploying pan traps for 24 h and (3) deploying malaise traps for 7 d. In the sweep samples, Lasioglossum spp., Bombus spp., Apis spp., Melissodes spp. and Augochlorella spp. were collected from centipede grass inflorescences. Syrphid flies were also collected in the sweep samples. The pan and malaise traps collected mostly Lasioglossum spp. The results imply that there is a critical need to conserve bee habitats and adopt nondisruptive lawn practices. Additionally, this new knowledge lays the foundation for future research to enhance our understanding of bee and syrphid behavior and the selection of host traits for improving bee foraging.
Using Moderate Transgene Expression to Improve the Genetic Sexing System of the Australian Sheep Blow Fly Lucilia cuprina
Authors: Yan Ying , Williamson Megan E. , Scott Maxwell J.
Published: November 2020

The sterile insect technique (SIT) is a promising strategy to control the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. We have previously developed a transgenic embryonic sexing system (TESS) for this pest to facilitate the potential SIT application. TESS carry two transgenes, a tetracycline transactivator (tTA) driver and a tTA-activated pro-apoptotic effector. TESS females die at the embryonic stage unless tetracycline is supplied in the diet. However, undesired female sterility was observed in some TESS strains without tetracycline due to expression of tTA in ovaries. Here we investigate if TESS that combine transgenes with relatively low/moderate expression/activity improves the fertility of TESS females. tTA driver lines were evaluated for tTA expression by quantitative real time PCR and/or by crossing with a tTA-activated RFPex effector line. Fertility and lethality tests showed that a TESS strain containing a driver line with moderate tTA expression and an effector line showing moderate pro-apoptotic activity could recover the fertility of parental females and eliminated all female offspring at the embryonic stage. Consequently, such a strain could be further evaluated for an SIT program for L. cuprina, and such a “moderate strategy” could be considered for the TESS development in other pest species.
An Evaluation of Wearable Inertial Sensor Configuration and Supervised Machine Learning Models for Automatic Punch Classification in Boxing
Authors: Worsey Matthew T. O. , Espinosa Hugo G. , Shepherd Jonathan B. , Thiel David V.
Published: November 2020

Machine learning is a powerful tool for data classification and has been used to classify movement data recorded by wearable inertial sensors in general living and sports. Inertial sensors can provide valuable biofeedback in combat sports such as boxing; however, the use of such technology has not had a global uptake. If simple inertial sensor configurations can be used to automatically classify strike type, then cumbersome tasks such as video labelling can be bypassed and the foundation for automated workload monitoring of combat sport athletes is set. This investigation evaluates the classification performance of six different supervised machine learning models (tuned and untuned) when using two simple inertial sensor configurations (configuration 1—inertial sensor worn on both wrists; configuration 2—inertial sensor worn on both wrists and third thoracic vertebrae [T3]). When trained on one athlete, strike prediction accuracy was good using both configurations (sensor configuration 1 mean overall accuracy: 0.90 ± 0.12; sensor configuration 2 mean overall accuracy: 0.87 ± 0.09). There was no significant statistical difference in prediction accuracy between both configurations and tuned and untuned models (p > 0.05). Moreover, there was no significant statistical difference in computational training time for tuned and untuned models (p > 0.05). For sensor configuration 1, a support vector machine (SVM) model with a Gaussian rbf kernel performed the best (accuracy = 0.96), for sensor configuration 2, a multi-layered perceptron neural network (MLP-NN) model performed the best (accuracy = 0.98). Wearable inertial sensors can be used to accurately classify strike-type in boxing pad work, this means that cumbersome tasks such as video and notational analysis can be bypassed. Additionally, automated workload and performance monitoring of athletes throughout training camp is possible. Future investigations will evaluate the performance of this algorithm on a greater sample size and test the influence of impact window-size on prediction accuracy. Additionally, supervised machine learning models should be trained on data collected during sparring to see if high accuracy holds in a competition setting. This can help move closer towards automatic scoring in boxing.