bluuebunny/crossref_metadata_2025 huggingface dataset:
The methods of improving cultural sensitivity of depression scales for use among global indigenous populations: a systematic scoping review
Authors: Gomez Cardona Liliana , Yang Michelle , Seon Quinta , Karia Maharshee , Velupillai Gajanan , Noel Valérie , Linnaranta Outi
Published: November 2023

Cultural adaptation of psychometric measures has become a process aimed at increasing acceptance, reliability, and validity among specific Indigenous populations. We present a systematic scoping review to: (1) identify the depression scales that have been culturally adapted for use among Indigenous populations worldwide, (2) globally report on the methods used in the cultural adaptation of those scales, and (3) describe the main features of those cultural adaptation methods. We included articles published from inception to April 2021, including 3 levels of search terms: Psychometrics, Indigenous, and Depression. The search was carried out in the Ovid Medline, PubMed, Embase, Global Health, PsycINFO, and CINAHL databases, following PRISMA guidelines. We identified 34 reports on processes of cultural adaptation that met the criteria. The scales were adapted for use among Indigenous populations from Africa, Australia, Asia, North America, and Latin America. The most common scales that underwent adaptation were the Patient Health Questionnaire (PHQ-9), the Center for Epidemiologic Studies Depression Scale (CES-D), and the Edinburgh Postnatal Depression Scale (EPDS). Methods of adaptation involved a revision of the measures’ cultural appropriateness, standard/transcultural translation, revision of the administration process, and inclusion of visual supports. Culturally safe administration of scales was reported in some studies. To come to a consensus on most appropriate methods of improving cultural safety of psychometric measurement, most studies utilized qualitative methods or mixed methods to understand the specific community’s needs. Revision of linguistic equivalence and cultural relevance of content, culturally safe administration procedures, qualitative methods, and participatory research were key features of developing safe culturally adapted measures for depressive symptoms among Indigenous populations. While for comparability, uniform scales would be ideal as mental health evaluations, an understanding of the cultural impact of measurements and local depression expressions would benefit the process of developing culturally sensitive psychometric scales. PROSPERO registration ID: CRD42023391439.
The clandestine welfare: The role of illicit actors in the provision of social protection in Latin America
Authors: Zapata Celestino Kevin
Published: November 2023

There has been an increasing interest in exploring the different types of welfare regimes in middle- and low-income countries. However, most of the studies so far seem to neglect the importance of illicit groups as welfare providers. Illicit groups can be so powerful in many of these societies, that they can create extensive social safety nets embedded in parallel governance orders. Considering their importance as fundamental actors in the Global South, it is required to acknowledge their role as welfare deliverers as a missing piece in the literature. Hence I discuss the nature of what I call “clandestine welfare” and three specific forms of social protection provided by illicit groups: direct, involuntary, and forced. In the first form, the illicit groups provide directly to society by delivering goods or services that the formal state fails to provide. In the second, the illicit group’s activities have involuntary but positive spill-overs over society in terms of welfare. In the last one, the illicit groups use violence to force other social actors to provide social protection. By bringing illustrative examples from Latin America, I aim to show that the action of illicit groups represents a tangible source of welfare for a large segment of the population that goes beyond charity.
TWO EXAMPLES CONCERNING EXISTENTIAL UNDECIDABILITY IN FIELDS
Authors: DITTMANN PHILIP
Published: November 2023

We construct an existentially undecidable complete discretely valued field of mixed characteristic with existentially decidable residue field and decidable algebraic part, answering a question by Anscombe–Fehm in a strong way. Along the way, we construct an existentially decidable field of positive characteristic with an existentially undecidable finite extension, modifying a construction due to Kesavan Thanagopal.
Evaluation of the emergency nutrition response in Malatya after Türkiye–Syria earthquake
Authors: Kalyoncu Atasoy Zeynep Begum , Kurt S. Ceren , Beckerman-Hsu Jacob , Soyunen Senanur , Kalyoncu M. Fuatcan , Kaptanoglu Aysegul
Published: November 2023

The Türkiye–Syria earthquake struck eleven provinces directly in Türkiye on 6 February 2023. Emergency nutrition care is indispensable for sustaining the lives of victims and rescue personnel. To optimally support their well-being, emergency food must be both healthy (i.e. aligned with dietary guidelines) and safe. However, globally, there is a dearth of research on the emergency nutrition conditions in shelters in the immediate aftermath of natural disasters. This lack of scientific evidence could limit the extent to which nutritional gaps can be identified and remedied for future relief efforts. Therefore, the aim of this research was to evaluate the nutrition environment and nutritional quality of emergency meals distributed to survivors in Malatya, a heavily affected province in Türkiye. The rapid assessment was conducted in thirteen locations by using an embedded case–study design to evaluate the nutrition environment both quantitatively and qualitatively. Meals served to earthquake victims and volunteers were found to be insufficient in protein, fat, fibre, vitamin C, Ca and Fe, but Na levels were higher than the maximum threshold in many of the centres. The qualitative analysis illustrated insufficiency in three domains of the emergency food and nutrition environment: foods and beverages offered, cooking/food preparation and food safety and dining areas and other facilities. Given the major nutritional gaps identified in this study, future disaster preparations should implement emergency nutrition plans that ensure healthy, nutritious and safe food for survivors. Better coordination and use of technology are necessary for interventions to prevent malnutrition.
A longitudinal study of the gut microbiota during the first three years of life: Links with problem behavior and executive functions at preschool age
Authors: Willemsen Yvonne , Ou Yangwenshan , Belzer Clara , Arias Vásquez Alejandro , Smidt Hauke , Beijers Roseriet , de Weerth Carolina
Published: November 2023

Early life is a sensitive period when microbiota-gut-brain interactions may have important impact on development. This study investigated the associations of the gut microbiota in the first three years of life (two, six, and 12 weeks, and one and three years) with problem behavior and executive functions in N = 64 three-year-old children. Higher relative abundance of Streptococcus at the age of two weeks, as well as its trajectory over time (including ages two, six and 12 weeks, and one and three years), was related to worse executive functions. Higher relative abundance of [Ruminococcus] torques group at the age of three years, as well as its trajectory from one to three years, was associated with less internalizing behavior. Besides, several robust age-specific associations were identified: higher Bifidobacterium relative abundance (age three years) was associated with more internalizing and externalizing issues; higher Blautia relative abundance (age three years) was linked to less internalizing behavior; and increased relative abundance of an unidentified Enterobacteriaceae genus (age two weeks) was related to more externalizing behavior. Our findings provide important longitudinal evidence that early-life gut microbiota may be linked to behavioral and cognitive development in low-risk children.
Cognitive behavioural therapy for depression and anxiety in a carer for two family members with dementia: a single case experimental design study
Authors: Falshaw Lawson , Clatworthy Leah
Published: November 2023

Background:

Evidence suggests that cognitive behavioural therapy (CBT) can be a helpful approach for older adults experiencing anxiety and depression. Some research has suggested this is also the case for those caring for a family member with dementia. Little research has been conducted into the impact of CBT for older adults juggling the demands of caring for multiple family members with dementia.

Aims:

This case study aimed to evaluate the application of CBT to ‘Mrs P’, a 68-year-old client experiencing anxiety and depression whilst caring for two family members with dementia.

Method:

A single case experimental design study was conducted to assess the effectiveness of CBT formulation and intervention, including cognitive restructuring of unhelpful thoughts about caregiving and increasing engagement in pleasurable activities.

Results:

Mrs P’s depression and anxiety scores improved significantly throughout treatment, and she met her goal of being able to manage when caregiving activities go wrong during daily life.

Conclusions:

CBT may be a helpful approach to reducing anxiety and depression in dementia family caregivers.
The effect of a foreign language on cognitive reappraisal during exposure to a phobic stimulus
Authors: Ortigosa Isabel , Jaén Irene , Costumero Víctor , García-Palacios Azucena
Published: November 2023

This study investigates whether the cognitive reappraisal strategy is influenced by the participant's language (native/foreign) when confronting a fearful stimulus. Sixty participants with subclinical phobia of cockroaches were exposed to several phobic and neutral pictures while they used cognitive reappraisal in their native language or a foreign one. Electrodermal activity, pupil dilation, and self-reports of affective valence and arousal were collected. Results showed that participants in the foreign context were more effective at using reappraisal to reduce valence self-ratings compared to using no regulation. Also, participants in the foreign context showed greater pupil size when reappraising their emotions, compared to the non-regulation condition. Depending on the language, no differences were found for arousal self-reports or electrodermal activity when using reappraisal. These results suggest that using a foreign language could be advantageous in reducing the negative valence through reappraisal. Psychophysiological results are discussed in light of cognitive effort studies.
Spacecraft Are Sprinkling the Stratosphere with Metal
Authors: Herring Molly
Published: November 2023

Metals from spacecraft reentry don’t simply vaporize and vanish. Scientists found them in the stratosphere.
Engineering electrode interfaces for telecom-band photodetection in MoS2/Au heterostructures via sub-band light absorption
Authors: Hong Chengyun , Oh Saejin , Dat Vu Khac , Pak Sangyeon , Cha SeungNam , Ko Kyung-Hun , Choi Gyung-Min , Low Tony , Oh Sang-Hyun , Kim Ji-Hee
Published: November 2023

Transition metal dichalcogenide (TMD) layered semiconductors possess immense potential in the design of photonic, electronic, optoelectronic, and sensor devices. However, the sub-bandgap light absorption of TMD in the range from near-infrared (NIR) to short-wavelength infrared (SWIR) is insufficient for applications beyond the bandgap limit. Herein, we report that the sub-bandgap photoresponse of MoS2/Au heterostructures can be robustly modulated by the electrode fabrication method employed. We observed up to 60% sub-bandgap absorption in the MoS2/Au heterostructure, which includes the hybridized interface, where the Au layer was applied via sputter deposition. The greatly enhanced absorption of sub-bandgap light is due to the planar cavity formed by MoS2 and Au; as such, the absorption spectrum can be tuned by altering the thickness of the MoS2 layer. Photocurrent in the SWIR wavelength range increases due to increased absorption, which means that broad wavelength detection from visible toward SWIR is possible. We also achieved rapid photoresponse (~150 µs) and high responsivity (17 mA W−1) at an excitation wavelength of 1550 nm. Our findings demonstrate a facile method for optical property modulation using metal electrode engineering and for realizing SWIR photodetection in wide-bandgap 2D materials.
MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis
Authors: Liu Shuzhen , Perez Preston , Sun Xue , Chen Ken , Fatirkhorani Rojin , Mammadova Jamila , Wang Zhigao
Published: November 2023

Mixed lineage kinase-like protein (MLKL) forms amyloid-like polymers to promote necroptosis; however, the mechanism through which these polymers trigger cell death is not clear. We have determined that activated MLKL translocates to the lysosomal membrane during necroptosis induction. The subsequent polymerization of MLKL induces lysosome clustering and fusion and eventual lysosomal membrane permeabilization (LMP). This LMP leads to the rapid release of lysosomal contents into the cytosol, resulting in a massive surge in cathepsin levels, with Cathepsin B (CTSB) as a significant contributor to the ensuing cell death as it cleaves many proteins essential for cell survival. Importantly, chemical inhibition or knockdown of CTSB protects cells from necroptosis. Furthermore, induced polymerization of the MLKL N-terminal domain (NTD) also triggers LMP, leading to CTSB release and subsequent cell death. These findings clearly establish the critical role of MLKL polymerization induced lysosomal membrane permeabilization (MPI-LMP) in the process of necroptosis.
Biofilm microenvironment triggered self-enhancing photodynamic immunomodulatory microneedle for diabetic wound therapy
Authors: Yang Li , Zhang Dan , Li Wenjing , Lin Hongbing , Ding Chendi , Liu Qingyun , Wang Liangliang , Li Zimu , Mei Lin , Chen Hongzhong , Zhao Yanli , Zeng Xiaowei
Published: November 2023

The treatment of diabetic wounds faces enormous challenges due to complex wound environments, such as infected biofilms, excessive inflammation, and impaired angiogenesis. The critical role of the microenvironment in the chronic diabetic wounds has not been addressed for therapeutic development. Herein, we develop a microneedle (MN) bandage functionalized with dopamine-coated hybrid nanoparticles containing selenium and chlorin e6 (SeC@PA), which is capable of the dual-directional regulation of reactive species (RS) generation, including reactive oxygen species (ROS) and reactive nitrogen species (RNS), in response to the wound microenvironment. The SeC@PA MN bandage can disrupt barriers in wound coverings for efficient SeC@PA delivery. SeC@PA not only depletes endogenous glutathione (GSH) to enhance the anti-biofilm effect of RS, but also degrades GSH in biofilms through cascade reactions to generate more lethal RS for biofilm eradication. SeC@PA acts as an RS scavenger in wound beds with low GSH levels, exerting an anti-inflammatory effect. SeC@PA also promotes the M2-phenotype polarization of macrophages, accelerating wound healing. This self-enhanced, catabolic and dynamic therapy, activated by the wound microenvironment, provides an approach for treating chronic wounds.
Borate–pyran lean electrolyte-based Li-metal batteries with minimal Li corrosion
Authors: Kwon Hyeokjin , Kim Hongsin , Hwang Jaemin , Oh Wonsik , Roh Youngil , Shin Dongseok , Kim Hee-Tak
Published: November 2023

Engineering liquid electrolytes for lithium (Li)-metal electrodes has been used to control the morphology of deposited Li in Li-metal batteries (LMBs). However, the Li corrosion problem remains unresolved, hindering the design of lean electrolytes for practical LMBs, which require the electrolyte/capacity (E/C) ratio to be 2 g Ah−1 or lower. Here we report a borate–pyran-based electrolyte to address the chronic Li-corrosion problem. We discovered that the borate–pyran electrolyte transforms large LiF crystallites in the solid–electrolyte interphase into fine crystalline or glassy LiF, which enhances the passivity of the Li/electrolyte interface by minimizing the permeation of electrolyte molecules into the solid–electrolyte interphase. LMBs assembled with the borate–pyran electrolyte, a high-nickel layered oxide cathode (3.83 mAh cm−2) and thin lithium (20 μm) delivered a high initial full-cell-level energy density (>400 Wh kg−1) and operated for 400 cycles with 70% capacity retention at an E/C ratio of 1.92 g Ah−1, 350 cycles with 73% capacity retention at 1.24 g Ah−1 and 200 cycles with 85% retention at 0.96 g Ah−1.
Fragment contribution models for predicting skin permeability using HuskinDB
Authors: Waters Laura J. , Cooke David J. , Quah Xin Ling
Published: November 2023

Mathematical models to predict skin permeation tend to be based on animal derived experimental data as well as knowing physicochemical properties of the compound under investigation, such as molecular volume, polarity and lipophilicity. This paper presents a strikingly contrasting model to predict permeability, formed entirely from simple chemical fragment (functional group) data and a recently released, freely accessible human (i.e. non-animal) skin permeation database, known as the ‘Human Skin Database – HuskinDB’. Data from within the database allowed development of several fragment-based models, each including a calculable effect for all of the most commonly encountered functional groups present in compounds within the database. The developed models can be applied to predict human skin permeability (logKp) for any compound containing one or more of the functional groups analysed from the dataset with no need to know any other physicochemical properties, solely the type and number of each functional group within the chemical structure itself. This approach simplifies mathematical prediction of permeability for compounds with similar properties to those used in this study.
Superiority of systemic bleomycin to intradermal HOCl for the study of interstitial lung disease
Authors: Morozan Arina , Joy Sydney , Fujii Utako , Fraser Richard , Watters Kevin , Martin James G. , Colmegna Inés
Published: November 2023

Systemic sclerosis (SSc) is an autoimmune disease characterized by vasculopathy, immune dysregulation, and multi-organ fibrosis. Interstitial lung disease (ILD) is a complication of SSc and a leading cause of SSc-death. The administration of hypochlorous acid (HOCl) intradermally in the mouse (HOCl-SSc) purportedly shows several features typical of SSc. We studied the model by injecting BALB/c mice daily intradermally with HOCl for 6-weeks, an exposure reported to induce lung fibrosis. On day 42, the skinfold thickness and the dermal thickness were two and three times larger respectively in the HOCl group compared to controls. HOCl treatment did not result in histological features of pulmonary fibrosis nor significant changes in lung compliance. Automated image analysis of HOCl mice lungs stained with picrosirius red did not show increased collagen deposition. HOCl injections did not increase pulmonary mRNA expression of pro-fibrotic genes nor induced the production of serum advanced oxidation protein products and anti-topoisomerase 1 antibodies. Immune cells in bronchoalveolar lavage fluid (BALF) and whole lung digests were not increased in HOCl-treated animals. Since lung fibrosis is proposed to be triggered by oxidative stress, we injected HOCl to Nrf2−/− mice, a mouse deficient in many antioxidant proteins. Lung compliance, histology, and BALF leukocyte numbers were comparable between Nrf2−/− mice and wild-type controls. We conclude that the HOCl-SSc model does not manifest SSc-lung disease.
Phenogrouping and risk stratification of patients undergoing cardiac resynchronization therapy upgrade using topological data analysis
Authors: Schwertner Walter Richard , Tokodi Márton , Veres Boglárka , Behon Anett , Merkel Eperke Dóra , Masszi Richárd , Kuthi Luca , Szijártó Ádám , Kovács Attila , Osztheimer István , Zima Endre , Gellér László , Vámos Máté , Sághy László , Merkely Béla , Kosztin Annamária , Becker Dávid
Published: November 2023

Choosing the optimal device during cardiac resynchronization therapy (CRT) upgrade can be challenging. Therefore, we sought to provide a solution for identifying patients in whom upgrading to a CRT-defibrillator (CRT-D) is associated with better long-term survival than upgrading to a CRT-pacemaker (CRT-P). To this end, we first applied topological data analysis to create a patient similarity network using 16 clinical features of 326 patients without prior ventricular arrhythmias who underwent CRT upgrade. Then, in the generated circular network, we delineated three phenogroups exhibiting significant differences in clinical characteristics and risk of all-cause mortality. Importantly, only in the high-risk phenogroup was upgrading to a CRT-D associated with better survival than upgrading to a CRT-P (hazard ratio: 0.454 (0.228–0.907), p = 0.025). Finally, we assigned each patient to one of the three phenogroups based on their location in the network and used this labeled data to train multi-class classifiers to enable the risk stratification of new patients. During internal validation, an ensemble of 5 multi-layer perceptrons exhibited the best performance with a balanced accuracy of 0.898 (0.854–0.942) and a micro-averaged area under the receiver operating characteristic curve of 0.983 (0.980–0.986). To allow further validation, we made the proposed model publicly available (https://github.com/tokmarton/crt-upgrade-risk-stratification).
The convenient method and application for monitoring the health of traffic sign poles based on mobile phone
Authors: Xie Xiao , Chen Yumin , Li Changchun , Guo Junwei , Sarajpoor Saeed
Published: November 2023

Traffic sign poles are crucial components of the highway system, and their maintenance mainly relies on the subjective judgment of staff, which is low-efficiency and will lead to mistaken inspections. This paper proposes a convenient and effective method to monitor the health of sign poles by using a mobile phone. It is unknown whether a mobile phone can be used as a conventional acceleration sensor. Therefore, the performance of the mobile phone was initially tested to ensure its suitability for acquiring the acceleration data of the traffic sign pole. The results show that the acceleration sensor of mobile phones is high-performance and can be used as a traditional acceleration sensor under the similar sampling frequency. The mobile phone can measure the 1st, 2nd and 5th natural frequencies of the traffic sign pole. Although the 1st and 5th natural frequencies have a large error with simulation, the 2nd natural frequency is accurate and stable. The looseness of the base connection greatly impacts on the 2nd natural frequency, which can be used as a measure of the loose base connection. The 2nd natural frequency was measured for 21 times and found to conform to a normal distribution. The relationship between the 2nd natural frequency and base connection loosening was investigated, which fitted to the equation. The fitting result is good and can be used to predict the degree of sign pole base connection loosening. Therefore, the mobile phone based convenient health monitoring method for traffic sign poles is feasible.
Sensitivity analysis of counterweight double-row pile deformation to weak stratum parameters
Authors: Wang Qiongyi , Niu Yungang , Ma Fenghai , Lu Shasha
Published: November 2023

In order to investigate the sensitivity of weak soil parameters on the deformation of balanced double-row piles, a case study was conducted in a deep foundation pit project in Shenzhen City. A variety of analysis methods, including numerical simulation, field measurements, orthogonal experiments, and theoretical analysis, were employed to analyze the impact of three weak soil parameters on the deformation of balanced double-row piles. The research results showed that the deformation of the front and back rows of piles exhibited overturning deformation, gradually decreasing with depth and reaching the maximum at the pile top due to the constraint effect of the balance platform. The numerical simulation results of horizontal displacements for the front and rear piles were in good agreement with the field measurements, confirming the accuracy and reasonableness of the numerical analysis model and parameter selection. Through a series of orthogonal numerical simulation experiments, it was determined that the cohesive strength (C) of soft layers, such as rockfill and silt, is a key factor, the internal friction angle (φ) is an important influencing factor, and the elastic modulus (E) is a general influencing factor. Theoretical analysis was employed to establish the relationship curve between each parameter and the maximum pile deformation, as well as the sensitivity factors, further verifying the impact of these weak soil parameters. The research findings presented in this paper can provide valuable guidance for geotechnical engineers when selecting geological parameters for similar deep excavation projects.
Efficacy of combined tumor irradiation and KCa3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
Authors: Stransky Nicolai , Ganser Katrin , Quintanilla-Martinez Leticia , Gonzalez-Menendez Irene , Naumann Ulrike , Eckert Franziska , Koch Pierre , Huber Stephan M. , Ruth Peter
Published: November 2023

The intermediate-conductance calcium-activated potassium channel KCa3.1 has been proposed to be a new potential target for glioblastoma treatment. This study analyzed the effect of combined irradiation and KCa3.1-targeting with TRAM-34 in the syngeneic, immune-competent orthotopic SMA-560/VM/Dk glioma mouse model. Whereas neither irradiation nor TRAM-34 treatment alone meaningfully prolonged the survival of the animals, the combination significantly prolonged the survival of the mice. We found an irradiation-induced hyperinvasion of glioma cells into the brain, which was inhibited by concomitant TRAM-34 treatment. Interestingly, TRAM-34 did neither radiosensitize nor impair SMA-560’s intrinsic migratory capacities in vitro. Exploratory findings hint at increased TGF-β1 signaling after irradiation. On top, we found a marginal upregulation of MMP9 mRNA, which was inhibited by TRAM-34. Last, infiltration of CD3+, CD8+ or FoxP3+ T cells was not impacted by either irradiation or KCa3.1 targeting and we found no evidence of adverse events of the combined treatment. We conclude that concomitant irradiation and TRAM-34 treatment is efficacious in this preclinical glioma model.
Cumulative effect of impaired fasting glucose on the risk of dementia in middle-aged and elderly people: a nationwide cohort study
Authors: Yu Jin , Lee Kyu-Na , Kim Hun-Sung , Han Kyungdo , Lee Seung-Hwan
Published: November 2023

The relationship between prediabetes and dementia remains controversial. We aimed to examine the association between cumulative exposure to impaired fasting glucose (IFG) and the risk of dementia in the general population. 1,463,066 middle-aged and elderly subjects who had had health examinations for four consecutive years were identified from a Korean nationwide population-based cohort database. IFG was defined as fasting blood glucose 100–125 mg/dL, and the risk of dementia—according to the number of IFG exposure (range 0–4)—was analyzed using the multivariable Cox proportional-hazards model. During the median 6.4 years of follow-up, 7614 cases of all-cause dementia, 5603 cases of Alzheimer’s disease, and 1257 cases of vascular dementia occurred. There was a significant trend towards a higher risk of all-cause dementia (P for trend = 0.014) and Alzheimer’s disease ( Pfor trend = 0.005) according to the cumulative exposure to IFG, but with a modest (approximately 7–14%) increase in the hazards. A significant stepwise increase in the risk of all-cause dementia and Alzheimer’s disease was seen in non-obese subjects, whereas no significant association was observed in obese subjects. This study supports the association between prediabetes and incident dementia and emphasizes that even mild hyperglycemia should not be overlooked.
Adaptor proteins mediate CXCR4 and PI4KA crosstalk in prostate cancer cells and the significance of PI4KA in bone tumor growth
Authors: Govindarajan Barani , Sbrissa Diego , Pressprich Mark , Kim Seongho , Rishi Arun K. , Vaishampayan Ulka , Cher Michael L. , Chinni Sreenivasa R.
Published: November 2023

The chemokine receptor, CXCR4 signaling regulates cell growth, invasion, and metastasis to the bone-marrow niche in prostate cancer (PCa). Previously, we established that CXCR4 interacts with phosphatidylinositol 4-kinase IIIα (PI4KIIIα encoded by PI4KA) through its adaptor proteins and PI4KA overexpressed in the PCa metastasis. To further characterize how the CXCR4–PI4KIIIα axis promotes PCa metastasis, here we identify CXCR4 binds to PI4KIIIα adaptor proteins TTC7 and this interaction induce plasma membrane PI4P production in prostate cancer cells. Inhibiting PI4KIIIα or TTC7 reduces plasma membrane PI4P production, cellular invasion, and bone tumor growth. Using metastatic biopsy sequencing, we found PI4KA expression in tumors correlated with overall survival and contributes to immunosuppressive bone tumor microenvironment through preferentially enriching non-activated and immunosuppressive macrophage populations. Altogether we have characterized the chemokine signaling axis through CXCR4–PI4KIIIα interaction contributing to the growth of prostate cancer bone metastasis.
Physical activity of mice on dietary sulfur amino acid restriction is influenced by age of diet initiation and biological sex
Authors: Cooke Diana , Ables Gene P.
Published: November 2023

Abstract Please use the attached pdf file for corrections.

Sulfur amino acid restriction (SAAR)—the reduction of methionine and cysteine concentrations either in the diet or by genetic manipulation—promotes health span and extends lifespan, but its effects on physical activity remain unclear. We investigated whether age of diet initiation and biological sex could influence physical activity in mice fed either a control diet (CF, 0.86% methionine w/w) or SAAR (0.12% methionine w/w). Quadriceps femoris muscle mass is smaller in SAAR than in CF mice. Young mice fed a chronic SAAR diet at 8 weeks of age exhibited improved wire hang and running wheel activities compared to young CF mice, while aged mice showed comparable results. The effects of chronic SAAR on physical activity was mildly influenced by sex as observed in middle-aged male SAAR mice who showed minor improvements than CF males while middle-aged females displayed no discernible effects. Muscle mass is minimally affected by changes in markers of protein synthesis, autophagy and atrophy. Improvements to physical activity in young SAAR mice could be partially attributed to increased skeletal muscle mitochondrial activity. Furthermore, SAAR in C2C12 myotubes increased citrate synthase protein expression and enhanced succinyl dehydrogenase enzyme activity compared to CF myotubes. Overall, our data reveal that SAAR can improve mouse physical activity without compromising muscle proteostasis. This is partially due to enhanced mitochondrial activity, but the effects are influenced by age of diet initiation and sex.
Insight into age-related changes of the human facial skeleton based on medieval European osteological collection
Authors: Walczak Anna , Krenz-Niedbała Marta , Łukasik Sylwia
Published: November 2023

Aging changes in the facial skeleton are concentrated mostly in orbits, maxilla and mandible. The aim of this study was to analyze metric traits of the adult viscerocranium in a medieval sample from Cedynia (Poland, Central Europe) and confront the results with literature data for modern populations. It was assumed that diet-related greater biomechanical forces generated during mastication in medieval versus modern times led to slower rate of bone resorption with age. 3D models of the facial skeleton are created for 230 individuals, categorized into young, middle and old adults, and a subgroup of edentulous middle adults is distinguished. Orbits, piriform aperture, maxilla and mandible are measured using Geomagic Studio 12 and analyzed among age categories as well as dentate and edentulous subgroups. The values of the orbital and piriform aperture measurements tend to increase with age and reached statistical significance in males (right orbit height, left orbit width, piriform aperture surface area). In females, maxillary height significantly decrease at right first premolar and first molar, together with height of the right mandibular ramus. In edentulous individuals of both sexes the orbits are wider, and maxillary and mandibular heights are lower than in dentate individuals. This study reveals similar character and direction of the aging process of the facial skeleton in medieval and modern adults, however slower rate of resorptive changes is found in the former sample, which suggests, that diet-related biomechanical forces can influence intensification of the aging processes in the facial skeleton.
Study on the factors of hydrogen sulfide production from lignite bacterial sulfate reduction based on response surface method
Authors: Deng Qigen , Li Shuai , Yao Mengmeng , Liu Chaosi , Zhang Zhecheng , Xiang Sisi
Published: November 2023

Bacterial sulfate reduction (BSR) is one of the key factors leading to the anomalous accumulation of hydrogen sulphide in coal mines. Environmental factors such as temperature and pH play a crucial role in the metabolism and degradation of coal by sulfate-reducing bacteria (SRB). In this study, coal samples were selected from Shengli Coal Mine, and SRB strains were isolated and purified from mine water using a dilution spread-plate anaerobic cultivation method. Based on single-factor experiments and response surface methodology (RSM), the impact of temperature, pH, oxidation–reduction potential (ORP), chemical oxygen demand to sulfate ratio (COD/SO42−) on the generation of hydrogen sulphide during brown coal BSR was analyzed. The results showed that the anaerobic degradation of coal by SRB was inhibited by either too high or too low a temperature to produce hydrogen sulfide, and the greatest production of hydrogen sulfide occurred at a temperature of about 30 °C; The greatest production of hydrogen sulfide occurred at an initial ambient pH of 7.5; COD/SO42− ratio of around 2.0 is most conducive to hydrogen sulphide generation; the lower ORP value is more favorable for hydrogen sulfide generation. The optimal conditions obtained by RSM were: temperature of 30.37 °C, pH of 7.64 and COD/SO42− of 1.96. Under these conditions, the hydrogen sulfide concentration was 56.79 mg/L, the pH value was 8.40, the ORP value was −274 mV, and the SO42− utilization rate was 58.04%. The RSM results showed that temperature, ambient pH and COD/SO42− had a significant effect on hydrogen sulfide production, and the degree of effect was: ambient pH > temperature > COD/SO42−.
Morphotype induced changes in the life history and population dynamics of an hippolytid shrimp
Authors: Anastasiadou Chryssa , Liasko Roman , Leonardos Ioannis
Published: November 2023

One of the most exceptional, loud paradigm of shape polymorphisms constitutes the “rostral loss” condition in hippolytid shrimps. The intertidal shrimp Hippolyte sapphica includes two conspecific morphotypes, one of which demonstrates a neotenic rostrum (morph-B). Morphs’ rostral elongation is controlled by a single genetic locus, with long rostra (morph-A) representing the recessive state and short, larval-like ones the completely dominant state. Geometrics morphometry on the species morphotypes revealed also the homozygous/heterozygous state of the gene site along with some induced body’ adaptations, which compete the micro-evolutionary disadvantage of the “rostral loss”. We found recently that females’ viability and maternal energy investment selectively favors morph-A. The present contribution detects and discuss comparatively demographic and reproductive traits in species mixed (both morphs) and unmixed populations. Our results show that this sharp dimorphic rostral condition is a sex-related marker and that the species is gonochoric. Presence of morph-B results to (a) lower egg production (b) higher seasonally males’ percentage (c) morph-A females’ earlier maturation and (d) higher fecundity in morph-A mixed populations. It seems that the “rostral loss” state induces complex adaptations between the two morphotypes through sex ratios equilibria, morphotypes’ growth rates, and morphs’ fecundity differentiate inputs throughout the seasons.
Multi-scale measurement of stiffness in the developing ferret brain
Authors: Walter Christopher , Balouchzadeh Ramin , Garcia Kara E. , Kroenke Christopher D. , Pathak Amit , Bayly Philip V.
Published: November 2023

Cortical folding is an important process during brain development, and aberrant folding is linked to disorders such as autism and schizophrenia. Changes in cell numbers, size, and morphology have been proposed to exert forces that control the folding process, but these changes may also influence the mechanical properties of developing brain tissue. Currently, the changes in tissue stiffness during brain folding are unknown. Here, we report stiffness in the developing ferret brain across multiple length scales, emphasizing changes in folding cortical tissue. Using rheometry to measure the bulk properties of brain tissue, we found that overall brain stiffness increases with age over the period of cortical folding. Using atomic force microscopy to target the cortical plate, we found that the occipital cortex increases in stiffness as well as stiffness heterogeneity over the course of development and folding. These findings can help to elucidate the mechanics of the cortical folding process by clarifying the concurrent evolution of tissue properties.
Nucleotide excision repair deficiency is a targetable therapeutic vulnerability in clear cell renal cell carcinoma
Authors: Prosz Aurel , Duan Haohui , Tisza Viktoria , Sahgal Pranshu , Topka Sabine , Klus Gregory T. , Börcsök Judit , Sztupinszki Zsofia , Hanlon Timothy , Diossy Miklos , Vizkeleti Laura , Stormoen Dag Rune , Csabai Istvan , Pappot Helle , Vijai Joseph , Offit Kenneth , Ried Thomas , Sethi Nilay , Mouw Kent W. , Spisak Sandor , Pathania Shailja , Szallasi Zoltan
Published: November 2023

Due to a demonstrated lack of DNA repair deficiencies, clear cell renal cell carcinoma (ccRCC) has not benefitted from targeted synthetic lethality-based therapies. We investigated whether nucleotide excision repair (NER) deficiency is present in an identifiable subset of ccRCC cases that would render those tumors sensitive to therapy targeting this specific DNA repair pathway aberration. We used functional assays that detect UV-induced 6–4 pyrimidine-pyrimidone photoproducts to quantify NER deficiency in ccRCC cell lines. We also measured sensitivity to irofulven, an experimental cancer therapeutic agent that specifically targets cells with inactivated transcription-coupled nucleotide excision repair (TC-NER). In order to detect NER deficiency in clinical biopsies, we assessed whole exome sequencing data for the presence of an NER deficiency associated mutational signature previously identified in ERCC2 mutant bladder cancer. Functional assays showed NER deficiency in ccRCC cells. Some cell lines showed irofulven sensitivity at a concentration that is well tolerated by patients. Prostaglandin reductase 1 (PTGR1), which activates irofulven, was also associated with this sensitivity. Next generation sequencing data of the cell lines showed NER deficiency-associated mutational signatures. A significant subset of ccRCC patients had the same signature and high PTGR1 expression. ccRCC cell line-based analysis showed that NER deficiency is likely present in this cancer type. Approximately 10% of ccRCC patients in the TCGA cohort showed mutational signatures consistent with ERCC2 inactivation associated NER deficiency and also substantial levels of PTGR1 expression. These patients may be responsive to irofulven, a previously abandoned anticancer agent that has minimal activity in NER-proficient cells.
YFDM: YOLO for detecting Morse code
Authors: Wei Zhenhua , Li Zijun , Han Siming
Published: November 2023

With the increasing complexity of the shortwave communication environment, the efficiency and accuracy of the manual detection of Morse code no longer meet actual needs. Therefore, this paper proposes a Morse code detection algorithm called YFDM. For the time–frequency image of the received signal, a combination module of deformable convolution and C3 is used to enhance the backbone network’s attention to the abstract semantics and location information of Morse code. GSConv and VOV-GSCSP modules are used to build a lightweight neck network. Finally, the confidence propagation cluster (CP-Cluster) algorithm is used to filter the detection frame. In an ablation experiment, the parameters and giga floating-point operations per second (GFLOPs) of YFDM were 5.961 M and 9.74 G, respectively, 15.11% and 38.9% less than those of YOLOv5. Moreover, when WIoUv1 was used as the loss function of the bounding box, the AP0.5:0.95 and frames per second (FPS) values of the algorithm reached the highest values, 0.68 and 72.4. The experimental results indicate that the algorithm can effectively reduce the weight of the model while ensuring the detection accuracy and inference speed.
Efficacy and safety of second-line cabozantinib after immuno-oncology combination therapy for advanced renal cell carcinoma: Japanese multicenter retrospective study
Authors: Sazuka Tomokazu , Matsushita Yuto , Sato Hiroaki , Osawa Takahiro , Hinata Nobuyuki , Hatakeyama Shingo , Numakura Kazuyuki , Ueda Kosuke , Kimura Takahiro , Takahashi Masayuki , Tanaka Hajime , Kawasaki Yoshihide , Kurahashi Toshifumi , Kato Takuma , Fujita Kazutoshi , Miyake Makito , Kojima Takahiro , Kitamura Hiroshi , Miyake Hideaki , Ichikawa Tomohiko
Published: November 2023

Immuno-oncology (IO) combination therapy is utilized as a first-line systemic treatment for advanced renal cell carcinoma. However, evidence supporting the use of cabozantinib after IO combination therapy is lacking. We retrospectively analyzed patients who received second-line cabozantinib after IO combination therapy using the Japanese Urological Oncology Group (JUOG) database. In total, 254 patients were enrolled in the JUOG global study, and 118 patients who received second-line cabozantinib comprised the study cohort. The objective response rate, disease control rate, second-line cabozantinib progression-free survival (PFS), and overall survival from second-line for overall were 32%, 75%, 10.5 months, and not reached, respectively, for first-line IO-IO therapy were 37%, 77%, 11.1 months, and not reached, respectively, versus 24%, 71%, 8.3 months, and not reached, respectively, for first-line IO-tyrosine kinase inhibitor therapy. In univariate and multivariate analyses, discontinuation of first-line treatment because of progressive disease and liver metastasis were independent risk factors for PFS. All-grade adverse events occurred in 72% of patients, and grade 3 or higher adverse events occurred in 28% of patients. Second line-cabozantinib after first-line IO combination therapy for advanced renal cell carcinoma was expected to be effective after either IO-IO or IO-TKI treatment and feasible in real-world practice.
Parapapillary choroidal microvasculature dropout in eyes with primary open-angle glaucoma
Authors: Igarashi Ryoko , Ochiai Shun , Akagi Tadamichi , Miyamoto Daiki , Sakaue Yuta , Iikawa Ryu , Fukuchi Takeo
Published: November 2023

The purpose of this study was to evaluate how various parameters are related to microvasculature dropout (MvD) area measured using optical coherence tomography angiography (OCTA). We measured the area of MvD in 55 patients with primary open-angle glaucoma (POAG). Using OCTA, MvD area and peripapillary choroidal atrophy (PPA) area were assessed in a 4.5 mm × 4.5 mm region. The following were examined: circumpapillary nerve fiber layer (cpRNFL) thickness, optic disc area, optic disc cupping area, optic disc rim area, Humphrey Field Analyzer (HFA) 24/10–2 mean deviation (MD), and pattern standard deviation (PSD). The relationship between MvD area and each parameter was evaluated using Spearman’s rank correlation coefficient analysis. Mean MvD area and PPA area were 0.18 ± 0.17 mm2 and 1.13 ± 0.72 mm2, respectively. MvD area was significantly correlated with optic disc rim area (p = 0.0017), cpRNFL (p = 0.0027), HFA 24/10–2 MD, and PSD (p < 0.001). In eyes with POAG, MvD area indicates the severity of glaucoma, which might be associated with structural changes in the peripapillary vasculature around the optic disc.
Exploring complexity of class-A Beta-lactamase family using physiochemical-based multiplex networks
Authors: Bhadola Pradeep , Deo Nivedita
Published: November 2023

The Beta-lactamase protein family is vital in countering Beta-lactam antibiotics, a widely used antimicrobial. To enhance our understanding of this family, we adopted a novel approach employing a multiplex network representation of its multiple sequence alignment. Each network layer, derived from the physiochemical properties of amino acids, unveils distinct insights into the intricate interactions among nodes, thereby enabling the identification of key motifs. Nodes with identical property signs tend to aggregate, providing evidence of the presence of consequential functional and evolutionary constraints shaping the Beta-lactamase family. We further investigate the distribution of evolutionary links across various layers. We observe that polarity manifests the highest number of unique links at lower thresholds, followed by hydrophobicity and polarizability, wherein hydrophobicity exerts dominance at higher thresholds. Further, the combinations of polarizability and volume, exhibit multiple simultaneous connections at all thresholds. The combination of hydrophobicity, polarizability, and volume uncovers shared links exclusive to these layers, implying substantial evolutionary impacts that may have functional or structural implications. By assessing the multi-degree of nodes, we unveil the hierarchical influence of properties at each position, identifying crucial properties responsible for the protein’s functionality and providing valuable insights into potential targets for modulating enzymatic activity.
Discriminating mixed qubit states with collective measurements
Authors: Conlon Lorcán O. , Eilenberger Falk , Lam Ping Koy , Assad Syed M.
Published: November 2023

It is a central fact in quantum mechanics that non-orthogonal states cannot be distinguished perfectly. In general, the optimal measurement for distinguishing such states is a collective measurement. However, to the best our knowledge, collective measurements have not been used to enhance quantum state discrimination to date. One of the main reasons for this is the fact that, in the usual state discrimination setting with equal prior probabilities, at least three copies of a quantum state are required to be measured collectively to outperform separable measurements. This is very challenging experimentally. In this work, by considering unequal prior probabilities, we propose and experimentally demonstrate a protocol for distinguishing two copies of single qubit states using collective measurements which achieves a lower probability of error than can be achieved by any non-entangling measurement. Additionally, we implemented collective measurements on three and four copies of the unknown state and found they performed poorly.