bluuebunny/crossref_metadata_2025 huggingface dataset:
A Validated LC–MS/MS Assay for the Simultaneous Quantification of the FDA-Approved Anticancer Mixture (Encorafenib and Binimetinib): Metabolic Stability Estimation
Authors: Attwa Mohamed W. , Darwish Hany W. , Al-Shakliah Nasser S. , Kadi Adnan A.
Published: May 2021

The concurrent use of oral encorafenib (Braftovi, ENF) and binimetinib (Mektovi, BNB) is a combination anticancer therapy approved by the United States Food and Drug Administration (USFDA) for patients with BRAFV600E/V600K mutations suffering from metastatic or unresectable melanoma. Metabolism is considered one of the main pathways of drug elimination from the body (responsible for elimination of about 75% of known drugs), it is important to understand and study drug metabolic stability. Metabolically unstable compounds are not good as they required repetitive dosages during therapy, while very stable drugs may result in increasing the risk of adverse drug reactions. Metabolic stability of compounds could be examined using in vitro or in silico experiments. First, in silico metabolic vulnerability for ENF and BNB was investigated using the StarDrop WhichP450 module to confirm the lability of the drugs under study to liver metabolism. Second, we established an LC–MS/MS method for the simultaneous quantification of ENF and BNB applied to metabolic stability assessment. Third, in silico toxicity assessment of ENF and BNB was performed using the StarDrop DEREK module. Chromatographic separation of ENF, BNB, and avitinib (an internal standard) was achieved using an isocratic mobile phase on a Hypersil BDS C18 column. The linear range for ENF and BNB in the human liver microsome (HLM) matrix was 5–500 ng/mL (R2 ≥ 0.999). The metabolic stabilities were calculated using intrinsic clearance and in vitro half-life. Furthermore, ENF and BNB did not significantly influence each other’s metabolic stability or metabolic disposition when used concurrently. These results indicate that ENF and BNB will slowly bioaccumulate after multiple doses.
Systematic Study of Quaternary Ammonium Cations for Bromine Sequestering Application in High Energy Density Electrolytes for Hydrogen Bromine Redox Flow Batteries
Authors: Küttinger Michael , Loichet Torres Paulette A. , Meyer Emeline , Fischer Peter , Tübke Jens
Published: May 2021

Bromine complexing agents (BCAs) are used to reduce the vapor pressure of bromine in the aqueous electrolytes of bromine flow batteries. BCAs bind hazardous, volatile bromine by forming a second, heavy liquid fused salt. The properties of BCAs in a strongly acidic bromine electrolyte are largely unexplored. A total of 38 different quaternary ammonium halides are investigated ex situ regarding their properties and applicability in bromine electrolytes as BCAs. The focus is on the development of safe and performant HBr/Br2/H2O electrolytes with a theoretical capacity of 180 Ah L−1 for hydrogen bromine redox flow batteries (H2/Br2-RFB). Stable liquid fused salts, moderate bromine complexation, large conductivities and large redox potentials in the aqueous phase of the electrolytes are investigated in order to determine the most applicable BCA for this kind of electrolyte. A detailed study on the properties of BCA cations in these parameters is provided for the first time, as well as for electrolyte mixtures at different states of charge of the electrolyte. 1-ethylpyridin-1-ium bromide [C2Py]Br is selected from 38 BCAs based on its properties as a BCA that should be focused on for application in electrolytes for H2/Br2-RFB in the future.
Polysaccharide-Based Drug Delivery Systems for the Treatment of Periodontitis
Authors: Baranov Nicolae , Popa Marcel , Atanase Leonard Ionut , Ichim Daniela Luminita
Published: May 2021

Periodontal diseases are worldwide health problems that negatively affect the lifestyle of many people. The long-term effect of the classical treatments, including the mechanical removal of bacterial plaque, is not effective enough, causing the scientific world to find other alternatives. Polymer–drug systems, which have different forms of presentation, chosen depending on the nature of the disease, the mode of administration, the type of polymer used, etc., have become very promising. Hydrogels, for example (in the form of films, micro-/nanoparticles, implants, inserts, etc.), contain the drug included, encapsulated, or adsorbed on the surface. Biologically active compounds can also be associated directly with the polymer chains by covalent or ionic binding (polymer–drug conjugates). Not just any polymer can be used as a support for drug combination due to the constraints imposed by the fact that the system works inside the body. Biopolymers, especially polysaccharides and their derivatives and to a lesser extent proteins, are preferred for this purpose. This paper aims to review in detail the biopolymer–drug systems that have emerged in the last decade as alternatives to the classical treatment of periodontal disease.
The Study of Derivatization Prior MALDI MSI Analysis—Charge Tagging Based on the Cholesterol and Betaine Aldehyde
Authors: Mielczarek Przemyslaw , Slowik Tymoteusz , Kotlinska Jolanta Helena , Suder Piotr , Bodzon-Kulakowska Anna
Published: May 2021

Mass spectrometry imaging is a powerful tool for analyzing the different kinds of molecules in tissue sections, but some substances cannot be measured easily, due to their physicochemical properties. In such cases, chemical derivatization could be applied to introduce the charge into the molecule and facilitate its detection. Here, we study cholesterol derivatization with betaine aldehyde from tissue slices and evaluate how different sample preparation methods influence the signal from the derivatization product. In this study, we have tested different solutions for betaine aldehyde, different approaches to betaine aldehyde deposition (number of layers, deposition nozzle height), and different MALDI matrices for its analysis. As a result, we proved that the proposed approach could be used for the analysis of cholesterol in different tissues.
Determination of Polycyclic Aromatic Hydrocarbons and Their Methylated Derivatives in Sewage Sludge from Northeastern China: Occurrence, Profiles and Toxicity Evaluation
Authors: Mohammed Rashid , Zhang Zi-Feng , Kan Ze , Jiang Chao , Liu Li-Yan , Ma Wan-Li , Song Wei-Wei , Nikolaev Anatoly , Li Yi-Fan
Published: May 2021

This paper assesses the occurrence, distribution, source, and toxicity of polycyclic aromatic hydrocarbons (PAHs), and their methylated form (Me-PAHs) in sewage sludge from 10 WWTPs in Northeastern China was noted. The concentrations of ∑PAHs, ∑Me-PAHs ranged from 567 to 5040 and 48.1 to 479 ng.g−1dw, which is greater than the safety limit for sludge in agriculture in China. High and low molecular weight 4 and 2-ring PAHs and Me-PAHs in sludge were prevalent. The flux of sludge PAHs and Me-PAHs released from ten WWTPs, in Heilongjiang province, was calculated to be over 100 kg/year. Principal component analysis (PCA), diagnostic ratios and positive matrix factorization (PMF) determined a similar mixed pyrogenic and petrogenic source of sewage sludge. The average values of Benzo[a]pyrene was below the safe value of 600 ng.g−1 dependent on an incremental lifetime cancer risk ILCR of 10−6. Sludge is an important source for the transfer of pollutants into the environment, such as PAHs and Me-PAHs. Consequently, greater consideration should be given to its widespread occurrence.
Haptic Actuation Plate for Multi-Layered In-Vehicle Control Panel
Authors: Coe Patrick , Evreinov Grigori , Sinivaara Hasse , Hippula Arto , Raisamo Roope
Published: May 2021

High-fidelity localized feedback has the potential of providing new and unique levels of interaction with a given device. Achieving this in a cost-effective reproducible manner has been a challenge in modern technology. Past experiments have shown that by using the principles of constructive wave interference introduced by time offsets it is possible to achieve a position of increased vibration displacement at any given location. As new interface form factors increasingly incorporate curved surfaces, we now show that these same techniques can successfully be applied and mechanically coupled with a universal actuation plate.
Teleoperation of Highly Automated Vehicles in Public Transport: User-Centered Design of a Human-Machine Interface for Remote-Operation and Its Expert Usability Evaluation
Authors: Kettwich Carmen , Schrank Andreas , Oehl Michael
Published: May 2021

Paving the way to future mobility, teleoperation of vehicles promises a reachable solution to effectively use the benefits of automated driving as long as fully automated vehicles (SAE 5) are not entirely feasible. Safety and reliability are assured by a human operator who remotely observes the vehicle and takes over control in cases of disturbances that exceed the vehicle automation’s skills. In order to integrate the vehicle’s automation and human remote-operation, we developed a novel user-centered human-machine interface (HMI) for teleoperation. It is tailored to the remote-operation of a highly automated shuttle (SAE 4) by a public transport control center and based on a systematic analysis of scenarios, of which detailed requirements were derived. Subsequently, a paper-pencil prototype was generated and refined until a click-dummy emerged. This click-dummy was evaluated by twelve control center professionals. The experts were presented the prototype in regular mode and were then asked to solve three scenarios with disturbances in the system. Using structured interview and questionnaire methodology, the prototype was evaluated regarding its usability, situation awareness, acceptance, and perceived workload. Results support our HMI design for teleoperation of a highly automated shuttle, especially regarding usability, acceptance, and workload. Participant ratings and comments indicated particularly high satisfaction with the interaction design to resolve disturbances and the presentation of camera images. Participants’ feedbacks provide valuable information for a refined HMI design as well as for further research.
The Effect of Doping on the Digital Etching of Silicon-Selective Silicon–Germanium Using Nitric Acids
Authors: Li Yangyang , Zhu Huilong , Kong Zhenzhen , Zhang Yongkui , Ai Xuezheng , Wang Guilei , Wang Qi , Liu Ziyi , Lu Shunshun , Xie Lu , Huang Weixing , Liu Yongbo , Li Chen , Li Junjie , Lin Hongxiao , Su Jiale , Zeng Chuanbin , Radamson Henry H.
Published: May 2021

Gate-all-around (GAA) field-effect transistors have been proposed as one of the most important developments for CMOS logic devices at the 3 nm technology node and beyond. Isotropic etching of silicon–germanium (SiGe) for the definition of nano-scale channels in vertical GAA CMOS and tunneling FETs has attracted more and more attention. In this work, the effect of doping on the digital etching of Si-selective SiGe with alternative nitric acids (HNO3) and buffered oxide etching (BOE) was investigated in detail. It was found that the HNO3 digital etching of SiGe was selective to n+-Si, p+-Si, and intrinsic Si. Extensive studies were performed. It turned out that the selectivity of SiGe/Si was dependent on the doped types of silicon and the HNO3 concentration. As a result, at 31.5% HNO3 concentration, the relative etched amount per cycle (REPC) and the etching selectivity of Si0.72Ge0.28 for n+-Si was identical to that for p+-Si. This is particularly important for applications of vertical GAA CMOS and tunneling FETs, which have to expose both the n+ and p+ sources/drains at the same time. In addition, the values of the REPC and selectivity were obtained. A controllable etching rate and atomically smooth surface could be achieved, which enhanced carrier mobility.
Feasibility Synthesis and Characterization of Gadolinia Doped Ceria Coatings Obtained by Cathodic Arc Evaporation
Authors: Briois Pascal , Aubry Eric , Ringuedé Armelle , Cassir Michel , Billard Alain
Published: May 2021

Gadolinia doped ceria coatings were elaborated by cathodic arc evaporation from a metallic Ce–Gd (90–10 at.%) target inserted into a conventional multiarc Ti evaporation target in the presence of a reactive argon–oxygen gas mixture. The structural and chemical features of these films were determined by x-ray diffraction and scanning electron microscopy. Their electrical properties were characterized using impedance spectroscopy measurements. It was shown that the as-deposited coatings crystallize in the fluorite type fcc structure of ceria and that their composition is the same as that of the target. The morphology of the coatings is influenced by the evaporation parameter (stress and droplet). The electrical measurements showed two contributions in Nyquist representation and the activation energy was slightly higher than that given in the literature data for the bulk material.
Ab Initio Theory of Photoemission from Graphene
Authors: Krasovskii Eugene
Published: May 2021

Angle-resolved photoemission from monolayer and bilayer graphene is studied based on an ab initio one-step theory. The outgoing photoelectron is represented by the time-reversed low energy electron diffraction (LEED) state ΦLEED*, which is calculated using a scattering theory formulated in terms of augmented plane waves. A strong enhancement of the emission intensity is found to occur around the scattering resonances. The effect of the photoelectron scattering by the underlying substrate on the polarization dependence of the photocurrent is discussed. The constant initial state spectra I(k||,ℏω) are compared to electron transmission spectra T(E) of graphene, and the spatial structure of the outgoing waves is analyzed. It turns out that the emission intensity variations do not correlate with the structure of the T(E) spectra and are caused by rather subtle interference effects. Earlier experimental observations of the photon energy and polarization dependence of the emission intensity I(k||,ℏω) are well reproduced within the dipole approximation, and the Kohn–Sham eigenstates are found to provide a quite reasonable description of the photoemission final states.
Atomistic Simulations of Defect Production in Monolayer and Bulk Hexagonal Boron Nitride under Low- and High-Fluence Ion Irradiation
Authors: Ghaderzadeh Sadegh , Kretschmer Silvan , Ghorbani-Asl Mahdi , Hlawacek Gregor , Krasheninnikov Arkady V.
Published: May 2021

Controlled production of defects in hexagonal boron nitride (h-BN) through ion irradiation has recently been demonstrated to be an effective tool for adding new functionalities to this material, such as single-photon generation, and for developing optical quantum applications. Using analytical potential molecular dynamics, we study the mechanisms of vacancy creation in single- and multi-layer h-BN under low- and high-fluence ion irradiation. Our results quantify the densities of defects produced by noble gas ions in a wide range of ion energies and elucidate the types and distribution of defects in the target. The simulation data can directly be used to guide the experiment aimed at the creation of defects of particular types in h-BN targets for single-photon emission, spin-selective optical transitions and other applications by using beams of energetic ions.
Defect Study and Modelling of SnX3-Based Perovskite Solar Cells with SCAPS-1D
Authors: Samiul Islam Md. , Sobayel K. , Al-Kahtani Ammar , Islam M. A. , Muhammad Ghulam , Amin N. , Shahiduzzaman Md. , Akhtaruzzaman Md.
Published: May 2021

Recent achievements, based on lead (Pb) halide perovskites, have prompted comprehensive research on low-cost photovoltaics, in order to avoid the major challenges that arise in this respect: Stability and toxicity. In this study, device modelling of lead (Pb)-free perovskite solar cells has been carried out considering methyl ammonium tin bromide (CH3NH3SnBr3) as perovskite absorber layer. The perovskite structure has been justified theoretically by Goldschmidt tolerance factor and the octahedral factor. Numerical modelling tools were used to investigate the effects of amphoteric defect and interface defect states on the photovoltaic parameters of CH3NH3SnBr3-based perovskite solar cell. The study identifies the density of defect tolerance in the absorber layer, and that both the interfaces are 1015 cm−3, and 1014 cm−3, respectively. Furthermore, the simulation evaluates the influences of metal work function, uniform donor density in the electron transport layer and the impact of series resistance on the photovoltaic parameters of proposed n-TiO2/i-CH3NH3SnBr3/p-NiO solar cell. Considering all the optimization parameters, CH3NH3SnBr3-based perovskite solar cell exhibits the highest efficiency of 21.66% with the Voc of 0.80 V, Jsc of 31.88 mA/cm2 and Fill Factor of 84.89%. These results divulge the development of environmentally friendly methyl ammonium tin bromide perovskite solar cell.
Materials, Electrical Performance, Mechanisms, Applications, and Manufacturing Approaches for Flexible Strain Sensors
Authors: Han Fei , Li Min , Ye Huaiyu , Zhang Guoqi
Published: May 2021

With the recent great progress made in flexible and wearable electronic materials, the upcoming next generation of skin-mountable and implantable smart devices holds extensive potential applications for the lifestyle modifying, including personalized health monitoring, human-machine interfaces, soft robots, and implantable biomedical devices. As a core member within the wearable electronics family, flexible strain sensors play an essential role in the structure design and functional optimization. To further enhance the stretchability, flexibility, sensitivity, and electricity performances of the flexible strain sensors, enormous efforts have been done covering the materials design, manufacturing approaches and various applications. Thus, this review summarizes the latest advances in flexible strain sensors over recent years from the material, application, and manufacturing strategies. Firstly, the critical parameters measuring the performances of flexible strain sensors and materials development contains different flexible substrates, new nano- and hybrid- materials are introduced. Then, the developed working mechanisms, theoretical analysis, and computational simulation are presented. Next, based on different material design, diverse applications including human motion detection and health monitoring, soft robotics and human-machine interface, implantable devices, and biomedical applications are highlighted. Finally, synthesis consideration of the massive production industry of flexible strain sensors in the future; different fabrication approaches that are fully expected are classified and discussed.
Mass Transfer Study on Improved Chemistry for Electrodeposition of Copper Indium Gallium Selenide (CIGS) Compound for Photovoltaics Applications
Authors: Saeed Mahfouz , González Peña Omar Israel
Published: May 2021

Copper indium gallium selenium (CIGS) films are attractive for photovoltaic applications due to their high optical absorption coefficient. The generation of CIGS films by electrodeposition is particularly appealing due to the relatively low capital cost and high throughput. Numerous publications address the electrodeposition of CIGS; however, very few recognize the critical significance of transport in affecting the composition and properties of the compound. This study introduces a new electrolyte composition, which is far more dilute than systems that had been previously described, which yields much improved CIGS films. The electrodeposition experiments were carried out on a rotating disk electrode, which provides quantitative control of the transport rates. Experiments with the conventional electrolyte, ten times more concentrated than the new electrolyte proposed here, yielded powdery and non-adherent deposit. By contrast, the new, low concentration electrolyte produced in the preferred potential interval of −0.64 ≤ E ≤ −0.76 V vs. NHE, a smooth and adherent uniform deposit with the desired composition across a broad range of rotation speeds. The effects of mass transport on the deposit are discussed. Sample polarization curves at different electrode rotation rates, obtained in deposition experiments from the high and the low concentration electrolytes, are critically compared. Characterization of the overall efficiency, quantum efficiency, open circuit voltage, short circuit current, dark current, band gap, and the fill factor are reported.
Mechanical and Thermal Evaluation of Aluminum Hybrid Nanocomposite Reinforced with Alumina and Graphene Oxide
Authors: Mohammed Abdul Samad , Alahmari Tawfeeq Saad , Laoui Tahar , Hakeem Abbas Saeed , Patel Faheemuddin
Published: May 2021

Aluminum matrix composites are among the most widely used metal matrix composites in several industries, such as aircraft, electronics, automobile, and aerospace, due to their high specific strength, durability, structural rigidity and high corrosion resistance. However, owing to their low hardness and wear resistance, their usage is limited in demanding applications, especially in harsh environments. In the present work, aluminum hybrid nanocomposite reinforced with alumina (Al2O3) and graphene oxide (GO) possessing enhanced mechanical and thermal properties was developed using spark plasma sintering (SPS) technique. The focus of the study was to optimize the concentration of Al2O3 and GO content in the composite to improve the mechanical and thermal properties such as hardness, compressive strength, heat flow, and thermal expansion. The nanocomposites were characterized by FESEM, EDS, XRD and Raman spectroscopy to investigate their morphology and structural properties. In the first phase, different volume percent of alumina (10%, 20%, 30%) were used as reinforcement in the aluminum matrix to obtain (Al+X% Al2O3) composite with the best mechanical/thermal properties which was found to be 10 V% of Al2O3. In the second phase, a hybrid nanocomposite was developed by reinforcing the (Al + 10 V% Al2O3) with different weight percent (0.25%, 0.5%, 1%) of GO to obtain the optimum composition with improved mechanical/thermal properties. Results revealed that the Al\10 V% Al2O3\0.25 wt.% GO hybrid nanocomposite showed the highest improvement of about 13% in hardness and 34% in compressive strength as compared to the Al\10V% Al2O3 composite. Moreover, the hybrid nanocomposite Al\10 V% Al2O3\0.25 wt.% GO also displayed the lowest thermal expansion.
Catalytic Methane Decomposition to Carbon Nanostructures and COx-Free Hydrogen: A Mini-Review
Authors: Gamal Ahmed , Eid Kamel , El-Naas Muftah H. , Kumar Dharmesh , Kumar Anand
Published: May 2021

Catalytic methane decomposition (CMD) is a highly promising approach for the rational production of relatively COx-free hydrogen and carbon nanostructures, which are both important in multidisciplinary catalytic applications, electronics, fuel cells, etc. Research on CMD has been expanding in recent years with more than 2000 studies in the last five years alone. It is therefore a daunting task to provide a timely update on recent advances in the CMD process, related catalysis, kinetics, and reaction products. This mini-review emphasizes recent studies on the CMD process investigating self-standing/supported metal-based catalysts (e.g., Fe, Ni, Co, and Cu), metal oxide supports (e.g., SiO2, Al2O3, and TiO2), and carbon-based catalysts (e.g., carbon blacks, carbon nanotubes, and activated carbons) alongside their parameters supported with various examples, schematics, and comparison tables. In addition, the review examines the effect of a catalyst’s shape and composition on CMD activity, stability, and products. It also attempts to bridge the gap between research and practical utilization of the CMD process and its future prospects.
Recent Advances in the Use of Iron–Gold Hybrid Nanoparticles for Biomedical Applications
Authors: Tarkistani Mariam Abdulaziz M. , Komalla Varsha , Kayser Veysel
Published: May 2021

Recently, there has been an increased interest in iron–gold-based hybrid nanostructures, due to their combined outstanding optical and magnetic properties resulting from the usage of two separate metals. The synthesis of these nanoparticles involves thermal decomposition and modification of their surfaces using a variety of different methods, which are discussed in this review. In addition, different forms such as core–shell, dumbbell, flower, octahedral, star, rod, and Janus-shaped hybrids are discussed, and their unique properties are highlighted. Studies on combining optical response in the near-infrared window and magnetic properties of iron–gold-based hybrid nanoparticles as multifunctional nanoprobes for drug delivery, magnetic–photothermal heating as well as contrast agents during magnetic and optical imaging and magnetically-assisted optical biosensing to detect traces of targeted analytes inside the body has been reviewed.
Fulminant Guillain–Barré Syndrome Post Hemorrhagic Stroke: Two Case Reports
Authors: Abdulmana Sameeh , Al-Zahrani Naif , Sharahely Yahya , Bashir Shahid , M. Al-Harbi Talal
Published: May 2021

Guillain–Barré syndrome (GBS) is an acute, immune-mediated inflammatory peripheral polyneuropathy characterized by ascending paralysis. Most GBS cases follow gastrointestinal or chest infections. Some patients have been reported either following or concomitant with head trauma, neurosurgical procedures, and rarely hemorrhagic stroke. The exact pathogenesis is not entirely understood. However, blood–brain barrier damage may play an essential role in triggering the autoimmune activation that leads to post-stroke GBS. Here, we present two cases of fulminant GBS following hemorrhagic stroke to remind clinicians to be aware of this rare treatable complication if a stroke patient develops unexplainable flaccid paralysis with or without respiratory distress.
The Joint Effect of Maternal Marital Status and Type of Household Cooking Fuel on Child Nutritional Status in Sub-Saharan Africa: Analysis of Cross-Sectional Surveys on Children from 31 Countries
Authors: Amadu Iddrisu , Seidu Abdul-Aziz , Duku Eric , Okyere Joshua , Hagan John Elvis , Hormenu Thomas , Ahinkorah Bright Opoku
Published: May 2021

The current study sought to investigate the joint effect of maternal marital status and type of household cooking fuel on child nutritional status in sub-Saharan Africa. Data in the children’s files of 31 sub-Saharan African countries were pooled from the Demographic and Health Surveys collected between 2010 and 2019. The outcome variables were three child anthropometrics: stunting (height-for-age z-scores); wasting (weight-for-height z-scores); and underweight (weight-for-age z-scores). The joint effect of maternal marital status and type of household cooking fuel on child nutritional status was examined using multilevel regression models. The results were presented as adjusted odds ratios (aORs) at p < 0.05. The percentages of children who were stunted, wasted and underweight in the 31 countries in sub-Saharan Africa were 31%, 8% and 17%, respectively. On the joint effect of maternal marital status and type of household cooking fuel on stunting, we found that compared to children born to married mothers who used clean household cooking fuel, children born to single mothers who use unclean household cooking fuel, children born to single women who use clean household cooking fuel, and children born to married women who used unclean household cooking were more likely to be stunted. With wasting, children born to single mothers who used unclean household cooking fuel and children born to married women who used unclean household cooking fuel were more likely to be wasted compared to children born to married mothers who used clean household cooking fuel. With underweight, we found that compared to children born to married mothers who used clean household cooking fuel, children born to single mothers who used unclean household cooking fuel, children born to single women who used clean household cooking fuel and children born to married women who used unclean household cooking were more likely to be underweight. It is imperative for the governments of the 31 sub-Saharan African countries to double their efforts to end the use of unclean household cooking fuel. This goal could be achieved by promoting clean household cooking fuel (e.g., electricity, gas, ethanol, solar, etc.) through effective health education, and promotion programmes. The attention of policymakers is drawn to the urgent need for children’s nutritional status policies and programmes (e.g., dietary supplementation, increasing dietary diversity, improving agriculture and food security) to be targeted towards at-risk sub-populations (i.e., single mothered households).
Regulation and Consumer Interest in an Antioxidant-Enriched Ham Associated with Reduced Colorectal Cancer Risks
Authors: Marette Stéphan , Guéraud Françoise , Pierre Fabrice
Published: May 2021

An economic experiment was conducted in France in 2020 to evaluate consumer attitudes toward two ham products associated with different colorectal cancer risks. We focused specifically on comparing a conventional ham and a new hypothetical antioxidant-enriched ham with a reduced risk of provoking colorectal cancer. Study participants were given descriptions of the two hams before carrying out successive rounds of willingness-to-pay (WTP) assessments. The results show that WTP was higher for the antioxidant-enriched ham than for the conventional ham. WTP estimates were also impacted by providing additional information about the reduction in colorectal cancer risk associated with the antioxidant-enriched ham. Based on the participants’ WTP, we came up with ex ante estimates for the social impacts of introducing the antioxidant-enriched ham onto the market, and we suggest that it would be socially optimal to promote the product. Competition arising from pre-existing product labelling and marketing assertions could greatly limit the market potential of antioxidant-enriched ham, which suggests that alternative approaches may be necessary, such as regulations mandating antioxidant enrichment. These results also concern all countries with high levels of meat consumption.
Fermented Deer Blood Ameliorates Intense Exercise-Induced Fatigue via Modulating Small Intestine Microbiota and Metabolites in Mice
Authors: Cui Jingwen , Shi Chao , Xia Peibin , Ning Ke , Xiang Hongyu , Xie Qiuhong
Published: May 2021

Intense and excessive exercise-induced fatigue has become an important health issue and can damage intestinal health. Deer blood, as a food byproduct with nutritional value, has been found to restore physical strength. However, little is known about the antifatigue effect of fermented deer blood (FDB) on intense exercise mice. The purpose of the present study is to investigate the antifatigue effect of FDB, and whether this effect is correlated with the altered small intestinal microbiota and metabolites in exercise mice. In this study, 5-week-old male C57BL/6J mice are given treadmill exercise with or without FDB supplementation (30 and 150 mg/kg/d) for 3 weeks. FDB significantly reduces metabolic byproduct accumulation, liver and intestinal damage, and enhances glycogen storage and antioxidant capacity in intense exercise mice. Moreover, FDB restructures the small intestinal microbiota by increasing the abundance of probiotics and butyric acid producing bacteria and decreasing the abundance of pathogenic bacteria. FDB also regulates the levels of metabolites involved in TCA cycle and amino acid metabolism in urine and small intestine content. Correlation analysis shows that FDB-modulated microbiota is highly associated with its antifatigue effect. FDB may ameliorate fatigue and intestinal injury through targeting small intestinal microbiota.
Promoting Health and Food Literacy through Nutrition Education at Schools: The Italian Experience with MaestraNatura Program
Authors: Scazzocchio Beatrice , Varì Rosaria , d’Amore Antonio , Chiarotti Flavia , Del Papa Sara , Silenzi Annalisa , Gimigliano Annamaria , Giovannini Claudio , Masella Roberta
Published: May 2021

MaestraNatura is an innovative nutrition education program aimed at both enhancing awareness about the importance of a healthy food–lifestyle relationship and the ability to transfer the theoretical principles of nutrition guidelines to everyday life. The educational contents of the program resulted from the analysis of the answers to a questionnaire submitted to students aged 6–13 in order to assess their degree of knowledge about nutritional facts. Educational paths were specifically designed and implemented to address the main knowledge gaps identified through the analysis of the answers and were then tested for teachers’ satisfaction in a sample of 56 schools in the north, centre, and south of Italy, involving 790 classes, 600 teachers, and 15,800 students. The results showed an approval rating from teachers from 90% to 94%. Said paths were designed for primary (6–10 years old) and first-level secondary (11–13 years old) school students. In addition, in a pilot study carried out in nine Educational Institutes located in an area close to Rome (Lazio region), a specific path was tested for effectiveness in increasing students’ knowledge about fruit and vegetables by conducting questionnaires before (T0) and after (T1) the didactic activities. Results showed a significant increase in right answers at T1 with respect to T0 (z = 2.142, p = 0.032). Fisher’s exact probability test showed an answer variability depending on the issue considered. In conclusion, this work could be considered as a first necessary step toward the definition of new educational program, aimed at increasing food literacy and favouring a healthier relationship with food, applicable in a widespread and effective manner, also outside of Italy.
Probiotic and Oxytocin Combination Therapy in Patients with Autism Spectrum Disorder: A Randomized, Double-Blinded, Placebo-Controlled Pilot Trial
Authors: Kong Xue-Jun , Liu Jun , Liu Kevin , Koh Madelyn , Sherman Hannah , Liu Siyu , Tian Ruiyi , Sukijthamapan Piyawat , Wang Jiuju , Fong Michelle , Xu Lei , Clairmont Cullen , Jeong Min-Seo , Li Alice , Lopes Maria , Hagan Veronica , Dutton Tess , Chan Suk-Tak (Phoebe) , Lee Hang , Kendall Amy , Kwong Kenneth , Song Yiqing
Published: May 2021

Autism spectrum disorder (ASD) is a rapidly growing neurodevelopmental disorder. Both probiotics and oxytocin were reported to have therapeutic potential; however, the combination therapy has not yet been studied. We conducted a randomized, double-blinded, placebo-controlled, 2-stage pilot trial in 35 individuals with ASD aged 3–20 years (median = 10.30 years). Subjects were randomly assigned to receive daily Lactobacillus plantarum PS128 probiotic (6 × 1010 CFUs) or a placebo for 28 weeks; starting on week 16, both groups received oxytocin. The primary outcomes measure socio-behavioral severity using the Social Responsiveness Scale (SRS) and Aberrant Behavior Checklist (ABC). The secondary outcomes include measures of the Clinical Global Impression (CGI) scale, fecal microbiome, blood serum inflammatory markers, and oxytocin. All outcomes were compared between the two groups at baseline, 16 weeks, and 28 weeks into treatment. We observed improvements in ABC and SRS scores and significant improvements in CGI-improvement between those receiving probiotics and oxytocin combination therapy compared to those receiving placebo (p < 0.05). A significant number of favorable gut microbiome network hubs were also identified after combination therapy (p < 0.05). The favorable social cognition response of the combination regimen is highly correlated with the abundance of the Eubacterium hallii group. Our findings suggest synergic effects between probiotics PS128 and oxytocin in ASD patients, although further investigation is warranted.
Obesity Development and Signs of Metabolic Abnormalities in Young Göttingen Minipigs Consuming Energy Dense Diets Varying in Carbohydrate Quality
Authors: Curtasu Mihai Victor , Skou Hedemann Mette , Nygaard Lærke Helle , Bach Knudsen Knud Erik
Published: May 2021

Consumption of fructose has been associated with a higher risk of developing obesity and metabolic syndrome (MetS). The aim of this study was to examine the long-term effects of fructose compared to starch from high-amylose maize starch (HiMaize) at ad libitum feeding in a juvenile Göttingen Minipig model with 20% of the diet provided as fructose as a high-risk diet (HR, n = 15) and 20% as HiMaize as a lower-risk control diet (LR, n = 15). The intake of metabolizable energy was on average similar (p = 0.11) among diets despite increased levels of the satiety hormone PYY measured in plasma (p = 0.0005) of the LR pigs. However, after over 20 weeks of ad libitum feeding, no difference between diets was observed in daily weight gain (p = 0.103), and a difference in BW was observed only at the end of the experiment. The ad libitum feeding promoted an obese phenotype over time in both groups with increased plasma levels of glucose (p = 0.005), fructosamine (p < 0.001), insulin (p = 0.03), and HOMA-IR (p = 0.02), whereas the clinical markers of dyslipidemia were unaffected. When compared to the LR diet, fructose did not accelerate the progression of MetS associated parameters and largely failed to change markers that indicate a stimulated de novo lipogenesis.
Enteral Nutrition by Nasogastric Tube in Adult Patients under Palliative Care: A Systematic Review
Authors: Sánchez-Sánchez Eduardo , Ruano-Álvarez María Araceli , Díaz-Jiménez Jara , Díaz Antonio Jesús , Ordonez Francisco Javier
Published: May 2021

Nutritional management of patients under palliative care can lead to ethical issues, especially when Enteral Nutrition (EN) is prescribed by nasogastric tube (NGT). The aim of this review is to know the current status in the management of EN by NG tube in patients under palliative care, and its effect in their wellbeing and quality of life. The following databases were used: PubMed, Web of Science (WOS), Scopus, Scielo, Embase and Medline. After inclusion and exclusion criteria were applied, as well as different qualities screening, a total of three entries were used, published between 2015 and 2020. In total, 403 articles were identified initially, from which three were selected for this review. The use of NGT caused fewer diarrhea episodes and more restrictions than the group that did not use NG tubes. Furthermore, the use of tubes increased attendances to the emergency department, although there was no contrast between NGT and PEG devices. No statistical difference was found between use of tubes (NGT and PEG) or no use, with respect to the treatment of symptoms, level of comfort, and satisfaction at the end of life. Nevertheless, it improved hospital survival compared with other procedures, and differences were found in hospital stays in relation to the use of other probes or devices. Finally, there are not enough quality studies to provide evidence on improving the health status and quality of life of the use of EN through NGT in patients receiving palliative care. For this reason, decision making in this field must be carried out individually, weighing the benefits and damages that they can cause in the quality of life of the patients.
Influence of Human Milk on Very Preterms’ Gut Microbiota and Alkaline Phosphatase Activity
Authors: Morais Juliana , Marques Cláudia , Faria Ana , Teixeira Diana , Barreiros-Mota Inês , Durão Catarina , Araújo João , Ismael Shámila , Brito Sara , Cardoso Manuela , Macedo Israel , Pereira Esmeralda , Tomé Teresa , Calhau Conceição
Published: May 2021

The FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother’s own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC.
Nutritional Support for Bariatric Surgery Patients: The Skin beyond the Fat
Authors: Toninello Paolo , Montanari Alvise , Bassetto Franco , Vindigni Vincenzo , Paoli Antonio
Published: May 2021

Body contouring surgery after the massive weight loss due to bariatric surgery deals with different kinds of complications. The aim of this review is to analyze the role that some nutrients may play in tissue healing after surgery, thus helping plastic surgeons to improve the aesthetic and health outcomes in massive weight loss patients under a multidisciplinary approach. As a matter of fact, preoperative nutritional deficiencies have been shown for vitamins and minerals in a large percentage of post-bariatric patients. Preoperative deficiencies mainly concern iron, zinc, selenium, and vitamins (both fat-soluble and water-soluble), but also total protein. During the postoperative period, these problems may increase because of the patients’ very low intake of vitamins and minerals after bariatric surgery (below 50% of the recommended dietary allowance) and the patients’ low compliance with the suggested multivitamin supplementation (approximately 60%). In the postoperative period, more attention should be given to nutritional aspects in regard to the length of absorptive area and the percentage of weight loss.
Multi-Nutrient Fortified Dairy-Based Drink Reduces Anaemia without Observed Adverse Effects on Gut Microbiota in Anaemic Malnourished Nigerian Toddlers: A Randomised Dose–Response Study
Authors: Owolabi Adedotun J. , Senbanjo Idowu O. , Oshikoya Kazeem A. , Boekhorst Jos , Eijlander Robyn T. , Kortman Guus A. M. , Hageman Jeske H. J. , Samuel Folake , Melse-Boonstra Alida , Schaafsma Anne
Published: May 2021

Prevalence of anaemia among Nigerian toddlers is reported to be high, and may cause significant morbidity, affects brain development and function, and results in weakness and fatigue. Although, iron fortification can reduce anaemia, yet the effect on gut microbiota is unclear. This open-label randomised study in anaemic malnourished Nigerian toddlers aimed to decrease anaemia without affecting pathogenic gut bacteria using a multi-nutrient fortified dairy-based drink. The test product was provided daily in different amounts (200, 400 or 600 mL, supplying 2.24, 4.48 and 6.72 mg of elemental iron, respectively) for 6 months. Haemoglobin, ferritin, and C-reactive protein concentrations were measured to determine anaemia, iron deficiency (ID) and iron deficiency anaemia (IDA) prevalence. Faecal samples were collected to analyse gut microbiota composition. All three dosages reduced anaemia prevalence, to 47%, 27% and 18%, respectively. ID and IDA prevalence was low and did not significantly decrease over time. Regarding gut microbiota, Enterobacteriaceae decreased over time without differences between groups, whereas Bifidobacteriaceae and pathogenic E. coli were not affected. In conclusion, the multi-nutrient fortified dairy-based drink reduced anaemia in a dose-dependent way, without stimulating intestinal potential pathogenic bacteria, and thus appears to be safe and effective in treating anaemia in Nigerian toddlers.
Why Are Spanish Nurses Going to Work Sick? Questionnaire for the Measurement of Presenteeism in Nurses
Authors: Sarabia-Cobo Carmen María , Sáenz-Jalón María , Cabeza-Díaz Pedro , Torres-Manrique Blanca , González-Martínez Obdulio Manuel , Alonso-Jiménez Esperanza , Cantarero-Prieto David , Pascual-Sáez Marta
Published: May 2021

Presenteeism is defined as the presence of the worker at their workplace despite not being in optimal physical or mental conditions. Presenteeism is a phenomenon that has been poorly studied in the context of healthcare. Despite the many negative consequences associated with presenteeism, to date, no studies have investigated this issue in nurses in Spain. The objective was to develop and validate a questionnaire on presenteeism to be used by nursing staff in Spain. Methods: A psychometric study for the development and validation of a questionnaire. The PRESENCA® questionnaire on presenteeism was created by a panel of experts, based on a survey comprised of 31 Likert-type items. Results: In total, 355 nurses completed the questionnaire. The factorial analysis revealed the existence of 3 factors and confirmed appropriate levels of validity and reliability (alpha = 0.729). Conclusions: The PRESENCA® questionnaire is the first tool developed and validated in Spanish for the assessment of presenteeism in nursing. Our findings demonstrate that this scale has appropriate psychometric properties and its use may facilitate the detection of presenteeism among professionals. As a result, use of this questionnaire may contribute towards the improvement of clinical safety.
Occurrence of Legionella spp. in Man-Made Water Sources: Isolates Distribution and Phylogenetic Characterization in the Emilia-Romagna Region
Authors: Mazzotta Marta , Salaris Silvano , Pascale Maria Rosaria , Girolamini Luna , Cristino Sandra
Published: May 2021

Legionella species distribution in the Emilia-Romagna region, involving hospital (H) and community (C) environments, was conducted. Legionella culture, agglutination test, and mip-gene sequencing were applied on 240 isolates. The analysis showed a higher prevalence of non-Legionellapneumophila (n-Lp) species (84.1%) compared with L. pneumophila (Lp) (15.9%), with a higher frequency of n-Lp with respect to Lp species in both environments (77.6% and 96.4%, in H and C, respectively). The Shannon index showed a significant difference in Legionella distribution (p = 0.00017), with a significant abundance of Lp in the H compared with C environment (p = 0.00028). The continuous disinfection treatment in H could contribute to adaptive survival of the Lp species. Phylogenetic analysis revealed a conservative clade distribution between H and C: L. feeleii clade with three subclades in C and the Lp clade with five subclades in H and two in C, respectively. Our findings suggest the importance of Legionella surveillance both in H and C, with a focus on n-Lp species less connected to human disease. The Legionella prevalence and diversity found here indicate that geographical and temporal isolate evolution should be considered during surveillance, particularly in the light of global warming and changes in population risk factors.
Low Seroprevalence of Aichi Virus Infection in Taiwan
Authors: Chen Bao-Chen , Huang Tsi-Shu , Huang Nuan-Ya , Chen Chiao-Shan , Chen Yao-Shen , Chang Tsung-Hsien
Published: May 2021

Aichi virus (AiV) belongs to the genus Kobuvirus of the family Picornaviridae; it is a single-stranded positive-sense RNA virus without an envelope. AiV causes acute gastroenteritis, abdominal pain, nausea, vomiting, and fever. Low incidence and high seroprevalence of AiV infections have been reported in several regions of the world; however, little was known on the prevalence of AiV infections in Taiwan. This study described the first two cases of AiV infection and analyzed AiV seroprevalence in Taiwan. A total of 700 sera were collected from a single hospital in southern Taiwan. The neutralization assay was employed to assess AiV neutralization antibodies in the serum. The test identified 48 positive cases, with a seroprevalence of 6.86%. Results also showed a gradual increase in AiV seroprevalence rate with age. Compared with other countries, Taiwan had a relatively low AiV seroprevalence, suggesting a low incidence of or sporadic AiV infections.