bluuebunny/crossref_metadata_2025 huggingface dataset:
Recommendations for assessing materials criticality
Authors: Lloyd Stafford , Lee Jacquetta , Clifton Andrew , Elghali Lucia , France Chris
Published: October 2012

This paper provides recommendations for assessing the criticality of materials (metals and non-fuel minerals), including the need for context-dependent assessment methods, providing a framework for conducting criticality assessments. Materials criticality captures concerns over the accessibility of materials, as the product of assessing a material’s ‘supply risk’ and the impact of a supply restriction. Through a review of selected studies, problems with criticality assessments are discussed, highlighting how these become particularly important when the results of assessments are used in decision making. Considering how the results of criticality assessments are used in decision making highlights how criticality exhibits some of the characteristics of a ‘complex context’. Building on predefined attributes of effective decision support in complex contexts, recommendations are made on how these problems can be addressed to better assess criticality in the future. These also include building on metric-based assessment methods by developing scenarios of future material supply and demand.
Aboveground–belowground herbivore interactions: a meta‐analysis
Authors: Johnson Scott N. , Clark Katherine E. , Hartley Susan E. , Jones T. Hefin , McKenzie Scott W. , Koricheva Julia
Published: October 2012

Research investigating interactions between aboveground (AG) and belowground (BG) herbivores has been central to characterizing AG–BG linkages in terrestrial ecosystems, with many of these interactions forming the basis of complex food webs spanning the two subsystems. Despite the growing literature on the effects of AG and BG herbivores on each other, underlying patterns have been difficult to identify due to a high degree of context dependency. In this study, we present the first quantitative meta‐analysis of AG and BG herbivore interactions. Previous global predictions, specifically that BG herbivores normally promoted AG herbivore performance and AG herbivores normally reduced BG herbivore performance, were not supported. Instead, the meta‐analysis identified four factors that determined the outcome of AG–BG interactions. (1) Sequence of herbivore arrival on host plants was important, with BG herbivores promoting AG herbivore performance only when introduced to the plant simultaneously, whereas AG herbivores had negative effects on BG herbivores only when introduced first. (2) AG herbivores negatively affected BG herbivore survival but tended to increase population growth rates. (3) AG herbivores negatively affected BG herbivore performance on annual plants, but not on perennials, and these effects were observed more consistently in laboratory than field studies. (4) The type of herbivore was also important, with BG insect herbivores belonging to the order Diptera (i.e., true flies) having the strongest negative effects on AG herbivores. Coleoptera (i.e., beetles) species were the most widely investigated BG herbivores and had positive impacts on AG Homoptera (e.g., aphids), but negative effects on AG Hymenoptera (e.g., sawflies). The strongest negative outcomes for BG herbivores were seen when the AG herbivore was a Coleoptera species. We found no evidence for publication bias in AG–BG herbivore interaction literature and conclude that several biological and experimental factors are important for predicting the outcome of AG–BG herbivore interactions. The sequence of herbivore arrival on the host plant was among the most influential.
Where do seeds go when they go far? Distance and directionality of avian seed dispersal in heterogeneous landscapes
Authors: Carlo Tomás A. , García Daniel , Martínez Daniel , Gleditsch Jason M. , Morales Juan M.
Published: October 2012

Seed dispersal at large scales strongly influences plant population dynamics. Still, ecologists have rarely measured seed dispersal at relevant scales, and the role of habitat types in affecting seed dispersal at long distances remains unexplored. We studied seed dispersal of Ilex aquifolium and Crataegus monogyna in northern Spain, hypothesizing that seeds would be recovered at higher rates and at longer distances (LDD) at habitats with fleshy‐fruited trees, compared to habitats with other tree types or at open habitats. We tracked seeds in eight landscapes by enriching trees with 15N isotopes at the center of landscapes, and then detected 15N‐marked seeds by sampling at distances of up to 700 m. We found that seeds arrive in greater densities and at longer distances in habitats with trees, particularly fleshy‐fruited types, producing different LDD probabilities for each habitat. Results also show a disproportional arrival of seeds in habitats similar to those of mother plants, which should affect seed establishment and the genetic diversity of plant neighborhoods. Findings reveal the strong dependence of seed dispersal on the existing templates that guide the movements of avian dispersers in heterogeneous landscapes and also suggest that LDD above tree lines and beyond hard habitat edges can be difficult.
Elimination of trace organics in an MBR/RO system for water reuse
Authors: De la Torre T. , Rodríguez C. , Alonso E. , Santos J. L. , Malfeito J. J.
Published: October 2012

An intensive programme for detection of trace organics was performed in a membrane bioreactor (MBR) plant in Almuñécar (south of Spain) over 1 year. The compounds investigated included 15 pharmaceutically active compounds, 12 polycyclic aromatic hydrocarbons and eight other compounds (nonylphenols, linear alkylbenzene sulphonates and phthalates). The MBR operated with two lines in parallel using a hollow fibre and a flat sheet membrane respectively. Additionally, a reverse osmosis (RO) plant treated the MBR permeate over 1 month and the elimination of trace organics by the MBR/RO system was assessed. The elimination efficiency of trace organics by the MBR was similar to that found in a conventional activated sludge plant treating the same influent. The concentration of trace organics was reduced after the MBR to a great extent and no significant differences were found between the two lines operating in parallel. The elimination efficiency increased up to 80–100% after passing the RO system. The results indicated that the MBR effluent reached the standard required by the Spanish Royal Decree for Water Reuse and can therefore be reused for multiple purposes, but advanced treatment like RO is necessary when the highest effluent quality is required.
Minimizing risks in wastewater reuse: proposed operational principles and guidelines for South Africa
Authors: Adewumi J. R. , Ilemobade A. A. , van Zyl J. E.
Published: October 2012

Treated wastewater represents a significant potential source of reclaimed water for some beneficial reuses. However, public concern over the risks/health-related hazards of wastewater reuse has limited the general acceptability of reuse systems in many countries. It is important to manage the operation of recycled water systems in such a way that it will not adversely affect public health and the environment. Management of recycled water involves process control and compliance monitoring. This paper presents proposed unit process monitoring guidelines to classify the performances of treatment units according to the pollutant removal efficiencies and frequency of sampling to test for effluent quality suitable for reuse. The paper also highlights the procedure for proper inspection of treatment facilities to ensure optimum performances. It proposes minimum quality requirements for different reuse activities in South Africa such as domestic (toilet flushing, watering private gardens), irrigation (crops, landscape, public parks and golf courses, cemeteries), industrial (system cooling and process water) and other activities (construction works, street cleaning, fire protection, groundwater recharge). By employing the wastewater treatment monitoring and sampling procedures proposed in this paper, health-related hazards can be minimized while public confidence in reuse schemes will be enhanced.
Applicability of domestic grey water reuse for alleviation of water crisis in Dhaka City
Authors: Biswas Shishir Kumar , Rahman Md. Mafizur , Rahman Md. Yeasir Arif , Rahman Md. Mehbuboor
Published: October 2012

Overdependency on ground water resources for meeting the daily water demand in Dhaka City is leading to excessive pressure on ground water aquifer systems and is deepening the ground water table every year. This paper focuses on how reuse of grey water generated from different sources of domestic purposes every day can alter the considerable daily water demand in this city. Sampling and characterization of grey water was accomplished according to different types of residential sources such as water from brushing, bathing, floor washing and laundry. In Dhaka City, household average grey water generation is 85 L per capita per day, which is 60% of the total waste water generated in a house in a day. After reclamation, this grey water can be reused for toilet flushing, gardening, car washing and fire fighting, which can play a crucial role of meeting city water demand since reusable grey water generation is around two times greater than the total demand of water for those purposes. During characterization, emphasis was given to the determination of basic parameters like pH, turbidity, colour, solid content, bio-chemical oxygen demand (BOD), chemical oxygen demand (COD), total coliform (TC) and faecal coliform (FC). Among all the grey water sources, laundry water was found to be the most contaminated. Aesthetic quality of grey water is another issue of public acceptance.
A Clinical Audit on Diabetes Care in Patients with Type 2 Diabetes in Al-Ain, United Arab Emirates
Authors: Shehab Abdulla , Elnour Asim , Abdulle Abdishakur
Published: October 2012

Objectives:

To implement a prospective interventional clinical audit to evaluate the current clinical practice and the effect of standard interventions on the management of type 2 diabetes (T2DM).

Methods:

254 patients with T2DM where recruited in a specialized diabetes care center in Al-Ain, UAE. The diabetes care components were audited before (baseline) and after (3 and 6 months) implementation of Institute of Clinical System Improvement (ICSI) guidelines. Data was compared against international guidelines to achieve target goals of normoglycemia, blood pressure (BP), and low density lipoprotein-cholesterol (LDL-C). We measured changes in mean scores of patient satisfaction level regarding diabetes care at similar intervals, by validated Patient Satisfaction Questionnaire (PSQ-18).

Results:

We observed a significant reduction in fasting blood glucose (FBG; mean± SD; 9.3 ± 0.03 vs 7.4 ± 0.3mmol/l; P=0.03), and HbA1c (8.7 ± 0.02 vs 8.1 ± 0.02 %; P=0.04) levels after 6 months compared with baseline. Patients who achieved target FBG and HbA1c levels improved significantly (45.7 vs 81.1%; P=0.03), and (40.1 vs 73.6%; P=0.04), respectively. The LDL-C levels improved, though this was not statistically significant. Patients achieving target of BP control improved significantly (SBP 142±7.6 and DBP 95±6.2 vs SBP 136±8.2 and DBP 87±5.8 mmHg;P=0.05).

Conclusions:

The results of this interventional audit were generally positive and emphasized the feasibility of improving the current clinical practice. Our individualized approach has helped us to achieve a better target in glycemic and BP control as well as patient satisfaction. Further research is needed to understand the long-term impact of our structured approach to improve the quality of T2DM care in the UAE.
Entrapment of ethyl vanillin in calcium alginate and calcium alginate/poly(vinyl alcohol) beads
Authors: Levic Steva , Djordjevic Verica , Rajic Nevenka , Milivojevic Milan , Bugarski Branko , Nedovic Viktor
Published: October 2012

Electrostatic extrusion was applied to the encapsulation of 3-ethoxy-4-hydroxybenzaldehyde (ethyl vanillin) in calcium alginate and calcium alginate/poly(vinyl alcohol) beads. The calcium alginate/poly(vinyl alcohol) hydrogel spheres were formed after contact with the cross-linker solution of calcium chloride, followed by the freeze-thaw method for poly(vinyl alcohol) gel formation. The entrapment of aroma in beads was investigated by FTIR and thermal analysis (thermogravimetry/differential thermal gravimetry; TGA/DTG). The mass loss in the temperature range of 150–300°C is related to degradation of the matrix and the release of ethyl vanillin. According to the DTG curve, the release of ethyl vanillin occurs at about 260°C. TGA measurements of the stored samples confirmed that formulations were stable for a period of one month. FTIR analysis provides no evidence for chemical interactions between flavour and alginate that would alter the nature of the functional groups in the flavour compound.
A new phenanthroindolizidine alkaloid from Tylophora indica
Authors: Dhiman Mini , Khanna Amit , Manju Sreedharannair
Published: October 2012

A new phenanthroindolizidine alkaloid, 3-O-demethyl tylophorinidine (VI), was isolated from the aerial (leaves and stem) parts of Tylophora indica and characterized using different spectral techniques. Gel chromatography and reverse phase preparative HPLC were used for sample purification. The new alkaloid, VI, was screened for anticancer activity against a panel of different cancer cell lines and it showed significant anticancer activity with IC50 value in the range of 0.89–1.40 μM.
Curricular Coherence and the Common Core State Standards for Mathematics
Authors: Schmidt William H. , Houang Richard T.
Published: October 2012

In this work, we explored the relationship of the Common Core State Standards in Mathematics (CCSSM) to student achievement. Building on techniques developed for the Third International Mathematics and Science Study (TIMSS), we found a very high degree of similarity between CCSSM and the standards of the highest-achieving nations on the 1995 TIMSS. A similar analysis revealed wide variation in the proximity of state standards in effect in 2009 to the CCSSM. Finally, we used regression and analysis of covariance techniques to assess the relationship between the proximity of a state’s standards to the CCSSM and performance on the 2009 National Assessment of Educational Progress (NAEP). After adjusting for cut-points on state assessments and controlling for state demographics related to socioeconomic status and poverty, we found that states with standards more like the CCSSM, on average, had higher NAEP scores.
Helicopter electromagnetic data map ice thickness at Mount Adams and Mount Baker, Washington, USA
Authors: Finn Carol A. , Deszcz-Pan Maria , Bedrosian Paul A.
Published: October 2012

Ice-thickness measurements critical for flood and mudflow hazard studies are very sparse on Cascade Range (North America) volcanoes. Helicopter electromagnetic (HEM) data collected to detect hydrothermal alteration are used to determine ice thickness over portions of Mount Baker and Mount Adams volcanoes. A laterally continuous inversion method provides good estimates of ice <100 m thick over water-saturated and altered regions where the resistivity of the basement is <200 Ωm. For areas with ice overlying fresh, resistive rocks with small resistivity contrasts between ice and rock, ice thickness is not well resolved. The ice thicknesses derived from HEM data are consistent with the previous drillhole data from Mount Adams and radar data from both volcanoes, with mean thicknesses of 57 m for Mount Adams and 68 m for Mount Baker. The thickest ice on Mount Baker rests on the gentle lower slopes whereas the thickest ice at Mount Adams lies on the flat summit. Ice volume calculations suggest that Mount Baker contains ∽710 × 106 m3 of ice in the HEM survey area, with a crude estimate of ∽1800 × 106m3 for the entire volcano. Ice volume on Mount Adams is 65 × 106m3 in parts of the HEM survey area and ∽200 × 106m3 overall.
Chemical analysis of ice vein microenvironments: II. Analysis of glacial samples from Greenland and Antarctica
Authors: Barletta Robert E. , Priscu John C. , Mader Heidy M. , Jones Warren L. , Roe Christopher H.
Published: October 2012

Chemical constituents trapped within glacial ice provide a unique record of climate, as well as repositories for biological material such as pollen grains, fungal spores, viruses, bacteria and dissolved organic carbon. Past research suggests that the veins of polycrystalline ice may provide a liquid microenvironment for active microbial metabolism fueled by concentrated impurities in the veins. Despite these claims, no direct measurements of impurity concentration in ice veins have been made. Using micro-Raman spectroscopy, we show that sulfate and nitrate concentrations in the veins of glacial ice from Greenland (Greenland Ice Sheet Project 2) and Antarctic (Newall Glacier and a Dominion Range glacier) core samples were 104 and 105 times greater than the concentrations measured in melted (bulk) core water. Methanesulfonate was not found in the veins, consistent with its presence as particulate matter within the ice. The measured vein concentration of molecular anions implies a highly acidic (pH < 3) vein environment with high ionic strength (mM-M). We estimate that the vein volume provides 16.7 and 576 km3 of habitable space within the Greenland and Antarctic ice sheets, respectively, which could support the metabolism of organisms that are capable of growing in cold, high ionic strength solutions with low pH.
The Incidence, Predisposing Factors, Complications and Outcome of Preeclampsia in Diabetic Pregnancy
Authors: Jesmin S , Jahan S , Khan MI , Sultana N , Jerin J , Habib SH , Paul D
Published: October 2012

Introduction: Preeclampsia is a serious complication of pregnancy and common cause of fetal and maternal morbidity as well as mortality worldwide. In diabetic women, the chance of preeclampsia is increased. The incidence of preeclampsia in diabetic pregnancy is approximately 10 to 15 percent, which is associated with poor glycaemic control. Aim: This study was carried out to find the predisposing factors related to preeclampsia and determine the complications of preeclampsia in diabetic pregnancy and also the impact of preeclampsia in infants born to diabetic mothers. Methods: This prospective study was carried out at the Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka. The Patient population consisted of 80 diabetic pregnant women who attended or admitted to BIRDEM hospital during the study period. The women were divided into groups: 50 pregnant diabetic women with preeclampsia were taken as case. 30 pregnant diabetic women without preeclampsia were taken as control. Diagnosis of preeclampsia was made on the basis of the criteria of the Committee on Terminology of the American College of Obstetrician and Gynecologist. Results: Preterm delivery (<37 weeks gestation) was higher among study group (64%) compared to control (33.3%) women. Term delivery was 36.0 vs 66.7 percent among case and control women, respectively. The distribution is statistically significant (P<0.01). 35 percent of Caesarean section was done due to fetal distress in the study group and in control group it was 20 percent. In study group, 22.5 percent Caesarean sections were done due to impending eclampsia and eclampsia, 705 percent due to accidental haemorrhage and 5 percent due to IUGR. Maternal complication in study and control subjects. In the case group, maximum number of the women (16%) showed signs of impending eclampsia, while among control women, maximum number (10%) developed postpartum haemorrhage (PPH). 48 percent neonates were of low birth weight and in controls it was 13.3 percent. Both hyperbilirubinaemia (40%) and hypoglycaemia (30%) were more in study group than controls (16.66% and 20%, respectively). Perinatal outcome among study group and controls. Neonatal survival was 82.0 percent in study group and 86.7 percent in control group. Comparison of Perinatal outcome between the groups is not statistically significant. Most of the perinatal mortality was due to prematurity (8%) and intrauterine death (6%). In control group, most of the perinatal deaths were due to congenital anomalies (6.6%). Conclusion: The higher incidence among study group may be, in part, the result of more preterm birth or shortened gestational duration because early delivery is a consequence of preeclampsia. The higher rate in associated with preeclampsia was due to increased incidence of IUD and prematurity. DOI: http://dx.doi.org/10.3329/birdem.v1i1.12379 Birdem Med J 2011; 1(1): 10-14
Clinical Spectrum and Management of Diabetic Ketoacidosis: Experience in A Tertiary Care Hospital
Authors: Rahim MA , Uddin KN , Zaman S , Musa AKM , Rahman MR , Hossain MD , Ahmed AKMS , Ahmed JU , Samad T , Haque HF , Dewan P , Sarkar RSC , Dastidar S
Published: October 2012

Background: Diabetic ketoacidosis (DKA) is an acute metabolic complication of diabetes mellitus (DM). It may be the presenting feature in type 1 DM, but more commonly it complicates previously diagnosed diabetic patients, both type 1 and type 2. If not recognized early and treated in a judicious way the outcome is often fatal. Objectives: The objectives of this study was to see the common presenting features of DKA, their precipitating causes, patterns of electrolyte imbalance, treatment requirement in early hours and to see the outcome. Materials and methods: This cross sectional study was done in BIRDEM General Hospital on fifty adult patients who presented with DKA over a period of nine months (January 2007 to September 2007). Results: Total number of patients were 50, male were 24 and female were 26 (M:F =12:13). Mean age was 27.6 ± 3.7 years. The incidence of DKA was more in known diabetic patients (32, 64%), in comparison with new cases (18, 36%). Frequency was more in poor village people (31, 62%). Vomiting (24, 48%) was the most frequent complaint, followed by fever (19, 38%), nausea (16, 32%), abdominal pain (14, 28%), weakness (13, 26%), polyuria (12, 24%) and polydypsia ( 8, 16%). Infection (18, 36%) was the most common precipitating cause, closely followed by inadherence to insulin therapy (17, 34%). In 12 (24%) cases no cause could be identified. Glycaemic control was poor, HbA1c was >7% in 98% cases. Severe acidosis (pH < 7) was less common (4, 8%) and gross electrolyte imbalance was uncommon but all patients required potassium supplementation in course of treatment. Neutrophilic leukocytosis was present in 44 (88%) cases, irrespective of presence of infection. Mortality was low (3, 6%). Conclusion: Diagnosis and treatment of DKA is not difficult if recognized early. So, high index of suspicion is necessary, particularly in previously undiagnosed cases. DOI: http://dx.doi.org/10.3329/birdem.v1i1.12380 Birdem Med J 2011; 1(1): 15-20
Surgical Management of Calcific Metamorphosis of Pulp: A Case Report
Authors: Kulsum U , Farzana F
Published: October 2012

A case is reported which states calcific metamorphosis of pulp in a mandibular left lateral incisor and concomitant pulp necrosis of both central incisors for a single trauma. There was a large periapical lesion associated with the teeth. Conventional endodontic treatment was performed on teeth with pulp necrosis, but the canal with calcific metamorphosis could not be negotiated with endodontic files. The periapical lesion was surgically removed and diagnosed as acute periapical abscess on histopathology. During the surgical procedure root apices of calcified tooth  as well as other teeth were removed and retrograde cavity was prepared and filled with glass ionomer cement i.e apicectomy was done. The bony defect was filled with calcium hydroxyappatite crystal. Six months later the teeth were asymptomatic and radiological follow-up showed gradual healing of the bony cavity. DOI: http://dx.doi.org/10.3329/birdem.v1i1.12396 Birdem Med J 2011; 1(1): 46-50
BIRDEM News Vol.1(1)
Authors: Editor The
Published: October 2012

DOI: http://dx.doi.org/10.3329/birdem.v1i1.12399 Birdem Med J 2011; 1(1): 56
Changes in Cis-regulatory Elements during Morphological Evolution
Authors: Gaunt Stephen , Paul Yu-Lee
Published: October 2012

How have animals evolved new body designs (morphological evolution)? This requires explanations both for simple morphological changes, such as differences in pigmentation and hair patterns between different Drosophila populations and species, and also for more complex changes, such as differences in the forelimbs of mice and bats, and the necks of amphibians and reptiles. The genetic changes and pathways involved in these evolutionary steps require identification. Many, though not all, of these events occur by changes in cis-regulatory (enhancer) elements within developmental genes. Enhancers are modular, each affecting expression in only one or a few tissues. Therefore it is possible to add, remove or alter an enhancer without producing changes in multiple tissues, and thereby avoid widespread (pleiotropic) deleterious effects. Ideally, for a given step in morphological evolution it is necessary to identify (i) the change in phenotype, (ii) the changes in gene expression, (iii) the DNA region, enhancer or otherwise, affected, (iv) the mutation involved, (v) the nature of the transcription or other factors that bind to this site. In practice these data are incomplete for most of the published studies upon morphological evolution. Here, the investigations are categorized according to how far these analyses have proceeded.
The Population Genomics of Sunflowers and Genomic Determinants of Protein Evolution Revealed by RNAseq
Authors: Renaut Sébastien , Grassa Christopher J. , Moyers Brook T. , Kane Nolan C. , Rieseberg Loren H.
Published: October 2012

Few studies have investigated the causes of evolutionary rate variation among plant nuclear genes, especially in recently diverged species still capable of hybridizing in the wild. The recent advent of Next Generation Sequencing (NGS) permits investigation of genome wide rates of protein evolution and the role of selection in generating and maintaining divergence. Here, we use individual whole-transcriptome sequencing (RNAseq) to refine our understanding of the population genomics of wild species of sunflowers (Helianthus spp.) and the factors that affect rates of protein evolution. We aligned 35 GB of transcriptome sequencing data and identified 433,257 polymorphic sites (SNPs) in a reference transcriptome comprising 16,312 genes. Using SNP markers, we identified strong population clustering largely corresponding to the three species analyzed here (Helianthus annuus, H. petiolaris, H. debilis), with one distinct early generation hybrid. Then, we calculated the proportions of adaptive substitution fixed by selection (alpha) and identified gene ontology categories with elevated values of alpha. The “response to biotic stimulus” category had the highest mean alpha across the three interspecific comparisons, implying that natural selection imposed by other organisms plays an important role in driving protein evolution in wild sunflowers. Finally, we examined the relationship between protein evolution (dN/dS ratio) and several genomic factors predicted to co-vary with protein evolution (gene expression level, divergence and specificity, genetic divergence [FST], and nucleotide diversity pi). We find that variation in rates of protein divergence was correlated with gene expression level and specificity, consistent with results from a broad range of taxa and timescales. This would in turn imply that these factors govern protein evolution both at a microevolutionary and macroevolutionary timescale. Our results contribute to a general understanding of the determinants of rates of protein evolution and the impact of selection on patterns of polymorphism and divergence.
The Completed Self: An Immunological View of the Human-Microbiome Superorganism and Risk of Chronic Diseases
Authors: Dietert Rodney , Dietert Janice
Published: October 2012

In this review, we discuss an immunological-driven sign termed the Completed Self, which is related to a holistic determination of health vs. disease. This sign (human plus commensal microbiota) forms the human superorganism. The worldwide emergence of an epidemic of chronic diseases has caused increased healthcare costs, increased premature mortality and reduced quality of life for a majority of the world’s population. In addition, it has raised questions concerning the interactions between humans and their environment and potential imbalances. Misregulated inflammation, a host defense-homeostasis disorder, appears to be a key biomarker connecting a majority of chronic diseases. We consider the apparent contributors to this disorder that promote a web of interlinked comorbid conditions. Three key events are suggested to play a role: (1) altered epigenetic programming (AEP) that may span multiple generations, (2) developmental immunotoxicity (DIT), and (3) failure to adequately incorporate commensal microbes as a newborn (i.e., the incomplete self). We discuss how these three events can combine to determine whether the human superorganism is able to adequately and completely form during early childhood. We also discuss how corruption of this event can affect the risk of later-life diseases.
Unconventional Algorithms: Complementarity of Axiomatics and Construction
Authors: Dodig Crnkovic Gordana , Burgin Mark
Published: October 2012

In this paper, we analyze axiomatic and constructive issues of unconventional computations from a methodological and philosophical point of view. We explain how the new models of algorithms and unconventional computations change the algorithmic universe, making it open and allowing increased flexibility and expressive power that augment creativity. At the same time, the greater power of new types of algorithms also results in the greater complexity of the algorithmic universe, transforming it into the algorithmic multiverse and demanding new tools for its study. That is why we analyze new powerful tools brought forth by local mathematics, local logics, logical varieties and the axiomatic theory of algorithms, automata and computation. We demonstrate how these new tools allow efficient navigation in the algorithmic multiverse. Further work includes study of natural computation by unconventional algorithms and constructive approaches.
Concrete Open-Wall Systems Wrapped with FRP under Torsional Loads
Authors: Mancusi Geminiano , Feo Luciano , Berardi Valentino P.
Published: October 2012

The static behavior of reinforced concrete (RC) beams plated with layers of fiber-reinforced composite material (FRP) is widely investigated in current literature, which deals with both its numerical modeling as well as experiments. Scientific interest in this topic is explained by the increasing widespread use of composite materials in retrofitting techniques, as well as the consolidation and upgrading of existing reinforced concrete elements to new service conditions. The effectiveness of these techniques is typically influenced by the debonding of the FRP at the interface with concrete, where the transfer of stresses occurs from one element (RC member) to the other (FRP strengthening). In fact, the activation of the well-known premature failure modes can be regarded as a consequence of high peak values of the interfacial interactions. Until now, typical applications of FRP structural plating have included cases of flexural or shear-flexural strengthening. Within this context, the present study aims at extending the investigation to the case of wall-systems with open cross-section under torsional loads. It includes the results of some numerical analyses carried out by means of a finite element approximation.
A Decolorization Technique with Spent “Greek Coffee” Grounds as Zero-Cost Adsorbents for Industrial Textile Wastewaters
Authors: Kyzas George
Published: October 2012

In this study, the decolorization of industrial textile wastewaters was studied in batch mode using spent “Greek coffee” grounds (COF) as low-cost adsorbents. In this attempt, there is a cost-saving potential given that there was no further modification of COF (just washed with distilled water to remove dirt and color, then dried in an oven). Furthermore, tests were realized both in synthetic and real textile wastewaters for comparative reasons. The optimum pH of adsorption was acidic (pH = 2) for synthetic effluents, while experiments in free pH (non-adjusted) were carried out for real effluents. Equilibrium data were fitted to the Langmuir, Freundlich and Langmuir-Freundlich (L-F) models. The calculated maximum adsorption capacities (Qmax) for total dye (reactive) removal at 25 °C was 241 mg/g (pH = 2) and 179 mg/g (pH = 10). Thermodynamic parameters were also calculated (ΔH0, ΔG0, ΔS0). Kinetic data were fitted to the pseudo-first, -second and -third order model. The optimum pH for desorption was determined, in line with desorption and reuse analysis. Experiments dealing the increase of mass of adsorbent showed a strong increase in total dye removal.
Improved Antileishmanial Activity of Dppz through Complexation with Antimony(III) and Bismuth(III): Investigation of the Role of the Metal
Authors: Lizarazo-Jaimes Edgar , Monte-Neto Rubens , Reis Priscila , Fernandes Nelson , Speziali Nivaldo , Melo Maria , Frézard Frédéric , Demicheli Cynthia
Published: October 2012

Two novel trivalent antimony(III) and bismuth(III) complexes with the nitrogen-donor heterocyclic ligand dipyrido[3,2-a:2',3'-c]phenazine (dppz) were synthesized and characterized as [Sb(dppz)Cl3]∙H2O∙CH3OH and [Bi(dppz)Cl3]. The crystal structure of Sb(III) complex was determined by X-ray crystallography. These complexes were evaluated for their activity against the promastigote form of Sb(III)-sensitive and –resistant Leishmania infantum chagasi and Leishmania amazonensis strains. Both complexes were more effective than dppz alone in inhibiting the growth of Leishmania promastigotes and were at least 77 and 2,400 times more active than potassium antimonyl tartrate in Sb(III)-sensitive and -resistant Leishmania, respectively. The cytotoxicity of dppz and its complexes against mouse peritoneal macrophages occurred at dppz concentrations at least 6-fold greater than those found to be active against Leishmania promastigotes.To investigate the role of the metal in the improved antileishmanial activity of dppz, the activity of the Sb(III) complex was compared between the Sb-resistant mutants and their respective parental sensitive strains. The lack of cross-resistance to the Sb(III)-dppz complex together with the much lower activity of antimonyl tartrate, SbCl3 and BiCl3 strongly support the model that the metal is not active by itself but improves the activity of dppz through complexation.
Two Novel Phenolic Compounds from the Rhizomes of Cyperus rotundus L.
Authors: Zhou Zhongliu , Yin Wenqing
Published: October 2012

Two novel compounds, 1α-methoxy-3β-hydroxy-4α-(3′,4′-dihydroxyphenyl)-1, 2,3,4-tetrahydronaphthalin (1) and 1α,3β-dihydroxy-4α-(3′,4′-dihydroxyphenyl)-1,2,3,4-tetrahydronaphthalin (2), were isolated along with six known compounds 3–8 from the rhizomes of Cyperus rotundus. This paper reports the isolation and full spectroscopic characterization of these new compounds by NMR, UV, IR and MS data.
Dietary Patterns Are Associated with Cognition among Older People with Mild Cognitive Impairment
Authors: Torres Susan J. , Lautenschlager Nicola T. , Wattanapenpaiboon Naiyana , Greenop Kathryn R. , Beer Christopher , Flicker Leon , Alfonso Helman , Nowson Caryl A.
Published: October 2012

There has been increasing interest in the influence of diet on cognition in the elderly. This study examined the cross-sectional association between dietary patterns and cognition in a sample of 249 people aged 65–90 years with mild cognitive impairment (MCI). Two dietary patterns; whole and processed food; were identified using factor analysis from a 107-item; self-completed Food Frequency Questionnaire. Logistic regression analyses showed that participants in the highest tertile of the processed food pattern score were more likely to have poorer cognitive functioning; in the lowest tertile of executive function (OR 2.55; 95% CI: 1.08–6.03); as assessed by the Cambridge Cognitive Examination. In a group of older people with MCI; a diet high in processed foods was associated with some level of cognitive impairment.
Metabolism and Pharmacokinetics of the Anti-Tuberculosis Drug Ethionamide in a Flavin-Containing Monooxygenase Null Mouse
Authors: Palmer Amy L. , Leykam Virginia L. , Larkin Andrew , Krueger Sharon K. , Phillips Ian R. , Shephard Elizabeth A. , Williams David E.
Published: October 2012

Multiple drug resistance (MDR) in Mycobacterium tuberculosis (mTB), the causative agent for tuberculosis (TB), has led to increased use of second-line drugs, including ethionamide (ETA). ETA is a prodrug bioactivated by mycobacterial and mammalian flavin-containing monooxygenases (FMOs). FMO2 is the major isoform in the lungs of most mammals, including primates. In humans a polymorphism exists in the expression of FMO2. FMO2.2 (truncated, inactive) protein is produced by the common allele, while the ancestral allele, encoding active FMO2.1, has been documented only in individuals of African and Hispanic origin, at an incidence of up to 50% and 7%, respectively. We hypothesized that FMO2 variability in TB-infected individuals would yield differences in concentrations and ratios of ETA prodrug and metabolites. In this study we assessed the impact of the FMO2 genetic polymorphism on the pharmacokinetics of ETA after administration of a single oral dose of ETA (125 mg/kg) to wild type and triple Fmo1/2/4-null mice, measuring levels of prodrug vs. metabolites in plasma collected from 0 to 3.5 h post-gavage. All mice metabolized ETA to ETA S-oxide (ETASO) and 2-ethyl-4-amidopyridine (ETAA). Wild type mice had higher plasma concentrations of metabolites than of parent compound (p = 0.001). In contrast, Fmo1/2/4-null mice had higher plasma concentrations of parent compound than of metabolites (p = 0.0001). Thus, the human FMO2 genotype could impact the therapeutic efficacy and/or toxicity of ETA.
The Embodied Life Course: Post-ageism or the Renaturalization of Gender?
Authors: Marshall Barbara , Katz Stephen
Published: October 2012

This paper argues that the sociology of the body must take more account of embodiment as an ongoing process that occurs over the life course, and it suggests that a critical perspective is required that emphasizes the material processes of embodiment by which physical changes in age and time are culturally mediated. We take the concept of the embodied life course as a starting point for probing the temporal aspects of bodily life, for exploring the ways in which biological, biographical and socio-historical time intersect, and for grasping the ways that temporality is materialized and mobilized through bodies. Taking the example of the biomedical reconfiguration of sexual function across the life course, we demonstrate how aging bodies have been opened to new forms of intervention that situate them within new understandings of nature and culture. Conclusions reflect on the contradictions of ‘post-ageist’ discourses and practices that promise to liberate bodies from chronological age, while simultaneously re-naturalizing gender in sexed bodies.
Toxigenic Potential of Aspergillus Species Occurring on Maize Kernels from Two Agro-Ecological Zones in Kenya
Authors: Okoth Sheila , Nyongesa Beatrice , Ayugi Vincent , Kang'ethe Erastus , Korhonen Hannu , Joutsjoki Vesa
Published: October 2012

Two agro-ecological zones in Kenya were selected to compare the distribution in maize of Aspergillus spp. and their toxigenicity. These were Nandi County, which is the main maize growing region in the country but where no human aflatoxicoses have been reported, and Makueni County where most of the aflatoxicosis cases have occurred. Two hundred and fifty-five households were sampled in Nandi and 258 in Makueni, and Aspergillus was isolated from maize. Aspergillus flavus and A. parasiticus isolates were tested for the presence of aflD and aflQ genes. Positive strains were induced to produce aflatoxins on yeast extract sucrose and quantified using liquid chromatography-tandem mass spectrometry (LCMSMS). Aspergillus flavus was the most common contaminant, and the incidence of occurrence in Nandi and Makueni was not significantly different (82.33% and 73.26%, respectively). Toxigenic strains were more prevalent than non-toxigenic strains. All the toxigenic strains from Makueni were of the S-type while those from Nandi belonged to the l-type. Quantitative differences in aflatoxin production in vitro between isolates and between strains were detected with S strains producing relatively larger amounts of total aflatoxins, B toxins and lower values for G toxins. This was in accord with the frequent aflatoxicosis outbreaks in Makueni. However some L strains produced considerable amounts of B toxins. Given the widespread distribution of toxigenic strains in both regions, the risk of aflatoxin poisoning is high when favorable conditions for toxin production occur.
Current Understanding on Aflatoxin Biosynthesis and Future Perspective in Reducing Aflatoxin Contamination
Authors: Yu Jiujiang
Published: October 2012

Traditional molecular techniques have been used in research in discovering the genes and enzymes that are involved in aflatoxin formation and genetic regulation. We cloned most, if not all, of the aflatoxin pathway genes. A consensus gene cluster for aflatoxin biosynthesis was discovered in 2005. The factors that affect aflatoxin formation have been studied. In this report, the author summarized the current status of research progress and future possibilities that may be used for solving aflatoxin contamination.
Human Cytomegalovirus Encoded Homologs of Cytokines, Chemokines and their Receptors: Roles in Immunomodulation
Authors: McSharry Brian P. , Avdic Selmir , Slobedman Barry
Published: October 2012

Human cytomegalovirus (HCMV), the largest human herpesvirus, infects a majority of the world’s population. Like all herpesviruses, following primary productive infection, HCMV establishes a life-long latent infection, from which it can reactivate years later to produce new, infectious virus. Despite the presence of a massive and sustained anti-HCMV immune response, productively infected individuals can shed virus for extended periods of time, and once latent infection is established, it is never cleared from the host. It has been proposed that HCMV must therefore encode functions which help to evade immune mediated clearance during productive virus replication and latency. Molecular mimicry is a strategy used by many viruses to subvert and regulate anti-viral immunity and HCMV has hijacked/developed a range of functions that imitate host encoded immunomodulatory proteins. This review will focus on the HCMV encoded homologs of cellular cytokines/chemokines and their receptors, with an emphasis on how these virus encoded homologs may facilitate viral evasion of immune clearance.
Niemann-Pick C1 (NPC1)/NPC1-like1 Chimeras Define Sequences Critical for NPC1’s Function as a Filovirus Entry Receptor
Authors: Krishnan Anuja , Miller Emily , Herbert Andrew , Ng Melinda , Ndungo Esther , Whelan Sean , Dye John , Chandran Kartik
Published: October 2012

We recently demonstrated that Niemann-Pick C1 (NPC1), a ubiquitous 13-pass cellular membrane protein involved in lysosomal cholesterol transport, is a critical entry receptor for filoviruses. Here we show that Niemann-Pick C1-like1 (NPC1L1), an NPC1 paralog and hepatitis C virus entry factor, lacks filovirus receptor activity. We exploited the structural similarity between NPC1 and NPC1L1 to construct and analyze a panel of chimeras in which NPC1L1 sequences were replaced with cognate sequences from NPC1. Only one chimera, NPC1L1 containing the second luminal domain (C) of NPC1 in place of its own, bound to the viral glycoprotein, GP. This engineered protein mediated authentic filovirus infection nearly as well as wild-type NPC1, and more efficiently than did a minimal NPC1 domain C-based receptor recently described by us. A reciprocal chimera, NPC1 containing NPC1L1’s domain C, was completely inactive. Remarkably, an intra-domain NPC1L1-NPC1 chimera bearing only a ~130-amino acid N–terminal region of NPC1 domain C could confer substantial viral receptor activity on NPC1L1. Taken together, these findings account for the failure of NPC1L1 to serve as a filovirus receptor, highlight the central role of the luminal domain C of NPC1 in filovirus entry, and reveal the direct involvement of N–terminal domain C sequences in NPC1’s function as a filovirus receptor.
A Randomized Comparison of Second-Line Lopinavir/ Ritonavir Monotherapy versus Tenofovir/Lamivudine/ Lopinavir/Ritonavir in Patients Failing Nnrti Regimens: The HIV Star Study
Authors: Bunupuradah Torsak , Chetchotisakd Ploenchan , Ananworanich Jintanat , Munsakul Warangkana , Jirajariyavej Supunnee , Kantipong Pacharee , Prasithsirikul Wisit , Sungkanuparph Somnuek , Bowonwatanuwong Chureeratana , Klinbuayaem Virat , Kerr Stephen J , Sophonphan Jiratchaya , Bhakeecheep Sorakij , Hirschel Bernard , Ruxrungtham Kiat ,   the HIV STAR Study Group
Published: October 2012

Background

Data informing the use of boosted protease inhibitor (PI) monotherapy as second-line treatment are limited. There are also no randomized trials addressing treatment options after failing first-line non-nucleoside reverse transcriptase inhibitor (NNRTI)-regimens.

Methods

HIV-infected subjects ≥18 years, with HIV RNA≥1,000 copies/ml while using NNRTI plus 2 NRTIs, and naive to PIs were randomized to lopinavir/ritonavir (LPV/r) 400/100 mg twice daily monotherapy (mono-LPV/r) or tenofovir disoproxil fumarate (TDF) once daily plus lamivudine (3TC) twice daily plus LPV/r 400/100 mg twice daily (TDF/3TC/LPV/r) at nine sites in Thailand. The primary outcome was time-weighted area under curve (TWAUC) change in HIV RNA over 48 weeks. The a priori hypothesis was that the mono-LPV/r arm would be considered non-inferior if the upper 95% confidence limit in TWAUC mean difference was ≤0.5 log10 copies/ml.

Results

The intention-to-treat (ITT) population comprised 195 patients (mono-LPV/r n=98 and TDF/3TC/LPV/r n=97): male 58%, baseline mean (sd) age of 38 (7) years, CD4+ T-cell count of 204 (135) cells/mm3 and HIV RNA of 4.1 (0.6) log10 copies/ml. The majority had HIV-1 recombinant CRF01_AE infection, and thymidine analogue mutation (TAM)-2 was 3x more common than TAM-1. At 48 weeks, the difference in TWAUC HIV RNA between arms was 0.15 (95% CI -0.04, 0.33) log10 copies/ml, consistent with our definition of non-inferiority. However, the proportion with HIV RNA<50 copies/ml was significantly lower in the mono-LPV/r arm: 61% versus 83% (ITT, P<0.01). Baseline HIV RNA≥5 log10 copies/ml ( P<0.001) and mono-LPV/r use ( P =0.003) were predictors of virological failure. Baseline genotypic sensitivity scores ≥2 and TAM-2 were associated with better virological control in subjects treated with the TDF-containing regimen.

Conclusions

In PI-naive patients failing NNRTI-based first-line HAART, mono-LPV/r had a significantly lower proportion of patients with HIV RNA<50 copies/ml compared to the TDF/3TC/LPV/r treatment. Thus, mono-LPV/r should not be recommended as a second-line option.
Le partage de la vision dans les très petites entreprises
Authors: Filion Louis Jacques , Lima Edmilson
Published: October 2012

Résumé Pour les dirigeants de TPE, faire partager leur vision à toutes les parties prenantes, internes comme externes à l’entreprise, est un enjeu fort. La vision et son partage constituent en effet des outils de premier choix pour amorcer et maintenir vivant le processus de communication et d’apprentissage inhérent aux organisations apprenantes que sont les entreprises. Or il ne peut y avoir vision partagée si certaines conditions préalables ne sont pas réunies. Les auteurs en discernent quatre qui sont les valeurs, la connaissance du secteur, le respect des personnes et l’écoute. Par la suíte, la “conversation stratégique” est le moyen d’affiner et de perfectionner sans cesse la vision et de facilier son appropriation.