bluuebunny/crossref_metadata_2025 huggingface dataset:
Guided meditation for vision acuity training on adolescent myopia: study protocol for an open-label, prospective, multicenter, randomized controlled trial
Authors: Li Yibo , Zhu Lili , Wang Raoying , Yang Xingyue , Jiang Xinqi , Lu Tao
Published: January 2022

Background

Currently, the population with myopia climbs steadily, and is developing toward younger age, posing a great concern to the health of adolescents. Myopia in severe cases can cause irreversible consequences such as glaucoma, blindness, and other complications. At present, the solutions for myopia are glasses, medication, and surgery. This study aims to investigate the role of a physiotherapy category based on guided meditation for vision acuity training on adolescent myopia.

Methods

This is a prospective, randomized, multicenter clinical trial. One thousand one hundred forty primary and secondary school students aged 8–18 years old from 27 schools will be recruited and randomly divided into an experimental and a control group at a ratio of 2:1 in two phases, with a training period of 30 days in each phase and a follow-up period of 3 months. No interventions will be conducted during the follow-up period, nor will other interventions employed. Inclusion criteria will meet the diagnostic criteria for simple myopia and −6.00D ≤ spherical lenses ≤ −0.50D and cylindrical lenses ≤1.50D. The primary observation index will be to compare the statistical differences in distant visual acuity between the two groups; the secondary observation indexes will be ocular symptoms (mainly including eye fatigue, dryness, pain, double vision, neck pain, thought disorders, and lags in response), diopter, and astigmatism.

Discussion

The purpose of this two-phase trial is to compare the clinical effectiveness of focused vision-guided meditation with Chinese eye exercises that are also non-pharmacological, non-invasive interventions for myopia, and to maximize the benefit to the subjects. The results will indicate whether the training based on focused vision-guided meditation has the ability to improve distant visual acuity, relieve ocular symptoms, and ameliorate diopter. In addition, this trial will provide clinical efficacy of the training, which is expected to provide meaningful data for vision rehabilitation. At the same time, the vision acuity training method, which is permeated with the concept of Traditional Chinese Medicine (TCM) rehabilitation and health maintenance, will be applied to achieve the goal of preventing or alleviating myopic development and reducing myopia rate.

Trial registration

Chinese Clinical Trial Registry ChiCTR2000038642. Registered on 26 September 2020
Fasting before living-kidney donation: effect on donor well-being and postoperative recovery: study protocol of a multicenter randomized controlled trial
Authors: Oudmaijer C. A. J. , Minnee R. C. , Pol R. A. , van den Boogaard W. M. C. , Komninos D. S. J. , van de Wetering J. , van Heugten M. H. , Hoorn E. J. , Sanders J. S. F. , Hoeijmakers J. H. J. , Vermeij W. P. , IJzermans J. N. M.
Published: January 2022

Background

One of the main effectors on the quality of life of living-kidney donors is postoperative fatigue. Caloric restriction (CR) and short-term fasting (STF) are associated with improved fitness and increased resistance to acute stress. CR/STF increases the expression of cytoprotective genes, increases immunomodulation via increased anti-inflammatory cytokine production, and decreases the expression of pro-inflammatory markers. As such, nutritional preconditioning by CR or STF represents a non-invasive and cost-effective method that could mitigate the effects of acute surgery-induced stress and postoperative fatigue. To investigate whether preoperative STF contributes to a reduction in fatigue after living-kidney donation, a randomized clinical trial is indicated.

Methods

We aim to determine whether 2.5 days of fasting reduces postoperative fatigue score in subjects undergoing living-kidney donation. In this randomized study, the intervention group will follow a preoperative fasting regime for 2.5 days with a low-dose laxative, while the control group will receive standard care. The main study endpoint is postoperative fatigue, 4 weeks after living-kidney donation. Secondary endpoints include the effect of preoperative fasting on postoperative hospital admission time, the feasibility of STF, and the postoperative recovery of donor and recipient kidney function. This study will provide us with knowledge of the feasibility of STF and confirm its effect on postoperative recovery.

Discussion

Our study will provide clinically relevant information on the merits of caloric restriction for living-kidney donors and recipients. We expect to reduce the postoperative fatigue in living-kidney donors and improve the postoperative recovery of living-kidney recipients. It will provide evidence on the clinical merits and potential caveats of preoperative dietary interventions.

Trial registration

Netherlands Trial Register NL9262. EudraCT 2020-005445-16. MEC Erasmus MC MEC-2020-0778. CCMO NL74623.078.21
Variation in water contact behaviour and risk of Schistosoma mansoni (re)infection among Ugandan school-aged children in an area with persistent high endemicity
Authors: Trienekens Suzan C. M. , Faust Christina L. , Besigye Fred , Pickering Lucy , Tukahebwa Edridah M. , Seeley Janet , Lamberton Poppy H. L.
Published: January 2022

Background

Annual mass drug administration with praziquantel has reduced schistosomiasis transmission in some highly endemic areas, but areas with persistent high endemicity have been identified across sub-Saharan Africa, including Uganda. In these areas many children are rapidly reinfected post treatment, while some children remain uninfected or have low-intensity infections. The aim of this mixed-methods study was to better understand variation in water contact locations, behaviours and infection risk in school-aged children within an area with persistent high endemicity to inform additional control efforts.

Methods

Data were collected in Bugoto, Mayuge District, Uganda. Two risk groups were identified from a longitudinal cohort, and eight children with no/low-intensity infections and eight children with reinfections were recruited. Individual structured day-long observations with a focus on water contact were conducted over two periods in 2018. In all identified water contact sites, four snail surveys were conducted quarterly over 1 year. All observed Biomphalaria snails were collected, counted and monitored in the laboratory for Schistosoma mansoni cercarial shedding for 3 weeks.

Results

Children came into contact with water for a range of purposes, either directly at the water sources or by coming into contact with water collected previously. Although some water contact practices were similar between the risk groups, only children with reinfection were observed fetching water for commercial purposes and swimming in water sources; this latter group of children also came into contact with water at a larger variety and number of sites compared to children with no/low-intensity infection. Households with children with no/low-intensity infections collected rainwater more often. Water contact was observed at 10 sites throughout the study, and a total of 9457 Biomphalaria snails were collected from these sites over four sampling periods. Four lake sites had a significantly higher Biomphalaria choanomphala abundance, and reinfected children came into contact with water at these sites more often than children with no/low-intensity infections. While only six snails shed cercariae, four were from sites only contacted by reinfected children.

Conclusions

Children with reinfection have more high-risk water contact behaviours and accessed water sites with higher B. choanomphala abundance, demonstrating that specific water contact behaviours interact with environmental features to explain variation in risk within areas with persistent high endemicity. Targeted behaviour change, vector control and safe water supplies could reduce reinfection in school-aged children in these settings.

Graphical Abstract
Regional heterogeneity and unexpectedly high abundance of Cooperia punctata in beef cattle at a northern latitude revealed by ITS-2 rDNA nemabiome metabarcoding
Authors: De Seram Eranga Lakshitha , Redman Elizabeth Mary , Wills Felicity Kaye , de Queiroz Camila , Campbell John Ross , Waldner Cheryl Lynne , Parker Sarah Elizabeth , Avramenko Russell William , Gilleard John Stuart , Uehlinger Fabienne Dominique
Published: January 2022

Background

The species composition of cattle gastrointestinal nematode (GIN) communities can vary greatly between regions. Despite this, there is remarkably little large-scale surveillance data for cattle GIN species which is due, at least in part, to a lack of scalable diagnostic tools. This lack of regional GIN species-level data represents a major knowledge gap for evidence-based parasite management and assessing the status and impact of factors such as climate change and anthelmintic drug resistance.

Methods

This paper presents a large-scale survey of GIN in beef herds across western Canada using ITS-2 rDNA nemabiome metabarcoding. Individual fecal samples were collected from 6 to 20 randomly selected heifers (n = 1665) from each of 85 herds between September 2016 and February 2017 and 10–25 first season calves (n = 824) from each of 42 herds between November 2016 and February 2017.

Results

Gastrointestinal nematode communities in heifers and calves were similar in Alberta and Saskatchewan, with Ostertagia ostertagi and Cooperia oncophora being the predominant GIN species in all herds consistent with previous studies. However, in Manitoba, Cooperia punctata was the predominant species overall and the most abundant GIN species in calves from 4/8 beef herds.

Conclusions

This study revealed a marked regional heterogeneity of GIN species in grazing beef herds in western Canada. The predominance of C. punctata in Manitoba is unexpected, as although this parasite is often the predominant cattle GIN species in more southerly latitudes, it is generally only a minor component of cattle GIN communities in northern temperate regions. We hypothesize that the unexpected predominance of C. punctata at such a northerly latitude represents a range expansion, likely associated with changes in climate, anthelmintic use, management, and/or animal movement. Whatever the cause, these results are of practical concern since C. punctata is more pathogenic than C. oncophora, the Cooperia species that typically predominates in cooler temperate regions. Finally, this study illustrates the value of ITS-2 rDNA nemabiome metabarcoding as a surveillance tool for ruminant GIN parasites.

Graphical Abstract
Chromatin alterations during the epididymal maturation of mouse sperm refine the paternally inherited epigenome
Authors: Bedi Yudhishtar S. , Roach Alexis N. , Thomas Kara N. , Mehta Nicole A. , Golding Michael C.
Published: January 2022

Background

Paternal lifestyle choices and male exposure history have a critical influence on the health and fitness of the next generation. Accordingly, defining the processes of germline programming is essential to resolving how the epigenetic memory of paternal experiences transmits to their offspring. Established dogma holds that all facets of chromatin organization and histone posttranslational modification are complete before sperm exits the testes. However, recent clinical and animal studies suggest that patterns of DNA methylation change during epididymal maturation. In this study, we used complementary proteomic and deep-sequencing approaches to test the hypothesis that sperm posttranslational histone modifications change during epididymal transit.

Results

Using proteomic analysis to contrast immature spermatozoa and mature sperm isolated from the mouse epididymis, we find progressive changes in multiple histone posttranslational modifications, including H3K4me1, H3K27ac, H3K79me2, H3K64ac, H3K122ac, H4K16ac, H3K9me2, and H4K20me3. Interestingly, some of these changes only occurred on histone variant H3.3, and most involve chromatin modifications associated with gene enhancer activity. In contrast, the bivalent chromatin modifications, H3K4me3, and H3K27me3 remained constant. Using chromatin immunoprecipitation coupled with deep sequencing, we find that changes in histone h3, lysine 27 acetylation (H3K27ac) involve sharpening broad diffuse regions into narrow peaks centered on the promoter regions of genes driving embryonic development. Significantly, many of these regions overlap with broad domains of H3K4me3 in oocytes and ATAC-seq signatures of open chromatin identified in MII oocytes and sperm. In contrast, histone h3, lysine 9 dimethylation (H3K9me2) becomes enriched within the promoters of genes driving meiosis and in the distal enhancer regions of tissue-specific genes sequestered at the nuclear lamina. Maturing sperm contain the histone deacetylase enzymes HDAC1 and HDAC3, suggesting the NuRD complex may drive some of these changes. Finally, using Western blotting, we detected changes in chromatin modifications between caput and caudal sperm isolated from rams (Ovis aries), inferring changes in histone modifications are a shared feature of mammalian epididymal maturation.

Conclusions

These data extend our understanding of germline programming and reveal that, in addition to trafficking noncoding RNAs, changes in histone posttranslational modifications are a core feature of epididymal maturation.
Hepatocellular carcinoma patients with high circulating cytotoxic T cells and intra-tumoral immune signature benefit from pembrolizumab: results from a single-arm phase 2 trial
Authors: Hong Jung Yong , Cho Hee Jin , Sa Jason K. , Liu Xiaoqiao , Ha Sang Yun , Lee Taehyang , Kim Hajung , Kang Wonseok , Sinn Dong Hyun , Gwak Geum-Youn , Choi Moon Seok , Lee Joon Hyeok , Koh Kwang Cheol , Paik Seung Woon , Park Hee Chul , Kang Tae Wook , Rhim Hyunchul , Lee Su Jin , Cristescu Razvan , Lee Jeeyun , Paik Yong Han , Lim Ho Yeong
Published: January 2022

Background

A limited number of studies have characterized genomic properties of hepatocellular carcinoma (HCC) patients in response to anti-PD-1 immunotherapy.

Methods

Herein, we performed comprehensive molecular characterization of immediate (D-42 to D-1) pre-treatment tumor biopsy specimens from 60 patients with sorafenib-failed HCC in a single-arm prospective phase II trial of pembrolizumab. Objective response rate was the primary efficacy endpoint. We used whole-exome sequencing, RNA sequencing, and correlative analysis. In addition, we performed single-cell RNA sequencing using peripheral blood mononuclear cells.

Results

The overall response rate of pembrolizumab in sorafenib-failed HCC patients was 10% ([6/60]95% CI, 2.4–17.6). In a univariate analysis using clinicopathological features, female gender, PD-L1 positivity, and low neutrophil-to-lymphocyte ratio (NLR) were identified as contributing factors to pembrolizumab response. Somatic mutations in CTNNB1 and genomic amplifications in MET were found only in non-responders. Transcriptional profiles through RNA sequencing identified that pembrolizumab responders demonstrated T cell receptor (TCR) signaling activation with expressions of MHC genes, indicating increased levels of T cell cytotoxicity. In single-cell sequencing from 10 pre- and post-treatment peripheral blood mononuclear cells (PBMCs), patients who achieved a partial response or stable disease exhibited immunological shifts toward cytotoxic CD8+ T cells. Conversely, patients with progressive disease showed an increased number of both CD14+ and CD16+ monocytes and activation of neutrophil-associated pathways.

Conclusions

Taken together, HCC patients with infiltration of cytotoxic T cells, along with increased active circulating CD8+ T cells during pembrolizumab treatment and down-regulation of neutrophil-associated markers, significantly benefited from pembrolizumab treatment.

Trial registration

NCT#03163992(first posted: May 23, 2017)
Impact of azithromycin mass drug administration on the antibiotic-resistant gut microbiome in children: a randomized, controlled trial
Authors: Pickering Harry , Hart John D. , Burr Sarah , Stabler Richard , Maleta Ken , Kalua Khumbo , Bailey Robin L. , Holland Martin J.
Published: January 2022

Background

Mass drug administration (MDA) with azithromycin is the primary strategy for global trachoma control efforts. Numerous studies have reported secondary effects of MDA with azithromycin, including reductions in childhood mortality, diarrhoeal disease and malaria. Most recently, the MORDOR clinical trial demonstrated that MDA led to an overall reduction in all-cause childhood mortality in targeted communities. There is however concern about the potential of increased antimicrobial resistance in treated communities. This study evaluated the impact of azithromycin MDA on the prevalence of gastrointestinal carriage of macrolide-resistant bacteria in communities within the MORDOR Malawi study, additionally profiling changes in the gut microbiome after treatment. For faecal metagenomics, 60 children were sampled prior to treatment and 122 children after four rounds of MDA, half receiving azithromycin and half placebo.

Results

The proportion of bacteria carrying macrolide resistance increased after azithromycin treatment. Diversity and global community structure of the gut was minimally impacted by treatment, however abundance of several species was altered by treatment. Notably, the putative human enteropathogenEscherichia albertiiwas more abundant after treatment.

Conclusions

MDA with azithromycin increased carriage of macrolide-resistant bacteria, but had limited impact on clinically relevant bacteria. However, increased abundance of enteropathogenicEscherichiaspecies after treatment requires further, higher resolution investigation. Future studies should focus on the number of treatments and administration schedule to ensure clinical benefits continue to outweigh costs in antimicrobial resistance carriage.

Trial registrationClinicalTrial.gov, NCT02047981. Registered January 29th 2014,https://clinicaltrials.gov/ct2/show/NCT02047981
An inertial iterative algorithm for generalized equilibrium problems and Bregman relatively nonexpansive mappings in Banach spaces
Authors: Alansari Monairah , Farid Mohammad , Ali Rehan
Published: January 2022

The aim of this paper is to introduce and study an inertial hybrid iterative method for solving generalized equilibrium problems involving Bregman relatively nonexpansive mappings in Banach spaces. We study the strong convergence for the proposed algorithm. Finally, we list some consequences and computational example to emphasize the efficiency and relevancy of main result.
The use of Big Data Analytics in healthcare
Authors: Batko Kornelia , Ślęzak Andrzej
Published: January 2022

The introduction of Big Data Analytics (BDA) in healthcare will allow to use new technologies both in treatment of patients and health management. The paper aims at analyzing the possibilities of using Big Data Analytics in healthcare. The research is based on a critical analysis of the literature, as well as the presentation of selected results of direct research on the use of Big Data Analytics in medical facilities. The direct research was carried out based on research questionnaire and conducted on a sample of 217 medical facilities in Poland. Literature studies have shown that the use of Big Data Analytics can bring many benefits to medical facilities, while direct research has shown that medical facilities in Poland are moving towards data-based healthcare because they use structured and unstructured data, reach for analytics in the administrative, business and clinical area. The research positively confirmed that medical facilities are working on both structural data and unstructured data. The following kinds and sources of data can be distinguished: from databases, transaction data, unstructured content of emails and documents, data from devices and sensors. However, the use of data from social media is lower as in their activity they reach for analytics, not only in the administrative and business but also in the clinical area. It clearly shows that the decisions made in medical facilities are highly data-driven. The results of the study confirm what has been analyzed in the literature that medical facilities are moving towards data-based healthcare, together with its benefits.
Bone density may affect primary stability of anterior cruciate ligament reconstruction when organic core bone plug fixation technique used
Authors: Dehestani Pouya , Farahmand Farzam , Borjali Amirhossein , Bashti Kaveh , Chizari Mahmoud
Published: January 2022

Purpose

Core Bone Plug Fixation (CBPF) technique is an implant-less methodology for ACL reconstruction. This study investigates the effect of bone density on CBPF stability to identify the bone quality that is likely to benefit from this technique.

Methods

Artificial blocks with 160 (Group 1), 240 (Group 2), and 320 (Group 3) kg/m3 densities were used to simulate human bone with diverse qualities. These groups are representative of the elderly, middle age and young people, respectively. A tunnel was made in each test sample using a cannulated drill bit which enabled harvesting the core bone plug intact. Fresh animal tendon grafts were prepared and passed through the tunnel, so the core bone was pushed in to secure the tendon. The fixation stability was tested by applying a cyclic load following by a pullout load until the failure occurred. The selected group was compared with interference screw fixation technique as a gold standard method in ACL reconstruction.

Results

The Group 2 stiffness and yield strength were significantly larger than Group 1. The graft slippage of Group 1 was significantly less than Group 3. The ultimate strengths were 310 N and 363 N, in Groups 2 and 3, significantly larger than that of Group 1. The ultimate strength in fixation by interference screw was 693.18 N, significantly larger than the bone plug method.

Conclusions

The stability of CBPF was greatly affected by bone density. This technique is more suitable for young and middle-aged people. With further improvements, the CBPF might be an alternative ACL reconstruction technique for patients with good bone quality.

Clinical relevance

The CBPF technique offers an implant-less organic ACL reconstruction technique with numerous advantages and likely would speed up the healing process by using the patient’s own bones and tissues rather than any non-biologic fixations.
Radiomics nomogram for the preoperative prediction of lymph node metastasis in pancreatic ductal adenocarcinoma
Authors: Bian Yun , Guo Shiwei , Jiang Hui , Gao Suizhi , Shao Chengwei , Cao Kai , Fang Xu , Li Jing , Wang Li , Ma Chao , Zheng Jianming , Jin Gang , Lu Jianping
Published: January 2022

Purpose

To develop and validate a radiomics nomogram for the preoperative prediction of lymph node (LN) metastasis in pancreatic ductal adenocarcinoma (PDAC).

Materials and methods

In this retrospective study, 225 patients with surgically resected, pathologically confirmed PDAC underwent multislice computed tomography (MSCT) between January 2014 and January 2017. Radiomics features were extracted from arterial CT scans. The least absolute shrinkage and selection operator method was used to select the features. Multivariable logistic regression analysis was used to develop the predictive model, and a radiomics nomogram was built and internally validated in 45 consecutive patients with PDAC between February 2017 and December 2017. The performance of the nomogram was assessed in the training and validation cohort. Finally, the clinical usefulness of the nomogram was estimated using decision curve analysis (DCA).

Results

The radiomics signature, which consisted of 13 selected features of the arterial phase, was significantly associated with LN status (p < 0.05) in both the training and validation cohorts. The multivariable logistic regression model included the radiomics signature and CT-reported LN status. The individualized prediction nomogram showed good discrimination in the training cohort [area under the curve (AUC), 0.75; 95% confidence interval (CI), 0.68–0.82] and in the validation cohort (AUC, 0.81; 95% CI, 0.69–0.94) and good calibration. DCA demonstrated that the radiomics nomogram was clinically useful.

Conclusions

The presented radiomics nomogram that incorporates the radiomics signature and CT-reported LN status is a noninvasive, preoperative prediction tool with favorable predictive accuracy for LN metastasis in patients with PDAC.
Retrospective Analysis of Training Intensity Distribution Based on Race Pace Versus Physiological Benchmarks in Highly Trained Sprint Kayakers
Authors: Matzka Manuel , Leppich Robert , Sperlich Billy , Zinner Christoph
Published: January 2022

Background

Research results on the training intensity distribution (TID) in endurance athletes are equivocal. This non-uniformity appears to be partially founded in the different quantification methods that are implemented. So far, TID research has solely focused on sports involving the lower-body muscles as prime movers (e.g. running). Sprint kayaking imposes high demands on the upper-body endurance capacity of the athlete. As there are structural and physiological differences between upper- and lower-body musculature, TID in kayaking should be different to lower-body dominant sports. Therefore, we aimed to compare the training intensity distribution during an 8-wk macrocycle in a group of highly trained sprint kayakers employing three different methods of training intensity quantification.

Methods

Heart rate (HR) and velocity during on-water training of nine highly trained German sprint kayakers were recorded during the final 8 weeks of a competition period leading to the national championships. The fractional analysis of TID was based on three zones (Z) derived from either HR (TIDBla-HR) or velocity (TIDBla-V) based on blood lactate (Bla) concentrations (Z1 ≤ 2.5 mmol L−1Bla, Z2 = 2.5–4.0 mmol L−1Bla, Z3 ≥ 4.0 mmol L−1Bla) of an incremental test or the 1000-m race pace (TIDRace): Z1 ≤ 85% of race pace, Z2 = 86–95% and Z3 ≥ 95%.

Results

TIDBla-V(Z1: 68%, Z2: 14%, Z3: 18%) differed from TIDBla-HR(Z1: 91%, Z2: 6%, Z3: 3%) in each zone (allp < 0.01). TIDRace(Z1: 73%, Z2: 20%, Z3: 7%) differed to Z3 in TIDBla-V(p < 0.01) and all three TIDBla-HRzones (allp < 0.01). Individual analysis revealed ranges of Z1, Z2, Z3 fractions for TIDBla-HRof 85–98%, 2–11% and 0.1–6%. For TIDBla-V, the individual ranges were 41–82% (Z1), 6–30% (Z2) and 8–30% (Z3) and for TIDRace64–81% (Z1), 14–29% (Z2) and 4–10% (Z3).

Conclusion

The results show that the method of training intensity quantification substantially affects the fraction of TID in well-trained sprint kayakers. TIDRacedetermination shows low interindividual variation compared to the physiologically based TIDBla-HRand TIDBla-V. Depending on the aim of the analysis TIDRace, TIDBla-HRand TIDBla-Vhave advantages as well as drawbacks and may be implemented in conjunction to maximize adaptation.
Conjunctival lymphangiectasia and retinal angiopathy in hereditary transthyretin amyloidosis
Authors: Patil Nikhil S. , Iqbal Munir M. , Bursztyn Lulu L. C. D.
Published: January 2022

Background

Hereditary transthyretin amyloidosis (ATTR amyloidosis) is a rare condition where a mutation in the transthyretin gene leads to systemic deposition of amyloid. The manifestations and prognosis of ATTR amyloidosis depends on the specific ATTR mutation, with over 100 mutations reported in the literature. The manifestations of many rare forms of ATTR amyloidosis have not been well described, particularly the late-onset ophthalmic findings.

Case presentation

We present the case of a 43-year-old Caucasian male with a diagnosis of ATTRD18E amyloidosis confirmed by fat pad biopsy. He had diffuse systemic involvement, including cardiovascular, pulmonary, and gastrointestinal symptoms. He also had significant ocular involvement including vitreous opacities, retinal angiopathy, and conjunctival lymphangiectasia. These ocular findings modestly progressed at 2-year follow-up.

Discussion

The ATTRD18E mutation is a rare variant, with few described cases. To our knowledge, this is the first documented case of ATTRD18E amyloidosis with significant ocular involvement. These ocular findings may serve as a relevant biomarker for severe disease prognosis in ATTRD18E amyloidosis. With improving treatments addressing the systemic symptoms of ATTR amyloidosis, a better understanding of the late-onset ocular symptoms is becoming increasingly relevant.
What do they TEL(L)? A systematic analysis of master programs in technology-enhanced learning
Authors: Fominykh Mikhail , Weidlich Joshua , Kalz Marco , Hybertsen Ingunn Dahler
Published: January 2022

This article contributes to the debate on the growing number of interdisciplinary study programs in learning and technology, and aims to understand the diversity of programs as well as curricula structure in an international landscape. Scientific fields share their knowledge and recruit young researchers by offering discipline-specific study programs. Thus, study programs are a reflection of the fields they represent. As technology-enhanced learning is considered to be particularly interdisciplinary and heterogenous, it is important to better understand the landscape of study programs that represents the field. This article presents an analysis of master programs in technology-enhanced learning. A systematic review and analysis of master programs offered in English has been conducted and further used as input for hierarchical cluster analysis. The study identified general characteristics, curricula structure, and organization of topics of these programs. Hierarchical cluster analysis and qualitative content analysis helped us to identify the major types of curricular structures and typical topics covered by the courses. Results show that most study programs rely on interdisciplinary subjects in technology-enhanced learning with a considerable number of subjects from education, learning and psychology. Subjects related to technology, information and computer science appear in such programs less frequently.
What do we know about evidence-informed priority setting processes to set population-level health-research agendas: an overview of reviews
Authors: Tan Audrey , Nagraj Sumanth Kumbagere , Nasser Mona , Sharma Tarang , Kuchenmüller Tanja
Published: January 2022

Background

This overview aimed to synthesize existing systematic reviews to produce a draft framework of evidence-informed health priority setting that supports countries in identifying appropriate steps and methods when developing and implementing national research agendas.

Main body

We searched Ovid MEDLINE® and the WHO Institutional Repository for Information Sharing from 2010 to 2020 for critical or systematic reviews that evaluated research priority setting exercises. We adapted the AMSTAR checklist to assess the quality of included reviews and used adapted frameworks for data extraction and analysis. The search resulted in 2395 titles, of which 31 were included. Populations included in the reviews typically involved patients, families and carers, researchers, clinicians, policymakers and research funders. The topics covered in the reviews varied from specific diseases or conditions, approaches for healthcare practice or research priority setting methods itself. All the included systematic reviews were of low or critically low quality. The studies were thematically grouped based on their main focus: identifying and engaging with stakeholders; methods; context; and health area.

Conclusion

Our overview of reviews has reconfirmed aspects of existing frameworks, but has also identified new concepts for countries to consider while developing their national research agendas. We propose a preliminary framework for consideration that highlights four key phases: (1) preparatory, (2) priority setting, (3) follow-up phase and (4) sustainability phase, which have thirteen sub-domains to consider.
Multifunctional CuO nanoparticles with enhanced photocatalytic dye degradation and antibacterial activity
Authors: Dulta Kanika , Koşarsoy Ağçeli Gözde , Chauhan Parveen , Jasrotia Rohit , Chauhan P. K. , Ighalo Joshua O.
Published: January 2022

Rhizome extract ofBergenia ciliatawas used as a bio-functional reducing material for the green synthesis of copper oxide nanoparticles (CuO NPs). CuO NPs were characterized using ultraviolet–visible spectroscopy, Fourier transforms infrared spectroscopy, X-ray diffraction (XRD), dynamic light scattering, scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDX). XRD analysis revealed the monoclinic phase of synthesized CuO NPs with an average particle size of 20 nm. Spherical shaped nanoscale CuO particles were observed by EDX and SEM confirming the Cu and O presence in the synthesized NPs. CuO NPs showed antibacterial effects againstBacillus subtilis, Staphylococcus aureus, Escherichia coli,Salmonella typhi. The antioxidant effect was measured and IC50values for 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl and Ferric reducing antioxidant power assays were found to be 91.2, 72.4 and 109 μg mL− 1respectively. Under sunlight, the CuO NPs reported extraordinary photocatalytic activity against Methylene Blue and Methyl Red degradation with efficiencies of 92–85%. CuO NPs have excellent potential application for the photocatalytic degradation of organic pollutants and in the development of antibacterial materials. This study offers new insights in the field of inexpensive and green synthesis-based antimicrobial effective CuO photocatalysts fromB. ciliatato remove harmful dyes from industrial-based waters with high degradation efficiency, which is environmentally friendly.
The role of MR venography with time-resolved imaging in diagnosis of pelvic congestion syndrome
Authors: Attia Noha M. , Sayed Mohamed AbuDeif , Galal Mohamed Hossam ElDin , AbdelAleem Mahmoud Ahmed
Published: January 2022

Background

Pelvic congestion syndrome (PCS) represents a diagnostic challenge due to its variable clinical presentation, complex anatomy, and pathophysiology. Accurate delineation of the venous anatomy, detection of venous reflux or obstruction, its extent will enable interventional radiologists to successfully treat such patients and to avoid recurrence. Magnetic resonance imaging (MRI) allows a noninvasive examination of the anatomy and flow inside the pelvic veins in addition to its excellent soft-tissue contrast allowing evaluation of the pelvic organs. Our study is aiming to investigate the role and accuracy of MR venography with time-resolved imaging (TR-MRV) as a diagnostic tool for pretreatment planning of PCS.

Results

Our study included 25 female patients with mean age 48 ± 12.34, who were referred to the radiology department in the period from April/2019 to April/2020 with clinical and ultrasound features suggesting PCS. TR-MRV was performed and interpreted in a blind fashion evaluating the vascular anatomy, venous dilatation, and reflux. The results were compared to conventional venography as a reference. The sensitivity, specificity, and accuracy of TR-MRV in the detection of ovarian vein reflux were 87%, 80%, and 84%, respectively, versus 75%, 53%, and 72% in internal iliac vein reflux and 92%, 69%, and 64% for pelvic venous plexus reflux. Demonstration of the venous anatomy was excellent in 68% of the patients and was sufficient in 32%. Ovarian vein dilatation was detected in 16 patients by venography and in 10 patients by TR-MRV. The weighted k-values (Cohen's Kappa coefficient statistics) indicated excellent agreement between the two observers for identifying all the refluxing veins by TRI in each patient (k = 0.78).

Conclusion

MRI with TR imaging has shown high diagnostic accuracy when compared to conventional venography in evaluating pelvic congestion syndrome before endovascular treatment and thus facilitating treatment planning.
Factorial design approach to fabricate and optimize floating tablets based on combination of natural polymer and rice bran wax
Authors: Sabale Vidya P. , Gadge Gunjan G.
Published: January 2022

Background

The aim of the present research work was to fabricate a novel gastroretentive drug delivery system in the form of tablets using a combination of natural polymer and rice bran wax with an intention to control drug delivery and to enhance the gastric residence time of the model drug Famotidine in the gastrointestinal tract.

Results

The results of the preliminary trial batches prepared by using the hot melt granulation technique resulting in six different formulations showed good physicochemical characteristics and tablets conformed to the Pharmacopoeial specifications. Gastroretentive tablets containing natural polymer showed prolonged drug release comparable to Methocel. The optimized formulation (C3) using 32 factorial design showed FLT 27 ± 2.47 s, SI 92.68 ± 1.36% and % CDR 98.89 ± 0.39% at 12 h. The stability studies indicated the stability of the formulation during storage.

Conclusions

It was concluded that the release profile fitted best to zero-order equation with non-Fickian diffusion mechanism of drug release which demonstrates swelling-controlled drug release mechanism. Thus, the formulated tablets have the potential for improved release and gastroretentive properties.

Graphical Abstract
Synthesis and in vitro antimicrobial activity of new steroidal hydrazone derivatives
Authors: Mistry Shailesh , Singh Akhilesh Kumar
Published: January 2022

Background

For many years, various drugs have been used for the treatment of infectious diseases but some bacterial microorganisms have induced resistance to several drugs. In a search of new antimicrobial agents, a series of new steroidal hydrazones were designed and synthesized.

Result

The structures of the compounds were established based on the spectral data. The in vitro antimicrobial activity of some newly synthesized compounds against bacteria and fungi was studied.

Conclusion

New compounds showed better or similar antimicrobial activity. Designing more efficient steroidal hydrazones from ketosteroid based on the current study may successfully lead to the development of antimicrobial agent.

Graphical abstract
Esophageal motility abnormalities in Egyptian patients using high resolution esophageal manometry: a descriptive study
Authors: Naguib Gina Gamal , Hassan Mohamed , Elshafie Ahmed I. , Naguib M. G.
Published: January 2022

Background and aim

For many years, esophageal manometry has been used for assessment of upper gastro-intestinal (GI) symptoms. Chicago classification is the key for diagnosis and managing motility disorders as it is considered as a standardized approach for categorization of esophageal abnormalities. The aim of this study is to analyze types of esophageal motility findings in Egyptian cases who were suffering from upper GI complaints. Methods: This descriptive study included 378 subjects who were suffering from upper GI complaints as dysphagia, vomiting, chest pain and regurgitation in the period between 10/2015–7/2020. Esophageal HRM study was performed for all patients (MMS Laborie device). The catheter was positioned and confirmed passing across the EGJ (esophago-gastric junction) using landmarks. Swallows and resting status were recorded. Anatomical landmarks were placed.

Results

Most of the patients were complaining of upper GI symptoms. Males were 49.2% of cases. Mean age was 41.3. Dysphagia was the prominent symptom while chest pain was the least symptom. Many manometry findings were observed including ineffective motility, achalasia, absent contractility, EGJ outflow obstruction, jackhammer esophagus and normal findings. Type II achalasia was the dominant type in achalasia patients while Type III was the least. LES was normotensive in most of the cases. Hiatus hernia (HH) was detected in 40.2% of the cases.

Conclusion

This is considered the first Egyptian descriptive study to determine the prevalence of esophageal motility abnormalities in Egyptian patients complaining of upper GI symptoms. HRM is very important for patients complaining of upper GI symptoms.
Vitamin D level in Egyptian children with otitis media with effusion
Authors: Ahmed Ahmed Nabil Abdelhamid , Mohamed Ahmed Adly , Elbegermy Marwa Mohamed , Abdelghafar Mohamed Ali , Teaima Ahmed Abdelmoneim
Published: January 2022

Background

This prospective case-control study was conducted from June 2018 to October 2019 in a tertiary referral hospital to investigate the correlation between otitis media with effusion (OME) and serum vitamin D level in children. The study population included 50 children with adenotonsillar hypertrophy and OME (group A) who underwent adenotonsillectomy with tympanostomy tubes insertion compared to 50 children free from any medical or surgical disease (control group). Serum 25-hydroxy vitamin D was measured using electrochemiluminescence technique for both groups.

Results

The mean age in group A was 53.4 ± 9.2 months whereas in group B (control group) it was 65.0 ± 13.2 months (P <0.001). In group A, there were 29 (58 %) male children and 21 (42%) female children while in group B, there were 27 (54%) male children and 23 (46%) female children (P = 0.689). The mean vitamin D level in group A was 16.0 ± 6.1 ng/mL with a minimum of 7 ng/mL and a maximum of 32.10 ng/mL. In group B, the mean vitamin D level was 15.7 ± 5.3ng/mL with a minimum of 7.38 ng/mL and a maximum of 27.90 ng/mL. The statistical analysis showed that there was no significant difference in the level of vitamin D level between both groups (P = 0.770).

Conclusion

In this study, the mean level of serum vitamin D was low in children suffering from OME with adenotonsillar hypertrophy and in children without any medical or surgical disease; however, there was no statistically significant difference in the mean serum level of vitamin D between both groups. Therefore, a further study on a larger sample is needed.
An affine generalized optimal scheme with improved free-surface expression using adaptive strategy for frequency-domain elastic wave equation
Authors: Dong Shu-Li , Chen Jing-Bo
Published: January 2022

Effective frequency-domain numerical schemes are central for forward modeling and inversion of the elastic wave equation. The rotated optimal nine-point scheme is a highly utilized finite-difference (FD) numerical scheme. This scheme makes a weighted average of the derivative terms of the elastic wave equations in both the original and the rotated coordinate systems. In comparison with the classical nine-point scheme, it can simulate S-waves better and has higher accuracy at nearly the same computational cost. Nevertheless, this scheme limits the rotation angle to 45°; thus, the grid sampling intervals in the x- and z-directions need to be equal. Otherwise, the grid points will not lie on the axes, which dramatically complicates the scheme. Affine coordinate systems do not constrain axes to be perpendicular to each other, providing enhanced flexibility. Based on the affine coordinate transformations, we developed a new affine generalized optimal nine-point scheme. At the free surface, we applied the improved free-surface expression with an adaptive parameter-modified strategy. The new optimal scheme had no restriction that the rotation angle must be 45°. Dispersion analysis shows that our scheme can effectively reduce the required number of grid points per shear wavelength for both equal and unequal sampling intervals compared to the classical nine-point scheme. Moreover, this reduction improves with the increase of Poisson’s ratio. Three numerical examples demonstrate that our scheme can provide more accurate results than the classical nine-point scheme in terms of both the internal and free-surface grid points.
Impact of Health Perception and Knowledge on Genetic Testing Decisions Using the Health Belief Model
Authors: Elkefi Safa , Choudhury Avishek , Strachna Olga , Asan Onur
Published: January 2022

PURPOSE

Early detection of cancer risk is essential as it is associated with a higher chance of survival, more successful treatment, and improved quality of life. Genetic testing helps at-risk patients estimate the likelihood of developing cancer in a lifetime. This study aims to indentify the factors (perceived susceptibility, severity, benefits, and self-efficacy) that impact one's decision to take the genetic test.

METHODS

We examined the impacts of different factors of the health belief model on the engagement of patients in genetic testing using data from the National Cancer Institute's 2020 cross-sectional nationally representative data published in 2021. Complete surveys were answered by 3,865 participants (weighted population size = 253,815,197). All estimates were weighted to be nationally representative of the US population using the jackknife weighting method for parameter estimation. We used multivariable logistic regression to test our hypotheses for patients who have taken the genetic test for cancer risk detection. We adjusted the multivariate model for age, education, income, race, sex, cancer history, familial cancer history, and education.

RESULTS

We tested five hypotheses using the health belief model. Respondents who had genetic testing were more likely to rely on their health care providers and genetic counselors to make their decisions. Respondents who had genetic tests also reported less reliability on other sources than doctors: for the internet and social media (odds ratio = 0.33; P < .001) and for journals and magazines (odds ratio = 0.48; P = .007).

CONCLUSION

The findings show that patients generally rely on suggestions from their health care providers and counselors in genetic testing decisions. These findings also indicate that health care providers play a critical role in helping patients decide whether to use genetic testing to detect cancer risk in the early stages.