bluuebunny/crossref_metadata_2025 huggingface dataset:
Motivational and Semantic Relations of the Individual: Psychological Aspects of Managerial Control in Professional Activity
Authors: Kamalova Oksana Maksimovna
Published: May 2024

The article considers the main psychological aspects of managerial control and their influence on the motivational-semantic relations of a person in professional activity. The relevance of the research lies in the study of the structure of managerial control from a psychological point of view, which allows to identify problematic psychological aspects of managerial control that affect the motivational and semantic relations of employees. The purpose of the study is to assess the impact of various types of managerial control on the motivational and semantic relations of employees. The methodological basis of the study is the research of domestic and foreign scientists, revealing the understanding of various types of managerial control and their impact on the motivational and semantic relations of the individual. The main conclusion of the study is that managerial control has an impact on the motivational-semantic relations of the individual in professional activity. The scientific novelty of the work lies in the study of the psychological aspects of managerial control and their impact on the motivational-semantic relations of the individual to create a favorable working atmosphere that provides maximum motivation and performance of the individual in the organization. The practical significance of the study lies in the fact that the results of the study can be useful to the heads of organizations for a better understanding of the importance of choosing and applying certain types of management control. In order to increase the efficiency of professional activities, the heads of organizations were given recommendations on improving management control in the organization.
Diversity of orchid types in the Oeluan Tourism Forest, Bijeli Village, Noemuti District, North Central Timor Regency
Authors: Dewi Ni Putu Yuni Astriani , Angket Stefanus , Ledheng Ludgardis , Sele Yunawati , Welsiliana
Published: May 2024

Indonesia as a mega biodiversity country has a variety of fauna and flora. One of the floras that is often found in Indonesia is orchids. Orchids have high economic value and are able to balance the ecosystem in their habitat. This study was aimed at determining the diversity of orchid species in the Oeluan tourism forest. The current study employed a quantitative descriptive study by using the transect and the purposive sampling method. The research was conducted in February at the Oeluan Tourism Forest. The results of this study indicated that there were four terrestrial orchid genera and three epiphytic orchid genera. The analysis of the diversity index of orchid species in the Oeluan tourism forest resulted in the diversity index of (H’) 1.0438 in the medium category. The measured environmental factors at the study site were an average soil pH of 7, an average humidity of 76.33% and an average air temperature of 27.400C. The diversity of orchid species in the Oeluan tourism forest, bijeli village, Noemuti district, North Central Timor regency, East Nusa Tenggara Province, was included in the medium category.
Carotid plaque volume measurement in ischemic stroke: A pilot study
Authors: Koshy Kiren George , M Veerendra Kumar , Ramakrishnan Subasree , Kenchaiah Raghavendra , Arvinda H. R.
Published: May 2024

Objectives:

Carotid atherosclerosis is a significant risk factor for acute ischemic stroke. Three-dimensional (3D) sonography is a new technique that can be used to analyze carotid plaque both quantitatively and qualitatively. The aim was to study carotid atherosclerosis in stroke patients and healthy controls in terms of plaque volume quantification by 3D ultrasound (US).

Materials and Methods:

An observational descriptive study was conducted in the stroke unit of a university referral hospital in South India. Patients with ischemic stroke between the ages of 20 and 70 years were studied, along with age- and sex-matched controls. Carotid sonography (2D and 3D) along with Doppler studies was done in all patients, using Philips Affiniti 50 US system. Vascular plaque quantification software was used to assess plaque volume.

Results:

Twenty-four subjects were recruited, and two were excluded from the study. Twelve were cases, and ten were controls. The mean carotid intima-media thickness (CIMT) (average CIMT in six sites in common carotid artery, three on each side) studied in patients was 0.65 ± 0.10 mm, while that for healthy subjects was 0.62 ± 0.06 mm. The mean plaque volume in patients with stroke or transient ischemic attack was 179.82 ± 310.3 mm3, and that in healthy subjects was 56.75 ± 69.6 mm3. Plaque heterogeneity, surface irregularity, and ulceration were found to be common in symptomatic carotid plaques.

Conclusion:

The 3D sonography is a non-invasive and simple feasible tool for the analysis of carotid plaque.
The Genre of Ballet in the Co-Creation of Leonard Bernstein and Jerome Robbins (based on the “Fancy Free” ballet)
Authors: Jian Yang
Published: May 2024

The article is devoted to the distinctive features of the works of the American composer Leonard Bernstein (1918–1990) in the ballet genre and the tandem of the music creator and the choreographer. A signifi cant contribution to art was made by the joint work of Leonard Bernstein and Jerome Robbins. The author of the article examines the role of music in the work of the 20th century, the stylistic features relating to this period, the history of the classical dance development in the United States of America and the importance of teamwork between two creators. The previous century was marked by the enrichment of the musical word in the ballet performances context: the orchestra becomes not only a mechanism accompanying dancers, but also a full-fl edged part of the performance. Music acquires specifi city in character and conveys the images of the heroes. In the canvas of the performance one can guess the leitmotifs related to each image, running like a red thread through the entire form of the work. “Fancy free” is a work that occupies a special place in the composer’s creative legacy. Subsequently, the musical language of “Fancy Free” can be found in Leo Bernstein’s future works – the musicals “In the City” and “West Side Story”; the author’s handwriting remained true to the style.
Основные векторы творческого поиска в исполнительской деятельности К. А. Эрдели
Authors: М.М. Подгузова
Published: May 2024

Статья посвящена исполнительской деятельности выдающейся отечественной арфистки К.А. Эрдели. Широко известно, что концертирование являлось одним из основных направлений ее творчества, которому было посвящено около семи десятков лет жизни исполнительницы. Ксения Александровна выступала на разных концертных площадках царской России и Советского Союза. География ее гастрольных поездок поражает своей широтой и охватом фактически всех городов нашей страны. В каждом из них она старалась сделать свой инструмент любимым, доступным и близким всем без исключения слушателям. В предлагаемой публикации впервые предприняты попытки анализа исполнительской деятельности К.А. Эрдели. Рассмотрены основные векторы творческого поиска в контексте активной работы арфистки по обогащению и качественному обновлению концертного репертуара. Сделан вывод о принципиальном значении для отечественного арфового искусства впервые введенной ею практики сольных арфовых концертов. Представленные в статье архивные документы — письма зарубежных коллег, программа концерта исполнительницы, списки ее неопубликованных переложений и редакций для ансамблей арф и камерных составов с участием инструмента — позволяют сделать вывод о беспрецедентном значении для отечественного арфового искусства деятельности К.А. Эрдели, сумевшей поднять исполнительство на данном инструменте на небывалую до того времени высоту.

The article is devoted to performing activities of the outstanding domestic harpist K.A. Erdely. It is widely known that concert performance was one of the main directions of her work, taking about seven decades of the performer’s life. Ksenia Alexandrovna performed at various concert venues in Tsarist Russia and the Soviet Union. The geography of her tours is striking, covering practically all cities of our country. In each of them, she tried to make her instrument loved, accessible and close to all listeners without exception. The proposed publication is the first to make an attempt to analyze the performance activities of K.A. Erdely, considering the main vectors of her creative search and active work to enrich and update the quality of her concert repertoire. The conclusion is made about fundamental importance of the practice of solo harp concerts, that she was the first to introduce, for the domestic harp art. The archival documents presented in the article — letters from foreign colleagues, the concert program of the performer, lists of her unpublished transcriptions and editions for harp ensembles and chamber compositions with the instrument — allow us to conclude that the activity of K.A. Erdely is unprecedented for the domestic harp art, as managed to raise the level of harp performance, to an unprecedented height.
Comparative analysis of approaches to assessing the market cost of business in domestic and foreign practice
Authors: Gavrilova El. N. , Hasjanova D. N.
Published: May 2024

The greatest demand in the modern world is for business valuation service. Valuation activity makes it possible to identify the important characteristics of a particular business which is necessary for making management decisions by company owners. The results of business valuation are used to determine existing perspectives: the presence of investment opportunities, ways to optimise business processes, need to expand business activities, etc. According to statistics, several decades ago, business valuation was conducted in no more than 10 % of cases. Currently, on the contrary, more than 90 % of companies are interested in regular valuation. However, the widespread demand for business valuation has made it possible to find a number of pressing problems that need to be solved in the future. One of the most serious сhallenges is the existence of problem areas in the national legal framework. The work examines the process of business valuation using basic methods and approaches, analyses the foreign experience on the research topic and also valuates a domestic enterprise in different ways. The authors concluded that it is necessary to choose the method that can be most effective in valuating a particular enterprise in particular conditions.
Historical background and basic elements of the ideal of Chinese traditional management education
Authors: Zakharov M. Yu. , Shishkova A. V.
Published: May 2024

The article is devoted to determining the specifics of the ideal of traditional management education in China in the context of the modern strategy of ­China’s digital breakthrough. Using historical-philosophical, comparative and hermeneutic methods, the authors refer to the works of thinkers of Old China and modern researchers on the topic of education and public administration, and identify the components of the ideal traditional management education. It is stated that the ideal of management education is historical in nature, continuously developing and consists of a traditional core, which needs to be preserved and protected, and a “floating” part that is always adjusted in the conditions of changing reality. The Chinese society is characterised by the constant presence among the components of the national idea that lies in the dream to form a perfect managerial education based on historical practice which solved the state task of training managers of various levels. The historical prerequisites for the formation of such an ideal are described and the development of the modern system of education of civil servant is shown. Three main elements of the traditional education system are emphasised: concentration of knowledge under control of the national caste of managers; cognitive duality teacher-pupil as the basis of the learning process; stimulation of public education in contrast to home schooling. It is concluded that effective managerial education is impossible without a socio-cultural basis, a kind of educational ideal which fulfills the regulatory function.
The stress level of future teachers depending on the direction of professional subject training
Authors: Galoy N. Yu.
Published: May 2024

The article describes the influence of university educational environment on the formation of resistance to stress in future teachers. A theoretical analysis of the phenomenon of psychological stress as a state of mental tension manifested in emotional, behavioural and somatic sensations is presented. The results of testing 529 students, who were examined in April 2022, using the PSN-25 methodology were analysed. The features of the stress level of future teachers are formulated and statistically substantiated, depending on the direction of professional subject training (foreign language, music, physical education), course of study, gender. It was found that more than half of all respondents do not experience excessive mental stress. The number of students with a low level of stress increases from the first year to the third one, regardless of the direction of professional subject training. Among future foreign language teachers, a relatively higher level of mental stress prevails. This trend is statistically significant for the first-year students of the sample. Future of physical education and music teachers are more normalised in terms of mental stress which may be due to the peculiarities of educational environment of these faculties and due to the nature of educational and professional activities. Significant gender differences in the prevalence of higher stress levels in young ladies compared to young men were revealed in a sample of these two directions of training.
Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays
Authors: Brambilla Dario , Panico Federica , Zarini Lorenzo , Mussida Alessandro , Ferretti Anna Maria , Aslan Mete , Ünlü M. Selim , Chiari Marcella
Published: May 2024

Gold nanoparticles (AuNPs) play a vital role in biotechnology, medicine, and diagnostics due to their unique optical properties. Their conjugation with antibodies, antigens, proteins, or nucleic acids enables precise targeting and enhances biosensing capabilities. Functionalized AuNPs, however, may experience reduced stability, leading to aggregation or loss of functionality, especially in complex biological environments. Additionally, they can show non-specific binding to unintended targets impairing assay specificity. Within this work, citrate-stabilized and silica coated AuNPs (GNPs and SiGNPs, respectively) have been coated using N,N-dimethylacrylamide-based copolymers to increase their stability and enable their functionalization with biomolecules. AuNPs stability after modification has been assessed by a combination of techniques including spectrophotometric characterization, nanoparticle tracking analysis, transmission electron microscopy and functional microarray tests. Two different copolymers were identified to provide a stable coating of AuNPs while enabling further modification through click chemistry reactions, due to the presence of azide groups in the polymers. Following this experimental design, AuNPs decorated with ssDNA and streptavidin were synthesized and successfully used in a biological assay. In conclusion, a functionalization scheme for AuNPs has been developed that offers ease of modification, often requiring single steps and short incubation time. The obtained functionalized AuNPs offer huge flexibility, as the functionalization protocol can be personalized to match requirements of multiple assays.
Plasma-Structured Molybdenum Oxycarbides Enabling Ultrastable Acidic Hydrogen Evolution up to 10 A cm-2
Authors: Wu Shiwen , Hwang Taesoon , Mashhadian Amirarsalan , Li Tianyi , Cho Kyeongjae , Xiong Guoping
Published: May 2024

Fabricating electrocatalysts capable of stable operation at high current densities is crucial for the industrial proton exchange membrane water electrolysis. However, current catalysts suffer from high overpotentials and rapid degradation when working in acid electrolytes at high current densities. In this paper, we report the design of vertical-standing, nanoedge-enriched molybdenum oxycarbide electrocatalysts via plasma-enhanced chemical vapor deposition (PECVD) with salts as precursors to achieve outstanding acidic electrocatalytic hydrogen evolution performance. Benefiting from their unique structural features and chemical compositions, the plasma-grown molybdenum oxycarbide catalysts exhibit a low overpotential of 415 mV at industrial-level high current densities up to 10 A cm-2 for 1,000 h, corresponding to an ultrahigh hydrogen throughput of 4,477.4 L cm-2 and substantially surpassing the performance of state-of-the-art transition metal- and even noble metal-based catalysts. This work paves the way for the development of new transition metal-based catalysts for practical industrial electrocatalytic hydrogen evolution.
Particle-particle interface corrosion of cold sprayed copper in dilute nitric acid solution: Geometry-controlled corrosion mechanism
Authors: Li Xuejie , Filice Fraser , Henderson Jeffrey , Behazin Mehran , Ramamurthy Sridhar , Barker Ivan , Skaanvik Sebastian , Moshrefi Reza , Gateman Samantha , Shoesmith David , Noël James
Published: May 2024

The mechanism of particle-particle interface (PPI) corrosion observed in cold sprayed (CS) Cu immersed in dilute HNO3 has been elucidated. PPI corrosion is initiated by the oxide inclusions present along the PPIs. The accelerated corrosion rate at PPIs results from the combined effects of confined geometry and catalytic reactions, which involve the electrochemical dissolution of Cu and reduction of NO3−. Annealing the CS Cu at 600°C coalesces the oxide inclusions, thereby breaking the interconnected oxide inclusion network. As a result, the propagation of PPI corrosion is impeded.
Factors influencing microbiological, physicochemical quality, and yield of dry-aging beef
Authors: Hanagasaki T.
Published: May 2024

The dry-aging business is widely popular across Japan. In Okinawa, most dry-aged beef is
usually produced using imported beef. Microorganisms are likely to thrive on the surface
of imported beef during transport to Japan, despite storage at chilled temperatures. To
prevent the growth of microorganisms in such cases, accurate control of aging is critical.
A fundamental understanding on a scientific level with regard to the maintenance of
hygiene is essential for developing a method for dry-aged products. In this study, changes
were analyzed in the water activity and the microorganisms on beef meat, in addition to
moisture and trim losses (aging loss), during aging under varying levels of relative
humidity and temperature, with or without airflow, and mold treatment. As the relative
humidity during the aging process was lower, the number of microorganisms was lower
but the aging loss was higher. Similarly, as the temperature during the aging process was
lower, the number of microorganisms was lower, and the aging loss remained almost
unchanged. Hence, according to the results, it is essential to lower water activity as
quickly as possible to 0.94 by keeping relative humidity below 75% as a guide and
freezing temperatures below 4°C but above −2 to −3°C for meat is strongly recommended.
In addition, direct airflow to the beef during the aging process prevented the growth of
microorganisms. In particular, it decreased Escherichia spp. but increase aging loss. As a
result, air circulation via moderate airflow is recommended during the aging process.
Besides, it became apparent that mold treatment did not prevent the growth of
microorganisms.
THE ROLE OF THEME ROUTES IN THE DEVELOPMENT OF RURAL TOURISM
Authors: Pentz Mónika
Published: May 2024

Purpose: The purpose of this study was to draw attention to the positive tourism development effects of thematic routes in rural areas, using the analysis results of good practices in Germany.
 
Theoretical Framework: The interpretation of the thematic routes and the history of their creation are presented with the help of the literature related to the topic. The examination of the issue is incomplete, so the work aims to fill a research gap.
 
Design/Methodology/Approach: In the ongoing research, in addition to the analysis of secondary sources, we performed a website analysis and visited the most important thematic routes using the methodology of personal qualitative observation. We have created a database covering more than 250 thematic routes.
 
Findings: Good practices discovered during our research confirm that the development of tourist thematic routes can be an effective tool for the protection of rural heritage and for the development of rural gastronomy, in the presence of a suitable management organization.
 
Research, Practical & Social Implications: The German practice can serve as a good example in other countries in the development and operation of effective tourist thematic routes. However, effective operation requires a responsible "host" organization, professional management, and it is necessary to take into account trends affecting the demand-side of tourism during development.
 
Originality/Value: The results show the chronological distribution of the development of the thematic routes, and contain a recommendation on the important aspects to be taken into account during development.
KEJADIAN BERAT BADAN LAHIR RENDAH BERDASARKAN RIWAYAT KEHAMILAN
Authors: Wigati Atun , Islami Islami , Astuti Dwi
Published: May 2024

Prevalensi BBLR di Indonesia berdasarkan data Riskesdas pada tahun 2013 sebesar 10,2 % dan sedikit meningkat menjadi 11,1% pada tahun 2015 selanjutnya tercatat menurun menjadi 6,2% pada tahun 2018. Sedangkan di Jawa Tengah angka kejadian BBLR mencapai 6,0%. Tujuan dari penelitian ini adalah adalah Untuk menganalisis korelasi antara kejadian bayi berat lahir rendah dengan riwayat kehamilan  dengan di  Wilayah Kerja Puskesmas Rendeng kabupaten Kudus. Metode Penelitian, penelitian observasional dengan pendekatan Case Control yaitu penelitian analitik yang menyangkut bagaimana faktor risiko dipelajari dengan menggunakan observasi secara retrospective. populasi dalam penelitian ini adalah bayi yang  ada di wilayah kerja puskesmas Rendeng sejumlah 60 Bayi baru lahir. Jumlah sampel pada penelitian ini yaitu 31 balita usia 12 bulan . Teknik sampling mennggunakan Accidental sampling. Metode Pengumpulan data meliputi  dokumentasi  dan kuesioner, Kemudian  data  diolah dengan bantuan komputerisasi dan diuji statistika menggunan uji Chi-square. Hasil uji statistik dengan rumus Chi Square didapatkan p value 0,02, α 0,05 sehingga Ha diterima dan H0 ditolak berarti  ada korelasi antara kejadian BBLR dengan riwayat kehamilan ibu di puskesmas rendeng kabupaten Kudus. Diharapkan Ibu hamil  lebih sering melakukan kunjungan antenatal care guna untuk mengetahui kondisi ibu dan juga janin sehingga apabila terdapat komplikasi selama kehamilan dapat terdeteksi sejak dini.
SEPSIS-LIKE SYNDROME IN A NEWBORN CHILD WITH A DISTURBANCE OF THE UREA FORMATION CYCLE
Authors: L.V. Zhdanova , E.F. Kushkoeva
Published: May 2024

Sepsis-like syndrome is a septic–like syndrome. The pathogenesis of Sepsis-like syndrome is based on the hyperproduction of proinflammatory cytokines. Hereditary metabolic disorders, including in violation of the urea formation cycle, can occur with the clinic of Sepsis-like syndrome. Ammonium easily penetrates the blood-brain barrier, causes neurotoxic effects, damages glial cells, increases osmolarity, which leads to cerebral edema. The symptoms are similar to hypoxic-ischemic lesions of the central nervous system, meningoencephalitis, intrauterine infections, which leads to a differential diagnostic search for causes and the need to exclude an infectious disease. The article presents a clinical case of a newborn with a Sepsis-like syndrome clinic. The girl was born from 2 births naturally at the age of 38 weeks. In the first day of life, there were signs of respiratory failure. She was on mixed feeding. From the 5th day of life, the deterioration of the condition caused by the syndrome of central nervous system depression, with an increase in depression of consciousness up to coma, the development of seizures. Differential diagnosis was performed with intrauterine infections, sepsis, as there was leukocytosis in the blood, increased ESR, ferritin, procalcitonin, and seeding from the cerebrospinal fluid of Micoplasma hominis. The presence of low urea content in the blood serum, up to its absence, made it possible to suspect hereditary metabolic diseases with a violation in the urea cycle. The conducted examinations revealed a total increase in the concentration of amino acids in the blood using the method of tandem mass spectrometry, metabolites in urine and hyperammonemia up to 354 mcg/dl. The newborn received antibacterial therapy, anticonvulsant therapy, sodium benzoate, and was transferred to a specialized therapeutic mixture "Nutrigen". Despite the therapy, neurological symptoms increased and the girl died at the age of 3 months. This clinical case showed the difficulties of differentiation. diagnosis of causes in the presence of Sepsis-like syndrome in newborn children.
DEVELOPMENT OF MECHANISMS TO INCREASE THE AVAILABILITY OF MEDICAL CARE IN EMERGENCIES USING GEO-INFORMATION TECHNOLOGIES
Authors: L.Yu Chernikova , D.A Boldyshev , N.L. Denisov , E.V. Karakulova
Published: May 2024

The article considers the issues of availability of reserves of medicinal products intended to provide medicinal supplies to victims in the elimination of the medical and sanitary consequences of emergencies, taking into account the internal risks for territories with complex climatic and geographical characteristics. The purpose of the study was to develop approaches to increase the territorial availability of medicinal products in the Tomsk Region reserve in emergency situations. In the course of the research, content analysis of data from regulatory documents and scientific publications on the medicinal provision of the population in emergency situations was used; correlation analysis of geo-information, spatial-geographical and natural-climatic indicators characterizing the territorial availability of medicinal products. The typologization of the Tomsk region according to the degree of transport accessibility was performed using cluster analysis algorithms. It has been established that the main climatic and geographic factors creating the internal risks for the drug supply system of the disaster medicine service of the Tomsk region include dangerous hydrological phenomena, forest and peat fires, frosts and hurricanes. More than 85% of the territory of the Tomsk region belongs to hard-to-reach areas equated by climatic conditions to those of the Far North, which poses risks for the drug supply system. Based on the data of the geo-information services, the road accessibility of the Tomsk region districts in relation to the regional center has been considered, two directions «Eastern» and « Northern» have been identified including 5 and 11 respectively. It was established that direct interaction between the districts belonging to these areas bypassing the regional center is extremely difficult. In the process of typologization of the districts according to the degree of transport accessibility in the direction "East", one group with a high degree of transport accessibility between 3 districts that are remote from the regional center has been identified. Two groups of districts have been identified in the North direction. The first group, characterized by high transport accessibility, included 5 districts, including the regional center. The second group with low transport accessibility included 6 districts. The optimal area for the placement of an additional point of medicines of the Tomsk region reserve has been justified, taking into account the transport infrastructure of the districts, natural and climatic risks, socio-demographic factors and the standard time for the organization of drug provision in an emergency situation. With the use of geo-information technologies, a model of decentralization of the reserve has been developed to increase the availability of drug care by the regional disaster medicine service of the Tomsk region in the context of internal risks.
MARKETING RESEARCH OF THE PHARMACEUTICAL MARKET OF SEDATIVE MEDICINES AUTHORIZED FOR USE IN THE TERRITORY OF THE RUSSIAN FEDERATION
Authors: T.G. Afanasyeva , A.YU. Kushnir , M.D. Novikova
Published: May 2024

In the modern world, stressful situations and stress-related disorders have become widespread among the population, representing one of the most pressing medical and social problems. Experts from around the world have traced a clear connection between stress and the development of many diseases, both from the somatic, mental and behavioral levels. Regardless of gender and age, social and cultural status, any person is susceptible to stress, and physical, emotional, informational and/or any other impact can act as a provoking factor [7,8]. When diagnosing disorders caused by stressful events, medical specialists rely on patient complaints arising at the somatic level, without taking into account the mental component, while timely treatment of ordinary stressful conditions that reduce the response adaptive functions of the body, using sedatives, will help to avoid overstrain of the body and prevent the stage of exhaustion [1,7]. The purpose of the study is to analyze the pharmaceutical market for sedative drugs registered in the Russian Federation Federation. During the research, official sources of information on the nomenclature of sedatives were used: the State Register of Medicines, the Register of Medicines and the Vidal Medicines Directory with up-to-date information for 2023. The main analysis methods used in this article are: methods of grouping, retrospective, logical, graphic and structural analysis, content analysis. As a result of the study, the main trends in the development of the range of sedative drugs were determined, and a macro-contour of the pharmaceutical market of the group under study was compiled according to the main leading characteristics of drugs.
CASE STUDY: EPISODIC PAROXYSMAL CEREBELLAR ATAXIA TYPE 2.
Authors: V.V. Miroshnikova , P.S. Krivonozhkina , A.R. Ivannikova
Published: May 2024

Instability walking is a fairly common complaint made by patients who come to be examined by a neurologist in polyclinics and hospitals. From a medical point of view, this symptom is included in the concept of "ataxia". Neurologists distinguish four types of ataxia depending on the localization of the pathological process: cortical, cerebellar, vestibular, and sensitive. There are often combinations of different types of ataxia in the same patient, for example, cortical-vestibular, sensitive-cerebellar. In addition to establishing the variant of the atactic syndrome, there is an urgent question of verifying the etiological factor that led to this pathology. Pathological heredity becomes the least obvious. In this article, we draw attention to a similar case from practice in order to form the alertness of doctors regarding hereditary coordination disorders. Episodic ataxia is a group of rare hereditary diseases of the nervous system, the main clinical manifestation of which are paroxysms of cerebellar ataxia of varying duration and frequency. The most studied from a genetic and clinical point of view are episodic cerebellar ataxia of the first and second types. The authors present a clinical case of episodic paroxysmal cerebellar ataxia type 2 in a 22-year-old patient. The diagnosis of the disease was based on a typical clinical picture and confirmed by a molecular genetic study that revealed a mutation in the CACNA1A gene. The article discusses aspects of the pathogenesis of the main forms of episodic paroxysmal ataxia, age of onset, frequency and duration of seizures, features of the clinical picture, neurological status. This clinical observation draws the attention of practitioners to the possibilities of modern methods of molecular genetic diagnosis and treatment of these conditions.
THE ASSOCIATIVE ROLE OF THE rs121908987 POLYMORPHISM OF THE PRKAG2 GENE IN THE DEVELOPMENT OF WPW SYNDROME
Authors: Yu.A. Tolstokorova , S.Yu. Nikulina , A.A. Chernova , Ya.A. Makarov , E.I. Kosharnaya , D.A Sarkisyan
Published: May 2024

The study of cardiac arrhythmias and conduction is one of the most urgent and important tasks, since these diseases are often the cause of sudden cardiac death. We assumed that the study of the contribution of "predictor genes" of heart rhythm and conduction disorders would expand knowledge about the etiology and pathogenesis of these diseases. Thus, the purpose of our research was to study the contribution of the rs121908987 polymorphism of the PRKAG2 gene to the development of WPW syndrome. Material and methods: 200 patients with VPU syndrome were examined, among them men n=97 [18:32.66;81]; women n=103 [18;47,92;83]. All patients underwent clinical, laboratory, somatometric, instrumental studies, as well as molecular genetic studies DNA isolation was performed by the standard phenol-chloroform method. Genotyping of the PRKAG2 gene was performed by PCR-PDRF analysis (polymerase chain reaction-polymorphism of restriction fragment lengths). Statistical data processing was carried out using the Excel application software package, Statistica for Windows 10.0, IBM SPSS 20.Differences in the frequency distribution of alleles and genotypes of the PRKAG2 gene between the groups were assessed using the χ2 criterion. The relative risk of disease for a specific allele or genotype was calculated as the odds ratio (OR). Results: a statistically significant predominance of the heterozygous GA genotype in patients with VPU syndrome, compared with those in the control group (8 and 2%, respectively; Table 1). There was also a statistically significant predominance of allele A carriers in the group of patients with VPU syndrome by 4%, compared with those in the control group (2%). The risk of developing PRKAG2 genotype GA syndrome, estimated by OSH, was 2.09 times higher (95% CI 1.88–2.32; p <0.001 compared with genotypes GG and AA, and in carriers of the A allele – 2.04 times higher (95% CI 1.90–2.19; p <0.001) compared with allele A (see Table 2). Conclusions: The results of the study confirmed the contribution of polymorphism rs121908987 of the PRKAG2 gene to the development of VPU syndrome.
REMOTE PHARMACEUTICAL COUNSELING USING A CHATBOT
Authors: G.A. Prosvirkin , V.V. Dorofeeva
Published: May 2024

The article discusses the use of chatbots for a variety of tasks in the medical field – such as scheduling doctor’s appointments, monitoring patients with chronic conditions, and providing healthy lifestyle recommendations – and in pharmacy operations, including verifying pharmacy addresses and hours, searching for and ordering products, and accessing information about delivery and payment, as well as operator consultations. It identifies several challenges faced in pharmaceutical counseling: time constraints, lack of motivation, absence of a dedicated counseling space, and low demand for the service. Chatbots are proposed as a solution to these issues, offering the benefit of pre-determined specific questions to address the time shortage, while the virtual nature of counseling negates the need for a physical space. Pursuant to recommended standards and legal requirements, we have developed a seven-stage model for remote pharmaceutical counseling, tailored for digital implementation. When integrated into a website, mobile app, messenger, or other online pharmacy services as a chatbot, this model enables pharmacists to consider individual client needs and provide comprehensive pharmaceutical care remotely without significantly extending consultation times. Integration with online pharmacy services is suggested to be facilitated through an API (Application Programming Interface). Furthermore, the article describes a mechanism to safeguard user personal data in the application of this algorithm.
OPTIMIZATION OF THE SURFACE OF TITANIUM DENTAL IMPLANTS OF GRADE 5 ALLOY BY BARRIER GLASS CERAMIC COATING
Authors: C.V. Novikov , I.D. Tamazov , A.I. Matveev , P.A. Topoljanskij , A.P. Topoljanskij
Published: May 2024

Pateks — the technology of applying a biocompatible glass-ceramic coating based on silicon, carbon and nitrogen on dental implants by PECVD is developed by research and production company Plasmacentre and Pavlov University (St. Petersburg, Russia). Purpose of the research — to evaluate the success of optimization of the surface of doped titanium endosseous dental implants which may be achieved with our glass-ceramic barrier coating technology using physicochemical, cytological and spectrophotometric researches. The main task is to carry out a comparative assessment of the studies’ results between dental implants with and without Pateks glass-ceramic coating (doped with titanium alloy).
Materials and methods. Titanium implants (grade 5) from BioMed were used in this research. Some of them were coated with Pateks according to our technology. A human lung fibroblast cells culture was used for the cytological study. Spectrophotometric studies were carried out on atomic absorption spectrophotometer and a atomic absorption spectrometer KVANT-Z.ETA (Russia).
Findings. Pateks-coated implants demonstrate advantage in comparison with non-coated ones in certain physicochemical parameters such as decrease of defects’ area in the initial surface of titanium implants which is formed on the previous stages of surface shaping and processing, providing higher surface hydrophilicity, increasing of dielectric characteristics and higher pH value. Pateks coating is safe for fibroblast monolayers and does not inhibit respiratory processes in them. Applying of Pateks glass-ceramic coating with a thickness of 0.5 μm reduces the content of aluminum and vanadium ions in the model medium by almost 2 times and pro- vides a barrier that helps to reduce the negative biological effect of these ions on peri-implant tissues.
MODEL OF DRUG CIRCULATION BASED ON THE DOMESTIC ANALYTICAL PLATFORM LOGINOM
Authors: S.Z. Umarov , R.A. Golubenko , A.A. Khorunzhaya
Published: May 2024

Striving to improve the economic efficiency of pharmaceutical business in an unstable market situation and high competition requires the subjects of drug circulation to implement modern approaches to the organization of their activities. In these conditions, an important problem that the management has to solve in the process of circulation of medicines is the problem of quality of source information accompanying the business processes of pharmaceutical activities. The source information is not intended for the purposes of analysis, is presented, as a rule, rather raw material that requires preliminary processing by special methods, including their sampling, purification and transformation. The article discusses the advantages of practical application of the capabilities of Loginom analytical platform, which implements the above mentioned methods of pre-processing of raw material and allows solving the tasks of modeling the characteristics of drug circulation.
Based on the concept of low-code architecture that provides visual design of complex data analysis scenarios, without involving IT-specialists and realize programming tasks by the business user.
The object of the research was the current data obtained from the accounting system of material assets of a wholesale pharmaceutical organization. When organizing a request to the accounting system, based on the considerations of commercial confidentiality, it was decided to limit the 12-month time period and the assortment group, including only antibiotics.
During the research, a data processing scenario was developed in the Loginom environment, representing the sequence of actions that need to be carried out to analyze the data. A processing script is a combination of data processing nodes configured by the developer to solve a specific problem. Each script node performs a separate operation on specific data.
The drug circulation model scenario developed during the study, consisting of five nodes, includes two nodes with initial data “Receipt Data” and “Consumption Data”, as well as nodes “Data Combination”, “Sorting” and “Grouping”.
The developed drug circulation model is an alternative to modeling based on the popular Excel spreadsheet processor, the capabilities of which are limited by the volume of processed data, low productivity on large volumes, the difficulty of automating the execution of sequences of actions, and the inconvenience of reusing processing scripts.
EVELOPMENT OF THE COMPOSITION AND TECHNOLOGY OF ANESTHETIC EYE DROPS
Authors: I.V. Alekseeva , Yu.N. Karpenko , V.V. Novikova , A.L. Golovanenko , O.A. Oleshko
Published: May 2024

In modern ophthalmological practice, the use of local anesthetics for surface anesthesia of the eyeball becomes an integral stage in preparing the patient for diagnostic studies, therapeutic manipulations, surgical and laser interventions. The share of regional pain management methods is steadily increasing. However, at the same time, the list of requirements that local anesthetics must meet is also growing. According to them, the drug that combines high anesthetic activity and minimal toxicity should be considered the best. The local anesthetic anilocaine hydrochloride, developed according to the author's method in PGFA, exhibits high surface anesthetic activity with relatively low toxicity, and also has anti-inflammatory and moderate antimicrobial activity, which distinguishes it very favorably from the local anesthetics used and is an alternative to the use of expensive imported drugs. The purpose of this study is to develop the composition and technology of anesthetic eye drops. Materials and methods. The pharmaceutical substance anilocaine hydrochloride was used as a pharmacologically active component. The determination of the hydrogen index was carried out by the potentiometric method in accordance with the requirements of the OFS "Ionometry". To study the stability of eye drops, characterized by the indicator "Related impurities", a high-pressure liquid chromatograph "ShimadzuLCProminence" (Japan) was used. Statistical processing of the results of chemical studies was carried out according to generally accepted methods using standard EXCEL computer programs. Results and discussion. As a result of the conducted research, the composition of anesthetic eye drops based on anilocaine hydrochloride has been substantiated and developed. A technology for manufacturing eye drops has been developed to ensure the sterility of the resulting form. Conclusion. Based on technological and microbiological studies, the composition and technology of locally anesthetic eye drops based on anilocaine hydrochloride for surface (drip) anesthesia of the eyeball has been developed.
STUDYING THE VALIDATION CHARACTERISTICS OF TESTING METHODS FOR AUTHENTICITY AND QUANTITATING DETERMINATION OF ACYSOLE IN DENTAL GEL
Authors: A.L. Golovanenko , E.S. Berezina , I.V. Alekseeva , O.A. Oleshko
Published: May 2024

In the quality assurance system for medicinal products, an important role is played by the procedure for validation of analytical methods at the stages of development, testing, technology and serial production. The need for validation at the stage of drug development is due to the fact that it is possible to promptly identify the shortcomings of the method and significantly improve it. The purpose of this study is to validate methods for testing «The authenticity» and «Quantitative determination» of acyzole in dental gel. Materials and methods. To achieve this goal, an active pharmaceutical substance of pharmacopoeial quality was used. When developing methods for testing for “Authenticity,” a reaction with oxalic acid to the zinc cation determined in the composition of acyzole was used. For the quantitative determination of acyzole, the complexometric method was used. The objects of the study were three series of gel samples. Results and discussion. As a result of the research, a method for testing «Authenticity» was developed; it was found that the proposed method is characterized by a negative analytical signal on model mixtures free of the analyte component and placebo, and a positive analytical signal on model mixtures of various compositions containing the analyte component. The validation characteristics of methods for the quantitative determination of acyzole in the gel were studied. positive results were obtained. The proposed methods can be used for inclusion in the regulatory documentation for the developed gel. Conclusion. When assessing the validation characteristics of the proposed methods, it was established that the methods for testing the «Authenticity» and «Quantitative determination» of acyzole in the gel are specific, the methods of quantitative determination are characterized by accuracy and repeatability, a linear dependence in the analytical range of ±20% of the declared amount of acyzole, which allows them to be used for a reliable assessment quality of the developed dental gel.
RESEARCH COMPLEXATION PROCESSES IN POKYMER BIODEGRADABLE COMPOSITIONS
Authors: D.A. Klimov , Ya.A. Poskedova , E.O. Bakhrushina
Published: May 2024

One of the possible directions of the development of targeted substance delivery systems is the creation and introduction of biodegradable polymers into production. Their distinctive feature is that they are capable of spontaneously gradually degradation in the body due to natural biochemical processes. There is an increasing spread pharmaceutical form, which use similar excipients in their technology – medicinal ocular insert. Modified cellulose, gums and rare-cross-linked acrylic polymers are used to create a biodegradable matrix base.
In terms of development approach, ocular inserts can be divided into two groups according to the principle of matrix biodegradation. In non-biodegradable composites, the effect of prolongation of the therapeutic effect is observed due to the release of the active substance from the non-decomposing matrix. This fact can be confirmed by both pharmacopoeial and non-pharmacopoeial methods. The classic tests regulated by the Pharmacopoeia of the XV edition include the "Dissolution" test (GPM.1.4.2.0014 "Dissolution of solid pharmaceutical dose forms"), and for non–pharmacopoeial ones – dialysis through a semipermeable membrane according to Kruvchinsky or using Franz's dialysis cells. The analysis of the kinetics of dissolution helps to determine and select the most suitable matrix-forming agent to be used in the composition with other substances in the composition of ocular insert.
Ocular inserts with a biodegradable matrix have a second factor that affects the release of active substances – the formation of complexes or associates between parts of the matrix and the active ingredient molecules. These intermolecular compounds can indirectly prolong the effect of the drug. All features must be taken into account in the development of ocular inserts, since they, among other things, help to model fundamentally new ways of targeted delivery of substances. In this case, it is necessary to develop a test for the second modified release factor, which would reliably determine the presence of complexation processes within the selected ocular inserts model.