bluuebunny/crossref_metadata_2025 huggingface dataset:
Chapter 9. Resilience in Research: Confronting Failure in Information Literacy Instruction
Authors: Meals Catherine
Published: July 2022

This chapter describes an academic librarian’s adoption of a research resilience philosophy toward teaching information literacy. It describes how such an approach, which focuses on the values and attitudes that are foundational to students’ development as researchers, addresses the emotional elements of information seeking, challenges in instruction formats, and guidance from academic librarians’ professional organization.
Uso de Aisladores Sísmicos Para Edificios Multifamiliares. Caso De Estudio: Edificio Multifamiliar Residencial Hungría
Authors:   , Fernández Loayza Jannet , Quispe Vilca Albert Dante , Vargas Salazar Raúl Daniel , Lucero Rojas Katherine Reyna , Alva Jiménez Alfonso Renato
Published: July 2022

Nuestro proyecto busca demostrar que se puede mejorar el desempeño sísmico de las edificaciones empleando aisladores sin afectar la rentabilidad del proyecto. Para ello, se evaluó aplicarlo en el proyecto “Residencial Hungría” el cual se trata de un edificio multifamiliar de 12 departamentos y 10 estacionamientos distribuidos en 4 pisos y un semisótano respectivamente. Se modifico la configuración original del proyecto y se incluyó un sistema de aisladores de base con núcleo de plomo con el fin de comparar la rentabilidad entre ambos proyectos (Original y modificado con aisladores). Los resultados obtenidos en la primera etapa muestran que ante un número reducido de pisos (04 pisos) la rentabilidad del proyecto modificado con aisladores es menor a la original, sin embargo, luego de evaluar múltiples opciones, se obtuvo que a partir de 07 pisos tiene un VAN de S/ 172,077 un TIR de 24 % mayor al COK que es de 20%. Sabemos que en la Filosofía y Principios del Diseño Sismorresistente contenidos en la Norma E.030 del Reglamento Nacional de Edificaciones se reconoce que dar protección completa frente a todos los sismos no es técnica ni económicamente factible para la mayoría de las estructuras, es por ello por lo que el uso de los aisladores está enfocado principalmente a estructuras del sector salud y educación. Con esto proyecto queremos propiciar la aplicación de aisladores a más proyectos de edificaciones y viviendas multifamiliares, verificando que, si puede ser rentable económicamente para el constructor, lo cual beneficiaría la demanda desatendida de la población que requiere una vivienda más segura y con mejor desempeño ante un sismo, y que minimice los costos de reparación, muertes y damnificados ante un eventual movimiento sísmico de alto impacto aun teniendo que invertir un porcentaje adicional en la compra del inmueble.
Desvelando discursos aprisionadores a partir de uma etnografia de decisões judiciais
Authors: Leôncio Chaves Paulo Victor , Sueli Rodrigues de Sousa Maria
Published: July 2022

O presente artigo busca identificar, a partir de uma etnografia de decisões aplicada sobre 914 decisões judiciais que decretaram prisões preventivas nas audiências de custódia realizadas em Teresina entre agosto/2017 e julho/2018, os fundamentos das prisões decretadas e as representações dos magistrados a respeito dos processos de criminalização e de sua função na engrenagem punitiva. Os resultados colhidos apontaram uma significativa prevalência do fundamento “para garantia da ordem publica” nas justificativas das decisões aprisionadoras (82,38% - fundamento isolado; 13,89% - fundamento cumulado; 96,28%, ao todo), ademais da presença de decisões decretadas fora das hipóteses previstas em lei. A conclusão, a partir dos resultados, é de que, no período analisado, ressalvados casos muito específicos, nenhuma prisão preventiva decretada possuiu fundamento realmente cautelar numa perspectiva de segurança endoprocessual, mas consistiram, essencialmente, em antecipações de pena, que atendem ao populismo punitivo inflamado pela mídia de massas, mas que tolhem direitos e garantias fundamentais. Evidenciou-se, ademais, que os magistrados não reconhecem os processos de criminalização como resultado da atuação seletiva dos órgãos de repressão estatal, e que, ao revés, veem-se como agentes de segurança publica, no exercício do poder punitivo, apenas.
PERTANGGUNGJAWABAN JABATAN NOTARIS SETELAH BERAKHIR MASA JABATAN TERHADAP AKTA YANG DIBUATNYA [NOTARY'S RESPONSIBILITY AFTER HIS TERM OF OFFICE ENDS ON THE DEEDS HE MADE]
Authors: Sinaga Ruth Alnila , Raffles Raffles , Suryahartati Dwi
Published: July 2022

The purpose of this study is to analyze and criticize the arrangement of the Notary's responsibility after his term of office ends and the legal consequences of the Notary's liability after the end of his term of office. This research is normative legal research and uses conceptual approach and statutory approach. The results of the research are that the responsibility of the Notary's position on the deed made by or before him is until the Notary dies because the authentic deed made by the Notary has perfect evidentiary power where the Notary knows for sure the legal actions outlined in the deed are desired and agreed upon by the parties. The legal consequences for a Notary if a legal problem arises criminally, namely if it is indicated in Article 263 of the Criminal Code to falsify a letter, criminal sanctions can be given, if there is a civil error, namely a formal error which results in the deed being an underhand deed and causing a loss, it can be requested for compensation. The loss to the deed is because it should be an authentic deed that has legal force as perfect evidence in the trial, instead it becomes an underhand deed.

BAHASA INDONESIA ABSTRACT

Tujuan dari penelitian ini adalah untuk menganalisis dan mengkritisi pengaturan pertanggungjawaban Notaris setelah masa jabatannya berakhir dan akibat hukum pertanggungjawaban Notaris setelah berakhir masa jabatannya. Penelitian ini merupakan penelitian hukum normatif dan menggunakan metode pendekatan konseptual dan pendekatan perundang-undangan. Hasil penelitian yaitu tanggung jawab jabatan Notaris terhadap akta yang dibuat oleh atau dihadapannya adalah sampai Notaris meninggal dunia karena akta autentik yang dibuat oleh Notaris mempunyai kekuatan pembuktian yang sempurna di mana Notaris mengetahui secara pasti perbuatan hukum yang dituangkan dalam aktanya dikehendaki dan disepakati oleh para pihak. Akibat hukum terhadap Notaris apabila permasalahan hukum yang muncul secara pidana yaitu bila diindikasi pada Pasal 263 KUHP melakukan pemalsuan terhadap surat dapat diberi sanksi pidana, apabila ada kesalahan perdata yaitu terhadap kesalahan formil yang mengakibatkan akta tersebut menjadi akta di bawah tangan dan menimbulkan kerugian dapat dimintakan ganti kerugian terhadap akta tersebut karena seharusnya akta autentik yang mempunyai kekuatan hukum sebagai alat bukti sempurna dalam persidangan malah menjadi akta di bawah tangan.
Intervenir sur les biocarburants et sur le stock OMC de riz du Japon pour stabiliser les prix alimentaires mondiaux
Authors: Galtier Franck
Published: July 2022

Sur les marchés internationaux, les prix des céréales (blé, maïs) et des huiles végétales (colza, tournesol, soja, palme) ont augmenté à partir de la mi-2020. Les biocarburants jouent un rôle majeur dans cette hausse et la guerre en Ukraine, qui a débuté en février 2022, l’a exacerbée. Les biocarburants lient en effet le prix de ces denrées à celui du pétrole : lorsque le prix du pétrole augmente, l’industrie des biocarburants accroît sa demande de maïs et d’huiles végétales. Limiter provisoirement cet usage industriel ferait baisser leurs prix. De plus, en cas de hausse du prix du riz, une solution serait d’autoriser le Japon à exporter son stock de riz constitué dans le cadre des règles de l’Organisation mondiale du commerce (OMC). Afin de prévenir les crises futures, ces deux leviers pourraient être déclenchés dès que les prix internationaux de ces denrées agricoles atteignent des niveaux prédéfinis.
ANALISIS KINERJA ALAT ANGKUT FORKLIF DENGAN MENGGUNAKAN METODE OVERALL EFFECTIVENESS EQUIPMENT (OEE)
Authors: Rohmatin Yuyun Yuniar , Wahyuni Rossi S.
Published: July 2022

Kegiatan material handling menjadi kegiatan penting dalam penanganan material mulai dari loading bahan baku hingga kegiatan unloading.  perusahaan x menggunakan forklif sebagai salah satu alat angkut untuk memindahkan material dalam operasi produksinya, Tingginya kerusakan pada alat angkut dapat menyebabkan turunnya produktivitas perusahaan dalam mencapai keuntungan. Metode overall equipment effectivenes (OEE) merupakan salah satu metode yang digunakan untuk mengukur kinerja peralatan industri dengan mempertimbangkan availability, performance dan rate of quality alat angkut yang digunakan, penelitian ini bertujuan untuk menghitung kinerja forklif dengan menggunakan metode overall equipment effectivenes. Berdasarkan hasil penelitian diketahui bahwa selama 6 bulan periode waktu kerusakan forklif bervariasi dengan waktu kerusakan terlama pada bulan Juni mencapai 360 menit atau setara dengan 6 jam. Nilai rata-rata availability forklif selama 6 bulan periode adalah sebesar 97,85 % dimana waktu operasi selama 6000 menit atau setara dengan 20 jam per bulan. Nilai rata-rata performance forklif adalah sebesar 97,60% dengan banyaknya piano yang berhasil diangkut rata-rata per bulan adalah sebanyak 1152 unit. Nilai rata- rata rate of quality forklif adalah sebesar 98,71 %  dengan banyaknya jumlah produk piano yang diangkut adalah sebanyak 1152 unit per bulan, sedangkan jumlah produk cacat yang gagal diangkut hanya mencapai 14,67 atau setara dengan 15 produk per bulan, Nilai kinerja forklif berdasarkan metode overall equipment effectivenes diperoleh sebesar 98,14 % dimana menurut Nakajima (1988) ketua institut perawatan jepang (JIPM) menetapkan batasan ideal dari indeks nilai OEE untuk perusahaan world class yang telah menerapkan TPM, yaitu 85% dan nilai hasil kinerja forklif perusahaan bernilai diatas nilai standar yang ditetapkan sebesar 98,14%.
PENGARUH KUAT ARUS PENGELASAN GMAW TERHADAP KEDALAMAN PENETRASI DAN KEKERASAN BAJA ST-37 PADA DAERAH HEAT AFFECTED ZONE
Authors: Fikri Agus , Setiyadi Kurniawan , Mohammad Mujirudin
Published: July 2022

Pemilihan arus yang tepat sangat berhubungan dengan kedalaman penetrasi hasil lasan pada logam, dan akan mempengaruhi sifat mekanis sambungan las yang dihasilkan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh kuat arus terhadap kedalaman penetrasi, lebar pengelasan, dan kekerasan pada heat affected zone (HAZ). Metode penelitian yang digunakan adalah eksperimental dengan kuat arus 180 A, 200 A, dan 220 A. Pengelasan dilakukan pada baja ST-37 dengan menggunakan teknik pengelasan GMAW. Kedalaman penetrasi pada komponen 1 dan 2 dengan kuat arus 180 A masing-masing sebesar 0,77 mm dan 0,78 mm, pada kuat arus 200 A sebesar 1,29 mm dan 1,06 mm, dan pada kuat arus 220 A sebesar 1,52 mm dan 1,44 mm. Lebar pengelasan yang dihasilkan untuk kuat arus 180 A adalah sebesar 5,86 mm, pada 200 A sebesar 6,70 mm, dan pada 220 A sebesar 7,35 mm. Sementara itu kekerasan daerah HAZ, menunjukkan bahwa pada kuat arus 180 A kekerasannya sebesar 122 HV, sedangkan untuk kuat arus 200 A dan 220 A kekerasannya sebesar 120,5 HV. Standar kualitas sambungan las pada masing-masing kuat arus menunjukkan bahwa kedalaman penetrasi yang dihasilkan lebih dari 20%, maka dapat disebutkan bahwa proses pengelasan sudah memenuhi standar minimal.
The ANALISIS KEKUATAN POROS DAN RODA BERSIRIP PADA ALAT PENANAM PADI PORTABEL MELALUI PENDEKATAN SIMULASI
Authors: Sukarno Ragil , Sugita I Wayan , Hartono Ahmad
Published: July 2022

Alat penanam padi otomatis sangat dibutuhkan oleh petani dalam usaha meningkatkan hasil panen. Saat ini alat penanam padi otomatis sudah ada di pasaran namun harganya cukup mahal, sehingga dibutuhkan alat penanam padi otomatis alternatif yang memiliki harga lebih terjangkau. Salah satu komponen yang sangat penting dalam alat penanam padi otomatis adalah poros dan roda, sehingga dalam penelitian ini difokuskan pada kedua komponen tersebut. Tujuan penelitian ini adalah untuk mengetahui kekuatan poros dan roda bersirip pada alat penanam pada sistem jajar legowo melalui pendekatan simulasi. Metode penelitian ini menggunakan pendekatan simulasi dengan menggunakan  perangkat lunak Autodesk Inventor yang diterapkan terhadap komponen poros dan roda alat penanam padi. Penelitian diawali dengan membuat desain rancangan model 3D, menentukan material poros dan roda, melakukan meshing, menentukan kondisi batas, menentukan posisi pembebanan, dan selanjutnya menjalankan simulasi analisis tegangan. Material poros yang digunakan adalah baja JIS G4501 Grade S45C, sedangkan material untuk roda adalah baja JIS G3101 Grade S400. Hasil simulasi menunjukkan bahwa tegangan von mises maksimal 54,90 MPa, perpindahan (displacement) maksimal sebesar 0,113 mm, serta nilai safety factor paling rendah sebesar 3,8. Hasil ini menunjukkan bahwa desain poros dan roda pada alat penanam padi telah memenuhi batas aman dan dapat dilanjutkan dalam tahap proses manufaktur.
Quality Reporting Windows May Not Capture the Effects of Surgical Site Infections After Orthopaedic Surgery
Authors: Shapiro Lauren M. , Graham Laura A. , Hawn Mary T. , Kamal Robin N.
Published: July 2022

Background:

Postoperative surgical site infections (SSIs) and the associated complications impact morbidity and mortality and result in substantial burden to the health-care system. These complications are typically reported during the 90-day surveillance period, with implications for reimbursement and quality measurement; however, the long-term effects of SSI are not routinely assessed. We evaluated the long-term effects of SSI on health-care utilization and cost following orthopaedic surgery in an observational cohort study.

Methods:

Patients in the Veterans Affairs health-care system who underwent an orthopaedic surgical procedure were included. The exposure of interest was an SSI within 90 days after the index procedure. The primary outcome was health-care utilization in the 2 years after discharge. Data for inpatient admission, inpatient days, outpatient visits, emergency room visits, total costs, and subsequent surgeries were also obtained. After adjusting for factors affecting SSI, we examined differences in each health-care utilization outcome by postoperative SSI occurrence and across time with use of differences-in-differences analysis. Cost differences were modeled with use of a gamma distribution with a log link.

Results:

A total of 96,983 patients were included, of whom 4,056 (4.2%) had an SSI within 90 days of surgery. After adjusting for factors known to impact SSI and preoperative health-care utilization, SSI was associated with a greater risk of outpatient visits (relative risk [RR], 1.29; 95% confidence interval [CI], 1.26 to 1.32), emergency room visits (RR, 1.18; 95% CI, 1.15 to 1.21), and inpatient admission (RR, 1.35; 95% CI, 1.32 to 1.38) at 2 years postoperatively. The average cost among patients with an SSI was $148,824 ± $268,358 compared with $42,125 ± $124,914 among those without an SSI (p < 0.001). In the adjusted analysis, costs for patients with an SSI were 64% greater at 2 years compared with those without an SSI (RR, 1.64; 95% CI, 1.57 to 1.70). Overall, of all subsequent surgeries conducted within the 2-year postoperative period, 37% occurred within the first 90 days.

Conclusions:

The reported effects of a postoperative SSI on health-care utilization and cost are sustained at 2 years post-surgery—a long-term impact that is not recognized in quality-measurement models. Efforts, including preoperative care pathways and optimization, and policies, including reimbursement models and risk-adjustment, should be made to reduce SSI and to account for these long-term effects.

Level of Evidence:

Economic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Implementation of Teaching Practicum for Primary School Teachers: China, Cambodia and Malawi Case Studies
Authors: Sok Saran , Gondwe Foster , Wei Liang
Published: July 2022

This paper reports findings of a qualitative study that compared the implementation of teaching practicum for primary school teachers in China, Cambodia and Malawi. The study used semi-structured interviews and document analysis. Data sources included policy documents, interviews and literature. The systems theory was employed to make explicit the implementation of teaching practicum, including processes and challenges. Findings show different approaches of teaching practicum in the three countries determined by different environmental expectations, all emphasizing providing student teachers with diverse learning experiences. The study also sheds light on some of the challenges of teaching practicum. These findings underpin the importance of local school authorities in ensuring effective teaching practicum.
THRESHOLD EFFECT IN THE RELATIONSHIP BETWEEN INFLATION RATE AND ECONOMIC GROWTH IN INDONESIA
Authors: Kusumatrisna Adam Luthfi , Sugema Iman , Pasaribu Syamsul H.
Published: July 2022

This paper investigated the linear and nonlinear relationships between inflation and economic growth in Indonesia using provincial data from 1994 to 2019. The linear model revealed that inflation has a significant negative effect on economic growth, while the nonlinear model revealed that inflation would negatively affect economic growth only after exceeding a threshold value of 9.59 percent. Excluding a high inflationarystructural break, we found an inflation threshold of 5.22 percent. Furthermore, we found that the threshold of inflation rate in the eastern regions of Indonesia was higher than that of the western regions, namely 9.64 percent and 5.75 percent, respectively. These findings have significant implications for inflation targeting and management both at the national and regional levels.
A NOTE ON PUBLIC DEBT-PRIVATE INVESTMENT NEXUS IN EMERGING ECONOMIES
Authors: Penzin Dinci J. , Salisu Afees , Akanegbu Benedict N.
Published: July 2022

We examine the effect of public debt on private investment in selected emerging economies. Using a panel threshold regression model, we estimate a threshold value of about 3 percent, on average, below which public debt stimulates private investment. Our additional analysis involving selected developed economies suggests that the crowding out effect is less evident relative to the emerging economies as higher public debt stocks do not seem to significantly undermine their private investments. These results have implications for debt sustainability and maintaining a reasonable public debt–GDP ratio is crucial for sustainable investment growth.
Seasonal Distribution of Benthic Foraminifera Associated with the Marine Sponge Liosina Paradoxa Thiele, 1899 at Sandspit Backwaters, Karachi, Pakistan
Authors: Jabeen Hina , Shafique Seema , Burhan Zaib-un-Nisa , Rasheed Munawwer , Siddiqui Pirzada Jamal Ahmed
Published: July 2022

Seasonal diversity and distribution of seven benthic foraminiferal species belonging to six families in association with the marine sponge Liosina paradoxa attached to pneumatophores of the mangrove Avicennia marina were documented at four sampling stations (St1–St4), Sandspit backwaters, Karachi coast during 2013. The calcareous species Ammonia sp. and Quinqueloculina laevigata were recorded at all stations in different seasons whereas the agglutinated species Ammotium cassis and Rotaliammina squamiformis were the most dominant species throughout the study. During the study period, water temperature ranged between 22.5–30.3°C, salinity ranged from 35–40, dissolved oxygen ranged from 0.11–3.45 mg L−1, and pH ranged from 7.15–7.69. The maximum relative abundance (45.65%) of foraminiferal species occurred at St2 from an exposed area while minimum relative abundances (13.04%) were recorded at St3 in sheltered areas. A K-dominance curve showed that highest frequency values in species were observed in the post-monsoon season as compared to the monsoon season. The present study is the first to report the foraminiferal assemblages associated with a marine sponge (Liosina paradoxa) from the mangrove habitat at Sandspit backwaters, Karachi.
Strategi Pemasaran Holistik Bank Syariah dalam Meningkatkan Kualitas Layanan E-Banking
Authors: Muhammad Awalul Akhyar  , Aji Damanuri
Published: July 2022

The development of technology has radically changed the banking transaction system, which altered the banking culture. The public has responded positively to e-banking services as a safe, reliable, and efficient payment system solution. Banking institutions such as Islamic banks need to have a holistic and standardized marketing strategy to be implemented in e-banking service products so that Islamic banks can survive in the current era of technological development. This research relates to the holistic marketing strategy of Islamic banks in improving the quality of e-banking services. This study aims to describe and analyze the strategy, tactics, and values ​​that Bank Syariah Mandiri Ponorogo Branch Office uses to improve the quality of e-banking services to realize quality e-banking marketing and services.
Putusan Open Legal Policy Ketentuan Tindak Pidana Zina Perspektif Maqashid Syariah
Authors: Amiruddin Hasan  , Abid Rohmanu
Published: July 2022

Constitutional Court Decision No. 46/PUU-XIV/2016 which rejects the expansion of the meaning of adultery in the Criminal Code has become a hotly debated topic. Some people think that the Constitutional Court should have the courage to make legal breakthroughs on this very important issue. However, the Constitutional Court Justices are of the opinion that the expansion of the meaning of adultery is not within their jurisdiction.

This type of research is a normative juridical research, with a statute approach. The nature of the research is descriptive analytical, using a knife of maqa>s{id sharī'ah concept analysis.

From the results of the research, it was concluded that first, the contextualization of Jaseer Auda's maqa>s{id sharī'ah concept against the arguments of the Constitutional Court judges in family resilience was actually quite well represented. However, the judges tended to choose the cause of the damage to be more due to the existence of a void in the law, in which the authority to fill in the law could only be exercised by the Indonesian Legislation Body, namely the Indonesian House of Representatives. Second, LGBT are the most massive and dangerous implications indirectly in the decision of the open legal policy. In addition to the harm in the form of diseases caused by these actions, the threat of nasab (hifdz al-nasab) which is guarded by Islam itself and the tarnishing of honor that is contrary to Islamic teachings in order to maintain the honor of oneself and others (hifdz al-'irdh). Third, Realistic efforts to prevent cohabitation and the potential for violence against women, namely through the contemporary maqa>s{id system approach, include the following, namely by limiting the facilities that are feared to cause convenience in cohabitation behavior.
The beneficial clinical effects of teriflunomide in experimental autoimmune myasthenia gravis and the investigation of the possible immunological mechanisms
Authors: Koseoglu Emel , Sungur Neslihan , Muhtaroglu Sabahattin , Zararsiz Gokmen , Eken Ahmet
Published: July 2022

Myasthenia Gravis (MG) is an autoantibody-mediated autoimmune disease characterized by skeletal muscle weakness exacerbated with exercise. There is a need for novel drugs effective in refractory MG patients. We aimed to test the potential of teriflunomide, an immunomodulatory drug currently used in rheumatoid arthritis and multiple sclerosis treatment, in a murine experimental autoimmune myasthenia gravis (EAMG) model. EAMG was induced by immunizations with recombinant acetylcholine receptor (AChR). Teriflunomide treatment (10 mg/kg/day, intraperitoneal) was initiated to one group of mice (n=21) following the third immunization and continued for five weeks. The disease control group (n=19) did not receive medication. Naïve mice (n=10) received only mock immunization. In addition to the clinical scorings, the numbers of B cells and T cells, and cytokine profiles of T cells were examined by flow cytometry. Anti-AChR specific antibodies in the peripheral blood serum were quantified by ELISA. Teriflunomide significantly reduced clinical disease scores and the absolute numbers of CD4+ T cells and some of their cytokine producing subgroups (IFN-γ, IL-2, IL-17A, GM-CSF) in the spleen and the lymph nodes. The thymic CD4+ T cells were also significantly reduced. Teriflunomide mostly spared CD8+ T cells’ numbers and cytokine production, while reducing CD138+CD19+ lambda+ plasma B cells’ absolute numbers and CD138 mean fluorescent intensities, probably decreasing the number of IgG secreting more mature plasma cells. It also led to some selective changes in the measurements of anti-AChR specific antibodies in the serum. Our results showed that teriflunomide may be beneficial in the treatment of MG in humans.
Radioprotective effect of nanoniosome loaded by Mentha Pulegium essential oil on human peripheral blood mononuclear cells exposed to ionizing radiation
Authors: Hamzian Nima , Nickfarjam Abolfazl , Shams Ali , Haghiralsadat Fateme , Najmi-Nezhad Moslem
Published: July 2022

Introduction
: Despite the applications and advantages of ionizing radiation; there are many radiation risks to biological systems that are necessary to be reduced as much as possible. The present study aimed to assess the radioprotective effect of nanoniosomes loaded by Mentha Pulegium essential oil (MPEO-N) as a natural antioxidant on human peripheral blood mononuclear cells (PBMCs).
Materials and methods
MPEO-N nanoparticles were prepared by the lipid thin-film hydration method, and its physicochemical characteristics were analyzed. PBMCs were then irradiated with X-ray using a 6 MV linear accelerator at radiation doses of 25 and 200 cGy in the presence of non-toxic concentrations of MPEO-N nanoparticles (IC10). After 48 and 72 h of incubation, the radioprotective effect was investigated by measuring survival, apoptosis, and necrosis of PBMCs, using MTT assay and flow cytometry analysis.
Results
The hydrodynamic diameter and zeta potential of nanoniosomes were 106.0 nm and − 15.2 mV, respectively. The mean survival percentage of PBMCs showed a significant increase only at a radiation dose of 200 cGy compared with the control group. The percentages of apoptosis and necrosis of cells in the presence of MPEO-N nanoparticles at both radiation doses and incubation periods (48 and 72 hours) demonstrated a significant reduction compared with the control.
Conclusion
MPEO-N nanoparticles as a natural antioxidant, exhibited a favorable radioprotective effect by a significant reduction in the percentage of apoptosis and necrosis of irradiated PBMCs.
Effect of miR408 on photosynthesis of rice under enhanced UV-B radiation
Authors: Ji Bing-Xuan , Li Dan , Wang Lin-Ye , Lv Chuan-Gen , Ji Li-Rong , Zhang Qi-Jun , zhang meiping
Published: July 2022

UV-B stress has become one of the main abiotic stress factors threatening crop growth and development, and miR408 plays an important role in plant abiotic stress response. MiR408 is a type of endogenous non-coding small fragment single-stranded RNA, which is mainly found in eukaryotes and participates in the regulation of plant growth and development by responding to various stresses in a manner that negatively regulates the target gene. The effects of photosynthesis and expression levels of miR408-OE rice plants and knockout UCL8 plants under UV-B stress were investigated. The results showed that under UV-B enhanced radiation, the chlorophyll content, intercellular carbon dioxide concentration, net photosynthetic rate and stomatal conductance of the overexpressed miR408 plants were significantly reduced. Under UV-B enhanced radiation, chlorophyll fluorescence kinetic parameters such as Fv/Fm and qP overexpressing miR408 plants were reduced. UV-B significantly inhibited the activity of SOD, POD and CAT in overexpressing miR408 plants and significantly increased their H2O2 and MDA contents. Under UV-B stress treatment, the yield traits such as the number of grains, the number of tillers and the quality of dry matter of overexpressing miR408 plants were significantly reduced. Therefore, UV-B enhances the photosynthetic rate, photosynthetic performance and disruption of the antioxidant system balance of overexpressing miR408 plants, which leads to a decrease in yield. It is speculated that overexpression of miR408 reduced the tolerance of rice to UV-B radiation, that is miR408 may play a negative regulatory role in response to UV-B stress.
Reduced sensitivity of commercial Spike-specific antibody assays after primary infection with the SARS-CoV-2 Omicron variant
Authors: Springer David Niklas , Perkmann Thomas , Jani Claudia , Mucher Patrick , Prüger Katja , Marculescu Rodrig , Reuberger Elisabeth , Camp Jeremy Vann , Graninger Marianne , Borsodi Christian , Deutsch Josef , Lammel Oliver , Aberle Stephan Walter , Puchhammer-Stöckl Elisabeth , Haslacher Helmuth , Höltl Eva , Aberle Judith Helene , Stiasny Karin , Weseslindtner Lukas
Published: July 2022

The SARS-CoV-2 Omicron variant is characterized by substantial changes in the antigenic structure of the Spike (S) protein. Therefore, antibodies induced by primary Omicron infection lack neutralizing activity against earlier variants. In this study, we analyzed whether these antigenic changes impact the sensitivity of commercial anti-SARS-CoV-2 antibody assays. Sera from 37 unvaccinated, convalescent individuals after putative primary Omicron infection were tested with a panel of 20 commercial anti-SARS-CoV-2 immunoassays. As controls, we used samples from 43 individuals after primary infection with the SARS-CoV-2 ancestral wildtype strain. In addition, variant-specific live-virus neutralization assays were used as a reference for the presence of SARS-CoV-2-specific antibodies in the samples. Notably, in Omicron convalescents, there was a statistically significant reduction in the sensitivity of all antibody assays containing S or its receptor-binding-domain (RBD) as antigens. Furthermore, antibody levels quantified by these assays displayed a weaker correlation with Omicron-specific neutralizing antibody titers than with those against the wildtype. In contrast, the sensitivity of nucleocapsid-protein-specific immunoassays was similar in wildtype and Omicron-infected subjects. In summary, the antigenic changes in the Omicron S lead to reduced immunoreactivity in the current commercial S- and RBD-specific antibody assays, impairing their diagnostic performance. Importance This study demonstrates that the antigenic changes of the SARS-CoV-2 Omicron variant affect test results from commercial Spike- and RBD-specific antibody assays, significantly diminishing their sensitivities and diagnostic abilities to assess neutralizing antibodies.
A 4-factor perspective of the Patient-Practitioner Orientation Scale (PPOS): A deeper understanding of patient-centeredness
Authors: Jiang Yinan , Wei Jing , Shi Lili , Cao Jinya , Zhu Boheng , Hong Xia
Published: July 2022

Background: Although patient-centered medical services are widely recognized and accepted, how to define and evaluate them remains controversial. Objectives: This study attempts to evaluate the underlying structure of PPOS with a homogenous population and clarify the connotation of patient-centeredness. Methods: In this cross-sectional study, 279 Chinese medical students in grade 7 were selected to examine the internal structure of PPOS by means of internal consistency, exploratory factor, and confirmatory factor analyses. Results: Both the two-factor model and the four-factor model showed acceptable internal consistency and structural validity. The four-factor model that endorsed the implicit attitude towards doctor-patient relationship outperformed the two-factor model in terms of adaptability. Conclusions: PPOS has good psychometric attributes in Chinese medical students, and the patient-centered evaluation should pay more attention to the implicit attitude towards doctor-patient relationship. Practice implications: Understanding the underlying attitudes towards doctor-patient relationship can help to construct a patient-centered medical service concept and improve doctor-patient relationship in the course setting of medical education and the system design of medical activities.
Computational Intelligence Approach to Improve the Classification Accuracy of Brain Neoplasm in MRI Data
Authors: Sinhababu Nilanjan , Samanta Ananya , Samanta Debasis
Published: July 2022

Of late, in many medical diagnostic applications, automatic detection of brain neoplasm in Magnetic Resonance Imaging (MRI) data is gaining importance. This report presents two improvements for brain neoplasm detection in MRI data: 1) an advanced preprocessing technique to accurately identify the region of interest in MRI data and 2) a hybrid technique using Convolutional Neural Network (CNN) for feature extraction followed by modified Support Vector Machine (SVM) for classification. Toward the advanced preprocessing, contour calibration, Sobel edge detection and contrast optimization, which were ignored in the existing works have been investigated in this work. The autoencoder of CNN model has been used to extract the feature automatically in contrast to the manually defined feature as in the conventional techniques. The learning algorithm for SVM is modified with the addition of a cost function to minimize the false positive prediction addressing the errors in MRI data diagnosis. The proposed approach efficiently detects the presence of neoplasm and also predict whether it is malignant or benign. To check the effectiveness of the proposed preprocessing technique, it was inspected visually and then evaluated using the performance of prediction accuracy. A comparison study between the proposed classification technique and the existing techniques was performed. The result showed that the proposed approach outperformed in terms of accuracy and can handle errors in classification better than the existing approaches.
Agreement between a mobile applet-based visual acuity self-test program and the conventional method for distance and near visual acuity test
Authors: Xian Yiyong , Ye Yuhao , Liu Fang , Zhou Xingtao , Zhao Jing
Published: July 2022

PURPOSE: To investigate the agreement between a mobile applet-based visual acuity (VA) self-test program and the conventional VA tests.METHODS: This consecutive case series study included 121 children and adults (242 eyes). Patients were classified into three groups according to age (children, adolescents, and adults). They underwent uncorrected distance visual acuity (UDVA) testing, distance visual acuity with frame spectacle (DVA with FS) testing at 2.5-m distance, uncorrected near visual acuity (UNVA) testing, and near visual acuity with frame spectacle (NVA with FS) testing at a 0.4-m distance using a mobile applet-based VA self-test program and conventional VA tests in two eyes. RESULTS: Correlations among UDVA, DVA with FS, UNVA, and NVA with FS between the two methods were significant in all subjects (all P<0.001). The intraclass correlation coefficient were 0.960. 0.845, 0.960, and 0.669, respectively (all P<0.001). The proportions of bias outside the 95% confidence interval limit of agreements were 6.20%, 4.82%, 7.08%, and 6.10%, respectively. There were significant differences in NVA with FS between the two methods (P<0.05) in the adolescent group, but no differences in measured parameters were found among children and adult groups.CONCLUSIONS: There is good agreement between the mobile applet-based VA self-test program and the conventional VA tests. The VA self-test has good practical value especially in the current pandemic, allowing self-screening of visual acuity, myopia control, and remote management of visual impairment in oculopathy.
Hypercholesterolemia promotes the intravasation of breast tumor cells through an LDL-LDLR axis
Authors: Magalhães Ana , Cesário Vanessa , Coutinho Diogo , Matias Inês , Domingues Germana , Pinheiro Catarina , Serafim Teresa , Dias Sérgio
Published: July 2022

Majority of metastasis in breast cancer occur via the dissemination of tumor cells through the bloodstream. How tumor cells enter the blood (intravasation) is, however, a poorly understood mechanism at the cellular and molecular levels. Particularly uncharacterized is how intravasation is affected by systemic factors. High levels of systemic LDL-cholesterol have been shown to contribute to breast cancer progression and metastasis in various models, but the mechanisms involved are still undisclosed. Here we show that a high cholesterol diet promotes intravasation in two mouse models of breast cancer and that this could be reverted by blocking LDL binding to LDLR in tumor cells. Moreover, we show that LDL promotes vascular invasion in vitro and a phenotypic change resembling VM, and augments the expression of Serpine2, previously shown to be required for both VM and intravasation. Finally, we show that blocking the binding of LDL to LDLR on tumor cells prevents the increase in lung metastasis promoted by a high cholesterol diet. Overall, our manuscript unravels novel mechanisms by which systemic hypercholesterolemia may affect the onset of metastatic breast cancer by favoring phenotypic changes in breast cancer cells and increasing intravasation.
P53 transcribes GSDMD to induce delayed pyroptosis in radiation-induced intestinal injury
Authors: Liu Tingting , Dong Suhe , Lin Yuhan , Liu Ruling , Yang Yajie , Yao Liuhuan , Du Jicong , Gao Fu , Liu Cong , Hu Hao , Liu Hu
Published: July 2022

Background
radiation-induced intestinal injury (RIII) is an important cause of death in nuclear accidents and common complication after radiotherapy in patients with pelvic, abdominal, or retroperitoneal tumors. Up to now there is no effective means to prevent or treat RIII due to its complex mechanism, in which the death of intestinal cells is the main reason. Recently, GSDMD-mediated pyroptosis was identified as an important type of cell death and play a role in many diseases. However, the effect of pyroptosis on RIII is still unclear.
Method
using GSDMD knockout mice, the role of pyroptosis in the RIII was investigated. By detecting the release of LDH, expression of GSDMD, Caspase-11, Caspase-1 and absorption rate of SYTOX Green, the pyroptosis of radiated Mode-k cells was determined, simultaneously the common pyroptosis induced by LPS was conducted as positive control. Further, the upstream of GSDMD were screened by predictive analysis of transcription factors combing RNA-seq.
Results
we showed that GSDMD-mediated pyroptosis is involved in the process of RIII, and unexpectedly found that radiation induced a delayed pyroptosis that is substantially different from common pyroptosis induced by such as LPS. Further investigation revealed that radiation-induced DNA damage up-regulated the expression of P53, which subsequently transcribes GSDMD. In addition, the up-regulated GSDMD led to pyroptosis simultaneously promoting Ca2+ influx that afterwards enhanced apoptosis induced by radiation. Finally, targeting GSDMD, disulfiram displayed a potential protection for RIII.
Conclusion
radiation could induce delayed pyroptosis in the intestinal epithelial cell that is greatly different from common pyroptosis. During that process, GSDMD was cleaved and had inducible high expression which was mainly mediated by P53 transcription.
The role of farm subsidies in changing India’s water footprint
Authors: Chatterjee Shoumitro , Lamba Rohit , Zaveri Esha
Published: July 2022

Dwindling groundwater supplies threaten food security and livelihoods. Farm subsidies which distort production decisions are an ubiquitous agricultural policy tool, yet their contribution to growing groundwater stress remains poorly quantified. Here we show how output subsidies that guarantee purchase of crops at higher than market prices have contributed substantially towards declining water tables in India. Overall, these policies have led to a 30% over-production of water intensive crops. In the northwestern state of Punjab, rice procurement accounts for 63% of the rise in groundwater declines over two decades. In the central state of Madhya Pradesh, wheat procurement adopted in the late 2000s has driven a 5.3 percentage point increase in dry wells and a consequent 3.4 percentage point increase in deep tubewells. The results demonstrate how well intentioned but poorly designed subsidies can impose harmful externalities on the environment and undermine long-term sustainable development.
Blood plasma high-density lipoprotein remodeling as a reserve mechanism for goldfish adaptation to critical salinity conditions
Authors: Andreeva Alla , Lamash Nina , Martemyanov V.I. , Vasiliev A.S. , Toropygin I.Yu. , Garina D.V.
Published: July 2022

In goldfish Carassius auratus acclimated under critical salinity conditions (CS, 11.5 g kg -1 ), the concentrations of cortisol, glucose, Na + in the blood plasma increased significantly, also large β - HDL particles decomposed into small α- ones with a higher negative charge and a smaller number of apolipoproteins molecules in 1 particle composition. Particles remodeling led to ~1.5-2-fold increase in their number per unit plasma volume. Taking into account that under CS conditions the contribution of organic osmolytes to plasma osmolality increased by ~2%, and the content of the bound water fraction in plasma increased by ~4 times, we assumed that goldfish have a “reserve” mechanism for maintaining homeostasis: when the possibilities of increasing the concentration of Na + and organic metabolites in plasma are limited, remodeling of HDL particles and an increase in their amount in plasma can help to maintain its hyperosmotic status and additional influx of water into the body.
Analyses on safety and efficacy of nonstandard dose of r-tPA in intravenous thrombolysis treated AIS patients
Authors: Yuan Jiawen , Wu Ruxing , Xiang Jingyan , Deng Jiangshan , Zhang Xiaojie , Lu Kaili , Cao Fengya , Zhao Fei , Zhao Yuwu , WANG FENG
Published: July 2022

Background The widely recommended standard dose of recombinant tissue plasminogen activator(r-tPA) is 0.9mg/kg. Practically, the dose of r-tPA is still a topic that is constantly being discussed.Methods For this observational study, data were obtained from 537 patients who received r-tPA thrombolysis in Shanghai Sixth People’s Hospital stroke center in five years (2014-2019). Patients were divided into two groups: nonstandard dose group (0.6mg/kg ≤ dose < 0.9mg/kg) and standard dose group (0.9mg/kg). Different outcomes were observed: efficacy: 3 months mRS 0-1 (3m-mRS0-1); safety: symptomatic intracranial hemorrhage within 24 hours (24h-sICH) and 3 months mortality (3m-death). We also observed the effect of r-tPA dose coefficient on outcomes in different age and baseline National Institute of Health stroke scale (NIHSS) score subgroups. Results There are 265 patients given standard dose treatment, and 272 given nonstandard dose. There was no significant difference between non-standard dose group and standard dose group in 3m-mRS0-1, 3m-death and 24h-sICH (p=0.567, 0.327 and 0.415 respectively). The dose coefficient present a significant negative correlation (p=0.034, B=-4.290) with 3m-death in NIHSS <16 sub-group. Door to needle time (DNT) is the most important independent outcome-influential factor (MIOIF) in NIHSS ≥16 sub-group. The diabetes history and baseline NIHSS were the MIOIF in the age ≥80 year sub-group.Conclusions The non-standard dose group was as safe and effective as the standard dose group in our study. While, in NIHSS<16 group, lower doses seemed to be relative to 3m-death. Therefore, the standard dose should still be the first choice in r-tPA treated AIS patients.
Analysis of the efficacy of LNSRH for early cervical cancer based on T, B and NK lymphocytes
Authors: He Lijie , Ma Liangliang , Zhang Heping
Published: July 2022

Objective The analysis is based on the analysis of T, B, NK lymphocytes to analyze the efficacy of LNSRH surgery in the treatment of early cervical cancer. Methods This article selected 30 patients with early cervical cancer who underwent LNSRH surgery in our hospital from December 2019 to January 2021 as the cervical cancer group, and 30 healthy women who underwent physical examination in our hospital during the same period were selected as the control group. The expressions of CD4+, CD8+, CD20+, and CD57+ were detected by flow cytometry, FSFI, FACT-G scores were used to evaluate female sexual function and quality of life, and the receiver characteristic curve (ROC) was used to analyze the expression of CD4+, CD8+, CD20+, and CD57+ in LNSRH surgery The predictive value of curative effect in the treatment of early cervical cancer. Results The expression of CD4+ and CD57+ in the cervical cancer group was lower than that in the healthy group, and the expression of CD8+ and CD20+ was higher than that in the healthy group (P<0.05). Compared with preoperatively, the expression of CD4+ and CD57+ was higher after operation, and the expression of CD8+ and CD20+ was lower (P<0.05); compared with 1 day after operation, the expression of CD4+ and CD57+ was higher at 3d postoperatively, and the expression of CD8+ and CD20+ was lower (P<0.05). CD4+, CD57+ expression was positively correlated with FSFI score, CD8+, CD20+ expression was negatively correlated with FSFI score, CD4+, CD57+ expression was positively correlated with FACT-G score, and CD8+, CD20+ expression was negatively correlated with FACT-G score (P<0.05) . ROC analysis of CD4+, CD8+, CD20+, CD57+ expression sensitivity was lower than the four-item combination, and the specificity was higher than the four-item combination, with statistical difference (P<0.05). The four combined tests have good predictive value for the efficacy of LNSRH surgery in the treatment of early cervical cancer (P＜0.05). Conclusion Cervical cancer patients are mainly cellular immunity. Cervical cancer patients have low expression of CD4+, high expression of CD8+, high expression of CD20+, and low expression of CD57+. T, B, and NK lymphocytes can be used for immunological detection of cervical cancer.
Direct anterior versus posteriorlateral approachs for clinical outcomes after total hip arthroplasty in the treatment of severe DDH
Authors: Lan Yiping , Lin Bei , Feng Eryou , Lu Zhiming , Lin Feitai , Weng Yan
Published: July 2022

Background
The total hip arthroplasty (THA) has gained popularity in in the treatment of severe developmental dysplasia of the hip(DDH).the posterior lateral approach (PLA) has good clinical efficacy and has been confirmed by the majority clinicians.Nevertheless, controversy exists regarding longer-term benefits of the direct anterior approach(DAA) .The objective of this study was to investigate the clinical efficacy and placement of S-ROM prosthesis in the treatment of severe DDH by The total hip arthroplasty (THA) with different surgical approaches.
Methods
A retrospective analysis was performed on 41 patients with severe DDH admitted to our hospital from August 2015 to February 2022, who were treated with S-ROM prosthesis for total hip arthroplasty and subtrochanteric osteotomy of the femur. They were divided into DAA group and PLA group according to different surgical approaches. Perioperative indicators and imaging data were collected.
Results
The surgery time,intraoperative blood loss, and creatine kinase difference in DAA group and PLA group was without a statistically significant difference (P > 0.05). The postoperative length of hospitalization was shorter in the DAA group than in the PLA group (6.50 ± 3.15 vs 9.18 ± 4.93,P = 0.045).The acetabular abduction angles、the acetabular anteversion angles, the safe area ratio, The difference of femoral eccentricity, and the vertical difference of ofrotation center in DAA group and PLA group, there was no statistical significance (P > 0.05).Statistically signifificant differences were detected the horizontal difference of ofrotation center(P = 0.044).
Conclusions
Total hip arthroplasty with S-ROM prosthesis is a feasible procedure for severe dysplastic DDH. The clinical efficacy and prosthesis placement parameters of DAA approach are advanctage to those of PLA approach.