bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer
Authors: Peng Jiefei , Bu Fan , Duan Lei , Song Anna , Wang Guojun , Zhang Zhijun
Published: October 2024

Background

Colorectal cancer (CRC) is a leading cause of morbidity and mortality, and timely diagnosis and intervention are crucial for cancer patients. Transfer RNA-derived fragments (tRFs) play a noncoding regulatory role in organisms. Serum EV(extracellular vesicles), as an integral mediator of intercellular transmission of genetic information vesicles in Transfer RNA-derived fragment (tRF RNA), are expected to be minimally invasive diagnostic and predictive biologic factors of CRC.

Methods

Collect serum samples from 205 CRC patients, and then isolate extracellular vesicles from the serum. Captured the physical morphology of EV through transmission electron microscopy. The particle size was detected by particle size assay, and protein expression on the surface of EV was verified by Western blot. Gene microarrays were screened for differentially expressed tRF-RNA. TRF RNAs were verified by qPCR for differential expression in 205 CRC patients and 201 healthy donors, assessing the CRC diagnostic efficiency by area under the curve (AUC).

Results

Compared with 201 healthy donors, CRC patients experienced significantly down-regulated serum EV 3’tRF-ThrCGT while significantly up-regulated 3’tRF-mtlleGAT. Serum EV 3’tRF-ThrCGT and 3’tRF-mtlleGAT predictive diagnostic efficiency: 0.669 and 0.656, and the combination of CEA and CA724 predictive diagnostic efficiency was 0.938.

Conclusion

The study data showed that 3’tRF-ThrCGT and 3’tRF-mtlelGAT can be minimally invasive diagnostic CRC indicators. The combination of tumor markers CEA and CA724 has important diagnostic significance.
Osmolytes and CsAQP expression jointly influence water physiology in the peel and pulp of orange (Citrus sinensis (L.) Osbeck) fruit during postharvest water loss
Authors: Lin Xiong , Wei Qingjiang , Zeng Lingcai , Zhan Minxuan , Li Feng , Chen Jinyin , Ma Qiaoli
Published: October 2024

Water loss is a serious issue affecting the quality of postharvest horticultural products. Aquaporins (AQPs) regulate the transport of water across biological membranes, along the gradient of water potential, and may play a role in water loss. In this study, matured orange fruits (Citrus sinensis) stored at ambinent temperature (RH 85-95%) for 105 d showed that the weight loss persistently increased, and its rate peaked at 45–60 d and 90–105 d. Both water content and potential were higher in the pulp than in the peel. Water content rose before 60 d, and peel water potential fell with an increased gradient after 60 d. Comparing with peel, osmolytes such as soluble sugar, sucrose, glucose, fructose, and organic acids showed higher accumulation, and their levels were the lowest around 60 d. In contrast, soluble protein and inorganic minerals showed low levels of accumulation in the pulp. In total, 31 CsAQP genes were expressed in the fruit, and most of them were down-regulated in the peel but up-regulated in the pulp during storage. These genes were subsequently classified into four clusters based on their expression patterns. Genes in Cluster I — including CsNIP1;1/2;1/2;2/2;3/3;1/4;1/6;1, CsTIP1;3/2;2/2;3/5;1/6;1, CsXIP1;1/1;2, CsSIP1;2, and CsPIP1;2 — were persistently up-regulated in the pulp for the 105 d of storage, especially at day 60, when some genes showed 103-fold higher expression. Pearson’s correlation and principal component analysis further revealed a significant positive correlation among weight loss rate, water content, and water potential gradient (R2 = 0.85). Indexes positively correlated with osmolyte content and Cluster I gene expression in pulp samples suggest that increased CsAQP gene expression in pulp is linked to faster water loss in oranges, particularly at 60 days postharvest.
The impact of the herd health interventions in small ruminants in low input production systems in Ethiopia
Authors: Moliso Mesfin Mekonnen , Molla Wassie , Arke Asrat , Nana Tesfalem , Ayele Zewudie Firdawok , Tibebu Abebe , Haile Aynalem , Rekik Mourad , Magnusson Ulf , Wieland Barbara , Knight-Jones Theodore
Published: October 2024

Introduction

Diseases have a negative impact on production and profitability of small ruminants. A good herd health program can decrease the number of sick animals and improve herd performance.

Methods

In a longitudinal study, small ruminant herd health interventions such as community-based strategic gastrointestinal (GI) parasite control, prevention and control of major respiratory diseases and capacity development activities were implemented. In four districts of Ethiopia, where the Community Based Breeding Program (CBBP) is implemented, morbidity and mortality data were collected from January 2018 to July 2021 in 1047 smallholder farms with the objective of evaluating the impact of herd health interventions. A total of 2,643 sick animals and 516 deaths of small ruminants were recorded during the study period. The disease cases were categorized into eight groups: gastrointestinal, neurological, reproductive, respiratory, skin, systemic, other diseases (eye disease, foot disease etc) and unknown diseases. Chi-square and proportions were used to analyze morbidity and mortality by district, agro-ecological zone and age of the animal.

Results

The data showed that the general trend in the occurrence of cases and morbidity rate were decreasing from 2018 to 2021 in intervention villages. Overall, the morbidity rate in young animals (7.36%) was highier than in adults (3.49%) and the mortality rate difference between young and adult animals was also statistically significant (p < 0.001). The morbidity and mortality rates varied significantly (p < 0.001) among districts and among agro-ecologies. According to the data, treating and following up of infected animals reduced the mortality rate significantly. The relative risk of death in treated animals after the case reported was 0.135. Generally, the intervention impact analysis revealed that morbidity rate was decreased in intervention years (6.31% in 2018 to 3.02% in 2021) and that herd health interventions provide an added value.

Conclusion

Generally, herd health intervention had significant impact in reducing the morbidity rates in years and treatment and follow up of sick animals due to early reporting reduced mortality rate significantly. It is recommended that the herd health management should be the core activity under small ruminant production programs.
Integrated AGC Approach for Balancing the Thickness Dynamic Response and Shape Condition of a Hot Strip Rolling Control System
Authors: Huang Yu-Chan , Wu Tung-Chin , Chien Wei-Yi , Tsai Min-Che , Peng Chao-Chung
Published: October 2024

Thickness and shape are two significant indices in the tandem hot strip rolling process. Excessive thickness correction can cause rolling force imbalance, leading to deteriorated shape conditions. Aiming at this problem, we propose a new monitor automatic gauge control (M-AGC) with consideration of the rolling force distribution to not only keep the shape condition but also improve the robustness of the rolling process. First, the M-AGC with the Smith predictor control structure was studied. Second, based on the rolling force and thickness correction redistribution algorithm, shape balance automatic gauge control (SB-AGC), the phenomenon of rolling force imbalance was improved. Third, the transient response was improved by adding a command prefilter to the upstream stands, which is asynchronous shape balance automatic gauge control (ASB-AGC). The proposed algorithms can be applied to all steel grades and are easy to implement. Finally, cross-validation was conducted through numerical simulations and application results, confirming the alignment between the theoretical derivation and practical implementation. The simulation results show improvements in both the rolling force balance and the thickness response. The practical application results also confirm that better flatness conditions and thickness response can be achieved, significantly reducing the amount of head-end cutoff losses.
Actuators for Large Wind Energy Systems—A Tutorial-Focused Survey
Authors: Gambier Adrian
Published: October 2024

Undoubtedly, wind turbines are currently one of the most significant contributors to clean energy. Therefore, it is crucial to enhance the capability of wind turbines, which in turn leads to an increase in their dimensions. Nevertheless, only advanced control systems can guarantee the optimal and secure operation of these huge machines. On the other hand, the precise control of these modern wind turbines is only achievable through the use of highly specialised actuators. Despite their importance, actuators have historically been overlooked and seen as secondary components in control systems. However, in modern machines, actuators are required to manipulate multiple tonnes or manage thousands of volts and amperes within short times. Consequently, greater emphasis must be placed on their handling and operation. This study aims to review actuators for modern large wind energy converters from a control engineering perspective, using a tutorial approach.
Telework and Women’s Perceptions on the Right to Disconnect—An Exploratory Study in Portugal
Authors: Rebelo Glória , Delaunay Catarina , Diamantino Maria Fernanda , Almeida António R.
Published: October 2024

Working in the digital age requires a discussion on the right to disconnect. Although it has previously been studied in association with the digital transition movement, the “right to disconnect” has gained relevance in a context of mandatory teleworking due to the COVID-19 pandemic. This situation has led some countries to legislate on the subject, notably Portugal, where the right to disconnect has been enshrined in labour legislation since law no. 83/2021 of 6 December. This article presents a framework of the literature on the right to disconnect, as well as a documentary analysis and an exploratory study carried out in Portugal in November and December 2021, during the COVID-19 pandemic. The survey sought to assess the working conditions of women in telework, particularly about working time. This study stresses that the right to disconnect is linked to the organisation of working time and analyses the negative impact of technology on work, in particular the permanence of the electronic connection to work. The results show that the majority of women value teleworking because they have more time for themselves and their families. However, the women who consider that they have less availability for teleworking indicate that the main reason for this is not being able to disconnect from work. In the context of the digital transition and the expansion of teleworking in organisations and the generalisation of hybrid work, the study of this new “right to disconnect” becomes crucial.
Guidance, Navigation, and Control for the Moon, Mars, and Beyond
Authors: Sagliano Marco
Published: October 2024

The interdisciplinary field known as Guidance, Navigation, and Control (GNC) has been one of the key contributors to the tremendous advancements in space exploration since the inception of the Mercury and Apollo programs [...]
Effects of Rainfall and Harvest Time on Postharvest Storage Performance of ‘Redson’ Fruit: A New Red Pomelo x Grapefruit Hybrid
Authors: Salto Leanne , Maoz Itay , Goldenberg Livnat , Carmi Nir , Porat Ron
Published: October 2024

‘Redson’ is a new triploid, red-fleshed pomelo x grapefruit hybrid. The goal of this study was to examine the effects of rainfall, harvest time, tree age, and yield on the postharvest storage performance of ‘Redson’ fruit. During 2022/23, two postharvest storage trials were conducted with early- and late-harvested fruit. The fruit from the early harvest retained good quality for up to 16 weeks of storage at 7.5 °C plus 1 week at 22 °C, whereas the late-harvested fruit suffered from a high decay incidence. During 2023/24, we expanded the postharvest trials to nine different fruit sets harvested from early season (late October) until the end of the season (January). Fruit quality was examined under the same storage conditions after 6 and 16 weeks, and the results indicated that early- and mid-season fruit retained good quality with minimal decay incidence even after prolonged storage for 16 weeks, whereas the late-season fruit suffered from significant decay incidences of 17–22% and a decline in flavor acceptability. Further analysis revealed strong and significant correlations between various rainfall parameters and harvest time and decay incidences. Overall, early-harvested fruit during the autumn had a superior postharvest storage performance, whereas late-harvested fruit during the rainy winter suffered from decay development.
The Spatiotemporal Variations in and Propagation of Meteorological, Agricultural, and Groundwater Droughts in Henan Province, China
Authors: Xue Huazhu , Zhang Ruirui , Luan Wenfei , Yuan Zhanliang
Published: October 2024

As the global climate changes and droughts become more frequent, understanding the characteristics and propagation dynamics of drought is critical for monitoring and early warning. This study utilized the Standardized Precipitation Evapotranspiration Index (SPEI), Vegetation Condition Index (VCI), and Groundwater Drought Index (GDI) to identify meteorological drought (MD), agricultural drought (AD), and groundwater drought (GD), respectively. Sen’s slope method and Mann–Kendall trend analysis were used to examine drought trends. The Pearson correlation coefficient (PCC) and theory of run were utilized to identify the propagation times between different types of droughts. Cross-wavelet transform (XWT) and wavelet coherence (WTC) were applied to investigate the linkages among the three types of droughts. The results showed that, from 2004 to 2022, the average durations of MD, AD, and GD in Henan Province were 4.55, 8.70, and 29.03 months, respectively. MD and AD were gradually alleviated, while GD was exacerbated. The average propagation times for the different types of droughts were as follows: 6.1 months (MD-AD), 4.4 months (MD-GD), and 16.3 months (AD-GD). Drought propagation exhibited significant seasonality, being shorter in summer and autumn than in winter and spring, and there were close relationships among MD, AD, and GD. This study revealed the characteristics and propagation dynamics of different types of droughts in Henan Province, providing scientific references for alleviating regional droughts and promoting the sustainable development of agriculture and food production.
A Non-Destructive Measurement Approach for the Internal Temperature of Shiitake Mushroom Sticks Based on a Data–Physics Hybrid-Driven Model
Authors: Zhang Xin , Zeng Xinwen , Wei Yibo , Zheng Wengang , Wang Mingfei
Published: October 2024

This study aimed to develop a non-destructive measurement method utilizing acoustic sensors for the efficient determination of the internal temperature of shiitake mushroom sticks during the cultivation period. In this research, the sound speed, air temperature, and moisture content of the mushroom sticks were employed as model inputs, while the temperature of the mushroom sticks served as the model output. A data–physics hybrid-driven model for temperature measurement based on XGBoost was constructed by integrating monotonicity constraints between the temperature of the mushroom sticks and sound speed, along with the condition that limited the difference between air temperature and stick temperature to less than 2 °C. The experimental results indicated that the optimal eigenfrequency for applying this model was 850 Hz, the optimal distance between the sound source and the shiitake mushroom sticks was 8.7 cm, and the temperature measurement accuracy was highest when the moisture content of the shiitake mushroom sticks was in the range of 56~66%. Compared to purely data-driven models, our proposed model demonstrated significant improvements in performance; specifically, RMSE, MAE, and MAPE decreased by 74.86%, 77.22%, and 69.30%, respectively, while R2 increased by 1.86%. The introduction of physical knowledge constraints has notably enhanced key performance metrics in machine learning-based acoustic thermometry, facilitating efficient, accurate, rapid, and non-destructive measurements of internal temperatures in shiitake mushroom sticks.
Object Detection in High-Resolution UAV Aerial Remote Sensing Images of Blueberry Canopy Fruits
Authors: Zhao Yun , Li Yang , Xu Xing
Published: October 2024

Blueberries, as one of the more economically rewarding fruits in the fruit industry, play a significant role in fruit detection during their growing season, which is crucial for orchard farmers’ later harvesting and yield prediction. Due to the small size and dense growth of blueberry fruits, manual detection is both time-consuming and labor-intensive. We found that there are few studies utilizing drones for blueberry fruit detection. By employing UAV remote sensing technology and deep learning techniques for detection, substantial human, material, and financial resources can be saved. Therefore, this study collected and constructed a UAV remote sensing target detection dataset for blueberry canopy fruits in a real blueberry orchard environment, which can be used for research on remote sensing target detection of blueberries. To improve the detection accuracy of blueberry fruits, we proposed the PAC3 module, which incorporates location information encoding during the feature extraction process, allowing it to focus on the location information of the targets and thereby reducing the chances of missing blueberry fruits. We adopted a fast convolutional structure instead of the traditional convolutional structure, reducing the model’s parameter count and computational complexity. We proposed the PF-YOLO model and conducted experimental comparisons with several excellent models, achieving improvements in mAP of 5.5%, 6.8%, 2.5%, 2.1%, 5.7%, 2.9%, 1.5%, and 3.4% compared to Yolov5s, Yolov5l, Yolov5s-p6, Yolov5l-p6, Tph-Yolov5, Yolov8n, Yolov8s, and Yolov9c, respectively. We also introduced a non-maximal suppression algorithm, Cluster-NMF, which accelerates inference speed through matrix parallel computation and merges multiple high-quality target detection frames to generate an optimal detection frame, enhancing the efficiency of blueberry canopy fruit detection without compromising inference speed.
Dynamics of Energy Fluxes in a Mediterranean Vineyard: Influence of Soil Moisture
Authors: Egipto Ricardo , Aquino Arturo , Andújar José Manuel
Published: October 2024

Accurate evaluation of grapevine water use is essential for optimizing water management and maximizing grapevine yield and berry quality in Mediterranean climates. Understanding the water and heat flux dynamics in a vineyard during grapevine berry maturation is of utmost importance. This study focuses on evaluating sensible and latent energy fluxes at the canopy, the soil beneath the canopy, and the interrow areas. The primary objective is to develop a model framework for accurately estimating these energy fluxes, contributing to a better understanding of their behavior during berry ripening. The model’s accuracy was assessed by comparing the estimated fluxes with those measured by an eddy-covariance system installed at a reference height of three meters in the experimental vineyard. This validation step was essential to confirm the model’s ability to capture the intricate energy flux dynamics of the vineyard, especially during grape maturation. The results revealed a high level of agreement between the observed and estimated fluxes, confirming the model’s reliability. This comprehensive evaluation of energy fluxes provides valuable insights for optimizing irrigation strategies. By doing so, this study contributes to improving grape quality, ensuring sustainable water resource use, and ultimately enhancing vineyard productivity in arid and water-scarce regions.
Land Degradation in Southern Africa: Restoration Strategies, Grazing Management, and Livelihoods
Authors: Slayi Mhlangabezi , Zhou Leocadia , Thamaga Kgabo Humphrey
Published: October 2024

Land degradation in communal rangelands poses significant challenges to environmental sustainability, agricultural productivity, and livelihoods in southern Africa. This study presents a bibliometric analysis of research trends, key contributors, thematic evolution, and collaborative networks in the field of land degradation in communal rangelands from 1997 to 2024. Utilizing data obtained from the Scopus database, we examined 66 publications to identify patterns in publication output, leading journals, influential articles, and prominent authors and institutions. The analysis demonstrates an overall increase in research output, with a notable surge in publications during the past decade, indicating a growing academic and policy interest in this field. Major themes identified include sustainable land management, restoration strategies, and the impacts of grazing management on ecosystem health. Networks showcasing co-authorship and keyword co-occurrence reveal robust collaborative connections among researchers and a concentrated focus on specific dominant themes. Consequently, these findings propose opportunities for expanding interdisciplinary research and exploring underrepresented areas. This study provides a comprehensive overview of the research landscape, offering insights to steer future studies and inform policy interventions aimed at mitigating land degradation and bolstering the resilience of communal rangelands in southern Africa.
The Role of Fertilization on Soil Carbon Sequestration in Bibliometric Analysis
Authors: Zheng Han , Xu Yue , Wang Min , Qi Lin , Lian Zhenghua , Hu Lifang , Hu Hangwei , Ma Bin , Lv Xiaofei
Published: October 2024

The soil carbon pool is the largest and most dynamic carbon reservoir in terrestrial ecosystems. Fertilization, an important component of agricultural management, is a significant factor influencing soil carbon sequestration. This study analyzed literature from the Web of Science from 2008 to 2024 using CiteSpace. The results revealed a steady increase in publications on this topic, with a significant surge in the recent four years. The analysis highlighted key collaborations among countries, institutions, and authors, and identified main journal sources and seminal works in the research on the role of fertilization in soil carbon sequestrations. Keyword analysis indicated that current research hotspots include ‘soil organic carbon dynamics and organic matter decomposition’, ‘microbial community dynamics and carbon cycling’, and ‘agricultural management practices on carbon sequestration’. In the context of climate change, future research is likely to focus on enhancing sustainable agricultural practices, promoting biochar and resource utilization, and utilizing microbial communities to optimize soil carbon sequestration. This study provides a comprehensive overview of the role of fertilization in soil carbon sequestration, providing important insights for improving soil carbon sequestration strategies.
Evaluation of Kabuli Chickpea Genotypes for Tropical Adaptation in Northern Australia
Authors: Subedi Megha , Naiker Mani , du Preez Ryan , Adorada Dante L. , Bhattarai Surya
Published: October 2024

Chickpea is one of the economically important legume crops adapted for winter season production in tropical climates. This study evaluated the physiological, morphological, and biochemical traits of eight Kabuli chickpea genotypes in an Australian tropical environment. The result revealed significant differences between genotypes for seed emergence, plant height, primary shoots, leaf number, leaf area index, gas-exchange parameters, seed yield, carbon discrimination (Δ13C), and natural abundance for nitrogen fixation. Among the tested genotypes, AVTCPK#6 and AVTCPK#19 exhibited late flowering (60–66 days) and late maturity (105–107 days), and had higher leaf photosynthetic rate (Asat) (28.4–31.2 µmol m−2 s−1), lower stomatal conductance (gsw) (516–756 mmol m−2 s−1), were associated with reduced transpiration rate (T) (12.3–14.5 mmol m−2 s−1), offered greater intrinsic water-use efficiency (iWUE) (2.1–2.3 µmol m−2 s−1/mmol m−2 s−1), and contributed a higher seed yield (626–746 g/m2) compared to other genotypes. However, a larger seed test weight (>60 g/100 seed) was observed for AVTCPK#24, AVTCPK#8, and AVTCPK#3. Similarly, a high proportion (45%) of larger seeds (>10–11 mm) was recorded for AVTCPK#24. Furthermore, a higher %Ndfa in AVTCPK#6 (71%) followed by AVTCPK#19 (63%) indicated greater symbiotic nitrogen fixation in high-yielding genotypes. Positive correlation was observed between %Ndfa and seed protein, as well as between seed yield and plant height, primary shoots, leaf count, leaf area index, leaf photosynthesis, stomatal conductance, transpiration rate at pod filling stage, biomass, and harvest index. An inverse correlation between (Δ13C) and iWUE, particularly in AVTCPK#6 and AVTCPK#19, indicates greater heat and drought tolerance, required for high-yielding Kabuli chickpea production in northern Australia.
Ensiling of Willow and Poplar Biomass Is Improved by Ensiling Additives
Authors: Larsen Søren Ugilt , Hestbjerg Helle , Jørgensen Uffe , Kongsted Anne Grete
Published: October 2024

Biomass from willow and poplar harvested for feed during the growing season may be preserved by ensiling; however, little research has focused on ensiling of these biomasses. This study focuses on the use of ensiling additives to reduce the pH to around 4.0 to secure stable storage. Lab-scale ensiling experiments were conducted with different willow and poplar clones, shoot ages, and harvest times (June or September). Ensiling without additives often resulted in limited pH reduction. The pH could be reduced in the biomass of both species by adding formic acid, and the required dose to reduce the pH to 4.0 (buffering capacity, BC) ranged significantly between biomass types but was in the range of 2–5 kg formic acid (78%) per ton fresh weight. BC decreased with increasing dry matter (DM) content and decreasing crude protein content. The pH could also be reduced during ensiling by applying molasses and/or lactic acid bacteria, although not sufficiently in poplar. Willow biomass was ensiled effectively at the pilot scale with less than 7% DM loss by adding formic acid or by mixing with grass biomass. Comparable pH results were obtained at the lab scale and pilot scale. The study demonstrates how willow and poplar can be ensiled; however, more research is needed on quality changes during ensiling.
Analyses of Wheat Resistance to Fusarium Head Blight Using Different Inoculation Methods
Authors: Syed Shayan , Aleliūnas Andrius , Lillemo Morten , Gorash Andrii
Published: October 2024

Fusarium head blight is a devastating wheat disease that causes yield reduction and mycotoxins contamination, leading to multiple negative consequences for the economy, health, and food safety. Despite the tremendous efforts that have been undertaken over the last several decades to harness the disease, the problem remains a challenging issue. Due to global warming, its impact has become increasingly severe in Baltic and Nordic countries. The improvement of wheat resistance is hampered by complicated genetic inheritance, the scarcity of adapted resistant breeding materials, and difficulties in obtaining accurate and reproducible data due to the high interaction and dependency of the disease development on the environment. In this study, the resistance of 335 genotypes, 9 of which were of exotic origin and the remainder of which were adapted to the environments of Lithuania, Latvia, Estonia, or Norway, was studied in 8 trials using spray and point inoculation with spore suspensions and grain spawn inoculation under field and/or greenhouse conditions. The best linear unbiased estimates (BLUEs) of each genotype within the individual trials and the adjusted means across the trials were determined to reduce the environmental effects. Genotypes that exhibited excellent Type I or Type II resistance and overall resistance were identified.
Effectiveness of Diachasmimorpha longicaudata in Killing Ceratitis capitata Larvae Infesting Commercial Fruits in Dryland Agroecosystems of Western Argentina
Authors: Suárez Lorena del Carmen , Núñez-Campero Segundo Ricardo , Murúa Fernando , Garcia Flávio Roberto Mello , Ovruski Sergio Marcelo
Published: October 2024

Ceratitis capitata (Wiedemann) (medfly) strongly affects Argentinean fruit production and export. Augmentative biological control using the exotic parasitoid Diachasmimorpha longicaudata (Ashmead) is currently applied to this problem. The ability to find and parasitize medfly larvae on a wide diversity of fruit host species is a key issue that needs to be analyzed. This research assessed the effect of the physical features of fruit on the preference of foraging D. longicaudata females and the influence of varying release density on parasitoid performance as a pest mortality factor in three fruit species. Trials were performed inside field cages under semi-arid environmental conditions in Argentina’s central-western fruit-growing region. Sweet orange, peach, and fig were tested. The fruits were inoculated with third-instar larvae of the Vienna-8 temperature-sensitive lethal medfly strain. Naïve, 5 d-old mated D. longicaudata females were released in cages at 20, 40, 80, and 160 parasitoid densities. The highest levels of medfly mortality and parasitoid emergence were recorded in fig and peach, although D. longicaudata also induced mortality in orange, a fruit with few physical features favorable to parasitism. The medfly mortality in all fruit host species significantly increased with an increased number of parasitoid females released into the field cages. Diachsmimorpha longicaudata has high potential as a medfly biocontrol agent.
Management Practices in Mountain Meadows: Consequences for Soil Nutrient Availability
Authors: Jarne Adrián , Usón Asunción , Reiné Ramón
Published: October 2024

Soil nutrient availability in meadows has been poorly studied from the management point of view, despite its great impact. In this study, three different types of meadows have been analysed, as follows: intensive meadows, with high livestock load and inorganic fertilization; semi-extensive meadows, with medium livestock load and organic fertilization; and extensive meadows, with low livestock load and low fertilization rates. We looked at the nitrogen, phosphorus, potassium and carbon balances of each meadow type during two different years. Nitrogen was more stable in semi-extensive and extensive meadows, due to its organic form. In contrast, intensive meadows showed higher nitrogen variability depending on climate. Phosphorus is seen as the limiting nutrient, and it accumulates less in the soil than what is estimated in the crop balance, being more balanced in extensive meadows. Potassium has a strong response to temperature, being more available in June than in February, but crop balance was always negative for extensive meadows, and its soil concentration decreases each year, which could cause long-term potassium deficiency. Carbon accumulation was more stable in extensive meadows, where there was accumulation regardless of the year, whereas intensive and semi-extensive meadows become carbon emitters during the drought year.
Structural Derivatives of β-Asarone from Acorus calamus Linn. as Insecticide Candidates and the Insecticidal Mechanism Against Small Brown Planthopper
Authors: Wang Aiyu , Zhou Yun , Fu Xiaochen , Wang Xin , Cheng Yinjie , Zhang Yifei , Jia Xiuwen , Zhu Yanwei , Zhang Yun , Xue Chao , Shan Chenggang , Zhao Ming , Yang Yuanxue , Zhang Jianhua
Published: October 2024

The small brown planthopper (SBPH), Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae), is an increasing threat to Gramineae crops, posing significant risks to both the environment and food safety. β-asarone, as a promising green alternative to chemical insecticides, possesses wide application prospects in the crop protection field. To enhance the insecticidal activity of β-asarone, a series of derivatives were prepared through an active substructure splicing strategy, and their insecticidal activities against SBPH were evaluated. Among the 7 commercial compounds with chemical structures similar to β-asarone and 12 structural derivatives of β-asarone, compound 10, which incorporates the 2-chloropyridine functional group from flupyrimin, exhibited the most potent insecticidal activity against SBPH, with an 8.31-fold increase in insecticidal activity compared to β-asarone. Furthermore, transcriptome analysis showed that among the selected genes that may play important roles in insecticidal activity, an ABC transporter gene, MDR49, was most significantly down-regulated. MDR49 was highly expressed in the 4th-instar nymphs, with the highest expression level in the fat body, midgut, and abdomen. RNA interference (RNAi) against MDR49 significantly reduced susceptibility to compound 10 in SBPH, which revealed that MDR49 may be the candidate insecticidal target of compound 10. Additionally, the insecticidal spectrum revealed that compound 10 showed excellent efficacy against Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and Tetranychus cinnabarinus (Boisduval) (Acarina: Tetranychidae). This study indicates that compound 10 could be further developed as a novel eco-friendly pesticide.
WRKY Transcription Factors (TFs) as Key Regulators of Plant Resilience to Environmental Stresses: Current Perspective
Authors: Li Shenglin , Khoso Muneer Ahmed , Xu He , Zhang Chao , Liu Ziyang , Wagan Sindho , Dinislam Khuzin , Liu Lijie
Published: October 2024

Plants encounter various stresses in their natural environments and can effectively respond to only one stress at a time. Through a complex gene network, transcription factors (TFs) such as WRKY TFs regulate a diverse array of stress responses. The clarification of the structural characteristics of WRKY proteins, along with recent advancements in molecular dynamics simulations, has shed light on the formation, stability, and interactions of DNA–protein complexes. This has provided a novel viewpoint regarding the control of WRKY TFs. The investigation of superfamilies, encompassing their historical development, diversity, and evolutionary patterns, has become feasible due to the transcriptome approach’s capacity to provide extensive and comprehensive transcripts. The significance of WRKY TFs lies in their pivotal role within several signaling cascades and regulatory networks that influence plant defense responses. The present review summarizes the functional aspects of the high-volume sequence data of WRKY TFs from different species studied to date. Moreover, a comparative analysis approach was utilized to determine the functions of the identified WRKY TFs in response to both abiotic and biotic stresses, as revealed through numerous studies on different plant species. The results of this review will be pivotal in understanding evolutionary events and the significance of WRKY TFs in the context of climate change, incorporating new scientific evidence to propose an innovative viewpoint.
Cotton Response to Foliar Potassium Application in South Texas Dryland
Authors: Kommineni Varshith , Bhandari Ammar B. , Schuster Greta , Nelson Shad D.
Published: October 2024

Potassium (K) deficiency is common in cotton (Gossypium hirsutum L.)-growing areas. This study aims to investigate the effects of different rates of foliar K fertilizer application on three cotton varieties: NG 5711 B3XF (V1), PHY 480 W3FE (V2), and FM 1953GLTP (V3). Potassium fertilizer was dissolved in water and was foliar-applied at 34, 50, and 67 kg ha−1. Cotton plant height (CH) and canopy width (CW) were monitored throughout the growing season. The results showed that foliar K fertilizer application significantly impacted the CH and CW in dry years. Although insignificant, the cotton lint yield increased by 15% and 20% with 34 and 50 kg ha−1 in 2020 and by 9% and 7% with 50 and 67 kg ha−1 in 2021, indicating the potential for improved lint yield with foliar K application in rainfed production systems. Similarly, variety V3 had significantly greater lint and seed yields than V1 in 2020. The average lint yield among the varieties was 32%, and the seed yield was 27% greater in 2020 than in 2021. The cotton fiber color grade was significantly greater at 50 kg ha−1 in 2020 and 67 kg ha−1 in 2021. Cotton variety significantly affected color grade, uniformity, staple length, Col, RD, and Col-b contents in 2020 and 2021. The results suggest that foliar K application can enhance cotton production in rainfed production systems. However, more research is required to quantify varietal and foliar K application rates for improved lint yield and quality.
Warming Increases Ecological Niche of Leymus chinensis but Is Detrimental to Species Diversity in Inner Mongolia Temperate Grasslands
Authors: Zhang Xingbo , Wan Zhiqiang , Gu Rui , Dong Lingman , Chen Xuemeng , Chun Xi , Zhou Haijun , Zhang Weiqing
Published: October 2024

Dominant species are crucial in regulating the structure and productivity of plant communities. Adaptation strategies to climate change vary among the dominant species of different life types. However, the responses of the ecological niches of dominant species to warming and precipitation in semi-arid grasslands and their impacts on community structure and function are unknown. This study involved conducting a long-term experimental simulation of warming and increased precipitation on grasslands in Inner Mongolia and studying population dynamics, ecological niches, and their responses to the structure and function of the community species of two dominant plants, L. chinensis (perennial rhizome grass) and S. krylovii (perennial clumped grass). The results show that the niche width of L. chinensis increased and S. krylovii decreased under warming and increased precipitation conditions. The overlap of L. chinensis and S. krylovii decreased under the same conditions. The niche widths of L. chinensis and S. krylovii were 1.22 for the control (C), 1.19 and 1.04 under warming (W) conditions, 1.27 and 0.97 under warming plus precipitation (WP) conditions, and 1.27 and 1.24 under the conditions of precipitation addition (P). The niche overlap of L. chinensis and S. krylovii were 0.72 in C, 0.69 in W, 0.68 in WP, and 0.82 in P. The biomass share and importance value of L. chinensis increased, and those of S. krylovii decreased in response to warming and precipitation. The effects of warming on species diversity and community stability are primarily influenced by the effects on the niche breadth of S. krylovii. Combined with our previous study, L. chinensis will offer more resources in communities in warmer and wetter steppe climates in the future. However, this is not conducive to community diversity.
Characterization and Greenhouse Trial of Zn Bio-Chelates Derived from Spent Coffee Grounds
Authors: Cervera-Mata Ana , Lara-Ramos Leslie , Rufián-Henares José Ángel , Fernández-Arteaga Alejandro , Fernández-Bayo Jesús , Delgado Gabriel
Published: October 2024

The conversion of spent coffee grounds (SCG) into hydrochars has been the subject of extensive research in recent years, aimed at evaluating their potential for biofortifying foods and mitigating the plant toxicity linked to SCG. This study aimed to assess the physicochemical characterization and the impact of incorporating both activated (ASCG and AH160) and functionalized SCG (ASCG-Zn), as well as SCG-derived hydrochars (AH160-Zn), on cucumber yield and plant zinc content. The following physicochemical properties were analyzed: specific surface area, pH and electrical conductivity, polyphenols, and nuclear magnetic resonance. The by-products activated and functionalized with zinc were applied to cucumber crops grown in a greenhouse across multiple harvests. The activation of both SCG and H160 reduced the specific surface area of the particles. However, when these by-products were functionalized, their Zn content increased significantly, up to 7400 ppm. Concerning polyphenol content, the activated products showed levels ranging from 3.5 to 4.9 mg GAE/g. Regarding cumulative production, the treatments that showed the highest yields were the by-products activated and functionalized with Zn reaching 25 kg. Incorporating these by-products notably raised the Zn content in cucumbers, reaching 0.1 mg Zn per 100 g of fresh weight. The activated by-products demonstrated the highest Zn utilization efficiency.
Effect of Different Fertilization Strategies on Infestation of Brown Wheat Mite and Wheat Productivity
Authors: Kalmosh Fatma Sh. , Ibrahim M. M. A. , Lv Jiale , Saleh Ibrahim A. , Al-Hawadie Jehad S. , Al-Qahtani Wahidah H.
Published: October 2024

The brown wheat mite, Petrobia tritici, poses a significant threat to wheat fields. While fertilizers can increase crop productivity, imbalanced application may exacerbate plant susceptibility to pests. This study aimed to evaluate the impact of various NPK fertilization programs on P. tritici infestations over two consecutive cropping seasons. The results revealed significant differences in mite infestation among the treatment groups (p < 0.001). The lowest populations (1.1 and 3.0 mites/leaf) were observed in the treatments sprayed with phosphoric acid (at 0.75 and 1.00 cm/L), where the infestation appeared approximately 120 days after sowing; in contrast, it appeared early at 75 days in the other treatments. Conversely, treatments lacking potassium fertilizer presented the greatest degree of mite injury levels (49.5–57.7 mites/leaf). Although these treatments provided moderate leaf nutrition and crop yield, the highest nutritional content and total yield (10.49 and 9.71 1 t/ha for the two years) were observed in the treatment that received 224:70:100 kg fad−1 commercial fertilizers (=178:25:114 kg ha−1 NPK units) as soil fertilization, which was followed by the treatment with a foliar application of phosphoric acid (1.00 cm/L) with a total yield of 9.34 and 8.53 1 t/ha for the two years. In this treatment, the P. tritici density was moderately high at 9.40 and 6.32 mites/leaf over the two years, respectively. The consistency of P. tritici density and total yield ranking across both years indicated reliable estimates of the impact of fertilization. This study suggests that potassium sulfate application is crucial for reducing P. tritici density and that foliar phosphoric acid application instead of soil application reduces the number of P. tritici and delays its occurrence.
Assessment of the Effects of Biochar on the Physicochemical Properties of Saline–Alkali Soil Based on Meta-Analysis
Authors: Mao Tingting , Wang Yaofeng , Ning Songrui , Mao Jiefei , Sheng Jiandong , Jiang Pingan
Published: October 2024

Enhancing global agricultural sustainability critically requires improving the physicochemical properties of saline–alkali soil. Biochar has gained increasing attention as a strategy due to its unique properties. However, its effect on the physicochemical properties of saline–alkali soil varies significantly. This study uses psychometric meta-analysis across 137 studies to synthesize the findings from 1447 relatively independent data sets. This study investigates the effects of biochar with different characteristics on the top 20 cm of various saline–alkali soils. In addition, aggregated boosted tree (ABT) analysis was used to identify the key factors of biochar influencing the physicochemical properties of saline soils. The results showed that biochar application has a positive effect on improving soil properties by reducing the sodium adsorption ratio (SAR) and the exchangeable sodium percentage (ESP) by 30.31% and 28.88%, respectively, with a notable 48.97% enhancement in cation exchange capacity (CEC). A significant inverse relationship was found between soil salinity (SC) and ESP, while other factors were synergistic. Biochar application to mildly saline soil (<0.2%) and moderately saline soil (0.2–0.4%) demonstrated greater improvement in soil bulk density (SBD), total porosity (TP), and soil moisture content (SMC) compared to highly saline soil (>0.4%). However, the reduction in SC in highly saline soil was 4.9 times greater than in moderately saline soils. The enhancement of soil physical properties positively correlated with higher biochar application rates, largely driven by soil movements associated with the migration of soil moisture. Biochar produced at 401–500 °C was generally the most effective in improving the physicochemical properties of various saline–alkali soils. In water surplus regions, for mildly saline soil with pH < 8.5, mixed biochar (pH 6–8) at 41–80 t ha−1 was the most effective in soil improvement. Moreover, in water deficit areas with soil at pH ≥ 8.5, biochar with pH ≤ 6 applied at rates of >80 t ha−1 showed the greatest benefits. Agricultural residue biochar showed superior efficiency in ameliorating highly alkaline (pH ≥ 8.5) soil. In contrast, the use of mixed types of biochar was the most effective in the amelioration of other soil types.
Analysis of the Physiological Parameters of Cold Resistance in Core Winter and Spring Wheat Cultivars
Authors: Wang Yunhe , Bo Cunyao , Wang Xiaohua , Yang Xincheng , Wang Hongwei
Published: October 2024

We selected 46 core winter–spring wheat cultivars from China’s main wheat-producing areas as experimental materials to clarify the differences in the physiological parameters of their cold resistance and provide a theoretical basis and high-quality germplasm for cold resistance breeding. We investigated differences in their cold resistance under field conditions for two consecutive years, and determined the physiological parameters of the cold resistance, yield, and quality indicators of different winter–spring wheat cultivars. Our results showed that the cold resistance of winter wheat cultivars was higher than that of spring wheat cultivars. The chlorophyll (Chl), soluble sugar (SS), soluble protein (SP), and free proline (Pro) contents of different winter–spring wheat cultivars were positively correlated with cold resistance, and malondialdehyde (MDA) content was negatively correlated with cold resistance. The five physiological parameters can be used as physiological indicators for the breeding of cold-resistant cultivars. The cold resistance, yield, and quality indicators of different spring and winter wheat cultivars were comprehensively evaluated by using the average membership value and comprehensive score. It was found that the average membership value and comprehensive score of winter wheat cultivars were higher than those of spring wheat cultivars. Through classification using the K-means method, the cold-resistant, high-yield, and high-quality cultivars were screened out, namely, Jimai23 (JM23), Jimai44 (JM44), Shannong57 (SN57), and Jinmai 919 (JM919).