bluuebunny/crossref_metadata_2025 huggingface dataset:
Landscape Grain Effect in Yancheng Coastal Wetland and Its Response to Landscape Changes
Authors: Tian Peng , Cao Luodan , Li Jialin , Pu Ruiliang , Shi Xiaoli , Wang Lijia , Liu Ruiqing , Xu Hao , Tong Chen , Zhou Zijing , Shao Shuyao
Published: June 2019

The landscape grain effect reflects the spatial heterogeneity of a landscape and it is used as a research core of landscape ecology. The landscape grain effect can be used to not only explore spatiotemporal variation characteristics of a landscape pattern, but also to disclose variation laws of ecological structures and functions of landscapes. In this study, the sensitivity of landscape pattern indexes to grain sizes 50–1000 m was studied based on landscape data in Yancheng Coastal Wetland acquired in 1991, 2000, 2008, and 2017. Response of the grain effect to landscape changes was analyzed and an optimal grain size for analysis in the study area was determined. Results indicated that: (1) among 27 indexes (12 in a class level and 15 in a landscape level), eight indexes were highly sensitive to grains, ten indexes presented moderate sensitivity, eight indexes presented low sensitivity, and one was unresponsive. It was shown that the area-margin index and the shape index were more sensitive to the different grain sizes. The aggregation index had some differences in the grain size change, and the diversity index had a low response degree to the grain size. (2) Landscape indexes showed six different responses to different grains, including slow reduced response, fast reduced and then slow reduced response, monotonically increased response, fluctuating reduced response, up-down responses, and stable response, which indicated that the landscape index was closely related to the spatial grain. (3) From 1991 to 2017, variation curves of the landscape grain size of different landscape types could be divided into four types: fluctuation rising type, fluctuation type, monotonous decreasing type, and monotonous rising type. Different grain size curves had different interpretations of landscape changes, but in general, Yancheng Coastal Wetland’s landscape tended to be fragmented and complicated, internal connectivity was weakened, and dominant landscape area was reduced. Natural wetlands were more sensitive to grain size effects than artificial wetlands. (4) The landscape index at the 50 m grain size had a strong response to different grain size changes, and the loss of landscape information was the smallest. Therefore, it was determined that the optimal landscape grain size in the study area was 50 m.
Spatio-Temporal Characterization Analysis and Water Quality Assessment of the South-to-North Water Diversion Project of China
Authors: Nong Xizhi , Shao Dongguo , Xiao Yi , Zhong Hua
Published: June 2019

In this article, a data matrix of 20 indicators (6960 observations) was obtained from 29 water quality monitoring stations of the Middle Route (MR) of the South-to-North Water Diversion Project of China (SNWDPC). Multivariate statistical techniques including analysis of variance (ANOVA), correlation analysis (CA), and principal component analysis (PCA) were applied to understand and identify the interrelationships between the different indicators and the most contributive sources of anthropogenic and natural impacts on water quality. The water quality index (WQI) was used to assess the classification and variation of water quality. The distributions of the indicators revealed that six heavy-metal indicators including arsenic (As), mercury (Hg), cadmium (Cd), chromium (Cr), selenium (Se), and lead (Pb) were within the Class I standard, while the As, Pb, and Cd displayed spatial variation. Moreover, some physicochemical indicators such as dissolved oxygen, 5-day biochemical oxygen demand (as BOD5), and total phosphorus (TP) had spatio-temporal variability. The correlation analysis result demonstrated that As, Hg, Cd, Cr, Se, Pb, copper (Cu), and zinc (Zn) had high correlation coefficients. The PCA result extracted three principal components (PC) accounting for 82.67% of the total variance, while the first PC was indicative of the mixed sources of anthropogenic and natural contributions, the second and the third PCs were mainly controlled by human activities and natural sources, respectively. The calculation results of the WQI showed an excellent water quality of the MR of the SNWDPC where the values of the stations ranged from 10.49 to 17.93, while Hg was the key indicator to determine the WQI > 20 of six stations, which indicated that the Hg can be the main potential threat to water quality and human health in this project. The result suggests that special attention should be paid to the monitoring of Hg, and the investigation and supervision within the areas of high-density human activities in this project should be taken to control the impacts of urban and industrial production and risk sources on water quality.
Whole Genome Sequencing and Analysis of Chlorimuron-Ethyl Degrading Bacteria Klebsiella pneumoniae 2N3
Authors: Zhang Cheng , Hao Qingkai , Zhang Zhengyi , Zhang Xianghui , Pan Hongyu , Zhang Jiahuan , Zhang Hao , Sun Fengjie
Published: June 2019

Klebsiella pneumoniae 2N3 is a strain of gram-negative bacteria that can degrade chlorimuron-ethyl and grow with chlorimuron-ethyl as the sole nitrogen source. The complete genome of Klebsiella pneumoniae 2N3 was sequenced using third generation high-throughput DNA sequencing technology. The genomic size of strain 2N3 was 5.32 Mb with a GC content of 57.33% and a total of 5156 coding genes and 112 non-coding RNAs predicted. Two hydrolases expressed by open reading frames (ORFs) 0934 and 0492 were predicted and experimentally confirmed by gene knockout to be involved in the degradation of chlorimuron-ethyl. Strains of ΔORF 0934, ΔORF 0492, and wild type (WT) reached their highest growth rates after 8–10 hours in incubation. The degradation rates of chlorimuron-ethyl by both ΔORF 0934 and ΔORF 0492 decreased in comparison to the WT during the first 8 hours in culture by 25.60% and 24.74%, respectively, while strains ΔORF 0934, ΔORF 0492, and the WT reached the highest degradation rates of chlorimuron-ethyl in 36 hours of 74.56%, 90.53%, and 95.06%, respectively. This study provides scientific evidence to support the application of Klebsiella pneumoniae 2N3 in bioremediation to control environmental pollution.
Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin
Authors: Shin Eun Ju , Jo Seongin , Choi Hyo-kyoung , Choi Sungbin , Byun Sanguine , Lim Tae-Gyu
Published: June 2019

Caffeic acid phenethyl ester (CAPE), a naturally occurring bioactive compound, displays anti-inflammatory, anti-carcinogenic, and anti-microbial effects. However, the effect of CAPE on skin photoaging is unknown. Herein, we investigated the inhibitory effect of CAPE against ultraviolet (UV) irradiation-mediated matrix metalloproteinase (MMP)-1 expression and its underlying molecular mechanism. CAPE treatment suppressed UV-induced MMP-1 levels in both human dermal fibroblasts (HDF) and human skin tissues. While CAPE did not display any significant effects against the upstream regulatory pathways of MMP-1, CAPE was capable of reversing UV-mediated epigenetic modifications. CAPE suppressed UV-induced acetyl-histone H3 (Lys9) as well as total lysine acetylation in HDF cells. Similarly, CAPE also attenuated UV-induced lysine acetylations in human skin tissues, suggesting that the CAPE-mediated epigenetic alterations can be recapitulated in ex vivo conditions. CAPE was found to attenuate UV-induced histone acetyltransferase (HAT) activity in HDF. Notably, CAPE was able to directly inhibit the activity of several HATs including p300, CREP-binding protein (CBP), and p300/CBP-associated factor (PCAF), further confirming that CAPE can function as an epigenetic modulator. Thus, our study suggests that CAPE maybe a promising agent for the prevention of skin photoaging via targeting HATs.
The Potential of Flavonoids for the Treatment of Neurodegenerative Diseases
Authors: Maher Pamela
Published: June 2019

Neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and amyotrophic lateral sclerosis (ALS), currently affect more than 6 million people in the United States. Unfortunately, there are no treatments that slow or prevent disease development and progression. Regardless of the underlying cause of the disorder, age is the strongest risk factor for developing these maladies, suggesting that changes that occur in the aging brain put it at increased risk for neurodegenerative disease development. Moreover, since there are a number of different changes that occur in the aging brain, it is unlikely that targeting a single change is going to be effective for disease treatment. Thus, compounds that have multiple biological activities that can impact the various age-associated changes in the brain that contribute to neurodegenerative disease development and progression are needed. The plant-derived flavonoids have a wide range of activities that could make them particularly effective for blocking the age-associated toxicity pathways associated with neurodegenerative diseases. In this review, the evidence for beneficial effects of multiple flavonoids in models of AD, PD, HD, and ALS is presented and common mechanisms of action are identified. Overall, the preclinical data strongly support further investigation of specific flavonoids for the treatment of neurodegenerative diseases.
Lipoprotein Lipase Inhibitor, Nordihydroguaiaretic Acid, Aggravates Metabolic Phenotypes and Alters HDL Particle Size in the Western Diet-Fed db/db Mice
Authors: Kang Inhae , Park Miyoung , Yang Soo Jin , Lee Myoungsook
Published: June 2019

Lipoprotein lipase (LPL) hydrolyzes triglycerides in lipoprotein to supply fatty acids, and its deficiency leads to hypertriglyceridemia, thereby inducing metabolic syndrome (MetSyn). Nordihydroguaiaretic acid (NDGA) has been recently reported to inhibit LPL secretion by endoplasmic reticulum (ER)-Golgi redistribution. However, the role of NDGA on dyslipidemia and MetSyn remains unclear. To address this question, leptin receptor knock out (KO)-db/db mice were randomly assigned to three different groups: A normal AIN76-A diet (CON), a Western diet (WD) and a Western diet with 0.1% NDGA and an LPL inhibitor, (WD+NDGA). All mice were fed for 12 weeks. The LPL inhibition by NDGA was confirmed by measuring the systemic LPL mass and adipose LPL gene expression. We investigated whether the LPL inhibition by NDGA alters the metabolic phenotypes. NDGA led to hyperglycemia, hypertriglyceridemia, and hypercholesterolemia. More strikingly, the supplementation of NDGA increased the percentage of high density lipoprotein (HDL)small (HDL3a+3b+3c) and decreased the percentage of HDLlarge (HDL2a+2b) compared to the WD group, which indicates that LPL inhibition modulates HDL subclasses. was NDGA increased adipose inflammation but had no impact on hepatic stress signals. Taken together, these findings demonstrated that LPL inhibition by NDGA aggravates metabolic parameters and alters HDL particle size.
Pulmonary Arterial Hypertension Due to NPR-C Mutation: A Novel Paradigm for Normal and Pathologic Remodeling?
Authors: Egom Emmanuel Eroume-A
Published: June 2019

Idiopathic Pulmonary Arterial Hypertension (IPAH) is a deadly and disabling disease characterized by severe vascular remodeling of small pulmonary vessels by fibroblasts, myofibroblasts and vascular smooth muscle cell proliferation. Recent studies suggest that the Natriuretic Peptide Clearance Receptor (NPR-C) signaling pathways may play a crucial role in the development of IPAH. Reduced expression or function of NPR-C signaling in pulmonary artery smooth muscle cells may contribute to the pulmonary vascular remodeling, which is characteristic of this disease. The likely mechanisms may involve an impaired interaction between NPR-C, specific growth factors and other signal transduction pathways including but not limited to Gqα/mitogen-activated protein kinase (MAPK)/PI3K and AKT signaling. The resulting failure of growth suppression in pulmonary artery smooth muscle cells provides critical clues to the cellular pathobiology of IPAH. The reciprocal regulation of NPR-C signaling in models of tissue remodeling may thus provide new insights to our understanding of IPAH.
ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense
Authors: Rustgi Sachin , Springer Armin , Kang ChulHee , von Wettstein Diter , Reinbothe Christiane , Reinbothe Steffen , Pollmann Stephan
Published: June 2019

The channeling of metabolites is an essential step of metabolic regulation in all living organisms. Multifunctional enzymes with defined domains for metabolite compartmentalization are rare, but in many cases, larger assemblies forming multimeric protein complexes operate in defined metabolic shunts. In Arabidopsis thaliana, a multimeric complex was discovered that contains a 13-lipoxygenase and allene oxide synthase (AOS) as well as allene oxide cyclase. All three plant enzymes are localized in chloroplasts, contributing to the biosynthesis of jasmonic acid (JA). JA and its derivatives act as ubiquitous plant defense regulators in responses to both biotic and abiotic stresses. AOS belongs to the superfamily of cytochrome P450 enzymes and is named CYP74A. Another CYP450 in chloroplasts, hydroperoxide lyase (HPL, CYP74B), competes with AOS for the common substrate. The products of the HPL reaction are green leaf volatiles that are involved in the deterrence of insect pests. Both enzymes represent non-canonical CYP450 family members, as they do not depend on O2 and NADPH-dependent CYP450 reductase activities. AOS and HPL activities are crucial for plants to respond to different biotic foes. In this mini-review, we aim to summarize how plants make use of the LOX2–AOS–AOC2 complex in chloroplasts to boost JA biosynthesis over volatile production and how this situation may change in plant communities during mass ingestion by insect pests.
Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland
Authors: Ventura Spagnolo Elvira , Mondello Cristina , Cardia Luigi , Minutoli Letteria , Puzzolo Domenico , Asmundo Alessio , Macaione Vincenzo , Alibrandi Angela , Malta Consuelo , Baldino Gennaro , Micali Antonio
Published: June 2019

The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. In conclusion, the authors provide a possible explanation for the observed differences in expression in relation to the cause of death.
FSH/LH-Dependent Upregulation of Ahr in Murine Granulosa Cells Is Controlled by PKA Signaling and Involves Epigenetic Regulation
Authors: Matvere Antti , Teino Indrek , Varik Inge , Kuuse Sulev , Tiido Tarmo , Kristjuhan Arnold , Maimets Toivo
Published: June 2019

The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor primarily known for its toxicological functions. Recent studies have established its importance in many physiological processes including female reproduction, although there is limited data about the precise mechanisms how Ahr itself is regulated during ovarian follicle maturation. This study describes the expression of Ahr in ovarian granulosa cells (GCs) of immature mice in a gonadotropin-dependent manner. We show that Ahr upregulation in vivo requires both follicle stimulating hormone (FSH) and luteinizing hormone (LH) activities. FSH alone increased Ahr mRNA, but had no effect on Ahr protein level, implicating a possible LH-dependent post-transcriptional regulation. Also, the increase in Ahr protein is specific to large antral follicles in induced follicle maturation. We show that Ahr expression in GCs of mid-phase follicular maturation is downregulated by protein kinase A (PKA) signaling and activation of Ahr promoter is regulated by chromatin remodeling.
Bioactive Peptide VHVV Upregulates the Long-Term Memory-Related Biomarkers in Adult Spontaneously Hypertensive Rats
Authors: Ju Da-Tong , K. Ashok Kumar , Kuo Wei-Wen , Ho Tsung-Jung , Chang Ruey-Lin , Lin Wan-Teng , Day Cecilia Hsuan , Viswanadha V. Vijaya Padma , Liao Po-Hsiang , Huang Chih-Yang
Published: June 2019

Hypertension is one of the growing risk factors for the progression of long-term memory loss. Hypertension-mediated memory loss and treatment remain not thoroughly elucidated to date. Plant-based natural compounds are an alternative solution to treating human diseases without side effects associated with commercial drugs. This study reveals that bioactive peptides extracted from soy hydrolysates mimic hypertension-mediated memory loss and neuronal degeneration and alters the memory molecular pathway in spontaneously hypertensive rats (SHR). The SHR animal model was treated with bioactive peptide VHVV (10 mg/kg/oral administration) and angiotensin-converting-enzyme (ACE) inhibitors (5 mg/kg/oral administration) for 24 weeks. We evaluated molecular level expression of brain-derived neurotrophic factor (BDNF), cAMP response element binding protein (CREB), and survival markers phospho-protein kinase B (P-AKT) and phosphoinositide 3-kinase (PI3K) after 24 weeks of treatment for SHR in this study. Western blotting, hematoxylin and eosin (H&E) staining, and immunohistochemistry showed long-term memory loss and neuronal degeneration in SHR animals. Bioactive peptide VHVV-treated animals upregulated the expression of long-term memory-relate proteins and neuronal survival. Spontaneously hypertensive rats treated with oral administration of bioactive peptide VHVV had activated CREB-mediated downstream proteins which may reduce hypertension-mediated long-term memory loss and maintain neuronal survival.
Anti-Obesity Effects of Dietary Calcium: The Evidence and Possible Mechanisms
Authors: Zhang Fenglin , Ye Jingjing , Zhu Xiaotong , Wang Lina , Gao Ping , Shu Gang , Jiang Qingyan , Wang Songbo
Published: June 2019

Obesity is a serious health challenge worldwide and is associated with various comorbidities, including dyslipidemia, type 2 diabetes, and cardiovascular disease. Developing effective strategies to prevent obesity is therefore of paramount importance. One potential strategy to reduce obesity is to consume calcium, which has been implicated to be involved in reducing body weight/fat. In this review, we compile the evidence for the anti-obesity roles of calcium in cells, animals, and humans. In addition, we summarize the possible anti-obesity mechanisms of calcium, including regulation of (a) adipogenesis, (b) fat metabolism, (c) adipocyte (precursor) proliferation and apoptosis, (d) thermogenesis, (e) fat absorption and excretion, and (f) gut microbiota. Although the exact anti-obesity roles of calcium in different subjects and how calcium induces the proposed anti-obesity mechanisms need to be further investigated, the current evidence demonstrates the anti-obesity effects of calcium and suggests the potential application of dietary calcium for prevention of obesity.
Nuclear-Biased DUSP6 Expression is Associated with Cancer Spreading Including Brain Metastasis in Triple-Negative Breast Cancer
Authors: Wu Fan , McCuaig Robert D. , Sutton Christopher R. , Tan Abel H. Y. , Jeelall Yoshni , Bean Elaine G. , Dai Jin , Prasanna Thiru , Batham Jacob , Malik Laeeq , Yip Desmond , Dahlstrom Jane E. , Rao Sudha
Published: June 2019

DUSP6 is a dual-specificity phosphatase (DUSP) involved in breast cancer progression, recurrence, and metastasis. DUSP6 is predominantly cytoplasmic in HER2+ primary breast cancer cells, but the expression and subcellular localization of DUSPs, especially DUSP6, in HER2-positive circulating tumor cells (CTCs) is unknown. Here we used the DEPArray system to identify and isolate CTCs from metastatic triple negative breast cancer (TNBC) patients and performed single-cell NanoString analysis to quantify cancer pathway gene expression in HER2-positive and HER2-negative CTC populations. All TNBC patients contained HER2-positive CTCs. HER2-positive CTCs were associated with increased ERK1/ERK2 expression, which are direct DUSP6 targets. DUSP6 protein expression was predominantly nuclear in breast CTCs and the brain metastases but not pleura or lung metastases of TNBC patients. Therefore, nuclear DUSP6 may play a role in the association with cancer spreading in TNBC patients, including brain metastasis.
Applications of Ultrasound to Stimulate Therapeutic Revascularization
Authors: Gorick Catherine M. , Chappell John C. , Price Richard J.
Published: June 2019

Many pathological conditions are characterized or caused by the presence of an insufficient or aberrant local vasculature. Thus, therapeutic approaches aimed at modulating the caliber and/or density of the vasculature by controlling angiogenesis and arteriogenesis have been under development for many years. As our understanding of the underlying cellular and molecular mechanisms of these vascular growth processes continues to grow, so too do the available targets for therapeutic intervention. Nonetheless, the tools needed to implement such therapies have often had inherent weaknesses (i.e., invasiveness, expense, poor targeting, and control) that preclude successful outcomes. Approximately 20 years ago, the potential for using ultrasound as a new tool for therapeutically manipulating angiogenesis and arteriogenesis began to emerge. Indeed, the ability of ultrasound, especially when used in combination with contrast agent microbubbles, to mechanically manipulate the microvasculature has opened several doors for exploration. In turn, multiple studies on the influence of ultrasound-mediated bioeffects on vascular growth and the use of ultrasound for the targeted stimulation of blood vessel growth via drug and gene delivery have been performed and published over the years. In this review article, we first discuss the basic principles of therapeutic ultrasound for stimulating angiogenesis and arteriogenesis. We then follow this with a comprehensive cataloging of studies that have used ultrasound for stimulating revascularization to date. Finally, we offer a brief perspective on the future of such approaches, in the context of both further research development and possible clinical translation.
Protein Expression Analysis in Reversible Photobleached Cells of Scenedesmus vacuolatus after High Temperature Stress
Authors: Ko Tzu-Hsing , Leu Kuen-Lin , Hsu Ban-Dar , Lee Tzan-Chain
Published: June 2019

We have analyzed protein expression in the bleached small vegetative cells of synchronous Scenedesmus vacuolatus to investigate how unicellular algae lived through stress. These cells were subjected to heat treatment (46.5 °C for 1h in dark condition) and then cultured under continuous illumination for 24 h. Flow cytometry analysis of the chlorophyll autofluorescence intensity of S. vacuolatus cells indicated that heat-treated cells were completely bleached within 24 h of light cultivation. Transmission electron microscopy (TEM) images showed that bleached cells maintained thylakoid membrane structure, but with lower contrast. The bleached cells regained green color after 72 h, along with a recovery in contrast, which indicated a return of photosynthetic ability. Two-dimensional gel electrophoresis (2DE) showed that the protein expression patterns were very difference between control and bleached cells. ATP synthase subunits and glutamine synthetase were down-regulated among the many differences, while some of phototransduction, stress response proteins were up-regulated in bleached cells, elucidating bleached cells can undergo changes in their biochemical activity, and activate some stress response proteins to survive the heat stress and then revive. In addition, small heat shock proteins (HSPs), but not HSP40 and HSP70 family proteins, protected the bleaching cells.
Proteolytic Rafts for Improving Intraparenchymal Migration of Minimally Invasively Administered Hydrogel-Embedded Stem Cells
Authors: Piejko Marcin , Jablonska Anna , Walczak Piotr , Janowski Miroslaw
Published: June 2019

The physiological spaces (lateral ventricles, intrathecal space) or pathological cavities (stroke lesion, syringomyelia) may serve as an attractive gateway for minimally invasive deployment of stem cells. Embedding stem cells in injectable scaffolds is essential when transplanting into the body cavities as they secure favorable microenvironment and keep cells localized, thereby preventing sedimentation. However, the limited migration of transplanted cells from scaffold to the host tissue is still a major obstacle, which prevents this approach from wider implementation for the rapidly growing field of regenerative medicine. Hyaluronan, a naturally occurring polymer, is frequently used as a basis of injectable scaffolds. We hypothesized that supplementation of hyaluronan with activated proteolytic enzymes could be a viable approach for dissolving the connective tissue barrier on the interface between the scaffold and the host, such as pia mater or scar tissue, thus demarcating lesion cavity. In a proof-of-concept study, we have found that collagenase and trypsin immobilized in hyaluronan-based hydrogel retain 60% and 28% of their proteolytic activity compared to their non-immobilized forms, respectively. We have also shown that immobilized enzymes do not have a negative effect on the viability of stem cells (glial progenitors and mesenchymal stem cells) in vitro. In conclusion, proteolytic rafts composed of hyaluronan-based hydrogels and immobilized enzymes may be an attractive strategy to facilitate migration of stem cells from injectable scaffolds into the parenchyma of surrounding tissue.
Drowsiness Estimation Using Electroencephalogram and Recurrent Support Vector Regression
Authors: Akbar Izzat Aulia , Igasaki Tomohiko
Published: June 2019

As a cause of accidents, drowsiness can cause economical and physical damage. A range of drowsiness estimation methods have been proposed in previous studies to aid accident prevention and address this problem. However, none of these methods are able to improve their estimation ability as the length of time or number of trials increases. Thus, in this study, we aim to find an effective drowsiness estimation method that is also able to improve its prediction ability as the subject’s activity increases. We used electroencephalogram (EEG) data to estimate drowsiness, and the Karolinska sleepiness scale (KSS) for drowsiness evaluation. Five parameters (α, β/α, (θ+α)/β, activity, and mobility) from the O1 electrode site were selected. By combining these parameters and KSS, we demonstrate that a typical support vector regression (SVR) algorithm can estimate drowsiness with a correlation coefficient (R2) of up to 0.64 and a root mean square error (RMSE) of up to 0.56. We propose a “recurrent SVR” (RSVR) method with improved estimation performance, as highlighted by an R2 value of up to 0.83, and an RMSE of up to 0.15. These results suggest that in addition to being able to estimate drowsiness based on EEG data, RSVR is able to improve its drowsiness estimation performance.
Alteration of Epigenetic Modifiers in Pancreatic Cancer and Its Clinical Implication
Authors: Hung Yu-Hsuan , Hsu Ming-Chuan , Chen Li-Tzong , Hung Wen-Chun , Pan Mei-Ren
Published: June 2019

The incidence of pancreatic cancer has considerably increased in the past decade. Pancreatic cancer has the worst prognosis among the cancers of the digestive tract because the pancreas is located in the posterior abdominal cavity, and most patients do not show clinical symptoms for early detection. Approximately 55% of all patients are diagnosed with pancreatic cancer only after the tumors metastasize. Therefore, identifying useful biomarkers for early diagnosis and screening high-risk groups are important to improve pancreatic cancer therapy. Recent emerging evidence has suggested that genetic and epigenetic alterations play a crucial role in the molecular aspects of pancreatic tumorigenesis. Here, we summarize recent progress in our understanding of the epigenetic alterations in pancreatic cancer and propose potential synthetic lethal strategies to target these genetic defects to treat this deadly disease.
Moderate and Stable Pain Reductions as a Result of Interdisciplinary Pain Rehabilitation—A Cohort Study from the Swedish Quality Registry for Pain Rehabilitation (SQRP)
Authors: Ringqvist Åsa , Dragioti Elena , Björk Mathilda , Larsson Britt , Gerdle Björn
Published: June 2019

Few studies have investigated the real-life outcomes of interdisciplinary multimodal pain rehabilitation programs (IMMRP) for chronic pain. This study has four aims: investigate effect sizes (ES); analyse correlation patterns of outcome changes; define a multivariate outcome measure; and investigate whether the clinical self-reported presentation pre-IMMRP predicts the multivariate outcome. To this end, this study analysed chronic pain patients in specialist care included in the Swedish Quality Registry for Pain Rehabilitation for 22 outcomes (pain, psychological distress, participation, and health) on three occasions: pre-IMMRP, post-IMMRP, and 12-month follow-up. Moderate stable ES were demonstrated for pain intensity, interference in daily life, vitality, and health; most other outcomes showed small ES. Using a Multivariate Improvement Score (MIS), we identified three clusters. Cluster 1 had marked positive MIS and was associated with the overall worst situation pre-IMMRP. However, the pre-IMMRP situation could only predict 8% of the variation in MIS. Specialist care IMPRPs showed moderate ES for pain, interference, vitality, and health. Outcomes were best for patients with the worst clinical presentation pre-IMMRP. It was not possible to predict who would clinically benefit most from IMMRP.
Comparison of Radio Frequency Path Loss Models in Soil for Wireless Underground Sensor Networks
Authors: Abdorahimi Danial , Sadeghioon Ali M.
Published: June 2019

A wireless Underground Sensor Network (WUSN) is a group of sensors that collectively communicate through the underground medium. Radio Frequency (RF) signal transmission of the sensors through the ground is the most challenging aspects of a WUSN due to the high attenuation of the electromagnetic (EM) signal in the soil. Signals are often required to travel through soils with a high density or water content and generally through a non-isotropic and non-homogenous soil mixture with different boundaries, both of which can attenuate the signal sharply. The variability of the these conditions and complexity of the behaviour of signal attenuation with respect to these parameters makes accurate estimation of EM signal attenuation in soil challenging. Two main EM signal attenuation models exist to estimate attenuation (modified-Friis and Complex Refractive Index Model-Fresnel (CRIM-Fresnel). These were reviewed and a methodology was developed in order to measure the attenuation of the EM signals in the laboratory. Results from the laboratory measurements were compared with the estimation values calculated from the attenuation models. These comparisons showed a large difference between the estimated values by the models. In addition, analysis of the comparison tests showed that the CRIM-Fresnel model provides a better estimation of attenuation in samples with lower permittivity values while the modified-Friis model had a higher accuracy in samples with higher clay/water content which have higher permittivity values.
Influence of the Nonequilibrium Material State on Wear Resistance
Authors: Gershman Iosif , Mironov Alexander , Veldhuis Stephen , Gershman Eugeniy I. , Podrabinnik Pavel , Kuznetsova Ekaterina
Published: June 2019

The influence of the nonequilibrium state of a material on its wear resistance is investigated in this study. Using methods of non-equilibrium thermodynamics and the theory of self-organization, a non-equilibrium material is shown to possess an overall lower wear rate than a material in an equilibrium state. This was experimentally demonstrated to be the case in different materials and tribosystems, such as Babbit operating in complete lubrication conditions, copper in the current collector and a cutting tool with a coating applied on it.
Textile Display with AMOLED Using a Stacked-Pixel Structure on a Polyethylene Terephthalate Fabric Substrate
Authors: Kim Jae Seon , Song Chung Kun
Published: June 2019

An active-mode organic light-emitting diode (AMOLED) display on a fabric substrate is expected to be a prominent textile display for e-textile applications. However, the large surface roughness of the fabric substrate limits the aperture ratio—the area ratio of the organic light-emitting diode (OLED) to the total pixel area. In this study, the aperture ratio of the AMOLED panel fabricated on the polyethylene terephthalate fabric substrate was enhanced by applying a stacked-pixel structure, in which the OLED was deposited above the organic thin-film transistor (OTFT) pixel circuit layer. The stacked pixels were achieved using the following three key technologies. First, the planarization process of the fabric substrate was performed by sequentially depositing a polyurethane and photo-acryl layer, improving the surface roughness from 10 μm to 0.3 μm. Second, a protection layer consisting of three polymer layers, a water-soluble poly-vinyl alcohol, dichromated-polyvinylalcohol (PVA), and photo acryl, formed by a spin-coating processes was inserted between the OTFT circuit and the OLED layer. Third, a high mobility of 0.98 cm2/V∙s was achieved at the panel scale by using hybrid carbon nano-tube (CNT)/Au (5 nm) electrodes for the S/D contacts and the photo-acryl (PA) for the gate dielectric, enabling the supply of a sufficiently large current (40 μA @ VGS = −10 V) to the OLED. The aperture ratio of the AMOLED panel using the stacked-pixel structure was improved to 48%, which was about two times larger than the 19% of the side-by-side pixel, placing the OLED just beside the OTFTs on the same plane.
Deformation of Single Crystals, Polycrystalline Materials, and Thin Films: A Review
Authors: Yang Guijun , Park Soo-Jin
Published: June 2019

With the rapid development of nano-preparation processes, nanocrystalline materials have been widely developed in the fields of mechanics, electricity, optics, and thermal physics. Compared to the case of coarse-grained or amorphous materials, plastic deformation in nanomaterials is limited by the reduction in feature size, so that they generally have high strength, but the toughness is relatively high. The “reciprocal relationship” between the strength and toughness of nanomaterials limits the large-scale application and development of nanomaterials. Therefore, the maintenance of high toughness while improving the strength of nanomaterials is an urgent problem to be solved. So far, although the relevant mechanism affecting the deformation of nanocrystalline materials has made a big breakthrough, it is still not very clear. Therefore, this paper introduces the basic deformation type, mechanism, and model of single crystals, polycrystalline materials, and thin films, and aims to provide literature support for future research.
Efficient 1 µm Laser Emission of Czochralski-Grown Nd:LGSB Single Crystal
Authors: Brandus Catalina-Alice , Hau Stefania , Broasca Alin , Greculeasa Madalin , Voicu Flavius-Marian , Gheorghe Cristina , Gheorghe Lucian , Dascalu Traian
Published: June 2019

A 5.0-at.% Nd-doped La0.64Gd0.41Sc2.95(BO3)4 (Nd:LGSB) borate laser crystal was successfully grown by the Czochralski method, for the first time to our knowledge. The spectroscopic properties of the grown crystal are discussed and 1 µm laser emission, under end-pumping with a fiber-coupled diode laser at 807 nm, is reported. A c-cut Nd:LGSB medium yielded 1.35 W continuous-wave output power at 0.63 overall optical-to-optical efficiency, with respect to the absorbed pump power, together with the high 0.68 slope efficiency. With an a-cut Nd:LGSB sample, 0.81 W output power at 0.52 optical-to-optical efficiency was obtained. The laser emission performances under quasi-continuous wave pumping are presented as well, for both c-cut and a-cut crystals. Passive Q-switching was investigated with a semiconductor saturable absorber mirror (SESAM). Laser pulses with 2.2 µJ energy and 32.8 ns durations were recorded from a-cut Nd:LGSB. The average output power reached 0.36 W at 1.55 W absorbed pump power. Passive mode-locking with SESAM was achieved in a long Z-type resonator. Ultrashort pulses with 0.19 W average power, 1.63 nJ energy, and 1.43 ps pulse duration, at 118 MHz repetition rate, are demonstrated for the a-cut Nd:LGSB medium.
Bending Flexibility of Moso Bamboo (Phyllostachys Edulis) with Functionally Graded Structure
Authors: Wei Xin , Zhou Haiying , Chen Fuming , Wang Ge
Published: June 2019

As one of the most renewable and sustainable resources on Earth, bamboo with its high flexibility has been used in the fabrication of a wide variety of composite structures due to its properties. A bamboo-based winding composite (BWC) is an innovative bamboo product which has revolutionized pipe structures and their applications throughout China as well as improving their impact on the environment. However, as a natural functionally graded composite, the flexibility mechanism of bamboo has not yet been fully understood. Here, the bending stiffness method based on the cantilever beam principle was used to investigate the gradient and directional bending flexibility of bamboo (Phyllostachys edulis) slivers under different loading Types during elastic stages. Results showed that the graded distribution and gradient variation of cell size of the fibers embedded in the parenchyma cells along the thickness of the bamboo culm was mainly responsible for the exhibited gradient bending flexibility of bamboo slivers, whereas the shape and size difference of the vascular bundles from inner to outer layers played a critical role in directional bending flexibility. A validated rule of mixture was used to fit the bending stiffness under different loading Types as a function of fiber volume fraction. This work provides insights to the bionic preparation and optimization of high-performance BWC pipes.
A Comparative Study between Knocked-Down Aligned Carbon Nanotubes and Buckypaper-Based Strain Sensors
Authors: Santos Ana , Amorim Luís , Nunes João Pedro , Rocha Luís Alexandre , Silva Alexandre Ferreira , Viana Júlio César
Published: June 2019

Carbon nanotubes (CNTs) are one of the most promising materials in sensing applications due to their electrical and mechanical properties. This paper presents a comparative study between CNT Buckypaper (BP) and aligned CNT-based strain sensors. The Buckypapers were produced by vacuum filtration of commercial CNTs dispersed in two different solvents, N,N-Dimethylformamide (DMF) and ethanol, forming freestanding sheets, which were cut in 10 × 10 mm squares and transferred to polyimide (PI) films. The morphology of the BP was characterized by scanning electron microscopy (SEM). The initial electrical resistivity of the samples was measured, and then relative electrical resistance versus strain measurements were obtained. The results were compared with the knocked-down vertically aligned CNT/PI based sensors previously reported. Although both types of sensors were sensitive to strain, the aligned CNT/PI samples had better mechanical performance and the advantage of inferring strain direction due to their electrical resistivity anisotropic behavior.
Electrospun Bilayer Chitosan/Hyaluronan Material and Its Compatibility with Mesenchymal Stem Cells
Authors: Petrova Valentina A. , Chernyakov Daniil D. , Poshina Daria N. , Gofman Iosif V. , Romanov Dmitry P. , Mishanin Alexander I. , Golovkin Alexey S. , Skorik Yury A.
Published: June 2019

A bilayer nonwoven material for tissue regeneration was prepared from chitosan (CS) and hyaluronic acid (HA) by needleless electrospinning wherein 10–15 wt% (with respect to polysaccharide) polyethylene oxide was added as spinning starter. A fiber morphology study confirmed the material’s uniform defect-free structure. The roughness of the bilayer material was in the range of 1.5–3 μm, which is favorable for cell growth. Electrospinning resulted in the higher orientation of the polymer structure compared with that of corresponding films, and this finding may be related to the orientation of the polymer chains during the spinning process. These structural changes increased the intermolecular interactions. Thus, despite a high swelling degree of 1.4–2.8 g/g, the bilayer matrix maintained its shape due to the large quantity of polyelectrolyte contacts between the chains of oppositely charged polymers. The porosity of the bilayer CS–HA nonwoven material was twice lower, while the Young’s modulus and break stress were twice higher than that of a CS monolayer scaffold. Therefore, during the electrospinning of the second layer, HA may have penetrated into the pores of the CS layer, thereby increasing the polyelectrolyte contacts between the two polymers. The bilayer CS–HA scaffold exhibited good compatibility with mesenchymal stem cells. This characteristic makes the developed material promising for tissue engineering applications.
Influence of Pore Size Variation on Thermal Conductivity of Open-Porous Foams
Authors: Skibinski Jakub , Cwieka Karol , Haj Ibrahim Samih , Wejrzanowski Tomasz
Published: June 2019

This study addresses the influence of pore size variation on the effective thermal conductivity of open-cell foam structures. Numerical design procedure which renders it possible to control chosen structural parameters has been developed based on characterization of commercially available open-cell copper foams. Open-porous materials with various pore size distribution were numerically designed using the Laguerre–Voronoi Tessellations procedure. Heat transfer through an isolated structure was simulated with the finite element method. The results reveal that thermal conductivity is strongly related to porosity, which is in agreement with the literature. The influence of pore size distribution has also been observed and compared with analytical formulas proposed in the literature.
Zeolite Adsorption of Chloride from a Synthetic Alkali-Activated Cement Pore Solution
Authors: Osio-Norgaard Jorge , Srubar Wil V.
Published: June 2019

This work presents experimental evidence that confirms the potential for two specific zeolites, namely chabazite and faujasite (with a cage size ~2–13 Å), to adsorb small amounts of chloride from a synthetic alkali-activated cement (AAC) pore solution. Four synthetic zeolites were first exposed to a chlorinated AAC pore solution, two faujasite zeolites (i.e., FAU, X-13), chabazite (i.e., SSZ-13), and sodium-stabilized mordenite (i.e., Na-Mordenite). The mineralogy and chemical composition were subsequently investigated via X-ray diffraction (XRD) and both energy- and wavelength-dispersive X-ray spectroscopy (WDS), respectively. Upon exposure to a chlorinated AAC pore solution, FAU and SSZ-13 displayed changes to their diffraction patterns (i.e., peak shifting and broadening), characteristic of ion entrapment within zeolitic aluminosilicate frameworks. Elemental mapping with WDS confirmed the presence of small amounts of elemental chlorine. Results indicate that the chloride-bearing capacity of zeolites is likely dependent on both microstructural features (e.g., cage sizes) and chemical composition.
Research of Method for Solving Relaxation Modulus Based on Three-Point Bending Creep Test
Authors: Sun Yazhen , Gu Zhangyi , Wang Jinchang , Yuan Xuezhong
Published: June 2019

A method was developed for solving the relaxation modulus of high viscosity asphalt sand (HVAS) based on the three-point bending creep test, and was verified by comparison with experimental results. In this method, firstly, a transcendental equation was obtained by the convolution, and then equations were obtained by Taylor’s formula, which were solved by Mathmatica to obtain the relaxation modulus by Newton’s method. Subsequently, the laboratory investigations of the viscoelastic parameters of the Burgers model for the HVAS by three-point bending creep tests were carried out. In addition, the method was verified by comparing the relaxation moduli with the indoor relaxation experiments. Results showed that the numerical calculation and the test data were in good agreement, and the relaxation characteristics of the HVAS were reflected more accurately. The method can be used to study the relaxation characteristics of the asphalt mixtures effectively. In addition, this study provides a research basis for road crack prevention.
Non-Destructive Testing of Technical Conditions of RC Industrial Tall Chimneys Subjected to High Temperature
Authors: Maj Marek , Ubysz Andrzej , Hammadeh Hala , Askifi Farzat
Published: June 2019

Non-destructive tests of reinforced concrete chimneys, especially high ones, are an important element in assessing their condition, making it possible to forecast their safe life. Industrial chimneys are often exposed to the strong action of acidic substances, They are negatively exposed to the condensation of the flue gases. Condensate affects the inside of the thermal insulation and penetrates the chimney wall from the outside. This is one reason for the corrosion of concrete and reinforcing steel. Wet thermal insulation settles, and drastically reduces its insulating properties. This leads to an increase in temperature in the reinforced concrete chimney wall and creates additional large variations in temperature fields. This consequently causes a large increase in internal forces, which mainly increase tensile and shear stresses. This results in the appearance of additional cracks in the wall. The acid condensate penetrates these cracks, destroying the concrete cover and reinforcement. Thermographic studies are very helpful in monitoring the changes in temperature and consequently, the risk of concrete and reinforcement corrosion. This simple implication between changes in temperature of the chimney wall and increasing inner forces as shown in this article is particularly important when the chimney cannot be switched off due to the nature of the production process. Methods for interpreting the results of thermovision tests are presented to determine the safety and durability of industrial chimneys.