bluuebunny/crossref_metadata_2025 huggingface dataset:
A Streamlined Approach to Pathway Analysis from RNA-Sequencing Data
Authors: Bow Austin
Published: March 2021

The reduction in costs associated with performing RNA-sequencing has driven an increase in the application of this analytical technique; however, restrictive factors associated with this tool have now shifted from budgetary constraints to time required for data processing. The sheer scale of the raw data produced can present a formidable challenge for researchers aiming to glean vital information about samples. Though many of the companies that perform RNA-sequencing provide a basic report for the submitted samples, this may not adequately capture particular pathways of interest for sample comparisons. To further assess these data, it can therefore be necessary to utilize various enrichment and mapping software platforms to highlight specific relations. With the wide array of these software platforms available, this can also present a daunting task. The methodology described herein aims to enable researchers new to handling RNA-sequencing data with a streamlined approach to pathway analysis. Additionally, the implemented software platforms are readily available and free to utilize, making this approach viable, even for restrictive budgets. The resulting tables and nodal networks will provide valuable insight into samples and can be used to generate high-quality graphics for publications and presentations.
GABr Post-Treatment for High-Performance MAPbI3 Solar Cells on Rigid Glass and Flexible Substrate
Authors: Chen Tingting , He Rui , Zhang Fan , Hao Xia , Xuan Zhipeng , Wang Yunfan , Wang Wenwu , Zhao Dewei , Zhang Jingquan , Wu Lili
Published: March 2021

Perovskite solar cells have exhibited astonishing photoelectric conversion efficiency and have shown a promising future owing to the tunable content and outstanding optoelectrical property of hybrid perovskite. However, the devices with planar architecture still suffer from huge Voc loss and severe hysteresis effect. In this research, Guanidine hydrobromide (GABr) post-treatment is carried out to enhance the performance of MAPbI3 n-i-p planar perovskite solar cells. The detailed characterization of perovskite suggests that GABr post-treatment results in a smoother absorber layer, an obvious reduction of trap states and optimized energy level alignment. By utilizing GABr post-treatment, the Voc loss is reduced, and the hysteresis effect is alleviated effectively in MAPbI3 solar cells. As a result, solar cells based on glass substrate with efficiency exceeding 20%, Voc of 1.13 V and significantly mitigated hysteresis are fabricated successfully. Significantly, we also demonstrate the effectiveness of GABr post-treatment in flexible device, whose efficiency is enhanced from 15.77% to 17.57% mainly due to the elimination of Voc loss.
Comparison of Ketogenic Diets with and without Ketone Salts versus a Low-Fat Diet: Liver Fat Responses in Overweight Adults
Authors: Crabtree Christopher , Kackley Madison , Buga Alexandru , Fell Brandon , LaFountain Richard , Hyde Parker , Sapper Teryn , Kraemer William , Scandling Debbie , Simonetti Orlando , Volek Jeff
Published: March 2021

Ketogenic diets (KDs) often contain high levels of saturated fat, which may increase liver fat, but the lower carbohydrate intake may have the opposite effect. Using a controlled feeding design, we compared liver fat responses to a hypocaloric KD with a placebo (PL) versus an energy-matched low-fat diet (LFD) in overweight adults. We also examined the added effect of a ketone supplement (KS). Overweight adults were randomized to a 6-week KD (KD + PL) or a KD with KS (KD + KS); an LFD group was recruited separately. All diets were estimated to provide 75% of energy expenditure. Weight loss was similar between groups (p > 0.05). Liver fat assessed by magnetic resonance imaging decreased after 6 week (p = 0.004) with no group differences (p > 0.05). A subset with nonalcoholic fatty liver disease (NAFLD) (liver fat > 5%, n = 12) showed a greater reduction in liver fat, but no group differences. In KD participants with NAFLD, 92% of the variability in change in liver fat was explained by baseline liver fat (p < 0.001). A short-term hypocaloric KD high in saturated fat does not adversely impact liver health and is not impacted by exogenous ketones. Hypocaloric low-fat and KDs can both be used in the short-term to significantly reduce liver fat in individuals with NAFLD.
Iron Protein Succinylate in the Management of Iron Deficiency Anemia: A Comparative Study with Ferrous Sulphate at Low and High Therapeutic Doses
Authors: Urso Katia , Leal Martínez-Bujanda Javier , del Prado Jaime Moscoso
Published: March 2021

Oral iron supplementation constitutes the first line treatment for iron deficiency anemia (IDA), with daily doses between 80 mg and 200 mg of elemental iron. Ferrous salts, such as ferrous sulphate (FeSO4), while efficacious, frequently give rise to gastrointestinal side effects. In the present paper we attempted to directly compare the efficacy of an alternative to the FeSO4 formulation, which presents a better tolerability profile, iron protein succinylate (Ferplex®). In a diet-induced anemia model, rats were treated by oral gavage with vehicle, FeSO4, or Ferplex® at a human-dose equivalent of 80 mg and 200 mg of elemental iron. We evaluated the change in anemia-related hematological and biochemical parameters, conducting a histological examination of the intestine at sacrifice. Results indicate that both types of iron supplementation are equally effective in the treatment of IDA, restoring hemoglobin, hematocrit, erythrocytes, free iron and transferrin levels in 15 days, with no statistical differences between treated groups and control. The impact of anemia on body weight was also attenuated following treatment with both iron supplements. Thrombocyte and reticulocyte levels, altered by the anemic condition, returned to homeostasis after 15 days of either FeSO4 or Ferplex® treatment. Importantly, the lower and higher doses of iron were equally effective, thus supporting the current school of thought which states that lower therapeutic doses are sufficient for management of IDA. In addition, the study shows for the first time that oral treatment with Ferplex® does not increase serum hepcidin. Finally, Ferplex® induced minimal iron depositions in the intestinal tissue compared to FeSO4.
Cloudy Apple Juice Fermented by Lactobacillus Prevents Obesity via Modulating Gut Microbiota and Protecting Intestinal Tract Health
Authors: Han Mengzhen , Zhang Meina , Wang Xiaowei , Bai Xue , Yue Tianli , Gao Zhenpeng
Published: March 2021

Obesity and hyperglycemia are two serious chronic diseases that are increasing in incidence worldwide. This research aimed to develop a fermented cloudy apple juice with good hyperglycemia intervention activities. Here, cloudy apple juice (CAJ), cloudy apple juice rich in polyphenols (CAJP) and fermented cloudy apple juice rich in polyphenols (FCAJP) were prepared sequentially, and then the effects of the three apple juices on weight, lipid level, gut microbiota composition and intestinal tract health were evaluated for obese mice induced by a high-fat diet. The research findings revealed that the FCAJP showed potential to inhibit the weight gain of mice, reduce fat accumulation, and regulate the blood lipid levels of obese mice by decreasing the ratio of the Firmicutes/Bacteroidotas, improving the Sobs, Ace, and Chao indexes of the gut microbiota and protecting intestinal tract health. In addition, the FCAJP augmented the abundance of Akkermansia and Bacteroides, which were positively related to SCFAs in cecal contents. This study inferred that FCAJP could be developed as a healthy food for preventing obesity and hyperglycemia.
Effect of a Food for Special Medical Purposes for Muscle Recovery, Consisting of Arginine, Glutamine and Beta-Hydroxy-Beta-Methylbutyrate on Body Composition and Skin Health in Overweight and Obese Class I Sedentary Postmenopausal Women
Authors: Rondanelli Mariangela , Nichetti Mara , Peroni Gabriella , Naso Maurizio , Faliva Milena Anna , Iannello Giancarlo , Di Paolo Enrica , Perna Simone
Published: March 2021

The consumption of dietary amino acids has been evaluated for therapeutic and safety intervention in obesity. In particular, three molecules have been shown to be effective: arginine, glutamine and leucine (and its metabolite beta-hydroxy-beta-methylbutyrate, HMB). This randomized, double-blinded pilot study in obese postmenopausal patients aimed to evaluate the efficacy of the administration of a specific food for special medical purposes (FSMP) consisting of arginine, glutamine and HMB on body composition, in particular, visceral adipose tissue (VAT), assessed by dual-energy X-ray absorptiometry (DXA), as the primary endpoint. The secondary endpoint was to evaluate the effects on skin health through a validated self-reported questionnaire. A significant improvement on VAT of Δ = −153.600, p = 0.01 was recorded in the intervention group. Skin health showed a significant improvement in the treatment group for the following: bright Δ = 1.400 (0.758; 2.042), elasticity Δ = 0.900 (0.239; 1.561), wrinkles Δ = 0.800 (0.276; 1.324), and on total score, Δ = 3.000 (1.871; 4.129). In the intervention group, the improvement in VAT was associated with an improvement in the bright score (r = −0.58; p = 0.01). In conclusion, this study demonstrated that the intake for 4-weeks of arginine, glutamine and HMB effects a significant reduction in VAT and improves skin condition, while fat free mass (FFM) is maintained, thus achieving “high-quality” weight loss.
Publication Trends in Neglected Tropical Diseases of Latin America and the Caribbean: A Bibliometric Analysis
Authors: Fontecha Gustavo , Sánchez Ana , Ortiz Bryan
Published: March 2021

(1) Background: Neglected tropical diseases (NTDs) have been overlooked on the global health agenda and in the priorities of national systems in low- and middle-income countries (LMICs). In 2012, the Sustainable Development Goals (SDGs) were created to ensure healthy lives and promoting well-being for all. This roadmap set out to accelerate work to overcome the global impact of NTDs. Almost a decade has passed since NTDs were re-launched as a global priority. Investment in research and development, as well as the production of scientific literature on NTDs, is expected to have increased significantly. (2) Methods: A bibliometric analysis of the scientific production of Latin America and the Caribbean (LAC) was carried out in relation to 19 endemic NTDs. These data were compared with the scientific production in malaria, tuberculosis, and HIV/AIDS. The database available from Thomson Reuters Web of Science (WoS) was used. In addition, the average annual growth percentage was calculated for each disease. (3) Results: In the last decade, the NTDs with the highest number of publications in the world were dengue and leishmaniasis. The United States was the most prolific country in the world in 15 out of 19 NTDs analyzed. In the LAC region, Brazil was the largest contributor for 16 of the 19 NTDs analyzed. Arboviral diseases showed the highest average annual growth. The number of publications for malaria, tuberculosis and HIV/AIDS was considerably higher than for NTDs. The contribution of most LAC countries, especially those considered to be LMICs, is inadequate and does not reflect the relevance of NTDs for the public health of the population. (4) Conclusions: This is the first bibliometric analysis to assess the trend of scientific documents on endemic NTDs in LAC. Our results could be used by decision makers both to strengthen investment policies in research and development in NTDs.
Effects of GPR18 Ligands on Body Weight and Metabolic Parameters in a Female Rat Model of Excessive Eating
Authors: Kotańska Magdalena , Mika Kamil , Szafarz Małgorzata , Kubacka Monika , Müller Christa E. , Sapa Jacek , Kieć-Kononowicz Katarzyna
Published: March 2021

GPR18 has been proposed to play a role in the progression of metabolic disease and obesity. Therefore, the aim of this study was to determine the effects of selective GRP18 ligands (the antagonists PSB-CB5 and PSB-CB27 and the agonist PSB-KK1415) on body mass and the development of metabolic disorders commonly accompanying obesity. Experiments were carried out on female Wistar rats. In order to determine the anorectic activity of the investigated ligands, their effect on food and water intake in a model of excessive eating was assessed. Lipid profile, glucose and insulin levels as well as alanine aminotransferase, aspartate aminotransferase, and γ-glutamyl transpeptidase activity in plasma were also evaluated. Potential side effects were examined in rat models of pica behavior and conditioned taste aversion. Animals treated with different ligands gained significantly less weight than rats from the obese control group. Effects of GPR18 antagonists on food intake and body weight were specific and unrelated to visceral illness, stress or changes in spontaneous activity. However, the GPR18 agonist is likely to affect body weight by inducing gastrointestinal disorders such as nausea. The presented preliminary data support the idea that the search for selective GPR18 antagonists for the treatment of obesity might be promising.
Acute MDPV Binge Paradigm on Mice Emotional Behavior and Glial Signature
Authors: Campeão Mafalda , Fernandes Luciana , Pita Inês R. , Lemos Cristina , Ali Syed F. , Carvalho Félix , Rodrigues-Santos Paulo , Fontes-Ribeiro Carlos A. , Soares Edna , Viana Sofia D. , Pereira Frederico C.
Published: March 2021

3,4-Methylenedioxypyrovalerone (MDPV), a widely available synthetic cathinone, is a popular substitute for classical controlled drugs of abuse, such as methamphetamine (METH). Although MDPV poses public health risks, its neuropharmacological profile remains poorly explored. This study aimed to provide evidence on that direction. Accordingly, C57BL/6J mice were exposed to a binge MDPV or METH regimen (four intraperitoneal injections every 2 h, 10 mg/kg). Locomotor, exploratory, and emotional behavior, in addition to striatal neurotoxicity and glial signature, were assessed within 18–24 h, a known time-window encompassing classical amphetamine dopaminergic neurotoxicity. MDPV resulted in unchanged locomotor activity (open field test) and emotional behavior (elevated plus maze, splash test, tail suspension test). Additionally, striatal TH (METH neurotoxicity hallmark), Iba-1 (microglia), GFAP (astrocyte), RAGE, and TLR2/4/7 (immune modulators) protein densities remained unchanged after MDPV-exposure. Expectedly, and in sheer contrast with MDPV, METH resulted in decrease general locomotor activity paralleled by a significant striatal TH depletion, astrogliosis, and microglia arborization alterations (Sholl analysis). This comparative study newly highlights that binge MDPV-exposure comes without evident behavioral, neurochemical, and glial changes at a time-point where METH-induced striatal neurotoxicity is clearly evident. Nevertheless, neuropharmacological MDPV signature needs further profiling at different time-points, regimens, and brain regions.
Evaluation of Hydroxychloroquine Blood Concentrations and Effects in Childhood-Onset Systemic Lupus Erythematosus
Authors: Zahr Noël , Urien Saik , Funck-Brentano Christian , Vantomme Hélène , Garcelon Nicolas , Melki Isabelle , Boistault Margaux , Boyer Olivia , Bader-Meunier Brigitte
Published: March 2021

Background: Hydroxychloroquine (HCQ) is an antimalarial agent given to patients with systemic lupus erythematosus (SLE) as first-line therapy. It alleviates childhood-onset systemic lupus erythematosus cSLE skin and musculoskeletal disease, decreasing disease activity and flares. HCQ concentration–effect relationships in children remains unknown. This study aimed to investigate the pharmacokinetics of HCQ and possible concentration–effect relationships. Methods: HCQ blood concentrations and effects were obtained during clinical follow-up on different occasions. cSLE flares were defined using the SLE Disease Activity Index (SLEDAI); flare was denoted by a SLEDAI score > 6. Blood concentration was measured using high-performance liquid chromatography with fluorometric detection. Statistical analysis was performed using a nonlinear mixed-effect approach with the Monolix software. Results: A total of 168 blood samples were obtained from 55 pediatric patients. HCQ apparent blood clearance (CL/F) was dependent on patients’ bodyweight and platelet count. Patients with active cSLE had a lower mean blood HCQ concentration compared with inactive cSLE patients (536 ± 294 vs. 758 ± 490 ng/mL, p = 5 × 10−6). Among patients with HCQ blood concentration ≥750 ng/mL, 87.6% had inactive cSLE. Moreover, HCQ blood concentration was a significant predictor of disease status. Conclusion: We developed the first HCQ blood concentration–effect relationship for cSLE associated with active or non-active disease status. A prospective randomized study is necessary to confirm these results.
The Ins and Outs of Messenger RNA Electroporation for Physical Gene Delivery in Immune Cell-Based Therapy
Authors: Campillo-Davo Diana , De Laere Maxime , Roex Gils , Versteven Maarten , Flumens Donovan , Berneman Zwi N. , Van Tendeloo Viggo F. I. , Anguille Sébastien , Lion Eva
Published: March 2021

Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each cell type as well as introduction of multiple protein-coding mRNAs at once. As a pioneering group in mRNA electroporation, in this review, we provide an expert overview of the ins and outs of mRNA electroporation, discussing the different parameters involved in mRNA electroporation as well as the production of research-grade and production and application of clinical-grade mRNA for gene transfer in the context of cell-based immunotherapies.
Umbilical Cord, Maternal Milk, and Breastfed Infant Levetiracetam Concentrations Monitoring at Delivery and during Early Postpartum Period
Authors: Kacirova Ivana , Grundmann Milan , Brozmanova Hana
Published: March 2021

(1) To obtain objective information about levetiracetam transplacental passage and its transport into colostrum, mature milk, and breastfed infants, we analyzed data from women treated for epilepsy between October 2006 and January 2021; (2) in this cohort study, maternal, umbilical cord, milk, and infant serum concentrations were measured at delivery, 2–4 days postpartum (colostrum) and 7–31 days postpartum (mature milk). Paired umbilical cord serum, maternal serum, breastfed infant serum, and milk concentrations were used to assess the ratios of umbilical cord/maternal serum, milk/maternal serum, and infant/maternal serum concentrations. The influence of combined treatment with enzyme-inducing antiseizure medication carbamazepine was assessed; (3) the umbilical cord/maternal serum concentration ratio ranged between 0.75 and 1.78 (mean 1.10 ± 0.33), paired maternal and umbilical cord serum concentrations were not significantly different, and a highly significant correlation was found between both concentrations. The mean milk/maternal serum concentration ratio was 1.14 ± 0.27 (2–4 days postpartum) and 1.04 ± 0.24 (7–31 days postpartum) while the mean infant/maternal serum concentration ratio was markedly lower (0.19 ± 0.13 and 0.14 ± 0.05, respectively); (4) levetiracetam was found in the umbilical cord at a concentration similar to those in maternal serum. All of the breastfed infant serum concentrations were below the reference range used for the general epileptic population.
Proceedings of the 7th Series of Seminars on Advances in Apomixis Research
Authors: Echenique Viviana , Autran Daphné , Leblanc Olivier
Published: March 2021

These proceedings contain the abstracts for the presentations given at the 7th biennial Seminars on Advances in Apomixis Research, held virtually on 2–3 and 9 December 2020. The first day hosted the kick-off meeting of the EU-funded Mechanisms of Apomictic Development (MAD) project, while the remaining days were dedicated to oral presentations and in-depth exchanges on the latest progress in the field of apomixis and plant reproductive biology research.
The Effects of Crosslinking on the Rheology and Cellular Behavior of Polymer-Based 3D-Multilayered Scaffolds for Restoring Articular Cartilage
Authors: Campos Yaima , Sola Francisco J. , Fuentes Gastón , Quintanilla Luis , Almirall Amisel , Cruz Luis J. , Rodríguez-Cabello José C. , Tabata Yasuhiko
Published: March 2021

Polymer-based tri-layered (bone, intermediate and top layers) scaffolds used for the restoration of articular cartilage were prepared and characterized in this study to emulate the concentration gradient of cartilage. The scaffolds were physically or chemically crosslinked. In order to obtain adequate scaffolds for the intended application, the impact of the type of calcium phosphate used in the bone layer, the polymer used in the intermediate layer and the interlayer crosslinking process were analyzed. The correlation among SEM micrographs, physical-chemical characterization, swelling behavior, rheological measurements and cell studies were examined. Storage moduli at 1 Hz were 0.3–1.7 kPa for physically crosslinked scaffolds, and 4–5 kPa (EDC/NHS system) and 15–20 kPa (glutaraldehyde) for chemically crosslinked scaffolds. Intrinsic viscoelasticity and poroelasticity were considered in discussing the physical mechanism dominating in different time/frequency scales. Cell evaluation showed that all samples are available as alternatives to repair and/or substitute cartilage in articular osteoarthritis.
Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
Authors: Ghorbanzadeh Sheish Shahnaz , Emadi Rahmatollah , Ahmadian Mehdi , Sadeghzade Sorour , Tavangarian Fariborz
Published: March 2021

One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber–polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize the polyvinylpyrrolidone-acrylic acid hydrogel (PVPA)–eggshell membrane (ESM)–reduced graphene oxide (rGO) nanosheets nanocomposite dressings with different reduced graphene oxide contents (0, 0.5, 1, and 2 wt.%). Thus, smooth nanofibers were fabricated, including a high amount of rGO, which reduced the fiber diameter. Based on the results, rGO played an important role in water impermeability. The results showed that by increasing the rGO concentration from 0.5 to 2 wt%, the contact angle value increased persistently. Results showed that compared to PVPA–ESM, the mechanical strength and strain of PVPA–ESM/1 wt% rGO significantly enhanced 28% and 23%, respectively. Incorporation of 1 wt% rGO enhanced swelling ratio from 875% for PVPA-ESM to 1235% after 420 min, while increasing the rGO to 2 wt% increased the degradation rate of the composites. According to the in vitro cell culture studies, PVPA-ESM wound dressings with 0.5–1 wt% rGO content enhanced PC12 cell viability compared to the wound dressings without rGO nanosheets. Generally, rGO–loaded PVPA-ESM nanofiber wound dressing can be considered as a potential candidate to be used in skin regeneration applications.
Plumbagin-Loaded Glycerosome Gel as Topical Delivery System for Skin Cancer Therapy
Authors: Md Shadab , Alhakamy Nabil A. , Aldawsari Hibah M. , Husain Mohammad , Khan Nazia , Alfaleh Mohamed A. , Asfour Hani Z. , Riadi Yassine , Bilgrami Anwar L. , Akhter Md Habban
Published: March 2021

Plumbagin (PLM) is a phytochemical which has shown cytotoxicity against of cancer cells both in vitro and in vivo. However, the clinical application of PLM has been hindered due to poor aqueous solubility and low bioavailability. The aim of the present study was to develop, optimize and evaluate PLM-loaded glycerosome (GM) gel and compare with conventional liposome (CL) for therapeutic efficacy against skin cancer. The GM formulations were optimized by employing design expert software by 3-level 3-factor design. The prepared GMs were characterized in vitro for vesicle size, size distribution, zeta potential, vesicle deformability, drug release, skin permeation, retention, texture, antioxidant and cytotoxicity activities. The optimized formulation showed a vesicle size of 119.20 ± 15.67 nm with a polydispersity index (PDI) of 0.145 ± 0.02, the zeta potential of −27 ± 5.12 mV and entrapment efficiency of 76.42 ± 9.98%. The optimized PLM-loaded GM formulation was transformed into a pre-formed gel which was prepared using Carbopol 934 polymer. The drug diffusion fluxes of CL gel and GM-loaded gel were 23.31 ± 6.0 and 79.43 ± 12.43 µg/cm2/h, respectively. The result of texture analysis revealed the adequate hardness, cohesiveness, consistency, and viscosity of the developed GM-loaded gel compared to CL gel. The confocal images showed that glycerosomal gel has deeper skin layer penetration as compared to the control solution. GM-loaded gel treated rat skin showed significantly (p < 0.05) higher drug accumulation in the dermis, higher cytotoxicity and higher antioxidant activity as compared to CL gel and PLM suspension. Thus, findings revealed that novel GM-loaded gel could be potential carriers for therapeutic intervention in skin cancer.
Ex-Situ Evaluation of Commercial Polymer Membranes for Vanadium Redox Flow Batteries (VRFBs)
Authors: Zhao Nana , Riley Harry , Song Chaojie , Jiang Zhengming , Tsay Keh-Chyun , Neagu Roberto , Shi Zhiqing
Published: March 2021

Polymer membranes play a vital role in vanadium redox flow batteries (VRFBs), acting as a separator between the two compartments, an electronic insulator for maintaining electrical neutrality of the cell, and an ionic conductor for allowing the transport of ionic charge carriers. It is a major influencer of VRFB performance, but also identified as one of the major factors limiting the large-scale implementation of VRFB technology in energy storage applications due to its cost and durability. In this work, five (5) high-priority characteristics of membranes related to VRFB performance were selected as major considerable factors for membrane screening before in-situ testing. Eight (8) state-of-the-art of commercially available ion exchange membranes (IEMs) were specifically selected, evaluated and compared by a set of ex-situ assessment approaches to determine the possibility of the membranes applied for VRFB. The results recommend perfluorosulfonic acid (PFSA) membranes and hydrocarbon anion exchange membranes (AEMs) as the candidates for further in-situ testing, while one hydrocarbon cation exchange membrane (CEM) is not recommended for VRFB application due to its relatively high VO2+ ion crossover and low mechanical stability during/after the chemical stability test. This work could provide VRFB researchers and industry a valuable reference for selecting the polymer membrane materials before VRFB in-situ testing.
Chemical Recycling of WEEE Plastics—Production of High Purity Monocyclic Aromatic Chemicals
Authors: Rieger Tobias , Oey Jessen C. , Palchyk Volodymyr , Hofmann Alexander , Franke Matthias , Hornung Andreas
Published: March 2021

More than 200 kg real waste electrical and electronic equipment (WEEE) shredder residues from a German dismantling plant were treated at 650 °C in a demonstration scale thermochemical conversion plant. The focus within this work was the generation, purification, and analysis of pyrolysis oil. Subsequent filtration and fractional distillation were combined to yield basic chemicals in high purity. By means of fractional distillation, pure monocyclic aromatic fractions containing benzene, toluene, ethylbenzene, and xylene (BTEX aromatics) as well as styrene and α-methyl styrene were isolated for chemical recycling. Mass balances were determined, and gas chromatography–mass spectrometry (GC-MS) as well as energy dispersive X-ray fluorescence (EDXRF) measurements provided data on the purity and halogen content of each fraction. This work shows that thermochemical conversion and the subsequent refining by fractional distillation is capable of recycling WEEE shredder residues, producing pure BTEX and other monocyclic aromatic fractions. A significant decrease of halogen content (up to 99%) was achieved with the applied methods.
The Reattachment Process of a Lifted Jet Diffusion Flame by Repetitive DC Pulse Discharges
Authors: Chao Yei-Chin , Chang Tzu-Wei , Chan Yu-Pei
Published: March 2021

On research of plasma assisted combustion, effects of electric and plasma discharges in DC, AC and pulse forms on reattachment of a lifted flame have attracted extensive attention. However, the detailed plasma assisted reattachment process and mechanism and roles of induced corona discharge and corona-induced ozone on the reattachment process are still unclear and undocumented. The forced reattachment process of a lifted diffusion jet flame by repetitive DC electric pulse discharges was experimentally investigated in this study using high-speed flame imaging, conditioned particle image velocimetry (PIV), and planar ozone concentration imaging. The forced reattachment process can be divided into three stages in sequence: ionic wind prior to corona initiation, corona initiation, and corona enhancement propagation. The conditioned PIV results showed that the instantaneous flame base propagation velocity is sufficiently enhanced beyond the laminar burning velocity for high pulse-repetition-frequency (PRF) cases at the instant of pulse discharge (on pulse) due to the enhanced oxidation of the corona induced ozone. By observing the dynamic flame-base behavior and evolution characteristics of the short-lived corona induced ozone for various PRFs, the novel forced reattachment process and mechanism of a lifted jet flame induced by repetitive DC electric pulse discharges is proposed.
Does Science Need God? A Theistic Argument from Science
Authors: Hołda Miłosz , Wojtysiak Jacek
Published: March 2021

In our paper, we put forward an argument for the existence of God that starts with a description of the goal of science. The fact that science approximates perfect knowledge opens the problem of its status. We proceed to three resolutions of the problem: perfect knowledge is only a kind of fictional idealization; it will be reached by humanity in the future; it is God’s knowledge. We point out the weaknesses of the first two options. Next, we go on to draw the conclusion that it is hardly possible to describe the goal of science without some theistic or near-theistic concepts.
Give Them Christ: Native Agency in the Evangelization of Puerto Rico, 1900 to 1917
Authors: Santiago-Vendrell Angel
Published: March 2021

The scholarship on the history of Protestant missions to Puerto Rico after the Spanish American War of 1898 emphasizes the Americanizing tendencies of the missionaries in the construction of the new Puerto Rican. There is no doubt that the main missionary motif during the 1890s was indeed civilization. Even though the Americanizing motif was part of the evangelistic efforts of some missionaries, new evidence shows that a minority of missionaries, among them Presbyterians James A. McAllister and Judson Underwood, had a clear vision of indigenization/contextualization for the emerging church based on language (Spanish) and culture (Puerto Rican). The spread of Christianity was successful not only because of the missionaries but also because native agents took up the task of evangelizing their own people; they were not passive spectators but active agents translating and processing the message of the gospel to fulfill their own people’s needs based on their own individual cultural assumptions. This article problematizes the past divisions of such evangelizing activities between the history of Christianity, mission history, and theology by analyzing the native ministries of Adela Sousa (a Bible woman) and Miguel Martinez in light of the teachings of the American missionaries. The investigation claims that because of Puerto Rican agents’ roles in the process of evangelization, a new fusion between the history of Christianity, mission history, and theology emerged as soon as new converts embraced and began to preach the gospel.
Development and Validation of a Faith Scale for Young Children
Authors: Kim Sungwon
Published: March 2021

The purpose of this study is to develop and validate a faith scale for young children. Data were collected from 424 young children, who had not yet entered elementary school, with their parents rating their faith level. Sixty-five preliminary questions were formulated under three domains―knowing, loving, and living—that were based on existing studies related to faith. The questions were reduced to 40 through a content validity test conducted by a seven-member panel. These questions were subsequently refined through pilot study, main survey, and statistical analysis. After exploratory and confirmatory factor analysis, the scale was finalized, comprising 25 questions that can be categorized into three factors: confessional faith life, missional life, and distinctive life. This scale is expected to measure early childhood faith and prove the effectiveness of Christian education programs on a young child’s faith development.
Evangelical and Catholic Timespace in Work: An Argument against a Homeless Eschatology
Authors: Ledstam Maria
Published: March 2021

This article engages with how religion and economy relate to each other in faith-based businesses. It also elaborates on a recurrent idea in theological literature that reflections on different visions of time can advance theological analyses of the relationship between Christianity and capitalism. More specifically, this article brings results from an ethnographic study of two faith-based businesses into conversation with the ethicist Luke Bretherton’s presentation of different understandings of the relationship between Christianity and capitalism. Using Theodore Schatzki’s theory of timespace, the article examines how time and space are constituted in two small faith-based businesses that are part of the two networks Business as Mission (evangelical) and Economy of Communion (catholic) and how the different timespaces affect the religious-economic configurations in the two cases and with what moral implications. The overall findings suggest that the timespace in the Catholic business was characterized by struggling caused by a tension between certain ideals on how religion and economy should relate to each other on the one hand and how the practice evolved on the other hand. Furthermore, the timespace in the evangelical business was characterized by confidence, caused by the business having a rather distinct and achievable goal when it came to how they wanted to be different and how religion should relate to economy. There are, however, nuances and important resemblances between the cases that cannot be explained by the businesses’ confessional and theological affiliations. Rather, there seems to be something about the phenomenon of tension-filled and confident faith-based businesses that causes a drive in the practices towards the common good. After mapping the results of the empirical study, I discuss some contributions that I argue this study brings to Bretherton’s presentation of the relationship between Christianity and capitalism.
Sentinel-1 A-DInSAR Approaches to Map and Monitor Ground Displacements
Authors: Krishnakumar Vrinda , Qiu Zhiwei , Monserrat Oriol , Barra Anna , López-Vinielles Juan , Reyes-Carmona Cristina , Gao Qi , Cuevas-González Maria , Palamà Riccardo , Crippa Bruno , Gili Jose Antonio
Published: March 2021

Persistent scatterer interferometry (PSI) is a group of advanced interferometric synthetic aperture radar (SAR) techniques used to measure and monitor terrain deformation. Sentinel-1 has improved the data acquisition throughout and, compared to previous sensors, increased considerably the differential interferometric SAR (DInSAR) and PSI deformation monitoring potential. The low density of persistent scatterer (PS) in non-urban areas is a critical issue in DInSAR and has inspired the development of alternative approaches and refinement of the PS chains. This paper proposes two different and complementary data-driven procedures to obtain terrain deformation maps. These approaches aim to exploit Sentinel-1 highly coherent interferograms and their short revisit time. The first approach, called direct integration (DI), aims at providing a very fast and straightforward approach to screen-wide areas and easily detects active areas. This approach fully exploits the coherent interferograms from consecutive images provided by Sentinel-1, resulting in a very high sampling density. However, it lacks robustness and its usability lays on the operator experience. The second method, called persistent scatterer interferometry geomatics (PSIG) short temporal baseline, provides a constrained application of the PSIG chain, the CTTC approach to the PSI. It uses short temporal baseline interferograms and does not assume any deformation model for point selection. It is also quite a straightforward approach, which improves the performances of the standard PSIG approach, increasing the PS density and providing robust measurements. The effectiveness of the approaches is illustrated through analyses performed on different test sites.
Interannual Climate Variability in the West Antarctic Peninsula under Austral Summer Conditions
Authors: Santamaría-del-Ángel Eduardo , Cañon-Páez Mary-Luz , Sebastiá-Frasquet Maria-Teresa , González-Silvera Adriana , Gutierrez Angelica-L. , Aguilar-Maldonado Jesús-A. , López-Calderón Jorge , Camacho-Ibar Víctor , Franco-Herrera Andrés , Castillo-Ramírez Alejandra
Published: March 2021

This study aimed to describe the interannual climate variability in the West Antarctic Peninsula (WAP) under austral summer conditions. Time series of January sea-surface temperature (SST) at 1 km spatial resolution from satellite-based multi-sensor data from Moderate Resolution Imaging Spectrometer (MODIS) Terra, MODIS Aqua, and Visible Infrared Imager Radiometer Suite (VIIRS) were compiled between 2001 and 2020 at localities near the Gerlache Strait and the Carlini, Palmer, and Rothera research stations. The results revealed a well-marked spatial-temporal variability in SST at the WAP, with a one-year warm episode followed by a five-year cold episode. Warm waters (SST > 0 °C) reach the coast during warm episodes but remain far from the shore during cold episodes. This behavior of warm waters may be related to the regional variability of the Antarctic Circumpolar Current, particularly when the South Polar Front (carrying warm waters) reaches the WAP coast. The WAP can be divided into two zones representing two distinct ecoregions: the northern zone (including the Carlini and Gerlache stations) corresponds to the South Shetland Islands ecoregion, and the southern zone (including the Palmer and Rothera stations) corresponds to the Antarctic Peninsula ecoregion. The Gerlache Strait is likely situated on the border between the two ecoregions but under a greater influence of the northern zone. Our data showed that the Southern Annular Mode (SAM) is the primary driver of SST variability, while the El Niño Southern Oscillation (ENSO) plays a secondary role. However, further studies are needed to better understand regional climate variability in the WAP and its relation with SAM and ENSO; such studies should use an index that adequately describes the ENSO in these latitudes and addresses the limitations of the databases used for this purpose. Multi-sensor data are useful in describing the complex climate variability resulting from the combination of local and regional processes that elicit different responses across the WAP. It is also essential to continue improving SST approximations at high latitudes.
Drought Events over the Amazon River Basin (1993–2019) as Detected by the Climate-Driven Total Water Storage Change
Authors: Tian Kunjun , Wang Zhengtao , Li Fupeng , Gao Yu , Xiao Yang , Liu Cong
Published: March 2021

The Gravity Recovery and Climate Experiment (GRACE) mission has measured total water storage change (TWSC) and interpreted drought patterns in an unparalleled way since 2002. Nevertheless, there are few sources that can be used to understand drought patterns prior to the GRACE era. In this study, we extended the gridded GRACE TWSC to 1993 by combining principal component analysis (PCA), least square (LS) fitting, and multiple linear regression (MLR) methods using climate variables as input drivers. We used the extended (climate-driven) TWSC to interpret drought patterns (1993–2019) over the Amazon basin. Results showed that, in the Amazon area with the resolution of 0.5°, GRACE, GRACE follow on, and Swarm had correlation coefficients of 0.95, 0.92, and 0.77 compared with climate-driven TWSCS, respectively. The drought patterns assessed by the climate-driven TWSC were consistent with those interpreted by the Palmer Drought Severity Index and GRACE TWSC. We also found that the 1998 and 2016 drought events in the Amazon, both induced by strong El Niño events, showed similar drought patterns. This study provides a new perspective for interpreting long-term drought patterns prior to the GRACE period.
An Oil Well Dataset Derived from Satellite-Based Remote Sensing
Authors: Wang Zhibao , Bai Lu , Song Guangfu , Zhang Jie , Tao Jinhua , Mulvenna Maurice D. , Bond Raymond R. , Chen Liangfu
Published: March 2021

Estimation of the number and geo-location of oil wells is important for policy holders considering their impact on energy resource planning. With the recent development in optical remote sensing, it is possible to identify oil wells from satellite images. Moreover, the recent advancement in deep learning frameworks for object detection in remote sensing makes it possible to automatically detect oil wells from remote sensing images. In this paper, we collected a dataset named Northeast Petroleum University–Oil Well Object Detection Version 1.0 (NEPU–OWOD V1.0) based on high-resolution remote sensing images from Google Earth Imagery. Our database includes 1192 oil wells in 432 images from Daqing City, which has the largest oilfield in China. In this study, we compared nine different state-of-the-art deep learning models based on algorithms for object detection from optical remote sensing images. Experimental results show that the state-of-the-art deep learning models achieve high precision on our collected dataset, which demonstrate the great potential for oil well detection in remote sensing.
Assessing Irrigation Water Use with Remote Sensing-Based Soil Water Balance at an Irrigation Scheme Level in a Semi-Arid Region of Morocco
Authors: Kharrou Mohamed Hakim , Simonneaux Vincent , Er-Raki Salah , Le Page Michel , Khabba Saïd , Chehbouni Abdelghani
Published: March 2021

This study aims to evaluate a remote sensing-based approach to allow estimation of the temporal and spatial distribution of crop evapotranspiration (ET) and irrigation water requirements over irrigated areas in semi-arid regions. The method is based on the daily step FAO-56 Soil Water Balance model combined with a time series of basal crop coefficients and the fractional vegetation cover derived from high-resolution satellite Normalized Difference Vegetation Index (NDVI) imagery. The model was first calibrated and validated at plot scale using ET measured by eddy-covariance systems over wheat fields and olive orchards representing the main crops grown in the study area of the Haouz plain (central Morocco). The results showed that the model provided good estimates of ET for wheat and olive trees with a root mean square error (RMSE) of about 0.56 and 0.54 mm/day respectively. The model was then used to compare remotely sensed estimates of irrigation requirements (RS-IWR) and irrigation water supplied (WS) at plot scale over an irrigation district in the Haouz plain through three growing seasons. The comparison indicated a large spatio-temporal variability in irrigation water demands and supplies; the median values of WS and RS-IWR were 130 (175), 117 (175) and 118 (112) mm respectively in the 2002–2003, 2005–2006 and 2008–2009 seasons. This could be attributed to inadequate irrigation supply and/or to farmers’ socio-economic considerations and management practices. The findings demonstrate the potential for irrigation managers to use remote sensing-based models to monitor irrigation water usage for efficient and sustainable use of water resources.
Assessing the Effects of Land Cover Land Use Change on Precipitation Dynamics in Guangdong–Hong Kong–Macao Greater Bay Area from 2001 to 2019
Authors: Wang Xinghan , Cong Peitong , Jin Yuhao , Jia Xichun , Wang Junshu , Han Yuxing
Published: March 2021

The change of spatial and temporal distribution of precipitation has an important impact on urban water security. The effect of land cover land use change (LCLUC) on the spatial and temporal distribution of precipitation needs to be further studied. In this study, transfer matrix, standard deviation ellipse and spatial autocorrelation analysis techniques were used. Based on the data of land cover land use and precipitation, this paper analyzed the land cover land use change and its influence on the spatial and temporal distribution pattern of precipitation in the Guangdong–Hong Kong–Macao Greater Bay Area (GBA). The results showed that from 2001 to 2019, the area of cropland, water, barren, forest/grassland in the GBA decreased by 44.03%, 8.05%, 50.22%, 0.43%, respectively, and the area of construction land increased by 20.05%. The precipitation in the GBA was mainly concentrated in spring and summer, and the precipitation in spring tended to increase gradually, while the precipitation in summer tended to decrease gradually, while the precipitation in autumn and winter has no obvious change. It was found that with the change of land cover land use, the spatial distribution of precipitation also changed. Especially in the areas where the change of construction land was concentrated, the spatial distribution of precipitation changed most obviously.
A Model to Estimate Leaf Area Index in Loblolly Pine Plantations Using Landsat 5 and 7 Images
Authors: Kinane Stephen M. , Montes Cristian R. , Albaugh Timothy J. , Mishra Deepak R.
Published: March 2021

Vegetation indices calculated from remotely sensed satellite imagery are commonly used within empirically derived models to estimate leaf area index in loblolly pine plantations in the southeastern United States. The data used to parameterize the models typically come with observation errors, resulting in biased parameters. The objective of this study was to quantify and reduce the effects of observation errors on a leaf area index (LAI) estimation model using imagery from Landsat 5 TM and 7 ETM+ and over 1500 multitemporal measurements from a Li-Cor 2000 Plant Canopy Analyzer. Study data comes from a 16 quarter 1 ha plot with 1667 trees per hectare (2 m × 3 m spacing) fertilization and irrigation research site with re-measurements taken between 1992 and 2004. Using error-in-variable methods, we evaluated multiple vegetation indices, calculated errors associated with their observations, and corrected for them in the modeling process. We found that the normalized difference moisture index provided the best correlation with below canopy LAI measurements (76.4%). A nonlinear model that accounts for the nutritional status of the stand was found to provide the best estimates of LAI, with a root mean square error of 0.418. The analysis in this research provides a more extensive evaluation of common vegetation indices used to estimate LAI in loblolly pine plantations and a modeling framework that extends beyond the typical linear model. The proposed model provides a simple to use form allowing forest practitioners to evaluate LAI development and its uncertainty in historic pine plantations in a spatial and temporal context.