bluuebunny/crossref_metadata_2025 huggingface dataset:
Complications after Thermal Ablation of Hepatocellular Carcinoma and Liver Metastases: Imaging Findings
Authors: De Muzio Federica , Cutolo Carmen , Dell’Aversana Federica , Grassi Francesca , Ravo Ludovica , Ferrante Marilina , Danti Ginevra , Flammia Federica , Simonetti Igino , Palumbo Pierpaolo , Bruno Federico , Pierpaoli Luca , Fusco Roberta , Giovagnoni Andrea , Miele Vittorio , Barile Antonio , Granata Vincenza
Published: May 2022

Tumour ablation is a strategy of treatment of hepatic tumours in patients with small hepatocellular carcinoma (HCC) (<3 cm) or in patients unfit for surgical resection. Moreover, tumor ablation can be used as an adjuvant therapy or may be used in association with resection in case of patients with poor functional liver disease. These types of treatment usually could be performed percutaneously under image guidance. The most clinically verified and used ablation modalities are Radiofrequency Ablation (RFA) and microwave ablation (MWA). However, despite both of them are considered minimally invasive techniques, they could be related to post-procedural complications. The International Working Group on Image-Guided Tumor and the Society of Interventional Radiology (SIR) identified major and minor post-ablative complications. Major complications, as vascular complications, occur in 2.2% to 3.1% of cases and include all the high risk pathological conditions which could increase the level of care or result in hospital admission or substantially prolonged hospital stay (SIR classifications C–E). Minor complications, as biliary complications, occur in 5% to 8.9% and include self-limiting conditions that are considered to be of low risk for the patient’s outcome. The purpose of this review is to summarise the main pathological ultrasound (US) and Computed Tomography (CT) findings, that may arise after ablative treatment. To simplify the analysis, the pathological pictures are divided according to the site of damage into vascular, biliary and extrahepatic complications.
Comparative Evaluation of the Effectiveness of a Combination of Absorbable Gelatin Sponge and Calendula officinalis with Absorbable Gelatin Sponge Used Alone as a Hemostatic Agent—An In-Vitro Study
Authors: Ayyanahalli Matta Bharath Kumar , Kumar Santhosh , Mehta Chetan Hasmukh , Nayak Usha Yogendra , Rodriguez Patricia Garcia
Published: May 2022

Excessive bleeding can complicate surgical intervention; this could be managed using an effective hemostatic agent that provides immediate and early bleeding control. Gelatin sponge and Calendula officinalis have been proven to have good hemostatic properties. The present In-vitro study analyzed the cytotoxicity and hemostatic properties of gelatin sponge and Calendula officinalis. The cytotoxic concentration/effective concentration of Calendula officinalis was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The drug release was determined using a vertical Franz diffusion cell apparatus; solid-state characterization was assessed using Fourier-transform infrared spectroscopy (FTIR) and a differential scanning calorimeter (DSC). The MTT assay showed 7% Calendula officinalis to be cytocompatible, and there was an increase in cell proliferation. When the 7% Calendula officinalis was loaded into the sponge, it was compatible, and the drug content was found to be 56.28 ± 13.84%. The time taken for the blood clot formation was measured using the Lee–White method. The gelatin sponge’s time for clot formation was 161.70 ± 3.11 s, and the Calendula officinalis loaded gelatin sponge’s time for clot formation was 158.75 ± 4.60 s. Hence, it could be concluded that when Calendula officinalis is incorporated into a gelatin sponge, it shows material compatibility and cytocompatibility, reduces the time for clot formation, and could be used as an alternative to other hemostatic agents.
Entropy and the Experience of Heat
Authors: Fuchs Hans , D’Anna Michele , Corni Federico
Published: May 2022

We discuss how to construct a direct and experientially natural path to entropy as a extensive quantity of a macroscopic theory of thermal systems and processes. The scientific aspects of this approach are based upon continuum thermodynamics. We ask what the roots of an experientially natural approach might be—to this end we investigate and describe in some detail (a) how humans experience and conceptualize an extensive thermal quantity (i.e., an amount of heat), and (b) how this concept evolved during the early development of the science of thermal phenomena (beginning with the Experimenters of the Accademia del Cimento and ending with Sadi Carnot). We show that a direct approach to entropy, as the extensive quantity of models of thermal systems and processes, is possible and how it can be applied to the teaching of thermodynamics for various audiences.
Hybrid Feature Extraction Model to Categorize Student Attention Pattern and Its Relationship with Learning
Authors: Poudyal Sujan , Mohammadi-Aragh Mahnas J. , Ball John E.
Published: May 2022

The increase of instructional technology, e-learning resources, and online courses has created opportunities for data mining and learning analytics in the pedagogical domain. A large amount of data is obtained from this domain that can be analyzed and interpreted so that educators can understand students’ attention. In a classroom where students have their own computers in front of them, it is important for instructors to understand whether students are paying attention. We collected on- and off-task data to analyze the attention behaviors of students. Educational data mining extracts hidden information from educational records, and we are using it to classify student attention patterns. A hybrid method is used to combine various techniques like classifications, regressions, or feature extraction. In our work, we combined two feature extraction techniques: principal component analysis and linear discriminant analysis. Extracted features are used by a linear and kernel support vector machine (SVM) to classify attention patterns. Classification results are compared with linear and kernel SVM. Our hybrid method achieved the best results in terms of accuracy, precision, recall, F1, and kappa. Also, we correlated attention with learning. Here, learning corresponds to tests and a final course grade. For determining the correlation between grades and attention, Pearson’s correlation coefficient and p-value were used.
Run-Time Reconfiguration Strategy and Implementation of Time-Triggered Networks
Authors: Li Ji , Xiong Huagang , Li Qiao , Xiong Feng , Feng Jiaying
Published: May 2022

Time-triggered networks are deployed in avionics and astronautics because they provide deterministic and low-latency communications. Remapping of partitions and the applications that reside in them that are executing on the failed core and the resulting re-routing and re-scheduling are conducted when a permanent end-system core failure occurs and local resources are insufficient. We present a network-wide reconfiguration strategy as well as an implementation scheme, and propose an Integer Linear Programming based joint mapping, routing, and scheduling reconfiguration method (JILP) for global reconfiguration. Based on scheduling compatibility, a novel heuristic algorithm (SCA) for mapping and routing is proposed to reduce the reconfiguration time. Experimentally, JILP achieved a higher success rate compared to mapping-then-routing-and-scheduling algorithms. In addition, relative to JILP, SCA/ILP was 50-fold faster and with a minimal impact on reconfiguration success rate. SCA achieved a higher reconfiguration success rate compared to shortest path routing and load-balanced routing. In addition, scheduling compatibility plays a guiding role in ILP-based optimization objectives and ‘reconfigurable depth’, which is a metric proposed in this paper for the determination of the reconfiguration potential of a TT network.
Influence of Area and Volume Effect on Dielectric Behaviour of the Mineral Oil-Based Nanofluids
Authors: Khan Suhaib Ahmad , Tariq Mohd , Khan Asfar Ali , Alamri Basem , Mihet-Popa Lucian
Published: May 2022

Transformer oil is conventionally used as an insulating liquid for the purpose of insulation and cooling in power transformers. The rise in the power demand has put stress on the existing insulation system used for power transmission. Nanotechnology provides an advanced approach to upgrade the conventional insulation system by producing nano-oil with enhanced dielectric characteristics. The aim of the study is to present the influence of area volume effect on the dielectric performance of mineral oil and its nanofluids. In this paper, nanofluids are prepared by dispersing two different concentrations of SiO2 nanoparticles in base transformer oil using a two-step method. The effect of area and volume is investigated on nanofluids in the laboratory using coaxial electrode configurations under different test conditions. The AC breakdown voltage and maximum electric stress is determined for the pure oil and nanofluids. The results show that the addition of SiO2 nanoparticles significantly improves the dielectric characteristics of transformer oil. Moreover, the breakdown phenomenon is also discussed to analyze the effect of nanoparticle, stressed area, and stressed volume on the dielectric strength of insulating oil. Nanofluids could be an alternative to mineral oil.
Effect of Gaskets Geometry on the Performance of a Reverse Electrodialysis Cell
Authors: Sandoval-Sánchez Elier , De la Cruz-Barragán Ziomara , Miranda-Hernández Margarita , Mendoza Edgar
Published: May 2022

Salinity gradient energy (SGE) allows the difference in salt concentration in two volumes of water to be harnessed and transformed into clean energy. The most advanced SGE technology is reverse electrodialysis (RED) cells. Recent studies have focused on ways to optimize the flow distribution in the compartments containing the water, for which it is necessary to consider the characteristics of the solutions, the cell dimensions, the operating conditions, as well as their influence on the hydrodynamics and mass transport in the system. In this study, two spacers with different gasket geometry were designed, fabricated, and compared experimentally through voltage and current measurements. The power output was computed, obtaining a maximum power density of 0.14 W/m2. Results show that the geometry of the cell components directly influences the physicochemical principles governing the RED process and is closely related to the cell output parameters. In turn, it is possible to increase the performance of a RED cell by optimizing the gasket geometry by reducing dead zones.
Research on Noise Suppression Technology of Marine Optical Fiber Towed Streamer Seismic Data Based on ResUNet
Authors: Qian Hongfei , Wang Xiangchun , Chen Xuelei , Yang Zhu
Published: May 2022

Optical fiber seismic exploration technology has been widely used in marine oil and gas hydrate exploration due to its wide frequency band and high sensitivity. However, there are more types of noise in the collected data by optical fiber hydrophone than by a conventional piezoelectric seismic exploration system. Considering that the conventional denoising method is time-consuming, this paper proposes a convolutional neural network (CNN) and a ResUNet network based on deep learning to suppress the noises. ResUNet is improved on the basis of CNN; it is composed of a feature extraction part, a feature reconstruction part and a residual block. Both CNN and ResUNet networks achieved obvious denoising effects on optical fiber towed streamer seismic data and improved the signal-to-noise ratio of data effectively. The ResUNet network has better denoising effects than CNN, even better than conventional denoising methods. The ResUNet network can solve the problem of gradient disappearance caused by network deepening; it recovered edge data well, and it has high efficiency compared with conventional denoising methods. Two evaluation indexes, relative error (RE) and similarity structure degree (SSIM), were introduced to compare the denoising effect of the ResUNet network with that of CNN. The experimental results showed that the performance of the ResUNet network in these two aspects is better than that of CNN.
Mechanical Response of Geopolymer Foams to Heating—Managing Coal Gangue in Fire-Resistant Materials Technology
Authors: Sitarz Mateusz , Figiela Beata , Łach Michał , Korniejenko Kinga , Mróz Katarzyna , Castro-Gomes João , Hager Izabela
Published: May 2022

Two geopolymer foams were prepared from a thermally activated coal gangue containing kaolinite. As the foaming agent, aluminium powder and 36% hydrogen peroxide were used to obtain two levels of porosity. The materials’ high temperature performances were investigated: tensile and compressive strength evolution with temperature. This study shows that the mechanical performances of developed geopolymer foams are similar to foam concrete of the same apparent density. The geopolymer foams from coal gangue present stable mechanical performances up to 600 °C. When the glass transition temperature is achieved, sintering occurs and mechanical performance increases. SEM observations confirm the glass transition and densification of the matrix at temperatures above 800 °C. Moreover, the XRD measurements revealed a high amount of mullite that forms at 1000 °C that explained the observed strength increase. The synthesis of good-quality geopolymer foams from coal gangue and its application as a thermal barrier is feasible. The constant level of porosity and its stable character in the range of temperatures 20–1000 °C ensures stable thermal insulation parameters with increasing temperature, which is extremely important for fire protection.
Research on VFTO Identification of GIS Based on Wavelet Transform and Singular Value Decomposition
Authors: Xiao Gang , Rong Quansen , Yang Miaoran , Xiao Peng , Chen Qihong , Fan Junzhe , Guo Haoran , Wang Haonan
Published: May 2022

The accurate identification of Very Fast Transient Overvoltage (VFTO) is the key of overvoltage control in modern smart grids. In order to accurately identify VFTO generated by the operation of a disconnector in Gas Insulated Substation (GIS), a VFTO identification method based on Wavelet Transform (WT) and Singular Value Decomposition (SVD) is proposed. The simulation model of VFTO is established in ATP-EMTP software first, and then wavelet decomposition is used in MATLAB software for VFTO simulation of the waveform from the ATP-EMTP software. Then, the feature matrix is composed of the coefficients of each frequency layer of the wavelet. The SVD is used to decompose the feature matrix, and finally the characteristic parameters of the VFTO are obtained. The simulation results in Matlab software indicate that the characteristic parameters of VFTO have an obvious difference compared with those of power frequency AC voltage, especially in the load-side, which verifies the effectiveness of the VFTO identification method based on WT and SVD proposed in this paper.
The Complete Mitochondrial Genome of Stichopus naso (Aspidochirotida: Stichopodidae: Stichopus) and Its Phylogenetic Position
Authors: Li Zhuobo , Ma Bo , Li Xiaomin , Lv Ying , Jiang Xiao , Ren Chunhua , Hu Chaoqun , Luo Peng
Published: May 2022

The mitochondrial genome is widely used to study the molecular evolution of and perform phylogenetic analyses on animals. In this study, the complete mitochondrial genome (mitogenome) of Stichopus naso was sequenced. The mitogenome was 16,239 bp in length and contained 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and 2 ribosomal RNA genes (rRNAs). The genome composition showed positive AT-skew (0.023) and negative GC-skew (−0.158). The order of the mitochondrial genes was consistent with those from the Stichopus and Isostichopus species, whereas it was different from those of other species of Aspidochirotida. The phylogenetic analysis, based on the nucleotide sequences of 13 PCGs through the methods of Bayesian inference (BI) and maximum likelihood (ML), indicated that S. naso has close relationships with S. horrens and S. monotuberculatus, and belongs to a member of Stichopodidae. Our study provides a reference mitogenome for further molecular evolution studies and phylogenetic research on sea cucumbers.
Knee Kinetics and Kinematics of Young Asymptomatic Participants during Single-Leg Weight-Bearing Tasks: Task and Sex Comparison of a Cross-Sectional Study
Authors: Bellizzi Gustavo Luís , Will-Lemos Tenysson , Resende Renan Alves , Cervi Ana Cristina Corrêa , Santiago Paulo Roberto Pereira , Fernández-de-las-Peñas César , Bevilaqua-Grossi Débora , Florencio Lidiane Lima
Published: May 2022

This cross-sectional study aimed to describe and compare kinetic and kinematic variables of the knee joint during stair descent, single-leg step down, and single-leg squat tasks. It also aimed to investigate potential sex difference during the tasks. Thirty young asymptomatic individuals (15 males, 15 females) were assessed during the performance of single-leg weight-bearing tasks. The kinetic and kinematic data from the knee were evaluated at the peak knee moment and at peak knee flexion. Single-leg squat presented a higher peak knee moment (2.37 Nm/kg) and the greatest knee moment (1.91 Nm/kg) at knee peak angle in the frontal plane, but the lowest knee flexion (67°) than the other two tasks (p < 0.05). Additionally, the single-leg step down task presented a higher varus knee angle (5.70°) when compared to stair descent (3.71°) (p < 0.001). No substantial sex difference could be observed. In conclusion, in asymptomatic young individuals, single-leg squats presented the greatest demand in the frontal and sagittal planes. Single-leg step down demanded a greater angular displacement than stair descent in the frontal plane. We did not identify a significant difference among the sex and studied variables.
The Continued Impact of the COVID-19 Pandemic on Pediatric Obesity: A Commentary on the Return to a Healthy New “Normal”
Authors: Chaves Eileen , Reddy Sheethal D. , Cadieux Adelle , Tomasula Jessica , Reynolds Kimberly
Published: May 2022

Two years into this pandemic, mental health symptoms are more prevalent in children and adolescents, routine wellness visits have decreased, individuals and families are experiencing increased stress, and food and nutrition insecurity are on the rise. Pediatric overweight and obesity are yet another health condition that has been impacted by the pandemic. The current commentary aims to (a) summarize a variety of factors contributing to worsening obesity and healthy lifestyle choices in youth throughout the pandemic and to (b) provide recommendations for healthcare providers on navigating this challenge. Specific health behaviors, such as increased sedentary behavior, decreased physical activity, a change to families’ home-food environments, and an increase in sleep dysregulation have contributed to increased weight gain in children and adolescents. As uncertainty continues with the advent of various COVID-19 variants, it remains important to consider how the pandemic has impacted pediatric overweight and obesity.
Association between Fat Distribution and Chronic Low Back Pain among 10,606 Adults: Data from the Korean National Health and Nutrition Examination Survey
Authors: Oh Minji , Kim Jongyeon , Lee Suji , Lee Seunghoon , Lee Jae-Dong
Published: May 2022

Obesity is associated with chronic low back pain (CLBP), but the association between fat distribution and CLBP is unclear. This cross-sectional study evaluated the relationship using the Korean National Health and Nutrition Examination Survey data. A total of 10,606 adults (average age: 45.4, female: 57.1%) were included. We estimated the regional fat distribution, waist circumference, and body fat proportion, compared the values in people with and without CLBP, and stratified the estimates by sex and obesity status using a multivariable linear model. There were no statistically significant differences in the average waist circumference between the people with and without CLBP (p = 0.731) and the average fat proportion between those with and without CLBP (p = 0.731). The average regional fat distribution was significantly higher in the people with CLBP than in those without CLBP, in the upper limbs (11.4%, 95% confidence interval [CI]: [11.3, 11.5] vs. 11.2%, 95% CI: [11.1, 11.3], p < 0.05) and in the lower limbs (31.9%, 95% CI: [31.6, 32.2] vs. 31.4%, 95% CI: [31.2, 31.6], p < 0.01). More obvious among men, fat distribution in the lower limbs is higher than in people without obesity (p < 0.001). People with CLBP tend to have a higher fat distribution in the limbs than those without it and obese people with CLBP would need to reduce the fat in the lower limbs.
The Autism–Psychosis Continuum Conundrum: Exploring the Role of the Endocannabinoid System
Authors: Colizzi Marco , Bortoletto Riccardo , Costa Rosalia , Bhattacharyya Sagnik , Balestrieri Matteo
Published: May 2022

Evidence indicates shared physiopathological mechanisms between autism and psychosis. In this regard, the endocannabinoid system has been suggested to modulate neural circuits during the early stage of neurodevelopment, with implications for both autism and psychosis. Nevertheless, such potential common markers of disease have been investigated in both autism and psychosis spectrum disorders, without considering the conundrum of differentiating the two groups of conditions in terms of diagnosis and treatment. Here, we systematically review all human and animal studies examining the endocannabinoid system and its biobehavioral correlates in the association between autism and psychosis. Studies indicate overlapping biobehavioral aberrancies between autism and schizophrenia, subject to correction by modulation of the endocannabinoid system. In addition, common cannabinoid-based pharmacological strategies have been identified, exerting epigenetic effects across genes controlling neural mechanisms shared between autism and schizophrenia. Interestingly, a developmental and transgenerational trajectory between autism and schizophrenia is supported by evidence that exogenous alteration of the endocannabinoid system promotes progression to inheritable psychosis phenotypes in the context of biobehavioral autism vulnerability. However, evidence for a diametral association between autism and psychosis is scant. Several clinical implications follow from evidence of a developmental continuum between autism and psychosis as a function of the endocannabinoid system dysregulation.
Use of Tobacco and Nicotine Products among Young People in Denmark—Status in Single and Dual Use
Authors: Bast Lotus Sofie , Klitgaard Marie Borring , Kjeld Simone Gad , Jarlstrup Nanna Schneekloth , Christensen Anne Illemann
Published: May 2022

Lots of new tobacco or nicotine products are being launched, e.g., e-cigarettes and smokeless tobacco, which appeal especially to the youngest part of the population. For example, the use of smokeless tobacco among Danish youth rose from approx. 2% in 2010 to 9% in 2020. Hence, there is an urgent need to follow and intervene against youth tobacco or nicotine product use. This study explored the current use of cigarettes, e-cigarettes, heated tobacco, and smokeless tobacco among Danish 15- to 29-year-olds. Further, we examined the concurrent use of two products or more. We used a nationwide survey conducted among 15- to 29-year-olds in February and March 2020. Overall, approx. 35,700 individuals received the questionnaire of which 35.5% responded (n = 13,315). One out of five (20.1%) smoked cigarettes, half of them daily, the other half occasionally. About one in twenty (3.9%) used e-cigarettes (daily or occasionally), and more than one in three (31.6%) had tried e-cigarettes. The use of heated tobacco among Danish youth is still relatively limited (0.3%). In comparison, about 9% used smokeless tobacco (daily or occasionally). Overall, 27.0% stated that they use at least one type of tobacco or nicotine product, while 5.6% used more than one product. Monitoring tobacco-related behavior in youth provides extremely important information for, e.g., policymakers and health professionals.
Measuring User Engagement with a Socially Connected, Gamified Health Promotion Mobile App
Authors: White Becky K. , Burns Sharyn K. , Giglia Roslyn C. , Dhaliwal Satvinder S. , Scott Jane A.
Published: May 2022

Participant engagement is an important consideration in mHealth interventions and there are no standardised measurements available to guide researchers. This paper describes the engagement index customised for the Milk Man app, a mobile app designed to engage fathers with breastfeeding and parenting information. Participants were recruited from maternity hospitals in Perth, Western Australia. An engagement index with scores ranging from 0 to 100 was calculated. Kaplan Meier survival analysis was used to determine difference in duration of exclusive breastfeeding, and Pearson’s chi square analysis was conducted to investigate the association of engagement level with demographic characteristics and exclusive breastfeeding at 6 weeks. While overall, partners of participants who installed Milk Man were less likely to have ceased exclusive breastfeeding at any time point from birth to six weeks postpartum, this result was modest and of borderline significance (log rank test p = 0.052; Breslow p = 0.046; Tarone-Ware p = 0.049). The mean engagement score was 29.7% (range 1–80%), median 27.6%. Engagement level had no impact on duration of exclusive breastfeeding and demographic factors were not associated with engagement level. This research demonstrates a range of metrics that can be used to quantify participant engagement. However, more research is needed to identify ways of measuring effective engagement.
Chronic Microplastic Exposure and Cadmium Accumulation in Blue Crabs
Authors: Hernández-López María , Romero Diego
Published: May 2022

Aquatic ecosystems are severely threatened by the presence of a multitude of pollutants. In seas and oceans, the amount of plastics continues to increase and there is great concern about toxic element accumulation. Specifically, cadmium (Cd), a toxic metal, is highly relevant to public health safety due to its ability to accumulate in the internal tissues of crustaceans; likewise, microplastics (MPs) are emerging as pollutants capable of causing alterations in marine organisms. The aim of this study was thus to evaluate the accumulation and distribution of Cd in the tissue of blue crabs (Callinectes sapidus) chronically exposed to MPs (25 μg L−1). In total, 24 crabs were exposed in water for 118 days to 2 types of MPs (virgin and oxidised). During the final 21 days of the experiment, the crabs were fed with tuna liver, a viscera in which Cd accumulates (mean of 7.262 µg g−1). The presence of MPs caused no changes in Cd concentrations in either the haemolymph or tissues (hepatopancreas, gills, and muscles) of the crabs, although for oxidised MPs, there was a positive correlation between Cd concentrations in the hepatopancreas and muscles, a relevant finding for food safety.
Are Personal Resources and Perceived Stress Associated with Psychological Outcomes among Israeli Teachers during the Third COVID-19 Lockdown?
Authors: Shinan-Altman Shiri , Levkovich Inbar
Published: May 2022

Teachers’ psychological responses during a pandemic, such as COVID-19, play an important role in their adaptation to the new routine. This research aimed to explore the association between personal resources (sense of control, social support), perceived stress, and three psychological outcomes (resilience, depression, loneliness) among teachers during the third COVID-19 lockdown in Israel. A cross-sectional study was conducted among 208 teachers. Significant associations were found between perceived stress, resilience, depression, and loneliness. Sense of control was significantly associated with perceived social support. The research model was tested using Structural Equation Modeling. The model yielded appropriate indices of fit (χ2(10) = 10.31, χ2/df = 1.03, p = 0.413, NFI = 0.970, NNFI = 0.997, CFI = 0.999, RMSEA = 0.012, 95%CI RMSEA = 0.077), demonstrating that the model fits the data well. Findings suggest that in order to improve teachers’ psychological health during a virus outbreak, it is recommended to pay attention to their personal resources and perceived stress.
A New Nonlinear Photothermal Iterative Theory for Port-Wine Stain Detection
Authors: Cao Na , Liang Hongtao , Zhang Ruoyu , Li Yanhua , Cao Hui
Published: May 2022

The development of appropriate photothermal detection of skin diseases to meet complex clinical demands is an urgent challenge for the prevention and therapy of skin cancer. An extensive body of literature has ignored all high-order harmonics above the second order and their influences on low-order harmonics. In this paper, a new iterative numerical method is developed for solving the nonlinear thermal diffusion equation to improve nonlinear photothermal detection for the noninvasive assessment of the thickness of port-wine stain (PWS). First, based on the anatomical and structural properties of skin tissue of PWS, a nonlinear theoretical model for photothermal detection is established. Second, a corresponding nonlinear thermal diffusion equation is solved by using the new iterative numerical method and taking into account harmonics above the second-order and their effects on lower-order harmonics. Finally, the thickness and excitation light intensity of PWS samples are numerically simulated. The simulation results show that the numerical solution converges fasterand the physical meaning of the solution is clearerwith the new method than with the traditional perturbation method. The rate of change in each harmonic with the sample thickness for the new method is higher than that for the conventional perturbation method, suggesting that the proposed numerical method may provide greater detection sensitivity. The results of the study provide a theoretical basis for the clinical treatment of PWS.
Food Practice Lifestyles: Identification and Implications for Energy Sustainability
Authors: Giordono Leanne S. , Flora June , Zanocco Chad , Boudet Hilary
Published: May 2022

Food systems, including production, acquisition, preparation, and consumption, feature importantly in environmental sustainability, energy consumption and climate change. With predicted increases in food and water shortages associated with climate change, food-related lifestyle and behavioral changes are advocated as important mitigation and adaptation measures. Yet, reducing emissions from food systems is predicted to be one of our greatest challenges now and in the future. Traditional theories of environmental behavioral change often assume that individuals make “reasoned choices” that incorporate cost–benefit assessment, moral and normative concerns and affect/symbolic motives, yielding behavioral interventions that are often designed as informational or structural strategies. In contrast, some researchers recommend moving toward an approach that systematically examines the temporal organization of society with an eye toward understanding the patterns of social practices to better understand behaviors and develop more targeted and effective interventions. Our study follows on these recommendations with a study of food consumption “lifestyles” in the United States, using extant time use diary data from a nationally representative sample of Americans (n = 16,100) from 2014 to 2016. We use cluster analysis to identify unique groups based on temporal and locational eating patterns. We find evidence of six respondent clusters with distinct patterns of food consumption based on timing and location of eating, as well as individual and household characteristics. Factors associated with cluster membership include age, employment status, and marital status. We note the close connections between age and behaviors, suggesting that a life course scholarship approach may add valuable insight. Based on our findings, we identify opportunities for promoting sustainable energy use in the context of the transition to renewables, such as targeting energy-shifting and efficiency-improvement interventions based on group membership.
Phosphoproteomics Unravel HBV Triggered Rewiring of Host Phosphosignaling Events
Authors: Lim ZiJie , Mohd-Ismail Nur Khairiah Binte , Png Evelyn , Sze Ching Wooen , Lin Qifeng , Hong Wanjin , Lim Seng Gee , Tan Yee-Joo , Gunaratne Jayantha
Published: May 2022

Hepatitis B virus (HBV) infection persists as a major global health problem despite the availability of HBV vaccines for disease prevention. However, vaccination rates remains low in some regions of the world, driving the need for novel strategies to minimise infections and prevent disease progression. Thus, understanding of perturbed molecular signaling events during early phases of HBV infection is required. Phosphosignaling is known to be involved in the HBV infection processes, yet systems-level changes in phosphosignaling pathways in the host during infection remain unclear. To this end, we performed phosphoproteome profiling on HBV-infected HepG2-NTCP cells. Our results showed that HBV infection drastically altered the host phosphoproteome and its associated proteins, including kinases. Computational analysis of this phosphoproteome revealed dysregulation of the pathways involved in immune responses, cell cycle processes, and RNA processing during HBV infection. Kinase Substrate Enrichment Analysis (KSEA) identified the dysregulated activities of important kinases, including those from CMGC (CDK, MAPK, GSK, and CLK), AGC (protein kinase A, G, and C), and TK (Tyrosine Kinase) families. Of note, the inhibition of CLKs significantly reduced HBV infection in HepG2-NTCP cells. In all, our study unravelled the aberrated phosphosignaling pathways and the associated kinases, presenting potential entry points for developing novel therapeutic strategies for HBV treatment.
The Presence of Blood–Brain Barrier Modulates the Response to Magnesium Salts in Human Brain Organoids
Authors: Cazzaniga Alessandra , Fedele Giorgia , Castiglioni Sara , Maier Jeanette A.
Published: May 2022

Magnesium (Mg) is fundamental in the brain, where it regulates metabolism and neurotransmission and protects against neuroinflammation. To obtain insights into the molecular basis of Mg action in the brain, we investigated the effects of Mg in human brain organoids, a revolutionary 3D model to study neurobiology and neuropathology. In particular, brain organoids derived from human induced pluripotent stem cells were cultured in the presence or in the absence of an in vitro-generated blood–brain barrier (BBB), and then exposed to 1 or 5 mM concentrations of inorganic and organic Mg salts (Mg sulphate (MgSO4); Mg pidolate (MgPid)). We evaluated the modulation of NMDA and GABAergic receptors, and BDNF. Our data suggest that the presence of the BBB is essential for Mg to exert its effects on brain organoids, and that 5 mM of MgPid is more effective than MgSO4 in increasing the levels of GABA receptors and BDNF, and decreasing those of NMDA receptor. These results might illuminate novel pathways explaining the neuroprotective role of Mg.
Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
Authors: Zeng Zheng , Xie Yinhong , Li Li , Wang Huanran , Tan Jianying , Li Xia , Bian Qihao , Zhang Yu , Liu Tao , Weng Yajun , Chen Junying
Published: May 2022

Vascular stent service involves complex service environments and performance requirements, among which the histocompatibility of the stent could seriously affect the therapeutic effect. In the pathology of vascular disease, the thin fiber cap is easily ruptured, exposing the necrotic core below, and triggering a series of dangerous biochemical reactions. In contrast, the thin neointima, considered an essential structure growing on the stent, may evolve into vulnerable plaque structures due to lesions induced by the stent. Therefore, the reduction of necrosis around the stent below the thin neointima is indispensable. In this work, different cell model experiments suggested that the content of endogenous labile Zn positively correlated with cell injury. Zinquin-Zn fluorescence experiments and zinc ion channels research suggested that the change in the content of endogenous labile Zn in smooth muscle cells is affected by different stent coatings. The content of endogenous labile Zn in cells negatively correlated with cell viability. Animal experiments indirectly verified the increase in endogenous labile Zn by detecting the expression of Zn regulatory protein (metallothionein) in the necrotic tissues. Reducing the content of endogenous labile Zn may favor a reduction in smooth muscle cell injury and necrosis. This biochemical mechanism is effective in improving the therapeutic effect of vascular stents.
Cullin 3 Exon 9 Deletion in Familial Hyperkalemic Hypertension Impairs Cullin3-Ring-E3 Ligase (CRL3) Dynamic Regulation and Cycling
Authors: Kouranti Ilektra , Abdel Khalek Waed , Mazurkiewicz Stephani , Loisel-Ferreira Irmine , Gautreau Alexis M. , Pintard Lionel , Jeunemaitre Xavier , Clauser Eric
Published: May 2022

Cullin 3 (CUL3) is the scaffold of Cullin3 Ring E3-ligases (CRL3s), which use various BTB-adaptor proteins to ubiquitinate numerous substrates targeting their proteasomal degradation. CUL3 mutations, responsible for a severe form of familial hyperkalemia and hypertension (FHHt), all result in a deletion of exon 9 (amino-acids 403-459) (CUL3-∆9). Surprisingly, while CUL3-∆9 is hyperneddylated, a post-translational modification that typically activates CRL complexes, it is unable to ubiquitinate its substrates. In order to understand the mechanisms behind this loss-of function, we performed comparative label-free quantitative analyses of CUL3 and CUL3-∆9 interactome by mass spectrometry. It was observed that CUL3-∆9 interactions with COP9 and CAND1, both involved in CRL3 complexes’ dynamic assembly, were disrupted. These defects result in a reduction in the dynamic cycling of the CRL3 complexes, making the CRL3-∆9 complex an inactive BTB-adaptor trap, as demonstrated by SILAC experiments. Collectively, the data indicated that the hyperneddylated CUL3-∆9 protein is inactive as a consequence of several structural changes disrupting its dynamic interactions with key regulatory partners.
Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems
Authors: Sushko Ekaterina S. , Vnukova Natalia G. , Churilov Grigoriy N. , Kudryasheva Nadezhda S.
Published: May 2022

The Gd-containing metallofullerene derivatives are perspective magnetic resonance imaging contrast agents. We studied the bioeffects of a water-soluble fullerene derivative, gadolinium-endohedral fullerenol, with 40–42 oxygen groups (Gd@Fln). Bioluminescent cellular and enzymatic assays were applied to monitor toxicity and antioxidant activity of Gd@Fln in model solutions; bioluminescence was applied as a signaling physiological parameter. The Gd@Fln inhibited bioluminescence at high concentrations (>2·10−1 gL−1), revealing lower toxicity as compared to the previously studied fullerenols. Efficient activation of bioluminescence (up to almost 100%) and consumption of reactive oxygen species (ROS) in bacterial suspension were observed under low-concentration exposure to Gd@Fln (10−3–2·10−1 gL−1). Antioxidant capability of Gd@Fln was studied under conditions of model oxidative stress (i.e., solutions of model organic and inorganic oxidizers); antioxidant coefficients of Gd@Fln were determined at different concentrations and times of exposure. Contents of ROS were evaluated and correlations with toxicity/antioxidant coefficients were determined. The bioeffects of Gd@Fln were explained by hydrophobic interactions, electron affinity, and disturbing of ROS balance in the bioluminescence systems. The results contribute to understanding the molecular mechanism of “hormetic” cellular responses. Advantages of the bioluminescence assays to compare bioeffects of fullerenols based on their structural characteristics were demonstrated.
Discovery of Raf Family Is a Milestone in Deciphering the Ras-Mediated Intracellular Signaling Pathway
Authors: Zhao Jingtong , Luo Zhijun
Published: May 2022

The Ras-Raf-MEK-ERK signaling pathway, the first well-established MAPK pathway, plays essential roles in cell proliferation, survival, differentiation and development. It is activated in over 40% of human cancers owing to mutations of Ras, membrane receptor tyrosine kinases and other oncogenes. The Raf family consists of three isoforms, A-Raf, B-Raf and C-Raf. Since the first discovery of a truncated mutant of C-Raf as a transforming oncogene carried by a murine retrovirus, forty years of extensive studies have provided a wealth of information on the mechanisms underlying the activation, regulation and biological functions of the Raf family. However, the mechanisms by which activation of A-Raf and C-Raf is accomplished are still not completely understood. In contrast, B-Raf can be easily activated by binding of Ras-GTP, followed by cis-autophosphorylation of the activation loop, which accounts for the fact that this isoform is frequently mutated in many cancers, especially melanoma. The identification of oncogenic B-Raf mutations has led to accelerated drug development that targets Raf signaling in cancer. However, the effort has not proved as effective as anticipated, inasmuch as the mechanism of Raf activation involves multiple steps, factors and phosphorylation of different sites, as well as complex interactions between Raf isoforms. In this review, we will focus on the physiological complexity of the regulation of Raf kinases and their connection to the ERK phosphorylation cascade and then discuss the role of Raf in tumorigenesis and the clinical application of Raf inhibitors in the treatment of cancer.
Gradual Stress-Relaxation of Hydrogel Regulates Cell Spreading
Authors: Yu Wenting , Sun Wenxu , Chen Huiyan , Wang Juan , Xue Bin , Cao Yi
Published: May 2022

There is growing evidence that the mechanical properties of extracellular matrices (ECMs), including elasticity and stress-relaxation, greatly influence the function and form of the residing cells. However, the effects of elasticity and stress-relaxation are often correlated, making the study of the effect of stress-relaxation on cellular behaviors difficult. Here, we designed a hybrid network hydrogel with a controllable stress-relaxation gradient and a constant elasticity. The hydrogel is crosslinked by covalent bonds and dynamic peptide-metal ion coordination interactions. The stress-relaxation gradient is controlled by spatially controlling the coordination and covalent crosslinker ratios. The different parts of the hydrogel exhibit distinct stress-relaxation amplitudes but the have same stress-relaxation timescale. Based on this hydrogel, we investigate the influence of hydrogel stress-relaxation on cell spreading. Our results show that the spreading of cells is suppressed at an increasing stress-relaxation amplitude with a fixed elasticity and stress-relaxation timescale. Our study provides a universal route to tune the stress-relaxation of hydrogels without changing their components and elasticity, which may be valuable for systematic investigations of the stress-relaxation gradient in cell cultures and organoid constructions.
Text Classification Using Intuitionistic Fuzzy Set Measures—An Evaluation Study
Authors: Sidiropoulos George K. , Diamianos Nikolaos , Apostolidis Kyriakos D. , Papakostas George A.
Published: May 2022

A very important task of Natural Language Processing is text categorization (or text classification), which aims to automatically classify a document into categories. This kind of task includes numerous applications, such as sentiment analysis, language or intent detection, heavily used by social-/brand-monitoring tools, customer service, and the voice of customer, among others. Since the introduction of Fuzzy Set theory, its application has been tested in many fields, from bioinformatics to industrial and commercial use, as well as in cases with vague, incomplete, or imprecise data, highlighting its importance and usefulness in the fields. The most important aspect of the application of Fuzzy Set theory is the measures employed to calculate how similar or dissimilar two samples in a dataset are. In this study, we evaluate the performance of 43 similarity and 19 distance measures in the task of text document classification, using the widely used BBC News and BBC Sports benchmark datasets. Their performance is optimized through hyperparameter optimization techniques and evaluated via a leave-one-out cross-validation technique, presenting their performance using the accuracy, precision, recall, and F1-score metrics.
The Upshot of the SARS-CoV-2 Pandemic on Nursing Assistants: Evaluating Mental Health Indicators in Huelva
Authors: Gago-Valiente Francisco-Javier , Moreno-Sánchez Emilia , Vélez-Moreno Emilia , Merino-Godoy María-de-los-Ángeles , Sáez-Padilla Jesús , de Paula Rodríguez-Miranda Francisco , da Costa Emília Isabel Martins Teixeira , Saenz-de-la-Torre Luis-Carlos , Segura-Camacho Adrián , Mendoza-Sierra María-Isabel
Published: May 2022

Healthcare professionals who work in front-line situations are among those under the highest risk of presenting negative mental health indicators. We sought to assess the prevalence of low personal realization, emotional exhaustion, and depersonalization as well as probable non-psychotic psychiatric pathologies during the pandemic in nursing assistants in the city of Huelva (Spain), and to study the association between these mental health indicators and sociodemographic and professional variables. A cross-sectional descriptive investigation with a quantitative approach was used. A representative sample of these professionals, consisting of 29 men and 284 women, completed the GHQ-12 questionnaire, including sociodemographic data and the MBI-HSS questionnaire, collecting information on situations of contact with SARS-CoV-2. Data analysis was conducted, and correlations were established. We found that emotional exhaustion, depersonalization and probable non-psychotic, psychiatric pathologies were related to contact with SARS-CoV-2. Moreover, personal realization, depersonalization and emotional exhaustion were related to just gender. We conclude that nursing assistants from public hospitals in the city of Huelva who had contact with patients with SARS-CoV-2 in the workplace, showed poor mental health indicators than those who did not come into contact with infected individuals.