bluuebunny/crossref_metadata_2025 huggingface dataset:
Author Correction: Susceptible genes and disease mechanisms identified in frontotemporal dementia and frontotemporal dementia with Amyotrophic Lateral Sclerosis by DNA-methylation and GWAS
Authors: Taskesen E. , Mishra A. , van der Sluis S. , Ferrari R. ,   International FTD-Genomics Consortium, Hernandez D. G. , Nalls M. A. , Rohrer J. D. , Ramasamy A. , Kwok J. B. J. , Dobson-Stone C. , Schofield P. R. , Halliday G. M. , Hodges J. R. , Piguet O. , Bartley L. , Thompson E. , Haan E. , Hernández I. , Ruiz A. , Boada M. , Borroni B. , Padovani A. , Cruchaga C. , Cairns N. J. , Benussi L. , Binetti G. , Ghidoni R. , Forloni G. , Albani D. , Galimberti D. , Fenoglio C. , Serpente M. , Scarpini E. , Clarimón J. , Lleó A. , Blesa R. , Waldö M. Landqvist , Nilsson K. , Nilsson C. , Mackenzie I. R. A. , Hsiung G.-Y. R. , Mann D. M. A. , Grafman J. , Morris C. M. , Attems J. , Griffiths T. D. , McKeith I. G. , Thomas A. J. , Pietrini P. , Huey E. D. , Wassermann E. M. , Baborie A. , Jaros E. , Tierney M. C. , Pastor P. , Razquin C. , Ortega-Cubero S. , Alonso E. , Perneczky R. , Diehl-Schmid J. , Alexopoulos P. , Kurz A. , Rainero I. , Rubino E. , Pinessi L. , Rogaeva E. , George-Hyslop P. St , Rossi G. , Tagliavini F. , Giaccone G. , Rowe J. B. , Schlachetzki J. C. M. , Uphill J. , Collinge J. , Mead S. , Danek A. , Van Deerlin V. M. , Grossman M. , Trojanowski J. Q. , van der Zee J. , Van Broeckhoven C. , Cappa S. F. , Leber I. , Hannequin D. , Golfier V. , Vercelletto M. , Brice A. , Nacmias B. , Sorbi S. , Bagnoli S. , Piaceri I. , Nielsen J. E. , Hjermind L. E. , Riemenschneider M. , Mayhaus M. , Ibach B. , Gasparoni G. , Pichler S. , Gu W. , Rossor M. N. , Fox N. C. , Warren J. D. , Spillantini M. G. , Morris H. R. , Rizzu P. , Heutink P. , Snowden J. S. , Rollinson S. , Richardson A. , Gerhard A. , Bruni A. C. , Maletta R. , Frangipane F. , Cupidi C. , Bernardi L. , Anfossi M. , Gallo M. , Conidi M. E. , Smirne N. , Rademakers R. , Baker M. , Dickson D. W. , Graff-Radford N. R. , Petersen R. C. , Knopman D. , Josephs K. A. , Boeve B. F. , Parisi J. E. , Seeley W. W. , Miller B. L. , Karydas A. M. , Rosen H. , van Swieten J. C. , Dopper E. G. P. , Seelaar H. , Scheltens P. , Logroscino G. , Capozzo R. , Novelli V. , Puca A. A , Franceschi M. , Postiglione A. , Milan G. , Sorrentino P. , Kristiansen M. , Chiang H.-H. , Graff C. , Pasquier F. , Rollin A. , Deramecourt V. , Lebouvier T. , Kapogiannis D. , Ferrucci L. , Pickering-Brown S. , Singleton A. B. , Hardy J. , Momeni P. , Veldink J. H. , van Es M. A. , Smit A. B. , Posthuma D. , Pijnenburg Y.
Published: May 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Circadian and Homeostatic Modulation of Multi-Unit Activity in Midbrain Dopaminergic Structures
Authors: Fifel Karim , Meijer Johanna H. , Deboer Tom
Published: May 2018

Although the link between sleep disturbances and dopamine (DA)-related neurological and neuropsychiatric disorders is well established, the impact of sleep alterations on neuronal activity of midbrain DA-ergic structures is currently unknown. Here, using wildtype C57Bl mice, we investigated the circadian- and sleep-related modulation of electrical neuronal activity in midbrain ventral-tegmental-area (VTA) and substantia nigra (SN). We found no significant circadian modulation of activity in SN while VTA displayed a low amplitude but significant circadian modulation with increased firing rates during the active phase. Combining neural activity recordings with electroencephalogram (EEG) recordings revealed a strong vigilance state dependent modulation of neuronal activity with increased activity during wakefulness and rapid eye movement sleep relative to non-rapid eye movement sleep in both SN and VTA. Six-hours of sleep deprivation induced a significant depression of neuronal activity in both areas. Surprisingly, these alterations lasted for up to 48 hours and persisted even after the normalization of cortical EEG waves. Our results show that sleep and sleep disturbances significantly affect neuronal activity in midbrain DA structures. We propose that these changes in neuronal activity underlie the well-known relationship between sleep alterations and several disorders involving dysfunction of the DA circuitry such as addiction and depression.
Author Correction: SMARCA2-regulated host cell factors are required for MxA restriction of influenza A viruses
Authors: Dornfeld Dominik , Dudek Alexandra H. , Vausselin Thibaut , Günther Sira C. , Hultquist Judd F. , Giese Sebastian , Khokhlova-Cubberley Daria , Chew Yap C. , Pache Lars , Krogan Nevan J. , Garcia-Sastre Adolfo , Schwemmle Martin , Shaw Megan L.
Published: May 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Author Correction: Grain and starch granule morphology in superior and inferior kernels of maize in response to nitrogen
Authors: Zhao Fucheng , Jing Liquan , Wang Decheng , Bao Fei , Lu Weiping , Wang Guiyue
Published: May 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes
Authors: Niehaus Marcel , Soltwisch Jens
Published: May 2018

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for the analysis of large biomolecules in numerous applications. The technique utilizes nanosecond-long laser pulses at various spot sizes to eject and ionize large molecules embedded in a highly absorptive chemical matrix. Despite the methods name, ‘molecular desorption’ from the matrix crystal surface is not the sole mechanism discussed for material ejection in MALDI, but additional ablation of larger clusters has been reported. Here we present results on the influence of laser fluence and spot size on the mechanisms of the initial material ejection in MALDI and subsequent plume development. We used a laser-based postionization (MALDI-2) as well as a complementary photoacoustic method to monitor the material ejection step. The photoacoustic data reveal a quasi-thermal sublimation process up to a transition fluence. Above this threshold fluence additional ablation processes are observed. Complementary investigations on plume dynamics by MALDI-2 showed an ejection of predominantly fast particles for desorption conditions while ablation produces considerably slower ejecta. Additionally the presented results revealed a peculiar influence of the spot size on analyte fragmentation as well as plume development and allows for new insights into the unexplained spot size effect reported for MALDI.
Origin of magnetic properties in carbon implanted ZnO nanowires
Authors: Wang Y. F. , Shao Y. C. , Hsieh S. H. , Chang Y. K. , Yeh P. H. , Hsueh H. C. , Chiou J. W. , Wang H. T. , Ray S. C. , Tsai H. M. , Pao C. W. , Chen C. H. , Lin H. J. , Lee J. F. , Wu C. T. , Wu J. J. , Chang Y. M. , Asokan K. , Chae K. H. , Ohigashi T. , Takagi Y. , Yokoyama T. , Kosugi N. , Pong W. F.
Published: May 2018

Various synchrotron radiation-based spectroscopic and microscopic techniques are used to elucidate the room-temperature ferromagnetism of carbon-doped ZnO-nanowires (ZnO-C:NW) via a mild C+ ion implantation method. The photoluminescence and magnetic hysteresis loops reveal that the implantation of C reduces the number of intrinsic surface defects and increases the saturated magnetization of ZnO-NW. The interstitial implanted C ions constitute the majority of defects in ZnO-C:NW as confirmed by the X-ray absorption spectroscopic studies. The X-ray magnetic circular dichroism spectra of O and C K-edge respectively indicate there is a reduction in the number of unpaired/dangling O 2p bonds in the surface region of ZnO-C:NW and the C 2p-derived states of the implanted C ions strongly affect the net spin polarization in the surface and bulk regions of ZnO-C:NW. Furthermore, these findings corroborate well with the first-principles calculations of C-implanted ZnO in surface and bulk regions, which highlight the stability of implanted C for the suppression and enhancement of the ferromagnetism of the ZnO-C:NW in the surface region and bulk phase, respectively.
Electrical synapses between inhibitory neurons shape the responses of principal neurons to transient inputs in the thalamus: a modeling study
Authors: Pham Tuan , Haas Julie S.
Published: May 2018

As multimodal sensory information proceeds to the cortex, it is intercepted and processed by the nuclei of the thalamus. The main source of inhibition within thalamus is the reticular nucleus (TRN), which collects signals both from thalamocortical relay neurons and from thalamocortical feedback. Within the reticular nucleus, neurons are densely interconnected by connexin36-based gap junctions, known as electrical synapses. Electrical synapses have been shown to coordinate neuronal rhythms, including thalamocortical spindle rhythms, but their role in shaping or modulating transient activity is less understood. We constructed a four-cell model of thalamic relay and TRN neurons, and used it to investigate the impact of electrical synapses on closely timed inputs delivered to thalamic relay cells. We show that the electrical synapses of the TRN assist cortical discrimination of these inputs through effects of truncation, delay or inhibition of thalamic spike trains. We expect that these are principles whereby electrical synapses play similar roles in regulating the processing of transient activity in excitatory neurons across the brain.
Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots
Authors: Chen Ziyan , Zhu Dong , Wu Jisu , Cheng Zhiwei , Yan Xing , Deng Xiong , Yan Yueming
Published: May 2018

In this study, we aimed to identify differentially accumulated proteins (DAPs) involved in PEG mock osmotic stress, cadmium (Cd2+) stress, and their combined stress responses in Brachypodium distachyon seedling roots. The results showed that combined PEG and Cd2+ stresses had more significant effects on Brachypodium seedling root growth, physiological traits, and ultrastructures when compared with each individual stress. Totally, 106 DAPs were identified that are responsive to individual and combined stresses in roots. These DAPs were mainly involved in energy metabolism, detoxification and stress defense and protein metabolism. Principal component analysis revealed that DAPs from Cd2+ and combined stress treatments were grouped closer than those from osmotic stress treatment, indicating that Cd2+ and combined stresses had more severe influences on the root proteome than osmotic stress alone. Protein–protein interaction analyses highlighted a 14-3-3 centered sub-network that synergistically responded to osmotic and Cd2+ stresses and their combined stresses. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 14 key DAP genes revealed that most genes showed consistency between transcriptional and translational expression patterns. A putative pathway of proteome metabolic changes in Brachypodium seedling roots under different stresses was proposed, which revealed a complicated synergetic responsive network of plant roots to adverse environments.
Auxin decreases chromatin accessibility through the TIR1/AFBs auxin signaling pathway in proliferative cells
Authors: Hasegawa Junko , Sakamoto Takuya , Fujimoto Satoru , Yamashita Tomoe , Suzuki Takamasa , Matsunaga Sachihiro
Published: May 2018

Chromatin accessibility is closely associated with chromatin functions such as gene expression, DNA replication, and maintenance of DNA integrity. However, the relationship between chromatin accessibility and plant hormone signaling has remained elusive. Here, based on the correlation between chromatin accessibility and DNA damage, we used the sensitivity to DNA double strand breaks (DSBs) as an indicator of chromatin accessibility and demonstrated that auxin regulates chromatin accessibility through the TIR1/AFBs signaling pathway in proliferative cells. Treatment of proliferating plant cells with an inhibitor of the TIR1/AFBs auxin signaling pathway, PEO-IAA, caused chromatin loosening, indicating that auxin signaling functions to decrease chromatin accessibility. In addition, a transcriptome analysis revealed that severalhistone H4genes and a histone chaperone gene,FAS1, are positively regulated through the TIR1/AFBs signaling pathway, suggesting that auxin plays a role in promoting nucleosome assembly. Analysis of thefas1mutant ofArabidopsis thalianaconfirmed that FAS1 is required for the auxin-dependent decrease in chromatin accessibility. These results suggest that the positive regulation of chromatin-related genes mediated by the TIR1/AFBs auxin signaling pathway enhances nucleosome assembly, resulting in decreased chromatin accessibility in proliferative cells.
Nanojunction Effects on Water Flow in Carbon Nanotubes
Authors: Ebrahimi Fatemeh , Ramazani Farzaneh , Sahimi Muhammad
Published: May 2018

We report on the results of extensive molecular dynamics simulation of water imbibition in carbon nanotubes (CNTs), connected together by converging or diverging nanojunctions in various configurations. The goal of the study is to understand the effect of the nanojunctions on the interface motion, as well as the differences between what we study and water imbibition in microchannels. While the dynamics of water uptake in the entrance CNT is the same as that of imbibition in straight CNTs, with the main source of energy dissipation being the friction at the entrance, water uptake in the exit CNT is more complex due to significant energy loss in the nanojunctions. We derive an approximate but accurate expression for the pressure drop in the nanojunction. A remarkable difference between dynamic wetting of nano- and microjunctions is that, whereas water absorption time in the latter depends only on the ratios of the radii and of the lengths of the channels, the same is not true about the former, which is shown to be strongly dependent upon the size of each segment of the nanojunction. Interface pinning-depinning also occurs at the convex edges.
Melanopsin-mediated pupil function is impaired in Parkinson’s disease
Authors: Joyce Daniel S. , Feigl Beatrix , Kerr Graham , Roeder Luisa , Zele Andrew J.
Published: May 2018

Parkinson’s disease (PD) is characterised by non-motor symptoms including sleep and circadian disruption. Melanopsin-expressing intrinsically photosensitive Retinal Ganglion Cells (ipRGC) transmit light signals to brain areas controlling circadian rhythms and the pupil light reflex. To determine if non-motor symptoms observed in PD are linked to ipRGC dysfunction, we evaluated melanopsin and rod/cone contributions to the pupil response in medicated participants with PD (n = 17) and controls (n = 12). Autonomic tone was evaluated by measuring pupillary unrest in darkness. In the PD group, there is evidence for an attenuated post-illumination pupil response (PIPR) amplitude and reduced pupil constriction amplitude, and PIPR amplitudes did not correlate with measures of sleep quality, retinal nerve fibre layer thickness, disease severity, or medication dosage. Both groups exhibited similar pupillary unrest. We show that melanopsin- and the rod/cone-photoreceptor contributions to the pupil control pathway are impaired in people with early-stage PD who have no clinically observable ophthalmic abnormalities. Given that ipRGCs project to brain targets involved in arousal, sleep and circadian rhythms, ipRGC dysfunction may underpin some of the non-motor symptoms observed in PD.
Loss of live coral compromises predator-avoidance behaviour in coral reef damselfish
Authors: Boström-Einarsson Lisa , Bonin Mary C. , Munday Philip L. , Jones Geoffrey P.
Published: May 2018

Tropical reefs have experienced an unprecedented loss of live coral in the past few decades and the biodiversity of coral-dependent species is under threat. Many reef fish species decline in abundance as coral cover is lost, yet the mechanisms responsible for these losses are largely unknown. A commonly hypothesised cause of fish decline is the loss of shelter space between branches as dead corals become overgrown by algae. Here we tested this hypothesis by quantifying changes in predator-avoidance behaviour of a common damselfish, Pomacentrus moluccensis, before and after the death of their coral colony. Groups of P. moluccensis were placed on either healthy or degraded coral colonies, startled using a visual stimulus and their sheltering responses compared over a 7-week period. P. moluccensis stopped sheltering amongst the coral branches immediately following the death of the coral, despite the presence of ample shelter space. Instead, most individuals swam away from the dead coral, potentially increasing their exposure to predators. It appears that the presence of live coral rather than shelter per se is the necessary cue that elicits the appropriate behavioural response to potential predators. The disruption of this link poses an immediate threat to coral-associated fishes on degrading reefs.
DNAJC17 is localized in nuclear speckles and interacts with splicing machinery components
Authors: Pascarella A. , Ferrandino G. , Credendino S. C. , Moccia C. , D’Angelo F. , Miranda B. , D’Ambrosio C. , Bielli P. , Spadaro O. , Ceccarelli M. , Scaloni A. , Sette C. , De Felice M. , De Vita G. , Amendola E.
Published: May 2018

DNAJC17 is a heat shock protein (HSP40) family member, identified in mouse as susceptibility gene for congenital hypothyroidism. DNAJC17 knockout mouse embryos die prior to implantation. In humans, germline homozygous mutations in DNAJC17 have been found in syndromic retinal dystrophy patients, while heterozygous mutations represent candidate pathogenic events for myeloproliferative disorders. Despite widespread expression and involvement in human diseases, DNAJC17 function is still poorly understood. Herein, we have investigated its function through high-throughput transcriptomic and proteomic approaches. DNAJC17-depleted cells transcriptome highlighted genes involved in general functional categories, mainly related to gene expression. Conversely, DNAJC17 interactome can be classified in very specific functional networks, with the most enriched one including proteins involved in splicing. Furthermore, several splicing-related interactors, were independently validated by co-immunoprecipitation and in vivo co-localization. Accordingly, co-localization of DNAJC17 with SC35, a marker of nuclear speckles, further supported its interaction with spliceosomal components. Lastly, DNAJC17 up-regulation enhanced splicing efficiency of minigene reporter in live cells, while its knockdown induced perturbations of splicing efficiency at whole genome level, as demonstrated by specific analysis of RNAseq data. In conclusion, our study strongly suggests a role of DNAJC17 in splicing-related processes and provides support to its recognized essential function in early development.
Predicting transmission blocking potential of anti-malarial compounds in the Mosquito Feeding Assay using Plasmodium falciparum Male Gamete Inhibition Assay
Authors: Colmenarejo Gonzalo , Lozano Sonia , González-Cortés Carolina , Calvo David , Sanchez-Garcia Juliana , Matilla Jesús-Luís Presa , Leroy Didier , Rodrigues Janneth
Published: May 2018

Plasmodium falciparum Standard Membrane Feeding Assay (PfSMFA) is the current gold standard mosquito based confirmatory transmission blocking (TrB) assay for human malaria. However, owing to its complexity only selected gametocytocidal molecules are progressed into SMFA. Predictive tools for evaluation of TrB behavior of compounds in SMFA would be extremely beneficial, but lack of substantially large data sets from many mosquito feeds preempts the ability to perform correlations between outcomes from in vitro assays and SMFA. Here, a total of 44 different anti-malarial compounds were screened for inhibitory effect on male gamete formation in exflagellation inhibition assay (EIA) and the same drug-treated parasites were fed to mosquitoes in SMFA. Regression analysis was performed between outcomes of the two assays and regression models were applied to a randomly selected validation set of four compounds indicating no overfitting and good predictive power. In addition, the pIC50 for 11 different compounds obtained in the EIA was also correlated with pIC50’s in SMFA. Resulting regression models provided pIC50 predictions in SMFA with reasonably good accuracy thereby demonstrating the use of a simple in vitro assay to predict TrB of molecules in a complex mosquito based assay.
Experimental investigation of radiobiology in head and neck cancer cell lines as a function of HPV status, by MTT assay
Authors: Reid Paul , Wilson Puthenparampil , Li Yanrui , Marcu Loredana G. , Staudacher Alexander H. , Brown Michael P. , Bezak Eva
Published: May 2018

Head and neck cancers (HNCs) are aggressive epithelial tumours frequently treated using radiation. HNC biology shows distinctions dependent on the oncologic involvement of the human papilloma virus (HPV). Clinically, HPV positive HNCs respond better to radiotherapy but few in vitro data demonstrate radiobiological differences explaining differences in clinical outcomes. This pilot study examined radiobiological responses to irradiation and subsequent regeneration in two HNC cell lines (HPV positive and negative). A novel approach was taken to develop generational cultures of HNC cell lines, UM-SCC-1 (HPV negative) and UM-SCC-47 (HPV positive). MTT assays were used to determine surviving metabolic activity as a function of dose following 6 MV X-ray irradiation. Parallel cultures surviving 4 Gy irradiation (not analysed) were re-cultured and passaged to develop subsequent generations which were re-irradiated and analysed for generational change in radiation response. Second and 3rd generations of UM-SCC-1 showed decreasing metabolic activity with dose but little difference was evident in surviving fractions between these generations. Significantly lower metabolic activity in the 3rd generation at <6 Gy, compared to the 2nd generation, showed UM-SCC-47 becoming progressively more radiosensitive. HPV positive UM-SCC-47 showed generational progression in radiosensitisation not seen in the HPV negative UM-SCC-1.
RNA-Seq and UHPLC-Q-TOF/MS Based Lipidomics Study in Lysiphlebia japonica
Authors: Gao Xueke , Luo Junyu , Lü Limin , Zhang LiJuan , Zhang Shuai , Cui Jinjie
Published: May 2018

Lipids play an important role in energy storage, membrane structure stabilization and signaling. Parasitoids are excellent models to study lipidomics because a majority of them do not accumulate during their free-living life-stage. Studies on parasitoids have mostly focused on the changes in the lipids and gene transcripts in hosts and little attention has been devoted to lipidomics and transcriptomics changes in parasitoids. In this study, a relative quantitative analysis of lipids and their gene transcripts in 3-days-old Lysiphlebia japonica larva (3 days after spawning) and pupae were performed using liquid chromatography, mass spectrometry and RNA-seq. Thirty-three glycerolipids and 250 glycerophospholipids were identified in this study; all triglycerides and the vast majority of phospholipids accumulated in the pupal stage. This was accompanied by differentially regulated lipid uptake and remolding. Furthermore, our data showed that gene transcription was up-regulated in key nutrient metabolic pathways involved in lipid synthesis in 3-days-old larvae. Finally, our data suggests that larva and pupa of L. japonica may lack the ability for fatty acids synthesis. A comprehensive, quantitative, and expandable resource was provided for further studies of metabolic regulation and molecular mechanisms underlying parasitic response to hosts defense.
Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis
Authors: Hu Zihua , Jiang Kaiyu , Frank Mark Barton , Chen Yanmin , Jarvis James N.
Published: May 2018

Neutrophils in children with the polyarticular form of juvenile idiopathic arthritis (JIA) display abnormal transcriptional patterns linked to fundamental metabolic derangements. In this study, we sought to determine the effects of therapy on mRNA and miRNA expression networks in polyarticular JIA. Using exon and miRNA microarrays, we studied children with untreated active JIA (ADU, n = 35), children with active disease on therapy with methotrexate ± etanercept (ADT, n = 26), and children with inactive disease also on therapy (ID, n = 14). We compared the results to findings from healthy control children (HC, n = 35). We found substantial re-ordering of mRNA and miRNA expression networks after the initiation of therapy. Each disease state was associated with a distinct transcriptional profile, with the ADT state differing the most from HC, and ID more strongly resembling HC. Changes at the mRNA level were mirrored in changes in miRNA expression patterns. The analysis of the expression dynamics from differentially expressed genes across three disease states indicated that therapeutic response is a complex process. This process does not simply involve genes slowly correcting in a linear fashion over time. Computational modeling of miRNA and transcription factor (TF) co-regulatory networks demonstrated that combinational regulation of miRNA and TF might play an important role in dynamic transcriptome changes.
Development and evaluation of a novel system for inducing orthostatic challenge by tilt tests and lower body negative pressure
Authors: Dziuda Łukasz , Krej Mariusz , Śmietanowski Maciej , Sobotnicki Aleksander , Sobiech Mariusz , Kwaśny Piotr , Brzozowska Anna , Baran Paulina , Kowalczuk Krzysztof , Skibniewski Franciszek W.
Published: May 2018

Lower body negative pressure (LBNP) is a method derived from space medicine, which in recent years has been increasingly used by clinicians to assess the efficiency of the cardiovascular regulatory mechanisms. LBNP with combined tilt testing is considered as an effective form of training to prevent orthostatic intolerance. We have developed a prototype system comprising a tilt table and LBNP chamber, and tested it in the context of the feasibility of the device for assessing the pilots’ efficiency. The table allows for controlled tilting in the range from −45 to +80° at the maximum change rate of 45°/s. The LBNP value can smoothly be adjusted down to −100 mmHg at up to 20 mmHg/s. 17 subjects took part in the pilot study. A 24-minute scenario included −100 mmHg supine LBNP, head up tilt (HUT) and −60 mmHg LBNP associated with HUT, separated by resting phases. The most noticeable changes were observed in stroke volume (SV). During supine LBNP, HUT and the combined stimulus, a decrease of the SV value by 20%, 40% and below 50%, respectively, were detected. The proposed system can map any pre-programed tilt and LBNP profiles, and the pilot study confirmed the efficiency of performing experimental procedures.
Nanofocusing of structured light for quadrupolar light-matter interactions
Authors: Sakai Kyosuke , Yamamoto Takeaki , Sasaki Keiji
Published: May 2018

The spatial structure of an electromagnetic field can determine the characteristics of light-matter interactions. A strong gradient of light in the near field can excite dipole-forbidden atomic transitions, e.g., electric quadrupole transitions, which are rarely observed under plane-wave far-field illumination. Structured light with a higher-order orbital angular momentum state may also modulate the selection rules in which an atom can absorb two quanta of angular momentum: one from the spin and another from the spatial structure of the beam. Here, we numerically demonstrate a strong focusing of structured light with a higher-order orbital angular momentum state in the near field. A quadrupole field was confined within a gap region of several tens of nanometres in a plasmonic tetramer structure. A plasmonic crystal surrounding the tetramer structure provides a robust antenna effect, where the incident structured light can be strongly coupled to the quadrupole field in the gap region with a larger alignment tolerance. The proposed system is expected to provide a platform for light-matter interactions with strong multipolar effects.
Novel pathway of 3-hydroxyanthranilic acid formation in limazepine biosynthesis reveals evolutionary relation between phenazines and pyrrolobenzodiazepines
Authors: Pavlikova Magdalena , Kamenik Zdenek , Janata Jiri , Kadlcik Stanislav , Kuzma Marek , Najmanova Lucie
Published: May 2018

Natural pyrrolobenzodiazepines (PBDs) form a large and structurally diverse group of antitumour microbial metabolites produced through complex pathways, which are encoded within biosynthetic gene clusters. We sequenced the gene cluster of limazepines and proposed their biosynthetic pathway based on comparison with five available gene clusters for the biosynthesis of other PBDs. Furthermore, we tested two recombinant proteins from limazepine biosynthesis, Lim5 and Lim6, with the expected substrates in vitro. The reactions monitored by LC-MS revealed that limazepine biosynthesis involves a new way of 3-hydroxyanthranilic acid formation, which we refer to as the chorismate/DHHA pathway and which represents an alternative to the kynurenine pathway employed for the formation of the same precursor in the biosynthesis of other PBDs. The chorismate/DHHA pathway is presumably also involved in the biosynthesis of PBD tilivalline, several natural products unrelated to PBDs, and its part is shared also with phenazine biosynthesis. The similarities between limazepine and phenazine biosynthesis indicate tight evolutionary links between these groups of compounds.
Comparative genomic analysis of Acinetobacter spp. plasmids originating from clinical settings and environmental habitats
Authors: Salto Ileana P. , Torres Tejerizo Gonzalo , Wibberg Daniel , Pühler Alfred , Schlüter Andreas , Pistorio Mariano
Published: May 2018

Bacteria belonging to the genus Acinetobacter have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among Acinetobacter strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental Acinetobacter spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all Acinetobacter Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQAci, composed exclusively of Acinetobacter relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.
Terahertz-driven polymerization of resists in nanoantennas
Authors: Park Woongkyu , Lee Youjin , Kang Taehee , Jeong Jeeyoon , Kim Dai-Sik
Published: May 2018

Plasmon-mediated polymerization has been intensively studied for various applications including nanolithography, near-field mapping, and selective functionalization. However, these studies have been limited from the near-infrared to the ultraviolet regime. Here, we report a resist polymerization using intense terahertz pulses and various nanoantennas. The resist is polymerized near the nanoantennas, where giant field enhancement occurs. We experimentally show that the physical origin of the cross-linking is a terahertz electron emission from the nanoantenna, rather than multiphoton absorption. Our work extends nano-photochemistry into the terahertz frequencies.
Correction: Corrigendum: The Phosphorylation Profile of Myosin Binding Protein-C Slow is Dynamically Regulated in Slow-Twitch Muscles in Health and Disease
Authors: Ackermann Maegen A. , Kerr Jaclyn P. , King Brendan , Ward Christopher W. , Kontrogianni-Konstantopoulos Aikaterini
Published: May 2018

Scientific Reports 5: Article number: 12637; published online: 19 August 2015; updated: 11 May 2018 This Article contains errors in Figure 2, where the blots for (−) GST-sMyBP-C NH2 aa1-285, (+) GST-fMyBP-C NH2 aa1-249, (−) GST-fMyBP-C NH2 aa1-249, (+) GST-protein and (−) GST-protein (the right halfof the panel) were duplicated between all panels.
Phosphonium carbosilane dendrimers – interaction with a simple biological membrane model
Authors: Wrobel Dominika , Kubikova Radka , Müllerová Monika , Strašák Tomas , Růžička Květoslav , Fulem Michal , Maly Jan
Published: May 2018

Factors such as shielding of charge on dendrimers by bulky substituents and/or hydrophobicity of substituents are important for final ability of dendrimers to interact with and to penetrate deep into the lipid bilayer.
In situ fabrication of hollow ZnO@NC polyhedra from ZIF-8 for the determination of trace Cd(ii)
Authors: Zhang Xueping , Wang Qingqing , Li Jing , Huang Liang , Yu Dengbin , Dong Shaojun
Published: May 2018

H-ZnO@NC polyhedra prepared from ZIF-8 exhibit remarkable analytical performances for the electrochemical detection of Cd(ii).
Iptycene substitution enhances the electrochemical activity and stability of polyanilines
Authors: Tan Wei Shyang , Lee Ting-Yin , Hsu Ying-Feng , Huang Shing-Jong , Yang Jye-Shane
Published: May 2018

The clipping effect of pentiptycene cavities provides a significant improvement in pseudocapacitance and charge–discharge stability for pentiptycene-incorporated polyanilines.
Imperfect mixing as a dominant factor leading to stochastic behavior: a new system exhibiting crazy clock behavior
Authors: Valkai László , Horváth Attila K.
Published: May 2018

The stochastic feature of the arsenous acid–periodate reaction is shown both experimentally and numerically to be originated from imperfect mixing and initial inhomogeneity.
A freestanding SiO2 ultrathin membrane with NiCu nanoparticles embedded on its double surfaces for catalyzing nitro-amination
Authors: Gu Chen , Wu Dandan , Wen Ming , Wu Qingsheng
Published: May 2018

A 2D ultrathin-structured NiCu–SiO2 nanocomposite formed by assembling NiCu nanoparticles on the double surfaces of a freestanding SiO2 membrane achieves excellent catalytic performance for nitro-amination reactions.
Conducting single-molecule magnet materials
Authors: Cosquer Goulven , Shen Yongbing , Almeida Manuel , Yamashita Masahiro
Published: May 2018

Multifunctional molecular materials exhibiting electrical conductivity and single-molecule magnet (SMM) behaviour are particularly attractive for electronic devices and related applications owing to the interaction between electronic conduction and magnetization of unimolecular units.
Amphiphilic complexes of Ho(iii), Dy(iii), Tb(iii) and Eu(iii) for optical and high field magnetic resonance imaging
Authors: Harris Michael , Henoumont Céline , Peeters Wannes , Toyouchi Shuichi , Vander Elst Luce , Parac-Vogt Tatjana N.
Published: May 2018

Amphiphilic lanthanide(iii) complexes self-assemble into monodisperse micelles with favourable properties for optical and high field magnetic resonance imaging.
Three-dimensionally interconnected Si frameworks derived from natural halloysite clay: a high-capacity anode material for lithium-ion batteries
Authors: Wan Hao , Xiong Hao , Liu Xiaohe , Chen Gen , Zhang Ning , Wang Haidong , Ma Renzhi , Qiu Guanzhou
Published: May 2018

Three-dimensionally interconnected (3D-interconnected) Si frameworks with a branch diameter of ∼15 nm were successfully prepared using a molten-salt reduction process.
An octahedral tetrachlorido Fe(ii) complex with aminopyrazinium ligands from a serendipitous redox synthesis exhibiting magnetic exchange through non-covalent 3-D architectures
Authors: Rusbridge Elizabeth K. , Peng Yan , Powell Annie K. , Robinson David , Fitzpatrick Anthony J.
Published: May 2018

An air stable, neutral Fe(ii) complex with four equatorial chlorido ligands has been stabilised through a serendipitous redox process and in situ ligand protonation.
Hybrid organic–inorganic connectivity of NdIII(pyrazine-N,N′-dioxide)[CoIII(CN)6]3− coordination chains for creating near-infrared emissive Nd(iii) showing field-induced slow magnetic relaxation
Authors: Chorazy Szymon , Charytanowicz Tomasz , Wang Junhao , Ohkoshi Shin-ichi , Sieklucka Barbara
Published: May 2018

Organic pzdo and inorganic hexacyanidocobaltate(iii) support the formation of Nd(iii) complexes showing NIR fluorescence and magnetic anisotropy.
Solvent-induced ion separation of a beryllium scorpionate complex
Authors: Naglav Dominik , Tobey Briac , Dzialkowski Kevin , Bläser Dieter , Wölper Christoph , Jansen Georg , Schulz Stephan
Published: May 2018

TpBeI undergoes a spontaneous ion separation upon treatment with THF, yielding [TpBe(thf)]I, which represents a rare example of a cationic beryllium complex.
Electrochromic capacitive windows based on all conjugated polymers for a dual function smart window
Authors: Kim Younghoon , Han Minsu , Kim Jinbo , Kim Eunkyoung
Published: May 2018

A highly transparent electrochromic capacitive (ECC) window was explored by combining a high contrast electrochromic polymer (ECP) and a transparent capacitive polymer.
Degradation mechanisms of cefotaxime using biochar supported Co/Fe bimetallic nanoparticles
Authors: Wu Hongwei , Feng Qiyan , Lu Ping , Chen Meng , Yang Hong
Published: May 2018

The synergetic reaction of adsorption and reduction caused cefotaxime efficient degradation by Co/Fe/MB and the degradation pathway was also analyzed.
Dimeric palladium 1,2,3-triazol-5-ylidene complexes – synthesis, structure, reactivity and catalytic properties in Suzuki coupling
Authors: Lorkowski Jan , Żak Patrycja , Kubicki Maciej , Pietraszuk Cezary , Jędrzkiewicz Dawid , Ejfler Jolanta
Published: May 2018

Palladium 1,2,3-triazol-5-ylidene dimers transform to bis(carbene) species and in Suzuki coupling conditions lose carbeneviareductive elimination of a 5-aryltriazolium salt.
A pyrazoline-based fluorescent chemosensor for Al3+ ion detection and live cell imaging
Authors: Rangasamy Manjunath , Palaninathan Kannan
Published: May 2018

The pyrene appended pyrazoline compounds were designed and synthesized for selective detection of Al3+ ion.
One-pot reductive amination of aldehydes with nitroarenes using formic acid as the hydrogen donor and mesoporous graphitic carbon nitride supported AgPd alloy nanoparticles as the heterogeneous catalyst
Authors: Ergen Seda , Nişancı Bilal , Metin Önder
Published: May 2018

A facile one-pot protocol has been developed for the synthesis of secondary amines via a tandem reductive amination of aldehydes with nitroaromatics.
Four alkali metal molybdates with two types of Mo–O chains, ABMo3O10 (A = Li, B = Rb; A = Li, Na, K, B = Cs): synthesis, structure comparison and optical properties
Authors: Han Shujuan , Zhang Bingbing , Tong Tinghao , Yang Zhihua , Pan Shilie
Published: May 2018

The effects of cation size on the framework structures of four stoichiometrically equivalent alkali metal molybdates were discussed in detail.
A theranostic nanoplatform: magneto-gold@fluorescence polymer nanoparticles for tumor targeting T1&T2-MRI/CT/NIR fluorescence imaging and induction of genuine autophagy mediated chemotherapy
Authors: Wang Guannan , Qian Kun , Mei Xifan
Published: May 2018

Novel contrast agent were designed for in vivo T1&T2-MRI/CT/NIR fluorescence imaging and induction of genuine autophagy-mediated chemosensitization for cancer therapy.
Three-dimensional sp2 carbon networks prepared by ultrahigh temperature treatment for ultrafast lithium–sulfur batteries
Authors: Ruan Chunping , Yang Zhi , Nie Huagui , Zhou Xuemei , Guo Zeqing , Wang Lu , Ding Xingwei , Chen Xi'an , Huang Shaoming
Published: May 2018

3D carbon nanotubes/graphene prepared by ultrahigh temperature treatment with S (2850CNTs–Gra–S) for application in Li–S batteries. The 2850CNTs–Gra–S cathode delivers a considerable specific capacity after 1500 cycles at 15C.
Nitric oxide release from a cucurbituril encapsulated NO-donor
Authors: Acuña A. , Basílio N. , Parajó M. , Mejuto J. C. , Pérez-Juste J. , Taladriz-Blanco P. , Garcia-Rio L.
Published: May 2018

The denitrosation of a S-nitrosothiol derivative, nitrosomercaptopyridine (SNO+), can be inhibited by incorporation into the cucurbit[7]uril cavity. Owing to the reversible character of host : guest complexation, SNO+can be expelled from the host cavity through the application of a chemical stimulus allowing controlled nitric oxide release.
pH and reduction-activated polymeric prodrug nanoparticles based on a 6-thioguanine-dialdehyde sodium alginate conjugate for enhanced intracellular drug release in leukemia
Authors: Wan Yanming , Bu Ying , Liu Jiaming , Yang Jia , Cai Weiquan , Yin Yihua , Xu Wenjin , Xu Peihu , Zhang Jingli , He Meng
Published: May 2018

Synthesis schematics of DSA and 6-TG-DSA as well as processes of PPN self-assembly and its pH/GSH dual stimuli-response release of the conjugated 6-TG.
Facile synthesis of ultrathin and perpendicular NiMn2O4 nanosheets on reduced graphene oxide as advanced electrodes for supercapacitors
Authors: Li Long , Hu Hongli , Ding Shujiang
Published: May 2018

A NiMn2O4 NSs@rGO nanocomposite was successfully fabricated through a facile co-precipitation and thermal treatment process, which exhibits enhanced energy storage performance.
Understanding the influence of carboxylate substitution on the property of high-performance donor polymers in non-fullerene organic solar cells
Authors: Yang Guofang , Liu Jing , Ma Lik-Kuen , Chen Shangshang , Lai Joshua Yuk Lin , Ma Wei , Yan He
Published: May 2018

Carboxylate substitution can enhance crystallinity and introduce purer domains; alkyl chains provide a temperature-dependent aggregation property.
Efficient syntheses of alpha- and beta-C-nucleosides and the origin of anomeric selectivity
Authors: Liu Tongchao , Zhu Zhengdan , Ren Huanming , Chen Yabin , Chen Guohua , Cheng Maosheng , Zhao Dongmei , Shen Jingkang , Zhu Weiliang , Xiong Bing , Chen Yue-Lei
Published: May 2018

Valuable C-nucleosides are efficiently prepared with excellent anomeric selectivity depending on protecting groups on sugar moiety.
Comment on “Metal–organic green dye: chemical and physical insight into a modified Zn-benzoporphyrin for dye-sensitized solar cells” by G. Zanotti, N. Angelini, G. Mattioli, A. M. Paoletti, G. Pennesi, G. Rossi, D. Caschera, L. de Marcoc and G. Giglide, RSC Adv., 2016, 6, 5123
Authors: Steer Ronald P.
Published: May 2018

The emission spectroscopy and photophysics reported in the title paper are shown to be untenable in light of previously reported experimental results and widely accepted theories of electronic excited state relaxation.
Design, synthesis, and biological evaluation of AV6 derivatives as novel dual reactivators of latent HIV-1
Authors: Ao Mingtao , Pan Zhenrui , Qian Yuqing , Tang Bowen , Feng Zeming , Fang Hua , Wu Zhen , Chen Jingwei , Xue Yuhua , Fang Meijuan
Published: May 2018

As dual-acting HIV LRAs, compounds 12c and 12d could activate latent HIV-1 via the NFAT-required mechanism and as histone deacetylase (HDAC) inhibitors.
Fast quantification of fluoroquinolones in environmental water samples using molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry
Authors: Kou Wei , Zhang Hua , Bibi Aisha , Ke Mufang , Han Jing , Xiong Jianliang , Su Rui , Liang Dapeng
Published: May 2018

A simple, fast and high-sensitivity method for quantification of fluoroquinolones in environmental water samples using MIPs-iEESI-MS.
Synthesis, characterization, surface properties and energy device characterstics of 2D borocarbonitrides, (BN)xC1−x, covalently cross-linked with sheets of other 2D materials
Authors: Singh Navin Kumar , Pramoda K. , Gopalakrishnan K. , Rao C. N. R.
Published: May 2018

Covalent cross-linking of 2D structures such as graphene, MoS2 and C3N4 using coupling reactions affords the generation of novel materials with new or improved properties.
Photoluminescence mechanism and applications of Zn-doped carbon dots
Authors: Xu Quan , Cai Wei , Zhang Miaoran , Su Rigu , Ye Yingchun , Li Yeqing , Zhang Lipeng , Guo Yongjian , Yu Zhiqiang , Li Siyu , Lin Xun , Chen Yusheng , Luo Yan , Street Jason , Xu Meng
Published: May 2018

Zinc ions, acting as a surface passivating agent, prevented the aggregation of graphene π–π stacking and increased the quantum yield of Zn-carbon dots.
The effect of glycidyl azide polymer on the stability and explosive properties of different interesting nitramines
Authors: Hussein Ahmed K. , Elbeih Ahmed , Zeman Svatopluk
Published: May 2018

The preparation of glycidyl azide polymer (GAP) and its influence on the stability and explosive properties of polymer bonded explosives (PBXs) based on several cyclic nitramines (CL-20, RDX, HMX and BCHMX) are reported and discussed.
Europium ion post-functionalized zirconium metal–organic frameworks as luminescent probes for effectively sensing hydrazine hydrate
Authors: Yang Yunhui , Liu Xiaofei , Yan Dan , Deng Ping , Guo Zhiyong , Zhan Hongbing
Published: May 2018

A luminescent metal–organic framework have been realized for efficiently sensing hydrazine hydrate.
Rhodium-catalyzed asymmetric hydroamination and hydroindolation of keto-vinylidenecyclopropanes
Authors: Yang Song , Li Quan-Zhe , Xu Chen , Xu Qin , Shi Min
Published: May 2018

A novel rhodium-catalyzed asymmetric hydroamination and hydroindolation of keto-vinylidenecyclopropanes has been developed, affording the hydroamination and hydroindolation products in good to excellent yields with outstanding ee values through a new TMM–Rh model complex.
Tuning the mechanical properties of alginate–peptide hydrogels
Authors: Ochbaum Guy , Davidovich-Pinhas Maya , Bitton Ronit
Published: May 2018

Covalent bonding peptides to alginates is routinely used to tailor alginate's biofunctionality. Here, we investigate the possibility of tuning the mechanical properties of alginate–peptide gels by altering the sequence of the covalently bound peptide.
Hydrodynamic simulations of charge-regulation effects in colloidal suspensions
Authors: Takae Kyohei , Tanaka Hajime
Published: May 2018

Self-organization of charged soft matter is of crucial importance in biology.
Crossover from semi-dilute to densely packed thin polymer films at the air–water interface and structure formation at thin film breakup
Authors: Appel Christian , Kraska Martin , Rüttiger Christian , Gallei Markus , Stühn Bernd
Published: May 2018

First evidence for thin film breakup of a polymer film observed byin situSurface X-ray scattering at the air–water interface.
Diethyl(thiophen-2-ylmethyl)phosphonate: a novel multifunctional electrolyte additive for high voltage batteries
Authors: Zhu Yunmin , Luo Xueyi , Zhi Huozhen , Liao Youhao , Xing Lidan , Xu Mengqing , Liu Xiang , Xu Kang , Li Weishan
Published: May 2018

DTYP exhibits multiple functionalities that dramatically improve the performance of a high voltage Li-ion cell using LiNi0.5Mn1.5O4 at elevated temperature.
Flexible carbon dots composite paper for electricity generation from water vapor absorption
Authors: Li Qijun , Zhou Ming , Yang Qingfeng , Yang Mingyang , Wu Qian , Zhang Zhixun , Yu Jianwen
Published: May 2018

A scalable fabrication method for flexible moisture-electric energy transformation devices by dispersing or printing carbon dots on paper is reported.