bluuebunny/crossref_metadata_2025 huggingface dataset:
The potential use of non-fungible tokens (NFTs) in healthcare and medical research
Authors: Yaghy Antonio , Alberto Nicole Rose I. , Alberto Isabelle Rose I. , Bermea Rene S. , Ristovska Ljubica , Yaghy Maria , Hoyek Sandra , Patel Nimesh A. , Celi Leo Anthony
Published: July 2023

Non-fungible tokens (NFTs) are cryptographic assets recorded on the blockchain that can certify authenticity and ownership, and they can be used to monetize health data, optimize the process of receiving a hematopoietic stem cell transplant, and improve the distribution of solid organs for transplantation. Blockchain technology, including NFTs, provides equitable access to wealth, increases transparency, eliminates personal or institutional biases of intermediaries, reduces inefficiencies, and ensures accountability. Blockchain architecture is ideal for ensuring security and privacy while granting individuals jurisdiction over their own information, making it a unique solution to the current limitations of existing health information systems. NFTs can be used to give patients the option to monetize their health data and provide valuable data to researchers. Wearable technology companies can also give their customers the option to monetize their data while providing data necessary to improve their products. Additionally, the process of receiving a hematopoietic stem cell transplant and the distribution of solid organs for transplantation could benefit from the integration of NFTs into the allocation process. However, there are limitations to the technology, including high energy consumption and the need for regulatory guidance. Further research is necessary to fully understand the potential of NFTs in healthcare and how it can be integrated with existing health information technology. Overall, NFTs have the potential to revolutionize the healthcare sector, providing benefits such as improved access to health information and increased efficiency in the distribution of organs for transplantation.
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva
Authors: Shatilovich Anastasia , Gade Vamshidhar R. , Pippel Martin , Hoffmeyer Tarja T. , Tchesunov Alexei V. , Stevens Lewis , Winkler Sylke , Hughes Graham M. , Traikov Sofia , Hiller Michael , Rivkina Elizaveta , Schiffer Philipp H. , Myers Eugene W. , Kurzchalia Teymuras V.
Published: July 2023

Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus. Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis. Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P. kolymaensis and in C. elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C. elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales.
Health economic evaluation of strategies to eliminate gambiense human African trypanosomiasis in the Mandoul disease focus of Chad
Authors: Antillon Marina , Huang Ching-I , Sutherland Samuel A. , Crump Ronald E. , Bessell Paul R. , Shaw Alexandra P. M. , Tirados Iñaki , Picado Albert , Biéler Sylvain , Brown Paul E. , Solano Philippe , Mbainda Severin , Darnas Justin , Wang-Steverding Xia , Crowley Emily H. , Peka Mallaye , Tediosi Fabrizio , Rock Kat S.
Published: July 2023

Human African trypanosomiasis, caused by thegambiensesubspecies ofTrypanosoma brucei(gHAT), is a deadly parasitic disease transmitted by tsetse. Partners worldwide have stepped up efforts to eliminate the disease, and the Chadian government has focused on the previously high-prevalence setting of Mandoul. In this study, we evaluate the economic efficiency of the intensified strategy that was put in place in 2014 aimed at interrupting the transmission of gHAT, and we make recommendations on the best way forward based on both epidemiological projections and cost-effectiveness. In our analysis, we use a dynamic transmission model fit to epidemiological data from Mandoul to evaluate the cost-effectiveness of combinations of active screening, improved passive screening (defined as an expansion of the number of health posts capable of screening for gHAT), and vector control activities (the deployment of Tiny Targets to control the tsetse vector). For cost-effectiveness analyses, our primary outcome is disease burden, denominated in disability-adjusted life-years (DALYs), and costs, denominated in 2020 US$. Although active and passive screening have enabled more rapid diagnosis and accessible treatment in Mandoul, the addition of vector control provided good value-for-money (at less than $750/DALY averted) which substantially increased the probability of reaching the 2030 elimination target for gHAT as set by the World Health Organization. Our transmission modelling and economic evaluation suggest that the gains that have been made could be maintained by passive screening. Our analysis speaks to comparative efficiency, and it does not take into account all possible considerations; for instance, any cessation of ongoing active screening should first consider that substantial surveillance activities will be critical to verify the elimination of transmission and to protect against the possible importation of infection from neighbouring endemic foci.
Models of care and the role of clinical pharmacists in UK primary care for older adults: A scoping review protocol
Authors: Davies Nathan , Kolodin Vladimir , Woodward Abi , Bhanu Cini , Jani Yogini , Manthorpe Jill , Orlu Mine , Samsi Kritika , Burnand Alice , Vickerstaff Victoria , West Emily , Wilcock Jane , Rait Greta
Published: July 2023

Introduction

There has been global investment of new ways of working to support workforce pressures, including investment in clinical pharmacists working in primary care by the NHS in the England. Clinical pharmacists are well suited to support older adults who have multiple long-term conditions and are on multiple medications. It is important to establish an evidence base for the role of clinical pharmacists in supporting older adults in primary care, to inform strategic and research priorities. The aim of this scoping review is to identify, map and describe existing research and policy/guidance on the role of clinical pharmacists in primary care supporting older adults, and the models of care they provide.

Methods and analysis

A scoping review guided by the Joanne Briggs Institute methodology for scoping reviews, using a three-step strategy. We will search Medline, CINAHL, Scopus, EMBASE, Web of Science, PSYCHInfo, and Cochrane for English language articles, from 2015 –present day. Grey literature will be searched using Grey Matters guidelines, the Index of Grey Literature and Alternative Sources and Resources, and Google keyword searching. References of all included sources will be hand searched to identify further resources. Using the Population, Concept and Context framework for inclusion and exclusion criteria, articles will be independently screened by two reviewers. The inclusion and exclusion criteria will be refined after we become familiar with the search results, following the iterative nature of a scoping review. Data will be extracted using a data extraction tool using Microsoft Excel and presented using a narrative synthesis approach.

Ethics and dissemination

Ethical approval is not required for this review. Review findings will be disseminated in academic conferences and used to inform subsequent qualitative research. Findings will be published and shared with relevant local and national organisations.
Self-reported and measured anthropometric variables in association with cardiometabolic markers: A Danish cohort study
Authors: Zhang Jie , Olsen Anja , Halkjær Jytte , Petersen Kristina E. , Tjønneland Anne , Overvad Kim , Dahm Christina C.
Published: July 2023

General obesity is a recognized risk factor for various metabolically related diseases, including hypertension, dyslipidemia, and pre-diabetes. In epidemiological studies, anthropometric variables such as height and weight are often self-reported. However, misreporting of self-reported data may bias estimates of associations between anthropometry and health outcomes. Further, few validation studies have compared self-reported and measured waist circumference (WC). This study aimed to quantify the agreement between self-reported and measured height, weight, body mass index (BMI), WC, and waist-to-height ratio (WHtR), and to investigate associations of these anthropometric measures with cardiometabolic biomarkers. A total of 39,514 participants aged above 18 years were included into the Diet, Cancer, and Health-Next Generation Cohort in 2015–19. Self-reported and measured anthropometric variables, blood pressure, and cardiometabolic biomarkers (HbA1c, lipid profiles, C-reactive protein and creatinine) were collected by standard procedures. Pearson correlations (r) and Lin’s concordance correlations were applied to evaluate misreporting. Misreporting by age, sex and smoking status was investigated in linear regression models. Multivariable regression models and Receiver Operating Characteristic analyses assessed associations of self-reported and measured anthropometry with cardiometabolic biomarkers. Self-reported height was overreported by 1.07 cm, and weight was underreported by 0.32 kg on average. Self-reported BMI and WC were 0.42 kg/m2 and 0.2 cm lower than measured, respectively. Self-reported and measured height, weight, BMI, WC and WtHR were strongly correlated (r = 0.98, 0.99, 0.98, 0.88, 0.86, respectively). Age, sex, smoking, and BMI contributed to misreporting of all anthropometric measures. Associations between self-reported or measured anthropometric measures and cardiometabolic biomarkers were similar in direction and strength. Concordance between self-reported and measured anthropometric measures, including WC, was very high. Self-reported anthropometric measures were reliable when estimating associations with cardiometabolic biomarkers.
Harmonization of ICF Body Structures and ICD-11 Anatomic Detail: One foundation for multiple classifications
Authors: Della Mea Vincenzo , Almborg Ann-Helene , Martinuzzi Michela , Tu Samson W. , Martinuzzi Andrea
Published: July 2023

The Family of International Classifications of the World Health Organization (WHO-FIC) currently includes three reference classifications, namely International Classification of Diseases (ICD), International Classification of Functioning, Disability, and Health (ICF), and International Classification of Health Interventions (ICHI). Recently, the three classifications have been incorporated into a single WHO-FIC Foundation that serves as the repository of all concepts in the classifications. Each classification serves a specific classification need. However, they share some common concepts that are present, in different forms, in two or all of them. For the WHO-FIC Foundation to be a logically consistent repository without duplicates, these common concepts must be reconciled. One important set of shared concepts is the representation of human anatomy entities, which are not always modeled in the same way and with the same level of detail. To understand the relationships among the three anatomical representations, an effort is needed to compare them, identifying common areas, gaps, and compatible and incompatible modeling. The work presented here contributes to this effort, focusing on the anatomy representations in ICF and ICD-11. For this aim, three experts were asked to identify, for each entity in the ICF Body Structures, one or more entities in the ICD-11 Anatomic Detail that could be considered identical, broader or narrower. To do this, they used a specifically developed web application, which also automatically identified the most obvious equivalences. A total of 631 maps were independently identified by the three mappers for 218 ICF Body Structures, with an interobserver agreement of 93.5%. Together with 113 maps identified by the software, they were then consolidated into 434 relations. The results highlight some differences between the two classifications: in general, ICF is less detailed than ICD-11; ICF favors lumping of structures; in very few cases, the two classifications follow different anatomic models. For these issues, solutions have to be found that are compliant with the WHO approach to classification modeling and maintenance.
A highly accurate method for forecasting the compressor geometric variable system based on the data-driven method
Authors: Xia Cunjiang , Zhan Yuyou , Tan Yan , Gou Yi , Wu Wenqing
Published: July 2023

To make the puzzle of aero-engines complete, understanding the law of the compressor geometric variable system is a vital part. Modeling all aspects of aero-engines quickly has been a continuous area of research since the advent of artificial intelligence (AI). However, diagnosing or predicting faults is an ancient adage, and it is vital to explore key system forecast research, particularly since traditional forecasting techniques do not account for future information of non-target parameters. In this article, based on the feasibility of forecasting the compressor geometric variable system, an enhanced ConvNeXt model utilizing the Sliding Window Algorithm mechanism is proposed. And this method takes into account the future information of non-target parameters. With the novel strategy, the issue of the forecast’s error increasing with forecast length is alleviated. As a result, in a particular condition, the error we obtained only accounts for 20.07% of that of the standard forecast approach. Additionally, it is verified that this method can be applied to various aero-engines. Finally, experiments on several aero-engine states involving the transition state and the steady state are conducted to strengthen the plausibility and credibility of our theories. It should be noted that the foundation of each experiment is data from actual flights.
Evaluation of the coordinated development between Chinese cultural industry and scientific & technological innovation
Authors: Yu Zhenni , Yu Jian
Published: July 2023

Based on the coupling and interaction relationship between China’s cultural industry (CI) and scientific & technological innovation (STI), this study constructed an index system for their coordinated development. The weight of each indicator was determined by using the entropy value method (EVM), and the coupling coordination degree (CCD) model was used to calculate CCD and coordination degree of China’s CI and STI from 2012 to 2020. On this basis, the key factors in the coupling effect were analyzed using grey correlation degree (GCD). The results demonstrate that: (1) there is a high-level coupling relationship between China’s CI and technological innovation; (2) the level of coupling coordination between the two is generally on the rise, experiencing a development process from serious maladjustment to high coordination; (3) Industry resources, policy support and the cost of cultural undertakings are the endogenous factors restricting the development of CI, and the environment and output of STI are the key factors restricting the coupling and coordinated development of Chinese CI and STI.
The selection and validation of reference genes for quantitative real-time PCR studies in near-isogenic susceptible and resistant tomato lines, infected with the geminivirus tomato curly stunt virus
Authors: Bokhale Mamokete , Mwaba Imanu , Allie Farhahna
Published: July 2023

Quantitative real-time PCR (qPCR) is a sensitive and commonly used technique for gene expression profiling and provides insight into biological systems. Successful qPCR requires the use of appropriate reference genes for the normalization of data. In the present study, we aimed to identify and assess the best-suited reference genes in near-isogenic resistant (R) and susceptible (S) tomato lines infected with begomovirus Tomato curly stunt virus (ToCSV). Ten candidate reference genes namely, Actin7 (ACT), β-6 Tubulin (TUB), Ubiquitin 3 (UBI), Clathrin adaptor complexes medium subunit (CAC), Phytoene desaturase (PDS), Expressed protein (EXP), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Adenine phosphoribosyl transferase-like protein (APT1), TAP42-interacting protein (TIP41) and Elongation factor 1-alpha (EF1α) were selected and evaluated for their expression stability in resistant and susceptible tomato leaves using the analytical tools geNorm, NormFinder, BestKeeper, and RefFinder. After ranking the reference genes from most to least stable, the results suggested that a combination ofACT,EXP, andEF1αin the S lines and a combination ofTIP41,APT1, andACTin the R line is appropriate for qPCR normalization. Furthermore, to validate the identified reference genes, iron superoxide dismutase (SOD), heat shock protein 70 (HSP70) and Glutathione-S-transferase (GST) were selected as targets for normalization. The relative expression of the target genes varied when normalized against the most stable reference genes in comparison to the least stable genes. These results highlight the importance of careful selection of reference genes for accurate normalization in qPCR studies.
Ultrastructure of the nebenkern during spermatogenesis in the praying mantid Hierodula membranacea
Authors: Köckert Maria , Okafornta Chukwuebuka William , Hill Charlice , Ryndyk Anne , Striese Cynthia , Müller-Reichert Thomas , Paliulis Leocadia , Fabig Gunar
Published: July 2023

Spermatogenesis leads to the formation of functional sperm cells. Here we have applied high-pressure freezing in combination with transmission electron microscopy (TEM) to study the ultrastructure of sperm development in subadult males of the praying mantid Hierodula membranacea, a species in which spermatogenesis had not previously been studied. We show the ultrastructure of different stages of sperm development in this species. Thorough examination of TEM data and electron tomographic reconstructions revealed interesting structural features of the nebenkern, an organelle composed of fused mitochondria that has been studied in spermatids of other insect species. We have applied serial-section electron tomography of the nebenkern to demonstrate in three dimensions (3D) that this organelle in H. membranacea is composed of two interwoven mitochondrial derivatives, and that the mitochondrial derivatives are connected by a zipper-like structure at opposing positions. Our approach will enable further ultrastructural analyses of the nebenkern in other organisms.
Impact of climate change on SARS-CoV-2 epidemic in China
Authors: Yu Zhenyu , Wang Jinnian , Tan Zixuan , Luo Yiyun
Published: July 2023

The outbreak and prevalence of SARS-CoV-2 have severely affected social security. Physical isolation is an effective control that affects the short-term human-to-human transmission of the epidemic, although weather presents a long-term effect. Understanding the effect of weather on the outbreak allow it to be contained at the earliest possible. China is selected as the study area, and six weather factors that receive the most attention from January 20, 2020 to April 30, 2020 are selected to investigate the correlation between weather and SARS-CoV-2 to provide a theoretical basis for long-term epidemic prevention and control. The results show that (1) the average growth rate (GR) of SARS-CoV-2 in each province is logarithmically distributed with a mean value of 5.15%. The GR of the southeastern region is higher than that of the northwestern region, which is consistent with the Hu Line. (2) The specific humidity, 2-m temperature (T), ultraviolet (UV) radiation, and wind speed (WS) adversely affect the GR. By contrast, the total precipitation (TP) and surface pressure (SP) promote the GR. (3) For every 1 unit increase in UV radiation, the GR decreases by 0.30% in 11 days, and the UV radiation in China is higher than that worldwide (0.92% higher per day). Higher population aggregation and urbanization directly affect the epidemic, and weather is an indirect factor.
Weight stigma after bariatric surgery: A qualitative study with Brazilian women
Authors: Dimitrov Ulian Mariana , Fernandez Unsain Ramiro , Rocha Franco Ruth , Aurélio Santo Marco , Brewis Alexandra , Trainer Sarah , SturtzSreetharan Cindi , Wutich Amber , Gualano Bruno , Baeza Scagliusi Fernanda
Published: July 2023

Prior studies suggest that one anticipated benefit of bariatric surgery is the achievement of a thinner body, one that is less subject to perceived negative judgment and condemnation by others. However, additional analyses also indicate that stigma may persist even with significant post-surgery weight loss. To investigate the stigma-related perceptions and experiences of women who have undergone bariatric surgery and the resulting body transformations, we conducted individual, semi-structured interviews with thirty Brazilian women (15 aged 33–59 and 15 aged 63–72). The resulting text was then analyzed using thematic analysis. We found that some form of weight stigma persisted for our participants, regardless of weight loss. Ongoing experiences of stigma were also evidenced by the constant internal and external vigilance reported by the women, as well as their articulated efforts to distance themselves from their previous bodies. Additionally, participants reported being judged for choosing an “easy way out” to lose weight. Those in the older group reported that weight stigma was entangled with ageism: older participants received mixed messages underscoring the ways that weight and age may interact in doubly stigmatizing ways. Family and close peers were especially powerful sources of stigma experiences. Collectively, these results show that weight stigma persists even when people undergo a procedure to lose substantive weight and that the degree and types of stigma experiences are influenced by gender and age. Our study suggest future research should explore whether a targeted approach might be more effective, for example, an approach that would emphasize the importance of developing coping strategies with respect to experiences of stigma and discrimination after surgery.
A comprehensive protein interaction map and druggability investigation prioritized dengue virus NS1 protein as promising therapeutic candidate
Authors: Farooq Qurrat ul Ain , Aiman Sara , Ali Yasir , Shaukat Zeeshan , Ali Yasir , Khan Asifullah , Samad Abdus , Wadood Abdul , Li Chunhua
Published: July 2023

Dengue Virus (DENV) is a serious threat to human life worldwide and is one of the most dangerous vector-borne diseases, causing thousands of deaths annually. We constructed a comprehensive PPI map of DENV with its host Homo sapiens and performed various bioinformatics analyses. We found 1195 interactions between 858 human and 10 DENV proteins. Pathway enrichment analysis was performed on the two sets of gene products, and the top 5 human proteins with the maximum number of interactions with dengue viral proteins revealed noticeable results. The non-structural protein NS1 in DENV had the maximum number of interactions with the host protein, followed by NS5 and NS3. Among the human proteins, HBA1 and UBE2I were associated with 7 viral proteins, and 3 human proteins (CSNK2A1, RRP12, and HSP90AB1) were found to interact with 6 viral proteins. Pharmacophore-based virtual screening of millions of compounds in the public databases was performed to identify potential DENV-NS1 inhibitors. The lead compounds were selected based on RMSD values, docking scores, and strong binding affinities. The top ten hit compounds were subjected to ADME profiling which identified compounds C2 (MolPort-044-180-163) and C6 (MolPort-001-742-737) as lead inhibitors against DENV-NS1. Molecular dynamics trajectory analysis and intermolecular interactions between NS1 and the ligands displayed the molecular stability of the complexes in the cellular environment. The in-silico approaches used in this study could pave the way for the development of potential specie-specific drugs and help in eliminating deadly viral infections. Therefore, experimental and clinical assays are required to validate the results of this study.
Impact of changes in chatbot’s facial expressions on user attention and reaction time
Authors: Bortko Kamil , Fornalczyk Kacper , Jankowski Jarosław , Sulikowski Piotr , Dziedziak Karina
Published: July 2023

Communication within online platforms supported by chatbots requires algorithms, language processing methods, and an effective visual representation. These are crucial elements for increasing user engagement and making communication more akin to natural conversation. Chatbots compete with other graphic elements within websites or applications, and thus attracting a user’s attention is a challenge even before the actual conversation begins. A chatbot may remain unnoticed even with sophisticated techniques at play. Drawing attention to the chatbot area localized within the periphery area can be carried out with the use of various visual characteristics. The presented study analyzed the impact of changes in a chatbot’s emotional expressions on user reaction. The aim of this study was to observe, based on user reaction times, whether changes in a chatbot’s emotional expressions make it more noticeable. The results showed that users are more sensitive to positive emotions within chatbots, as positive facial expressions were noticed more quickly than negative ones.
M2b macrophages protect against doxorubicin induced cardiotoxicity via alternating autophagy in cardiomyocytes
Authors: Chen Sida , Huang Yang , Huang Suiqing , Zhou Zhuoming , Liu Kaizheng , Pan Jinyu , Wu Zhongkai
Published: July 2023

Objective

Doxorubicin (DOX) is an anthracycline antibiotic which is widely used for the treatment of various cancers, while the dose-related cardiotoxicity limits its potential therapeutic application. The underlying mechanism of DOX induced cardiotoxicity is complex and remains elusive. Our previous studies have shown that M2b macrophage plays an important role in reducing inflammation due to ischemic reperfusion injury in the myocardium. The purpose of this study was to investigate the potential protective role of M2b macrophages in DOX induced cardiotoxicity.

Methods

In vivo, we conducted DOX induced cardiac injury in C57BL/6 mice and treated them with M2b macrophages. Then, the mice were examined by echocardiography. The heart specimens were harvested for histological examination, transmission electron microscope analysis, and autophagy molecules evaluation. In vitro, HL-1 cardiac cell lines treated with DOX were cocultured with or without M2b macrophages. Then, Autophagy related genes and protein expression were assessed by real-time quantitative PCR and western blot; cell proliferation was assessed by cell counting kit-8.

Results

We found that M2b macrophages can improve cardiac function and alleviate cardiac injury in DOX induced cardiac injury mice. M2b macrophages can enhance cardiac autophagy levels both in vivo and in vitro in DOX induced cardiac injury model. In addition, this protective effect can be blocked by an autophagy inhibitor.

Conclusion

Our study shows that M2b macrophages can help attenuate the DOX induced cardiotoxicity by regulating the autophagy level of cardiomyocytes.
Participatory action research to address lack of safe water, a community-nominated health priority in rural South Africa
Authors: Hove Jennifer , Mabetha Denny , van der Merwe Maria , Twine Rhian , Kahn Kathleen , Witter Sophie , D’Ambruoso Lucia
Published: July 2023

Background

Despite international evidence supporting community participation in health for improved health outcomes and more responsive and equitable health systems there is little practical evidence on how to do this. This work sought to understand the process involved in collective implementation of a health-related local action plan developed by multiple stakeholders.

Methods

Communities, government departments and non-government stakeholders convened in three iterative phases of a participatory action research (PAR) learning cycle. Stakeholders were involved in problem identification, development, and implementation of a local action plan, reflection on action, and reiteration of the process. Participants engaged in reflective exercises, exploring how factors such as power and interest impacted success or failure.

Results

The local action plan was partially successful, with three out of seven action items achieved. High levels of both power and interest were key factors in the achievement of action items. For the achieved items, stakeholders reported that continuous interactions with one another created a shift in both power and interest through ownership of implementation processes. Participants who possessed significant power and influence were able to leverage resources and connections to overcome obstacles and barriers to progress the plan. Lack of financial support, shifting priorities and insufficient buy-in from stakeholders hindered implementation.

Conclusion

The process offered new ways of thinking and stakeholders were supported to generate local evidence for action and learning. The process also enabled exploration of how different stakeholders with different levels of power and interest coalesce to design, plan, and act on evidence. Creation of safe spaces was achievable, meanwhile changing stakeholders’ level of power and interest was possible but challenging. This study suggests that when researchers, service providers and communities are connected as legitimate participants in a learning platform with access to information and decision-making, a shift in power and interest may be feasible.
Assessing the knowledge and attitude towards HIV/AIDS among the general population and health care professionals in MENA region
Authors: Elghazaly Ayman , AlSaeed Nawaf , Islam Syed , Alsharif Ibtihaj , Alharbi Layla , Al Ashagr Tala , Alshanifi Albatoul , Alrashoudi Reem , Alsharidi Aynaa , Alhokail Abdullah , Dirar Qais , Shibl Atif , Al-Kattan Khaled , Abothneen Noura , Al-Mozaini Maha
Published: July 2023

Introduction

Human Immunodeficiency Virus infection continue to represent a global health concern influenced by various social, economic, and cultural factors. The MENA are among the top regions in the world with the fastest-growing HIV epidemic. Thus, adequate knowledge and a positive attitude of people toward HIV/AIDS are of utmost importance to prevent the spreading of the disease. Accordingly, this study aims to evaluate the knowledge and attitude of the public and healthcare population toward HIV/AIDs.

Methods

A cross-sectional analysis was conducted among residents within our population from October 2018 until August 2019. An anonymous online questionnaire was used to investigate the population’s demographic characteristics, HIV/AIDS-related knowledge, and attitudes toward HIV-infected patients. Participants completed a 40-item questionnaire designed to measure their knowledge and attitude toward HIV/AIDS. The data was collected via surveys, administered through electronic tablets to the participants at public places (n = 5,757) and through an online version of the questionnaire on Google Forms (n = 2500), which was sent through social media platforms. Descriptive statistics were used to analyse the data using the R-statistical software program.

Results

A total of 8,257 participants were included in our analysis. Saudi Arabian citizens represented 79% of the participants, while participants from the MENA countries represented 11.7% and 3% from the other Gulf Cooperation Council countries. Fifty-nine (59%) knew that HIV is a contagious infection, and 13.8% were unaware that HIV could be transmitted sexually. A few healthcare professionals reported negative attitudes toward HIV infected patients. Many risk factors, including age, gender, nationality, and education, significantly affected the knowledge and attitude scores. In this survey, we found that social media is the primary source of participants’ information.

Conclusions

Overall correct knowledge score of individuals about HIV/AIDS was relatively low. This study showed that the general population was knowledgeable to a certain degree about HIV/AIDS and its modes of transmission. Nevertheless, they lack a detailed understanding of the disease’s nature, modes of transmission, and existing treatment. Policymakers in the region should further eliminate social discrimination and stigma in HIV-infected patients.
New failure mechanism for evaluating ultimate inclined load adjacent to slope
Authors: Fang Hongwei , Wang Ning , Xu Yixiang
Published: July 2023

A new failure mechanism is proposed for calculating the ultimate inclined load adjacent to the slope, i.e., the slope is in the limit state when the critical slope contour and the slope surface are at the critical position where two intersections will occur. The conventional view is that the critical slope contour calculated by the method of characteristics has only a concave shape. This study found that the critical slope contour changes from concave to convex when the inclined load imposed on the slope top surface increases. The feasibility of the proposed method is verified by the finite element limit analysis (FELA) and the definition of the ultimate load. The parametric analysis showed that the current method of characteristics (CMOC) overestimated the ultimate inclined load and gave an incorrect conclusion since it assumed larger failure models at a low strength ratio or large friction angle. The proposed method does not require assumption or search of the failure models, and it can solve the shortcomings of CMOC.
Protocol for a pilot randomized controlled trial of a telehealth-delivered counseling intervention to reduce suicidality and improve HIV care engagement in Tanzania
Authors: Knettel Brandon A. , Knippler Elizabeth T. , Amiri Ismail , Joel Louise , Madundo Kim , Msoka Elizabeth F. , Boshe Judith , Tarimo Clotilda S. , Katiti Victor , Rwakilomba Jackline , Turner Elizabeth L. , Minja Linda , Staton Catherine A. , Vissoci Joao Ricardo N. , Mmbaga Blandina T. , Relf Michael V. , Goldston David B.
Published: July 2023

Objective

Suicidal ideation is strikingly common among people living with HIV (PLWH) worldwide, leading to higher burden of disease, poor HIV care engagement, and loss of life. In low- and middle-income countries such as Tanzania, mental health resources are scarce, requiring innovative strategies for treatment. We describe the protocol for a clinical trial of a three-session telehealth counseling intervention to reduce suicidality and improve HIV care engagement in Tanzania.

Methods

In a pilot randomized controlled trial, we will assess the feasibility, acceptability, and potential efficacy of a new telehealth intervention, termed “IDEAS for Hope”. A total of 60 PLWH will be enrolled from two HIV clinics in the Kilimanjaro region and connected to telehealth counsellors based at a large regional hospital. Participants will be ≥18 years old and speak either Kiswahili or English. Patient screening will occur during routine HIV clinical care to identify PLWH experiencing suicidal ideation. Baseline surveys will be administered upon enrollment and participants will be randomized 1:1 to receive either IDEAS for Hope or the comparison condition, a brief safety planning session. All participants will receive an additional referral for psychiatric treatment. Follow-up assessment will occur at three months. IDEAS for Hope is informed by a Motivational Interviewing-enhanced safety planning intervention (MI-SafeCope) and our formative work in Tanzania. The model consists of Four Pillars: living healthy with HIV, managing HIV stigma, seeking social support, and meeting basic needs. Together, these mechanisms serve as a foundation for developing a sense of safety and hope for the future. Outcome measures will include intervention feasibility, acceptability, participant suicidality, and HIV care engagement.

Significance

Innovative, telehealth-based counseling represents a promising treatment for suicidality among PLWH in low-resource settings. Results from this pilot trial will inform intervention refinement and parameter estimates for a future clinical trial powered to evaluate effectiveness.
Effects of an academic detailing service on benzodiazepine prescribing patterns in primary care
Authors: Lacroix Meagan , Abdelmalek Fred , Everett Karl , Salach Lena , Bevan Lindsay , Burton Victoria , Ivers Noah M. , Tadrous Mina
Published: July 2023

Background

Benzodiazepines are commonly used to treat anxiety and/or insomnia but are associated with substantial safety risks. Changes to prescribing patterns in primary care may be facilitated through tailored quality improvement strategies. Academic detailing (AD) may be an effective method of promoting safe benzodiazepine prescribing. The objective of this study was to evaluate the effectiveness of AD on benzodiazepine prescribing among family physicians.

Methods and findings

We used an interrupted time series matched cohort design using population-based administrative claims databases. Participants were family physicians practicing in Ontario, Canada. The intervention was a voluntary AD service which involves brief service-oriented educational outreach visits by a trained pharmacist. The focus was on key messages for safer benzodiazepine prescribing in primary care with an emphasis on judicious prescribing to older adults aged 65 and older. Physicians in the intervention group were those who received at least one AD visit on benzodiazepine use between June 2019 and February 2020. Physicians in the control group were included if they did not receive an AD visit during the study period. Intervention physicians were matched to control physicians 1:4, on a variety of characteristics. Physicians were excluded if they had inactive billing or billing of less than 100 unique patient visits in the calendar year prior to the index date. The primary outcome was mean total benzodiazepine prescriptions at the level of the physician. Secondary outcomes were rate (per 100) of patients with long-term prescriptions, high-risk prescriptions, newly started prescriptions, and benzodiazepine-related patient harms. Data were analyzed using a repeated measures pre-post comparison with an intention-to-treat. Analyses were then stratified to focus on effects within higher-prescribing physicians.

There were 1337 physicians were included in the study; 237 who received AD and 1064 who did not. There was no significant change in benzodiazepine prescribing when considering all physicians in the intervention and matched control groups. Although not significant, a greater reduction in total benzodiazepine prescriptions was observed amongst the highest-volume prescribing physicians who received the intervention (% change in slope = -0.53, 95%CI = -2.34 to 1.30, p > .05).

The main limitation of our study was the voluntary nature of the AD intervention, which may have introduced a self-selection bias of physicians most open to changing their prescribing.

Conclusion

This study suggests that future AD interventions should focus on physicians with the greatest room for improvement to their prescribing.
Regulation of the phagocytic activity of astrocytes by neuroimmune mediators endogenous to the central nervous system
Authors: Yang Sijie (Shirley) , Simtchouk Svetlana , Gibon Julien , Klegeris Andis
Published: July 2023

The phagocytic activity of glial cells is essential for maintaining normal brain activity, and its dysfunction may contribute to the central nervous system (CNS) pathologies, including neurodegenerative diseases. Phagocytic activity is one of the well-established neuroimmune functions of microglia. Although emerging evidence indicates that astrocytes can also function as CNS phagocytes in humans and rodents, limited information is available about the molecular mechanism regulating this function. To address this knowledge gap, we studied modulation of the phagocytic activity of human U118 MG astrocytic cells and murine primary astrocytes by four CNS inflammatory mediators and bacterial endotoxin lipopolysaccharide (LPS). LPS and cytochrome c (CytC) upregulated, while interferon (IFN)-γ downregulated, phagocytosis of latex beads by human astrocytic cells and phagocytosis of synaptosomes by murine primary astrocytes. Interleukin (IL)-1β and tumor necrosis factor (TNF)-α had no effect on the phagocytic activity of human astrocytic cells but upregulated this function in murine astrocytes. Varying effects of combinations of the above inflammatory mediators were observed in these two cell types. LPS- and CytC-induced phagocytic activity of human astrocytic cells was partially mediated by activation of toll-like receptor 4 (TLR4). By monitoring other functions of astrocytes, we concluded there were no correlations between the effects of the mediators studied on astrocyte phagocytic activity and their secretion of cytokines, cytotoxins, or glutamate. Our study identified four candidate CNS regulators of astrocyte phagocytic activity. Future investigation of molecular mechanisms behind this regulation could identify novel therapeutic targets allowing modulation of this astrocyte-mediated clearance mechanism in CNS pathologies.
Social support for postpartum women and associated factors including online support to reduce stress and depression amidst COVID-19: Results of an online survey in Thailand
Authors: Kim Soo Jung , Aye Yin Min , Panyarachun Danipa , Hong Seo Ah , Chang Yan-Shing
Published: July 2023

Background

Social support for postpartum women helps mothers to recover from childbirth and fosters healthy infant development. However, the impacts of reduced interpersonal interactions inflicted by the COVID-19 outbreak on available social support for postpartum women have received little attention. Therefore, this study aimed to examine the levels of social support provided to postpartum women and associated factors in Thailand during the COVID-19 pandemic.

Methods

A cross-sectional study was conducted from July to October 2021 using an anonymous online questionnaire. The responses of 840 eligible women up to six months postpartum in Thailand were obtained. The maternity social support scale was used to measure social support. Multivariate logistic regression was used to analyse the factors associated with social support among postpartum women.

Results

About 57% of women reported to receive high support. Women in the high social support group were more likely to be married (aOR:2.70; 95% CI:1.57–4.66), have a university education or above (1.88; 1.35–2.64), have an intended pregnancy (2.06; 1.34–3.16), good health (2.01; 1.44–2.81), good sleep quality (1.62; 1.14–2.31), receive counsel from peers or family (1.56; 1.13–2.16), and use internet or social media to reduce stress and depression (1.51; 1.08–2.11). Meanwhile, women in the high social support group were significantly less likely to feed complementary foods to infants within 24 hours of completing the survey (0.28; 0.15–0.52).

Conclusions

The results of this study indicated that more than half of the women reported high support and illustrated the important role played by family, peers, and professionals as well as online and remote channels in providing postpartum informational and emotional support during the pandemic. Online platforms and remote support may be considered to provide social support to postpartum women during a pandemic such as COVID-19.
Utilization of diabetes self-management program among breast, prostate, and colorectal cancer survivors: Using 2006–2019 Texas Medicare data
Authors: Lee Wei-Chen , Digbeu Biai Dominique Elmir , Serag Hani , Sallam Hanaa , Kuo Yong-Fang
Published: July 2023

Background

Cancer treatment is associated with inferior health outcomes such as diabetes. Medicare provides Diabetes Self-Management Training (DSMT) program to beneficiaries to achieve normal metabolic control and reduce the risk of micro and macro-vascular complications. This study aimed to examine the trend of DSMT utilization among cancer survivors and assess individual characteristics associated with it.

Methods

The data for this study was from Texas Cancer Registry-Medicare linkage data of patients with prostate, breast, or colorectal cancer diagnosed in 1999–2017. Outcome variables include the number of first-time DSMT users, the number of total users, and the average number of DSMT utilization in minutes. We performed logistic regression and gamma regression to obtain a multivariable-adjusted odds ratio for the association between DSMT utilization and individual characteristics.

Results

The number of first-time users has slowly increased over the years (from 99 to 769 per 1,000) but suddenly dropped after 2016. The number of all users (first-time and follow-up users) has increased (from 123 to 1,201 per 1,000) and plateaued after 2016. Determinants including Hispanic ethnicity (O.R. = 1.10) and Medicare-Medicaid dual eligibility (O.R. = 1.25) are positively associated with both the initiation and retention of the DSMT. A barrier to both initiation and retention of DSMT is living in a metropolitan area (O.R. = 0.90).

Conclusions

Multi-level strategies to enhance accessibility and availability of DSMT programs for Medicare beneficiaries are highly recommended. Examining the determinants of initiation and retention of DSMT over 14 years provides insights on strategies to meet the needs of cancer survivors and reduce the burden of diabetes on them.