bluuebunny/crossref_metadata_2025 huggingface dataset:
Idiopathic Non-Dental Facial Pain Syndromes in Italian Children: A Clinical Case Series
Authors: Correnti Edvige , Lo Cascio Salvatore , Cernigliaro Federica , Rossi Roberta , D’Agnano Daniela , Grasso Giulia , Pellegrino Annamaria , Lauria Barbara , Santangelo Andrea , Santangelo Giuseppe , Tripi Gabriele , Versace Antonella , Sciruicchio Vittorio , Raieli Vincenzo
Published: March 2023

Background. The orofacial pain syndromes (OFPs) are a heterogeneous group of syndromes characterized by painful attacks involving the orofacial structures. They may be summarily subdivided into two great categories: (1) orofacial pain mainly attributed to dental disorders such as dentoalveolar and myofascial orofacial pain or temporomandibular joint (TM) pain; (2) orofacial pain mainly attributed to non-dental pain as neuralgias, facial localization of primary headaches or idiopathic orofacial pain. The second group is uncommon, often described by single case reports, can often show overlapping symptoms with the first group, and represents a clinical challenge, carrying the risk of undervaluation and possibly invasive odontoiatric treatment. We aimed to describe a clinical pediatric series of non-dental orofacial pain and better to underline some topographic and clinical features associated with them. We retrospectively collected the data of children admitted to our headache centers (Bari, Palermo, Torino) from 2017 to 2021. Our inclusion criterion was the presence of non-dental orofacial pain following the topographic criteria of 3° International Classification of Headache Disorders (ICHD-3), and exclusion criteria included the pain syndromes attributed to the dental disorders and pain syndromes due to the secondary etiologies Results. Our sample comprised 43 subjects (23/20 M/F, in the range of ages 5–17). We classified them int: 23 primary headaches involving the facial territory during attacks, 2 facial trigeminal autonomic cephalalgias, 1 facial primary stabbing headache, 1 facial linear headache, 6 trochlear migraines, 1 orbital migraine 3 red ear syndrome and 6 atypical facial pain. All patients described debilitating pain for intensity (moderate/severe), 31 children had episodic attacks, and 12 had continuous pain. Almost all received drugs for acute treatment (less than 50% were satisfied), and some received non-pharmacological treatment associated with drug therapy Conclusion. Although rare OFP can occur in pediatric age, it can be debilitating if unrecognized and untreated, affecting the psychophysical well-being of young patients. We highlight the specific characteristics of the disorder for a more correct and earlier identification during the diagnostic process, already difficult in pediatric age, and to define the approach and possible treatment to prevent negative outcomes in adulthood.
Carbon Biogeochemistry of the Estuaries Adjoining the Indian Sundarbans Mangrove Ecosystem: A Review
Authors: Das Isha , Chanda Abhra , Akhand Anirban , Hazra Sugata
Published: March 2023

The present study reviewed the carbon-biogeochemistry-related observations concerning CO2 and CH4 dynamics in the estuaries adjoining the Indian Sundarbans mangrove ecosystem. The review focused on the partial pressure of CO2 and CH4 [pCO2(water) and pCH4(water)] and air–water CO2 and CH4 fluxes and their physical, biogeochemical, and hydrological drivers. The riverine-freshwater-rich Hooghly estuary has always exhibited higher CO2 emissions than the marine-water-dominated Sundarbans estuaries. The mangrove sediment porewater and recirculated groundwater were rich in pCO2(water) and pCH4(water), enhancing their load in the adjacent estuaries. Freshwater-seawater admixing, photosynthetically active radiation, primary productivity, and porewater/groundwater input were the principal factors that regulated pCO2(water) and pCH4(water) and their fluxes. Higher chlorophyll-a concentrations, indicating higher primary production, led to the furnishing of more organic substrates that underwent anaerobic degradation to produce CH4 in the water column. The northern Bay of Bengal seawater had a high carbonate buffering capacity that reduced the pCO2(water) and water-to-air CO2 fluxes in the Sundarbans estuaries. Several authors traced the degradation of organic matter to DIC, mainly following the denitrification pathway (and pathways between aerobic respiration and carbonate dissolution). Overall, this review collated the significant findings on the carbon biogeochemistry of Sundarbans estuaries and discussed the areas that require attention in the future.
The Use of Medicinal Plants in Blood Vessel Diseases: The Influence of Gender
Authors: Froldi Guglielmina
Published: March 2023

Data available in the literature on the use of herbal products to treat inflammation-related vascular diseases were considered in this study, while also assessing the influence of gender. To this end, the articles published in PubMed over the past 10 years that described the use of plant extracts in randomized clinical trials studying the effectiveness in vascular pathologies were analyzed. The difference in efficacy of plant-derived preparations in female and male subjects was always considered when reporting. The safety profiles of the selected plants were described, reporting unwanted effects in humans and also by searching the WHO database (VigiBase®). The medicinal plants considered were Allium sativum, Campomanesia xanthocarpa, Sechium edule, Terminalia chebula. Additionally, an innovative type of preparation consisting of plant-derived nanovesicles was also reported.
The Role of the Transjugular Intrahepatic Porto-Systemic Shunt in an Emergency Setting
Authors: Posa Alessandro , Tenore Lorenzo , Barbieri Pierluigi , Mazza Giulia , Sala Evis , Iezzi Roberto
Published: March 2023

Transjugular intrahepatic porto-systemic shunt (TIPSS) is an interventional radiology procedure whose aim is to create artificial communication between the portal and the hepatic blood flow in order to reduce the pressure gradient in portal hypertension. The indications to perform a TIPSS procedure can be framed in an elective or emergency setting: refractory ascites to diuretic therapy and secondary prophylaxis of variceal hemorrhage are the most frequent reasons for executing a TIPSS in an election context, while acute uncontrolled variceal bleeding is the principal indication that a TIPSS needs to be performed in an emergency setting. In recent years, the role of the TIPSS has been redefined for several conditions, such as ectopic varices, portal vein thrombosis, Budd–Chiari syndrome, hepatic veno-occlusive disease, and many others. This review aims to perform a deep analysis of when and why a TIPSS procedure should be carried out in an emergency, pointing out the related most common technical difficulties and complications.
Photoluminescent Scaffolds Based on Natural and Synthetic Biodegradable Polymers for Bioimaging and Tissue Engineering
Authors: Trifanova Ekaterina M. , Babayeva Gulalek , Khvorostina Maria A. , Atanova Aleksandra V. , Nikolaeva Maria E. , Sochilina Anastasia V. , Khaydukov Evgeny V. , Popov Vladimir K.
Published: March 2023

Non-invasive visualization and monitoring of tissue-engineered structures in a living organism is a challenge. One possible solution to this problem is to use upconversion nanoparticles (UCNPs) as photoluminescent nanomarkers in scaffolds. We synthesized and studied scaffolds based on natural (collagen—COL and hyaluronic acid—HA) and synthetic (polylactic-co-glycolic acids—PLGA) polymers loaded with β-NaYF4:Yb3+, Er3+ nanocrystals (21 ± 6 nm). Histomorphological analysis of tissue response to subcutaneous implantation of the polymer scaffolds in BALB/c mice was performed. The inflammatory response of the surrounding tissues was found to be weak for scaffolds based on HA and PLGA and moderate for COL scaffolds. An epi-luminescent imaging system with 975 nm laser excitation was used for in vivo visualization and photoluminescent analysis of implanted scaffolds. We demonstrated that the UCNPs’ photoluminescent signal monotonously decreased in all the examined scaffolds, indicating their gradual biodegradation followed by the release of photoluminescent nanoparticles into the surrounding tissues. In general, the data obtained from the photoluminescent analysis correlated satisfactorily with the histomorphological analysis.
Biocatalytic Membranes for Carbon Capture and Utilization
Authors: Shen Jialong , Salmon Sonja
Published: March 2023

Innovative carbon capture technologies that capture CO2 from large point sources and directly from air are urgently needed to combat the climate crisis. Likewise, corresponding technologies are needed to convert this captured CO2 into valuable chemical feedstocks and products that replace current fossil-based materials to close the loop in creating viable pathways for a renewable economy. Biocatalytic membranes that combine high reaction rates and enzyme selectivity with modularity, scalability, and membrane compactness show promise for both CO2 capture and utilization. This review presents a systematic examination of technologies under development for CO2 capture and utilization that employ both enzymes and membranes. CO2 capture membranes are categorized by their mode of action as CO2 separation membranes, including mixed matrix membranes (MMM) and liquid membranes (LM), or as CO2 gas–liquid membrane contactors (GLMC). Because they selectively catalyze molecular reactions involving CO2, the two main classes of enzymes used for enhancing membrane function are carbonic anhydrase (CA) and formate dehydrogenase (FDH). Small organic molecules designed to mimic CA enzyme active sites are also being developed. CO2 conversion membranes are described according to membrane functionality, the location of enzymes relative to the membrane, which includes different immobilization strategies, and regeneration methods for cofactors. Parameters crucial for the performance of these hybrid systems are discussed with tabulated examples. Progress and challenges are discussed, and perspectives on future research directions are provided.
Study on the Microstructure and Mechanical Properties of Non-Equimolar NiCoFeAlTi High Entropy Alloy Doped with Trace Elements
Authors: Wu Chunfen , Zhang Shuzhi , Han Jianchao , Zhang Changjiang , Kong Fantao
Published: March 2023

The method of improving the microstructure and thus the properties of alloys by adjusting their composition has been widely used in the study of high entropy alloys (HEAs). However, most studies have focused on improving the properties of HEAs with face-centered cubic (FCC) or body-centered cubic (BCC) structures by adjusting the contents of elements such as Ni, Al, Ti, Cr, Mn and Mo. The doping of B, Mg and Zr also has a certain effect on the mechanical properties of HEAs. In this paper, the phase structure, microstructure, and mechanical properties of Ni45.5Co22Fe22Al5Ti5 HEA doped with B, Mg, and Zr were investigated. The results demonstrated that the three-phase structures of FCC matrix, L12 precipitate, and BCC phase were present in all the as-cast HEAs of Ni45.5Co22Fe22Al5Ti5×0.5 (X = B, Mg, and Zr). The microstructures of the as-cast alloys showed typical dendritic and inter-dendritic architecture. The maximum hardness was found in the alloy doped with B element, with a value of 433 HV. During the compressive test at room temperature, neither the Mg0.5 HEA nor the Zr0.5 HEA cracked until the load limit, but the B0.5 HEA cracked at a compressive strain of about 12%. B0.5 HEA had the highest compressive yield strength of the three alloys, followed by Zr0.5 HEA, while Mg0.5 HEA had the lowest, with values of 1030 MPa, 754 MPa, and 628 MPa, respectively. The work is expected to provide a boost for the research on the optimization of the properties of new HEAs reinforced by precipitation of L12 phase by providing a simple solution-microalloying method.
Glutathione for Food and Health Applications with Emphasis on Extraction, Identification, and Quantification Methods: A Review
Authors: Al-Temimi Anfal Alwan , Al-Mossawi Aum-El-Bashar , Al-Hilifi Sawsan A. , Korma Sameh A. , Esatbeyoglu Tuba , Rocha João Miguel , Agarwal Vipul
Published: March 2023

Glutathione is a naturally occurring compound that plays a crucial role in the cellular response to oxidative stress through its ability to quench free radicals, thus mitigating the risk of potential damage, including cell death. While glutathione is endogenously present in different plants and animal cells, their concentration varies considerably. The alteration in glutathione homeostasis can be used as a potential marker for human diseases. In the case of the depletion of endogenous glutathione, exogenous sources can be used to replenish the pool. To this end, both natural and synthetic glutathione can be used. However, the health benefit of glutathione from natural sources derived from fruits and vegetables is still debated. There is increasingly growing evidence of the potential health benefits of glutathione in different diseases; however, the determination and in situ quantification of endogenously produced glutathione remains a major challenge. For this reason, it has been difficult to understand the bioprocessing of exogenously delivered glutathione in vivo. The development of an in situ technique will also aid in the routine monitoring of glutathione as a biomarker for different oxidative stress-mediated diseases. Furthermore, an understanding of the in vivo bioprocessing of exogenously delivered glutathione will also aid the food industry both towards improving the longevity and profile of food products and the development of glutathione delivery products for long-term societal health benefits. In this review, we surveyed the natural plant-derived sources of glutathione, the identification and quantification of extracted glutathione from these sources, and the role of glutathione in the food industry and its effect on human health.
Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi
Authors: Piątek-Gołda Wiktoria , Sulej Justyna , Grąz Marcin , Waśko Piotr , Janik-Zabrotowicz Ewa , Osińska-Jaroszuk Monika
Published: March 2023

Enzymes produced by white rot fungi are involved in the synthesis of secondary metabolites with valuable biotechnological properties. One of these metabolites is lactobionic acid (LBA). The aim of this study was to characterize a novel enzyme system consisting of a cellobiose dehydrogenase from Phlebia lindtneri (PlCDH), a laccase from Cerrena unicolor (CuLAC), a redox mediator (ABTS or DCPIP), and lactose as a substrate. We used quantitative (HPLC) and qualitative methods (TLC, FTIR) to characterise the obtained LBA. The free radical scavenging effect of the synthesised LBA was assessed with the DPPH method. Bactericidal properties were tested against Gram-negative and Gram-positive bacteria. We obtained LBA in all the systems tested; however, the study showed that the temperature of 50 °C with the addition of ABTS was the most advantageous condition for the synthesis of lactobionic acid. A mixture with 13 mM LBA synthesised at 50 °C with DCPIP showed the best antioxidant properties (40% higher compared with the commercial reagent). Furthermore, LBA had an inhibitory effect on all the bacteria tested, but the effect was better against Gram-negative bacteria with growth inhibition no lower than 70%. Summarizing the obtained data, lactobionic acid derived in a multienzymatic system is a compound with great biotechnological potential.
Targeting Carbohydrate Mimetics of Tetrahydrofuran-Containing Acetogenins to Prostate Cancer
Authors: Periche Patricia Gonzalez , Lin Jacky , Bhupathiraju Naga V. S. D. K. , Kalidindi Teja , Johnson Delissa S. , Pillarsetty Nagavarakishore , Mootoo David R.
Published: March 2023

The high potency of the tetrahydrofuran-containing acetogenins (THF-ACGs) against a broad range of human cancer cell lines has stimulated interest in structurally simpler mimetics. In this context, we have previously reported THF-ACG mimetics in which the THF and butenolide moieties of a mono-THF-ACG were replaced with carbohydrate and thiophene residues, respectively. In the present study, towards the targeting of these carbohydrate analogues to prostate cancer (PCa), we synthesized prodrugs in which a parent thiophene or butenolide congener was conjugated through a self-immolative linker to 2-[3-(1,3-dicarboxypropyl)ureido] pentanedioic acid (DUPA), a highly specific ligand for prostate-specific membrane antigen (PSMA), which is overexpressed on prostate tumors. Both prodrugs were found to be more active against receptor positive LNCaP than receptor-negative PC-3 cells, with 2.5 and 12 times greater selectivity for the more potent thiophene analog and the less active butenolide congener, respectively. This selectivity for LNCaP over PC-3 contrasted with the behavior of the parent drugs, which showed similar or significantly higher activity for PC-3 compared to LNCaP. These data support the notion that higher activity of these DUPA-derived prodrugs against LNCaP cells is connected to their binding to PSMA and suggest that the conjugation of PSMA ligands to this family of cytotoxic agents may be effective for targeting them to PCa.
Triphenylamine-Containing Benzoic Acids: Synthesis, Liquid Crystalline and Redox Properties
Authors: Feringán Beatriz , Martínez-Bueno Alejandro , Sierra Teresa , Giménez Raquel
Published: March 2023

The synthesis, characterization and liquid crystalline and electrochemical properties of novel triarylamines, in which the triphenylamine platform is non-symmetrically modified with a 4-(6-oxyhexyloxy)benzoic acid group, are reported. Compounds show columnar liquid crystalline behavior, as confirmed through the use of polarized optical microscopy, differential scanning calorimetry and X-ray diffraction. Electrochemical properties were measured using cyclic voltammperometry, obtaining low oxidation potentials and HOMO values that were optimum for consideration as organic semiconductors in hole transport layers. In addition, the photoredox activity of one of these derivatives in dichloromethane was studied under light irradiation. A photooxidation/assembly process under white light irradiation occurs without the assistance of hydrogen bonding amide functional groups.
Diagonal Interactions between Glutamate and Arginine Analogs with Varying Side-Chain Lengths in a β-Hairpin
Authors: Li Nian-Zhi , Yu Chen-Hsu , Wu Jhuan-Yu , Huang Shing-Jong , Huang Shou-Ling , Cheng Richard P.
Published: March 2023

Cross-strand interactions are important for the stability of β-sheet structures. Accordingly, cross-strand diagonal interactions between glutamate and arginine analogs with varying side-chain lengths were studied in a series of β-hairpin peptides. The peptides were analyzed by homonuclear two-dimensional nuclear magnetic resonance methods. The fraction folded population and folding free energy of the peptides were derived from the chemical shift data. The fraction folded population trends could be rationalized using the strand propensity of the constituting residues, which was not the case for the peptides with lysine analogs, highlighting the difference between the arginine analogs and lysine analogs. Double-mutant cycle analysis was used to derive the diagonal ion-pairing interaction energetics. The most stabilizing diagonal cross-strand interaction was between the shortest residues (i.e., Asp2–Agp9), most likely due to the least side-chain conformational penalty for ion-pair formation. The diagonal interaction energetics in this study involving the arginine analogs appears to be consistent with and extend beyond our understanding of diagonal ion-pairing interactions involving lysine analogs. The results should be useful for designing β-strand-containing molecules to affect biological processes such as amyloid formation and protein-protein interactions.
Integrated Approach for Synthetic Cathinone Drug Prioritization and Risk Assessment: In Silico Approach and Sub-Chronic Studies in Daphnia magna and Tetrahymena thermophila
Authors: Pérez-Pereira Ariana , Carvalho Ana Rita , Carrola João Soares , Tiritan Maria Elizabeth , Ribeiro Cláudia
Published: March 2023

Synthetic cathinones (SC) are drugs of abuse that have been reported in wastewaters and rivers raising concern about potential hazards to non-target organisms. In this work, 44 SC were selected for in silico studies, and a group of five emerging SC was prioritized for further in vivo ecotoxicity studies: buphedrone (BPD), 3,4-dimethylmethcathinone (3,4-DMMC), butylone (BTL), 3-methylmethcathinone (3-MMC), and 3,4-methylenedioxypyrovalerone (MDPV). In vivo short-term exposures were performed with the protozoan Tetrahymena thermophila (28 h growth inhibition assay) and the microcrustacean Daphnia magna by checking different indicators of toxicity across life stage (8 days sublethal assay at 10.00 µg L−1). The in silico approaches predicted a higher toxic potential of MDPV and lower toxicity of BTL to the model organisms (green algae, protozoan, daphnia, and fish), regarding the selected SC for the in vivo experiments. The in vivo assays showed protozoan growth inhibition with MDPV > BPD > 3,4-DMMC, whereas no effects were observed for BTL and stimulation of growth was observed for 3-MMC. For daphnia, the responses were dependent on the substance and life stage. Briefly, all five SC interfered with the morphophysiological parameters of juveniles and/or adults. Changes in swimming behavior were observed for BPD and 3,4-DMMC, and reproductive parameters were affected by MDPV. Oxidative stress and changes in enzymatic activities were noted except for 3-MMC. Overall, the in silico data agreed with the in vivo protozoan experiments except for 3-MMC, whereas daphnia in vivo experiments showed that at sublethal concentrations, all selected SC interfered with different endpoints. This study shows the importance to assess SC ecotoxicity as it can distress aquatic species and interfere with food web ecology and ecosystem balance.
Multiple Fingerprint–Activity Relationship Assessment of Immunomodulatory Polysaccharides from Ganoderma lucidum Based on Chemometric Methods
Authors: Liu Jing , Zhang Jingsong , Feng Jie , Tang Chuanhong , Yan Mengqiu , Zhou Shuai , Chen Wanchao , Wang Wenhan , Liu Yanfang
Published: March 2023

Polysaccharides with molecular weights ranging from 1.75 × 103 to 1.14 × 104 g/mol were obtained from the fruit bodies of Ganoderma lucidum. The multiple fingerprints and macrophage immunostimulatory activity of these fractions were analyzed as well as the fingerprint–activity relationship. The correlation analysis of molecular weight and immune activity demonstrated that polysaccharides with molecular weights of 4.27 × 103~5.27 × 103 and 1 × 104~1.14 × 104 g/mol were the main active fractions. Moreover, the results showed that galactose, mannose, and glucuronic acid were positively related to immunostimulatory activity. Additionally, partial least-squares regression and grey correlation degree analyses indicated that three peaks (P2, P3, P8) in the oligosaccharide fragment fingerprint significantly affected the immune activity of the polysaccharides. Hence, these ingredients associated with activity could be considered as markers to assess Ganoderma lucidum polysaccharides and their related products, and the study also provides a reference for research on the spectrum–effect relationship of polysaccharides in the future.
Echinophora tenuifolia subsp. sibthorpiana—Study of the Histochemical Localization of Essential Oil
Authors: Ivanova Stanislava , Dyankov Stanislav , Karcheva-Bahchevanska Diana , Todorova Velislava , Georgieva Yoana , Benbassat Niko , Ivanov Kalin
Published: March 2023

Background: Echinophora tenuifolia L. subsp. sibthorpiana is a perennial, aromatic plant used in traditional folk medicine and cuisine of the Mediterranean and the Middle East. However, scholars have not fully studied the pharmacological potential of the herb, and the scientific data on this plant species are limited. This study aimed to evaluate the chemical composition of the essential oil (EO) obtained from the aerial parts of E. tenuifolia subsp. sibthorpiana growing wild in Bulgaria and to perform histochemical analysis. Methods: A microscopic histochemical analysis and gas chromatography with mass spectrometry were performed. Results: The histochemical analysis confirmed the presence of terpenes in the stem and leaf of E. tenuifolia subsp. sibthorpiana. The phenylpropanoid methyleugenol was identified as the main compound in the EO, representing 48.13% of the total oil composition. There were also found considerable amounts of monoterpene hydrocarbons, representing 41.68% of the total EO. Alpha-phellandrene, o-cymene, and β-phellandrene were the most abundant monoterpene hydrocarbons. Conclusion: This is the first histochemical analysis performed on E. tenuifolia subsp. sibthorpiana. This is the first report of the EO composition from Bulgarian E. tenuifolia subsp. sibthorpiana, and our results indicate some future possibilities for evaluating of the biological activity of the EO of E. tenuifolia subsp. sibthorpiana and highlight the potential future use of the EO of this plant species. E. tenuifolia L. subsp. sibthorpiana EO possesses a good potential for use as a biopesticide and repellent an environmentally friendly alternative of synthetic pesticides.
Polyacrylamide-Based Block Copolymer Bearing Pyridine Groups Shows Unexpected Salt-Induced LCST Behavior
Authors: Tu Yunyun , Fang Dandan , Zhan Wanli , Wei Zengming , Yang Liming , Shao Penghui , Luo Xubiao , Yang Guang
Published: March 2023

Thermal-responsive block copolymers are a special type of macromolecule that exhibit a wide range of applications in various fields. In this contribution, we report a new type of polyacrylamide-based block copolymer bearing pyridine groups of polyethylene glycol-block-poly(N-(2-methylpyridine)-acrylamide; Px) that display distinct salt-induced lower critical solution temperature (LCST) behavior. Unexpectedly, the phase-transition mechanism of the salt-induced LCST behavior of Px block copolymers is different from that of the reported LCST-featured analogues. Moreover, their thermo-responsive behavior can be significantly regulated by several parameters such as salt species and concentration, urea, polymerization degree, polymer concentration and pH values. This unique thermal behavior of pyridine-containing block copolymers provides a new avenue for the fabrication of smart polymer materials with potential applications in biomedicine.
Study of Water Freezing in Low-Fat Milky Ice Cream with Oat β-Glucan and Its Influence on Quality Indicators
Authors: Buniowska-Olejnik Magdalena , Mykhalevych Artur , Polishchuk Galyna , Sapiga Victoria , Znamirowska-Piotrowska Agata , Kot Anna , Kamińska-Dwórznicka Anna
Published: March 2023

The work is devoted to the study of the functional and technological properties of oat β-glucan in low-fat milky ice cream (2% fat) in comparison with the stabilization system Cremodan® SI 320. β-glucan (0.5%) has a greater effect on the cryoscopic temperature of ice cream mixes than Cremodan® SI 320 in the same amount (decrease by 0.166 °C vs. 0.078 °C), which inhibits the freezing process of free water in ice cream during technological processing in the temperature range from −5 to −10 °C. Microscopy of ice cream samples after freezing and hardening shows the ability of β-glucan to form a greater number of energy bonds due to specific interaction with milk proteins. Analysis of the microstructure of ice cream samples during 28 d of storage confirms the ability of oat β-glucan to suppress the growth of ice crystals more effectively than Cremodan® SI 320. Oat β-glucan gives ice cream a rich creamy taste, increases overrun and resistance to melting, which brings this type of frozen dessert closer to a full-fat analogue (10% fat).
An Experimental Approach to Address the Functional Relationship between Antioxidant Enzymes and Mitochondrial Respiratory Complexes
Authors: Mendes Daniela , Silva Ana Maria , Oliveira Maria Manuel , Andrade Paula B. , Videira Romeu A.
Published: March 2023

Mitochondrial dysfunction and cytosolic oxidative stress are pathological biomarkers interlinked in several chronic diseases and cellular toxicity promoted by high-energy radiation or xenobiotics. Thus, assessing the activities of the mitochondrial redox chain complexes and the cytosolic antioxidant enzymes in the same cell culture system is a valuable approach to addressing the challenge of chronic diseases or unveiling the molecular mechanisms underlying the toxicity of physical and chemical stress agents. The present article gathers the experimental procedures to obtain, from isolated cells, a mitochondria-free cytosolic fraction and a mitochondria-rich fraction. Furthermore, we describe the methodologies to evaluate the activity of the main antioxidant enzymes in the mitochondria-free cytosolic fraction (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase), and the activity of the individual mitochondrial complexes I, II and IV, as well as the conjugated activity of complexes I–III and complexes II–III in the mitochondria-rich fraction. The protocol to test the citrate synthase activity was also considered and used to normalize complexes. The procedures were optimized within an experimental setup to allow that each condition to be tested only requires sampling of one T-25 flask of cells 2D cultured, as the typical results presented and discussed here.
Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
Authors: Garige Mamatha , Ghosh Susmita , Roelofs Brian , Rao V. Ashutosh , Sourbier Carole
Published: March 2023

Insulin is a hormone produced by β-cells of the pancreas and controls the amount of sugar in the blood. Since its discovery over 100 years ago, insulin has been used as a life-saving treatment for people with diabetes. Historically, the biological activity or bioidentity of insulin products has been assessed using an in vivo model. However, reduction in animal experiments is a goal for many worldwide, and there is a need to develop in vitro bioassays to reliably test the biological activity of insulin products. This article describes an in vitro cell-based method to assess the biological activity of insulin glargine, insulin aspart, and insulin lispro in a step-by-step manner.
Efficient Removal of Congo Red, Methylene Blue and Pb(II) by Hydrochar–MgAlLDH Nanocomposite: Synthesis, Performance and Mechanism
Authors: Huang Yang , Yin Wei , Zhao Tian-Lei , Liu Meng , Yao Qi-Zhi , Zhou Gen-Tao
Published: March 2023

Organic dyes and heavy metals often coexist in industrial effluents, and their simultaneous removal is a grand challenge. Herein, a hydrochar and MgAl layered double hydroxide (HC–MgAlLDH) nanocomposite was prepared via a facile one-step hydrothermal route, and applied to remove anionic Congo red (CR), cationic Methylene blue (MB) and Pb(II) from aqueous solutions. The nanocomposite was formed by interweaving amorphous HC and crystalline MgAlLDH nanoplates and possessed more functional groups, lower zeta potential and larger specific surface area than uncomposited MgAlLDH. Batch removal experiments showed that the components HC and LDH dominated the CR and MB removals, respectively, whereas Pb(II) removal was conjointly controlled by the two components. The maximum Langmuir removal capacities of the nanocomposite to sole CR, MB, or Pb(II) were 348.78, 256.54 or 33.55 mg/g. In binary and ternary systems, the removal capacities of CR and MB only slightly decreased, while the capacity of Pb(II) increased by 41.13–88.61%. The increase was related to the coordination of Pb(II) with the sulfur-containing groups in dyes and the precipitation of PbSO4. Therefore, the simultaneous removal of CR, MB and Pb(II) was involved in a synergistic effect, including electrostatic adsorption, π–π interaction, coordination and precipitation. The present work shows that the HC–MgAlLDH nanocomposite has great potential for wastewater integrative treatment.
Exceptional Sorption of Heavy Metals from Natural Water by Halloysite Particles: A New Prospect of Highly Efficient Water Remediation
Authors: Stor Michał , Czelej Kamil , Krasiński Andrzej , Gradoń Leon
Published: March 2023

Halloysite particles, with their unique multilayer nanostructure, are demonstrated here as highly efficient and readily available sorbent of heavy metals that can be easily scaled up and used in large-scale water remediation facilities. The various methods of raw material purification were applied, and their effects were verified using techniques such as BET isotherm (determination of specific surface area and size of pores), XRF analysis (composition), and SEM imaging (determination of morphology). A series of adsorption experiments for aqueous solutions of metal ions (i.e., lead, cadmium) were carried out to quantify the sorption capacity of halloysite particles for selected heavy metals. The ability of adequately activated halloysite to efficiently remove heavy metal ions from water solutions was confirmed. The value of the zeta potential of raw and purified halloysite particles in water was determined. This enables us to understand its importance for the sorption of positively charged ions (metal, organics) at various pH values. The adsorption process conducted in the pH range of 6.0–6.5 showed significant improvement compared to the acidic conditions (pH value 3.0–3.5) and resulted in a high sorption capacity of lead ions—above 24.3 mg/g for the sulphuric acid-treated sample. The atomic scale ab initio calculations revealed a significant difference in adsorption energy between the external siloxane surface and cross-sectional interlayer surface, resulting in pronounced adsorption anisotropy. A low energy barrier was calculated for the interlayer migration of heavy metals into the halloysite interior, facilitating access to the active sites in these regions, thus significantly increasing the sorption capacity and kinetics. DFT (density functional theory) calculations supporting this study allowed for predicting the sorption potential of pure halloysite structure towards heavy metals. To confront it with experimental results, it was crucial to determine proper purification conditions to obtain such a developed structure from the mineral ore. The results show a massive increase in the BET area and confirm a high sorption potential of modified halloysite towards heavy metals.
Time-Restricted Feeding Modifies the Fecal Lipidome and the Gut Microbiota
Authors: Rust Bret M. , Picklo Matthew J. , Yan Lin , Mehus Aaron A. , Zeng Huawei
Published: March 2023

Time-restricted feeding (TRF) has been identified as an approach to reduce the risk of obesity-related metabolic diseases. We hypothesize that TRF triggers a change in nutrient (e.g., dietary fat) absorption due to shortened feeding times, which subsequently alters the fecal microbiome and lipidome. In this report, three groups of C57BL/6 mice were fed either a control diet with ad libitum feeding (16% energy from fat) (CTRL-AL), a high-fat diet (48% energy from fat) with ad libitum feeding (HF-AL), or a high-fat diet with time-restricted feeding (HF-TRF) for 12 weeks. No changes in microbiota at the phylum level were detected, but eight taxonomic families were altered by either feeding timing or dietary fat content. The HF-AL diet doubled the total fecal fatty acid content of the CTRL-AL diet, while the HF-TRF doubled the total fecal fatty acid content of the HF-AL diet. Primary fecal bile acids were unaffected by diet. Total short-chain fatty acids were reduced by HF-AL, but this effect was diminished by HF-TRF. Each diet produced distinct relationships between the relative abundance of taxa and fecal lipids. The anti-obesogenic effects of TRF in HF diets are partly due to the increase in fat excretion in the feces. Furthermore, fat content and feeding timing differentially affect the fecal microbiota and the relationship between the microbiota and fecal lipids.
Association of Circulating Branched-Chain Amino Acids with Cardiovascular Diseases: A Mendelian Randomization Study
Authors: Xu Huan , Wang Xuanyang , Geng Guannan , Xu Xiaoqing , Liu Lin , Zhang Yuntao , Wang Ziqi , Wang Lulu , Li Ying
Published: March 2023

Background: There have been reports linking branched-chain amino acids (BCAAs) to the hazard of various cardiovascular diseases (CVDs); however, the causal role of this relationship is still unclear. We conducted a study using bi-directional two-sample Mendelian randomization (MR) with the aim of investigating the possible causal correlation between BCAAs and 13 types of cardiovascular diseases. Methods: The study analyzed data of the largest genome-wide association studies (GWAS) published for the total BCAAs, encompassing isoleucine, leucine, and valine, which were obtained from the UK Biobank, as well as data for 13 cardiovascular endpoints from the MRC-IEU, the FinnGen consortium, and the EBI database. The approach of the primary dissection used became the inverse-variance-weighted (IVW) approach, with additional analyses using the MR-PRESSO global test as well as MR-Egger regression with a view to determining horizontal pleiotropy. Heterogeneity was evaluated by means of Cochran’s Q test. The study also conducted logistic regression dissection for the sake of investigating the correlation between cardiovascular events and serum BCAAs in the UK biobank cohort study. Results: In this study, it was found that individuals with a genetic predisposition to more elevated levels for circulating total BCAAs had a higher hazard of peripheral arterial disease (OR 1.400, 95% CI 1.063, 1.844; p = 0.017) in addition to stroke (OR 1.266, 95% CI 1.012, 1.585; p = 0.039); circulating valine casually increased the risk of intracerebral hemorrhage (OR 1.760, 95% CI 1.116, 2.776; p = 0.015), along with stroke (OR 1.269, 95% CI 1.079, 1.492; p = 0.004); genetically predicted isoleucine showed a positive association with peripheral arterial disease (OR 1.466, 95% CI 1.044, 2.058; p = 0.027), along with cardioembolic stroke (OR 1.547, 95% CI 1.126, 2.124; p = 0.007); furthermore, leucine causally associated with stroke (OR 1.310, 95% CI 1.031, 1.663, p = 0.027). In the UK Biobank cohort study, we detected that total BCAAs (OR: 1.285; 95% CI: 1.009, 1.636), valine (OR: 1.287; 95% CI: 1.009, 1.642), and isoleucine (OR: 1.352; 95% CI: 1.064, 1.718) were independently linked to stroke, but not leucine (OR: 1.146; 95% CI: 0.901, 1.458). No such association was found for BCAAs with peripheral arterial disease and intracerebral hemorrhage in the cohort study. Conclusions: In summary, circulating total BCAAs and valine may be causally associated with stroke. The association of BCAAs with other CVD events needs further study.
Consumption of Solnul™ Resistant Potato Starch Produces a Prebiotic Effect in a Randomized, Placebo-Controlled Clinical Trial
Authors: Bush Jason R. , Baisley Joshua , Harding Scott V. , Alfa Michelle J.
Published: March 2023

The effects of resistant starch at high doses have been well-characterized, but the potential prebiotic effects of resistant starch at doses comparable to oligosaccharide prebiotics have not been evaluated. A three-arm randomized, double-blind, placebo-controlled clinical trial was conducted to evaluate the effect of 3.5 g and 7 g daily doses of Solnul™ resistant potato starch (RPS) on beneficial populations of gut bacteria and stool consistency after a 4-week period. The relative abundance of Bifidobacterium and Akkermansia was determined by employing 16Sv4 sequencing of stool samples. To assess the effect of RPS on laxation and bowel movements, stools were recorded and scored using the Bristol Stool Form Scale. Participants consuming 3.5 g/day of RPS experienced significantly greater changes in Bifidobacterium and Akkermansia compared to the placebo after 4 weeks. The number of diarrhea- and constipation-associated bowel movements were both significantly lower in the 3.5 g RPS arm compared to the placebo group. Participants consuming 7 g of RPS responded similarly to those in the 3.5 g arm. Our analyses demonstrate that Solnul™ RPS has a prebiotic effect when consumed for 4 weeks at the 3.5 g per day dose, stimulating increases in beneficial health-associated bacteria and reducing diarrhea- and constipation-associated bowel movements when compared to the placebo group.
Transition of Orbital Electrons by Electromagnetic Waves
Authors: Muhibbullah Muhammad , Ikuma Yasuro
Published: March 2023

An electromagnetic (EM) wave is a form of continuous energy, of which both the frequency and the amplitude are parts, as shown in a recent report. All the facts relating to the photoelectric effect are explained by the new modified EM wave concept. Since the photon concept is not able to explain the intensity effect and the ejection direction clearly, it cannot be used to explain nonlinear optical phenomena clearly. The current understanding of the interaction process between orbital electrons and light may not be realistic. In this work, the electron transition process is explained with the new modified EM wave concept. The orbital electrons of a material rotate circularly by the sinusoidal fields of the EM waves. In this way, the electrons absorb light energy as rotational kinetic energy. During the first rotational cycle, the electrons with large enough radii face different potential barriers in neighboring orbits. Consequently, the electrons’ speed is obstructed, and the electrons move behind their natural places (phase); in other words, the electrons cannot follow the required phase of EM waves. Thus, sufficient energetic electrons are scattered from their orbit. The high-intensity EM waves reach the inner orbits of the targeted atom and transit electrons from different orbits. The light can regenerate through processes with different frequencies. The frequency of the regenerated light can be higher than that of primary light, depending on the energy (frequency and amplitude) of the primary light. The results of previous reports match the prediction of the new concept of EM waves. The new wave concept may be able to explain all photonic behaviors of light clearly.
Acetabular Wall Weakening in Total Hip Arthroplasty: A Pilot Study
Authors: Gautreaux Madeline , Kautz Steven , Martin Zashiana , Morgan Edward , Barton R. Shane , Dubose Matthew , McBride Hayden , Solitro Giovanni F.
Published: March 2023

Total hip arthroplasty is a widely performed operation allowing disabled patients to improve their quality of life to a degree greater than any other elective procedure. Planning for a THA requires adequate patient assessment and preoperative characterizations of acetabular bone loss via radiographs and specific classification schemes. Some surgeons may be inclined to ream at a larger diameter thinking it would lead to a more stable press-fit, but this could be detrimental to the acetabular wall, leading to intraoperative fracture. In the attempt to reduce the incidence of intraoperative fractures, the current study aims to identify how increased reaming diameter degrades and weakens the acetabular rim strength. We hypothesized that there is proportionality between the reaming diameter and the reduction in acetabular strength. To test this hypothesis, this study used bone surrogates, templated from CT scans, and reamed at different diameters. The obtained bone surrogate models were then tested using an Intron 8874 mechanical testing machine (Instron, Norwood, MA) equipped with a custom-made fixture. Analysis of variance (ANOVA) was used to identify differences among reamed diameters while linear regression was used to identify the relationship between reamed diameters and acetabular strength. We found a moderate correlation between increasing reaming diameter that induced thinning of the acetabular wall and radial load damage. For the simplified acetabular model used in this study, it supported our hypothesis and is a promising first attempt in providing quantitative data for acetabular weakening induced by reaming.
Role of Non-Covalent Interactions in Carbonic Anhydrase I—Topiramate Complex Based on QM/MM Approach
Authors: Wojtkowiak Kamil , Jezierska Aneta
Published: March 2023

Carbonic anhydrase (CA) I with a Topiramate (TPM) complex was investigated on the basis of a Quantum Mechanics/Molecular Mechanics (QM/MM) approach. The QM part was treated using Density Functional Theory (DFT) while the MM was simulated using Amberff14SB and GAFF force fields. In addition, the TIP3P model was applied to reproduce the polar environment influence on the studied complex. Next, three snapshots (after 5 ps, 10 ps, and 15 ps of the simulation time) were taken from the obtained trajectory to provide an insight into the non-covalent interactions present between the ligand and binding pocket of the protein. Our special attention was devoted to the binding site rearrangement, which is known in the literature concerning the complex. This part of the computations was performed using ωB97X functional with Grimme D3 dispersion corrections as well as a Becke–Johnson damping function (D3-BJ). Two basis sets were applied: def2-SVP (for larger models) and def2-TZVPD (for smaller models), respectively. In order to detect and describe non-covalent interactions between amino acids of the binding pocket and the ligand, Independent Gradient Model based on Hirshfeld partitioning (IGMH), Interaction Region Indicator (IRI), Quantum Theory of Atoms in Molecules (QTAIM) and Natural Bond Orbitals (NBO) methods were employed. Finally, Symmetry-Adapted Perturbation Theory (SAPT) was applied for energy decomposition between the ligand and protein. It was found that during the simulation time, the ligand position in the binding site was preserved. Nonetheless, amino acids interacting with TPM were exchanging during the simulation, thus showing the binding site reorganization. The energy partitioning revealed that dispersion and electrostatics are decisive factors that are responsible for the complex stability.
Recent Advances in Anti-Tuberculosis Drug Discovery Based on Hydrazide–Hydrazone and Thiadiazole Derivatives Targeting InhA
Authors: Teneva Yoanna , Simeonova Rumyana , Valcheva Violeta , Angelova Violina T.
Published: March 2023

Tuberculosis is an extremely serious problem of global public health. Its incidence is worsened by the presence of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis. More serious forms of drug resistance have been observed in recent years. Therefore, the discovery and/or synthesis of new potent and less toxic anti-tubercular compounds is very critical, especially having in mind the consequences and the delays in treatment caused by the COVID-19 pandemic. Enoyl-acyl carrier protein reductase (InhA) is an important enzyme involved in the biosynthesis of mycolic acid, a major component of the M. tuberculosis cell wall. At the same time, it is a key enzyme in the development of drug resistance, making it an important target for the discovery of new antimycobacterial agents. Many different chemical scaffolds, including hydrazide hydrazones and thiadiazoles, have been evaluated for their InhA inhibitory activity. The aim of this review is to evaluate recently described hydrazide-hydrazone- and thiadiazole-containing derivatives that inhibit InhA activity, resulting in antimycobacterial effects. In addition, a brief review of the mechanisms of action of currently available anti-tuberculosis drugs is provided, including recently approved agents and molecules in clinical trials.