bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Objective Scheduling Strategy of Mine Transportation Robot Based on Three-Dimensional Loading Constraint
Authors: Yan Xuanxuan , Wang Guorong , Jiang Kuosheng , Kou Ziming , Wang Kaisong , Zhang Lixiang
Published: March 2023

In an attempt to solve the problems of the low intelligent distribution degree and high working intensity of auxiliary transportation systems in underground coal mines, an intelligent distribution strategy of materials in the whole mine is put forward. Firstly, combined with the characteristics of materials and standard containers, a three-dimensional loading model is established with the goal of maximizing the space utilization of standard containers, and a three-dimensional space segmentation heuristic algorithm is used to solve the material loading scheme. Then, the multi-objective optimization model of distribution parameters is established with the goal of the shortest delivery distance, the shortest delay time, and the fewest number of delivery vehicles, and the dual-layer genetic algorithm is used to solve the distribution scheme. Finally, the spatiotemporal conversion coefficient is designed to solve the task list by hierarchical clustering, and the solution time is reduced by 30%. The results show that the dual-layer genetic algorithm based on hierarchical clustering has good adaptability in complex material scheduling scenarios.
Triassic Appinite from the Qinling Orogen (Central China): Hydrous Melting of Depleted Mantle Wedge in Post-Collision Stage
Authors: Liu Hang , Gong Hujun , Luo Fenhong , Zhang Yaqin , Dang Ben
Published: March 2023

Mantle wedge melting and the formation of hydrous mafic melts in collision orogenic belts have great significance for crustal melting and the related granitic magmatism, which can provide key messages about the crustal–mantle interaction in the convergent margin. This paper reported Late-Triassic appinite (212 ± 2 Ma), which is closely associated with Late-Triassic granites. The large euhedral hornblende crystals in the appinite indicate a water-rich feature. This appinite displays low SiO2 (46.55% to 50.44%) contents, high MgO (7.62 to 10.76%) and Cr and Ni contents, with high Mg# values of 61 to 75. It also displays insignificant Eu anomalies (Eu*/Eu = 0.91 to 0.93), high Sr (596 to 676 ppm) contents and moderate Sr/Y (34 to 40) ratios; these features are similar to those of Sanukite formed by the hydrous melting of the mantle wedge. Its depleted zircon Lu-Hf isotopic composition (εHf(t) = +0.97 to +18.21) indicates a depleted mantle source. Zircons in the appinite display extremely high Ti-in zircon temperatures (>1000 °C) and high oxygen fugacity, indicating a high-temperature hydrous condition. In combination with its typical arc-like trace element geochemistry (depletion in Nb, Ta and Ti), it is proposed that this appinite represents hydrous mafic melts that derived from the melting of the depleted sub-arc mantle wedge. The occurrence of this appinite has great significance for the further understanding of Triassic granitic magmatism and potential magmatic metal ore deposits in the Qinling orogenic belt.
An Investigation of a Natural Biosorbent for Removing Methylene Blue Dye from Aqueous Solution
Authors: Alhogbi Basma G. , Al Balawi Ghadeer S.
Published: March 2023

T he current study reports the use of zeolite prepared from a kaolin composite via physical mixing with different ratios from fiber of palm tree (Zeo-FPT) as a sustainable solid sorbent for the removal of methylene blue (MB) dye from aqueous solutions. The prepared biosorbent was fully characterized using XRD, TGA, SEM, and FTIR. The impacts of various analytical parameters, for example, contact time, dosage, MB dye concentration, and the pH of the solution, on the dye adsorption process were determined. After a contact time of 40 min, the capacity to remove MB dye was 0.438 mg g−1 at a Zeo-FPT composition ratio of 1F:1Z. At pH 8, Zeo-FPT (1F:1Z) had a removal efficiency of 87% at a sorbent dosage of 0.5 g for a concentration of MB dye in an aqueous phase of 10 mg L−1. The experimental data were also analyzed using the kinetic and adsorption isotherm models. The retention process fitted well with the pseudo-second-order model (R2 0.998), where the Qe,calc of 0.353 mg g−1 was in acceptable agreement with the Qe,exp of 0.438 mg g−1. The data also fitted well with the Freundlich isotherm model, as indicated by the correlation coefficient value (R2 0.969). The Zeo-FPT attained a high percentage (99%) in the removal of MB dye from environmental water samples (tap water, bottled water, and well water). Thus, it can be concluded that the proposed zeolite composite with fiber of palm tree (Zeo-FPT) is a suitable, environmentally friendly, and low-cost adsorbent for removing dyes from wastewater.
Defect-Enriched Graphene Nanoribbons Tune the Adsorption Behavior of the Mediator to Boost the Lactate/Oxygen Biofuel Cell
Authors: Feng Xiaoyu , Ning Yongyue , Wu Zhongdong , Li Zihan , Xu Cuixing , Li Gangyong , Hu Zongqian
Published: March 2023

Owing to the high efficiency and specificity in moderate conditions, enzymatic biofuel cells (EBFCs) have gained significant interest as a promising energy source for wearable devices. However, the instability of the bioelectrode and the lack of efficient electrical communication between the enzymes and electrodes are the main obstacles. Herein, defect-enriched 3D graphene nanoribbons (GNRs) frameworks are fabricated by unzipping multiwall carbon nanotubes, followed by thermal annealing. It is found that defective carbon shows stronger adsorption energy towards the polar mediators than the pristine carbon, which is beneficial to improving the stability of the bioelectrodes. Consequently, the EBFCs equipped with the GNRs exhibit a significantly enhanced bioelectrocatalytic performance and operational stability, delivering an open-circuit voltage and power density of 0.62 V, 70.7 μW/cm2, and 0.58 V, 18.6 μW/cm2 in phosphate buffer solution and artificial tear, respectively, which represent the high levels among the reported literature. This work provides a design principle according to which defective carbon materials could be more suitable for the immobilization of biocatalytic components in the application of EBFCs.
Microwave Synthesis of Visible-Light-Activated g-C3N4/TiO2 Photocatalysts
Authors: Matias Maria Leonor , Reis-Machado Ana S. , Rodrigues Joana , Calmeiro Tomás , Deuermeier Jonas , Pimentel Ana , Fortunato Elvira , Martins Rodrigo , Nunes Daniela
Published: March 2023

The preparation of visible-light-driven photocatalysts has become highly appealing for environmental remediation through simple, fast and green chemical methods. The current study reports the synthesis and characterization of graphitic carbon nitride/titanium dioxide (g-C3N4/TiO2) heterostructures through a fast (1 h) and simple microwave-assisted approach. Different g-C3N4 amounts mixed with TiO2 (15, 30 and 45 wt. %) were investigated for the photocatalytic degradation of a recalcitrant azo dye (methyl orange (MO)) under solar simulating light. X-ray diffraction (XRD) revealed the anatase TiO2 phase for the pure material and all heterostructures produced. Scanning electron microscopy (SEM) showed that by increasing the amount of g-C3N4 in the synthesis, large TiO2 aggregates composed of irregularly shaped particles were disintegrated and resulted in smaller ones, composing a film that covered the g-C3N4 nanosheets. Scanning transmission electron microscopy (STEM) analyses confirmed the existence of an effective interface between a g-C3N4 nanosheet and a TiO2 nanocrystal. X-ray photoelectron spectroscopy (XPS) evidenced no chemical alterations to both g-C3N4 and TiO2 at the heterostructure. The visible-light absorption shift was indicated by the red shift in the absorption onset through the ultraviolet-visible (UV-VIS) absorption spectra. The 30 wt. % of g-C3N4/TiO2 heterostructure showed the best photocatalytic performance, with a MO dye degradation of 85% in 4 h, corresponding to an enhanced efficiency of almost 2 and 10 times greater than that of pure TiO2 and g-C3N4 nanosheets, respectively. Superoxide radical species were found to be the most active radical species in the MO photodegradation process. The creation of a type-II heterostructure is highly suggested due to the negligible participation of hydroxyl radical species in the photodegradation process. The superior photocatalytic activity was attributed to the synergy of g-C3N4 and TiO2 materials.
Synthesized Zeolite Based on Egyptian Boiler Ash Residue and Kaolin for the Effective Removal of Heavy Metal Ions from Industrial Wastewater
Authors: Ibrahim Ahmed H. , Lyu Xianjun , ElDeeb Amr B.
Published: March 2023

The increase of global environmental restrictions concerning solid and liquid industrial waste, in addition to the problem of climate change, which leads to a shortage of clean water resources, has raised interest in developing alternative and eco-friendly technologies for recycling and reducing the amount of these wastes. This study aims to utilize Sulfuric acid solid residue (SASR), which is produced as a useless waste in the multi-processing of Egyptian boiler ash. A modified mixture of SASR and kaolin was used as the basic component for synthesizing cost-effective zeolite using the alkaline fusion-hydrothermal method for the removal of heavy metal ions from industrial wastewater. The factors affecting the synthesis of zeolite, including the fusion temperature and SASR: kaolin mixing ratios, were investigated. The synthesized zeolite was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), particle size analysis (PSD) and N2 adsorption-desorption. The SASR: kaolin weight ratio of 1:1.5 yields faujasite and sodalite zeolite with 85.21% crystallinity, which then shows the best composition and characteristics of the synthesized zeolite. The factors affecting the adsorption of Zn2+, Pb2+, Cu2+, and Cd2+ ions from wastewater on synthesized zeolite surfaces, including the effect of pH, adsorbent dosage, contact time, initial concentration, and temperature, have been investigated. The obtained results indicate that a pseudo-second-order kinetic model and Langmuir isotherm model describe the adsorption process. The maximum adsorption capacities of Zn2+, Pb2+, Cu2+, and Cd2+ ions onto zeolite at 20 °C were 12.025, 15.96, 12.247, and 16.17 mg·g−1, respectively. The main mechanisms controlling the removal of these metal ions from aqueous solution by synthesized zeolite were proposed to be either surface adsorption, precipitation, or ion exchange. The quality of the wastewater sample obtained from the Egyptian General Petroleum Corporation (Eastern Desert, Egypt) was highly improved using the synthesized zeolite and the content of heavy metal ions was significantly reduced, which enhances the utilization of the treated water in agriculture.
Influence of Bulk Nanobubbles Generated by Acoustic Cavitation on Powder Microstructure and Rehydration Characteristics of Spray-Dried Milk Protein Concentrate Powders
Authors: Babu Karthik Sajith , Amamcharla Jayendra K.
Published: March 2023

Bulk nanobubbles (BNBs) have widespread applications in various fields of science due to numerous peculiar characteristics. Despite significant applications, only limited investigations are available on the application of BNBs in food processing. In the present study, a continuous acoustic cavitation technique was used to generate bulk nanobubbles (BNBs). The aim of this study was to evaluate the influence of BNB incorporation on the processability and spray drying of milk protein concentrate (MPC) dispersions. MPC powders were reconstituted to the desired total solids and incorporated with BNBs using acoustic cavitation as per the experimental design. The control MPC (C-MPC) and BNB-incorporated MPC (BNB-MPC) dispersions were analyzed for rheological, functional, and microstructural properties. The viscosity significantly decreased (p < 0.05) at all the amplitudes studied. The microscopic observations of BNB-MPC dispersions showed less aggregated microstructures and greater structural differences compared with C-MPC dispersions, therefore lowering the viscosity. The viscosity of BNB incorporated (90% amplitude) MPC dispersions at 19% total solids at a shear rate of 100 s−1 significantly decreased to 15.43 mPa·s (C-MPC: 201 mPa·s), a net decrease in viscosity by ~90% with the BNB treatment. The control and BNB incorporated MPC dispersions were spray-dried, and the resultant powders were characterized in terms of powder microstructure and rehydration characteristics. Focused beam reflectance measurement of the BNB-MPC powders indicated higher counts of fine particles (<10 μm) during dissolution, signifying that BNB-MPC powders exhibited better rehydration properties than the C-MPC powders. The enhanced powder rehydration with the BNB incorporation was attributed to the powder microstructure. Overall, reducing the viscosity of feed by BNB incorporation can enhance the performance of the evaporator. This study, therefore, recommends the possibility of using BNB treatment for more efficient drying while improving the functional properties of the resultant MPC powders.
The Effect of Silica Particle Size on the Mechanical Enhancement of Polymer Nanocomposites
Authors: Kontou Evagelia , Christopoulos Angelos , Koralli Panagiota , Mouzakis Dionysios E.
Published: March 2023

In the present work, SiO2micro/nanocomposites based on poly-lactic acid (PLA) and an epoxy resin were prepared and experimentally studied. The silica particles were of varying sizes from the nano to micro scale at the same loading. The mechanical and thermomechanical performance, in terms of dynamic mechanical analysis, of the composites prepared was studied in combination with scanning electron microscopy (SEM). Finite element analysis (FEA) has been performed to analyze the Young’s modulus of the composites. A comparison with the results of a well-known analytical model, taking into account the filler’s size and the presence of interphase, was also performed. The general trend is that the reinforcement is higher for the nanosized particles, but it is important to conduct supplementary studies on the combined effect of the matrix type, the size of the nanoparticles, and the dispersion quality. A significant mechanical enhancement was obtained, particularly in the Resin/based nanocomposites.
One Pot Self-Assembling Fe@PANI Core–Shell Nanowires for Radar Absorption Application
Authors: Lin Chung-Kwei , Chiou Yuh-Jing , Tsou Sheng-Jung , Chung Chih-Yi , Chao Chen-Chun , Yang Ruey-Bing
Published: March 2023

The one-pot process, which combines the polymerization of polyaniline (i.e., PANI) with subsequent reduction of iron nanowire (i.e., Fe NW) under a magnetic field, was developed to produce Fe@PANI core–shell nanowires. The synthesized nanowires with various PANI additions (0–30 wt.%) were characterized and used as microwave absorbers. Epoxy composites with 10 wt.% absorbers were prepared and examined using the coaxial method to reveal their microwave absorbing performance. Experimental results showed that the Fe NWs with PANI additions (0–30 wt.%) had average diameters ranging from 124.72 to 309.73 nm. As PANI addition increases, the α-Fe phase content and the grain size decrease, while the specific surface area increases. The nanowire-added composites exhibited superior microwave absorption performance with wide effective absorption bandwidths. Among them, Fe@PANI-90/10 exhibits the best overall microwave absorption performance. With a thickness of 2.3 mm, effective absorption bandwidth was the widest and reached 3.73 GHz, ranging from 9.73 to 13.46 GHz. Whereas with a thickness of 5.4 mm, Fe@PANI-90/10 reached the best reflection loss of −31.87 dB at 4.53 GHz.
Multifunctional Layered Double Hydroxides for Drug Delivery and Imaging
Authors: Yu Seungjin , Choi Goeun , Choy Jin-Ho
Published: March 2023

Two-dimensional nanomaterials, particularly layered double hydroxides (LDHs), have been widely applied in the biomedical field owing to their biocompatibility, biodegradability, controllable drug release/loading ability, and enhanced cellular permeability. Since the first study analyzing intercalative LDHs in 1999, numerous studies have investigated their biomedical applications, including drug delivery and imaging; recent research has focused on the design and development of multifunctional LDHs. This review summarizes the synthetic strategies and in-vivo and in-vitro therapeutic actions and targeting properties of single-function LDH-based nanohybrids and recently reported (from 2019 to 2023) multifunctional systems developed for drug delivery and/or bio-imaging.
Enhanced Electroluminescence from a Silicon Nanocrystal/Silicon Carbide Multilayer Light-Emitting Diode
Authors: Sun Teng , Li Dongke , Chen Jiaming , Wang Yuhao , Han Junnan , Zhu Ting , Li Wei , Xu Jun , Chen Kunji
Published: March 2023

Developing high-performance Si-based light-emitting devices is the key step to realizing all-Si-based optical telecommunication. Usually, silica (SiO2) as the host matrix is used to passivate silicon nanocrystals, and a strong quantum confinement effect can be observed due to the large band offset between Si and SiO2 (~8.9 eV). Here, for further development of device properties, we fabricate Si nanocrystals (NCs)/SiC multilayers and study the changes in photoelectric properties of the LEDs induced by P dopants. PL peaks centered at 500 nm, 650 nm and 800 nm can be detected, which are attributed to surface states between SiC and Si NCs, amorphous SiC and Si NCs, respectively. PL intensities are first enhanced and then decreased after introducing P dopants. It is believed that the enhancement is due to passivation of the Si dangling bonds at the surface of Si NCs, while the suppression is ascribed to enhanced Auger recombination and new defects induced by excessive P dopants. Un-doped and P-doped LEDs based on Si NCs/SiC multilayers are fabricated and the performance is enhanced greatly after doping. As fitted, emission peaks near 500 nm and 750 nm can be detected. The current density-voltage properties indicate that the carrier transport process is dominated by FN tunneling mechanisms, while the linear relationship between the integrated EL intensity and injection current illustrates that the EL mechanism is attributed to recombination of electron–hole pairs at Si NCs induced by bipolar injection. After doping, the integrated EL intensities are enhanced by about an order of magnitude, indicating that EQE is greatly improved.
The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
Authors: Hajji Najla , Russo Ilaria , Bianco Jessica , Piazza Ornella , Iovino Paola , Santonicola Antonella , Ciacci Carolina
Published: March 2023

The existing literature indicates that Globularia alypum L. (GA) influences inflammation and oxidative stress modulation in rats and in vitro. The present study aims to investigate the effects of this plant in patients with ulcerative colitis (UC) and normal controls. In our experiments, we pretreated colon biopsies from 46 UC patients and normal controls with GA leaves aqueous extract (GAAE) used at two concentrations (50 and 100 µg/mL) for 3 h, followed by Lipopolysaccharides (from Escherichia coli) stimulation. We analyzed the effects on inflammation by studying the cyclo-oxygenase-2, the intercellular adhesion molecule-1, the nuclear factor kappa B, and p38 mitogen-activated protein kinase expression. Moreover, we assessed the levels of interleukin 6, the superoxide dismutase activity, and nitric oxide release in the supernatant of cultures. Our data showed that GAAE influences UC patients and normal controls for most studied markers and enzymes. These results acknowledge, with some scientific evidence, the traditional belief in the anti-inflammatory properties of GA and represent the first demonstration of its effect in a human in vitro model of inflammatory conditions.
Effects of Astaxanthin, Lutein, and Zeaxanthin on Eye–Hand Coordination and Smooth-Pursuit Eye Movement after Visual Display Terminal Operation in Healthy Subjects: A Randomized, Double-Blind Placebo-Controlled Intergroup Trial
Authors: Yoshida Keisuke , Sakai Osamu , Honda Tomoo , Kikuya Tomio , Takeda Ryuji , Sawabe Akiyoshi , Inaba Masamaru , Koike Chieko
Published: March 2023

(1) Background: The impairment of eye–hand coordination and smooth-pursuit eye movement caused by visual display terminal (VDT) operation is thought to impair daily living activities, for which no effective methods are currently known. On the other hand, various food ingredients, including astaxanthin, lutein, and zeaxanthin, are known to help improve the eye health of VDT operators. This study aimed to test the hypothesis that the combination of astaxanthin, lutein, and zeaxanthin can prevent the impairment of eye–hand coordination and smooth-pursuit eye movement caused by VDT operation. (2) Methods: We conducted a randomized, placebo-controlled, parallel-group clinical trial. Healthy subjects who regularly worked with VDTs were randomly assigned to the active and placebo groups. All of the subjects took soft capsules containing 6 mg of astaxanthin, 10 mg of lutein, and 2 mg of zeaxanthin or placebo soft capsules once daily for eight weeks. We evaluated the eye–hand coordination, smooth-pursuit eye movements, and macular pigment optical density (MPOD) at 0, two, four, and eight weeks after soft-capsule intake. (3) Results: The active group showed significantly improved eye–hand coordination after VDT operation at eight weeks. However, there was no clear improvement in the effect of the supplementation on smooth-pursuit eye movements. The active group also showed a significant increase in MPOD levels. (4) Conclusions: Consumption of a supplement containing astaxanthin, lutein, and zeaxanthin mitigates the decline of eye–hand coordination after VDT operation.
Impact of Dairy Products and Plant-Based Alternatives on Dental Health: Food Matrix Effects
Authors: Shkembi Blerina , Huppertz Thom
Published: March 2023

The impact of dairy products on dental health has been researched widely and shows an important role of various constituents, as well as the specific product matrix, in maintaining and improving dental health. These include, for instance, the position of lactose as the least cariogenic fermentable sugar, the high levels of calcium and phosphate, the presence of phosphopeptides as well as the antibacterial peptides lactoferrin and lysozyme and high buffering capacity. With plant-based alternatives for dairy products being developed and marketed these days, the specific benefits of dairy products in relation to dental health are often overlooked and most products contain more cariogenic carbohydrates, lack phosphopeptides, and have fewer minerals and less buffering capacity. Comparative studies performed to date indeed suggest that plant-based products do not match dairy counterparts when it comes to maintaining and improving dental health. Careful consideration of these aspects is required in relation to future developments of products and human diets. In this paper, we review the impact of dairy products and plant-based dairy alternatives on dental health.
Different Dietary Approaches, Non-Alcoholic Fatty Liver Disease and Cardiovascular Disease: A Literature Review
Authors: Torres-Peña Jose D. , Arenas-de Larriva Antonio P. , Alcala-Diaz Juan F. , Lopez-Miranda Jose , Delgado-Lista Javier
Published: March 2023

Non-alcoholic fatty liver disease (NAFLD) is the first cause of chronic liver disease and is also associated with other harmful entities such as obesity, metabolic syndrome, dyslipidemia, and diabetes. NAFLD is a significant public health concern worldwide, impacting individuals of all ages, and its prevalence is projected to increase in the near future due to its connection with obesity. Intrinsic (genetics) and external (lifestyle) factors may also modulate NAFLD, and, in turn, may partly explain the observed relationship between NAFLD and cardiovascular disease (CVD). Although many drugs are been tested to treat NAFLD, to date, no drug has indication to specifically treat this disorder. Thus, the current management of NAFLD relies on lifestyle modifications and specifically on weight loss, physical activity, and the intake of a healthy diet. In the present narrative review, we will discuss the effects of certain dietary patterns on NAFLD incidence and progression.
Dietary Patterns of Off-Reserve Indigenous Peoples in Canada and Their Association with Chronic Conditions
Authors: Keshavarz Pardis , Lane Ginny , Pahwa Punam , Lieffers Jessica , Shafiee Mojtaba , Finkas Kelly , Desmarais Marisa , Vatanparast Hassan
Published: March 2023

Nationally representative nutrition surveys (Canadian Community Health Survey (CCHS) Cycle 2.2, Nutrition 2004 and 2015) were used to examine dietary patterns and their association with socioeconomic/sociodemographic factors and chronic conditions in off-reserve Indigenous population in Canada. A cluster analysis was used to identify dietary patterns (DPs), and the Nutrient Rich Food Index (NRF 9.3) was used as the diet quality score and stratified by age/gender groups. In 2004 (n = 1528), the dominant DPs among Indigenous adults (age = 41 ± 2.3) were “Mixed” (mean NRF = 450 ± 12) and “Unhealthy” among men (mean NRF = 426 ± 18), “Fruits” among women (mean NRF = 526 ± 29), and “High-Fat/High-Sugar” among children (age = 10.2 ± 0.5) (mean NRF = 457 ± 12). In 2015 (n = 950), the dominant DPs were “Unhealthy” (mean NRF = 466 ± 6), “Mixed” (mean NRF = 485 ± 21), Healthy-Like (mean NRF = 568 ± 37), and “Mixed” (mean NRF = 510 ± 9) among adults (age = 45.6 ± 2.2), men, women, and children (age = 10.9 ± 0.3) respectively. The majority of Indigenous peoples had the “Unhealthy” DP with a low diet quality, which may contribute to a high prevalence of obesity and chronic diseases. The income level and smoking status among adults and physical inactivity among children were recognized as important factors that may be associated with the dietary intake of off-reserve Indigenous population.
Study on the New Role of Civil and Military Air Rescue Nurses in the Italian Context
Authors: Loi Francesca , Lucchetta Maria Raffaela , Mameli Claudio , Rosmarino Roberta , Oppes Giulio , Fuentes Ronald Jaimes , Avilez Gonzalez Ingrid Dallana , Aviles Gonzalez Cesar Ivan
Published: March 2023

Context: An emergency medical nurse is a health professional who operates at a very high level in the field of emergencies. The nurses of the critical area departments of the Territorial Emergency Department currently operate in the Sardinian helicopter rescue service. The effectiveness of the treatment that these nurses administer can be attributed to the quality of the previous and recurrent training that this unit must undergo. Objective: This study’s aim was to investigate the role of civil and military helicopter nurses in the context of Italian medical aid. Methods: A qualitative study, with a phenomenological approach, was conducted by interviewing 15 emergency medical nurses, using detailed recordings and transcripts. These findings were then compared to understand how nurses work outside their department of origin, how their training has influenced their ability to establish themselves outside it, and thus their ability to become part of a context considered to be of the highest level. Participants and research context: The personnel interviewed in this study were those who were working in the helibases of Cagliari, Olbia, and Alghero. The limitations of this study are linked to the impossibility of obtaining an internship at a company, because, at the time of the study, an agreement between the university and the Areus company was not active. Ethical considerations: Participation in this research was completely voluntary. In fact, the participants could cease participating at any time. Results: This study revealed issues related to training, preparation, motivation to carry out the role held, nursing autonomy, the willingness to collaborate between the various rescue organizations, the use of the helicopter rescue service, and possible improvements for this service. Conclusions: civil air rescue nurses can deepen their knowledge by examining the work of military air rescue nurses, because, although the operational contexts are different, some techniques used in a hostile environment are also applicable to civilian environments. By doing so, nurses could become independent team leaders for all intents and purposes, managing their own training, preparation, and technical skills.
Factors Influencing Depression in Adolescents Focusing on the Degree of Appearance Stress
Authors: Lim Mihye , Kwon Myoungjin
Published: March 2023

This descriptive cross-sectional study examined the factors that affect depression in adolescents focusing on how stressed they are regarding their appearance (hereafter, degree of appearance stress). Data from 6493 adolescents from the 2020 Korean Youth Risk Behavior Survey were used. Using SPSS 25.0, a complex sample plan file was created, weighted, and analyzed. The frequency, chi-square test, independent t-test, and linear regression were used for the complex sample analysis. The results showed that among adolescents with low appearance stress, depression was significantly affected by the number of breakfast meals, weight control efforts, smoking, loneliness, subjective physical appearance, and smartphone overdependence. Among those with high appearance stress, depression was significantly affected by academic grades, weight control efforts, drinking habits, loneliness, subjective physical appearance, and smartphone overdependence. Furthermore, these factors differed according to the degree of appearance stress. Thus, while developing interventions for mitigating depression in adolescents, the degree of appearance stress should be considered, and a differentiated strategy should be used accordingly.
How Do Nursing Students Perceive Moral Distress? An Interpretative Phenomenological Study
Authors: Gandossi Chiara , De Brasi Elvira Luana , Rosa Debora , Maffioli Sara , Zappa Sara , Villa Giulia , Manara Duilio Fiorenzo
Published: March 2023

Background: Research shows that the longer nurses care for terminally ill patients, the greater they experience moral distress. The same applies to nursing students. This study aims to analyze episodes of moral distress experienced by nursing students during end-of-life care of onco-hematologic patients in hospital settings. Methods: This study was conducted in the interpretative paradigm using a hermeneutic phenomenological approach and data were analyzed following the principles of the Interpretative Phenomenological Analysis. Results: Seventeen participants were included in the study. The research team identified eight themes: causes of moral distress; factors that worsen or influence the experience of moral distress; feelings and emotions in morally distressing events; morally distressing events and consultation; strategies to cope with moral distress; recovering from morally distressing events; end-of-life accompaniment; internship clinical training, and nursing curriculum. Conclusions: Moral distress is often related to poor communication or lack of communication between health care professionals and patients or relatives and to the inability to satisfy patients’ last needs and wants. Further studies are necessary to examine the quantitative dimension of moral distress in nursing students. Students frequently experience moral distress in the onco-hematological setting.
Relationship between Experience of Client Violence and Turnover Intention among Workers in Long-Term Care Facilities for Older Adults: Focusing on Nurses, Social Workers, and Care Workers
Authors: Choi Seunghoon , Shin Hyeri , Lee Minah , Han Kimyung
Published: March 2023

To prevent discontinuity of long-term care service and guarantee the quality of care, it is important to clarify the determinants of turnover intentions of long-term care workers. They are at a higher risk of experiencing violence—including physical, emotional, and sexual—from patients or their families, possibly leading to high turnover intention. This study aims to verify how having experienced client violence affect turnover intention of long-term care workers and to suggest implications to prevent frequent turnover in long-term care field. Logistic regression analysis was conducted between groups who have experienced client violence and who have not, using 2019 Korean LTC Survey data. Results revealed that, first, there were differences in determinants of turnover intention depending on groups. Second, having experienced client violence had a different effect on turnover intention based on personal characteristics. Third, gender and occupational differences were found. Based on our results, we highlighted the need for discussions on interventions to address client violence exposure among long-term care workers.
Syndrome of Transient Headache and Neurologic Deficits with Cerebrospinal Fluid Lymphocytosis (HaNDL): HHV-7 Finding in Cerebrospinal Fluid Challenges Diagnostic Criteria
Authors: Sundholm Anna , Gustafsson Rasmus , Karrenbauer Virginija
Published: March 2023

The syndrome of transient headache and neurologic deficits with cerebrospinal fluid lymphocytosis (HaNDL) is a rare, self-limiting condition with severe headaches combined with neurological symptoms. However, evidence-based recommendations on diagnostics and treatments are unavailable due to the condition’s rarity and unknown pathophysiology. A young man experiencing severe headache attacks fulfilled the HaNDL diagnostic criteria according to the third edition of the International Classification of Headache Disorders (ICHD-3). We present the dynamics of cerebrospinal fluid (CSF) biomarkers related to low human herpesvirus 7 (HHV-7) load and anti-inflammatory treatment outcomes. Low HHV-7 load may be an immunological trigger of HaNDL, such that elevated levels of CSF- chemokine (C-X-C motif) ligand 13 open a new way to interpret the role of B cells in HaNDL pathogenesis. We discuss the diagnostic challenge of HaNDL, according to the ICHD-3, in the case of pathogen presence at low load in CSF.
Migratory Wild Birds as Potential Long-Distance Transmitters of Toxoplasma gondii Infection
Authors: Dini Filippo Maria , Graziosi Giulia , Lupini Caterina , Catelli Elena , Galuppi Roberta
Published: March 2023

Toxoplasma gondii is a worldwide distributed zoonotic protozoan capable of infecting a wide range of mammals (including humans) and birds as intermediate hosts. Migratory wild birds, through interconnecting countries along their flyways, can play a role in the spatial spread of T. gondii and could contribute to its sylvatic cycle. Additionally, hunted wild birds used for meat consumption could represent a further source of human infection. To determine the presence of T. gondii in wild birds, a total of 50 individuals belonging to the Anseriformes and Charadriiformes orders were sampled during the 2021–2022 hunting season in Northern Italy. Cardiac muscle samples of three Northern shovelers (Anas clypeata), two wild mallards (A. platyrhynchos), one Eurasian teal (A. crecca), and one Northern lapwing (Vanellus vanellus) were positive for the molecular detection of T. gondii based on a targeted amplification of the B1 gene. A 14% (7/50) overall positivity was observed in the sampled population. Results from this study suggest a moderate exposure of wild aquatic birds to T. gondii, highlighting the importance of a further characterization of T. gondii in its wildlife hosts.
Purity, Danger, and Patriotism: The Struggle for a Veteran Home during the COVID-19 Pandemic
Authors: Kalofonos Ippolytos , McCoy Matthew
Published: March 2023

The coronavirus disease 2019 (COVID-19) pandemic rendered congregate shelter settings high risk, creating vulnerability for people experiencing homelessness (PEH). This study employed participant observation and interviews over 16 months in two Veteran encampments, one located on the grounds of the West Los Angeles Veteran Affairs Medical Center (WLAVA) serving as an emergency COVID-19 mitigation measure, and the other outside the WLAVA gates protesting the lack of onsite VA housing. Study participants included Veterans and VA personnel. Data were analyzed using grounded theory, accompanied by social theories of syndemics, purity, danger, and home. The study reveals that Veterans conceptualized home not merely as physical shelter but as encompassing a sense of inclusion and belonging. They sought a Veteran-run collective with a harm reduction approach to substance use, onsite healthcare, and inclusive terms (e.g., no sobriety requirements, curfews, mandatory treatment, or limited lengths of stay). The twin encampments created distinct forms of community and care that protected Veterans from COVID-19 infection and bolstered collective survival. The study concludes that PEH constitute and belong to communities that provide substantial benefits even while amplifying certain harms. Housing interventions must consider how unhoused individuals become, or fail to become, integrate into various communities, and foster therapeutic community connections.
Epidemiological Survey and Risk Factor Analysis of 14 Potential Pathogens in Golden Snub-Nosed Monkeys at Shennongjia National Nature Reserve, China
Authors: Qi Mingpu , Wang Qiankun , Wang Yu , Chen Yingyu , Hu Changmin , Yang Wanji , Wu Feng , Huang Tianpeng , Dawood Ali Sobhy , Zubair Muhammad , Li Xiang , Chen Jianguo , Robertson Ian Duncan , Chen Huanchun , Guo Aizhen
Published: March 2023

Golden snub-nosed monkeys (Rhinopithecus roxellanae) belong to Class A, the highest level of endangered primate species. Exploring the infection status of potential pathogens in golden snub-nosed monkeys is important for controlling associated diseases and protecting this species. The objective of this study was to investigate the seroprevalence for a number of potential pathogens and the prevalence of fecal adenovirus and rotavirus. A total of 283 fecal samples were collected from 100 golden snub-nosed monkeys in December 2014, June 2015, and January 2016; 26 blood samples were collected from 26 monkeys in June 2014, June 2015, January 2016 and November 2016 at Shennongjia National Reserve in Hubei, China. The infection of 11 potential viral diseases was examined serologically using an Indirect Enzyme-linked Immunosorbent Assay (iELISA) and Dot Immunobinding Assays (DIA), while the whole blood IFN-γ in vitro release assay was used to test tuberculosis (TB). In addition, fecal Adenovirus and Rotavirus were detected using Polymerase Chain Reaction (PCR). As a result, the Macacine herpesvirus-1 (MaHV-1), Golden snub-nosed monkey cytomegalovirus (GsmCMV), Simian foamy virus (SFV) and Hepatitis A virus (HAV) were detected with the seroprevalence of 57.7% (95% CI: 36.9, 76.6), 38.5% (95% CI: 20.2, 59.4), 26.9% (95% CI: 11.6, 47.8), and 7.7% (95% CI: 0.0, 84.2), respectively. Two fecal samples tested positive for Adenovirus (ADV) by PCR, with a prevalence of 0.7% (95% CI: 0.2, 2.5), and further, the amplification products were sequenced. Phylogenetic analysis revealed that they belonged to the HADV-G group. However, other pathogens, such as Coxsackievirus (CV), Measles virus (MeV), Rotavirus (RV), Simian immunodeficiency virus (SIV), Simian type D retroviruses (SRV), Simian-T-cell lymphotropic virus type 1 (STLV-1), Simian varicella virus (SVV), Simian virus 40 (SV40) and Mycobacterium tuberculosis complex (TB) were negative in all samples. In addition, a risk factor analysis indicated that the seroprevalence of MaHV-1 infection was significantly associated with old age (≥4 years). These results have important implications for understanding the health status and conservation of the endangered golden snub-nosed monkey population at Shennongjia Nature Reserve.
The Influence of Protein Charge and Molecular Weight on the Affinity of Aptamers
Authors: Drees Alissa , Trinh Tung Lam , Fischer Markus
Published: March 2023

Aptamers offer several advantages over antibodies. However, to ensure high affinity and specificity, a better understanding of the interactions between the nucleic-acid-based aptamers and their targets is mandatory. Therefore, we investigated the influence of two physical properties of proteins—molecular mass and charge—on the affinity of nucleic-acid-based aptamers. For this purpose, first, the affinity of two random oligonucleotides towards twelve proteins was determined. No binding was observed for proteins with a negative net charge towards the two oligonucleotides, while up to nanomolar affinity was determined for positively charged proteins with a high pI value. Second, a literature analysis comprising 369 aptamer–peptide/protein pairs was performed. The dataset included 296 different target peptides and proteins and is thus currently one of the largest databases for aptamers for proteins and peptides. The targets considered covered isoelectric points of 4.1–11.8 and a molecular weight range of 0.7–330 kDa, while the dissociation constants ranged from 50 fM to 29.5 µM. This also revealed a significant inverse correlation between the protein’s isoelectric point and the affinity of aptamers. In contrast, no trend was observed between the affinity and the molecular weight of the target protein with either approach.
Design, Synthesis, and Biological Evaluation of Sulfonamide Methoxypyridine Derivatives as Novel PI3K/mTOR Dual Inhibitors
Authors: Gao Haotian , Li Zaolin , Wang Kai , Zhang Yuhan , Wang Tong , Wang Fang , Xu Youjun
Published: March 2023

Phosphatidylinositol 3-kinase (PI3K) plays an important role in cell proliferation, survival, migration, and metabolism, and has become an effective target for cancer treatment. Meanwhile, inhibiting both PI3K and mammalian rapamycin receptor (mTOR) can simultaneously improve the efficiency of anti-tumor therapy. Herein, a series of 36 sulfonamide methoxypyridine derivatives with three different aromatic skeletons were synthesized as novel potent PI3K/mTOR dual inhibitors based on a scaffold hopping strategy. Enzyme inhibition assay and cell anti-proliferation assay were employed to assess all derivatives. Then, the effects of the most potent inhibitor on cell cycle and apoptosis were performed. Furthermore, the phosphorylation level of AKT, an important downstream effector of PI3K, was evaluated by Western blot assay. Finally, molecular docking was used to confirm the binding mode with PI3Kα and mTOR. Among them, 22c with the quinoline core showed strong PI3Kα kinase inhibitory activity (IC50 = 0.22 nM) and mTOR kinase inhibitory activity (IC50 = 23 nM). 22c also showed a strong proliferation inhibitory activity, both in MCF-7 cells (IC50 = 130 nM) and HCT-116 cells (IC50 = 20 nM). 22c could effectively cause cell cycle arrest in G0/G1 phase and induce apoptosis of HCT-116 cells. Western blot assay showed that 22c could decrease the phosphorylation of AKT at a low concentration. The results of the modeling docking study also confirmed the binding mode of 22c with PI3Kα and mTOR. Hence, 22c is a promising PI3K/mTOR dual inhibitor, which is worthy of further research in the area.
Polymeric Micellar Systems—A Special Emphasis on “Smart” Drug Delivery
Authors: Negut Irina , Bita Bogdan
Published: March 2023

Concurrent developments in anticancer nanotechnological treatments have been observed as the burden of cancer increases every year. The 21st century has seen a transformation in the study of medicine thanks to the advancement in the field of material science and nanomedicine. Improved drug delivery systems with proven efficacy and fewer side effects have been made possible. Nanoformulations with varied functions are being created using lipids, polymers, and inorganic and peptide-based nanomedicines. Therefore, thorough knowledge of these intelligent nanomedicines is crucial for developing very promising drug delivery systems. Polymeric micelles are often simple to make and have high solubilization characteristics; as a result, they seem to be a promising alternative to other nanosystems. Even though recent studies have provided an overview of polymeric micelles, here we included a discussion on the “intelligent” drug delivery from these systems. We also summarized the state-of-the-art and the most recent developments of polymeric micellar systems with respect to cancer treatments. Additionally, we gave significant attention to the clinical translation potential of polymeric micellar systems in the treatment of various cancers.
Design of Innovative Biocompatible Cellulose Nanostructures for the Delivery and Sustained Release of Curcumin
Authors: Casanova Francisca , Pereira Carla F. , Ribeiro Alessandra B. , Costa Eduardo M. , Freixo Ricardo , Castro Pedro M. , Fernandes João C. , Pintado Manuela , Ramos Óscar L.
Published: March 2023

Poor aqueous solubility, stability and bioavailability of interesting bioactive compounds is a challenge in the development of bioactive formulations. Cellulose nanostructures are promising and sustainable carriers with unique features that may be used in enabling delivery strategies. In this work, cellulose nanocrystals (CNC) and cellulose nanofibers were investigated as carriers for the delivery of curcumin, a model liposoluble compound. Nanocellulose modification with the surfactant cetyltrimethylammonium bromide (CTAB), tannic acid and decylamine (TADA), and by TEMPO-mediated oxidation were also tested and compared. The carrier materials were characterized in terms of structural properties and surface charge, while the delivery systems were evaluated for their encapsulation and release properties. The release profile was assessed in conditions that mimic the gastric and intestinal fluids, and cytotoxicity studies were performed in intestinal cells to confirm safe application. Modification with CTAB and TADA resulted in high curcumin encapsulation efficiencies of 90 and 99%, respectively. While no curcumin was released from TADA-modified nanocellulose in simulated gastrointestinal conditions, CNC-CTAB allowed for a curcumin-sustained release of ca. 50% over 8 h. Furthermore, the CNC-CTAB delivery system showed no cytotoxic effects on Caco-2 intestinal cells up to 0.125 g/L, meaning that up to this concentration the system is safe to use. Overall, the use of the delivery systems allowed for the reduction in the cytotoxicity associated with higher curcumin concentrations, highlighting the potential of nanocellulose encapsulation systems.