bluuebunny/crossref_metadata_2025 huggingface dataset:
Alphavirus-induced transcriptional and translational shutoffs play major roles in blocking the formation of stress granules
Authors: Palchevska Oksana , Dominguez Francisco , Frolova Elena I. , Frolov Ilya
Published: July 2023

Alphavirus infections cause multiple alterations in the intracellular environment that can have both positive and negative effects on viral replication. The Old World alphaviruses, such as Sindbis (SINV), chikungunya (CHIKV), and Semliki Forest viruses, hinder the ability of vertebrate cells to form stress granules (SGs). Previously, this inhibitory function was attributed to the hypervariable domain (HVD) of nsP3, which sequesters the key components of SGs, G3BP1 and G3BP2, and to the nsP3 macro domain. The macro domain possesses ADP-ribosylhydrolase activity, which can diminish the ADP-ribosylation of G3BP1 during viral replication. However, our recent findings do not support the prevailing notions. We demonstrate that the interactions between SINV– or CHIKV-specific nsP3s and G3BPs, and the ADP-ribosylhydrolase activity are not major contributors to the inhibitory process, at least when nsP3 is expressed at biologically relevant levels. Instead, the primary factors responsible for suppressing SG formation are virus-induced transcriptional and translational shutoffs that rapidly develop within the first few hours post infection. Poorly replicating SINV variants carrying mutated nsP3 HVD still inhibit SG development even in the presence of NaAs. Conversely, SINV mutants lacking transcription and/or translation inhibitory functions lose their ability to inhibit SGs, despite expressing high levels of wt nsP3. Moreover, we found that stable cell lines expressing GFP-nsP3 fusions retain the capacity to form SGs when exposed to sodium arsenite. However, our results do not rule out a possibility that additional virus-induced changes in cell biology may contribute to the suppression of SG formation.

Importance

Our study highlights the mechanisms behind the cell’s resistance to SG formation after infection with Old World alphaviruses. Shortly after infection, the replication of these viruses hinders the cell’s ability to form SGs, even when exposed to chemical inducers such as sodium arsenite. This resistance is primarily attributed to virus-induced transcriptional and translational shutoffs, rather than interactions between the viral nsP3 and the key components of SGs, G3BP1/2, or the ADP-ribosylhydrolase activity of nsP3 macro domain. While interactions between G3BP and nsP3 are essential for the formation of viral replication complexes, their role in regulating SG development appears to be minimal, if any. Cells harboring replicating virus-specific RNA with modified abilities to inhibit transcription and/or translation, but encoding wt nsP3, retain the capacity for SG development. Understanding these mechanisms of regulation of SG development contributes to our knowledge of viral replication and the intricate relationships between alphaviruses and host cells.
Evaluation of AlphaFold Antibody-Antigen Modeling with Implications for Improving Predictive Accuracy
Authors: Yin Rui , Pierce Brian G.
Published: July 2023

High resolution antibody-antigen structures provide critical insights into immune recognition and can inform therapeutic design. The challenges of experimental structural determination and the diversity of the immune repertoire underscore the necessity of accurate computational tools for modeling antibody-antigen complexes. Initial benchmarking showed that despite overall success in modeling protein-protein complexes, AlphaFold and AlphaFold-Multimer have limited success in modeling antibody-antigen interactions. In this study, we performed a thorough analysis of AlphaFold’s antibody-antigen modeling performance on 429 nonredundant antibody-antigen complex structures, identifying useful confidence metrics for predicting model quality, and features of complexes associated with improved modeling success. We show the importance of bound-like component modeling in complex assembly accuracy, and that the current version of AlphaFold improves near-native modeling success to over 30%, versus approximately 20% for a previous version. With this improved success, AlphaFold can generate accurate antibody-antigen models in many cases, while additional training may further improve its performance.
RegulatingPCCAgene expression by modulation of pseudoexon splicing patterns to rescue enzyme activity in propionic acidemia
Authors: Petersen Ulrika Simone Spangsberg , Dembic Maja , Martínez-Pizarro Ainhoa , Richard Eva , Holm Lise Lolle , Havelund Jesper Foged , Doktor Thomas Koed , Larsen Martin Røssel , Færgeman Nils J. , Desviat Lourdes Ruiz , Andresen Brage Storstein
Published: July 2023

Pseudoexons are nonfunctional intronic sequences that can be activated by deep intronic sequence variation. Activation increases pseudoexon inclusion in mRNA and interferes with normal gene expression. ThePCCAc.1285-1416A>G variation activates a pseudoexon and causes the severe metabolic disorder, propionic acidemia, by deficiency of the propionyl-CoA carboxylase enzyme encoded byPCCAandPCCB. We characterized this pathogenic pseudoexon activation event in detail and identified hnRNP A1 to be important for normal repression. ThePCCAc.1285-1416A>G variation disrupts an hnRNP A1-binding splicing silencer and simultaneously creates a splicing enhancer. We demonstrate that blocking this region of regulation with splice-switching antisense oligonucleotides restores normal splicing and rescues enzyme activity in patient fibroblasts and in a cellular model created by CRISPR gene editing. ThePCCApseudoexon can be exploited as a gene-regulatory switch, as healthy tissues show relatively high levels of inclusion. By blocking inclusion of the non-activated wild type pseudoexon, we increase both PCCA and PCCB protein levels, which increases the activity of the heterododecameric enzyme. Surprisingly, we can increase enzyme activity from residual levels not only in patient fibroblasts harboringPCCAmissense variants, but also those harboringPCCBmissense variants. This could be a potential treatment strategy for propionic acidemia.
Alterations in grey matter structure linked to frequency-specific cortico-subcortical connectivity in schizophrenia via multimodal data fusion
Authors: Duda Marlena , Faghiri Ashkan , Belger Aysenil , Bustillo Juan R. , Ford Judith M. , Mathalon Daniel H. , Mueller Bryon A. , Pearlson Godfrey D. , Potkin Steven G. , Preda Adrian , Sui Jing , Van Erp Theo G.M. , Calhoun Vince D.
Published: July 2023

Schizophrenia (SZ) is a complex psychiatric disorder that is currently defined by symptomatic and behavioral, rather than biological, criteria. Neuroimaging is an appealing avenue for SZ biomarker development, as several neuroimaging-based studies comparing individuals with SZ to healthy controls (HC) have shown measurable group differences in brain structure, as well as functional brain alterations in both static and dynamic functional network connectivity (sFNC and dFNC, respectively). The recently proposed filter-banked connectivity (FBC) method extends the standard dFNC sliding-window approach to estimate FNC within an arbitrary number of distinct frequency bands. The initial implementation used a set of filters spanning the full connectivity spectral range, providing a unified approach to examine both sFNC and dFNC in a single analysis. Initial FBC results found that individuals with SZ spend more time in a less structured, more disconnected low-frequency (i.e., static) FNC state than HC, as well as preferential SZ occupancy in high-frequency connectivity states, suggesting a frequency-specific component underpinning the functional dysconnectivity observed in SZ. Building on these findings, we sought to link such frequency-specific patterns of FNC to covarying data-driven structural brain networks in the context of SZ. Specifically, we employ a multi-set canonical correlation analysis + joint independent components analysis (mCCA + jICA) data fusion framework to study the connection between grey matter volume (GMV) maps and FBC states across the full connectivity frequency spectrum. Our multimodal analysis identified two joint sources that captured co-varying patterns of frequency-specific functional connectivity and alterations in GMV with significant group differences in loading parameters between the SZ group and HC. The first joint source linked frequency-modulated connections between the subcortical and sensorimotor networks and GMV alterations in the frontal and temporal lobes, while the second joint source identified a relationship between low-frequency cerebellar-sensorimotor connectivity and structural changes in both the cerebellum and motor cortex. Together, these results show a strong connection between cortico-subcortical functional connectivity at both high and low frequencies and alterations in cortical GMV that may be relevant to the pathogenesis and pathophysiology of SZ.
Recent emergence of cephalosporin resistantSalmonellaTyphi in India due to the endemic clone acquiring IncFIB(K) plasmid encodingblaCTX-M-15gene
Authors: Priya T Tharani , Jacob Jobin John , Aravind V , Priya T Monisha , Shah Bhavini , Iyer Veena , Maheshwari Geeti , Trivedi Urmi , Shah Anand , Patel Pooja , Gaigawale Anushree , Yesudoss M , Narayanan Pavithra Sathya , Mutreja Ankur , Carey Megan , John Jacob , Kang Gagandeep , Veeraraghavan Balaji
Published: July 2023

The emergence and spread ofSalmonellaTyphi (S. Typhi) resistant to third-generation cephalosporins is a serious global health concern. In this study, we genomically characterized 142 cephalosporin-resistantS. Typhi strains isolated from India. Comparative genome analysis revealed the emergence of a new clone of ceftriaxone-resistantS. Typhi harboring three plasmids of the incompatibility groups IncFIB(K), IncX1, and IncFIB(pHCM2). Among these, the IncFIB(K) plasmid confers resistance to third-generation cephalosporins through theblaCTX-M-15gene, along with other resistance determinants such as aph(3“), aph(6’),sul2,dfrA14, qnrS,andtetA. Phylogenetic analysis showed that the isolates from Gujarat (n=140/142) belong to a distinct subclade (genotype 4.3.1.2.2) within genotype 4.3.1.2 (H58 lineage II). SNP-based phylogenetic analysis of the core genes in IncFIB(K) reveals a close genetic relationship between the plasmid backbone and that of IncFIB(K) from other Enterobacterales, suggesting that the H58 lineage II possesses the capability to acquire MDR plasmids from these organisms. This could indicate the potential onset of a new wave of ceftriaxone-resistantS.Typhi in India. The implementation of control measures such as vaccination, and improved water, sanitation, and hygiene (WASH) systems, is crucial in areas where MDR or XDRS. Typhi strains are prevalent to curb the spread and impact of these resistant strains.

Importance

Typhoid fever remains a significant public health problem in many parts of the world, particularly in resource-limited settings with inadequate sanitation and limited access to clean water. The emergence of MDRS. Typhiresistant to first-line antibiotics (chloramphenicol, ampicillin, and cotrimoxazole) and fluoroquinolones marked the initial treatment challenge, leading to reliance on azithromycin and third-generation cephalosporins. Recently, researchers identified the emergence of XDR typhoid in Pakistan that is resistant to five classes of antibiotics including third-generation cephalosporins. In this study, we report a recent emergence ofS. Typhi isolates resistant to third-generation cephalosporins. Genomic characterization and evolutionary analysis of the new strains revealed a recent acquisition ofblaCTX-Mcarrying IncFIB(K) plasmid led to the emergence of ceftriaxone-resistantS.Typhi. Due to the ongoing nature of this outbreak, the data from this study deserves further consideration in order to control its spread in India.
Fus3 interacts with Gal83, revealing the MAPK crosstalk to Snf1/AMPK to regulate secondary metabolic substrates in filamentous fungi
Authors: Ma Longxue , Xing Fuguo , Li Xu , Tai Bowen , Guo Ling
Published: July 2023

The pheromone MAPK is essential for the vital activities of fungi and is widely identified in filamentous fungi of agricultural, medical, and industrial relevance. The targets have rarely been reported and it is difficult to understand the mechanism of pheromone MAPK signaling pathway. Aflatoxins (AFs), highly carcinogenic natural products, are produced by the secondary metabolism of fungi, such asAspergillus flavus. Our previous studies demonstrated that Fus3 regulates AFs by modulating substrate levels inAspergillus flavus, but no mechanism explain that in fungi. Here we show Gal83, a new target of Fus3, and identified the pheromone Fus3-MAPK signaling pathway regulates the Snf1/AMPK energy-sensing pathway to modulate aflatoxins synthesis substrates. In the screening for target proteins of Fus3, the Snf1/AMPK complexes β subunit was identified by using tandem affinity purification and multi-omics, which physically interacted with Fus3 invivoandvitroand received phosphorylation from Fus3. While neither aflatoxin transcript levels were down-regulated ingal83-mutant andfus3-mutant strains, significant decreases in aflatoxin B1, aflatoxin synthetic substrates levels and gene expression levels of primary metabolic enzymes were shown that both the Fus3-MAPK and Snf1/AMPK pathways could response energy signal. In conclusion, all the evidence unlocks a novel pathway of Fus3-MAPK to regulate AFs synthesis substrates by cross-talking to the Snf1/AMPK complexes.

Importance

Aflatoxin poses a great threat to human and animal health and the economy, thus the mechanisms regulating aflatoxin synthesis have been of great interest. We have previously demonstrated that MAPK regulates aflatoxin biosynthesis significantly, but the regulatory mechanism of Fus3-MAPK is not clear. Here we found that Pheromone Fus3-MAPK responds to energy and transmits to Snf1/AMPK through phosphorylation, which regulates the level of secondary metabolic substrates inAspergillus flavus, as a novel pathway of Fus3-MAPK. Fus3 interacts stably with Gal83 and colocalizes in the cytoplasm and nucleus, directly regulating the levels of aflatoxin synthetic substrates. These data advance our understanding of the regulation of aflatoxin by pheromone MAPK, and the mechanism of pheromone MAPK and Snf1/AMPK crosstalk regulation is confirmed. Overall, this has a positive effect on both fungal regulatory mechanisms and aflatoxin prevention and control.
A genetic code change in progress: tRNA-Leu(CAG) is conserved in mostSaccharomycopsisyeast species but is non-essential and does not compete with tRNA-Ser(CAG) in translation
Authors: Cinnéide Eoin Ó , Scaife Caitriona , Dillon Eugene , Wolfe Kenneth H.
Published: July 2023

In the yeast generaSaccharomycopsisandAscoidea, nuclear genes use a non-standard genetic code in which CUG codons are translated as serine instead of leucine, due to a tRNA-Ser with the unusual anticodon CAG. However, some species in this ‘CUG-Ser2’ clade also contain an ancestral tRNA-Leu gene with the same anticodon. One of these species,Ascoidea asiatica, has been shown to have a stochastic proteome in which proteins contain approximately 50% Ser and 50% Leu at CUG codon sites, whereas previously examinedSaccharomycopsisspecies translate CUG only as Ser. Here, we investigated the presence, conservation, and possible functionality of the tRNA-Leu(CAG) gene in the genusSaccharomycopsis. We analyzed the genomes of 33 strains, including almost all known species ofSaccharomycopsis, and found that most of them contain both tRNA-Ser(CAG) and tRNA-Leu(CAG) genes. The tRNA-Leu(CAG) gene is evolving faster than tRNA-Ser(CAG) and it has been lost in two species,S. microsporaandS. synnaedendra. We deleted the single tRNA-Leu(CAG) gene inS. capsularisand found that it is not essential. Bioinformatic analysis suggested that some CUG codon sites inSaccharomycopsisspecies may be translated as Leu, specifically in genes with functions in meiosis or sporulation, but mass spectrometry of sporulatingS. capsularisandS. fermentanscultures showed only CUG-Ser translation. Cloverleaf structures of tRNA-Leu(CAG) from allSaccharomycopsisspecies contain mutations that are likely to make them non-functional in translation, but the evolutionary conservation of the gene leads us to propose that it has been retained for an unknown non-translational role.
Augmented Super-Resolution Radial Fluctuations (aSRRF) Pushing the Limits of Structured Illumination Microscopy
Authors: Zhang Heng , Wang Jianhang , Jin Luhong , Zhu Yunqi , Guo Yuting , Zhang Meng , Zhang Yuhui , Wang Zhixiong , Su Yisun , Wu Yicong , Ji Baohua , Toomre Derek , Liu Xu , Xu Yingke
Published: July 2023

Structured illumination microscopy (SIM) is a versatile super-resolution technique known for its compatibility with a wide range of probes and fast implementation. While 3D SIM is capable of achieving a spatial resolution of ∼120 nm laterally and ∼300 nm axially, attempting to further enhance the resolution through methods such as nonlinear SIM or 4-beam SIM introduces complexities in optical configurations, increased phototoxicity, and reduced temporal resolution.

Here, we have developed a novel method that combines SIM with augmented super-resolution radial fluctuations (aSRRF) utilizing a single image through image augmentation. By applying aSRRF reconstruction to SIM images, we can enhance the SIM resolution to ∼50 nm isotopically, without requiring any modifications to the optical system or sample acquisition process. Additionaly, we have incorporated the aSRRF approach into an ImageJ plugin and demonstrated its versatility across various fluorescence microscopy images, showcasing a remarkable two-fold resolution increase.
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release relies on the integrity of the C-terminal half of the SNARE domain of Syntaxin-1A
Authors: Salazar-Lázaro Andrea , Trimbuch Thorsten , Vardar Gülçin , Rosenmund Christian
Published: July 2023

The SNARE proteins are central in membrane fusion and, at the synapse, neurotransmitter release. However, their involvement in the dual regulation of the synchronous release while maintaining a pool of readily releasable vesicles remains unclear. Using a chimeric approach, we performed a systematic analysis of the SNARE domain of STX1A by exchanging the whole SNARE domain or its N- or C-terminus subdomains with those of STX2. We expressed these chimeric constructs in STX1-null hippocampal mouse neurons. Exchanging the C-terminal half of STX1’s SNARE domain with that of STX2 resulted in a reduced RRP accompanied by an increased release rate, while inserting the C-terminal half of STX1’s SNARE domain into STX2 lead to an enhanced priming and decreased release rate. Additionally, we found that the mechanisms for clamping spontaneous, but not for Ca2+-evoked release, are particularly susceptible to changes in specific residues on the outer surface of the C-terminus of the SNARE domain of STX1A. Particularly, mutations of D231 and R232 affected the fusogenicity of the vesicles. We propose that the C-terminal half of the SNARE domain of STX1A plays a crucial role in the stabilization of the RRP as well as in the clamping of spontaneous synaptic vesicle fusion through the regulation of the energetic landscape for fusion, while it also plays a covert role in the speed and efficacy of Ca2+-evoked release.
Structural and biochemical insights into purine-based drug molecules in hBRD2 delineate a unique binding mode opening new vistas in the design of inhibitors of the BET family
Authors: Arole Aishwarya H. , Deshmukh Prashant , Sridhar Ashok , Mathur Shruti , Mahalingaswamy Mahesh , Subramanya Hosahalli , Dalavaikodihalli Nanjaiah Nandakumar , Padmanabhan Balasundaram
Published: July 2023

The bromodomain and extra-terminal (BET) family proteins, which are involved in chromatin function, have been shown to be promising drug targets in several pathological conditions, including cancer and inflammation. There is considerable interest in the development of BET inhibitors with novel scaffolds to modulate the epigenesis of such diseases. Here, high-resolution crystal structures of the purine class of FDA-approved drugs (theophylline, doxophylline and acyclovir) and non-FDA-approved compounds (3-methyl-7-propylxanthine and theobromine) complexed with hBRD2 bromodomains BD1 and BD2 are reported. Remarkably, a new binding site is exhibited by stacking the compounds against the WPF shelf of BD1 and BD2. This serendipitous binding, in addition to the known acetyl-lysine binding site, sufficiently anchors the ligands in the solvent-exposed region. In addition, slight variations in the lipophilicity of these molecules significantly affected the in vitro binding affinity and selectivity towards BD1 compared with BD2. This idiosyncratic binding provides a new structural framework to link these sites for the development of next-generation inhibitors of the BET family.
Work–life balance in the construction industry: a bibliometric and narrative review
Authors: Adah Christiana Ada , Aghimien Douglas Omoregie , Oshodi Olalekan
Published: July 2023

Purpose

The nature of construction works has a negative impact on physical, mental and emotional well-being and makes it difficult for the construction industry to attack and retain its workforce. The current study seeks to integrate the current knowledge focused on work–life balance (WLB) in the industry into an understandable whole.

Design/methodology/approach

An interpretivist philosophical approach was adopted using a bibliometric review and a narrative review of existing studies from both Scopus and Google databases. The Visualisation of Similarities viewer (VOSviewer) was used to prepare co-occurrence maps from the bibliographic data garnered.

Findings

The study reveals that the prominent factors influencing the WLB of the construction workforce are organizational culture, salary earned, heavy workload, long working hours and inflexible working time. The recent WLB discourse is on organisational commitment, job satisfaction and workplace dynamics. While WLB areas for further exploration are job stress, safety performance, employee attrition and an ageing workforce. Meanwhile, Africa and South America are still lagging in WLB research.

Practical implications

The findings reported here will assist stakeholders in identifying appropriate WLB initiatives that can be used to improve the well-being of the construction workforce. Also, the gaps in knowledge for further research were highlighted.

Originality/value

The findings reveal current trends and a road map for future studies on WLB in construction. It also reveals prominent factors influencing the WLB of workers in the construction industry.
Incorporating counterproductive knowledge behaviour in the higher education context: proposing the potential remedies in explaining the faculty members’ performance
Authors: Zamrudi Zakky , Setiawan Margono , Irawanto Dodi Wirawan , Rahayu Mintarti
Published: July 2023

Purpose

This study aims to seek to understand counterproductive knowledge behaviour (CKB) in higher education institutions (HEIs) by integrating knowledge hiding (KHi) and lack of knowledge sharing (LKS).

Design/methodology/approach

The current research uses a hybrid conceptual review combining the bibliometric study and conceptual review of 47 articles. The bibliometric analysis aimed to identify research maps, and the conceptual review sought to understand the current development of research fields.

Findings

The bibliometric analysis highlights essential summaries, such as the pioneering authors, seminal papers and conceptual maps. However, knowledge hoarding as a dimension of LKS appears in the niche theme. The conceptual analysis indicates three groups of factors contributing to KHi and LKS. Moreover, the study highlights the causal relation between both KHi as well as LKS and faculty member performance while proposing remedies derived from integrating the social exchange theory (SET) and conservation of resource (COR) theory.

Research limitations/implications

The present study provides an integrated image of KHi and LKS in the HEI context, as well as its potential remedies by integrating SET and COR. The basis of this study is a literature review; thus, future studies are recommended to empirically explore the integration of KHi and LKS within the HEI context.

Practical implications

This research provides an overview for HEI policymakers to re-examine the critical role of institutional research as one of the considerations for evaluating and developing policies. Specifically, policymakers can reflect on all policy directions to determine whether there are any symptoms of CKB in the institutions. Moreover, stakeholders can find out the reason behind the non-optimal performance of faculty members regarding CKB and any mitigating factors.

Originality/value

Understanding CKB is crucial in managing HEI. This research provides a comprehensive image of KHi and LKS within HEI, especially in a collectivist culture.
Does religiosity matter in the workplace? A moderated-mediated examination of abusive supervision, revenge and deviance
Authors: Ahmad Saima , Dar Nasib , Rahman Wali
Published: July 2023

Purpose

This paper aims to investigate the influence of religiosity on the relationship between abusive supervision and deviant work behavior (DWB). This paper examines whether the desire for revenge mediates the relationship between abusive supervision and DWB and whether the strength of this relationship is moderated by religiosity.

Design/methodology/approach

The proposed relationships were examined by collecting primary data from 350 employees using multistage sampling procedures. Hayes’ Process Macro was used to analyze the proposed moderated-mediation model of abusive supervision, DWB, religiosity and desire for revenge.

Findings

The analytical findings indicate that an employee’s desire for revenge mediates the relationship between abusive supervision and DWB. However, religiosity moderates the indirect effect of abusive supervision (mediated by the desire for revenge) on DWB.

Originality/value

To the best of the authors’ knowledge, this is the first study that explores the role of individual-level religiosity in mitigating the harmful effects of abusive supervision on deviant behavior and revenge in the workplace.
Forecasting law enforcement frequency of internet+ coal mine safety supervision
Authors: Long Yuzhen , Yang Chunli , Li Xiangchun , Lu Weidong , Zhang Qi , Gao Jiaxing
Published: July 2023

Purpose

Coal is the basic energy and essential resource in China, which is crucial to the economic lifeline and energy security of the country. Coal mining has been ever exposed to potential safety risks owing to the complex geologic environment. Effective safety supervision is a vital guarantee for safe production in coal mines. This paper aims to explore the impacts of the internet+ coal mine safety supervision (CMSS) mode that is being emerged in China.

Design/methodology/approach

In this study, the key factors influencing CMSS are identified by social network analysis. They are used to develop a multiple linear regression model of law enforcement frequency for conventional CMSS mode, which is then modified by an analytical hierarchy process to predict the law enforcement frequency of internet+ CMSS mode.

Findings

The regression model demonstrated high accuracy and reliability in predicting law enforcement frequency. Comparative analysis revealed that the law enforcement frequency in the internet+ mode was approximately 40% lower than the conventional mode. This reduction suggests a potential improvement in cost-efficiency, and the difference is expected to become even more significant with an increase in law enforcement frequency.

Originality/value

To the best of the authors’ knowledge, this is one of the few available pieces of research which explore the cost-efficiency of CMSS by forecasting law enforcement frequency. The study results provide a theoretical basis for promoting the internet+ CMSS mode to realize the healthy and sustainable development of the coal mining industry.
Agricultural resources and practices in the circular bioeconomy adoption: evidence from a rural region of Greece
Authors: Papadopoulou Christina-Ioanna , Loizou Efstratios , Chatzitheodoridis Fotios , Karelakis Christos
Published: July 2023

Purpose

This study aims to assess the factors influencing the adoption of circular bioeconomy practices by crop farmers.

Design/methodology/approach

Data collected from 303 farmers in the region of Western Macedonia (WM) were used to identify the adoption factors of the circular bioeconomy. Principal component analysis (PCA) and exploratory factor analysis (EFA) techniques were applied through a custom-designed and specially structured questionnaire.

Findings

The results reveal four main motivations that lead farmers to adopt circular bioeconomy practices in their farming activities: interest in physical-economic resources and factors related to production and consumption; technology and renewable energy and the environment.

Research limitations/implications

This study is based solely on farmers in WM. Furthermore, this study assumes that there will be a strategy to promote a circular bioeconomy under the auspices of the government.

Originality/value

Few studies have focused on the perspectives of crop farmers and what encourages them to adopt circular bioeconomy practices in their crops. Existing barriers are related to economic incentives and constraints. The more circular bioeconomy practices farmers adopt, the greater the positive impact on the environment and rural development, and the factors influencing the adoption of these practices are investigated here.
The data-driven leader: developing a big data analytics leadership competency framework
Authors: Schmidt David Holger , van Dierendonck Dirk , Weber Ulrike
Published: July 2023

Purpose

This study focuses on leadership in organizations where big data analytics (BDA) is an essential component of corporate strategy. While leadership researchers have conducted promising studies in the field of digital transformation, the impact of BDA on leadership is still unexplored.

Design/methodology/approach

This study is based on semi-structured interviews with 33 organizational leaders and subject-matter experts from various industries. Using a grounded theory approach, a framework is provided for the emergent field of BDA in leadership research.

Findings

The authors present a conceptual model comprising foundational competencies and higher order roles that are data analytical skills, data self-efficacy, problem spotter, influencer, knowledge facilitator, visionary and team leader.

Research limitations/implications

This study focuses on BDA competency research emerging as an intersection between leadership research and information systems research. The authors encourage a longitudinal study to validate the findings.

Practical implications

The authors provide a competency framework for organizational leaders. It serves as a guideline for leaders to best support the BDA initiatives of the organization. The competency framework can support recruiting, selection and leader promotion.

Originality/value

This study provides a novel BDA leadership competency framework with a unique combination of competencies and higher order roles.
Framework for sustainable value creation: a synthesis of fragmented sustainable business model literature
Authors: Manninen Kaisa , Laukkanen Minttu , Huiskonen Janne
Published: July 2023

Purpose

This study aims to synthesize the body of sustainable value creation (SVC) research within sustainable business model literature through a systematic literature review.

Design/methodology/approach

A systematic literature review of 85 research articles of SVC through business models from 2011 to 2020.

Findings

The systematic literature review allowed the authors to identify five core SVC elements: value forms, stakeholders, temporal view, spatial view and tensions and conflicts. Moreover, a conceptual framework presenting the interrelationships of the SVC elements is proposed.

Practical implications

This study carries implications for practitioners in the form of guiding questions provided in the framework. Those questions help responsible managers to plan, identify and choose strategic sustainability actions and to develop companies’ business models aiming to lead to the creation of long-term sustainable value in different time frames and locations or different parts of the value network. Additionally, the framework guides managers to identify and manage potential tensions and conflicts which can otherwise hinder SVC.

Originality/value

To the best of the authors’ knowledge, this study is the first systematic literature review of SVC through business models with the conceptual development of SVC. The study synthesizes the fragmented literature to identify SVC elements and build basis for conceptualization of SVC through business models.
Essential 3: professional learning in the context of PDS’ and school-university partnerships
Authors: Polly Drew , Badiali Bernard , Burns Rebecca West , Coler Cynthia , Cosenza Michael , Goree Krystal , Stoicovy Donnan , Zenkov Kristien
Published: July 2023

Purpose

The purpose of this article is to provide a description as well as examples related to Essential 3 in the Second Edition of the NAPDS Nine Essentials.
Regional resilience and the role of cooperative firms
Authors: Costa Michele , Delbono Flavio
Published: July 2023

Purpose

This paper aims to investigate the impact of cooperative firms on the patterns of regional economic resilience in Italy from 2008 to 2019.

Design/methodology/approach

This study uses regional statistics to compute indices of resilience for both real GDP per capita and employment during both recovery and resistance periods. By means of a linear model, the authors investigate the relationships between indices of resilience and the cooperative presence, while controlling for a set of demographic, social and economic variables.

Findings

This study shows that during (and after) recessions such regional indices exhibit very different patterns, with notably poorer performance observed in Southern regions compared to the rest of the country. Furthermore, this study illustrates that the size of the cooperative employment improves the overall resilience of regional employment, especially during recovery periods.

Social implications

The findings hint at policies enhancing the strength and scope of the cooperative movement as a driver of territorial resilience.

Originality/value

To the best of the authors’ knowledge, this is the first study in relating territorial resilience and the presence of a type of companies. This study performs the analysis at the regional level regarding cooperative enterprises. The new findings hint at some policies enhancing the strength and scope of the cooperative movement.
Widespread amphibian Perkinsea infections associated with Ranidae hosts, cooler months and Ranavirus co‐infection
Authors: Atkinson Matthew S. , Savage Anna E.
Published: July 2023

Amphibians suffer from large‐scale population declines globally, and emerging infectious diseases contribute heavily to these declines. Amphibian Perkinsea (Pr) is a worldwide anuran pathogen associated with mass mortality events, yet little is known about its epidemiological patterns, especially in comparison to the body of literature on amphibian chytridiomycosis and ranavirosis.

Here, we establish Pr infection patterns in natural anuran populations and identify important covariates including climate, host attributes and co‐infection with Ranavirus (Rv).

We used quantitative (q)PCR to determine the presence and intensity of Pr and Rv across 1234 individuals sampled throughout central Florida in 2017–2019. We then implemented random forest ensemble learning models to predict infection with both pathogens based on physiological and environmental characteristics.

Perkinsea infected 32% of all sampled anurans, and Pr prevalence was significantly elevated in Ranidae frogs, cooler months, metamorphosed individuals and frogs co‐infected with Rv, while Pr intensity was significantly higher in ranid frogs and individuals collected dead. Ranavirus prevalence was 17% overall and was significantly higher in Ranidae frogs, metamorphosed individuals, locations with higher average temperatures, and individuals co‐infected with Pr. Perkinsea prevalence was significantly higher than Rv prevalence across months, regions, life stages and species. Among locations, Pr prevalence was negatively associated with crayfish prevalence and positively associated with relative abundance of microhylids, but Rv prevalence did not associate with any tested co‐variates. Co‐infections were significantly more common than single infections for both pathogens, and we propose that Pr infections may propel Rv infections because seasonal Rv infection peaks followed Pr infection peaks and random forest models found Pr intensity was a leading factor explaining Rv infections.

Our study elucidates epidemiological patterns of Pr in Florida and suggests that Pr may be under‐recognized as a cause of anuran declines, especially in the context of pathogen co‐infection.
Issuer Motivations for Corporate Green Bond Offerings
Authors: Dutordoir Marie , Li Shuyu , Neto João Quariguasi Frota
Published: July 2023

Despite the substantial increase in green bond issuance over the past decade, evidence on the drivers and costs of corporate green bond issuance is lacking. We develop four novel hypotheses on the determinants of firms’ choice between green and conventional bonds, by connecting the four distinct features of green bonds with relevant theories. We test these hypotheses on a sample of green and conventional bonds issued by US, Western European and Chinese firms between 2014 and 2021, using linear probability models and alternative approaches. Consistent with our hypotheses, we find that firms with higher reputational gains from being seen as green and a stronger focus on eco‐innovation are more likely to issue green instead of conventional bonds. Conversely, we obtain only limited evidence that green bond issuance is driven by the net benefits of additional disclosure and no evidence that green bond issuers cater to time‐varying investor preferences for corporate greenness. Our results, which survive several robustness tests, are relevant for corporate managers, investors and bond market regulators.
Untangling human resource management and employee wellbeing relationships: Differentiating job resource HR practices from challenge demand HR practices
Authors: Li Mengwei , Fu Na , Chadwick Clint , Harney Brian
Published: July 2023

In the strategic HR literature, current empirical results on the relationship between HR practices and employee wellbeing are mixed and contradictory. Based on the job resources and demands model and the fine‐tuned challenge‐hindrance demands framework, we propose that an important reason lies in the lack of attention paid to the different characteristics of HR practices. HR practices can serve as either job resources or challenge demands to employees, thereby having differential effects on the psychological, physical, and social dimensions of wellbeing. We integrate a measure of challenge demand (including time pressure and workload) as a mediator to further reveal how these different categories of HR practices influence employee wellbeing. Using structural equation modeling in a dataset of 4823 individual workers from a National Workplace Survey of Employees conducted in Ireland, we find that job resource HR practices are positively associated with all three dimensions of wellbeing both directly and indirectly, while challenge demand HR practices are positively associated with psychological wellbeing but negatively associated with physical wellbeing and social wellbeing primarily through the mediating effect of time pressure and workload. These findings point to important variable relationships, reinforcing the need to untangle the HRM employee wellbeing relationship beyond aggregated and uniform HRM‐wellbeing assertions.
Teaching through the cloud: An ethnography of the role of cloud‐based collaborative technologies in the formation of teachers' classroom practices
Authors: Unsworth Ruth
Published: July 2023

Through an examination of ethnographic fieldwork data, this paper explores the ways in which cloud‐based collaborative technologies created by Google mediate (Latour 1994) teachers' discussions around, agreement of and enactments of their classroom practices. Bringing together concepts from actor‐network theory and literacy studies, this paper argues for greater consideration of the role(s) of increasingly‐used technologies in shaping teachers' practices and suggests a framework for exploring this issue further.
The double‐edged sword of employee forgiveness: How forgiveness motives steer forgiveness toward interpersonal citizenship behaviors and interpersonal deviance
Authors: Zhang Junwei , Zhang Yajun , Lu Lu
Published: July 2023

Previous research has almost universally shown that forgiveness is a beneficial virtue that can generate a series of positive outcomes. We challenge this prevailing view by proposing that employee forgiveness is a mixed blessing. Setting off from distinguishing the motives behind forgiveness, we integrated the relational perspective and ego depletion theory to explore the beneficial and detrimental consequences of employee forgiveness. Specifically, our study investigated when and how employee forgiveness leads to interpersonal citizenship behaviors (ICBs) and interpersonal deviance. Using a sample of 349 supervisor–employee dyads, we found that employee forgiveness driven by strong relationship‐oriented motives could foster high‐quality relationships with coworkers and facilitate ICBs. In contrast, employee forgiveness driven by strong self‐oriented motives could cause more resource depletion and increase interpersonal deviance. Furthermore, we discuss the theoretical and practical implications of our study as well as future research directions.
Adopting telework: The causal impact of working from home on subjective well‐being
Authors: Gueguen Guillaume , Senik Claudia
Published: July 2023

We study the impact of work from home (WFH) on subjective well‐being during the Covid period, where self‐selection of individuals into telework is ruled out, at least part of the time, by stay‐at‐home orders. We use a difference‐in‐differences approach with individual fixed effects and identify the specific impact of switching to telecommuting, separately from any other confounding factor. In particular, our identification strategy avoids the influence of interpersonal heterogeneity by exploiting the multiple entries into WFH, by the same individuals, at different times. On average over the period, switching to WFH, especially full‐time, worsens mental health. We also find a positive but imprecisely measured impact of part‐time WFH on life satisfaction. However, this hides a dynamic evolution, whereby the initial deterioration gives place to an adaptation process after a couple of months. We also uncover a particularly pronounced fall in subjective well‐being of women with children, especially in the first months; this could be associated with home‐schooling.
Connection between oxidative stress and subcellular organelle in subarachnoid hemorrhage: Novel mechanisms and therapeutic implications
Authors: Zhang Jiahao , Zhang Zeyu , Wang Xiaoyu , Liu Yibo , Yu Qian , Wang Kaikai , Fang Yuanjian , Lenahan Cameron , Chen Maohua , Chen Sheng
Published: July 2023

Spontaneous subarachnoid hemorrhage (SAH) is one of the most devastating forms of stroke, with limited treatment modalities and poor patient outcomes. Previous studies have proposed multiple prognostic factors; however, relative research on treatment has not yet yielded favorable clinical outcomes. Moreover, recent studies have suggested that early brain injury (EBI) occurring within 72 h after SAH may contribute to its poor clinical outcomes. Oxidative stress is recognized as one of the main mechanisms of EBI, which causes damage to various subcellular organelles, including the mitochondria, nucleus, endoplasmic reticulum (ER), and lysosomes. This could lead to significant impairment of numerous cellular functions, such as energy supply, protein synthesis, and autophagy, which may directly contribute to the development of EBI and poor long‐term prognostic outcomes. In this review, the mechanisms underlying the connection between oxidative stress and subcellular organelles after SAH are discussed, and promising therapeutic options based on these mechanisms are summarized.
Cost‐effectiveness of canagliflozin and dapagliflozin for treatment of patients with chronic kidney disease and type 2 diabetes
Authors: Nguyen Bao‐Ngoc , Mital Shweta , Bugden Shawn , Nguyen Hai V.
Published: July 2023

Aim

To examine the cost‐effectiveness of adding canagliflozin or dapagliflozin to standard of care (SoC) versus SoC alone in patients with chronic kidney disease (CKD) and type 2 diabetes (T2D).

Materials and Methods

We used a Markov microsimulation model to assess the cost‐effectiveness of canagliflozin plus SoC (canagliflozin + SoC), dapagliflozin plus SoC (dapagliflozin + SoC) and SoC alone. Analyses were conducted from a healthcare system perspective. Costs were measured in 2021 Canadian dollars (C$), and effectiveness was measured in quality‐adjusted life‐years (QALYs).

Results

Over a patient's lifetime, canagliflozin + SoC and dapagliflozin + SoC yielded cost savings of C$33 460 and C$26 764 and generated 1.38 and 1.44 additional QALYs compared with SoC alone, respectively. While QALY gains with dapagliflozin + SoC were higher than those with canagliflozin + SoC, this strategy was also more costly with the incremental cost‐effectiveness ratio exceeding the willingness to pay threshold of C$50 000 per QALY. Dapagliflozin + SoC, however, generated cost savings and QALY gains compared with canagliflozin + SoC over shorter time horizons of 5 or 10 years.

Conclusions

Dapagliflozin + SoC was not cost‐effective versus canagliflozin + SoC in patients with CKD and T2D over the lifetime horizon. However, adding canagliflozin or dapagliflozin to SoC was less costly and more effective relative to SoC alone for treatment of CKD and T2D.
Comparison of physicochemical and functional characteristics of insoluble dietary fibre from okara by separate and co‐fermentation with lactic acid bacteria and Kluyveromyces marxianus
Authors: Yue Yang , Li Yunbo , Li Pengcheng , Xiao Yuyi , Piao Chunhong , Wang Xiujuan
Published: July 2023

Okara, a natural source of dietary fibre (DF), is recognised as an edible and safe material. In this study, lactic acid bacteria (LAB) and Kluyveromyces marxianus were used to ferment okara in separately or in combination, and the physicochemical and functional characteristics of obtained insoluble dietary fibre (IDF) excluded from okara were compared. The result showed that compared with non‐fermented okara, the content of IDF from co‐fermented okara by LAB and K. marxianus increased by 6.8%, while separate fermentations were 2.4% and 4.0%. Scanning electron microscopy result showed that the structure of co‐fermented okara IDF was looser and porous with the edge disappeared compared to separate and non‐fermented okara. All the fermentation treatment groups effectively reduced the particle size and affected the cellulose and hemicellulose functional groups of IDF. Furthermore, compared with non‐fermented okara, processing performance, and the activities of hypoglycaemic and hypolipidaemic of IDF were greatly improved in fermented okara.