bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics of Job Satisfaction for Employees of SMEs in Shan Dong, China
Authors: Sun Yuxuan , Traiwannakij Surachai
Published: November 2024

With the rise of domestic innovation and employees, the number of small and medium-sized enterprises in China has increased yearly, providing many jobs, making many contributions to maintain social stability, promoting social and economic development, and occupying an essential position in the national economy. However, there is little research on the SME market in Shandong, China. This study, therefore, aims to investigate the factors influencing Job Satisfaction for SME employees in Shand Dong. The quantitative method based on questionnaires is applied. Descriptive statistics such as frequency, percent frequency, arithmetic mean, and standard deviation are introduced. Various inferential statistical methods are used to test the hypothesis, particularly the Independent Samples t-test, the One-way ANOVA, and the Multiple Linear Regression Analysis. The results obtained from the study indicate that differences in all aspects of Demographic Factors, namely, Gender, Marital Status, Age, and Educational Level, generate differences in Job Satisfaction. Different aspects of Business Characteristics, namely, Number of Employees, Types of Business, Business Method, Hours Spent Per Week, and Business Development Stage, generate differences in Job Satisfaction based on the Independent Samples t-test and the One-way ANOVA statistics. The results obtained from the Multiple Linear Regression Analyses show significant positive impacts on some aspects of Job Characteristics: Task Identity, Task Significance, and Feedback on Job Satisfaction.
Near-Time Digital Mapping for Geoforensic Searches
Authors: Rocke Benjamin , Ruffell Alastair
Published: November 2024

Inadequate base maps with poor scale or low resolution demonstrate the need for contemporary topographic maps when conducting geological mapping. In neotectonic regimes and areas of dynamic geomorphology, archival or large-scale maps require time-consuming, on-site manual updating while mapping bedrock and superficial geology. In contrast, stable ground conditions may have suitable legacy maps in some locations but not in others, such as where surveying is absent, incomplete or subject to legal restrictions. The geologist tasked with mapping may have to do this on short notice at their first site visit with no time to search for or create digital or physical copies of background maps on a suitable scale. The field mapper may encounter any of the above scenarios, especially the Geoforensic specialist tasked with Search and Rescue, hazard assessment or preparation and desktop study for subsequent search teams or law enforcement. Drone-derived orthoimagery and digital surface modelling can be conducted on-site in near real time to provide high resolution georeferenced maps for direct input of geological information, thus bypassing either non-existent or unsuitable base maps.
Shear wave elastography based analysis of changes in fascial and muscle stiffness in patients with chronic non-specific low back pain
Authors: Liu Kun , Zhao Tong , Zhang Yang , Chen Lili , Zhang Haoran , Xu Xiqiang , Yuan Zenong , Zhang Qingyu , Dong Jun
Published: November 2024

Background

The quantitative assessment of individual muscle and fascial stiffness in patients with low back pain remains a challenge. This study aimed to compare the stiffness of the thoracolumbar fascia (TLF), erector spinae (ES), and multifidus (MF) in patients with and without chronic non-specific low back pain (CNLBP) using shear wave elastography (SWE). It also sought to explore the relationship between muscle and fascial stiffness and the levels of pain and dysfunction in patients with CNLBP.

Methods

In this cross-sectional study, 30 patients with CNLBP (age 27.40 ± 4.57 years, 19 males, 11 females, BMI 22.96 ± 2.55 kg/m2) and 32 healthy controls (age 27.94 ± 4.94 years, 15 males, 17 females, BMI 22.52 ± 2.26 kg/m2) were enrolled. Stiffness of the TLF, ES, and MF was measured using SWE, and Young’s modulus values were recorded. The numeric rating scale (NRS) for quantifying pain intensity and the Oswestry Disability Index (ODI) scores were recorded for the case group to examine their correlations with the resilience index.

Results

The CNLBP group exhibited significantly higher shear modulus values at the L4-5 bilateral TLF (left: p = 0.014, d = 0.64; right: p = 0.002, d = 0.86), ES (left: p = 0.013, d = 0.66; right: p = 0.027, d = 0.58), and MF (left: p = 0.009, d = 0.69; right: p = 0.002, d = 0.85) compared to the control group. Comparable findings were observed for the right ES (p = 0.026, d = 0.59) and left MF (p = 0.020, η2 = 0.09) at L1-2. Strong correlations were observed between the shear modulus of the bilateral TLF (left: r = 0.57, p = 0.001; right: r = 0.65, p < 0.001) at L4-5 and the NRS scores. Moderate correlations were noted between the shear modulus of the ES (left: r = 0.42, p = 0.022; right: r = 0.48, p = 0.007) and MF (left: r = 0.50, p = 0.005; right: r = 0.42, p = 0.023) at L4-5 and the NRS scores. Additionally, the shear modulus of the MF (r = 0.50, p = 0.005) on the left side of L1-2 showed similar correlations. Strong correlations were observed between the shear modulus of the bilateral TLF (left: r = 0.60, p < 0.001; right: r = 0.58, p < 0.001) at L4-5 and the ODI scores. Moderate correlations were observed between the shear modulus of the right TLF (r = 0.43, p = 0.017), ES (r = 0.38, p = 0.037), and MF (r = 0.44, p = 0.015) at L1-2, as well as the bilateral MF (left: r = 0.46, p = 0.011; right: r = 0.45, p = 0.012) at L4-5, and the ODI scores. No significant correlations were found at other measurement sites.

Conclusion

In patients with CNLBP, the stiffness of the lumbar fascia and muscles is generally higher than in individuals without LBP. However, this increase is not uniform across all lumbar regions, with the most significant changes observed in the L4-5 segments. In addition, higher stiffness may be associated with pain and dysfunction, primarily manifested in the TLF.
Using an improved U-Net++ with a T-Max-Avg-Pooling layer as a rapid approach for concrete crack detection
Authors: Sarhadi Ali , Ravanshadnia Mehdi , Monirabbasi Armin , Ghanbari Milad
Published: November 2024

The monitoring of concrete structures has advanced remarkably with the aid of deep learning technologies. Since concrete is multi-purpose and low-cost, it is extensively used for construction purposes. Concrete is very enduring. Nevertheless, it tends to crack which endangers the integrity of the structure and results in complications. The current study offers a new image segmentation approach for detecting cracks in concrete by making use of an optimized U-Net++ architecture. The proposed model gives the features of the T-Max-Avg Pooling layer which effectively combines the advantages of traditional max and average pooling using a learnable parameter to balance feature extraction dynamically. This innovation both improves the output accuracy and processing speed and captures the fine details. In addition, it mitigates noise and transcends the limitations of conventional pooling methods. Moreover, using learnable pruning and shortening skip connections in U-Net++ reduce redundant computations, making the model faster without compromising accuracy. In comparison with other models like Mask R-CNN and VGG-U-Net, the proposed model had considerably faster inference times (21.01 ms per image) and fewer computational requirements (40G FLOPs), making it very suitable for real-time monitoring applications. The DeepCrack and Concrete Pavement Crack datasets were employed to assess the model thoroughly which yielded an MIoU score of 82.1%, an F1 score of 90.12%, a Dice loss score of 93.7%, and an overall accuracy of 97.65%. According to the results, the enhanced U-Net++ with T-Max-Avg Pooling provided a balanced trade-off between segmentation accuracy and computational efficiency. This indicates its considerable potential for automated real-time crack detection in concrete structures by employing resource-constrained environments including drones and mobile platforms.
Enhancement of surfactant performance via titanium dioxide nanoparticles: implication for oil recovery in sandstone
Authors: Hidayat Miftah , Megayanti Rima , Cahyaningtyas Ndaru , Sanmurjana Mahruri , Yahya Zeta Nur Muhammad , Adityawarman  , Siagian Utjok W. R. , Marhaendrajana Taufan
Published: November 2024

The application of titanium dioxide nanoparticles in the petroleum research area has received ample attention in recent years owing to its impact on wettability-altering agents. Further, employing a surfactant injection to improve oil production in sandstone formations on an industrial scale has become an alternative solution, particularly for mature fields. However, the existing literature on the combination of alkyl ethoxy carboxylate (AEC) surfactant with titanium dioxide nanoparticles on the application of enhanced oil recovery in sandstone formations remains underreported. This study explores the impact of combining AEC surfactant with titanium dioxide nanoparticles on recovering trapped oil in sandstone by examining the interfacial tension, contact angle, zeta potential, and core flooding with various concentrations of added titanium dioxide nanoparticles (0, 0.01, 0.025, and 0.05 wt%) on AEC surfactant. Although the addition of 0.05 wt% TiO2 to AEC surfactant can significantly reduce the interfacial tension to the lowest value of 5.85 × 10−5 mN/m, our results show that the highest oil recovery in Berea sandstone (59.52% recovery factor) is achieved at the concentration of 0.025 wt% added TiO2 to AEC surfactant. We find that the stability of TiO2 nanoparticles on AEC surfactant plays a significant role in getting maximum oil recovery. These important findings from this study contribute to improving our understanding on the application of TiO2 combined with AEC surfactant to achieve more efficient and sustainable enhanced oil recovery in sandstone.
Synthesis of chitosan nanoparticles (CSNP): effect of CH-CH-TPP ratio on size and stability of NPs
Authors: Des Bouillons-Gamboa Rosvin E. , Montes de Oca Gabriela , Baudrit Jose Roberto Vega , Ríos Duarte Liz Carolina , Lopretti Mary , Rentería Urquiza Maite , Zúñiga-Umaña Juan Miguel , Barreiro Filomena , Vázquez Patricia
Published: November 2024

In the face of a pressing global issue-the escalating threat of antibiotic resistance-the development of new antimicrobial agents is urgent. Nanotechnology, with its innovative approach, emerges as a promising solution to enhance the efficacy of these agents and combat the challenge of microbial resistance. Chitosan nanoparticles (CSNPs) stand out in biomedical applications, particularly in the controlled release of antibiotics, with their unique properties such as biocompatibility, stability, biodegradability, non-toxicity, and simple synthesis processes suitable for sensitive molecules. This study synthesized CSNPs using the ionotropic gelation method, with tripolyphosphate (TPP) as the crosslinking agent. Various CS: TPP ratios (6:1, 5:1, 4:1, 3:1, 2:1) were tested, and the resulting nanoparticles were evaluated using dynamic light scattering (DLS). The CS: TPP ratio of 4:1, with an average hydrodynamic diameter (DHP) of (195 ± 10) nm and a zeta potential of (51 ± 1) mV, was identified as the most suitable for further analysis. The characterization of NPs by Transmission Electron Microscope (TEM) and atomic force microscopy (AFM) revealed diameters of (65 ± 14) nm and (102 ± 18) nm, respectively. Notably, CSNPs exhibited significant aggregation during centrifugation and lyophilization, leading to diameter increases of up to 285% as measured by AFM. The antibacterial activity of CSNPs against Staphylococcus aureus and Escherichia coli was assessed using the resazurin assay. It was found that CSNPs not subjected to centrifugation, freezing, and lyophilization retained their antimicrobial activity. In contrast, those that underwent these processes lost their efficacy, likely due to aggregation and destabilization of the system. This study presents a straightforward and effective protocol for encapsulating sensitive active agents and synthesizing chitosan nanoparticles, a potential system with significant implications in the fight against antibiotic resistance.
Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases
Authors: Zhao Yu , Zhang Wenhui , Zhang Xin
Published: November 2024

Metagenomic next-generation sequencing (mNGS) is a transformative approach in the diagnosis of infectious diseases, utilizing unbiased high-throughput sequencing to directly detect and characterize microbial genomes from clinical samples. This review comprehensively outlines the fundamental principles, sequencing workflow, and platforms utilized in mNGS technology. The methodological backbone involves shotgun sequencing of total nucleic acids extracted from diverse sample types, enabling simultaneous detection of bacteria, viruses, fungi, and parasites without prior knowledge of the infectious agent. Key advantages of mNGS include its capability to identify rare, novel, or unculturable pathogens, providing a more comprehensive view of microbial communities compared to traditional culture-based methods. Despite these strengths, challenges such as data analysis complexity, high cost, and the need for optimized sample preparation protocols remain significant hurdles. The application of mNGS across various systemic infections highlights its clinical utility. Case studies discussed in this review illustrate its efficacy in diagnosing respiratory tract infections, bloodstream infections, central nervous system infections, gastrointestinal infections, and others. By rapidly identifying pathogens and their genomic characteristics, mNGS facilitates timely and targeted therapeutic interventions, thereby improving patient outcomes and infection control measures. Looking ahead, the future of mNGS in infectious disease diagnostics appears promising. Advances in bioinformatics tools and sequencing technologies are anticipated to streamline data analysis, enhance sensitivity and specificity, and reduce turnaround times. Integration with clinical decision support systems promises to further optimize mNGS utilization in routine clinical practice. In conclusion, mNGS represents a paradigm shift in the field of infectious disease diagnostics, offering unparalleled insights into microbial diversity and pathogenesis. While challenges persist, ongoing technological advancements hold immense potential to consolidate mNGS as a pivotal tool in the armamentarium of modern medicine, empowering clinicians with precise, rapid, and comprehensive pathogen detection capabilities.
Periodontal bacteria influence systemic diseases through the gut microbiota
Authors: Xi Mengying , Ruan Qijun , Zhong Sulan , Li Jiatong , Qi Weijuan , Xie Congman , Wang Xiaoyan , Abuduxiku Nuerbiya , Ni Jia
Published: November 2024

Many systemic diseases, including Alzheimer disease (AD), diabetes mellitus (DM) and cardiovascular disease, are associated with microbiota dysbiosis. The oral and intestinal microbiota are directly connected anatomically, and communicate with each other through the oral-gut microbiome axis to establish and maintain host microbial homeostasis. In addition to directly, periodontal bacteria may also be indirectly involved in the regulation of systemic health and disease through the disturbed gut. This paper provides evidence for the role of periodontal bacteria in systemic diseases via the oral-gut axis and the far-reaching implications of maintaining periodontal health in reducing the risk of many intestinal and parenteral diseases. This may provide insight into the underlying pathogenesis of many systemic diseases and the search for new preventive and therapeutic strategies.
Marine climate indicators in the Adriatic Sea
Authors: da Costa Vladimir Santos , Alessandri Jacopo , Verri Giorgia , Mentaschi Lorenzo , Guerra Roberta , Pinardi Nadia
Published: November 2024

This research seeks to categorize and ascertain the primary marine climatic indicators within the complex Adriatic Sea area. Employing subregional climate downscaling models with resolution on the scale of a few kilometers, incorporating atmospheric, oceanic, and hydrological coupled models, the study scrutinizes historical baseline simulations (from 1992 to 2011) and future projections (from 2031 to 2050) under the RCP8.5 scenario. The chosen climate indicators are related to Sea Surface Temperature (SST), Marine Heat Waves (MHWs), Brunt–Väisälä frequency, Sea Level Rise (SLR), and Ocean Heat Content (OHC). The main results show the positive trend in SST and its correlation to circulation structures. It is noticeable that the historical period reveals a greater trend compared to the projection period, being 0.04°C/year and 0.022°C/year, respectively. The OHC shows the expected positive trend with a maximum increase in the southern Adriatic Gyre. The stability of the water column, as identified by the Brunt–Väisälä frequency values, is decreased in the shallow northern Adriatic due to the river discharge decrease while it is increased in the mid-depth water column of the central and southern regions. The number and amplitude of MHW increases especially if referenced to the historical period and finally the rate of total sea level rise shows a consistent decrease in the projection period due to compensating effects between warming and salting and the changing water budget.
Multimodal Fake News Detection with Contrastive Learning and Optimal Transport
Authors: Shen Xiaorong , Huang Maowei , Hu Zheng , Cai Shimin , Zhou Tao
Published: November 2024

Introduction

The proliferation of social media platforms has facilitated the spread of fake news, posing significant risks to public perception and societal stability. Existing methods for multimodal fake news detection have made important progress in combining textual and visual information but still face challenges in effectively aligning and merging these different types of data. These challenges often result in incomplete or inaccurate feature representations, thereby limiting overall performance.

Methods

To address these limitations, we propose a novel framework named MCOT (Multimodal Fake News Detection with Contrastive Learning and Optimal Transport). MCOT integrates textual and visual information through three key components: cross-modal attention mechanism, contrastive learning, and optimal transport. Specifically, we first use cross-modal attention mechanism to enhance the interaction between text and image features. Then, we employ contrastive learning to align related embeddings while distinguishing unrelated pairs, and we apply optimal transport to refine the alignment of feature distributions across modalities.

Results

This integrated approach results in more precise and robust feature representations, thus enhancing detection accuracy. Experimental results on two public datasets demonstrate that the proposed MCOT outperforms state-of-the-art methods.

Discussion

Our future work will focus on improving its generalization and expanding its capabilities to additional modalities.
Early growth response-1, a dynamic conduit in cardiovascular disease
Authors: Khachigian Levon M.
Published: November 2024

The transcription factor, early growth response-1 (Egr-1) is the product of a prototypic immediate-early gene that plays an integral role in the pathogenesis of multiple cardiovascular diseases. Egr-1 has been linked with atherogenesis, myocardial ischemia-reperfusion injury, cardiac fibrosis and heart failure. Egr-1 expression is triggered by a host of factors including cytokines, hormones, growth factors, hyperglycaemia, biomechanical forces and oxygen deprivation. Egr-1 is a molecular conduit that links changes in the cellular environment with the inducible expression of genes whose products play a causative role in this inflammatory disease. It is rapidly synthesised, undergoes post-translational modification, interacts with a range of cofactors and drives gene expression. Studies in Egr-1 deficient mice, animal models using DNAzymes, RNA interference, oligodeoxynucleotide decoys, antisense oligonucleotides, and new insights provided by technologies such as single cell RNA sequencing, have shaped our understanding of the importance of Egr-1 in the initiation and progression of cardiovascular disease. This article describes Egr-1's role in various cardiovascular settings and discusses potential mechanisms of action. Given the range of conditions linked to Egr-1, this zinc finger protein may serve as a therapeutic target for intervention.
Functional block in the initiation and maintenance of common flutter: detailed electrophysiological study and electro-anatomical mapping
Authors: Arnaud Marine , Sacristan Benjamin , Hocini Meleze , Jais Pierre , Haissaguerre Michel , Duchateau Josselin
Published: November 2024

Introduction

The precise pathophysiology of common atrial flutter remains imperfectly known. The mechanisms of arrhythmia initiation and the role of areas of slow conducting myocardium and functional block are still debated topics.

Methods

We conducted a detailed electrophysiological study of a patient to illustrate and refine these concepts. Prior to CTI ablation, electrophysiological study and electro-anatomical mapping were performed, focusing on initiation and maintenance mechanisms of the arrhythmia.

Results

The initiation of common atrial flutter takes place on the septal aspect of the cavo-tricuspid isthmus where functional unidirectional conduction block occurs. The direction of activation is therefore frequently counter-clockwise, and the arrhythmia stabilizes around the vena cavas and sinus venosus/crista terminalis region. No conduction slowing is present.

Conclusions

Common atrial flutter initiates when functional unidirectional conduction block occurs on the septal cavotricuspid isthmus. Its rotation is limited by anatomical and functional boundaries.
Camera-view supervision for bird's-eye-view semantic segmentation
Authors: Yang Bowen , Yu LinLin , Chen Feng
Published: November 2024

Bird's-eye-view Semantic Segmentation (BEVSS) is a powerful and crucial component of planning and control systems in many autonomous vehicles. Current methods rely on end-to-end learning to train models, leading to indirectly supervised and inaccurate camera-to-BEV projections. We propose a novel method of supervising feature extraction with camera-view depth and segmentation information, which improves the quality of feature extraction and projection in the BEVSS pipeline. Our model, evaluated on the nuScenes dataset, shows a 3.8% improvement in Intersection-over-Union (IoU) for vehicle segmentation and a 30-fold reduction in depth error compared to baselines, while maintaining competitive inference times of 32 FPS. This method offers more accurate and reliable BEVSS for real-time autonomous driving systems. The codes and implementation details and code can be found at https://github.com/bluffish/sucam.
Digital media in early childhood: risk factors for online harm and psychosocial correlates
Authors: Gath Megan , Swit Cara
Published: November 2024

Introduction

Early and middle childhood are times of rapid development, and critical periods for laying the foundations of life-long trajectories of socioemotional well-being. High levels of screen media use are of growing concern to parents, health professionals, and researchers, given the increasing body of research demonstrating detrimental impacts of excessive screen use in young children. One particular consequence is the risk that children encounter online content or experiences that are upsetting or distressing, including exposure to inappropriate or adult content, cyberbullying, and interactions with strangers that they don't know.

Methods

This research examined experiences of online harm reported in a sample of 8-year-old children, with a focus on identifying risk factors and psychosocial correlates of online harm. Data for this study were collected from children and their mothers as part of the prospective longitudinal Growing Up in New Zealand (GUiNZ) study (n = 4,920 children with data at age 8). Children were assessed at 4.5-years-old and 8-years-old.

Results

The findings of this research indicate that approximately a quarter of New Zealand children have experienced online harm (that is, have encountered online content that worried, upset, or bothered them) by the age of 8. Our analysis indicates that children with behavioral difficulties are at greater risk of online harm, as are children with more personal devices. Experiences of online harm were found to be negatively associated with child self-worth and positively associated with depressive symptoms.

Discussion

Findings highlighting the critical importance of considering online harm as a contributing factor to child and youth well-being and mental health in our media-saturated world. Our results also point to practical solutions for parents, such as limiting the number of personal media devices that children have in early and middle childhood.
Titanite as an indicator of granite fertility and gold mineralization in the Xiaoqinling gold province, North China Craton
Authors: Wei Quan , Li Lin , Li Sheng-Rong , Santosh M. , Alam Masroor , Chen Zhen-Yu , Li Min-Gang , Chen Xiao-Dan , Wen Zi-Hao , Liu Jia-Wei
Published: November 2024

The Xiaoqinling gold province, located in the southern margin of the North China Craton (NCC), is the second largest gold-enriched region in China. In this region, the Mesozoic Huashan (HS) and Wenyu (WY) plutons are the major magmatic intrusions coeval with gold mineralization, although they show contrasting characteristics in the distribution of gold. In this study, we use geochemical features of titanite determined by LA-ICP-MS and EPMA analyses and elemental mapping to decipher the mechanisms that led to the difference in gold enrichment related to the two plutons. Titanite from the Wenyu granitic pluton exhibits significantly higher (La/Sm)N, (La/Yb)N, ΣLREE/ΣHREE ratios, and ΣREE concentration and slightly higher (Gd/Yb)N values than those of the Huashan Pluton, suggesting that the Wenyu pluton might have experienced more complex magmatic evolution, widespread hydrothermal alteration, and higher silica activity in the melt than the Huashan pluton. The titanite grains from the Huashan pluton show higher (Nb/Ta)N and (Lu/Hf)N values and significantly lower Zr concentration than those of the Wenyu pluton. The titanite grains from the Wenyu pluton show higher vanadium and gallium concentrations and Fe/Al ratio than those of the Huashan pluton, indicating comparatively higher fo2. Furthermore, the titanite grains from Wenyu pluton indicate higher water content in the magma. In addition, magma mingling and magmatic hydrothermal fluids derived from the crust/mantle are critical sources for ore-forming materials. These results suggest that the Wenyu pluton is more conducive to gold migration and enrichment than the Huashan pluton.
Late Quaternary fluvial terrace characteristics and ages of the Pamir‒Tian Shan convergence zone: indications of regional climate change and tectonic uplift
Authors: Deng Jinhua , Li Tao , Feng Jiahui , Qian Li
Published: November 2024

The Pamir‒Tian Shan collision zone, located at the northwestern edge of the Himalaya‒Qingzang orogenic belt, provides a natural laboratory for investigating the development of fluvial terraces controlled by regional climate change and tectonic uplift. In this study, we conducted geomorphological mapping and terrestrial cosmogenic nuclide 10Be dating of fluvial terraces in the Wuheshalu syncline, within the Pamir‒Tian Shan collision zone. Four major fluvial terraces were identified in the Wuheshalu syncline, with ages of approximately 187, 141, 90, and 19 ka, respectively. These terraces were abandoned during three glacial‒interglacial transition periods (Marine Isotope Stage 6/5 (MIS6/5), cold-to-warm transition period of MIS5, and end of MIS2 or MIS2/1) and one interglacial‒glacial period (MIS7/6), and exhibit a strong correlation with regional climate change. Tectonic uplift contributed only one-third of the observed fluvial incision. These results suggest that river incision and terrace formation in the Pamir‒Tian Shan collision zone are primarily driven by periodic fluctuations in climate, with a lesser contribution from tectonic uplift.
Functional analysis of fibroblasts and macrophages in head and neck paragangliomas
Authors: Baruah Paramita , Marshall Jennifer L. , Nefla Meriam , Pucino Valentina , Adams Holly , Turner Jason D. , Gilbert Sebastian , Powell Emily , Neag Georgiana , Monksfield Peter , Irving Richard M. , Croft Adam P. , Dumitriu Ingrid E. , Buckley Christopher D.
Published: November 2024

Background and aim

Head and neck paragangliomas (HNPGN) are tumours that carry significant morbidity The role of the stroma in the pathogenesis of HNPGN is not completely understood. This study explores the profile of fibroblasts and macrophages in HNPGN.

Methods

Ten patients undergoing HNPGN surgery were recruited. CD68 and CD163 immunohistochemistry was performed for macrophage analysis; CD90 and podoplanin (PDPN) expression was examined to identify fibroblasts. RT-qPCR was performed on HNPGN tissue for macrophage- and fibroblast-associated molecules. Fibroblast cultures were established from HNPGN were analysed by RT-qPCR and flowcytometry. Confocal microscopy for MCT1 and MCT4 was performed in HNPGN.

Results

CD68 and CD163 expressing macrophages were noted in HNPGN. CD90 and PDPN expressing cells were present in HNPGN. RT-qPCR analysis showed expression of phenotypic and functional macrophage- and fibroblast-associated molecules in HNPGN. RT-qPCR analysis of fibroblasts cultured from HNPGN confirmed the expression of several molecules including PDPN at comparable levels to healthy tissue fibroblasts. Expression of FAP, MCT-1, insulin receptor (CD220) and insulin growth factor receptor-2 (CD222) was noted on HNPGN derived fibroblasts on flowcytometry. MCT1 and MCT4 were expressed in HNPGN tumour cells and stromal macrophages in-situ.

Conclusion

Fibroblasts and macrophages are present in the HNPGN tumour microenvironment, and several macrophage and fibroblast functional markers are expressed in HNPGN. Macrophages in HNPGN tissue express metabolic markers MCT1 and MCT4. Further analysis of the fibroblast and macrophage function in HNPGN will improve our understanding of their potential roles in tumour pathogenesis.
Improving corporate profitability from the inside out with environmental performance: an empirical analysis based on Chinese listed company data
Authors: Hu Jing , Zhao Yanyun
Published: November 2024

This study uses data from listed companies in China from 2008 to 2021, constructs a comprehensive evaluation of corporate environmental performance from the perspective of internal efficiency and externalities based on the text analysis method, and analyzes the impact and mechanism of environmental performance on profitability. In addition, we find that improving environmental performance can significantly increase profitability, especially if the company tackles pollution at the source. Furthermore, the impact of environmental performance on profitability tends to be driven by external markets. Obtaining environmental certifications, awards, and green patents can provide positive feedback and significantly increase profitability. In addition, the mechanism analysis shows that environmental performance affects firm profitability by improving green technology innovation, reducing financing constraints and increasing operating income. Finally, the results of the heterogeneity analysis show that the profitability-enhancing effect of environmental performance is stronger in non-state-owned firms, in clean industries firms and in regions with carbon emission trading.
Gut Microbiota’s role in lipoma development: evidence from mendelian randomization
Authors: Li Yuxin , Chen Jiahao , Yao Hang , Xu Xiaogang , Zheng Xianglong , Wang Yu , Wang Wanchun
Published: November 2024

Background

Lipoma, a benign tumor derived from mesenchymal tissue, significantly affects patients’ physical and psychological wellbeing. Increasing evidence points to a strong link between the gut microbiome (GM) and lipoma incidence. This study utilizes Mendelian Randomization (MR) to assess the potential causal relationships between the GM and lipoma development.

Methods

We conducted a two-sample MR analysis using genome-wide association study (GWAS) data from MiBioGen and FinnGen to explore the causal relationship between GM and lipoma. The GM dataset included 18,340 participants with 14,587 single nucleotide polymorphisms (SNPs), while the lipoma dataset comprised 412,181 participants with 21,306,349 SNPs. We employed 5 MR methods: Inverse Variance Weighted (IVW), Weighted Median, Simple Mode, MR-Egger, and Weighted Mode. Additional assessments included Cochran’s Q test for result heterogeneity, PRESSO analysis for horizontal pleiotropy, and sensitivity analyses through scatter plots, leave-one-out analyses, funnel plots, and forest plots.

Results

The IVW method identified 18 gene predictors trans-genus associated with lipoma risk. Protective effects against benign lipoma (BL) were observed in the Eubacterium rectale group, Desulfovibrio, Ruminococcus1, Clostridium sensu stricto1, and Lachnospiraceae UCG001; conversely, Lachnospiraceae UCG008 was linked to increased BL risk. Desulfovibrio provided protection against TS-BL; however, the Family XIII AD3011 group, Eubacterium coprostanoligenes group, Lachnospiraceae NK4A136 group, and Parasutterella were associated with an increased TS-BL risk. The Clostridium innocuum group, Eubacterium rectale group, Anaerotruncus, Ruminiclostridium6, and Lachnospiraceae UCG001 offered protection against LS-BL, while Lachnospiraceae UCG008 was linked to an increased LS-BL risk. The Eubacterium brachy group, Odoribacter, Butyricimonas, Subdoligranulum, and Clostridium sensu stricto1 were protective against HFNS-BL; Ruminococcaceae UCG005 was associated with an increased HFNS-BL risk.

Conclusion

Compared to malignant tumors, research on lipomas has been relatively limited. This study, through MR analysis, provided new evidence of a causal relationship between specific GM and the development of lipomas. Certain gut bacterial species may act as protective or harmful factors in lipoma formation, offering new avenues for future treatment strategies. However, additional research is required to unravel the complexity of how GM influences the pathogenesis of lipomas.
Global trends in tertiary lymphoid structures: a bibliometric analysis from 2014 to 2023
Authors: Bao Yiwen , Mo Zeming , Wang Shuang , Long Jinhua , Zhang Honghong , Xu Yujun , Jiang Honglian , Qian Tianbao , Zeng Zhu
Published: November 2024

Aim and background

Tertiary lymphoid structures (TLS) are increasingly recognized for their role in immunity. Despite growing interest, a systematic bibliometric analysis of TLS-related research has been lacking. To provide a comprehensive overview of current research trends and hotspots, we conducted a bibliometric analysis using data from the Web of Science Core Collection.

Methods

We retrieved TLS-related publications from the Science Citation Index Expanded within the Web of Science Core Collection from January 2014 to December 2023. Co-occurrence analysis with “VOSviewer” identified current status and research hotspots, while “CiteSpace” was used for co-citation analysis to assess knowledge evolution and bursts. Thematic evolution was explored using bibliometrics to identify emerging keyword trends. Additionally, we examined country/region, institutional, and author contributions and collaborations. Tables were created using Microsoft Word.

Results

A total of 785 publications were analyzed, showing a continuous growth trend from 2017 to 2023, indicating escalating interest in TLS among researchers. Leading countries in TLS research were China (231 publications), the United States (212 publications), and France (89 publications). The most productive institution and author were the “Institut national de la santé et de la recherche médicale” (70 publications) and Catherine Sautes-Fridman (21 publications), respectively. Key topics included TLS, B cells, and immunotherapy. Recent research has focused on mechanisms linking TLS with cancers, such as immunotherapy, tumor microenvironment, tumor-infiltrating lymphocytes, prognosis, and immune checkpoint inhibitors, highlighting an expanding area of study. Additionally, TLS’ potential as a biomarker for predicting immunotherapy efficacy across different cancer types remains a burgeoning research direction.

Conclusions

This study provides a comprehensive analysis of global TLS-related publications, revealing key literature metrics and identifying influential articles and emerging research concerns. These findings contribute valuable insights into the role of TLS in immunotherapy and suggest future directions for this dynamic field.
Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study
Authors: del Pino Molina Lucía , Reche Yebra Keren , Soto Serrano Yolanda , Clemente Bernal Álvaro , Avendaño-Monje Carmen L. , Ocejo-Vinyals J. Gonzalo , Rodríguez Pena Rebeca , López Granados Eduardo
Published: November 2024

Background

The use of next-generation sequencing in inborn errors of immunity (IEI) has considerably increased the identification of novel gene variants, many of which are identified in patients without the described clinical phenotype or with variants of uncertain pathogenic significance in previously described genes. Properly designed functional and cellular assays, many necessarily accomplished by research-based laboratories, reveal the pathogenic consequences of the gene variants and contribute to diagnosis. Activated PI3Kδ syndrome (APDS) is a rare disease that can be divided into APDS1, caused by gain of function (GOF) mutations in PIK3CD gene, and APDS2, with loss of function (LOF) variants in the PIK3R1 gene. Both entities present hyperactivation of the PI3K pathway, which can be analyzed through Akt and S6 phosphorylation status.

Methods

Our objective was to perform an accurate, robust, and reproducible functional assay to analyze the phosphorylation status of proteins in the PI3K-Akt-S6 pathway by flow cytometry, to contribute to diagnosis, to monitor treatments, and to establish intra-assay standardization.

Results

We illustrate the robustness and reproducibility of our experimental procedure in patients with APDS who had high Akt and/or S6 phosphorylation levels at baseline, and after anti-IgM stimulation in B cells. We show the relevance of an appropriate cohort of samples from healthy donors, processed within the same conditions as the suspected samples, in particular the time frame for sample processing once blood is collected.

Discussion

We highlight the importance of B cell stimulation through B cell receptor signaling, which is highly recommended, especially for samples that would be processed more than 24 hours after blood extraction. Also, having a defined experimental procedure is important, including the cytometer setup, which allows cytometer reproducibility for a period of time, enabling the comparison of a sample at different times.
Case report: Infection-associated HPS during pregnancy cured by HLH-94 protocol with induction therapy of ruxolitinib
Authors: Cen Tianqi , Xuan Weixia , Huang Shaohui , Wang Ziqi , Shen Lijun , Zhang Moyuan , Fang Jinzhou , Yang Shenying , Zhang Xiaoju
Published: November 2024

Hemophagocytic syndrome (HPS) is a rapidly progressive and highly fatal disease, and is even more complex when it occurs during pregnancy. Currently, the HLH-94 protocol is commonly used for treatment for HPS, with ruxolitinib being mostly used for salvage therapy. Here, we report a pregnant woman who presented with fever, thrombocytopenia, splenomegaly, and subsequently developed into severe pneumonia and multiple organ dysfunction(MODS). The patient was diagnosed as HPS based on clinical manifestations, laboratory indexes, and hemophagocytosis observed in bone marrow aspirate smear. After receiving ruxolitinib as induction therapy combined with HLH-94 protocol, the patient significantly improved and eventually cured.
The role of histone post-translational modifications in cancer and cancer immunity: functions, mechanisms and therapeutic implications
Authors: Duan Xiaohong , Xing Zhiyao , Qiao Lu , Qin Shan , Zhao Xuejing , Gong Yanhua , Li Xueren
Published: November 2024

Histones play crucial roles in both promoting and repressing gene expression, primarily regulated through post-translational modifications (PTMs) at specific amino acid residues. Histone PTMs, including methylation, acetylation, ubiquitination, phosphorylation, lactylation, butyrylation, and propionylation, act as important epigenetic markers. These modifications influence not only chromatin compaction but also gene expression. Their importance extends to the treatment and prevention of various human diseases, particularly cancer, due to their involvement in key cellular processes. Abnormal histone modifications and the enzymes responsible for these alterations often serve as critical drivers in tumor cell proliferation, invasion, apoptosis, and stemness. This review introduces key histone PTMs and the enzymes responsible for these modifications, examining their impact on tumorigenesis and cancer progression. Furthermore, it explores therapeutic strategies targeting histone PTMs and offers recommendations for identifying new potential therapeutic targets.
Seaweed extracts: enhancing plant resilience to biotic and abiotic stresses
Authors: Kumar Gagan , Nanda Satyabrata , Singh Sushil Kumar , Kumar Sanjeet , Singh Divya , Singh Bansh Narayan , Mukherjee Arpan
Published: November 2024

Seaweeds are a natural marine resource containing many bioactive compounds such as amino acids, lipids, carbohydrates, proteins, phytohormones, and antimicrobial compounds. Since ancient times, seaweeds have been used in various sectors, including medicine, food, and the cosmetic industry. Currently, seaweeds are a promising alternative to reduce the application of harmful chemicals in agriculture. Seaweed and its derived products have been utilized for plant growth promotion, immunity enhancement, and the reduction of biotic and abiotic stresses. In the current global scenario, synthetic fertilizers and chemical pesticides are commonly used to increase agricultural crop production to meet the growing demands of the world population. However, these chemicals pose significant threats to the health of people, livestock, plants, soil, and the entire environment. In contrast, seaweed-based products are emerging as a newer option for stress mitigation and reduction, offering an alternative to synthetic chemicals. This article explains the use of seaweed extracts to increase the tolerance of plants to biotic and abiotic stresses. We also address the functions of various bioactive compounds present in seaweed extracts and the mechanisms by which they promote plant growth and induce defense against different stresses.
Ocean acidification enhances the tolerance of dinoflagellate Prorocentrum donghaiense to nanoplastic-induced oxidative stress by modulating photosynthetic performance
Authors: Zhu Yue , Lin Qingming , Yang Yao , Xia Yanmei , Cai Huidi , Feng Xucong , Gonçalves Rodrigo J. , Guan Wanchun
Published: November 2024

Introduction

The impact of ocean acidification (OA) and nanoplastics (NPs) on harmful algal blooms (HAB) has emerged as a major global concern. However, the combined effects of OA and NPs on the HAB species are poorly understood.

Methods

In this study, dinoflagellate Prorocentrum donghaiense, a typical HAB species, was exposed to varying concentrations of NPs (108.15 ± 8.52 nm) (0, 5, 10, and 15 mg L−1) and CO2 (low CO2: 417 ppm, pH: 8.00 and high CO2: 1045 ppm, pH: 7.73) for seven days to investigate the combined effects of OA and NPs.

Results and discussion

The findings revealed that NPs inhibited the growth of P. donghaiense by inducing oxidative stress, as indicated by elevated malondialdehyde (MDA) content and decreased carotenoid/chlorophyll-a ratio, even though photochemical efficiency (φP0, ψ0, and φE0), rETRmax and α were enhanced in response to NPs stress. However, OA promoted the growth and alleviated the adverse effects of NPs on P. donghaiense by increasing photochemical efficiency (φP0, ψ0, and φE0) and energy flux (RC/CS0, TR0/CS0, ET0/CS0) and enhancing the antioxidant ability (increased superoxide dismutase, and decreased MDA). P. donghaiense showed enhanced tolerance to NPs under simulated OA conditions. These findings enhance our knowledge of the HAB species response to NPs pollution under future OA scenarios.
Risk assessment of abnormal pressure zones with a focus on shallow gas pockets based on surface gas logging data and statistical relationships
Authors: Hazbeh Omid , Rajabi Meysam , Esmaeili Korani Sahar , Ghorbani Hamzeh , Soleimani Bahman , Gajbhiye Rahul
Published: November 2024

The assessment of alterations in hydrocarbon components preserved in reservoirs is important for oil field management and drilling procedures. Drilling fluid analysis yields valuable insights into the reservoir fluid. This study focuses on identifying and quantifying shallow gas basins in the Azadegan oil field. Risk assessment of abnormal pressure zones with a focus on shallow gas pockets based on surface gas logging data and statistical relationships is also a key aspect of this research. Shifts in the C1 gas ratio to other gas components signify a notable peak in the Asmari Formation. Consistency among various wetness (Wh), balance (Bh), and character (Ch) indices suggests that the Aghajari and Gachsaran formations potentially harbor gas and contain some heavy or residual oil with limited production potential. However, the Asmari Formation shows the potential for natural gas production, while the Gurpi Formation has the potential for wet gas or condensate. Pixler parameters were computed and plotted to affirm the results of other parameters. The investigation of stratigraphic columns for component changes and lithology reveals that lithology plays a pivotal role in preserving valuable fluids. The upper section of the Aghajari Formation, primarily composed of shale-evaporite horizons, exhibits lower frequency compared to its lower part. The Gachsaran Formation displays an exceptional ability to preserve components in anhydrite sections. Although Asmari has a high accretion ratio, the Chilean calcareous sandstone horizons are more prominent in the upper part of the Gurpi Formation. The structural model includes humpback microstructures as controls for shallow gas pockets and component accumulation. Evaporite and shale layers significantly influence the movement of these components across different horizons. The 3D model illustrates migration from the Gurpi Formation to the Aghajari Formation, as both formations serve as sources due to the propagation paths leading to the Pabdeh and Gurpi Formations.
The mystery of abdominal snow-grip sensation: emphysematous cystitis complicated with bladder gangrene and rupture—case report and literature review
Authors: Zhou Yiwen , Cai Yuxi , Yang Yi , Xu Chenyang , Xiang Jianbin , Fang Zujun , Mao Shanhua
Published: November 2024

Emphysematous cystitis (EC) refers to a rare form of complicated urinary tract infection (UTI), which is typically observed in elderly females with severe diabetes mellitus. EC was triggered by bacteria, producing gas filled cysts in the bladder wall and lumen, the most common of which are Escherichia coli and Klebsiella pneumoniae. Since EC could be potentially life-threatening, severe form of EC (like septic shock) can cause gangrenous cystitis (GC). This case report describes an 85-year-old female patient with type 2 diabetes mellitus (DM) who presented with EC and bladder gangrene due to the growth of Candida glabrata in urine. Our case describes patient’s progression in detail, ranging from the initial clinical manifestations to specific management actions, which may provide new insights and ideas for the diagnosis and treatment of such disease.