bluuebunny/crossref_metadata_2025 huggingface dataset:
Antecedents and Contextual Factors Affecting Occupational Turnover among Registered Nurses in Public Hospitals in Hong Kong: A Qualitative Descriptive Study
Authors: Hung Maria S. Y. , Lam Stanley K. K.
Published: May 2020

Global increases in both population size and ageing have led to a drastic expansion in the demand for healthcare services. The shortage of nursing workforce capacity continues, posing immense challenges for the global healthcare system. We aimed to identify the antecedents and contextual factors that contribute to the decisions of occupational turnover from the clinical duties of registered nurses in public hospitals in Hong Kong. A qualitative descriptive design was used in this study. A total of 18 registered nurses who had resigned from public hospitals in Hong Kong and changed their occupations were recruited via convenience and snowball sampling methods. Data were collected through individual, semi-structured, and face-to-face interviews and were analyzed according to the content analysis approach. The antecedents and contextual factors that contributed to the registered nurses’ decisions regarding occupational turnover were identified from the collected data. These factors were classified into three overarching categories: (1) job dissatisfaction due to a tense work environment, (2) low motivation due to limited career opportunities, and (3) inadequate communication due to ineffective leadership. The identification of these antecedents and contextual factors could help healthcare service providers to develop strategies to enhance nurses’ commitment and engagement in their positions and eventually improve their retention. Based on these factors, healthcare sector policy makers could consider incorporating appropriate strategies into healthcare system policy.
Strategies for Attention to Diversity: Perceptions of Secondary School Teaching Staff
Authors: Goig Martínez Rosa , Martínez Sánchez Isabel , González González Daniel , García Llamas José Luis
Published: May 2020

(1) Background: Attention to diversity constitutes an aspect that influences system quality and offers a perspective of the capacity of educational centres to respond to educational needs. The present study carried out an examination of the perceptions held by secondary school teachers and the level of importance conferred by them to the variables that should be integrated into plans and will influence the degree of compliance. (2) Methods: Quantitative descriptive research was performed using a survey to collect data from teachers at schools that had a Quality Management System available. (3) Results: Interaction with families is necessary to agree upon the centre’s objectives to address diversity and to define an optimisation strategy for resources in virtue of their availability within the centre. It is key to establish an appropriate teacher–student ratio to encourage compliance. (4) Conclusions: Teachers are the great pillars of quality education. Their perceptions are the route through which deficient aspects and the dimensions that must be improved when formulating these strategies can be recognised with attention to diversity.
Adaptation and Validation of the Multi-Dimensional Perceived Autonomy Support Scale for Physical Education to the Spanish Physical Exercise Context
Authors: Trigueros Ruben , Aguilar-Parra José M. , Sánchez-Iglesias Ana I. , González-Bernal Jerónimo J. , Mercader Isabel
Published: May 2020

The interaction between the teacher and the student is essential in order to encourage adherence to physical exercise or sports by young people. In this sense, the support of the autonomy of the teacher for the students has been analyzed in a one-dimensional way. Therefore, the aim of this study was to adapt and validate the Multidimensional Scale of Support for Autonomy Perceived for Physical Education the context of Spanish to physical exercise, in order to have a multidimensional scale. A total of 2329 young people from various educational centers in Andalusia (Spain) participated in the study. The factorial structure of the questionnaire was examined through an exploratory factorial analysis and two confirmatory factorial analyses. In addition, an analysis of invariability by sex and age was carried out. The results reflected that the validated questionnaire showed adequate psychometric properties, being invariable with respect to sex and age.
The Relationships between Economic Scarcity, Concrete Mindset and Risk Behavior: A Study of Nicaraguan Adolescents
Authors: Aguilar Pilar , Caballero Amparo , Sevillano Verónica , Fernández Itziar , Muñoz Dolores , Carrera Pilar
Published: May 2020

Background: Nicaragua is one of the poorest countries in Latin America, with an extremely low human development index (HDI). Fifty-two percent of the Nicaraguan population are children and adolescents under 18 years of age. Nicaraguan adolescents present several risk behaviors (such as teenage pregnancies, consumption of alcohol, tobacco, cannabis). Our study examines the links between risk behaviors, fatalism, real economic scarcity, and concrete construal level for adolescents with low and middle-low socioeconomic status in Nicaragua. Methods: Nicaraguan adolescents (N = 834) from schools located in especially vulnerable areas (low economic status) or in neighborhoods with middle-low social class completed several scales and questions to evaluate fatalism (SFC—social fatalism scale), construal level (BIF) and their past and future risk behaviors (smoking cigarettes, smoking cannabis, unsafe sex, and alcohol consumption). Results: We identified that the poorest individuals who maintained a concrete style of thinking had the highest rates of past and future risk behaviors. This vulnerable group also reported the highest levels of fatalism, i.e., negative attitudes and feelings of helplessness. Encouragingly, the adolescents who were able to maintain an abstract mindset reported healthier past and future habits and lower fatalism, even when they belonged to the lowest social status. In the middle-low economic group, the construal level was not as relevant to maintaining healthy habits, as adolescents reported similar rates of past and future risk behavior at both construal levels. Conclusions: All these results support the importance of considering construal level when studying vulnerable populations and designing risk prevention programs.
Diseases Associated with Defects in tRNA CCA Addition
Authors: Slade Angelo , Kattini Ribal , Campbell Chloe , Holcik Martin
Published: May 2020

tRNA nucleotidyl transferase 1 (TRNT1) is an essential enzyme catalyzing the addition of terminal cytosine-cytosine-adenosine (CCA) trinucleotides to all mature tRNAs, which is necessary for aminoacylation. It was recently discovered that partial loss-of-function mutations in TRNT1 are associated with various, seemingly unrelated human diseases including sideroblastic anemia with B-cell immunodeficiency, periodic fevers and developmental delay (SIFD), retinitis pigmentosa with erythrocyte microcytosis, and progressive B-cell immunodeficiency. In addition, even within the same disease, the severity and range of the symptoms vary greatly, suggesting a broad, pleiotropic impact of imparting TRNT1 function on diverse cellular systems. Here, we describe the current state of knowledge of the TRNT1 function and the phenotypes associated with mutations in TRNT1.
Bioinformatic Analysis Reveals Phosphodiesterase 4D-Interacting Protein as a Key Frontal Cortex Dementia Switch Gene
Authors: Potashkin Judith A. , Bottero Virginie , Santiago Jose A. , Quinn James P.
Published: May 2020

The mechanisms that initiate dementia are poorly understood and there are currently no treatments that can slow their progression. The identification of key genes and molecular pathways that may trigger dementia should help reveal potential therapeutic reagents. In this study, SWItch Miner software was used to identify phosphodiesterase 4D-interacting protein as a key factor that may lead to the development of Alzheimer’s disease, vascular dementia, and frontotemporal dementia. Inflammation, PI3K-AKT, and ubiquitin-mediated proteolysis were identified as the main pathways that are dysregulated in these dementias. All of these dementias are regulated by 12 shared transcription factors. Protein–chemical interaction network analysis of dementia switch genes revealed that valproic acid may be neuroprotective for these dementias. Collectively, we identified shared and unique dysregulated gene expression, pathways and regulatory factors among dementias. New key mechanisms that lead to the development of dementia were revealed and it is expected that these data will advance personalized medicine for patients.
Looking for a Treatment for the Early Stage of Alzheimer’s Disease: Preclinical Evidence with Co-Ultramicronized Palmitoylethanolamide and Luteolin
Authors: Facchinetti Roberta , Valenza Marta , Bronzuoli Maria Rosanna , Menegoni Giorgia , Ratano Patrizia , Steardo Luca , Campolongo Patrizia , Scuderi Caterina
Published: May 2020

Background: At the earliest stage of Alzheimer’s disease (AD), although patients are still asymptomatic, cerebral alterations have already been triggered. In addition to beta amyloid (Aβ) accumulation, both glial alterations and neuroinflammation have been documented at this stage. Starting treatment at this prodromal AD stage could be a valuable therapeutic strategy. AD requires long-term care; therefore, only compounds with a high safety profile can be used, such as the new formulation containing palmitoylethanolamide and luteolin (co-ultra PEALut) already approved for human use. Therefore, we investigated it in an in vivo pharmacological study that focused on the prodromal stage of AD. Methods: We tested the anti-inflammatory and neuroprotective effects of co-ultra PEALut (5 mg/Kg) administered for 14 days in rats that received once, 5 µg Aβ(1–42) into the hippocampus. Results: Glial activation and elevated levels of proinflammatory mediators were observed in Aβ-infused rats. Early administration of co-ultra PEALut prevented the Aβ-induced astrogliosis and microgliosis, the upregulation in gene expression of pro-inflammatory cytokines and enzymes, as well as the reduction of mRNA levels BDNF and GDNF. Our findings also highlight an important neuroprotective effect of co-ultra PEALut treatment, which promoted neuronal survival. Conclusions: Our results reveal the presence of cellular and molecular modifications in the prodromal stage of AD. Moreover, the data presented here demonstrate the ability of co-ultra PEALut to normalize such Aβ-induced alterations, suggesting it as a valuable therapeutic strategy.
Development and Characterization of an Allergic Asthma Rat Model for Interventional Studies
Authors: Périz Marta , Pérez-Cano Francisco J. , Rodríguez-Lagunas Maria J. , Cambras Trinitat , Pastor-Soplin Santiago , Best Iván , Castell Margarida , Massot-Cladera Malén
Published: May 2020

Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals’ motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
Identification of Alternative Mitochondrial Electron Transport Pathway Components in Chickpea Indicates a Differential Response to Salinity Stress between Cultivars
Authors: Sweetman Crystal , Miller Troy K. , Booth Nicholas J. , Shavrukov Yuri , Jenkins Colin L.D. , Soole Kathleen L. , Day David A.
Published: May 2020

All plants contain an alternative electron transport pathway (AP) in their mitochondria, consisting of the alternative oxidase (AOX) and type 2 NAD(P)H dehydrogenase (ND) families, that are thought to play a role in controlling oxidative stress responses at the cellular level. These alternative electron transport components have been extensively studied in plants like Arabidopsis and stress inducible isoforms identified, but we know very little about them in the important crop plant chickpea. Here we identify AP components in chickpea (Cicer arietinum) and explore their response to stress at the transcript level. Based on sequence similarity with the functionally characterized proteins of Arabidopsis thaliana, five putative internal (matrix)-facing NAD(P)H dehydrogenases (CaNDA1-4 and CaNDC1) and four putative external (inter-membrane space)-facing NAD(P)H dehydrogenases (CaNDB1-4) were identified in chickpea. The corresponding activities were demonstrated for the first time in purified mitochondria of chickpea leaves and roots. Oxidation of matrix NADH generated from malate or glycine in the presence of the Complex I inhibitor rotenone was high compared to other plant species, as was oxidation of exogenous NAD(P)H. In leaf mitochondria, external NADH oxidation was stimulated by exogenous calcium and external NADPH oxidation was essentially calcium dependent. However, in roots these activities were low and largely calcium independent. A salinity experiment with six chickpea cultivars was used to identify salt-responsive alternative oxidase and NAD(P)H dehydrogenase gene transcripts in leaves from a three-point time series. An analysis of the Na:K ratio and Na content separated these cultivars into high and low Na accumulators. In the high Na accumulators, there was a significant up-regulation of CaAOX1, CaNDB2, CaNDB4, CaNDA3 and CaNDC1 in leaf tissue under long term stress, suggesting the formation of a stress-modified form of the mitochondrial electron transport chain (mETC) in leaves of these cultivars. In particular, stress-induced expression of the CaNDB2 gene showed a striking positive correlation with that of CaAOX1 across all genotypes and time points. The coordinated salinity-induced up-regulation of CaAOX1 and CaNDB2 suggests that the mitochondrial alternative pathway of respiration is an important facet of the stress response in chickpea, in high Na accumulators in particular, despite high capacities for both of these activities in leaf mitochondria of non-stressed chickpeas.
MiRNAs Expression Profiling of Bovine (Bos taurus) Testes and Effect of bta-miR-146b on Proliferation and Apoptosis in Bovine Male Germline Stem Cells
Authors: Gao Yuan , Wu Fei , Ren Yaxuan , Zhou Zihui , Chen Ningbo , Huang Yongzhen , Lei Chuzhao , Chen Hong , Dang Ruihua
Published: May 2020

Spermatogenesis is a complex biological process regulated by well-coordinated gene regulation, including MicroRNAs (miRNAs). miRNAs are endogenous non-coding ribonucleic acids (ncRNAs) that mainly regulate the gene expression at post-transcriptional levels. Several studies have reported miRNAs expression in bull sperm and the process of spermatogenic arrest in cattle and yak. However, studies for the identification of differential miRNA expression and its mechanisms during the developmental stages of testis still remain uncertain. In the current study, we comprehensively analyzed the expression of miRNA in bovine testes at neonatal (3 days after birth, n = 3) and mature (13 months, n = 3) stages by RNA-seq. Moreover, the role of bta-miR-146b was also investigated in regulating the proliferation and apoptosis of bovine male germline stem cells (mGSCs) followed by a series of experiments. A total of 652 miRNAs (566 known and 86 novel miRNAs) were identified, whereas 223 miRNAs were differentially expressed between the two stages. Moreover, an elevated expression level of bta-miR-146b was found in bovine testis among nine tissues, and the functional studies indicated that the overexpression of bta-miR-146b inhibited the proliferation of bovine mGSCs and promoted apoptosis. Conversely, regulation of bta-miR-146b inhibitor promoted bovine mGSCs proliferation. This study provides a basis for understanding the regulation roles of miRNAs in bovine testis development and spermatogenesis.
Bearing Strength of Crumb Rubber Concrete under Partial Area Loading
Authors: Xu Xiaoqing , Zhang Zhigang , Hu Yangao , Wang Xin
Published: May 2020

The application of waste tire rubber as aggregates in concrete can help to reduce carbon emissions and achieve green gross domestic product (GDP). However, civil engineers still have concerns about using rubberized concrete in structural members. For the safety of structures, the bearing strength of concrete is a very important parameter to be considered in the design. This paper presented the first experimental and numerical study on the bearing strength of crumb rubber concrete. Prisms of both normal concrete and crumb rubber concrete were tested with loading plates of varying sizes. The test results show that the failure modes and deformation behavior of crumb rubber concrete specimens with different rubber contents were similar to those of normal concrete, and the bearing strength of crumb rubber concrete can be well predicted by current standards for normal concrete. Finite element analysis was performed to further determine the effect of rubber content on the bearing strength of concrete. Proper parameter values for modeling crumb rubber concrete by the concrete damaged plasticity model were investigated. Through the numerical analysis, the reason the rubber content does not have an important effect on the bearing strength of crumb rubber concrete with similar compressive strength was found to be that the influence of rubber content on the tri-axial compression behavior of concrete and the ratio of concrete tensile strength to compressive strength is small. The experimental and numerical results presented in this study provide the insights needed to guide the design of structures utilizing crumb rubber concrete.
Evaluation of Surface Topography after Face Turning of CoCr Alloys Fabricated by Casting and Selective Laser Melting
Authors: Krawczyk Marta Beata , Królikowski Marcin Andrzej , Grochała Daniel , Powałka Bartosz , Figiel Paweł , Wojciechowski Szymon
Published: May 2020

The machinability of hard-to-cut CoCr alloys manufactured by Selective Laser Melting (SLM) technology is not yet sufficiently studied. Therefore, this work focuses on evaluation of surface texture formation during face turning of CoCr alloy. As part of the research, two specimen types were subject to comparison: made with the application of conventional casting and manufactured by additive manufacturing—SLM. A number of analytical and experimental methods were employed to describe the specimen composition and morphology, as: X-Ray Diffraction Analysis (XRD), optical metallurgical microscopy, confocal optical microscopy, and Vickers hardness HV0.1 measurements. In the next stage, the measurements of surface topographies formed during turning in a range of variable cutting speeds and feeds were carried out. Ultimately the multi-factor MANOVA (Multivariate Analysis of Variance) illustrating the influence of manufacturing technology, cutting speed, and feed ratio on selected surface parameters of samples was made. It has been demonstrated that during face turning with feeds up to 0.15 mm/rev, the similar values of surface roughness height and material ratio curve parameters were reached for both tested CoCr alloys. However, in a range of higher feed values, the surface quality of CoCr samples fabricated by SLM was lower than that reached for CoCr after casting process.
Study on the Elastic–Plastic Correlation of Low-Cycle Fatigue for Variable Asymmetric Loadings
Authors: Zhang Junhong , Li Weidong , Dai Huwei , Liu Nuohao , Lin Jiewei
Published: May 2020

The mean stress effect in fatigue life varies by material and loading conditions. Therefore, a classical low cycle fatigue (LCF) model based on mean stress correction shows limits in asymmetric loading cases in both accuracy and applicability. In this paper, the effect of strain ratio (R) on LCF life is analyzed and a strain ratio-based model is presented for asymmetric loading cases. Two correction factors are introduced to express correlations between strain ratio and fatigue strength coefficient and between strain ratio and fatigue ductility coefficient. Verifications are conducted through four materials under different strain ratios: high-pressure tubing steel (HPTS), 2124-T851 aluminum alloy, epoxy resin and AZ61A magnesium alloy. Compared with current widely used LCF models, the proposed model shows a better life prediction accuracy and higher potential in implementation in symmetric and asymmetric loading cases for different materials. It is also found that the strain ratio-based correction is able to consider the damage of ratcheting strain that the mean stress-based models cannot.
Comparative Analysis of Fatigue Energy Characteristics of S355J2 Steel Subjected to Multi-Axis Loads
Authors: Lachowicz Cyprian T. , Owsiński Robert
Published: May 2020

In this study, a novel test system for estimating bending and torsion fatigue under selectable kinematic and dynamic loading modes was constructed. Using S355J2 steel specimens, a series of tests were conducted to determine material sensitivity to different load paths and loading modes. The experimental results were supplemented with the results of numerical analyses, on the basis of which the components of strain and stress tensors for subsequent analyses were determined in the entire working part of the specimen. An original method for determining specific strain energy components was used. The experimental results showed the grouping of data according to the mode of loading chosen. This could signify that the selected fatigue models are sensitive to certain loading scenarios. We performed in-depth data analysis and complex numerical simulations, formulating likely explanations for the observed effect.
Blown-Up Hirzebruch Surfaces and Special Divisor Classes
Authors: Lee Jae-Hyouk , Shin YongJoo
Published: May 2020

We work on special divisor classes on blow-ups F p , r of Hirzebruch surfaces over the field of complex numbers, and extend fundamental properties of special divisor classes on del Pezzo surfaces parallel to analogous ones on surfaces F p , r . We also consider special divisor classes on surfaces F p , r with respect to monoidal transformations and explain the tie-ups among them contrast to the special divisor classes on del Pezzo surfaces. In particular, the fundamental properties of quartic rational divisor classes on surfaces F p , r are studied, and we obtain interwinded relationships among rulings, exceptional systems and quartic rational divisor classes along with monoidal transformations. We also obtain the effectiveness for the rational divisor classes on F p , r with positivity condition.
Discrimination of Isointense Bitter Stimuli in a Beer Model System
Authors: Higgins Molly J. , Hayes John E.
Published: May 2020

Prior work suggests humans can differentiate between bitter stimuli in water. Here, we describe three experiments that test whether beer consumers can discriminate between different bitterants in beer. In Experiment 1 (n = 51), stimuli were intensity matched; Experiments 2 and 3 were a difference from control (DFC)/check-all-that-apply (CATA) test (n = 62), and an affective test (n = 81). All used a commercial non-alcoholic beer spiked with Isolone (a hop extract), quinine sulfate dihydrate, and sucrose octaacetate (SOA). In Experiment 1, participants rated intensities on general labeled magnitude scales (gLMS), which were analyzed via ANOVA. In Experiment 2, participants rated how different samples were from a reference of Isolone on a 7-point DFC scale, and endorsed 13 attributes in a CATA task. DFC data were analyzed via ANOVA with Dunnett’s test to compare differences relative to a blind reference, and CATA data were analyzed via Cochran’s Q test. In Experiment 3, liking was assessed on labeled affective magnitude scales, and samples were also ranked. Liking was analyzed via ANOVA and rankings were analyzed with a Cochran–Mantel–Haenszel test. Experiment 1 confirmed that samples were isointense. In Experiment 2, despite being isointense, both quinine (p = 0.04) and SOA (p = 0.03) were different from Isolone, but no significant effects were found for CATA descriptors (all p values > 0.16). In Experiment 3, neither liking (p = 0.16) or ranking (p = 0.49) differed. Collectively, these data confirm that individuals can discriminate perceptually distinct bitter stimuli in beer, as shown previously in water, but these differences cannot be described semantically, and they do not seem to influence hedonic assessments.
Strengthening the Immune System and Reducing Inflammation and Oxidative Stress through Diet and Nutrition: Considerations during the COVID-19 Crisis
Authors: Iddir Mohammed , Brito Alex , Dingeo Giulia , Fernandez Del Campo Sofia Sosa , Samouda Hanen , La Frano Michael R. , Bohn Torsten
Published: May 2020

The coronavirus-disease 2019 (COVID-19) was announced as a global pandemic by the World Health Organization. Challenges arise concerning how to optimally support the immune system in the general population, especially under self-confinement. An optimal immune response depends on an adequate diet and nutrition in order to keep infection at bay. For example, sufficient protein intake is crucial for optimal antibody production. Low micronutrient status, such as of vitamin A or zinc, has been associated with increased infection risk. Frequently, poor nutrient status is associated with inflammation and oxidative stress, which in turn can impact the immune system. Dietary constituents with especially high anti-inflammatory and antioxidant capacity include vitamin C, vitamin E, and phytochemicals such as carotenoids and polyphenols. Several of these can interact with transcription factors such as NF-kB and Nrf-2, related to anti-inflammatory and antioxidant effects, respectively. Vitamin D in particular may perturb viral cellular infection via interacting with cell entry receptors (angiotensin converting enzyme 2), ACE2. Dietary fiber, fermented by the gut microbiota into short-chain fatty acids, has also been shown to produce anti-inflammatory effects. In this review, we highlight the importance of an optimal status of relevant nutrients to effectively reduce inflammation and oxidative stress, thereby strengthening the immune system during the COVID-19 crisis.
Inflammation Adjustment by Two Methods Decreases the Estimated Prevalence of Zinc Deficiency in Malawi
Authors: Likoswe Blessings H. , Phiri Felix P. , Broadley Martin R. , Joy Edward J. M. , Patson Noel , Maleta Kenneth M. , Phuka John C.
Published: May 2020

Serum zinc concentration (SZC) is used widely to assess population-level zinc status. Its concentration decreases during inflammatory responses, which can affect the interpretation of the results. This study aimed to re-estimate the prevalence of zinc deficiency in Malawi based on the 2015–2016 Malawi Micronutrient Survey (MNS) data, by adjusting SZC measures with markers of inflammation. SZC and inflammation data from 2760 participants were analysed. Adjustments were made using: (1) The Internal Correction Factor (ICF) method which used geometric means, and (2) The Biomarkers Reflecting Inflammation and Nutritional Determinants of Anemia (BRINDA) method, which used linear regression. Mean SZC values increased significantly when adjustments were made by either ICF or BRINDA (p < 0.001). The national prevalence of zinc deficiency decreased from 62% to 59%, after ICF adjustment, and to 52% after BRINDA adjustment. ICF and BRINDA values of SZC were highly correlated (p < 0.001, r = 0.99), but a Bland–Altman plot showed a lack of agreement between the two methods (bias of 2.07 µg/dL). There was no association between the adjusted SZC and stunting, which is a proxy indicator for zinc deficiency. Inflammation adjustment of SZC, using ICF or BRINDA, produces lower estimates of zinc deficiency prevalence, but the lack of agreement between the adjustment methods warrants further research. Furthermore, the lack of association between SZC and stunting highlights the need to explore other biomarkers and proxies of population zinc assessment. This study demonstrates the importance of considering inflammatory confounders when reporting SZC, to ensure accuracy and to support policy decision making.
Cardiometabolic Benefits of a Weight-Loss Mediterranean Diet/Lifestyle Intervention in Patients with Obstructive Sleep Apnea: The “MIMOSA” Randomized Clinical Trial
Authors: Georgoulis Michael , Yiannakouris Nikos , Kechribari Ioanna , Lamprou Kallirroi , Perraki Eleni , Vagiakis Emmanouil , Kontogianni Meropi D
Published: May 2020

Although continuous positive airway pressure (CPAP) is the first-line treatment for obstructive sleep apnea (OSA), its cardiometabolic benefits are questionable. Our aim was to explore whether the combination of a weight-loss Mediterranean diet/lifestyle intervention with OSA standard care leads to greater cardiometabolic improvements compared with standard care alone. We randomly assigned 187 adult, overweight, polysomnography-diagnosed moderate-to-severe OSA patients to a standard care group (SCG, n = 65), a Mediterranean diet group (MDG, n = 62) or a Mediterranean lifestyle group (MLG, n = 60). All three groups were prescribed with CPAP. Additionally, the SCG only received brief written healthy lifestyle advice, while intervention arms were subjected to a six-month weight-loss behavioral intervention based on the Mediterranean diet. The MLG also received guidance for improving physical activity and sleep habits. Glucose metabolism indices, blood lipids, liver enzymes and blood pressure improved only in intervention arms, and were significantly lower compared to the SCG post-intervention (all p < 0.05). The age-, sex-, baseline- and CPAP use-adjusted relative risk (95% confidence interval) of metabolic syndrome was 0.58 (0.34–0.99) for the MDG and 0.30 (0.17–0.52) for the MLG compared to the SCG. The MLG additionally presented a lower relative risk of metabolic syndrome compared to the MDG (0.52 (0.30–0.89)). After further adjustment for body-weight change, a lower relative risk of metabolic syndrome was still evident for the MLG compared to the SCG. In conclusion, although standard care alone does not improve OSA patients’ cardiometabolic profile, its combination with a weight-loss Mediterranean diet/lifestyle intervention leads to significant cardiometabolic benefits.
The Effects of Aronia melanocarpa Extract on Testosterone-Induced Benign Prostatic Hyperplasia in Rats, and Quantitative Analysis of Major Constituents Depending on Extract Conditions
Authors: Kim Na-Hyun , Jegal Jonghwan , Kim Yun Na , Heo Jeong-Doo , Rho Jung-Rae , Yang Min Hye , Jeong Eun Ju
Published: May 2020

This study aimed to investigate the beneficial effects of A. melanocarpa on testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) in Wistar rats. Moreover, the bioactive constituents in the extract were determined using LC/MS and HPLC analyses. The dried fruits of A. melanocarpa were extracted using accelerated solvent extraction (ASE) under different extract conditions (temperature, 30 °C or 100 °C; extract solvent, 60% or 100% ethanol) to yield four extracts (T1~T4). Of the four A. melanocarpa extracts, T1 extracted under the condition of 100% ethanol/low temperature (30 °C) exhibited the greatest inhibitory activity on TP-induced prostatic hyperplasia in rats. The administration of T1 (100 mg/kg body weight, p.o.) for six weeks attenuated TP-induced prostate enlargement and reduced the levels of dihydrotestosterone (DHT) and 5α-reductase in both serum and prostate tissue. The suppression of PCNA mRNA expression in prostate tissue was remarkable in T1-treated rats. In LC/MS analysis, the levels of main anthocyanins and phenolics were significantly higher in T1 than in the other extracts. Furthermore, the quantitative study showed that the contents of cyanidin-3-glucose and cyanidin-3-xylose in T1 exhibited 1.27~1.67 and 1.10~1.26 folds higher compared to those in the other extracts. These findings demonstrated that A. melanocarpa extract containing anthocyanins as bioactive constituents attenuated the development of testosterone-induced prostatic hyperplasia, and suggested that this extract has therapeutic potential to treat prostate enlargement and BPH.
Dietary Fibres Differentially Impact on the Production of Phenolic Acids from Rutin in an In Vitro Fermentation Model of the Human Gut Microbiota
Authors: Havlik Jaroslav , Marinello Vittoria , Gardyne Andrew , Hou Min , Mullen William , Morrison Douglas J. , Preston Thomas , Combet Emilie , Edwards Christine A.
Published: May 2020

Polyphenols are often ingested alongside dietary fibres. They are both catabolised by, and may influence, the intestinal microbiota; yet, interactions between them and the impact on their resultant microbial products are poorly understood. Dietary fibres (inulin, pectin, psyllium, pyrodextrin, wheat bran, cellulose—three doses) were fermented in vitro with human faeces (n = 10) with and without rutin (20 µg/mL), a common dietary flavonol glycoside. Twenty-eight phenolic metabolites and short chain fatty acids (SCFA) were measured over 24 h. Several phenolic metabolites were produced during fibre fermentation, without rutin. With rutin, 3,4-dihydroxyphenylacetic acid (3,4diOHPAA), 3-hydroxyphenylacetic acid (3OHPAA), 3-(3 hydroxyphenyl)propionic acid (3OHPPA) and 3-(3,4-dihydroxyphenyl)propionic acid (3,4diOHPPA; DOPAC) were produced, with 3,4diOHPAA the most abundant, confirmed by fermentation of 13C labelled quercetin. The addition of inulin, wheat bran or pyrodextrin increased 3,4diOHPAA 2 2.5-fold over 24 h (p < 0.05). Rutin affected SCFA production, but this depended on fibre, fibre concentration and timepoint. With inulin, rutin increased pH at 6 h from 4.9 to 5.6 (p = 0.01) but increased propionic, butyric and isovaleric acid (1.9, 1.6 and 5-fold, p < 0.05 at 24 h). Interactions between fibre and phenolics modify production of phenolic acids and SCFA and may be key in enhancing health benefits.
Association between Nutrient-Based Dietary Patterns and Bladder Cancer in Italy
Authors: Edefonti Valeria , La Vecchia Carlo , Di Maso Matteo , Crispo Anna , Polesel Jerry , Libra Massimo , Parpinel Maria , Serraino Diego , Ferraroni Monica , Bravi Francesca
Published: May 2020

Limited knowledge is available on dietary patterns and bladder cancer risk. We analyzed data from an Italian case-control study carried out between 2003 and 2014, including 690 incident bladder cancer cases and 665 hospital-controls. We derived nutrient-based dietary patterns applying principal component factor analysis on 28 selected nutrients. We categorized factor scores according to quartiles, and estimated the odds ratios (ORs) and the corresponding 95% confidence intervals (CIs) through logistic regression models, adjusted for major confounding factors. We identified four dietary patterns named “Animal products”, “Vitamins and fiber”, “Starch-rich”, and “Animal unsaturated fatty acids”. We found an inverse association between the “Vitamins and fiber” pattern and bladder cancer (OR = 0.70, 95% CI: 0.48–0.99, IV versus I quartile category). Inverse relationships of borderline significance were also found for the “Animal products” and the “Animal unsaturated fatty acids” dietary patterns. No significant association was evident for the “Starch-rich” pattern. The current study allowed us to identify major dietary patterns in this Italian population. Our study confirms available evidence and shows that scoring high on a fruit-and-vegetables pattern provides beneficial effects on bladder cancer risk.
Effect of a Multispecies Probiotic on Intestinal and Skin Colonization by Multidrug-Resistant Gram-Negative Bacteria in Patients in a Long-Term Care Facility: A Pilot Study
Authors: Zollner-Schwetz Ines , Scarpatetti Monika , Pichler Gerald , Pux Christian , Klymiuk Ingeborg , Trajanoski Slave , Krause Robert
Published: May 2020

Residents in long-term care facilities (LTCFs) are frequently colonized by multidrug-resistant Gram-negative bacteria, putting them at risk for subsequent infections. We aimed to evaluate the effect of the multispecies probiotic Omnibiotic10AAD® on the intestinal and inguinal skin colonization of patients by multidrug-resistant Gram-negative bacteria in LTCFs. Patients colonized by multidrug-resistant Gram-negative bacteria received a 12 week oral course of Omnibiotic10AAD®. Inguinal swabs and stool samples were collected during and after treatment for microbiological and microbiome analysis. The median age of patients was 76 years. Twelve patients completed the pilot study. Intestinal colonization was reduced to 42% of patients 8 weeks after the end of treatment, but increased to 66% 24 weeks after the end of probiotic treatment. Colonization of inguinal skin was lowest during probiotic treatment and increased thereafter. Fecal microbiome analysis revealed statistically significant increases of the genus Enterococcus comparing start and end of probiotic treatment. In conclusion, a 12 week course of a multispecies probiotic led to a transient reduction of intestinal colonization 8 weeks after the end of treatment. The findings of our pilot study warrant further research in the area of probiotics and intestinal colonization by multidrug-resistant bacteria.
Citrus aurantium L. Dry Extracts Ameliorate Adipocyte Differentiation of 3T3-L1 Cells Exposed to TNFα by Down-Regulating miR-155 Expression
Authors: Campitelli Michele , Desiderio Antonella , Cacace Giuseppe , Nigro Cecilia , Prevenzano Immacolata , Leone Alessia , de Simone Sonia , Campiglia Pietro , Formisano Pietro , Raciti Gregory A. , Beguinot Francesco , Miele Claudia
Published: May 2020

Citrus aurantium L. dry extracts (CAde) improve adipogenesis in vitro. These effects are dependent from an early modulation of CCAAT/enhancer-binding protein beta (C/Ebpβ) expression and cyclic Adenosine Monophosphate (cAMP) response element-binding protein (CREB) activation. C/Ebpβ and Creb are also targets of miR-155. This study investigated whether CAde regulates miR-155 expression in the early stages of adipogenesis and whether it ameliorates adipocyte differentiation of cells exposed to tumor necrosis factor-alpha (TNFα). Adipogenic stimuli (AS) were performed in 3T3-L1 pre-adipocytes treated with CAde, TNFα, or both. Gene and miRNA expression were determined by quantitative real-time PCR. Adipogenesis was evaluated by Oil-Red O staining. CAde treatment enhanced AS effects during the early adipogenesis phases by further down-regulating miR-155 expression and increasing both C/Ebpβ and Creb mRNA and protein levels. At variance, TNFα inhibited 3T3-L1 adipogenesis and abolished AS effects on miR-155, C/Ebpβ, and Creb expression. However, in cells exposed to TNFα, CAde improved adipocyte differentiation and restored the AS effects on miRNA and gene expression at early time points. In conclusion, this study identified miR-155 down-regulation as part of the mechanism through which CAde enhances adipogenesis of pre-adipocytes in vitro. Furthermore, it provides evidence of CAde efficacy against TNFα negative effects on adipogenesis.
New Solutions of Laser-Induced Fluorescence for Oil Pollution Monitoring at Sea
Authors: Bukin Oleg , Proschenko Dmitry , Alexey Chekhlenok , Korovetskiy Denis , Bukin Ilya , Yurchik Viktoria , Sokolova Irina , Nadezhkin Andrey
Published: May 2020

Laser-induced fluorescence (LIF) spectral features for oil products of different states (solutions in the seawater and thin slicks) are discussed in this article. This research was done to evaluate LIF application for the identification of oil products and the measurement of the volume of ocean pollution by bilge water disposal. It was found out that the form of LIF spectral distribution was changed depending on the oil product state (pure fuel, slick or solution). The LIF method was calibrated for the most common types of heavy and light marine fuels at the standard measurement method of solution concentrations and limit of detection (LoD) values were established for each type. The time dynamics of the solution spectra were researched, and the time change features were determined. The smallsized LIF sensor for the unmanned aerial vehicle (UAV) is described and aims to investigate the LIF for oil pollution at sea.
Uncertainty Analysis for RadCalNet Instrumented Test Sites Using the Baotou Sites BTCN and BSCN as Examples
Authors: Ma Lingling , Zhao Yongguang , Woolliams Emma R. , Dai Caihong , Wang Ning , Liu Yaokai , Li Ling , Wang Xinhong , Gao Caixia , Li Chuanrong , Tang Lingli
Published: May 2020

Vicarious calibration and validation techniques are important tools to ensure the long-term stability and inter-sensor consistency of satellite sensors making observations in the solar-reflective spectral domain. Automated test sites, which have continuous in situ monitoring of both ground reflectance and atmospheric conditions, can greatly increase the match-up possibilities for a wide range of space agency and commercial sensors. The Baotou calibration and validation test site in China provides operational high-accuracy and high-stability vicarious calibration and validation for high spatial resolution solar-reflective remote-sensing sensors. Two sites, given the abbreviations BTCN (an artificial site) and BSCN (a natural sandy site), have been selected as reference sites for the Committee on Earth Observation Satellites radiometric calibration network (RadCalNet). RadCalNet requires sites to provide data in a consistent format but does not specify the required operational conditions for a RadCalNet site. The two Baotou sites are the only sites to date that make spectral measurements for their continuous operation. One of the core principles of RadCalNet is that each site should have a metrologically rigorous uncertainty budget which also describes the site’s traceability to the international system of units, the SI. This paper shows a formalized metrological approach to determining and documenting the uncertainty budget and traceability of a RadCalNet site. This approach follows the Guide to the Expression of Uncertainty in Measurement. The paper describes the uncertainty analysis for bottom-of-atmosphere and top-of-atmosphere reflectance in the spectral region from 400 to 1000 nm for the Baotou sites and gives preliminary results for the uncertainty propagating this to top-of-atmosphere reflectance.
Azimuth Multichannel Reconstruction for Moving Targets in Geosynchronous Spaceborne–Airborne Bistatic SAR
Authors: Xu Wei , Wei Zhengbin , Huang Pingping , Tan Weixian , Liu Bo , Gao Zhiqi , Dong Yifan
Published: May 2020

In a multichannel geosynchronous spaceborne–airborne bistatic synthetic aperture radar (GEO-SA-BiSAR) system, the airborne receiver can obtain high-resolution microwave images with good signal-to-noise ratios (SNRs) by passively receiving echoes from the desired area. Since the Doppler modulation and range history of a moving target are obviously different from a stationary target, a signal geometry model for moving targets in multichannel GEO-SA-BiSAR is established in this paper. According to simulation results, the along track velocity introduces target defocusing in azimuth, and the slant range velocity mainly causes multiple false targets. To resolve these problems, a modified multichannel reconstruction method in azimuth channel GEO-SA-BiSAR is proposed according to the azimuth multichannel impulse response of the imaged moving target. Before azimuth multichannel raw data combination, both spatial-variant range cell migration correction (RCMC) and azimuth nonlinear chirp scaling (ANLCS) should be performed to reduce the influence of the range offset and lower the Doppler bandwidth of the whole raw data, respectively. Afterward, a novel azimuth multichannel reconstruction algorithm is carried out via the modified reconstruction matrix based on the estimated target velocity. The target slant range velocity estimation is implemented by introducing the signal intensity ratio (SIR). Compared with the conventional method for the stationary target to handle the raw data of the moving target, the false targets could be obviously suppressed by using the proposed approach. Imaging results on both simulated point and distributed scene targets validate the proposed multichannel reconstruction approach.
RL-AKF: An Adaptive Kalman Filter Navigation Algorithm Based on Reinforcement Learning for Ground Vehicles
Authors: Gao Xile , Luo Haiyong , Ning Bokun , Zhao Fang , Bao Linfeng , Gong Yilin , Xiao Yimin , Jiang Jinguang
Published: May 2020

Kalman filter is a commonly used method in the Global Navigation Satellite System (GNSS)/Inertial Navigation System (INS) integrated navigation system, in which the process noise covariance matrix has a significant influence on the positioning accuracy and sometimes even causes the filter to diverge when using the process noise covariance matrix with large errors. Though many studies have been done on process noise covariance estimation, the ability of the existing methods to adapt to dynamic and complex environments is still weak. To obtain accurate and robust localization results under various complex and dynamic environments, we propose an adaptive Kalman filter navigation algorithm (which is simply called RL-AKF), which can adaptively estimate the process noise covariance matrix using a reinforcement learning approach. By taking the integrated navigation system as the environment, and the opposite of the current positioning error as the reward, the adaptive Kalman filter navigation algorithm uses the deep deterministic policy gradient to obtain the most optimal process noise covariance matrix estimation from the continuous action space. Extensive experimental results show that our proposed algorithm can accurately estimate the process noise covariance matrix, which is robust under different data collection times, different GNSS outage time periods, and using different integration navigation fusion schemes. The RL-AKF achieves an average positioning error of 0.6517 m within 10 s GNSS outage for GNSS/INS integrated navigation system and 14.9426 m and 15.3380 m within 300 s GNSS outage for the GNSS/INS/Odometer (ODO) and the GNSS/INS/Non-Holonomic Constraint (NHC) integrated navigation systems, respectively.