bluuebunny/crossref_metadata_2025 huggingface dataset:
Technological Feature Assessment of Lactic Acid Bacteria Isolated from Cricket Powder’s Spontaneous Fermentation as Potential Starters for Cricket-Wheat Bread Production
Authors: Galli Viola , Venturi Manuel , Pini Niccolò , Granchi Lisa
Published: September 2020

The bacterial community profile of cricket powder highlighted the presence of four main genera: Bacteroides spp., Parabacteroides spp., Lactococcus spp., and Enterococcus spp. The spontaneous fermentation of cricket powder allowed for the isolation and characterization of seven lactic acid bacteria strains belonging to six species: Latilactobacillus curvatus, Lactiplantibacillus plantarum, Latilactobacillus sakei, Lactococcus garvieae, Weissella confusa, and Enterococcus durans. The strains were characterized and selected according to different technological properties. L. plantarum CR L1 and L. curvatus CR L13 showed the best performance in terms of general aminopeptidase activity, acidification, and growth rate in MRS broth and in dough with cricket powder and wheat flour, as well as robustness during consecutive backslopping. Thus, they were used as starter-mixed to produce sourdough to manufacture bread fortified with 20% cricket powder. The addition of cricket powder led to a significant increase of protein (up to 94%) and lipid content, from 0.7 up to 6 g/100 g of bread. Spontaneous fermentation represents a source of microbial diversity that can be exploited in order to obtain potential starters for food with innovative ingredients. Edible insects powder can be successfully added in leavened baked goods to enhance their nutritional value.
The Influence of Hippotherapy on the Body Posture in a Sitting Position among Children with Cerebral Palsy
Authors: Matusiak-Wieczorek Ewelina , Dziankowska-Zaborszczyk Elzbieta , Synder Marek , Borowski Andrzej
Published: September 2020

The purpose of this study was to assess the influence of hippotherapy (therapy with horses) on posture and body function among children with cerebral palsy. A case–control study included forty-five children aged 6–12 years, classified as Gross Motor Function Classification System (GMFCS) level I or II, with spastic diplegia or hemiplegia. The participants were randomly divided into three groups: study I (n = 15), study II (n = 15) and control (n = 15). The children from the study groups attended 30min hippotherapy sessions for 12 consecutive weeks, twice (study group I) or once (study group II) a week. The Sitting Assessment Scale (SAS) was used. A comparison of SAS showed an improvement in almost all the assessed categories among the children who participated in hippotherapy. In study group I, statistically significant differences were noted in the assessment of head position control, arm function (in both cases, p = 0.012) and trunk control (p = 0.005) and in study group II in the assessment of trunk control (p = 0.028). Hippotherapy has a positive influence on the body posture and function of individual body parts in a sitting position among children with cerebral palsy.
Physical Activity, Sport and Physical Education in Northern Ireland School Children: A Cross-Sectional Study
Authors: Connolly Sinead , Carlin Angela , Johnston Anne , Woods Catherine , Powell Cormac , Belton Sarahjane , O’Brien Wesley , Saunders Jean , Duff Christina , Farmer Orlagh , Murphy Marie
Published: September 2020

Internationally, insufficient physical activity (PA) is a major health concern. Children in Northern Ireland (NI) are recorded as having the lowest levels of PA in the United Kingdom (UK). To date, validated and representative data on the PA levels of NI school children are limited. The aim of this study was to provide surveillance data on self-reported PA, sport and physical education (PE) participation of school children in NI. Differences between genders and factors associated with PA were also examined. A representative sample of primary (n = 446) and post-primary (n = 1508) children was surveyed in school using validated self-report measures. Findings suggest that PA levels are low, with a minority of children (13%) meeting the PA guidelines (primary pupils 20%, post-primary pupils 11%). NI school children have lower levels of PA, PE and sports participation than UK and European peers. A trend of age-related decline across all the domains of PA was apparent. The data presented highlighted that females are less likely to achieve PA guidelines, children from lower socio-economic background participate in school and community sport less often, and that enjoyment and social support are important variables in PA adherence. Policy solutions that would support implementation e.g., mandatory minimum PE time, whole school approaches to PA promotion and targeted investment in schools, particularly in areas of deprivation and for females, are suggested.
Infectious or Recovered? Optimizing the Infectious Disease Detection Process for Epidemic Control and Prevention Based on Social Media
Authors: Shan Siqing , Yan Qi , Wei Yigang
Published: September 2020

Detecting the period of a disease is of great importance to building information management capacity in disease control and prevention. This paper aims to optimize the disease surveillance process by further identifying the infectious or recovered period of flu cases through social media. Specifically, this paper explores the potential of using public sentiment to detect flu periods at word level. At text level, we constructed a deep learning method to classify the flu period and improve the classification result with sentiment polarity. Three important findings are revealed. Firstly, bloggers in different periods express significantly different sentiments. Blogger sentiments in the recovered period are more positive than in the infectious period when measured by the interclass distance. Secondly, the optimized disease detection process can substantially improve the classification accuracy of flu periods from 0.876 to 0.926. Thirdly, our experimental results confirm that sentiment classification plays a crucial role in accuracy improvement. Precise identification of disease periods enhances the channels for the disease surveillance processes. Therefore, a disease outbreak can be predicted credibly when a larger population is monitored. The research method proposed in our work also provides decision making reference for proactive and effective epidemic control and prevention in real time.
Antithrombotic Preventive Medication Prescription Redemption and Socioeconomic Status in Hungary in 2016: A Cross-Sectional Study
Authors: Juhász Attila , Nagy Csilla , Varga Orsolya , Boruzs Klára , Csernoch Mária , Szabó Zoltán , Ádány Róza
Published: September 2020

This work was designed to investigate antithrombotic drug utilization and its link with the socioeconomic characteristics of specific population groups in Hungary by a comparative analysis of data for prescriptions by general practitioners and the redeemed prescriptions for antithrombotic drugs. Risk analysis capabilities were applied to estimate the relationships between socioeconomic status, which was characterized by quintiles of a multidimensional composite indicator (deprivation index), and mortality due to thromboembolic diseases as well as antithrombotic medications for the year 2016 at the district level in Hungary. According to our findings, although deprivation is a significant determinant of mortality due to thromboembolic diseases, clusters can be identified that represent exemptions to this rule: an eastern part of Hungary, consisting of two highly deprived counties, had significantly lower mortality than the country average; by contrast, the least-deprived northwestern part of the country, consisting of five counties, had significantly higher mortality than the country average. The fact that low socioeconomic status in general and poor adherence to antithrombotic drugs irrespective of socioeconomic status were associated with increased mortality indicates the importance of more efficient control of preventive medication and access to healthcare in all districts of the country to reduce mortality due to thromboembolic diseases.
Social and Demographic Patterns of Health-Related Internet Use Among Adults in the United States: A Secondary Data Analysis of the Health Information National Trends Survey
Authors: Calixte Rose , Rivera Argelis , Oridota Olutobi , Beauchamp William , Camacho-Rivera Marlene
Published: September 2020

National surveys of U.S. adults have observed significant increases in health-related internet use (HRIU), but there are documented disparities. The study aims to identify social and demographic patterns of health-related internet use among U.S. adults. Using data from the Health Information National Trends Survey (HINTS) 4 cycle 3 and HINTS 5 cycle 1, we examined HRIU across healthcare, health information seeking, and participation on social media. Primary predictors were gender, race/ethnicity, age, education, income, and nativity with adjustments for smoking and survey year. We used multivariable logistic regression with survey weights to identify independent predictors of HRIU. Of the 4817 respondents, 43% had used the internet to find a doctor; 80% had looked online for health information. Only 20% had used social media for a health issue; 7% participated in an online health support group. In multivariable models, older and low SES participants were significantly less likely to use the internet to look for a provider, use the internet to look for health information for themselves or someone else, and less likely to use social media for health issues. Use of the internet for health-related purposes is vast but varies significantly by demographics and intended use.
Farm to Early Care and Education Programming: A Descriptive Study of Challenges and Opportunities to Promote Healthful Foods to Young Children
Authors: McCloskey Morgan L. , Kesterson Hannah , Mena Noereem Z. , Dellaport Jennifer , Bellows Laura L.
Published: September 2020

Interest in farm to early care and education (ECE) programming, which consists of gardening, nutrition education, and local food procurement, has been growing in the United States, as it may be a promising technique for promoting healthful foods to young children. However, there is limited information about current farm to ECE efforts in specific states, including Colorado, to support funding and resource needs. An online survey was distributed to licensed Colorado ECE providers in two phases to understand current participation in the farm to ECE as well as provider perspectives on benefits and barriers to programming. A total of 250 surveys were completed. Approximately 60% of ECE facilities participated in gardening and nutrition education with providers almost unanimously agreeing on the child-centric benefits of programming. Fewer facilities (37%) participated in local food procurement likely due to significant time, cost, and knowledge barriers. To increase participation in farm to ECE as a technique for promoting healthful foods to young children, future efforts should focus on innovative solutions to reduce ECE-specific barriers.
Association between Perceived Neighborhood Built Environment and Walking and Cycling for Transport among Inhabitants from Latin America: The ELANS Study
Authors: Ferrari Gerson , Oliveira Werneck André , Rodrigues da Silva Danilo , Kovalskys Irina , Gómez Georgina , Rigotti Attilio , Yadira Cortés Sanabria Lilia , García Martha , Pareja Rossina , Herrera-Cuenca Marianella , Zimberg Ioná , Guajardo Viviana , Pratt Michael , Cofre Bolados Cristian , Saldía Emilio , Pires Carlos , Marques Adilson , Peralta Miguel , Rossato de Victo Eduardo , Fisberg Mauro ,   on behalf of the ELANS Study Group
Published: September 2020

Purpose: This study aimed to examine the associations of the perceived neighborhood built environment with walking and cycling for transport in inhabitants from Latin American countries. Methods: This cross-sectional study involved 9218 participants (15–65 years) from the Latin American Study of Nutrition and Health, which included a nationally representative sample of eight countries. All participants completed the International Physical Activity Questionnaire-Long Form for measure walking and cycling for transport and the Neighborhood Environment Walkability Scale-Abbreviated. Furthermore, perceived proximity from home to public open spaces and shopping centers was assessed. Results: Perceived land use mix-access (OR: 1.32; 95%CI: 1.16,1.50) and the existence of many alternative routes in the neighbourhood (1.09 1.01,1.17) were associated with higher odds of reporting any walking for transport (≥10 min/week). Perceived slow speed of traffic (1.88 1.82,1.93) and few drivers exceeding the speed limits (1.92; 1.86,1.98) were also related to higher odds of reporting any walking for transport. The odds of reporting any cycling for transport (≥10 min/week) were higher in participants perceiving more walking/cycling facilities (1.87 1.76,1.99), and better aesthetics (1.22 1.09,1.38). Conclusions: Dissimilar perceived neighborhood built environment characteristics were associated with walking and cycling for transport among inhabitants from Latin America.
Transition Trajectories: Contexts, Difficulties and Consequences Reported by Young Transgender and Non-Binary Spaniards
Authors: Magalhães Mariana , Aparicio-García Marta E. , García-Nieto Isidro
Published: September 2020

The transition process can have a significant impact on young transgender and non-binary individuals (TNBI), especially regarding their mental health. As such, this study aimed to explore the encountered difficulties and expectations of 225 young adults, between 14 and 25 years old, who identify themselves as TNBI. Four different aspects were analyzed: difficulties related to the transition process; main current difficulties; if the current difficulties are due to the participants’ sexual orientation or gender identity; and if participants feel the need of changing something in their lives. Several obstacles either before, during or after the transition process were identified; and these obstacles have serious consequences in TBNI’s mental health. Questions regarding legislation, discrimination, transphobia, lack of social support, the pathologization of transgenderism, low self-esteem, anxiety symptomatology, among others, were pointed out by the participants. The narratives collected and analyzed in the present study appear as important inputs to the literature, which can be translated into the basis of further investigations and the improvement of professional practices in the health field.
Functional and Pharmacological Comparison of Human, Mouse, and Rat Organic Cation Transporter 1 toward Drug and Pesticide Interaction
Authors: Floerl Saskia , Kuehne Annett , Hagos Yohannes
Published: September 2020

Extrapolation from animal to human data is not always possible, because several essential factors, such as expression level, localization, as well as the substrate selectivity and affinity of relevant transport proteins, can differ between species. In this study, we examined the interactions of drugs and pesticides with the clinically relevant organic cation transporter hOCT1 (SLC22A1) in comparison to the orthologous transporters from mouse and rat. We determined Km-values (73 ± 7, 36 ± 13, and 57 ± 5 µM) of human, mouse and rat OCT1 for the commonly used substrate 1-methyl-4-phenylpyridinium (MPP) and IC50-values of decynium22 (12.1 ± 0.8, 5.3 ± 0.4, and 10.5 ± 0.4 µM). For the first time, we demonstrated the interaction of the cationic fungicides imazalil, azoxystrobin, prochloraz, and propamocarb with human and rodent OCT1. Drugs such as ketoconazole, clonidine, and verapamil showed substantial inhibitory potential to human, mouse, and rat OCT1 activity. A correlation analysis of hOCT1 versus mouse and rat orthologs revealed a strong functional correlation between the three species. In conclusion, this approach shows that transporter interaction data are in many cases transferable between rodents and humans, but potential species differences for other drugs and pesticides could not be excluded, though it is recommendable to perform functional comparisons of human and rodent transporters for new molecular entities.
Systematic Analysis of Cold Stress Response and Diurnal Rhythm Using Transcriptome Data in Rice Reveals the Molecular Networks Related to Various Biological Processes
Authors: Hong Woo-Jong , Jiang Xu , Ahn Hye Ryun , Choi Juyoung , Kim Seong-Ryong , Jung Ki-Hong
Published: September 2020

Rice (Oryza sativa L.), a staple crop plant that is a major source of calories for approximately 50% of the human population, exhibits various physiological responses against temperature stress. These responses are known mechanisms of flexible adaptation through crosstalk with the intrinsic circadian clock. However, the molecular regulatory network underlining this crosstalk remains poorly understood. Therefore, we performed systematic transcriptome data analyses to identify the genes involved in both cold stress responses and diurnal rhythmic patterns. Here, we first identified cold-regulated genes and then identified diurnal rhythmic genes from those (119 cold-upregulated and 346 cold-downregulated genes). We defined cold-responsive diurnal rhythmic genes as CD genes. We further analyzed the functional features of these CD genes through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses and performed a literature search to identify functionally characterized CD genes. Subsequently, we found that light-harvesting complex proteins involved in photosynthesis strongly associate with the crosstalk. Furthermore, we constructed a protein–protein interaction network encompassing four hub genes and analyzed the roles of the Stay-Green (SGR) gene in regulating crosstalk with sgr mutants. We predict that these findings will provide new insights in understanding the environmental stress response of crop plants against climate change.
3D-Printed Ceramic Bone Scaffolds with Variable Pore Architectures
Authors: Lim Ho-Kyung , Hong Seok-Jin , Byeon Sun-Ju , Chung Sung-Min , On Sung-Woon , Yang Byoung-Eun , Lee Jong-Ho , Byun Soo-Hwan
Published: September 2020

This study evaluated the mechanical properties and bone regeneration ability of 3D-printed pure hydroxyapatite (HA)/tricalcium phosphate (TCP) pure ceramic scaffolds with variable pore architectures. A digital light processing (DLP) 3D printer was used to construct block-type scaffolds containing only HA and TCP after the polymer binder was completely removed by heat treatment. The compressive strength and porosity of the blocks with various structures were measured; scaffolds with different pore sizes were implanted in rabbit calvarial models. The animals were observed for eight weeks, and six animals were euthanized in the fourth and eighth weeks. Then, the specimens were evaluated using radiological and histological analyses. Larger scaffold pore sizes resulted in enhanced bone formation after four weeks (p < 0.05). However, in the eighth week, a correlation between pore size and bone formation was not observed (p > 0.05). The findings showed that various pore architectures of HA/TCP scaffolds can be achieved using DLP 3D printing, which can be a valuable tool for optimizing bone-scaffold properties for specific clinical treatments. As the pore size only influenced bone regeneration in the initial stage, further studies are required for pore-size optimization to balance the initial bone regeneration and mechanical strength of the scaffold.
Lens Connexin Channels Show Differential Permeability to Signaling Molecules
Authors: Brink Peter R. , Valiunas Virginijus , White Thomas W.
Published: September 2020

Gap junction channels mediate the direct intercellular passage of small ions as well as larger solutes such as second messengers. A family of proteins called connexins make up the subunits of gap junction channels in chordate animals. Each individual connexin forms channels that exhibit distinct permeability to molecules that influence cellular signaling, such as calcium ions, cyclic nucleotides, or inositol phosphates. In this review, we examine the permeability of connexin channels containing Cx43, Cx46, and Cx50 to signaling molecules and attempt to relate the observed differences in permeability to possible in vivo consequences that were revealed by studies of transgenic animals where these connexin genes have been manipulated. Taken together, these data suggest that differences in the permeability of individual connexin channels to larger solutes like 3′,5′-cyclic adenosine monophosphate (cAMP) and inositol 1,4,5-trisphosphate (IP3) could play a role in regulating epithelial cell division, differentiation, and homeostasis in organs like the ocular lens.
A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization
Authors: Agrillo Bruna , Proroga Yolande T. R. , Gogliettino Marta , Balestrieri Marco , Tatè Rosarita , Nicolais Luigi , Palmieri Gianna
Published: September 2020

Antimicrobial peptides (AMPs) are excellent candidates to fight multi-resistant pathogens worldwide and are considered promising bio-preservatives to control microbial spoilage through food processing. To date, designing de novo AMPs with high therapeutic indexes, low-cost synthesis, high resistance, and bioavailability, remains a challenge. In this study, a novel decapeptide, named RiLK1, was rationally designed starting from the sequence of the previously characterized AMP 1018-K6, with the aim of developing short peptides, and promoting higher selectivity over mammalian cells, antibacterial activity, and structural resistance under different salt, pH, and temperature conditions. Interestingly, RiLK1 displayed a broad-spectrum of bactericidal activity against Gram-positive and Gram-negative bacteria, including multidrug resistant clinical isolates of Salmonella species, with Minimal Bactericidal Concentration (MBC) values in low micromolar range, and it was effective even against two fungal pathogens with no evidence of cytotoxicity on human keratinocytes and fibroblasts. Moreover, RiLK1-activated polypropylene films were revealed to efficiently prevent the growth of microbial spoilage, possibly improving the shelf life of fresh food products. These results suggested that de novo designed peptide RiLK1 could be the first candidate for the development of a promising class of decameric and multitask antimicrobial agents to overcome drug-resistance phenomena.
Cannibalism in the Brown Marmorated Stink Bug Halyomorpha halys (Stål)
Authors: Papa Giulia , Negri Ilaria
Published: September 2020

The phytophagous brown marmorated stink bug Halyomorpha halys (Stål) is known to exhibit cannibalistic behaviour towards eggs. Here, we provide evidence of cannibalism among overwintering H. halys adults. Since diapausing individuals have high physiological demands for surviving long periods under stressful conditions, including the risk of depletion of metabolic reserves and desiccation, we assumed that nutritional and water requirements can be met by intraspecific predation. The role of aggregative behaviour in promoting cannibalism is also discussed. Given its evolutionary advantage, this trait should be maintained over generations and may be more widespread than previously considered in species that display aggregative behaviour during adverse seasons.
Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway
Authors: Wang Xue-yang , Ding Xin-yi , Chen Qian-ying , Zhang Kai-xiang , Zhao Chun-xiao , Tang Xu-dong , Wu Yang-chun , Li Mu-wang
Published: September 2020

Discovery of the anti-BmNPV (Bombyx mori nuclearpolyhedrovirus) silkworm strain suggests that some kind of antiviral molecular mechanism does exist but is still unclear. Apoptosis, as an innate part of the immune system, plays an important role in the response against pathogen infections and may be involved in the anti-BmNPV infection. Several candidate genes involved in the mitochondrial apoptosis pathway were identified from our previous study. Bombyx mori apoptosis protease-activating factor-1 (Bmapaf-1) was one of them, but the antiviral mechanism is still unclear. In this study, sequences of BmApaf-1 were characterized. It was found to contain a unique transposase_1 functional domain and share high CARD and NB-ARC domains with other species. Relatively high expression levels of Bmapaf-1 were found at key moments of embryonic development, metamorphosis, and reproductive development. Further, the significant difference in expression of Bmapaf-1 in different tissues following virus infection indicated its close relationship with BmNPV, which was further validated by RNAi and overexpression in BmN cells. Briefly, infection of budded virus with enhanced green fluorescent protein (BV-EGFP) was significantly inhibited at 72 h after overexpression of Bmapaf-1, which was confirmed after knockdown of Bmapaf-1 with siRNA. Moreover, the downstream genes of Bmapaf-1, including Bmnedd2-like caspase (BmNc) and Bmcaspase-1 (Bmcas-1), were upregulated after overexpression of Bmapaf-1 in BmN cells, which was consistent with the RNAi results. Furthermore, the phenomenon of Bmapaf-1 in response to BmNPV infection was determined to be related to apoptosis using the apoptosis inducer NSC348884 and inhibitor Z-DEVD-FMK. Therefore, Bmapaf-1 is involved in the response against BmNPV infection by the mitochondrial apoptosis pathway. This result provides valuable data for clarifying the anti-BmNPV mechanism of silkworms and breeding of resistant silkworm strains.
Mixed-Species Gardens Increase Monarch Oviposition without Increasing Top-Down Predation
Authors: Nestle Rebecca , Daniels Jaret C. , Dale Adam G.
Published: September 2020

Monarch butterfly populations have declined by over 80% in the last 20 years. Conservation efforts focus on the creation of milkweed habitats to mitigate this decline. Previous research has found monarchs lay more eggs per milkweed stem in urban gardens than natural habitats and recent work identified specific garden designs that make urban gardens more attractive to monarchs. Increasing plant diversity can reduce specialist insect herbivore colonization via bottom-up (e.g., plant) and top-down (e.g., predation) regulatory factors. Although this is beneficial for pest management efforts, it contradicts conservation efforts. In this study, we explored if adding multiple flowering species to garden-sized milkweed plantings affected monarch oviposition or top-down regulation of larvae. We compared monarch egg abundance, natural enemy abundance and richness, and biological control of monarch larvae in milkweed monocultures and milkweed mixed with four additional wildflower species. We found that monarchs laid 22% more eggs on sentinel milkweed plants in mixed-species plots with no effect of plant diversity on monarch survival. We also found higher natural enemy richness, wasp, and predatory bug abundance in the mixed-species plots and this did not translate to higher biological control rates. Our results provide more evidence that plant selection and habitat design are important for monarch conservation.
Diabetic Retinopathy: Mitochondria Caught in a Muddle of Homocysteine
Authors: Kowluru Renu A.
Published: September 2020

Diabetic retinopathy is one of the most feared complications of diabetes. In addition to the severity of hyperglycemia, systemic factors also play an important role in its development. Another risk factor in the development of diabetic retinopathy is elevated levels of homocysteine, a non-protein amino acid, and hyperglycemia and homocysteine are shown to produce synergistic detrimental effects on the vasculature. Hyperhomocysteinemia is associated with increased oxidative stress, and in the pathogenesis of diabetic retinopathy, oxidative stress-mitochondrial dysfunction precedes the development of histopathology characteristic of diabetic retinopathy. Furthermore, homocysteine biosynthesis from methionine forms S-adenosyl methionine (SAM), and SAM is a co-substrate of DNA methylation. In diabetes, DNA methylation machinery is activated, and mitochondrial DNA (mtDNA) and several genes associated with mitochondrial homeostasis undergo epigenetic modifications. Consequently, high homocysteine, by further affecting methylation of mtDNA and that of genes associated with mtDNA damage and biogenesis, does not give any break to the already damaged mitochondria, and the vicious cycle of free radicals continues. Thus, supplementation of sensible glycemic control with therapies targeting hyperhomocysteinemia could be valuable for diabetic patients to prevent/slow down the development of this sight-threatening disease.
Monoclonal and Bispecific Anti-BCMA Antibodies in Multiple Myeloma
Authors: Dalla Palma Benedetta , Marchica Valentina , Catarozzo Maria Teresa , Giuliani Nicola , Accardi Fabrizio
Published: September 2020

B-cell maturation antigen (BCMA), a member of the tumor necrosis factor receptor superfamily, is universally expressed by normal and neoplastic plasma cells and plays a critical role in the proliferation, survival and tumor progression in multiple myeloma (MM). B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) have been recognized as proliferation ligands for BCMA in the bone marrow microenvironment. Soluble BCMA levels in the serum correlates with disease phase and tumor burden and is a predictor of progression-free survival (PFS) and overall survival (OS). Recently, the introduction of new monoclonal antibodies against CD38 (Daratumumab and Isatuximab) and SLAM7 (Elotuzumab) has changed the therapeutic approach to MM, improving the response rate and the time to progression, both in newly diagnosed and refractory/relapsed patients. Among the surface antigens on MM cells, BCMA is a suitable target for the design of new antibody-based strategies. Experimental approaches targeting BCMA are currently being investigated and include antibody-drug conjugates (ADCs), bispecific antibodies (bsAbs) and genetically engineered T-cells with chimeric antigen receptors (CAR). In this review we summarize the more recent findings about BCMA biologic rationale as a therapeutic target and report the updated results of preclinical and clinical studies focused on ADCs and bsAbs targeting BCMA.
Power Management Circuits for Low-Power RF Energy Harvesters
Authors: Caselli Michele , Ronchi Marco , Boni Andrea
Published: September 2020

The paper describes the design and implementation of power management circuits for RF energy harvesters suitable for integration in wireless sensor nodes. In particular, we report the power management circuits used to provide the voltage supply of an integrated temperature sensor with analog-to-digital converter. A DC-DC boost converter is used to transfer efficiently the energy harvested from a generic radio-frequency rectifier into a charge reservoir, whereas a linear regulator scales the voltage supply to a suitable value for a sensing and conversion circuit. Implemented in a 65 nm CMOS technology, the power management system achieves a measured overall efficiency of 20%, with an available power of 4.5 μW at the DC-DC converter input. The system can sustain a temperature measurement rate of one sample/s with an RF input power of −28 dBm, making it compatible with the power levels available in generic outdoor environments.
Sound Transmission Loss of a Sandwich Plate with Adjustable Core Layer Thickness
Authors: Ehrig Tom , Dannemann Martin , Luft Ron , Adams Christian , Modler Niels , Kostka Pawel
Published: September 2020

Compressible Constrained Layer Damping (CCLD) is a novel, semi-active, lightweight-compatible solution for vibration mitigation based on the well-known constrained layer damping principle. The sandwich-like CCLD set-up consists of a base structure, a constraining plate, and a compressible open-cell foam core in between, enabling the adjustment of the structure’s vibration behaviour by changing the core compression using different actuation pressures. The aim of the contribution is to show to what degree, and in which frequency range the acoustic behaviour can be tuned using CCLD. Therefore, the sound transmission loss (TL), as an important vibro-acoustic index, is determined in an acoustic window test stand at different actuation pressures covering a frequency range from 0.5 to 5 kHz. The different actuation pressures applied cause a variation of the core layer thickness (from 0.9 d0 to 0.3 d0), but the resulting changes of the stiffness and damping of the overall structure have no significant influence on the TL up to approximately 1 kHz for the analysed CCLD design. Between 1 kHz and 5 kHz, however, the TL can be influenced considerably well by the actuation pressure applied, due to a damping-dominated behaviour around the critical frequency.
Preheated (Heat-Assisted) Clinching Process for Al/CFRP Cross-Tension Specimens
Authors: Lin Pai-Chen , Fang Jun-Chang , Lin Jia-Wei , Tran Xuan Van , Ching Yern-Chee
Published: September 2020

Effects of processing parameters on preheated (heat-assisted) clinching process to join aluminum alloy 5052-H32 (AA5052) and thermoplastic carbon-fiber-reinforced-plastic (TP-CFRP) sheets for cross-tension (CT) specimens were first studied. Preheating was critical since brittle TP-CFRP could be softened to avoid fracturing or cracking during clinching process. Four processing parameters, including punching force, die depth, heating mode, and heating temperature, were considered. Quasi-static tests and microscope observations were taken to evaluate AA5052/TP-CFRP clinch joints in CT specimens and determine appropriate processing parameters for fatigue tests. Finally, fatigue data and failure mode of clinch joints in CT specimens were obtained and discussed.
The LR-Type Fuzzy Multi-Objective Vendor Selection Problem in Supply Chain Management
Authors: Ali Irfan , Fügenschuh Armin , Gupta Srikant , Modibbo Umar Muhammad
Published: September 2020

Vendor selection is an established problem in supply chain management. It is regarded as a strategic resource by manufacturers, which must be managed efficiently. Any inappropriate selection of the vendors may lead to severe issues in the supply chain network. Hence, the desire to develop a model that minimizes the combination of transportation, deliveries, and ordering costs under uncertainty situation. In this paper, a multi-objective vendor selection problem under fuzzy environment is solved using a fuzzy goal programming approach. The vendor selection problem was modeled as a multi-objective problem, including three primary objectives of minimizing the transportation cost; the late deliveries; and the net ordering cost subject to constraints related to aggregate demand; vendor capacity; budget allocation; purchasing value; vendors’ quota; and quantity rejected. The proposed model input parameters are considered to be LR fuzzy numbers. The effectiveness of the model is illustrated with simulated data using R statistical package based on a real-life case study which was analyzed using LINGO 16.0 optimization software. The decision on the vendor’s quota allocation and selection under different degree of vagueness in the information was provided. The proposed model can address realistic vendor selection problem in the fuzzy environment and can serve as a useful tool for multi-criteria decision-making in supply chain management.
A Geometric Obstruction for CR-Slant Warped Products in a Nearly Cosymplectic Manifold
Authors: Uddin Siraj , Ullah M. Z.
Published: September 2020

In the early 20th century, B.-Y. Chen introduced the concept of CR-warped products and obtained several fundamental results, such as inequality for the length of second fundamental form. In this paper, we obtain B.-Y. Chen’s inequality for CR-slant warped products in nearly cosymplectic manifolds, which are the more general classes of manifolds. The equality case of this inequality is also investigated. Furthermore, the inequality is discussed for some important subclasses of CR-slant warped products.
Phosphatidylserine from Portunus trituberculatus Eggs Alleviates Insulin Resistance and Alters the Gut Microbiota in High-Fat-Diet-Fed Mice
Authors: Hu Shiwei , Du Mengyu , Su Laijin , Yang Huicheng
Published: September 2020

Portunus trituberculatus eggs contain phospholipids, whose components and bioactivity are unclear. Here, we investigated the fatty acid composition of phosphatidylserine from P. trituberculatus eggs (Pt-PS). Moreover, its effects on insulin resistance and gut microbiota were also evaluated in high-fat-diet-fed mice. Our results showed that Pt-PS accounted for 26.51% of phospholipids and contained abundant polyunsaturated fatty acids (more than 50% of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)). Animal experiments indicated that Pt-PS significantly decreased body weight and adipose weight gain, improved hyperglycemia and hyperinsulinemia, mitigated insulin resistance, and regulated circulatory cytokines. Pt-PS activated insulin receptor substrate 1 (IRS1) and increased the levels of IRS1-associated phosphatidylinositol 3-hydroxy kinase (PI3K), phosphorylated protein kinase B (Akt) protein, and plasma membrane glucose transporter 4 protein. Furthermore, Pt-PS modified the gut microbiota, inducing, especially, a dramatic decrease in the ratio of Firmicutes to Bacteroidetes at the phylum level, as well as a remarkable improvement in their subordinate categories. Pt-PS also reduced fecal lipopolysaccharide concentration and enhanced fecal acetate, propionate, and butyrate concentrations. Additionally, the effects of Pt-PS on alleviation of insulin resistance and regulation of intestinal bacteria were better than those of phosphatidylserine from soybean. These results suggest that Pt-PS mitigates insulin resistance by altering the gut microbiota. Therefore, Pt-PS may be developed as an effective food supplement for the inhibition of insulin resistance and the regulation of human gut health.
Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria
Authors: Yang Ziran , Hosokawa Hisaaki , Sadakane Takuya , Kuroda Masashi , Inoue Daisuke , Nishikawa Hiroshi , Ike Michihiko
Published: September 2020

Microbial antimonate (Sb(V)) reduction is a promising approach to remove Sb(V) from wastewater. However, current knowledge regarding microbial Sb(V) reduction is limited to strictly anaerobic conditions. This study was the first to isolate three facultative-anaerobic Sb(V)-reducing bacterial strains from the sludge collected from a wastewater treatment facility in an antimony products plant. Two of the isolated strains, designated Dechloromonas sp. AR-2 and Propionivibrio sp. AR-3, were characterized based on their Sb(V)-reducing abilities. When cultivated under anaerobic conditions with Sb(V) and acetate as the electron acceptor and donor, respectively, both strains could efficiently reduce 5.0 mM Sb(V), removing most of it from the water phase within 7 d. Along with Sb(V) reduction by the strains, white precipitates, which were likely amorphous Sb(OH)3 solids, were formed with a minor generation of soluble antimonite. Additionally, respiratory Sb(V) reduction by both strains occurred not only under anaerobic but also microaerobic conditions. It was suggested that Sb(V) reduction and the growth abilities of the strains under microaerobic conditions presented a substantial advantage of the use of strains AR-2 and AR-3 for practical applications to Sb(V)-containing wastewater treatment.
Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3
Authors: Goto Keiko , Yamaoka Yutaro , Khatun Hajera , Miyakawa Kei , Nishi Mayuko , Nagata Noriko , Yanaoka Toshikazu , Kimura Hirokazu , Ryo Akihide
Published: September 2020

Human parechovirus type 3 (HPeV3) is an etiologic agent of respiratory diseases, meningitis, and sepsis-like illness in both infants and adults. Monoclonal antibodies (mAbs) can be a promising diagnostic tool for antigenic diseases such as virus infection, as they offer a high specificity toward a specific viral antigen. However, to date, there is no specific mAb available for the diagnosis of HPeV3 infection. In this study, we developed and characterized mAbs specific for HPeV3 capsid protein VP0. We used cell-free, wheat germ-synthesized viral VP0 protein for immunizing BALB/c mice to generate hybridomas. From the resultant hybridoma clones, we selected nine clones producing mAbs reactive to the HPeV3-VP0 antigen, based on enzyme-linked immunosorbent assay (ELISA). Epitope mapping showed that these mAbs recognized three distinct domains in HPeV3 VP0. Six mAbs recognized HPeV3 specifically and the other three mAbs showed cross-reactivity with other HPeVs. Using the HPeV3-specific mAbs, we then developed an ELISA for viral antigen detection that could be reliably used for laboratory diagnosis of HPeV3. This ELISA system exhibited no cross-reactivity with other related viruses. Our newly developed mAbs would, thus, provide a useful set of tools for future research and ensure HPeV3-specific diagnosis.
Characterization of Slag Reprocessing Tailings-Based Geopolymers in Marine Environment
Authors: Wu Jie , Li Jing , Rao Feng , Yin Wanzhong
Published: September 2020

In this study, copper slag reprocessing tailings (CSRT) were synthesized into geopolymers with 40%, 50% and 60% metakaolin. The evolution of compressive strength and microstructures of CSRT-based geopolymers in a marine environment was investigated. Except for compressive strength measurement, the characterizations of X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) and scanning electron microscopy (SEM) were included. It was found that marine conditions changed the Si/Al ratio in the sodium-aluminosilicate-hydrate (N-A-S-H) gel backbone, promoted the geopolymerization process, led to more Q4(3Al), Q4(2Al) and Q4(1Al) gel formation and a higher compressive strength of the geopolymers. This provided a basis for the preparation of CSRT-based geopolymers into marine concrete.
The History of Transgressions during the Late Paleocene-Early Eocene in the Kuqa Depression, Tarim Basin: Constraints from C-O-S-Sr Isotopic Geochemistry
Authors: Xu Yang , Cao Yangtong , Liu Chenglin , Zhang Hua , Nie Xiao
Published: September 2020

The Tethys Sea extended into the Kuqa Depression from the Paleocene to the late Eocene and provided an abundant provenance for the deposition of evaporite sequences. Until now, detailed research on the history of transgressions during the late Paleocene-early Eocene in the Kuqa Depression has been limited. Therefore, in this study, we took the upper Paleocene Talak section and the lower Eocene Xiaokuzibai section in the western part of the Depression as the research objects and analyzed the petrology, the carbon and oxygen isotopes of carbonate rocks, and the sulfur and strontium isotopes of gypsum rocks to systematically study the above issues. The δ13C, δ18O and δ34S values of the upper Paleocene evaporite sequences were determined to be between 4.2‰ and 5.7‰, between −5.2‰ and 2.4‰, and between 16.5‰ and 17.9‰, respectively. The δ13C, δ18O, δ34S, and 87Sr/86Sr values of the lower Eocene evaporite sequences were determined to be between −6.9‰ and −2.0‰, between −9.0‰ and −4.5‰, between 10.5‰ and 17.0‰, and between 0.708642 and 0.709883, respectively. The analysis results show that the evaporite sequence of the upper Paleocene was formed by transgression. The deposition of the evaporite sequence changed from continental to marine deposition, and then gradually transitioned to continental during the Early Eocene. This paper is of great significance for reconstructing the history of transgressions in the Tethys tectonic realm during this period.
HPLC Fingerprint Analysis with the Antioxidant and Cytotoxic Activities of Selected Lichens Combined with the Chemometric Calculations
Authors: Hawrył Anna , Hawrył Mirosław , Hajnos-Stolarz Agnieszka , Abramek Jagoda , Bogucka-Kocka Anna , Komsta Łukasz
Published: September 2020

The aim of this study was to evaluate the ability of multivariate techniques to predict antioxidant and cytotoxic activity of the selected lichens from the chromatographic data. A simple and reproducible HPLC-DAD technique has been used to obtain the chromatographic fingerprint profiles. Reversed phase high performance liquid chromatography (RP-HPLC) linear gradient system with methanol, water and phosphoric acid (V) (pH 2.3) as the mobile phase was used (50 min). Principal Component Analysis (PCA) has been applied to the evaluation of the phytochemical similarity between studied samples, especially between the same species collected in various places of Poland (Cetraria islandica (L.) Ach., CI, Cladina mitis Sandst., CM, Hypogymnia physodes (L.) Nyl., HP). The ability to scavenge free radicals was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods and the total phenolic content was determined by Folin-Ciocalteu (F-C) test. In the case of DPPH % of inhibition was higher for selected species (Pseudevernia furfuracea (L.) Zopf, H. physodes in comparison to the literature data. The FRAP test showed that the H. physodes extract had higher ability to scavenge free radical in comparison to Cladonia furcata (Huds.) Schrader and Evernia prunastri (L.) Ach., whereas P. furfuracea extract showed higher ability than C. islandica. The high content of phenolics in P. furfuracea and H. physodes confirms their high antioxidant activity. The cytotoxic activity of studied extracts was tested by cell culture method using the human HL-60 / MX2 acute CKL-22 (CRL-2257) promyelocytic leukemia tumor cell line. The lowest values of IC50 [µg∙mL−1] were obtained for: H. physodes (HP1)—99.4; C. digitate—122.6; H. physodes (HP)—136.5, C. subulata—142.6; C. mitis—180.2.
Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family
Authors: Rolnik Agata , Kowalska Iwona , Soluch Agata , Stochmal Anna , Olas Beata
Published: September 2020

The aim of this study was to provide detailed insight into the chemical composition and activity of five cucurbit vegetable preparations (pumpkin, zucchini, cucumber, white and yellow pattypan squash), each containing various phytochemical compounds with potential use against oxidative stress induced by the hydroxyl radical donors in human plasma in vitro. We studied the antiradical capacity of vegetable preparations using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. As oxidative stress may induce changes in hemostasis, our aim included the determination of their effect on three selected hemostatic parameters of plasma, which are three coagulation times: PT (prothrombin time), APTT (activated partial thromboplastin time) and TT (thrombin time). However, none of used vegetable preparations changed APTT, PT or TT compared to the control. The phytochemical composition of the tested preparations was determined by UPLC-ESI-QTOF-MS. In our in vitro experiments, while all five tested preparations had antioxidant potential, the preparation from yellow pattypan squash showed the strongest potential. All cucurbit vegetable preparations inhibited lipid peroxidation. Only zucchini did not have an effect on protein carbonylation and only yellow pattypan squash inhibited thiol oxidation. The antioxidant activity of cucurbits appears to have triggered significant interest in multiple applications, including CVDs (cardiovascular diseases) associated with oxidative stress, which can be treated by supplementation based on these vegetables.