bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Tetrahydrolipstatin on Glioblastoma in Mice: MRI-Based Morphologic and Texture Analysis Correlated with Histopathology and Immunochemistry Findings—A Pilot Study
Authors: Wagner Sabine , Ewald Christian , Freitag Diana , Herrmann Karl-Heinz , Koch Arend , Bauer Johannes , Vogl Thomas J. , Kemmling André , Gufler Hubert
Published: April 2024

Background: This study aimed to investigate the effects of tetrahydrolipstatin (orlistat) on heterotopic glioblastoma in mice by applying MRI and correlating the results with histopathology and immunochemistry. Methods: Human glioblastoma cells were injected subcutaneously into the groins of immunodeficient mice. After tumor growth of >150 mm3, the animals were assigned into a treatment group (n = 6), which received daily intraperitoneal injections of orlistat, and a control group (n = 7). MRI was performed at the time of randomization and before euthanizing the animals. Tumor volumes were calculated, and signal intensities were analyzed. The internal tumor structure was evaluated visually and with texture analysis. Western blotting and protein expression analysis were performed. Results: At histology, all tumors showed high mitotic and proliferative activity (Ki67 ≥ 10%). Reduced fatty acid synthetase expression was measured in the orlistat group (p < 0.05). Based on the results of morphologic MRI-based analysis, tumor growth remained concentric in the control group and changed to eccentric in the treatment group (p < 0.05). The largest area under the receiver operating curve of the predictors derived from the texture analysis of T2w images was for wavelet transform parameters WavEnHL_s3 and WavEnLH_s4 at 0.96 and 1.00, respectively. Conclusions: Orlistat showed effects on heterotopically implanted glioblastoma multiforme in MRI studies of mice based on morphologic and texture analysis.
Correlation of Molecular Status with Preoperative Olfactory Function in Olfactory Groove Meningioma
Authors: Podlesek Dino , Beyer Friederike , Alkhatib Majd , Daubner Dirk , Hijazi Mido Max , Juratli Jerry Hadi , Weise Susanne , Eyüpoglu Ilker Y. , Schackert Gabriele , Juratli Tareq A. , Hummel Thomas
Published: April 2024

Purpose: The study aims to examine the possible correlation between genomic alterations and preoperative olfactory function in patients with olfactory groove meningioma (OGM), due to the frequent presence of olfactory impairment. Methods: We utilised next-generation sequencing to analyse samples from 22 individuals with OGM in order to detect driver mutations. Tumour morphology was assessed using preoperative imaging, whereas olfactory function was examined using Sniffin’ Sticks. Results: In a study of 22 OGM patients, mutations were as follows: 10 with SMO/SUFU, 7 with AKT1, and 5 as wild type. Planum sphenoidale hyperostosis (PSH) was present in 75% of patients, showing significant variation by mutation (p = 0.048). Tumour volumes, averaging 25 cm3, significantly differed among groups. PSH negatively impacted olfaction, notably affecting odour threshold, discrimination, identification, and global olfactory performance score (TDI) (p values ranging from <0.001 to 0.003). Perifocal oedema was associated with lower TDI (p = 0.009) and altered threshold scores (p = 0.038). Age over 65 and female gender were linked to lower thresholds and discrimination scores (p = 0.037 and p = 0.019). Conclusion: The study highlights PSH and perifocal oedema’s significant effect on olfactory function in OGM patients but finds no link between olfactory impairment and tumour mutations, possibly due to the small sample size. This suggests that age and gender affect olfactory impairment. Additional research with a larger group of participants is needed to explore the impact of OGM driver mutations on olfactory performance.
Immune Therapies in AL Amyloidosis—A Glimpse to the Future
Authors: Haran Arnon , Vaxman Iuliana , Gatt Moshe E. , Lebel Eyal
Published: April 2024

Light-chain (AL) amyloidosis is a rare plasma cell disorder characterized by the deposition of misfolded immunoglobulin light chains in target organs, leading to multi-organ dysfunction. Treatment approaches have historically mirrored but lagged behind those of multiple myeloma (MM). Recent advancements in MM immunotherapy are gradually being evaluated and adopted in AL amyloidosis. This review explores the current state of immunotherapeutic strategies in AL amyloidosis, including monoclonal antibodies, antibody–drug conjugates, bispecific antibodies, and chimeric antigen receptor T-cell therapy. We discuss the unique challenges and prospects of these therapies in AL amyloidosis, including the exposure of frail AL amyloidosis patients to immune-mediated toxicities such as cytokine release syndrome (CRS) and immune effector-cell-associated neurotoxicity syndrome (ICANS), as well as their efficacy in promoting rapid and deep hematologic responses. Furthermore, we highlight the need for international initiatives and compassionate programs to provide access to these promising therapies and address critical unmet needs in AL amyloidosis management. Finally, we discuss future directions, including optimizing treatment sequencing and mitigating toxicities, to improve outcomes for AL amyloidosis patients.
Patients with Advanced or Metastasised Non-Small-Cell Lung Cancer with Viscum album L. Therapy in Addition to PD-1/PD-L1 Blockade: A Real-World Data Study
Authors: Schad Friedemann , Thronicke Anja , Hofheinz Ralf-Dieter , Matthes Harald , Grah Christian
Published: April 2024

Immunotherapy with PD-1/PD-L1 inhibitors has significantly improved the survival rates of patients with metastatic non-small-cell lung cancer (NSCLC). Results of a real-world data study investigating add-on VA (Viscum album L.) to chemotherapy have shown an association with the improved overall survival of patients with NSCLC. We sought to investigate whether the addition of VA to PD-1/PD-L1 inhibitors in patients with advanced or metastasised NSCLC would have an additional survival benefit. In the present real-world data study, we enrolled patients from the accredited national registry, Network Oncology, with advanced or metastasised NSCLC. The reporting of data was performed in accordance with the ESMO-GROW criteria for the optimal reporting of oncological real-world evidence (RWE) studies. Overall survival was compared between patients receiving PD-1/PD-L1 inhibitor therapy (control, CTRL group) versus the combination of anti-PD-1/PD-L1 therapy and VA (combination, COMB group). An adjusted multivariate Cox proportional hazard analysis was performed to investigate variables associated with survival. From 31 July 2015 to 9 May 2023, 415 patients with a median age of 68 years and a male/female ratio of 1.2 were treated with anti-PD-1/PD-L1 therapy with or without add-on VA. Survival analyses included 222 (53.5%) patients within the CRTL group and 193 (46.5%) in the COMB group. Patients in the COMB group revealed a median survival of 13.8 months and patients in the CRTL group a median survival of 6.8 months (adjusted hazard ratio, aHR: 0.60, 95% CI: 0.43–0.85, p = 0.004) after adjustment for age, gender, tumour stage, BMI, ECOG status, oncological treatment, and PD-L1 tumour proportion score. A reduction in the adjusted hazard of death by 56% was seen with the addition of VA (aHR 0.44, 95% CI: 0.26–0.74, p = 0.002) in patients with PD-L1-positive tumours (tumour proportion score > 1%) treated with first-line anti-PD-1/PD-L1 therapy. Our findings suggest that add-on VA correlates with improved survival in patients with advanced or metastasised NSCLC who were treated with PD-1/PD-L1 inhibitors irrespective of age, gender, tumour stage, or oncological treatment. The underlying mechanisms may include the synergistic modulation of the immune response. A limitation of this study is the observational non-randomised study design, which only allows limited conclusions to be drawn and prospective randomised trials are warranted.
An Overview of the Spices Used for the Prevention and Potential Treatment of Gastric Cancer
Authors: Kostelecka Katarzyna , Bryliński Łukasz , Komar Olga , Michalczyk Justyna , Miłosz Agata , Biłogras Jan , Woliński Filip , Forma Alicja , Baj Jacek
Published: April 2024

Gastric cancer (GC) ranks third in terms of cancer-related deaths and is the fifth most commonly diagnosed type of cancer. Its risk factors include Helicobacter pylori infection, Epstein–Barr virus infection, the consumption of broiled and charbroiled animal meats, salt-preserved and smoke-enhanced foods, alcohol drinking, tobacco smoking, exposure to ionizing radiation, and positive family history. The limited effectiveness of conventional therapies and the widespread risk factors of GC encourage the search for new methods of treatment and prevention. In the quest for cheap and commonly available medications, numerous studies focus on herbal medicine, traditional brews, and spices. In this review, we outline the potential use of spices, including turmeric, ginger, garlic, black cumin, chili pepper, saffron, black pepper, rosemary, galangal, coriander, wasabi, cinnamon, oregano, cardamom, fenugreek, caraway, clove, dill, thyme, Piper sarmentosum, basil, as well as the compounds they contain, in the prevention and treatment of GC. We present the potential molecular mechanisms responsible for the effectivity of a given seasoning substance and their impact on GC cells. We discuss their potential effects on proliferation, apoptosis, and migration. For most of the spices discussed, we also outline the unavailability and side effects of their use.
Preparation of Conductive Asphalt Concrete Based on the Action Mechanism of Conductive Phase Materials
Authors: Li Xiujun , Zhang Zhipeng , Zhang Heng , Ma Huaiyu , Shi Fangzhi
Published: April 2024

Carbon fiber powder (CFP) was first applied to conductive asphalt concrete as a conductive phase material, but its action mechanism has not been clarified. In this paper, atomic force microscopy (AFM) and molecular dynamics (MDs) simulation are used to study the carbon fiber powder mechanism of action, guide the preparation of conductive asphalt concrete, and study the electrothermal properties of conductive asphalt concrete. The results show that carbon fiber powder weakens the adhesion property of asphalt mastic, and this weakening further strengthens in the water–temperature coupling, so water stability and conductivity are used as evaluation indicators to determine that the optimal content of carbon fiber powder is 2.0% and that the optimal content of carbon fibers (CFs) is 0.4%. Carbon fiber–carbon fiber powder conductive asphalt concrete with a resistivity of 0.98 Ω·m was finally prepared. In the temperature rise test of the Marshall specimen and rutting slab, its warming effect is obvious, and the heat transformation rate is more than 75%, so it has a very good ability to melt snow and ice.
Data-Driven Enterprise Architecture for Pharmaceutical R&D
Authors: Uzhakova (née Sabirzyanova) Nailya , Fischer Stefan
Published: April 2024

This paper addresses the research gap in the realm of data-driven transformation by leveraging the Resource-Based View (RBV) theory and the dynamic capabilities concept to the contours of a data-driven enterprise. It confronts the limitations of conventional digital and data transformation programs, which often prioritize technological enhancements over crucial organizational and cultural shifts. Proposing a more holistic perspective, the Data-Driven Enterprise Architecture Framework (DDA) is introduced, emphasizing the domain decomposition and productization of an architecture, distributed ownership, and federated governance, while ensuring the continuous harmonization of data, application, and business architecture. A case study featuring a leading pharmaceutical company illustrates the practical implementation of the DDA framework as a pillar of their Digital Transformation Strategy. By integrating scalable and distributed data architecture into the overarching Enterprise Architecture landscape, the company has initiated their data-driven transformation journey, showcased through their initial and very early results. This research not only offers valuable insights for pharmaceutical organizations navigating the complexities of data-driven transformations, but also addresses a research gap in the field.
The Modelling of the Evapotranspiration Portion of the Water Footprint: A Global Sensitivity Analysis in the Brazilian Serra Gaúcha
Authors: Platt Gustavo Mendes , Nunes Vinícius Kuczynski , Martins Paulo Roberto , Corrêa Ricardo Gonçalves de Faria , Oliveira Francisco Bruno Souza
Published: April 2024

Water footprints have been widely used to illustrate the consumption of water in many situations, for instance, in products, processes, or regions of interest. In this work, we analyzed—using a sensitivity analysis approach—the effect of some variables in the calculation of the water footprint in the viticulture in the Brazilian Serra Gaúcha (the major producing region of Brazilian wine). The classical Penman–Monteith model for evapotransporation was considered, with uncertainties in some parameters (dead mulch covering a fraction of the vineyard, maximum temperatures for some months, the altitudes and latitudes of the site). A sensitivity analysis was conducted using the SAFE toolbox under Octave framework. The results indicated that the the portion of the water footprint corresponding to evapotranspiration is more sensitive to the values of the mulch-covered fraction and the altitude of the site in comparison with the latitude and the maximum temperatures.
Optimization of Electrical and Thermal Storage in a High School Building in Central Greece
Authors: Roumpakias Elias , Zogou Olympia , Stamatellou Antiopi-Malvina
Published: April 2024

Nearly zero-emission buildings (nZEBs) are increasingly being constructed in Europe. There are also incentives to refurbish older buildings and transform them into nZEBs. However, permission is not always granted for their connection to the grid to infuse surplus photovoltaic electricity due to the grid being overloaded with a large number of renewables. In this study, the case of a refurbished school building in Central Greece is examined. After refurbishing it, a significant amount of photovoltaic electricity surplus is observed during the summer and neutral months, which cannot be exported to the grid. The absence of an adequate battery storage capacity resulted in the rejection of an application for exporting the school’s surplus to the network and the photovoltaic installation staying idle. An alternative approach is proposed in this work, involving a shift in the export of the photovoltaic electricity surplus to the evening hours, in order for the school to be granted permission to export it to the network. To this end, an optimal battery storage size is sought by employing a building energy system simulation. The mode of operation of the battery designed for this application is set to discharge daily, in order to export the electricity surplus in the afternoon hours to the evening hours, when it is favorable for the network. Additionally, the optimal size of the thermal energy storage of the heating system is studied to further improve its energy efficiency. Our battery and storage tank size optimization study shows that a significant battery capacity is required, with 12 kWh/kWp photovoltaic panels being recommended for installation. The ever-decreasing cost of battery installations results in the net present value (NPV) of the additional investment for the battery installation becoming positive. The solution proposed forms an alternative path to further increase the penetration of renewables in saturated networks in Greece by optimizing battery storage capacity.
Distribution Network Reconfiguration Based on an Improved Arithmetic Optimization Algorithm
Authors: Jia Hui , Zhu Xueling , Cao Wensi
Published: April 2024

Aiming to address the defects of the arithmetic optimization algorithm (AOA), such as easy fall into local optimums and slow convergence speed during the search process, an improved arithmetic optimization algorithm (IAOA) is proposed and applied to the study of distribution network reconfiguration. Firstly, a reconfiguration model is established to reduce network loss, and a cosine control factor is introduced to reconfigure the math optimization accelerated (MOA) function to coordinate the algorithm’s global exploration and local exploitation capabilities. Subsequently, a reverse differential evolution strategy is introduced to improve the overall diversity of the population and Weibull mutation is performed on the better-adapted individuals generated in each iteration to ensure the quality of the optimal individuals generated in each iteration and strengthen the algorithm’s ability to approach the optimal solution. The performance of the improved algorithm is also tested using eight basis functions. Finally, simulation analysis is carried out by taking the IEEE33 and IEEE69 node systems and a real power distribution system as examples; the results show that the proposed algorithm can help to reconfigure the system quickly, and the system node voltages and network losses were significantly improved after the reconfiguration.
The Study of Multi-Terminal DC Systems in an Offshore Wind Environment: A Focus on Cable Ripple Analysis
Authors: Rong Xiaoyun , Shek Jonathan K. H. , Macpherson D. Ewen , Mawby Phil
Published: April 2024

This paper studies the THD and AC losses on the DC cables of offshore wind farm-based multi-terminal HVDC systems when they extract and deliver power from and to more than one connection point. In the paper, the study of a full system PLECS + Simulink model with two branches, including a wind resource, a wind turbine, a Permanent Magnet Synchronous Generator (PMSG), a Pulse Width Modulation (PWM) rectifier, a Single Active Bridge (SAB) DC–DC converter, an Input Parallel Output Series (IPOS) DC–DC converter, HVDC cables, and a simplified onshore system, is presented. It focuses on the investigation of the output ripple content of multiple DC–DC converters on DC cables under different wind conditions with different voltage and power ratings. The importance of the study is providing a general understanding of the operation of the innovative offshore wind farm-based DC system, as well as the interaction between different DC–DC converters and their influence on cable ripple content under different situations.
Prognostic Value of “Basal-like” Morphology, Tumor-Infiltrating Lymphocytes and Multi-MAGE-A Expression in Triple-Negative Breast Cancer
Authors: Čeprnja Toni , Tomić Snježana , Perić Balja Melita , Marušić Zlatko , Blažićević Valerija , Spagnoli Giulio Cesare , Juretić Antonio , Čapkun Vesna , Vuger Ana Tečić , Pogorelić Zenon , Mrklić Ivana
Published: April 2024

“Basal-like” (BL) morphology and the expression of cancer testis antigens (CTA) in breast cancer still have unclear prognostic significance. The aim of our research was to explore correlations of the morphological characteristics and tumor microenvironment in triple-negative breast carcinomas (TNBCs) with multi-MAGE-A CTA expression and to determine their prognostic significance. Clinical records of breast cancer patients who underwent surgery between January 2017 and December 2018 in four major Croatian clinical centers were analyzed. A total of 97 non-metastatic TNBCs with available tissue samples and treatment information were identified. Cancer tissue sections were additionally stained with programmed death-ligand 1 (PD-L1) Ventana (SP142) and multi-MAGE-A (mAb 57B). BL morphology was detected in 47 (49%) TNBCs and was associated with a higher Ki-67 proliferation index and histologic grade. Expression of multi-MAGE-A was observed in 77 (79%) TNBCs and was significantly associated with BL morphology. Lymphocyte-predominant breast cancer (LPBC) status was detected in 11 cases (11.3%) and significantly correlated with the Ki-67 proliferation index, increased number of intratumoral lymphocytes (itTIL), and PD-L1 expression. No impact of BL morphology, multi-MAGE-A expression, histologic type, or LPBC status on disease-free survival was observed. Our data suggest that tumor morphology could help identify patients with potential benefits from CTA-targeting immunotherapy.
Variation in Shrimp Allergens: Place of Origin Effects on Food Safety Assessment
Authors: Dorney Ryley D. , Johnston Elecia B. , Karnaneedi Shaymaviswanathan , Ruethers Thimo , Kamath Sandip D. , Gopi Karthik , Mazumder Debashish , Sammut Jesmond , Jerry Dean , Williamson Nicholas A. , Nie Shuai , Lopata Andreas L.
Published: April 2024

Due to the widespread use of shellfish ingredients in food products, accurate food labelling is urgently needed for consumers with shellfish allergies. Most crustacean allergen detection systems target the immunorecognition of the allergenic protein tropomyosin. However, this mode of detection may be affected by an origin-dependent protein composition. This study determined if the geographic location of capture, or aquaculture, influenced the allergenic protein profiles of Black Tiger Shrimp (Penaeus monodon), one of the most farmed and consumed shrimp species worldwide. Protein composition was analysed in shrimp from nine different locations in the Asia–Pacific by SDS-PAGE, immunoblotting, and mass spectrometry. Ten of the twelve known shrimp allergens were detected, but with considerable differences between locations. Sarcoplasmic calcium-binding protein, myosin light chain, and tropomyosin were the most abundant allergens in all locations. Hemocyanin-specific antibodies could identify up to six different isoforms, depending on the location of origin. Similarly, tropomyosin abundance varied by up to 13 times between locations. These findings suggest that allergen abundance may be related to shrimp origin and, thus, shrimp origin might directly impact the readout of commercial crustacean allergen detection kits, most of which target tropomyosin, and this should be considered in food safety assessments.
Comparison of GFAP and UCH-L1 Measurements Using Two Automated Immunoassays (i-STAT® and Alinity®) for the Management of Patients with Mild Traumatic Brain Injury: Preliminary Results from a French Single-Center Approach
Authors: Oris Charlotte , Khatib-Chahidi Clara , Pereira Bruno , Bailly Defrance Valentin , Bouvier Damien , Sapin Vincent
Published: April 2024

The measurement of blood glial fibrillary acidic protein (GFAP) and ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) may assist in the management of mild traumatic brain injury (mTBI). This study aims to compare GFAP and UCH-L1 values measured using a handheld device with those measured using a core laboratory platform. We enrolled 230 mTBI patients at intermediate risk of complications. Following French guidelines, a negative S100B value permits the patient to be discharged without a computed tomography scan. Plasma GFAP and UCH-L1 levels were retrospectively measured using i-STAT® and Alinity® i analyzers in patients managed within 12 h post-trauma. Our analysis indicates a strong correlation of biomarker measurements between the two analyzers. Cohen’s kappa coefficients and Lin’s concordance coefficients were both ≥0.7, while Spearman’s correlation coefficient was 0.94 for GFAP and 0.90 for UCH-L1. Additionally, the diagnostic performance in identifying an intracranial lesion was not significantly different between the two analyzers, with a sensitivity of 100% and specificity of approximately 30%. GFAP and UCH-L1 levels measured using Abbott’s i-STAT® and Alinity® i platform assays are highly correlated both analytically and clinically in a cohort of 230 patients managed for mTBI according to French guidelines.
The Integration of Transcriptome and Metabolome Analyses Provides Insights into the Determinants of the Wood Properties in Toona ciliata
Authors: Wang Zhi , Wu Jinsong , Kong Weijia , Zhou Yu , Ye Chunyi , Yuan Qianyun , Zhang Yongjia , Li Pei
Published: April 2024

Toona ciliata, also known as Chinese mahogany, is a high-quality and fast-growing wood species with a high economic value. The wood properties of T. ciliata of different provenances vary significantly. In this study, we conducted comprehensive transcriptome and metabolome analyses of red and non-red T. ciliata wood cores of different provenances to compare their wood properties and explore the differential metabolites and genes that govern the variation in their wood properties. Through combined analyses, three differential genes and two metabolites were identified that are possibly related to lignin synthesis. The lignin content in wood cores from T. ciliata of different provenances shows significant variation following systematic measurement and comparisons. The gene Tci09G002190, one of the three differential genes, was identified as a member of the CAD (Cinnamyl alcohol dehydrogenase) gene family of T. ciliata, which is associated with lignin synthesis. Our data provide insights into the determinants of the wood properties in T. ciliata, providing a solid foundation for research into the subsequent mechanisms of the formation of T. ciliata wood.
Contributions of Non-Neuronal Cholinergic Systems to the Regulation of Immune Cell Function, Highlighting the Role of α7 Nicotinic Acetylcholine Receptors
Authors: Kawashima Koichiro , Mashimo Masato , Nomura Atsuo , Fujii Takeshi
Published: April 2024

Loewi’s discovery of acetylcholine (ACh) release from the frog vagus nerve and the discovery by Dale and Dudley of ACh in ox spleen led to the demonstration of chemical transmission of nerve impulses. ACh is now well-known to function as a neurotransmitter. However, advances in the techniques for ACh detection have led to its discovery in many lifeforms lacking a nervous system, including eubacteria, archaea, fungi, and plants. Notably, mRNAs encoding choline acetyltransferase and muscarinic and nicotinic ACh receptors (nAChRs) have been found in uninnervated mammalian cells, including immune cells, keratinocytes, vascular endothelial cells, cardiac myocytes, respiratory, and digestive epithelial cells. It thus appears that non-neuronal cholinergic systems are expressed in a variety of mammalian cells, and that ACh should now be recognized not only as a neurotransmitter, but also as a local regulator of non-neuronal cholinergic systems. Here, we discuss the role of non-neuronal cholinergic systems, with a focus on immune cells. A current focus of much research on non-neuronal cholinergic systems in immune cells is α7 nAChRs, as these receptors expressed on macrophages and T cells are involved in regulating inflammatory and immune responses. This makes α7 nAChRs an attractive potential therapeutic target.
Metal–Semiconductor Behavior along the Line of Stacking Order Change in Gated Multilayer Graphene
Authors: Jaskólski Włodzimierz
Published: April 2024

We investigated gated multilayer graphene with stacking order changes along the armchair direction. We consider that some layers cracked to release shear strain at the stacking domain wall. The energy cones of graphene overlap along the corresponding direction in the k-space, so the topological gapless states from different valleys also overlap. However, these states strongly interact and split due to atomic-scale defects caused by the broken layers, yielding an effective energy gap. We find that for some gate voltages, the gap states cross and the metallic behavior along the stacking domain wall can be restored. In particular cases, a flat band appears at the Fermi energy. We show that for small variations in the gate voltage, the charge occupying this band oscillates between the outer layers.
Consumption of Dietary Supplements among Working-Age Residents of Lithuania in the Period from 2021 to 2023
Authors: Arlauskas Rokas , Austys Donatas , Dobrovolskij Valerij , Stukas Rimantas
Published: April 2024

Background and Objectives: The aim of this study was to assess the consumption of dietary supplements (DS) among working-age residents of Lithuania from 2021 to 2023 with respect to social and demographic factors and an assessment of personal health. Materials and Methods: Using stratified sampling techniques, this study included three samples of working-age residents (1600 each year, 4800 total). Three surveys were conducted, the distribution of the respondents between groups was compared using the χ2 test. Results: The consumption of DS significantly differed each year and accounted for 78.1%, 71.6%, and 72.7% of the respondents, respectively (p < 0.05). In 2022, the prevalence of the consumption of DS was lower in the majority of social and demographic groups (p < 0.05). In 2023, it was higher among females, younger residents, and those from larger families, who suffered from COVID-19 (p < 0.05). Despite similar changes found in the consumption of DS among those who negatively assessed their health, this group showed more prevalent consumption of DS among residents with non-university education, unemployed respondents, and those with lower income (p < 0.05). Conclusions: Despite a significantly lower prevalence in the consumption of DS in 2022, it was higher again in 2023. The assessment of personal health shows different habits in the consumption of DS.
Association between Antenatal Vaginal Bleeding and Adverse Perinatal Outcomes in Placenta Accreta Spectrum
Authors: Mulhall J. Connor , Ireland Kayla E. , Byrne John J. , Ramsey Patrick S. , McCann Georgia A. , Munoz Jessian L.
Published: April 2024

Background and Objectives: Placenta accreta spectrum (PAS) disorders are placental conditions associated with significant maternal morbidity and mortality. While antenatal vaginal bleeding in the setting of PAS is common, the implications of this on overall outcomes remain unknown. Our primary objective was to identify the implications of antenatal vaginal bleeding in the setting of suspected PAS on both maternal and fetal outcomes. Materials and Methods: We performed a case-control study of patients referred to our PAS center of excellence delivered by cesarean hysterectomy from 2012 to 2022. Subsequently, antenatal vaginal bleeding episodes were quantified, and components of maternal morbidity were assessed. A maternal composite of surgical morbidity was utilized, comprised of blood loss ≥ 2 L, transfusion ≥ 4 units of blood, intensive care unit (ICU) admission, and post-operative length of stay ≥ 4 days. Results: During the time period, 135 cases of confirmed PAS were managed by cesarean hysterectomy. A total of 61/135 (45.2%) had at least one episode of bleeding antenatally, and 36 (59%) of these had two or more bleeding episodes. Increasing episodes of antenatal vaginal bleeding were associated with emergent delivery (p < 0.01), delivery at an earlier gestational age (35 vs. 34 vs. 33 weeks, p < 0.01), and increased composite maternal morbidity (76, 84, and 94%, p = 0.03). Conclusions: Antenatal vaginal bleeding in the setting of PAS is associated with increased emergent deliveries, earlier gestational ages, and maternal composite morbidity. This important antenatal event may aid in not only counseling patients but also in the coordination of multidisciplinary teams caring for these complex patients.
The Relationship between Neutrophil–Lymphocyte Ratios with Nutritional Status, Risk of Nutritional Indices, Prognostic Nutritional Indices and Morbidity in Patients with Ischemic Stroke
Authors: Misirlioglu Naile Fevziye , Uzun Nedim , Ozen Gulenay Defne , Çalik Mustafa , Altinbilek Ertugrul , Sutasir Necmettin , Baykara Sayili Sena , Uzun Hafize
Published: April 2024

Background: In recent years, whole blood parameters and derivatives have been used as prognostic criteria in the course of various diseases. The aim of this study was to evaluate the relationship between parameters such as the neutrophil–lymphocyte ratio (NLR), the systemic immune-inflammation index (SII), the prognostic nutritional index (PNI), controlling nutritional status (CONUT) score, nutritional risk index (NRI) and immunonutrition status and disease activity in patients with ischemic stroke of the small-vessel, large-vessel and other etiologies. Methods: We retrospectively evaluated the records of 1454 consecutive ischemic stroke patients hospitalized in the emergency department of Gaziosmanpasa Education and Research Hospital from 2019 to 2023. Results: Of the 1350 patients with ischemic stroke included in the study, 58.8% had small-vessel disease, 29.3% had large-vessel disease and 11.9% had other etiologies. There was a significant difference between the three etiology groups for PNI and CONUT. The mean of PNI was 47.30 ± 8.06 in the other etiology group, 37.25 ± 7.23 in the small-vessel group, and 34.78 ± 8.16 in the large-vessel disease group. The mean of CONUT was 5.49 ± 1.20 in the small-vessel group, 5.12 ± 1.46 in the large-vessel group and 4.22 ± 1.11 in the other etiology group. In addition, CONUT and PNI were also found to be independent risk factors for mortality. A negative significant correlation was observed between PNI and NLR (r: −0.692), SII (r: −0.591), and CONUT (r: −0.511). Significant correlations were observed between CONUT and NLR (r: 0.402), SII (r: 0.312). Conclusions: PNI, CONUT and NRI were found as more accurate prognostic indicators of nutritional status in patients with ischemic stroke. NLR and SII may be important predictive markers in the course and prognosis of stroke.
Exploring the Relationship between Mediterranean Diet Adherence and Subjective Well-Being among Greek and Cypriot Adults
Authors: Deligiannidou Georgia-Eirini , Philippou Elena , Vasiari Eirini , de Andrade Vanda Lopes , Massaro Marika , Chervenkov Mihail , Ivanova Teodora , Jorge Rui , Dimitrova Dessislava , Ruskovska Tatjana , Miloseva Lence , Maksimova Viktorija , Smilkov Katarina , Gjorgieva Ackova Darinka , García-Conesa María-Teresa , Pinto Paula , Kontogiorgis Christos A.
Published: April 2024

Associations between subjective well-being (SWB) and dietary habits, employment status, and habitual activities are increasingly capturing the focus of researchers as well as policymakers worldwide. This study aimed to explore these associations in a sample of the population in Greece and Cyprus via an online survey. In total, 936 questionnaires (470: Cyprus, 466: Greece) were analyzed to study the associations between the Mediterranean Diet (MD) (using the 14-item MEDAS score, (14-MEDAS)), subjective well-being (SWB), and several socioeconomic factors. Key remarks of this survey highlight the positive impact of MD adherence on some well-being items. Namely, statistically significant differences were found on the following items: Satisfied with life (p < 0.001), Life worthwhile (p < 0.001), Feeling happy (p < 0.001), worried (p = 0.005), and depressed (p = 0.001), when comparing Low MD adherence (14-MEDAS < 5) to High MD adherence (14-MEDAS > 10). Other lifestyle habits such as spending time with friends and family, spending time in nature, and habitual physical activity were associated with aspects of SWB such as Life satisfaction, Life worthwhile, Feeling happy, and energetic. The findings support adherence to the MD, since it is associated with higher life satisfaction and self-reported happiness in this sample and should be considered when developing health policies on well-being.
IL-10 and IL-1β Serum Levels, Genetic Variants, and Metabolic Syndrome: Insights into Older Adults’ Clinical Characteristics
Authors: Freitas Renata de Souza , de Souza Silva Calliandra Maria , Ferreira Fratelli Caroline , Ramos de Lima Luciano , Morato Stival Marina , Schwerz Funghetto Silvana , Rodrigues da Silva Izabel Cristina , Vieira de Andrade Rosângela
Published: April 2024

Populational aging is marked by chronic noncommunicable diseases, such as metabolic syndrome (MetS). IL-10 and IL-1β are pleiotropic cytokines with multiple biological effects linked to metabolic disorders. This cross-sectional study assessed 193 participants’ IL-10 and IL-1β serum levels regarding their role in developing MetS, clinical characteristics, and their IL1B rs1143627 and IL10 rs1800890 variants’ genotype frequencies in a population over 60. IL-10 levels correlated weakly with HDL levels and fat mass and inversely with triglycerides, glucose, glycated hemoglobin, and estimated average blood glucose levels. IL-10 levels were also indirectly influenced by the patient’s T2DM duration, lean mass amount, and bone mineral content. Participants with altered HDL, elevated serum glucose, raised HbA1c levels, or those over 80 had reduced serum IL-10 levels compared to those with normal levels or other age groups, respectively. Women also had higher serum IL-10 levels than men. Dissimilarly, IL-1β levels correlated directly only with the number of total leukocytes and segmented neutrophils, showing only significant variations with self-reported alcohol consumption. Our study also found that those with the IL10 AA genotype (lower IL-10 levels) had a significantly higher risk of developing MetS. These findings may help direct future research and more targeted therapeutic approaches in older adults.
Dietary Quality Evidenced by the Healthy Eating Index and Cardiovascular Disease Risk Factors in Kuwaiti Schoolchildren
Authors: Al-Farhan Abdulaziz , Weatherspoon Lorraine , Pfeiffer Karin , Li Wei , Carlson Joseph
Published: April 2024

Background: Poor dietary quality is associated with adiposity and other risks of cardiovascular disease (CVD) in children. In Kuwait, although children’s food choices are a concern, no studies have evaluated dietary quality relative to the risk of CVD in Kuwaiti schoolchildren. This study hypothesized that dietary quality using the Healthy Eating Index (HEI) is associated with CVD risk factors in children and that there are associated sex differences. Objective: Our main objective was to evaluate the dietary quality of schoolchildren and investigate whether poor HEI scores are associated with CVD risk and if there are sex differences. Methods: This was a cross-sectional study of Kuwaiti fifth graders (n = 313; 53% girls; mean age = 10.4 ± 0.4 years) who completed an adapted Block Kids 2004 food frequency questionnaire. Anthropometric, blood pressure, and biochemical data were also measured. HEI-2010 and HEI-2015 scores were calculated. Statistics: A general linear model and logistic regression were applied, controlling for moderate–vigorous physical activity (MVPA) and screen time (ST). Results: The total HEI-2010 and HEI-2015 scores were 58 and 52 points, respectively; a trend analysis indicated that more girls than boys had poor (≤50 points) HEI-2015 scores (p < 0.063). The maximum scores for total vegetables (p < 0.001), dairy (p < 0.034), and fatty acids (p < 0.01) were significantly higher in girls, while the maximum scores for whole grains (p < 0.046) and protein (p < 0.006), but not sodium (p < 0.009), were higher in boys. Obesity was inversely associated with poor total HEI 2010 and HEI 2015 scores (OR: 0.347, 95% CI: 0.234 - 0.516, p < 0.001 and OR: 0.561, 95% CI: 0.391–0.805, p < 0.002, respectively). However, the correlation was lost after adjustment for possible confounding factors. Conclusions: Dietary quality for children overall in this study was low, and there was only a weak association between poor scores and elevated blood pressure and none between scores and obesity. These findings have public health implications and warrant further investigation and attention.
Treatment of Cachexia in Gastric Cancer: Exploring the Use of Anti-Inflammatory Natural Products and Their Derivatives
Authors: Loyala Jerocin Vishani , Down Billy , Wong Enoch , Tan Benjamin
Published: April 2024

(1) Background: Gastric cancer is a significant cause of cancer-related mortality worldwide. Weight loss and malnutrition associated with cancer are linked with increased mortality rates and reduced quality of life. Cancer cachexia, characterised by the loss of skeletal muscle, is associated with approximately 20% of cancer-related deaths and differs from malnutrition in that it cannot be fully reversed by nutritional support alone. It is now recognised that the primary pathophysiological process underlying cancer cachexia is chronic inflammation leading to increased calorie consumption. Current treatments that focus on nutritional supplementation, psychological counselling, appetite stimulation and reducing inflammation are lacking in efficacy. This review focuses on the evidence supporting the potential roles of natural anti-inflammatory products and their derivatives including fatty acids, probiotics, amino acids, curcumin, fucoidan, epigallocatechin-3-gallate, ginger, resveratrol and Boswellia serrata in the management of gastric cancer cachexia. (2) Results: While natural anti-inflammatory products show promise in a number of in vitro and in vivo studies, there are only a small number of human studies available. Where present, the evidence base is heterogeneous, with varying study methodologies and outcomes. (3) Conclusions: Natural anti-inflammatory products represent a potential adjunctive therapy for gastric cancer cachexia. Further research, particularly well-designed clinical trials, is needed to elucidate their optimal role, dosing and safety profiles in the management of gastric cancer cachexia.
Synergistic Biomedical Potential and Molecular Docking Analyses of Coumarin–Triazole Hybrids as Tyrosinase Inhibitors: Design, Synthesis, In Vitro Profiling, and In Silico Studies
Authors: Kausar Rukhsana , Zahoor Ameer Fawad , Tabassum Hina , Kamal Shagufta , Ahmad Bhat Mashooq
Published: April 2024

The tyrosinase enzyme has a vital role in the browning of vegetables and fruits and the biosynthesis of melanin. In this work, we synthesized a diverse library of coumarin–triazole hybrids, and these compounds were characterized by using suitable analytical techniques. Our research work extends beyond the synthetic effort to explore the therapeutic potential of these compounds. We put the synthesized compounds through meticulous in vitro screening against the tyrosinase enzyme, and these coumarin derivatives evinced good IC50 values in the range of 0.339 ± 0.25 µM to 14.06 ± 0.92 µM. In the library of synthesized compounds, six compounds were found to be more potent than standard ascorbic acid (IC50 = 11.5 ± 1.00), and among them, 17e and 17f, being the most active, exhibited remarkable anti-tyrosinase potential, with IC50 values of 0.339 ± 0.25 μM and 3.148 ± 0.23 μM, respectively. Furthermore, an in silico modeling study was carried out to determine the key interactions of these compounds with the tyrosinase protein (PDB ID: 2Y9X) and thus to authenticate our experimental findings. The quantitative SAR studies exhibited a good correlation between the synthesized derivatives of coumarin and their anti-tyrosinase activity. The docking studies verified the experimental results, and ligand 17e showed good interaction with the core residues of tyrosinase. This study not only expands the field of coumarin–triazole hybrid synthesis but also provides valuable insights for the development of novel tyrosinase inhibitors.
Nature-Inspired 1-Phenylpyrrolo[2,1-a]isoquinoline Scaffold for Novel Antiproliferative Agents Circumventing P-Glycoprotein-Dependent Multidrug Resistance
Authors: Nevskaya Alisa A. , Purgatorio Rosa , Borisova Tatiana N. , Varlamov Alexey V. , Anikina Lada V. , Obydennik Arina Yu. , Nevskaya Elena Yu. , Niso Mauro , Colabufo Nicola A. , Carrieri Antonio , Catto Marco , de Candia Modesto , Voskressensky Leonid G. , Altomare Cosimo D.
Published: April 2024

Previous studies have shown that some lamellarin-resembling annelated azaheterocyclic carbaldehydes and related imino adducts, sharing the 1-phenyl-5,6-dihydropyrrolo[2,1-a]isoquinoline (1-Ph-DHPIQ) scaffold, are cytotoxic in some tumor cells and may reverse multidrug resistance (MDR) mediated by P-glycoprotein (P-gp). Herein, several novel substituted 1-Ph-DHPIQ derivatives were synthesized which carry carboxylate groups (COOH, COOEt), nitrile (CN) and Mannich bases (namely, morpholinomethyl derivatives) in the C2 position, as replacements of the already reported aldehyde group. They were evaluated for antiproliferative activity in four tumor cell lines (RD, HCT116, HeLa, A549) and for the ability of selectively inhibiting P-gp-mediated MDR. Lipophilicity descriptors and molecular docking calculations helped us in rationalizing the structure–activity relationships in the P-gp inhibition potency of the investigated 1-Ph-DHPIQs. As a main outcome, a morpholinomethyl Mannich base (8c) was disclosed which proved to be cytotoxic to all the tested tumor cell lines in the low micromolar range (IC50 < 20 μM) and to inhibit in vitro the efflux pumps P-gp and MRP1 responsible for MDR, with IC50s of 0.45 and 12.1 μM, respectively.
Research on the Mechanical Properties and Structural Optimization of Pipe String Joint under Deep Well Fracturing Operation
Authors: Ma Chentao , Duan Yonggang , Huang Kun , Mo Qianwen , Chen Qi , Fu Tiesong
Published: April 2024

In order to reduce the failure accidents caused by the insufficient strength of fracturing string joints, theoretical calculation and string design methods were adopted to conduct finite element calculations on commonly used long circular threads. The distribution laws of stress and contact pressure of long round threads were obtained, a non-standard special thread was designed, and a finite element model of the joint of the casing was established. Considering different make-up torques, tensile loads, and tensile torque loads within a certain range, the stress variation law of the special casing threaded joint under this design size was analyzed. Finally, the stress and contact pressure variation law on the threaded tooth was analyzed under different structures, working conditions, and wall thickness parameters. The thread strength and sealing function were compared under various parameters. The results showed that the smaller the wall thickness of the joints, the greater the contact pressure at the threaded tooth. Among them, the contact pressure of the external threaded tooth is too high, and is prone to the sticking phenomenon. The distribution of contact pressure in the middle section is relatively reasonable. Compared with the original structure, the new structure significantly reduces the contact pressure at the head and tail ends of the threaded connection, reducing the risk of sticking.