bluuebunny/crossref_metadata_2025 huggingface dataset:
Life-Related Hazards of Materials Applied to Mg–S Batteries
Authors: Siczek Krzysztof
Published: February 2022

Nowadays, rechargeable batteries utilizing an S cathode together with an Mg anode are under substantial interest and development. The review is made from the point of view of materials engaged during the development of the Mg–S batteries, their sulfur cathodes, magnesium anodes, electrolyte systems, current collectors, and separators. Simultaneously, various hazards related to the use of such materials are discussed. It was found that the most numerous groups of hazards are posed by the material groups of cathodes and electrolytes. Such hazards vary widely in type and degree of danger and are related to human bodies, aquatic life, flammability of materials, or the release of flammable or toxic gases by the latter.
Development of Renewable Energy Sources in the European Union in the Context of Sustainable Development Policy
Authors: Bórawski Piotr , Wyszomierski Rafał , Bełdycka-Bórawska Aneta , Mickiewicz Bartosz , Kalinowska Beata , Dunn James W. , Rokicki Tomasz
Published: February 2022

Renewable energy sources play a key role in decarbonizing the economy of the European Union (EU) and the world. The aim of this research is to present the development of the renewable energy sources (RES) sector in the European Union (EU), with particular emphasis on sustainable development. The EU guidelines, requirements, and directives were analyzed in order to meet the provisions of the energy policy to ensure energy and climate security. The potential of the RES was studied in the EU countries, and the possibility of its use in cogeneration with the use of local renewable resources. The results are presented in tabular, graphic, and descriptive forms. The results are presented based on the extensive literature on the subject and data from Eurostat. The data covered 2004–2019. We used different methods to evaluate the changes in the RES in the EU countries. First, we compiled descriptive statistics; second, we used the Augmented Dickey–Fuller test (ADF test); and, finally, we used the Generalized Autoregressive Conditional Heteroscedasticity model (GARCH model). Our analysis found that the EU increased the share of RES. The biggest share of energy from renewable energy sources was found in 2019 in Iceland (78%), Norway (74%), and Sweden (56%). The biggest increase in the share of RES in 2004–19 was found in Malta (8322%), Great Britain (1126%), and Luxemburg (784%). The results demonstrate the development of RES in the EU countries. However, not all countries achieved the planned goal in 2019.
Comprehensive Analysis for Electromagnetic Shielding Method Based on Mesh Aluminium Plate for Electric Vehicle Wireless Charging Systems
Authors: Wang Ke , Zuo Zhiping , Sang Lin , Zhu Xiaoqiang
Published: February 2022

As an epoch-making technique, wireless power transfer (WPT) technology has been used in electric vehicle charging devices in recent years, but the electromagnetic leakage problem has always plagued numerous researchers. The traditional wireless charging systems use a solid metal aluminium plate to shield electromagnetic leakage generally. Although it has a good shielding performance, it will seriously reduce the transmission efficiency of wireless charging systems. In this paper, an aluminium plate with a series of mesh holes of different sizes is proposed to weaken the eddy current in partial areas on the plane. Therefore, without changing the maximum magnetic induction intensity of the shielded magnetic field, the influence of the aluminium plate on the electrical parameters of the wireless power transfer system is minimized, and the transmission efficiency of the system is improved. The Ansys Maxwell software has been adopted to simulate the transfer and shielding performance. Finally, the experimental results have verified that the optimized mesh aluminium plate can reduce the interference to the transmission performance of electric vehicle wireless charging system and further improve the electromagnetic environment of the system effectively at the same time.
Workforce Planning Framework for a Mobile Call Center Considering a Special Event
Authors: Chanpanit Thanyawan , Udomsakdigool Apinanthana
Published: February 2022

Workforce planning is essential in today’s business management. If an organization can find and keep enough staff who have the right values, then they can provide high-quality service. This paper presents a workforce planning framework for selecting the best forecasting model in order to provide minimum wage and computer electricity costs for a mobile call center during the Songkran festival event, and to optimize workforce planning. The framework is constructed with four main steps: a study of a separate period; the separation of models with different data types; the simulation of models under different service levels to determine the number of customers waiting in a call center; and the evaluation of the models. The results from the proposed framework presented the best forecasting method and the optimal workforce plan. It is clear that this approach can assist in systematically selecting the best forecasting model. In addition, a workforce planner can use this framework to support workforce planning and cost evaluation in other event periods.
Implementation and Test of an IEC 61850-Based Automation Framework for the Automated Data Model Integration of DES (ADMID) into DSO SCADA
Authors: Chen Shuo , Ebe Falko , Morris Jeromie , Lorenz Heiko , Kondzialka Christoph , Heilscher Gerd
Published: February 2022

As a result of the energy transition, an increasing number of Decentralized Energy Systems (DES) will be installed in the distribution grid in the future. Accordingly, new methods to systematically integrate the growing DES in distribution power systems must be developed utilizing the constantly evolving Information and Communication Technologies (ICT). This paper proposes the Automated Data Model Integration of DES (ADMID) approach for the integration of DES into the ICT environment of the Distribution System Operator (DSO). The proposed ADMID utilizes the data model structure defined by the standard-series IEC 61850 and has been implemented as a Python package. The presented two Use Cases focus on the Supervisory Control and Data Acquisition (SCADA) on the DSO operational level following a four-stage test procedure, while this approach has enormous potential for advanced DSO applications. The test results obtained during simulation or real-time communication to field devices indicate that the utilization of IEC 61850-compliant data models is eligible for the proposed automation approach, and the implemented framework can be a considerable solution for the system integration in future distribution grids with a high share of DES. As a proof-of-concept study, the proposed ADMID approach requires additional development with a focus on the harmonization with the Common Information Model (CIM), which could significantly improve its functional interoperability and help it reach a higher Technology Readiness Level (TRL).
Electric Field Effects on Photoelectrochemical Water Splitting: Perspectives and Outlook
Authors: Boyd Stephanie J. , Long Run , English Niall J.
Published: February 2022

The grand challenges in renewable energy lie in our ability to comprehend efficient energy conversion systems, together with dealing with the problem of intermittency via scalable energy storage systems. Relatively little progress has been made on this at grid scale and two overriding challenges still need to be addressed: (i) limiting damage to the environment and (ii) the question of environmentally friendly energy conversion. The present review focuses on a novel route for producing hydrogen, the ultimate clean fuel, from the Sun, and renewable energy source. Hydrogen can be produced by light-driven photoelectrochemical (PEC) water splitting, but it is very inefficient; rather, we focus here on how electric fields can be applied to metal oxide/water systems in tailoring the interplay with their intrinsic electric fields, and in how this can alter and boost PEC activity, drawing both on experiment and non-equilibrium molecular simulation.
Statistical Assessment of Electric Shock Hazard in MV Electrical Power Substations Supplied from Networks with Non-Effectively Earthed Neutral Point
Authors: Tarko Rafał , Kmak Jarosław , Nowak Wiesław , Szpyra Waldemar
Published: February 2022

This paper focuses on the evaluation of the electric shock hazard accompanying earth faults in a non-effectively earthed medium-voltage (MV) electrical power network. This hazard depends on the duration and value of the fault current. While the fault current depends on several factors, the most important is the neutral point earthing method. The value of the fault current affects the earthing-electrode voltage value, being the basis for the assessment of electric shock hazard in MV/LV substations. The earthing-electrode voltage is also influenced by the resistance of the substation earthing, which in practice is random. Therefore, an original statistical evaluation method for assessing the electric shock hazard has been developed and presented in this paper. It is based on a statistical model of the MV/LV substation earthing resistance, worked out on the basis of experiments and measurements in real electrical power networks. This method can be used for the determination of statistical distributions of earthing-conductor voltages in real electrical power networks, and on this basis, the MV/LV substations with rates of electric shock hazard that are too high can be indicated. This also makes it possible to determine the longest permissible fault current interruption times or the highest permissible earthing resistances for specific substations. This method is also applicable when selecting the neutral point earthing method. The developed method was used in all of the above proposed areas by performing calculations on a model of a real 15 kV network with the neutral point earthed by a resistor. This analysis proves the practicability of the method both at the stage of designing power networks, and when a change in the neutral point earthing method in existing networks is being considered. Particularly valuable is also the statistical model of an earthing electrode resistance, developed on the basis of measurements in 2408 MV/LV substations, which may also be applicable in the future studies.
Extended Application and Experimental Verification of a New Erosive Burning Model Coupled Heat Transfer between Gas and Grain Based on a Star-Grain Solid Rocket Motor
Authors: Sun Lin , Ma Yanjie , Bao Futing , Liu Yang , Hui Weihua
Published: February 2022

The estimation of erosive burning is of great importance for the internal ballistics computation of a solid rocket motor (SRM) with a large aspect ratio. Because of the variety of parameters affecting erosive burning, most of the erosive burning models developed in earlier years usually contain unknown constants that need to be identified by a trial-and-error procedure for each SRM. Based on an SRM with a cylindrical grain, a new erosive burning model, which coupled the heat transfer between the gas and grain, was proven to be effective previously. To expand the scope of application of this model, in this paper, earlier and new erosive burning models were used in the transient one-dimensional internal ballistics computation, to obtain the internal ballistics for a star-grain SRM. A comparison between the computational and experimental results indicated that both the earlier and new erosive burning models can obtain results with good accuracy for a star-grain SRM. The paper shows that with no constants to be identified, the Ma model is easy to use and has the potential to predict the erosive burning rate before a firing test.
Special Issue “Advanced DC-DC Power Converters and Switching Converters”
Authors: Musumeci Salvatore
Published: February 2022

Nowadays, power electronics is an enabling technology in the energy conversion development scenario [...]
Long-Term Forest Conversion Affects Soil Stability and Humic Substances in Aggregate Fractions in Subtropical China
Authors: Meng Miaojing , Li Chong , Zhao Youpeng , Lin Jie , Liu Xin , Jia Zhaohui , Zhang Jinchi
Published: February 2022

Soil aggregates are the basic structural components of soil, which are important factors that can predict erosion resistance. However, few researchers have investigated the effects of forest conversion on the stability of soil aggregates, particularly in subtropical forests. In this study, soils from various depths (0 to 30 cm) were collected from four forest types (transformed from broadleaved forests (BMF) to combined coniferous broadleaved (CBMF), Chinese fir (FF), and bamboo forests (BF)) to determine the impacts of forest conversion on the physical and chemical properties of soil, water-stable soil aggregates, and aggregate-associated humic substances. The results showed that forest conversion had no effects on the soil’s physical properties, or the humic substances in bulk soil, but had significant effects on soil aggregates. In addition, the conversion of broadleaved forest to Chinese fir forest increased the soil stability, and to bamboo forest, decreased the soil stability. Finally, the soil’s physicochemical properties were closely related to aggregate-associated humic substances. In summary, specific forest management measures should be applied to strengthen the positive impacts and reduce the negative impacts associated with forest conversion.
Risk Prediction and Variable Analysis of Pine Wilt Disease by a Maximum Entropy Model
Authors: Hao Zhuoqing , Fang Guofei , Huang Wenjiang , Ye Huichun , Zhang Biyao , Li Xiaodong
Published: February 2022

Pine wilt disease (PWD) has caused a huge damage to pine forests. PWD is mainly transmitted by jumping diffusion, affected by insect vectors and human activities. Since the results of climate change, pine wood nematode (PWN—Bursaphelenchus xylophilus) has begun invading the temperate zones and higher elevation area. In this situation, predicting the distribution of PWD is an important part of the prevention and control of the epidemic situation. The research established the Maxent model to conduct a multi-angle, fine-scale prediction on the risk distribution of PWD. We adjusted two parameters, regularization multiplier (RM) and feature combination (FC), to optimize the model. Influence factors were selected and divided into natural, landscape, and human variables, according to the physical characteristics and spread rules of PWD. The middle-suitability regions and high-suitability regions are distributed in a Y-shape, and divided the study area into three parts. The high-suitability areas are concentrated in the region with high temperature, low elevation, and intensive precipitation. Among the selected variables, natural factors still play the most important role in the distribution of the disease, and human factors and landscape factors are also worked well. The permutation importance of factors is different due to differences in climate and other conditions in different regions. The multi-angle, fine-scale model can help provide useful information for effective control and tactical management of PWD.
The Impact of Resources on the Adaptability of State Forest Companies. Some Evidence from the State Forests National Forest Holding
Authors: Cyfert Szymon , Dyduch Wojciech , Zastempowski Maciej
Published: February 2022

Structural, economic, and climate changes human activity and the growing complexity of the business environment significantly affect the forestry sector, which faces the need to adapt to the dynamically changing environment by strategic development of appropriate resources and skills. In this paper, we attempted to take on the managerial lens of dynamic capabilities, i.e., the abilities to adapt to the changes in the business environment to analyze whether the forestry sector is able to strategically develop resources that influence adaptability. We have attempted to demonstrate how the resources of the forest enterprises affect the adaptability described by the dynamic capabilities construct. Bearing in mind the importance of State Forests, we collected data from 129 forest districts in Poland and applied the ordered logistic regression to identify models that show the impact of specific categories of resources onto the forestry sector adaptability, described by the construct of dynamic capabilities. The results suggest that the forest districts strategically investing in technological, as well as human resources and skills, have higher chances of maintaining and developing the ability to adapt to the constantly changing economic environment. At the same time, our findings show that financial, reputational, and marketable resources and skills have no impact on the adaptability of the State Forests National Forest Holding.
COVID-19 Anxiety as a Moderator of the Relationship between Organizational Change and Perception of Organizational Politics in Forestry Public Sector
Authors: Larasatie Pipiet , Fitriastuti Triana , Yovi Efi Yuliati , Purnomo Herry , Nurrochmat Dodik Ridho
Published: February 2022

In addition to an outstanding commitment to the Sustainable Development Goals’ (SDG) agenda to good governance (goal no. 16), there is an argument that the SDGs can only be achieved through good governance with strong political institutions and processes. In Indonesia, a new era in politics has been marked with the new leadership of Joko Widodo (the current Indonesian President) who has a vision to reform the Indonesian bureaucracy. One of the bureaucratic reform implementations is the merging of the Ministry of Forestry and the Ministry of Environment into the Ministry of Environment and Forestry (MoE). In this kind of organizational change, employees may have increased perceptions of organizational politics and feelings of uncertainty and anxiety. This effect is suspected to be exacerbated by the impact of the COVID-19 pandemic. This article, therefore, aims to investigate the effects of organizational change in the public sector. Based on a survey of 112 state civil apparatuses in the forestry sector in Indonesia, we found that organizational change is positively related to employees’ perception of organizational politics. Nevertheless, our most intriguing finding is that the COVID pandemic situation has decreased employees’ perception of organizational politics. This is because political behaviors are difficult to perform in virtual working settings due to reduced face-to-face interaction and limited non-verbal cues.
Verifying the Lifting and Slewing Dynamics of a Harvester Crane with Possible Levelling When Operating on Sloping Grounds
Authors: Mergl Václav , Kašpárek Jaroslav
Published: February 2022

This paper focuses on the force and torque load of a harvester hydraulic crane employed on sloping grounds, both levelled and not levelled. Field research was conducted for this purpose and the results were compared with a dynamic analysis of the crane in MSC Adams. It was found that levelling the slewing platform of the crane is necessary for use on sloping grounds, primarily because the effect on the force and lifting torques is reduced. The research showed that when the slope of the slewing gear is up to −12°, the lifting torque reaches a higher maximum lifting force than when the slewing gear is in a horizontal position (0°). As part of the theoretical verification by a dynamic analysis of the crane and the AH6 machine, a different pressure was detected in the lifting cylinder of the crane compared to the field research. The total deviation between the simulation and the field research was 9.82%. The slewing torque of the hydraulic crane without the slewing bearing being levelled can be characterized 97.38% by a parabola whose vertex is located in front of the front part of the machine and falls as the crane moves left or right. Overall, it can be determined that when the crane rotates up a slope, whether it is from left or right, the slewing torque reaches the lowest values, and its value increases as the crane gets closer to the front of the machine (along the longitudinal axis of the machine). This change in the slewing torque is then characterized by a parabola. Furthermore, an effect was observed of the slewing gear slope on the lifting torque, which reached higher values in a tilted position than on a flat surface.
Yeast Hybrids in Brewing
Authors: Winans Matthew J.
Published: February 2022

Microbiology has long been a keystone in fermentation, and innovative yeast molecular biotechnology continues to represent a fruitful frontier in brewing science. Consequently, modern understanding of brewer’s yeast has undergone significant refinement over the last few decades. This publication presents a condensed summation of Saccharomyces species dynamics with an emphasis on the relationship between; traditional Saccharomyces cerevisiae ale yeast, S. pastorianus interspecific hybrids used in lager production, and novel hybrid yeast progress. Moreover, introgression from other Saccharomyces species is briefly addressed. The unique history of Saccharomyces cerevisiae and Saccharomyces hybrids is exemplified by recent genomic sequencing studies aimed at categorizing brewing strains through phylogeny and redefining Saccharomyces species boundaries. Phylogenetic investigations highlight the genomic diversity of Saccharomyces cerevisiae ale strains long known to brewers for their fermentation characteristics and phenotypes. The discovery of genomic contributions from interspecific Saccharomyces species into the genome of S. cerevisiae strains is ever more apparent with increasing research investigating the hybrid nature of modern industrial and historical fermentation yeast.
Modelling Fish Growth with Imperfect Data: The Case of Trachurus picturatus
Authors: Neves Ana , Vieira Ana Rita , Sequeira Vera , Silva Elisabete , Silva Frederica , Duarte Ana Marta , Mendes Susana , Ganhão Rui , Assis Carlos , Rebelo Rui , Magalhães Maria Filomena , Gil Maria Manuel , Gordo Leonel Serrano
Published: February 2022

Growth modelling is essential to inform fisheries management but is often hampered by sampling biases and imperfect data. Additional methods such as interpolating data through back-calculation may be used to account for sampling bias but are often complex and time-consuming. Here, we present an approach to improve plausibility in growth estimates when small individuals are under-sampled, based on Bayesian fitting growth models using Markov Chain Monte Carlo (MCMC) with informative priors on growth parameters. Focusing on the blue jack mackerel, Trachurus picturatus, which is an important commercial fish in the southern northeast Atlantic, this Bayesian approach was evaluated in relation to standard growth model fitting methods, using both direct readings and back-calculation data. Matched growth parameter estimates were obtained with the von Bertalanffy growth function applied to back-calculated length at age and the Bayesian fitting, using MCMC to direct age readings, with both outperforming all other methods assessed. These results indicate that Bayesian inference may be a powerful addition in growth modelling using imperfect data and should be considered further in age and growth studies, provided relevant biological information can be gathered and included in the analyses.
Note on the Bulk Estimate of the Energy Dissipation Rate in the Oceanic Bottom Boundary Layer
Authors: Ruan Xiaozhou
Published: February 2022

The dissipation of the kinetic energy (KE) associated with oceanic flows is believed to occur primarily in the oceanic bottom boundary layer (BBL), where bottom drag converts the KE from mean flows to heat loss through irreversible mixing at molecular scales. Due to the practical difficulties associated with direct observations on small-scale turbulence close to the seafloor, most up-to-date estimates on bottom drag rely on a simple bulk formula (CdU3) proposed by G.I. Taylor that relates the integrated BBL dissipation rate to a drag coefficient (Cd) as well as a flow magnitude outside of the BBL (U). Using output from several turbulence-resolving direct numerical simulations, it is shown that the true BBL-integrated dissipation rate is approximately 90% of that estimated using the classic bulk formula, applied here to the simplest scenario where a mean flow is present over a flat and hydrodynamically smooth bottom. It is further argued that Taylor’s formula only provides an upper bound estimate and should be applied with caution in the future quantification of BBL dissipation; the performance of the bulk formula depends on the distribution of velocity and shear stress near the bottom, which, in the real ocean, could be disrupted by bottom roughness.
Interannual Bottom-Intensified Current Thickening Observed on the Continental Slope Off the Southeastern Coast of Hokkaido, Japan
Authors: Nagano Akira , Hasegawa Takuya , Ariyoshi Keisuke , Matsumoto Hiroyuki
Published: February 2022

By rotary empirical orthogonal function and coastal-trapped wave mode analyses, we analyzed current velocity data, collected from 2001 to 2016. The data were obtained by an acoustic Doppler current profiler, deployed upward at a location of 41°39.909′ N, 144°20.695′ E, on a 2630-m deep continental slope seabed off the southeastern coast of Hokkaido, Japan. The results indicate that the current intensifies toward the bottom and is directed nearly toward the shore, reaching an average speed of ~2.5 cm s−1 just above the bottom. The thickness of the along-slope northward component of the bottom-intensified current varied within the range of 50–350 m. We found that the current thickness change was caused by oceanic barotropic disturbances, produced by the intensification of the Aleutian Low, largely related to the El Niño–Southern Oscillation and modified through the excitation of bottom-trapped modes of coastal-trapped waves. This finding improves the prediction accuracy of the the bottom-intensified current change, being beneficial for suspended sediment studies, construction and maintenance of marine structures, planning of deep drilling, and so on.
On the Computational Modeling of Inclined Brine Discharges
Authors: Papakonstantis Ilias G. , Papanicolaou Panos N.
Published: February 2022

In this paper, five computational approaches are used to model bulk flow parameters of inclined round negatively buoyant jets. More specifically, an integral model employing Gaussian distributions for velocity and apparent acceleration of gravity, proposed in earlier study, is implemented with two different entrainment formulae. The remaining three computational approaches include an integral model known as EMA, which takes into consideration the fluid detachment occurring in the inner side of the flow near the terminal height, the widely known commercial model Corjet and analytical solutions that were proposed in a previous study. Predictions are provided for the maximum centerline height and its horizontal position, the terminal height of the upper jet boundary, the horizontal distance to the points where the jet centerline and the upper jet boundary return to the source level, the centerline dilution at the maximum height and the centerline dilution at the return point. Detailed comparisons are made in dimensionless form between the estimations provided by the models and a wide range of experimental data for discharge angles between 15° and 90°. Conclusions are drawn regarding the performance of the five computational approaches.
Evaluation of the Physicochemical and Functional Properties of Aquasoya (Glycine max Merr.) Powder for Vegan Muffin Preparation
Authors: Kim Yoon-Ha , Shin Weon-Sun
Published: February 2022

Recent concerns on health and sustainability have prompted the use of legumes as a source of plant-based proteins, resulting in the application of their cooking water as a substitute for egg whites. In this study, the cooking water of yellow soybeans was powdered, and, subsequently, the nutritional and functional characteristics of powders from yellow soybeans (YSP), chickpeas (CHP), and egg whites (EWP) were compared. The main components of these powders (total polyphenol, total carbohydrate, and protein), along with their hydration properties (hygroscopicity, water solubility index, and water/oil holding capacities), and emulsifying and foaming properties, were identified. The muffins prepared with YSP, CHP, and EWP were analyzed to determine their basic characteristics, such as volume, baking loss, and sensory attributes. The results of the powder analyses indicated that YSP was significantly superior to CHP and EWP, particularly in terms of holding capacities, and emulsion and foam stabilities. The sensory evaluation results showed that there was no statistically significant difference in overall acceptance among the muffin samples. Therefore, YSP can be used as an alternative to CHP or EWP, and applied as a novel ingredient in various vegan products.
Effects of Gum Arabic Coatings Enriched with Lemongrass Essential Oil and Pomegranate Peel Extract on Quality Maintenance of Pomegranate Whole Fruit and Arils
Authors: Kawhena Tatenda Gift , Opara Umezuruike Linus , Fawole Olaniyi Amos
Published: February 2022

The effects of gum arabic coatings combined with lemongrass oil and/or pomegranate peel extract on freshly harvested mature ‘Wonderful’ pomegranate fruit were studied. Fruit were coated with gum arabic (GA) (1.5% w/v) alone or enriched with lemongrass oil (LM) (0.1% v/v) and/or pomegranate peel extract (PP) (1% w/v). Fruit were packed into standard open top ventilated cartons (dimensions: 0.40 m long, 0.30 m wide and 0.12 m high), and stored for 6 weeks at 5 ± 1 °C (90% RH). Evaluations were made every 2 weeks of cold storage and after 5 d of shelf life (20 °C and 65% RH). Fruit coated with GA + PP (4.09%) and GA + PP + LM (4.21%) coatings recorded the least cumulative weight loss compared to the uncoated control (9.87%). After 6 weeks, uncoated control and GA + PP + LM recorded the highest (24.55 mg CO2Kg−1h−1) and lowest (10.76 mg CO2Kg−1h−1) respiration rate, respectively. Coating treatments reduced the incidence of decay and treatments GA + LM + PP and GA + PP recorded the highest total flavonoid content between 2 and 6 weeks of storage. The findings suggest that GA coatings with/without LM and PP can be a beneficial postharvest treatment for ‘Wonderful’ pomegranates to reduce weight loss and decay development during cold storage.
A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of Salmonella Typhimurium
Authors: Fu Shiqian , Yang Xinyan , Pang Lidong , Cheng Shasha , Song Danliangmin , Qin Xue , Man Chaoxin , Jiang Yujun
Published: February 2022

Salmonella Typhimurium (S. Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of S. Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect S. Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to S. Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect S. Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to S. Typhimurium, ranging from 3.7 × 102 to 3.7 × 105 cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of S. Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 102 cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify S. Typhimurium in foodstuffs.
The Effect of Dietary Supplementation with Resveratrol on Growth Performance, Carcass and Meat Quality, Blood Lipid Levels and Ruminal Microbiota in Fattening Goats
Authors: Shen Yujian , Jiang Yuhang , Zhang Sanbao , Zou Juhong , Gao Xiaotong , Song Ying , Zhang Yu , Hu Yan , Huang Yanna , Jiang Qinyang
Published: February 2022

This study investigated the effects of resveratrol (RES) supplementation on the growth performance, carcass and meat quality, blood lipid levels and ruminal bacterial microbiota of fattening goats. A total of forty castrated Nubian goats (28.25 ± 0.26 kg body weight) were randomly divided into four groups and provided with diets containing different levels of RES (0, 150, 300 and 600 mg/kg) for 120 d. The results showed that RES increased redness and intramuscular fat content, whilst reducing shear force in the longissimus dorsi muscle of goats (p < 0.05). In addition, the final weight, average daily gain, hot carcass weight, net meat weight, carcass lean percentage and eye muscle area of goats were significantly increased in the 150 mg/kg RES group compared with the other three groups, while those in the 600 mg/kg RES group significantly decreased (p < 0.05). RES significantly decreased serum triacylglycerol and LDL-C contents (p < 0.05), and increased HDL-C content and the HDL-C/TC ratio (p < 0.05). Supplementation with 150 mg/kg RES also increased the proportion of Acetitomaculum and Moryella, genera comprising short-chain fatty acid-producing bacteria. The present study indicated that an appropriate supplemental level of RES could improve the growth performance, neat percentage, meat quality, ruminal microbiota and serum lipid levels of fattening goats.
Tiger Nut (Cyperus esculentus L.): Nutrition, Processing, Function and Applications
Authors: Yu Yali , Lu Xiaoyu , Zhang Tiehua , Zhao Changhui , Guan Shiyao , Pu Yiling , Gao Feng
Published: February 2022

The tiger nut is the tuber of Cyperus esculentus L., which is a high-quality wholesome crop that contains lipids, protein, starch, fiber, vitamins, minerals and bioactive factors. This article systematically reviewed the nutritional composition of tiger nuts; the processing methods for extracting oil, starch and other edible components; the physiochemical and functional characteristics; as well as their applications in food industry. Different extraction methods can affect functional and nutritional properties to a certain extent. At present, mechanical compression, alkaline methods and alkali extraction–acid precipitation are the most suitable methods for the production of its oil, starch and protein in the food industry, respectively. Based on traditional extraction methods, combination of innovative techniques aimed at yield and physiochemical characteristics is essential for the comprehensive utilization of nutrients. In addition, tiger nut has the radical scavenging ability, in vitro inhibition of lipid peroxidation, anti-inflammatory and anti-apoptotic effects and displays medical properties. It has been made to milk, snacks, beverages and gluten-free bread. Despite their ancient use for food and feed and the many years of intense research, tiger nuts and their components still deserve further exploitation on the functional properties, modifications and intensive processing to make them suitable for industrial production.
Postmortem Interval Estimation: New Approaches by the Analysis of Human Tissues and Microbial Communities’ Changes
Authors: Zapico Sara C. , Adserias-Garriga Joe
Published: February 2022

There are several methodologies available to estimate time since death based on different changes that a corpse undergoes after death. However, these methods are imprecise due to the decomposition process being affected by several factors, principally temperature and humidity. Current trends for the determination of the Postmortem Interval (PMI) attempt to estimate the PMI in a quantifiable manner, based on chemical changes on and in the body, summarized in the field of “thanatochemistry”. Although these methodologies have improved PMI estimates, additional research has been developed to increase the accuracy and precision of this determination. As a result, the fields of “thanatobiology” and “thanatomicrobiome” have emerged. Thanatobiology is based on the estimation of the PMI from DNA/RNA degradation, signaling pathways of cell death, and protein analysis. Thanatomicrobiome refers to changes in the bacterial communities as a consequence of the decomposition process. Although both approaches seem to improve PMI estimates, applications of thanatobiology methodologies are more appropriate in the first phases of decomposition, while thanatomicrobiome analyses are applicable in advanced stages. Further research is needed in these new fields in order to establish their applicability in forensic cases. This is a review of the current state-of-the-art methodology in these two subfields.
An Extreme Case of Liver Adenomatosis: Are They All the Same?
Authors: Chálim Rebelo Carolina , Moura Diogo Bernardo , Flor de Lima Margarida , Dutra Eduardo , Amaral Rui , Pereira José Renato , Duarte Maria Antónia
Published: February 2022

Hepatocellular adenoma (HCA) is a rare and benign liver tumor that affects predominantly young and middle-aged women, especially between 30–40 years old. Liver adenomatosis (LA) is defined as the presence of 10 or more HCA. There are authors that report eight different subtypes of HCA, that correlates with clinical and histopathological features, being the inflammatory subtype the most common. We present a case of a 32-year-old Caucasian woman with a history of self-limited episodes of right abdominal pain and an abdominal ultrasound with multiple hypoechogenic liver nodules, suspected of metastasis. She was taking combined oral contraceptive for 6 years. Magnetic Resonance Imaging (MRI) disclosed around 40 nodules, suggestive of HCA. Liver biopsy confirmed HCA, inflammatory subtype. Oral contraceptive was stopped and control MRI 6 months later disclosed reduction of nodules’ dimensions. Management of patients with LA should be based on the size of the largest tumor, as clinical presentation and risk of bleeding or malignancy do not differ between patients with single or multiple HCAs. However, even with biopsy, there is a risk of missampling, raising concern about the real risk of bleeding and malignant potential in patients with different subtypes coexisting in the same liver.
Upper Gastrointestinal Tract Associated Lesions in Patients with Newly Diagnosed Celiac Disease
Authors: Enache Iulia , Balaban Daniel Vasile , Vasilescu Florina , Jurcut Ciprian , Ionita-Radu Florentina , Popp Alina , Matei Dumitru , Jinga Mariana
Published: February 2022

(1) Background: Currently available guidelines require upper gastrointestinal (GI) endoscopy with biopsy sampling for adult celiac disease (CD) diagnosis. Based on the pediatric experience, there has been a growing interest if serology-based diagnosis would be possible for adult CD also. Our aim was to analyze the associated upper GI tract lesions in newly diagnosed CD patients, to see if significant associated pathology is detected during index endoscopy, which might impact patient management not related to CD. (2) Methods: We performed a retrospective analysis of newly diagnosed CD cases diagnosed over a period of 7 years (2014–2020). Demographic, clinical, laboratory, endoscopy and histopathology data were collected from the patients’ charts. Diagnosis was set according to ACG Guideline 2013. (3) Results: Altogether 79 patients were recruited for this study purpose, 75.9% female, median age 39 years. All patients had positive CD-specific serology and atrophic mucosal injury in duodenal biopsy samples. Besides villous atrophy, associated endoscopic findings were detected in 42/79 (53.16%) of patients. Most of the gastric lesions were minor endoscopic findings—small sliding hiatal hernias, non-specific chronic gastritis, but we also found two cases of peptic ulcers, one case of metaplastic gastritis, six cases of atrophic gastritis and one subepithelial lesion. Only one patient had changes in the duodenum except CD-related findings—an inflammatory polyp in the duodenal bulb. No malignancies were found. (4) Conclusions: In our cohort, there was a significant number of newly diagnosed CD patients who had associated lesions during the index upper GI endoscopy, but most of them were minor endoscopic findings.
Formulation and Evaluation of Topical Nano-Lipid-Based Delivery of Butenafine: In Vitro Characterization and Antifungal Activity
Authors: Zafar Ameeduzzafar , Imam Syed Sarim , Alruwaili Nabil K. , Yasir Mohd , Alsaidan Omar Awad , Alshehri Sultan , Ghoneim Mohammed M. , Khalid Mohammad , Alquraini Ali , Alharthi Salman S.
Published: February 2022

The present research work was designed to prepare butenafine (BN)-loaded bilosomes (BSs) by the thin-film hydration method. BN is a sparingly water-soluble drug having low permeability and bioavailability. BSs are lipid-based nanovesicles used to entrap water-insoluble drugs for enhanced permeation across the skin. BSs were prepared by the thin-film hydration method and optimized by the Box–Behnken design (BBD) using lipid (A), span 60 (B), and sodium deoxycholate (C) as independent variables. The selected formulation (BN-BSo) was converted into the gel using Carbopol 940 as a gelling agent. The prepared optimized gel (BN-BS-og) was further evaluated for the gel characterization, drug release, drug permeation, irritation, and anti-fungal study. The optimized bilosomes (BN-BSo) showed a mean vesicle size of 215 ± 6.5 nm and an entrapment efficiency of 89.2 ± 1.5%. The DSC study showed that BN was completely encapsulated in the BS lipid matrix. BN-BSog showed good viscosity, consistency, spreadability, and pH. A significantly (p < 0.05) high release (81.09 ± 4.01%) was achieved from BN-BSo compared to BN-BSog (65.85 ± 4.87%) and pure BN (17.54 ± 1.37 %). The permeation study results revealed that BN-BSo, BN-BSog, and pure BN exhibited 56.2 ± 2.7%, 39.2 ± 2.9%, and 16.6 ± 2.3%. The enhancement ratio of permeation flux was found to be 1.4-fold and 3.4-fold for the BN-BS-og and pure BN dispersion. The HET-CAM study showed that BN-BSog was found to be nonirritant as the score was found within the limit. The antifungal study revealed a significant (p < 0.05) enhanced antifungal activity against C. albicans and A. niger. The findings of the study revealed that BS is an important drug delivery system for transdermal delivery.
The Representation of the Holocaust in Israeli Society and Its Implications on Conceptions of Democracy and Human Rights of “Others”
Authors: Keynan Irit , Wolff Noga
Published: February 2022

Much has been written about the representation of the Holocaust in Israel, but there is less awareness to its effects on attitudes toward democracy and the universal meaning of human rights. Representations of the Holocaust by Israeli socialization agents usually focus on hatred toward Jews, disregarding the broader theoretical-ideological context. This tendency is typical to groups that suffered such severe traumas in their past. Nonetheless, we argue that it does not allow a healing process and fosters a reduced perspective on the essential principles of democracy. It also particularizes the concept of human rights, thus excluding those of “others,” such as Palestinians. We further argue that a more extensive perspective on the Holocaust, which includes an understanding of Nazism within an ideological mosaic that denies democratic principles and humanity, may strengthen Israelis’ identification with democratic principles and universal human rights. We analyze the different approaches to teaching the Holocaust in the context of the collective trauma and explore their impact on society’s sense of victimhood and moral injury. The paper ends with a suggestion for further research that will explore the possibility that a school curriculum that emphasizes universal lessons will enable the memorialization of the Holocaust without succumbing to nationalistic perceptions.
The Complete Chloroplast Genome of Carya cathayensis and Phylogenetic Analysis
Authors: Shen Jianshuang , Li Xueqin , Chen Xia , Huang Xiaoling , Jin Songheng
Published: February 2022

Carya cathayensis, an important economic nut tree, is narrowly endemic to eastern China in the wild. The complete cp genome of C. cathayensis was sequenced with NGS using an Illumina HiSeq2500, analyzed, and compared to its closely related species. The cp genome is 160,825 bp in length with an overall GC content of 36.13%, presenting a quadripartite structure comprising a large single copy (LSC; 90,115 bp), a small single copy (SSC; 18,760 bp), and a pair of inverted repeats (IRs; 25,975 bp). The genome contains 129 genes, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. A total of 252 simple sequence repeats (SSRs) and 55 long repeats were identified. Gene selective pressure analysis showed that seven genes (rps15, rpoA, rpoB, petD, ccsA, atpI, and ycf1-2) were possibly under positive selection compared with the other Juglandaceae species. Phylogenetic relationships of 46 species inferred that Juglandaceae is monophyletic, and that C. cathayensis is sister to Carya kweichowensis and Carya illinoinensis. The genome comparison revealed that there is a wide variability of the junction sites, and there is higher divergence in the noncoding regions than in coding regions. These results suggest a great potential in phylogenetic research. The newly characterized cp genome of C. cathayensis provides valuable information for further studies of this economically important species.
scInTime: A Computational Method Leveraging Single-Cell Trajectory and Gene Regulatory Networks to Identify Master Regulators of Cellular Differentiation
Authors: Xu Qian , Li Guanxun , Osorio Daniel , Zhong Yan , Yang Yongjian , Lin Yu-Te , Zhang Xiuren , Cai James J.
Published: February 2022

Trajectory inference (TI) or pseudotime analysis has dramatically extended the analytical framework of single-cell RNA-seq data, allowing regulatory genes contributing to cell differentiation and those involved in various dynamic cellular processes to be identified. However, most TI analysis procedures deal with individual genes independently while overlooking the regulatory relations between genes. Integrating information from gene regulatory networks (GRNs) at different pseudotime points may lead to more interpretable TI results. To this end, we introduce scInTime—an unsupervised machine learning framework coupling inferred trajectory with single-cell GRNs (scGRNs) to identify master regulatory genes. We validated the performance of our method by analyzing multiple scRNA-seq data sets. In each of the cases, top-ranking genes predicted by scInTime supported their functional relevance with corresponding signaling pathways, in line with the results of available functional studies. Overall results demonstrated that scInTime is a powerful tool to exploit pseudotime-series scGRNs, allowing for a clear interpretation of TI results toward more significant biological insights.