bluuebunny/crossref_metadata_2025 huggingface dataset:
Explaining the Factors Affecting Customer Satisfaction at the Fintech Firm F1 Soft by Using PCA and XAI
Authors: Khanal Mohan , Khadka Sudip Raj , Subedi Harendra , Chaulagain Indra Prasad , Regmi Lok Nath , Bhandari Mohan
Published: January 2023

The most significant and rapidly expanding fintech services in Nepal are provided by several fintech firms. Customer satisfaction must be compared side by side even if every organization has made an effort to expand the usage of services. Many studies have concentrated on evaluating the impact of various factors on customer satisfaction, but significantly fewer studies have been conducted to explore the factors and focus of machine learning. Based on the planned behavioural theory (TPB), the study is concentrated on exploring and evaluating customer satisfaction on a different stimulus offered by F1 Soft (a fintech firm in nepal), customers’ loyalty and the compatibility they gain through the company’s services. By exploring various factors affecting customer satisfaction by using principal component analysis (PCA) and explainable AI (XAI), the study explored the eight factors (customer service, compatibility, ease of use, assurance, loyalty intention, technology perception, speed and firm’s innovativeness) which affect customer satisfaction individually. Furthermore, by using support vector machine (SVM) and logistic regression (LR), the major contributing factors are explained with local interpretable model-agnostic explanation (LIME) and Shapley additive explanations (SHAP). SVM holds the training accuracy of 89.13% whereas LR achieves 87.88%, and both algorithms show that compatibilty issues consider the major contributing factor for customer satisfaction. Contributing toward different dimensions, determinants, and the results of customer satisfaction in fintech, the study suggests how fintech companies must integrate factors affecting customer satisfaction in their system for further process development.
Perceptions of Turkish Forest Firefighters on In-Service Trainings
Authors: Safak Ismail , Okan Taner , Karademir Devrim
Published: January 2023

Forest ecosystems in the Mediterranean Basin experience frequent forest fires, and the risk of hazards to people and assets is expected to increase in the face of climate change. Turkey is one of the countries in the Mediterranean Basin that has faced extreme forest fires in recent years. Forest firefighters are key actors at the forefront of fighting forest fires, and their effectiveness is largely related to in-service training. This study investigates the differences in in-service training and knowledge competencies of forest firefighters working in five fire-sensitive regions in Turkey. The results of the study are based on a survey of 975 forest firefighters. Sociodemographic data are presented as frequency, mean, and percentages. The Kruskal–Wallis H test was used to determine the differences in the perceptions of forest firefighters in the five fire-sensitive regions. It was determined that 89.74% of forest firefighters participated in in-service training, and the participation in in-service training was highest in 1st degree fire sensitive regions. Turkish forest firefighters have a strong perception that in-service training is sufficient in terms of number and content, their knowledge and experience deficiencies are eliminated, and their capacities are increased.
Molecular Identification of Photobacterium damselae in Wild Marine Fish from the Eastern Mediterranean Sea
Authors: Morick Danny , Maron Yuval , Davidovich Nadav , Zemah-Shamir Ziv , Nachum-Biala Yaarit , Itay Peleg , Wosnick Natascha , Tchernov Dan , Harrus Shimon
Published: January 2023

Infectious diseases caused by marine bacterial pathogens inflict increasing economic losses to fisheries and aquaculture, while also posing a growing risk to public health and affected species conservation. In this study, four wild marine fish species were collected at five fishing sites in Israel, divided into two regions—north (Acre, Haifa, Shefayim) and center-south (Tel-Aviv and Ashdod), and screened for Photobacterium damselae. An initial screening was carried out using PCR analysis with specifically designed primers on DNA extracted from livers and kidneys. P. damselae-positive samples had their 16S rRNA amplicons sequenced. Later, an attempt to specify relevant sub-species was performed, using a three-layered gene screen: Car, ureC and toxR. Of 334 fish samples, 47 (14%) were found to be P. damselae-positive, of which 20 were identified as P. damselae subsp. piscicida (Phdp), two as P. damselae subsp. damselae (Phdd) and 25 could not be identified to subspecies. Our results strengthen the view that fish residing in a polluted environment are receptive of pathogenic microorganisms. To assess how the presence of pathogens may affect population management and conservation, this research should be followed by studies aimed at: (i) quantifying levels of pollutants that may affect pathogen emergence, and (ii) creating a standard pollution-level index as a basis for setting criteria, above which authorities should take measures of precaution.
Valorization of Lignocellulosic Wastes Material for Efficient Adsorption of a Cationic Azo Dye and Sludge Recycling as a Reinforcement of Thermoplastic Composite
Authors: Jabri Maria , Dehmani Younes , Loulidi Ilyasse , Kali Abderahim , Amar Abdelouahed , Lgaz Hassane , Hadey Chaimaa , Boukhlifi Fatima
Published: January 2023

This work explored the adsorption of Malachite Green (MG) dye by Acorn Pericarp (AP) in the context of biomass valorization. The Acorn Pericarp was analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction. The adsorption capacity under optimal experimental conditions was studied at different adsorbent doses, the initial concentration times of the dye and pH. The results presented in this work on the adsorption kinetics of MG showed that the pseudo-first-order model (R2 = 0.9971) better described the adsorption kinetics at 10−5 M. The experimental isotherms showed that Acorn Pericarp adsorption followed the Langmuir isotherm model (R2 = 0.9889). The thermodynamic study showed that MG adsorption is endothermic (ΔH° > 0) and spontaneous (ΔG° < 0). For a sustainable industry, the sludge was converted into reinforcement of polystyrene using in-situ polymerization with 10% by weight of filler. A morphological and structural analysis was performed using SEM and FTIR, the results of characterization showed that the AP sludge was incorporated well into the PS matrix.
Glycemic Responses of Milk and Plant-Based Drinks: Food Matrix Effects
Authors: Shkembi Blerina , Huppertz Thom
Published: January 2023

The consumption of food items containing digestible carbohydrates in food products leads to postprandial increases in blood glucose levels and glycemic responses. The extent to which these occur depends on many factors, including concentration and type of carbohydrate, but also other physicochemical properties of the food matrix, which determine the rate of uptake of monosaccharides into the bloodstream, including product structure and factors affecting gastric emptying. For milk, control of postprandial glycemic responses appears to be multifaceted, including a controlled rate of gastric emptying, a rate of glucose and galactose uptake into the bloodstream controlled by enzymatic hydrolysis, as well as stimulated insulin secretion to enhance uptake of blood glucose from the bloodstream. Altogether, this allows milk to deliver comparatively high levels of carbohydrate with limited glycemic responses. For plant-based drinks positioned as milk alternatives, however, compositional differences (including carbohydrate type and concentration) as well as matrix factors limiting control over gastric emptying and insulin secretion can, in some cases, lead to much stronger glycemic responses, which are undesirable in relation to non-communicable diseases, such as type-2 diabetes. This review discusses glycemic responses to milk and plant-based drinks from this perspective, focusing on mechanistic insights and food matrix effects.
Research on the Relationship between the Amylopectin Structure and the Physicochemical Properties of Starch Extracted from Glutinous Rice
Authors: Wang Bingqing , Xu Jialu , Guo Dandan , Long Changzhi , Zhang Zhongxin , Cheng Ying , Huang Huiying , Wen Peng , He Haohua , He Xiaopeng
Published: January 2023

Glutinous rice has very low amylose content and is a good material for determining the structure and physicochemical properties of amylopectin. We selected 29 glutinous rice varieties and determined the amylopectin structure by high-performance anion exchange chromatography with the pulsed amperometric detection method. We also determined the correlation between amylopectin structure and the physicochemical properties of starch extracted from these varieties. The results showed that the amylopectin chain ratio Σdegree of polymerization (DP) ≤ 11/ΣDP ≤ 24 of 29 glutinous rice varieties was greater than 0.26, signifying that these varieties contained type II amylopectin. The results of the correlation analysis with gelatinization temperature showed that ΣDP 6–11 was significantly negatively correlated with the onset gelatinization temperature (GT) (TO), peak GT (TP), and conclusion GT (TC). Among the thermodynamic properties, ΣDP 12–24 was significantly positively correlated with To, Tp, and Tc, ΣDP 25–36 was significantly negatively correlated with To, Tp, and Tc, and ΣDP ≥ 37 had no correlation with the thermodynamic properties. The results of correlation analysis with RVA spectrum characteristic values showed that ΣDP 6–11 was significantly negatively correlated with hot paste viscosity (HPV), cool paste viscosity (CPV), consistency viscosity (CSV), peak time (PeT), and pasting temperature (PaT) among the Rapid Visco Analyzer (RVA) profile characteristics, ΣDP 12–24 was significantly positively correlated with HPV, CPV, CSV, PeT, and PaT, and ΣDP ≥ 25 had no correlation with the viscosity characteristics. Therefore, we concluded that the amylopectin structure had a greater effect on the TO, TP, TC, ΔH and peak viscosity, HPV, CPV, CSV, PeT, and PaT. The glutinous rice varieties with a higher distribution of short chains and a lower distribution of medium and long chains in the amylopectin structure resulted in lower GT and RVA spectrum characteristic values.
Evaluation and Optimization of Quality Based on the Physicochemical Characteristics and Metabolites Changes of Qingpi during Storage
Authors: Cheng Yunxia , Wu Cui , Liu Zhenying , Song Pingping , Xu Bo , Chao Zhimao
Published: January 2023

Qingpi, the dried immature pericarp of Citrus reticulata Blanco, is a commonly used medicinal food with some health-promoting benefits. In general, it is essential that Qingpi be stored for a period of time, but there are no reports about the number of storage years needed to obtain the best quality of Qingpi. Our aim was to determine the best storage time of Qingpi by studying the physicochemical properties and metabolite changes in product stored from 1 to 5 years. As a result, the color of Qingpi became darker during storage. Both the levels of three flavonoids (hesperidin, nobiletin, and tangeretin) and total flavonoids (TFs) and the antioxidant activity decreased during storage and the total phenolics (TPs) content fluctuated during storage. Cluster analysis was performed on the color parameters measured using a color difference meter, revealing that the color of Qingpi differed before and after 3 years of storage. A total of 9 special differential metabolites were identified that could be used to distinguish the storage years of Qingpi. This is the first study to report the quality changes of Qingpi during storage. The optimized results of the quality evaluation indicated that Qingpi should be stored for no more than 3 years.
Small Peptides Isolated from Enzymatic Hydrolyzate of Pneumatophorus japonicus Bone Promote Sleep by Regulating Circadian Rhythms
Authors: Wang Junbao , Zhang Lu , Tao Ningping , Wang Xichang , Deng Shanggui , Li Mingyou , Zu Yao , Xu Changhua
Published: January 2023

Due to the high addiction and side effects of medicines, people have increasingly inclined to natural and healthy peptides to improve sleep. Herein, we isolated novel peptides with sleep-promoting ability from Pneumatophorus japonicus bone peptides (PBPs) and constructed an insomniac zebrafish model as a demonstration, incorporating behavioral and transcriptomic approaches to reveal the sleep-promoting effect and mechanism of PBPs. Specifically, a sequential targeting isolation approach was developed to refine and identify a peptide with remarkable sleep-promoting activity, namely TG7 (Tyr-Gly-Asn-Pro-Trp-Glu-Lys). TG7 shows comparable effects and a similar action pathway to melatonin in improving sleep. TG7 restores abnormal behavior of insomnia zebrafish to normal levels by upregulating the hnrnpa3 gene. The peptide downregulates per1b gene but upregulates cry1b, cry1ba and per2, improving the circadian rhythm. Furthermore, TG7 upregulates the genes gnb3b, arr3b and opn1mw1 to regulate the visual function. The above results indicate that TG7 improves circadian rhythms and attenuated abnormal alterations in visual function and motility induced by light, allowing for effective sleep promotion. This study isolated sleep-promoting peptides from PBPs, which provides a theoretical basis for the development of subsequent sleep-promoting products based on protein peptides.
A Novel Strain Breeding of Ganoderma lucidum UV119 (Agaricomycetes) with High Spores Yield and Strong Resistant Ability to Other Microbes’ Invasions
Authors: Tang Chuanhong , Tan Yi , Zhang Jingsong , Zhou Shuai , Honda Yoichi , Zhang Henan
Published: January 2023

The spore powder of Ganoderma lucidum (G. lucidum) has been proven to have a variety of pharmacological activities, and it has become a new resource for the development of health products and pharmaceuticals. However, the scarcity of natural resources, strict growth conditions and difficulty in controlling the stable yield, and quality of different culture batches seriously limit the development and utilization of G. lucidum spore powder. In the present study, the strain with the highest spore powder yield, G0109, was selected as the original strain to generate mutants of G. lucidum using ultraviolet ray irradiation. A total of 165 mutagenic strains were obtained, and fifty-five strains were chosen for the cultivation test. Importantly, one mutagenic strain with high spore powder yield and strong resistance to undesired microorganisms was acquired and named strain UV119. More cultivations demonstrated that the fruiting body and basidiospore yields from UV119 were, respectively, 8.67% and 19.27% higher than those of the parent (G0109), and the basidiospore yield was 20.56% higher than that of the current main cultivar “Longzhi No.1”. In conclusion, this study suggested that ultraviolet ray irradiation is an efficient and practical method for Ganoderma strain improvement and thus provided a basis for the development and application of G. lucidum spore production and outstanding contributions to the rapid development of the G. lucidum industry.
Characterizing Spray-Dried Powders through NIR Spectroscopy: Effect of Two Preparation Strategies for Calibration Samples and Comparison of Two Types of NIR Spectrometers
Authors: Tu Zhiyang (Stan) , Irudayaraj Joseph , Lee Youngsoo
Published: January 2023

Emerging portable near infrared (NIR) spectroscopic approaches coupled with data analysis and chemometric techniques provide opportunities for the rapid characterization of spray-dried products and process optimization. This study aimed to enhance the understanding of applying NIR spectroscopy in spray-dried samples by comparing two sample preparation strategies and two spectrometers. Two sets of whey protein–maltodextrin matrixes, one with a protein content gradient and one with a consistent protein content, were spray-dried, and the effect of the two preparation strategies on NIR calibration model development was studied. Secondly, a portable NIR spectrometer (PEAK) was compared with a benchtop NIR spectrometer (CARY) for the moisture analysis of prepared samples. When validating models with the samples with focused protein contents, the best PLS protein models established from the two sample sets had similar performances. When comparing two spectrometers, although CARY outperformed PEAK, PEAK still demonstrated reliable performance for moisture analysis, indicating that it is capable as an inline sensor.
Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences
Authors: Wang Mengyu , Wang Haoqian , Li Kai , Li Xiaoman , Wang Xujing , Wang Zhixing
Published: January 2023

Nowadays, with the rapid development of biotechnology, the CRISPR/Cas technology in particular has produced many new traits and products. Therefore, rapid and high-resolution detection methods for biotechnology products are urgently needed, which is extremely important for safety regulation. Recently, in addition to being gene editing tools, CRISPR/Cas systems have also been used in detection of various targets. CRISPR/Cas systems can be successfully used to detect nucleic acids, proteins, metal ions and others in combination with a variety of technologies, with great application prospects in the future. However, there are still some challenges need to be addressed. In this review, we will list some detection methods of genetically modified (GM) crops, gene-edited crops and single-nucleotide polymorphisms (SNPs) based on CRISPR/Cas systems, hoping to bring some inspiration or ideas to readers.
Approximate Controllability of Delayed Fractional Stochastic Differential Systems with Mixed Noise and Impulsive Effects
Authors: Hakkar Naima , Dhayal Rajesh , Debbouche Amar , Torres Delfim F. M.
Published: January 2023

We herein report a new class of impulsive fractional stochastic differential systems driven by mixed fractional Brownian motions with infinite delay and Hurst parameter H^∈(1/2,1). Using fixed point techniques, a q-resolvent family, and fractional calculus, we discuss the existence of a piecewise continuous mild solution for the proposed system. Moreover, under appropriate conditions, we investigate the approximate controllability of the considered system. Finally, the main results are demonstrated with an illustrative example.
Chitosan-Based Green Pea (Pisum sativum L.) Pod Extract Gel Film: Characterization and Application in Food Packaging
Authors: Elsebaie Essam Mohamed , Mousa Mona Metwally , Abulmeaty Samah Amin , Shaat Heba Ali Yousef , Elmeslamy Soher Abd-Elfttah , Asker Galila Ali , Faramawy Asmaa Antar , Shaat Hala Ali Yousef , Abd Elrahman Wesam Mohammed , Eldamaty Hanan Salah Eldeen , Abd Allah Amira Lotfy , Badr Mohamed Reda
Published: January 2023

This work focuses on studying the preparation, characterization (physical, mechanical, optical, and morphological properties as well as antioxidant and antimicrobial activities) and packaging application of chitosan (CH)-based gel films containing varying empty green pea pod extract (EPPE) concentrations (0, 1, 3, and 5% w/w). The experiments revealed that adding EPPE to CH increased the thickness (from 0.132 ± 0.08 to 0.216 ± 0.08 mm), density (from 1.13 ± 0.02 to 1.94 ± 0.02 g/cm3), and opacity (from 0.71 ± 0.02 to 1.23 ± 0.04), while decreasing the water vapour permeability, water solubility, oil absorption ratio, and whiteness index from 2.34 to 1.08 × 10−10 g−1 s−1 pa−1, from 29.40 ± 1.23 to 18.75 ± 1.94%, from 0.31 ± 0.006 to 0.08 ± 0.001%, and from 88.10 ± 0.43 to 77.53 ± 0.48, respectively. The EPPE films had better tensile strength (maximum of 26.87 ± 1.38 MPa), elongation percentage (maximum of 58.64 ± 3.00%), biodegradability (maximum of 48.61% after 3 weeks), and migration percentages than the pure CH-gel film. With the addition of EPPE, the antioxidant and antibacterial activity of the film improved. SEM revealed that as EPPE concentration increased, agglomerates formed within the films. Moreover, compared to control samples, packing corn oil in CH-based EPPE gel films slowed the rise of thiobarbituric acid and peroxide values. As an industrial application, CH-based EPPE films have the potential to be beneficial in food packaging.
Antioxidant, Anti-Inflammatory and Attenuating Intracellular Reactive Oxygen Species Activities of Nicotiana tabacum var. Virginia Leaf Extract Phytosomes and Shape Memory Gel Formulation
Authors: Chittasupho Chuda , Chaobankrang Kunyakorn , Sarawungkad Araya , Samee Weerasak , Singh Sudarshan , Hemsuwimon Kirachuda , Okonogi Siriporn , Kheawfu Kantaporn , Kiattisin Kanokwan , Chaiyana Wantida
Published: January 2023

Oxidative stress is one of the major causes of skin aging. In this study, the shape memory gels containing phytosomes were developed as a delivery system for Nicotiana tabacum var. Virginia fresh (VFL) and dry (VDL) leaf extracts. The extracts were loaded in the phytosomes by a solvent displacement method. The physical and chemical characteristics and stability of phytosomes were evaluated by dynamic light scattering and phytochemistry, respectively. The in vitro antioxidant activity and intracellular reactive oxygen species reduction of phytosomes and/or extracts were investigated by the DPPH and ABTS radical scavenging assays, FRAP assay, and DCFH-DA fluorescent probe. The cytotoxicity and anti-inflammatory activity of VDL and VFL phytosomes were studied by an MTT and a nitric oxide assay, respectively. Here, we first reported the total phenolic content in the dry leaf extract of N. tabacum var. Virginia was significantly greater than that of the fresh leaf extract. The HPLC analysis results revealed that VDL and VFL extracts contained 4.94 ± 0.04 and 3.13 ± 0.01 µg/mL of chlorogenic acid and 0.89 ± 0.00 and 0.24 ± 0.00 µg/mL of rutin, respectively. The phytosomes of the VDL and VFL extracts displayed stable size, polydispersity index, zeta potential values, and good chemical stability. VDL and VDL phytosomes showed higher phenolic and flavonoid contents which showed stronger DPPH and ABTS radical scavenging effects and reduced the intracellular ROS. The results suggested that the phenolic compounds are the main factor in their antioxidant activity. Both VDL and VFL phytosomes inhibited nitric oxide production induced by LPS, suggesting the anti-inflammatory activity of the phytosomes. The shape memory gel containing VDL and VFL phytosomes had good physical stability in terms of pH and viscosity. The VDL and VFL phytosomes dispersed in the shape memory gels can be considered as a promising therapeutic delivery system for protecting the skin from oxidation and reactive oxygen species.
Visualized Study on a New Preformed Particle Gels (PPG) + Polymer System to Enhance Oil Recovery by Oil Saturation Monitoring Online Flooding Experiment
Authors: Pi Yanfu , Liu Jinxin , Cao Ruibo , Liu Li , Ma Yingxue , Gu Xinyang , Li Xianjie , Fan Xinyu , Zhao Mingjia
Published: January 2023

After tertiary recovery from the oilfields, improving the production of the remaining hydrocarbon is always challenging. To significantly improve oil recovery, a heterogeneous composite flooding system has been developed with preformed particle gels (PPG) and polymers according to the technical approach of plugging and flooding combination. In addition, an oil saturation monitoring device and a large-scale 3D physical model were designed to better evaluate the performance of the technique. The evaluation results show that the viscosity, stability, and elasticity of the heterogeneous composite flooding system are better than the single polymer system. In addition, both systems exhibit pseudoplastic fluid characteristics and follow the principle of shear thinning. The results of seepage experiments showed that PPG migrates alternately in porous media in the manner of “piling plugging-pressure increasing-deformation migration”. The heterogeneous composite system can migrate to the depths of the oil layer, which improves the injection profile. In the visualization experiment, the heterogeneous composite system preferentially flowed into the high-permeability layer, which increased the seepage resistance and forced the subsequent fluid to flow into the medium and low permeability layers. The average saturation of the high, medium, and low permeability layers decreased by 4.74%, 9.51%, and 17.12%, respectively, and the recovery factor was further improved by 13.56% after the polymer flooding.
Specific Alcohol-Responsive Photonic Crystal Sensors Based on Host-Guest Recognition
Authors: Cai Xiaolu , Zhang Xiaojing , Fan Jing , Zheng Wenxiang , Zhang Tianyi , Qiu Lili , Meng Zihui
Published: January 2023

A photonic crystal material based on β-cyclodextrin (β-CD) with adsorption capacity is reported. The materials ((A-β-CD)-AM PC) consist of 3D poly (methyl methacrylate) (PMMA) colloidal microsphere arrays and hydrogels supplemented with β-cyclodextrin modified by acryloyl chloride. The prepared materials are then utilized for VOCs gas sensing. The 3D O-(A-β-CD)-AM PC was used to detect toluene, xylene, and acetone and the response was seen as the red-shift of the reflection peak. The 3D I-(A-β-CD)-AM PC was used to detect toluene, xylene, and acetone which occurred redshifted, while methanol, ethanol, and propanol and the peaks’ red-shifting was observed. However, among these, methanol gave the largest red-shift response The sensor has broad prospects in the detection of alcohol and the detection of alcohol-loaded drug releases in the future.
Editorial on Special Issue: “Smart Polymer Hydrogels: Synthesis, Properties and Applications—Volume I”
Authors: Ji Wei
Published: January 2023

Smart polymer hydrogels are soft materials formed by crosslinking with various covalent and non-covalent interactions [...]
Study of Energy Saving Using Silica Aerogel Insulation in a Residential Building
Authors: Thie Conal , Quallen Sean , Ibrahim Ahmed , Xing Tao , Johnson Brian
Published: January 2023

Energy consumption, specifically in the building sector, is expected to rise. One potential way to reduce energy consumption, or to slow this increase, is to reduce the heat loss in residential homes. Silica aerogels have grown in popularity as an insulating material due to their extremely low thermal conductivity. However, the benefits of using silica aerogels as an insulator in residential buildings have not been thoroughly studied. To understand the benefits of using silica aerogels as a thermal insulator in residential homes, experimentally validated simulations were performed. The simulations were performed on a model of a full-scale residential house using the multiphysics software ANSYS FLUENT 2019 R2. The simulations helped predict the actual saving benefits of using aerogels as an insulator. Aerogels have the potential to be used as an insulator in both the walls and windows due to its semitransparency. The results showed that the average kWh savings using one half-inch layer of wall aerogel insulation coupled with window aerogel insulation was 20.9% for the single-family house compared to traditional insulation. On average, the energy lost through the windows was 39.1% lower when using aerogel insulation compared to standard insulating materials. The energy lost through the house walls was 13.3% lower on average when using a thin layer of aerogel insulation. While a thin layer of aerogel insulation provided a benefit when used in the house walls, the potential for savings per quantity used was greater in the windows.
Framework of the Alu Subfamily Evolution in the Platyrrhine Three-Family Clade of Cebidae, Callithrichidae, and Aotidae
Authors: Storer Jessica , Walker Jerilyn , Baker Jasmine , Hossain Shifat , Roos Christian , Wheeler Travis , Batzer Mark
Published: January 2023

The history of Alu retroposons has been choreographed by the systematic accumulation of inherited diagnostic nucleotide substitutions to form discrete subfamilies, each having a distinct nucleotide consensus sequence. The oldest subfamily, AluJ, gave rise to AluS after the split between Strepsirrhini and what would become Catarrhini and Platyrrhini. The AluS lineage gave rise to AluY in catarrhines and to AluTa in platyrrhines. Platyrrhine Alu subfamilies Ta7, Ta10, and Ta15 were assigned names based on a standardized nomenclature. However, with the subsequent intensification of whole genome sequencing (WGS), large scale analyses to characterize Alu subfamilies using the program COSEG identified entire lineages of subfamilies simultaneously. The first platyrrhine genome with WGS, the common marmoset (Callithrix jacchus; [caljac3]), resulted in Alu subfamily names sf0 to sf94 in an arbitrary order. Although easily resolved by alignment of the consensus sequences, this naming convention can become increasingly confusing as more genomes are independently analyzed. In this study, we reported Alu subfamily characterization for the platyrrhine three-family clade of Cebidae, Callithrichidae, and Aotidae. We investigated one species/genome from each recognized family of Callithrichidae and Aotidae and of both subfamilies (Cebinae and Saimiriinae) of the family Cebidae. Furthermore, we constructed a comprehensive network of Alu subfamily evolution within the three-family clade of platyrrhines to provide a working framework for future research. Alu expansion in the three-family clade has been dominated by AluTa15 and its derivatives.
Molecular Characterization and Phylogenetic Analysis of Casein Gene Family in Camelus ferus
Authors: Parveen Shakeela , Zhu Peng , Shafique Laiba , Lan Hong , Xu Dingyun , Ashraf Sana , Ashraf Saba , Sherazi Maryam , Liu Qingyou
Published: January 2023

Camel milk is known for its exceptional medical uses. It has been used since ancient times to treat infant diarrhea, hepatitis, insulin-dependent diabetes (IDDM), lactose intolerance, alcohol-induced liver damage, allergies, and autism. It has the power to treat several diseases, with cancer being the most significant. This study investigated the evolutionary relationship, physiochemical characteristics, and comparative genomic analysis of the casein gene family (CSN1S1, CSN2, CSN1S2, and CSN3) in Camelus ferus. Molecular phylogenetics showing the camelid species clustered casein nucleotide sequences into four groups: CSN1S1, CSN2, CSN1S2, and CSN3. The casein proteins from camels were evaluated and found to be unstable, thermostable, and hydrophilic. CSN1S2, CSN2, and CSN3 were acidic, but CSN1S1 was basic. CSN1S1 showed positive selection for one amino acid (Q), CSN1S2 and CSN2 for three (T, K, Q), and CSN3 showed no positive selection. We also compared high-milk-output species such as cattle (Bos Tarus) and low-milk-yield species such as sheep (Ovies Aries) with camels (Camel ferus) and discovered that YY1 sites are more frequent in sheep than in camels and very low in cattle. We concluded that the ratio of YY1 sites in these species may affect milk production.
Parallel Evolution of Sex-Linked Genes across XX/XY and ZZ/ZW Sex Chromosome Systems in the Frog Glandirana rugosa
Authors: Mawaribuchi Shuuji , Ito Michihiko , Ogata Mitsuaki , Yoshimura Yuri , Miura Ikuo
Published: January 2023

Genetic sex-determination features male (XX/XY) or female heterogamety (ZZ/ZW). To identify similarities and differences in the molecular evolution of sex-linked genes between these systems, we directly compared the sex chromosome systems existing in the frog Glandirana rugosa. The heteromorphic X/Y and Z/W sex chromosomes were derived from chromosomes 7 (2n = 26). RNA-Seq, de novo assembly, and BLASTP analyses identified 766 sex-linked genes. These genes were classified into three different clusters (XW/YZ, XY/ZW, and XZ/YW) based on sequence identities between the chromosomes, probably reflecting each step of the sex chromosome evolutionary history. The nucleotide substitution per site was significantly higher in the Y- and Z-genes than in the X- and W- genes, indicating male-driven mutation. The ratio of nonsynonymous to synonymous nucleotide substitution rates was higher in the X- and W-genes than in the Y- and Z-genes, with a female bias. Allelic expression in gonad, brain, and muscle was significantly higher in the Y- and W-genes than in the X- and Z-genes, favoring heterogametic sex. The same set of sex-linked genes showed parallel evolution across the two distinct systems. In contrast, the unique genomic region of the sex chromosomes demonstrated a difference between the two systems, with even and extremely high expression ratios of W/Z and Y/X, respectively.
Functional Analyses of Rare Germline Missense BRCA1 Variants Located within and outside Protein Domains with Known Functions
Authors: Hovland Henrikke Nilsen , Mchaina Eunice Kabanyana , Høberg-Vetti Hildegunn , Ariansen Sarah Louise , Sjursen Wenche , Van Ghelue Marijke , Haukanes Bjørn Ivar , Knappskog Per Morten , Aukrust Ingvild , Ognedal Elisabet
Published: January 2023

The BRCA1 protein is implicated in numerous important cellular processes to prevent genomic instability and tumorigenesis, and pathogenic germline variants predispose carriers to hereditary breast and ovarian cancer (HBOC). Most functional studies of missense variants in BRCA1 focus on variants located within the Really Interesting New Gene (RING), coiled-coil and BRCA1 C-terminal (BRCT) domains, and several missense variants in these regions have been shown to be pathogenic. However, the majority of these studies focus on domain specific assays, and have been performed using isolated protein domains and not the full-length BRCA1 protein. Furthermore, it has been suggested that BRCA1 missense variants located outside domains with known function are of no functional importance, and could be classified as (likely) benign. However, very little is known about the role of the regions outside the well-established domains of BRCA1, and only a few functional studies of missense variants located within these regions have been published. In this study, we have, therefore, functionally evaluated the effect of 14 rare BRCA1 missense variants considered to be of uncertain clinical significance, of which 13 are located outside the well-established domains and one within the RING domain. In order to investigate the hypothesis stating that most BRCA1 variants located outside the known protein domains are benign and of no functional importance, multiple protein assays including protein expression and stability, subcellular localisation and protein interactions have been performed, utilising the full-length protein to better mimic the native state of the protein. Two variants located outside the known domains (p.Met297Val and p.Asp1152Asn) and one variant within the RING domain (p.Leu52Phe) were found to make the BRCA1 protein more prone to proteasome-mediated degradation. In addition, two variants (p.Leu1439Phe and p.Gly890Arg) also located outside known domains were found to have reduced protein stability compared to the wild type protein. These findings indicate that variants located outside the RING, BRCT and coiled-coiled domains could also affect the BRCA1 protein function. For the nine remaining variants, no significant effects on BRCA1 protein functions were observed. Based on this, a reclassification of seven variants from VUS to likely benign could be suggested.
Case Report: Rare IKZF1 Gene Fusions Identified in Neonate with Congenital KMT2A-Rearranged Acute Lymphoblastic Leukemia
Authors: Eadie Laura N. , Rehn Jacqueline A. , Breen James , Osborn Michael P. , Jessop Sophie , Downes Charlotte E. J. , Heatley Susan L. , McClure Barbara J. , Yeung David T. , Revesz Tamas , Saxon Benjamin , White Deborah L.
Published: January 2023

Chromosomal rearrangements involving the KMT2A gene occur frequently in acute lymphoblastic leukaemia (ALL). KMT2A-rearranged ALL (KMT2Ar ALL) has poor long-term survival rates and is the most common ALL subtype in infants less than 1 year of age. KMT2Ar ALL frequently occurs with additional chromosomal abnormalities including disruption of the IKZF1 gene, usually by exon deletion. Typically, KMT2Ar ALL in infants is accompanied by a limited number of cooperative le-sions. Here we report a case of aggressive infant KMT2Ar ALL harbouring additional rare IKZF1 gene fusions. Comprehensive genomic and transcriptomic analyses were performed on sequential samples. This report highlights the genomic complexity of this particular disease and describes the novel gene fusions IKZF1::TUT1 and KDM2A::IKZF1.
Exploring the Effect of Balanced and Imbalanced Multi-Class Distribution Data and Sampling Techniques on Fruit-Tree Crop Classification Using Different Machine Learning Classifiers
Authors: Chabalala Yingisani , Adam Elhadi , Ali Khalid Adem
Published: January 2023

Fruit-tree crops generate food and income for local households and contribute to South Africa’s gross domestic product. Timely and accurate phenotyping of fruit-tree crops is essential for innovating and achieving precision agriculture in the horticulture industry. Traditional methods for fruit-tree crop classification are time-consuming, costly, and often impossible to use for mapping heterogeneous horticulture systems. The application of remote sensing in smallholder agricultural landscapes is more promising. However, intercropping systems coupled with the presence of dispersed small agricultural fields that are characterized by common and uncommon crop types result in imbalanced samples, which may limit conventionally applied classification methods for phenotyping. This study assessed the influence of balanced and imbalanced multi-class distribution and data-sampling techniques on fruit-tree crop detection accuracy. Seven data samples were used as input to adaptive boosting (AdaBoost), gradient boosting (GB), random forest (RF), support vector machine (SVM), and eXtreme gradient boost (XGBoost) machine learning algorithms. A pixel-based approach was applied using Sentinel-2 (S2). The SVM algorithm produced the highest classification accuracy of 71%, compared with AdaBoost (67%), RF (65%), XGBoost (63%), and GB (62%), respectively. Individually, the majority of the crop types were classified with an F1 score of between 60% and 100%. In addition, the study assessed the effect of size and ratio of class imbalance in the training datasets on algorithms’ sensitiveness and stability. The results show that the highest classification accuracy of 71% could be achieved from an imbalanced training dataset containing only 60% of the original dataset. The results also showed that S2 data could be successfully used to map fruit-tree crops and provide valuable information for subtropical crop management and precision agriculture in heterogeneous horticultural landscapes.
Prospective Study on Geosciences On-Line Education: UNESCO Global Geoparks in Spain and Portugal
Authors: Martínez-Martín Jesús Enrique , Ester Mariñoso Pilar , Rosado-González Emmaline M. , Sá Artur A.
Published: January 2023

UNESCO Global Geoparks (UGGps) stand out as territories of excellence for the development of educational activities in the international arena. Their didactic potential, their multidisciplinarity and their importance for the development of non-formal and informal teaching activities have drawn the attention of institutions, organizations and governments of many countries. This, to such an extent, that the number of UGGps continues to increase year after year, having currently reached 177 territories spread over 46 countries. All of them work every day developing different activities and educational proposals aimed at the creation of the so-called “Quality Education”, the fourth objective of the SDGs of the 2030 Agenda. The diversity of didactic plans, their adaptability and their accessibility mean that each UGGp is unique and different from the rest, maintaining the key values that make up this group of territories. This study describes the different educational proposals and activities that the Spanish and Portuguese UGGps show on their official websites, with the aim of analyzing their level of visibility before visiting the territories and highlighting their relevance in the education development framework.
Cardiovascular Involvement of a CD138-Negative Anaplastic Myeloma: A Diagnostic Dilemma
Authors: Choudhuri Jui , Janmey Victor , Ding Juan , Dailey Denise , Shi Yang , Wang Yanhua
Published: January 2023

Anaplastic myeloma (AM) is an extremely rare and aggressive histological variant of myeloma. It is characterized by extramedullary presentation in the young and has a poor prognosis. It can be a diagnostic challenge when myeloma is not suspected and even more when the immunophenotype is unexpected. We present a rare presentation of anaplastic myeloma with cardiovascular involvement. Though the patient did not have the typical clinical features of myeloma, except lytic lesion in the femur, the cardiac biopsy showed sheets of anaplastic cells, and some with multinucleation. There were also some areas with a more plasmacytoid appearance. The initial immunohistochemical panel was negative for CD3, CD20, CD138, AE1/3, and kappa. It was positive for lambda. This led to an extended panel which showed positivity for CD79a and MUM1 and negative for LMP-1, HHV-8, CD43, CD117, CD56, and CD30. Even the flow cytometry on the bone marrow showed a small population of atypical cells positive for CD38 and negative for CD138 with lambda restriction. This is an unusual case of anaplastic myeloma with cardiovascular involvement and CD138 negativity. This case highlights the need to add a panel of plasma cell markers when myeloma is suspected, and it is pertinent to read flow cytometry with caution to avoid missing atypical plasma cells which maybe CD38+/CD138−.
JAK2 Mutations Are Rare and Diverse in Myelodysplastic Syndromes: Case Series and Review of the Literature
Authors: Delio Melissa , Bryke Christine , Mendez Lourdes , Joseph Loren , Jassim Sarmad
Published: January 2023

Objectives: To investigate and characterize JAK2 mutations in myelodysplastic syndrome (MDS), we present three cases with diverse JAK2 mutations and review the literature. Methods: The institutional SoftPath software was used to find MDS cases between January 2020 and April 2022. The cases with a diagnosis of a myelodysplastic/myeloproliferative overlap syndrome including MDS/MPN with ring sideroblasts and thrombocytosis were excluded. The cases with molecular data by next generation sequencing looking for gene aberrations commonly seen in myeloid neoplasms were reviewed for the detection of JAK2 mutations including variants. A literature review on the identification, characterization, and significance of JAK2 mutations in MDS was performed. Results: Among 107 cases of the MDS reviewed, a JAK2 mutation was present in three cases, representing 2.8% of the overall cases. A JAK2 V617F mutation was found in one case representing slightly less than 1% of all the MDS cases. In addition, we found JAK2 R564L and JAK2 I670V point mutation variants to be associated with a myelodysplastic phenotype. Conclusions: JAK2 mutations in MDS are rare and represent less than 3% of cases. It appears that JAK2 variant mutations in MDS are diverse and further studies are needed to understand their role in the phenotype and prognosis of the disease.
Cultivating the Future in Higher Education: Fostering Students’ Life-World Becoming with Wisdom Pedagogy
Authors: Jakubik Maria
Published: January 2023

The aim of this paper is to cultivate the future in Higher Education (HE), firstly by looking backward and learning from the past, then by looking around and questioning the present, and finally, by looking forward and imagining the future of HE. This paper seeks to answer the question of how HE can foster students’ life-world becoming, their emancipatory competence with wisdom pedagogy. The research method is based on selected literature from German educational philosophy (Herder, Humboldt, Hegel, Heidegger, and Gadamer) and on recent international publications discussing Bildung, self-cultivation, and life-world becoming in relation to HE. The findings show the need for moral education to enhance students’ flourishing in life with wisdom pedagogy. In the future, HE needs to focus more on cultivating character, emancipatory competence, life-world becoming, values, justice, trust, truth, and intellectual virtues such as intellectual humility, curiosity, open-mindedness, and courage. This paper offers a framework for synthesizing the epistemological and ontological goals of HE, and a framework that presents the place and role of wisdom pedagogy in developing emancipatory competences. This paper argues for applying wisdom pedagogy and its methods by teachers in HE to foster students’ capacity to flourish in life. The paper calls for more debates and research in understanding wisdom pedagogy in HE.
The Tenure Track Model: Its Acceptance and Perceived Gendered Character
Authors: O’Connor Pat , Drew Eileen
Published: January 2023

This article is concerned with the tenure track (TT) model, which has become increasingly used to extend the period of early career academics’ probation from one to five years across the EU. This article focuses on the TT in Trinity College Dublin (TCD), the oldest and most prestigious university in Ireland, one where gender equality has been embedded more consistently and where the pace of change has been faster than in other Irish universities. Drawing on interviews with thirteen men and women in three faculties, all but one of whom had successfully achieved tenure, this article explores their acceptance of the TT model and the perceived relevance of gender. Men were more likely to accept the model and less likely to see it as gendered. Even those women who identified a lack of clarity around maternity leave and/or gender differences in negotiating ‘fixed’ starting salaries did not identify a systemic gender issue but blamed themselves. Women who were ‘outsiders’ to TCD and in the arts, humanities and social science faculty were most likely to be critical of the model. The findings suggest the importance of a cautionary appraisal of TT, even in institutions that have actively sought to enhance gender equality.
Flowering Time and Physiological Reaction of Dendrobium nobile Lindl in Response to TDZ Application
Authors: Ren Shuxian , Hu Menglu , Wu Qian , Wang Lin , Gu Huaishan , Chen Ziyue , Ming Zhu , Li Zongyan
Published: January 2023

The objective of this work was to analyze the effect of Thidiazuron (TDZ) treatment on floral initiation, flowering time, ornamental characteristics and physiological metabolism of potted Dendrobium nobile. Three TDZ concentrations (200, 500 and 1000 mg L−1) were applied as solution to water the root zone of the plants. Control plants (plants watered with water) showed a good vegetative development but no floral branches. TDZ greatly influenced the flowering process. For all the tested TDZ concentrations, the first flower bud occurred at 55–60 days after the last irrigation (DAI), the highest TDZ concentration showing the major delay in its occurrence. The initial flowering (30% of flowered plants) began 47 days after the first flower bud initiation with no statistical differences among the treatments. Plants treated with TDZ 500–1000 mg L−1 showed the longest period of flowering (about 32 days) and the single flowers delayed the withering of about 2–3 days compared to the lowest TDZ treatment (200 mg L−1). The number of flowers, floral branches and flowering percentage were distinctly influenced by the TDZ concentration. The highest percentage of flowering (40%) was scored when plants were watered with a TDZ solution at 500 mg L−1 and this was a performant treatment providing the best morphological flower features for the ornamental value of this plant. Among the physiological factors affecting the flowering, this study showed that TDZ increased the relative membrane permeability which facilitated the transport of macromolecular flower-forming substances into and out of the membrane. Therefore, the membrane permeability change could be an indicator of shifts in physiologically active substances during the flowering transition process in Dendrobium nobile plants.