bluuebunny/crossref_metadata_2025 huggingface dataset:
Peningkatan Kompetensi Guru SMP Mata Pelajaran Bahasa Inggris di Jakarta Barat
Authors: Syamsudin Oom Rohmah , Hasbullah  , Suryana Andri , Suendarti Mamik
Published: July 2023

The purpose of this activity is to provide additional insight to teachers to meet the competency standards as professional teachers set by the government. Indonesian teachers can signify professional teachers if they require all 10 teacher competencies. The two qualifications they need to acquire are education and training and being able to plan and conduct the learning process according to their expertise. Abdimas team of Post Graduate Program Indraprasta University works hand in hand with the Center for Competency Development for Education and Vocational Educators of West Jakarta (P2KPTK2 Jakarta Barat) supporting the teachers to realize their goals of being professional teachers by accommodating an activity of short training in the pedagogic matter, especially in the difference of students characters as a basis for designing a lesson along with enrichment of English learning materials for junior high school level. The method used in this event is observation as well as the discussion with the teachers and P2KPTK2 concerning their needs in the topics mentioned. As a result of this online activity, teachers were very enthusiastic: this can be seen from the post-test score which was better than the pre-test score. Participants also hope that similar activities can be conducted on an ongoing basis as an update of their pedagogic and English skills.
Penggunaan Blended Learning di Masa Pandemi Covid-19: Kegiatan Pengabdian kepada Masyarakat pada Pesantren Terpadu Al-Musthafawiyah
Authors: Sulhan Muhammad , Anita Tri , Pujiyanto Rahmat
Published: July 2023

Pandemi covid-19 merupakan fenomena merebaknya wabah yang berimbas pada pola hidup masyarakat dunia. Imbar juga dirasakan pada dunia pendidikan yang dihadapkan pada penggunaan bauran pembelajaran. Penerapan pembelajaran blended learning dapat mengaktifkan peserta didik untuk terlibat dalam proses belajar-mengajar. Dengan blended learning juga, dapat melatih kemandirian peserta didik dalam belajar. Dengan menggunakan pembelajaran blended learning diharapkan guru dapat menguasai materi serta memiliki keterampilan yang berkaitan dengan teknologi sehingga lebih memudahkan dalam kegiatan belajar mengajar. Penggunaan flip pdf menjadikan materi dikemas lebih interaktif. Peserta didik dapat belajar secara mandiri tanpa tutor mulai dari membaca materi, mendalami materi, mengerjakan tugas, dan evaluasi secara mandiri seperti konsep PEDATI inginkan.
Pelatihan Batik Tie Dye kepada Kader Dasawisma sebagai Peningkatan Kreativitas
Authors: Rukiah Yayah , Susanti Khikmah , Saptodewo Febrianto
Published: July 2023

Batik yang kini telah menjadi identitas masyarakat Indonesia yang merupakan warisan budaya nenek moyang yang harus terus dilestarikan. Sebagaimana seperti yang diberitakan bahwa Batik sudah ditetapkan sebagai Indonesian Cultural Heritage, yaitu warisan budaya tak benda oleh United Nations Educational, Scientific and Cultural Organization (UNESCO) pada 2 Oktober 2009.  Oleh karenanya pengabdian masyarakat kali ini mengadakan pelatihan membatik, agar semua lapisan masyarakat turut membantu pelestarian batik.  Pelatihan batik kali ini adalah teknik batik jumputan yang lebih dikenal dengan sebutan Tie Dye, dimana batik yang dihasilkan melalui teknik ikat celup, teknik ini tidak begitu sulit karena tidak menggunakan lilin dan canting, sehingga siapa pun bisa melakukannya. Pelatihan membatik ini ditujukan untuk ibu-ibu Kader Dasawisma. Ibu-ibu Kader Dasawisma merupakan salah satu penggerak dan keberadaannya dapat menjadikan wadah bagi para ibu rumah tangga agar dapat menjadi perempuan yang aktif dan kreatif. Sehingga menjadikan masyarakat yang produktif. Hasil dari kegiatan pengabdian masyarakat ini, para peserta. Merasa sangat senang dan antusias terhadap pelatihan batik Tie Dye ini, selain itu peserta merasa mendapat keahlian baru yang sangat bermanfaat, dan bisa dijadikan keahlian untuk menciptakan peluang dalam meningkatkan perekonomian. Sebagai bagian dari masyarakat Indonesia, Kader Dasawisma memiliki peranan yang sangat penting dalam lingkungan, sehingga keberadaan nya di dalam masyarakat sangat dibutuhkan untuk penyebaran informasi, diharapkan dengan adanya pelatihan membatik ini, kreativitas peserta dapat digunakan untuk suatu hal yang positif.
Google Classroom sebagai Media Pembelajaran Daring di Masa Pandemik Covid-19
Authors: Saraswati Ria , Astri Indriana
Published: July 2023

Tujuan diadakannya kegiatan pengabdian masyarakat ini adalah untuk memberikan pelatihan penggunaan Google Classroom sebagai media pembelajaran daring. Google classroom adalah aplikasi yang dibuat oleh google yang bertujuan untuk membantu guru dan peserta didik apabila kedua hal tersebut berhalangan, mengorganisasi kelas serta berkomunikasi dengan mahapeserta didik tanpa harus terikat dengan jadwal sekolah di kelas. Disamping itu guru dapat memberikan tugas dan langsung memberikan nilai kepada siswa. Penyampaian pembelajaran dengan Google Classroom merupakan pembelajaran dengan memanfaatan teknologi internet untuk meningkatkan lingkungan belajar dengan konten yang kaya dengan cakupan yang luas. Memanfaatkan Google Classroom sebagai proses pembelajaran dapat membuat siswa sebagai partisipan mampu mengarsipkan tugas dan menghubungkannya dengan penyimpanan Google Drive. Fleksibilitas waktu dan tempat juga membuat Google Classroom menjadi proses pembelajaran yang disukai. Selain menjadikan siswa lebih mandiri juga membuat sesi diskusi layaknya kelas tatap muka tetap dapat terjalin. Kehadiran Google Classroom sebagai salah satu media pembelajaran diminati dan membuat pengalaman baru siswa dalam pembelajaran daring.
FREQUENCY OF IRON DEFICIENCY ANAEMIA AND ITS ASSOCIATION WITH PERSISTENT DIARRHOEA, LOW WEIGHT AND PARASITIC INFESTATION IN CHILDREN VISITING TEHSIL HEADQUARTER HOSPITAL DASKA.
Authors: Abid ali ranjha  , Hajira abid ranjha  , Riffat shafique  , Jawad ahmed  , S.shah hussain  , Omar Imran Butt@smcs.edu.pk  , Shizza ekhtisham  , Anum Imran Butt  , Abeer mustafa ranjha
Published: July 2023

Iron deficiency anemia (IDA) is a common health problem in Pakistan among younger children. Persistent diarrhea, one of the major causes of malnutrition in children especially under two years of age contributed to IDA resulting in increased mortality and morbidity in developing countries. The incidence and mortality is especially high during infancy in the absences of breast feeding. Number of factors effect prevalence of IDA in this age group, important ones, are low socio-economic status, low weight, worm infestation and persistent diarrhea.
PATIENTS SATISFACTION REGARDING CARE PROVISION IN MEDICAL OPD OF A TEACHING HOSPITAL OF SIALKOT
Authors: Akram Saeed  , Sahibzad Masood us Sayed  , Hassan Shahzad  , Mohammad Hamza Shahzad  , Momina Zahra  , Abaid Ur Rehman  , Balqees Fatima  , Faizan Haider Ali  , Kinza Fiaz  , Mahnoor Ali  , Minahil Nayyar  , Muhammad Waqar Ahmad  , Pakeeza Naeem  , Rameen Fatima  , Rumaisa Pervaiz  , SanaUllah  , Sawera Pervaiz  , Shiza Sagheer  , Syeda Khaula
Published: July 2023

The aim of the study is to evaluate the level of patient satisfaction about medical OPD services and accessibility of SMC Teaching Hospital and to find out the factors affecting patient satisfaction.
LEGAL ASPECTS OF THE CRC (CONVENTION ON RIGHTS OF CHILD-1989) AND IMPORTANT ARTICLES OF THE CONSTITUTION ISLAMIC REPUBLIC OF PAKISTAN, 1973) RELATED TO HUMAN AND CHILD RIGHTS
Authors: Admin Prof. Dr. Sahibzada Masood Us Syed
Published: July 2023

In this article you will find 41 Articles of UN CRC 1989 and their legal aspect in Pakistani Laws. We have made laws in majority of cases but the problem lies with implementation. You will find here some important Articles of Constitution of Pakistan 1973, there of pertaining to human and children rights, where constitution protects, the children rights.

In 2023, this article is revised according to the updated formation of different laws in Pakistan regarding Human (Children) Rights till todate (May, 2023).
KAP STUDY OF EATING JUNK FOOD WITH REFERENCE TO BMI AMONG STUDENTS OF SIALKOT MEDICAL COLLEGE (SMC), SIALKOT
Authors: Dr. Tahir Mahmood Butt1  , Muhammad Hassan Shahzad  , Muhammad Hamza Shahzad  , Adnan Iqbal  , Horaira Amjad  , Danish Kamal  , Abdur Rehman  , Fahad Ishtiaq  , Saad Suleman  , Syeda Haya Fatima  , Dua Ali  , Safoora Choudhry  , Sadeema Choudhry  , Khadija Iftikhar  , Tooba Ikram
Published: July 2023

To assess the eating habits of students and their effect on BMI along with other activities like exercise
L’espace carcéral comme lieu d’évasion dans Riz noir d’Anna Moï
Authors: Kim-Bernard Kyeongmi
Published: July 2023

Dans cette étude, j’explore les descriptions des espaces clos dans le roman Riz noir d’Anna Moï, dont la trame principale se déroule pendant quelques mois après le déclenchement de l’Offensives du Têt au Vietnam. L’imaginaire de la narratrice autodiégétique âgée de 15 ans flâne constamment entre deux espaces opposés par leur fonction : sa maison bourgeoise protégée de ce qui se passe à l’extérieur en plein milieu des tueries violentes et l’espace clos d’une cellule de prison nommée « la cage aux tigres » dans laquelle elle passe dix-sept mois. C’est dans ce dernier espace carcéral que le récit prend forme à l’aide de multiples réminiscences. Les constants va-et-vient entre deux espaces clos, l’un accueillant et l’autre hostile, que la jeune prisonnière fréquente avec autant d’obsession, se confondent en un seul lieu au moment du dénouement inattendu du récit. Ce rapprochement des deux lieux séparés arrive notamment lorsque le lecteur découvre l’allusion à ce qui s’est passé dans la vie de la jeune fille dans son domicile jusqu’à son emprisonnement. En m’appuyant sur l’analyse thématique, je tente de mettre en lumière comment et pourquoi cet espace carcéral devient un lieu d’introspection qui va la sauver paradoxalement de son isolement de l’extérieur en devenant un moyen de s’évader de son abîme intérieur.
« L’immeuble que nous sommes » : la réfraction de l’espace dans le Journal de confinement de Wajdi Mouawad
Authors: Brignoli Laura
Published: July 2023

L’étude se focalise sur le Journal du confinement, monologues que l’écrivain libanais d’expression française, Wajdi Mouawad, a diffusés en ligne au moment où le confinement dû à la pandémie a interrompu son activité théâtrale. On y analyse le rapport à l’espace qu’entretiennent quelques-uns des monologues, centrés spécifiquement sur la dimension de la demeure humaine, et sur le dialogue entre l’intérieur et l’extérieur, entre le moi envisagé sous l’angle du logement et l’univers perçu dans la perspective spatio- temporelle. Par le biais de l’enseignement bachelardien, on relève la fonction des espaces les plus porteurs de la signification globale de l’œuvre, en considérant, à travers les choix lexicaux, les effets d’écho, la récurrence des lieux, des thèmes ou des motifs, les valeurs sémantiques que l’auteur leur attribue, pour déterminer la manière dont le confinement a modifié la relation de l’auteur avec son propre espace et les moyens qu’il a trouvés pour sortir de la « cage ».
Louis Wolfson et la traduction plurilinguistique, ou comment se mettre à l’abri dans l’espace rhizomique
Authors: Hauck Nicholas
Published: July 2023

Cet article examine la traduction plurilinguistique de Louis Wolfson telle qu’elle est théorisée dans Le Schizo et les langues (1970) et l’expérience de la mise en pratique du système telle que Wolfson la raconte dans Ma mère, musicienne… (1984). Diagnostiqué comme schizophrène, Wolfson cultivait une haine profonde pour l’anglais, sa langue maternelle, et une forte méfiance envers les gens et le monde en général. Afin de se protéger, il apprend le français, le russe, l’allemand et l’hébreu, des langues qu’il utilise par la suite pour traduire son environnement anglophone. À partir des écrits de Caroline Rabourdin, qui démontrent que la logique de l’espace euclidien forme et informe notre identité linguistique – ce qu’elle appelle un bilinguisme incarné –, on arrive à voir que l'espace wolfsonien suit une autre logique, non-binaire, structurée sur la base de ses traductions plurilinguistiques. Pour Deleuze et Guattari, le système de Wolfson suit des lignes de fuite et fonctionne selon une reformulation de l’espace qui ressemble à celui du rhizome: non-hiérarchique et non-signifiant. Ainsi, chez Wolfson, le nomade remplace le flâneur, et les jeux de mots et la polysémie remplacent la signification pour créer un espace sans cesse traduit, le rendant enfin, mais temporairement, habitable.
L’espace dans l’écriture biographique de Roger Laporte
Authors: Cambria Domenico
Published: July 2023

Cet article permet de découvrir l'écriture biographique de Roger Laporte, notamment son lien avec l'espace qui en montre le développement. Cette écriture particulière que nous étudions est vécue seulement pendant l'acte d'écrire et son parcours se réalise dans le livre. Celui-ci est le lieu où l'écrivain peut revenir sur le lien entre son être en tant qu’écrivain et l'humain avec lequel il s'est confronté tout au long du chemin. Cela nous révèle un processus de spatialisation de l'écriture à travers le livre, en particulier l'insertion du lecteur, la réaction de l'écrivain face à ce tout complet qu'il ne domine pas et la relation entre ceux-ci qui s'y déroule.
L’objet monétaire comme objet romanesque : le cas de Balzac
Authors: Spandri Francesco
Published: July 2023

Le roman du XIXe siècle prête à l’objet monétaire une attention qui dépasse la simple évocation épisodique : conscient de la charge affective et métaphysique qu’elle véhicule, le romancier décrit la réalité concrète de l’équivalent général, il interroge ses virtualités et sa force symbolique. Dans La Comédie humaine, la monnaie métallique joue un rôle déterminant dans les transactions financières qui ont lieu entre les personnages. Mais le rôle de la monnaie fiduciaire est tout aussi significatif. La coexistence entre métal et papier véhicule l’idée d’une vitalité multiforme de la richesse et celle d’un rapport multiple à la matérialité de l’argent. Saisie dans sa masse ou dans son insignifiance apparente, la monnaie balzacienne devient élément sensoriel, fétiche qui déclenche le désir. Dissocié de sa valeur d’échange, l’objet monétaire prend vie et se mue de manière paradoxale en symbole de contestation de l’ordre économique.
En voyage avec François Villon et Sainte-Beuve
Authors: Mus David
Published: July 2023

Un certain nombre d'erreurs importantes dans notre lecture du Testament de François Villon ont eu pour conséquence de transformer ce grand poème en document autobiographique. Le résultat a été – pendant deux siècles – de dévoyer notre étude de ce poète vers la constitution d'une biographie largement fictive de l'auteur. Ainsi que de fausser, dans le long terme et grâce à l'influence de Sainte-Beuve, toute notre lecture de la poésie moderne en France. Ces erreurs dénoncées, dès 1967, n'ont pas changé grand- chose pour les éditeurs et exégètes de cette poésie, qui continuent de méconnaître, dans leurs travaux, les spécificités de l'art poétique.
Inteligencia artificial: en el ojo del huracán
Authors: Gibert Karina
Published: July 2023

timamente, levantarse y oír hablar de inteligencia artificial es un todo. Parece que no exista otra cosa de la que ocuparse en todos los foros posibles. La verdad es que los espectaculares avances que ha realizado esta disciplina recientemente han impactado en la sociedad, en la economía y en todos los ámbitos imaginables de una forma muy significativa, pero... ¿por qué de repente todo el mundo habla de ello?
COVID-19: respuestas desde la ingeniería y la inteligencia artificial
Authors: Salamea Palacios Christian
Published: July 2023

¿Cómo actúa nuestro cuerpo para defenderse? ¿Cómo funcionan las vacunas? ¿Cómo se diagnostica la enfermedad? ¿Puede la ingeniería y la inteligencia artificial dar una respuesta al diagnóstico? Estas preguntas sirvieron de base para el desarrollo de una investigación que permitió evaluar la respuesta de la inteligencia artificial frente a la pandemia. En estas páginas se describen conceptos fundamentales que se utilizaron para reconocer patrones relacionados con la tos COVID y de las capacidades reales de los sistemas inteligentes para detectarla. Esta obra tiene un enfoque de difusión científica, con un lenguaje cercano y amigable para los lectores y contribuye a los avances logrados alrededor del mundo.
Functional Bandaging in Children with Idiopathic Toe-Walking
Authors: Tuncer Deniz , Akalan N. Ekin , Caliskan M. Mine , Temelli Yener , Yigit Pakize
Published: July 2023

Background: Idiopathic toe-walking (ITW) is a persistent gait pattern with no known etiology characterized as premature heel rise or no heel contact. We investigated the effects of functional bandaging in children with ITW on heel contact during stance phase and on gait quality.

Methods: Nineteen children aged 4 to 16 years with ITW and ten age-matched healthy children were included in the study. Elastic adhesive bandages were applied to children with ITW to assist with dorsiflexion. Before bandaging (T0) and immediately (T1) and 1 week (T2) after initial bandaging, the initial contact, loading response, and midstance subphases of gait were analyzed using light pressure sensors and the Edinburgh Visual Gait Score (EVGS). Ten age-matched children with typical gait participated for comparison in T0. The data were analyzed with Friedman and Wilcoxon signed rank tests for within-group comparisons and Mann-Whitney U tests for between-group comparisons.

Results: In T0, for the ITW group, no heel contact was observed during stance. In T1, all of the participants achieved heel contact at initial contact and loading response and 56.8% at midstance. In T2, all of the heels continued contact at initial contact and loading response and 54.3% at midstance. The EVGS significantly improved. The Friedman test showed that there were noteworthy improvements between T0-T1 and T0-T2 in video-based observational gait analysis and EVGSs (P < .001), although no difference was found between T1-T2 in video-based observational gait analysis (P = .913) and EVGSs (P = .450).

Conclusions: In children with ITW, dorsiflexion assistive functional bandaging was an effective tool to help achieve heel contact on the ground and improve walking quality for a short period after application. Further studies with longer follow-up and larger sample sizes are required to confirm the long-term therapeutic effects of this promising functional bandaging.
Tillaux Fracture Associated with Adult Ankle Fracture Injury
Authors: Tran Alina T. , Nutter Kelsi , Singleton Tanya , Nguyen Tan , Lee Daniel
Published: July 2023

Tillaux fractures in adults are rare and, if unrecognized, can lead to ankle fracture healing complications, early progression of arthritis, and limited ankle movement caused by pain and degenerative changes. The Tillaux fracture was first described by Paul Tillaux as an external rotation injury of the ankle, involving an avulsion fracture of the distal anterolateral tibia. This fracture can be easily overlooked on plain radiographs in the adult. A high index of suspicion for this type of fracture pattern along with the use of computed tomographic scanning can help confirm the suspected diagnosis, rule out other tibial injuries, and provide more information on the best course of action. Historically, Tillaux fractures have been more common in adolescents because of the open tibial epiphyseal plate. Once the epiphyseal plate fully closes, skeletal maturity is achieved, thus making it extremely unusual for the anterior tibiofibular ligament to cause an avulsion fragment of the distal anterolateral tibia. Because of how uncommon this type of fracture is in adults, it has rarely been reported in our literature. We reviewed the literature and present a case report of this rare fracture injury.
Hallux Valgus Plantar Pressure Distribution Before and After Surgery
Authors: Mazzotti Antonio , Arceri Alberto , Martini Beatrice , Bonelli Simone , Zielli Simone , Artioli Elena , Brognara Lorenzo , Faldini Cesare
Published: July 2023

Hallux valgus is a common foot deformity that may cause pain and functional limitation, and often requires surgical correction. Clinical and radiographic parameters are typically used to assess postoperative outcomes. Plantar pressure distribution systems represent an innovative additional tool to evaluate hallux functional outcome after surgery. A systematic review of the current literature was performed to assess evaluation systems used for plantar pressure analysis and differences before and after hallux valgus surgery, and a possible relationship between different surgical techniques and clinical and radiographic results. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were used for this review. Initial search results yielded 40 studies. Two additional studies were found through cross-reference. Twenty-five studies were screened. A total of 10 articles were included in the review process. Two main plantar pressure analysis systems were identified. Hallux function restoration based on plantar pressure measurement did not always occur. No relevant relationships between plantar pressure distribution data and different surgical techniques were established. All patients achieved satisfactory clinical and radiographic outcomes, regardless of surgical techniques used; however, no clear relationships were observed between clinical and radiographic results and the change in foot plantar pressure patterns. The current literature on this topic showed several methodologic limitations. Therefore, it is not possible to provide sufficiently supported evidence-based data regarding plantar pressure distribution rebalance after surgery using current plantar pressure analysis systems. Further investigations are needed to fill these gaps in evidence.
Embryo-derive TNF promotes decidualization via fibroblast activation
Authors: Chen Si-Ting , Shi Wen-Wen , Lin Yu-Qian , Yang Zhen-Shan , Wang Ying , Li Meng-Yuan , Li Yue , Liu Ai-Xia , Hu Yali , Yang Zeng-Ming
Published: July 2023

Decidualization is a process in which endometrial stromal fibroblasts differentiate into specialized secretory decidual cells and essential for the successful establishment of pregnancy. The underlying mechanism during decidualization still remains poorly defined. Because decidualization and fibroblast activation share similar characteristics, this study was to examine whether fibroblast activation is involved in decidualization. In our study, fibroblast activation-related markers are obviously detected in pregnant decidua and under in vitro decidualization. ACTIVIN A secreted under fibroblast activation promotes in vitro decidualization. We showed that arachidonic acid released from uterine luminal epithelium can induce fibroblast activation and decidualization through PGI2 and its nuclear receptor PPARδ. Based on the significant difference of fibroblast activation-related markers between pregnant and pseudopregnant mice, we found that embryo-derived TNF promotes CPLA2α phosphorylation and arachidonic acid release from luminal epithelium. Fibroblast activation is also detected under human in vitro decidualization. Similar arachidonic acid-PGI2-PPARδ-ACTIVIN A pathway is conserved in human endometrium. Collectively, our data indicate that embryo-derived TNF promotes CPLA2α phosphorylation and arachidonic acid release from luminal epithelium to induce fibroblast activation and decidualization.
Control of telomere length in yeast by SUMOylated PCNA and the Elg1 PCNA unloader
Authors: Singh Pragyan , Gazy Inbal , Kupiec Martin
Published: July 2023

Telomeres cap and protect the linear eukaryotic chromosomes. Telomere length is determined by an equilibrium between positive and negative regulators of telomerase activity. A systematic screen for yeast mutants that affect telomere length maintenance in the yeast
Saccharomyces cerevisiae
revealed that mutations in any of ∼500 genes affects telomere length. One of the genes that, when mutated, causes telomere elongation is
ELG1
, which encodes an unloader of PCNA, the processivity factor for replicative DNA polymerases. PCNA can undergo SUMOylation on two conserved residues, K164 and K127, or ubiquitination at lysine 164. These modifications have already been implicated in genome stability processes. We report that SUMOylated PCNA acts as a signal that positively regulates telomerase activity. We also uncovered physical interactions between Elg1 and the CST (Cdc13-Stn1-Ten) complex, and dissected the mechanism by which Elg1 and Stn1 negatively regulates telomere elongation, coordinated by SUMO. We present a model that provides mechanistic insights on how chromosomal replication and telomere elongation are coordinated.
Folding of prestin’s anion-binding site and the mechanism of outer hair cell electromotility
Authors: Lin Xiaoxuan , Haller Patrick R , Bavi Navid , Faruk Nabil , Perozo Eduardo , Sosnick Tobin R
Published: July 2023

Prestin responds to transmembrane voltage fluctuations by changing its cross-sectional area, a process underlying the electromotility of outer hair cells and cochlear amplification. Prestin belongs to the SLC26 family of anion transporters yet is the only member capable of displaying electromotility. Prestin’s voltage-dependent conformational changes are driven by the putative displacement of residue R399 and a set of sparse charged residues within the transmembrane domain, following the binding of a Cl− anion at a conserved binding site formed by the amino termini of the TM3 and TM10 helices. However, a major conundrum arises as to how an anion that binds in proximity to a positive charge (R399), can promote the voltage sensitivity of prestin. Using hydrogen–deuterium exchange mass spectrometry, we find that prestin displays an unstable anion-binding site, where folding of the amino termini of TM3 and TM10 is coupled to Cl− binding. This event shortens the TM3–TM10 electrostatic gap, thereby connecting the two helices, resulting in reduced cross-sectional area. These folding events upon anion binding are absent in SLC26A9, a non-electromotile transporter closely related to prestin. Dynamics of prestin embedded in a lipid bilayer closely match that in detergent micelle, except for a destabilized lipid-facing helix TM6 that is critical to prestin’s mechanical expansion. We observe helix fraying at prestin’s anion-binding site but cooperative unfolding of multiple lipid-facing helices, features that may promote prestin’s fast electromechanical rearrangements. These results highlight a novel role of the folding equilibrium of the anion-binding site, and help define prestin’s unique voltage-sensing mechanism and electromotility.
Integrative species delimitation in the common ophiuroid Ophiothrix angulata (Echinodermata: Ophiuroidea): insights from COI, ITS2, arm coloration, and geometric morphometrics
Authors: Hernández-Díaz Yoalli Quetzalli , Solis Francisco , Beltrán-López Rosa G. , Benítez Hugo A. , Díaz-Jaimes Píndaro , Paulay Gustav
Published: July 2023

Ophiothrix angulata (Say, 1825) is one of the most common and well-known ophiuroids in the Western Atlantic, with a wide geographic and bathymetric range. The taxonomy of this species has been controversial for a century because of its high morphological variability. Here we integrate information from DNA sequence data, color patterns, and geometric morphometrics to assess species delimitation and geographic differentiation in O. angulata. We found three deeply divergent mtDNA-COI clades (K2P 17.0–27.9%). ITS2 nuclear gene and geometric morphometrics of dorsal and ventral arm plates differentiate one of these lineages, as do integrative species delineation analyses, making this a confirmed candidate species.
Genetic diversity of the breeding collection of tomato varieties in Kazakhstan assessed using SSR, SCAR and CAPS markers
Authors: Pozharskiy Alexandr , Kostyukova Valeriya , Khusnitdinova Marina , Adilbayeva Kamila , Nizamdinova Gulnaz , Kapytina Anastasiya , Kerimbek Nazym , Taskuzhina Aisha , Kolchenko Mariya , Abdrakhmanova Aisha , Kisselyova Nina , Kalendar Ruslan , Gritsenko Dilyara
Published: July 2023

Tomato is one of the most prominent crops in global horticulture and an important vegetable crop in Kazakhstan. The lack of data on the genetic background of local varieties limits the development of tomato breeding in the country. This study aimed to perform an initial evaluation of the breeding collection of tomato varieties from the point of view of their genetic structure and pathogen resistance using a set of PCR based molecular markers, including 13 SSR markers for genetic structure analysis, and 14 SCAR and CAPS markers associated with resistance to five pathogens: three viruses, fungus Fusarium oxysporum, and oomycete P hytophthora infestans. Nine SSR markers were with a PIC value varying from 0.0562 (low information content) to 0.629 (high information content). A weak genetic structure was revealed in the samples of varieties including local cultivars and, predominantly, varieties from Russia and other ex-USSR countries. The local varieties were closely related to several groups of cultivars of Russian origin. Screening for a set of resistance markers revealed the common occurrence of the resistance locus I against Fusarium oxysporum and only the occasional presence of resistance alleles of other markers. No markers of resistance to the three considered viruses were revealed in local tomato varieties. Only two local cultivars had markers of resistance to P. infestans, and only the ‘Meruert’ cultivar had a combination of resistance markers against P. infestans and F. oxysporum. The obtained results have demonstrated the need for further studies of local tomato varieties with a wider range of molecular markers and source germplasm to lay a foundation for the development of tomato breeding in Kazakhstan.
Identification of PIMREG as a novel prognostic signature in breast cancer via integrated bioinformatics analysis and experimental validation
Authors: Zhao Wenjing , Chang Yuanjin , Wu Zhaoye , Jiang Xiaofan , Li Yong , Xie Ruijin , Fu Deyuan , Sun Chenyu , Gao Ju
Published: July 2023

Background

Phosphatidylinositol binding clathrin assembly protein interacting mitotic regulator (PIMREG) expression is upregulated in a variety of cancers. However, its potential role in breast cancer (BC) remains uncertain.

Methods

The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to gather relevant information. The expression of PIMREG and its clinical implication in BC were assessed by using Wilcoxon rank-sum test. The prognostic value of PIMREG in BC was evaluated through the Cox regression model and nomogram, and visualized by Kaplan-Meier survival curves. Genes/proteins that interact with PIMREG in BC were also identified through GeneMANIA and MaxLink. Gene set enrichment analysis (GSEA) was then performed. The correlations of the immune cell infiltration and immune checkpoints with the expression of PIMREG in BC were explored via TIMER, TISIDB, and GEPIA. Potential drugs that interact with PIMREG in BC were explored via Q-omic. The siRNA transfection, CCK-8, and transwell migration assay were conducted to explore the function of PIMREG in cell proliferation and migration.

Results

PIMREG expression was significantly higher in infiltrating ductal carcinoma, estrogen receptor negative BC, and progestin receptor negative BC. High expression of PIMREG was associated with poor overall survival, disease-specific survival, and progression-free interval. A nomogram based on PIMREG was developed with a satisfactory prognostic value. PIMREG also had a high diagnostic ability, with an area under the curve of 0.940. Its correlations with several immunomodulators were also observed. Immune checkpoint CTLA-4 was significantly positively associated with PIMREG. HDAC2 was found as a potentially critical link between PIMREG and BRCA1/2. In addition, PIMREG knockdown could inhibit cell proliferation and migration in BC.

Conclusions

The high expression of PIMREG is associated with poor prognosis and immune checkpoints in BC. HDAC2 may be a critical link between PIMREG and BRCA1/2, potentially a therapeutic target.
Food Safety and Hygiene Practices in Medium Size Hotel in Ondo State, Nigeria
Authors: Oladeji S. O. , Arogundade C. , Alabi A. O. , Ijose O. A.
Published: July 2023

The increasing rate of diseases associated to food has called for serious attention.  This assertion forms the basis for undertaken this study that hinged on ensuring food safety and hygienic practices in medium size hotels in Ondo State, Nigeria. The study State was divided into three clusters and two hotels were randomly selected in each of the cluster. A field observation was undertaken to assess the level of compliance with standard guidelines on hygiene and safety measures, examining the equipment used in food preparations and safety measures. The food handlers in the selected hotels were randomly selected while visitors were purposively selected based on their willingness to participate in the study. A well-designed questionnaire was utilized to gather information from both tourists and food handlers. Microbial laboratory analysis was carried out on the collected food samples. The perceptions of the customers on the cleanliness and hygiene examined using a set of ten indicators revealed that customers had positive and good perception of the hotels’ environment (weighted mean =4.51 to 4.92). The results revealed that on the average the food handlers observed most of the itemized practices (weighted mean= 3.28 to 4.0). The viable count (TVC)/ Total viable bacteria count (cfu/g) ranged between (4-1)×103cfu/g. Though, the selected hotels adhered to food safety and hygiene practices, there is need to maintain and sustain this high level of performance by the relevant stakeholders.
Drivers’ Behaviour and Risk Perception among Haulage Companies in Rivers State, Nigeria
Authors: Asomaku S. O. , Arokoyu S. B. , Lawal O.
Published: July 2023

The study examined the risk perception influence on drivers’ behaviour (violations) among haulage companies in Rivers State, Nigeria. A total of 210 copies of questionnaire was administered among 20 haulage companies and the retrieved questionnaire were analysed using descriptive and inferential statistics such as frequency counts, percentage and spearman rank. The findings revealed that haulage drivers hardly ever involve in violations such as “pull out of a junction so far that the driver with the right of way has to stop and let you out” (41.4%), “stay in a motorway lane that you know will be closed ahead until the last minute before forcing your way into the other lane” (67.6%) and never disregarded the speed limit on a residential road (70.7%); however, they occasionally drive so close to the car in front that it would be difficult to stop in an emergency (51.0%). From the spearman rank analysis, it was revealed that there is a statistically significant relationship between the Risk Perception Index (RP_Index) and Ordinary Violation (OV_index) (where p-value < 0.05 and p = 0.000). The correlation coefficient (r) of the analysis showed that the relationship between RV_Index and AV_index is weak and positively correlated (were r = 0.256). It was concluded that the extent of risk perception by the haulage drivers have significant relationship with their violation behaviour, and such relationship tends to be weak and positive.
Assessment of the Oxidative Stress Status in those with Diabetes and Hypertension Attending Jos University Teaching Hospital (JUTH), Nigeria
Authors: Blessing Obot Uduakobong , Abigail Adugbe , Mariam Solomon D. , Samuel Gazuwa Y.
Published: July 2023

Diabetes is a serious, long-term condition with a major impact on the lives and wellbeing of individuals, families, and societies worldwide. This study aimed to investigate the impact of oxidative stress on individuals with type 2 diabetes mellitus (T2DM) and hypertension who were receiving care at the Endocrinology unit of Jos University Teaching Hospital (JUTH). A total of 54 subjects, comprising both males and females, were randomly selected for the study. The subjects included 27 patients diagnosed with T2DM and hypertension, as well as 27 healthy volunteers who were recruited as a control group. The study focused on assessing various parameters related to oxidative stress and antioxidant enzyme activity. The evaluation of oxidant status involved measuring Malondialdehyde (MDA) levels, which serve as a marker for lipid peroxidation, as well as Glycated hemoglobin (HbA1c) levels and Lipid profile. Additionally, the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH), was examined. Demographic information, lifestyle factors, and clinical assessments were also collected during the study. The results showed that HbA1c levels were significantly higher in diabetic patients compared to the healthy participants. Furthermore, the diabetic group exhibited higher MDA activity, indicating increased lipid peroxidation, compared to the control group (P = 0.001). In terms of antioxidant enzyme activity, the diabetic group showed elevated levels of SOD, CAT, and GSH compared to the control group (P = 0.001). Regarding lifestyle factors, there was a significant difference (P = 0.001) in lipid profiles between diabetic and non-diabetic individuals who engaged in regular exercise compared to those who did not exercise. Similarly, diabetic individuals who had consultations with a registered dietician or an Endocrinologist also demonstrated significantly improved lipid profiles compared to those who did not seek professional advice. Notably, diabetics who exercised regularly and received dietary guidance exhibited significantly higher levels of HDL compared to those who did not engage in exercise or receive dietary counseling. In conclusion, this study highlighted the association between oxidative stress and T2DM with hypertension. The findings emphasized the importance of managing oxidative stress through lifestyle modifications, such as regular exercise and dietary interventions, in improving lipid profiles and potentially mitigating the impact of diabetes on individuals' health and well-being.
Integration of AI for Routine Tasks Using Salesforce
Authors: Kaliuta Kiryl
Published: July 2023

Aims: In the previous decade, developments in machine learning techniques have drawn interest from the literature and corporate organizations to artificial intelligence technologies. However, despite the immense promise of AI technology for problem resolution, there are still limitations with practical application and a lack of expertise in employing AI strategically to produce business benefits. Customer Relationship Management (CRM) has recently experienced substantial developments. Companies have implemented AI-based CRM to effectively react to client inquiries and increase customer loyalty.

Study design: Qualitative analysis.

Methodology: We have selected 15 publications from the research database for further investigation. The rapid growth of modern sales technology literature has resulted in a rich but fragmented representation of what sales technology is, raising the question of how its position within the sales process can be effectively defined.

Results: The results of the literature review enabled the author to recognize three major subfields of AI literature within the CRM domain (AI and machine learning techniques used for CRM activities, strategic management of AI-CRM integrations, and AI integration in Salesforce) and gather promising future development paths for each of these subfields. This study also proposes a three-step theoretical framework for AI deployment in CRM, which may help scholars further improve their expertise in this sector and managers create a suitable and consistent approach.

Conclusion: A conceptual framework is offered, with four sources of value creation discussed: (i) decision assistance; (ii) consumer and employee involvement; (iii) automation; and (iv) new services and products. These findings add to both conceptual and administrative views, with several prospects for developing new theories and management techniques.
Investigating Pre-service Mathematics Teachers’ Perception on Cognitive Needs
Authors: Owusu-Ansah Nana Akosua , Apawu Jones
Published: July 2023

This article reports on part of a two- year study. The paper examined the perception of pre-service mathematics teachers’ cognitive needs via the lens of Maslow theory of needs. The research design employed was the mixed methods approach specifically sequential explanation mixed methods design. The population for this study was pre-service mathematics teachers at the University of Education, Winneba of Ghana. The purposive sampling technique was employed to select Level 300 pre-service mathematics teachers. Level 300 students were 183. The simple random sampling technique was used in selecting 125 respondents for the study. Questionnaire and interview protocol were used as instruments to collect data. Data collected through the questionnaire were analysed quantitatively and the interview data collected were analysed qualitatively. Results showed that the cognitive needs of the pre-service mathematics teachers have been met to a very large extent. Recommendations were thereof made accordingly.
Proximate Composition, Physical Properties and Bio-Chemical Traits of Fresh Fenugreek Leafy Vegetables: A Comprehensive Study
Authors: Begum Shourathunnisa , Ramappa K. T. , Nidoni Udaykumar , Hiregoudar Sharanagouda , Ramesh G.
Published: July 2023

The present investigation was undertaken in the Department of Processing and Food Engineering, College of Agricultural Engineering, Raichur to study the different properties of fresh fenugreek leafy vegetable like proximate composition, physical and bio-chemical properties. Fresh fenugreek leaves, also known as methi leaves, are not only a flavourful ingredient but also offer several nutritional benefits. Objective of this study is to determine nutritional compounds of fenugreek leaves and their health benefits. Fresh fenugreek leaves are a rich source of vitamins and minerals. These are essential for maintaining a healthy immune system, promoting good vision, supporting blood clotting, and aiding in the production of new cells. Fenugreek leaves are packed with antioxidants, such as flavonoids (559.81 mg/100g), polyphenols (147.82 mg/100g), and carotenoids (20.25 mg/100g). These compounds help protect the body against damage from harmful free radicals, which can contribute to chronic diseases, including cancer and heart disease. Fenugreek leaves are a good source of dietary fiber. Fiber aids in digestion, helps regulate blood sugar levels, promotes a feeling of fullness, and supports healthy cholesterol levels. These leaves contain high amount of fibers about 2.20% consuming an adequate amount of fiber is important for maintaining a healthy digestive system.
Effect of Potassium and Zinc on Yield Attributes and Economics of Mustard [Brassica juncea (L.) Czern.]
Authors: Komatineni Ajay , Tripathi Suman , Singh Rajesh
Published: July 2023

A field experiment was carried out in the North Eastern Plains of Eastern Uttar Pradesh during the rabi season of 2022 at the agricultural research farm of the Department of Agronomy at Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj with the objective of examining the impact of potassium and zinc on mustard [Brassica juncea (L.) Czern.] growth, yield, and economics using a randomised block design with 10 treatments, of which treatments (T1-T9) T1 – 30 kg K/ha + 5 kg Zn/ha, T2 –  30 kg K/ha + 10 kg Zn/ha, T3 – 30 kg K/ha + 15 kg Zn/ha,  T4 – 40 kg K/ha + 5 kg Zn/ha, T5 – 40 kg K/ha + 10 kg Zn/ha, T6 – 40 kg K/ha + 15 kg Zn/ha, T7 – 50 kg K/ha + 5 kg Zn/ha, T8- 50 kg K/ha + 10 kg Zn/ha, T9 – 50 kg K/ha + 15 kg Zn/ha and T10 – Control with three separate replications of potassium, zinc, and different combinations of nitrogen and phosphorus. The soil in the exploratory plot had a sandy loam texture, a natural pH of 7.2, an EC of 0.26 (dS/m), a low level of organic carbon (0.48%), and available amounts of N (225 kg/ha), P (13.60 kg/ha), and K (215.4 kg/ha). The experimental findings showed that 50 kg K/ha + 15 kg Zn/ha resulted in the maximum plant height (193.45 cm), the highest plant dry weight (35.25 gm),  the number of siliqua/plant (302.20), the number of seeds/siliqua (17.60), the seed yield (2685 kg/ha), and the stover yield (6143 kg/ha). The maximum gross return (136910.00 INR/ha), net return (93070.00 INR/ha), and benefit cost ratio (2.11) were recorded for the treatment 50 kg K/ha + 15 kg Zn/ha, in comparison with other treatment combinations.
Determination of Optimum Reaction Velocity for Glucose Production from Cassava Starch Using Gluco Amylase Sourced from Rice
Authors: Selina Onumadu Kelechi , William Olosunde Adebisi , Orua Antia Okon
Published: July 2023

In an attempt to produce glucose from locally sourced materials to boost glucose syrup production and other applications, the optimum reaction velocity which enzyme becomes saturated with the substrate was investigated. In this study, cassava starch (substrate) and glucoamylase (enzyme) from rice were sourced and prepared. The time course of the enzyme hydrolysis was studied at pH 5 and temperature of 400C as optimum conditions. The hydrolysis was carried out at various substrate concentrations. The time course of the reaction for glucose production was monitored at 10 minutes interval for a period of one hour. The glucose concentration produced was plotted against reaction time to evaluate the optimum glucose amount and its corresponding substrate concentration. The reaction velocity was then computed. Also, the experimental data were fitted into Michaelis-Menten model using Statistical Package for Social Scientists to compare the yield of glucose. Results showed optimum yield of glucose concentration was 0.253 g DE L-1 achieved with 2.5 and 3.0% w/v substrate concentration at 50 and 40 minutes respectively. Further analysis gave the optimum reaction velocity range of 0.00506 to 0.00625 of g DE per litre-min.  This range when compared with the model equation result (0.0052 to 0.0068 g DE litre-min) is reasonably close; hence the results from this study are valid. Technical evaluation to standardize the reaction velocity with respect to substrate concentration which may be referred to as specific reaction velocity gave 0.00205 ±0.00005 g D per litre-min per substrate concentration %w/v.