bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an efficiency ranking system for beef cows and effects on feed intake, ruminal fermentation, NDF turnover, and apparent total tract digestibility
Authors: Delver J.J. , Lardner H.A. , McKinnon J.J. , Ribeiro G.O. , Asai-Coakwell M. , Penner G.B.
Published: August 2023

Beef cows ( n = 100) were ranked for efficiency based on cow rump fat thickness at calving, calving date, and calf weaning weight (% dam BW) over 2 years. The nine most (ME) and least efficient (LE) cows were used to compare feed intake and ruminal fermentation using four 26-day periods with decreasing dietary nutrient density. There were no phenotype × diet interactions for variables of primary interest. Rump fat and calf weaning weight were greater, and the calving date was earlier for ME cows than LE cows ( P ≤ 0.032). The ME cows were lighter ( P < 0.001) but had similar DMI ( P = 0.93) to the LE cows, resulting in greater dry matter intake (DMI) %BW ( P < 0.001). Ruminal contraction amplitude height and area ( P ≤ 0.015) and ruminal digesta weight were greater for LE than ME cows ( P = 0.043). Ruminal ash-free neutral detergent fiber (aNDFom) passage was greater for ME cows than LE cows ( P = 0.047), but the rate of aNDFom degradation did not differ ( P = 0.69). Total tract digestibility did not differ. Efficient cows had greater rump fat, weaned heavier calves, ate more relative to their BW, had a smaller ruminal digesta mass, and had greater ruminal passage of aNDFom without reducing digestibility.
A needle in a haystack: strontium isotopes (87Sr/86Sr) in otoliths identify origin of largemouth bass from a large Southwest reservoir
Authors: Vaisvil Alexander , Willmes Malte , Enriquez Edward J. , Klein Zachary B. , Caldwell Colleen A.
Published: August 2023

Largemouth bass Micropterus salmoides, a popular warm water sport fish, is routinely stocked in reservoirs throughout the USA to augment wild populations. Evaluating if these supplementations are successful requires distinguishing hatchery-sourced fish from their wild counterparts. From 2011 to 2019, over 467 000 largemouth bass fingerlings were stocked from multiple hatchery sources into a large southwestern reservoir (Elephant Butte Reservoir, New Mexico, USA) to supplement the sport fish population. To identify hatchery-sourced largemouth bass, we measured strontium isotope ratios (87Sr/86Sr) and determined ages using otoliths and dorsal spines. Otolith analysis of 169 fish classified 92.9% ( n = 159) to the reservoir with few fish of hatchery origin (2 from Arkansas, 1.2%, and 1 from Montana, 0.6%). While stocking over 467 000 fingerlings across 8 years appears to reflect negligible stocking success, it is likely that low and variable stocking densities (average 3.0, range 0.29–7.77 fish·ha−1) contributed to the low stocking success in Elephant Butte Reservoir. Dorsal fin spines did not yield accurate age reconstructions and their 87Sr/86Sr values were affected by matrix interferences preventing source assignments.
Standard metabolic rate differs between rainbow trout (Oncorhynchus mykiss) growth forms
Authors: Greenaway B. , Veneruzzo C. , Rennie M.D.
Published: August 2023

In variable environments, repeatable phenotypic differences between individuals provide the variation required for natural selection. The pace-of-life syndrome (POLS) provides a conceptual framework linking individual physiology and life histories to behaviour, where rapidly growing individuals demonstrate higher rates of resting or “standard” metabolic rate (SMR). If differences in SMR are consistent between fast- and slow-growing individuals, these differences may be important to capture in bioenergetic relationships used to describe their growth, energy acquisition, and allocation. We compared growth rates and SMR between a domesticated and wild strain of rainbow trout ( Oncorhynchus mykiss (Walbaum, 1792)) using intermittent flow respirometry. Though mass-scaling exponents were similar between strains, mass-scaling coefficients of SMR for fast-growing rainbow trout were 1.25 times higher than those for slower growing fish. These observed differences in mass-scaling coefficients between fast- and slow-growing rainbow trout were consistent with data extracted from several other studies. Bioenergetic estimates of consumption for domestic strain fish increased as the difference in SMR and wild strain fish increased, and increased as activity level increased. Our results indicate patterns of SMR consistent with POLS, and suggest that strain-specific SMR equations may be important for applications to active populations (i.e., field observations).
Analysis of the D-wave $$\Sigma $$-type charmed baryon states with the QCD sum rules
Authors: Wang Zhi-Gang , Lu Fei , Liu Yang
Published: August 2023

We construct the $$\Sigma $$
Σ
-type currents to investigate the D-wave charmed baryon states with the QCD sum rules systematically. The predicted masses $$M=3.35^{+0.13}_{-0.18}\,\textrm{GeV}$$

M
=
3
.

35

-
0.18

+
0.13

GeV

($$3.33^{+0.13}_{-0.16}\,\textrm{GeV}$$

3
.

33

-
0.16

+
0.13

GeV

), $$3.34^{+0.14}_{-0.18}\,\textrm{GeV}$$

3
.

34

-
0.18

+
0.14

GeV

($$3.35^{+0.13}_{-0.16}\,\textrm{GeV}$$

3
.

35

-
0.16

+
0.13

GeV

) and $$3.35^{+0.12}_{-0.13}\, \textrm{GeV}$$

3
.

35

-
0.13

+
0.12

GeV

($$3.35^{+0.12}_{-0.14}\,\textrm{GeV}$$

3
.

35

-
0.14

+
0.12

GeV

) for the $$\Omega _c(0,2,{\frac{1}{2}}^+)$$

Ω
c

(
0
,
2
,

1
2

+

)

, $$\Omega _c(0,2,{\frac{3}{2}}^+)$$

Ω
c

(
0
,
2
,

3
2

+

)

and $$\Omega _c(0,2,{\frac{5}{2}}^+)$$

Ω
c

(
0
,
2
,

5
2

+

)

states are in excellent agreement with the experimental data $$ 3327.1\pm 1.2 \text{ MeV }$$

3327.1
±
1.2

MeV

from the LHCb collaboration, and support assigning the $$\Omega _c(3327)$$

Ω
c

(
3327
)

to be the $$\Sigma $$
Σ
-type D-wave $$\Omega _c$$

Ω
c

state with the spin-parity $$J^P={\frac{1}{2}}^+$$

J
P

=

1
2

+

, $${\frac{3}{2}}^+$$

3
2

+

or $${\frac{5}{2}}^+$$

5
2

+

.
The shapes of the knots corresponding to the special Hopfions
Authors: Shi Xuguang
Published: August 2023

Torus knots can be constructed using the Faddeev-Skyrme model. These knots are called Hopfions, whose topology is described by the Hopf charge $$C=W_{1} W_{2} $$

C
=

W
1

W
2

. A string is entangled to form the knot, which is characterized by the linking number Lk, which is the sum of the twisting number Tw and writhing number Wr. In this paper, we investigate the relationships between the knot shapes and Hopfions with different values of $$(W_{1},W_{2} )$$

(

W
1

,

W
2

)

. We find the knots shapes are not equivalent to the Hopfions shapes even if they have same topological charge. For Hopfions with the value of $$(W_{1},W_{2} )$$

(

W
1

,

W
2

)

, the shapes of the knots change with Euler angle $$\theta $$
θ
. The knots have more writhing structure when $$\theta $$
θ
is smaller. If $$W_{1} <W_{2} $$

W
1

<

W
2

the writhing number cannot totally convert to the twisting number. If $$W_{1} >W_{2} $$

W
1

>

W
2

the writhing number can totally convert to the twisting number.
Laplacian-based Cluster-Contractive t-SNE for High-Dimensional Data Visualization
Authors: Sun Yan , Han Yi , Fan Jicong
Published: August 2023

Dimensionality reduction techniques aim at representing high-dimensional data in low-dimensional spaces to extract hidden and useful information or facilitate visual understanding and interpretation of the data. However, few of them take into consideration the potential cluster information contained implicitly in the high-dimensional data. In this article, we propose
Lap
tSNE, a new graph-layout nonlinear dimensionality reduction method based on t-SNE, one of the best techniques for visualizing high-dimensional data as 2D scatter plots. Specifically,
Lap
tSNE leverages the eigenvalue information of the graph Laplacian to shrink the potential clusters in the low-dimensional embedding when learning to preserve the local and global structure from high-dimensional space to low-dimensional space. It is nontrivial to solve the proposed model because the eigenvalues of normalized symmetric Laplacian are functions of the decision variable. We provide a majorization-minimization algorithm with convergence guarantee to solve the optimization problem of
Lap
tSNE and show how to calculate the gradient analytically, which may be of broad interest when considering optimization with Laplacian-composited objective. We evaluate our method by a formal comparison with state-of-the-art methods on seven benchmark datasets, both visually and via established quantitative measurements. The results demonstrate the superiority of our method over baselines such as t-SNE and UMAP. We also provide out-of-sample extension, large-scale extension, and mini-batch extension for our
Lap
tSNE to facilitate dimensionality reduction in various scenarios.
circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis
Authors: Yan Manli , Hu Caihong , Hu Qi , Ma Heran , Lei Changjiang , Liu Yamei
Published: August 2023

Background. Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. Methods. The detection of circ_0008285 level in glioma specimens and cell lines was conducted by quantitative real-time polymerase chain reaction. The chi-squared test was employed to evaluate the relationship between the circ_0008285 level and the clinical features of glioma patients. The roles of circ_0008285 on the proliferation and apoptosis of glioma cells were studied by knockdown experiment. Meanwhile, the regulatory relationship of circ_0008285, miR-384, and high mobility group protein B1 (HMGB1) was explored in glioma cells, and we explored the effects of circ_0008285/miR-384/HMGB1 pathway on glioma cells. Results. In glioma specimens and cell lines, the expression of circ_0008285 was significantly increased, and a high circ_0008285 level was associated with a larger tumor size and more advanced grading in glioma patients. Furthermore, downregulating circ_0008285 suppressed proliferation and triggered apoptosis of glioma cells, which was associated with a cell cycle arrest at the G1/G0 phase. Mechanism studies indicated that circ_0008285 regulated HMGB1 by sponging miR-384. Functional experiments demonstrated that circ_0008285 promoted the malignant phenotype of glioma cells by miR-384/HMGB1 axis. Conclusion. Our study revealed circ_0008285 as a novel oncogenic factor in glioma through modulating the miR-384/HMGB1 pathway, suggesting that targeting circ_0008285 could serve as a strategy for glioma management.
A Comparative Study on the Dynamic Tensile Strength Evaluation Method of Rock Materials
Authors: Oh Se-Wook , Choi Byung-Hee , Jung Yong-Bok , Cho Sang-Ho
Published: August 2023

The representative calculation methods of dynamic tensile strength applied to the spalling test are comparatively reviewed in the same experimental cases. The results are compared with those of the ISRM suggested dynamic BD method. Through comparative verification results and analysis of methodological characteristics of each determination method in the spalling test, the most reasonable method for calculating dynamic tensile strength in the high strain rate condition is derived. The local strain rate and apparent strain rate are calculated for the same experimental cases, and the difference in the result values according to the strain rate calculation technique is analyzed through comparative verification of the calculated results. Consequently, the characteristics of each dynamic tensile strength determination method and strain rate calculation method in the spalling test are presented, and the results of the review on the most suitable method for calculating the dynamic tensile strength of rock materials are presented.
Sharing the Burden: Latinx Immigrant Parents and Teens’ Sociopolitical Discussions and Their Impact on Youth Mental Health
Authors: Blanco Martinez Saraí , Aramburu Jasmin , Lucio Joel , Grimes Emma , Cross Fernanda Lima
Published: August 2023

Background. There is limited research on parent-child discussions about sociopolitical issues in the US and how they take place. There is less known about the role of sociopolitical conversations as a protective factor benefitting immigrant youth and families. We draw on the ecological expansion of the adverse childhood experience framework to better understand how immigrant-origin youth are making sense of restrictive immigration policies coupled with cultural and sociopolitical messaging received from parents. Methods. Participants engaged in one-hour virtual interviews between 2020 and 2021. We conducted ten interviews with undocumented Latinx parents and 10 interviews with their adolescents aged 13-17. Results. Three main themes emerged from parent interviews: (1) sociopolitical socialization and youth agency, (2) documentation status socialization, and (3) emotional and mental health well-being. Findings show that parents use storytelling to share messages about race, culture, and immigration and provide counternarratives to the toxic sociopolitical environment. Four themes emerged from youth interviews: (1) sociopolitical awareness and action; (2) youth taking on a protective role; (3) learning about risks, injustices, and privileges; and (4) mental health. Youth shared a desire for sociopolitical education and reported a range of coping mechanisms against anti-immigrant rhetoric. Conclusion and Implication. Our findings provide a greater understanding of communication practices within Latinx mixed-status immigrant families, by drawing on both parent and youth reports. These findings can inform practitioners and researchers alike of the amplified systemic barriers felt by immigrant families during the pandemic and the urgency of supporting them as they fight for their rights and dignity.
Time-Dependent Reliability Analysis of Fracture Failure of Corroded Cast Iron Water Pipes and Bayesian Updating for Lifetime Prediction
Authors: Ji Jian , Xie Xiaolei , Fu Guoyang , Kodikara Jayantha
Published: August 2023

Affected by the underground soil environment, buried cast iron pipelines are subject to corrosion during their long-term service, resulting in damage accumulation to the pipe wall and the eventual fracture failure of pipes. This paper aims to propose a probabilistic method to quantitively assess the time-dependent reliability of fracture failure of corroded cast iron pipes. A Gamma-based corrosion process of the pipe wall is derived according to the reported corrosion models. The first-order reliability method and the Monte Carlo simulation are used to cross-validate and conduct the time-dependent reliability analysis based on the fracture failure criterion. Furthermore, uncertain physical parameters of the pipes are updated by the Bayesian Markov Chain Monte Carlo (MCMC) algorithm based on the regional historical data of failed pipes, and lifetime predictions of buried cast iron pipelines are then obtained. A worked example is provided to illustrate the application of the proposed method. It is found that the Gamma process can well simulate the corrosion process hence can be employed to calculate the probability of pipe fracture failure. Sensitivity analysis reveals that the pipe internal water pressure, the fracture toughness, and the geometry of corrosion pits are the most influential parameters to the probability of fracture failure. It is also found that the predicted lifetime of corroded cast iron pipes in the worked example decreases from 110 to 85 years after the Bayesian updating.
BamnetTL: Bidirectional Attention Memory Network with Transfer Learning for Question Answering Matching
Authors: Su Lei , Guo Jiazhi , Wu Liping , Deng Han
Published: August 2023

In KBQA (knowledge base question answering), questions are processed using NLP (natural language processing), and knowledge base technology is used to generate the corresponding answers. KBQA is one of the most challenging tasks in the field of NLP. Q&A (question and answer) matching is an important part of knowledge base QA (question answering), in which the correct answer is selected from candidate answers. At present, Q&A matching task faces the problem of lacking training data in new fields, which leads to poor performance and low efficiency of the question answering system. The paper puts forward a KBQA Q&A matching model for deep feature transfer based on a bidirectional attention memory network, BamnetTL. It uses biattention to collect information from the knowledge base and question sentences in both directions in order to improve the accuracy of Q&A matching and transfers knowledge from different fields through a deep dynamic adaptation network. BamnetTL improves the accuracy of Q&A matching in the target domain by transferring the knowledge in the source domain with more training resources to the target domain with fewer training resources. The experimental results show that the proposed method is effective.
Anterior Palatal Fistula Formation after Le Fort I Osteotomy in Conventional Orthognathic Surgery
Authors: Nezafati Saeed , Pourlak Tannaz
Published: August 2023

The prevalence rate of maxillary ischemic complications following Le Fort I osteotomy was estimated to be about 1%. Understanding the local and systemic factors affecting maxillary perfusion before, during, and after the surgery can prevent the development of these complications. The present study investigated a case of anterior palatal fistula following the classic Le Fort I osteotomy.
Evaluation of the Shear Bond Strength of Chitosan Nanoparticles-Containing Orthodontic Primer: An In Vitro Study
Authors: Mohammed Rawnaq R. , Rafeeq Reem A.
Published: August 2023

Objectives. The present study was intended to investigate the effect of different concentrations of chitosan nanoparticles mixed with an orthodontic primer on the shear bond strength and bond failure of stainless steel brackets bonded to dental enamel. Methods. Four concentrations of chitosan nanoparticles (0%, 1%, 5%, and 10%) were prepared and mixed with Transbond™ XT primer. Forty-eight extracted maxillary first premolars were bonded under a standardized procedure with stainless steel orthodontic brackets utilizing those different concentrations (12 teeth per each group). After the bonding procedure, the specimens were stored in deionized water (37°C for 24 hr) and then thermocycling 5,000 times before shear bond testing, which was performed using a universal testing device. Bond failure sites were examined under a stereomicroscope. Scanning electron microscopy and X-ray diffraction were also performed to verify and evaluate the phase of the nanopowder. Results. The data were statistically analyzed using one-way analysis of variance) and Kruskal–Wallis H tests, and the findings revealed statistically nonsignificant group differences regarding the shear bond strength and adhesive remnant index (

p
>
0.05

). Conclusions. Primers containing varying concentrations of chitosan nanoparticles demonstrated acceptable shear bonding strength and adhesive remnant index.
Data from Small Molecule Inhibitors of TEAD Auto-palmitoylation Selectively Inhibit Proliferation and Tumor Growth of NF2-deficient Mesothelioma
Authors: Tang Tracy T. , Konradi Andrei W. , Feng Ying , Peng Xiao , Ma Mingyue , Li Jian , Yu Fa-Xing , Guan Kun-Liang , Post Leonard
Published: August 2023

Abstract
Mutations in the neurofibromatosis type 2 (NF2) gene that limit or abrogate expression of functional Merlin are common in malignant mesothelioma. Merlin activates the Hippo pathway to suppress nuclear translocation of YAP and TAZ, the major effectors of the pathway that associate with the TEAD transcription factors in the nucleus and promote expression of genes involved in cell proliferation and survival. In this article, we describe the discovery of compounds that selectively inhibit YAP/TAZ-TEAD promoted gene transcription, block TEAD auto-palmitoylation, and disrupt interaction between YAP/TAZ and TEAD. Optimization led to potent analogs with excellent oral bioavailability and pharmacokinetics that selectively inhibit NF2-deficient mesothelioma cell proliferation in vitro and growth of subcutaneous tumor xenografts in vivo. These highly potent and selective TEAD inhibitors provide a way to target the Hippo-YAP pathway, which thus far has been undruggable and is dysregulated frequently in malignant mesothelioma and in other YAP-driven cancers and diseases.
Data from A targeted thorium-227 conjugate demonstrates efficacy in preclinical models of acquired drug resistance and combination potential with chemotherapeutics and antiangiogenic therapies
Authors: Zitzmann-Kolbe Sabine , Kristian Alexander , Zopf Dieter , Kamfenkel Claudia , Politz Oliver , Ellingsen Christine , Hilbig Jochen , Juul Mark U. , Fonslet Jesper , Nielsen Carsten H. , Schatz Christoph A. , Bjerke Roger M. , Cuthbertson Alan S. , Mumberg Dominik , Hagemann Urs B.
Published: August 2023

Abstract
Targeted alpha therapies (TATs) are an innovative class of for cancer treatment. The unique mode-of-action of TATs is the induction of deleterious DNA double-strand breaks. Difficult-to-treat cancers, such as gynecological cancers upregulating the chemoresistance P-glycoprotein (p-gp) and overexpressing the membrane protein mesothelin (MSLN), are promising targets for TATs. Here, based on the previous encouraging findings with monotherapy, we investigated the efficacy of the mesothelin-targeted thorium-227 conjugate (MSLN-TTC) both as monotherapy and in combination with chemotherapies and antiangiogenic compounds in ovarian (OvCa) and cervical cancer models expressing p-gp.
MSLN-TTC monotherapy showed equal cytotoxicity in vitro in p-gp-positive and negative cancer cells, while chemotherapeutics dramatically lost activity on p-gp-positive cancer cells. In vivo, MSLN-TTC exhibited dose-dependent tumor growth inhibition with T/C ratios of 0.03–0.44 in various xenograft models irrespective of p-gp expression status. Furthermore, MSLN-TTC was more efficacious in p-gp-expressing tumors than chemotherapeutics. In the MSLN-expressing ST206B OvCa PDX model, MSLN-TTC accumulated specifically in the tumor, which combined with pegylated liposomal doxorubicin (doxil), docetaxel, bevacizumab, or regorafenib treatment induced additive-to-synergistic antitumor efficacy and substantially increased response rates compared with respective monotherapies. The combination treatments were well tolerated and only transient decreases in white and red blood cells were observed.
In summary, we demonstrate that MSLN-TTC treatment shows efficacy in p-gp-expressing models of chemoresistance and has combination potential with chemo- and antiangiogenic therapies.
Data from A Targeted Thorium-227 Conjugate Demonstrates Efficacy in Preclinical Models of Acquired Drug Resistance and Combination Potential with Chemotherapeutics and Antiangiogenic Therapies
Authors: Zitzmann-Kolbe Sabine , Kristian Alexander , Zopf Dieter , Kamfenkel Claudia , Politz Oliver , Ellingsen Christine , Hilbig Jochen , Juul Mark U. , Fonslet Jesper , Nielsen Carsten H. , Schatz Christoph A. , Bjerke Roger M. , Cuthbertson Alan S. , Mumberg Dominik , Hagemann Urs B.
Published: August 2023

Abstract
Targeted alpha therapies (TAT) are an innovative class of therapies for cancer treatment. The unique mode-of-action of TATs is the induction of deleterious DNA double-strand breaks. Difficult-to-treat cancers, such as gynecologic cancers upregulating the chemoresistance P-glycoprotein (p-gp) and overexpressing the membrane protein mesothelin (MSLN), are promising targets for TATs. Here, based on the previous encouraging findings with monotherapy, we investigated the efficacy of the mesothelin-targeted thorium-227 conjugate (MSLN-TTC) both as monotherapy and in combination with chemotherapies and antiangiogenic compounds in ovarian and cervical cancer models expressing p-gp. MSLN-TTC monotherapy showed equal cytotoxicity in vitro in p-gp–positive and -negative cancer cells, while chemotherapeutics dramatically lost activity on p-gp–positive cancer cells. In vivo, MSLN-TTC exhibited dose-dependent tumor growth inhibition with treatment/control ratios of 0.03–0.44 in various xenograft models irrespective of p-gp expression status. Furthermore, MSLN-TTC was more efficacious in p-gp–expressing tumors than chemotherapeutics. In the MSLN-expressing ST206B ovarian cancer patient-derived xenograft model, MSLN-TTC accumulated specifically in the tumor, which combined with pegylated liposomal doxorubicin (Doxil), docetaxel, bevacizumab, or regorafenib treatment induced additive-to-synergistic antitumor efficacy and substantially increased response rates compared with respective monotherapies. The combination treatments were well tolerated and only transient decreases in white and red blood cells were observed. In summary, we demonstrate that MSLN-TTC treatment shows efficacy in p-gp–expressing models of chemoresistance and has combination potential with chemo- and antiangiogenic therapies.
Supplementary Table 1 from Granulocyte–Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint Blockade
Authors: Li Xiaoyu , Li Jingjing , Zheng Yue , Lee Sandra J. , Zhou Jun , Giobbie-Hurder Anita , Butterfield Lisa H. , Dranoff Glenn , Hodi F. Stephen
Published: August 2023

Supplementary table 1
Supplementary Figure Legends from Granulocyte–Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint Blockade
Authors: Li Xiaoyu , Li Jingjing , Zheng Yue , Lee Sandra J. , Zhou Jun , Giobbie-Hurder Anita , Butterfield Lisa H. , Dranoff Glenn , Hodi F. Stephen
Published: August 2023

Supplementary figure legends
Supplementary Figure 7 from Granulocyte–Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint Blockade
Authors: Li Xiaoyu , Li Jingjing , Zheng Yue , Lee Sandra J. , Zhou Jun , Giobbie-Hurder Anita , Butterfield Lisa H. , Dranoff Glenn , Hodi F. Stephen
Published: August 2023

Supplementary Figure 7
Supplementary Figure 4 from Granulocyte–Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint Blockade
Authors: Li Xiaoyu , Li Jingjing , Zheng Yue , Lee Sandra J. , Zhou Jun , Giobbie-Hurder Anita , Butterfield Lisa H. , Dranoff Glenn , Hodi F. Stephen
Published: August 2023

Supplementary figure 4
Supplementary Table 2 from Systemic Alterations in Type-2 Conventional Dendritic Cells Lead to Impaired Tumor Immunity in Pancreatic Cancer
Authors: James C. Alston , Baer John M. , Zou Chong , Panni Usman Y. , Knolhoff Brett L. , Hogg Graham D. , Kingston Natalie L. , Kang Liang-I. , Lander Varintra E. , Luo Jingqin , Tao Yu , Watson Mark A. , Aft Rebecca , Fields Ryan C. , Hawkins William G. , DeNardo David G.
Published: August 2023

Supplementary Table 2
Supplementary Table S4 from Immunogenetic Metabolomics Reveals Key Enzymes That Modulate CAR T-cell Metabolism and Function
Authors: Renauer Paul , Park Jonathan J. , Bai Meizhu , Acosta Arianny , Lee Won-Ho , Lin Guang Han , Zhang Yueqi , Dai Xiaoyun , Wang Guangchuan , Errami Youssef , Wu Terence , Clark Paul , Ye Lupeng , Yang Quanjun , Chen Sidi
Published: August 2023

Supplementary Table S4
Supplementary Tables from Small Gene Networks Delineate Immune Cell States and Characterize Immunotherapy Response in Melanoma
Authors: Egan Donagh , Kreileder Martina , Nabhan Myriam , Iglesias-Martinez Luis F. , Dovedi Simon J. , Valge-Archer Viia , Grover Amit , Wilkinson Robert W. , Slidel Timothy , Bendtsen Claus , Barrett Ian P. , Brennan Donal J. , Kolch Walter , Zhernovkov Vadim
Published: August 2023

Supplementary Tables S1 and S2
Supplementary Table S3 from Immunogenetic Metabolomics Reveals Key Enzymes That Modulate CAR T-cell Metabolism and Function
Authors: Renauer Paul , Park Jonathan J. , Bai Meizhu , Acosta Arianny , Lee Won-Ho , Lin Guang Han , Zhang Yueqi , Dai Xiaoyun , Wang Guangchuan , Errami Youssef , Wu Terence , Clark Paul , Ye Lupeng , Yang Quanjun , Chen Sidi
Published: August 2023

Supplementary Table S3
Supplemental Table 1 from Synthesizing a Smarter CAR T Cell: Advanced Engineering of T-cell Immunotherapies
Authors: Zhu Iowis , Piraner Dan I. , Roybal Kole T.
Published: August 2023

Summary of chimeric immune receptors designed to incorporate multiple intracellular signaling domains distributed among discrete polypeptide chains.
Supplementary Figures from Immunogenetic Metabolomics Reveals Key Enzymes That Modulate CAR T-cell Metabolism and Function
Authors: Renauer Paul , Park Jonathan J. , Bai Meizhu , Acosta Arianny , Lee Won-Ho , Lin Guang Han , Zhang Yueqi , Dai Xiaoyun , Wang Guangchuan , Errami Youssef , Wu Terence , Clark Paul , Ye Lupeng , Yang Quanjun , Chen Sidi
Published: August 2023

Supplementary Figures 1-10
Table S2 from Circulating and Intratumoral Immune Determinants of Response to Atezolizumab plus Bevacizumab in Patients with Variant Histology or Sarcomatoid Renal Cell Carcinoma
Authors: Saliby Renee Maria , El Zarif Talal , Bakouny Ziad , Shah Valisha , Xie Wanling , Flippot Ronan , Denize Thomas , Kane M. Harry , Madsen Katrine N. , Ficial Miriam , Hirsch Laure , Wei Xiao X. , Steinharter John A. , Harshman Lauren C. , Vaishampayan Ulka N. , Severgnini Mariano , McDermott David F. , Lee Gwo-Shu Mary , Xu Wenxin , Van Allen Eliezer M. , McGregor Bradley A. , Signoretti Sabina , Choueiri Toni K. , McKay Rana R. , Braun David A.
Published: August 2023

Supplementary table 2
Table S1 from Circulating and Intratumoral Immune Determinants of Response to Atezolizumab plus Bevacizumab in Patients with Variant Histology or Sarcomatoid Renal Cell Carcinoma
Authors: Saliby Renee Maria , El Zarif Talal , Bakouny Ziad , Shah Valisha , Xie Wanling , Flippot Ronan , Denize Thomas , Kane M. Harry , Madsen Katrine N. , Ficial Miriam , Hirsch Laure , Wei Xiao X. , Steinharter John A. , Harshman Lauren C. , Vaishampayan Ulka N. , Severgnini Mariano , McDermott David F. , Lee Gwo-Shu Mary , Xu Wenxin , Van Allen Eliezer M. , McGregor Bradley A. , Signoretti Sabina , Choueiri Toni K. , McKay Rana R. , Braun David A.
Published: August 2023

Supplementary table 1
Figure S5 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer
Authors: Suzuki Toshiyasu , Kilbey Anna , Casa-Rodríguez Nuria , Lawlor Amy , Georgakopoulou Anastasia , Hayman Hannah , Yin Swe Kyi Lai , Nordin Anna , Cantù Claudio , Vantourout Pierre , Ridgway Rachel A. , Byrne Ryan M. , Chen Lei , Verzi Michael P. , Gay David M. , Gil Vázquez Ester , Belnoue-Davis Hayley L. , Gilroy Kathryn , Køstner Anne Helene , Kersten Christian , Thuwajit Chanitra , Andersen Ditte K. , Wiesheu Robert , Jandke Anett , Blyth Karen , Roseweir Antonia K. , Leedham Simon J. , Dunne Philip D. , Edwards Joanne , Hayday Adrian , Sansom Owen J. , Coffelt Seth B.
Published: August 2023

Disruption of WNT gradient in normal intestinal villi reduces gamma delta T cells
Figure S5 from Circulating and Intratumoral Immune Determinants of Response to Atezolizumab plus Bevacizumab in Patients with Variant Histology or Sarcomatoid Renal Cell Carcinoma
Authors: Saliby Renee Maria , El Zarif Talal , Bakouny Ziad , Shah Valisha , Xie Wanling , Flippot Ronan , Denize Thomas , Kane M. Harry , Madsen Katrine N. , Ficial Miriam , Hirsch Laure , Wei Xiao X. , Steinharter John A. , Harshman Lauren C. , Vaishampayan Ulka N. , Severgnini Mariano , McDermott David F. , Lee Gwo-Shu Mary , Xu Wenxin , Van Allen Eliezer M. , McGregor Bradley A. , Signoretti Sabina , Choueiri Toni K. , McKay Rana R. , Braun David A.
Published: August 2023

Supplementary Figure 5
Data from Systemic Alterations in Type-2 Conventional Dendritic Cells Lead to Impaired Tumor Immunity in Pancreatic Cancer
Authors: James C. Alston , Baer John M. , Zou Chong , Panni Usman Y. , Knolhoff Brett L. , Hogg Graham D. , Kingston Natalie L. , Kang Liang-I. , Lander Varintra E. , Luo Jingqin , Tao Yu , Watson Mark A. , Aft Rebecca , Fields Ryan C. , Hawkins William G. , DeNardo David G.
Published: August 2023

Abstract
Intratumoral T-cell dysfunction is a hallmark of pancreatic tumors, and efforts to improve dendritic cell (DC)–mediated T-cell activation may be critical in treating these immune therapy unresponsive tumors. Recent evidence indicates that mechanisms that induce dysfunction of type 1 conventional DCs (cDC1) in pancreatic adenocarcinomas (PDAC) are drivers of the lack of responsiveness to checkpoint immunotherapy. However, the impact of PDAC on systemic type 2 cDC2 development and function has not been well studied. Herein, we report the analysis of 3 cohorts, totaling 106 samples, of human blood and bone marrow (BM) from patients with PDAC for changes in cDCs. We found that circulating cDC2s and their progenitors were significantly decreased in the blood of patients with PDAC, and repressed numbers of cDC2s were associated with poor prognosis. Serum cytokine analyses identified IL6 as significantly elevated in patients with PDAC and negatively correlated with cDC numbers. In vitro, IL6 impaired the differentiation of cDC1s and cDC2s from BM progenitors. Single-cell RNA sequencing analysis of human cDC progenitors in the BM and blood of patients with PDAC showed an upregulation of the IL6/STAT3 pathway and a corresponding impairment of antigen processing and presentation. These results suggested that cDC2s were systemically suppressed by inflammatory cytokines, which was linked to impaired antitumor immunity.
Data from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer
Authors: Suzuki Toshiyasu , Kilbey Anna , Casa-Rodríguez Nuria , Lawlor Amy , Georgakopoulou Anastasia , Hayman Hannah , Yin Swe Kyi Lai , Nordin Anna , Cantù Claudio , Vantourout Pierre , Ridgway Rachel A. , Byrne Ryan M. , Chen Lei , Verzi Michael P. , Gay David M. , Gil Vázquez Ester , Belnoue-Davis Hayley L. , Gilroy Kathryn , Køstner Anne Helene , Kersten Christian , Thuwajit Chanitra , Andersen Ditte K. , Wiesheu Robert , Jandke Anett , Blyth Karen , Roseweir Antonia K. , Leedham Simon J. , Dunne Philip D. , Edwards Joanne , Hayday Adrian , Sansom Owen J. , Coffelt Seth B.
Published: August 2023

Abstract
Intraepithelial lymphocytes (IEL) expressing γδ T-cell receptors (γδTCR) play key roles in elimination of colon cancer. However, the precise mechanisms by which progressing cancer cells evade immunosurveillance by these innate T cells are unknown. Here, we investigated how loss of the Apc tumor suppressor in gut tissue could enable nascent cancer cells to escape immunosurveillance by cytotoxic γδIELs. In contrast with healthy intestinal or colonic tissue, we found that γδIELs were largely absent from the microenvironment of both mouse and human tumors, and that butyrophilin-like (BTNL) molecules, which can critically regulate γδIEL through direct γδTCR interactions, were also downregulated in tumors. We then demonstrated that β-catenin activation through loss of Apc rapidly suppressed expression of the mRNA encoding the HNF4A and HNF4G transcription factors, preventing their binding to promoter regions of Btnl genes. Reexpression of BTNL1 and BTNL6 in cancer cells increased γδIEL survival and activation in coculture assays but failed to augment their cancer-killing ability in vitro or their recruitment to orthotopic tumors. However, inhibition of β-catenin signaling via genetic deletion of Bcl9/Bcl9L in either Apc-deficient or mutant β-catenin mouse models restored Hnf4a, Hnf4g, and Btnl gene expression and γδ T-cell infiltration into tumors. These observations highlight an immune-evasion mechanism specific to WNT-driven colon cancer cells that disrupts γδIEL immunosurveillance and furthers cancer progression.
Supplementary Data T5 from A Survey of Cancer Risk Behaviors, Beliefs, and Social Drivers of Health in New Hampshire and Vermont
Authors: Skipper Thomas A , Weiss Julie E , Carlos Heather A , Gunn Christine M , Hasson Rian M , Peacock Janet L , Schiffelbein Jenna E , Tosteson Anna N.A. , Lansigan Frederick , Rees Judy R
Published: August 2023

Social factors by rurality and educational attainment
Supplemental Table S1 from Tusamitamab ravtansine in patients with advanced solid tumors: Phase 1 study of safety, pharmacokinetics, and antitumor activity using alternative dosing regimens
Authors: Tabernero Josep , Bedard Philippe L. , Bang Yung-Jue , Vieito Maria , Ryu Min-Hee , Fagniez Nathalie , Chadjaa Mustapha , Soufflet Christine , Masson Nina , Gazzah Anas
Published: August 2023

Reasons for screen failure
Supplemental Figure S1 from Tusamitamab ravtansine in patients with advanced solid tumors: Phase 1 study of safety, pharmacokinetics, and antitumor activity using alternative dosing regimens
Authors: Tabernero Josep , Bedard Philippe L. , Bang Yung-Jue , Vieito Maria , Ryu Min-Hee , Fagniez Nathalie , Chadjaa Mustapha , Soufflet Christine , Masson Nina , Gazzah Anas
Published: August 2023

Tusamitamab ravtansine maximum plasma concentrations by dose level
Data from Tusamitamab ravtansine in patients with advanced solid tumors: Phase 1 study of safety, pharmacokinetics, and antitumor activity using alternative dosing regimens
Authors: Tabernero Josep , Bedard Philippe L. , Bang Yung-Jue , Vieito Maria , Ryu Min-Hee , Fagniez Nathalie , Chadjaa Mustapha , Soufflet Christine , Masson Nina , Gazzah Anas
Published: August 2023

Abstract
Background:
Tusamitamab ravtansine is an antibody-drug conjugate that targets carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) and delivers a cytotoxic maytansinoid payload. In a Phase 1 dose-escalation study, the maximum tolerated dose (MTD) was 100 mg/m2 every 2 weeks (Q2W). Here we report results for two alternative schedules.
Patients and methods:
Adults aged ≥ 18 years (range 34–73) with locally advanced/metastatic solid tumors (N=43; colon/rectum, 29; stomach, 7; pancreas, 4; other, 3) expressing/likely to express CEACAM5 received intravenous tusamitamab ravtansine 120–170 mg/m2 (loading dose [LD]), then 100 mg/m2 Q2W (Q2W-LD, N=28), or 120–190 mg/m2 fixed dose (Q3W, N=15). The primary endpoint was dose-limiting toxicities (DLTs) during Cycles 1–2 (Q2W-LD) and Cycle 1 (Q3W).
Results:
Reversible DLTs were observed in 2/9 patients (Grade 2 keratopathy; Grade 2 keratitis) with 170 mg/m2 in Q2W-LD and in 2/3 patients (Grade 2 keratopathy; Grade 3 transaminase elevation) with 190 mg/m2 in Q3W. Nineteen (67.9%) patients in Q2W-LD and 13 (86.7%) patients in Q3W experienced treatment-related adverse events (AEs); 3/43 patients discontinued treatment because of AEs. The most common AEs were asthenia, gastrointestinal complaints, keratopathy, keratitis, and peripheral sensory neuropathy. In this small, heavily pretreated population, no confirmed responses were observed; however, stable disease occurred in 35.7% of patients in Q2W-LD and 40.0% of patients in Q3W.
Conclusions:
Tusamitamab ravtansine had a favorable safety profile with both alternative administration schedules; MTDs were 170 mg/m2 (LD) followed by 100 mg/m2 Q2W, and 170 mg/m2 Q3W as a fixed dose. [NCT02187848]
Clinical Prediction Models and Predictors for Death or Adverse Neurodevelopmental Outcome in Term Newborns with Hypoxic-Ischemic Encephalopathy: A Systematic Review of the Literature
Authors: Langeslag Juliette F. , Berendse Kevin , Daams Joost G. , Onland Wes , Leeflang Mariska M.G. , van Kaam Anton H. , de Haan Timo R.
Published: August 2023

Background: Although many predictive parameters have been studied, an internationally accepted, validated predictive model to predict the clinical outcome of asphyxiated infants suffering from hypoxic-ischemic encephalopathy is currently lacking. The aim of this study was to identify, appraise and summarize available clinical prediction models, and provide an overview of all investigated predictors for the outcome death or neurodevelopmental impairment in this population. Methods: A systematic literature search was performed in Medline and Embase. Two reviewers independently included eligible studies and extracted data. The quality was assessed using PROBAST for prediction model studies and QUIPS assessment tools for predictor studies. Results: A total of nine prediction models were included. These models were very heterogeneous in number of predictors assessed, methods of model derivation, and primary outcomes. All studies had a high risk of bias following the PROBAST assessment and low applicability due to complex model presentation. A total of 104 predictor studies were included investigating various predictors, showing tremendous heterogeneity in investigated predictors, timing of predictors, primary outcomes, results, and methodological quality according to QUIPS. Selected high-quality studies with accurate discriminating performance provide clinicians and researchers an evidence map of predictors for prognostication after HIE in newborns. Conclusion: Given the low methodological quality of the currently published clinical prediction models, implementation into clinical practice is not yet possible. Therefore, there is an urgent need to develop a prediction model which complies with the PROBAST guideline. An overview of potential predictors to include in a prediction model is presented.
Adult Henoch-Schönlein Purpura: Comprehensive Assessment of Demographic, Clinical, and Histopathological Features as Predictors for Systemic Involvement
Authors: Hammad Helwe , Krausz Judit , Barcan Moran , Fisher Shani , Edison Natasha , Ziv Michael
Published: August 2023

Background: Henoch-Schönlein purpura (HSP) is a small-vessel IgA-predominant vasculitis. A major challenge in managing adult HSP is the difficulty assessing the risk of systemic involvement. There is currently a paucity of data in this area. Objective: The objective of this study was to determine demographic, clinical, and histopathological features associated with systemic involvement in adult HSP. Methods: In this retrospective study, we reviewed demographical features and clinical and pathology data of 112 adult HSP patients seen at Emek Medical Center between January 2008 and December 2020. Results: Of these patients, 41 (36.6%) had renal involvement, 24 (21.4%) had gastrointestinal tract involvement, and 31 (27.7%) had joint involvement. Age >30 years (p = 0.006) at diagnosis was an independent predictor of renal involvement. Platelet count (<150 K/µL) (p = 0.020) and apoptosis of keratinocytes on skin biopsy (p = 0.031) were also associated with renal involvement. History of autoimmune disease (p = 0.001), positive c-antineutrophil cytoplasmic antibody (p = 0.018), positive rheumatoid factor (p = 0.029), and elevated erythrocyte sedimentation rate (p = 0.04) were associated with joint involvement. Female sex (p = 0.003), Arab race (p = 0.036), and positive pANCA (p = 0.011) were associated with gastrointestinal tract involvement. Limitations: This study is retrospective. Conclusion: These findings may serve as a guide to stratify risk in adult HSP patients so that those at higher risk can be monitored more closely.
ETHNOBOTANICAL STUDY OF MEDICINAL PLANT USAGE DURING COVID-19 PANDEMIC: A COMMUNITY-BASED SURVEY IN INDONESIA
Authors: Nayaka Ni Made Dwi Mara Widyani , Yuda Putu Era Sandhi Kusuma , Sanjaya Dwi Arymbhi , Ernawati Desak Ketut , Cahyaningsih Erna , Dewi Ni Luh Kade Arman Anita , Sasadara Maria Malida Vernandes
Published: August 2023

Before the availability of a vaccine, Indonesian population relied on traditional medicines to prevent COVID-19. Any species used by indigenous people could lead to further investigations in modern pharmacology, to preserve ancient knowledge, and to plan for plants’ conservation. The study aimed to discover and record species, methods of preparation, route of administration, and motivation in using medicinal plants by the Indonesian population during the COVID-19 pandemic. Participants of survey were selected from the people who live in Java and Bali for responding to an online structured questionnaire. Relative Frequency of Citation (RFC) was employed in the quantitative analysis of the collected data. The pharmacological relevance of the five plants with the highest RFC was further reviewed. The results showed that respondents used 59 plants from 28 families. Five species with the highest RFC were Curcuma longa (0.707), Zingiber officinale (0.674), Cymbopogon citratus (0.269), Kaempferia galanga (0.174), and Curcuma zanthorrhiza (0.165). Most plants were prepared by boiling (77.97%) and administered orally as a single ingredient or mixed with other herbals. Respondents believed that the plants were beneficial as immune-booster (71.26%), maintain good health (24.85%) and stamina (12.28%), and prevent viral infection, including COVID-19 (5.39%). The most commonly used plants might be scientifically based to boost immunity. However, their usage against COVID-19 and the medicinal value of herbal mixtures should be further investigated.
USE OF BIOLOGICAL ORGANIC FERTILIZERS AND PESTICIDES TO IMPROVE POTATO CULTIVATION IN SLOPE ANDISOLS
Authors: Tamad  , Soetanto Loekas , Karim Akhmad Rizqul
Published: August 2023

In the 1990s, potato yield in the Andisols of Dieng, Central Java, Indonesia, was approximately 30 t ha–1, but this value decreased rapidly to 12–15 t ha–1 in recent years. This rapid decline could be attributed to the use of unbalanced organic and chemical fertilizers, without the application of conservation techniques. Therefore, this study aimed to sustainably improve the local potato cultivation pattern of farmers on Andisols using biological organic fertilizers and pesticides (BOFP). A randomized block design was used with two factors, namely: 1) 20 t BOFP, 300 kg Urea, 500 kg SP 36, 300 kg KCl, and 200 kg lime ha–1, and 2) comparison with the pattern of farmers, consisting of 20 t of chicken manure, 1–t NPK, and 250 kg ZA ha–1. The potato plant mounds were tilted 10% to the contour direction, and each treatment was carried out with 16 replications. Granola seeds were used to plant potato during the rainy season from March-June 2022. The results showed that the plants cultivated using the local pattern of farmers were affected by wilt from Fusarium spp, while the use of BOFP decreased the incidence of the disease by 80%. Furthermore, the BOFP pattern significantly increased Andisols organic-C from 1.78% to 3.83% and total soil P from 5.20% to 11.34%, compared to the pattern of farmers. It also increased potato yields from 12.31 t ha–1 to 22.93 t ha–1 and the R/C from 0.85 to 1.23, compared to the pattern of farmers. Based on the results, the use of BOFP pattern decreased wilt attacks by Fusarium spp, improved the productivity of Andisols, as well as increased potato production and profits of farmers.