bluuebunny/crossref_metadata_2025 huggingface dataset:
A Prospective Investigation of Predictive Parameters for Preoperative Volume Assessment in Breast Reconstruction
Authors: Zingaretti Nicola , Miotti Giovanni , Maronese Carlo Alberto , Isola Miriam , Guarneri Gianni Franco , Albanese Roberta , De Francesco Francesco , Riccio Michele , Cereser Lorenzo , Zuiani Chiara , Parodi Pier Camillo
Published: November 2021

Preoperative breast volume estimation is very important for the success of the breast surgery. In this study four different breast volume determination methods were compared. The end-point of this prospective study was to evaluate the concordance between different modalities of breast volume assessment (MRI, BREAST-V, mastectomy specimen weight, conversion from weight to volume of mastectomy specimen) and the breast prosthetic volume implanted. The study enrolled 64 patients between 2017 and 2019, who had all been treated by the same surgeons for monolateral nipple–areola complex-sparing mastectomy and implant breast reconstruction. Only patients who had a breast reconstruction classified as “excellent” from an objective (BCCT.core software) and subjective (questionnaire) point of view at the 6-month interval after the operation were included in the study. Data analysis highlighted a strong correlation between the volumes of the chosen prostheses and the weights of mastectomy converted into volume, especially for patients with grades B and C parenchymal density. The values of the agreement between the volumes of the chosen prostheses and the assessments from MRI and BREAST -V proved to be lower than expected from the literature. None of the four studied methods presented any strong correlation with the initial breast width. Our results suggest that conversion from weight to volume of mastectomy specimen should be used to assist in determining the volume of the breast implant to be implanted. This method would help the reconstructive surgeon guide the choice of the most appropriate implant preoperatively.
Bright Side of Fusarium oxysporum: Secondary Metabolites Bioactivities and Industrial Relevance in Biotechnology and Nanotechnology
Authors: Ibrahim Sabrin R. M. , Sirwi Alaa , Eid Basma G. , Mohamed Shaimaa G. A. , Mohamed Gamal A.
Published: November 2021

Fungi have been assured to be one of the wealthiest pools of bio-metabolites with remarkable potential for discovering new drugs. The pathogenic fungi, Fusarium oxysporum affects many valuable trees and crops all over the world, producing wilt. This fungus is a source of different enzymes that have variable industrial and biotechnological applications. Additionally, it is widely employed for the synthesis of different types of metal nanoparticles with various biotechnological, pharmaceutical, industrial, and medicinal applications. Moreover, it possesses a mysterious capacity to produce a wide array of metabolites with a broad spectrum of bioactivities such as alkaloids, jasmonates, anthranilates, cyclic peptides, cyclic depsipeptides, xanthones, quinones, and terpenoids. Therefore, this review will cover the previously reported data on F. oxysporum, especially its metabolites and their bioactivities, as well as industrial relevance in biotechnology and nanotechnology in the period from 1967 to 2021. In this work, 180 metabolites have been listed and 203 references have been cited.
The Effects of Aspergillus fumigatus Colonization on Lung Function in Patients with Cystic Fibrosis
Authors: Al Shakirchi Mahasin , Sorjonen Kimmo , Klingspor Lena , Bergman Peter , Hjelte Lena , de Monestrol Isabelle
Published: November 2021

Aspergillus fumigatus is commonly isolated from CF airways. However, the impact on CF lung progression is not completely understood. In this study, using a 16-year retrospective observational cohort study (2000–2015) that included 132 patients, we determined the annual lung function, measured as percent predicted forced expiratory volume in the first second (ppFEV1), decline before and after the first colonization with A. fumigatus. Further, in the same individual, the ratios of lung function when patients were colonized with A. fumigatus and when they were not were calculated. The impact of eradication, with antifungal treatment or spontaneously, was assessed. The annual ppFEV1 was significantly lower after the first colonization with A. fumigatus. Furthermore, within the same individual, colonization with A. fumigatus for two and three years in a row was associated with 4.3% and 7.9% lower ppFEV1, respectively, compared to when not colonized. Finally, patients who eradicated A. fumigatus the following two years after colonization exhibited 9.9% and 14.5% higher ppFEV1 compared to patients who continued to produce cultures with A. fumigatus for two and three years. Our study demonstrated that A. fumigatus colonization was associated with a negative impact on lung function in the long term and eradication, spontaneously or with treatment, was associated with a better pulmonary outcome.
Quantifying and Characterizing Urban Leisure Activities by Merging Multiple Sensing Big Data: A Case Study of Nanjing, China
Authors: Liu Shaojun , Long Yao , Zhang Ling , Liu Hao
Published: November 2021

Studying the spatiotemporal pattern of urban leisure activities helps us to understand the development and utilization of urban public space, people’s quality of life, and the happiness index. It has outstanding value for improving rational resource allocation, stimulating urban vitality, and promoting sustainable urban development. This study aims at discovering the spatiotemporal distribution patterns and people’s behavioral preferences of urban leisure activities using quantitative technology merging ubiquitous sensing big data. On the basis of modeling individual activity traces using mobile signaling data (MSD), we developed a space-time constrained dasymetric interpolation method to refine the urban leisure activity spatiotemporal distribution. We conducted an empirical study in Nanjing, China. The results indicate that leisure plays an essential role in daily human life, both on weekdays and weekends. Significant differences exist in spatiotemporal and type selection in urban leisure. The weekend afternoon is the breakout period of leisure, and entertainment is the most popular leisure activity. Furthermore, the correlation between leisure resource allocation and leisure activity participation was argued. Our findings confirm that data-driven approaches would be a promising method for analyzing human behavior patterns; therefore, assisting in land planning decisions and promoting social justice and sustainability.
Influence of Nanoscale Inhomogeneity Incorporating Interface Effect on Crack Nucleation at Intersection of Twin and Grain Boundary in Nanocomposite
Authors: Tao Yongshu , Li Liang , Miao Guanghong , Dong Jilei
Published: November 2021

Nanocracks can generate at the intersection of the deformation twin and grain boundary (GB). A mathematical model is built to study the nanoinhomogeneity effect on nanocrack nucleation and propagation in the nanocrystalline matrix. The boundary condition at the interface between the nanoinhomogeneity and the matrix is modified by incorporating the interface effect. The influence of the nanoinhomogeneity shear modulus, the nanoinhomogeneity radius, the nanoinhomogeneity position, the interface effect, and the external stress on the nanocrack nucleation and propagation is investigated in detail. The results indicate that the stiff nanoinhomogeneity suppresses nanocrack nucleation and propagation and thereby improves the tensile ductility of nanocomposites without loss of their predominantly high strength. Both the positive interface residual tension and interface elastic constants suppress nanocrack nucleation and propagation, while the negative interface residual tension and interface elastic constants promote nanocrack nucleation and propagation. Furthermore, the effect of interface residual tension is rather significant. The interface elastic constants have a weak effect on nanocrack nucleation and propagation.
Entrapment of a Cytotoxic Drug into the Crystal Structure of Calcite for Targeted Drug Delivery
Authors: Vazda Amina , Pujari-Palmer Michael , Xia Wei , Engqvist Håkan
Published: November 2021

Controlled drug release and targeted drug delivery can reduce systemic toxicity of chemotherapeutics by restricting drugs to the target organ and increasing the local concentration. As tumors and inflamed tissue are often surrounded by an acidic microenvironment, pH-responsive calcium carbonates (CaCO3) are promising vehicles for controlled drug delivery applications. The aim of this study was to evaluate the loading efficacy and release of a chemotherapeutic drug, Hydroxyurea (HU), into the crystal structure of calcite. Incorporation of HU did not alter the crystallinity, crystal size, or morphology of precipitated calcite crystals, as assessed by XRD and SEM. The amount of HU was quantified by High-Pressure Liquid Chromatography (HPLC) and showed that 6.7 ± 0.7 µg of HU could be for each milligram of calcite (0.016 mol% ± 0.002). In cell media, the optimal pH for controlled release was 5 (0.1 mg/mL released after 1 h). However, in vitro, pH below 6.5 was cytotoxic to human breast cancer cells (MCF-7). Direct contact studies, where particles were incubated with MCF-7 cells, showed that the amount of HU release from calcite was not high enough to kill the cell or arrest growth at pH 6.5. Pre-dissolved release studies, where the particles were pre-dissolved in acidic media to simulate complete drug release in vivo, pH neutralized, and exposed to the cells, showed that the amount of loaded HU reduced the survival/proliferation of MCF7. In conclusion, it is possible to integrate HU into the crystal structure of a calcite crystal and release the drug in vitro at concentrations that can slow the growth of cancer cells, without affecting calcite morphology and crystallinity. Further research is needed to investigate the in vivo behavior of the particles and whether the actual tumor pH is low enough to achieve complete drug release in vivo.
The Domain of Residual Lifetime Attraction for the Classical Distributions of the Reliability Theory
Authors: Rusev Vladimir , Skorikov Alexander
Published: November 2021

The asymptotic behavior of the residual lifetime of the system and its characteristics are studied for the main distributions of reliability theory. Sufficiently precise and simple conditions for the domain of attraction of the exponential distribution are proposed, which are applicable for a wide class of distributions. This approach allows us to take into account important information about modeling the failure-free operation of equipment that has worked reliably for a long time. An analysis of the domain of attraction for popular distributions with “heavy tails” is given.
Optimized Unidirectional and Bidirectional Stiffened Objects for Minimum Material Consumption of 3D Printing
Authors: Zheng Anzong , Zhu Zaiping , Bian Shaojun , Chang Jian , Haron Habibollah , Iglesias Andres , You Lihua , Zhang Jianjun
Published: November 2021

3D printing, regarded as the most popular additive manufacturing technology, is finding many applications in various industrial sectors. Along with the increasing number of its industrial applications, reducing its material consumption and increasing the strength of 3D printed objects have become an important topic. In this paper, we introduce unidirectionally and bidirectionally stiffened structures into 3D printing to increase the strength and stiffness of 3D printed objects and reduce their material consumption. To maximize the advantages of such stiffened structures, we investigated finite element analysis, especially for general cases of stiffeners in arbitrary positions and directions, and performed optimization design to minimize the total volume of stiffened structures. Many examples are presented to demonstrate the effectiveness of the proposed finite element analysis and optimization design as well as significant reductions in the material costs and stresses in 3D printed objects stiffened with unidirectional and bidirectional stiffeners.
Post-Coronavirus Disease-2019 (COVID-19): Toward a Severe Multi-Level Health Crisis?
Authors: Ghanemi Abdelaziz , Yoshioka Mayumi , St-Amand Jonny
Published: November 2021

There were already numerous challenges facing the healthcare system prior to the ongoing coronavirus disease-2019 (COVID-19) pandemic. Although we look forward to ending this pandemic, it is still expected that the healthcare system will face further challenges leading to a multi-level health crisis. Indeed, after the COVID-19 pandemic, there will still be COVID-19 active cases and those left with health problems following COVID-19 infection who will be of a particular impact. In addition, we also have the health problems that either emerged or worsened during COVID-19, especially with the reduced ability of the healthcare system to take care of many non COVID-19 patients during the COVID-19 pandemic. Such expected evolution of the situation highlights the necessity for the decision-makers to consider applying serious reforms and take quick measures to prevent a post-COVID-19 health crisis.
Influence of the Chromium Content in Low-Alloyed Cu–Cr Alloys on the Structural Changes, Phase Transformations and Properties in Equal-Channel Angular Pressing
Authors: Aksenov Denis A. , Asfandiyarov Rashid N. , Raab Georgy I. , Fakhretdinova Elvira I. , Shishkunova Maria A.
Published: November 2021

The quantitative concentration of alloying elements in low-alloyed copper alloys is an important factor in forming electrical and mechanical characteristics. It is known that severe plastic deformation is accompanied by both a substantial refinement of the structure and changes in the kinetics of phase transformations during the deformation and the post-deformation thermal treatment. This paper presents the results of a comparative analysis of the Cu–0.2Cr and Cu–1.1Cr alloys subjected to equal-channel angular pressing at room temperature. The analysis was performed for the grain structure, solid solution, and second-phase particles using transmission electron microscopy, scanning electron microscopy, X-ray crystal analysis, and the small-angle diffraction method. It was found that the level of structure refinement and mechanical characteristics after equal-channel angular pressing was almost the same for both studied alloys. Post-deformation aging of the Cu–0.2Cr alloy leads to the development of polygonization and re-crystallization within it. The aging of the Cu–1.1Cr alloy shows a better thermal stability than that of the Cu–0.2Cr alloy. In the Cu–1.1Cr alloy, after aging, in comparison with Cu–0.2Cr, a denser-packed ensemble of fine particles with an average size of 54 ± 2 nm is formed. In this case, the average size of fragments is 270 ± 15 nm and the ultimate tensile strength reaches 485 MPa.
Ultrasonic Welding of Nickel with Coarse and Ultrafine Grained Structures
Authors: Shayakhmetova Elvina , Murzinova Mariya , Nazarov Ayrat
Published: November 2021

Solid state joints of samples of coarse-grained (CG) and ultrafine-grained (UFG) nickel have been obtained for the first time using spot ultrasonic welding (USW). The UFG structure in disk-shaped samples was processed by means of high-pressure torsion (HPT). On the basis of lap shear tests, the optimal values of the clamping force resulting in the highest values of the joint strength are determined. The microstructures in the weld joints obtained at optimal parameters of USW are characterized by scanning electron microscopy. It is shown that during ultrasonic welding of coarse-grained nickel, a thin layer with an UFG microstructure is formed near the weld surfaces. The bulks of sheets retain the CG microstructure, but a significant dislocation activity is observed in these regions. During USW of samples having an UFG initial microstructure, significant grain growth occurs. Fine grains are observed only along the welding interface. An average lap shear strength of 97 MPa was obtained by welding the UFG samples, which was approximately 40% higher than the strength of samples processed by welding coarse-grained sheets (70 MPa). It is concluded that higher strength weld joints can be obtained by using sheets with the UFG structure as compared to the CG sheets.
Prediction of Mining Conditions in Geotechnically Complex Sites
Authors: Elmouttie Marc , Hodgkinson Jane , Dean Peter
Published: November 2021

Geotechnical complexity in mining often leads to geotechnical uncertainty which impacts both safety and productivity. However, as mining progresses, particularly for strip mining operations, a body of knowledge is acquired which reduces this uncertainty and can potentially be used by mining engineers to improve the prediction of future mining conditions. In this paper, we describe a new method to support this approach based on modelling and neural networks. A high-level causal model of the mining operations based on historical data for a number of parameters was constructed which accounted for parameter interactions, including hydrogeological conditions, weather, and prior operations. An artificial neural network was then trained on this historical data, including production data. The network can then be used to predict future production based on presently observed mining conditions as mining proceeds and compared with the model predictions. Agreement with the predictions indicates confidence that the neural network predictions are properly supported by the newly available data. The efficacy of this approach is demonstrated using semi-synthetic data based on an actual mine.
Characterization of the Trans-Epithelial Transport of Green Tea (C. sinensis) Catechin Extracts with In Vitro Inhibitory Effect against the SARS-CoV-2 Papain-like Protease Activity
Authors: Montone Carmela Maria , Aita Sara Elsa , Arnoldi Anna , Capriotti Anna Laura , Cavaliere Chiara , Cerrato Andrea , Lammi Carmen , Piovesana Susy , Ranaldi Giulia , Laganà Aldo
Published: November 2021

This work describes an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papain-like protease (PLpro) activity. After specific catechin extraction, a chromatographic separation obtained six fractions were carried out. The fractions were assessed in vitro against the PLpro target. Fraction 5 showed the highest inhibitory activity against the SARS-CoV-2 PLpro (IC50 of 0.125 μg mL−1). The untargeted characterization revealed that (−)-epicatechin-3-gallate (ECG) was the most abundant compound in the fraction and the primary molecule absorbed by differentiated Caco-2 cells. Results indicated that fraction 5 was approximately 10 times more active than ECG (IC50 value equal to 11.62 ± 0.47 μg mL−1) to inhibit the PLpro target. Overall, our findings highlight the synergistic effects of the various components of the crude extract compared to isolated ECG.
Organocatalytic Asymmetric [2 + 4] Cycloadditions of 3-Vinylindoles with ortho-Quinone Methides
Authors: Liu Si-Jia , Tu Man-Su , Liu Kai-Yue , Chen Jia-Yi , Ni Shao-Fei , Zhang Yu-Chen , Shi Feng
Published: November 2021

Catalytic asymmetric [2 + 4] cycloadditions of 3-vinylindoles with ortho-quinone methides and their precursors were carried out in the presence of chiral phosphoric acid to afford a series of indole-containing chroman derivatives with structural diversity in overall high yields (up to 98%), good diastereoselectivities (up to 93:7 dr) and moderate to excellent enantioselectivities (up to 98% ee). This approach not only enriches the chemistry of catalytic asymmetric cycloadditions involving 3-vinylindoles but is also useful for synthesizing chiral chroman derivatives.
Determination of Metallic Impurities by ICP-MS Technique in Eyeshadows Purchased in Poland. Part I
Authors: Pawlaczyk Aleksandra , Gajek Magdalena , Balcerek Martyna , Szynkowska-Jóźwik Małgorzata I.
Published: November 2021

Eye shadows, which are products willingly and frequently used by women and even children, have been reported in literature to contain toxic metals. In this work, a total of 94 eye shadows samples available on the Polish market were collected. Eye shadow products have been selected in order to include several parameters important from the point of view of the typical consumer such as: product type (mat/pearl), consumer group (for adults and children), price range (very cheap, medium price, expensive and very expensive), color (twelve different colors were tested), manufacturer (eight brands were investigated) or country of production (four countries were included). The concentration of selected metals (Ag, Ba, Bi, Cd, Pb, Sr, Tl) was determined by ICP-MS technique after the sample extraction with a mixture of nitric acid and hydrogen peroxide in a microwave closed system. For Ag, Cd and Tl, some results were below the established limit of quantification for the employed technique. The presence of strontium, barium, lead and bismuth was confirmed in all studied samples. The obtained results for analyzed elements were, in general, quite comparable with the data reported by other authors. A small number of samples exceeding the permissible values (two samples were beyond the limit value for Cd of 0.5 mg/kg and one exceed the acceptable concentration for Pb of 10 mg/kg) also proves a relatively good condition of the Polish cosmetics market and suggests insubstantial risk for the potential consumers. The results gathered for some of the eye shadows intended for children turned out to be alarmingly high, in particular for elements such as Cd. The highest concentration of Cd reached almost 4 mg/kg, while of Pb amounted to 16 mg/kg. The presence of the statistically significant differences was confirmed for all included parameters with an exception of the color of the eye shadow. Considering the results acquired only for Cd and Pb with respect to the country of origin, the least contaminated cosmetics by metallic impurities seem to be the one produced in Canada, while the ones presenting the highest health risk among all studied eye shadows are make-up cosmetics originating from Poland and Italy. Multivariate analysis of a large data set using CA methods and PCA provided valuable information on dependencies between variables and objects.
Organosolv Fractionation of Birch Sawdust: Establishing a Lignin-First Biorefinery
Authors: Monção Maxwel , Hrůzová Kateřina , Rova Ulrika , Matsakas Leonidas , Christakopoulos Paul
Published: November 2021

The use of residual biomass for bioconversions makes it possible to decrease the output of fossil-based chemicals and pursue a greener economy. While the use of lignocellulosic material as sustainable feedstock has been tried at pilot scale, industrial production is not yet economically feasible, requiring further technology and feedstock optimization. The aim of this study was to examine the feasibility of replacing woodchips with residual sawdust in biorefinery applications. Woodchips can be used in value-added processes such as paper pulp production, whereas sawdust is currently used mainly for combustion. The main advantages of sawdust are its large supply and a particle size sufficiently small for the pretreatment process. Whereas, the main challenge is the higher complexity of the lignocellulosic biomass, as it can contain small amounts of bark and cambium. Here, we studied the fractionation of birch sawdust by organosolv pretreatment at two different temperatures and for two different durations. We evaluated the efficiency of fractionation into the three main fractions: lignin, cellulose, and hemicellulose. The cellulose content in pretreated biomass was as high as 69.2%, which was nearly double the amount in untreated biomass. The obtained lignin was of high purity, with a maximum 4.5% of contaminating sugars. Subsequent evaluation of the susceptibility of pretreated solids to enzymatic saccharification revealed glucose yields ranging from 75% to 90% after 48 h but reaching 100.0% under the best conditions. In summary, birch sawdust can be successfully utilized as a feedstock for organosolv fractionation and replace woodchips to simplify and lower the costs of biorefinery processes.
Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
Authors: Tantipaiboonwong Payungsak , Chaiwangyen Wittaya , Suttajit Maitree , Kangwan Napapan , Kaowinn Sirichat , Khanaree Chakkrit , Punfa Wanisa , Pintha Komsak
Published: November 2021

Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.
Ultrasound-Assisted Extraction of Carotenoids from Carrot Pomace and Their Optimization through Response Surface Methodology
Authors: Umair Muhammad , Jabbar Saqib , Nasiru Mustapha M. , Lu Zhaoxin , Zhang Jianhao , Abid Muhammad , Murtaza Mian Anjum , Kieliszek Marek , Zhao Liqing
Published: November 2021

Ultrasound-assisted extraction (UAE) was used to extract carotenoids from the carrot pomace. To investigate the effect of independent variables on the UAE, the response surface methodology (RSM) with central-composite design (CCD) was employed. The study was conducted with three independent variables including extraction time (min), temperature (°C), and ethanol concentration (%). The results showed that the optimal conditions for UAE were achieved with an extraction time of 17 min, temperature of 32 °C, and ethanol concentration of 51% of total carotenoids (31.82 ± 0.55); extraction time of 16 min, temperature of 29 °C, and ethanol concentration of 59% for a combination of β-carotene (14.89 ± 0.40), lutein (5.77 ± 0.19), and lycopene (2.65 ± 0.12). The non-significant (p > 0.05) correlation under optimal extraction conditions between predicted and experimental values suggested that UAE is the more productive process than conventional techniques for the extraction of carotenoids from the carrot pomace.
Physiological and Biochemical Parameters of Common Duckweed Lemna minor after the Exposure to Tetracycline and the Recovery from This Stress
Authors: Krupka Magdalena , Michalczyk Dariusz J. , Žaltauskaitė Jūratė , Sujetovienė Gintarė , Głowacka Katarzyna , Grajek Hanna , Wierzbicka Marta , Piotrowicz-Cieślak Agnieszka I.
Published: November 2021

In this study, the ability of Lemna minor L. to recover to normal growth, after being degraded in a tetracycline-containing medium, was extensively investigated. The plants were exposed to tetracycline (TC) at concentrations of 1, 2.5, and 10 mM. Subsequently, their physiological status was analysed against the following criteria: rate of plant growth; free radical accumulation; antioxidant enzyme activity; chlorophyll content; HSP70 protein content; cell membrane permeability, and mitochondrial activity. The study showed that duckweed can considerably recover from the damage caused by antibiotics, within a week of cessation of stress. Of the plant properties analysed, mitochondrial activity was the most sensitive to antibiotic-induced disturbances. After transferring the plants to a tetracycline-free medium, all plant parameters improved significantly, except for the mitochondrial activity in the plants grown on the medium containing the highest dose of tetracycline. In the plants treated with this antibiotic at the concentration of 10 mM, the proportion of dead mitochondria increased and was as high as 93% after one week from the beginning of the recovery phase, even after the transfer to the tetracycline-free medium.
Special Low Protein Foods Prescribed in England for PKU Patients: An Analysis of Prescribing Patterns and Cost
Authors: Wood Georgina , Pinto Alex , Evans Sharon , Daly Anne , Adams Sandra , Costelloe Susie , Gribben Joanna , Ellerton Charlotte , Emm Anita , Firman Sarah , Ford Suzanne , French Moira , Gaff Lisa , Giuliano Emily , Hill Melanie , Hunjan Inderdip , Newby Camille , Mackenzie Allison , Pereira Rachel , Prescott Celine , Robertson Louise , Seabert Heidi , Skeath Rachel , Tapley Simon , Terry Allyson , Tooke Alison , van Wyk Karen , White Fiona J. , White Lucy , Woodall Alison , Rocha Júlio César , MacDonald Anita
Published: November 2021

Patients with phenylketonuria (PKU) are reliant on special low protein foods (SLPFs) as part of their dietary treatment. In England, several issues regarding the accessibility of SLPFs through the national prescribing system have been highlighted. Therefore, prescribing patterns and expenditure on all SLPFs available on prescription in England (n = 142) were examined. Their costs in comparison to regular protein-containing (n = 182) and ‘free-from’ products (n = 135) were also analysed. Similar foods were grouped into subgroups (n = 40). The number of units and costs of SLPFs prescribed in total and per subgroup from January to December 2020 were calculated using National Health Service (NHS) Business Service Authority (NHSBSA) ePACT2 (electronic Prescribing Analysis and Cost Tool) for England. Monthly patient SLPF units prescribed were calculated using patient numbers with PKU and non-PKU inherited metabolic disorders (IMD) consuming SLPFs. This was compared to the National Society for PKU (NSPKU) prescribing guidance. Ninety-eight percent of SLPF subgroups (n = 39/40) were more expensive than regular and ‘free-from’ food subgroups. However, costs to prescribe SLPFs are significantly less than theoretical calculations. From January to December 2020, 208,932 units of SLPFs were prescribed (excluding milk replacers), costing the NHS £2,151,973 (including milk replacers). This equates to £962 per patient annually, and prescribed amounts are well below the upper limits suggested by the NSPKU, indicating under prescribing of SLPFs. It is recommended that a simpler and improved system should be implemented. Ideally, specialist metabolic dietitians should have responsibility for prescribing SLPFs. This would ensure that patients with PKU have the necessary access to their essential dietary treatment, which, in turn, should help promote dietary adherence and improve metabolic control.
Potential of Vitamin D Food Fortification in Prevention of Cancer Deaths—A Modeling Study
Authors: Niedermaier Tobias , Gredner Thomas , Kuznia Sabine , Schöttker Ben , Mons Ute , Brenner Hermann
Published: November 2021

Meta-analyses of randomized controlled trials (RCTs) have estimated a 13% reduction of cancer mortality by vitamin D supplementation among older adults. We evaluated if and to what extent similar effects might be expected from vitamin D fortification of foods. We reviewed the literature on RCTs assessing the impact of vitamin D supplementation on cancer mortality, on increases of vitamin D levels by either supplementation or food fortification, and on costs of supplementation or fortification. Then, we derived expected effects on total cancer mortality and related costs and savings from potential implementation of vitamin D food fortification in Germany and compared the results to those for supplementation. In RCTs with vitamin D supplementation in average doses of 820–2000 IU per day, serum concentrations of 25-hydroxy-vitamin D increased by 15–30 nmol/L, respectively. Studies on food fortification found increases by 10–42 nmol/L, thus largely in the range of increases previously demonstrated by supplementation. Fortification is estimated to be considerably less expensive than supplementation. It might be similarly effective as supplementation in reducing cancer mortality and might even achieve such reduction at substantially larger net savings. Although vitamin D overdoses are unlikely in food fortification programs, implementation should be accompanied by a study monitoring the frequency of potentially occurring adverse effects by overdoses, such as hypercalcemia. Future studies on effectiveness of vitamin D supplementation and fortification are warranted.
Vitamin A Transporters in Visual Function: A Mini Review on Membrane Receptors for Dietary Vitamin A Uptake, Storage, and Transport to the Eye
Authors: Martin Ask Nicasio , Leung Matthias , Radhakrishnan Rakesh , Lobo Glenn P.
Published: November 2021

Vitamins are essential compounds obtained through diet that are necessary for normal development and function in an organism. One of the most important vitamins for human physiology is vitamin A, a group of retinoid compounds and carotenoids, which generally function as a mediator for cell growth, differentiation, immunity, and embryonic development, as well as serving as a key component in the phototransduction cycle in the vertebrate retina. For humans, vitamin A is obtained through the diet, where provitamin A carotenoids such as β-carotene from plants or preformed vitamin A such as retinyl esters from animal sources are absorbed into the body via the small intestine and converted into all-trans retinol within the intestinal enterocytes. Specifically, once absorbed, carotenoids are cleaved by carotenoid cleavage oxygenases (CCOs), such as Beta-carotene 15,15’-monooxygenase (BCO1), to produce all-trans retinal that subsequently gets converted into all-trans retinol. CRBP2 bound retinol is then converted into retinyl esters (REs) by the enzyme lecithin retinol acyltransferase (LRAT) in the endoplasmic reticulum, which is then packaged into chylomicrons and sent into the bloodstream for storage in hepatic stellate cells in the liver or for functional use in peripheral tissues such as the retina. All-trans retinol also travels through the bloodstream bound to retinol binding protein 4 (RBP4), where it enters cells with the assistance of the transmembrane transporters, stimulated by retinoic acid 6 (STRA6) in peripheral tissues or retinol binding protein 4 receptor 2 (RBPR2) in systemic tissues (e.g., in the retina and the liver, respectively). Much is known about the intake, metabolism, storage, and function of vitamin A compounds, especially with regard to its impact on eye development and visual function in the retinoid cycle. However, there is much to learn about the role of vitamin A as a transcription factor in development and cell growth, as well as how peripheral cells signal hepatocytes to secrete all-trans retinol into the blood for peripheral cell use. This article aims to review literature regarding the major known pathways of vitamin A intake from dietary sources into hepatocytes, vitamin A excretion by hepatocytes, as well as vitamin A usage within the retinoid cycle in the RPE and retina to provide insight on future directions of novel membrane transporters for vitamin A in retinal cell physiology and visual function.
Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice
Authors: Yang Mo , Zheng Jiapeng , Zong Xinran , Yang Xinyan , Zhang Yu , Man Chaoxin , Jiang Yujun
Published: November 2021

Probiotics can prevent obesity and related metabolic complications. In our study, the protective effect and molecular mechanism of Lactobacillus rhamnosus JL1 (separated from the feces of healthy infants) on high-fat diet mice were investigated. After 10 weeks of dietary intervention with L. rhamnosus JL1 intervention, the body weight of the JL1 group (23.78 g) was significantly lower than that of the HFD group (26.59 g, p < 0.05) and the liver index was reduced. Serum biochemical analysis showed that the TC, TG and LDL-C contents of JL1 group mice were significantly decreased (p < 0.05). Histological images of the mice livers showed that the degree of lipid action and damage of hepatic cells were improved. L. rhamnosus JL1 activated the AMPK pathway, and reduced the gene expression of PPAR-γ, LXR-α and SREBP-1C. In addition, the protein expression of PPAR-γ and LXR-α were reduced. After dietary intervention with L. rhamnosus JL1, the concentration of acetic acid, propionic acid, and butyric acid were increased significantly, especially the concentration of butyric acid, which was 63.16% higher than that of the HFD group (p < 0.05). In conclusion, this study provided a theoretical reference for the development and application of probiotics derived from healthy infant feces in health products and functional foods.
Pre-Treatment C-Reactive Protein Predicts Survival in Small Cell Lung Cancer Patients
Authors: Stensvold Anne Marie , Aggerholm-Pedersen Ninna , Winther-Larsen Anne , Sandfeld-Paulsen Birgitte
Published: November 2021

Improved prognostication of small cell lung cancer (SCLC) patients could strengthen the treatment strategy and, thereby, potentially improve the overall survival (OS) of these patients. C-reactive protein (CRP) has been proposed as a prognostic indicator of inferior survival, although so far, only based on data from smaller studies. Data on SCLC patients diagnosed from January 2009 to June 2018 were extracted from the Danish Lung Cancer Registry and the clinical laboratory information system. CRP measurements were divided at the clinical cut-off value of 8 mg/L or 75 nmol/L) and stratified into quartiles. Cox proportional hazards model assessed the prognostic value of the CRP level. C-statistics further evaluated the biomarker’s prognostic value. In total, 923 patients were included. A pre-treatment CRP level above the clinical cut-off significantly correlated to inferior OS (adjusted hazard ratio (HR) = 1.25 (95% confidence interval (CI): 1.08–1.46). When divided into quartiles, a level-dependent correlation was observed with only the highest quartiles significantly associated with OS (3rd quartile: adjusted HR = 1.26 (95% CI: 1.03–1.55) 4th quartile: adjusted HR = 1.44 (95% CI: 1.17–1.77)). Adding CRP level to already well-established prognostic factors improved the prognostication of SCLC patients. In conclusion, high pre-treatment CRP level is an independent prognostic factor in SCLC patients.
Mycoplasma genitalium Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
Authors: Dai Pei , Deng Xiangying , Liu Peng , Li Lingling , Luo Dan , Liao Yating , Zeng Yanhua
Published: November 2021

Mycoplasma genitalium is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of Mycoplasma genitalium, is the main adhesin and the key factor for M. genitalium interacting with host cells. Currently, the long-term survival mechanism of M. genitalium in the host is not clear. In this study, a T7 phage-displayed human urothelial cell (SV-HUC-1) cDNA library was constructed, and the interaction of MgPa was screened from this library using the recombinant MgPa (rMgPa) as a target molecule. We verified that 60S ribosomal protein L35 (RPL35) can interact with MgPa using far-Western blot and co-localization analysis. According to the results of tandem mass tag (TMT) labeling and proteome quantitative analysis, there were altogether 407 differentially expressed proteins between the pcDNA3.1(+)/MgPa-transfected cells and non-transfected cells, of which there were 6 downregulated proteins and 401 upregulated proteins. The results of qRT-PCR demonstrated that interaction between rMgPa and RPL35 could promote the expressions of EIF2, SRP68, SERBP1, RPL35A, EGF, and TGF-β. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide (MTT) assays corroborated that the interaction between rMgPa and RPL35 could promote SV-HUC-1 cell proliferation. Therefore, our findings indicated that the interaction between rMgPa and RPL35 can enhance the expressions of transcription-initiation and translation-related proteins and thus promote cell proliferation. This study elucidates a new biological function of MgPa and can explain this new mechanism of M. genitalium in the host.
Regulation of m6A Methylation as a New Therapeutic Option against COVID-19
Authors: Zannella Carla , Rinaldi Luca , Boccia Giovanni , Chianese Annalisa , Sasso Ferdinando Carlo , De Caro Francesco , Franci Gianluigi , Galdiero Massimiliano
Published: November 2021

The rapid spread of SARS-CoV-2 and the resulting pandemic has led to a spasmodic search for approaches able to limit the diffusion of the disease. The epigenetic machinery has aroused considerable interest in the last decades, and much evidence has demonstrated that this type of modification could regulate the early stages of viral infection. Recently it was reported that N6-methyladenosine (m6A) influences SARS-CoV-2 replication, although its role remains to be further investigated. The knockdown of enzymes involved in the m6A pathway could represent an optimal strategy to deepen the epigenetic mechanism. In the present study, we blocked the catalytic activity of the fat mass and obesity-associated protein (FTO) by using the selective inhibitor rhein. We observed a strong broad-spectrum reduction of infectivity caused by various coronaviruses, including SARS-CoV-2. This effect could be due to the modulation of m6A levels and could allow identification of this modification as a new therapeutic target to treat SARS-CoV-2 infection.
Effective Treatment against ESBL-Producing Klebsiella pneumoniae through Synergism of the Photodynamic Activity of Re (I) Compounds with Beta-Lactams
Authors: González Iván A. , Palavecino Annegrett , Núñez Constanza , Dreyse Paulina , Melo-González Felipe , Bueno Susan M. , Palavecino Christian Erick
Published: November 2021

Background: Extended-spectrum beta-lactamase (ESBL) and carbapenemase (KPC+) producing Klebsiella pneumoniae are multidrug-resistant bacteria (MDR) with the highest risk to human health. The significant reduction of new antibiotics development can be overcome by complementing with alternative therapies, such as antimicrobial photodynamic therapy (aPDI). Through photosensitizer (PS) compounds, aPDI produces local oxidative stress-activated by light (photooxidative stress), nonspecifically killing bacteria. Methodology: Bimetallic Re(I)-based compounds, PSRe-µL1 and PSRe-µL2, were tested in aPDI and compared with a Ru(II)-based PS positive control. The ability of PSRe-µL1 and PSRe-µL2 to inhibit K. pneumoniae was evaluated under a photon flux of 17 µW/cm2. In addition, an improved aPDI effect with imipenem on KPC+ bacteria and a synergistic effect with cefotaxime on ESBL producers of a collection of 118 clinical isolates of K. pneumoniae was determined. Furthermore, trypan blue exclusion assays determined the PS cytotoxicity on mammalian cells. Results: At a minimum dose of 4 µg/mL, both the PSRe-µL1 and PSRe-µL2 significantly inhibited in 3log10 (>99.9%) the bacterial growth and showed a lethality of 60 and 30 min of light exposure, respectively. Furthermore, they were active on clinical isolates of K. pneumoniae at 3–6 log10. Additionally, a remarkably increased effectiveness of aPDI was observed over KPC+ bacteria when mixed with imipenem, and a synergistic effect from 3 to 6log10 over ESBL producers of K. pneumoniae clinic isolates when mixed with cefotaxime was determined for both PSs. Furthermore, the compounds show no dark toxicity and low light-dependent toxicity in vitro to mammalian HEp-2 and HEK293 cells. Conclusion: Compounds PSRe-µL1 and PSRe-µL2 produce an effective and synergistic aPDI effect on KPC+, ESBL, and clinical isolates of K. pneumoniae and have low cytotoxicity in mammalian cells.
Nanotechnology Applications of Flavonoids for Viral Diseases
Authors: Jannat Khoshnur , Paul Alok K. , Bondhon Tohmina A. , Hasan Anamul , Nawaz Muhammad , Jahan Rownak , Mahboob Tooba , Nissapatorn Veeranoot , Wilairatana Polrat , Pereira Maria de Lourdes , Rahmatullah Mohammed
Published: November 2021

Recent years have witnessed the emergence of several viral diseases, including various zoonotic diseases such as the current pandemic caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Other viruses, which possess pandemic-causing potential include avian flu, Ebola, dengue, Zika, and Nipah virus, as well as the re-emergence of SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome) coronaviruses. Notably, effective drugs or vaccines against these viruses are still to be discovered. All the newly approved vaccines against the SARS-CoV-2-induced disease COVID-19 possess real-time possibility of becoming obsolete because of the development of ‘variants of concern’. Flavonoids are being increasingly recognized as prophylactic and therapeutic agents against emerging and old viral diseases. Around 10,000 natural flavonoid compounds have been identified, being phytochemicals, all plant-based. Flavonoids have been reported to have lesser side effects than conventional anti-viral agents and are effective against more viral diseases than currently used anti-virals. Despite their abundance in plants, which are a part of human diet, flavonoids have the problem of low bioavailability. Various attempts are in progress to increase the bioavailability of flavonoids, one of the promising fields being nanotechnology. This review is a narrative of some anti-viral dietary flavonoids, their bioavailability, and various means with an emphasis on the nanotechnology system(s) being experimented with to deliver anti-viral flavonoids, whose systems show potential in the efficient delivery of flavonoids, resulting in increased bioavailability.
Carotenoids from Persimmon (Diospyros kaki Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
Authors: Gea-Botella Sara , Moreno-Chamba Bryan , de la Casa Laura , Salazar-Bermeo Julio , Martí Nuria , Martínez-Madrid María Concepción , Valero Manuel , Saura Domingo
Published: November 2021

Persimmon (Diospyros kaki Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in carotenoids and obtained from byproducts derived from the persimmon juice industry, against UV-induced cell death in the keratinocyte HaCaT cell line. For this purpose, the cytotoxicity and phototoxicity of carotenoid extract, as well as its intracellular reactive oxygen species (ROS) scavenging and anti-adhesive activities towards HaCaT cells, were evaluated. The in vitro permeation test provided information about the permeability of the carotenoid extract. Persimmon extracts, rich in carotenoids (PEC), were absorbed by HaCaT keratinocyte cells, which reduced the UV-induced intracellular ROS production in treated cells. Thus, PEC exerted a photoprotective and regenerative effect on UV-irradiated HaCaT cells, and this protection was UV dose-dependent. No cytotoxic effect was observed in HaCaT cultures at the concentration tested. PEC treatment also stimulated the adhesion capacity of skin microbiome to HaCaT cells, while exhibiting a significant anti-adhesive activity against all tested pathogens. In conclusion, PEC showed potential for use as a functional ingredient in skin-care products.