bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Internet Addiction on Academic Performance Among Medical Students in Bangladesh: A Cross-Sectional Study and the Potential Role of Yoga#
Authors: Afrin Sumaiya , Rahman Nur-A-Safrina , Tabassum Tahsin Tasneem , Abdullah Faisal , Rahman Md. Istiakur , Simu Sumona Haque , Kumar Lakshya , Noor Khutaija , Vishal FNU , Podder Vivek
Published: September 2024

Background

Excessive internet use is a growing concern globally, and internet addiction negatively impacts academic performance. Limited research has been conducted on this topic among undergraduate medical students in Bangladesh.

Purpose

This study aimed to determine the prevalence of internet addiction and its impact on academic performance among medical students in Bangladesh, with a focus on the role of yoga in reducing Internet addiction as a proposed intervention.

Methods

A cross-sectional study was conducted among third-year medical students in four colleges in Dhaka, Bangladesh. Simple random sampling techniques were used to select participants, and data were collected using pretested questionnaires and a checklist for office records through face-to-face interviews. SPSS version 25 was used for data processing and analysis.

Results

Out of 312 students, 84% were addicted to the internet, and only 16% were normal. In total, 64.4% had good results in the first professional examination, while 35.6% had poor results. In total, 85.9% had good attendance, while 14.1% had poor attendance. The association between internet addiction level and first professional examination results and class attendance was highly significant.

Conclusion

This study highlights the high prevalence and negative impacts of internet addiction among medical students in Bangladesh. The study suggests that promoting awareness about healthy technology use, establishing a better balance between internet usage and academic study, and encouraging the practice of yoga can help alleviate this problem. Further research and interventions are needed to tackle this emerging public health issue. Encouraging students to use the internet for academic purposes and providing education and resources can help promote healthy technology use. Healthcare professionals should also be aware of the risks and encourage seeking help if needed.
A qualitative study on the experiences of women undergoing surgery for developmental breast asymmetry
Authors: Ho Lorraine Kit Ying , Jafari Sahima , Crittenden Tamara , van Essen Phillipa , Smallman Andrea , Dean Nicola R.
Published: September 2024

Background:

Developmental breast asymmetry (DBA) is a largely underreported condition where the natural growth of one breast is smaller than the other. While some degree of asymmetry or difference in size and shape is present in most women, DBA can result in more profound differences that can impact a woman’s psychosocial well-being.

Objectives:

This study aims to better understand the experiences of women living with DBA, their experiences seeking treatment, and their reconstructive surgical journey and outcomes.

Design:

This was a qualitative study involving in-depth, one-on-one semi-structured interviews with women diagnosed with DBA.

Methods:

Participants were women seeking treatment for DBA through the Plastic and Reconstructive Surgery Unit at Flinders Medical Centre, a tertiary healthcare centre in Adelaide, South Australia. Interviews were recorded digitally, transcribed verbatim and analysed thematically.

Results:

Fourteen interviews were conducted with 14 women; 13 women had completed their reconstruction and 1 was undergoing reconstruction at the time of their interview. Interviews highlighted the significant psychosocial impact of DBA, the different experiences in seeking help for DBA, the information received or lack thereof, the need for medical and social support throughout the surgical process, and the varied satisfaction with surgical outcomes.

Conclusion:

This study highlighted the subjective experiences of women who have grown up with DBA, improving our understanding of the significant psychosocial impact of DBA. Not all participants experienced post-operative improvements in psychosocial well-being due to surgical complications or unmet expectations. This study also demonstrated the need to raise awareness about DBA and the importance of additional medical and social support for women throughout their surgical journey.
Increasing Identification and Follow-Up of Cognitive Impairment in Primary Care: A Quality Improvement Pilot Study
Authors: Uomoto Keirstin E.
Published: September 2024

Introduction:

Beyond our population growing older and living longer, there is an increased risk of developing a cognitive disorder. Standardized screening during a routine visit in primary care may be ideal for early detection of mild cognitive impairment (MCI) and follow-up for cognitive changes.

Aim:

This quality improvement (QI) project aimed to determine the impact of implementing the Mini-Cog© quick screening for early dementia detection to identify and follow up on the cognitive impairment of older adults in a primary care clinic setting.

Methods:

Implementation started in February 2024 in a primary care clinic in Southern California. Data was collected for this project over a total of 16 weeks. This QI project implemented a routine cognitive screening using the Mini-Cog©. Cognitive impairment was identified, and if indicated by the Mini-Cog© scores, follow-up for a cognitive assessment and care plan services were initiated. Data were obtained from the project site’s electronic medical record on a total sample size of 471 participants (n = 382 in the pre-implementation group and n = 89 in the post-implementation group).

Results:

Pearson’s chi-square test indicated a statistically significant improvement in the identification rate of cognitive impairment, increasing from 11.8% (n = 45 out of 382) at pre-implementation to 34.8% (n = 31 out of 89) at post-implementation, and specifically, mild cognitive impairment increased from zero identified in pre-implementation to 12.4% (n = 11 out of 89) post-implementation. Lastly, follow-up rates improved from 91.1% (n = 41 out of 45) to 100% (n = 31 out of 31) in post-implementation, and clinical significance was evident based on the phi-coefficient ( φ = 0.196), indicating a small effect size and a 100% follow-up rate.

Conclusions:

The findings of this project suggest older adults should receive cognitive screenings to help identify early cognitive impairment and increase follow-up for further evaluation, treatment, and advanced care planning.
Soil pH enhancement and alterations in nutrient and Bacterial Community profiles following Pleioblastus amarus expansion in tea plantations
Authors: Fan Lili , Chen Shuanglin , Guo Ziwu , Hu Ruicai , Yao Liangjin
Published: September 2024

Background

The expansion of bamboo forests increases environmental heterogeneity in tea plantation ecosystems, affecting soil properties and microbial communities. Understanding these impacts is essential for developing sustainable bamboo management and maintaining ecological balance in tea plantations.

Methods

We studied the effect of the continuous expansion of Pleioblastus amarus into tea plantations, by establishing five plot types: pure P. amarus forest area (BF), P. amarus forest interface area (BA), mixed forest interface area (MA), mixed forest center area (TB), and pure tea plantation area (TF). We conducted a comprehensive analysis of soil chemical properties and utilized Illumina sequencing to profile microbial community composition and diversity, emphasizing their responses to bamboo expansion.

Results

(1) Bamboo expansion significantly raised soil pH and enhanced levels of organic matter, nitrogen, and phosphorus, particularly noticeable in BA and MA sites. In the TB sites, improvements in soil nutrients were statistically indistinguishable from those in pure tea plantation areas. (2) Continuous bamboo expansion led to significant changes in soil bacterial diversity, especially noticeable between BA and TF sites, while fungal diversity was unaffected. (3) Bamboo expansion substantially altered the composition of less abundant bacterial and fungal communities, which proved more sensitive to changes in soil chemical properties.

Conclusion

The expansion of bamboo forests causes significant alterations in soil pH and nutrient characteristics, impacting the diversity and composition of soil bacteria in tea plantations. However, as expansion progresses, its long-term beneficial impact on soil quality in tea plantations appears limited.
Hypnosis support in anaesthesia is rarely used in German anaesthesia departments - a nationwide survey among leading physicians of anaesthesia departments
Authors: Bügers Lisa , Wähner Anna , Schubert Ann-Kristin , Dinges Hanns-Christian , Torossian Alexander , Volberg Christian
Published: September 2024

Background

The aim of this study was to investigate whether and to what extent perioperative hypnosis and relaxation techniques are used in German anaesthesia departments, what they are, where any difficulties in their application lie and how great the interest in this type of therapy is. Another research question was to find out whether there are specialist areas in which these methods are used more frequently than in other specialist areas.

Methods

A descriptive survey was conducted by means of a questionnaire in all hospitals with anaesthesia departments in Germany. 1124 questionnaires were sent out by post. The survey period was five months from 27/02/2023 to 31/07/2023. The clinic directors of all anaesthesiology departments in German hospitals were surveyed.

Results

476 departments (42%) responded by pre-paid envelope. Of these, only 39 (8%) use hypnosis and relaxation techniques perioperatively. These are mostly progressive muscle relaxation, hypnotic trance according to Erickson, calming words and suggestions or the use of virtual reality (e.g. using VR-glasses).

Conclusions

Hypnosis techniques have been shown in many studies to be effective in increasing patient comfort (less anxiety and stress) and reducing both the need for medication and perioperative pain. The therapy is rarely used in Germany, although once established it can be easily integrated into perioperative procedures. Many departments have shown great interest in the topic. In the interests of patients, a structural change should be considered to promote the use of hypnotic procedures.
HSF1 is a prognostic determinant and therapeutic target in intrahepatic cholangiocarcinoma
Authors: Cigliano Antonio , Gigante Isabella , Serra Marina , Vidili Gianpaolo , Simile Maria M. , Steinmann Sara , Urigo Francesco , Cossu Eleonora , Pes Giovanni M. , Dore Maria P. , Ribback Silvia , Milia Egle P. , Pizzuto Elena , Mancarella Serena , Che Li , Pascale Rosa M. , Giannelli Gianluigi , Evert Matthias , Chen Xin , Calvisi Diego F.
Published: September 2024

Background

Intrahepatic cholangiocarcinoma (iCCA) is a lethal primary liver tumor characterized by clinical aggressiveness, poor prognosis, and scarce therapeutic possibilities. Therefore, new treatments are urgently needed to render this disease curable. Since cumulating evidence supports the oncogenic properties of the Heat Shock Factor 1 (HSF1) transcription factor in various cancer types, we investigated its pathogenetic and therapeutic relevance in iCCA.

Methods

Levels of HSF1 were evaluated in a vast collection of iCCA specimens. The effects of HSF1 inactivation on iCCA development in vivo were investigated using three established oncogene-driven iCCA mouse models. In addition, the impact of HSF1 suppression on tumor cells and tumor stroma was assessed in iCCA cell lines, human iCCA cancer-associated fibroblasts (hCAFs), and patient-derived organoids.

Results

Human preinvasive, invasive, and metastatic iCCAs displayed widespread HSF1 upregulation, which was associated with a dismal prognosis of the patients. In addition, hydrodynamic injection of a dominant-negative form of HSF1 (HSF1dn), which suppresses HSF1 activity, significantly delayed cholangiocarcinogenesis in AKT/NICD, AKT/YAP, and AKT/TAZ mice. In iCCA cell lines, iCCA hCAFs, and patient-derived organoids, administration of the HSF1 inhibitor KRIBB-11 significantly reduced proliferation and induced apoptosis. Cell death was profoundly augmented by concomitant administration of the Bcl-xL/Bcl2/Bcl-w inhibitor ABT-263. Furthermore, KRIBB-11 reduced mitochondrial bioenergetics and glycolysis of iCCA cells.

Conclusions

The present data underscore the critical pathogenetic, prognostic, and therapeutic role of HSF1 in cholangiocarcinogenesis.
Effect of a soft exosuit on daily life gait performance in people with incomplete spinal cord injury: study protocol for a randomized controlled trial
Authors: Visch L. , Groen B. E. , Geurts A. C. H. , van Nes I. J. W. , Keijsers N. L. W.
Published: September 2024

Background

People with incomplete spinal cord injury (iSCI) often have gait impairments that negatively affect daily life gait performance (i.e., ambulation in the home and community setting) and quality of life. They may benefit from light-weight lower extremity exosuits that assist in walking, such as the Myosuit (MyoSwiss AG, Zurich, Switzerland). A previous pilot study showed that participants with various gait disorders increased their gait speed with the Myosuit in a standardized environment. However, the effect of a soft exosuit on daily life gait performance in people with iSCI has not yet been evaluated.

Objective

The primary study objective is to test the effect of a soft exosuit (Myosuit) on daily life gait performance in people with iSCI. Second, the effect of Myosuit use on gait capacity and the usability of the Myosuit in the home and community setting will be investigated. Finally, short-term impact on both costs and effects will be evaluated.

Methods

This is a two-armed, open label, randomized controlled trial (RCT). Participants will be randomized (1:1) to the intervention group (receiving the Myosuit program) or control group (initially receiving the conventional program). Thirty-four people with chronic iSCI will be included. The Myosuit program consists of five gait training sessions with the Myosuit at the Sint Maartenskliniek. Thereafter, participants will have access to the Myosuit for home use during 6 weeks. The conventional program consists of four gait training sessions, followed by a 6-week home period. After completing the conventional program, participants in the control group will subsequently receive the Myosuit program. The primary outcome is walking time per day as assessed with an activity monitor at baseline and during the first, third, and sixth week of the home periods. Secondary outcomes are gait capacity (10MWT, 6MWT, and SCI-FAP), usability (D-SUS and D-QUEST questionnaires), and costs and effects (EQ-5D-5L).

Discussion

This is the first RCT to investigate the effect of the Myosuit on daily life gait performance in people with iSCI.

Trial registration

Clinicaltrials.gov NCT05605912. Registered on November 2, 2022.
High fidelity adaptive mirror simulations with reduced order models
Authors: Stadler Bernadett , Biasi Roberto , Manetti Mauro , Obereder Andreas , Ramlau Ronny , Tintori Matteo
Published: September 2024

In the design process of large adaptive mirrors numerical simulations represent the first step to evaluate the system design compliance in terms of performance, stability and robustness. For the next generation of Extremely Large Telescopes increased system dimensions and bandwidths lead to the need of modeling not only the deformable mirror alone, but also all the system supporting structure or even the full telescope. The capability to perform the simulations with an acceptable amount of time and computational resources is highly dependent on finding appropriate methods to reduce the size of the resulting dynamic models. In this paper we present a framework developed together with the company Microgate to create a reduced order structural model of a large adaptive mirror as a preprocessing step to the control system simulations. The reduced dynamic model is then combined with the remaining system components allowing to simulate the full adaptive mirror in a computationally efficient way. We analyze the feasibility of our reduced models for Microgate’s prototype of the adaptive mirror of the Giant Magellan Telescope.
Targeting stress induction of GRP78 by cardiac glycoside oleandrin dually suppresses cancer and COVID-19
Authors: Ha Dat P. , Shin Woo-Jin , Liu Ze , Doche Michael E. , Lau Roy , Leli Nektaria Maria , Conn Crystal S. , Russo Mariangela , Lorenzato Annalisa , Koumenis Constantinos , Yu Min , Mumenthaler Shannon M. , Lee Amy S.
Published: September 2024

Background

Despite recent therapeutic advances, combating cancer resistance remains a formidable challenge. The 78-kilodalton glucose-regulated protein (GRP78), a key stress-inducible endoplasmic reticulum (ER) chaperone, plays a crucial role in both cancer cell survival and stress adaptation. GRP78 is also upregulated during SARS-CoV-2 infection and acts as a critical host factor. Recently, we discovered cardiac glycosides (CGs) as novel suppressors of GRP78 stress induction through a high-throughput screen of clinically relevant compound libraries. This study aims to test the possibility that agents capable of blocking stress induction of GRP78 could dually suppress cancer and COVID-19.

Results

Here we report that oleandrin (OLN), is the most potent among the CGs in inhibiting acute stress induction of total GRP78, which also results in reduced cell surface and nuclear forms of GRP78 in stressed cells. The inhibition of stress induction of GRP78 is at the post-transcriptional level, independent of protein degradation and autophagy and may involve translational control as OLN blocks stress-induced loading of ribosomes onto GRP78 mRNAs. Moreover, the human Na+/K+-ATPase α3 isoform is critical for OLN suppression of GRP78 stress induction. OLN, in nanomolar range, enhances apoptosis, sensitizes colorectal cancer cells to chemotherapeutic agents, and reduces the viability of patient-derived colon cancer organoids. Likewise, OLN, suppresses GRP78 expression and impedes tumor growth in an orthotopic breast cancer xenograft model. Furthermore, OLN blocks infection by SARS-CoV-2 and its variants and enhances existing anti-viral therapies. Notably, GRP78 overexpression mitigates OLN-mediated cancer cell apoptotic onset and suppression of virus release.

Conclusion

Our findings validate GRP78 as a target of OLN anti-cancer and anti-viral activities. These proof-of-principle studies support further investigation of OLN as a readily accessible compound to dually combat cancer and COVID-19.
Is artificial intelligence for medical professionals serving the patients?
Authors: Wilhelm Christoph , Steckelberg Anke , Rebitschek Felix G.
Published: September 2024

Background

Algorithmic decision-making (ADM) utilises algorithms to collect and process data and develop models to make or support decisions. Advances in artificial intelligence (AI) have led to the development of support systems that can be superior to medical professionals without AI support in certain tasks. However, whether patients can benefit from this remains unclear. The aim of this systematic review is to assess the current evidence on patient-relevant benefits and harms, such as improved survival rates and reduced treatment-related complications, when healthcare professionals use ADM systems (developed using or working with AI) compared to healthcare professionals without AI-related ADM (standard care)—regardless of the clinical issues.

Methods

Following the PRISMA statement, MEDLINE and PubMed (via PubMed), Embase (via Elsevier) and IEEE Xplore will be searched using English free text terms in title/abstract, Medical Subject Headings (MeSH) terms and Embase Subject Headings (Emtree fields). Additional studies will be identified by contacting authors of included studies and through reference lists of included studies. Grey literature searches will be conducted in Google Scholar. Risk of bias will be assessed by using Cochrane’s RoB 2 for randomised trials and ROBINS-I for non-randomised trials. Transparent reporting of the included studies will be assessed using the CONSORT-AI extension statement. Two researchers will screen, assess and extract from the studies independently, with a third in case of conflicts that cannot be resolved by discussion.

Discussion

It is expected that there will be a substantial shortage of suitable studies that compare healthcare professionals with and without ADM systems concerning patient-relevant endpoints. This can be attributed to the prioritisation of technical quality criteria and, in some cases, clinical parameters over patient-relevant endpoints in the development of study designs. Furthermore, it is anticipated that a significant portion of the identified studies will exhibit relatively poor methodological quality and provide only limited generalisable results.

Systematic review registration

This study is registered within PROSPERO (CRD42023412156).
Neurodegenerative disorders, metabolic icebergs, and mitohormesis
Authors: Phillips Matthew C. L. , Picard Martin
Published: September 2024

Neurodegenerative disorders are typically “split” based on their hallmark clinical, anatomical, and pathological features, but they can also be “lumped” by a shared feature of impaired mitochondrial biology. This leads us to present a scientific framework that conceptualizes Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington’s disease (HD) as “metabolic icebergs” comprised of a tip, a bulk, and a base. The visible tip conveys the hallmark neurological symptoms, neurodegenerative regions, and neuronal protein aggregates for each disorder. The hidden bulk depicts impaired mitochondrial biology throughout the body, which is multifaceted and may be subdivided into impaired cellular metabolism, cell-specific mitotypes, and mitochondrial behaviours, functions, activities, and features. The underlying base encompasses environmental factors, especially modern industrial toxins, dietary lifestyles, and cognitive, physical, and psychosocial behaviours, but also accommodates genetic factors specific to familial forms of AD, PD, and ALS, as well as HD. Over years or decades, chronic exposure to a particular suite of environmental and genetic factors at the base elicits a trajectory of impaired mitochondrial biology that maximally impacts particular subsets of mitotypes in the bulk, which eventually surfaces as the hallmark features of a particular neurodegenerative disorder at the tip. We propose that impaired mitochondrial biology can be repaired and recalibrated by activating “mitohormesis”, which is optimally achieved using strategies that facilitate a balanced oscillation between mitochondrial stressor and recovery phases. Sustainably harnessing mitohormesis may constitute a potent preventative and therapeutic measure for people at risk of, or suffering with, neurodegenerative disorders.
Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profile
Authors: Lv Yujie , Ge Chaoyue , Wu Lianchi , Hu Zhaoying , Luo Xinyu , Huang Weichen , Zhan Shenao , Shen Xinyu , Yu Dongyou , Liu Bing
Published: September 2024

Background

Magnolol (MAG) exhibits hepatoprotective activity, however, whether and how MAG regulates the gut microbiota to alleviate fatty liver hemorrhagic syndrome (FLHS) remains unclear. Therefore, we investigated the mechanism of MAG in FLHS laying hens with an emphasis on alterations in the gut–liver axis. We randomly divided 540 56-week-old Hy-line white laying hens with FLSH into 4 groups. The birds were fed a high-fat low-protein (HFLP) diet (CON) or HELP diets supplemented with 200, 400, and 600 mg/kg of MAG (M1, M2, and M3, respectively) for 9 weeks.

Results

Magnolol supplementation increased the laying rate and ameliorated hepatic damage and dysfunction by regulating lipid metabolism, improving intestinal barrier function, and shaping the gut microbiota and tryptophan metabolic profiles. Dietary MAG supplementation downregulated the expression of lipid synthesis genes and upregulated the expression of lipid transport genes at varying degrees. The intestinal barrier function was improved by 200 and 400 mg/kg of MAG supplementation, as evidenced by the increased villus height and mRNA expression of tight junction related genes. Microbiological profile information revealed that MAG changed the gut microbiota, especially by elevating the abundances of Lactobacillus, Faecalibacterium, and Butyricicoccus. Moreover, non-targeted metabolomic analysis showed that MAG significantly promoted tryptophan metabolites, which was positively correlated with the MAG-enriched gut microbiota. The increased tryptophan metabolites could activate aryl hydrocarbon receptor (AhR) and relieved hepatic inflammation and immune response evidenced by the downregulated the gene expression levels of pro-inflammatory cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in the liver. The fecal microbiota transplantation (FMT) experiments further confirmed that the hepatoprotective effect is likely mediated by MAG-altered gut microbiota and their metabolites.

Conclusions

Magnolol can be an outstanding supplement for the prevention and mitigation of FLHS in laying hens by positively regulating lipid synthesis and transport metabolism, improving the intestinal barrier function, and relieving hepatic inflammation by reshaping the gut microbiota and metabolite profiles through gut microbiota-indole metabolite-hepatic AhR crosstalk. These findings elucidate the mechanisms by which MAG alleviates FLHS and provide a promising method for preventing liver diseases by modulating gut microbiota and their metabolites.
Identifying ADGRG1 as a specific marker for tumor-reactive T cells in acute myeloid leukemia
Authors: Mei Yihan , Liu Yu , Liu Wenbing , Chen Manling , Liu Xiaoyu , Wang Shangshang , Mou Junli , Xing Haiyan , Tang Kejing , Tian Zheng , Rao Qing , Wang Min , Gu Runxia , Qiu Shaowei , Wang Jianxiang
Published: September 2024

Besides chemotherapy and hematopoietic stem cell transplantation (HSCT), autologous T cells can also serve as a new treatment approach for AML patients. However, the features of tumor-reactive T cells and their distinctive markers still lack full description. To evaluate the characteristics of tumor-reactive T cells, we collected bone marrow (BM) T cells from newly diagnosed AML patients with RUNX1::RUNX1T1 as examples for paired single-cell RNA sequencing and single-cell V(D)J sequencing. Based on the STARTRAC-like algorithm, we defined bystander T cells and tumor-reactive T cells. Compared with bystander T cells, tumor-reactive T cells presented as senescent-like cytotoxic terminally differentiated T cells (Temra) with upregulated NK-related markers. Additionally, we found ADGRG1 could serve as the specific marker of CD8+ T tumor-reactive T cell and validated it through the Runx1Runx1t1/+; Mx1-Cre mouse model. In chimeric antigen receptor (CAR)-T and target cell system, ADGRG1 was selectively upregulated upon antigen-TCR encounter. Moreover, ADGRG1+CD8+ T cells released a higher level of IFN-γ and showed higher cell-killing ability when exposed to matched AML blasts. Together, our findings depict the single-cell profile of tumor-reactive T cells in AML BM and propose that ADGRG1 can act as an indicator of T cell tumor reactivity in AML, which may be further harnessed for adoptive cell therapy and tumor-reactive TCR enrichment.
An L-type calcium channel blocker nimodipine exerts anti-fibrotic effects by attenuating TGF-β1 induced calcium response in an in vitro model of thyroid eye disease
Authors: Chen Qian , Pan Yuan , Hu Yunwei , Chen Guanyu , Chen Xiaoqing , Xie Yanyan , Wang Minzhen , Li Zhuang , Huang Jun , Shi Yuxun , Huang Haixiang , Zhang Te , Wang Mei , Zeng Peng , Wang Sha , Chen Rongxin , Zheng Yongxin , Zhong Liuxueying , Yang Huasheng , Liang Dan
Published: September 2024

Background

Thyroid eye disease (TED) is a vision-threatening autoimmune disorder. Orbital tissue fibrosis leading to intractable complications remains a troublesome issue in TED management. Exploration of novel therapeutic targets and agents to ameliorate tissue fibrosis is crucial for TED. Recent work suggests that Ca2+ signaling participates in tissue fibrosis. However, whether an alteration of Ca2+ signaling has a role in fibrogenesis during TED remains unclear. In this study, we aimed to investigate the role of Ca2+ signaling in the fibrogenesis process during TED and the potential therapeutic effects of a highly selective inhibitor of the L-type calcium channel (LTCC), nimodipine, through a TGF-β1 induced in vitro TED model.

Methods

Primary culture of orbital fibroblasts (OFs) were established from orbital adipose connective tissues of patients with TED and healthy control donors. Real-time quantitative polymerase chain reaction (RT-qPCR) and RNA sequencing were used to assess the genes expression associated with LTCC in OFs. Flow cytometry, RT-qPCR, 5-ethynyl-2′-deoxyuridine (EdU) proliferation assay, wound healing assay and Western blot (WB) were used to assess the intracellular Ca2+ response on TGF-β1 stimulation, and to evaluate the potential therapeutic effects of nimodipine in the TGF-β1 induced in vitro TED model. The roles of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and signal transducer and activator of transcription 1 (STAT1) in fibrogenesis during TED were determined by immunohistochemistry, WB, flow cytometry and co-immunoprecipitation assay. Selective inhibitors were used to explore the downstream signaling pathways.

Results

LTCC inhibitor nimodipine blocked the TGF-β1 induced intracellular Ca2+ response and further reduced the expression of alpha-smooth muscle actin (α-SMA), collagen type I alpha 1 (Col1A1) and collagen type I alpha 2 (Col1A2) in OFs. Besides, nimodipine inhibited cell proliferation and migration of OFs. Moreover, our results provided evidence that activation of the CaMKII/STAT1 signaling pathway was involved in fibrogenesis during TED, and nimodipine inhibited the pro-fibrotic functions of OFs by down-regulating the CaMKII/STAT1 signaling pathway.

Conclusions

TGF-β1 induces an LTCC-mediated Ca2+ response, followed by activation of CaMKII/STAT1 signaling pathway, which promotes the pro-fibrotic functions of OFs and participates in fibrogenesis during TED. Nimodipine exerts potent anti-fibrotic benefits in vitro by suppressing the CaMKII/STAT1 signaling pathway. Our work deepens our understanding of the fibrogenesis process during TED and provides potential therapeutic targets and alternative candidate for TED.
Exploring the molecular mechanism of ginseng against anthracycline-induced cardiotoxicity based on network pharmacology, molecular docking and molecular dynamics simulation
Authors: Xie Lin , Liu Hanze , Zhang Ke , Pan Yijun , Chen Mengyao , Xue Xiangyue , Wan Guoxing
Published: September 2024

Background

Previous clinical and basic studies have revealed that ginseng might have cardioprotective properties against anthracycline-induced cardiotoxicity (AIC). However, the underlying mechanism of ginseng action against AIC remains insufficiently understood. The aim of this study was to explore the related targets and pathways of ginseng against AIC using network pharmacology, molecular docking, cellular thermal shift assay (CETSA) and molecular dynamics (MD) simulations.

Results

Fourteen drug-disease common targets were identified. Enrichment analysis showed that the AGE-RAGE in diabetic complications, fluid shear stress and atherosclerosis, and TNF signaling pathway were potentially involved in the action of ginseng against AIC. Molecular docking demonstrated that the core components including Kaempferol, beta-Sitosterol, and Fumarine had notable binding activity with the three core targets CCNA2, STAT1, and ICAM1. Furthermore, the stable complex of STAT1 and Kaempferol with favorable affinity was further confirmed by CETSA and MD simulation.

Conclusions

This study suggested that ginseng might exert their protective effects against AIC through the derived effector compounds beta-Sitosterol, Kaempferol and Fumarine by targeting CCNA2, STAT1, and ICAM1, and modulating AGE-RAGE in diabetic complications, fluid shear stress and atherosclerosis, and TNF signaling pathways.
Does the distribution of Wormian bone frequencies across different world regions reflect genetic affinity between populations?
Authors: Movsesian Alla
Published: September 2024

Background

Wormian, or sutural bones, are additional, irregularly shaped bone fragments that can occur within cranial sutures. These bones may arise due to various factors, including mechanical pressure on skull bones during early ontogenetic stages, such as during artificial cranial deformations, or due to genetic and environmental influences. This study investigates the potential genetic basis of sutural bones by comparing their frequencies across diverse global regions. It analyzed 33 craniological series, encompassing 2059 crania, to assess the frequency of sutural bones in the coronal, squamous, lambdoid, and occipitomastoid sutures among skeletal populations from regions including Aboriginal Australia, Melanesia, Southeast Asia, Siberia, Europe, and Native America. Biological distances between populations were calculated using Smith’s mean measure of divergence (MMD), with results visualized through multidimensional scaling.

Results

The analysis identified distinct clusters of Caucasian and Siberian populations. Siberian aboriginal populations are compactly grouped, consistent with mtDNA data indicating genetic roots dating back to the Neolithic inhabitants of the Lake Baikal region. Further, differentiation within these populations is linked to the founder effect and gene flow. Notably, genetically related groups like the Inuit and Chukchi of Chukotka differ from other Siberian groups. In contrast, southern Siberian populations, such as the Buryats and Mongols, are closely positioned, aligning with genetic data. The differentiation between Southeast Asian and African regions was subtler, with their clusters largely overlapping. Yet, genetic links between populations were observed in some cases. Thus, Australians, Melanesians, and Papua New Guineans were located close to each other on the multidimensional scaling map, as were two African populations.

Conclusions

The findings tentatively suggest a potential genetic component in the expression of Wormian bones, although this hypothesis requires further empirical support, particularly through genetic studies. While genetic factors may influence the expression of Wormian bones, environmental conditions and pathological processes also play significant roles. It can be suggested that Wormian bones could potentially serve as an additional tool in kinship analysis within burials; however, their utility significantly depends on the extent of their genetic influence. If future genetic studies confirm a substantial genetic component and its dominance over environmental factors, the use of these bones in anthropological and forensic analyses would receive additional validation.
Artificial intelligence: transforming cardiovascular healthcare in Africa
Authors: Ashinze Patrick , Akande Eniola , Bethrand Chukwu , Obafemi Eniola , David Olafisoye-Oragbade Oluwatosin , Akobe Suleiman Nasiru , Joyce Ndubuisi Onyinyechukwu , Izuchukwu Obidiegwu Jonathan , Okoro Ngozi Peace
Published: September 2024

Background

Cardiovascular diseases (CVDs), a significant global health concern, are responsible for 13% of all deaths particularly in Africa, where they contribute substantially to the global disease burden, taking several millions of lives globally and annually. Despite advancements in healthcare, the burden of CVDs continues to rise steadily. This comprehensive review critically examines the intersection of artificial intelligence (AI) and cardiovascular disease (CVD) management in Africa. Drawing on a diverse gamut of scholarly literature and empirical evidence, the review assesses the prevalence, impact, and challenges of CVDs in the African context.

Main body

The review highlights the potential of AI technologies to revolutionize CVD care, offering insights into its applications in diagnosis, treatment optimization, and remote patient monitoring. It explores existing literature sourced from databases like PUBMED, Scopus and Google Scholar about the current state of AI implementation in African healthcare systems, which are majorly resource-constrained, discussing successes, limitations, and future prospects. The work includes the prevalence and impact of CVDs in Africa, noting the significant public health burden and economic implications. Current challenges in addressing CVDs are outlined, focusing on resource constraints, healthcare system challenges, and socioeconomic factors. Our review takes a dive into AI’s role in healthcare, emphasizing its capabilities in disease diagnosis, treatment optimization, and patient monitoring, and presents current applications and case studies of AI in African cardiovascular healthcare. It also addresses the challenges and limitations of implementing AI in this context, such as inadequate infrastructure, lack of high-quality data, and the need for regulatory frameworks.

Conclusion

Our review emphasizes the urgent need for collaborative efforts among policymakers, healthcare providers, and researchers to overcome barriers to AI integration and ensure equitable access to innovative healthcare solutions. By fetching existing research and offering practical recommendations, this review contributes to the academic discourse on AI-driven healthcare interventions in Africa, offering an understanding of the opportunities and challenges in leveraging technology to address pressing public health concerns. It calls for increased research, investment, and collaboration to harness AI’s full potential in transforming cardiovascular healthcare in Africa.
Skin cancer classification using non-local means denoising and sparse dictionary learning based CNN
Authors: Pandey Apeksha , Teja Manepalli Sai , Sahare Parul , Kamble Vipin , Parate Mayur , Hashmi Mohammad Farukh
Published: September 2024

Skin conditions are becoming increasingly prevalent across the world in current times. With the rise in dermatological disorders, there is a need for computerized techniques that are completely noninvasive to patients’ skin. As a result, deep learning models have become standard for the computerized detection of skin diseases. The performance efficiency of these models improves with access to more data with their primary aim being image classification. In this paper, we present a skin disease detection methodology using image processing techniques, non-local means denoising and convolutional neural network (CNN) backed by sparse dictionary learning. Here, the major benefit of using NLM denoising followed by sparse dictionary learning with CNNs in image classification lies in leveraging a multi-stage approach that enhances the quality of input data, extracts meaningful and discriminative features, and improves the overall performance of the classification model. This combined approach addresses challenges such as noise robustness, feature extraction, and classification accuracy, making it particularly effective in complex image analysis tasks. For denoising, the average Peak Signal to Noise Ratio (PSNR) obtained for images from HAM-10000 dataset is 33.59 dB. For the ISIC-2019 dataset, the average PSNR for the train folder is 34.37 dB, and for the test folder it is 34.39 dB. The deep learning network is trained for the analysis of skin cancer images using a CNN model and is achieving acceptable results in classifying skin cancer types. The datasets used contain high-resolution images. After all the tests, the accuracy obtained is 85.61% for the HAM-10000 dataset and 81.23% for the ISIC-2019 dataset, which is on par with existing approaches validated by benchmarking results.
When do shoppers prefer using QR codes? Empirical evidence from Vietnam
Authors: Nguyen Man The , Alang Tho
Published: September 2024

This research aimed to explore the factors influencing the behavioural intention to use over-the-counter (OTC) QR codes for purchasing products in Vietnamese supermarkets. The study included 996 consumers and employed a questionnaire-based survey method to test the hypotheses using partial least squares structural equation modelling (PLS-SEM). Drawing on the theory of reasoned action (TRA) and signalling theory, the findings identified five key factors that positively affected the intention to use OTC QR codes: perceived usefulness of OTC QR codes (e.g., for payment and traceability), attitude, customer satisfaction, and supply chain transparency. Additionally, the perceived usefulness of OTC QR codes positively impacted attitude and customer satisfaction, while its usefulness for traceability enhanced supply chain transparency. The study also identified the moderating influence of personal innovativeness on the relationship between perceived usefulness of OTC QR codes for payment and attitude and customer satisfaction. Furthermore, the moderating role of electronic Word-of-Mouth (e-Word-of-Mouth) in the relationship between customer satisfaction, attitude, and behavioural intention was discovered. This research contributes to the existing literature by expanding our understanding of the perceived usefulness of OTC QR codes and elucidating the various mechanisms through which their both functionalities could encourage consumer adoption in the context of Vietnamese supermarkets.
Monitoring the Efficacy of Auditory Training through Changes in Brainstem and Cortical Auditory Evoked Potentials: A Systematic Review
Authors: Hajimohammadi Ali , Heidari Fatemeh
Published: September 2024

Introduction

Auditory training (AT) exercises as the main part of auditory rehabilitation have emerged as a promising method for enhancing auditory perception and communication skills in individuals with hearing impairments or difficulties in auditory processing. Through AT, the central auditory nervous system (CANS) undergoes changes that optimize neural circuits, resulting in improved auditory perception. Auditory-evoked potentials (AEPs), including the auditory brainstem responses (ABRs) and cortical auditory-evoked potentials (CAEPs), offer objective measurements of neural responses and serve as valuable biomarkers to assess the effectiveness of AT.

Methods

For this systematic review, we conducted a comprehensive search in multiple databases, including MEDLINE (via PubMed), Science Direct, Web of Science, and SciELO, up until August 18, 2023. There were no study type restrictions or limitations on publication time. Following a careful assessment of the article quality and their alignment with the inclusion and exclusion criteria, a total of 25 articles were selected for inclusion in this study.

Results

Based on the findings of the reviewed studies, it has been reported that AT exercises lead to an increase in the amplitude of waves in both brainstem and cortical AEPs, with the exception of P1 and N2 waves. Furthermore, it has been observed that the latency of these responses decreases following AT.

Conclusion

The application of brainstem and cortical AEPs as objective electrophysiological tools holds promise in assessing the effectiveness of AT exercises and confirming the selected approach for AT.
Effect of Adding Electroacupuncture to Standard Triple Antiemetic Therapy on Chemotherapy-Induced Nausea and Vomiting: A Randomized Controlled Clinical Trial
Authors: Shen Guoshuang , Ren Dengfeng , Zhao Fuxing , Wang Miaozhou , Liu Zhen , Feng Xiumei , He Yingjian , Liu Xinlan , Ling Xiaoling , Zhao Yi , Song Feixue , Ma Jiyan , Li Enxi , She Tong , Liu Zhilin , Li Zitao , Huang Shifen , Chen Yongzhi , Yang Jingru , Li Xuan , Duan Li , Da Mengting , Zheng Yonghui , Li Yongxin , Zhao Jiuda
Published: September 2024

PURPOSE

We aim to determine the effectiveness of adding electroacupuncture to standard triple antiemetic therapy for treating chemotherapy-induced nausea and vomiting (CINV).

METHODS

From March 2022 to December 2023, a randomized, blind, sham-controlled trial conducted across six Chinese hospitals investigated patients with breast cancer undergoing highly emetogenic chemotherapy (HEC). Patients were randomly assigned to either true electroacupuncture (n = 120) or sham electroacupuncture (n = 119) groups, with both groups receiving standard triple antiemetic therapy. The primary end point was the proportion of complete protection (no vomiting, no need for rescue treatment, and no significant nausea, as evaluated using the visual analog scale [VAS]) within 120 hours after receiving HEC.

RESULTS

Among 239 randomly assigned patients, 235 (98.3%) completed the trial. In the full analysis set, compared with the sham electroacupuncture group, the true electroacupuncture group demonstrated a significant increase in the complete protection rate from 34.5% to 52.9% (
P
= .004). Additionally, true electroacupuncture also showed enhanced total control (4.3%
v
13.4%;
P
= .014), no significant nausea (37.9%
v
58.8%;
P
= .001), no nausea (4.3%
v
13.4%;
P
= .014), and nausea VAS score = 0 mm (4.3%
v
12.6%;
P
= .023). However, the occurrence of no vomiting in the overall stage was similar (76.7%
v
73.9%;
P
= .622) in both groups. Post hoc exploratory analysis showed a significantly higher rate of complete protection during the delayed stage in the true electroacupuncture group compared with the sham electroacupuncture group, with no significant difference observed during the acute stage.

CONCLUSION

Adding true electroacupuncture to standard triple antiemetic therapy significantly enhances the efficacy of CINV treatment in patients with breast cancer receiving HEC.
Sanctuary Everywhere
Authors: Sostaita Barbara Andrea
Published: September 2024

In Sanctuary Everywhere, Barbara Andrea Sostaita reimagines practices of sanctuary along the U.S.-Mexico border in order to explore the possibilities for radical fugitivity in the face of militarized border enforcement. After the 2016 presidential election, churches, universities, cities, and even states began declaring themselves sanctuaries. Sostaita proposes that these calls for expanded sanctuary are insufficient when dealing with the everyday workings of immigration enforcement. Through fieldwork in migrant clinics, shelters, and the Sonoran Desert, Sostaita demonstrates that, as a sacred practice, sanctuary cannot be fixed in any one destination or mandate. She turns to those working to create sanctuary on the move, from a deported nurse offering medical care on the border to incarcerated migrant women denying rules on touch in detention facilities to collectives set up to honor those who died crossing the border. Understanding sanctuary to be a set of fugitive practices that escapes the everyday, Sostaita shows us how, in the wake of extreme violence and loss, migrants create sanctuaries of their own to care for the living and the dead.
The Wealth of a Nation: Institutional Foundations of English Capitalism
Authors: Clark Gregory
Published: September 2024

Gregory Clark of University of Southern Denmark reviews “The Wealth of a Nation: Institutional Foundations of English Capitalism” by Geoffrey M. Hodgson. The Econlit abstract of this book begins: “Discusses the English economy from 1300 to about 1820, focusing on the foundational conditions that enabled a dramatic transition from stagnation to growth that occurred in the seventeenth and eighteenth centuries.”