bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Effects of Fundamental and Longitudinal Life History Trade-Offs on Delay Discounting: An Evolutionary Framework
Authors: Lu Junsong , Lu Qi’an , Lu Lin
Published: March 2022

We synthesized life history theory and the antagonistic pleiotropy hypothesis to form an integrative framework for understanding delay discounting (DD). We distinguished between fundamental and longitudinal life history trade-offs to explain individual and age differences of DD. Fundamental life history trade-offs are characterized by life history strategies (LHS), describing how individuals adjust reproductive timing according to childhood environments, while longitudinal life history trade-offs characterize how individuals make trade-offs between early- vs. late-life reproduction as a function of age. Results of a life-span sample (242 Chinese participants) supported several theoretical predictions: (a) slower LHS predicted lower DD; (b) the relationship between chronological age and DD was U-shaped; (c) the effects of age and LHS were differential. Mechanisms underlying fundamental and longitudinal trade-offs were explored. Regarding fundamental trade-offs, LHS mediated the effects of childhood environment on DD. Regarding longitudinal trade-offs, the U-shaped relationship was more evident between physical age and DD: older adults who were in poorer physical health felt older and exhibited a higher DD. Neither the time perspective nor anticipatory time perception mediated the effect of life history trade-offs. We concluded that DD was a product of two distinct life history trade-offs, reflecting both the trait-like quality and age-related development.
Peak and Cumulative Response of Reinforced Concrete Frames with Steel Damper Columns under Seismic Sequences
Authors: Fujii Kenji
Published: March 2022

The steel damper column is an energy-dissipating member that is suitable for reinforced concrete (RC) buildings and those used for multistory housing in particular. However, the effectiveness of steel damper columns may be affected by the behavior of surrounding members, and this effect can be severe in the case of seismic sequences. This article investigates the nonlinear response of building models with an RC moment-resisting frame (MRF) with and without steel damper columns under seismic sequences. The applicability of the concept of the momentary energy input to the prediction of the peak response of RC MRFs with damper columns under seismic sequences is also investigated. The main findings of the study are summarized as follows. (1) The peak response of RC MRFs with damper columns subjected to sequential accelerations is similar to the peak response obtained considering only the mainshock, whereas the cumulative strain energy of RC MRFs accumulates more for sequential accelerations. (2) The steel damper column is effective in reducing the peak and cumulative responses of RC MRFs in the case of sequential seismic input. (3) The relation of the hysteretic dissipated energy during a half cycle of the structural response and the peak displacement of the first modal response can be properly evaluated using the simple model proposed in this study.
Fiber-Type Influence on the Flexural Behavior of RC Two-Way Slabs with an Opening
Authors: Hussain Haleem , Abbas Abdulnasser , Ojaimi Mohammed
Published: March 2022

Combining fiber with concrete mixes has become essential and its widespread use improves the strength of structural concrete elements. This research conducted an experiment into the structural performance of flat slabs with and without a square opening using four types of fiber (hooked-end, straight, corrugated steel fiber and polyolefin fiber) to gain a better understanding of how the variance of fiber type and shape effects the flexural behaviors of two-way slabs. The test program involved (a) testing the properties of hardened concrete, such as compressive properties, modulus of rupture and splitting tensile strength, and (b) testing the flexural behavior of two-way slabs. Ten slabs were divided into five pairs, including two specimens used as reference specimens (with and without openings), and eight other specimens with different types of fibers. Results revealed that the existing fiber in concrete improved the mechanical properties of hardened concrete mix, and the compressive strength test showed higher improvement in specimens with hooked and straight steel fiber. The flexural behavior of reinforced concrete slab was significantly enhanced, and the flexural strength capacity was especially improved for the slabs strengthened with hooked-end and corrugated steel fiber. Polyolefin fiber showed a slight enhancement of mechanical properties and good improvement in flexural capacity. Generally, the highest increments in compressive strength and modulus of rupture were 24.8%, 20% and 11%, and the ultimate load-carrying capacity of slabs was 39%, 13% and 19% for specimens with steel hooked, steel corrugated and polyolefin fibers, respectively, compared with control specimens.
Information and Communication Technology (ICT) Utilization and Infrastructure Alignment in Construction Organizations
Authors: Eliwa Hassan Khames , Jelodar Mostafa Babaeian , Poshdar Mani
Published: March 2022

Information and communication technology (ICT) is one of the important factors that support construction project performance. Various studies have concluded that by aligning the ICT utilization with organizational ICT infrastructure, better performance outcomes can be achieved from the project to organization level. A positive connection between utilization of ICT and project performance has been established. However, the effects of ICT utilization strategy alignment with the organizational ICT infrastructure on construction project performance have not been investigated. This research evaluates and analyzes the importance of organizational ICT infrastructure to improve ICT utilization not just at the project level but also at the level of construction organizations. The main objective of this research is to investigate whether the effect of ICT utilization on project performance may be restricted and affected by its alignment with the ICT infrastructure level in the organization. The analyses of ICT infrastructure, ICT usage, and their associations with project performance are based on case studies of projects and organizations from the New Zealand construction sector. A method of evaluating ICT utilization and ICT infrastructure is proposed. This allows for further detailed examination of the strategic alignment between ICT utilization and organizational infrastructure. The effects of this strategic alignment on project performance are discussed and recommendations for the optimal strategic utilization are provided.
Hairy Cell Leukemia Patients Have a Normal Life Expectancy—A 35-Year Single-Center Experience and Comparison with the General Population
Authors: Bohn Jan-Paul , Neururer Sabrina , Pirklbauer Markus , Pircher Andreas , Wolf Dominik
Published: March 2022

Classic hairy cell leukemia (HCL) is an uncommon hematologic malignancy characterized by an excellent prognosis since purine analogues (PA), such as cladribine (2-CdA), have been introduced in the 1990s. However, most data on long-term outcomes is gathered from patients treated with PA first-line or include limited information on previous treatment outcomes, i.e., Interferon-α (IFN-α). Survival curves from previous series did not reach a plateau, indicating that nearly all patients ultimately relapse. Yet, overall survival (OS) data were rarely corrected for life expectancy of the general population. We here report 83 consecutive HCL patients treated between 1983 and 2017 at the University Center in Innsbruck, Austria. Median follow-up was 170 months (1–498). IFN-α, the first-line treatment of choice before 1990, was administered to 24 patients, achieving an overall response rate (ORR) of 86% and an unconfirmed complete remission (CRu) in 23%. All these patients relapsed after a median progression-free survival (PFS) of 30 months (3–80), but either remained drug-sensitive upon re-exposure to IFN-α or were successfully salvaged with PA. All 42 patients exposed to first-line 2-CdA responded (ORR of 100%). Sixteen patients received two to four successive courses of PA with a continuous decrease in the response quality (CRu rate 85.7% 1st-line vs. 41.5% 3rd-line treatment). Median PFS was not reached in both treatment-naïve patients and those retreated at first relapse. Although pretreatment with IFN-α was associated with a shortened median PFS of 81 months (43–118) after PA therapy, this tendency of inferior PFS did not result in inferior OS. OS of all 83 patients was excellent and equivalent to that of age-, sex-, and diagnostic period-matched controls from the Tyrolean general population (standardized mortality ratio 0.8), regardless of their age at diagnosis or whether they were diagnosed until or after the year 2000. These results confirm that HCL patients may look forward to a normal lifespan when treated with PA irrespective of their pretreatment history.
Impact of UGT1A1 Polymorphisms on Febrile Neutropenia in Pancreatic Cancer Patients Receiving FOLFIRINOX: A Single-Center Cohort Study
Authors: Keum Jiyoung , Lee Hee Seung , Jo Jung Hyun , Chung Moon Jae , Park Jeong Youp , Park Seung Woo , Song Si Young , Bang Seungmin
Published: March 2022

FOLFIRINOX (oxaliplatin, leucovorin, irinotecan, and 5-fluorouracil) is a first-line chemotherapy for metastatic pancreatic cancer (PC). Chemotherapy-induced neutropenia is one of the most serious adverse events associated with advanced PC. Although UGT1A1 polymorphisms are associated with the metabolism of irinotecan, their role as surrogate markers for FOLFIRINOX-induced neutropenia has not been confirmed. We investigated risk factors for FN—in particular, UGT1A1 polymorphisms—in PC patients receiving FOLFIRINOX, using a single-center cohort registry. To investigate the association between UGT1A1 polymorphisms and FN, we divided patients into three groups based on the predicted UGT1A1 phenotype: extensive metabolizer (EM) vs. intermediate metabolizer (IM) vs. poor metabolizer (PM). A total of 154 patients (FN group (n = 31) vs. non-FN group (n = 123)) receiving first-line FOLFIRINOX were identified between December 2017 and July 2020. The Cox regression analysis showed that female sex (HR: 2.20; p = 0.031), Eastern Cooperative Oncology Group performance status = 1 (HR: 2.83; p = 0.008), UGT1A1 IM (HR: 4.30; p = 0.004), and UGT1A1 PM (HR: 4.03; p = 0.028) were statistically significant risk factors for FN. We propose that UGT1A1 is the strongest predictive factor for FN and that this gene should be screened prior to the administration of chemotherapy.
Immune Checkpoint Inhibitors in Advanced Prostate Cancer: Current Data and Future Perspectives
Authors: Rebuzzi Sara Elena , Rescigno Pasquale , Catalano Fabio , Mollica Veronica , Vogl Ursula Maria , Marandino Laura , Massari Francesco , Pereira Mestre Ricardo , Zanardi Elisa , Signori Alessio , Buti Sebastiano , Bauckneht Matteo , Gillessen Silke , Banna Giuseppe Luigi , Fornarini Giuseppe
Published: March 2022

In the last 10 years, many new therapeutic options have been approved in advanced prostate cancer (PCa) patients, granting a more prolonged survival in patients with metastatic disease, which, nevertheless, remains incurable. The emphasis on immune checkpoint inhibitors (ICIs) has led to many trials in this setting, with disappointing results until now. Therefore, we discuss the immunobiology of PCa, presenting ongoing trials and the available clinical data, to understand if immunotherapy could represent a valid option in this disease, and which subset of patients may be more likely to benefit. Current evidence suggests that the tumor microenvironment needs a qualitative rather than quantitative evaluation, along with the genomic determinants of prostate tumor cells. The prognostic or predictive value of immunotherapy biomarkers, such as PD-L1, TMB, or dMMR/MSI-high, needs further evaluation in PCa. Monotherapy with immune checkpoint inhibitors (ICIs) has been modestly effective. In contrast, combined strategies with other standard treatments (hormonal agents, chemotherapy, PARP inhibitors, radium-223, and TKIs) have shown some results. Immunotherapy should be better investigated in biomarker-selected patients, particularly with specific pathway aberrations (e.g., AR-V7 variant, HRD, CDK12 inactivated tumors, MSI-high tumors). Lastly, we present new possible targets in PCa that could potentially modulate the tumor microenvironment and improve antitumor activity with ICIs.
Hemodynamic, Surgical and Oncological Outcomes of 40 Distal Pancreatectomies with Celiac and Left Gastric Arteries Resection (DP CAR) without Arterial Reconstructions and Preoperative Embolization
Authors: Egorov Viacheslav , Kim Pavel , Kharazov Alexander , Dzigasov Soslan , Popov Pavel , Rykova Sofia , Zelter Pavel , Demidova Anna , Kondratiev Eugeny , Grigorievsky Maxim , Sorokin Alexander
Published: March 2022

DPCAR’s short- and long-term outcomes are highly diverse, while the causes and prevention of ischemic complications are unclear. To assess oncological, surgical, and hemodynamic outcomes of 40 consecutive DPCARs for pancreatic (n37) and gastric tumors (n3) (2009–2021), retrospective analyses of mortality, morbidity, survival, and hemodynamic consequences after DPCAR were undertaken using case history data, IOUS, and pre- and postoperative CT measurements. In postoperative complications (42.5%), the pancreatic fistula was the most frequent event (27%), 90-day mortality was 7.5. With 27 months median follow-up, median overall (OS) and progression-free survival (PFS) for PDAC were 29 and 18 months, respectively; with 1-, 3-, and 5-years, the OS were 90, 60, and 28%, with an R0-resection rate of 92.5%. Liver and gastric ischemia developed in 0 and 5 (12.5%) cases. Comparison of clinical and vascular geometry data revealed fast adaptation of collateral circulation, insignificant changes in proper hepatic artery diameter, and high risk of ischemic gastropathy if the preoperative diameter of pancreaticoduodenal artery was <2 mm. DP CAR can be performed with acceptable morbidity and survival. OS and RFS in this super-selective cohort were compared to those for resectable cancer. The changes in the postoperative arterial geometry could explain the causes of ischemic complications and determine directions for their prevention.
Targeting HIF-2α in the Tumor Microenvironment: Redefining the Role of HIF-2α for Solid Cancer Therapy
Authors: Davis Leah , Recktenwald Matthias , Hutt Evan , Fuller Schuyler , Briggs Madison , Goel Arnav , Daringer Nichole
Published: March 2022

Inadequate oxygen supply, or hypoxia, is characteristic of the tumor microenvironment and correlates with poor prognosis and therapeutic resistance. Hypoxia leads to the activation of the hypoxia-inducible factor (HIF) signaling pathway and stabilization of the HIF-α subunit, driving tumor progression. The homologous alpha subunits, HIF-1α and HIF-2α, are responsible for mediating the transcription of a multitude of critical proteins that control proliferation, angiogenic signaling, metastasis, and other oncogenic factors, both differentially and sequentially regulating the hypoxic response. Post-translational modifications of HIF play a central role in its behavior as a mediator of transcription, as well as the temporal transition from HIF-1α to HIF-2α that occurs in response to chronic hypoxia. While it is evident that HIF-α is highly dynamic, HIF-2α remains vastly under-considered. HIF-2α can intensify the behaviors of the most aggressive tumors by adapting the cell to oxidative stress, thereby promoting metastasis, tissue remodeling, angiogenesis, and upregulating cancer stem cell factors. The structure, function, hypoxic response, spatiotemporal dynamics, and roles in the progression and persistence of cancer of this HIF-2α molecule and its EPAS1 gene are highlighted in this review, alongside a discussion of current therapeutics and future directions.
Multimodal Treatment of Nasopharyngeal Carcinoma in Children, Adolescents and Young Adults-Extended Follow-Up of the NPC-2003-GPOH Study Cohort and Patients of the Interim Cohort
Authors: Römer Tristan , Franzen Sabrina , Kravets Hanna , Farrag Ahmed , Makowska Anna , Christiansen Hans , Eble Michael J. , Timmermann Beate , Staatz Gundula , Mottaghy Felix M. , Bührlen Martina , Hagenah Ulrich , Puzik Alexander , Driever Pablo Hernáiz , Greiner Jeanette , Jorch Norbert , Tippelt Stephan , Schneider Dominik T. , Kropshofer Gabriele , Overbeck Tobias R. , Christiansen Holger , Brozou Triantafyllia , Escherich Gabriele , Becker Martina , Friesenbichler Waltraud , Feuchtinger Tobias , Puppe Wolfram , Heussen Nicole , Hilgers Ralf D. , Kontny Udo
Published: March 2022

Nasopharyngeal carcinoma (NPC) in children and young adults has been treated within two consecutive prospective trials in Germany, the NPC-91 and the NPC-2003 study of the German Society of Pediatric Oncology and Hematology (GPOH). In these studies, multimodal treatment with induction chemotherapy, followed by radio (chemo)therapy and interferon-beta maintenance, yielded promising survival rates even after adapting total radiation doses to tumor response. The outcome of 45 patients in the NPC-2003 study was reassessed after a median follow-up of 85 months. In addition, we analyzed 21 further patients after closure of the NPC-2003 study, recruited between 2011 and 2017, and treated as per the NPC-2003 study protocol. The EFS and OS of 66 patients with locoregionally advanced NPC were 93.6% and 96.7%, respectively, after a median follow-up of 73 months. Seven patients with CR after induction therapy received a reduced radiation dose of 54 Gy; none relapsed. In young patients with advanced locoregional NPC, excellent long-term survival rates can be achieved by multimodal treatment, including interferon-beta. Radiation doses may be reduced in patients with complete remission after induction chemotherapy and may limit radiogenic late effects.
The Biology of Ocular Adnexal Marginal Zone Lymphomas
Authors: Johansson Patricia , Eckstein Anja , Küppers Ralf
Published: March 2022

This review focuses on the biology of ocular adnexal marginal zone B-cell lymphomas of the mucosa-associated lymphatic tissue (MALT) (OAMZL) subtype. The ocular adnexa includes all structures and tissues within the orbit except for the eye bulb. In the region of the ocular adnexa, MALT lymphomas represent the most common subtype of lymphoma, accounting for around 8% of all non-Hodgkin lymphomas. These lymphomas are often preceded by inflammatory precursor lesions. Either autoantigens or infectious antigens may lead to disease development by functioning as continuous antigenic triggers. This triggering leads to a constitutive activation of the NF-κB signaling pathway. The role of antigenic stimulation in the pathogenesis of OAMZL is supported by the detection of somatic mutations (partially with further intraclonal diversity) in their rearranged immunoglobulin V genes; hence, their derivation from germinal-center-experienced B cells, by a restricted IGHV gene usage, and the validation of autoreactivity of the antibodies in selected cases. In the established lymphomas, NF-κB activity is further enforced by mutations in various genes regulating NF-κB activity (e.g., TNFAIP3, MYD88), as well as recurrent chromosomal translocations affecting NF-κB pathway components in a subset of cases. Further pathogenetic mechanisms include mutations in genes of the NOTCH pathway, and of epigenetic regulators. While gene expression and sequencing studies are available, the role of differential methylation of lymphoma cells, the role of micro-RNAs, and the contribution of the microenvironment remain largely unexplored.
HER2–CDH1 Interaction via Wnt/B-Catenin Is Associated with Patients’ Survival in HER2-Positive Metastatic Gastric Adenocarcinoma
Authors: De Re Valli , Alessandrini Lara , Brisotto Giulia , Caggiari Laura , De Zorzi Mariangela , Casarotto Mariateresa , Miolo Gianmaria , Puglisi Fabio , Garattini Silvio Ken , Lonardi Sara , Cannizzaro Renato , Canzonieri Vincenzo , Fassan Matteo , Steffan Agostino
Published: March 2022

Trastuzumab is a human epidermal growth factor receptor 2 (HER2) inhibitor used to treat HER2+ metastatic gastric cancer (mGC). The present study aims to investigate the relationship between CDH1 mRNA expression and HER2-positivity in mGC using a multiplexed gene expression profile in two series of gastric cancer (GC): Series 1 (n = 38): HER2+ and HER2- mGC; Series 2 (n = 36) HER2- GC with and without metastasis. To confirm the results, the same expression profiles were analyzed in 354 GC from The Cancer Genome Atlas (TCGA) dataset. The difference in gene expression connected HER2 overexpression with canonical wingless-type (Wnt)/β-catenin pathway and immunohistochemical (IHC) expression loss of E-cadherin (E-CAD). CDH1 mRNA expression was simultaneously associated with the rs16260-A variant and an increase in E-CAD expression. Differences in retinoic acid receptor alfa (RARA), RPL19 (coding for the 60S ribosomal L19 protein), catenin delta 1 (CTNND1), and epidermal growth factor (EGF) mRNA levels—all included in the Wnt/β-catenin pathway—were found associated with overall survival (OS). RARA, CTNND1, and EGF resulted in independent OS prognostic factors. EGF was confirmed as an independent factor along with TNM stage in HER2-overpressed mGC from TCGA collection. Our study highlighted factors involved in the WNT/β-catenin pathway that interconnected E-CAD with HER2 overexpression and patient survival.
Level II Oncoplastic Surgery as an Alternative Option to Mastectomy with Immediate Breast Reconstruction in the Neoadjuvant Setting: A Multidisciplinary Single Center Experience
Authors: Di Leone Alba , Franco Antonio , Terribile Daniela Andreina , Magno Stefano , Fabi Alessandra , Sanchez Alejandro Martin , D’Archi Sabatino , Scardina Lorenzo , Natale Maria , Mason Elena Jane , Murando Federica , Marazzi Fabio , Orlandi Armando , Paris Ida , Visconti Giuseppe , Palazzo Antonella , Masiello Valeria , Barone Adesi Liliana , Salgarello Marzia , Masetti Riccardo , Franceschini Gianluca
Published: March 2022

Oncoplastic surgery level II techniques (OPSII) are used in patients with operable breast cancer. There is no evidence regarding their safety and efficacy after neoadjuvant chemotherapy (NAC). The aim of this study was to compare the oncological and aesthetic outcomes of this technique compared with those observed in mastectomy with immediate breast reconstruction (MIBR), in post-NAC patients undergoing surgery between January 2016 and March 2021. Local disease-free survival (L-DFS), regional disease-free survival (R-DFS), distant disease-free survival (D-DFS), and overall survival (OS) were compared; the aesthetic results and quality of life (QoL) were evaluated using BREAST-Q. A total of 297 patients were included, 87 of whom underwent OPSII and 210 of whom underwent MIBR. After a median follow-up of 39.5 months, local recurrence had occurred in 3 patients in the OPSII group (3.4%), and in 13 patients in the MIBR group (6.1%) (p = 0.408). The three-year L-DFS rates were 95.1% for OPSII and 96.2% for MIBR (p = 0.286). The three-year R-DFS rates were 100% and 96.4%, respectively (p = 0.559). The three-year D-DFS rate were 90.7% and 89.7% (p = 0.849). The three-year OS rates were 95.7% and 95% (p = 0.394). BREAST-Q highlighted significant advantages in physical well-being for OPSII. No difference was shown for satisfaction with breasts (p = 0.656) or psychosocial well-being (p = 0.444). OPSII is safe and effective after NAC. It allows oncological and aesthetic outcomes with a high QoL, and is a safe alternative for locally advanced tumors which are partial responders to NAC.
Detection of Circulating and Disseminated Tumor Cells and Their Prognostic Value under the Influence of Neoadjuvant Therapy in Esophageal Cancer Patients
Authors: Richter Florian , Röder Christian , Möller Thorben , Egberts Jan-Hendrik , Becker Thomas , Sebens Susanne
Published: March 2022

Detection of circulating (CTC) or disseminated tumor cells (DTC) are correlated with negative prognosis in esophageal cancer (EC) patients. In this study, DTC- and CTC-associated markers CK20 and DEFA5 were determined by RT-PCR in EC patients and correlated with clinical parameters to determine their prognostic impact. The blood and bone marrow (BM) of 216 EC patients after tumor resection with or without neoadjuvant therapy and as control blood samples from 38 healthy donors and BM from 24 patients with non-malignant diseases were analyzed. Both markers were detected in blood and BM of EC patients and the control cohort. A cut-off value was determined to define marker positivity for correlation with clinical data. CK20 expression was detected in 47/206 blood samples and in 49/147 BM samples of EC patients. DEFA5 positivity was determined in 96/206 blood samples and 98/147 BM samples, not correlating with overall survival (OS). However, CK20 positivity in BM and DEFA5 negativity in blood were associated with reduced OS in EC patients without neoadjuvant therapy, while in patients with neoadjuvant therapy DEFA5 positivity in BM was associated with improved OS. Overall, our study suggests DEFA5 as a prognostic biomarker in liquid biopsies of EC patients which requires further validation.
ZnO/ZnS-Polyvinyl Alcohol Hydrogel for Photocatalytic H2-Generation
Authors: Poliukhova Valeriia , Lei Wenwei , Khan Sovann , Tae Eunju Lee , Suzuki Norihiro , Terashima Chiaki , Fujishima Akira , Katsumata Ken-Ichi , Cho So-Hye
Published: March 2022

The separation of nanoparticles from a solution-based photocatalytic reaction is a significant problem in practical applications. To address the issue, we developed a new photocatalyst composite based on ZnO-ZnS heterojunction (ZnOS) embedded in polyvinyl alcohol (PVA) hydrogel, which showed satisfactory results for photocatalyst recycling. PVA-ZnOS composite hydrogel was fabricated by freezing-induced gelation, which enabled the encapsulation of ZnOS nanoparticles into polymeric matrices. PVA hydrogel served as a promising candidate in photocatalytic applications due to its excellent properties such as high transparency, porosity, hydrophilicity, and stability under ultraviolet (UV) light. PVA-ZnOS hydrogel showed worthy activity in H2 generation from Na2S/Na2SO3 aqueous solution under UV radiation with a production rate of 18.8 µmol·h−1. PVA-ZnOS composite hydrogel is a separation-free photocatalyst, which is prospective in a solution-based photocatalytic reactor.
Schwann Cells in Digestive System Disorders
Authors: Goluba Karina , Kunrade Liga , Riekstina Una , Parfejevs Vadims
Published: March 2022

Proper functioning of the digestive system is ensured by coordinated action of the central and peripheral nervous systems (PNS). Peripheral innervation of the digestive system can be viewed as intrinsic and extrinsic. The intrinsic portion is mainly composed of the neurons and glia of the enteric nervous system (ENS), while the extrinsic part is formed by sympathetic, parasympathetic, and sensory branches of the PNS. Glial cells are a crucial component of digestive tract innervation, and a great deal of research evidence highlights the important status of ENS glia in health and disease. In this review, we shift the focus a bit and discuss the functions of Schwann cells (SCs), the glial cells of the extrinsic innervation of the digestive system. For more context, we also provide information on the basic findings regarding the function of innervation in disorders of the digestive organs. We find diverse SC roles described particularly in the mouth, the pancreas, and the intestine. We note that most of the scientific evidence concerns the involvement of SCs in cancer progression and pain, but some research identifies stem cell functions and potential for regenerative medicine.
Molecular Mechanism of Lipotoxicity as an Interesting Aspect in the Development of Pathological States—Current View of Knowledge
Authors: Lipke Katarzyna , Kubis-Kubiak Adriana , Piwowar Agnieszka
Published: March 2022

Free fatty acids (FFAs) play numerous vital roles in the organism, such as contribution to energy generation and reserve, serving as an essential component of the cell membrane, or as ligands for nuclear receptors. However, the disturbance in fatty acid homeostasis, such as inefficient metabolism or intensified release from the site of storage, may result in increased serum FFA levels and eventually result in ectopic fat deposition, which is unfavorable for the organism. The cells are adjusted for the accumulation of FFA to a limited extent and so prolonged exposure to elevated FFA levels results in deleterious effects referred to as lipotoxicity. Lipotoxicity contributes to the development of diseases such as insulin resistance, diabetes, cardiovascular diseases, metabolic syndrome, and inflammation. The nonobvious organs recognized as the main lipotoxic goal of action are the pancreas, liver, skeletal muscles, cardiac muscle, and kidneys. However, lipotoxic effects to a significant extent are not organ-specific but affect fundamental cellular processes occurring in most cells. Therefore, the wider perception of cellular lipotoxic mechanisms and their interrelation may be beneficial for a better understanding of various diseases’ pathogenesis and seeking new pharmacological treatment approaches.
Transcriptional and DNA Methylation Signatures of Subcutaneous Adipose Tissue and Adipose-Derived Stem Cells in PCOS Women
Authors: Divoux Adeline , Erdos Edina , Whytock Katie , Osborne Timothy F. , Smith Steven R.
Published: March 2022

Polycystic ovary syndrome (PCOS) is often associated with metabolic syndrome features, including central obesity, suggesting that adipose tissue (AT) is a key organ in PCOS pathobiology. In this study, we compared both abdominal (ABD) and gluteofemoral (GF) subcutaneous AT in women with and without PCOS. ABD and GF subcutaneous ATs from PCOS and BMI/WHR-matched control women were analyzed by RT-qPCR, FACS and histology. ABD and GF adipose-derived stem cell (ASC) transcriptome and methylome were analyzed by RNA-seq and DNA methylation array. Similar to the control group with abdominal obesity, the GF AT of PCOS women showed lower expression of genes involved in lipid accumulation and angiogenesis compared to ABD depot. FACS analysis revealed an increase in preadipocytes number in both AT depots from PCOS. Further pathway analysis of RNA-seq comparisons demonstrated that the ASCs derived from PCOS are pro-inflammatory and exhibit a hypoxic signature in the ABD depot and have lower expression of adipogenic genes in GF depot. We also found a higher CpG methylation level in PCOS compared to control exclusively in GF-ASCs. Our data suggest that ASCs play an important role in the etiology of PCOS, potentially by limiting expansion of the healthy lower-body AT.
Silenced Coffee Rooms—The Changes in Social Capital within Social Workers’ Work Communities during the First Wave of the COVID-19 Pandemic
Authors: Saraniemi Sanna , Harrikari Timo , Fiorentino Vera , Romakkaniemi Marjo , Tiitinen Laura
Published: March 2022

The sudden outbreak of the COVID-19 pandemic and the ensuing restrictive measures to combat infections led to a significant change in working life and social work within working communities. Workers had to switch to telecommuting quickly, which also affected the interactions between co-workers. In this research, we examined Finnish social workers’ experiences of their work communities during the first wave of the COVID-19 pandemic. We explored (1) how the restrictive measures affected social workers’ work communities and (2) what types of factors promoted and challenged the cohesion of social networks and mutual trust between colleagues. The conceptual framework was based on social capital theory, in which social relations are seen as a resource of a community. The data utilised in the study were social workers’ diaries (n = 33) written from mid-March until the end of May 2020. The data were analysed by a qualitative content analysis. The results highlight how the multilocation of work, fear of viral infection and varying attitudes towards the viral outbreak affected the interactions between colleagues in the early stages of the pandemic, increasing tensions and feelings of social distance between co-workers. The common professional value and knowledge base of social work, as well as remote work practices developed during the first wave of the COVID-19 pandemic, supported interactions between colleagues. Although remote interaction options were developed, they could not, however, fully replace the advantages of face-to-face interactions and everyday informal encounters between colleagues, the importance of which is essential for developing and maintaining the social capital of work communities.
An Electrochemical and Raman Scattering Dual Detection Biosensor for Rapid Screening and Biomolecular Profiling of Cancer Biomarkers
Authors: Dey Shuvashis , Ahmed Emtiaz , Somvanshi Pranjal Satishchandra , Sina Abu Ali Ibn , Wuethrich Alain , Trau Matt
Published: March 2022

Detecting circulating biomarkers sensitively and quantitatively is paramount for cancer screening, diagnosis, and treatment selection. Particularly, screening of a panel of circulating protein biomarkers followed by mapping of individual biomarkers could assist better diagnosis and understanding of the cancer progression mechanisms. Herein, we present a miniaturized biosensing platform with dual readout schemes (electrochemical and Surface enhanced Raman scattering (SERS)) for rapid cancer screening and specific biomarker expressional profiling to support cancer management. Our approach utilizes a controlled nanomixing phenomena under alternative current electrohydrodynamic condition to improve the isolation of cancer-associated circulating proteins (i.e., Epidermal growth factor receptor (EGFR), BRAF, Programmed death-ligand 1 (PD-L1)) with antibody functionalized sensor surface for rapid and efficient isolation of the targets and subsequent labelling with SERS nanotags. The method employs Differential Pulse Voltammetry (DPV) for rapidly screening for the presence of the circulating proteins on biosensor surface irrespective of their type. Upon positive DPV detection, SERS is applied for sensitive read-out of individual biomarkers biomarker levels. In a proof-of-concept study, we demonstrate the dual detection biosensor for analysing circulating BRAF, EGFR and PDL-1 proteins and successfully screened both ensemble and individual biomarker expressional levels as low as 10 pg (1 ng/mL). Our findings clearly indicate the potential of the proposed method for cancer biomarker analysis which may drive the translation of this dual sensing concept in clinical settings.
Porous Pb-Doped ZnO Nanobelts with Enriched Oxygen Vacancies: Preparation and Their Chemiresistive Sensing Performance
Authors: Zheng Kai-Ge , Yang Tian-Yu , Guo Zheng
Published: March 2022

Among various approaches to improve the sensing performance of metal oxide, the metal-doped method is perceived as effective, and has received great attention and is widely investigated. However, it is still a challenge to construct heterogeneous metal-doped metal oxide with an excellent sensing performance. In the present study, porous Pb-doped ZnO nanobelts were prepared by a simply partial cation exchange method, followed by in situ thermal oxidation. Detailed characterization confirmed that Pb was uniformly distributed on porous nanobelts. Additionally, it occupied the Zn situation, not forming its oxides. The gas-sensing measurements revealed that 0.61 at% Pb-doped ZnO porous nanobelts exhibited a selectively enhanced response with long-term stability toward n-butanol among the investigated VOCs. The relative response to 50 ppm of n-butanol was up to 47.7 at the working temperature of 300 °C. Additionally, the response time was short (about 5 s). These results were mainly ascribed to the porous nanostructure, two-dimensional belt-like morphology, enriched oxygen vacancies and the specific synergistic effect from the Pb dopant. Finally, a possible sensing mechanism of porous Pb-doped ZnO nanobelts is proposed and discussed.
“It Is a Whole Different Life from the Life I Used to Live”: Assessing Parents’ Support Needs in Paediatric Palliative Care
Authors: Aoun Samar , Stegmann Roswitha , Deleuil Renee , Momber Suzanne , Cuddeford Lisa , Phillips Marianne , Lyon Maureen , Gill Fenella
Published: March 2022

Aims: This feasibility study aimed to systematically identify and address the support needs of parents of children with life-limiting illnesses and to assess whether the systematic approach was acceptable and relevant to parents. Methods: The CSNAT (Paediatric) intervention consisted of two assessment visits with the paediatric palliative care team, 2–8 weeks apart, comprising conversations about sources for support in a tertiary children hospital in Western Australia (2018–2019). Audio-recorded telephone interviews were conducted with parents, and inductive thematic analysis was undertaken. Results: All 28 parents who were involved in the intervention agreed to be interviewed. Five themes summarised their experience: caregiving challenges, perceived gaps and feelings of isolation; the usefulness and practicality of the systematic assessment; emotional responses to self-reflection; feelings of validation and empowerment; and received supports responsive to their needs. Conclusions: Parents appreciated the value of this systematic approach in engaging them in conversations about their needs and solutions to address them. While clinical service support was affirmed by parents, they were left wanting in other areas of practical, psychosocial, and emotional support. Palliative care services need to build stronger partnerships with supportive community networks through compassionate communities volunteer models of care to address the non-clinical needs of these families.
Leveraging Telemedicine to Spread Expertise in Neonatal Resuscitation
Authors: Pinheiro Joaquim M. B.
Published: March 2022

The development and potential applications of telemedicine in neonatal resuscitation were reviewed by Donohue and colleagues in 2019, in a manuscript that compiled seminal references in the field [...]
The Relation between Vitamin D Level and Lung Clearance Index in Cystic Fibrosis—A Pilot Study
Authors: Dediu Mihaela , Ciuca Ioana Mihaiela , Pop Liviu Laurentiu , Iacob Daniela
Published: March 2022

Background: Life expectancy has increased in cystic fibrosis (CF) patients; however, the rate of mortality is still high, and in a majority of cases, the cause of death is due to respiratory deterioration. Vitamin D plays an important role in immunity and infection prophylaxis, as its deficiency is associated with frequent infections. In CF patients, a deficit of liposoluble vitamins is common, despite daily supplementation. The aim of this study is to evaluate the relation between vitamin D status and lung function expressed by lung clearance index (LCI) in patients with CF. We also assessed the relation of factors such as nutritional status, genotype, and associated comorbidities such as Pseudomonas infection, cystic fibrosis-related diabetes (CFRD), and cystic fibrosis liver disease (CFLD) with vitamin D and LCI. Methods: A cross-sectional study was conducted at the National Cystic Fibrosis Center by analyzing patients with CF who presented in our center between November 2017 and November 2019. We enrolled in the study patients diagnosed with CF, who were followed up in our CF center and who were able to perform lung function tests. Patients in exacerbation were excluded. Results: A strong negative correlation was found between vitamin D and LCI (r = −0.69, p = 0.000). A lower vitamin D storage was found in patients with CFLD and CFRD. Higher LCI values were found among patients with chronic Pseudomonas infection, with BMI under the 25th percentile, or with associated CFLD. Conclusion: In CF patients, vitamin D plays an important role, and its deficit correlates with an impaired LCI. Vitamin D deficit is a risk factor in patients with associated comorbidities such as CFLD and CFRD. Chronic infection with Pseudomonas, the presence of impaired nutritional status, and CFLD are associated with a prolonged LCI.
Discordance between Clinical and Ultrasound Examinations in Juvenile Idiopathic Arthritis: An Experimental Approach
Authors: Licciardi Francesco , Petraz Marco , Covizzi Carlotta , Santarelli Francesca , Cirone Carlotta , Mulatero Roberta , Robasto Francesca , Dellepiane Marta , Martino Silvana , Montin Davide , Ravagnani Viviana
Published: March 2022

Clinical examination (CE) and musculoskeletal ultrasound (MSUS) of ten joints (knee, ankle, wrist, elbow, II-MCP) and their extra-articular (EA) compartments (tendons and bursae) were performed on 35 consecutive patients with active juvenile idiopathic arthritis (JIA) (active group) to test how the extension of MSUS examinations to EA changes the concordance between MSUS and CE. The overall concordance between CE and MSUS, measured with Cohen’s Kappa (k), was moderate (k = 0.43); the addition of EA MSUS increased the concordance in all joints, with the exclusion of II-MCP (k = 0.49). In the ankle and wrist, the k increase was relevant (k from 0.13 to 0.27 and 0.11 to 0.41). In the active group patients, we observed 44 subclinical synovitis; the number of subclinical synovitis per patient was correlated with JADAS-27 (p = 0.03) and was higher in a control group composed of 15 patients with persistent disease remission (1.3 vs. 0.4 p = 0.03). Our results show that EA compartments should always be evaluated during MSUS. Furthermore, we demonstrate a moderate concordance between CE and MSUS in JIA; the finding of subclinical synovitis is common in patients with active diseases and is related to disease activity.
Hydrology across Disciplines: Organization and Application Experiences of a Public Hydrological Service in Italy
Authors: Allodi Alessandro , Angelo Letizia , Bordini Fabio , Branchi Monica , Comune Elisa , Del Longo Mauro , Nicolosi Giuseppe , Noberini Mauro , Pizzera Filippo , Pugliese Alessio , Ricciardi Giuseppe , Tonelli Fabrizio , Tugnoli Franca , Zenoni Enrica
Published: March 2022

Water is a fundamental resource for human life and nature; flood management, water supply systems and water protection policies are a few examples of equally important disciplines across the whole hydrological cycle. The present work focuses on the creation and sharing of hydrological knowledge within public activities, with regard to materials and methods adopted for developing and supplying hydrological information, suitable to different stakeholders needs, throughout different disciplines and sectors of environment, economy, society, as well as research and analysis. The aim of this work is to better understand the market in order to increase the value of hydrological data, products and services, and to reduce potential gaps and overlapping areas. The method we developed is based on the example of the Hydrological Service of Emilia-Romagna Region, Italy. Institutional, legal and territorial frameworks as well as agency organization, materials, methods, instruments, activities, products and results are briefly described, focusing on those supporting civil and environmental protection, water management, infrastructure design, climate change adaptation and mitigation measures. We discuss the role of a public Administration in interdisciplinary activities, the links between the general background (e.g., territory, society, rules), organizations, actors, resources, tools, processes and results, by highlighting, where possible, a potential starting point for future research studies. Finally, this paper adopts a novel linguistic style, based on an informal format, in order to explore the set-up and follow-up of the Hydrological Service’s initiatives, with the final aim of sparking curiosity and building awareness, from different sectors and disciplines, which, ultimately, may benefit from the presented approaches.
Spoon-like Beams Generated with Exponential Phases
Authors: Guo Lei , Xia Tian , Xu Yiping , Xiong Yan , Leng Xian , Tao Shaohua , Tian Yonghong , Cheng Shubo
Published: March 2022

In this paper, we report a new kind of beam, named “spoon-like” beams, generated with the exponential phase. The intensity distributions and transverse energy flow of the spoon-like beam at the focal plane are analyzed theoretically and experimentally. The results demonstrate that the size of the spoon-like beam becomes enlarged with the increasing power exponent n, and the length of the spoon-like intensity trajectory becomes shorter with the increasing parameter p. Furthermore, there is an intensity gradient along the spoon-like trajectory of the beam, which introduces the intensity-gradient force exerted onto microparticles. The experiment on optical tweezers demonstrates that the focused beams can create spoon-like traps for the two-dimensional manipulation of particles.
Performance Evaluation and Thermal Shock Behavior of PS-PVD (Gd0.9Yb0.1)2Zr2O7/YSZ Thermal Barrier Coatings
Authors: Zhao Hongxu , Zhang Xiaofeng , Deng Chunming , Deng Ziqian , Chen Xiaolong
Published: March 2022

In this study, (Gd0.9Yb0.1)2Zr2O7 (GYbZ)/yttria-stabilized zirconia (YSZ) double-ceramic-layer (DCL) thermal barrier coatings (TBCs) were prepared by plasma spray-physical vapor deposition (PS-PVD). The microstructure, mechanical performance, and thermal shock behavior of coatings prepared with spraying distances of 600, 800, and 1000 mm were investigated. The GYbZ coating prepared with a spraying distance of 600 mm showed a closely packed columnar structure. However, the GYbZ coatings prepared with spraying distances of 800 and 1000 mm showed a quasi-columnar structure. The GYbZ coating prepared with a spraying distance of 800 mm had the thickest columnar crystals with obvious inter-columnar gaps. In addition, this coating exhibited excellent mechanical performance and the best thermal shock resistance. The primary failure patterns appearing during thermal shocking on the surface of TBCs can be classified into the following five types: caves, exfoliation, delamination cracks, spalled areas, and radiate cracks. Furthermore, the failure behavior of these coatings in water-quenching tests is clarified.
Explainable Artificial Intelligence Approach for the Early Prediction of Ventilator Support and Mortality in COVID-19 Patients
Authors: Aslam Nida
Published: March 2022

Early prediction of mortality and risk of deterioration in COVID-19 patients can reduce mortality and increase the opportunity for better and more timely treatment. In the current study, the DL model and explainable artificial intelligence (EAI) were combined to identify the impact of certain attributes on the prediction of mortality and ventilatory support in COVID-19 patients. Nevertheless, the DL model does not suffer from the curse of dimensionality, but in order to identify significant attributes, the EAI feature importance method was used. The DL model produced significant results; however, it lacks interpretability. The study was performed using COVID-19-hospitalized patients in King Abdulaziz Medical City, Riyadh. The dataset contains the patients’ demographic information, laboratory investigations, and chest X-ray (CXR) findings. The dataset used suffers from an imbalance; therefore, balanced accuracy, sensitivity, specificity, Youden index, and AUC measures were used to investigate the effectiveness of the proposed model. Furthermore, the experiments were conducted using original and SMOTE (over and under sampled) datasets. The proposed model outperforms the baseline study, with a balanced accuracy of 0.98 and an AUC of 0.998 for predicting mortality using the full-feature set. Meanwhile, for predicting ventilator support a highest balanced accuracy of 0.979 and an AUC of 0.981 was achieved. The proposed explainable prediction model will assist doctors in the early prediction of COVID-19 patients that are at risk of mortality or ventilatory support and improve the management of hospital resources.