bluuebunny/crossref_metadata_2025 huggingface dataset:
Barriers to health care access and utilization among aged indigents under the Livelihood Empowerment Against Poverty Programme (LEAP): the perspective of users and service providers in north-western Ghana
Authors: Domapielle Maximillian Kolbe , Dassah Cornelius , Dordaa Felix , Cheabu Benjamin Spears Ngmekpele , Sulemana Mohammed
Published: July 2023

Aim:

This article draws on the poverty and access to health care framework to explore the barriers to access and utilization of primary health care among aged indigents under the Livelihood Empowerment Against Poverty Programme (LEAP) in Ghana.

Background:

Although many developing countries have made progress in extending primary health care to their populations following the Alma-Ata Declaration of 1978, the establishment of the Millennium Development Goals, and the Sustainable Development Goals (SDGs), barriers remain pervasive, particularly among vulnerable population groups. Previous studies have hardly paid in-depth attention to this important indicator for measuring progress toward achieving SDG 3.

Methodology:

To this end, we conducted a case study of access to health care services and utilization among aged indigents enrolled on the LEAP programme in the Daffiama Bussie Issa District of the Upper West. We collected and analyzed qualitative data from indigents aged 65 years and above, health care providers, and staff of the LEAP and the National Health Insurance Scheme (NHIS).

Findings:

Our analysis found geographic inaccessibility of health care, high costs of drugs and related services, exclusion of essential services from NHIS benefits package, and irregular transfer of cash to negatively influence access and utilization of health care among aged LEAP beneficiaries in the district. In addition to the need to strengthen the economy, provide health infrastructure and human resources for health in rural areas, the government needs to review the beneficiaries’ bimonthly stipends to reflect the daily minimum wage, eliminate the delay in payments, and review the benefits package of the NHIS to include essential services and medical devices commonly used by aged people. Yet implementing these recommendations has affordability implications that require innovation to mobilize additional resources and create the desired fiscal space and institutions that can sustainably implement universal coverage programmes such as the LEAP.
α-Tubulin mutation Thr-239-Ile in annual bluegrass (Poa annua) induces variable responses to prodiamine and dithiopyr
Authors: Russell Eli C. , Rutland Claudia Ann , Patel Jinesh , Hall Nathan D. , Bi Bo , Li Xiao , McElroy J. Scott
Published: July 2023

Mitotic-inhibiting herbicides, like prodiamine and dithiopyr, are used to control annual bluegrass (Poa annua L.) preemergence in managed turfgrass; however, resistance to mitotic-inhibiting herbicides has evolved due to repeated applications of herbicide from a single mechanism of action. Three suspected resistant populations (R1, R2, and R3) were collected in Alabama and Florida and screened for resistance to prodiamine. Part of the α-tubulin gene was sequenced for known target-site mutations. Target-site mutations were reported in all three R populations, with each containing an amino acid substitution at position 239 from threonine to isoleucine (Thr-239-Ile). Previous research has indicated that the Thr-239-Ile mutation confers resistance to dinitroaniline herbicides in other species. Dose–response screens using prodiamine and dithiopyr were conducted and I50 values were calculated for R1, R2, and R3 using regression models based on seedling emergence. For prodiamine, I50 values for R1, R2, and R3 were 35.3, 502.7, and 91.5 g ai ha−1, respectively, resulting in 2.9-, 41.9-, and 7.6-fold resistance, respectively, when compared with a susceptible (S) population. For dithiopyr, I50 values for R1, R2, and R3 were 154.0, 114.2, and 190.1 g ai ha−1, respectively, resulting in 3.6-, 2.7-, and 4.5-fold resistance, respectively, when compared with an S population. When comparing I90 values with the highest labeled use rates, R2 had a 2.9-fold level of resistance to prodiamine, and R1, R2, and R3 had a 2.4-, 2.0-, and 3.2-fold levels of resistance to dithiopyr, respectively. This is the first report of a variable response in P. annua to prodiamine despite each R population possessing the same mutation.
Randomized Controlled Trial of an Online Suicide Prevention Gatekeeper Training Program
Authors: Hill Ryan M. , Picou Paige , Hussain Zain , Vieyra Blake A. , Perkins Kaylee M.
Published: July 2023

Abstract: Background: Suicide prevention gatekeeper training programs offer a unique opportunity to increase knowledge about suicide prevention, enhance risk identification, and reduce suicide-related stigma. Aims: This study evaluated the efficacy of an online suicide prevention gatekeeper training program in a randomized trial. Method: Participants were US college students ( N = 388). Participants (69.3% women) had a mean age of 19.01 years and identified primarily as Hispanic/Latinx (52.1%) and non-Hispanic White (22.4%). Participants completed pre- and posttest surveys and were randomly assigned to either the ASK About Suicide to Save a Life (AS + K?) suicide prevention gatekeeper training program or an information-only comparison intervention. Results: Participants in the AS + K? condition reported significantly greater gatekeeper preparedness and self-efficacy, and lower stigmatized attitudes at posttraining, as compared with those in the comparison condition. There were no significant differences in likelihood of using gatekeeper skills or suicide-related knowledge across groups. Limitations: This short-term study was not able to assess behavioral change resulting in use of gatekeeper skills over time. Conclusions: Completion of gatekeeper training resulted in increases in preparedness and self-efficacy for engaging in gatekeeper behaviors, as well as a reduction in suicide-related stigma. Despite small intervention effects, the online AS + K? training appears to be a promising program.
Anthropogenic Climate Change Negatively Impacts Vegetation and Forage Conditions in the Greater Four Corners Region
Authors: Williams Emily L. , Funk Chris , Shukla Shraddhanand
Published: July 2023

The Southwestern U.S. has been in a megadrought for the past two decades, stressing vegetation across the region and leading to poor forage conditions on rangelands. How has human‐induced climate change affected air temperatures and atmospheric evaporative demand in the greater Four Corners region of the Southwest, and what have the corresponding impacts been to vegetative productivity? We address this question via a multi‐step climate change detection and attribution analysis. We first relate evaporative demand (quantified by Vapor Pressure Deficit, or VPD) and precipitation to the Normalized Difference Vegetation Index (NDVI) in six unique plant‐precipitation zones. VPD is strongly negatively related to NDVI, and the strength of this relation is similar in magnitude to precipitation. We then use a large ensemble of climate change simulations to estimate the magnitude of VPD increases attributable to human‐induced climate change. We find that modest increases in air temperatures have led to large increases in VPD, with the influence of climate change about twice the inter‐annual standard deviation by 2020. Finally, we estimate the reductions in NDVI due to increased VPD and present the reductions in terms of total annual Aboveground Net Primary Productivity. We conclude that human‐induced warming has roughly doubled the magnitude of recent vegetation deficits, especially in mid‐precipitation areas (receiving 250–500 mm of precipitation annually), concentrated in New Mexico. These results indicate that increased temperatures from human‐caused climate change are having persistent and damaging impacts on vegetation productivity, with significant implications for ranchers and other land users in the region.
Non‐Monotonic Feedback Dependence Under Abrupt CO2 Forcing Due To a North Atlantic Pattern Effect
Authors: Mitevski Ivan , Dong Yue , Polvani Lorenzo M. , Rugenstein Maria , Orbe Clara
Published: July 2023

Effective climate sensitivity (EffCS), commonly estimated from model simulations with abrupt 4×CO2 for 150 years, has been shown to depend on the CO2 forcing level. To understand this dependency systematically, we performed a series of simulations with a range of abrupt CO2 forcing in two climate models. Our results indicate that normalized EffCS values in these simulations are a non‐monotonic function of the CO2 forcing, decreasing between 3× and 4×CO2 in CESM1‐LE (2× and 3×CO2 in GISS‐E2.1‐G) and increasing at higher CO2 levels. The minimum EffCS value, caused by anomalously negative radiative feedbacks, arises mainly from sea‐surface temperature (SST) relative cooling in the tropical and subtropical North Atlantic. This cooling is associated with the formation of the North Atlantic Warming Hole and Atlantic Meridional Overturning Circulation collapse under CO2 forcing. Our findings imply that understanding changes in North Atlantic SST patterns is important for constraining near‐future and equilibrium global warming.
Ensemble Spread Behavior in Coupled Climate Models: Insights From the Energy Exascale Earth System Model Version 1 Large Ensemble
Authors: Stevenson Samantha , Huang Xingying , Zhao Yingying , Di Lorenzo Emanuele , Newman Matthew , van Roekel Luke , Xu Tongtong , Capotondi Antonietta
Published: July 2023

Assessing uncertainty in future climate projections requires understanding both internal climate variability and external forcing. For this reason, single‐model initial condition large ensembles (SMILEs) run with Earth System Models (ESMs) have recently become popular. Here we present a new 20‐member SMILE with the Energy Exascale Earth System Model version 1 (E3SMv1‐LE), which uses a “macro” initialization strategy choosing coupled atmosphere/ocean states based on inter‐basin contrasts in ocean heat content (OHC). The E3SMv1‐LE simulates tropical climate variability well, albeit with a muted warming trend over the twentieth century due to overly strong aerosol forcing. The E3SMv1‐LE's initial climate spread is comparable to other (larger) SMILEs, suggesting that maximizing inter‐basin ocean heat contrasts may be an efficient method of generating ensemble spread. We also compare different ensemble spread across multiple SMILEs, using surface air temperature and OHC. The Community Earth system Model version 1, the only ensemble which utilizes a “micro” initialization approach perturbing only atmospheric initial conditions, yields lower spread in the first ∼30 years. The E3SMv1‐LE exhibits a relatively large spread, with some evidence for anthropogenic forcing influencing spread in the late twentieth century. However, systematic effects of differing “macro” initialization strategies are difficult to detect, possibly resulting from differing model physics or responses to external forcing. Notably, the method of standardizing results affects ensemble spread: control simulations for most models have either large background trends or multi‐centennial variability in OHC. This spurious disequlibrium behavior is a substantial roadblock to understanding both internal climate variability and its response to forcing.
Oblique Collision of the Bahamas Platform at the Northern Boundary of the Caribbean Plate Recorded by the Late Cenozoic Coastal Terraces of SE Cuba
Authors: Authemayou Christine , Nuñez Arelis , Pedoja Kevin , Peñalver Leandro , Chauveau Denovan , Dunán‐Avila Pedro , Martin‐Izquierdo Denyse , de Gelder Gino , Husson Laurent , Castellanos Abella Enrique , Benítez Frómeta Pedro de Jesús , Pastier Anne‐Morwenn
Published: July 2023

The southeastern tip of Cuba Island is limited to the south by the N‐Caribbean boundary. By revisiting the impressive sequences of coastal terraces of this region, we decipher the Quaternary deformation pattern of this plate boundary. We present a detailed mapping of coastal terraces uplifted over a hundred kilometers of coastline, and U/Th dating. At Punta de Maisí, the deformation pattern shows (a) a faster uplift close to the transform boundary and (b) a northward propagation of folding produced by the convergence of the Bahamas platform toward the Caribbean plate. Along the southern coast of Punta de Maisí, the sequence displays 29 coastal terraces up to 520 m in elevation and a upper Pleistocene uplift rate of 0.23 ± 0.07 mm yr−1. We interpret this deformation as resulting from an offshore north‐dipping reverse fault near the coast. This uplift rate corresponds to 3% to 1.6% of the short‐term horizontal slip rate of Septentrional Oriente Fault Zone (10 ± 0.1 mm yr−1). Along the northern coast of Punta de Maisí, the sequence displays height coastal terraces up to 220 m in elevation and the uplift rates amount to 0.1 ± 0.05 mm yr−1 and likely result from the reverse faulting and folding associated with the offshore North Hispaniola Fault Zone. Uplift rates quickly decrease to the West, in agreement with the westward decrease in the activity of the North Hispaniola Fault Zone due to the docking of the Bahamas Platform against Cuba, while the platform more gently underthrusts Cuba to the East.
Immunoglobulin genes expressed in lymphoblastoid cell lines discern and predict lithium response in bipolar disorder patients
Authors: Mizrahi Liron , Choudhary Ashwani , Ofer Polina , Goldberg Gabriela , Milanesi Elena , Kelsoe John R. , Gurwitz David , Alda Martin , Gage Fred H. , Stern Shani
Published: July 2023

Bipolar disorder (BD) is a neuropsychiatric mood disorder manifested by recurrent episodes of mania and depression. More than half of BD patients are non-responsive to lithium, the first-line treatment drug, complicating BD clinical management. Given its unknown etiology, it is pertinent to understand the genetic signatures that lead to variability in lithium response. We discovered a set of differentially expressed genes (DEGs) from the lymphoblastoid cell lines (LCLs) of 10 controls and 19 BD patients belonging mainly to the immunoglobulin gene family that can be used as potential biomarkers to diagnose and treat BD. Importantly, we trained machine learning algorithms on our datasets that predicted the lithium response of BD subtypes with minimal errors, even when used on a different cohort of 24 BD patients acquired by a different laboratory. This proves the scalability of our methodology for predicting lithium response in BD and for a prompt and suitable decision on therapeutic interventions.
How electroconvulsive therapy works in the treatment of depression: is it the seizure, the electricity, or both?
Authors: Deng Zhi-De , Robins Pei L. , Regenold William , Rohde Paul , Dannhauer Moritz , Lisanby Sarah H.
Published: July 2023

We have known for nearly a century that triggering seizures can treat serious mental illness, but what we do not know is why. Electroconvulsive Therapy (ECT) works faster and better than conventional pharmacological interventions; however, those benefits come with a burden of side effects, most notably memory loss. Disentangling the mechanisms by which ECT exerts rapid therapeutic benefit from the mechanisms driving adverse effects could enable the development of the next generation of seizure therapies that lack the downside of ECT. The latest research suggests that this goal may be attainable because modifications of ECT technique have already yielded improvements in cognitive outcomes without sacrificing efficacy. These modifications involve changes in how the electricity is administered (both where in the brain, and how much), which in turn impacts the characteristics of the resulting seizure. What we do not completely understand is whether it is the changes in the applied electricity, or in the resulting seizure, or both, that are responsible for improved safety. Answering this question may be key to developing the next generation of seizure therapies that lack these adverse side effects, and ushering in novel interventions that are better, faster, and safer than ECT.
RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles
Authors: Longo Michael A. , Roy Sunetra , Chen Yue , Tomaszowski Karl-Heinz , Arvai Andrew S. , Pepper Jordan T. , Boisvert Rebecca A. , Kunnimalaiyaan Selvi , Keshvani Caezanne , Schild David , Bacolla Albino , Williams Gareth J. , Tainer John A. , Schlacher Katharina
Published: July 2023

RAD51C is an enigmatic predisposition gene for breast, ovarian, and prostate cancer. Currently, missing structural and related functional understanding limits patient mutation interpretation to homology-directed repair (HDR) function analysis. Here we report the RAD51C-XRCC3 (CX3) X-ray co-crystal structure with bound ATP analog and define separable RAD51C replication stability roles informed by its three-dimensional structure, assembly, and unappreciated polymerization motif. Mapping of cancer patient mutations as a functional guide confirms ATP-binding matching RAD51 recombinase, yet highlights distinct CX3 interfaces. Analyses of CRISPR/Cas9-edited human cells with RAD51C mutations combined with single-molecule, single-cell and biophysics measurements uncover discrete CX3 regions for DNA replication fork protection, restart and reversal, accomplished by separable functions in DNA binding and implied 5’ RAD51 filament capping. Collective findings establish CX3 as a cancer-relevant replication stress response complex, show how HDR-proficient variants could contribute to tumor development, and identify regions to aid functional testing and classification of cancer mutations.
Glial Draper signaling triggers cross-neuron plasticity in bystander neurons after neuronal cell death in Drosophila
Authors: Wang Yupu , Zhang Ruiling , Huang Sihao , Valverde Parisa Tajalli Tehrani , Lobb-Rabe Meike , Ashley James , Venkatasubramanian Lalanti , Carrillo Robert A.
Published: July 2023

Neuronal cell death and subsequent brain dysfunction are hallmarks of aging and neurodegeneration, but how the nearby healthy neurons (bystanders) respond to the death of their neighbors is not fully understood. In the Drosophila larval neuromuscular system, bystander motor neurons can structurally and functionally compensate for the loss of their neighbors by increasing their terminal bouton number and activity. We term this compensation as cross-neuron plasticity, and in this study, we demonstrate that the Drosophila engulfment receptor, Draper, and the associated kinase, Shark, are required for cross-neuron plasticity. Overexpression of the Draper-I isoform boosts cross-neuron plasticity, implying that the strength of plasticity correlates with Draper signaling. In addition, we find that functional cross-neuron plasticity can be induced at different developmental stages. Our work uncovers a role for Draper signaling in cross-neuron plasticity and provides insights into how healthy bystander neurons respond to the loss of their neighboring neurons.
Construction of C-B axial chirality via dynamic kinetic asymmetric cross-coupling mediated by tetracoordinate boron
Authors: Yang Kai , Mao Yanfei , Zhang Zhihan , Xu Jie , Wang Hao , He Yong , Yu Peiyuan , Song Qiuling
Published: July 2023

Catalytic dynamic kinetic asymmetric transformation (DyKAT) provides a powerful tool to access chiral stereoisomers from racemic substrates. Such transformation has been widely employed on the construction of central chirality, however, the application in axial chirality remains underexplored because its equilibrium of substrate enantiomers is limited to five-membered metalacyclic intermediate. Here we report a tetracoordinate boron-directed dynamic kinetic asymmetric cross-coupling of racemic, configurationally stable 3-bromo-2,1-azaborines with boronic acid derivatives. A series of challenging C-B axially chiral compounds were prepared with generally good to excellent enantioselectivities. Moreover, this transformation can also be extended to prepare atropisomers bearing adjacent C-B and C-C diaxes with excellent diastereo- and enantio-control. The key to the success relies on the rational design of a reversible tetracoordinate boron intermediate, which is supported by theoretical calculations that dramatically reduces the rotational barrier of the original C-B axis and achieves the goal of DyKAT.
Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin
Authors: Chen Manyun , Shu Yan , Li Qing , Kang Zhiqiang , Liu Tao , Zhou Honghao , Huang Weihua , Zhang Wei
Published: July 2023

Vitamin B12 (VB12) deficiency, which may lead to hematologic and neurologic symptoms, has been associated with metformin use, but the underlying mechanism is unclear. Here we report the B. ovatus as an effective VB12 catcher which was enriched in the type 2 diabetes patients suffered from VB12 deficiency after 3 to 6 months of metformin treatment. Colonization of B. ovatus increased the plasma levels of methylmalonic acid and homocysteine in high-fat diet (HFD)-fed mice treated with metformin, and compromised the efficacy of metformin against the HFD-induced metabolic disorders. Mechanistically, metformin increased the intracellular accumulation of VB12 in B. ovatus via btuB upregulation and promoted ATP production for energy-dependent translocation of VB12 transporters at the inner membrane, leading to an enhanced colonization of B. ovatus to compete for VB12 with hosts and subsequently an aggravated VB12 deficiency in the host. Our findings illustrate a previously unappreciated mechanism of metformin leads to host VB12 deficiency by acting directly on gut bacteria to increase their VB12 uptake and consumption, and suggest that inter-host-microbe competition for nutrients may broadly impact human health and drug safety.
Chemical generation of checkpoint inhibitory T cell engagers for the treatment of cancer
Authors: Szijj Peter A. , Gray Melissa A. , Ribi Mikaela K. , Bahou Calise , Nogueira João C. F. , Bertozzi Carolyn R. , Chudasama Vijay
Published: July 2023

Bispecific T cell engagers (BiTEs), a subset of bispecific antibodies (bsAbs), can promote a targeted cancer cell’s death by bringing it close to a cytotoxic T cell. Checkpoint inhibitory T cell engagers (CiTEs) comprise a BiTE core with an added immunomodulatory protein, which serves to reverse cancer-cell immune-dampening strategies, improving efficacy. So far, protein engineering has been the main approach to generate bsAbs and CiTEs, but improved chemical methods for their generation have recently been developed. Homogeneous fragment-based bsAbs constructed from fragment antigen-binding regions (Fabs) can be generated using click chemistry. Here we describe a chemical method to generate biotin-functionalized three-protein conjugates, which include two CiTE molecules, one containing an anti-PD-1 Fab and the other containing an immunomodulatory enzyme, Salmonella typhimurium sialidase. The CiTEs’ efficacy was shown to be superior to that of the simpler BiTE scaffold, with the sialidase-containing CiTE inducing substantially enhanced T cell-mediated cytotoxicity in vitro. The chemical method described here, more generally, enables the generation of multi-protein constructs with further biological applications.
Trastuzumab deruxtecan in metastatic breast cancer with variable HER2 expression: the phase 2 DAISY trial
Authors: Mosele Fernanda , Deluche Elise , Lusque Amelie , Le Bescond Loïc , Filleron Thomas , Pradat Yoann , Ducoulombier Agnes , Pistilli Barbara , Bachelot Thomas , Viret Frederic , Levy Christelle , Signolle Nicolas , Alfaro Alexia , Tran Diep T. N. , Garberis Ingrid Judith , Talbot Hugues , Christodoulidis Stergios , Vakalopoulou Maria , Droin Nathalie , Stourm Aurelie , Kobayashi Maki , Kakegawa Tomoya , Lacroix Ludovic , Saulnier Patrick , Job Bastien , Deloger Marc , Jimenez Marta , Mahier Celine , Baris Vianney , Laplante Pierre , Kannouche Patricia , Marty Virginie , Lacroix-Triki Magali , Diéras Veronique , André Fabrice
Published: July 2023

The mechanisms of action of and resistance to trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate for breast cancer treatment, remain unclear. The phase 2 DAISY trial evaluated the efficacy of T-DXd in patients with HER2-overexpressing (n = 72, cohort 1), HER2-low (n = 74, cohort 2) and HER2 non-expressing (n = 40, cohort 3) metastatic breast cancer. In the full analysis set population (n = 177), the confirmed objective response rate (primary endpoint) was 70.6% (95% confidence interval (CI) 58.3–81) in cohort 1, 37.5% (95% CI 26.4–49.7) in cohort 2 and 29.7% (95% CI 15.9–47) in cohort 3. The primary endpoint was met in cohorts 1 and 2. Secondary endpoints included safety. No new safety signals were observed. During treatment, HER2-expressing tumors (n = 4) presented strong T-DXd staining. Conversely, HER2 immunohistochemistry 0 samples (n = 3) presented no or very few T-DXd staining (Pearson correlation coefficient r = 0.75, P = 0.053). Among patients with HER2 immunohistochemistry 0 metastatic breast cancer, 5 of 14 (35.7%, 95% CI 12.8–64.9) with ERBB2 expression below the median presented a confirmed objective response as compared to 3 of 10 (30%, 95% CI 6.7–65.2) with ERBB2 expression above the median. Although HER2 expression is a determinant of T-DXd efficacy, our study suggests that additional mechanisms may also be involved. (ClinicalTrials.gov identifier NCT04132960.)
Unravelling geospatial distribution and genetic diversity of greenhouse whitefly, Trialeurodes vaporariorum (Westwood) from Himalayan Region
Authors: Paschapur Amit Umesh , Singh Ashish Kumar , Buski Ramesh , Guru P. N. , Jeevan B. , Mishra K. K. , Kant Lakshmi
Published: July 2023

The Greenhouse whitefly (GWF), Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae), is a destructive pest that affects protected cultivation worldwide. The Indian Himalayan region is particularly vulnerable to GWF introduction, invasion, and spread due to the expansion of protected cultivation and climate change. In this study, we collected 32 naturally occurring GWF populations, mainly from the Uttarakhand state in the Indian Himalayan region, to investigate the distribution pattern and genetic diversity of T. vaporariorum. Our sampling was representative of the region's vegetation diversity and geographical location, and we collected samples from multiple sites within each locality to account for local variations. The mtCOI gene was used to accurately detect and identify GWF and to sequence haplotypes prevalent in the Uttarakhand state. The maximum likelihood method used for phylogenetic studies revealed that all 32 whitefly samples in this study belonged to T. vaporariorum and were prevalent in all the collected localities. Our population genetic study using mtCOI showed variation within T. vaporariorum populations, with 20 distinct haplotypes present. Notably, haplotype 2 (H2) was the most dominant haplotype among the sampled populations. These results provide fundamental knowledge for understanding the geographical distribution and ecology of T. vaporariorum in the Uttarakhand state of the Indian Himalayan region. The discovery of geospatial and genetic diversity of GWF in the Himalayan region underscores the importance of pest alertness, research prioritization, and the development of sustainable management strategies to protect crops.
Design and optimization of broadband metamaterial absorber based on manganese for visible applications
Authors: Sayed Shimaa I. , Mahmoud K. R. , Mubarak Roaa I.
Published: July 2023

Metamaterial absorbers have been extensively researched due to their potential applications in photonics. This paper presents a highly efficient Broadband Metamaterial Absorber (BMA) based on a Manganese–Silica–Manganese three layer structure with a shaped pattern at the top layer. For maximum absorption efficiency, the geometrical parameters of the proposed absorber have been optimized based on Particle Swarm Optimization (PSO). The optimal structure with a thickness of 190 nm, can achieve more than 94% absorption spanning visible band (400–800) nm with 98.72% average absorption, and more than 90% absorption over the range from 365 to 888 nm. In the range from 447 to 717 nm, the design presented above 99% absorptivity, providing an ultra-wide bandwidth of 270 nm. The physical mechanism of absorption is illustrated through the exploration of the electric and magnetic field distributions. Additionally, the proposed structure maintains 85% absorption stability for wide incident angles up to 70° for both the TE and TM polarizations under oblique incidence. Further, the optimized absorber structure with excellent absorption capabilities makes it suitable for various applications, including optical sensors, thermal emitters, and color imaging applications.
Introduction to the Monte Carlo dose engine COMPASS for BNCT
Authors: Zhong Wan-Bing , Chen Jiang , Teng Yi-Chiao , Liu Yuan-Hao
Published: July 2023

The Monte Carlo method is the most commonly used dose calculation method in the field of boron neutron capture therapy (BNCT). General-purpose Monte Carlo (MC) code (e.g., MCNP) has been used in most treatment planning systems (TPS) to calculate dose distribution, which takes overmuch time in radiotherapy planning. Based on this, we developed COMPASS (COMpact PArticle Simulation System), an MC engine specifically for BNCT dose calculation. Several optimization algorithms are used in COMPASS to make it faster than general-purpose MC code. The parallel computation of COMPASS is performed by the message passing interface (MPI) library and OpenMP commands, which allows the user to increase computational speed by increasing the computer configurations. The physical dose of each voxel is calculated for developing a treatment plan. Comparison results show that the computed dose distribution of COMPASS is in good agreement with MCNP, and the computational efficiency is better than MCNP. These results validate that COMPASS has better performance than MCNP in BNCT dose calculation.
Thermosensitive hydrogel coupled with sodium ascorbyl phosphate promotes human umbilical cord-derived mesenchymal stem cell-mediated skin wound healing in mice
Authors: Liu Liji , Yao Sheng , Mao Xianhua , Fang Zheng , Yang Cheng , Zhang Yan
Published: July 2023

Poor survival and restricted function of transplanted stem cells are regarded as limiting their efficacy in wound recovery greatly. Consequently, it is necessary to identify innovative therapeutic strategies to solve these issues. Firstly, the biological effect of PF-127 hydrogel alone and in combination with SAP on the survival, and migration of cultured HUCMSCs was assessed by cell viability, apoptosis, and scratch wound assays. S. aureus and E. coli were used to evaluate the antibacterial activity of PF-127 plus SAP combination. Further, the ability of HUCMSCs-conditioned medium (HUCMSCs-CM) to promote the angiogenesis and migration of human umbilical vein endothelial cells (HUVECs) in vitro was evaluated using tube formation and transwell migration assays. Finally, the HUCMSCs embedded in PF-127 plus SAP scaffold were administered onto mice’s excisional cutaneous wound bed. Histological and immunohistochemical analyses were employed to investigate the wound healing capacity as well as cellular responses of PF-127/HUCMSCs/SAP hydrogel. PF-127 showed cytotoxicity on HUCMSCs, whereas the addition of SAP significantly promoted cell viability and alleviated apoptosis of HUCMSCs encapsulated in PF-127 hydrogel in vitro. SAP supplementation substantially abrogated the inhibiting effect of PF-127 on the migration of HUCMSCs in vitro. The combination of PF-127 and SAP exerted an obvious bacteriostatic function on S. aureus and E. coli. Moreover, the co-treatment with SAP could remarkably enhance the stimulative effect of HUCMSCs-CM on the angiogenesis and migration of HUVECs in vitro. PF-127 combined SAP-embedded HUCMSCs transplantation resulted in a potently accelerated wound healing process, promoted the number of proliferating cells and newly formed blood vessels, as well as enhanced expression of vascular endothelial growth factor. PF-127 coupled with SAP contributes to HUCMSCs-mediated traumatic wound closure in mice by promoting cell survival, antibacterial action, and angiogenesis. Our results offered a theoretical foundation for the clinical treatment of traumatic skin defects.
Loss of Nudt15 thiopurine detoxification increases direct DNA damage in hematopoietic stem cells
Authors: Yamashita Noriaki , Kawahara Masahiro , Imai Takayuki , Tatsumi Goichi , Asai-Nishishita Ai , Andoh Akira
Published: July 2023

Thiopurines, such as 6-mercaptopurine (6-MP), are widely used as cytotoxic agents and immunosuppressants for leukemia and autoimmune or inflammatory diseases. A nonsynonymous single nucleotide polymorphism (p.Arg139Cys; R139C) of the nucleoside diphosphate-linked moiety X-type motif 15 (NUDT15) gene causes the loss of thiopurine detoxification, inducing myelosuppression. To understand such hematotoxicity, we investigate the effects of NUDT15 R139C on hematopoietic stem cells (HSCs) upon thiopurine administration. Using previously established Nudt15R138C knock-in mice, which mimic myelosuppression in NUDT15R139C homozygous or heterozygous patients following thiopurine administration, we investigated the numerical changes of HSCs and hematopoietic progenitor cells following 6-MP administration using in vivo flowcytometry and ex vivo HSC expansion. Genes differentially expressed between Nudt15+/+ HSCs and Nudt15R138C/R138C HSCs were identified using RNA-sequencing before the emergence of 6-MP-induced HSC-damage. Gene Ontology (GO) and Transcriptional Regulatory Relationships Unraveled by Sentence-based Text Mining (TRRUST) analyses were performed to elucidate the molecular effects of 6-MP on HSCs. In Nudt15R138C/R138C mice, 6-MP induced exhaustion of HSCs faster than that of multipotent progenitors and as fast as that of myeloid-committed progenitors. Ex vivo-expanded Nudt15R138C/R138C HSCs were dose- and time-dependently damaged by 6-MP. GO analysis identified the DNA damage response and cell cycle process as the most strongly influenced processes in Nudt15R138C/R138C HSCs. TRRUST analysis revealed that the Trp53-regulated transcriptional regulatory network is influenced prior to HSC exhaustion in Nudt15R138C/R138C HSCs. The loss of NUDT15 thiopurine detoxification enhances thiopurine-mediated DNA damage via the Trp53 networks in HSCs. Therefore, caution is required in long-term thiopurine use in patients with NUDT15 R139C in view of its adverse effects on HSCs in the form of DNA damage.
Effect of plant produced Anti-hIL-6 receptor antibody blockade on pSTAT3 expression in human peripheral blood mononuclear cells
Authors: Kaewbandit Namthip , Malla Ashwini , Boonyayothin Wanuttha , Rattanapisit Kaewta , Phetphoung Thareeya , Pisuttinusart Nuttapat , Strasser Richard , Saetung Rattana , Tawinwung Supannikar , Phoolcharoen Waranyoo
Published: July 2023

As a response to invasion by pathogens, the secretion of interleukin 6 (IL-6) which is a cytokine, activates IL-6/JAKs/STAT3 intracellular signaling via., phosphorylation. Over expression of pSTAT3 induces IL-6 positive feedback loop causing cytokine release syndrome or cytokine storm. Plants have gained momentum as an alternative expression system. Hence, this study aims to produce mAb targeting human IL-6 receptor (hIL-6R) in Nicotiana benthamiana for down regulating its cellular signaling thus, decreasing the expression of pSTAT3. The variable regions of heavy and light chains of anti-hIL-6R mAb were constructed in pBYK2e geminiviral plant expression vector and transiently co-expressed in N. benthamiana. The results demonstrate the proper protein assembly of anti-hIL-6R mAb with highest expression level of 2.24 mg/g FW at 5 dpi, with a yield of 21.4 µg/g FW after purification. The purity and N-glycosylation of plant produced antibody was analyzed, including its specificity to human IL-6 receptor by ELISA. Additionally, we investigated the effect to pSTAT3 expression in human PBMC’s by flow cytometry wherein, the results confirmed lower expression of pSTAT3 with increasing concentrations of plant produced anti-hIL-6R mAb. Although, further in vivo studies are key to unveil the absolute functionality of anti-hIL-6R, we hereby show the potential of the plant platform and its suitability for the production of this therapeutic antibody.
An extendable optical fibre probe survey meter for naturally occurring radioactive material (NORM) and other weak emitters
Authors: Zubair H. T. , Bradley D. A. , Khairina M. D. , Oresegun Adebiyi , Basaif A. , Othman J. , Rifiat R. , Hamidi F. , Rahman L. , Ezzadeen A. , Ibrahim S. A. , Mansor S. , Alkhorayef M. , Abdul-Rashid H. A.
Published: July 2023

We have developed a radioluminescence-based survey meter for use in industries in which there is involvement in naturally occurring radioactive material (NORM), also in support of those needing to detect other weak emitters of radiation. The functionality of the system confronts particular shortcomings of the handheld survey meters that are currently being made use of. The device couples a LYSO:Ce scintillator with a photodetector via a polymer optical fibre waveguide, allowing for "intrinsically safe" inspection within pipework, separators, valves and other such component pieces. The small-diameter optical fibre probe is electrically passive, immune to electromagnetic interference, and chemically inert. The readout circuit is entirely incorporated within a handheld casing housing a silicon photomultiplier (SiPM) detection circuit and a microprocessor circuit connected to an LCD display. A 15 m long flexible PMMA optical fibre waveguide is butt coupled to an ABS plastic probe that retains the LYSO:Ce scintillator. Initial tests have included the use of lab-based mixed gamma-ray sources, measurements being made in concert with a reference conventional GM survey-meter. Characterization, via NORM sources at a decontamination facility, has shown useful sensitivity, covering the dose-rate range 0.10- to 28 µSv h−1 (R-squared 0.966), extending to 80 µSv/h as demonstrated in use of a Cs-137 source. The system is shown to provide an effective tool for detection of radioactivity within hard to access locations, in particular for sources emitting at low radiation levels, down to values that approach background.
Effects of functionalization and silane modification of hexagonal boron nitride on thermal/mechanical/morphological properties of silicon rubber nanocomposite
Authors: Farahani Atefe , Jamshidi Masoud , Foroutan Masumeh
Published: July 2023

Hexagonal boron nitride (h-BN) nanoparticles could induce interesting properties to silicone rubber (SR) but, the weak filler-matrix interfacial interaction causes agglomeration of the nanoparticles and declines the performance of the nanocomposite. In this work, h-BN nanoparticles were surface modified using vinyltrimethoxysilane (VTMS) at different concentrations. Before silane modification, h-BN nanoparticles were hydroxylated using 5 molar sodium hydroxide. The nanoparticles were characterized to assess success of silane grafting. The pure and modified h-BN nanoparticles were applied at 1, 3 and 5 wt% to HTV silicon rubber (SR). The curing, thermal, mechanical and morphological properties and hydrophobicity of the nanocomposites were evaluated. The morphology of the SR nanocomposites was characterized using AFM and FE-SEM analysis. It was found that silane grafting on the h-BN nanoparticles improves crosslink density but declines curing rate index (CRI) of the SR nanocomposite (at 5 wt% loading content) by 0.7 (dN m) and 3.5%, respectively. It also increased water contact angle of the nanocomposites from 97.5° to 107°. The improved nanoparticle-rubber interfacial interactions caused better dispersion of h-BN nanoparticles in SR matrix (at 5 wt%) that enhanced the elongation at break, modulus at 300% and Tg of the SR nanocomposites.
Adsorption kinetics of methylene blue from wastewater using pH-sensitive starch-based hydrogels
Authors: Mohammadzadeh Fatemeh , Golshan Marzieh , Haddadi-Asl Vahid , Salami-Kalajahi Mehdi
Published: July 2023

In this work, starch/poly(acylic acid) hydrogels were synthesized through a free radical polymerization technique. The molar ratios of acrylic acid to N,N′-methylenebisacrylamide were 95:5, 94:6, and 93:7. The samples exhibited an amorphous porous structure, indicating that the size of the pores was contingent upon the amount of cross-linking agent. The quantity of acrylic acid in structure rose with a little increase in the amount of the cross-linking agent, which improved the hydrogels’ heat stability. The swelling characteristics of the hydrogels were influenced by both the pH level and the amount of cross-linking agent. The hydrogel with a ratio of 94:6 exhibited the highest degree of swelling (201.90%) at a pH of 7.4. The dominance of the Fickian effect in regulating water absorption in the synthesized hydrogels was demonstrated, and the kinetics of swelling exhibited agreement with Schott's pseudo-second order model. The absorption of methylene blue by the hydrogels that were developed was found to be influenced by various factors, including the concentration of the dye, the quantity of the cross-linking agent, the pH level, and the duration of exposure. The hydrogel 95:5 exhibited the highest adsorption effectiveness (66.7%) for the dye solution with a concentration of 20 mg/L at pH 10.0. The examination of the kinetics and isotherms of adsorption has provided evidence that the process of physisorption takes place on heterogeneous adsorbent surfaces and can be explained by an exothermic nature.
Quantifying the cooling effect of tropical cyclone clouds on the climate system
Authors: Hu Liang , Ritchie Elizabeth A. , Scott Tyo J.
Published: July 2023

The net effect on the upwelling radiation caused by tropical cyclone clouds is calculated over a 20-year global data set, and the corresponding contribution to the earth energy balance is analyzed. Tropical cyclone clouds are shown on average to increase the upwelling radiation at the top of the atmosphere compared with the background non-tropical-cyclone-cloud climatology. This increase in upwelling radiation provides an overall cooling effect on the climate system because the increased reflected shortwave radiation (cooling) outweighs the decreased emitted longwave radiation (warming). While the effect neglects the (likely considerable) contribution due to tropical cyclone drying, the amount of cooling by clouds alone represents a considerable fraction of the excess warming energy in the climate system. Thus, any future change in tropical cyclone activity has the potential to impact the overall energy balance if it substantially alters this total. The seasonal and geographic distribution of warming and cooling effects, and the diurnal dynamics that impact whether any particular cyclone is net cooling or net warming are discussed in this study.
Group size planning for breedings of gene-modified mice and other organisms following Mendelian inheritance
Authors: Milchevskaya Vladislava , Bugnon Philippe , ten Buren Emiel B. J. , Vanhecke Dominique , Brand Frank , Tresch Achim , Buch Thorsten
Published: July 2023

Colony management of gene-modified animals is time-consuming, costly and affected by random events related to Mendelian genetics, fertility and litter size. Careful planning is mandatory to ensure successful outcomes using the least number of animals, hence adhering to the 3R principles of animal welfare. Here we have developed an R package, accessible also through an interactive public website, that optimizes breeding design by providing information about the optimal number of breedings needed to obtain defined breeding outcomes, taking into account specific species, strain, or line properties and success probability. Our software also enables breeding planning for balanced male-to-female ratio or single-sex experiments. We show that, for single-sex designs, the necessary number of breedings is at least doubled compared to the use of all born animals. While the presented tool provides preset parameters for the laboratory mouse, it can be readily used for any other species.
Classification of formalin-fixed bladder cancer cells with laser tweezer Raman spectroscopy
Authors: Tang Nga Tsing , Robinson Richard , Snook Richard D. , Brown Mick , Clarke Noel , Gardner Peter
Published: July 2023

Raman optical tweezers identifies the invasive bladder cancer cell line, strengthening the argument for a spectroscopic based urine cytology test.
A novel electrochemical aptasensor based on polyaniline and gold nanoparticles for ultrasensitive and selective detection of ascorbic acid
Authors: Jiang Cuiwen , Xie Liping , Yan Feiyan , Liang Zhongdan , Liang Jing , Huang Kejing , Li Huiling , Wang Yanli , Luo Lihong , Li Tao , Ning Dejiao , Tang Li , Ya Yu
Published: July 2023

An ultrasensitive and selective electrochemical aptamer sensor based on a composite of polyaniline and gold nanoparticles was developed for detecting ascorbic acid.
Detection of SARS-CoV-2 in saliva by a low-cost LSPR-based sensor
Authors: Bido Ariadne Tuckmantel , Ember Katherine J. I. , Trudel Dominique , Durand Madeleine , Leblond Frederic , Brolo Alexandre G.
Published: July 2023

A biosensor based on AgNPs immobilized in plastic is used to detect infectious agents from saliva samples.
Fluorene-based tau fibrillation sensor and inhibitor with fluorogenic and photo-crosslinking properties
Authors: Xia Qiuxuan , Wang Zhiming , Wan Wang , Feng Huan , Sun Rui , Jing Biao , Ge Yusong , Liu Yu
Published: July 2023

Tau protein aggregation into neurofibrillary tangles often causes tauopathies.
Lewis acid-catalyzed formal 1,3-aminomethyl migration
Authors: Shibata Suzuka , Amano Koutarou , Kojima Tatsuhiro , Mori Keiji
Published: July 2023

Here we report a Lewis acid-catalyzed 1,3-aminomethyl migration rection.
Phase coexistence in [C22/C1MIm]+[NO3]− ionic-liquid mixtures and first-order phase transitions from homogeneous liquid to smectic B by varying the cation ratio
Authors: Yao Jie , Saielli Giacomo , Meng Fanlong , Wang Yanting
Published: July 2023

Two first-order phase transitions in the [C22/C1MIm]+[NO3]− ionic-liquid mixture separate three phases, which coexist during the second transition.
Atomic layer deposition of CoF2, NiF2 and HoF3 thin films
Authors: Atosuo Elisa , Mäntymäki Miia , Pesonen Leevi , Mizohata Kenichiro , Hatanpää Timo , Leskelä Markku , Ritala Mikko
Published: July 2023

In this work CoF2, NiF2 and HoF3 were deposited by atomic layer deposition (ALD) for the first time.
Ligand-dependent structural diversity and optimizable CO2chemical fixation activities of Cu-doped polyoxo-titanium clusters
Authors: Lin Xin , Yu Ying-Hua , Chen Guang-Hui , Li Qiao-Hong , Zhang Lei , Zhang Jian
Published: July 2023

A family of Cu-doped polyoxo-titanium clusters coordinated with different acetate derivative ligands were obtained, showing nuclearity and surface exposed Cu site-dependent CO2chemical fixation performance.
Understanding the impact of ammonium ion substitutions on heterogeneous ice nucleation
Authors: Blow Katarina E. , Whale Thomas F. , Quigley David , Sosso Gabriele C.
Published: July 2023

We investigate the changes in enthalpy and configurational entropy under the insertion of an ammonium ion into ice. For a reasonable surface charge the most energetically stable configuration of ammonium in ice increases the configurational entropy.
Selective deconstruction of mixed plastics by a tailored organocatalyst
Authors: Arifuzzaman Md , Sumpter Bobby G. , Demchuk Zoriana , Do Changwoo , Arnould Mark A. , Rahman Md Anisur , Cao Peng-Fei , Popovs Ilja , Davis Robert J. , Dai Sheng , Saito Tomonori
Published: July 2023

The developed highly efficient and versatile organocatalyst can deconstruct multiple condensation polymers selectively and sequentially into corresponding monomers, while keeping other polymers such as polyolefins or cotton intact.