bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of Surface Currents in the Adriatic Sea from Sequential Infrared Satellite Images
Authors: Notarstefano Giulio , Poulain Pierre-Marie , Mauri Elena
Published: March 2008

The maximum cross-correlation (MCC) technique is utilized to estimate the Adriatic Sea surface currents in regions characterized by strong horizontal temperature gradients using sequential pairs of sea surface temperature images from the Advanced Very High Resolution Radiometer data collected between September 2002 and December 2003. A variety of filtering techniques are used to eliminate erroneous MCC-derived currents resulting in velocity and direction estimates that are spatially coherent in most of the thermal features observed. The results are compared quantitatively to the currents measured by surface drifters and high-frequency coastal radars, operating simultaneously in the vicinity of the thermal structures considered. These comparisons show that surface MCC-derived velocities agree with the typical circulation pattern generally observed in the Adriatic basin. The MCC velocity estimates agree well with collocated and cotemporal drifter and radar measurements averaged on the time interval separating the pairs of images. Since the MCC method provides only estimates of surface currents when thermal features exist and are not covered by clouds, it is proposed that this technique be used preferably with other measurements of surface circulation (high-frequency coastal radars, drifters, etc.) to construct more accurate, more frequent, and more extended circulation maps for scientific and operational purposes in marginal seas such as the Adriatic.
Sensitivity of Surface Analyses over the Western United States to RAWS Observations
Authors: Myrick David T. , Horel John D.
Published: March 2008

Federal, state, and other wildland resource management agencies contribute to the collection of weather observations from over 1000 Remote Automated Weather Stations (RAWS) in the western United States. The impact of RAWS observations on surface objective analyses during the 2003/04 winter season was assessed using the Advanced Regional Prediction System (ARPS) Data Assimilation System (ADAS). A set of control analyses was created each day at 0000 and 1200 UTC using the Rapid Update Cycle (RUC) analyses as the background fields and assimilating approximately 3000 surface observations from MesoWest. Another set of analyses was generated by withholding all of the RAWS observations available at each time while 10 additional sets of analyses were created by randomly withholding comparable numbers of observations obtained from all sources.

Random withholding of observations from the analyses provides a baseline estimate of the analysis quality. Relative to this baseline, removing the RAWS observations degrades temperature (wind speed) analyses by an additional 0.5°C (0.9 m s−1) when evaluated in terms of rmse over the entire season. RAWS temperature observations adjust the RUC background the most during the early morning hours and during winter season cold pool events in the western United States while wind speed observations have a greater impact during active weather periods. The average analysis area influenced by at least 1.0°C (2.5°C) by withholding each RAWS observation is on the order of 600 km2 (100 km2). The spatial influence of randomly withheld observations is much less.
The Thermal Wake of Kauai Island: Satellite Observations and Numerical Simulations*
Authors: Yang Yang , Xie Shang-Ping , Hafner Jan
Published: March 2008

Island thermal effects on the trail cloud band over the central North Pacific are investigated for the lee of Hawaii using satellite observations and a regional atmospheric model. The trail cloud band develops around noon and peaks in cloudiness in the early afternoon. The analysis of numerical simulations of the Kauai wake suggests that a dynamically induced convergence zone forms in the lee of Kauai and Oahu (maximum elevation at 1.5 and 1.2 km, respectively) under the trade wind flow. The island thermal effect significantly modulates the island wake and creates a diurnal cycle of development and decay in the lee cloud band. As solar radiation heats up the island from morning to afternoon, warm air moves downstream (warm advection) from the island in the wake zone, increasing the air temperature, decreasing the air pressure, and enhancing low-level wind convergence in favor of the formation of the trail clouds. Conversely the cold advection during night suppresses cloud formation in the wake. The warm advection and the convergence in the wake increase with the upstream trade wind strength, consistent with satellite observations that the cloudiness increases in the wake under strong wind conditions in the afternoon.

The similarity in the trail cloud and its diurnal cycle between Kauai and Oahu suggests that the thermal wake effect is quite common. The conditions for such a thermal wake are discussed.
Verification of Quantitative Precipitation Forecasts via Stochastic Downscaling
Authors: Brussolo Elisa , von Hardenberg Jost , Ferraris Luca , Rebora Nicola , Provenzale Antonello
Published: March 2008

The use of dense networks of rain gauges to verify the skill of quantitative numerical precipitation forecasts requires bridging the scale gap between the finite resolution of the forecast fields and the point measurements provided by each gauge. This is usually achieved either by interpolating the numerical forecasts to the rain gauge positions, or by upscaling the rain gauge measurements by averaging techniques. Both approaches are affected by uncertainties and sampling errors due to the limited density of most rain gauge networks and to the high spatiotemporal variability of precipitation. For this reason, an estimate of the sampling errors is crucial for obtaining a meaningful comparison. This work presents the application of a stochastic rainfall downscaling technique that allows a quantitative comparison between numerical forecasts and rain gauge measurements, in both downscaling and upscaling approaches, and allows a quantitative assessment of the significance of the results of the verification procedure.
QuikSCAT Impacts on Coastal Forecasts and Warnings: Operational Utility of Satellite Ocean Surface Vector Wind Data
Authors: Milliff Ralph F. , Stamus Peter A.
Published: March 2008

This study reports on the operational utility of ocean surface vector wind (SVW) data from Quick Scatterometer (QuikSCAT) observations in the National Oceanic and Atmospheric Administration (NOAA) National Weather Service (NWS) Weather Forecast Offices (WFOs) covering the coastal United States, including island states and territories. Thirty-three U.S. coastal WFOs were surveyed, and 16 WFO site visits were conducted, from late summer 2005 to the 2005/06 winter season, in order to quantify the impact of QuikSCAT SVW data on forecasts and warnings, with a particular focus on operations affecting marine users. Details of the survey design and site visit strategies are described. Survey results are quantified and site visit impressions are discussed. Key findings include (i) QuikSCAT data supplement primary datasets and numerical weather prediction fields, in the manual production of local public (weather) and marine forecasts and warnings; (ii) operational utility of satellite SVW data would be enhanced by SVW retrievals of finer temporal resolution, closer to the coasts; and (iii) rain flags in the SVW data have little impact on utility for WFO operations.
Intravenous Port Catheter Embolization: Mechanisms, Clinical Features, and Management
Authors: Surov Alexey , Buerke Michael , John Endres , Kösling Sabrina , Spielmann Rolf-Peter , Behrmann Curd
Published: March 2008

Venous catheters are important therapeutic devices for the administration of fluid and chemotherapeutic agents; however, their use may be associated with serious complications, such as catheter rupture and embolism. Most data on port catheter embolization consist of isolated case reports; only a few studies have examined a large number of patients with port catheter embolism. The purpose of this study was to identify the incidence of clinical symptoms in patients with catheter dislocation and to determine the role of catheter fragment localization in combination with the presenting symptoms. We conducted a retrospective analysis of patients admitted to Martin-Luther University Hospital Center from January 1994 to September 2005. In total, 41 patients with centrally dislocated catheter fragments were analyzed. Most catheter fragments were located in the pulmonary artery, superior vena cava, and right atrium. Of the patients in whom the catheter fragments were located in the right atrium, right ventricle, and the pulmonary artery, 7.3% presented cardiac symptoms. Catheter malfunction occurred in 39%. In 53.7%, catheter embolism was found incidentally. The embolized catheter fragments were retrieved by a goose-neck snare under fluoroscopy within 24 hours after the diagnosis without any complications. The mean length of these fragments was 11.6 cm. Catheter embolism may go undiagnosed for a prolonged period and be found incidentally. In these patients, predominantly local symptoms occur; however, severe systemic clinical signs may develop. The risk of serious complications in asymptomatic catheter embolism is unknown. Catheter fragments should be removed to prevent further complications.
Angiographic Restenosis in Ephesos Coronary Stents: Experience From a Large Medical Center in Ankara, Turkey
Authors: Altin Timucin , Berkalp Berkten , Ozdol Çagdas , Akyurek Omer , Sayin Tamer , Kervancioglu Celal , Oral Dervis , Erol Cetin
Published: March 2008

Coronary stent restenosis, which emerges in late periods after implantation, has not been completely abolished. Our aim was to investigate the restenosis rates of Ephesos coronary stents. In all, 96 patients (66 men) with 135 Ephesos coronary stents were included. Control angiograms were performed after 160 ± 60 days. Quantitative coronary analysis was performed during the procedure and control angiogram. The stents were divided into 2 groups according to the presence or absence of restenosis. Groups were compared with clinical and angiographic variables. Restenosis was observed in 31 (23%) of 135 stents. Preprocedure percent diameter stenosis was higher ( P = .02), whereas minimum lumen diameter ( P = .02), mean age ( P < .001), and hypertension incidence ( P = .043) was less, and there was a trend toward smaller stent size ( P = .054) in the restenosis group. By multivariate analysis, age <50 years ( P < .001) and stent size <3.0 mm ( P = .016) were independent predictors of restenosis. Ephesos coronary stents seems to have acceptable restenosis rates.
Dolichoectatic Cervical Arteries (Carotid and Vertebral Arteries) Heralded by Recurrent Cerebral Ischemia: Case Illustration
Authors: Mourgela Sofia , Anagnostopoulou S. , Sakellaropoulos Antonios , Spanos A.
Published: March 2008

A case of a 61-year-old man with recurrent episodes of cerebral transient ischemic attacks is reported. The patient had a history of cigarette smoking, hypertension, hypercholesterolemia, and diabetes mellitus. Before these episodes, the patient had no clinical symptoms and signs of cerebral pathology. Brain magnetic resonance imaging revealed microvascular lesions in the white matter of the cerebral hemispheres. Digital subtraction arteriogram revealed the aortic arch dolichoic shape and course of the great vessels originating from it, whereas there were no pathological findings from the intracranial vessels. This study describes a case of the existence of distal dolichoectasia of the vertebral and carotid arteries without intracranial dolichoectasia. It seems that such a type of dolichoectasia does not influence the performance status of a patient, but when a critical point is crossed, patients suffer from cerebrovascular disease.
NPM and beyond — structure, culture and demography
Authors: Christensen Tom , Lægreid Per
Published: March 2008

The main research questions in this article are to what degree New Public Management (NPM) and post-NPM reform elements have been perceived as relevant or significant in the Norwegian civil service; what have been the most significant reform elements; and how to explain the variation in the perceived significance of different administrative reform tools. How important is leadership relative to other structural factors and to demographic and cultural features? The empirical data used in the analysis are taken mainly from surveys of civil servants in the Norwegian ministries conducted in 1996 and 2006. A general finding is that cultural features make a significant difference, but having a leadership position and task structure also have explanatory power. Generally, we face a combination of robustness and amenability to administrative policy reforms.

Points for practitioners

This article shows that the perceived significance of different administrative reform tools is high within government ministries in Norway. Four families of reform measures are revealed: performance-management reforms, cultural-managerial reforms, structural reforms and market-related reforms. Reform measures connected to performance management systems generally have high scores, while market-related reforms score low. The administrative reforms show a robust pattern from 1996 to 2006, but new reform tools are added to existing measures and this produces increased complexity. The variations in significance of different reform tools can be traced back to administrative culture, leadership position and main tasks.
Uncertainty Orientation and Affective Experiences
Authors: Sorrentino Richard M. , Nezlek John B. , Yasunaga Satoru , Kouhara Sadafusa , Otsubo Yasunao , Shuper Paul
Published: March 2008

This study examined whether people with the same self-regulatory style of coping with uncertainty differed in their affective experiences as a function of what may be the dominant coping style of their culture. Two hundred twenty men and women from universities in Japan and Canada described the extent to which they experienced various emotions. Consistent with their expectations, the authors found that participants whose uncertainty orientation matched that of their country (i.e., uncertainty-oriented students in Canada, certainty-oriented students in Japan) experienced more active emotions than mismatched participants (i.e., certainty-oriented students in Canada, uncertainty-oriented students in Japan), who experienced more passive emotions. Moreover, those who matched their country's coping style also reported experiencing more positive and fewer negative emotions than mismatched students. These results suggest that the theory of uncertainty orientation has important implications for research on affective experiences within and across cultures.
Effectiveness of a Mexican Health Education Program in a Poverty-Stricken Rural Area of Guatemala
Authors: Leenen Iwin , Givaudan Martha , Pick Susan , Venguer Tere , Vera Judith , Poortinga Ype H.
Published: March 2008

In this article, the authors discuss the transfer to Guatemala of an integral health education program, originally developed for indigenous women in southern Mexico. The program was implemented with some 400 indigenous women in rural Guatemala living under dire poverty, and was carried out through a closely supervised cascade process in which specially trained local women conducted workshops to their fellow countrywomen. The program aimed at imparting knowledge as well as enabling changes in behavior with respect to everyday life issues, including nutrition, hygiene, sanitation, and sexual and reproductive health. Evaluation of the impact through questionnaires and direct observations showed significant increases in knowledge and actual behaviors.
Liposomal Nanomedicines: An Emerging Field
Authors: Fenske David B. , Chonn Arcadio , Cullis Pieter R.
Published: March 2008

Liposomal nanoparticles (LNs) encapsulating therapeutic agents, or liposomal nanomedicines (LNMs), represent one of the most advanced classes of drug delivery systems, with several currently on the market and many more in clinical trials. During the past 20 years, a variety of techniques have been developed for encapsulating both conventional drugs and the new genetic drugs (plasmid DNA–containing therapeutic genes, anti-sense oligonucleotides, and small, interfering RNA [siRNA]) within LNs encompassing a very specific set of properties: a diameter centered on 100 nm, a high drug-to-lipid ratio, excellent retention of the encapsulated drug, and a long (> 6 hours) circulation lifetime. Particles with these properties tend to accumulate at sites of disease, such as tumors, where the endothelial layer is “leaky” and allows extravasation of particles with small diameters. Thus, LNs protect the drug during circulation, prevent it from reaching healthy tissues, and permit its accumulation at sites of disease. We will discuss recent advances in this field involving conventional anticancer drugs as well as gene-delivery, immunostimulatory, and gene-silencing applications involving the new genetic drugs. LNMs have the potential to offer new treatments in such areas as cancer therapy, vaccine development, and cholesterol management.
Are Involved Parents Providing Public Goods or Private Goods?
Authors: Walsh Patrick
Published: March 2008

Parents who lobby their children's schools for better or increased educational resources may be providing a schoolwide public good. However, they may also be capturing a larger share of school resources, possibly leaving other families worse off. A regression of within-school variation in input quality on parent-initiated school contact identifies this ``private-good'' effect, using involvement in civic organizations to instrument for contact. A 10 percent increase in the probability of parental contact raises the probability of inclusion in a gifted program by 2 percent to 3 percent. At the average school, the highest ``non-gifted'' students significantly outscore the lowest ``gifted'' students, suggesting that this lobbying crowds out deserving students. Alongside these private-good effects, involved parents also provide public goods: again instrumenting with civic involvement, higher participation in a school's parent—teacher organization leads to a higher probability that principals frequently observe and evaluate all teachers at that school.
Editorial Judgments, Quality Scholarship, and the Academy of Management's Journals
Authors: Hitt Michael A.
Published: March 2008

Bedeian, Van Fleet, and Hyman's article offers a critical examination of the scholarly achievements of editorial review board members for several scholarly journals in related disciplines, including the Academy of Management's primary scholarly journals, the Academy of Management Review and the Academy of Management Journal (AMJ). The author finds their critique to be interesting and thorough in many ways. However, this commentary examines several issues related to their critique, including the methodology, criteria for and process of selecting editorial review board members, the editor's role in the evaluation of work submitted to journals, and the performance of scholarly journals. Consideration of these issues allows a broader and more complete view of scholarly publishing, leading to some different conclusions.
Damage Detection on Stay Cables using an Open Source-based Framework for Finite Element Model Updating
Authors: Mordini Andrea , Savov Konstantin , Wenzel Helmut
Published: March 2008

An open source software-based solution for Finite Element Model Updating is presented. The calculation technique has been carried out by developing VCUPDATE, a Scilab (MATLAB-like software) code performing the iterative updating algorithm interfaced with OpenSees executing the Finite Element analysis. The code has been verified by many different applications on stay cables. The dynamic test results from real structures are applied if available. Otherwise, the investigated structures are numerically generated by OpenSees.
Merging Sensor Data from Multiple Temperature Scenarios for Vibration Monitoring of Civil Structures
Authors: Balmès Étienne , Basseville Michèle , Bourquin Frédéric , Mevel Laurent , Nasser Houssein , Treyssède Fabien
Published: March 2008

The ambient temperature effect may result in limitations of vibration-based SHM approaches for civil engineering structures. This paper addresses the issue of discriminating changes in modal parameters due to damages and changes in modal parameters due to temperature effects. A nonparametric damage detection algorithm is proposed, which only assumes that several data sets are recorded on the safe structure at different and unknown temperatures, and smoothes out the temperature effect using an averaging operation.
Getting Past the Gatekeepers: Differences Between Access and Cooperation in Public School Research
Authors: Wanat Carolyn L.
Published: March 2008

This article describes differences in access and cooperation in four qualitative research studies in public schools. Gatekeepers grant formal access but withhold cooperation if they think studies threaten them or their schools. Project approval by official gatekeepers does not guarantee cooperation from informal gatekeepers and participants. The author describes gatekeepers' practices to grant or withhold access and cooperation and the influence of perceived benefits and threats to cooperation. The article concludes with lessons from these research experiences that may lead to more cooperative relationships between researchers and gatekeepers.
A Fantasy Made Real
Authors: Metz Anneke M.
Published: March 2008

The need for drama in the contemporary television science documentary, coupled with the advent of computer-generated imaging (CGI) technology, has led to the development of increasingly fiction-driven science documentary, specifically on cable outlets such as the Discovery suite channels and National Geographic. While subjunctive documentary shows such as Walking with Dinosaurs initially used CGI technology to illustrate scientists' best understanding of Earth prehistory, the use of CGI has quickly escalated to the animation of purely fantastical life forms. This trend has resulted in a baroque subjunctive documentary form that has evacuated any possibility of serious science documentary on cable channels devoted to science programming.
“An . . . Unmarried Mother Sat in a Wing-Backed Chair on TV Last Night . . .”
Authors: Holmes Su
Published: March 2008

In 1955, Daily Mirror reported, “An unmarried mother hid her face on TV last night. . . .” The headline referred to the BBC's Is This Your Problem? ( ITYP? 1955—57), in which members of the public sought the help of an “expert” panel. Based on archival research, this article situates ITYP? in relation to the historical circumstances of its circulation, exploring what it can contribute to knowledge of British television's aesthetic development and production of cultural identity and how these were negotiated by the BBC. In precariously negotiating public service values and popular appeal, ITYP? is interesting for prefiguring debates about television's “tabloidization,” and the program's reception speaks to the cultural debate that greeted the talk show in the 1990s. While there is always a danger of fostering an “undue proximity” between past and present, ITYP? offers a valuation contribution to the genealogy of these debates, particularly in elucidating television's (re)negotiation of public—private spheres.
Clocking Gender Differences
Authors: Billings Andrew C.
Published: March 2008

Analysis of all 348 prime-time hours of the 1996—2006 Olympic telecasts (three Summer, three Winter) pinpointed trends in coverage of men's and women's sports. Results indicate that while men athletes and events received the majority of clock time in all six Olympic telecasts, the Summer Olympic telecasts treated women far more equitably than the Winter Olympic telecast. The longitudinal study does not offer any reason to feel that coverage of women's athletics is improving over time, finding that the proportion of clock time devoted to men's and women's sports is relatively the same in 2006 compared to ten years earlier.
Treatment of Spontaneous Lumbar Artery Pseudoaneurysm by Computed Tomography—Guided Thrombin Injection
Authors: Sharma Devesh , Elmore James R. , Nadal Luis L. , Sheldon David G. , Franklin David P.
Published: March 2008

A case of spontaneous pseudoaneurysm of a lumbar artery that was treated with computed tomography— guided thrombin injection is reported in this study. To the authors' knowledge, pseudoaneurysm of lumbar artery without any predisposing factors has been reported only twice in the literature previously. The aims of this case report are to discuss the differential diagnosis and management of this difficult problem.
Mediating solidarity
Authors: Fenton Natalie
Published: March 2008

With the apparent increase in the number of alternative political media, political pluralists are again faced with the question: does the proliferation of subaltern counter-publics lead to a multiplication of forces? Fragmentation in political culture is fuelled by the rise of identity politics that focuses on consumption not production. Party allegiances and class alliances give way to more fluid and informal networks of action. Postmodern theorists celebrate fragmentation because it allows the recognition of diversity in political desires, acknowledges difference between individuals and debunks the myth of homogenous political units leading ultimately to liberation. But for political efficacy there must be more than the apparent freedom that comes with embracing difference and diversity. This article argues that if we accept the description of society as fragmented, in order to create a viable political community then solidarity is crucial. In a global economy, solidarity can be mediated through new communication technologies but the challenge is to articulate the politics online with actual movements and struggles on the ground.
Environmental and genetic modifiers of the progression to fibrosis and cirrhosis in hemochromatosis
Authors: Wood Marnie J. , Powell Lawrie W. , Ramm Grant A.
Published: March 2008

Hereditary hemochromatosis is a genetic disorder of iron metabolism leading to inappropriate iron absorption and iron loading in various organs especially the liver. Despite the genetic mutation being relatively common in those of Anglo Celtic descent, cirrhosis of the liver occurs in only a small proportion of affected individuals. The risk of hepatic fibrosis and cirrhosis relates to the degree of iron loading with threshold hepatic iron concentrations being identified from population studies. However, other environmental and possibly genetic factors appear to modify this risk. Excess alcohol consumption appears to be one of the most important cofactors with steatosis and coexistent viral infection also implicated. Genetic polymorphisms in genes associated with fibrogenesis, antioxidant activity, and inflammation have been investigated in several different forms of chronic liver disease. The variability in the expression of these genes that predispose patients with hemochromatosis to increased risk of severe liver disease is the subject of ongoing investigations. Clearly the progression of iron loading to cirrhosis marks a crucial stage in the natural history of a patient's disease and therefore therapy and prognosis. This review explores recent developments in knowledge of environmental and genetic modifiers of this process.
Evolution of a family of metazoan active-site-serine enzymes from penicillin-binding proteins: a novel facet of the bacterial legacy
Authors: Peitsaro Nina , Polianskyte Zydrune , Tuimala Jarno , Pörn-Ares Isabella , Liobikas Julius , Speer Oliver , Lindholm Dan , Thompson James , Eriksson Ove
Published: March 2008

Background

Bacterial penicillin-binding proteins and β-lactamases (PBP-βLs) constitute a large family of serine proteases that perform essential functions in the synthesis and maintenance of peptidoglycan. Intriguingly, genes encoding PBP-βL homologs occur in many metazoan genomes including humans. The emerging role of LACTB, a mammalian mitochondrial PBP-βL homolog, in metabolic signaling prompted us to investigate the evolutionary history of metazoan PBP-βL proteins.

Results

Metazoan PBP-βL homologs including LACTB share unique structural features with bacterial class B low molecular weight penicillin-binding proteins. The amino acid residues necessary for enzymatic activity in bacterial PBP-βL proteins, including the catalytic serine residue, are conserved in all metazoan homologs. Phylogenetic analysis indicated that metazoan PBP-βL homologs comprise four alloparalogus protein lineages that derive from α-proteobacteria.

Conclusion

While most components of the peptidoglycan synthesis machinery were dumped by early eukaryotes, a few PBP-βL proteins were conserved and are found in metazoans including humans. Metazoan PBP-βL homologs are active-site-serine enzymes that probably have distinct functions in the metabolic circuitry. We hypothesize that PBP-βL proteins in the early eukaryotic cell enabled the degradation of peptidoglycan from ingested bacteria, thereby maximizing the yield of nutrients and streamlining the cell for effective phagocytotic feeding.
Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages
Authors: Mian MA Rouf , Zhang Yan , Wang Zeng-Yu , Zhang Ji-Yi , Cheng Xiaofei , Chen Lei , Chekhovskiy Konstantin , Dai Xinbin , Mao Chunhong , Cheung Foo , Zhao Xuechun , He Ji , Scott Angela D , Town Christopher D , May Gregory D
Published: March 2008

Background

Tall fescue (Festuca arundinaceaSchreb) is a major cool season forage and turf grass species grown in the temperate regions of the world. In this paper we report the generation of a tall fescue expressed sequence tag (EST) database developed from nine cDNA libraries representing tissues from different plant organs, developmental stages, and abiotic stress factors. The results of inter-library and library-specificin silicoexpression analyses of these ESTs are also reported.

Results

A total of 41,516 ESTs were generated from nine cDNA libraries of tall fescue representing tissues from different plant organs, developmental stages, and abiotic stress conditions. TheFestucaGene Index (FaGI) has been established. To date, this represents the first publicly available tall fescue EST database.In silicogene expression studies using these ESTs were performed to understand stress responses in tall fescue. A large number of ESTs of known stress response gene were identified from stressed tissue libraries. These ESTs represent gene homologues of heat-shock and oxidative stress proteins, and various transcription factor protein families. Highly expressed ESTs representing genes of unknown functions were also identified in the stressed tissue libraries.

Conclusion

FaGI provides a useful resource for genomics studies of tall fescue and other closely related forage and turf grass species. Comparative genomic analyses between tall fescue and other grass species, including ryegrasses (Loliumsp.), meadow fescue (F. pratensis) and tetraploid fescue (F. arundinacea var glaucescens) will benefit from this database. These ESTs are an excellent resource for the development of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) PCR-based molecular markers.
Identification of Single Nucleotide Polymorphisms and analysis of Linkage Disequilibrium in sunflower elite inbred lines using the candidate gene approach
Authors: Fusari Corina M , Lia Verónica V , Hopp H Esteban , Heinz Ruth A , Paniego Norma B
Published: March 2008

Background

Association analysis is a powerful tool to identify gene loci that may contribute to phenotypic variation. This includes the estimation of nucleotide diversity, the assessment of linkage disequilibrium structure (LD) and the evaluation of selection processes. Trait mapping by allele association requires a high-density map, which could be obtained by the addition of Single Nucleotide Polymorphisms (SNPs) and short insertion and/or deletions (indels) to SSR and AFLP genetic maps. Nucleotide diversity analysis of randomly selected candidate regions is a promising approach for the success of association analysis and fine mapping in the sunflower genome. Moreover, knowledge of the distance over which LD persists, in agronomically meaningful sunflower accessions, is important to establish the density of markers and the experimental design for association analysis.

Results

A set of 28 candidate genes related to biotic and abiotic stresses were studied in 19 sunflower inbred lines. A total of 14,348 bp of sequence alignment was analyzed per individual. In average, 1 SNP was found per 69 nucleotides and 38 indels were identified in the complete data set. The mean nucleotide polymorphism was moderate (θ = 0.0056), as expected for inbred materials. The number of haplotypes per region ranged from 1 to 9 (mean = 3.54 ± 1.88). Model-based population structure analysis allowed detection of admixed individuals within the set of accessions examined. Two putative gene pools were identified (G1 and G2), with a large proportion of the inbred lines being assigned to one of them (G1). Consistent with the absence of population sub-structuring, LD for G1 decayed more rapidly (r2 = 0.48 at 643 bp; trend line, pooled data) than the LD trend line for the entire set of 19 individuals (r2 = 0.64 for the same distance).

Conclusion

Knowledge about the patterns of diversity and the genetic relationships between breeding materials could be an invaluable aid in crop improvement strategies. The relatively high frequency of SNPs within the elite inbred lines studied here, along with the predicted extent of LD over distances of 100 kbp (r2~0.1) suggest that high resolution association mapping in sunflower could be achieved with marker densities lower than those usually reported in the literature.
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography
Authors: Sewekow Eva , Kessler Lars Christian , Seidel-Morgenstern Andreas , Rothkötter Hermann-Josef
Published: March 2008

Background

Soybeans play a prominent role in allergologic research due to the high incidence of allergic reactions. For detailed studies on specific proteins it is necessary to have access to a large amount of pure substance.

Results

In this contribution, a method for purifying soybean (Glycine max) protein P34 (also called Gly m Bd 30 K or Gly m 1) using hydrophobic interaction chromatography is presented. After screening experiments using 1 mL HiTrap columns, Butyl Sepharose 4 FF was selected for further systematic investigations. With this stationary phase, suitable operation conditions for two-step gradient elution using ammonium sulphate were determined experimentally. The separation conditions obtained in a small column could be scaled up successfully to column volumes of 7.5 and 75 mL, allowing for high product purities of almost 100% with a yield of 27% for the chromatographic separation step. Conditions could be simplified further using a onestep gradient, which gave comparable purification in a shorter process time. The identity of the purified protein was verified using in-gel digestion and mass spectrometry as well as immunological techniques.

Conclusion

With the technique presented it is possible to produce, within a short timeframe, pure P34, suitable for further studies where an example antigen is needed.
Variability in prescription drug expenditures explained by adjusted clinical groups (ACG) case-mix: A cross-sectional study of patient electronic records in primary care
Authors: Aguado Alba , Guinó Elisabet , Mukherjee Bhramar , Sicras Antoni , Serrat Josep , Acedo Mateo , Ferro Juan Jose , Moreno Victor
Published: March 2008

Background

In view of rapidly increasing prescription costs, case-mix adjustment should be considered for effective control of costs. We have estimated the variability in pharmacy costs explained by ACG in centers using patient electronic records, profiled centers and physicians and analyzed the correlation between cost and quality of prescription.

Methods

We analyzed 65,630 patient records attending five primary care centers in Spain during 2005. Variables explored were age, gender, registered diagnosed episodes of care during 2005, total cost of prescriptions, physician and center. One ACG was assigned to each patient with ACG case-mix software version 7.1. In a two-part model, logistic regression was used to explain the incurrence of drug expenditure at the first stage and a linear mixed model that considered the multilevel structure of data modeled the cost, conditional upon incurring any expense. Risk and efficiency indexes in pharmacy cost adjusted for ACG were obtained for centers and physicians. Spearman rank correlation between physician expenditure, adjusted for ACG, and a prescription quality index was also obtained. Pediatric and adult data were analyzed separately.

Results

No prescription was recorded for 13% of adults and 39.6% of children. The proportion of variance of the incurrence of expenditure explained by ACGs was 0.29 in adults and 0.21 in children. For adults with prescriptions, the variance of cost explained by ACGs was 35.4%, by physician-center was 1.8% and age 10.5% (residual 52.3%). For children, ACGs explained 22.4% of cost and physician-center 10.9% (residual 66.7%). Center efficiency index for adults ranged 0.58 to 1.22 and for children 0.32 to 2.36.

Spearman correlation between expenditure and prescription quality index was -0.36 in family physicians (p = 0.019, N = 41) and -0.52 in pediatricians (p = 0.08, N = 12).

Conclusion

In our setting, ACG is the variable studied that explains more variability in pharmacy cost in adults compared to physician and center. In children there is greater variability among physicians and centers not related to case-mix. In our sites, ACG is useful to profile physicians and centers using electronic records in real practical conditions. Physicians with lower pharmaceutical expenditure have higher scores for a prescription quality index.
A quantitative method for determining spatial discriminative capacity
Authors: Zhang Zheng , Tannan Vinay , Holden Jameson K , Dennis Robert G , Tommerdahl Mark
Published: March 2008

Background

The traditional two-point discrimination (TPD) test, a widely used tactile spatial acuity measure, has been criticized as being imprecise because it is based on subjective criteria and involves a number of non-spatial cues. The results of a recent study showed that as two stimuli were delivered simultaneously, vibrotactile amplitude discrimination became worse when the two stimuli were positioned relatively close together and was significantly degraded when the probes were within a subject's two-point limen. The impairment of amplitude discrimination with decreasing inter-probe distance suggested that the metric of amplitude discrimination could possibly provide a means of objective and quantitative measurement of spatial discrimination capacity.

Methods

A two alternative forced-choice (2AFC) tracking procedure was used to assess a subject's ability to discriminate the amplitude difference between two stimuli positioned at near-adjacent skin sites. Two 25 Hz flutter stimuli, identical except for a constant difference in amplitude, were delivered simultaneously to the hand dorsum. The stimuli were initially spaced 30 mm apart, and the inter-stimulus distance was modified on a trial-by-trial basis based on the subject's performance of discriminating the stimulus with higher intensity. The experiment was repeated via sequential, rather than simultaneous, delivery of the same vibrotactile stimuli.

Results

Results obtained from this study showed that the performance of the amplitude discrimination task was significantly degraded when the stimuli were delivered simultaneously and were near a subject's two-point limen. In contrast, subjects were able to correctly discriminate between the amplitudes of the two stimuli when they were sequentially delivered at all inter-probe distances (including those within the two-point limen), and improved when an adapting stimulus was delivered prior to simultaneously delivered stimuli.

Conclusion

Subjects' capacity to discriminate the amplitude difference between two vibrotactile stimulations was degraded as the inter-stimulus distance approached the limit of their two-point spatial discriminative capacity. This degradation of spatial discriminative capacity lessened when an adapting stimulus was used. Performance of the task, as well as improvement on the task with adaptation, would most likely be impaired if the cortical information processing capacity of a subject or subject population were systemically altered, and thus, the methods described could be effective measures for use in clinical or clinical research applications.
Acute effects of caffeine and cigarette smoking on ventricular long-axis function in healthy subjects
Authors: Giacomin Elisa , Palmerini Elisabetta , Ballo Piercarlo , Zacà Valerio , Bova Giovanni , Mondillo Sergio
Published: March 2008

Background

Few data exist regarding the direct effects of caffeine and smoking on cardiac function. We sought to explore the acute effects of caffeine assumption, cigarette smoking, or both on left ventricular (LV) and right ventricular (RV) function in a population of young normal subjects.

Methods

Forty-five healthy subjects aged 25 ± 2 years underwent echocardiography. Fifteen of them were non-smokers and habitual coffee consumers (group 1), 15 were smokers and not habitual coffee consumers (group 2), and 15 were smokers and habitual coffee consumers (group 3). Peak systolic (Sa), early diastolic Ea, and late diastolic (Aa) velocity of mitral annulus were measured by pulsed Tissue Doppler, and left atrioventricular plane displacement was determined by M-mode. Tricuspid annular velocities and systolic excursion (TAPSE) were also determined. Measurements were performed at baseline and after oral assumption of caffeine 100 mg in group 1, one cigarette smoking in group 2, and both in group 3.

Results

No changes in ventricular function were observed in group 1 after caffeine administration. In group 2, cigarette smoking yielded an acute increase in mitral Aa (+12.1%, p = 0.0026), tricuspid Sa (+9.8%, p = 0.012) and TAPSE (+7.9%, p = 0.017), and a decrease in the mitral Ea/Aa ratio (-8.5%, p = 0.0084). Sequential caffeine assumption and cigarette smoking in group 3 was associated with an acute increase in mitral Aa (+13.0%, p = 0.015) and tricuspid Aa (+11.6%, p < 0.0001) and a reduction in mitral Ea/Aa ratio (-8.5%, p = 0.0084) tricuspid Ea (-6.6%, p = 0.048) and tricuspid Ea/Aa ratio (-9.6%, p = 0.0003). In a two-way ANOVA model controlling for hemodynamic confounding factors, changes in the overall population remained significant for mitral Aa and Ea/Aa ratio, and for tricuspid Aa and Ea/Aa ratio.

Conclusion

In young healthy subjects, one cigarette smoking is associated to an acute impairment in LV diastolic function and a hyperdynamic RV systolic response. Caffeine assumption alone does not exert any acute effect on ventricular long-axis function, but potentiates the negative effect of cigarette smoking by abolishing RV supernormal response and leading to a simultaneous impairment in both LV and RV diastolic function.
Antenatal counseling on breastfeeding – is it adequate? A descriptive study from Pondicherry, India
Authors: Dhandapany Gunasekaran , Bethou Adhisivam , Arunagirinathan Arulkumaran , Ananthakrishnan Shanthi
Published: March 2008

Background

Antenatal counseling on breastfeeding and postnatal lactation support are likely to improve rates of exclusive breastfeeding. This descriptive study was undertaken to assess whether antenatal visits were utilized for promotion of exclusive breastfeeding in addition to the routine obstetric services.

Methods

This descriptive study was conducted at a tertiary hospital in Pondicherry, India. Every third primigravida mother admitted in the maternity ward from June to December 2005 was recruited. Among these 144 primigravida mothers, 108 who had a minimum of three antenatal visits ("booked") were included in the study. These 108 mothers were administered a pre-tested semi-structured questionnaire on breastfeeding in the local language, Tamil, within 24 hours of giving birth. Appropriate flash cards with pictures were also used while administering the questionnaire. The awareness among mothers (both "counseled" and "not counseled") regarding health information pertaining to breastfeeding was assessed.

Results

Of the booked mothers, 21% (n = 23) had received some antenatal counseling about breastfeeding while 79% (n = 85) had not received any such counseling. Four percent had undergone breast examination during antenatal visits. Awareness related to breastfeeding among mothers in the "counseled" group was better than those in the "not counseled" group. Even in the "counseled" group, awareness among mothers with regard to correct breastfeeding technique and concept of continuing breastfeeding during illness in the baby was no different from those in the "not counseled" group.

Conclusion

Existing antenatal counseling on breastfeeding is inadequate in the population studied and needs to be strengthened. Informing all pregnant women about the benefits and management of breastfeeding should be a priority during antenatal visits.
Pelvic radiography in ATLS algorithms: A diminishing role?
Authors: Hilty Matthias P , Behrendt Isabelle , Benneker Lorin M , Martinolli Luca , Stoupis Christoforos , Buggy Donald J , Zimmermann Heinz , Exadaktylos Aristomenis K
Published: March 2008

Background

Pelvic x-ray is a routine part of the primary survey of polytraumatized patients according to Advanced Trauma Life Support (ATLS) guidelines. However, pelvic CT is the gold standard imaging technique in the diagnosis of pelvic fractures. This study was conducted to confirm the safety of a modified ATLS algorithm omitting pelvic x-ray in hemodynamically stable polytraumatized patients with clinically stable pelvis in favour of later pelvic examination by CT scan.

Methods

We conducted a retrospective analysis of all polytraumatized patients in our emergency room between 01.07.2004 and 31.01.2006. Inclusion criteria were blunt abdominal trauma, initial hemodynamic stability and a stable pelvis on clinical examination. We excluded patients requiring immediate intervention because of hemodynamic instability.

Results

We reviewed the records of n = 452 polytraumatized patients, of which n = 91 fulfilled inclusion criteria (56% male, mean age = 45 years). The mechanism of trauma included 43% road traffic accidents, 47% falls. In 68/91 (75%) patients, both a pelvic x-ray and a CT examination were performed; the remainder had only pelvic CT. In 6/68 (9%) patients, pelvic fracture was diagnosed by pelvic x-ray. None of these 6 patients was found having a false positive pelvic x-ray, i.e. there was no fracture on pelvic CT scan. In 3/68 (4%) cases a fracture was missed in the pelvic x-ray, but confirmed on CT (false negative on x-ray). None of the diagnosed fractures needed an immediate therapeutic intervention. 5 (56%) were classified type A fractures, and another 4 (44%) B 2.1 in computed tomography (AO classification). One A 2.1 fracture was found in a clinically stable patient who only received CT scan (1/23).

Conclusion

While pelvic x-ray is an integral part of ATLS assessment, this retrospective study suggests that in hemodynamically stable patients with clinically stable pevis, its sensitivity is only 67% and it may safely be omitted in favor of a pelvic CT examination if such is planned in adjunct assessment and available. The results support the safety and utility of our modified ATLS algorithm. A randomized controlled trial using the algorithm can safely be conducted to confirm the results.