bluuebunny/crossref_metadata_2025 huggingface dataset:
Specific Mixing Energy of Cemented Paste Backfill, Part I: Laboratory Determination and Influence on the Consistency
Authors: Dikonda Reagan Kabanga , Mbonimpa Mamert , Belem Tikou
Published: October 2021

Slump determination is widely used to assess the consistency and transportability of fresh cemented paste backfill (CPB). CPB consistency can depend on the mixing procedure for CPB preparation. In this paper, a method was developed to determine the specific mixing energy (SME) that is dissipated during the preparation of CPB mixtures and to analyze its effect on CPB consistency. For this purpose, CPB recipes were prepared using two tailings and the mixing parameters (mixing time and speed and load mass) were successively varied. SME was determined for each mixture using a power meter equipped with an energy recording system mounted on a laboratory Omcan mixer. Slump was also determined for each mixture. A semi-empirical model was then developed to predict SME as a function of the mixing parameters. Results showed that predicted SME compared well with measured SME during CPB preparation. Results also showed that slump increased with increasing SME. The influence of SME on the rheological and mechanical properties of CPB and practical applications are presented in a companion paper.
Facile Preparation and Characteristic Analysis of Sulfated Cellulose Nanofibril via the Pretreatment of Sulfamic Acid-Glycerol Based Deep Eutectic Solvents
Authors: Li Weidong , Xue Yu , He Ming , Yan Jiaqiang , Lucia Lucian A. , Chen Jiachuan , Yu Jinghua , Yang Guihua
Published: October 2021

A deep eutectic solvent (DES) composed of sulfamic acid and glycerol allowed for the sustainable preparation of cellulose nanofibrils (CNF) with simultaneous sulfation. The reaction time and the levels of sulfamic acid demonstrated that fibers could be swelled and sulfated simultaneously by a sulfamic acid-glycerol-based DES and swelling also promoted sulfation with a high degree of substitution (0.12). The DES-pretreated fibers were further nanofibrillated by a grinder producing CNF with diameters from 10 nm to 25 nm. The crystallinity ranged from 53–62%, and CNF maintained the original crystal structure. DES pretreatment facilitated cellulose nano-fibrillation and reduced the energy consumption with a maximum reduction of 35%. The films prepared from polyvinyl alcohol (PVA) and CNF showed good UV resistance ability and mechanical properties. This facile and efficient method provided a more sustainable strategy for the swelling, functionalization and nano-fibrillation of cellulose, expanding its application to UV-blocking materials and related fields.
Crack Length Effect on the Fracture Behavior of Single-Crystals and Bi-Crystals of Aluminum
Authors: Velilla-Díaz Wilmer , Zambrano Habib R.
Published: October 2021

Molecular dynamics simulations of cracked nanocrystals of aluminum were performed in order to investigate the crack length and grain boundary effects. Atomistic models of single-crystals and bi-crystals were built considering 11 different crack lengths. Novel approaches based on fracture mechanics concepts were proposed to predict the crack length effect on single-crystals and bi-crystals. The results showed that the effect of the grain boundary on the fracture resistance was beneficial increasing the fracture toughness almost four times for bi-crystals.
Silver Nanowire Networks: Ways to Enhance Their Physical Properties and Stability
Authors: Bardet Laetitia , Papanastasiou Dorina T. , Crivello Chiara , Akbari Masoud , Resende João , Sekkat Abderrahime , Sanchez-Velasquez Camilo , Rapenne Laetitia , Jiménez Carmen , Muñoz-Rojas David , Denneulin Aurore , Bellet Daniel
Published: October 2021

Silver nanowire (AgNW) networks have been intensively investigated in recent years. Thanks to their attractive physical properties in terms of optical transparency and electrical conductivity, as well as their mechanical performance, AgNW networks are promising transparent electrodes (TE) for several devices, such as solar cells, transparent heaters, touch screens or light-emitting devices. However, morphological instabilities, low adhesion to the substrate, surface roughness and ageing issues may limit their broader use and need to be tackled for a successful performance and long working lifetime. The aim of the present work is to highlight efficient strategies to optimize the physical properties of AgNW networks. In order to situate our work in relation to existing literature, we briefly reported recent studies which investigated physical properties of AgNW networks. First, we investigated the optimization of optical transparency and electrical conductivity by comparing two types of AgNWs with different morphologies, including PVP layer and AgNW dimensions. In addition, their response to thermal treatment was deeply investigated. Then, zinc oxide (ZnO) and tin oxide (SnO2) protective films deposited by Atmospheric Pressure Spatial Atomic Layer Deposition (AP-SALD) were compared for one type of AgNW. We clearly demonstrated that coating AgNW networks with these thin oxide layers is an efficient approach to enhance the morphological stability of AgNWs when subjected to thermal stress. Finally, we discussed the main future challenges linked with AgNW networks optimization processes.
Effects of Lingonberry (Vaccinium vitis-idaea L.) Supplementation on Hepatic Gene Expression in High-Fat Diet Fed Mice
Authors: Ryyti Riitta , Pemmari Antti , Peltola Rainer , Hämäläinen Mari , Moilanen Eeva
Published: October 2021

The prevalence of nonalcoholic fatty liver disease (NAFLD) is growing worldwide in association with Western-style diet and increasing obesity. Lingonberry (Vaccinium vitis-idaea L.) is rich in polyphenols and has been shown to attenuate adverse metabolic changes in obese liver. This paper investigated the effects of lingonberry supplementation on hepatic gene expression in high-fat diet induced obesity in a mouse model. C57BL/6N male mice were fed for six weeks with either a high-fat (HF) or low-fat (LF) diet (46% and 10% energy from fat, respectively) or HF diet supplemented with air-dried lingonberry powder (HF + LGB). HF diet induced a major phenotypic change in the liver, predominantly affecting genes involved in inflammation and in glucose and lipid metabolism. Lingonberry supplementation prevented the effect of HF diet on an array of genes (in total on 263 genes) associated particularly with lipid or glucose metabolic process (such as Mogat1, Plin4, Igfbp2), inflammatory/immune response or cell migration (such as Lcn2, Saa1, Saa2, Cxcl14, Gcp1, S100a10) and cell cycle regulation (such as Cdkn1a, Tubb2a, Tubb6). The present results suggest that lingonberry supplementation prevents HF diet-induced adverse changes in the liver that are known to predispose the development of NAFLD and its comorbidities. The findings encourage carrying out human intervention trials to confirm the results, with the aim of recommending the use of lingonberries as a part of healthy diet against obesity and its hepatic and metabolic comorbidities.
Reply to Landry et al. Findings from Diet Comparison Difficult to Interpret in the Absence of Adherence Assessment. Comment on “Tricò et al. Effects of Low-Carbohydrate versus Mediterranean Diets on Weight Loss, Glucose Metabolism, Insulin Kinetics and β-Cell Function in Morbidly Obese Individuals. Nutrients 2021, 13, 1345”
Authors: Tricò Domenico , Moriconi Diego , Nannipieri Monica
Published: October 2021

We thank Dr. Landry and colleagues [...]
The Role of Short-Chain Fatty Acids in Mediating Very Low-Calorie Ketogenic Diet-Infant Gut Microbiota Relationships and Its Therapeutic Potential in Obesity
Authors: Alsharairi Naser A.
Published: October 2021

As the very low-calorie ketogenic diet (VLCKD) gains increased interest as a therapeutic approach for many diseases, little is known about its therapeutic use in childhood obesity. Indeed, the role of VLCKD during pregnancy and lactation in influencing short chain fatty acid (SCFA)-producing bacteria and the potential mechanisms involved in the protective effects on obesity are still unclear. Infants are characterized by a diverse gut microbiota composition with higher abundance of SCFA-producing bacteria. Maternal VLCKD during pregnancy and lactation stimulates the growth of diverse species of SCFA-producing bacteria, which may induce epigenetic changes in infant obese gene expression and modulate adipose tissue inflammation in obesity. Therefore, this review aims to determine the mechanistic role of SCFAs in mediating VLCKD-infant gut microbiota relationships and its protective effects on obesity.
Yerba Mate—A Long but Current History
Authors: Gawron-Gzella Anna , Chanaj-Kaczmarek Justyna , Cielecka-Piontek Judyta
Published: October 2021

Bearing in mind the growing interest in Yerba Mate, a comprehensive study has been prepared containing the most important aspects and possibilities of its use. The introduction of the work contains the species characteristics of Yerba Mate, as well as information about the origin and places of cultivation. The next part focuses on the analysis of the composition, pointing to purine alkaloids, polyphenols, saponins, and minerals as groups of active compounds responsible for the clinical activity of Yerba Mate. The review of the results of preclinical and clinical studies indicates activity in relation to the stimulating effect, reducing weight by stimulating lipolysis, cardioprotective, anti-diabetic, and anti-inflammatory effects. The information about the action of Yerba Mate is supplemented by the characteristics of its potential toxicity in terms of PAHs content (in particular benzo[α]pyrene) and preparation as a determinant of increased irritation. The current data on the effects of Yerba Mate and the wide safety margin of its use position this raw material as a valuable component of functional food. The growing frequency of consuming Yerba Mate, conditioned by the availability resulting from the globalization of the market and the information provided about it’s the pro-health effects, will position Yerba Mate’s popularity among wider population groups.
Advances in the Detection of Emerging Tree Diseases by Measurements of VOCs and HSPs Gene Expression, Application to Ash Dieback Caused by Hymenoscyphus fraxineus
Authors: Borowik Piotr , Oszako Tomasz , Malewski Tadeusz , Zwierzyńska Zuzanna , Adamowicz Leszek , Tarakowski Rafał , Ślusarski Sławomir , Nowakowska Justyna Anna
Published: October 2021

Ash shoot dieback has now spread throughout Europe. It is caused by an interaction between fungi that attack shoots (Hymenoscyphus fraxineus) and roots (Armillaria spp., in our case Armillaria gallica). While detection of the pathogen is relatively easy when disease symptoms are present, it is virtually impossible when the infestation is latent. Such situations occur in nurseries when seedlings become infected (the spores are carried by the wind several dozen miles). The diseases are masked by pesticides, fertilisers, and adequate irrigation to protect the plants. Root rot that develops in the soil is also difficult to detect. Currently, there is a lack of equipment that can detect root rot pathogens without digging up root systems, which risks damaging trees. For this reason, the use of an electronic nose to detect pathogens in infected tissue of ash trees grown in pots and inoculated with the above fungi was attempted. Disease symptoms were detected in all ash trees exposed to natural infection (via spores) in the forest. The electronic nose was able to detect the pathogens (compared to the control). Detection of the pathogens in seedlings will enable foresters to remove diseased trees and prevent the path from nursery to forest plantations by such selection.
New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of Trichophyton verrucosum in Different Culture Conditions
Authors: Gnat Sebastian , Łagowski Dominik , Nowakiewicz Aneta , Trościańczyk Aleksandra , Dyląg Mariusz
Published: October 2021

Dermatophytes are a group of filamentous fungi infecting skin, hair, and nails that raise great diagnostic difficulties. qRT-PCR is a reliable technique for quantifying gene expression with increasingly frequent use in mycological diagnostics. Knowledge of genes and molecular markers with potential to be used in the identification of dermatophytes is of great importance for the development of this branch of diagnostics. In this article, the suitability of six candidate reference genes (TUBB, ACTB, ADPRF, RPL2, SDHA, and EEF1A1) was investigated for gene expression analysis in the dermatophyte Trichophyton verrucosum, which was cultured in various mycological media that are commonly used in a diagnostic laboratory, i.e., Sabouraud, potato dextrose, and keratin-supplemented MM-Cove. The different culture conditions are extremely important factors for the growth and physiology of dermatophytes. Gene expression stability was evaluated using geNorm, NormFinder, BestKeeper, and RefFinder algorithms. Regarding the stability of expression, SDHA was the most stable housekeeping gene; hence, this gene is recommended for future qRT-PCR studies on T. verrucosum strains. These results allow us to conclude that the SDHA gene can be an additional good candidate as an identification target in the qRT-PCR technique.
Identification of cbiO Gene Critical for Biofilm Formation by MRSA CFSa36 Strain Isolated from Pediatric Patient with Cystic Fibrosis
Authors: Liu Ying , Yang Junshu , Ji Michelle , Phillips James , Wylam Mark , Ji Yinduo
Published: October 2021

The colonization of Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), has a detrimental effect on the respiratory care of pediatric patients with cystic fibrosis (CF). In addition to being resistant to multiple antibiotics, S. aureus also has the ability to form biofilms, which makes the infection more difficult to treat and eradicate. In this study, we examined the ability of S. aureus strains isolated from pediatric patients with CF to form biofilms. We screened a transposon mutant library of MRSA and identified a putative cobalt transporter ATP binding domain (cbiO) that is required for biofilm formation. We discovered that deleting cbiO creating a cbiO null mutant in CFSa36 (an MRSA strain isolated from a patient with cystic fibrosis) significantly hinders the ability of CFSa36 to form biofilm. The complementation of cbiO restored the ability of the cbiO deletion mutant to generate biofilm. Interestingly, we revealed that incorporating extra copper ions to the chemically defined medium (CDM) complemented the function of cbiO for biofilm formation in a dose-dependent manner, while the addition of extra iron ions in CDM enhanced the effect of cbiO null mutation on biofilm formation. In addition, neither the addition of certain extra amounts of copper ions nor iron ions in CDM had an impact on bacterial growth. Taken together, our findings suggest that cbiO mediates biofilm formation by affecting the transportation of copper ions in the MRSA CFSa36 strain. This study provides new insights into the molecular basis of biofilm formation by S. aureus.
Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients
Authors: Skórska Katarzyna Beata , Płaczkowska Sylwia , Prescha Anna , Porębska Irena , Kosacka Monika , Pawełczyk Konrad , Zabłocka-Słowińska Katarzyna
Published: October 2021

Redox status disturbances are known during carcinogenesis and may have influence on patients’ survival. However, the prediction of mortality in lung cancer patients based on serum total SOD activity, and concentrations of its isoforms, has not been studied to date. This prospective cohort study has following aims: (1) to evaluate the disturbances in serum SOD activity and SOD1/2 concentrations; (2) to assess the implications of these alterations with regard to biochemical variables and clinical data, and (3) to investigate the association between serum SOD activity, SOD1/2 concentrations, and all-cause mortality in lung cancer patients. Serum total SOD activity and SOD1, SOD2, albumin, CRP, and ceruloplasmin concentrations were determined in lung cancer patients (n = 190) and control subjects (n = 52). Additionally, patients were characterized in terms of biochemical, clinical, and sociodemographic data. Multiple Cox regression models were used to estimate the association between all-cause death and SOD-related parameters. All-cause mortality in lung cancer was positively associated with serum SOD1 and SOD2 concentrations. Clinical stage III and IV disease was the strongest predictor. The utility of the evaluated parameters in predicting overall survival was demonstrated only for SOD1. Serum SOD1 and SOD2 concentrations were shown to positively affect all-cause mortality in lung cancer patients, but SOD1 seems to be a better predictor than SOD2.
Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
Authors: Valerii Maria Chiara , Turroni Silvia , Ferreri Carla , Zaro Michela , Sansone Anna , Dalpiaz Alessandro , Botti Giada , Ferraro Luca , Spigarelli Renato , Bellocchio Irene , D’Amico Federica , Spisni Enzo
Published: October 2021

Several studies showed that D-Limonene can improve metabolic parameters of obese mice via various mechanisms, including intestinal microbiota modulation. Nevertheless, its effective doses often overcome the acceptable daily intake, rising concerns about toxicity. In this study we administered to C57BL/6 mice for 84 days a food supplement based on D-Limonene, adsorbed on dietary fibers (FLS), not able to reach the bloodstream, to counteract the metabolic effects of a high-fat diet (HFD). Results showed that daily administration of D-Limonene (30 and 60 mg/kg body weight) for 84 days decreased the weight gain of HFD mice. After 84 days we observed a statistically significant difference in weight gain in the group of mice receiving the higher dose of FLS compared to HFD mice (35.24 ± 4.56 g vs. 40.79 ± 3.28 g, p < 0.05). Moreover, FLS at both doses tested was capable of lowering triglyceridemia and also fasting glycemia at the higher dose. Some insights on the relevant fatty acid changes in hepatic tissues were obtained, highlighting the increased polyunsaturated fatty acid (PUFA) levels even at the lowest dose. FLS was also able to positively modulate the gut microbiota and prevent HFD-associated liver steatosis in a dose-dependent manner. These results demonstrate that FLS at these doses can be considered non-toxic and could be an effective tool to counteract diet-induced obesity and ameliorate metabolic profile in mice.
Green Synthesized Silver Nanoparticles Using Tridax Procumbens for Topical Application: Excision Wound Model and Histopathological Studies
Authors: Fatima Farhat , Aldawsari Mohammed F. , Ahmed Mohammed Muqtader , Anwer Md. Khalid , Naz Maimuna , Ansari Mohammad Javed , Hamad Abubaker M. , Zafar Ameeduzzafar , Jafar Mohammed
Published: October 2021

The objective of this study was to synthesize silver nanoparticles from the leaves of Tridax procumbens and develop its topical gels using chitosan to investigate the wound healing efficacy concomitant with the histopathological study. Green synthesized silver nanoparticles (AgNPs) were prepared by reacting silver nitrate (0.3 M) with leaf extract and characterized by particle analysis, FTIR, XRD, SEM, BET, and TGA. The results revealed formed AgNPs were nano-sized (138 ± 2.1 nm), monodispersed (PDI: 0.460 ± 0.3), inter-particle repulsion (zeta: −20.4 ± 5.20 mV), stabilized, crystalline and, spherical with size ranging from 80–100 nm as per SEM micro photos. The BET analysis of AgNPs presents the surface area (12.861 m2/g), pore volume (0.037 cc/g), and pore radius (24.50 nm).TGA results show a loss of 13.39% up to 300 °C. The topical formulation was developed by loading AgNPs in chitosan-based gels, evaluated by pH, thermal cycling, centrifugal, and spreadability tests. AgNPs chitosan gels results showed skin compatibility, higher stability, and spreading ability. The maximum antibacterial zone of inhibition was found to be 25 ± 0.98 mm for bacillus subtitles and 30 ± 1.99 mm for Klebsiella pneumoniae, respectively. Nanosilver-containing gel also showed excellent compatibility with erythrocytes. Excision wound model was used to assess the wound healing property of the developed AgNP gels, the results of which indicated a significantly progressive healing process in test-group of animals treated with chitosan-based gels containing AgNPs. A histopathological study further confirmed the almost normal skin structure of treated animal tissue compared to standard and negative control. Thus, green synthesized AgNPs loaded chitosan-based topical gel can potentially be used for wound healing application.
Advances of Nanomedicine in Radiotherapy
Authors: Liu Wei , Chen Bo , Zheng Haocheng , Xing Yun , Chen Guiyuan , Zhou Peijie , Qian Liting , Min Yuanzeng
Published: October 2021

Radiotherapy (RT) remains one of the current main treatment strategies for many types of cancer. However, how to improve RT efficiency while reducing its side effects is still a large challenge to be overcome. Advancements in nanomedicine have provided many effective approaches for radiosensitization. Metal nanoparticles (NPs) such as platinum-based or hafnium-based NPs are proved to be ideal radiosensitizers because of their unique physicochemical properties and high X-ray absorption efficiency. With nanoparticles, such as liposomes, bovine serum albumin, and polymers, the radiosensitizing drugs can be promoted to reach the tumor sites, thereby enhancing anti-tumor responses. Nowadays, the combination of some NPs and RT have been applied to clinical treatment for many types of cancer, including breast cancer. Here, as well as reviewing recent studies on radiotherapy combined with inorganic, organic, and biomimetic nanomaterials for oncology, we analyzed the underlying mechanisms of NPs radiosensitization, which may contribute to exploring new directions for the clinical translation of nanoparticle-based radiosensitizers.
Exceptional Points through Variation of Distances between Four Coaxial Dielectric Disks
Authors: Pichugin Konstantin , Sadreev Almas , Bulgakov Evgeny
Published: October 2021

By variation of a refractive index and aspect ratio of the isolated disk, we achieved exceptional points (EPs) at which the resonant frequencies and resonant modes coalesce. However, in practice, that kind of variation presents a technological problem. We considered the method to avoid the problem by substitution of two disk’s dimers. In each dimer, variation of the distance between disks was equivalent to a variation of the aspect ratio of the dimer. Moreover, the variation of the distance between dimers provides the second parameter that gives rise to a vast number of EPs. We recovered the initial resonant eigenmode by encircling multiple EPs two, three, and four times in the two-dimensional parametric space of distances.
Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
Authors: Maklygina Yuliya , Romanishkin Igor , Skobeltsin Aleksej , Farrakhova Dina , Loschenov Victor
Published: October 2021

In this work, a new approach was tested to assess the cellular composition of tissues by time-resolved methods of fluorescence analysis of exogenous and endogenous fluorophores. First of all, the differences in fluorescence kinetics of endogenous fluorophores (coenzymes NADH and FAD) in tumour and immunocompetent cells were determined. After that, differences in fluorescence kinetics of photosensitizer 5 ALA-induced protoporphyrin IX were established due to its different metabolism in cells of different phenotypes. Kinetics of photoluminescence of NADH and FAD coenzymes as well as photosensitizer were studied by means of two different methods: time-resolved spectroscopy based on a streak-camera and fibre optic neuroscopy, which served to perform process monitoring and regular fluorescence diagnosis of the probed region. Time-resolved fluorescence microscopy (FLIM) was used as a control technique. Time-resolved spectroscopic fluorescence lifetime analysis was performed on sexually mature female rats induced with glioma C6 brain tumour under in vivo conditions; thus, under conditions where the immune system actively intervenes in the process of oncogenesis. In this regard, the aim of the study was to recognize the cellular composition of the brain tumour tissue, namely the ratio of cancer and immunocompetent cells and their mutual localization. Understanding the role of the immune system thus provides new ways and approaches for further diagnosis and therapy, making tumour-associated immune cells a prime target for modern therapies.
Nitrogen Source Preference and Growth Carbon Costs of Leucaena leucocephala (Lam.) de Wit Saplings in South African Grassland Soils
Authors: Sithole Nonkululeko , Tsvuura Zivanai , Kirkman Kevin , Magadlela Anathi
Published: October 2021

Leucaena leucocephala (Fabaceae) is native to Central America and has invaded many climatic regions of the tropics. In South Africa, the species is categorized as an emerging or incipient weed used as fodder, timber, firewood and in erosion control on degraded habitats. The species is common along the eastern subtropical regions of KwaZulu-Natal (KZN) Province, where it invades grasslands, savannas and edges of forests. Soils of these ecosystems are characterized as nutrient deficient and acidic. Using a pot trial, we determined the effects of the nutrient addition treatments on microbial symbiosis, N nutrition and biomass accumulation of L. leucocephala under greenhouse conditions. After 180 days of growth, plants were harvested, and their utilization of N derived from the atmosphere and from the soil was quantified through determination of δ15N values. L. leucocephala maintained growth and N nutrition by relying on both atmospheric- and soil-derived N across all soil treatments. The NDFA was significantly higher in high P (N1 + P, N2 + P and N3 + P) soils. L. leucocephala was able to nodulate with intermediate and fast-growing strains from the Mesorhizobium and Rhizobium genus in N2 + P grown plants. This shows that L. leucocephala possesses traits that are successful in acquiring nutrients, especially in nutrient limited conditions, by establishing plant symbiosis with multiple bacteria and relying on extracting N from the soil and from the atmosphere through the symbiosis.
Artemisia annua Growing Wild in Romania—A Metabolite Profile Approach to Target a Drug Delivery System Based on Magnetite Nanoparticles
Authors: Segneanu Adina-Elena , Marin Catalin Nicolae , Ghirlea Ioan Ovidiu-Florin , Feier Catalin Vladut Ionut , Muntean Cornelia , Grozescu Ioan
Published: October 2021

The metabolites profile of a plant is greatly influenced by geographical factors and the ecological environment. Various studies focused on artemisinin and its derivates for their antiparasitic and antitumoral effects. However, after the isolation and purification stage, their pharmaceutical potential is limited due to their low bioavailability, permeability and lifetime. The antibacterial activity of essential oils has been another topic of interest for many studies on this plant. Nevertheless, only a few studies investigate other metabolites in Artemisia annua. Considering that secondary metabolites act synergistically in a plant, the existence of other metabolites with antitumor and high immunomodulating activity is even more important. Novel nano-carrier systems obtained by loading herbs into magnetic nanoparticles ensures the increase in the antitumor effect, but also, overcoming the barriers related to permeability, localization. This study reported the first complete metabolic profile from wild grown Romanian Artemisia annua. A total of 103 metabolites were identified under mass spectra (MS) positive mode from 13 secondary metabolite categories: amino acids, terpenoids, steroids, coumarins, flavonoids, organic acids, fatty acids, phenolic acids, carbohydrates, glycosides, aldehydes, hydrocarbons, etc. In addition, the biological activity of each class of metabolites was discussed. We further developed a simple and inexpensive nano-carrier system with the intention to capitalize on the beneficial properties of both components. Evaluation of the nano-carrier system’s morpho-structural and magnetic properties was performed.
Effects of a Dominant Species on the Functional Diversity of Coexisting Species in Temperate Deciduous Understorey
Authors: Kaushik Krishan , Bricca Alessandro , Mugnai Michele , Viciani Daniele , Rudolf Kinga , Somfalvi-Tóth Katalin , Morschhauser Tamás
Published: October 2021

The herb layer plays a significant role in maintaining forest functions, and its community composition is determined by various abiotic factors and biotic interactions. This study attempted to investigate the interspecific plant–plant biotic interactions using a functional traits approach. Specifically, the effects of a dominant species coverage on the functional diversity of coexisting species in the temperate forest understory were studied. Species coverage and soil moisture data were collected using a 1 m2 quadrat couplet (2 × 1 m2) from six sites alongside a 20 m linear transect encompassing a cover gradient of Allium ursinum in southwest Hungary. Major plant functional dimensions i.e., aboveground, and clonal functional traits were considered. Linear and nonlinear mixed models to quantify the effects of biotic interaction on the functional diversity of every single trait and multiple traits were employed. Both aboveground traits and clonal traits of persistent clonal growth organs responded positively to the A. ursinum L., cover gradient. The coexistence of understory species in the presence of a monodominant species seems to be mainly influenced by aboveground traits as compared to the clonal traits suggesting, a role of niche differentiation. The consistent impact of A. ursinum coverage on coexisting species dynamics highlights a need for similar in-depth studies in various forest settings.
Enhanced Knittability of Paper Yarn from the Swedish Forest by Using Textile Finishing Materials
Authors: Syrén Felicia , Andersson Drugge Gabriella , Peterson Joel , Kadi Nawar
Published: October 2021

Friction between Swedish paper yarn and needles is a limiting factor that—together with the low yarn flexibility—is hindering the knitting and use of paper yarn as a sustainable textile material. To enhance the knittability, paper yarn was coated with textile finishing materials. The effect of six different textile finishing materials used for textiles processing (three different silicone-based, wax, glycerol, and soap) was evaluated. The treatment evaluation was done by determination of the friction coefficient, tensile testing, and knitting. The friction coefficient was determined by an adaption from the ASTM D3108-07 Standard Test Method for Coefficient of Friction, Yarn to Solid Material. The adaption meant using a specially designed rig, making it possible to simulate the yarn/needle friction during the knitting process and use a tensile testing machine to determine the friction coefficient. Through using the same angle for yarn movement during the knitting process in this adaptation, the effect of the flexibility of paper on the friction coefficient is integrated. Tensile testing was performed using a Tensolab 2512A/2512C electromechanical tensile tester, and knitting tests were performed using a Stoll CMS 822 HP knit and wear flat knitting machine with the E5.2 gauge. The results show that knittability is better for the yarns with lower coefficients of friction and can also be enhanced by spraying with regular water. The tensile properties of the yarn is degraded by the treatments. The wax- and soap-treated yarns were most challenging to knit. The silicone-based and glycerol-treated yarns showed enhanced knittability, where the glycerol treatment results in more protruding fibers compared to the other treatments. All treatments reduced the roughness in the feel of the knit. The results indicate that the Swedish paper yarn can be a future sustainable complement to polyester and cotton.
Ultrafiltration of α-Lactalbumin Protein: Acquaintance of the Filtration Performance by Membrane Structure and Surface Alteration
Authors: Arahman Nasrul , Rosnelly Cut Meurah , Yusni Yusni , Fahrina Afrillia , Silmina Silmina , Ambarita Aulia Chintia , Bilad Muhammad Roil , Gunawan Poernomo , Rajabzadeh Saeid , Takagi Ryosuke , Matsuyama Hideto , Aziz Muhammad
Published: October 2021

α-Lactalbumin is an essential protein with multiple roles in physiological and the nutritional functionalities, such as diabetic prevention, blood pressure stabilization, and cancer cell inhibition. In the present work, polyethersulfone (PES)-based membranes were developed by incorporating Pluronic F127 and carbon nanotubes with single- and multi-walled dimensions (Sw-Cnts and Mw-Cnts) as additives. The resulting membranes were evaluated for use in the filtration of α-lactalbumin protein solution. Four series of membranes, including PES pristine membrane, were fabricated via the phase inversion process. The characteristics of the membrane samples were analyzed in terms of morphology, membrane surface hydrophilicity and roughness, and surface chemistry. The characterization results show that the incorporation of additive increased the surface wettability by reducing the surface water contact angle from 80.4° to 64.1° by adding F127 and Mw-Cnt additives. The highest pure water permeability of 135 L/(m2·h·bar) was also exhibited by the PES/F127/Mw-Cnt membrane. The performance of the modified membranes was clearly better than the pristine PSF for α-lactalbumin solution filtration. The permeability of α-lactalbumin solution increased from 9.0 L/(m2·h·bar) for the pristine PES membrane to 10.5, 11.0 and 11.5 L/(m2·h·bar) for membranes loaded with Pluronic F127, Sw-Cnts, and Mw-Cnts, respectively. Those increments corresponded to 17, 22, and 28%. Such increments could be achieved without altering the α-lactalbumin rejections of 80%. Remarkably, the rejection for the membrane loaded with Sw-Cnts even increased to 89%.
Understanding Variations in the Tracking and Erosion Performance of HTV-SR-Based Composites due to AC-Stressed Aging
Authors: Ullah Rahmat , Akbar Muhammad , Ullah Nasim , Otaibi Sattam Al , Althobaiti Ahmed
Published: October 2021

Among the polymeric family, high-temperature-vulcanized silicone rubber (HTV-SR) is the most deployed material for high voltage insulation applications. However, in an outdoor environment, due to contamination and wetting-induced dry band arcing, consequently SR experiences surface tracking and erosion. From a practical standpoint, the tracking and erosion performance under multi-stress aging is required to be known. It is in that context that the present study was undertaken to measure and analyze the effect of multi-stress aging on tracking and erosion performance. Composite samples of SR having different filler concentrations of silica and alumina trihydroxide (ATH) were aged in a multi-stress chamber for a period of 5000 h, and after that their electrical tracking performance was studied. Simultaneously, unaged samples were also exposed to tracking test for comparison. To conduct this test, the inclined plane testing technique was used in accordance with IEC-60587. All samples exposed to tracking test were analyzed using different diagnostic and measuring techniques involving surface leakage current measurement, Fourier transform infrared spectroscopy (FTIR), thermal stability and hydrophobicity classification. Experimental results shown that the tracking lifetime increased through incorporation of silica and ATH fillers in the SR. Amongst all test samples, two samples designated as filled with 2% nano silica and 20% micro silica/ATH exhibited greater resistance to tracking. This was attributed to the optimum loading as well as better dispersion of the fillers in the polymer matrix. The presence of nano-silica enhanced time-to-tracking failure, owing to both improved thermal stability and enhanced shielding effect on the surface of nanocomposite insulators.
A Study of Compressibility, Compactability and Mucoadhesivity of Tableting Materials for Matrix Systems Based on Chitosan
Authors: Muzikova Jitka , Snejdrova Eva , Martiska Juraj , Doubkova Bara , Veris Andrea
Published: October 2021

The objective of the present research is to evaluate directly compressible chitosan-based tableting materials for the formulation of mucoadhesive matrix tablets intended for targeted drug release to distal segments of the GIT. The influence of sodium alginate, hypromellose, and silicified microcrystalline cellulose (P90) on compressibility, compactability and lubricant sensitivity ratio was tested. Furthermore, the rheological properties of the hydrated surface layer of the matrix tablets and the mucoadhesion to a mucin substrate were analysed. Compressibility was evaluated using the energy profile of the compression process, compactability by means of the tensile strength of tablets, and lubricant sensitivity ratio was calculated to assess the sensitivity to lubricant. Addition of P90 to chitosan improved compressibility, which is demonstrated by the increase in the energy of plastic deformation and the higher tensile strength of tablets. P90 also significantly reduced the high lubricant sensitivity of chitosan. Presence of retarding components led to a decrease in Emax. All tested matrix tablets revealed a good mucoadhesion without a negative effect of P90 content. The viscosity of a gel layer on the surface of matrix tablets containing hypromellose was higher compared to those with sodium alginate. This was not reflected in the adhesive strength of the tablets. The formulated tableting materials combining chitosan and P90 are a suitable matrix for incorporation of an active ingredient, whose delayed release in the intestine can be achieved by the functionality of the chitosan-sodium alginate complex.
Effects of Coefficient of Thermal Expansion and Moisture Absorption on the Dimensional Accuracy of Carbon-Reinforced 3D Printed Parts
Authors: Faust Jessica L. , Kelly Peter G. , Jones Bruce D. , Roy-Mayhew Joseph D.
Published: October 2021

Environmental effects—temperature and moisture—on 3D printed part dimensional accuracy are explored. The coefficient of thermal expansion of four different nylon materials was determined for XY and ZX print orientations, with 0°, 45°/−45°, and 90° infill patterns. Unreinforced nylon exhibited a thermal expansion coefficient of the same order regardless of condition (from 11.4 to 17.5 × 10−5 1/°C), while nylons reinforced with discontinuous carbon fiber were highly anisotropic, for instance exhibiting 2.2 × 10−5 1/°C in the flow direction (0° infill angle) and 24.8 × 10−5 1/°C in the ZX orientation. The temperature profile of a part during printing is shown, demonstrating a build steady state temperature of ~ 35 °C. The effect of moisture uptake by the part was also explored, with dimensional changes of ~0.5–1.5% seen depending on feature, with height expanding the most. The effects of moisture were significantly reduced for large flat parts with the inclusion of continuous fiber reinforcement throughout the part.
Multiobjective Optimization Based on “Distance-to-Target” Approach of Membrane Units for Separation of CO2/CH4
Authors: Abejón Ricardo , Casado-Coterillo Clara , Garea Aurora
Published: October 2021

The effective separation of CO2 and CH4 mixtures is essential for many applications, such as biogas upgrading, natural gas sweetening or enhanced oil recovery. Membrane separations can contribute greatly in these tasks, and innovative membrane materials are being developed for this gas separation. The aim of this work is the evaluation of the potential of two types of highly CO2-permeable membranes (modified commercial polydimethylsiloxane and non-commercial ionic liquid–chitosan composite membranes) whose selective layers possess different hydrophobic and hydrophilic characteristics for the separation of CO2/CH4 mixtures. The study of the technical performance of the selected membranes can provide a better understanding of their potentiality. The optimization of the performance of hollow fiber modules for both types of membranes was carried out by a “distance-to-target” approach that considered multiple objectives related to the purities and recovery of both gases. The results demonstrated that the ionic liquid–chitosan composite membranes improved the performance of other innovative membranes, with purity and recovery percentage values of 86 and 95%, respectively, for CO2 in the permeate stream, and 97 and 92% for CH4 in the retentate stream. The developed multiobjective optimization allowed for the determination of the optimal process design and performance parameters, such as the membrane area, pressure ratio and stage cut required to achieve maximum values for component separation in terms of purity and recovery. Since the purities and recoveries obtained were not enough to fulfill the requirements imposed on CO2 and CH4 streams to be directly valorized, the design of more complex multi-stage separation systems was also proposed by the application of this optimization methodology, which is considered as a useful tool to advance the implementation of the membrane separation processes.
Croton cajucara Essential Oil Nanoemulsion and Its Antifungal Activities
Authors: Azevedo Mariana M. B. , Almeida Catia A. , Chaves Francisco C. M. , Ricci-Júnior Eduardo , Garcia Andreza R. , Rodrigues Igor A. , Alviano Celuta S. , Alviano Daniela S.
Published: October 2021

The purpose of this study was to develop a stable nanoemulsion (NE) containing Croton cajucara 7-hydroxycalamenene-rich essential oil (NECC) with antifungal activity. The NECCs were prepared using an ultrasonic processor with Pluronic® F-127 as the aqueous phase. In order to evaluate the NECCs, the droplet size, polydispersity index (PdI), percentage of emulsification, and pH were determined along with a stability study. The NECC selected for the study had 15% surfactant, showed 100% emulsification, Pdl of 0.249, neutral pH, droplet diameters of about 40 nm, and remained stable over 150 days at room temperature. In addition, the NECC activity against some species of Zygomycetes and Candida, as well as the potential to inhibit fungal extracellular proteases, were assessed, and, finally, the hemolytic activity was evaluated. The best NECC antifungal activities were against Mucorramosissimus (Minimal inhibitory concentration (MIC) = 12.2 μg/mL) and Candida albicans (MIC = 25.6 μg/mL). The highest extracellular protease activities of M. ramosissimus and C. albicans were detected at pH 3 and 4, respectively, which were totally inhibited after NECC treatment. The NECC showed no hemolytic effect at the highest concentration tested (2 mg/mL).
The Dark Side of Platinum Based Cytostatic Drugs: From Detection to Removal
Authors: Roque-Diaz Yessica , Sanadar Martina , Han Dong , López-Mesas Montserrat , Valiente Manuel , Tolazzi Marilena , Melchior Andrea , Veclani Daniele
Published: October 2021

The uncontrolled release of pharmaceutical drugs into the environment raised serious concerns in the last decades as they can potentially exert adverse effects on living organisms even at the low concentrations at which they are typically found. Among them, platinum based cytostatic drugs (Pt CDs) are among the most used drugs in cancer treatments which are administered via intravenous infusion and released partially intact or as transformation products. In this review, the studies on environmental occurrence, transformation, potential ecotoxicity, and possible treatment for the removal of platinum cytostatic compounds are revised. The analysis of the literature highlighted the generally low total platinum concentration values (from a few tens of ng L−1 to a few hundred μg L−1) found in hospital effluents. Additionally, several studies highlighted how hospitals are sources of a minor fraction of the total Pt CDs found in the environment due to the slow excretion rate which is longer than the usual treatment durations. Only some data about the impact of the exposure to low levels of Pt CDs on the health of flora and fauna are present in literature. In some cases, adverse effects have been shown to occur in living organisms, even at low concentrations. Further ecotoxicity data are needed to support or exclude their chronic effects on the ecosystem. Finally, fundamental understanding is required on the platinum drugs removal by MBR, AOPs, technologies, and adsorption.
Biogas Production by Pilot-Scale Anaerobic Co-Digestion and Life Cycle Assessment Using a Real Scale Scenario: Independent Parameters and Co-Substrates Influence
Authors: Mosquera Jhessica , Rangel Carol , Thomas Jogy , Santis Angelica , Acevedo Paola , Cabeza Ivan
Published: October 2021

This study evaluates the performance of different agricultural by-products to identify the potential effect of independent variables, using as the dependent variable the biogas production. A Box–Behnken experimental design was carried out in a pilot-scale plant of four stirred stainless-steel digesters under mesophilic semi-continuous digestion. The results obtained support the creation of a technical framework to scale up the process and further evaluation of the potential environmental impacts through life cycle assessment (LCA) methodology. A stable behaviour was achieved in 12 of the 13 experiments proposed. The highest value of daily biogas production was 2200.15 mL day−1 with a stabilization time of 14 days, an organic loading rate of 4 g VS feed daily, low C/N ratio and a 1:1 relation of nitrogen providers. The concentrations of CH4 remained stable after the production stabilization and an average biogas composition of 60.6% CH4, 40.1% CO2 and 0.3% O2 was obtained for the conditions mentioned above. Therefore, the real scale plant was estimated to manage 2.67 tonnes of residual biomass per day, generating 369.69 kWh day−1 of electricity. The LCA analysis confirms that the co-digestion process evaluated is a feasible and environmentally sustainable option for the diversification of the Colombian energy matrix and the development of the agro-industrial sector.
Improvement of Perception Layer Routing Protocol with Static Nodes in IoT-Based Microgrids
Authors: Yang Jun , Yu Chang , Hu Zijian
Published: October 2021

Aiming at the problem of uneven clustering and the unreasonable energy consumption of LEACH protocol in the perception layer of IoT-based microgrids of static nodes; in this paper, we propose a stationary-node energy-based routing protocol (SERP). First, we select a dynamic cluster radius for clustering to meet the actual needs of the network during clustering. Then, to solve the problem that the number of cluster heads is difficult to determine, a dynamic optimal cluster head ratio is adopted. The dynamic optimal cluster head ratio can be obtained by minimizing the total energy consumption of cluster formation and the stable transmission phase, which can improve the efficiency of network transmission. Finally, by setting the residual energy factor and distance factor to improve the calculation of the cluster head election threshold, the energy load of the network is more uniform, and the location of the cluster head is more reasonable. Compared with the LEACH protocol and the HEED protocol, the simulation results show that the SERP protocol can effectively prolong the lifetime of the whole network.