bluuebunny/crossref_metadata_2025 huggingface dataset:
Female Warriors, Social Injustice and the Transformational Force of Anger in Jaye Wells' Sabina Kane Series
Authors: Golubov Nattie
Published: January 2024

Urban fantasy heroines tend to be highly trained supernatural or magical beings who unapologetically use violence to achieve their goals. Their stories, often plotted as revenge narratives, are in many cases driven by anger, a key force of good in the quest for justice. Heroines are transformed into protectors when their personal trajectories, often motivated by complicated family bonds, become entangled with the fate of specific cities. Rereading Jaye Wells’ Sabina Kane series in the context of recent feminist reinterpretations of women’s anger, I will argue that anger is a source of social responsibility, thus resolving the apparent contradiction between caring and violence, and can become a legitimate affect in romance fiction. I will explain how, in Wells series, the female questor achieves the transition from lone wolf to caring warrior without sacrificing her capacity to expertly wield extreme violence or deny her rage.
Immune, RNA, and Neurocognitive Genetic Networks in Bipolar Disorder Subtypes: A Transcriptomic Meta-Analysis
Authors: Jang Tyler , Kaul Marcus
Published: January 2024

Background: Little is known about the pathogenesis of Bipolar Disorder, and even less is known about the genetic differences between its subtypes. Bipolar Disorder is classified into different subtypes, which present different symptoms and lifetime courses. While genetic studies have been conducted in Bipolar Disorder, most examined the gene expression of only Bipolar Disorder Type 1. Studies that include Bipolar Disorder Type 1 and Bipolar Disorder Type 2 often fail to differentiate them into separate conditions. Few large transcriptomic meta-analyses in Bipolar Disorder have been conducted to identify genetic pathways. Thus, using publicly available data sets we aim here to uncover significant differential gene expression that allows distinguishing Type 1 and Type 2 Bipolar Disorders, as well as find patterns in Bipolar Disorder as a whole.
Methods: We analyze 17 different gene expression data sets from different tissue in Bipolar Disorder using GEO2R and manual analysis, of which 15 contained significant differential gene expression results. We use STRING and Cytoscape to examine Gene Ontology to find significantly affected genetic pathways. We identify hub genes using cytoHubba, a plugin in Cytoscape. We find genes common to data sets of the same material or subtype.
Results: 12 out of 15 data sets are enriched for immune system and RNA related pathways. 9 out of 15 data sets are enriched for neurocognitive and metal ion related GO terms. Analysis of Bipolar Disorder Type 1 vs Bipolar Disorder Type 2 revealed most differentially expressed genes were related to immune function, especially cytokines. Terms related to synaptic signaling and neurotransmitter secretion were found in down-regulated GO terms while terms related to neuron apoptosis and death were up-regulated. We identify the gene SNCA as a potential biomarker for overall Bipolar Disorder diagnosis due to its prevalence in our data sets.
Conclusions: The immune system and RNA related pathways are significantly enriched across the Bipolar Disorder data sets. The role of these pathways is likely more critically important to the function of Bipolar Disorder than currently understood. Further studies should clearly label the subtype of Bipolar Disorder used in their research and more effort needs to be undertaken to collect samples from Cyclothymic Disorder and Bipolar Disorder Type 2.
Atmospheric Emissions Assessment for LNG Carriers: A State-of-the-Art Semi-Empirical Methodology
Authors: Gutiérrez Carlos González , Martinez Alvaro Herrero , de Navamuel Emma Diaz Ruiz , Piris Andres Ortega , Rojo Beatriz Blanco
Published: January 2024

This research establishes an extensive set of algorithms for the precise evaluation of fuel consumption across a range of engine types and systems onboard LNG carriers (LNGCs). The principal objective of this research is the development of a modern, universally applicable, and refined methodology tailored to LNGCs. This methodology encompasses the comprehensive calculation of both fuel consumption and atmospheric emissions, accommodating the variability in engine configurations. Furthermore, the integration of empirical data derived from 73 operational LNGCs yields a meticulously crafted operational profile. This consequential outcome of our study carries significant implications for addressing forthcoming modelling complexities specific to LNGCs.
Bayesian Network-based Mendelian Randomization for Variant Prioritization and Phenotypic Causal Inference
Authors: Sun Jianle , Zhou Jie , Gong Yuqiao , Pang Chongchen , Ma Yanran , Zhao Jian , Yu Zhangsheng , Zhang Yue
Published: January 2024

Mendelian randomization is a powerful method for for inferring causal relationships. However, obtaining suitable genetic instrumental variables is often challenging due to gene interaction, linkage, and pleiotropy. We propose Bayesian Network-based Mendelian Randomization (BNMR), a Bayesian causal learning and inference framework using individual-level data. BNMR employs the random graph forest, an ensemble Bayesian network structural learning process, to prioritize candidate genetic variants and select appropriate instrumental variables, and then obtains a pleiotropy-robust estimate by incorporating a shrinkage prior in the Bayesian framework. Simulations demonstrate BNMR can efficiently reduce the false positive discoveries in variant selection, and outperforms existing MR methods in terms of accuracy and statistical power in effect estimation. With application to the UK Biobank, BNMR exhibits its capacity in handling modern genomic data, and reveals the causal relationships from hematological traits to blood pressures and psychiatric disorders. Its effectiveness in handling complex genetic structures and modern genomic data highlight the potential to facilitate real-world evidence studies, making it a promising tool for advancing our understanding of causal mechanisms.
Trade openness, human capital, natural resource and carbon emission nexus: A CS-ARDL assessment for Central Asian Economies
Authors: Feng Meng Qing , Morake Otsile , Sampene Agyemang Kwasi , Agyeman Fredrick Oteng
Published: January 2024

There is a call for global efforts to preserve the ecological systems that can sustain economies and people’s lives. However, carbon emission (CEM) poses a danger to the sustainability of humanity and ecological systems. This analysis looked into the influence of energy use (ERU), human capital (HCI), trade openness (TOP, natural resource (NRR), population, and economic growth (ENG) on CEM. The paper gathered panel data ranging from 1990 to 2020 from the Central Asia region. The CS-ARDL was applied to establish the long-term interaction among the indicators. The paper findings indicated the presence of the Environmental Kuznets Curve (EKC) in the Central Asia regions. Also, the empirical evidence highlighted that energy use, natural resources, and trade openness cause higher levels of CEM. However, the research verified that CEM can be improved through human capital and urban population growth. The study also found that HCI moderates the interaction between NRR and CEM. The causality assessment indicated a one-way interplay between ENG, ERU, NRR, and CEM. The study proposes that in order to support ecological stability in these regions, policy-makers should concentrate on developing human capital, investing in renewable energy sources, and utilizing contemporary technologies to harness natural resources in the economies of Central Asia.
Associations between Recurrent COVID-19, Attention, and Mental Health: A Longitudinal Study
Authors: Khodami Mohammad Ahsan , Hosseini Seyed Mohammad , Bagheri Seyran , Kireeva Sofia
Published: January 2024

This two-part longitudinal study examined the impacts of initial and recurrent COVID-19 diagnosis on negative affect (Depression, Anxiety, and Stress) and attention control in undergraduate students during pandemic lockdowns. Study 1 found that first-time diagnosis was associated with significantly higher depression, anxiety, and stress but did not affect attention control. Study 2 showed recurrent diagnosis further worsened mental health outcomes yet was linked to enhanced attentional abilities, contrasting typical condition declines. Within-subjects analysis demonstrated a worsening effect but improved attention control from first to second-time points among repeatedly diagnosed participants. Overall, both initial and recurrent diagnoses severely impact psychological well-being with cumulative mental health tolls, underscoring the need to prioritize supporting mental health. However, attentional resilience emerges, potentially reflecting an adaptive coping response. Continued research tracking patients across multiple infections can clarify relationships between worsening emotions and improved attention, informing interventions to address the multifaceted health impacts of COVID-19 reinfection.
Magma chamber longevity on Mars and its controls on crustal structure and composition
Authors: Chatterjee Arka , Huber Christian , III James Head , Bachmann Olivier
Published: January 2024

In volcanically active planetary bodies, the depths and longevity of crustal magma storage critically control eruptibility and crustal composition. A paucity of observations has challenged our understanding of the development of crustal magma storage systems in Mars and its role behind the lack of evolved compositions. Here, we use numerical modelling, together with recent results from the InSight mission, to study the evolution of crustal magma chambers on Mars and conditions that promote their growth and eruptibility. We find that the Martian crust can be divided, by depth, into three major domains. At depths ≤15km (~1.5kbar), trapped magma pods are small, short-lived, with high diking potential, hindering the production of evolved compositions. While depths >25km (~2.5kbar) can host long-lived magma chambers, 15-25km (~2 ± 0.5kbar) marks a transition where magma chambers could grow while expelling magma. Interestingly, this narrow depth window overlaps with the depth of an intra-crustal discontinuity reported by InSight, suggesting a possible magmatic origin for the discontinuity. We further show that crustal rheology strongly controls this transition depth. Our results also support the possibility of deep-seated magmatism underneath the seismically active Cerberus Fossae, suggesting that magmatism continues to play a major role in shaping the Martian crust.
Bridging the Gap in Rural Emergency Care: A Scoping Review of Living Lab Hospitals and their Potential in Rural Healthcare
Authors: JOYAL ROSE , Tounkara Fatoumata Korika , Singhroy Diane , Fleet Richard
Published: January 2024

Background. Rural Canadian hospitals are increasingly vulnerable to service reductions and emergency department closures, compromising the emergency care access for the 6 million residents (20% of the population) living in these areas. Current standards for rural emergency care in Canada are outdated, necessitating innovative, locally adapted solutions. The Living Lab concept, a collaborative, real-world innovation ecosystem involving patients, citizens, healthcare professionals, learners and researchers, may address this gap. In 2020, we initiated a rural Living Lab hospital project in Charlevoix, Québec, where no prior studies on such hospitals existed. Our research aims to assess the current state of literature on rural Living Lab hospitals.
Objectives. Our specific goal was to examine and characterize the existing research on Living Lab Hospitals in rural settings while precisely identifying areas where further research is required.
Methodology. Our scoping review was conducted through a structured five-stage process: [1] problem formulation, [2] literature search, across five databases: PubMed, MEDLINE, EMBASE, CINAHL, and Web of Sciences, [3] data evaluation and analysis, [4] data collection and summary, and [5] presentation.
Results. A total of 212 studies from Embase, 134 from MEDLINE, 91 from CINAHL, 10 from Web of Science, and 4 from PubMed were initially part of our analysis. After eliminating 169 duplicate entries, our team scrutinized 282 titles and abstracts. Given our primary aim of identifying living labs based in rural hospital settings, only our original publication, “A Canadian Rural Living Lab Hospital: Implementing solutions for improving rural emergency care”, was found.
Conclusion: This scoping review indicates a stark research deficit on Living Lab hospitals in rural contexts, with only one relevant study identified. This highlights a critical need for further investigation into the adoption and impact of Living Lab models in rural healthcare. Given the risks faced by rural emergency services, it is vital to prioritize research in this area to improve and innovate care for vulnerable populations.
Quality Assessment of Biomass Pellets Available on the Market; Example From Poland
Authors: Drobniak Agnieszka , Jelonek Zbigniew , Mastalerz Maria , Jelonek Iwona , Widziewicz-Rzońca Kamila
Published: January 2024

This study evaluates the quality of 30 biomass pellets sold for residential use in Poland. It provides data on their physical, chemical, and petrographic properties and compares them to existing standards and the information provided by the fuel producers. The results reveal considerable variations in the quality of the pellets and show that some of the purchased samples are not within the DINplus and/or ENplus certification thresholds. Among all 30 purchased samples, only one passes the quality thresholds set by the US-PL BIO, a newly established quality certification in Poland that combines quality assessment following DINplus with optical microscopy analysis. The primary issues causing a decrease in pellet quality include elevated ash and fines content, compromised mechanical durability, too low ash melting temperature, and additions of undesired additions like bark, inorganic matter, and petroleum products. Our research highlights the need for improved fuel quality control measures, and transparent and accurate product labeling, as well as the need for a comprehensive and publicly available national database of solid biomass fuel producers and fuels sold. These are essential steps toward increasing customers’ awareness and trust, encouraging them to embrace biomass fuels as reliable and sustainable sources of energy.
Toxicity and bioconcentration of Bisphenol A alternatives in the freshwater pulmonate snail Planorbella pilsbryi
Authors: Gilroy Ève AM , Robichaud Karyn , Villella Maria , Chan Kara , McNabney David WG , Venier Carmen , Pham-Ho Victor , Strub Emilie Montreuil , Ravary Shelby A , Prosser Ryan S , Robinson Stacey A
Published: January 2024

The toxicity of Bisphenol A (BPA) and replacement products Bisphenol F (BPF), Bisphenol S (BPS), and Bisphenol AF (BPAF) was assessed in freshwater snail Planorbella pilsbryi embryos and adults. The chronic toxicity of BPA and BPAF was further characterized in 28-d tests with adult snails, followed by 21-d assessments of hatching and survival of embryos produced at the end of the test (F1 generation). BPAF was the most toxic of the compounds tested, followed by BPA, BPF, and BPS. In the chronic test with BPA, although we observed no significant effects on adult snails, the hatching and survival of juveniles from the F1 generation was affected at the highest concentration (Maximum Acceptable Toxicant Concentration [MATC]: 0.032 mg/L). We did not observe the same differences during exposure to BPAF. Though some overlap existed, effects were observed at concentrations above most reported environmental exposure values. Given that concentrations of alternative products are expected to increase, and in the absence of data on potential effects of mixtures, further research is needed.
Application of an artificial deaminase system for restoration of mutated mRNAs in the macular mouse caused by T>C mutation
Authors: Tsukahara Toshifumi , Bhakta Sonali , Kodama Hiroko , Mimaki Masakazu
Published: January 2024

RNA editing is a significant mechanism underlying genetic variation and protein molecule alteration; C-to-U RNA editing, specifically, is important in regulation of mammalian genetic diversity. The ability to define and limit access of the enzymatic machinery, to avoid modification of unintended targets, is key to the success of RNA editing. Identification of the core component of the apoB RNA editing holoenzyme, APOBEC, and investigation of new candidate genes encoding other elements of the complex could reveal further details of APOBEC mediated mRNA editing. Menkes disease is a recessive X chromosome-linked hereditary syndrome in humans, caused by defective copper metabolism due to mutations in theATP7Agene, which encodes a copper-transport protein. Here, we generated plasmids encoding the MS2 system and the APOBEC1 deaminase domain and used a guide RNA with flanking MS2 sites to restore mutatedAtp7ain fibroblasts from the macular mouse model of Menkes disease having T > C mutation. Around 35% of the mutated C nucleotide was restored to U, demonstrating that our RNA editing system is reliable and has potential for therapeutic clinical application. RNA base editing via human RNA-guided cytidine deaminases is a potentially attractive approach forin vivotherapeutic application and provides opportunities for new developments in this field.
Investigating snow avalanche in the Sikkim Himalaya through coherence and displacement mapping of Sentinel-1A SAR data
Authors: Devishri Kangjam , Thombson Chungkham , Rajkumar Kamaljit Singh , Maisnam Mamata , Pallipad Jayaprasad
Published: January 2024

A tragic incident occurred on April 4, 2023, in the Indian state of Sikkim, a part of the Eastern Himalayas. An avalanche hit the Nathula border area and trapped many tourists beneath the snowcovered debris. Avalanches usually happen after the foundation of the material has been weakened by intense precipitation. Climate change has intensified the frequency and intensity of avalanches in the past few years. Our study investigated the snow avalanche using satellite remote sensing techniques. We have used Sentinel-1A Single Look Complex products to study the coherence, line of sight (LOS) displacement, and vertical displacement of the region using the Differential Synthetic Aperture Radar Interferometry (DInSAR) technique. We found a substantial loss of coherence, increased LOS, and vertical displacement in the study region during the second half of March 2023 and the first half of April 2023. This observation coincides with the large-scale damage that occurred during this period. Estimations of vertical displacement associated with landslides and earthquakes are common. However, such observations in snow avalanche analysis are rare. Our study will significantly contribute to expanding the applications and usefulness of radar interferometry.
Perceptions and experiences of clinicians treating tobacco use among cannabis users in substance use treatment programs: A qualitative study
Authors: Martinez Cristina , Pla Marga , Feliu Ariadna , Enríquez Marta , Saura Judith , Cabezas Carmen , Colom Joan , Suelves Josep M. , Mondon Silvia , Barrio Pablo , Andreu Magalí , Raich Antònia , Bernabeu Jordi , Roca Xavier , Guydish Joseph , Fernández Esteve , study Group of clinicians involved in the recruitment of the
Published: January 2024

Background
The global demand for treatment of cannabis use disorder has significantly increased, prompting a need to understand effective strategies for addressing concurrent cannabis and tobacco use. This study focuses on clinicians' experiences and perceptions in delivering smoking cessation services to cannabis users.
Methods
Three focus groups consisting of substance use professionals in Catalonia, Spain, were analyzed using Atlas-ti software, revealing five main themes and 17 subthemes: (i) User characteristics; (ii) Professional characteristics; (iii) Models of intervention; (iv) Organizational healthcare models; and (v) Health policies. Clinicians stressed the importance of intervention models and the active role of professionals in addressing tobacco use within routine care.
Results
Clinicians believed that tobacco cessation could mitigate social isolation and chronic issues among cannabis users, especially those engaged in polydrug use. Recommendations included integrating smoking cessation into all services, reducing healthcare service fragmentation, improving resource accessibility, enhancing clinical documentation, and advocating for stronger population-level tobacco control policies.
Conclusions
Clinicians suggested adopting a personalized therapeutic approach, implementing a more comprehensive model with increased resources, and reinforcing population-level tobacco control policies to enhance intervention effectiveness.
OTOP1: A New Candidate Gene for Non-syndromic Peg Lateralis
Authors: lee Jae-Hoon , choi junglim , Kim Sungnam , Ahn Hyunsoo , Kim Donghyo , Cho Sung-Won , Kim Sanguk
Published: January 2024

Although peg-shaped lateral incisors are a common dental anomaly, the genetic mechanisms underlying peg-shaped lateral incisors are poorly understood, particularly in cases without associated anomalies. The present study aimed to identify potential candidate genes contributing to the development of non-syndromic peg lateralis, by performing whole-exome sequencing (WES). Saliva samples were collected from 20 cases of unrelated Korean individuals that were; not associated with other anomalies. WES was conducted on these samples, and variants were filtered using criteria of a p-value < 0.05, a false discovery rate < 10− 10, and an odds ratio > 1. In silico mutation impact analysis was performed using Polymorphism Phenotyping v2, sorting intolerant from tolerant, and integrated score of co-evolution and conservation algorithms. We identified a heterozygous OTOP1 gene allele encoding the Otopetrin-1 protein, a proton channel, in all 20 individuals. Gene Ontology analysis revealed an association between OTOP1 and peg lateralis. We further confirmed that the peg lateralis candidate variant, rs199742451, of the same genotype was found in the family member of three subjects with the same phenotype. The results suggest a new possible function of OTOP1, which is yet to be studied, and identified it as a new candidate contributing to the development of peg lateralis. This study provides new insights into the genetic basis of non-syndromic peg lateralis and has important implications for further studies on the role of new genes in peg lateralis
Stepdown Infusion of Barbiturate improves Neurological Function in a New Rat Model of Rebleeding Subarachnoid Hemorrhage.
Authors: Kajiwara Sosho , Hasegawa Yu , Fujimori Kana , Morioka Motohiro
Published: January 2024

The manuscript complies with all instructions to authors. Furthermore, authorship requirements have been met and the manuscript has been approved by all the authors. The manuscript has not been published elsewhere, nor is it under consideration by another journal.
All experiments were approved by the Institutional Animal Care and Use Committee of Kurume University and all applicable institutional guidelines for the care and use of animals were followed.
The authors declare that they have no conflicts of interest. The ARRIVE checklist was used in the current study. This study was funded by JSPS KAKENHI (grant number 23K08530), Taiju Life Social Welfare Foundation, and IUHW Research Grants.
CryoNeFEN: High-resolution reconstruction of cryo-EM structures using neural field network
Authors: Liu Manhua , Huang Yue , Chengguang Zhu
Published: January 2024

The elucidation of three-dimensional (3D) structures is crucial to unraveling protein function and illuminating mechanisms in structural biology. Cryogenic electron microscopy (cryo-EM) single-particle analysis provides direct measurements to determine the structures of macromolecules. However, the main challenge is reconstructing high-resolution 3D structures from extremely noisy and randomly oriented 2D projection images. Most existing methods primarily involve the optimization of multiple 2D slices in the Fourier domain but ignore the anisotropy among these slices, thus limiting the reconstruction of high-frequency structures. In this paper, we propose a cryo-EM neural field reconstruction network (cryoNeFEN), a new 3D spatial domain optimization paradigm, that learns a directional isotropic representation of the cryo-EM structure by mapping spatial coordinates to corresponding density values. We qualitatively and quantitatively evaluate cryoNeFEN on four experimental datasets. The results demonstrate the improved directional isotropy and 3D density resolution beyond the limits of existing algorithms in homogeneous reconstruction and resolve the missing elements of SARS-CoV-2 distinctly in heterogeneous reconstruction.
Functions IRBs on Saudi Arabia: what researchers think of IRB roles using (IRB-RAT) tool
Authors: AlFattani Areej , AlShahrani Asma , AlBedah Norah , Alkawi Ammar , AlMeharish Amani , Altwaijri Yasmin , Omar Abeer , AlKawi M. Zuheir , Khogeer Asim
Published: January 2024

Background: The ethics committee has the responsibility to comply with the rules and guidelines regarding oversight of all human research activities, particularly when the research study involves vulnerable people. It also has the role of educating researchers on ethical issues, scientific truthfulness, preventing misconduct and conflicts of interest. In our study we evaluate and benchmark the function of the local ethical committees across the country from the researchers point-of-view.
Methods: We employed an online IRB-RAT survey to measure perspectives of investigators towards IRB functions dealing with fairness issues, services, bias, and competences and upholding the rights of the human participants. Two responses were recorded: first shows how important an IRB function is for the investigator in his work, second shows how researchers rate their IRBs in being descriptive in that specific function. The difference of these two scores represent the outcome.
Results: We had 179 participants, 166(94%) researchers/research coordinators, and 13(7.2%) IRB members, 94 (53%) participants had been working in the research field for more than 11 years, and the majority 163(90%) revealed that they had IRB contact. The study participants’ have recognized the following IRB characteristics as vital: the absence of reviewer and committee biases, IRB respects the investigators, IRB takes into consideration the protection of human participants, IRB responses in timely manner, and that IRB maintains complete and accurate records.
Conclusion: The measuring tool in this study is applicable to assess the function of IRBs in Saudi Arabia. Further collaborations needed to enhance IRBs performance and to engage researchers into more productive communication with their IRBs.
Cultural adaptation and validation of the Sinhala version of the Spiritual Needs Assessment for Patients (S-SNAP) questionnaire
Authors: Ramadasa Udayangani , Silva Shehan , Perera Suraj , Lekamwasam Sarath
Published: January 2024

Background
Spiritual support for patients and caregivers of critically ill patients is associated with improved quality of life. This aspect, however, is not incorporated into the current care pathways in Sri Lanka. The Spiritual Needs Assessment for Patients (SNAP) questionnaire, comprised of 3 domains: psychosocial, spiritual and religious, gives a platform for clinicians to assess the spiritual needs of those patients. This study presents the results of validation of the Sinhala version of the SNAP (S-SNAP) questionnaire.
Methods
The SNAP was translated from English to Sinhala using the standard forward and backward translation process. After verifying the content validity, unambiguity and clarity of items in a focused group discussion, and a pilot study, the pre-final version was tested among 267 volunteers with cancer selected from three state-run cancer care institutions. Data were analysed for internal consistency and item-total correlations. Factor analysis was done using Varimax rotation with Kaiser normalization. A Scree plot was also made to determine the number of factors.
Results
The mean (SD) age of subjects was 63.2 (11.4) years. The total S-SNAP score ranged from 22–88 (maximum 88). The overall Cronbach’s alpha was 0.94 while item-total correlations varied from 0.26–0.87. Total SNAP score showed inverse correlations with age, Charleson Comorbidity index and Barthel index while a positive correlation was seen with the Karnofsky performance status scale (p < 0.05). Kaiser-Meyer-Olkein value of 0.92 (P = < 0.001) for Bartlett’s test indicated adequate sampling and non-linearity of factors. The scree plot showed a four-factor structure explaining 76% variation. Meaning of life and relationship with a supernatural being and religious rituals are loaded as 2 different factors. Worries, fears and forgiveness are grouped as the third factor while relaxation, coping and sharing feelings are loaded separately.
Conclusions
The S-SNAP is a reliable and valid tool to assess spiritual suffering among patients with cancers conversant in the Sinhala language.
Uterine Preservation With Acar’s Atony Suture for Postpartum Uterine Atony
Authors: Acar Ali , Alan Cemre
Published: January 2024

OBJECTIVE
The leading cause of maternal death is postpartum haemorrhage (PPH) worldwide. Uterine atony is the main reason for postpartum haemorrhage. Uterine compression suture techniques have been described to control PPH. The aim of this study is to describe a new compression suture technique which is easy to apply and effective to stop PPH immediately.
METHODS
This is a retrospective study of all women received Acar’s uterine compressive atony suture between January 2021-November 2023 at a single tertiary hospital.
RESULTS
The new suture technique was successful in all cases.
CONCLUSION
Acar’s atony suture was effective to stop PPH due to uterine atony.
Female sex hormones enhance the negative effects of air pollution on lung cells
Authors: Smelan J. W , Frias D. P. , Oliveira J. N.A , Strauss B , Antunes F. , Teodoro W. R. , Mauad T , Macchione M
Published: January 2024

This study aims to investigate whether diesel exhaust particles (DEP) alone or associated with the hormones 17β-estradiol (E2) and progesterone (P4) can interact and alter cellular responses in the hormones and xenobiotic pathways in human bronchial cells (BEAS-2B) and human lung carcinoma (A549) in the following conditions: control (CT); 10 µg/ml DEP; 1 nM E2; 10 nM P4; 1 nM E2+ 10 µg/ml DEP and 10 nM P4+ 10 µg/ml DEP with 72 hours for hormones and 8 hours for DEP. Were studied the expression and localization of ERβ, PRβ and AhR receptors, using the immunofluorescence technique; the activation of the promoter regions ERE, PRE and XRE was using the luciferase “reporter” gene assay and were measured the following cytokines: GM-CSF, IL-6, IL-10, IL-1B, RANTES and TNF-α to determine the inflammatory response using the multiplex assay. The results showed ERβ, PRβ and AhR translocation from cytoplasm to nucleus in the presence of E2 and E2+DEP, to both cells, but the same did not occur when BEAS-2B were exposed P4 + DEP. The results related with XRE and PRE showed activation of their promoter region when cells were exposed to DEP and showed a promoter inhibition response to DEP + P4. ERE did not show any significant response. The exposition of E2 and E2+DEP increased pro-inflammatory cytokines in BEAS-2B while DEP and E2+DEP increased pro-inflammatory cytokines in A549 and the anti-inflammatory cytokine IL-10 responded to E2 in both cells. BEAS-2B present their cellular responses more intensely when exposed to E2 + DEP and an increase in the expression of ERβ and PR receptors with concomitant increase in inflammatory cytokines GM-CSF, IL-6 and TNF-α. ERE promoter region was not activated, but it does not indicate that DEP does not interfere with the oestrogen pathway, maybe for another way. As conclusion, this study corroborates the hypothesis that air pollution interferes with the pathways of female sex hormones, potentially interfering with the health of the population, especially women.
P123-CoMgAl nanocomposite for sustainable and high-conversion epoxide ring-opening polymerization
Authors: Xu Xiao , Cao Xiaoyan , Yang Yipeng , Chen Fangyi , Gu Zhenggui
Published: January 2024

The ring-opening polymerization lays the foundation for synthesizing polypropylene glycol (PPG). Nevertheless, homogeneous alkali catalysts generally necessitate intricate posttreatments, hampering green synthesis and ensuring environmental sustainability. Here, we develop a heterogeneous epoxide polymerization method based on a P123-modified nanocomposite layered double oxide (CoMgAl-LDO (P123)). Benefiting from the designable and self-assembled P123 micelles, CoMgAl-LDO (P123) yields intergranular gaps and consistent mesoporous channels for effective catalysis. Consequently, CoMgAl-LDO (P123) has a large surface-to-volume ratio of 190.0 m2 g− 1 and a pore volume of 1.0 cm3 g− 1. More importantly, the epoxide catalytic conversion approaches 96.3%, which is higher than the 92% conversion of other solid catalysts. Our strategy addresses the trade-off issue that alkali catalysts face in terms of sustainability and operational complexity, holding great promise in green chemistry.
Expanding the phenotypic spectrum of NOTCH1 variants: Clinical manifestations in families with congenital heart disease
Authors: Jobling Rebekah , Stanley Kaitlin , Kalbfleisch Kelsey , Moran Olivia , Chaturvedi Rajiv , Roifman Maian , Manshaei Roozbeh , Martin Nicole , McDermott Simina , McNiven Vanda , Myles-Reid Diane , Nield Lynne , Reuter Miriam , Schwartz Marci , Shannon Patrick , Silver Rachel , Somerville Cherith , Teitelbaum Ronni , Zahavich Laura , Bassett Anne , Kim Raymond , Mital Seema , Chitayat David
Published: January 2024

Pathogenic variants in NOTCH1 are associated with non-syndromic congenital heart disease (CHD) and Adams-Oliver syndrome (AOS). The clinical presentation of individuals with damaging NOTCH1 variants is characterized by variable expressivity and incomplete penetrance; however, data on systematic phenotypic characterization are limited. We report the genotype and phenotype of a cohort of 33 individuals (20 females, 13 males; median age 23.4 years, range 2.5–68.3 years) from 11 families with causative NOTCH1 variants (9 inherited, 2 de novo; 9 novel), ascertained from a proband with CHD. We describe the cardiac and extracardiac anomalies identified in these 33 individuals, only one of whom met criteria for AOS. The most common CHD identified was tetralogy of Fallot, though various left- and right-sided lesions and septal defects were also present. Extracardiac anomalies identified include cutis aplasia (5/33), cutaneous vascular anomalies (7/33), vascular anomalies of the central nervous system (2/10), Poland anomaly (1/33), pulmonary hypertension (2/33), and structural brain anomalies (3/14). Identification of these findings in a cardiac proband cohort supports NOTCH1-associated CHD and NOTCH1-associated AOS lying on a phenotypic continuum. Our findings support: 1) Broad indications for NOTCH1 molecular testing (any familial CHD, simplex tetralogy of Fallot or hypoplastic left heart); 2) Cascade testing in all at-risk relatives; and 3) A thorough physical exam, in addition to cardiac, brain (structural and vascular), abdominal, and ophthalmologic imaging, in all gene positive individuals. This information is essential for guiding the medical management of these individuals, particularly given the high prevalence of NOTCH1 variants in the CHD population.
Risk factors for subchondral bone alterations in temporomandibulat joint arthralgia: a retrospective multivariate CBCT study
Authors: Emshoff Rüdiger , Rudisch Ansgar , Bertram Stefan
Published: January 2024

Objectives
Temporomandibular joint (TMJ) osteoarthritis (OA) is a progressive degenerative joint disorder characterized by loss of cartilage and alterations in subchondral bone architecture. Prospective studies using multivariable analysis to identify risk factors for subchondral bone alterations (SBAs) are lacking. The aim of this study was to identify clinical baseline predictors mostly associated with the severity of TMJ condylar SBAs.
Materials and methods
This retrospective paired-design study enrolled 233 consecutive TMJ pain patients. TMJ pain patients were classified according to the diagnostic criteria for temporomandibular disorders (DC/TMD) Axis I protocol. Following clinical examination, each patient underwent cone beam computed tomography (CBCT) evaluation of both TMJs. A composite condylar SBA grading score was created from the following items: erosion, resorption, cyst, sclerosis, osteophyte, and flattening; the maximum possible score was 4. Multivariate ordinal regression analysis identified baseline predictors of CBCT condylar SBA grades.
Results
The study group included 87.6% women, and the overall average age of the study population was 41.9 ± 18.1 years. The following factors were significantly associated with the severity of condylar SBAs: older age (> 45 years) (odds ratio [OR], 4.53; p < 0.001), having concomitant arthralgia (OR, 1.85; p = 0.035), and higher number of missing posterior teeth (> 4) (OR, 1.80; p = 0.039). Gender (p = 0.080) and time since pain onset (p < 0.05) had no significant association with condylar SBA grade.
Conclusions
Older age, concomitant arthralgia, and a higher number of missing posterior teeth were related to the severity of TMJ condylar SBAs.
Clinical relevance
Older patients with missing posterior teeth who have a concurrent arthralgia should be advised of their increased risk of developing SBAs. Delayed diagnosis and treatment can be avoided by focusing on these risk factors.
A qualitative interview study to determine barriers and facilitators of implementing automated decision support tools for genomic data access
Authors: Rahimzadeh Vasiliki , Baek Jinyoung , Lawson Jonathan , Dove Edward S.
Published: January 2024

Data access committees (DAC) gatekeep access to secured genomic and related health datasets yet are challenged to keep pace with the rising volume and complexity of data generation. Automated decision support (ADS) systems have been shown to support consistency, compliance, and coordination of genomic data sharing of data access review decisions. However we lack understanding of how DAC members perceive the value add of ADS, if any, on the quality and effectiveness of their reviews. In this qualitative study, we report findings from 13 semi-structured interviews with DAC members from around the world to identify relevant barriers and facilitators to implementing ADS for genomic data access management. Participants generally supported pilot studies that test ADS performance for example in cataloging data types, verifying user credentials and tagging datasets for use terms. Concerns related to over-automation, lack of human oversight, low prioritization, and misalignment with institutional missions tempered enthusiasm for ADS among the DAC members we engaged. Tensions for change in institutional settings within which DACs operated was a powerful motivator for why DAC members considered the implementation of ADS into their access workflows, as well as perceptions of the relative advantage of ADS over the status quo. Future research is needed to build the evidence base around the comparative effectiveness and decisional outcomes of institutions that do/not use ADS into their workflows.
The analysis of bioinformatics uncovers the interaction genes and immune connection between the fracture and CRPS
Authors: Kong Yan , Wang Jian , Guo Qulian , Luo Sumei , Ding Zhuofeng
Published: January 2024

Background
Patients with fractures are known to have an increased susceptibility to the development of complex regional pain syndrome (CRPS), yet the precise underlying mechanism of this condition remains inadequately understood. The objective of this study is to identify shared genes between fracture and CRPS and explore their potential molecular mechanisms.
Methods
The raw data pertaining to facture and CRPS were obtained from the Gene Expression Omnibus (GEO) datasets. Venn plots were utilized to extract the differentially expressed genes (DEGs) that were shared between facture and CRPS. Subsequently, functional enrichment analyses were conducted on these shared genes to identify significant biological functions. The PPI network was employed to visualize the network of the shared genes, and the hub genes were identified using MCODE. The levels of immune infiltration in fracture and CRPS were evaluated using the CIBERSORT algorithm, and the relationship between hub genes and immune cell infiltration was investigated. Additionally, the expression levels of hub genes were examined to explore potential phenotypic subgroups in fractures and CRPS, and the infiltration of immune cells was analyzed across different subcategories.
Results
A comprehensive analysis revealed the presence of 13 overlapping genes among the DEGs in the fracture and CRPS datasets. The outcomes of functional enrichment analysis suggest that these shared genes primarily participate in immune-related pathways. Furthermore, our investigation identified five hub genes that exhibited up-regulation in both CRPS and fracture patients when compared to healthy individuals. The analysis of immune infiltration revealed an increase in various immune cell populations among patients diagnosed with CRPS and fractures. Furthermore, the hub genes exhibited strong associations with multiple infiltrating immune cells. Based on their shared gene expression profiles, patients with fractures and CRPS were classified into two distinct clusters, namely C1 and C2.
Conclusion
Our study reveals a close relationship between fracture and CRPS from crosstalk genes, providing clues to further explore the interaction and therapy for fracture and CRPS.
The Correlation between Corneal Shaping Force and Corneal Biological Parameters in Adolescents
Authors: Yu Jia , Zhou Yuehua , Guo Yujuan
Published: January 2024

Purpose
This study aimed to analyze the influence of corneal parameters on the corneal shaping force.
Methods
This retrospective study enrolled 189 patients. According to the different spherical equivalent(SE), grouping as: A, SE≤-2.0D; B,> -2.0D and≤-3.0D; C, > -3.0D and≤-4.0D; D, > -4.0D and≤-5.5D。Some parameters were measured before OK was worn. The shaping force is the difference between the equivalent spheres before and after wearing OK.
Results
One day post-OK, △SE of A was significantly associated with hexagonal cells(6A); B were associated with Em and coefficient of variation (CV); C was associated with horizontal curvature radius(rf); D were associated with height difference and corneal biomechanical index (TBI); in multivariate analyses, △SE were associated with SE and total corneal thickness. For one week, C was associated with the corneal biomechanical index(TBI), and D was associated with rf and Em (P < 0.05). In the multivariate analyses,△SE was associated with SE,SRI, and total corneal thickness(P < 0.001).
Conclusions
The factors affecting the corneal shaping force under different SE were different, and different analyses should be performed on the individualized corneal parameters of children.
Dense Granular Flow Described by Micropolar Fluid and Its Peridynamic Implementation
Authors: Wan Ji , Qu Wenzhong , Chu Xihua
Published: January 2024

This work presents a nonlocal mesh-free peridynamic model for micropolar fluids that describe fluids enriched with the micro-rotational and length scale effects. The stabilized force state is applied to remedy the zero-energy mode instability in the micropolar viscous term. The present model is validated with the planar Couette flow and Poiseuille flow simulation. Considering the natural inheritance of micro-spinning and microstructures in granular flows, the peridynamic micropolar fluid model is also applied to simulate the dense, dry granular flow with a modified µ(I) rheology flow law. The effects of the coupling number, the micro-inertia, and the characteristic length on the granular µ(I) flow are discussed in a two-dimensional column collapse example. The numerical results of column collapse show that the micropolar coupling number can significantly affect column collapse behavior. A larger coupling number can slow down the translational movement of the granular flow, resulting in a larger angle of repose. The micro-rotational velocity increases by enlarging the coupling number. The micro-inertia and characteristic length have a significant influence on the micro-rotational behavior of the granular flow. Increasing either micro-inertia or characteristic length value decreases the micro-rotational velocity. However, the characteristic length and micro-inertia have an insignificant influence on the translational behavior. Slight differences are observed in the translational velocity distribution or free surface profile.
Design of Zigbee Adaptive Wireless Sensor Network Based on RSSI Predictive Value
Authors: Jin Zhu , Hao Wu
Published: January 2024

WSN (Wireless Sensor Network) is the technology used for sensors and communication devices to monitor data, which is characterized by low power consumption, miniaturization, and low cost. The QoS（Quality of Service） of the WSN is an important index to measure its performance, the moving characteristics of the human body and the working environment all affect the quality of the link, so that the RSSI (Received Signal Strength Indicator) is disturbed and the QoS is declined. To solve the above problems, This paper constructs an RPG-RGMM (Reference Point Group-Random Gauss-Markov Human Mobility Model) model considering the mobile characteristics of the human body, and investigates the RSSI decay pattern of ZigBee nodes moving with the body under the influence of the human body movement and the surrounding environment. The RSSI prediction value is used as an influence factor to update the selection of the relay node in the transmission management, forming an adaptive topology routing strategy. The results of the experiment demonstrate a decrease in the total packet loss rate of the adaptive topology routing strategy based on the RSSI prediction value of 10.61% compared to the traditional routing strategy. From a long-term perspective, this application has a good practical value.