bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay
Authors: Tibola Cristiane Maria , Silva Leandro , Sgubin Fernanda , Omoto Celso
Published: July 2021

The novel ingestion bioassay method was developed for detecting and monitoring resistance of Euschistus heros by encapsulating an artificial liquid diet using Parafilm®. This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of E. heros collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated E. heros populations. In order to monitor the susceptibility of E. heros to insecticides, diagnostic concentrations were defined based on the LC99 of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an E. heros strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively.
Horizontal Honey-Bee Larvae Rearing Plates Can Increase the Deformation Rate of Newly Emerged Adult Honey Bees
Authors: Kim Juyeong , Chon Kyongmi , Kim Bo-Seon , Oh Jin-A , Yoon Chang-Young , Park Hong-Hyun , Choi Yong-Soo
Published: July 2021

Rearing honey bee larvae in vitro is an ideal method to study honey bee larval diseases or the toxicity of pesticides on honey bee larvae under standardized conditions. However, recent studies reported that a horizontal position may cause the deformation of emerged bees. Accordingly, the purpose of this study was to evaluate the emergence and deformation rates of honey bee (Apis mellifera ligustica) larvae reared in horizontal and vertical positions. The study was conducted under the same laboratory conditions with three experimental groups, non-capped or capped horizontal plates and capped vertical plates. However, our results demonstrated that the exhibited adult deformation rates of the horizontal plates were significantly higher (27.8% and 26.1%) than those of the vertical plates (11.9%). In particular, the most common symptoms were deformed wings and an abnormal abdomen in the horizontal plates. Additionally, adults reared on horizontal plates were substantially smaller (10.88 and 10.82 mm) than those on vertical plates (11.55 mm). Considering these conclusions, we suggest that a vertical rearing method is more suitable when considering the deformation rates of the control groups to verify the sublethal effects of pesticides on honey bees.
Behavior of Soft Tissue around Platform-Switched Implants and Non-Platform-Switched Implants: A Comparative Three-Year Clinical Study
Authors: Farronato Davide , Manfredini Mattia , Farronato Marco , Pasini Pietro Mario , Orsina Andrea Alain , Lops Diego
Published: July 2021

To verify the influence of platform-switching (PS) on soft tissue behavior by comparing the soft tissue stability around implants with and without PS, during three years of follow-up. The study included patients treated with fixed dentures supported by implants with an internal connection. The radiographic distance between the first bone-to-implant contact (FBIC) and the implant shoulder was assessed. Additionally, the presence of keratinized facial mucosa and the prosthetic crown height (TH) were monitored for three years from the delivery of the definitive crown. These parameters were measured for two different groups: platform-switched implants in the PS group and non-platform-switched (NPS) implants in the NPS group. Seventy-seven implants were considered in the statistical analysis. After three years, the overall FBIC mean value was 0.31 ± 1.00 mm. However, the mean FBIC was 0.66 ± 0.97 mm for the NPS group and −0.05 ± 0.91 mm for the PS group. Moreover, a mean recession of 0.54 ± 1.39 mm was measured for the NPS group, whereas a mean coronal migration of 0.17 ± 0.95 mm was measured for the PS group. A significant correlation was also found between the presence of PS and ΔTH (p ≤ 0.01) over the three years of follow-up. The absence or presence of platform-switching would appear to affect the tendency of the gingival buccal margin towards recession or creeping. Additionally, implant-abutment platform-switching seems to help prevent peri-implant soft tissue recession over time when compared to implants without PS.
The Importance of Abnormal Platelet Count in Patients with Clostridioides difficile Infection
Authors: Buchrits Shira , Gafter-Gvili Anat , Bishara Jihad , Atamna Alaa , Ayada Gida , Eynath Yair , Avni Tomer
Published: July 2021

Background: Clostridium difficile infection (CDI) causes morbidity and mortality. Platelets have been increasingly recognized as an important component of innate and adaptive immunity. We aimed to assess the incidence of thrombocytopenia and thrombocytosis in CDI and the effect of an abnormal platelet count on clinical outcomes. Methods: This single-center, retrospective cohort study consisted of all adult patients hospitalized in Rabin Medical Center between 1 January 2013 and 31 December 2018 with laboratory confirmed CDI. The primary outcome was 30-day all-cause mortality. Risk factors for 30-day all-cause mortality were identified by univariable and multivariable analyses, using logistic regression. Results: A total of 527 patients with CDI were included. Among them 179 (34%) had an abnormal platelet count: 118 (22%) had thrombocytopenia and 61 (11.5%) had thrombocytosis. Patients with thrombocytosis were similar to control patients other than having a significantly higher white blood cell count at admission. Patients with thrombocytopenia were younger than control patients and were more likely to suffer from malignancies, immunosuppression, and hematological conditions. In a multivariable analysis, both thrombocytosis (OR 1.89, 95% CI 1.01–3.52) and thrombocytopenia (OR 1.70, 95% CI 1.01–2.89) were associated with 30-days mortality, as well as age, hypoalbuminemia, acute kidney injury, and dependency on activities of daily living. A sensitivity analysis restricted for patients without hematological malignancy or receiving chemotherapy revealed increased mortality with thrombocytosis but not with thrombocytopenia. Conclusions: In this retrospective study of hospitalized patients with CDI, we observed an association between thrombocytosis on admission and all-cause mortality, which might represent a marker for disease severity. Patients with CDI and thrombocytopenia also exhibited increased mortality, which might reflect their background conditions and not the severity of the CDI. Future studies should assess thrombocytosis as a severity marker with or without the inclusion of the WBC count.
Organoprotective Effects of Spironolactone on Top of Ramipril Therapy in a Mouse Model for Alport Syndrome
Authors: Rubel Diana , Zhang Yanqin , Sowa Nenja , Girgert Rainer , Gross Oliver
Published: July 2021

Angiotensin-converting enzyme inhibitors (ACEi) delay progression of the inherited renal disease Alport syndrome. However, the effect of ACEis weakens gradually due to an “aldosterone escape”. Here, we investigate if an aldosterone antagonist can counteract loss of ACEi-efficacy. COL4A3-/- mice were treated with ramipril (ACEi), starting at 4.5 weeks of age, and spironolactone was added at 7 weeks of age. Lifespan until renal failure, as well as kidney function parameters, were investigated. Dual therapy decreased proteinuria levels compared to ACEi monotherapy. Matrix accumulation, as well as tubulointerstitial and glomerular scar-tissue formation, were significantly reduced compared to untreated mice and ACEi-monotherapy at 75 and 100 days. Lifespan in dual treated mice was extended compared to untreated mice. However, lifespan was not superior to ACEi monotherapy–despite improved urea-nitrogen levels in the dual therapy group. In conclusion, adding the aldosterone-antagonist spironolactone to ACEi therapy further improved kidney function and reduced proteinuria and fibrosis. However, survival was not improved further, possibly due to premature death from side effects of dual therapy such as hyperkalemia. Thus, dual therapy could offer an effective therapy option for Alport syndrome patients with progressive proteinuria. However, the risks of adverse events require close monitoring.
Comparison of Holmium:YAG and Thulium Fiber Lasers on the Risk of Laser Fiber Fracture
Authors: Uzan Audrey , Chiron Paul , Panthier Frédéric , Haddad Mattieu , Berthe Laurent , Traxer Olivier , Doizi Steeve
Published: July 2021

Objectives: To compare the risk of laser fiber fracture between Ho:YAG laser and Thulium Fiber Laser (TFL) with different laser fiber diameters, laser settings, and fiber bending radii. METHODS: Lengths of 200, 272, and 365 μm single use fibers were used with a 30 W Ho:YAG laser and a 50 W Super Pulsed TFL. Laser fibers of 150 µm length were also tested with the TFL only. Five different increasingly smaller bend radii were tested: 1, 0.9, 0.75, 0.6, and 0.45 cm. A total of 13 different laser settings were tested for the Ho:YAG laser: six fragmentation settings with a short pulse duration, and seven dusting settings with a long pulse duration. A total of 33 different laser settings were tested for the TFL. Three laser settings were common two both lasers: 0.5 J × 12 Hz, 0.8 J × 8 Hz, 2 J × 3 Hz. The laser was activated for 5 min or until fiber fracture. Each measurement was performed ten times. Results: While fiber failures occurred with all fiber diameters with Ho:YAG laser, none were reported with TFL. Identified risk factors of fiber fracture with the Ho:YAG laser were short pulse and high energy for the 365 µm fibers (p = 0.041), but not for the 200 and 272 µm fibers (p = 1 and p = 0.43, respectively). High frequency was not a risk factor of fiber fracture. Fiber diameter also seemed to be a risk factor of fracture. The 200 µm fibers broke more frequently than the 272 and 365 µm ones (p = 0.039). There was a trend for a higher number of fractures with the 365 µm fibers compared to the 272 µm ones, these occurring at a larger bend radius, but this difference was not significant. Conclusion: TFL appears to be a safer laser regarding the risk of fiber fracture than Ho:YAG when used with fibers in a deflected position.
Validation of Drifting Buoy Data for Ocean Wave Observation
Authors: Hisaki Yukiharu
Published: July 2021

Drifting buoys collect wave data in the open ocean far from land and in areas with strong currents. However, the validation of the drifting buoy wave data is limited. Here, we compared the drifting buoy wave data, ERA5 wave data, and moored GPS buoy wave data. Data from 2009 to 2018 near the coast of Japan were used. The agreement of the drifting buoy-observed wave parameters with the moored GPS buoy-observed wave parameters is better than that of ERA5 wave parameters, which is statistically significant. In particular, the accuracy of the ERA5 wave heights tends to be lower where the ocean currents are fast. On the other hand, the agreement between the drifting buoy-observed wave heights and the moored GPS buoy-observed wave heights was good even in the areas with strong currents. It is confirmed that the drifting buoy wave data can be used as reference data for wave modeling study.
Working to Supply the Demand: Recent Advances in the Science of Zoo Animal Welfare
Authors: Cronin Katherine A.
Published: July 2021

If animal welfare scientists were economists, we would be saying that the demand for knowledge is increasing faster than the supply [...]
High Androgen Receptor mRNA Expression Is Associated with Improved Outcome in Patients with High-Risk Non-Muscle-Invasive Bladder Cancer
Authors: Sikic Danijel , Taubert Helge , Wirtz Ralph M. , Breyer Johannes , Eckstein Markus , Weyerer Veronika , Kubon Jennifer , Erben Philipp , Bolenz Christian , Burger Maximilian , Hartmann Arndt , Wullich Bernd , Wach Sven , Keck Bastian
Published: July 2021

The role of the androgen receptor (AR) in non-muscle-invasive bladder cancer (NMIBC) remains controversial. We retrospectively analyzed the mRNA expression of AR using RT-qPCR in 95 patients with high-risk NMIBC treated with a bladder-sparing approach and correlated AR with clinical data and recurrence-free survival (RFS), cancer-specific survival (CSS), and overall survival (OS). The mRNA expression of AR and KRT5, i.e., the basal-like subtype, was strongly correlated (rs = 0.456; p < 0.001). AR (p = 0.053) and KRT5 (p = 0.029) mRNA expression was negatively correlated with tumor grade. Kaplan–Meier analyses indicated significantly prolonged CSS (p = 0.020) and OS (p = 0.015) and a trend towards longer RFS (p = 0.051) in patients with high AR expression. High KRT5 expression was associated with significantly longer RFS (p = 0.033), CSS (p = 0.029) and OS (p = 0.030), while high KRT20 expression was associated with reduced RFS (p = 0.042). In multivariable analysis, none of the molecular markers was an independent prognostic factor. When performing a substratification with regard to molecular markers and clinicopathological parameters, high AR expression showed improved OS in patients with high KRT20 mRNA expression (p = 0.041). Women showed significantly longer OS in cases with high AR expression (p = 0.011). High AR was associated with significantly improved CSS in males (p = 0.044) and patients with instillation therapy (p = 0.040), while OS was improved regardless of instillation therapy. Younger patients with high AR expression had significantly improved RFS (p = 0.021), CSS (p = 0.014) and OS (p = 0.007). RFS was also improved in patients with high AR and low expression of either KRT5 (p = 0.003) or KRT20 (p = 0.014), but not in patients with high expression of KRT5 or KRT20. In conclusion, high AR mRNA expression is correlated with KRT5 mRNA expression and is associated with an improved outcome in high-risk NMIBC.
(E)-5-Benzyl-7-(3,4-dimethoxybenzylidene)-3-(3,4-dimethoxyphenyl)-2-phenyl-3,3a,4,5,6,7-hexahydro-2H-pyrazolo[4,3c] Pyridine
Authors: Mora Enda , Zamri Adel , Teruna Hilwan Y. , Frimayanti Neni , Ikhtiarudin Ihsan , Herfindo Noval , Rindiana Elsa Natia
Published: July 2021

A new pyrazolo-pyridine analogue (title compound) was synthesized in two steps. The first stage was synthesis of monoketone curcumin analogue through Claisen–Schmidt reaction. The second stage was synthesis of the title compound through intermolecular cyclization under reflux condition. The structure of the title compound has been confirmed by spectroscopic analysis including UV, FT-IR, HRMS, 1D NMR (1H-NMR, 13C-NMR, 1D-TOCSY), and 2D NMR (COSY, HSQC, HMBC). Based on the DPPH assay, the compound has moderate antioxidant activity, with an IC50 value of 194.06 ± 7.88 µg/mL (0.337 mM).
Variation of Durability and Strength Parameters of Pumice Based Mixtures
Authors: Lehner Petr , Konečný Petr , Ghosh Pratanu
Published: July 2021

The numerical modelling of chloride penetration into concrete is very sensitive to the correct description of the input data. In the recent era, high-performance concrete (HPC), which combines Portland cement and other supplementary cementitious materials, has been gaining attraction due to their desirable material properties and durability. The presented results show the application of the modified approach for the evaluation of the suitability of the time-dependent model for the variation of the diffusion coefficient. The 26 various binary and ternary-based concrete mixtures blended with volcanic pumice pozzolan (VPP) as a major supplementary cementitious material (SCM) are compared with the reference Ordinary Portland Cement mixture. Other SCMs namely fly ash, slag, silica fume, and metakaolin were also utilized in ternary-based concrete mixtures. In-depth statistical analysis was carried out to show the variability and effects of the amount of the volcanic pumice as an SCM on the diffusion coefficient. The mean value and regression via linear approximation of the time-dependent coefficient of variation of the diffusion coefficients were used as well as the Root of Mean Squared Error approach. The presented results are suitable as the component of the input parameters for the durability-related probabilistic assessment of the reinforced concrete structures exposed to chlorides. In addition, the time-dependent ultimate limit state-related data was presented.
Changes in Human Erythrocyte Exposed to Organophosphate Flame Retardants: Tris(2-chloroethyl) Phosphate and Tris(1-chloro-2-propyl) Phosphate
Authors: Bukowska Bożena
Published: July 2021

Tris(2-chloroethyl) phosphate (TCEP) and tris(1-chloro-2-propyl) phosphate (TCPP) are the main representatives of organophosphate flame retardants (OPFRs). The exposure of humans to OPFRs present in air, water, and food leads to their occurrence in the circulation. Thus far, no report has been published about the influence of these retardants on non-nucleated cells like mature erythrocytes. Therefore, the impact of TCEP and TCPP (in concentrations determined in human blood as well as potentially present in the human body after intoxication) on human erythrocytes was evaluated. In this study, the effect of TCEP and TCPP on the levels of methemoglobin, reduced glutathione (GHS), and reactive oxygen species (ROS), as well as the activity of antioxidative enzymes, was assessed. Moreover, morphological, hemolytic, and apoptotic alterations in red blood cells were examined. Erythrocytes were incubated for 24 h with retardants in concentrations ranging from 0.001 to 1000 μg/mL. This study has revealed that the tested flame retardants only in very high concentrations disturbed redox balance; increased ROS and methemoglobin levels; and induced morphological changes, hemolysis, and eryptosis in the studied cells. The tested compounds have not changed the activity of the antioxidative system in erythrocytes. TCPP exhibited a stronger oxidative, eryptotic, and hemolytic potential than TCEP in human red blood cells. Comparison of these findings with hitherto published data confirms a much lower toxicity of OPFRs in comparison with brominated flame retardants.
Extraction of Al from Coarse Al–Si Alloy by The Selective Liquation Method
Authors: Li Bo , Wang Yaowu , Gao Bingliang
Published: July 2021

A selective liquation process to extract Al from a coarse Al–Si alloy, produced by carbothermal reduction, was investigated on the laboratory scale. The products obtained by selective liquation–vacuum distillation were analyzed by X-ray diffraction, inductively coupled plasma optical emission spectrometry and scanning electron microscopy. During the selective liquation process with the use of zinc as the solvent, the pure aluminum in the coarse Al–Si alloy dissolved in the zinc melt to form an α-solid solution with zinc, and most of the silicon and iron-rich phases and Al–Si–Fe intermetallics precipitated and grew into massive grains that entered into the slag and separated with the Zn–Al alloy melt. However, some fine silicon particles remained in the Zn–Al alloy. Thus, Al–Si alloys conforming to industrial application standards were obtained when the Zn–Al alloys were separated by a distillation process.
The Effect of Sr-CoFe2O4 Nanoparticles with Different Particles Sized as Additives in CIP-Based Magnetorheological Fluid
Authors: Nugroho Kacuk Cikal , Ubaidillah Ubaidillah , Arilasita Retna , Margono Margono , Priyambodo Bambang Hari , Purnama Budi , Mazlan Saiful Amri , Choi Seung-Bok
Published: July 2021

This study investigated the effect of adding strontium (Sr)-doped cobalt ferrite (CoFe2O4) nanoparticles in carbonyl iron particle (CIP)-based magnetorheological fluids (MRFs). Sr-CoFe2O4 nanoparticles were fabricated at different particle sizes using co-precipitation at calcination temperatures of 300 and 400 °C. Field emission scanning electron microscopy (FESEM) was used to evaluate the morphology of the Sr-CoFe2O4 nanoparticles, which were found to be spherical. The average grain sizes were 71–91 nm and 118–157 nm for nanoparticles that had been calcinated at 300 and 400 °C, respectively. As such, higher calcination temperatures were found to produce larger-sized Sr-CoFe2O4 nanoparticles. To investigate the rheological effects that Sr-CoFe2O4 nanoparticles have on CIP-based MRF, three MRF samples were prepared: (1) CIP-based MRF without nanoparticle additives (CIP-based MRF), (2) CIP-based MRF with Sr-CoFe2O4 nanoparticles calcinated at 300 °C (MRF CIP+Sr-CoFe2O4-T300), and (3) CIP-based MRF with Sr-CoFe2O4 nanoparticles calcinated at 400 °C (MRF CIP+Sr-CoFe2O4-T400). The rheological properties of these MRF samples were then observed at room temperature using a rheometer with a parallel plate at a gap of 1 mm. Dispersion stability tests were also performed to determine the sedimentation ratio of the three CIP-based MRF samples.
Comparative Investigation of the Experimental Determination of AA5086 FLCs under Different Necking Criteria
Authors: Kong Xiangrui , Chu Xingrong , Chen Chongqian , Wang Yangang , Liu Peixing , Wang Zhihao
Published: July 2021

The construction of a forming limit diagram (FLD) is a conventional approach to obtain limit strains and to evaluate the formability of sheet metal. Appropriate necking criteria should be applied to determine the forming limit curve (FLC) accurately. In recent years, deep research on the determination of the FLC has been carried out; meanwhile, several necking criteria have been proposed. However, the application of inappropriate necking criteria would cause deviations when determining FLCs. In this study, both Marciniak and Nakajima tests were carried out on the AA5086 aluminum sheet to make a comparative investigation of different necking criteria in the determination of FLCs. In the Marciniak test, four existing necking criteria were chosen to construct FLCs, and analyzed in detail. The well-performed time dependent and position dependent methods were selected for the Nakajima test. Meanwhile, the modified Wang method based on the height change of the adjacent points was proposed. The comparative results showed that the time and position dependent methods were relatively conservative in both experiments, while the modified Wang method could identify the onset of localized necking more accurately.
Crack Propagation in As-Extruded and Heat-Treated Mg-Dy-Nd-Zn-Zr Alloy Explained by the Effect of LPSO Structures and Their Micro- and Nanohardness
Authors: Maier Petra , Clausius Benjamin , Richter Asta , Bittner Benjamin , Hort Norbert , Menze Roman
Published: July 2021

The investigation of the crack propagation in as-extruded and heat-treated Mg-Dy-Nd-Zn-Zr alloy with a focus on the interaction of long-period stacking-ordered (LPSO) structures is the aim of this study. Solution heat treatment on a hot extruded Mg-Dy-Nd-Zn-Zr (RESOLOY®) was done to change the initial fine-grained microstructure, consisting of grain boundary blocky LPSO and lamellar LPSO structures within the matrix, into coarser grains of less lamellar and blocky LPSO phases. C-ring compression tests in Ringer solution were used to cause a fracture. Crack initiation and propagation is influenced by twin boundaries and LPSO lamellae. The blocky LPSO phases also clearly hinder crack growth, by increasing the energy to pass either through the phase or along its interface. The microstructural features were characterized by micro- and nanohardness as well as the amount and location of LPSO phases in dependence on the heat treatment condition. By applying nanoindentation, blocky LPSO phases show a higher hardness than the grains with or without lamellar LPSO phases and their hardness decreases with heat treatment time. On the other hand, the matrix increases in hardness by solid solution strengthening. The microstructure consisting of a good balance of grain size, matrix and blocky LPSO phases and twins shows the highest fracture energy.
On Developing a Hydrophobic Rubberized Cement Paste
Authors: Chen Chi-Yao , Shen Zih-Yao , Lee Maw-Tien
Published: July 2021

It is well known that most cement matrix materials are hydrophilic. For structural materials, hydrophilicity is harmful because the absorption of water will induce serious damage to these materials. In this study, crumb rubber was pretreated by partial oxidation and used as an additive to develop a hydrophobic rubberized cement paste. The pretreated crumb rubber was investigated using Fourier-transform infrared spectrometry (FT-IR) to understand the function groups on its surface. The pyrolysis oil adsorbed on the surface of the crumb rubber was observed by FT-IR and nuclear magnetic resonance (NMR) spectroscopy. A colloid probe with calcium silicate hydrate (C–S–H) at the apex was prepared to measure the intermolecular interaction forces between the crumb rubber and the C-S-H using an atomic force microscope (AFM). Pure cement paste, cement paste with the as-received crumb rubber, and cement paste with pretreated crumb rubber were prepared for comparison. FT-IR, X-ray diffraction (XRD), and scanning electron microscopy (SEM) were used to understand the microstructure of the pastes. The static contact angle was used as the index of the hydrophobicity of the pastes. Experimental results showed that the hardened cement paste containing partially oxidized crumb rubber had excellent hydrophobic properties with an insignificant reduction in the compressive strength.
Synthesis and Characterization of Mechanical Properties and Wire Cut EDM Process Parameters Analysis in AZ61 Magnesium Alloy + B4C + SiC
Authors: Sathish Thanikodi , Mohanavel Vinayagam , Ansari Khalid , Saravanan Rathinasamy , Karthick Alagar , Afzal Asif , Alamri Sagr , Saleel C. Ahamed
Published: July 2021

Wire Cut Electric Discharge Machining (WCEDM) is a novel method for machining different materials with application of electrical energy by the movement of wire electrode. For this work, an AZ61 magnesium alloy with reinforcement of boron carbide and silicon carbide in different percentage levels was used and a plate was formed through stir casting technique. The process parameters of the stir casting process are namely reinforcement %, stirring speed, time of stirring, and process temperature. The specimens were removed from the casted AZ61 magnesium alloy composites through the Wire Cut Electric Discharge Machining (WCEDM) process, the material removal rate and surface roughness vales were carried out creatively. L 16 orthogonal array (OA) was used for this work to find the material removal rate (MRR) and surface roughness. The process parameters of WCEDM are pulse on time (105, 110, 115 and 120 µs), pulse off time (40, 50, 60 and 70 µs), wire feed rate (2, 4, 6 and 8 m/min), and current (3, 6, 9 and 12 Amps). Further, this study aimed to estimate the maximum ultimate tensile strength and micro hardness of the reinforced composites using the Taguchi route.
Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies
Authors: Sharkeev Yurii P. , Komarova Ekaterina G. , Chebodaeva Valentina V. , Sedelnikova Mariya B. , Zakharenko Aleksandr M. , Golokhvast Kirill S. , Litvinova Larisa S. , Khaziakhmatova Olga G. , Malashchenko Vladimir V. , Yurova Kristina A. , Gazatova Natalia D. , Kozlov Ivan G. , Khlusova Marina Y. , Zaitsev Konstantin V. , Khlusov Igor A.
Published: July 2021

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7 were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.
Additively Manufactured Zirconia for Dental Applications
Authors: Nakai Hiroto , Inokoshi Masanao , Nozaki Kosuke , Komatsu Keiji , Kamijo Shingo , Liu Hengyi , Shimizubata Makoto , Minakuchi Shunsuke , Van Meerbeek Bart , Vleugels Jef , Zhang Fei
Published: July 2021

We aimed to assess the crystallography, microstructure and flexural strength of zirconia-based ceramics made by stereolithography (SLA). Two additively manufactured 3 mol% yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP: LithaCon 3Y 230, Lithoz; 3D Mix zirconia, 3DCeram Sinto) and one alumina-toughened zirconia (ATZ: 3D Mix ATZ, 3DCeram Sinto) were compared to subtractively manufactured 3Y-TZP (control: LAVA Plus, 3M Oral Care). Crystallographic analysis was conducted by X-ray diffraction. Top surfaces and cross-sections of the subsurface microstructure were characterized using scanning electron microscopy (SEM). Biaxial flexural strength was statistically compared using Weibull analysis. The additively and subtractively manufactured zirconia grades revealed a similar phase composition. The residual porosity of the SLA 3Y-TZPs and ATZ was comparable to that of subtractively manufactured 3Y-TZP. Weibull analysis revealed that the additively manufactured LithaCon 3Y 230 (Lithoz) had a significantly lower biaxial flexural strength than 3D Mix ATZ (3D Ceram Sinto). The biaxial flexural strength of the subtractively manufactured LAVA Plus (3M Oral Care) was in between those of the additively manufactured 3Y-TZPs, with the additively manufactured ATZ significantly outperforming the subtractively manufactured 3Y-TZP. Additively manufactured 3Y-TZP showed comparable crystallography, microstructure and flexural strength as the subtractively manufactured zirconia, thus potentially being a good option for dental implants.
Influence of the Die Height on the Density of the Briquette Produced from Shredded Logging Residues
Authors: Nurek Tomasz , Gendek Arkadiusz , Dąbrowska Magdalena
Published: July 2021

An alternative to plant biomass of various origins are forest logging residues. They differ significantly from other, previously used plant materials. This difference is due to the heterogeneous composition and relatively large size of individual particles. This research on the compaction of this type of shredded material was aimed at determining the influence of the die height on the density and relaxation of briquettes. This parameter is crucial for the proper construction of compaction devices. The measurements were carried out for the same fractional composition of the shredded logging residues, with variable input parameters of the material and process. It was found that the briquette density and relaxation are influenced by the die height, as well as the material moisture content and process temperature. The highest density at maximum compaction pressure (1.40 g·cm–3) was obtained at a moisture content of 16%, temperature of 80 °C, and the lowest die height (195 mm). In the case of the briquette density after ejection from the die, the best results were obtained at the same temperature and die height but at a moisture content of 9%. The tests confirmed that, regardless of the process temperature and material moisture, the briquette density increases as the die height is reduced. The relaxation coefficient of compacted logging residues ranges from 21.7% to 50.1% and depends mainly on the material moisture content and the temperature of the process. The lowest value of the relaxation coefficient (21.7 ± 1.61) was obtained at 9% moisture content, 60 °C temperature, and 220 mm die height.
Waterlogging Resistance Evaluation Index and Photosynthesis Characteristics Selection: Using Machine Learning Methods to Judge Poplar’s Waterlogging Resistance
Authors: Xie Xuelin , Shen Jingfang
Published: July 2021

Flood disasters are the major natural disaster that affects the growth of agriculture and forestry crops. Due to rapid growth and strong waterlogging resistance characteristics, many studies have explained the waterlogging resistance mechanism of poplar from different perspectives. However, there is no accurate method to define the evaluation index of waterlogging resistance. In addition, there is also a lack of research on predicting the waterlogging resistance of poplars. Based on the changes of poplar biomass and seedling height, the evaluation index of poplar resistance to waterlogging was well determined, and the characteristics of photosynthesis were used to predict the waterlogging resistance of poplars. First, four methods of hierarchical clustering, lasso, stepwise regression and all-subsets regression were used to extract the photosynthesis characteristics. After that, the support vector regression model of poplar resistance to waterlogging was established by using the characteristic parameters of photosynthesis. Finally, the results show that the SVR model based on Stepwise regression and Lasso method has high precision. On the test set, the coefficient of determination (R2) was 0.8581 and 0.8492, the mean square error (MSE) was 0.0104 and 0.0341, and the mean relative error (MRE) was 9.78% and 9.85%, respectively. Therefore, using the characteristic parameters of photosynthesis to predict the waterlogging resistance of poplars is feasible.
Fractional Order Magnetic Resonance Fingerprinting in the Human Cerebral Cortex
Authors: Vegh Viktor , Moinian Shahrzad , Yang Qianqian , Reutens David C.
Published: July 2021

Mathematical models are becoming increasingly important in magnetic resonance imaging (MRI), as they provide a mechanistic approach for making a link between tissue microstructure and signals acquired using the medical imaging instrument. The Bloch equations, which describes spin and relaxation in a magnetic field, are a set of integer order differential equations with a solution exhibiting mono-exponential behaviour in time. Parameters of the model may be estimated using a non-linear solver or by creating a dictionary of model parameters from which MRI signals are simulated and then matched with experiment. We have previously shown the potential efficacy of a magnetic resonance fingerprinting (MRF) approach, i.e., dictionary matching based on the classical Bloch equations for parcellating the human cerebral cortex. However, this classical model is unable to describe in full the mm-scale MRI signal generated based on an heterogenous and complex tissue micro-environment. The time-fractional order Bloch equations have been shown to provide, as a function of time, a good fit of brain MRI signals. The time-fractional model has solutions in the form of Mittag–Leffler functions that generalise conventional exponential relaxation. Such functions have been shown by others to be useful for describing dielectric and viscoelastic relaxation in complex heterogeneous materials. Hence, we replaced the integer order Bloch equations with the previously reported time-fractional counterpart within the MRF framework and performed experiments to parcellate human gray matter, which consists of cortical brain tissue with different cyto-architecture at different spatial locations. Our findings suggest that the time-fractional order parameters, α and β, potentially associate with the effect of interareal architectonic variability, which hypothetically results in more accurate cortical parcellation.
Fuzzy Set Qualitative Comparative Analysis on the Adoption of Environmental Practices: Exploring Technological- and Human-Resource-Based Contributions
Authors: Muñoz-Pascual Lucía , Curado Carla , Galende Jesús
Published: July 2021

Our main objective was to analyze which paths can lead to the adoption of environmental practices (PRAC) in firms, for which we developed three original alternative research models. Model 1 involves five sources for the adoption of environmental practices: human resource costs, organizational learning capability, firm size, manager educational level and manager experience. Model 2 adopts five sources for PRAC: human resource costs, information technology support, firm size, manager educational level and manager experience. Finally, Model 3 adopts six sources for PRAC: human resource costs, organizational learning capability, information technology support, firm size, manager educational level and manager experience. Therefore, Model 1 uses the organizational learning capability for PRAC, Model 2 uses the information technology support for PRAC and Model 3 uses both organizational learning capability and information technology support for PRAC. We used a fuzzy set qualitative comparative analysis on 349 small- and medium-sized Portuguese firms in twelve industrial sectors. The results show that organizational learning capability (OLC) and information technology support (ITS) are important sources for the development of PRAC. In this line, the three research models show that there are different pathways that lead to PRAC. These research models also show pathways that lead to the absence of PRAC. Therefore, the qualitative findings show the relevancy of OLC and ITS to PRAC. In addition, our findings indicate that, by focusing on variables such as OLC, a firm can find more paths that lead to PRAC. Additionally, with the combination of OLC and ITS, it must be taken into account that only developing ITS without OLC is riskier when obtaining PRAC.
Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content
Authors: Andreeva Anna , Budenkova Ekaterina , Babich Olga , Sukhikh Stanislav , Dolganyuk Vyacheslav , Michaud Philippe , Ivanova Svetlana
Published: July 2021

Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅L−1) into the nutrient medium, the growth rate of the microalgae biomass increased, and the accumulation of carbohydrates reached 41.1%, 47.9%, and 31.7% for Arthrospira platensis, Chlorella vulgaris, and Dunaliella salina, respectively. Chlorella vulgaris had the highest total carbohydrate content (a mixture of glucose, fructose, sucrose, and maltose, 16.97%) among the studied microalgae, while for Arthrospira platensis and Dunaliella salina, the accumulation of total carbohydrates was 9.59% and 8.68%, respectively. Thus, the introduction of carbohydrates into the nutrient medium can stimulate their accumulation in the microalgae biomass, an application of biofuel production (biohydrogen).
Docosahexaenoic Acid Is Naturally Concentrated at the sn-2 Position in Triacylglycerols of the Australian Thraustochytrid Aurantiochytrium sp. Strain TC 20
Authors: Lee-Chang Kim Jye , Taylor Matthew C. , Drummond Guy , Mulder Roger J. , Mansour Maged Peter , Brock Mina , Nichols Peter D.
Published: July 2021

The Labyrinthulomycetes or Labyrinthulea are a class of protists that produce a network of filaments that enable the cells to glide along and absorb nutrients. One of the main two Labyrinthulea groups is the thraustochytrids, which are becoming an increasingly recognised and commercially used alternate source of long-chain (LC, ≥C20) omega-3 containing oils. This study demonstrates, to our knowledge for the first time, the regiospecificity of the triacylglycerol (TAG) fraction derived from Australian thraustochytrid Aurantiochytrium sp. strain TC 20 obtained using 13C nuclear magnetic resonance spectroscopy (13C NMR) analysis. The DHA present in the TC 20 TAG fraction was determined to be concentrated in the sn-2 position, with TAG (16:0/22:6/16:0) identified as the main species present. The sn-2 preference is similar to that found in salmon and tuna oil, and differs to seal oil containing largely sn-1,3 LC-PUFA. A higher concentration of sn-2 DHA occurred in the thraustochytrid TC 20 oil compared to that of tuna oil.
The Importance of Diet in Predicting the Remission of Urticaria—Determination of Allergen-Specific IgE
Authors: Ungureanu Monica Iuliana , Sachelarie Liliana , Ciorap Radu , Stana Bogdan Aurelian , Croitoru Irina , Popa Tudor Ovidiu
Published: July 2021

Background and Objectives: Different types of food introduced gradually in the diet will expose children to different food allergens, increasing the chance of developing allergic diseases. The aim of our study was to determine if allergen-specific IgE values can influence, depending on the diet, the prediction of remission of urticaria in children. Materials and Methods: This prospective study was conducted in 132 patients diagnosed over two years with urticaria, admitted to “Sf. Maria” Clinical Pediatric Hospital Iaşi. Total IgE assay was performed by ELISA, and determination of specific serum IgE by the CLA System Quanti Scan method (Innogenetics, Heiden, Germany). Data were gathered and statistical analysis was performed using statistical software SPSS, using descriptive and inferential statistics. Results: The determination of specific IgE to food allergens was performed on a total of 132 cases. The values of specific IgE were positive for one or more food allergens in 84 patients (63.64%). The most common allergens involved were: cow’s milk in 33.3% cases, egg white in 22.6% cases, and hazelnuts in 11.9% cases. The specific IgE values for the different types of food included in our study had a predictive value for disease remission. Conclusions: The determination of specific IgE confirms the presence of a particular food allergen and may have predictive value for the future development of an allergic manifestation.
COVID-19 Community Transmission among Healthcare Workers at a Tertiary Care Cardiac Center
Authors: Barry Mazin , Robert Asirvatham Alwin , Temsah Mohamad-Hani , Abdul Bari Syed , Akhtar Muhammad Yasin , Al Nahdi Faizah , Erlandez Richilda , Al-Tawfiq Jaffar A. , Al Khushail Abdullah , Al Hebaishi Yahya
Published: July 2021

Aim: To determine the frequency, mode of transmission, and outcome of Coronavirus Disease 2019 (COVID-19) among healthcare workers (HCWs) in a tertiary care cardiac center in the Kingdom of Saudi Arabia (KSA). Methods: This is a retrospective study of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected HCWs and was conducted from 2 March to 31 December 2020. Data related to the presence of COVID-19 symptoms, mode of transmission, hospitalization, and mortality were collected from the patients’ medical records. Results: Of the 4462 patients tested for COVID-19 by real-time reverse transcriptase polymerase chain reaction (RT-PCR), 203 (4.5%) HCWs were positive; of these, 125 (61.6%) were males, and the most common age group was <40 years. The most commonly encountered health professionals were nurses (74, 36.4%), followed by therapists/technicians (48, 23.6%), housekeepers (25, 12.3%), and physicians (21, 10.4%). The majority (184, 90.6%) of the HCWs contracted COVID-19 in the community, and only 19 (9.4%) were healthcare-associated infections. Of the infected HCWs, 169 (83.3%) had mild symptoms and were managed in home isolation. The most common symptoms were fever (128, 63.1%), body ache (124, 61.8%), headache (113, 55.7%), dry cough (123, 60.6%), sore throat (97, 47.8%), body weakness (97, 47.8%), and fatigue (94, 46.3%). Comparing males and females, there was a significantly higher number of female nurses; in contrast, there was a higher number of male physicians, housekeepers, therapists/technicians, and other specialty HCWs. A significantly lower number of nurses, therapists/technicians were infected in the ≥40 years age group compared to <40 years. Furthermore, a significantly higher difference was observed among non-Saudi nurses compared to Saudi nurses. No mortality was documented among the included HCWs. Conclusions: In the largest tertiary cardiac center in KSA, most HCWs who contracted COVID-19 developed mild symptoms; nurses and those aged <40 years were most commonly infected, and most infections were acquired in the community. HCWs’ adherence to mitigation measures outside of the workplace is vital to curb the current pandemic and decrease nosocomial transmission risk.
Fatigue Performance of an Additively Manufactured Zr-Based Bulk Metallic Glass and the Effect of Post-Processing
Authors: Sohrabi Navid , Hamidi-Nasab Milad , Rouxel Baptiste , Jhabvala Jamasp , Parrilli Annapaola , Vedani Maurizio , Logé Roland E.
Published: July 2021

Fatigue is the most common cause of failure of mechanical parts in engineering applications. In the current work, we investigate the fatigue life of a bulk metallic (BMG) glass fabricated via additive manufacturing. Specimens fabricated via laser powder-bed fusion (LPBF) are shown to have a fatigue ratio of 0.20 (fatigue limit of 175 MPa) in a three-point bending fatigue test. Three strategies for improving the fatigue behavior were tested, namely (1) relaxation heat treatment, giving a slight fatigue life improvement at high loading conditions (≥250 MPa), (2) laser shock peening, and (3) changing the build orientation, the latter two of which yielded no significant effects. It was found that the presence of lack of fusion (LoF) had the preponderant effect on fatigue resistance of the specimens manufactured. LoF was observed to be a source of stress localization and initiation of cracks. The fatigue life in BMGs fabricated by LPBF is thus primarily influenced by powder quality and process-induced defects, which cannot be removed by the post-treatments carried out in this study. It is believed that a slight increase in laser power, either in the near-surface regions or in the core of the specimens, could improve the fatigue behavior despite the associated (detrimental) increase of crystallized fraction.