bluuebunny/crossref_metadata_2025 huggingface dataset:
Free Float and Volatility Effect on Stock Liquidity in Indonesia Stock Exchange
Authors: Viratama Dimaz Irja , Hasnawati Sri , Hendrawati Ernie
Published: October 2022

Liquidity is one of the important things in investing in the stock market. The Indonesia Stock Exchange as a regulator makes and establishes regulations in the capital market to conduct trading in an orderly, fair and efficient manner. One of the amended provisions in the regulation is regarding the free float or the percentage of the number of shares outstanding in the public with the aim of increasing the liquidity of the listed shares. In addition to free float, the risk in investing also needs to be considered. Behind a high return there is a high risk as well. High risk in the volatility of stock returns. High volatility attracts investors to invest in the stock market. Investors with risk-taking tendencies prefer this high-volatility condition because it allows them to earn higher returns, thereby increasing liquidity through the trading volume of these shares. This study involves a number of control variables that together determine market liquidity, namely Stock Return, Firm Size and Stock Price. The analysis was carried out on property and construction sector companies listed in the 2016-2020 period. The analytical method used is multiple linear regression analysis using the E-Views 10 application. The results of the analysis found that free float and volatility have a positive effect on stock liquidity, either by including or including control variables. These results indicate that information about free float and volatility is a consideration in capital market investment decisions.
Research on the Paths of Digital Economy to Promote the Low-carbon Development of China's Energy Industry
Authors: Jing Zhao , Jing Li
Published: October 2022

At present, China's energy industry is undergoing revolutionary changes, in a critical period of new and old kinetic energy conversion and low-carbon transformation, of which the digital economy plays an important role in energy supply, green consumption and clean production. In the face of the problems of heavy secondary industry structure, coal energy consumption, low energy utilization efficiency, and insufficient supply of key minerals of new energy in China's energy industry in the low-carbon transformation, we should actively use the digital economy to promote a high degree of integration between the digital economy and the energy industry, consolidate the digital infrastructure of the energy industry, improve the independent innovation ability of key technologies in the energy industry, promote the low-carbon and green development of China's energy industry.
Direct and Indirect Effects of Yield Contributing Characters on Seed Yield in Greengram (Vigna radiata (L.) Wilczek)
Authors: Saikumar S. Shanmukha , Lal Gaibriyal M. , Lavanya G. Roopa , Sharma Bimal , Prameela S. , Shakeer S.
Published: October 2022

The goal of the current experiment was to estimate the correlation coefficients and to study the direct and indirect effects of various yield contributing traits on seed yield in forty greengram genotypes. The experiment was carried out during Kharif 2021 in a randomized block design with three replications. Correlation studies among the characters revealed that seed yield per plant has highly positive and significant association with characters like plant height (cm) (0.438**, 0.326**), number of primary branches (0.366**, 0.387**), number of clusters per plant (0.786**, 0.669**), number of pods per plant (0.561**, 0.629**), no. of seeds/pod (0.279*, 0.222*), harvest index (%) (0.680**, 0.621**) and biological yield per plant (0.870**, 0.862**) at both genotypic and phenotypic level as per values, respectively, depicting that these are important yield contributing traits. Path coefficient analysis of various quantitative traits indicated that the days to fifty percent pod sett (0.0625, 0.0152), plant height (0.0202, 0.0224), number of pods per plant (0.0050, 0.0259), no. of seeds/pod (0.0375, 0.0046), harvest index (0.5088, 0.5113) and biological yield per plant (g) (0.7233, 0.7621) had the positive direct effect on seed yield per plant followed by days to 50% flowering (-0.0032, -0.0062) and pod length (-0.0556, -0.0011) exhibited the negative direct effect on seed yield per plant in greengram. Therefore, direct selection for these characteristics would help in isolating high-yielding genotypes from highly segregating population.
Effect of Pruning Methods on Growth, Yield and Quality of Cherry Tomato (Solanum lycopersicum var. cerasiforme) c.v- Pusa Cherry, Roja Red & Nagamoti under Polyhouse Condition of Prayagraj
Authors: Rao Rudravaram Laxman , Singh Devi , Prasad V. M. , Antony Prashant
Published: October 2022

The experiment was conducted in the horticulture Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj (UP) During July 2021- October 2021. The experiment was laid out in RBD with 8 Treatments with 3 replications. The results revealed that T2 – (Pusa cherry + Two stem) flowering (30.3 days), days to first fruit picking (68.3 days) and maximum number of flowers per cluster (17.4), number of fruit set per cluster (14.4), fruit yield per plant (3.7 kg), fruit yield per 100m2 (16.3 q), Vitamin-C (22.6), TSS (7.2) and also maximum Benefit cost ratio (1:4).
THE EFFECT OF SOCIAL MEDIA CONTENT ON CUSTOMER ENGAGEMENT AND ITS IMPACT ON CUSTOMER INTENTION
Authors: Miryam Naomi , Antonio Ferdi
Published: October 2022

The rising number of fitness centers compelled business professionals to implement effective marketing techniques. This study aimed to analyze and test the antecedent of content in social media marketing, namely social interaction (SI), service provider interaction (SPI), self-concept (SC), functional information (FI), and entertaining information (EI) on customer engagement, and its effect on continuous usage intention (CUI) and positive electronic word of mouth (E-WOM), moderated by health literacy. The conceptual framework was empirically tested on customers of the fitness club. A quantitative survey employing the cross-sectional data method was done. The respondents were selected based on specific criteria, and data was collected using a Likert-scaled online questionnaire. The sample consisted of 236 respondents, and data analysis was conducted using Partial Least Square-Structural Equation Modelling (PLS-SEM). The results demonstrated that engagement has an effect on continuous usage intention (CUI) and the propensity to spread positive E-WOM (PEW). In addition, Health Literacy has not demonstrated a substantial moderating influence. In exchange, health literacy is characterized as a predictor of moderation. The research outcome may have managerial consequences for the fitness facility administration discussed in this article.
PENGARUH SERVICE QUALITY, POSITIVE EMOTIONAL EXPERIENCE, CUSTOMER VALUE, DAN BRAND AWARENESS TERHADAP CUSTOMER SATISFACTION DAN E-WOM WISATAWAN DI DANAU 3 WARNA KELIMUTU ENDE NTT
Authors: Poetry Githaromansa , Halim Verina
Published: October 2022

Banyak aspek pemasaran pariwisata telah mengalami perubahan dramatis sejak munculnya internet. Pariwisata telah menjadi salah satu industri yang paling terpengaruh oleh teknologi digital. Opini dan ulasan konsumen di berbagai situs web dan platform digital kini memainkan peran penting dalam pilihan konsumen atas kunjungan wisata. Penelitian ini modifikasi dari model penelitian yang dilakukan oleh Stojanovic, Andreu, dan Perez (2017). Selain itu, penelitian ini ingin membandingkan hasil penelitian terbaru dengan penelitian sebelumnya yang dilakukan oleh Stojanovic, Andreu, dan Perez (2017) dan Serra-Cantallops et al., (2020). Tujuan Penelitian ini memiliki dua alur model penelitian yang pertama adalah pengembangan dari penelitian Serra-Cantallops et al., (2020) dan Stojanovic et al. (2017) untuk memberikan gambaran yang menyebabkan wisatawan memberikan opini dan ulasan tentang suatu destinasi di platform digital dengan didasarkan pada konstruk service quality, positive emotional experience, dan customer satisfaction. Kemudian penelitian ini juga mempelajari pengaruh customer value dan brand awareness dalam menjelaskan e-WOM pada wisatawan di Taman Nasional Kelimutu Ende NTT.
ONLINE BUYING INTENTIONS OF SHOPEE CONSUMERS: THE INFLUENCE OF CELEBRITY ENDORSEMENT, SOCIAL MEDIA MARKETING, AND BRAND IMAGE
Authors: Firmanza Muhammad Harda Dimas , Artanti Yessy
Published: October 2022

The development of increasingly modern technology has impacted human civilization and changed consumer behavior related to online shopping intentions. Online shopping applications change consumer behavior from consensually purchasing products to digitally downloading them. Technology in the economic field is designed to make it easier for potential consumers to shop online, which is considered more effective and efficient during the COVID-19 pandemic. This study aimed to analyze celebrity endorsements, social media marketing, and the brand image of online shopping intentions of Shopee consumers. This study focuses on potential Shopee users aged 18–65 using non-probability sampling. Data were collected using online questionnaires and analyzed using the multiple linear regression method. The study's results showed that celebrity endorsement and social media marketing had an insignificant influence on the online buying intentions of Shopee consumers (p = 0.018 and p = 0.000). Meanwhile, the brand image does not significantly influence the online buying intentions of Shopee consumers (p = 0.085). Companies must develop promotional strategies with up-to-date product and service performance upgrades to increase online purchase intentions.
Factors associated with stress, anxiety and depression in intensive care nursing in the context of the COVID-19 pandemic / Fatores associados ao estresse, ansiedade e depressão na enfermagem intensivista no contexto da pandemia de COVID-19
Authors: Alves da Silva Duarte Anamaria , Assunção Ribeiro Kaiomakx Renato
Published: October 2022

Objetivo: descrever por meio da literatura, os fatores associados ao estresse, ansiedade e depressão em profissionais de enfermagem atuantes nos cuidados a pacientes graves acometidos pela COVID-19. Método: revisão integrativa da literatura realizada nos recursos informacionais PubMed; Cochrane, LILACS, BDENF, e SciELO utilizando os descritores: COVID-19; Unidades de Terapia Intensiva; Saúde Mental; Ansiedade; Enfermagem, com artigos publicados entre 2020 e 2021. Resultados: foram selecionados 10 estudos completos para análise interpretativa. Fatores como o desconhecimento da doença, sobrecarga de trabalho, falta de equipamentos de proteção individual dentre outros, foram identificados como principais fatores associados ao desenvolvimento e elevação de ansiedade estresse e depressão em profissionais de enfermagem atuantes nos cuidados aos pacientes graves infectados pela COVID 19. Conclusão: evidenciou-se que os profissionais de enfermagem experimentaram sofrimento psicológico significativo causado pela pandemia de COVID-19. E que esses sofrimentos, perduram tanto em seu ambiente de trabalho quanto em sua vida pessoal.
Association of Missense Variants in VSX2 With a Peculiar Form of Congenital Stationary Night Blindness Affecting All Bipolar Cells
Authors: Smirnov Vasily M. , Robert Matthieu P. , Condroyer Christel , Navarro Julien , Antonio Aline , Rozet Jean-Michel , Sahel José-Alain , Perrault Isabelle , Audo Isabelle , Zeitz Christina
Published: October 2022

Importance

Congenital stationary night blindness (CSNB) is an inherited stationary retinal disorder that is clinically and genetically heterogeneous. To date, the genetic association between some cases with CSNB and an unusual complex clinical picture is unclear.

Objective

To describe an unreported CSNB phenotype and the associated gene defect in 3 patients from 2 unrelated families.

Design, Setting, and Participants

This retrospective case series was conducted in 2021 and 2022 at a national referral center for rare ocular diseases. Data for 3 patients from a cohort of 140 genetically unsolved CSNB cases were analyzed clinically and genetically.

Exposures

Complete ocular examination including full-field electroretinography and multimodal fundus imaging (spectral-domain optical coherence tomography, color, infrared reflectance, and short-wavelength autofluorescence photographs) were performed. The gene defect was identified by exome sequencing and confirmed by Sanger sequencing and co-segregation analysis in 1 family. Screening was performed for genetically unsolved CSNB cases for VSX2 variants by direct Sanger sequencing.

Main Outcomes and Measures

Ocular and molecular biology findings.

Results

The series included 3 patients whose clinical investigations occurred at ages in the early 30s, younger than 12 years, and in the mid 40s. They had nystagmus, low stable visual acuity, and myopia from birth and experienced night blindness. Two older patients had bilateral lens luxation and underwent lens extraction. Full-field electroretinography revealed an electronegative Schubert-Bornschein appearance, combining characteristics of incomplete and complete CSNB, affecting the function of rod and cone ON- and OFF-bipolar cells. Exome sequencing and co-segregation analysis in a consanguineous family with 2 affected members identified a homozygous variant in VSX2. Subsequently, screening of the CSNB cohort identified another unrelated patient harboring a distinct VSX2 variant.

Conclusions and Relevance

This case series revealed a peculiar pan-bipolar cell retinopathy with lens luxation associated with variants in VSX2. Clinicians should be aware of this association and VSX2 added to CSNB diagnostic gene panels.
Factors associated with distress among female cancer survivors at the workplace: A cross-sectional study
Authors: Jin Juhyun , Lee Eunju , Lee Suin
Published: October 2022

Objectives

This study aimed to investigate the levels of fatigue, social support, spiritual well-being, and distress of female cancer survivors at the workplace, and identify factors associated with distress.

Methods

One hundred and eighty-two working female cancer survivors participated from the outpatient ward in two medical institutions in South Korea and they completed questionnaires assessing their general characteristics, fatigue, social support (colleagues and superiors), and spiritual well-being distress (existential and religious well-being). The data were analyzed using descriptive statistics, T-test, one-way ANOVA, correlation, and multiple linear regression with SPSS /WIN18 version.

Results

Most of the participants were breast and thyroid cancer (78.5%), married (46.2%), working periods below 10 years (62.7%) and the average age was 49.7 years. Distress positively correlated with fatigue and significant predictors of distress were “type of work” and “main source of household income” among general characteristics, fatigue, religious well-being, and existential well-being.

Conclusions

Our findings suggest that integrated program including educational and practical factors to reduce fatigue and increase spiritual well-being (i.e., peace, faith, meaning, et al.) can decrease distress. Whereas, the “ambivalence” of God accompanied by high religious well-being (i.e., punishment, abandon, blame, and so on) can rather increase distress. The development of an integrated management system of distress at work can be applied as a practical factor to improve job satisfaction, organizational performance, and quality of life.
Development and validation of police mental health ability scale
Authors: Liao Chengju , Gu Xingmei , He Jian , Jiao Yonggang , Xia Fan , Feng Zhengzhi
Published: October 2022

Objectives

Police officers are generally under long-term occupational stress. Good mental health ability enables them to better deal with emergencies and enhance their combat effectiveness. We aimed to develop the Police Mental Health Ability Scale (PMHAS) to provide a reference for police selection and ability training.

Methods

Through literature analysis, individual interviews, half-open and half-closed questionnaire surveys, and expert consultations, the components of police mental health ability (PMHA) were theoretically constructed. Then, we enrolled 824 in-service police officers who participated in the training in Chongqing City and Sichuan Province from November 2018 to January 2019 and recovered 767 valid questionnaires (recovery rate, 93.08%).

Results

Exploratory factor analysis generated five factors for PMHAS, including cognitive intelligence, emotional catharsis, swift decisiveness, behavioral drive, and reward pursuit, accounting for 58.904% of the variance. Confirmatory factor analysis demonstrated that the model fit well (χ2/df = 1.117, RMSEA = 0.020, GFI = 0.948, CFI = 0.990, IFI = 0.990, TLI = 0.987). The correlation coefficients of factors (r = −0.023 ~ 0.580) were lower than that of each factor and total score (r = 0.477 ~ 0.819). The Cronbach's α coefficients of PMHAS and its factors were 0.606–0.863, and the test–retest reliabilities were 0.602–0.732.

Conclusion

These results suggest that PMHAS is reliable and valid enough for measuring PMHA, which shows that it is a potentially valuable tool for assessing the mental health ability of police officers.
Exploring Gene Expression Profiles in Primary Central Nervous System Vasculitis
Authors: Salvarani Carlo , Paludo Jonas , Hunder Gene G. , Ansell Stephen M. , Giannini Caterina , Parisi Joseph E. , Huston John , Koster Matthew J. , Warrington Kenneth J. , Croci Stefania , Brown Robert D.
Published: October 2022

Objective

This study was undertaken to explore the gene expression profile of primary central nervous system vasculitis (PCNSV).

Methods

Brain specimens of 4 patients with granulomatous vasculitis (GV), 5 with lymphocytic vasculitis (LV), 4 with amyloid β‐related angiitis (ABRA), and 4 normal controls were studied. RNA‐sequencing was performed using the Illumina Hiseq‐4,000 platform and the Illumina TruSeq Total‐RNA library. Student t test and false discovery rate tests were performed for each of the differentially expressed transcripts. Ingenuity Pathway Analysis was used for the pathway expression analysis. CIBERSORT was used to estimate the abundances of different immune cell subsets in the tissues based on gene expression data.

Results

Transcripts differentially expressed between PCNSV and normal brain indicated that endosomal, mitochondrial, and ribosome dysfunction, alterations in protein synthesis, and noncoding RNAs might be involved in PCNSV. Pathway analysis revealed the activation of dendritic cell maturation and antigen processing as well as neuroinflammation in PCNSV versus normal brain, whereas oxidative phosphorylation was inhibited. CIBERSORT estimation of immune cell subsets suggested that activated NK cells, M1 macrophages, memory B cells, and follicular helper T cells were likely to be more prevalent in PCNSV samples. Naïve CD4 T cells and monocytes were mainly estimated to be present in GV and ABRA. Plasma cell and γδ T‐cell signatures were mainly found in LV and normal brain. GV showed higher levels of genes associated with macrophage activities and T cells. ABRA showed higher levels of long noncoding RNAs and miR‐616. LV showed higher levels of genes encoding immunoglobulins.

Interpretation

RNA sequencing confirmed PCNSV heterogeneity. ANN NEUROL 2023;93:120–130
Analysis of shared research data in Spanish scientific papers about COVID‐19: A first approach
Authors: Cerda‐Cosme Roxana , Méndez Eva
Published: October 2022

During the coronavirus pandemic, changes in the way science is done and shared occurred, which motivates meta‐research to help understand science communication in crises and improve its effectiveness. The objective is to study how many Spanish scientific papers on COVID‐19 published during 2020 share their research data. Qualitative and descriptive study applying nine attributes: (a) availability, (b) accessibility, (c) format, (d) licensing, (e) linkage, (f) funding, (g) editorial policy, (h) content, and (i) statistics. We analyzed 1,340 papers, 1,173 (87.5%) did not have research data. A total of 12.5% share their research data of which 2.1% share their data in repositories, 5% share their data through a simple request, 0.2% do not have permission to share their data, and 5.2% share their data as supplementary material. There is a small percentage that shares their research data; however, it demonstrates the researchers' poor knowledge on how to properly share their research data and their lack of knowledge on what is research data.
Ein Hängedach aus Holz für das Olympische Wassersportzentrum in Saint‐Denis
Authors: Touraine Benjamin , Zimmermann Michael , Gross Cécilia , Mériaud Laure
Published: October 2022

Das neue Olympische Wassersportzentrum in Saint‐Denis wird für die Olympischen Spiele 2024 in Paris erbaut und soll danach als Multifunktionsarena weitergenutzt werden. Die nach unten gewölbte Hängedachkonstruktion reduziert nicht nur das Luftvolumen in der Haupthalle und damit den Energieverbrauch der Arena, sondern minimiert als Holzkonstruktion auch den CO2‐Fußabdruck des Gebäudes. Die Dachkonstruktion besteht aus bis zu 89 m langen Holzträgern. Nach seiner Fertigstellung gehört das Wassersportzentrum weltweit zu den größten in Holzbauweise ausgeführten Sportstätten und stellt gleichzeitig einen neuen Weltrekord für weitgespannte Holzkonstruktionen auf. Der folgende Beitrag stellt den Gesamtentwurf und die wichtigsten technischen Merkmale sowie Berechnungsmethoden der Konstruktion vor. Auch das Lang‐ und Kurzzeitverhalten der eingesetzten Materialien (Beton, Stahl, Holz) sowie die nichtlineare Analyse der großen Dachverformungen werden erläutert. Außerdem befasst sich der Beitrag mit der Sensitivität des Dachtragwerks in Hinblick auf Fundamente, Betonsockel und Steifigkeit der Holzverbindungen, die speziell für hohe Torsionslasten und die verschiedenen geometrischen Konfigurationen ausgelegt sind. Darüber hinaus geht der Bericht auf das Montageverfahren und die Steuerung von Fertigungs‐/Montagetoleranzen sowie die Geometrieprüfung vor Ort ein, die für eine solche zugbeanspruchte Struktur unerlässlich sind.
Cribriform pattern in prostate tissues: Predictor for intraductal carcinoma of the prostate based on biopsy and radical prostatectomy pathology
Authors: Shiomi Ei , Kato Renpei , Kanehira Mitugu , Takata Ryo , Sugimura Jun , Nakamura Yasuyuki , Ujiie Takashi , Abe Takaya , Obara Wataru
Published: October 2022

Objectives

This study aims to investigate whether a cribriform pattern on prostate biopsy may be a factor in suspicion of intraductal carcinoma of the prostate after radical prostatectomy.

Methods

This retrospective study assessed 100 men who underwent prostatectomy from 2015 to 2019. Participants were grouped as 76 patients with Gleason pattern 4 and 24 patients without this pattern. All 100 participants underwent retrograde radical prostatectomy and limited lymph node dissection. The same pathologist evaluated all specimens. The cribriform pattern was evaluated with haematoxylin and eosin counterstaining, and intraductal carcinoma of the prostate was evaluated with immunohistochemical analysis of cytokeratin 34βE12.

Results

Patients with intraductal carcinoma of the prostate on immunohistochemical analysis showed a significant tendency to relapse in the postoperative period, and those with the cribriform pattern on biopsy had a significant recurrence rate. In univariate and multivariate analyses, intraductal carcinoma of the prostate confirmed in biopsy tissue was an independent predictor of biochemical recurrence after prostatectomy. The rate of intraductal carcinoma of the prostate confirmation was 28% of cases with a cribriform pattern in biopsy tissue, which was increased to 62% in prostatectomy tissues.

Conclusion

The cribriform pattern in the biopsy tissue may be a predictor for intraductal carcinoma of the prostate.
A strategy to explore the quality markers of Ziziphi Spinosae semen by combining metabolic in vivo study with network pharmacology
Authors: Yan Yan , Liu Jiaxing , Zhang Min , Zhang Yinjie , Shi Biyun , Qin Xuemei , Du Chenhui
Published: October 2022

Ziziphi Spinosae semen (ZSS), the dried and ripe seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, has been used as a sedative in China and other Asian countries for over a millennium. However, its quality markers (Q‐markers) are not completely clear. In this study, Q‐markers selected by a metabolic in vivo study combined with network pharmacology are proposed for ZSS quality control. An UHPLC (ultra‐high‐performance liquid chromatography)‐Q‐Orbitrap‐MS method was developed to identify or tentatively assign 48 components including 21 flavonoid C‐glycosides, 2 flavonoid O‐glycosides, 11 dammarane triterpenoid saponins, 13 alkaloids, and 1 other, using a diagnostic product ion filtering strategy in ZSS. Subsequently, 147 metabolites detected from serum, urine, bile, and feces samples of para‐chlorophenylalanine–induced insomnia rats treated with ZSS aqueous extracts could be linked to their respective parent compounds, including 27 prototypes. Meanwhile, three metabolic networks of flavonoids, saponins, and alkaloids are preliminarily established and potential metabolic pathways are investigated under the insomnia condition. Finally, 12 key bioactive components against insomnia including magnoflorine, caaverine, coclaurine, norisocorydine, genkwanin, juzinrine, apigenin, jujubogenin, kaempferol‐3‐O‐rutinoside, jujuboside A, jujuboside B, and spinosin with the highest degree values in component–target–pathways network were selected as Q‐markers for the quality control of ZSS.
Development of a UPLC–MS/MS method for the determination of active ingredients of Shuang Hu in rat blood and its application in pharmacokinetics
Authors: Jiang Xin Rui , Lei Meng Ying , Qin Liu Xin , Zhou Yan Lin , Wang Yang , Li Xue Jian , Wang Gang
Published: October 2022

A simple and sensitive method using in vivo microdialysis coupled with UPLC–MS/MS was established to evaluate the pharmacokinetics of Shuang Hu tincture (SHZTN). Xevo TQ‐S was used to analyze the active ingredients of mesaconitine, hypaconitine, 4‐hydroxycinnamic acid, ferulic acid and N‐(2, 3‐dimethyl phenyl)‐2‐ aminobenzoic acid of SHZTN. Samples were prepared using a methanol precipitation method and the internal standards lannaconitine and p‐hydroxybenzoic acid were added. The method validation was conducted according to the guidelines of the Pharmacopoeia of China. A good linear range was obtained in the range of 1–2,000 ng/ml. The intra‐day and inter‐day precisions were less than 14.7%, and the accuracy range of all the analytes was −10.5–9.3%. The recovery of each analyte was over 95.5%, and matrix effects can be neglected. After a single dose of 20 mg/kg SHZTN, the area under the curve and peak concentration of the five active ingredients were significantly increased by transdermal compared with oral administration, which indicated the high bioavailability of SHZTN. The time to peak concentration of all compounds was <3.4 h, and the half‐life was <15.4 h, which indicated that the five compounds have the best absorption and rapid elimination. The method was successfully developed and applied to the pharmacokinetic study of SHZTN.
Statin use in patients with hormone receptor‐positive metastatic breast cancer treated with everolimus and exemestane
Authors: Lee Kyoungmin , Noh Eunjin , Moon Seok Joo , Joo Yoonjung Yoonie , Kang Eun Joo , Seo Jae Hong , Park In Hae
Published: October 2022

Background

We analyzed the effect of statins in patients with hormone receptor‐positive (HR+) metastatic breast cancer treated with everolimus + exemestane (EverX).

Materials and Methods

We conducted a nationwide retrospective cohort study using the National Health Insurance database with patients who received EverX for metastatic breast cancer between 2011 and 2019.

Results

Of 224,948 patients diagnosed with breast cancer, 1749 patients who received EverX for at least 30 days were included. Among them, 500 (28.6%) patients were found to take statins with EverX treatment (statin group), and the median duration of this combination was 5.36 months. The median time to treatment duration (TTD) for EverX and the overall survival (OS) were significantly higher in the statin group than in the no‐statin group [7.69 vs. 5.06 months, p < 0.001; 45.7 vs. 26.0 months, p < 0.001, respectively]. Multivariable Cox analysis revealed that the use of statins was associated with prolonged TTD [HR = 0.67 (95% CI, 0.59–0.77)] and OS [HR = 0.57 (95% CI, 0.46–0.70)] for EverX even after adjustment for other covariates.

Conclusion

Statins may have synergistic effects with endocrine therapy with the mTOR inhibitor everolimus, and improve survival in patients with HR+ metastatic breast cancer.
Bartonella henselae vertebral osteomyelitis in a pediatric patient: A case report
Authors: Abu‐Rumeileh Sarah , Beltrami Giovanni , Di Maurizio Marco , Indolfi Giuseppe , Trapani Sandra
Published: October 2022

Bartonella henselae is an unusual and rarely suspected osteomyelitis etiologic agent. We present a case of low back‐pain in a 10‐year‐old female which lead to a challenging diagnostic work‐up due to subtle imaging findings. The diagnosis was Bartonella henselae vertebral osteomyelitis mimicking bone tumor.
Sumatriptan poisoning and its clinical presentation in humans: A case report
Authors: Erfan Talab Evini Peyman , Ohadi Laya , Amiri Farbod , Shadnia Shahin , Mostafazadeh Babak , Forouzesh Mehdi , Hedayatshodeh Mohammad Javad , Rahimi Mitra
Published: October 2022

This case report displays some of the possible complications of sumatriptan poisoning, including nephritic syndrome.
Catalysis – Far from Being a Mature Technology
Authors: Beller Matthias , Bender Michael , Bornscheuer Uwe T. , Schunk Stephan
Published: October 2022

No abstract.
Evaluation of toxicities for intravesical drugs in phase 1 bladder cancer trials
Authors: Doersch Karen M. , Tabayoyong William B. , Bandari Jathin
Published: October 2022

Background

Improving clinical trial design is important for optimizing approval of safe and effective drugs. Phase 1 clinical trials seek to determine phase 2 doses by investigating predefined dose‐limiting toxicities. Traditional definitions of dose‐limiting toxicity may not be applicable to intravesical therapies for bladder cancer. This study compared the frequency of dose‐limiting toxicities and serious adverse events in bladder cancer trials for intravesical therapies to other routes of administration.

Methods

Studies were abstracted from ClinicalTrials.gov and reconciled with a PubMed search. Primary and secondary end points were predefined before data abstraction, and the primary end point was subject–level dose‐limiting toxicity rate. Fisher exact tests were performed with p < .05 designated as significant.

Results

Eighteen intravesical studies and 24 studies with other routes of administration (the per os/intravenous/intramuscular [PO/IV/IM] group) were identified. Dose‐limiting toxicities were reported in 38.9% of intravesical studies, affecting 3.29% of subjects, compared with 30.0% of PO/IV/IM studies representing 4.19% of subjects (p = .52 for study‐level and p = .60 for subject‐level comparisons). Serious adverse events occurred in 53.9% of intravesical studies in 10.3% of subjects versus 91.0% of studies reporting serious adverse events affecting 41.4% of subjects in the PO/IV/IM group (p = .03 for subject‐level and p < .0001 for study‐level comparisons).

Conclusions

There was no difference in subject–level dose‐limiting toxicity rate between intravesical and PO/IV/IM bladder cancer trials. The serious adverse event rate was lower in the intravesical group. Heterogeneity of dose‐limiting toxicity definition may affect interpretation of toxicity in phase 1 bladder cancer clinical trials studying different routes of administration.

Lay summary

Bladder cancer is a common cancer type that may be treated with therapies that are instilled into the bladder and act locally, called intravesical therapies.

This study used publicly available regulatory data from early phase clinical trials to determine whether measures of tolerability used in clinical trials are applicable to intravesical therapies for bladder cancer.
Molecular diagnostics of infectious disease: Detection and characterization of microbial agents in cytology samples
Authors: Caruso Carla R. , Yang Zhongbo
Published: October 2022

Background

Cytology samples are widely used to diagnose various infectious diseases by detection and identification of causative infectious agents, including bacteria, fungi, and viruses. The role of cytopathology in infectious disease has expanded tremendously in the past decades with the advances in molecular techniques. Molecular diagnostic methods, compared to conventional methods, have shown improved patient outcome, reduction in cost, and shortened hospital stay times. The aim of this article is to review molecular testing in cytology samples for diagnosis of infectious diseases.

Methods

The literature search for molecular testing in common cytology samples for diagnosis of infectious diseases was performed. The findings of the studies were summarized. The common cytology samples included in this article were gynecologic specimens, cerebrospinal fluid, bronchoalveolar lavage, and urine samples.

Conclusions

There are a number of molecular diagnostic tests that are available to be used in common cytology samples to detect infectious agents. Each test has its own advantages and limitations. It is our hope that upon reading this review article, the readers will have better understanding of molecular diagnostic testing of infectious diseases utilizing commonly sampled cytology specimens in daily practice.
Internalization of muscularity and thinness ideals: Associations with body dissatisfaction, eating disorder symptoms, and muscle dysmorphic symptoms in at risk sexual minority men
Authors: Convertino Alexandra D. , Elbe Connor I. , Mendoza Rebecca R. , Calzo Jerel P. , Brown Tiffany A. , Siegel Jaclyn A. , Jun Hee‐Jin , Corliss Heather L. , Blashill Aaron J.
Published: October 2022

Objective

In the tripartite influence model, appearance‐ideal internalization is identified as a prominent risk factor for the development of body dissatisfaction and subsequent eating disorder (ED) behaviors. For men, prior research has emphasized the importance of both thin‐ideal internalization and muscular‐ideal internalization in explaining later ED behaviors and muscle dysmorphia (MD) symptoms. Previous research in heterosexual men has shown that the associations between muscular‐ideal internalization and ED or MD symptoms may depend on whether the individual has also internalized the thin ideal. However, this interaction has not been examined in research with sexual minority men (SMM).

Method

The current study collected self‐report data from 452 at risk SMM (i.e., endorsed body dissatisfaction), with ages ranging from 18 to 35 years. Linear regression models were conducted to test the interaction effects between thinness and muscularity internalization on ED symptoms, MD behaviors, and general body dissatisfaction. Simple slopes and the Johnson–Neyman technique were used to investigate significant interaction terms.

Results

Thin‐ and muscular‐ideal internalization were positively associated with muscular appearance intolerance and dietary restriction with no significant interaction. Muscular drive for size was highest when both muscularity internalization and thinness internalization were high. Muscular‐ideal internalization was positively associated with both cognitive restraint and general body dissatisfaction, but only at lower levels of thinness internalization.

Discussion

Given the interacting association between thinness and muscularity internalization and aspects of body dissatisfaction, attitudes, and behavior, prevention and intervention programs for EDs and MDs in SMM should seek to dismantle both thinness and muscularity internalization.

Public Significance Statement

Internalizing—or adopting as one's own—the ideal of a body with low body fat and high muscularity has been shown to lead to muscle dysmorphia and eating disorder symptoms in men. The current study examines whether the combination of thin‐ideal and muscular‐ideal internalization is associated with worse symptoms than either facet alone in sexual minority men. Treatment efforts in sexual minority men should address both types of internalization.
How does the increase in eating difficulties according to the Development and Well‐Being Assessment screening items relate to the population prevalence of eating disorders? An analysis of the 2017 Mental Health in Children and Young People survey
Authors: O'Logbon Jessica , Newlove‐Delgado Tamsin , McManus Sally , Mathews Frances , Hill Suzanne , Sadler Katharine , Ford Tamsin
Published: October 2022

Objective

We examine the test accuracy of the Development and Well‐Being Assessment (DAWBA) eating disorder screening items to explore whether the increased eating difficulties detected in the English National Mental Health of Children and Young People (MHCYP) Surveys 2021 reflect an increased population prevalence.

Methods

Study 1 calculated sensitivity, specificity, and positive and negative predictive values from responses to the DAWBA screening items from 4057 11–19‐year‐olds and their parents, in the 2017 MHCYP survey. Study 2 applied the positive predictive value to data from 1844 11–19‐year‐olds responding to the 2021 follow‐up to estimate the prevalence of eating disorders in England compared to 2017 prevalence.

Results

Parental report most accurately predicted an eating disorder (93.6%, 95% confidence interval: 92.7–94.5). Sensitivity increased when parent and child answers were combined, and with a higher threshold (of two) for children. The prevalence of eating disorders in 2021 was 1% in 17–19‐year‐olds, and .6% in 11–16‐year‐olds—similar to the prevalence reported in 2017 (.8% and .6%, respectively). However, estimates for boys (.2%–.4%) and young men (.0%–.4%) increased.

Discussion

We found tentative evidence of increased population prevalence of eating disorders, particularly among young men. Despite this, the DAWBA screening items are useful for ruling out eating disorders, particularly when parents or carers screen negative, but are relatively poor at predicting who will have a disorder. Data from both parents and children and applying a higher cut point improves accuracy but at the expense of more missed cases.

Public Significance Statement

The prevalence of eating disorders did not markedly change from 2017 to 2021, but we found tentative evidence of an increase, particularly among young men. This is despite larger increases in problematic eating, which need further investigation. The DAWBA screen is best suited to ruling out eating disorders which limits its clinical applications as it would provide many false positives requiring further assessment.
Defining the Biologically Plausible Taxonomic Domain of Applicability of an Adverse Outcome Pathway: A Case Study Linking Nicotinic Acetylcholine Receptor Activation to Colony Death
Authors: Jensen Marissa A. , Blatz Donovan J. , LaLone Carlie A.
Published: October 2022

For the majority of developed adverse outcome pathways (AOPs), the taxonomic domain of applicability (tDOA) is typically narrowly defined with a single or a handful of species. Defining the tDOA of an AOP is critical for use in regulatory decision-making, particularly when considering protection of untested species. Structural and functional conservation are two elements that can be considered when defining the tDOA. Publicly accessible bioinformatics approaches, such as the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool, take advantage of existing and growing databases of protein sequence and structural information to provide lines of evidence toward structural conservation of key events (KEs) and KE relationships (KERs) of an AOP. It is anticipated that SeqAPASS results could readily be combined with data derived from empirical toxicity studies to provide evidence of both structural and functional conservation, to define the tDOA for KEs, KERs, and AOPs. Such data could be incorporated in the AOP-Wiki as lines of evidence toward biological plausibility for the tDOA. We present a case study describing the process of using bioinformatics to define the tDOA of an AOP using an AOP linking the activation of the nicotinic acetylcholine receptor to colony death/failure in Apis mellifera. Although the AOP was developed to gain a particular biological understanding relative to A. mellifera health, applicability to other Apis bees, as well as non-Apis bees, has yet to be defined. The present study demonstrates how bioinformatics can be utilized to rapidly take advantage of existing protein sequence and structural knowledge to enhance and inform the tDOA of KEs, KERs, and AOPs, focusing on providing evidence of structural conservation across species. Environ Toxicol Chem 2023;42:71–87. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Both noise‐floor and tissue compartment difference in diffusivity contribute to FA dependence on b‐value in diffusion MRI
Authors: Yao Junye , Tendler Benjamin C. , Zhou Zihan , Lei Hao , Zhang Lei , Bao Aimin , Zhong Jianhui , Miller Karla L. , He Hongjian
Published: October 2022

Noninvasive diffusion magnetic resonance imaging (dMRI) has been widely employed in both clinical and research settings to investigate brain tissue microstructure. Despite the evidence that dMRI‐derived fractional anisotropy (FA) correlates with white matter properties, the metric is not specific. Recent studies have reported that FA is dependent on the b‐value, and its origin has primarily been attributed to either the influence of microstructure or the noise‐floor effect. A systematic investigation into the inter‐relationship of these two effects is however still lacking. This study aims to quantify contributions of the reported differences in intra‐ and extra‐neurite diffusivity to the observed changes in FA, in addition to the noise in measurements. We used in‐vivo and post‐mortem human brain imaging, as well as numerical simulations and histological validation, for this purpose. Our investigations reveal that the percentage difference of FA between b‐values (pdFA) has significant positive associations with neurite density index (NDI), which is derived from in‐vivo neurite orientation dispersion and density imaging (NODDI), or Bielschowsky's silver impregnation (BIEL) staining sections of fixed post‐mortem human brain samples. Furthermore, such an association is found to be varied with Signal‐to‐Noise Ratio (SNR) level, indicating a nonlinear interaction effect between tissue microstructure and noise. Finally, a multicompartment model simulation revealed that these findings can be driven by differing diffusivities of intra‐ and extra‐neurite compartments in tissue, with the noise‐floor further amplifying the effect. In conclusion, both the differences in intra‐ and extra‐neurite diffusivity and noise‐floor effects significantly contribute to the FA difference associated with the b‐value.
A numerical study of air entrapment during the droplet impact on a solid substrate
Authors: Umesh  , Singh N. K.
Published: October 2022

A numerical investigation is conducted to study the air entrapment phenomenon when two different liquids such as water and diesel droplet are impacted on the solid surface. The beginning of the air entrapment process was observed during droplet impact on a solid substrate forming a dimple underneath the droplet. The air film thus trapped underneath the droplet started evolving into the air bubble. This journey of evolution mainly comprises phases like an inertial retraction of air film, contraction, and pinch‐off of the secondary droplet inside the air bubble for a water droplet impact case. The volume of fluid approach has been utilized to track the progress of air film evolution. The influence of surface wettability has been observed on the evolution of air film into the air bubble by taking four different values of contact angle pertaining to the hydrophilic surface (θ = 10° and 35°) and hydrophobic surface (θ = 90° and θ = 120°). The air bubble was found to get detached from the substrate for the hydrophilic surface (θ = 35°) and observed to remain attached to the substrate for the hydrophobic surface. The variation of pressure underneath the droplet was also investigated as the droplet reaches the substrate. The effect of surface tension has been studied on the evolution of air film by impacting the diesel droplet on the same substrate keeping the same wettability condition (θ = 35°). The lower surface tension of the diesel droplet as compared to the water droplet delayed the process of air film evolution and consequently decreases the retraction speed of air film. Also, the air bubble remains attached to the surface. Furthermore, the air bubble detaches from the surface for an even higher wettability condition (θ = 10°). Thus surface wettability and surface tension become two important factors governing the development of entrapped air film and bubble elimination in many practical applications.
In vivo and in vitro binding of [125I]I‐R‐(+)‐TISCH: A dopamine D1 receptor ligand for studying pancreatic β‐cell mass
Authors: Zhang Yan , Li Guangwen , Sun Yuli , Hong Haiyan , Li Linlin , Luo Yang , Wang Ran , Zhu Lin , Kung Hank F. , Zhu Jinxia
Published: October 2022

Diabetes mellitus (DM) and insulinoma are mainly affected by the status of pancreatic β‐cell mass (BCM). Development of imaging agents for BCM allows to study pancreatic β cells and the relationship between β cells and DM or insulinoma. In this study, we investigated the density of dopamine D1 receptor on the β cells and measured BCM by statistical image processing. The pancreatic uptakes of [125I]I‐R‐(+)‐7‐chloro‐8‐hydroxy‐1‐(3′‐iodopheny1)‐3‐methyl‐2,3,4,5‐tetrahydro‐1H‐3‐benzazepine ([125I]I‐R‐(+)‐TISCH), dopamine D1 receptor tracer, in normal and diabetic rats displayed significant differences at 30 min (1.11 ± 0.08% ID/g vs. 0.63 ± 0.09% ID/g, p < 0.0001). In the presence of SCH23390, the pancreatic uptake of [125I]I‐R‐(+)‐TISCH at 30 min in normal rats was lower (1.01 ± 0.04% ID/g, p < 0.05). Although the blocking was not complete, [125I]I‐R‐(+)‐TISCH showed specific binding signals to the pancreas. Furthermore, the uptakes of [125I]I‐R‐(+)‐TISCH in INS‐1 cells were reduced in the presence of SCH23390 at different concentrations. [125I]I‐R‐(+)‐TISCH displayed a respectable uptake in insulinoma. Overall, [125I]I‐R‐(+)‐TISCH provided specific binding signals to pancreatic β cells. Although the specific signal may not be sufficient for imaging in vivo, the dopamine D1 receptor can still be considered as a potential target for studying BCM. Further investigation will be required to optimize the ligand.