bluuebunny/crossref_metadata_2025 huggingface dataset:
PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription
Authors: Love Luca , Jütte Bianca B , Lindqvist Birgitta , Rohdjess Hannah , Kieri Oscar , Nowak Piotr , Svensson J Peter
Published: March 2024

HIV-1 infection establishes a reservoir of long-lived cells with integrated proviral DNA that can persist despite antiretroviral therapy (ART). Some of these reservoir cells can at anytime be reactivated and reinitiate infection. The mechanisms governing proviral latency the transcriptional regulation of the provirus are complex and have not yet been sufficiently described. Here, we identified a role for histone H3 citrullination, a post-translational modification catalyzed by protein-arginine deiminase type-4 (PADI4), in HIV-1 transcription and latency. We found that PADI4 inhibition by GSK484 reduced HIV-1 transcription after T cell activation inex vivocultures of CD4 T cells from people living with HIV-1 (PLWH). The effect was more pronounced in individuals with active viremia compared to individuals with effective ART. Using cell models of HIV-1 latency, we showed that PADI4-mediated citrullination of histone H3 occurred at the HIV-1 promoter upon T cell stimulation which facilitated proviral transcription. HIV-1 preferentially integrated into genomic regions marked by H3 citrullination and these integrated proviruses were less prone to latency compared to those in non-citrullinated chromatin. Inhibiting PADI4 led to compaction of the HIV-1 promoter chromatin and an increase of HP1α-covered heterochromatin, in a mechanism partly dependent on the HUSH complex. Our data reveal a novel mechanism to explain HIV-1 latency and transcriptional regulation.

Highlights

The PADI4 enzyme stimulates HIV-1 transcription during T cell activation.

PADI4 citrullinates histone H3 at the HIV-1 promoter upon T cell activation and inhibiting PADI4 reduces HIV-1 reactivation inex vivoCD4 T cells from people living with HIV-1.

H3cit is mostly found at gene promoters, and productive HIV-1 proviruses are more likely than latent or reactivatable proviruses, to integrate in chromatin susceptible for citrullination.

H3cit inhibits latency establishment by interfering with the binding of HP1α to H3K9me3, preventing heterochromatin formation.
Metastatic site influences driver gene function in pancreatic cancer
Authors: Tsanov Kaloyan M. , Barriga Francisco M. , Ho Yu-Jui , Alonso-Curbelo Direna , Livshits Geulah , Koche Richard P. , Baslan Timour , Simon Janelle , Tian Sha , Wuest Alexandra N. , Luan Wei , Wilkinson John E. , Masilionis Ignas , Dimitrova Nevenka , Iacobuzio-Donahue Christine A. , Chaligné Ronan , Pe’er Dana , Massagué Joan , Lowe Scott W.
Published: March 2024

Driver gene mutations can increase the metastatic potential of the primary tumor1–3, but their role in sustaining tumor growth at metastatic sites is poorly understood. A paradigm of such mutations is inactivation ofSMAD4– a transcriptional effector of TGFβ signaling – which is a hallmark of multiple gastrointestinal malignancies4,5.SMAD4inactivation mediates TGFβ’s remarkable anti-to pro-tumorigenic switch during cancer progression and can thus influence both tumor initiation and metastasis6–14. To determine whether metastatic tumors remain dependent onSMAD4inactivation, we developed a mouse model of pancreatic ductal adenocarcinoma (PDAC) that enablesSmad4depletion in the pre-malignant pancreas and subsequentSmad4reactivation in established metastases. As expected,Smad4inactivation facilitated the formation of primary tumors that eventually colonized the liver and lungs. By contrast,Smad4reactivation in metastatic disease had strikingly opposite effects depending on the tumor’s organ of residence: suppression of liver metastases and promotion of lung metastases. Integrative multiomic analysis revealed organ-specific differences in the tumor cells’ epigenomic state, whereby the liver and lungs harbored chromatin programs respectively dominated by the KLF and RUNX developmental transcription factors, withKlf4depletion being sufficient to reverseSmad4’s tumor-suppressive activity in liver metastases. Our results show how epigenetic states favored by the organ of residence can influence the function of driver genes in metastatic tumors. This organ-specific gene–chromatin interplay invites consideration of anatomical site in the interpretation of tumor genetics, with implications for the therapeutic targeting of metastatic disease.
Identification and characterization of neoantigen-reactive CD8+ T cells following checkpoint blockade therapy in a pan-cancer setting
Authors: Moss Keith Henry , Hansen Ulla Kring , de Lima Vinicius Araújo Barosa , Borch Annie , Marquez Esteban Sanchez , Bjerregaard Anne-Mette , Olga Østrup , Bentzen Amalie Kai , Marquard Andrea Marion , kadivar Mohammed , Svane Inge Marie , Lassen Ulrik , Hadrup Sine Reker
Published: March 2024

Background

Immune checkpoint blockade (ICB) has been approved as first-line or second-line therapies for an expanding list of malignancies. T cells recognizing mutation-derived neoantigens are hypothesized to play a major role in tumor elimination. However, the dynamics and characteristics of such neoantigen-reactive T cells (NARTs) in the context of ICB are still limitedly understood.

Methods

To explore this, tumor biopsies and peripheral blood were obtained pre- and post-treatment from 20 patients with solid metastatic tumors, in a Phase I basket trial. From whole-exome sequencing and RNA-seq data, patient-specific libraries of neopeptides were predicted and screened with DNA barcode-labeled MHC multimers for CD8+T cell reactivity, in conjunction with the evaluation of T cell phenotype.

Results

We were able to detect NARTs in the peripheral blood and tumor biopsies for the majority of the patients; however, we did not observe any significant difference between the disease control and progressive disease patient groups, in terms of the breadth and magnitude of the detected NARTs. We also observed that the hydrophobicity of the peptide played a role in defining neopeptides resulting in NARTs response. A trend towards a treatment-induced phenotype signature was observed in the NARTs post-treatment, with the appearance of Ki67+CD27+PD-1+subsets in the PBMCs and CD39+Ki67+TCF-1+subsets in the TILs. Finally, the estimation of T cells from RNAseq was increasing post versus pre-treatment for disease control patients.

Conclusion

Our data demonstrates the possibility of monitoring the characteristics of NARTs from tumor biopsies and peripheral blood, and that such characteristics could potentially be incorporated with other immune predictors to understand further the complexity governing clinical success for ICB therapy.
Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, aLegionella pneumophilaprotein that impinges on a stress response pathway
Authors: Hershkovitz Dar , Chen Emy J. , Ensminger Alexander W. , Dugan Aisling S. , Conway Kaleigh T. , Joyce Alex C. , Segal Gil , Isberg Ralph R.
Published: March 2024

Legionella pneumophilagrows within membrane-bound vacuoles in phylogenetically diverse hosts. Intracellular growth requires the function of the Icm/Dot type-IVb secretion system, which translocates more than 300 proteins into host cells. A screen was performed to identifyL. pneumophilaproteins that stimulate MAPK activation, using Icm/Dot translocated proteins ectopically expressed in mammalian cells. In parallel, a second screen was performed to identifyL. pneumophilaproteins expressed in yeast that cause growth inhibition in MAPK pathway-stimulatory high osmolarity medium. LegA7 was shared in both screens, a protein predicted to be a member of the bacterial cysteine protease family that has five carboxyl-terminal ankyrin repeats. Three conserved residues in the predicted catalytic triad of LegA7 were mutated. These mutations abolished the ability of LegA7 to inhibit yeast growth. To identify other residues important for LegA7 function, a generalizable selection strategy in yeast was devised to isolate mutants that have lost function and no longer cause growth inhibition on high osmolarity medium. Mutations were isolated in the two carboxyl-terminal ankyrin repeats, as well as an inter-domain region located between the cysteine protease domain and the ankyrin repeats. These mutations were predicted by AlphaFold modeling to localize to the face opposite from the catalytic site, arguing that they interfere with the positive regulation of the catalytic activity. Based on our data, we present a model in which LegA7 harbors a cysteine protease domain with an inter-domain and two carboxyl-terminal ankyrin repeat regions that modulate the function of the catalytic domain.
Predicting cell-type-specific exon inclusion in the human brain reveals more complex splicing mechanisms in neurons than glia
Authors: Michielsen Lieke , Hsu Justine , Joglekar Anoushka , Belchikov Natan , Reinders Marcel J.T. , Tilgner Hagen , Mahfouz Ahmed
Published: March 2024

Alternative splicing contributes to molecular diversity across brain cell types. RNA-binding proteins (RBPs) regulate splicing, but the genome-wide mechanisms remain poorly understood. Here, we used RBP binding sites and/or the genomic sequence to predict exon inclusion in neurons and glia as measured by long-read single-cell data in human hippocampus and frontal cortex. We found that alternative splicing is harder to predict in neurons compared to glia in both brain regions. Comparing neurons and glia, the position of RBP binding sites in alternatively spliced exons in neurons differ more from non-variable exons indicating distinct splicing mechanisms. Model interpretation pinpointed RBPs, including QKI, potentially regulating alternative splicing between neurons and glia. Finally, using our models, we accurately predict and prioritize the effect of splicing QTLs. Taken together, our models provide new insights into the mechanisms regulating cell-type-specific alternative splicing and can accurately predict the effect of genetic variants on splicing.
Generalized missing partner mechanism for
SU(5)
GUT inflation
Authors: Antusch Stefan , Hinze Kevin , Saad Shaikh , Steiner Jonathan
Published: March 2024

Embedding inflation within a grand unified theory is an intriguing idea towards a more fundamental understanding of particle physics and cosmology. However, within the framework of supersymmetric SU(5) grand unified theory inflation, models typically feature light color octets and weak triplets that ruin the successful gauge coupling unification of the mimimal supersymmetric Standard Model. We present a new possibility for naturally splitting the electronlike states from the colored ones within vectorlike 10-plets by utilizing their couplings with 40-plets that lack electronlike states. Together with the extra light weak doublets from the double missing partner mechanism, the appearance of the light electronlike states from our proposed generalized missing partner mechanism restores gauge coupling unification. Remarkably, the same vectorlike 10-plets cure the bad mass relations between the down quarks and the charged leptons. Discovering the light relic states at future colliders would provide a “smoking gun” signal of the scenario.

Published by the American Physical Society
2024
Phase field crystal modeling of grain boundary structures in diamond cubic systems
Authors: Blixt Kevin H. , Hallberg Håkan
Published: March 2024

Phase field crystal (PFC) modeling has proved to be a versatile numerical tool in the analysis of crystalline microstructures. Most often, however, the focus is put on bulk crystal behavior, while crystal defects such as grain boundaries (GBs) are less explored. This is, in particular, the case for crystal structures beyond fcc and bcc. In this work, the possibilities and challenges in adopting PFC to diamond cubic (DC) crystal structures is investigated. Three different PFC models are considered for this purpose. One of them was published previously, and two are modifications proposed in the present work. The models are compared in terms of both DC phase stabilization and their ability to provide relevant GB structures. The models employ combinations of two- and three-point correlations, and the addition of a three-point correlation is found to be required for stabilization of the expected DC GB structures. It is concluded that although each of the models has limitations in terms of the GB structures which can be stabilized and performance in terms of phase stability, key PFC components for successful modeling of DC structures can be identified.

Published by the American Physical Society
2024
Stability of long-sustained oscillations induced by electron tunneling
Authors: Tabanera-Bravo Jorge , Vigneau Florian , Monsel Juliette , Aggarwal Kushagra , Bresque Léa , Fedele Federico , Cerisola Federico , Briggs G. A. D. , Anders Janet , Auffèves Alexia , Parrondo Juan M. R. , Ares Natalia
Published: March 2024

Self-oscillations are the result of an efficient mechanism generating periodic motion from a constant power source. In quantum devices, these oscillations may arise due to the interaction between single electron dynamics and mechanical motion. We show that, due to the complexity of this mechanism, these self-oscillations may irrupt, vanish, or exhibit a bistable behavior causing hysteresis cycles. We observe these hysteresis cycles and characterize the stability of different regimes in both single- and double-quantum-dot configurations. In particular cases, we find these oscillations stable for over 20 s, many orders of magnitude above electronic and mechanical characteristic timescales, revealing the robustness of the mechanism at play.

Published by the American Physical Society
2024
Interspecies Förster resonances for Rb-Cs Rydberg
d
-states for enhanced multi-qubit gate fidelities
Authors: Ireland P. M. , Walker D. M. , Pritchard J. D.
Published: March 2024

We present an analysis of interspecies interactions between Rydberg d-states of rubidium and cesium. We identify the Förster resonance channels offering the strongest interspecies couplings, demonstrating the viability for performing high-fidelity two- and multi-qubit CkZ gates up to k=4, including accounting for blockade errors evaluated via numerical diagonalization of the pair potentials. Our results show d-state orbitals offer enhanced suppression of intraspecies couplings compared to s-states, making them well suited for use in large-scale neutral atom quantum processors.

Published by the American Physical Society
2024
Quantum interference between distant creation processes
Authors: Pseiner Johannes , Erhard Manuel , Krenn Mario
Published: March 2024

The search for macroscopic quantum phenomena is a fundamental pursuit in quantum mechanics. It allows us to test the limits of quantum physics and provides new avenues for exploring the interplay between quantum mechanics and relativity. In this work, we introduce a novel approach to generate macroscopic quantum systems by demonstrating that the creation process of a quantum system can span a macroscopic distance. Specifically, we generate photon pairs in a coherent superposition of two origins separated by up to 70 meters. This new approach not only provides an exciting opportunity for foundational experiments in quantum physics, but also has practical applications for high-precision measurements of distributed properties such as pressure and humidity of air or gases.

Published by the American Physical Society
2024
The single-atom R1: a new optimization method to solve crystal structures
Authors: Zhang Xiaodong , Donahue James P.
Published: March 2024

A crystal structure with N atoms in its unit cell can be solved starting from a model with atoms 1 to j − 1 being located. To locate the next atom j, the method uses a modified definition of the traditional R1 factor where its dependencies on the locations of atoms j + 1 to N are removed. This modified R1 is called the single-atom R1 (sR1), because the locations of atoms 1 to j − 1 in sR1 are the known parameters, and only the location of atom j is unknown. Finding the correct position of atom j translates thus into the optimization of the sR1 function, with respect to its fractional coordinates, x

j
, y

j
, zj
. Using experimental data, it has been verified that an sR1 has a hole near each missing atom. Further, it has been verified that an algorithm based on sR1, hereby called the sR1 method, can solve crystal structures (with up to 156 non-hydrogen atoms in the unit cell). The strategy to carry out this calculation has also been optimized. The main feature of the sR1 method is that, starting from a single arbitrarily positioned atom, the structure is gradually revealed. With the user's help to delete poorly determined parts of the structure, the sR1 method can build the model to a high final quality. Thus, sR1 is a viable and useful tool for solving crystal structures.
Leadership behavioural integrity and employee engagement: role of organisational career development and feedback self-efficacy
Authors: Jena Lipsa , Pattnaik Subash Chandra , Sahoo Rashmita
Published: March 2024

Purpose

The present study purports to unravel the mechanism in relationship among leadership behaviour integrity, organisational career development and employee engagement. Further, it also aims to understand if the employee feedback self-efficacy has any moderating influence on the relationship between leader behavioural integrity and organisational career development.

Design/methodology/approach

Pre-existing questionnaires are used for collecting data from a total of 417 employees working in the information technology industry operating within India. Analysis of the data is done using structural equation modelling technique.

Findings

Results of the study show that organisational career development partially mediates the relationship between leadership behavioural integrity and employee engagement. It is also found that feedback self-efficacy plays a moderating role in the relationship between leadership behavioural integrity and organisational career development.

Originality/value

The study helps to understand the mechanism of the relationship between leadership behavioural integrity and employee engagement through organisational career development with the support of ethical theory and social exchange theory. It also shows the moderating role played by feedback self-efficacy in the relationship between leadership behavioural integrity and organisational career development using social learning perspective.
The impact of the collaborative consumption triangle on purchasing intentions within online food delivery platforms
Authors: Cheng Fei-Fei , Hsu Meng-Hsu , Wu Chin-Shan
Published: March 2024

Purpose

This study adopted the collaborative consumption triangle to explore the influence of online food delivery platforms (OFDP) on consumer purchase intentions. It investigates the effects of restaurants' corporate social responsibility (CSR) practices, individuals' food neophilic tendencies (FNT), and platforms' perceived benefits on purchase intention within OFDP. Furthermore, the study analyses differences in consumers' pro-environmental behaviour (PEB) on OFDP.

Design/methodology/approach

The 497 participants conducted a web-based self-completion survey, using structural equation modelling to analyse the path structure of consumer purchasing intention. Furthermore, differences in PEB among OFDP consumers were compared through multigroup analysis.

Findings

The findings indicate that CSR influences the perceived value of sustainability and that the perceived value of sustainability influences purchase intention. Additionally, the influence of the perceived value of sustainability on purchase intention is more pronounced among consumers with low PEB compared to those with high PEB.

Research limitations/implications

The findings may not be generalisable to other countries due to cultural differences, CSR policies, and strategies for promoting sustainable development.

Social implications

The study provides valuable contributions related to (1) restaurants increasing their revenue and meeting their long-term sustainable development goals; (2) providing reusable containers policy and reusable containers policy and category tags for restaurants within OFDP.

Originality/value

This study is a pioneering work examining factors influencing purchase intentions within OFDP from the tripartite collaborative consumption perspective post-COVID-19 and focuses on the differences in PEB concerning OFDP.
Impact of challenge and hindrance job stressors on informal safety communication of construction workers in China: the moderating role of co-worker relationship
Authors: Cong Weiyi , Zhang Shoujian , Liang Huakang , Xiang Qingting
Published: March 2024

Purpose

Job stressors have a considerable influence on workplace safety behaviors. However, the findings from previous studies regarding the effect of different types of job stressors have been contradictory. This is attributable to, among other factors, the effectiveness of job stressors varying with occupations and contexts. This study examines the effects of challenge and hindrance stressors on construction workers' informal safety communication at different levels of coworker relationships.

Design/methodology/approach

A three-dimensional framework of informal safety communication is adopted, including self-needed, citizenship and participatory safety communication. Stepwise regression analysis is then performed using questionnaire survey data collected from 293 construction workers in the Chinese construction industry.

Findings

The results demonstrate that both challenge and hindrance stressors are negatively associated with self-needed and citizenship safety communication, whereas their relationships with participatory safety communication are not significant. Meanwhile, the mitigation effects of the coworker relationship (represented by trustworthiness and accessibility) on the above negative impacts vary with the communication forms. Higher trustworthiness and accessibility enable workers faced with challenge stressors to actively manage these challenges and engage in self-needed safety communication. Similarly, trustworthiness promotes workers' involvement in self-needed and citizenship safety communication in the face of hindrance stressors, but accessibility is only effective in facilitating self-needed safety communication.

Originality/value

By introducing the job demands-resources theory and distinguishing informal safety communication into three categories, this study explains the negative effects of challenge and hindrance job stressors in complex and variable construction contexts and provides additional clues to the current inconsistent findings regarding this framework. The diverse roles of challenge and hindrance job stressors also present strong evidence for the need to differentiate between the types of informal safe communication.
A content analysis of the frequency of fat talk in Walt Disney animation films (1937–2021)
Authors: Ogden Jane , Chohan Alissa
Published: March 2024

Purpose

Previous research demonstrates a consistent association between the media and body and eating related issues in children. Recent research has highlighted a role for “fat talk” to describe discourses around body size and food. One key source of media information is Disney animation films, yet to date no research study has explored the verbal content of this genre.

Design/methodology/approach

The present study used a content analysis to examine fat talk in Disney animation films (1937–2021; n = 53) with a focus on the frequency of fat talk, changes over time and differences between the genders and heroic statuses of the givers and receivers of fat talk. Fat talk was defined as relating to both body size and food and could be either positive or negative.

Findings

Results revealed that there was more negative than positive fat talk per film; no significant changes over time; males were the givers of significantly more positive and negative fat talk than females and were also the receivers of more negative fat talk; good characters were the givers and receivers of more positive and negative fat talk and more self-directed negative fat talk than bad characters.

Practical implications

The results are discussed in terms of possible legislation and parenting interventions to minimise the harm of this genre on young children.

Originality/value

Disney animation films may not be as benign as often thought.
The effect of sovereign credit ratings on democracy in sub-Saharan Africa
Authors: Gossel Sean , Mutize Misheck
Published: March 2024

Purpose

This study investigates (1) whether democratization drives sovereign credit ratings (SCR) changes (the “democratic advantage”) or whether SCR changes affect democratization, (2) whether the degree of democratization in sub-Saharan African (SSA) countries affects the associations and (3) whether the associations are significantly affected by resource dependence.

Design/methodology/approach

This study investigates the effects of SCR changes on democracy in 22 SSA countries over the period of 2000–2020 VEC Granger causality/block exogeneity Wald tests, and impulse responses and variance decomposition analyses with Cholesky ordering and Monte Carlo standard errors in a panel VECM framework.

Findings

The full sample impulse responses find that a SCR shock has a long-run detrimental effect on the democracy and political rights but only a short-run positive impact on civil liberties. Among the sub-samples, it is found that the extent of natural resource dependence does not affect the magnitude of SCR shocks on democratization mentioned above but it is found that a SCR shock affects long-run democracy in SSA countries that are relatively more democratic but is more likely to drive democratic deepening in less democratic SSA countries. The full sample variance decompositions further finds that the variance of SCR to a political rights shock outweighs the effects of all the macroeconomic factors, whereas in more diversified SSA countries, the variances of SCR are much greater for democracy and political rights shocks, which suggests that democratization and political rights in diversified SSA economies are severely affected by SCR changes. In the case of the high and low democracy sub-samples, it is found that the variance of SCR in the relatively higher democracy sub-sample is greater than in the low democracy sub-sample.

Social implications

These results have three implications for democratization in SSA. First, the effect of a SCR change is not a democratically agnostic and impacts political rights to a greater extent than civil liberties. Second, SCR changes have the potential to spark a negative cycle in SSA countries whereby a downgrade leads to a deterioration in socio-political stability coupled with increased financial economic constraints that in turn drive further downgrades and macroeconomic hardship. Finally, SCR changes are potentially detrimental for democracy in more democratic SSA countries but democratically supportive in less democratic SSA countries. Thus, SSA countries that are relatively politically sophisticated are more exposed to the effects of SCR changes, whereas less politically sophisticated SSA countries can proactively shape their SCRs by undertaking political reforms.

Originality/value

This study is the first to examine the associations between SCR and democracy in SSA. This is critical literature for the Africa’s scholarly work given that the debate on unfair rating actions and claims of subjective rating methods is ongoing.
Static and dynamic characteristics of large-span six-slider closed hydrostatic guideway considering pitch moment and yaw moment
Authors: Zhang Heji , Lu Dezhao , Pan Wei , Rong Xing , Zhang Yongtao
Published: March 2024

Purpose

The purpose of this study is to design a closed hydrostatic guideway has the ability to resist large-side load, pitch moments and yaw moments, has good stiffness and damping characteristics, and provides certain beneficial guidance for the design of large-span closed hydrostatic guideway on the basis of providing a large vertical load bearing capacity.

Design/methodology/approach

The Reynolds’ equation and flow continuity equation are solved simultaneously by the finite difference method, and the perturbation method and the finite disturbance method is used for calculating the dynamic characteristics. The static and dynamic characteristics, including recess pressure, flow of lubricating oil, carrying capacity, pitch moment, yaw moment, dynamic stiffness and damping, are comprehensively analyzed.

Findings

The designed closed hydrostatic guideway has the ability to resist large lateral load, pitch moment and yaw moment and has good stiffness and damping characteristics, on the basis of being able to provide large vertical carrying capacity, which can meet the application requirements of heavy two-plate injection molding machine (TPIMM).

Originality/value

This paper researches static and dynamic characteristics of a large-span six-slider closed hydrostatic guideway used in heavy TPIMM, emphatically considering pitch moment and yaw moment. Some useful guidance is given for the design of large-span closed hydrostatic guideway.
Development of a novel nonrigid support friction stir welding repair robot for aluminum alloy train
Authors: Jin Taotao , Cui Xiuhui , Qi Chuanyue , Yang Xinyu
Published: March 2024

Purpose

This paper aims to develop a specific type of mobile nonrigid support friction stir welding (FSW) robot, which can adapt to aluminum alloy trucks for rapid online repair.

Design/methodology/approach

The friction stir welding robot is designed to complete online repair according to the surface damage of large aluminum alloy trucks. A rotatable telescopic arm unit and a structure for a cutting board in the shape of a petal that was optimized by finite element analysis are designed to give enough top forging force for welding to address the issues of inadequate support and significant deformation in the repair process.

Findings

The experimental results indicate that the welding robot is capable of performing online surface repairs for large aluminum alloy trucks without rigid support on the backside, and the welding joint exhibits satisfactory performance.

Practical implications

Compared with other heavy-duty robotic arms and gantry-type friction stir welding robots, this robot can achieve online welding without disassembling the vehicle body, and it requires less axial force. This lays the foundation for the future promotion of lightweight equipment.

Originality/value

The designed friction stir welding robot is capable of performing online repairs without dismantling the aluminum alloy truck body, even in situations where sufficient upset force is unavailable. It ensures welding quality and exhibits high efficiency. This approach is considered novel in the field of lightweight online welding repairs, both domestically and internationally.
Rich alone or rich together: the impact of value proposition innovation on the product–place co-branding value
Authors: Zhu Rong , Fu Yaoyao , Wen Ao , Zhao Jiaxin
Published: March 2024

Purpose

This study aims to examine an emerging product–place co-branding marketing practice in China’s rural areas. The role of this practice in inclusive development is analyzed from the perspectives of value proposition innovation, market legitimacy, media coverage and brand value. Both research and practice indicate value proposition innovation to exert an important influence on brand value enhancement, but little is known about the mediating and moderating mechanisms underlying this relation.

Design/methodology/approach

A moderated mediation model is constructed to examine whether market legitimacy mediates the relationship between value proposition innovation and brand value. vWhether this mediating process is moderated by media coverage is also examined. The primary data are collected from semi-structured interviews and observations conducted with two common cases to develop proper scales for value proposition innovation and market legitimacy. The research includes 100 product–place co-brandings published by the Ministry of Agriculture and Rural Affairs in 2019. Hypotheses are tested using hierarchical regression and a Bootstrap model.

Findings

Value proposition innovation has a positive effect on brand value, and market legitimacy partially mediates this relationship. Media coverage positively moderates the relationship between value proposition innovation and market legitimacy, and positively moderates the mediating effect of market legitimacy; the higher the media coverage, the stronger the mediating effect of market legitimacy.

Research limitations/implications

Based on data availability and accessibility, the study sample focused on indicators from 100 brands in 2019. If the Ministry of Agriculture and Rural Affairs discloses consecutive annual information for other years, future studies could explore panel data to further test the study’s conclusions from a longitudinal perspective.

Originality/value

First, this paper adds to the emerging literature on product–place co-branding business models by examining the relationship between value proposition innovation and brand value. Second, this paper enriches institutional theory by including market legitimacy as a mediator between value proposition innovation and brand value. Third, this paper identifies the moderating role of media coverage, thus broadening the theoretical implications of institutional theory with respect to improving market legitimacy.
Foreign direct investment and carbon emissions in ECOWAS: does good governance matter?
Authors: Limazie Mazignada Sika , Woni Soumaïla
Published: March 2024

Purpose

The present study investigates the effect of foreign direct investment (FDI) and governance quality on carbon emissions in the Economics Community of West African States (ECOWAS).

Design/methodology/approach

To achieve the objective of this research, panel data for dependent and explanatory variables over the period 2005–2016, collected in the World Development Indicators (WDI) database and World Governance Indicators (WGI), are analyzed using the generalized method of moments (GMM). Also, the panel-corrected standard errors (PCSE) method is applied to the four segments of the overall sample to analyze the stability of the results.

Findings

The findings of this study are (1) FDI inflows have a negative effect on carbon emissions in ECOWAS and (2) The interaction between FDI inflows and governance quality have a negative effect on carbon emissions. These results show the decreasing of environmental damage by increasing institutional quality. However, the estimation results on the country subsamples show similar and non-similar aspects.

Practical implications

This study suggests that policymakers in the ECOWAS countries should strengthen their environmental policies while encouraging FDI flows to be environmentally friendly.

Originality/value

The subject has rarely been explored in West Africa, with gaps such as the lack of use of institutional variables. This study contributes to the literature by drawing on previous work to examine the role of good governance on FDI and the CO2 emission relationship in the ECOWAS, which have received little attention. However, this research differs from previous work by subdividing the overall sample into four groups to test the stability of the results.
Vertical policy coordination of COVID-19 testing in Sweden: an analysis of policy-specific demands and institutional barriers
Authors: Hallberg Anna , Winblad Ulrika , Fredriksson Mio
Published: March 2024

Purpose

The build-up of large-scale COVID-19 testing required an unprecedented effort of coordination within decentralized healthcare systems around the world. The aim of the study was to elucidate the challenges of vertical policy coordination between non-political actors at the national and regional levels regarding this policy issue, using Sweden as our case.

Design/methodology/approach

Interviews with key actors at the national and regional levels were analyzed using an adapted version of a conceptualization by Adam et al. (2019), depicting barriers to vertical policy coordination.

Findings

Our results show that the main issues in the Swedish context were related to parallel sovereignty and a vagueness regarding responsibilities and mandates as well as complex governmental structures and that this was exacerbated by the unfamiliarity and uncertainty of the policy issue. We conclude that understanding the interaction between the comprehensiveness and complexity of the policy issue and the institutional context is crucial to achieving effective vertical policy coordination.

Originality/value

Many studies have focused on countries’ overall pandemic responses, but in order to improve the outcome of future pandemics, it is also important to learn from more specific response measures.
Online brand community engagement and brand evangelism: the role of age, gender and membership number
Authors: Bhandari Min Prasad , Bhattarai Charan , Mulholland Gary
Published: March 2024

Purpose

This study aims to investigate the critical role of online brand community (OBC) engagement and brand evangelism: the role of age, gender and membership number.

Design/methodology/approach

To achieve the study’s objective, 303 active Facebook OBC users were surveyed. The AMOS structural equation modelling (SEM) is used to test the hypothesised model.

Findings

The results indicate that OBC engagement improves brand evangelism. Specifically, the results show that age, gender, and OBC membership number moderate the relationship between OBC engagement and brand evangelism. Interestingly, this study found that female, younger and low-OBC follower consumers more significantly contribute to nourishing brand evangelism than male, older and high-OBC follower counterparts.

Originality/value

OBC engagement is validated as a key brand evangelism driver, further substantiating its role as a crucial strategic metric. Moreover, age, gender and OBC membership number as moderating factors in the association between OBC engagement and brand evangelism (word of mouth referral, brand defence and future purchases) have been verified. Although the findings suggest that improved OBC engagement contributes to evangelism, this effect transpires more significantly among female, younger and low-OBC followers than male, older and high-OBC followers.
Commitment to and connection with green brands: perspectives of consumer social responsibility and terror management theory
Authors: Rahimah Anni , Do Ben-Roy , Le Angelina Nhat Hanh , Cheng Julian Ming Sung
Published: March 2024

Purpose

This study aims to investigate specific green-brand affect in terms of commitment and connection through the morality–mortality determinants of consumer social responsibility and the assumptions of terror management theory in the proposed three-layered framework. Religiosity serves as a moderator within the framework.

Design/methodology/approach

Data are collected in Taipei, Taiwan, while quota sampling is applied, and 420 valid questionnaires are collected. The partial least squares technique is applied for data analysis.

Findings

With the contingent role of religiosity, consumer social responsibility influences socially conscious consumption, which in turn drives the commitment and connection of green-brand affect. The death anxiety and self-esteem outlined in terror management theory influence materialism, which then drives green-brand commitment; however, contrary to expectations, they do not drive green-brand connection.

Originality/value

By considering green brands beyond their cognitive aspects and into their affective counterparts, morality–mortality drivers of green-brand commitment and green-grand connection are explored to provide unique contributions so as to better understand socially responsible consumption.
Sodium and potassium ferrocyanide derived Prussian blue pigment
Authors: Sanghvi Malav R. , Chugh Karan W. , Mhaske S.T.
Published: March 2024

Purpose

This study aims to synthesize Prussian blue {FeIII4[FeII(CN)6]3} pigment by reacting ferric chloride with different ferrocyanides through the same procedure. The influence of the ferrocyanide used on resulting pigment properties is studied.

Design/methodology/approach

Prussian blue is commonly synthesized by direct or indirect methods, through iron salt and ferrocyanide/ferricyanide reactions. In this study, the direct, single-step process was pursued by dropwise addition of the ferrocyanide into ferric chloride (both as aqueous solutions). Two batches – (K-PB) and (Na-PB) – were prepared by using potassium ferrocyanide and sodium ferrocyanide, respectively. The development of pigment was confirmed by an identification test and characterized by spectroscopic techniques. Pigment properties were determined, and light fastness was observed for acrylic emulsion films incorporating dispersed pigment.

Findings

The two pigments differed mainly in elemental detection owing to the dissimilar ferrocyanide being used; IR spectroscopy where only (Na-PB) showed peaks indicating water molecules; and bleeding tendency where (K-PB) was water soluble whereas (Na-PB) was not. The pigment exhibited remarkable blue colour and good bleeding resistance in several solvents and showed no fading in 24 h of light exposure though oil absorption values were high.

Originality/value

This article is a comparative study of Prussian blue pigment properties obtained using different ferrocyanides. The dissimilarity in the extent of water solubility will influence potential applications as a colourant in paints and inks. K-PB would be advantageous in aqueous formulations to confer a blue colour without any dispersing aid but unfavourable in systems where other coats are water-based due to their bleeding tendency.
Perception of employees towards learning in hybrid workplace: a study of university faculty
Authors: Khatun Ayesha , Singh Vishal , Joshi Akashdeep
Published: March 2024

Purpose

Studies have so far focused on learning in organizations, factors affecting learning, learning effectiveness and so on but the concept of learning in a hybrid work arrangement is yet unexplored. The purpose of this study is to measure the perception of faculty members in higher education institutions towards learning in a hybrid work arrangement and also to measure the differences of perception towards hybrid work arrangement based on employees’ gender and organization type.

Design/methodology/approach

The data was collected from a sample of 390 faculty members composing of Assistant Professors, Associate Professors and Professors, purposely chosen from two of the premier higher education institutions (one private and one public) located in Punjab, India. A self-structured questionnaire was administered to the faculty members who are working on a regular basis and have minimum of two years of work experience with the chosen university. For analysing the collected data exploratory factor analysis and other descriptive statistics have been applied.

Findings

The findings of the survey show that in terms of gender differences, it is the female employees who are more satisfied with different aspects of hybrid/remote work arrangement as compared to male employees. In regard to organizational differences in the perception towards learning in a hybrid work arrangement it is found that public university employees have a more positive attitude so far as individual factors are concerned, but in terms of organizational factors, it is the private university that is scoring better than the public university.

Research limitations/implications

The study is limited to only two higher education institutions, and its findings to be applicable in all higher education institutions, further studies may be required on a larger canvas. Future studies may be undertaken using advanced statistical tools like structural equation modelling to explore various variables associated with learning in a hybrid work arrangement.

Originality/value

Applicability of hybrid work arrangement is very high in higher education institutions and to the best of the authors’ knowledge, this is the first study which adds to the literature on perception of employees towards organizational learning in a hybrid work arrangement.
Stand-alone PV-SOFC-battery power system based on ANFIS controller for electric vehicle charging
Authors: Khan Naseer , Gohar Zeeshan , Khan Faisal , Mehmood Faisal
Published: March 2024

Purpose

This study aims to offer a hybrid stand-alone system for electric vehicle (EV) charging stations (CS), an emerging power scheme due to the availability of renewable and environment-friendly energy sources. This paper presents the analysis of a photovoltaic (PV) with an adaptive neuro-fuzzy inference system (ANFIS) algorithm, solid oxide fuel cell (SOFC) and a battery storage scheme incorporated for EV CS in a stand-alone mode. In previous studies, either the hydrogen fuel of SOFC or the irradiance is controlled using artificial neural network. These parameters are not controlled simultaneously using an ANFIS-based approach. The ANFIS-based stand-alone hybrid system controlling both the fuel flow of SOFC and the irradiance of PV is discussed in this paper.

Design/methodology/approach

The ANFIS algorithm provides an efficient estimation of maximum power (MP) to the nonlinear voltage–current characteristics of a PV, integrated with a direct current–direct current (DC–DC) converter to boost output voltage up to 400 V. The issue of fuel starvation in SOFC due to load transients is also mitigated using an ANFIS-based fuel flow regulator, which robustly provides fuel, i.e. hydrogen per necessity. Furthermore, to ensure uninterrupted power to the CS, PV is integrated with a SOFC array, and a battery storage bank is used as a backup in the current scenario. A power management system efficiently shares power among the aforesaid sources.

Findings

A comprehensive simulation test bed for a stand-alone power system (PV cells and SOFC) is developed in MATLAB/Simulink. The adaptability and robustness of the proposed control paradigm are investigated through simulation results in a stand-alone hybrid power system test bed.

Originality/value

The simulation results confirm the effectiveness of the ANFIS algorithm in a stand-alone hybrid power system scheme.