bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of Sound Therapy for Tinnitus Using an Enriched Acoustic Environment with Hearing-Loss Matched Broadband Noise
Authors: Cuesta María , Garzón Christiam , Cobo Pedro
Published: January 2022

Background: Tinnitus is a rather heterogeneous chronic condition/disorder which is difficult to treat. Some tinnitus treatments combine sound therapy with counselling. The main goal of this study is to report the efficacy of a customized sound therapy combined with counselling on a cohort of 83 tinnitus patients. Methods: 119 tinnitus subjects, recruited between January 2018 and June 2021, were subjected to a treatment consisting of a combination of an initial counselling session and four-month sound therapy. The sound stimulus was a personalized broadband noise colored by the audiometry of the subjects. These stimuli were given to the patients in mp3 format to be heard 1 h per day over 4 months. The tinnitus severity of the patients was evaluated monthly through the validated Spanish version of the Tinnitus Handicap Inventory. Results: Of the patients, 30% (36 of 119) withdrew from the treatment before finishing, and 96% (80 of 83) of the subjects completing the therapy attained some relief after 4 months. The overall average THI decrease of these 80 participants was 23. However, when the THI was analyzed by severity scales, it was found that patients with initial mild, moderate, severe and catastrophic handicap had an average THI decrease of 14, 20, 31 and 42 points, respectively. Thus, the average THI decrease depended on the baseline severity scale of patients. Conclusions: Consequently, the proposed treatment was demonstrated to be effective in providing clinically relevant relief in tinnitus distress patients in just 4 months.
A Systematic Review on Supply Chain Management in Prefabricated House-Building Research
Authors: Masood Rehan , Lim James , González Vicente , Roy Krishanu , Khan Khurram
Published: January 2022

Prefabricated house-building companies, as suppliers or supply chains, which use manufacturing as a business approach towards industrialization, struggle to implement principles and optimal practices driven from well-established and validated theories in operational research. Supply chain management has a mature body of knowledge that has been widely adopted by research on offsite construction to improve its performance at an organisational level. However, there is no comprehensive review available in the literature for supply chain management theory within prefabricated house building research from the perspective of suppliers. In this study, a systematic review was conducted on the available literature on supply chain management within prefabricated house-building research. Initially, qualitative analysis was performed to identify the key themes. Later, quantitative analyses were applied to validate the overlapping themes and keywords. Further, key trends related to focus, methods and theories or frameworks were reported. The findings were discussed in the context of recent developments in all principal component bodies of supply chain management for future work. This study also provides a brief guide for potential future review studies to explore interdisciplinary intervention within the offsite stream.
Post-Earthquake Rapid Damage Assessment of Road Bridges in Glina County
Authors: Vlašić Anđelko , Srbić Mladen , Skokandić Dominik , Mandić Ivanković Ana
Published: January 2022

In December 2020, a strong earthquake occurred in Northwestern Croatia with a magnitude of ML = 6.3. The epicenter of this earthquake was located in the town of Petrinja, about 50 km from Zagreb, and caused severe structural damage throughout Sisak-Moslavina county. One of the biggest problems after this earthquake was the structural condition of the bridges, especially since most of them had to be used immediately for demolition, rescue, and the transport of mobile housing units in the affected areas. Teams of civil engineers were quickly formed to assess the damage and structural viability of these bridges and take necessary actions to make them operational again. This paper presents the results of the rapid post-earthquake assessment for a total of eight bridges, all located in or around the city of Glina. For the assessment, a visual inspection was performed according to a previously established methodology. Although most of the inspected bridges were found to be deteriorated due to old age and lack of maintenance, very few of them showed serious damage from the earthquake, with only one bridge requiring immediate strengthening measures and use restrictions. These measurements are also presented in this paper.
Investigation on Mechanical and Durability Properties of Concrete Mixed with Silica Fume as Cementitious Material and Coal Bottom Ash as Fine Aggregate Replacement Material
Authors: Ali Tariq , Buller Abdul Salam , Abro Fahad ul Rehman , Ahmed Zaheer , Shabbir Samreen , Lashari Ali Raza , Hussain Ghulam
Published: January 2022

Cement production produces a high amount of carbon dioxide, which has a negative impact on the environment. By utilizing waste products instead of cement, environmental degradation can be reduced. The current study was undertaken to study the mechanical and durability performance of concrete by replacing 7.5%, 10%, and 12.5% silica fume (SF) of cement weight. Additionally, coal bottom ash (CBA) was also substituted as fine aggregates with 10%, 20%, and 30%. Compressive strength and indirect tensile strength were the major parameters regarding mechanical properties, while corrosion analysis and sulfate attack were set for durability performance. Sixteen mixes were prepared including a control mix. Out of these, three mixes contained SF, three mixes contained CBA, and eight mixes contained both SF and CBA with 1:2:4 ratio at 0.5 w/b ratio. The results concluded that the addition of 12.5% SF and 30% CBA gives optimum compressive strength and tensile strength. Furthermore, using the SF and CBA reduces the workability of concrete. Furthermore, the use of these byproducts increased the durability in terms of corrosion and sulfate attack.
Water Consumption and Environmental Impact of Multifamily Residential Buildings: A Life Cycle Assessment Study
Authors: Mannan Mehzabeen , Al-Ghamdi Sami G.
Published: January 2022

Water use in buildings accounts for a large share in global freshwater consumption where research on the impacts of life cycle water use receive little or no attention. Moreover, there is very limited knowledge regarding such impacts that focus on the life cycle emissions from water consumption in building environments in the world’s most water-stressed countries. Hence, this study attempted to quantify the environmental impacts of operational water use in a multi-family residential building through a life cycle assessment (LCA). A small part of a Middle Eastern country, Doha (Qatar), has been selected for the primary assessment, while water-use impact in Miami (Florida) was chosen as a second case study, as both locations fall into similar climate zone according to ASHRAE Climate Zone Map. The LCA score indicated much higher impacts in the Doha case study compared to Miami. The variation in the result is mainly attributed to the raw water treatment stage in Doha, which involves energy-intensive thermal desalination. Again, relative comparison of the annual water and electricity use impacts for the modeled building was performed at the final stage for both locations. Water use was attributable for 18% of the environmental impacts in Miami, while this value increased to 35% in Doha. This initial assembled LCA result will be beneficial to both water authorities and building research communities in establishing more sustainable water use policies for specific regions/countries that will ultimately benefit the overall building environment.
A Comparative Study on the Life Cycle Assessment of New Zealand Residential Buildings
Authors: Dani Aflah Alamsah , Roy Krishanu , Masood Rehan , Fang Zhiyuan , Lim James B. P.
Published: January 2022

In New Zealand, housing is typically low density, with light timber framing being the dominant form of construction with more than 90% of the market. From 2020, as a result of the global pandemic, there was a shortage of timber in New Zealand, resulting in increased popularity for light steel framing, the main alternative to timber for housing. At the same time, the New Zealand government is committed to sustainability practises through legislation and frameworks, such as the reduction of whole-of-life carbon emissions for the building industry. New Zealand recently announced reducing its net greenhouse gas emissions by 50% within 2030. Life cycle assessment (LCA) is a technique for assessing the environmental aspects associated with a product over its life cycle. Despite the popularity of LCA in the construction industry of New Zealand, prior research results seem varied. There is no unified NZ context database to perform an LCA for buildings. Therefore, in this paper, a comprehensive study using LCA was conducted to quantify and compare the quantity of carbon emissions from two commonly designed houses in the Auckland region, one built from light timber and the other from light steel, both designed for a lifespan of 90 years. The cradle-to-cradle system boundary was used for the LCA. From the results of this study, it was found that the light steel house had 12.3% more carbon in total (including embodied and operational carbons) when compared to the light timber house, of which the manufacturing of two houses had a difference of 50.4% in terms of carbon emissions. However, when the end-of-life (EOL) analysis was included, it was found that the extra carbon could be offset due to the steel’s recyclability, reducing the amount of embodied carbon in the manufacturing process. Therefore, there was no significant difference in carbon emissions between the light steel and the light timber building, with the difference being only 12.3%.
Implication of Ceramide Kinase/C1P in Cancer Development and Progression
Authors: Camacho Laura , Ouro Alberto , Gomez-Larrauri Ana , Carracedo Arkaitz , Gomez-Muñoz Antonio
Published: January 2022

Cancer cells rewire their metabolic programs to favor biological processes that promote cell survival, proliferation, and dissemination. Among this relevant reprogramming, sphingolipid metabolism provides metabolites that can favor or oppose these hallmarks of cancer. The sphingolipid ceramide 1-phosphate (C1P) and the enzyme responsible for its biosynthesis, ceramide kinase (CERK), are well established regulators of cell growth and survival in normal, as well as malignant cells through stress-regulated signaling pathways. This metabolite also promotes cell survival, which has been associated with the feedback regulation of other antitumoral sphingolipids or second messengers. C1P also regulates cancer cell invasion and migration of different types of cancer, including lung, breast, pancreas, prostate, or leukemia cells. More recently, CERK and C1P have been implicated in the control of inflammatory responses. The present review provides an updated view on the important role of CERK/C1P in the regulation of cancer cell growth, survival, and dissemination.
Delta-Radiomics Predicts Response to First-Line Oxaliplatin-Based Chemotherapy in Colorectal Cancer Patients with Liver Metastases
Authors: Giannini Valentina , Pusceddu Laura , Defeudis Arianna , Nicoletti Giulia , Cappello Giovanni , Mazzetti Simone , Sartore-Bianchi Andrea , Siena Salvatore , Vanzulli Angelo , Rizzetto Francesco , Fenocchio Elisabetta , Lazzari Luca , Bardelli Alberto , Marsoni Silvia , Regge Daniele
Published: January 2022

The purpose of this paper is to develop and validate a delta-radiomics score to predict the response of individual colorectal cancer liver metastases (lmCRC) to first-line FOLFOX chemotherapy. Three hundred one lmCRC were manually segmented on both CT performed at baseline and after the first cycle of first-line FOLFOX, and 107 radiomics features were computed by subtracting textural features of CT at baseline from those at timepoint 1 (TP1). LmCRC were classified as nonresponders (R−) if they showed progression of disease (PD), according to RECIST1.1, before 8 months, and as responders (R+), otherwise. After feature selection, we developed a decision tree statistical model trained using all lmCRC coming from one hospital. The final output was a delta-radiomics signature subsequently validated on an external dataset. Sensitivity, specificity, positive (PPV), and negative (NPV) predictive values in correctly classifying individual lesions were assessed on both datasets. Per-lesion sensitivity, specificity, PPV, and NPV were 99%, 94%, 95%, 99%, 85%, 92%, 90%, and 87%, respectively, in the training and validation datasets. The delta-radiomics signature was able to reliably predict R− lmCRC, which were wrongly classified by lesion RECIST as R+ at TP1, (93%, averaging training and validation set, versus 67% of RECIST). The delta-radiomics signature developed in this study can reliably predict the response of individual lmCRC to oxaliplatin-based chemotherapy. Lesions forecasted as poor or nonresponders by the signature could be further investigated, potentially paving the way to lesion-specific therapies.
Classic Hodgkin Lymphoma Refractory for ABVD Treatment Is Characterized by Pathologically Activated Signal Transduction Pathways as Revealed by Proteomic Profiling
Authors: Honoré Bent , Andersen Maja Dam , Wilken Diani , Kamper Peter , d’Amore Francesco , Hamilton-Dutoit Stephen , Ludvigsen Maja
Published: January 2022

In classic Hodgkin lymphoma (cHL), the tumour microenvironment (TME) is of major pathological relevance. The paucity of neoplastic cells makes it important to study the entire TME when searching for prognostic biomarkers. Cure rates in cHL have improved markedly over the last several decades, but patients with primary refractory disease still show inferior survival. We performed a proteomic comparison of pretreatment tumour tissue from ABVD treatment-refractory versus ABVD treatment-sensitive cHL patients, in order to identify biological differences correlating with treatment outcome. Formalin-fixed paraffin-embedded tumour tissues from 36 patients with cHL, 15 with treatment-refractory disease, and 21 with treatment-sensitive disease, were processed for proteomic investigation. Label-free quantification nano liquid chromatography tandem mass spectrometry was performed on the tissues. A total of 3920 proteins were detected and quantified between the refractory and sensitive groups. This comparison revealed several subtle but significant differences in protein expression which could identify subcluster characteristics of the refractory group. Bioinformatic analysis of the biological differences indicated that a number of pathologically activated signal transduction pathways are disturbed in ABVD treatment-refractory cHL.
Fatty Acid Metabolism in Myeloid-Derived Suppressor Cells and Tumor-Associated Macrophages: Key Factor in Cancer Immune Evasion
Authors: Siddiqui Sophiya , Glauben Rainer
Published: January 2022

The tumor microenvironment (TME) comprises various cell types, soluble factors, viz, metabolites or cytokines, which together play in promoting tumor metastasis. Tumor infiltrating immune cells play an important role against cancer, and metabolic switching in immune cells has been shown to affect activation, differentiation, and polarization from tumor suppressive into immune suppressive phenotypes. Macrophages represent one of the major immune infiltrates into TME. Blood monocyte-derived macrophages and myeloid derived suppressor cells (MDSCs) infiltrating into the TME potentiate hostile tumor progression by polarizing into immunosuppressive tumor-associated macrophages (TAMs). Recent studies in the field of immunometabolism focus on metabolic reprogramming at the TME in polarizing tumor-associated macrophages (TAMs). Lipid droplets (LD), detected in almost every eukaryotic cell type, represent the major source for intra-cellular fatty acids. Previously, LDs were mainly described as storage sites for fatty acids. However, LDs are now recognized to play an integral role in cellular signaling and consequently in inflammation and metabolism-mediated phenotypical changes in immune cells. In recent years, the role of LD dependent metabolism in macrophage functionality and phenotype has been being investigated. In this review article, we discuss fatty acids stored in LDs, their role in modulating metabolism of tumor-infiltrating immune cells and, therefore, in shaping the cancer progression.
Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression
Authors: Drury James , Rychahou Piotr G. , Kelson Courtney O. , Geisen Mariah E. , Wu Yuanyuan , He Daheng , Wang Chi , Lee Eun Y. , Evers B. Mark , Zaytseva Yekaterina Y.
Published: January 2022

Altered fatty acid metabolism continues to be an attractive target for therapeutic intervention in cancer. We previously found that colorectal cancer (CRC) cells with a higher metastatic potential express a higher level of fatty acid translocase (CD36). However, the role of CD36 in CRC metastasis has not been studied. Here, we demonstrate that high expression of CD36 promotes invasion of CRC cells. Consistently, CD36 promoted lung metastasis in the tail vein model and GI metastasis in the cecum injection model. RNA-Seq analysis of CRC cells with altered expression of CD36 revealed an association between high expression of CD36 and upregulation of MMP28, a novel member of the metallopeptidase family of proteins. Using shRNA-mediated knockdown and overexpression of CD36, we confirmed that CD36 regulates MMP28 expression in CRC cells. siRNA-mediated knockdown of MMP28 decreases invasion of CRC cells, suggesting that MMP28 regulates the metastatic properties of cells downstream of CD36. Importantly, high expression of MMP28 leads to a significant decrease in active E-cadherin and an increase in the products of E-cadherin cleavage, CTF1 and CTF2. In summary, upregulation of CD36 expression promotes the metastatic properties of CRC via upregulation of MMP28 and an increase in E-cadherin cleavage, suggesting that targeting the CD36–MMP28 axis may be an effective therapeutic strategy for CRC metastasis.
RhoGDI2-Mediated Rac1 Recruitment to Filamin A Enhances Rac1 Activity and Promotes Invasive Abilities of Gastric Cancer Cells
Authors: Kim Hyo-Jin , Ryu Ki-Jun , Kim Minju , Kim Taeyoung , Kim Seon-Hee , Han Hyeontak , Kim Hyemin , Hong Keun-Seok , Song Chae Yeong , Choi Yeonga , Hwangbo Cheol , Kim Kwang Dong , Yoo Jiyun
Published: January 2022

Rho GDP dissociation inhibitor 2 (RhoGDI2), a regulator of Rho family GTPase, has been known to promote tumor growth and malignant progression in gastric cancer. We previously showed that RhoGDI2 positively regulates Rac1 activity and Rac1 activation is critical for RhoGDI2-induced gastric cancer cell invasion. In this study, to identify the precise molecular mechanism by which RhoGDI2 activates Rac1 activity, we performed two-hybrid screenings using yeast and found that RhoGDI2 plays an important role in the interaction between Rac1, Filamin A and Rac1 activation in gastric cancer cells. Moreover, we found that Filamin A is required for Rac1 activation and the invasive ability of gastric cancer cells. Depletion of Filamin A expression markedly reduced Rac1 activity in RhoGDI2-expressing gastric cancer cells. The migration and invasion ability of RhoGDI2-expressing gastric cancer cells also substantially decreased when Filamin A expression was depleted. Furthermore, we found that Trio, a Rac1-specific guanine nucleotide exchange factor (GEF), is critical for Rac1 activation and the invasive ability of gastric cancer cells. Therefore, we conclude that RhoGDI2 increases Rac1 activity by recruiting Rac1 to Filamin A and enhancing the interaction between Rac1 and Trio, which is critical for the invasive ability of gastric cancer cells.
Comparative Gene Expression Analysis Reveals Similarities and Differences of Chronic Myeloid Leukemia Phases
Authors: Schwarz Annemarie , Roeder Ingo , Seifert Michael
Published: January 2022

Chronic myeloid leukemia (CML) is a slowly progressing blood cancer that primarily affects elderly people. Without successful treatment, CML progressively develops from the chronic phase through the accelerated phase to the blast crisis, and ultimately to death. Nowadays, the availability of targeted tyrosine kinase inhibitor (TKI) therapies has led to long-term disease control for the vast majority of patients. Nevertheless, there are still patients that do not respond well enough to TKI therapies and available targeted therapies are also less efficient for patients in accelerated phase or blast crises. Thus, a more detailed characterization of molecular alterations that distinguish the different CML phases is still very important. We performed an in-depth bioinformatics analysis of publicly available gene expression profiles of the three CML phases. Pairwise comparisons revealed many differentially expressed genes that formed a characteristic gene expression signature, which clearly distinguished the three CML phases. Signaling pathway expression patterns were very similar between the three phases but differed strongly in the number of affected genes, which increased with the phase. Still, significant alterations of MAPK, VEGF, PI3K-Akt, adherens junction and cytokine receptor interaction signaling distinguished specific phases. Our study also suggests that one can consider the phase-wise CML development as a three rather than a two-step process. This is in accordance with the phase-specific expression behavior of 24 potential major regulators that we predicted by a network-based approach. Several of these genes are known to be involved in the accumulation of additional mutations, alterations of immune responses, deregulation of signaling pathways or may have an impact on treatment response and survival. Importantly, some of these genes have already been reported in relation to CML (e.g., AURKB, AZU1, HLA-B, HLA-DMB, PF4) and others have been found to play important roles in different leukemias (e.g., CDCA3, RPL18A, PRG3, TLX3). In addition, increased expression of BCL2 in the accelerated and blast phase indicates that venetoclax could be a potential treatment option. Moreover, a characteristic signaling pathway signature with increased expression of cytokine and ECM receptor interaction pathway genes distinguished imatinib-resistant patients from each individual CML phase. Overall, our comparative analysis contributes to an in-depth molecular characterization of similarities and differences of the CML phases and provides hints for the identification of patients that may not profit from an imatinib therapy, which could support the development of additional treatment strategies.
Lysyl-Oxidase Dependent Extracellular Matrix Stiffness in Hodgkin Lymphomas: Mechanical and Topographical Evidence
Authors: Alfano Massimo , Locatelli Irene , D’Arrigo Cristina , Mora Marco , Vozzi Giovanni , De Acutis Aurora , Pece Roberta , Tavella Sara , Costa Delfina , Poggi Alessandro , Zocchi Maria Raffaella
Published: January 2022

Purpose: The biochemical composition and architecture of the extracellular matrix (ECM) is known to condition development and invasiveness of neoplasms. To clarify this point, we analyzed ECM stiffness, collagen cross-linking and anisotropy in lymph nodes (LN) of Hodgkin lymphomas (HL), follicular lymphomas (FL) and diffuse large B-cell lymphomas (DLBCL), compared with non-neoplastic LN (LDN). Methods and Results: We found increased elastic (Young’s) modulus in HL and advanced FL (grade 3A) over LDN, FL grade 1–2 and DLBCL. Digital imaging evidenced larger stromal areas in HL, where increased collagen cross-linking was found; in turn, architectural modifications were documented in FL3A by scanning electron microscopy and enhanced anisotropy by polarized light microscopy. Interestingly, HL expressed high levels of lysyl oxidase (LOX), an enzyme responsible for collagen cross-linking. Using gelatin scaffolds fabricated with a low elastic modulus, comparable to that of non-neoplastic tissues, we demonstrated that HL LN-derived mesenchymal stromal cells and HL cells increased the Young’s modulus of the extracellular microenvironment through the expression of LOX. Indeed, LOX inhibition by β-aminopropionitrile prevented the gelatin stiffness increase. Conclusions: These data indicate that different mechanical, topographical and/or architectural modifications of ECM are detectable in human lymphomas and are related to their histotype and grading.
Catalysis in Zeolites and Zeotypes—Cornerstone of Chemical Industry and Permanent Subject of Research
Authors: Bulánek Roman
Published: January 2022

Mankind has been aware of zeolites since 1756, when the Swede, Axel Fredrik Cronstedt (1722–1765), observed the strange behavior of a certain natural mineral (probably stellerite with some amounts of stilbite) [...]
LaNiO3 Perovskite Synthesis through the EDTA–Citrate Complexing Method and Its Application to CO Oxidation
Authors: González-Varela Daniela , Araiza Daniel G. , Díaz Gabriela , Pfeiffer Heriberto
Published: January 2022

A series of LaNiO3 materials were synthesized by the EDTA–citrate complexing method, modifying different physicochemical conditions. The LaNiO3 samples were calcined between 600 and 800 °C and characterized by XRD, SEM, XPS, CO-TPD, TG, DT, and N2 adsorption. The results evidence that although all the samples presented the same crystal phase, LaNiO3 as expected, some microstructural and superficial features varied as a function of the calcination temperature. Then, LaNiO3 samples were tested as catalysts of the CO oxidation process, a reaction never thoroughly analyzed employing this material. The catalytic results showed that LaNiO3 samples calcined at temperatures of 600 and 700 °C reached complete CO conversions at ~240 °C, while the sample thermally treated at 800 °C only achieved a 100% of CO conversion at temperatures higher than 300 °C. DRIFTS and XRD were used for studying the reaction mechanism and the catalysts’ structural stability, respectively. Finally, the obtained results were compared with different Ni-containing materials used in the same catalytic process, establishing that LaNiO3 has adequate properties for the CO oxidation process.
A Nickel Coated Copper Substrate as a Hydrogen Evolution Catalyst
Authors: Kuppam Poshan Kumar Reddy , Kimbulapitiya K. M. M. D. K. , Vuppala Srikanth , Wang Kuangye , Reddy G. Phaneendra , Pande Krishna P. , Lee Po-Tsung , Chueh Yun-Lun
Published: January 2022

Replacing precious metals with low-cost metals is the best solution for large scale production. Copper is known for its excellent conductivity and thermal management applications. When it comes to hydrogen evolution reaction, it is highly unstable, especially in KOH solution. In this paper, we approached a simple method to reduce corrosion and improve the performance by depositing nickel-molybdenum oxide and nickel on copper substrates and the achieved tafel slopes of 115 mV/dec and 117 mV/dec at 10 mA/cm2. While at first, molybdenum oxide coated samples showed better performance after 100 cycles of stability tests, the onset potential rapidly changed. Cu-Ni, which was deposited using the electron gun evaporation (e-gun), has shown better performance with 0.28 V at 10 mA/cm2 and led to stability after 100 cycles. Our results show that when copper is alloyed with nickel, it acts as a promising hydrogen evolution reaction (HER) catalyst.
Highly Efficient and Ambient-Temperature Synthesis of Benzimidazoles via Co(III)/Co(II)-Mediated Redox Catalysis
Authors: Zhong Renyuan , Xiong Wulin , Zhang Haoyuan , Zeng Tongtong , Gong Shanshan , Sun Qi
Published: January 2022

An efficient method for ambient-temperature synthesis of a variety of 2-substituted and 1,2-disubstituted benzimidazoles from aldehyde and phenylenediamine substrates has been developed by utilizing Co(III)/Co(II)-mediated redox catalysis. The combination of only 1 mol% of Co(acac)2 and stoichiometric amount of hydrogen peroxide provides a fast, green, and mild access to a diversity of benzimidazoles under solvent-free conditions.
Characterization of Oxygen Levels in an Uninfected and Infected Human Blood-Cerebrospinal-Fluid-Barrier Model
Authors: Martens Alexander , de Buhr Nicole , Ishikawa Hiroshi , Schroten Horst , von Köckritz-Blickwede Maren
Published: January 2022

The host–pathogen interaction during meningitis can be investigated with blood-cerebrospinal-fluid-barrier (BCSFB) cell culture models. They are commonly handled under atmospheric oxygen conditions (19–21% O2), although the physiological oxygen conditions are significantly lower in cerebrospinal fluid (CSF) (7–8% O2). We aimed to characterize oxygen levels in a Streptococcus (S.) suis-infected BCSFB model with transmigrating neutrophils. A BCSFB model with human choroid plexus epithelial cells growing on transwell-filters was used. The upper “blood”-compartment was infected and blood-derived neutrophils were added. S. suis and neutrophils transmigrated through the BCSFB into the “CSF”-compartment. Here, oxygen and pH values were determined with the non-invasive SensorDish® reader. Slight orbital shaking improved the luminescence-based measurement technique for detecting free oxygen. In the non-infected BCSFB model, an oxygen value of 7% O2 was determined. However, with S. suis and transmigrating neutrophils, the oxygen value significantly decreased to 2% O2. The pH level decreased slightly in all groups. In conclusion, we characterized oxygen levels in the BCSFB model and demonstrated the oxygen consumption by cells and bacteria. Oxygen values in the non-infected BCSFB model are comparable to in vivo values determined in pigs in the CSF. Infection and transmigrating neutrophils decrease the oxygen value to lower values.
The Negative Regulative Roles of BdPGRPs in the Imd Signaling Pathway of Bactrocera dorsalis
Authors: Zhang Ping , Yao Zhichao , Bai Shuai , Zhang Hongyu
Published: January 2022

Peptidoglycan recognition proteins (PGRPs) are key regulators in insects’ immune response, functioning as sensors to detect invading pathogens and as scavengers of peptidoglycan (PGN) to reduce immune overreaction. However, the exact function of PGRPs in Bactrocera dorsalis is still unclear. In this study, we identified and functionally characterized the genes BdPGRP-LB, BdPGRP-SB1 and BdPGRP-SC2 in B. dorsalis. The results showed that BdPGRP-LB, BdPGRP-SB1 and BdPGRP-SC2 all have an amidase-2 domain, which has been shown to have N-Acetylmuramoyl-l-Alanine amidase activity. The transcriptional levels of BdPGRP-LB and BdPGRP-SC2 were both high in adult stages and midgut tissues; BdPGRP-SB1 was found most abundantly expressed in the 2nd instar larvae stage and adult fat body. The expression of BdPGRP-LB and BdPGRP-SB1 and AMPs were significantly up-regulated after injury infected with Escherichia coli at different time points; however, the expression of BdPGRP-SC2 was reduced at 9 h, 24 h and 48 h following inoculation with E. coli. By injection of dsRNA, BdPGRP-LB, BdPGRP-SB1 and BdPGRP-SC2 were knocked down by RNA-interference. Silencing of BdPGRP-LB, BdPGRP-SB1 and BdPGRP-SC2 separately in flies resulted in over-activation of the Imd signaling pathway after bacterial challenge. The survival rate of the ds-PGRPs group was significantly reduced compared with the ds-egfp group after bacterial infection. Taken together, our results demonstrated that three catalytic PGRPs family genes, BdPGRP-LB, BdPGRP-SB1 and BdPGRP-SC2, are important negative regulators of the Imd pathway in B. dorsalis.
The Impact of Exercise on Telomere Length, DNA Methylation and Metabolic Footprints
Authors: Haupt Sandra , Niedrist Tobias , Sourij Harald , Schwarzinger Stephan , Moser Othmar
Published: January 2022

Aging as a major risk factor influences the probability of developing cancer, cardiovascular disease and diabetes, amongst others. The underlying mechanisms of disease are still not fully understood, but research suggests that delaying the aging process could ameliorate these pathologies. A key biological process in aging is cellular senescence which is associated with several stressors such as telomere shortening or enhanced DNA methylation. Telomere length as well as DNA methylation levels can be used as biological age predictors which are able to detect excessive acceleration or deceleration of aging. Analytical methods examining aging are often not suitable, expensive, time-consuming or require a high level of technical expertise. Therefore, research focusses on combining analytical methods which have the potential to simultaneously analyse epigenetic, genomic as well as metabolic changes.
Extracellular Vesicles Derived from Bone Marrow in an Early Stage of Ionizing Radiation Damage Are Able to Induce Bystander Responses in the Bone Marrow
Authors: Kis Dávid , Csordás Ilona Barbara , Persa Eszter , Jezsó Bálint , Hargitai Rita , Szatmári Tünde , Sándor Nikolett , Kis Enikő , Balázs Katalin , Sáfrány Géza , Lumniczky Katalin
Published: January 2022

Ionizing radiation (IR)-induced bystander effects contribute to biological responses to radiation, and extracellular vesicles (EVs) play important roles in mediating these effects. In this study we investigated the role of bone marrow (BM)-derived EVs in the bystander transfer of radiation damage. Mice were irradiated with 0.1Gy, 0.25Gy and 2Gy, EVs were extracted from the BM supernatant 24 h or 3 months after irradiation and injected into bystander mice. Acute effects on directly irradiated or EV-treated mice were investigated after 4 and 24 h, while late effects were investigated 3 months after treatment. The acute effects of EVs on the hematopoietic stem and progenitor cell pools were similar to direct irradiation effects and persisted for up to 3 months, with the hematopoietic stem cells showing the strongest bystander responses. EVs isolated 3 months after irradiation elicited no bystander responses. The level of seven microRNAs (miR-33a-3p, miR-140-3p, miR-152-3p, miR-199a-5p, miR-200c-5p, miR-375-3p and miR-669o-5p) was altered in the EVs isolated 24 hour but not 3 months after irradiation. They regulated pathways highly relevant for the cellular response to IR, indicating their role in EV-mediated bystander responses. In conclusion, we showed that only EVs from an early stage of radiation damage could transmit IR-induced bystander effects.
Aptamer-Enabled Nanomaterials for Therapeutics, Drug Targeting and Imaging
Authors: Liu Mengping , Wang Lin , Lo Young , Shiu Simon Chi-Chin , Kinghorn Andrew B. , Tanner Julian A.
Published: January 2022

A wide variety of nanomaterials have emerged in recent years with advantageous properties for a plethora of therapeutic and diagnostic applications. Such applications include drug delivery, imaging, anti-cancer therapy and radiotherapy. There is a critical need for further components which can facilitate therapeutic targeting, augment their physicochemical properties, or broaden their theranostic applications. Aptamers are single-stranded nucleic acids which have been selected or evolved to bind specifically to molecules, surfaces, or cells. Aptamers can also act as direct biologic therapeutics, or in imaging and diagnostics. There is a rich field of discovery at the interdisciplinary interface between nanomaterials and aptamer science that has significant potential across biomedicine. Herein, we review recent progress in aptamer-enabled materials and discuss pending challenges for their future biomedical application.
Dermatan Sulfate Affects Breast Cancer Cell Function via the Induction of Necroptosis
Authors: Wisowski Grzegorz , Pudełko Adam , Olczyk Krystyna , Paul-Samojedny Monika , Koźma Ewa M.
Published: January 2022

Dermatan sulfate (DS) is widespread in the extracellular matrix (ECM) of animal tissues. This glycosaminoglycan is characterized by a variable structure, which is reflected in the heterogeneity of its sulfation pattern. The sulfate groups are responsible for the binding properties of DS, which determine an interaction profile of this glycan. However, the detailed role of DS in biological processes such as the neoplasm is still poorly understood. The aim of the study was to assess the effects of the structural variants of DS on breast cancer cells. We found that DS isoforms from normal and fibrotic fascia as well as from intestinal mucosa were able to quickly induce oxidative stress in the cytoplasm and affect the mitochondrial function in luminal breast cancer cells. Moreover, the variants caused the necroptosis of the cells most likely via the first of these mechanisms. This death was responsible for a reduction in the viability and number of breast cancer cells. However, the dynamics and intensity of all of the DS variants-triggered effects were strongly dependent on the cell type and the structure of these molecules. The most pronounced activity was demonstrated by those variants that shared structural features with the DS from the tumor niche.
Hypoxia-Inducible Factor Signaling in Inflammatory Lung Injury and Repair
Authors: Evans Colin E.
Published: January 2022

Inflammatory lung injury is characterized by lung endothelial cell (LEC) death, alveolar epithelial cell (AEC) death, LEC–LEC junction weakening, and leukocyte infiltration, which together disrupt nutrient and oxygen transport. Subsequently, lung vascular repair is characterized by LEC and AEC regeneration and LEC–LEC junction re-annealing, which restores nutrient and oxygen delivery to the injured tissue. Pulmonary hypoxia is a characteristic feature of several inflammatory lung conditions, including acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and severe coronavirus disease 2019 (COVID-19). The vascular response to hypoxia is controlled primarily by the hypoxia-inducible transcription factors (HIFs) 1 and 2. These transcription factors control the expression of a wide variety of target genes, which in turn mediate key pathophysiological processes including cell survival, differentiation, migration, and proliferation. HIF signaling in pulmonary cell types such as LECs and AECs, as well as infiltrating leukocytes, tightly regulates inflammatory lung injury and repair, in a manner that is dependent upon HIF isoform, cell type, and injury stimulus. The aim of this review is to describe the HIF-dependent regulation of inflammatory lung injury and vascular repair. The review will also discuss potential areas for future study and highlight putative targets for inflammatory lung conditions such as ALI/ARDS and severe COVID-19. In the development of HIF-targeted therapies to reduce inflammatory lung injury and/or enhance pulmonary vascular repair, it will be vital to consider HIF isoform- and cell-specificity, off-target side-effects, and the timing and delivery strategy of the therapeutic intervention.
Evaluation of the Heat Produced by the Hydrothermal Liquefaction of Wet Food Processing Residues and Model Compounds
Authors: Briand Morgane , Haarlemmer Geert , Roubaud Anne , Fongarland Pascal
Published: January 2022

Hydrothermal liquefaction has proven itself as a promising pathway to the valorisation of low-value wet food residues. The chemistry is complex and many questions remain about the underlying mechanism of the transformation. Little is known about the heat of reaction, or even the thermal effects, of the hydrothermal liquefaction of real biomass and its constituents. This paper explores different methods to evaluate the heat released during the liquefaction of blackcurrant pomace and brewers’ spent grains. Some model compounds have also been evaluated, such as lignin, cellulose and glutamic acid. Exothermic behaviour was observed for blackcurrant pomace and brewers’ spent grains. Results obtained in a continuous reactor are similar to those obtained in a batch reactor. The heat release has been estimated between 1 MJ/kg and 3 MJ/kg for blackcurrant pomace and brewers’ spent grains, respectively. Liquefaction of cellulose and glucose also exhibit exothermic behaviour, while the transformation of lignin and glutamic acid present a slightly endothermic behaviour.
Development of Integrated Electrocoagulation-Sedimentation (IECS) in Continuous Mode for Turbidity and Color Removal
Authors: Bun Saret , Hong Penghour , Chawaloesphosiya Nattawin , Pang Sreynich , Vet Sreyla , Ham Phaly , Chan Rathborey , Painmanakul Pisut
Published: January 2022

The present work focused on the development and evaluation of a compact electrocoagulation (EC) reactor, combined between EC and clarifier processes in continuous modes for decolorization and turbidity removal, named the integrated electrocoagulation-sedimentation reactor (IECS). The experiments were firstly conducted in the four-liter batch column in order to optimize the EC configuration and operation condition. The removal kinetics were also investigated and predicted for kinetic correlations. After various optimization steps, the IECS reactor was conducted, consisting of EC and clarifier compartments. Liquid flow pattern in EC compartment was examined through resident time distribution technique for defining the number of EC units and divided baffles. In summary, four units of EC were placed in the EC compartment of the IECS reactor with 90% in the width of three baffles. Each EC unit had two pairs of aluminum electrode plats in monopolar arrangement with a 1.5 cm gap and required a current density of 13.5 mA/cm2. For the clarifier compartment, it was mainly designed based on the batch settling test for separating the precipitated particles. The treatment performance of the IECS reactor was tested at different liquid flows in order to reduce the pollutant to a certain level. For the individual condition, liquid flow rates of 3 and 1 L/min were defined for turbidity and color, respectively. If both pollutants are presented simultaneously, a liquid flow rate of 1–2 L/min can be used for decreasing turbidity from 250 to <20 NTU and color from 6000 to <300 ADMI.
Experimental Study on Absorption Behavior and Efficiency of Brine in Hazardous Gas Absorption Treatment
Authors: Jung Ga-young , Lee Seul-gi , Lee Jun-seo , Ma Byung-chol
Published: January 2022

There have been studies recently on bubble-column scrubbers with low cost and high efficiency for the absorption and treatment of hazardous gases in the event of a chemical spill. Bubble columns are vulnerable to freezing at temperatures below zero because the absorbents generally do not circulate. To address this issue, this study focused on the applicability, absorbed amount, and performance of brine as an absorbent. Under three different temperatures, i.e., −5 °C, −8 °C and −10 °C we examined brine (NaCl, CaCl2, and MgCl2) by varying the concentration required at each temperature. Following the experiments, CaCl2 brine was determined as the optimal brine for its absorption performance and affordability. Based on the experimental results, the absorption performance for ammonia, ethylene oxide, and methylamine, which are hazardous and water-soluble gases among accident preparedness substances (APS), was tested by using ASEPN PLUS. Our results suggested although the efficiency dropped by about 5% to 25% when brine was used as an absorbent, it can be used at the low temperatures because the gas solubility increased with decreasing temperature. Therefore, if brine, as an alternative, is used at temperatures about 15 °C, it can operate efficiently and stably without deterioration in the absorption performance. Given our experimental results and design data on the absorbed amount and absorbent replacement period for major hazardous gases are utilized to prevent bubble columns from freezing, it can be commercially used for small and medium-sized enterprises because it can help reduce installation and operation costs.
The Emotional Response to Pandemic of Middle- and High-School Students of an Italian Northern Province: The ERP Study
Authors: Povero Massimiliano , Turco Paola , Dal Negro Roberto Walter
Published: January 2022

The COVID-19 outbreak variably affected people’s mental reactions worldwide but was only episodically investigated in healthy Italian teenagers. Our aim was to investigate the emotional responses of Italian middle and high school students to the pandemic. An anonymous 10-item questionnaire was distributed in pre-selected school samples. Responders had to score their perceived extent for each reaction from 0 (lowest perception) to 10 (highest perception). A group of adults was selected as control. Generalized linear models were used to estimate differences among adults and students, high school (HS) and middle school (MS) students, and urban (U) and rural (R) MS students. Comparisons were presented as mean difference (Δ) with a 95% confidence interval (CI). A total of 1512 questionnaires (635 adults, 744 HS, 67 UMS, and 66 RMS) were analyzed. Students appeared more indifferent (Δ = 1.97, 1.52–2.41), anxious (Δ = 0.56, 0.07–1.04), aggressive (Δ = 2.21, 1.72–2.70), and depressed (Δ = 1.87, 1.40–2.34) than adults did, and claimed a higher loss of interest in their activities (Δ = 1.21, 0.72–1.70). Students were less disbelieving (Δ = −0.93, −1.50–0.35) and feared for their loved ones (Δ = −0.89, −1.40–0.39). MS students were less affected by the outbreak than HS students were. Furthermore, R-MS students were significantly less aggressive and depressed, but more indifferent and disbelieving than U-MS. Female sex was an independent factor associated to almost all the questionnaire domains. The pattern of the psychological responses to the pandemic in Italian students proved multifaceted. In addition to anxiety, loss of interest in activities, and depression, aggressiveness emerged as the most characterizing mental attitude in response to the pandemic.
Ear, Nose, and Throat Foreign Bodies in Children: A Retrospective Study
Authors: Kwon Bin , Choi Yeso , Kim Sung-Kyun , Hong Seok-Jin , Kim Yong-Bok , Hong Seok-Min
Published: January 2022

Background: This study analyzed the presentation, characteristics, and management of foreign bodies in different age groups of pediatric patients with ear, nose, and throat foreign bodies. Methods: A retrospective study was performed using data from October 2012 to September 2020. A total of 1285 patients with ear, nose, and throat foreign bodies who were less than 12 years of age and who presented to the emergency room were included in this study. Their biographical data, clinical presentations, foreign body types and locations, and management outcomes were obtained from medical records and analyzed as three age groups (infancy: <2 years old; early childhood: 2–5 years old; and late childhood: 6–12 years old). Results: The early childhood group had the highest number of patients (n = 672; 52.2%). Throat was the most common location (59.2%), and bone was the most common type of foreign body. Among the children who visited our hospital, foreign bodies were actually found in only 657 patients (51.1%) and removed by an otolaryngologist in 625 (95.1%) cases. Conclusion: Our study could provide guidance for the diagnosis and management of pediatric patients who present to emergency departments with foreign bodies.
Vascular Access in Pediatric Oncology and Hematology: State of the Art
Authors: Crocoli Alessandro , Martucci Cristina , Persano Giorgio , De Pasquale Maria Debora , Serra Annalisa , Accinni Antonella , Aloi Ivan Pietro , Bertocchini Arianna , Frediani Simone , Madafferi Silvia , Pardi Valerio , Inserra Alessandro
Published: January 2022

Management and successful use of vascular access are critical issues in pediatric patients affected by malignancies. Prolonged course of disease, complex and various treatment protocols require long-lasting vascular access providing adequate tools to administrate those therapies and to collect routine blood sampling without painful and repeated venipuncture. For these reasons, central venous catheters are currently an important component in pediatric onco-hematological care, with a direct influence on outcome. Indeed, there are peculiar issues (techniques of insertion, management, complications etc.) which must be well-known in order to improve the outcome and the quality of life of children with cancer.