bluuebunny/crossref_metadata_2025 huggingface dataset:
Death by Frostbite: From Theory to Reality: A Case Report
Authors: Duma Stefano , Mele Federica , Calvano Mariagrazia , Leonardelli Mirko , Macorano Enrica , De Gabriele Giovanni , Cristalli Alessandro , Marzullo Andrea , Introna Francesco
Published: October 2024

Background: When the body temperature falls below 35 °C, a condition known as hypothermia occurs. This renders it impossible to regulate the body temperature and produce heat. In these cases, death is due to a cessation of vital functions and is triggered by a body temperature below 25 °C. A multidisciplinary approach is typically required when hypothermia is suspected to combine circumstantial evidence, external examination, autopsy, microscopic and biochemical findings, and other data useful to assess the diagnosis of fatal hypothermia. Post-mortem diagnosis of death by hypothermia frequently presents a medico-legal dilemma. Methods and Results: The authors present a case of hypothermia in which site inspection, autopsy, and histological examination simultaneously revealed the forensic and anatomopathological characteristic findings of hypothermia with some peculiar evidence: paradoxical undressing, thanatochronological phenomena, frost erythema, polyvisceral congestion and Wischnewski spots, and subnuclear vacuolization of renal tubular epithelial cells.
Simulation of the Stackelberg–Hotelling Game
Authors: Garcia-Perez Luis , Grau-Climent Juan , Losada Juan C. , Alonso-Sanz Ramon
Published: October 2024

This work studies the Hotelling game with sequential choice of prices, that is, the Stackelberg–Hotelling (SHOT) game. The game is studied through numerical simulation, which provides the subgame perfect equilibrium solution not only in the unrestricted game but also in the game with reservation cost and with elastic demand. The simulation technique is tested first in the unconstrained game, where the analytical subgame perfect equilibrium solution was already known. Then, the numerical procedure is generalized to cope with the SHOT game with reservation cost and with elastic demand. These enriched formulations of the SHOT game have not been studied so far, so this article provides an exploratory study of them.
Biocompatible Hydrogel Coating on Silicone Rubber with Improved Antifouling and Durable Lubricious Properties
Authors: Gao Shuai , Liu Zheng , Zeng Wei , Zhang Yunfeng , Zhang Fanjun , Wu Dimeng , Wang Yunbing
Published: October 2024

Silicone rubber is widely used in various medical applications. However, silicone rubber is prone to biofouling due to their affinity for lipids and has a high friction coefficient, which can significantly impact their efficacy and performance used as medical devices. Thus, the development of hydrogels with antifouling and lubricious abilities for the modification of silicone rubber is in high demand. We herein prepared a variety of hydrogel coatings mainly based on polyvinylpyrrolidone (PVP) and poly (ethylene glycol) diacrylate (PEGDA). We modified the silicone rubber using the prepared hydrogel coatings and cured it using a heating method. Then, we characterized its surface and evaluated the antifouling property, lubricious property, cytotoxicity, sensitization, and vaginal irritation. The results of water contact angle (WCA), protein adsorption, and friction coefficient indicated the success of the modification of the silicone rubber, leading to a significant decrease in the corresponding test values. Meanwhile, the results of cytotoxicity, sensitization, and vaginal irritation tests showed that the hydrogel coating-modified silicone rubbers have an excellent biocompatibility. This study describes how the silicone rubber could be modified with a biocompatible hydrogel coating. The hydrogel coating-modified silicone rubbers have improved antifouling and durable lubricious properties.
Structural Build-Up and Stability of Hybrid Monoglyceride–Triglyceride Oleogels
Authors: Rondou Kato , Dewettinck Antonia , Dewettinck Koen , Van Bockstaele Filip
Published: October 2024

Oleogelation is an alternative oil structuring route to formulate (semi-)solid fats with a reduced amount of saturated fats. Monoglycerides have been identified as effective gelators; however, their application potential can be limited due to challenges regarding mechanical strength and long-term stability. Therefore, the formulation of hybrid fat blends is a promising way to improve the functionality of oleogels. This research focuses on the interaction between mono- and triglycerides (MAGs and TAGs) in hybrid oleogels. A total gelator concentration of 10% (w/w) with changing MAGs–TAGs ratios (increase by 25% on a molar basis; M0-T100, M25-T75, M50-T50, M75-T25, M100-T0) was used. First, the oleogels were produced without shear to unravel the crystallization behavior (DSC, SAXS, WAXS). Next, the oleogels were crystallized with shear to assess the interactions between MAGs and TAGs on macroscale properties (rigidity, oil binding capacity) during storage of 1 day, 1 week, and 4 weeks. A clear distinction could be made between the MAG crystals and TAG crystals in the blends M50-T50 and M75-T25 based on WAXS, SAXS, and phase contrast microscopy. This indicates that both gelators crystallize separately. During the follow-up study of the dynamically produced samples, a synergistic effect was found for Dy-M50-T50 and Dy-M75-T25; however, it was not maintained upon storage. The initial rigidity of 2.4 × 104 Pa and 2.0 × 104 Pa decreased to 1.5 × 104 Pa and 1.0 × 104 Pa for Dy-M50-T50 and Dy-M75-T25, respectively.
Long-Term Investigation of Nano-Silica Gel for Water Shut-Off in Fractured Reservoirs
Authors: Ali Ahmed , Al Ramadan Mustafa , Aljawad Murtada Saleh
Published: October 2024

Silicate gels have long been utilized as water shut-off agents in petroleum fields to address excessive water production. In recent years, nano-silica gel has emerged as a promising alternative to traditional silicate gels, offering potentially improved plugging performance. However, the long-term effectiveness of these gels remains uncertain, posing challenges to sustained profitability. Therefore, a comprehensive study spanning 6 months was conducted on fractured and induced channel samples treated with nano-silica gel of 75/25 wt% (silica/activator) at 200 °F. A comparative analysis was performed with samples treated using polyacrylamide/polyethyleneimine PAM/PEI gel (9/1 wt%) to compare the performance of both systems. Throughout the aging period in formation water at 167 °F, endurance tests were conducted at regular intervals, complemented by computed tomography (CT) scans to monitor any potential degradation. The results revealed nano-silica gel’s superior long-term performance in plugging fractures and channels compared to PAM/PEI gel. Even after 6 months, the nano-silica gel maintained a remarkable 100% plugging efficiency at 1000 psi, with a maximum leak-off rate of 0.088 cc/min in the mid-fractured sample and 0.027 in the induced channel sample. In comparison, PAM/PEI gel exhibited a reduction in efficiency to 99.15% in the fractured sample (5.5 cc/min maximum leak-off rate) and 99.99% in the induced channel sample (0.036 cc/min maximum leak-off rate). These findings highlight the potential of nano-silica gel as a more durable water shut-off agent for managing water production in fractures and channels.
Effect of Foot Orthoses and Footwear in People with Rheumatoid Arthritis: An Updated Systematic Review
Authors: Cabrera-Sánchez José Manuel , Reina-Bueno María , Palomo-Toucedo Inmaculada C. , Vázquez-Bautista María del Carmen , Núñez-Baila María-Ángeles , González-López José Rafael
Published: October 2024

Background/Objectives: Rheumatoid arthritis is a chronic, systemic, inflammatory disease of an autoimmune nature that causes pain and disability in affected patients. Foot pain has become a challenge due to its negative impact on physical function. The objective of this updated systematic review is to describe the effect of foot orthoses and/or footwear in patients with rheumatoid arthritis and foot problems. Methods: Scopus, PubMed, CINALH, WOS, and Dialnet were searched for all articles published from January 2013 to September 2024. Inclusion criteria included randomised clinical trials and crossover trials (level of evidence I), published within the last 10 years, involving adults with a diagnosis of rheumatoid arthritis, with no restrictions on gender, race, or ethnicity. All studies that addressed the use of foot orthoses and/or shoe therapy in any type of comparison between these interventions were considered relevant. Review Manager was used to carry out the bias analysis of the selected studies. The reporting was based on the new PRISMA guidelines. Results: A total of 9 relevant articles were selected from an initial sample of 438. These articles analyse and compare the effectiveness of various types of foot orthoses in reducing pain, functional limitation, and disability, as well as improving balance and kinetic and kinematic parameters affected by rheumatoid arthritis. Conclusions: Foot orthoses reduce pain and disability in rheumatoid arthritis, improving balance and kinematic parameters. However, no significant improvements in the patients’ functionality and walking ability have been demonstrated. Customised ones with good arch control, heel reinforcement, and metatarsal pad are more effective. No results on the impact of footwear on patients with rheumatoid arthritis have been found in the last 10 years. This systematic review was registered in PROSPERO (CRD42023405645).
Exploring the Link between Xerostomia and Oral Health in Mental Illness: Insights from Autism Spectrum Disorder, Depression, Bipolar Disorder, and Schizophrenia
Authors: Chen Rou-Jun , Lai Kuei-Hung , Lee Chun-Hung , Lin Hao-Ying , Lin Cheng-Chieh , Chen Chi-Hsiu , Chen Wei , Chen Wei-Yu , Vo Thi Thuy Tien , Lee I-Ta
Published: October 2024

Background: The relationship between mental disorders and oral health is complex, involving behavioral, biological, and psychosocial factors. This review aims to investigate the impact of mental disorders, including autism spectrum disorder (ASD), depression, bipolar disorder, and schizophrenia, on oral health outcomes. Methods: A comprehensive review of existing literature was conducted to analyze the oral health outcomes associated with each mental disorder. The focus was on examining dietary habits, oral hygiene behaviors, physiological changes, and medication side effects that contribute to oral health issues. Results: The findings indicate that individuals with ASD often exhibit unique dietary habits and reduced oral hygiene capabilities due to sensory sensitivities, leading to a higher prevalence of dental caries and periodontal diseases. Depression and bipolar disorder are associated with physiological changes such as reduced saliva production and poor oral hygiene behaviors, increasing the risk of oral health problems. Medications used for these conditions exacerbate issues like xerostomia, further elevating the risk of dental diseases. Schizophrenia poses additional challenges, including cognitive impairments and medication side effects that hinder effective oral care, heightening susceptibility to oral diseases. Conclusions: This review highlights the specific oral health challenges associated with different mental disorders and emphasizes the need for tailored dental care strategies that integrate mental health considerations. The study contributes to the literature by demonstrating the unique oral health impacts of these disorders. However, the findings are limited by the scope of available cross-sectional data and the absence of longitudinal studies. Future research should focus on longitudinal and intervention-based studies to explore causal relationships and develop effective treatments.
Changes in Trait Mindfulness after a Brief Mindfulness Training Program of Self-Breathing
Authors: Sakagami Momoe , Yokono Tomoe , Abeywickrama Hansani Madushika , Seki Nao , Miyasaka Michio , Uchiyama Mieko
Published: October 2024

Background: Developing and cultivating mindfulness exerts a positive effect on psychological and cognitive performance. Sharpening the skill requires continuous mindfulness-based training (MT), which can be challenging for people leading busy lives. Therefore, the current study examined whether trait mindfulness can be improved by a flexible and brief MT program of self-breathing using a pre–post intervention design. Methods: Trait mindfulness was assessed using the Japanese version of the Five Facet Mindfulness Questionnaire (FFMQ) before the intervention (pre), after 2 weeks (during), and 4 weeks after the intervention started (post). Data were analyzed using the Friedman test followed by the Dunn–Bonferroni correction. Results: The study sample consisted of 22 healthy participants aged from 20 to 60 years with no previous experience with yoga or meditation equivalent to MT. The mean number of days of MT practice was 26.4, and 11 participants had interruptions. The median values of pre-, during-, and post-total FFMQ scores were 115.5, 123, and 129, respectively. Significant differences were observed in the total pre and post (p < 0.001) and during and post (p = 0.002) FFMQ scores, though a medium effect was found (r = 0.30) only between the pre and post scores. Of the five sub-scales of FFMQ, significant differences were observed only between pre and post Observing (p = 0.01), Nonreactivity (p < 0.001), and Describing (p = 0.01), and during and post Nonjudging (p = 0.016), and Nonreactivity (p = 0.025). Conclusions: Our findings suggest that the simple, brief, and flexible self-breathing method employed in this study has a substantial effect on fostering trait mindfulness and, therefore, can be adopted by people with hectic daily schedules.
Exploring the Effects of Qigong, Tai Chi, and Yoga on Fatigue, Mental Health, and Sleep Quality in Chronic Fatigue and Post-COVID Syndromes: A Systematic Review with Meta-Analysis
Authors: Fricke-Comellas Hermann , Heredia-Rizo Alberto Marcos , Casuso-Holgado María Jesús , Salas-González Jesús , Fernández-Seguín Lourdes María
Published: October 2024

Background/Objectives: Chronic fatigue syndrome (CFS) and post-COVID syndrome (PCS) pose a substantial socioeconomic burden. The aim of this systematic review was to assess current evidence regarding the effect of the most popular forms of movement-based mindful exercises, i.e., qigong, tai chi, and yoga, on fatigue and associated symptoms in CFS and PCS. Methods: CINAHL, Embase, PsycINFO, PubMed, Scopus, and the Cochrane Library were searched from inception to October 2023. Randomized controlled trials (RCTs) where qigong, tai chi, or yoga were compared with waitlist, no intervention, or active controls were included. Independent reviewers participated in data extraction, and evaluated risk of bias, spin of information, completeness of intervention description, and certainty of the evidence (GRADE). Meta-analyses were conducted. The primary outcome was the level of fatigue. Secondary measures were the severity of anxiety and depressive symptoms and sleep quality. Results were expressed as mean difference (MD) or standardized mean difference (SMD) with a 95% confidence interval (CI). Results: Thirteen RCTs with 661 participants were included, with most studies presenting a moderate or high risk of bias. Mindful exercises were more effective than control interventions to alleviate fatigue: SMD (95%CI) = −0.44 (−0.63 to −0.25), I2 = 48%, p < 0.0001. Positive effects were also observed for secondary outcomes. The certainty of the evidence was low or very low. Conclusions: Qigong, tai chi, and yoga may be effective to reduce fatigue and improve anxiety, depression, and sleep quality in adults with CFS or PCS. However, serious methodological concerns limit the clinical applicability of these findings.
Examining Physicians’ Approaches to Treating Relatives in Primary Health Care Centers: Insights from a Qualitative Study
Authors: Alhamdan Manal R. , Aloudah Nouf M. , Alrajhi Saleh
Published: October 2024

Introduction: Family medicine physicians take care of a diverse population of patients with a variety of acute and chronic diseases. These patients include family, friends, and acquaintances who may ask for direct medical care or help in accessing healthcare products and services within or outside of officially approved procedures. This is ethically challenging due to an ambiguous medical code of ethics, but it is commonly accepted as normal behavior by society. The aim of this study was to explore family medicine physicians’ perspectives regarding the benefits, difficulties, and ethics of responding to medical care requests and/or favors from family, relatives, friends, and acquaintances and to make recommendations. Methods: The study sample consisted of junior and senior family medicine physicians working in primary healthcare centers affiliated with the Ministry of Health in Saudi Arabia. In-depth semi-structured interviews were conducted to collect data. Using social exchange theory, this qualitative study explores how family medicine physicians perceive and handle requests for medical favors from family members and others. Results: Semi-structured interviews were conducted with 19 family medicine physicians (six focus groups) with clinical experience ranging from 3 to 20 years. The data analysis identified three themes: perceived benefits and costs of cultural and social connectedness, shortcomings in patient management and healthcare systems, and recommendations to address challenges between physicians and patients who are relatives. Discussion and Conclusions: This study shows that treating others outside of normal access to healthcare services presents several ethical, moral, and professional challenges. Therefore, policy adaptation requires understanding this intricate dilemma and improving laws, system regulations, and guidelines for physicians and community members to improve access to care, reduce system abuse, empower providers, and enhance community awareness and compliance.
Determining the Need for Palliative Care Patients with Multiple Sclerosis—A Cross-Sectional Study
Authors: Aranđelović Branimirka , Simić Svetlana , Simin Dragana , Mikić Milena , Dolinaj Vladimir , Bogdanović Vasić Slobodanka , Milutinović Dragana
Published: October 2024

Background/Objectives: Multiple sclerosis is characterised by the manifestation of heterogeneous symptoms that affect daily functioning. Patients face physical impairment, psychological problems and socioeconomic changes. Depending on the form of the disease, they may have different needs, which are often unsatisfied and could be overcome by including palliative care in the treatment. Despite the above, not enough is known about this population’s needs for palliative care. This cross-sectional study aimed to identify the need for palliative care and assess the functional dependence level in daily living activities in patients with multiple sclerosis. Methods: The sample consisted of 120 patients from the Neurology Clinic of the University Clinical Center of Vojvodina, Serbia. The following research instruments were used: a General questionnaire, Sheffield Profile for Assessment and Referral for Care, Multiple Sclerosis Impact Scale and Barthel Index. Results: The need for palliative care was expressed by 36.7% of patients, who expressed the most concern for the physical symptoms, independence and activity domains. Also, it was determined that the disease prevents patients from doing demanding physical tasks and that they are worried about multiple sclerosis. Almost a quarter of patients have had complete/severe dependence on other persons in performing daily activities. Conclusions: The most frequently reported physical symptoms by patients with MS were weakness, bladder problems, fatigue and different levels of dependence, while concerns related to sexual health, low mood and anxiety emerged as key challenges in the psychological domain. These findings underscore the importance of conducting needs assessments to guide the development of an appropriate palliative care model for MS patients.
Developing Self-Compassion in Healthcare Professionals Utilising a Brief Online Intervention: A Randomised Waitlist Control Trial
Authors: Super Amanda , Yarker Joanna , Lewis Rachel , Keightley Samuel , Summers Denvar , Munir Fehmidah
Published: October 2024

(1) Background: The level of stress experienced by staff in the healthcare sector is highly prevalent and well documented. Self-compassion may support the health and wellbeing of individuals and enable them to stay well at work. This study aimed to understand whether a brief, online, self-guided, novel intervention improved the health and wellbeing of healthcare professionals. (2) Methods: In a parallel randomised controlled trial, a volunteer sample of healthcare professionals were assigned to an intervention group (n = 110) or a waitlist control group (n = 80). Measures of self-compassion, mental wellbeing, stress and burnout were collected by an online questionnaire at baseline, post-programme and, for the intervention group, at follow-up. (3) Results: This intervention appeared to be effective in increasing self-compassion and mental health and decreasing stress and burnout. Significant group effects and significant time × group interactions for overall self-compassion [F (2, 183) = 32.72, p < 0.001; effect size ηp2 = 0.226], mental wellbeing [F (2, 212) = 17.46, p < 0.001; effect size ηp2 = 0.135], perceived stress [F (2, 205) = 5.42, p = 0.006; effect size ηp2 = 0.46], personal burnout [F (2, 224) = 7.57, p = 0.001; effect size ηp2 = 0.063] and work burnout [F (2, 208) = 7.39, p = 0.001; effect size ηp2 = 0.062] were found. (4) Conclusions: This study shows promise that an affordable and scalable intervention can be effective for busy healthcare professionals operating in a significantly challenging environment.
The Role of MicroRNA in the Pathophysiology and Diagnosis of Viral Myocarditis
Authors: Młynarska Ewelina , Badura Krzysztof , Kurciński Szymon , Sinkowska Julia , Jakubowska Paulina , Rysz Jacek , Franczyk Beata
Published: October 2024

Myocarditis is a non-ischemic condition with a heterogeneous etiology, clinical course and prognosis. The most common etiology of myocarditis are viral infections, whereas the most severe complications are acute and chronic heart failure and sudden cardiac death. The heterogeneous clinical course of the disease, as well as the availability and costs of diagnostic tools such as cardiac magnetic resonance and endomyocardial biopsy, hinder the diagnosis of myocarditis and its underlying cause. Non-coding RNAs such as micro-RNAs (miRNAs; miR) have been shown to be involved in the disease’s pathophysiology; however, their potential in disease diagnosis and treatment should also be considered. Non-coding RNAs are RNAs that are not translated into proteins, and they have the ability to regulate several intracellular pathways. MiRNAs regulate gene expression by binding with their targets and inhibiting protein synthesis by interfering with the translation of coding genes or causing the degradation of messenger RNA. Several miRNAs, such as miR-1, -133, -21, -15, -98, -126, -155, -148, -203, -208, -221, -222, -203 and -590, have been shown to be involved in the pathophysiology of viral myocarditis (VMC), and some of them have been shown to have diagnostic abilities. This article summarizes the available data on miRNAs and their associations with VMC.
Electron-Transferring Flavoprotein and Its Dehydrogenase Required for Fungal Pathogenicity in Arthrobotrys oligospora
Authors: Liu Yonglan , Li Zhangyu , Liu Junjie , Zhang Xiqi , Wang Xin
Published: October 2024

Electron transfer flavoprotein (ETF) plays an important function in fatty acid beta oxidation and the amino acid metabolic pathway. It can provide pathogenicity to some opportunistic fungi via modulating cellular metabolite composition. Arthrobotrys oligospora is a typical invasion fungus to nematodes. Its ETF characterization is still unknown. Here, we showed that the mutations of A. oligospora ETF (Aoetfα and Aoetfβ) and its dehydrogenase (Aoetfdh) led to severe defects in mitochondrial integrity and blocked fatty acid metabolism. The pathogenicity-associated trap structures were completely suppressed when exposed to nematode-derived ascarosides and nutrition signals, including ammonia and urea. Compared to the wild-type strain, the nematode predatory activity was significantly reduced and delayed. But surprisingly, the rich nutrition could restore the massive trap and robust predatory activity in the mutant Aoetfβ beyond all induction cues. Moreover, the deletion of Aoetfβ has led to the accumulation of butyrate-like smell, which has a strong attraction to Caenorhabditis elegans nematodes. Ultimately, ETF and its dehydrogenase play a crucial role in nematode-trapping fungi, highlighting mitochondrial metabolite fluctuations that are connected to pathogenesis and further regulating the interactions between fungi and nematodes.
Developmental and Molecular Effects of C-Type Natriuretic Peptide Supplementation in In Vitro Culture of Bovine Embryos
Authors: Costa Camila Bortoliero , Silva Nathália Covre da , Silva Amanda Nespolo , Pioltine Elisa Mariano , Dellaqua Thaisy Tino , Zangirolamo Amanda Fonseca , Meirelles Flávio Vieira , Seneda Marcelo Marcondes , Nogueira Marcelo Fábio Gouveia
Published: October 2024

The use of C-type natriuretic peptide (CNP) in the interaction with the oocyte and in the temporary postponement of spontaneous meiosis resumption has already been well described. However, its action in pre-implantation developmental-stage embryos is yet to be understood. Thus, our study aimed to detect the presence of the canonical CNP receptor (natriuretic peptide receptor, NPR2) in germinal vesicle (GV)-, metaphase II (MII)-, presumptive zygote (PZ)-, morula (MO)-, and blastocyst (BL)-stage embryos and, later, to observe possible modulations on the embryos when co-cultured with CNP. In Experiment I, we detected and quantified NPR2 on the abovementioned embryo stages. Further, in Experiment II, we intended to test different concentrations (100, 200, or 400 nM of CNP) at different times of inclusion in the in vitro culture (IVC; inclusion from the beginning, i.e., day 1, or from day 5). In Experiment III, 400 nM of CNP was used on day 1 (D1) in the IVC, which was not demonstrated to be embryotoxic, and it showed potentially promising results in the blastocyst production rate when compared to the control. Thus, we analyzed the embryonic development rates of bovine embryos (D7) and hatching kinetics (D7, D8, and D9). Subsequently, morula and blastocyst were collected and evaluated for transcript abundance of their competence and quality (apoptosis, oxidative stress, proliferation, and differentiation) and lipid metabolism. Differences with probabilities less than p < 0.05, and/or fold change (FC) > 1.5, were considered significant. We demonstrate the presence of NPR2 until the blastocyst development stage, when there was a significant decrease in membrane receptors. There was no statistical difference in the production rate after co-culture with 400 nM CNP. However, when we evaluated the abundance of morula transcripts, there was an upregulated transcription in ADCY6 (p = 0.057) and downregulated transcripts in BMP15 (p = 0.013), ACAT1 (p = 0.040), and CASP3 (p = 0.082). In addition, there was a total of 12 transcriptions in morula that presented variation FC > 1.5. In blastocysts, the treatment with CNP induced upregulation in BID, CASP3, SOX2, and HSPA5 transcripts and downregulation in BDNF, NLRP5, ELOVL1, ELOVL4, IGFBP4, and FDX1 transcripts (FC > 1.5). Thus, our study identified and quantified the presence of NPR2 in bovine pre-implantation embryos. Furthermore, 400 nM of CNP in IVC, a concentration not previously described in the literature, modulated some transcripts related to embryonic metabolism, and this was not embryotoxic morphologically.
AtC3H3, an Arabidopsis Non-TZF Gene, Enhances Salt Tolerance by Increasing the Expression of Both ABA-Dependent and -Independent Stress-Responsive Genes
Authors: Seok Hye-Yeon , Lee Sun-Young , Nguyen Linh Vu , Bayzid Md , Jang Yunseong , Moon Yong-Hwan
Published: October 2024

Salinity causes widespread crop loss and prompts plants to adapt through changes in gene expression. In this study, we aimed to investigate the function of the non-tandem CCCH zinc-finger (non-TZF) protein gene AtC3H3 in response to salt stress in Arabidopsis. AtC3H3, a gene from the non-TZF gene family known for its RNA-binding and RNase activities, was up-regulated under osmotic stress, such as high salt and drought. When overexpressed in Arabidopsis, AtC3H3 improved tolerance to salt stress, but not drought stress. The expression of well-known abscisic acid (ABA)-dependent salt stress-responsive genes, namely Responsive to Desiccation 29B (RD29B), RD22, and Responsive to ABA 18 (RAB18), and representative ABA-independent salt stress-responsive genes, namely Dehydration-Responsive Element Binding protein 2A (DREB2A) and DREB2B, was significantly higher in AtC3H3-overexpressing transgenic plants (AtC3H3 OXs) than in wild-type plants (WT) under NaCl treatment, indicating its significance in both ABA-dependent and -independent signal transduction pathways. mRNA-sequencing (mRNA-Seq) analysis using NaCl-treated WT and AtC3H3 OXs revealed no potential target mRNAs for the RNase function of AtC3H3, suggesting that the potential targets of AtC3H3 might be noncoding RNAs and not mRNAs. Through this study, we conclusively demonstrated that AtC3H3 plays a crucial role in salt stress tolerance by influencing the expression of salt stress-responsive genes. These findings offer new insights into plant stress response mechanisms and suggest potential strategies for improving crop resilience to salinity stress.
The Role of Ligand Exchange in Salen Cobalt Complexes in the Alternating Copolymerization of Propylene Oxide and Carbon Dioxide
Authors: Rzhevskiy Sergey A. , Shurupova Olga V. , Asachenko Andrey F. , Plutalova Anna V. , Chernikova Elena V. , Beletskaya Irina P.
Published: October 2024

A comparative study of the copolymerization of racemic propylene oxide (PO) with CO2 catalyzed by racemic (salcy)CoX (salcy = N,N′-bis(3,5-di-tert-butylsalicylidene)-1,2-diaminocyclohexane; X = perfluorobenzoate (OBzF5) or 2,4-dinitrophenoxy (DNP)) in the presence of a [PPN]Cl ([PPN] = bis(triphenylphosphine)iminium) cocatalyst is performed in bulk at 21 °C and a 2.5 MPa pressure of CO2. The increase in the nucleophilicity of an attacking anion results in the increase in the copolymerization rate. Racemic (salcy)CoX provides a high selectivity of the copolymerization, which can be higher than 99%, and the living polymerization mechanism. Poly(propylene carbonate) (PPC) with bimodal molecular weight distribution (MWD) is formed throughout copolymerization. Both modes are living and are characterized by low dispersity, while their contribution to MWD depends on the nature of the attacking anion. The racemic (salcy)CoDNP/[PPN]DNP system is found to be preferable for the production of PPC with a high yield and selectivity.
WRKY Transcription Factors in Response to Metal Stress in Plants: A Review
Authors: Huang Yuanzhi , Sun Zhaofei , Zhou Xiangui
Published: October 2024

Heavy metals in soil can inflict direct damage on plants growing within it, adversely affecting their growth height, root development, leaf area, and other physiological traits. To counteract the toxic impacts of heavy metals on plant growth and development, plants mitigate heavy metal stress through mechanisms such as metal chelation, vacuolar compartmentalization, regulation of transporters, and enhancement of antioxidant functions. WRKY transcription factors (TFs) play a crucial role in plant growth and development as well as in responses to both biotic and abiotic stresses; notably, heavy metal stress is classified as an abiotic stressor. An increasing number of studies have highlighted the significant role of WRKY proteins in regulating heavy metal stress across various levels. Upon the entry of heavy metal ions into plant root cells, the production of reactive oxygen species (ROS) is triggered, leading to the phosphorylation and activation of WRKY TFs through MAPK cascade signaling. Activated WRKY TFs then modulate various physiological processes by upregulating or downregulating the expression of downstream genes to confer heavy metal tolerance to plants. This review provides an overview of the research advancements regarding WRKY TFs in regulating heavy metal ion stress—including cadmium (Cd), arsenic (As), copper (Cu)—and aluminum (Al) toxicity.
Intricate Evolution of Multifunctional Lipoxygenase in Red Algae
Authors: Zhu Zhujun , Li Yanrong , Wu Xinru , Li Jia , Mo Xiaodong , Yan Xiaojun , Chen Haimin
Published: October 2024

Lipoxygenases (LOXs) from lower organisms have substrate flexibility and function versatility in fatty acid oxidation, but it is not clear how these LOXs acquired the ability to execute multiple functions within only one catalytic domain. This work studied a multifunctional LOX from red alga Pyropia haitanensis (PhLOX) which combined hydroperoxidelyase (HPL) and allene oxide synthase (AOS) activity in its active pocket. Molecular docking and site-directed mutagenesis revealed that Phe642 and Phe826 jointly regulated the double peroxidation of fatty acid, Gln777 and Asn575 were essential to the AOS function, and the HPL activity was improved when Asn575, Gln777, or Phe826 was replaced by leucine. Phylogenetic analysis indicated that Asn575 and Phe826 were unique amino acid sites in the separated clades clustered with PhLOX, whereas Phe642 and Gln777 were conserved in plant or animal LOXs. The N-terminal START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC) domain of PhLOX was another key variable, as the absence of this domain disrupted the versatility of PhLOX. Moreover, the functions of two homologous LOXs from marine bacterium Shewanella violacea and red alga Chondrus crispus were examined. The HPL activity of PhLOX appeared to be inherited from a common ancestor, and the AOS function was likely acquired through mutations in some key residues in the active pocket. Taken together, our results suggested that some LOXs from red algae attained their versatility by amalgamating functional domains of ancestral origin and unique amino acid mutations.
Robotic Rectosigmoid Resection with Totally Intracorporeal Colorectal Anastomosis (TICA) for Recurrent Ovarian Cancer: A Case Series and Description of the Technique
Authors: Gallotta Valerio , Palmieri Luca , Santullo Francesco , Certelli Camilla , Lodoli Claudio , Abatini Carlo , El Halabieh Miriam Attalla , D’Indinosante Marco , Federico Alex , Rosati Andrea , Conte Carmine , Oliva Riccardo , Fagotti Anna , Scambia Giovanni
Published: October 2024

Background: Most patients with ovarian cancer relapse within 2 years. Prospective randomized trials, such as DESKTOP III and SOC-I, have shown the role of secondary cytoreduction in improving oncological outcomes in selected patients, when complete tumor resection is achieved. Recent retrospective series suggest that minimally invasive surgery is a feasible option in oligometastatic recurrences, such as rectal ones. Methods: Five patients with an isolated rectal recurrence infiltrating the bowel wall underwent a robotic rectosigmoid resection with totally intracorporeal colorectal anastomosis. The procedure began with retroperitoneal access to manage the vascular structures, followed by visceral resection with a minimally invasive approach. The standard steps of an en-bloc pelvic resection, including intracorporeal end-to-end anastomosis, were performed. The treatment data were evaluated. Results: The mean age of the patients was 54 years, and their mean body mass index was 30. All patients had at least one previous abdominal surgery and 60% had high-grade serous ovarian cancer at their initial diagnosis. Their mean platinum-free interval was 17.4 months. Complete secondary cytoreduction was achieved in all cases, with histopathology confirming bowel infiltration. The mean procedure duration was 294 min, with an estimated blood loss of 180 mL. No intraoperative complications occurred. The mean hospital stay was 8 days. One patient had a grade 2 postoperative complication. The mean follow-up period was 14 months, with only one patient experiencing a recurrence at the level of the abdominal wall. Conclusions: Robotic rectosigmoid resection is a viable option for complete cytoreduction in isolated recurrent ovarian cancer.
Assessing the Imbalances in Growth between Urban Land and Urban Population and the Influencing Factors: An Allometric Growth Perspective
Authors: Qiao Wenyi , Yin Shanggang , Huang Xianjin
Published: October 2024

Maintaining a balance between urban land (UL) expansion and urban population (UP) growth is one of the goals of sustainable development, and maintaining this balance requires more theoretical exploration and regional experience. This paper re-evaluated the imbalances in growth between urban land and urban population (IGULUP) from the perspective of allometric growth and explored its influencing mechanism, taking urban agglomerations (UAs) in China as a case. This paper reveals that the growth rate of UL in China is slightly higher than that of the UP. However, the IGULUP vary according to development stages. UAs in the primary stage and the early growth stage face the dilemma where UL grows faster than the UP. Conversely, for UAs in the later growth stage and the mature stage, the growth rate of the UP is higher than that of UL. Finally, an increase in economic development level, population agglomeration, fiscal expenditure, and urban compactness can help mitigate the gap between UL and UP. In contrast, industrial structure, urbanization level, and foreign direct investment may hinder the improvement of IGULUP by accelerating the rate of land expansion. These findings may make theoretical contributions to the formulation of more targeted land use control policies and urban population growth strategies.
1H NMR Chemical Shift Changes as a Result of Introduction of Carbonyl-Containing Protecting Groups Observed in Bornol and Isoborneol
Authors: Lyu Baohe , Sako Honoka , Sugiura Mio , Hiraga Yoshikazu , Takagi Ryukichi , Niwayama Satomi
Published: October 2024

Complete assignments of the 1H NMR chemical shifts for monoterpenes, borneol, and isoborneol, and their derivatives in which the secondary hydroxy group is protected with an acetyl group or a benzoyl group, have been made in CDCl3 and C6D6. Upon the protection of the hydroxy group with the carbonyl functional groups, or acetyl or benzoyl groups, many protons constituting the bicyclic ring exhibited downfield and upfield shifts in the chemical shift values, aiding in the unambiguous assignments of protons and carbons.
Concanavalin a Grafted Nanoemulsions for Nasal Delivery: Preliminary Studies with Fluorescently Labelled Formulations
Authors: Mışraklı Merve , Rizzo Sebastiano Antonio , Bordano Valentina , Bozza Annalisa , Ferraris Luca , Marini Elisabetta , Muntoni Elisabetta , Capucchio Maria Teresa , Scomparin Anna , Battaglia Luigi
Published: October 2024

Nasal delivery is a non-invasive strategy for effective drug delivery. Nevertheless, in order to promote drug uptake by the nasal mucosa, it is fundamental to increase its residence time in the administration site. To this aim, nano-sized drug delivery systems are widely exploited. Within this context, the commercially available nanoemulsion for parenteral nutrition is a biocompatible, safe and clinically approved vehicle for drug delivery. Furthermore, the nanodroplet surface can be modified via a well-established protocol to graft Concavalin A, a lectin capable of improving the mucosal adhesion, by binding to the α-mannose and α-glucose residues of the mucosal glycocalyx. The obtained targeted formulation is able to induce haemagglutination, as opposite to non-modified nanoemulsion. Furthermore, the ConA grafting maintains the physicochemical properties of the nanodroplets (size~230 nm, Z < −35 mV) and does not interfere with the loading of the Rose Bengal fluorescent probe. Fluorescently labelled ConA grafted nanodroplets showed enhanced permeation and accumulation in ex vivo bovine nasal mucosa. This study is a proof of concept that Concanavalin A can be used to decorate the surface of nanodroplets, acting as a permeation promoter.
Effect of Tempering Temperature on the Aqueous Corrosion Resistance of 9Cr Series Heat-Resistant Steel
Authors: Li Hui , Bai Hao
Published: October 2024

In this investigation, the aqueous corrosion resistance of 9Cr series heat-resistant steel during tempering was investigated. Optical Microscopy (OM), Scanning Electron Microscopy (SEM), and Energy Dispersive Spectrometer (EDS) were used to analyze the effect of tempering temperature on the microstructure and precipitation behavior of precipitates. The heat-resisting steel was heated to 1150 °C for 1 h, and then tempered at different temperatures between 680 °C and 760 °C for 2 h. The microstructure of the heat-resistant steel after tempering was composed of lath-tempered martensite and fine precipitates. The hardness decreased with increasing tempering temperature, ranging from HBW 261 to HBW 193. The aqueous corrosion resistance improved as the tempering temperatures increased from 680 °C to 720 °C but deteriorated at higher temperatures, such as 760 °C, which was obtained by an electrochemical corrosion performance test. The aqueous corrosion resistance was affected by the decrease in dislocation density and the decrease in Cr solution in the tempered martensite. With the increase in the tempering temperature, the aqueous corrosion potential first increases and then decreases, the self-corrosion current density first decreases and then increases, and the polarization resistance first increases and then decreases. Furthermore, the increase in corrosion resistance is attributed to the reduction in dislocation density and chromium depletion in the martensitic structure as the tempering temperature approaches 720 °C. This paper reveals the effect of tempering temperature on the corrosion resistance of 9Cr series heat-resistant steel, which is a further exploration of a known phenomenon.
Synthesis and Properties of Highly Tilted Antiferroelectric Liquid Crystalline (R) Enantiomers
Authors: Urbańska Magdalena , Zając Monika , Czerwiński Michał , Morawiak Przemysław , Bubnov Alexej , Deptuch Aleksandra
Published: October 2024

This work reports the synthesis method and various properties of four rod-like antiferroelectric (R) laterally substituted enantiomers, with or without fluorine atoms used as substituents in the benzene ring. The influence of fluorine substitution on the mesophase temperature range was determined. The synthesized compounds are three-ring rod-like smectics with a chiral center based on (R)-(−)-2-octanol. Their chemical and optical purity was checked using high-performance liquid chromatography (HPLC). Two newly synthesized enantiomers and three previously reported (R) enantiomers were used to formulate two antiferroelectric mixtures. The mesomorphic behavior was characterized by polarizing optical microscopy, differential scanning calorimetry, and X-ray diffraction (XRD). The helical pitch and tilt angle measurements were done using the selective light reflection phenomenon and the electro-optical method, respectively. All the enantiomers exhibit a wide temperature range of the antiferroelectric phase, with a high tilt angle. Furthermore, the enantiomer with lateral fluorine substitution in the ortho position has a very long helical pitch (more than 2.0 µm), relatively low enthalpy of melting point, and a tilt angle close to 45 degrees. The designed (R) enantiomers can be useful for formulating eutectic mixtures for further use in various devices, including photonics and optoelectronics.
Effect of Initial Surface Morphology and Laser Parameters on the Laser Polishing of Stainless Steel Manufactured by Laser Powder Bed Fusion
Authors: Liu Jiangwei , Zhao Kangkang , Wang Xiebin , Li Hu
Published: October 2024

The topological characteristics of the down-skin surfaces for as-built components by laser powder bed fusion (LPBF) are particularly representative, while the study on the improvement of the surface quality of these surfaces remains largely unexplored. Herein, the laser polishing of LPBF-built components with different inclination angles was systematically investigated with an emphasis on the down-skin surfaces. Our result shows that the topography of the top surface is independent of the inclination angle, and the surface topography of the down-skin surface is dominated by additional angle-dependent surface characteristics. It also indicates that the surface roughness can be reduced sharply when increasing the laser power from 40 W to 60 W, and the reduction slows down when further increasing the laser power while decreasing the scanning speed leads to a progressive improvement of the surface morphology. Moreover, a second-order regression model was established to evaluate the influence of the initial surface morphology and polishing parameters on the polished surface roughness and to achieve surface roughness optimization. Therefore, our established methodology can be readily applied to surface morphology manipulation and process optimization for laser polishing of widely used metals and alloys fabricated by the additive manufacturing process.
Facile Incorporation of Carbon Nanotubes into the Concrete Matrix Using Lignosulfonate Surfactants
Authors: Kostrzanowska-Siedlarz Aleksandra , Musioł Krzysztof , Ponikiewski Tomasz , Janas Dawid , Kampik Marian
Published: October 2024

One of the ways to turn concrete into smart concrete involves the incorporation of conductive fillers. These fillers should be evenly distributed in the matrix to enable the charge propagation necessary for sensing. To homogenize the mixture, typical surface-active chemical compounds are routinely employed. Unfortunately, their presence often negatively impacts the characteristics of concrete. In this work, we show that conductive multi-walled carbon nanotubes (MWCNTs) can be included in the concrete matrix by using off-the-shelf lignosulfonate-based plasticizers. These plasticizers showed a much-improved capability to disperse MWCNTs compared to other routinely used surfactants. They also prevented a significant deterioration of the consistency of the mixture and inhibited the acceleration of the hydration process by MWCNTs. In concretes with MWCNTs and lignosulfonate-based plasticizers, the mechanical properties were largely preserved, while the nanocomposite became electrically conductive. Consequently, it enabled evaluation of the condition of the material by electrical impedance measurements.
Simulation of Friction Stir Welding of AZ31 Mg Alloys
Authors: Feng Sili , Liu Zhe , Xin Renlong
Published: October 2024

Friction stir welding has been extensively applied for the high-quality bonding of Mg alloys. The welding temperature caused by friction and plastic deformation is essential for determining the joint characteristics, especially the residual stress and weld microstructure. In this work, a modified moving heat source model was proposed by considering the variations in heat generation caused by friction shear stress at both the side and bottom surfaces of the tool. The application of this model was further extended to the entire welding process, especially in the plunging stage. The relative errors between the experimental and simulated peak temperatures at characteristic points were small, with a maximum of 10%, thereby validating the model for accurate temperature prediction. Furthermore, the influence of welding and rotational speed on temperature fields was systematically investigated. At relatively low welding and rotational speeds, the welding temperature increased significantly with either an increase in rotational speed or a decrease in welding speed. However, this effect gradually diminished at higher welding and rotational speeds. These results provide some valuable guidelines for controlling heat generation to improve the quality of Mg alloy welds.