bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Coupled Arctic Forecast System’s representation of the Arctic atmospheric boundary layer vertical structure during MOSAiC
Authors: Jozef Gina , Cassano John J. , Solomon Amy , Intrieri Janet , de Boer Gijs
Published: July 2024

Observations from the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) were used to evaluate the Coupled Arctic Forecast System (CAFS) model’s ability to simulate the atmospheric boundary layer (ABL) structure in the central Arctic. MOSAiC observations of the lower atmosphere from radiosondes, downwelling longwave radiation (LWD) from a pyranometer, and near-surface wind conditions from a meteorological tower were compared to 6-hourly CAFS output. A self-organizing map (SOM) analysis reveals that CAFS reproduces the range of stability structures identified by the SOM trained with MOSAiC observations of virtual potential temperature (θv) profiles, but not necessarily with the correct frequency or at the correct time. Additionally, the wind speed profiles corresponding to a particular θv profile are not consistent between CAFS and the observations. When categorizing profiles by static stability, it was revealed that CAFS simulates all observed stability regimes, but overrepresents the frequency of near-surface strong stability, and underrepresents the frequency of strong stability between the top of the ABL and 1 km. The 10 m wind speeds corresponding to each stability regime consistently have larger values in CAFS versus observed, and this offset increases with decreasing stability. Whether LWD is over or underestimated in CAFS is dependent on stability regime. Both variables are most greatly overestimated in spring, leading to the largest near-surface θv bias, and the greatest underrepresentation of strong stability in spring. The results of this article serve to highlight the positive aspects of CAFS for representing the ABL and reveal impacts of misrepresentations of physical processes dictating energy, moisture, and momentum transfer in the lower troposphere on the simulation of central Arctic ABL structure and stability. This highlights potential areas for improvement in CAFS and other numerical weather prediction models. The SOM-based analysis especially provides a unique opportunity for process-based model evaluation.
GEOLOGICAL STRUCTURE AND PROSPECTS OF HYDROCARBON POTENTIAL OF PALEOZOIC DEPOSITS IN SOUTHEASTERN REGIONS OF WEST SIBERIA
Authors: КОНТОРОВИЧ В.А. , КОНТОРОВИЧ А.Э. , АЮНОВА Д.В. , ИБРАГИМОВА С.М. , БУРШТЕЙН Л.М. , КАЛИНИН А.Ю. , КАЛИНИНА Л.М. , КАНАКОВА К.И. , КОСТЫРЕВА Е.А. , СОЛОВЬЕВ М.В. , ФИЛИППОВ Ю.Ф.
Published: July 2024

Статья посвящена анализу геологического строения и перспективам нефтегазоносности палеозойских отложений юго-восточных районов Западной Сибири, включающих Томскую, Новосибирскую, Омскую области и юго-запад Красноярского края в левобережной части Енисея. В процессе проведенных исследований проанализировано тектоническое строение фундамента, сделан вывод о том, что большую часть исследуемой территории занимает Центрально-Западно-Сибирская зона поздних герцинид, на северо-востоке Томской области и прилегающей территории Красноярского края развит Предъенисейский верхнепротезойско-палеозойский осадочный суббасейн, залегающий на добайкальском фундаменте; построены регионально-зональные модели рифей-кембрийских отложений Предъенисейской субпровинции и палеозойских отложений в зоне распространения герцинид; проанализирован вещественный состав и возраст пород доюрского основания; выполнен анализ перспектив нефтегазоносности верхнепротерозойско-палеозойских отложений, выделены нефтегазоперспективные блоки и объекты; разработаны сейсмогеологические критерии выявления и детального картирования палеозойских нефтегазоперспективных объектов; построены сейсмогеологические модели эталонных месторождений.

In this paper we analyze the geological structure and prospects of hydrocarbon potential of the Paleozoic deposits in southeastern regions of West Siberia, including the Tomsk, Novosibirsk, Omsk regions and the southwest of the Krasnoyarsk Territory in the left-bank part of the Yenisei River. In the process of our studies, we analyzed the tectonic structure of the basement and made a conclusion that most of the studied area is occupied by the Central West Siberian zone of Late Hercynides. In the northeast of the Tomsk region and the adjacent area of the Krasnoyarsk Territory, the cis-Yenisei Upper Protezoic–Paleozoic sedimentary subbasin, lying on the pre-Baikal basement, developed. We constructed regional-zonal models of the Riphean–Cambrian deposits of the cis-Yenisei subprovince and Paleozoic deposits in the area of distribution of Hercynides and analyzed the material composition and age of the rocks of the pre-Jurassic basement. We assessed hydrocarbon potential of the Upper Proterozoic–Paleozoic deposits, identified prospective blocks and objects, developed seismogeological criteria to identify and map in detail the Paleozoic oil and gas prospective reservoirs and constructed seismogeological models of reference fields.
Next-generation sequencing as a valuable tool for mutational spectrum in advanced-stage NSCLC patients
Authors: Iurca Ioana , Isakescu Ecaterina , Pop Laura Ancuta , Budisan Liviuta , Pirlog Radu , Harangus Antonia , Ciuleanu Tudor Eliade , Braicu Cornelia , Berindan-Neagoe Ioana
Published: July 2024

Background and aim. Lung cancer remains one of the most threatening malignancies, ranking as the second most diagnosed cancer, and it continues to be the leading cause of cancer-related deaths worldwide. Challenges persist with late diagnosis and the high mutational burden characteristic of lung cancer.
Methods. Our study focuses on identifying the mutational spectrum of a cohort of advanced-stage non-small cell lung cancer (NSCLC) patients using a minimally invasive method through blood collection. To analyze the mutational landscape of these patients, we employed plasma DNA for the next-generation sequencing (NGS) cancer panel Ion Torrent, which contains 50 of the most mutated genes in lung cancer. All protocols for extraction, quality and quantity control, and library preparation follow the manufacturer’s rules. Bioinformatics analysis was performed to select pathogenic mutations versus non-pathogenic-benign ones.
Results. This approach is particularly valuable for patients in advanced stages (III and IV, n=10) of lung adenocarcinoma and lung squamous cell carcinoma, who lack surgical options and limited therapeutic avenues. The comprehensive sequencing analysis revealed that nine of the ten lung cancer patients carried a TP53 mutation. Also, several other mutations exist in various cases, showing heterogeneous profiling.
Conclusions. Our findings demonstrate the potential of liquid biopsies in providing crucial genetic insights that can guide personalized treatment strategies, improving the management and outcomes for patients with advanced lung cancer.
Dentists’ stress level during the COVID-19 pandemic and their opinion on the oral health status of their patients
Authors: Paun Ariadna Georgiana Eugenia , Bolboacă Sorana D. , Chifor Radu , Radu Constantin , Strilciuc Ștefan , Badea Iulia Clara , Borzan Cristina Maria
Published: July 2024

Background and aims. “Severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2) is the name of the etiological agent of the pandemic Corona Virus Disease (COVID-19) which was declared on March 11, 2020 by the WHO and which affected all countries of the world, including Romania. Our study aimed to evaluate the psycho-affective implications and economic consequences for dentists in Romania during the COVID-19 (Corona Virus Disease) pandemic state of emergency and their opinion on the patients’ oral health impact.
Methods. A cross-sectional observational and analytical study based on a questionnaire was conducted. Dentists who work in Romania affiliated in 2020 to the Romanian College of Dental Practitioners was the target population.
Results. Three hundred and seventy-three dentists participated in this survey. The age of the respondents ranged from 24 to 70. Men and women have the same expectation regarding the lockdown effect of worsening the oral health of the general population (worst, 79.5% of women and 81.3% of men, P=0.8842).
Conclusions. The anxiety regarding the bank rates, supplementary investments, risk of COVID-19 infection and exposure to COVID-19 related mass-media information proved to be significantly higher among young dentists.
Routine Postclinical Event Debriefings on Inpatient Pediatric Units
Authors: Bohorquez Jenny , Patel Amee D. , Borders Rachel , Gorman April , Reynolds Chelsea , Ritchie Kristin , Denson Natalie , Solomon Courtney M.
Published: July 2024

BACKGROUND AND OBJECTIVES

Debriefings are an underutilized opportunity to enhance team performance and safety culture. Little is known about the impact of postclinical event debriefing programs in Pediatric Hospital Medicine (PHM). We sought to develop a standardized debriefing process with multidisciplinary involvement after all clinical events on PHM service lines. Our primary aim was to achieve 75% debriefing completion rate over 12 months with debriefing duration less than 10 minutes.

METHODS

A standardized postclinical event debriefing process was created at a large tertiary children’s hospital. We aimed to debrief after clinical events on PHM services. The debriefing process was developed with key stakeholders and used a key driver diagram and Plan-Do-Study-Act cycles to refine the process. The project team reviewed the data monthly.

RESULTS

During our 20-month study period, debriefing completion rate sustained a median of 66% with a median debriefing time of 7 minutes. Most debriefings (61%) had all core team members present with attending physicians (pediatric hospitalists) being absent most often. Barriers to debriefing with all core members present included service type, time of day, and shift change. Process changes were implemented based on concerns addressed in the debriefings.

CONCLUSIONS

Multidisciplinary, postclinical event debriefings were successfully implemented on inpatient pediatric wards. Future steps include process implementation on non-PHM units in our hospital based on expressed interest and to further assess how debriefings optimize team performance and improve clinical outcomes.
Quality Improvement Interventions to Prevent Intraventricular Hemorrhage: A Systematic Review
Authors: Edwards Erika M. , Ehret Danielle E.Y. , Cohen Howard , Zayack Denise , Soll Roger F. , Horbar Jeffrey D.
Published: July 2024

OBJECTIVES

Quality improvement may reduce the incidence and severity of intraventricular hemorrhage in preterm infants. We evaluated quality improvement interventions (QIIs) that sought to prevent or reduce the severity of intraventricular hemorrhage.

METHODS

PubMed, CINAHL, Embase, and citations of selected articles were searched. QIIs that had reducing incidence or severity of intraventricular hemorrhage in preterm infants as the primary outcome. Paired reviewers independently extracted data from selected studies.

RESULTS

Eighteen quality improvement interventions involving 5906 infants were included. Clinical interventions in antenatal care, the delivery room, and the NICU were used in the QIIs. Four of 10 QIIs reporting data on intraventricular hemorrhage (IVH) and 9 of 14 QIIs reporting data on severe IVH saw improvements. The median Quality Improvement Minimum Quality Criteria Set score was 11 of 16. Clinical intervention heterogeneity and incomplete information on quality improvement methods challenged the identification of the main reason for the observed changes. Publication bias may result in the inclusion of more favorable findings.

CONCLUSIONS

QIIs demonstrated reductions in the incidence and severity of intraventricular hemorrhage in preterm infants in some but not all settings. Which specific interventions and quality improvement methods were responsible for those reductions and why they were successful in some settings but not others are not clear. This systematic review can assist teams in identifying potentially better practices for reducing IVH, but improvements in reporting and assessing QIIs are needed if systematic reviews are to realize their potential for guiding evidence-based practice.
A importância da sinodalidade na Igreja
Authors: Aguiar Nobre José , Gonçalves Costa Everton , De Almeida Amaral Emerson
Published: July 2024

A presente pesquisa visa destacar a importância da sinodalidade na vida da Igreja, como elemento fundamental para sua ação evangelizadora no mundo de hoje. Trata-se de uma pesquisa bibliográfica, cujo problema ficou assim formulado: como facilitar uma visão eclesiológica em sintonia com o Magistério da Igreja em face aos desafios do presente? Esse tema já esteve presente nas primeiras comunidades cristãs, foi evidenciado pelo Vaticano II e voltou aos círculos de reflexão, de modo forte e vigoroso, no pontificado do Papa Francisco. Entendemos que pensar uma Igreja sinodal é fundamental hoje, pois envolve toda a visão eclesiológica atual na dinâmica pastoral e os desafios hoje. Por isso, esse tema se torna importante para a formação dos futuros presbíteros, sobretudo ligados à dimensão pastoral-missionária, proposta pelas diretrizes para a formação dos presbíteros da Igreja no Brasil. Os resultados esperados buscarão sinais de sinodalidade no texto das diretrizes e no processo formativo como um todo, a fim de destacarmos pistas e meios de aplicação concreta da dinâmica sinodal na vida dos formandos ao longo de todo o processo.
A felicidade no conceito bíblico e na pós-modernidade
Authors: Gonzaga Waldecir , Dantas da Silva Júnior Marcelo
Published: July 2024

A construção mercadológica da sociedade ocidental pós-moderna estabeleceu os parâmetros da “felicidade” na efemeridade e na manipulável sensação de bem-estar dos indivíduos. Muito disso ocorreu devido à secularização e ao rompimento com alguns valores até outrora muito importantes para a civilização. Na contramão dessa perspectiva, este artigo tem como objetivo aduzir e resgatar o conceito de “felicidade” a partir da cosmovisão cristã. Para isso, recorre-se ao texto de 1Pd 3,13-17, no qual há valiosos princípios vivenciais cuja alusão à transcendentalidade é notória. Através de uma análise exegética do texto e de uma revisão bibliográfica sobre o tema da “felicidade”, observa- -se como a “felicidade” na ótica cristã traz benefícios para o indivíduo e para a sociedade. A escolha do texto de 1Pd 3,13-17 dá-se pelo tema da “felicidade” (v. 14: “μακάριοι/felizes”) e pelo diálogo entre Teologia e Bíblia, proporcionando um impacto na espiritualidade nas duas dimensões: vida pessoal e vida comunitária. Assim, dá testemunho da esperança cristã (v. 15), seguindo a “boa conduta de Cristo” (v. 16), praticando a “vontade de Deus” (v. 17), promovendo o bem de forma incansável, mesmo em face de todos os desafios. Para tanto, apresenta-se o texto bíblico em grego e a tradução portuguesa, trazem-se notas de crítica textual, dados gerais da carta e uma abordagem sobre a temática “felicidade” na pós-modernidade, abrindo o leque para ulteriores pesquisas.
Assessing the cognitive salience of wild fauna in the Barranca de Metztitlán Biosphere Reserve, Mexico
Authors: Zepeda Hernández Zeltzin Ketzalken , Pulido Silva María Teresa , Sánchez Rojas Gerardo
Published: July 2024

It is important to document the knowledge possessed by rural people about the species of flora and fauna in their environment, so that this knowledge can be incorporated into conservation efforts. We set out to learn about the traditional knowledge held by the inhabitants of the Barranca de Metztitlán Biosphere Reserve (BMBR) regarding the fauna in their environment, identifying which wild species they recognize and what cognitive salience they assign to them. We also compared knowledge between people who live in the valley (La Vega) and the mountains (La Sierra), and between men and women to determine whether knowledge was different. We conducted semi-structured interviews from April 2016 to February 2017. In order to evaluate traditional knowledge, we used the Smith index because it combines the frequency and order of mention of the listed elements. We found that in the BMBR local people recognized 37 wild species, including 25 mammals, 9 birds, two reptiles and one unidentified species. The Smith Index is only correlated with mention frequency, but not with mention order. The ethnozoological knowledge is structured by gender; while men mention 100% species of the list generated by the interviews, women only cover 59.5%. Some wild animals recorded a significant variation in the cognitive salience between La Vega and La Sierra. The local population has extensive knowledge about which wild species inhabit the area, mainly of mammals and secondarily of birds and reptiles. This knowledge should be integrated into conservation plans for the reserve.
La frontera el «No-lugar» recurrente en la literatura mexicana reciente. Un breve recorrido desde los márgenes
Authors: Castillo-Carrillo Gerardo
Published: July 2024

El presente artículo se centra en analizar algunas obras clave de la literatura mexicana contemporánea, en las que la frontera geográfica sur o norte de México es representada como un espacio contrahegemónico, violento y geopolíticamente vulnerable. Para tal propósito, se revisarán de manera particular las novelas 2666 (2004), de Roberto Bolaño; Al otro lado (2008), de Heriberto Yépez; Las tierras arrasadas (2015), de Emiliano Monge, así como el poemario testimonial El libro centroamericano de los muertos (2018), de Balam Rodrigo. En estos textos la frontera es configurada como un territorio de múltiples relaciones socioculturales y transacciones económicas, en los que los valores e intereses particulares están condicionados por las leyes del mercado. En cada una de estas obras está presente de manera directa o indirecta el narcotráfico, el cual controla, administra o elimina todo aquello que le parece inservible.
Myeloma multiplex: a jelen kihívásai és a korszerű ellátás feltételei I.
Authors: Mór Zoltán , Tóth Ágnes , Alizadeh Hussain , Borbényi Zita , Egyed Miklós , Illés Árpád , Masszi Tamás , Mikala Gábor
Published: July 2024

A myeloma multiplex a kórosan átalakult, klonális plazmasejtek csontvelői szaporulata, ami az érett, perifériás B-sejtes neoplazmák közé tartozik. A myeloma multiplex a második leggyakoribb onkohematológiai kórkép. A hatékonyabb terápiás lehetőségeknek köszönhetően a betegek átlagos túlélése jelentősen nőtt: a korábbi 4–5 évhez képest a várható túlélés 6–8 évre becsülhető, jobb prognózisú betegek esetében a 10 évet is elérheti. Magyarországon, az epidemiológiai és túlélési adatok alapján a myeloma multiplex miatt kezelt betegek várható száma 2500–3000-re becsülhető.

A Magyarországon jelenleg kezelt nagy betegszám egyrészt közvetett módon alátámasztja a folyamatosan javuló túlélési eredményeket, másrészt felhívja a figyelmet a hosszan tartó MM ellátás okozta új kihívásokra is: 1) hosszabb terápiás időtartamokra, továbbá 2) egyre több beteg megjelenésére a fekvőbeteg- és járóbeteg-szakellátásban, így az ellátórendszer már rövid távon is megjelenő kapacitásproblémáira.

Felismerve a myelomaellátásban rejlő kihívásokat, a Myeloma multiplex: a jelen kihívásai és a korszerű ellátás feltételei áttekintő tanulmány elkészítésével célunk egy rendszerszemléletű javaslati anyag kidolgozása volt, amely az ellátás minden területét érintve átfogó értékelést nyújt, kitérve a lehetséges beavatkozási pontokra. 2023-ban széles körű szakmai elemzés készült, amely a nemzetközi ajánlásokat és klinikai eredményeket is figyelembe véve értékeli a hazai myelomaellátást az új kihívások, szűk keresztmetszetek és klinikai gyakorlat szempontjából.

Az elemzés egy 20 főből álló – a hazai myelomaellátást képviselő – szakértői csoport véleményét feldolgozva értékeli a hazai terápiás gyakorlatot és javaslatokat fogalmaz meg a myelomaellátás fejlesztése érdekében.

Jelen összegzés a Tanulmány eredményeire alapozva mutatja be a myeloma multiplex korszerű kezelési elveit, a Magyarországon elérhető terápiás lehetőségek körét, továbbá a kielégítetlen szükségletet. Célunk, hogy az érvényes nemzetközi ajánlásokkal és klinikai eredményekkel összehasonlítva vizsgáljuk a myelomaterápiák közfinanszírozott hozzáférhetőségét Magyarországon, a feltárt szűk keresztmetszeteket, és bemutassuk – nemzetközi összevetésben – a kielégítetlen gyógyszerterápiás szükségletet.
Alexis Gauthier: Vegetronic
Authors: Richardson Alice
Published: July 2024

Alexis Gauthier: Vegetronic: Preface, 2013: 375 pp., hardback, £30.00.
Empathy and Self-Empathy in the Anthropological Dimension of Modernity
Authors: Shabanova Y. O.
Published: July 2024

Purpose. In the article, the author questions rethinking the phenomena of empathy and self-empathy as modes of self-understanding of humanity and the inner intention to self-exploration of human spirituality. Theoretical basis. The research is based on the phenomenological dimension of modern anthropology and axiology. Originality. The change of the traditional intersubjective approach in the understanding of empathy to an introsubjective one and the affirmation of self-empathy as one of the defining existences in human beings, which is adjusted by the altruism-egoism value scale and forms the skills of unconditional empathic action towards the Other as a mode of self-exploration in the spiritual essence of a person are argued. Conclusions. The commonplace in a wide palette of research views on empathy is the construct for the Self – Other disposition and the intersubjective approach emphasizing the object of empathy. Transformation of the intention vector of empathic consciousness from the object to the subject of empathic action opens up deep possibilities of spiritual improvement as a way of self-awareness in the human essence. The transformation of the intention vector of empathic consciousness from intersubjective (I in the Other) to introsubjective (I through the Other) has the potential for spiritual development according to the altruism-egoism value scale. Empathy as an introsubjective position of self-exploration is inseparably connected with self-empathy, which in the mutual integration of its three main components – benevolence towards oneself, reflexivity towards one’s own experience and a tendency to understand one’s fate as common to all mankind – forms an important regulator of self-understanding of a person’s spiritual potential and the possibility of the practical extrapolation for these skills to an empathic way of communication at all levels of anthropo-being. Anthropological accentuation on the inner essence and meanings of human existence is carried out through intuition and self-altruism as the fading of pragmatic interest in the socially conditioned Self (Ego) and immersion in the essential Self – the bearer of the universal spiritual in a person. Self-empathy is considered in the context of the ideas of Stoicism as a deep self-immersion in the human essence and sensitivity to immediate emotional internal states, as a construct of emotional intelligence and a way of realizing individual uniqueness in the context of the metamodern concept of atopy. Self-empathy is defined as one of the key existences in human beings, which forms the skill of altruistic (unconditional) implementation of empathy.
Communicative Dimension of Human Freedom under Deliberative Democracy
Authors: Drapushko R. G.
Published: July 2024

Purpose. This article aims to analyse the ways of free communicative solution of civil society problems as a basis for the development of deliberative democracy on the example of the activities of volunteer organisations. Theoretical basis. The conceptual basis of the study is Immanuel Kant’s philosophical understanding of individual obligations as the basis for the institutionalisation of social communication. This concept is developed by Jürgen Habermas in the direction of deliberative democracy. Max Weber, Quentin Skinner, and other theorists give a special status to language communication in the functioning of social institutions. Contemporary Ukrainian researchers analyse the volunteer movement as the basis for the communicative implementation of human freedom. Originality. Practices of political performance in society contribute to the specific and contextual solution of the tasks of communication theory and provide answers to questions about sustainable human needs, on the one hand, and, on the other hand, contribute to solving the political problems of everyday life. Grassroots socio-political movements, and especially volunteer movements, practice free discussion of various social problems, which is the basis of the deliberative democracy. Conclusions. Overcoming value differences between members of society is more effective in deliberative practices as the core of social communication in a democratic society. The substantive way to find new forms of communication is to use deliberative practices of implementing freedom to improve the functioning of social institutions. The formal way is aimed at attracting the experience of volunteer organisations in using the minimum necessary forms of bureaucratisation of social institutions in a democratic society.
Anthropological Dimension of Wartime Ecocide: Ecofeminist Methodological Assessments
Authors: Karpenko K. I. , Hagengruber R. E. , Nielsen C. R.
Published: July 2024

Purpose. The authors aim to disclose the anthropological dimension of ecocide during and after Russia’s war against Ukraine, relying on the multidisciplinary practices and intellectual production of ecofeminist women thinkers, including philosophers, sociologists, historians, psychologists, and others. The theoretical basis methodological approaches in philosophical anthropology, phenomenology, analytical philosophy, communicative philosophy, existentialism, ethics of justice, and ethics of care determine the study’s theoretical basis. Originality. For the first time, a systematic analysis of the anthropological dimension of ecocide has been carried out based on ecofeminist methodology. The specifics of the Ukrainian resistance to ecocide were revealed as part of a single struggle – an anti-imperial and ecological struggle for independence and prosperity. It is emphasized that during the war, the opposition to ecocide is a component of the fight for national sovereignty and territorial integrity of the country, and therefore, the ideas of pacifism in this period are subordinated to this primary goal. After the war, preventing threats of ecocide should become an integral part of the struggle for stable peace and prosperity. Ecofeminist pacifist perspectives should be a priority among the most essential goals for that period. Conclusions. Ecofeminist methodological assessments offer valuable insights into the anthropological dimension of ecocide in wartime and post-war contexts, highlighting the complex interplay between gender, violence, and environmental destruction. By focusing on the experiences and perspectives of women and marginalized communities, ecofeminist analyses contribute to a more holistic understanding of ecocide and its impacts on both human populations and ecosystems.
Challenges of obtaining cultured corneal endothelial cells for regenerative purposes
Authors: Ostrovski D. S. , Borzenok S. A. , Malyugin B. E. , Antonova O. P. , Khubetsova M. Kh. , Kerimov T. Z.
Published: July 2024

Human posterior corneal epithelium (corneal endothelium) has limited proliferative activity both in vivo and in vitro. Disease or dysfunction in these cells leads to impaired corneal transparency of varying degrees of severity, up to blindness. Currently, the only effective standard treatment for corneal endothelial dysfunction is transplantation of donor cornea that contains a pool of healthy and functionally active cells. However, there is a global shortage of donor corneas, which has led to an unmet clinical need and the fact that only 1 patient out of 10 in need receives surgical treatment. Therefore, creation of cellular constructs and artificial human corneas containing healthy endothelium is a very urgent challenge facing modern ophthalmic transplantology. This review presents the current state of affairs, challenges and prospects for obtaining cultured corneal endothelial cells (CECs) in vitro for transplantation purposes.
Endoscopic full-thickness resection of sigmoid colon cancer in a liver recipient
Authors: Bekov M. T. , Monakhov A. R. , Smirnov K. S. , Yakunin Ya. S. , Latypov R. A. , Oleshkevich D. O. , Tsirulnikova O. M. , Gautier S. V.
Published: July 2024

Compared with the general population, solid organ transplant recipients have a higher cancer risk. This is mainly due to the use of immunosuppressive therapy. Colorectal cancer is one of the most common cancers in recipients. This paper presents the experience of endoscopic full-thickness resection (EFTR) of a sigmoid colon cancer in a liver recipient.
Cardiac allograft vasculopathy: current review
Authors: Mironkov B. L. , Uvarova D. D. , Koloskova N. N. , Sapronova Yu. V. , Muminov I. I. , Yusova A. A. , Sakhovsky S. A.
Published: July 2024

Transplant coronary artery disease (TCAD) is one of the main causes of graft dysfunction and graft loss. Early diagnosis and treatment of cardiac allograft vasculopathy (CAV) can increase graft survival and improve the prognosis for heart transplant recipients. This review presents current data on the problem of CAV, its pathogenesis and the main factors influencing the course of this disease.
Optimal strategies for prevention of ischemia‑reperfusion injury in heart transplantation with prolonged cold ischemia time (review)
Authors: Fomichev A. V. , Garmaev G. B. , Zhulkov M. O. , Zykov I. S. , Makaev A. G. , Protopopov A. V. , Tarkova A. R. , Murtazaliev M. N. , Smirnov Ya. M. , Limanskiy A. D. , Guseva A V. , Kaldar K. N. , Sirota D. A.
Published: July 2024

The shortage of organs for transplant remains a major challenge in transplantology. Transporting donor organs over long distances increases cold ischemia time, which is a risk factor for ischemia-reperfusion injury (IRI). In the face of critical shortages, the method and timing of organ preservation are crucial in increasing the donor pool. This paper examines the approaches, benefits, and drawbacks of organ preservation techniques used around the world.
Management and Outcomes of Aneurysms Found in Deceased Donor Livers: A Review of Published Cases
Authors: González De Godos A. , Pérez Saborido B. , Bailón Cuadrado M. , Pacheco Sánchez D.
Published: July 2024

Introduction. The organ shortage has prompted transplant surgeons to accept grafts from deceased donors, which can lead to complex reconstructions. The presence of an aneurysm can complicate the arterial anastomosis of the liver transplant, leading to postoperative vascular complications such as hepatic artery thrombosis or stenosis.

Objective: review published cases of donor liver aneurysms and their management.

Materials and methods. After an exhaustive literature search, only 4 published cases of liver transplants from grafts with aneurysms in their vascular territory have been found.

Results. These vascular anomalies were corrected by vascular reconstructions and no postoperative arterial complications were observed.

Conclusion. Although no particular arterial configuration precludes the use of a donor liver for transplant, more arterial complications can be anticipated with complex arterial reconstructions. However, properly managed arterial anomalies do not necessarily compromise graft outcome. Therefore, our review of the literature shows the possibility of using these organs for liver transplantation, which would otherwise be discarded.
Tateando a célula: produção de modelo tátil no ensino de Biologia para alunos com deficiência visual
Authors: Neiva Renata Sarkis , De Siqueira Andréa Espinola , De Mattos José Carlos Pelielo
Published: July 2024

As pessoas com deficiência visual demandam práticas educativas diferenciadas e inclusivas. São escassos os trabalhos na literatura sobre Educação Inclusiva para estudantes com deficiência visual no ensino de biologia. Buscando facilitar e dinamizar os processos de ensino aprendizagem da disciplina, esse trabalho teve como objetivo produzir um modelo tátil sobre o tema citologia e um tutorial para replicação e utilização no âmbito da deficiência visual, no Ensino Médio do Centro de Educação de Jovens e Adultos Instituto Benjamin Constant (CEJA-IBC), no Rio de Janeiro. O produto foi confeccionado pela professora responsável pela disciplina e foi testado e avaliado por professores e técnicos da instituição e, posteriormente, aplicado em oficina presencial para alunos com deficiência visual. Este recurso facilitou a abordagem da temática citologia no âmbito da deficiência visual.
Policy Matters: Tendencies towards Academic Misconduct
Authors: Erçin Kamburoğlu Nalan , Razı Salim
Published: July 2024

Since the outbreak of COVID-19 in March 2020, the global education system has been compelled to adopt Emergency Remote Teaching (ERT) in response to the threat of the virus. This abrupt shift in teaching methods has yielded anticipated and unanticipated consequences across all levels of education. The objective of this study is to investigate the impact of ERT on academic integrity at a higher education institution situated in the western region of Türkiye. 12 hypothetical academic misconduct scenarios questionnaire was completed by a total of 234 participants. The responses relating to student observations suggest that collusion is perceived to be the most commonly witnessed form of academic misconduct, with many students specifically noting the prevalence of exam outsourcing. However, contract cheating was found to be the least preferred method of academic transgression in this study. Another significant finding suggests based on students observations that in-class activities facilitated by teachers can inadvertently facilitate either collaboration or dishonest practices among students during ERT. It is worth emphasizing that the implementation of well-designed assignments for effective assessments can serve as an initial step towards preserving academic integrity within an academic institution. Consequently, this study underscores the persisting and widespread issue of academic integrity transgressions within higher education, reaffirming its status as an epidemic concern.
Streptococcus anginosus: A new pathogen of superficial gastritis, atrophic gastritis and gastric cancer
Authors: Guo Fengting , Li Lanfang , Li Lifang
Published: July 2024

A wealth of research indicates that superficial gastritis (SG) and atrophic gastritis (AG) are precursors to gastric cancer (GC). While Helicobacter pylori (H. pylori) has long been recognized as a key player in GC development, recent findings by Fu et al. have identified Streptococcus anginosus (S. anginosus) as an emerging pathogen that can trigger SG, AG and GC. S. anginosus, a gram-positive coccus, leverages its surface protein T. pallidum membrane protein C (TMPC) to engage with the annexin A2 (ANXA2) receptor of gastric epithelial cells, facilitating its colonization and invasion in the gastric mucosa. This leads to an upregulation of proinflammatory chemokines Ccl20 and Ccl8, causing prolonged effects on gastric barrier function and microbiota homeostasis, leading to SG. Moreover, these bacteria activate the mitogen-activated protein kinase (MAPK) signaling pathway, which is associated with the development of AG and GC. Importantly, inhibiting TMPC or knocking down ANXA2 can reduce S. anginosus colonization and invasion, lowering the chances of SG, AG, and GC. This paper highlights the molecular mechanisms of S. anginosus in SG, AG and GC, emphasizing the importance of a multi-pathogen strategy in gastric disease management and the need for further investigation into the role of S. anginosus in GC progression.
Planar microfluid concentrators based on silagerm 8040 for sampling and sample preparation for the analy-sis of gas media
Authors: Margaryan Asthik E. , Platonov Igor A. , Kolesnichenko Irina N. , Novikova Ekaterina A. , Karsunkina Alesya S.
Published: July 2024

Exhaled air is a matrix with a complex molecular composition, including more than 3,500 components of various origins, the content of which can indicate the normal or pathological state of human health.
One of the selective markers of diabetes formed in exhaled air is acetone. Its increased content (more than2.54 mg/m3) in exhaled air indicates excessive levels of glucose in the blood. To carry out a diagnostically reliable quantitative analysis of acetone in exhaled air, it is necessary to minimize random errors at all stages of sampling, concentration and calibration. The proposed MFC allow to carry out sample preparation and calibration under identical conditions, while combining the stages of sampling and concentration.
The sample was concentrated using microfluidic systems based on Silagerm 8040 filled with a sorbent. Porapak-Q was chosen as the sorbent, which was pre-treated with ethyl alcohol vapour before filling into the channels.
Sorption concentration in dynamic mode using an MFC was carried out by passing the resulting model gas mixture “acetone in air” with a concentration of 2.54 mg/m3 at t = 0oC until breakthrough appears.
Desorption using the MFC based on Porapak-Q was carried out at temperatures of 50°C, 60°C, 70°C in dynamic mode by passing purified air at a rate of 0.5 ml/sec (desorption time is one second). The effluent was analysed by gas chromatography. The main advantage of this system is the ability to include it in a gas microchromatograph. The resulting analytical complex is mobile, which allows the usage for non-invasive diagnostics in non-laboratory conditions. Optimal concentration conditions using the MFC filled with Porapak-Q sorbent at which the maximum concentration coefficient of 43 was achieved have been established (tsorb=0oC; tdes=70oC, Vsorb=45 ml, tdes=1 sec).
In a comparative analysis of the standard sampling method (using a Tedlar bag) and the method proposed using the MFC, it was found that the use of Tedlar bags for sampling exhaled air resulted in significant decrease in the accuracy characteristics (more than 30-65%) within 12 h, which is not applicable for diagnostic purposes. This fact was due to the sorption of the analyte on the walls of the bag and to eliminate undesirable effects it is necessary to use an additional step of sample drying. When the MFC was used for sampling, such a tendency was not observed, the accuracy characteristic did not decrease by more than 6-10% within 8 hours and no additional stages of drying the sample of exhaled air were required.
Physico-chemical and sorption properties of original and modified clay from the Levashinskoye deposit of the Republic of Dagestan
Authors: Sveshnikova Dzhannet A. , Rabadanova Dzhennet I. , Ataev David R. , Ramazanov Arsen Sh.
Published: July 2024

The high sorption, ion exchange, and catalytic properties of natural clay minerals determine their widespread use in various technological processes. More than 30 deposits of various clays have been identified and explored within the territory of the Republic of Dagestan, one of which is the Levashinskoye deposit. Available data on the adsorption of ions of rare alkali (Rb+ and Cs+) and alkaline earth (Sr2+) metals on clay minerals make the task of study of the possibility of using clay from this deposit as a sorbent for extracting these ions from aqueous solutions even more relevant. This, in turn, is due to the need for effective sorbents for concentrating and extracting ions of alkali and alkaline earth metals when solving problems of complex processing of hydromineral raw materials. The purpose of this study was the investigation of the physicochemical and sorption properties of Rb+, Cs+, Sr2+ native and hydrochloric acid-modified clay from the Levashinskoye deposit of the Republic of Dagestan. Based on a comprehensive study, the mineralogical composition of native clay was established, the main clay minerals of which were: illite, nontronite, and montmorillonite. In addition, the sample contained calcite and quartz.
In order to increase the content of clay minerals, with which the sorption activity of clay is mainly associated, the original clay was treated with a 1 M hydrochloric acid solution, which led to an increase in the content of clay minerals from 62% in the original clay to 75% in the clay treated with HCl.
Sorption experiments for Rb+, Cs+, Sr2+ were carried out in model solutions, the concentration of the studied ions in solutions varied in the range of 20-700 mg/dm3. It was shown that the equilibrium of adsorption of these ions on the studied clay can be satisfactorily described both by equations for a non-uniform surface (Freundlich, Temkin, Dubinin-Radushkevich) and by the Langmuir isotherm for a homogeneous surface. It was found that the modification of the original clay with a solution of hydrochloric acid increases the sorption capacity of the clay for the studied ions. It has been established that the main mechanism of sorption of these ions on both the original and modified clay of the Levashinskoye deposit was ion exchange, while other mechanisms cannot be excluded. The study of the kinetics of Rb+, Cs+, Sr2+ sorption on modified clay showed that the sorption process of these ions can be described by a mixed-diffusion model and a pseudo-second-order model.
Transcranial Direct Current Stimulation (tDCS) on Soccer Players: A Mini-Review
Authors: Stefano Pallanti , Carlotta Colzi
Published: July 2024

Background: This mini-review explores the application of Transcranial Direct Current Stimulation (tDCS) in enhancing cognitive and motor performance in soccer players. This review synthesizes findings from recent studies focusing on tDCS’s impact on the primary Motor Cortex (M1) and the Dorsolateral Prefrontal Cortex (dlPFC). Results: studies conducted to date show that tDCS can enhance muscle strength, reduce perceived fatigue, and improve well-being in soccer players. For instance, anodal tDCS applied to the primary motor cortex has been shown to temporarily increase quadriceps strength, while stimulation of the dlPFC has improved recovery post-match and enhanced cognitive functions like reaction time and implicit motor learning. Conclusion: the mini-review highlights the need for more targeted research, emphasizing the importance of individualized protocols and advanced neuroimaging techniques to better understand tDCS’s mechanisms and optimize its use in sports. Future directions suggest adopting neurocircuit-based strategies such as RDoC to tailor interventions more precisely to athletes’ needs. This integration could potentially maximize the benefits of tDCS, offering a holistic approach to enhancing athletic performance and recovery in soccer players.
Model Independent and Dependent Methods for Dissolution Profiles Comparison: Can we take the Road Less Travelled?
Authors: Adithya Karthik Bhattiprolu , Sivacharan Kollipara , Rajkumar Boddu , Arvind Rachapally , Tausif Ahmed
Published: July 2024

Dissolution testing plays an important role in pharmaceutical development due to its ability to provide critical insight into in vivo performance. Dissolution testing during routine pharmaceutical development can ensure batch-to-batch consistency and helps to ensure that the commercial batches are of representative quality to that of studied in the pivotal clinical studies. For comparing the dissolution profiles, there are multitude of approaches suggested by various regulatory agencies that consist of model independent and dependent approaches. The present review aims to summarize the current understanding of dissolution profile similarity approaches. Dissolution profile comparison approaches suggested by various regulatory agencies were compared and contrasted. Further, detailed procedural aspects were provided for the determination of similarity using model independent and dependent approaches. Model independent approaches such as f2 bootstrap, and Multivariate Statistical Distance (MSD) were portrayed from a practical perspective. Advanced tools such as Bias-Corrected and accelerated (BCa) for f2 bootstrap were discussed in depth. Further, model dependent approaches such as zero order, and Weibull models were discussed from realistic scenarios. Finally, the utility of modeling and simulation approaches such as physiologically based pharmacokinetics model (PBPK) and physiologically based biopharmaceutics model (PBBM) were discussed in the context of their ability and potential to supersede traditional dissolution dissimilarity. Overall, this article acts as a ready-to-use guide for pharmaceutics and biopharmaceutics scientists for effective methodologies for dissolution profile comparison for internal decision-making and regulatory justifications.
Coimmunoprecipitation v1
Authors: Wang Shiyi
Published: July 2024

Co-Immunoprecipitation assays in HEK cells
Multicolor fluorescence in situ hybridization and analysis v1
Authors: Chen Chuyu
Published: July 2024

Antipsychotics are known to induce the expression of several immediate early genes (IEGs) via D2R antagonism, which then leads to changes in critical signaling pathways in iSPNs. To define the impact of LRRK2 kinase activity on the expression pattern ofIEGs in the iSPNs, we used multicolored single-molecule fluorescence in situ hybridization.
Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography v1
Authors: Domart Florelle
Published: July 2024

Primary sandwich hippocampal neuron cultures are adapted from the Kaech and Banker protocol (Kaech and Banker, 2006) and provide neuronal cultures with almost no glial cells, which may facilitate the targeting of neurons for cryo-electron tomography (see accompanying protocol by Siegert, Petrovic, Do et al.). In addition, the cells can be seeded at a very low density.
Primary cortical neuron isolation and culture v1
Authors: Wang Shiyi
Published: July 2024

Primary cortical neuron isolation and culture
In vivo BioID protein purification v1
Authors: Wang Shiyi
Published: July 2024

In vivo BioID protein purification
Rat astrocyte isolation and culture v1
Authors: Wang Shiyi
Published: July 2024

Rat astrocyte isolation and culture
Mouse brain slice electrophysiology v1
Authors: Wang Shiyi
Published: July 2024

Mouse brain slice electrophysiology
Protein extraction and Western blotting v1
Authors: Wang Shiyi
Published: July 2024

Protein extraction and Western blotting
shRNA plasmids v1
Authors: Wang Shiyi
Published: July 2024

How to make shRNA plasmids
Reflections on Peter Nagel’s article
Authors: Jones Chris
Published: July 2024

This is a response to an article written by South African New Testament scholar, Peter Nagel. The article draws on the thoughts of another South African New Testament scholar Andries van Aarde and of German theologian Bernhard Lohse, on the history of the doctrine of the Trinity. Concluding observations include inquiring as to how the church will effectively address this type of study in its deliberations and decisions.