bluuebunny/crossref_metadata_2025 huggingface dataset:
3D Differential Equation Model for Patients' Choice of Hospital in China
Authors: Zhao Xiaoxia , Jiang Lihong , Zhao Kaihong
Published: April 2022

The number of patients in a hospital is a direct indicator of patients' choice of hospital, which is a complex process affected by many factors. Based on the national medical system and patients' preference for high-grade hospitals in China, this study establishes a three-dimensional differential equation model for calculating the time variation of the number of visits to three grades of hospitals. We performed a qualitative analysis of the system. We carried out a subsequent numerical simulation to analyze the impact on the system when the rate of leapfrog treatment and the maximum capacity of doctors and treatments changed. The results show that the sustainability of China's three levels of hospitals mainly depends on the level of hospital development. The strength of comprehensive health improvement at specific levels is the key to increasing the service efficiency of medical resources.
The Effectiveness of the Anteroom (Vestibule) Area on Hospital Infection Control and Health Staff Safety: A Systematic Review
Authors: Andalib Elham , Faghani Masoumeh , Zia Ziabari Seyyed Mahdi , Shenagari Mohammad , Salehiniya Hamid , Keivanlou Mohammad Hossein , Rafat Zahra
Published: April 2022

The emergence of SARS-CoV2 in 2019 showed again that the world's healthcare system is not fully equipped and well-designed for preventing the transmission of nosocomial respiratory infections. One of the great tools for preventing the spread of infectious organisms in hospitals is the anteroom. Several articles have investigated the role of the anteroom in disease control but the lack of a comprehensive study in this field prompted us to provide more in-depth information to fill this gap. Also, this study aimed to assess the necessity to construct an anteroom area for hospital staff members at the entrance of each ward of the hospital, and specify the equipment and facilities which make the anteroom more efficient. Articles were identified through searches of Scopus, Web of Sciences, PubMed, and Embase for studies published in English until May 2020 reporting data on the effect of the anteroom (vestibule) area in controlling hospital infections. Data from eligible articles were extracted and presented according to PRISMA's evidence-based data evaluation search strategy. Also, details around the review aims and methods were registered with the PROSPERO. From the database, 209 articles were identified, of which 25 studies met the study criteria. Most studies demonstrated that an anteroom significantly enhances practical system efficiency. The results showed that the equipment such as ventilation system, high-efficiency particulate absorption filter, hand dispensers, alcohol-based disinfection, sink, mirror, transparent panel, UVC disinfection, and zone for PPE change, and parameters like temperature, door type, pressure, and size of the anteroom are factors that are effective on the safety of the hospital environment. Studies demonstrated that providing an anteroom for changing clothing and storing equipment may be useful in reducing the transmission of airborne infections in hospitals. Since the transmission route of SARS-CoV2 is common with other respiratory infectious agents, it can be concluded that a well-designed anteroom could potentially decrease the risk of SARS-CoV2 transmission during hospitalization as well.
Latina Women in Academia: Challenges and Opportunities
Authors: Abraído-Lanza Ana F. , Echeverria Sandra E. , Flórez Karen R. , Mendoza-Grey Sonia
Published: April 2022

Latina women and other ethnic and racial groups continue to be underrepresented in science, technology, engineering, and mathematics (STEM) fields, including public health. This underrepresentation of people from diverse backgrounds and lived experiences in academic public health and other scientific disciplines is a form of epistemic oppression, exclusion that hinders contribution to knowledge production and advancement. Our analysis of 2021 data from the Association of Schools and Programs of Public Health indicates that Latinos/as represented only 6.0% of all instructional faculty and 6.1% of all tenured faculty at schools and programs of public health. We discuss the ways in which sociopolitical contexts, family-level dynamics and gendered norms, and institutional contexts hamper Latinas' full participation in academia. We propose solutions such as redefining metrics for success, leadership accountability, equity analyses, cluster hiring initiatives, and instituting structured mentoring and leadership programs. Bold actions are needed if we are to advance the scientific enterprise and address the diversity and equity problem in public health.
Image Quality Control in Lumbar Spine Radiography Using Enhanced U-Net Neural Networks
Authors: Chen Xiao , Deng Qingshan , Wang Qiang , Liu Xinmiao , Chen Lei , Liu Jinjin , Li Shuangquan , Wang Meihao , Cao Guoquan
Published: April 2022

Purpose

To standardize the radiography imaging procedure, an image quality control framework using the deep learning technique was developed to segment and evaluate lumbar spine x-ray images according to a defined quality control standard.

Materials and Methods

A dataset comprising anteroposterior, lateral, and oblique position lumbar spine x-ray images from 1,389 patients was analyzed in this study. The training set consisted of digital radiography images of 1,070 patients (800, 798, and 623 images of the anteroposterior, lateral, and oblique position, respectively) and the validation set included 319 patients (200, 205, and 156 images of the anteroposterior, lateral, and oblique position, respectively). The quality control standard for lumbar spine x-ray radiography in this study was defined using textbook guidelines of as a reference. An enhanced encoder-decoder fully convolutional network with U-net as the backbone was implemented to segment the anatomical structures in the x-ray images. The segmentations were used to build an automatic assessment method to detect unqualified images. The dice similarity coefficient was used to evaluate segmentation performance.

Results

The dice similarity coefficient of the anteroposterior position images ranged from 0.82 to 0.96 (mean 0.91 ± 0.06); the dice similarity coefficient of the lateral position images ranged from 0.71 to 0.95 (mean 0.87 ± 0.10); the dice similarity coefficient of the oblique position images ranged from 0.66 to 0.93 (mean 0.80 ± 0.14). The accuracy, sensitivity, and specificity of the assessment method on the validation set were 0.971–0.990 (mean 0.98 ± 0.10), 0.714–0.933 (mean 0.86 ± 0.13), and 0.995–1.000 (mean 0.99 ± 0.12) for the three positions, respectively.

Conclusion

This deep learning-based algorithm achieves accurate segmentation of lumbar spine x-ray images. It provides a reliable and efficient method to identify the shape of the lumbar spine while automatically determining the radiographic image quality.
Oral Complications in Cancer Patients–Medication-Related Osteonecrosis of the Jaw (MRONJ)
Authors: Migliorati Cesar Augusto
Published: April 2022

Medication-Related Osteonecrosis of the Jaw (MRONJ) was first reported in 2003. Despite the progress in the understanding of this oral complication in cancer patients for the past 18 years, there is still discussion about the best way to define MRONJ, prevent the complication, how to diagnose, and the options of treatment available. The initial reports associated MRONJ to bisphosphonates and denosumab, medications that work as bone-modifying agents. Later, other agents such as the antiangiogenics, have also been reported to cause the oral complication, either alone or in combination with antiresorptives. Initially, these medications were prescribed to patients with osteoporosis and cancers patients with bone metastasis. Today, because of the effect of the medications in the bone remodeling system, patients with several other diseases such as giant cell tumors, rheumatoid arthritis, Paget's disease of bone, fibrous dysplasia, osteogenesis imperfecta, are managed with these medications, significantly increasing the population of individuals at risk for developing MRONJ. This mini review focused on the cancer patient. It updates the dental clinician on the recent scientific literature about MRONJ and provides information on how to diagnose and manage patients being treated with these medications, suggests protocols to prevent the development of MRONJ, and present ways to manage those patients who develop the oral complication.
Can Bacteria Save an Estuary’s Food Web?
Authors: Tung Alice , Lehman Peggy W. , Durand John
Published: April 2022

Scientists in the San Francisco Estuary (Estuary) are trying to solve the puzzle of why many small native fishes are starving. Zooplankton are important food for small fish in the Estuary, but there are fewer zooplankton in the Estuary than there used to be. Why are zooplankton in such short supply? Possibly because they are hungry, too! Floating plant-like organisms called phytoplankton, the usual food for zooplankton, have disappeared from some areas of the Estuary. However, bacteria may be able to help with this food shortage. Bacteria in the water eat a carbon-containing substance called organic matter that is released from both living and dead organisms. As bacteria eat and grow, they stick together to make clumps of food big enough for zooplankton to eat. Since a lot of organic matter exists in the Estuary, these tiny bacteria may be able to save small native fishes by making food for zooplankton. In this article we will discuss what dissolved organic matter is and how bacteria may be an important puzzle piece for making fish food in the estuary.
Case Report: Posterolateral Epidural Supra-C2-Root Approach (PESCA) for Biopsy of a Retro-Odontoid Lesions in Same Sitting After Occipitocervical Fixation and Decompression in a Case of Crowned Dens Syndrome With Brainstem Compression and Displacement
Authors: Haas Patrick , Hauser Till-Karsten , Kandilaris Kosmas , Skardelly Marco , Tatagiba Marcos , Adib Sasan Darius
Published: April 2022

Background

‘Crowned dens syndrome' (CDS) is a special form of calcium pyrophosphate dihydrate deposition disease which is characterized radiologically by a halo-like or crown-like distribution in the periodontoid region and clinically by cervical pain. Herein, we will describe our experience of posterolateral epidural supra-C2-root approach (PESCA) for biopsy of retro-odontoid lesions in one surgical session after occipitocervical fixation and decompression in a patient with CDS and massive brainstem compression.

Case Presentation

A 70-year-old woman presented to our department with a 4-week history of progressive walking impairment, neck pain, neck rigidity, fever, dizziness, slight palsy of the left hand, and multiple fall episodes. Magnetic resonance imaging (MRI) of the craniovertebral junction (CVJ) and cervical spine revealed a lesion of the odontoid process and the retro-odontoid region with mainly solid components, as well as small cystic components, and brainstem compression and displacement. In first step, fusion surgery of the CVJ C0–C4 was performed with occiptocervical decompression. After fusion and decompression the lower lateral part of the C1 arc and the lateral superior part of the left side of the C2 arc were removed. The entry point was located directly above the superior part of the C2 root. A biopsy of the lateral portions of the lesions was obtained by bioptic forceps under microscope guidance. Pathologic examination of the mass revealed deposition of birefringent crystals compatible with calcium pyrophosphate. In addition to the clinical symptoms (especially neck pain), the diagnosis of CDS was made. Non-steroidal inflammatory drugs (NSAIDs) and colchicine (and later magnesium) were started. At follow-up examination 6 months after surgery, an MRI scan of the cervical spine revealed regression of the pannus and the cyst with replacement of the brainstem, clinical improvement of walking, and increased strength of the left hand.

Conclusions

This study demonstrates that PESCA can be used to obtain tissue for pathological analysis in one surgical sitting after fusion and decompression and that fusion, decompression, and PESCA (in the same session) together with subsequent conservative management could be a good alternative for the treatment of CDS.
The Prognostic Value of Sarcopenia and Myosteatosis in Biliary Tract Cancer After Palliative Treatment With Radiation-Emitting Metallic Stent
Authors: Chen Qi , Lu Jian , Lu Xun , Yao Xi-Juan , Zhang Xuan-Pu , Wang Shang-Yuan , Guo Jin-He
Published: April 2022

Background:

Radiation-emitting metallic stent (REMS) placement is increasingly used for malignant biliary obstruction (MBO) caused by unresectable biliary tract carcinoma (UBTC) in clinical practice. The study is aimed to evaluate the prognostic value of sarcopenia, myosteatosis, and their combination on overall survival (OS) in patients treated with REMS for UBTC.

Methods

Patients diagnosed with UBTC who underwent REMS placement between January 2013 and May 2021 were included consecutively in this retrospective study. Sarcopenia and myosteatosis were defined based on skeletal muscle index (SMI) and skeletal muscle attenuation (SMA), respectively, which were measured by computer tomography (CT) images on the level of the third lumbar vertebral body before REMS placement. Patients were categorized into two groups by sex-specific cutoff value for sarcopenia and myosteatosis, and OS rates were compared between the groups. Univariate and multivariate cox regression analyses were used to assess factors associated with OS.

Results

Data of 135 patients included were retrospectively reviewed and analyzed. Median OS was 7.17 months in total cohort. Patients in the sarcopenia group had significant poorer OS than those in the non-sarcopenia group (median: 3.23 vs. 11.60 months, p < 0.001). OS was shorter in patients with myosteatosis than those without myosteatosis (median: 4.40 vs. 9.17 months, p < 0.001). Sarcopenia (odds ratio [OR] = 9.61; 95% CI = 5.41–17.09; p < 0.001) and myosteatosis (OR = 1.70; 95% CI = 1.13–2.57; p = 0.012) were significantly associated with OS. Combining sarcopenia and myosteatosis (CSM) showed a better predictive accuracy in OS than either one (area under curves: CSM vs. sarcopenia = 0.760 vs. 0.698, p = 0.049; CSM vs. myosteatosis = 0.760 vs. 0.671, p = 0.006).

Conclusion

Sarcopenia and myosteatosis are negative predictors of survival in patients who underwent REMS placement for UBTC. CSM seemed to show a better prognostic value than either sarcopenia or myosteatosis alone. They can be used preoperatively for risk evaluation.
Endocardial Radiofrequency Ablation vs. Septal Myectomy in Patients With Hypertrophic Obstructive Cardiomyopathy: A Systematic Review and Meta-Analysis
Authors: Jiang Tao , Huang Bingyu , Huo Shengqi , Mageta Lulu Monica , Guo Junyi , Lv Jiagao , Lin Li
Published: April 2022

Background

Septal myectomy (SM) has been the gold standard therapy for most patients with hypertrophic obstructive cardiomyopathy (HOCM). Endocardial radiofrequency ablation of septal hypertrophy (ERASH) is a novel treatment for septal reduction. We aimed to assess the efficacy and safety between two treatment strategies.

Methods

We searched PubMed, Web of Science, Cochrane Library, and Embase databases to identify relevant studies published up to March 2021. Random-effect models were used to calculate standardized mean difference (SMD) and 95% confidence intervals (CIs) for resting left ventricular outflow tract gradient (LVOTG) and septal thickness.

Results

Twenty-five studies are included in this review, eighteen studies for SM and seven studies for ERASH. During follow-up, there were significant reductions of the mean resting LVOTG in adults (SM groups: SMD = −3.03, 95% CI [−3.62 to −2.44]; ERASH groups: SMD = −1.95, 95% CI [−2.45 to −1.45]) and children (SM groups: SMD = −2.67, 95% CI [−3.21 to −2.12]; ERASH groups: SMD= −2.37, 95% CI [−3.02 to −1.73]) after the septal reduction therapies. For adults, SM groups contributed to more obvious reduction than ERASH groups in interventricular septal thickness (SM groups: SMD = −1.82, 95% CI [−2.29 to −1.34]; ERASH groups: SMD = −0.43, 95% CI [−1.00 to 0.13]). The improvement of the New York Heart Association class was similar in the two groups (SM groups: 46.4%; ERASH groups: 46.7%). The periprocedural mortality in SM and ERASH were 1.1 and 1.8%, respectively.

Conclusions

This systematic review suggests that SM is superior to ERASH in the treatment of HOCM. But for the patients who are at risk for open cardiac surgeries or prefer a less invasive approach, ERASH might be an optional approach.
Emergence of a Novel Recombinant Pseudorabies Virus Derived From the Field Virus and Its Attenuated Vaccine in China
Authors: Tan Lei , Yao Jun , Lei Lei , Xu Kaiwen , Liao Fan , Yang Shibiao , Yang Lincheng , Shu Xianghua , Duan Deyong , Wang Aibing
Published: April 2022

The occurrence of pseudorabies (PR) caused by the PR virus (PRV) causes huge economic losses to the pig industry in China. Moreover, the potential threat of PRV to humans' health has received wide attention recently. The prevalence of two PRV genotypes and the application of their corresponding live attenuated vaccines increase the recombination possibility. In the present study, a novel recombinant PRV strain designed as HN-2019 was isolated from one sick piglet in Hunan province, China, its genetic features and pathogenicity were further investigated. The results showed that the glycoprotein E (gE) and gG genes of the HN-2019 strain displayed higher nucleotide homology with PRV classical strains (such as Ea and Fa) compared to others. However, its TK gene with continuous nucleotide deletions shared 100% nucleotide identity with the HB-98 vaccine strain, which was derived from the Ea strain. Moreover, the HN-2019 strain exhibited similar growth characteristics to that of the Ea strain, but its pathogenicity in mice was significantly lower than the latter one. The results above suggested that a naturally recombinant event might occur in the genome of the HN-2019 strain between the PRV classical strain and the HB-98 vaccine strain, which will provide useful guidelines for PRV vaccine design in the future.
Bibliometric Analysis of Gig Economy
Authors: Batmunkh Altanshagai , Fekete-Farkas Maria , Lakner Zoltan
Published: April 2022

Technological advances such as smartphones, mobile applications, and online platforms have enabled a new form of economy, known as a gig economy, at a large scale, in which there is a free-market system allowing organizations (job providers) to hire independent contractors (job seeker). Unlike traditional employer and employee relationships, the gig economy creates opportunities for independent workers to seek short-term contract jobs and temporary positions. This article presents a systematic review of the literature associated with a bibliometric analysis of the global perspective of the gig economy. The study aims to present the analysis of published articles that explore the gig economy. Initially, 2297 documents were retrieved by gig economy as a keyword from Google Scholar, Scopus, and Web of Science between 2014 and 2022. After applying certain criteria, only 686 publications were selected for bibliometrics analysis. The selected articles were used to measure bibliometric indicators and evaluate the research work on the gig economy. Bibliometrics an R package for bibliometric and co-citation analysis was used to achieve the results. VOSviewer was also used to analyze the co-occurrence of the keywords. The results highlight the publication trends, top contributing authors and their countries, most cited articles, keywords, and most contributing journals to the research field.
Fault Detection of Aero-Engine Sensor Based on Inception-CNN
Authors: Du Xiao , Chen Jiajie , Zhang Haibo , Wang Jiqiang
Published: April 2022

The aero-engine system is complex, and the working environment is harsh. As the fundamental component of the aero-engine control system, the sensor must monitor its health status. Traditional sensor fault detection algorithms often have many parameters, complex architecture, and low detection accuracy. Aiming at this problem, a convolutional neural network (CNN) whose basic unit is an inception block composed of convolution kernels of different sizes in parallel is proposed. The network fully extracts redundant analytical information between sensors through different size convolution kernels and uses it for aero-engine sensor fault detection. On the sensor failure dataset generated by the Monte Carlo simulation method, the detection accuracy of Inception-CNN is 95.41%, which improves the prediction accuracy by 17.27% and 12.69% compared with the best-performing non-neural network algorithm and simple BP neural networks tested in the paper, respectively. In addition, the method simplifies the traditional fault detection unit composed of multiple fusion algorithms into one detection algorithm, which reduces the complexity of the algorithm. Finally, the effectiveness and feasibility of the method are verified in two aspects of the typical sensor fault detection effect and fault detection and isolation process.
Active Fault-Tolerant Control for Near-Space Hypersonic Vehicles
Authors: Zhao Kai , Song Jia , Ai Shaojie , Xu Xiaowei , Liu Yang
Published: April 2022

Due to the harsh working environment, Near-Space Hypersonic Vehicles (NSHVs) have the characteristics of frequent faults, which seriously affect flight safety. However, most researches focus on active fault-tolerant control for actuator faults. In order to fill the gap of active fault-tolerant control for sensor faults, this paper presents an Active Fault-Tolerant Control (AFTC) strategy for NSHVs based on Active Disturbance Rejection Control (ADRC) combined with fault diagnosis and evaluation. With the proposed AFTC strategy, both sensor faults and actuator faults can be compensated within 0.5 s. Wavelet packet decomposition and Kernel Extreme Learning Machine (KELM) are associated to ensure the high accuracy and real-time ability of fault diagnosis. Simulation results show that the proposed fault diagnosis method can significantly reduce the divergence of diagnosis results by up to 98%. The fault information is used to generate tolerant compensation, which is combined with the ADRC to achieve AFTC. Statistical results indicate that AFTC has significantly lower static error than ADRC. The proposed AFTC method endows NSHVs with the ability to complete missions even when various types of faults appear. Its advantages are demonstrated in comparison with other fault diagnosis and tolerant control methods.
Systematic Reliability-Based Multidisciplinary Optimization by Parallel Adaptive Importance Candidate Region
Authors: Zhang Mengchuang , Xia Shasha , Li Xiaochuan , Yao Qin , Xu Yang , Yin Zhiping
Published: April 2022

Reliability-based design optimization (RBDO) has become a prevalent design for aeronautical and aerospace engineering. The main problem is that it is impractical in complex cases with multi-failure regions, especially in multi-objective optimization. The active learning method can obtain an adaptive size of samples to get a relatively acceptable accuracy. The problem of RBDO using the traditional active learning Kriging (ALK) method is that the design space is generally still and only one training point is selected, which is not reasonable based on the concept of importance sampling and parallel calculation. As a consequence, the accuracy improvement is limited. In this paper, we investigate the method of obtaining an optimal size of design and reliability to assess space in parallel, simultaneously. A strategy of parallel adaptive candidate (PAIC) region with ALK is proposed and a sequential optimization and reliability assessment (SORA) method is modified to efficiently improve the accuracy. Importance sampling is used as a demonstration for the modified SORA with more accuracy. The method is then verified using mathematical cases and a scooping system of an amphibious aircraft.
An Efficient Blade Design Method of a Ducted Fan Coupled with the CFD Modification
Authors: Guo Jiahao , Zhou Zhou
Published: April 2022

In order to improve the design accuracy of the blade design method of a ducted fan based on the blade element momentum theory, the design was modified by the CFD calculation with higher accuracy to propose an integrated and efficient design method for the rotor and stator. Firstly, a fast blade design method for the ducted fan was established based on the blade element momentum theory, and the initial design of the rotor and stator was carried out. Then, the performance of the designed ducted fan was calculated by the CFD method, and the rotor and stator were modified and redesigned according to the CFD results. Such continuous iterations finally made the design results basically consistent with the CFD results so as to improve the design accuracy. The blade design of different ducted fans shows that the unsteady CFD method based on the sliding mesh has higher accuracy and applicability than the MRF method, and the efficient blade design method established in this paper can meet the thrust demand with a small amount of the CFD modification. The thrust design accuracy of the ducted fan was increased by 11.362%, and the torque design accuracy was increased by 8.141%.
Hybrid Guidance Optimization for Multipulse Glideslope Approach with Bearing-Only Navigation
Authors: Yuan Hao , Li Dongxu , Wang Jie
Published: April 2022

This paper proposes a modified glideslope guidance method that optimizes a hybrid multiobjective of bearing-only navigation error and fuel consumption. The traditional glideslope guidance fixes uniform maneuver intervals and the initial approach velocity as a predetermined value, making this approach inflexible. In this paper, the maneuver intervals and the initial approach velocity were used as optimization variables, and a hybrid cost function was designed. The tradeoff between the two objectives was analyzed with a bearing-only navigation simulation conducted to reveal the navigation performance following different resulting trajectories. The result showed that the optimal scheduled times of maneuvers remained relatively stable under different tradeoff weights, while a strong correlation between the optimal initial approach velocity and the tradeoff weight was revealed. Therefore, when the optimization has to be solved several times online with different tradeoff weights, the initial approach velocity can be the only optimization variable, leaving the scheduled times of maneuvers fixed in the optimal values achieved offline. These findings provide a potential reference for far-approach trajectory design of bearing-only navigation.
Pesticide Use under Public Good Agricultural Practices Standard: A Comparative Study in Thailand
Authors: Amekawa Yuichiro , Bumrungsri Sara , Wayo Kanuengnit , Gebre Girma Gezimu , Hongsibsong Surat
Published: April 2022

The local implementation of Thailand’s public good agricultural practices (GAP) certification standard was investigated by examining its effectiveness in raising farmers’ awareness, lowering pesticide use, and thus improving food safety and quality assurance. A total of 98 certified and 100 uncertified farms were surveyed in two Thai provinces, Chanthaburi and Nakhon Si Thammarat, respectively. Considering durian (Durio zibethinus Murr.) cultivation, the economic size of farming in the former region is relatively larger and production more market-oriented, while in the latter region, the farming scale is smaller. It was observed that knowledge and understanding of GAP is much higher among the certified as compared to the uncertified farmers, although the effectiveness of GAP in lowering pesticide use remains unconfirmed. The comparison between the two regions also suggests that farmers’ pesticide use could be affected by other contextual factors to a greater extent than the GAP. Results from a multivariate linear regression analysis highlighted the critical importance of training on Q-GAP and pesticide use in reducing pesticide use among certified farmers. This suggests that GAP applicant farmers should be encouraged to participate in training on those subjects as an integral part of their Q-GAP application or renewal procedure.
Maximum Entropy Niche-Based Modeling for Predicting the Potential Suitable Habitats of a Traditional Medicinal Plant (Rheum nanum) in Asia under Climate Change Conditions
Authors: Xu Wei , Zhu Shuaimeng , Yang Tianli , Cheng Jimin , Jin Jingwei
Published: April 2022

Rheum nanum, a perennial herb, is a famous traditional Chinese medicinal plant that has great value in modern medicine. In order to determine the potential distribution of R. nanum in Asia, we specifically developed the potential distribution maps for three periods (current, 2050s: 2041–2060, and 2070s: 2061–2080) using MaxEnt and ArcGIS, and these were based on the current and future climate data under two climate scenarios (RCP2.6 and RCP6.0). To predict the potential impacts of global warming, we measured the area of suitable habitats, habitat suitability changes, and habitat core changes. We found that bio16 (i.e., the precipitation of the wettest quarter) and bio1 (i.e., the annual mean temperature) were the most important climate factors that influenced the distribution of R. nanum. The areas of high suitable habitats (HH) and middle suitable habitats (MH) in the current period were 156,284.7 ± 0.99 km2 and 361,875.0 ± 3.61 km2, respectively. The areas of HH and MH in 2070RCP6.0 were 27,309.0 ± 0.35 km2 and 123,750 ± 2.36 km2, respectively. The ranges of 82.0–90.3° E, 43.8–46.5° N were the mostly degraded areas of the 2050s and 2070s, and RCP6.0 had a larger decrease in habitable area than that found in RCP2.6. All the HH cores shifted south, and the shift distance of HH in 2070RCP6.0 was 115.65 km. This study provides a feasible approach for efficiently utilizing low-number occurrences, and presents an important attempt at predicting the potential distribution of species based on a small sample size. This may improve our understanding of the impacts of global warming on plant distribution and could be useful for relevant agricultural decision-making.
Climate Change Impacts on Crop Yield of Winter Wheat (Triticum aestivum) and Maize (Zea mays) and Soil Organic Carbon Stocks in Northern China
Authors: Liu Chuang , Yang Huiyi , Gongadze Kate , Harris Paul , Huang Mingbin , Wu Lianhai
Published: April 2022

Agricultural system models provide an effective tool for forecasting crop productivity and nutrient budgets under future climate change. This study investigates the potential impacts of climate change on crop failure, grain yield and soil organic carbon (SOC) for both winter wheat (Triticum aestivum L.) and maize (Zea mays L.) in northern China, using the SPACSYS model. The model was calibrated and validated with datasets from 20-year long-term experiments (1985–2004) for the Loess plateau, and then used to forecast production (2020–2049) under six sharing social-economic pathway climate scenarios for both wheat and maize crops with irrigation. Results suggested that warmer climatic scenarios might be favourable for reducing the crop failure rate and increasing the grain yield for winter wheat, while the same climatic scenarios were unfavourable for maize production in the region. Furthermore, future SOC stocks in the topsoil layer (0–30 cm) could increase but in the subsoil layer (30–100 cm) could decrease, regardless of the chosen crop.
Coupled Bionic Design of Liquid Fertilizer Deep Application Type Opener Based on Sturgeon Streamline to Enhance Opening Performance in Cold Soils of Northeast China
Authors: Wang Jinwu , Wen Nuan , Liu Ziming , Zhou Wenqi , Tang Han , Wang Qi , Wang Jinfeng
Published: April 2022

Liquid fertilizer has many advantages, such as low production cost and little environmental pollution. Liquid fertilizer open furrow strip application method is widely used in fertilizer application operation. The widely used core-share furrow opener has a high operational resistance, disturbing the soil, hurting the crop roots, causing the liquid fertilizer to volatilize and deteriorating the fertilization effect. In this study, based on the streamline curve of the sturgeon body, we designed several bionic sturgeon liquid fertilizer deep application openers by combining bionics and analyzed the effects of several openers under different operating speeds on open furrow resistances and soil disturbance based on the discrete element method. The mechanism of open furrow resistances reduction and efficient soil backfill of the bionic structure were verified by indoor soil bin tests. The test results show that, compared with the core-share type furrow opener, both open furrow resistances and soil disturbance of the bionic sturgeon liquid fertilizer deep application opener are smaller. This study provides theoretical and practical references for the design of liquid fertilizer deep application openers.
Explainable Deep Learning Study for Leaf Disease Classification
Authors: Wei Kaihua , Chen Bojian , Zhang Jingcheng , Fan Shanhui , Wu Kaihua , Liu Guangyu , Chen Dongmei
Published: April 2022

Explainable artificial intelligence has been extensively studied recently. However, the research of interpretable methods in the agricultural field has not been systematically studied. We studied the interpretability of deep learning models in different agricultural classification tasks based on the fruit leaves dataset. The purpose is to explore whether the classification model is more inclined to extract the appearance characteristics of leaves or the texture characteristics of leaf lesions during the feature extraction process. The dataset was arranged into three experiments with different categories. In each experiment, the VGG, GoogLeNet, and ResNet models were used and the ResNet-attention model was applied with three interpretable methods. The results show that the ResNet model has the highest accuracy rate in the three experiments, which are 99.11%, 99.4%, and 99.89%, respectively. It is also found that the attention module could improve the feature extraction of the model, and clarify the focus of the model in different experiments when extracting features. These results will help agricultural practitioners better apply deep learning models to solve more practical problems.
Delphinidins and Naringenin Chalcone Underlying the Fruit Color Changes during Maturity Stages in Eggplant
Authors: Wang Xing , Luo Shuangxia , Li Qiang , Song Lijun , Zhang Weiwei , Yu Ping , Xuan Shuxin , Wang Yanhua , Zhao Jianjun , Chen Xueping , Shen Shuxing
Published: April 2022

Purple eggplant fruits often turn brown during maturity stages, severely influencing their market value and shelf life. However, the metabolites and genes accounting for the fruit color changes are largely unknown. Here, metabolites and gene expression analyses were used to explore the candidates underlying the differences in fruit color changes between two eggplant genotypes, ‘14-345’ and ‘CGN23829’, with contrasting fruit color changes during fruit development. We found that flavonoids are the most differentially accumulated metabolites between ‘14-345’ and ‘CGN23829’. Higher contents of D3R and D3G are important for keeping the purple color at the maturity stage. Naringenin chalcone likely accounts for the color changes from purple to brown in ‘14-345’. Notably, at the physiological maturity stage, lower expression of early biosynthetic genes (EBGs) involved in the flavonoid biosynthesis pathway is important to keep lower contents of metabolites upstream of anthocyanins, thus leading to more purple coloring. Taken together, the results indicate that delphinidins and naringenin chalcone play important roles in determining eggplant fruit color changes at maturity stages, which is helpful for further dissecting the mechanisms underlying fruit color differences and changes in eggplants.
Impact of Irrigation Levels and Weed Control Treatments on Annual Weeds, Physiological Traits and Productivity of Soybean under Clay Soil Conditions
Authors: Khaffagy Azza E. , Mazrou Yasser S. A. , Morsy Akram R. , El-Mansoury Mona A. M. , El-Tokhy Ahmed I. , Hafez Yaser , Abdelaal Khaled , Khedr Rania A.
Published: April 2022

Water scarcity and weed infestation are major challenges to soybean production. Therefore, two field experiments were conducted in a strip plot design including three replicates in the 2019 and 2020 summer seasons at Sakha Agricultural Research Station, Agricultural Research Centre, Egypt, to investigate the effect of three irrigation levels (I0 (100%), I1 (90%), and I2 (80%)), and six weed control treatments of pendimethalin (T1), oxyfluorfen (T2), bentazon + clethodim (T3), imazethapyr (T4), hand-hoeing twice (T5), and untreated check (T6) on annual weeds, physiological traits, water relations, and yield and its components of soybean cv Giza 111. Results indicated that intermediate irrigation levels lead to the highest yield and economic return, while the herbicide program that resulted in the greatest weed control gave the highest yield. The rate of weed infestation in field experiments was 19.7 and 21.8 t ha−1 dry weights, which decreased soybean yield by 56.9 and 57.1%, compared to treatment (T4). The interactions between 100 irrigation level (I0) and imazethapyr (T4), bentazon + clethodim (T3), and hand-hoeing twice (T5) gave the highest significant values of the most studied characters. Furthermore, under 90% irrigation, all weed control treatments give the best control of weeds and the highest values of the economic criteria.
Oocyte Quality, In Vitro Fertilization and Embryo Development of Alpaca Oocytes Collected by Ultrasound-Guided Follicular Aspiration or from Slaughterhouse Ovaries
Authors: Landeo Leandra , Zuñiga Michele , Gastelu Teddy , Artica Marino , Ruiz Jaime , Silva Mauricio , Ratto Marcelo H.
Published: April 2022

The morphological quality and the in vitro developmental competence of cumulus-oocyte complexes (COCs) collected from in vivo or slaughtered alpacas was compared. COCs were recovered from ovarian follicles using: (i) manual aspiration in ovaries of alpacas (n = 15) sacrificed at a local slaughterhouse, or (ii) transrectal ultrasound-guided follicular aspiration (or ovum-pick-up, OPU) in live alpacas (n = 13) 4 days after the administration of an ovarian superstimulation protocol (200 UI eCG). COCs recovered from both groups were morphologically evaluated and graded. Grade I to III COCs were in vitro matured for 26 h and in vitro fertilized afterwards for 20 h using fresh alpaca epididymal spermatozoa. Presumptive zygotes from both groups were in vitro cultured for 7 days. The proportion of COCs recovered over the total number of follicles punctured was similar between groups, but the mean number of COCs collected from individual ovaries was greater (p < 0.05) in slaughterhouse ovaries. A significantly higher (p < 0.05) percentage of low-quality COCs (grades III and IV) and a lower (p < 0.05) percentage of grade I COCs was obtained using OPU. The number of blastocysts, regarding cleavage and COCs collected, was higher (p < 0.007 and p < 0.0002 respectively) for COCs collected by OPU; however, the total number of blastocysts per female did not differ between groups. We can conclude that the recovery rate and morphological quality of COCs was significantly higher when follicles were manually aspirated from slaughterhouse alpaca ovaries; however, a statistically higher developmental potential was observed in oocytes collected by OPU from live alpaca donors.
A Technical Note of Improvement of the Elnady Technique for Tissue Preservation in Veterinary Anatomy
Authors: Bernal Valentina , Aburto Pedro , Pérez Bárbara , Gómez Marcelo , Gutierrez Juan Claudio
Published: April 2022

Teaching veterinary anatomy has been subjected to changes and restrictions that have promoted the development of new techniques for preserving organs and cadavers. The Elnady technique is a recent method for the conservation of tissues. Specimens produced with this technique are realistic, durable, soft, and flexible, but an undesirable feature is the discoloration of tissues. In the present study, we describe modifications of the Elnady technique for organ and tissue preservation. Specimens were prepared on the theoretical basis of the Elnady technique, but at low temperatures and with longer durations for the fixation, dehydration, glycerin impregnation and curing processes. Furthermore, the tissues were pigmented with a red vegetable pigment before dehydration or in the glycerin impregnation process. The results show high-quality specimens with minimal shrinkage and natural color aspects. The modified Elnady technique is adequate for producing specimens of better contrast for education purposes.
Comparative Analysis of Bacterial Diversity between the Liquid Phase and Adherent Fraction within the Donkey Caeco-Colic Ecosystem
Authors: Zhang Zhenwei , Huang Bingjian , Shi Xiaoyuan , Wang Tianqi , Wang Yonghui , Zhu Mingxia , Wang Changfa
Published: April 2022

Donkey hindgut is an enlarged fermentative chamber that harbors a highly complex and extremely abundant community of anaerobic bacteria. It can be divided into two different ecological sites: liquid (Lq) phase and adherent fraction (Ad) colonized by bacteria. However, the Ad bacteria have not previously been specifically collected or directly compared with the Lq bacteria. In the present study, the digesta collected from the caecum, ventral colon and dorsal colon of nine Dezhou donkeys was separated into Lq and Ad fractions. The bacterial community structure was comparatively determined using 16S rRNA gene sequences by Illumina MiSeq. The Ad bacteria had a higher bacterial diversity than Lq bacteria due to the higher Chao and ACE index (p < 0.05). The predominant bacteria at the phylum level were Firmicutes (55.4~74.3%) and Bacteroidota (13.7~32.2%) for both the Lq and Ad fraction. The relative abundance of Bacteroidota, Spirochaetota, Fibrobacterota and Patescibacteria in the Ad fraction was greater than Lq (p < 0.05), suggesting that bacteria associated with feed particles were mainly responsible for plant fiber degradation. At the genus level, the abundance of Lactobacillus in Lq was greater than that in the Ad fraction (p < 0.05), indicating that the bacteria in the Lq fraction were better at hydrolyzing readily fermentable carbohydrates. PICRUSt showed that the activities of enzymes related to fiber degradation in the Ad fraction were also greater than Lq. In addition, the hindgut region also had a significant effect on the bacterial community composition. The relative abundance of Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, Christensenellaceae_R-7_group and norank_Bacteroidales_BS11_gut_group was increased (p < 0.05) along the donkey hindgut. In summary, the present study provides evidence that bacteria adherent to plant biomass were different to those in the liquid phase within the donkey caeco-colic digesta, and bacteria associated with feed particles may mainly be responsible for plant fiber degradation.
Treatment Outcome in Patients with Mycobacterium abscessus Complex Lung Disease: The Impact of Tigecycline and Amikacin
Authors: Yang Jeng-How , Wang Ping-Huai , Pan Sheng-Wei , Wei Yu-Feng , Chen Chung-Yu , Lee Ho-Sheng , Shu Chin-Chung , Wu Ting-Shu
Published: April 2022

Background: The contemporary guidelines have recommended multiple antimicrobial therapies along with oral macrolides for the treatment of Mycobacterium abscessus complex lung disease (MABC-LD). However, there is little evidence supporting the parenteral tigecycline-containing regimens against MABC-LD. Therefore, we conducted this study to evaluate the effect of intravenous tigecycline-containing regimens on the treatment of MABC-LD. Methods: A retrospective study was conducted in 6 medical centers. Patients with MABC-LD that were followed up at ≥12 months were enrolled. Mycobacterium abscessus subspecies were identified by hsp65, rpoB, secA1 gene PCR, and sequencing. Antimicrobial susceptibility was determined for 34 patients using broth microdilution methods following the Clinical and Laboratory Standards Institute (CLSI) guideline. The microbiology and treatment outcomes were defined as either success or failure. The impacts of tigecycline and amikacin were adjusted for age, comorbidities, surgical resection, and radiologic scores. Results: During the study period, seventy-one patients were enrolled for final analysis. The microbiology failure rate was 61% (43/71) and the treatment failure rate was 62% (44/71). For M. abscessus complex, 97% (33/34) of tigecycline MIC were ≤1 mg/L. Amikacin also demonstrated great susceptibility (94.1%; 32/34). Treatment with regimens containing tigecycline plus amikacin provided better microbiology success (adjusted OR 17.724; 95% CI 1.227–267.206) and treatment success (adjusted OR 14.085; 95% CI 1.103–166.667). Conclusion: The outcome of MABC-LD is always unsatisfactory. Treatment regimens with oral macrolide in combination with tigecycline and amikacin were correlated with increased microbiology success and less treatment failure.
Polymicrobial Biofilm Dynamics of Multidrug-Resistant Candida albicans and Ampicillin-Resistant Escherichia coli and Antimicrobial Inhibition by Aqueous Garlic Extract
Authors: Ashrit Priya , Sadanandan Bindu , Shetty Kalidas , Vaniyamparambath Vijayalakshmi
Published: April 2022

The polymicrobial biofilm of C. albicans with E. coli exhibits a dynamic interspecies interaction and is refractory to conventional antimicrobials. In this study, a high biofilm-forming multidrug-resistant strain of C. albicans overcomes inhibition by E. coli in a 24 h coculture. However, following treatment with whole Aqueous Garlic Extract (AGE), these individual biofilms of multidrug-resistant C. albicans M-207 and Ampicillin-resistant Escherichia coli ATCC 39936 and their polymicrobial biofilm were prevented, as evidenced by biochemical and structural characterization. This study advances the antimicrobial potential of AGE to inhibit drug-resistant C. albicans and bacterial-associated polymicrobial biofilms, suggesting the potential for effective combinatorial and synergistic antimicrobial designs with minimal side effects.
Cefiderocol Protects against Cytokine- and Endotoxin-Induced Disruption of Vascular Endothelial Cell Integrity in an In Vitro Experimental Model
Authors: Hildebrand Dagmar , Böhringer Jana , Körner Eva , Chiriac Ute , Förmer Sandra , Sähr Aline , Hoppe-Tichy Torsten , Heeg Klaus , Nurjadi Dennis
Published: April 2022

The severe course of bloodstream infections with Gram-negative bacilli can lead to organ dysfunctions and compromise the integrity of the vascular barrier, which are the hallmarks of sepsis. This study aimed to investigate the potential effect of cefiderocol on the barrier function of vascular endothelial cells (vECs) in an in vitro experimental set-up. Human umbilical vein cells (HUVECs), co-cultured with erythrocyte-depleted whole blood for up to 48 h, were activated with tumor necrosis factor-alpha (TNF-α) or lipopolysaccharide (LPS) to induce endothelial damage in the absence or presence of cefiderocol (concentrations of 10, 40 and 70 mg/L). The endothelial integrity was quantified using transendothelial electrical resistance (TEER) measurement, performed at 0, 3, 24 and 48 h after stimulation. Stimulation with TNF-α and LPS increased the endothelial permeability assessed by TEER at 24 and 48 h of co-culture. Furthermore, cefiderocol reduces interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α release in peripheral blood mononuclear cells (PBMCs) following LPS stimulation in a dose-dependent manner. Collectively, the data suggest that cefiderocol may have an influence on the cellular immune response and might support the maintenance of vEC integrity during inflammation associated with infection with Gram-negative bacteria, which warrants further investigations.