bluuebunny/crossref_metadata_2025 huggingface dataset:
The marine litter issue in the Windward Islands- a pathway to responses using the DPSIR framework
Authors: Graham Roxanne E. D.
Published: March 2023

Marine litter is an important and worldwide concern that should be deeper analysed. It also requires information from all over the world and especially in less studied areas like small islands developing states, e.g., the Windward Islands of the Eastern Caribbean. This study analyses the issue of marine litter in the English-speaking Windward Islands using the Driver-Pressure-State-Impact-Response (DPSIR) framework. The research is based on a bibliographical review and analysis of scientific and grey literature regarding these islands and the Wider Caribbean Region at large. The Windward Islands present a unique situation because of their geographic location in the south-eastern Caribbean, socio-economic status, and strong external influences. The DPSIR Framework was used to frame and assess the major types, causes and effects of marine litter for the Windward Islands by reviewing current scientific and grey literature. The major solid waste item retrieved from the coastal and marine environment is fishing gear in the case of Dominica and plastic waste for the other islands. The challenges in current and proposed management measures were identified. Proposed solutions in the form of responses were tailored to meet each casual and effect component of the DPSIR framework. The study strongly recommends that the islands make use of their existing participation in global agreements and initiatives, participate more actively in the negotiations for the upcoming Global Plastics Treaty set to launch in 2024, and tackle their mutual need for effective solid waste management systems and infrastructure and increased recycling efforts or opportunities. These measures could undoubtedly pave a way to the achieving a cleaner and healthier ocean.
Air pollution associated with hospital visits for mental and behavioral disorders in Northeast China
Authors: Liu Huo , Zhao Hang , Huang Jinling , He Miao
Published: March 2023

Background

Related studies have found that air pollution is an important factor affecting mental and behavioral disorders. Thus, we performed this time-series study to evaluate the relationship between short-term exposure to ambient air pollutants and visits to hospital by patients with mental and behavioral disorders in northeastern China.

Methods

We used quasi-Poisson regression models and generalized additive models to probe the links between air pollution and mental and behavioral disorders. The possible influences were also explored stratified by season, age and gender.

Results

We found that sulfur dioxide (SO2) had a cumulative effect on mental and behavioral disorders at lag04–lag07 and had the greatest effect at lag07 [Relative risk (RR) = 1.068, 95%CI = 1.021–1.117]. Particulate matter of size 2.5 μm (PM2.5) and SO2 had a cumulative effect on depression and both had the largest effect at lag07 (RR = 1.021, 95%CI = 1.002–1.041; RR = 1.103, 95%CI = 1.032–1.178); SO2 also had a cumulative effect on anxiety disorders, with the largest effect at lag06 (RR = 1.058, 95%CI = 1.009–1.110). In the stratified analysis, people are more susceptible in the cold season compared to the warm season and females and the 18–60-year age group are more sensitive to air pollutants. It is suggested to strengthen management and preventive measures to decrease air pollution exposure.

Conclusion

This study found an association between increased concentrations of air pollutants and increased outpatient visits for mental and behavioral disorders. We recommend that preventive and protective measures should be strengthened in an effort to reduce exposure to air pollution in order to maintain physical and mental health.
Real-life effects of dupilumab in patients with severe type 2 asthma, according to atopic trait and presence of chronic rhinosinusitis with nasal polyps
Authors: Pelaia Corrado , Benfante Alida , Busceti Maria Teresa , Caiaffa Maria Filomena , Campisi Raffaele , Carpagnano Giovanna Elisiana , Crimi Nunzio , D’Amato Maria , Foschino Barbaro Maria Pia , Maglio Angelantonio , Minenna Elena , Nolasco Santi , Paglino Giuseppe , Papia Francesco , Pelaia Girolamo , Portacci Andrea , Ricciardi Luisa , Scichilone Nicola , Scioscia Giulia , Triggiani Massimo , Valenti Giuseppe , Vatrella Alessandro , Crimi Claudia
Published: March 2023

Background

The efficacy of dupilumab as biological treatment of severe asthma and chronic rhinosinusitis with nasal polyps (CRSwNP) depends on its ability to inhibit the pathophysiologic mechanisms involved in type 2 inflammation.

Objective

To assess in a large sample of subjects with severe asthma, the therapeutic impact of dupilumab in real-life, with regard to positive or negative skin prick test (SPT) and CRSwNP presence or absence.

Methods

Clinical, functional, and laboratory parameters were measured at baseline and 24 weeks after the first dupilumab administration. Moreover, a comparative evaluation was carried out in relation to the presence or absence of SPT positivity and CRSwNP.

Results

Among the 127 recruited patients with severe asthma, 90 had positive SPT, while 78 reported CRSwNP. Compared with the 6 months preceding the first dupilumab injection, asthma exacerbations decreased from 4.0 (2.0-5.0) to 0.0 (0.0-0.0) (p < 0.0001), as well as the daily prednisone intake fell from 12.50 mg (0.00-25.00) to 0.00 mg (0.00-0.00) (p < 0.0001). In the same period, asthma control test (ACT) score increased from 14 (10-18) to 22 (20-24) (p < 0.0001), and sino-nasal outcome test (SNOT-22) score dropped from 55.84 ± 20.32 to 19.76 ± 12.76 (p < 0.0001). Moreover, we observed relevant increases in forced expiratory volume in one second (FEV1) from the baseline value of 2.13 L (1.62-2.81) to 2.39 L (1.89-3.06) (p < 0.0001). Fractional exhaled nitric oxide (FeNO) values decreased from 27.0 ppb (18.0-37.5) to 13.0 ppb (5.0-20.0) (p < 0.0001). These improvements were quite similar in subgroups of patients characterized by SPT negativity or positivity, and CRSwNP absence or presence. No statistically significant correlations were detected between serum IgE levels, baseline blood eosinophils or FeNO levels and dupilumab-induced changes, with the exception of FEV1 increase, which was shown to be positively correlated with FeNO values (r = 0.3147; p < 0.01).

Conclusion

Our results consolidate the strategic position of dupilumab in its role as an excellent therapeutic option currently available within the context of modern biological treatments of severe asthma and CRSwNP, frequently driven by type 2 airway inflammation.
Efficacy and safety of sublingual immunotherapy for allergic rhinitis: A network meta-analysis
Authors: Ji Zao , Jiang Feifei
Published: March 2023

Background

To systematically evaluate the clinical efficacy and safety of sublingual immunotherapy for allergic rhinitis (AR) and provide evidence for clinical treatment.

Methods

A literature search was performed on the China National Knowledge Infrastructure (CNKI), Wanfang database, PubMed, Web of Science, Cochrane Library, and Embase database. Data from randomized controlled trials (RCTs) of sublingual immunotherapy for AR were screened and extracted from the establishment of those databases to November 2022. Subsequently, a network meta-analysis was performed using a statistical software R 4.2.

Results

Totally 22 RCTs that met the inclusion and exclusion criteria and screened from 1,164 literature were included. A total of 4,941 AR patients were involved in the 22 trials, as well as five interventions including placebo, pharmacotherapy, subcutaneous immunotherapy_dust mite, sublingual immunotherapy_dust mite, and sublingual immunotherapy_ grass mix plus pollen extract. The results of network meta-analysis showed that, based on symptom scores after different interventions for AR, the most effective treatments for AR were in order as follows: sublingual immunotherapy_dust mite, subcutaneous immunotherapy_dust mite, sublingual immunotherapy_ grass mix plus pollen extract, placebo, and pharmacotherapy. Importantly, sublingual immunotherapy had fewer adverse reactions and higher safety.

Conclusion

Sublingual immunotherapy_dust mite for AR has the best efficacy, whereas traditional medicine has the worst. More high-quality studies with a large sample and multiple centers are needed to verify this conclusion in the future, so as to further provide more reliable evidence-based medical evidence for the clinical treatment options of AR patients.
Vision meets algae: A novel way for microalgae recognization and health monitor
Authors: Zhou Shizheng , Jiang Juntao , Hong Xiaohan , Fu Pengcheng , Yan Hong
Published: March 2023

Marine microalgae are widespread in the ocean and play a crucial role in the ecosystem. Automatic identification and location of marine microalgae in microscopy images would help establish marine ecological environment monitoring and water quality evaluation system. We proposed a new dataset for the detection of marine microalgae and a range of detection methods, the dataset including images of different genus of algae and the same genus in different states. We set the number of unbalanced classes in the data set and added images of mixed water samples in the test set to simulate the actual situation in the field. Then we trained, validated and tested the, TOOD, YOLOv5, YOLOv8 and variants of RCNN algorithms on this dataset. The results showed both one-stage and two-stage object detection models can achieve high mean average precision, which proves the ability of computer vision in multi-object detection of microalgae, and provides basic data and models for real-time detection of microalgal cells.
Submarine groundwater discharge in Dongshan Bay, China: A master regulator of nutrients in spring and potential national significance of small bays
Authors: Sun Yafei , Wang Guizhi , Weng Yubin , Li Qing , Zhang Fei , Jiang Weizhen , Dai Guiyuan , Lin Wen , Sun Shengyao , Jiang Yiyong , Zhang Yuanjing
Published: March 2023

Despite over 90% of China’s coastal bays have an area less than 500 km2, the geochemical effects of SGD on those ecosystems are ambiguous. Based on mapping and time-series observations of Ra isotopes and nutrients, a case study of small bays (<500 km2), we revealed that submarine groundwater discharge (SGD) predominately regulated the distribution of nutrients and fueled algal growth in Dongshan Bay, China. On the bay-wide scale, the SGD rate was estimated to be 0.048 ± 0.022 m day−1 and contributed over 95% of the nutrients. At the time-series site where the bay-wide highest Ra activities in the bottom water marked an SGD hotspot with an average rate an order of magnitude greater, the maximum chlorophyll concentration co-occurred, suggesting that SGD may support the algal bloom. The ever-most significant positive correlations between 228Ra and nutrients throughout the water column (P< 0.01, R2 > 0.90 except for soluble reactive phosphorus in the surface) suggested the predominance of SGD in controlling nutrient distribution in the bay. Extrapolated to a national scale, the SGD-carried dissolved inorganic nitrogen flux in small bays was twice as much as those in large bays (>2,000 km2). Thus, the SGD-carried nutrients in small bays merit immediate attention in environmental monitoring and management.
The effects of ECMO on neurological function recovery of critical patients: A double-edged sword
Authors: Cai Jinxia , Abudou Halidan , Chen Yuansen , Wang Haiwang , Wang Yiping , Li Wenli , Li Duo , Niu Yanxiang , Chen Xin , Liu Yanqing , Li Yongmao , Liu Ziquan , Meng Xiangyan , Fan Haojun
Published: March 2023

Extracorporeal membrane oxygenation (ECMO) played an important role in the treatment of patients with critical care such as cardiac arrest (CA) and acute respiratory distress syndrome. ECMO is gradually showing its advantages in terms of speed and effectiveness of circulatory support, as it provides adequate cerebral blood flow (CBF) to the patient and ensures the perfusion of organs. ECMO enhances patient survival and improves their neurological prognosis. However, ECMO-related brain complications are also important because of the high risk of death and the associated poor outcomes. We summarized the reported complications related to ECMO for patients with CA, such as north–south syndrome, hypoxic–ischemic brain injury, cerebral ischemia–reperfusion injury, impaired intracranial vascular autoregulation, embolic stroke, intracranial hemorrhage, and brain death. The exact mechanism of ECMO on the role of brain function is unclear. Here we review the pathophysiological mechanisms associated with ECMO in the protection of neurologic function in recent years, as well as the ECMO-related complications in brain and the means to improve it, to provide ideas for the treatment of brain function protection in CA patients.
Reducing bias in microbiome research: Comparing methods from sample collection to sequencing
Authors: Kool Jolanda , Tymchenko Liza , Shetty Sudarshan A. , Fuentes Susana
Published: March 2023

Background

Microbiota profiles are strongly influenced by many technical aspects that impact the ability of researchers to compare results. To investigate and identify potential biases introduced by technical variations, we compared several approaches throughout the entire workflow of a microbiome study, from sample collection to sequencing, using commercially available mock communities (from bacterial strains as well as from DNA) and multiple human fecal samples, including a large set of positive controls created as a random mix of several participant samples.

Methods

Human fecal material was sampled, and aliquots were used to test two commercially available stabilization solutions (OMNIgene·GUT and Zymo Research) in comparison to samples frozen immediately upon collection. In addition, the methodology for DNA extraction, input of DNA, or the number of PCR cycles were analyzed. Furthermore, to investigate the potential batch effects in DNA extraction, sequencing, and barcoding, we included 139 positive controls.

Results

Samples preserved in both the stabilization buffers limited the overgrowth of Enterobacteriaceae when compared to unpreserved samples stored at room temperature (RT). These stabilized samples stored at RT were different from immediately frozen samples, where the relative abundance of Bacteroidota was higher and Actinobacteriota and Firmicutes were lower. As reported previously, the method used for cell disruption was a major contributor to variation in microbiota composition. In addition, a high number of cycles during PCR lead to an increase in contaminants detected in the negative controls. The DNA extraction had a significant impact on the microbial composition, also observed with the use of different Illumina barcodes during library preparation and sequencing, while no batch effect was observed in replicate runs.

Conclusion

Our study reaffirms the importance of the mechanical cell disruption method and immediate frozen storage as critical aspects in fecal microbiota studies. A comparison of storage conditions revealed that the bias was limited in RT samples preserved in stabilization systems, and these may be a suitable compromise when logistics are challenging due to the size or location of a study. Moreover, to reduce the effect of contaminants in fecal microbiota profiling studies, we suggest the use of ~125 pg input DNA and 25 PCR cycles as optimal parameters during library preparation.
IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine
Authors: Ma Jiangang , Wang Juan , Yang Hua , Su Mengru , Li Ruichao , Bai Li , Feng Jie , Huang Yuting , Yang Zengqi , Tang Biao
Published: March 2023

The tigecycline resistance gene tet(X4) was widespread in various bacteria. However, limited information about the plasmid harboring the tet(X4) gene spread among the different species is available. Here, we investigated the transmission mechanisms of the tet(X4) gene spread among bacteria in a pig farm. The tet(X4) positive Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Enterobacter hormaeche were identified in the same farm. The whole genome sequencing (WGS) analysis showed that the K. pneumoniae belonged to ST727 (n = 11) and ST3830 (n = 1), E. cloacae and E. hormaeche belonged to ST524 (n = 1) and ST1862 (n = 1). All tet(X4) genes were located on the IncHI1 plasmids that could be conjugatively transferred into the recipient E. coli C600 at 30°C. Moreover, a fusion plasmid was identified that the IncHI1 plasmid recombined with the IncN plasmid mediated by ISCR2 during the conjugation from strains B12L to C600 (pB12L-EC-1). The fusion plasmid also has been discovered in a K. pneumoniae (K1L) that could provide more opportunities to spread antimicrobial resistance genes. The tet(X4) plasmids in these bacteria are derived from the same plasmid with a similar structure. Moreover, all the IncHI1 plasmids harboring the tet(X4) gene in GenBank belonged to the pST17, the newly defined pMLST. The antimicrobial susceptibility testing was performed by broth microdilution method showing the transconjugants acquired the most antimicrobial resistance from the donor strains. Taken together, this report provides evidence that IncHI1/pST17 is an important carrier for the tet(X4) spread in Enterobacteriaceae species, and these transmission mechanisms may perform in the environment.
Rapid diagnosis of duck Tembusu virus and goose astrovirus with TaqMan-based duplex real-time PCR
Authors: Li Haiqin , Wan Chunhe , Wang Zhangzhang , Tan Jia , Tan Meifang , Zeng Yanbing , Huang Jiangnan , Huang Yu , Su Qi , Kang Zhaofeng , Guo Xiaoquan
Published: March 2023

The mixed infection of duck Tembusu virus (DTMUV) and goose astrovirus (GoAstV) is an important problem that endangers the goose industry. Although quantitative PCR has been widely used in monitoring these two viruses, there is no reliable method to detect them at the same time. In this study, by analyzing the published genomes of DTMUV and goose astrovirus genotype 2 (GoAstV-2) isolated in China, we found that both viruses have high conservation, showing 96.5 to 99.5% identities within different strains of DTMUV and GoAstV, respectively. Subsequently, PCR primers and TaqMan probes were designed to identify DTMUV and GoAstV-2, and different fluorescent reporters were given to two probes for differential diagnosis. Through the optimization and verification, this study finally developed a duplex TaqMan qPCR method that can simultaneously detect the above two viruses. The lower limits of detection were 100 copies/μL and 10 copies/μL for DTMUV and GoAstV-2 under optimal condition. The assay was also highly specific in detecting one or two viruses in various combinations in specimens, and provide tool for clinical diagnosis of mixed infections of viruses in goose.
Evaluation of a novel scoring system based on thrombosis and inflammation for predicting stroke-associated pneumonia: A retrospective cohort study
Authors: Li Dongze , Liu Yi , Jia Yu , Yu Jing , Chen Xiaoli , Li Hong , Ye Lei , Wan Zhi , Zeng Zhi , Cao Yu
Published: March 2023

Background

Inflammation and thrombosis are involved in the development of stroke-associated pneumonia (SAP). Our aim was to evaluate the predictive value of a novel, simplified, thrombo-inflammatory prognostic score (TIPS) that combines both inflammatory and thrombus biomarkers in the early phase of ischemic stroke (IS).

Methods

The study population consisted of 897 patients with a first diagnosis of IS admitted to the emergency department of five tertiary hospitals in China. Of these, the data from 70% of patients was randomly selected to derive the model and the other 30% for model validation. A TIPS of “2” was indicative of high inflammation and thrombosis biomarkers and “1” of one biomarker, with “0” indicative of absence of biomarkers. Multivariate logistic regression analyses were used to identify the association between TIPS and SAP.

Results

The TIPS was an independent predictor of SAP and 90-day mortality, with the incidence of SAP being significantly higher for patients with a high TIPS. The TIPS provided superior predictive value for SAP than clinical scores (A2DS2) and biomarkers currently used in practice, for both the derivation and validation sets. Mediation analysis revealed that TIPS provided a predictive value than either thrombotic (NLR) and inflammatory (D-dimer) biomarkers alone.

Conclusion

The TIPS score may be a useful tool for early identification of patients at high-risk for SAP after IS.
Posttranslational modifications of proteins in diseased retina
Authors: Starr Christopher R. , Gorbatyuk Marina S.
Published: March 2023

Posttranslational modifications (PTMs) are known to constitute a key step in protein biosynthesis and in the regulation of protein functions. Recent breakthroughs in protein purification strategies and current proteome technologies make it possible to identify the proteomics of healthy and diseased retinas. Despite these advantages, the research field identifying sets of posttranslationally modified proteins (PTMomes) related to diseased retinas is significantly lagging, despite knowledge of the major retina PTMome being critical to drug development. In this review, we highlight current updates regarding the PTMomes in three retinal degenerative diseases—namely, diabetic retinopathy (DR), glaucoma, and retinitis pigmentosa (RP). A literature search reveals the necessity to expedite investigations into essential PTMomes in the diseased retina and validate their physiological roles. This knowledge would accelerate the development of treatments for retinal degenerative disorders and the prevention of blindness in affected populations.
Aspiration thrombectomy of M2 middle cerebral artery occlusion to treat acute ischemic stroke: A core lab–adjudicated subset analysis from the COMPLETE registry and literature review
Authors: Fifi Johanna T. , Yaeger Kurt , Matsoukas Stavros , Hassan Ameer E. , Yoo Albert , Sheth Sunil , Zaidat Osama O.
Published: March 2023

Background

Although the benefits of aspiration thrombectomy for treating acute ischemic stroke caused by proximal large vessel occlusion have been established, fewer data are available for evaluating aspiration thrombectomy of distal occlusion. The objective of this study was to evaluate, by means of prospectively collected data, the safety and efficacy of aspiration thrombectomy in patients with M2 middle cerebral artery (MCA) occlusion.

Methods

This study is a subset analysis of a global prospective multicenter observational registry that included patients who presented with either anterior or posterior large vessel occlusion and were eligible for mechanical thrombectomy using the Penumbra System including the Penumbra 3D Revascularization Device. For this analysis, all patients in the registry with M2 MCA occlusion were included.

Results

Of the 650 patients in the registry, 113 (17.4%) had M2 MCA occlusion. The rate of a modified treatment in cerebral infarction score of 2b to 3 after the procedure was 79.6% (90/113), the rate of a modified Rankin Scale score of 0–2 at 90 days was 72.5% (79/109), and the all-cause mortality rate at 90 days was 8.8% (10/113). Device-related serious adverse events occurred in one patient (0.9%) within 24 h and in two patients (1.8%) overall. Procedure-related serious adverse events occurred in four patients (3.5%) within 24 h and in six patients (5.3%) overall (nine events).

Conclusion

For appropriately selected patients, aspiration thrombectomy for acute ischemic stroke due to M2 MCA occlusion was safe and effective, with high rates of technical success and good functional outcome.
Electroacupuncture for psychogenic erectile dysfunction: A resting-state functional magnetic resonance imaging study exploring the alteration of fractional amplitude of low frequency fluctuation
Authors: Yang Yuqing , Qu Liu , Mu Linxuan , Yao Junpeng , Su Chengguo , Zheng Qianhua , Zheng Huabin , Zhang Peihai , Li Ying
Published: March 2023

Background

Psychogenic erectile dysfunction (PED) can seriously affect emotional and marital wellbeing. Electroacupuncture (EA) seems an effective method for treating PED. However, the central mechanisms underlying PED and the beneficial effects of EA treatment are unclear. The purpose of this study was to explore the central mechanisms of PED and to examine the impact of EA on erectile function.

Methods

We recruited 14 PED patients and 14 matched normal controls (NCs). PED patients underwent twice rs-fMRI scans, respectively, pre- and post-treatment. The NCs only completed one rs-fMRI scan. We used the fractional amplitude of low frequency fluctuation (fALFF) to compare spontaneous neural activity between the PED patients and NCs, and to examine the differences between the pre- and post-EA treatment scans in the PED patients.

Results

Scores on the IIEF5, QEQ, and SEAR improved after EA treatment. Compared with the NCs, PED patients showed increased fALFF in the right posterior cingulate cortex (PCC), right dorsolateral prefrontal cortex (DLPFC), right supplementary motor area (SMA), and left middle occipital gyrus. Most of these regions are closely implicated in sexual inhibition. The results of the correlation analysis results indicated that the fALFF of the right PCC was negatively correlated with IIEF5 scores. After treatment, fALFF values were substantially lower in the left triangular part of the inferior frontal gyrus, right DLPFC, right SMA, bilateral PCC and the orbital part of the middle frontal gyrus, and higher in the left middle temporal gyrus and left caudate nucleus. These regions mainly belong to the default mode network (DMN), executive control network and primary sensory motor network. The results of the correlation analysis indicated a positive association between the changes in IIEF5 score and changes in the fALFF value in the right PCC after EA treatment.

Conclusion

In conclusion, our study highlights that PED patients have abnormal patterns of activity in the right PCC, right DLPFC, and right SMA mainly involved in the DMN, executive central network, and sensory motor network which could lead to a higher levels of sexual inhibition. EA might regulate the process of sexual inhibition to improve erection function in PED patients probably by modulating spontaneous brain activity in the DMN, executive central network, and sensory motor network.
A comparative analysis of sleep spindle characteristics of sleep-disordered patients and normal subjects
Authors: Chen Chao , Wang Kun , Belkacem Abdelkader Nasreddine , Lu Lin , Yi Weibo , Liang Jun , Huang Zhaoyang , Ming Dong
Published: March 2023

Spindles differ in density, amplitude, and frequency, and these variations reflect different physiological processes. Sleep disorders are characterized by difficulty in falling asleep and maintaining sleep. In this study, we proposed a new spindle wave detection algorithm, which was more effective compared with traditional detection algorithms such as wavelet algorithm. Besides, we recorded EEG data from 20 subjects with sleep disorders and 10 normal subjects, and then we compared the spindle characteristics of sleep-disordered subjects and normal subjects (those without any sleep disorder) to assess the spindle activity during human sleep. Specifically, we scored 30 subjects on the Pittsburgh Sleep Quality Index and then analyzed the association between their sleep quality scores and spindle characteristics, reflecting the effect of sleep disorders on spindle characteristics. We found a significant correlation between the sleep quality score and spindle density (p = 1.84 × 10−8, p-value <0.05 was considered statistically significant.). We, therefore, concluded that the higher the spindle density, the better the sleep quality. The correlation analysis between the sleep quality score and mean frequency of spindles yielded a p-value of 0.667, suggesting that the spindle frequency and sleep quality score were not significantly correlated. The p-value between the sleep quality score and spindle amplitude was 1.33 × 10−4, indicating that the mean amplitude of the spindle decreases as the score increases, and the mean spindle amplitude is generally slightly higher in the normal population than in the sleep-disordered population. The normal and sleep-disordered groups did not show obvious differences in the number of spindles between symmetric channels C3/C4 and F3/F4. The difference in the density and amplitude of the spindles proposed in this paper can be a reference characteristic for the diagnosis of sleep disorders and provide valuable objective evidence for clinical diagnosis. In summary, our proposed detection method can effectively improve the accuracy of sleep spindle wave detection with stable performance. Meanwhile, our study shows that the spindle density, frequency and amplitude are different between the sleep-disordered and normal populations.
Intermittent theta burst stimulation vs. high-frequency repetitive transcranial magnetic stimulation for post-stroke cognitive impairment: Protocol of a pilot randomized controlled double-blind trial
Authors: Han Menglin , He Jinyan , Chen Na , Gao Yulan , Wang Zhiqiang , Wang Kangling
Published: March 2023

Introduction

Intermittent theta burst stimulation (iTBS), a novel mode of transcranial magnetic stimulation (TMS), has curative effects on patients with post-stroke cognitive impairment (PSCI). However, whether iTBS will be more applicable in clinical use than conventional high-frequency repetitive transcranial magnetic stimulation (rTMS) is unknown. Our study aims to compare the difference in effect between iTBS and rTMS in treating PSCI based on a randomized controlled trial, as well as to determine its safety and tolerability, and to further explore the underlying neural mechanism.

Methods

The study protocol is designed as a single-center, double-blind, randomized controlled trial. Forty patients with PSCI will be randomly assigned to two different TMS groups, one with iTBS and the other with 5 Hz rTMS. Neuropsychological evaluation, activities of daily living, and resting electroencephalography will be conducted before treatment, immediately post-treatment, and 1 month after iTBS/rTMS stimulation. The primary outcome is the change in the Montreal Cognitive Assessment Beijing Version (MoCA-BJ) score from baseline to the end of the intervention (D11). The secondary outcomes comprise changes in resting electroencephalogram (EEG) indexes from baseline to the end of the intervention (D11) as well as the Auditory Verbal Learning Test, the symbol digit modality test, the Digital Span Test findings, and the MoCA-BJ scores from baseline to endpoint (W6).

Discussion

In this study, the effects of iTBS and rTMS will be evaluated using cognitive function scales in patients with PSCI as well as data from resting EEG, which allows for an in-depth exploration of underlying neural oscillations. In the future, these results may contribute to the application of iTBS for cognitive rehabilitation of patients with PSCI.
Whole-transcriptome sequencing identifies key differentially expressed circRNAs/lncRNAs/miRNAs/mRNAs and linked ceRNA networks in adult degenerative scoliosis
Authors: Shi Xin , Li Panpan , Wu Xiang , Shu Jun
Published: March 2023

Background

Adult degenerative scoliosis (ADS) is forecast to be a prevalent disabling condition in an aging society. Universally, its pathogenesis is perceived as intervertebral disc degeneration (IDD), however, a thought-provoking issue is why precisely a subset of patients with disc degeneration develop ADS. Exploring the diversities between common IDD and ADS would contribute to unraveling the etiological mechanisms of ADS. Therefore, we aimed to integrate the circRNA, lncRNA, miRNA, and mRNA expression profiles from normal adults (Normal), patients with lumbar disc herniation (LDH), and ADS by whole transcriptome sequencing, which identifies critical functional ncRNA and ceRNA networks and crosstalk between the various transcripts.

Methods

The fresh whole blood samples (n = 3/group) were collected from ADS patients, LDH patients, and healthy volunteers (Normal group), which were examined for mRNA, miRNA, lncRNA, and circRNA expression and screened for differentially expressed (DE) ncRNAs. Then, Gene Ontology (GO) and KEGG analyses were performed for gene annotation and enrichment pathways on the DE RNAs, which were constructed as a lncRNA-miRNA-mRNA network. Eventually, DE RNAs were validated by qRT-PCR targeting disc nucleus pulposus (NP) tissue in ADS and LDH group (n = 10/group).

Results

Compared to the LDH group, we identified 3322 DE mRNAs, 221 DE lncRNAs, 20 DE miRNAs, and 15 DE circRNAs in the ADS. In contrast to Normal, 21 miRNAs and 19 circRNAs were differentially expressed in the ADS. The expression of multiple differentially expressed ncRNAs was confirmed by qRT-PCR analysis to be consistent with the sequencing results. In addition, GO, and KEGG analysis demonstrated that most DE mRNAs and ncRNAs target genes are involved in various biological processes, including Endocytosis, Apoptosis, Rap1 signaling pathway, Notch signaling pathway, and others. The constructed lncRNA-miRNA-mRNA co-expression network was primarily related to angiogenesis and regulation.

Conclusion

By focusing on comparing asymmetric and symmetric disc degeneration, whole-transcriptome sequencing and bioinformatics analysis systematically screened for key ncRNAs in the development of ADS, which provided an abundance of valuable candidates for the elucidation of regulatory mechanisms. The DE ncRNAs and the lncRNA-miRNA-mRNA network are intrinsically involved in the regulation of mediator and angiogenesis, which may contribute to the insight into the pathogenesis of ADS.
Primary squamous cell carcinoma of renal parenchyma: A case report and literature review
Authors: Liang Ke , Yuan Yichu , Lv Bodong , Ke Zunjin
Published: March 2023

Rationale

Primary renal parenchymal squamous cell carcinoma (SCC) is an extremely rare tumor that is difficult to diagnose by hematology and imaging studies and is often diagnosed later than other primary renal cancers.

Diagnosis

A 52-year-old male patient was found to have cysts in both kidneys for 1 week. No urgency and frequency of urination, no dysuria, no gross hematuria, and no significant changes in recent body weight were reported.

Interventions

The upper pole of the right kidney is a cystic and solid mass (8.3 cm * 8.2 cm * 8.1 cm), the cystic part has long T1 and long T2 signals, the solid part has mixed signals, and some parts have limited diffusion. There were nodular long T1 and short T2 calcification signals. An enhanced scan of the solid part showed uneven enhancement and continuous enhancement of the mass capsule. Cystic renal cancer was considered because of the multiple cysts in both kidneys. Surgical treatment was performed. Postoperative pathology revealed well-differentiated squamous cell carcinoma of the right kidney with cystic degeneration, 8.5 cm * 6 cm in size, infiltrating the renal parenchyma, and the cutting edge was negative. The pathological stage was pT2bN0M0.

Outcome

At the follow-up 5 months after the operation, no metastasis was found.

Conclusion

Renal SCC is rare and easily misdiagnosed and missed. Pathological diagnosis is still the gold standard for its diagnosis. However, with active surgical treatment, the short-term prognosis of the patient is good.
Mu opioid receptor mRNA overexpression predicts poor prognosis among 18 common solid cancers: A pan-cancer analysis
Authors: Sun Wei , Zhuang Shaohui , Cheng Minghua , Qiu Zeting
Published: March 2023

Background

Opioids are widely used for patients with solid tumors during surgery and for cancer pain relief. We conducted a pan-cancer genomic analysis to investigate the prognostic features of Mu opioid receptor (MOR) mRNA expression across 18 primary solid cancers.

Methods

All the data of cancer with MOR mRNA were retrieved from cBioPortal for Cancer Genomics. Logistic regression was used to determine the associations between MOR mRNA expression and clinicopathological features. Log-rank test and Cox regression was used for survival analysis. Subgroup analysis and propensity score matching were also carried out.

Results

7,274 patients, including 1,112 patients with positive MOR mRNA expression, were included for data analyses. Positive MOR mRNA expression was associated with more advanced stage of T (adjusted Odds ratio [OR], 1.176; 95% confidence interval [CI], 1.022-1.354; P=0.024), M (adjusted OR, 1.548; 95% CI, 1.095-2.189; P=0.013) except N (adjusted OR, 1.145; 95% CI, 0.975-1.346; P=0.101), and worse prognosis for overall survival (Hazard ratio [HR] 1.347, 95% CI 1.200-1.512, P<0.001), progression-free survival (HR 1.359, 95% CI 1.220-1.513, P<0.001), disease-free survival (HR 1.269, 95% CI 1.016-1.585, P<0.001) and disease-specific survival (HR 1.474, 95% CI 1.284-1.693, P<0.001). Patients with positive MOR mRNA expression tended to be classified as tumor microenvironment immune types II, representing low PD-L1 and low CD8A expression.

Conclusion

MOR mRNA overexpression is associated with poor prognosis and poor response to PD-L1 therapy.
Rotating detonation combustors for propulsion: Some fundamental, numerical and experimental aspects
Authors: Le Naour Bruno , Davidenko Dmitry , Gaillard Thomas , Vidal Pierre
Published: March 2023

Propulsion systems based on the constant-pressure combustion process have reached maturity in terms of performance, which is close to its theoretical limit. Technological breakthroughs are needed to develop more efficient transportation systems that meet today’s demands for reduced environmental impact and increased performance. The Rotating Detonation Engine (RDE), a specific implementation of the detonation process, appears today as a promising candidate due to its high thermal efficiency, wide operating Mach range, short combustion time and, thus, high compactness. Following the first proofs of concept presented in the 1960s, the last decade has seen a significant increase in laboratory demonstrators with different fuels, injection techniques, operating conditions, dimensions and geometric configurations. Recently, two flight tests of rocket-type RDEs have been reported in Japan and Poland, supervized by Professors Kasahara (Nagoya University) and Wolanski (Warsaw University), respectively. Engineering approaches are now required to design industrial systems whose missions impose efficiency and reliability constraints. The latter may render ineffective the simplified solutions and configurations developed under laboratory conditions. This requires understanding the fundamentals of detonation dynamics relevant to the RDE and the interrelated optimizations of the device components. This article summarizes some of the authors’ experimental and numerical work on fundamental and applied issues now considered to affect, individually or in combination, the efficiency and reliability of the RDE. These are the structure of the detonation reaction zone, the detonation dynamics for rotating regimes, the injection configurations, the chamber geometry, and the integration constraints.
Management of dysmenorrhea through yoga: A narrative review
Authors: Kanchibhotla Divya , Subramanian Saumya , Singh Deeksha
Published: March 2023

Menstrual pain also known as dysmenorrhea is one of the most common and underrated gynecological disorders affecting menstruating women. Although the symptoms and impact might vary greatly, it is defined by cramps in the lower abdomen and pain that radiates to lower back and thighs. In some cases it is also accompanied by nausea, loose stool, dizziness etc. A primary narrative review was conducted on the impact of yoga on dysmenorrhea experienced by women. The English-language literature published until 2022 was searched across databases such as PubMed, Google Scholar and Scopus. “Yoga” and “menstrual pain” OR “dysmenorrhea” were used as keywords for the search across several databases. A total of 816 title searches were obtained across all the database searches. This review article included 10 studies based on the selection criteria. The studies examined the impact of a varied set of 39 asanas, 5 pranayamas and Yoga nidra on dysmenorrhea. The studies demonstrated a significant relief in dysmenorrhea among those who practiced Yoga (asanas/pranayama/yoga nidra) with improved pain tolerance and reduced stress levels. Regulating the stress pathways through yoga was found to be a key in regulating hormonal balance and reduction in dysmenorrhea.
COVID-19 pandemic’s disproportionate impact on childhood bereavement for youth of color: Reflections and recommendations
Authors: Burns Michaeleen , Landry Laura , Mills David , Carlson Nichole , Blueford Jillian M. , Talmi Ayelet
Published: March 2023

The COVID-19 pandemic devastated public welfare worldwide, bringing excess deaths connected to causes such as homicide, substance abuse, and heart disease. In the U.S., these mortality increases disproportionally impacted communities of color and contributed to a rise in bereavement among adults and children. The death of an important person is one of the most frequently reported disruptive childhood experiences. According to 2023 Childhood Bereavement Estimation Model (CBEM) results, one in 14 U.S. children will experience the death of a parent by age 18. The current study analyzes the impact of the pandemic on childhood bereavement due to parent death by comparing CBEM results for 2021 and 2020 to the average of annual results for 2016 through 2019 for combined U.S. Census race and Hispanic origin categories. Analyses demonstrate that more than 700,000 U.S. children were newly bereaved due to a parent's death in 2020 and 2021. 2020 increases were observed for each race and Hispanic origin population, ranging from 14.9% to 72.4% compared to the 2016–2019 annual average. Hispanic Asian Pacific Islander and Hispanic Black youth experienced the largest percentage increases, while non-Hispanic white youth experienced the smallest. The results contribute to the growing evidence documenting longstanding and enduring disparities in critical U.S. health outcomes based on race and Hispanic origin. Recommendations for the scale and focus of efforts to understand and address bereavement in a way that accommodates the rising need for support in diverse populations to help all bereaved children find hope and healing are offered.
Bartter syndrome type III with glomerular dysplasia and chronic kidney disease: A case report
Authors: Liu Jingqi , Zhang Yudi , Wu Xiaochuan , Li Yongzhen
Published: March 2023

Background

Bartter syndrome (BS) type III is a rare autosomal recessive genetic disease. Its clinical features are polyuria, hypokalemia, hypochloremia, metabolic alkalosis, and hyperreninaemia. A few BS type III can be complicated with chronic kidney disease.

Case presentation

We report a 14-year-old boy with Bartter syndrome caused by a c.1792C > T (p.Q598*) mutation in the CLCNKB gene. He was a no deafness and full-term baby, and he had renal dysplasia and chronic kidney disease (CKD). In addition, we summarize all cases of BS type III complicated with CKD.

Conclusions

We report a case of Bartter syndrome complicated by chronic kidney disease caused by a new mutation of CLCNKB. As we all know, BS type IV is usually combined with chronic kidney disease, and BS type III can also integrate with CKD. We don't find BS type III with glomerular dysplasia in the literature. So renal damage in BS type III is not only FSGS; clinicians must also be aware of glomerular dysplasia.
Current, emerging, and potential therapies for non-alcoholic steatohepatitis
Authors: Yang Zhen , Wang Lin
Published: March 2023

Non-alcoholic fatty liver disease (NAFLD) has been identified as the most common chronic liver disease worldwide, with a growing incidence. NAFLD is considered the hepatic manifestation of a metabolic syndrome that emerges from multiple factors (e.g., oxidative stress, metabolic disorders, endoplasmic reticulum stress, cell death, and inflammation). Non-alcoholic steatohepatitis (NASH), an advanced form of NAFLD, has been reported to be a leading cause of cirrhosis and hepatic carcinoma, and it is progressing rapidly. Since there is no approved pharmacotherapy for NASH, a considerable number of therapeutic targets have emerged with the deepening of the research on NASH pathogenesis. In this study, the therapeutic potential and properties of regulating metabolism, the gut microbiome, antioxidant, microRNA, inhibiting apoptosis, targeting ferroptosis, and stem cell-based therapy in NASH are reviewed and evaluated. Since the single-drug treatment of NASH is affected by individual heterogeneous responses and side effects, it is imperative to precisely carry out targeted therapy with low toxicity. Lastly, targeted therapeutic agent delivery based on exosomes is proposed in this study, such that drugs with different mechanisms can be incorporated to generate high-efficiency and low-toxicity individualized medicine.
Study and application of temporary plugging agent for temporary plugging acid fracturing in ultra-deep wells of Penglai gas field
Authors: Wang Yang , Fan Yu , Chen Weihua
Published: March 2023

The burial depth of Penglai gas field reservoir in the Sichuan Basin of China exceeds 7,000 m, and the reservoir temperature is 160°C. Penglai gas field belongs to the fractured reservoir, and there are many natural fractures distributed in the reservoir. Natural fractures are not only the storage place of natural gas, but also the transportation channel of natural gas. Gas wells mainly increase natural gas production through acid fracturing. In the early stage, Penglai Gas Field mainly used gelled acid fracturing technology to create an artificial fracture in the reservoir by injecting a large amount of acid fluid. However, the stimulation range of gelled acid fracturing is very small. After acid fracturing, gas wells can only produce 120,000 cubic meters of natural gas per day. To obtain higher natural gas production, this paper proposes a multi-stage temporary acid fracturing technology that can greatly improve the effect of acid fracturing. The temporary plugging agent for acid fracturing has been developed to meet the high-temperature requirements of the Penglai gas field. Based on the simulation of fracture propagation, the feasibility of acid fracturing expansion of natural fractures in the Penglai Gas Field is clarified. Fiber and granular temporary plugging agents that meet the plugging strength greater than 20 MPa are selected by using the dynamic temporary plugging instrument. MultiFracS software is used to optimize the multi-stage temporary acid fracturing process parameters. The research results in this paper have been applied to wells PS101, PS102, and PS103. After fracturing, compared with the gelling acid fracturing, the natural gas production has increased by more than three times, and the multi-stage temporary plugging acid fracturing has achieved a very good stimulation effect.