bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding behaviour change to promote regular dental attendance
Authors: Holloway Jessica
Published: November 2021

Regular dental attendance is a key oral health behaviour. Behaviour change interventions are increasingly being used to promote positive oral health behaviours. A systematic approach to understanding behaviour has led to the development of frameworks which aim to guide the process of designing behaviour change interventions. One such framework is the Behaviour Change Wheel (BCW). This article aims to explore and identify barriers to regular dental attendance which may be targeted using behaviour change interventions based on the Capability, Opportunity and Motivation Behaviour model (COM-B) and the BCW, and suggests potential behaviour change techniques which could be utilised into a behaviour change intervention with the aim to promote regular dental attendance.
COVID-19 Pandemic: The Urgent Dental Hub experience from a primary care perspective
Authors: Khwaja Zohaib , Ali Awais , Rai Manraj
Published: November 2021

In response to the nationwide lockdown on 23 March 2020 in the UK, urgent dental hubs (UDHs) were established in the community to provide emergency dental care.

Consecutive referrals to a primary care UDH were prospectively analysed over a one-month period, from 18 May 2020 to 18 June 2020.

Of 400 referrals received, the most common were in relation to pain (87%). In 63% neither a radiograph nor photograph was provided with the referral. Seventy percent of patients were telephone triaged within 24 hours of receipt of referral. Fifty-three percent of referrals were accepted for face-to-face treatment, of which 69% were treated by extraction. Of rejected referrals (n=179; 45%), 79% were due to symptoms having settled or being manageable by the time of triage. A small number of referrals were redirected for specialist care. Referrals that were accepted were more likely to have been prescribed antibiotics and less likely to have been referred by the general dental practitioner (GDP) they regularly saw (p <0.01).

Patients that were older and those that identified themselves as not having a regular GDP were less likely to have been referred to an UDH. The quality of referrals was poor and there may be a role for virtual consultations moving forwards. We found pre-referral antimicrobial prescriptions were high and a confused public health message may have been sent.
The Implementation of Mindfulness-Based Programs in the Swedish Healthcare System – A Survey Study of Service Providers
Authors: Niemi Maria , Crane Rebecca , Sinselmeijer Jermo , Andermo Susanne
Published: November 2021

Background

The burden of depression and anxiety is on the rise globally. Mindfulness-Based Programs (MBPs) are a particular group of psychosocial programs targeting depression and anxiety. There is growing research and practice interest in MBPs internationally, and they are becoming more commonly implemented in a number of countries’ healthcare services.

Objective

To systematically map the existing provision of MBPs in the Swedish healthcare sector, in order to understand facilitators and barriers to uptake, and so inform future implementation efforts.

Methods

We assessed the experiences of MBP implementation among relevant stakeholders in Swedish healthcare settings through an online survey. The survey was designed to gather data on (1) the evidence-base of practice being implemented; (2) the context in which implementation was taking place and (3) the process of facilitation. Respondents were identified through snowball sampling of key stakeholders.

Results

In total, 129 individuals from 20 of the 21 healthcare regions in Sweden responded to the survey. Our findings showed that there is variation in the types of MBP models being implemented, and that the delivery structure of evidence-based programs were often being modified for implementation. We found some divergence from international guidance on good practice standards for the training of MBP teachers within Swedish implementation processes. The main service context for implementation is primary care; the most important facilitating factors for successful MBP implementation were the presence of a championing individual and support from leadership. The most influential hindering factors for implementation were lack of time, and lack of funding.

Conclusion

To support integrity and fidelity of MBP implementation in Sweden, a strategic plan and good practice guidelines seem necessary. Also, an evidence-based stepped care model for implementation may work to ensure intervention fidelity in cases where time and funding constraints permit.
Sex-Based Differences in the Drop Vertical Jump as Revealed by Video Motion Capture Analysis Using Artificial Intelligence
Authors: Kawaguchi Kohei , Taketomi Shuji , Mizutani Yuri , Uchiyama Emiko , Ikegami Yosuke , Tanaka Sakae , Haga Nobuhiko , Nakamura Yoshihiko
Published: November 2021

Background:

Sex-based biomechanical differences during a drop vertical jump (DVJ) may explain the increased risk of anterior cruciate ligament injury in females. Video motion capture using artificial intelligence (VMocap) is a new method for accurate motion analysis.

Purpose:

To use VMocap to identify sex-based differences in biomechanics during a DVJ in Asian athletes.

Study Design:

Controlled laboratory study.

Methods:

A total of 63 female and 61 male Asian soccer players volunteered for this study in 2018. Participants performed a bilateral DVJ using VMocap, and the knee valgus angle (KVA), knee flexion angle (KFA), hip flexion angle (HFA), and lower leg anterior inclination angle (LAIA) were calculated from the motion capture data. These joint angles and inclination angles were evaluated at the time of highest point of the first jump (H1), initial contact (IC), maximum knee flexion (MKF), toe-off (TO), and highest point of the second jump (H2). The unpaired t test was used to compare sex-based differences.

Results:

At H1, the KVA in females showed more valgus (−2.9° vs −5.4°) and the LAIA in females was greater (29.1° vs 25.7°) versus males ( P < .01 for both). At IC, the KVA in females showed more valgus (−1.3° vs −3.0°) and females had a greater KFA (20.8° vs 14.3°) and LAIA (5.1° vs 0.0°) compared with males ( P < .01 for all). At MKF, female KVA showed more valgus (6.2° vs −9.5°), and females had greater LAIA (36.6° vs 34.6°), smaller KFA (77.5° vs 87.5°), and smaller HFA (55.8° vs 82.0°) compared with males ( P < .01 for all). At TO, female KVA showed more valgus (−0.7° vs −3.1°) and female KFA, HFA, and LAIA were greater (31.7° vs 19.2°; 19.9° vs 16.4°; and 18.2° vs 11.5°, respectively) than males ( P < .01 for all). At H2, females had a greater KFA (18.6° vs 14.6°) and LAIA (13.3° vs 9.9°) than males ( P < .04 for both).

Conclusion:

Asian female soccer players showed increased KVA and LAIA, decreased KFA and HFA at MKF, and increased KFA at IC and TO compared with their male counterparts in this analysis of the DVJ.

Clinical Relevance:

Elucidation of kinematic differences between the sexes can aid in predicting injuries.
Outcomes After Arthroscopic Rotator Cuff Repair Using Margin Convergence Versus Superior Capsular Reconstruction: Should Candidates for Margin Convergence Be Treated With Superior Capsular Reconstruction?
Authors: Ciccotti Michael , Horan Marilee P. , Nolte Philip-C. , Elrick Bryant P. , Millett Peter J.
Published: November 2021

Background:

Both margin convergence rotator cuff repair (MC-RCR) and superior capsular reconstruction (SCR) result in improved clinical outcomes in the treatment of massive rotator cuff tears (RCTs). The question remains whether it is better to perform MC-RCR using native, albeit occasionally deficient, tissues or to perform primary SCR.

Purpose/Hypothesis:

To compare the clinical results of MC-RCR versus SCR for the treatment of massive RCTs. It was hypothesized that SCR would yield better outcomes.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

Included were patients who underwent arthroscopic MC-RCR or SCR for massive RCTs performed by a single surgeon between 2014 and 2019. MC-RCR was performed if it was technically possible to close the defect; otherwise, SCR was performed. Outcomes were assessed at 6 months and then annually using American Shoulder and Elbow Surgeons; Single Assessment Numerical Evaluation; shortened version of Disabilities of the Arm, Shoulder and Hand; 12-Item Short Form Health Survey Physical Component Summary; and patient satisfaction scores. The minimal clinically important difference (MCID), substantial clinical benefit (SCB), and Patient Acceptable Symptom State (PASS) were compared between groups. Revision surgeries and complications were reported.

Results:

Included were 46 patients in the MC-RCR group (mean age, 59 ± 8 years) and 46 patients in the SCR group (mean age, 57 ± 7 years); 29 patients in each group were available at 2-year follow-up. Preoperative outcome scores were not significantly different between groups. Within groups, all outcome scores improved from pre- to postoperatively ( P < .05), with no significant differences in postoperative scores or patient satisfaction between groups. No significant between-group differences were noted in the percentage of patients who reached the MCID, SCB, and PASS (MCID, 92.3% vs 84.6%; SCB, 80.8% vs 80.8%; and PASS, 66.7% vs 66.7%). SCR had a significantly lower survivorship rate compared with MC-RCR (84.7% vs 100%) ( P = .026).

Conclusion:

Both MC-RCR and SCR provided similar improvement in outcomes; however, SCR resulted in a significantly lower survivorship rate at 2 years postoperatively. If an RCT is technically repairable, we recommend that it be repaired primarily, even if MC techniques are needed to close the defect. SCR remains a good option for massive RCTs that are not technically repairable.
Product Placement Bibliometric Study: Generic Journals Versus Specific-Communication Journals
Authors: Vila-López Natalia , Kuster-Boluda Ines
Published: November 2021

Media fragmentation represents new challenges for product placement strategies to become an increasingly effective way to reach consumers and non-users. In this frame, this paper has been developed with three main objectives: (i) to carry on a performance analysis to measure the visibility/impact of the scientific product in product placement (most cited authors, journals, and themes), (ii) to visually present the scientific structure by topics of research in product placement as well as its evolution to identify future research lines, and (iii) to compare both objectives in generic journals and specific communication ones. To this end, the resources in the Web of Science Citation Index were used. Scimat software was applied on a sample of 694 indexed papers from 1992 to 2021 containing “product placement” with 8,521 global citations (176 of the papers were indexed in communication journals with 3,190 citations). Our results show that MEMORY is a key motor theme—the future of research tends to new themes in the communication field (i.e., ATTITUDES/BEHAVIORS or VIRTUAL). Three industries have been key: alcohol, tobacco, and food. This research adds value to previous analysis as long as we have included: (i) a multidisciplinary approach; (ii) an unfolded analysis focusing strictly on communication journals; and (iii) a longitudinal analysis to compare different periods showing dynamic scientific maps.
Race, Criminal Records, and Discrimination Against Job Seekers: Examining Attitudinal Mechanisms
Authors: Denver Megan , Pickett Justin T.
Published: November 2021

Regardless of why it happens, racial discrimination is damaging and unacceptable. Efforts to reduce discrimination, however, are most successful when we understand the mechanisms that give rise to it. Building on the observation that employers are members of the public, we examine two attitudinal mechanisms that may foster discriminatory employment practices in the context of criminal background checks: stereotypes and threat-based animus. First, we estimate public perceptions of arrest prevalence using two nationwide surveys. Next, we experimentally test the effects of two racially threatening primes—Census projections about a coming majority-minority America, and information about the prison population’s racial composition—on attitudes toward hiring job applicants with criminal records. Consistent with statistical discrimination theory, respondents identify black males as having the highest arrest prevalence. Respondents are less accurate, however, when it comes to gender differences: they underestimate arrest prevalence for black, Hispanic, and white males, and tend to overestimate it for females. On the other hand, our experiments provide little evidence of an effect of threat-based animus: racially threatening primes that are influential in other contexts do not significantly impact attitudes about hiring applicants with criminal records.
Out of the Class and Into the Shadows: Immigration Enforcement and Education Among U.S.-Citizen and Foreign-Born Hispanics
Authors: Bucheli Jose R. , Rubalcaba Joaquín Alfredo-Angel , Vargas Edward D.
Published: November 2021

With the recent escalation in interior immigration enforcement across the United States, immigrant and U.S.-born children are increasingly exposed to coercive measures that have been shown to disrupt their development. This study examines the relationship between immigration-related arrests and the educational outcomes of Hispanics—a group that is overwhelmingly targeted by immigration authorities. Using data on the number of U.S. Immigration and Customs Enforcement arrests at the Metropolitan Statistical Area level, we estimate the impact of immigration enforcement on Hispanics’ school enrollment. We find that increases in the arrest rate are accompanied by substantial declines in enrollment among Hispanic youth, including U.S.-born, foreign-born, and individuals in mixed-status families. Additionally, we do not find evidence of this relationship among other racial/ethnic groups, suggesting that the impact is concentrated among Hispanic individuals. Our results advance our understanding of the unintended consequences of immigration enforcement on educational outcomes and show that ethnicity is a crucial factor in this process.
Trends in U.S. Residential Racial Segregation, 1990 to 2020
Authors: Elbers Benjamin
Published: November 2021

This data visualization presents changes in U.S. residential racial segregation from 1990 to 2020, including the recently released 2020 census data. Using Theil’s information index H, the visualization shows both the multigroup index of segregation that involves all racial groups and also all possible pairwise indices of the four major racial groups: whites, Blacks, Hispanics, and Asians. Although multigroup segregation declined by about 12 percent to 16 percent in every decade, the results for some racial groups are more mixed. The segregation of Blacks from all other groups declined over the entire period, but the segregation of Hispanics and Asians from the white population increased, as did the segregation between Hispanics and Asians. For the most recent period, 2010 to 2020, all pairwise segregation indices declined by between 7 percent and 14 percent, except Asian-white segregation, which increased by about 3 percent. Despite these declines, Blacks in particular remain highly segregated from whites and Asians in many U.S. metropolitan areas.
Coming Out (or not) on College Applications: Institutional and Interpersonal Dimensions of Disclosing LGBQ+ Identities
Authors: Van Dyke Nella , Hagaman Natasha , Beattie Irenee R. , Alnagar Hala
Published: November 2021

Many college campuses recently began asking undergraduate applicants about their sexual identities on their college applications. How do applicants who are lesbian, gay, bisexual, and otherwise on the queer spectrum (LGBQ+) experience this question, and what factors influence how they respond? The authors use focus group and interview data with 60 LGBQ+ undergraduate students attending two college campuses to explore these questions. Although many students were comfortable with coming out, they described evaluating the potential risks and benefits of disclosure, and some lacked trust in the institution and were concerned about loss of information control. Unlike when coming out in other institutions, many expressed serious concerns about how the information might get back to their parents. Such spillover effects from institutional disclosure have not been previously identified in the literature. These concerns were heightened for students of color. The present results extend theoretical understandings of coming out and offer valuable insights to college administrators.
Teaching Psychopharmacology in the Medically Ill: A Problem-Based Learning Card Game for Consultation-Liaison Psychiatry Didactics
Authors: Fipps David C. , Rainey Elisabet
Published: November 2021

INTRODUCTION

Our educational report discusses a unique card game that provides an engaging, and competitive strategy for teaching psychopharmacology in the medically ill. It engages the players and helps them learn how to justify medical decisions in the context of complicated medical comorbidity.

METHODS

We describe a problem-based learning approach where learners are presented with randomized diagnoses and complications and are then forced to make prescription decisions for treatment from a limited and randomized supply of medication cards. Each round is facilitated by the teacher, who engages the teams and/or individual players in discussions regarding the rationale and justification of the medication decisions. These treatment plans are scored according to the appropriateness of the medication choice and the process is repeated.

DISCUSSION

The game is flexible regarding players’ levels of education and has been played by medical students, psychiatry residents, and fellows throughout their years of training. Overall feedback has been positive from facilitators and trainees and the game has been a valuable source of engaging learners in the process of making complex medication decisions in the medically ill.
Vitreoretinal Lymphoma as the Initial Presentation of a Breast Diffuse Large B-cell Lymphoma
Authors: Martin Cole A. , Admirand Joan H. , Ghafoori Shelley Day
Published: November 2021

Purpose:

We report a case of vitreoretinal lymphoma as the initial presentation of diffuse large B-cell lymphoma of the breast.

Methods:

A case report is presented.

Results:

A 72-year-old woman was referred for evaluation of vitritis in both eyes. Diagnostic vitrectomy and pathology workup demonstrated atypical lymphoid cells consistent with the presence of diffuse large B-cell lymphoma. Brain magnetic resonance imaging and lumbar puncture findings were negative for central nervous system lymphoma. Positron emission tomography/computed tomography imaging showed a mass in the left breast, and breast biopsy confirmed aggressive B-cell lymphoma.

Conclusions:

When vitreoretinal lymphoma is diagnosed, it is important to evaluate for other forms of systemic lymphoma in addition to central nervous system lymphoma.
“Messy Inclusion”: A Call for Dignity of Risk in Inclusive Postsecondary Education
Authors: Bumble Jennifer L. , Worth Christopher R. J. , Athamanah Lindsay S. , Rooney-Kron Magen , Regester April , Lidgus Jonathan
Published: November 2021

As inclusive postsecondary education (IPSE) programs emerge and expand across the United States, many young people with intellectual and developmental disabilities (IDD) have access to higher education for the first time. To support growth and identity development, students with IDD should have access to all aspects of college life (e.g., activities, relationships, opportunities). Furthermore, students with IDD should have the freedom to take emotional, physical, and intellectual risks relative to those of their peers without disabilities. This leading edge article is written by practitioners and researchers with and without disabilities associated with the University of Missouri St. Louis IPSE program, Succeed. To center the voices of persons with disabilities, any direct quotations are in italics. In this article, we advocate for increased dignity of risk in IPSE, present a model to assess and make programmatic decisions regarding dignity of risk in IPSE, and highlight five philosophical values that guide our work toward “messy inclusion.”
lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway
Authors: Yu Xiao , Rong Peng-Ze , Song Meng-Sheng , Shi Ze-Wen , Feng Gong , Chen Xian-Jun , Shi Lin , Wang Cheng-Hao , Pang Qing-Jiang
Published: November 2021

Background

We aimed to investigate the functions and underlying mechanism of lncRNA SNHG1 in bone differentiation and angiogenesis in the development of osteoporosis.

Methods

The differential gene or proteins expressions were measured by qPCR or western blot assays, respectively. The targeted relationships among molecular were confirmed through luciferase reporter, RIP and ChIP assays, respectively. Alkaline phosphatase (ALP), alizarin red S (ARS) and TRAP staining were performed to measure the osteoblast/osteoclast differentiation of BMSCs. The viability, migration and angiogenesis in BM-EPCs were validated by CCK-8, clone formation, transwell and tube formation assays, respectively. Western blot and immunofluorescence detected the cytosolic/nuclear localization of β-catenin. Ovariectomized (OVX) mice were established to confirm the findings in vitro.

Results

SNHG1 was enhanced and miR-181c-5p was decreased in serum and femoral tissue from OVX mice. SNHG1 directly inhibited miR-181c-5p to activate Wnt3a/β-catenin signaling by upregulating SFRP1. In addition, knockdown of SNHG1 promoted the osteogenic differentiation of BMSCs by increasing miR-181c-5p. In contrast, SNHG1 overexpression advanced the osteoclast differentiation of BMSCs and inhibited the angiogenesis of BM-EPCs, whereas these effects were all reversed by miR-181c-5p overexpression. In vivo experiments indicated that SNHG1 silencing alleviated osteoporosis through stimulating osteoblastogenesis and inhibiting osteoclastogenesis by modulating miR-181c-5p. Importantly, SNHG1 could be induced by SP1 in BMSCs.

Conclusions

Collectively, SP1-induced SNHG1 modulated SFRP1/Wnt/β-catenin signaling pathway via sponging miR-181c-5p, thereby inhibiting osteoblast differentiation and angiogenesis while promoting osteoclast formation. Further, SNHG1 silence might provide a potential treatment for osteoporosis.

Graphic abstract
Weighted gene co-expression network-based approach to identify key genes associated with anthracycline-induced cardiotoxicity and construction of miRNA-transcription factor-gene regulatory network
Authors: Wan Guoxing , Chen Peinan , Sun Xue , Cai Xiaojun , Yu Xiongjie , Wang Xianhe , Cao Fengjun
Published: November 2021

Background

Cardiotoxicity is a common complication following anthracycline chemotherapy and represents one of the serious adverse reactions affecting life, which severely limits the effective use of anthracyclines in cancer therapy. Although some genes have been investigated by individual studies, the comprehensive analysis of key genes and molecular regulatory network in anthracyclines-induced cardiotoxicity (AIC) is lacking but urgently needed.

Methods

The present study integrating several transcription profiling datasets aimed to identify key genes associated with AIC by weighted correlation network analysis (WGCNA) and differentially expressed analysis (DEA) and also constructed miRNA-transcription factor-gene regulatory network. A total of three transcription profiling datasets involving 47 samples comprising 41 rat heart tissues and 6 human induced pluripotent stem cell-derived cardiomyocytes (hiPSCMs) samples were enrolled.

Results

The WGCNA and DEA with E-MTAB-1168 identified 14 common genes affected by doxorubicin administrated by 4 weeks or 6 weeks. Functional and signal enrichment analyses revealed that these genes were mainly enriched in the regulation of heart contraction, muscle contraction, heart process, and oxytocin signaling pathway. Ten (Ryr2, Casq1, Fcgr2b, Postn, Tceal5, Ccn2, Tnfrsf12a, Mybpc2, Ankrd23, Scn3b) of the 14 genes were verified by another gene expression profile GSE154603. Importantly, three key genes (Ryr2, Tnfrsf12a, Scn3b) were further validated in a hiPSCMs-based in-vitro model. Additionally, the miRNA-transcription factor-gene regulatory revealed several top-ranked transcription factors including Tcf12, Ctcf, Spdef, Ebf1, Sp1, Rcor1 and miRNAs including miR-124-3p, miR-195-5p, miR-146a-5p, miR-17-5p, miR-15b-5p, miR-424-5p which may be involved in the regulation of genes associated with AIC.

Conclusions

Collectively, the current study suggested the important role of the key genes, oxytocin signaling pathway, and the miRNA-transcription factor-gene regulatory network in elucidating the molecular mechanism of AIC.
Associations between polymorphisms of SLC22A7, NGFR, ARNTL and PPP2R2B genes and Milk production traits in Chinese Holstein
Authors: Jia Ruike , Fu Yihan , Xu Lingna , Li Houcheng , Li Yanhua , Liu Lin , Ma Zhu , Sun Dongxiao , Han Bo
Published: November 2021

Background

Our preliminary work confirmed that, SLC22A7 (solute carrier family 22 member 7), NGFR (nerve growth factor receptor), ARNTL (aryl hydrocarbon receptor nuclear translocator like) and PPP2R2B (protein phosphatase 2 regulatory subunit Bβ) genes were differentially expressed in dairy cows during different stages of lactation, and involved in the lipid metabolism through insulin, PI3K-Akt, MAPK, AMPK, mTOR, and PPAR signaling pathways, so we considered these four genes as the candidates affecting milk production traits. In this study, we detected polymorphisms of the four genes and verified their genetic effects on milk yield and composition traits in a Chinese Holstein cow population.

Results

By resequencing the whole coding region and part of the flanking region of SLC22A7, NGFR, ARNTL and PPP2R2B, we totally found 20 SNPs, of which five were located in SLC22A7, eight in NGFR, three in ARNTL, and four in PPP2R2B. Using Haploview4.2, we found three haplotype blocks including five SNPs in SLC22A7, eight in NGFR and three in ARNTL. Single-SNP association analysis showed that 19 out of 20 SNPs were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage in the first and second lactations (P < 0.05). Haplotype-based association analysis showed that the three haplotypes were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage (P < 0.05). Further, we used SOPMA software to predict a SNP, 19:g.37095131C > T in NGFR, changed the structure of NGFR protein. In addition, we used Jaspar software to found that four SNPs, 19:g.37113872C > G,19:g.37113157C > T, and 19:g.37112276C > T in NGFR and 15:g.39320936A > G in ARNTL, could change the transcription factor binding sites and might affect the expression of the corresponding genes. These five SNPs might be the potential functional mutations for milk production traits in dairy cattle.

Conclusions

In summary, we proved that SLC22A7, NGFR, ARNTL and PPP2R2B have significant genetic effects on milk production traits. The valuable SNPs can be used as candidate genetic markers for genomic selection of dairy cattle, and the effects of these SNPs on other traits need to be further verified.
Genome-wide investigation of the GRAS transcription factor family in foxtail millet (Setaria italica L.)
Authors: Fan Yu , Wei Xiaobao , Lai Dili , Yang Hao , Feng Liang , Li Long , Niu Kexin , Chen Long , Xiang Dabing , Ruan Jingjun , Yan Jun , Cheng Jianping
Published: November 2021

Background

GRAS transcription factors perform indispensable functions in various biological processes, such as plant growth, fruit development, and biotic and abiotic stress responses. The development of whole-genome sequencing has allowed theGRASgene family to be identified and characterized in many species. However, thorough in-depth identification or systematic analysis ofGRASfamily genes in foxtail millet has not been conducted.

Results

In this study, 57GRASgenes of foxtail millet (SiGRASs) were identified and renamed according to the chromosomal distribution of theSiGRASgenes. Based on the number of conserved domains and gene structure, theSiGRASgenes were divided into 13 subfamilies via phylogenetic tree analysis. TheGRASgenes were unevenly distributed on nine chromosomes, and members of the same subfamily had similar gene structures and motif compositions. Genetic structure analysis showed that mostSiGRASgenes lacked introns. SomeSiGRASgenes were derived from gene duplication events, and segmental duplications may have contributed more toGRASgene family expansion than tandem duplications. Quantitative polymerase chain reaction showed significant differences in the expression ofSiGRASgenes in different tissues and stages of fruits development, which indicated the complexity of the physiological functions ofSiGRAS. In addition, exogenous paclobutrazol treatment significantly altered the transcription levels of DELLA subfamily members, downregulated the gibberellin content, and decreased the plant height of foxtail millet, while it increased the fruit weight. In addition,SiGRAS13andSiGRAS25may have the potential for genetic improvement and functional gene research in foxtail millet.

Conclusions

Collectively, this study will be helpful for further analysing the biological function ofSiGRAS. Our results may contribute to improving the genetic breeding of foxtail millet.
The role of JrLACs in the lignification of walnut endocarp
Authors: Li Pingping , Wang Hongxia , Liu Pan , Li Yaoling , Liu Kai , An Xiuhong , Zhang Zhihua , Zhao Shugang
Published: November 2021

Background

The walnut shell, which is composed of a large number of sclereids originating from the lignified parenchyma of the endocarp, plays an important role in fruit development and during harvesting and storage. The physical and chemical properties of walnut shells are closely related to the lignin content. Laccase is the key enzyme responsible for lignin biosynthesis by the polymerization of monolignols and plays crucial roles in secondary cell wall formation in plants. In this study, we screened and identified laccase family genes from the walnut genome and investigated the expression of laccase during endocarp lignification in walnut.

Results

A total of 37 laccase genes were screened from the walnut genome and distributed on nine chromosomes and classified into 6 subfamilies, among which subfamily IV showed distinct expansion. We observed that endocarp lignification started 44 days after flowering (DAF), and at later periods, the lignin content increased rapidly, with growth peaks at 44–50 DAF and 100–115 DAF. The lignification of the endocarp proceeded from the outside to the inside, as demonstrated by section staining in combination with endocarp staining. Furthermore, the changes in the expression of laccase family genes in the endocarp at different developmental stages were studied, and JrLACs showed different expression trends. The expression of nine genes showed significant increase after 44 DAF, and among these, JrLAC12–1, JrLAC12–2 and JrLAC16 showed a significant change in expression at the lignification stage. A study of the expression of JrLACs in different tissues and at various endocarp developmental stages revealed, that most JrLACs were expressed at low levels in mature tissues and at high levels in young tissues, in particular, JrLAC12–1 showed high expression in the young stems. A significant positive correlation was found between the expression of JrLAC12–1 and the variation in the lignin content in the endocarp.

Conclusion

Laccase genes play an important role in the lignification of the walnut endocarp, and JrLACs play different roles during fruit development. This study shows that JrLAC12–1 may play a key role in the lignification of endocarp.
Three case reports of West Nile virus neuroinvasive disease: lessons from real-life clinical practice
Authors: Falcinella Camilla , Allegrini Marina , Gazzola Lidia , Mulè Giovanni , Tomasoni Daniele , Viganò Ottavia , d’Arminio Monforte Antonella , Marchetti Giulia , Tincati Camilla
Published: November 2021

Background

Despite being an uncommon cause of meningoencephalitis, West Nile virus (WNV) recently provoked significant outbreaks throughout Europe. West Nile neuroinvasive disease (WNND) is associated with significant morbidity and mortality in older and compromised individuals, while its diagnosis may be demanding for the clinician. Here discussed are three cases of WNND with a focus on the diagnostic challenges they presented due to atypical clinical presentation and laboratory findings.

Case presentation

Between July and September 2020 three patients presented to our attention with signs and symptoms compatible with meningoencephalitis. Among routine procedures, they underwent lumbar puncture and imaging. In the absence of microbiological isolates, biological samples were sent for serology and NAATs for WNV. Following diagnosis, the patients gradually recovered and were discharged either home or to rehabilitation facilities.

Conclusions

The laboratory findings here discussed, in particular CSF parameters, are only partially consistent with those described in the literature, which highlights the need for further research. While serology and NAATs on blood and urine appear the most reliable techniques in the diagnostic work-up of WNND, utility of NAATs on CSF specimens is limited by the kinetics of WNV viremia in biological fluids. This report underlines that WNND should always be included in the differential diagnosis of meningoencephalitis during WNV transmission period.
Respiratory symptoms as initial manifestations of interstitial lung disease in clinically amyopathic juvenile dermatomyositis: a case report with literature review
Authors: Xia Jingyi , Jiang Gaoli , Jin Tingting , Shen Quanli , Ma Yangyang , Wang Libo , Qian Liling
Published: November 2021

Background

Clinically amyopathic juvenile dermatomyositis (CAJDM) is a clinical subgroup of juvenile dermatomyositis (JDM), characterized by JDM rashes with little or no clinically evident muscle weakness. Interstitial lung disease (ILD) is an uncommon but potentially fatal complication of juvenile dermatomyositis (JDM). While adults with dermatomyositis-associated ILD usually present respiratory symptoms before or at the same time as skin muscle manifestations, only a few studies have covered the onset of respiratory symptoms of ILD in JDM patients, especially CAJDM. There is currently no clear effective treatment regime or any prognostic factors for CAJDM-associated ILD.

Case presentation

Here, we report the first case of a CAJDM patient who presented with respiratory symptoms as the initial manifestation. A 10-year-old male patient presented to the hospital with a complaint of progressive cough and chest pain. Violaceous macule and papules appeared a few days later and he was positive for anti-Ro-52 antibodies. Imaging showed diffuse interstitial infiltration in both lungs and lung function tests showed restrictive and obstructive ventilatory dysfunction. Muscular abnormalities were excluded by thigh magnetic resonance imaging (MRI) and electromyography. Skin biopsy showed pathognomonic findings consistent with DM. Lung biopsy indicated chronic inflammation of the mucosa. This patient was finally diagnosed with CAJDM complicated by ILD and prescribed methylprednisolone, immunoglobulin, prednisolone and mycophenolate mofetil (MMF) for treatment. The patient’s cutaneous and respiratory manifestations were largely improved. We retrospectively reviewed this and another six cases with CAJDM-associated ILD reported previously to better understand its clinical characteristics and effective management.

Conclusions

Initial respiratory symptoms with rapid progression in patients presenting Gottron papules should be considered manifestations of CAJDM-associated ILD. We also found a combination of corticosteroids, IVIG and MMF to be an effective method of arresting the progress of CAJDM-associated ILD and improving the prognosis of the patients.
Translation, adaptation and testing of an emergency care satisfaction scale in Swedish pediatric emergency departments
Authors: Wennick Anne , Schoug Dorota , Ekwall Anna , Axelsson Malin
Published: November 2021

Background

Pediatric healthcare today shows a rising demand for research focusing on children’s perspectives on and consumer satisfaction with the nursing care they receive. Therefore, the purpose of this study was to translate and adapt the Consumer Emergency Care Satisfaction Scale (CECSS), a paper-based, self-administered 19-item questionnaire originally developed in the United States and targeted towards adults, and then test the new version in Swedish pediatric emergency departments.

Methods

The study was designed with a two-phase approach. Firstly, a forward–backward translation of the CECSS, involving expert consensus, was performed, and then the questionnaire was adapted for children aged 10–18 and assessed for face and content validity. Secondly, the translated and adapted questionnaire was tested with a clinical sample for construct validity, internal consistency, and reliability. This last aspect was assessed using a structured telephone interview 7–10 days after the participant visited a pediatric emergency department. All children participating in this study gave their assent (< 15 years) or consent (≥ 15 years), and their guardian’s written informed consent was also obtained.

Results

The paper-based, self-administered 19-item Swedish version of the CECSS was tested on a clinical sample consisting of 203 nonurgent children (boys: n = 109, 53.7 % and girls: n = 94, 46.3 %) between 10 and 18 years (mean age 13.8, SD 2.29). The factor analysis revealed three factors that explain 63.1 % of the total variation in the 15 items. The Cronbach’s alphas for the three dimensions (caring,teaching, andclinical competence) varied between 0.79 and 0.88. The intraclass correlation coefficient (ICC) for the entire Swedish version of the CECSS was 0.58, and the ICCs for the three dimensions varied between 0.56 and 0.71.

Conclusions

The results show that the developed Swedish Pediatric Consumer Emergency Care Satisfaction Scale (p-CECSS-S) is a valid, stable and easy-to-use-questionnaire that can be used to assess children’s satisfaction with nursing care.
The effects of exposure to HIV in neonates at a referral hospital in South Africa
Authors: Mellqvist Helena , Saggers Robin T. , Elfvin Anders , Hentz Elisabet , Ballot Daynia E.
Published: November 2021

Background

Fewer infants are infected with HIV through mother-to-child transmission, making HIV-exposed but uninfected (HEU) infants a growing population. HIV-exposure seems to affect immunology, early growth and development, and is associated with higher morbidity and mortality rates. Currently, there is a lack of information regarding the clinical effects of HIV-exposure during the neonatal period.

Objectives

To identify a possible difference in mortality and common neonatal morbidities in HEU neonates compared to HIV-unexposed neonates.

Methods

This was a retrospective, descriptive study of all neonates admitted to the neonatal unit at Charlotte Maxeke Johannesburg Academic Hospital between 1 January 2017 and 31 December 2018. HEU neonates were compared to HIV-unexposed neonates.

Results

There were 3236 neonates included, where 855 neonates were HEU. The HEU neonates had significantly lower birth weight and gestational age. The HEU neonates had higher rates of neonatal sepsis (19.8% vs 14.2%, OR 1.49, p <  0.001), specifically for late onset sepsis, and required more respiratory support. NCPAP and invasive ventilation was more common in the HEU group (36.3% vs 31.3% required NCPAP, p = 0.008, and 20.1% vs 15,0% required invasive ventilation, p <  0.001). Chronic lung disease was more common among HIV-exposed neonates (12.2% vs 8.7%, OR 1.46, p = 0.003). The difference in mortality rates between the study groups was not significant (10.8% of HEU neonates and 13.3% of HIV-unexposed).

Conclusions

HEU neonates had higher rates of neonatal sepsis, particularly late-onset sepsis, required more respiratory support and had higher rates of chronic lung disease. Mortality of HEU neonates was not different HIV-unexposed neonates.
Pregnancy- and lactation-associated osteoporosis with vertebral fractures: a systematic review
Authors: Qian Ying , Wang Lei , Yu Lili , Huang Weimin
Published: November 2021

Background

To review, analyze and characterize the pregnancy and lactation-related osteoporosis (PLO) with vertebral fractures based on the extraction data in the previous studies.

Methods

A comprehensive literature search of electronic databases including the PubMed, Embase and Web of Science was conducted from January 1st,1990 to December 1st, 2020. The enrolled data were pooled to analyze the baseline characteristics, clinical features, risk factors and treatment options.

Results

A total of 65 articles with 338 cases were enrolled for data extraction. The enrolled cases aged from 19 to 47 years, with a mean value of 35.7 years old. The average body mass index (BMI) was 22.2 kg/m2 ranged from 16.0 to 39.0 kg/m2. Of the 173 cases, 149 cases with vertebral fractures occurred in the first pregnancy, 19 cases in the second pregnancy, four cases in the third pregnancy and one case in the fourth pregnancy. Up to 91.5% of the back pain occurred within the last 3 months of pregnancy and the first 3 months after delivery. The most involved vertebral levels were L2, L1 and T12 accounting for 32.6% of all the fractures. The average fracture numbers were 4.4 levels per patient. The lumbar Z-scores were mostly recorded with a mean value of − 3.2 ranged from − 7.8 to 0.

Conclusions

PLO with vertebral fractures is a rare clinical entity, which is more likely to occur in older and thinner pregnant women. Back pain is the clinical complaint and mostly occurs in the late pregnancy and early lactation periods. Most vertebral fractures appear in the first pregnancy but it can occur in any time of pregnancy. Thoracolumbar region is the mostly involved region. As compared with postmenopausal osteoporotic fractures, PLO usually has multiple levels fractures. Bisphosphonates are the most widely used treatment so far, however, many factors need to be taken into account to decide which drug to choose in PLO and further studies are necessary for clear recommendation in the future.
Psychological well-being and death anxiety among breast cancer survivors during the Covid-19 pandemic: the mediating role of self-compassion
Authors: Yousefi Afrashteh Majid , Masoumi Samin
Published: November 2021

Background

Despite the abundance of clinical data available for Coronavirus Disease 2019 (COVID-19), little research on the psychological well-being of breast cancer survivors has been published. We investigate the extent to which self-compassion accounted for the association between psychological well-being (depression, anxiety) and death anxiety in breast cancer survivors.

Methods

A cross-sectional study design was applied. Participants were recruited from three departments of oncology in Zanjan, Iran. Data were collected from 210 breast cancer patients. Participants completed self-report measures. Pearson correlation coefficient was used to assess the relationship among the study variables. Bootstrapping analyses were used to test the significance of indirect effects.

Results

Correlational analyses revealed that depression and anxiety were significantly and positively related to death anxiety (r = 0.77, p < 0.01; r = 0.85, p < 0.01, respectively) and negatively to self-compassion (r = − 0.48, p < 0.01; r = − 0.53, p < 0.01, respectively). Bootstrapping analyses revealed significant indirect effects of depression (β = 0.065, SE = 0.35, p < 0.03, 95% CI [LL = − 0.0083, UL: − 0.1654]) and anxiety (β = 0.089, SE = 0.09, p < 0.04, 95% CI [LL = − 0.0247, UL: − 0.1987]) on death anxiety through self-compassion.

Conclusions

Findings from this study indicate that self-compassion may be considered as one treatment strategy to improve psychological well-being of cancer patients in the new context of the COVID-19 pandemic.
Efficacy of artesunate-amodiaquine and artemether-lumefantrine for uncomplicated Plasmodium falciparum malaria in Madagascar, 2018
Authors: Dentinger Catherine M. , Rakotomanga Tovonahary Angelo , Rakotondrandriana Antsa , Rakotoarisoa Arinomenjanahary , Rason Marie Ange , Moriarty Leah F. , Steinhardt Laura C. , Kapesa Laurent , Razafindrakoto Jocelyn , Svigel Samaly S. , Lucchi Naomi W. , Udhayakumar Venkatachalam , Halsey Eric S. , Ratsimbasoa C. Arsène
Published: November 2021

Background

Since 2005, artemisinin-based combination therapy (ACT) has been recommended to treat uncomplicated falciparum malaria in Madagascar. Artesunate-amodiaquine (ASAQ) and artemether-lumefantrine (AL) are the first- and second-line treatments, respectively. A therapeutic efficacy study was conducted to assess ACT efficacy and molecular markers of anti-malarial resistance.

Methods

Children aged six months to 14 years with uncomplicated falciparum malaria and a parasitaemia of 1000–100,000 parasites/µl determined by microscopy were enrolled from May–September 2018 in a 28-day in vivo trial using the 2009 World Health Organization protocol for monitoring anti-malarial efficacy. Participants from two communes, Ankazomborona (tropical, northwest) and Matanga (equatorial, southeast), were randomly assigned to ASAQ or AL arms at their respective sites. PCR correction was achieved by genotyping seven neutral microsatellites in paired pre- and post-treatment samples. Genotyping assays for molecular markers of resistance in the pfk13, pfcrt and pfmdr1 genes were conducted.

Results

Of 344 patients enrolled, 167/172 (97%) receiving ASAQ and 168/172 (98%) receiving AL completed the study. For ASAQ, the day-28 cumulative PCR-uncorrected efficacy was 100% (95% CI 100–100) and 95% (95% CI 91–100) for Ankazomborona and Matanga, respectively; for AL, it was 99% (95% CI 97–100) in Ankazomborona and 83% (95% CI 76–92) in Matanga. The day-28 cumulative PCR-corrected efficacy for ASAQ was 100% (95% CI 100–100) and 98% (95% CI 95–100) for Ankazomborona and Matanga, respectively; for AL, it was 100% (95% CI 99–100) in Ankazomborona and 95% (95% CI 91–100) in Matanga. Of 83 successfully sequenced samples for pfk13, no mutation associated with artemisinin resistance was observed. A majority of successfully sequenced samples for pfmdr1 carried either the NFD or NYD haplotypes corresponding to codons 86, 184 and 1246. Of 82 successfully sequenced samples for pfcrt, all were wild type at codons 72–76.

Conclusion

PCR-corrected analysis indicated that ASAQ and AL have therapeutic efficacies above the 90% WHO acceptable cut-off. No genetic evidence of resistance to artemisinin was observed, which is consistent with the clinical outcome data. However, the most common pfmdr1 haplotypes were NYD and NFD, previously associated with tolerance to lumefantrine.