bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental and Theoretical Studies of the Vibrational and Electronic Properties of (2E)-2-[3-(1H-imidazol-1-yl)-1-phenyl-propylidene]-N-phenylhydrazinecarboxamide: An Anticonvulsant Agent
Authors: Govindarajan Munusamy , Abdelhameed Ali , Al-Saadi Abdulaziz , Attia Mohamed
Published: October 2015

In the current investigation, the molecular structure of the anticonvulsant agent (2E)-2-[3-(1H-imidazol-1-yl)-1-phenylpropylidene]-N-phenylhydrazinecarboxamide ((2E)-HIPC) was theoretically modelled using ab initio Hartree-Fock (HF) and density functional theory (DFT/B3LYP) calculations. The Fourier transform (FT) infrared and FT-Raman spectra of (2E)-HIPC were also recorded, and the observed bands were assigned to the vibrational normal modes. The main functional groups were identified via vibrational analysis, and their absorption bands were assigned. A comparative analysis was performed for the computed and experimental results. Subtle differences were observed between the calculated and experimental UV-Vis spectra. Time-dependent density functional theory (TD-DFT) excitation energies were calculated for five excited electronic states. The calculations were applied to simulate the spectra of (2E)-HIPC, and these simulated spectra exhibited excellent agreement with the experimental spectra. The DFT/B3LYP/6-311++G(d,p) method, after scaling, exhibited better agreement with the experimental values than the results obtained by the HF method. The energy, oscillator strength, and wavelength computed by TD-DFT (IEFPCM) are consistent with the experimental results. The molecular electrostatic potential (MEP) and frontier molecular orbitals (HOMO-LUMO) were also determined to enable prediction of the structural changes and reactive sites. Mulliken population charges of the title molecule were also calculated in the gas phase. The NMR chemical shifts (13C and 1H) were calculated using the gauge-including atomic orbital method and the B3LYP/6-311++G(d,p) approach and were compared with the experimental values.
A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy
Authors: Haubner Frank , Muschter Dominique , Pohl Fabian , Schreml Stephan , Prantl Lukas , Gassner Holger
Published: October 2015

External radiation seems to be associated with increased amounts of cytokines and other cellular modulators. Impaired microcirculation and fibrosis are examples of typical long term damage caused by radiotherapy. Adipose tissue-derived stem cells (ASC) are discussed to enhance wound healing, but their role in wounds due to radiotherapy is poorly understood. Normal human fibroblasts (NHF) and ASCs were co-cultured and external radiation with doses from 2–12 Gray (Gy) was delivered. Cell proliferation and mRNA levels of matrix metalloproteinases (MMP1, MMP2 and MMP13) were determined 48 h after irradiation of the co-cultures by qPCR. Additionally, tissue inhibitors of matrix metalloproteinases (TIMP1, TIMP2) were determined by enzyme-linked immunosorbent assay (ELISA). There was a reduction of cell proliferation after external radiation in mono-cultures of NHFs and ASCs compared to controls without irradiation. The co-culture of ASCs and NHFs showed reduced impairment of cell proliferation after external radiation. Gene expression of MMP1 and MMP13 was reduced after external irradiation in NHF. MMP2 expression of irradiated NHFs was increased. In the co-culture setting, MMP1 and MMP2 gene expression levels were upregulated. TIMP1 and TIMP2 protein expression was increased after irradiation in NHFs and their co-cultures with ASCs. ASCs seem to stimulate cell proliferation of NHFs and modulate relevant soluble mediators as well as proteinases after external radiation.
The Functions of BMP3 in Rabbit Articular Cartilage Repair
Authors: Zhang Zhe , Yang Wenyu , Cao Yiting , Shi Yanping , Lei Chen , Du Bo , Li Xuemin , Zhang Qiqing
Published: October 2015

Bone morphogenetic proteins (BMPs) play important roles in skeletal development and repair. Previously, we found fibroblast growth factor 2 (FGF2) induced up-regulation of BMP2, 3, 4 in the process of rabbit articular cartilage repair, which resulted in satisfactory repair effects. As BMP2/4 show a clearly positive effect for cartilage repair, we investigated the functions of BMP3 in rabbit articular cartilage repair. In this paper, we find that BMP3 inhibits the repair of partial-thickness defect of articular cartilage in rabbit by inducing the degradation of extracellular matrix, interfering with the survival of chondrocytes surrounding the defect, and directly inhibiting the expression of BMP2 and BMP4. Meanwhile BMP3 suppress the repair of full-thickness cartilage defect by destroying the subchondral bone through modulating the proliferation and differentiation of bone marrow stem cells (BMSCs), and directly increasing the expression of BMP4. Although BMP3 has different functions in the repair of partial and full-thickness defects of articular cartilage in rabbit, the regulation of BMP expression is involved in both of them. Together with our previous findings, we suggest the regulation of the BMP signaling pathway by BMP3 is essential in articular cartilage repair.
Batch Attribute-Based Encryption for Secure Clouds
Authors: Yang Chen , Sun Yang , Wu Qianhong
Published: October 2015

Cloud storage is widely used by organizations due to its advantage of allowing universal access with low cost. Attribute-based encryption (ABE) is a kind of public key encryption suitable for cloud storage. The secret key of each user and the ciphertext are associated with an access policy and an attribute set, respectively; in addition to holding a secret key, one can decrypt a ciphertext only if the associated attributes match the predetermined access policy, which allows one to enforce fine-grained access control on outsourced files. One issue in existing ABE schemes is that they are designed for the users of a single organization. When one wants to share the data with the users of different organizations, the owner needs to encrypt the messages to the receivers of one organization and then repeats this process for another organization. This situation is deteriorated with more and more mobile devices using cloud services, as the ABE encryption process is time consuming and may exhaust the power supplies of the mobile devices quickly. In this paper, we propose a batch attribute-based encryption (BABE) approach to address this problem in a provably-secure way. With our approach, the data owner can outsource data in batches to the users of different organizations simultaneously. The data owner is allowed to decide the receiving organizations and the attributes required for decryption. Theoretical and experimental analyses show that our approach is more efficient than traditional encryption implementations in computation and communication.
3-(3,4-Dihydroxyphenyl)-8-hydroxy-2H-chromen-2-one
Authors: Svinyarov Ivan , Bogdanov Milen
Published: October 2015

We report a novel protocol for the synthesis of 3-(3,4-dihydroxyphenyl)-8-hydroxy-2H-chromen-2-one via demethylation/delactonization/elimination/lactonization/ decarboxylation domino reaction sequence of diastereomeric mixture of cis- and trans-3-(2,3-dimethoxyphenyl)-6,7-dimethoxy-1-oxoisochroman-4-carboxylic acids in boiling HBr/AcOH. The structure of the target compound was established for the first time by means of spectral methods such as 1H-, 13C-, DEPT-135-NMR, IR and HRMS.
An Approach to the Integrated Design of PCM-Air Heat Exchangers Based on Numerical Simulation: A Solar Cooling Case Study
Authors: Dolado Pablo , Lazaro Ana , Delgado Monica , Peñalosa Conchita , Mazo Javier , Marin Jose , Zalba Belen
Published: October 2015

A novel technique of design of experiments applied to numerical simulations is proposed in this paper as a methodology for the sizing and design of thermal storage equipment integrated in any specific application. The technique is carried out through the response surfaces in order to limit the number of simulation runs required to achieve an appropriate solution. Thus, there are significant savings on the time spent on the design as well as a potential cost saving on the experimentation if similarity relationships between the prototype and the model are met. The technique is applied here to a previously developed and validated numerical model that simulates the thermal behavior of a phase change material-air heat exchanger. The incorporation of the thermal energy storage unit is analyzed in the case of a solar cooling application, improving the system coefficient of performance. The economic viability is mainly conditioned by the price of the macroencapsulated phase change material.
It’s Not Me, It’s You: The Disconnect of Physical Education Teachers to Physical Activity in the Gym
Authors: Culpepper Dean , Killion Lorraine
Published: October 2015

American adults and an increasing number of children, are not meeting the recommended amounts of daily physical activity. Research has documented the effects of low activity on health and increasingly new research has shown that low activity levels now impact academic achievement. Physical education (PE) can play an important role if the children participating are obtaining enough physical activity while in class and the PE program has not been targeted with cutbacks. The purpose of this study was to identify the amount and intensity of activity in PE classes. The results indicate that on average students at all levels are not meeting the activity requirements. In addition, PE teachers’ perceptions of physical activity in their own class does not match actual activity levels. PE teachers must do a better job of increasing activity levels in their classrooms. Mounting research indicates students perform better academically if they are physically active. PE is the only subject where teachers can organize activities that meet both activity and intensity requirements.
The Use of Synthetic Carriers in Malaria Vaccine Design
Authors: Powles Liam , Xiang Sue , Selomulya Cordelia , Plebanski Magdalena
Published: October 2015

Malaria vaccine research has been ongoing since the 1980s with limited success. However, recent improvements in our understanding of the immune responses required to combat each stage of infection will allow for intelligent design of both antigens and their associated delivery vaccine vehicles/vectors. Synthetic carriers (also known as vectors) are usually particulate and have multiple properties, which can be varied to control how an associated vaccine interacts with the host, and consequently how the immune response develops. This review comprehensively analyzes both historical and recent studies in which synthetic carriers are used to deliver malaria vaccines. Furthermore, the requirements for a synthetic carrier, such as size, charge, and surface chemistry are reviewed in order to understand the design of effective particle-based vaccines against malaria, as well as providing general insights. Synthetic carriers have the ability to alter and direct the immune response, and a better control of particle properties will facilitate improved vaccine design in the near future.
Incidence of complications associated with tibial tuberosity advancement in Boxer dogs
Authors: Sul Rui , Parkin Tim , Calvo Ignacio , de Lima Dantas Brigite
Published: October 2015

Objective: To retrospectively review and describe the incidence of complications associated with tibial tuberosity advancement (TTA) surgical procedures in a group of Boxer dogs (n = 36 stifles) and compare the data with a non-Boxer control population (n = 271 stifles).

Methods: Retrospective analysis of medical records to identify all dogs that underwent TTA surgery due to cranial cruciate ligament disease. These records were categorized into two groups: Boxer dogs and non-Boxer dogs (controls – all other breeds).

Results: Of the 307 stifles included, 69 complications were reported in 58 joints. The complication rate differed significantly for Boxer dogs (16/36 stifles) and non-Boxer dogs (42/271 stifles), corresponding to an odds ratio of 5.8 (confidence interval: 1.96–17.02; p-value <0.001). Boxer dogs were more likely to undergo revision surgery and to develop multiple complications. The incidence of tibial tuberosity fractures requiring surgical repair (2/36 versus 1/271) and incisional infections requiring antibiotic treatment (three in each group) was significantly higher in the Boxer group.

Clinical significance: Boxer dogs had more major and multiple complications after TTA surgery than the control non-Boxer group; these complications included higher rates of revision surgery, tibial tuberosity fractures requiring stabilization, and infection related complications. The pertinence and value of breed-specific recommendations for cranial cruciate ligament disease appears to be a subject worthy of further investigation.
Adipose Tissue-Derived Mesenchymal Stem Cells Induce Expansion of Interleukin-10-Producing Regulatory B Cells and Ameliorate Autoimmunity in a Murine Model of Systemic Lupus Erythematosus
Authors: Park Min-Jung , Kwok Seung-Ki , Lee Sung-Hee , Kim Eun-Kyung , Park Sung-Hwan , Cho Mi-La
Published: October 2015

Mesenchymal stem cells (MSCs) are multipotent cells characterized by immunomodulatory properties and are therefore considered a promising tool for the treatment of autoimmune diseases. One functional B-cell subset, regulatory B cells (Bregs), has recently been shown to restrain excessive inflammatory responses in autoimmune diseases. In the present study, we investigated the impact of human adipose-derived MSCs on Bregs and their therapeutic effect in an animal model of systemic lupus erythematosus (SLE). Coculture of human adipose-derived MSCs with splenocytes from C57BL/6 mice expanded the population of interleukin-10-producing B cells (B10 B cells). In vivo treatment with human adipose-derived MSCs reduced serum anti-double-stranded antibody levels and improved renal pathology of lupus mice (Roquinsan/sanmice). MSCs decreased ICOS+CD44+follicular helper T cells, Th1 cells and Th17 cells, in spleens of Roquinsan/sanmice. In contrast, MSCs increased Foxp3-expressing regulatory T cells. MSCs also decreased the size and number of germinal centers and effector B cells. As expected, in vivo treatment with MSCs expanded the population of Bregs in spleens of Roquinsan/sanmice. Our results indicate that human adipose-derived MSCs induce the expansion of Bregs and ameliorate autoimmunity in a murine model of SLE. These findings suggest that human adipose-derived MSCs may be a promising therapeutic strategy targeting B-cell-mediated autoimmune diseases such as SLE.
Longitudinal Evaluation of Effects of Intra-Articular Mesenchymal Stromal Cell Administration for the Treatment of Osteoarthritis in an Ovine Model
Authors: Delling Uta , Brehm Walter , Ludewig Eberhard , Winter Karsten , Jülke Henriette
Published: October 2015

In this study, the therapeutic effect of intra-articularly injected autologous mesenchymal stromal cells (MSCs) was evaluated in an ovine osteoarthritis (OA) model using consecutive magnetic resonance imaging (MRI), radiography, and macroscopic and histologic postmortem examination. In 12 sheep, OA was induced by bilateral, lateral meniscectomy. After 6 weeks, 20 × 106bone marrow-derived MSCs (50% MSCs were superparamagnetic iron oxide particle labeled) were injected intra-articularly into one knee joint. The contralateral knee served as negative control. MR images were acquired before OA induction, immediately before and after MSC injection, and 1, 4, 8, and 12 weeks thereafter using a 0.5T unit and a T2* gradient echo sequence. Radiographs were obtained before OA induction, at MSC injection, and 12 weeks thereafter. The MRI scoring system included articular cartilage, bone, joint capsule, and synovial fluid evaluation. The radiographic scoring system included the joint space and bone. Postmortem evaluation entailed macroscopic and histologic assessment. Longitudinal MRI revealed a significant deceleration of OA progression in MSC-treated joints. However, at the conclusion of the study, there was no significant difference in the degree of OA detected by MRI, radiography, and postmortem evaluation between the treatment and control group. The degree of OA on MRI varied among the 12 animals at the time of injection, but there was no difference between the left and right limb. In conclusion, intra-articular MSCs decreased OA progression. However, no significant treatment effects were seen at the conclusion of the study at 12 weeks. This somewhat contradicts previously published results. Nevertheless, the choice of OA model, outcome measures, or lack of additional medication might explain the differences. Our results indicate that OA might benefit from intra-articular MSC injection, but further studies are needed to refine patient selection and injection parameters for a more substantially improved outcome.
Regeneration of Degenerated Urinary Sphincter Muscles: Improved Stem Cell-Based Therapies and Novel Imaging Technologies
Authors: Amend Bastian , Vaegler Martin , Fuchs Kerstin , Mannheim Julia G. , Will Susanne , Kramer Ulrich , Hart Melanie L. , Feitz Wouter , Chapple Christopher , Stenzl Arnulf , Aicher Wilhelm K.
Published: October 2015

Stress urinary incontinence (SUI) is a largely ousted but significant medical, social, and economic problem. Surveys suggest that nowadays approximately 10% of the male and 15% of the female population suffer from urinary incontinence at some stage in their lifetime. In women, two major etiologies contribute to SUI: degeneration of the urethral sphincter muscle controlling the closing mechanism of the bladder outflow and changes in lower pelvic organ position associated with degeneration of connective tissue or with mechanical stress, including obesity and load and tissue injury during pregnancy and delivery. In males, the reduction of the sphincter muscle function is sometimes due to surgical interventions as a consequence of prostate cancer treatment, benign prostate hyperplasia, or of neuropathical origin. Accordingly, for women and men different therapies were developed. In some cases, SUI can be treated by physical exercise, electrophysiological stimulation, and pharmacological interventions. If this fails to improve the situation, surgical interventions are required. In standard procedures, endoprostheses for mechanical support of the weakened tissue or mechanical valves for a bladder outflow control are implanted. In 20% of cases treated, repeat procedures are required as implants yield all sorts of side effects in time. Based on preclinical studies, the application of an advanced therapy medicinal product (ATMP) such as implantation of autologous cells may be a curative and long-lasting therapy for SUI. Cellular therapy could also be an option for men suffering from incontinence caused by injury of the nerves controlling the muscular sphincter system. Here we briefly report on human progenitor cells, especially on mesenchymal stromal cells (MSCs), their expansion and differentiation to smooth muscle or striated muscle cells in vitro, labeling of cells for in vivo imaging, concepts of improved, precise, yet gentle application of cells in muscle tissue, and monitoring of injected cells in situ.
Effects of Destination Image and Total Perceived Value on Tourists' Behavioral Intentions: An Investigation of Domestic Festival Tourists
Authors: Lban Mehmet Oğuzhan , Kaşli Mehmet , Bezirgan Muammer
Published: October 2015

The aim of this study is to identify the relationship between the destination image perceived by domestic festival tourists visiting Burhaniye and their perceived values (PV) and behavioral intentions (BI). A research model was created to determine the relationships between variables
in an equation model. The research findings showed that destination image (DI) positively affects the value of the perception of a destination and word-of-mouth communication. However, destination image had no effect on the intention to revisit. The value perceived from a destination positively
affected the likelihood of revisiting and suggesting the destination to others. The results of the study are discussed, and limitations and suggestions related to the study are presented.
Academic Insulae: The Search for a Paradigm in and for Tourism Studies
Authors: Platenkamp Vincent
Published: October 2015

Epistemological relativism in tourism studies has been conceivably paralyzed by the concept of a, or, the “paradigm.” In this review article, Platenkamp metaphorically identifies these paradigms with the islands that Odysseus visited (all those centuries ago) during his
well-recorded journey to Ithaca. In this context, therefore, Ithaca is changed (by Platenkamp) from being just an idyllic Greek homeland into a contemporary, hybridized world like—in our time—of the multilayered network society in Africa of the capital of Ghana, Kumasi. The basic
question for Platenkamp, then, is that of how tourism studies researchers can (or ought?) leave their safe islands (i.e., their paradigms) and organize their own paradigm dialog (after Guba) with others around them on their uncertain and risky voyage to Kumasi. In an attempt to clarify
this vital kind of dialog, Platenkamp introduces Said's principles of reception and resistance, but also focuses on the distinction between different modes of “knowledge production” that have been introduced into the social sciences since the 1990s. In this light, to Platenkamp,
the uncertainty of this ongoing/unending epistemological quest remains crucial: to him, all (almost all?) believers in a, or any, paradigm within tourism studies are unhealthily “overimmunized” by the tall claims and the perhaps undersuspected strategies of the particular “paradigm”
they follow.
A SIMPLIFIED MULTI-CRITERIA EVALUATION MODEL FOR LANDFILL SITE RANKING AND SELECTION BASED ON AHP AND GIS
Authors: KHAN Debishree , SAMADDER Sukha Ranjan
Published: October 2015

This study used GIS based Multi-criteria Decision Analysis (MCDA) approach for evaluating the most environmentally suitable landfill sites in the study area. The weights of relative importance of the factors guiding landfill siting were estimated using pair-wise comparisons in AHP. The maps showing suitable landfill sites were generated applying a weighted linear combination (WLC) in GIS using a comparison matrix to aggregate different significant scenarios associated with environmental and economic objectives. To determine the appropriate areas where landfill sites can be located, thematic maps for all the criteria were generated using GIS. A final map was produced showing suitability for the location of the landfill sites. The suitable sites having an area equal to or above 4 ha at one place and 90% of which is barren land were considered suitable for landfill. The selected candidate sites were ranked to get the most desirable sites for landfill.
Secondary hemophagocytic lymphohistiocytosis in children with brucellosis: report of three cases
Authors: Yaman Yöntem , Gözmen Salih , Özkaya Ahmet Kağan , Oymak Yeşim , Apa Hurşit , Vergin Canan , Devrim İlker
Published: October 2015

Brucellosis is a systemic zoonotic infectious disease that may cause fever, fatigue, sweating, arthritis, hepatosplenomegaly, cytopenia, and lymphadenopathy. It continues to be an important health problem worldwide. Hemophagocytic lymphohistiocytosis (HLH) is characterized by fever, hepatosplenomegaly, cytopenias, high serum levels of ferritin and triglycerides, low serum fibrinogen levels, and hemophagocytosis in bone marrow, lymph nodes, spleen, or liver. Hemophagocytic lymphohistiocytosis associated with brucellosis is a very rare condition in the pediatric age group. Here, three pediatric cases of secondary HLH associated with brucellosis are reported. Hemophagocytic lymphohistiocytosis should be considered in patients with brucellosis having cytopenias. Hemophagocytosis in brucellosis seems to be cured with appropriate antibiotics and intravenous immunoglobulin.
Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis
Authors: Ramis Ivy Bastos , Vianna Júlia Silveira , Halicki Priscila Cristina Bartolomeu , Lara Caroline , Tadiotto Thássia Fernanda , Da Silva Maciel João Batista , Gonçalves Carla Vitola , Von Groll Andrea , Dellagostin Odir Antônio , Da Silva Pedro Eduardo Almeida
Published: October 2015

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases. Methodology: Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR. Results: It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B.  No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found. Conclusions: We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.
A hospital based pilot study on Epstein-Barr virus in suspected infectious mononucleosis pediatric patients in India
Authors: Janani Madhuravasal Krishnan , Malathi Jambulingam , Appaswamy Andal , Singha Nishi Rani , Madhavan Hajib Nariharirao
Published: October 2015

Introduction: Infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV) is commonly diagnosed by detection of antibodies in the patient’s sera. Differentiation of acute from chronic and differential diagnosis of EBV-induced IM from IM-like syndrome caused by human cytomegalovirus (CMV) is important. The objective of this study was to standardize and use polymerase chain reaction (PCR) for diagnosis of EBV and evaluate it against enzyme-linked immunosorbent assay (ELISA). Methodology: ELISA for detection of IgM and IgG antibodies to viral capsid antigen (VCA) and PCR targeting the VCA and EBNA1 gene of EBV and mtrII gene of CMV were performed on180 peripheral blood samples collected from 180 patients with suspected IM. The analytical sensitivity of PCR was evaluated against that of ELISA. Results: Using the standard serological profile as the reference, the EBV-VCA gene was detected in 41 (95%) of 45 samples collected from patients with early primary infections, in 41 (54%) of 75 with recent primary infections, and in7 (17%) of 39 with past infections. The result of VCA PCR was statistically significant in virus detection during early or primary stage of infection. Nineteen (49%) EBV-seropositive samples were positive for CMV by PCR. All control samples tested negative for both VCA and EBNA1by PCR. Conclusions: VCA PCR is sensitive for the detection of EBV DNA in the early or primary stage of infection and can be considered a reliable method to rule out the cross-reactivity and differential diagnosis of EBV-induced IM from IM-like syndrome.
Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt
Authors: Aboushleib Hamida M. , Omar Hoda M. , Abozahra Rania , Elsheredy Amel , Baraka Kholoud
Published: October 2015

Introduction: Pseudomonas aeruginosa is one of the most virulent nosocomial pathogens worldwide. Quorum sensing (QS) regulates the production of pathogenic virulence factors and biofilm formation in P. aeruginosa. The four genes lasR, lasI, rhlR,and rhlI were found to regulate this QS system. In this study, we aimed to assess the correlation between these four genes and QS-dependent virulence factors and to detect the inhibitory effect of clove oil on QS. Methodology: Fifty P. aeruginosa clinical isolates were collected. Susceptibility to different antibiotics was tested. Virulence factors including biofilm formation, pyocyanin production, and twitching motility were phenotypically detected. QS genes were amplified by polymerase chain reaction (PCR), and one strain subsequently underwent sequencing. The inhibitory effect of clove oil on virulence factors was also tested. Results: A positive correlation was found between biofilm formation and the presence of lasR and rhlI genes. Twitching motility was positively correlated with the presence of lasR, lasI, and rhlI genes. On the other hand, no correlation was found between pyocyanin production and any of the studied genes. Only one isolate amplified all the tested QS gene primers, but it did not express any of the tested virulence factors phenotypically. Sequence analyses of this isolate showed that the four genes had point mutations. Conclusions: Results emphasize the importance of QS in P. aeruginosa virulence; however, QS-deficient clinical isolates occur and are still capable of causing clinical infections in humans. Also, clove oil has an obvious inhibitory effect on QS, which should be clinically exploited.
Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)
Authors: Belkahia Hanène , Ben Said Mourad , Sayahi Lotfi , Alberti Alberto , Messadi Lilia
Published: October 2015

Introduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels. Methodology: A total of 226 camels from three different bioclimatic areas were sampled and tested for the presence of Anaplasma species by quantitative polymerase chain reaction (qPCR) and nested polymerase chain reaction (nPCR) assays. Detected Anaplasma strains were characterized by 16S rRNA sequence analysis. Results: Overall infection rate of Anaplasma spp. was 17.7%, and was significantly higher in females. Notably, A. marginale, A. centrale, A. bovis, and A. phagocytophilum were not detected. Animals were severely infested by three tick species belonging to the genus Hyalomma (H. dromedarii, H. impeltatum, and H. excavatum). Alignment, similarity comparison, and phylogenetic analysis of the 16S rRNA sequence variants obtained in this study suggest that Tunisian dromedaries are infected by more than one novel Anaplasma strain genetically related to A. platys. Conclusions: This study reports the presence of novel Anaplasma sp. strains genetically related to A. platys in dromedaries from various bioclimatic areas of Tunisia. Findings raise new concerns about the specificity of the direct and indirect diagnostic tests routinely used to detect different Anaplasma species in ruminants and provide useful molecular information to elucidate the evolutionary history of bacterial species related to A. platys.
De l’intérêt des correspondances pour la sociologie et pour son histoire en particulier
Authors: Béra Matthieu
Published: October 2015

Cet article part d’un étonnement : le désintérêt des sociologues et des historiens de la sociologie pour les correspondances privées. Il voudrait inciter les sociologues à en faire au contraire un plus grand usage, pour des raisons méthodologiques et théoriques. Il montre que les trois grands « courants théoriques » (individualiste, interactionniste et holiste) peuvent, chacun, y retrouver un matériau en rapport avec leur optique respective. Ces documents privés donnent accès à une sociologie des individus, sensible aux rationalités individuelles, à l’exposé motivé de leurs choix, aussi bien qu’à leurs émotions. D’autre part, les échanges épistolaires objectivent les interactions sociales de leur naissance à leur clôture. Le social en train de se faire s’y donne à lire comme dans un livre ouvert et s’y révèle dans sa pureté, sans les artefacts liés à l’enquête. Enfin, les correspondances donnent un accès privilégié à l’échelle macro sociale, sous la forme des réseaux, institutions, voire des courants et des « écoles » qu’elles évoquent, ainsi que des éléments de contexte qu’elles révèlent.
L’essor de la science sociale à travers la correspondance de Paul de Rousiers à Robert Pinot (1891-1895)
Authors: Savoye Antoine
Published: October 2015

Cette présentation des lettres, au nombre de cent soixante, adressées par Paul de Rousiers à Robert Pinot, de 1891 à 1895, met l’accent sur les évènements majeurs intervenus au cours de cette période dont la correspondance rend compte : le succès rencontrée par La vie américaine , l’entrée au Musée social de R. Pinot et, surtout, les deux enquêtes menées par P. de Rousiers en Angleterre. Les lettres constituent ainsi un véritable journal de terrain de la science sociale en train de se faire en cette fin de siècle.
Sensor Network Deployment under Distance Uncertainty with Robust Optimization
Authors: Qiao Junfeng , Liu Sanyang , Zhang Jianke , Niu Yujun
Published: October 2015

We consider the sensor deployment problem in the context of distance uncertainty. It is characterized by differentiated arrangement of specific detection probability thresholds at different locations. The problem is formulated as an integer linear programming (ILP) model firstly, aiming at optimizing the number of sensors and their locations. Based on the robust discrete optimization methodology, the uncertain model is transformed into an equivalent ILP problem considering distance uncertainty. The proposed approach can control the tradeoff between optimality and robustness by varying the parameters named protection levels. Uniform and non-uniform event detection probabiliy distributions are considered in the experiment. The results show that, as the distance uncertainty increases, the constraint violation can be avoided in the robust model and the robust solution can provide a significant improvement at the expense of a small loss in optimality when compared to the optimal solution of a deterministic scenario.
Insect Trapping Method Based on Progressive Star Network
Authors: Qiu Zhongsheng , Qiu Pengfei , Zhu Qiankun , Wang Rong
Published: October 2015

This paper proposes an insect trapping method based on progressive star network. There are two steps: Firstly, construct the network; secondly, trap insects through the network. The network is constructed as following descriptions: 1. Confirm the density of light node. 2. Adjust the height of light node versus growth of crops/plants. This paper contributes to relevant studies in that it proposes a new star network, in which the height of light node changes according to the growth of crops or plants, makes full use of biological resources and avoids bad consequences that traditional chemical pesticides bring about. The star network proposed is simple, reliable and successfully avoids redundant data resulted from frequency communication
Multi-authority E-voting System Based on Group Blind Signature
Authors: Wang Haibin , Liu Xia , Zhao Sheng , Huo Lina
Published: October 2015

Targeting at some problems such as the control of a single authority and guarantee of privacy of multi-authority, this paper proposes a double-blind multi-authority e-voting system based Ghadafi’s group blind signature and SP-signature. By introducing multi-authority, this paper solves the control problem of a single authority. By using the SP-signature, voters cannot vote for others. The new system voter can protect confidentiality of voting information and the privacy of authority. Finally, the security of the new e-voting system is analyzed which includes eligibility, privacy, universal verifiability, uncoercibility, unreusability and fairness.
Oscillation : bioadéquation et bien-être sont-ils l'avenir d'un progrès durable pour nos enfants ?
Authors: Raffegeau Franck , Tromeur Eric
Published: October 2015

Dans les décennies à venir, l'adaptation au changement climatique est une question vitale pour la perpétuation des espèces végétales et animales, dont l’espèce humaine. Alors vers quel type d'adaptation souhaitons-nous aller ? L’usage du mot « adaptation » est aujourd'hui confus. Les notions « d’adaptation », de dynamiques de développement des espèces, dont celle de l’humain et de leurs « relations de dépendances » semblent mal comprises par les leaders d’opinion, les décideurs publics et les profanes. La question de l’adaptation au réchauffement climatique est-elle bien posée en termes de bien-être d’humanité et de progrès sociétal ? Les auteurs s’interrogent sur la hauteur de vue que l'humain doit atteindre pour une meilleure vision de son devenir dans et sur la nature, mais aussi de son épanouissement relationnel et comportemental avec les autres espèces pour un mieux-être comme source de bien-être et indicateur de progrès. Trois perspectives différentes de la conception commune de l'adaptation dans la société occidentale sont proposées : la bioadéquation, la compensation et l'adaptation. Ces trois sens contribuent à replacer l'humain comme être biologique et espèce animale vivant en relation avec d’autres espèces domestiques et sauvages. Aussi dans le contexte de notre dynamique de développement en zones urbaines et périurbaines, ce discernement participe à ouvrir le débat sur les aptitudes réelles de réponses des espèces face aux aléas du changement climatique.
Using Statistical Model of Failure Mode Effect Analysis for Risk Factors in Industrial Logistics
Authors: Prusa Petr , Kuptcova Anastasia , Chocholac Jan , Krile Srecko , Nadvornik Miroslav
Published: October 2015

The paper presents Six-Sigma methodologies, which is powerful method for increasing quality and development of manufacturing, by understanding risk factors, can suggest proper and thoughtful decision-making that may protect the business plan from causes. This study examines a framework of improved performance under the possible influence of different failure modes. Econometric verification presents statistical assumptions for the application of estimated procedures in the area of logistics, especially in the area of industrial logistics. The statistical method for the methodology is to test statistical reliability and the significance of estimated parameters for the analysis of numerical values of risk priority.
Possible Use of Industrial Robots for Handling Operations in Transport and Logistics
Authors: Lizbetin Jan , Caha Zdenek , Vejs Petr
Published: October 2015

Current trends in the industrial or any other economic sector is to mechanize and gradually to automate individual procedures through the use of various technologies. The purpose of this is to minimize strenuous human labour and eliminate human error. Robotic automation can be effectively used in material handling operations, whether it is a logistics warehouse operation or a transport loading operation. This article deals with the characteristics of different types of industrial robots. It compares the different types of robots in terms of their appropriate application and consequently defines the requirements for those robots that can be applied in handling operations in transport and logistics.
Public Transport Preference as the Tool to Manage Traffic Flow and to Increase Service Quality from a Customer Point of View
Authors: Kostolna Maria , Kubikova Simona , Konecny Vladimir
Published: October 2015

The increase of individual car traffic causes that existing transport networks fail to meet the needs of road traffic in the means of capacity. One of the possible options for solving this problem is the construction of new communications. This solution is difficult to implement due to the high investment costs and possibility for further development. Therefore, one of the options for avoiding this problem is to make public urban transport more attractive, for example, through its support and preference. One of the main criteria in the quality evaluation of public urban transport is the transfer speed from origin to destination. This speed can be most noticeably affected by the use of public urban transport preference.This paper will discuss the analysis of passengers’ satisfaction with services provided by carriers of urban transport and the possibilities of public urban transport preference through the preference of public urban transport vehicles in reserved traffic lanes and on signal controlled intersections.
Development of Mechatronic Deformation System
Authors: Martikan Milan , Brumercik Frantisek , Bastovansky Ronald
Published: October 2015

The paper describes a comprehensive management system concept and implementation of the development of the production strain crystallization vessel of molybdenum sheet for the production of single crystal sapphire.
Design Modification of Test Device for Analyzing of Conveyor Belt Samples
Authors: Molnar Vieroslav , Michalik Peter
Published: October 2015

Material testing and its analysis is nowadays a very extensive problem. Different types of materials with different sizes are tested and analyzed. By the Technical University of Košice was designed the test device for analysis of cover layers samples for conveyor belts designed for bulk materials conveying for middle and long distances in various direction with the diameters from 100 mm to 300 mm. Measurement of samples helps for the better understanding and determination of cover layers properties and it allows by measured values the next computing simulations. This paper deals with constructive modifications of the designed test device which are described in detail in this paper. For creation of the model of the test device was used SW application PTC Creo 2.0.
Suggestion for Modification of Qualitative Requirements for Bitumen Sheets within Manufacturing Industry
Authors: Plachý Jan , Vysoka Jana , Vejmelka Radek , Vanickova Radka
Published: October 2015

The aim of this report is to evolve the suggestion for determining the limiting value of the qualitative requirements for the flow resistance during high temperatures for reinforced bitumen sheets that are used as the waterproofing of the concrete bridge decks due to EN 14695:2010 and ČSN 736242:2010. Suggested revisions of the standards will lead manufacturers to improve the bitumen sheets which are affecting the processability when using in practice.
Efficiency of Retrieval Process
Authors: Cujan Zdenek , Fedorko Gabriel
Published: October 2015

The paper deals with the process of assembling which is one of the most important process of store management. Activities related to the assembling and the way of this process organization and realization has significantly impact not only on the accuracy and completeness of consignments but also on the quality of created logistics units. The way of realization of the assembling process is closely associated with its effectivity and with total costs.
Fabrication of Polycaprolactone/Centella asiatica Extract Biopolymer Nanofiber by Electrospinning
Authors: Manotham Supalak , Pengpat Kamonpan , Eitssayeam Sukum , Rujijanagul Gobwute , Sweatman Denis Russell , Tunkasiri Tawee
Published: October 2015

This work aims to fabricate and investigate biopolymer electrospun nanofibers of polycaprolactone (PCL) mixed with Centella asiatica (C. asiatica). The nanofibers were manufactured by electrospinning technique. C. asiatica were extracted and loaded into the nanofibers with different contents (0.5, 2.5, 5 and 10 wt%). Fourier transform infrared analysis indicated that both PCL and C. Asiatica presented in the nanofibers without chemical reaction between them. The morphology of the nanofibers was investigated by a scanning electron microscope, exhibiting smooth and bead-free with diameters of 300 to 500 nm. Mechanical properties of the fibers show that the tensile stress notable increased with the C. Asiatica content. Antimicrobial activity test showed that this material had a potential for anti-bactericidal activity.
Antioxidant Activity in the Petal Extract of PAP1 Transgenic Tobacco
Authors: Kanthang Supha , Sompornpailin Kanokporn
Published: October 2015

Flavonoids are compounds which act as antioxidants in both plant and human. These substances are found in reproductive tissues. Tobaccos expressing ORF of PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) involved in flavonoid biosynthesis were used for investigating the flavonoid profiles and antioxidant activity of petals and compared to wild type (WT) tobacco. Approximately two months, the tobacco grown in a culturing room had produced flowers for harvesting. The petal tissues were extracted with the solvent of acidic methanol and water. These extracts were measured the specific wavelength of flavonoid derivatives by using a spectrophotometer. The petal extract of H7 line showed the highest content of all detected flavonoid subgroups. However the petal extract of transgenic line H5 and H7 had significantly higher levels of anthocyanin (pelargonidin) than those of the other transgenic and WT tobaccos. An antioxidant activity of the petal extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) assay. An effective concentration of the extract which scavenged DPPH radical by 50% (EC50) was presented. EC50 of the H5 and H7 transgenic extracts was approximately two folds less than that of WT extract Therefore anthocyanin may highly affect on antioxidant activities.
Colour Fastness Property of Printed Cotton Fabric Using Warp Yarn Printing Technique
Authors: Ariyakuare Kittisak , Klaichoi Charoon , Phaisarntantiwong Naruepon , Senatham Songkram
Published: October 2015

Cotton fabric samples were printed with pigment dye using warp yarn printing technique has been investigated. The color fastness was evaluated to testing in with washing, light, rubbing, water, and perspiration of the printed samples, the test were determined according to ISO 105-C06 A1S: 2010, ISO 105-B02: 1994, ISO 105-X12: 2001, ISO 105-E01: 2010, and ISO 105-E04: 2008 respectively. The results showed that the colour fastness to washing, water, and perspiration were in very good level whereas colour fastness to rubbing was in good. The results indicated that the properties of printed samples (handling and sharpness) were good to very good level.
Practical Homemade Copper Filter by Banana Peels, Sand and Charcoal for Wastewater Treatment
Authors: Nicomrat Duongruitai , Tharajak Jirasak
Published: October 2015

Copper ions are commonly contaminated in the effluents from many electronic factory. In copper filtration, most filter types are usually expensive and causes toxic residues and creates another health and environmental problem. Therefore, this research has been developed a copper filtration approach based on homemade non-hazardous residues of banana peels, unmeshed sand, and charcoal. The results showed that baked, minced banana peels media could absorb synthetic water having copper ion at 50 ppm with filtration efficiency of 70% within 2 hr. Baked minced banana peels in combination with sand and charcoal could adsorb Copper (II) at 50 mg/ mL more than 80%. However, the swollen structure of banana peels during copper (II) filtration caused limit filtration efficiency to 4-5 hour extraction period. The tentative wastewater treatment application is, therefore, the use of bio-adsorbent for effective adsorption of toxic heavy metals from effluents open discarded from agricultural wastes in the environment.