bluuebunny/crossref_metadata_2025 huggingface dataset:
Speech Enhancement for Hearing Aids with Deep Learning on Environmental Noises
Authors: Park Gyuseok , Cho Woohyeong , Kim Kyu-Sung , Lee Sangmin
Published: September 2020

Hearing aids are small electronic devices designed to improve hearing for persons with impaired hearing, using sophisticated audio signal processing algorithms and technologies. In general, the speech enhancement algorithms in hearing aids remove the environmental noise and enhance speech while still giving consideration to hearing characteristics and the environmental surroundings. In this study, a speech enhancement algorithm was proposed to improve speech quality in a hearing aid environment by applying noise reduction algorithms with deep neural network learning based on noise classification. In order to evaluate the speech enhancement in an actual hearing aid environment, ten types of noise were self-recorded and classified using convolutional neural networks. In addition, noise reduction for speech enhancement in the hearing aid were applied by deep neural networks based on the noise classification. As a result, the speech quality based on the speech enhancements removed using the deep neural networks—and associated environmental noise classification—exhibited a significant improvement over that of the conventional hearing aid algorithm. The improved speech quality was also evaluated by objective measure through the perceptual evaluation of speech quality score, the short-time objective intelligibility score, the overall quality composite measure, and the log likelihood ratio score.
Improvement of Quantum Dot Light Emitting Device Characteristics by CdSe/ZnS Blended with HMDS (Hexamethyldisilazane)
Authors: Park Junekyun , Shin Eunkyu , Park Jongwoo , Roh Yonghan
Published: September 2020

We demonstrated the way to improve the characteristics of quantum dot light emitting diodes (QD-LEDs) by adding a simple step to the conventional fabrication process. For instance, we can effectively deactivate the surface defects of quantum dot (QD) (e.g., CdSe/ZnS core-shell QDs in the current work) with the SiO bonds by simply mixing QDs with hexamethyldisilazane (HMDS) under atmospheric conditions. We observed the substantial improvement of device characteristics such that the current efficiency, the maximum luminance, and the QD lifetime were improved by 1.7–1.8 times, 15–18%, and nine times, respectively, by employing this process. Based on the experimental data (e.g., energy dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS)), we estimated that the growth of the SiOx on the surface of QDs is self-limited: the SiOx are effective to passivate the surface defects of QDs without deteriorating the intrinsic properties including the color-purity of QDs. Second, we proposed that the emission profiling study can lead us to the fundamental understanding of charge flow in each layer of QD-LEDs. Interestingly enough, many problems related to the charge-imbalance phenomenon were simply solved by selecting the combination of thicknesses of the hole transport layer (HTL) and the electron transport layer (ETL).
Hybrid Engineered Water–Polymer Flooding in Carbonates: A Review of Mechanisms and Case Studies
Authors: Shakeel Mariam , Pourafshary Peyman , Rehan Hashmet Muhammad
Published: September 2020

The fast depletion of oil reserves has steered the petroleum industry towards developing novel and cost-effective enhanced oil recovery (EOR) techniques in order to get the most out of reservoirs. Engineered water–polymer flooding (EWPF) is an emerging hybrid EOR technology that uses the synergetic effects of engineered water (EW) and polymers to enhance both the microscopic and macroscopic sweep efficiencies, which mainly results from: (1) the low-salinity effect and the presence of active ions in EW, which help in detachment of carboxylic oil material from the rock surface, wettability alteration, and reduction in the residual oil saturation; (2) the favorable mobility ratio resulting from the use of a polymer; and (3) the improved thermal and salinity resistance of polymers in EW. Various underlying mechanisms have been proposed in the literature for EW EOR effects in carbonates, but the main driving factors still need to be understood properly. Both polymer flooding (PF) and EW have associated merits and demerits. However, the demerits of each can be overcome by combining the two methods, known as hybrid EWPF. This hybrid technique has been experimentally investigated for both sandstone and carbonate reservoirs by various researchers. Most of the studies have shown the synergistic benefits of the hybrid method in terms of two- to four-fold decreases in the polymer adsorption, leading to 30–50% reductions in polymer consumption, making the project economically viable for carbonates. EWPF has resulted in 20–30% extra oil recovery in various carbonate coreflood experiments compared to high-salinity water flooding. This review presents insights into the use of hybrid EWPF for carbonates, the main recovery driving factors in the hybrid process, the advantages and limitations of this method, and some areas requiring further work.
Vibration Reduction Strategy for Offshore Wind Turbines
Authors: Liu Haoming , Yang Suxiang , Tian Wei , Zhao Min , Yuan Xiaoling , Xu Bofeng
Published: September 2020

The operational environment of offshore wind turbines is much more complex than that of onshore wind turbines. Facing the persistent wind and wave forces, offshore wind turbines are prone to vibration problems, which are not conducive to their long-term operation. Under this background, first, how the wave affects the vibration characteristics of offshore wind turbines is analyzed. Based on the existing wave and wave load models, we analytically show that there exist fluctuating components related to the hydrodynamic frequency in the aerodynamic load and aerodynamic torque of offshore wind turbines. Simulation results based on a GH Bladed platform further validates the analysis. Second, in order to reduce the joint impacts of the wave, wind shear and tower shadow on the wind turbine, a variable pitch control method is proposed. The integrated tower top vibration acceleration signal is superimposed on the collective pitch reference signal, then the triple frequency (3P) fluctuating component of the wind turbine output power and the azimuth angle of each blade are converted into the pitch angle adjustment signal of each blade, which is superimposed on the collective pitch signal for individual pitch control. The simulation results show that the proposed pitch control strategy can effectively smooth the fluctuation of blade root flap-wise load caused by wind and wave, and significantly reduce the fluctuation of aerodynamic torque and output power of offshore wind turbines.
Proposal of a Methodological Universal Guide to (Re)discover the Heritage: Palazzo Gastaldi-Lavagna
Authors: Besana Daniela , Chiesa Andrea
Published: September 2020

This paper analyzes the theme of the knowledge project as an element guide of the restoration project. In view of the constant evolution of technologies related to diagnostics (in the laboratory and in situ), the research exposes the importance of a comprehensive acquisition of preliminary information as a project design phase tool, in order to solve doubts, verify hypotheses preventing the timeline and control economical aspects during the construction phase. The case study analyzes the restoration of an eighteenth-century apartment in a noble palace of Imperia City that has been neglected for years. From a first phase of analysis and knowledge, the project action sought to integrate contemporary needs with the historical features, in respect of the existing and its conservation. The proposed intervention aims to show how the survey of the building (geometric, material and decorative) combined with the investigations on the existing unit (archival, historical-architectural, technical-constructive and geological-petrographic) and the diagnosis of the pathological picture are necessary knowledge to guide and control actions of the final phase of the project.
Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on Enterococcus faecalis Biofilm
Authors: Wigler Ronald , Matalon Shlomo , Goldberger Tomer , Lerner Anat Or , Kfir Anda
Published: September 2020

This study aimed to determine the bactericidal efficacy of sequential use of NaOCl pH 12 followed by acidified NaOCl pH 6.5, and compare it to that of either of these NaOCl solutions alone. E. faecalis biofilm was grown on standardized dentine specimens for four weeks. The specimens were randomly divided into four groups: (A) 4 min exposure to 0.9% saline solution (control); (B) 4 min exposure to 4% NaOCl pH 12; (C) 4 min exposure to 4% NaOCl pH 6.5; and (D) 2 min exposure to 4% NaOCl pH 12 followed by 2 min exposure to 4% NaOCl pH 6.5. The bactericidal activity was evaluated after the 4 min of contact time using confocal laser scanning microscopy. The volume ratio of red fluorescence to green and red fluorescence indicated the proportion of dead cells in the biofilm. The percent of dead cells in the saline solution group was significantly lower than those in the other groups. There was no significant difference between NaOCl pH 12 compared to NaOCl pH 6.5. The sequential use of NaOCl pH 12 followed by pH 6.5 significantly increased the percent of dead cells compared to both the samples exposed to either NaOCl pH 12 or pH 6.5. These results show that sequential irrigation protocol had a stronger bactericidal effect than the commonly used NaOCl pH 12.
Impacts of Killing Process on the Nutrient Content, Product Stability and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae Meals
Authors: Zhen Yongkang , Chundang Pipatpong , Zhang Yu , Wang Mengzhi , Vongsangnak Wanwipa , Pruksakorn Chantima , Kovitvadhi Attawit
Published: September 2020

The black soldier fly (BSF, Hermetia illucens) is considered a potential sustainable insect alternative source of protein for animal feed. The quality of a BSF meal is greatly influenced by the killing method and the purpose of this article is to compare the influences of different killing methods. BSFs at the 18-day-old prepupae stage were separated into six different killing methods with three replicates: 1. blending, 2. freezing, 3. CO2 treatment, 4. vacuum, 5. blanching and 6. CO2 plus blanching. After killing, BSF larvae meals were obtained by hot air oven drying and grinding. The chemical composition and in vitro digestibility calculated from sediments were not affected by the killing method, except that blending provided the worst BSF quality for all measured parameters (p < 0.05). The highest quality of BSF was obtained from the heat treatment procedures (blanching and the CO2 plus blanching methods), as they produced lower acidity after killing, total viable counts, browning reaction (enzymatic and non-enzymatic), darkness, moisture, fat acidity, protein and lipid oxidation during storage compared with other killing procedures (p < 0.05). Interestingly, the highest free amino acids in the supernatant after in vitro digestibility of BSF samples was observed with the CO2 plus blanching killing method (p < 0.05), whereas other parameters were similar to those obtained with blanching. The CO2 plus blanching method did not produce clearly different outcomes to blanching; therefore, the selection of one of these techniques over the other should depend on the regulations in each country.
Termite Resistance of Furfuryl Alcohol and Imidacloprid Treated Fast-Growing Tropical Wood Species as Function of Field Test
Authors: Hadi Yusuf Sudo , Herliyana Elis Nina , Mulyosari Desy , Abdillah Imam Busyra , Pari Rohmah , Hiziroglu Salim
Published: September 2020

In general fast-growing tree species harvested at a young age has substantial amount of sapwood. It also contains juvenile wood, which has undesirable inferior physical and mechanical properties. Having sapwood and juvenile wood in the trees makes them very susceptible to be attacked by biological deterioration specifically termites in a tropical environment. The main objective of this study was to investigate the termite resistance of four fast-growing Indonesian wood species treated with furfuryl alcohol and imidacloprid. Wood specimens from sengon (Falcataria moluccana), jabon (Anthocephalus cadamba), mangium (Acacia mangium), and pine (Pinus merkusii) were impregnated with furfuryl alcohol, using tartaric acid and heat as well as treated with imidacloprid for the polymerization process. All of the specimens were exposed to environmental conditions in the field for three months. Based on the findings in this work, the untreated control samples had higher weight loss values and lower protection levels than those of imidacloprid-treated and furfurylated samples of all four species. It appears that furfurylation and imidacloprid treatment of such fast-growing species had a significant impact regarding their resistance against termite so that their service life can be extended during their utilization.
Application of Unmanned Aerial Vehicle (UAV)-Acquired Topography for Quantifying Typhoon-Driven Landslide Volume and Its Potential Topographic Impact on Rivers in Mountainous Catchments
Authors: Chang Kuo-Jen , Tseng Chun-Wei , Tseng Chih-Ming , Liao Ta-Chun , Yang Ci-Jian
Published: September 2020

Landslides are highly erosional processes that dominate sediment mobilization and reshape landscapes in orogenic belts. Therefore, quantifying and characterizing landslide volume is essential to disaster prevention and understanding landscape evolution in mountainous rivers. Progressive development of the structure-from-motion (SfM) and multi-view stereo (MVS) photogrammetric techniques and Unmanned Aerial Vehicles (UAV) provides low-cost and high-resolution digital elevation models (DEMs), compared to traditional aerial photogrammetry at the same resolution. In this study, we quantified landslide volume and change in river channel volume at meter-scale accuracy for the Laishe River catchment of southern Taiwan from 2009 to 2015, which provides reliable data for discussing sediment transport and morphological response. The observations indicate that Typhoon Morakot in August 2009, induced a landslide volume of 31.63 million (M) m3, which is equal to 87% of the six-year sediment production. Typhoon Morakot also caused the deposition of 8.2 M m3 in the Laishe River. Additionally, this study demonstrates the feasibility of using UAVs to quantify the migration of landslide material and changes in channel area and volume, and the detection of landslide dams. In conclusion, two sources of images, especially those by UAVs, were used to decipher the consequence and potential hazard, social impact, and morphological changes in a mountainous river.
Optical Power Budget of 25+ Gbps IM/DD PON with Digital Signal Post-Equalization
Authors: Udalcovs Aleksejs , Salgals Toms , Zhang Lu , Pang Xiaodan , Djupsjöbacka Anders , Spolitis Sandis , Bobrovs Vjaceslavs , Popov Sergei , Ozolins Oskars
Published: September 2020

While infrastructure providers are expanding their portfolio to offer sustainable solutions for beyond 10 Gbps in the access segment of optical networks, we experimentally compare several modulation format alternatives for future passive optical networks (PONs) aiming to deliver 25+ Gbps net-rates. As promising candidates, we consider the intensity modulation direct detection (IM/DD) schemes such as electrical duobinary (EDB) and 4-level and 8-level pulse amplitude modulations (PAM-4/8). They are more spectrally efficient than the conventional non-return-to-zero on-off-keying (NRZ-OOK) used in current 10G PONs. As we move to higher rates, digital equalization enhances the performance by smoothening the systems imperfection. However, the impact that such equalization has on the optical power budget remains unclear. Therefore, in this article, we fairly compare the optical power budget values of a time division multiplexed PON (TDM-PON) exploiting a linear digital signal equalization at the receiver side. We consider the conventional PON configuration (20 km of single-mode fiber (SMF), 1:N optical power splitting) with IM/DD and net-rates above 25 Gbps. Furthermore, we focus on a downstream transmission imposing the bandwidth limitations of 10G components using a digital filter before the detection. The obtained results show that the use of a digital post-equalization with 43 feed-forward (FF) and 21 feedback (FB) taps can significantly improve the signal quality enabling new alternatives and enhancing the optical power budget.
An Experimentally Defined Hypoxia Gene Signature in Glioblastoma and Its Modulation by Metformin
Authors: Calvo Tardón Marta , Marinari Eliana , Migliorini Denis , Bes Viviane , Tankov Stoyan , Charrier Emily , McKee Thomas A , Dutoit Valérie , Dietrich Pierre-Yves , Cosset Erika , Walker Paul R
Published: September 2020

Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor, characterized by a high degree of intertumoral heterogeneity. However, a common feature of the GBM microenvironment is hypoxia, which can promote radio- and chemotherapy resistance, immunosuppression, angiogenesis, and stemness. We experimentally defined common GBM adaptations to physiologically relevant oxygen gradients, and we assessed their modulation by the metabolic drug metformin. We directly exposed human GBM cell lines to hypoxia (1% O2) and to physioxia (5% O2). We then performed transcriptional profiling and compared our in vitro findings to predicted hypoxic areas in vivo using in silico analyses. We observed a heterogenous hypoxia response, but also a common gene signature that was induced by a physiologically relevant change in oxygenation from 5% O2 to 1% O2. In silico analyses showed that this hypoxia signature was highly correlated with a perinecrotic localization in GBM tumors, expression of certain glycolytic and immune-related genes, and poor prognosis of GBM patients. Metformin treatment of GBM cell lines under hypoxia and physioxia reduced viable cell number, oxygen consumption rate, and partially reversed the hypoxia gene signature, supporting further exploration of targeting tumor metabolism as a treatment component for hypoxic GBM.
8-Deoxy-Rifamycin Derivatives from Amycolatopsis mediterranei S699 ΔrifT Strain
Authors: Ye Feng , Shi Yanrong , Zhao Shengliang , Li Zhiying , Wang Haoxin , Lu Chunhua , Shen Yuemao
Published: September 2020

Proansamycin X, a hypothetical earliest macrocyclic precursor in the biosynthesis of rifamycin, had never been isolated and identified. According to bioinformatics analysis, it was proposed that RifT (a putative NADH-dependent dehydrogenase) may be a candidate target responsible for the dehydrogenation of proansamycin X. In this study, the mutant strain Amycolatopsis mediterranei S699 ΔrifT was constructed by deleting the rifT gene. From this strain, eleven 8-deoxy-rifamycin derivatives (1–11) and seven known analogues (12–18) were isolated. Their structures were elucidated by extensive analysis of 1D and 2D NMR spectroscopic data and high-resolution ESI mass spectra. Compound 1 is a novel amide N-glycoside of seco-rifamycin. Compounds 2 and 3 feature conserved 11,12-seco-rifamycin W skeleton. The diverse post-modifications in the polyketide chain led to the production of 4–11. Compounds 2, 3, 5, 6, 13 and 15 exhibited antibacterial activity against Staphylococcus aureus (MIC (minimal inhibitory concentration) values of 10, 20, 20, 20, 40 and 20 μg/mL, respectively). Compounds 14, 15, 16, 17 and 18 showed potent antiproliferative activity against KG1 cells with IC50 (half maximal inhibitory concentration) values of 14.91, 44.78, 2.16, 18.67 and 8.07 μM, respectively.
Targeting MHC Regulation Using Polycyclic Polyprenylated Acylphloroglucinols Isolated from Garcinia bancana
Authors: Coste Chloé , Gérard Nathalie , Dinh Chau Phi , Bruguière Antoine , Rouger Caroline , Leong Sow Tein , Awang Khalijah , Richomme Pascal , Derbré Séverine , Charreau Béatrice
Published: September 2020

Modulation of major histocompatibility complex (MHC) expression using drugs has been proposed to control immunity. Phytochemical investigations on Garcinia species have allowed the isolation of bioactive compounds such as polycyclic polyprenylated acylphloroglucinols (PPAPs). PPAPs such as guttiferone J (1), display anti-inflammatory and immunoregulatory activities while garcinol (4) is a histone acetyltransferases (HAT) p300 inhibitor. This study reports on the isolation, identification and biological characterization of two other PPAPs, i.e., xanthochymol (2) and guttiferone F (3) from Garcinia bancana, sharing structural analogy with guttiferone J (1) and garcinol (4). We show that PPAPs 1–4 efficiently downregulated the expression of several MHC molecules (HLA-class I, -class II, MICA/B and HLA-E) at the surface of human primary endothelial cells upon inflammation. Mechanistically, PPAPs 1–4 reduce MHC proteins by decreasing the expression and phosphorylation of the transcription factor STAT1 involved in MHC upregulation mediated by IFN-γ. Loss of STAT1 activity results from inhibition of HAT CBP/p300 activity reflected by a hypoacetylation state. The binding interactions to p300 were confirmed through molecular docking. Loss of STAT1 impairs the expression of CIITA and GATA2 but also TAP1 and Tapasin required for peptide loading and transport of MHC. Overall, we identified new PPAPs issued from Garcinia bancana with potential immunoregulatory properties.
The Antimicrobial Cathelicidin CRAMP Augments Platelet Activation during Psoriasis in Mice
Authors: Salamah Maryam F. , Vallance Thomas M. , Kodji Xenia , Ravishankar Divyashree , Williams Harry F. , Brain Susan D. , Vaiyapuri Sakthivel
Published: September 2020

Platelet-associated complications including thrombosis, thrombocytopenia, and haemorrhage are commonly observed during various inflammatory diseases such as psoriasis. Although several mechanisms that may contribute to the dysfunction of platelets during inflammatory diseases have been reported, knowledge on the primary molecules/mechanisms that underpin platelet-associated complications in such conditions is not fully established. Here, we report the significance of the mouse antimicrobial cathelicidin, mouse cathelicidin-related antimicrobial peptide (mCRAMP) (an orthologue of LL37 in humans), on the modulation of platelet reactivity during psoriasis using Imiquimod-induced psoriasis in mice as an inflammatory disease model for psoriasis vulgaris in humans. The activation of platelets during psoriasis is increased as evidenced by the elevated levels of fibrinogen binding and P-selectin exposure on the surface of platelets, and the level of soluble P-selectin in the plasma of psoriatic mice. The skin and plasma of psoriatic mice displayed increased levels of mCRAMP. Moreover, the plasma of psoriatic mice augmented the activation of platelets obtained from healthy mice. The effect of mCRAMP is partially mediated through formyl peptide receptor 2/3 (Fpr2/3, the orthologue to human FPR2/ALX) in platelets as a significant reduction in their activation was observed when FPR2/ALX-selective inhibitors such as WRW4 or Fpr2/3-deficient mouse platelets were used in these assays. Since the level of antimicrobial cathelicidin is increased in numerous inflammatory diseases such as psoriasis, atherosclerosis, and inflammatory bowel disease, the results of this study point towards a critical role for antimicrobial cathelicidin and FPR2/ALX in the development of platelet-related complications in such diseases.
Single-Cell NGS-Based Analysis of Copy Number Alterations Reveals New Insights in Circulating Tumor Cells Persistence in Early-Stage Breast Cancer
Authors: Rossi Tania , Gallerani Giulia , Angeli Davide , Cocchi Claudia , Bandini Erika , Fici Pietro , Gaudio Michele , Martinelli Giovanni , Rocca Andrea , Maltoni Roberta , Fabbri Francesco
Published: September 2020

Circulating tumor cells (CTCs) are a rare population of cells representing a key player in the metastatic cascade. They are recognized as a validated tool for the identification of patients with a higher risk of relapse, including those diagnosed with breast cancer (BC). However, CTCs are characterized by high levels of heterogeneity that also involve copy number alterations (CNAs), structural variations associated with gene dosage changes. In this study, single CTCs were isolated from the peripheral blood of 11 early-stage BC patients at different time points. A label-free enrichment of CTCs was performed using OncoQuick, and single CTCs were isolated using DEPArray. Libraries were prepared from single CTCs and DNA extracted from matched tumor tissues for a whole-genome low-coverage next-generation sequencing (NGS) analysis using the Ion Torrent S5 System. The analysis of the CNA burden highlighted that CTCs had different degrees of aberration based on the time point and subtype. CTCs were found even six months after surgery and shared CNAs with matched tumor tissue. Tumor-associated CNAs that were recurrent in CTCs were patient-specific, and some alterations involved regions associated with BC and survival (i.e., gains at 1q21-23 and 5p15.33). The enrichment analysis emphasized the involvement of aberrations of terms, associated in particular with interferon (IFN) signaling. Collectively, our findings reveal that these aberrations may contribute to understanding the molecular mechanisms involving CTC-related processes and their survival ability in occult niches, supporting the goal of exploiting their application in patients’ surveillance and follow-up.
Valorization of Cellulose Recovered from WWTP Sludge to Added Value Levulinic Acid with a Brønsted Acidic Ionic Liquid
Authors: Glińska Katarzyna , Lerigoleur Clara , Giralt Jaume , Torrens Esther , Bengoa Christophe
Published: September 2020

The progressive decline of using fossil sources in the industry means that alternative resources must be found to produce chemicals. Waste biomass (sewage sludge) and waste lignocellulosic resources (food, forestry, or paper industries) are ideal candidates to take over from fossil sources. Municipal sewage sludge, and especially primary sludge, has a significant proportion of cellulose in its composition. Proper treatment of this cellulose allows the production of interesting chemicals like levulinic acid that are precursors (bio-blocks or building blocks) for other organic chemical processes. Cellulose was extracted from municipal wet primary sludge and paper industry dried sludge with a commercial ionic liquid. More than 99% of the cellulose has been recovered in both cases. Extraction was followed by the bleaching of the cellulose for its purification. In the bleaching, a large part of the ash was removed (up to 70% with municipal sludge). Finally, the purified cellulose was converted in levulinic acid by catalyzed hydrothermal liquefaction. The reaction, done at 170 °C and 7 bar, catalyzed by a tailored Brønsted acidic ionic liquid produced levulinic acid and other by-products in smaller quantities. The process had a conversion of cellulose to levulinic acid of 0.25 with municipal sludge and of 0.31 with industrial sludge. These results fully justify the process but, require further study to increase the conversion of cellulose to levulinic acid.
A Translational Mouse Model for NASH with Advanced Fibrosis and Atherosclerosis Expressing Key Pathways of Human Pathology
Authors: van den Hoek Anita M. , Verschuren Lars , Worms Nicole , van Nieuwkoop Anita , de Ruiter Christa , Attema Joline , Menke Aswin L. , Caspers Martien P. M. , Radhakrishnan Sridhar , Salic Kanita , Kleemann Robert
Published: September 2020

Non-alcoholic steatohepatitis (NASH) is a fast-growing liver disorder that is associated with an increased incidence of cardiovascular disease and type 2 diabetes. Animal models adequately mimicking this condition are scarce. We herein investigate whether Ldlr−/−. Leiden mice on different high-fat diets represent a suitable NASH model. Ldlr−/−. Leiden mice were fed a healthy chow diet or fed a high-fat diet (HFD) containing lard or a fast food diet (FFD) containing milk fat. Additionally, the response to treatment with obeticholic acid (OCA) was evaluated. Both high-fat diets induced obesity, hyperlipidemia, hyperinsulinemia, and increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Mice on both diets developed progressive macro- and microvesicular steatosis, hepatic inflammation, and fibrosis, along with atherosclerosis. HFD induced more severe hyperinsulinemia, while FFD induced more severe hepatic inflammation with advanced (F3) bridging fibrosis, as well as more severe atherosclerosis. OCA treatment significantly reduced hepatic inflammation and fibrosis, and it did not affect atherosclerosis. Hepatic transcriptome analysis was compared with human NASH and illustrated similarity. The present study defines a translational model of NASH with progressive liver fibrosis and simultaneous atherosclerosis development. By adaptation of the fat content of the diet, either insulin resistance (HFD) or hepatic inflammation and fibrosis (FFD) can be aggravated.
Novel Opportunities for Cathepsin S Inhibitors in Cancer Immunotherapy by Nanocarrier-Mediated Delivery
Authors: Fuchs Natalie , Meta Mergim , Schuppan Detlef , Nuhn Lutz , Schirmeister Tanja
Published: September 2020

Cathepsin S (CatS) is a secreted cysteine protease that cleaves certain extracellular matrix proteins, regulates antigen presentation in antigen-presenting cells (APC), and promotes M2-type macrophage and dendritic cell polarization. CatS is overexpressed in many solid cancers, and overall, it appears to promote an immune-suppressive and tumor-promoting microenvironment. While most data suggest that CatS inhibition or knockdown promotes anti-cancer immunity, cell-specific inhibition, especially in myeloid cells, appears to be important for therapeutic efficacy. This makes the design of CatS selective inhibitors and their targeting to tumor-associated M2-type macrophages (TAM) and DC an attractive therapeutic strategy compared to the use of non-selective immunosuppressive compounds or untargeted approaches. The selective inhibition of CatS can be achieved through optimized small molecule inhibitors that show good pharmacokinetic profiles and are orally bioavailable. The targeting of these inhibitors to TAM is now more feasible using nanocarriers that are functionalized for a directed delivery. This review discusses the role of CatS in the immunological tumor microenvironment and upcoming possibilities for a nanocarrier-mediated delivery of potent and selective CatS inhibitors to TAM and related APC to promote anti-tumor immunity.
Effect of Annealing Process on Microstructure, Texture, and Mechanical Properties of a Fe-Si-Cr-Mo-C Deep Drawing Dual-Phase Steel
Authors: Pan Hongbo , Shen Xiaohui , Li Dongyang , Liu Yonggang , Cao Jinghua , Tian Yaqiang , Zhan Hua , Wang Huiting , Wang Zhigang , Xiao Yangyang
Published: September 2020

Dual phase steel generally has poor deep drawing property with a low r value less than 1.0, making it difficult to be used for deep drawing automotive parts. In order to improve the mechanical properties of the steel through heat treatment, effect of heat treatments with different conditions on a Fe-Si-Cr-Mo-C deep drawing dual-phase steel was investigated with the aim of identifying effective heat treatment parameters for effective modification towards optimal properties. Relevant thermal dilation and heat treatment experiments were performed. Corresponding characters were investigated. The results show that island martensite can be obtained at low cooling rate. With the increase of cooling rate, the formation of pearlite and bainite is favored. During annealing at low temperatures, recrystallization of the steel is incomplete with the presence of the shear bands. With the increase of annealing temperature, the recrystallization process is gradually complete, and the number of high angle grain boundaries increases significantly. The ratio of gamma orientation components to alpha orientation components decreases first and then increases with the increase of annealing temperature. The strain hardening exponent and r value show an upward trend with respect to annealing temperature, and the r value is as high as 1.15.
Parenting Styles and Academic Self-Efficacy Beliefs of Omani School and University Students
Authors: Aldhafri Said S. , Alrajhi Marwa N. , Alkharusi Hussain A. , Al-Harthy Ibrahim S. , Al-Barashdi Hafidha S. , Alhadabi Amal S.
Published: September 2020

The present study examined the predictive role of students’ perceptions of parenting styles on their academic efficacy beliefs. This relationship was examined using two large sets of national data that were collected from school and university students to see how the relationship between parenting styles and academic efficacy beliefs may or may not vary across life stages. The sample included 1431 school students and 1119 university students cross the Sultanate of Oman. The participants responded to the Arabic version of the Parental Authority Questionnaire (PAQ) and to the Academic Self-Efficacy Scale (ASES) constructed by the researchers. Using linear regression model for each sample, the results showed that the amount of variance in school students’ academic self-efficacy beliefs explained by parenting styles (R2 adjusted = 0.21) was higher than the amount of variance explained for the university sample (R2 adjusted = 0.10). The researchers concluded that the effects of parenting styles on students’ self-efficacy beliefs decrease as children grow up.
Estimation of the State of Charge of Lithium Batteries Based on Adaptive Unscented Kalman Filter Algorithm
Authors: Lv Jiechao , Jiang Baochen , Wang Xiaoli , Liu Yirong , Fu Yucheng
Published: September 2020

The state of charge (SOC) estimation of the battery is one of the important functions of the battery management system of the electric vehicle, and the accurate SOC estimation is of great significance to the safe operation of the electric vehicle and the service life of the battery. Among the existing SOC estimation methods, the unscented Kalman filter (UKF) algorithm is widely used for SOC estimation due to its lossless transformation and high estimation accuracy. However, the traditional UKF algorithm is greatly affected by system noise and observation noise during SOC estimation. Therefore, we took the lithium cobalt oxide battery as the analysis object, and designed an adaptive unscented Kalman filter (AUKF) algorithm based on innovation and residuals to estimate SOC. Firstly, the second-order RC equivalent circuit model was established according to the physical characteristics of the battery, and the least square method was used to identify the parameters of the model and verify the model accuracy. Then, the AUKF algorithm was used for SOC estimation; the AUKF algorithm monitors the changes of innovation and residual in the filter and updates system noise covariance and observation noise covariance in real time using innovation and residual, so as to adjust the gain of the filter and realize the optimal estimation. Finally came the error comparison analysis of the estimation results of the UKF algorithm and AUKF algorithm; the results prove that the accuracy of the AUKF algorithm is 2.6% better than that of UKF algorithm.
Model-Free Hybrid Control with Intelligent Proportional Integral and Super-Twisting Sliding Mode Control of PMSM Drives
Authors: Gao Peng , Zhang Guangming , Lv Xiaodong
Published: September 2020

In this research, based on the ultra-local model, a novel compound model-free control strategy with an intelligent Proportional-Integral and super-twisting Sliding Mode Control (MFiPISTSMC) strategy for permanent magnet synchronous motor (PMSM) drives is proposed. Firstly, an intelligent Proportional-Integral (iPI) control strategy is designed for motor speed regulation. Secondly, a super-twisting Sliding Mode Control (STSMC) strategy is constructed based on the ultra-local model of PMSM. At the same time, the unknown term of the ultra-local model of PMSM is estimated by a Linear Extended State Observer (LESO). The stability of the compound MFiPISTSMC strategy is proved by the Lyapunov stability theorem. As a result of the compound MFiPISTSMC strategy integrating the STSMC strategy, the iPI control strategy and the LESO is proposed to have excellent performance. Finally, the static characteristic, dynamic characteristic and robustness of the novel compound MFiPISTSMC strategy are verified by simulation and experimental results.
Backhaul-Aware Dimensioning and Planning of Millimeter-Wave Small Cell Networks
Authors: Muñoz Pablo , Adamuz-Hinojosa Oscar , Ameigeiras Pablo , Navarro-Ortiz Jorge , Ramos-Muñoz Juan J.
Published: September 2020

The massive deployment of Small Cells (SCs) is increasingly being adopted by mobile operators to face the exponentially growing traffic demand. Using the millimeter-wave (mmWave) band in the access and backhaul networks will be key to provide the capacity that meets such demand. However, dimensioning and planning have become complex tasks, because the capacity requirements for mmWave links can significantly vary with the SC location. In this work, we address the problem of SC planning considering the backhaul constraints, assuming that a line-of-sight (LOS) between the nodes is required to reliably support the traffic demand. Such a LOS condition reduces the set of potential site locations. Simulation results show that, under certain conditions, the proposed algorithm is effective in finding solutions and strongly efficient in computational cost when compared to exhaustive search approaches.
The Use of a Nutrient Quality Score is Effective to Assess the Overall Nutritional Value of Three Brassica Microgreens
Authors: Renna Massimiliano , Stellacci Anna Maria , Corbo Filomena , Santamaria Pietro
Published: September 2020

Microgreens have immense potential for improving dietary patterns, but little information is available regarding their overall nutritional value. We evaluated the nutritional traits of three hydroponically grown Brassica microgreens by using a Nutrient Quality Score. Micro cauliflower, micro broccoli and micro broccoli raab were grown using nutrient solutions with three different NH4:NO3 molar ratios (5:95, 15:85, and 25:75). Protein, dietary fiber, β-carotene, α-tocopherol and mineral elements (Ca, K, Mg, Fe, Zn, Cu, Mn, and Na) were analyzed. We developed the Nutrient Quality Score (NQS 11.1) on the basis of 11 desirable nutrients and 1 nutrient (sodium) to be limited. All Brassica microgreens are an excellent source of Vitamins A and E (more than 20% of the daily reference value—DRV), as well as a good source of calcium and manganese (10–19% of the DRV). Micro cauliflower showed a NQS 11.1 at 47% higher than micro broccoli raab and micro broccoli. Using NH4:NO3 25:75 molar ratio, the average score was 27% higher than other molar ratios. In all cases, the microgreens in the present study showed a higher NQS 11.1 than their mature counterpart (on the basis of data from the United States Department of Agriculture), highlighting that the score of micro cauliflower was about six-fold higher than mature cauliflower. In conclusion, the NQS 11.1 was useful for assessing the overall nutritional quality of the three Brassica microgreens, instead of simply quantifying nutrient content, in order to compare a single nutrient among different genotypes. Furthermore, the results highlight that the micro broccoli raab, micro broccoli and micro cauliflower in this study can be considered nutrient-rich vegetables that are able to improve dietary patterns more effectively than their mature counterparts.
An Overview of Population Projections—Methodological Concepts, International Data Availability, and Use Cases
Authors: Vanella Patrizio , Deschermeier Philipp , Wilke Christina B.
Published: September 2020

Population projections serve various actors at subnational, national, and international levels as a quantitative basis for political and economic decision-making. Usually, the users are no experts in statistics or forecasting and therefore lack the methodological and demographic background to completely understand methods and limitations behind the projections they use to inform further analysis. Our contribution primarily targets that readership. Therefore, we give a brief overview of different approaches to population projection and discuss their respective advantages and disadvantages, alongside practical problems in population data and forecasting. Fundamental differences between deterministic and stochastic approaches are discussed, with special emphasis on the advantages of stochastic approaches. Next to selected projection data available to the public, we show central areas of application of population projections, with an emphasis on Germany.
Biomarkers, Master Regulators and Genomic Fabric Remodeling in a Case of Papillary Thyroid Carcinoma
Authors: Iacobas Dumitru A.
Published: September 2020

Publicly available (own) transcriptomic data have been analyzed to quantify the alteration in functional pathways in thyroid cancer, establish the gene hierarchy, identify potential gene targets and predict the effects of their manipulation. The expression data have been generated by profiling one case of papillary thyroid carcinoma (PTC) and genetically manipulated BCPAP (papillary) and 8505C (anaplastic) human thyroid cancer cell lines. The study used the genomic fabric paradigm that considers the transcriptome as a multi-dimensional mathematical object based on the three independent characteristics that can be derived for each gene from the expression data. We found remarkable remodeling of the thyroid hormone synthesis, cell cycle, oxidative phosphorylation and apoptosis pathways. Serine peptidase inhibitor, Kunitz type, 2 (SPINT2) was identified as the Gene Master Regulator of the investigated PTC. The substantial increase in the expression synergism of SPINT2 with apoptosis genes in the cancer nodule with respect to the surrounding normal tissue (NOR) suggests that SPINT2 experimental overexpression may force the PTC cells into apoptosis with a negligible effect on the NOR cells. The predictive value of the expression coordination for the expression regulation was validated with data from 8505C and BCPAP cell lines before and after lentiviral transfection with DDX19B.
Occurrence of Pasteurella multocida in Dogs Being Trained for Animal-Assisted Therapy
Authors: Santaniello Antonio , Garzillo Susanne , Amato Alessia , Sansone Mario , Fioretti Alessandro , Menna Lucia Francesca
Published: September 2020

Animal-assisted therapy (AAT) is a non-pharmacological therapy aimed at people with physical and/or mental disabilities. Therefore, it is necessary to carry out interventions that guarantee its benefits for patients while also avoiding the risk of zoonoses due to contact with the animals or their mucous membranes. The present study aimed to detect the occurrence of Pasteurella multocida in the oral cavity of dogs attending a “dog educational centre” and training for AAT interventions. In addition, some of the potential predictable factors of infection (i.e., age, sex, breed, and living conditions) were analyzed. In total, 25/200 dogs examined (12.5%; 95% confidence interval = 8.4–18.1%) were positive for P. multocida, as confirmed by PCR. Sex, breed, and living conditions were risk factors associated with P. multocida as revealed by the logistic regression analysis. Specifically, cross-bred female dogs living prevalently outdoors were significantly associated with the presence of P. multocida (p < 0.05). This study represents the first epidemiological survey of the prevalence of P. multocida in the oral cavity of dogs involved subsequently in AAT interventions, highlighting the potential risk of P. multocida infection in patients, often belonging to risk categories (e.g., children, the elderly, and immunocompromised individuals). Therefore, healthcare guidelines could be suggested to integrate the current literature related to the health check of dogs involved in AAT. In this way, it could be ensured that, even with bodily contact during AAT, the risk of pathogen transmission by the co-therapist dog can be avoided.
Burden and Preparedness amongst Informal Caregivers of Adults with Moderate to Severe Traumatic Brain Injury
Authors: Lieshout Kirsten , Oates Joanne , Baker Anne , Unsworth Carolyn A. , Cameron Ian D. , Schmidt Julia , Lannin Natasha A.
Published: September 2020

This study examined the patterns of informal (unpaid) caregiving provided to people after moderate to severe traumatic brain injury (TBI), explore the self-reported burden and preparedness for the caregiving role, and identify factors predictive of caregiver burden and preparedness. A cross-sectional cohort design was used. Informal caregivers completed the Demand and Difficulty subscales of the Caregiving Burden Scale; and the Mutuality, Preparedness, and Global Strain subscales of the Family Care Inventory. Chi-square tests and logistic regression were used to examine the relationships between caregiver and care recipient variables and preparedness for caregiving. Twenty-nine informal caregivers who reported data on themselves and people with a moderate to severe TBI were recruited (referred to as a dyad). Most caregivers were female (n = 21, 72%), lived with the care recipient (n = 20, 69%), and reported high levels of burden on both scales. While most caregivers (n = 21, 72%) felt “pretty well” or “very well” prepared for caregiving, they were least prepared to get help or information from the health system, and to deal with the stress of caregiving. No significant relationships or predictors for caregiver burden or preparedness were identified. While caregivers reported the provision of care as both highly difficult and demanding, further research is required to better understand the reasons for the variability in caregiver experience, and ultimately how to best prepare caregivers for this long-term role.
Study Protocol for a Qualitative Research Project Exploring an Occupational Health Surveillance Model for Workers Exposed to Hand-Intensive Work
Authors: Eliasson Kristina , Palm Peter , Nordander Catarina , Dahlgren Gunilla , Lewis Charlotte , Hellman Therese , Svartengren Magnus , Nyman Teresia
Published: September 2020

The objective of this study protocol is to describe the development of a process model for occupational health surveillance for workers exposed to hand-intensive work (the HIW-model), and to describe the studies that will explore the model. The studies are designed to: (1) explore stakeholders’ experiences of the model, and (2) explore if, and how, the model affects actions for reduction of exposure to hand-intensive work. The study protocol presents a research project that is described as two studies. The first study will explore company representatives’ and ergonomists’ experiences of the execution of the HIW-model and its various components concerning feasibility and values. Semi-structured interviews will constitute the data source. The second study will explore whether the execution of the HIW-model leads to work environmental changes, such as actions for reduction of exposure to hand-intensive work, and whether these potential actions are based on the ergonomist’s feedback of the exposure assessment and the medical health checks. A mixed method approach will be applied, in which the data sources will be comprised of semi-structured interviews, questionnaires, and documents. The project is expected to generate knowledge regarding the values of the HIW-model. The project is anticipated to shed light on factors that facilitate or impede execution of the model from the different stakeholders’ perspectives; the employer’s as having the legal responsibility for the work environment, and the occupational health service consultants’, being the work environment experts supporting the employers.