bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISA PENGENDALIAN WAKTU DAN BIAYA PADA PROYEK PEMBANGUNAN GEDUNG PERPUSTAK{BIBLIOGRAPHY}AAN MODERN PROVINSI SULAWESI TENGGARA (TAHAP II) ANALYSIS OF TIME CONTROL AND COST IN THE DEVELOPMENT PROJECT OF MODERN LIBRARY PROVINCE OF SOUTH SULAWESI (STAGE II)
Authors: Hardiana Admin , Simatupang Minson , Kadir Abdul
Published: September 2024

Analisa pengendalian waktu dan biaya proyek sebaiknya dilakukan pada masa perencanaan dengan menggunakan analisis metode-metode ilmiah sehingga pada saat pelaksanaan pekerjaan selesai tepat waktu. Penelitian ini menganalisis masalah waktu dan biaya dengan studi kasus "Pembangunan Gedung Perpustakaan Modern Tahap II" dimana pada pembangunan tahap I mengalami keterlambatan disebabkan jadwal pelaksanaan atau time schedule yang digunakan tidak dianalisis sesuai dengan metode lintasan kritis sehingga jumlah sumber daya tidak diperhitungkan dengan cermat disamping itu pada pembangunan di tahap II ini, konsultan perencana hanya membuat gambar rencana kerja, perhitungan biaya pelaksanaan dan spesifikasi teknis. Untuk menghindari keterlambatan pekerjaan seperti pada pembangunan tahap I maka peneliti mencoba melakukan analisis pengendalian waktu dan biaya proyek pada masa perencanaan dengan menggunakan metode jalur kritis dan metode percepatan dimana analisis dimulai dengan mengidentifikasi pekerjaan yang berada pada jalur kritis yang dianalisis dengan Critical Path Method (CPM) untuk mendapatkan cost slope dengan data yang diperoleh dari konsultan perencana kemudian dilakukan percepatan dengan menggunakan metode crash project sehingga diperoleh percepatan waktu dan optimalisasi biaya proyek. Dalam penelitian ini penulis melakukan crashing project 3 simulasi yaitu penambahan 1 jam, 2 jam dan 4 jam kerja sehingga diperoleh hasil analisis yaitu untuk penambahan 1 jam diperoleh pengurangan biaya Rp 88.339.543,70 dari total biaya Rp 29.342.351.124,70 menjadi Rp 29.254.011.581,00 dengan pengurangan waktu 28 hari dari waktu normal 300 hari menjadi 272 hari. Penambahan 2 jam diperoleh pengurangan biaya Rp 114.425.012,25 dari total biaya pekerjaan Rp 29.342.351.124,70 menjadi Rp. 29.227.926.112,45 dengan pengurangan waktu pekerjaan 51 hari dari waktu normal 300 hari menjadi 249 hari. Penambahan 4 jam diperoleh  pengurangan  biaya Rp. 172.809.017,82 dari total biaya  pekerjaan  Rp 29.342.351.124,70  menjadi Rp. 29.169.542.106,88  dengan pengurangan durasi pekerjaan waktu 87 hari dari waktu normal 300 hari menjadi 213 hari. Dalam penelitian ini direkomendasikan sebaiknya percepatan pekerjaan dilaksanakan dengan tambahan waktu kerja maksimal 2 jam yang bertujuan untuk menghindari faktor kelelahan tenaga kerja karena berdampak pada hasil produktifitas dan efektifitas.Kata Kunci:  CPM, Crashing project waktu dan biaya
PERBAIKAN ARUS PROSES PADA FUNGSI YANG TERKAIT DALAM MENINGKATKAN EFEKTIFITAS MANAJEMEN RANTAI SUPLAI PADA FUNGSI PENGADAAN : STUDI KASUS DI DIVISI MANUFAKTUR PERALATAN KAMAR MANDI (PLUMBING-FITTING) PT. SURYA TOTO INDONESIA TBK
Authors: HAKIM ANDI
Published: September 2024

Ada suatu latar belakang di industri Plumbing-Fitting dari PT. Surya Toto indonesia Tbk yang diambil selama periode 3 tahun yaitu 2017 – 2019. Dari data yang terkumpul ternyata pada setiap tahunnya ada terjadi permasalahan yang selalu berulang terlihat dengan arus proses dan efektifitas rantai suplai dari fungsi Departemen PPIC, QC Incoming, QC Customer (Vendor) dan Warehouse Incoming yang mana permasalahan tersebut selalu saja ada pada tahun-tahun tersebut. Peneliti mencoba mendekati masalah tersbut dengan pendekatan yang menggunakan tools atau alat secara berurutan yang antara lain yaitu: Business Process Improvement, Nominal Group Technique, Diagram Pareto, Diagram Fishbone, Analisis Hirarki Proses, Long Term Relationship, yang mana pada gilirannya solusi dari alat manajemen tersebut akan dapat diaplikasikan pada sistem SAP. Keberhasilan pendekatan arus proses dan manajemen rantai suplai yang dilakukan disini akan mempengaruhi efektifitas fungsi pengadaan dengan terjadinya perbaikan pada konsep 4 tepat yaitu tepat waktu, tepat jumlah, tepat harga, tepat mutu dengan demikian maka, 4 permasalahan yang selalu berulang terjadi pada periode 2017 -2019 itu akan menjadi menurun dan berpotensi hilang. Adapun perbaikan arus proses dapat dilakukan dengan penambahan Field pada sistem SAP dan hal tersebut sangat bermanfaat bagi Departemen PPIC dan Marketing, sementara untuk perbaikan atas 3 klaim dari Departemen QC Incoming, QC Customer (Vendor) dan Warehouse Incoming dengan membuat T-Code Detail Claim Information yang nantinya pada claim requestion akan muncul menjadi Summary Compalin Report pada sistem SAP sehingga menggantikan proses kerja dengan software manual. Selanjutnya hubungan fungsi Procurement dengan 4 masalah tersebut diatas bahwa hubungannya dapat ditingkatkan dengan memperbaiki masalah cancel schedule finish goods dan menurunkan 3 klaim lainnya
PENGARUH TINGKAT KERUSAKAN JALAN, PERGERAKAN LALU LINTAS, DAN KECEPATAN KENDARAAN TERHADAP TINGKAT KECELAKAAN DI RUAS JALAN PUNGGALUKU-ANDOOLO KABUPATEN KONAWE SELATAN
Authors: Harto Harto
Published: September 2024

The purpose of this study was to determine and analyze the effect of the level of road damage, daily traffic and vehicle speed on accident rates on the Puunggaluku-Andoolo road section. This type of research is explanatory, descriptive-quantitative in nature. The population in this study were all people who crossed or used the Punggaluku-Andoolo road using two wheels, four wheels and trucks. The sample set in this study is the Punggaluku-Andoolo road section that is divided into 20 observation points. Each variable is analyzed by a different method, the variable level of road damage is analyzed using the PCI method, the variable traffic flow uses the daily traffic (LHR) method while the vehicle speed variable is measured by using a stopwatch tool to get the average speed of each each segment was observed, which was then analyzed using the SPSS 26.0 IMB program in linear regression to see its effect on the vehicle accident rate variable.The results of this research are: (1). The level of damage to the roads in the Pungguluku-Andoolo Road, Konawe Selatan district, on average of the 20 segments observed was in the range of 54.3 values based on the Moderate categorized PCI analysis, while for the daily traffic volume analysis of 788 junior high schools that passed , and an average vehicle speed of 63.98 km / hour; (2). The variable road damage has a negative and not significant effect on the level of vehicle accidents on the Punggaluku-Andoolo road section in Konawe Selatan district; (3) The variable of the movement of traffic flow has a negative but significant effect on the level of vehicle accidents on the Punggaluku-Andoolo road section in Konawe Selatan regency; (4). Vehicle speed variable has a positive but not significant effect on the level of vehicle accidents on the Punggaluku-Andoolo road section in Konawe Selatan district.
PENGARUH KESWADAYAAN TERHADAP PENYELESAIAN RUMAH TIDAK LAYAK HUNI PADA PROGRAM BANTUAN STIMULAN PERUMAHAN SWADAYA DI KOTA KENDARI
Authors: Natsir1 Baso Amrin , Magribi1 La Ode Muhammad , Kadir1 Abdul
Published: September 2024

Salah satu upaya pemerintah dalam mewujudkan rumah layak huni yaitu dengan menyelenggarakan kebijakan Bantuan Stimulan Perumahan Swadaya yang selanjutnya disingkat BSPS yaitu bantuan pemerintah berupa stimulan bagi masyarakat berpenghasilan rendah untuk meningkatkan keswadayaan dalam bentuk uang tunai, tenaga kerja dan bahan bangunan untuk pembangunan dan peningkatan kualitas rumah beserta prasarana, sarana, dan utilitas umum. Metode yang digunakan dalam penelitian ini adalah metode kuantitatif deskriptif.Hasil penelitian dalam pengujian hipotesis menunjukan bahwa keswadayaan uang, bahan dan tenaga berpengaruh signifikan secara bersama-sama dalam penyelesaian pembangunan rumah tidak layah huni di Kota Kendari dengan tingkat pengaruh keswadayaan uang sebesar 80%, Keswadayaan bahan 13,33% serta keswadayaan tenaga sebesar 6,67%.Kata Kunci: Keberhasilan Program, Keswadayaan, BSPS
DIGITASI KAWASAN PERMUKIMAN KUMUH KECAMATAN ABELI KOTA KENDARI
Authors: Husain Fajerullah Akbar , Ngii Edward , Adzikin Muhammad Thahir
Published: September 2024

Uncontrolled population growth, especially in urban areas, has a negative impact on social problems, one of which is the emergence of enclaves. Development and increasing population growth rates always bring changes, one of which is a change in the level of environmental quality. Rapidly growing population growth has implications for increasing space requirements for shelter and other facilities.The quality of the rapidly changing housing environment is encouraging managers and policy makers in the city to carry out monitoring and evaluation. The problem then is that measurement and evaluation require high costs, a lot of energy, and a relatively long time, given the very crowded and bustling city environment. Therefore we need a method that is able to overcome these obstacles.Characteristics of average slum in terms of indicators of building presentation, environmental accessibility, environmental drainage, drinking water services, water and sewage management, abeli village 15%, anggalomelai village 46%, continental nirae village 40%, bungkutoko village 52.80%, lapulu 27 %, pudai 65.62% and talia 32% and based on the slum table of abeli 15% are declared not slum, anggalomelai kelurahan 46% moderate slum, continental nirae village 40% mild slum, bungkutoko kelurahan 52.80% moderate slum, 27% mild slum kelurahan , pudai 65.62% moderate slums and 32% mild talia kelurahanEstablishment of a geographic information system based on the location map of the slum of Abeli sub-district. The geographic information system can display information and location of Abeli sub-district slum with five indicators namely buildings, environmental accessibility, environmental drainage, water service play and wastewater management which are spread in the Abeli sub-district and the establishment of a map-based geographic information system that can be utilized to search slum based on the area can be displayed on the map location Abeli district.keywords: slums, settlements, digitizing
ANALISIS TINGKAT KECELAKAAN LALU LINTAS BERDASARKAN TIPOLOGI KECELAKAAN PADA JALAN NASIONAL RUAS JALAN WAWOTOBI - POHARA
Authors: Syarifuddin Syarifuddin , Magribi La Ode Muh , Putra Adris Ade
Published: September 2024

Kecelakaan lalu-lintas adalah suatu peristiwa di jalan yang tidak diduga dan tidak disengaja melibatkan kendaraan dengan atau tanpa pengguna jalan lain yang mengakibatkan korban manusia dan/atau kerugian harta benda. Tujuan dari penelitian ini adalah untuk mengetahui tingkat kecelakaan lalu lintas berdasarkan tipologi kecelakaan pada jalan nasional ruas jalan Wawotobi-Pohara. Penelitian ini menggunakan metode pendekatan secara kualitatif, dimana penelitian kualitatif merupakan penelitian yang digunakan untuk menyelidiki, menemukan, menggambarkan dan menjelaskan kualitas atau keistimewaan dari pengaruh sosial yang tidak dapat dijelaskan, diukur, dan digambarkan melalui pendekatan kuantitatif. Pengumpulan data dalam penelitian ini adalah dengan cara studi literatur.Hasil dari penelitian ini didapatkan bahwa : (1) tingkat kecelakaan tertinggi pada 2018 sebesar 64 kecelakaan; (2) keparahan dengan jumlah terbanyak terjadi pada tahun 2016 dengan orang meninggal berjumlah 18 orang; (3) Kecelakaan lalu lintas berdasarkan jenis kelamin didominasi oleh laki-laki dengan jumlah rata-rata persentasi kecelakan tiap tahunya sebesar tahunya sebesar 79,5% untuk laki-laki dan 20,5% (4) Kecelakaan lalu lintas berdasarkan waktu kejadian pada tahun 2015 sampai dengan 2019 terjadi paling banyak pada pukul 14.00-22.00; (5) kecelakaan lalu lintas berdasarkan jenis tabrakannya didominasi oleh tabrak depan; (5) Kecelakaan berdasarkan penyebabnya disebabkan oleh tiga faktor yaitu manusia , kendaraan dan jalan (6) ruas jalan dan kecematan yang berada di ruas jalan Wawotobi-Pohara tidak memilki daerah rawan kecelakaan; (7) upaya penanggulangan kecelakaan dan perbaikan jalan dengan melakukan perbaikan geomterik jalan, pemasangan marka jalan dan perbaikan struktur jalanKata Kunci: kecelakaan, tipologi, lalu lintas
KAJIAN LAIK FUNGSI JALAN (Studi Kasus pada Ruas Jalan Orinunggu Jalan Provinsi Sulawesi Tenggara)
Authors: Taziruddin Taziruddin , Putra Adris Ade , Kadir Abdul
Published: September 2024

Ruas Jalan Orinunggu merupakan Jalan Provinsi Sulawesi Tenggara yang terletak di Kota Kendari yang sebagian besar belum pernah teruji laik fungsi jalan. Data dari Polda Sulawesi Tenggara tahun 2019 mengatakan bahwa jumlah peristiwa kecelakaan tiap bulannya rata-rata 15 kejadian, Dalam mewujudkan kondisi suatu ruas jalan yang memenuhi keselamatan bagi penggunanya juga mengurangi tingkat risiko kecelakaan yang sering terjadi di jalan raya, melalui uji laik fungsi jalan dapat mengetahui kondisi suatu ruas jalan apakah telah memenuhi persyaratan teknis kelaikan dan persyaratan administrasi sehingga dapat mewujudkan jalan yang sesuai dengan ketentuan keselamatan.Tujuan dari penelitian ini adalah: (1) mengetahui kategori kelaikan fungsi teknis Ruas Jalan Orinunggu Km 0 - Km 3,15 berdasarkan parameter perencanaan laik fungsi jalan, dan (2) Mengetahui tindak lanjut yang perlu dilakukan apabila Ruas Jalan Orinunggu Km 0 - Km 3,15 belum memenuhi kategori kelaikan jalan secara teknis. Pengambilan data di lapangan menggunakan Metode Uji dengan cara pengamatan dan pengukuran kondisi ruas jalan Orinunggu Kendari Km 0 - Km 3,15 terhadap standar teknis, meliputi: (1) teknis geometrik jalan; (2) teknis struktur perkerasan jalan; (3) teknis struktur bangunan pelengkap jalan; (4) teknis pemanfaatan bagian - bagian jalan; (5) teknis penyelenggaraan manajemen dan rekayasa lalu lintas; (6) teknis perlengkapan yang terkait langsung dengan pengguna jalan maupun yang tidak terkait langsung dengan pengguna jalan. Dari hasil uji lapangan dilakukan analisis laik fungsi jalan secara teknis menggunakan Metode Evaluasi yaitu membandingkan dengan parameter perencanaan laik fungsi jalan (Peraturan Menteri Nomor: 11/PRT/M/2010 dan Panduan Teknis Pelaksanaan Laik Fungsi Jalan Tahun 2012).Hasil analisis kelaikan fungsi jalan pada ruas jalan Orinunggu – Kendari Km 0 - Km 3,15 yaitu laik fungsi bersyarat dengan rekomendasi (LS). Tindak lanjut yang dilakukan pada ruas jalan Orinunggu Km 0 – Km 3,15 yaitu dengan memberikan rekomendasi sesuai dengan kondisi fisik lingkungan jalan yang dapat memenuhi ketentuan keselamatan, kelancaran, ekonomis, dan ramah lingkungan.Kata kunci: Ruas Jalan Orinunggu, Laik Fungsi Jalan, Standar Teknis
Efektivitas Program Penyuluhan Pencegahan Penyakit Septichaemia epizootica pada Sapi Ongole di Kecamatan Tana Righu Kabupaten Sumba Barat Provinsi, Nusa Tenggara Timur
Authors: Fangidae Marlince Margaretha Yenny , Gamaruddin Gamaruddin
Published: September 2024

Mortalitas dan morbiditas ternak di Kecamatan Tana Righu disebabkan oleh penyakit ternak, terutama penyakit menular, kurangnya pengetahuan peternak, koordinasi yang buruk, dan layanan penyuluhan yang kurang optimal. Penelitian tentang pencegahan penyakit Septicaemia epizootica (SE) di Tana Righu bertujuan untuk (1) mengevaluasi program penyuluhan pencegahan SE, (2) menilai pengetahuan peternak tentang pencegahan SE pada sapi Ongole, dan (3) mengkaji hubungan antara karakteristik individu dengan pengetahuan tentang pencegahan SE. Dengan metode sampling jenuh, 43 responden dari Desa Lokory, Lolo Tana, dan Lolo Wano berpartisipasi. Analisis data menggunakan Spearman Rank. Hasil menunjukkan efektivitas program penyuluhan sebesar 92,25%, pengetahuan peternak sebesar 82,63% (sangat tinggi), dan hubungan karakteristik individu dengan pengetahuan SE diantaranya: usia, lama beternak, dan jumlah tanggungan keluarga positif tetapi tidak signifikan;  pendidikan negatif dan signifikan, sementara jumlah ternak positif dan sangat signifikan.
Fine mapping-based multi-omics analysis interprets the gut-lung axis function of SGLT2 inhibitors
Authors: Yuan Fengqin , Zhang Tianlong , Jia Sixiang , Zhao Jianqiang , Wan Binbin , Liu Gang
Published: September 2024

Background

Currently, Sodium-glucose cotransporter 2 (SGLT2) inhibitors demonstrate additional effects beyond glucose control on the gut microbiota and circulating metabolites. The gut microbiota and metabolites have been found to be useful in elucidating potential biological mechanisms of pulmonary diseases. Therefore, our study aims to investigate the effects of gut microbiota and metabolites mediating SGLT2 inhibition in 10 pulmonary diseases through Mendelian randomization (MR) research.

Methods

We conducted a two-sample, two-step MR study to assess the association between SGLT2 inhibition and 10 pulmonary diseases and to investigate the mediating effects of gut microbiota and metabolite. Gene-fine mapping and annotation of mediators by FUMA and Magma analyses were performed, and causal associations of mapped genes with diseases were assessed by muti-omics MR analyses. Possible side effects of SGLT2 inhibition were assessed by PheWAS analysis.

Results

SGLT2 inhibition was linked to a reduced risk of T2DM, Interstitial lung disease (ILD), Pneumoconiosis, Pulmonary tuberculosis, and Asthma(OR=0.457, 0.054, 0.002, 0.280, 0.706). The family Enterobacteriaceae and order Enterobacteriales were associated with SGLT2 inhibition and ILD(95% CI:0.079–0.138). The family Alcaligenaceae and X-12719 were linked to pneumoconiosis (95% CI: 0.042–0.120, 0.050–0.099). The genus Phascolarctobacterium was connected to pulmonary tuberculosis (95% CI: 0.236–0.703).The degree of unsaturation (Fatty Acids), ratio of docosahexaenoic acid to total fatty acids, and 4-androsten-3beta,17beta-diol disulfate 2, were associated with asthma(95% CI: 0.042–0.119, 0.039–0.101, 0.181–0.473). Furthermore, Fuma and Magma analyses identified target genes for the four diseases, and proteomic MR analysis revealed six overlapping target genes in asthma. PheWAS analysis also highlighted potential side effects of SGLT2 inhibition.

Conclusions

This comprehensive study strongly supports a multi-omics association between SGLT2 inhibition and reduced risk of interstitial lung disease, tuberculosis, pneumoconiosis, and asthma. Four identified gut microbiota, four metabolites, sixteen metabolic pathways, and six target genes appear to play a potential role in this association. The results of the comprehensive phenome-wide association analysis also identified the full effect of SGLT2 inhibitors.
Pulmonary artery in situ thrombosis due to patent ductus arteriosus: a case report
Authors: Wang Yin , Rong Chunyan , Lu Ming , Zhang Weihua
Published: September 2024

Background

Pulmonary Artery in situ Thrombosis (PAIST) refers to a thrombus forming within the pulmonary arterial system, distinct from an embolus originating from elsewhere in the body (e.g., the deep veins of the lower extremities) and traveling to the lungs where it lodges and forms.

Case presentation

We present a case of PAIST caused by the arterial ductus arteriosus. The patient primarily presented with dyspnea, and the chest pain dichotomy Computed Tomography Angiography(CTA) suggested that a nodular low-density filling defect was seen in the lumen of the left pulmonary artery trunk. Initially, pulmonary embolism (PE) was suspected. However, upon reevaluation of the imaging, it became apparent that the patient's pulmonary artery obstruction was closely associated with the ductus arteriosus. After admission, the patient was treated with sodium ampicillin (2.0 g Q12H) for infection, heparin sodium (5,000 IU Q12H) for anticoagulation, and metoprolol succinate extended-release tablets (23.75 mg QD) to correct cardiac remodeling, among other treatments. Subsequently, the patient underwent a cardiac surgery involving the ligation of the arterial duct, resection of pulmonary artery lesions, and open-heart surgery with extracorporeal circulation support. Postoperative examination of the pulmonary artery mass indicated coagulation tissue. The final diagnosis was “PAIST”.

Conclusion

Both PAIST and PE manifest as low-density filling defects in the pulmonary arteries. However, due to the relative unfamiliarity with PAIST, such findings are often initially attributed to PE.
Independent factors for the development of vasoplegic syndrome in patients undergoing coronary artery bypass surgery
Authors: Palm Constantin L. , Baumhove Lukas , Pabst Simon , Guenther Ulf , Book Malte , Chaduneli Onise , Martens Andreas , Mellert Friedrich , Dewald Oliver
Published: September 2024

Objective

Vasoplegic syndrome remains a common complication of cardiac surgery. It has serious implications for the healthcare system and individual patients, as it leads to rising healthcare costs and higher mortality. A better understanding of factors triggering vasoplegic syndrome is essential for the development of effective prevention strategies. We aimed to identify clinical characteristics and intraoperative parameters associated with the development of vasoplegic syndrome in coronary artery bypass graft surgery and the influence of vasoplegia on outcome.

Methods

We retrospectively analyzed the data of all patients who underwent isolated coronary artery bypass graft surgery or coronary artery bypass graft surgery combined with atrial appendage occlusion, using the heart-lung machine at our institution from 04/2019 to 12/2020. Vasoplegic syndrome was defined as MAP ≤60 mmHg and norepinephrine equivalence dosage of ≥0.2 μg/kg/min with a central venous saturation ≥60% within 2 days from surgery.

Results

Of 647 patients included in this study, 72 (11.1%) developed vasoplegic syndrome. Patients experiencing vasoplegia had longer stay in ICU, more frequently underwent tracheostomy and suffered more often from pneumonia. The duration of extracorporeal circulation, intraoperative application of platelet concentrates and usage of cold crystalloid cardioplegia (Bretschneider) independently predicted development of vasoplegic syndrome.

Conclusions

Even in relatively low-risk cardiac surgery, vasoplegic syndrome is a common complication and was associated with serious adverse effects. The use of warm blood cardioplegia (Calafiore) seems to be safer than cold crystalloid cardioplegia (Bretschneider) and might be preferable in patients that are vulnerable to the consequences of vasoplegic syndrome.
VCAM1: an effective diagnostic marker related to immune cell infiltration in diabetic nephropathy
Authors: Deng Yuanyuan , Zhang Sai , Luo Zheng , He Pengfei , Ma Xinyu , Ma Yu , Wang Jing , Zheng Liyang , Tian Ni , Dong Shaoning , Zhang Xingkun , Zhang Mianzhi
Published: September 2024

Introduction

The role of immune cells in the pathogenesis and advancement of diabetic nephropathy (DN) is crucial. The objective of this study was to identify immune-cell-related biomarkers that could potentially aid in the diagnosis and management of DN.

Methods

The GSE96804 dataset was obtained from the Gene Expression Omnibus (GEO) database. Then, screen for intersections between differentially expressed genes (DEGs) and immune-related genes (IRGs). Identify core genes through protein-protein interaction (PPI) networks and the Cytoscape plugin. Subsequently, functional enrichment analysis was conducted. In addition, ROC analysis is performed to accurately identify diagnostic biomarkers. Apply the CIBERSORT algorithm to evaluate the proportion of immune cell infiltration. Finally, the mRNA, protein, and immunofluorescence expression of the biomarker was validated in the DN rat model.

Results

The study yielded 74 shared genes associated with DN. Enrichment analysis indicated significant enrichment of these genes in focal adhesion, the humoral immune response, activation of the immune response, Cytokine-cytokine receptor interaction, and IL-17 signaling pathway. The optimal candidate gene VCAM1 was identified. The presence of VCAM1 in DN was further validated using the ROC curve. Analysis of immune cell infiltration matrices revealed a high abundance of monocytes, naïve B cells, memory B cells, and Macrophages M1/M2 in DN tissues. Correlation analysis identified one hub biomarker associated with immune-infiltrated cells in DN. Furthermore, our findings were validated through in vivo RT qPCR, WB, and IF techniques.

Conclusions

Our research indicates that VCAM1 is a signature gene associated with DN and is linked to the progression, treatment, and prognosis of DN. A comprehensive examination of immune infiltration signature genes may offer new perspectives on the clinical diagnosis and management of DN.
A cost-of-illness study of eosinophilic esophagitis in Italy: assessing direct and indirect costs
Authors: Fortunato Agostino , Antonini Debora , Savarino Edoardo Vincenzo , Racca Francesca , Penagini Roberto , Fanelli Francesca , Saab Jean Pierre , Cipriani Filippo , Giodice Roberta , Rumi Filippo , Cicchetti Americo
Published: September 2024

Background

Eosinophilic esophagitis (EoE) is a chronic and progressive type 2 inflammatory disease affecting the esophagus. Its prevalence has increased in recent years due to increased awareness, evolving clinical guidelines, and heightened sensitivity among healthcare professionals managing the condition. The exact causes behind EoE’s development remain unknown, and its clinical presentation varies, often leading to significant diagnostic delays depending on the age at which symptoms manifest. Consequently, achieving long-term disease control through heightened awareness becomes imperative. EoE generates a significant clinical burden, resulting in substantial economic consequences for patients, healthcare systems, and society. This study aimed to assess the economic and social impacts on EoE patients within the Italian context.

Methods

A cost-of-illness analysis was conducted from two perspectives: the National Health System (NHS) and the societal perspective. This analysis encompassed direct healthcare, indirect healthcare, and non-healthcare costs. Data were collected and assessed through a survey administered to a panel of expert clinicians and EoE-affected patients.

Results

Managing EoE incurs a significant burden on healthcare systems, amounting to €6,852.28 per patient per year. The primary cost component appears to be direct costs, comprising 60.73% of the total cost per patient for this condition, while indirect costs contribute to 29.68% of the overall management expenses.

Conclusion

This analysis underscores a substantial financial burden on both the healthcare system and patients affected by eosinophilic esophagitis. It emphasizes the imperative need for a continuous and combined effort from clinicians, patients, and families to promptly recognize symptoms and adaptive behavior to mitigate diagnostic delays.
Into the storm: the imbalance in the yin-yang immune response as the commonality of cytokine storm syndromes
Authors: Armstrong Amy , Tang Yuting , Mukherjee Neelam , Zhang Nu , Huang Gang
Published: September 2024

There is a continuous cycle of activation and contraction in the immune response against pathogens and other threats to human health in life. This intrinsic yin-yang of the immune response ensures that inflammatory processes can be appropriately controlled once that threat has been resolved, preventing unnecessary tissue and organ damage. Various factors may contribute to a state of perpetual immune activation, leading to a failure to undergo immune contraction and development of cytokine storm syndromes. A literature review was performed to consider how the trajectory of the immune response in certain individuals leads to cytokine storm, hyperinflammation, and multiorgan damage seen in cytokine storm syndromes. The goal of this review is to evaluate how underlying factors contribute to cytokine storm syndromes, as well as the symptomatology, pathology, and long-term implications of these conditions. Although the recognition of cytokine storm syndromes allows for universal treatment with steroids, this therapy shows limitations for symptom resolution and survival. By identifying cytokine storm syndromes as a continuum of disease, this will allow for a thorough evaluation of disease pathogenesis, consideration of targeted therapies, and eventual restoration of the balance in the yin-yang immune response.
Ethical governance and implementation paths for global marine science data sharing
Authors: Zhu Mingting , Zhang Weibin , Xu Chongli
Published: September 2024

While global marine science data sharing plays a crucial role in advancing scientific research and knowledge dissemination, it also brings with it two major ethical conflicts: those between individual interests and the public interest and those between data privacy and data sharing, which are rooted in conflict of interest. In the context of globalization and rapid technological progress, current ethical governance frameworks are inadequate in regulating these conflicts. On the one hand, governance cannot meet the needs of rapidly evolving technologies; on the other hand, the complexity and diversity of stakeholders involved in governance make it more difficult. By introducing the theory of legal hierarchy and innovatively proposing the theory of ethical hierarchy, which classifies the ethical principles to be followed according to their importance and applicability, the priority and scope of application between different ethical principles can be clearly defined so that various stakeholders can make the right ethical choices. Using research methods such as literature review, case studies, and comparative analysis, we propose to construct an ethical governance framework that consists of a stakeholder consultation and communication network in terms of governance structure and clarifies the ethical responsibilities of each party in data sharing in terms of governance measures. This framework not only provides a platform for communication and cooperation among different stakeholders but also helps to balance the interests of different stakeholders and improve the level and extent of global marine science data sharing.
Rapid de-escalation of anti-MRSA therapy guided by S. aureus nares screening for patients with pneumonia: protocol of a randomized controlled trial (SNAP study)
Authors: De Vita Elda , Segala Francesco Vladimiro , Cavallin Francesco , Guido Giacomo , Frallonardo Luisa , Cotugno Sergio , Pellegrino Carmen , Di Gennaro Francesco , Saracino Annalisa
Published: September 2024

Introduction

The current Infectious Disease Society of America and American Thoracic Society (IDSA/ATS) guidelines recommend linezolid or vancomycin as an empiric treatment for methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in hospitalized patients with specific risk factors,. A nasal PCR-assay for MRSA, with its high negative predictive value, can guide a rapid antibiotic de-escalation avoiding unnecessary anti-MRSA treatment. The indiscriminate use of these drugs has contributed to the emergence of resistant S. aureus strains leading to adverse effects without any survival benefit, increasing hospital stays and associated costs. Aim of the study is the use of this diagnostic tool to reduce empirical anti-MRSA treatment duration in pneumonia, shortening antimicrobial therapy days while measuring in-hospital mortality, length of stay and adverse drug event incidence.

Methods

It is a prospective, randomized single-center controlled trial planned to be conducted in the Azienda Consorziale Policlinico di Bari. The research project will have a duration of 12 months following the approval of the Ethical Committee of the University of Bari. The minimum sample size is 38 patients per group, for a total of 76 subjects, calculated assuming a standard deviation of 10, a power of 90%, a type I error of 5% and a 10% drop-out rate. We will enroll eligible patients ensuring their evidence-based management according to guidelines, we will perform a nasal swab for MRSA in patients in the experimental group and discontinue the empirical anti-MRSA therapy if the nasal swab result is negative. For both arms, follow-up visits will be on day 2, 5, 7, 14, and 28 relatives to the enrollment visit (day 0). Data will be collected on the clinical course of pneumonia and laboratory tests.

Discussion

Our study will provide evidence on the duration (in days) of the antibiotic intake as a primary outcome of the study. Secondary outcome measures include in-hospital mortality, the length of stay and days of mechanical ventilation (in VAP), and the incidence of adverse events related to the administration of the therapy.

Clinical trial registration

https://classic.clinicaltrials.gov/ct2/show/NCT06238297, identifier NCT06238297.
The neurocognitive mechanism linking temperature and humidity with miners’ working memory: an fNIRS study
Authors: Tian Chenning , Li Hongxia , Tian Shuicheng , Tian Fangyuan , Yang Hailan
Published: September 2024

Background

In China’s coal mines, employees work in environments reaching depths of 650 m, with temperatures around 40°C and humidity levels as high as 90%, adversely affecting their health, safety capabilities, and cognitive functions, especially working memory. This study aims to explore different temperature and humidity conditions’ impact on neurocognitive mechanisms to enhance occupational health and safety.

Methods

This study, conducted between June and August 2023, with 100 coalmine workers from the Hongliulin Mining Group, utilized functional near infrared spectroscopy (fNIRS) and short-term visual memory tasks to evaluate the effects of high temperatures and humidity on working memory by monitoring activity in the cerebral cortex. Behavioral data, and neurophysiological data were analyzed using Tukey’s HSD for significant differences and multiple regression to explore the impact of temperature and humidity. The β-values of Oxy-Hb for different regions of interest were calculated using General liner model (GLM), and the activation maps were plotted by NIRS_KIT.

Results

High temperature and humidity (Condition IV) significantly depressed reaction times and working memory compared to other conditions, with temperature having a more pronounced impact than humidity on these cognitive measures (p < 0.05). Oxy-Hb concentration increased notably under Condition IV, emphasizing temperature’s influence on brain oxygen levels. ROI analysis revealed varied brain activation patterns. The activation of ROI A and B (prefrontal cortex) increased with the increase of temperature and humidity, while ROI C (supplementary motor area) was less sensitive to temperature, indicating the complex influence of environmental factors on brain function.

Conclusion

This study highlights the important effects of temperature and humidity on cognitive performance and brain function, highlighting the need to optimize the environment of miners’ sites to improve productivity and safety.
The link between serum cotinine levels and gallstones prevalence in adults: a cross-sectional analysis using NHANES data (2017–2020)
Authors: Liu Xin , Zhang Zheng , Wang Haoran , Faisal Shah , He Meng , Tai Sheng , Lin Yujia
Published: September 2024

Background

Gallstones represent a prevalent health issue globally, resulting in significant annual healthcare costs. While tobacco exposure is recognized for its association with numerous diseases, its correlation with gallstones remains contentious. Serum cotinine, a metabolite of nicotine, serves as a widely utilized indicator for assessing tobacco exposure. Crucially, no research has yet examined the association between serum cotinine levels and the gallstones.

Methods

This study is designed as a cross-sectional analysis, utilizing data from the NHANES public database. The relationship between serum cotinine levels and gallstones was analyzed using multinomial logistic regression models and smooth curve fitting. Subgroup analyses and interaction tests were performed to examine the potential contributions of different populations and covariates to the findings.

Results

A total of 5,856 participants were included in this study. After adjusting for relevant covariates, the multiple logistic regression model results indicated that for each unit increase in serum cotinine concentration above 0.29 ng/mL, there was a 29% increase in the prevalence of gallstones. Furthermore, smooth curve fitting analysis revealed a positive correlation between these variables. These findings underscore the impact of tobacco exposure on gallstone prevalence.

Conclusion

This study demonstrates a positive correlation between tobacco exposure, as measured by serum cotinine levels, and the prevalence of gallstones, thus adding to the body of existing research on this relationship.
Random measurement and prediction errors limit the practical relevance of two velocity sensors to estimate the 1RM back squat
Authors: Warneke Konstantin , Skratek Josua , Wagner Carl-Maximilian , Wirth Klaus , Keiner Michael
Published: September 2024

Introduction

While maximum strength diagnostics are applied in several sports and rehabilitative settings, dynamic strength capacity has been determined via the one-repetition maximum (1RM) testing for decades. Because the literature concerned several limitations, such as injury risk and limited practical applicability in large populations (e.g., athletic training groups), the strength prediction via the velocity profile has received increasing attention recently. Referring to relative reliability coefficients and inappropriate interpretation of agreement statistics, several previous recommendations neglected systematic and random measurement bias.

Methods

This article explored the random measurement error arising from repeated testing (repeatability) and the agreement between two common sensors (vMaxPro and TENDO) within one repetition, using minimal velocity thresholds as well as the velocity = 0 m/s method. Furthermore, agreement analyses were applied to the estimated and measured 1RM in 25 young elite male soccer athletes.

Results

The results reported repeatability values with an intraclass correlation coefficient (ICC) = 0.66–0.80, which was accompanied by mean absolute (percentage) errors (MAE and MAPE) of up to 0.04–0.22 m/s and ≤7.5%. Agreement between the two sensors within one repetition showed a systematic lower velocity for the vMaxPro device than the Tendo, with ICCs ranging from 0.28 to 0.88, which were accompanied by an MAE/MAPE of ≤0.13 m/s (11%). Almost all estimations systematically over/ underestimated the measured 1RM, with a random scattering between 4.12% and 71.6%, depending on the velocity threshold used.

Discussion

In agreement with most actual reviews, the presented results call for caution when using velocity profiles to estimate strength. Further approaches must be explored to minimize especially the random scattering.
The effect of 60 days of 6° head-down-tilt bed rest on circulating adropin, irisin, retinol binding protein-4 (RBP4) and individual metabolic responses in young, healthy males
Authors: Ward Kiera , Mulder Edwin , Frings-Meuthen Petra , O’Gorman Donal J. , Cooper Diane
Published: September 2024

Background

Alterations in the circulating concentrations and target-tissue action of organokines underpin the development of insulin resistance in microgravity and gravity deprivation. The purpose of this study was to examine changes in circulating adropin, irisin, retinol binding protein-4 (RBP4), and the metabolic response of healthy young males following 60 days of 6° head-down-tilt (HDT) bed rest, with and without reactive jump training (RJT), to explore links with whole-body and tissue-specific insulin sensitivity. To our knowledge, this is the first time that adropin, irisin, and RBP4 have been studied in HDT bed rest.

Methods

A total of 23 male subjects (29 ± 6 years, 181 ± 6 cm, 77 ± 7 kg) were exposed to 60 days of 6° HDT bed rest and randomized to a control (CTRL, n = 11) or a RJT (JUMP, n = 12) group (48 sessions with ≤4 min total training time per session). Circulating adropin, irisin, and RBP4 were quantified in fasting serum before and after HDT bed rest. A subanalysis was performed a posteriori to investigate individual metabolic responses post-HDT bed rest based on subjects that showed an increase or decrease in whole-body insulin sensitivity (Matsuda index).

Results

There were significant main effects of time, but not group, for decreases in adropin, irisin, Matsuda index, and liver insulin sensitivity following HDT bed rest (p < 0.05), whereas RBP4 did not change. The subanalysis identified that in a subgroup with decreased whole-body insulin sensitivity (n = 17), RBP4 increased significantly, whereas adropin, irisin, and liver insulin sensitivity were all decreased significantly following HDT bed rest. Conversely, in a subgroup with increased whole-body insulin sensitivity (n = 6), liver insulin sensitivity increased significantly after HDT bed rest, whereas adropin, irisin, and RBP4 did not change.

Conclusion

Investigating individual metabolic responses has provided insights into changes in circulating adropin, irisin, RBP4, in relation to insulin sensitivity following HDT bed rest. We conclude that adropin, irisin, and RBP4 are candidate biomarkers for providing insights into whole-body and tissue-specific insulin sensitivity to track changes in physiological responsiveness to a gravity deprivation intervention in a lean male cohort.
Rare occurrence of cryptic 5’ splice sites by downstream 3’ splice site/exon boundary mutations in a heavy-ion-induced egy1-4 allele of Arabidopsis thaliana
Authors: Sanjaya Alvin , Nishijima Ryo , Fujii Yuki , Asano Makoto , Ishii Kotaro , Kazama Yusuke , Abe Tomoko , Fujiwara Makoto T.
Published: September 2024

Pre-mRNA splicing is a fundamental process in eukaryotic gene expression, and the mechanism of intron definition, involving the recognition of the canonical GU (5’-splice site) and AG (3’-splice site) dinucleotides by splicing factors, has been postulated for most cases of splicing initiation in plants. Splice site mutations have played crucial roles in unraveling the mechanism of pre-mRNA splicing in planta. Typically, splice site mutations abolish splicing events or activate one or more cryptic splice sites surrounding the mutated region. In this report, we investigated the splicing pattern of the EGY1 gene in an Ar-ion-induced egy1-4 allele of Arabidopsis thaliana. egy1-4 has an AG-to-AC mutation in the 3′-end of intron 3, along with 4-bp substitutions and a 5-bp deletion in adjacent exon 4. RT-PCR, cDNA cloning, and amplicon sequencing analyses of EGY1 revealed that while most wild-type EGY1 mRNAs had a single splicing pattern, egy1-4 mRNAs had multiple splicing defects. Almost half of EGY1 transcripts showed ‘intron retention’ at intron 3, while the other half exhibited activation of 3’ cryptic splice sites either upstream or downstream of the original 3’-splice site. Unexpectedly, around 8% of EGY1 transcripts in egy1-4 exhibited activation of cryptic 5′-splice sites positioned upstream of the authentic 5’-splice site of intron 3. Whole genome resequencing of egy1-4 indicated that it has no other known impactful mutations. These results may provide a rare, but real case of activation of cryptic 5’-splice sites by downstream 3’-splice site/exon mutations in planta.
Self-appreciation is not enough: exercise identity mediates body appreciation and physical activity and the role of perceived stress
Authors: Shi Linyu , Jiang Lixia , Zhou Song , Zhou Wenbo , Yang Huaqi
Published: September 2024

Introduction

This study explores the relationship between body appreciation and physical activity, focusing on the mediating role of exercise identity and the moderating effect of perceived stress. While individuals with positive body image are generally thought to engage in proactive physical activity, it remains unclear whether this positive attitude necessarily promotes exercise.

Methods

We conducted a short-term longitudinal survey, recruiting 345 college students 28 (100 females, 245 males; Mage = 22.94, SD = 5.99) who completed questionnaires at two-week intervals for a total of three times within four weeks. Body appreciation, exercise identity, perceived stress, and physical activity were measured for the participants separately.

Results

The results demonstrated that body appreciation positively predicted physical activity, exercise identity partially mediated the positive effect of body appreciation on physical activity, and perceived stress played a moderating role in body appreciation and exercise identity.

Discussion

These results highlight the significant role of body appreciation in influencing physical activity through exercise identity, with perceived stress further moderating this relationship. The study underscores the importance of promoting body appreciation and regulating stress to enhance physical activity engagement among college students.
Emotional factors and self-efficacy in the psychological well-being of trainee teachers
Authors: Gilar-Corbi Raquel , Perez-Soto Natalia , Izquierdo Andrea , Castejón Juan-Luis , Pozo-Rico Teresa
Published: September 2024

Introduction

The relationship among emotional intelligence, stress, and self-efficacy is a crucial factor in shaping psychological well-being. It has a significant impact on important areas such as health, academic and professional performance, and overall quality of life.

Methods

Using a hierarchical approach, this study aimed to identify, the specific predictors of psychological well-being, including emotional intelligence, stress, resilience, burnout, and self-efficacy, among higher education students pursuing a bachelor’s degree in education. We also examined gender differences among these predictors. This study involved 338 higher education students pursuing a primary education teaching degree.

Results

The results obtained using the hierarchical regression analysis technique, indicated that the resilience measure, the burnout measure, and the factor of the teacher self-efficacy measure related to self-efficacy in coping with challenges and effectiveness in dealing with change in the educational context, significantly contributed to explaining psychological well-being in the total sample. Furthermore, the predictors of psychological well-being differed between male and female samples.

Discussion

Finally, these findings are discussed in terms of their theoretical and practical implications for improving the training process of future teachers.
Restoration of abnormal sleep EEG power in patients with insomnia disorder after 1Hz rTMS over left DLPFC
Authors: Zhao Xumeng , Liu Jiayi , Shao Ziqiang , Liu Xiaoyang , Wang Zhen , Yuan Kai , Zhang Bingqian , Li Yan , Sheng Xiaona , Zhu Yifei , Guo Yansu
Published: September 2024

Introduction

Hyperarousal has been a significant pathophysiological theory related to insomnia disorder (ID), characterized by excessive cortical activation and abnormal electroencephalogram (EEG) power during daytime or sleep. However, there is currently insufficient attention to the EEG power during rapid eye movement (REM) sleep and different stages of non-rapid eye movement (NREM) sleep. Additionally, whether the abnormal sleep EEG power in ID patients can be restored by repetitive transcranial magnetic stimulation (rTMS) remains unclear.

Methods>

Data of 26 ID patients and 26 healthy controls (HCs) were included in the current observational study. The comparisons of relative power between patients and HCs at baseline in each band of each sleep stage and the changes in patients before and after rTMS treatment were performed. The correlations between relative power and behavioral measures of the patients were also investigated.

Results

Abnormalities in sleep EEG relative power in the delta, beta and gamma bands of the patients were observed in NREM2, NREM3 and REM sleep. Correlations were identified between relative power and behavioral measures in ID group, primarily encompassing sleep efficiency, sleep onset latency and depression scores. Post-treatment improvements in relative power of the delta and beta band were observed in NREM2 sleep.

Discussion

The relative power of sleep EEG exhibited a significant correlation with sleep measures in ID patients, and demonstrated notable differences from HCs across the delta, beta, and gamma frequency bands. Furthermore, our findings suggest that rTMS treatment may partially ameliorate relative power abnormalities in patients with ID.