bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Self-Compassion in the Job Demands-Resources Model, an Explorative Study among Crisis Line Volunteers
Authors: Willems Renate , Drossaert Constance , ten Klooster Peter , Miedema Harald , Bohlmeijer Ernst
Published: September 2021

The job demands-resources (JD-R) model has hardly been studied in volunteer organizations and there is a scarcity of studies evaluating self-compassion as a personal resource within the JD-R model. The present study addresses these gaps in current knowledge, first by examining the applicability of the JD-R model in a crisis line volunteer organization. Second, self-compassion is examined, both in terms of its moderating role on the exhaustion process as well as its role on the motivation process. Structural equation modelling was used for the analyses. The influence on the organizational outcome ‘compassion towards others’ was examined using a multiple regression analysis. The results showed that the JD-R model has an acceptable fit on this sample and supports the central assumption that exhaustion and motivation are two independent but related processes. This study provides evidence that self-compassion is a valuable addition to the JD-R model, as it has an indirect effect on both processes, and increases the explained variance in compassion towards others by 7% through the exhaustion process and by 3% through the motivational process. These findings point to the importance of focusing on self-compassion in training and supervision in volunteer organizations.
Long-Term Sequelae of Frostbite—A Scoping Review
Authors: Regli Ivo B. , Strapazzon Giacomo , Falla Marika , Oberhammer Rosmarie , Brugger Hermann
Published: September 2021

Frostbite is tissue damage caused by freezing temperatures and constitutes an important cause of morbidity in cold climate zones and high altitude. The direct effects of sub-zero temperatures lead to tissue freezing, electrolyte shifts and pH alterations, microvascular damage, and eventually to cell death. Upon rewarming, inflammatory reperfusion injury and thrombosis may lead to further tissue damage. Several studies and various case reports show that many patients suffer from long-term sequelae such as vasomotor disturbances (associated with susceptibility to refreezing), and neuropathic and nociceptive pain, as well as damage to skeletal structures. There are still many uncertainties regarding the pathophysiology of these sequelae. It has been shown that the transient receptor potential channel (TRP) family plays a role in cold allodynia. Botulinum Toxin type A (BTX-A) injections have been reported to be beneficial in vasomotor and neuropathic disturbances secondary to frostbite. Epidural sympathetic block has been used for short-term treatment of frostbite induced chronic pain. Furthermore, amitriptyline, gabapentinoids, and duloxetine may have some benefits. Frostbite arthritis clinically resembles regular osteoarthritis. In children there is a risk of epiphyseal cartilage damage leading to bone deformities. Despite some promising therapeutic concepts, the scarcity of data on frostbite long-term sequelae in the literature indicates the need of more in-depth studies of this pathology in all its aspects.
Molecular Proteomics and Signalling of Human Platelets in Health and Disease
Authors: Huang Jingnan , Zhang Pengyu , Solari Fiorella A. , Sickmann Albert , Garcia Angel , Jurk Kerstin , Heemskerk Johan W. M.
Published: September 2021

Platelets are small anucleate blood cells that play vital roles in haemostasis and thrombosis, besides other physiological and pathophysiological processes. These roles are tightly regulated by a complex network of signalling pathways. Mass spectrometry-based proteomic techniques are contributing not only to the identification and quantification of new platelet proteins, but also reveal post-translational modifications of these molecules, such as acetylation, glycosylation and phosphorylation. Moreover, target proteomic analysis of platelets can provide molecular biomarkers for genetic aberrations with established or non-established links to platelet dysfunctions. In this report, we review 67 reports regarding platelet proteomic analysis and signalling on a molecular base. Collectively, these provide detailed insight into the: (i) technical developments and limitations of the assessment of platelet (sub)proteomes; (ii) molecular protein changes upon ageing of platelets; (iii) complexity of platelet signalling pathways and functions in response to collagen, rhodocytin, thrombin, thromboxane A2 and ADP; (iv) proteomic effects of endothelial-derived mediators such as prostacyclin and the anti-platelet drug aspirin; and (v) molecular protein changes in platelets from patients with congenital disorders or cardiovascular disease. However, sample sizes are still low and the roles of differentially expressed proteins are often unknown. Based on the practical and technical possibilities and limitations, we provide a perspective for further improvements of the platelet proteomic field.
3-Arylaziridine-2-carboxylic Acid Derivatives and (3-Arylaziridin-2-yl)ketones: The Aziridination Approaches
Authors: Strumfs Boriss , Uljanovs Romans , Velikijs Kirils , Trapencieris Peteris , Strumfa Ilze
Published: September 2021

Aziridination reactions represent a powerful tool in aziridine synthesis. Significant progress has been achieved in this field in the last decades, whereas highly functionalized aziridines including 3-arylated aziridine-2-carbonyl compounds play an important role in both medical and synthetic chemistry. For the reasons listed, in the current review we have focused on the ways to obtain 3-arylated aziridines and on the recent advances (mainly since the year 2000) in the methodology of the synthesis of these compounds via aziridination.
Alternative Splicing Regulation of Low-Frequency Genetic Variants in Exon 2 of TREM2 in Alzheimer’s Disease by Splicing-Based Aggregation
Authors: Han Seonggyun , Na Yirang , Koh Insong , Nho Kwangsik , Lee Younghee
Published: September 2021

TREM2 is among the most well-known Alzheimer’s disease (AD) risk genes; however, the functional roles of its AD-associated variants remain to be elucidated, and most known risk alleles are low-frequency variants whose investigation is challenging. Here, we utilized a splicing-guided aggregation method in which multiple low-frequency TREM2 variants were bundled together to investigate the functional impact of those variants on alternative splicing in AD. We analyzed whole genome sequencing (WGS) and RNA-seq data generated from cognitively normal elderly controls (CN) and AD patients in two independent cohorts, representing three regions in the frontal lobe of the human brain: the dorsolateral prefrontal cortex (CN = 213 and AD = 376), frontal pole (CN = 72 and AD = 175), and inferior frontal (CN = 63 and AD = 157). We observed an exon skipping event in the second exon of TREM2, with that exon tending to be more frequently skipped (p = 0.0012) in individuals having at least one low-frequency variant that caused loss-of-function for a splicing regulatory element. In addition, genes differentially expressed between AD patients with high vs. low skipping of the second exon (i.e., loss of a TREM2 functional domain) were significantly enriched in immune-related pathways. Our splicing-guided aggregation method thus provides new insight into the regulation of alternative splicing of the second exon of TREM2 by low-frequency variants and could be a useful tool for further exploring the potential molecular mechanisms of multiple, disease-associated, low-frequency variants.
Telomere Attrition and Clonal Hematopoiesis of Indeterminate Potential in Cardiovascular Disease
Authors: Huang Yi-Chun , Wang Chao-Yung
Published: September 2021

Clinical evidence suggests that conventional cardiovascular disease (CVD) risk factors cannot explain all CVD incidences. Recent studies have shown that telomere attrition, clonal hematopoiesis of indeterminate potential (CHIP), and atherosclerosis (telomere–CHIP–atherosclerosis, TCA) evolve to play a crucial role in CVD. Telomere dynamics and telomerase have an important relationship with age-related CVD. Telomere attrition is associated with CHIP. CHIP is commonly observed in elderly patients. It is characterized by an increase in blood cell clones with somatic mutations, resulting in an increased risk of hematological cancer and atherosclerotic CVD. The most common gene mutations are DNA methyltransferase 3 alpha (DNMT3A), Tet methylcytosine dioxygenase 2 (TET2), and additional sex combs-like 1 (ASXL1). Telomeres, CHIP, and atherosclerosis increase chronic inflammation and proinflammatory cytokine expression. Currently, their epidemiology and detailed mechanisms related to the TCA axis remain incompletely understood. In this article, we reviewed recent research results regarding the development of telomeres and CHIP and their relationship with atherosclerotic CVD.
Genome-Wide Mapping of Cytosine Methylation Revealed Dynamic DNA Methylation Patterns Associated with Sporophyte Development of Saccharina japonica
Authors: Yang Xiaoqi , Wang Xiuliang , Yao Jianting , Duan Delin
Published: September 2021

Cytosine methylation plays vital roles in regulating gene expression and plant development. However, the function of DNA methylation in the development of macroalgae remains unclear. Through the genome-wide bisulfite sequencing of cytosine methylation in holdfast, stipe and blade, we obtained the complete 5-mC methylation landscape of Saccharina japonica sporophyte. Our results revealed that the total DNA methylation level of sporophyte was less than 0.9%, and the content of CHH contexts was dominant. Moreover, the distribution of CHH methylation within the genes exhibited exon-enriched characteristics. Profiling of DNA methylation in three parts revealed the diverse methylation pattern of sporophyte development. These pivotal DMRs were involved in cell motility, cell cycle and cell wall/membrane biogenesis. In comparison with stipe and blade, hypermethylation of mannuronate C5-epimerase in holdfast decreased the transcript abundance, which affected the synthesis of alginate, the key component of cell walls. Additionally, 5-mC modification participated in the regulation of blade and holdfast development by the glutamate content respectively via glutamine synthetase and amidophosphoribosyl transferase, which may act as the epigenetic regulation signal. Overall, our study revealed the global methylation characteristics of the well-defined holdfast, stipe and blade, and provided evidence for epigenetic regulation of sporophyte development in brown macroalgae.
The Kynurenine Pathway—New Linkage between Innate and Adaptive Immunity in Autoimmune Endocrinopathies
Authors: Krupa Anna , Kowalska Irina
Published: September 2021

The kynurenine pathway (KP) is highly regulated in the immune system, where it promotes immunosuppression in response to infection or inflammation. Indoleamine 2,3-dioxygenase 1 (IDO1), the main enzyme of KP, has a broad spectrum of activity on immune cells regulation, controlling the balance between stimulation and suppression of the immune system at sites of local inflammation, relevant to a wide range of autoimmune and inflammatory diseases. Various autoimmune diseases, among them endocrinopathies, have been identified to date, but despite significant progress in their diagnosis and treatment, they are still associated with significant complications, morbidity, and mortality. The precise cellular and molecular mechanisms leading to the onset and development of autoimmune disease remain poorly clarified so far. In breaking of tolerance, the cells of the innate immunity provide a decisive microenvironment that regulates immune cells’ differentiation, leading to activation of adaptive immunity. The current review provided a comprehensive presentation of the known role of IDO1 and KP activation in the regulation of the innate and adaptive arms of the immune system. Significant attention has been paid to the immunoregulatory role of IDO1 in the most prevalent, organ-specific autoimmune endocrinopathies—type 1 diabetes mellitus (T1DM) and autoimmune thyroiditis.
Immunomodulatory Properties of BRAF and MEK Inhibitors Used for Melanoma Therapy—Paradoxical ERK Activation and Beyond
Authors: Jung Thomas , Haist Maximilian , Kuske Michael , Grabbe Stephan , Bros Matthias
Published: September 2021

The advent of mitogen-activated protein kinase (MAPK) inhibitors that directly inhibit tumor growth and of immune checkpoint inhibitors (ICI) that boost effector T cell responses have strongly improved the treatment of metastatic melanoma. In about half of all melanoma patients, tumor growth is driven by gain-of-function mutations of BRAF (v-rat fibrosarcoma (Raf) murine sarcoma viral oncogene homolog B), which results in constitutive ERK activation. Patients with a BRAF mutation are regularly treated with a combination of BRAF and MEK (MAPK/ERK kinase) inhibitors. Next to the antiproliferative effects of BRAF/MEKi, accumulating preclinical evidence suggests that BRAF/MEKi exert immunomodulatory functions such as paradoxical ERK activation as well as additional effects in non-tumor cells. In this review, we present the current knowledge on the immunomodulatory functions of BRAF/MEKi as well as the non-intended effects of ICI and discuss the potential synergistic effects of ICI and MAPK inhibitors in melanoma treatment.
Probabilistic Critical Controllability Analysis of Protein Interaction Networks Integrating Normal Brain Ageing Gene Expression Profiles
Authors: Yamaguchi Eimi , Akutsu Tatsuya , Nacher Jose C.
Published: September 2021

Recently, network controllability studies have proposed several frameworks for the control of large complex biological networks using a small number of life molecules. However, age-related changes in the brain have not been investigated from a controllability perspective. In this study, we compiled the gene expression profiles of four normal brain regions from individuals aged 20–99 years and generated dynamic probabilistic protein networks across their lifespan. We developed a new algorithm that efficiently identified critical proteins in probabilistic complex networks, in the context of a minimum dominating set controllability model. The results showed that the identified critical proteins were significantly enriched with well-known ageing genes collected from the GenAge database. In particular, the enrichment observed in replicative and premature senescence biological processes with critical proteins for male samples in the hippocampal region led to the identification of possible new ageing gene candidates.
Treatment with Edoxaban Attenuates Acute Stroke Severity in Mice by Reducing Blood–Brain Barrier Damage and Inflammation
Authors: Bieber Michael , Foerster Kathrin I. , Haefeli Walter E. , Pham Mirko , Schuhmann Michael K. , Kraft Peter
Published: September 2021

Patients with atrial fibrillation and previous ischemic stroke (IS) are at increased risk of cerebrovascular events despite anticoagulation. In these patients, treatment with non-vitamin K oral anticoagulants (NOAC) such as edoxaban reduced the probability and severity of further IS without increasing the risk of major bleeding. However, the detailed protective mechanism of edoxaban has not yet been investigated in a model of ischemia/reperfusion injury. Therefore, in the current study we aimed to assess in a clinically relevant setting whether treatment with edoxaban attenuates stroke severity, and whether edoxaban has an impact on the local cerebral inflammatory response and blood–brain barrier (BBB) function after experimental IS in mice. Focal cerebral ischemia was induced by transient middle cerebral artery occlusion in male mice receiving edoxaban, phenprocoumon or vehicle. Infarct volumes, functional outcome and the occurrence of intracerebral hemorrhage were assessed. BBB damage and the extent of local inflammatory response were determined. Treatment with edoxaban significantly reduced infarct volumes and improved neurological outcome and BBB function on day 1 and attenuated brain tissue inflammation. In summary, our study provides evidence that edoxaban might exert its protective effect in human IS by modulating different key steps of IS pathophysiology, but further studies are warranted.
A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine
Authors: Romano Eugenia , Netti Paolo Antonio , Torino Enza
Published: September 2021

In recent decades, endogenous nanocarrier-exosomes have received considerable scientific interest as drug delivery systems. The unique proteo-lipid architecture allows the crossing of various natural barriers and protects exosomes cargo from degradation in the bloodstream. However, the presence of this bilayer membrane as well as their endogenous content make loading of exogenous molecules challenging. In the present work, we will investigate how to promote the manipulation of vesicles curvature by a high-pressure microfluidic system as a ground-breaking method for exosomes encapsulation. Exosomes isolated from Uppsala 87 Malignant Glioma (U87-MG) cell culture media were characterized before and after the treatment with high-pressure homogenization. Once their structural and biological stability were validated, we applied this novel method for the encapsulation in the lipidic exosomal bilayer of the chemotherapeutic Irinotecan HCl Trihydrate-CPT 11. Finally, we performed in vitro preliminary test to validate the nanobiointeraction of exosomes, uptake mechanisms, and cytotoxic effect in cell culture model.
Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury
Authors: Su Vincent Yi-Fong , Chen Wei-Chih , Yu Wen-Kuang , Wu Huai-Hsuan , Chen Hao , Yang Kuang-Yao
Published: September 2021

The role of nintedanib, a multiple tyrosine kinase inhibitor, in the treatment of sepsis-induced acute lung injury (ALI) remains unclear. Lipopolysaccharide (LPS), also known as endotoxin, has been used to induce ALI. The goal of this study was to assess the effect of nintedanib in attenuating the histopathological changes of LPS-induced ALI. Nintedanib was administered via oral gavage to male C57BL/6 mice 24 h and 10 min before intratracheal endotoxin instillation. Lung histopathological characteristics, adhesion molecule expression, and the regulatory signaling pathways of neutrophil chemotaxis were analyzed after 24 h. We found that nintedanib significantly reduced histopathological changes and neutrophil recruitment in LPS-induced ALI. The number of neutrophils in bronchoalveolar lavage fluid (BALF) was reduced in nintedanib-treated relative to untreated mice with ALI. Nintedanib mediated the downregulation of the chemotactic response to LPS by reducing the expression of adhesion molecules and the phosphorylated p38:total p38 mitogen-activated protein kinase (MAPK) ratio in the lungs of mice with ALI. Nintedanib also reduced the expression of lymphocyte antigen 6 complex locus G6D (Ly6G) and very late antigen 4 (VLA-4) in BALF neutrophils and mediated the downregulation of chemokine (C-X-C motif) receptor 2 (CXCR2) and upregulation of G protein-coupled receptor kinase 2 (GRK2) activity in peripheral blood neutrophils in mice with LPS-induced ALI. Nintedanib improved the histopathological changes of LPS-induced ALI by reducing neutrophil chemotaxis. These effects were mediated by the inhibition of adhesion molecules via the activation of GRK2 and the inhibition of p38 MAPK and CXCR2.
Ferroptosis Mechanisms Involved in Hippocampal-Related Diseases
Authors: Wang Xintong , Wang Zixu , Cao Jing , Dong Yulan , Chen Yaoxing
Published: September 2021

Ferroptosis is a newly recognized type of cell death that is different from traditional forms of cell death, such as apoptosis, autophagy, and necrosis. It is caused by the accumulation of intracellular iron, promoting lipid peroxidation and leading to cell death. Iron is essential as a redox metal in several physiological functions. The brain is one of the organs known to be affected by iron homeostatic balance disruption. An increased concentration of iron in the central nervous system has been associated with oxidative stress, lipid peroxidation of proteins, and cell death. The hippocampus is an important brain region for learning, memory, and emotional responses, and is also a sensitive part of the brain to the dysfunctional homeostasis of transition metals. Damage of hippocampal structure and function are intimately involved in the pathogenic mechanisms underlying neurodegenerative diseases. Currently, ferroptosis is playing an increasingly important role in treatment areas of central nervous system diseases. Thus, we provide an overview of ferroptosis regulatory mechanisms, such as lipid metabolism, glutathione metabolism, and iron metabolism in this review. We also highlight the role of ferroptosis in hippocampal-related diseases and investigate a theoretical basis for further research on the role of ferroptosis in nervous system disease treatment.
Analysis of the Peripapillary and Macular Regions Using OCT Angiography in Patients with Schizophrenia and Bipolar Disorder
Authors: Koman-Wierdak Edyta , Róg Joanna , Brzozowska Agnieszka , Toro Mario Damiano , Bonfiglio Vincenza , Załuska-Ogryzek Katarzyna , Karakuła-Juchnowicz Hanna , Rejdak Robert , Nowomiejska Katarzyna
Published: September 2021

Purpose: To measure RNFL and vasculature around the optic disc and in the macula in patients with schizophrenia (SZ) and bipolar disorder (BD) using optical coherence tomography angiography (OCTA). Methods: 24 eyes of patients with SZ and 16 eyes of patients with BD as well as 30 eyes of healthy subjects were examined with OCTA. The radiant peripapillary capillary (RPC) density and RNFL thickness were measured in the peripapillary area. Moreover, macular thickness and vessel density were measured in both superficial and deep layers. Results: Significantly decreased values of vessel density in the macular deep vascular complex were found in the eyes of patients with SZ, compared to BD and the control group. The macular thickness in the whole vascular complex and in the fovea was significantly lower in SZ and BD group than in the control group. The radiant peripapillary vascular density and RNFL thickness were similar across groups. Conclusions: The retinal microvascular dysfunction occurs in the macula in patients with SZ and BD, but not around optic disc. OCTA can become an essential additional diagnostic tool in detection of psychiatric disorders.
Development of an Augmented Reality Concept for Icebreaker Assistance and Convoy Operations
Authors: Frydenberg Synne , Aylward Katie , Nordby Kjetil , Eikenes Jon Olav H.
Published: September 2021

A vessel convoy is a complex and high-risk operation completed during icebreaking operations in the Arctic. Icebreaker navigators need to continuously communicate with their crew while monitoring information such as speed, heading, and distance between vessels in the convoy. This paper presents an augmented reality user interface concept, which aims to support navigators by improving oversight and safety during convoy operations. The concept demonstrates how augmented reality can help to realize a situated user interface that adapts to user’s physical and operational contexts. The concept was developed through a human-centered design process and tested through a virtual reality simulator in a usability study involving seven mariners. The results suggest that augmented reality has the potential to improve the safety of convoy operations by integrating distributed information with heads-up access to operation-critical information. However, the user interface concept is still novel, and further work is needed to develop the concept and safely integrate augmented reality into maritime operations.
Offshore Structural Reliability Assessment by Probabilistic Procedures—A Review
Authors: Syed Ahmad Sayyid Zainal Abidin , Abu Husain Mohd Khairi , Mohd Zaki Noor Irza , Mukhlas Nurul Azizah , Mat Soom Ezanizam , Azman Nurul Uyun , Najafian Gholamhossein
Published: September 2021

Offshore installations must be built to resist fatigue as well as extreme forces caused by severe environmental conditions. The structural reliability analysis is the popular practise to assess a variety of natural waves determined by the long-term probability distribution of wave heights and corresponding periods on the site. In truth, however, these structures are subjected to arbitrary wave-induced forces in the open ocean. Hence, it is much more reasonable to account for the changed loading characteristics by determining the probabilistic characteristics of the random loads and outcomes responses. The key challenges are uncertainties and the non-linearity of Morison’s drag element, which results in non-Gaussian loading and response distributions. This study would analyze advances achieved to date in a comprehensive probabilistic review of offshore fixed jacket-type platforms.
Double-Loop Sliding Mode Controller with An Ocean Current Observer for the Trajectory Tracking of ROV
Authors: Mu Weilei , Wang Yuxue , Sun Hailiang , Liu Guijie
Published: September 2021

To solve the trajectory tracking problem of insufficient response and the large tracking error of remotely operated vehicles (ROVs) under the interference of large ocean currents, this paper proposes a double-loop sliding mode controller with an ocean current observer. The designed controller consisted of an outer-loop controller (the position controller) and an inner-loop controller (the velocity controller): the outer controller was designed by the position error, and a reference velocity was created for the inner loop to achieve accurate positioning and attitude tracking. The reference control input was treated as a new target to design the inner-loop controller, enabling the ROV to achieve accurate reference velocity tracking. Based on the theoretical idea of active disturbance rejection control, a kinematic equation-based ocean current observer was designed to estimate and compensate for large unknown currents to ensure accurate trajectory tracking performance under large currents. The simulation results proved that the double-loop sliding-mode control scheme with an ocean current observer always showed good tracking performance, demonstrating the excellent control performance and high robustness of the scheme. Compared with the high-complexity control schemes such as neural network-based PID control or fuzzy sliding mode control, it effectively improves the robustness to ocean current disturbances without increasing the computational effort excessively, and is more practical in ROV systems with limited computational power.
Method for Prediction of Extreme Wave Loads Based on Ship Operability Analysis Using Hindcast Wave Database
Authors: Petranović Tamara , Mikulić Antonio , Katalinić Marko , Ćorak Maro , Parunov Joško
Published: September 2021

The method for the prediction of extreme vertical wave bending moments on a passenger ship based on the hindcast database along the shipping route is presented. Operability analysis is performed to identify sea states when the ship is not able to normally operate and which are likely to be avoided. Closed-form expressions are used for the calculation of transfer functions of ship motions and loads. Multiple operability criteria are used and compared to the corresponding limiting values. The most probable extreme wave bending moments for the short-term sea states at discrete locations along the shipping route are calculated, and annual maximum extreme values are determined. Gumbel probability distribution is then fitted to the annual extreme values, and wave bending moments corresponding to a return period of 20 years are determined for discrete locations. The system reliability approach is used to calculate combined extreme vertical wave bending moment along the shipping route. The method is employed on the example of a passenger ship sailing across the Adriatic Sea (Split, Croatia, to Ancona, Italy). The contribution of the study is the method for the extreme values of wave loads using the hindcast wave database and accounting for ship operational restrictions.
ITS2 Sequencing and Targeted Meta-Proteomics of Infant Gut Mycobiome Reveal the Functional Role of Rhodotorula sp. during Atopic Dermatitis Manifestation
Authors: Mok Kevin , Suratanon Narissara , Roytrakul Sittiruk , Charoenlappanit Sawanya , Patumcharoenpol Preecha , Chatchatee Pantipa , Vongsangnak Wanwipa , Nakphaichit Massalin
Published: September 2021

Association between the gut mycobiome and atopic dermatitis was investigated in 9–12-month-old infants using metagenomics. Two groups of atopic dermatitis infants were classified according to their symptom development as outgrown (recovered) and persisted (still undergoing). The evenness and diversity of the mycobiome in the persisted group were higher than in the healthy and outgrown groups. Dysbiosis of the microbiome in the persisted group was observed by a reduction in the Ascomycota/Basidiomycota ratio. Five fungi were selected as markers from each sample group. In the persisted group, Rhodotorula sp. abundance increased significantly, while Wickerhamomyces sp. and Kodamaea sp. abundance increased in the healthy group, and Acremonium sp. and Rhizopus sp. abundance increased considerably in the outgrown group. Metaproteomic analysis revealed that the persisted group had a high abundance of fungal proteins, particularly those from Rhodotorula sp. Unique proteins such as RAN-binding protein 1 and glycerol kinase from Rhodotorula sp. were hypothesized to be related to atopic dermatitis manifestation in infants.
Effect of a Wood-Based Carrier of Trichoderma atroviride SC1 on the Microorganisms of the Soil
Authors: Chammem Hamza , Antonielli Livio , Nesler Andrea , Pindo Massimo , Pertot Ilaria
Published: September 2021

Wood pellets can sustain the growth of Trichoderma spp. in soil; however, little is known about their side effects on the microbiota. The aims of this study were to evaluate the effect of wood pellets on the growth of Trichoderma spp. in bulk soil and on the soil microbial population’s composition and diversity. Trichoderma atroviride SC1 coated wood pellets and non-coated pellets were applied at the level of 10 g∙kg−1 of soil and at the final concentration of 5 × 103 conidia∙g−1 of soil and compared to a conidial suspension applied at the same concentration without the wood carrier. Untreated bulk soil served as a control. The non-coated wood pellets increased the total Trichoderma spp. population throughout the experiment (estimated as colony-forming unit g−1 of soil), while wood pellets coated with T. atroviride SC1 did not. The wood carrier increased the richness, and temporarily decreased the diversity, of the bacterial population, with Massilia being the most abundant bacterial genus, while it decreased both the richness and diversity of the fungal community. Wood pellets selectively increased fungal species having biocontrol potential, such as Mortierella, Cladorrhinum, and Stachybotrys, which confirms the suitability of such carriers of Trichoderma spp. for soil application.
Response of NDVI of Natural Vegetation to Climate Changes and Drought in China
Authors: Wang Huaijun , Li Zhi , Cao Lei , Feng Ru , Pan Yingping
Published: September 2021

Temporal and spatial changes in vegetation and their influencing factors are of great significance for the assessment of climate change and sustainable development of ecosystems. This study applied the Asymmetric Gaussians (AG) fitting method, Mann-Kendall test, and correlation analysis to the Global Inventory Monitoring and Modeling System (GIMMS) third-generation Normalized Difference Vegetation Index and gridded climate and drought data for 1982–2015. The temporal and spatial changes to NDVI for natural grassland and forest during the growing season were analyzed. Relationships among NDVI, climate change, and droughts were also analyzed to reveal the influence of vegetation change. The results showed that: (1) Land use/cover change (LUCC) in China was mainly represented by increases in agricultural land (Agrl) and urban and rural land (Uril), and decreases in unutilized land (Bald), grassland, forest, and permanent glacier and snow (Snga). The increase in agricultural land was mainly distributed in the western northwest arid area (WNW) and northern North China (NNC), whereas regions with severe human activities such as southern South China (SNC), western South China (WSC), and eastern South China (ESC) showed significant decreases in agricultural land due to conversion to urban and rural land. (2) The start of the growing season (SOS) was advanced in WNW, SNC, WSC, and ESC, and the end of growing season (EOS) was delayed in WNW, NNC, and SNC. The growing season length (GSL) of natural vegetation in China has been extended by eight days over the last 34 years. However, the phenology of the Qinghai-Tibet Plateau (TP) was opposite to that of the other regions and the GSL showed an insignificant decreasing trend. (3) The NDVI increased significantly, particularly in the SNC, WSC, ESC, and the grassland of the WNW. Precipitation was found to mainly control the growth of vegetation in the arid and semi-arid regions of northwest China (WNW and ENW), and precipitation had a much greater impact on grassland than on forests. Temperature had an impact on the growth of vegetation throughout China, particularly in SNC, ESC, and WSC. (4) The Standardized Precipitation Evapotranspiration Index (SPEI) showed a downward trend, indicating an aridification trend in China, particularly in ENW, NNC, and WNW. Similar to precipitation, the main areas affected by drought were WNW and ENW and grassland was found to be more sensitive to drought than forest. The results of this study are of great significance for predicting the response of ecosystem productivity to climate change under future climate change scenarios.
Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure
Authors: Blume Gustavo Gavazzoni , Machado-Junior Paulo André Bispo , Simeoni Rossana Baggio , Bertinato Giovana Paludo , Tonial Murilo Sgarbossa , Nagashima Seigo , Pinho Ricardo Aurino , de Noronha Lucia , Olandoski Marcia , de Carvalho Katherine Athayde Teixeira , Francisco Julio Cesar , Guarita-Souza Luiz Cesar
Published: September 2021

Myocardial infarction (MI) remains the leading cause of cardiovascular death worldwide and a major cause of heart failure. Recent studies have suggested that cell-based therapies with bone marrow stem cells (BMSC) and human amniotic membrane (hAM) would recover the ventricular function after MI; however, the mechanisms underlying these effects are still controversial. Herein, we aimed to compare the effects of BMSC and hAM in a rat model of heart failure. MI was induced through coronary occlusion, and animals with an ejection fraction (EF) < 50% were included and randomized into three groups: control, BMSC, and hAM. The BMSC and hAM groups were implanted on the anterior ventricular wall seven days after MI, and a new echocardiographic analysis was performed on the 30th day, followed by euthanasia. The echocardiographic results after 30 days showed significant improvements on EF and left-ventricular end-sistolic and end-diastolic volumes in both BMSC and hAM groups, without significant benefits in the control group. New blood vessels, desmine-positive cells and connexin-43 expression were also elevated in both BMSC and hAM groups. These results suggest a recovery of global cardiac function with the therapeutic use of both BMSC and hAM, associated with angiogenesis and cardiomyocyte regeneration after 30 days.
Improving the Tribological Properties of Technical Polymers with Metal Sulphide Compounds
Authors: Hausberger Andreas , Stiller Tanja , Kappl Clemens , Hensgen Lars , Grün Florian
Published: September 2021

Technical thermoplastic materials (e.g., PEEK, PPA and POM) are widely used for tribological applications combined with different filler systems (e.g., glass- or carbon fibres) because of their excellent mechanical properties. The friction and wear behaviour of thermoplastics can be specifically improved by solid lubrication systems such as graphite, PTFE and MoS2. Besides these systems, others such as WoS2 and MnS are becoming scientifically interesting. This work investigates the influence of different solid lubricants—alternative metal sulphides and polymer-based—in combination with different glass fibre contents on the tribological behaviour of unfilled PEEK and glass fibre-filled PPA. For this purpose, compounds were produced and injection-moulded into tribological test specimens that were subsequently tested. It is particularly evident for both matrix materials that the solid lubricant SLS 22 shows a 25% wear rate reduction when compared to MoS2 and, in addition, the proportion of fibre content in PPA shows an additional wear rate reduction by a factor of 10. The friction level could be kept at a similar level compared to the usually utilised solid lubricants. The investigations showed the potential use of metal sulphide filler systems in high-performance thermoplastic with enhanced tribological properties as alternatives to the well-established solid lubricants.
Green Copolymers Based on Poly(Lactic Acid)—Short Review
Authors: Stefaniak Konrad , Masek Anna
Published: September 2021

Polylactic acid (PLA) is a biodegradable and biocompatible polymer that can be applied in the field of packaging and medicine. Its starting substrate is lactic acid and, on this account, PLA can also be considered an ecological material produced from renewable resources. Apart from several advantages, polylactic acid has drawbacks such as brittleness and relatively high glass transition and melting temperatures. However, copolymerization of PLA with other polymers improves PLA features, and a desirable material marked by preferable physical properties can be obtained. Presenting a detailed overview of the accounts on the PLA copolymerization accomplishments is the innovation of this paper. Scientific findings, examples of copolymers (including branched, star, grafted or block macromolecules), and its applications are discussed. As PLA copolymers can be potentially used in pharmaceutical and biomedical areas, the attention of this article is also placed on the advances present in this field of study. Moreover, the subject of PLA synthesis is described. Three methods are given: azeotropic dehydrative condensation, direct poly-condensation, and ring-opening polymerization (ROP), along with its mechanisms. The applied catalyst also has an impact on the end product and should be adequately selected depending on the intended use of the synthesized PLA. Different ways of using stannous octoate (Sn(Oct)2) and examples of the other inorganic and organic catalysts used in PLA synthesis are presented.
Process Window for Highly Efficient Laser-Based Powder Bed Fusion of AlSi10Mg with Reduced Pore Formation
Authors: Leis Artur , Weber Rudolf , Graf Thomas
Published: September 2021

The process window for highly efficient laser-based powder bed fusion (LPBF), ensuring the production of parts with low porosity, was determined by analyzing cross-sections of samples that were generated with laser powers varying between 10.8 W and 1754 W, laser beam diameters varying between 35 μm and 200 μm, and velocities of the moving laser beam ranging between 0.7 m/s and 1.3 m/s. With these parameters, the process alters between different modes that are referred to as simple heating, heat conduction melting (HCM), key-bowl melting (KBM), and deep-penetration melting (DPM). It was found that the optimum process window for a highly efficient LPBF process, generating AlSi10Mg parts with low porosity, is determined by the ratio PL/db of the incident laser power PL and the beam diameter db of the beam on the surface of the bead, and ranges between PL/db = 2000 W/mm and PL/db = 5200 W/mm, showing process efficiencies of about 7–8%. This optimum process window is centered around the range PL/db = 3000–3500 W/mm, in which the process is characterized by KBM, which is an intermediate process mode between HCM and DPM. Processes with PL/db < 2000 W/mm partially failed, and lead to balling and a lack of fusion, whereas processes with PL/db > 5200 W/mm showed a process efficiency below 5% and pore ratios exceeding 10%.
Full-Field Temperature Measurement of Stainless Steel Specimens Subjected to Uniaxial Tensile Loading at Various Strain Rates
Authors: Żaba Krzysztof , Trzepieciński Tomasz , Rusz Stanislav , Puchlerska Sandra , Balcerzak Maciej
Published: September 2021

This article presents a study on the effect of strain rate, specimen orientation, and plastic strain on the value and distribution of the temperature of dog-bone 1 mm-thick specimens during their deformation in uniaxial tensile tests. Full-field image correlation and infrared thermography techniques were used. A titanium-stabilised austenitic 321 stainless steel was used as test materials. The dog-bone specimens used for uniaxial tensile tests were cut along the sheet metal rolling direction and three strain rates were considered: 4 × 10−3 s−1, 8 × 10−3 s−1 and 16 × 10−3 s−1. It was found that increasing the strain rate resulted in the intensification of heat generation. High-quality regression models (Ra > 0.9) developed for the austenitic 321 steel revealed that sample orientation does not play a significant role in the heat generation when the sample is plastically deformed. It was found that at the moment of formation of a necking at the highest strain rate, the maximum sample temperature increased more than four times compared to the initial temperature. A synergistic effect of the strain hardening exponent and yield stress revealed that heat is generated more rapidly towards small values of strain hardening exponent and yield stress.
Formation of Solid Solutions and Physicochemical Properties of the High-Entropy Ln1−xSrx(Co,Cr,Fe,Mn,Ni)O3−δ (Ln = La, Pr, Nd, Sm or Gd) Perovskites
Authors: Dąbrowa Juliusz , Zielińska Klaudia , Stępień Anna , Zajusz Marek , Nowakowska Margarita , Moździerz Maciej , Berent Katarzyna , Szymczak Maria , Świerczek Konrad
Published: September 2021

Phase composition, crystal structure, and selected physicochemical properties of the high entropy Ln(Co,Cr,Fe,Mn,Ni)O3−δ (Ln = La, Pr, Gd, Nd, Sm) perovskites, as well as the possibility of Sr doping in Ln1−xSrx(Co,Cr,Fe,Mn,Ni)O3−δ series, are reported is this work. With the use of the Pechini method, all undoped compositions are successfully synthesized. The samples exhibit distorted, orthorhombic or rhombohedral crystal structure, and a linear correlation is observed between the ionic radius of Ln and the value of the quasi-cubic perovskite lattice constant. The oxides show moderate thermal expansion, with a lack of visible contribution from the chemical expansion effect. Temperature-dependent values of the total electrical conductivity are reported, and the observed behavior appears distinctive from that of non-high entropy transition metal-based perovskites, beyond the expectations based on the rule-of-mixtures. In terms of formation of solid solutions in Sr-doped Ln1−xSrx(Co,Cr,Fe,Mn,Ni)O3−δ materials, the results indicate a strong influence of the Ln radius, and while for La-based series the Sr solubility limit is at the level of xmax = 0.3, for the smaller Pr it is equal to just 0.1. In the case of Nd-, Sm- and Gd-based materials, even for the xSr = 0.1, the formation of secondary phases is observed on the SEM + EDS images.
Insights into the Intrinsic Factors Affecting the NIR Reflectance Based on Rylene Diimide Molecules
Authors: Zeng Weili , Song Yujie , Zhang Jianning , Chen Hong , Liu Ming , Chen Wangqiao
Published: September 2021

A clear understanding of the relationships between molecular structure and NIR reflectance (700–2500 nm) behavior is important and highly desirable for developing appropriate NIR-reflective materials to combat NIR heat radiation from sunlight. In this research, three groups of imide-based compounds have been adopted to investigate the influence of the intrinsic molecular structures on the NIR-reflective properties. It is found out that for the compounds with alkyl groups, the NIR reflectance will increase as the degree of the conjugated backbone increases, especially for the reflectance from 1750 nm to 2500 nm. In addition, despite that the alkyl or amine groups deteriorate the NIR reflectance, the NIR reflectance varies within a certain interval and the isomers with branched alkyl groups show identical or smaller NIR reflectance than those of isomers with linear alkyl groups. For different compounds, crystallinity seems to almost have no relationship with their NIR reflectance.
Simulation of Subrapid Solidification and Secondary Cooling for the Strip Casting of IF Steel
Authors: Wang Wanlin , Mao Song , Zhang Hualong , Lu Cheng , Lyu Peisheng
Published: September 2021

A combination of droplet solidification tester and confocal laser scanning microscope was used to simulate subrapid solidification and secondary cooling process pertinent to the strip casting. The IF steel droplet had a delamination structure and the bottom part went through sub-rapid solidification. During secondary cooling, γ/α transformation mechanism belonged to interface-controlled massive transformation and the ferrite grains grew quickly. With the increase of cooling rate, the γ/α transformation temperature decreased and the incubation period and phase transformation duration reduced. The hardness showed a slight increase due to fine-grain strengthening. With coiling temperature increasing from 600 °C to 800 °C, the grain size became larger, precipitates became coarse, and defects in grain were recovered. Consequently, the hardness decreased.
Subsampling and Aggregation: A Solution to the Scalability Problem in Distance-Based Prediction for Mixed-Type Data
Authors: Baíllo Amparo , Grané Aurea
Published: September 2021

The distance-based linear model (DB-LM) extends the classical linear regression to the framework of mixed-type predictors or when the only available information is a distance matrix between regressors (as it sometimes happens with big data). The main drawback of these DB methods is their computational cost, particularly due to the eigendecomposition of the Gram matrix. In this context, ensemble regression techniques provide a useful alternative to fitting the model to the whole sample. This work analyzes the performance of three subsampling and aggregation techniques in DB regression on two specific large, real datasets. We also analyze, via simulations, the performance of bagging and DB logistic regression in the classification problem with mixed-type features and large sample sizes.
Soft Frames in Soft Hilbert Spaces
Authors: Ferrer Osmin , Sierra Arley , Sanabria José
Published: September 2021

In this paper, we use soft linear operators to introduce the notion of discrete frames on soft Hilbert spaces, which extends the classical notion of frames on Hilbert spaces to the context of algebraic structures on soft sets. Among other results, we show that the frame operator associated to a soft discrete frame is bounded, self-adjoint, invertible and with a bounded inverse. Furthermore, we prove that every element in a soft Hilbert space satisfies the frame decomposition theorem. This theoretical framework is potentially applicable in signal processing because the frame coefficients serve to model the data packets to be transmitted in communication networks.