bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into the Phytochemistry of the Cuban Endemic Medicinal Plant Phyllanthus orbicularis: Fideloside, a Novel Bioactive 8-C-glycosyl 2,3-Dihydroflavonol
Authors: Francioso Antonio , Franke Katrin , Villani Claudio , Mosca Luciana , D’Erme Maria , Frischbutter Stefan , Brandt Wolfgang , Sanchez-Lamar Angel , Wessjohann Ludger
Published: August 2019

Phyllanthus orbicularis (Phyllanthaceae) is an endemic evergreen tropical plant of Cuba that grows in the western part of the island and is used in traditional medicine as an infusion. The aqueous extract of this plant presents a wide range of pharmacological activitiessuch as antimutagenic, antioxidant and antiviral effects. Given the many beneficial effects and the great interest in the development of new pharmacological products from natural sources, the aim of this work was to investigate the phytochemistry of this species and to elucidate the structure of the main bioactive principles. Besides the presence of several known polyphenols, the major constituent was hitherto not described. The chemical structure of this compound, here named Fideloside, was elucidated by means of HR-ESIMS/MSn, 1D/2D NMR, FT-IR, and ECD as (2R,3R)-(−)-3’,4′,5,7-tetrahydroxydihydroflavonol-8-C-β-D-glucopyranoside. The compound, as well as the plant aqueous preparations, showed promising bioactive properties, i.e., anti-inflammatory capacity in human explanted monocytes, corroborating future pharmacological use for this new natural C-glycosyl flavanonol.
Introduction to “Intramolecular Hydrogen Bonding 2018”
Authors: Sánchez Goar
Published: August 2019

Non-covalent interactions have attracted the scientific attention during last decades as observed by the numerous studies in the literature [...]
Interaction with Blood Proteins of a Ruthenium(II) Nitrofuryl Semicarbazone Complex: Effect on the Antitumoral Activity
Authors: Demoro Bruno , Bento-Oliveira Andreia , Marques Fernanda , Costa Pessoa João , Otero Lucía , Gambino Dinorah , F. M. de Almeida Rodrigo , Tomaz Ana Isabel
Published: August 2019

The steady rise in the cancer burden and grim statistics set a vital need for new therapeutic solutions. Given their high efficiency, metallodrugs are quite appealing in cancer chemotherapy. This work examined the anticancer activity of an anti-trypanosomal ruthenium-based compound bearing the 5-nitrofuryl pharmacophore, [RuII(dmso)2(5-nitro-2-furaldehyde semicarbazone)] (abbreviated as RuNTF; dmso is the dimethyl sulfoxide ligand). The cytotoxicity of RuNTF was evaluated in vitro against ovarian adenocarcinoma, hormone-dependent breast adenocarcinoma, prostate carcinoma (grade IV) and V79 lung fibroblasts human cells. The activity of RuNTF was similar to the benchmark metallodrug cisplatin for the breast line and inactive against the prostate line and lung fibroblasts. Given the known role of serum protein binding in drug bioavailability and the distribution via blood plasma, this study assessed the interaction of RuNTF with human serum albumin (HSA) by circular dichroism (CD) and fluorescence spectroscopy. The fluorescence emission quenching from the HSA-Trp214 residue and the lifetime data upon RuNTF binding evidenced the formation of a 1:1 {RuNTF-albumin} adduct with log Ksv = (4.58 ± 0.01) and log KB = (4.55 ± 0.01). This is supported by CD data with an induced CD broad band observed at ~450 nm even after short incubation times. Importantly, the binding to either HSA or human apo-transferrin is beneficial to the cytotoxicity of the complex towards human cancer cells by enhancing the cytotoxic activity of RuNTF.
Efficacy of a Bispecific Antibody Co-Targeting VEGFA and Ang-2 in Combination with Chemotherapy in a Chemoresistant Colorectal Carcinoma Xenograft Model
Authors: Mueller Thomas , Freystein Juana , Lucas Henrike , Schmoll Hans-Joachim
Published: August 2019

Vascular endothelial growth factor (VEGF) inhibition by the addition of bevacizumab to the chemotherapy regimen of metastatic colorectal cancer leads to an improved outcome. However, anti-angiogenic tumor therapy targeting a single factor may be limited by complementary mechanisms. Angiopoietin-2 (Ang-2, ANGPT2) is another important factor that cooperates with VEGF to drive tumor angiogenesis. It was shown that high Ang-2 levels are associated with a poor clinical outcome of colorectal cancer patients treated with bevacizumab-containing therapy. Therefore, combined inhibition of VEGF and Ang-2 was supposed to improve anti-angiogenic therapy. Here, we evaluated the efficacy of a bispecific antibody (CrossMab) co-targeting VEGF and Ang-2 in combination with chemotherapy in a chemoresistant colorectal carcinoma model. Antitumor activity was evaluated in athymic nude mice bearing subcutaneous DLD1 xenograft tumors and treated with anti-VEGF (B20), anti-Ang-2 (LC06) and anti-VEGF/Ang-2 (CrossMab) antibodies. Chemotherapy consisted of 5-FU and irinotecan. Resected tumors were analyzed immunohistochemically. First, an impact of targeting each single factor but also a clear advantage of co-targeting both factors could be demonstrated. Accordingly, tumor tissue showed strong staining for VEGF and Ang-2. Chemotherapy alone was less effective. Efficient tumor growth inhibition could be achieved by treatment with anti-VEGF/chemotherapy, single CrossMab and CrossMab/chemotherapy, which resulted in 3 out of 10, 6 out of 10 and 10 out of 10 complete responses, respectively, during seven weeks. Complete retarded tumors were characterized by massive intratumoral necrosis surrounded by layers of vital tumor cells and connective tissue with CD31-positive vessels at the periphery. In some cases, a distinct feature known as vessel co-option could be observed. In conclusion, the data from this model clearly support the strategy of co-targeting VEGF and Ang-2 and further demonstrate the beneficial impact of co-treatment with chemotherapy. The clear superiority of the CrossMab-containing regimen compared to clinical standard anti-VEGF/chemotherapy warrants further analyses in other models.
A Comprehensive Review of Non-Covalent Radiofluorination Approaches Using Aluminum [18F]fluoride: Will [18F]AlF Replace 68Ga for Metal Chelate Labeling?
Authors: Fersing Cyril , Bouhlel Ahlem , Cantelli Christophe , Garrigue Philippe , Lisowski Vincent , Guillet Benjamin
Published: August 2019

Due to its ideal physical properties, fluorine-18 turns out to be a key radionuclide for positron emission tomography (PET) imaging, for both preclinical and clinical applications. However, usual biomolecules radiofluorination procedures require the formation of covalent bonds with fluorinated prosthetic groups. This drawback makes radiofluorination impractical for routine radiolabeling, gallium-68 appearing to be much more convenient for the labeling of chelator-bearing PET probes. In response to this limitation, a recent expansion of the 18F chemical toolbox gave aluminum [18F]fluoride chemistry a real prominence since the late 2000s. This approach is based on the formation of an [18F][AlF]2+ cation, complexed with a 9-membered cyclic chelator such as NOTA, NODA or their analogs. Allowing a one-step radiofluorination in an aqueous medium, this technique combines fluorine-18 and non-covalent radiolabeling with the advantage of being very easy to implement. Since its first reports, [18F]AlF radiolabeling approach has been applied to a wide variety of potential PET imaging vectors, whether of peptidic, proteic, or small molecule structure. Most of these [18F]AlF-labeled tracers showed promising preclinical results and have reached the clinical evaluation stage for some of them. The aim of this report is to provide a comprehensive overview of [18F]AlF labeling applications through a description of the various [18F]AlF-labeled conjugates, from their radiosynthesis to their evaluation as PET imaging agents.
Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices
Authors: Lim Hosub , Jafry Ali Turab , Lee Jinkee
Published: August 2019

Paper-based microfluidic devices have advanced significantly in recent years as they are affordable, automated with capillary action, portable, and biodegradable diagnostic platforms for a variety of health, environmental, and food quality applications. In terms of commercialization, however, paper-based microfluidics still have to overcome significant challenges to become an authentic point-of-care testing format with the advanced capabilities of analyte purification, multiplex analysis, quantification, and detection with high sensitivity and selectivity. Moreover, fluid flow manipulation for multistep integration, which involves valving and flow velocity control, is also a critical parameter to achieve high-performance devices. Considering these limitations, the aim of this review is to (i) comprehensively analyze the fabrication techniques of microfluidic paper-based analytical devices, (ii) provide a theoretical background and various methods for fluid flow manipulation, and (iii) highlight the recent detection techniques developed for various applications, including their advantages and disadvantages.
The Necessity of Philosophy in the Exercise Sciences
Authors: Hickson Matthew
Published: August 2019

The pervasive and often uncritical acceptance of materialistic philosophical commitments within exercise science is deeply problematic. This commitment to materialism is wrong for several reasons. Among the most important are that it ushers in fallacious metaphysical assumptions regarding the nature of causation and the nature of human beings. These mistaken philosophical commitments are key because the belief that only matter is real severely impedes the exercise scientist’s ability to accurately understand or deal with human beings, whether as subjects of study or as data points to be interpreted. One example of materialist metaphysics is the assertion that all causation is physical- one lever moving another lever, one atom striking another atom, one brain state leading to another (Kretchmer, 2005). In such a world, human life is reduced to action and reaction, stimulus and response and as a result, the human being disappears. As such, a deterministic philosophy is detrimental to kinesiologists’ attempts to interpret and understand human behavior, for a materialistic philosophy, must ignore or explain away human motivation, human freedom and ultimately culture itself. In showing how mistaken these philosophic commitments are, I will focus on the sub-discipline of sport psychology for most examples, as that is the field of exercise science of which I am paradigmatically most familiar. It is also the field, when rightly understood that straddles the “two cultures” in kinesiology (i.e., the sciences and the humanities). In referencing the dangers of the materialistic conception of human beings for sport psychology, I will propose, that the materialist’s account of the natural world, causation and human beings stems from the unjustified and unnecessary rejection by the founders of modern science of the Aristotelian picture of the world (Feser, 2012). One reason that this mechanistic point of view, concerning human reality has gained ground in kinesiology is as a result of a previous philosophic commitment to quantification. As philosopher Doug Anderson (2002) has pointed out, many kinesiologists believe that shifting the discipline in the direction of mathematics and science would result in enhanced academic credibility. Moreover, given the dominance of the scientific narrative in our culture it makes it very difficult for us not to conform to it. That is, as Twietmeyer (2015) argued, kinesiologists do not just reject non-materialistic philosophic conceptions of the field, we are oblivious to their possibility. Therefore, I will propose two things; first, Aristotelian philosophy is a viable alternative to materialistic accounts of nature and causation and second, that Aristotle’s holistic anthropology is an important way to wake kinesiologists from their self-imposed philosophic slumber.
An Investigation on the Effect of the Total Efficiency of Water and Air Used Together as a Working Fluid in the Photovoltaic Thermal Systems
Authors: Atmaca Mustafa , Pektemir İmdat Zafer
Published: August 2019

The temperature of a PV (photovoltaic) panel increases when it produces electricity but its electrical efficiency decreases when the temperature increases. In addition, the electrical efficiency of the PV panel is very limited. To increase the PV efficiency, the rest of the solar irradiance must be used, together with the temperature being kept at an optimum value. With this purpose, an experimental study was conducted. Firstly, two specific photovoltaic-thermal (PV/T) systems were designed. The first was the PV/T system which used only a water heat exchanger. The other one was the PV/T system that used a water and air heat exchanger. In the latter PV/T system, air passed through both the top of the PV panel and the bottom of it while water passed through only the bottom of the panel in a separate heat exchanger. In this way, the water and air absorbed the thermal energy of the panel by means of separate heat exchangers, simultaneously. In addition to the two systems mentioned above, an uncooled photovoltaic module was also designed in order to compare the systems. As a result, three different modules were designed. This study was conducted in a natural ambient environment and on days which had different climatic conditions. The thermal, electrical and overall efficiencies of each PV/T module were determined. The results were compared with the uncooled module electrical efficiency. The results showed that when water and air were used together, it was more efficient than single usage in a PV/T system. The thermal gain of the working fluids was also found to be fairly high and so, the gained energy could be used for different purposes. For example, hot air could be used in drying systems and air condition systems. Hot water could be used in hot water supply systems.
Thermo-Ultrasound-Based Sterilization Approach for the Quality Improvement of Wheat Plantlets Juice
Authors: Ahmed Zahoor , Manzoor Muhammad Faisal , Begum Nabila , Khan Abbas , Shah Imranullah , Farooq Umar , Siddique Rabia , Zeng Xin-An , Rahaman Abdul , Siddeeg Azhari
Published: August 2019

The impact of thermo–ultrasound (TU) on the quality of fresh wheat plantlets juice is described in this study. Fresh wheat plantlets juice was treated with TU using ultrasound (US) bath cleaner with different treatment variables, including power (70%, 420 W), frequency (40 kHz), processing time (20 and 40 min) and temperature (30, 45 and 60 °C) for the determination of free amino acids, minerals, microbial loads and bioactive compounds. The treatments have non-significant effects in ºBrix, pH, and titratable acidity while a significant increase in non-enzymatic browning, viscosity, and cloud value. The TU treatment at 30 °C for 20 and 40 min has achieved the highest value of total phenolics, flavonoids, total antioxidant capacity, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), carotenoids, anthocyanin contents, chlorophyll (a + b), minerals and free amino acids than other treatments as well as untreated sample. A lightly visible variation in the color was observed among all treatments. TU treatments also showed a significant impact on the reduction of microbial loads at 60 °C for 40 min. The verdicts revealed that TU at low temperature a viable option to improve the quality of wheat plantlets juice at an industrial scale as compared to alone.
Evaluation of Oleic Acid and Polyethylene Glycol Monomethyl Ether Conjugate (PEGylated Oleic Acid) as a Solubility Enhancer of Furosemide
Authors: Kalhapure Rahul S. , Bolla Pradeep Kumar , Boddu Sai HS. , Renukuntla Jwala
Published: August 2019

Poor aqueous solubility limits the therapeutic efficacy of many marketed and investigational drugs. Synthesis of new drugs with improved solubility is challenging due to time constraint and expenses involved. Therefore, finding the solubility enhancers for existing drugs is an attractive and profitable strategy. In this study, PEGylated oleic acid (OA-mPEG5000), a conjugate of oleic acid and mPEG5000 was synthesized and evaluated as a solubilizer for furosemide. OA-mPEG5000 was evaluated as a nanocarrier for furosemide by formulating polymersomes. Solubility of furosemide in milli-Q water and aqueous OA-mPEG5000 solution was determined using shake flask method. At 37 °C, the solubility of furosemide in OA-mPEG5000 (1% w/w) and milli-Q water was 3404.7 ± 254.6 µg/mL and 1020.2 ± 40.9 µg/mL, respectively. Results showed there was a 3.34-fold increase in solubility of furosemide in OA-mPEG5000 compared to water at 37 °C. At 25 °C, there was a 3.31-fold increase in solubilization of furosemide in OA-mPEG5000 (1% w/w) (90.0 ± 1.45 µg/mL) compared to milli-Q water (27.2 ± 1.43 µg/mL). Size, polydispersity index and zeta potential of polymersomes ranged from 85–145.5 nm, 0.187–0.511 and −4.0–12.77 mV, respectively. In-vitro release study revealed a burst release (71%) within 1 h. Significant enhancement in solubility and formation of polymersomes suggested that OA-mPEG5000 could be a good solubilizer and nanocarrier for furosemide.
Qualitative Fingerprint Analysis and Multidirectional Assessment of Different Crude Extracts and Essential Oil from Wild Artemisia santonicum L.
Authors: Ferrante Claudio , Zengin Gokhan , Menghini Luigi , Diuzheva Alina , Jekő József , Cziáky Zoltán , Recinella Lucia , Chiavaroli Annalisa , Leone Sheila , Brunetti Luigi , Lobine Devina , Senkardes Ismail , Mahomoodally Mohamad Fawzi , Orlando Giustino
Published: August 2019

Artemisia species are used as folk medicines in several countries. This work was aimed to shed more light on the effect of methanol, water, ethyl acetate extracts, and essential oil (EO) of A. santonicum on selected enzymes (cholinesterase, tyrosinase α-amylase, and α-glucosidase) as well of their antioxidant and pharmacological effects. The chemical profile of the essential oil was determined using gas chromatography coupled to mass spectrometry (GC-MS) analysis, while the extracts were chemically characterized by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Forty-nine constituents were identified and camphor (36.6%), 1,8-cineole (10.2%), α-thujone (10.1%), borneol (4.5%), and β-thujone (3.6%) were the major components. Overall, 45, 74, and 67 components were identified from the ethyl acetate, methanol, and water extracts, respectively. The EO and extracts showed significant antioxidant properties, in a cell-free model; particularly, methanol and water extracts revealed promising sources of antioxidant compounds. Additionally, we evaluated protective effects of EO and extracts in isolated rat colon tissue challenged with lipopolysaccharide (LPS), as an ex vivo model of colon inflammation, and human colon cancer HCT116 cell line. Particularly, we observed that, among all tested samples, A. santonicum ethyl acetate displayed the best pharmacological profile, being able to blunt LPS-induced levels of all tested biomarkers of inflammation and oxidative stress, including colon nitrites, lactate dehydrogenase, prostaglandin E2, and serotonin. Additionally, this extract was also able to reduce HCT116 cell viability, thus suggesting potential antiproliferative effects against colon cancer cells. Based on our results, A. santonicum has great potential for developing novel functional agents including pharmaceuticals, cosmeceuticals, and nutraceuticals.
Simultaneous Measurement of Ear Canal Movement, Electromyography of the Masseter Muscle and Occlusal Force for Earphone-Type Occlusal Force Estimation Device Development
Authors: Kurosawa Mami , Taniguchi Kazuhiro , Momose Hideya , Sakaguchi Masao , Kamijo Masayoshi , Nishikawa Atsushi
Published: August 2019

We intend to develop earphone-type wearable devices to measure occlusal force by measuring ear canal movement using an ear sensor that we developed. The proposed device can measure occlusal force during eating. In this work, we simultaneously measured the ear canal movement (ear sensor value), the surface electromyography (EMG) of the masseter muscle and the occlusal force six times from five subjects as a basic study toward occlusal force meter development. Using the results, we investigated the correlation coefficient between the ear sensor value and the occlusal force, and the partial correlation coefficient between ear sensor values. Additionally, we investigated the average of the partial correlation coefficient and the absolute value of the average for each subject. The absolute value results indicated strong correlation, with correlation coefficients exceeding 0.9514 for all subjects. The subjects showed a lowest partial correlation coefficient of 0.6161 and a highest value of 0.8286. This was also indicative of correlation. We then estimated the occlusal force via a single regression analysis for each subject. Evaluation of the proposed method via the cross-validation method indicated that the root-mean-square error when comparing actual values with estimates for the five subjects ranged from 0.0338 to 0.0969.
Lablab Purpureus Influences Soil Fertility and Microbial Diversity in a Tropical Maize-Based No-Tillage System
Authors: Dörr de Quadros Patricia , R. Martin Adam , Zhalnina Kateryna , Dias Raquel , Giongo Adriana , Fulthorpe Roberta , Bayer Cimelio , W. Triplett Eric , A. de O. Camargo Flávio
Published: August 2019

There are multiple mechanisms by which enhanced diversity of plant communities improves soil structure and function. One critical pathway mediating this relationship is through changes to soil prokaryotic communities. Here, nine different cropping systems were studied to evaluate how legume and grass cover crops influence soil fertility and microbial communities in a maize-based no tillage system. The soil’s bacterial and archaeal communities were sequenced (Illumina GAIIx, 12 replicates for treatment) and correlated with eight different soil features. The microbial community composition differed widely between planting treatments, with three primary “community types” emerging in multivariate space: (1) A community type associated with bare soil linked with low P, low pH, and high aluminum [Al]; (2) a community type associated with Lablab beans linked with high soil N, total organic carbon and other base cation concentrations, and high pH; and (3) a community type of all other non-lablab planting arrangements linked with higher soil P (relative to bare soil), but lower soil fertility (N and base cations). Lablab-based arrangements also expressed the highest microbial richness and alpha diversity. The inclusion of Lablab in maize-based cropping systems represents a potential alternative to reduce the use of chemical fertilizers and increase the chemical and biological quality in agricultural soils under the no-tillage system.
Loss and Damage Estimation for Extreme Weather Events: State of the Practice
Authors: Doktycz Charles , Abkowitz Mark
Published: August 2019

Extreme weather, climate-induced events that are episodic (e.g., hurricane, heatwave) or chronic (e.g., sea-level rise, temperature change) in nature, is occurring with increasing frequency and severity. This places a growing and time-sensitive need on the development and implementation of adaptation policies and practices. To motivate adaptive behavior, however, requires the ability to deliver improved risk-informed decision-making capability. At the crux of this challenge is the provision of full and accurate loss and damage accounting of the overall impact of an extreme weather event, enabling the business case to be made for adaptation investment. We define loss and damage as the manifestation of impacts associated with extreme weather that negatively affect human and natural systems. Progress in the development of adequate loss and damage accounting has been hampered by issues, such as discrepancies in conceptual frameworks, problems associated with data quantity and quality, and lack of standardized analysis methodologies. In this paper, we have discussed the conceptual basis for measuring loss and damage, reviewed the state of loss and damage data collection and modeling, and offered a narrative on the future direction of the practice.
A Cellular Automata Model of the Relationship between Adverse Events and Regional Infrastructure Development in an Active War Theater
Authors: Bozkurt Halil , Karwowski Waldemar , Çakıt Erman , Ahram Tareq
Published: August 2019

This study presents a cellular automata (CA) model to assist decision-makers in understanding the effects of infrastructure development projects on adverse events in an active war theater. The adverse events are caused by terrorist activities that primarily target the civilian population in countries such as Afghanistan. In the CA-based model, cells in the same neighborhood synchronously interact with one another to determine their next states, and small changes in iteration yield to complex formations of adverse event risks. The results demonstrate that the proposed model can help in the evaluation of infrastructure development projects in relation to changes in the reported adverse events, as well as in the identification of the geographical locations, times, and impacts of such developments. The results also show that infrastructure development projects have different impacts on the reported adverse events. The CA modeling approach can be used to support decision-makers in allocating infrastructure development funds to stabilize active war regions with higher adverse event risks. Such models can also improve the understanding of the complex interactions between infrastructure development projects and adverse events.
Artificial Anti-HIV-1 Immunogen Comprising Epitopes of Broadly Neutralizing Antibodies 2F5, 10E8, and a Peptide Mimic of VRC01 Discontinuous Epitope
Authors: Rudometov Andrey P. , Chikaev Anton N. , Rudometova Nadezhda B. , Antonets Denis V. , Lomzov Alexander A. , Kaplina Olga N. , Ilyichev Alexander A. , Karpenko Larisa I.
Published: August 2019

The construction of artificial proteins using conservative B-cell and T-cell epitopes is believed to be a promising approach for a vaccine design against diverse viral infections. This article describes the development of an artificial HIV-1 immunogen using a polyepitope immunogen design strategy. We developed a recombinant protein, referred to as nTBI, that contains epitopes recognized by broadly neutralizing HIV-1 antibodies (bNAbs) combined with Th-epitopes. This is a modified version of a previously designed artificial protein, TBI (T- and B-cell epitopes containing Immunogen), carrying four T- and five B-cell epitopes from HIV-1 Env and Gag proteins. To engineer the nTBI molecule, three B-cell epitopes of the TBI protein were replaced with the epitopes recognized by broadly neutralizing HIV-1 antibodies 10E8, 2F5, and a linear peptide mimic of VRC01 epitope. We showed that immunization of rabbits with the nTBI protein elicited antibodies that recognize HIV-1 proteins and were able to neutralize Env-pseudotyped SF162.LS HIV-1 strain (tier 1). Competition assay revealed that immunization of rabbits with nTBI induced mainly 10E8-like antibodies. Our findings support the use of nTBI protein as an immunogen with predefined favorable antigenic properties.
Sex-Specific Differences in HLA Antibodies after Pneumococcal Vaccination in Kidney Transplant Recipients
Authors: Lindemann Monika , Oesterreich Simon , Wilde Benjamin , Eisenberger Ute , Muelling Nils , Horn Peter A. , Heinemann Falko M. , Witzke Oliver
Published: August 2019

In transplant recipients vaccination against Streptococcus pneumoniae is recommended to reduce mortality from invasive pneumococcal disease. It is still debated if vaccination in transplant recipients triggers alloresponses. Therefore, it was our aim to define if vaccination with Prevenar 13®, a 13-valent, conjugated pneumococcal vaccine (Pfizer, New York, NY, USA) that acts T cell dependently, induces human leukocyte antigen (HLA) antibodies in clinically stable kidney transplant recipients. Forty-seven patients were vaccinated once with Prevenar 13® and HLA antibodies were determined prior to vaccination and at month 1 and 12 thereafter. In parallel, pneumococcal IgG antibodies were measured. Using Luminex™ Mixed Beads technology (One Lambda/Thermo Fisher, Canoga Park, CA, USA) we observed overall no change in HLA antibodies after vaccination. Pneumococcal antibodies increased significantly at month 1 (p < 0.0001) and remained elevated at month 12 (p < 0.005). A more detailed analysis of HLA antibodies showed that in 18 females HLA class I and II antibodies increased significantly at month 1 and 12 (p < 0.05); whereas in 29 males HLA class I and II antibodies tended to decrease. Using Luminex™ Single Antigen Beads assay, no de novo donor-specific HLA antibodies were detected after vaccination. In conclusion, the current data indicate that females may be more susceptible to the induction of (non-specific) HLA antibodies after vaccination.
System Dynamics Approach for Assessing the Behaviour of the Lim Reservoir System (Serbia) under Changing Climate Conditions
Authors: Stojkovic Milan , Simonovic Slobodan P.
Published: August 2019

Investigating the impact of climate change on the management of a complex multipurpose water system is a critical issue. The presented study focuses on different steps of the climate change impact analysis process: (i) Use of three regional climate models (RCMs), (ii) use of four bias correction methods (BCMs), (iii) use of three concentration scenarios (CSs), (iv) use of two model averaging procedures, (v) use of the hydrological model and (vi) use of the system dynamics simulation model (SDSM). The analyses are performed for a future period, from 2006 to 2055 and the reference period, from 1971 to 2000. As a case study area, the Lim water system in Serbia (southeast Europe) is used. The Lim river system consists of four hydraulically connected reservoirs (Uvac, Kokin Brod, Radojnja, Potpec) with a primary purpose of hydropower generation. The results of the climate change impact analyses indicate change in the future hydropower generation at the annual level from −3.5% to +17.9%. The change has a seasonal variation with an increase for the winter season up to +20.3% and decrease for the summer season up to −33.6%. Furthermore, the study analyzes the uncertainty in the SDSM outputs introduced by different steps of the modelling process. The most dominant source of uncertainty in power production is the choice of BCMs (54%), followed by the selection of RCMs (41%). The least significant source of uncertainty is the choice of CSs (6%). The uncertainty in the inflows and outflows is equally dominated by the choice of BCM (49%) and RCM (45%).
Antibiotic resistance profiles of vancomycin resistant enterococci in chicken meat samples
Authors: ONARAN Bahar , GÖNCÜOĞLU Muammer , BİLİR ORMANCI Fatma Seda
Published: August 2019

Antibiotic resistance is one of the serious threats to
global public health and food
safety today. Acquired antibiotic resistance in microorganisms arises from
prevalent use of antibiotics for human and animal medicine. Owing to the fact that Vancomycin Resistant Enteroccocci (VRE) is a vital
problem for public health, determination of the antibiotic resistance profiles
of Enterococcus spp. isolates have crucial importance as a part of the
farm to fork food safety. In the study, 120 retail chicken meat samples were
analyzed, and 36 (30%) of the samples were detected as Enterococcus spp.
positive. According to the results, the most prevalent species was E.
faecalis with a rate of 44.4% (16/36), followed by 27.8% (10/36) E.
faecium, 11.1% (4/36) E. durans, 2.8% (1/36) E. gallinarum
and 2.8% (1/36) E. casseliflavus. Antibiotic resistance profiles of the
verified Enterococcus spp. isolates were determined with disc diffusion
method in terms of eight different antibiotics. Among the Enterococcus
spp. isolates, 20 (55.5%) isolates were phenotypically resistant to vancomycin,
6 isolates (16.7%) were detected as vanA positive, 3 isolates (8.3%) were
detected as vanB positive, and one isolate (5%) showed high resistance
to vancomycin (MIC >256 µg/ml). Even though the observed percentages are
low, the observed resistance patterns are still of concern for public health.
Determination and stability of some international screening limited drugs in equine blood by LC-MS/MS
Authors: GÖKTAŞ Eylem Funda , KABİL Erol , GÜNEŞ Ertuğrul , KORKMAZ Serol , KADIOĞLU Tuba Asena , KURT CÜCÜ Ayşen
Published: August 2019

Stability of some
international screening limited therapeutic substances for doping control in
equine blood has been investigated with a validated method by using LC-MS/MS
after chemical hydrolysis with orthophosphoric acid. Whole blood samples were extracted
with a WAX cartridge on auto-SPE. Stability test of the drug substances
performed at the same time in working solution and matrix at +4°C, -20°C, +20°C
in dark and +20°C in light conditions for 1, 2, 3 and 4 weeks. For short-time
stability, the effects of 6 h storage at +55°C was also investigated. Repeated data were statistically analyzed with ANOVA.
All substances in working solution were stable at -20°C and +4°C. In the blood
matrix, higher temperatures (+20°C) in light and dark caused degradation of
substances at the end of four weeks and short-term study (P< 0.05). In
conclusion, these international screening limited substances in blood matrix
could be unstable related to temperature and storage time, although in working
solution they could be mostly stable in various temperatures for four weeks.
STRATEGI PROGRAMMING PROGRAM “METRO PAGI PRIMETIME” DALAM MEMPERTAHANKAN RATING
Authors: Putra Adi Pratama , Shintya Zaenina , Arisanty Melisa
Published: August 2019

Metro TV adalah stasiun  televisi swasta yang dimiliki oleh Surya Paloh yang menghadirkan program berita lebih banyak dibandingkan dengan stasiun lain. Metro TV stasiun televisi berita pertama yang menyiarkan berita dalam 3 bahasa, yaitu Indonesia, Inggris dan Mandarin. Salah satu program unggulan yang dimiliki Metro TV adalah program Metro Pagi Primetime tayang pada pukul 04.30-07.00 WIB, program ini menjadi unggulan karena dalam program ini terdapat dialog dengan narasumber. Selama ini rating yang di dapatkan program Metro Pagi Primetime selalu bertahan, Selain perusahaan televisi menampilkan informasi yang menarik dan aktual, hal tersebut tidak luput dari strategi programming dari perusahaan media demi menaikan rating perusahaan. Tujuan utama yang ingin diraih dari Programming televisi adalah untuk memaksimalkan jumlah penonton yang menjadi target bagi pemasang iklan dan rating perusahaan. Caranya adalah dengan memenuhi kepuasan penonton dengan menayangkan program acara yang sesuai dengan apa yang dibutuhkan atau diiginkan oleh audience.
PEMANFAATAN MEDIA SOSIAL SEBAGAI MEDIA PROMOSI
Authors: Puspitarini Dinda Sekar , Nuraeni Reni
Published: August 2019

Promosi adalah suatu hal yang harus dilakukan setiap perusahaan. Hal utama dalam promosi adalah membuat pesan yang persuasif yang efektif untuk menarik perhatian konsumen. Berdasarkan fakta tersebut, saat ini instagram tidak hanya digunakan sebagai sarana pemuas kebutuhan hiburan saja, tetapi juga sebagai media sosial yang mempunyai peluang besar untuk melakukan kegiatan bisnis, menyusul kesuksesannya sebagai media sosial yang diminati oleh pengguna. Happy Go Lucky house, pelopor concept store di Indonesia yang berdiri sejak 2008 mempromosikan produknya memakai instagram, tujuan penelitian ini adalah untuk mengetahui kegiatan promosi apa saja yang dilakukan oleh Happy Go Lucky house dalam akun media sosial instagram, dan mengetahui faktor-faktor apa yang membuat instagram dipilih sebagai media promosi yang aktif. Peneliti menarik kesimpulan bahwa Happy Go Lucky house melakukan pemanfaatan instagram dengan baik, dilihat dari kegiatan promosi yang dilakukan sangat beragam dan juga dapat memanfaatkan berbagai fitur yang tersedia.

 

Abstract

Promotion is an important thing to be done by a company. The key point in promotion is creating effective and persuasive message to attract buyers' attention. In line with that fact, instagram nowadays is used not only to fulfill users' entertainment needs, but also to be a social media with good opportunities to support business activities, following it's success to be a well known social media with lot of users. Happy Go Lucky house, pioneer of concept store fashion in Indonesia that established since 2008 have been using social media instagram as their product promotion platform. The objective of this research is to find out what kind of promotion activities doing by Happy Go Lucky house in social media account instagram. And also to find out the reasons why instagram is chosen as their active media promotion. The author concludes that Happy Go Lucky house doing well in using instagram, seen from their various promotions and also their ability of utilizing available features on it.
ЗМІНИ В ПРАВОВОМУ РЕГУЛЮВАННІ МІСЦЕВОГО САМОВРЯДУВАННЯ НА ЛІВОБЕРЕЖНІЙ УКРАЇНІ В УМОВАХ САМОДЕРЖАВСТВА (ХVIII–XIX СТОЛІТТЯ)
Authors: Бутирін Є.
Published: August 2019

Досліджується еволюція місцевого самоврядування на українських землях у ХVIII–ХІХ ст., яка відбувалася на тлі розвитку буржуазних відносин в умовах російського абсолютизму.
ЗАБОРОНЕНІ ДЛЯ ВИКОРИСТАННЯ В МІСЦЯХ ПОЗБАВЛЕННЯ ВОЛІ ПРЕДМЕТИ: ПОНЯТТЯ, СУТНІСТЬ ТА СУСПІЛЬНА НЕБЕЗПЕКА
Authors: Махніцька К.
Published: August 2019

У статті з’ясовано соціально-правову природу заборонених для засуджених до позбавлення волі предметів, їх роль і вплив на процес детермінації правопорушень і злочинів у сфері виконання покарань, а також запропоновано комплекс обґрунтованих заходів щодо запобігання цьому суспільно небезпечному явищу.
Effect of ultrasound on the dissolution of Mardin-Mazıdağı (Turkey) phosphate ore in dilute H3PO4 solutions
Authors: Sinirkaya M.
Published: August 2019

The dissolution of phosphate ore in dilute phosphoric acid solutions was investigated in both the absence and presence of ultrasound. Acid concentration, reaction temperature and reaction time were chosen as parameters. While the conversion fractions decreased with increasing temperature in dilute phosphoric acid in absence of ultrasound, these
rates increased with increasing temperature in the presence of ultrasound. In the experiments, the expected effects of ultrasound were observed, such as decrease in the thickness of the diffusion layer on the solid–liquid interfaces in the suspension, leading to increased surface area.
In vitro study on the antitumor activity of Tanacetum vulgare L. extracts
Authors: Vasileva A. M. , Iliev I. A. , Lozanov V. S. , Dimitrova M. B. , Mitev V. I. , Ivanov I. P.
Published: August 2019

The major nonvolatile compounds derived from extracts and fractions from Tanacetum vulgare L. flowers were determined by LC–HRMS. Major compounds in the crude extract were determined to be: six hydroxycinnamoyl quinic acids with 4,5-dicaffeoylquinic acid and twelve flavonoids and their derivatives, six of which were in the form of flavonoid-O-glucuronides. Generally, the major flavonoid aglycone in tansy was luteolin. Extracts and fractions were tested under in vitro conditions in nine cell lines - one control non-tumorogenic and eight tumor lines, whereby antitumor activity was observed after 72 hours of incubation with the aforementioned substances as determined by an MTT assay. The obtained results show the highest selectivity index for the ethyl acetate extract from Flores Tanaceti (EAFT) and for the ethyl acetate fraction of the crude extract (EACE). EAFT extract was found to exert the highest antitumor effect, followed by EACE. From the above results it becomes evident that ethyl acetate extracts of T. vulgare contain substances with high selective activity against tumor cells.
Design, synthesis, structural characterization and cell cytotoxicity of a new derivative poly(maleic anhydride-co-vinyl acetate)/miltefosine polymer/drug conjugate
Authors: Karakus G. , Akin Polat Z. , Karahan M.
Published: August 2019

In recent years, polymeric systems are selected as biomaterials because of their desired biocompatible properties and easy design/preparation of a number of different structures with lower toxicity and good solubility. Nontoxic polymeric drug carrier, maleic anhydride-co-vinyl acetate copolymer (MAVA), was prepared via free-radical chain polymerization at 80±0.1 ºC. MEK (methyl ethyl ketone) and BPO (benzoyl peroxide) were used as the organic medium and radical initiator, respectively. Copolymer was conjugated with a broad-spectrum antimicrobial agent, miltefosine (MF, an oral drug in the treatment of leishmaniosis), Impavido® and Miltex®, 1:1 molar ratio of copolymer:drug for 48 h at 60 °C in aqueous medium in presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC). Fourier transform infrared (FTIR) and nuclear magnetic resonance (1H- and 31P-NMR) were used to characterize the structure of the copolymer and MAVA/MF conjugate. Molecular weights were measured via size-exclusion chromatography (SEC). Results, obtained from the spectroscopic and SEC analysis, verified that conjugation was successfully carried out with good water-solubility. WST-1 cytotoxicity tests, 24 h by quantitative analysis, were carried out for copolymer, miltefosine, and MAVA/MF. The cytotoxicity values, by comparing with control group, were found statistically significantly different (P<0.05). MAVA/MF copolymer/drug couple was successfully designed with lower cytotoxicity than the free drug (MF).
Assessment of Life Satisfaction among Physiotherapists from Selected Hospitals in South-Western Nigeria
Authors: Ojoawo Adesola O , Igudia Joy O , Mbada Chidozie E , Onigbinde Ayodele, T , Adedoyin Rufus A
Published: August 2019

Background: Life satisfaction of an individual is strongly associated with factors such as income, educational status, family and occupation. There is a dearth of studies on job and life satisfaction for physiotherapists, especially in Nigeria.Objective: This study assessed life satisfaction for physiotherapists from selected hospitals in South-Western Nigeria.Methods: Seventy-six physiotherapists from seven purposively selected hospitals who met the inclusion criteria responded to the study. The Life Satisfaction Scale Questionnaire (LSSQ) was used as the research instrument. The LSSQ is a two-section questionnaire. Section A comprises 6 items designed to assess demographic information of the respondents while section B consists of 29 items, designed to measure the life satisfaction of the respondents. The questions on perceived level of satisfaction in different domains are on a 7-point scale ranging from 7 (strongly agree) to 1 (strongly disagree) for each question. Descriptive and inferential statistics were used to analyze the data.Results: The response rate of the study was 80%. The mean total life satisfaction of the respondents was 151 – 180. There was a significant difference in total life satisfaction scores at p < 0.001 between the single and married respondents, first degree and postgraduates and between entry physiotherapist and senior physiotherapist. There was a significant association between level of life satisfaction and level of education, at p < .05. There also was a significant association, p < 001, between the level of life satisfaction and age, marital status and cadre.Conclusion: Life satisfaction of clinical physiotherapists in South-Western Nigeria was high and is associated with marital status, postgraduate qualification and in a higher professional cadre. This implies that physiotherapists in South-Western Nigeria are in a condition that promotes high productivity and efficiency and therefore physiotherapists are more likely to not migrate out of the country.
Exploring the Relationship among University Teachers' Emotional Intelligence,
Emotional Labor Strategies, and Teaching Satisfaction
Authors: Arif Imtiaz , Umer Amna , Kazmi Syeda Wajiha , Khalique Muhammad
Published: August 2019

In this study we investigated the impact of emotional intelligence on university teacher satisfaction with emotional labor strategies as mediators. In order to evaluate this relationship, three scales were used WLEIS, TELSS and TSS for emotional intelligence, emotional labor strategies and teaching satisfaction respectively. The data was collected from 449 university teachers including both adjunct and permanent. Structured equation model and phantom model were utilized to analyze the mediating effect of emotional labor strategies on emotional intelligence and teaching satisfactions. In this study second-order factor of emotional intelligence was used and findings showed that emotional intelligence has a significant positive relationship with teaching satisfaction whereas surface acting and deep acting have negative impact on teaching satisfaction. However, expressions of naturally felt emotions have a positive insignificant relationship with the dependent variable. Surface acting and deep acting when worked as a mediator, they resulted in negative association between emotional intelligence and teaching satisfaction. Generally, this research revealed that
emotional intelligence was critically important for the satisfaction of teachers with their work. This study would also help the university management to incorporate those emotional labor strategies which would in turn enhance teaching satisfaction.

Keywords: Emotional Intelligence, Emotional labor strategies, Teacher satisfaction, University, Pakistan.
FILOSOFI PENDIDIKAN YANG INTEGRAL DAN HUMANIS DALAM PERSPEKTIF MANGUNWIJAYA
Authors: Dewantara Agustinus Wisnu
Published: August 2019

Mangunwijaya introduced humanist educational philosophy and integrality. Education, according to Mangunwijaya, must create a climate that allows the child to divide themselves into an independent person. Education should aim to deliver learners in recognizing and developing human potential itself into a whole (not just the brain, but all aspects of humanity: skilled, intelligent, piety, solidarity, capable, and responsible). An education system should be humane. That is, a system of compulsory education to respect human dignity, particularly in the person of the child. Schools should thus be understood as a division of talent and togetherness with others. Consequently, the teaching system should not be alienated from the life of the concrete. That is, not merely biased cognitive, intellectualist or mere romantic extreme, but really develop talent, art, language, manners, morals, taste, religiosity, and social life.