bluuebunny/crossref_metadata_2025 huggingface dataset:
Subclinical Left Ventricular Dysfunction in Children and Adolescence With Thalassemia Intermedia
Authors: Isa Tafreshi Roya , Radgoodarzi Mohammad , Arjmandi Rafsanjani Kadijeh , Soheilipour Fahimeh
Published: June 2022

Background

Cardiac complications are important causes of morbidity in patients with thalassemia intermedia (TI). We aimed to assess left ventricular (LV) function, using new tissue Doppler imaging (TDI) indices, in order to diagnose early ventricular impairment in asymptomatic children and adolescence with the TI.

Materials and Methods

We investigated possible differences in echocardiographic systolic and diastolic parameters between a population of 28 asymptomatic patients (mean age, 13.6 ± 5.7 years) and 35 age-matched healthy control members. All of them underwent 2-D, pulsed Doppler, and tissue Doppler echocardiographic studies for the assessment of the LV mass, Trans-mitral velocities, mitral annular systolic and diastolic velocities, myocardial performance index (MPI), and myocardial acceleration during isovolumic contraction (IVA). The cardiac iron load was estimated by magnetic resonance imaging T2*.

Results

Left ventricular hypertrophy (LVH) was found in 13 (46.4%) patients. We found significantly reduced TDI-derived peak systolic myocardial velocity (s′) in patients, whereas no significant difference was identified between the patients and control group members when the IVA was compared. The ratio of peak mitral inflow velocity to annular early diastolic velocity (E/e′) of the mitral valve as an index of the diastolic function was significantly higher in patients (9 ± 1 vs. 6 ± 1, p < 0.05). Choosing a TDI-derived MPI > 0.33 as a cutoff point, the global LV dysfunction was detected with a sensitivity of 78% and a specificity of 80%. The patients with LVH significantly exhibited higher values of TDI-MPI and lower values of s′ velocity and IVA when compared against the subjects with normal LV mass.

Conclusion

Subtle LV systolic and diastolic dysfunction develops early in young patients with the TI who have normal cardiac iron concentration. Moreover, LV remodeling as a main cardiac adaptive response plays a principal role in developing myocardial impairment.
Screening of Hydrocarbon-Stapled Peptides for Inhibition of Calcium-Triggered Exocytosis
Authors: Lai Ying , Tuvim Michael J. , Leitz Jeremy , Peters John , Pfuetzner Richard A. , Esquivies Luis , Zhou Qiangjun , Czako Barbara , Cross Jason B. , Jones Philip , Dickey Burton F. , Brunger Axel T.
Published: June 2022

The so-called primary interface between the SNARE complex and synaptotagmin-1 (Syt1) is essential for Ca2+-triggered neurotransmitter release in neuronal synapses. The interacting residues of the primary interface are conserved across different species for synaptotagmins (Syt1, Syt2, Syt9), SNAP-25, and syntaxin-1A homologs involved in fast synchronous release. This Ca2+-independent interface forms prior to Ca2+-triggering and plays a role in synaptic vesicle priming. This primary interface is also conserved in the fusion machinery that is responsible for mucin granule membrane fusion. Ca2+-stimulated mucin secretion is mediated by the SNAREs syntaxin-3, SNAP-23, VAMP8, Syt2, and other proteins. Here, we designed and screened a series of hydrocarbon-stapled peptides consisting of SNAP-25 fragments that included some of the key residues involved in the primary interface as observed in high-resolution crystal structures. We selected a subset of four stapled peptides that were highly α-helical as assessed by circular dichroism and that inhibited both Ca2+-independent and Ca2+-triggered ensemble lipid-mixing with neuronal SNAREs and Syt1. In a single-vesicle content-mixing assay with reconstituted neuronal SNAREs and Syt1 or with reconstituted airway SNAREs and Syt2, the selected peptides also suppressed Ca2+-triggered fusion. Taken together, hydrocarbon-stapled peptides that interfere with the primary interface consequently inhibit Ca2+-triggered exocytosis. Our inhibitor screen suggests that these compounds may be useful to combat mucus hypersecretion, which is a major cause of airway obstruction in the pathophysiology of COPD, asthma, and cystic fibrosis.
β2-Microglobulin is a Novel and Reliable Biomarker for Predicting Ischemic Stroke Recurrence: A Prospective Cohort Study
Authors: Hu Fu-yong , Wu Wentao , Liu Qiuwan , Wu Juncang , Guo Hualing , Yang Jing , Wu Zhuqing , Jiang Ke , Wang Guoping , Qian Yu , Ge Wei , Qun Sen
Published: June 2022

Immune and inflammatory mechanisms play key roles in the development and outcome of acute ischemic stroke (AIS). β2-Microglobulin (β2M) is the light chain of major histocompatibility complex-1 (MHC-1), which can directly and quickly reflect the immune and inflammatory state of the body. Previous studies have shown a close relationship between β2M and AIS, but its relationship with the recurrence of AIS has not been reported. This study attempted to explore the relationship between β2M and the recurrence of AIS. A single-center AIS cohort involving 135 patients was followed for approximately 26–46 months. Clinical and laboratory data from the patients were collected when hospitalized. The endpoint was the occurrence of recurrent AIS after patients were discharged. Propensity score matching was used to match cohort groups. Cox regression analysis was used to predict risk factors for recurrent AIS, and receiver operating characteristic curve (ROC) analysis was used to calculate the optimal cutoff value for discriminating recurrence in patients with AIS. The rate of recurrence was 29.6% [95% CI, 21.8%–37.3%] in the follow-up group. Patients with higher levels of serum β2M had a higher risk of AIS recurrence than patients with lower levels of β2M (adjusted hazard ratio, 3.214 [95% CI, 1.557–6.633]; adjusted hazard ratio after matching, 5.831, [95% CI, 2.052–16.572]). A β2M value of 2.31 mg/L was calculated by ROC analysis as the optimal cutoff value for AIS recurrence (area under the curve 0.770, [95% CI, 0.687–0.853]). As a quick responder to the body’s immune and inflammatory states, β2M may be a novel and reliable biomarker in predicting AIS recurrence.
Therapeutic Efficacy of Carvacrol-Loaded Nanoemulsion in a Mouse Model of Schistosomiasis
Authors: Xavier Edilaine S. , de Souza Rafael L. , Rodrigues Vinícius C. , Melo Camila O. , Roquini Daniel B. , Lemes Bruna L. , Wilairatana Polrat , Oliveira Elquio E. , de Moraes Josué
Published: June 2022

Since praziquantel is the only drug available to treat schistosomiasis, a neglected parasitic disease that affects more than 240 million people worldwide, there is an urgent demand for new antischistosomal agents. Natural compound-loaded nanoparticles have recently emerged as a promising alternative for the treatment of schistosomiasis. Carvacrol is an antimicrobial monoterpene present in the essential oil extracted from several plants, especially oregano (Origanum vulgare). In this study, a carvacrol nanoemulsion (CVNE) was prepared, characterized, and administered orally (200 mg/kg) in a mouse infected with either immature (prepatent infection) or adult (patent infection) Schistosoma mansoni. For comparison, data obtained with an unloaded nanoemulsion (blank formulation), free carvacrol, and the drug of reference praziquantel are also presented. CVNE was more effective than free carvacrol in reducing the worm burden and egg production in both patent and prepatent infections. Favorably, CVNE had a high effect in terms of reducing the number of worms and eggs (85%–90%) compared with praziquantel (∼30%) in prepatent infection. In tandem, carvacrol-loaded nanoemulsion markedly improved antischistosomal activity, showing efficiency in reducing worm and egg burden, and thus it may be a promising delivery system for the treatment of schistosomiasis.
Molecular Pharmacology of P2X Receptors: Exploring Druggable Domains Revealed by Structural Biology
Authors: Oken Adam C. , Krishnamurthy Ipsita , Savage Jonathan C. , Lisi Nicolas E. , Godsey Michael H. , Mansoor Steven E.
Published: June 2022

Extracellular ATP is a critical signaling molecule that is found in a wide range of concentrations across cellular environments. The family of nonselective cation channels that sense extracellular ATP, termed P2X receptors (P2XRs), is composed of seven subtypes (P2X1-P2X7) that assemble as functional homotrimeric and heterotrimeric ion channels. Each P2XR is activated by a distinct concentration of extracellular ATP, spanning from high nanomolar to low millimolar. P2XRs are implicated in a variety of physiological and pathophysiological processes in the cardiovascular, immune, and central nervous systems, corresponding to the spatiotemporal expression, regulation, and activation of each subtype. The therapeutic potential of P2XRs is an emerging area of research in which structural biology has seemingly exceeded medicinal chemistry, as there are several published P2XR structures but currently no FDA-approved drugs targeting these ion channels. Cryogenic electron microscopy is ideally suited to facilitate structure-based drug design for P2XRs by revealing and characterizing novel ligand-binding sites. This review covers structural elements in P2XRs including the extracellular orthosteric ATP-binding site, extracellular allosteric modulator sites, channel pore, and cytoplasmic substructures, with an emphasis on potential therapeutic ligand development.
17β-Estradiol Treatment Improves Acetylcholine-Induced Relaxation of Mesenteric Arteries in Ovariectomized UC Davis Type 2 Diabetes Mellitus Rats in Prediabetic State
Authors: Razan Md Rahatullah , Akther Farjana , Islam Rifat A. , Graham James L. , Stanhope Kimber L. , Havel Peter J. , Rahimian Roshanak
Published: June 2022

We recently reported sex differences in mesenteric arterial function of the UC Davis type-2 diabetes mellitus (UCD-T2DM) rats as early as the prediabetic state. We reported that mesenteric arteries (MA) from prediabetic male rats exhibited a greater impairment compared to that in prediabetic females. However, when females became diabetic, they exhibited a greater vascular dysfunction than males. Thus, the aim of this study was to investigate whether the female sex hormone, estrogen preserves mesenteric arterial vasorelaxation in UCD-T2DM female rats at an early prediabetic state. Age-matched female Sprague Dawley and prediabetic (PD) UCD-T2DM rats were ovariectomized (OVX) and subcutaneously implanted with either placebo or 17β-estradiol (E2, 1.5 mg) pellets for 45 days. We assessed the contribution of endothelium-derived relaxing factors (EDRF) to acetylcholine (ACh)-induced vasorelaxation, using pharmacological inhibitors. Responses to sodium nitroprusside (SNP) and phenylephrine (PE) were also measured. Additionally, metabolic parameters and expression of some targets associated with vascular and insulin signaling were determined. We demonstrated that the responses to ACh and SNP were severely impaired in the prediabetic state (PD OVX) rats, while E2 treatment restored vasorelaxation in the PD OVX + E2. Moreover, the responses to PE was significantly enhanced in MA of PD OVX groups, regardless of placebo or E2 treatment. Overall, our data suggest that 1) the impairment of ACh responses in PD OVX rats may, in part, result from the elevated contractile responses to PE, loss of contribution of endothelium-dependent hyperpolarization (EDH) to vasorelaxation, and a decreased sensitivity of MA to nitric oxide (NO), and 2) the basis for the protective effects of E2 may be partly attributed to the elevation of the NO contribution to vasorelaxation and its interaction with MA as well as potential improvement of insulin signaling. Here, we provide the first evidence of the role of E2 in protecting MA from early vascular dysfunction in prediabetic female rats.
Systematic Analysis of Tobacco CrRLK1L Family Genes and Functional Identification of NtCrRLK1L47 in Environmental Stresses
Authors: Li Xiaoxu , Guo Cun , Wang Qi , Li Zhiyuan , Cai Jun , Wu Dousheng , Li Yangyang , Yang Aiguo , Guo Yongfeng , Gao Junping , Wen Liuying , Pu Wenxuan
Published: June 2022

The Catharanthus roseus RLK1-like (CrRLK1L) family is involved in the regulation of plant reproduction, growth and development, cell wall integrity sensing, as well as responses to both biotic and abiotic stress conditions. Extraordinary progress has been made in elucidating the CrRLK1L family receptor kinases–mediated signaling pathway, while limited research addressed the functions of CrRLK1L proteins in tobacco. In this study, we identified and analyzed 48 NtCrRLK1L members from the tobacco genome. The newly identified NtCrRLK1L members were divided into seven groups together with the Arabidopsis CrRLK1L members. The syntenic analysis revealed that four pairs of NtCrRLK1L genes were predicted to have arisen from segmental duplication events. Expression profiling showed that the NtCrRLK1L genes were expressed in various tissues, and most NtCrRLK1L genes were induced by salt and drought stress conditions. Notably, NtCrRLK1L47 was upregulated under drought and salinity stresses, and the NtCrRLK1L47-GFP fusion protein was located in the cell membrane. Furthermore, overexpression of the NtCrRLK1L47 gene enhanced the salt tolerance in tobacco seedlings.
Genome-Wide Identification of Genes Related to Biosynthesis of Phenolic Acid Derivatives in Bletilla striata at Different Suspension Culture Stages
Authors: Liu Houbo , Huang Ceyin , Li Qingqing , Wang Mufei , Xiao Shiji , Shi Junhua , He Yihuai , Wen Weie , Li Lin , Xu Delin
Published: June 2022

To screen the genes regulating the biosynthesis of phenolic acid derivatives from the genome of Bletilla striata, we designed a suspension culture system to sample the cells for the following experiments. The contents of four phenolic acid derivatives were determined by high-performance liquid chromatography, and several full-length transcriptome sequencings of RNA samples at 10 time points were performed for bioinformatics analysis. The correlation analysis was used to identify and verify the key DEGs involved in the biosynthesis of the four phenolic acid derivatives. The results showed that the contents of p-hydroxybenzylalcohol (HBA), Dactylorhin A, Militarine, and Coelonin peaked at 33 days postinoculation (Dpi), 18 Dpi, 39 Dpi, and 39 Dpi of the culture system, respectively. Based on transcriptome data, 80 DEGs involved in the biosynthesis of phenolic acid derivatives were obtained. The KEGG pathway enrichment analysis classified them mostly into five metabolic pathways: phenylpropane biosynthesis, starch and sucrose metabolic, cyanoamino acid metabolism, gluconeogenesis and glycolysis, and phenylalanine metabolism. qPCR analysis revealed that the relative gene expression levels were consistent with the overall trend of transcriptome sequencing results. Among them, 14, 18, 23, and 41 unigenes were found to be involved in the synthesis of HBA, Dactylorhin A, Coelonin, and Militarine, respectively. These unigenes laid a solid foundation for elucidating the biosynthesis mechanism of phenolic acid derivatives in suspension cells of B. striata.
Young Children’s Conceptualisations of Kindness: A Thematic Analysis
Authors: Perkins Nicole , Smith Patrick , Chadwick Paul
Published: June 2022

Although there is much interest in the development of prosocial behaviour in young children, and many interventions that attempt to cultivate kindness in children, there is a paucity of research exploring children’s lived experiences of kindness and including their voices. In this study, children’s understanding of kindness is approached through qualitative interviews using puppets. Interviews were conducted with 33 children aged 5-6 years in 3 schools in the United Kingdom. Through thematic analysis, 4 themes were developed: (a) doing things for others, (b) relating with others, (c) rules and values, and (d) kindness affects us. These themes are examined in light of current thinking on prosocial and sociomoral development, and several key insights are highlighted, including types of prosocial behaviour, social connection, kindness-by-omission and defending, in-group bias, universal kindness versus personal safety, self-image, and a desire to improve the condition of society. These findings have implications for future research on prosocial development and for the design of kindness-based interventions, as well as providing an ecologically valid method of inquiry for use with young children.
Is Social Distancing Law the New Normal? Forced Shift to Media Online Learning and Its Effectiveness: A Moderating Role of Student Engagement During the Pandemic of COVID-19
Authors: Liu Qing , Mo Shuwen
Published: June 2022

The author intends to investigate the role of social distancing laws in the new normal as well as the effectiveness of forced shift to media online learning. This research indicates that student involvement had a moderating influence during the epidemic. This study is based on social learning theory (SLT), which endeavors to emulate the behavior, perceptions, and emotions of other individuals. The data were obtained from various Chinese universities. We gathered data utilizing the stratified sample approach as well as Google Form. A total of 256 students enrolled in a variety of programs at Chinese universities completed a questionnaire for this investigation. The direct, mediating, and moderating effects of the variables were evaluated using partial least square structural equation modeling in this study (PLS-SEM), using the Smart-PLS software 3.0. According to the findings, forced shift to media online learning acts as a mediator between the lack of social interaction, perceived harm, perception of maintaining social distancing, and the law, and the effectiveness of media online learning has a significant effect on the effectiveness of media online learning. In addition, student engagement appears to be a moderator between the forced shift to media online learning and its effectiveness. A future study might concentrate on gaining a deeper understanding of the numerous online engagement-promoting behaviors. Teachers must go above and above to provide themes that enable pupils to connect while learning. Instructors and educational institutions will benefit from the research since it will allow them to define methods for boosting social interaction in online learning and analyze methods for enhancing the efficacy of media in online learning.
Is It Personal? The Effect of Personal vs. Occupational Trauma on PTSD Symptom Severity in Emergency Responders
Authors: Wild Jennifer , Chang Tingyee E.
Published: June 2022

Emergency responders are exposed to potentially traumatic events in their line of work and as such, are at increased risk of developing post-traumatic stress disorder (PTSD). Little is known about the characteristics of trauma associated with PTSD symptoms in this population. This study analyzed the self-reported worst traumatic event on the PTSD checklist for DSM-5 for a sample ofN= 610 emergency responders, working as police officers, paramedics, firefighters or search and rescue personnel. Sufficient information was available to code 98% (N= 603) participants' trauma; 84% (N= 509) met DSM-V criterion A trauma. Of the participants reporting criterion A trauma, 56.9% (N= 290) participants reported being most affected by a traumatic event that occurred in their personal lives, 41.5% (N= 211) participants reported being most affected by a work-related traumatic event and 1.6% (N= 8) reported criterion A events that were work-related and had occurred prior to their role as an emergency responder (e.g., combat). Paramedics were significantly more likely to report occupational trauma as their worst event whereas police officers, firefighters, and search and rescue workers reported personal trauma as their worst event. Personal trauma was associated with significantly greater PTSD symptom severity than occupational trauma. Emergency responders identifying as women were significantly more likely to report personal than work-related trauma as their index event and men were more likely to report work-related than personal trauma as being linked to their PTSD symptoms. The results underscore the need to consider the broader context of trauma in the emergence of PTSD symptoms in emergency workers.
Network Analysis of the Relationship Between Trait Depression and Impulsiveness Among Youth
Authors: Zhang Jingxuan , Li Kuiliang , Xue Yitong , Feng Zhengzhi
Published: June 2022

Objective

Both impulsiveness and trait depression are the trait-level risk factors for depressive symptoms. However, the two traits overlap and do not affect depressive symptoms independently. This study takes impulsiveness and trait depression into a whole construct, aiming to find the complex associations among all facets and explore their relative importance in a trait network. It can help us find the key facets that need consideration in preventing depression.

Materials and Methods

We used the Barratt Impulsiveness Scale (BIS) and Trait Depression Scale (T-DEP) as measuring tools, conducted network analysis, and applied the Graphic Least Absolute Shrinkage and Selection Operator (GLASSO) algorithm to estimate the network structure and compute the linkage and centrality indexes. The accuracy and stability of the indexes were estimated through bootstrapping. All the computations were performed by R script and packages.

Results

We found that “trait anhedonia” was connected with “non-planning” and “cognitive” impulsiveness, while “trait dysthymia” was connected with “motor” impulsiveness. “Cognitive” impulsiveness had a statistically significant higher expected influence than “motor” impulsiveness and had the trend to be dominant in the network. “Trait dysthymia” had a statistically significant higher bridge expected influence than “cognitive” impulsiveness and had the trend to be the key facet linking impulsiveness with trait depression. “Non-only children” had higher network global strength than “only children.” All indexes were accurate and stable.

Conclusion

The present study confirms the complex associations among facets of trait depression and impulsiveness, finding that “cognitive” impulsiveness and “trait dysthymia” are the two key factors in the network. The results imply that different facets of impulsiveness should be considered respectively regarding anhedonia and dysthymia. “Cognitive” impulsiveness and “trait dysthymia” are critical to the prevention of depression.
Impact of Xpert MTB/RIF on Outcomes of Adults Hospitalized With Spinal Tuberculosis: Findings From a Comparative Cohort in Beijing, China
Authors: Fan Jun , An Jun , Shu Wei , Tang Kai , Shang Yuanyuan , Xue Yi , Qin Shibing , Pang Yu
Published: June 2022

Background

Spinal tuberculosis (TB) is one of the most common forms of extrapulmonary tuberculosis, causing increased morbidity and lifelong disabilities. Here, we conducted a retrospective study to determine the impact on patient outcomes of the Xpert MTB/RIF test vs. phenotypical drug susceptibility testing for spinal TB.

Methods

In-patients with spinal TB were enrolled in 2013 and 2017 at Beijing Chest Hospital. Data were collected from an electronic patient record system that documented demographic and clinical characteristics. All the patients were routinely followed-up at 1, 3, 6, 9, and 12 months after surgery during outpatient treatment.

Results

A total of 361 patients affected by spinal TB were enrolled in our analysis, including 178 patients in 2013 and 183 patients in 2017. In 2013, the cumulative postoperative recurrence rate of patients with spinal TB was 23% (41/178), which was significantly higher than that in 2017 (8.2%, 15/183, P < 0.001). Additionally, the patients with spinal TB diagnosed in 2013 relapsed significantly sooner than those in 2017 (P < 0.001). In the multivariate analysis, rifampicin (RIF) resistance was associated with the recurrence of spinal TB. The turnaround time of Xpert ranged from 1 to 3 days, with a median of 1 day (IQR: 1–2). For the phenotypic drug susceptibility test (pDST)-based algorithm, the median turnaround time was 67 days, considerably longer than that of the Xpert-based algorithm (P < 0.001).

Conclusion

The RIF resistance is an independent risk factor for postoperative recurrence in patients with spinal TB. Early detection of RIF resistance due to the application of Xpert is an effective strategy to reduce spinal TB recurrence.
Global Burden and Trends of Norovirus-Associated Diseases From 1990 to 2019: An Observational Trend Study
Authors: Zhang Xiaobao , Chen Can , Du Yuxia , Yan Danying , Jiang Daixi , Liu Xiaoxiao , Yang Mengya , Ding Cheng , Lan Lei , Hecht Robert , Yang Shigui
Published: June 2022

Introduction

As an important pathogen causing diarrheal diseases, the burden and change in the death rate of norovirus-associated diseases (NADs) globally are still unknown.

Methods

Based on global disease burden data from 1990 to 2019, we analyzed the age-standardized death rate (ASDR) of NADs by age, region, country, and Socio-Demographic Index (SDI) level. The discrete Poisson model was applied in the analysis of NADs' spatiotemporal aggregation, the Joinpoint regression model to analyze the trend of death burden of NADs over 30 years, and a generalized linear model to identify the risk factors for the death rate from NADs.

Results

The ASDR of NADs significantly decreased by a factor of approximately 2.7 times, from 5.02 (95% CI: 1.1, 11.34) in 1990 to 1.86 (95% CI: 0.36, 4.16) in 2019 [average annual percent change (AAPC) = −3.43, 95% CI: −3.56, −3.29]. The death burden of NADs in 2019 was still highest in African regions despite a great decline in recent decades. However, the ASDR in high SDI countries presented an uptrend [0.12 (95% CI: 0.03, 0.26) in 1990 and 0.24 (95% CI: 0.03, 0.53) in 2019, AAPC = 2.52, 95% CI: 2.02–3.03], mainly observed in the elderly over 70 years old. Compared to children under 5 years old, the 2019 death rate of elderly individuals over 80 years old was much higher in high SDI countries. The generalized linear model showed that factors of the number of physicians (RR = 0.67), the proportions of children under 14 years old (RR = 1.21), elderly individuals over 65 years old (RR = 1.13), educational level (RR = 1.03) and urbanization proportion (RR = 1.01) influenced the ASDR of NADs.

Conclusions

The death burden of NADs has remained high in developing regions over the last three decades and has increased among the elderly in countries with high SDI levels, even though the global trend in NAD-associated deaths has decreased significantly in the past three decades. More effective public health policies against NADs need to be implemented in high SDI regions and for the elderly.
Psychometric Properties and Measurement Invariance of the Chinese Version of the Brief Assessment of Impaired Cognition Questionnaire in Community-Dwelling Older Adults
Authors: Li Shaojie , Cui Guanghui , Jørgensen Kasper , Cheng Zimi , Li Zihao , Xu Huilan
Published: June 2022

This study aimed to verify the Chinese version of the Brief Assessment of Impaired Cognition Questionnaire (C-BASIC-Q), and provide a new tool for the future large-scale epidemiological investigation of cognitive function in China. From March to May 2021, a cross-sectional study of 2,144 Chinese community-dwelling older adults (men = 1,075, mean age = 72.01 years, SD = 6.96 years, ranging from 60–99 years) was conducted in Jinan. Exploratory and confirmatory factor analyses were performed to evaluate the factor structure of the C-BASIC-Q. Convergent validity was evaluated by correlations with the Mini-Mental State Examination (MMSE). Internal consistency and test-retest reliability were evaluated using Cronbach's alpha and retest correlations in a sub-sample (n = 129). Linear regression was used to analyze the impact of demographic factors on the MMSE and C-BASIC-Q scores. Measurement invariance was evaluated using a multi-group confirmatory factor analysis. The mean C-BASIC-Q score was 15.94 (SD = 3.43). Factor analysis suggested a three-factor structure of C-BASIC-Q (self-report, orientation, and informant report). The C-BASIC-Q score was significantly positively associated with the MMSE score, showing good convergent validity. Cronbach's alpha of the C-BASIC-Q was 0.862, and the test-retest correlation coefficient was significant (r = 0.952, p < 0.001), indicating good internal consistency and test-retest reliability. Measurement invariance analysis showed that C-BASIC-Q had configural, metric, and scalar invariance across sex, age, residence, education level and marital status. C-BASIC-Q was less affected by age, residence, education, and marital status than the MMSE. In summary, the C-BASIC-Q had good reliability, validity, and measurement invariance, and is a valid tool for evaluating cognitive functioning in Chinese community-dwelling older adults.
The Burden of Trachea, Bronchus, and Lung Cancer Attributable to Occupational Exposure From 1990 to 2019
Authors: Li Haifeng , Guo Jingwen , Liang Hongsen , Zhang Ting , Zhang Jinyu , Wei Li , Shi Donglei , Zhang Junhang , Wang Zhaojun
Published: June 2022

Objectives

Occupational exposure to carcinogens is associated with trachea, bronchus, and lung (TBL) cancer. The objective of this study was to provide global and regional estimates of the burden of TBL cancer associated with occupational carcinogens (OCs) between 1990 and 2019.

Methods

Age-standardized mortality rates (ASMR) and age-standardized disability-adjusted life years (DALYs) rates (ASDR) of TBL cancer related to exposure to OCs at the global and regional levels were extracted for 1990–2019 from the Global Burden of Disease 2019. Joinpoint regression was used to analyze trends in the ASMR and ASDR of TBL cancer burden related to OCs, and the annual percent change and the average annual percent change (AAPC) were recorded.

Results

The mortality from TBL cancer related to exposure to OCs increased globally. The ASMR and ASDR decreased in both sexes and in men between 1990 and 2019. The AAPC of ASMR and ASDR decreased in men between 1990 and 2019, but increased in women. Asbestos accounted for the highest death number and beryllium accounted for the lowest; diesel engine exhaust caused the largest percentage change in death number (145.3%), in ASDR (14.9%), and in all ages DALY rates (57.6%). Asbestos accounted for the largest death number in high social development index (SDI) countries, whereas low-middle SDI countries had the largest percent change (321.4%). Asbestos was associated with decreased ASDR in high SDI countries and increased ASDR in low-middle SDI countries, and similar changes were observed for other OCs.

Conclusions

The overall mortality and DALYs of TBL cancer burden related to OCs showed a decreasing trend between 1990 and 2019, whereas death number increased. Asbestos accounted for the highest death number. TBL cancer burden related to OCs decreased to different degrees in high, low, low-middle, and middle SDI countries, which showed variable levels of TBL cancer burden related to exposure to OCs (except asbestos).
Intensity Analysis to Study the Dynamics of Reforestation in the Rio Doce Water Basin, Brazil
Authors: Bilintoh Thomas Mumuni
Published: June 2022

Conservationists routinely require methods to analyze the effects of conservation interventions such as reforestation. Although methods to measure the effects of reforestation abound, these methods often fail to track and communicate the complexities of reforestation interventions. This paper uses intensity analysis to analyze the effects of reforestation intervention in Brazil’s Rio Doce water basin. In particular, this paper uses intensity analysis to study the effects of reforestation on three land cover categories in Brazil’s Rio Doce water basin. The illustrative approach analyzes the temporal difference of forest in three land cover maps at time points 1998, 2008, and 2015 from the Rio Doce water basin, Brazil, where each map has four land cover categories: forest formation, pasture, mosaic of agriculture and pasture, and urban infrastructure. Interval level intensity indicates that change is faster during the second time interval (1998–2008). The results from the categorical level intensity show that the forest formation and the mosaic of agriculture and pasture are active gainers during both time intervals, while pasture is an active loser. Forest formation targets the mosaic of agriculture and pasture more intensively at the interval level during the first time interval. Forest formation gains the most from a mosaic of agriculture and pasture while at the same time targeting the mosaic of agriculture and pasture during the second time interval. The mosaic of agriculture and pasture gains the most from both forest formation and pasture during both time intervals but targets pasture. The results for the second time interval show that the reforestation process in the Rio Doce water basin, Brazil, only forced the farmers to move to other forest covers in the vicinity. This process constitutes exchange, which accounts for more than half of the change during the second time interval, and thus contributes 0 to the net change of forest.
How Plants and Bacteria can Clean the Earth
Authors: Ordukhani Leila , Firrincieli Andrea , Freeman John L. , Doty Sharon L.
Published: June 2022

Have you ever seen black smog come out of cars? Or noticed an unnatural oily film on water puddles in parking lots? Humans have polluted practically every corner of our planet and most of it is invisible to our eyes. Sadly, Earth suffers the consequences of this pollution. But scientists have recently found a simple way to restore contaminated soils back to health. In this article, we will talk about how plants and bacteria team up to restore our polluted world!
Strategic Self-Talk Assists Basketball Free Throw Performance Under Conditions of Physical Exertion
Authors: Galanis Evangelos , Hatzigeorgiadis Antonis , Charachousi Fedra , Latinjak Alexander T. , Comoutos Nikos , Theodorakis Yannis
Published: June 2022

The purpose of the present study was to examine the effects of a strategic self-talk intervention on basketball free throw performance under conditions of physical exertion. Forty-one male basketball players (Mage = 23.19 years) participated in the study. Following a baseline assessment, a 3-week intervention was implemented. During this period participants of the two groups practiced the same number of free throws in their training sessions; participants of the experimental group practiced using self-talk and developed personal free throw self-talk plans. In the final assessment, the participants repeated the free throw test following a typical shuttle run task causing increased physical exertion. The results showed that in the final assessment the self-talk group performed significantly better than the control group. Overall, the findings indicate that self-talk can be an effective strategy for basketball players when performing free throw under conditions of physical exertion, which is a typical situation in basketball games.
SENP1 Aberrance and Its Linkage to Clinical Features, Adjuvant Regimen, and Prognosis in Patients With Surgical Non-small Cell Lung Cancer Receiving Adjuvant Chemotherapy
Authors: Yang Qian , Yang Mengmeng , Zhang Jun , Ma Yuquan
Published: June 2022

Background

Small ubiquitin-like modifier-specific protease 1 (SENP1) plays vital roles in cancer progression and chemoresistance, but its prognostic value in non-small cell lung cancer (NSCLC) is vague. This study aimed to explore the correlation of SENP1 with clinical features, adjuvant chemotherapy regimen, and prognosis in patients with surgical NSCLC receiving adjuvant chemotherapy.

Methods

Tumor and adjacent tissues were collected from 157 patients with surgical NSCLC receiving adjuvant chemotherapy. Meanwhile, tumor tissue and paired adjacent tissue specimens were obtained to evaluate SENP1 protein expression by immunohistochemistry (IHC) assay; among which, 102 pairs were used to detect SENP1 messenger RNA (mRNA) by reverse transcription quantitative PCR.

Results

SENP1 IHC score and SENP1 mRNA expression were increased in tumor tissue than adjacent tissue (p < 0.001). Besides, elevated SENP1 IHC score was correlated with > 5 cm tumor size (p = 0.045), lymph node metastasis occurrence (p = 0.003), and advanced tumor-node-metastasis (TNM) stage (p = 0.012); meanwhile, increased SENP1 mRNA expression was associated with histopathological subtype (p = 0.011), lymph node metastasis occurrence (p = 0.008), and higher TNM stage (p = 0.015). Besides, no correlation was found in SENP1 IHC score (p = 0.424) or mRNA expression (p = 0.927) with specific adjuvant chemotherapy regimen. Additionally, both the SENP1 protein (high) (p = 0.003) and mRNA high (p = 0.028) were correlated with poor disease-free survival (DFS), while SENP1 protein high was also associated with shorter overall survival (OS) (p = 0.029). Furthermore, SENP1 protein (high vs. low) was independently associated with unsatisfying DFS [p = 0.009, hazard ratio (HR) = 1.798] and OS (p = 0.049, HR = 1.735).

Conclusion

SENP1 may serve as a potential biomarker to improve the management of patients with surgical NSCLC receiving adjuvant chemotherapy.
Learning Curve Analysis of Microvascular Hepatic Artery Anastomosis for Pediatric Living Donor Liver Transplantation: Initial Experience at A Single Institution
Authors: Zhou Wanyi , Dai Xiaoke , Le Ying , Xing Huiwu , Tan Bingqian , Zhang Mingman
Published: June 2022

Background

The incidence of hepatic artery thrombosis in pediatric living donor liver transplantation (LDLT) is significantly higher than that in adults, and is closely related to the surgeon’s experience with hepatic artery anastomosis. However, there are few studies on the learning curve of hepatic artery anastomosis among surgeons.

Methods

We collected data related to 75 patients who underwent pediatric LDLT and hepatic artery anastomosis independently by the same surgeon. Cumulative sum method (CUSUM) was used to analyse the duration of hepatic artery anastomosis and determine the cut-off value. Patients were divided into two phases according to CUSUM. We analysed the intraoperative and postoperative data and survival outcomes of the included patients.

Results

Total anastomosis duration decreased with an increased number of completed procedures, and the average duration was 42.4 ± 2.20 min. A cut-off value and two phases were identified: 1–43 cases and 44–75 cases. Intraoperative blood loss was significantly lower in phase 2 than in phase 1. The immediate functional changes of total bilirubin (TBIL) and direct bilirubin (DBIL) were significantly also lower in phase 2 than in phase 1. Other functional outcomes, postoperative complications, and the long-term survival rate were not significantly different between the two phases.

Conclusions

Technical competence in pediatric LDLT hepatic artery anastomosis may be achieved after completing 43 cases. It is a safe procedure with a surgical loupe that can be systematized and adopted by pediatric surgeons with sufficient experience via a relatively long learning curve.
Seasonal Patterns of Oestrus and Reproduction in Street Dogs of Indian Cities
Authors: Brill George , Kartal Tamara , Yadav Dev Prakash , Bhyan Mukesh , Patel Chirag , Sheoran Shiv Kumar , Patel Piyush , Painuly Bimmy , Chaudhari Amit
Published: June 2022

Understanding seasonal breeding dynamics is essential for maximizing efficiency and welfare in the application of street dog management programs. Humane Society International (HSI) has conducted many animal birth control programmes concerning the street dog populations in urban India. This paper analyses the data on reproductive indicators—oestrus, pregnancy, and pups—collected by HSI sterilization clinics in the cities of Jamshedpur, Dehradun, and Vadodara over a period of 3, 5, and 4 years, respectively. We found a consistent reproductive seasonality dynamic in all three cities, with peak oestrus and pregnancies occurring in the late/post-monsoon season. Pup proportions peaked soon after. Both these findings are consistent with previous studies of free-roaming domestic dog populations both in India and worldwide. Additionally, we identified minor inter-city differences in the temporal breeding dynamic, which we propose are the result of localized seasonal climatic and human factors. Finally, we examine and assert the relevance of breeding seasonality in the implementation of efficient and welfare-sensitive birth control programmes.
Case Report: Hindlimb Ataxia Concurrent With Seizures by Presumed Osmotic Demyelination Syndrome in a Dog
Authors: Lee Ga-Won , Kang Min-Hee , Park Hee-Myung
Published: June 2022

A 6-year-old castrated male Chihuahua dog was presented with hindlimb paresis and ataxia. The dog had hyponatremia and was diagnosed as hypoadrenocorticism 10 days before its visit, and the neurologic signs including generalized tonic seizures and hindlimb paresis occurred 3 days after correction of hyponatremia at a referral hospital. Based on history and clinical findings, osmotic demyelination syndrome (ODS) secondary to rapid correction of hyponatremia was highly suspected. After administration of anti-convulsant and supplements, seizures did not occur, and gait was normalized within 2 weeks. Phenobarbital was tapered and finally discontinued after 3 months, and seizure did not recur. The neurologic signs were completely resolved and the dog continued to be free of neurologic or additional clinical signs over the 19-month follow-up period. ODS should be included among the differential diagnoses in case of any acute neurological dysfunction that occurs with episodes of rapid correction of hyponatremia. To the author's knowledge, this is the rare case report of a dog with hypoadrenocorticism and presumed ODS after rapid correction of hyponatremia leading to neurologic signs including seizures and ataxia.
Canine-Assisted Interventions and the Relevance of Welfare Assessments for Human Health, and Transmission of Zoonosis: A Literature Review
Authors: Meers Lieve Lucia , Contalbrigo Laura , Samuels William Ellery , Duarte-Gan Carolina , Berckmans Daniel , Laufer Stephan Jens , Stevens Vicky Antoinette , Walsh Elizabeth Ann , Normando Simona
Published: June 2022

CAIs (canine-assisted interventions) include “canine-assisted therapy” in which a therapist sets client-oriented goals, 'canine-assisted activities' with recreational goals for clients, and 'canine-assisted education/learning' in which teachers or coaches create learning goals for students or clients. CAIs vary in nearly every way; their only common trait is the involvement of dogs to respond to human need. However, the benefits of involving dogs are highly dependent on the animal's health and behavior. A dog exhibiting negative behavior or an unwell dog might pose a risk, especially for CAI target groups, specifically individuals with immunosuppression, chronic illness, children, elderly, etc. Therefore, positive animal welfare as preventative medicine to avoid incidents or transmission of zoonosis is an attractive hypothesis, with implications for human and animal, health and well-being. This review aims to summarize the current published knowledge regarding different aspects of welfare in CAIs and to discuss their relevance in the light of health and safety in CAI participants. As method for this study, a literature search was conducted (2001–2022) using the Prisma method, describing issues of dog welfare as defined in the Welfare Quality® approach. This welfare assessment tool includes 4 categories related to behavior, health, management, and environment; it was, therefore, applicable to CAIs. Results indicate that dogs working in CAIs are required to cope with diverse variables that can jeopardize their welfare. In conclusion, we propose regular welfare assessments for dogs in CAIs, which would also protect the quality of the CAI sessions and the clients' safety and well-being.
Effects of Nitrogen Fertilization on Physiological Response of Maize to Soil Salinity
Authors: Du Kaixuan , Zhang Yanqun , Qin Shanshan , Wang Lijuan , Zhang Baozhong , Wang Shuji
Published: June 2022

Soil salinization is a global problem that causes huge losses in agricultural production. Salt can interfere with crop absorption and metabolism of nutrients and water, affect plant physiological responses and reduce plant biomass. Maize, a very important economic crop, can adapt to a certain degree of saline-alkali soil. It is essential to understand the physiological indexes of response to soil salinity concentrations and explore the effects of different nitrogen fertilizer treatments on maize growth. In this study, three soil salinity gradients (S1, S2 and S3 were with soil salt concentration, Ssc, of 0, 0.1% and 0.25%, respectively) and two nitrogen application rates (N0 and N1 were without and with nitrogen applied (13.2 g per pot), respectively) were set up. Plant growth and photosynthetic parameters were measured. Whether nitrogen was applied or not, with the increase in Ssc, leaf area, plant height, stem diameter, SPAD, leaf water potential, RuBP carboxylase, and PEP carboxylase activities, photosynthetic rate (A), stomatal conductance (gs), the maximum stomatal conductance (gsmax), and the stomatal morphological parameters such as stomatal width and maximum stomatal area (amax), all showed a downward trend. Under the S1 and S2 treatments, compared with the N0, the N1 treatment alleviated the stress effect of the Ssc on these indicators. However, under S3 treatment, the stress degrees of leaf water potential, gs, gsmax and amax, were aggravated after nitrogen application. This indicated that under the high Ssc of S3, the interaction between nitrogen application and soil salinity should be considered. WUEin increased with the increase in Ssc. Moreover, under N1 treatments, the increase in WUEin with Ssc was greater than that with N0. With the increase in Ssc, whether nitrogen was applied or not, the dry weight of maize declined by 44.2% and 73.0%, respectively, for the S2 and S3 treatments. Under S2 treatment, N1 significantly improved the dry matter mass of maize compared with the N0 treatment. The results showed that soil salt stress can inhibit crop growth, physiology and dry matter accumulation, and that nitrogen application can alleviate this within a specific salinity range. Such results indicate that in saline-alkali areas, whether nitrogen fertilizer is applied or not should depend on the level of Ssc to improve plant growth.