In vitro Evaluation of the Synergistic Anti-inflammatory Activities of the Combined Hydroalcoholic Extracts from Mentha Pulegium L. and Pimpinella Anisum L.
Authors: Zatla Amina Tabet , Hammoudi Amina , El Amine DIB Mohammed , Perard Joëlle 
Published: February 2025

Background:

Chronic inflammatory diseases are rising, driving the search for effective natural treatments. Mentha pulegium L. and Pimpinella anisum L. (anise) exhibit notable anti-inflammatory properties individually, but their combined effects are less stud-ied. This research evaluates the in vitro synergistic anti-inflammatory activities of their hydroalcoholic extracts. Phytochemical analysis confirmed the presence of flavonoids, tannins, and polyphenols in both extracts. Individually, they demonstrated significant ac-tivity (78.5% and 72.3%, respectively, at 10 g/L) compared to Diclofenac (62.3%). Their combination achieved 88.6% inhibition at the same concentration. These findings high-light their potential as natural anti-inflammatory agents.

Introduction:

The rise in chronic inflammatory diseases has increased interest in natural anti-inflammatory treatments. Mentha pulegium L. and Pimpinella anisum L. are well-known for their anti-inflammatory potential, attributed to their bioactive compounds like flavonoids and polyphenols. While the individual effects of these plants are established, their combined use is underexplored. This study evaluates the in vitro synergistic anti-inflammatory activity of hydroalcoholic extracts from these plants, aiming to offer an ef-fective natural therapeutic alternative.

Methods:

Hydroalcoholic extracts were prepared by maceration of both plants. Anti-in-flammatory activity was assessed using the protein denaturation method with Diclofenac as a standard.

Results:

Phytochemical analysis identified flavonoids, alkaloids, tannins, glycosides, and polyphenols in Mentha pulegium, with an absence of saponins. Pimpinella anisum con-tained flavonoids, tannins, heterosides, and polyphenols. The extracts exhibited strong anti-inflammatory activity individually (78.5% and 72.3%, respectively, at 10 g/L) and even higher inhibition (88.6%) when combined, surpassing Diclofenac (62.3%).

result:

The phytochemical analysis of the hydroalcoholic extract of Mentha pulegium L. revealed a notable presence of flavonoids, alkaloids, tannins, glycosides, and polyphenols but the absence of saponins, In the hydroalcoholic extract of Pimpinella anisum L., significant quantities of flavonoids, tannins, heterosides, and polyphenols were identified. The results showed that the hydroalcoholic extract of Mentha pulegium L. and Pimpinella anisum L. has a very good effect, with percentages of 78.5% and 72.3%, respectively, at a concentration of 10 g/L compared to Diclofenac (62.3%). However, the combination of this two hydroalcoholic extracts demonstrated a high inhibition (88.6%) at the same concentration (10 g/L).

Conclusion:

The combination of Mentha pulegium and Pimpinella anisum extracts sig-nificantly enhanced anti-inflammatory activity compared to individual extracts, under-scoring their potential as natural therapeutic alternatives to conventional treatments.