bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploration and Characterization of Dynamic Properties for Cultural Heritage Conservation: A Case Study for Historical Stone Masonry Buildings in Zanzibar
Authors: Ali Bimkubwa Seif , Castro Juan Jose , Omi Shogo , Nazimi Karishma
Published: April 2024

Ancient civilizations have imprinted their legacy on Zanzibar Stone Town through the construction of revered stone masonry buildings, which are experiencing rapid deterioration due to severe ambient environmental impacts. In response to these challenges, this study presents a comprehensive field exploration through the ambient vibration test (AVT) and numerical prediction of historical stone masonry buildings in Zanzibar Stone Town to analyze the dynamic characteristics. The ambient vibration test (AVT) reveals structural dynamic properties in terms of peak resonance frequencies (fr(avg)), mode shape, and damping ratio, in conjunction with the development of correlation with the geometric parameters of the building. The results reveal fr(avg) ranges between 2.8 and 5.3 Hz for investigated structures, non-uniform deformed mode shapes, and damping ratio ranges between 1.35% and 4.45% at various orientation axes of the understudy buildings. However, the relationship between natural frequencies indicates a higher association with the geometrical parameters of the building, yielding a correlation coefficient (R2) between 0.85 and 0.99. Moreover, the numerical prediction via eigenvalue analysis (EVA) yields a considerable association with the investigated data, quantified by root mean square error (RMSE), mean absolute error (MAE) ranged between 0.29 and 0.3, with Nash–Sutcliffe efficiency (NSE) and R2 between 0.81 and 0.99, respectively. Furthermore, conservation work guidelines were also developed to assist the structural engineer and conservationist in adopting targeted conservation strategies for the efficient preservation of the historical integrity in Stone Town.
Strategies for Mitigating Urban Residential Carbon Emissions: A System Dynamics Analysis of Kunming, China
Authors: Xu Jian , Qian Yujia , He Bingyue , Xiang Huixuan , Ling Ran , Xu Genyu
Published: April 2024

To effectively combat environmental challenges, it is necessary to evaluate urban residential building carbon emissions and implement energy-efficient, emission-reducing strategies. The lack of a specialized carbon emission monitoring system complicates merging macro- and micro-level analyses to forecast urban residential emissions accurately. This study employs a system dynamics (SD) model to examine the influence of social, economic, energy, and environmental factors on carbon emissions in urban residences in Kunming, China. The SD model forecasts household carbon emissions from 2022 to 2030 and establishes three scenarios: a low-carbon scenario (LCS), a medium low-carbon scenario (MLCS), and a high low-carbon scenario (HLCS) to assess emission reduction potentials. It predicts emissions will climb to 4.108 million tons by 2030, significantly surpassing the 2014 baseline, with economic growth, urbanization, residential energy consumption, and housing investment as key drivers. To curb emissions, the study suggests enhancing low-carbon awareness, altering energy sources, promoting research and development investment, and expanding green areas. The scenarios indicate a 5.1% to 16.1% emission reduction by 2030 compared to the baseline. The study recommends an 8.3% to 11.4% reduction in MLCS as a practical short-term target for managing urban residential emissions, offering a valuable SD approach for optimizing carbon strategies and aiding low-carbon development.
Component Identification and Depth Estimation for Structural Images Based on Multi-Scale Task Interaction Network
Authors: Ye Jianlong , Yu Hongchuan , Liu Gaoyang , Zhou Jiong , Shu Jiangpeng
Published: April 2024

Component identification and depth estimation are important for detecting the integrity of post-disaster structures. However, traditional manual methods might be time-consuming, labor-intensive, and influenced by subjective judgments of inspectors. Deep-learning-based image visual inspection is a new approach to overcome these problems, but repeated modeling is required for different inspection tasks, which limits inspection accuracy and practical deployment efficiency. In this study, it is observed that the matched ratios of pixel pairs between component identification and depth estimation reach a high value, which indicates the dual tasks are highly related. Therefore, the Multi-Scale Task Interaction Network (MTI-Net) is proposed for structural images to simultaneously accomplish both tasks for accurate and efficient structural inspection. It propagates distilled task information from lower to higher scales. Then, it aggregates the refined task features from all scales to produce the final per-task predictions. Experiments show that MTI-Net delivers the full potential of multi-task learning, with a smaller memory footprint and higher efficiency compared to single-task learning. For the evaluation metrics of model performance, the mean Intersection over Union (mIoU) of component identification improves by 2.30, and root mean square error (RMSE) drops by 0.36 m with the aid of the multi-task strategy. The multi-task deep learning framework has great potential value in engineering applications.
Contemporary Evaporative Cooling System with Indirect Interaction in Construction Implementations: A Theoretical Exploration
Authors: Cuce Pinar Mert , Cuce Erdem , Riffat Saffa
Published: April 2024

The construction sector, including in developed countries, plays a notable part in the overall energy consumption worldwide, being responsible for 40% of it. In addition to this, heating, ventilating and air-conditioning (HVAC) systems constitute the largest share in this sector, accounting for 40% of energy usage in construction and 16% globally. To address this, stringent rules and performance measures are essential to reduce energy consumption. This study focuses on mathematical optimisation modelling to enhance the performance of indirect-contact evaporative cooling systems (ICESs), a topic with a significant gap in the literature. This modelling is highly comprehensive, covering various aspects: (1) analysing the impact of the water-spraying unit (WSU) size, working air (WA) velocity and hydraulic diameter (Dh) on the evaporated water vapour (EWV) amount; (2) evaluating temperature and humidity distribution for a range of temperatures without considering humidity at the outlet of the WSU, (3) presenting theoretical calculations of outdoor temperature (Tout) and humidity with a constant WSU size and air mass flow rate (MFR), (4) examining the combined effect of the WA MFR and relative humidity (ϕ) on Tout and (5) investigating how Tout influences the indoor environment’s humidity. The study incorporates an extensive optimisation analysis. The findings indicate that the model could contribute to the development of future low-carbon houses, considering factors such as the impact of Tout on indoor ϕ, the importance of low air velocity for achieving a low air temperature, the positive effects of Dh on outdoor air and the necessity of a WSU with a size of at least 8 m for adiabatic saturation.
Obesity and Inflammatory Factors in the Progression of Early-Onset Colorectal Cancer
Authors: Jones Alexandra N. , Scheurlen Katharina M. , Macleod Anne , Simon Hillary L. , Galandiuk Susan
Published: April 2024

Metabolic dysfunction associated with obesity leads to a chronic pro-inflammatory state with systemic effects, including the alteration of macrophage metabolism. Tumor-associated macrophages have been linked to the formation of cancer through the production of metabolites such as itaconate. Itaconate downregulates peroxisome proliferator-activated receptor gamma as a tumor-suppressing factor and upregulates anti-inflammatory cytokines in M2-like macrophages. Similarly, leptin and adiponectin also influence macrophage cytokine expression and contribute to the progression of colorectal cancer via changes in gene expression within the PI3K/AKT pathway. This pathway influences cell proliferation, differentiation, and tumorigenesis. This work provides a review of obesity-related hormones and inflammatory mechanisms leading to the development and progression of early-onset colorectal cancer (EOCRC). A literature search was performed using the PubMed and Cochrane databases to identify studies related to obesity and EOCRC, with keywords including ‘EOCRC’, ‘obesity’, ‘obesity-related hormones’, ‘itaconate’, ‘adiponectin’, ‘leptin’, ‘M2a macrophage’, and ‘microbiome’. With this concept of pro-inflammatory markers contributing to EOCRC, increased use of chemo-preventative agents such as aspirin may have a protective effect. Elucidating this association between obesity-related, hormone/cytokine-driven inflammatory effects with EOCRC may help lead to new therapeutic targets in preventing and treating EOCRC.
Transperineal Laser Ablation (TPLA) Treatment of Focal Low–Intermediate Risk Prostate Cancer
Authors: Manenti Gugliemo , Perretta Tommaso , Nezzo Marco , Fraioli Federico Romeo , Carreri Beatrice , Gigliotti Paola Elda , Micillo Andrea , Malizia Andrea , Di Giovanni Daniele , Ryan Colleen Patricia , Garaci Francesco Giuseppe
Published: April 2024

Background: This interventional pilot study aimed to evaluate the short-term (3 years) efficacy of focal laser ablation (FLA) in treating the index lesion of low–intermediate-risk prostate cancer, along with assessing the safety of the procedure (ClinicalTrials.gov ID NCT04045756). Methods: Forty patients aged between 46 and 86 with histologically proven organ-confined prostate cancer and low-to-intermediate progression risk were included. FLA was performed under percutaneous fusion magnetic resonance/ultrasound guidance in a Day Hospital setting under local anesthesia. Patients underwent regular clinical and functional assessments through the international index of erectile function (IIEF-5) and the International Prostatism Symptom Score (IPSS), PSA measurements, post-procedure MRI scans, and biopsies at 36 months or if positive findings were detected earlier. Statistical analyses were conducted to assess trends in PSA levels and cavity dimensions over time. Results: Forty patients were initially included, with fifteen lost to follow-up. At 36 months, a mean PSA reduction of 60% was observed, and 80% of MRI scans showed no signs of in-field clinically significant residual/recurrent cancer. Biopsies at 36 months revealed no malignant findings in 20 patients. No deterioration in sexual function or urinary symptoms was recorded. Conclusions: FLA appears to be safe, feasible, and effective in the index lesion treatment of low–intermediate-risk prostate cancer, with a high rate of tumor eradication and preservation of quality of life.
Clinical and Analytical Validation of Two Methods for Ki-67 Scoring in Formalin Fixed and Paraffin Embedded Tissue Sections of Early Breast Cancer
Authors: Đokić Snežana , Gazić Barbara , Grčar Kuzmanov Biljana , Blazina Jerca , Miceska Simona , Čugura Tanja , Grašič Kuhar Cvetka , Jeruc Jera
Published: April 2024

Proliferation determined by Ki-67 immunohistochemistry has been proposed as a useful prognostic and predictive marker in breast cancer. However, the clinical validity of Ki-67 is questionable. In this study, Ki-67 was retrospectively evaluated by three pathologists using two methods: a visual assessment of the entire slide and a quantitative assessment of the tumour margin in 411 early-stage breast cancer patients with a median follow-up of 26.8 years. We found excellent agreement between the three pathologists for both methods. The risk of recurrence for Ki-67 was time-dependent, as the high proliferation group (Ki-67 ≥ 30%) had a higher risk of recurrence initially, but after 4.5 years the risk was higher in the low proliferation group. In estrogen receptor (ER)-positive patients, the intermediate Ki-67 group initially followed the high Ki-67 group, but eventually followed the low Ki-67 group. ER-positive pN0-1 patients with intermediate Ki-67 treated with endocrine therapy alone had a similar outcome to patients treated with chemotherapy. A cut-off value of 20% appeared to be most appropriate for distinguishing between the high and low Ki-67 groups. To summarize, a simple visual whole slide Ki-67 assessment turned out to be a reliable method for clinical decision-making in early breast cancer patients. We confirmed Ki-67 as an important prognostic and predictive biomarker.
Progress in Biological Research and Treatment of Pseudomyxoma Peritonei
Authors: Li Xi , Liu Guodong , Wu Wei
Published: April 2024

Pseudomyxoma peritonei (PMP) is a rare disease characterized by extensive peritoneal implantation and mass secretion of mucus after primary mucinous tumors of the appendix or other organ ruptures. Cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) is currently the preferred treatment, with excellent efficacy and safety, and is associated with breakthrough progress in long-term disease control and prolonged survival. However, the high recurrence rate of PMP is the key challenge in its treatment, which limits the clinical application of multiple rounds of CRS-HIPEC and does not benefit from conventional systemic chemotherapy. Therefore, the development of alternative therapies for patients with refractory or relapsing PMP is critical. The literature related to PMP research progress and treatment was searched in the Web of Science, PubMed, and Google Scholar databases, and a literature review was conducted. The overview of the biological research, treatment status, potential therapeutic strategies, current research limitations, and future directions associated with PMP are presented, focuses on CRS-HIPEC therapy and alternative or combination therapy strategies, and emphasizes the clinical transformation prospects of potential therapeutic strategies such as mucolytic agents and targeted therapy. It provides a theoretical reference for the treatment of PMP and the main directions for future research.
Unveiling IL6R and MYC as Targeting Biomarkers in Imatinib-Resistant Chronic Myeloid Leukemia through Advanced Non-Invasive Apoptosis Detection Sensor Version 2 Detection
Authors: Lee Chia-Hwa , Hsu Kai-Wen , Hsieh Yao-Yu , Li Wei-Ting , Long Yuqing , Lin Chun-Yu , Chen Shu-Huey
Published: April 2024

The management of chronic myelogenous leukemia (CML) has seen significant progress with the introduction of tyrosine kinase inhibitors (TKIs), particularly Imatinib. However, a notable proportion of CML patients develop resistance to Imatinib, often due to the persistence of leukemia stem cells and resistance mechanisms independent of BCR::ABL1 This study investigates the roles of IL6R, IL7R, and MYC in Imatinib resistance by employing CRISPR/Cas9 for gene editing and the Non-Invasive Apoptosis Detection Sensor version 2 (NIADS v2) for apoptosis assessment. The results indicate that Imatinib-resistant K562 cells (K562-IR) predominantly express IL6R, IL7R, and MYC, with IL6R and MYC playing crucial roles in cell survival and sensitivity to Imatinib. Conversely, IL7R does not significantly impact cytotoxicity, either alone or in combination with Imatinib. Further genetic editing experiments confirm the protective functions of IL6R and MYC in K562-IR cells, suggesting their potential as therapeutic targets for overcoming Imatinib resistance in CML. This study contributes to understanding the mechanisms of Imatinib resistance in CML, proposing IL6R and MYC as pivotal targets for therapeutic strategies. Moreover, the utilization of NIADS v2 enhances our capability to analyze apoptosis and drug responses, contributing to a deeper understanding of CML pathogenesis and treatment options.
The Role of Semaphorins in the Pathogenesis of Rheumatoid Arthritis
Authors: Rosik Jakub , Kulpa Joanna , Szczepanik Marcin , Pawlik Andrzej
Published: April 2024

Rheumatoid arthritis (RA) is one of the most common autoimmune diseases. Inflammation of the synovial fluid propagates the pathological process of angiogenesis. Semaphorins play a crucial role in the context of endothelial cell function, and their pleiotropic nature has various effects on the further development of RA. This narrative review summarises the various roles of semaphorins in the pathology of RA and whether they could play a role in developing novel RA treatment options.
The Natural Product Secoemestrin C Inhibits Colorectal Cancer Stem Cells via p38–S100A8 Feed-Forward Regulatory Loop
Authors: Zhou Huimin , Chen Minghua , Zhao Cong , Shao Rongguang , Xu Yanni , Zhao Wuli
Published: April 2024

Cancer stem cells (CSCs) are closely associated with tumor initiation, metastasis, chemoresistance, and recurrence, which represent some of the primary obstacles to cancer treatment. Targeting CSCs has become an important therapeutic approach to cancer care. Secoemestrin C (Sec C) is a natural compound with strong anti-tumor activity and low toxicity. Here, we report that Sec C effectively inhibited colorectal CSCs and non-CSCs concurrently, mainly by inhibiting proliferation, self-renewal, metastasis, and drug resistance. Mechanistically, RNA-seq analysis showed that the pro-inflammation pathway of the IL17 axis was enriched, and its effector S100A8 was dramatically decreased in Sec C-treated cells, whose roles in the stemness of CSCs have not been fully clarified. We found that the overexpression of S100A8 hindered the anti-CSCs effect of Sec C, and S100A8 deficiency attenuated the stemness traits of CSCs to enhance the Sec C killing activity on them. Meanwhile, the p38 signal pathway, belonging to the IL17 downstream axis, can also mediate CSCs and counter with Sec C. Notably, we found that S100A8 upregulation increased the p38 protein level, and p38, in turn, promoted S100A8 expression. This indicated that p38 may have a mutual feedback loop with S100A8. Our study discovered that Sec C was a powerful anti-colorectal CSC agent, and that the positive feedback loop of p38–S100A8 mediated Sec C activity. This showed that Sec C could act as a promising clinical candidate in colorectal cancer treatment, and S100A8 could be a prospective drug target.
Comparative Analysis of Donnan Steric Partitioning Pore Model and Dielectric Exclusion Applied to the Fractionation of Aqueous Saline Solutions through Nanofiltration
Authors: Saavedra Aldo , Valdés Hugo , Velásquez Juan , Hernández Sebastián
Published: April 2024

The aim of this study was to analyze, both theoretically and experimentally, the material transport mechanisms governing the separation of ionic species in aqueous solutions using nanofiltration membranes. To interpret the experimental results, the Donnan Steric Partitioning Model (DSPM) and the Dielectric Exclusion Model (DSPM-DE) were applied and computationally simulated in Matlab. Experimental tests were conducted using a pilot-scale system with commercial NF90 membranes. The results indicate that the DSPM better describes the rejection of monovalent ions (sodium and chloride), while the DSPM-DE is more suitable for divalent ions (sulfate and magnesium). Additionally, both models were sensitized to explore the impact of hindrance factors on the rejection of different ionic species. For neutral molecules present in the solution, it was observed that the DSPM and DSPM-DE do not adequately interpret selectivity, suggesting that under such conditions, the electrostatic exclusion mechanism loses significance, with the steric mechanism prevailing.
Nutritional Assessment of Children and Adolescents with Atypical Anorexia Nervosa: A Preliminary Longitudinal Investigation Using the 24-h Dietary Recall
Authors: Valeriani Beatrice , Pruccoli Jacopo , Chiavarino Francesca , Petio Maria Letizia , Parmeggiani Antonia
Published: April 2024

Background: Atypical Anorexia Nervosa (AAN) is a Feeding and Eating Disorder characterized by fear of gaining weight and body image disturbance, in the absence of significantly low body weight. AAN may present specific clinical and psychopathological features. Nonetheless, the literature lacks data concerning the nutritional characteristics and body composition of children and adolescents with AAN and their variation over time. Methods: Case series, including 17 children and adolescents with AAN. All the patients were assessed at the first evaluation (T0) with a standardized dietary assessment (24 h Dietary Recall, 24 hDR). Nutritional data were compared with European dietary reference values (DRVs). Body composition parameters (weight, fat mass, fat-free mass) and their changes over time at two (T1) and six (T2) months were collected as well, using a Bioelectrical impedance analysis (Wunder WBA300 with four poles and foot contact; impedance frequency 50 kHz 500 μA; impedance measurement range 200~1000 Ω/0.1 Ω). Results: The included individuals presented eating behaviors oriented towards significantly low daily energy intake (p < 0.001) compared with DRVs set by the European Food Safety Authority (EFSA) (with low carbohydrates and fats), and increased proteins (p < 0.001). A longer latency before observation (illness duration before observation) correlated with a negative change in weight. Body composition parameters were described, with no significant changes across the six-month outpatient assessment. Discussion: This is the first research to systematically assess the body composition and nutritional features of a group of individuals with AAN in the developmental age. Further research should assess the effect of targeted treatment interventions on body composition and nutritional features.
Enhancing Skills, Mood, and Performance in Overweight Handball Players: Exploring Individual vs. Collective Verbal Encouragement Strategies
Authors: Sahli Faten , Bouzouraa Mohamed Mansour , Rebhi Mahmoud , Romdhani Amir , Sahli Hajer , Salem Atef , Trabelsi Khaled , Ammar Achraf , Zghibi Makram
Published: April 2024

This comparative study investigates the effectiveness of two teaching methods, individual verbal encouragement and collective verbal encouragement, in enhancing the technical–tactical skills and mood state of obese students during handball matches. This study employs a randomized controlled design and involves 28 overweight students (50% females), age: 17.4 ± 2.08 years and BMI: 26.8 ± 1.5 for females and 27.3 ± 2.1 for males. Technical–tactical skills are assessed through performance metrics (individual evaluation proposal by Gréhaigne) such as Ball Played (BP), Conquered Ball (CB), Lost Ball (LB), Shoots/Goals, Conservation index, and defensive index, while mood states are evaluated using pre- and post-tests (BRUMS Scale). Results reveal that individual verbal encouragement significantly enhances technical–tactical skills and positively influences the mood state of overweight students compared to collective verbal encouragement. Boys in Session 1 with VEI displayed a significantly higher number of ball plays (mean difference = 0.94 standard deviations, p = 0.004) and conquered balls (mean difference = 0.78 standard deviations, p = 0.006) compared to VEC. They also had a lower number of Lost Balls (mean difference = −0.62 standard deviations, p = 0.018) and a higher shooting efficiency (Shoots/Goals ratio, mean difference = 0.67 standard deviations; p = 0.013). Similar trends were observed in Session 2, with VEI, again, demonstrating advantages. Girls exhibited analogous improvements with VEI in both sessions. Notably, these performance enhancements coincided with positive emotional changes, with VEI leading to a greater decrease in depression and fatigue scores for both boys and girls. The study highlights the importance of tailoring teaching methods to the specific needs of overweight students in the context of handball, emphasizing the effectiveness of individualized verbal encouragement for skill development and emotional well-being. These findings offer practical implications for educators and coaches involved in physical education, advocating for personalized approaches to optimize learning experiences for overweight students in sports settings.
Calcitriol Treatment Decreases Cell Migration, Viability and β-Catenin Signaling in Oral Dysplasia
Authors: Peña-Oyarzún Daniel , Guzmán Constanza , Kretschmar Catalina , Torres Vicente A. , Maturana-Ramirez Andrea , Aitken Juan , Reyes Montserrat
Published: April 2024

Nearly 90% of oral cancers are characterized as oral squamous cell carcinoma (OSCC), representing the sixth most common type of cancer. OSCC usually evolves from oral potentially malignant disorders that, in some cases, are histologically consistent with a oral dysplasia. The levels of 1α,25 dihydroxyvitamin D3 (1,25-(OH)2D3; calcitriol), the active form of vitamin D3, have been shown to be decreased in patients with oral dysplasia and OSCC. Moreover, treatment with 1,25-(OH)2D3 has been proven beneficial in OSCC by inhibiting the Wnt/β-catenin pathway, a signaling route that promotes cell migration, proliferation, and viability. However, whether this inhibition mechanism occurs in oral dysplasia is unknown. To approach this question, we used dysplastic oral keratinocyte cultures and oral explants (ex vivo model of oral dysplasia) treated with 1,25-(OH)2D3 for 48 h. Following treatment with 1,25-(OH)2D3, both in vitro and ex vivo models of oral dysplasia showed decreased levels of nuclear β-catenin by immunofluorescence (IF) and immunohistochemistry (IHC). Consistently, reduced protein and mRNA levels of the Wnt/β-catenin target gene survivin were observed after treatment with 1,25-(OH)2D3. Moreover, 1,25-(OH)2D3 promoted membranous localization of E-cadherin and nuclear localization of vitamin D receptor (VDR). Functionally, DOK cells treated with 1,25-(OH)2D3 displayed diminished cell migration and viability in vitro.
Biochemical and Molecular Basis of Chemically Induced Defense Activation in Maize against Banded Leaf and Sheath Blight Disease
Authors: Hamidi Shah Mahmood , Meshram Shweta , Kumar Aundy , Singh Archana , Yadav Rajbir , Gogoi Robin
Published: April 2024

Maize is the third most vital global cereal, playing a key role in the world economy and plant genetics research. Despite its leadership in production, maize faces a severe threat from banded leaf and sheath blight, necessitating the urgent development of eco-friendly management strategies. This study aimed to understand the resistance mechanisms against banded leaf and sheath blight (BLSB) in maize hybrid “Vivek QPM-9”. Seven fungicides at recommended doses (1000 and 500 ppm) and two plant defense inducers, salicylic acid (SA) and jasmonic acid (JA) at concentrations of 50 and 100 ppm, were applied. Fungicides, notably Azoxystrobin and Trifloxystrobin + Tebuconazole, demonstrated superior efficacy against BLSB, while Pencycuron showed limited effectiveness. Field-sprayed Azoxystrobin exhibited the lowest BLSB infection, correlating with heightened antioxidant enzyme activity (SOD, CAT, POX, β-1,3-glucanase, PPO, PAL), similar to the Validamycin-treated plants. The expression of defense-related genes after seed priming with SA and JA was assessed via qRT-PCR. Lower SA concentrations down-regulated SOD, PPO, and APX genes but up-regulated CAT and β-1,3-glucanase genes. JA at lower doses up-regulated CAT and APX genes, while higher doses up-regulated PPO and β-1,3-glucanase genes; SOD gene expression was suppressed at both JA doses. This investigation elucidates the effectiveness of certain fungicides and plant defense inducers in mitigating BLSB in maize hybrids and sheds light on the intricate gene expression mechanisms governing defense responses against this pathogen.
Autism Spectrum Disorder- and/or Intellectual Disability-Associated Semaphorin-5A Exploits the Mechanism by Which Dock5 Signalosome Molecules Control Cell Shape
Authors: Okabe Miyu , Sato Takanari , Takahashi Mikito , Honjo Asahi , Okawa Maho , Ishida Miki , Kukimoto-Niino Mutsuko , Shirouzu Mikako , Miyamoto Yuki , Yamauchi Junji
Published: April 2024

Autism spectrum disorder (ASD) is a neurodevelopmental disorder that includes autism, Asperger’s syndrome, and pervasive developmental disorder. Individuals with ASD may exhibit difficulties in social interactions, communication challenges, repetitive behaviors, and restricted interests. While genetic mutations in individuals with ASD can either activate or inactivate the activities of the gene product, impacting neuronal morphogenesis and causing symptoms, the underlying mechanism remains to be fully established. Herein, for the first time, we report that genetically conserved Rac1 guanine-nucleotide exchange factor (GEF) Dock5 signalosome molecules control process elongation in the N1E-115 cell line, a model line capable of achieving neuronal morphological changes. The increased elongation phenotypes observed in ASD and intellectual disability (ID)-associated Semaphorin-5A (Sema5A) Arg676-to-Cys [p.R676C] were also mediated by Dock5 signalosome molecules. Indeed, knockdown of Dock5 using clustered regularly interspaced short palindromic repeat (CRISPR)/CasRx-based guide(g)RNA specifically recovered the mutated Sema5A-induced increase in process elongation in cells. Knockdown of Elmo2, an adaptor molecule of Dock5, also exhibited similar recovery. Comparable results were obtained when transfecting the interaction region of Dock5 with Elmo2. The activation of c-Jun N-terminal kinase (JNK), one of the primary signal transduction molecules underlying process elongation, was ameliorated by either their knockdown or transfection. These results suggest that the Dock5 signalosome comprises abnormal signaling involved in the process elongation induced by ASD- and ID-associated Sema5A. These molecules could be added to the list of potential therapeutic target molecules for abnormal neuronal morphogenesis in ASD at the molecular and cellular levels.
The Property of a Key Amino Acid Determines the Function of Farnesyl Pyrophosphate Synthase in Sporobolomyces pararoseus NGR
Authors: Wang Yunjiao , Zhang Ning , Yan Jianyu , Li Chunwang , Zeng Nan , Wang Dandan , Li Zijing , Li Bingxue , An Yingfeng
Published: April 2024

Farnesyl pyrophosphate synthase (FPPS) catalyzes the synthesis of C15 farnesyl diphosphate (FPP) from C5 dimethylallyl diphosphate (DMAPP) and two or three C5 isopentenyl diphosphates (IPPs). FPP is an important precursor for the synthesis of isoprenoids and is involved in multiple metabolic pathways. Here, farnesyl pyrophosphate synthase from Sporobolomyces pararoseus NGR (SpFPPS) was isolated and expressed by the prokaryotic expression system. The SpFPPS full-length genomic DNA and cDNA are 1566 bp and 1053 bp, respectively. This gene encodes a 350-amino acid protein with a predicted molecular mass of 40.33 kDa and a molecular weight of 58.03 kDa (40.33 kDa + 17.7 kDa), as detected by SDS-PAGE. The function of SpFPPS was identified by induction, purification, protein concentration and in vitro enzymatic activity experiments. Structural analysis showed that Y90 was essential for chain termination and changing the substrate scope. Site-directed mutation of Y90 to the smaller side-chain amino acids alanine (A) and lysine (K) showed in vitro that wt-SpFPPS catalyzed the condensation of the substrate DMAPP or geranyl diphosphate (GPP) with IPP at apparent saturation to synthesize FPP as the sole product and that the mutant protein SpFPPS-Y90A synthesized FPP and C20 geranylgeranyl diphosphate (GGPP), while SpFPPS-Y90K hydrolyzed the substrate GGPP. Our results showed that FPPS in S. pararoseus encodes the SpFPPS protein and that the amino acid substitution at Y90 changed the distribution of SpFPPS-catalyzed products. This provides a baseline for potentially regulating SpFPPS downstream products and improving the carotenoid biosynthesis pathway.
Wear Behavior Assessment of New Wire-Arc Additively Manufactured Surfaces on AA6061 and AA5086 Alloys through Multi-Walled Carbon Nanotubes and Ni Particles Inducement
Authors: Muzamil Muhammad , Iqbal Syed Amir , Anwar Muhammad Naveed , Samiuddin Muhammad , Yang Junzhou , Raza Muhammad Ahmed
Published: April 2024

This study investigates the new surface development on AA6061 and AA5086 alloys considering the wire-arc additive manufacturing technique as a direct energy deposition (DED) process of wire. Two different quantities of MWCNTs, i.e., 0.01 (low) and 0.02 (high) g, with a constant nickel (Ni) weight (0.2 g) were pre-placed in the created square patterns. ER4043 filler was used as a wire for additive deposition, and an arc was generated through a tungsten inert gas (TIG) welding source. Furthermore, hardness and pin-on-disk wear-testing methods were employed to measure the changes at the surfaces with the abovementioned inducements. This work was designed to illustrate the hardness and the offered wear resistance in terms of mass loss of the AA6061 and AA5086 aluminum alloys with the function of nano-inducements. Two sliding distance values of 500 m and 600 m were selected for the wear analysis of mass loss from tracks. A maximum increase in hardness for AA6061 and AA5086 alloys was observed in the experiments, with average values of 70.76 HRB and 74.86 HRB, respectively, at a high mass content of MWCNTs. Moreover, the tribological performance of the modified surfaces improved with the addition of MWCNTs with Ni particles in a broader sense; the modified surfaces performed exceptionally well for AA5086 compared to AA6061 with 0.02 and 0.01 g additions, respectively. The system reported a maximum of 38.46% improvement in mass loss for the AA5086 alloy with 0.02 g of MWCNTs. Moreover, the morphological analysis of the developed wear tracks and the mechanism involved was carried out using scanning electron microscope (SEM) images.
Bioinspired Living Coating System for Wood Protection: Exploring Fungal Species on Wood Surfaces Coated with Biofinish during Its Service Life
Authors: Poohphajai Faksawat , Gubenšek Ana , Černoša Anja , Butina Ogorelec Karen , Rautkari Lauri , Sandak Jakub , Sandak Anna
Published: April 2024

Biofinish is an innovative wood protection system inspired by biological processes. It enhances the hydrophobicity of wood through oil treatment, resulting in improved dimensional stability. Living cells of the fungus Aureobasidium pullulans effectively protect wood from deterioration caused by other decaying fungi. The melanin pigment produced by the fungus provides an appealing dark surface and additionally protects the wood substrate against UV radiation. The significant advantage of Biofinish is its remarkable self-healing ability, which distinguishes it from conventional wood protection methods. This research aimed to explore fungal species colonising surfaces exposed to natural weathering and assess the survival of A. pullulans on wood surfaces coated with Biofinish during its in-service period. This study was performed on a facade composed of European larch wood (Larix decidua) treated with linseed oil and coated with Biofinish at the InnoRenew CoE building in Izola, Slovenia, following a 9-month exposure period. The majority of the detected species belonged to the genera Aureobasidium. The results indicated the survival and effective antagonistic action of A. pullulans, the living and active ingredient of the coating, against other wood-decaying fungi.
Unlocking the Potential: A Comprehensive Analysis of the Technological Properties and Consumer Perception of Shampoo Enriched with Patchouli Extract and Allantoin
Authors: Žlabienė Ugnė , Bartkutė Erlita , Bernatonienė Jurga
Published: April 2024

Amidst a growing shift towards eco-friendly choices in personal care products, the challenge of formulating herbal shampoos with efficacy comparable to synthetic counterparts persists. This study investigates the potential of incorporating patchouli extract and allantoin as additives in anti-dandruff shampoo formulations, assessing their impact on the technological properties of the product. With limited research on their efficacy, our investigation contributes valuable insights to the development of effective and consumer-friendly shampoos targeting dandruff concerns. Physicochemical characteristics (pH, surface tension, texture) were evaluated, alongside specific quality assessments such as wetting time, dirt dispersion, foaming, and cleaning action, in in vivo consumer research. Shampoo formulations incorporating 0.5% Patchoul’Up™ and 1% allantoin exhibited acceptable properties. However, the addition of plant-derived ingredients resulted in a beneficial decrease in surface tension (5.87%). Nevertheless, a decrease in cohesiveness (18%) over a 5-month period resulted in rheological changes, indicating potential instability (p < 0.05). While the consumer evaluation aligns with laboratory findings, continuous research is essential to ensure stability and validate the anti-dandruff potential of the formulation, both in vitro and in vivo. This involves expanding the number of volunteers, with a specific focus on individuals experiencing dandruff concerns, to assess the shampoo’s efficacy and impact on diverse user experiences.
Development of Algorithm for Determining N Fertiliser Requirements of Winter Wheat Based on N Status Using APSIM Modelling
Authors: Vogeler Iris , Kumar Uttam , Knudsen Leif , Hansen Elly M. , Snow Val , Thomsen Ingrid K.
Published: April 2024

The determination of optimum nitrogen (N) fertilisation rates, which maximise yields and minimise N losses, remains problematic due to unknown upcoming crop requirements and near-future supply by the soil. Remote sensing can be used for determining the crop N status and to assess the spatial variability within a field or between fields. This can be used to improve N fertilisation, provided that the optimal fertilisation rate at the time of fertiliser application for an expected yield is known. Using the APSIM-wheat model, we developed an algorithm that relates the N status of the plants at early development stages to the yield response to N. Simulations were performed for winter wheat under growth conditions in Denmark. To obtain a range of different N status in the biomass at early growth stages, the soil N in autumn was varied from 20 to 180 kg N ha−1, and at BBCH23, fertiliser was applied at a rate of 50 kg N ha−1. In a full factorial setup, additional N fertiliser was applied ranging from 0 to 150 kg N ha−1 during three different development stages (BBCH30, 32, and 37). The algorithm was evaluated by comparing model outputs with a standard N application of 50 kg N ha−1 at BBCH23 and 150 kg N ha−1 at BBCH30. The evaluation showed that, depending on the N status of the soil, the algorithm either provided higher or lower optimal N fertilisation rates when targeting 95% of the maximum yield, and these affected the grain yield and the grain N, as well as the amount of N leaching. Split application of fertiliser into three applications was generally beneficial, with decreased product-related N leaching of up to nearly 30%. Further testing of the model under different environmental conditions is needed before such an algorithm can be used to guide N fertilisation.
On the Energy Dependence of the PL of RE Ions in LuBO3:RE (RE = Ce, Eu, Gd, or Tb)
Authors: Schröder Franziska , Reetz Sven , Jüstel Thomas
Published: April 2024

LuBO3 crystallizes in the calcite type (CaCO3) structure and is a widely applied inorganic host for luminescent materials and scintillators. Even though many scientific works have been published concerning the optical properties of rare-earth-doped LuBO3, so far, no study of the emission spectra as function of the excitation energy of such orthoborates has been conducted. Therefore, this work elaborates on the photoluminescence of RE-doped LuBO3 with RE = Ce3+, Eu3+, Gd3+, or Tb3+, while an emphasize is laid on the energy dependence of these four luminescent compounds. The reflection, emission, and excitation spectra were additionally recorded, and the particle size distribution was determined. Calcite type LuBO3 was successfully obtained, and the characteristic RE luminescence was observed. Furthermore, the Gd3+-doped sample showed no scintillation due to its monovalence and a sensitizer is required to transfer the respective energy to the Gd3+-activator.
Structural Analysis of 3,5-Bistrifluoromethylhydrocinnamic Acid
Authors: Lipiński Piotr F. J. , Łyczko Krzysztof
Published: April 2024

The crystal structure of 3,5-bistrifluoromethylhydrocinnamic acid [systematic name: 3-[3,5-bis(trifluoromethyl)phenyl]propanoic acid], C11H8F6O2, has been determined and described. The structure was subject to the Hirshfeld surface-analysis and CE-B3LYP interaction-energies calculations. The title compound crystallises in the monoclinic P21/c space group with one molecule in the asymmetric unit. The propanoic acid side chain of the studied molecule has a bent conformation. The key supramolecular motif in the crystal structure is a centrosymmetric O–H∙∙∙O hydrogen-bonded dimer (R22(8) in the graph set notation). According to CE-B3LYP, the molecules involved in this motif exhibit the strongest pairwise interaction total energy (Etot = −67.9 kJ/mol). On the other hand, there are seven other interacting molecular pairs with significant Etot values in the range of −17 to −28 kJ/mol. In these, the energy is dominated by the dispersive contribution. A survey of the Cambridge Structural Database revealed that in other 3-phenylpropanoic acid structures, the middle dihedral angle of the propanoic acid side chain is always in the trans conformation. This contrasts the current structure where this dihedral angle is in the gauche conformation. According to the Density Functional Theory calculations in the gas phase (at the B3LYP/aug-cc-pvDZ level), the presence of the two CF3 groups (strong electron-withdrawing character) increases the population of the gauche conformers by a substituent electronic effect, and this may be a minor factor contributing to the appearance of this conformation observed in the solid state.
Pseudo-Polymorphism in 2-Pyridylmethoxy Cone Derivatives of p-tert-butylcalix[4]arene and p-tert-butylhomooxacalix[n]arenes
Authors: Joshi Siddharth , Hickey Neal , Marcos Paula M. , Geremia Silvano
Published: April 2024

This paper investigates pseudo-polymorphism in 2-pyridylmethoxy derivatives of p-tert-butylcalix[4]arene (PyC4), p-tert-butyldihomooxa-calix[4]arenes (PyHOC4), and p-tert-butylhexahomotrioxacalix[3]arenes (PyHO3C3), presenting 11 crystal structures with 15 crystallographically independent molecules. The macrocycle of PyC4 is smaller and less flexible with respect to those of PyHOC4 and PyHO3C3, and in solution, the cone conformation of these three molecules exhibits different point symmetries: C4, Cs, and C3, respectively. A correlation is observed between the macrocycle’s structural rigidity and the number of pseudo-polymorphs formed. The more rigid PyC4 displays a higher number (six) of pseudo-polymorphs compared to PyHOC4 and PyHO3C3, which exhibit a smaller number of crystalline forms (three and two, respectively). The X-ray structures obtained show that the conformation of the macrorings is primarily influenced by the presence of an acetonitrile guest molecule within the cavity, with limited impact from crystal packing and intermolecular co-crystallized solvent molecules. Notably, both calix[4]arene derivatives produce a host–guest complex with acetonitrile, while the most flexible and less aromatic PyHO3C3 does not give crystals with acetonitrile as the guest. Intertwined 1D and 2D solvent channel networks were observed in the PyHOC4-hexane and in the PyHO3C3-H2O-MeOH crystal structures, respectively, while the other pseudopolymorphs of PyHOC4 and PyHO3C3 and all PyC4 crystal forms exhibit closely packed crystal structures without open channels.
Immunity to Backscattering of Bulk Waves in Topological Acoustic Superlattices
Authors: Deymier P. A. , Vasseur Jérome O. , Runge K. , Khanikaev A. , Alù A.
Published: April 2024

We herein investigate the scattering of orthogonal counterpropagating waves and one-way propagating bulk waves in discrete acoustic superlattices subjected to a scattering potential applied to one of the superlattice unit cells. We demonstrate theoretically that the orthogonality of counterpropagating modes does not provide robust protection against backscattering. By contrast, the one-way propagating modes do satisfy a no-reflection condition, i.e., they exhibit immunity to backscattering, for a wide range of applied scattering potentials, which represent defects and disorder.
Mycorrhizal Fungi of Phalaenopsis japonica (Orchidaceae) and Their Role in Seed Germination and Seedling Development
Authors: Chamara R. M. S. Ruwan , Rammitsu Kento , Minobe Mutsumi , Kinoshita Akihiko , Kotaka Nobuhiko , Yukawa Tomohisa , Ogura-Tsujita Yuki
Published: April 2024

Epiphytic orchids comprise 68% of vascular epiphytes globally; nevertheless, many are endangered. One such epiphytic orchid is Phalaenopsis japonica, which is widely used in the floricultural industry. This study aimed to identify the mycorrhizal fungi of adult P. japonica and their roles in seed germination and seedling development. Root samples were collected from 32 adults across 4 sites in southern Japan, and mycorrhizal fungi were identified using Sanger and high-throughput sequencing (HTS). The results show phylogenetically diverse mycobionts, mainly Ceratobasidiaceae (CE) and Tulasnellaceae (TU), with dominant OTUs designated CE6 and CE22. Sanger sequencing found 9 OTUs, 4 CE, and 5 TU; HTS detected 22 OTUs, 4 CE, 16 TU, and 2 Serendipitaceae. Seeds inoculated with CE6 improved germination and protocorm development compared with other strains. In addition, asymbiotic seedlings inoculated with CE6, CE22, and TU18 displayed varying effects in growth, with CE6 being the most notable. While TU18 did not promote seed germination, it effectively promoted leaf development in seedlings. Overall, Ceratobasidiaceae was predominantly associated with seed germination, seedling growth, and the adult stages, with CE6 and CE22 becoming the primary partners throughout the life history of P. japonica. Our findings illuminate mycorrhizal symbiosis in epiphytic habitats, offering conservation and commercial production insights.
From Generation to Reuse: A Circular Economy Strategy Applied to Wind Turbine Production
Authors: Caramelo Ana Rita , Santos Paulo , Lima Tânia Miranda
Published: April 2024

The environmental impact of wind turbine rotor blades, both during manufacturing and at the end of their life cycle, can be significant. The aim of this study was to define and test a methodology for recycling the waste resulting from their production. Particles of waste from the mechanical machining of rotor blades, which were made up of a glass fibre/epoxy matrix mixture, were used to produce toe caps for use by the footwear industry. The addition of 1 wt.% of particles improved the mechanical properties of the epoxy matrix, with a 5.50% improvement in tension and an 8% improvement in stiffness. Characterisation of the laminates, manufactured by hand lay-up technique, revealed that in the three-point bending tests, the additive laminates showed improvements of 18.60% in tension, 7.50% in stiffness, and 10% in deformation compared to the control laminate. The compression test showed that the additive glass fibre toe cap had greater resistance to compression than the control glass fibre toe cap, with a reduction in deformation of 23.10%. The toe caps are suitable for use in protective footwear according to European standard EN ISO 20346:2022. They guaranteed protection against low-velocity impacts at an energy level of at least 100 J and against compression when tested at a compression load of at least 10 kN.