bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of Gelatin and Crosslinker Concentrations in a Gelatin/Alginate-Based Bioink with Potential Applications in a Simplified Skin Model
Authors: Cavallo Aida , Radaelli Giorgia , Al Kayal Tamer , Mero Angelica , Mezzetta Andrea , Guazzelli Lorenzo , Soldani Giorgio , Losi Paola
Published: February 2025

Three-dimensional bioprinting allows for the fabrication of structures mimicking tissue architecture. This study aimed to develop a gelatin-based bioink for a bioprinted simplified skin model. The bioink printability and chemical-physical properties were evaluated by varying the concentrations of gelatin (10, 15, and 20%) in a semi-crosslinked alginate-based bioink and calcium chloride (100, 150, and 200 mM) in post-printing crosslinking. For increasing the gelatin concentration, the gelatin-based formulations have a shear thinning behavior with increasing viscosity, and the filament bending angle increases, the spreading ratio value approaches 1, and the shape fidelity and the printing resolution improve. However, the formulation containing 20% of gelatin was not homogeneous, resulting also in poor printability properties. The morphology of the pores, degradation, and swelling depend on gelatin and CaCl2 concentrations, but not in a significant way. The samples containing 15% of gelatin and crosslinked with 150 mM CaCl2 have been selected for the bioprinting of a bilayer skin model containing human fibroblasts and keratinocytes. The model showed a homogeneous distribution of viable and proliferating cells over up to 14 days of in vitro culture. The gelatin-based bioink allowed for the 3D bioprinting of a simplified skin model, with potential applications in the bioactivity of pro-reparative molecules and drug evaluation.
Elucidating the Mechanism of Coumarin Homodimerization Using 3-Acetylcoumarin Derivatives
Authors: Simeonova Kristina B. , Koleva Ana I. , Petkova-Yankova Nevena I. , Zlatanova Anna-Mariya R. , Lozanova Vesela , Nikolova Rositca D. , Petkov Petko St.
Published: February 2025

The current study is a continuation of our previous investigations into the radical homodimeric reaction mechanism of 3-acetylcoumarin. In the current study, the effects of different substituents on the coumarin ring of 3-acetylcoumarin are investigated both experimentally and theoretically. Several 3-acetylcoumarin derivatives (substituted at C-6, C-7, and C-8) were tested in the optimized reaction conditions under ultrasound irradiation, and biscoumarin species were isolated and characterized. The elucidation of the substituent’s effect was further investigated by means of DFT calculations (free-energy calculations, NBO analysis), both in the initial substituted coumarins and in the formed radicals. It was observed that the presence of substituents at the C-6 and C-8 positions in the coumarin moiety would not affect significantly the formation of a radical, while a group at position C-7 could either stabilize or destabilize the formed radical depending on the electronic properties of the substituent.
Recent Developments in Stereoselective Reactions of Sulfoxonium Ylides
Authors: O’Shaughnessy Ciarán , Mondal Mukulesh , Kerrigan Nessan J.
Published: February 2025

This review probes the recent developments in stereoselective reactions within the area of sulfoxonium ylide chemistry since the early 2000s. An abundance of research has been applied to sulfoxonium ylide chemistry since its emergence in the early 1960s. There has been a continued effort since then with work in traditional areas, such as epoxidation, aziridination and cyclopropanation. Efforts have also been applied in novel areas, such as olefination and insertion reactions, to develop stereoselective methodologies using organocatalysis and transition metal catalysis. The growing research area of interrupted Johnson–Corey–Chaykovsky reactions is also described, whereby unexpected stereoselective cyclopropanation and epoxidation methodologies have been developed. In general, the most observed mechanistic pathway of sulfoxonium ylides is the formal cycloaddition: (2 + 1) (e.g., epoxides, cyclopropanes, aziridines), (3 + 1) (e.g., oxetanes, azetidines), (4 + 1) (e.g., indanones, indolines). This pathway involves the formation of a zwitterionic intermediate through nucleophilic addition of the carbanion to an electrophilic site. An intramolecular cyclization occurs, constructing the cyclic product. Insertion reactions of sulfoxonium ylides to X–H bonds (e.g., X = S, N or P) are also observed, whereby protonation of the carbanion is followed by a nucleophilic addition of X, to form the inserted product.
Copper(I)-Photocatalyzed Addition of Trichloromethanesulfenyl Chloride to Olefinic Compounds
Authors: Petek Nejc , Zorko Tilen , Škrinjar Martin , Grošelj Uroš , Svete Jurij , Kočar Drago , Štefane Bogdan
Published: February 2025

Atom transfer radical addition (ATRA) reactions are essential transformations in organic synthetic chemistry that enable the atom-economic difunctionalization of abundant olefin feedstocks. In this way, a rich chemical space can be opened up by well-planned combinations of simple starting materials. To build an efficient photocatalytic transformation, the reactivity of trichloromethanesulfenyl chloride toward alkenes and alkynes was investigated under photocatalytic Cu(I) reaction conditions. In this study, we found that trichloromethanesulfenyl chloride can be added to a series of olefins (such as styrenes and electron-rich and -poor olefins) in the presence of 1 mol% [Cu(dmp)2]BF4 photocatalyst and blue LED irradiation, producing α-chloro trichloromethylthioethers in good yields. Experimental and theoretical (DFT) mechanistic studies are consistent with the proposed radical chain mechanism of transformation. This study may serve as a valuable reference for the development of new coupling reactions that are economical and highly efficient processes.
Tocotrienols in Eleven Species of Hypericum Genus Leaves
Authors: Lazdiņa Danija , Mišina Inga , Górnaś Paweł
Published: February 2025

Saint John’s worts or goatweeds are mostly perennial flowering plants in the Hypericaceae family, formerly under the Clusiaceae family. Teas and macerations of the plants are common in traditional medicines and modern depression and cancer therapies. The most notable bioactive compounds in Hypericum are hyperforin and hypericin. While Hypericum contains a variety of carotenoid and phenolic compounds, which are well documented, there is little available information on tocopherols and almost none on tocotrienols. Considering the frequency of tocotrienol derivatives in Clusiaceae species, this study investigates and reports the presence of tocotrienols in eleven Hypericum species’ leaves: H. hircinum, H. hookerianum, H. calycinum, H. xylosteifolium, H. densifolium, H. prolificum, H. kalmianum, H. frondosum, H. olympicum, and two hybrids: H. × moserianum and H × ‘Rowallane’. Eight tocopherol and tocotrienol forms (α, β, γ, δ) were detected in the leaves, predominantly containing α-tocopherol. Tocotrienol content was most significant in Myriandra section species and was highest in H. prolificum (22.90 ± 0.63 mg 100 g−1), while the highest tocotrienol proportion was observed in H. × ‘Rowallane’ (54.12% of total tocochromanols) and H. prolificum (37.27% of total tocochromanols). The results demonstrated significant tocochromanol accumulation in Hypericum leaves.
Study on the Extraction of Rare Earth Elements (REEs) from Phosphogypsum Using Gluconobacter oxydans Culture Solution
Authors: Zhang Jiangang , Qi Zhuo , He Zijian , Zhang Xinyue , Zhang Qinglian , Su Xiangdong
Published: February 2025

With the rapid development of modern industry, particularly in the fields of electric vehicles and renewable energy technologies, the demand for rare earth elements (REEs) has surged dramatically. Phosphogypsum (PG), which is an industrial waste product generated during the production of phosphoric acid through the sulfuric acid process, is rich in REEs. However, traditional chemical leaching methods pose environmental pollution and resource wastage issues. This study aims to explore the feasibility and optimal conditions for bioleaching REEs from phosphogypsum using Gluconobacter oxydans (G. oxydans). The phase composition and components of phosphogypsum, as well as the growth characteristics and leaching efficiency of G. oxydans, were analyzed in detail using SEM, EDS, XRD, and XRF techniques. Experimental results indicate that G. oxydans can effectively leach REEs from phosphogypsum under conditions of 28 °C, an agitation speed of 150 rpm, and a liquid-to-solid ratio of 4:1, with a maximum leaching efficiency of 24.67%. Moreover, it is revealed in the study that G. oxydans exhibits selectivity in leaching REEs. Specifically, the leaching efficiency for Nd is significantly enhanced at low pH values. This research provides a theoretical basis and practical application example for the efficient and environmentally friendly recovery of REEs from phosphogypsum.
Modulation of the S/HgCl2 Ratio for the Synthesis and Conversion of Cinnabar and Metacinnabar
Authors: Hao Qilong , Zhang Zhehan , Zhang Wenyuan , Yu Zongren , Shi Yanping , Zhang Haixia , Su Bomin
Published: February 2025

Cinnabar has been used as a red pigment for centuries, but its degradation significantly impacts the aesthetic quality of historical paintings, particularly murals. Therefore, investigating the preparation method and transformation process of HgS is highly significant for mural research. In this study, we compared different sulfur sources for HgS synthesis and precisely synthesized α-HgS and β-HgS by adjusting the S/HgCl2 ratio. SEM and XRD analyses under optimal conditions demonstrated that spherical β-HgS-1.2 exhibited significant morphological differences in comparison with α-HgS-1.0 and α-HgS-1.5. Elemental analysis of HgS was conducted using XPS and ICP-MS for qualitative and quantitative insights. Based on the potential mechanism of cinnabar discoloration, two strategies for converting black β-HgS to α-HgS were proposed and successfully implemented by adding sulfur or HgCl2.
Impact of Silver and Copper Oxide Nanoparticles on Anaerobic Digestion of Sludge and Bacterial Community Structure
Authors: Abdulsada Zainab K. , Kibbee Richard , Princz Juliska , Örmeci Banu
Published: February 2025

The effect of metal nanoparticles on the anaerobic digestion of sludge and the sludge bacterial community are still not well-understood, and both improvements and inhibitions have been reported. This study investigated the impact of 2, 10, and 30 mg/g TS silver and copper oxide nanoparticles (AgNPs and CuONPs) on the mesophilic anaerobic digestion of sludge and the bacterial community structure. The reactors were monitored for changes in tCOD, sCOD, TS, VS, biogas generation, and cell viability. Also, the relative abundance and taxonomic distribution of the bacterial communities were analyzed at the phylum and genus levels, including the genera involved in anaerobic digestion. Both AgNPs and CuONPs exhibited some inhibition on anaerobic digestion of sludge and biogas generation, and the inhibition was more evident at higher concentrations. CuONPs had a stronger inhibitory effect compared to AgNPs. After the introduction of AgNPs and CuONPs, cell viability initially decreased over the first two weeks but recovered after that. At high concentrations, AgNPs and CuONPs decreased the overall bacterial diversity, and inhibited the dominant bacterial species, allowing those in less abundance to flourish. The relative abundance of the bacteria responsible for hydrolysis and acidogenesis increased and the relative abundance of acetogenic bacteria decreased with higher AgNP and CuONP concentrations. The majority of the parameters measured for monitoring the anaerobic digestion performance and bacterial community were not statistically significant at 2 mg/g TS of AgNPs and CuONPs, which represents naturally present concentrations in wastewater sludge that are below the USEPA ceiling concentration limits.
Dietary Micronutrient Intake During Pregnancy Is Suboptimal in a Group of Healthy Scottish Women, Irrespective of Maternal Body Mass Index
Authors: Jarvie Eleanor M. , Lovegrove Julie A. , Weech Michelle , Freeman Dilys J. , Meyer Barbara J.
Published: February 2025

Background/Objectives: A balanced nutritious diet is vital during pregnancy for both the mother and the baby. The aims of this longitudinal study were to (1) determine any differences in macro- and micronutrient intakes in a group of UK women during pregnancy (and in the post-partum period) who were overweight or obese (BMI mean (SD) 31.1 (2.9)) at antenatal booking appointment compared with women who were within the ideal BMI range (BMI mean (SD) 22.1 (1.9)) and (2) determine the proportion of women who met the Harmonized Average Requirements (H-AR) during pregnancy. Methods: Forty-two participants attended four clinic visits: three during pregnancy, one in each trimester (V1, V2, and V3), and one 12 weeks post-partum (V4). Dietary intake was assessed by 24 h diet recall and analysed using DietPlan6. Results: There were no differences in energy and macronutrient intakes between overweight/obese and lean women. During pregnancy, the overweight/obese women consumed a mean (SD) of 3238 (941) sodium (mg per day), which was approximately 10% higher compared to 2934 (732) sodium (mg per day) in the lean group (p = 0.015). Dietary and supplemental intakes of the sodium to potassium ratio was 21% higher in overweight/obese women compared to the lean women, p = 0.0031 (mean (SD) of 1.17 (0.35) versus 0.93 (0.28), respectively). Virtually all women did not meet the H-AR for niacin, folate, and vitamin D through dietary intake alone. Conclusions: The ‘eat better and not more’ message during pregnancy is supported.
Improving Irritable Bowel Syndrome (IBS) Symptoms and Quality of Life with Quebracho and Chestnut Tannin-Based Supplementation: A Single-Centre, Randomised, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Molino Silvia , De Lellis Lorenza Francesca , Morone Maria Vittoria , Cordara Marcello , Larsen Danaè S. , Piccinocchi Roberto , Piccinocchi Gaetano , Baldi Alessandra , Di Minno Alessandro , El-Seedi Hesham R. , Sacchi Roberto , Daglia Maria
Published: February 2025

Background/Objectives: Irritable Bowel Syndrome (IBS) is a disorder of the gut-brain axis for which the gastroenterologist is most often consulted. Gastrointestinal symptoms and decreased quality of life lead to a considerable burden of disease. The exact causes of IBS are not well understood, and no standard therapy has been established. The primary outcome of the study focused on the improvements of the IBS symptoms assessed through the validated questionnaire IBS-Severity Scoring System (IBS-SSS). Similarly, secondary outcomes geared towards the improvement of the quality of life (IBS-Quality of Life (IBS-QoL) and the Gastrointestinal Quality of Life Index (GIQLI)) and specific IBS symptoms (bloating, abdominal distension, feeling of heaviness, abdominal pain, and flatulence), were assessed through self-administered questionnaires. Intestinal habits (consistency and frequency of depositions) through subject stratification into diarrhoea (IBS-D), constipation (IBS-C), mixed type (IBS-M), as well as the treatment tolerability were also evaluated. Methods: A randomised, placebo-controlled, double-blind, clinical trial was conducted on 156 enrolled IBS patients (79 female and 77 male), aged 18–70 years, randomised (1:1 allocation ratio) to receive either two capsules per day of the food supplement (containing 480 mg of a complex of tannin extracts) or a placebo for 56 days. Linear random intercept mixed models (LMM) were used to analyse all experimental variables Results: Supplementation resulted in a significant improvement (p < 0.05) in the primary outcome IBSS score, with respect to the placebo group, changing the IBS condition going from mild (242.3 ± 89.8) to moderate (148.1 ± 60.6). Similarly, all indicators concerning quality of life, and specific intestinal symptoms resulted in a significant improvement (p < 0.05). Furthermore, the tannin-based treatment showed the ability to modulate the response to different symptomatology such as diarrhoea and constipation, without side effects being reported. Conclusions: The use of a supplement based on chestnut and quebracho tannins presents great application potential in the management of IBS-related disorders, with the peculiarity of resolving opposite symptoms, such as diarrhoea and constipation, indiscriminately.
Sunflower Oil Fortified with Vitamins D and A and Sunflower Lecithin Ameliorated Scopolamine-Induced Cognitive Dysfunction in Mice and Exploration of the Underlying Protective Pathways
Authors: Tang Xue , Zhu Chengkai , Liu Tristan C. , Zhu Rongxiang , Deng Guoliang , Zhou Peng , Liu Dasong
Published: February 2025

The incidence of cognitive disorders is increasing globally, with a reported prevalence of over 50 million individuals affected, and current interventions offer limited efficacy. This study investigates the effects of sunflower oil fortified with sunflower lecithin, vitamin D, and vitamin A on scopolamine-induced cognitive dysfunction in mice and explores the underlying mechanisms. The incidence of cognitive disorders, such as Alzheimer’s disease, is increasing yearly, and current interventions offer limited efficacy. Therefore, this research aims to evaluate the cognitive improvement effects of the three added functional factors on mice with learning and memory impairments, along with the associated molecular mechanisms. Behavioral tests, biochemical assays, and real-time quantitative polymerase chain reaction (RT-qPCR) were utilized to examine the intervention effects of these functional factors on scopolamine-induced cognitive impairment in mice. The results revealed that the groups treated with sunflower lecithin and vitamin D significantly enhanced the mice’s exploratory behavior, working memory, and spatial memory, with increases of 1.6 times and 4.5 times, respectively, in the open field and novel object recognition tests (VD group). Additionally, these treatments reduced levels of inflammatory markers and IL-6, increased antioxidant GSH levels, and decreased oxidative stress marker MDA levels, with all effects showing significant differences (p < 0.01). The effects were further enhanced when vitamin A was combined with these treatments. Transcriptomic analysis demonstrated that the intervention groups had markedly improved learning and memory abilities through upregulation of key gene expression levels in the PI3K-AKT signaling pathway, cholinergic pathway, and folate biosynthesis pathway. These findings provide a theoretical basis for the development of nutritionally fortified edible oils with added sunflower lecithin, vitamin D, and vitamin A, which may help prevent and ameliorate cognitive disorders.
Cross-Cultural Comparison of Burnout, Insomnia and Turnover Intention Among Nurses in Eastern and Western Cultures During the COVID-19 Pandemic: Protective and Risk Factors
Authors: Frey Eveline , Chong Yuen-Yu , Chien Wai-Tong , Gloster Andrew T.
Published: February 2025

Background/Objectives: The COVID-19 pandemic has adversely impacted the mental health of nurses worldwide. Nurse burnout results from chronic workplace stress and is characterized by exhaustion, negative or cynical feelings about work, and a diminished sense of accomplishment. This can lead to turnover intention. Therefore, psychological capacities, such as psychological flexibility, that could help nurses regulate and minimize the impact should be studied. This study aimed to compare burnout, insomnia and turnover intention among nurses from an Eastern and Western cultural context and to investigate the role of psychological flexibility as a protective factor against mental health and related problems such as insomnia and turnover intention. Methods: Nurses from Hong Kong (n = 158) and Switzerland (n = 294) involved in patient care during the COVID-19 pandemic participated in an online mental health survey. Results: We observed high levels of burnout, subthreshold insomnia and turnover intention in nurses from both Switzerland and Hong Kong, with higher burnout rates among Hong Kong nurses and higher turnover intentions among nurses from Switzerland, and that psychological flexibility is a crucial factor that may protect nurses from burnout and insomnia. Conclusions: The nurses from both regions involved in patient care one year after the onset of the pandemic suffered from poor mental health. Psychological flexibility was identified as a critical factor in improving nurses’ mental health. The results of this study should be incorporated into health promotions for nursing professionals and help develop specific and effective interventions for practical nursing application.
Epidemiology and Genetic Evolutionary Analysis of Influenza Virus Among Children in Hainan Island, China, 2021–2023
Authors: Chang Meng , Shi Shengjie , Jin Yan , Wang Gaoyu , Peng Ruoyan , An Jing , Huang Yi , Hu Xiaoyuan , Tang Chuanning , Niu Yi , Tian Xiuying , Deng Wanxin , Tang Cheng , Cui Xiuji , Chan Jasper Fuk-Woo , Jia Yibo , Yin Feifei
Published: February 2025

Background: During the COVID-19 pandemic, we continuously monitored the epidemiology of influenza virus among pediatric patients from January 2021 to December 2023 in Hainan Island, China. Methods: In this study, we collected 54,974 nasopharyngeal swab samples for influenza A Virus (IAV) testing and 53,151 samples for influenza B Virus (IBV) testing from pediatric outpatients. Additionally, we also collected 19,687 nasopharyngeal swab samples from pediatric inpatients for IAV and IBV testing. Outpatient samples were screened for influenza viruses (IVs) infection by the colloidal gold method. Targeted Next-Generation Sequencing (tNGS) was used to detect influenza virus infections in inpatients. Influenza virus types were identified by analyzing the HA/NA partial regions. Results: The findings revealed a significant decrease in the infection rate of IBV over the specified period, while the infection rate of IAV exhibited a rising trend. Additionally, B/Victoria lineage was the dominant epidemic strain in 2021, while the epidemic strains in 2022 and 2023 underwent a dynamic transformation from A/H3N2 to A/H1N1. Phylogenetic analysis revealed close relationships among the circulating strains. Nonetheless, because the sample size is limited, additional research is required. Conclusions: Our findings suggest that the predominant types of influenza viruses in the pediatric population are undergoing dynamic changes, influenced by the implementation and relaxation of non-pharmaceutical intervention measures. These findings highlight the need for adaptive influenza vaccination and containment strategies, particularly in tropical regions like Hainan, where climate and public health policies significantly impact viral transmission patterns. The insights gained from this study could inform more effective public health strategies in similar regions to mitigate the impact of influenza outbreaks in the future.
Immunomodulatory Effects of Atractylodes lancea in Healthy Volunteers with Dosage Prediction for Cholangiocarcinoma Therapy: A Modelling Approach
Authors: Saeheng Teerachat , Karbwang Juntra , Na-bangchang Kesara
Published: February 2025

Background and Aims: According to a recent study on the immunomodulatory activity of Atractylodes lancea (Thunb.) DC. (AL) in healthy Thai subjects, AL significantly inhibited the production of key pro-inflammatory cytokines while stimulating the production of immune cells. However, no maximum tolerated dose (MTD) and phase 2A dosage regimens were reported. The study aimed to evaluate the immunomodulatory effects of Atractylodes lancea (Thunb.) DC. (AL) in healthy subjects, and to recommend optimal dose regimens for intrahepatic cholangiocarcinoma (iCCA) based on toxicity criteria. Methods: A physiologically based pharmacokinetic (PBPK) model, combined with the toxicological approach and the immunomodulatory effect, was used for dose-finding. The safety and efficacy of each AL regimen were evaluated based on the previous study. At least a once-daily dose of 1000 mg AL significantly suppressed the production of all pro-inflammatory cytokines while significantly increasing the number of peripheral immune cells. Results: The developed PBPK model predicted the clinically observed data well. No significant differences in SII index values were found, but a difference in the lymphocyte-monocyte ratio was found on day 4. The dosage regimen for phase 2A is a once-daily dose of 1500 or 2000 mg. Preliminary results in phase 2A revealed that a once-daily dose of 2000 mg had a significantly higher median overall survival, progression-free survival, disease control rate, and inhibition of increased tumor size without toxicities compared with control. Conclusions: A PBPK model, in conjunction with a toxicological approach, could assist in finding the potential dosage regimens for a clinical study, including herbal medicine.
Breakthrough Advances in Beta-Lactamase Inhibitors: New Synthesized Compounds and Mechanisms of Action Against Drug-Resistant Bacteria
Authors: Huang Ya-Si , Zhou Hong
Published: February 2025

Beta-lactam drugs hold a central place in the antibacterial arsenal, and the production of beta-lactamases by drug-resistant bacteria has severely compromised the effectiveness of nearly all available beta-lactams. Therefore, in the face of the increasing threat of drug resistance, the combined use of beta-lactamase inhibitors (BLIs) with beta-lactam antibiotics is crucial for treating infections caused by drug-resistant bacteria. Hence, the development of BLIs has always been a hot topic in the field of medicinal chemistry. In recent years, significant progress has been made in screening active drugs by enhancing the affinity of inhibitors for enzymes and the stability of their complexes, based on the design concept of competitive inhibitors. Here, we review the effects and mechanisms of newly synthesized beta-lactamase inhibitors on various BLIs in recent years, to provide ideas for the development of subsequent beta-lactamase inhibitors.
Microencapsulation of Probiotics for Enhanced Stability and Health Benefits in Dairy Functional Foods: A Focus on Pasta Filata Cheese
Authors: D’Amico Vita , Cavaliere Mariasimona , Ivone Marianna , Lacassia Chiara , Celano Giuseppe , Vacca Mirco , la Forgia Flavia Maria , Fontana Sergio , De Angelis Maria , Denora Nunzio , Lopedota Angela Assunta
Published: February 2025

Probiotics provide significant health benefits, but their viability is often compromised during production, storage, and passage through the gastrointestinal tract. These challenges hinder their effective incorporation into functional applications, particularly in dairy functional foods, in which factors such as acidity, oxygen exposure, and storage conditions negatively impact cell survival. The focus was on functional dairy foods, particularly on pasta filata cheeses. Indeed, the use of probiotics in pasta filata cheeses presents significant challenges due to the specific manufacturing processes, which encompass the application of high temperatures and other harsh conditions. These factors can adversely affect the viability and availability of probiotic microorganisms. However, microencapsulation has emerged as a promising solution, offering a protective barrier that enhances probiotic stability, improves survival rates, and facilitates targeted release in the gastrointestinal environment. This review examines the pivotal role of microencapsulation in stabilising probiotics for functional applications, emphasising its relevance in high-value food systems. Functional applications, including foods designed to offer essential nutritional benefits and promote host health, play a crucial role in disease prevention and immune system support, reducing the risk of infections and other physiological impairments. Key microencapsulation technologies are analysed, focusing on their benefits, limitations, and challenges related to scalability and industrial implementation. Additionally, this review discusses strategies to optimise formulations, ensure the sensory quality of final products, and explore future opportunities for expanding innovative applications that align with growing consumer demand for health-promoting solutions.
Quality-of-Life Assessment and Pharmacokinetic Study in Hemophilia A Patients Undergoing Prophylactic Treatment
Authors: Kotsiou Nikolaos , Evangelidis Paschalis , Bolios Michail , Tragiannidis Konstantinos , Kalmoukos Panagiotis , Ntova Zacharo , Chissan Sofia , Vakalopoulou Sofia , Gavriilaki Eleni
Published: February 2025

This study evaluates the health-related quality of life (HRQoL) among patients with hemophilia A currently undergoing prophylactic treatment at the Hemophilia Center of Northern Greece. Using the Haem-A-QoL questionnaire, we assessed various HRQoL dimensions in a cohort of 29 adult male patients, analyzing the impact of age, disease severity, and treatment regimens. The results revealed that younger patients (18–30 years old) exhibited significantly better overall HRQoL scores (total score of 25.36) compared to older age groups (37.81 for the 31–45 group and 43.71 in the 45+ group), particularly in the physical health (29.16 vs. 48.43 vs. 58.57) and mental well-being domains (25 vs. 37.11 vs. 41.07). Interestingly, moderate hemophilia patients reported lower HRQoL (42.31) than those with severe form (34.85), suggesting unique challenges in managing their condition. The ’Sports/Free Time’ domain had the highest scores (65.81), indicating significant limitations in physical activities in the everyday lives of affected individuals. However, better outcomes were observed in the mental dimension (36.09), work/study (34.88), family planning (10.68), and relationships aspects (16.67), where our cohort reported very low scores compared to similar studies, indicating a significantly better quality of life in these domains. These findings highlight the importance of personalized psychosocial support and targeted interventions to address the specific needs of hemophilia patients, particularly in enhancing physical activity opportunities and managing the psychological burden of moderate hemophilia. The study contributes valuable insights into the HRQoL of hemophilia patients and underscores the necessity for tailored approaches to improve patient outcomes across all dimensions of life.
Continuous-Wave Room-Temperature External Cavity Quantum Cascade Lasers Operating at λ~8.5 μm
Authors: Wang Zixian , Lin Yuzhe , Ma Yuan , Wan Chenyang , Dong Fengxin , Zhou Xuyan , Zhang Jinchuan , Liu Fengqi , Zheng Wanhua
Published: February 2025

External cavity quantum cascade lasers (EC-QCLs) utilizing the Littrow configuration and operating at an approximate wavelength of 8.5 μm have been successfully demonstrated in continuous wave operations at room temperature. Our work provides ideas and experimental support for the optimization of the EC-QCL which indicate optimal EC-QCL performance with an external cavity length of 25 cm and investigates the impact of various parameters, including injection current and temperature on the performance of the EC-QCL. In the absence of anti-reflection (AR) coating, the tuning range at 25 °C extends up to 103.3 cm−1, while the maximum side mode suppression ratio (SMSR) reaches 30.8 dB, accompanied by a full width half maximum linewidth (FWHM) of 0.76 nm.
Phylogenetic and Expression Analysis of SBP-Box Gene Family to Enhance Environmental Resilience and Productivity in Camellia sinensis cv. Tie-guanyin
Authors: Gao Yusen , Wen Yingxin , Lin Qinmin , Feng Yizhuo , Shi Xinying , Xiao Siyao , Tumukunde Elisabeth , Zheng Kehui , Cao Shijiang
Published: February 2025

Tieguanyin tea, a renowned oolong tea, is one of the ten most famous teas in China. The Squamosa Promoter Binding Protein (SBP)-box transcription factor family, widely present in plants, plays a crucial role in plant development, growth, and stress responses. In this study, we identify and analyze 22 CsSBP genes at the genome-wide level. These genes were distributed unevenly across 11 chromosomes. Using Arabidopsis thaliana and Solanum lycopersicum L. as model organisms, we constructed a phylogenetic tree to classify these genes into six distinct subfamilies. Collinearity analysis revealed 20 homologous gene pairs between AtSBP and CsSBP, 21 pairs between SiSBP and CsSBP, and 14 pairs between OsSBP and CsSBP. Cis-acting element analysis indicated that light-responsive elements were the most abundant among the CsSBP genes. Protein motif, domain, and gene architecture analyses demonstrated that members of the same subgroup shared similar exon–intron structures and motif arrangements. Furthermore, we evaluated the expression profiles of nine CsSBP genes under light, shade, and cold stress using qRT-PCR analysis. Notably, CsSBP1, CsSBP17, and CsSBP19 were significantly upregulated under all three stresses. This study provides fundamental insights into the CsSBP gene family and offers a novel perspective on the mechanisms of SBP transcription factor-mediated stress responses, as well as Tieguanyin tea’s adaptation to environmental variations.
Weighted Gene Correlation Network Analysis Reveals Key Regulatory Genes Influencing Selenium Enrichment and Yield with Exogenous Selenite in Tartary Buckwheat
Authors: Ye Xueling , Mei Linsen , Gan Zhen , Wang Zhiqiang , Sun Wenjun , Fan Yu , Liu Changying , Wu Qi , Wan Yan , Wu Xiaoyong , Xiang Dabing
Published: February 2025

Selenium (Se) is an essential trace element for human health, and dietary Se intake is an effective supplement. Rich in nutrients and functional components with potential for Se enrichment, Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) is a Se-biofortified cereal. To determine the optimal Se treatment concentration and fully understand its effects on Tartary buckwheat, sodium selenite (Na2SeO3) in different concentrations was sprayed onto leaves of Tartary buckwheat at the initial flowering stage. Agronomic and yield-related traits and Se enrichment were analyzed between CK and treatments. The results showed that Na2SeO3 concentrations of 3.0 and 6.0 mg/L significantly increased the contents of Se and starch in the grains, the 1000-grain weight, the number of grains per plant, and the yield. The 6.0 mg/L treatment had the best effect. Transcriptome and weighted gene co-expression network analyses showed that selenite promoted chlorophyll synthesis and photoelectron transport by upregulating chlorophyll synthase (CHLG) and protein CURVATURE THYLAKOID 1B (CURT1B) levels, improving photosynthesis, increasing sucrose synthesis and transport in leaves and starch synthesis and accumulation in grains, and promoting grain-filling and yield. These changes were regulated by genes related to photosynthesis, sucrose, and starch metabolism-related genes, including CAB3C, HPR3, SUS5, BAM9, SS3, SWEET1, and SWEET12. Selenite absorption in Tartary buckwheat was regulated by aquaporin genes NIP1-1 and PIP1-5. Selenite transport was regulated by the inorganic phosphate transporter gene PHT1-1, and organic Se transport was controlled by the proton-dependent oligopeptide transporters NPF3.1 and NPF4.6. Methionine gamma-lyase (MGL) was involved in selenocompound metabolism. This study identified the best spraying scheme for enhancing Se content in the grains. It also revealed the regulatory genes responding to selenite absorption, transport, and metabolism and the regulatory pathways promoting yield in Tartary buckwheat. These results provide technical guidance and theoretical support for producing high-yielding and Se-enriched Tartary buckwheat.
Melatonin-Induced Transcriptome Variation of Sweet Potato Under Heat Stress
Authors: Wang Mengzhao , Zhou Yang , Liang Bei , Kumar Sunjeet , Zhao Wenjie , Liu Tianjia , Li Yongping , Zhu Guopeng
Published: February 2025

Melatonin (MT) has been widely recognized for its ability to mitigate the effects of abiotic stress and regulate plant development. In this study, we investigated the role of exogenous MT in enhancing heat tolerance in sweet potato, with a particular focus on its capacity to alleviate heat stress-induced damage. MT treatment significantly reduced oxidative stress, as evidenced by decreased levels of hydrogen peroxide, superoxide ions, and malondialdehyde (MDA), all of which were elevated under heat stress. To uncover the underlying mechanisms, RNA sequencing was performed on three experimental groups: control (CK), heat stress alone (HS), and MT pre-treatment followed by heat stress (MH). A total of 3491, 3280, and 1171 differentially expressed genes (DEGs) were identified in the CK vs. HS, CK vs. MH, and HS vs. MH comparisons, respectively. MT treatment notably modulated the expression of genes involved in redox regulation and nicotinate and nicotinamide metabolism. Moreover, MT enhanced the expression of genes associated with key signaling pathways, including mitogen-activated protein kinases (MPK3) and plant hormone signal transduction components, such as ethylene response factor (ERF). These findings offer novel insights into the mechanisms by which exogenous MT enhances heat tolerance in sweet potato, highlighting its role in regulating antioxidant systems, metabolic pathways, and hormone signaling. This study presents valuable strategies for improving crop resilience to heat stress.
Plant Stem Cells in Cosmetic Industry
Authors: Gardiki Vassiliki , Pavlou Panagoula , Siamidi Angeliki , Papageorgiou Spyridon , Papadopoulos Apostolos , Iakovou Kriton , Varvaresou Athanasia
Published: February 2025

It is interesting to note that some of the most lucrative commercial products available today are derived from plant cell cultures. Apple, grape, ginger, rice, and other plant stem cells have been successfully and extensively utilized in cosmetic preparations all over the world. The advantages of plant cell suspensions over field-grown complete plants, which exhibit developmental stages of growth, plant age, and organ-specific differences, include sustainability, lack of pesticide residues, and independence from climate fluctuations. The procedure of extracting and purifying physiologically active compounds from plant cell cultures is significantly streamlined because of the possibility that these chemicals may be released into the intercellular gaps or wasted media through the cell walls and membrane. Upon downstream processing from the cells, the released chemicals exhibit minimal losses and a high degree of purity. Overall, the practical interest is in creating high-quality, sustainable, and innovative skincare solutions that meet both consumer needs and environmental concerns while driving the cosmetic industry toward more advanced biotechnological approaches. Our review intends to show the advantages of plant stem cells in cosmetic preparations.
Global Dynamics of Grassland FVC and LST and Spatial Distribution of Their Correlation (2001–2022)
Authors: Miao Zhenggong , Chen Ji , Wang Chuanglu , Zhang Shouhong , Ma Yinjun , Dong Tianchun , Zhao Yaojun , Shi Rui , Zhao Jingyi
Published: February 2025

Fractional Vegetation Cover (FVC) and Land Surface Temperature (LST) are critical indicators for assessing grassland ecosystems. Based on global remote sensing data for FVC and LST from 2001 to 2022, this study employs the Mann–Kendall trend test and Spearman correlation analysis to explore the dynamic changes in and spatial distribution patterns of both variables. The results indicate that the FVC is increasing in regions such as Europe, the eastern southern Sahara, western India, eastern South America, western and southern North America, and central China. However, it is decreasing in southern Canada, the central United States, and northern Australia. Significant increases in LST are observed in subarctic regions and the Tibetan Plateau, attributed to polar warming effects associated with global climate change. Conversely, the LST is decreasing in central China, eastern coastal Australia, and southern Africa. The global FVC–LST relationship exhibits the following four distinct spatial distribution patterns: (1) FVC increase and LST increase (Type 1), (2) FVC increase and LST decrease (Type 2), (3) FVC decrease and LST increase (Type 3), and (4) FVC decrease and LST decrease (Type 4). Type 1, covering 33.72%, is primarily found in high-latitude and high-altitude areas, such as subarctic regions and the Tibetan Plateau. Type 2, the largest group (46.98%), is mainly located in eastern North America, eastern South America, and southern Africa. Type 3, which comprises 18.72%, is concentrated in arid and semi-arid regions, while Type 4, representing only 0.59%, lacks clear spatial distribution patterns.
Differential Primary Seed and Fruit Dispersal Mechanisms and Dispersal Biomechanics in Invasive Dehiscent and Indehiscent-Fruited Lepidium Species
Authors: Mohammed Said , Steinbrecher Tina , Leubner-Metzger Gerhard , Mummenhoff Klaus
Published: February 2025

This study explores primary dispersal, which involves diaspores’ detachment directly from mature plants, and secondary dispersal, which encompasses any further dispersal occurring after the primary dispersal. A comparison of the primary dispersal vectors of the invasive dehiscent fruit producing Lepidium campestre and the indehiscent fruit producing Lepidium draba was conducted. These vectors were examined in relation to the native and introduced distribution ranges of the species, and regarding biomechanical forces required detaching the fruits from mature plants. Our findings reveal that rainfall and animal contact serve as primary dispersal vectors for Lepidium campestre, while animal contact is rarely involved in primary dispersal of Lepidium draba. Primary dispersal is more important for Lepidium campestre than Lepidium draba, which requires significantly greater force for fruit detachment. While previous studies indicate wind and rainfall as major secondary dispersal vectors for Lepidium draba, our results suggest secondary dispersal via mucilage on seeds is more crucial for this species. The strong reliance of Lepidium draba on secondary dispersal, enabling long-distance dispersal, could contribute to its invasion success, linking it to the species’ geographic distribution. Understanding these different dispersal strategies is essential for effective management of invasive species.
N-Alkanes in Permafrost Peatlands
Authors: Pastukhov Alexander , Kaverin Dmitry , Loiko Sergey
Published: February 2025

In this study, high-performance liquid chromatography (HPLC) methods were utilised to identify and quantify C21–C33 n-alkanes in permafrost peatlands located within the Eastern European and Western Siberian cryolithozone. The total content of n-alkanes in Europe is 7.4 times higher compared to Siberian permafrost peatlands, and was estimated at 282 ± 145 (range from 74 to 709) μg/kg and 38 ± 12 (10–66) μg/kg, respectively. In the European cryolithozone, CPI alkane 9.5 ± 2.4 (3.7–18.6) and HPA 0.10 ± 0.03 (0.05–0.23) indicate a relatively higher share of higher plants and a higher stage of peat decomposition decree, with 6.9 ± 2.1 (3.1–12.9) and 0.15 ± 0.05 (0.06–0.29) in the Siberian region. In contrast, the Western Siberian peat plateaus were formed under conditions of constant excess moisture, a distinction from the Eastern European peatlands, where moisture and permafrost conditions were subject to constant change. This is further corroborated by the values of Paq, C23/C29 and C23(C27 + C31), which are 0.90 ± 0.05 (0.69–0.99); 11.1 ± 8.9 (0.84–61.6); 1.53 ± 0.80 (0.21–4.72) and 0.47 ± 0.12 (0.08–0.71); 0.64 ± 0.32 (0.08–1.48); and 0.43 ± 0.21 (0.04–1.26), respectively. The n-alkanes and peat physicochemical properties show no significant correlation. In the European part, permafrost degradation occurred repeatedly during the warming periods. Nevertheless, only slight subsidence of the permafrost table was observed, and peat continued to accumulate (up to 0.1 mm/year) in the West Siberian peat plateaus. Consequently, the variation in the quantitative and qualitative composition of n-alkanes in permafrost peatlands is determined not only by the different botanical composition of the plant remains forming the peat strata, but also by the consequence of lower mean annual temperatures in Western Siberia compared to the European nNortheast, and such a climatic difference persisted throughout the Holocene.
Hyperspectral Imaging and Machine Learning for Huanglongbing Detection on Leaf-Symptoms
Authors: Dong Ruihao , Shiraiwa Aya , Ichinose Katsuya , Pawasut Achara , Sreechun Kesaraporn , Mensin Sumalee , Hayashi Takefumi
Published: February 2025

Huanglongbing is one of the most destructive diseases of citrus worldwide. Infected trees die due to the absence of practical cures. Thus, the removal of HLB-infected trees is one of the principal HLB managements for the regulation of disease spread. Here, we propose a non-destructive HLB detection method based on hyperspectral leaf reflectance. In total, 72 hyperspectral leaf images were collected in an HLB-invaded citrus orchard in Thailand and each image was visually distinguished into either any HLB symptom appearance (symptomatic) or no symptoms (asymptomatic) on the leaf. Principal component analysis was applied on the hyperspectral data and revealed 16 key wavelengths at red-edge to near-infrared regions (715, 718, 721, 724, 727, 730, 733, 736, 930, 933, 936, 939, 942, 945, 957, and 997 nm) that were characteristically differentiated in the symptomatic group. Seven models learnt on the spectral data at these 16 wavelengths were examined for the potential to separate these two image groups: random forest, decision tree, support vector machine, k-nearest neighbor, gradient boosting, logistic regression, linear discriminant. F1-score was employed to select the best-fit model to distinguish the two categories: random forest achieved the best score of 99.8%, followed by decision tree and k-nearest neighbor. The reliability of the visual grouping was evaluated by nearest neighbor matching and permutation test. These three models separated the two image categories as precisely as PCR results, indicating their potential as alternative tool instead of PCR.
Influence of Plasticizers on Cross-Linking Process, Morphology, and Properties of Lignosulfonate-Filled Rubber Compounds
Authors: Kruželák Ján , Džuganová Michaela , Kvasničáková Andrea , Preťo Jozef , Hronkovič Ján , Hudec Ivan
Published: February 2025

Calcium lignosulfonate as a biopolymer component was incorporated into acrylonitrile butadiene rubber in the amount—50 phr. Four low-molecular organic molecules—1,4-butanediol, ethylene glycol, and two glycerols with different purity—were used as plasticizers. They were applied in rubber compounds in the amount ranging from 5 to 30 phr. The influence of the plasticizers on the curing process, cross-link density, morphology, and physical–mechanical properties was investigated. The blooming of plasticizers was also under observation. The results revealed that the application of plasticizers resulted in the deceleration of the curing kinetics and reduction in torque increments, pointing to the decrease in the rubber compounds’ viscosity. This was subsequently confirmed by the rheological measurements. The plasticizers softened the rubber matrix as well as the biopolymer filler. The higher the polarity of the plasticizer, the higher the plasticizing effect on lignosulfonate. The plasticizing effect increased in the following order: 1,4-butanediol < ethylene glycol < glycerols. Softened lignosulfonate formed small, soft filler-like domains well distributed within the rubber matrix. Good compatibility and adhesion between the rubber and the biopolymer on their interface was observed, leading to the enhancement in the tensile characteristics of the vulcanizates plasticized with ethylene glycol and glycerols.
Bioactive Polymer Materials with Antibacterial Properties: An Editorial
Authors: Huq Md. Amdadul , Akter Shahina
Published: February 2025

Bioactive materials have a wide range of applications, and bioactive materials with antibacterial properties, in particular, have attracted significant medical interest [...]