bluuebunny/crossref_metadata_2025 huggingface dataset:
Dolly Parton’s Mythologised Persona, Collective Life Writing, and Building a Home for LGBTQ+ Listeners in Country Music
Authors: Barker James
Published: December 2022

This article conceptualises persona and life writing as being collectively constituted to explore multiplicity in the way country artist Dolly Parton’s persona can be constructed by LGBTQ+ audiences. I use the idea of mythmaking, where myths are neither true nor false, to explore how Parton’s persona and life writing challenge dominant narratives and assumptions around LGBTQ+ belonging in country music, a genre that is increasingly being re-evaluated in terms of its LGBTQ+ representation and queer resonances. Parton’s persona is deeply invested in her constructed life story that represents a collective mythology around the Smoky Mountains of East Tennessee, Appalachia, and country music. This paper uses close readings of Parton’s songs ‘Coat of Many Colors’ and ‘My Tennessee Mountain Home’ to explore how these texts enable LGBTQ+ listeners to anchor themselves within the life-writing practices of her country music and Appalachia. ‘Coat of Many Colors’ uses an episode from Parton’s life story to work through class and regional representations that also have the potential to resonate with LGBTQ+ experiences through the renegotiation of narratives of shame that become reworked into pride and acceptance. My reading of ‘My Tennessee Mountain Home’ expands on this using the idea of home and the affective role this has within queer narratives to explore how Parton situates LGBTQ+ experiences within the genre of country music. The final part of this article considers the importance of anti-racism and intersectional critiques of overly romanticised narratives around Parton to ensure multiplicity when conceptualising the role of persona in Parton’s work.
‘Talent Offends, Genius Terrifies’
Authors: Rontsch Marc
Published: December 2022

Christopher Langford James (1952 – 2008) was a Zimbabwean-born composer, orchestrator and pianist, whose style conflated traditional European musical textures with southern African instrumentation, rhythms, and harmonies. His compositions include works such as Four Portraits for Pianoforte in Four Movements (1982), Songs of Lamentation and Remonstration (1985), Images of Africa (1987), and Paradise Regained (1999).  While my research on James’s life and music has uncovered multiple angles for critical inquiry, the dominant narrative that emerged from interviews with his family, and discussions with colleagues, was that of mental illness.
This article argues that mental illness – while being a very real condition experienced by James – can simultaneously be understood as doing persona work. Through the intersecting frameworks of persona studies and life writing, this article critically interrogates the understanding of stereotypical concepts of mental illness within the construct of what Kim Barbour terms ‘artistness’. Through critical examination of how mental illness is understood and interpreted as a central construction of artistness, this article speaks to the complexity of the construction of James’s biography, and how the narrative performance of James’s mental illness can be understood within the framework of persona studies.
Evaluation of interventions to prevent vasovagal reactions among whole blood donors: rationale and design of a large cluster randomised trial.
Authors: McMahon Amy , Kaptoge Stephen , Walker Matthew , Mehenny Susan , Gilchrist Philippe T , Sambrook Jennifer , Akhtar Naim , Sweeting Michael , Wood Angela M , Stirrups Kathleen , Chung Ryan , Fahle Sarah , Johnson Elisha , Cullen Donna , Godfrey Rosemary , Duthie Shannon , Allen Louise , Harvey Paul , Berkson Michael , Allen Elizabeth , Watkins Nicholas A , Bradley John R , Kingston Nathalie , Miflin Gail , Armitage Jane , Roberts David J , Danesh John , Angelantonio Emanuele Di
Published: December 2022

Background: Vasovagal reactions (VVRs) are the most common acute complications of blood donation. Responsible for substantial morbidity, they also reduce the likelihood of repeated donations, and are disruptive and costly for blood services. Although blood establishments worldwide have adopted different strategies to prevent VVRs (including water loading and applied muscle tension [AMT]), robust evidence is limited. The Strategies to Improve Donor Experiences (STRIDES) trial aims to reliably assess the impact of four different interventions to prevent VVRs among blood donors.
Methods: STRIDES is a cluster-randomised cross-over/stepped-wedge factorial trial of four interventions to reduce VVRs involving about 1.4 million whole blood donors enrolled from all 73 blood donation sites (mobile teams and donor centres) of National Health Service Blood and Transplant (NHSBT) in England. Each site (“cluster”) has been randomly allocated to receive one or more interventions during a 36-month period, using principles of cross-over, stepped-wedge and factorial trial design to assign the sequence of interventions. Each of the four interventions are compared to NHSBT’s current practices: (i) 500ml isotonic drink before donation vs current 500ml plain water; (ii) 3-minutes rest on donation chair after donation vs current 2 minutes; (iii) new modified AMT vs current practice of AMT; and (iv) psychosocial intervention using preparatory materials vs current practice of nothing. The primary outcome is the number of in-session VVRs with loss of consciousness (i.e., episodes involving loss of consciousness of any duration, with or without additional complications). Secondary outcomes include all in-session VVRs (i.e., with and without loss of consciousness) and delayed VVRs (i.e., those occurring after leaving the venue).
Discussion: The STRIDES trial should yield novel information about interventions, singly and in combination, for the prevention of VVRs, with the aim of generating policy-shaping evidence to help inform blood services to improve donor health, donor experience, and service efficiency.
Trial registration: ISRCTN: 10412338
The malignancy suppression and ferroptosis facilitation of BCL6 in gastric cancer mediated by FZD7 repression are strengthened by RNF180/RhoC pathway
Authors: Guo Shiwei , Wang Pengliang , Kou Fan , Wu Zizhen , Zhang Nannan , Zhao Zhenzhen , Nie Yongzhan , Deng Jingyu , Yang Lili
Published: December 2022

Background
B-cell lymphoma 6 (BCL6) is a transcription repressor that plays a tumor suppressor or promoting role in various tumors. However, its function and molecular mechanism in gastric cancer (GC) remain unclear. Ferroptosis, a novel programmed cell death, is closely related to tumor development. In this research, we aim to explore the role and mechanism of BCL6 in malignant progression and ferroptosis of gastric cancer.
Methods
Firstly, BCL6 was identified as an important biomarker that attenuated the proliferation and metastasis of GC through tumor microarrays and confirmed in GC cell lines. RNA sequence was performed to explore the downstream genes of BCL6. The underlying mechanisms were further investigated by ChIP, dual luciferase reporter assays and rescue experiments. Cell death, lipid peroxidation and MDA level were detected to determine the effect of BCL6 on ferroptosis and the mechanism was revealed. CHX, MG132 treatment and rescue experiments were used to explore the upstream regulatory mechanism of BCL6.
Results
Here we showed that BCL6 expression was significantly decreased in GC tissues, and patients with low BCL6 expression showed more malignant clinical features and poor prognosis. The upregulation of BCL6 may significantly inhibit the proliferation and metastasis of GC cells in vitro and in vivo. In addition, we found that BCL6 directly binds and transcriptionally represses Wnt receptor Frizzled 7 (FZD7) to inhibit the proliferation, metastasis of GC cells. We also found that BCL6 promoted lipid peroxidation and MDA level to facilitate ferroptosis of GC cells by FZD7/β-catenin/TP63/GPX4 pathway. Furthermore, BCL6 expression and function was regulated by the ring finger protein 180 (RNF180)/ras homolog gene family member C (RhoC) pathway, which had been elucidated to be involved in significantly mediating the proliferation and metastasis of GC cells.
Conclusions
In summary, BCL6 should be considered a potential intermediate tumor suppressor to inhibit the malignant progression and induce ferroptosis, which may be a promising molecular biomarker for further mechanistic investigation of GC.
EffEctiveness of Prophylactic fOam dressings in the prevention of saCral pressure injuries in at-risk hospitalised patients: The EEPOC Trial
Authors: Walker Rachel M , Chaboyer Wendy , Cooke Marie , Whitty Jennifer A , Thalib Lukman , Lockwood Ishtar , Latimer Sharon , Campbell Jill , Probert Rosalind , Gillespie Brigid M
Published: December 2022

Background
Prophylactic dressings are increasingly used to prevent pressure injuries in hospitalised patients. However, evidence regarding the effectiveness of these dressings is still emerging. This trial aims to determine the clinical and cost-effectiveness of a prophylactic silicone foam border dressing in preventing sacral pressure injuries in medical-surgical patients.
Methods
This is a multicentre, pragmatic, parallel group, randomised controlled trial. A sample size of 1,320 was calculated to have > 90% power to detect a 5% difference in the primary outcome at an alpha of 0.05. Adult patients admitted to participating medical-surgical wards are screened for eligibility: ≥18 years; admitted to hospital within the previous 36 hours; expected length of stay of ≥ 24 hours and assessed high risk for hospital acquired pressure injury. Consenting participants are randomly allocated to either prophylactic silicone foam dressing intervention or usual care without any dressing as the control group via a web-based randomisation service independent of the trial. Patients are enrolled across three Australian hospitals. The primary outcome is the cumulative incidence of patients who develop a sacral pressure injury. Secondary outcomes include time to sacral pressure injury, incidence of severity (stage) of sacral pressure injury, cost-effectiveness of dressings, and process evaluation. Participant outcomes are assessed daily for up to 14 days by blinded independent outcome assessors using deidentified, digitally modified sacral photographs. Those who develop a sacral pressure injury are followed for an additional 14 days to estimate the costs of pressure injury treatment. Analysis of clinical outcomes will be based on intention-to-treat, per-protocol, and sensitivity analyses.
Discussion
This trial aims to provide definitive evidence on the effect prophylactic dressings have on development of hospital acquired sacral pressure injuries in medical-surgical patients. A parallel economic evaluation of pressure injury prevention and treatment will enable evidence-informed decisions and policy. The inclusion of a process evaluation will help to explain the contextual factors that may have a bearing on the results of the trial including acceptability of the dressings to patients and staff. The trial commenced 5 March 2020 but has been significantly delayed due to COVID-19.
Trial registration:
ANZCTR, prospectively registered 22 May 2019: ACTRN12619000763145
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042
Authors: Alcántara Cristina , Pérez Marta , Huedo Pol , Altadill Tatiana , Espadaler-Mazo Jordi , Arqués Juan Luís , Zúñiga Manuel , Monedero Vicente
Published: December 2022

Polyphosphate (poly-P) biosynthesis in bacteria has been linked to many physiological processes and has been characterized as an interesting functional molecule involved in intestinal homeostasis. We determined the capacity for poly-P production of 18 probiotic strains mainly belonging to Bifidobacterium and former Lactobacillus genera, showing that poly-P synthesis is a strain-specific trait dependent on the availability of phosphate and the growth phase. Bifidobacteria were especially capable of poly-P synthesis and poly-P kinase (ppk) genes were identified in their genomes together with a repertoire of genes involved in phosphate transport and metabolism. In Bifidobacterium longum KABP042, the strain we found with highest poly-P production, variations in ppk expression were linked to growth conditions and presence of phosphate in the medium. Moreover, the strain produced poly-P in presence of breast milk and human milk oligosaccharide lacto-N-tetraose increased the amount of poly-P synthesized. Compared to KABP042 supernatants low in poly-P, exposure of Caco-2 cells to KABP042 supernatants rich in poly-P resulted in decreased epithelial permeability and increased barrier resistance, induction of epithelial protecting factors such as HSP27 and enhanced expression of tight junction protein genes. These results highlight the role of bifidobacteria-derived poly-P as a strain-dependent functional factor acting on epithelial integrity.
Recent irreversible retreat of Pine Island Glacier
Authors: Reed Brad , Green J. A. Mattias , Jenkins Adrian , Gudmundsson G. Hilmar
Published: December 2022

Pine Island Glacier (PIG), a part of the West Antarctic marine ice sheet, has recently undergone substantial changes including acceleration, grounding-line retreat and thinning. Theoretical arguments suggest that marine ice sheets can become unstable, entering phases of irreversible retreat. Modelling work indicates this as a possible future scenario for PIG. However, to date, no firm evidence is available of an unstable retreat phase having occurred. Here we present a modelling study, validated by observational data, of a rapid PIG retreat in the 1970s, and demonstrate that it was self-enhancing and irreversible. Results show PIG had crossed a tipping point by the early 1970s whereby further grounding-line retreat was inevitable despite temporal reversal in climatic conditions. The irreversible phase had ended by the early 1990s after almost 40 km of retreat and 0.34 mm added to global sea level, causing PIG to become the main contributor from the Antarctic Ice Sheet.
Whole-exome sequencing analysis identifies novel variants associated with Kawasaki disease susceptibility
Authors: Zhang Xing , Sun Ying , Meng Lijuan , Ye Caixia , Han Huifeng , Zhang Tiesong , Feng Yue , Li Jianxiao , Duan Lifen , Chen Yanfei
Published: December 2022

Background: Kawasaki disease (KD) is an acute pediatric vasculitis affecting genetically susceptible infants and children. Although the pathogenesis of KD remains unclear, growing evidence links genetic susceptibility to the disease. To explore the genes associated with susceptibility in KD, we conducted whole-exome sequencing of KD children from Yunnan province, China.
Methods: We retrospectively evaluated the data from 93 KD patients and 91 non-KD controls who underwent whole-exome sequencing.
Results: In this study, we successfully collected and identified relationships between two significant rare variant genes (MYH14 and RBP3) and Kawasaki disease through the genotype/allele frequency analysis (odds ratio [OR], 8.3945 to 13.1963; p-value, 0.0025 to 0.0346). The eight of 20 KD patients all have heterogeneous variants in Chr19: 50281727 (G/A), Chr19: 50223086 (G/A), Chr19: 50280044 (T/G), Chr19: 50301707 (C/A), Chr19: 50301790 (C/T), Chr19: 50293670 (C/T), Chr19: 50292282 (C/T), Chr19: 50244260 (C/T), and the remaining twelve cases had heterogeneous variants in Chr10: 47351134 (G/A), associated with retinitis pigmentosa, which may be associated to one clinal manifestation of KD.
Conclusion: This study suggested that two genes MYH14 and RBP3 may be associated with KD susceptibility in the population from Yunnan province.
Effects of Lead and Lean in Multi-Axis Directed Energy Deposition
Authors: Elsayed Omar , Adapa Venkata Surya Karthik , Kersten Samuel , Vaughan Derek , Masuo Christopher , Kim Myong Joon , Feldhausen Thomas , Saldana Christopher , Kurfess Thomas
Published: December 2022

The present study examines the effect of varying laser incidence angles on textural, microstructural, and geometric characteristics of directed energy deposition (DED) processed materials, providing a more comprehensive outlook on participating laser-matter interaction phenomena and ultimately devise strategies to ameliorate print performance. In this study, single-layer, single-/multi-track specimens were processed to examine the effect of non-orthogonal angular configurations on bead morphology, microstructure, phase composition and textural representation of DED-processed 316L stainless steel materials. It was observed that bead size decreased at increasing lead and lean angles. Asymmetry in the distribution of the bead morphology as a function of lead angle indicates better catchment for acute lead angle configurations over obtuse configurations. No significant differences in phase composition, texture, and microstructure were observed in moderate off-axis configurations. When penetration depth for the deposits was below 20 µm, columnar structures dominated the microstructure of the deposited material. At deeper penetration depths, columnar and equiaxed structures were observed at the bead-substrate interface and center of the bead, respectively. Compared to powder-blown DED, wire-DED dilution profiles were found to be asymmetric in both orthogonal and non-orthogonal wire DED samples.
Combining host and rumen metagenome profiling for selection in sheep: prediction of methane, feed efficiency, production, and health traits
Authors: Hess Melanie , Zetouni Larissa , Hess Andrew S. , Budel Juliana , Dodds Ken G. , Henry Hannah M. , Brauning Rudiger , McCulloch Alan F. , Hickey Sharon M. , Johnson Patricia L. , Elmes Sara , Wing Janine , Bryson Brooke , Knowler Kevin , Hyndman Dianne , Baird Hayley , McRae Kathryn M. , Jonker Arjan , Janssen Peter H. , McEwan John C. , Rowe Suzanne J.
Published: December 2022

Background
Rumen microbes break down complex dietary carbohydrates into energy sources for the host and are increasingly shown to be a key aspect of animal performance. Host genotypes can be combined with microbial DNA sequencing to predict performance traits or traits related to environmental impact, such as enteric methane emissions. Metagenome profiles were generated from 3,139 rumen samples, collected from 1,200 dual purpose ewes, using Restriction Enzyme-Reduced Representation Sequencing (RE-RRS). Phenotypes were available for methane (CH4) and carbon dioxide (CO2) emissions, the ratio of CH4 to CH4 plus CO2 (CH4Ratio), feed efficiency (Residual Feed Intake: RFI), liveweight at the time of methane collection (LW), liveweight at 8 months (LW8), fleece weight at 12 months (FW12) and parasite resistance measured by faecal egg count (FEC1). We estimated the proportion of phenotypic variance explained by host genetics and the rumen microbiome, as well as prediction accuracies for each of these traits.
Results
Incorporating metagenome profiles increased the variance explained and prediction accuracy compared to fitting only genomics for all traits except for CO2 emissions when animals were on a grass. Combining the metagenome profile with host genotype from lambs explained more than 70% of the variation in methane emissions and residual feed intake. Predictions were generally more accurate when incorporating metagenome profiles compared to genetics alone, even when considering profiles collected at different ages (lamb vs adult), or on different feeds (grass vs lucerne pellet). A reference-free approach to metagenome profiling performed better than metagenome profiles that were restricted to capturing genera from a reference database. We hypothesise that our reference-free approach is likely to outperform other reference-based approaches such as 16S rRNA gene sequencing for use in prediction of individual animal performance.
Conclusions
This paper shows the potential of using RE-RRS as a low-cost, high-throughput approach for generating metagenome profiles on thousands of animals for improved prediction of economically and environmentally important traits. A reference-free approach using a microbial relationship matrix from log10 proportions of each tag normalized within Cohort (i.e., the group of animals sampled at the same time) is recommended for future predictions using RE-RRS metagenome profiles.
Impact of age on indication for chemotherapy in early breast cancer patients in Germany
Authors: Hoffmann Ann Sophie , Hennigs André , Feisst Manuel , Moderow Mareike , Heublein Sabine , Deutsch Thomas Maximilian , Togawa Riku , Schäfgen Benedikt , Wallwiener Markus , Golatta Michael , Heil Jörg , Riedel Fabian
Published: December 2022

Purpose: Today, the decision to treat patients with chemotherapy for early breast cancer (EBC) is made based on the patient’s individual risk stratification and tumor biology. In cases with chemotherapy indication, the neoadjuvant application (NACT) is the preferred option in comparison with primary surgery and adjuvant chemotherapy (ACT). Age remains a relevant factor in the decision-making process. The aim of the present study was to illustrate the impact of age on the use of systemic therapy in clinical routine.
Methods: The study separately analyzed chemotherapy use among six age cohorts of EBC patients who had been treated at 104 German breast units between January 2008 and December 2017.
Results: In total, 124,084 patients were included, 46,279 (37.3%) of whom had received chemotherapy. For 44,765 of these cases, detailed information on treatment was available. Within this cohort, chemotherapy was administered as NACT to 14,783 patients (33.0%) and as ACT to 29,982 (67.0%) patients. Due to the higher prevalence of unfavorable tumor subtypes, younger patients had a higher rate of chemotherapy (≤29y: 74.2%; 30–39y: 71.3%) and a higher proportion of NACT administration (<29y: 66.9%; 30–39y: 56.0%) in comparison with elderly patients, who had lower rates for overall chemotherapy (60–69y: 37.5%; ≥70y: 17.6%) and NACT (60–69y: 25.5%; ≥70y: 22.8%). Pathologic complete response was higher in younger than in older patients (≤ 29y: 30.4% vs. ≥70y: 16.7%), especially for HER2− subtypes.
Conclusion: The data from the nationwide German cohort reveal relevant age-dependent discrepancies concerning the use of chemotherapy for EBC.
n = 250 words
ANRIL promotes the regulation of colorectal cancer on lymphatic endothelial cells via VEGF-C and is the key target for Pien Tze Huang to inhibit cancer metastasis
Authors: Lin Jiumao , Huang Bin , Lu Yao , Ni Zhuona , Liu Jinhong , He Yanbin , An Honglin , Ye Feimin , shen Jiayu , Lin Minghe , Chen Yong
Published: December 2022

lncRNA ANRIL is an oncogene, however the role of ANRIL in the regulation of colorectal cancer on human lymphatic endothelial cells (HLECs) is remain elusive. Pien Tze Huang (PZH, PTH) a Tradition Chinese Medicine (TCM) as an adjunctive medication could inhibit the cancer metastasis, however the mechanism still uncovering. We used network pharmacology, subcutaneous and orthotopic transplanted colorectal tumors models to determine the effect of PZH on tumor metastasis. Differential expressions of ANRIL in colorectal cancer cells, and stimulating the regulation of cancer cells on HLECs by culturing HLECs with cancer cells’ supernatants. Network pharmacology, transcriptomics, and rescue experiments were carried out to verify key targets of PZH. We found PZH interfered with 32.2% of disease genes and 76.7% of pathways, and inhibited the growth of colorectal tumors, liver metastasis, and the expression of ANRIL. The overexpression of ANRIL promoted the regulation of cancer cells on HLECs, leading to lymphangiogenesis, via upregulated VEGF-C secretion, and alleviated the effect of PZH on inhibiting the regulation of cancer cells on HLECs. Transcriptomic, network pharmacology and rescue experiments show that PI3K/AKT pathway is the most important pathway for PZH to affect tumor metastasis via ANRIL. In conclusion, PZH inhibits the regulation of colorectal cancer on HLECs to alleviate tumor lymphangiogenesis and metastasis by downregulating ANRIL dependent PI3K/AKT/VEGF-C pathway.
iMPI – portable human-sized Magnetic Particle Imaging Scanner for real-time endovascular Interventions
Authors: Vogel Patrick , Rückert Martin , Greiner Christoph , Günther Johanna , Reichl Teresa , Kampf Thomas , Bley Thorsten , Behr Volker , Herz Stefan
Published: December 2022

Minimally invasive endovascular interventions have become an important tool for the treatment of cardiovascular diseases such as ischemic heart disease, peripheral artery disease, and stroke. X-ray fluoroscopy and digital subtraction angiography are used to precisely guide these procedures, but they are associated with radiation exposure for patients and clinical staff. Magnetic Particle Imaging (MPI) is an emerging imaging technology using time-varying magnetic fields combined with magnetic nanoparticle tracers for fast and highly sensitive imaging. In recent years, basic experiments have shown that MPI has great potential for cardiovascular applications. However, current MPI scanners were too large, slow and expensive, and had a very small field of view (FOV), which limited further translational research. Here, we present a portable interventional MPI (iMPI) scanner dedicated for real-time endovascular interventions free of ionizing radiation. It uses a novel field generator approach with a very large FOV and an application-oriented open design enabling hybrid approaches with conventional X-ray-based angiography. The feasibility of a real-time iMPI-guided percutaneous transluminal angioplasty (PTA) is shown in a realistic dynamic human-sized leg model.
CLOOME: contrastive learning unlocks bioimaging databases for queries with chemical structures
Authors: Klambauer Günter , Fernandez Ana Sanchez , Rumetshofer Elisabeth , Hochreiter Sepp
Published: December 2022

Currently, bioimaging databases cannot be queried by chemical structures that induce the phenotypic effects captured by the image. We present a novel retrieval system based on contrastive learning that is able to identify the chemical structure inducing the phenotype out of $\sim$2,000 candidates with a top-1 accuracy $>$70 times higher than a random baseline.
Cost-effectiveness of serial C-reactive protein (CRP) measurements in hospitalized patients with bacteremia
Authors: Hanuka Aviv , Schwartz Yuval , Bdolah-Abram Tali , Assous Marc , Yinnon Amos , Munter Gavriel
Published: December 2022

Background: CRP is increasingly used as inflammatory marker in sepsis.
Aim of study: Assessment of the cost-effectiveness of CRP in the treatment of patients with bacteremia.
Methods: This was a retrospective study of two patient cohorts, one in 2015 (cohort A) and the other in 2018 (cohort B), each included 200 consecutively diagnosed patients with proven bacteremia. We assessed the following outcome markers: antibiotic use, duration of admission and in-hospital mortality.
Results: Baseline demographic and clinical data of the cohorts were similar. In cohort A, a single CRP test was obtained from 5% of the patients, while in cohort B, 95% of patients had CRP tests with a mean of 5.7 tests/person. Empiric, appropriate antibiotic treatment, increased from 72% in cohort A to 75% in cohort B (NS). Duration of antibiotic treatment did not change (10±8 days). The in-hospital mortality rate decreased from 38.5% in cohort A to 30.5% in cohort B (NS), as did mortality <7 days after diagnosis of bacteremia, from 17.5% to 14% respectively)(NS).
Conclusion: We found statistically insignificant differences between the cohorts. It is difficult to establish a causal relationship between the described changes and the widespread use of serial CRP tests. There is currently no evidence that supports serial use of CRP tests in the treatment of patients with bacteremia.
Internet Gaming Disorder and Mental Health in Pakistani youth: A Path Analysis with Impulsivity and Emotional Intelligence
Authors: Khalid Amna , Mukhtar Nadia
Published: December 2022

Background
Over the past decade the research on Internet gaming disorder IGD in youth has increased substantially due to its impact on young people’s mental health. One of the established risk factor of IGD is impulsivity. However, there is very little literature exploring its pathway of association with emotional intelligence leading to IGD especially in context of Pakistan.
Methods
We collected an online sample of 387 Pakistani, 15–24 years-olds who were involved in online gaming. Participants filled Barratt Impulsiveness Scale; BIS; WHO-5 wellbeing scale; Wong and Law Emotional Intelligence Scale (WLEIS) and Internet Gaming Disorder Scale–Short-Form (IGDS9-SF).
Results
The levels of IGD were high (58.9%) among youth involved in online gaming in Pakistan. We used Mplus to run path analysis showing that impulsivity partially mediates the association between emotional intelligence and IGD which then reduced wellbeing. Furthermore, impulsivity showed very strong association with IGD.
Conclusion
Interventions enhancing emotional intelligence among youth may account for some of the negative repercussions of impulsivity. There is need for further research in this area among youth from low and middle income countries to support the study findings. Association between IGD and participants background characteristics have also be discussed.
Combined use of voltage mapping and right ventriculography to guide ablation for arrhythmogenic right ventricular cardiomyopathy ventricular tachycardia：a case report
Authors: Yang Chengying , Liao Lei , Zeng Di , Liu Xiongtao , peng Chen , Wei Yan , Fan Xinrong
Published: December 2022

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a familial inherited cardiomyopathy associated with ventricular arrhythmias. With the development of molecular biology, histology, imaging, and other diagnostic techniques, the diagnosis rate and incidence of ARVC have gradually increased. However, ARVC remains rare in clinical practice. Currently, there is no specific treatment to eliminate arrhythmia. In this case report, we describe a clinical case of radiofrequency ablation in the treatment of ARVC with ventricular tachycardia and discuss the relevant literature.
Development and validation of a mobile application for graphical representation of pediatric urological anomalies: The Uro Map software
Authors: Ocáriz Rodrigo Campos , Miranda Márcio Lopes , Dias Ulisses Martins , Takei Danilo Yoiti , Pegolo Patrícia Traballi de Carvalho , Bustorff-Silva Joaquim Murray
Published: December 2022

Purpose:
The Uro Map application for smartphones was developed to help healthcare providers in the treatment of children with urological malformations, raising the possibility of graphically representing each urinary tract unit as an schematic illustration and associating it with relevant data from the patient’s medical history, producing an illustration representing the child’s urinary tract status that can be both archived and/or shared among caregivers. This app was submitted to a group of caregivers to test its accuracy and the results of this survey was the basis of the present report.
Methods:
Common pediatric urological situations were simulated through the application, generating ten images, which were attached to a form. For each figure, a multiple-choice question was assigned, with only one correct answer per question. The forms were sent to 875 Pediatric Surgeons and 400 Pediatric Nephrologists through their national scientific associations.
Results:
174 Pediatric Surgeons, 53 Pediatric Nephrologists, 18 Pediatric Surgery Residents, 26 urologists and 12 other healthcare providers answered the questionnaire. The latter 2 groups were formed due to the sharing of the form by the participants themselves. The mean score in each group was: pediatric surgeons 9.51, pediatric nephrologists 9.52, pediatric surgery residents 9.88, urologists 9.69 and other participants 8.8.
Conclusion:
The Uro Map application was successfully developed and based on the participants’ scores, it can be considered effective to represent the most diverse pediatric urological disorders.
End to End Delay Analysis of Dynamic Cooperative Spectrum Sensing Mechanism in IEEE 802.22 WRAN
Authors: Bino J , Bhuvaneswari P T V
Published: December 2022

IEEE 802.22 Wireless Regional Area Networks (WRAN) utilizes the Cognitive Radio dynamic spectrum access technique in TV bands. When allocating available spectrum to Secondary Users (SUs), in the absence of Primary Users (PUs), it is essential to ensure the Quality of Service (QoS) of SUs. The motivation behind this research is to devise a mechanism to ensure the QoS of SUs. The End to End delay (EED) experienced by SU through the devised mechanism need to be kept within the threshold suggested by WRAN standard. To achieve the above requirement, Dynamic Cooperative Spectrum Sensing (DCSS) is developed in this research. Two entities namely Spectrum Manager (SM) in Base Station (BS) and Spectrum Automation (SA) in SUs are engaged to realize them. They does the sensing of channel occupancy by the PUs. Further, a concept of Urgent Coexistence Situation (UCS) notification is issued by BS to indicate the re-arrival of PUs to SUs. Buffer mechanism is provisioned to restore the ongoing traffic of SUs until further resource allocation is accomplished. This strategy assures guaranteed data delivery of SUs traffic. However, this may introduce EED. Hence, this research investigates the performance of the developed DCSS mechanism in Netsim version 12 Simulator. The scenario with 1 Base Station, 3 PUs and 1 pair of SUs is constructed. Three different SUs traffic namely CBR, Voice and Video is considered. The impact of modulation schemes, occurrence of PUs traffic on average EED is analysed. From the simulation results, the modulation scheme that provides average EED within the threshold as per IEEE 802.22 is identified, thereby ensuring the QoS of the SUs.
Combustion regimes in turbulent non-premixed flames for space propulsion
Authors: Sanchis Daniel Martinez , Sternin Andrej , Haidn Oskar , Tajmar Martin
Published: December 2022

Direct numerical simulations of non-premixed fuel-rich methane-oxygen flames at 20 bar are conducted to investigate the turbulent mixing burning of gaseous propellants in rocket engines. The reacting flow is simulated using the EBI-DNS solver, within the OpenFOAM frame. The transport of species is resolved with finite rate chemistry, using a complex skeletal mechanism that entails 21 species. Two different flames at low and high Reynolds numbers are considered to study the sensitivity of the flame dynamics to turbulence. Regime markers are used to measure the probability of the flow to burning in premixed and non-premixed conditions at different regions. The local heat release statistics are studied to understand the drivers in the development of the turbulent diffusion flame. Despite the eminent non-premixed configuration, a significant amount of combustion takes place in premixed conditions. Premixed combustion is viable in both lean and fuel-rich regions, relatively far from the stoichiometric line. It is found that a growing turbulent kinetic energy is detrimental to combustion in fuel-rich premixed conditions. This is motivated by the disruption of the local premixed flame front, which promotes fuel transport into the diffusion flame. In addition, at downstream positions, higher turbulence enables the advection of methane into the lean core of the flame, enhancing the burning rates in these regions. Hence, the primary effect of turbulence is to increase the fraction of propellants burnt in oxygen-rich and near stoichiometric conditions. As a consequence, the mixture fraction of the products shifts towards lean conditions, influencing combustion completion at downstream positions.
How Low Can You Go? Broiler Breast Muscle Possesses a Mitochondrial Content of Just 2%
Authors: Soumeh Elham A. , Bottje Walter G. , Hudson Nicholas J.
Published: December 2022

In 1977 the pectoralis major (breast) muscle mitochondrial content of domestic broiler chickens, Gallus gallus domesticus, was estimated at 4.1%. This very low value is unsurprising in light of the bird’s unique muscle fibre composition, being exclusively composed of low aerobic capacity type IIB fibres. However, in the subsequent ~ 45 years broilers have been further modified by sustained intensive genetic selection on feed conversion efficiency and breast muscle mass. Objective With a view to understanding the metabolic implications of this historic selection – and also laying a foundation to better interpret current commercial performance - we quantitated the cellular bioenergetic structure of modern broilers. The breast muscle of 4 64 d old Ross308 broilers was subject to morphometric analysis based on transverse Transmission Electron Microscopy (TEM) images. Results This approach determined an average mitochondrial content, diameter and circularity ratio of 2.1%, 0.42 µm and 0.72, respectively. Broiler breast mitochondrial content has thus approximately halved in the last 45 years, and represents one of the lowest contents recorded for the muscle of any eukaryotic species. We have provided an updated benchmark for interpreting broiler muscle biochemistry and reinforce the view that domestic chickens are unique models of minimal aerobic capacity.
Glaucoma-related posts from a Chinese social media: An exploratory study
Authors: Fu Junxia , Yang Junrui , Li Qiuman , Huang Danqing , Yang Hongyang , Xie Xiaoling , Xu Huaxin , Zhang Mingzhi , Zheng Ce
Published: December 2022

Purpose: Our study aims to discuss glaucoma patients' needs and Internet habits using big data analysis and Natural Language Processing (NLP) based on deep learning (DL). We also developed and validated DL models to recognize social media data.
Methods: In this retrospective study, we used web crawler technology to crawl glaucoma-related topic posts from the glaucoma bar of Baidu Tieba. According to the contents of topic posts, we classified them into posts with or without seeking medical advice. Word Cloud and frequency statistics were used to analyze the contents and visualize the keywords. Two DL models, Bidirectional Long Short-Term Memory (Bi-LSTM) and Bidirectional Encoder Representations from Transformers (BERT), were trained to identify the posts seeking medical advice. The evaluation matrices included: accuracy, F1 value, and the area under the ROC curve (AUC).
Results: A total of 10,892 topic posts were included, among them, most were seeking medical advice (N=7071, 64.91%), and seeking advice regarding symptoms or examination (N=4913, 45.11%) dominated the majority, followed by searching for social support , expressing emotions, and sharing knowledge. The word cloud analysis showed that ocular pressure, visual field, examination, and operation were the most frequent words. The accuracy, F1 score, and AUC were 0.891, 0.891, and 0.931 for BERT model, 0.82, 0.821, and 0.890 for Bi-LSTM model.
Conclusion: Social media can help enhance the patient-doctor relationship by providing patients’ concerns and cognition about glaucoma. DL models performed well in classifying Chinese medical-related texts, which could play an important role in public health monitoring.
Experimental study on disaster mechanism of completely weathered granite landslide induced by extreme rainfall
Authors: Liu Honghua , Yu Peng , Lu Haitao , Xie Yongjian , Wang Zhongsheng , Hao Shengyou , Liu Hongjun , Fu Yunxia
Published: December 2022

With the increased frequency of extreme weather, landslides induced by extremely heavy rainfall pose a major threat to human lives and property safety. Taking the 7.23 Fanling landslide in Laoshan, Qingdao, a typical strongly weathered granite area, as an example, based on the similarity criterion, three groups of indoor model tests under different extreme rainfall conditions were conducted using a large landslide model test box. The rainfall infiltration responses under different rainfall intensities, deformation processes, and failure modes of the slope were analyzed. The inducing mechanism and rainfall disaster process of granite landslides induced by extreme rainfall in strongly weathered areas were summarized. The results showed that (1) a completely weathered granite landslide induced by rainfall had four stages, i.e., infiltration erosion, surface deformation, damage deepening, and overall instability, and the slope was characterized by "sheet slip". (2) With greater rainfall intensities, the rainfall infiltration rate was higher, the changes in soil pressure, pore water pressure, water content, and matrix suction were faster, and the hysteresis effect was weaker. (3) A certain spatial distribution pattern was observed between slope deformation/damage and rainfall infiltration, and the research results could provide references for landslide warning and treatment in strongly weathered granite areas.
Spontaneous Intracerebral Haemorrhage Secondary to 5-ALA-induced Thrombocytopaenia in a Paediatric Patient: Case Report and Literature Review
Authors: Mui Olivia , Murray Daniel B , Walsh Bill , Crimmins Darach W , Caird John D
Published: December 2022

Introduction
The primary objective of neurosurgical management of malignant gliomas is maximal safe resection of the tumour. One of the main obstacles in achieving this is the ability to accurately discriminate between tumour edges and the surrounding healthy brain tissue. The use of fluorescence-guided surgery utilising 5-aminolevulinic acid (5-ALA), first introduced more than twenty years ago, has become an invaluable adjunct in high-grade glioma surgery in adults. However, as 5-ALA is not licensed for use in paediatric patients, the safety profile for such use remains undetermined.
Case Report
We describe the case of a four year-old boy who underwent 5-ALA-guided resection of a fourth ventricle anaplastic ependymoma. Although complete resection was achieved and the patient awoke from surgery well with no neurological deficits, the patient developed acute transaminitis, anaemia, thrombocytopaenia and coagulopathy post-operatively. The patient had a sudden neurological deterioration on post-operative day two; imaging revealed that he had suffered a spontaneous right frontal intracerebral haemorrhage. The patient returned to theatre for surgical decompression and evacuation of the haematoma, and ultimately went on to make a full recovery.
Conclusion
The use of 5-ALA in paediatric patients can be helpful in maximising surgical resection, but the associated safety profile remains undefined. Further research is urgently warranted in order to characterise the efficacy and risk of the use of 5-ALA in the paediatric population.
New method for the isotopic study of ancient conchiolin from archaeological shells of freshwater mussels (Unionoida)
Authors: García-Vázquez Ana , Radu Valentin , Lazăr Cǎtǎlin
Published: December 2022

Freshwater mussels shells are common remains in archaeological sites of the Gumelnița culture (5th millennium BC, Romania), and were part of the diet of its ancient inhabitants. The proteins of these shells are often preserved and can be used for paleodietary and paleoecosystem reconstructions by stable isotopes. To obtain the empirical relationship between the isotopic composition of sell organic matrix and soft tissues, we analysed the body (foot and mantle), the muscle, the conchiolin of the shell and stomach contents of modern individuals of Unio tumidus from the Danube. In addition, modern shells were digested in 5M HCl and archaeological in 1M HCl to obtain the shell organic matrix or conchiolin. We established a quality criterion for freshwater mussel shell conchiolin of 3.6 (± 0.3) for the C:N and obtained an offset of Δ15Nconchiolin−defatted body = + 0.95‰, Δ13Cconchiolin−defatted body = + 0.93‰ for the soft tissues, and an offset of Δ15Nconchiolin−muscle = + 1.7‰, Δ13Cconchiolin−muscle = + 0.3‰ for the muscle. Freshwater mussels from Gumelnița showed that they came from different sources (rivers, lakes, ponds…) but which not necessarily have to come from long distances since all these different habitats were present in the vicinity of the site.
Emoji-based Opinion Mining using Deep Learning Techniques
Authors: Pathan Azizkhan F , Prakash Chetana
Published: December 2022

Informal media, such as Twitter, are more relevant today than ever before. Twitter is still a valuable tool for friends to communicate, but it has evolved into a public bulletin board where ordinary people, companies, and even big personalities like politicians and sports routinely publish their thoughts and participate in conversations. Since, Twitter is so extensively utilized throughout the world, the ability to do reliable opinion mining and gauge public opinion and perception on a variety of issues is more vital than ever. Emojis are frequently used to communicate feelings or sentiments that are difficult to express succinctly in language. For emoji-based opinion mining, a deep learning framework is presented to model the influence of emojis on text sentiment polarity. The emojis and words in microblog posts are combined to create emoji representations that include contextual information.
Embolic microspheres for transarterial chemo-embolization (TACE) featuring inherent radiopacity and carrying crystalline drugs at their surface, in dimples or inside major cavities
Authors: Koole Leo H. , Shen Meitong
Published: December 2022

Transarterial chemoembolization (TACE) using drug-eluting embolic (DEE) microparticles is a minimally invasive treatment technique that finds more and more applications in oncology, particularly in the treatment of patients with hepatocellular carcinoma. The last decade has seen marked developments in TACE, mainly due to innovations regarding the embolic microparticles. We studied a new versatile approach for charging TACE embolic particles (3D-crosslinkled polymer microspheres) with crystalline (cytostatic) drugs. The crystals are physically attached to the microporous particles. This approach provides a promising alternative for the ion-exchange regime that underlies the mechanism of action of all TACE/DEE products which are currently available commercially. Moreover, chemical synthesis is reported of new nearly spherical polymeric microparticles which either have a cavity or one or more dimples in their surface. These particles are remarkable since (i), the cavity or dimples can accommodate relatively large drug crystals in a stable manner, and (ii), their size is 1–2 orders of magnitude larger than similar shapes known in the literature. The large diameter (ranging from 200 to 600 µm roughly) renders the new particles suitable for TACE/DEE. It is argued and underpinned that the use of crystalline drug formulations according to this methodology can open new possibilities for TACE in the treatment of different solid cancers, particularly since (i), larger drug payloads can be achieved, and (ii), new options emerge to use lipophilic crystalline drugs which have been incompatible with TACE hitherto. Since many potent cytostatic medicines fall into this category, the new technique can likely become scope-widening for the TACE technique.