bluuebunny/crossref_metadata_2025 huggingface dataset:
Techno-Economic Comparison of Utility-Scale Compressed Air and Electro-Chemical Storage Systems
Authors: Salvini Coriolano , Giovannelli Ambra
Published: September 2022

The paper deals with a techno-economic comparison between utility-scale diabatic compressed air energy storage (D-CAES) systems equipped with artificial storage and Battery Energy Storage (BES) systems based on consolidated technologies, such as Sodium-Sulfur (Na-S) and Lithium-ion (Li-Ion). The comparison is carried out on the basis of the levelized cost of storage (LCOS). Analyses have been performed by varying key inputs, such as the rated power, the storage capacity, the price of electricity absorbed from the grid during the charging phase, and the cost of fuel fed to D-CAES during the discharge phase. Na-S technology-based systems always show better techno-economic performance in respect to Li-Ion based ones. The economic performance of both D-CAES and BES improves by increasing the storage capacity. The D-CAES performance improvement rate, however, is higher than that estimated for BES based systems. Moreover, the economic performance of D-CAES systems is less sensitive to the price of electricity in respect of BES based storage facilities. As a result, D-CAES based solutions can reach a LCOS lower than that of Na-S batteries if the size of the system and the price of electricity are large enough.
Classification of Single Current Sensor Failures in Fault-Tolerant Induction Motor Drive Using Neural Network Approach
Authors: Skowron Maciej , Teler Krystian , Adamczyk Michal , Orlowska-Kowalska Teresa
Published: September 2022

In the modern induction motor (IM) drive system, the fault-tolerant control (FTC) solution is becoming more and more popular. This approach significantly increases the security of the system. To choose the best control strategy, fault detection (FD) and fault classification (FC) methods are required. Current sensors (CS) are one of the measuring devices that can be damaged, which in the case of the drive system with IM precludes the correct operation of vector control structures. Due to the need to ensure current feedback and the operation of flux estimators, it is necessary to immediately compensate for the detected damage and classify its type. In the case of the IM drives, there are individual suggestions regarding methods of classifying the type of CS damage during drive operation. This article proposes the use of the classical multilayer perceptron (MLP) neural network to implement the CS neural fault classifier. The online work of this classifier was coordinated with the active FTC structure, which contained an algorithm for the detection and compensation of failure of one of the two CSs used in the rotor field-oriented control (DRFOC) structure. This article describes this structure and the method of designing the neural fault classifier (NN-FC). The operation of the NN-FC was verified by simulation tests of the drive system with an integrated FTC strategy. These tests showed the high efficiency of the developed fault classifier operating in the post-fault mode after compensating the previously detected CS fault and ensuring uninterrupted operation of the drive system.
A Novel Magnetic Circuit Design Method for a Permanent Magnetic Chuck of a Wall-Climbing Robot
Authors: Zhang Yulong , Guan Enguang , Li Peixing , Zhao Yanzheng
Published: September 2022

Permanent magnet wall-climbing robots are widely used in the maintenance of tanks in the petrochemical industry and the overhaul of large-pressure pipelines in the hydropower industry. One of the difficulties is to achieve the safe and reliable adsorption of wall-climbing robots. Based on the Halbach array, a double-layer superposition magnetic circuit magnetization method is designed in this paper. Under the same constraints, the adsorption force of the permanent magnetic chuck is increased by at least 8% compared with the traditional magnetic circuit design method. Under the working air gap of 1∼9 mm, the average magnetic energy utilization rate is increased by at least 16.46%. This approach not only improves the magnetic energy utilization of the permanent magnetic chuck but also improves the adsorption safety of the wall-climbing robot.
The Weighted Average Cost of Capital and Its Universality in Crisis Times: Evidence from the Energy Sector
Authors: Dobrowolski Zbysław , Drozdowski Grzegorz , Panait Mirela , Apostu Simona Andreea
Published: September 2022

Recent economic anomalies, including the unprecedented lockdown generated by the COVID-19 crisis, have demonstrated that the weighted average cost of capital (WACC) remains an actual topic in the financial literature and in practice. Companies operate in an increasingly volatile environment, due to twin transitions and interlinked crises, and so they must have specific tools for measuring risk and profitability, in order to enable them to have a sound financial policy. Based on the earlier results obtained by Modigliani and Miller (1963), Harris and Pringle (1985), and Farber, Gillet, and Szafarz (2006), this study shows the relationship between WACC and interest rate. It offers a modified WACC formula that considers unstable market circumstances. The new redefined WACC can be a valuable tool in business planning for companies from different fields. The companies in the energy sector are very interested in the topic of WACC, considering not only the complex nature of the investments made and the long-term nature of investment recovery but also the multiple risks that have an impact on their activity and that can be found in different economic, social, and geopolitical spheres.
Deviation from Darcy Law in Porous Media Due to Reverse Osmosis: Pore-Scale Approach
Authors: Dorhjie Desmond Batsa , Yusupov Roman , Krutko Vladislav , Cheremisin Alexey
Published: September 2022

Shale and tight hydrocarbons are vital to global energy dynamics. The fluid flow in sub-micron pores of tight oil reservoirs varies from bulk fluid flow. The Darcy law is widely accepted to model creeping flow in petroleum reservoirs. However, traditional reservoir modeling approaches fail to account for the sub-micron mechanisms that govern fluid flow. The accuracy of tight oil reservoir simulators has been improved by incorporating the influence of sub-micron effects. However, there are still factors that affect sub-micron fluid mobility that need investigation. The influence of a chemical potential gradient on fluid flow in sub-micron pores was modeled by solving Darcy and the transport and diluted species equations. The findings indicate that when a chemical potential gradient acts in the opposite direction of a hydraulic pressure gradient (reverse osmosis), there exists a limiting pressure threshold below which a non-linear flow pattern deviating from the Darcy equation is observed. Furthermore, the simulation based on tight reservoir pore parameters shows that when the effect of a chemical potential gradient is added, the resultant flux is 8–49% less. Hence, including the effect of the chemical potential gradient will improve the accuracy of sub-micron pressure dynamics and flow velocity.
New Insights into Biomass and Biofuels in Rapidly Changing Energy Scenario
Authors: Rosillo-Calle Francisco
Published: September 2022

The energy sector is going through considerable upheavals due to a combination of factors such as climate change, environmental pressure, the need for urgent decarbonization, and the Russia–Ukraine war [...]
The Psychosocial Risk Factors Evaluation and Management of Shift Personnel at Forest Harvesting
Authors: Korneeva Yana , Simonova Natalia , Shadrina Nina
Published: September 2022

The study describes psychosocial risk factors at work in relation to the general functional state of a body, working capacity and stress among shift workers at a logging enterprise in the Far North. The study involved 153 loggers with a 14-day shift period. Research methods included the psychophysiological instrumental method (variocardiointervalometry) used to assess the general functional state of a body, M. Luscher’s color test to assess working capacity and stress and QNordic to assess psychosocial factors. Statistical analysis was performed using multiple stepwise regression analysis and frequency analysis. It was found that 71.1% of employees have a favorable general functional state of the body, 28.9%—unfavorable; the forest loggers who took part in the survey have a high level of working capacity and a low level of stress. It was substantiated that the general functional state of a body, stress and working capacity, measured by objective and subjective methods, are differentially interconnected with psychosocial factors at work. The general functional state of forest harvesting workers is influenced by factors of labor content, intensity and organization. The relationships with the immediate supervisor are important in order to increase the working capacity and efficiency of employees as well as their involvement in work.
MTL-FFDET: A Multi-Task Learning-Based Model for Forest Fire Detection
Authors: Lu Kangjie , Huang Jingwen , Li Junhui , Zhou Jiashun , Chen Xianliang , Liu Yunfei
Published: September 2022

Deep learning-based forest fire vision monitoring methods have developed rapidly and are becoming mainstream. The existing methods, however, are based on enormous amounts of data, and have issues with weak feature extraction, poor small target recognition and many missed and false detections in complex forest scenes. In order to solve these problems, we proposed a multi-task learning-based forest fire detection model (MTL-FFDet), which contains three tasks (the detection task, the segmentation task and the classification task) and shares the feature extraction module. In addition, to improve detection accuracy and decrease missed and false detections, we proposed the joint multi-task non-maximum suppression (NMS) processing algorithm that fully utilizes the advantages of each task. Furthermore, considering the objective fact that divided flame targets in an image are still flame targets, our proposed data augmentation strategy of a diagonal swap of random origin is a good remedy for the poor detection effect caused by small fire targets. Experiments showed that our model outperforms YOLOv5-s in terms of mAP (mean average precision) by 3.2%, APS (average precision for small objects) by 4.8%, ARS (average recall for small objects) by 4.0%, and other metrics by 1% to 2%. Finally, the visualization analysis showed that our multi-task model can focus on the target region better than the single-task model during feature extraction, with superior extraction ability.
Knowledge about Plant Coexistence during Vegetation Succession for Forest Management on the Loess Plateau, China
Authors: Tian Qilong , Zhang Xiaoping , Xu Xiaoming , Yi Haijie , He Jie , He Liang , Sun Weinan
Published: September 2022

Coexistence between species within plant communities is a key issue in the practice of revegetation, forest management, and biodiversity conservation. Vegetation restoration is critical to control soil erosion and improve the ecological environment on the Loess Plateau. Here, we investigate the interspecific relationships of dominant plants during natural vegetation succession on the Loess Plateau. The results suggest that, under the ecological process of environmental filtering, species within communities can reduce interspecific competition and promote species coexistence via spatial heterogeneity and temporal asynchronous differences. The ecological niche overlap index (Oik) significantly and positively correlated with the strength of interspecific associations. Most species pairs had weak competition and more stable interspecific relationships. The results of the χ2 test showed that 317 species pairs were positively associated and 118 were negatively associated. The community is in a positive succession process, and the interaction relationship between species tends to be neutral. We should enhance the protection of positively associated species and pay attention to negatively associated species during forest management. Results revealed that Carex lanceolata Boott and Lespedeza bicolor Turcz coexisted easily with other species for mutual benefit, which could help build artificial forestland of native species to improve the ecological function.
Mapping Secondary Vegetation of a Region of Deforestation Hotspot in the Brazilian Amazon: Performance Analysis of C- and L-Band SAR Data Acquired in the Rainy Season
Authors: Kiyohara Bárbara Hass , Sano Edson Eyji
Published: September 2022

The re-suppression of secondary vegetation (SV) in the Brazilian Amazon for agriculture or land speculation occurs mostly in the rainy season. The use of optical images to monitor such re-suppression during the rainy season is limited because of the persistent cloud cover. This study aimed to evaluate the potential of C- and L-band SAR data acquired in the rainy season to discriminate SV in an area of new hotspot of deforestation in the municipality of Colniza, northwestern of Mato Grosso State, Brazil. This is the first time that the potential of dual-frequency SAR data was analyzed to discriminate SV, with an emphasis on data acquired during the rainy season. The L-band ALOS/PALSAR-2 and the C-band Sentinel-1 data acquired in March 2018 were processed to obtain backscattering coefficients and nine textural attributes were derived from the gray level co-occurrence matrix method (GLCM). Then, we classified the images based on the non-parametric Random Forest (RF) and Support Vector Machine (SVM) algorithms. The use of SAR textural attributes improved the discrimination capability of different LULC classes found in the study area. The results showed the best performance of ALOS/PALSAR-2 data classified by the RF algorithm to discriminate the following representative land use and land cover classes of the study area: primary forest, secondary forest, shrubby pasture, clean pasture, and bare soil, with an overall accuracy and Kappa coefficient of 84% and 0.78, respectively. The RF outperformed the SVM classifier to discriminate these five LULC classes in 14% of overall accuracy for both ALOS-2 and Sentinel-1 data sets. This study also showed that the textural attributes derived from the GLCM method are highly sensitive to the moving window size to be applied to the GLCM method. The results of this study can assist the future development of an operation system based on dual-frequency SAR data to monitor re-suppression of SV in the Brazilian Amazon or in other tropical rainforests.
Effects of Tourism Trampling on Soil Nitrogen Mineralization in Quercus variabilis Blume Forests Varies with Altitudes in the Climate Transition Zone
Authors: Shang Qing , Liu Yanchun , Li Qinglin
Published: September 2022

Tourism trampling is one of the critical disturbance factors affecting forest structure and function apart from forest management activities. However, how tourism trampling affects soil nitrogen (N) mineralization rate at different altitudes in scenic forest spots is still unclear. To determine whether the responses of soil net N mineralization rate to tourism trampling varies with altitudes, we incubated soils using a field buried pipe method and analyzed soil ammonium N (NH4+-N) and nitrate N (NO3−-N) content at three altitudes (810 m, 1030 m, and 1240 m) at the Baotianman forest scenic spot in Henan Province. The results showed that tourism trampling significantly increased the soil bulk density and soil pH value but substantially reduced soil organic carbon (C) and total N content at all altitudes. Tourism trampling also resulted in a significant decrease in NO3−-N in the soil before and after incubation at all altitudes. The effects of tourism trampling on soil net N mineralization varied with latitudes, showing positive effects at 1030 m altitude (+51.4%), but negative effects at 1240 m altitude (−43.5%). For the soil net N nitrification rate, however, tourism trampling resulted in an increased rate (+141.1%) only at the 810 m altitude. Across all altitudes, soil microbial biomass C is primarily responsible for the variation in the soil net N mineralization rate. This study indicates that the effect of tourism trampling on soil net N mineralization rate varies with altitudes, which is related to the intensity of tourist disturbance and the synthetic effects of vegetation and soil microbes.
Optimization of Growth Conditions to Enhance PHA Production by Cupriavidus necator
Authors: Ronďošová Soňa , Legerská Barbora , Chmelová Daniela , Ondrejovič Miroslav , Miertuš Stanislav
Published: September 2022

The accumulation of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress conditions. Therefore, it is advantageous to choose two-step cultivation. The first phase is aimed at maximizing biomass production, and only in the second phase, after setting the suitable conditions, PHA production starts. The aim of this work was to optimize the composition of the minimal propagation medium used for biomass production of Cupriavidus necator DSM 545 using the response surface methodology (RSM). Based on the results from the search for optimization limits, the glucose concentration, the ammonium sulfate concentration and the phosphate buffer molarity were chosen as independent variables. The optimal values were found as follows: the glucose concentration 10.8 g/L; the ammonium sulfate concentration 0.95 g/L; and the phosphate buffer molarity 60.2 mmol/L. The predicted biomass concentration was 4.54 g/L, and the verified value was at 4.84 g/L. Although this work was primarily focused on determining the optimal composition of the propagation medium, we also evaluated the optimal composition of the production medium and found that the optimal glucose concentration was 6.7 g/L; the ammonium sulfate concentration 0.60 g/L; and the phosphate buffer molarity 20 mmol/L. The predicted PHB yield was 54.7% (w/w) of dry biomass, and the verified value was 49.1%.
Effects of Lactobacillus curvatus HY7602-Fermented Antlers in Dexamethasone-Induced Muscle Atrophy
Authors: Jeon Hyejin , Kim Yong-Tae , Jang Woo Young , Kim Joo-Yun , Heo Keon , Shim Jae-Jung , Lee Jung-Lyoul , Yang Deok-Chun , Kang Se Chan
Published: September 2022

This study assessed the improvements yielded by Lactobacillus curvatus HY7602-fermented antlers (FA) in dexamethasone-induced muscle atrophy and the effects of bioactive compounds increased by fermentation. Dexamethasone-treated C2C12 myoblast cells were treated with FA and non-fermented antlers (NFA). FA showed inhibitory effects on muscle protein degradation in the C2C12 cells. Hsb:ICR mice were orally administered saline (control(CON) and dexamethasone only (DEX)), oxymetholone (DEX+OXY), NFA (DEX+NFA), and FA (DEX+FA) via gavage. Before the end of the experiment, dexamethasone was intraperitoneally (IP) injected into the mice, except in the control group, to induce muscle atrophy. Compared with the DEX group, the DEX+FA group exhibited a significant prevention in the reduction of hindlimb strength, calf thickness, calf muscle weight, and the cross-sectional area of muscle fibers (p < 0.05). The FA-induced improvements in muscle atrophy were associated with a decreased gene expression of protein degradation and growth inhibition, and an increased gene expression of protein synthesis and growth factors. Sialic acid, a bioactive compound associated with muscles, was increased by 51.41% after fermentation and suppressed the expression of protein degradation genes in the C2C12 cells. L. curvatus HY7602-fermented antlers with increased sialic acid after fermentation may therefore be useful for preventing and improving muscle atrophy.
Choice between IEO and ICO: Speed vs. Liquidity vs. Risk
Authors: Miglo Anton
Published: September 2022

This paper analyzes a financing problem for an innovative firm that is considering launching a web-based platform. The model developed in the paper is the first one that analyzes an entrepreneur’s choice between initial exchange offering (IEO) and initial coin offering (ICO). Compared to ICO, under IEO the firm is subject to screening by an exchange that reduces the risk of investment in tokens; also the firm receives access to a larger set of potential investors; finally tokens become listed on an exchange faster. The paper argues that IEO is a better option for the firm if: (1) the investment size is relatively large; (2) the extent of moral hazard problems faced by the firm is relatively large; (3) the degree of investors’ impatience is relatively small. Furthermore, a non-linear relationship between firm quality and its financing choice is found. Most of these predictions are new and have not been tested so far.
Analyzing the Synchronous Acoustic and Electric Response of Coal Burst Failure: Validation through the On-Site Application
Authors: Zhao Yingjie , Song Dazhao , Wei Menghan , Khan Majid , Li Zhenlei , Qiu Liming , Yin Shan
Published: September 2022

The accurate monitoring and early warning of coal and rock dynamic disasters become challenging in complex geological environments. Mostly, the signal information contains interferences, which misguides the technician, and thus leads to inaccurate monitoring results. To reduce the influence of interference signals, the synchronous response of the acoustic emission (AE) and electromagnetic emission (EME) signals before the failure of coal specimens during uniaxial loading was investigated in this study. Additionally, the coupling relationship between M value, AE energy/AE ringing count per unit time, and the damage of coal is established, and the early warning index of AE and EME (R value) was computed and verified through the field investigations. The results show that a strong synchronization of the acoustoelectric signals occurs only after the specimen enters the strain strengthening area. The analysis of the obtained results showed that the M value of the AE-EME synchronous response signal represents a strong degree of damage occurring in the coal body, however, this is still subject to false alarms. In contrast, the analysis of the R value accurately helped in determining the damage evaluation, thus, it can be regarded as one of the precursors of the imminent failure of coal. With R > 1, the specimen is closed to the failure state, thereby dangerous regions are identified with a dense concentration of R > 1 events. The obtained R value index through on-site AE and EME monitoring corresponds closely with the stress distribution cloud map of the roadway. It is inferred that the anti-interference ability and the reliability of the R value index are stronger than the routine early warning indicators of the single-AE or EME energy.
Point Shear Wave Elastography and 2-Dimensional Shear Wave Elastography as a Non-Invasive Method in Differentiating Benign from Malignant Liver Lesions
Authors: Nacheva-Georgieva Emiliya Lyubomirova , Doykov Daniel Ilianov , Andonov Vladimir Nikolov , Doykova Katya Angelova , Tsvetkova Silviya Bogdanova
Published: September 2022

Non-invasive, ultrasound-based methods for visualizing and measuring tissue elasticity are becoming more and more common in routine daily practice. An accurate diagnosis of malignant and benign tumors is essential for determining the appropriate treatment. Despite the wide use of imaging techniques, the investigation for assessing the elasticity of focal liver lesions and their differentiating is still continuing. Aim: To investigate the value of point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) for the differential diagnosis of benign and malignant focal liver lesions. Materials and Methods: A total of 125 adult patients were included from the Clinic of Gastroenterology of University Hospital Kaspela, Plovdiv city, Bulgaria, in the period from January 2021 to July 2022. Participants were divided into two groups—with benign (hemangiomas) and malignant focal liver lesions (hepatocellular carcinoma). The group with benign lesions included 63 patients and the group with malignant focal liver lesions (FLLs)—62 patients. Point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) integrated in the same ultrasound machine (Esaote MyLab™ 9Exp) were performed for each lesion. Results: Malignant FLLs have significantly higher stiffness in both pSWE (2.52–4.32 m/s, 90% CI: 2.37 to 2.68, 90% CI: 4.19 to 4.55) and 2d-SWE (2.52–4.43 m/s, 90% CI: 2.31 to 2.65, 90% CI: 4.27 to 4.61). Conclusion: 2D-SWE and pSWE could provide complementary data about FLLs. They enable us to conveniently and easily obtain accurate stiffness information of FLLs.
Twenty-Five Years of Contemplating Genotype-Based Hereditary Hemochromatosis Population Screening
Authors: Schmidtke Jörg
Published: September 2022

Hereditary hemochromatosis (HH) is a rather frequent, preventable disease because the progressive iron overload affecting many organs can be effectively reduced by phlebotomy. Even before the discovery of the major gene, HFE, in 1996, hemochromatosis was seen as a candidate for population-wide screening programmes. A US Centers of Disease Control and the National Human Genome Research Institute expert panel convened in 1997 to consider genotype-based HH population-wide screening and decided that the scientific evidence available at that time was insufficient and advised against. In spite of a large number of studies performed within the last 25 years, addressing all aspects of HH natural history, health economics, and social acceptability, no professional body worldwide has reverted this decision, and HH remains a life-threatening condition that often goes undetected at a curable stage.
Prenatal Diagnosis and Outcomes in Fetuses with Hemivertebra
Authors: Zhou Hang , Wang You , Huang Ruibin , Fu Fang , Li Ru , Cheng Ken , Wang Dan , Yu Qiuxia , Zhang Yongling , Jing Xiangyi , Lei Tingying , Han Jin , Yang Xin , Li Dongzhi , Liao Can
Published: September 2022

Background: There are few studies on the burden of chromosomal abnormalities and single gene disorders in fetal hemivertebra (HV). We aim to investigate the cytogenetic and monogenic risk and evaluate prenatal outcomes of fetal HV. Method: This study included fetuses diagnosed with HV divided into two groups: isolated HV and non-isolated HV. Data on other sonographic structural anomalies, chromosomal and sub-chromosomal abnormalities, monogenic variations detected by WES, and prenatal outcomes are recorded and reviewed. Results: Among 109 fetal HV cases, forty-seven (43.1%) non-isolated HV cases were associated with structural anomalies. Chromosomal test results were available in 58 cases, identifying six (10.3%) chromosomal aberrations involved in four isolated and two non-isolated HV. WES identified four (likely) pathogenic variants in three cases among 16 fetuses with HV, involving three novel variants, 1250G > T and c.1277G> inherited from parents, respectively, in DLL3 and c.7213C > A ** in the FLNB. The live birth rate (LB) was higher in the isolated fetal HV group than in the non-isolated group (67.7% (42/62) vs. 12.5% (12/47), p < 0.001). Conclusion: This study emphasizes the risk of cytogenetic abnormalities in isolated HV. WES yields a diagnostic rate of 18.3% in HV with normal CMA, probably aiding the prenatal counseling and management of fetal HV.
Whole-Genome Sequencing and Comparative Genomics Analysis of the Wild Edible Mushroom (Gomphus purpuraceus) Provide Insights into Its Potential Food Application and Artificial Domestication
Authors: Geng Yangyang , Zhang Shixin , Yang Ningxian , Qin Likang
Published: September 2022

Gomphus purpuraceus (Iwade) Yokoyama is a species of wild fungi that grows in southwest China, considered an edible and medicinal fungus with potential commercial prospects. However, the detailed mechanisms related to the development of mycelium and the formation of the fruiting body are unclear. To obtain a comprehensive overview of genetic features, whole-genome and comparative genomics analyses of G. purpuraceus were performed. High-quality DNA was extracted from the mycelium, which was isolated from a fresh fruiting body of G. purpuraceus. The DNA sample was subjected to sequencing using Illumina and Oxford Nanopore sequencing platforms. A genome assembly totaling 40.15 Mb in 50 contigs with an N50 length of 2.06 Mb was generated, and 8705 putative predicted genes were found. Subsequently, phylogenetic analysis revealed a close evolutionary relationship between G. purpuraceus and Gomphus bonarii. Moreover, a total of 403 carbohydrate-active enzymes (CAZymes) were identified in G. purpuraceus, which included 147 glycoside hydrolases (GHs), 85 glycosyl transferases (GTs), 8 polysaccharide lyases (PLs), 76 carbohydrate esterases (CEs), 57 auxiliary activities (AAs) and 30 carbohydrate-binding modules (CBMs). Compared with the other 13 fungi (Laccaria bicolor, Russula virescens, Boletus edulis, etc.), the number and distribution of CAZymes in G. purpuraceus were similar to other mycorrhizal fungi. Furthermore, the optimization of culture medium for G. purpuraceus showed the efficient utilization of disaccharides such as sucrose and maltose. The genome of G. purpuraceus provides new insights into its niche, food applications and potential artificial domestication.
Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
Authors: Harpke Marie , Pietschmann Sebastian , Ueberschaar Nico , Krüger Thomas , Kniemeyer Olaf , Brakhage Axel A. , Nietzsche Sandor , Kothe Erika
Published: September 2022

Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones.
GWAS and Transcriptome Analysis Reveal Key Genes Affecting Root Growth under Low Nitrogen Supply in Maize
Authors: Wang Yunyun , Zhu Tianze , Yang Jiyuan , Wang Houmiao , Ji Weidong , Xu Yang , Yang Zefeng , Xu Chenwu , Li Pengcheng
Published: September 2022

Nitrogen (N) is one of the most important factors affecting crop production. Root morphology exhibits a high degree of plasticity to nitrogen deficiency. However, the mechanisms underlying the root foraging response under low-N conditions remain poorly understood. In this study, we analyzed 213 maize inbred lines using hydroponic systems and regarding their natural variations in 22 root traits and 6 shoot traits under normal (2 mM nitrate) and low-N (0 mM nitrate) conditions. Substantial phenotypic variations were detected for all traits. N deficiency increased the root length and decreased the root diameter and shoot related traits. A total of 297 significant marker-trait associations were identified by a genome-wide association study involving different N levels and the N response value. A total of 51 candidate genes with amino acid variations in coding regions or differentially expressed under low nitrogen conditions were identified. Furthermore, a candidate gene ZmNAC36 was resequenced in all tested lines. A total of 38 single nucleotide polymorphisms and 12 insertions and deletions were significantly associated with lateral root length of primary root, primary root length between 0 and 0.5 mm in diameter, primary root surface area, and total length of primary root under a low-N condition. These findings help us to improve our understanding of the genetic mechanism of root plasticity to N deficiency, and the identified loci and candidate genes will be useful for the genetic improvement of maize tolerance cultivars to N deficiency.
Biallelic Loss of Function Mutation in Sodium Channel Gene SCN10A in an Autism Spectrum Disorder Trio from Pakistan
Authors: Rabia Ansa , Harripaul Ricardo , Mikhailov Anna , Mahmood Saqib , Maqbool Shazia , Vincent John B. , Ayub Muhammad
Published: September 2022

The genetic dissection of autism spectrum disorders (ASD) has uncovered the contribution of de novo mutations in many single genes as well as de novo copy number variants. More recent work also suggests a strong contribution from recessively inherited variants, particularly in populations in which consanguineous marriages are common. What is also becoming more apparent is the degree of pleiotropy, whereby mutations in the same gene may have quite different phenotypic and clinical consequences. We performed whole exome sequencing in a group of 115 trios from countries with a high level of consanguineous marriages. In this paper we report genetic and clinical findings on a proband with ASD, who inherited a biallelic truncating pathogenic/likely pathogenic variant in the gene encoding voltage-gated sodium channel X alpha subunit, SCN10A (NM_006514.2:c.937G>T:(p.Gly313*)). The biallelic pathogenic/likely pathogenic variant in this study have different clinical features than heterozygous mutations in the same gene. The study of consanguineous families for autism spectrum disorder is highly valuable.
Association of the ACTN3 rs1815739 Polymorphism with Physical Performance and Injury Incidence in Professional Women Football Players
Authors: Del Coso Juan , Rodas Gil , Buil Miguel Ángel , Sánchez-Sánchez Javier , López Pedro , González-Ródenas Joaquín , Gasulla-Anglés Pablo , López-Samanes Álvaro , Hernández-Sánchez Sergio , Iztueta Ane , Moreno-Pérez Víctor
Published: September 2022

The p.R577X polymorphism (rs1815739) in the ACTN3 gene causes individuals with the XX genotype to be deficient in functional α-actinin-3. Previous investigations have found that XX athletes are more prone to suffer non-contact muscle injuries, in comparison with RR and RX athletes who produce a functional α-actinin-3 in their fast-twitch fibers. This investigation aimed to determine the influence of the ACTN3 R577X polymorphism on physical performance and injury incidence of players competing in the women’s Spanish first division of football (soccer). Using a cross-sectional experiment, football-specific performance and epidemiology of non-contact football-related injuries were recorded in a group of 191 professional football players. ACTN3 R577X genotype was obtained for each player using genomic DNA samples obtained through buccal swabs. A battery of physical tests, including a countermovement jump, a 20 m sprint test, the sit-and-reach test and ankle dorsiflexion, were performed during the preseason. Injury incidence and characteristics of non-contact injuries were obtained according to the International Olympic Committee (IOC) statement for one season. From the study sample, 28.3% of players had the RR genotype, 52.9% had the RX genotype, and 18.8% had the XX genotype. Differences among genotypes were identified with one-way analysis of variance (numerical variables) or chi-square tests (categorical variables). Jump height (p = 0.087), sprint time (p = 0.210), sit-and-reach distance (p = 0.361), and dorsiflexion in the right (p = 0.550) and left ankle (p = 0.992) were similar in RR, RX, and XX football players. A total of 356 non-contact injuries were recorded in 144 football players while the remaining 47 did not sustain any non-contact injuries during the season. Injury incidence was 10.4 ± 8.6, 8.2 ± 5.7, and 8.9 ± 5.3 injuries per/1000 h of football exposure, without differences among genotypes (p = 0.222). Injury rates during training (from 3.6 ± 3.7 to 4.8 ± 2.1 injuries per/1000 h of training exposure, p = 0.100) and match (from 47.8 ± 9.5 to 54.1 ± 6.3 injuries per/1000 h of match exposure, p = 0.209) were also similar in RR, RX, and XX football players. The ACTN3 genotype did not affect the mode of onset, the time needed to return to play, the type of injury, or the distribution of body locations of the injuries. In summary, women football players with different genotypes of the p.R577X ACTN3 polymorphism had similar values of football-specific performance and injury incidence. From a practical perspective, the ACTN3 genotyping may not be useful to predict performance or injury incidence in professional women football players.
“Miles Is a Mode; Coltrane Is, Power”: Notes on John Coltrane as Poetic Muse and Michael Harper’s “Alone” in Songlines in Michaeltree (2000)
Authors: Mellis James
Published: September 2022

This article looks at the ways jazz legend John Coltrane was a muse for many Black Arts era poets and proceeds to discuss how Michael Harper rendered Coltrane in his work, focusing on editorial changes between the 1970 and 2000 versions of Michael Harper’s poem, “Alone”. In it, the author argues that the change marks a revision of the centrality of Coltrane as Harper’s muse from his early to later career.
‘Speculative Slipstreaming’: The Impact of Literary Interventions within Contemporary Science Fiction
Authors: Devanny Laura-Jane
Published: September 2022

Margaret Atwood and Jeanette Winterson are two canonical writers participating in a ‘literary slipstream’ through their ventures into science fiction, creating crossover texts that confuse the boundaries between the literary and the popular. This interface is exemplified through the awards received by these writers, which help to bring literary credibility and integrity to the genre. Atwood’s first speculative novel, The Handmaid’s Tale (1985), went on to win the Arthur C. Clarke Award and was nominated for a Nebula award and the Booker Prize, whilst her MaddAddam trilogy (2003–2015) was followed by the Arthur C. Clarke Award for Imagination in Service to Society in 2015. Winterson was awarded an OBE for her services to literature in the same year that she published The Stone Gods (2006), whilst her most recent novel Frankisstein (2019) was longlisted for the Booker Prize. This article explores the extent to which distinctions between the popular and the literary are reliant upon notions of inferiority and superiority, and the problematics of a desire to frame genre fiction according to perceived notions of literary value.
Carbon Footprint Research Based on Input–Output Model—A Global Scientometric Visualization Analysis
Authors: Han Jingwei , Tan Zhixiong , Chen Maozhi , Zhao Liang , Yang Ling , Chen Siying
Published: September 2022

Reducing the effect of mankind’s activities on the climate and improving adaptability to global warming have become urgent matters. The carbon footprint (CF), derived from the concept of ecological footprint, has been used to assess the threat of climate change in recent years. As a “top to bottom” method, input–output analysis (IOA) has become a universally applicable CF assessment tool for tracing the carbon footprint embodied in economic activities. A wide range of CF studies from the perspective of the IOA model have been presented and have made great progress. It is crucial to have a better understanding of what the relevant research focuses on in this field, yet so far a systematic synopsis of the literature is missing. The purpose of this paper is to explore the knowledge structure and frontier trends in respect of the IOA model applied to CF research using scientometric visualization analysis. The main findings of this paper are as follows. (1) Published articles show a two-stage increase in the period 2008 to 2021, and present a complex academic network of countries, authors, and institutions in this important domain. (2) The classic studies are mainly divided into three categories: literature reviews, database application introduction, and CF accounting in different scales. (3) The research hotspots and trends show that the research scales tend to be more microscopic and applications of models tend to be more detailed. In addition, supply-chain analysis and driver-factor analysis will probably become the main research directions in the future.
Digitalisation in Craft Enterprises: Perceived Technostress, Readiness for Prevention and Countermeasures—A Qualitative Study
Authors: Scheepers Louisa , Angerer Peter , Dragano Nico
Published: September 2022

Introduction: Digital technologies are increasingly used in the craft sector. Innovative technologies have several benefits for businesses but working with them can also induce negative feelings and stress. Data are lacking on perceived stress as well as the resulting strain and effects on health. However, information is needed about the principles of healthy working conditions in the context of digitalisation in the craft sector. To identify targeted starting points for preventive interventions to reduce technostress, we studied the perception of managers and employees of craft enterprises about digitalisation. Method: 26 guideline-based interviews were conducted with managers and employees in the craft sector to assess their understanding of digitalisation and the perceived effects on their health. The data analysis was based on a structured qualitative content analysis. Results: In the administrative area, digitalisation is perceived as simplifying work, as information, for example, becomes more easily accessible. The actual craft work, however, is perceived as more psychologically stressful, e.g., due to technologically more complex heating systems. Likewise, an increased stress experience is described in connection with constant accessibility and workplace surveillance. To reduce the stress experience, clear prevention wishes such as digital breaks or more participation in decisions about digital technologies are stated. Conclusion: Managers and employees in craft enterprises experience increasing psychological strain due to technostress. However, there is a willingness to take preventive interventions and a desire for more support in creating healthy working conditions in the context of digitalisation.
Economic Analysis of Patient’s Own Medication, Unit-Use and Ward Stock Utilization: Results of the First Pilot Study
Authors: Abdul Nasir Hamimatul Hayat , Goh Hui Poh , Wee Daniel Vui Teck , Goh Khang Wen , Lee Kah Seng , Hermansyah Andi , Al-Worafi Yaser Mohammed , Ming Long Chiau
Published: September 2022

Background: Medication wastage is causing a cost burden to the healthcare system that is worth millions of dollars. An economic and ecological friendly intervention such as using a patient’s own medications (POM) has proven to reduce wastage and save the cost spent by the hospital. The potential benefits of using POM in inpatient settings have yet to be explored in a country with universal health coverage. This study aimed to pilot test the POM intervention in an adult ward setting and to perform the economic analysis of using POM and ward stock during hospitalization. Methods: A prospective cross-sectional observational study was conducted among the patients admitted to the medical and surgical wards in a public hospital located in Brunei Darussalam between February 2022 and April 2022. Hospitalized adults above 18 years old with regular medications with a minimum length of stay of 48 h and a maximum length of stay of 21 days were included in the study. These eligible patients were divided into a POM group and a non-POM group. The economic analysis of using POM was performed by calculating the direct cost per unit of medication used during admission (from unit-use, ward stock and POM) and comparing the cost spent for both groups. Expired ward stock deemed as medication wastage was determined. Medical research ethics were approved, and all participating patients had given their written informed consent before enrolling in this study. Results: A total of 112 patients aged 63.2 ± 15.8 years participated in this study. The average cost of medication supplied by the inpatient pharmacy for the non-POM group was USD 21.60 ± 34.20 per patient, whereas, for the POM group, it was approximately USD 13.00 ± 18.30 per patient, with a mean difference of USD 8.60 ± 5.17 per patient (95% CI: −3.95, 27.47, p ≥ 0.05). The use of POM minimized 54.03% (USD 625.04) of the total cost spent by the hospital for the POM group within the period of the study. Conclusion: The pilot study showed that the supplied medication cost per patient was not significantly different between the POM and non-POM groups. Nevertheless, the utilization of POM during hospitalization is capable of reducing at least 50% of the total cost spent on inpatient medications by the hospital. The use of POM during hospitalization also helped in reducing the total time spent on the medication process per patient.
The Social Experiences of Single Gay Fathers in Israel: An Intersectional Perspective
Authors: Tsfati Maya , Segal-Engelchin Dorit
Published: September 2022

Through an intersectional lens, this study explores the experience of being a single gay father by choice in Israeli society, which continues to view the traditional heterosexual two-parent family as the ideal. Analysis of 15 in-depth, semi-structured interviews with single gay Israeli fathers suggests that their experiences are characterized by constant tension between their self-perceived social acceptance and sense of belonging on the one hand and their encounters with discrimination and bias on the other. The findings shed light on the ways in which the fathers’ complex experiences are shaped by the intersection between their identity as parents and their two marginalized identities: single male parents and gay fathers. These identities were found to be highly contextual and affected by macro-level factors. As the fathers cope with their environment, they develop a sense of belonging alongside a deep sense of otherness, suggesting that the sense of belonging and the sense of otherness are not binary opposites, but rather two social categories that are continually constructed through the intersection between different identities and their interaction with macro-level factors, making single gay parenthood a multidimensional experience.
Divergent Effects of Factors on Crash Severity under Autonomous and Conventional Driving Modes Using a Hierarchical Bayesian Approach
Authors: Ren Weixi , Yu Bo , Chen Yuren , Gao Kun
Published: September 2022

Influencing factors on crash severity involved with autonomous vehicles (AVs) have been paid increasing attention. However, there is a lack of comparative analyses of those factors between AVs and human-driven vehicles. To fill this research gap, the study aims to explore the divergent effects of factors on crash severity under autonomous and conventional (i.e., human-driven) driving modes. This study obtained 180 publicly available autonomous vehicle crash data, and 39 explanatory variables were extracted from three categories, including environment, roads, and vehicles. Then, a hierarchical Bayesian approach was applied to analyze the impacting factors on crash severity (i.e., injury or no injury) under both driving modes with considering unobserved heterogeneities. The results showed that some influencing factors affected both driving modes, but their degrees were different. For example, daily visitors’ flowrate had a greater impact on the crash severity under the conventional driving mode. More influencing factors only had significant impacts on one of the driving modes. For example, in the autonomous driving mode, mixed land use increased the severity of crashes, while daytime had the opposite effects. This study could contribute to specifying more appropriate policies to reduce the crash severity of both autonomous and human-driven vehicles especially in mixed traffic conditions.