bluuebunny/crossref_metadata_2025 huggingface dataset:
The opioid peptide biphalin modulates human corneal epithelial wound healing in vitro
Authors: Totuk Ozgun Melike Gedar , YILDIZ Erdost , MOLLICA Adriano , KABADAYI Kerem , SAHIN Afsun
Published: March 2020

Background: Analgesic drugs, including nonselective opioids and non-steroidal anti-inflammatory drugs, should be used with great precautions to relieve pain after physical damage of the corneal epithelium because of their unfavorable effects on the wound-healing process. Biphalin is a synthetic opioid peptide that has been demonstrated to possesses a strong analgesic effect on rodents. The purpose of this study is to investigate the effects of biphalin on human corneal epithelium wound healing.Methods: An immortalized human corneal epithelial cell (HCEC) culture was used to test the effects of biphalin on wound healing. The toxicity of biphalin in various concentrations was measured with the MTT assay. The effect of 1 µM and 10 µM biphalin were tested on wound closure in an in vitro scratch assay of HCECs and for cell migration and proliferation separately. Naloxone, a non-selective competitive antagonist of opioid receptors, was also used to inhibit the effects of biphalin in all experiments.Results: Biphalin did not cause any toxic effect on HCECs in concentrations lower than 100 µM at various incubation time points. Biphalin increased the wound closure process significantly at 1 µM concentration in an in vitro scratch assay of HCECs (p < 0.05). It also increased the migration of HCECs significantly (p < 0.01). There was no significant difference between biphalin and control groups of HCECs in the Ki67 proliferation assay.Conclusion: Biphalin, a synthetic opioid peptide, has a potential role as a novel topical analgesic agent that promotes corneal epithelial wound healing. This role should be evaluated in further in vivo and clinical studies.
TANA: efficient approach for predicting protein functions by transferring annotation via alignment networks
Authors: DJEDDI Warith Eddine , YAHIA Sadok BEN , NGUIFO Engelbert MEPHU
Published: March 2020

Background: One of the challenges of the post-genomic era is to provide accurate function annotations for orphan and unannotated protein sequences. With the recent availability of huge PPIs networks for many model species, the computational methods revealed a great requirement to elucidate protein function based on many strategies. In this respect, most computational approaches integrate diverse kinds of functional interactions to unveil protein functions by transferring annotations across different species by relying on similar sequence, structure 2D/3D, amino acid patterns or phylogenetic profiles. Results: In this work, we introduce a new approach, called TANA, for inferring protein functions. The main originality of the introduced approach stands on the function prediction for the unannotated protein by transferring annotation via a network alignment as well as from the direct interaction neighborhood within their PPI networks. Doing so, we are able to discover the functions of proteins that could not to be easily described by sequence homology. We assess the performance of our approach using the standard metrics established by the CAFA challenge and highlight a sharp significant improvement over other competitive methods, in particular for predicting molecular functions. Conclusions: This research is one of the first attempts that combine sequence and networks-multiple-alignment-based function prediction approaches. We have been able to assess the accuracy of the prediction using pairwise and multiple alignment of the PPI networks for the compared species. Therefore, we recommend using different strategies (i.e pairwise, multiple, with/without neighborhood networks) especially in situations where the functions of the protein are not known beforehand.
Prevalence of violence and associated factors among Youth in Northwest Ethiopia: Community-based Cross-sectional study.
Authors: Geremew Alehegn Bishaw , Gelagay Abebaw Addis , Bisetegn Telake Azale , Habitu Yohannes Ayanaw , Abebe Solomon Mekonen , Biru Eshetie Melese , Mekonen Temiro Azanaw , Ayele Yilikal Tiruneh , Yeshita Hedija Yenus
Published: March 2020

Background Violence as a known serious public health problem affects people in all stages of life, from childhood to the elderly. In society, one of the most visible forms of violence is young people violence, whereas they, adolescents and young adults, are the main victims of such violence. There was limited information on the burden of violence and factors among this age group. Therefore, this study aimed to determine the prevalence of violence and its associated factors among youth in Northwest, Ethiopia. Methods A community-based cross-sectional study design was conducted to estimate the magnitude of violence among youth in Northwest, Ethiopia. The calculated sample size was 1765. A structured and pretested interviewer administered questionnaire was used to collect the data. A multi-stage cluster sampling technique was employed to get the study participants. Data were entered using Epi Info version 7 and then exported to STATA 14 for data analysis. The bivariate logistic regression model was employed to identify associated factors. An adjusted odds ratio with a 95% confidence interval was used to determine factors associated with violence. Results From the total 1765 representatives of the youth population, 1597 (90.5%) youth participated in the study. Overall, the prevalence of violence among youth aged 15–24 years for the last 12 months was 21.5%. In the multivariate logistic regression model, factors significantly associated with violence were being married and divorced (AOR= 1.77, 95% CI: 1.13, 2.79) and (AOR=5.67, 95%CI: 2.93, 10.99), respectively, Living with mother’s only (AOR= 1.85, 95% CI: 1.28, 2.66) and father’s only (AOR=2.45, 95% CI: 1.30, 4.63), and substance use (AOR= 2.38, 95% CI: 1.56, 3.66). Conclusions The prevalence of violence among youth was high compared to other studies. Special emphasis should be given to reduce the burden of youth violence and preventing and controlling the identified factors by modifying the existing policies/strategies or developing new strategies and interventions program. Keywords: Violence, Youth, Physical violence, Sexual violence, Psychological violence
mRNA and microRNA profiles in the amygdala are relevant to Susceptible and Resilient mice induced by psychological stress
Authors: Sun Jinyan , Lu Yanjun , Yang Jiuyong , Song Zhenhua , Lu Wei , Wang Jin-Hui
Published: March 2020

Background Severe or prolonged stress increases the risk to develop psychopathologies. An individual's perception of stress exposure varies greatly, as do its consequences. Numerous individuals demonstrate resilience to psychological stress. The mRNA and microRNA profiles of stress susceptibility and resilience induced by psychological stress in the amygdala remain to be elucidated. Methods Psychological stress to an observer was given by witnessing the attacks of the aggressor on the similar. After five days of psychological stress, the degree of fear memory and anxiety of mice were measured by social interaction test and elevated plus-maze (EPM). mRNA and microRNA profiles were quantified by high-throughput sequencing in amygdala tissues harvested from Control, Susceptible and Resilient mice. Results In the amygdala from Susceptible mice versus Resilient mice, the upregulation of Peptide, Thyrotropin-releasing hormone, and ECM receptor, Glutamatergic synapse, Cytokine-cytokine receptor interaction, Long-term depression, PI3K-Akt, Oxytocin, GnRH, HIF-1, Estrogen, Calcium signaling pathways may be related to psychological stress-induced susceptibility, and the downregulation may be related to resilience. The downregulation of Adrenergic synapse, Adherens junction, Wnt, Sphingolipid, B cell receptor, cAMP, Rap1, Toll-like receptor signaling pathways may be related to psychological stress-induced susceptibility, and the upregulation may be related to resilience. Results by sequencing mRNA and microRNA profiles are consistent, in which some of them are validated by qRT-PCR and dual-luciferase reporter assay. Conclusion Susceptible and Resilient induced by psychological stresses are caused by the imbalanced regulation of different synapses and signaling pathways in the amygdala.
Perioperative pain control is important for prevention of venous thromboembolism after around the knee osteotomy
Authors: Kubota Mitsuaki , Kim Youngji , Sato Taisuke , Ohno Ryuichi , Ishijima Muneaki
Published: March 2020

Background: Venous thromboembolism (VTE), including deep venous thrombosis (DVT) and pulmonary embolism (PE) are well-known complications after TKA. However, there are few reports about VTEs after around the knee osteotomy (AKO). Purpose: To investigate the incidence of and risk factors for VTEs after AKO. Methods: A total 108 AKO cases were assessed in this study. The procedures included open-wedge high tibial osteotomy (OWHTO; n=96), hybrid closed-wedge high tibial osteotomy (CWHTO; n=7), distal femoral osteotomy (DFO; n=2), tibial condylar valgus osteotomy (TCVO; n=2), and double level osteotomy (DLO; n=1). Ultrasonography of the lower extremities was performed for all patients at 1 week after AKO. The incidence of VTEs was investigated, and a logistic regression analysis was performed to determine which patient demographics, pre-operative clinical results and surgical factors were associated with DVT or PE after surgery. Results: Of the 108 patients who underwent AKO, 29 (26.9%) developed postoperative DVT, but no patients developed PE. The logistic regression analysis identified abrasion chondroplasty (odds ratio [OR], 3.46) and pre-operative pain visual analog scale (VAS; OR, 1.05) as independent predictors of DVT. Conclusion: The incidence of DVT after AKO is relatively high and preoperative and postoperative pain may induce DVT. Perioperative pain control is important for preventing VTEs.
The new nutrition label is a combination of the physical activity, traffic light and warning labels: a qualitative study
Authors: Seyedhamzeh Shirin , Nedjat Saharnaz , Shakibazadeh Elham , Hosseini Hedayat , Doustmohammadian Azam , Dorosty Ahmad
Published: March 2020

Background: Unhealthy diets and inadequate physical activity are important factors in the increasing trend of obesity. In order to mitigate this harmful trend, an effective policy must be developed to target both unhealthy diets and insufficient physical activity. The implementation of physical activity recommendations in nutrition labeling may influence the consumers’ food choices. In this qualitative study, we designed a new type of physical activity calorie equivalent (PACE) label by considering the viewpoints of household representatives and experts.Method: Ten focus group discussions (FGDs) were conducted with 63 mothers from different regions of Tehran based on the participants’ socioeconomic status. Furthermore, ten interviews were conducted with food quality control (FQC) experts to examine the new PACE label. The preliminary designs were based on the mothers’ viewpoints. We then presented these new labels to the nutritionists and food industry experts in two separate FGDs. After taking their opinions into consideration, we applied certain changes to the labels and emailed them to the mothers. Consequently, the final versions of the new labels were designed. Conventional content analysis was applied with the MAXQDA10 software.Result: Two main themes were defined: (1) the stakeholders’ perspectives on PACE labeling (2) the new PACE label’s characteristics including the appearance and the nutritional information on the new label.Conclusion: The new label is a combination of the physical activity, traffic light and warning labels.
Effect of brain lesions on voluntary cough in patients with supratentorial stroke: an observational study
Authors: Lee Kyoung Bo , Lim Seong Hoon , Park Geun-Young , Im Sun
Published: March 2020

Background: Patients with stroke are known to manifest decreased cough force, which may increase the risk of aspiration. Specific brain lesions have been linked to impaired reflexive cough. However, only a few studies investigated whether specific stroke lesions are associated with impaired voluntary cough. The present study analyzed the effects of stroke lesion on voluntary cough using voxel-based lesion-symptom mapping (VLSM).Methods : In this retrospective cross-sectional study, the peak cough flow was measured in patients who complained of weak cough (n = 39) after supratentorial lesions. Brain lesions were analyzed via magnetic resonance imaging (MRI) at the onset of stroke. These lesions were analyzed using VLSM.Results : The VLSM method with non-parametric mapping revealed that lesions in the sub-gyral frontal lobe, superior longitudinal and posterior corona radiata were related to weak cough flow. Also, lesions in the inferior parietal and temporal lobes, and both superior and mid-temporal gyrus were also associated with weak peak cough flow during voluntary coughing.Conclusions : The present study identified several brain lesions underlying impaired voluntary cough. The results may be useful in predicting those at risk of poor coughing function and may facilitate the prognosis of patients at increased risk of respiratory complications after stroke.
Dipyridamole-thallium scanning doesn't reduce 90-day cardiac complications and mortality in femoral neck fracture patients undergoing hemiarthroplasty: a propensity-score-matched analysis in an Asian population
Authors: Liao Chin-Yi , Tan Timothy L , Lu Yu-Der , Wu Cheng-Ta , Kuo Feng-Chih , Lee Mel S
Published: March 2020

Background: This study aimed to evaluate the effect of dipyridamole-thallium scanning on 90-day cardiac complications and mortality in patients with femoral neck fracture treated with hemiarthroplasty.Methods: Between 2008 and 2015, 844 consecutive patients undergoing cemented or cementless hemiarthroplasty were identified from the database of a single level-one medical center. One-hundred and thirteen patients (13.4%) underwent the dipyridamole-thallium scan test prior to surgery, and 731 patients (86.6%) did not. Patient characteristics, comorbidities, surgical variables, and duration to surgery were recorded. Multivariate logistic regression was performed, and a propensity-score-matched cohort was identified in order to determine whether dipyridamole-thallium scanning was associated with 90-day cardiac complications and mortality.Results: The incidence of 90-day cardiac complications was 19.47% in the thallium scan group and 11.58% in the non-thallium scan group (p = 0.343) among 452 patients after propensity score-matching. The 90-day mortality rate was similar in the two groups (0.88% vs. 1.18%, p = 0.795). In the propensity score-matched patients, utilization of the dipyridamole-thallium scan was irrelevant to cardiac complications (matched cohort, adjusted odds ratio [OR] = 1.32; 95% confidence interval [CI] 0.75 to 2.33) or mortality (adjusted OR = 0.75; 95% CI 0.08 to 6.71) within 90 days. Risk factors for cardiac complications were an American Society of Anesthesiologists grade 3 (OR 3.19, 95% CI 1.44 to 7.08) and pre-existing cardiac comorbidities (OR 5.56, 95% CI 3.35 to 9.25). Discussion: Preoperative dipyridamole-thallium scanning was not associated with reductions in 90-day cardiac complications and mortality in patients with femoral neck fracture undergoing hemiarthroplasty. The necessity for dipyridamole-thallium scanning should be re-evaluated in elderly patients with femoral neck fractures, given that this increases the duration to surgery.
Comparison between subcutaneous pocket and cryopreservation method for storing autologous bone flaps in developing bone flap infection after cranioplasty, a prospective observational multi-center study of 100 cases
Authors: Shafiei Mehdi , Sourani Arman , Korki Amir Arsalan , Mahram Saeed
Published: March 2020

Background Following a craniotomy, harvested bone flap, if available, is stored for future cranioplasty. There are two different methods for bone banking: subcutaneous pocket in abdominal wall and cryopreservation in a refrigerator. This study is designed to evaluate risk of developing infection in each group, retrospectively. Methods In this prospective observational multi-center study, one hundred (100) patients who underwent cranioplasty with autologous bone flap were divided into two groups of 50 patients. rate of clinical post-operative infection and possible associated risk factors were analyzed with statistical measures. The data check lists parameters were indication of DC, CP vs SP, interval between craniotomy and cranioplasty, post-operative hospitalization duration, new morbidities and possible mortalities. The data were analyzed by an expert bio-statistician with proper bio-statistical methods (p<0.05) Results Four (4) patient in cryopreservation group (50 patients) had post-operative bone flap infection (8%).in subcutaneous pocket method no post-operative infection after cranioplasty was noticed, which are not statistically significant (p=0.054). over-all post-operative infection rate was 4%. Conclusion There were no significant differences in post-operative infection rate between cryopreservation versus subcutaneous pocket method in storing bone flaps. Older age maybe associated with infection development after cranioplasty.
A prognostic nomogram based on competing endogenous RNA network for clear-cell renal cell carcinoma
Authors: Peng Yun , Wu Shangrong , Xu Zihan , Hou Dingkun , Li Nan , Zhang Zheyu , Wang Lili , Zhang Yangyi , Wang Haitao
Published: March 2020

Backgroud Clear-cell renal cell carcinoma (ccRCC) is stubborn to traditional chemotherapy and radiation treatment, which makes its clinical management a major challenge. Recently, we have made efforts to understand the etiology of ccRCC. Increasing evidence revealed that the competing endogenous RNA (ceRNA) were involved in the development of various tumor. However, it’s scant for studying on ccRCC, and a comprehensive analysis of prognostic model based on lncRNA-miRNA-mRNA ceRNA regulatory network of ccRCC with large-scale sample size and RNA‐sequencing expression data is still limited. Methods RNA‐sequencing expression data were taken out from GTEx database and TCGA database, A total of 354 samples with ccRCC and 157 normal controlled samples were included in our study. The ccRCC-specific genes were obtained from WGCNA and differential expression analysis. Following, the communication between mRNAs and lncRNAs and target miRNAs were predicted by MiRcode, starBase, miRTarBase, and TargetScan. A gene signature of eight genes was constructed by univariate Cox regression, lasso methods and multivariate Cox regression analysis. Results A total of 2191 mRNAs and 1377 lncRNAs was identified, and a dys-regulated ceRNA network for ccRCC was established using 7 mRNAs, 363 lncRNAs, and 3 miRNAs. Further, a gene signature in cluding 8 genes based on this ceRNA was constructed, meanwhile, a nomogram predicting 1-, 3-, 5-year survival probability containing both clinical characteristics and ccRCC-specific gene signatures was developed. Conclusion It could contribute to a better understanding of ccRCC tumorigenesis mechanism and guide clinicians to make a more accurate treatment decision.
Vertebrae rotation made little difference on the position of abdomial aorta relative to spine in adult degenerative scoliosis
Authors: Liang Yan , Xu Shuai , Zhu Zhenqi , Liu Haiying , Meng Fanqi
Published: March 2020

Background: Few study has been published for quantitative relation of the vertebrae rotation on the position of the aorta relative to spine in adult degenerative scoliosis (ADS). Thus, this study was to explore the effect of the vertebrae rotation on the position of the abdomial aorta relative to spine in patients with ADS and identify the risk factor of vertebrae rotation.Methods: A retrospective analysis was performed for 71 patients with ADS divided into left scoliosis (LS group, 40cases) and right scoliosis (RS group, 31cases). The two group were well-matched in demographics. Apical vertebrae, Cobb angle (°), coronal displacement and thoracolumbar kyphosis (TLK) were measured on X-ray. Aorta-vertebrae angle (α), aorta-vertebrae distance (d) and rotation angle (γ) for each level of T12-L4 on MRI were obtained within a Cartesian coordinate system.Result: There was no significant difference in apical vertebrae distribution between the LS and RS group, so were Cobb angle and coronal horizontal displacement distance (P˃0.05). There was no significance on mean γ of of apical vertebrae among different levels in LS group and RS group (P ˃0.05). Pearson correlation analysis showed γ was a positively correlated to Cobb angle and coronal horizontal displacement distance (P<0.001) in both groups. There was no significant correlation between the γ and α, d, whichever the group (P˃0.05). Coronal movement was the independent risk factor of γ in LS group with the equation of γ = -4.502+0.542×Coronal movement while Cobb angle and coronal horizontal movement were risk factors in RS group with γ = 2.953-0.512×Cobb angle -0.406×Coronal movement.Conclusion: Vertebral rotation could be predicted by Cobb angle or coronal movement by X-ray in ADS and aorta maintained a relatively normal position in patients with ADS.
Cloning and functional analysis of JnCYCD3;1 in Jatropha nigroviensrugosus
Authors: Feng Xiao , Yang Zhao , Xiu-Rong Wang
Published: March 2020

Background: As a new variety of Jatropha, the female to male flower ratio and yield of Jatropha nigroviensrugosus are higher than the common J. curcas. Using pre-transcriptome data, the full-length gene sequence, subcellular function localization, and verification of the transgenic function were obtained in order to understand the specific functions of differentially expressed genes (DEGs). Results: Results revealed that the open reading frame (ORF) of J. nigroviensrugosus CYCD3;1 (JnCYCD3;1) was 414 bp long, encoding 137 amino acids (aa). Compared to J. curcas, the presence of intron retention led to early termination of the coding frame. JnCYCD3;1 had the highest expression in new leaves, which was 68.42 times root expression, followed by inflorescence buds. The JnMYC2 ORF was 2025 bp, encoding 674 aa. JnMYC2 had the highest expression levels in inflorescence buds. JnCYCD3;1 functioned in the nucleus, while JnMYC2 was distributed in both the nucleus and cytoplasm and may possess transmembrane membrane behavior. Bimolecular fluorescence complementation (BIFC) experiments indicated that JnMYC2 interacted with JnCYCD3;1. JnCYCD3;1 transgenic tobacco considerably advanced the reproductive cycle and may promote flower formation and transformation. Conclusions:The related experiments obtained new CYCD3;1 transcript, verified that CYCD3;1 is related to flower bud differentiation, proved the interaction between hormones-related genes. The study provides a new research direction for gene function of CYCD3;1.
The molecular basis of loss of smell in 2019-nCoV infected individuals
Authors: Gupta Krishan , Mohanty Sanjay Kumar , Kalra Siddhant , Mittal Aayushi , Mishra Tripti , Ahuja Jatin , Sengupta Debarka , Ahuja Gaurav
Published: March 2020

Among the prominent clinical symptoms such as fatigue, shortness of breath, fever, and cough, 2019-nCoV infected individuals often experience hyposmia/anosmia (decrease or loss of sense of smell). Angiotensin I Converting Enzyme 2 (ACE2), a key host receptor has now been established as an important moiety for the entry of 2019-nCoV into the host cells. A multitude of studies estimated the expression of ACE2 in multiple organs including heart, kidney, intestines, lungs, buccal cavity, etc. The ongoing medical examinations and the autopsy reports of the diseased individuals strongly corroborate these organ/tissue-level molecular insights. Olfactory mucosa harbors multiple functionally distinct cell types. Zeroing in on the cell lineages that underpin infection associated loss of olfaction may provide new leads for diagnostics/clinical management of 2019-nCoV infected individuals. Our pointed bioinformatic analysis of single-cell expression profiles underscored selective expression of ACE2 in a subset of horizontal basal cells (HBCs) and sustentacular cells (SUSs) of the olfactory mucosa in humans. Inspection of the ACE2 levels in the olfactory mucosa of 4 additional mammalian species revealed comparable expression patterns, indicating the risk of olfactory dysfunction in these species. In summary, our findings pinpoint the molecular rationale of loss of smell in 2019-nCoV infected patients.
ASCUS Negative Conversion in Pelvic Floor Reconstruction of POP Patients with Human Papillomavirus-Negative ASCUS Cytology
Authors: Wei Ai-Min , Fan Yun , Shen Yu-Fei , Zhang Lei , Tan Xiao-Mei
Published: March 2020

Objective To inquire into the influence of the pelvic floor reconstruction surgery of pelvic organ prolapse on Papillomavirus-Negative atypical squamous cells of undetermined significance cytology. Methods This investigation was studied retrospectively about female patients of POP 3~4 stage with high-risk human papillomavirus DNA-negative ASCUS between January, 2008 and September, 2018.All patients received one of two treatment regimens, pelvic floor reconstruction or local hydrocortisone cream(0.1% Hydrocortisone Butyrate cream, 1g nightly for no less than 12 weeks). After 6 months follow-up, we collected data to compare the positive rate of ASCUS with high-risk human papillomavirus DNA-negative between the two groups after intervention. Results The negative conversion rate of ASCUS in the operative patients was higher than in patients using local hydrocortisone cream (33/47 [70.2%] vs 19/44 [43.2%], P = 0.009). Conclusions The decline of non–human papillomavirus ASCUS cytology in postoperative patients might be related to pelvic floor reconstruction.
CRISPR/Cas9-mediated microRNA-21 Cleavage Sensitizes the Treatment Response to Dasatinib in Chronic Myeloid Leukemia Cell Line K562
Authors: Zhang Yun , Wang Lingyan , Li Jiazheng , Jiang Peifang , Lin Minhui , Hu Jianda , Chen Buyuan
Published: March 2020

Background: Tyrosine kinase inhibitors (TKIs) targeting BCR-ABL1 kinase are effective in treating chronic myeloid leukemia (CML), but TKI resistance occurs in a significant number of patients, and the underlying molecular mechanisms of this resistance remain largely unknown. As an oncomiR, microRNA-21(miR-21) functions directly in drug resistance, but its relationship with TKI resistance in CML is rarely reported. As a novel and effective gene editing tool, clustered regularly interspaced short palindromic repeats–CRISPR-associated protein 9(CRISPR/Cas9) has certain advantages in completely knocking out target genes at the gene level. Methods: We successfully constructed lentiviruses LV-VMP1-sgRNA (045001) and human chronic myeloid leukemia K562 cells were transducted with them. Single-cell-derived clones were screened for miR-21 deletion by genomic DNA PCR and Sanger sequence. RQ-PCR assays was used to confirm the knockout of miR-21. 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide(MTT) and colony formation assays were applied to assess the cell growth inhibition. Imatinib and dasatinib sensitivity was determined by MTT assay and Annexin-V APC/7-AAD double staining flow cytometry. Western blot assay was performed to measure the levels of Phosphatase and tensin homolog(PTEN), Phosphatidylinositol 3-kinase(PI3K), Serine/threonine Kinase(AKT), p-AKT, BCR-ABL(P210), p-BCR-ABL (p-P210) . Result: miR-21 knockout inhibited proliferation of K562 cells, promoted their apoptosis and increased their sensitivity to dasatinib. Further mechanism studies suggest that this is achieved by inhibiting the PI3K/AKT signaling pathway and destroying BCR-ABL. Conclusions: Our study reveals the efficacy of CRISPR/Cas9 gene editing to miR-21 in K562 and indicates miR-21 as a potential target in sensitizing dasatinib treatment for CML patients with pool response to the TKI targeting therapy.
Effects of StAAP1 and StAAP8 in Potato ATFs Transcription Factor Family on Plant Development
Authors: Zhang Chao , Kong Nana , Si JiaXin , Wang Dongdong , Chen Qin
Published: March 2020

Background: Amino acid transporters mediated the transport of various amino acids in plants, which were essential for plant growth and development. ATFs (the amino acid transporter family) was one of amino acid transporters in plants and AAPs (amino acid permease) subfamily belongs to the ATFs family.Results: In this research, eight AAP genes were identified in the potato and divided into two subgroups. The first subgroup is the StAAP gene containing six microporous transport channels (Pore-lining), and the second subgroup are the StAAP gene containing seven microporous transport channels. The subcellular location and overexpression vectors were built by StAAP1 and StAAP8. The results indicated these two genes were localized on the cell membrane. Observing the phenotype of the transgenic plants, StAAP1 and StAAP8 promoted the growth of leaves, StAAP1 changed the shape of tubers and enhanced their weight and StAAP8 was no significant influence in tubers but decreased the weight a little.Conclusions: In this study, the expression pattern showed StAAP8 might regulate the transport of amino acids in potato roots and affect the osmotic potential of potato root cells. Thereby, the tolerance of plants to abiotic stress and StAAP1 and StAAP8 promote the amino acid transport into leaves and StAAP1 had effected on tuber development and StAAP8 might resist the weight of tubers.
The impact of COVID-19 on ischemic stroke: A case report
Authors: Zhai Pan , Ding Yanbing , Li Yiming
Published: March 2020

BackgroundThe outbreak of a novel coronavirus since December 2019 in Wuhan, became an emergency of major international concern. As of March 5, 2020, the SARS-CoV-2 epidemic has caused 80,565 confirmed infections with 3,015 fatal cases in China. The SARS-CoV-2 outbreak is a major challenge for clinicians. In our clinic, we found a rare case that a COVID-19 patient combined with ischemic stroke.Case PresentationA 79-year-old man was admitted to the Hubei Provincial Hospital of Chinese Traditional Medicine due to right limb weakness for 1 day and slight cough for 1 week. At presentation, his body temperature was 37.3°C (99.0°F) with some moist rales. Neurological examination showed right limb weakness, and the limb muscle strength was grade 4. The left leg and arms were unaffected. In addition, runs of speech were not fluent enough with tongue deviation. Laboratory studies showed lymphopenia and eosinophilic granulocytopenia. Chest CT revealed bilateral pulmonary parenchymal ground-glass and consolidative pulmonary opacities, with a peripheral lung distribution. Real-time polymerase chain reaction (RT-PCR) from throat swab sample was positive for SARS-CoV-2 nucleic acid. This patient was treated with antiviral drugs and anti-inflammatory drugs with supportive care until his discharge. Clopidogrel (75 mg) and atorvastatin (20 mg) were administered orally to treat acute ischemic stroke. After twelve days of treatment, he can walk normally and communicate with near fluent language.ConclusionWe report an even more unusual case, a patient who was hospitalized for right limb weakness and was later diagnosed with COVID-19. Here, SARS-CoV-2 infection caused hypoxemia and excessive secretion of inflammatory cytokines, which contribute to the occurrence and development of ischemic stroke. Once COVID-19 patients show acute ischemic stroke, neurologists should cooperate with infectious disease doctors to help patients.
Outbreak Measures Taken by Medical Schools During the Coronavirus Pandemic in London, United Kingdom: A Qualitative Study
Authors: Ting Andrew Ser Jien , Ho Carmen Lok Tung
Published: March 2020

Background: Coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first reported in Wuhan, China in December 2019. On 11 March 2020, the World Health Organization (WHO) declared COVID-19 to be a pandemic. As of mid-March 2020, more than a total of 150,000 cases have been reported in 122 countries, including 1,543 in the United Kingdom. Within London, there are five universities with medical schools each faced with difficult decisions on how to respond to this unprecedented situation, having to balance the education of future doctors who will soon be joining the front lines and their safety. In this paper, the responses and timeliness of medical schools are collated and compared. This will help guide medical schools’ responses in the future.Methods: Information was gathered from the official university websites and social media platforms. Thematic analysis was performed to obtain overarching categories of responses by the medical schools.Results: All five medical schools displayed similar responses to COVID-19, following guidance provided by Public Health England (PHE), Foreign & Commonwealth Office (FCO) and Medical Schools Council (MSC). Eight broad themes of responses were identified to have been undertaken by most London medical schools. Responses such as suspending clinical placements, keeping university facilities open and not banning on-campus events were universally adopted by all five medical schools. Other responses such as specific exam rearrangements and elective travel advice were more heterogeneous amongst the medical schools.Conclusion: Medical schools must take extraordinary measures in response to a pandemic. The experience gained from the COVID-19 pandemic will help future administrations be more confident in providing a more rapid response to similar health crises.
Stability analysis of floating raft system under multiexcitation condition
Authors: Li Xin , Zhang Jinqiu
Published: March 2020

A floating raft system is subjected to multiple excitation sources with multiple frequencies for each excitation source. Considering the two characteristics of excitation source, the stability of floating raft system was analyzed. A vibration equation for the floating raft system under multiexcitation condition was established. A multiscale method was then used to solve the equation. The amplitude–frequency response equation and unstable region of solution are discussed. The results show that the vibration of raft frame fits the pattern of soft-spring vibration. This indicates that the excitation of main raft unit with a rigid connection compromises the stability of the system, whereas the excitation of unit with elastic connection increases stability.
Seismic dynamic responses of earth-rock dam considering wave and seepage
Authors: Jing Wei , Wang Jingxuan
Published: March 2020

Earth-rock dam plays an important role in water conservancy project, but there were many damage cases caused by earthquakes, so it is necessary to investigate dynamic responses of earth-rock dams under combined action of earthquake and wave. Considering material nonlinearity, seepage, wave action, fluid-solid interaction, liquid sloshing, a numerical calculation model is established, and Arbitrary Lagrange Eulerian method is used to solve the nonlinear dynamic coupling problem. Dynamic responses under wave, earthquake and wave+earthquake are studied comparatively, and influences of near and far field earthquakes and PGA on dynamic responses are investigated. Results show that values and distributions of dynamic responses under wave and earthquake are quite different, and dynamic responses were further increased considering combination of earthquake and wave. Dynamic responses under far field long period earthquake is significantly larger than that under near field earthquake. With the increase of PGA, increase trends of horizontal displacement, vertical displacement and plastic strain are increased, while increase trend of maximum shear stress is slowed down. It can be obtained that when earthquake intensity is large, earth-rock dam will be easily damaged due to excessive deformation.
Entre el miedo y la distinción. El estado actual del fraccionamiento cerrado en las ciudades fronterizas de Tijuana, Nogales y Ciudad Juárez
Authors: Enríquez Acosta Jesús Ángel
Published: March 2020

En las ciudades de Tijuana, Nogales y Ciudad Juárez, ubicadas en la frontera noroeste entre México y Estados Unidos, uno de los procesos de urbanización que se ha extendido en los últimos años es la modalidad del fraccionamiento cerrado. Estas áreas residenciales se caracterizan por ser espacios físicos protegidos y separados de los disturbios de la ciudad por bardas y rejas, y en su mayoría presentan un acceso restringido por medio de casetas, plumas y seguridad proporcionada por guardias privados. El concepto de fraccionamiento cerrado tiende a expandirse en el paisaje urbano de las ciudades fronterizas sin más restricciones que las que el mercado inmobiliario se impone a sí mismo, no obstante, es importante analizar su impacto en la morfología de las ciudades, la sustentabilidad urbana de estos desarrollos y la segregación social que propician.
Cooperación y desarrollo territorial en el espacio fronterizo del norte de Marruecos
Authors: López Lara Enrique
Published: March 2020

El norte de Marruecos, espacio regional fronterizo con España y la Unión Europea, es objeto de un intenso proceso de fomento de políticas y de programas de cooperación de diversas instituciones (ONU, Unión Europea, agencias de cooperación, entre otras) que poseen intereses en la zona, como son: fortalecimiento de un anillo de seguridad en un espacio estratégico, crecimiento de la renta por la existencia de un elevado escalón entre vecinos, creación de una nueva política de vecindad basada en la semiintegración, reducción de la emigración, etcétera. Cuestión que coincide con una decidida política del gobierno marroquí de relanzar socioeconómicamente este espacio: proceso de regionalización y dotación de instituciones (Agencia para la Promoción del Desarrollo en las Regiones del Norte), diseño de una estrategia de crecimiento basada en el desarrollo humano, y otras más. En este documento se analizan las razones de este proceso, la realidad geográfica y la caracterización socioeconómica del espacio fronterizo y se diagnostican debilidades y oportunidades para la articulación de las políticas de cooperación de los diferentes actores que buscan un desarrollo territorial de este espacio fronterizo hoy en crisis.
Plan of tests with addition. Efficient estimate of dependability indicators
Authors: Mikhailov V. S.
Published: March 2020

The Aim of the paper consists in improving the efficiency of dependability indicator estimation for the plan of tests with addition, i.e. probability of no-failure and mean time to failure. Due to economic considerations, determinative dependability tests of highly dependable and costly products involve minimal numbers of products, expecting failure-free testing or testing with one failure, thus minimizing the number of tested products. The latter case is most interesting. By selecting specific values of the acceptance number Q and number of tested products, the tester performs a preliminary estimation of the dependability indicator, while selecting Q = 1 the tester minimizes the risks caused by an unlikely random failure. However, as the value Q grows, the number of tested products does so as well, which makes the testing costly. Therefore, the reduction of the number of products tested for dependability is the firstpriory problem and, in this context, economic planning of testing with addition is becoming increasingly important. We will consider binomial tests (original sample) with addition of one product (oversampling) to testing in case of failure of any of the initially submitted products. Testing ends when all submitted products have been tested with any outcome (original sampling and oversampling). Hereinafter it is understood that the testing time is identical for all products. Testing with the acceptance number of failures greater than zero (Q > 0) conducted with addition allows reducing the number of tested products through successful testing of the original sample.

Methods. Efficient estimation is based on the integral approach formulated in many papers. The integral approach is based on the formulation of the rule of efficient estimate selection specified on the vertical sum of absolute (or relative) biases of estimates selected out of a certain set based on the distribution law parameter, where n is the number of products initially submitted to testing. The criterion of selection of an efficient estimate of the probability of failure (or PNF) at a set of estimates is based on the total square of absolute (or relative) biases of the mathematical expectation of estimates from probability of failure p for all possible values of p, n.

Conclusions. The paper examines the probability of no-failure estimates for the plan of tests with addition. For the case of n > 3, the estimates and composite estimate are more efficient in comparison with estimate . The composite estimate of the probability of no-failure should be used in failure-free tests. For the case of n > 3, testing with the acceptance number of failures greater than zero (Q > 0) conducted with addition allows reducing the number of tested products through successful testing of the original sample. The composite estimate of the mean time to failure is bias-efficient among the proposed mean time to failure estimates. The obtained composite estimates and are of practical significance in the context of failure-free testing with addition.
On the consideration of progressive failure at the stage of design
Authors: Dolganov A. I.
Published: March 2020

The stress that affects structures and their mechanical and geometrical parameters are random values. For that reason, the dependability of a construction facility (technical system) is generally evaluated in terms of the probability of no-failure over the estimated period of operation. The paper shows the feasibility of dependability analysis of building systems in the course of their design using logical and probabilistic methods, presents algorithms for regulating their dependability. It examines the feasibility of assuring the dependability of a construction project using the example of a double-span whole hinged beam. The paper also establishes the requirement of accounting for all possible destruction models of a building system. The dependability of a double-span whole hinged beam is estimated based on the probability of non-occurrence of all possible destruction models or one of a set of possible kinematic mechanisms. A kinematic mechanism forms a chain of plastic hinges or a chain of progressive failures of effective sections. In other words, the task of preventing progressive collapse comes down to ensuring the required dependability of both the building as a whole, and its individual members (effective sections) by adjusting qualitative and quantitative indicators of the dependability structure. The dependability of a member is understood as its ability to maintain internal force within the effective section at least as high as the external force. It is shown that correct design solutions, rational choice of materials and load non-exceedance probabilities enables specified dependability of a building system. In some cases that allows saving materials, in others enables lower probabilities of failure. Constructing the dependability structure of a technical system enables a quantitative estimation of the most hazardous design models of destruction, rational management of the choice of safety factors of load bearing members, redistribution of such safety factors, thus preventing progressive collapse. The introduced differential characteristics of the members’ “weight”, “significance”, “contribution” and “specific contribution” allows demonstrating the distribution of the roles of each member within the specified structure in terms of specific problems, including accounting for the possibility of progressive collapse. The study has shown that the removal of undependable vertical load bearing structures does not solve the problem of dependability of a construction project, including protection against progressive collapse. It has been established that the design of structures, including in terms of considerations of progressive failure, must involve constructing a system dependability structure using kinematic analysis, identifying the most important and significant members of such structure and – using special adjustment techniques – obtaining the required structure dependability. That will enable significant resource saving and reduction of costs associated with the development of construction operations.
PREFACE
Authors: Lima Clóvis Ricardo Montenegro de
Published: March 2020

Preface
EXPEDIENTE
Authors: Lima Clóvis Ricardo Montenegro de
Published: March 2020

Expediente
ASPECTOS DE SEGURANÇA DE DADOS NA INOVAÇÃO ABERTA: revisão da literatura
Authors: Campos Junior Pedro Silvino , Mendes Junior Ricardo
Published: March 2020

Atualmente as empresas tem buscado, como forma de diferenciação dentro do mercado, processos inovadores em especial a inovação aberta. Há entretanto uma preocupação genuína em entender de que forma o processo de inovação pode impactar a segurança de informação e por consequência o negócio. Este estudo tem como objetivo analisar a produção científica em inovação aberta e segurança da informação no período de 2008 a 2018. Pretende-se verificar oportunidades de avanço do tema. Foram encontrados 85 artigos relacionados aos dois temas.  Os resultados mostram que apesar do crescimento recente em torno do tema, há muito pouco material em língua inglesa e os disponíveis em língua portuguesa são quase inexistentes. A análise constatou que a maioria dos artigos são referentes a pesquisa exploratória e que englobam mais especificamente um dos temas pesquisados: inovação ou segurança da informação, e que a temática de interseção desses dois temas é extremamente rara, o que pode indicar uma oportunidade de avanço em pesquisas acadêmicas.Palavras-chave: Inovação. Inovação aberta. Propriedade intelectual. Segurança da informação.
Benefícios da dança de salão na imagem corporal das mulheres histerectomizadas
Authors: Guida Letícia Sampaio , Andrade Maria Clara de Mello
Published: March 2020

O presente trabalho tem como objetivo investigar os benefícios da dança de salão em mulheres que realizaram a cirurgia de histerectomia. Esse procedimento pode ser muito desgastante para a maioria das mulheres, uma vez que pode trazer efeitos subjetivos relacionados à sexualidade. A dança de salão pode ser benéfica para quase todas as pessoas, e, principalmente para a população supracitada, auxiliando na reconstrução da imagem corporal, refletindo em sua qualidade de vida. Por esse motivo, nas intervenções psicológicas, o profissional de Psicologia deve indicar a suas pacientes histerectomizadas esse tipo de atividade.  O estudo se justifica pelo grande número de mulheres que passam por este tipo de cirurgia e enfrentam toda uma gama de problemas daí advindos, no Brasil e no mundo. O trabalho foi desenvolvido por meio de revisão de literatura numa abordagem qualitativa, observando que a dança de salão contribui para que a mulher tenha uma melhor qualidade de vida, desenvolvendo uma autoimagem mais favorável. Dessa maneira, o profissional de Psicologia, nas intervenções psicológicas, com mulheres histerectomizadas, deve incentivar suas pacientes para as práticas de dança de salão.