bluuebunny/crossref_metadata_2025 huggingface dataset:
Digital Transformation in University Architecture: Optimizing Construction Processes and User Experience through CAMPUS 2.0 at Pontificia Universidad Javeriana
Authors: Carrasco-Beltrán Daniela , Serrano-Sierra Alejandro , Cuervo Roberto , Valbuena-Bermúdez Carolina , Pavlich-Mariscal Jaime A. , Granados-León César
Published: September 2024

The integration of digital technologies in managing technical and design information is transforming architecture, engineering, and construction (AEC) processes within educational institutions. Despite this, construction education lacks practical, interactive learning tools, and there is insufficient collaboration between academia and the construction industry. To address these challenges, the CAMPUS 2.0 project at Pontificia Universidad Javeriana developed a web-based platform that integrates building information modeling (BIM) and gamification elements. This platform improves project coordination, facilitates interdisciplinary learning, and enhances the management of technical and design information for campus buildings. CAMPUS 2.0 also promotes collaboration and active user engagement, filling a critical gap in the practical tools in construction education. This study assesses the usability of CAMPUS 2.0 among 235 students, teachers, and staff members, demonstrating a positive impact on the university community. The findings provide insights into how digital tools can improve project management, interdisciplinary collaboration, and knowledge sharing within educational settings, offering broader implications for other institutions.
Behavior of Cellulosic Fiber Board Wood-Frame Shear Walls with and without Openings under Cyclical Loading
Authors: Musselman Eric S. , Dinehart David W. , FitzPatrick Thomas , Zabel Richard
Published: September 2024

Cellulosic fiber board (CFB) is a lightweight form of sheathing that contains 94% post-consumer recycled materials. The viability of CFB sheathed shear walls as an alternative to Oriented Strand Board (OSB) walls is the focus of this study. A total of 23 walls and 10 connection samples were tested under cyclical loading to determine their overall behavior and capacity. The walls consisted of 2.44 m high by 3.66 m long CFB and OSB sheathed walls with and without openings. The design capacity of each wall was calculated and compared to the experimental results. For the walls with openings, the effects of blocking and strapping on their behavior were also evaluated. It was found that CFB is a viable alternative to OSB; however, some adjustments to the current design values and processes are required as the current procedure results in safety factors that are significantly lower than those for OSB walls.
Analysis of Equivalence between Loading Rate and Stress Level of Fatigue Characteristics of Asphalt Mixture
Authors: Tao Liang , Liu Zhengnan , Ding Xinglan
Published: September 2024

The accurate characterization of fatigue life affects the durability and reliability of asphalt pavement for the asphalt mixtures. This paper proposed a new fatigue test method and analysis method which, in addition to increasing the accuracy of fatigue characterization, reduces the time and cost consumed in laboratory tests of asphalt mixtures. According to the loading speed corresponding to different loading frequency and stress levels, the corresponding strength value is selected to determine the stress ratio in the fatigue equation. Therefore, the stress ratio can truly reflect the fatigue loading stress conditions and correspond to the fatigue loading speed one by one, avoiding the unscientific fatigue resistance design problem caused by the traditional S-N fatigue equation stress ratio, which takes a single strength value at a constant loading speed as the standard. Since the stress ratio takes into account the influence of loading speed under different loading frequencies and stress levels, a unified representation model of the fatigue equation of the asphalt mixture under different loading frequencies can be established; that is, the fatigue performance of the asphalt mixture under different loading frequencies can be normalized. The results showed that the loading rate in the strength test differed from the loading rate in the traditional fatigue test, which means that the fatigue curve could not be extended to point (1,1) when the fatigue equation was extended to a fatigue life Nf = 1. The fatigue test related to the velocity-dependent stress ratio in multiple experimental works, and data discreteness had a remarkable influence on fatigue characterization. The fatigue curve obtained by the fatigue equation under a constant loading rate was consistent with the fatigue curve under different loading rates. This method can reduce the duration of strength tests and the impact of the strength results of discreteness on fatigue characterization. The fatigue characterization model under constant loading rates considered the viscoelasticity of asphalt mixtures and guaranteed the correspondence of test conditions among stress level t, standard strength St, and fatigue life Nf. The stress levels and loading rates of the asphalt mixtures were confirmed to be equivalent. Furthermore, this paper established the relationship between strength and fatigue and obtained the fatigue life curve of the strength values. The fatigue performance of the asphalt mixtures is evaluated comprehensively.
Bridge Damage Identification Based on Encoded Images and Convolutional Neural Network
Authors: Wang Xiaoguang , Li Wanhua , Ma Ming , Yang Fan , Song Shuai
Published: September 2024

Bridges are prone to damage from various factors, impacting the overall safety of transportation networks. Accurate damage identification is crucial for maintaining bridge integrity. This study proposes a novel method using encoded images and a convolutional neural network (CNN) for bridge damage identification. By converting raw acceleration data into encoded images, the data can be represented from multiple perspectives, enhancing the extraction of essential features related to bridge damage states. The method was validated using data simulated from a continuous rigid-frame bridge model. The results demonstrate that using encoded images as inputs yields a higher recall rate, precision, and F1-score compared to using acceleration responses as inputs, achieving a comprehensive accuracy of 92%. This study concludes that the combination of encoded images and CNN provides a robust approach for accurate and efficient bridge damage identification.
Non-Woven Fabric Filter Materials Used in Public Buildings for Filtering Particulate Matter Experience Performance Changes under Ultrasonic Cleaning Based on Dual Carbon Target
Authors: Xue Tao , Zhang Xin , Cheng Ping , Sun Fenggang , Liu Fuquan , Yu Tao
Published: September 2024

The long-term use of air filters causes dust to accumulate on their surfaces or fill in the fibers of their internal filtering materials over time, thereby greatly reducing their effectiveness; therefore, the cleaning and replacement of air filter materials in public buildings are of great concern. The most commonly used non-woven fabric materials currently on the market are taken as the research object for this work, in which experiments are conducted on ultrasonic cleaning parameters, cleaning performance, regeneration effects, etc. The results showed that the best cleaning performance was achieved when the ultrasonic cleaning parameter was set to 120 W, and the cleaning time to 11.2 s, with a significant effect on particulate matter with a particle size of less than 2.5 μm. Under the premise of repeated dust containment, when PM10, PM2.5, and PM1.0 are cleaned nine, six, and seven times, respectively, the filtration performance for these particles can also recover to over 85% of the performance of the fresh filter material before cleaning; however, after ten ultrasonic cleanings, the filtration resistance decreased within the range of 4.6~20.7 Pa. In this study, we aim to effectively reduce the replacement of old filters and the generation of incineration pollution, thereby reducing carbon-dioxide-equivalent emissions and providing a reference value for achieving the efficient utilization of filter materials in public buildings under the dual carbon target.
A Deep Insight into the Micro-Mechanical Properties of Mortar through a Multi-Phase Model
Authors: Li Qiang , Huang Jin , Tang Chao , Meng Lingfeng , Yu Yanyan , Wei Kaiyuan
Published: September 2024

This study investigates the micro-mechanical behavior of mortar under uniaxial compression using a three-phase model in PFC3D. By simulating mortar as a composite of cement, sand, and the interfacial transition zone (ITZ), the research examines the impact of particle size on stress–strain behavior, crack propagation, porosity distribution, contact forces, and energy transformation. The simulations reveal that reducing sand particle size from 1–2 mm to 0.25–0.5 mm leads to a significant increase in uniaxial compressive strength, with peak strength values rising from 65.3 MPa to 89.6 MPa. The elastic modulus similarly improves by approximately 20% as particle size decreases. The study also finds that tensile cracks dominate failure, accounting for over 95% of total cracks, with their onset occurring at lower strains as the particle size is reduced. Porosity analysis shows that smaller particles result in a more uniform distribution, with the final porosity at peak strength ranging between 0.26 and 0.29, compared to 0.22 to 0.31 for larger particles. Additionally, energy dissipation patterns reveal that as particle size decreases, the boundary energy transformation into strain energy becomes more efficient, with a 15% increase in strain energy storage observed. These findings provide critical insights into optimizing mortar microstructure for enhanced mechanical performance in construction applications.
The Role of Frailty in the Treatment of Locally Advanced Rectal Cancer
Authors: Stępień Grzegorz J. , Włodarczyk Jakub , Maryńczak Kasper , Prusisz Mateusz , Porc Mateusz , Włodarczyk Marcin , Waśniewska-Włodarczyk Anna , Dziki Łukasz
Published: September 2024

Owing to the gradual aging of today’s population, an increase in the prevalence of frailty syndrome has been noticed. This complex state of health, characterized by decreased resilience and tolerance with concurrent increased vulnerability to stressors and adverse health-related factors, has drawn researchers’ attention in recent years. Rectal cancer, which constitutes ~30% of all colorectal cancers, is a disease noticeably related to the elderly. In its locally advanced form, it is conventionally treated with trimodal therapy—neoadjuvant chemoradiotherapy followed by total mesorectal excision and adjuvant chemotherapy. Despite its good clinical outcomes and improvement in rectal cancer local control, as evidenced by clinical trials, it remains unclear if all frail patients benefit from that approach since it may be associated with adverse side effects that cannot be handled by them. As old patients, and frail ones even more noticeably, are poorly represented in the clinical trials describing outcomes of the standard treatment, this article aims to review the current knowledge on the trimodal therapy of rectal cancer with an emphasis on novel approaches to rectal cancer that can be implemented for frail patients.
Blood–Brain Barrier Conquest in Glioblastoma Nanomedicine: Strategies, Clinical Advances, and Emerging Challenges
Authors: Duan Mengyun , Cao Ruina , Yang Yuan , Chen Xiaoguang , Liu Lian , Ren Boxu , Wang Lingzhi , Goh Boon-Cher
Published: September 2024

Glioblastoma (GBM) is a prevalent type of malignancy within the central nervous system (CNS) that is associated with a poor prognosis. The standard treatment for GBM includes the surgical resection of the tumor, followed by radiotherapy and chemotherapy; yet, despite these interventions, overall treatment outcomes remain suboptimal. The blood–brain barrier (BBB), which plays a crucial role in maintaining the stability of brain tissue under normal physiological conditions of the CNS, also poses a significant obstacle to the effective delivery of therapeutic agents to GBMs. Recent preclinical studies have demonstrated that nanomedicine delivery systems (NDDSs) offer promising results, demonstrating both effective GBM targeting and safety, thereby presenting a potential solution for targeted drug delivery. In this review, we first explore the various strategies employed in preclinical studies to overcome the BBB for drug delivery. Subsequently, the results of the clinical translation of NDDSs are summarized, highlighting the progress made. Finally, we discuss potential strategies for advancing the development of NDDSs and accelerating their translational research through well-designed clinical trials in GBM therapy.
Radiation Therapy in the Management of Head and Neck Mucosal Melanoma
Authors: Azem Omar , Nabulsi Omar , Jelinek Michael , Joshi Nikhil
Published: September 2024

Mucosal melanoma of the head and neck (HNMM) is a rare but highly aggressive malignancy, often diagnosed at an advanced stage with poor prognosis. This review discusses current treatment strategies, emphasizing the role of radiotherapy in managing this challenging disease. A comprehensive analysis of 33 studies provides updated information on techniques and outcomes, highlighting the consistent benefit of adjuvant radiation in improving local control. Advances in conformal techniques, such as intensity-modulated radiotherapy (IMRT), have significantly reduced toxicity rates. Preliminary data on proton and carbon ion therapies suggest the potential for further enhancement of the therapeutic ratio, despite limited availability. Although recent studies report 3-year local control rates as high as 90%, overall survival within the same time frame remains well below 50–60%, underscoring the need for continued improvement in systemic therapies to address the persistent issue of distant metastases.
Using Deauville Scoring to Guide Consolidative Radiotherapy in Diffuse Large B-Cell Lymphoma
Authors: Yau Chun En , Low Chen Ee , Ong Whee Sze , Khoo Lay Poh , Hoe Joshua Tian Ming , Tan Ya Hwee , Chang Esther Wei Yin , Yang Valerie Shiwen , Poon Eileen Yi Ling , Chan Jason Yongsheng , Sin Iris Huili , Yeoh Kheng Wei , Somasundaram Nagavalli , Harunal Rashid Mohamed Farid Bin , Tao Miriam , Lim Soon Thye , Chiang Jianbang
Published: September 2024

Background: The most common aggressive lymphoma in adults is diffuse large B-cell lymphoma (DLBCL). Consolidative radiotherapy (RT) is often administered to DLBCL patients but guidelines remain unclear, which could lead to unnecessary RT. We aimed to evaluate the value of end-of-treatment PET-CT scans, interpreted using the Deauville score (DV), to guide the utilization of consolidative RT, which may help spare low-risk DLBCL patients from unnecessary RT. Methods: We included all DLBCL patients diagnosed between 2010 and 2022 at the National Cancer Centre Singapore with DV measured at the end of the first-line chemoimmunotherapy. The outcome measure was time-to-progression (TTP). The predictive value of DV for RT was assessed based on the interaction effect between the receipt of RT and DV in Cox regression models. Results: The data of 349 patients were analyzed. The median follow-up time was 38.1 months (interquartile range 34.0–42.3 months). RT was associated with a significant improvement in TTP amongst the DV4-5 patients (HR 0.33; 95%CI 0.13–0.88; p = 0.027) but not the DV1-3 patients (HR 0.85; 95%CI 0.40–1.81; p = 0.671) (interaction’s p = 0.133). Multivariable analysis reported that RT was again significantly associated with improved TTP among the DV4-5 patients (adjusted HR 0.29; 95%CI 0.10–0.80; p = 0.017) but not the DV1-3 group (HR 0.86; 95%CI 0.40–1.86; p = 0.707) (interaction’s p = 0.087). Conclusion: Our results suggests that DLBCL patients with end-of-treatment PET-CT DV1-3 may not need consolidative RT. Longer follow-up and prospective randomized trials are still necessary to investigate long-term outcomes.
Layered Double Hydroxide-Based Composites for Concerted Decontamination of Water
Authors: Al Hasnawi Qays , Ion Sabina Gabriela , Tudorache Mădălina , Pavel Octavian Dumitru , Cojocaru Bogdan
Published: September 2024

A series of composites was prepared starting from five types of LDHs, which were then exchanged with three types of metallo-phthalocyanines, and, in the end, magnetic nanoparticles were attached. In the case of LDHs containing Fe, characterization data showed that there was a partial oxidation from Fe2+ to Fe3+. Samples containing evident LDH structures performed better in general than the ones containing iron oxide mixtures, the composites being more active towards amoxicillin removal compared with ampicillin removal. The nature of the phthalocyanine did not have such a great influence, although some differences in the activity were observed. The removal was a combination between adsorption and photocatalytic degradation. The best composites for this application were those based on Mg0.325Fe0.325Al0.25-LDH prepared by co-precipitation in the presence of NaOH and Na2CO3. They presented high adsorption capacity in 10 min and, at the same time, high photocatalytic activity for both amoxicillin and ampicillin.
Utilization of Banana Juice Biomass Waste to Activate CuO/NiO Composites for Electrocatalytic Oxidation of Urea in Alkaline Media
Authors: Naz Irum , Tahira Aneela , Mallah Arfana Begum , Mahar Ihsan Ali , Hayat Asma , Shah Aqeel Ahmed , Dawi Elmuez , AbdElKader Atef , Saleem Lama , Ibrahim Rafat M. , Ibupoto Zafar Hussain
Published: September 2024

The hydrothermal synthesis of CuO/NiO composites was conducted using banana fruit biomass waste. In this study, X-ray powder diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy were used to investigate the crystalline properties, shape structure, and functional group characterization of CuO/NiO composites. The typical morphology of the prepared materials consisted of irregular nanoparticles arranged into clusters of less than 200 nanometers in size. In spite of this, the CuO/NiO composites showed monoclinic CuO and cubic NiO phases and were therefore successfully synthesized. It was observed that rotten banana fruit juice had a significant impact on the particle size and crystal quality of CuO/NiO composites. This was due to the presence of capping, reducing, and stabilizing agents in banana fruit juice. Under alkaline conditions, the CuO/NiO composites were found to be highly electro catalytically active toward the oxidation of urea. Sample 2, which was prepared by adding 1.2 g of CuO decorated with NiO, showed a linear increase in urea detection ranging from 0.1 mM to 17 mM, with a limit of detection of 0.004 mM. Furthermore, sample 2 of the CuO/NiO composite demonstrated exceptional stability, selectivity, and reproducibility. Consequently, sample 2 of CuO/NiO could effectively detect urea in spinach, lotus root, milk, and curd. The improved performance of sample 2 of the CuO/NiO composite can be attributed to its favorable surface properties, which contain enriched active sites and a rapid charge transfer rate.
Synthesis, Characterization, and Attrition Resistance of Kaolin and Boehmite Alumina-Reinforced La0.7Sr0.3FeO3 Perovskite Catalysts for Chemical Looping Partial Oxidation of Methane
Authors: Fotovat Farzam , Beyzaei Mohammad , Ebrahimi Hadi , Mohebolkhames Erfan
Published: September 2024

This study investigates the impact of kaolin and boehmite alumina binders on the synthesis, catalytic properties, and attrition resistance of a La0.7Sr0.3FeO3 (LSF) perovskite catalyst designed for the chemical looping partial oxidation (CLPO) of methane to produce synthesis gas sustainably. The as-synthesized and used catalysts with varying kaolin and boehmite alumina contents (KB(x,y)/LSF) were scrutinized by a variety of characterization methods, including XRD, FE-SEM/EDS, BET, TPD-NH3, and TPD-O2 techniques. The catalytic activity of the synthesized samples was tested at 800 to 900 °C in a fixed-bed reactor producing syngas through the CLPO process over the consecutive redox cycles. Additionally, the attrition resistance of the fresh and used catalyst samples was examined in a jet cup apparatus to assess their durability against the stresses induced by thermal shocks or changes in the crystal lattice caused by chemical reactions. The characterization results showed the pure perovskite crystal structure of KB(x,y)/LSF catalysts demonstrating adequate oxygen adsorption capacity, effective coke mitigation capability, robust thermal stability, and resilience to agglomeration during repetitive redox cycles. Among the tested catalysts, KB(25,15)/LSF was identified as the superior sample, as it could consistently produce syngas with a suitable H2:CO molar ratio varying from 2 to 3 within ten redox cycles at 900 °C, with CH4 conversion and CO selectivity values up to 64% and 87%, respectively. The synthesized catalysts demonstrated a logarithmic attrition pattern in the jet cup tests at room temperature, featuring high attrition resistance after the erosion of particle shape irregularities or weakly bound particles. Moreover, the KB(25,15)/LSF catalyst used at 900 °C showed great resistance in the attrition test, warranting its endurance in the face of extraordinarily harsh conditions in fluidized bed reactors employed for the CLPO process.
The Modulatory Effects and Therapeutic Potential of Cannabidiol in the Gut
Authors: Brown Kevin , Funk Kyle , Figueroa Barrientos Alexa , Bailey Ashly , Shrader Sarah , Feng Wenke , McClain Craig J. , Song Zhao-Hui
Published: September 2024

Cannabidiol (CBD) is a major non-psychotropic phytocannabinoid that exists in the Cannabis sativa plant. CBD has been found to act on various receptors, including both cannabinoid and non-cannabinoid receptors. In addition, CBD has antioxidant effects that are independent of receptors. CBD has demonstrated modulatory effects at different organ systems, such as the central nervous system, immune system, and the gastrointestinal system. Due to its broad effects within the body and its safety profile, CBD has become a topic of therapeutic interest. This literature review summarizes previous research findings with regard to the effect of CBD on the gastrointestinal (GI) system, including its effects at the molecular, cellular, organ, and whole-body levels. Both pre-clinical animal studies and human clinical trials are reviewed. The results of the studies included in this literature review suggest that CBD has significant impact on intestinal permeability, the microbiome, immune cells and cytokines. As a result, CBD has been shown to have therapeutic potential for GI disorders such as inflammatory bowel disease (IBD). Furthermore, through interactions with the gut, CBD may also be helpful in the treatment of disorders outside the GI system, such as non-alcoholic liver disease, postmenopausal disorders, epilepsy, and multiple sclerosis. In the future, more mechanistic studies are warranted to elucidate the detailed mechanisms of action of CBD in the gut. In addition, more well-designed clinical trials are needed to explore the full therapeutic potential of CBD on and through the gut.
The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-N-oxalyl-L-α, β-diaminopropionic acid Limiting Wide Acceptance of the Underutilized Grass Pea (Lathyrus sativus L.)
Authors: Bekele-Alemu Abreham , Girma-Tola Deribew , Ligaba-Osena Ayalew
Published: September 2024

Grass pea (Lathyrus sativus L.) is a protein-rich crop that is resilient to various abiotic stresses, including drought. However, it is not cultivated widely for human consumption due to the neurotoxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) and its association with neurolathyrism. Though some varieties with low β-ODAP have been developed through classical breeding, the β-ODAP content is increasing due to genotype x environment interactions. This review covers grass pea nutritional quality, β-ODAP biosynthesis, mechanism of paralysis, traditional ways to reduce β-ODAP, candidate genes for boosting sulfur-containing amino acids, and the potential and targets of gene editing to reduce β-ODAP content. Recently, two key enzymes (β-ODAP synthase and β-cyanoalanine synthase) have been identified in the biosynthetic pathway of β-ODAP. We proposed four strategies through which the genes encoding these enzymes can be targeted and suppressed using CRISPR/Cas9 gene editing. Compared to its homology in Medicago truncatula, the grass pea β-ODAP synthase gene sequence and β-cyanoalanine synthase showed 62.9% and 95% similarity, respectively. The β-ODAP synthase converts the final intermediate L-DAPA into toxic β-ODAP, whist β-cyanoalanine synthase converts O-Acetylserine into β-isoxazolin-5-on-2-yl-alanine. Since grass pea is low in methionine and cysteine amino acids, improvement of these amino acids is also needed to boost its protein content. This review contains useful resources for grass pea improvement while also offering potential gene editing strategies to lower β-ODAP levels.
Development of Yein-Early, a Unique Fruit-Color and Leaf-Shape Mutant of Citrus unshiu, and Its Specific Selection Marker
Authors: Ko Jung-Gwon , Eun Chang-Ho , Kim In-Jung
Published: September 2024

Citrus unshiu Marc. cv. Miyagawa-wase is one of the most widely cultivated citrus varieties on Jeju Island in Republic of Korea. Mutation breeding is a useful tool for inducing genetic diversity by causing genomic mutations in a short period of time. We previously conducted mutation breeding using gamma irradiation to develop new varieties of C. unshiu. Here, we describe one of these varieties, Yein-early, which has a redder peel, greater hardness, and higher sugar content compared with the wild type (WT). Yein-early leaves also showed a unique phenotype compared with the WT, characterized by longer longitudinal length, shorter transverse length, stronger curling, and longer petiole length. Genome resequencing of Yein-early and the WT uncovered significant single-nucleotide polymorphisms (SNPs) and insertions/deletions (InDels). These variations were crucial in identifying molecular markers unique to Yein-early. In addition, we developed an allele-specific PCR marker specifically targeting a homozygous SNP in Yein-early that distinguishes it from the WT and other citrus varieties. This study contributes to the understanding of pigment synthesis in fruits and provides a valuable tool for selection of the novel Yein-early variety in citrus breeding programs.
Establishment of an Agrobacterium tumefaciens-Mediated Transformation System for Hirsutella sinensis
Authors: Wu Lijuan , Hu Xinkun , Yan Shen , Wu Zenglin , Tang Xuzhong , Xie Lei , Qiu Yujie , Li Rui , Chen Ji , Tian Mengliang
Published: September 2024

Ophiocordyceps sinensis (Berk.) is a complex is formed by Hepialidae larvae and Hirsutella sinensis. Infestation by H. sinensis, interaction with host larvae, and fruiting body development are three crucial processes affecting the formation of O. sinensis. However, research on the molecular mechanism of O. sinensis formation has been hindered by the lack of effective genetic transformation protocols. Therefore, Agrobacterium tumefaciens-mediated transformation (ATMT) was adopted to genetically transform two H. sinensis strains and optimize the transformation conditions. The results revealed that the most suitable Agrobacterium strain for H. sinensis transformation was AGL1, and that the surfactant Triton X-100 could also induce ATMT, although less effectively than acetosyringone (AS). In addition, the endogenous promoters of H. sinensis genes had a stronger ability to drive the expression of the target gene than did the exogenous promoter. The optimal transformation conditions were as follows: AS and hygromycin B concentrations of 100 μM and 50 μg/mL, respectively; A. tumefaciens OD600 of 0.4; cocultivation at 18 °C for 24 h; and H. sinensis used within three passages. The results lay a foundation for the functional study of key regulatory genes involved in the formation of O. sinensis.
Gintonin-Enriched Panax ginseng Extract Fraction Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis through DR4/5 Upregulation
Authors: Hong Seongwoo , Lee Rami , Park Gyun Seok , Han Sumin , Shin Juhyun , Lee Yoon-Mi , Nah Seung-Yeol , Oh Jae-Wook
Published: September 2024

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising chemotherapeutic agent because of its selective apoptotic action on cancer cells. However, resistance to TRAIL-induced apoptosis remains a challenge in many cancers. The gintonin-enriched Panax ginseng extract fraction (GEF) has diverse pharmacological benefits. We explored the combined efficacy of GEF and TRAIL in inducing apoptosis in human renal cell carcinoma (RCC) cells. The effect of GEF treatment on the viability, clonogenic potential, wound healing, and TRAIL-induced apoptotic signaling of RCC cells was studied in vitro. Our investigation revealed that GEF pre-treatment sensitized RCC cells to TRAIL-induced apoptosis, as evidenced by DNA fragmentation and cell proliferation, colony formation, and migration inhibition. This sensitization was linked to the upregulation of death receptors 4 and 5 and alterations in apoptotic protein expression, notably, the decreased expression of the Mu-2-related death-inducing gene, a novel anti-apoptotic protein. Our findings underscore the necessity of caspase activation for GEF/TRAIL-induced apoptosis using the pan-caspase inhibitor Z-VAD-FMK. This study demonstrates that GEF sensitizes human RCC cells to TRAIL-induced apoptosis by upregulating DR4/5 and modulating apoptotic protein expression. These findings suggest a promising strategy for overcoming TRAIL resistance in cancer therapy and highlight the potential of GEF as a valuable adjunct to TRAIL-based treatments.
Acute Heart Failure in a Young Patient Treated in ICU—Diagnostic Pitfalls
Authors: Surówka Łukasz , Andruszkiewicz Paweł , Budnik Monika , Kowalik Robert , Milner Agnieszka , Zawadka Mateusz
Published: September 2024

Background: Chlamydia pneumoniae and human herpesvirus 6 (HHV-6) are uncommon aetiological agents in respiratory tract infections and are rarely associated with cardiogenic shock. This case report presents a rare instance of severe cardiomyopathy linked to these infections in a 19-year-old Asian female. The case highlights the importance of considering a broad differential diagnosis in acute heart failure, especially in young adults. Case report: The patient was admitted with chest pain and diagnosed with ST-elevation myocardial infarction (STEMI) based on electrocardiography. She subsequently developed heart failure, with a marked reduction in myocardial contractility and a left ventricular ejection fraction (LVEF) of 20%. Treatment included broad-spectrum antibiotics and inotropic support guided by hemodynamic monitoring, leading to clinical improvement. The patient was discharged in a significantly improved condition following a stay in the intensive care unit (ICU). Conclusions: This case emphasizes the importance of considering Takotsubo syndrome in differential diagnoses, especially in ICU patients presenting with cardiogenic shock, to improve outcomes and reduce mortality through timely and appropriate management. Inotropic support, often used in the ICU to treat hypoperfusion, may worsen outcomes in patients with Takotsubo syndrome by exacerbating basal hypercontractility and prolonging the acute phase through catecholamine receptor activation.
3D Optical Wedge and Movable Optical Axis LC Lens
Authors: Wu Qi , Zhang Hongxia , Jia Dagong , Liu Tiegen
Published: September 2024

Current liquid crystal (LC) lenses cannot achieve lossless arbitrary movement of the optical axis without mechanical movement. This article designs a novel bottom electrode through simulation and optimization, which forms a special LC lens with an Archimedean spiral electrode, realizing a 3D LC wedge and an arbitrarily movable LC lens. When only the bottom electrode is controlled, it achieves a maximum beam steering angle of 0.164°, which is nearly an order of magnitude larger than the current design. When the top and bottom electrodes are controlled jointly, a 0.164° movement of the lens optical axis is achieved. With focal length varies, the movement of the optical axis ranges from zero to infinity, and the lens surface remains unchanged during movement. The focus can move in a 3D conical area. When the thickness of the LC layer is 30 μm, the fastest response time reaches only 0.635 s, much faster than now.
Reliability and Validity of Ultrasound in Identifying Anatomical Landmarks for Diagnosing A2 Pulley Ruptures: A Cadaveric Study
Authors: Iruretagoiena Xeber , Schöffl Volker , Balius Ramón , Blasi Marc , Dávila Fernando , Sala-Blanch Xavier , Dorronsoro Asier , de la Fuente Javier
Published: September 2024

Background/Objectives: Rock climbing is becoming more popular, leading to an increased focus on diagnosing and treating related injuries. Finger pulley and flexor tendon injuries are common among climbers, with the A2 pulley being the most frequently affected. High-resolution ultrasound (US) is the preferred method for detecting pulley injuries. This study aimed to determine the reliability and validity of US in identifying anatomical landmarks for diagnosing A2 pulley ruptures. Methods: This study was cross-sectional, involving 36 fingers from 4 cadaver arms. A Canon Aplio i800 US machine was used to measure two anatomical landmarks: the midpoint of the proximal phalanx and the distal edge of the A2 pulley. For the first anatomical landmark, the length of the proximal phalanx (PP distance), and for the second landmark, the distance between the distal edges of the proximal phalanx and the A2 pulley (“A” distance), were measured. Measurements were performed by two sonographers and compared to a digital caliper measurement taken post-cadaver dissection. Observers were blinded during measurements to ensure unbiased results. Results: Overall PP distance measured by US (O1: 37.5 ± 5.3 mm, O2: 37.8 ± 5.4 mm) tended to be shorter than caliper measurements (O3: 39.5 ± 5.5 mm). The differences between sonographers were minimal, but larger when compared to caliper measurements. High reliability for PP distance measurement was observed, especially between sonographers, with an ICC average of 0.99 (0.98, 1.00). However, reliability was lower for the “A” distance, with significant differences between US and caliper measurements. Regarding validity, US measurements were valid when compared to caliper measurements for PP distance, but not as reliable for the “A” due to wider confidence intervals. While US can substitute caliper measurements for PP distance (LR, Y:O2, X:O3, −0.70 (−3.28–1.38), 0.98 (0.93 ± 1.04)), its validity for “A” distance is lower (LR, Y:O2, X:O3, −2.37 (−13.53–4.83), 1.02 (0.62–1.75)). Conclusions: US is a reliable and valid tool in identifying anatomical landmarks for diagnosing A2 pulley ruptures, particularly for detecting the midpoint of the proximal phalanx. This is important to differentiate between complete and partial A2 pulley tears. However, the measurement of the “A” distance requires further refinement. These findings support efforts to standardize US examination protocols and promote consensus in diagnostic methodology, though further research is needed to address the remaining challenges.
Diagnostic Challenges and Pathogenetic Differences in Biomass-Smoke-Induced versus Tobacco-Smoke-Induced COPD: A Comparative Review
Authors: Dutta Joytri , Singh Sabita , Greeshma Mandya V. , Mahesh Padukudru Anand , Mabalirajan Ulaganathan
Published: September 2024

Background: Chronic Obstructive Pulmonary Disease (COPD) is a major global health challenge, primarily driven by exposures to tobacco smoke and biomass smoke. While Tobacco-Smoke-Induced COPD (TSCOPD) has been extensively studied, the diagnostic challenges and distinct pathogenesis of Biomass-Smoke-Induced COPD (BSCOPD), particularly in low- and middle-income countries, remain underexplored. Objective: To explore the differences in clinical manifestations, pulmonary function, and inflammatory profiles between BSCOPD and TSCOPD and highlight the diagnostic complexities of BSCOPD. Methods: This review analyzes the current literature comparing BSCOPD with TSCOPD, focusing on distinctive pathophysiological mechanisms, inflammatory markers, and oxidative stress processes. Results: BSCOPD presents differences in clinical presentation, with less emphysema, smaller airway damage, and higher rates of pulmonary hypertension compared to TSCOPD. BSCOPD is also characterized by bronchial hyperresponsiveness and significant hypoxemia, unlike TSCOPD, which exhibits severe airflow obstruction and emphysema. Additionally, the inflammatory profile of BSCOPD includes distinct mucous hypersecretion and airway remodeling. Conclusions: The unique genetic, epigenetic, and oxidative stress mechanisms involved in BSCOPD complicate its diagnosis and management. Biomass smoke’s underrecognized impact on accelerated lung aging and exacerbation mechanisms emphasizes the need for targeted research to refine diagnostic criteria and management strategies for BSCOPD. Future directions: Further research should focus on identifying specific biomarkers and molecular pathways to enhance early diagnosis and improve clinical outcomes in populations exposed to biomass smoke.
Monitoring Gas Emissions in Agricultural Productions through Low-Cost Technologies: The POREM (Poultry-Manure-Based Bio-Activator for Better Soil Management through Bioremediation) Project Experience
Authors: Suriano Domenico , Abulude Francis Olawale
Published: September 2024

Agricultural production or rural activities can involve the emission of unpleasant gases, malodors, or most commonly, greenhouse gases. In any case, the control and monitoring of such emissions in rural, unattended, and remote locations represent an issue in need of addressing. In this article, the monitoring of gases produced by a poultry manure depot and performed by devices based on low-cost gas sensors in the context of the POREM (poultry-manure-based bio-activator for better soil management through bioremediation) project is reported. This experience has shown that the continuous and real-time monitoring of gas emissions in an unattended, remote, and rural area, where it is unfeasible to employ expensive, professional instruments, can be successfully performed by low-cost technologies. Two portable monitoring units developed in the laboratory and based on low-cost gas sensors were used to provide indications about the concentrations of NH3, CH4, H2S, and CO2. During this experiment, two monitors were deployed: the first one was placed in the manure storage depot, while the second one was deployed out of the storage site to compare the gas concentrations related to the outdoor environment with the gas emissions coming from the manure. Both devices were wirelessly linked to the Internet, even though the radio signal was weak and unstable in that area. This situation provided us with the opportunity to test a particular protocol based on sending and receiving e-mails containing commands for the remote machines. This experiment proved the effectiveness of the use of low-cost devices for gas emission monitoring in such particular environments.
How Old Is a Turtle? Challenges in Interpreting Age Information in Sea Turtles
Authors: Morales-Mérida B. Alejandra , Pilcher Nicolas J. , Girondot Marc
Published: September 2024

Marine turtles are iconic, globally distributed large reptiles with a largely oceanic life history that makes it difficult to characterize population demography and dynamics. This is significant because all marine turtle species are currently considered vulnerable or endangered. Knowledge of the age of individuals is central to our understanding of the life history of a species and an important consideration in the design of management and conservation strategies. Herein, we review different methods used to estimate the absolute, relative and physiological age of marine turtles, as well as their underlying hypotheses and challenges in their interpretation. We conclude that, at present, there is no validated method that establishes the absolute age of an individual from field studies.
Collaboration Skills and Puzzles: Development of a Performance-Based Assessment—Results from 12 Primary Schools in Greece
Authors: Demetroulis Emmanouil A. , Papadogiannis Ilias , Wallace Manolis , Poulopoulos Vassilis , Theodoropoulos Anastasios , Vasilopoulos Nikos , Antoniou Angeliki , Dasakli Fotini
Published: September 2024

Collaboration skills are essential for the effectiveness and efficiency of collective efforts in both professional and personal contexts. However, their complex and intertwined nature poses challenges in both defining and assessing them. To develop educational methodologies aimed at enhancing the cultivation of collaboration skills, there is a need for developing pre-post experimental assessment tools that target the construct in real-life school settings. Research indicates a lack of performance-based assessment tools designed to assess collaboration skills. This research proposes a performance-based assessment developed through collecting evidence from individuals participating in both solo and group puzzle activities. The assessment is created by viewing collaboration skills through the lens of the ATC21S framework. A total of 148 students aged from 11 to 12 years old from 12 different public schools in Greece participated in this study, carried out over a period of 7 months between December 2023 and June 2024 in both the test and retest phases. The results show that, regardless of the group compositions, individual student collaborative performance was stable. The validity of using Spearman’s correlation coefficient was measured at 0.623.
Sustainable Early English Language Education: Exploring the Content Knowledge of Six Chinese Early Childhood Education Teachers Who Teach English as a Foreign Language
Authors: Shi Xiaobo , Yeung Susanna Siu-sze
Published: September 2024

The importance of content knowledge (CK) for effective teaching and student learning is widely recognized. However, there is still a lack of detailed understanding of the CK held by early childhood education (ECE) teachers, particularly those teaching English as a foreign language (EFL). To fill this gap, our study utilized a qualitative approach, specifically stimulated recall classroom observation, to examine the various categories and subcategories of CK among Chinese EFL teachers in kindergartens. Six EFL teachers participated, two with English backgrounds and four with ECE backgrounds. Our findings highlight the multifaceted nature of CK in ECE EFL teaching, identifying three categories: knowledge of first language (L1) acquisition, knowledge of second language (L2) acquisition, and knowledge of linguistics. Notably, there were significant differences between the teachers with English and ECE majors, particularly in the most frequently mentioned CK subcategory and in how L1 is learned. This study not only illuminates these differences but also provides valuable insights for future research and practical applications in ECE EFL teacher education.
Vulnerable Narcissism and Emotion Dysregulation as Mediators in the Link between Childhood Emotional Abuse and Binge Watching
Authors: Verrastro Valeria , Calaresi Danilo , Giordano Fiorenza , Saladino Valeria
Published: September 2024

Individuals who have faced childhood emotional abuse (CEA) may develop vulnerable narcissistic tendencies and experience difficulties in regulating their emotions. These psychological vulnerabilities can contribute to the adoption of binge watching as a maladaptive coping mechanism. The present study aimed to investigate the potential mediating role of vulnerable narcissism and emotion dysregulation in the relationship between CEA and binge watching. Additionally, this study examined whether this model was gender-invariant. A sample of 1082 emerging adults, aged 18 to 25 (50% women), completed self-report questionnaires. The results revealed significant direct and indirect relationships among all the variables of interest, and the model was found to be invariant across genders. Notably, both men and women who experienced CEA and exhibited higher levels of vulnerable narcissism and emotion dysregulation reported engaging in higher levels of binge watching. These findings highlight the importance of the early identification of CEA, the implementation of targeted interventions, and the provision of trauma-informed care to address the negative consequences of CEA and mitigate the development of binge-watching behaviors. Moreover, the results emphasize the need for tailored prevention and intervention programs that address vulnerable narcissism and emotion dysregulation as potential pathways to inform effective therapeutic strategies.
Unlocking Success in Counseling: How Personality Traits Moderates Its Effectiveness
Authors: Fortunato Alexandro , Andreassi Silvia , Franchini Costanza , Sciabica Gaetano Maria , Morelli Mara , Chirumbolo Antonio , Speranza Anna Maria
Published: September 2024

Psychological distress is widespread among university students, with depression being notably more prevalent compared to the general population. University counseling services are crucial for addressing these mental health challenges, and numerous studies have demonstrated their effectiveness in reducing psychological distress and improving overall well-being. However, there is limited research on what factors predict the success of university counseling. This study aims to evaluate whether counseling improves well-being, specifically by reducing depressive symptoms, and to explore whether personality traits influence counseling outcomes. Participants included 125 Italian university students (64.8% female, mean age = 22.69; SD = 3.04) who utilized counseling services. They completed a socio-demographic questionnaire, the Beck Depression Inventory-II (BDI-II), and the Personality Inventory for DSM-5-TR (PID-5-TR) at three points: immediately after the intake interview (T0), just before the intervention (T1), and after the fourth session (T2). Linear mixed models were used to analyze changes in depression levels, revealing a significant reduction in depressive symptoms from pre- to post-intervention. Among personality traits, only antagonism showed a significant interaction with time. Additionally, higher detachment scores were associated with higher depression levels. These findings emphasize the need for focused attention on students’ emotional issues and suggest that personality traits may influence the effectiveness of counseling.
Automated Classification and Segmentation and Feature Extraction from Breast Imaging Data
Authors: Sun Yiran , Zhu Zede , Honarvar Shakibaei Asli Barmak
Published: September 2024

Breast cancer is the most common type of cancer in women and poses a significant health risk to women globally. Developments in computer-aided diagnosis (CAD) systems are focused on specific tasks of classification and segmentation, but few studies involve a completely integrated system. In this study, a comprehensive CAD system was proposed to screen ultrasound, mammograms and magnetic resonance imaging (MRI) of breast cancer, including image preprocessing, breast cancer classification, and tumour segmentation. First, the total variation filter was used for image denoising. Second, an optimised XGBoost machine learning model using EfficicnetB0 as feature extraction was proposed to classify breast images into normal and tumour. Third, after classifying the tumour images, a hybrid CNN deep learning model integrating the strengths of MobileNet and InceptionV3 was proposed to categorise tumour images into benign and malignant. Finally, Attention U-Net was used to segment tumours in annotated datasets while classical image segmentation methods were used for the others. The proposed models in the designed CAD system achieved an accuracy of 96.14% on the abnormal classification and 94.81% on tumour classification on the BUSI dataset, improving the effectiveness of automatic breast cancer diagnosis.