bluuebunny/crossref_metadata_2025 huggingface dataset:
Remnant cholesterol is associated with cardiovascular mortality
Authors: Zhang Kerui , Qi Xiangyun , Zhu Fuyu , Dong Quanbin , Gou Zhongshan , Wang Fang , Xiao Li , Li Menghuan , Chen Lianmin , Wang Yifeng , Zhang Haifeng , Sheng Yanhui , Kong Xiangqing
Published: September 2022

Background

Genetic, observational, and clinical intervention studies indicate that circulating levels of remnant cholesterol (RC) are associated with cardiovascular diseases. However, the predictive value of RC for cardiovascular mortality in the general population remains unclear.

Methods

Our study population comprised 19,650 adults in the United States from the National Health and Nutrition Examination Survey (NHANES) (1999–2014). RC was calculated from non-high-density lipoprotein cholesterol (non-HDL-C) minus low-density lipoprotein cholesterol (LDL-C) determined by the Sampson formula. Multivariate Cox regression, restricted cubic spline analysis, and subgroup analysis were applied to explore the relationship of RC with cardiovascular mortality.

Results

The mean age of the study cohort was 46.4 ± 19.2 years, and 48.7% of participants were male. During a median follow-up of 93 months, 382 (1.9%) cardiovascular deaths occurred. In a fully adjusted Cox regression model, log RC was significantly associated with cardiovascular mortality [hazard ratio (HR) 2.82; 95% confidence interval (CI) 1.17–6.81]. The restricted cubic spline curve indicated that log RC had a linear association with cardiovascular mortality (p for non-linearity = 0.899). People with higher LDL-C (≥130 mg/dL), higher RC [≥25.7/23.7 mg/dL in males/females corresponding to the LDL-C clinical cutoff point (130 mg/dL)] and abnormal HDL-C (<40/50 mg/dL in males/females) levels had a higher risk of cardiovascular mortality (HR 2.18; 95% CI 1.13–4.21 in males and HR 2.19; 95% CI 1.24–3.88 in females) than the reference group (lower LDL-C, lower RC and normal HDL-C levels).

Conclusions

Elevated RC levels were associated with cardiovascular mortality independent of traditional risk factors.
The emerging multifunctional roles of ERAP1, ERAP2 and IRAP between antigen processing and renin-angiotensin system modulation
Authors: Mattorre Benedetta , Tedeschi Valentina , Paldino Giorgia , Fiorillo Maria Teresa , Paladini Fabiana , Sorrentino Rosa
Published: September 2022

The Endoplasmic Reticulum Aminopeptidase 1 and 2 (ERAP1 and ERAP2) and Insulin Regulated Aminopeptidase (IRAP) are three M1 zinc metalloproteases whose role in antigen processing is the refining of peptidome either in the Endoplasmic reticulum (ERAP1 and ERAP2), or in the endosomes (IRAP). However, other novel and distinct functions are emerging. Here, we focus specifically on ERAP2. This gene has a peculiar evolutionary history, being absent in rodents and undergoing in humans to a balanced selection of two haplotypes, one of which not expressing the full length ERAP2. These observations suggest that its role in antigen presentation is not essential. An additional, less investigated role is in the regulation of the Renin Angiotensin System (RAS). ERAP1 and ERAP2 cleave Angiotensin II (Ang II) into Ang III and IV, which counteract the action of Ang II whereas IRAP is itself the receptor for Ang IV. We have recently reported that macrophages, independently from the haplotype, express and release a N-terminus ERAP2 “short” form which directly binds IRAP and the two molecules are co-expressed in the endosomes and on the cell membrane. This new evidence suggests that the maintenance of the ERAP2 gene in humans could be due to its activity in the regulation of the RAS system, possibly as an Ang IV agonist. Its role in the immune-mediated diseases as well as in disorders more specifically related to an imbalance of the RAS system, including hypertension, pre-eclampsia but also viral infections such as COVID-19, is discussed here.
Transcriptional programming of immunoregulatory responses in human Langerhans cells
Authors: Davies James , Sirvent Sofia , Vallejo Andres F. , Clayton Kalum , Douilhet Gemma , Keeler Patrick S. , West Jonathan , Ardern-Jones Michael , MacArthur Ben D. , Singh Harinder , Polak Marta E.
Published: September 2022

Human epidermal Langerhans cells (LCs) maintain immune homeostasis in the skin. To examine transcriptional programming of human primary LCs during homeostasis, we performed scRNA-seq analysis of LCs before and after migration from the epidermis, coupled with functional assessment of their regulatory T cell priming capabilities. The analysis revealed that steady-state LCs exist in a continuum of maturation states and upregulate antigen presentation genes along with an immunoregulatory module including the genes IDO1, LGALS1, LAMTOR1, IL4I, upon their migration. The migration-induced transition in genomic state is accompanied by the ability of LCs to more efficiently prime regulatory T cell responses in co-culture assays. Computational analyses of the scRNAseq datasets using SCENIC and Partial Information Decomposition in Context identified a set of migration-induced transcription factors including IRF4, KLF6 and RelB as key nodes within a immunoregulatory gene regulatory network. These findings support a model in which efficient priming of immunoregulatory responses by LCs is dependent on coordinated upregulation of a migration-coupled maturation program with a immunoregulation-promoting genomic module.
Common variable immunodeficiency in two kindreds with heterogeneous phenotypes caused by novel heterozygous NFKB1 mutations
Authors: Staels Frederik , De Keukeleere Kerstin , Kinnunen Matias , Keskitalo Salla , Lorenzetti Flaminia , Vanmeert Michiel , Prezzemolo Teresa , Pasciuto Emanuela , Lescrinier Eveline , Bossuyt Xavier , Gerbaux Margaux , Willemsen Mathijs , Neumann Julika , Van Loo Sien , Corveleyn Anniek , Willekens Karen , Stalmans Ingeborg , Meyts Isabelle , Liston Adrian , Humblet-Baron Stephanie , Seppänen Mikko , Varjosalo Markku , Schrijvers Rik
Published: September 2022

NFKB1 haploinsufficiengcy was first described in 2015 in three families with common variable immunodeficiency (CVID), presenting heterogeneously with symptoms of increased infectious susceptibility, skin lesions, malignant lymphoproliferation and autoimmunity. The described mutations all led to a rapid degradation of the mutant protein, resulting in a p50 haploinsufficient state. Since then, more than 50 other mutations have been reported, located throughout different domains of NFKB1 with the majority situated in the N-terminal Rel homology domain (RHD). The clinical spectrum has also expanded with possible disease manifestations in almost any organ system. In silico prediction tools are often used to estimate the pathogenicity of NFKB1 variants but to prove causality between disease and genetic findings, further downstream functional validation is required. In this report, we studied 2 families with CVID and two novel variants in NFKB1 (c.1638-2A>G and c.787G>C). Both mutations affected mRNA and/or protein expression of NFKB1 and resulted in excessive NLRP3 inflammasome activation in patient macrophages and upregulated interferon stimulated gene expression. Protein-protein interaction analysis demonstrated a loss of interaction with NFKB1 interaction partners for the p.V263L mutation. In conclusion, we proved pathogenicity of two novel variants in NFKB1 in two families with CVID characterized by variable and incomplete penetrance.
Topological states in boron phosphide with zinc-blende structure
Authors: Li Yang
Published: September 2022

The field of topological states in phonon of solids have been rapidly developing in recent years. This work examined the phonon dispersion of a compound Boron Phosphide (BP) with a Zinc-Blende structure via first-principle calculation. The results show that BP is a stable compound in theory and hosts rich topological signatures in its phonon dispersion. Specifically, Weyl and quadratic nodal line states can be found in the acoustic branches, and triple point and quadratic contact triple point can be found in the optical branches. It is hoped that the rich topological states in BP can be imaged by inelastic x-ray scattering or neutron scattering in the near future.
Quantitative evaluation of the influence of multiple MRI sequences and of pathological tissues on the registration of longitudinal data acquired during brain tumor treatment
Authors: Canalini Luca , Klein Jan , Waldmannstetter Diana , Kofler Florian , Cerri Stefano , Hering Alessa , Heldmann Stefan , Schlaeger Sarah , Menze Bjoern H. , Wiestler Benedikt , Kirschke Jan , Hahn Horst K.
Published: September 2022

Registration methods facilitate the comparison of multiparametric magnetic resonance images acquired at different stages of brain tumor treatments. Image-based registration solutions are influenced by the sequences chosen to compute the distance measure, and the lack of image correspondences due to the resection cavities and pathological tissues. Nonetheless, an evaluation of the impact of these input parameters on the registration of longitudinal data is still missing. This work evaluates the influence of multiple sequences, namely T1-weighted (T1), T2-weighted (T2), contrast enhanced T1-weighted (T1-CE), and T2 Fluid Attenuated Inversion Recovery (FLAIR), and the exclusion of the pathological tissues on the non-rigid registration of pre- and post-operative images. We here investigate two types of registration methods, an iterative approach and a convolutional neural network solution based on a 3D U-Net. We employ two test sets to compute the mean target registration error (mTRE) based on corresponding landmarks. In the first set, markers are positioned exclusively in the surroundings of the pathology. The methods employing T1-CE achieves the lowest mTREs, with a improvement up to 0.8 mm for the iterative solution. The results are higher than the baseline when using the FLAIR sequence. When excluding the pathology, lower mTREs are observable for most of the methods. In the second test set, corresponding landmarks are located in the entire brain volumes. Both solutions employing T1-CE obtain the lowest mTREs, with a decrease up to 1.16 mm for the iterative method, whereas the results worsen using the FLAIR. When excluding the pathology, an improvement is observable for the CNN method using T1-CE. Both approaches utilizing the T1-CE sequence obtain the best mTREs, whereas the FLAIR is the least informative to guide the registration process. Besides, the exclusion of pathology from the distance measure computation improves the registration of the brain tissues surrounding the tumor. Thus, this work provides the first numerical evaluation of the influence of these parameters on the registration of longitudinal magnetic resonance images, and it can be helpful for developing future algorithms.
Decoding competitive endogenous RNA regulatory network in postoperative cognitive dysfunction
Authors: Wang Wei , Huo Pengwei , Zhang Lei , Lv Gang , Xia Zhongyuan
Published: September 2022

Postoperative cognitive dysfunction (POCD) is a common postoperative neurological complication in elderly patients. Circular RNAs (circRNAs) are abundant in the mammalian brain and can probably regulate cognitive function. However, the competitive endogenous RNA (ceRNA) regulatory network in POCD remains illiterate. Transcriptomic signatures in the hippocampus of POCD mice derived from the Gene Expression Omnibus (GEO) dataset GSE190880, GSE95070, and GSE115440 were used to identify the circRNA, miRNA, and mRNA expression profiles of POCD mice compared with controls, respectively. A set of differentially expressed RNAs, including 119 circRNAs, 33 miRNAs, and 49 mRNAs were identified. Transcript validation showed the enhanced expression of circ_0001634, circ_0001345, and circ_0001493. A ceRNA regulatory network composed of three circRNAs, three miRNAs, and six mRNAs was established. The hub mRNAs in the ceRNA network were further found to be involved in the hormone catabolic process and regulation of canonical Wnt signaling pathway, revealing their crucial role in POCD. Finally, three miRNAs and four mRNAs were verified by qRT-PCR. These results based on bioinformatics and PCR array suggest that circ_0001634/miR-490-5p/Rbm47, circ_0001634/miR-490-5p/Sostdc1, circ_0001634/miR-7001-5p/Sostdc1, circ_0001345/miR-7001-5p/Sostdc1, and circ_0001493/miR-7001-5p/Sostdc1 may be novel diagnostic biomarkers and therapeutic targets for POCD.
Identification of therapeutically potential targets and their ligands for the treatment of OSCC
Authors: Kumari Pratima , Kumar Sugandh , Sethy Madhusmita , Bhue Shyamlal , Mohanta Bineet Kumar , Dixit Anshuman
Published: September 2022

Recent advancements in cancer biology have revealed molecular changes associated with carcinogenesis and chemotherapeutic exposure. The available information is being gainfully utilized to develop therapies targeting specific molecules involved in cancer cell growth, survival, and chemoresistance. Targeted therapies have dramatically increased overall survival (OS) in many cancers. Therefore, developing such targeted therapies against oral squamous cell carcinoma (OSCC) is anticipated to have significant clinical implications. In the current work, we have identified drug-specific sensitivity-related prognostic biomarkers (BOP1, CCNA2, CKS2, PLAU, and SERPINE1) using gene expression, Cox proportional hazards regression, and machine learning in OSCC. Dysregulation of these markers is significantly associated with OS in many cancers. Their elevated expression is related to cellular proliferation and aggressive malignancy in various cancers. Mechanistically, inhibition of these biomarkers should significantly reduce cellular proliferation and metastasis in OSCC and should result in better OS. It is pertinent to note that no effective small-molecule candidate has been identified against these biomarkers to date. Therefore, a comprehensive in silico drug design strategy assimilating homology modeling, extensive molecular dynamics (MD) simulation, and ensemble molecular docking has been applied to identify potential compounds against identified targets, and potential molecules have been identified. We hope that this study will help in deciphering potential genes having roles in chemoresistance and a significant impact on OS. It will also result in the identification of new targeted therapeutics against OSCC.
Construction of immune cell infiltration protein network based on clinical low grade glioma cases
Authors: Jiang Wei , He Zijian , Jiang Weizhong , Du Jiarui , Yuan Lutao , Luo Cong , Li Xiang , Xu Fulin
Published: September 2022

Many researchers have studied low-grade glioma and the immune microenvironment have been studied by many researchers. Recent studies suggest that macrophages and dendritic cells trigger part of the local immune dysregulation in the tumor microenvironment, and they have been polarized into a mixed pro-inflammatory and immunosuppressive phenotype. It is suggested that the degree of immune infiltration is related to the survival, therapeutic effect, and prognosis of patients. This opens up new avenues for cancer treatment. On the basis of immune infiltration degree, a protein interaction network (PIN) and a prognosis model were established, and we chose the top 20 pathways from enrichment analysis to provide potential targets for glioma clinical treatment.
Changes in soil bacterial community and functions by substituting chemical fertilizer with biogas slurry in an apple orchard
Authors: Zhang He , Ma Yue , Shao Jianzhu , Di Rui , Zhu Feng , Yang Zhichang , Sun Jianshe , Zhang Xueying , Zheng Chunyan
Published: September 2022

Growing concerns about the negative environmental effects of excessive chemical fertilizer input in fruit production have resulted in many attempts looking for adequate substitution. Biogas slurry as a representative organic fertilizer has the potential to replace chemical fertilizer for improvement of sustainability. However, it is still poorly known how biogas slurry applications may affect the composition of soil microbiome. Here, we investigated different substitution rates of chemical fertilizer with biogas slurry treatment (the control with no fertilizer and biogas slurry, CK; 100% chemical fertilizer, CF; biogas slurry replacing 50% of chemical fertilizer, CBS; and biogas slurry replacing 100% of chemical fertilizer, BS) in an apple orchard. Soil bacterial community and functional structure among treatments were determined using Illumina sequencing technology coupled with Functional Annotation of Prokaryotic Taxonomy (FAPROTAX) analysis. Leaf nutrient contents, apple fruit and soil parameters were used to assess plant and soil quality. Results showed that most of fruit parameters and soil properties were significantly varied in the four treatments. CBS treatment increased the contents of soil organic matter, alkali nitrogen and available potassium average by 49.8%, 40.7% and 27.9%, respectively. Treatments with biogas slurry application increased the single fruit weight, fresh weight, and dry weight of apple fruit average by 15.6%, 18.8% and 17.8, respectively. Soil bacterial community dominance and composition were significantly influenced by substituting of chemical fertilizer with biogas slurry. Biogas slurry application enhanced the relative abundance of some beneficial taxa (e.g. Acidobacteria Gp5 and Gp7, Parasegetibacter) and functional groups related to carbon and nitrogen cycling such as chemoheterotrophy, cellulolysis, and nitrogen fixation. Soil available phosphorus and potassium, pH and electrical conductivity were identified having a high potential for regulating soil bacterial specific taxa and functional groups. This study showed that the proper ratio application (50%: 50%) of biogas slurry with chemical fertilizer could regulate soil bacterial composition and functional structure via changes in soil nutrients. The variations of bacterial community could potentially take significant ecological roles in maintaining apple plant growth, soil fertility and functionality.
Resistance induction and nematicidal activity of certain monoterpenes against tomato root-knot caused by Meloidogyne incognita
Authors: Elsharkawy Mohsen Mohamed , Al-Askar Abdulaziz A. , Behiry Said I. , Abdelkhalek Ahmed , Saleem Muhammad Hamzah , Kamran Muhammad , Derbalah Aly
Published: September 2022

This research was performed to evaluate the potential of carvone, cuminaldehyde, cineole, and linalool for the control of root-knot of tomato. The tested control agents were evaluated for their ability to stimulate systemic resistance toMeloidogyne incognitain tomato by monitoring the transcription levels of defense-related genes. Moreover, the ability of the tested agents to induce nematicidal activity concerning second-stage juveniles (J2) hatching and mortality was evaluated. Furthermore, the effect of the tested agents on certain tomato growth and yield parameters was assessed. The tested monoterpenes showed high nematicidal activity againstM. incognitaconcerning J2 hatching inhibition and mortality. Carvone, cuminaldhyde, linalool, and cineole had LC50values of 123.5, 172.2, 354.9, 466.4, and 952.3 μg/mL, respectively. Carvone was found to be the most efficient hatching inhibitor. The tested monoterpenes showed a high potential against root-knot under greenhouse and field conditions with respect to root-galling, egg masses, and the number of J2. Carvone was the most effective treatment. The growth and yield characters of treated tomato were significantly increased in monoterpenes treatments compared to untreated control. Treated tomato plants showed expression of defense-related genes (PR1andPAL) 5-8 folds higher than the control. The results also showed that cuminaldhyde, followed by carvone, linalool, and cineole, had the greatest levels of expression in tomato plants. Taken together, the selected monoterpenes could be used as alternatives to control the root-knot of tomato.
Aging-friendly cities: Investigating the effects of street usage on the psychological satisfaction of older adults in megacities
Authors: Li Jian , Gao Xing , Qiu Yue , Ling Yantao
Published: September 2022

The psychological satisfaction of older adults is an important evaluation standard for the construction of elder-friendly cities. Meanwhile, as important space carriers carrying the travel activities and social participation of older adults, streets are also of great significance to improve psychological wellbeing. However, few studies pay attention to the street usage of aging population, especially in the context of megacities. Moreover, the previous literature rarely distinguishes the types of streets. Thus, employing a mixed approach, we investigate the relationships between street usage and psychological satisfaction for older adults. Based on the survey in Shanghai, we find that a clear role for different usage indicators in the determination of subjective psychological satisfaction of older adults. More specially, the street usage and psychological satisfaction for older adults are strongly correlated, especially for living streets. Psychological satisfaction for older adults in different types of streets is not always positively related to the positive perception of street usage. The psychological satisfaction of different streets depends on different factors. By focusing on the case of a megacity, our study emphasizes the differences between different types of streets, which will be conducive to the proposal of practical planning policies. In addition, employing mixed research methods not only explains how different street usage affects the psychological welfare of older adults on a macro scale, but also emphasizes the inner world of respondents.
Collaboration among psychological researchers, the government, and non-profit organizations for “Konkatsu” (marriage hunting) in Japan
Authors: Nishimura Takashi , Souma Toshihiko , Kito Mie , Taniguchi Junichi , Kanemasa Yuji , Yamada Junko , Miyagawa Yuki
Published: September 2022

In contemporary Japanese society, it is difficult to find a marriage partner, and therefore, “Konkatsu,” the search for a marriage partner, has become a socially accepted activity in Japan. In response to this social challenge, in addition to private companies, governments and non-profit organizations are supporting individuals in their search for a marriage partner. This paper reviews statistical information related to marriage hunting published in Japan. In addition, some of the authors’ collaborative activities and academic publications based on these activities are reviewed. Subsequently, the paper discusses and highlights the importance of helping individuals have confidence in their physical attractiveness.
Knowing and being known: Psychedelic–assisted psychotherapy and the sense of authenticity
Authors: Fischman Lawrence G.
Published: September 2022

Participants in MDMA- and psychedelic-assisted psychotherapy often emerge from these treatments with new beliefs about themselves and the world. Studies have linked changed beliefs with mystical experiences reported by some participants during drug sessions. While there has been some debate about the epistemic value of drug-induced mystical experiences, and about the need for consent to treatments that may alter metaphysical beliefs, less attention has been given to the sense of authenticity that attends these experiences. In this paper, I consider the intersubjective context in which these changed beliefs arise. I suggest that the sense of authenticity people experience with MDMA- and psychedelic-assisted psychotherapy derives from a simultaneous feeling of knowing and being known. The medications used in these treatments reduce the defensive barriers which ordinarily prevent powerful feelings from being intersubjectively shared, allowing the subject to experience knowing and being known with the therapist and/or internalized or imagined others. In explaining this thesis, I discuss Ratcliffe's “existential feeling;” ipseity in incipient psychosis and psychedelic states; Winnicott's notions of the True Self, omnipotence, creativity, and transitional phenomena; implicit relational knowing and moments of meeting; infant-mother dyad research; predictive processing and the relaxed beliefs model of psychedelic action; the role of the “partner in thought” in knowing and feeling known. I propose that a “transitional space” model of MDMA- and psychedelic-assisted psychotherapy is well-suited for working through “not-me” or dissociated experience
First-episode olfactory hallucination in a patient with anxiety disorder: A case report
Authors: Jiang Xingmei , Yuan Yiwen , Li Zhixiong , Ou Ying , Li Zhe
Published: September 2022

Background

Olfactory hallucination refers to olfactory perception in the absence of chemical stimuli. Although it has been associated with many neurological and psychotic disorders, it has rarely been reported as the first and only symptom in patients with anxiety disorder, and its treatment remains inadequate.

Case summary

A 66-year-old woman who had been experiencing gradually worsening olfactory hallucinations for almost 4 years was diagnosed with generalized anxiety disorder. Olfactory hallucination disappeared after treatment with anti-anxiety drugs.

Conclusion

Olfactory hallucination can be the first and only symptom in patients with anxiety disorder and may be effectively treated with anti-anxiety medication. In fact, it can precede the diagnosis of anxiety disorder by several years.
Determinants of neonatal near-miss among neonates delivered in public hospitals of Ilu Abba Bor Zone, Southwest Ethiopia: An unmatched case–control study during the COVID-19 pandemic
Authors: Debele Gebiso Roba , Siraj Sabit Zenu , Tsegaye Dereje , Temesgen Ermiyas
Published: September 2022

Background

The neonatal period is the time with the highest risk of neonatal and infant mortality. The COVID-19 pandemic diverted resources from routine maternal health services, which raises the possibility of neonatal near misses (NNMs). To implement prompt treatments that could improve the standard of infant care and lower neonatal mortality, it has been theorized that pinpointing the determinants of NNM during this outbreak is crucial. In light of this, the current study identified the determinants of NNM in neonates delivered in public hospitals of Ilu Abba Bor Zone, South West Ethiopia.

Methods

An institution-based unmatched case–control study was conducted among randomly selected 303 (101 cases and 202 controls) neonates admitted to Mettu Karl Comprehensive Specialized Hospital (MKCSH) and Darimu Primary Hospital (DPH) from 1 November to 28 December 2020. Data were collected using interviewer-administered structured questionnaire and checklist. The collected data were coded and entered into Epi-Data version 4.6 and then exported to SPSS version 20 for analysis. Adjusted odds ratios (AOR) along with a 95% confidence interval was used to assess the strength of the association, and a p-value < 0.05 was considered to declare the statistical significance in the multivariable logistic regression analysis.

Result

A total of 303 (101 cases and 202 controls) neonates admitted to MKCSH and DPH were included in the study making a 97.4% response rate. In the multivariable logistic regression analysis, no formal maternal education [AOR = 3.534, 95% CI: (1.194–10.455)], Breech presentation during birth [AOR = 3.088, 95% CI: (1.029–9.268)], < 4 antenatal care (ANC) visits [AOR = 1.920, 95% CI: (1.065–3.461], cesarean section delivery [AOR = 4.347, 95% CI: (1.718–10.996)], antepartum hemorrhage (APH) [AOR = 3.37, 95% CI: (1.23–9.24)], and hypertensive disorders of pregnancy (HDP) [AOR = 4.05, 95% CI: (2.36–11.05)] were independent determinants of NNM.

Conclusion

The study's result revealed that factors such as education level, birth presentation, ANC visit, mode of delivery, APH, and HDP continued to be important determinants of the NNM in Ethiopia during this pandemic. Therefore, much work is needed to improve neonatal health by providing adequate ANC services and other identified potential determinant factors that predispose the newborn to life-threatening (near-miss) conditions especially during this pandemic.
Infusing high-impact practices in undergraduate public health curricula: Models, lessons learned, and administrative considerations from two public universities
Authors: Przybyla Sarahmona M. , Cprek Sarah E. , Kiviniemi Marc T.
Published: September 2022

High-impact practices (HIPs) can improve the rigor, quality, and outcomes of undergraduate education. Several high-impact practices are explicitly woven into the overarching goals, learning objectives, and curricular competencies for undergraduate public health degree programs, while others are natural fits. However, capitalizing on the value of HIPs for public health undergraduates requires a conscious effort in the process of curriculum design, course delivery, and administration of these programs. In this paper, we discuss both conceptual approaches and practical steps involved in the integration of HIPs in curriculum design and implementation. We discuss two exemplars of undergraduate programs that incorporate high-impact practices, illustrating how the same practices can be developed and implemented in different but equally effective ways across programs. We close with practical suggestions for designing or refining an undergraduate program to maximize the inclusion and effectiveness of high-impact practices.
Evaluating industry attempts to influence public health: Applying an ethical framework in understanding commercial determinants of health
Authors: Ndebele Paul , Krisko Perrin , Bari Imran , Paichadze Nino , Hyder Adnan A.
Published: September 2022

Objectives

This paper explores industry influence on public health using a specific case study and applies an established ethical framework based on eleven principles to explore Commercial Determinants of Health (CDoH). It demonstrates an application of these principles to evaluate the ethical integrity of industry strategies and practices and their impacts on public health.

Methods

Using eleven a priori, deductive, ethical principles as codes, this paper conducted an in-depth analysis of 19 e-mail chains and accompanying documents made publicly available through the Freedom of Information Act (FOIA) from U.S. Right to Know (USRTK) sent between Coca-Cola representatives, lobbyists, academics, and the International Life Sciences Institute (ILSI), founded by former Coca-Cola executives.

Results

The three principles violated most frequently amongst the documents were consumer sovereignty (n = 22), evidence-informed actions (n = 21), and transparency (n = 20). Similarly, codes that featured most regularly across documents were transparency (13 out of 19 documents), consumer sovereignty (13 out of 19 documents), evidence-informed actions (9 out of 19 documents), and social justice and equity (9 out of 19 documents). All eleven principles were applied at least four times throughout the documents; however, responsiveness (z = 12), moral responsibility (z = 16), and holism (z = 30) were the least relevant to the data set.

Conclusions

This case study of Coca-Cola demonstrates the usefulness of this ethics framework in reviewing actions of corporate actors in the promotion of products that are harmful to human health. It shows that the industry at times has low ethical integrity in their various strategies and practices to promote their products despite the negative impacts of these products on public health.
How Is Brain Aging Influenced by Where We Live?
Authors: Duchesne Jeanne , Mortamais Marion , Berr Claudine , Benmarhnia Tarik , Letellier Noémie
Published: September 2022

When our brains are healthy, we can memorize, pay attention, reason, move, communicate, make decisions, and complete complex tasks. As some people get older, they can no longer do those things—they suffer from cognitive disorders that could indicate the start of dementia. Many factors might play a role in the development of dementia and some of those factors, like age and education level, have been identified. We know that there are additional, unidentified factors that play a role in the development of dementia, and scientists are discovering that where we live also matters. Our living environments include buildings and other structures, green spaces, and the level of air pollution. In this article, we explain the impact of the living environment on brain health decline during aging.
Effectiveness of minimally invasive surgery using incomplete phalangeal osteotomy for symptomatic curly toe of adults with a trapezoidal phalanx: An observational study
Authors: Ramírez-Andrés Leonor , Nieto-García Eduardo , Nieto-González Elena , López-Ejeda Noemí , Ferrer-Torregrosa Javier
Published: September 2022

Background and aims

Digital deformity in flexion, varismus (external rotation), and adduction with the toe in both supraduction and infraduction are called clinocampodactyly or curly toe. All adult patients with symptoms and a diagnosis of semirigid/rigid curly toes underwent radiological examination to verify the presence of a trapezoidal phalanx. The purpose of this study was to quantitatively determine the degrees of improvement of a dysmetric phalanx after incomplete phalangeal osteotomy using minimally invasive surgery. The points of improvement were determined using the American Orthopedic Foot and Ankle Society (AOFAS) scale score.

Methods

Between May 2021 and June 2022, 30 patients diagnosed with curly toes underwent unicortical osteotomy of the affected phalanx. The convergence angle was measured and the AOFAS scale scores were compared.

Results

A total of 33 toes underwent surgery. The average reduction of the convergence angle was 9°. The average improvement in the AOFAS scale score was 53 points at 6 months and reached almost 90 points (89.9 ± 6.1 points).

Conclusions

Incomplete phalangeal osteotomy performed with minimally invasive surgery of the trapezoidal phalanges of curly toes of adults can improve alignment and AOFAS scale scores.
Femoral head metastases from primary mucinous lung adenocarcinoma with left hip pain: A case report and literature review
Authors: Xue Hang , Zhou Wu , Zhang Zhenhe , Panayi Adriana C. , Xiong Yuan , Yang Shuhua , Mi Bobin , Liu Guohui , Liu Xianzhe
Published: September 2022

Background

Primary mucinous lung adenocarcinoma, a subtype of lung adenocarcinoma, is extremely rare. Currently, as there are no specific diagnostic features, it is easy to delay the diagnosis or even to misdiagnose when atypical symptoms are present.

Case summary

This case details a patient with primary mucinous lung adenocarcinoma and metastasis to the femoral head. The sole symptom was left hip pain and the initial diagnosis was isolated femoral head necrosis.

Conclusions

By presenting this rare case report and the experiences learned from it, we hope to assist clinicians to identify bone metastasis cases with non-typical symptoms in order to make the correct diagnosis as soon as possible.
Major target for UV-induced complete loss of HIV-1 infectivity: A model study of single-stranded RNA enveloped viruses
Authors: Koma Takaaki , Doi Naoya , Suzuki Akihiro , Nagamatsu Kentaro , Yasui Takeshi , Yasutomo Koji , Adachi Akio , Minamikawa Takeo , Nomaguchi Masako
Published: September 2022

Deep ultraviolet light (UV) is useful for the disinfection of microorganisms, including bacteria and viruses. Although genome damage by UV has been widely accepted, the adverse effects of UV on the activity and/or function of viral proteins including the envelope components are poorly documented. Worthy of note, the observed unfavorable UV-effects for viruses are only insufficiently analyzed in association with the reduction in viral infectivity. In this study, we aimed to clarify which component of virions affected by UV significantly correlates with the loss of viral infectivity using HIV-1 as a model for single-stranded RNA enveloped viruses. Using our UV irradiation apparatus at three wavelengths (265, 280, and 300 nm), we first quantitatively determined the UV power density and irradiation period of each wavelength required for a reduction in infectivity. A heat-treated sample as a control drastically reduced the virion-associated reverse transcriptase (RT) activity and Gag-p24 level. The UV-irradiated samples at the three wavelengths, completely lacking viral infectivity, showed p24 levels similar to those without irradiation. While the virion-associated RT activity was gradually decreased in a wavelength and power density dependent manner, this reduction did not explain the loss of viral infectivity by UV. Remarkably, virological assays revealed that the entry efficiency of the UV-irradiated virus samples at the three wavelengths is comparable to those without irradiation. Importantly, this result shows that, even the virions exposed to UV of various wavelengths at the lethal level, still maintain the function of their envelope composed of a host lipid bilayer and viral proteins. In sharp contrast, UV-induced genome damage shown by semiquantitative RT-PCR correlated well with the reduction in viral infectivity, indicating that it is a major determinant for virus inactivation by UV. The degree of damage was found to be distinct among the regions analyzed. This was probably due to the different nucleotide sequences in those genomic regions amplified by PCR. Our data clearly demonstrate a principal mechanism for viral inactivation by UV and provide information contributing to the improvement of UV-based disinfection technology for microorganisms.
The Mechanical and Antibacterial Properties of Boron Nitride/Silver Nanocomposite Enhanced Polymethyl Methacrylate Resin for Application in Oral Denture Bases
Authors: Li Miao , Wang Sifan , Li Ruizhi , Wang Yuting , Fan Xinyue , Gong Wanru , Ma Yu
Published: September 2022

The introduction of nanomaterials into polymethyl methacrylate (PMMA) resin has been effective for mechanically reinforcing PMMA for application in oral denture bases. However, these methods cannot simultaneously improve the mechanical and antibacterial properties, which limits widespread clinical application. Here, we self-assembled binary nanocomposites of boron nitride nanosheets (h-BNNs) and silver nanoparticles (AgNPs) as nanofillers and incorporated the nanofillers into PMMA. The aim of this study was to achieve antibacterial effects while significantly improving the mechanical properties of PMMA and provide a theoretical basis for further clinical application. We employed scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy (Raman), Ultraviolet visible spectrum (UV) and atomic force microscopy (AFM) to investigate the microscopic morphology and composition of PMMA containing nanocomposites with different mass fraction. In addition, the content of the h-BNNs/AgNPs was 1 wt%, and the compressive strength and flexural strength of pure PMMA were improved by 53.5% and 56.7%, respectively. When the concentration of the nanocomposite in the PMMA resin was 1.4 wt%, the antibacterial rate was 92.1%. Overall, synergistically reinforcing PMMA composite resin with a multi-dimensional nanocomposite structure provided a new perspective for expanding not only the application of resins in clinical settings but also the research and development of new composite resins.
Electrochemical Sensing of Favipiravir with an Innovative Water-Dispersible Molecularly Imprinted Polymer Based on the Bimetallic Metal-Organic Framework: Comparison of Morphological Effects
Authors: Erk Nevin , Mehmandoust Mohammad , Soylak Mustafa
Published: September 2022

Molecularly imprinted polymers (MIPs) are widely used as modifiers in electrochemical sensors due to their high sensitivity and promise of inexpensive mass manufacturing. Here, we propose and demonstrate a novel MIP-sensor that can measure the electrochemical activity of favipiravir (FAV) as an antiviral drug, thereby enabling quantification of the concentration of FAV in biological and river water samples and in real-time. MOF nanoparticles’ application with various shapes to determine FAV at nanomolar concentrations was described. Two different MOF nanoparticle shapes (dodecahedron and sheets) were systematically compared to evaluate the electrochemical performance of FAV. After carefully examining two different morphologies of MIP-Co-Ni@MOF, the nanosheet form showed a higher performance and efficiency than the nanododecahedron. When MIP-Co/Ni@MOF-based and NIP-Co/Ni@MOF electrodes (nanosheets) were used instead, the minimum target concentrations detected were 7.5 × 10−11 (MIP-Co-Ni@MOF) and 8.17 × 10−9 M (NIP-Co-Ni@MOF), respectively. This is a significant improvement (>102), which is assigned to the large active surface area and high fraction of surface atoms, increasing the amount of greater analyte adsorption during binding. Therefore, water-dispersible MIP-Co-Ni@MOF nanosheets were successfully applied for trace-level determination of FAV in biological and water samples. Our findings seem to provide useful guidance in the molecularly imprinted polymer design of MOF-based materials to help establish quantitative rules in designing MOF-based sensors for point of care (POC) systems.
Fluorescent RET-Based Chemosensor Bearing 1,8-Naphthalimide and Styrylpyridine Chromophores for Ratiometric Detection of Hg2+ and Its Bio-Application
Authors: Panchenko Pavel , Efremenko Anastasija , Polyakova Anna , Feofanov Alexey , Ustimova Maria , Fedorov Yuri , Fedorova Olga
Published: September 2022

Dyad compound NI-SP bearing 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units, in which the N-phenylazadithia-15-crown-5 ether receptor is linked with the energy donor naphthalimide chromophore, has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In an aqueous solution, NI-SP selectively responds to the presence of Hg2+ via the enhancement in the emission intensity of NI due to the inhibition of the photoinduced electron transfer from the receptor to the NI fragment. At the same time, the long wavelength fluorescence band of SP, arising as a result of resonance energy transfer from the excited NI unit, appears to be virtually unchanged upon Hg2+ binding. This allows self-calibration of the optical response. The observed spectral behavior is consistent with the formation of the (NI-SP)·Hg2+ complex (dissociation constant 0.13 ± 0.04 µM). Bio-imaging studies showed that the ratio of fluorescence intensity in the 440–510 nm spectral region to that in the 590–650 nm region increases from 1.1 to 2.8 when cells are exposed to an increasing concentration of mercury (II) ions, thus enabling the detection of intracellular Hg2+ ions and their quantitative analysis in the 0.04–1.65 μM concentration range.
Does Executive Function Training Impact on Communication? A Randomized Controlled tDCS Study on Post-Stroke Aphasia
Authors: Pisano Francesca , Manfredini Alessio , Castellano Andrea , Caltagirone Carlo , Marangolo Paola
Published: September 2022

New approaches in aphasia rehabilitation have recently identified the crucial role of executive functions (EFs) in language recovery, especially for people with severe aphasia (PWSA). Indeed, EFs include high-order cognitive abilities such as planning and problem solving, which enable humans to adapt to novel situations and are essential for everyday functional communication. In a randomized double-blind crossover design, twenty chronic Italian PWSA underwent ten days of transcranial direct current stimulation (tDCS) (20 min, 2 mA) over the right dorsolateral prefrontal cortex (DLPFC). Two conditions were considered, i.e., anodal and sham, while performing four types of cognitive training (alertness, selective attention, visuo-spatial working memory, and planning), all of which were related to executive functions. After anodal tDCS, a greater improvement in selective attention, visuospatial working memory and planning abilities was found compared to the sham condition; this improvement persisted one month after the intervention. Importantly, a significant improvement was also observed in functional communication, as measured through the Communication Activities of Daily Living Scale, in noun and verb naming, in auditory and written language comprehension tasks and in executive function abilities. This evidence emphasizes, for the first time, that tDCS over the right DLPFC combined with executive training enhances functional communication in severe aphasia.
Review of the Strengthening Methods and Mechanical Properties of Recycled Aggregate Concrete (RAC)
Authors: Liu Chuanqi , Wang Yanjun , Gao Xuyang , Zhang Guanglong , Liu Heng , Ma Chong , Sun Jilin , Lai Jinxing
Published: September 2022

Replacing natural aggregate (NA) with recycled aggregate (RA) has contributed to the trend of sustainable development in civil construction. With this background, improvements in the mechanical properties of recycled aggregate concrete (RAC) and the scientific design of the mixture ratio are attracting more concern in recent years. This paper is a review of the recent research, including the following aspects: the mixture design of RAC; the improved mechanical properties of recycled concrete with steel fibers; and the performance of the main components. In addition, the primary composition materials, properties, and calculation methods of the mixture ratio of RAC are summarized. The mechanical properties, durability and microscopic analysis of RAC are also discussed. The accurate calculation of mixture proportion can significantly facilitate the work of preparing a test mix of RAC. Through the mixture-ratio optimization and physical and chemical strengthening of RA, the mechanical properties of RAC can be improved to promote the wider application of this eco-friendly material.
Four Methods to Distinguish between Fractal Dimensions in Time Series through Recurrence Quantification Analysis
Authors: Tomashin Alon , Leonardi Giuseppe , Wallot Sebastian
Published: September 2022

Fractal properties in time series of human behavior and physiology are quite ubiquitous, and several methods to capture such properties have been proposed in the past decades. Fractal properties are marked by similarities in statistical characteristics over time and space, and it has been suggested that such properties can be well-captured through recurrence quantification analysis. However, no methods to capture fractal fluctuations by means of recurrence-based methods have been developed yet. The present paper takes this suggestion as a point of departure to propose and test several approaches to quantifying fractal fluctuations in synthetic and empirical time-series data using recurrence-based analysis. We show that such measures can be extracted based on recurrence plots, and contrast the different approaches in terms of their accuracy and range of applicability.
Experimental Study of the Influence of Different Load Changes in Inlet Gas and Solvent Flow Rate on CO2 Absorption in a Sieve Tray Column
Authors: Almoslh Adel , Aghel Babak , Alobaid Falah , Heinze Christian , Epple Bernd
Published: September 2022

An experimental study was conducted in a sieve tray column. This study used a simulated flue gas consisting of 30% CO2 and 70%. A 10% mass fraction of methyl diethanolamine (MDEA) aqueous solution was used as a solvent. Three ramp-up tests were performed to investigate the effect of different load changes in inlet gas and solvent flow rate on CO2 absorption. The rate of change in gas flow rate was 0.1 Nm3/h/s, and the rate of change in MDEA aqueous solution was about 0.7 NL/h/s. It was found that different load changes in inlet gas and solvent flow rate significantly affect the CO2 volume fraction at the outlet during the transient state. The CO2 volume fraction reaches a peak value during the transient state. The effect of different load changes in inlet gas and solvent flow rate on the hydrodynamic properties of the sieve tray were also investigated. The authors studied the correlation between the performance of the absorber column for CO2 capture during the transient state and the hydrodynamic properties of the sieve tray. In addition, this paper presents an experimental investigation of the bubble-liquid interaction as a contributor to entropy generation on a sieve tray in the absorption column used for CO2 absorption during the transient state of different load changes.