bluuebunny/crossref_metadata_2025 huggingface dataset:
BEHAVIORAL APPROACH TO DECISION-MAKING IN THE FINANCIAL MARKET
Authors:   V. N. Karazin Kharkiv National University, Ivashchenko Maryna
Published: January 2024

An analysis of the behavioral aspects of decision-making in the financial market was carried out and the main theoretical approaches to explaining behavioral differences in the decisions of financial agents were considered. The role of cognitive biases, heuristics, behavioral illusions, and psychological factors in the formation of decisions of investors and financial market participants is summarized. Behavioral effects in making financial decisions are systematized according to their common characteristics. The importance of psychological factors in the formation of trends and price dynamics on the market has been proven. Significant aspects that determine the behavioral approach to financial transactions and affect the effectiveness of investment and portfolio management are revealed. The dependence between the features of a person’s mental activity and the ability to make logical rational decisions has been revealed. The role of cognitive biases in protecting an individual from excessive amounts of information and its simplification is substantiated.
Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci
Authors: Loubna Yacoubi  , Mouad Harandou  , Amina Himri  , Zainab Kajeiou  , Sabah Mokhtari  , Issam Mokhtari  , Nisma Douzi  , El houcine Sebbar  , Mohammed Choukri
Published: January 2024

Introduction: The aim of our study is to evaluate the haptoglobin assay on the Alinity ci automated system. This evaluation is part of an overall approach to verifying the methods used in the central laboratory of the Mohammed VI University Hospital of Oujda , with a view to compiling an accreditation file in accordance with the requirements of standard NF ISO 15189. Materials and methods: The working methodology adapted by our study is based on the recommendations of the protocol of the COFRAC accreditation technical guide SH GTA 04. Verification involved assessing the repeatability and reproducibility of Alinity ci . Results: The results obtained for the various haptoglobin assay verification criteria show satisfactory repeatability for all three levels, with CV1=1.10%, CV2=0.78% and CV3=1.07% respectively. intra-laboratory reproducibility was satisfactory for all three levels, with CV1=1.79%, CV2= 4.2% and CV3=1.62% respectively. Discussion: Verification of an analytical method is an essential step in guaranteeing that the result obtained is as close as possible to the reference value of a sample. Comparing our results with the CV adopted by the SFBC, we can see that the results are in line with and below the tolerated limits. Conclusion: We can therefore conclude that the Abbott Alinity ci system meets the requirements set by scientific societies for the determination of haptoglobin.
A pilot study on determination of dietary intake of geriatric epileptic patients
Authors: Preethi Rathinam  , Lally Hanna Luke  , Deepa C Philip
Published: January 2024

Epilepsy, a complex neurological condition, stems from multifaceted factors, including genetic predispositions and various triggers. The elderly, a rapidly growing demographic globally, encounter an increased risk of seizures. In India, a significant population lives with epilepsy, notably more prevalent in rural areas. Moreover, malnutrition and epilepsy exhibit a complex relationship, potentially influencing each other's onset and progression. Geriatric individuals with epilepsy often face sedentary lifestyles and potential weight-related concerns due to medication. This pilot study aimed to assess the nutrient profile of geriatric patients with epilepsy. A quantitative research approach was adopted; studying 30 individuals aged 60 to 80 years at a private neurology hospital in Tamil Nadu. Data was collected through interviews and dietary assessments, including the 24-hour recall and food frequency methods. Statistical analysis using SPSS provided insights into nutrient intake among the participants. Results indicated that while certain nutrients met recommended levels, fat and carbohydrate intake exceeded the prescribed amounts. Additionally, dietary patterns varied among different types of seizures. Despite differences, no statistical significance was found. Food frequency analysis revealed varied consumption patterns, notably lower intake of fruits and vegetables among the epilepsy geriatric patients. The study emphasizes the importance of understanding the nutritional needs of individuals with epilepsy, particularly in the geriatric population, to formulate personalized dietary interventions for improved seizure management and overall health.
Nutritional status of pregnant women and its impact on child stunting incidence in Indonesia: Literature review
Authors: Gayatri Ayu Prameswari
Published: January 2024

The aim of this research is to determine the relationship between the nutritional status of pregnant women and the incidence of stunting in children in Indonesia based on a review of several related studies. The research method used is a literature review by searching for research articles related to the topic of stunting and nutrition of pregnant women in Indonesia in the last 10 years from online databases. Relevant articles were collected and analyzed systematically. Several studies show that pregnant women with poor nutritional intake and status are at risk of giving birth to babies with stunting and low birth weight. Other factors such as diet, personal hygiene and infections also influence the nutritional status of pregnant women. Poor nutritional status of pregnant women contributes significantly to the risk of stunting in children. Optimizing the nutrition of pregnant women through supplementation, education and increasing access to nutritious food is important as an effort to prevent stunting from an early age.
The effect of lemon aromatherapy inhalation on reducing the degree of emesis gravidarum in first trimester pregnant women at TPMB Puji Astuti Surabaya
Authors: Hanny Nur Azizah  , Euvanggelia Dwilda Ferdinandus  , Bambang Purwanto  , Farida Fitriana
Published: January 2024

One of the discomforts that occurs in pregnant women in the first trimester is nausea, vomiting (emesis gravidarum). Emesis gravidarum causes discomfort due to feelings of dizziness, flatulence, and a feeling of weakness in the body. Emesis gravidarum can be treated with nutritional therapy, nonpharmacological therapy and pharmacological therapy. Lemon aromatherapy is a non-pharmacological therapy that can be used by pregnant women to reduce emesis gravidarum. The aim of this research is to determine the effect of lemon aromatherapy inhalation on reducing the degree of emesis gravidarum in pregnant women in the 1st trimester. Method: This research is true-experimental research with a two group pretest-posttest design. The instrument used was the Nausea Vomiting Index Nausea Vomitting and Retching (INVR) questionnaire. Data analysis used the Wilcoxon test to determine the effect of inhalation from the two groups. Results: In the intervention group, after being given treatment, they got a mean score of 1.94 with a standard deviation of 0.659. Based on the Wilcoxon test, a p-value of 0.000 was obtained, which means it is smaller than the significant value, namely <0.05. So it can be concluded that there was an effect in the intervention group before and after giving lemon aromatherapy, compared with the control group, the emesis gravidarum score before was found to be a mean of 2.82 with a standard deviation of 0.728. and after getting a value with a mean of 2.76 with a standard deviation of 0.664, based on the Wilcoxon test, a p-value of 0.317 was obtained, which means it is greater than the significant value, namely >0.05. So it can be concluded that there was no effect on the control group before and after not being given treatment at TPMB Puji Astuti Surabaya. Conclusion: There is an effect of giving lemon aromatherapy inhalation on reducing the degree of emesis gravidarum in pregnant women in the 1st trimester.
History of Glucopuncture
Authors: Kersschot J
Published: January 2024

Glucopuncture is a treatment option originating from Europe (Austria) and Asia (South Korea). It is a medical technique which uses regional injections with 5% sugar water such as D5W (or G5W) into dermis, fascia, joints, muscles and ligaments. Pain modulation is mainly achieved by intradermal, fascial and US-guided perineural injections. Biotensegrity regulation is mainly achieved by injecting into the superficial layer of regional fascia. Functional improvement is achieved by giving injections into muscles. Such intramuscular injections can support and accelerate tissue repair, which is an interesting tool when treating sports injuries in professional athletes. Especially injections into superficial fascia are becoming more popular recently because of easy application and interesting clinical outcome. Over the last decades, clinicians worldwide came to see that regional D5W injections are safe, inexpensive and efficient tools to manage musculoskeletal pain and sports injuries. Both patient-guided as well as screen-guided glucopuncture gain popularity worldwide. Clinical research so far was mainly focused on treatment of carpal tunnel syndrome with D5W.
Optimasi Kodon dan Konstruksi Plasmid Rekombinan Protein RBD SARS-CoV-2 pada E. coli BL21(DE3)
Authors: Purnamasari Elly Widyarni Eka , Dini Wahyu Kartika Sari  , Tenri Ashari Wanahari  , Khairun Nissa  , Kusumawati Asmarani
Published: January 2024

Coronavirus Disease 2019 (COVID-19), yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), telah menyebabkan lebih dari 7 juta kematian di seluruh dunia. Strategi komprehensif diperlukan untuk mengatasi pandemi ini, terutama vaksinasi untuk mengurangi infeksi lebih lanjut. Pengembangan vaksin bergantung pada pemanfaatan protein imunogenik dari virus SARS-CoV-2, khususnya pada Receptor-binding Domain (RBD) yang terletak dalam Subunit 1 Spike Glycoprotein. Protein ini berperan penting dalam interaksi virus dengan sel inang melalui reseptor ACE2 untuk menginfeksi inang bersama dengan Subunit 2 untuk memediasi fusi pada membran sel. Meskipun beberapa vaksin telah tersedia, kebutuhan terhadap alternatif vaksin yang lebih terjangkau dan mudah diakses masih terus dikembangkan, terutama untuk negara-negara berkembang dan berpenghasilan rendah. Salah satu alternatif yang dapat dikembangkan dapat berupa vaksin berbasis protein rekombinan. Dalam penelitian ini, urutan gen RBD dioptimasi untuk digunakan pada konstruksi plasmid rekombinan pada ekspresi protein heterolog dalam sistem ekspresi prokaryot yaitu Escherichia coli BL21(DE3). Kodon yang telah dioptimasi kemudian disisipkan ke dalam vektor plasmid pET-15b dan dikonstruksikan menjadi plasmid rekombinan untuk transformasi ke dalam sel inang dengan metode heat-shock. Penelitian ini dievaluasi dan divalidasi melalui PCR koloni, isolasi plasmid, dan sekuensing. Hasil pada penelitian ini dapat menjadi dasar bagi penelitian lanjutan pada ekspresi dan purifikasi protein RBD SARS-CoV-2 pada E. coli BL21(DE3) sebagai kandidat vaksin protein subunit rekombinan atau uji diagnostik pada penelitian terkait COVID-19.
Efektivitas Terapi Relaksasi Otot Progresif dan Terapi Musik Suara Alam Terhadap Tingkat Stres Mahasiswa Semester Akhir
Authors: Cahyani Ardhia Redina , Tasalim Rian
Published: January 2024

Mahasiswa tingkat akhir memiliki kepentingan akademik seperti tugas yang banyak dan memiliki banyak tuntutan dari dirinya sendiri maupun dari tuntutan eksternal yang menyebabkan mahasiswa lebih rentan mengalami stres. Stres yang berkepanjangan dan tidak diatasi dapat menimbulkan gangguan fisik dan psikologis. Stres dapat ditangani secara farmakologi dan non farmakologi. Ada beberapa cara non farmakologi yang dapat dilakukan untuk menurunkan tingkat stres yaitu terapi relaksasi otot progesif dan terapi musik.  Penelitian ini secara umum bertujuan untuk mengetahui pengaruh terapi relaksasi otot progesif dan terapi musik suara alam terhadap tingkat stres mahasiswa semester akhir. Penelitian ini menggunakan pendekatan kuantitatif dengan menggunakan desain pre-experimental. Jumlah sampel sebanyak 8 orang yang diambil dengan teknik accidental sampling, berdasarkan kriteria inklusi dan eksklusi. Teknik pengambilan data menggunakan kuesioner PSS. Analisis yang digunakan uji Wilcoxon. Hasil uji bivariat didapatkan adanya pengaruh teknik relaksasi otot progresif dan terapi musik suara alam terhadap tingkat stres mahasiswa semester akhir dengan nilai significane 0,012. Bagi bidang kesehatan khususnya keperawatan diharapkan dapat menerapkan teknik relaksasi otot progresif dan terapi musik suara alam untuk menurunkan tingkat stres.
CONTRIBUTION OF ANZAR SHAH KASHMIRI TO HADITH AND ISLAMIC SCIENCES
Authors: Ajmal Mohammad , Al Rasyid Harun
Published: January 2024

Penelitian ini, menyoroti kontribusi Anzar Shah Kashmir terhadap hadis dan ilmu-ilmu keislaman. Penelitian ini berkonsentrasi pada salah satu tokoh terkemuka Darul Uloom Deoband yang mengabdikan seluruh hidupnya untuk pelayanan Al-Qur'an dan Hadits, dan merupakan seorang guru Hadits dan Tafsir serta sarjana ilmu-ilmu dari dua subjek paling otentik dalam studi dan penelitian Islam ini. Untuk menulis penelitian ini, saya membaca dan meneliti banyak artikel dalam bahasa Arab dan Inggris dan Urdu, dari berbagai penulis yang berbeda tentang Syekh Anzar Shah Kashmir untuk menyelesaikan makalah ini. Syekh Anzar Shah Kashmir adalah salah satu cendekiawan besar ilmu-ilmu Islam di India, yang melalui pengetahuannya yang luas tentang Hadis dan ilmu-ilmu Al-Quran, mentransmisikan informasi tersebut kepada para murid dan mahasiswanya secara khusus, sementara melalui pidato dan pidato-pidatonya di depan umum, informasi tersebut ditransfer ke masyarakat umum secara luas.

 

This research paper, has tried to highlight the contribution of Anzar Shah Kashmiri to hadith and Islamic sciences. The research concentrates on one of the prominent figures of Darul Uloom Deoband who devoted his entire life to the service of the Qur'an and Hadith, and was a teacher of Hadith and Tafsir as well as a scholar of the sciences of these two most authentic subjects in Islamic studies and research. To write this research, I read and researched many articles in Arabic and English and Urdu, from different authors about Sheikh Anzar Shah Kashmiri to complete this paper. Sheikh Anzar Shah Kashmiri was one of the great scholars of Islamic sciences in India, who through his vast knowledge of Hadith and Quranic sciences, transmitted the information to his disciples and students in particular, while through his public speeches and addresses, the information was transferred to the general public in general.

Keyword : Anzar Shah Kashmiri, Hadith, Tafsir, Darul Uloom Deoband, Islamic scholars in India
AKSELERASI EKONOMI BERKELANJUTAN MELALUI PEMBERDAYAAN MASYARAKAT DESA PADA KEGIATAN DAUR ULANG SAMPAH PLASTIK DI DESA KALANGKANGAN KECAMATAN GALANG KABUPATEN TOLITOLI
Authors: Yanto Edi
Published: January 2024

Pengabdian kepada masyarakat yang dilakukan merupakan seminar pemberdayaan masyarakat desa pada kegiatan daur ulang sampah plastik di Desa Kalangkangan Kecamatan Galang Kabupaten Tolitoli. Kegiatan ini bekerja sama dengan Bank Sampah Tolitoli Mandiri dan bertujuan mengakselerasi ekonomi berkelanjutan melalui pemanfaatan dan pemilahan limbah plastik untuk dikonversi menjadi rupiah dan bernilai ekonomi bagi peningkatan ekonomi dan kesejahteraan keluarga. Kegiatan yang diikuti oleh 18 orang peserta merupakan kalangan ibu rumah tangga sehingga berkontribusi nyata pada penambahan pendapatan keluarga. Hasilnya, selama 30 hari rata-rata nilai konversi yang dihasilkan adalah Rp 219,861,- (87,94 Kg) dengan peningkatan level pengetahuan dan pemahaman terhadap materi terkait akselerasi ekonomi berkelanjutan melalui pamanfaatan limbah plastik untuk bahan daur ulang. Guna memfasilitasi layanan BSTM, dibuat grup WA “Nasabah BSTM” sehingga mempermudah dan menjamin layanan informasi dan operasional terhadap nasabah.
PENERAPAN KOMPOR BIOMASSA TIPE CONTINUE BAHAN BAKAR PADA UMKM DODOL MATUARI
Authors: Djafar Romi
Published: January 2024

Sampai  saat  ini  jumlah  pengguna  tungku  kayu  bakar  tradisional  masih  tetap  tinggi untuk keperluan sehari-hari disektor rumah tangga  dan UMKM.  Salah satunya terdapat pada MITRA pengrajin  kuliner  khas  Gorontalo  seperti  dodol  di  desa  Reksonegoro. Oleh itu Penerapan kompor biomassa ini sangat efektif digunakan mengingat ketersediaan bakar yang melimpah. Berdasarkan observasi lapangan kendala utama yang dihadapi UMKM dodol Matuari adalah Tungku perapian yang masih sederhana seperti menggunakan tiga buah batu memasak dodol. Keadaan ini dinilai  kurang efektif dalam meningkatkan kapasitas produksi.

 Kegiatan Pengabdian ini bertujuan untuk memberikan sentuhan teknologi tepat guna melalui penerapan kompor biomasa atau Tungku Sehat Hemat (TSHE). Disamping itu pada kegiatan pengabdian ini juga dilakukan pendampingan cara mengolah dodol cara pengolahan pangan olahan yang baik (CPPOB) dan Sanitation Standard  Operating  Procedures  (SSOP). 

Berdasarkan kegiatan Pengabdian diperoleh beberapa hasil yaitu Fabrikasi Kompor biomasa yang memiliki dimensi 49 cm dan bobot sekitar 50 kg dengan hasil Uji coba perpaian bahan baku dodol sebanyak 4 Kg dimana efisiensi waktu lebih singkat sebesar 28.5% dibandingan dengan metode konvensional. Sedangkan kebutuhan bahan bakar dalam pengujian lebih hemat sebesar 35% dari seperti biasanya. Selain itu diperoleh desain kemasan dodol yang informatif memiliki ukuran 10 x 15 x 7 cm (PXLXT) untuk kapasitas 5 pcs dodol.

Kedepan kegiatan pengabdian ini khususnya penerapan teknologi kompor TSHE perlu dikembangkan pada UMKM yang lain agar diperoleh Fabrikasi TSHE  yang fix Desain agar dapat di produksi secara masal.
SOSIALISASI STRATEGI PENGEMBANGAN POTENSI BISNIS PADA UMKM DI KECAMATAN PURWOKERTO SELATAN
Authors: Putri Puspita Lianti
Published: January 2024

Usaha Mikro, Kecil dan Menengah (UMKM) merupakan salah satu kegiatan usaha yang berkontribusi besar dalam perekonomian nasional. Persaingan yang semakin ketat ini didukung dengan kemajuan teknologi yang semakin berkembang, sehingga mengharuskan UMKM untuk mampu beradaptasi dengan cepat oleh perubahan ini. Strategi bisnis membantu perusahaan untuk mengantisipasi masalah dan menyiasati trend bisnis yang terus berubah. Sehingga rintangan yang menghadang, maupun tujuan yang ditetapkan dapat terwujud. Seperti strategi untuk menarik lebih banyak sales lead, meningkatkan akuisisi pelanggan, meningkatkan kepuasan pelanggan, meningkatkan kualitas produk dan penjualan, atau menghasilkan revenue bisnis dan profitabilitas sebanyak-banyaknya. Strategi bisnis penting bagi pelaku UMKM karena strategi yang baik dapat membantu UMKM mengidentifikasi langkah-langkah kunci yang akan diambil guna mencapai tujuan bisnis. Sosialisasi ini bertujuan untuk memberikan pemahaman pelaku UMKM di Kecamatan Purwokerto Selatan untuk meningkatkan keunggulan kompetitif.
Laparoscopic Correction of Cesarean Scar Defects by Temporary Bilateral Uterine Artery Occlusion
Authors: Tang Fei , Ji Limei , Jin Lanying , Shao Mingjun , Hu Min
Published: January 2024

Background: The aim of our study was to evaluate the clinical efficacy of temporary bilateral uterine artery blockage for cesarean scar defects when combined with laparoscopy and hysteroscopy. Methods: We evaluated 126 patients who had one or more cesarean deliveries with abnormal uterine bleeding (12–20 days). All eligible women were informed of the potential complications, benefits, and alternatives, for each approach before they were assigned into one of two treatment groups. Group A received temporary bilateral uterine artery occlusion and vasopressin injection while Group B received vasopressin injection only. Results: Mean blood loss was 54.70 ± 13.01 mL and 190.82 ± 15.72 mL in Groups A and B (p < 0.001). By the final evaluation, the mean duration of menstruation had reduced to 6.92 ± 2.16 and 7.16 ± 2.25 days in Group A and Group B; these values were significantly different than the pre-operative values (p < 0.001 respectively). The mean thinnest residual myometrium was 5.39 ± 0.77 and 5.28 ± 1.25 mm in Group A and Group B, respectively. These values were thicker than pre-operative values (p < 0.001 respectively). The efficacy of anatomic correction was 96.88% (62/64) and 96.77% (60/62) (p > 0.999) in Groups A and B, respectively. Overall, 58 of the 64 (90.63%) patients in Group A and 57 of the 62 (91.94%) patients in Group B reported an improved menstrual cycle following surgery (p = 0.794). Conclusions: The combination of laparoscopy, hysteroscopy, temporary bilateral uterine artery occlusion, and the injection of vasopressin, offers an effective measure to reduce blood loss effectively.
Early Prediction of Placenta Accreta Spectrum by Different Modalities: An Evidenced-based Analysis
Authors: Elbasuiny Heba Rady , Dawood Ayman Shehata , Raslan Mohamed Atia
Published: January 2024

Objective: Early prediction of placenta accreta is crucial for proper decision making, proper preoperative preparation and proper planning for the best management. To review published data and extract strong evidence about early prediction of placenta accreta spectrum (PAS). Mechanism: Published data were extracted from trusted search engines and literature databases, such as Google Scholar, Cochrane library, Clinical Key, PubMed, Researchgate, and Medline, in the period from 1 January 2000 to 31 August 2023. Revision of collected data was conducted by the 3 authors and final results were gathered. Findings in Brief: Many articles were found addressing prediction of PAS but the vast majority of these articles were in the late second and third trimesters. Prediction of PAS in early pregnancy was addressed by few articles which were analyzed in this research. In this study, evidence-based analysis found that PAS could be predicted by many modalities. Conclusions: Early prediction of PAS allows good preparation of both patient and obstetrician to conduct the best management with minimal complications. PAS could be predicted early by either clinical risk factor, imaging in early pregnancy or by biomarkers.
Bioinformatics Analysis Identifies PLA2G7 as a Key Antigen-Presenting Prognostic Related Gene Promoting Hepatocellular Carcinoma through the STAT1/PD-L1 Axis
Authors: Guo Sihang , Yang Qinhe
Published: January 2024

Background: Antigen presentation may be an important factor contributing to immune evasion in cancer. This study investigated antigen-presenting prognostic related genes (APPGs) and their potential mechanisms in hepatocellular carcinoma (HCC). Methods: We constructed a score built upon the core APPGs (APP.Score) through nonnegative matrix factorization (NMF) clustering, weighted gene co-expression network analysis (WGCNA), random forest (RF), and least absolute shrinkage and selection operator (LASSO) methods. We also compared the clinical and molecular characteristics of different APP.Score. Furthermore, in vitro experiments were conducted to validate the expression of core APPGs and investigate the effects of phospholipase A2, group 7 (PLA2G7) knockdown on HCC cell development and programmed death-ligand 1 (PD-L1) expression. Results: APP.Score was positively correlated with immune cell infiltration and levels of immune checkpoint inhibitor-related genes, and negatively correlated with overall survival (OS). The area under the curve values were 0.734, 0.747, and 0.679 for survival periods of 1, 2, and 3 years, respectively, indicating that APP.Score could be an independent prognostic factor for patients with HCC. OS of the high expression group of these genes, including PLA2G7, musculin, heat shock protein family A, secreted phosphoprotein 1, and neutrophil cytosolic factor 2 (NCF2) was lower than that of their low expression group. Moreover, the upregulation of key components of APPGs, except NCF2, was observed in HCC. The inhibition of PLA2G7 suppressed HCC progression and reduced PD-L1 and phosphorylated signal transducer and activator of transcription 1 (p-STAT1)/STAT1 levels in HepG2 and Huh-7 cells. Remarkably, the decrease in PD-L1 expression caused by PLA2G7 silencing was reversed upon treatment with a STAT1 activator. Conclusion: The results of this study show that APP.Score could be an independent prognostic factor for patients with HCC, and that PLA2G7 silencing inhibits cancer cell development and PD-L1 expression. We provide a new perspective and potential target for immune research on antigen presentation in HCC.
Sanguinarine Triggers Apoptosis in Cutaneous Squamous Cell Carcinoma Cells through Reactive Oxygen Species-Dependent c-Jun N-Terminal Kinase Signaling Pathway
Authors: Patil Kalyani , Khan Abdul Q , Ahmad Fareed , Kuttikrishnan Shilpa , Anver Rasheeda , Mateo Jericha M. , Ahmad Aamir , Bhat Ajaz A. , Buddenkotte Joerg , Steinhoff Martin , Uddin Shahab
Published: January 2024

Background: The benzophenanthridine Sanguinarine (Sng) is one of the most abundant root alkaloids with a long history of investigation and pharmaceutical applications. The cytotoxicity of Sng against various tumor cells is well-established; however, its antiproliferative and apoptotic potential against the cutaneous squamous cell carcinoma (cSCC) cells remains unknown. In the present study, we investigated the anti-cancer potential of Sng against cSCC cells and elucidated the underlying mechanisms relevant to the drug action. Methods: The inhibitory effect of Sng on cSCC cells was evaluated by analyzing cell viability, colony-forming ability and multi-caspase activity. Apoptosis was quantified through Annexin-V/Propidium iodide flow cytometric assay and antagonized by pan-caspase inhibitor z-VAD-FMK. Mitochondrial membrane potential (ΔΨm) dysfunction was analyzed by JC-1 staining, whereas reactive oxygen species (ROS) generation was confirmed by pretreatment with N-acetylcysteine (NAC) and fluorogenic probe-based flow cytometric detection. The expression of cell cycle regulatory proteins, apoptotic proteins and MAPK signaling molecules was determined by Western blotting. Involvement of JNK, p38-MAPK and MEK/ERK in ROS-mediated apoptosis was investigated by pretreatment with SP600125 (JNK inhibitor), SB203580 (p38 inhibitor) and U0126 (ERK1/2 inhibitor), respectively. The stemness-targeting potential of Sng was assessed in tumor cell-derived spheroids. Results: Treatment with Sng decreased cell viability and colony formation in primary (A431) and metastatic (A388) cSCC cells in a time- and dose-dependent manner. Sng significantly inhibited cell proliferation by inducing sub-G0/G1 cell-cycle arrest and apoptosis in cSCC cells. Sng evoked ROS generation, intracellular glutathione (GSH) depletion, ΔΨm depolarization and the activation of JNK pathway as well as that of caspase-3, -8, -9, and PARP. Antioxidant NAC inhibited ROS production, replenished GSH levels, and abolished apoptosis induced by Sng by downregulating JNK. Pretreatment with z-VAD-FMK inhibited Sng-mediated apoptosis. The pharmacological inhibition of JNK by SP600125 mitigated Sng-induced apoptosis in metastatic cSCC cells. Finally, Sng ablated the stemness of metastatic cSCC cell-derived spheroids. Conclusion: Our results indicate that Sng exerts a potent cytotoxic effect against cSCC cells that is underscored by a mechanism involving multiple levels of cooperation, including cell-cycle sub-G0/G1 arrest and apoptosis induction through ROS-dependent activation of the JNK signaling pathway. This study provides insight into the potential therapeutic application of Sng targeting cSCC.
Novel Targeted Therapies for Rheumatoid Arthritis Based on Intracellular Signalling and Immunometabolic Changes: A Narrative Review
Authors: Rahmati Marveh , Kwesiga Maria Paula , Lou Jiachen , Tan Ai Lyn , McDermott Michael F
Published: January 2024

Rheumatoid arthritis (RA) is a relatively common systemic autoimmune disease with an estimated prevalence of approximately 1% worldwide. Patients present predominantly with symmetrical small joint inflammatory arthritis, which involves dysregulated immune responses, leading to bone and cartilage deformities due to extensive erosive damage. The introduction of biological based therapies for the management of this life-altering condition, over the past three decades, has led to marked improvements in patients’ quality of life. A wide range of both innate and adaptive immune cells are involved in the pathogenesis of RA, with a complex interplay of cytokines, T-cells, B-cells, and dendritic cells. Some of these cells have been successfully targeted in the treatment of RA by the use of biologics-based therapies. For example, rituximab therapy blocks B cell activation and abatacept effectively blocks T cell activation in patients with RA. Despite these advances, there remain some patients who are resistant to all current therapeutic options, which has encouraged further research into understanding the primary signal transduction pathways that mediate the disease. In this review we discuss the roles of the main signalling pathways, including metabolic reprogramming that have been implicated in RA disease progression, in order to develop a conceptual framework for more precise deployment of existing therapies, and to provide a rationale for producing molecular inhibitors of these pathways. Improved knowledge of the many intracellular signalling pathways in RA will complement current precision medicine strategies, particularly for the patients with difficult-to-treat RA, and especially in those with multidrug resistance disease.
Altered Neuromagnetic Activity in the Default Mode Network in Migraine and Its Subgroups (Episodic Migraine and Chronic Migraine)
Authors: Zhang Xiaofang , Xu Fengyuan , Wu Di , Wang Yingfan , Chen Qiqi , Sun Fangling , Li Yihan , Wang Xiaoshan
Published: January 2024

Background: The differences in the resting state spectral power and functional connectivity of the default mode network between people with migraine without aura (MwoA) and its subgroups differentiated by frequency (episodic migraine (EM) and chronic migraine (CM)) and healthy controls (HC) were investigated using magnetoencephalography. Methods: In the resting state, the topological spatial structure of the brain in 33 MwoA patients and 22 HC was first studied using magnetoencephalography, followed by probing the neuroelectrical activity of 17 CM and 16 EM patients, to identify damage to their default mode network (DMN). The techniques used to investigate both spectral power and functional connectivity were minimum-paradigm estimation combined with Welch’s technique and corrected amplitude envelope correlation. Results: The differences between MwoA and its subgroups (CM and EM) and HC based on spectral power were mainly in the delta, theta, and alpha bands, while the differences in functional connectivity were primarily in the delta, alpha, and beta bands. In the delta and theta bands, the spectral power of MwoA and its subgroups (CM and EM) was higher than in the HC group. The spectral power of MwoA and its subgroups (CM and EM) was lower in the alpha band. In terms of functional connectivity, the corrected amplitude envelope correlation of MwoA and its subgroups (CM and EM) was lower than the HC group in the bands with spectral differences. People with EM and CM differed in the spectral power in the left medial prefrontal cortex and the right lateral temporal cortex in the alpha band, where correlation analysis and logistic regression analysis showed that the intensity of the spectral power of the left medial prefrontal cortex was negatively correlated with headache frequency. Conclusions: The spectral power of the left medial prefrontal cortex in the alpha band may serve as a biomarker that is associated with the number of monthly headache attacks and may be a potential neuromodulatory target for controlling migraine chronicity.
Cortical Tonic Inhibition Gates the Expression of Spike-and-Wave Discharges Associated with Absence Epilepsy
Authors: Mangan Kile P. , Nelson Aaron B. , Petrou Steven , Cirelli Chiara , Jones Mathew V.
Published: January 2024

Objective: Absence seizures result from aberrant thalamocortical processing that confers synchronous, bilateral spike-and-wave discharges (SWDs) and behavioral arrest. Previous work has demonstrated that SWDs can result from enhanced thalamic tonic inhibition, consistent with the mechanism of first-line antiabsence drugs that target thalamic low-voltage-activated calcium channels. However, nearly half of patients with absence epilepsy are unresponsive to first-line medications. In this study we evaluated the role of cortical tonic inhibition and its manipulation on absence seizure expression. Methods: We used video-electroencephalogram (EEG) monitoring to show that mice with a γ-aminobutyric acid type A (GABAA) receptor mutation (γ2R43Q) display absence seizures. Voltage-clamp recordings in brain slices from wild type and γ2R43Q mice were used to evaluate the amount of tonic inhibition and its selective pharmacological modulation. Finally, we determined whether modulating tonic inhibition controls seizure expression. Results: γ2R43Q mice completely lack tonic inhibition in principal neurons of both layer 2/3 cortex and ventrobasal thalamus. Blocking cortical tonic inhibition in wild type mice is sufficient to elicit SWDs. Tonic inhibition in slices from γ2R43Q mice could be rescued in a dose-dependent fashion by the synthetic neurosteroid ganaxolone. Low-dose ganaxolone suppressed seizures in γ2R43Q mice. Conclusions: Our data suggest that reduced cortical tonic inhibition promotes absence seizures and that normal function can be restored via selective pharmacological rescue. These results, together with previous findings, suggest that deviations of tonic inhibition either above or below an optimal set point can contribute to absence epilepsy. Returning the thalamocortical system to this set point may provide a novel treatment for refractory absence epilepsy.
Current Balloon Devices for Ablation of Atrial Fibrillation
Authors: Nakahara Shiro , Hori Yuichi , Fukuda Reiko , Sato Hirotsugu , Aoki Hideyuki , Kondo Yuki , Kimura Yuta , Itabashi Yuji , Ishikawa Tetsuya , Kobayashi Sayuki , Taguchi Isao
Published: January 2024

Balloon-based catheter ablation is a valuable option for the treatment of atrial fibrillation (AF) because contiguous lesions can be created to achieve pulmonary vein isolation (PVI), and the method is less dependent than traditional ablation methods on the operator’s skill and experience. Cryoballoon ablation is used universally worldwide, with its efficacy and safety being comparable to the efficacy and safety of standard radiofrequency ablation, and the procedure can be completed in a relatively short time. Hot balloon ablation was developed in Japan. The balloon maintains its compliance even during the energy delivery, and a large areal ablation lesion is created. Furthermore, the hot balloon system is the only system for which oesophageal cooling is a standard feature. Laser balloon ablation, which is performed under direct endoscopic vision, has proven to be effective and safe for achieving a PVI. The laser balloon system provides an improved field of view and automated circumferential ablation for a rapid and effective PVI. The authors have reviewed the currently available balloon systems as used for AF ablation, i.e., PVI, and have provided detailed insight and perspectives on the currently available cryoballoon and hot balloon technologies, plus laser balloon technology.
Tarihin İzlerini Taşıyan Bir Kente Yakından Bakmak: Mimar Gözüyle Gelibolu
Authors: İNER Gülcan
Published: January 2024

Gelibolu’nun geleneksel dokusunun ele alındığı “Mimar Gözüyle Gelibolu” kitabı, Prof. Dr. Nevnihal Erdoğan ve Prof. Dr. Hikmet Temel Akarsu tarafından yazılmış ve Verita Yayınevi tarafından 2019 yılında yayımlanmıştır.

Gelibolu, stratejik konumu nedeniyle ilk çağlardan itibaren birçok yerleşime sahne olmuştur. Antik dönemlerde Khersonesos olarak adlandırılan yarımada, Eski Yunan’dan başlayarak, Perslerin, Makedonyalıların Bergama Krallığının ve Romalıların elinde bulunmuş, Bizanslıların döneminde çok önem kazanmıştır. [1] Osmanlı İmparatorluğu’nun bölgeye fethinden sonra uzun yıllar önemli bir geçit ve liman konumuna gelmiştir.

Defalarca fethedilerek farklı milletlerin yönetimine tanıklık eden bu tarihi kent, kuruluşundan bu yana barınma, beslenme, ibadet gibi farkı ihtiyaçlar doğrultusunda şekillenmeye başlayan mimari yapılaşması ile her dönemden kamusal ve sivil mimari eserler bırakmıştır. Bu mimari eserlerin bir kısmı korunabilmiş, önemli bir kısmı ise deprem, savaşlar gibi nedenlerle maalesef günümüze ulaşamamıştır.
Eyes on the Prize: Narcissism and Visual Attention to Status and Affiliation
Authors: Grapsas Stathis , Kyriazi Foteini Spantidaki , Dufner Michael
Published: January 2024

NOTE: Article in press in Personality Science. This is the accepted manuscript. It has not undergone typesetting or copy-editing. This eye tracking study tested the hypothesis that narcissists’ visual attention is motivated, focusing on two fundamental social motives: status and affiliation. We measured participants’ full narcissism spectrum (agency, antagonism, and neuroticism) via self-reports. We measured visual attention to status and affiliation images via eye-tracking in the lab. We hypothesized that narcissistic agency would relate to increased attention to status, and that narcissistic antagonism would relate to increased attention to status and decreased attention to affiliation. We formulated no hypotheses regarding narcissistic neuroticism. Results showed that only agentic narcissism was related to increased attention toward status. The three forms of narcissism were unrelated to attention to affiliation. These findings suggest that agentic narcissists’ attention is driven by an underlying status motive. More broadly, findings are consistent with the notion that visual attention expresses and maintains people’s personality traits via satisfying trait-congruent motives.
Testing Theories of Threat, Individual Difference, and Ideology: Little Evidence of Personality Based Individual Differences in Ideological Responses to Threat
Authors: Cassario Abigail , Brandt Mark John
Published: January 2024

Recent attempts to establish the veracity of the conservative shift hypothesis have failed to find supportive evidence. Instead, this work yields inconsistent results and reveals considerable individual differences in ideological responses to ecological threats. In two studies, we build upon this work in two ways. First, we test the conservative shift hypothesis across five ecological threats: unemployment, immigration, racial diversity, COVID-19, and violent crime, more than has been examined previously. Second, we test whether, in line with political personality theories, openness to experience and conscientiousness predict who is likely to shift to the right in the face of threat and who is not. In one nationally representative panel study from the Netherlands (N = 11,189) and one nationally representative repeated cross-sectional study in the U.S. (N = 9,040), we find minimal support for the conservative shift hypothesis and theories that predict personality based individual differences in ideological responses to threat.
The role of the somatosensory system in the feeling of emotions: a neurostimulation study
Authors: Giraud Michelle , Javadi Amir-Homayoun , Lenatti Carmen , Allen John , Nava Elena , Tamè Luigi
Published: January 2024

Emotional experiences deeply impact our bodily states, such as when we feel ‘anger’, our fists close and our face burns. Recent studies have shown that emotions can be mapped onto specific body areas, suggesting a possible role of the primary somatosensory cortex (S1) in emotion processing. However, the causal role of S1 in emotion generation remains unclear. To address this question, we applied transcranial Alternating Current Stimulation (tACS) on S1 at different frequencies (beta, theta and sham) while participants saw emotional stimuli with different degrees of pleasantness and level of arousal. Results showed that modulation of S1 influenced subjective emotional ratings as a function of the frequency applied. While beta-tACS made participants rate the emotional images as more pleasant (higher valence), theta-tACS lowered the subjective arousal ratings (more calming). Skin conductance responses confirmed a different arousal for pleasant vs unpleasant stimuli. Our study revealed that S1 has a causal role in the feeling of emotions, adding new insight into the embodied nature of emotions. Importantly, we provided causal evidence that beta and theta frequencies contribute differently to the modulation of two dimensions of emotions - arousal and valence - corroborating the view of a dissociation between these two dimensions.
The role of expectancies and selective interrogation of information in trait anxiety-linked affect when approaching potentially stressful future events
Authors: Reynolds Amelia , MacLeod Colin , Grafton Ben
Published: January 2024

The present study examined cognitive mechanisms underpinning high trait anxious individuals’ increased tendency to experience inflation of negative affect when approaching potential stressors. Specifically, the roles of (1) disproportionately negative relative to positive expectancies (i.e., negative expectancy bias) and (2) disproportionately interrogating negative relative to positive information (i.e., negative interrogation bias), each concerning the potential stressor, were examined. High and low trait anxious participants (N = 286) completed the experimental session, in which they were informed they may view a potentially stressful film. As participants approached the putative film viewing, participants' negative and positive affect, as well as their negative and positive expectancies were assessed. Additionally, negative interrogation bias was assessed by providing participants the opportunity to selectively interrogate information from a larger pool of negative and positive information concerning the putative film viewing. Our findings provide evidence indirect associations between trait anxiety and inflation of negative affect is serially mediated via negative interrogation bias and, in turn, negative expectancy bias. Findings are discussed with regards to limitations and potential implications for public health campaigns, and cognitive interventions for anxiety, highlighting the utility of further examining negative interrogation bias as an avenue for improving the efficacy of each.
Effects of Patient Response Pattern Distributions on Comparisons of Psychosocial Treatments for Major Depressive Disorder
Authors: Gelfand Lois , Lorenzo-Luaces Lorenzo , Dalvie Anika , Mambula Lana , DeRubeis Robert
Published: January 2024

The pharmaceutical industry has paid more attention to potential variability in depressed patients’ ability to benefit from antidepressants vs. placebo than psychotherapy researchers have when comparing more intensive to less intensive psychosocial treatments. DeRubeis et al. (2014) introduced the “patient response pattern” (PRP) as part of a framework to model variability in patients’ abilities to benefit from a single psychosocial treatment. We extend this framework to examine the influence of PRP on the effect size of a comparison of two mechanistically similar psychosocial treatments. We simulated combinations of “stronger” and “weaker” treatments, crossed with different PRP distributions. Despite modeling a standardized mean difference (SMD) of approximately 1.0, which is considered a large treatment effect, the simulation of a case mix intended to most closely model psychotherapy clinical trials resulted in an SMD of 0.22. Thus, different distributions of PRP can have a substantial effect on the ability to detect differences in the effectiveness of two treatments. Psychotherapy researchers should investigate the influence of heterogeneity in PRP distributions on the results of psychosocial treatment comparisons, and continue to investigate the possibility of using prediction of PRP (e.g., prognostic indicators) for risk stratification and tailored treatment development.
Tugas Matematika B06 Kelas 5B
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B06 Kelas 5B
Tugas Matematika B03 Kelas 5C
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B03 Kelas 5C
Tugas Matematika B04 Kelas 5C
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B04 Kelas 5C
Tugas Matematika B05 Kelas 4E
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B05 Kelas 4E
Tugas Matematika B06 Kelas 4E
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B06 Kelas 4E
Tugas Matematika B04 Kelas 5A
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B04 Kelas 5A
Tugas Matematika B04 Kelas 4D
Authors: Setiawan Adib Rifqi
Published: January 2024

Tugas Matematika B04 Kelas 4D
Growth and Yield of Cowpea (Vigna unguiculata L.) Under Different Levels of Phosphorous and Seed Inoculation with Rhizobium
Authors: Poudel Ankita , Adhikari Bishnu Bilas , Shrestha Jiban
Published: January 2024

Cowpea (Vigna unguiculata L.) is a major source of protein and an important component of Nepal's cropping systems. However, yields are very low due to a lack of improved cultivars, poor management practices, and limited input use. The objective of this study was to evaluate the effects of rhizobia inoculant and P on cowpea growth and yield. A field study was carried out in Bharatpur-11, Chitwan, Nepal, during the spring season of 2022, using a randomized complete block design with three replications and eight treatments. The treatments included four different phosphorus doses (20, 40, 60, and 80 kg ha-1) and seed inoculation (un-inoculated and inoculated). The rhizobium-inoculated plants produced significantly higher grain yield (1.27 t ha-1) and various yield-attributing parameters than un-inoculated plants. Phosphorus fertilizer at 40 kg ha-1 produced a higher grain yield (1.41 t ha-1) than other phosphorus doses. The combination of rhizobium inoculation and application of phosphorus at the rate 40 kg ha-1 yielded the highest grain yield (1.53 t ha-1). The results show that phosphorus dose of 40 kg ha-1 combined with Rhizobium inoculation with seed has the potential to improve cowpea grain yield.
Effects of Nitrogen Levels on Growth and Yield of Some Newly Released Wheat Genotypes under Bhairahawa Condition
Authors: Khatri Narayan , Pandey Bisheshwor Prasad , Rawal Nabin , Gyawali Chetan , Paneru Prakash , Pant Khem Raj , Upadhyay Chandra Prakash , Poudel Govinda Prasad , Bista Mamata
Published: January 2024

The newly released wheat varieties with different nutritional demands and their yield potential hinder generalized recommendations for nitrogen (N) fertilization. Field experiments were carried out during 2018/19 and 2019/20 growing seasons to evaluate the response of varying N fertilizer rates on growth and yield parameters of two newly released wheat varieties (Borlaug 2020 and   Zinc Gahun-1), one promising line (NL 1179) and Vijay as a check variety. Five N levels (i.e. 0, 50,100,150 and 200 kg N ha-1) were applied in the experiment designed in split plot with three replications. Nitrogen levels and genotypes were allocated as main plot and sub plot treatments, respectively. The combined analysis of both growing seasons indicated that, all newly released and promising genotypes performed better than check variety. NL 1179 recorded the highest grain yield followed by Borlaug 2020 and Zinc Gahun-1. A linear increase of grain yield was observed with increased rate of N from 0 to 200 kg ha-1, while 200 kg N ha-1showed significantly (p<0.001) highest grain yield which was statistically at par with 150 kg N/ha. Similarly, gross margin (Rs. 148933 ha-1) was highest with the application of N @ 150 kg ha-1. Interaction effect of genotypes and N levels was not found significant. Thus, it can be concluded that for higher grain production of improved wheat varieties, 150 kg Nha-1could be recommended under Bhairahawa condition.