bluuebunny/crossref_metadata_2025 huggingface dataset:
East of East
Authors: Cheng Wendy
Published: February 2015

While Los Angeles’s San Gabriel Valley is best known for being the “first suburban Chinatown,” it is actually a lively, multiethnic, and multiracial community with a complex past, and an emerging arts scene that celebrates that complexity. Today, the “SGV” constitutes the largest, majority-Asian American and Latino community in the United States. Its social and cultural mix make it a vibrant example of suburban cosmopolitanism, in which diverse residents live in relative harmony, with mutual respect for difference. Local groups such as the South El Monte Arts Posse have begun to make work that reflects this ethos, and could reshape normative ideas of what it means to be American.
Prolonged life-threatening hypoglycaemia following dose escalation of octreotide LAR in a patient with malignant polysecreting pancreatic neuroendocrine tumour
Authors: Abell Sally K , Teng Jessie , Dowling Anthony , Hofman Michael S , MacIsaac Richard J , Sachithanandan Nirupa
Published: February 2015

This paper details the case of a 77-year-old male with refractory hypoglycaemia due to inoperable metastatic pancreatic neuroendocrine tumour (pNET) co-secreting insulin and gastrin. Multiple medical therapies were trialled with limited success, and we describe the complications experienced by our patient. Somatostatin analogues can ameliorate hypoglycaemia and may have tumour-stabilising effects; however, in our case resulted in paradoxical worsening of hypoglycaemia. This rendered our patient hospital dependent for glycaemic support including continuous dextrose infusion. Although this is a reported adverse effect with initiation of therapy, we describe successful initiation of short-acting octreotide as an inpatient followed by commencement of long-acting octreotide. Hypoglycaemic collapse occurred only after dose titration of long-acting octreotide. We outline the pitfalls of somatostatin analogue therapy and the mechanisms that may contribute to worsening hypoglycaemia. This rare side effect cannot be reliably predicted, necessitating close supervision and glucose monitoring during therapy. Our patient achieved disease stabilisation and gradual resolution of hypoglycaemia with peptide receptor radionuclide therapy (PRRT), an emerging therapeutic option for metastatic neuroendocrine tumours with high efficacy and low toxicity. We present a brief but comprehensive discussion of currently available and novel therapies for insulin secreting pNETs.

Learning points

Hypoglycaemia due to malignant insulin secreting pNET is frequently severe and may be life-threatening despite supportive therapies.

Octreotide can ameliorate hypoglycaemia, and may have anti-proliferative and tumour-stabilising effects in malignant pNETs that are surgically unresectable.

Paradoxical worsening of hypoglycaemia may occur with octreotide initiation and dose titration, necessitating close supervision and glucose monitoring.

PRRT is emerging as a therapeutic option with high efficacy and low toxicity.
The Transcriptional Stress Response of Candida albicans to Weak Organic Acids
Authors: Cottier Fabien , Tan Alrina Shin Min , Chen Jinmiao , Lum Josephine , Zolezzi Francesca , Poidinger Michael , Pavelka Norman
Published: February 2015

Candida albicans is the most important fungal pathogen of humans, causing severe infections, especially in nosocomial and immunocompromised settings. However, it is also the most prevalent fungus of the normal human microbiome, where it shares its habitat with hundreds of trillions of other microbial cells. Despite weak organic acids (WOAs) being among the most abundant metabolites produced by bacterial microbiota, little is known about their effect on C. albicans. Here we used a sequencing-based profiling strategy to systematically investigate the transcriptional stress response of C. albicans to lactic, acetic, propionic, and butyric acid at several time points after treatment. Our data reveal a complex transcriptional response, with individual WOAs triggering unique gene expression profiles and with important differences between acute and chronic exposure. Despite these dissimilarities, we found significant overlaps between the gene expression changes induced by each WOA, which led us to uncover a core transcriptional response that was largely unrelated to other previously published C. albicans transcriptional stress responses. Genes commonly up-regulated by WOAs were enriched in several iron transporters, which was associated with an overall decrease in intracellular iron concentrations. Moreover, chronic exposure to any WOA lead to down-regulation of RNA synthesis and ribosome biogenesis genes, which resulted in significant reduction of total RNA levels and of ribosomal RNA in particular. In conclusion, this study suggests that gastrointestinal microbiota might directly influence C. albicans physiology via production of WOAs, with possible implications of how this fungus interacts with its host in both health and disease.
Fractal-Based Research Approach for Lignocellulose-to-Ethanol Conversion
Authors: Chi Congcong , Liu Mi , Gong Yahui , Zhang Sufeng , Zhang Bin
Published: February 2015

The microstructure of porous lignocellulose has irregularity, which represents self-similarity within the scope of a certain scale, and the conversion process of lignocellulose to bioethanol is complex. The fractal theory appears to be well suited to be an effective tool for describing and studying such irregularity and complexity. Why not introduce the fractal theory as a potentially efficient and effective way to describe the process? Here in this paper, the research development of fractal theory and its potential application in lignocellulose microstructure and enzymatic hydrolysis kinetics are discussed.
Improving Water Resistance of Soy-Protein Wood Adhesive by Using Hydrophilic Additives
Authors: Chen Minzhi , Chen Yan , Zhou Xiaoyan , Lu Bitao , He Meiping , Sun Song , Ling Xi
Published: February 2015

Soy protein adhesives are good candidates for the replacement of formaldehyde-based adhesives due to environmental concerns. However, poor water resistance has limited their application. This study was conducted to improve the water resistance of a soy-protein adhesive intended for plywood by polyethylene glycol (PEG) with different molecular weights. Ethylene glycol (EG), diethylene glycol (DEG), 400-, 2000-, and 10000-dalton polyethylene glycols were used as additives to soy protein isolate (SPI). The hydrogen bonding interaction, thermal properties, wettabilities on poplar veneer, and adhesion properties of the blended adhesives were investigated. Results showed that improving the wettability and intermolecular hydrogen bonding, induced by ethylene glycol, increased the wet adhesion strength by 30%. Higher-molecular weight polyethylene glycol imposed a decrease in adhesion due to its poor water resistance. Based on the present results, it is proposed to improve the water resistance of soy adhesives by introducing hydrophilic polyols, which also could simultaneously improve surface wetting and wet adhesion.
The Effect of Layer Moisture Content Distribution on Lumber Surface and Bonding Interface Properties
Authors: Zhang Xiaofeng , Cheng Qingzheng , Sun Liping , Xu Min , Via Brian
Published: February 2015

This study investigated the effect of drying on moisture content (MC) distribution through ash lumber thickness, as well as the effect on wood surface and bonding at the interface. After the drying of lumber, the wood surface contact angles and free energy were collected over 6 days, and the MC difference (between surface and core) was measured. At the same time, the isocyanate adhesive strength, as well as the elemental composition of carbon (C1s), nitrogen (N1s), and oxygen (O1s) on the lumber surface and at the bonding interface, were tested daily. Both the wood surface contact angle and free energy changed with a change in MC difference. The O1s concentration at the bond interface decreased with increased MC difference, and the adhesive strength declined accordingly. To attain the best bond interface, the MC difference between the surface and core should be controlled within the range of 0.5 to 1%.
Using the Hospital as a Venue for Reproductive Health Interventions: A Survey of Hospitalized Adolescents
Authors: Guss Carly E. , Wunsch Caroline A. , McCulloh Russell , Donaldson Abigail , Alverson Brian K.
Published: February 2015

Background and Objectives:

Less than one-half of sexually active adolescents have received counseling regarding contraception and sexually transmitted disease (STD) from their health care provider. We hypothesized that hospitalized adolescents would be interested in receiving reproductive health education and/or STD testing. In addition, we assessed the opinion of female adolescents on initiation of contraception during hospitalization.

Methods:

A convenience sample of 13- to 18-year-old male and female adolescent patients hospitalized at a tertiary pediatric hospital were approached for inclusion. Consenting patients completed a self-administered anonymous questionnaire.

Results:

Questionnaires from 49 female adolescents and from 51 male adolescents were collected. Based on their answers, 37% of female adolescents and 44% of male adolescents want to learn more about contraception and/or abstinence in the hospital independent of sexual activity. Thirty percent of respondents reported a desire for STD testing in the hospital. Fifty-seven percent of female subjects answered that adolescents should be offered contraception while in the hospital.

Conclusions:

Among hospitalized adolescents in this study, there was an interest in reproductive health education and contraception even among those with a primary care provider and without sexual experience. Thus, hospital-based pediatricians can play a pivotal role in expanding critical sexual health services by discussing and offering sexual health care to hospitalized adolescents. We recommend that hospitals make a brochure available to all admitted adolescents, with opportunity for follow-up discussions with their hospitalist.
Ongoing Maternal-Child Transmission of HIV in an Urban Area, 2003–2012
Authors: Rutstein Richard M. , Volkman Kathleen M. , Bonda Sirisha , Shah Samir S.
Published: February 2015

Background and Objective:

Mother-to-child transmission of HIV can be prevented by prenatal and peripartum interventions. We sought to determine the prevalence of vertical HIV transmission in an urban cohort of HIV-exposed infants and describe cases of vertical HIV infection presenting during and after the neonatal period.

Methods:

This retrospective cohort study included HIV-exposed infants born between July 1, 2003, and June 30, 2012, who received care at an urban referral site.

Results:

There were 516 infants with HIV exposure known by the time of delivery; 9 of these infants (1.7%; 95% confidence interval: 0.8%–3.3%) were HIV infected. The HIV infection rate was 0.7% for those receiving prenatal antiretroviral (ARV) therapy and 9.3% for those receiving only intrapartum and/or postnatal ARV therapy. Among those diagnosed with HIV at delivery, 46% received no prenatal care.

Conclusions:

Our data suggest that strategies to eliminate infant HIV infections ought to include ensuring better access to prenatal care, HIV testing, and ARV therapy initiation during pregnancy.
Cytotoxic and toxicological effects of phthalimide derivatives on tumor and normal murine cells
Authors: FERREIRA PAULO MICHEL PINHEIRO , COSTA PATRICIA MARÇAL DA , COSTA ARINICE DE MENEZES , LIMA DAISY JEREISSATI BARBOSA , DRUMOND RENATA ROSADO , SILVA JURANDY DO NASCIMENTO , MOREIRA DIOGO RODRIGO DE MAGALHÃES , OLIVEIRA FILHO GEVÂNIO BEZERRA DE , FERREIRA JAMILE MAGALHÃES , QUEIROZ MARIA GORETTI RODRIGUES DE , LEITE ANA CRISTINA LIMA , PESSOA CLÁUDIA
Published: February 2015

Eleven phthalimide derivatives were evaluated with regards to their antiproliferative activity on tumor and normal cells and possible toxic effects. Cytotoxic analyses were performed against murine tumors (Sarcoma 180 and B-16/F-10 cells) and peripheral blood mononuclear cells (PBMC) using MTT and Alamar Blue assays. Following, the investigation of cytotoxicity was executed by flow cytometry analysis and antitumoral and toxicological potential by in vivo techniques. The molecules 3b, 3c, 4 and 5 revealed in vitro cytotoxicity against Sarcoma 180, B-16/F-10 and PBMC. Since compound 4 was the most effective derivative, it was chosen to detail the mechanism of action after 24, 48 and 72 h exposure (22.5 and 45 µM). Sarcoma 180 cells treated with compound 4 showed membrane disruption, DNA fragmentation and mitochondrial depolarization in a time- and dose-dependent way. Compounds 3c, 4 and 5 (50 mg/kg/day) did not inhibit in vivotumor growth. Compound 4-treated animals exhibited an increase in total leukocytes, lymphocytes and spleen relative weight, a decreasing in neutrophils and hyperplasia of spleen white pulp. Treated animals presented reversible histological changes. Molecule 4 had in vitro antiproliferative action possibly triggered by apoptosis, reversible toxic effects on kidneys, spleen and livers and exhibited immunostimulant properties that can be explored to attack neoplasic cells.
Práticas sociais das perversões: modernidade do laço, organização social e dilemas morais
Authors: Lévy Alexandre
Published: February 2015

Propomos estudar primeiramente certos aspectos do discurso capitalista, aproximando-os dos processos perversos da clínica do sujeito para, em seguida, questionar a dimensão da moral e da construção social. Apoiaremo-nos em algumas pesquisas experimentais sobre a submissão à autoridade, bem como sobre algumas questões postas pela filosofia moral em termos de "dilemas", a fim de avaliar os efeitos de divisão subjetiva. A orientação perversa parece ser aqui um motor essencial, fazendo passar, em nome do Outro, um gozo que não diz seu nome, um gozo desmentido.
Les temps pour comprendre des "petits curieux"
Authors: Pautrel Véronique
Published: February 2015

À partir de plusieurs situations cliniques de consultations psychothérapeutiques, le constat d'une contradiction entre la disqualification des recherches sexuelles de l'enfant par les adultes alors même que ces derniers hyper-sexualisent l'environnement permet de soutenir un questionnement sur les effets psychiques de cette contradiction sociale sur le développement psycho affectif de l'enfant. Que devient la curiosité de l'enfant quand le temps pour comprendre disparaît?
Perfil dos Psicólogos Inscritos na Subsede Leste do CRP-04
Authors: Santos Keicy Rocha , Monteiro Líbia Gomes , Torres Maria Luiza Camargo , Sousa Leonardo Gomes de , Coelho Adilson Rodrigues
Published: February 2015

O objetivo deste estudo foi fazer um levantamento do perfil profissional dos psicólogos inscritos na Subsede Leste do Conselho Regional de Psicologia nº 04 (CRP-04), atuantes no município de Governador Valadares até 2010. Trata-se de um estudo quantitativo e comparativo entre os dados da presente pesquisa e aqueles que compõem o banco nacional, que se encontra na obra O trabalho do Psicólogo no Brasil, organizado por Bastos, Gondim e colaboradores (2010). Utilizou-se um questionário via e-mail marketing, respondido por 85 participantes que abarcaram as categorias do perfil profissional. Os dados levantados foram tabulados no programa Microsoft Excel e analisados no programa Sphinx Survey Edição Léxica. Verificou-se que a maioria dos participantes é branca, casada, do gênero feminino, com idade entre 26 e 30 anos e graduada em instituição privada, tendo especialização, principalmente em clínica organizacional e atuação na clínica. Ganha entre 3 e 4 salários mínimos como psicólogo, na condição de assalariado. Geralmente, a maioria não possui outra atividade profissional e a renda familiar é de, aproximadamente, 10 salários mínimos. Os dados corroboram com a média nacional do perfil profissional dessa classe de trabalhadores, trazendo algumas peculiaridades do município de Governador Valadares.
Acompanhamento Terapêutico e a Relação Mãe-Bebê
Authors: Engel Daiane , Ghazzi Mercês Sant'Anna , Silva Heloisa Cardoso da
Published: February 2015

O presente estudo busca refletir sobre a clínica do Acompanhamento Terapêutico (AT), indagando sobre a pertinência dessa intervenção junto a famílias de bebês que apresentem risco em sua estruturação psíquica. Para tal, realizou-se uma investigação teórica sobre o AT e sobre a clínica com bebês, os quais serviram de base para a análise dos fragmentos de um caso de AT, realizado com um bebê e sua mãe, junto ao Pró-bebê, o qual é um projeto de extensão que acompanha o desenvolvimento de crianças de zero a três anos em um posto de saúde de Torres/RS. A partir desses relatos, discutiu-se essa forma de intervenção, buscando comprovar a sua eficácia no atendimento a famílias com bebês que apresentem risco para seu desenvolvimento ou estruturação psíquica.
Crítica, razão e sociedade: convergência e paralelismo entre Foucault e Adorno & Horkheimer
Authors: Hilário Leomir Cardoso , Cunha Eduardo Leal
Published: February 2015

Este artigo parte do ponto de vista defendido por Axel Honneth, no qual Foucault e Habermas são compreendidos como desenvolvimentos rivais do campo problemático aberto pela Escola de Frankfurt, Adorno e Horkheimer em particular. Propomos que é a conduta diante do Iluminismo que afasta Habermas e Honneth de Foucault e também de Adorno e Horkheimer. Tecemos, assim, uma costura entre Foucault e a primeira geração da Escola de Frankfurt, que se apoia inicialmente na crítica de Honneth tanto a um quanto a outro, para em seguida ultrapassá-la, mostrando, ao mesmo tempo, os pontos em comum – centrados na crítica radical da razão – e os motivos para considerá-los responsáveis por empreitadas filosóficas distintas.
Afirmar uma Psicologia fortalecedora da vida com Nietzsche e Deleuze
Authors: Yonezawa Fernando Hiromi
Published: February 2015

Neste estudo teórico, desejamos compreender a noção trazida por Nietzsche, de Psicologia como "morfologia da vontade de poder" e, também, investigar o problema filosófico destacado por Deleuze de liberar a vida do par ressentimento/má-consciência. Trazemos os conceitos de vontade de poder e eterno retorno como sendo elementos de transformação do enfraquecimento que a vida sofre sob obra do ressentimento e da má-consciência. Assim, chegaremos à noção de que a Psicologia pode funcionar como estudo de uma sensibilidade das forças vitais, trabalhando em favor de fortalecer a vida, liberando-a do peso da culpa, e sem se restringir à interioridade dos sujeitos.
Fungos micorrízicos arbusculares em campos de murundus após a conversão para sistemas agrícolas no cerrado
Authors: Assis Paula Camylla Ramos , Saggin Júnior Orivaldo José , Paulino Helder Barbosa , Stürmer Sidney Luiz , Siqueira José Oswaldo , Carneiro Marco Aurélio Carbone
Published: February 2015

Os campos de murundus são fitofisionomias de ocorrência no bioma Cerrado com funções ecológicas importantes para a manutenção da sustentabilidade do solo; e a conversão para sistemas agrícolas pode provocar alterações nos atributos físicos, químicos e biológicos do solo ainda não avaliados, como a redução da biodiversidade de fungos micorrízicos arbusculares. O objetivo deste estudo foi avaliar como a conversão dos campos de murundus em áreas de sistema agrícola altera a comunidade de fungos micorrízicos arbusculares (FMAs). Os tratamentos constituíram-se de três áreas agrícolas submetidas ao mesmo manejo e uso agrícola em uma cronossequência (7, 11 e 14 anos) e duas na área referência [campo de murundus, em topo (TM) e entre os murundus (EM)]. Os esporos de FMAs foram extraídos, contados, e as espécies de FMAs identificadas pelas características morfológicas. O total de FMAs recuperado foi de 27 espécies, sendo nove espécies da família Acaulosporaceae, uma Ambisporaceae, sete Glomeraceae, duas Claroideoglomeraceae e oito Gigasporaceae. Desse total, as espécies Acaulospora scrobiculata, Glomus macrocarpum, e Gigaspora sp. ocorreram em todas as áreas nos dois anos estudados. As espécies Acaulospora mellea, Acaulospora cavernata, Acaulospora colombiana, Glomus diaphanum, Scutellospora reticulata e Scutellospora sp. só foram encontradas nos campos de murundus. A conversão de campos de murundus em área agrícola modificou a ocorrência e composição da comunidade de FMAs; as espécies Acaulospora scrobiculata, Glomus macrocarpum, Claroideoglomus etunicatus e Gigaspora sp ocorreram em todas as áreas e a não ocorrência de algumas espécies nas áreas de cultivo, como as espécies Acaulospora cavernata, Acaulospora colombiana, Rhizophagus diaphanus, Scutellospora reticulata e Scutellospora sp. representa perda de diversidade desses fungos. Portanto, este estudo tratou-se do primeiro relato da ocorrência e da estrutura da comunidade de FMAs em fitofisionomia de campos de murundus, contribuiu para o maior entendimento dos FMAs no bioma Cerrado e demonstrou que as alterações promovidas pela conversão da área alteraram a ocorrência e a diversidade dos fungos micorrízicos arbusculares.
Produção de inoculante micorrízico on farm utilizando resíduos da indústria florestal
Authors: Czerniak Marlon João , Stürmer Sidney Luiz
Published: February 2015

A produção de inoculante à base de fungos micorrízicos arbusculares (FMAs) utilizando o método on farm é uma alternativa para estimular o uso de inoculante microbiano no sistema de produção vegetal e reduzir os custos associados com a compra desse produto. O objetivo deste estudo foi avaliar o potencial de resíduos do setor florestal, a casca de Pinus (CP) e o lodo de celulose (LC), como componentes do substrato para a produção de inoculante micorrízico on farm . Plantas de sorgo pré-colonizadas com os FMAs, Claroideoglomus etunicatum RJN101A e Dentiscutata heterogama PNB102A, foram estabelecidas em casa de vegetação por três meses em substrato formado por areia:argila expandida:solo (2:2:1). Após, essas foram transplantadas para sacos plásticos de 20 L contendo substrato formado por CP ou LC, misturados com casca de arroz carbonizada + solo de barranco (1:1:1). O experimento seguiu um fatorial 2 × 2, sendo dois isolados fúngicos e dois resíduos, com cinco repetições, em delineamento inteiramente casualizado. As plantas cresceram por três meses sob condições ambientais e, após esse período, o substrato foi analisado quanto ao número de esporos de FMAs, à colonização micorrízica da planta hospedeira e ao potencial de inóculo pelo método do NMP (número mais provável). O substrato foi dividido em três camadas (superior, mediana e inferior) e apenas o número de esporos foi avaliado individualmente para cada camada. O número de esporos de ambos FMAs não foi influenciado pelo tipo resíduo, mas diminuiu da parte superior para a inferior dentro de cada unidade experimental. D. heterogama tendeu a produzir maior número de esporos do que C. etunicatum . A porcentagem de colonização micorrízica do sorgo pelos FMAs foi significativamente maior no resíduo LC do que CP. O número de propágulos infectivos de FMAs tendeu também a ser maior em LC (22 a 28 propágulos cm-3 substrato) do que em CP (1,6 a 6,5 propágulos cm-3 substrato). O resíduo LC tem potencial para ser utilizado como componente do substrato em sistemas de produção de inoculante micorrízico on farm.
Limite crítico no solo para transpiração e crescimento foliar em mandioca em dois períodos com deficiência hídrica
Authors: Pinheiro Diego Garrido , Streck Nereu Augusto , Richter Gean Leonardo , Langner Josana Andreia , Winck José Eduardo Minussi , Uhlmann Lilian Osmari , Zanon Alencar Junior
Published: February 2015

A cultura da mandioca geralmente é exposta a mais de um período de deficiência hídrica durante a estação de crescimento. O objetivo deste trabalho foi verificar se há diferença na Fração de Água Transpirável do Solo (FATS) crítica para transpiração e crescimento foliar em plantas de mandioca submetidas a um e dois períodos de deficiência hídrica no solo. Foram conduzidos dois experimentos com a cultura da mandioca, cultivar Fepagro RS 13. Os tratamentos foram quatro regimes hídricos subdivididos em dois períodos, período 1 (P1) e período 2 (P2): regimes hídricos RH1 e RH2 (sem e com deficiência hídrica nos dois períodos, respectivamente) e regimes hídricos RH3 e RH4 (com deficiência hídrica no P1 e P2, respectivamente). Usou-se o método da FATS para indicar os pontos críticos para transpiração e crescimento foliar. A FATS crítica foi 0,35, 0,38 e 0,37 para crescimento foliar e 0,28; 0,26; e 0,28 para transpiração no P1 do RH2 e RH3 e P2 do RH4, respectivamente. No P2 do RH2, a FATS crítica para crescimento foliar e transpiração foi 0,09 e 0,13, respectivamente. Concluiu-se que há diminuição na FATS crítica em mandioca no segundo período comparado ao primeiro período de deficiência hídrica no solo, que pode ser explicada pela menor área foliar, o que permitiu que o período de turgescência das folhas fosse maior e, com isso, demoraram mais a ativar seus mecanismos de controle estomático. A implicação prática desses resultados é que esses podem ser utilizados como parâmetros para irrigação da cultura, e também, na seleção de cultivares mais tolerantes à deficiência hídrica.
Estudo da atividade anti-helmíntica do extrato etanólico de Jatropha mollissima (Pohl) Baill. (Euphorbiaceae) sob Haemonchus contortus em ovinos no semiárido paraibano
Authors: Ribeiro Ana R.C. , Andrade Fábio D. de , Medeiros Maria do C. de , Camboim Alricélia da S. , Pereira Júnior Francisco A. , Athayde Ana C.R. , Rodrigues Onaldo G. , Silva Wilson W.
Published: February 2015

Objetivou-se com o presente trabalho, avaliar o efeito anti-helmíntico de Jatropha mollissima por meio de experimentos in vitro e in vivo. Inicialmente foi investigada a concentração de extrato com efeito bioativo, pelo teste de evolução da toxicidade do extrato etanólico de J. mollissima sobre o microcrustáceo Artemia salina, obtendo uma CL50 de 660,80µg/ml, que foi testada em coproculturas contendo larvas infectantes de Haemonchus contortus e em animais para a verificação da redução do OPG. Para o teste in vivo o extrato foi dissolvido em água para se obter as concentrações 660,80µg/ml e 1321,6µg/ml, foram coletadas fezes semanalmente e sangue quinzenalmente. Como resultados dos testes in vitro, o extrato etanólico do caule de Jatropha mollissima mostrou-se tóxico sobre A. salina, com CL50 abaixo de 1000 µg/ml e inibiu a eclosão de ovos e o desenvolvimento de larvas de H. contortus, apresentando uma eficiência de 70,77%. O teste in vivo revelou que o extrato é também eficaz em ovinos, com redução significativa na contagem de OPG após 28 dias de experimento, 47 e 44% de redução nos grupos tratados com o extrato, 7,5% no grupo de animais não tratados e 40,6% com a ivermectina. Mesmo parasitados, os animais permaneceram clinicamente saudáveis e sem anemia. O extrato etanólico do caule de Jatropha mollissima pode representar uma alternativa ao controle da verminose ovina, pois retarda a resistência parasitária.
Evaluation of a MPB70-ELISA to differentiate Mycobacterium bovis from M. avium-sensitized swine
Authors: Marassi Carla D. , Oliveira Flávia C.S. de , Pinheiro Sonia R. , Azevedo Sergio S. , Soto Francisco R.M. , Oelemann Walter , Lilenbaum Walter , Vasconcellos Silvio A.
Published: February 2015

Swine are susceptible to different mycobacteria species, being Mycobacterium bovis an agent of tuberculosis, with most significant zoonotic risks, while M. avium determines a granulomatous lymphadenitis with low zoonotic risk. Currently performed intradermal tests present some important limitations, such as the lack of ability to detect anergic animals or to differentiate among mycobacterial species. In order to improve the TB diagnosis, serological assays have been developed, with encouraging results. The purpose of this study was to evaluate the performance of a MPB70-ELISA in 82 piglets divided into four groups: sensitized by inactivated M. bovis, M. avium, inoculated with oil adjuvant, or with saline solution. The test was able to discriminate between an animal sensitized by M. bovis and animals of the three other groups, including M. avium-sensitized animals; for this reason, we suggest that MPB70-ELISA could be used as a complementary tool for discriminating the agent of the mycobacteriosis, and therefore to diagnose tuberculosis in a swine herd.
Lead poisoning in cattle and chickens in the state of Pará, Brazil
Authors: Barbosa José Diomedes , Bomjardim Henrique dos Anjos , Campos Karinny Ferreira , Duarte Marcos Dutra , Bezerra Júnior Pedro Soares , Gava Aldo , Salvarani Felipe Masiero , Oliveira Carlos Magno Chaves
Published: February 2015

The present study describes the occurrence of lead poisoning in cattle and chickens in Pará, Brazil. In a lot composed of 80 calves from a dairy herd, 10 animals became sick and nine died, but one animal recovered after being removed from the paddock. Upon inspection of this paddock, the presence of truck batteries used to store energy captured by solar panels was found. The clinical signs observed in calves included difficult breathing, nasal discharge, excessive salivation, corneal opacity, pushing of the head against objects and recumbency. The chickens had decreased oviposition and produced eggs with thin or malformed shells. The necropsy findings of the cattle, as well as the histopathological changes observed, were of little significance except for one animal that showed mild astrocytosis histopathology in the cerebral cortex. In one of the chickens, renal histopathology showed mild multifocal acute tubular necrosis. The mean lead concentrations in the livers and kidneys of the cattle were 93.91mg/kg and 209.76mg/kg, respectively, and the mean concentration detected in chicken livers was 105.02mg/kg. It was concluded that the source of lead poisoning in these calves and chickens were the truck battery plates, which were within reach of the animals.
Elimination of the tremorgenic toxin of Ipomoea asarifolia by milk
Authors: Lopes José Radmácyo G. , Riet-Correa Franklin , Cook Daniel , Pfister James A. , Medeiros Rosane M.T.
Published: February 2015

With the aim to determine if the tremorgenic toxin of Ipomoea asarifolia is eliminated in milk, three groups of Swiss female mice received, immediately after giving birth until weaning, a ration containing 20% or 30% of dry I. asarifolia. All the offspring of the females that received 20% or 30% I. asarifolia showed tremors 2-4 days after birth. The offspring of the females that received 20% I. asarifolia recovered 4-7 days after weaning. The offspring of the females that received 30% of the plant in the ration died while showing tremors before weaning or up to two days after weaning. It is concluded that the tremorgenic compound of I. asarifolia or its toxic metabolites are eliminated in milk, and that lactating mice may be used as a model for the determination of the toxic compound(s) in this plant.
Clinical and parasitological evaluation of pour-on fluazuron and ivermectin for treating canine demodicosis
Authors: Souza Clarissa P. , Ramadinha Regina H.R. , Scott Fabio B.
Published: February 2015

The objective of the study was to evaluate the efficacy of pour-on formulations of fluazuron and ivermectin in different therapeutic protocols for treatment of demodicosis by means of quantifying mites with skin scraping, histological and clinical evaluation in dogs. Eighteen dogs with skin scrapings positive for Demodex canis were evaluated, divided into three groups. All the animals were treated every 14 days, completing 6 treatments for each animal (days 0, 14, 28, 42, 56 and 70). In group 1, pour-on 2.5% fluazuron was used at the dose of 20mg/kg; in the group 2 pour-on 2.5% fluazuron at a dose of 20 mg/kg in association with pour-on 0.5% ivermectin at the dose of 0.6mg/kg; and in group 3, pour-on 0.5% ivermectin alone was used, at the dose of 0.6mg/kg. The treatment was evaluated and monitored through skin scrapings and clinical follow-up of the lesions every 14 days for 84 days, and through histopathological examination at the end of each treatment protocol. The success rate was defined as the percentage of dogs in each group that had negative skin scrapings after the treatment: this was 16.67% for group 1, and 50% for groups 2 and 3. The reduction in mite counts reached effectiveness of 67.66%, 88.99% and 84.29% for groups 1, 2 and 3 respectively. The Wilcoxon test showed that there was a significant difference between the number of mites before and after treatment in groups 2 and 3. The histopathological examination revealed that only group 1 showed no significant difference in the intensity of infestation between days 0 and 84. Clinically, there was no significant difference between the evaluation before and after treatment in the three groups. pour-on 2.5% fluazuron and pour-on 0.5% ivermectin were not effective for treating canine demodicosis, either in association or as single therapy, when applied every 14 days for a period of 70 days. Quantification of mites using skin scrapings and histological evaluation proved to be ineffective, either one as sole therapeutic evaluation parameters, for canine demodicosis.
Fatal Human herpesvirus 1 (HHV-1) infection in captive marmosets (Callithrix jacchus and Callithrix penicillata) in Brazil: clinical and pathological characterization
Authors: Casagrande Renata A. , Pannuti Cláudio S. , Kanamura Cristina , Freire Wilton S. , Grespan André , Matushima Eliana R.
Published: February 2015

Fatal Human herpesvirus 1 (HHV-1) was diagnosed in 12 captive marmosets (Callithrix jacchus and Callithrix penicillata) from metropolitan region of São Paulo, São Paulo State. Clinical signs were variable among the cases, but most affected marmosets presented signs associated with viral epithelial replication: oral, lingual and facial skin ulcers and hypersalivation, and viral replication in the central nervous system: prostration, seizure and aggressive behavior. Consistent microscopic findings were diffuse mild to severe nonsuppurative necrotizing meningoencephalitis with gliosis, vasculitis and neuronal necrosis. Additionally, in the brain, oral cavity, skin, adrenal gland and myoenteric plexus intranuclear inclusion bodies were present. Immunohistochemistry confirmed the presence of the HHV-1 antigen in association with lesions in the brain, oral and lingual mucosa, facial skin, adrenal gland and myoenteric plexus. HHV-1-specific polymerase chain reaction (PCR) analysis of the brain was carried out and the virus was detected in 7/8 infected marmosets. It is concluded that HHV-1 causes widespread fatal infection in marmosets.
Spatial-temporal distribution and recruitment of Stramonita haemastoma (Linnaeus, 1758) (Mollusca) on a sandstone bank in Ilhéus, Bahia, Brazil
Authors: Santos JJB. , Boehs G.
Published: February 2015

We examined aspects of the population ecology of the gastropod Stramonita haemastoma at Ilhéus, Bahia, Brazil. We collected monthly from October 2007 through September 2008 on a sandstone bank 1.5 km long, on which two points and two sampling levels were defined. We took five previously randomised replicates at each level, using a square enclosing an area of 0.0625 m². The snails were photographed, counted, and measured at the site. Both recruits and other individuals in the population preferentially inhabit the regions closer to the lower shore, where they are submerged for longer periods and are less subject to physiological stresses caused by temperature variation and desiccation. The cracks and holes in the bank serve as refuges and places for S. haemastoma to lay their egg capsules. Recruits were observed throughout the study period, and the population showed continuous reproduction.
Importance of lateral lagoons for the ichthyofauna in a large tropical reservoir
Authors: Ferrareze M. , Nogueira MG.
Published: February 2015

This study aimed to analyse the composition and the ecological attributes of small-sized fish assemblages in four lagoons and in the main channel of Rosana Reservoir (SE Brazil). Fieldwork was carried out in September and November/2004 and January, March, May and August/2005. In each sampling station and period five manual throws were performed towards aquatic macrophyte stands, using a hand net (1.5 × 5 m; 0.3 cm of mesh size). The ichthyofauna collected was represented by 42 species, totalizing 3,424 individuals, 2,186 g in weight. The order Characiformes was dominant, mainly in the lagoons with low connectivity with the river. The main taxon (Importance Index) was Hemigrammus marginatus. Higher richness, abundance, biomass, diversity and lower individual mean length were observed in the lagoons, especially during the rainy period. Spatial segregation of some species was showed by the canonical correspondence analysis indicating the habitat complexity. The results validate the hypotheses that lateral lagoons have a prominent ecological role in the life cycle of juveniles and small fish and demonstrate how the connectivity river/lagoons may be important for assemblages maintenance.
Impact of maintenance of Macrobrachium rosenbergii De Man, 1879 (Crustacea, Decapoda, Palaemonidae) broodstock on the water used in culture ponds
Authors: Biudes JFV. , Camargo AFM. , Henares MNP.
Published: February 2015

Aquaculture production generates social and economic benefits, but can also cause environmental impacts. The objectives of this study were: a) to characterise the impacts caused by the maintenance of broodstock of the giant river prawn (Macrobrachium rosenbergii) on the physical and chemical characteristics of the water used in culture ponds, and b) to evaluate the relationship between the biomass of the prawns and the impact of culture on the water used in the ponds. Between January and December 2004, we determined, monthly, the biomass of M. rosenbergii by means of biometrics, and the physical and chemical variables of the supply and effluent water from a pond used to maintain breeding stock. The results showed that the effluent water had higher contents of chlorophyll-a, suspended particulate matter (SPM), pH, dissolved oxygen, total Kjeldahl nitrogen (TKN) and dissolved Kjeldahl nitrogen (DKN), inorganic nitrogen (IN), total (TP) and dissolved phosphorus (DP), and P-orthophosphate than the supply water. The highest biomass of M. rosenbergii occurred in April (127.0 g.m-2) and the lowest in August (71.5 g.m-2), and there were positive linear correlations between the biomass of the prawns and the intensity of the increases in TKN, DKN, IN, TP, and DP of the water used in the pond. The maintenance of broodstock of M. rosenbergii increased the chlorophyll-a, SPM, nitrogen, and phosphorus contents of the water in the pond. Additionally, the increase in the biomass of the prawns intensifies the export of nitrogen and phosphorus from the pond in the effluent.
The influence of population structure and reproductive aspects of the genus Stellifer (Oken, 1817) on the abundance of species on the southern Brazilian coast
Authors: Rodrigues-Filho JL. , Verani JR. , Peret AC. , Sabinson LM. , Branco JO.
Published: February 2015

The differences between abundance and the relationship with aspects of population and reproductive Stellifer rastrifer, Stellifer stellifer and Stellifer brasiliensis were analysed. Data were collected monthly trawl directed for capture of seabob shrimp in Armação do Itapocoroy, an important fishing area on the southern Brazilian coast. The chi-square test showed that the population of S. rastrifer presented values of capture significantly higher than others in all evaluated periods. The frequency distribution of total length curves combined with records of the size at first maturity (L50) showed that S. rastrifer is a species with a majority of the adults effectively participating in the reproductive period. The frequency of occurrence of individuals in reproduction monthly examined together with changes in the gonadosomatic index (GSI) and the reproductive activity index indicated that spring was the main breeding season for the three species. However, it was observed that the reproductive period of S. rastrifer was more pronounced and more extensive than that of its congeners, apparently providing it with ecological advantages and enabling a more effective population balance given the pressure exerted by fishing in the study area.
Simulation of the electrophoresis of amino acids in microcmputer
Authors: Fernandes et al J. R.
Published: February 2015

Abstract of the panel presented at the annual meeting of SBBQ (1993).
The impact of undergraduate research training (URT) on the scientific education os students
Authors: Aguiar L. C.C.
Published: February 2015

Abstract of the panel presented at the annual meeting of SBBQ (1996).
The didatic transposiction os the human digestion conpcet
Authors: De Souza N. G.S. , Wortmann M. L. , Souza D. O.
Published: February 2015

Abstract of the panel presented at the annual meeting of SBBQ (1996).
Oxidative phosporylation: a computer animation
Authors: Shinya L. A.M. , Galembeck E. , Torres Bayardo Baptista
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see the attachment).
A software evaluation
Authors: Galembeck E. , Macedo D. V. , Torres Bayardo Baptista
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see the attachment).
A quantitative and qualitative study of the scientific production of the Unisersidade Federal do Espirito Santo (ES, Brazil) during the period from 1990 to 1996.
Authors: Cerqueira L. , Cerqueira V. , Campos E. G.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see Annex)(see attachament).
Oxygen free radicals: an introductory software
Authors: Yokaichiya D. K. , Galembeck E. , Torres Bayardo Baptista
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see Annex)(see attachament).
Continuing educationg course for high school teachers: an approach to updating on new technology and advances in science
Authors: Barretto et al. I. F.C.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Biochemistry of water: a multidisciplinar approach
Authors: Pinto L. M. A.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
The student perception of a good teacher
Authors: Pereira M. R. , Foguel D.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see Attachment).
Biochemistry of drugs: a discipline for undergraduate students
Authors: Netto A. P.C.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Factors affecting yeast fermentation as a tool for introducing high school students to Biochemistry research
Authors: Bezerra et al. V. F.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
An easy and integrative (theoretical/ practical) Biochemical class for teaching of metabolism
Authors: Alves et al. A. A.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Correlation between MSc./ Ph.D and scientific production in Brazil
Authors: Leta J. , De Meis L.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Using the Web to provide support material in Biochemistry teaching
Authors: Dórea et al. F. C.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Biochemistry of Cancer: a discipline for undergraduates
Authors: Trigoni Jr. et al. R,
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Developing mental models in Biochemistry: an experience involving molecular models
Authors: Falcão et al. R. A.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
A new educational software on GPC tecnhique
Authors: Marson G. A. , Torres Bayardo Baptista
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
A model for evaluation of a distance education discipline
Authors: Yokaichiya D. K. , Galembeck E. , Torres Bayardo Baptista
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Avaliação das potencialidades de um curso prático de Bioquímica na implementação do ensino teórico em cursos profissionalizantes
Authors: Regis Wilian C.B. , Conçanves Maria Lúcia G.S. , Santoro Marcelo M.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
A study on the origins of studetns misconceptions about energy-yielding metabolism
Authors: Oliveira G. A. , Da Poian A. T. , Luz M. R.M.
Published: February 2015

Abstract of the panel presented at the SBBq annual meeting (see attachament).
Carbohydrates Through Animation: Preliminary Step
Authors: Sugai J.K. , Figueiredo M.S.R. , Antônio R.V. , Oliveira P.M , Cardoso V.A , Ricardo J , Merino E. , Figueiredo L.F. , Heidrich D.N.
Published: February 2015

Methods of education are changing, so the educational tools must change too. The developmentof the systems of information and communication gave the opportunity to bring new technology tothe learning process. Modern education needs interactive programs that may be available to theacademic community, in order to ease the learning process and sharing of the knowledge. Then,an educational software on Carbohydrates is being developed using concept maps and FLASH-MXanimations program, and approached through six modules. The introduction of Carbohydrates wasmade by the module Carbohydrates on Nature, which shows the animations gures of a teacher andstudents, visiting a farm, identifying the carbohydrates found in vegetables, animals, and microor-ganisms, integrated by links containing short texts to help understanding the structure and functionof carbohydrates. This module was presented, as pilot experiment, to teachers and students, whichdemonstrated satisfaction, and high receptivity, by using animation and interactivitys program asstrategy to biochemistrys education. The present work is part of the project Biochemistry throughanimation, which is having continuity.