bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic thoracic pain after cardiac surgery: role of inflammation and biomechanical sternal stability
Authors: Madjarov Jeko M. , Katz Michael G. , Hadas Yoav , Kim Sofia Jisoo , Freage-Kahn Lina , Madzharov Svetozar , Vincek Adam , Madjarova Sophia J. , Seidman Piers , Shtraizent Nataly , Robicsek Steven A. , Eliyahu Efrat
Published: August 2023

Introduction

The pathogenesis of chronic chest pain after cardiac surgery has not been determinate. If left untreated, postoperative sternal pain reduces the quality of life and patient satisfaction with cardiac surgery. The purpose of the study was to examine the effect of chest inflammation on postoperative pain, risk factors for chronic pain after cardiac surgery and to explore how chest reconstruction was associated with the intensity of pain.

Methods

The authors performed a study of acute and chronic thoracic pain after cardiac surgery in patients with and without sternal infection and compared different techniques for chest reconstruction. 42 high-risk patients for the development of mediastinitis were included. Patients with mediastinitis received chest reconstruction (group 1). Their demographics and risk factors were matched with no-infection patients with chest reconstruction (group 2) and subjects who underwent conventional sternal closure (group 3). Chronic pain was assessed by the numeric rating scale after surgery.

Results

The assessment of the incidence and intensity of chest pain at 3 months post-surgery demonstrated that 14 out of 42 patients across all groups still experienced chronic pain. Specifically, in group 1 with sternal infection five patients had mild pain, while one patient experienced mild pain in group 2, and eight patients in group 3. Also, follow-up results indicated that the highest pain score was in group 3. While baseline levels of cytokines were increased among patients with sternal infection, at discharge only the level of interleukin 6 remained high compared to no infection groups. Compared to conventional closure, after chest reconstruction, we found better healing scores at 3-month follow-up and a higher percentage of patients with the complete sternal union.

Conclusions

Overall, 14 out of 42 patients have chronic pain after cardiac surgery. The intensity of the pain in mediastinitis patients significantly decreased at 3 months follow-up after chest reconstruction. Thus, post-surgery mediastinitis is not a determining factor for development the chronic chest pain. There is no correlation between cytokines levels and pain score except interleukin 6 which remains elevated for a long time after treatment. Correlation between sternal healing score and chronic chest pain was demonstrated.
Oral fibroblasts rescue osteogenic differentiation of mesenchymal stem cells after exposure to Zoledronic acid in a paracrine effect
Authors: Berg Tal , Doppelt-Flikshtain Ofri , Coyac Benjamin R. , Zigdon-Giladi Hadar
Published: August 2023

Background: Medication-related osteonecrosis of the jaw is a serious complication that develops in oncologic patients treated with Zoledronic acid. Although used for over 30 years, the influence of Zoledronic acid on bone has been thoroughly investigated, mainly on osteoclasts. While decreasing osteoclast differentiation and function, for many years it was thought that Zoledronic acid increased osteoblast differentiation, thus increasing bone volume. Moreover, despite the influence of soft tissue on the bone healing process, the impact of zoledronic acid on the interaction between soft tissue and bone was not investigated.

Aim: Our goal was to investigate the influence of Zoledronic Acid and soft tissue cells on osteogenic differentiation of mesenchymal stem cells (MSCs).

Materials and methods: Osteogenic differentiation of MSCs was examined after exposure to Zoledronic Acid. To determine the influence of soft tissue cells on MSCs’ osteogenic differentiation, conditioned media from keratinocytes and oral fibroblasts were added to osteogenic medium supplemented with Zoledronic Acid. Proteomic composition of keratinocytes’ and fibroblasts’ conditioned media were analyzed.

Results: Zoledronic Acid decreased osteogenic differentiation of MSCs by seven-fold. The osteogenic differentiation of MSCs was restored by the supplementation of fibroblasts’ conditioned medium to osteogenic medium, despite Zoledronic acid treatment. Five osteogenic proteins involved in the TGFβ pathway were exclusively identified in fibroblasts’ conditioned medium, suggesting their role in the rescue effect.

Conclusion: Oral fibroblasts secrete proteins that enable osteogenic differentiation of MSCs in the presence of Zoledronic Acid.
Role of heparanase in pulmonary hypertension
Authors: Wang Lin-Jun , Feng Fei , Li Jian-Chun , Chen Ting-Ting , Liu Li-Ping
Published: August 2023

Pulmonary hypertension (PH) is a pathophysiological condition of increased pulmonary circulation vascular resistance due to various reasons, which mainly leads to right heart dysfunction and even death, especially in critically ill patients. Although drug interventions have shown some efficacy in improving the hemodynamics of PH patients, the mortality rate remains high. Hence, the identification of new targets and treatment strategies for PH is imperative. Heparanase (HPA) is an enzyme that specifically cleaves the heparan sulfate (HS) side chains in the extracellular matrix, playing critical roles in inflammation and tumorigenesis. Recent studies have indicated a close association between HPA and PH, suggesting HPA as a potential therapeutic target. This review examines the involvement of HPA in PH pathogenesis, including its effects on endothelial cells, inflammation, and coagulation. Furthermore, HPA may serve as a biomarker for diagnosing PH, and the development of HPA inhibitors holds promise as a targeted therapy for PH treatment.
Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms
Authors: Li Mengyuan , Tian Fei , Guo Jinling , Li Xiankuan , Ma Lin , Jiang Miaomiao , Zhao Jing
Published: August 2023

Arthritis is a common degenerative disease of joints, which has become a public health problem affecting human health, but its pathogenesis is complex and cannot be eradicated. Coptis chinensis (CC) has a variety of active ingredients, is a natural antibacterial and anti-inflammatory drug. In which, berberine is its main effective ingredient, and has good therapeutic effects on rheumatoid arthritis (RA), osteoarthritis (OA), gouty arthritis (GA). RA, OA and GA are the three most common types of arthritis, but the relevant pathogenesis is not clear. Therefore, molecular mechanism and prevention and treatment of arthritis are the key issues to be paid attention to in clinical practice. In general, berberine, palmatine, coptisine, jatrorrhizine, magnoflorine and jatrorrhizine hydrochloride in CC play the role in treating arthritis by regulating Wnt1/β-catenin and PI3K/AKT/mTOR signaling pathways. In this review, active ingredients, targets and mechanism of CC in the treatment of arthritis were expounded, and we have further explained the potential role of AHR, CAV1, CRP, CXCL2, IRF1, SPP1, and IL-17 signaling pathway in the treatment of arthritis, and to provide a new idea for the clinical treatment of arthritis by CC.
Ceruloplasmin is associated with the infiltration of immune cells and acts as a prognostic biomarker in patients suffering from glioma
Authors: Jia Miaomiao , Dong Tianyu , Cheng Yangyang , Rong Fanghao , Zhang Jiamin , Lv Wei , Zhen Shuman , Jia Xianxian , Cong Bin , Wu Yuming , Cui Huixian , Hao Peipei
Published: August 2023

Glioma is regarded as a prevalent form of cancer that affects the Central Nervous System (CNS), with an aggressive growth pattern and a low clinical cure rate. Despite the advancement of the treatment strategy of surgical resection, chemoradiotherapy and immunotherapy in the last decade, the clinical outcome is still grim, which is ascribed to the low immunogenicity and tumor microenvironment (TME) of glioma. The multifunctional molecule, called ceruloplasmin (CP) is involved in iron metabolism. Its expression pattern, prognostic significance, and association with the immune cells in gliomas have not been thoroughly investigated. Studies using a variety of databases, including Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and Gliovis, showed that the mRNA and protein expression levels of CP in patients suffering from glioma increased significantly with an increasing glioma grade. Kaplan-Meier (KM) curves and statistical tests highlighted a significant reduction in survival time of patients with elevated CP expression levels. According to Cox regression analysis, CP can be utilized as a stand-alone predictive biomarker in patients suffering from glioma. A significant association between CP expression and numerous immune-related pathways was found after analyzing the data using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA). Tumor Immune Estimation Resource (TIMER) and CIBERSORT analyses indicated a substantial correlation between the CP expression and infiltration of immunocytes in the TME. Additionally, immune checkpoints and CP expression in gliomas showed a favorable correlation. According to these results, patients with glioma have better prognoses and levels of tumor immune cell infiltration when their CP expression is low. As a result, CP could be used as a probable therapeutic target for gliomas and potentially anticipate the effectiveness of immunotherapy.
Microplastic stress in plants: effects on plant growth and their remediations
Authors: Jia Li , Liu Lining , Zhang Yujing , Fu Wenxuan , Liu Xing , Wang Qianqian , Tanveer Mohsin , Huang Liping
Published: August 2023

Microplastic (MP) pollution is becoming a global problem due to the resilience, long-term persistence, and robustness of MPs in different ecosystems. In terrestrial ecosystems, plants are exposed to MP stress, thereby affecting overall plant growth and development. This review article has critically analyzed the effects of MP stress in plants. We found that MP stress-induced reduction in plant physical growth is accompanied by two complementary effects: (i) blockage of pores in seed coat or roots to alter water and nutrient uptake, and (ii) induction of drought due to increased soil cracking effects of MPs. Nonetheless, the reduction in physiological growth under MP stress is accompanied by four complementary effects: (i) excessive production of ROS, (ii) alteration in leaf and root ionome, (iii) impaired hormonal regulation, and (iv) decline in chlorophyll and photosynthesis. Considering that, we suggested that targeting the redox regulatory mechanisms could be beneficial in improving tolerance to MPs in plants; however, antioxidant activities are highly dependent on plant species, plant tissue, MP type, and MP dose. MP stress also indirectly reduces plant growth by altering soil productivity. However, MP-induced negative effects vary due to the presence of different surface functional groups and particle sizes. In the end, we suggested the utilization of agronomic approaches, including the application of growth regulators, biochar, and replacing plastic mulch with crop residues, crop diversification, and biological degradation, to ameliorate the effects of MP stress in plants. The efficiency of these methods is also MP-type-specific and dose-dependent.
The role of stress factors in severity of Cytospora plurivora in greenhouse and field plantings of 13 peach (Prunus persica) cultivars
Authors: Miller Stephan T. , Wright Sean , Stewart Jane E.
Published: August 2023

Understanding the host–pathogen–environmental interactions in a pathosystem is essential for management of diseases and diminished crop yields. Abiotic stressors such as cold damage, water deficit, and high pH soils can be major limiting factors to tree fruit production. Along with decreased yields, these abiotic factors can have direct implications for disease severity within orchards. Cytospora plurivora is a ubiquitous fungal canker pathogen in western Colorado, USA and is a major focus in integrated pest management strategies. This research evaluated the influence of biotic and abiotic stress factors on peach tree health. Thirteen peach cultivars were placed under abiotic stress and inoculated with C. plurivora in greenhouse and field conditions. Under deficit irrigation, C. plurivora infections were significantly larger and more severe in both the greenhouse and field trials when compared with those under the full-irrigation controls. In controlled greenhouse conditions, a positive correlation between lesion size and water potential was evident, but no trend of cultivar tolerance was observed. Furthermore, increase in irrigation water pH, through additions of sodium carbonate and bicarbonate, in the greenhouse trials resulted in decreased leaf water potentials and increased pathogen necrotic tissue volumes (mm3). In field trials, there was no positive relationship between lesion size and water potential; trees with the most negative water potentials had the smallest lesions sizes that did not correspond to cultivar, suggesting that other abiotic or biotic factors may be shielding water stressed trees from increased pathogen aggression. This research highlights the importance of proper irrigation and soil pH management as tools for the management of Cytospora canker in peach orchards.
Genome-wide association study of soybean (Glycine max [L.] Merr.) germplasm for dissecting the quantitative trait nucleotides and candidate genes underlying yield-related traits
Authors: Rani Reena , Raza Ghulam , Ashfaq Hamza , Rizwan Muhammad , Razzaq Muhammad Khuram , Waheed Muhammad Qandeel , Shimelis Hussein , Babar Allah Ditta , Arif Muhammad
Published: August 2023

Soybean (Glycine max [L.] Merr.) is one of the most significant crops in the world in terms of oil and protein. Owing to the rising demand for soybean products, there is an increasing need for improved varieties for more productive farming. However, complex correlation patterns among quantitative traits along with genetic interactions pose a challenge for soybean breeding. Association studies play an important role in the identification of accession with useful alleles by locating genomic sites associated with the phenotype in germplasm collections. In the present study, a genome-wide association study was carried out for seven agronomic and yield-related traits. A field experiment was conducted in 2015/2016 at two locations that include 155 diverse soybean germplasm. These germplasms were genotyped using SoySNP50K Illumina Infinium Bead-Chip. A total of 51 markers were identified for node number, plant height, pods per plant, seeds per plant, seed weight per plant, hundred-grain weight, and total yield using a multi-locus linear mixed model (MLMM) in FarmCPU. Among these significant SNPs, 18 were putative novel QTNs, while 33 co-localized with previously reported QTLs. A total of 2,356 genes were found in 250 kb upstream and downstream of significant SNPs, of which 17 genes were functional and the rest were hypothetical proteins. These 17 candidate genes were located in the region of 14 QTNs, of which ss715580365, ss715608427, ss715632502, and ss715620131 are novel QTNs for PH, PPP, SDPP, and TY respectively. Four candidate genes, Glyma.01g199200, Glyma.10g065700, Glyma.18g297900, and Glyma.14g009900, were identified in the vicinity of these novel QTNs, which encode lsd one like 1, Ergosterol biosynthesis ERG4/ERG24 family, HEAT repeat-containing protein, and RbcX2, respectively. Although further experimental validation of these candidate genes is required, several appear to be involved in growth and developmental processes related to the respective agronomic traits when compared with their homologs in Arabidopsis thaliana. This study supports the usefulness of association studies and provides valuable data for functional markers and investigating candidate genes within a diverse germplasm collection in future breeding programs.
Facilitating collaborative professional development among instrumental and vocal teachers: A qualitative study with an Austrian Music School
Authors: Kruse-Weber Silke , Bucura Elizabeth , Tumler Margareth
Published: August 2023

This case study provides an in-depth investigation in a professional development project about facilitating collaborative reflection. This was led by a research team from the university with 13 instrumental music teachers from one music school in Styria (Austria) during 2019–2021 (including the initial COVID-19 pandemic). Research questions considered (1) the participants’ descriptions of the collaborative professional development, (2) participants’ uses of reflection tools and indications of their identification with workshop interventions, as well as factors responsible for the outcomes from the reflection tools; and (3) ways participants’ thinking and attitudes may have developed through the workshops, how they defined themselves as a group (if they did), and how they might have gained trust in one another. Inspired by the design-based research approach, practitioners and researchers worked closely together to enhance teaching and learning implementing interventions with collaborative reflections tools. While the first phase (11 workshops) was primarily led by the project-team, the second phase (7 workshops) was participant-led. Data included focus groups and discussion transcriptions from 18 workshops. The impetus of the study included the role of the director and the participants dealing with the interventions, and finally the participants’ descriptions of their experiences in the professionalization process. Literature included collaborative professional development, community of practice, learning communities, self-determined learning, reflective practice, and ethical considerations. Data were analyzed based on thematic analysis and gave rise to five following themes: forming group cohesion, inspiring and appreciating collaboration, bridging theory and practice, identifying deeper thinking and teachers as learners, addressing challenges and potentials during the COVID-19 pandemic, and finally finding the music school’s own identity and sense of importance. Findings highlight the importance of establishing meaningful collaborative reflection through appreciative communication and an atmosphere of trust and respect. To be able to make change in and with an institution, leadership members must be engaged as collaborative stakeholders on an eye-level; collaborative professional development can be used as a resource toward rethinking and reworking the identity of one’s music school and of teaching and learning. Institutions should provide space and continuity for such development. Finally, the study highlights that a collaborative reflective approach can contribute to professional and social growth.
Enriched sensory feedback delivered during a voluntary action boosts subjective time compression
Authors: Ueda Sayako , Shimoda Shingo
Published: August 2023

Introduction

The subjective experience of time can be influenced by various factors including voluntary actions. In our previous study, we found that the subjective time experience of an action outcome can be compressed when an individual performs a continuous action compared to a single action, suggesting that the sense of agency (SoA), the feeling of control over one’s own action outcomes, contributes to the subjective time compression. We hypothesized that enhancing SoA by providing sensory feedback to participants would further compress the subjective time experience.

Methods

To test the hypothesis, we used a temporal reproduction task where participants reproduced the duration of a previously exposed auditory stimulus by performing different voluntary actions: a combination of single actions with single auditory feedback, continuous action with single auditory feedback, or continuous action with multiple auditory feedback.

Results

The results showed that the continuous action conditions, regardless of the type of auditory feedback, led to a compression of the subjective time experience of the reproduced tone, whereas the single action condition did not. Furthermore, a greater degree of subjective time compression during continuous action and a stronger SoA were revealed when enriched with multiple auditory feedback.

Discussion

These results indicate that enriching auditory feedback can increase subjective time compression during voluntary action, which in turn enhances SoA over action outcomes. This suggests the potential for developing new techniques to artificially compress the subjective time experience of daily events.
Intergenerational trauma and war-induced PTSD in Kosovo: insights from the Albanian ethnic group
Authors: Hyseni Duraku Zamira , Jahiu Genta , Geci Donjeta
Published: August 2023

Introduction

War has profound and deep-rooted ramifications for individuals and societies. War-induced post-traumatic stress disorder (PTSD) is highly prevalent in Kosovo. This study aimed to obtain insights into the prevalence of perceived PTSD symptoms and their relation to the traumatic experiences of two generations: parents (survivors of the Kosovo War) and youth (children born after the Kosovo War), with an emphasis on the Albanian ethnic group. These experiences were then compared to understand intergenerational trauma. The study also aimed to identify the factors affecting PTSD prevalence, the role of social support, and the participants’ experience with mental health services.

Method

A total of 237 Kosovar Albanians (121 parents, 116 youth) from all seven districts of Kosovo were included in this study. Study variables were measured using the PTSD Checklist, the Life Events Checklist, Criterion A, and the Multidimensional Scale of Perceived Social Support.

Results

The results revealed that the youth had significantly higher levels of perceived PTSD symptoms and lower levels of perceived support than their parents. Youth whose parents had PTSD were more prone to experiencing PTSD symptoms than those whose parents did not have PTSD. These youth also experienced significantly more traumatic situations, such as exposure to sudden violent death or accidental death, assault with a weapon, sexual assault, and captivity. Participants with perceived PTSD and lower perceived social support needed mental health interventions significantly more than those without PTSD symptoms.

Discussion

The findings emphasize the importance of addressing the intergenerational nature of PTSD and identifying factors affecting its prevalence, including social support and access to mental health services. The study underscores the need for a comprehensive approach to examine the complex and diverse nature of PTSD and its impact on individuals, families, and communities, especially in conflict-prone or conflict-affected societies.
Type 1 diabetes in North East England and North Cumbria: patterns and time trends in 0–14-year-olds from 2012 to 2020
Authors: Hayes Louise , Cheetham Tim , Muirhead Colin , Hopper Neil , Reid Judith , Lamb William , Foster Jenny , McNally Richard J. Q.
Published: August 2023

Introduction

It is important to understand patterns in the epidemiology of type 1 diabetes because they may provide insight into its etiology. We examined the incidence of type 1 diabetes in children aged 0–14 years, and patient demographics and clinical parameters at presentation, over the period 2012–2020 using the North East and North Cumbria Young Persons diabetes register.

Methods

Patients up to the age of 14 years with type 1 diabetes, and their families- managed in a total of 18 young persons diabetes clinics—were approached in person at the time of clinic appointments or in the days following diagnosis and they consented to their data being included in the register. Data were submitted regionally to a central unit. Descriptive statistics including crude and age-specific incidence rates were calculated. Temporal trends were analyzed using Joinpoint regression. Comparisons in incidence rates were made between age, sex and areas of higher and lower affluence as measured by the Index of Multiple Deprivation (IMD).

Results

A total of 943 cases were recorded between January 2012 and December 2020. Median age at diagnosis was 8.8 years (Q1: 5.3, Q3: 11.7). There were more males than females (54% male). The median HbA1c at diagnosis was 100 mmoL/L (IQR: 39) and over one third (35%) were in ketoacidosis (pH < 7.3). Crude incidence decreased from 25.5 (95% confidence interval [CI] 20.9, 29.9) in 2012 to 16.6 (95% CI: 13.0, 20.2) per 100,000 in 2020 (5.1% per annum, 95% CI 1.1, 8.8%). During the period of the study there was no evidence of any trends in median age, HbA1c, BMI or birthweight (p = 0.18, 0.80, 0.69, 0.32) at diagnosis. Higher rates were observed in males aged 10–14 years, but similar rates were found for both sexes aged 0–9 years and there was no difference between areas of higher or lower deprivation (p = 0.22).

Conclusion

The incidence of diabetes in the young may be falling in the North East of England and North Cumbria. The reasons are unclear as there were no associations identified between levels of deprivation or anthropometric measurements. Potential mechanisms include alterations in socioeconomic background or growth pattern. Further research is needed to understand the reasons behind this finding.
Cytokine associated with severity of depressive symptoms in female nurses in Korea
Authors: Kim Yoonjoo , Pang Yanghee , Park Hyunki , Kim Oksoo , Lee Hyangkyu
Published: August 2023

Background

Depression has been associated with the risk of developing physical illnesses and diseases. Inflammatory hypotheses of immunoactive and dysregulated cytokine production have been proposed to describe this association; however, data pertaining to the high prevalence of depression among nurses are limited.

Objective

This study aimed to use a comprehensive immune-profiling approach to determine whether an abnormal profile of circulating cytokines could be identified in nurses with self-reported depression and whether this profile is associated with the severity of depression.

Methods

We investigated a cohort of 157 female nurses in Korea. The self-report Patient Health Questionnaire was used to measure the depression levels of nurses. In addition, peripheral blood samples were collected and used to measure the cytokine profile using the Luminex multiplexing system. Generalized gamma regression analyses were conducted to evaluate the association between cytokine and depressive symptoms.

Results

Regarding severity of depressive symptoms, 28.0% of nurses had moderately severe depression while 9.6% had severe depression. Moderately-severe depressive symptoms in nurses were associated with elevated levels of interleukin-6 (B = 0.460, p = 0.003), interleukin-8 (B = 0.273, p = 0.001), and interleukin-18 (B = 0.236, p = 0.023), whereas interferon-gamma levels (B = −0.585, p = 0.003) showed the opposite profile. Participants with severe depressive symptoms presented decreased interferon-gamma levels (B = −1.254, p < 0.001).

Conclusion

This study demonstrated that proinflammatory cytokines were associated with depression among nurses. This calls for early detection and intervention, considering the mechanisms linking depression to physical illness and disease.
Burnout, self-rated general health and life satisfaction among teachers and other academic occupational groups
Authors: Beutel Till , Koestner Clemens , Wild Philipp S. , Münzel Thomas , Beutel Manfred E. , Lackner Karl J. , Pfeiffer Norbert , Nübling Matthias , Becker Jan , Letzel Stephan
Published: August 2023

Introduction

Teachers work in a job with specific demands that can strain individual coping capabilities and can pose a risk for the development of psychological problems. Prior studies showed that teachers – in comparison with other occupational groups – had high risks of job-related psychological exhaustion. In our study we compared teachers and other occupational groups on burnout, general life satisfaction and self-rated general health. In addition, we analyzed if sociodemographic and job-related factors were relevant predictors of these outcomes.

Methods

We analyzed data from a total of 1,500 subjects arising from the Gutenberg Health Study. Binary logistic regression models and descriptive statistics were calculated to determine potential differences between the occupational group membership and the predictive values of sociodemographic and job-related variables.

Results

The occupational groups did not differ significantly in terms of burnout, self-rated general health and satisfaction with life. Logistic regression models showed which sociodemographic and job-related variables were associated with the outcomes. Female sex, part-time employment as well as work-privacy conflicts showed particular predictive relevance.

Discussion

Job-related interventions for teachers should aim at specific strains, e.g., arising out of work-privacy conflicts where interventions should focus on support of female teachers.
Metagenomic next-generation sequencing for Mycobacterium tuberculosis complex detection: a meta-analysis
Authors: Li Yulian , Bian Wentao , Wu Shiping , Zhang Jie , Li Dan
Published: August 2023

Objective

Metagenomic next-generation sequencing (mNGS) has been gradually applied to the diagnosis of tuberculosis (TB) due to its rapid and highly sensitive characteristics. Despite numerous studies on this subject, their results vary significantly. Thus, the current meta-analysis was performed to assess the performance of the mNGS on tuberculosis.

Methods

PubMed, Embase, Web of Science, and The Cochrane Library were searched up to June 21, 2023. Studies utilizing the mNGS for tuberculosis detection were included. The risk of bias was assessed by QUADAS-2, and a meta-analysis was performed with STATA14.0 software.

Results

Seventeen studies comprising 3,205 specimens were included. The combined sensitivity and specificity of mNGS for clinical specimens were 0.69[0.58–0.79] and 1.00[0.99–1.00], respectively. Subgroup analysis identified sequencing platform, diagnostic criteria, study type, sample size, and sample types as potential sources of heterogeneity. Cerebrospinal Fluid (CSF) has a lower sensitivity of 0.58 (0.39–0.75). In a population with a 10% prevalence rate, the accuracy of sensitivity reached 94%.

Conclusion

Metagenomic next-generation sequencing technology exhibits high sensitivity and speed in diagnosing Mycobacterium tuberculosis. Its application in mono and mixed infections peoples shows promise, and mNGS is likely to be increasingly used to address challenges posed by Mycobacterium tuberculosis complexes in the future.
Coordinating culture change across the research landscape
Authors: McIntosh Leslie D. , Hudson Vitale Cynthia
Published: August 2023

Scientific integrity necessitates applying scientific methods properly, collecting and analyzing data appropriately, protecting human subjects rightly, performing studies rigorously, and communicating findings transparently. But who is responsible for upholding research integrity, mitigating misinformation, and increasing trust in science beyond individual researchers? We posit that supporting the scientific reputation requires a coordinated approach across all stakeholders: funding agencies, publishers, scholarly societies, research institutions, and journalists and media, and policy-makers.
Perspectives and practices of nutritionists on dietary supplements for elite soccer teams: a cross-sectional survey study
Authors: Abreu Rodrigo , Oliveira Catarina B. , Brito João , Teixeira Vitor H.
Published: August 2023

Introduction and objectives

Dietary supplements are part of the nutritional strategies frequently applied in sports performance support. With growing research on this subject and high demand from athletes, nutritionists need to keep up to date with the latest evidence and utility of dietary supplements, particularly in real-world contexts. As information about the use of dietary supplements among elite soccer players is still scarce, this work aimed to know how nutritionists working with elite soccer teams perceive and use these substances in their daily practice.

Methods

A questionnaire previously used to describe nutritionists’ beliefs and attitudes regarding the use of dietary supplements in a clinical context was adapted for this study. The online questionnaire was addressed to nutritionists working with elite soccer teams from six European Leagues and Brazil, between November 2022 and February 2023.

Results

Overall, the participants considered themselves well-trained (76.9%), knowledgeable (95.4%), and interested in dietary supplements (95.4%). The majority (70.8%) of the participants agreed or strongly agreed to recommend dietary supplements to soccer players. Personal usage of dietary supplements was associated with recommending supplements (p < 0.001), but no relationships were found with years of experience and academic level.

Discussion

Nutritionists working with elite soccer players consider the use of dietary supplements for performance-enhancement purposes and not only to compensate for nutritional deficits, which might contribute to their higher interest, training and perceived knowledge about this topic. Participants recognize players’ interest in dietary supplements, and are mindful of the safety and efficacy of these products. The present study suggests that nutritionists working with elite soccer teams are among the highest prescribers of dietary supplements, although personal usage is lower than that of nutritionists working in a clinical context.
ICD-Based Cause of Death Statistics Fail to Provide Reliable Data for Medical Aid in Dying
Authors: Güth Uwe , Junker Christoph , Schafroth Marion , McMillan Shaun , Schneeberger Andres R. , Elfgen Constanze , Battegay Edouard , Weitkunat Rolf
Published: August 2023

Objectives: To evaluate the most recent developments of medical aid in dying (MAID) in Switzerland and to test the reliability of reporting this phenomenon in cause of death statistics.

Methods: By reviewing the MAID cases between 2018 and 2020, we compared the diseases and conditions underlying MAID reported by the ICD-based statistics provided by the Swiss Federal Statistical Office (FSO, n = 3,623) and those provided by the largest right-to-die organization EXIT (n = 2,680).

Results: EXIT reported the motivations underlying the desire for death in a mixture of disease-specific and symptom-oriented categories; the latter including, for example, multimorbidity (26% of cases), and chronic pain (8%). Symptom-oriented categories were not included in the ICD-based FSO statistics. This led to the fact that the distribution of the diseases/conditions underlying MAID differed in 30%–40% of cases between both statistics.

Conclusion: In order to reliably follow developments and trends in MAID, the diseases/conditions underlying the wish to die must be accurately recorded. Current methods of data collection using the ICD classification do not capture this information thoroughly (“MAID gap”). Newly created ICD codes for MAID must include both disease-specific and symptom-oriented categories.
Hour-by-Hour Prediction Model of Air Pollutant Concentration Based on EIDW-Informer—A Case Study of Taiyuan
Authors: Lai Kefu , Xu Huahu , Sheng Jun , Huang Yuzhe
Published: August 2023

Prediction of air pollutant concentrations is currently one of the most important methods for the prevention and control of urban air pollution in most countries, and accurate and timely prediction of pollutant concentrations is of great significance for urban pollution control. Using Taiyuan, China, as a case study, this study examines how to predict hourly air pollutant concentrations over longer periods of time while ensuring their accuracy. In this paper, an air pollutant concentration prediction method based on improved inverse distance interpolation and Informer model (EIDW-Informer), and hour-by-hour prediction of PM2.5, NO2, and O3 concentrations in Taiyuan, China is carried out. In this study, historical data from seven environmental monitoring stations in Taiyuan City were used to build multidimensional environmental vectors and calculate the similarity between sample points. Then, the missing values in the dataset were interpolated according to the similarity and distance weights, and the long series prediction was performed by Informer. The experimental results show that the EIDW-Informer method has advantages in hour-by-hour prediction compared to LSTM, CNN-LSTM, and Attention-LSTM models, which improves by 20%, 27%, and 43% on 1 h, 8 h, and 72 h time scales, respectively.
Sturm-Liouville Problem with Mixed Boundary Conditions for a Differential Equation with a Fractional Derivative and Its Application in Viscoelasticity Models
Authors: Kiryanova Ludmila , Matseevich Tatiana
Published: August 2023

In this study, we obtained a system of eigenfunctions and eigenvalues for the mixed homogeneous Sturm-Liouville problem of a second-order differential equation containing a fractional derivative operator. The fractional differentiation operator was considered according to two definitions: Gerasimov-Caputo and Riemann-Liouville-Visualizations of the system of eigenfunctions, the biorthogonal system, and the distribution of eigenvalues on the real axis were presented. The numerical behavior of eigenvalues was studied depending on the order of the fractional derivative for both definitions of the fractional derivative.
Stress Factors as Possible Regulators of Pluripotent Stem Cell Survival and Differentiation
Authors: Darwish Toqa , Swaidan Nuha Taysir , Emara Mohamed M.
Published: August 2023

In recent years, extensive research efforts have been directed toward pluripotent stem cells, primarily due to their remarkable capacity for pluripotency. This unique attribute empowers these cells to undergo self-renewal and differentiate into various cell types originating from the ectoderm, mesoderm, and endoderm germ layers. The delicate balance and precise regulation of self-renewal and differentiation are essential for the survival and functionality of these cells. Notably, exposure to specific environmental stressors can activate numerous transcription factors, initiating a diverse array of stress response pathways. These pathways play pivotal roles in regulating gene expression and protein synthesis, ultimately aiming to preserve cell survival and maintain cellular functions. Reactive oxygen species, heat shock, hypoxia, osmotic stress, DNA damage, endoplasmic reticulum stress, and mechanical stress are among the examples of such stressors. In this review, we comprehensively discuss the impact of environmental stressors on the growth of embryonic cells. Furthermore, we provide a summary of the distinct stress response pathways triggered when pluripotent stem cells are exposed to different environmental stressors. Additionally, we highlight recent discoveries regarding the role of such stressors in the generation, differentiation, and self-renewal of induced pluripotent stem cells.
Shoulder Pain Biomechanics, Rehabilitation and Prevention in Wheelchair Basketball Players: A Narrative Review
Authors: Farì Giacomo , Latino Francesca , Tafuri Francesco , Dell’Anna Laura , Raele Maria Vittoria , Fai Annatonia , De Serio Carlo , Intonti Giorgia , De Salvo Anna Lisa , Ricci Vincenzo , Saraiello Emma , Bonavolontà Valerio , Bernetti Andrea , Fiore Silvia , Megna Marisa , Ranieri Maurizio
Published: August 2023

Wheelchair basketball (WB) is an increasingly popular sport that guarantees numerous health benefits for people with disabilities who regularly practice it, such as an improved quality of life and psychophysical well-being. However, WB is a contact and high-stress sport, which exposes players to frequent overloads and injuries, mainly affecting the upper limbs. Therefore, shoulder pain (SP) is the most common musculoskeletal disorder among WB players, forcing them to suspend or abandon this sport activity. This narrative review aims to summarize all the known literature on this topic and to be a starting point for further research. Firstly, it explores the biomechanical causes that lead to SP and the underlying diseases, among which the most recurrent are rotator cuff tendinopathies. Furthermore, this overview deepens the most effective and specific rehabilitation programs for SP in WB players and it emphasizes the need for further studies to trial new rehabilitative protocols using novel technologies to make them faster and more personalized. In this regard, the general recommendation still remains to perform a combination of exercises such as strengthening, endurance and stretching exercises of various durations and intensities. To conclude, the most important prevention strategies are described, underlining the need for constant sport-specific training led by qualified personnel and suggesting some insights on possible new research aimed at improving wheelchair ergonomics, stressing the importance of a multidisciplinary team fully dedicated to the individual athlete.
Characterization of Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-Antigens in Human Sera
Authors: Aruta Maria Grazia , Lari Elisa , De Simone Daniele , Semplici Bianca , Semplici Claudia , Dale Helen , Chirwa Esmelda , Kadwala Innocent , Mbewe Maurice , Banda Happy , Iturriza-Gomara Miren , Gordon Melita , Nyirenda Tonney , Piu Pietro , Pizza Mariagrazia , Berlanda Scorza Francesco , Grappi Silvia , Canals Rocío , Rossi Omar ,   on behalf of the Vacc-iNTS Consortium Collaborators
Published: August 2023

Nontyphoidal Salmonella (NTS) is a leading cause of morbidity and mortality caused by enteric pathogens worldwide in both children and adults, and vaccines are not yet available. The measurement of antigen-specific antibodies in the sera of vaccinated or convalescent individuals is crucial to understand the incidence of disease and the immunogenicity of vaccine candidates. A solid and standardized assay used to determine the level of specific anti-antigens IgG is therefore of paramount importance. In this work, we presented the characterization of a customized enzyme-linked immunosorbent assay (ELISA) with continuous readouts and a standardized definition of EU/mL. We assessed various performance parameters: standard curve accuracy, dilutional linearity, intermediate precision, specificity, limits of blanks, and quantification. The simplicity of the assay, its high sensitivity and specificity coupled with its low cost and the use of basic consumables and instruments without the need of high automation makes it suitable for transfer and application to different laboratories, including resource-limiting settings where the disease is endemic. This ELISA is, therefore, fit for purpose to be used for quantification of antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-antigens in human samples, both for vaccine clinical trials and large sero-epidemiological studies.
Locoregional Treatment for Early-Stage Breast Cancer: Current Status and Future Perspectives
Authors: Lavasani Sayeh , Healy Erin , Kansal Kari
Published: August 2023

Background: The locoregional recurrence of breast cancer has been reduced due to the multidisciplinary approach of breast surgery, systemic therapy and radiation. Early detection and better surgical techniques contribute to an improvement in breast cancer outcomes. Purpose of Review: The purpose of this review is to have an overview and summary of the current evidence behind the current approaches to the locoregional treatment of breast cancer and to discuss its future direction. Summary: With improved surgical techniques and the use of a more effective neoadjuvant systemic therapy, including checkpoint inhibitors and dual HER2-directed therapies that lead to a higher frequency of pathologic complete responses and advances in adjuvant radiation therapy, breast cancer patients are experiencing better locoregional control and reduced local and systemic recurrence. De-escalation in surgery has not only improved the quality of life in the majority of breast cancer patients, but also maintained the low risk of recurrence. There are ongoing clinical trials to optimize radiation therapy in breast cancer. More modern radiation technologies are evolving to improve the patient outcome and reduce radiation toxicities.
Seasonal Variations in Plant Species Diversity and Phylogenetic Diversity in Abandoned Farmland of China’s Huang–Huai Plain
Authors: Yan Peisen , Lu Xunling , Li Wanying , Zhang Jian , Li Peikun , Li Yan , Wang Kaiyue , Ding Shengyan
Published: August 2023

Amidst urbanization and industrialization in China, abandoned farmland plays a crucial role in safeguarding plant diversity within agricultural landscapes. This study aimed to examine the patterns of seasonal variation in plant diversity within abandoned farmland habitats in the Huang–Huai Plain region. Nonparametric tests were employed to analyze plant species diversity and phylogenetic diversity across seasons. Redundancy analysis and linear regression were conducted to examine the associations between plant species composition, species diversity, phylogenetic diversity, and soil environmental factors. Our results showed that plant species diversity, richness, and phylogenetic diversity were highest in spring, followed by summer, and lowest in autumn. The phylogenetic structure of plant communities demonstrated a tendency to diverge in spring, become random in summer, and cluster in autumn. Soil available potassium and soil organic matter emerged as important factors influencing plant species composition. The content of soil organic matter and ammonium nitrogen level exhibited a significantly positive correlation with the species diversity and phylogenetic diversity of plants. This study underscores the significance of considering seasonal and temporal scales when investigating plant diversity and provides a theoretical basis for biodiversity conservation in agricultural landscapes.
University Experience during the First Two Waves of COVID-19: Students’ Experiences and Psychological Wellbeing
Authors: La Rosa Valentina Lucia , Commodari Elena
Published: August 2023

Background: This study aimed to analyze Italian university students’ psychological needs, learning experiences, and wellbeing during the first two waves of the COVID-19 pandemic. Methods: The study was carried out during the first wave of the pandemic, and then during the second. A total of 1230 university students (654 in stage 1 and 576 in stage 2) completed a battery of validated questionnaires exploring students’ experiences in distance learning, perceived health risks related to COVID-19, and psychological wellbeing. Results: MANOVA showed a significant main effect of the pandemic stage on students’ learning experience and wellbeing. In particular, students were more distracted and concerned about their academic careers during the second phase of the pandemic than in the first. Furthermore, the pandemic stage also significantly affected health risk perceptions and fear of COVID-19, which were higher during the second wave of the pandemic. Female gender and concern for a university career were significant risk factors for high levels of negative affectivity and low levels of positive affectivity during the pandemic. In contrast, adherence to COVID-19 restriction measures and good family support were protective factors. Conclusions: It is essential to promote adequate university psychological services to support university students who have experienced the adverse psychological effects of the pandemic and enhance the resilience factors needed to improve their wellbeing in the post-pandemic period.