bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Morphology on the Bulk Density of Instant Whole Milk Powder
Authors: Ding Haohan , Li Bing , Boiarkina Irina , Wilson David I. , Yu Wei , Young Brent R.
Published: July 2020

The chemical and physical properties of instant whole milk powder (IWMP), such as morphology, protein content, and particle size, can affect its functionality and performance. Bulk density, which directly determines the packing cost and transportation cost of milk powder, is one of the most important functional properties of IWMP, and it is mainly affected by physical properties, e.g., morphology and particle size. This work quantified the relationship between morphology and bulk density of IWMP and developed a predictive model of bulk density for IWMP. To obtain milk powder samples with different particle size fractions, IWMP samples of four different brands were sieved into three different particle size range groups, before using the simplex-centroid design (SCD) method to remix the milk powder samples. The bulk densities of these remixed milk powder samples were then measured by tap testing, and the particles’ shape factors were extracted by light microscopy and image processing. The number of variables was decreased by principal component analysis and partial least squares models and artificial neural network models were built to predict the bulk density of IWMP. It was found that different brands of IWMP have different morphology, and the bulk density trends versus the shape factor changes were similar for the different particle size range groups. Finally, prediction models for bulk density were developed by using the shape factors and particle size range fractions of the IWMP samples. The good results of these models proved that predicting the bulk density of IWMP by using shape factors and particle size range fractions is achievable and could be used as a model for online model-based process monitoring.
A Method for the Rapid Measurement of Alkylresorcinols in Flour, Bread and Related Products Based on 1H qNMR
Authors: Tsirivakou Athina , Melliou Eleni , Magiatis Prokopios
Published: July 2020

The main objectives of the current work were to investigate differences among flours from traditionally preserved Greek varieties of cereals, and especially those of wheat, and in parallel, to correlate those potential differences with the presence of bioactive natural ingredients. In this context, we developed a new, fast, and simple method for the measurement of total 5-alkylresorcinols in cereals and related foods by qNMR. Several types of flour (white or whole-grain) coming from wheat, i.e., Triticum dicoccum, T. monococcum, T. aestivum, T. durum and T. turgidum, corn, barley, rye and oat from a certified producer in Greece were used either as raw materials or for the production of bread, pasta and flakes. A small portion of the flour or the corresponding product was extracted with DMSO-d6. The liquid part was directly analyzed by NMR (400 MHz). The simplicity of the NMR spectrum of the total extract and the lack of overlapping peaks permitted the development of a high throughput quantitative method for the measurement of total bioactive alkylresorcinols in less than 15 min. Grains, whole grain flours and breads from old varieties of T. dicoccum and T.monococcum showed high contents of alkylresorcinols (455–1148 mg/Kg), while the same compounds were completely absent from white flour and the corresponding bread. The term high-phenolic flour is proposed to distinguish among flour types.
Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic
Authors: Mlejnkova Hana , Sovova Katerina , Vasickova Petra , Ocenaskova Vera , Jasikova Lucie , Juranova Eva
Published: July 2020

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.
Lack of Correlation between Accelerometers and Heart-Rate Monitorization during Exercise Session in Older Adults
Authors: Carbonell-Hernández Laura , Pastor Diego , Jiménez-Loaisa Alejandro , Ballester-Ferrer Juan Arturo , Montero-Carretero Carlos , Cervelló Eduardo
Published: July 2020

Aging is increasing worldwide; hence, aging-related health is also more relevant. Well-programmed physical exercise is now an indispensable tool to achieve active aging and preserve older people’s health. Such “well-programmed” exercise requires efficient and useful tools to measure the activity. The objective of this study is to evaluate the effectiveness of accelerometers to estimate two different intensities of physical exercise in older people. Thirty-eight subjects (64.5 ± 5.3 years) were measured during two different sessions of physical exercise: one moderate in intensity, the other of low intensity. Heart rate and accelerometry were recorded and analyzed. The results showed that the two variables in the physical exercise sessions were not highly correlated, and that accelerometry did not seem useful to assess low-intensity sessions not based on walking.
Selecting Appropriate Words for Naming the Rows and Columns of Risk Assessment Matrices
Authors: Jensen Roger C. , Hansen Haley
Published: July 2020

The risk management systems used in occupational safety and health typically assess the risk of identified hazards using a tabular format commonly called a risk assessment matrix. Typically, columns are named with words indicating severity, and rows are named with words indicating likelihood or probability. Some risk assessment matrices use words reflecting the extent of exposure to a hazard. This project was undertaken with the aim of helping the designers of risk assessment matrices select appropriate names for the rows and columns. A survey of undergraduate students studying engineering or occupational safety and health obtained ratings of 16 English language words and phrases for each of the three factors. Analyses of 84 completed surveys included comparing average ratings on a 100-point scale. Using the averages, appropriately spaced sets of words and phrases were identified for naming the row and column categories. Based on results, the authors recommend word sets of three, four, and five for severity; three, four, five, and six for likelihood; and two and three for extent of exposure. The study methodology may be useful for future research, and the resulting word sets and numerical ratings may be helpful when creating a new, or reassessing an established, risk assessment matrix.
Comparing Fully Deep Convolutional Neural Networks for Land Cover Classification with High-Spatial-Resolution Gaofen-2 Images
Authors: Han Zemin , Dian Yuanyong , Xia Hao , Zhou Jingjing , Jian Yongfeng , Yao Chonghuai , Wang Xiong , Li Yuan
Published: July 2020

Land cover is an important variable of the terrestrial ecosystem that provides information for natural resources management, urban sprawl detection, and environment research. To classify land cover with high-spatial-resolution multispectral remote sensing imagery is a difficult problem due to heterogeneous spectral values of the same object on the ground. Fully convolutional networks (FCNs) are a state-of-the-art method that has been increasingly used in image segmentation and classification. However, a systematic quantitative comparison of FCNs on high-spatial-multispectral remote imagery was not yet performed. In this paper, we adopted the three FCNs (FCN-8s, Segnet, and Unet) for Gaofen-2 (GF2) satellite imagery classification. Two scenes of GF2 with a total of 3329 polygon samples were used in the study area and a systematic quantitative comparison of FCNs was conducted with red, green, blue (RGB) and RGB+near infrared (NIR) inputs for GF2 satellite imagery. The results showed that: (1) The FCN methods perform well in land cover classification with GF2 imagery, and yet, different FCNs architectures exhibited different results in mapping accuracy. The FCN-8s model performed best among the Segnet and Unet architectures due to the multiscale feature channels in the upsampling stage. Averaged across the models, the overall accuracy (OA) and Kappa coefficient (Kappa) were 5% and 0.06 higher, respectively, in FCN-8s when compared with the other two models. (2) High-spatial-resolution remote sensing imagery with RGB+NIR bands performed better than RGB input at mapping land cover, and yet the advantage was limited; the OA and Kappa only increased an average of 0.4% and 0.01 in the RGB+NIR bands. (3) The GF2 imagery provided an encouraging result in estimating land cover based on the FCN-8s method, which can be exploited for large-scale land cover mapping in the future.
Evaluation of Spatial Resilience of Highway Networks in Response to Adverse Weather Conditions
Authors: Han Fei , Zhang Su
Published: July 2020

Adverse weather poses a significant threat to the serviceability of highway infrastructure, as it causes more frequent and severe crash incidents. This study focuses on evaluating the resilience of highway networks by examining the crash-induced safety impact in response to extreme weather events. Unlike traditional service drop-based methods for resilience evaluation, this study endeavors to evaluate highway resilience in a spatial context. Three spatial metrics, including K-nearest neighbors, proximity to highways, and Kernel density hot spot, are introduced and employed to compare and analyze the spatial patterns (magnitude and distribution) of crashes in pre- and post-weather conditions. An illustrative example is also provided to showcase the applications of the proposed spatial resilience metrics for two study areas in the State of Illinois, U.S. The contribution of this study is to provide transportation practitioners with a tool to evaluate highway spatial resilience both visually and quantitatively, and ultimately improve highway safety and operation.
Parthenolide Has Negative Effects on In Vitro Enhanced Osteogenic Phenotypes by Inflammatory Cytokine TNF-α via Inhibiting JNK Signaling
Authors: Park Jin-Ho , Kang Young-Hoon , Hwang Sun-Chul , Oh Se Heang , Byun June-Ho
Published: July 2020

Nuclear factor kappa B (NF-κB) regulates inflammatory gene expression and represents a likely target for novel disease treatment approaches, including skeletal disorders. Several plant-derived sesquiterpene lactones can inhibit the activation of NF-κB. Parthenolide (PTL) is an abundant sesquiterpene lactone, found in Mexican Indian Asteraceae family plants, with reported anti-inflammatory activity, through the inhibition of a common step in the NF-κB activation pathway. This study examined the effects of PTL on the enhanced, in vitro, osteogenic phenotypes of human periosteum-derived cells (hPDCs), mediated by the inflammatory cytokine tumor necrosis factor (TNF)-α. PTL had no significant effects on hPDC viability or osteoblastic activities, whereas TNF-α had positive effects on the in vitro osteoblastic differentiation of hPDCs. c-Jun N-terminal kinase (JNK) signaling played an important role in the enhanced osteoblastic differentiation of TNF-α-treated hPDCs. Treatment with 1 µM PTL did not affect TNF-α-treated hPDCs; however, 5 and 10 µM PTL treatment decreased the histochemical detection and activity of alkaline phosphatase (ALP), alizarin red-positive mineralization, and the expression of ALP and osteocalcin mRNA. JNK phosphorylation decreased significantly in TNF-α-treated hPDCs pretreated with PTL. These results suggested that PTL exerts negative effects on the increased osteoblastic differentiation of TNF-α-treated hPDCs by inhibiting JNK signaling.
Expanding Role of Dopaminergic Inhibition in Hypercapnic Responses of Cultured Rat Carotid Body Cells: Involvement of Type II Glial Cells
Authors: Leonard Erin M. , Nurse Colin A.
Published: July 2020

Dopamine (DA) is a well-studied neurochemical in the mammalian carotid body (CB), a chemosensory organ involved in O2 and CO2/H+ homeostasis. DA released from receptor (type I) cells during chemostimulation is predominantly inhibitory, acting via pre- and post-synaptic dopamine D2 receptors (D2R) on type I cells and afferent (petrosal) terminals respectively. By contrast, co-released ATP is excitatory at postsynaptic P2X2/3R, though paracrine P2Y2R activation of neighboring glial-like type II cells may boost further ATP release. Here, we tested the hypothesis that DA may also inhibit type II cell function. When applied alone, DA (10 μM) had negligible effects on basal [Ca2+]i in isolated rat type II cells. However, DA strongly inhibited [Ca2+]i elevations (Δ[Ca2+]i) evoked by the P2Y2R agonist UTP (100 μM), an effect opposed by the D2/3R antagonist, sulpiride (1–10 μM). As expected, acute hypercapnia (10% CO2; pH 7.4), or high K+ (30 mM) caused Δ[Ca2+]i in type I cells. However, these stimuli sometimes triggered a secondary, delayed Δ[Ca2+]i in nearby type II cells, attributable to crosstalk involving ATP-P2Y2R interactions. Interestingly sulpiride, or DA store-depletion using reserpine, potentiated both the frequency and magnitude of the secondary Δ[Ca2+]i in type II cells. In functional CB-petrosal neuron cocultures, sulpiride potentiated hypercapnia-induced Δ[Ca2+]i in type I cells, type II cells, and petrosal neurons. Moreover, stimulation of type II cells with UTP could directly evoke Δ[Ca2+]i in nearby petrosal neurons. Thus, dopaminergic inhibition of purinergic signalling in type II cells may help control the integrated sensory output of the CB during hypercapnia.
Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels
Authors: Webster Jack M. , Darling April L. , Sanders Taylor A. , Blazier Danielle M. , Vidal-Aguiar Yamile , Beaulieu-Abdelahad David , Plemmons Drew G. , Hill Shannon E. , Uversky Vladimir N. , Bickford Paula C. , Dickey Chad A. , Blair Laura J.
Published: July 2020

Misfolding, aggregation and accumulation of proteins are toxic elements in the progression of a broad range of neurodegenerative diseases. Molecular chaperones enable a cellular defense by reducing or compartmentalizing these insults. Small heat shock proteins (sHsps) engage proteins early in the process of misfolding and can facilitate their proper folding or refolding, sequestration, or clearance. Here, we evaluate the effects of the sHsp Hsp22, as well as a pseudophosphorylated mutant and an N-terminal domain deletion (NTDΔ) variant on tau aggregation in vitro and tau accumulation and aggregation in cultured cells. Hsp22 wild-type (WT) protein had a significant inhibitory effect on heparin-induced aggregation in vitro and the pseudophosphorylated mutant Hsp22 demonstrated a similar effect. When co-expressed in a cell culture model with tau, these Hsp22 constructs significantly reduced soluble tau protein levels when transfected at a high ratio relative to tau. However, the Hsp22 NTDΔ protein drastically reduced the soluble protein expression levels of both tau WT and tau P301L/S320F even at lower transfection ratios, which resulted in a correlative reduction of the triton-insoluble tau P301L/S320F aggregates.
Proteomic Analysis of Peri-Wounding Tissue Expressions in Extracorporeal Shock Wave Enhanced Diabetic Wound Healing in a Streptozotocin-Induced Diabetes Model
Authors: Chen Rong-Fu , Yang Ming-Yu , Wang Ching-Jen , Wang Chun-Ting , Kuo Yur-Ren
Published: July 2020

Our former studies have demonstrated that extracorporeal shock wave therapy (ESWT) could enhance diabetic wound healing but the bio-mechanisms remain elusive. This study investigated the changes of topical peri-wounding tissue expressions after ESWT in a rodent streptozotocin-induced diabetic wounding model by using the proteomic analysis and elucidated the molecular mechanism. Diabetic rats receiving ESWT, normal control, and diabetic rats receiving no therapy were analyzed. The spots of interest in proteome analysis were subjected to mass spectrometry to elucidate the peptide mass fingerprints. Protein expression was validated using immunohistochemical staining and related expression of genes were analyzed using real-time RT-PCR. The proteomic data showed a significantly higher abundance of hemopexin at day 3 of therapy but down-regulation at day 10 as compared to diabetic control. In contrast, the level of serine proteinase inhibitor (serpin) A3N expression was significantly decreased at day 3 therapy but expression was upregulated at day 10. Using real-time RT-PCR revealed that serpin-related EGFR-MAPK pathway was involved in ESWT enhanced diabetic wound healing. In summary, proteome analyses demonstrated the expression change of hemopexin and serpin with related MAPK signaling involved in ESWT-enhanced diabetic wound healing. Modulation of hemopexin and serpin related pathways are good strategies to promote wound healing.
Intra-Articular Injection of Stromal Cell-Derived Factor 1α Promotes Meniscal Healing via Macrophage and Mesenchymal Stem Cell Accumulation in a Rat Meniscal Defect Model
Authors: Nishida Yohei , Hashimoto Yusuke , Orita Kumi , Nishino Kazuya , Kinoshita Takuya , Nakamura Hiroaki
Published: July 2020

The stromal-cell-derived factor-1α (SDF-1) is well-known for playing important roles in the regeneration of tissue by enhancing cell migration. However, the effect of SDF-1 in meniscal healing remains unknown. The purpose of this study is to investigate the effects of intra-articular injection of SDF-1 on meniscus healing in a rat meniscal defect model. The intra-articular SDF-1 injection was performed at meniscectomy and one week later. Macroscopic and histological assessments of the reparative meniscus were conducted at one, two and six weeks after meniscectomy in rats. In the macroscopic evaluation, the SDF-1 group showed an increase in the size of the reparative meniscus at six weeks after meniscectomy compared to the phosphate-buffered saline (PBS) injection (no-treatment) group. Histological findings showed that intra-articular injection of SDF-1 enhanced the migration of macrophages to the site of the regenerative meniscus at one and two weeks after meniscectomy. CD68- and CD163-positive cells in the SDF-1 group at one week after meniscectomy were significantly higher than in the no-treatment group. CD163-positive cells in the SDF-1 group at two weeks were significantly higher than in the no-treatment group. At one week after meniscectomy, there were cells expressing mesenchymal-stem-cell-related markers in the SDF-1 group. These results indicate the potential of regenerative healing of the meniscus by SDF-1 injection via macrophage and mesenchymal stem cell accumulation. In the present study, intra-articular administration of SDF-1 contributed to meniscal healing via macrophage, CD90-positive cell and CD105-positive cell accumulation in a rat meniscal defect model. The SDF-1–CXCR4 pathway plays an important role in the meniscal healing process. For potential clinical translation, SDF-1 injection therapy seems to be a promising approach for the biological augmentation in meniscal injury areas to enhance healing capacity.
Effects of Eicosapentaenoic Acid on Arterial Calcification
Authors: Saito Yukihiro , Nakamura Kazufumi , Ito Hiroshi
Published: July 2020

Arterial calcification is a hallmark of advanced atherosclerosis and predicts cardiovascular events. However, there is no clinically accepted therapy that prevents progression of arterial calcification. HMG-CoA reductase inhibitors, statins, lower low-density lipoprotein-cholesterol and reduce cardiovascular events, but coronary artery calcification is actually promoted by statins. The addition of eicosapentaenoic acid (EPA) to statins further reduced cardiovascular events in clinical trials, JELIS and REDUCE-IT. Additionally, we found that EPA significantly suppressed arterial calcification in vitro and in vivo via suppression of inflammatory responses, oxidative stress and Wnt signaling. However, so far there is a lack of evidence showing the effect of EPA on arterial calcification in a clinical situation. We reviewed the molecular mechanisms of the inhibitory effect of EPA on arterial calcification and the results of some clinical trials.
A Curcumin Analog Exhibits Multiple Biologic Effects on the Pathogenesis of Alzheimer’s Disease and Improves Behavior, Inflammation, and β-Amyloid Accumulation in a Mouse Model
Authors: Su Ih-Jen , Chang Hong-Yi , Wang Hui-Chen , Tsai Kuen-Jer
Published: July 2020

Drugs for the treatment of Alzheimer’s disease (AD) are in urgent demand due to the unmet need and the social burden associated with the disease. Curcumin has been historically considered as a beneficial product for anti-aging and AD. However, many efforts to develop curcumin for clinical use are hindered mainly due to its poor bioavailability. Recent development in drug delivery and structural design has resolved these issues. In this study, we identified a small molecule, TML-6, as a potential drug candidate for AD through screening a panel of curcumin derivatives using six biomarker platforms related to aging biology and AD pathogenesis. The structural modification of TML-6 is designed to improve the stability and metabolism of curcumin. Cell biological studies demonstrated that TML-6 could inhibit the synthesis of the β-amyloid precursor protein and β-amyloid (Aβ), upregulate Apo E, suppress NF-κB and mTOR, and increase the activity of the anti-oxidative Nrf2 gene. In the 3x-Tg AD animal model, TML-6 treatment resulted in significant improvement in learning, suppression of the microglial activation marker Iba-1, and reduction in Aβ in the brain. Although TML-6 exhibited a greater improvement in bioavailability as compared to curcumin, formulation optimization and toxicological studies are under development to assure its druggability. Taken together, TML-6 meets the current strategy to develop therapeutics for AD, targeting the combination of the Aβ cascade and aging-related biology processes.
A Modified Protocol of Diethylnitrosamine Administration in Mice to Model Hepatocellular Carcinoma
Authors: Memon Azra , Pyao Yuliya , Jung Yerin , Lee Jung Il , Lee Woon Kyu
Published: July 2020

We aimed to create an animal model for hepatocellular carcinoma (HCC) with a short time, a high survival rate, as well as a high incidence of HCC in both males and females than previously reported. The Diethylnitrosamine (DEN) model has an age-related effect. A single dose of DEN treatment is not enough in young mice up to 50 weeks. The same pattern is shown in an adult with multiple-dose trials whether or not there is some promotion agent. In this study, two-week old C57BL6 mice were given a total of eight doses of DEN, initially 20mg/kg body weight, and then 30mg/kg in the third week, followed by 50mg/kg for the last six weeks. The first group is DEN treatment only and the other two groups received thioacetamide (TAA) treatment for four or eight weeks after one week of rest from the last DEN treatment. An autopsy was performed after 24 weeks of the initial dose of DEN in each group. The cellular arrangement of HCC in the entire group was well-differentiated carcinoma and tumor presence with no significant impact on the survival of mice. Increased levels of the biochemical markers in serum, loss of tissue architecture, hepatocyte death, and proliferation were highly activated in all tumor-induced groups. This finding demonstrates an improved strategy to generate an animal model with a high occurrence of tumors combined with cirrhosis in a short time regardless of sex for researchers who want to investigate liver cancer-related.
Insights into the Effect of Curcumin and (–)-Epigallocatechin-3-Gallate on the Aggregation of Aβ(1–40) Monomers by Means of Molecular Dynamics
Authors: Tavanti Francesco , Pedone Alfonso , Menziani Maria Cristina
Published: July 2020

In this study, we compared the effects of two well-known natural compounds on the early step of the fibrillation process of amyloid-β (1–40), responsible for the formation of plaques in the brains of patients affected by Alzheimer’s disease (AD). The use of extensive replica exchange simulations up to the µs scale allowed us to characterize the inhibition activity of (–)-epigallocatechin-3-gallate (EGCG) and curcumin (CUR) on unfolded amyloid fibrils. A reduced number of β-strands, characteristic of amyloid fibrils, and an increased distance between the amino acids that are responsible for the intra- and interprotein aggregations are observed. The central core region of the amyloid-β (Aβ(1–40)) fibril is found to have a high affinity to EGCG and CUR due to the presence of hydrophobic residues. Lastly, the free binding energy computed using the Poisson Boltzmann Surface Ares suggests that EGCG is more likely to bind to unfolded Aβ(1–40) fibrils and that this molecule can be a good candidate to develop new and more effective congeners to treat AD.
High-Throughput Sequencing and Expression Analysis Suggest the Involvement of Pseudomonas putida RA-Responsive microRNAs in Growth and Development of Arabidopsis
Authors: Jatan Ram , Chauhan Puneet Singh , Lata Charu
Published: July 2020

Beneficial soil microorganisms largely comprise of plant growth-promoting rhizobacteria (PGPR), which adhere to plant roots and facilitate their growth and development. Pseudomonas putida (RA) strain MTCC5279 is one such PGPR that exhibits several characteristics of plant growth promotion, such as P-solubilization, and siderophores and IAA production. Plant–PGPR interactions are very complex phenomena, and essentially modulate the expression of numerous genes, consequently leading to changes in the physiological, biochemical, cellular and molecular responses of plants. Therefore, in order to understand the molecular bases of plant–PGPR interactions, we carried out the identification of microRNAs from the roots of Arabidopsis upon P. putida RA-inoculation, and analyses of their expression. MicroRNAs (miRNAs) are 20- to 24-nt non-coding small RNAs known to regulate the expression of their target genes. Small RNA sequencing led to the identification of 293 known and 67 putative novel miRNAs, from the control and RA-inoculated libraries. Among these, 15 known miRNAs showed differential expression upon RA-inoculation in comparison to the control, and their expressions were corroborated by stem-loop quantitative real-time PCR. Overall, 28,746 and 6931 mRNAs were expected to be the targets of the known and putative novel miRNAs, respectively, which take part in numerous biological, cellular and molecular processes. An inverse correlation between the expression of RA-responsive miRNAs and their target genes also strengthened the crucial role of RA in developmental regulation. Our results offer insights into the understanding of the RA-mediated modulation of miRNAs and their targets in Arabidopsis, and pave the way for the further exploitation and characterization of candidate RA-responsive miRNA(s) for various crop improvement strategies directed towards plant sustainable growth and development.
Sexual Dimorphism in the Multielemental Stoichiometric Phenotypes and Stoichiometric Niches of Spiders
Authors: Sobczyk Łukasz , Filipiak Michał , Czarnoleski Marcin
Published: July 2020

Nutritional limitations may shape populations and communities of organisms. This phenomenon is often studied by treating populations and communities as pools of homogenous individuals with average nutritional optima and experiencing average constraints and trade-offs that influence their fitness in a standardized way. However, populations and communities consist of individuals belonging to different sexes, each with specific nutritional demands and limitations. Taking this into account, we used the ecological stoichiometry framework to study sexual differences in the stoichiometric phenotypes, reflecting stoichiometric niches, of four spider taxa differing in the hunting mode. The species and sexes differed fundamentally in their elemental phenotypes, including elements beyond those most commonly studied (C, N and P). Both species and sexes were distinguished by the C:N ratio and concentrations of Cu, K and Zn. Species additionally differed in concentrations of Na, Mg and Mn. Phosphorous was not involved in this differentiation. Sexual dimorphism in spiders’ elemental phenotypes, related to differences in their stoichiometric niches, suggests different nutritional optima and differences in nutritional limitation experienced by different sexes and species. This may influence the structure and functioning of spider populations and communities.
AI-Inspired Non-Terrestrial Networks for IIoT: Review on Enabling Technologies and Applications
Authors: Michailidis Emmanouel T. , Potirakis Stelios M. , Kanatas Athanasios G.
Published: July 2020

During the last few years, various Industrial Internet of Things (IIoT) applications have emerged with numerous network elements interconnected using wired and wireless communication technologies and equipped with strategically placed sensors and actuators. This paper justifies why non-terrestrial networks (NTNs) will bring the IIoT vision closer to reality by providing improved data acquisition and massive connectivity to sensor fields in large and remote areas. NTNs are engineered to utilize satellites, airships, and aircrafts, which can be employed to extend the radio coverage and provide remote monitoring and sensing services. Additionally, this paper describes indicative delay-tolerant massive IIoT and delay-sensitive mission-critical IIoT applications spanning a large number of vertical markets with diverse and stringent requirements. As the heterogeneous nature of NTNs and the complex and dynamic communications scenarios lead to uncertainty and a high degree of variability, conventional wireless communication technologies cannot sufficiently support ultra-reliable and low-latency communications (URLLC) and offer ubiquitous and uninterrupted interconnectivity. In this regard, this paper sheds light on the potential role of artificial intelligence (AI) techniques, including machine learning (ML) and deep learning (DL), in the provision of challenging NTN-based IIoT services and provides a thorough review of the relevant research works. By adding intelligence and facilitating the decision-making and prediction procedures, the NTNs can effectively adapt to their surrounding environment, thus enhancing the performance of various metrics with significantly lower complexity compared to typical optimization methods.
A Panel of Broad-Spectrum Antivirals in Topical Ophthalmic Medications from the Drug Repurposing Approach during and after the Coronavirus Disease 2019 Era
Authors: Napoli Pietro Emanuele , Mangoni Lorenzo , Gentile Pietro , Braghiroli Mirco , Fossarello Maurizio
Published: July 2020

The coronavirus disease 2019 (COVID-19) represents a global concern of public health caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Its clinical manifestations are characterized by a heterogeneous group of symptoms and pictures (ranging from asymptomatic to lethal courses). The prevalence of conjunctivitis in patients with COVID-19 is at present controversial. Although it has been reported that only 0.9% developed signs of conjunctivitis, other report indicates that up to 31.6% of hospitalized patients had conjunctivitis. Considering the widespread use of topical ophthalmic medications (e.g., eye drops) by the general population, for various reasons (e.g., artificial tears, anti-glaucoma medications, topical antibiotics, etc.), the existence of their side effects as antiviral action should be investigated in-depth because it could possibly explain the aforementioned controversial data and represent a potential antiviral treatment for SARS-CoV-2 replication/diffusion on the ocular surface. Here, we discuss and elucidate the antiviral side effect of many eye drops and ophthalmic ointments commonly used for others purposes, thus showing that these secondary effects (not to be confused with the ‘adverse effects’) might be of primary importance in a number of viral infections (e.g., those for which there is no validated treatment protocol), according to a drug repurposing approach. Some active ingredients or excipients described here have activity against other types of viruses, thus suggesting potential broad-spectrum applications.
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: Efficacy of Repeat Immunoadsorption
Authors: Tölle Markus , Freitag Helma , Antelmann Michaela , Hartwig Jelka , Schuchardt Mirjam , van der Giet Markus , Eckardt Kai-Uwe , Grabowski Patricia , Scheibenbogen Carmen
Published: July 2020

(1) Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex neuroimmunological disease. There is evidence for an autoimmune mechanism for ME/CFS with an infection-triggered onset and dysfunction of ß2-adrenoreceptor antibodies (ß2AR-AB). In a first proof-of-concept study, we could show that IA was effective to reduce ß2AR-AB and led to improvement of various symptoms. (2) Five of the ME/CFS patients who had clinical improvement following treatment with a five-day IA were retreated in the current study about two years later with a modified IA protocol. The severity of symptoms was assessed by disease specific scores during a follow-up period of 12 months. The antibodies were determined by ELISA. (3) The modified IA treatment protocol resulted in a remarkable similar clinical response. The treatment was well tolerated and 80–90% decline of total IgG and ß2AR-AB was achieved. Four patients showed a rapid improvement in several clinical symptoms during IA therapy, lasting for six to 12 months. One patient had no improvement. (4) We could provide further evidence that IA has clinical efficacy in patients with ME/CFS. Data from our pilot trial warrant further controlled studies in ME/CFS.
Risk for Depressive Symptoms among Hospitalized Women in High-Risk Pregnancy Units during the COVID-19 Pandemic
Authors: Sade Shanny , Sheiner Eyal , Wainstock Tamar , Hermon Narkis , Yaniv Salem Shimrit , Kosef Tamar , Lanxner Battat Talya , Oron Sharon , Pariente Gali
Published: July 2020

Objective: Higher rates of mental disorders, specifically depression, were found among affected people in previous epidemiological studies taken after disasters. The aim of the current study was to assess risk for depression among pregnant women hospitalized during the “coronavirus disease 2019” (COVID-19) pandemic, as compared to women hospitalized before the COVID-19 pandemic. Study design: A cross-sectional study was performed among women hospitalized in the high-risk pregnancy units of the Soroka University Medical Center (SUMC). All participating women completed the Edinburgh Postnatal Depression Scale (EPDS), and the results were compared between women hospitalized during the COVID-19 strict isolation period (19 March 2020 and 26 May 2020) and women hospitalized before the COVID-19 pandemic. Multivariable logistic regression models were constructed to control for potential confounders. Results: Women hospitalized during the COVID-19 strict isolation period (n = 84) had a comparable risk of having a high (>10) EPDS score as compared to women hospitalized before the COVID-19 pandemic (n = 279; 25.0% vs. 29.0%, p = 0.498). These results remained similar in the multivariable logistic regression model, while controlling for maternal age, ethnicity and known mood disorder (adjusted odds ratio (OR) 1.0, 95% CI 0.52–1.93, p = 0.985). Conclusion: Women hospitalized at the high-risk pregnancy unit during the COVID-19 strict isolation period were not at increased risk for depression, as compared to women hospitalized before the COVID-19 pandemic.
The Animal Kingdom, Agriculture⋯ and Seaweeds
Authors: Cornish Melania L. , Monagail Michéal Mac , Critchley Alan T.
Published: July 2020

Marine macroalgae (seaweeds), are amongst the first multicellular organisms and, as such, the precursors to land plants. By the time ‘land’ animals arrived on the scene, terrestrial plants were plentiful and varied, and herbivorous diets developed in concert with the food sources most commonly available. However, skip forward several hundred millennia, and with the advent of agriculture, approximately 10,000 years ago, dietary diversity began to change. Today, the world is experiencing increasingly higher rates of debilitating, non-communicable diseases—might there be a connection? This paper reviews scientific evidence for the judicious use of various seaweeds in the reduction of heat stress, enhanced immunity, improved growth performance, and methane reduction in animals. The extensive, (super) prebiotic effects of selected macroalgae will also be highlighted. Key studies conducted across the animal kingdom provide considerable support that there is an overwhelming need for the guided and wise applications of increased usage of selected seaweeds in feed, food and supplements. Particular attention will be paid to the bioactive components, and nutraceutical qualities, of various seaweeds, i.e., the brown, Saccharina (Laminaria) spp. and Ascophyllum nodosum, and the red alga Chondrus crispus. Suggestions are put forward for benefits to be derived from their further applications.
Combined Floating Offshore Wind and Solar PV
Authors: López Mario , Rodríguez Noel , Iglesias Gregorio
Published: July 2020

To mitigate the effects of wind variability on power output, hybrid systems that combine offshore wind with other renewables are a promising option. In this work we explore the potential of combining offshore wind and solar power through a case study in Asturias (Spain)—a region where floating solutions are the only option for marine renewables due to the lack of shallow water areas, which renders bottom-fixed wind turbines inviable. Offshore wind and solar power resources and production are assessed based on high-resolution data and the technical specifications of commercial wind turbines and solar photovoltaic (PV) panels. Relative to a typical offshore wind farm, a combined offshore wind–solar farm is found to increase the capacity and the energy production per unit surface area by factors of ten and seven, respectively. In this manner, the utilization of the marine space is optimized. Moreover, the power output is significantly smoother. To quantify this benefit, a novel Power Smoothing (PS) index is introduced in this work. The PS index achieved by combining floating offshore wind and solar PV is found to be of up to 63%. Beyond the interest of hybrid systems in the case study, the advantages of combining floating wind and solar PV are extensible to other regions where marine renewable energies are being considered.
Modulation of Th1/Th2 Cytokine Balance by Quercetin In Vitro
Authors: Tanaka Yoshihito , Furuta Atsuko , Asano Kazuhito , Kobayashi Hitome
Published: July 2020

Background: Allergic rhinitis (AR) is well known to be an IgE-mediated chronic inflammatory disease in the nasal wall, which is primarily mediated by Th2-type cytokines such as IL-4, IL-5, and IL-13. Although quercetin is also accepted to attenuate the development of allergic diseases such as AR, the influence of quercetin on Th2-type cytokine production is not well understood. The present study was designed to examine whether quercetin could attenuate the development of AR via the modulation of Th2-type cytokine production using an in vitro cell culture technique. Methods: Human peripheral-blood CD4+ T cells (1 × 106 cells/mL) were cultured with 10.0 ng/mL IL-4 in the presence or absence of quercetin. The levels of IL-5, IL-13, and INF-γ in 24 h culture supernatants were examined by ELISA. The influence of quercetin on the phosphorylation of transcription factors NF-κB and STAT6, and mRNA expression for cytokines were also examined by ELISA and RT-PCR, respectively. Results: Treatment of cells with quercetin at more than 5.0 μM inhibited the production of IL-5 and IL-13 from CD4+ T cells induced by IL-4 stimulation through the suppression of transcription factor activation and cytokine mRNA expression. On the other hand, quercetin at more than 5.0 μM abrogated the inhibitory action of IL-4 on INF-γ production from CD4+ T cells in vitro. Conclusions: The immunomodulatory effects of quercetin, especially on cytokine production, may be responsible, in part, for the mode of therapeutic action of quercetin on allergic diseases, including AR.
The Impact of Process Factors on Creating Defects, Mainly Lustrous Carbon, during the Production of Ductile Iron Using the Lost-Foam Casting (LFC) Method
Authors: Jezierski Jan , Jureczko Michał , Dojka Rafał
Published: July 2020

The purpose of this paper was to analyze the process factors affecting the occurrence of lustrous carbon defects in ductile cast iron castings when using the lost-foam casting (LFC) method. This phenomenon results in creating raw surface defects, which sometimes may even lead to cast iron scrapping. A series of trial melting batches were carried out for variable process assumptions. The analysis was performed to reflect, to the greatest extent possible, real foundry production conditions. Industrial tests were performed in Odlewnia Rafamet Sp. z o.o., Kuźnia Raciborska, Poland. The polystyrene patterns created by gluing components together, used in the tests, met the requirements of the high-tech lost-foam casting (LFC) process. The performed analysis allowed the obtaining of lustrous carbon defects in test castings as well as the determination of the process parameters with the highest impact on lustrous carbon inclusions in ductile iron castings. The test results were used to eliminate the possibility of creating a defect and thus directly improve the efficiency of the lost-foam casting (LFC) process used in the foundry.
Effects of Anisotropy on Single Crystal Silicon in Polishing Non-Continuous Surface
Authors: Wang Guilian , Feng Zhijian , Hu Yahui , Liu Jie , Zheng Qingchun
Published: July 2020

A molecular dynamics model of the diamond abrasive polishing the single crystal silicon is established. Crystal surfaces of the single crystal silicon in the Y-direction are (010), (011), and (111) surfaces, respectively. The effects of crystallographic orientations on polishing the non-continuous single crystal silicon surfaces are discussed from the aspects of surface morphology, displacement, polishing force, and phase transformation. The simulation results show that the Si(010) surface accumulates chips more easily than Si(011) and Si(111) surfaces. Si(010) and Si(011) workpieces are deformed in the entire pore walls on the entry areas of pores, while the Si(111) workpiece is a local large deformation on entry areas of the pores. Comparing the recovery value of the displacement in different workpieces, it can be seen that the elastic deformation of the A side in the Si(011) workpiece is larger than that of the A side in other workpieces. Pores cause the tangential force and normal force to fluctuate. The fluctuation range of the tangential force is small, and the fluctuation range of the normal force is large. Crystallographic orientations mainly affect the position where the tangential force reaches the maximum and minimum values and the magnitude of the decrease in the tangential force near the pores. The position of the normal force reaching the maximum and minimum values near the pores is basically the same, and different crystallographic orientations have no obvious effect on the drop of the normal force, except for a slight fluctuation in the value. The high-pressure phase transformation is the main way to change the crystal structure. The Si(111) surface is the cleavage surface of single crystal silicon, and the total number of main phase transformation atoms on the Si(111) surface is the largest among the three types of workpieces. In addition, the phase transformation in Si(010) and Si(011) workpieces extends to the bottom of pores, and the Si(111) workpiece does not extend to the bottom of pores.
Droplet Generation in a Flow-Focusing Microfluidic Device with External Mechanical Vibration
Authors: Yin Zhaoqin , Huang Zemin , Lin Xiaohui , Gao Xiaoyan , Bao Fubing
Published: July 2020

The demand for highly controllable droplet generation methods is very urgent in the medical, materials, and food industries. The droplet generation in a flow-focusing microfluidic device with external mechanical vibration, as a controllable droplet generation method, is experimentally studied. The effects of vibration frequency and acceleration amplitude on the droplet generation are characterized. The linear correlation between the droplet generation frequency and the external vibration frequency and the critical vibration amplitude corresponding to the imposing vibration frequency are observed. The droplet generation frequency with external mechanical vibration is affected by the natural generation frequency, vibration frequency, and vibration amplitude. The droplet generation frequency in a certain microfluidic device with external vibration is able to vary from the natural generation frequency to the imposed vibration frequency at different vibration conditions. The evolution of dispersed phase thread with vibration is remarkably different with the process without vibration. Distinct stages of expansion, shrinkage, and collapse are observed in the droplet formation with vibration, and the occurrence number of expansion–shrinkage process is relevant with the linear correlation coefficient.
Curved Structure of Si by Improving Etching Direction Controllability in Magnetically Guided Metal-Assisted Chemical Etching
Authors: Kim Tae , Bae Jee-Hwan , Kim Juyoung , Cho Min , Kim Yu-Chan , Jin Sungho , Chun Dongwon
Published: July 2020

Metal-assisted chemical etching (MACE) is widely used to fabricate micro-/nano-structured Si owing to its simplicity and cost-effectiveness. The technique of magnetically guided MACE, involving MACE with a tri-layer metal catalyst, was developed to improve etching speed as well as to adjust the etching direction using an external magnetic field. However, the controllability of the etching direction diminishes with an increase in the etching dimension, owing to the corrosion of Fe due to the etching solution; this impedes the wider application of this approach for the fabrication of complex micro Si structures. In this study, we modified a tri-layer metal catalyst (Au/Fe/Au), wherein the Fe layer was encapsulated to improve direction controllability; this improved controllability was achieved by protecting Fe against the corrosion caused by the etching solution. We demonstrated curved Si microgroove arrays via magnetically guided MACE with Fe encapsulated in the tri-layer catalyst. Furthermore, the curvature in the curved Si microarrays could be modulated via an external magnetic field, indicating that direction controllability could be maintained even for the magnetically guided MACE of bulk Si. The proposed fabrication method developed for producing curved Si microgroove arrays can be applied to electronic devices and micro-electromechanical systems.