bluuebunny/crossref_metadata_2025 huggingface dataset:
Expertise Classification of Soccer Goalkeepers in Highly Dynamic Decision Tasks: A Deep Learning Approach for Temporal and Spatial Feature Recognition of Fixation Image Patch Sequences
Authors: Hosp Benedikt , Schultz Florian , Kasneci Enkelejda , Höner Oliver
Published: July 2021

The focus of expertise research moves constantly forward and includes cognitive factors, such as visual information perception and processing. In highly dynamic tasks, such as decision making in sports, these factors become more important to build a foundation for diagnostic systems and adaptive learning environments. Although most recent research focuses on behavioral features, the underlying cognitive mechanisms have been poorly understood, mainly due to a lack of adequate methods for the analysis of complex eye tracking data that goes beyond aggregated fixations and saccades. There are no consistent statements about specific perceptual features that explain expertise. However, these mechanisms are an important part of expertise, especially in decision making in sports games, as highly trained perceptual cognitive abilities can provide athletes with some advantage. We developed a deep learning approach that independently finds latent perceptual features in fixation image patches. It then derives expertise based solely on these fixation patches, which encompass the gaze behavior of athletes in an elaborately implemented virtual reality setup. We present a CNN-BiLSTM based model for expertise assessment in goalkeeper-specific decision tasks on initiating passes in build-up situations. The empirical validation demonstrated that our model has the ability to find valuable latent features that detect the expertise level of 33 athletes (novice, advanced, and expert) with 73.11% accuracy. This model is a first step in the direction of generalizable expertise recognition based on eye movements.
Ontogenetic Change in Behavioral Responses to Structural Enrichment From Fry to Parr in Juvenile Atlantic Salmon (Salmo salar L.)
Authors: Alnes Ingeborg Bjerkvik , Jensen Knut Helge , Skorping Arne , Salvanes Anne Gro Vea
Published: July 2021

Enrichment is widely used as a tool for studying how changes in environment affect animal behavior. Here, we report an experimental study investigating if behaviors shaped by stimuli from environmental enrichment depending on the stage animals are exposed to enrichment. We used juvenile Atlantic salmon (Salmo salar) in their first autumn. This is a species commonly reared for conservation purposes. Previous work has shown that environmental enrichment had no effect on long-term survival when the fry stage (smaller than 70 mm) was released, but that if late parr stages (larger than 70 mm) are released, enrichment is reported to have a positive effect on smolt migration survival. Here, we explored the effect of enrichment at two different stages of development. Both stages were reared and treated for 7 weeks (fry at 11–18 weeks and parr at 24–31 weeks after hatching) before tested for behavior. Responses known to be associated with exploratory behavior, activity, and stress coping were quantified by testing 18-week-old fry and 31-week-old parr in a six-chamber maze on 7 successive days after rearing in structurally enriched (plastic plants and tubes) or plain impoverished rearing environments. The data show that Atlantic salmon are sensitive to stimuli from structural enrichment when they are parr, but not when in the fry stage. Parr deprived of enrichment (control treatment) were reluctant to start exploring the maze, and when they did, they spent a longer time frozen than enriched parr, suggesting that deprivation of enrichment at this life can be stressful. Our data suggest that structural enrichment could have the potential to improve welfare for salmonids in captivity and for survival of released juvenile salmon if structural enrichment is provided at the parr stage and the fish reared for conservation are released at the parr stage.
Amino Acid Supplementation to Reduce Environmental Impacts of Broiler and Pig Production: A Review
Authors: Cappelaere Léa , Le Cour Grandmaison Josselin , Martin Nicolas , Lambert William
Published: July 2021

Poultry and swine farming are large contributors to environmental impacts, such as climate change, eutrophication, acidification, and air and water pollution. Feed production and manure management are identified as the main sources of these impacts. Reducing dietary crude protein levels is a nutritional strategy recognized to both decrease the use of high-impact feed ingredients and alter manure composition, reducing emissions of harmful components. For a successful implementation of this technique, feed-grade amino acid supplementation is crucial to maintaining animal performance. Reducing crude protein lowers nitrogen excretion, especially excess nitrogen excreted in urea or uric acid form, improving nitrogen efficiency. At the feed-gate, low–crude protein diets can reduce the carbon footprint of feed production through changes in raw material inclusion. The magnitude of this reduction mainly depends on the climate change impact of soybean meal and its land-use change on the feed-grade amino acids used. Reducing dietary crude protein also lowers the environmental impact of manure management in housing, storage, and at spreading: nitrogen emissions from manure (ammonia, nitrates, nitrous oxide) are reduced through reduction of nitrogen excretion. Moreover, synergetic effects exist with nitrogen form, water excretion, and manure pH, further reducing emissions. Volatilization of nitrogen is more reduced in poultry than in pigs, but emissions are more studied and better understood for pig slurry than poultry litter. Ammonia emissions are also more documented than other N-compounds. Low–crude protein diets supplemented with amino acids is a strategy reducing environmental impact at different stages of animal production, making life cycle assessment the best-suited tool to quantify reduction of environmental impacts. Recent studies report an efficient reduction of environmental impacts with low–crude protein diets. However, more standardization of limits and methods used is necessary to compare results. This review summarizes the current knowledge on mitigation of environmental impacts with low–crude protein diets supplemented with amino acids in poultry and swine, its quantification, and the biological mechanisms involved. A comparison between pigs and poultry is also included. It provides concrete information based on quantified research for decision making for the livestock industry and policy makers.
Epidemiology of Pig Tuberculosis in Argentina
Authors: Barandiaran Soledad , Marfil María Jimena , Capobianco Guillermo , Pérez Aguirreburualde María Sol , Zumárraga Martín José , Eirin María Emilia , Cuerda María Ximena , Winter Marina , Martínez Vivot Marcela , Perez Andres Maximiliano , La Sala Luciano Francisco
Published: July 2021

Bovine tuberculosis (bTB) is a disease caused mainly by theMycobacterium bovisand that is endemic to livestock populations in most Latin American countries. Traditionally, bTB control programs are costly and targeted to cattle, largely disregarding other species such as swine and wildlife. According to official services, in Argentina disease prevalence in pigs is comparable to that observed in cattle, suggesting the need for efficient control programs to manage the disease in both species. Additionally, extensive farming systems, which are commonly practiced in Argentina, allow the interaction between livestock and wildlife such as wild boar (Sus scrofa), which is considered a natural host of the disease. Here, we evaluated the bTB pigs- cattle interface, studying the dynamics ofM. bovisisolates in the pig population and identifying farm-level epidemiological variables associated with the disease confirmation at slaughterhouses. Additionally, to assess the potential multi-host systems in the transmission of bTB, the molecular characterization of wild boar mycobacterial strains was included in the study, as this interaction has not been previously evaluated in this region. Multivariable logistic regression models were used to assess the association between farm-level epidemiological variables (location, farm size, and co-existence with cattle and goats) and bTB confirmation in pig tuberculosis-like lesions samples. Results showed that when cattle were present, the odds of bTB in pigs decreased 0.3 or 0.6% for every additional sow when cattle were present or absent in the farm, respectively. Pigs shared 60% (18/30) of the genotypes with cattle and wild boar, suggesting transmission at the interface between pigs and cattle and highlighting the potential role of wild boar in bTB maintenance. These results provide novel information about the molecular diversity ofM. bovisstrains in pigs in Argentina and proposes the potential relevance of a multi-host system in the epidemiology of bTB in the region. The statistical models presented here may be used in the design of a low cost, abattoir-based surveillance program for bTB in the pig industry in Argentina, with potential extension to other settings with similar epidemiological conditions.
Nitrogen Fractions in Soil Fertilized with Waste Organic Materials
Authors: Wierzbowska Jadwiga , Sienkiewicz Stanisław , Załuski Dariusz
Published: July 2021

The aim of the study was to determine the effect of sewage sludge and composts produced from sewage sludge and municipal waste on the content of various forms of nitrogen in soil. The field experiment was carried out in 2004–2015. It included three crop rotations of the following plants: potato, spring barley, winter oilseed rape and winter wheat. The experiment consisted of the following treatments: control (without fertilization), NPK, manure (FYM), compost from municipal sewage sludge and straw (CSSS), composted sewage sludge (CSS), dried and granulated sewage sludge (DGSS), “Dano” compost produced from unsorted municipal waste (CUMW) and compost from municipal green waste (CMGW). Manure, composts and sewage sludge were applied once (10 t ha−1 of d.m.) or twice (5 t ha−1 of d.m.) in a crop rotation. It was significantly shown that the highest N-total content was in the soil fertilized with CUMW (compost produced from unsorted municipal waste). The soil fertilized with manure (FYM) contained the highest quantity of N-min. The prevalent pool of nitrogen (82.65–86.52%) consisted of N compounds not undergoing acid hydrolysis, and their smallest share was determined in the soil fertilized with NPK alone.
Isoquinoline Alkaloids in Sows’ Diet Reduce Body Weight Loss during Lactation and Increase IgG in Colostrum
Authors: Arévalo Sureda Ester , Zhao Xuemei , Artuso-Ponte Valeria , Wall Sophie-Charlotte , Li Bing , Fang Wei , Uerlings Julie , Zhang Yuping , Schroyen Martine , Grelet Clément , Dehareng Frédéric , Wavreille José , Everaert Nadia
Published: July 2021

Isoquinoline alkaloids (IQ) exert beneficial antimicrobial and anti-inflammatory effects in livestock. Therefore, we hypothesized that supplementing sows’ diets with IQ during gestation would decrease farrowing stress, affecting the piglets’ development and performance. Sows were divided into: IQ1, supplemented with IQ from gestation day 80 (G80) to weaning; IQ2, supplemented from gestation day 110 (G110) to weaning, and a non-supplemented (NC) group. Sow body weight (BW), feed intake, back-fat thickness and back-muscle thickness were monitored. Cortisol, glucose and insulin were measured in sows’ blood collected 5 d before, during, and after 7 d farrowing. Protein, fat, IgA and IgG were analyzed in the colostrum and milk. Piglets were monitored for weight and diarrhea score, and for ileum histology and gene expression 5 d post-weaning. IQ-supplemented sows lost less BW during lactation. Glucose and insulin levels were lower in the IQ groups compared to NC-sows 5 d before farrowing and had higher levels of protein and IgG in their colostrum. No other differences were observed in sows, nor in the measured parameters in piglets. In conclusion, IQ supplementation affected sows’ metabolism, reducing body weight loss during lactation. Providing IQ to sows from their entrance into the maternity barn might be sufficient to induce these effects. IQ improved colostrum quality, increasing the protein and IgG content, improving passive immunity for piglets.
Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus)
Authors: Balboni Andrea , Urbani Lorenza , Delogu Mauro , Musto Carmela , Fontana Maria Cristina , Merialdi Giuseppe , Lucifora Giuseppe , Terrusi Alessia , Dondi Francesco , Battilani Mara
Published: July 2021

In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species.
Prevalence and Photobiology of Photosynthetic Dinoflagellate Endosymbionts in the Nudibranch Berghia stephanieae
Authors: Silva Ruben X. G. , Cartaxana Paulo , Calado Ricardo
Published: July 2021

Berghia stephanieae is a stenophagous sea slug that preys upon glass anemones, such as Exaiptasia diaphana. Glass anemones host photosynthetic dinoflagellate endosymbionts that sea slugs ingest when consuming E. diaphana. However, the prevalence of these photosynthetic dinoflagellate endosymbionts in sea slugs appears to be short-lived, particularly if B.stephanieae is deprived of prey that host these microalgae (e.g., during bleaching events impacting glass anemones). In the present study, we investigated this scenario, along with food deprivation, and validated the use of a non-invasive and non-destructive approach employing chlorophyll fluorescence as a proxy to monitor the persistence of the association between sea slugs and endosymbiotic photosynthetic dinoflagellates acquired through the consumption of glass anemones. Berghia stephanieae deprived of a trophic source hosting photosynthetic dinoflagellate endosymbionts (e.g., through food deprivation or by feeding on bleached E. diaphana) showed a rapid decrease in minimum fluorescence (Fo) and photosynthetic efficiency (Fv/Fm) when compared to sea slugs fed with symbiotic anemones. A complete loss of endosymbionts was observed within 8 days, confirming that no true symbiotic association was established. The present work opens a new window of opportunity to rapidly monitor in vivo and over time the prevalence of associations between sea slugs and photosynthetic dinoflagellate endosymbionts, particularly during bleaching events that prevent sea slugs from incorporating new microalgae through trophic interactions.
Epidemiology, Genetic Characterization, and Pathogenesis of Avian Influenza H5N8 Viruses Circulating in Northern and Southern Parts of Egypt, 2017–2019
Authors: Tarek Mohamed , Naguib Mahmoud M. , Arafa Abdel-Sattar , Tantawy Laila A. , Selim Karim M. , Talaat Shaimaa , Sultan Hesham A.
Published: July 2021

Highly pathogenic avian influenza (HPAI) viruses of subtype H5N8 continue to circulate, causing huge economic losses and serious impact on poultry production worldwide. Recently, HPAIV H5N8 has been spreading rapidly, and a large number of HPAI H5N8 outbreaks have been reported in Eurasia 2020–2021. In this study, we conducted an epidemiological survey of HPAI H5N8 virus at different geographical locations in Egypt from 2017 to 2019. This was followed by genetic and pathogenic studies. Our findings highlight the wide spread of HPAI H5N8 viruses in Egypt, including in 22 governorates. The genetic analyses of the hemagglutinin (HA) and neuraminidase (NA) gene segments emphasized a phylogenetic relatedness between the Egyptian HPAI H5N8 viruses and viruses of clade 2.3.4.4b recently isolated in Europe. These findings suggest that a potential back transmission of Egyptian HPAI H5N8 virus has occurred from domestic poultry in Egypt to migratory wild birds, followed by further spread to different countries. This highlights the importance of continuous epidemiological and genetic studies of AIVs at the domestic–wild bird interface.
Effects of Loblolly Pine Biochar and Wood Vinegar on Poultry Litter Nutrients and Microbial Abundance
Authors: Mohammadi-Aragh Maryam K. , Stokes C. Elizabeth , Street Jason T. , Linhoss John E.
Published: July 2021

Biochar, wood vinegar, and poultry litter are waste streams that can be utilized as soil amendments and fertilizers. However, poultry litter releases several pollutants through nutrient leaching and carries heavy microbial loads, including potential human pathogens. Improving nutrient retention and reducing microbial load in poultry litter may help protect environmental and human health and improve its value as a soil amendment. The objectives of this study were to determine how blending varying proportions of loblolly pine (Pinus taeda L.) biochar, wood vinegar, and poultry litter affected nutrient profiles and microbial abundance over time. Biochar inclusion rates were 0%, 5%, 10%, and 20%, and wood vinegar was applied at 2% w/w. Samples were taken at Day 0, 57, and 112 to measure nitrogen, phosphorus, potassium, pH, total fungi, and total bacteria. Nutrient levels generally decreased with increasing biochar level; however, biochar inclusion rates of 10% and 20% retained nitrogen and phosphorus and exhibited improved physical properties. Overall, adding wood vinegar decreased nutrient concentrations and showed a biocidal effect for bacteria and fungi. Bacteria and fungi showed different relationships with biochar inclusion rates, with fungi preferring higher biochar inclusion rates and bacteria flourishing at lower biochar inclusion rates.
Blockchain, Enterprise Resource Planning (ERP) and Accounting Information Systems (AIS): Research on e-Procurement and System Integration
Authors: Faccia Alessio , Petratos Pythagoras
Published: July 2021

Accounting information systems (AISs), the core module of any enterprise resource planning (ERP) system, are usually designed as centralised systems. Nowadays, the continuous development and applications of blockchain, or more broadly—distributed ledger technology (DLT), can change the architecture, overcome and improve some limitations of centralised systems, most notably security and privacy. An increasing number of authors are suggesting the application of blockchain technologies in management, accounting and ERPs. This paper aims to examine the emerging literature on this field, and an immediate result is that blockchain applications can have significant benefits. The paper’s innovative contribution and considerable objective are to examine if blockchain can be successfully integrated with AIS and ERPs. We find that blockchain can facilitate integration at multiple levels and better serve various purposes as auditing compliance. To demonstrate that, we analyse e-procurement systems and operations using case study research methodology. The findings suggest that DLT, decentralised finance (DeFI), and financial technology (FinTech) applications can facilitate integrating AISs and ERP systems and yield significant benefits for efficiency, productivity and security.
Study on Overturn Proof Monitoring System of Mobile Crane
Authors: Yoon Bok-Joong , Lee Kil-Soo , Lee Jae-Hak
Published: July 2021

While up-right build structures are under construction, an over-hung crane has a major role in efficient lifting and transporting heavy materials from one point to another. There are several types of cranes for a variety of construction sites, such as bridge/overhead, barge lift, tower crane, etc. The mobile crane is one of the most widely used types of construction equipment due to its mobility. Unfortunately, the number of crane accidents including casualties and deaths has increased over the last decade. In order to reduce these fatal tragedies, a dynamic simulator of mobile cranes based on analyzed overturn limit data has been developed and analysis results have been applied to site tests. The test bench is formulated to simulate the actual construction field and some practical experiments have been performed in realistic manners of operation. Moreover, wireless network communication systems are applied for monitoring the status of the crane from a distance where visibility is not secure. Consequently, the applicability in the field derived from operating the simulator and actual vehicle testing confirmed the feasibility of applying it to construction sites.
Regional and Local Impacts of the ENSO and IOD Events of 2015 and 2016 on the Indian Summer Monsoon—A Bhutan Case Study
Authors: Power Katherine , Axelsson Josefine , Wangdi Norbu , Zhang Qiong
Published: July 2021

The Indian Summer Monsoon (ISM) plays a vital role in the livelihoods and economy of those living on the Indian subcontinent, including the small, mountainous country of Bhutan. The ISM fluctuates over varying temporal scales and its variability is related to many internal and external factors including the El Niño Southern Oscillation (ENSO) and the Indian Ocean Dipole (IOD). In 2015, a Super El Niño occurred in the tropical Pacific alongside a positive IOD in the Indian Ocean and was followed in 2016 by a simultaneous La Niña and negative IOD. These events had worldwide repercussions. However, it is unclear how the ISM was affected during this time, both at a regional scale over the whole ISM area and at a local scale over Bhutan. First, an evaluation of data products comparing ERA5 reanalysis, TRMM and GPM satellite, and GPCC precipitation products against weather station measurements from Bhutan, indicated that ERA5 reanalysis was suitable to investigate ISM change in these two years. The reanalysis datasets showed that there was disruption to the ISM during this period, with a late onset of the monsoon in 2015, a shifted monsoon flow in July 2015 and in August 2016, and a late withdrawal in 2016. However, this resulted in neither a monsoon surplus nor a deficit across both years but instead large spatial-temporal variability. It is possible to attribute some of the regional scale changes to the ENSO and IOD events, but the expected impact of a simultaneous ENSO and IOD events are not recognizable. It is likely that 2015/16 monsoon disruption was driven by a combination of factors alongside ENSO and the IOD, including varying boundary conditions, the Pacific Decadal Oscillation, the Atlantic Multi-decadal Oscillation, and more. At a local scale, the intricate topography and orographic processes ongoing within Bhutan further amplified or dampened the already altered ISM.
Turbulent Momentum Flux Behavior above a Fire Front in an Open-Canopied Forest
Authors: Heilman Warren E. , Clark Kenneth L. , Bian Xindi , Charney Joseph J. , Zhong Shiyuan , Skowronski Nicholas S. , Gallagher Michael R. , Patterson Matthew
Published: July 2021

Atmospheric turbulent circulations in the vicinity of wildland fire fronts play an important role in the transfer of momentum into and out of combustion zones, which in turn can potentially affect the behavior and spread of wildland fires. The vertical turbulent transfer of momentum is accomplished via individual sweep, ejection, outward interaction, and inward interaction events, collectively known as sweep-ejection dynamics. This study examined the sweep-ejection dynamics that occurred before, during, and after the passage of a surface fire front during a prescribed fire experiment conducted in an open-canopied forest in the New Jersey Pine Barrens. High-frequency (10 Hz), tower-based, sonic anemometer measurements of horizontal and vertical wind velocity components in the vicinity of the fire front were used to assess the relative frequencies of occurrence of the different types of momentum-flux events, their contributions to the overall momentum fluxes, and their periodicity patterns. The observational results suggest that the presence of surface fire fronts in open-canopied forests can substantially change the sweep-ejection dynamics that typically occur when fires are not present. In particular, sweep events resulting in the downward transport of high horizontal momentum air from above were found to be more prominent during fire-front-passage periods.
Impact of Radar Reflectivity and Lightning Data Assimilation on the Rainfall Forecast and Predictability of a Summer Convective Thunderstorm in Southern Italy
Authors: Federico Stefano , Torcasio Rosa Claudia , Puca Silvia , Vulpiani Gianfranco , Comellas Prat Albert , Dietrich Stefano , Avolio Elenio
Published: July 2021

Heavy and localized summer events are very hard to predict and, at the same time, potentially dangerous for people and properties. This paper focuses on an event occurred on 15 July 2020 in Palermo, the largest city of Sicily, causing about 120 mm of rainfall in 3 h. The aim is to investigate the event predictability and a potential way to improve the precipitation forecast. To reach this aim, lightning (LDA) and radar reflectivity data assimilation (RDA) was applied. LDA was able to trigger deep convection over Palermo, with high precision, whereas the RDA had a key role in the prediction of the amount of rainfall. The simultaneous assimilation of both data sources gave the best results. An alert for a moderate–intense forecast could have been issued one hour and a half before the storm developed over the city, even if predicting only half of the total rainfall. A satisfactory prediction of the amount of rainfall could have been issued at 14:30 UTC, when precipitation was already affecting the city. Although the study is centered on a single event, it highlights the need for rapidly updated forecast cycles with data assimilation at the local scale, for a better prediction of similar events.
Surface Plasmon Resonance (SPR)- and Localized SPR (LSPR)-Based Virus Sensing Systems: Optical Vibration of Nano- and Micro-Metallic Materials for the Development of Next-Generation Virus Detection Technology
Authors: Takemura Kenshin
Published: July 2021

The global damage that a widespread viral infection can cause is evident from the ongoing COVID-19 pandemic. The importance of virus detection to prevent the spread of viruses has been reaffirmed by the pandemic and the associated social and economic damage. Surface plasmon resonance (SPR) in microscale and localized SPR (LSPR) in nanoscale virus sensing systems are thought to be useful as next-generation detection methods. Many studies have been conducted on ultra-sensitive technologies, especially those based on signal amplification. In some cases, it has been reported that even a low viral load can be measured, indicating that the virus can be detected in patients even in the early stages of the viral infection. These findings corroborate that SPR and LSPR are effective in minimizing false-positives and false-negatives that are prevalent in the existing virus detection techniques. In this review, the methods and signal responses of SPR and LSPR-based virus detection technologies are summarized. Furthermore, this review surveys some of the recent developments reported and discusses the limitations of SPR and LSPR-based virus detection as the next-generation detection technologies.
The Difficult Integration between Human and Animal Studies on Emotional Lateralization: A Perspective Article
Authors: Gainotti Guido
Published: July 2021

Even if for many years hemispheric asymmetries have been considered as a uniquely human feature, an increasing number of studies have described hemispheric asymmetries for various behavioral functions in several nonhuman species. An aspect of animal lateralization that has attracted particular attention has concerned the hemispheric asymmetries for emotions, but human and animal studies on this subject have been developed as independent lines of investigation, without attempts for their integration. In this perspective article, after an illustration of factors that have hampered the integration between human and animal studies on emotional lateralization, I will pass to analyze components and stages of the processing of emotions to distinguish those which point to a continuum between humans and many animal species, from those which suggest a similarity only between humans and great apes. The right lateralization of sympathetic functions (involved in brain and bodily activities necessary in emergency situations) seems consistent across many animal species, whereas asymmetries in emotional communication and in structures involved in emotional experience, similar to those observed in humans, have been documented only in primates.
Unsupervised Machine Learning to Identify Separable Clinical Alzheimer’s Disease Sub-Populations
Authors: Prakash Jayant , Wang Velda , Quinn Robert E. , Mitchell Cassie S.
Published: July 2021

Heterogeneity among Alzheimer’s disease (AD) patients confounds clinical trial patient selection and therapeutic efficacy evaluation. This work defines separable AD clinical sub-populations using unsupervised machine learning. Clustering (t-SNE followed by k-means) of patient features and association rule mining (ARM) was performed on the ADNIMERGE dataset from the Alzheimer’s Disease Neuroimaging Initiative (ADNI). Patient sociodemographics, brain imaging, biomarkers, cognitive tests, and medication usage were included for analysis. Four AD clinical sub-populations were identified using between-cluster mean fold changes [cognitive performance, brain volume]: cluster-1 represented least severe disease [+17.3, +13.3]; cluster-0 [−4.6, +3.8] and cluster-3 [+10.8, −4.9] represented mid-severity sub-populations; cluster-2 represented most severe disease [−18.4, −8.4]. ARM assessed frequently occurring pharmacologic substances within the 4 sub-populations. No drug class was associated with the least severe AD (cluster-1), likely due to lesser antecedent disease. Anti-hyperlipidemia drugs associated with cluster-0 (mid-severity, higher volume). Interestingly, antioxidants vitamin C and E associated with cluster-3 (mid-severity, higher cognition). Anti-depressants like Zoloft associated with most severe disease (cluster-2). Vitamin D is protective for AD, but ARM identified significant underutilization across all AD sub-populations. Identification and feature characterization of four distinct AD sub-population “clusters” using standard clinical features enhances future clinical trial selection criteria and cross-study comparative analysis.
Primary Aldosteronism: Metabolic Reprogramming and the Pathogenesis of Aldosterone-Producing Adenomas
Authors: Gong Siyuan , Tetti Martina , Reincke Martin , Williams Tracy Ann
Published: July 2021

Aldosterone-producing adenomas (APAs) are characterized by aldosterone hypersecretion and deregulated adrenocortical cell growth. Increased energy consumption required to maintain cellular tumorigenic properties triggers metabolic alterations that shape the tumor microenvironment to acquire necessary nutrients, yet our knowledge of this adaptation in APAs is limited. Here, we investigated adrenocortical cell-intrinsic metabolism and the tumor immune microenvironment of APAs and their potential roles in mediating aldosterone production and growth of adrenocortical cells. Using multiple advanced bioinformatics methods, we analyzed gene expression datasets to generate distinct metabolic and immune cell profiles of APAs versus paired adjacent cortex. APAs displayed activation of lipid metabolism, especially fatty acid β-oxidation regulated by PPARα, and glycolysis. We identified an immunosuppressive microenvironment in APAs, with reduced infiltration of CD45+ immune cells compared with adjacent cortex, validated by CD45 immunohistochemistry (3.45-fold, p < 0.001). APAs also displayed an association of lipid metabolism with ferroptosis and upregulation of antioxidant systems. In conclusion, APAs exhibit metabolic reprogramming towards fatty acid β-oxidation and glycolysis. Increased lipid metabolism via PPARα may serve as a key mechanism to modulate lipid peroxidation, a hallmark of regulated cell death by ferroptosis. These findings highlight survival advantages for APA tumor cells with metabolic reprogramming properties.
Combined Simplified Molecular Classification of Gastric Adenocarcinoma, Enhanced by Lymph Node Status: An Integrative Approach
Authors: Daun Till , Nienhold Ronny , Paasinen-Sohns Aino , Frank Angela , Sachs Melanie , Zlobec Inti , Cathomas Gieri
Published: July 2021

Gastric adenocarcinoma (GAC) is a heterogeneous disease and at least two major studies have recently provided a molecular classification for this tumor: The Cancer Genome Atlas (TCGA) and the Asian Cancer Research Group (ARCG). Both classifications quote four molecular subtypes, but these subtypes only partially overlap. In addition, the classifications are based on complex and cost-intensive technologies, which are hardly feasible for everyday practice. Therefore, simplified approaches using immunohistochemistry (IHC), in situ hybridization (ISH) as well as commercially available next generation sequencing (NGS) have been considered for routine use. In the present study, we screened 115 GAC by IHC for p53, MutL Homolog 1 (MLH1) and E-cadherin and performed ISH for Epstein–Barr virus (EBV). In addition, sequencing by NGS for TP53 and tumor associated genes was performed. With this approach, we were able to define five subtypes of GAC: (1) Microsatellite Instable (MSI), (2) EBV-associated, (3) Epithelial Mesenchymal Transition (EMT)-like, (4) p53 aberrant tumors surrogating for chromosomal instability and (5) p53 proficient tumors surrogating for genomics stable cancers. Furthermore, by considering lymph node metastasis in the p53 aberrant GAC, a better prognostic stratification was achieved which finally allowed us to separate the GAC highly significant in a group with poor and good-to-intermediate prognosis, respectively. Our data show that molecular classification of GAC can be achieved by using commercially available assays including IHC, ISH and NGS. Furthermore, we present an integrative workflow, which has the potential to overcome the uncertainty resulting from discrepancies from existing classification schemes.
Reproductive Pattern of Parous Women and the Risk of Cancer in Later Life
Authors: Pasdar Zahra , Scott Neil W. , Iversen Lisa , Hannaford Philip C. , Myint Phyo Kyaw , Bhattacharya Sohinee
Published: July 2021

We assessed the risk of any and site-specific cancers in a case-control study of parous women living in northeast Scotland in relation to: total number of pregnancies, cumulative time pregnant, age at first delivery and interpregnancy interval. We analysed 6430 women with cancer and 6430 age-matched controls. After adjustment for confounders, women with increasing number of pregnancies had similar odds of cancer diagnosis as women with only one pregnancy. The adjusted odds of cancer diagnosis were no higher in women with cumulative pregnancy time 50–150 weeks compared to those pregnant ≤ 50 weeks. Compared with women who had their first delivery at or before 20 years of age, the adjusted odds ratio (AOR) among those aged 21–25 years was 0.81, 95% CI 0.74, 0.88; 26–30 years AOR 0.77, 95% CI 0.69, 0.86; >30 years AOR 0.63, 95% CI 0.55, 0.73. After adjustment, the odds of having any cancer were higher in women who had an inter-pregnancy interval >3 years compared to those with no subsequent pregnancy (AOR 1.17, 95% CI 1.05, 1.30). Older age at first pregnancy was associated with increased risk of breast and gastrointestinal cancer, and reduced risk of invasive cervical, carcinoma in situ of the cervix and respiratory cancer.
Vascular Involvements in Cholangiocarcinoma: Tips and Tricks
Authors: Angelico Roberta , Sensi Bruno , Parente Alessandro , Siragusa Leandro , Gazia Carlo , Tisone Giuseppe , Manzia Tommaso Maria
Published: July 2021

Cholangiocarcinoma (CCA) is an aggressive malignancy of the biliary tract. To date, surgical treatment remains the only hope for definitive cure of CCA patients. Involvement of major vascular structures was traditionally considered a contraindication for resection. Nowadays, selected cases of CCA with vascular involvement can be successfully approached. Intrahepatic CCA often involves the major hepatic veins or the inferior vena cava and might necessitate complete vascular exclusion, in situ hypothermic perfusion, ex situ surgery and reconstruction with autologous, heterologous or synthetic grafts. Hilar CCA more frequently involves the portal vein and hepatic artery. Resection and reconstruction of the portal vein is now considered a relatively safe and beneficial technique, and it is accepted as a standard option either with direct anastomosis or jump grafts. However, hepatic artery resection remains controversial; despite accumulating positive reports, the procedure remains technically challenging with increased rates of morbidity. When arterial reconstruction is not possible, arterio-portal shunting may offer salvage, while sometimes an efficient collateral system could bypass the need for arterial reconstructions. Keys to achieve success are represented by accurate selection of patients in high-volume referral centres, adequate technical skills and eclectic knowledge of the various possibilities for vascular reconstruction.
Identification of an RNA-Binding-Protein-Based Prognostic Model for Ewing Sarcoma
Authors: Chen Yi , Su Huafang , Su Yanhong , Zhang Yifan , Lin Yingbo , Haglund Felix
Published: July 2021

RNA-binding proteins (RBPs) are important transcriptomic regulators and may be important in tumorigenesis. Here, we sought to investigate the clinical impact of RBPs for patients with Ewing sarcoma (ES). ES transcriptome signatures were characterized from four previously published cohorts and grouped into new training and validation cohorts. A total of three distinct subtypes were identified and compared for differences in patient prognosis and RBP signatures. Next, univariate Cox and Lasso regression models were used to identify hub prognosis-related RBPs and construct a prognostic risk model, and prediction capacity was assessed through time-dependent receiver operating characteristics (ROCs), Kaplan–Meier curves, and nomograms. Across the three RBP subtypes, 29 significant prognostic-associated RBP genes were identified, of which 10 were used to build and validate an RBP-associated prognostic risk model (RPRM) that had a stable predictive value and could be considered valuable for clinical risk-stratification of ES. A comparison with immunohistochemistry validation showed a significant association between overall survival and NSUN7 immunoreactivity, which was an independent favorable prognostic marker. The association of RBP signatures with ES clinical prognosis provides a strong rationale for further investigation into RBPs molecular mechanisms.
Gemcitabine Plus Nab-Paclitaxel Induces PD-L1 mRNA Expression in Plasma-Derived Microvesicles in Pancreatic Cancer
Authors: Del Re Marzia , Vivaldi Caterina , Rofi Eleonora , Salani Francesca , Crucitta Stefania , Catanese Silvia , Fontanelli Lorenzo , Massa Valentina , Cucchiara Federico , Fornaro Lorenzo , Capuano Annalisa , Fogli Stefano , Vasile Enrico , Danesi Romano
Published: July 2021

Pancreatic ductal adenocarcinoma (PDAC) is a non-immunogenic tumor poorly responsive to immune checkpoint inhibitors. This study investigates the effect of 5-fluorouracil (5-FU), irinotecan, and oxaliplatin (FOLFIRINOX), and gemcitabine plus nab-paclitaxel (GEMnPAC) regimens on PD-L1 mRNA expression in plasma-derived microvesicles (MVs) in 50 PDAC patients. Plasma was collected before starting chemotherapy and after 3 months of treatment. mRNA was extracted from MVs, and PD-L1 expression was measured by digital droplet PCR. Twenty-eight patients were PD-L1 positive in MVs at baseline, of which 18 were in the GEMnPAC cohort and 10 in the FOLFIRINOX one. The amount of PD-L1 expression in MVs increased from baseline to 3 months of treatment in patients receiving GEMnPAC (median value 0.002 vs. 0.005; p = 0.01) compared to those treated with FOLFIRINOX (median 0.003 vs. 0.004; p = 0.97). The increase in PD-L1 mRNA expression in MVs was not related to tumor response (PR + SD: p = 0.08; PD: p = 0.28). Our findings demonstrate that GEMnPAC can increase PD-L1 mRNA expression in patient-derived circulating MVs, providing a rationale for testing the efficacy of this regimen in sequential or simultaneous combinations with immunotherapy in PDAC patients.
Review of Treatment Options for the Management of Advanced Stage Hodgkin Lymphoma
Authors: Vellemans Hélène , André Marc P. E.
Published: July 2021

Hodgkin lymphoma (HL) is a lymphoid-type hematologic disease that is derived from B cells. The incidence of this lymphoid malignancy is around 2–3/100,000/year in the western world. Long-term remission rates are linked to a risk-adapted approach, which allows remission rates higher than 80%. The first-line treatment for advanced stage classical HL (cHL) widely used today is doxorubicin, bleomycin, vinblastine, and dacarbazine (ABVD) or escalated bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone (BEACOPPesc) chemotherapy. Randomized studies comparing these two regimens and a recently performed meta-analysis have demonstrated consistently better disease control with BEACOPPesc. However, this treatment is not the standard of care, as there is an excess of acute hematological toxicities and therapy-related myeloid neoplasms. Moreover, there is a recurrent controversy concerning the impact on overall survival with this regimen. More recently, new drugs such as brentuximab vedotin and checkpoint inhibitors have become available and have been evaluated in combination with doxorubicin, vinblastine, and dacarbazine (AVD) for the first-line treatment of patients with advanced cHL with the objective of tumor control improvement. There are still major debates with respect to first-line treatment of advanced cHL. The use of positron emission tomography-adapted strategies has allowed a reduction in the toxicity of chemotherapy regimens. Incorporation of new drugs into the treatment algorithms requires confirmation.
Advancing the Measurement of Executive Functioning in Pediatric Chronic Pain
Authors: Beckmann Emily A. , Jastrowski Mano Kristen E.
Published: July 2021

Youth with chronic pain often report executive functioning difficulties, many of which have been linked to poor treatment adherence and health-related quality of life in adults with chronic pain, as well as in other pediatric chronic health populations. Despite the extensive implications for functional impairment, executive functioning remains understudied in pediatric chronic pain. Measurement approaches have lacked clear theoretical guidance, resulting in only some domains of executive functioning being investigated. To date, the methods used to measure executive functioning have been inconsistent, ranging from self-report measures of everyday executive functioning in home and school contexts to standardized neuropsychological tests. We argue for enhanced measure validation efforts and increased clarity in the approaches chosen to measure executive functioning in pediatric chronic pain to better guide research efforts in this area, thus yielding clearer clinical implications.
Comparative Studies of Piston Crown Coating with YSZ and Al2O3·SiO2 on Engine out Responses Using Conventional Diesel and Palm Oil Biodiesel
Authors: Ramasamy Navin , Abul Kalam Mohammad , Varman Mahendra , Teoh Yew Heng
Published: July 2021

In this study, the effect of a thermal barrier coating with yttria-stabilized zirconia (YSZ) and aluminum silicate (Al2O3·SiO2) alongside an NiCrAl bond coat on the engine performance and emission analysis was evaluated by using conventional diesel and pure palm oil biodiesel. These materials were coated on the piston alloy via plasma spray coating. The findings demonstrated that YSZ coating presented better engine performances, in terms of brake thermal efficiency (BTE) and brake-specific fuel consumption (BSFC) for both fuels. The piston with YSZ coating materials achieved the highest BTE (15.94% for diesel, 14.55% for biodiesel) and lowest BSFC (498.96 g/kWh for diesel, 619.81 g/kWh for biodiesel). However, Al2O3·SiO2 coatings indicated better emission with lowest emissions of NO, CO, and CO2 for both diesel and biodiesel. For the uncoated piston, the results indicated that the engine clocked the highest torque and power, especially on diesel fuel due to the high viscosity and low caloric value, and it recorded the lowest hydrocarbon emission due to the complete combustion of fuel in the engine. Hence, it was concluded that the YSZ coating could lead to better engine performance, while Al2O3·SiO2 showed promising results in terms of greenhouse gas emission.
Evolution of High-Quality Homoepitaxial CVD Diamond Films Induced by Methane Concentration
Authors: Zhang Pengfei , Chen Weidong , Zhang Longhui , He Shi , Wang Hongxing , Yan Shufang , Ma Wen , Guo Chunxia , Wang Yanfeng
Published: July 2021

In this paper, we successfully synthesized homoepitaxial diamond with high quality and atomically flat surface by microwave plasma chemical vapor deposition. The sample presents a growth rate of 3 μm/h, the lowest RMS of 0.573 nm, and the narrowest XRD FWHM of 31.32 arcsec. An effect analysis was also applied to discuss the influence of methane concentration on the diamond substrates.
Study of Rechargeable Batteries Using Advanced Spectroscopic and Computational Techniques
Authors: Barbiellini Bernardo , Kuriplach Jan , Saniz Rolando
Published: July 2021

Improving the efficiency and longevity of energy storage systems based on Li- and Na-ion rechargeable batteries presents a major challenge. The main problems are essentially capacity loss and limited cyclability. These effects are due to a hierarchy of factors spanning various length and time scales, interconnected in a complex manner. As a consequence, and in spite of several decades of research, a proper understanding of the ageing process has remained somewhat elusive. In recent years, however, combinations of advanced spectroscopy techniques and first-principles simulations have been applied with success to tackle this problem. In this Special Issue, we are pleased to present a selection of articles that, by precisely applying these methods, unravel key aspects of the reduction–oxidation reaction and intercalation processes. Furthermore, the approaches presented provide improvements to standard diagnostic and characterisation techniques, enabling the detection of possible Li-ion flow bottlenecks causing the degradation of capacity and cyclability.