bluuebunny/crossref_metadata_2025 huggingface dataset:
11. Conclusion Making Change
Authors: Hirst Aggie , de Merich Diego , Hoover Joe , Roccu Roberto
Published: August 2023

This chapter highlights the importance of critical thinking as a vital tool in bringing about change in global politics. It explains how critical thinking allows people to make informed and well-reasoned decisions in situations such as jobs, voting, and being either right or wrong. Voting and the Covid-19 pandemic are some examples of everyday experiences affected by global politics, power relations, and political relationships. The chapter also clarifies how myth often recounts historical events in terms of patterned signs, symbols, or meanings instead of narrating as factual chronology. Moreover, it introduces Nesrine Malik’s four tools for thinking differently in and about global politics, and Karen Armstrong’s analysis of mythology that could assist in imagining new and beneficial political ideas and arrangements.
7. Production and business
Authors: Macdonald Kate
Published: August 2023

This chapter addresses global production, which has powerful effects on the incomes, working conditions, and development opportunities of populations around the world. It is not surprising that the organization and regulation of global production have become one of the most contentious subjects of debate within the field of Global Political Economy (GPE). The chapter confronts several difficult questions linked to these debates. Who does the work of global production, and how has its organization changed over time? Who exercises power within evolving systems of global production, and what winners and losers do such arrangements produce? How is global production governed, and with what consequences for labour rights and the environment? In exploring these questions, analytical lenses drawn from a range of political economy perspectives help us to make sense of the complex economic and political forces through which the organization and governance of contemporary global production is shaped and contested.
8. Health
Authors: Rushton Simon
Published: August 2023

This chapter describes a wide range of contemporary health challenges. It begins by assessing what it means to be healthy. The Covid-19 pandemic, and the response to it, have brought to the fore and shed new light on many of the issues that are core to Global Political Economy (GPE). Despite spectacular advances, there are huge inequalities in health in the world today, both within and between countries. Improving health requires both prevention and cure: public health efforts to protect and promote the health of populations, and healthcare services that are accessible to all in times of need. The chapter then considers how the social, economic, and commercial determinants of health can best be understood by adopting a GPE lens. A GPE framework can also reveal the challenges the world faces in its attempt to achieve universal access to quality healthcare.
10. Inequality
Authors: Ortiz-Juarez Eduardo , Sumner Andy
Published: August 2023

This chapter explores income inequality in the global political economy. Income inequality matters for intrinsic and instrumental reasons, and intersects with inequalities between social groups based on gender, race, and other factors. There are three ways to think about income inequality at a global level: ‘international inequality’, ‘world inequality’, and ‘global inequality’. One can say that international inequality and world inequality have unambiguously declined since 1980. However, the magnitude of the decline depends on whether the size of countries' populations is taken into account. Meanwhile, national inequality refers to differences in income between individuals within a country. The chapter then discusses poverty. Ultimately, explanations for patterns of inequality in the contemporary period can be traced to many of the dynamics associated with globalization, particularly the reorganization of the global economy around global value chains (GVCs) and the implications for countries pursuing ‘late development’.
12. Labour and work
Authors: Alford Matthew
Published: August 2023

This chapter illustrates the complex and contested relationship between global production and labour. The mode of global production has changed dramatically since the 1970s. Since the 1990s, corporations have outsourced the production of goods to suppliers around the world. At the core of this contemporary form of global production is the ability of lead firms to profit through advanced sourcing strategies, economies of scale, and branding. This gives corporations significant bargaining power over their fragmented and geographically dispersed supplier base. In the contemporary global economy, conditions of poverty and marginalization can be attributed not only to exclusion from employment, but also to the adverse incorporation of precarious workers into global production. The chapter then considers the role of national governments in the governance of labour in global production, before looking at the impact of e-commerce and automation on the future of work.
13. Health
Authors: Davies Sara E.
Published: August 2023

This chapter describes the increasingly prominent representation of health as a security issue. It begins by presenting the ‘origin’ story of health security that has led to the contemporary practices we see today in the WHO and UN Security Council. The chapter then looks at the different approaches to health security—namely, human security and national security—and considers why security is mobilized to respond to health issues. The focus here is on public health events and their location (regions and borders). The chapter also examines who the ‘peoples’ to be protected from the dangers of health security are. The COVID-19 pandemic reveals that despite a rapidly emerging global public health threat endangering everyone, with some more exposed to harm than others, the response was not equitable and reinforced existing hierarchies.
19. Borders and mobility
Authors: Muller Benjamin J.
Published: August 2023

This chapter reviews the security politics of borders and mobility. Borders and bordering practices—such as surveillance, ID technology, and the virtual border—are presented as essential to security. Conversely, forms of mobility are framed as threats, and in the process mobile actors are regularly rendered precarious and dehumanized. How should we understand borders as spaces of contestation? To what extent are borders and mobility about the production and negotiation of belonging and precarity, sometimes violently, along hierarchies of class, gender, race, sexuality, indigeneity, and territoriality? The chapter focuses on territorial sovereign borders and demonstrates the extent to which borders, bordering practices, and boundary making are framed as ‘belonging’ and mobility well beyond territoriality.
Genomic basis of selective breeding from the closest wild relative of large-fruited tomato
Authors: Yang Junwei , Liu Yun , Liang Bin , Yang Qinqin , Li Xuecheng , Chen Jiacai , Li Hongwei , Lyu Yaqing , Lin Tao
Published: August 2023

The long and intricate domestication history of the tomato (Solanum lycopersicum) includes selection sweeps that have not been fully explored, and these sweeps show significant evolutionary trajectories of domestication traits. Using three distinct selection strategies, we represented comprehensive selected sweeps from 53 Solanum pimpinellifolium (PIM) and 166 S. lycopersicum (BIG) accessions, which are defined as pseudo-domestication in this study. We identified 390 potential selection sweeps, some of which had a significant impact on fruit-related traits and were crucial to the pseudo-domestication process. During tomato pseudo-domestication, we discovered a minor–effect allele of the SlLEA gene related to fruit weight (FW), as well as the major haplotypes of fw2.2/cell number regulator (CNR), fw3.2/SlKLUH, and fw11.3/cell size regulator (CSR) in cultivars. Furthermore, 18 loci were found to be significantly associated with FW and six fruit-related agronomic traits in genome-wide association studies. By examining population differentiation, we identified the causative variation underlying the divergence of fruit flavonoids across the large-fruited tomatoes and validated BRI1-EMS-SUPPRESSOR 1.2 (SlBES1.2), a gene that may affect flavonoid content by modulating the MYB12 expression profile. Our results provide new research routes for the genetic basis of fruit traits and excellent genomic resources for tomato genomics-assisted breeding.
Effects of dietary protein content and crystalline amino acid supplementation patterns in low protein diets on intestinal bacteria and their metabolites in weaned pigs raised under Different sanitary conditions
Authors: Lee Jinyoung , Htoo John K , Kluenemann Martina , González-Vega J Caroline , Nyachoti Charles Martin
Published: August 2023

The objective of this experiment was to investigate the effects of dietary crude protein (CP) content and crystalline amino acids (CAA) supplementation patterns in low CP (LCP) diets on intestinal bacteria and their metabolites in weaned pigs raised under clean (CSC) or unclean sanitary conditions (USC). One hundred forty-four piglets (6.35 ± 0.63 kg) were assigned to one of six treatments in a 3 × 2 factorial arrangement based on CP content and sanitary conditions in a randomized complete block design to give eight replicates with three pigs per pen over a 21-d period. Diets consisted of a high CP (HCP; 21%) and two LCP (18%) diets supplemented with 9 CAA (Lys, Met, Thr, Trp, Val, Ile, Leu, His, and Phe) or only six CAA (Lys, Met, Thr, Trp, Val, and Ile) to meet the requirements. The CSC room was washed weekly, whereas the USC room had sow manure spread in the pens from the beginning of the study and was not washed throughout the experiment. Jejunum and colon digesta were sampled on day 21. Both jejunum and colon digesta were analyzed for ammonia nitrogen, short-chain fatty acids, and biogenic amines but only colon digesta was analyzed for microbiome composition (16s rRNA sequencing on MiSeq). Data were analyzed using R software for 16S rRNA and the MIXED procedure of SAS for microbial metabolites. Sanitation, CP content, and CAA supplementation patterns did not affect the diversity of colonic bacterial composition in weaned pigs. Pigs raised under USC had greater (P < 0.05) jejunal ammonia nitrogen concentration than those raised under CSC. Pigs fed LCP diets had reduced (P < 0.05) jejunal ammonia nitrogen concentration compared to those fed the HCP diet. Interactions between sanitation and dietary CP content were observed (P < 0.05) for: (1) jejunal acetate and (2) colonic spermidine and spermine, whereby (1) acetate concentrations decreased from NCP to LCP in pigs raised under the CSC but those concentrations increased under the USC, and (2) spermidine and spermine concentrations increased in LCP diets compared to HCP diet under USC, unlike CSC which did not show any difference between HCP and LCP. In conclusion, reducing dietary CP lowered ammonia nitrogen content regardless of sanitation and increased microbial metabolites in weaned pigs raised under USC. However, LCP diets with different CAA supplementation patterns did not affect bacterial diversity in weaned pigs, regardless of the hygienic conditions where the animals were housed.
The effects of timing of high immune response phenotyping in relation to weaning on immune responses of crossbred beef calves
Authors: Beard Shannon C , Schmied Julie D , Hodgins Douglas C , Mallard Bonnie A
Published: August 2023

Genetic selection for immune response has the potential to increase the sustainability of the beef industry by breeding cattle that are productive yet with an increased capacity to resist disease. Determining the optimal time to immunophenotype beef cattle is crucial for the accurate prediction of an animal’s immune response. The objective of this study was to determine the effect of time of immunophenotyping in relation to weaning on immune responses of beef calves. Antibody- (AMIR) and cell-mediated (CMIR) immune responses were measured on 97 calves on the day of weaning (WEANING, N = 56) or 2 mo post-weaning (POST-WEANING, N = 41). Within each period of immunophenotyping, on day 0, blood was collected, and calves received a 1.0 mL intramuscular injection of type 1 and 2 test antigens. On day 14, blood was collected, and baseline skinfold thickness (SFT) was measured. Calves received an intradermal injection of 0.1 mg of the type 1 antigen suspended in 0.1 mL phosphate buffered saline (PBS) in the right tail fold, and 0.1 mL of PBS in the left. Changes in SFT at 24 h was used to indicate CMIR. To assess AMIR, the titer of type 2 antigen-specific bovine immunoglobulin G in serum from blood collected on day 14 was determined by measuring optical density (OD) using an enzyme-linked immunosorbent assay (ELISA). Among heifers, AMIR was greater for the POST-WEANING group than for the WEANING group (P < 0.01). Among steers, AMIR was not different between the POST-WEANING group and the WEANING group (P = 1.0). Therefore, the AMIR of heifers may be more negatively affected by immunophenotyping at weaning than the AMIR of steers. For steers, CMIR was greater in the POST-WEANING group than the WEANING group (P < 0.001). For heifers, CMIR was not different between the POST-WEANING group and the WEANING group (P = 0.22). The CMIR of steers may be more negatively affected by immunophenotyping at weaning than the CMIR of heifers. Calf age was not associated with AMIR or CMIR for calves phenotyped at weaning or post-weaning. The effect of sire nested within dam age was significant for CMIR for calves in the POST-WEANING group (P < 0.01), but not for calves in the WEANING group (P = 0.67). The results suggest that measuring immunocompetence at weaning may not be representative of a calf’s genetic ability to mount an effective immune response, and immunophenotyping should be performed outside the weaning period.
Benchmarking global biodiversity of decapod crustaceans (Crustacea: Decapoda)
Authors: De Grave Sammy , Decock Wim , Dekeyzer Stefanie , Davie Peter J F , Fransen Charles H J M , Boyko Christopher B , Poore Gary C B , Macpherson Enrique , Ahyong Shane T , Crandall Keith A , de Mazancourt Valentin , Osawa Masayuki , Chan Tin-Yam , Ng Peter K L , Lemaitre Rafael , van der Meij Sancia E T , Santos Sandro
Published: August 2023

A new assessment of the global biodiversity of decapod Crustacea (to 31 December 2022) records 17,229 species in 2,550 genera and 203 families. These figures are derived from a well-curated dataset maintained on the online platform DecaNet, a subsidiary of the World Register of Marine Species (WoRMS). Distinct phases are recognised in the discovery process (as measured by species descriptions) corresponding to major historical and geopolitical time periods, with the current rate of species descriptions being more than three times higher than in the Victorian age of global exploration. Future trends are briefly explored, and it is recognised that a large number of species remain to be discovered and described.
The effect of sunflower pollen age and origin on pathogen infection in the common eastern bumble bee (Apidae: Hymenoptera)
Authors: Fowler Alison E , Kola Elisa , Adler Lynn S
Published: August 2023

Bumble bees are globally important pollinators, contributing hundreds of millions of dollars annually in crop pollination services. Several species are in decline, making it paramount to understand how pathogens and nutrition shape bee health. Previous work has shown that consuming sunflower pollen (Helianthus annuus) dramatically reduces infection by the trypanosomatid gut pathogen, Crithidia bombi, in the common eastern bumble bee (Bombus impatiens). Sunflower pollen may therefore be useful as a dietary supplement for reducing this pathogen in managed bumble bee colonies. Here, we assessed the efficacy of freezer-stored sunflower pollen that was collected in different years and locations for reducing pathogen infection. We tested sunflower pollen that was 1, 3, 4, or 5 yr old and from sunflowers grown in the United States or China against a control of 1-yr-old buckwheat pollen from China, since buckwheat pollen results in high infection. We hypothesized that older pollen would have weaker medicinal effects due to degradation of pollen quality. We found that all sunflower pollen treatments significantly decreased Crithidia infection compared to controls. These results suggest that sunflower pollen can be freezer-stored for up to 5 yr and sourced from a wide range of geographic areas and still maintain its medicinal effects against Crithidia in the common eastern bumble bee. This is helpful information for stakeholders who might administer sunflower pollen as a dietary supplement to manage Crithidia in commercial bumble bee colonies.
Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
Authors: Rana Seema , Kang Changsun , Allred Julianne , Medina-Duran Jorge Humberto , Canova Alyssa , Sherry Danielle , Woller Derek A , Kim Dongin , Song Hojun
Published: August 2023

The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states of the western United States. During the past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to the rangeland forage and agricultural crops, particularly grain crops. Current population management methods rely heavily on broad-spectrum chemical insecticides, which could be toxic to nontargets, and even the targeted species might develop resistance in the long run. Therefore, we assessed the potential of RNA interference (RNAi)-based alternative management strategies that could supplement the current methods. In insects, RNAi efficiency varies with the method of double-stranded RNA (dsRNA) delivery. We tested 2 different methods of dsRNA delivery: injection and oral feeding of dsRNA. The results showed that Mormon crickets are sensitive to injection of dsRNA in a dose-dependent manner, but refractory to the oral feeding of dsRNA. Further, we confirmed the high nuclease activity in the insect midgut. In order to protect the dsRNA from the dsRNase activity and facilitate its uptake in the midgut, we encapsulated dsRNA inside poly lactic-co-glycolic acid (PLGA) nanoparticles and studied its release kinetics and RNAi efficiency by oral feeding. The release kinetics clearly suggested that the PLGA nanoparticle permeates from the insect digestive system to the hemolymph; however, it failed to induce an efficient RNAi response of the targeted genes. In conclusion, our findings suggest the different responses to dsRNA delivery methods in Mormon crickets, and further investigations involving dsRNA stability and its uptake mechanism are required to use RNAi as an alternative Mormon cricket population management strategy.
Trends and cancer-specific patterns of physical activity, sleep duration, and daily sitting time among US cancer survivors, 1997-2018
Authors: Cao Chao , Patel Alpa V , Liu Raymond , Cao Yin , Friedenreich Christine M , Yang Lin
Published: August 2023

Background

Physical activity, sufficient sleep, and limiting sedentary time may improve cancer survivorship.

Methods

Utilizing US nationally representative samples from the National Health Interview Survey 1997-2018 and the National Health and Nutrition Examination Survey 2007-2018, this study investigated the trends of meeting physical activity guidelines, insufficient sleep duration, and sitting time in US cancer survivors (n = 58 527) and noncancer adults (n = 640 109).

Results

From 1997 to 2018, the prevalence of meeting physical activity guidelines was consistently lower in cancer survivors than in noncancer adults. Among cancer survivors, the prevalence of meeting physical activity guidelines increased from 34.9% (95% confidence interval [CI] = 33.1% to 36.8%) to 46.5% (95% CI = 45.0% to 48.1%) for aerobic (≥150 minutes per week at moderate intensity or 75 minutes per week at vigorous intensity), from 13.9% (95% CI = 12.8% to 15.1%) to 23.1% (95% CI = 21.8% to 24.4%) for muscle strengthening (≥2 days per week) activities, and from 9.5% (95% CI = 8.4% to 10.7%) to 17.9% (95% CI = 16.7% to 19.1%) for both combined (all Ptrend < .001). From 2004 to 2018, the prevalence of insufficient sleep duration (<7 hours per day) increased from 28.4% (95% CI = 26.3% to 30.5%) to 30.8% (95% CI = 29.3% to 32.2%) (Ptrend = .004). Daily sitting time increased from 6.09 hours per day (95% CI = 5.71 to 6.46 hours per day) in 2007-2008 to 7.36 hours per day (95% CI = 7.05 to 7.68 hours per day) in 2013-2014 and attenuated to 6.20 hours per day (95% CI = 5.74 to 6.65 hours per day) in 2017-2018. The pattern of physical activity, sleep, and sitting time varied by sex, race and ethnicity, body mass index, cancer type, and time since cancer diagnosis.

Conclusions

More than half of US cancer survivors did not meet physical activity guidelines, and a large proportion had insufficient sleep duration and prolonged sitting time.
Hot, hotter, hottest?
Authors: Prats Jordi
Published: August 2023

Extreme value theory (EVT) uses the historical record to predict which parts of the world can expect exceptional heatwaves, so that policy-makers can try to mitigate the impact. But as statistically implausible heatwaves hit countries across the world, asks Jordi Prats, can EVT still be relied on to keep people safe?
Mining of a novel reductase and its application for asymmetric reduction of p-methoxyacetophenone
Authors: Wang Nengqiang , Li Xiaojun
Published: August 2023

(R)-1-(4-methoxyphenyl) ethanol [(R)-1b] is an essential precursor for the synthesis of aryl propanoic acids’ anti-inflammatatory drugs. Biocatalysts for (R)-1b preparation are limited and reductase has problems of low substrate concentration and low conversion rate. As a result, there is a constant need for discovering novel biocatalysts with excellent catalytic performances. In this study, a novel reductase LpSDR from Lacisediminihabitans profunda for the biocatalytic reduction of p-methoxyacetophenone (1a) to (R)-1b was obtained based on gene-mining technology, and some key reaction parameters were also investigated to improve the conversion rate of 1a using whole cells of recombinant Escherichia coli expressing reductase LpSDR as biocatalysts. It was found that the optimal concentration of isopropanol, ZnSO4·7H2O solution, 1a, and recombinant E. coli resting cells, the optimal reaction temperature, buffer pH, and reaction time were 1.95 mol l−1, 0.75 mmol l−1, 75 mmol l−1, 250 g (wet weight) l−1, 28°C, 7.0, and 21 h, respectively. Under the above conditions, a conversion rate of 99.5% and an enantiomeric excess of 99.6% were obtained, which were superior to the corresponding values previously reported. This study provides a novel reductase LpSDR, which is helpful in reducing 1a to (R)-1b.
Further evidence of the link between activity and metallicity using the flaring properties of stars in the Kepler field
Authors: See Victor , Roquette Julia , Amard Louis , Matt Sean
Published: August 2023

The magnetic activity level of low-mass stars is known to vary as a function of the physical properties of the star. Many studies have shown that the stellar mass and rotation are both important parameters that determine magnetic activity levels. In contrast, the impact of a star’s chemical composition on magnetic activity has received comparatively little attention. Data sets for traditional activity proxies, e.g. X-ray emission or calcium emission, are not large enough to search for metallicity trends in a statistically meaningful way. Recently, studies have used the photometric variability amplitude as a proxy for magnetic activity to investigate the role of metallicity because it can be relatively easily measured for large samples of stars. These studies find that magnetic activity and metallicity are positively correlated. In this work, we investigate the link between activity and metallicity further by studying the flaring properties of stars in the Kepler field. Similar to the photometric variability, we find that flaring activity is stronger in more metal-rich stars for a fixed mass and rotation period. This result adds to a growing body of evidence that magnetic field generation is correlated with metallicity.
Backsplash galaxies and their impact on galaxy evolution: a three-stage, four-type perspective
Authors: Ruiz Andrés N , Martínez Héctor J , Coenda Valeria , Muriel Hernán , Cora Sofía A , de los Rios Martín , Vega-Martínez Cristian A
Published: August 2023

We study the population of backsplash galaxies at z = 0 in the outskirts of massive, isolated clusters of galaxies taken from the mdpl2-sag semi-analytical catalogue. We consider four types of backsplash galaxies according to whether they are forming stars or passive at three stages in their lifetimes: before entering the cluster, during their first incursion through the cluster, and after they exit the cluster. We analyse several geometric, dynamic, and astrophysical aspects of the four types at the three stages. Galaxies that form stars at all stages account for the majority of the backsplash population (58 per cent) and have stellar masses typically below $M_\star \sim 3\times 10^{10} \ h^{-1}\, {\rm {M_\odot }}$ that avoid the innermost cluster’s regions and are only mildly affected by it. In a similar mass range, galaxies that become passive after exiting the cluster (26 per cent) follow orbits characterized by small pericentric distance and a strong deflection by the cluster potential well while suffering a strong loss of both dark matter and gas content. Only a small fraction of our sample (4 per cent) becomes passive while orbiting inside the cluster. These galaxies have experienced heavy pre-processing and the cluster’s tidal stripping and ram pressure provide the final blow to their star formation. Finally, galaxies that are passive before entering the cluster for the first time (12 per cent) are typically massive and are not affected significantly by the cluster. Using the bulge/total mass ratio as a proxy for morphology, we find that a single incursion through a cluster does not result in significant morphological changes in all four types.
A self-consistent semi-analytic model for Population III star formation in minihaloes
Authors: Hegde Sahil , Furlanetto Steven R
Published: August 2023

The formation of the first stars marks a watershed moment in the history of our Universe. As the first luminous structures, these stars (also known as Population III, or Pop III stars) seed the first galaxies and begin the process of reionization. We construct an analytic model to self-consistently trace the formation of Pop III stars inside minihaloes in the presence of the fluctuating ultraviolet background, relic dark matter (DM)-baryon relative velocities from the early universe, and an X-ray background, which largely work to suppress cooling of gas and delay the formation of this first generation of stars. We demonstrate the utility of this framework in a semi-analytic model for early star formation that also follows the transition between Pop III and Pop II star formation inside these haloes. Using our new prescription for the criteria allowing Pop III star formation, we follow a population of DM haloes from z = 50 through z = 6 and examine the global star formation history, finding that each process defines its own key epoch: (i) the stream velocity dominates at the highest redshifts (z ≳ 30), (ii) the UV background sets the tone at intermediate times (30 ≳ z ≳ 15), and (iii) X-rays control the end of Pop III star formation at the latest times (z ≲ 15). In all of our models, Pop III stars continue to form down to z ∼ 7–10, when their supernovae will be potentially observable with forthcoming instruments. Finally, we identify the signatures of variations in the Pop III physics in the global 21-cm spin–flip signal of atomic hydrogen.
Numerical simulations of the random angular momentum in convection – II. Delayed explosions of red supergiants following ‘failed’ supernovae
Authors: Antoni Andrea , Quataert Eliot
Published: August 2023

When collapse of the iron core in a massive red or yellow supergiant does not lead to an energetic supernova, a significant fraction of the convective hydrogen envelope will fall in towards the black hole formed from the collapsing core. The random velocity field in the convective envelope results in finite specific angular momentum in each infalling shell. Using 3D hydrodynamical simulations, we follow the infall of this material to small radii, resolving the circularization radii of the flow. We show that infall of the convective envelope leads to nearly complete envelope ejection in a ≳1048 erg explosion with outflow speeds of ≳200 km s−1. The light curve of such an explosion would show a characteristic, red plateau as the ejecta cools and a hydrogen recombination front recedes through the expanding ejecta. Adopting supernova IIp scalings, the event would have a plateau luminosity of ≳1040 erg s−1 and a duration of several hundreds of days. These events would appear quite similar to luminous red novae with red or yellow supergiant progenitors; some luminous red novae may, in fact, be signposts of black hole formation. The mechanism studied here produces more energetic explosions than the weak shock generated from radiation of neutrino energy during the protoneutron star phase. Because we cannot simulate all the way to the horizon, our results are likely lower limits on the energy and luminosity of transients produced during the collapse of a red or yellow supergiant to form a black hole.
Germ Granule Evolution Provides Mechanistic Insight into Drosophila Germline Development
Authors: Doyle Dominique A , Burian Florencia N , Aharoni Benjamin , Klinder Annabelle J , Menzel Melissa M , Nifras Gerard Carlo C , Shabazz-Henry Ahad L , Palma Bianca Ulrich , Hidalgo Gisselle A , Sottolano Christopher J , Ortega Bianca M , Niepielko Matthew G
Published: August 2023

The copackaging of mRNAs into biomolecular condensates called germ granules is a conserved strategy to posttranscriptionally regulate germline mRNAs. In Drosophila melanogaster, mRNAs accumulate in germ granules by forming homotypic clusters, aggregates containing multiple transcripts from the same gene. Nucleated by Oskar (Osk), homotypic clusters are generated through a stochastic seeding and self-recruitment process that requires the 3′ untranslated region (UTR) of germ granule mRNAs. Interestingly, the 3′ UTR belonging to germ granule mRNAs, such as nanos (nos), have considerable sequence variations among Drosophila species and we hypothesized that this diversity influences homotypic clustering. To test our hypothesis, we investigated the homotypic clustering of nos and polar granule component (pgc) in four Drosophila species and concluded that clustering is a conserved process used to enrich germ granule mRNAs. However, we discovered germ granule phenotypes that included significant changes in the abundance of transcripts present in species’ homotypic clusters, which also reflected diversity in the number of coalesced primordial germ cells within their embryonic gonads. By integrating biological data with computational modeling, we found that multiple mechanisms underlie naturally occurring germ granule diversity, including changes in nos, pgc, osk levels and/or homotypic clustering efficacy. Furthermore, we demonstrated how the nos 3′ UTR from different species influences nos clustering, causing granules to have ∼70% less nos and increasing the presence of defective primordial germ cells. Our results highlight the impact that evolution has on germ granules, which should provide broader insight into processes that modify compositions and activities of other classes of biomolecular condensate.
Identification of Sociodemographic, Clinical, and Genetic Factors to Aid Alaska Native and American Indian People to Successfully Quit Smoking
Authors: Avey Jaedon P , Schaefer Krista R , Noonan Carolyn J , Trinidad Susan B , Muller Clemma J , Claw Katrina G , Dillard Denise A , Todd Michael R , Beans Julie A , Tyndale Rachel F , Robinson Renee F , Thummel Kenneth E
Published: August 2023

Introduction

Alaska Native and American Indian (ANAI) people have a smoking prevalence of 23%. Nicotine metabolite ratio (NMR) and genetic testing may enable tailored selection of tobacco cessation medication.

Aims and Methods

The purpose of this study was to evaluate the relative contributions of NMR, cessation medication, demographics, and tobacco use history to cessation. Participants were recruited into an observational cohort study consisting of a baseline visit prior to their quit date and 6-week follow-up. Demographic and tobacco use surveys and blood, urine, and breath samples were collected at each visit. Electronic health records were queried for cessation medications. NMR was categorized into slow or normal nicotine metabolism phenotypes (<0.31 and ≥ 0.31, respectively). The main outcome was cessation at 6 weeks. Analyses consisted of descriptive statistics, medication and phenotype concordance, and estimates of relative risk (RR) of quitting.

Results

We enrolled 151 ANAI adults who smoked cigarettes daily. Two-thirds had normal nicotine metabolism phenotype. Retrospective medication and phenotype concordance was 39%. The overall quit rate was 25%. No demographic factors or tobacco use history were associated with quit success. Varenicline and bupropion increased the likelihood of quitting (RR = 2.93 [1.42, 6.03] and RR = 2.52 [1.12, 5.64], respectively) compared to nicotine replacement therapy. Non-optimal medication and phenotype concordance decreased likelihood of quit success (RR = 0.44 [0.22, 0.91]) compared to optimal concordance.

Conclusions

This exploratory study found associations between quit success and tobacco cessation medication as well as medication and phenotype concordance. Additional research is needed to assess use of NMR for treatment selection among ANAI people.

Implications

These results broadly support additional community-engaged research to improve medication and phenotype concordance in tribal health settings. Such future research on implementing meditcation and phenotype concordance holds promise to improve expectations, quit success, and health outcomes amongst individuals attempting to quit smoking.
Corticosteroid Therapy for Patients With Severe Fever With Thrombocytopenia Syndrome: A Nationwide Propensity Score–Matched Study in Japan
Authors: Shuto Hisayuki , Komiya Kosaku , Usagawa Yuko , Yamasue Mari , Fushimi Kiyohide , Hiramatsu Kazufumi , Kadota Jun-ichi
Published: August 2023

Background

Severe fever with thrombocytopenia syndrome (SFTS) is a life-threatening infectious disease for which no effective treatment strategy has been established. Although corticosteroids (CSs) are widely administered to patients with SFTS, their efficacy remains uncertain. This study aimed to assess the impact of CS therapy on the in-hospital mortality of patients with SFTS.

Methods

In this nationwide observational study using the Japanese Diagnosis Procedure Combination database, patients hospitalized for SFTS from April 2013 to March 2021 were reviewed. We compared patients who were treated with CSs to those who were treated without them after propensity score matching to adjust for their background, disease severity, and combination therapy.

Results

We included 494 patients with SFTS, and 144 pairs of them were analyzed after propensity score matching. No significant difference in the 30-day mortality (19% vs 15%, P = .272) and the number of survival days (log-rank test, P = .392) was found between the CS treatment group and the non-CS treatment group. However, in subgroup analyses, the CS treatment group tended to have better survival among patients with impaired consciousness on admission and/or shock status within 7 days after admission.

Conclusions

CS therapy does not seem effective for all patients with SFTS; however, the impact might be altered by disease severity assessed by the consciousness level and shock status. A large-scale interventional study is required to determine its efficacy, especially for critically ill patients with SFTS.
COVID-19 and social media: Beyond polarization
Authors: De Nicola Giacomo , Tuekam Mambou Victor H , Kauermann Göran
Published: August 2023

The COVID-19 pandemic brought upon a massive wave of disinformation, exacerbating polarization in the increasingly divided landscape of online discourse. In this context, popular social media users play a major role, as they have the ability to broadcast messages to large audiences and influence public opinion. In this article, we make use of openly available data to study the behavior of popular users discussing the pandemic on Twitter. We tackle the issue from a network perspective, considering users as nodes and following relationships as directed edges. The resulting network structure is modeled by embedding the actors in a latent social space, where users closer to one another have a higher probability of following each other. The results suggest the existence of two distinct communities, which can be interpreted as “generally pro” and “generally against” vaccine mandates, corroborating existing evidence on the pervasiveness of echo chambers on the platform. By focusing on a number of notable users, such as politicians, activists, and news outlets, we further show that the two groups are not entirely homogeneous, and that not just the two poles are represented. To the contrary, the latent space captures an entire spectrum of beliefs between the two extremes, demonstrating that polarization, while present, is not the only driver of the network, and that more moderate, “central” users are key players in the discussion.
Short-wave infrared fluorescence imaging of near-infrared dyes with robust end-tail emission using a small-animal imaging device
Authors: Arena Francesca , La Cava Francesca , Faletto Daniele , Roberto Miriam , Crivellin Federico , Stummo Francesco , Adamo Alessia , Boccalon Mariangela , Napolitano Roberta , Blasi Francesco , Koch Maximilian , Taruttis Adrian , Reitano Erika
Published: August 2023

Commercially available near-infrared (NIR) dyes, including indocyanine green (ICG), display an end-tail of the fluorescence emission spectrum detectable in the short-wave infrared (SWIR) window. Imaging methods based on the second NIR spectral region (1,000–1,700 nm) are gaining interest within the biomedical imaging community due to minimal autofluorescence and scattering, allowing higher spatial resolution and depth sensitivity. Using a SWIR fluorescence imaging device, the properties of ICG vs. heptamethine cyanine dyes with emission >800 nm were evaluated using tissue-simulating phantoms and animal experiments. In this study, we tested the hypothesis that an increased rigidity of the heptamethine chain may increase the SWIR imaging performance due to the bathochromic shift of the emission spectrum. Fluorescence SWIR imaging of capillary plastic tubes filled with dyes was followed by experiments on healthy animals in which a time series of fluorescence hindlimb images were analyzed. Our findings suggest that higher spatial resolution can be achieved even at greater depths (>5 mm) or longer wavelengths (>1,100 nm), in both tissue phantoms and animals, opening the possibility to translate the SWIR prototype toward clinical application.
Non-invasive imaging and clinical skin scores in juvenile localized scleroderma
Authors: Pain Clare E , Murray Andrea , Dinsdale Graham , Marsden Antonia , Manning Joanne , Riley Phil , Leone Valentina , Amin Tania , Zulian Francesco , Herrick Ariane L
Published: August 2023

Objectives

To evaluate whether in juvenile localized scleroderma (JLS), non-invasive imaging can differentiate affected from non-affected skin and whether imaging correlates with a validated skin score [Localised Scleroderma Cutaneous Assessment Tool (LoSCAT)].

Methods

A total of 25 children with JLS were recruited into a prospective study and a single ‘target’ lesion was selected. High-frequency ultrasound (HFUS, measuring skin thickness), infrared thermography (IRT, skin temperature), laser Doppler imaging (LDI, skin blood flow) and multispectral imaging (MSI, oxygenation) were performed at four sites: two of affected skin (centre and inner edge of lesion) and two of non-affected skin (1 cm from the edge of the lesion ‘outer’ and contralateral non-affected side) at four visits at 3 month intervals.

Results

Differences between affected and non-affected skin were detected with all four techniques. Compared with non-affected skin, affected skin was thinner (P < 0.001), with higher temperature (P < 0.001–0.006), perfusion (P < 0.001–0.039) and oxygenation (P < 0.001–0.028). Lesion skin activity (LoSCAT) was positively correlated with centre HFUS [r = 0.32 (95% CI 0.02, 0.61), P = 0.036] and negatively correlated with centre LDI [r = −0.26 (95% CI −0.49, −0.04), P = 0.022]. Lesion skin damage was positively correlated with centre and inner IRT [r = 0.43 (95% CI 0.19, 0.67), P < 0.001 and r = 0.36 (95% CI 0.12, 0.59), P = 0.003, respectively] and with centre and inner LDI [r = 0.37 (95% CI 0.05, 0.69), P = 0.024 and r = 0.41 (95% CI 0.08, 0.74), P = 0.015, respectively].

Conclusion

Non-invasive imaging can detect differences between affected and non-affected skin in JLS and may help to differentiate between activity (thicker, less well-perfused skin) and damage (thinner, highly perfused skin).
Cedrus deodara (Roxb.): a review on the recent update on its pharmacological and phytochemical profile
Authors: Pathak Harsh , Pathania Sakshi , Metha Shradha , Sharma Ramica
Published: August 2023

Objectives

Cedrus deodara (Roxb.) Loud, commonly called as deodar, belongs to the family Pinaceae and is a species of cedar native to the Western Himalayas in Eastern Afghanistan, Northern Pakistan, North-Central India, South Western Tibet and Western Nepal. The current review summarized and presents an up-to-date article on the medicinal uses, phytochemistry and biological activities of C. deodara plant.

Key findings

It is a medicinal tree and is used traditionally to treat various diseases like microbial infection, joint disorder, asthma, skin diseases, kidney stone, peptic ulcer, brain disorders and inflammatory disorders. The chemical constituents obtained from different parts of the plant include α-himachalene (12.5%) and β-himachalene (43%) associated with them are sesquiterpene alcohols (himachalol, allohimachalol, himadarol, isocentdarol, centdarol and cedrin (6-methyldihydromyricetin), methylacetophenone, atlantonl, deodarin, toxifolin, terpenoids, flavonoids and glycosides, wikstromal, matairesinol, dibenzylbutyrolactol, bergapten, isopimpinellin, benzofuranoid neo lignan and isohemacholone.

Its chemical constituents are mostly responsible for the pharmacological actions. Recent in-vivo and in-vitro studies show that C. deodara has anti-inflammatory, analgesic, anti-hyperglycaemic, anti-spasmodic, insecticidal, anti-apoptotic, anti-cancer, immmunomodulatory, molluscidal, anxiolytic and anticonvulsant properties. However, immediate efforts must be made to determine its mode of action, efficacy, dose range and safety in addressing various disease situations.
Populating a Continent: Phylogenomics Reveal the Timing of Australian Frog Diversification
Authors: Brennan Ian G , Lemmon Alan R , Moriarty Lemmon Emily , Hoskin Conrad J , Donnellan Stephen C , Keogh J Scott
Published: August 2023

The Australian continent’s size and isolation make it an ideal place for studying the accumulation and evolution of biodiversity. Long separated from the ancient supercontinent Gondwana, most of Australia’s plants and animals are unique and endemic, including the continent’s frogs. Australian frogs comprise a remarkable ecological and morphological diversity categorized into a small number of distantly related radiations. We present a phylogenomic hypothesis based on an exon-capture dataset that spans the main clades of Australian myobatrachoid, pelodryadid hyloid, and microhylid frogs. Our time-calibrated phylogenomic-scale phylogeny identifies great disparity in the relative ages of these groups that vary from Gondwanan relics to recent immigrants from Asia and include arguably the continent’s oldest living vertebrate radiation. This age stratification provides insight into the colonization of, and diversification on, the Australian continent through deep time, during periods of dramatic climatic and community changes. Contemporary Australian frog diversity highlights the adaptive capacity of anurans, particularly in response to heat and aridity, and explains why they are one of the continent’s most visible faunas. [Anuran; adaptive radiation; Gondwana; phylogenetics].
Atopic dermatitis and ocular allergy: common mechanisms and uncommon questions
Authors: Ghosh Debajyoti , Mersha Tesfaye B.
Published: August 2023

Purpose of review

Atopic dermatitis (AD) and ocular allergy aka allergic eye disease (AED) are two common conditions that often coexist in patients. However, molecular connections between these two conditions are incompletely understood. While common etiologic components including Th2 immune signaling have been suggested for AD and AED, the mechanism how current Th2-targetd therapies (dupilumab, tralokinumab) for AD can augment conjunctivitis is not well understood.

Recent findings

Differentially regulated genes and pathways relevant for AD disease manifestation are known. In contrast, similar information is not yet available for AED, which could be largely addressed by emerging noninvasive ocular sampling techniques. Emerging evidence indicated a reduction in goblet cell number and mucin production in a subpopulation of AD patients with AD leading to adverse ocular outcomes, while other potential mechanisms could also be involved. Involvement of particular barrier function protein(s) in AED needs further investigation.

Modern cytokine-targeted therapies for AD showed elevated risk for developing conjunctivitis. Recently developed noninvasive sampling techniques should be leveraged to identify AD endotypes associated with AED and with dupilumab-associated ocular outcomes.