bluuebunny/crossref_metadata_2025 huggingface dataset:
The First Fossil Coelacanth from Thailand
Authors: Cavin Lionel , Tong Haiyan , Buffetaut Eric , Wongko Kamonlak , Suteethorn Varavudh , Deesri Uthumporn
Published: February 2023

Mawsoniidae is a family of coelacanths restricted to the Mesozoic. During the Cretaceous, mawsoniids were mainly represented by the Mawsonia/Axelrodichthy complex, long known to be from western Gondwana only (South America and Africa). This apparent biogeographical distribution then faded following the discovery of representatives in the Late Cretaceous of Laurasia (Europe and North America). We report here the presence, in the Lower Cretaceous site of Kham Phok, NE Thailand, of an angular bone referred to the Mawsonia/Axelrodichthys complex. A comparison with angulars referring to both genera found in various regions of the world between the Late Jurassic and the Late Cretaceous indicated that the distinctions between these genera, and even more so between their constituent species, are unclear. This discovery is further confirmation of the very slow morphological evolution within this lineage, which may explain why their evolutionary history appears to be disconnected, at least in part, from their geographical distribution over time.
First In Situ Observation of Sperm Release in Corynactis carnea (Anthozoa: Corallimorpharia) from Patagonia, Argentina
Authors: Bravo Gonzalo , Bigatti Gregorio , Penchaszadeh Pablo , Lauretta Daniel
Published: February 2023

Jewel sea anemones constitute a relatively small group of solitary cnidarians, a sister group of scleractinian corals. In the southwest Atlantic Ocean off Argentina, two species of jewel sea anemones have been found: Corynactis carnea and Corallimorphus rigidus. Corynactis carnea is a common and abundant species in shallow water of northern Atlantic Patagonia, but reproductive data on this species is scarce; the species is known to reproduce asexually. During a SCUBA diving survey in an Atlantic rocky reefs (20 m depth) in Patagonia, we observed for the first time specimens of C. carnea releasing sperm, eight days after the full moon and during the summer season while spawning has been previously observed in at least three species of Corynactis, but all were recorded as occurring in winter.
Online Channel Sales Premia in Times of COVID-19: First Evidence from Germany
Authors: Wagner Joachim
Published: February 2023

A presence on the web tends to be important for firms. Empirical studies show that firms with a better performance across various dimensions, and firms that are more internationally active, tend to have a website. Furthermore, a website helped firms to survive during the COVID-19 pandemic. An open question that is not discussed in the literature is how the use of online channels for sales is related to various dimensions of firm performance. This study contributes to the literature by using a unique recently released set of firm level data from Germany to investigate for the first time the links between online channels sales and firm characteristics. In a robustness check, the empirical investigation was replicated using strictly comparable firm level data from nine European countries, namely Austria, Belgium, Denmark, Finland, France, Ireland, Luxembourg, the Netherlands, and Sweden.
Relationship and Sexual Quality in the Wake of COVID-19: Effects of Individual Regulatory Focus and Shared Concerns over the Pandemic
Authors: Rodrigues David L. , Balzarini Rhonda N.
Published: February 2023

Research has shown mixed findings regarding the effect of the COVID-19 pandemic on relationship and sexual quality and activity. We argue that some of these findings might be understood considering people’s predisposition to maintain safety (i.e., prevention focus) or take risks (i.e., promotion focus), and sharing concerns with one’s partner about the pandemic. A longitudinal study (N = 153) tested if regulatory focus before the pandemic (November 2019) was associated with relationship quality, sexual quality, and joint sexual activity later on (June 2020) and whether these effects were moderated by shared concerns over the pandemic. Results showed that participants more focused on prevention experienced higher relationship quality later on, but also less sexual quality and less frequent joint sexual activity, when they shared fewer (vs. more) concerns with their partner. In contrast, participants more focused on promotion experienced higher relationship quality later on when they shared more (vs. less) concerns with their partner. These results indicate how individuals’ regulatory focus and shared concerns in times of crisis, such as the COVID-19 pandemic, can have downstream consequences on people’s relational and sexual dynamics. We offer insights for mental health professionals to improve psychosocial health and well-being when people are faced with critical events.
Prediction Model for Optimal Efficiency of the Green Corrosion Inhibitor Oleoylsarcosine: Optimization by Statistical Testing of the Relevant Influencing Factors
Authors: Kaskah Saad E. , Ehrenhaft Gitta , Gollnick Jörg , Fischer Christian B.
Published: February 2023

Optimization and statistical methods are used to minimize the number of experiments required to complete a study, especially in corrosion testing. Here, a statistical Box–Behnken design (BBD) was implemented to investigate the effects of four independent variables (inhibitor concentration [I], immersion time t, temperature ϑ, and NaCl content [NaCl]) based on the variation of three levels (lower, middle, and upper) on the corrosion protection efficiency of the green inhibitor oleoylsarcosine for low-carbon steel type CR4 in salt water. The effects of the selected variables were optimized using the response surface methodology (RSM) supported by the Minitab17 program. Depending on the BBD analytical tools, the largest effects were found for ϑ, followed by [I]. The effect of interactions between these variables was in the following order: [I] and ϑ > t and ϑ > [I] and [NaCl]. The second-order model used here for optimization showed that the upper level (+1) with 75 mmol/L for [I], 30 min for t, and 0.2 mol/L [NaCl] provided an optimal protective effect for each of these factors, while the lower level (−1) was 25 °C for ϑ. The theoretical efficiency predicted by the RSM model was 99.4%, while the efficiency during the experimental test procedure with the best-evaluated variables was 97.2%.
An Interactive Method for Detection of Process Activity Executions from IoT Data
Authors: Seiger Ronny , Franceschetti Marco , Weber Barbara
Published: February 2023

The increasing number of IoT devices equipped with sensors and actuators pervading every domain of everyday life allows for improved automated monitoring and analysis of processes executed in IoT-enabled environments. While sophisticated analysis methods exist to detect specific types of activities from low-level IoT data, a general approach for detecting activity executions that are part of more complex business processes does not exist. Moreover, dedicated information systems to orchestrate or monitor process executions are not available in typical IoT environments. As a consequence, the large corpus of existing process analysis and mining techniques to check and improve process executions cannot be applied. In this work, we develop an interactive method guiding the analysis of low-level IoT data with the goal of detecting higher-level process activity executions. The method is derived following the exploratory data analysis of an IoT data set from a smart factory. We propose analysis steps, sensor-actuator-activity patterns, and the novel concept of activity signatures that are applicable in many IoT domains. The method shows to be valuable for the early stages of IoT data analyses to build a ground truth based on domain knowledge and decisions of the process analyst, which can be used for automated activity detection in later stages.
Characterization of Histone Modifications in Late-Stage Rotator Cuff Tendinopathy
Authors: Orchard Kayleigh J. A. , Akbar Moeed , Crowe Lindsay A. N. , Cole John , Millar Neal L. , Raleigh Stuart M.
Published: February 2023

The development and progression of rotator cuff tendinopathy (RCT) is multifactorial and likely to manifest through a combination of extrinsic, intrinsic, and environmental factors, including genetics and epigenetics. However, the role of epigenetics in RCT, including the role of histone modification, is not well established. Using chromatin immunoprecipitation sequencing, differences in the trimethylation status of H3K4 and H3K27 histones in late-stage RCT compared to control were investigated in this study. For H3K4, 24 genomic loci were found to be significantly more trimethylated in RCT compared to control (p < 0.05), implicating genes such as DKK2, JAG2, and SMOC2 in RCT. For H3K27, 31 loci were shown to be more trimethylated (p < 0.05) in RCT compared to control, inferring a role for EPHA3, ROCK1, and DEFβ115. Furthermore, 14 loci were significantly less trimethylated (p < 0.05) in control compared to RCT, implicating EFNA5, GDF6, and GDF7. Finally, the TGFβ signaling, axon guidance, and regulation of focal adhesion assembly pathways were found to be enriched in RCT. These findings suggest that the development and progression of RCT is, at least in part, under epigenetic control, highlighting the influence of histone modifications in this disorder and paving the way to further understand the role of epigenome in RCT.
mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning
Authors: Banerjee Priyanka , Diniz Wellison J. S. , Hollingsworth Rachel , Rodning Soren P. , Dyce Paul W.
Published: February 2023

Reproductive failure is a major contributor to inefficiency within the cow-calf industry. Particularly problematic is the inability to diagnose heifer reproductive issues prior to pregnancy diagnosis following their first breeding season. Therefore, we hypothesized that gene expression from the peripheral white blood cells at weaning could predict the future reproductive potential of beef heifers. To investigate this, the gene expression was measured using RNA-Seq in Angus–Simmental crossbred heifers sampled at weaning and retrospectively classified as fertile (FH, n = 8) or subfertile (SFH, n = 7) after pregnancy diagnosis. We identified 92 differentially expressed genes between the groups. Network co-expression analysis identified 14 and 52 hub targets. ENSBTAG00000052659, OLR1, TFF2, and NAIP were exclusive hubs to the FH group, while 42 hubs were exclusive to the SFH group. The differential connectivity between the networks of each group revealed a gain in connectivity due to the rewiring of major regulators in the SFH group. The exclusive hub targets from FH were over-represented for the CXCR chemokine receptor pathway and inflammasome complex, while for the SFH, they were over-represented for immune response and cytokine production pathways. These multiple interactions revealed novel targets and pathways predicting reproductive potential at an early stage of heifer development.
Copy Number Variations in Children with Tourette Syndrome: Systematic Investigation in a Clinical Setting
Authors: Saia Federica , Prato Adriana , Saccuzzo Lucia , Madia Francesca , Barone Rita , Fichera Marco , Rizzo Renata
Published: February 2023

Tourette syndrome (TS) is a neurodevelopmental disturbance with heterogeneous and not completely known etiology. Clinical and molecular appraisal of affected patients is mandatory for outcome amelioration. The current study aimed to understand the molecular bases underpinning TS in a vast cohort of pediatric patients with TS. Molecular analyses included array-CGH analyses. The primary goal was to define the neurobehavioral phenotype of patients with or without pathogenic copy number variations (CNVs). Moreover, we compared the CNVs with CNVs described in the literature in neuropsychiatric disorders, including TS, to describe an effective clinical and molecular characterization of patients for prognostic purposes and for correctly taking charge. Moreover, this study showed that rare deletions and duplications focusing attention on significant genes for neurodevelopment had a statistically higher occurrence in children with tics and additional comorbidities. In our cohort, we determined an incidence of potentially causative CNVs of about 12%, in line with other literature studies. Clearly, further studies are needed to delineate the genetic background of patients with tic disorders in a superior way to elucidate the complex genetic architecture of these disorders, to describe the outcome, and to identify new possible therapeutic targets.
Oleate Impacts on Acetoclastic and Hydrogenotrophic Methanogenesis under Mesophilic and Thermophilic Conditions
Authors: Li Xiang , Yang Yang , Lu Chen-Shun , Kobayashi Takuro , Kong Zhe , Hu Yong
Published: February 2023

This study investigated oleate inhibition concentration on mesophilic and thermophilic sludge by utilizing acetate and H2/CO2 (80:20, v/v) as substrate, respectively. In addition, another batch experiment was carried out to explore the influence of oleate loads (mM-oleate/g-VS) on methane production. Generally, the mesophilic anaerobic system was more stable than the thermophilic system, which embodied higher microbial abundance, higher methane yield, and higher oleate tolerance. Furthermore, this study provides a possible methanogenic pathway impacted by oleate under mesophilic and thermophilic conditions according to functional microbial composition. Lastly, this paper provides noticeable and avoidable oleate concentrations and loads under different experimental conditions as a guide for future anaerobic bioreactors of lipidic waste biodegradation.
Spatial-Temporal Variations in of Soil Conservation Service and Its Influencing Factors under the Background of Ecological Engineering in the Taihang Mountain Area, China
Authors: Wang Feng , Liu Jintong , Fu Tonggang , Gao Hui , Qi Fei
Published: February 2023

Soil conservation (SC) plays an important role in maintaining regional land productivity and sustainable development. Ecological engineering (EE) is being implemented in different countries to effectively alleviate the damage to the ecological environment and effectively protect soil and food security. It is important to determine whether or not the SC capacity becomes stronger after the implementation of EE and whether or not EE has a notable impact on SC in different altitude zones. The exploration of the influencing mechanism and identification of the dominate influencing factors in different geographical regions needs to be improved. In this study, the soil conservation services (SCSs) from 1980 to 2020 in the Taihang Mountain area was assessed using the integrated valuation of ecosystem services and trade-offs (InVEST) model, and the spatial and temporal distributions and influencing factors were explored. The results showed the following: (1) the average SCSs exhibited an increasing trend from 1980 to 2020 on the whole, and the rate of increase reached 50.53% during the 41-year period. The rate of increase of the SCSs varied in the different EE implementation regions, and it was significantly higher than that of the entire study area. (2) The spatial distribution of the SCSs was highly heterogeneous, and the high SCS value areas were coincident with the high-altitude areas where forest and grassland occupied a large proportion. The low value areas were mainly located in the hilly zone or some of the basin regions where the proportion of construction land was relatively high. (3) The distribution pattern of the SCSs was the result of multiple factors. The EE intensity had the strongest explanatory power for the SCSs in the hilly zone, explaining 34.63%. The slope was the most critical factor affecting the SCSs in the mid-mountain and sub-alpine zones. The slope and normalized difference vegetation index (NDVI) had the greatest interactions with the other factors in the three altitude zones, especially in the high-altitude regions. The quantitative analysis of the SCSs and the influences of EE and natural factors on the SCSs revealed the heterogeneity in the mountainous areas. These results also provide a scientific basis for the reasonable implementation of EE and sustainable management of SCSs in the Taihang Mountain area.
Water Quality, Heavy Metals, and Antifungal Susceptibility to Fluconazole of Yeasts from Water Systems
Authors: Caicedo-Bejarano Luz Dary , Osorio-Vanegas Lizeth Stefania , Ramírez-Castrillón Mauricio , Castillo Jorge Enrique , Martínez-Garay Carlos Andrés , Chávez-Vivas Mónica
Published: February 2023

Aquatic environments could be reservoirs of pathogenic yeasts with acquired antifungal resistance. The susceptibility to antifungal agents of yeasts present in the wastewater and natural waters of the city of Cali was evaluated. Samples were taken from two types of water: drinking water (Meléndez River, drinking water treatment plant “Puerto Mallarino” in the Cauca River) and wastewater (South Channel of the Cauca River, “Cañaveralejo-PTAR” wastewater treatment plant). Physico-chemical parameters, heavy metal concentration, and yeast levels were determined using standard procedures. Yeasts were identified using API 20 C AUX (BioMérieux) and sequence analysis of the ITS1-5.8S-ITS2 and D1/D2 regions of the large subunit of the ribosome. Susceptibility assays against fluconazole and amphotericin B using the minimum inhibitory concentration (MIC) test were determined using the microdilution method. The influence of physico-chemical parameters and heavy metals was established using principal component analysis (PCA). Yeast counts were higher at WWTP “PTAR” and lower at Melendez River, as expected. A total of 14 genera and 21 yeast species was identified, and the genus Candida was present at all locations. Susceptibility tests showed a 32.7% resistance profile to fluconazole in the order DWTP “Puerto Mallarino = WWTP “PTAR” > South Channel “Navarro”. There were significant differences (p < 0.05) in the physico-chemical parameters/concentration of heavy metals and yeast levels between the aquatic systems under study. A positive association was observed between yeast levels and total dissolved solids, nitrate levels, and Cr at the “PTAR” WWTP; conductivity, Zn, and Cu in the South Channel; and the presence of Pb in the “Puerto Mallarino” DWTP. Rhodotorula mucilaginosa, Candida albicans, and Candida sp. 1 were influenced by Cr and Cd, and Diutina catelunata was influenced by Fe (p < 0.05). The water systems explored in this study showed different yeast levels and susceptibility profiles, and, therefore, possible genetic differences among populations of the same species, and different physico-chemical and heavy metals concentrations, which were probably modulating the antifungal-resistant yeasts. All these aquatic systems discharge their content into the Cauca River. We highlight the importance to further investigate if these resistant communities continue to other locations in the second largest river of Colombia and to determine the risk posed to humans and animals.
Demographic and Socioeconomic Factors Associated to Fruits and Vegetables Consumption in Elderly Europeans: A Systematic Review
Authors: Kouiti Malak , Ortega-Rico Carmen , Arrebola Juan Pedro , Gracia-Arnaiz Mabel , Larrea-Killinger Cristina
Published: February 2023

Several epidemiological studies stress the association between a diet based on high fruits and vegetables intake and a better health condition. However, elderly Europeans cannot manage the recommended fruits and vegetables consumption. This systematic review aims to explore the main factors related to fruits and vegetables consumption in elderly Europeans. We conducted literature searches on Medline, Scopus, and Web of Science from inception to May 2022. Published articles including data related to certain fruits and vegetables consumption among elderly Europeans were selected. The New Castle-Ottawa Scale and National Heart, Lung, and Blood Institute tools were used for methodological quality assessment by two authors independently. A total of 60 articles were retrieved, and data from twenty-one high-quality cross-sectional studies and five moderate-to-high-quality cohort studies, including a total of 109,516 participants, were synthesized. Associated factors mostly analyzed were those relating to demographic and socioeconomic status, such as sex, age, marital status, educational level, and income. However, the findings show a high discrepancy. Some evidence suggests a possible positive association, while other evidence shows an inverse or no association at all. The relationship between demographic and socioeconomic factors with fruits and vegetables consumption is not at all clear. More epidemiological studies with an appropriate design and corresponding statistical methods are required.
Reforms in China’s Vaccine Administration—From the Perspective of New Governance Approach
Authors: Tang Lin , Zhang Lingling
Published: February 2023

Recent vaccine scandals have overshadowed China’s accomplishments in public health, triggering discussions on the causes of vaccine incidents. This study aims to review the development of China’s vaccine administration, find out the causes of recurring vaccine incidents in the past decades, and propose a new governance approach to vaccine administration in the context of a public resource trading system. We collect and analyze relevant legal frameworks and data from legislative materials, government documents, press releases, and reports from the World Health Organization. In essence, it is the combination of the lagging legal system and the absence of information technology infrastructure in the process of vaccine administration reform that has led to the recurrence of vaccine incidents. Though the vaccine incidents occurred concentratedly in phases of production and lot release, and circulation, it is necessary to examine the whole life cycle of vaccine administration. The enactment of the Vaccine Administration Law outlines a supervision framework, which utilizes the Whole Process Electronic Traceability System and Whole Life-cycle Quality Management System to achieve the interconnection of all aspects of vaccine administration. The reform of China’s vaccine administration is essentially a balance between efficiency and safety, which also represents the interaction between marketization and administrative supervision.
A Person-Environment Fit Model to Explain Information and Communication Technologies-Enabled After-Hours Work-Related Interruptions in China
Authors: Zhang Shanshan , Huang Fengchun , Zhang Yuting , Li Qiwen
Published: February 2023

Given the ubiquitous nature of mobile devices and information and communication technologies (ICT), after-hours work-related interruptions (AHWI) occur anywhere and anytime in China. In the current study, an alternative person–environment (P-E) fit model of ICT-enabled AHWI, hereafter referred to as IAWI, that treats polychronic variables as moderated solutions are presented. A cross-sectional survey among 277 Chinese employees (average age: 32.04 years) was conducted in September 2022 and tested by PLS-structural equation modeling to validate our hypotheses. The results indicated that IAWI had a positive influence on employees’ innovative job performance and in-role job performance (β = 0.139, p < 0.05; β = 0.200, p < 0.01; β = 0.298, p < 0.001). Moreover, among employees with higher levels of polychronicity, the heightened effects of IAWI on innovative job performance were increased (β = 0.112, p < 0.05). This study offers implications for employees: under IAWI situations, they could search for a person–environment (P-E) that is fit to buffer the negative aspects of IAWI, consequently increasing their innovative job performance and in-role job performance. Future research could extend beyond this framework to explore employees’ IAWI and job performance balance.
The Interrelationship of Loneliness, Smartphone Addiction, Sleep Quality, and Students’ Attention in English as a Foreign Language Class
Authors: Kao Po-Chi
Published: February 2023

In this study, a research model comprising four variables (loneliness, smartphone addiction, sleep quality, and students’ attention in English as a foreign language class) was proposed and statistically examined. Previous literature has appeared to neglect these variables, which are considered to be essential to understanding students’ attention in EFL (English as a foreign language) class among college students. A total of 587 undergraduate students were recruited from a university in Taiwan to participate in the present study. The technique of structural equation modeling was adopted to test the hypotheses in the conceptual model. The findings of this study are: (1) smartphone addiction has a significant negative impact on students’ attention in EFL class; (2) smartphone addiction has a significant negative impact on sleep quality; (3) sleep quality has a significant positive impact on students’ attention in EFL class; (4) sleep quality partially mediates the relationship between smartphone addiction and students’ attention in EFL class; (5) loneliness has a significant positive effect on smartphone addiction. The results can enrich the present literature in the psychology of attention and mobile technology by providing an insight into the dynamics of these four variables.
Evaluation of Attitudes toward Corporal Expression in Primary School Students: Validation and Psychometric Properties of a Questionnaire
Authors: Rojo-Ramos Jorge , Polo-Campos Irene , Hernández-Mosqueira Claudio , Gomez-Paniagua Santiago
Published: February 2023

The content of corporal expression, usually forgotten by some teachers, has been shown to have favorable effects on the physical, social, and psychological health of students at all levels of education. Likewise, students’ attitudes should be positively influenced in the school environment in order to improve the teaching–learning process of the different contents taught. The purpose of this study was to provide the factor structure and validity of a questionnaire used to gauge pupils’ attitudes toward corporal expression. The sample consisted of 709 students in the last cycle of primary school from schools in the region of Extremadura (Spain). Reliability testing as well as confirmatory and exploratory factor analyses were conducted. The findings revealed a factor structure with three dimensions composed of 30 items with high reliability (Cronbach’s alpha = 0.85–0.90) and good and exceptional goodness-of-fit values. As a result, the questionnaire can be seen as a quick and simple instrument to use in analyzing students’ attitudes toward corporal expression and enabling stakeholders to take action to support it.
Online Health Information-Seeking Behaviours and eHealth Literacy among First-Generation Chinese Immigrants
Authors: Zhang Ling , Chung Sherrie , Shi Wendan , Candelaria Dion , Gallagher Robyn
Published: February 2023

Due to linguistic and cultural barriers, immigrants often have limited access to health information. Online health information is popular and accessible, but quality is questionable and its benefits dependent on an individual’s eHealth literacy. This study examined online health information-seeking behaviours, eHealth literacy and its predictors among first-generation Chinese immigrants. A sample of 356 Chinese immigrants living in Australia completed an anonymous paper-based survey, including sociodemographic, clinical data, English proficiency, health literacy, online health information-seeking behaviours, and eHealth literacy. Linear regression models analyzed predictive factors of eHealth literacy. Participants were aged mean 59.3 years, female (68.3%), 53.1% completed university, and their English proficiency was rated fair/poor by 75.1%. Participants perceived online health information as useful (61.6%) and important (56.2%) to their health. Health information accessed was often related to lifestyle (61.2%), health resources (44.9%), diseases (36.0%), and medications (30.9%). Inadequate health literacy and eHealth literacy occurred in 48.3% and 44.9%, respectively. Age, number of technological devices used, education, and health status were independently associated with eHealth literacy. While most Chinese immigrants used online health information, many had inadequate eHealth literacy. Healthcare authorities and providers should support older immigrants, those with lower education and poorer health, and those less engaged with technology in online health information use by providing culturally and linguistically appropriate information, directing immigrants to credible websites, and involving them in health material development processes.
More Competition in Mind, Better Sleep at Night? The Mediating Role of Anxiety between Competitive Attitude and Sleep Quality
Authors: Ou Kaitong , Ma Ning
Published: February 2023

Previous studies have suggested that individuals with hypercompetitive attitude and interpersonal insecurity would have a high level of anxiety, and anxiety has been found to strongly impact on sleep quality. However, the associations between competitive attitudes and sleep quality have not been studied until now. The present study aimed to examine whether anxiety mediates the relationship between competitive attitudes and interpersonal relationships with sleep quality. This was a cross-sectional study with a total of 713 college students (age = 20.18 ± 2.16 years old; 78.8% female) recruited online to measure hypercompetitive attitude, personal development competitive attitude, interpersonal security, state anxiety and sleep quality. Path analysis models were conducted in this study. The path analysis models showed that both hypercompetitive attitude and interpersonal security had direct and indirect significant effects on poor sleep quality due to the mediating effect of state anxiety (β = 0.023, 95% bootstrapped CI: 0.005 to 0.047; β = −0.051, 95% bootstrapped CI: −0.099 to −0.010, respectively). However, personal development competitive attitude had only an indirect significant effect, but it had a negative role on poor sleep quality via state anxiety (β = −0.021, 95% bootstrapped CI: −0.042 to −0.008). The current study provided evidence that college students’ competitive attitudes would impact sleep quality and highlighted the mediating role of state anxiety. The current findings suggested that individuals shifting their hypercompetitive thinking to concentrate on ability development would benefit their mental health.
Effects of Live Music on the Perception of Noise in the SICU/PICU: A Patient, Caregiver, and Medical Staff Environmental Study
Authors: Rossetti Andrew , Loewy Joanne , Chang-Lit Wen , van Dokkum Nienke H. , Baumann Erik , Bouissou Gabrielle , Mondanaro John , O’Connor Todd , Asch-Ortiz Gabriela , Mitaka Hayato
Published: February 2023

Intensive Care Units (ICUs) require a multidisciplinary team that consists of, but is not limited to, intensivists (clinicians who specialize in critical illness care), pharmacists and nurses, respiratory care therapists, and other medical consultants from a broad range of specialties. The complex and demanding critical care environment provides few opportunities for patients and personal and professional caregivers to evaluate how sound effects them. A growing body of literature attests to noise’s adverse influence on patients’ sleep, and high sound levels are a source of staff stress, as noise is an ubiquitous and noxious stimuli. Vulnerable patients have a low threshold tolerance to audio-induced stress. Despite these indications, peak sound levels often register as high, as can ventilators, and the documented noise levels in hospitals continue to rise. This baseline study, carried out in two hospitals’ Surgical and Pediatric Intensive Care Units, measured the effects of live music on the perception of noise through surveying patients, personal caregivers and staff in randomized conditions of no music, and music as provided by music therapists through our hospital system’s environmental music therapy program.
Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification
Authors: Brogna Carlo , Costanzo Vincenzo , Brogna Barbara , Bisaccia Domenico Rocco , Brogna Giancarlo , Giuliano Marino , Montano Luigi , Viduto Valentina , Cristoni Simone , Fabrowski Mark , Piscopo Marina
Published: February 2023

SARS-CoV-2, one of the human RNA viruses, is widely studied around the world. Significant efforts have been made to understand its molecular mechanisms of action and how it interacts with epithelial cells and the human microbiome since it has also been observed in gut microbiome bacteria. Many studies emphasize the importance of surface immunity and also that the mucosal system is critical in the interaction of the pathogen with the cells of the oral, nasal, pharyngeal, and intestinal epithelium. Recent studies have shown how bacteria in the human gut microbiome produce toxins capable of altering the classical mechanisms of interaction of viruses with surface cells. This paper presents a simple approach to highlight the initial behavior of a novel pathogen, SARS-CoV-2, on the human microbiome. The immunofluorescence microscopy technique can be combined with spectral counting performed at mass spectrometry of viral peptides in bacterial cultures, along with identification of the presence of D-amino acids within viral peptides in bacterial cultures and in patients’ blood. This approach makes it possible to establish the possible expression or increase of viral RNA viruses in general and SARS-CoV-2, as discussed in this study, and to determine whether or not the microbiome is involved in the pathogenetic mechanisms of the viruses. This novel combined approach can provide information more rapidly, avoiding the biases of virological diagnosis and identifying whether a virus can interact with, bind to, and infect bacteria and epithelial cells. Understanding whether some viruses have bacteriophagic behavior allows vaccine therapies to be focused either toward certain toxins produced by bacteria in the microbiome or toward finding inert or symbiotic viral mutations with the human microbiome. This new knowledge opens a scenario on a possible future vaccine: the probiotics vaccine, engineered with the right resistance to viruses that attach to both the epithelium human surface and gut microbiome bacteria.
Antibacterial Activity of Juglone Revealed in a Wound Model of Staphylococcus aureus Infection
Authors: Wan Yangli , Wang Xiaowen , Yang Liu , Li Qianhong , Zheng Xuting , Bai Tianyi , Wang Xin
Published: February 2023

A serious problem currently facing the field of wound healing is bacterial infection, especially Staphylococcus aureus (S. aureus) infection. Although the application of antibiotics has achieved good effects, their irregular use has resulted in the emergence of drug-resistant strains. It is thus the purpose of this study to analyze whether the naturally extracted phenolic compound, juglone, can inhibit S. aureus in wound infection. The results show that the minimum inhibitory concentration (MIC) of juglone against S. aureus was 1000 μg/mL. Juglone inhibited the growth of S. aureus by inhibiting membrane integrity and causing protein leakage. At sub-inhibitory concentrations, juglone inhibited biofilm formation, the expression of α-hemolysin, the hemolytic activity, and the production of proteases and lipases of S. aureus. When applied to infected wounds in Kunming mice, juglone (50 μL juglone with a concentration of 1000 μg/mL) significantly inhibited the number of S. aureus and had a significant inhibitory effect on the expression of inflammatory mediators (TNF-α, IL-6 and IL-1β). Moreover, the juglone-treated group promoted wound healing. At the same time, in animal toxicity experiments, juglone had no obvious toxic effects on the main tissues and organs of mice, indicating that juglone has good biocompatibility and has the potential to be used in the treatment of wounds infected with S. aureus.
S-Locus Genotyping in Japanese Plum by High Throughput Sequencing Using a Synthetic S-Loci Reference Sequence
Authors: Hedhly Afif , Guerra María Engracia , Grimplet Jerome , Rodrigo Javier
Published: February 2023

Self-incompatibility in Prunus species is governed by a single locus consisting of two highly multi-allelic and tightly linked genes, one coding for an F-box protein—i.e., SFB in Prunus- controlling the pollen specificity and one coding for an S-RNase gene controlling the pistil specificity. Genotyping the allelic combination in a fruit tree species is an essential procedure both for cross-based breeding and for establishing pollination requirements. Gel-based PCR techniques using primer pairs designed from conserved regions and spanning polymorphic intronic regions are traditionally used for this task. However, with the great advance of massive sequencing techniques and the lowering of sequencing costs, new genotyping-by-sequencing procedures are emerging. The alignment of resequenced individuals to reference genomes, commonly used for polymorphism detection, yields little or no coverage in the S-locus region due to high polymorphism between different alleles within the same species, and cannot be used for this purpose. Using the available sequences of Japanese plum S-loci concatenated in a rosary-like structure as synthetic reference sequence, we describe a procedure to accurately genotype resequenced individuals that allowed the analysis of the S-genotype in 88 Japanese plum cultivars, 74 of them are reported for the first time. In addition to unraveling two new S-alleles from published reference genomes, we identified at least two S-alleles in 74 cultivars. According to their S-allele composition, they were assigned to 22 incompatibility groups, including nine new incompatibility groups reported here for the first time (XXVII-XXXV).
Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
Authors: Artyukhin Alexander , Sharifyanova Yuliya , Krivosheev Mikhail M. , Mikhaylova Elena V.
Published: February 2023

The larches of Kuzhanovo (Larix sibirica Ledeb.) are protected trees with a round crown growing in the Southern Urals. In 2020 vandals sawed the sapwood of these trees, which exposed the problem of insufficient conservation measures. Their origin and genetic characteristics have been of particular interest to breeders and scientists. The larches of Kuzhanovo were screened for polymorphisms using SSR and ISSR analyses and the sequencing of genetic markers and genes GIGANTEA and mTERF, associated with wider crown shape. A unique mutation was discovered in the atpF–atpH intergenic spacer of all protected trees, but it was absent in some of their descendants and larches with similar crown shape. Mutations were discovered in the rpoC1 and mTERF genes of all samples. Flow cytometry did not reveal any changes in genome size. Our results suggest that the unique phenotype arose from point mutations in L. sibirica, but they are yet to be found in the nuclear genome. The concurrent mutations in the rpoC1 and mTERF genes may indicate that the round crown shape originates from the Southern Urals. The atpF–atpH and rpoC1 genetic markers are not common in studies of Larix sp., but their wider use could help to establish the origin of these endangered plants. The discovery of the unique atpF–atpH mutation also allows for stronger conservation and crime detection efforts.
Emerging Role of Cancer-Associated Fibroblasts in Progression and Treatment of Hepatocellular Carcinoma
Authors: Akkız Hikmet
Published: February 2023

Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide and the fourth leading cause of cancer-related death globally. Tumor cells recruit and remodel various types of stromal and inflammatory cells to form a tumor microenvironment (TME), which encompasses cellular and molecular entities, including cancer-associated fibroblasts (CAFs), tumor-associated macrophages (TAMs), tumor-associated neutrophils (TANs), immune cells, myeloid-derived suppressor cells (MDSCs), immune checkpoint molecules and cytokines that promote cancer cell growth, as well as their drug resistance. HCC usually arises in the context of cirrhosis, which is always associated with an enrichment of activated fibroblasts that are owed to chronic inflammation. CAFs are a major component of the TME, providing physical support in it and secreting various proteins, such as extracellular matrices (ECMs), hepatocyte growth factor (HGF), insulin-like growth factor 1/2 (ILGF1/2) and cytokines that can modulate tumor growth and survival. As such, CAF-derived signaling may increase the pool of resistant cells, thus reducing the duration of clinical responses and increasing the degree of heterogeneity within tumors. Although CAFs are often implicated to be associated with tumor growth, metastasis and drug resistance, several studies have reported that CAFs have significant phenotypic and functional heterogeneity, and some CAFs display antitumor and drug-sensitizing properties. Multiple studies have highlighted the relevance of crosstalk between HCC cells, CAFs and other stromal cells in influence of HCC progression. Although basic and clinical studies partially revealed the emerging roles of CAFs in immunotherapy resistance and immune evasion, a better understanding of the unique functions of CAFs in HCC progression will contribute to development of more effective molecular-targeted drugs. In this review article, molecular mechanisms involved in crosstalk between CAFs, HCC cells and other stromal cells, as well as the effects of CAFs on HCC-cell growth, metastasis, drug resistance and clinical outcomes, are comprehensively discussed.
Biophysical Parameters Can Induce Epithelial-to-Mesenchymal Phenotypic and Genotypic Changes in HT-29 Cells: A Preliminary Study
Authors: Pape Judith , Micalet Auxtine , Alsheikh Wissal , Ezbakh Nadia , Virjee Rania-Iman , Al Hosni Rawiya , Moeendarbary Emad , Cheema Umber
Published: February 2023

Epithelial to mesenchymal transition (EMT) in cancer is the process described where cancer epithelial cells acquire mesenchymal properties which can lead to enhanced invasiveness. Three-dimensional cancer models often lack the relevant and biomimetic microenvironment parameters appropriate to the native tumour microenvironment thought to drive EMT. In this study, HT-29 epithelial colorectal cells were cultivated in different oxygen and collagen concentrations to investigate how these biophysical parameters influenced invasion patterns and EMT. Colorectal HT-29 cells were grown in physiological hypoxia (5% O2) and normoxia (21% O2) in 2D, 3D soft (60 Pa), and 3D stiff (4 kPa) collagen matrices. Physiological hypoxia was sufficient to trigger expression of markers of EMT in the HT-29 cells in 2D by day 7. This is in contrast to a control breast cancer cell line, MDA-MB-231, which expresses a mesenchymal phenotype regardless of the oxygen concentration. In 3D, HT-29 cells invaded more extensively in a stiff matrix environment with corresponding increases in the invasive genes MMP2 and RAE1. This demonstrates that the physiological environment can directly impact HT-29 cells in terms of EMT marker expression and invasion, compared to an established cell line, MDA-MB-231, which has already undergone EMT. This study highlights the importance of the biophysical microenvironment to cancer epithelial cells and how these factors can direct cell behaviour. In particular, that stiffness of the 3D matrix drives greater invasion in HT-29 cells regardless of hypoxia. It is also pertinent that some cell lines (already having undergone EMT) are not as sensitive to the biophysical features of their microenvironment.
Transcriptome and DNA Methylome Analysis of Two Contrasting Rice Genotypes under Salt Stress during Germination
Authors: Li Yongqiang , Guo Dianjing
Published: February 2023

With climate change and labor shortages, direct-seeding rice cultivation is becoming popular worldwide, especially in Asia. Salinity stress negatively affects rice seed germination in the direct-seeding process, and the cultivation of suitable direct-seeding rice varieties under salinity stress is necessary. However, little is known about the underlying mechanism of salt responses during seed germination under salt stress. To investigate the salt tolerance mechanism at the seed germination stage, two contrasting rice genotypes differing in salt tolerance, namely, FL478 (salt-tolerant) and IR29 (salt-sensitive), were used in this study. We observed, that compared to IR29, FL478 appeared to be more tolerant to salt stress with a higher germination rate. GD1 (germination defective 1), which was involved in seed germination by regulating alpha-amylase, was upregulated significantly in the salt-sensitive IR29 strain under salt stress during germination. Transcriptomic data showed that salt-responsive genes tended to be up/downregulated in IR29 but not in FL478. Furthermore, we investigated the epigenetic changes in FL478 and IR29 during germination under saline treatment using whole genome bisulfite DNA sequencing (BS-seq) technology. BS-seq data showed that the global CHH methylation level increased dramatically under salinity stress in both strains, and the hyper CHH differentially methylated regions (DMRs) were predominantly located within the transposable elements regions. Compared with FL478, differentially expressed genes with DMRs in IR29 were mainly related to gene ontology terms such as response to water deprivation, response to salt stress, seed germination, and response to hydrogen peroxide pathways. These results may provide valuable insights into the genetic and epigenetic basis of salt tolerance at the seed germination stage, which is important for direct-seeding rice breeding.
Imaging and Characterization of Oxidative Protein Modifications in Skin
Authors: Prasad Ankush , Duchová Hana , Manoharan Renuka Ramalingam , Rathi Deepak , Pospíšil Pavel
Published: February 2023

Skin plays an important role in protection, metabolism, thermoregulation, sensation, and excretion whilst being consistently exposed to environmental aggression, including biotic and abiotic stresses. During the generation of oxidative stress in the skin, the epidermal and dermal cells are generally regarded as the most affected regions. The participation of reactive oxygen species (ROS) as a result of environmental fluctuations has been experimentally proven by several researchers and is well known to contribute to ultra-weak photon emission via the oxidation of biomolecules (lipids, proteins, and nucleic acids). More recently, ultra-weak photon emission detection techniques have been introduced to investigate the conditions of oxidative stress in various living systems in in vivo, ex vivo and in vitro studies. Research into two-dimensional photon imaging is drawing growing attention because of its application as a non-invasive tool. We monitored spontaneous and stress-induced ultra-weak photon emission under the exogenous application of a Fenton reagent. The results showed a marked difference in the ultra-weak photon emission. Overall, these results suggest that triplet carbonyl (3C=O∗) and singlet oxygen (1O2) are the final emitters. Furthermore, the formation of oxidatively modified protein adducts and protein carbonyl formation upon treatment with hydrogen peroxide (H2O2) were observed using an immunoblotting assay. The results from this study broaden our understanding of the mechanism of the generation of ROS in skin layers and the formation/contribution of various excited species can be used as tools to determine the physiological state of the organism.