bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental extractable work-based multipartite separability criteria
Authors: Ciampini Mario A. , Mancino Luca , Orieux Adeline , Vigliar Caterina , Mataloni Paolo , Paternostro Mauro , Barbieri Marco
Published: March 2017

A thermodynamic theory of quantum entanglement as well as the establishment of rigorous formal connections between the laws of thermodynamics and the phenomenology of entanglement are currently open areas of investigation. In this quest, a core problem is embodied by the understanding of the role that entanglement plays in processes of work extraction. Here, by considering information thermodynamics-based protocols, we answer the question “Is it possible to infer, quantitatively, quantum correlations by considering work-extraction schemes?”. Our experimental settings consist of suitably designed multi-photon optical interferometers able to address the case of both bipartite and multipartite entangled states. We compare the performance of such criteria to that of witnesses of entanglement based on the violation of Bell-like tests, showing their inherently different nature. Our work contributes strongly to the ongoing efforts in establishing photonic systems as a platform for experiments in information thermodynamics.
Identification of multiple functional receptors for tyramine on an insect secretory epithelium
Authors: Zhang Haiying , Blumenthal Edward M.
Published: March 2017

The biogenic amine tyramine (TA) regulates many aspects of invertebrate physiology and development. Although three TA receptor subtypes have been identified (TAR1-3), specific receptors have not been linked to physiological responses in native tissue. In the Malpighian (renal) tubule of Drosophila melanogaster, TA activates a transepithelial chloride conductance, resulting in diuresis and depolarization of the transepithelial potential. In the current work, mutation or RNAi-mediated knockdown in the stellate cells of the tubule of TAR2 (tyrR, CG7431) resulted in a dramatic reduction, but not elimination, of the TA-mediated depolarization. Mutation or knockdown of TAR3 (tyrRII, CG16766) had no effect. However, deletion of both genes, or knockdown of TAR3 on a TAR2 mutant background, eliminated the TA responses. Thus while TAR2 is responsible for the majority of the TA sensitivity of the tubule, TAR3 also contributes to the response. Knockdown or mutation of TAR2 also eliminated the response of tubules to the related amine octopamine (OA), indicating that OA can activate TAR2. This finding contrasts to reports that heterologously expressed TAR2 is highly selective for TA over OA. This is the first report of TA receptor function in a native tissue and indicates unexpected complexity in the physiology of the Malpighian tubule.
Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
Authors: O’Halloran Damien M. , Altshuler-Keylin Svetlana , Zhang Xiao-Dong , He Chao , Morales-Phan Christopher , Yu Yawei , Kaye Julia A. , Brueggemann Chantal , Chen Tsung-Yu , L’Etoile Noelle D.
Published: March 2017

InCaenorhabditis elegans, the AWC neurons are thought to deploy a cGMP signaling cascade in the detection of and response to AWC sensed odors. Prolonged exposure to an AWC sensed odor in the absence of food leads to reversible decreases in the animal’s attraction to that odor. This adaptation exhibits two stages referred to as short-term and long-term adaptation. Previously, the protein kinase G (PKG), EGL-4/PKG-1, was shown necessary for both stages of adaptation and phosphorylation of its target, the beta-type cyclic nucleotide gated (CNG) channel subunit, TAX-2, was implicated in the short term stage. Here we uncover a novel role for the CNG channel subunit, CNG-3, in short term adaptation. We demonstrate that CNG-3 is required in the AWC for adaptation to short (thirty minute) exposures of odor, and contains a candidate PKG phosphorylation site required to tune odor sensitivity. We also providein vivodata suggesting that CNG-3 forms a complex with both TAX-2 and TAX-4 CNG channel subunits in AWC. Finally, we examine the physiology of different CNG channel subunit combinations.
Correlation of Ultrasound Shear Wave Elastography with Pathological Analysis in a Xenografic Tumour Model
Authors: Elyas Eli , Papaevangelou Efthymia , Alles Erwin J. , Erler Janine T. , Cox Thomas R. , Robinson Simon P. , Bamber Jeffrey C.
Published: March 2017

The objective of this study was to evaluate the potential value of ultrasound (US) shear wave elastography (SWE) in assessing the relative change in elastic modulus in colorectal adenocarcinoma xenograft models in vivo and investigate any correlation with histological analysis. We sought to test whether non-invasive evaluation of tissue stiffness is indicative of pathological tumour changes and can be used to monitor therapeutic efficacy. US-SWE was performed in tumour xenografts in 15 NCr nude immunodeficient mice, which were treated with either the cytotoxic drug, Irinotecan, or saline as control. Ten tumours were imaged 48 hours post-treatment and five tumours were imaged for up to five times after treatment. All tumours were harvested for histological analysis and comparison with elasticity measurements. Elastic (Young's) modulus prior to treatment was correlated with tumour volume (r = 0.37, p = 0.008). Irinotecan administration caused significant delay in the tumour growth (p = 0.02) when compared to control, but no significant difference in elastic modulus was detected. Histological analysis revealed a significant correlation between tumour necrosis and elastic modulus (r = −0.73, p = 0.026). SWE measurement provided complimentary information to other imaging modalities and could indicate potential changes in the mechanical properties of tumours, which in turn could be related to the stages of tumour development.
Network Anatomy Controlling Abrupt-like Percolation Transition
Authors: Kawamoto Hirokazu , Takayasu Hideki , Takayasu Misako
Published: March 2017

We virtually dissect complex networks in order to understand their internal structure, just as doctors do with the bodies of animals. Our novel method classifies network links into four categories: bone, fat, cartilage, and muscle, based on network connectivity. We derive an efficient percolation strategy from this new viewpoint of network anatomy, which enables abrupt-like percolation transition through removal of a small amount of cartilage links, which play a crucial role in network connectivity. Furthermore, we find nontrivial scaling laws in the relationships between four types of links in each cluster and evaluate power exponents, which characterize network structures as seen in the real large-scale network of trading business firms and in the Erdős-Rényi network. Finally, we observe changes in the transition point for random bond percolation process, demonstrating that the addition of muscle links enhances network robustness, while fat links are irrelevant. These findings aid in controlling the percolation transition for an arbitrary network.
Unsuppressible Repetition Suppression and exemplar-specific Expectation Suppression in the Fusiform Face Area
Authors: Pajani Auréliane , Kouider Sid , Roux Paul , de Gardelle Vincent
Published: March 2017

Recent work casts Repetition Suppression (RS), i.e. the reduced neural response to repeated stimuli, as the consequence of reduced surprise for repeated inputs. This research, along with other studies documenting Expectation Suppression, i.e. reduced responses to expected stimuli, emphasizes the role of expectations and predictive codes in perception. Here, we use fMRI to further characterize the nature of predictive signals in the human brain. Prior to scanning, participants were implicitly exposed to associations within face pairs. Critically, we found that this resulted in exemplar-specific Expectation Suppression in the fusiform face-sensitive area (FFA): individual faces that could be predicted from the associations elicited reduced FFA responses, as compared to unpredictable faces. Thus, predictive signals in the FFA are specific to face exemplars, and not only generic to the category of face stimuli. In addition, we show that under such circumstances, the occurrence of surprising repetitions did not trigger enhanced brain responses, as had been recently hypothesized, but still suppressed responses, suggesting that repetition suppression might be partly ‘unsuppressible’. Repetition effects cannot be fully modulated by expectations, which supports the recent view that expectation and repetition effects rest on partially independent mechanisms. Altogether, our study sheds light on the nature of expectation signals along the perceptual system.
Surface Current in “Hotspot” Serves as a New and Effective Precursor for El Niño Prediction
Authors: Wang Jianing , Lu Youyu , Wang Fan , Zhang Rong-Hua
Published: March 2017

The El Niño and Southern Oscillation (ENSO) is the most prominent sources of inter-annual climate variability. Related to the seasonal phase-locking, ENSO’s prediction across the low-persistence barrier in the boreal spring remains a challenge. Here we identify regions where surface current variability influences the short-lead time predictions of the July Niño 3.4 index by applying a regression analysis. A highly influential region, related to the distribution of wind-stress curl and sea surface temperature, is located near the dateline and the southern edge of the South Equatorial Current. During El Niño years, a westward current anomaly in the identified high-influence region favours the accumulation of warm water in the western Pacific. The opposite occurs during La Niña years. This process is seen to serve as the “goal shot” for ENSO development, which provides an effective precursor for the prediction of the July Niño 3.4 index with a lead time of 2–4 months. The prediction skill based on surface current precursor beats that based on the warm water volume and persistence in the subsequent months after July. In particular, prediction based on surface current precursor shows skill in all years, while predictions based on other precursors show reduced skill after 2002.
Anisotropy in the thermal hysteresis of resistivity and charge density wave nature of single crystal SrFeO3-δ: X-ray absorption and photoemission studies
Authors: Hsieh S. H. , Solanki R. S. , Wang Y. F. , Shao Y. C. , Lee S. H. , Yao C. H. , Du C. H. , Wang H. T. , Chiou J. W. , Chin Y. Y. , Tsai H. M. , Chen J.-L. , Pao C. W. , Cheng C.-M. , Chen W.-C. , Lin H. J. , Lee J. F. , Chou F. C. , Pong W. F.
Published: March 2017

The local electronic and atomic structures of the high-quality single crystal of SrFeO3-δ (δ~0.19) were studied using temperature-dependent x-ray absorption and valence-band photoemission spectroscopy (VB-PES) to investigate the origin of anisotropic resistivity in the ab-plane and along the c-axis close to the region of thermal hysteresis (near temperature for susceptibility maximum, Tm~78 K). All experiments herein were conducted during warming and cooling processes. The Fe L3,2-edge X-ray linear dichroism results show that during cooling from room temperature to below the transition temperature, the unoccupied Fe 3d eg states remain in persistently out-of-plane 3d3z2-r2 orbitals. In contrast, in the warming process below the transition temperature, they change from 3d3z2-r2 to in-plane 3dx2-y2 orbitals. The nearest-neighbor (NN) Fe-O bond lengths also exhibit anisotropic behavior in the ab-plane and along the c-axis below Tm. The anisotropic NN Fe-O bond lengths and Debye-Waller factors stabilize the in-plane Fe 3dx2-y2 and out-of-plane 3d3z2-r2 orbitals during warming and cooling, respectively. Additionally, a VB-PES study further confirms that a relative band gap opens at low temperature in both the ab-plane and along the c-axis, providing the clear evidence of the charge-density-wave nature of SrFeO3-δ (δ~0.19) single crystal.
High Performance Solution Processed Organic Field Effect Transistors with Novel Diketopyrrolopyrrole-Containing Small Molecules
Authors: Lim Bogyu , Sun Huabin , Lee Jaechol , Noh Yong-Young
Published: March 2017

The donor-acceptor (D-A)-type diketopyrrolopyrrole (DPP)-based small molecules (LGC-D117 and LGC-D118) were synthesized and used as the active layer of solution-processable organic field-effect transistors (OFETs). Both LGC-D117 and LGC-D118 contain silaindacenodithiophene as electron-donor units with DPP as an electron-accepting linker, and octylrhodanine as the electron-accepting end group. The molecules were functionalized with different side chains to study their effects on OFET characteristics. LGC-D117 has a simple branched alkyl side chain, whereas LGC-D118 features a bulky siloxane-terminated hybrid alkyl chain. The siloxane side chains of LGC-D118 account for its better crystallinity, leading to significantly high field-effect mobility (max 3.04 cm2 V−1 s−1). In particular, LGC-D118 is well soluble and sustains the high mobility in the environmentally friendly 2-methyltetrahydrofuran solvent with low temperature annealing at 100 °C due to the bulky siloxane-terminated alkyl side chain.
Arsenic Methylation and its Relationship to Abundance and Diversity of arsM Genes in Composting Manure
Authors: Zhai Weiwei , Wong Mabel T. , Luo Fei , Hashmi Muhammad Z. , Liu Xingmei , Edwards Elizabeth A. , Tang Xianjin , Xu Jianming
Published: March 2017

Although methylation is regarded as one of the main detoxification pathways for arsenic (As), current knowledge about this process during manure composting remains limited. In this study, two pilot-scale compost piles were established to treat manure contaminated with As. An overall accumulation of methylated As occurred during 60 day-composting time. The concentration of monomethylarsonic acid (MMA) increased from 6 to 190 μg kg−1 within 15 days and decreased to 35 μg kg−1 at the end of the maturing phase; while the concentration of dimethylarsinic acid (DMA) continuously increased from 33 to 595 μg kg−1 over the composting time. The arsM gene copies increased gradually from 0.08 × 109 to 6.82 × 109 copies g−1 dry mass over time and correlated positively to the concentrations of methylated As. 16S rRNA gene sequencing and arsM clone library analysis confirmed the high abundance and diversity of arsM genes. Many of these genes were related to those from known As-methylating microbes, including Streptomyces sp., Amycolatopsis mediterranei and Sphaerobacter thermophiles. These results demonstrated that As methylation during manure composting is significant and, for the first time, established a linkage between As biomethylation and the abundance and diversity of the arsM functional genes in composting manure.
Absence of PD-L1 on tumor cells is associated with reduced MHC I expression and PD-L1 expression increases in recurrent serous ovarian cancer
Authors: Aust Stefanie , Felix Sophie , Auer Katharina , Bachmayr-Heyda Anna , Kenner Lukas , Dekan Sabine , Meier Samuel M. , Gerner Christopher , Grimm Christoph , Pils Dietmar
Published: March 2017

Immune-evasion and immune checkpoints are promising new therapeutic targets for several cancer entities. In ovarian cancer, the clinical role of programmed cell death receptor ligand 1 (PD-L1) expression as mechanism to escape immune recognition has not been clarified yet. We analyzed PD-L1 expression of primary ovarian and peritoneal tumor tissues together with several other parameters (whole transcriptomes of isolated tumor cells, local and systemic immune cells, systemic cytokines and metabolites) and compared PD-L1 expression between primary tumor and tumor recurrences. All expressed major histocompatibility complex (MHC) I genes were negatively correlated to PD-L1 abundances on tumor tissues, indicating two mutually exclusive immune-evasion mechanisms in ovarian cancer: either down-regulation of T-cell mediated immunity by PD-L1 expression or silencing of self-antigen presentation by down-regulation of the MHC I complex. In our cohort and in most of published evidences in ovarian cancer, low PD-L1 expression is associated with unfavorable outcome. Differences in immune cell populations, cytokines, and metabolites strengthen this picture and suggest the existence of concurrent pathways for progression of this disease. Furthermore, recurrences showed significantly increased PD-L1 expression compared to the primary tumors, supporting trials of checkpoint inhibition in the recurrent setting.
3D characterization of morphological changes in the intervertebral disc and endplate during aging: A propagation phase contrast synchrotron micro-tomography study
Authors: Cao Yong , Liao Shenghui , Zeng Hao , Ni Shuangfei , Tintani Francis , Hao Yongqiang , Wang Lei , Wu Tianding , Lu Hongbin , Duan Chunyue , Hu Jianzhong
Published: March 2017

A better understanding of functional changes in the intervertebral disc (IVD) and interaction with endplate is essential to elucidate the pathogenesis of IVD degeneration disease (IDDD). To date, the simultaneous depiction of 3D micro-architectural changes of endplate with aging and interaction with IVD remains a technical challenge. We aim to characterize the 3D morphology changes of endplate and IVD during aging using PPCST. The lumbar vertebral level 4/5 IVDs harvested from 15-day-, 4- and 24-month-old mice were initially evaluated by PPCST with histological sections subsequently analyzed to confirm the imaging efficiency. Quantitative assessments of age-related trends after aging, including mean diameter, volume fraction and connectivity of the canals, and endplate porosity and thickness, reached a peak at 4 months and significantly decreased at 24 months. The IVD volume consistently exhibited same trend of variation with the endplate after aging. In this study, PPCST simultaneously provided comprehensive details of 3D morphological changes of the IVD and canal network in the endplate and the interaction after aging. The results suggest that PPCST has the potential to provide a new platform for attaining a deeper insight into the pathogenesis of IDDD, providing potential therapeutic targets.
Two dimensional electrophysiological characterization of human pluripotent stem cell-derived cardiomyocyte system
Authors: Zhu Huanqi , Scharnhorst Kelsey S. , Stieg Adam Z. , Gimzewski James K. , Minami Itsunari , Nakatsuji Norio , Nakano Haruko , Nakano Atsushi
Published: March 2017

Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery. As human pluripotent stem cell-derived cardiomyocytes remain functionally fetal-type, close monitoring of electrophysiological maturation is critical for their further application to biology and translation. However, to date, electrophysiological analyses of stem cell-derived cardiomyocytes has largely been limited by biologically undefined factors including 3D nature of embryoid body, sera from animals, and the feeder cells isolated from mouse. Large variability in the aforementioned systems leads to uncontrollable and irreproducible results, making conclusive studies difficult. In this report, a chemically-defined differentiation regimen and a monolayer cell culture technique was combined with multielectrode arrays for accurate, real-time, and flexible measurement of electrophysiological parameters in translation-ready human cardiomyocytes. Consistent with their natural counterpart, amplitude and dV/dtmax of field potential progressively increased during the course of maturation. Monolayer culture allowed for the identification of pacemaking cells using the multielectrode array platform and thereby the estimation of conduction velocity, which gradually increased during the differentiation of cardiomyocytes. Thus, the electrophysiological maturation of the human pluripotent stem cell-derived cardiomyocytes in our system recapitulates in vivo development. This system provides a versatile biological tool to analyze human heart development, disease mechanisms, and the efficacy/toxicity of chemicals.
Plasmon-Modulated Excitation-Dependent Fluorescence from Activated CTAB Molecules Strongly Coupled to Gold Nanoparticles
Authors: Ding Si-Jing , Nan Fan , Liu Xiao-Li , Hao Zhong-Hua , Zhou Li , Zeng Jie , Xu Hong-Xing , Zhang Wei , Wang Qu-Quan
Published: March 2017

Excitation-dependent fluorophores (EDFs) have been attracted increasing attention owing to their high tunability of emissions and prospective applications ranging from multicolor patterning to bio-imaging. Here, we report tunable fluorescence with quenching dip induced by strong coupling of exciton and plasmon in the hybrid nanostructure of CTAB* EDFs and gold nanoparticles (AuNPs). The quenching dip in the fluorescence spectrum is tuned by adjusting excitation wavelength as well as plasmon resonance and concentration of AuNPs. The observed excitation-dependent emission spectra with quenching dip are theoretically reproduced and revealed to be induced by resonant energy transfer from multilevel EDFs with wider width channels to plasmonic AuNPs. These findings provide a new approach to prepare EDF molecules and a strategy to modulate fluorescence spectrum via exciton-to-plasmon energy transfer.
ACCORD: an assessment tool to determine the orientation of homodimeric coiled-coils
Authors: Kim Byeong-Won , Jung Yang Ouk , Kim Min Kyung , Kwon Do Hoon , Park Si Hoon , Kim Jun Hoe , Kuk Yong-Boo , Oh Sun-Joo , Kim Leehyeon , Kim Bong Heon , Yang Woo Seok , Song Hyun Kyu
Published: March 2017

The coiled-coil (CC) domain is a very important structural unit of proteins that plays critical roles in various biological functions. The major oligomeric state of CCs is a dimer, which can be either parallel or antiparallel. The orientation of each α-helix in a CC domain is critical for the molecular function of CC-containing proteins, but cannot be determined easily by sequence-based prediction. We developed a biochemical method for assessing differences between parallel and antiparallel CC homodimers and named it ACCORD (Assessment tool for homodimeric Coiled-Coil ORientation Decision). To validate this technique, we applied it to 15 different CC proteins with known structures, and the ACCORD results identified these proteins well, especially with long CCs. Furthermore, ACCORD was able to accurately determine the orientation of a CC domain of unknown directionality that was subsequently confirmed by X-ray crystallography and small angle X-ray scattering. Thus, ACCORD can be used as a tool to determine CC directionality to supplement the results of in silico prediction.
A rapid and visual turn-off sensor for detecting copper (II) ion based on DNAzyme coupled with HCR-based HRP concatemers
Authors: Xu Wentao , Tian Jingjing , Luo Yunbo , Zhu Longjiao , Huang Kunlun
Published: March 2017

To solve the requirement of on-site, rapid, and visual detection of copper (II) (Cu2+) in aqueous solution, a turn-off sensor for detecting copper (II) ion was developed based on Cu2+-dependent DNAzyme as the recognition element and hybridization chain reaction (HCR)-based horseradish peroxidase (HRP) concatemers as the signal amplifier and the signal report element. The detection unit, which was composed of the immobilized Cu2+-dependent DNAzyme coupled with HCR-based HRP concatemers via Waston-Crick base pairing, could catalyze hydrogen peroxide (H2O2) via TMB, generating obvious green color and turning yellow after sulfuric acid termination with optical absorption at 450 nm. Upon Cu2+ addition, the substrate strand of the Cu2+-dependent DNAzyme concatenated with the HCR-based HRP complex was irreversibly cleaved, efficiently causing dramatic reduction of the detection signal. Under optimal conditions, the detection signal decreased with the concentration of Cu2+ in 5 min, exhibiting a linear calibration from 0.05 to 3 μM with a detection limit of 8 nM. The sensor also displayed a high selectivity for Cu2+ given the specificity and anti-interference of the detection unit, and this system was applicable for monitoring Cu2+ in real water samples. Generally speaking, the proposed sensor exhibits good potential in environment surveys.
Mineral Surface Chemistry and Nanoparticle-aggregation Control Membrane Self-Assembly
Authors: Sahai Nita , Kaddour Hussein , Dalai Punam , Wang Ziqiu , Bass Garrett , Gao Min
Published: March 2017

The self-assembly of lipid bilayer membranes to enclose functional biomolecules, thus defining a “protocell,” was a seminal moment in the emergence of life on Earth and likely occurred at the micro-environment of the mineral-water interface. Mineral-lipid interactions are also relevant in biomedical, industrial and technological processes. Yet, no structure-activity relationships (SARs) have been identified to predict lipid self-assembly at mineral surfaces. Here we examined the influence of minerals on the self-assembly and survival of vesicles composed of single chain amphiphiles as model protocell membranes. The apparent critical vesicle concentration (CVC) increased in the presence of positively-charged nanoparticulate minerals at high loadings (mg/mL) suggesting unfavorable membrane self-assembly in such situations. Above the CVC, initial vesicle formation rates were faster in the presence of minerals. Rates were correlated with the mineral’s isoelectric point (IEP) and reactive surface area. The IEP depends on the crystal structure, chemical composition and surface hydration. Thus, membrane self-assembly showed rational dependence on fundamental mineral properties. Once formed, membrane permeability (integrity) was unaffected by minerals. Suggesting that, protocells could have survived on rock surfaces. These SARs may help predict the formation and survival of protocell membranes on early Earth and other rocky planets, and amphiphile-mineral interactions in diverse other phenomena.
A refined wideband acoustical holography based on equivalent source method
Authors: Ping Guoli , Chu Zhigang , Xu Zhongming , Shen Linbang
Published: March 2017

This paper is concerned with acoustical engineering and mathematical physics problem for the near-field acoustical holography based on equivalent source method (ESM-based NAH). An important mathematical physics problem in ESM-based NAH is to solve the equivalent source strength, which has multiple solving algorithms, such as Tikhonov regularization ESM (TRESM), iterative weighted ESM (IWESM) and steepest descent iteration ESM (SDIESM). To explore a new solving algorithm which can achieve better reconstruction performance in wide frequency band, a refined wideband acoustical holography (RWAH) is proposed. RWAH adopts IWESM below a transition frequency and switches to SDIESM above that transition frequency, and the principal components of input data in RWAH have been truncated. Further, the superiority of RWAH is verified by the comparison of comprehensive performance of TRESM, IWESM, SDIESM and RWAH. Finally, the experiments are conducted, confirming that RWAH can achieve better reconstruction performance in wide frequency band.
Vapour pressure deficit control in relation to water transport and water productivity in greenhouse tomato production during summer
Authors: Zhang Dalong , Du Qingjie , Zhang Zhi , Jiao Xiaocong , Song Xiaoming , Li Jianming
Published: March 2017

Although atmospheric vapour pressure deficit (VPD) has been widely recognized as the evaporative driving force for water transport, the potential to reduce plant water consumption and improve water productivity by regulating VPD is highly uncertain. To bridge this gap, water transport in combination with plant productivity was examined in tomato (Solanum lycopersicum L.) plants grown under contrasting VPD gradients. The driving force for water transport was substantially reduced in low-VPD treatment, which consequently decreased water loss rate and moderated plant water stress: leaf desiccation, hydraulic limitation and excessive negative water potential were prevented by maintaining water balance. Alleviation in water stress by reducing VPD sustained stomatal function and photosynthesis, with concomitant improvements in biomass and fruit production. From physiological perspectives, suppression of the driving force and water flow rate substantially reduced cumulative transpiration by 19.9%. In accordance with physiological principles, irrigation water use efficiency as criterions of biomass and fruit yield in low-VPD treatment was significantly increased by 36.8% and 39.1%, respectively. The reduction in irrigation was counterbalanced by input of fogging water to some extent. Net water saving can be increased by enabling greater planting densities and improving the evaporative efficiency of the mechanical system.
Integrative Diffusion-Weighted Imaging and Radiogenomic Network Analysis of Glioblastoma multiforme
Authors: Heiland Dieter Henrik , Simon-Gabriel Carl Philipp , Demerath Theo , Haaker Gerrit , Pfeifer Dietmar , Kellner Elias , Kiselev Valerij G. , Staszewski Ori , Urbach Horst , Weyerbrock Astrid , Mader Irina
Published: March 2017

In the past, changes of the Apparent Diffusion Coefficient in glioblastoma multiforme have been shown to be related to specific genes and described as being associated with survival. The purpose of this study was to investigate diffusion imaging parameters in combination with genome-wide expression data in order to obtain a comprehensive characterisation of the transcriptomic changes indicated by diffusion imaging parameters. Diffusion-weighted imaging, molecular and clinical data were collected prospectively in 21 patients. Before surgery, MRI diffusion metrics such as axial (AD), radial (RD), mean diffusivity (MD) and fractional anisotropy (FA) were assessed from the contrast enhancing tumour regions. Intraoperatively, tissue was sampled from the same areas using neuronavigation. Transcriptional data of the tissue samples was analysed by Weighted Gene Co-Expression Network Analysis (WGCNA) thus classifying genes into modules based on their network-based affiliations. Subsequent Gene Set Enrichment Analysis (GSEA) identified biological functions or pathways of the expression modules. Network analysis showed a strong association between FA and epithelial-to-mesenchymal-transition (EMT) pathway activation. Also, patients with high FA had a worse clinical outcome. MD correlated with neural function related genes and patients with high MD values had longer overall survival. In conclusion, FA and MD are associated with distinct molecular patterns and opposed clinical outcomes.
Selective induction of alternatively spliced FynT isoform by TNF facilitates persistent inflammatory responses in astrocytes
Authors: Lee Chingli , Low Clara Y. B. , Wong Siew Ying , Lai Mitchell K. P. , Tan Michelle G. K.
Published: March 2017

Fyn tyrosine kinase has been implicated in the pathogenesis of Alzheimer’s disease (AD). We have previously reported that upregulation of the FynT isoform in AD brains was partly associated with astrocyte activation. In this study, we demonstrated selective FynT induction in murine cortex and primary astrocyte culture after prolonged exposure to inflammatory stimulants, suggesting that FynT may mediate persistent neuroinflammation. To delineate the functional role of astrocytic FynT in association with TNF-mediated inflammatory responses, immortalized normal human astrocytes (iNHA) stably expressing FynT kinase constitutively active (FynT-CA) or kinase dead (FynT-KD) mutants were treated with TNF and compared for inflammatory responses using high-throughput real-time RT-PCR and Luminex multi-analyte immunoassays. FynT-CA but not FynT-KD mutant exhibited drastic induction of proinflammatory cytokines and chemokines after prolonged exposure to TNF, which could be attenuated by treating with Fyn kinase inhibitor PP2 or silencing via FynT-specific DsiRNA. FynT kinase activity-dependent induction of PKCδ expression, PKCδ phosphorylation, as well as NFκB activation was detected at the late phase but not the early phase of TNF signaling. In conclusion, selective FynT induction by TNF may facilitate persistent inflammatory responses in astrocytes, which is highly relevant to chronic neuroinflammation in neurodegenerative diseases including but not limited to AD.
ceRNA crosstalk stabilizes protein expression and affects the correlation pattern of interacting proteins
Authors: Martirosyan Araks , De Martino Andrea , Pagnani Andrea , Marinari Enzo
Published: March 2017

Gene expression is a noisy process and several mechanisms, both transcriptional and post-transcriptional, can stabilize protein levels in cells. Much work has focused on the role of miRNAs, showing in particular that miRNA-mediated regulation can buffer expression noise for lowly expressed genes. Here, using in silico simulations and mathematical modeling, we demonstrate that miRNAs can exert a much broader influence on protein levels by orchestrating competition-induced crosstalk between mRNAs. Most notably, we find that miRNA-mediated cross-talk (i) can stabilize protein levels across the full range of gene expression rates, and (ii) modifies the correlation pattern of co-regulated interacting proteins, changing the sign of correlations from negative to positive. The latter feature may constitute a potentially robust signature of the existence of RNA crosstalk induced by endogenous competition for miRNAs in standard cellular conditions.
Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice
Authors: Yu Yao , Li Shipeng , Wang Zhen , He Jindan , Ding Yijie , Zhang Haiming , Yu Wenli , Shi Yiwei , Cui Zilin , Wang Ximo , Wang Zhiliang , Sun Liying , Zhang Rongxin , Du Hongyin , Zhu Zhijun
Published: March 2017

Increasing evidence has linked autophagy to a detrimental role in hepatic ischemia- reperfusion (IR) injury (IRI). Here we focus on the role of interferon regulatory factor-1 (IRF-1) in regulating autophagy to aggravate hepatic IRI. We found that IRF-1 was up-regulated during hepatic IRI and was associated with an activation of the autophagic signaling. This increased IRF-1 expression, which was allied with high autophagic activity, amplified liver damage to IR, an effect which was abrogated by IRF-1 depletion. Moreover, IRF-1 contributed to P38 induced autophagic and apoptotic cell death, that can play a key role in liver dysfunction. The levels of P62 mRNA and protein were increased when P38 was activated and decreased when P38 was inhibited by SB203580. We conclude that IRF-1 functioned as a trigger to activate autophagy via P38 activation and that P62 was required for this P38-mediated autophagy. IRF-1 appears to exert a pivotal role in hepatic IRI, by predisposing hepatocytes to activate an autophagic pathway. Such an effect promotes autophagic cell death through the P38/P62 pathway. The identification of this novel pathway, that links expression levels of IRF-1 with autophagy, may provide new insights for the generation of novel protective therapies directed against hepatic IRI.
Percutaneous Irreversible Electroporation: Long-term survival analysis of 71 patients with inoperable malignant hepatic tumors
Authors: Niessen C. , Thumann S. , Beyer L. , Pregler B. , Kramer J. , Lang S. , Teufel A. , Jung E. M. , Stroszczynski C. , Wiggermann P.
Published: March 2017

Aim of this retrospective analysis was to evaluate the survival times after percutaneous irreversible electroporation (IRE) in inoperable liver tumors not amenable to thermal ablation. 71 patients (14 females, 57 males, median age 63.5 ± 10.8 years) with 103 liver tumors were treated in 83 interventions using IRE (NanoKnife® system). The median tumor short-axis diameter was 1.9 cm (minimum 0.4 cm, maximum 4.5 cm). 35 patients had primary liver tumors and 36 patients had liver metastases. The Kaplan-Meier method was employed to calculate the survival rates, and the different groups were compared using multivariate log-rank and Wilcoxon tests. The overall median survival time was 26.3 months; the median survival of patients with primary land secondary liver cancer did not significantly differ (26.8 vs. 19.9 months; p = 0.41). Patients with a tumor diameter >3 cm (p < 0.001) or more than 2 lesions (p < 0.005) died significantly earlier than patients with smaller or fewer tumors. Patients with hepatocellular carcinoma and Child-Pugh class B or C cirrhosis died significantly earlier than patients with Child-Pugh class A (p < 0.05). Patients with very early stage HCC survived significantly longer than patients with early stage HCC with a median survival of 22.3 vs. 13.7 months (p < 0.05).
Functional MRI in Awake Dogs Predicts Suitability for Assistance Work
Authors: Berns Gregory S. , Brooks Andrew M. , Spivak Mark , Levy Kerinne
Published: March 2017

The overall goal of this work was to measure the efficacy of fMRI for predicting whether a dog would be a successful service dog. The training and imaging were performed in 49 dogs entering service training at 17–21 months of age. 33 dogs completed service training and were matched with a person, while 10 were released for behavioral reasons (4 were selected as breeders and 2 were released for medical reasons.) After 2 months of training, fMRI responses were measured while each dog observed hand signals indicating either reward or no reward and given by both a familiar handler and a stranger. Using anatomically defined ROIs in the caudate, amygdala, and visual cortex, we developed a classifier based on the dogs’ subsequent training outcomes. The classifier had a positive predictive value of 94% and a negative predictive value of 67%. The area under the ROC curve was 0.91 (0.80 with 4-fold cross-validation, P = 0.01), indicating a significant predictive capability. The magnitude of response in the caudate was positively correlated with a successful outcome, while the response in the amygdala depended on the interaction with the visual cortex during the stranger condition and was negatively correlated with outcome (higher being associated with failure). These results suggest that, as indexed by caudate activity, successful service dogs generalize associations to hand signals regardless who gives them but without excessive arousal as measured in the amygdala.
Unusual seeding mechanism for enhanced performance in solid-phase magnetic extraction of Rare Earth Elements
Authors: Polido Legaria Elizabeth , Rocha Joao , Tai Cheuk-Wai , Kessler Vadim G. , Seisenbaeva Gulaim A.
Published: March 2017

Due to the increasing demand of Rare Earth Elements (REE or RE), new and more efficient techniques for their extraction are necessary, suitable for both mining and recycling processes. Current techniques such as solvent extraction or solid adsorbents entail drawbacks such as using big volumes of harmful solvents or limited capacity. Hybrid nanoadsorbents based on SiO2 and highly stable γ-Fe2O3-SiO2 nanoparticles, proved recently to be very attractive for adsorption of REE, yet not being the absolute key to solve the problem. In the present work, we introduce a highly appealing new approach in which the nanoparticles, rather than behaving as adsorbent materials, perform as inducers of crystallization for the REE in the form of hydroxides, allowing their facile and practically total removal from solution. This induced crystallization is achieved by tuning the pH, offering an uptake efficiency more than 20 times higher than previously reported (up to 900 mg RE3+/g vs. 40 mg RE3+/g). The obtained phases were characterized by SEM-EDS, TEM, STEM and EFTEM and 13C and 29Si solid state NMR. Magnetic studies showed that the materials possessed enough magnetic properties to be easily removed by a magnet, opening ways for an efficient and industrially applicable separation technique.
Abundant iron and sulfur oxidizers in the stratified sediment of a eutrophic freshwater reservoir with annual cyanobacterial blooms
Authors: Jin Long , Lee Chang Soo , Ahn Chi-Yong , Lee Hyung-Gwan , Lee Sanghyup , Shin Hyeon Ho , Lim Dhongil , Oh Hee-Mock
Published: March 2017

The microbial community in eutrophic freshwater sediment was investigated from a 67-cm-deep sediment core collected from the Daechung Reservoir in South Korea, where cyanobacterial blooms have occurred annually for the past 30 years. The majority of core sediments were characterized by dark-grayish, fine-grained mud with abundant gas-escaped and thinly laminated layers. Intervals of summer and winter seasons were represented by periodic peaks of geochemical profiles of parameters such as grain size and relative carbon mass ratios to various nutrients such as nitrogen, sulfur, and phosphorus. In bacteria, Proteobacteria (66.6%) was the most prevalent phylum, followed by Chloroflexi (8.9%), Bacteroidetes (5.1%), and Spirochaetes (2.6%). Archaea were also abundant, representing approximately half of the total prokaryotes in the sediments. Notably, three Bacteria (Sulfuricurvum, Sideroxydans, and Gallionella) and one Archaea (Thermoplasmata) accounted for 43.4% and 38.4% of the total bacteria and archaea, respectively, implying that iron and sulfur oxidizing microorganisms dominate in this eutrophic freshwater sediment. These results indicate that 1) eutrophic freshwater lakes in monsoon climates undergo a stratified sedimentary process with seasonal and annual variations in geochemical and microbial profiles, and 2) the microbial oxidative metabolism of iron and sulfur is notably active in sediments from a eutrophic lake.
Structural relaxation phenomena in silicate glasses modified by irradiation with femtosecond laser pulses
Authors: Seuthe Thomas , Mermillod-Blondin Alexandre , Grehn Moritz , Bonse Jörn , Wondraczek Lothar , Eberstein Markus
Published: March 2017

Structural relaxation phenomena in binary and multicomponent lithium silicate glasses were studied upon irradiation with femtosecond (fs) laser pulses (800 nm central wavelength, 130 fs pulse duration) and subsequent thermal annealing experiments. Depending on the annealing temperature, micro-Raman spectroscopy analyses evidenced different relaxation behaviours, associated to bridging and non-bridging oxygen structures present in the glass network. The results indicate that the mobility of lithium ions is an important factor during the glass modification with fs-laser pulses. Quantitative phase contrast imaging (spatial light interference microscopy) revealed that these fs-laser induced structural modifications are closely related to local changes in the refractive index of the material. The results establish a promising strategy for tailoring fs-laser sensitivity of glasses through structural mobility.
Heart rate responses induced by acoustic tempo and its interaction with basal heart rate
Authors: Watanabe Ken , Ooishi Yuuki , Kashino Makio
Published: March 2017

Many studies have revealed the influences of music on the autonomic nervous system (ANS). Since previous studies focused on the effects of acoustic tempo on the ANS, and humans have their own physiological oscillations such as the heart rate (HR), the effects of acoustic tempo might depend on the HR. Here we show the relationship between HR elevation induced by acoustic tempo and individual basal HR. Since high tempo-induced HR elevation requires fast respiration, which is based on sympatho-respiratory coupling, we controlled the participants’ respiration at a faster rate (20 CPM) than usual (15 CPM). We found that sound stimuli with a faster tempo than the individual basal HR increased the HR. However, the HR increased following a gradual increase in the acoustic tempo only when the extent of the gradual increase in tempo was within a specific range (around + 2%/min). The HR did not follow the increase in acoustic tempo when the rate of the increase in the acoustic tempo exceeded 3% per minute. These results suggest that the effect of the sympatho-respiratory coupling underlying the HR elevation caused by a high acoustic tempo depends on the basal HR, and the strength and the temporal dynamics of the tempo.
Surveillance strategies for Classical Swine Fever in wild boar – a comprehensive evaluation study to ensure powerful surveillance
Authors: Schulz Katja , Peyre Marisa , Staubach Christoph , Schauer Birgit , Schulz Jana , Calba Clémentine , Häsler Barbara , Conraths Franz J.
Published: March 2017

Surveillance of Classical Swine Fever (CSF) should not only focus on livestock, but must also include wild boar. To prevent disease transmission into commercial pig herds, it is therefore vital to have knowledge about the disease status in wild boar. In the present study, we performed a comprehensive evaluation of alternative surveillance strategies for Classical Swine Fever (CSF) in wild boar and compared them with the currently implemented conventional approach. The evaluation protocol was designed using the EVA tool, a decision support tool to help in the development of an economic and epidemiological evaluation protocol for surveillance. To evaluate the effectiveness of the surveillance strategies, we investigated their sensitivity and timeliness. Acceptability was analysed and finally, the cost-effectiveness of the surveillance strategies was determined. We developed 69 surveillance strategies for comparative evaluation between the existing approach and the novel proposed strategies. Sampling only within sub-adults resulted in a better acceptability and timeliness than the currently implemented strategy. Strategies that were completely based on passive surveillance performance did not achieve the desired detection probability of 95%. In conclusion, the results of the study suggest that risk-based approaches can be an option to design more effective CSF surveillance strategies in wild boar.
Global and regional changes in exposure to extreme heat and the relative contributions of climate and population change
Authors: Liu Zhao , Anderson Bruce , Yan Kai , Dong Weihua , Liao Hua , Shi Peijun
Published: March 2017

The frequency and intensity of extreme heat wave events have increased in the past several decades and are likely to continue to increase in the future under the influence of human-induced climate change. Exposure refers to people, property, systems, or other elements present in hazard zones that are thereby subject to potential losses. Exposure to extreme heat and changes therein are not just determined by climate changes but also population changes. Here we analyze output for three scenarios of greenhouse gas emissions and socio-economic growth to estimate future exposure change taking account of both climate and population factors. We find that for the higher emission scenario (RCP8.5-SSP3), the global exposure increases nearly 30-fold by 2100. The average exposure for Africa is over 118 times greater than it has been historically, while the exposure for Europe increases by only a factor of four. Importantly, in the absence of climate change, exposure is reduced by 75–95% globally and across all geographic regions, as compared with exposure under the high emission scenario. Under lower emission scenarios RCP4.5-SSP2 and RCP2.6-SSP1, the global exposure is reduced by 65% and 85% respectively, highlighting the efficacy of mitigation efforts in reducing exposure to extreme heat.
EEG in the classroom: Synchronised neural recordings during video presentation
Authors: Poulsen Andreas Trier , Kamronn Simon , Dmochowski Jacek , Parra Lucas C. , Hansen Lars Kai
Published: March 2017

We performed simultaneous recordings of electroencephalography (EEG) from multiple students in a classroom, and measured the inter-subject correlation (ISC) of activity evoked by a common video stimulus. The neural reliability, as quantified by ISC, has been linked to engagement and attentional modulation in earlier studies that used high-grade equipment in laboratory settings. Here we reproduce many of the results from these studies using portable low-cost equipment, focusing on the robustness of using ISC for subjects experiencing naturalistic stimuli. The present data shows that stimulus-evoked neural responses, known to be modulated by attention, can be tracked for groups of students with synchronized EEG acquisition. This is a step towards real-time inference of engagement in the classroom.