Significance of cytokine profile in renal complications in children during the COVID-19 pandemic: An observational cohort retrospective clinical study
Authors: Axmedjanova N. I. 
Published: February 2025

Background. Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) represents a new global health challenge, potentially affecting all organs, including the kidneys. Renal pathology has been observed more frequently in younger children and individuals with comorbidities. Acute kidney injury has been reported in some patients with urinary tract pathology triggered by COVID-19 at the time of hospital discharge.

Objectives. To investigate critical aspects of the cytokine profile in serum and urine of children aged 9 to 14 years with acute pyelonephritis, both in the context of COVID-19 and without it.

Methods. The observational cohort retrospective clinical study involved 28 children diagnosed with acute pyelonephritis from the Nephrology Department, Samarkand Regional Children’s Multidisciplinary Medical Center, Uzbekistan. The control group consisted of 14 healthy children undergoing routine medical examinations according to their age and/or seeking medical advice in the outpatient clinic. Laboratory analyses of biological samples were conducted at the clinical diagnostic laboratory of the institution where the children were treated and monitored, as well as at international multidisciplinary medical center Innova Expert (Samarkand) and the Central Scientific Research Laboratory of the Institute of Immunology and Human Genomics, along with the laboratory of the Gunchamed clinic (Tashkent). Participants were selected for the study from January 2021 to December 2022. Children with acute pyelonephritis were included in the study upon hospitalization and were divided into two groups based on their COVID-19 history: Group 1 (n = 14) consisted of children with acute pyelonephritis without a history of COVID-19, while Group 2 (n = 14) included patients recovering from COVID-19 with a convalescent period ranging from 3 weeks to 3 months. The primary outcome consisted in the assessment of the cytokine profile (interleukin-4, interleukin-6, tumor necrosis factor-alpha, and interferon-gamma) in serum and urine. A secondary outcome comprised the evaluation of renal function based on the condition of proximal and distal tubules. Statistical analysis of laboratory results was performed using Excel (Microsoft Office, 2016, USA) and StatPlus version 7 (AnalystSoft Inc., USA). The differences were considered statistically significant at p < 0.05.

Results. A statistically significant increase in the concentrations of interleukin-6, interleukin-4, tumor necrosis factor-alpha, and interferon-gamma was observed in urine samples. In Group 1, tumor necrosis factor-alpha levels were found to be 4.9 times higher and interferon-gamma levels were 11.0 times higher compared to the control group. In Group 2, these values exceeded those of healthy children by 8.8 and 14.8 times, respectively. Additionally, interleukin-4 levels in urine exceeded those of the control group by 4.7 times for Group 1 and by 5.6 times for Group 2. The analysis results indicate a significant role of interleukin-6 as an aggressive factor in the development of tubulointerstitial renal diseases, with its level in urine being increased by 37.9 times in children from Group 1 and by 47.1 times in patients from Group 2 compared to healthy children. A substantial growth in interleukin-6 levels even with less pronounced tubulointerstitial injury and a rise in its values as the tubulointerstitial injury grows suggest an increased cytokine excretion in urine due to tubular function impairment caused by SARS-CoV-2, leading to proximal tubular damage.

Conclusion. Controlling the course of latent lesions of tubular functions (screening study) in patients who have undergone COVID-19 implies studying the level of cytokines (interleukin-6, interferon-γ, tumor necrosis factor-α, interleukin-4) in urine, in order to determine the degree of proliferative changes in the tubulointerstitial tissue of the kidneys and forming risk groups of patients for chronicity of the process.