bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Studies on Mg-Zn-Sn-Based Alloys Developed as a New Kind of Biodegradable Metal
Authors: Wen Yafeng , Liu Qingshan , Zhao Weikang , Yang Qiming , Wang Jingfeng , Jiang Dianming
Published: March 2021

Mg-Zn-Sn-based alloys are widely used in the industrial field because of their low-cost, high-strength and heat-resistant characteristics. However, their application in the biomedical field has been rarely reported. In the present study, biodegradable Mg-1Zn-1Sn and Mg-1Zn-1Sn-0.2Sr alloys were fabricated. Their microstructure, surface characteristics, mechanical properties and bio-corrosion properties were carried out using an optical microscope (OM), X-ray diffraction (XRD), electron microscopy (SEM), mechanical testing, electrochemical and immersion test. The cell viability and morphology were studied by cell counting kit-8 (CCK-8) assay, live/dead cell assay, confocal laser scanning microscopy (CLSM) and SEM. The osteogenic activity was systematically investigated by alkaline phosphatase (ALP) assay, Alizarin Red S (ARS) staining, immunofluorescence staining and quantitative real time-polymerase chain reaction (qRT-PCR). The results showed that a small amount of strontium (Sr) (0.2 wt.%) significantly enhanced the corrosion resistance of the Mg-1Zn-1Sn alloy by grain refinement and decreasing the corrosion current density. Meanwhile, the mechanical properties were also improved via the second phase strengthening. Both Mg-1Zn-1Sn and Mg-1Zn-1Sn-0.2Sr alloys showed excellent biocompatibility, significantly promoted cell proliferation, adhesion and spreading. Particularly, significant increases in ALP activity, ARS staining, type I collagen (COL-I) expression as well as the expressions of three osteogenesis-related genes (runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (Bglap)) were observed for the Mg-1Zn-1Sn-0.2Sr group. In summary, this study demonstrated that Mg-Zn-Sn-based alloy has great application potential in orthopedics and Sr is an ideal alloying element of Mg-Zn-Sn-based alloy, which optimizes its corrosion resistance, mechanical properties and osteoinductive activity.
The Effect of Size and Thermal Treatment on the Photoluminescent Properties of Europium-Doped SiO2 Nanoparticles Prepared in One Pot by Sol-Gel
Authors: Fneich Hussein , Gaumer Nathalie , Chaussedent Stéphane , Mehdi Ahmad , Blanc Wilfried
Published: March 2021

Europium (Eu)-doped silica nanoparticles have attracted great interest for different applications, in particular in biomedicine as biosensors or for tissue regeneration. Sol-gel is the most common process used to prepare those particles, with size varying from tens to hundreds of nanometers. In this article, we focus our attention on the comparison between two commonly used sol-gel derived methods: reverse microemulsion (for particles smaller than 100 nm) and Stöber method (for particles larger than 100 nm). Europium concentration was varied between 0.2 and 1 mol%, and the nanoparticle diameters were 10, 50 and 100 nm. The link between the local environment of europium ions and their optical properties was investigated and discussed. Using Transmission Electron Microscopy, nitrogen sorption, X-ray diffraction, Fourier-Transform Infra-Red and pulsed doubled Nd:YAG laser, we confirmed that fluorescence lifetime was improved by thermal treatment at 900 °C due to the elimination of aqueous environment and modification of structure disorder. The size of nanoparticles, the amount of europium and the thermal treatment of obtained materials influence the emission spectra and the decay curves of Eu3+.
The Effect of SCMs in Blended Cements on Sorption Characteristics of Superabsorbent Polymers
Authors: Rostami Rohollah , Klemm Agnieszka J. , Almeida Fernando C. R.
Published: March 2021

Supplementary cementitious materials (SCMs), such as fly ash (FA) and ground granulated blast-furnace slag (GGBS), are often used as a partial replacement of cements to improve the sustainability of Portland cement-based materials and reduce their environmental impact. Superabsorbent polymers (SAPs) can be successfully used as internal curing agents in ultra-high performance cementitious materials by facilitating the hydration process and controlling the water supply in both fresh and hardened states. This paper intends to characterise the physical and chemical properties of SAPs and their sorption properties in different blended cement environments. The swelling capacity and kinetics of absorption of three superabsorbent polymers with different chemical compositions and grading were tested in different cement environments. Experimental results of their sorption performance in distinct solutions, including deionised water (DI), Portland cement (PC), and blended cements (PC-FA and PC-GGBS) and changes in pH of different solutions over time were investigated. The results showed that PC-FA solution had the lowest pH followed by PC-GGBS solution. Moreover, SAPs samples displayed the highest absorption capacities in PC-FA solutions, and the lowest swelling capacities were found in PC-GGBS solutions. Furthermore, SAP with smaller particle sizes had the greatest absorption capacity values in all solutions.
A Family of Functionally-Fitted Third Derivative Block Falkner Methods for Solving Second-Order Initial-Value Problems with Oscillating Solutions
Authors: Ramos Higinio , Abdulganiy Ridwanulahi , Olowe Ruth , Jator Samuel
Published: March 2021

One of the well-known schemes for the direct numerical integration of second-order initial-value problems is due to Falkner. This paper focuses on the construction of a family of adapted block Falkner methods which are frequency dependent for the direct numerical solution of second-order initial value problems with oscillatory solutions. The techniques of collocation and interpolation are adopted here to derive the new methods. The study of the properties of the proposed adapted block Falkner methods reveals that they are consistent and zero-stable, and thus, convergent. Furthermore, the stability analysis and the algebraic order conditions of the proposed methods are established. As may be seen from the numerical results, the resulting family is efficient and competitive compared to some recent methods in the literature.
On Time-Periodic Bifurcation of a Sphere Moving under Gravity in a Navier-Stokes Liquid
Authors: Galdi Giovanni P.
Published: March 2021

We provide sufficient conditions for the occurrence of time-periodic Hopf bifurcation for the coupled system constituted by a rigid sphere, S, freely moving under gravity in a Navier-Stokes liquid. Since the region of flow is unbounded (namely, the whole space outside S), the main difficulty consists in finding the appropriate functional setting where general theory may apply. In this regard, we are able to show that the problem can be formulated as a suitable system of coupled operator equations in Banach spaces, where the relevant operators are Fredholm of index 0. In such a way, we can use the theory recently introduced by the author and give sufficient conditions for time-periodic bifurcation to take place.
Marine-Derived Macrolides 1990–2020: An Overview of Chemical and Biological Diversity
Authors: Zhang Hairong , Zou Jiabin , Yan Xiaoxue , Chen Junlong , Cao Xiujiao , Wu Jialing , Liu Yinghui , Wang Tingting
Published: March 2021

Macrolides are a significant family of natural products with diverse structures and bioactivities. Considerable effort has been made in recent decades to isolate additional macrolides and characterize their chemical and bioactive properties. The majority of macrolides are obtained from marine organisms, including sponges, marine microorganisms and zooplankton, cnidarians, mollusks, red algae, bryozoans, and tunicates. Sponges, fungi and dinoflagellates are the main producers of macrolides. Marine macrolides possess a wide range of bioactive properties including cytotoxic, antibacterial, antifungal, antimitotic, antiviral, and other activities. Cytotoxicity is their most significant property, highlighting that marine macrolides still encompass many potential antitumor drug leads. This extensive review details the chemical and biological diversity of 505 macrolides derived from marine organisms which have been reported from 1990 to 2020.
The Assessment of Endothelial Dysfunction among OSA Patients after CPAP Treatment
Authors: Brożyna-Tkaczyk Klaudia , Myśliński Wojciech , Mosiewicz Jerzy
Published: March 2021

Background and Objectives: Microcirculation dysfunction is present in patients with obstructive sleep apnea (OSA). Intermittent hypoxia generates “oxidative stress”, which contributes to chronic inflammation. The secretion of nitric oxide (NO), which is responsible for adequate regulation of the endothelium, is impaired due to a decrease in endothelial nitric oxide synthetase (eNOS) expression and an increase in endogenous eNOS inhibitors. Furthermore, nocturnal awakenings lead to the dysregulation of cortisol release and increased stimulation of the sympathetic nervous system. The non-invasive method of choice in OSA treatment is continuous positive airway pressure (CPAP). Materials and Methods: PubMed, Scopus, and Google Scholar databases were searched, and only papers published in the last 15 years were subsequently analyzed. For this purpose, we searched for keywords in article titles or contents such as “obstructive sleep apnea”, “microcirculation”, and “CPAP”. In our review, we only studied English articles that reported systemic reviews and meta-analyses, clinical studies, and case reports. Results: Endothelial dysfunction can be assessed by methods based on reactive hyperemia, such as flow-mediated dilation (FMD) measured by ultrasonography, laser-Doppler flowmetry (LDF), or capillaroscopy. In invasive techniques, intravenous administration of vasodilator substances takes place. Some surveys detected impaired microcirculation in OSA patients compared with healthy individuals. The level of dysfunction depended on the severity of OSA. CPAP treatment significantly improved endothelial function and microvascular blood flow and lowered the inflammatory mediator level. Conclusions: The first-choice treatment—CPAP—reduces the number of apneas and hypopneas during the night, induces the reversal of hypopnea and the chronic inflammatory state, and enhances activation of the sympathetic nervous system. Changes are visible as improved blood flow in both macro- and microcirculation, increased arterial elasticity, and decreased stiffness. Thus, early implementation of adequate treatment could be essential to reduce high cardiovascular risk in patients with OSA.
Investigating the Safety and Efficacy of Platelet-Rich Plasma (PRP) Treatment for Female Androgenetic Alopecia: Review of the Literature
Authors: Mercuri Santo Raffaele , Paolino Giovanni , Di Nicola Matteo Riccardo , Vollono Laura
Published: March 2021

Background: female androgenetic alopecia (FAGA) is a common cause of non-scarring alopecia in women, affecting approximately 40% of women by age 50, bearing a significant psychosocial burden on affected patients. Platelet-rich plasma (PRP) has been widely investigated as a potential effective treatment for several dermatological conditions, including male androgenetic alopecia (MAGA). However, few studies have been conducted focusing on the use of PRP in FAGA. The aim of this review was to identify reports that investigated the use of PRP for the treatment of FAGA. Methods: Electronic databases of MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL) from inception to September 2020 have been searched using different combinations of the following terms: “androgenetic alopecia,” “FAGA,” “female pattern hair loss,” “platelet-rich fibrin,” “platelet-rich plasma,” and “PRP”. Results and conclusions: Eight (n = 8) clinical studies consistent with our research were identified. A total of 197 subjects has been enrolled in the included studies. All of them were adult female patients (mean age: 38.9) affected by female pattern hair loss. PRP is a well-tolerated procedure which showed promising results in males-only and mixed populations of AGA patients. PRP showed to produce high levels of satisfaction and improvement in the quality of life in patients affected by FAGA. In the light of this evidence, PRP may be proposed in patients who did not respond or did not tolerate topical minoxidil, as well as in combination with topical and oral treatments.
New Science Based Concepts for Increased Efficiency in Battery Recycling
Authors: Friedrich Bernd , Schwich Lilian
Published: March 2021

It is a common understanding worldwide that electromobility will have a significant share in passenger transport and that there will be a very dynamic increase in the return volumes of discarded batteries in the future [...]
Process Monitoring in Friction Stir Welding Using Convolutional Neural Networks
Authors: Hartl Roman , Bachmann Andreas , Habedank Jan Bernd , Semm Thomas , Zaeh Michael F.
Published: March 2021

Preliminary studies have shown the superiority of convolutional neural networks (CNNs) compared to other network architectures for determining the surface quality of friction stir welds. In this paper, CNNs were employed to detect cavities inside friction stir welds by evaluating inline measured process data. The aim was to determine whether CNNs are suitable for identifying surface defects exclusively, or if the approach is transferable to internal weld defects. For this purpose, 120 welds were produced and examined by ultrasonic testing, which was the basis for labeling the data as “good” or “defective.” Different types of artificial neural network were tested for predicting the placement of the welds into the defined classes. It was found that the way of labeling the data is significant for the accuracy achievable. When the complete welds were uniformly labeled as “good” or “defective,” an accuracy of 98.5% was achieved by a CNN, which was a significant improvement compared to the state of the art. When the welds were labeled segment-wise, an accuracy of 79.2% was obtained by using a CNN, showing that a segment-wise prediction of the cavities is also possible. The results confirm that CNNs are well suited for process monitoring in friction stir welding and their application enables the identification of various defect types.
Disposable Pencil Graphite Electrode for Diosmin Voltammetric Analysis
Authors: David Iulia Gabriela , Oancea Alexandra-Gabriela , Buleandră Mihaela , Popa Dana Elena , Iorgulescu Emilia Elena , Ciobanu Adela Magdalena
Published: March 2021

Diosmin (DIO) is a naturally occurring flavonoid with multiple beneficial effects on human health. The presence of different hydroxyl groups in diosmin structure enables its electrochemical investigation and quantification. This work presents, for the first time, diosmin voltammetric behavior and quantification on the cost-effective, disposable pencil graphite electrode (PGE). Diosmin oxidation on PGE involves two irreversible steps, generating products with reversible redox behaviors. All electrode processes are pH-dependent and predominantly adsorption-controlled. Differential pulse (DPV) and adsorptive stripping differential pulse (AdSDPV) voltammetric methods have been optimized for diosmin quantification o an H-type PGE, in 0.100 mol/L H2SO4. The linear ranges and limits of detection were for DPV 1.00 × 10−6–1.00 × 10−5 mol/L and 2.76 × 10−7 mol/L DIO for DPV and 1.00 × 10−7–2.50 × 10−6 mol/L and 7.42 × 10−8 mol/L DIO for AdSDPV, respectively. The DPV method was successfully applied for diosmin quantification in dietary supplement tablets. The percentage recovery was 99.87 ± 4.88%.
Deformation of an Encapsulated Leukemia HL60 Cell through Sudden Contractions of a Microfluidic Channel
Authors: Nooranidoost Mohammad , Kumar Ranganathan
Published: March 2021

Migration of an encapsulated leukemia HL60 cell through sudden contractions in a capillary tube is investigated. An HL60 cell is initially encapsulated in a viscoelastic shell fluid. As the cell-laden droplet moves through the sudden contraction, shear stresses are experienced around the cell. These stresses along with the interfacial force and geometrical effects cause mechanical deformation which may result in cell death. A parametric study is done to investigate the effects of shell fluid relaxation time, encapsulating droplet size and contraction geometries on cell mechanical deformation. It is found that a large encapsulating droplet with a high relaxation time will undergo low cell mechanical deformation. In addition, the deformation is enhanced for capillary tubes with narrow and long contraction. This study can be useful to characterize cell deformation in constricted microcapillaries and to improve cell viability in bio-microfluidics.
Pain-Administrable Neuron Electrode with Wireless Energy Transmission: Architecture Design and Prototyping
Authors: Lin Chin-Yu , Chang Li-Chi , Chen Jyh-Chern , Chen Meng-Sheng , Yu Hsun , Wang Mei-Chih
Published: March 2021

Back pain resulted from spine disorders reaches 60–80% prevalence in humans, which seriously influences life quality and retards economic production. Conventional electrical pain relief therapy uses radiofrequency to generate a high temperature of 70–85 °C on the electrode tip to destroy the neural transmission and stop the pain. However, due to the larger area of stimulation, eliciting significant side effects, such as paralysis, contraction, and a slightly uncomfortable feeling, our study aimed to design a tiny and stretchable neural stimulatory electrode that could be precisely anchored adjacent to the dorsal root ganglion which needs therapy and properly interfere with the sensory neural transmission. We also designed a subcutaneously implantable wireless power transmission (WPT) device to drive the neural stimulatory electrode. Through the study, we elaborated the design concept and clinical problems, and achieved: (1) the architecture design and simulation of the transdermal wireless power transferred device, (2) a wrap-able pulsed radiofrequency (PRF) stimulatory electrode, (3) an insulation packaging design of the titanium protection box. The feasibility study and hands-on prototype were also carried out.
Technological and Safety Attributes of Lactic Acid Bacteria and Yeasts Isolated from Spontaneously Fermented Greek Wheat Sourdoughs
Authors: Syrokou Maria K. , Tziompra Sofia , Psychogiou Eleni-Efthymia , Mpisti Sofia-Despoina , Paramithiotis Spiros , Bosnea Loulouda , Mataragas Marios , Skandamis Panagiotis N. , Drosinos Eleftherios H.
Published: March 2021

The aim of the present study was to assess the technological and safety potential of 207 lactic acid bacteria (LAB) and 195 yeast strains isolated from spontaneously fermented Greek wheat sourdoughs. More accurately, the amylolytic, proteolytic, lipolytic, phytase and amino acid decarboxylase activities, along with the production of exopolysaccharides and antimicrobial compounds by the LAB and yeast isolates, were assessed. A well diffusion assay revealed seven proteolytic LAB and eight yeast strains; hydrolysis of tributyrin was evident only in 11 LAB strains. A further Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) indicated partial hydrolysis of gluten. Lipolysis kinetics over 21 days was applied, exhibiting that lipolytic activity ranged from 6.25 to 65.50 AU/mL. Thirteen LAB inhibited Penicillium olsonii and Aspergillus niger growth and 12 yeast strains inhibited Pe. chrysogenum growth. Twenty-one Lactiplantibacillus plantarum strains exhibited inhibitory activity against Listeria monocytogenes, as well as several sourdough-associated isolates. The structural gene encoding plantaricin 423 was detected in 19 Lcb. plantarum strains, while the structural genes encoding plantaricins NC8, PlnE/F, PlnJ/K, and S were detected in two Lcb. plantarum strains. None of the microbial strains tested exhibited exopolysaccharide (EPS) production, amino acid decarboxylase, amylolytic or phytase activity. The technological and safety potential of the Lcb. plantarum and Wickerhamomyces anomalus strains was highlighted, since some of them exhibited proteolytic, lipolytic, antibacterial and antimould activities.
Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
Authors: Leon Fanny , Pinchon Elena , Temurok Nevzat , Morvan François , Vasseur Jean-Jacques , Clot Martine , Foulongne Vincent , Cantaloube Jean-François , Vande Perre Philippe , Molès Jean-Pierre , Daynès Aurélien , Fournier-Wirth Chantal
Published: March 2021

Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
Highly Porous Carbons Synthesized from Tannic Acid via a Combined Mechanochemical Salt-Templating and Mild Activation Strategy
Authors: Głowniak Sylwia , Szczęśniak Barbara , Choma Jerzy , Jaroniec Mietek
Published: March 2021

Highly porous activated carbons were synthesized via the mechanochemical salt-templating method using both sustainable precursors and sustainable chemical activators. Tannic acid is a polyphenolic compound derived from biomass, which, together with urea, can serve as a low-cost, environmentally friendly precursor for the preparation of efficient N-doped carbons. The use of various organic and inorganic salts as activating agents afforded carbons with diverse structural and physicochemical characteristics, e.g., their specific surface areas ranged from 1190 m2·g−1 to 3060 m2·g−1. Coupling the salt-templating method and chemical activation with potassium oxalate appeared to be an efficient strategy for the synthesis of a highly porous carbon with a specific surface area of 3060 m2·g−1, a large total pore volume of 3.07 cm3·g−1 and high H2 and CO2 adsorption capacities of 13.2 mmol·g−1 at −196 °C and 4.7 mmol·g−1 at 0 °C, respectively. The most microporous carbon from the series exhibited a CO2 uptake capacity as high as 6.4 mmol·g−1 at 1 bar and 0 °C. Moreover, these samples showed exceptionally high thermal stability. Such activated carbons obtained from readily available sustainable precursors and activators are attractive for several applications in adsorption and catalysis.
Expanding the Averievite Family, (MX)Cu5O2(T5+O4)2 (T5+ = P, V; M = K, Rb, Cs, Cu; X = Cl, Br): Synthesis and Single-Crystal X-ray Diffraction Study
Authors: Kornyakov Ilya V. , Vladimirova Victoria A. , Siidra Oleg I. , Krivovichev Sergey V.
Published: March 2021

Averievite-type compounds with the general formula (MX)[Cu5O2(TO4)], where M = alkali metal, X = halogen and T = P, V, have been synthesized by crystallization from gases and structurally characterized for six different compositions: 1 (M = Cs; X = Cl; T = P), 2 (M = Cs; X = Cl; T = V), 3 (M = Rb; X = Cl; T = P), 4 (M = K; X = Br; T = P), 5 (M = K; X = Cl; T = P) and 6 (M = Cu; X = Cl; T = V). The crystal structures of the compounds are based upon the same structural unit, the layer consisting of a kagome lattice of Cu2+ ions and are composed from corner-sharing (OCu4) anion-centered tetrahedra. Each tetrahedron shares common corners with three neighboring tetrahedra, forming hexagonal rings, linked into the two-dimensional [O2Cu5]6+ sheets parallel to (001). The layers are interlinked by (T5+O4) tetrahedra (T5+ = V, P) attached to the bases of the oxocentered tetrahedra in a “face-to-face” manner. The resulting electroneutral 3D framework {[O2Cu5](T5+O4)2}0 possesses channels occupied by monovalent metal cations M+ and halide ions X−. The halide ions are located at the centers of the hexagonal rings of the kagome nets, whereas the metal cations are in the interlayer space. There are at least four different structure types of the averievite-type compounds: the P-3m1 archetype, the 2 × 2 × 1 superstructure with the P-3 space group, the monoclinically distorted 1 × 1 × 2 superstructure with the C2/c symmetry and the low-temperature P21/c superstructure with a doubled unit cell relative to the high-temperature archetype. The formation of a particular structure type is controlled by the interplay of the chemical composition and temperature. Changing the chemical composition may lead to modification of the structure type, which opens up the possibility to tune the geometrical parameters of the kagome net of Cu2+ ions.
Chemokine Receptor 5 Antagonism Causes Reduction in Joint Inflammation in a Collagen-Induced Arthritis Mouse Model
Authors: Ansari Mushtaq A. , Nadeem Ahmed , Bakheet Saleh A. , Attia Sabry M. , Shahid Mudassar , Alyousef Faris S. , Alswailem Mohammed A. , Alqinyah Mohammed , Ahmad Sheikh F.
Published: March 2021

Rheumatoid arthritis (RA) is a chronic inflammatory disease mainly affecting the synovial joints. A highly potent antagonist of C-C chemokine receptor 5 (CCR5), maraviroc (MVC), plays an essential role in treating several infectious diseases but has not yet been evaluated for its potential effects on RA development. This study focused on evaluating the therapeutic potential of MVC on collagen-induced arthritis (CIA) in DBA/1J mice. Following CIA induction, animals were treated intraperitoneally with MVC (50 mg/kg) daily from day 21 until day 35 and evaluated for clinical score and histopathological changes in arthritic inflammation. We further investigated the effect of MVC on Th9 (IL-9, IRF-4, and GATA3) and Th17 (IL-21R, IL-17A, and RORγT) cells, TNF-α, and RANTES in CD8+ T cells in the spleen using flow cytometry. We also assessed the effect of MVC on mRNA and protein levels of IL-9, IL-17A, RORγT, and GATA3 in knee tissues using RT-PCR and western blot analysis. MVC treatment in CIA mice attenuated the clinical and histological severity of inflammatory arthritis, and it substantially decreased IL-9, IRF4, IL-21R, IL-17A, RORγT, TNF-α, and RANTES production but increased GATA3 production in CD8+ T cells. We further observed that MVC treatment decreased IL-9, IL-17A, and RORγt mRNA and protein levels and increased those of GATA3. This study elucidates the capacity of MVC to ameliorate the clinical and histological signs of CIA by reducing pro-inflammatory responses, suggesting that MVC may have novel therapeutic uses in the treatment of RA.
Superoxide Dismutase Administration: A Review of Proposed Human Uses
Authors: Rosa Arianna Carolina , Corsi Daniele , Cavi Niccolò , Bruni Natascia , Dosio Franco
Published: March 2021

Superoxide dismutases (SODs) are metalloenzymes that play a major role in antioxidant defense against oxidative stress in the body. SOD supplementation may therefore trigger the endogenous antioxidant machinery for the neutralization of free-radical excess and be used in a variety of pathological settings. This paper aimed to provide an extensive review of the possible uses of SODs in a range of pathological settings, as well as describe the current pitfalls and the delivery strategies that are in development to solve bioavailability issues. We carried out a PubMed query, using the keywords “SOD”, “SOD mimetics”, “SOD supplementation”, which included papers published in the English language, between 2012 and 2020, on the potential therapeutic applications of SODs, including detoxification strategies. As highlighted in this paper, it can be argued that the generic antioxidant effects of SODs are beneficial under all tested conditions, from ocular and cardiovascular diseases to neurodegenerative disorders and metabolic diseases, including diabetes and its complications and obesity. However, it must be underlined that clinical evidence for its efficacy is limited and consequently, this efficacy is currently far from being demonstrated.
Modifications of Plasma Membrane Organization in Cancer Cells for Targeted Therapy
Authors: Choromańska Anna , Chwiłkowska Agnieszka , Kulbacka Julita , Baczyńska Dagmara , Rembiałkowska Nina , Szewczyk Anna , Michel Olga , Gajewska-Naryniecka Agnieszka , Przystupski Dawid , Saczko Jolanta
Published: March 2021

Modifications of the composition or organization of the cancer cell membrane seem to be a promising targeted therapy. This approach can significantly enhance drug uptake or intensify the response of cancer cells to chemotherapeutics. There are several methods enabling lipid bilayer modifications, e.g., pharmacological, physical, and mechanical. It is crucial to keep in mind the significance of drug resistance phenomenon, ion channel and specific receptor impact, and lipid bilayer organization in planning the cell membrane-targeted treatment. In this review, strategies based on cell membrane modulation or reorganization are presented as an alternative tool for future therapeutic protocols.
Size-Dependent Electroluminescence and Current-Voltage Measurements of Blue InGaN/GaN µLEDs down to the Submicron Scale
Authors: Wolter Stefan , Spende Hendrik , Gülink Jan , Hartmann Jana , Wehmann Hergo-Heinrich , Waag Andreas , Lex Andreas , Avramescu Adrian , Lugauer Hans-Jürgen , Malm Norwin von , Drolet Jean-Jacques , Strassburg Martin
Published: March 2021

Besides high-power light-emitting diodes (LEDs) with dimensions in the range of mm, micro-LEDs (μLEDs) are increasingly gaining interest today, motivated by the future applications of μLEDs in augmented reality displays or for nanometrology and sensor technology. A key aspect of this miniaturization is the influence of the structure size on the electrical and optical properties of μLEDs. Thus, in this article, investigations of the size dependence of the electro-optical properties of μLEDs, with diameters in the range of 20 to 0.65 μm, by current–voltage and electroluminescence measurements are described. The measurements indicated that with decreasing size leakage currents in the forward direction decrease. To take advantage of these benefits, the surface has to be treated properly, as otherwise sidewall damages induced by dry etching will impair the optical properties. A possible countermeasure is surface treatment with a potassium hydroxide based solution that can reduce such defects.
‘It’s Hard to Make Good Choices and It Costs More’: Adolescents’ Perception of the External School Food Environment
Authors: Kelly Colette , Callaghan Mary , Gabhainn Saoirse Nic
Published: March 2021

Research on the impact of school and community food environments on adolescent food choice is heavily reliant on objective rather than subjective measures of food outlets around schools and homes. Gaining the perspective of adolescents and how they perceive and use food environments is needed. The aim of this study was to explore adolescent’s perception and use of the food environment surrounding their schools. Purposive sampling was used to recruit schools. Mapping exercises and discussion groups were facilitated with 95 adolescents from six schools. Thematic analysis showed that adolescents are not loyal to particular shops but are attracted to outlets with price discounts, those with ‘deli’ counters and sweets. Cost, convenience and choice are key factors influencing preference for food outlets and foods. Quality, variety and health were important factors for adolescents but these features, especially affordable healthy food, were hard to find. Social factors such as spending time with friends is also an important feature of food environments that deserves further attention. Adolescents’ perceptions of their food environment provide insights into features that can be manipulated to enable healthy choices.
Heterogeneities in Consumer Diet Quality and Health Outcomes of Consumers by Store Choice and Income
Authors: Dhakal Chandra K. , Khadka Savin
Published: March 2021

Obesity and other diet-related health conditions have received much attention in the public health literature over the past two decades. This study investigates the relationship between household food budget shares at different food outlets with diet quality and weight-related health outcomes in the United States. Our analysis used event-level food purchase data from the national household food acquisition and purchases survey (FoodAPS). We find that, after controlling for observables, food purchase location is significantly associated with diet quality and body mass index (BMI). Our findings indicate that larger food budget shares at convenience stores and restaurants are linked with poor diet quality based on the healthy eating index-2015 (HEI-2015) scores and higher BMI. We further explored potential heterogeneity on outcomes of interest across income groups. Results suggest heterogeneous effects may exist across income groups: low-income households, who spent a larger share of their food budget at convenience stores and fast-food restaurants are related to poor diet quality and more likely to be obese. Our findings will help improve understanding of the causes of diet-related health problems and may illuminate potential avenues of intervention to address obesity.
Magnesium Influences Membrane Fusion during Myogenesis by Modulating Oxidative Stress in C2C12 Myoblasts
Authors: Zocchi Monica , Béchet Daniel , Mazur André , Maier Jeanette A. , Castiglioni Sara
Published: March 2021

Magnesium (Mg) is essential to skeletal muscle where it plays a key role in myofiber relaxation. Although the importance of Mg in the mature skeletal muscle is well established, little is known about the role of Mg in myogenesis. We studied the effects of low and high extracellular Mg in C2C12 myogenic differentiation. Non-physiological Mg concentrations induce oxidative stress in myoblasts. The increase of reactive oxygen species, which occurs during the early phase of the differentiation process, inhibits myoblast membrane fusion, thus impairing myogenesis. Therefore, correct Mg homeostasis, also maintained through a correct dietary intake, is essential to assure the regenerative capacity of skeletal muscle fibers.
Can Cranberry Juice Protect against Rotenone-Induced Toxicity in Rats?
Authors: Kurpik Monika , Zalewski Przemysław , Kujawska Małgorzata , Ewertowska Małgorzata , Ignatowicz Ewa , Cielecka-Piontek Judyta , Jodynis-Liebert Jadwiga
Published: March 2021

The high polyphenols content of cranberry accounts for its strong antioxidant activity underlying the beneficial health effects of this fruit. Rotenone (ROT) is a specific inhibitor of mitochondrial complex I in the brain which leads to the generation of oxidative stress. To date, there are few data indicating that toxicity of ROT is not limited to the brain but can also affect other tissues. We aimed to examine whether ROT-induced oxidative stress could be counteracted by cranberry juice not only in the brain but also in the liver and kidney. Wistar rats were given the combined treatment with ROT and cranberry juice (CJ) for 35 days. Parameters of antioxidant status were determined in the organs. ROT enhanced lipid peroxidation solely in the brain. The increase in the DNA damage was noticed in all organs examined and in leukocytes. The beneficial effect of CJ on these parameters appeared only in the brain. Additionally, CJ decreased the activity of serum hepatic enzymes. The effect of CJ on antioxidant enzymes was not consistent, however, in some organs, CJ reversed changes evoked by ROT. Summing up, ROT can cause oxidative damage not only in the brain but also in other organs. CJ demonstrated a protective effect against ROT-induced toxicity.
Efficacy of Calcium-Containing Eggshell Powder Supplementation on Urinary Fluoride and Fluorosis Symptoms in Women in the Ethiopian Rift Valley
Authors: Mulualem Demmelash , Hailu Dejene , Tessema Masresha , Whiting Susan J.
Published: March 2021

Dietary calcium binds Fluoride (F), thus preventing excess F absorption. We aimed to assess the efficacy of supplementing calcium-containing Eggshell Powder (ESP) on F absorption using urine F excretion and on fluorosis symptoms. In total, 82 women (41 Intervention Group, IG; 41 Control Group, CG) were recruited; overall, 39 in each group completed the trial. Morning spot urine was collected before (baseline, BL) and after (endline, EL) the intervention that was 6-months daily supplementation with 2.4 g ESP (providing ~1000 mg of calcium). Dental, skeletal, and non-skeletal fluorosis assessments was carried out at BL and, except for dental, at EL. Relative risk (RR) and linear generalized estimating equation were used to compare outcomes between groups. At BL, urinary F excretion in the IG and CG groups was similar, ~10 mg/L. At EL, urinary F excretion in IG women was six-fold lower (β = −6.1 (95% CI: −7.1, −5.1)) compared to CG. The risk of developing skeletal and non-skeletal fluorosis were significantly (p < 0.001) reduced in the intervention group. A significant reduction in urinary F excretion and reduction in many fluorosis symptoms were observed among women supplemented with calcium-containing ESP, thus providing evidence for using this dietary calcium source for mitigation of fluorosis. Clinical trials registration: NCT03355222.
University Students Purchasing Food on Campus More Frequently Consume More Energy-Dense, Nutrient-Poor Foods: A Cross-Sectional Survey
Authors: Whatnall Megan C , Soo Zhao Min , Patterson Amanda J , Hutchesson Melinda J
Published: March 2021

University food environments are typically dominated by unhealthy food choices. The aim was to investigate associations between on-campus food purchasing behaviours and dietary intake in an Australian university student sample. An online cross-sectional survey was conducted in 2017–2018 with students (n = 362, 71.0% female, mean age 27.5 ± 10.5 years) from the University of Newcastle, Australia. On-campus food purchasing behaviours (purchasing frequency and weekly expenditure), dietary intake (diet quality and percentage energy/day from energy-dense, nutrient-poor (EDNP) foods) and sociodemographic and student characteristics (e.g., time spent on campus) were measured. Linear regression was used to explore associations between food purchasing behaviours and dietary intake, adjusted for potential confounders. Mean percentage energy/day from EDNP foods was 31.7 ± 14.4. Mean diet quality score was 32.6 ± 10.2 out of 73. Higher percentage energy/day from EDNP foods was associated with higher weekly expenditure (β = 0.203, p < 0.001) and more frequent purchase (β = 18.041, p < 0.001 for ≥4 times a week vs. never) of food/drinks on campus. Diet quality was not significantly associated with purchase frequency or expenditure (p > 0.05). Findings are supportive of changes being made to university food environments, as a strategy to improve dietary intake among university students.
Iron Deficiency without Anemia Decreases Physical Endurance and Mitochondrial Complex I Activity of Oxidative Skeletal Muscle in the Mouse
Authors: Rineau Emmanuel , Gueguen Naïg , Procaccio Vincent , Geneviève Franck , Reynier Pascal , Henrion Daniel , Lasocki Sigismond
Published: March 2021

Iron deficiency (ID), with or without anemia, is responsible for physical fatigue. This effect may be linked to an alteration of mitochondrial metabolism. Our aim was to assess the impact of ID on skeletal striated muscle mitochondrial metabolism. Iron-deficient non-anemic mice, obtained using a bloodletting followed by a low-iron diet for three weeks, were compared to control mice. Endurance was assessed using a one-hour submaximal exercise on a Rotarod device and activities of mitochondrial complexes I and IV were measured by spectrophotometry on two types of skeletal striated muscles, the soleus and the quadriceps. As expected, ID mice displayed hematologic markers of ID and reduced iron stores, although none of them were anemic. In ID mice, endurance was significantly reduced and activity of the respiratory chain complex I, normalized to citrate synthase activity, was significantly reduced in the soleus muscle but not in the quadriceps. Complex IV activities were not significantly different, neither in the soleus nor in the quadriceps. We conclude that ID without anemia is responsible for impaired mitochondrial complex I activity in skeletal muscles with predominant oxidative metabolism. These results bring pathophysiological support to explain the improved physical activity observed when correcting ID in human. Further studies are needed to explore the mechanisms underlying this decrease in complex I activity and to assess the role of iron therapy on muscle mitochondrial metabolism.
Resting Energy Expenditure Is Elevated in Asthma
Authors: Mey Jacob T. , Matuska Brittany , Peterson Laura , Wyszynski Patrick , Koo Michelle , Sharp Jacqueline , Pennington Emily , McCarroll Stephanie , Micklewright Sarah , Zhang Peng , Aronica Mark , Hoddy Kristin K. , Champagne Catherine M. , Heymsfield Steven B. , Comhair Suzy A. A. , Kirwan John P. , Erzurum Serpil C. , Mulya Anny
Published: March 2021

Background: Asthma physiology affects respiratory function and inflammation, factors that may contribute to elevated resting energy expenditure (REE) and altered body composition. Objective: We hypothesized that asthma would present with elevated REE compared to weight-matched healthy controls. Methods: Adults with asthma (n = 41) and healthy controls (n = 20) underwent indirect calorimetry to measure REE, dual-energy X-ray absorptiometry (DEXA) to measure body composition, and 3-day diet records. Clinical assessments included spirometry, fractional exhaled nitric oxide (FENO), and a complete blood count. Results: Asthmatics had greater REE than controls amounting to an increase of ~100 kcals/day, even though body mass index (BMI) and body composition were similar between groups. Inclusion of asthma status and FENO in validated REE prediction equations led to improved estimates. Further, asthmatics had higher white blood cell (control vs. asthma (mean ± SD): 4.7 ± 1.1 vs. 5.9 ± 1.6, p < 0.01) and neutrophil (2.8 ± 0.9 vs. 3.6 ± 1.4, p = 0.02) counts that correlated with REE (both p < 0.01). Interestingly, despite higher REE, asthmatics reported consuming fewer calories (25.1 ± 7.5 vs. 20.3 ± 6.0 kcals/kg/day, p < 0.01) and carbohydrates than controls. Conclusion: REE is elevated in adults with mild asthma, suggesting there is an association between REE and the pathophysiology of asthma.
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study
Authors: Salamon Dominika , Sroka-Oleksiak Agnieszka , Gurgul Artur , Arent Zbigniew , Szopa Magdalena , Bulanda Małgorzata , Małecki Maciej T. , Gosiewski Tomasz
Published: March 2021

The studies on microbiome in the human digestive tract indicate that fungi could also be one of the external factors affecting development of diabetes. The aim of this study was to evaluate the quantitative and qualitative mycobiome composition in the colon of the adults with type 1 (T1D), n = 26 and type 2 (T2D) diabetes, n = 24 compared to the control group, n = 26. The gut mycobiome was characterized in the stool samples using the analysis of the whole internal transcribed spacer (ITS) region of the fungal rDNA gene cluster by next-generation sequencing (NGS) with increased sensitivity. At the L2 (phylum) level, Basidiomycota fungi were predominant in all 3 study groups. Group T1D presented significantly lower number of Ascomycota compared to the T2D group, and at the L6 (genus) level, the T1D group presented significantly lower number of Saccharomyces genus compared to control and T2D groups. In the T1D group, a significant positive correlation between total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and fungi of the genus Saccharomyces, and in the T2D group, a negative correlation between the total cholesterol level and Malassezia genus was found. The obtained results seem to be a good foundation to extend the analysis of the relationship between individual genera and species of fungi and the parameters determining the metabolism of carbohydrates and lipids in the human body.
Food and Food Groups in Inflammatory Bowel Disease (IBD): The Design of the Groningen Anti-Inflammatory Diet (GrAID)
Authors: Campmans-Kuijpers Marjo J. E. , Dijkstra Gerard
Published: March 2021

Diet plays a pivotal role in the onset and course of inflammatory bowel disease (IBD). Patients are keen to know what to eat to reduce symptoms and flares, but dietary guidelines are lacking. To advice patients, an overview of the current evidence on food (group) level is needed. This narrative review studies the effects of food (groups) on the onset and course of IBD and if not available the effects in healthy subjects or animal and in vitro IBD models. Based on this evidence the Groningen anti-inflammatory diet (GrAID) was designed and compared on food (group) level to other existing IBD diets. Although on several foods conflicting results were found, this review provides patients a good overview. Based on this evidence, the GrAID consists of lean meat, eggs, fish, plain dairy (such as milk, yoghurt, kefir and hard cheeses), fruit, vegetables, legumes, wheat, coffee, tea and honey. Red meat, other dairy products and sugar should be limited. Canned and processed foods, alcohol and sweetened beverages should be avoided. This comprehensive review focuses on anti-inflammatory properties of foods providing IBD patients with the best evidence on which foods they should eat or avoid to reduce flares. This was used to design the GrAID.
Effects of n-3 Polyunsaturated Fatty Acid Supplementation in the Prevention and Treatment of Depressive Disorders—A Systematic Review and Meta-Analysis
Authors: Wolters Maike , von der Haar Annkathrin , Baalmann Ann-Kristin , Wellbrock Maike , Heise Thomas L. , Rach Stefan
Published: March 2021

N-3 polyunsaturated fatty acids (PUFAs) have been suggested to affect depressive disorders. This review aims to determine the effect of n-3 PUFAs on depressive symptoms in people with or without diagnosed depression. Medline, PsycINFO, and Cochrane CENTRAL databases were searched for randomized controlled trials (RCTs) assessing the association between n-3 PUFAs and depressive symptoms or disorders as outcomes. A random-effects meta-analysis of standardized mean difference (SMD) with 95% confidence intervals (CI) was performed. Twenty-five studies (7682 participants) were included. Our meta-analysis (20 studies) indicated that n-3 PUFA supplementation lowered depressive symptomology as compared with placebo: SMD = −0.34, 95% CI: −0.55, −0.12, I2 = 86%, n = 5836, but a possible publication bias cannot be ruled out. Subgroup analyses indicated no statistically significant difference by treatment duration of <12 vs. ≥12 weeks, presence of comorbidity, or severity of depressive symptoms. Nevertheless, beneficial effects were seen in the subgroups of studies with longer treatment duration and with no depression and mild to moderate depression. Subgroup analysis by eicosapentaenoic acid (EPA) dosage revealed differences in favor of the lower EPA dosage. Sensitivity analysis including studies with low risk of bias seems to confirm the overall result. Supplementation of n-3 PUFA appears to have a modest beneficial effect on depressive symptomology, although the quality of evidence is still insufficient.