bluuebunny/crossref_metadata_2025 huggingface dataset:
Physalis alkekengi L. Calyx Extract Alleviates Glycolipid Metabolic Disturbance and Inflammation by Modulating Gut Microbiota, Fecal Metabolites, and Glycolipid Metabolism Gene Expression in Obese Mice
Authors: Li Lin , Wang Xiaolong , Zhou Ying , Yan Na , Gao Han , Sun Xiaojie , Zhang Chunjing
Published: May 2023

Physalis alkekengi L. calyx (PC) extract can relieve insulin resistance and has glycemic and anti-inflammatory effects; however, the potential mechanisms related to gut microbiota and metabolites remain elusive. This study aimed to understand how PC regulates gut microbiota and metabolites to exert anti-obesogenic effects and relieve insulin resistance. In this study, a high-fat high-fructose (HFHF)-diet-induced obesity C57BL/6J male mice model with glycolipid metabolism dysfunction was established, which was supplemented with the aqueous extract of PC daily for 10 weeks. The results showed that the PC supplementation could effectively cure the abnormal lipid metabolism and maintain glucose metabolism homeostasis by regulating the expression of adipose metabolic genes and glucose metabolism genes in the liver, thereby effectively alleviating the inflammatory response. PC treatment also increased the contents of fecal short-chain fatty acids (SCFAs), especially butyric acid. PC extract could restore the HFHF-disrupted diversity of gut microbiota by significantly increasing the relative abundance of Lactobacillus and decreasing those of Romboutsia, Candidatus_Saccharimonas, and Clostridium_sensu_stricto_1. The negative effects of the HFHF diet were ameliorated by PC by regulating multiple metabolic pathways, such as lipid metabolism (linoleic acid metabolism, alpha-linolenic acid metabolism, and sphingolipid metabolism) and amino acid metabolism (histidine and tryptophan metabolism). Correlation analysis showed that among the obesity parameters, gut microbiota and metabolites are directly and closely related. To sum up, this study suggested that PC treatment exhibited therapeutic effects by regulating the gut microbiota, fecal metabolites, and gene expression in the liver to improve glucose metabolism, modulate adiposity, and reduce inflammation.
Validity and Reliability of the Korean Version of the Weight Self-Stigma Questionnaire (WSSQ-K)
Authors: Park Seyoen , Seo Kawoun
Published: May 2023

Weight self-stigma refers to negative self-prejudice due to the internalization of negative social messages about one’s weight. People with high self-stigma may have low self-esteem and decreased social activity. Weight-related self-stigma can create diet-related disorders since it is highly related to the recognition of body types. However, there are no tools available to measure the weight-related stigma of the general public in Korea. This study evaluated the validity and reliability of the Korean version of the Weight Self-Stigma Questionnaire (WSSQ-K). A methodological study was conducted with 150 Korean university students. Construct validity was evaluated using exploratory factor analysis. The WSSQ-K was correlated with body mass index and measures of self-esteem and weight concern to evaluate concurrent validity. Internal consistency reliability was evaluated with Cronbach’s alpha. In the exploratory factor analysis, two factors were proposed: “self-devaluation” (Cronbach’s α = 79) and “fear of enacted stigma” (Cronbach’s α = 82). Factor loadings for the 12 items on two factors ranged from 0.539 to 0.811, which explained 53.3% of the total variance. The WSSQ-K correlated with body mass index, self-esteem, and weight concern. The findings showed that the WSSQ-K was a reliable and valid measure that could be used for evaluating weight self-stigma in normal-weight adults in Korea.
Unveiling the Relevance of the Oral Cavity as a Staphylococcus aureus Colonization Site and Potential Source of Antimicrobial Resistance
Authors: Campos Joana , Pires Mariana Faria , Sousa Marta , Campos Carla , da Costa Carolina Fernandes Ferreira Alves , Sampaio-Maia Benedita
Published: May 2023

Staphylococcus aureus is both a human commensal and a pathogen, that causes serious nosocomial and community-acquired infections. Despite nostrils being considered its preferred host habitat, the oral cavity has been demonstrated to be an ideal starting point for auto-infection and transmission. The antibiotic resistance assessment of S. aureus is a priority and is often reported in clinical settings. This study aimed to explore the prevalence and antimicrobial susceptibility of S. aureus in the oral and nasal cavities of healthy individuals. The participants (n = 101) were subjected to a demographic and clinical background survey, a caries evaluation, and to oral and nasal swabbing. Swabs were cultured in differential/selective media and S. aureus isolates were identified (MALDI-TOF MS) and tested for antibiotic susceptibility (EUCAST/CLSI). Similar S. aureus prevalence was found exclusively on nasal (13.9%) or oral (12.0%) habitats, whereas 9.9% of the population were simultaneous nasal and oral carriers. In oro-nasal cavities, similar antibiotic resistance rates (83.3–81.5%), including MDR (20.8–29.6%), were observed. Notably, 60% (6/10) of the simultaneous nasal and oral carriers exhibited different antibiotic resistance profiles between cavities. This study demonstrates the relevance of the oral cavity as an independent colonization site for S. aureus and as a potential source of antimicrobial resistance, a role which has been widely neglected so far.
Intracellular Diversity of WNV within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns of Polyinfection
Authors: Talmi-Frank Dalit , Byas Alex D. , Murrieta Reyes , Weger-Lucarelli James , Rückert Claudia , Gallichotte Emily N. , Yoshimoto Janna A. , Allen Chris , Bosco-Lauth Angela M. , Graham Barbara , Felix Todd A. , Brault Aaron C. , Ebel Gregory D.
Published: May 2023

Arthropod-borne virus (arbovirus) populations exist as mutant swarms that are maintained between arthropods and vertebrates. West Nile virus (WNV) population dynamics are host-dependent. In American crows, purifying selection is weak and population diversity is high compared to American robins, which have 100- to 1000-fold lower viremia. WNV passed in robins leads to fitness gains, whereas that passed in crows does not. Therefore, we tested the hypothesis that high crow viremia allows for higher genetic diversity within individual avian peripheral blood mononuclear cells (PBMCs), reasoning that this could have produced the previously observed host-specific differences in genetic diversity and fitness. Specifically, we infected cells and birds with a molecularly barcoded WNV and sequenced viral RNA from single cells to quantify the number of WNV barcodes in each. Our results demonstrate that the richness of WNV populations within crows far exceeds that in robins. Similarly, rare WNV variants were maintained by crows more frequently than by robins. Our results suggest that increased viremia in crows relative to robins leads to the maintenance of defective genomes and less prevalent variants, presumably through complementation. Our findings further suggest that weaker purifying selection in highly susceptible crows is attributable to this higher viremia, polyinfections and complementation.
Current Status and Challenges of Oncolytic Virotherapy for the Treatment of Glioblastoma
Authors: Webb Mason J. , Sener Ugur , Vile Richard G.
Published: May 2023

Despite decades of research and numerous clinical trials, the prognosis of patients diagnosed with glioblastoma (GBM) remains dire with median observed survival at 8 months. There is a critical need for novel treatments for GBM, which is the most common malignant primary brain tumor. Major advances in cancer therapeutics such as immune checkpoint inhibitors and chimeric antigen receptor (CAR) T-cell therapy have not yet led to improved outcomes for GBM. Conventional therapy of surgery followed by chemoradiation with or without tumor treating fields remains the standard of care. One of the many approaches to GBM therapy currently being explored is viral therapies. These typically work by selectively lysing target neoplastic cells, called oncolysis, or by the targeted delivery of a therapeutic transgene via a viral vector. In this review, we discuss the underlying mechanisms of action and describe both recent and current human clinical trials using these viruses with an emphasis on promising viral therapeutics that may ultimately break the field’s current stagnant paradigm.
Design and Optimization of Omeprazole-Curcumin-Loaded Hydrogel Beads Coated with Chitosan for Treating Peptic Ulcers
Authors: Heikal Eman J. , Kaoud Rashad M. , Gad Shadeed , Mokhtar Hatem I. , Aldahish Afaf A. , Alzlaiq Wafa Ali , Zaitone Sawsan A. , Moustafa Yasser M. , Hammady Taha M.
Published: May 2023

This study aimed to formulate a pharmaceutical dosage form containing omeprazole (OMP) and curcumin (CURC) to treat experimental peptic ulcers. OMP and CURC were preliminarily complexed with hydroxypropyl-β-cyclodextrin for enhancing their solubilization. After that, the combined complex (CURC/OMP) was loaded to alginate beads to sustain their release and then coated with chitosan. Finally, we tested the anti-ulcerogenic impact of the best formula versus free OMP or OMP-only-loaded beads. The formulated spherical beads’ diameter ranged from a minimum value of 1.5 ± 0.08 mm to 2.6 ± 0.24 mm; the swelling results ranged from 400.00 ± 8.5% to 800.00 ± 6.2%. The entrapment efficiency was in a range from 60.85 ± 1.01% to 87.44 ± 1.88%. The optimized formula (F8) showed a maximum EE% (87.44 ± 1.88%), swelling (800.00 ± 6.2%), and diameter in the range of 2.60 ± 0.24, with a desirability of 0.941. In the first hour following the administration of the free drug complex, 95% of OMP and 98% of CURC were released. This is unacceptable for medications that require a delayed release in the stomach. The initial drug release from hydrogel beads was 23.19% for CURC and 17.19% for OMP after 2 h and 73.09% for CURC and 58.26% for OMP after 12 h; however, after 24 h, 87.81% of CURC and 81.67% of OMP had been released. The OMP/CURC beads showed a more stable particle size (0.52 ± 0.01 mm) after 6 weeks. In conclusion, the OMP/CURC hydrogel beads give stronger anti-ulcer effectiveness compared to free OMP, CURC-only beads, and OMP-only-loaded beads, indicating a prospective application for managing peptic ulcers.
Measurement of Patient-Derived Glioblastoma Cell Response to Temozolomide Using Fluorescence Lifetime Imaging of NAD(P)H
Authors: Yuzhakova Diana V. , Sachkova Daria A. , Shirmanova Marina V. , Mozherov Artem M. , Izosimova Anna V. , Zolotova Anna S. , Yashin Konstantin S.
Published: May 2023

Personalized strategies in glioblastoma treatment are highly necessary. One of the possible approaches is drug screening using patient-derived tumor cells. However, this requires reliable methods for assessment of the response of tumor cells to treatment. Fluorescence lifetime imaging microscopy (FLIM) is a promising instrument to detect early cellular response to chemotherapy using the autofluorescence of metabolic cofactors. Here, we explored FLIM of NAD(P)H to evaluate the sensitivity of patient-derived glioma cells to temozolomide (TMZ) in vitro. Our results demonstrate that the more-responsive cell cultures displayed the longest mean fluorescence lifetime τm after TMZ treatment due to an increase in the protein-bound NAD(P)H fraction α2 associated with a shift to oxidative phosphorylation. The cell cultures that responded poorly to TMZ had generally shorter τm, i.e., were more glycolytic, and showed no or insignificant changes after treatment. The FLIM data correlate well with standard measurements of cellular drug response—cell viability and proliferation index and clinical response in patients. Therefore, FLIM of NAD(P)H provides a highly sensitive, label-free assay of treatment response directly on patient-derived glioblastoma cells and can become an innovative platform for individual drug screening for patients.
Synthesis and Preclinical Evaluation of Novel 68Ga-Labeled (R)-Pyrrolidin-2-yl-boronic Acid-Based PET Tracers for Fibroblast Activation Protein-Targeted Cancer Imaging
Authors: Bendre Shreya , Kuo Hsiou-Ting , Merkens Helen , Zhang Zhengxing , Wong Antonio A. W. L. , Bénard François , Lin Kuo-Shyan
Published: May 2023

Fibroblast activation protein (FAP) is a membrane-tethered serine protease overexpressed in the reactive stromal fibroblasts of >90% human carcinomas, which makes it a promising target for developing radiopharmaceuticals for the imaging and therapy of carcinomas. Here, we synthesized two novel (R)-pyrrolidin-2-yl-boronic acid-based FAP-targeted ligands: SB02055 (DOTA-conjugated (R)-(1-((6-(3-(piperazin-1-yl)propoxy)quinoline-4-carbonyl)glycyl)pyrrolidin-2-yl)boronic acid) and SB04028 (DOTA-conjugated ((R)-1-((6-(3-(piperazin-1-yl)propoxy)quinoline-4-carbonyl)-D-alanyl)pyrrolidin-2-yl)boronic acid). natGa- and 68Ga-complexes of both ligands were evaluated in preclinical studies and compared to previously reported natGa/68Ga-complexed PNT6555. Enzymatic assays showed that FAP binding affinities (IC50) of natGa-SB02055, natGa-SB04028 and natGa-PNT6555 were 0.41 ± 0.06, 13.9 ± 1.29 and 78.1 ± 4.59 nM, respectively. PET imaging and biodistribution studies in HEK293T:hFAP tumor-bearing mice showed that while [68Ga]Ga-SB02055 presented with a nominal tumor uptake (1.08 ± 0.37 %ID/g), [68Ga]Ga-SB04028 demonstrated clear tumor visualization with ~1.5-fold higher tumor uptake (10.1 ± 0.42 %ID/g) compared to [68Ga]Ga-PNT6555 (6.38 ± 0.45 %ID/g). High accumulation in the bladder indicated renal excretion of all three tracers. [68Ga]Ga-SB04028 displayed a low background level uptake in most normal organs, and comparable to [68Ga]Ga-PNT6555. However, since its tumor uptake was considerably higher than [68Ga]Ga-PNT6555, the corresponding tumor-to-organ uptake ratios for [68Ga]Ga-SB04028 were also significantly greater than [68Ga]Ga-PNT6555. Our data demonstrate that (R)-(((quinoline-4-carbonyl)-d-alanyl)pyrrolidin-2-yl)boronic acid is a promising pharmacophore for the design of FAP-targeted radiopharmaceuticals for cancer imaging and radioligand therapy.
Eudragit® FS Microparticles Containing Bacteriophages, Prepared by Spray-Drying for Oral Administration
Authors: Tabare Emilie , Dauchot Tiffany , Cochez Christel , Glonti Tea , Antoine Céline , Laforêt Fanny , Pirnay Jean-Paul , Delcenserie Véronique , Thiry Damien , Goole Jonathan
Published: May 2023

Phage therapy is recognized to be a promising alternative to fight antibiotic-resistant infections. In the quest for oral dosage forms containing bacteriophages, the utilization of colonic-release Eudragit® derivatives has shown potential in shielding bacteriophages from the challenges encountered within the gastrointestinal tract, such as fluctuating pH levels and the presence of digestive enzymes. Consequently, this study aimed to develop targeted oral delivery systems for bacteriophages, specifically focusing on colon delivery and employing Eudragit® FS30D as the excipient. The bacteriophage model used was LUZ19. An optimized formulation was established to not only preserve the activity of LUZ19 during the manufacturing process but also ensure its protection from highly acidic conditions. Flowability assessments were conducted for both capsule filling and tableting processes. Furthermore, the viability of the bacteriophages remained unaffected by the tableting process. Additionally, the release of LUZ19 from the developed system was evaluated using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Finally, stability studies demonstrated that the powder remained stable for at least 6 months when stored at +5 °C.
Solid Lipid Nanoparticles Containing Morin: Preparation, Characterization, and Ex Vivo Permeation Studies
Authors: De Gaetano Federica , Celesti Consuelo , Paladini Giuseppe , Venuti Valentina , Cristiano Maria Chiara , Paolino Donatella , Iannazzo Daniela , Strano Vincenza , Gueli Anna M. , Tommasini Silvana , Ventura Cinzia Anna , Stancanelli Rosanna
Published: May 2023

In recent years, bioactive compounds have been the focus of much interest in scientific research, due to their low toxicity and extraordinary properties. However, they possess poor solubility, low chemical stability, and unsustainable bioavailability. New drug delivery systems, and among them solid lipid nanoparticles (SLNs), could minimize these drawbacks. In this work, morin (MRN)-loaded SLNs (MRN-SLNs) were prepared using a solvent emulsification/diffusion method, using two different lipids, Compritol® 888 ATO (COM) or Phospholipon® 80H (PHO). SLNs were investigated for their physical–chemical, morphological, and technological (encapsulation parameters and in vitro release) properties. We obtained spherical and non-aggregated nanoparticles with hydrodynamic radii ranging from 60 to 70 nm and negative zeta potentials (about −30 mV and −22 mV for MRN-SLNs-COM and MRN-SLNs-PHO, respectively). The interaction of MRN with the lipids was demonstrated via μ-Raman spectroscopy, X-ray diffraction, and DSC analysis. High encapsulation efficiency was obtained for all formulations (about 99%, w/w), particularly for the SLNs prepared starting from a 10% (w/w) theoretical MRN amount. In vitro release studies showed that about 60% of MRN was released within 24 h and there was a subsequent sustained release within 10 days. Finally, ex vivo permeation studies with excised bovine nasal mucosa demonstrated the ability of SLNs to act as a penetration enhancer for MRN due to the intimate contact and interaction of the carrier with the mucosa.
Antimicrobial Biomaterials for Chronic Wound Care
Authors: Miron Adrian , Giurcaneanu Calin , Mihai Mara Madalina , Beiu Cristina , Voiculescu Vlad Mihai , Popescu Marius Nicolae , Soare Elena , Popa Liliana Gabriela
Published: May 2023

Chronic wounds encompass a myriad of lesions, including venous and arterial leg ulcers, diabetic foot ulcers (DFUs), pressure ulcers, non-healing surgical wounds and others. Despite the etiological differences, chronic wounds share several features at a molecular level. The wound bed is a convenient environment for microbial adherence, colonization and infection, with the initiation of a complex host–microbiome interplay. Chronic wound infections with mono- or poly-microbial biofilms are frequent and their management is challenging due to tolerance and resistance to antimicrobial therapy (systemic antibiotic or antifungal therapy or antiseptic topicals) and to the host’s immune defense mechanisms. The ideal dressing should maintain moisture, allow water and gas permeability, absorb wound exudates, protect against bacteria and other infectious agents, be biocompatible, be non-allergenic, be non-toxic and biodegradable, be easy to use and remove and, last but not least, it should be cost-efficient. Although many wound dressings possess intrinsic antimicrobial properties acting as a barrier to pathogen invasion, adding anti-infectious targeted agents to the wound dressing may increase their efficiency. Antimicrobial biomaterials may represent a potential substitute for systemic treatment of chronic wound infections. In this review, we aim to describe the available types of antimicrobial biomaterials for chronic wound care and discuss the host response and the spectrum of pathophysiologic changes resulting from the contact between biomaterials and host tissues.
A Review on Image Sensor Communication and Its Applications to Vehicles
Authors: Huang Ruiyi , Yamazato Takaya
Published: May 2023

Image sensor communication (ISC), also known as optical camera communication, is a form of visible light communication that utilizes image sensors rather than a single photodiode, for data reception. ISC offers spatial separation properties and robustness to ambient noise, making it suitable for outdoor applications such as intelligent transportation systems (ITSs). This review analyzes the research trends in ISC, specifically concerning its application in ITSs. Our focus is on various ISC receivers, including rolling shutter cameras, global shutter high-speed cameras, optical communication image sensors, and event cameras. We analyze how each of these receivers is being utilized in ISC vehicular applications. In addition, we highlight the use of ISC in range estimation techniques and the ability to achieve simultaneous communication and range estimation. By examining these topics, we aim to provide a comprehensive overview of the role of ISC technology in ITSs and its potential for future development.
High–Power 792 nm Fiber–Coupled Semiconductor Laser
Authors: Liu Peng , Sun Wanggen , Sun Xiao , Zhu Zhen , Qin Huabing , Su Jian , Liu Chengcheng , Tang Wenjing , Jiang Kai , Xia Wei , Xu Xiangang
Published: May 2023

The pumping of Tm-doped crystal or fiber by a 792 nm semiconductor laser is an important way to generate a mid-infrared laser, which is widely used in various fields. In this paper, a high–power 792 nm fiber–coupled semiconductor laser module was successfully fabricated with the output power of 232 W at a 10 A continuous current and the electro-optic conversion efficiency of 48.6%. The laser module is coupled with 24 chips into a fiber by spatial multiplexing and polarization combination technology. For a single emitting laser chip, the continuous wave (CW) output power and threshold current are 10.45 W at 10 A and 1.55 A, respectively. A polarization as high as 94% can also be realized, which is more suitable for laser spatial beam combining. The laser module was aged for more than 4000 h at 12 A and 25 °C without obvious power degradation.
Seven-Grooved-Rod, Side-Pumping Concept for Highly Efficient TEM00-Mode Solar Laser Emission through Fresnel Lenses
Authors: Costa Hugo , Liang Dawei , Almeida Joana , Catela Miguel , Garcia Dário , Tibúrcio Bruno D. , Vistas Cláudia R.
Published: May 2023

Low-cost, lightweight, and easily available Fresnel lenses are a more alluring choice for solar laser power production, when compared to the costly and complex heliostat-parabolic mirror systems. Therefore, a seven-rod solar laser head was designed and numerically studied to enhance the efficiency in TEM00-mode laser power production, employing six Fresnel lenses with 10 m2 total collection area for collection and concentration of sunlight. Six folding mirrors redirected the solar rays towards the laser head, composed of six fused silica aspheric lenses and rectangular compound parabolic concentrators paired together for further concentration, and a cylindrical cavity, in which seven Nd:YAG rods were mounted and side-pumped. With conventional rods, total TEM00-mode laser power reached 139.89 W, which is equivalent to 13.99 W/m2 collection efficiency and 1.47% solar-to-TEM00-mode laser power conversion efficiency. More importantly, by implementing rods with grooved sidewalls, the total laser power was increased to 153.29 W, corresponding to 15.33 W/m2 collection and 1.61% conversion efficiencies. The side-pumping configuration and the good thermal performance may ensure that the seven-grooved-rod system has better scalability than other previously proposed schemes.
Barley Cultivar Sarab 1 Has a Characteristic Region on the Thylakoid Membrane That Protects Photosystem I under Iron-Deficient Conditions
Authors: Saito Akihiro , Hoshi Kimika , Wakabayashi Yuna , Togashi Takumi , Shigematsu Tomoki , Katori Maya , Ohyama Takuji , Higuchi Kyoko
Published: May 2023

The barley cultivar Sarab 1 (SRB1) can continue photosynthesis despite its low Fe acquisition potential via roots and dramatically reduced amounts of photosystem I (PSI) reaction-center proteins under Fe-deficient conditions. We compared the characteristics of photosynthetic electron transfer (ET), thylakoid ultrastructure, and Fe and protein distribution on thylakoid membranes among barley cultivars. The Fe-deficient SRB1 had a large proportion of functional PSI proteins by avoiding P700 over-reduction. An analysis of the thylakoid ultrastructure clarified that SRB1 had a larger proportion of non-appressed thylakoid membranes than those in another Fe-tolerant cultivar, Ehimehadaka-1 (EHM1). Separating thylakoids by differential centrifugation further revealed that the Fe-deficient SRB1 had increased amounts of low/light-density thylakoids with increased Fe and light-harvesting complex II (LHCII) than did EHM1. LHCII with uncommon localization probably prevents excessive ET from PSII leading to elevated NPQ and lower PSI photodamage in SRB1 than in EHM1, as supported by increased Y(NPQ) and Y(ND) in the Fe-deficient SRB1. Unlike this strategy, EHM1 may preferentially supply Fe cofactors to PSI, thereby exploiting more surplus reaction center proteins than SRB1 under Fe-deficient conditions. In summary, SRB1 and EHM1 support PSI through different mechanisms during Fe deficiency, suggesting that barley species have multiple strategies for acclimating photosynthetic apparatus to Fe deficiency.
Factors Affecting the Germination Ecology of Pimelea trichostachya and Its Relationship to Field Emergence
Authors: Saleem Rashid , Campbell Shane , Fletcher Mary T. , Kalaipandian Sundaravelpandian , Adkins Steve
Published: May 2023

Pimelea trichostachya Lindl is a little-understood Australian native plant, with irregular field emergence, causing significant poisoning to grazing livestock. The study aims to examine the form of dormancy exhibited by P. trichostachya and determine how key environmental conditions, such as alternating temperature and light conditions, moisture availability, substrate pH and burial depth, affect its germination and emergence. The study concludes that P. trichostachya has a complex dormancy mechanism. This comprises a physical component that can be partly removed by fruit scarification, a metabolic dormancy that can be overcome by gibberellic acid (GA3), and a suspected third mechanism based on a water-soluble germination inhibitor. The results showed that scarified single seeded fruit (hereafter seed) with GA3 treatment gave the highest germination percentage (86 ± 3%) at 25/15 °C, with good germination rates at other temperature regimes. Light exposure stimulated germination, but a significant proportion of seeds still germinated in the dark. The study also found that seeds could germinate under water-limited conditions and a wide range of pH levels (4 to 8). Seedling emergence was inhibited when seeds were buried below 3 cm in soil. Pimelea trichostachya emergence in the field commonly occurs from Autumn to Spring. Understanding its dormancy mechanism and recognizing its triggers for germination will enable better prediction of outbreaks. This can help landholders prepare for emergence and help manage seedbank build-up in pastures and crops.
Transcriptional and Post-Translational Regulation of Plant bHLH Transcription Factors during the Response to Environmental Stresses
Authors: Radani Yasmina , Li Rongxue , Korboe Harriet Mateko , Ma Hongyu , Yang Liming
Published: May 2023

Over the past decades, extensive research has been conducted to identify and characterize various plant transcription factors involved in abiotic stress responses. Therefore, numerous efforts have been made to improve plant stress tolerance by engineering these transcription factor genes. The plant basic Helix–Loop–Helix (bHLH) transcription factor family represents one of the most prominent gene families and contains a bHLH motif that is highly conserved in eukaryotic organisms. By binding to specific positions in promoters, they activate or repress the transcription of specific response genes and thus affect multiple variables in plant physiology such as the response to abiotic stresses, which include drought, climatic variations, mineral deficiencies, excessive salinity, and water stress. The regulation of bHLH transcription factors is crucial to better control their activity. On the one hand, they are regulated at the transcriptional level by other upstream components; on the other hand, they undergo various modifications such as ubiquitination, phosphorylation, and glycosylation at the post-translational level. Modified bHLH transcription factors can form a complex regulatory network to regulate the expression of stress response genes and thus determine the activation of physiological and metabolic reactions. This review article focuses on the structural characteristics, classification, function, and regulatory mechanism of bHLH transcription factor expression at the transcriptional and post-translational levels during their responses to various abiotic stress conditions.
In Vitro Propagation of Origanum scabrum (Boiss. & Heldr.): An Endemic Medicinal Plant of Greece
Authors: Alexopoulos Alexios A. , Kartsonas Epaminondas , Karras Stavros , Mavrommati Eleni , Petropoulos Spyridon A. , Papafotiou Maria
Published: May 2023

The aim of the study was to develop an efficient micropropagation protocol of Origanum scabrum, which will allow its commercial exploitation in the pharmaceutical and horticultural industries. First, the effect of the date of the explant collection (20 April, 20 May, 20 June, 20 July, 20 August) and the position of the explant on the plant stem (shoot apex, 1st node, 3rd node, 5th node) on the establishment of in vitro cultures was studied (1st experiment: Stage I). Next, the effect of temperature (15 °C, 25 °C) and the node position (microshoot apex, 1st node, 5th node) on the microplant production and ex vitro survival of plantlets was studied (2nd experiment: Stage II). The optimum season to collect explants from wild plants was shown to be during the vegetative growth of the plants (April to May), while the shoot apex and the 1st node were the most suitable explants. For the proliferation and production of rooted microplants, the best results were obtained from single-node explants excised from microshoots produced from 1st node-explants collected on 20th of May. Temperature did not affect microshoot number, leaf number and the percentage of rooted microplants, while microshoot length was higher at 25 °C. Moreover, microshoot length and the percentage of rooted microplants were higher in those derived from apex explants, while the survival of plantlets was not affected by treatments and ranged between 67% and 100%.
Components of the Phenylpropanoid Pathway in the Implementation of the Protective Effect of Sodium Nitroprusside on Wheat under Salinity
Authors: Maslennikova Dilara , Ivanov Sergey , Petrova Svetlana , Burkhanova Guzel , Maksimov Igor , Lastochkina Oksana
Published: May 2023

Nitric oxide (NO) is a multifunctional, gaseous signaling molecule implicated in both physiological and protective responses to biotic and abiotic stresses, including salinity. In this work, we studied the effects of 200 µM exogenous sodium nitroprusside (SNP, a donor of NO) on the components of the phenylpropanoid pathway, such as lignin and salicylic acid (SA), and its relationship with wheat seedling growth under normal and salinity (2% NaCl) conditions. It was established that exogenous SNP contributed to the accumulation of endogenous SA and increased the level of transcription of the pathogenesis-related protein 1 (PR1) gene. It was found that endogenous SA played an important role in the growth-stimulating effect of SNP, as evidenced by the growth parameters. In addition, under the influence of SNP, the activation of phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and peroxidase (POD), an increase in the level of transcription of the TaPAL and TaPRX genes, and the acceleration of lignin accumulation in the cell walls of roots were revealed. Such an increase in the barrier properties of the cell walls during the period of preadaptation played an important role in protection against salinity stress. Salinity led to significant SA accumulation and lignin deposition in the roots, strong activation of TAL, PAL, and POD, and suppression of seedling growth. Pretreatment with SNP under salinity conditions resulted in additional lignification of the root cell walls, decreased stress-induced endogenous SA generation, and lower PAL, TAL, and POD activities in comparison to untreated stressed plants. Thus, the obtained data suggested that during pretreatment with SNP, phenylpropanoid metabolism was activated (i.e., lignin and SA), which contributed to reducing the negative effects of salinity stress, as evidenced by the improved plant growth parameters.
Green Synthesis of Zinc Oxide Nanoparticles Using Viscum album Extracts: Unveiling Bioactive Compounds, Antibacterial Potential, and Antioxidant Activities
Authors: Mushtaq Waheeda , Ishtiaq Muhammad , Maqbool Mehwish , Mazhar Muhammad Waqas , Casini Ryan , Abd-ElGawad Ahmed M. , Elansary Hosam O.
Published: May 2023

The current study explored the antioxidant and antibacterial capabilities of zinc oxide nanoparticles (ZnONPs) synthetized using methanolic leaf extracts of the medicinal herb Viscum album. Through TEM investigation and UV–Vis analysis, which peaked at 406 nm, the synthesis of ZnONPs was verified. TEM analyses showed that the synthesized ZnONPs had a size distribution with an average of 13.5 nm and a quasi-spherical shape. Forty-four phytoconstituents were found in the methanolic leaf extracts of V. album. Additionally, a comparison of the antibacterial effectiveness and antioxidant capacity of aqueous and methanolic extracts of wild-grown V. album phytomedicine and green-manufactured ZnONPs was conducted. The green-generated ZnONPs were examined against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and shown to have superior antibacterial activity by 22%, 66%, and 44%, respectively, as compared to wild herbal medicinal extracts. Since the ZnONPs’ aqueous extracts had higher concentrations of DNA gyrase-B inhibitory components, they were shown to be more effective in limiting bacterial growth. In contrast to the percentages of 49% and 57% for a wild plant extract, the aqueous- and methanolic-extract-mediated green ZnONPs, with a 100 g/mL concentration, showed 94% and 98% scavenging capacity for DPPH free radicals, respectively. However, methanolic extracts were more effective than aqueous extracts in terms of the antioxidant analyses. This study establishes that greenly produced ZnONPs have the potential to be used in nanomedicine to treat bacteria that are resistant to a variety of drugs, as well as those with reactive oxygen species toxicity.
Genome-Wide Identification and Characterization of WRKY Transcription Factors and Their Expression Profile in Loropetalum chinense var. rubrum
Authors: Liu Yang , Zhang Yifan , Liu Yang , Lin Ling , Xiong Xingyao , Zhang Donglin , Li Sha , Yu Xiaoying , Li Yanlin
Published: May 2023

The WRKY gene family plays important roles in plant growth and development, as well as in the responses to biotic and abiotic stresses. Loropetalum chinense var. rubrum has high ornamental and medicinal value. However, few WRKY genes have been reported in this plant, and their functions remain unknown. To explore the roles that the WRKY genes play in L. chinense var. rubrum, we identified and characterized 79 LcWRKYs through BLAST homology analysis and renamed them (as LcWRKY1–79) based on their distribution on the chromosomes of L. chinense var. rubrum. In this way, according to their structural characteristics and phylogenetic analysis, they were divided into three groups containing 16 (Group I), 52 (Group II), and 11 (Group III) WRKYs, respectively. LcWRKYs in the same group have similar motifs and gene structures; for instance, Motifs 1, 2, 3, 4, and 10 constitute the WRKY domain and zinc-finger structure. The LcWRKY promoter region contains light response elements (ACE, G-box), stress response elements (TC-rich repeats), hormone response elements (TATC-box, TCA-element), and MYB binding sites (MBS, MBSI). Synteny analysis of LcWRKYs allowed us to establish orthologous relationships among the WRKY gene families of Arabidopsis thaliana, Oryza sativa, Solanum lycopersicum L., Vitis vinifera L., Oryza sativa L., and Zea mays L.; furthermore, analysis of the transcriptomes of mature leaves and flowers from different cultivars demonstrated the cultivar-specific LcWRKY gene expression. The expression levels of certain LcWRKY genes also presented responsive changes from young to mature leaves, based on an analysis of the transcriptome in leaves at different developmental stages. White light treatment led to a significant decrease in the expression of LcWRKY6, 18, 24, 34, 36, 44, 48, 61, 62, and 77 and a significant increase in the expression of LcWRKY41, blue light treatment led to a significant decrease in the expression of LcWRKY18, 34, 50, and 77 and a significant increase in the expression of LcWRKY36 and 48. These results enable a better understanding of LcWRKYs, facilitating the further exploration of their genetic functions and the molecular breeding of L. chinense var. rubrum.
Role of Bovine Serum Albumin Addition in Micellization and Gel Formation of Poloxamer 407
Authors: Hirun Namon , Kraisit Pakorn , Soontaranon Siriwat
Published: May 2023

The combination of the thermoresponsive polymer and protein has demonstrated great promise in its applications in drug delivery and tissue engineering fields. This study described the impact of bovine serum albumin (BSA) on the micellization and sol–gel transition behaviors of poloxamer 407 (PX). The micellization of aqueous PX solutions with and without BSA was examined using isothermal titration calorimetry. In the calorimetric titration curves, the pre-micellar region, the transition concentration region, and the post-micellar region were observed. The presence of BSA had no noticeable impact on critical micellization concentration, but the inclusion of BSA caused the pre-micellar region to expand. In addition to studying the self-organization of PX at a particular temperature, the temperature-induced micellization and gelation of PX were also explored using differential scanning calorimetry and rheology. The incorporation of BSA had no discernible effect on critical micellization temperature (CMT), but it did affect gelation temperature (Tgel) and gel integrity of PX-based systems. The response surface approach illustrated the linear relation between the compositions and the CMT. The major factor affecting the CMT of the mixtures was the concentration of PX. The alteration of the Tgel and the gel integrity were discovered to be a consequence of the intricate interaction between PX and BSA. BSA mitigated the inter-micellar entanglements. Hence, the addition of BSA demonstrated a modulating influence on Tgel and a softening effect on gel integrity. Understanding the influence of serum albumin on the self-assembly and gelation of PX will enable the creation of thermoresponsive drug delivery and tissue engineering systems with controlled gelation temperatures and gel strength.
A Study of Free-Form Shape Rationalization Using Biomimicry as Inspiration
Authors: Dutta Gaurab Sundar , Meiners Dieter , Merkert Nina
Published: May 2023

Bridging the gap between the material and geometrical aspects of a structure is critical in lightweight construction. Throughout the history of structural development, shape rationalization has been of prime focus for designers and architects, with biological forms being a major source of inspiration. In this work, an attempt is made to integrate different phases of design, construction, and fabrication under a single framework of parametric modeling with the help of visual programming. The idea is to offer a novel free-form shape rationalization process that can be realized with unidirectional materials. Taking inspiration from the growth of a plant, we established a relationship between form and force, which can be translated into different shapes using mathematical operators. Different prototypes of generated shapes were constructed using a combination of existing manufacturing processes to test the validity of the concept in both isotropic and anisotropic material domains. Moreover, for each material/manufacturing combination, generated geometrical shapes were compared with other equivalent and more conventional geometrical constructions, with compressive load-test results being the qualitative measure for each use case. Eventually, a 6-axis robot emulator was integrated with the setup, and corresponding adjustments were made such that a true free-form geometry could be visualized in a 3D space, thus closing the loop of digital fabrication.
Hofmeister Series for Conducting Polymers: The Road to Better Electrochemical Activity?
Authors: Volkov Alexey I. , Apraksin Rostislav V.
Published: May 2023

Poly-3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS) is a widely used conducting polymer with versatile applications in organic electronics. The addition of various salts during the preparation of PEDOT:PSS films can significantly influence their electrochemical properties. In this study, we systematically investigated the effects of different salt additives on the electrochemical properties, morphology, and structure of PEDOT:PSS films using a variety of experimental techniques, including cyclic voltammetry, electrochemical impedance spectroscopy, operando conductance measurements and in situ UV-VIS spectroelectrochemistry. Our results showed that the electrochemical properties of the films are closely related to the nature of the additives used and allowed us to establish a probable relationship with the Hofmeister series. The correlation coefficients obtained for the capacitance and Hofmeister series descriptors indicate a strong relationship between the salt additives and the electrochemical activity of PEDOT:PSS films. The work allows us to better understand the processes occurring within PEDOT:PSS films during modification with different salts. It also demonstrates the potential for fine-tuning the properties of PEDOT:PSS films by selecting appropriate salt additives. Our findings can contribute to the development of more efficient and tailored PEDOT:PSS-based devices for a wide range of applications, including supercapacitors, batteries, electrochemical transistors, and sensors.
Solid-State Electrolyte for Lithium-Air Batteries: A Review
Authors: Zhu Qiancheng , Ma Jie , Li Shujian , Mao Deyu
Published: May 2023

Traditional lithium–air batteries (LABs) have been seriously affected by cycle performance and safety issues due to many problems such as the volatility and leakage of liquid organic electrolyte, the generation of interface byproducts, and short circuits caused by the penetration of anode lithium dendrite, which has hindered its commercial application and development. In recent years, the emergence of solid-state electrolytes (SSEs) for LABs well alleviated the above problems. SSEs can prevent moisture, oxygen, and other contaminants from reaching the lithium metal anode, and their inherent performance can solve the generation of lithium dendrites, making them potential candidates for the development of high energy density and safety LABs. This paper mainly reviews the research progress of SSEs for LABs, the challenges and opportunities for synthesis and characterization, and future strategies are addressed.
Enzymatic and Synthetic Routes of Castor Oil Epoxidation
Authors: Montenegro Juliana A. S. , Ries Andreas , Silva Ingridy D. S. , Luna Carlos B. B. , Souza Antônia L. , Wellen Renate M. R.
Published: May 2023

Epoxidation of castor oil in synthetic and enzymatic routes was carried out in order to promote a system with less environmental impact. The epoxidation reactions of castor oil compounds upon addition of lipase enzyme with and without acrylic immobilization and with reaction times of 24 and 6 h, as well as the synthetic compounds upon addition of Amberlite resin and formic acid, were investigated using Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance in hydrogen molecules (1H-NMR). The analysis indicated that the enzymatic reactions (6 h) and synthetic reactions provided a conversion from 50 to 96% and epoxidation from 25 to 48%, resulting from peak stretching and signal disintegration in the hydroxyl region due to the appearance of H2O in the interaction of peracid with catalyst. In systems without toluene, a dehydration event with a peak absorbance of 0.02 AU, indicating a possible vinyl group at 2355 cm−1 in enzymatic reactions without acrylic immobilization, was observed and resulted in a selectivity of 2%. In the absence of a solid catalyst, an unsaturation conversion of castor oil above 90% was achieved; however, this catalyst is necessary for the epoxidation to take place, whereas the lipase enzyme becomes able of epoxidizing and dehydrating the castor oil upon changing the time or reaction system. The conversation from 28 to 48% of solid catalysts (Amberlite and lipase enzyme) displays their importance to the instauration conversion of castor oil into oxirane rings.
Layer-by-Layer Engineered Flexible Functional Film Fabrication with Spreadability Control in Roll-to-Roll Manufacturing
Authors: Jeon Hojin , Noh Jaehyun , Jo Minho , Joo Changbeom , Jo Jeongdai , Lee Changwoo
Published: May 2023

A roll-to-roll manufacturing system performs printing and coating on webs to mass-produce large-area functional films. The functional film of a multilayered structure is composed of layers with different components for performance improvement. The roll-to-roll system is capable of controlling the geometries of the coating and printing layers using process variables. However, research on geometric control using process variables is limited to single-layer structures only. This study entails the development of a method to proactively control the geometry of the upper coated layer by using the lower-layer coating process variable in the manufacture of a double-coated layer. The correlation between the lower-layer coating process variable and upper coated layer geometry was examined by analyzing the lower-layer surface roughness and spreadability of the upper-layer coating ink. The correlation analysis results demonstrate that tension was the dominant variable in the upper coated layer surface roughness. Additionally, this study found that adjusting the process variable of the lower-layer coating in a double-layered coating process could improve the surface roughness of the upper coating layer by up to 14.9%.
Graphene Oxide Facilitates Transformation of Waste PET into MOF Nanorods in Ionic Liquids
Authors: Gangaraju Deepa , Shanmugharaj Andikkadu Masilamani , Sridhar Vadahanambi
Published: May 2023

Although though ionic liquids (IL) are rapidly emerging as highly efficient reagents for the depolymerization of waste plastics, their high cost and adverse impact on the environment make the overall process not only expensive but also environmentally harmful. In this manuscript, we report that graphene oxide (GO) facilitates the transformation of waste polyethylene terephthalate (PET) to Ni-MOF (metal organic framework) nanorods anchored on reduced graphene oxide (Ni–MOF@rGO) through NMP (N-Methyl-2-pyrrolidone)-based coordination in ionic liquids. Morphological studies using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed mesoporous three-dimensional structures of micrometer-long Ni-MOF nanorods anchored on reduced graphene substrates (Ni–MOF@rGO ), whereas structural studies using XRD and Raman spectra demonstrated the crystallinity of Ni-MOF nanorods. Chemical analysis of Ni–MOF@rGO carried out using X-ray photoelectron spectroscopy demonstrated that nickel moieties exist in an electroactive OH-Ni-OH state, which was further confirmed by nanoscale elemental maps recorded using energy-dispersive X-ray spectroscopy (EDS). The applicability of Ni–MOF@rGO as an electro-catalyst in a urea-enhanced water oxidation reaction (UOR) is reported. Furthermore, the ability of our newly developed NMP-based IL to grow MOF nanocubes on carbon nanotubes and MOF nano-islands on carbon fibers is also reported.