bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Results of a Screening Programme for Chlamydia in an Asymptomatic Young Population in Spain
Authors: Yuguero Oriol , Fernández-Armenteros Jose Manuel , Vilela Álvaro , Aramburu Jesús , Laín Raquel , Godoy Pere
Published: February 2021

Introduction:Chlamydia trachomatis (CT) infection has increased in recent years, reaching 127 million cases in 2016. Possible complications, especially among women, require intervention for early detection of the infection. The objective of our study was to determine the prevalence of CT infection in a young, sexually active, asymptomatic population.

Methods: A cross-sectional study was conducted between December 2017 and 31 December 2018 among young patients aged 18–25 years attending the emergency room for any reason. The presence of CT and other STIs in urine was determined by the Allplex Nucleic Acid Amplification Test (NAAT) with a urine sample. All patients testing positive were followed by the STD unit and tests on all sexual partners/contacts were offered. Moreover, we obtained data about sexual habits and risk factors via a self-reporting questionnaire.

Results: One thousand three hundred eight patients were eligible for inclusion of whom 298 consented to participate. Of these, 22/298 (7.4%) were diagnosed with CT. Young people with two or more sexual partners in the last month and those suffering from infection by ureaplasma were at greater risk of infection by CT. Up to 50% of participants do not use barrier methods.

Conclusion: The prevalence of infection by CT in the asymptomatic young population is higher than expected according to the recent literature in Spain. The scarce use of barrier methods among this population may be one of the causes of this increase and one of the targets to work on in order to reduce the prevalence of the infection.
Unemployment and Health-Related Quality of Life in Melanoma Patients During the COVID-19 Pandemic
Authors: Guo Yeye , Shen Minxue , Zhang Xu , Xiao Yi , Zhao Shuang , Yin Mingzhu , Bu Wenbo , Wang Yan , Chen Xiang , Su Juan
Published: February 2021

The outbreak of coronavirus disease-2019 (COVID-19) ineluctably caused social distancing and unemployment, which may bring additional health risks for patients with cancer. To investigate the association of the pandemic-related impacts with the health-related quality of life (HRQoL) among patients with melanoma during the COVID-19 pandemic, we conducted a cross-sectional study among Chinese patients with melanoma. A self-administered online questionnaire was distributed to melanoma patients through social media. Demographic and clinical data, and pandemic-related impacts (unemployment and income loss) were collected. HRQoL was determined by the Functional Assessment of Cancer Therapy-General (FACT-G) and its disease-specific module (the melanoma subscale, MS). A total of 135 patients with melanoma completed the study. The mean age of the patients was 55.8 ± 14.2 years, 48.1% (65/135) were male, and 17.04% (34/135) were unemployed since the epidemic. Unemployment of the patients and their family members and income loss were significantly associated with a lower FACT-G score, while the MS score was associated with the unemployment of the patients' family members. Our findings suggested that unemployment is associated with impaired HRQoL in melanoma patients during the COVID-19 epidemic.
DETECT Schools Study Protocol: A Prospective Observational Cohort Surveillance Study Investigating the Impact of COVID-19 in Western Australian Schools
Authors: Mullane Marianne J. , Thomas Hannah M. , Epstein Melanie , Mandzufas Joelie , Mullan Narelle , Whelan Alexandra , Lombardi Karen , Barrow Tina , Ang Sherlynn , Leahy Adele , Cameron Ewan , Lester Leanne , Cooper Matt , Stevenson Paul , Hartman Mitch , McKenzie Anne , Mitrou Francis , Zubrick Stephen R. , Coffin Juli , Cross Donna , Bowen Asha C. , Gething Peter
Published: February 2021

Introduction: Amidst the evolving COVID-19 pandemic, understanding the transmission dynamics of the SARS-CoV-2 virus is key to providing peace of mind for the community and informing policy-making decisions. While available data suggest that school-aged children are not significant spreaders of SARS-CoV-2, the possibility of transmission in schools remains an ongoing concern, especially among an aging teaching workforce. Even in low-prevalence settings, communities must balance the potential risk of transmission with the need for students' ongoing education. Through the roll out of high-throughput school-based SARS-CoV-2 testing, enhanced follow-up for individuals exposed to COVID-19 and wellbeing surveys, this study investigates the dynamics of SARS-CoV-2 transmission and the current psychosocial wellbeing impacts of the pandemic in school communities.

Methods: The DETECT Schools Study is a prospective observational cohort surveillance study in 79 schools across Western Australia (WA), Australia. To investigate the incidence, transmission and impact of SARS-CoV-2 in schools, the study comprises three “modules”: Module 1) Spot-testing in schools to screen for asymptomatic SARS-CoV-2; Module 2) Enhanced surveillance of close contacts following the identification of any COVID-19 case to determine the secondary attack rate of SARS-CoV-2 in a school setting; and Module 3) Survey monitoring of school staff, students and their parents to assess psycho-social wellbeing following the first wave of the COVID-19 pandemic in WA.

Clinical Trial Registration: Trial registration number: ACTRN12620000922976
Inflammatory Mediators in Oral Cancer: Pathogenic Mechanisms and Diagnostic Potential
Authors: Niklander Sven E.
Published: February 2021

Approximately 15% of cancers are attributable to the inflammatory process, and growing evidence supports an association between oral squamous cell carcinoma (OSCC) and chronic inflammation. Different oral inflammatory conditions, such as oral lichen planus (OLP), submucous fibrosis, and oral discoid lupus, are all predisposing for the development of OSCC. The microenvironment of these conditions contains various transcription factors and inflammatory mediators with the ability to induce proliferation, epithelial-to-mesenchymal transition (EMT), and invasion of genetically predisposed lesions, thereby promoting tumor development. In this review, we will focus on the main inflammatory molecules and transcription factors activated in OSCC, with emphasis on their translational potential.
Phenotypic Responses to Selection for Ultrafine Wool in Uruguayan Yearling Lambs
Authors: Ramos Zully , Blair Hugh Thomas , De Barbieri Ignacio , Ciappesoni Gabriel , Montossi Fabio , Kenyon Paul Richard
Published: February 2021

This study evaluated the phenotypic trends for wool and growth traits of the fine Merino genetic nucleus in Uruguay. Data were collected from one-year-old lambs over a twenty-year period (1999–2018). The overall aim of the selection flock was to reduce fiber diameter with concomitant increases in fleece and live weights. Traits analyzed included fiber diameter (FD), greasy fleece weight (GFW), coefficient of variation of FD (CVfd), staple length (SL), scoured yield (SY), live weight post-shearing (LW), eye muscle area (EMA) and fat thickness (FAT). Data from approximately 5300 one-year-old male and female lambs were analyzed. During the study period, FD decreased by approximately 3 µm, whereas GFW and LW increased by at least 0.5 and 3.0 kg, respectively. There were interactions between the sex of the individual and the year for all wool traits. Except for FAT, all other traits were affected by the dam age. This study indicates that the selection program applied in the fine Merino genetic nucleus over a twenty-year period resulted in reductions in FD and increases in GFW and LW. Therefore, the results indicate it is possible to produce ultrafine wool in semi-extensive grazing systems without compromising other economically relevant traits in one-year-old lambs.
Resistance of European Spring 2-Row Barley Cultivars to Pyrenophora graminea and Detection of Associated Loci
Authors: Faccini Nadia , Delbono Stefano , Çelik Oğuz Arzu , Cattivelli Luigi , Valè Giampiero , Tondelli Alessandro
Published: February 2021

Pyrenophora graminea is the seed-borne pathogen causal agent of barley leaf stripe disease. In this work, we screened a collection of 206 spring two-row barley cultivars from Europe for their resistance to the fungal pathogen. Artificial inoculation with the highly virulent isolate Dg2 revealed a continuous variation for the incidence of infection, with few highly resistant or highly susceptible genotypes. On average, old cultivars showed higher resistance than the more modern ones. Genome-Wide Association Scan was performed by exploiting available molecular data for >4000 SNP markers and revealed a single, highly significant association on the short arm of chromosome 6H, in a genomic position where quantitative trait loci (QTL) for barley resistance to P. graminea were not detected before. Based on the last version of the reference barley genome, genes encoding for proteins with a kinase domain were suggested as candidates for the locus.
Using Regulatory Flexibility to Address Market Informality in Seed Systems: A Global Study
Authors: Kuhlmann Katrin , Dey Bhramar
Published: February 2021

Seed rules and regulations determine who can produce and sell seeds, which varieties will be available in the market, the quality of seed for sale, and where seed can be bought and sold. The legal and regulatory environment for seed impacts all stakeholders, including those in the informal sector, through shaping who can participate in the market and the quality and diversity of seed available. This paper addresses a gap in the current literature regarding the role of law and regulation in linking the informal and formal seed sectors and creating more inclusive and better governed seed systems. Drawing upon insights from the literature, global case studies, key expert consultations, and a methodology on the design and implementation of law and regulation, we present a framework that evaluates how regulatory flexibility can be built into seed systems to address farmers’ needs and engage stakeholders of all sizes. Our study focuses on two key dimensions: extending market frontiers and liberalizing seed quality control mechanisms. We find that flexible regulatory approaches and practices play a central role in building bridges between formal and informal seed systems, guaranteeing quality seed in the market, and encouraging market entry for high-quality traditional and farmer-preferred varieties.
Influence of Gonadectomy on Canine Behavior
Authors: Palestrini Clara , Mazzola Silvia Michela , Caione Bianca , Groppetti Debora , Pecile Alessandro M. , Minero Michela , Cannas Simona
Published: February 2021

Due to the lack of unequivocal scientific evidence, gonadectomy’s effects on dogs’ behavior are still debated. Since veterinarians differ in their opinion, there may be considerable diversity in the advice received by owners. This study aimed to evaluate the effects of gonadectomy on dog behavior across time. Ninety-six dog owners (48 control dogs and 48 experimental dogs) were interviewed twice (T0 and T1, nine months later) to obtain information about their dog’s behavior. No change was found in the eating behavior or weight of dogs of both groups. Compared to T0, at T1, experimental dogs were reported to show less mounting behavior, pull on the leash, and roaming behaviors. Marking behavior did not vary across time for both groups of dogs. A tendency to reduce owner-directed aggression was observed at T1 for experimental male dogs, while no change was observed for male controls. The literature reports conflicting information about the effect of gonadectomy on behavior, suggesting that further studies about this topic should be undertaken.
Exploring How White-Faced Sakis Control Digital Visual Enrichment Systems
Authors: Hirskyj-Douglas Ilyena , Kankaanpää Vilma
Published: February 2021

Computer-enabled screen systems containing visual elements have long been employed with captive primates for assessing preference, reactions and for husbandry reasons. These screen systems typically play visual enrichment to primates without them choosing to trigger the system and without their consent. Yet, what videos primates, especially monkeys, would prefer to watch of their own volition and how to design computers and methods that allow choice is an open question. In this study, we designed and tested, over several weeks, an enrichment system that facilitates white-faced saki monkeys to trigger different visual stimuli in their regular zoo habitat while automatically logging and recording their interaction. By analysing this data, we show that the sakis triggered underwater and worm videos over the forest, abstract art, and animal videos, and a control condition of no-stimuli. We also note that the sakis used the device significantly less when playing animal videos compared to other conditions. Yet, plotting the data over time revealed an engagement bell curve suggesting confounding factors of novelty and habituation. As such, it is unknown if the stimuli or device usage curve caused the changes in the sakis interactions over time. Looking at the sakis’ behaviours and working with zoo personnel, we noted that the stimuli conditions resulted in significantly decreasing the sakis’ scratching behaviour. For the research community, this study builds on methods that allow animals to control computers in a zoo environment highlighting problems in quantifying animal interactions with computer devices.
LC-MS/MS Based Metabolomics Reveal Candidate Biomarkers and Metabolic Changes in Different Buffalo Species
Authors: Shi Wen , Yuan Xiang , Cui Kuiqing , Li Hui , Fu Penghui , Rehman Saif-Ur , Shi Deshun , Liu Qingyou , Li Zhipeng
Published: February 2021

Consumers have shown more and more interest in high-quality and healthy dairy products and buffalo milk is commercially more viable than other milks in producing superior dairy products due to its higher contents of fat, crude protein, and total solids. Metabolomics is one of the most powerful strategies in molecular mechanism research however, little study has been focused on the milk metabolites in different buffalo species. Therefore, the aim of this study was to explore the underlying molecular mechanism of the fatty synthesis and candidate biomarkers by analyzing the metabolomic profiles. Milk of three groups of buffaloes, including 10 Mediterranean, 12 Murrah, and 10 crossbred buffaloes (Murrah × local swamp buffalo), were collected and UPLC-Q-Orbitrap HRMS was used to obtain the metabolomic profiles. Results showed that milk fatty acid in Mediterranean buffalo was significantly higher than Murrah buffalo and crossbred buffalo. A total of 1837/726 metabolites was identified in both positive and negative electrospray ionization (ESI±) mode, including 19 significantly different metabolites between Mediterranean and Murrah buffalo, and 18 different metabolites between Mediterranean and crossbred buffalo. We found 11 of the different metabolites were both significantly different between Mediterranean vs. Murrah group and Mediterranean vs crossbred group, indicating that they can be used as candidate biomarkers of Mediterranean buffalo milk. Further analysis found that the different metabolites were mainly enriched in fat synthesis related pathways such as fatty acid biosynthesis, unsaturated fatty acid biosynthesis, and linoleic acid metabolism, indicating that the priority of different pathways affected the milk fat content in different buffalo species. These specific metabolites may be used as biomarkers in the identification of milk quality and molecular breeding of high milk fat buffalo.
Spatiotemporal Overlap between the European Brown Hare and Its Potential Predators and Competitors
Authors: Viviano Andrea , Mori Emiliano , Fattorini Niccolò , Mazza Giuseppe , Lazzeri Lorenzo , Panichi Alessandra , Strianese Luigi , Mohamed Walid Fathy
Published: February 2021

Analysis of spatiotemporal partitioning is pivotal to shed light on interspecific coexistence. Most research effort has involved large-sized carnivores and their prey, whereas little attention has been devoted to lagomorphs. We assessed spatiotemporal overlap among the European brown hare Lepus europaeus and its potential competitors and predators through camera-trapping in an area in Central Italy. We estimated the interspecific patterns of the spatiotemporal activity rhythms of brown hares, its potential predators (the red fox Vulpes vulpes, the pine marten Martes martes, the domestic cat Felis catus, and the domestic dog Canis familiaris), and a competitor, the roe deer Capreolus capreolus. Brown hare activity was studied in natural conditions as well as in a fenced area that excluded terrestrial predators and competitors. Free-ranging hares developed a more nocturnal behavior to avoid diurnal predators (i.e., domestic carnivores and martens). Although high temporal overlap was observed between free-ranging brown hares and both red foxes (82%) and roe deer (81%), hares avoided fox by being more active on darkest nights, as well as avoided roe deer through spatial partitioning. We suggest that hares may adapt their spatiotemporal behavior to avoid potential predators and competitors.
Endocrine Signals Altered by Heat Stress Impact Dairy Cow Mammary Cellular Processes at Different Stages of the Dry Period
Authors: Ouellet Véronique , Negrao João , Skibiel Amy L. , Lantigua Valerie A. , Fabris Thiago F. , Marrero Marcela G. , Dado-Senn Bethany , Laporta Jimena , Dahl Geoffrey E.
Published: February 2021

Hormonal alterations occurring under late gestation heat stress may disturb mammary gland remodelling, resulting in a reduced milk yield during the subsequent lactation. We investigated the effects of an altered endocrine environment on mammary gene expression at different stages of the dry period. Mammary gland biopsies from in vivo-cooled (CL) or heat-stressed (HT) cows were collected at d 3 and 35 relative to dry-off and divided into explants. Explants were incubated in vitro for 24 h in one of three media: Basal: no prolactin or estrogen; CL-mimic: Basal + low prolactin + high 17β-estradiol, or HT-mimic: Basal + high prolactin + low 17β-estradiol. Real time qPCR was used to quantify gene expression. We established that late-gestation heat stress changes the expression of prolactin and oestrogen receptors, downregulates genes involved in apoptosis, autophagy and proliferation at d 3 and upregulates genes related to those cellular processes at d 35. Moreover, compared with in vivo treatments, we showed that the expression of fewer genes was impacted by in vitro treatments which aimed to mimic the hormonal response of cows exposed to a different environment. Further research will continue to uncover the mechanisms behind the production impairments caused by late-gestation heat stress.
Feed Quality and Feeding Level Effects on Faecal Composition in East African Cattle Farming Systems
Authors: Ali Asep I. M. , Wassie Shimels E. , Joergensen Rainer Georg , Korir Daniel , Goopy John P. , Butterbach-Bahl Klaus , Merbold Lutz , Dickhoefer Uta , Schlecht Eva
Published: February 2021

Effects of feeding levels below maintenance requirements of metabolizable energy (MER) and of feed supplementation on fecal nutrient and microbial C concentrations were evaluated. In experiment 1, Rhodes grass hay only was offered to Boran steers at 80%, 60%, and 40% of individual MER, while steers at 100% MER additionally received a concentrated mixture. This reduction in MER decreased N, increased fungal C but did not affect bacterial C concentrations in feces. In experiment 2, Holstein × Boran heifers were offered a poor-quality roughage diet without supplement, with sweet potato vine silage or with a urea-molasses block. These two supplements did not affect the fecal chemical composition or fungal C but increased bacterial C concentrations in feces. Across all data, the fungal C/bacterial C ratio was positively related to N and negatively to neutral detergent fiber concentrations in feces, indicating diet-induced shifts in the fecal microbial community.
Effects of Decabrominated Diphenyl Ether Exposure on Growth, Meat Characteristics and Blood Profiles in Broilers
Authors: Liu Zehui , Lei Hulong , Tang Renyong , Yang Junhua , Guo Xiulan , Huang Renmao , Rao Qinxiong , Cheng Lin , Zhao Zhihui
Published: February 2021

Decabrominated diphenyl ether (BDE-209) is widely used as a flame retardant and is detected at high levels in the environment. Its toxicities have been reported and have attracted attention. In the present study, broilers were used to determine the response in growth performance, carcass traits, meat quality, blood profiles and antioxidant system to BDE-209 exposure at doses of 0, 0.02, 0.4 and 4 mg/kg. The results showed that BDE-209 exposure at levels of 0.02 or 0.4 mg/kg increased feed intake and decreased feed efficiency. BDE-209 altered the blood profiles, such as reducing the numbers of white blood cells, lymphocytes and neutrophilic granulocytes. As compared with the control, BDE-209 exposure significantly increased abdominal fat percentages of broilers at 64.9–159.5% and adversely affected the selected biochemical indicators, including alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine (CRE), which indicated its toxicity to liver and kidney functions. Moreover, BDE-209 exposure significantly increased plasma malondialdehyde (MDA) concentrations and decreased the activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD), which implied aggravating oxidant stress and decline of antioxidant capacity in broilers. In conclusion, our data demonstrated that the environmental pollutant BDE-209 adversely influenced growth performance, increased the deposition of abdominal fat, impaired antioxidant capacity and the immune system and had potential toxicity to the liver and kidney of broilers.
A New Limnonectes (Anura: Dicroglossidae) from Southern Thailand
Authors: Yodthong Siriporn , Rujirawan Attapol , Stuart Bryan L. , Aowphol Anchalee
Published: February 2021

A new species in the dicroglossid frog genus Limnonectes is described from Ko Pha-ngan, Ko Samui, and Ko Lanta Yai Islands in southern Thailand. Males of Limnonectes pseudodoriae sp. nov. lack a caruncle on top of the head and very closely resemble L. doriae (Boulenger, 1887) from Myanmar and western and southern Thailand. However, the new species is distinguished from L. doriae and its congeners using an integrative taxonomic approach of morphology, mitochondrial DNA, and bioacoustics. Limnonectes pseudodoriae sp. nov. differs from L. doriae and its congeners by having a unique combination of morphological characters, including body size; skin texture of the interorbital region, dorsum, and shank; toe webbing; relative size of the inner metatarsal tubercle; and coloration of the tympanum, venter, and ova. The advertisement call of the new species is also readily differentiated from that of L. doriae in temporal parameters. Limnonectes pseudodoriae sp. nov. is highly divergent in mitochondrial DNA from L. doriae and its congeners, but its phylogenetic position within the genus is not resolved. The natural history of the new species is presented, and the geographic range of L. doriae in Thailand is clarified.
Groups and Individuals: Optical Flow Patterns of Broiler Chicken Flocks Are Correlated with the Behavior of Individual Birds
Authors: Gebhardt-Henrich Sabine G. , Stratmann Ariane , Dawkins Marian Stamp
Published: February 2021

Group level measures of welfare flocks have been criticized on the grounds that they give only average measures and overlook the welfare of individual animals. However, we here show that the group-level optical flow patterns made by broiler flocks can be used to deliver information not just about the flock averages but also about the proportion of individuals in different movement categories. Mean optical flow provides information about the average movement of the whole flock while the variance, skew and kurtosis quantify the variation between individuals. We correlated flock optical flow patterns with the behavior and welfare of a sample of 16 birds per flock in two runway tests and a water (latency-to-lie) test. In the runway tests, there was a positive correlation between the average time taken to complete the runway and the skew and kurtosis of optical flow on day 28 of flock life (on average slow individuals came from flocks with a high skew and kurtosis). In the water test, there was a positive correlation between the average length of time the birds remained standing and the mean and variance of flock optical flow (on average, the most mobile individuals came from flocks with the highest mean). Patterns at the flock level thus contain valuable information about the activity of different proportions of the individuals within a flock.
Polymorphism Detection of GDF9 Gene and Its Association with Litter Size in Luzhong Mutton Sheep (Ovis aries)
Authors: Wang Fengyan , Chu Mingxing , Pan Linxiang , Wang Xiangyu , He Xiaoyun , Zhang Rensen , Tao Lin , La Yongfu , Ma Lin , Di Ran
Published: February 2021

Litter size is one of the most important economic traits in sheep. GDF9 and BMPR1B are major genes affecting the litter size of sheep. In this study, the whole coding region of GDF9 was sequenced and all the SNPs (single nucleotide polymorphisms) were determined in Luzhong mutton ewes. The FecB mutation was genotyped using the Sequenom MassARRAY®SNP assay technology. Then, the association analyses between polymorphic loci of GDF9 gene, FecB, and litter size were performed using a general linear model procedure. The results showed that eight SNPs were detected in GDF9 of Luzhong mutton sheep, including one novel mutation (g.41769606 T > G). The g.41768501A > G, g.41768485 G > A in GDF9 and FecB were significantly associated with litter size in Luzhong mutton ewes. The g.41768485 G > A is a missense mutation in the mature GDF9 protein region and is predicted to affect the tertiary structure of the protein. The results preliminarily demonstrated that GDF9 was a major gene affecting the fecundity of Luzhong mutton sheep and the two loci g.41768501A > G and g.41768485 G > A may be potential genetic markers for improving litter size.
Effects of Replacing Ensiled-Alfalfa with Fresh-Alfalfa on Dynamic Fermentation Characteristics, Chemical Compositions, and Protein Fractions in Fermented Total Mixed Ration with Different Additives
Authors: Gao Run , Luo Ying , Xu Shengyang , Wang Musen , Sun Zhiqiang , Wang Lei , Yu Zhu
Published: February 2021

Alfalfa (Medicago sativa) is one of the high protein ingredients of fermented total mixed ration (FTMR). Additionally, FTMR is widely used to satisfy the nutrition requirements of animals. This study was conducted to confirm the fermentation characteristics, chemical compositions and protein fractions changes when replacing ensiled-alfalfa with fresh-alfalfa in FTMR with additives. Three additives were separately applied to fresh-alfalfa total mixed ration (TMR) and ensiled-alfalfa TMR, including molasses (MOL), Lactobacillus plantarum (LP) and MOL plus LP (MOL+LP). The same volume of distilled water was sprayed onto the prepared TMR as performed for the control (CK). Each treatment included 18 repetitions and opened 3 repetitions at each fermenting day (1, 3, 7, 15, 30 and 60 d). The results showed that fresh-alfalfa FTMR (F-FTMR) exhibited slight changes in the fermentation characteristics during the first 7 d and showed similar trends in terms of the pH and organic acids content to ensiled-alfalfa FTMR (E-FTMR). The lactic acid contents of F-FTMR were significantly lower than those of E-FTMR at 60 d fermentation and the ammonia nitrogen contents were lower than E-FTMR during the entire fermenting period. The crude protein of the F-FTMR was enhanced after 60 d of fermenting. F-FTMR supplemented with MOL+LP exhibited a lower nonprotein nitrogen content, variable to slow protein and indigestible protein contents, and higher fast degradable protein and true protein degraded intermediately contents at 60 d fermenting, indicating that it effectively inhibited protein degradation.
Isolation and Characterization of Antibacterial Carotane Sesquiterpenes from Artemisia argyi Associated Endophytic Trichoderma virens QA-8
Authors: Shi Xiao-Shan , Song Yin-Ping , Meng Ling-Hong , Yang Sui-Qun , Wang Dun-Jia , Zhou Xing-Wang , Ji Nai-Yun , Wang Bin-Gui , Li Xiao-Ming
Published: February 2021

Carotane sesquiterpenes are commonly found in plants but are infrequently reported in the fungal kingdom. Chemical investigation of Trichoderma virens QA-8, an endophytic fungus associated with the inner root tissue of the grown medicinal herb Artemisia argyi H. Lév. and Vaniot, resulted in the isolation and characterization of five new carotane sesquiterpenes trichocarotins I–M (1–5), which have diverse substitution patterns, and seven known related analogues (6–12). The structures of these compounds were established on the basis of a detailed interpretation of their NMR and mass spectroscopic data, and the structures including the relative and absolute configurations of compounds 1–3, 5, 9, and 10 were confirmed by X-ray crystallographic analysis. In the antibacterial assays, all isolates exhibited potent activity against Escherichia coli EMBLC-1, with MIC values ranging from 0.5 to 32 µg/mL, while 7β-hydroxy CAF-603 (7) strongly inhibited Micrococcus luteus QDIO-3 (MIC = 0.5 µg/mL). Structure-activity relationships of these compounds were discussed. The results from this study demonstrate that the endophytic fungus T. virens QA-8 from the planted medicinal herb A. argyi is a rich source of antibacterial carotane sesquiterpenes, and some of them might be interesting for further study to be developed as novel antibacterial agents.
Toward Safe Pharmacotherapy: The Interplay between Meropenem and Parenteral Nutrition Admixtures
Authors: Gostyńska Aleksandra , Piwowarczyk Ludwika , Nadolna Malwina , Jelińska Anna , Dettlaff Katarzyna , Ogrodowczyk Magdalena , Popielarz-Brzezińska Maria , Stawny Maciej
Published: February 2021

Simultaneous administration of parenteral nutrition (PN) admixtures with intravenous antibiotics is a common clinical problem. Coadministration of drugs incompatible with PN admixture may affect its stability, especially in the context of lipid droplet size, which is a crucial parameter for patient safety. In the present study, we investigate the in vitro compatibility of meropenem (Meropenem 1000, MPM) with five commercial PN admixtures used worldwide: Kabiven, Olimel N9E, Nutriflex Lipid Special, Nutriflex Omega Special, and SmofKabiven. The appropriate volumetric ratios, reflecting their clinical practice ratios, were used to prepare the MPM–PN admixture samples. Physicochemical properties of MPM–PN admixtures samples were determined upon preparation and after four hours of storage. The pH changes for all MPM–PN admixtures samples did not exceed the assumed level of acceptability and ranged from 6.41 to 7.42. After four hours of storage, the osmolarity changes were ±3%, except MPM–Olimel N9E samples, for which differences from 7% to 11% were observed. The adopted level of acceptability of changes in zeta potential after four hours of storage (±3 mV) was met for MPM–Kabiven, MPM–Nutriflex Lipid Special, and MPM–Nutriflex Omega Special. The mean droplet diameter for all samples was below 500 nm. However, only in the case of Nutriflex Lipid Special and Nutriflex Omega Special, the addition of MPM did not cause the formation of the second fraction of lipid droplets. The coadministration of MPM via Y-site with Kabiven, Olimel N9E, and Smofkabiven should be avoided due to osmolarity fluctuations (MPM–Olimel), significant differences in zeta potential (MPM–Olimel, MPM–Smofkabiven), and the presence of the second fraction of lipid droplets >1000 nm (MPM–Kabiven, MPM–Olimel, and MPM–Smofkabiven). The assumed acceptance criteria for MPM compatibility of MPM with PN admixtures were met only for Nutriflex Lipid Special and Nutriflex Omega Special in 1:1, 2:1, and 4:1 volume ratios.
Biochemical Regulation of the Glyoxalase System in Response to Insulin Signaling
Authors: Lee Der-Yen , Lin Yu-Chin , Chang Geen-Dong
Published: February 2021

Methylglyoxal (MG) is a reactive glycation metabolite and potentially induces dicarbonyl stress. The production of MG in cells is increased along with an increase in carbohydrate metabolism. The efficiency of the glyoxalase system, consisting of glyoxalase 1 (GlxI) and glyoxalase 2 (GlxII), is crucial for turning the accumulated MG into nontoxic metabolites. Converting MG-glutathione hemithioacetal to S-d-lactoylglutathione by GlxI is the rate-determining step of the enzyme system. In this study, we found lactic acid accumulated during insulin stimulation in cells, however, cellular MG and S-d-lactoylglutathione also increased due to the massive flux of glycolytic intermediates. The insulin-induced accumulation of MG and S-d-lactoylglutathione were efficiently removed by the treatment of metformin, possibly via affecting the glyoxalase system. With the application of isotopic 13C3-MG, the flux of MG from extracellular and intracellular origins was dissected. While insulin induced an influx of extracellular MG, metformin inhibited the trafficking of MG across the plasma membrane. Therefore, metformin could maintain the extracellular MG by means of reducing the secretion of MG rather than facilitating the scavenging. In addition, metformin may affect the glyoxalase system by controlling the cellular redox state through replenishing reduced glutathione. Overall, alternative biochemical regulation of the glyoxalase system mediated by insulin signaling or molecules like biguanides may control cellular MG homeostasis.
Comparative Study Regarding the Chemical Composition and Biological Activity of Pine (Pinus nigra and P. sylvestris) Bark Extracts
Authors: Nisca Adrian , Ștefănescu Ruxandra , Stegăruș Diana Ionela , Mare Anca Delia , Farczadi Lenard , Tanase Corneliu
Published: February 2021

The bark resulted after the industrial processing of wood represents a byproduct of the forestry industry, used in many suboptimal ways, being considered a natural waste. Currently, it has been highlighted that the bark of different woody plants may be an important source of several bioactive compounds, with various beneficial biological functions. The aim of this study is to evaluate and compare the chemical composition and biological activities of two different pine species (Pinus nigra and Pinus sylvestris) bark extracts. Ultrasound (UAE) and microwave (MAE) assisted extractions were performed in order to obtain the extracts. The total polyphenol (TPC) and total tannin (TTC) contents of the extracts were assessed via the Folin–Ciocâlteu method. The volatile and polyphenolic compounds were identified and quantified via a GC/MS analysis and an UPLC-PDA analysis, respectively. The antioxidant activity (AOA) was evaluated using the DPPH and ABTS assays, while the antibacterial activity was assessed using the minimum inhibitory concentration (MIC) protocol. The results showed that the Pinus sylvestris bark extracts obtained by UAE had a higher TPC, TTC and AOA, while the Pinus nigra bark extracts obtained by MAE had higher volatile compounds content (mainly α-pinene and β-pinene). Moreover, the inhibition of the bacterial growth was more efficient in the case of Pinus sylvestris extracts, Gram-positive bacteria being the most affected, while Gram-negative strains presented a relative resistance to the tested extract concentrations. These results may indicate the potential use of the pine bark extracts as antioxidant or antibacterial agents.
Efficacy and Effectiveness of Physical Agent Modalities in Complex Regional Pain Syndrome Type I: A Scoping Review
Authors: Moretti Antimo , Gimigliano Francesca , Paoletta Marco , Liguori Sara , Toro Giuseppe , Aulicino Milena , Conversano Stefano , Iolascon Giovanni
Published: February 2021

Complex regional pain syndrome type I (CRPS-I) is a rare condition with huge variability in triggering factors and clinical scenarios. The complexity of the pathophysiology of this condition fosters the proposal of several therapeutic options with different mechanisms of action in both research and clinical practice. An interdisciplinary and multimodal approach, including pharmacological and non-pharmacological interventions, particularly physical therapy, is recommended by international guidelines, but the benefits and harms of available interventions are poorly known. In this scoping review, the clinical rationale for use of physical agent modalities for patients with CRPS-I will be presented. We found 10 studies addressing the role of electromagnetic field therapy, electrotherapy, and laser therapy. Our findings suggest that physical therapy modalities, in particular transcutaneous electrical nerve stimulation (TENS) and pulsed electromagnetic field therapy (PEMF), may contribute to reduce pain and improve function in patients with CRPS-1.
CFD Analysis of a Tubular Heat Exchanger for the Conditioning of Olive Paste
Authors: Perone Claudio , Romaniello Roberto , Leone Alessandro , Catalano Pasquale , Tamborrino Antonia
Published: February 2021

The use of a heat exchanger for the conditioning of the olive paste could enhance the olive oil extraction process. Particularly, paste pre-heating could reduce the malaxation time and, most of all, improve the temperature control during this process (e.g., 27 °C). In this study, a three-dimensional computational fluid dynamics (CFD) analysis of a tubular heat exchanger was carried out to better understand the influence of the inlet conditions of the olive paste on thermal and hydrodynamic behavior within it. CFD analysis was performed with SOLIDWORKS Flow Simulation (ver.2016). The heat exchanger consists of a tube-in-tube module, in which the inner tube was fed with the olive paste, while the jacket was filled of hot water. The main aim was that to predict the heat transfer and pressure drop in paste side of the exchanger. Multiple analyses by varying the mass flow rate and inlet temperature of the paste were carried out, and temperature and pressure drop were estimated. The numerical model has proved very useful in identifying the main factors affecting the optimization of the heat exchanger in order to improve the extraction process of the olive paste.
Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils
Authors: Nebeská Diana , Auer Malinská Hana , Erol Anna , Pidlisnyuk Valentina , Kuráň Pavel , Medžová Andrea , Smaha Martin , Trögl Josef
Published: February 2021

Second-generation biofuel crop miscanthus is one of the most promising plants tested for phytomanagement of contaminated sites. In this preliminary pot case study, the most used hybrid Miscanthus x giganteus was cultivated in three different real contaminated soils: agricultural soil contaminated with Cd; post-military soil slightly contaminated with Zn, Pb and Cd; and soil contaminated by petroleum industry with metals and hydrocarbons. The stress response of plants and soil microbial communities was monitored to receive data that are important for successful phytomanagement application. With metals only, the plant grew well, and chlorophyll fluorescence measurement proved their good vitality. Changes in leaf anatomy (leaf thickness and sclerenchyma cells area) were additionally determined in post-military soil compared to agricultural. On the contrary, in petroleum-contaminated soil, the biomass yield was too reduced and also physiological parameters were significantly decreased. The response of microbial communities also differed. In agricultural soil, no microbial stress was determined. In post-military soil, it became reduced during the experiment, and in petroleum contamination, it increased year-on-year. It could be concluded that miscanthus is suitable for cultivation in metals contaminated soils with potential for microbial communities support, but in soil contaminated by the petroleum industry, its application did not seem meaningful.
Schiff Bases: Interesting Scaffolds with Promising Antitumoral Properties
Authors: Iacopetta Domenico , Ceramella Jessica , Catalano Alessia , Saturnino Carmela , Bonomo Maria Grazia , Franchini Carlo , Sinicropi Maria Stefania
Published: February 2021

Schiff bases, named after Hugo Schiff, are highly reactive organic compounds broadly used as pigments and dyes, catalysts, intermediates in organic synthesis, and polymer stabilizers. Lots of Schiff bases are described in the literature for various biological activities, including antimalarial, antibacterial, antifungal, anti-inflammatory, and antiviral. Schiff bases are also known for their ability to form complexes with several metals. Very often, complexes of Schiff bases with metals and Schiff bases alone have demonstrated interesting antitumor activity. Given the innumerable vastness of data regarding antitumor activity of all these compounds, we focused our attention on mono- and bis-Schiff bases alone as antitumor agents. We will highlight the most significant examples of compounds belonging to this class reported in the literature.
Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials
Authors: Bush Joshua , Hu Chih-Hsiang , Veneziano Remi
Published: February 2021

DNA hydrogels are self-assembled biomaterials that rely on Watson–Crick base pairing to form large-scale programmable three-dimensional networks of nanostructured DNA components. The unique mechanical and biochemical properties of DNA, along with its biocompatibility, make it a suitable material for the assembly of hydrogels with controllable mechanical properties and composition that could be used in several biomedical applications, including the design of novel multifunctional biomaterials. Numerous studies that have recently emerged, demonstrate the assembly of functional DNA hydrogels that are responsive to stimuli such as pH, light, temperature, biomolecules, and programmable strand-displacement reaction cascades. Recent studies have investigated the role of different factors such as linker flexibility, functionality, and chemical crosslinking on the macroscale mechanical properties of DNA hydrogels. In this review, we present the existing data and methods regarding the mechanical design of pure DNA hydrogels and hybrid DNA hydrogels, and their use as hydrogels for cell culture. The aim of this review is to facilitate further study and development of DNA hydrogels towards utilizing their full potential as multifeatured and highly programmable biomaterials with controlled mechanical properties.
A New Indicator to Assess Public Perception of Air Pollution Based on Complaint Data
Authors: Sun Yong , Jin Fengxiang , Zheng Yan , Ji Min , Wang Huimeng
Published: February 2021

Severe air pollution problems have led to a rise in the Chinese public’s concern, and it is necessary to use monitoring stations to monitor and evaluate pollutant levels. However, monitoring stations are limited, and the public is everywhere. It is also essential to understand the public’s awareness and behavioral response to air pollution. Air pollution complaint data can more directly reflect the public’s real air quality perception than social media data. Therefore, based on air pollution complaint data and sentiment analysis, we proposed a new air pollution perception index (APPI) in this paper. Firstly, we constructed the emotional dictionary for air pollution and used sentiment analysis to calculate public complaints’ emotional intensity. Secondly, we used the piecewise function to obtain the APPI based on the complaint Kernel density and complaint emotion Kriging interpolation, and we further analyzed the change of center of gravity of the APPI. Finally, we used the proposed APPI to examine the 2012 to 2017 air pollution complaint data in Shandong Province, China. The results were verified by the POI (points of interest) data and word cloud analysis. The results show that: (1) the statistical analysis and spatial distribution of air pollution complaint density and public complaint emotion intensity are not entirely consistent. The proposed APPI can more reasonably evaluate the public perception of air pollution. (2) The public perception of air pollution tends to the southwest of Shandong Province, while coastal cities are relatively weak. (3) The content of public complaints about air pollution mainly focuses on the exhaust emissions of enterprises. Moreover, the more enterprises gather in inland cities, the public perception of air pollution is stronger.
A Geometric Algorithm to Evaluate the Thickness Distribution of Stretched Sheets through Finite Element Analysis
Authors: Giuliano Gillo , Corrado Andrea , Polini Wilma
Published: February 2021

Industry 4.0 aims to digitalize the manufacturing process to increase the productivity and the product quality of plants. A fundamental aspect of the digitalized manufacturing processes is the simulation of the manufacturing process in order to develop its virtual representation, known as digital twin, whose purposes may be monitoring, and control. Algorithms to elaborate the simulated data in order to improve the control of the manufacturing process are very important and they need to be developed. Sheet metal forming is a widely used process to manufacture parts with a high production rate and a low cost. The thinning of the stretched sheet needs to be controlled in detail, because it is strongly connected with the product quality. This work presents a simulation model and a geometric algorithm to evaluate the thickness distribution of a sheet stretched through a forming process. In order to accurately evaluate the thickness trend, a geometric algorithm was proposed which, on the basis of the position of the nodes of the internal and external surface of the sheet, was able to evaluate the thickness value. It enables finding of the minimum value of the stretched sheet thickness. The geometric algorithm was slightly modified, in a second step of the work, to experimentally evaluate the thickness trend of a sheet stretched by a forming process; it was applied to the measurement points obtained through a coordinate measurement machine on the inner and outer surfaces of the sheet. The numerical–experimental comparison of the results shows the appropriateness of the proposed algorithm for numerical data.
An Optimal Task Assignment Strategy in Cloud-Fog Computing Environment
Authors: Tsai Jung-Fa , Huang Chun-Hua , Lin Ming-Hua
Published: February 2021

With the advent of the Internet of Things era, more and more emerging applications need to provide real-time interactive services. Although cloud computing has many advantages, the massive expansion of the Internet of Things devices and the explosive growth of data may induce network congestion and add network latency. Cloud-fog computing processes some data locally on edge devices to reduce the network delay. This paper investigates the optimal task assignment strategy by considering the execution time and operating costs in a cloud-fog computing environment. Linear transformation techniques are used to solve the nonlinear mathematical programming model of the task assignment problem in cloud-fog computing systems. The proposed method can determine the globally optimal solution for the task assignment problem based on the requirements of the tasks, the processing speed of nodes, and the resource usage cost of nodes in cloud-fog computing systems.