bluuebunny/crossref_metadata_2025 huggingface dataset:
Cut-Out Animation dalam Perancangan Konten Youtube Bertema Kepahlawanan
Authors: Sutrisno Arif , Rokhman Nur
Published: March 2023

AbstrakYoutube merupakan media yang baik untuk membantu anak-anak belajar tentang sejarah. Namun konten sejarah di kanal Youtube dalam bentuk masih belum banyak ditemui salah satunya karena proses pembuatan konten animasi yang cenderung sulit. Cut-out animation merupakan teknik animasi yang sederhana dalam proses produksinya sehingga penelitian ini bertujuan untuk merancang sebuah film animasi infografis dengan konten sejarah dengan pemilihan kisah Pahlawan Indonesia yaitu pangeran Diponegoro dengan menggunakan teknik cut-out animation untuk konten Youtube. Metode penelitian yang digunakan adalah MDLC (Multimedia Development Life Cycle) meliputi Concept (Topic, Data Search, Analysis, Synopsis, Script), Design (Asset Design, Storyboard), Material Collecting (Dubbing), Assembly (Animate, Input Audio, Editing), Testing (Rendering, Testing), dan Distribution (Publishing). Penelitian ini menghasilkan film animasi sejarah berjudul Diponegoro berdasarkan kisah Pangeran Diponegoro yang dibuat dengan teknik cut-out animation. Teknik cut-out animation sangat cocok untuk konten Youtube khususnya yang bertema kepahlawanan karena proses produksinya sederhana dan hasilnya menarik, singkat, lengkap, sederhana, membantu daya ingat informasi, serta mendorong penonton untuk berlangganan (subscribe). Kata Kunci: Animasi Sejarah, Cut-out animation, MDLC, Youtube AbstractYoutube is an excellent medium to help children learn about history. However, historical content on the Youtube channel in the form still needs to be widely found because the process of creating animated content tends to be complicated. Cut-out animation is a simple animation technique in the production process. This research aims to design an infographic animated film with historical content by choosing the story of an Indonesian hero, Prince Diponegoro, using cut-out animation techniques for YouTube content. The research method used is MDLC (Multimedia Development Life Cycle) covering Concept (Topic, Data Search, Analysis, Synopsis, Script), Design (Asset Design, Storyboard), Material Collecting (Dubbing), Assembly (Animate, Audio Input, Editing), Testing (Rendering, Testing), and Distribution (Publishing). This research produced a historical animated film entitled Diponegoro based on Prince Diponegoro's story, which was made using cut-out animation techniques. The cut-out animation technique is very suitable for Youtube content, especially those with heroic themes because the production process is simple. The results are interesting, short, complete, and simple, which helps information retention and encourages viewers to subscribe. Keyword: Cut-out animation, Historical Animation, MDLC, Youtube
A Curriculum Development Project for a School Nursing Certification Program in Turkey: A Delphi Study
Authors: OZDEMİR İrem Nur , KADIOĞLU Hasibe
Published: March 2023

Objective: School nursing education has not been standardized in Turkey so that it was decided the Delphi technique would be used to develop a curriculum for a program of education in school nursing.

Methods: The data were collected from the expert panel via the Delphi technique by means of e-mail. Data were collected in three consecutive Delphi rounds. The population of the study comprised academic nurses and school nurses working in the area of school nursing in Turkey. A total

of 83 experts were invited to participate in the study. The data were evaluated in terms of Quartile 1, Quartile 3, medians, interquartile ranges. Interquartile range refers to the difference between Quartile 3 and 1. Consensus was considered as less than the interquartile range of 1.2.

Results: At the end of Round-1, 177 learning outcomes, nine main topics, 262 sub-topics were obtained. At the end of Round-2, 167 learning outcomes, nine main and 255 sub-topics were gathered, while at the end of Round-3, agreement was reached regarding nine main topics, 255 sub-topics, 167 learning outcomes, thus ending the Delphi rounds.

Conclusions: The curriculum on which agreement was reached will be treated as a guide to school nursing education and contribute to the standardization of this instruction.
Evaluation of the Effect of Hedonic Hunger on Nutrition Change Processes and Its Relationship with BMI: A Study on University Students
Authors: ARSLAN Müge , YABANCI AYHAN Nurcan , ÇOLAK Hatice , ÇEVİK Ekin , SARIYER Esra Tansu
Published: March 2023

Objective: The aim of this study is to evaluate the effect of hedonic hunger on nutritional change processes and its relationship with BMI in university students.

Methods: A questionnaire consisting of sociodemographic characteristics, questions about eating habits, Power of Food Scale (PFS) and Nutrition Change Processes Scale (NPCS) were applied to 1003 undergraduate students.

Results: Majority of the students were female and normal weight in terms of BMI. The median PFS and score of the obese students is higher than the normal ones. The median NPCS scores of obese students are higher than other BMI classifications (p< .01). The median scores of food available, food present and food taste sub-factors of PFS are statistically higher in obese students than in normal-weight students (p< .01). The sub-factors of NPCS that consciousness raising, dramatic relief, self-reevaluation, social liberation, contingency management, self-liberation, stimulus control median scores are statistically higher in obese students than in normal-weight students. As hedonic hunger increases, the nutritional change process increases by 13.7%. The increase in hedonic hunger affects the nutritional change processes positively by 46.1% (p< .001).

Conclusion: Hedonic hunger and nutrition change processes of obese students are higher than those of normal weight, and as hedonic hunger increases, the process of nutritional change increases, and the increase in hedonic hunger positively affects nutritional change processes.
Pain and Malnutrition in Elderly Cancer Patients: Examples of Southern Turkey
Authors: AYTAÇ Sema , OVAYOLU Özlem , SERÇE Sibel , OVAYOLU Nimet
Published: March 2023

Objective: The prevalence of geriatric cancer disease is gradually increasing. Both the cancer diagnosis and many accompanying symptoms affect individuals bio-psycho-socially and impair the quality of life. This study was conducted to evaluate pain and nutritional status in geriatric cancer patients.

Methods: This descriptive study was conducted with geriatric cancer patients receiving treatment in the ambulatory chemotherapy outpatient clinic of a university and oncology hospital. The population of the study consisted of cancer patients receiving treatment in the hospital and the sample consisted of 215 geriatric cancer patients who were voluntary to participate in the study. The data of the study were collected using a questionnaire, the Visual Analog Scale (VAS), and the Mini Nutritional Assessment.

Results: The mean age of the patients was 69.3±4.7 years. Respiratory tract cancers ranked first (28.8%). 42.8% of the patients were at stage four and they frequently experienced symptoms such as fatigue, loss of appetite and poor nutrition. VAS mean score was 4.8±2.8, Mini nutritional assessment mean score was 9.2±3.0, and there was a negative significant correlation between the VAS and Mini nutritional assessment mean scores (p
PERANCANGAN APLIKASI PERKANTORAN ELEKTRONIK DENGAN MENGGUNAKAN METODE OBJECT ORIENTED ANALYSIS DESIGN BERBASIS WEB PADA KJPP DAR
Authors: Rivai Samuel , Tukino Tukino
Published: March 2023

This project aims to create an electronic office application to effectively handle administrative administration in the department. The results are then used to the OOAD-based design of electronic office applications, which might facilitate management of search data and administration. The requirements phase kicks off the OOAD stages, which then go on to analysis, design, implementation, and testing. Modeling is done using the Unified Modeling Language (UML), which also uses Use Case Diagrams, Activity Diagrams, Class Diagrams, and Sequence Diagrams. The object-oriented analysis design methodology is being used by the IT Department of PT Infineon Batam to produce electronic office applications, and it is based on a website developed using the PHP MySQL database programming language. The results of this study include the creation of an electronic office application using the OOAD methodology, which has been implemented via the localhost webserver. These results include a system analysis, interface system design, testing of the system with a black box system, and testing results with test sample cases that have been carried out to determine that this software can be used correctly.
IMPLEMENTASI METODE FINITE STATE AUTOMATA PADA SCREENING AWAL PENERIMAAN PEGAWAI
Authors: Annisa Novtariany  , Gata Windu , Hermalani Enie Heni , Rahayu Sri , Nofitasari Hafifah Bella
Published: March 2023

Every company always has a recruitment selection process with the hope that the company can get competent employees. The selection process usually has several criteria that are usually predetermined by the company which are needed in the initial selection process for hiring employees. Thus it is necessary for an application system that can carry out the initial screening process for employee recruitment to assist the implementation of the selection process. The design of this application uses the FSA implementation with the NFA type as an abstract machine to determine the stages and transitions contained in these stages which are used to determine the results of the initial screening of employee recruitment. If they meet the specified criteria, they will pass at this stage. This is a role in making abstract machines for the initial screening process for employee recruitment using Nondeterministic Finite Automata (NFA) because each stage can lead to several stages at once.
SISTEM PENDUKUNG KEPUTUSAN PEMILIHAN SUPPLIER TERBAIK MENGGUNAKAN METODE SIMPLE ADDITIVE WEIGHTING
Authors: Felicia Elvira , Badrul Mohammad
Published: March 2023

Keberadaan pemasok atau supplier memegang peranan penting dalam ketersediaan bahan baku untuk berlangsungnya aktivitas produksi suatu perusahaan. Peran supplier sangatlah vital dalam suatu perusahaan, sehingga supplier harus tetap menjaga ketersediaan barang agar aktivitas perusahaan dapat berjalan dengan lancar. PT. Dutamasindo Labora Jaya adalah perusahaan yang bergerak dibidang logistik dan distribusi. Perusahaan dituntut untuk memberikan pelayanan terbaik ke pelanggan supaya kinerja perusahaan tetap stabil dan tentunya diharapkan meningkat seperti yang sudah dicanangkan oleh bagian manajemen. PT. Dutamasindo Labora Jaya dituntut untuk bekerja sama dengan banyak perusahaan supplier untuk memenuhi kebutuhan operasional perusahaan. Masalah yang dihadapi perusahaan adalah sulitnya menentukan supplier mana yang memiliki performasi baik dari segi waktu pengiriman, kualitas dan kuantitas sehingga perusahaan bisa memprioritaskan supplier tersebut dalam memenuhi bahan baku yang dibutuhkan. Pada penelitian ini penulis akan mengimplementasikan sistem pendukung keputusan untuk pemilihan supplier terbaik dengan menggunakan metode simple additive weighting Karena metode ini dapat menentukan nilai bobot dari setiap atribut dan kemudian mengurutkan alternatif terbaik dari beberapa alternatif berupa ranking tertinggi sampai terendah, mudah diimplementasikan, dan menggunakan konsep pembobotan.
PERANCANGAN LEARNING MANGEMENT SYSTEM BERBASIS ANDROID
Authors: Andi Firdaus Muhammad , Husain Ahmad , Dwi Puspitasari Anggun Citra Dini
Published: March 2023

Tujuan dari penelitian ini adalah untuk merancang aplikasi pendamping kegiatan pembelajaran, sebelumnya SMPN 163 Jakarta menggunakan aplikasi pihak ketiga untuk menemani kegiatan pembelajaran. Selain itu, penelitian ini juga bertujuan untuk membuat aplikasi pendamping belajar yang terintegrasi dan terintegrasi untuk SMPN 163 Jakarta untuk mengatasi masalah inkonsistensi penggunaan aplikasi oleh setiap guru di SMPN 163 Jakarta. Waktu penelitian dilakukan dari bulan Maret sampai Juli 2022. Penelitian ini menggunakan pendekatan kualitatif dengan mengembangkan sistem menggunakan metode research and development. Hasil dari penelitian ini adalah aplikasi yang peneliti buat dapat mengintegrasikan kegiatan pembelajaran dari aplikasi pihak ketiga. Dengan adanya aplikasi yang peneliti buat, maka aplikasi yang digunakan sebagai pendamping belajar oleh guru SMPN 163 Jakarta dapat diganti dengan aplikasi yang peneliti buat, dan mengatasi masalah ketidakkonsistenan penggunaan aplikasi pendamping belajar. Selain itu dengan adanya aplikasi yang peneliti buat dapat menyimpan data administrasi untuk kegiatan belajar yang lebih aman karena tidak lagi menggunakan media kertas sehingga tidak ada resiko kertas hilang atau robek.
SISTEM PAKAR DIAGNOSA PENYAKIT SAPI SEBAGAI UPAYA PENCEGAHAN PENYEBARAN WABAH PMK DI LAMONGAN
Authors: Zamroni Moh. Rosidi , Cita K. N. S Qabilah , Wahyudi Agung
Published: March 2023

On May 5, 2022, Indonesia was hit by an outbreak of PMK in livestock. Until now, in August 2022, there are 20 provinces, 215 districts/cities, 1,808 sub-districts and 7,434 villages that are still actively infected with nail and mouth disease (PMK). The emergence of PMK is an epidemic that results in huge losses to cattle farmers. Especially if you do not have knowledge about the transmission of the disease. One of the efforts to prevent the spread of livestock disease is to implement an expert system for diagnosing cattle disease using the forward chaining method. Where this system will help farmers to provide knowledge of disease symptoms and diagnosis of disease, so that farmers are able to take initial action to handle their livestock.
PERBANDINGAN ALGORITMA NAÏVE BAYES CLASSIFIER DAN K-NEAREST NEIGHBORS UNTUK ANALISIS SENTIMEN COVID-19 DI TWITTER
Authors: Habibi Habibi Aulia Nur Syifa , Nugroho Arie , Firliana Rina
Published: March 2023

COVID-19 muncul di Tiongkok pada tahun 2019. Di Indonesia pada tahun 2020 terdapat lebih dari 3000 kasus positif COVID-19 dengan angka kematian sebesar 9,1%. Belum tuntasnya upaya pemerintah dalam memutus mata rantai penyebaran COVID-19 membuat masyarakat resah akan pandemi ini. Banyak masyarakat yang ingin menyampaikan aspirasinya di media sosial yang dianggap cocok sebagai wadah yang mewakili aspirasi pandemi COVID-19. Salah satunya adalah twitter. Banyak sekali pesan teks yang dikirimkan, ada yang positif dan ada yang negatif, sehingga sulit untuk mendapatkan informasi yang selaras karena keberagaman pesan teks yang dikirimkan. Salah satu cara untuk mengatasinya adalah dengan analisis sentimen. Penelitian ini memiliki proses antara lain text preprocessing, pembobotan kata, klasifikasi dengan algoritma K-Nearest Neighbors (KNN) dan Naïve Bayes Classifiers (NBC). Hasil yang didapatkan KNN mendapatkan akurasi sebesar 72.37% sedangkan NBC sebesar 67.84%. KNN merupakan algoritma klasifikasi terbaik untuk klasifikasi sentimen negatif, label negatif yang diprediksi benar pada KNN lebih besar yaitu 393 dibandingkan dengan NBC yaitu 339. Sedangkan NBC merupakan algoritma terbaik untuk klasifikasi sentimen positif, label positif yang diprediksi benar NBC yaitu 275 lebih besar dibandingkan dengan KNN yaitu 262.
SISTEM INFORMASI KETERSEDIAAN DAN PERKEMBANGAN HARGA BAHAN POKOK SECARA REAL TIME BERBASIS WEB
Authors: Sufri Rahmat , Mukhroji Mukhroji , Nazuarsyah Nazuarsyah , Ginting Rossiana Br , Prayudi Satria
Published: March 2023

The Office of Industry, Trade, Cooperatives, Small and Medium Enterprises of Pidie Regency has not yet used a media website that can provide information on the availability and price developments of basic commodities. Data on the development of prices for basic goods is very important for the agency overseeing the trade sector. This is also very useful for identifying the causes of price fluctuations or fluctuations in an effort to maintain the stability of price developments. So we need a system that records information on the availability and price developments of staple goods in real time, the design of this system uses the PHP programming language and MySQL Server Database which allows it to be accessed anywhere and anytime. The benefit to be gained from this system is that it makes it easier for the Office of Industry, Trade, Cooperatives, Small and Medium Enterprises of the Pidie Regency to convey information on prices of basic goods to the public quickly and accurately
Silk and its composites for humidity and gas sensing applications
Authors: Jain Shubhanth , Vedavyas V. , Prajwal R. V. , Shaji Malavika , Nath Vishnu G , Angappane S. , Achutharao Govindaraj
Published: March 2023

Silk fibroin (SF) is a natural protein largely used in the textile industry with applications in bio-medicine, catalysis as well as in sensing materials. SF is a fiber material which is bio-compatible, biodegradable, and possesses high tensile strength. The incorporation of nanosized particles into SF allows the development of a variety of composites with tailored properties and functions. Silk and its composites are being explored for a wide range of sensing applications like strain, proximity, humidity, glucose, pH and hazardous/toxic gases. Most studies aim at improving the mechanical strength of SF by preparing hybrids with metal-based nanoparticles, polymers and 2D materials. Studies have been conducted by introducing semiconducting metal oxides into SF to tailor its properties like conductivity for use as a gas sensing material, where SF acts as a conductive path as well as a substrate for the incorporated nanoparticles. We have reviewed gas and humidity sensing properties of silk, silk with 0D (i.e., metal oxide), 2D (e.g., graphene, MXenes) composites. The nanostructured metal oxides are generally used in sensing applications, which use its semiconducting properties to show variation in the measured properties (e.g., resistivity, impedance) due to analyte gas adsorption on its surface. For example, vanadium oxides (i.e., V2O5) have been shown as candidates for sensing nitrogen containing gases and doped vanadium oxides for sensing CO gas. In this review article we provide latest and important results in the gas and humidity sensing of SF and its composites.
Understanding the multi-sign communication system: A study on Peking Opera stage translation
Authors: Zhang Jiadong , Zou Deyan
Published: March 2023

Peking Opera is an artist-centered art rather than a director-centered one, and its true significance lies in its stage performance. However, the realization of the meaning of the “Performance Text” is the result of the joint action of different sign systems. Therefore, translators engaged in the stage translation of Peking Opera must understand the meaning and operation mechanism of this multi-sign system. From the perspective of semiotics of theater and drama, this paper deconstructs the Peking Opera sign system and divides them into several subsystems, describes in detail the characteristics of each secondary system, especially their semiotization process, their manners of meaning transmission, the rules of interaction among them, and finally presents the meaning generation and communication mechanism of this “Performance Text”. On this basis, the paper clarifies that the main tasks and challenges faced by translators engaged in Peking Opera stage translation which are they should not only translate the language but also need to find time and space to realize the transformation of the meaning of the non-verbal sign system.
Geotechnical aspects of alluvial soils at different depths under sodium chloride action in Najran region, Saudi Arabia: Field supported by laboratory tests
Authors: El Aal Ahmed Abd , Abdullah Gamil M. S. , Zakaly Hesham M. H. , Awad Hamdy A. , Omar Ali E. , Sakr Mohamed A. H. , Ene Antoaneta
Published: March 2023

The current research study is aimed at studying the impact of sodium chloride on the performance of semi-arid soils in the Najran area of Saudi Arabia. Experimental work has been undertaken to investigate how adding salt to the semi-arid soil collected in the Najran area affects the boundaries of Atterberg, compaction characteristics, California bearing ratio, and shear strength. All testing was conducted on soil samples from different zones of the Najran area at varying depths of 1.5, 3, and 4.5 m along the soil profiles. The soil samples were analyzed individually and then compared with the same soil samples mixed with NaCl at different percentages of 5, 10, and 20% by weight of the dry soil. Using advanced techniques, such as the scanning electron microscope, energy dispersive x-ray analysis, and X-ray diffraction analysis, the stabilization process was examined. The findings revealed that NaCl significantly impacts the geotechnical characteristics of semi-arid soils. The maximum dry density increased from 1.995, 1.93, and 1.96 to 2.02, 1.99, and 2.03 g/cm3, and the optimal water content decreased from 9.47, 13.7, and 11.29 to 7.01, 9.58%, and 8.09% with 20% NaCl added at various depths, respectively. Shear resistance parameters were improved by adding 20% NaCl, where the soil cohesion increased from 0.1333, 0.0872, and 0.0533 to 0.1843, 0.1034, and 0.0372 kg/cm2, and the angle of internal friction increased from 24°, 25.5°, and 29° to 27.8°, 30°, and 33°, respectively. The liquid and plastic limits and, in turn, the plasticity index reduced as the added percentage of NaCl increased. Furthermore, the California bearing ratio percentages significantly increased and reached more than 50%. As a result, it is established that NaCl is an excellent stabilizer, especially at 20% concentration, and might be used as a sub-base substance in highway construction.
Parthenocarpy-related genes induced by naphthalene acetic acid in oil palm interspecific O × G [Elaeis oleifera (Kunth) Cortés × Elaeis guineensis Jacq.] hybrids
Authors: Montoya Carmenza , Mejia-Alvarado Fernan Santiago , Botero-Rozo David , Ayala-Diaz Ivan Mauricio , Romero Hernan Mauricio
Published: March 2023

Parthenocarpy is the development without fertilization of seedless fruits. In the oil palm industry, the development of parthenocarpic fruits is considered an attractive option to increase palm oil production. Previous studies have shown the application of synthetic auxins in Elaeis guineensis, and interspecific O×G hybrids (Elaeis oleifera (Kunth) Cortés × E. guineensis Jacq.) induces parthenocarpy. The aim of this study was to identify the molecular mechanism through transcriptomics and biology system approach to responding to how the application of NAA induces parthenocarpic fruits in oil palm O×G hybrids. The transcriptome changes were studied in three phenological stages (PS) of the inflorescences: i) PS 603, pre-anthesis III, ii) PS 607, anthesis, and iii) PS 700, fertilized female flower. Each PS was treated with NAA, Pollen, and control (any application). The expression profile was studied at three separate times: five minutes (T0), 24 hours (T1), and 48 h post-treatment (T2). The RNA sequencing (RNA seq) approach was used with 27 oil palm O×G hybrids for a total of 81 raw samples. RNA-Seq showed around 445,920 genes. Numerous differentially expressed genes (DEGs) were involved in pollination, flowering, seed development, hormone biosynthesis, and signal transduction. The expression of the most relevant transcription factors (TF) families was variable and dependent on the stage and time post-treatment. In general, NAA treatment expressed differentially more genes than Pollen. Indeed, the gene co-expression network of Pollen was built with fewer nodes than the NAA treatment. The transcriptional profiles of Auxin-responsive protein and Gibberellin-regulated genes involved in parthenocarpy phenomena agreed with those previously reported in other species. The expression of 13 DEGs was validated by RT-qPCR analysis. This detailed knowledge about the molecular mechanisms involved in parthenocarpy could be used to facilitate the future development of genome editing techniques that enable the production of parthenocarpic O×G hybrid cultivars without growth regulator application.
Genome-wide analysis for the melatonin trait associated genes and SNPs in dairy goat (Capra hircus) as the molecular breeding markers
Authors: Wu Hao , Yi Qi , Ma Wenkui , Yan Laiqing , Guan Shengyu , Wang Likai , Yang Guang , Tan Xinxing , Ji Pengyun , Liu Guoshi
Published: March 2023

Previous studies have reported that the endogenous melatonin level is positively associated with the quality and yield of milk of cows. In the current study, a total of 34,921 SNPs involving 1,177 genes were identified in dairy goats by using the whole genome resequencing bulked segregant analysis (BSA) analysis. These SNPs have been used to match the melatonin levels of the dairy goats. Among them, 3 SNPs has been identified to significantly correlate with melatonin levels. These 3 SNPs include CC genotype 147316, GG genotype 147379 and CC genotype 1389193 which all locate in the exon regions of ASMT and MT2 genes. Dairy goats with these SNPs have approximately 5-fold-higher melatonin levels in milk and serum than the average melatonin level detected in the current goat population. If the melatonin level impacts the milk production in goats as in cows, the results strongly suggest that these 3 SNPs can serve as the molecular markers to select the goats having the improved milk quality and yield. This is a goal of our future study.
Architecture of the SARS-CoV-2-specific T cell repertoire
Authors: Zornikova Ksenia V. , Sheetikov Saveliy A. , Rusinov Alexander Yu , Iskhakov Rustam N. , Bogolyubova Apollinariya V.
Published: March 2023

The T cell response plays an indispensable role in the early control and successful clearance of SARS-CoV-2 infection. However, several important questions remain about the role of cellular immunity in COVID-19, including the shape and composition of disease-specific T cell repertoires across convalescent patients and vaccinated individuals, and how pre-existing T cell responses to other pathogens—in particular, common cold coronaviruses—impact susceptibility to SARS-CoV-2 infection and the subsequent course of disease. This review focuses on how the repertoire of T cell receptors (TCR) is shaped by natural infection and vaccination over time. We also summarize current knowledge regarding cross-reactive T cell responses and their protective role, and examine the implications of TCR repertoire diversity and cross-reactivity with regard to the design of vaccines that confer broader protection against SARS-CoV-2 variants.
Hormonal and inflammatory modulatory effects of hesperidin in hyperthyroidism-modeled rats
Authors: Ashry Mahmoud , Askar Hussam , Obiedallah Manar M. , Elankily Ahmed Hussuin , Galal El-Sahra Doaa , Zayed Gamal , Mustafa Mohamed A. , El-Shamy Sawsan Abd El-Maksoud , Negm Somaia A. , El-Beltagy Marwa A. , Abdel-Wahhab Khaled G. , Ene Antoaneta
Published: March 2023

The goal of the current study was to investigate the hormonal modulatory efficiency of hesperidin, through its regulatory potential of immunological, inflammatory, and/or antioxidant changes in on hyperthyroidism modeled adult female albino rats. Both normal and hyperthyroidism modeled rats (140-160g) were randomly divided into four groups (10 animals each) as follows: 1) healthy animals were daily ingested with saline for six weeks, and served as control group, 2) healthy animals were intraperitoneally injected with hesperidin (50 mg/kg/day) for a similar period, 3) hyperthyroidism-modeled animals without any treatment acted as positive control, and 4) hyperthyroidism-modeled animals were treated intraperitoneally with hesperidin for a similar period. The findings showed that hesperidin significantly modulated hyperthyroidism deteriorations, this was evidenced by a remarkable decline in serum T4, FT4, T3, FT3, TNF-α, IL1β-, IL4-, IL-6, and IL-10 levels, with a minor increase in TSH and significant raise in CD4+ level. Similarly, valuable improvement was observed in the oxidative status; serum SOD, GPx, CAT, and GSH levels were dramatically enhanced, associated with remarkable drop in MDA and NO levels. Also, hesperidin demonstrated nephro-hepatoprotective and anti-atherogenic potential, this was achieved from the notable reduction in ALAT and ASAT activities as well as urea, creatinine, cholesterol, and triglyceride close to the corresponding values of healthy group. These findings were supported by histological and immunohistochemical ones that showed a notable decrease in the expression of the calcitonin antibody. In conclusion, hesperidin possesses anti-hyperthyroidism, immunoinflammatory regulatory, and antioxidant activities that evidenced from the improvement of physio-architecture of the thyroid gland, reduction of inflammation and restoration of the impaired oxidative stress. This effect might be mechanized through immunological, inflammatory, apoptotic, and/or antioxidant modulatory pathways.
Identification of immunotherapy and chemotherapy-related molecular subtypes in colon cancer by integrated multi-omics data analysis
Authors: Zhu Jie , Kong Weikaixin , Huang Liting , Bi Suzhen , Jiao Xuelong , Zhu Sujie
Published: March 2023

Background

Colon cancer is a highly heterogeneous disease, and identifying molecular subtypes can provide insights into deregulated pathways within tumor subsets, which may lead to personalized treatment options. However, most prognostic models are based on single-pathway genes.

Methods

In this study, we aimed to identify three clinically relevant subtypes of colon cancer based on multiple signaling pathways-related genes. Integrative multi-omics analysis was used to explain the biological processes contributing to colon cancer aggressiveness, recurrence, and progression. Machine learning methods were employed to identify the subtypes and provide medication guidance for distinct subtypes using the L1000 platform. We developed a robust prognostic model (MKPC score) based on gene pairs and validated it in one internal test set and three external test sets. Risk-related genes were extracted and verified by qPCR.

Results

Three clinically relevant subtypes of colon cancer were identified based on multiple signaling pathways-related genes, which had significantly different survival state (Log-Rank test, p<0.05). Integrative multi-omics analysis revealed biological processes contributing to colon cancer aggressiveness, recurrence, and progression. The developed MKPC score, based on gene pairs, was robust in predicting prognosis state (Log-Rank test, p<0.05), and risk-related genes were successfully verified by qPCR (t test, p<0.05). An easy-to-use web tool was created for risk scoring and therapy stratification in colon cancer patients, and the practical nomogram can be extended to other cancer types.

Conclusion

In conclusion, our study identified three clinically relevant subtypes of colon cancer and developed a robust prognostic model based on gene pairs. The developed web tool is a valuable resource for researchers and clinicians in risk scoring and therapy stratification in colon cancer patients, and the practical nomogram can be extended to other cancer types.
Influence of the Caribbean Sea eddy field on Loop Current predictions
Authors: Le Hénaff Matthieu , Kourafalou Vassiliki H. , Androulidakis Yannis , Ntaganou Nektaria , Kang HeeSook
Published: March 2023

Previous studies have shown how the passage of eddies from the Caribbean Sea (CS) to the Gulf of Mexico (GoM) can impact the Loop Current (LC) system, in particular the detachments of LC Eddies (LCEs). Here we used numerical modeling to investigate the impact of the eddy field in the CS on LC predictions. We used a HYCOM ocean model configuration of the North Atlantic at 1/12° resolution to perform two data-assimilative experiments: one in which all available observations were assimilated (Ref), and one in which all available observations were assimilated except in the CS, where climatological altimetry values were assimilated instead of actual observations, leading to dampening the mesoscale activity there (NoCarib). These experiments took place in 2015, when the LC was very active with several LCE detachments, re-attachments, and separations. Each of these experiments was used to initialize 28 60-day forecast simulations every 10 days. In terms of model Sea Surface Height (SSH), the forecasts initialized with the Ref experiment had, on average, lower errors than the forecasts initialized with the NoCarib experiment in the southeastern part of the GoM, with a peak during the 31-40 day forecast period. More importantly, the errors in predicting the date of the next LCE detachment or separation were smaller in the forecasts initialized from the more realistic Ref experiment. Finally, the forecasts initialized by the NoCarib experiment showed a much higher level of false negatives predictions, meaning that no LCE detachment was predicted whereas a detachment actually happened. Overall, 68% of LCE detachments were predicted with an error smaller than 15 days in the forecasts initialized from the more realistic Ref experiment, but only 32% in the forecasts initialized from the NoCarib experiment, stressing the importance of the CS eddy field for predicting the LC evolution. These findings have implications on the GoM predictability, highlighting the need to either run data-assimilative models covering both the GoM and the CS, or pay particular attention to accurate boundary conditions for limited-area GoM models.
Uncovering the role of superplasticizer in developing nano-engineered ultra-high-performance concrete
Authors: Wang Lili , Ma Yitong , Li Liangwei
Published: March 2023

The effect of superplasticizer (SP) on the performance of ultra-high-performance concrete (UHPC) and ultra-high-performance fiber-reinforced concrete (UHPFRC) has been systematically investigated aiming to optimize the use of SP. The slump flow, and V-funnel time were employed to evaluate the impact of SP on the workability, while compressive strength had been used for mechanical property. Moreover, the packing density, as well as the water film thickness had been calculated to uncover the mechanism. The obtained results indicated that the addition of SP improved the workability of specimens with an ultimate-low water-to-binder (W/B) ratio, while it benefited the strength development of UHPC with a lower W/B ratio. This novel phenomenon (SP enhances the mechanical properties of UHPC) is due to the fact that SP reduced the water film thickness and enhanced the packing structure, therefore resulting in an increased compressive strength. For UHPFRC, similar trends can be witnessed regarding the flowability. However, the alternation of the fresh behavior of UHPFRC, attributed to the inclusion of SP, had an obvious impact on the fiber distribution, which altered the strength development of UHPFRC. This study revealed the significant effect of SP on the performance, especially on the strength development, of UHPC and UHPFRC.
Diagnostic value of upper airway morphological data based on CT volume scanning combined with clinical indexes in children with obstructive sleep apnea syndrome
Authors: Shi Yanmin , Gu Meilan , Zhang Xin , Wen Mengmeng , Li Runhua , Wang Yifei , Li Chen , Wang Xianli , Yang Ruiyun , Xiao Xinguang
Published: March 2023

Background and purpose

Early diagnosis is important for treatment and prognosis of obstructive sleep apnea (OSA)in children. Polysomnography (PSG) is the gold standard for the diagnosis of OSA. However, due to various reasons, such as inconvenient implementation, less equipped in primary medical institutions, etc., it is less used in children, especially in young children. This study aims to establish a new diagnostic method with imaging data of upper airway and clinical signs and symptoms.

Methods

In this retrospective study, clinical and imaging data were collected from children ≤10 years old who underwent nasopharynx CT scan(low-dose protocol)from February 2019 to June 2020,including 25 children with OSA and 105 non-OSA. The information of the upper airway (A-line; N-line; nasal gap; upper airway volume; upper and lower diameter, left and right diameter and cross-sectional area of the narrowest part of the upper airway) were measured in transaxial, coronal, and sagittal images. The diagnosis of OSA and adenoid size were given according to the guidelines and consensus of imaging experts. The information of clinical signs, symptoms, and others were obtained from medical records. According to the weight of each index on OSA, the indexes with statistical significance were screened out, then were scored and summed up. ROC analysis was performed with the sum as the test variable and OSA as the status variable to evaluate the diagnostic efficacy on OSA.

Results

The AUC of the summed scores (ANMAH score) of upper airway morphology and clinical index for the diagnosis of OSA was 0.984 (95% CI 0.964–1.000). When sum = 7 was used as the threshold (participants with sum>7 were considered to have OSA), the Youden’s index reached its maximum at which point the sensitivity was 88.0%, the specificity was 98.1%, and the accuracy was 96.2%.

Conclusion

The morphological data of the upper airway based on CT volume scan images combined with clinical indices have high diagnostic value for OSA in children; CT volume scanning plays a great guiding role in the selection of treatment scheme of OSA. It is a convenient, accurate and informative diagnostic method with a great help to improving prognosis.

Highlights

– Early diagnosis of OSA in children is very important for the treatment.

– However, the traditional diagnostic gold-standard PSG is difficult to implement.

– This study aims to explore convenient and reliable diagnostic methods for children.

– A new diagnostic model was established combining CT with signs and symptoms.

– The diagnostic method in this study is highly effective, informative, and convenient.
Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut
Authors: Algieri Francesca , Tanaskovic Nina , Rincon Cindy Cardenas , Notario Elisabetta , Braga Daniele , Pesole Graziano , Rusconi Roberto , Penna Giuseppe , Rescigno Maria
Published: March 2023

The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by several players, including the gut microbiota. Various molecules, called postbiotics, are released during the natural metabolic activity of the microbiota. Postbiotics can regulate host–microbe interactions, epithelial homeostasis, and have overall benefits for our health. In this work, we used in vitro and in vivo systems to demonstrate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic (LP-PBF) in preserving intestinal barrier integrity. We demonstrated in vitro that LP-PBF restored the morphology of tight junctions (TJs) that were altered upon Salmonella typhimurium exposure. In vivo, LP-PBF protected the gut vascular barrier and blocked S. typhimurium dissemination into the bloodstream. Interestingly, we found that LP-PBF interacts not only with the host cells, but also directly with S. typhimurium blocking its biofilm formation, partially due to the presence of biosurfactants. This study highlights that LP-PBF is beneficial in maintaining gut homeostasis due to the synergistic effect of its different components. These results suggest that LP-PBF could be utilized in managing several pathologies displaying an impaired intestinal barrier function.
Symptoms of depression, perceived social support, and medical coping modes among middle-aged and elderly patients with type 2 diabetes
Authors: Zhang Chuanyan , Wu Zezhen , Lopez Elna , Magboo Romain G. , Hou Kaijian
Published: March 2023

Objective: To understand the prevalence of depression in diabetes population, explore the relationship between diabetes and depression, and the impact of comprehensive psychological and behavioral intervention on depression related to diabetes and glucose metabolism.

Methods: 71 middle-aged and elderly patients with type 2 diabetes were investigated and evaluated with Self Rating Depression Scale (SDS), Medical Coping Scale (MCWQ) and Social Support Scale (PSSS). Patients who met the research criteria were randomly divided into an experimental group and a control group. The number of effective cases in the two groups was 36 and 35 respectively. In addition to conventional diabetes drug treatment, the experimental group was supplemented with comprehensive psychological and behavioral intervention, while the control group was only given conventional treatment. The fasting blood glucose, 2-h postprandial blood glucose, body weight and depression index were measured before and after treatment in the two groups.

Results: The prevalence of depression in patients with diabetes was as high as 60%, and that in the elderly control group was 5%; In type 2 diabetes population, depression is negatively related to the total score of social support and medical coping surface, and positively related to avoidance, blood sugar, women, course of disease, education level below junior high school, body mass index, and number of complications in medical coping; The fasting blood glucose, 2-h postprandial blood glucose, body mass index, and depression index of the two groups decreased, and the range and speed of decline in the experimental group were higher than those in the control group; There were significant differences between the two groups in fasting blood glucose, 2-h postprandial blood glucose and depression index; During the follow-up period, the blood glucose and depression index of the experimental group increased.

Conclusion: Depression has a high prevalence rate in middle-aged and elderly people with type 2 diabetes, and has a negative impact on blood sugar control in diabetes patients; Psychological and behavioral comprehensive intervention can improve the glucose metabolism and depressive symptoms of middle-aged and elderly patients with type 2 diabetes.
Cardiac autonomic control in Rett syndrome: Insights from heart rate variability analysis
Authors: Cordani Ramona , Tobaldini Eleonora , Rodrigues Gabriel Dias , Giambersio Donatella , Veneruso Marco , Chiarella Lorenzo , Disma Nicola , De Grandis Elisa , Toschi-Dias Edgar , Furlan Ludovico , Carandina Angelica , Prato Giulia , Nobili Lino , Montano Nicola
Published: March 2023

Rett syndrome (RTT) is a rare and severe neurological disorder mainly affecting females, usually linked to methyl-CpG-binding protein 2 (MECP2) gene mutations. Manifestations of RTT typically include loss of purposeful hand skills, gait and motor abnormalities, loss of spoken language, stereotypic hand movements, epilepsy, and autonomic dysfunction. Patients with RTT have a higher incidence of sudden death than the general population. Literature data indicate an uncoupling between measures of breathing and heart rate control that could offer insight into the mechanisms that lead to greater vulnerability to sudden death. Understanding the neural mechanisms of autonomic dysfunction and its correlation with sudden death is essential for patient care. Experimental evidence for increased sympathetic or reduced vagal modulation to the heart has spurred efforts to develop quantitative markers of cardiac autonomic profile. Heart rate variability (HRV) has emerged as a valuable non-invasive test to estimate the modulation of sympathetic and parasympathetic branches of the autonomic nervous system (ANS) to the heart. This review aims to provide an overview of the current knowledge on autonomic dysfunction and, in particular, to assess whether HRV parameters can help unravel patterns of cardiac autonomic dysregulation in patients with RTT. Literature data show reduced global HRV (total spectral power and R-R mean) and a shifted sympatho-vagal balance toward sympathetic predominance and vagal withdrawal in patients with RTT compared to controls. In addition, correlations between HRV and genotype and phenotype features or neurochemical changes were investigated. The data reported in this review suggest an important impairment in sympatho-vagal balance, supporting possible future research scenarios, targeting ANS.
Bibliometric analysis of electroencephalogram research in mild cognitive impairment from 2005 to 2022
Authors: Liu Mingrui , Liu Baohu , Ye Zelin , Wu Dongyu
Published: March 2023

Background

Electroencephalogram (EEG), one of the most commonly used non-invasive neurophysiological examination techniques, advanced rapidly between 2005 and 2022, particularly when it was used for the diagnosis and prognosis of mild cognitive impairment (MCI). This study used a bibliometric approach to synthesize the knowledge structure and cutting-edge hotspots of EEG application in the MCI.

Methods

Related publications in the Web of Science Core Collection (WosCC) were retrieved from inception to 30 September 2022. CiteSpace, VOSviewer, and HistCite software were employed to perform bibliographic and visualization analyses.

Results

Between 2005 and 2022, 2,905 studies related to the application of EEG in MCI were investigated. The United States had the highest number of publications and was at the top of the list of international collaborations. In terms of total number of articles, IRCCS San Raffaele Pisana ranked first among institutions. The Clinical Neurophysiology published the greatest number of articles. The author with the highest citations was Babiloni C. In descending order of frequency, keywords with the highest frequency were “EEG,” “mild cognitive impairment,” and “Alzheimer’s disease”.

Conclusion

The application of EEG in MCI was investigated using bibliographic analysis. The research emphasis has shifted from examining local brain lesions with EEG to neural network mechanisms. The paradigm of big data and intelligent analysis is becoming more relevant in EEG analytical methods. The use of EEG to link MCI to other related neurological disorders, and to evaluate new targets for diagnosis and treatment, has become a new research trend. The above-mentioned findings have implications in the future research on the application of EEG in MCI.
Effects of different physical activity interventions on children with attention-deficit/hyperactivity disorder: A network meta-analysis of randomized controlled trials
Authors: Li Dong , Wang Deng , Cui Wenlai , Yan Jin , Zang Wanli , Li Chenmu
Published: March 2023

Background

Previous studies have shown that physical activity interventions positively affect core symptoms and executive functioning in children with attention-deficit/hyperactivity disorder (ADHD). However, comparisons between different physical activity interventions still need to be made. This study is the first to analyze the effects of 10 different types of physical activity on children with ADHD through a network meta-analysis.

Methods

PubMed, Embase, Web of Science, and Cochrane Library databases were searched for randomized controlled trials on the effects of physical activity interventions on children with ADHD. The search time frame was from database creation to October 2022. Two investigators independently performed literature screening, extraction, and quality assessment. Network meta-analysis was performed with Stata 15.1.

Results

A total of 31 studies were included, and the results indicated that perceptual-motor training was the most effective in terms of motor ability and working memory (SUCRA = 82.7 and 73.3%, respectively). For attention problems and cognitive flexibility, aquatic exercise was the most effective (SUCRA = 80.9 and 86.6%, respectively). For social problems, horsemanship was the most effective (SUCRA = 79.4%). For inhibition switching, cognitive-motor training was the most effective (SUCRA = 83.5%).

Conclusion

Our study revealed that aquatic exercise and perceptual-motor training had a superior overall performance. However, the effects of various physical activity interventions on different indicators in children with ADHD can vary depending on the individual and the intervention’s validity. To ensure an appropriate physical activity intervention is selected, it is important to assess the severity of symptoms exhibited by children with ADHD beforehand.