bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Direct Yaw-Moment Control via Neural-Network-Based Non-Singular Fast Terminal Sliding Mode Control for Electric Vehicles
Authors: Lee Jung Eun , Kim Byeong Woo
Published: June 2024

Given the increased significance of electric vehicles in recent years, this study aimed to develop a novel form of direct yaw-moment control (DYC) to enhance the driving stability of four-wheel independent drive (4WID) electric vehicles. Specifically, this study developed an innovative non-singular fast terminal sliding mode control (NFTSMC) method that integrates NFTSM and a fast-reaching control law. Moreover, this study employed a radial basis function neural network (RBFNN) to approximate both the entire system model and uncertain components, thereby reducing the computational load associated with a complex system model and augmenting the overall control performance. Using the aforementioned factors, the optimal additional yaw moment to ensure the lateral stability of a vehicle is determined. To generate the additional yaw moment, we introduce a real-time optimal torque distribution method based on the vertical load ratio. The stability of the proposed approach is comprehensively verified using the Lyapunov theory. Lastly, the validity of the proposed DYC system is confirmed by simulation tests involving step and sinusoidal inputs conducted using Matlab/Simulink and CarSim software. Compared to conventional sliding mode control (SMC) and NFTSMC methods, the proposed approach showed improvements in yaw rate tracking accuracy for all scenarios, along with a significant reduction in the chattering phenomenon in control torques.
Wearable Robot Design Optimization Using Closed-Form Human–Robot Dynamic Interaction Model
Authors: Shahabpoor Erfan , Gray Bethany , Plummer Andrew
Published: June 2024

Wearable robots are emerging as a viable and effective solution for assisting and enabling people who suffer from balance and mobility disorders. Virtual prototyping is a powerful tool to design robots, preventing the costly iterative physical prototyping and testing. Design of wearable robots through modelling, however, often involves computationally expensive and error-prone multi-body simulations wrapped in an optimization framework to simulate human–robot–environment interactions. This paper proposes a framework to make the human–robot link segment system statically determinate, allowing for the closed-form inverse dynamics formulation of the link–segment model to be solved directly in order to simulate human–robot dynamic interactions. The paper also uses a technique developed by the authors to estimate the walking ground reactions from reference kinematic data, avoiding the need to measure them. The proposed framework is (a) computationally efficient and (b) transparent and easy to interpret, and (c) eliminates the need for optimization, detailed musculoskeletal modelling and measuring ground reaction forces for normal walking simulations. It is used to optimise the position of hip and ankle joints and the actuator torque–velocity requirements for a seven segments of a lower-limb wearable robot that is attached to the user at the shoes and pelvis. Gait measurements were carried out on six healthy subjects, and the data were used for design optimization and validation. The new technique promises to offer a significant advance in the way in which wearable robots can be designed.
SLGA-YOLO: A Lightweight Castings Surface Defect Detection Method Based on Fusion-Enhanced Attention Mechanism and Self-Architecture
Authors: Wang Chengjun , Wang Yifan
Published: June 2024

Castings’ surface-defect detection is a crucial machine vision-based automation technology. This paper proposes a fusion-enhanced attention mechanism and efficient self-architecture lightweight YOLO (SLGA-YOLO) to overcome the existing target detection algorithms’ poor computational efficiency and low defect-detection accuracy. We used the SlimNeck module to improve the neck module and reduce redundant information interference. The integration of simplified attention module (SimAM) and Large Separable Kernel Attention (LSKA) fusion strengthens the attention mechanism, improving the detection performance, while significantly reducing computational complexity and memory usage. To enhance the generalization ability of the model’s feature extraction, we replaced part of the basic convolutional blocks with the self-designed GhostConvML (GCML) module, based on the addition of p2 detection. We also constructed the Alpha-EIoU loss function to accelerate model convergence. The experimental results demonstrate that the enhanced algorithm increases the average detection accuracy (mAP@0.5) by 3% and the average detection accuracy (mAP@0.5:0.95) by 1.6% in the castings’ surface defects dataset.
Dynamic Simulation and Modeling of a Novel NeuRaiSya for Railway Monitoring System Using Petri Nets
Authors: Deplomo Bhai Nhuraisha I. , Villaverde Jocelyn F. , Paglinawan Arnold C.
Published: June 2024

This research introduces the NeuRaiSya (Neural Railway System Application), an innovative railway signaling system integrating deep learning for passenger analysis. The objectives of this research are to simulate the NeuRaiSya and evaluate its effectiveness using the GreatSPN tool (graphical editor for Petri nets). GreatSPN facilitates evaluations of system behavior, ensuring safety and efficiency. Five models were designed and simulated using the Petri nets model, including the Dynamics of Train Departure model, Train Operations with Passenger Counting model, Timestamp Data Collection model, Train Speed and Location model, and Train Related-Issues model. Through simulations and modeling using Petri nets, the study demonstrates the feasibility of the proposed NeuRaiSya system. The results highlight its potential in enhancing railway operations, ensuring passenger safety, and maintaining service quality amidst the evolving railway landscape in the Philippines.
A Low-Power High-Sensitivity Photocurrent Sensory Circuit with Capacitive Feedback Transimpedance for Photoplethysmography Sensing
Authors: Mohan Neethu , Awwad Falah , Albastaki Nabil , Atef Mohamed
Published: June 2024

This study presents an integrated analog front-end (AFE) tailored for photoplethysmography (PPG) sensing. The AFE module introduces a novel transimpedance amplifier (TIA) that incorporates capacitive feedback techniques alongside common drain feedback (CDF) TIA. The unique TIA topology achieves both high gain and high sensitivity while maintaining low power consumption. The resultant PPG sensor module demonstrates impressive specifications, including an input noise current of 4.81 pA/sqrt Hz, a transimpedance gain of 18.43 MΩ, and a power consumption of 68 µW. Furthermore, the sensory system integrates an LED driver featuring automatic light control (ALC), which dynamically adjusts the LED power based on the strength of the received signal. Employing 0.35 µm CMOS technology, the AFE implementation occupies a compact footprint of 1.98 mm × 2.475 mm.
Alternative Cover Crops and Soil Management Practices Modified the Macronutrients, Enzymes Activities, and Soil Microbial Diversity of Rainfed Olive Orchards (cv. Chetoui) under Mediterranean Conditions in Tunisia
Authors: Elhaddad Fadoua , González Julio Antonio Calero , Abdelhamid Sofiane , Garcia-Ruiz Roberto , Chehab Hechmi
Published: June 2024

In Tunisia, the olive is the most cultivated fruit crop in the northern region, where annual rainfall exceeds 400 mm. This olive-growing area is characterized by a wide coverage of marginal soil with a high slope gradient. Therefore, the inclusion of cover crops in olive orchards is a sustainable solution to enhance ecosystem productivity, improve soil fertility, and increase oil yields. This study aimed to investigate the short-term (two cropping seasons in 2021 and 2022) effects of different seeded cover crops and soil management practices on soil characteristics, as well as soil health by measuring soil enzyme activities and microbial diversity. Six cover crop types consisting of wheat, vetch, oat, fenugreek, a vetch–oat mixture, and spontaneous vegetation were tested in association with rainfed olive trees (cv. Chetoui) in the north of Tunisia and compared to a control (which was tilled periodically three times per year without intercropping). During the first cropping season, cover crops were cut as animal feed, and only residues were incorporated into the soil. However, during the second year, all cover crop biomass was incorporated into the soil. The results indicated that the dry biomass production and carbon uptake were significantly higher in grass species (wheat and oat). All of the cover crops, including the spontaneous vegetation, significantly increased soil organic matter (SOM) and macronutrient levels, mainly, available phosphorus. On the other hand, the highest level of soil nitrogen was found in the fenugreek cover crop. The soil enzyme activities in the cover crops of wheat, oat, and the vetch–oat mix were higher than those in the control. Together with the increase in soil organic matter (SOM), this demonstrates a significant improvement in soil health with cover crops. Furthermore, this study proves that the utilization of carbon sources was dominated by amides, amines, and amino acids in the fenugreek plot, while it was dominated by polymers and carboxylic acids in the case of the wheat and oat. Overall, this study demonstrates that seeding cover crops is a sustainable management practice not only to integrate livestock but also to improve soil health in semiarid olive orchards.
Research on Optimization Strategies of Regional Cross-Border Transportation Networks—Implications for the Construction of Cross-Border Transport Corridors in Xinjiang
Authors: Dai Xiaomin , Liu Menghan , Lin Qiang
Published: June 2024

Facility connectivity plays a pioneering role in the Belt and Road Initiative proposed by General Secretary Xi Jinping in 2013. Xinjiang, as the core area of the Silk Road Economic Belt bordering eight Eurasian countries, plays a crucial role in cross-border transportation and humanistic exchanges and is the focus of the national connectivity initiative. While the current analysis on regional accessibility has become more diversified, analyses on long-distance cross-border corridors are still relatively rare. Therefore, this paper takes the Xinjiang Uygur Autonomous Region (XUAR) of China as the main study area extending westward to the five Central Asian countries. Modified accessibility accounting methods and gravity models are used to analyze the current status of accessibility and the strength of economic ties between Xinjiang and the five Central Asian countries. The results showed that the distance decay effect of transportation accessibility between Xinjiang and the five Central Asian countries is obvious; the constraints of “natural geography + transportation economy” affect the accessibility level from each state in Xinjiang to the five Central Asian countries and shows a trend of strength in the north and weakness in the south. From the optimization of the regional planning road network in a reverse projection, G3033 and other highways and the construction of the Yi-A railroad will improve the status quo of “east-west access but not north-south access” in Xinjiang. The “corridor effect” and spatial polarization characteristics of economic connection intensity from Xinjiang to the five Central Asian countries are significant. This study has important theoretical and practical significance for the construction of cross-border corridors.
A Sustainable mHealth Intervention to Promote Physical Activity for Healthy Aging: A Pilot Study of the “Every Walk You Take” Citizen Science Initiative
Authors: Naik Preet , Álamo-Junquera Dolores , Igual Laura , Serrajordi Marc , Pérez Albert , Pericas Carles , Pagès-Fernández Constança , Katapally Tarun Reddy , Grau María
Published: June 2024

The objective of the Every Walk You Take initiative was to co-design and test, in a pilot study, a sustainable mHealth intervention prototype (mobile app) to promote physical activity. This prototype would help to identify the barriers to and facilitators of active living in individuals older than 55 years. A prototype of the intervention was co-designed by a community of stakeholders in Barcelona who were familiar with the social and economic burden of aging in high-income societies. The app’s functionalities included recommendations for healthy routes in the city (parks, pedestrian lanes, and streets) according to environmental variables (air quality and climate) and personal preferences (route difficulty, distance, and geolocation), and ecological momentary assessments (pictures and voice notes) were collected to identify the barriers to and facilitators of performing these routes. To test the app, a pilot study was conducted over two 7-day cycles with citizen scientists recruited at the life-long learning centers of two deprived neighborhoods in Barcelona. A total of 21 citizen scientists (mean age = 67 (standard deviation = 7)), 86% of them female, collected 112 comments and 48 pictures describing their perceived barriers to and facilitators of active living. Every Walk You Take is a new, validated, and sustainable mHealth intervention that is directly involved in health promotion, as it empowers the citizens of Barcelona to play an active role in their own healthcare. This intervention has the potential to be implemented in different cities around the world to collect information on the community determinants of health and health assets.
Drivers of Value Creation and the Effect of ESG Risk Rating on Investor Perceptions through Financial Metrics
Authors: Puente De La Vega Caceres Abraham
Published: June 2024

This study delves deeply into the multifaceted nature of investor value creation, focusing on financial health, growth, profitability, cash flow, and ESG (environmental, social, and governance) risk ratings. The research employs partial least squares structural equation modeling (PLS-SEM) to dissect the interactions among these variables in a sample of 482 S&P 500 firms. Data were obtained from the FINRA database (2023) and Sustainalytics ESG risk ratings (2023). The results indicate that solid financial health enhances investor value creation. While growth fosters profitability, its direct impact on value creation and cash flow appears limited. The study also uncovers that ESG risk ratings negatively moderate the relationship between cash flow and value creation. This finding suggests that higher ESG risks lead to increased operational and compliance costs, which can reduce working capital and operating cash flow. Additionally, although sustainability investments may initially incur higher costs, they generate long-term value in terms of investment cash flow. A high perception of ESG risk can also raise financing costs, negatively impacting financial cash flow. These findings offer significant contributions to both academic theory and practical applications, shedding light on the complex interplay between financial and sustainability indicators in driving value creation for investors.
Bamboo Scrimber as a Sustainable Material for Chairs: A Property Study Based on the Finite Element Method
Authors: Duan Zhengjie , Zu Qing , Rao Fei
Published: June 2024

Bamboo scrimber is a sustainable biomass composite with physical and mechanical properties that has potential applications in furniture. However, its performance across different furniture needs evaluation considering the specific requirements for furniture with different functions. In this study, we simulated a traditional armchair model with bamboo scrimber, ash, or beech as the substrate. Using the finite element method, we analyzed stresses and deformations under six working conditions. The results show that bamboo scrimber had a lower maximum deformation and higher maximum stress under the vertical loading of the seat, backrest, legs, and armrests. Under armrest lateral loading, ash exhibited a higher maximum stress and lower maximum deformation. For selecting furniture material, we propose a strategy that optimizes furniture design by combining the advantages of traditional and new materials according to the structural characteristics and stresses of different parts of the furniture. The results confirm that bamboo scrimber has a good deformation resistance and structural stability and can be used as a substitute for traditional wood in furniture manufacturing, especially for chairs subjected to complex loads. Our findings will help to improve sustainable development by promoting the application of bamboo scrimber in the furniture manufacturing industry.
Collaborative Working and Critical Thinking: Adoption of Generative Artificial Intelligence Tools in Higher Education
Authors: Ruiz-Rojas Lena Ivannova , Salvador-Ullauri Luis , Acosta-Vargas Patricia
Published: June 2024

This study explores the impact of generative artificial intelligence tools on critical thinking and collaboration among university students, highlighting the importance of investigating these technologies due to their increasing integration into higher education and their potential to transform traditional pedagogical practices. A predominantly female sample was surveyed to assess their familiarity with and experience and perceptions of these tools. A total of 87% of the respondents had prior knowledge of generative AI tools, with 38% using them occasionally. Among the most popular tools are Canva 2024 (33%), Chat PDF (26%), and YOU.COM (24%). Additionally, 64% of the respondents believe that these tools significantly improve their critical thinking ability. Despite their high familiarity with and occasional use of these tools, the need for continuous training and technical support was identified. While generative AI tools show promising potential for enhancing collaboration and critical thinking in higher education, previous research has limitations, such as the lack of longitudinal data and the inadequacy in addressing ethical considerations and potential biases. More comprehensive research is needed to understand their long-term impact better and maximize their potential benefits.
Dried Rice for Alternative Feed as a Waste Management Product for Sustainable Bioeconomy in Rice-Producing Countries
Authors: Tonda Rusli , Hendroko Setyobudi Roy , Vincevica-Gaile Zane , Zalizar Lili , Roeswitawati Dyah , Ekawati Ida , Zekker Ivar , Burlakovs Juris , Iswahyudi Iswahyudi , Rudovica Vita
Published: June 2024

Dried rice, an organic waste recycling product, is made from dried rice leftovers. With a carbohydrate content nearly equivalent to corn but at a lower price, it has potential as an energy-generating feed, especially in poultry farming. The nutrient content and price of dried rice were evaluated to assess its efficiency for animal feed use. Dried rice samples from three areas in East Java, Indonesia, were analyzed for moisture, ash, crude protein, crude fat, and crude fiber content. Additionally, this research assesses the effectiveness of dried rice as a corn substitute in broiler feed by observing its impact on feed intake, average daily gain, feed conversion ratio, and broiler performance index. Proximate analysis showed insignificant differences among treatments, with moisture content ranging 12.45–12.71%, ash content 0.55–1.31%, crude protein 10.34–10.64%, crude fat 0.12–2.48%, and crude fiber from 0.81 to 1.55%. Although all samples were assessed as efficient, products from Lumajang and Pasuruan were preferred for feed production due to their similarity to corn nutrient content. Dried rice costs approximately USD 213–228 per ton, significantly lower than corn. Dried rice production reduces both organic waste and poultry production costs concurrently, serving as a sustainable waste management model in Indonesia and other rice-producing countries, shifting towards a bioeconomy from a linear economy.
Prescribed Performance Spacecraft Attitude Control with Multiple Convergence Rates
Authors: Gui Mingzhen , Dai Ming-Zhe , Zhang Chengxi , Zhang Xing , Wu Jin
Published: June 2024

This paper proposes a prescribed performance control policy for spacecraft attitude tracking control. The designed controller employs a kind of performance envelope function that can configure the convergence rate arbitrarily. Moreover, the controller design is based on the barrier Lyapunov function and indirect adaptive methods, which can suppress the fluctuation of external disturbances to obtain lower steady-state errors. Compared to prior studies on spacecraft attitude tracking, the proposed policy can arbitrarily configure the system convergence performance to achieve performance trade-offs in different scenarios. Moreover, the barrier Lyapunov function design and adaptive methods can achieve low steady-state errors with low computational resource consumption. Simulations that verify its effectiveness and superiority are provided herein.
Development of Biodegradable Bioplastics with Sericin and Gelatin from Silk Cocoons and Fish Waste
Authors: Vijayakumar Natesan , Sanjay Aathiyur Velumani , Al-Ghanim Khalid A. , Nicoletti Marcello , Baskar Gurunathan , Kumar Ranvijay , Govindarajan Marimuthu
Published: June 2024

The bioplastics sector promotes environmentally friendly means of cutting down on the usage of fossil fuels, plastic waste, and environmental pollution. Plastic contamination has detrimental effects on both ecological systems and the global food supply. The approach we present here to resolve this issue involves the integration of sericin and gelatin, obtained from cocoon and fish waste, respectively, with nano-reinforced cellulose crystals, to develop a biodegradable and compostable plastic material. The use of cocoon and fish wastes for the extraction of sericin and gelatin presents an environmentally beneficial approach since it contributes to waste reduction. The sericin level found in silk cocoon waste was determined to be 28.08%, and the gelatin amount in fish waste was measured to be 58.25%. The inclusion of sericin and gelatin in bioplastics was accompanied by the incorporation of glycerol, vinegar, starch, sodium hydroxide, and other coloring agents. Fourier transform infrared (FTIR) examination of bioplastics revealed the presence of functional groups that corresponded to the sericin and gelatin components. The tensile strength of the bioplastic material was measured to be 27.64 MPa/psi, while its thickness varied between 0.072 and 0.316 mm. The results of burial experiments indicated that the bioplastic material had a degradation rate of 85% after 14 days. The invention exhibits potential as a viable alternative for packaging, containment, and disposable plastic materials. The use of this sustainable approach is recommended for the extraction of sericin and gelatin from silk cocoons and fish waste, with the intention of using them as raw materials for bioplastic production.
Mismanagement of SARS-CoV-2 Infection Pre Hospitalisation during the Omicron Era: Antibiotics and Steroids Instead of Early Antivirals
Authors: Giacomelli Andrea , Ciubotariu Cosmin Lucian , Zacheo Martina , Rabbione Andrea , Pieruzzi Margherita , Barone Federico , Poloni Andrea , Casalini Giacomo , Pozza Giacomo , Colaneri Marta , Passerini Matteo , Ridolfo Anna Lisa , Gervasoni Cristina , Cattaneo Dario , Gori Andrea , Antinori Spinello
Published: June 2024

The aim of this study was to assess the prevalence of inappropriate treatment among hospitalised patients affected by SARS-CoV-2 infection before hospital admission during the Omicron era. This single-centre, retrospective observational study included all the patients hospitalised because of SARS-CoV-2 infection during three periods characterised by the Italian prevalence of an Omicron variant of concern: (1) January–May 2022 (BA.1–BA.2), (2) June–October 2022 (BA.5), and (3) November 2022–March 2023 (BQ.1-XBB). Inappropriate treatment was defined as pre-hospitalisation exposure to antibiotics and/or steroids in the absence of a documented bacterial infection or the need for steroid treatment of an underlying medical condition. A total of 931 subjects were hospitalised: 394 in period 1, 334 in period 2, and 203 in period 3. Of the 157 patients undergoing inappropriate treatment (16.9%), 142 (15.3%) received antibiotics and 52 (5.6%) steroids. The proportion of inappropriately treated patients significantly decreased over time, from 23.1% in period 1 to 11.7% in period 2 and 13.3% in period 3 (p < 0.001), and there was a parallel decrease in antibiotic (p < 0.001) and steroid treatment (p < 0.013). Only 13 subjects (1.4%) received early pre-hospitalisation treatment for SARS-CoV-2. A significant proportion of hospitalised COVID-19 patients were exposed to inappropriate treatment before hospital admission.
Engineered Resistance to Tobamoviruses
Authors: Carr John Peter
Published: June 2024

Tobacco mosaic virus (TMV) was the first virus to be studied in detail and, for many years, TMV and other tobamoviruses, particularly tomato mosaic virus (ToMV) and tobamoviruses infecting pepper (Capsicum spp.), were serious crop pathogens. By the end of the twentieth and for the first decade of the twenty-first century, tobamoviruses were under some degree of control due to introgression of resistance genes into commercial tomato and pepper lines. However, tobamoviruses remained important models for molecular biology, biotechnology and bio-nanotechnology. Recently, tobamoviruses have again become serious crop pathogens due to the advent of tomato brown rugose fruit virus, which overcomes tomato resistance against TMV and ToMV, and the slow but apparently inexorable worldwide spread of cucumber green mottle mosaic virus, which threatens all cucurbit crops. This review discusses a range of mainly molecular biology-based approaches for protecting crops against tobamoviruses. These include cross-protection (using mild tobamovirus strains to ‘immunize’ plants against severe strains), expressing viral gene products in transgenic plants to inhibit the viral infection cycle, inducing RNA silencing against tobamoviruses by expressing virus-derived RNA sequences in planta or by direct application of double-stranded RNA molecules to non-engineered plants, gene editing of host susceptibility factors, and the transfer and optimization of natural resistance genes.
Exploration of Potential Broad-Spectrum Antiviral Targets in the Enterovirus Replication Element: Identification of Six Distinct 5′ Cloverleaves
Authors: Daniels Morgan G. , Werner Meagan E. , Li Rockwell T. , Pascal Steven M.
Published: June 2024

Enterovirus genomic replication initiates at a predicted RNA cloverleaf (5′CL) at the 5′ end of the RNA genome. The 5′CL contains one stem (SA) and three stem-loops (SLB, SLC, SLD). Here, we present an analysis of 5′CL conservation and divergence for 209 human health-related serotypes from the enterovirus genus, including enterovirus and rhinovirus species. Phylogenetic analysis indicates six distinct 5′CL serotypes that only partially correlate with the species definition. Additional findings include that 5′CL sequence conservation is higher between the EV species than between the RV species, the 5′CL of EVA and EVB are nearly identical, and RVC has the lowest 5′CL conservation. Regions of high conservation throughout all species include SA and the loop and nearby bases of SLB, which is consistent with known protein interactions at these sites. In addition to the known protein binding site for the Poly-C binding protein in the loop of SLB, other conserved consecutive cytosines in the stems of SLB and SLC provide additional potential interaction sites that have not yet been explored. Other sites of conservation, including the predicted bulge of SLD and other conserved stem, loop, and junction regions, are more difficult to explain and suggest additional interactions or structural requirements that are not yet fully understood. This more intricate understanding of sequence and structure conservation and variability in the 5′CL may assist in the development of broad-spectrum antivirals against a wide range of enteroviruses, while better defining the range of virus isotypes expected to be affected by a particular antiviral.
Molecular Survey on the Occurrence of Tick-Borne Bacteria in Wild Birds from Central Italy
Authors: Bertelloni Fabrizio , Cagnoli Giulia , Interrante Paolo , Ceccherelli Renato , Ebani Valentina Virginia
Published: June 2024

Birds are known to be carriers of ticks infected by tick-borne pathogens, including bacteria. However, not many studies have been carried out on avian tissues to detect these agents. The aim of the present survey was to investigate, using PCR, the presence of Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Chlamydia psittaci, Coxiella burnetii, Ehrlichia canis, Francisella tularensis, and Rickettsia spp. in the spleens collected from 300 wild birds of different orders and species from Central Italy. A total of 53 (17.67%) samples were PCR positive for at least one investigated pathogen. One (0.33%) bird was positive for Bartonella spp., five (1.67%) birds were positive for C. burnetii, eleven (3.67%) for B. burgdorferi s.l., and thirty-six (12%) for C. psittaci. No coinfection was detected. All samples were negative for A. phagocytophilum, E. canis, F. tularensis, and Rickettsia spp. The findings showed that wild birds may harbor different zoonotic tick-borne bacteria; therefore, they can contribute to the diffusion of these agents.
Molecular Fingerprinting of the Biodegradation of Petroleum Organic Pollutants in Groundwater and under Site-Specific Environmental Impacts
Authors: Yang Mingxing , Yang Yuesuo , Yang Xinyao , Song Xiaoming , Du Xinqiang , Lu Ying
Published: June 2024

A quantitative and qualitative assessment using molecular markers derived from compound-specific indices for indicating groundwater impacted by petroleum spills in an oil field was recently undertaken and demonstrated serious contamination, with both high total petroleum hydrocarbons (TPH) (3.68–7.32 mg/L) and hazardous compounds in the groundwater. A petroleum source was identified, and the analysis revealed a decreasing trend of fresh petroleum input, along with groundwater advection and an increasing trend of biodegradation potential at locations farther from the source. This was confirmed via microbial analysis with both biodegrading microorganisms and diversity indices (Shannon, Simpson, and Pielou) and the principal component analysis (PCA) modeling approach, which classified the field samples into three types according to the distribution correlations between different organic compounds. Biodegradation was believed to be the dominant sink of hydrocarbons due to the increasing Pr/C17 and Ph/C18 values with seasonal changes. Raised temperatures activated the microbial degradation process; specifically, low-weight hydrocarbons degraded more rapidly than high-weight hydrocarbons, resulting in the accumulation of an unresolved complex mixture of bioproducts at locations that were farther away. Spatially, the Pr/C17 and Ph/C18 values increased from the upstream to the downstream areas, showing substantial biodegradation. The relationships between the molecular markers and chemical indices were quantified via canonical correlation analysis (CCA) to visually explain the interactive reaction processes. It was also demonstrated that the biodegradation of petroleum organics can be characterized by the consumption of dissolved oxygen and a decreasing Pr/Ph ratio, due to system reduction. These results demonstrate that compound-specific molecular markers, coupled with biochemical parameters, can effectively support a better understanding and effective fingerprinting of the fate and transport of petroleum organic contaminants, thus offering valuable technical support for a cost-effective remediation strategy.
Effect of Median Soil–Particle Size Ratio on Water Storage Capacity of Capillary Barrier
Authors: Liu Honghua , Dong Jie , Liu Qiang , Geng Lin , Wang Zhongsheng , Sun Chong
Published: June 2024

Capillary barriers are widely used as a cover system to enhance the upper-soil-layer water storage capacity and reduce water infiltrate into the lower soil layer. In this paper, the effects of the median soil–particle size ratio on the water storage capacity of capillary barriers were studied using a series of indoor one-dimensional soil column infiltration tests. The results show that the water storage capacity rises with an increase in the median soil–particle size ratio until it exceeds 10. The variation in the total water storage capacity is related to not only the median soil–particle size ratio but also the particle size of coarse-grained soil or fine-grained soil. When the fine-grained soil-layer particle size is constant, the total water storage first increases, then decreases, and finally remains constant after increasing the median soil–particle size ratio. In contrast, when the coarse-grained soil layer particle size is constant, the relationship between the capillary barrier’s total water storage and median soil–particle size ratio can be defined as a power function. Using the capillary barrier can increase coarse-grained sand by 90% in water storage capacity and can only increase fine-grained sand by 7% in water storage capacity. The breakthrough time increases with the increase in the median soil–particle size ratio. The presence of the coarse and fine-grained soil layer interface in the capillary barrier can affect the fine-grained soil layer infiltration rate.
Changing Soil Water Content: Main Trigger of the Multi-Phase Mobilization and Transformation of Petroleum Pollution Components—Insights from the Batch Experiments
Authors: Yang Mingxing , Wang Bing , Xia Yubo , Qiu Yan , Li Chunling , Cao Zhendong
Published: June 2024

Soil moisture content continuously alters the types and forms of petroleum organic pollutants in the soil through processes such as dissolution, convection, and dilution, forming complex migration and transformation in a water–air–soil–NAPL system. Field investigations and traditional indoor simulation experiments have difficulty in terms of accurately diagnosing the state of different petroleum pollutants due to the influence of environmental factors and the difficulty of controlling single factors. Batch experiments were conducted to simulate the mobilization and differentiation processes of petroleum pollutants under the influence of soil water content. The results show that (1) the residual content of components is the lowest in coarse sand and the highest in clay, which is mainly affected by soil particles; meanwhile, the residual saturation value of octanoic acid is the largest, and that of toluene is the smallest, as determined in terms of their viscosity and volatility. (2) The infiltration processes of the components are affected by their properties and medium characteristics. Due to its small particle size and strong adsorption, clay has the highest residual saturation of petroleum pollutants (28.8%). This can even be more than twice that of coarse sand (13.3%). For different components, the residual saturations of octanoic acid and toluene are the highest and lowest, respectively (taking fine sand as an example: 25.3% and 13.2%), with a relatively large difference, as determined in terms of viscosity and solubility. (3) As the free phase can migrate freely, it is transformed most rapidly in the pores. The changes in the dissolved phase of each component are relatively small and tend to be gentle. The changes in the residual phase are mainly affected by volatility, viscosity, soil particles, and pore and cosmid content; the degree of change is ordered as follows: toluene > cyclohexane > hexadecane > octanoic acid.
Numerical Study of Low-Specific-Speed Centrifugal Pump Based on Principal Component Analysis
Authors: Wei Yangyang , Zhu Han , Fan Quanwang , Qiu Ning , Wu Jie , Zhang Weibin
Published: June 2024

The characteristics of pressure pulsations in centrifugal pumps have attracted considerable attention. In this study, principal component analysis is used to discuss the pressure pulsations in a centrifugal pump with a low specific speed, and the primary causes for these pressure pulsations are analyzed in conjunction with experimental results. The results indicate that principal component analysis effectively separates the primary modes that influence the flow field characteristics. An excessive wrap angle results in the formation of a backflow vortex on the working face of the blade. Obvious stratification of the zero-order modal pressure indicates that the geometric structure of the impeller is rational and that the transient flow field is stable. The second- and third-order modes are conjugates, and their dominant frequency coincides with the dominant rotating frequency of the impeller, indicating that the pulsations of a single channel are the primary component of the pressure pulsations. The primary frequency (148.54 Hz) of the pressure pulsations at monitoring points distributed across the volute is three times the rotational frequency (49.51 Hz) of the impeller. The different positions and sub-frequencies of the monitoring points mean that the principal component analysis can effectively identify the impeller-induced sub-frequency difference.
Eleştirel Düşünme Perspektifi Kazandırmada Bazı Kur’an Kıssaları ve Temel Parametreleri
Authors: Tekingür Sadık
Published: June 2024

Öz
Kur’an, tarihsel süreç içerisinde yaşamış insan topluluklarının geçirdiği hayat deneyimlerini sonraki kuşaklara aktarmak amacıyla ontik/varoluşsal, epistemik/bilişsel ve ahlâki/etik değerlere olumlu-olumsuz bir tarzda yaklaşmaktadır. Kur’an’ın varlığa dair sunduğu ontik/ontolojik ve inanç temelli eleştirel yaklaşımlar, varlığın ontik gerçekliği dışında algılayan ve algılanan bireyin bilişsel/epistemik yönüyle özne ve nesnesine yapılan eleştirel-metodik sorgulamalar ve toplumların içinde bulunduğu ahlâki tutumlarına yönelik sistematik ve yön gösterici eleştirel perspektifler, Kur’an kıssalarında sonraki kuşakların eğitilmesinde önemli bir işlev görmektedir. Kur’an, inanç ile ilgili hususları kıssalarda bazen somutlaştırma, bazen gözlem ve deneye başvurma bazen Sokratik yöntem ve bazen de örnek olay gösterme metoduyla inananların idrakine sunmakta; bilişsel yönü ağır basan muhtevaların hem özne hem de nesnesine soru-cevap şeklinde, yeri geldiğinde düşünme ve türevlerine vurgu yapmak suretiyle özneye bir bakış açısı kazandırmakta; ahlâki tutum ve değerlere yönelik ise bazen karşıtını göstermek suretiyle bazen de olanı olduğu gibi resmetmekle, bazen en ideali göstermekle olumsuz tavır içinde olanların kötü ahlâki tutumlarını yermektedir. Yer yer ise özelden genele akıl yürütme, farklı tutumlar arası karşılaştırmalar yapma, benzerlik ve farklılıkları saptayabilme, tutarsızlıkları ortaya çıkarma, ben merkezli düşünmeden ortak aklı çalıştıracak bir konsensüse varma, olaylar arası analitik ve sentetik düşünme becerilerini geliştirme eleştirel bir bakışın kazanılmasında önem arz etmektedir. Kur’an kıssalarında önceden meydana gelen hata ve yanlışlara düşülmesini engellemek, sorgulayan bir zihinle olay ve hadiseleri ele alıp irdelemek, olanı olduğu şekliyle ele alıp nesnel bir biçimde ve aklın düşünme süreçlerini aktive ederek yaklaşılmasını temin etmek eleştirel bakışın sağladığı işlevlerdir. Bu bağlamda Kur’an kıssalarının kazandırdıkları eğitsel hedefler; inananlara teselli verme, inancı sağlamlaştırma, mevcut yaklaşıma eleştirel bakabilme, peygamberlerin iddiasını ispat etme, Tevhid anlayışını hâkim kılma, imana çağrılan insanları tarihsel gerçeklerle yüzleştirme, muhatapları düşünmeye sevk ederek ders ve ibret almalarını sağlamaktır.
Anahtar Kelimeler; Din eğitimi, Kur’an, Kur’an kıssaları, eleştirel düşünme
Combination of mesenchymal stem cell sheet with poly-caprolactone nanofibrous mat and Gelfoam increased osteogenesis capacity in rat calvarial defect
Authors: Banimohamad-Shotorbani Behnaz , Rahbarghazi Reza , Jarolmasjed Seyedhosein , Mehdipour Ahmad , Shafaei Hajar
Published: June 2024

Introduction: To date, different strategies have been used for co-transplantation of cell-loaded biomaterials for bone tissue regeneration. This study aimed to investigate the osteogenic properties of adipose-derived-mesenchymal stem cell (AD-MSC) sheets combined with nanofibrous poly-caprolactone (PCL) mat and Gelfoam in rats with calvarial bone defect. Methods: Calvarial critical-size defects were induced in male rats. Animals were classified into Control, Gelfoam, Gelfoam/PCL nanofiber, Gelfoam/AD-MSC sheet, and Gelfoam/PCL nanofiber/AD-MSC sheet groups. After 3 months, rats were sacrificed and the regeneration rate was evaluated. Results: Almost all groups showed bone regeneration properties, but the volume of newly formed bone was higher in groups that received Gelfoam/AD-MSC and Gelfoam/PCL nanofiber/AD-MSC sheets (P < 0.05). The application of Gelfoam/PCL nanofiber/AD-MSC sheets not only increased bone thickness, bone volume/total bone volume (BV/TV) ratio, strong Hounsfield Unit (HU), but also led to the formation of ossified connective tissue with wrinkled patterns. Conclusion: The current study indicated that the Gelfoam/PCL nanofiber/AD-MSC sheet provides a suitable platform for effective osteogenesis in calvarial bone defects.
Retinoic acid-releasing scaffold based on chitosan hydrogel and testis decellular plates
Authors: Zarei Hooman , Movahedin Mansoureh , Ganji Fariba , Ghiaseddin Ali
Published: June 2024

Introduction: The use of releasing scaffolds is promising for testes tissue engineering. Chitosan (CS) is a natural biopolymer extensively used as a delivery system. The decellularized testis provides a structure resembling natural extracellular matrix (ECM). All-trans retinoic acid (atRA) is an important factor for spermatogonia differentiation, meiosis completion, and mature sperm release. In this study, thermosensitive CS/βGP hydrogel was served as a novel atRA-releasing support for testis decellular plates (TDPs). Methods: The CS/βGP hydrogel was evaluated for gelation time, morphology, wettability, cytocompatibility, and atRA-releasing behavior. Mouse testes were treated with 1% SDS and evaluated for decellularization efficacy through morphological assessments, DNA content assays, and DAPI staining. TDPs were obtained from the decellularized testes and placed on an atRA-releasing CS/βGP hydrogel support. Results: The CS/βGP hydrogels were prepared with different formulations. It was found that increasing the βGP concentration significantly decreased the gelation time. The addition of atRA did not considerably affect the hydrophilicity of hydrogel. The in vitro release studies showed a sustained atRA release behavior, although an initial low burst release was recorded. Also, increasing the amount of atRA led to a decrease in the rate of drug release. The decellularization procedure successfully removed cells while preserving the ECM. The atRA-releasing CS-TDP scaffold was found to be non-toxic with good biocompatibility. Conclusion: Results showed that the novel atRA-releasing CS-TDP scaffold can sustainably deliver atRA to the culture system and create a cytocompatible environment for testicular cells. Therefore, this scaffold may be useful in developing new tissue engineering approaches for various types of male infertility diseases.
Topically applied GHK as an anti-wrinkle peptide: Advantages, problems and prospective
Authors: Mortazavi Seyedeh Maryam , Mohammadi Vadoud Seyyed Ali , Moghimi Hamid Reza
Published: June 2024

Introduction: Peptides are promising and attractive anti-wrinkle active ingredients, amongst which glycyl-histidyl-lysine peptide (GHK) is one of the most broadly promoted peptide for topical application. This simple sequence of amino acid residues not only has the capability of tissue regeneration and the enhancement of collagen and glycosaminoglycans synthesis but also is able to increase nerve outgrowth and angiogenesis. Consequently, GHK has several properties, from wound healing to prevention/reduction wrinkles. GHK-Cu and Pal-GHK are metal complex and palmitoylated derivatives of GHK, respectively. Although GHK-Cu and Pal-GHK are widely used in anti-wrinkle products available on the cosmetic market, the published information on their skin permeability, effectiveness, physicochemical properties and so on is insufficient. Methods: This review aims to highlight whether GHK is sufficiently effective on wrinkle prevention/reduction. Apart from the effectiveness, another question that is tried to be answered is whether skin permeability of GHK allows it to act as an anti-wrinkle peptide at its site of action? Skin permeation enhancement methods employed so far are also reviewed. Results: Based on cellular studies, undoubtedly, GHK can be considered as an anti-wrinkle ingredient. Although GHK-Cu and Pal-GHK have been of interest as effective peptides to be incorporated in the anti-wrinkle products, there is a surprising absence of clinical studies using them. Metal complexation and chemical modification with a hydrophobic moiety increase permeability of this peptide. Besides, cell penetrating peptides seem promising to increase skin permeation of GHK and its derivatives. Skin pretreatment with microneedles also has the potential to be further studied for permeation enhancement of such peptides. As peptide ingredients, their formulation may encounter some challenges, mainly due to their hydrophilic (high aqueous solubility and low partition coefficient) and unstable nature. Conclusion: Although GHK-Cu and Pal-GHK are effective and relatively skin permeable, their permeability could be successfully increased using permeation enhancement methodologies.
A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models
Authors: Jamshidi Nazanin , Jamshidi Negar , Modarresi Chahardehi Amir , Shams Elahe , Chaleshi Vahid
Published: June 2024

Pancreatic ductal adenocarcinoma (PDAC) stands as the fourth leading cause of cancer-related deaths, primarily attributable to its resistance to chemotherapy, resulting in a nearly universal fatality rate. Despite the promise exhibited by numerous drugs in preclinical studies, their subsequent failure in clinical trials underscores the inherent limitations of conventional two-dimensional cell culture models commonly employed in early drug screening endeavors. The inadequacies of two-dimensional (2D) models prompted the exploration of three-dimensional (3D) culture systems, which more faithfully recapitulate the native tumor microenvironment. These 3D systems have distinct advantages over 2D models in morphology, proliferation, drug response, and protein expression. Among these 3D platforms, tumor organoids and spheroids, generated through different methodologies, have emerged as next-generation models that closely mirror aspects of pancreatic tumor biology. This comprehensive review scrutinizes pancreatic cancer spheroids' techniques, tissue sources, and applications, offering a nuanced analysis of their advantages and limitations. By comparing these distinct 3D culture systems, researchers gain valuable insights to inform the selection of optimal model designs aligned with their specific experimental objectives. The utilization of these advanced models holds significant promise for enhancing the clinical relevance of both in vitro and in vivo cancer research, thereby contributing to the development of improved therapeutics against pancreatic cancer.