bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation of Ventilative Cooling Potentials and Building Energy Savings in Various Climatic Zones
Authors: Rahman Haolia , Han Hwataik
Published: March 2019

The introduction of cool outdoor air can help in reducing the energy consumption for cooling during summer. Ventilative cooling potentials (VCPs) have been defined in various ways in the literature to represent potential cooling hours in specified outdoor temperature ranges. However, the energy-saving potential of ventilative cooling can differ between buildings in the same climatic zone depending on the buildings’ thermal characteristics and system operations. In this study, new VCPs are introduced with an index of temperature shift based on adaptive thermal comfort. This index can be determined based on the balance temperature difference of the buildings, which is defined as the heat gain in the building divided by the thermal transmission and air exchange characteristics of the building envelope under quasi-steady state conditions. The proposed method was also compared with those reported in the literature, including a computer-based VCP tool. It is the objective of the present study to investigate the correlation between VCPs and actual energy savings via ventilative cooling. Simulations were conducted in an office building for a four-month period during summer to calculate the energy saved via ventilative cooling in comparison with that achieved with a mechanical cooling system. Eight cities representing four different climatic conditions were considered: tropical, dry, temperate, and continental. Our results revealed a strong correlation between the energy savings and the proposed VCPs in the case of a proper temperature shift estimation in all climatic zones. The computerized VCP tool also exhibited good correlation with the calculated energy savings and with the VCPs proposed herein.
Modeling and Optimization of Dual Active Bridge DC-DC Converter with Dead-Time Effect under Triple-Phase-Shift Control
Authors: Song Chaochao , Chen Alian , Pan Yiwei , Du Chunshui , Zhang Chenghui
Published: March 2019

Dead-time effect has become an apparent issue in high-switching-frequency high-power dual active bridge (DAB) DC-DC converter. This paper gives a detailed analysis of phase-shift errors effect caused by dead time, including output voltage offset, soft-switching failure, optimal scheme failure, etc. Phase-shift errors effect will invalidate traditional analyses of optimal control and mislead the design of DAB converter. To overcome these drawbacks, various operating modes and an accurate transmission power model incorporating dead time under triple-phase-shift (TPS) control are developed. On this basis, an optimal TPS incorporating dead time (TPSiDT) scheme is further proposed to minimize the current stress, while guaranteeing soft-switching operation by using Lagrange multiplier method (LMM) and Genetic Algorithm (GA). The novel transmission power model can provide accurate power flow computation to avoid phase-shift errors. Therefore, in practical applications, the minimum current stress and soft-switching operation can be guaranteed, and the efficiency of DAB converter can be improved. Finally, the experimental results verify the feasibility of the proposed TPSiDT scheme.
High-Efficiency Design and Control of Zeta Inverter for Single-Phase Grid-Connected Applications
Authors: Choi Woo-Young , Yang Min-Kwon
Published: March 2019

The conventional zeta inverter has been used for single-phase grid-connected applications. However, it has high switching losses to operate at high switching frequency in the continuous conduction mode (CCM). To address this drawback, this paper suggests a high-efficiency zeta inverter using active clamp and synchronous rectification techniques. The proposed inverter utilizes the active clamp circuit for reducing switching losses. The non-complementary switching scheme is adopted for not only clamping the switch voltage stresses, but also alleviating the circulating energy. In addition, the synchronous rectification is implemented for reducing the body diode conduction of power switches. By using the silicon carbide (SiC) metal oxide semiconductor field effect transistors (MOSFETs), the switching performance of the proposed inverter is improved. Its operation principle and control strategy are presented. A 220-W prototype has been designed and tested to evaluate the performance of the proposed inverter.
A New Hybrid Multilevel Inverter Topology with Reduced Switch Count and dc Voltage Sources
Authors: Bassi Hussain Mohammad , Salam Zainal
Published: March 2019

In this paper, a new single-phase hybrid multilevel inverter (MLI) is proposed. Compared to other existing MLI topologies, the proposed circuit is capable of producing a higher number of output voltage levels using fewer power switches and dc sources. The levels are synthesized by switching the dc voltage sources in series/parallel combinations. An auxiliary circuit is introduced to double the number of levels by creating an intermediate step in between two levels. In addition, a zero level is introduced to overcome the inherent absence of this level in the original circuit. To improve the total harmonic distortion, a hybrid modulation technique is utilized. The operation and performance of the circuit are analyzed and confirmed using MATLAB/Simulink simulation. To validate the workability of the proposed idea, a 300 W, a thirteen level MLI (including the zero level) is designed and constructed. The circuit is tested with a no-load, resistive load and resistive-inductive load. The experimental results match very closely with the simulation and mathematical analysis.
Thermodynamics and Kinetics of CO2/CH4 Adsorption on Shale from China: Measurements and Modeling
Authors: Chi Yuan , Zhao Changzhong , Lv Junchen , Zhao Jiafei , Zhang Yi
Published: March 2019

CO2-enhanced shale gas recovery (CO2-ESGR) sequestrates anthropogenic CO2 and improves the profitability of shale gas exploitation. This work investigated the adsorption behaviors of CO2 and CH4 on shale from China at 20, 40, 60 and 80 °C. The pressure ranges for CO2 and CH4 were 1–5 and 1–15 MPa, respectively. The excess adsorbed amount of CH4 increased with increasing pressure from the beginning to the end, while the maximum excess CO2 adsorption was observed at approximately 4 MPa. The absolute average deviations (AADs) of CO2 and CH4, determined by the Langmuir + k model, were 2.12–3.10% and 0.88–1.11%, respectively. Relatively good adsorptivity for CO2 was exhibited when the pressure was less than 5 MPa, which was beneficial to the implementation of CO2-ESGR. With continuous increases in pressure, the adsorption capacity of CO2 was weaker than that of CH4, suggesting that the injected CO2 would reduce the partial pressure of CH4 for CO2-ESGR and the displacement effect would no longer be significant. In addition, the adsorption rate of CO2 was much faster than that of CH4. CO2 was more active in the competitive adsorption and it was advantageous to the efficiency of CO2-ESGR.
Italian Biogas Plants: Trend, Subsidies, Cost, Biogas Composition and Engine Emissions
Authors: Benato Alberto , Macor Alarico
Published: March 2019

Italy is one of the leading nations in the biogas sector. Agricultural, landfill, sewage and manure substrates are converted into biogas using anaerobic digestion and, then, into electricity and heat by means of properly arranged internal combustion engines. In this study, after an overview of the European context, the authors present the Italian biogas sector status in terms of development trends and factors that favour/block biogas spread. Despite the fact that biogas is a renewable fuel and a consolidate technology, it is mandatory to examine its real costs, biogas composition and engine combustion products. For this purpose, in the present work, the authors selected six in-operation biogas plants fed by different substrates, investigate plants construction and operation costs and measure both biogas and engine emissions compositions. Biogas status analysis shows a high growth rate until the end of 2012 due to generous Government subsidies while, after supports reduction, a continuous depletion of biogas installations is observed. Alongside the development, established supports overlook also the plant size as well as the cost. In fact, the most widespread plant nameplate electric power is 1 MW while its construction cost ranges between 4.2–4.8 millions of Euros. Real on-site measurements show variable biogas composition while engine emissions are comparable with the natural gas ones.
The Influence of Surface Radiation on the Passive Cooling of a Heat-Generating Element
Authors: Miroshnichenko Igor V. , Sheremet Mikhail A. , Mohamad Abdulmajeed A.
Published: March 2019

Low-power electronic devices are suitably cooled by thermogravitational convection and radiation. The use of modern methods of computational mechanics makes it possible to develop efficient passive cooling systems. The present work deals with the numerical study of radiative-convective heat transfer in enclosure with a heat-generating source such as an electronic chip. The governing unsteady Reynolds-averaged Navier–Stokes (URANS) equations were solved using the finite difference method. Numerical results for the stream function–vorticity formulation are shown in the form of isotherm and streamline plots and average Nusselt numbers. The influence of the relevant parameters such as the Ostrogradsky number, surface emissivity, and the Rayleigh number on fluid flow characteristics and thermal transmission are investigated in detail. The comparative assessment clearly emphasizes the effect of surface radiation on the overall energy balance and leads to change the mean temperature inside the heat generating element. The results of the present study can be applied to the design of passive cooling systems.
Simulation of Surfactant Oil Recovery Processes and the Role of Phase Behaviour Parameters
Authors: Druetta Pablo , Picchioni Francesco
Published: March 2019

Chemical Enhanced Oil Recovery (cEOR) processes comprise a number of techniques which modify the rock/fluid properties in order to mobilize the remaining oil. Among these, surfactant flooding is one of the most used and well-known processes; it is mainly used to decrease the interfacial energy between the phases and thus lowering the residual oil saturation. A novel two-dimensional flooding simulator is presented for a four-component (water, petroleum, surfactant, salt), two-phase (aqueous, oleous) model in porous media. The system is then solved using a second-order finite difference method with the IMPEC (IMplicit Pressure and Explicit Concentration) scheme. The oil recovery efficiency evidenced a strong dependency on the chemical component properties and its phase behaviour. In order to accurately model the latter, the simulator uses and improves a simplified ternary diagram, introducing the dependence of the partition coefficient on the salt concentration. Results showed that the surfactant partitioning between the phases is the most important parameter during the EOR process. Moreover, the presence of salt affects this partitioning coefficient, modifying considerably the sweeping efficiency. Therefore, the control of the salinity in the injection water is deemed fundamental for the success of EOR operations with surfactants.
Early Growth Response of Nine Timber Species to Release in a Tropical Mountain Forest of Southern Ecuador
Authors: Cabrera Omar , Fries Andreas , Hildebrandt Patrick , Günter Sven , Mosandl Reinhard
Published: March 2019

Research Highlights: This study determined that treatment “release from competitors” causes different reactions in selected timber species respective to diametrical growth, in which the initial size of the tree (diametric class) is important. Also, the growth habit and phenological traits (defoliation) of the species must be considered, which may have an influence on growth after release. Background and Objectives: The objective of the study was to analyze the diametric growth of nine timber species after their release to answer the following questions: (i) Can the diametric growth of the selected timber species be increased by release? (ii) Does the release cause different responses among the tree species? (iii) Are other factors important, such as the initial diameter at breast height (DBH) or the general climate conditions? Materials and Methods: Four-hundred and eighty-eight trees belonging to nine timber species were selected and monitored over a three-year period. Release was applied to 197 trees, whereas 251 trees served as control trees to evaluate the response of diametrical growth. To determine the response of the trees, a linear mixed model (GLMM, R package: LMER4) was used, which was adjusted by a one-way ANOVA test. Results: All species showed a similar annual cycle respective to diametric increases, which is due to the per-humid climate in the area. Precipitation is secondary for the diametric growth because sufficient rainfall occurs throughout year. What is more important, however, are variations in temperature. However, the species responded differently to release. This is because the initial DBH and growth habit are more important factors. Therefore, the species could be classified into three specific groups: Positive, negative and no response to release. Conclusions: Species which prefer open sites responded positively to release, while shade tolerant species and species with pronounced phenological traits responded negatively. The initial DBH was also an important factor for diametric increases. This is because trees of class I (20 cm to 30 cm DBH) responded positively to the treatment, whereas for bigger or older individuals, the differences decreased or became negative.
Bioactive Compounds, Nutritional Value, and Potential Health Benefits of Indigenous Durian (Durio Zibethinus Murr.): A Review
Authors: A Aziz Nur Atirah , Mhd Jalil Abbe Maleyki
Published: March 2019

Durian (Durio zibethinus Murr.) is an energy-dense seasonal tropical fruit grown in Southeast Asia. It is one of the most expensive fruits in the region. It has a creamy texture and a sweet-bitter taste. The unique durian flavour is attributable to the presence of fat, sugar, and volatile compounds such as esters and sulphur-containing compounds such as thioacetals, thioesters, and thiolanes, as well as alcohols. This review shows that durian is also rich in flavonoids (i.e., flavanols, anthocyanins), ascorbic acid, and carotenoids. However, limited studies exist regarding the variation in bioactive and volatile components of different durian varieties from Malaysia, Thailand, and Indonesia. Experimental animal models have shown that durian beneficially reduces blood glucose and cholesterol levels. Durian extract possesses anti-proliferative and probiotics effects in in vitro models. These effects warrant further investigation in human interventional studies for the development of functional food.
Tracing Primordial Magnetic Fields with 21 cm Line Observations
Authors: Kunze Kerstin E.
Published: March 2019

Magnetic fields are observed on a large range of scales in the universe. Up until recently, the evidence always pointed to magnetic fields associated with some kind of structure, from planets to clusters of galaxies. Blazar observations have been used to posit the first evidence of truly cosmological magnetic fields or void magnetic fields. A cosmological magnetic field generated in the very early universe before recombination has implications for the cosmic microwave background (CMB), large scale structure as well as the 21 cm line signal. In particular, the Lorentz term causes a change in the linear matter power spectrum. Its implication on the 21 cm line signal was the focus of our recent simulations which will be summarised here. Modelling the cosmological magnetic field as a gaussian random field numerical solutions were found for magnetic fields with present day amplitudes of 5 nG and negative spectral indices which are within the range of observational constraints imposed by the cosmic microwave background (CMB).
RNA-Seq Transcriptome Analysis of Rice Primary Roots Reveals the Role of Flavonoids in Regulating the Rice Primary Root Growth
Authors: Xu Yu , Zou Junjie , Zheng Hongyan , Xu Miaoyun , Zong Xuefeng , Wang Lei
Published: March 2019

Flavonoids play important roles in root development and in its tropic responses, whereas the flavonoids-mediated changes of the global transcription levels during root growth remain unclear. Here, the global transcription changes in quercetin-treated rice primary roots were analyzed. Quercetin treatment significantly induced the inhibition of root growth and the reduction of H2O2 and O2− levels. In addition, the RNA-seq analysis revealed that there are 1243 differentially expressed genes (DEGs) identified in quercetin-treated roots, including 1032 up-regulated and 211 down-regulated genes. A gene ontology (GO) enrichment analysis showed that the enriched GO terms are mainly associated with the cell wall organization, response to oxidative stress, and response to hormone stimulus. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis showed that the enriched DEGs are involved in phenylpropanoid biosynthesis, glutathione metabolism, and plant hormone signal transduction. Moreover, the quercetin treatment led to an increase of the antioxidant enzyme activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) in rice roots. Also, the quercetin treatment altered the DR5:GUS expression pattern in the root tips. All of these data indicated that the flavonoids-mediated transcription changes of genes are related to the genes involved in cell wall remodeling, redox homeostasis, and auxin signaling, leading to a reduced cell division in the meristem zone and cell elongation in the elongation zone of roots.
Genome-Wide Assessment of Avocado Germplasm Determined from Specific Length Amplified Fragment Sequencing and Transcriptomes: Population Structure, Genetic Diversity, Identification, and Application of Race-Specific Markers
Authors: Ge Yu , Zhang Teng , Wu Bin , Tan Lin , Ma Funing , Zou Minghong , Chen Haihong , Pei Jinli , Liu Yuanzheng , Chen Zhihao , Xu Zining , Wang Tao
Published: March 2019

Genomic data is a powerful tool. However, the phylogenetic relationships among different ecological races of avocado remain unclear. Here, we used the results from specific length amplified fragment sequencing (SLAF-seq) and transcriptome data to infer the population structure and genetic diversity of 21 avocado cultivars and reconstructed the phylogeny of three ecological races and two interracial hybrids. The results of the three analyses performed (unweighted pair-group methods with arithmetic means (UPGMA) cluster, Principal coordinate analysis (PCoA), and STRUCTURE) based on single nucleotide polymorphisms (SNPs) from SLAF-seq all indicated the existence of two populations based on botanical race: Mexican–Guatemalan and West Indian genotype populations. Our results based on SNPs from SLAF-seq indicated that the Mexican and Guatemalan races were more closely related to each other than either was to the West Indian race, which also was confirmed in the UPGMA cluster results based on SNPs from transcriptomic data. SNPs from SLAF-seq provided strong evidence that the Guatemalan, Mexican, and Guatemalan × Mexican hybrid accession possessed higher genetic diversity than the West Indian races and Guatemalan × West Indian hybrid accessions. Six race-specific Kompetitive allele specific PCR (KASP) markers based on SNPs from SLAF-seq were then developed and validated.
Quality Is in the Eye of the Beholder—A Focus Group Study from the Perspective of Ambulance Clinicians, Physicians, and Managers
Authors: Rantala Andreas , Behm Lina , Rosén Helena
Published: March 2019

Quality within all areas of healthcare should be systemically monitored and ensured. However, the definition of quality is complex and diverse. In the ambulance service (AS), quality has traditionally been defined as response time, but this measurement eliminates the possibility of addressing other characteristics of quality, such as the care provided. This study aimed to explore what constitutes quality in the context of the ambulance service as experienced by ambulance clinicians, physicians, and managers. A focus group study was conducted with 18 participants. The three focus groups were analyzed with the focus group method developed by Kreuger and Casey. The participants highlighted patient involvement, information and care, as well as adherence to policies, regulations, and their own standards as representing quality in the AS. This study demonstrates that quality is in the eye of the beholder. As quality seems to be viewed similarly by patients and ambulance clinicians, physicians, and managers, stakeholders should aim for a paradigm shift where patients’ experience of the care is just as important as various time measures.
Long Term Historic Changes in the Flow of Lesser Zab River, Iraq
Authors: Ali Rawshan , Ismael Arez , Heryansyah Arien , Nawaz Nadeem
Published: March 2019

The assessment of trends in river flows has become of interest to the scientific community in order to understand the changing characteristics of flow due to climate change. In this study, the trends in river flow of Dukan Dam located in the northern part of Iraq were assessed. The assessment was carried out for the period 1964 to 2013 using Sen’s slope and the Mann–Kendall test. Sen’s slope was used to assess the magnitude of change while the Mann–Kendall trend test was used to confirm the significance of trends. The results of the study showed that there was a decreasing trend in river flow both annually and for all individual months. The highest decreasing trend of −5.08846 m3/month was noticed in April, while the lowest change of −1.06022 m3/month was noticed in November. The annual flow also showed a significant decrease at a rate of −1.912 m3/year at a 95% level of confidence. Additionally, the findings of the study also confirmed that a decrease in precipitation and the construction of hydraulic structures reduced the flow in the river. The findings of the study suggest that decreasing trends may cause a water-scarce situation in the future if proper adaptation measures are not taken.
Dynamic Land Cover Mapping of Urbanized Cities with Landsat 8 Multi-temporal Images: Comparative Evaluation of Classification Algorithms and Dimension Reduction Methods
Authors: Alganci Ugur
Published: March 2019

Uncontrolled and continuous urbanization is an important problem in the metropolitan cities of developing countries. Urbanization progress that occurs due to population expansion and migration results in important changes in the land cover characteristics of a city. These changes mostly affect natural habitats and the ecosystem in a negative manner. Hence, urbanization-related changes should be monitored regularly, and land cover maps should be updated to reflect the current situation. This research presents a comparative evaluation of two classification algorithms, pixel-based support vector machine (SVM) classification and decision-tree-oriented geographic object-based image analysis (GEOBIA) classification, in producing a dynamic land cover map of the Istanbul metropolitan city in Turkey between 2013 and 2017 using Landsat 8 Operational Land Imager (OLI) multi-temporal satellite images. Additionally, the efficiencies of the two data dimension reduction methods are evaluated as part of this research. For dimension reduction, built-up index (BUI) and principal component analysis (PCA) data were calculated for five images during the mentioned period, and the classification algorithms were applied on data stacks for each dimension reduction method. The classification results indicate that the GEOBIA classification of the BUI data set provided the highest accuracy, with a 91.60% overall accuracy and 0.91 kappa value. This combination was followed by the GEOBIA classification of the PCA data set, which highlights the overall efficiency of the GEOBIA over the SVM method. On the other hand, the BUI data set provided more reliable and consistent results for urban expansion classes due to representing physical responses of the surface when compared to the data set of the PCA, which is a spectral transformation method.
Advances in Antigenic Peptide-Based Vaccine and Neutralizing Antibodies against Viruses Causing Hand, Foot, and Mouth Disease
Authors: Anasir Mohd Ishtiaq , Poh Chit Laa
Published: March 2019

Hand, foot, and mouth disease (HFMD) commonly produces herpangina, but fatal neurological complications have been observed in children. Enterovirus 71 (EV-A71) and Coxsackievirus 16 (CV-A16) are the predominant viruses causing HFMD worldwide. With rising concern about HFMD outbreaks, there is a need for an effective vaccine against EV-A71 and CV-A16. Although an inactivated vaccine has been developed against EV-A71 in China, the inability of the inactivated vaccine to confer protection against CV-A16 infection and other HFMD etiological agents, such as CV-A6 and CV-A10, necessitates the exploration of other vaccine platforms. Thus, the antigenic peptide-based vaccines are promising platforms to develop safe and efficacious multivalent vaccines, while the monoclonal antibodies are viable therapeutic and prophylactic agents against HFMD etiological agents. This article reviews the available information related to the antigenic peptides of the etiological agents of HFMD and their neutralizing antibodies that can provide a basis for the design of future therapies against HFMD etiological agents.
IL-36, IL-37, and IL-38 Cytokines in Skin and Joint Inflammation: A Comprehensive Review of Their Therapeutic Potential
Authors: Boutet Marie-Astrid , Nerviani Alessandra , Pitzalis Costantino
Published: March 2019

The interleukin (IL)-1 family of cytokines is composed of 11 members, including the most recently discovered IL-36α, β, γ, IL-37, and IL-38. Similar to IL-1, IL-36 cytokines are initiators and amplifiers of inflammation, whereas both IL-37 and IL-38 display anti-inflammatory activities. A few studies have outlined the role played by these cytokines in several inflammatory diseases. For instance, IL-36 agonists seem to be relevant for the pathogenesis of skin psoriasis whereas, despite being expressed within the synovial tissue, their silencing or overexpression do not critically influence the course of arthritis in mice. In this review, we will focus on the state of the art of the molecular features and biological roles of IL-36, IL-37, and IL-38 in representative skin- and joint-related inflammatory diseases, namely psoriasis, rheumatoid arthritis, and psoriatic arthritis. We will then offer an overview of the therapeutic potential of targeting the IL-36 axis in these diseases, either by blocking the proinflammatory agonists or enhancing the physiologic inhibitory feedback on the inflammation mediated by the antagonists IL-37 and IL-38.
Control of Silver Coating on Raman Label Incorporated Gold Nanoparticles Assembled Silica Nanoparticles
Authors: Pham Xuan-Hung , Hahm Eunil , Kang Eunji , Son Byung Sung , Ha Yuna , Kim Hyung-Mo , Jeong Dae Hong , Jun Bong-Hyun
Published: March 2019

Signal reproducibility in surface-enhanced Raman scattering (SERS) remains a challenge, limiting the scope of the quantitative applications of SERS. This drawback in quantitative SERS sensing can be overcome by incorporating internal standard chemicals between the core and shell structures of metal nanoparticles (NPs). Herein, we prepared a SERS-active core Raman labeling compound (RLC) shell material, based on Au–Ag NPs and assembled silica NPs (SiO2@Au@RLC@Ag NPs). Three types of RLCs were used as candidates for internal standards, including 4-mercaptobenzoic acid (4-MBA), 4-aminothiophenol (4-ATP) and 4-methylbenzenethiol (4-MBT), and their effects on the deposition of a silver shell were investigated. The formation of the Ag shell was strongly dependent on the concentration of the silver ion. The negative charge of SiO2@Au@RLCs facilitated the formation of an Ag shell. In various pH solutions, the size of the Ag NPs was larger at a low pH and smaller at a higher pH, due to a decrease in the reduction rate. The results provide a deeper understanding of features in silver deposition, to guide further research and development of a strong and reliable SERS probe based on SiO2@Au@RLC@Ag NPs.
Extracellular Vesicles as Diagnostics and Therapeutics for Structural Epilepsies
Authors: Karttunen Jenni , Heiskanen Mette , Lipponen Anssi , Poulsen David , Pitkänen Asla
Published: March 2019

Extracellular vesicles (EVs) are small vesicles involved in intercellular communication. Data is emerging that EVs and their cargo have potential as diagnostic biomarkers and treatments for brain diseases, including traumatic brain injury and epilepsy. Here, we summarize the current knowledge regarding changes in EV numbers and cargo in status epilepticus (SE) and traumatic brain injury (TBI), which are clinically significant etiologies for acquired epileptogenesis in animals and humans. We also review encouraging data, which suggests that EVs secreted by stem cells may serve as recovery-enhancing treatments for SE and TBI. Using Gene Set Enrichment Analysis, we show that brain EV-related transcripts are positively enriched in rodent models of epileptogenesis and epilepsy, and altered in response to anti-seizure drugs. These data suggest that EVs show promise as biomarkers, treatments and drug targets for epilepsy. In parallel to gathering conceptual knowledge, analytics platforms for the isolation and analysis of EV contents need to be further developed.
Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower
Authors: Horn Renate , Radanovic Aleksandra , Fuhrmann Lena , Sprycha Yves , Hamrit Sonia , Jockovic Milan , Miladinovic Dragana , Jansen Constantin
Published: March 2019

Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value < 10−4), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.
The Phosphoproteomic Response of Okra (Abelmoschus esculentus L.) Seedlings to Salt Stress
Authors: Yu Chenliang , Wu Qinqfei , Sun Chendong , Tang Mengling , Sun Junwei , Zhan Yihua
Published: March 2019

Soil salinization is a major environmental stresses that seriously threatens land use efficiency and crop yields worldwide. Although the overall response of plants to NaCl has been well studied, the contribution of protein phosphorylation to the detoxification and tolerance of NaCl in okra (Abelmoschus esculentus L.) seedlings is unclear. The molecular bases of okra seedlings’ responses to 300 mM NaCl stress are discussed in this study. Using a combination of affinity enrichment, tandem mass tag (TMT) labeling and high-performance liquid chromatography–tandem mass spectrometry analysis, a large-scale phosphoproteome analysis was performed in okra. A total of 4341 phosphorylation sites were identified on 2550 proteins, of which 3453 sites of 2268 proteins provided quantitative information. We found that 91 sites were upregulated and 307 sites were downregulated in the NaCl/control comparison group. Subsequently, we performed a systematic bioinformatics analysis including gene ontology annotation, domain annotation, subcellular localization, and Kyoto Encyclopedia of Genes and Genomes pathway annotation. The latter revealed that the differentially expressed proteins were most strongly associated with ‘photosynthesis antenna proteins’ and ‘RNA degradation’. These differentially expressed proteins probably play important roles in salt stress responses in okra. The results should help to increase our understanding of the molecular mechanisms of plant post-translational modifications in response to salt stress.
New Recombinant Cold-Adapted and Organic Solvent Tolerant Lipase from Psychrophilic Pseudomonas sp. LSK25, Isolated from Signy Island Antarctica
Authors: Salwoom Leelatulasi , Raja Abd. Rahman Raja Noor Zaliha , Salleh Abu Bakar , Mohd. Shariff Fairolniza , Convey Peter , Mohamad Ali Mohd Shukuri
Published: March 2019

In recent years, studies on psychrophilic lipases have become an emerging area of research in the field of enzymology. The study described here focuses on the cold-adapted organic solvent tolerant lipase strain Pseudomonas sp. LSK25 isolated from Signy Station, South Orkney Islands, maritime Antarctic. Strain LSK25 lipase was successfully cloned, sequenced, and over-expressed in an Escherichia coli system. Sequence analysis revealed that the lipase gene of Pseudomonas sp. LSK25 consists of 1432 bp, lacks an N-terminal signal peptide and encodes a mature protein consisting of 476 amino acids. The recombinant LSK25 lipase was purified by single-step purification using Ni-Sepharose affinity chromatography and had a molecular mass of approximately 65 kDa. The final recovery and purification fold were 44% and 1.3, respectively. The LSK25 lipase was optimally active at 30 °C and at pH 6. Stable lipolytic activity was reported between temperatures of 5–30 °C and at pH 6–8. A significant enhancement of lipolytic activity was observed in the presence of Ca2+ ions, the organic lipids of rice bran oil and coconut oil, a synthetic C12 ester and a wide range of water immiscible organic solvents. Overall, lipase strain LSK25 is a potentially desirable candidate for biotechnological application, due to its stability at low temperatures, across a range of pH and in organic solvents.
Suppressive Role of Androgen/Androgen Receptor Signaling via Chemokines on Prostate Cancer Cells
Authors: Izumi Kouji , Mizokami Atsushi
Published: March 2019

Androgen/androgen receptor (AR) signaling is a significant driver of prostate cancer progression, therefore androgen-deprivation therapy (ADT) is often used as a standard form of treatment for advanced and metastatic prostate cancer patients. However, after several years of ADT, prostate cancer progresses to castration-resistant prostate cancer (CRPC). Androgen/AR signaling is still considered an important factor for prostate cancer cell survival following CRPC progression, while recent studies have reported dichotomic roles for androgen/AR signaling. Androgen/AR signaling increases prostate cancer cell proliferation, while simultaneously inhibiting migration. As a result, ADT can induce prostate cancer metastasis. Several C-C motif ligand (CCL)-receptor (CCR) axes are involved in cancer cell migration related to blockade of androgen/AR signaling. The CCL2-CCR2 axis is negatively regulated by androgen/AR signaling, with the CCL22-CCR4 axis acting as a further downstream mediator, both of which promote prostate cancer cell migration. Furthermore, the CCL5-CCR5 axis inhibits androgen/AR signaling as an upstream mediator. CCL4 is involved in prostate carcinogenesis through macrophage AR signaling, while the CCL21-CCR7 axis in prostate cancer cells is activated by tumor necrotic factor, which is secreted when androgen/AR signaling is inhibited. Finally, the CCL2-CCR2 axis has recently been demonstrated to be a key contributor to cabazitaxel resistance in CRPC.
The Predictivity of N-Terminal Pro b-Type Natriuretic Peptide for All-Cause Mortality in Various Follow-Up Periods among Heart Failure Patients
Authors: Lai Min-Yu , Kan Wei-Chih , Huang Ya-Ting , Chen John , Shiao Chih-Chung
Published: March 2019

Plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) is an excellent prognostic–predictive tool in heart failure (HF) patients, but its plasma level changes following therapy. The comparison of prognosis–predictivity of a single measurement of plasma NT-pro BNP in different follow-up periods in acute HF patients has been less studied. This study aimed to evaluate whether the association between initial plasma NT-proBNP levels and all-cause mortality would decrease along with an increased follow-up period in patients with acute HF. The retrospective study was carried out, enrolling adult patients with hospitalization-requiring acute HF who fulfilled the predefined criteria from January 1, 2011, to December 31, 2013. We evaluated the independent predictors of 12-month mortality, and subsequently compared the predictivity of NT-proBNP level at initial presentation for 1-, 3-, 6-, 9- and 12-month mortality. In total, 269 patients (mean age, 74.45 ± 13.59 years; female, 53.9%) were enrolled. The independent predictors of 12-month mortality included higher “Charlson Comorbidity Index” (adjusted hazard ratio (aHR) = 1.22; 95% confidence interval (CI), 1.10–1.34), increased “age” (aHR = 1.07; 95% CI, 1.04–1.10), “administration of vasopressor” (aHR = 3.43; 95% CI, 1.76–6.71), “underwent cardiopulmonary resuscitation” (aHR = 4.59; 95% CI, 1.76–6.71), and without “angiotensin-converting enzyme inhibitors/angiotensin receptor blocker” (aHR = 0.41; 95% CI, 1.86–11.31) (all p <0.001). “Plasma NT-pro BNP level ≧11,755 ng/L” was demonstrated as an independent predictor in 1-month (aHR = 2.37; 95% CI, 1.10–5.11; p = 0.028) and 3-month mortality (aHR = 1.98; 95% CI, 1.02–3.86; p = 0.045) but not in more extended follow-up. The outcome predictivity of plasma NT-proBNP levels diminished in a longer follow-up period in hospitalized acute HF patients. In conclusion, these findings remind physicians to act with caution when using a single plasma level of NT-proBNP to predict patient outcomes with a longer follow-up period.
Systematic Review of Capnography with Mask Ventilation during Cardiopulmonary Resuscitation Maneuvers
Authors: Cereceda-Sánchez Francisco José , Molina-Mula Jesús
Published: March 2019

The latest guidelines identify capnography as an instrument used to assess bag-valve-mask ventilation during cardiopulmonary resuscitation (CPR). In this review, we analyzed the feasibility and reliability of capnography use with face mask ventilation during CPR maneuvers in adults and children. This systematic review was completed in December 2018; data for the study were obtained from the following databases: EBSCOhost, SCOPUS, PubMed, Índice Bibliográfico Español en Ciencias de la Salud (IBECS), TESEO, and Cochrane Library Plus. Two reviewers independently assessed the eligibility of the articles; we analyzed publications from different sources and identified studies that focused on the use of capnography with a face mask during CPR maneuvers in order to describe the capnometry value and its correlation with resuscitation outcomes and the assistance of professionals. A total of 888 papers were collected, and 17 papers were included that provided objective values for the use of capnography with a mask for ventilation. Four were randomized clinical trials (RCT) and the rest were observational studies. Four studies were completed in adults and 13 were completed in newborns. After the analysis of the papers, we recommended a capnographic level of C in adults and B in newborns. Despite the little evidence obtained, capnography has been demonstrated to facilitate the advanced clinical practice of mask ventilation in cardiopulmonary resuscitation, to be reliable in the early detection of heart rate increase in newborns, and to asses in-airway patency and lung aeration during newborn resuscitation.
Effects of Alkali Metal (Li, Na, and K) Incorporation in NH2–MIL125(Ti) on the Performance of CO2 Adsorption
Authors: Song Lifang , Xue Cheng , Xia Huiyun , Qiu Shujun , Sun Lixian , Chen Huaxin
Published: March 2019

A series of titanium-based, metal–organic framework (MOF) materials, xM@NH2-MIL125(Ti) (x is the alkali metal loading percentage during the synthesis; M = Li, Na, K), have been synthesized solvothermally. Alkali metal doping in the NH2–MIL125(Ti) in situ solvothermal process demonstrated a vital modification of the material structure and surface morphology for the CO2 adsorption capacity at ambient conditions. By changing the reactants’ precursor, including different kinds of alkali metal, the morphology of xM@NH2–MIL125(Ti) can be adjusted from a tetragonal plate through a circular plate to a truncated octahedron. The variation of the alkali metal loading results in substantial differences in the CO2 adsorption. The properties of xM@NH2–MIL125(Ti) were evaluated via functional group coordination using FT-IR, phase identification based on X-ray diffraction (XRD), surface morphology through scanning electron microscopy (SEM), as well as N2 and CO2 adsorption by physical gas adsorption analysis. This work reveals a new pathway to the modification of MOF materials for high-efficiency CO2 adsorption.
The Evaluation of the Fracture Surface in the AW-6060 T6 Aluminium Alloy under a Wide Range of Loads
Authors: Chybiński Marcin , Polus Łukasz , Ratajczak Maria , Sielicki Piotr W.
Published: March 2019

The present study focused on the behaviour of the AW-6060 aluminium alloy in peak temper condition T6 under a wide range of loads: tensile loading, projectile and explosion. The alloy is used as a structural component of civil engineering structures exposed to static or dynamic loads. Therefore, it was crucial to determine the material’s behaviour at low and intermediate rates of deformation. Despite the fact that the evaluation of the strain rate sensitivity of the AW-6060 aluminium alloy has already been discussed in literature, the authors of this paper wished to further investigate this topic. They conducted tensile tests and confirmed the thesis that the AW-6060 T6 aluminium alloy has low strain rate sensitivity at room temperature. In addition, the fracture surfaces subjected to different loading (tensile loading, projectile and explosion) were investigated and compared using a scanning electron microscope, because the authors of this paper were trying to develop a new methodology for predicting how samples had been loaded before failure occurred based on scanning electron microscopy (SEM) micrographs. Projectile and explosion tests were performed mainly for the SEM observation of the fracture surfaces. These tests were unconventional and they represent the originality of this research. It was found that the type of loading had an impact on the fracture surface.
Technological Platform for Vertical Multi-Wafer Integration of Microscanners and Micro-Optical Components
Authors: Bargiel Sylwester , Baranski Maciej , Wiemer Maik , Frömel Jörg , Wang Wei-Shan , Gorecki Christophe
Published: March 2019

We describe an original integration technological platform for the miniaturization of micromachined on-chip optical microscopes, such as the laser scanning confocal microscope. The platform employs the multi-wafer vertical integration approach, combined with integrated glass-based micro-optics as well as micro-electro-mechanical systems (MEMS) components, where the assembly uses the heterogeneous bonding and interconnecting technologies. Various heterogeneous components are disposed in vertically stacked building blocks (glass microlens, MEMS actuator, beamsplitter, etc.) in a minimum space. The platform offers the integrity and potential of MEMS microactuators integrated with micro-optics, providing miniaturized and low cost solutions to create micromachined on-chip optical microscopes.
Characterization a Novel Butyric Acid-Producing Bacterium Collinsella aerofaciens Subsp. Shenzhenensis Subsp. Nov.
Authors: Qin Panpan , Zou Yuanqiang , Dai Ying , Luo Guangwen , Zhang Xiaowei , Xiao Liang
Published: March 2019

Butyrate-producing bacteria can biosynthesize butyrate and alleviate inflammatory diseases. However, few studies have reported that the genus Collinsella has the ability to produce butyric acid. Here, our study depicts a Collinsella strain, which is a rod-shaped obligate anaerobe that is able to produce butyric acid. This microorganism was isolated from a human gut, and the optimal growth conditions were found to be 37 °C on PYG medium with pH 6.5. The 16S rRNA gene sequence demonstrated that this microorganism shared 99.93% similarity with C. aerofaciens ATCC 25986T, which was higher than the threshold (98.65%) for differentiating two species. Digital DNA–DNA hybridization and average nucleotide identity values also supported that this microorganism belonged to the species C. aerofaciens. Distinct phenotypic characteristics between TF06-26 and the type strain of C. aerofaciens, such as the fermentation of D-lactose, D-fructose and D-maltose, positive growth under pH 5 and 0.2% (w/v) cholate, suggested this strain was a novel subspecies. Comparative genome analysis revealed that butyric acid kinase and phosphate butyryltransferase enzymes were coded exclusively by this strain, indicating a specific butyric acid-producing function of this C. aerofaciens subspecies within the genus Collinsella. Thus, Collinsella aerofaciens subsp. shenzhenensis subsp. nov. was proposed, with set strain TF06-26T (=CGMCC 1.5216T = DSM 105138T) as the type strain.
The Geochemistry and Bioturbation of Clay Sediments Associated with Amalgamated Crusts at the Gagua Ridge
Authors: Chen Shuai , Zeng Zhigang , Wang Xiaoyuan , Yin Xuebo , Zhu Bowen , Guo Kun , Huang Xin
Published: March 2019

Based on the analysis of geochemical and mineralogical compositions, deep sea clay sediment characteristics and their material sources were examined in the eastern flank of the Gagua Ridge. The mineralogy mainly consists of detrital clay minerals, quartz, and authigenic phillipsite. There is scarce biogenic debris (siliceous or calcareous). The consolidated sediments are more enriched in Si, Al, K, Na, Li, Sc, Cr, Rb, and Cs than the associated crusts and nodules. The unmixed sediment samples were mainlycontributed by Asian eolian dust. The onset of the outer Fe-Mn crust growth nearly coincides with the Central Asia aridification event at ~3.5 Ma, which resulted in an abrupt increase in eolian flux of Asian dust. Intensified surface primary productivity is assumed to bring more metals to deep waters, and eventually facilitate the outer Fe-Mn crust formation. Authigenic phillipsite may come from the alteration of local basic volcanic glasses and cause excess Al, high Al/Ti, and low Si/Al ratios. However, phillipsites hardly affect the abundance of rare earth elements (REEs) and their patterns. In addition, the investigation of two kinds of burrows inside the consolidated sediments reveals that the inner nodules of the amalgamated crusts may remain on the oxic sediment surface, due to frequent benthic activities.