bluuebunny/crossref_metadata_2025 huggingface dataset:
The Spatial–Temporal Emission of Air Pollutants from Biomass Burning during Haze Episodes in Northern Thailand
Authors: Paluang Phakphum , Thavorntam Watinee , Phairuang Worradorn
Published: April 2024

Air pollutants from biomass burning, including forest fires and agricultural trash burning, have contributed significantly to the pollution of the Asian atmosphere. Burned area estimates are variable, making it difficult to measure these emissions. Improving emission quantification of these critical air pollution sources requires refining methods and collecting thorough data. This study estimates air pollutants from biomass burning, including PMs, NOX, SO2, BC, and OC. Machine learning (ML) with the Random Forest (RF) method was used to assess burned areas in Google Earth Engine. Forest emissions were highest in the upper north and peaked in March and April 2019. Air pollutants from agricultural waste residue were found in the lower north, but harvesting seasons made timing less reliable. Biomass burning was compared to the MODIS aerosol optical depth (AOD) and Sentinel-5P air pollutants, with all comparisons made by the Pollution Control Department (PCD) Thailand air monitoring stations. Agro-industries, mainly sugar factories, produce air pollutants by burning bagasse as biomass fuel. Meanwhile, the emission inventory of agricultural operations in northern Thailand, including that of agro-industry and forest fires, was found to have a good relationship with the monthly average levels of ambient air pollutants. Overall, the information uncovered in this study is vital for air quality control and mitigation in northern Thailand and elsewhere.
Severity, Logging and Microsite Influence Post-Fire Regeneration of Maritime Pine
Authors: Carrillo-García Cristina , Hernando Carmen , Díez Carmen , Guijarro Mercedes , Madrigal Javier
Published: April 2024

We investigated the influence of fire severity, logging of burnt wood, local ecological factors and their interaction on the natural regeneration, survival and growth of maritime pine (Pinus pinaster Ait.), following a fire that took place in 2005. During the period 2006–2020, a sample of 1900 seedlings were monitored, in which three post-fire treatments were applied: (1) Early logging (before seedling emergence); (2) Delayed logging (after emergence); and (3) No management. Multivariate semi-parametric and non-parametric techniques were used to model seedling survival, estimated density and growth of natural pine regeneration. Seedling survival was 31% with a mean density of more than 2000 seedlings/ha at the end of the study period. Logging before seedling emergence was positively related with pine survival and density. Delayed logging resulted in the lowest seedling density and regeneration. Fire severity had a negative influence on regeneration density. The findings indicate that site conditions and fire severity have a stronger influence on natural regeneration of maritime pine than subsequent post-fire management treatments. In order to ensure the presence of maritime pine in pure or mixed stands, silvicultural work is required to control competition from other species and reduce the risk of new wildfires.
Technological and Enzymatic Characterization of Autochthonous Lactic Acid Bacteria Isolated from Viili Natural Starters
Authors: Rampanti Giorgia , Cantarini Andrea , Cardinali Federica , Milanović Vesna , Garofalo Cristiana , Aquilanti Lucia , Osimani Andrea
Published: April 2024

Viili, a Finnish ropy fermented milk, is traditionally manufactured through spontaneous fermentation, by mesophilic lactic acid bacteria and yeast-like fungi, or back-slopping. This study evaluated four natural viili starters as sources of lactic acid bacteria for dairy production. Back-slopping activation of the studied viili samples was monitored through pH and titratable acidity measurements and enumeration of mesophilic lactic acid bacteria. Sixty lactic acid bacteria isolates were collected, molecularly identified, and assayed for acidification performance, enzymatic activities, production of exopolysaccharides (EPSs), presence of the histidine decarboxylase (hdcA) gene of Gram-positive bacteria, and production of bacteriocins. A neat predominance of Lactococcus lactis emerged among the isolates, followed by Enterococcus faecalis, Enterococcus faecium, Enterococcus durans, Enterococcus lactis, and Lactococcus cremoris. Most isolates exhibited proteolytic activity, whereas only a few enterococci showed lipase activity. Five isolates identified as L. cremoris, L. lactis, and E. faecalis showed a good acidification performance. Most of the isolates tested positive for leucine arylamidase, whereas only one E. durans and two L. lactis isolates were positive for valine arylamidase. A few isolates also showed a positive reaction for beta-galactosidase and alpha- and beta-glucosidase. None of the isolates produced EPSs or bacteriocins. The hdcA gene was detected in five isolates identified as L. lactis and E. faecium. A few L. cremoris and L. lactis isolates for potential use as starter or adjunct cultures for dairy processing were finally identified.
Physicochemical and Functional Properties of Citrullus mucosospermus, Citroides, and Moringa oleifera Seeds’ Hydrocolloids
Authors: Olubi Olakunbi , Obilana Anthony , Tshilumbu Nsenda , Fester Veruscha , Jideani Victoria
Published: April 2024

Hydrocolloids form gel-like structures when dispersed in water and have garnered significant attention for their diverse applications in food, pharmaceuticals, and other industries. The extraction of hydrocolloids from natural sources, such as seeds, presents an intriguing avenue due to the potential diversity in composition and functionality. Utilising seeds from Citrullus lanatus mucosospermus, lanatus citroides, and Moringa aligns with the growing demand for natural and sustainable ingredients in various industries. This research investigated hydrocolloids extracted from Citrullus mucosospermus (CMS), lanatus citroides, and Moringa oleifera seeds, highlighting their versatile physicochemical and functional attributes. Hydrocolloids were extracted from the seeds and subjected to analysis of their proximate composition, particle size distribution, and interfacial tension using the hot water extraction method. Protein content variation was observed among the raw oilseed (CMS, Citroides, and Moringa oleifera) flours. The protein content of the hydrocolloids surpassed that of raw oilseeds, significantly enhancing the amino acid profile. Furthermore, the hydrocolloid ash contents ranged from 4.09% to 6.52% w/w dry weight, coupled with low fat levels. The particle size distribution revealed predominantly fine particles with a narrow size distribution. All three hydrocolloids demonstrated remarkable oil- and water-holding capacities, highlighting their suitability for efficient stabilisation and emulsification in food formulations. These findings suggest the potential utilisation of these hydrocolloids as valuable ingredients across a spectrum of applications, encompassing food, pharmaceuticals, and industry, thus contributing to the development of sustainable and functional products. The unique attributes presented herein mark a noteworthy advancement in the understanding and application of novel hydrocolloids from CMS, Citroides, and Moringa oleifera.
Modeling and Predicting Passenger Load Factor in Air Transportation: A Deep Assessment Methodology with Fractional Calculus Approach Utilizing Reservation Data
Authors: Şimşek Kevser , Tuğrul Nisa Özge Önal , Karaçuha Kamil , Tabatadze Vasil , Karaçuha Ertuğrul
Published: April 2024

This study addresses the challenge of predicting the passenger load factor (PLF) in air transportation to optimize capacity management and revenue maximization. Leveraging historical reservation data from 19 Turkish Airlines market routes and sample flights, we propose a novel approach combining deep assessment methodology (DAM) with fractional calculus theory. By modeling the relationship between PLF and the number of days remaining until a flight, our method yields minimal errors compared to traditional techniques. Through a continuous curve constructed using the least-squares approach, we enable the anticipation of future flight values. Our analysis demonstrates that the DAM model with a first-order derivative outperforms linear techniques and the Fractional Model-3 in both modeling capabilities and prediction accuracy. The proposed approach offers a data-driven solution for efficiently managing air transport capacity, with implications for revenue optimization. Specifically, our modeling findings indicate that the DAM wd model improves prediction accuracy by approximately 0.67 times compared to the DAM model, surpassing the fractional model and regression analysis. For the DAM wd modeling method, the lowest average mean absolute percentage error (AMAPE) value achieved is 0.571, showcasing its effectiveness in forecasting flight outcomes.
Dynamic Fractional-Order Grey Prediction Model with GWO and MLP for Forecasting Overseas Talent Mobility in China
Authors: Wu Geng , Fu Haiwei , Jiang Peng , Chi Rui , Cai Rongjiang
Published: April 2024

International students play a crucial role in China’s talent development strategy. Thus, predicting overseas talent mobility is essential for formulating scientifically reasonable talent introduction policies, optimizing talent cultivation systems, and fostering international talent cooperation. In this study, we proposed a novel fractional-order grey model based on the Multi-Layer Perceptron (MLP) and Grey Wolf Optimizer (GWO) algorithm to forecast the movement of overseas talent, namely MGDFGM(1,1). Compared to the traditional grey model FGM(1,1), which utilizes the same fractional order at all time points, the proposed MGDFGM(1,1) model dynamically adjusts the fractional-order values based on the time point. This dynamic adjustment enables our model to better capture the changing trends in the data, thereby enhancing the model’s fitting capability. To validate the effectiveness of the MGDFGM(1,1) model, we primarily utilize Root Mean Square Error (RMSE) and Mean Absolute Percentage Error (MAPE) as the evaluation criteria for the prediction accuracy, as well as standard deviation (STD) as an indicator of the model stability. Furthermore, we perform experimental analysis to evaluate the predictive performance of the MGDFGM(1,1) model in comparison to NAÏVE, ARIMA, GM(1,1), FGM(1,1), LSSVR, MLP, and LSTM. The research findings demonstrate that the MGDFGM(1,1) model achieves a remarkably high level of prediction accuracy and stability for forecasting overseas talent mobility in China. The implications of this study offer valuable insights and assistance to government departments involved in overseas talent management.
Allele-Specific Regulation of the Candidate Autism Liability Gene RAI1 by the Enhancer Variant rs4925102 (C/G)
Authors: Yuan Xi , Chen Li , Saffen David
Published: April 2024

Retinoic acid-induced 1 (RAI1) is a dosage-sensitive gene that causes autistic phenotypes when deleted or duplicated. Observations from clinical cases and animal models also suggest that changes of RAI1 expression levels contribute to autism. Previously, we used a bioinformatic approach to identify several single nucleotide polymorphisms (SNPs) located within the 5′-region of RAI1 that correlate with RAI1 mRNA expression in the human brain. In particular, the SNP rs4925102 was identified as a candidate cis-acting regulatory variant, the genotype of which may affect the binding of transcription factors that influence RAI1 mRNA expression. In this study, we provide experimental evidence based on reporter gene, chromatin immunoprecipitation (ChIP), and chromatin conformation capture (3C) assays that rs4925102 regulates RAI1 mRNA expression in an allele-specific manner in human cell lines, including the neuroblastoma-derived cell line SH-SY5Y. We also describe a statistically significant association between rs4925102 genotype and autism spectrum disorder (ASD) diagnosis in a case-control study and near-statistically significant association in an Autism Genome Project (AGP) transmission disequilibrium (TDT) study using Caucasian subjects.
Clinical and Molecular Characterization of a Novel Homozygous Frameshift Variant in AEBP1-Related Classical-like Ehlers Danlos Syndrome Type 2 with Comparison to Previously Reported Rare Cases
Authors: Ha Zong Yi , Chijiwa Chieko , Lewis Suzanne
Published: April 2024

Recently, an autosomal recessive subtype of connective tissue disorder within the spectrum of Ehlers–Danlos syndrome (EDS), named classical-like EDS type 2 (clEDS2), was identified. clEDS2 is associated with biallelic variants in the adipocyte enhancer binding protein 1 (AEBP1) gene, specifically, affecting its aortic carboxypeptidase-like protein (ACLP) isoform. We described the 15th patient (13th family) diagnosed with clEDS2. This patient presented with notable similarities in phenotype to the documented cases, along with additional characteristics such as significant prematurity and short stature. An EDS sequencing panel-based analysis revealed homozygous AEBP1: NM_001129.5:c.2923del, p.Ala975Profs*22 likely pathogenic variants, and maternally inherited heterozygous COL11A1: NM_001854.4:c.1160A>G, p.Lys387Arg variant of uncertain significance in our patient. Upon comprehensive review of all previously reported clEDS2 patients, our patient exhibited the following overlapping phenotypes, including cutaneous features: hyperextensibility, atrophic scars/delayed wound healing (100%), easy bruising (100%), excessive skin (93%); skeletal features: generalized joint hypermobility (93%), pes planus (93%), dislocation/subluxation (93%); and cardiovascular features (86%). Our patient did not display symptoms of the critical complications reported in a few individuals, including superior mesenteric artery aneurysms and ruptures, aortic root aneurysm/dissection, spontaneous pneumothoraxes, and bowel ruptures. Together, this case expands the genetic and clinical phenotypic spectrum of AEBP1-related clEDS2.
Transcriptomic Changes and Regulatory Networks Associated with Resistance to Mastitis in Xinjiang Brown Cattle
Authors: Wang Dan , Yang Haiyan , Ma Shengchao , Liu Tingting , Yan Mengjie , Dong Mingming , Zhang Menghua , Zhang Tao , Zhang Xiaoxue , Xu Lei , Huang Xixia , Chen Hong
Published: April 2024

Xinjiang brown cattle are highly resistant to disease and tolerant of roughage feeding. The identification of genes regulating mastitis resistance in Xinjiang brown cattle is a novel means of genetic improvement. In this study, the blood levels of IL-1β, IL-6, IL-10, TNF-α, and TGF-β in Xinjiang brown cattle with high and low somatic cell counts (SCCs) were investigated, showing that cytokine levels were higher in cattle with high SCCs. The peripheral blood transcriptomic profiles of healthy and mastitis-affected cattle were constructed by RNA-seq. Differential expression analysis identified 1632 differentially expressed mRNAs (DE-mRNAs), 1757 differentially expressed lncRNAs (DE-lncRNAs), and 23 differentially expressed circRNAs (DE-circRNAs), which were found to be enriched in key pathways such as PI3K/Akt, focal adhesion, and ECM-receptor interactions. Finally, ceRNA interaction networks were constructed using the differentially expressed genes and ceRNAs. It was found that keynote genes or mRNAs were also enriched in pathways such as PI3K-Akt, cholinergic synapses, cell adhesion molecules, ion binding, cytokine receptor activity, and peptide receptor activity, suggesting that the key genes and ncRNAs in the network may play an important role in the regulation of bovine mastitis.
The Genome of Bifidobacterium longum subsp. infantis YLGB-1496 Provides Insights into Its Carbohydrate Utilization and Genetic Stability
Authors: Li Xiaoxia , Yang Jianjun , Shi Shaoqi , Lan Hanglian , Zhao Wen , Hung Weilian , He Jian , Wang Ran
Published: April 2024

Bifidobacterium longum subsp. infantis YLGB-1496 (YLGB-1496) is a probiotic strain isolated from human breast milk. The application of YLGB-1496 is influenced by carbohydrate utilization and genetic stability. This study used genome sequencing and morphology during continuous subculture to determine the carbohydrate utilization characteristics and genetic stability of YLGB-1496. The complete genome sequence of YLGB-1496 consists of 2,758,242 base pairs, 2442 coding sequences, and a GC content of 59.87%. A comparison of carbohydrate transport and metabolism genes of Bifidobacterium longum subsp. infantis (B. infantis) showed that YLGB-1496 was rich in glycosyl hydrolase 13, 20, 25, and 109 gene families. During continuous subculture, the growth characteristics and fermentation activity of the strain were highly stable. The bacterial cell surface and edges of the 1000th-generation strains were progressively smoother and well-defined, with no perforations or breaks in the cell wall. There were 20 SNP loci at the 1000th generation, fulfilling the requirement of belonging to the same strain. The presence of genes associated with cell adhesion and the absence of resistance genes supported the probiotic characteristics of the strain. The data obtained in this study provide insights into broad-spectrum carbohydrate utilization, genomic stability, and probiotic properties of YLGB-1496, which provide theoretical support to promote the use of YLGB-1496.
Redirect Tropism of Fowl Adenovirus 4 Vector by Modifying Fiber2 with Variable Domain of Heavy-Chain Antibody
Authors: Wang Yongjin , Zou Xiaohui , Guo Xiaojuan , Zhang Zhichao , Wang Min , Hung Tao , Lu Zhuozhuang
Published: April 2024

The variable domain of a heavy-chain antibody (VHH) has the potential to be used to redirect the cell tropism of adenoviral vectors. Here, we attempted to establish platforms to simplify the screening of VHHs for their specific targeting function when being incorporated into the fiber of adenovirus. Both fowl adenovirus 4 (FAdV-4) and simian adenovirus 1 (SAdV-1) have two types of fiber, one of which is dispensable for virus propagation and is a proper site for VHH display. An intermediate plasmid, pMD-FAV4Fs, was constructed as the start plasmid for FAdV-4 fiber2 modification. Foldon from phage T4 fibritin, a trigger for trimerization, was employed to bridge the tail/shaft domain of fiber2 and VHHs against human CD16A, a key membrane marker of natural killer (NK) cells. Through one step of restriction-assembly, the modified fiber2 was transferred to the adenoviral plasmid, which was linearized and transfected to packaging cells. Five FAdV-4 viruses carrying the GFP gene were finally rescued and amplified, with three VHHs being displayed. One recombinant virus, FAdV4FC21-EG, could hardly transduce human 293 or Jurkat cells. In contrast, when it was used at a multiplicity of infection of 1000 viral particles per cell, the transduction efficiency reached 51% or 34% for 293 or Jurkat cells expressing exogenous CD16A. Such a strategy of fiber modification was transplanted to the SAdV-1 vector to construct SAdV1FC28H-EG, which moderately transduced primary human NK cells while the parental virus transduced none. Collectively, we reformed the strategy of integrating VHH to fiber and established novel platforms for screening VHHs to construct adenoviral vectors with a specific tropism.
Effects of Telerehabilitation on Pain and Disability in Patients with Chronic Neck Pain: A Systematic Review and Meta-Analysis
Authors: Valenza-Peña Geraldine , Calvache-Mateo Andrés , Valenza Marie Carmen , Granados-Santiago María , Raya-Benítez Julia , Cabrera-Martos Irene , Díaz-Mohedo Esther
Published: April 2024

(1) Background: This systematic review and meta-analysis explores the effectiveness of telerehabilitation in patients suffering from chronic neck pain, specifically on pain and disability. The research delves into an area of growing significance within the realm of healthcare, aiming to understand the impact of digital interventions on the rehabilitation process for individuals with prolonged neck pain. (2) Methods: The comprehensive review encompasses a wide array of studies evaluating the collective outcomes of numerous trials focused on telerehabilitation strategies. In this systematic review, PubMed/MEDLINE, Scopus, and Web of Science databases were systematically searched to identify studies on telerehabilitation’s impact on pain. (3) Results: Eight studies met the inclusion criteria. Using the Downs and Black quality assessment, three studies were classified as good and five as fair. The authors identify specific modalities within telerehabilitation, such as remote exercise programs and virtual consultations, that contribute significantly to positive patient outcomes. Meta-analysis indicated a significant overall effect of telerehabilitation on pain reduction (MD = −1.27; 95% CI = −2.06; −0.47; p = 0.002). These findings support telerehabilitation’s efficacy in pain management. (4) Conclusions: The synthesis of evidence presented in this systematic review and meta-analysis underscores the potential of telerehabilitation as an effective and accessible means of managing chronic neck pain, offering valuable insights for both healthcare practitioners and policymakers in advancing patient-centered care.
Impact of Workplace Bullying on Quiet Quitting in Nurses: The Mediating Effect of Coping Strategies
Authors: Galanis Petros , Moisoglou Ioannis , Katsiroumpa Aglaia , Malliarou Maria , Vraka Irene , Gallos Parisis , Kalogeropoulou Maria , Papathanasiou Ioanna V.
Published: April 2024

Workplace bullying is common among nurses and negatively affects several work-related variables, such as job burnout and job satisfaction. However, no study until now has examined the impact of workplace bullying on quiet quitting among nurses. Thus, our aim was to examine the direct effect of workplace bullying on quiet quitting and to investigate the mediating effect of coping strategies on the relationship between workplace bullying and quiet quitting in nurses. We conducted a cross-sectional study with a convenience sample of 650 nurses in Greece. We collected our data in February 2024. We used the Negative Acts Questionnaire—Revised, the Quiet Quitting Scale, and the Brief COPE to measure workplace bullying, quiet quitting, and coping strategies, respectively. We found that workplace bullying and negative coping strategies were positive predictors of quiet quitting, while positive coping strategies were negative predictors of quiet quitting. Our mediation analysis showed that positive and negative coping strategies partially mediated the relationship between workplace bullying and quiet quitting. In particular, positive coping strategies caused competitive mediation, while negative coping strategies caused complimentary mediation. Nurses’ managers and policy makers should improve working conditions by reducing workplace bullying and strengthening positive coping strategies among nurses.
A Comparison of Veterans with Problematic Opioid Use Identified through Natural Language Processing of Clinical Notes versus Using Diagnostic Codes
Authors: Workman Terri Elizabeth , Kupersmith Joel , Ma Phillip , Spevak Christopher , Sandbrink Friedhelm , Cheng Yan , Zeng-Treitler Qing
Published: April 2024

Opioid use disorder is known to be under-coded as a diagnosis, yet problematic opioid use can be documented in clinical notes, which are included in electronic health records. We sought to identify problematic opioid use from a full range of clinical notes and compare the demographic and clinical characteristics of patients identified as having problematic opioid use exclusively in clinical notes to patients documented through ICD opioid use disorder diagnostic codes. We developed and applied a natural language processing (NLP) tool that combines rule-based pattern analysis and a trained support vector machine to the clinical notes of a patient cohort (n = 222,371) from two Veteran Affairs service regions to identify patients with problematic opioid use. We also used a set of ICD diagnostic codes to identify patients with opioid use disorder from the same cohort. The NLP tool achieved 96.6% specificity, 90.4% precision/PPV, 88.4% sensitivity/recall, and 94.4% accuracy on unseen test data. NLP exclusively identified 57,331 patients; 6997 patients had positive ICD code identifications. Patients exclusively identified through NLP were more likely to be women. Those identified through ICD codes were more likely to be male, younger, have concurrent benzodiazepine prescriptions, more comorbidities, and more care encounters, and were less likely to be married. Patients in both these groups had substantially elevated comorbidity levels compared with patients not documented through either method as experiencing problematic opioid use. Clinicians may be reluctant to code for opioid use disorder. It is therefore incumbent on the healthcare team to search for documentation of opioid concerns within clinical notes.
Experience of Older Patients with COPD Using Disease Management Apps: A Qualitative Study
Authors: Zou Xueqiong , Sun Pingping , Chen Mengjie , Nan Jiang , Gao Jing , Huang Xueying , Hou Yi , Jiang Yuyu
Published: April 2024

(1) Background: Digital medicine is developing in the management of chronic diseases in older people, but there is still a lack of information on the use of disease management apps in older patients with COPD. This study aims to explore the views and experience of older patients with COPD on disease management apps to provide a basis for the development and promotion of apps for geriatric diseases. (2) Methods: A descriptive qualitative research method was used. Older patients with COPD (N = 32) with experience using disease management apps participated in semi-structured interviews. Thematic analysis was used to analyze the data. (3) Results: Seven themes were defined: (a) feeling curious and worried when facing disease management apps for the first time; (b) actively overcoming barriers to use; (c) gradually becoming independent by continuous online learning; (d) feeling safe in the virtual environment; (e) gradually feeling new value in online interactions; (f) relying on disease management apps under long-term use; (g) expecting disease management apps to meet personalized needs. (4) Conclusions: The adoption and use of disease management apps by older people is a gradual process of acceptance, and they can obtain a wide range of benefits in health and life.
Knee Arthrodesis for Periprosthetic Knee Infection: Fusion Rate, Complications, and Limb Salvage—A Systematic Review
Authors: Mercurio Michele , Gasparini Giorgio , Cofano Erminia , Zappia Andrea , Familiari Filippo , Galasso Olimpio
Published: April 2024

The aim of this systematic review was to investigate the outcomes of knee arthrodesis (KA) after periprosthetic joint infection (PJI) of the knee. Differences in clinical outcomes and complication rates among the intramedullary nailing (IMN), external fixation (EF), and compression plating (CP) procedures were compared. A total of 23 studies were included. Demographics, microbiological data, types of implants, surgical techniques with complications, reoperations, fusion, and amputation rates were reported. A total of 787 patients were evaluated, of whom 601 (76.4%), 166 (21%), and 19 (2.4%) underwent IMN, EF, and CP, respectively. The most common causative pathogen was coagulase-negative Staphylococcus (CNS). Fusion occurred in 71.9%, 78.8%, and 92.3% of the patients after IMN, EF, and CP, respectively, and no statistically significant difference was found. Reinfection rates were 14.6%, 15.1%, and 10.5% after IMN, EF, and CP, respectively, and no statistically significant difference was found. Conversion to amputation occurred in 4.3%, 5%, and 15.8% of patients after IMN, EF, and CP, respectively; there was a higher rate after CP than after EF. The IMN technique is the most common option used for managing PJI with KA. No differences in terms of fusion, reinfection, or conversion-to-amputation rates were reported between IMN and EF. CP is rarely used, and the high amputation rate represents an important limitation of this technique.
Return to Work in Breast Cancer Patients following an Interdisciplinary Rehabilitation Program in a Community-Based Cancer Rehabilitation Center: A Pilot Study
Authors: Tay Matthew , Wong Chin , Aw Hui
Published: April 2024

Despite curative treatment and discharge from acute hospital settings, breast cancer patients often have cancer- and treatment-related morbidity which impairs them from returning to work. Hence, the role of community-based return to work rehabilitation programs is important to help these patients transition back to work. This was a retrospective cohort study involving patients with breast cancer conducted at a community-based cancer rehabilitation center. Patients were involved in an interdisciplinary vocational rehabilitation program involving physiatrists, occupational therapists, physiotherapists and social workers. We recruited 63 patients for this study cohort, with 46 (73.0%) patients ≤ 60 years old. After undergoing the rehabilitation program, there were 37 (58.7%) participants who successfully returned to work. These participants returned to work at either within 6 months (27.0%), 12 months (29.7%) or 24 months (43.2%) after enrollment into the program, with a majority enrolling in white collar jobs. Multivariate regression analysis revealed that significant negative factors for return to work were advanced stage of cancer (p = 0.004), along with clinically significant fatigue, measured on the Brief Fatigue Inventory (p < 0.001). However, perceived work ability (p = 0.020) was found to be a positive factor.
Integrated Metabolome and Transcriptome Analyses Reveal That the Flavonoid Metabolic Pathway Is Associated with Pigment Differential Accumulation in Two Colors of Petaloid Staminodes in Canna glauca
Authors: Zhao Tong , Wei Zehong , Liu Huanfang , Dong Limei
Published: April 2024

Canna glauca, an ornamental plant widely cultivated in aquatic habitats, is notable for its long florescence and showy flowers. The flower of this species is distinguished by its petaloid staminodes, which comprise the majority of the overall floral display. Flavonoids have been reported to be the predominant pigment groups that determine most flower colors. However, the influence of flavonoid metabolic pathways on the flower color of C. glauca remains to be investigated. In this study, comprehensive floral transcriptomes and metabolite profiles of the wild type (yellow flower) and ‘Erebus’ cultivar (pink flower) of C. glauca were analyzed. We identified 432 flavonoid metabolites, including 20 anthocyanins. ‘Erebus’ accumulated higher levels of 18 anthocyanins than the wild type, including 10 cyanidins, 4 pelargonidins, and 4 peonidins. The wild type accumulated higher levels of two malvidins. Through the joint analysis of transcriptomics and metabonomics, we observed a notable association between the expression of three DEGs and eleven anthocyanin levels. Furthermore, we analyzed the expression patterns of key genes that determine flavonoid biosynthesis, such as CHS, CHI, F3′H, and DFR. These findings provide enlightenment on the anthocyanin accumulation of Canna glauca, serving as a basis for exploring biochemical and molecular mechanisms underlying flower coloration.
Comparison of Different Grades of Innovative Tanyang Congou Black Tea (Minkehong) Based on Metabolomics and Sensory Evaluation
Authors: Zhou Zi-Wei , Wu Qing-Yang , Chen Li-Qin , Ruan Shu-Ling , Yang Zi-Yu , Sun Yun , Aikebaier Reheman
Published: April 2024

Innovative Tangyang Congou black tea, also known as Minkehong black tea (MKH), is of high quality and hence has gained a reputation on the market. In this study, standard samples of MKH were used as the research material, and the results of sensory evaluation showed that the overall quality of MKH decreased along with the downgrading. The radar chart showed a strong association between higher grades and specific aroma and taste character. Based on the detection of ultra-performance liquid chromatography–mass spectrometry (UPLC–MS) and related analysis, the content of both the ester-type catechins and non-ester catechins decreased along with the downgrading, and the details of their change trends were revealed. The content and dynamic changes in caffeine were also investigated. A total of 19 kinds of amino acids (AAs) were clustered; among them, 4 kinds of AAs, namely, Thr, Leu, Asp, and Ile, significantly contributed to the taste of the tea. Notably, the content of Thr had the highest correlation with the grade of MKH, and the correlation coefficient was 0.991 (p < 0.01). According to gas chromatography–mass spectrometry (GC–MS) detection and analysis, a total of 861 kinds of volatile compounds were detected, the aroma-active compounds in different grades were selected, and an aroma wheel of MKH was constructed. Our results found that non-volatile and volatile compounds not only contribute to defining the level of MKH standard samples but also provide a chemical basis for the measurement of flavour and quality of MKH.
Beyond Work: The Role of “Family-Friendly” Practices in the Subjective Well-Being of Teleworkers and On-Site Workers in the COVID-19 Pandemic
Authors: Lopes Sílvia , Couto Rita , Rodrigues Anabela , Sabino Ana , Oliveira Íris M. , Dias Paulo C. , Leite Ângela , Carvalho Vânia Sofia
Published: April 2024

During the COVID-19 pandemic, telework emerged as a pivotal strategy to mitigate the spread of the virus. However, telework’s feasibility was contingent on job roles. This gave rise to two distinct groups: teleworkers and on-site workers. However, the impacts of social support and well-being extended to both groups. This study investigated the link between organisational and supervisory family support and subjective well-being, examining work engagement as a mediator. Conducted in Portugal, this cross-sectional study surveyed 515 individuals via web-based questionnaires. Data were analysed using descriptive statistics, correlation analysis, confirmatory factor analysis, and multiple-group analysis. The findings revealed a positive correlation between perceived organisational family support (POFS) and work engagement for both groups. Additionally, perceived supervisory family support (PSFS) positively correlated with work engagement for telecommuters but not on-site workers. Furthermore, work engagement was positively associated with subjective well-being for both groups. Moreover, work engagement mediated the relationship between POFS and subjective well-being. This study enriches the literature by analysing POFS, PSFS, work engagement, and subjective well-being dynamics among teleworkers and on-site employees.
Acculturation and Health Status in the Children’s Healthy Living Program in the Pacific Region
Authors: Sparks Kalanikiekie S. , Fialkowski Marie K. , Dela Cruz Rica , Grandinetti Andrew , Wilkens Lynne , Banna Jinan C. , Bersamin Andrea , Paulino Yvette , Aflague Tanisha , Coleman Patricia , Deenik Jonathan , Fleming Travis , Novotny Rachel
Published: April 2024

Acculturation/enculturation has been found to impact childhood health and obesity status. The objective of this study is to use cross-sectional data to examine the association between proxies of adult/caregiver acculturation/enculturation and child health status (Body Mass Index [BMI], waist circumference [WC], and acanthosis nigricans [AN]) in the U.S.-Affiliated Pacific Islands (USAPI), Alaska, and Hawaiʻi. Study participants were from the Children’s Healthy Living (CHL) Program, an environmental intervention trial and obesity prevalence survey. Anthropometric data from 2–8 year olds and parent/caregiver questionnaires were used in this analysis. The results of this study (n = 4121) saw that those parents/caregivers who identified as traditional had children who were protected against overweight/obesity (OWOB) status and WC > 75th percentile (compared to the integrated culture identity) when adjusted for significant variables from the descriptive analysis. AN did not have a significant association with cultural classification. Future interventions in the USAPI, Alaska, and Hawaiʻi may want to focus efforts on parents/caregivers who associated with an integrated cultural group as an opportunity to improve health and reduce child OWOB prevalence.
More than Just a Number: Perspectives from Black Male Participants on Community-Based Interventions and Clinical Trials to Address Cardiovascular Health Disparities
Authors: Metlock Faith E. , Addison Sarah , McKoy Alicia , Yang Yesol , Hope Aarhea , Joseph Joshua J. , Zhang Jing , Williams Amaris , Gray Darrell M. , Gregory John , Nolan Timiya S.
Published: April 2024

Background: Black Americans remain significantly underrepresented and understudied in research. Community-based interventions have been increasingly recognized as an effective model for reckoning with clinical trial participation challenges amongst underrepresented groups, yet a paucity of studies implement this approach. The present study sought to gain insight into Black male participants’ perception of clinical trials before and after participating in a community-based team lifestyle intervention in the United States. Methods: Black Impact, a 24-week community-based lifestyle intervention, applied the American Heart Association’s Life’s Simple 7 (LS7) framework to assess changes in the cardiovascular health of seventy-four Black male participants partaking in weekly team-based physical activities and LS7-themed education and having their social needs addressed. A subset of twenty participants completed an exit survey via one of three semi-structured focus groups aimed at understanding the feasibility of interventions, including their perceptions of participating in clinical trials. Data were transcribed verbatim and analyzed using a content analysis, which involved systematically identifying, coding, categorizing, and interpreting the primary patterns of the data. Results: The participants reported a positive change in their perceptions of clinical trials based on their experience with a community-based lifestyle intervention. Three prominent themes regarding their perceptions of clinical trials prior to the intervention were as follows: (1) History of medical abuse; (2) Lack of diversity amongst research teams and participants; and (3) A positive experience with racially concordant research teams. Three themes noted to influence changes in their perception of clinical trials based on their participation in Black Impact were as follows: (1) Building trust with the research team; (2) Increasing awareness about clinical trials; and (3) Motivating participation through community engagement efforts. Conclusions: Improved perceptions of participating in clinical trials were achieved after participation in a community-based intervention. This intervention may provide a framework by which to facilitate clinical trial participation among Black men, which must be made a priority so that Black men are “more than just a number” and no longer “receiving the short end of the stick”.
Hemodynamic and Metabolic Responses to Moderate and Vigorous Cycle Ergometry in Men Who Have Had Transtibial Amputation
Authors: Storey Kionte K. , Geschwindt Adam , Astorino Todd A.
Published: April 2024

Adults who have had an amputation face barriers to having an active lifestyle which attenuates cardiorespiratory fitness. Prior studies in amputees typically involve treadmill walking or arm ergometry, yet physiological responses to bilateral leg cycling are less understood. This study assessed the hemodynamic and metabolic responses to moderate and vigorous cycle ergometry in men who have had a transtibial amputation (TTA). Five men who had had a unilateral TTA (age = 39 ± 15 yr) and six controls (CONs) without an amputation (age = 31 ± 11 yr) performed two 20 min bouts of cycling differing in intensity. Cardiac output (CO), stroke volume (SV), and oxygen consumption (VO2) were measured during moderate intensity continuous exercise (MICE) and high intensity interval exercise (HIIE) using thoracic impedance and indirect calorimetry. In response to MICE and HIIE, the HR and VO2 levels were similar (p > 0.05) between groups. Stroke volume and CO were higher (p < 0.05) in the CONs, which was attributed to their higher body mass. In men with TTAs, HIIE elicited a peak HR = 88%HRmax and substantial blood lactate accumulation, representing vigorous exercise intensity. No adverse events were exhibited in the men with TTAs. The men with TTAs show similar responses to MICE and HIIE versus the CONs.
Childhood Maltreatment and Adult Work Absenteeism: Work Meaningfulness as a Double-Edged Sword
Authors: Icekson Tamar , Kaye-Tzadok Avital , Zeiger Aya
Published: April 2024

The adverse impacts of childhood maltreatment (CM) on an individual’s health and economic welfare are widely recognized, yet its occupational and organizational effects remain less explored. Employee absenteeism, known as absenteeism, is often a sign of workplace maladjustment and may be linked to a history of CM. Some individuals in the helping professions, who exhibit a strong sense of purpose in their employment and pursue it in demanding environments, are CM survivors. This study investigates whether a heightened sense of meaningfulness in their work is associated with increased absenteeism among this subgroup. We recruited 320 helping professionals from a variety of social and mental health settings, one third of whom reported experiencing CM. As hypothesized, CM was positively correlated with work absenteeism. Furthermore, the relationship between work meaningfulness and absenteeism was moderated by their CM history: among those with CM experiences, greater work meaningfulness was associated with higher absenteeism rates. Our findings highlight the possibility that work meaningfulness may operate as a double-edged sword, and the importance of better understanding the challenges that high-functioning survivors of CM face within organizational contexts.
Combining the Antenatal Risk Questionnaire and the Edinburgh Postnatal Depression Scale in Early Pregnancy in Danish Antenatal Care—A Qualitative Descriptive Study
Authors: Broberg Lotte , Bendix Jane M. , Røhder Katrine , Løkkegaard Ellen , Væver Mette , Grew Julie C. , Johnsen Helle , Juhl Mette , de Lichtenberg Vibeke , Schiøtz Michaela
Published: April 2024

Pregnant women with a history of mental disorders, neglect, or low social support are at increased risk of mental health problems. It is crucial to identify psychosocial risk factors in early pregnancy to reduce the risk of short- and long-term health consequences for mother and child. The Antenatal Risk Questionnaire has been found acceptable as a psychosocial screening tool among pregnant women in Australia, but it has not been tested in a Scandinavian context. The aim of this study was to explore the experiences of pregnant women when using the Antenatal Risk Questionnaire and the Edinburgh Postnatal Depression Scale as part of a model to identify psychosocial vulnerabilities in pregnancy in Denmark. We conducted individual interviews (n = 18) and used thematic analysis. We identified two main themes: (1) Feeling heard and (2) An occasion for self-reflection. Overall, the pregnant women deemed the online ANRQ/EPDS acceptable as a screening tool. The screening model provided a feeling of being heard and provided an occasion for self-reflection about mental health challenges related to pregnancy and motherhood. However, some women expressed that the screening raised concerns and fear of the consequences of answering honestly. A non-judgmental, open, emphatic, and reassuring approach by clinicians may help reduce stigma.
A Novel Fibrin Matrix Derived from Platelet-Rich Plasma: Protocol and Characterization
Authors: Delgado Diego , Beitia Maider , Mercader Ruiz Jon , Sánchez Pello , Montoya-Alzola Marta , Fiz Nicolás , Sánchez Mikel
Published: April 2024

Although fibrin matrices derived from Platelet-Rich Plasma (PRP) are widely used in regenerative medicine, they have some limitations that can hinder their application. Modifying the composition of the PRP-derived fibrin matrix may improve its properties, making it suitable for certain medical uses. Three types of fibrin matrices were obtained: a PRP-derived fibrin matrix (FM), a PRP-derived fibrin matrix with a high fibrinogen content and platelets (FM-HFP) and a PRP-derived fibrin matrix with a high fibrinogen content (FM-HF). The fibrinogen levels, biomechanical properties and cell behavior were analyzed. The presence of platelets in the FM-HFP generated an inconsistent fibrin matrix that was discarded for the rest of the analysis. The fibrinogen levels in the FM-FH were higher than those in the FM (p < 0.0001), with a concentration factor of 6.86 ± 1.81. The values of clotting and swelling achieved using the FM-HF were higher (p < 0.0001), with less clot shrinkage (p < 0.0001). The FM had a significantly higher stiffness and turned out to be the most adherent composition (p = 0.027). In terms of cell viability, the FM-HF showed less cell proliferation but higher live/dead ratio values (p < 0.01). The increased fibrinogen and platelet removal in the FM-HF improved its adhesion and other biomechanical properties without affecting cell viability.
MGMT ProFWise: Unlocking a New Application for Combined Feature Selection and the Rank-Based Weighting Method to Link MGMT Methylation Status to Serum Protein Expression in Patients with Glioblastoma
Authors: Tasci Erdal , Shah Yajas , Jagasia Sarisha , Zhuge Ying , Shephard Jason , Johnson Margaret O. , Elemento Olivier , Joyce Thomas , Chappidi Shreya , Cooley Zgela Theresa , Sproull Mary , Mackey Megan , Camphausen Kevin , Krauze Andra Valentina
Published: April 2024

Glioblastoma (GBM) is a fatal brain tumor with limited treatment options. O6-methylguanine-DNA-methyltransferase (MGMT) promoter methylation status is the central molecular biomarker linked to both the response to temozolomide, the standard chemotherapy drug employed for GBM, and to patient survival. However, MGMT status is captured on tumor tissue which, given the difficulty in acquisition, limits the use of this molecular feature for treatment monitoring. MGMT protein expression levels may offer additional insights into the mechanistic understanding of MGMT but, currently, they correlate poorly to promoter methylation. The difficulty of acquiring tumor tissue for MGMT testing drives the need for non-invasive methods to predict MGMT status. Feature selection aims to identify the most informative features to build accurate and interpretable prediction models. This study explores the new application of a combined feature selection (i.e., LASSO and mRMR) and the rank-based weighting method (i.e., MGMT ProFWise) to non-invasively link MGMT promoter methylation status and serum protein expression in patients with GBM. Our method provides promising results, reducing dimensionality (by more than 95%) when employed on two large-scale proteomic datasets (7k SomaScan® panel and CPTAC) for all our analyses. The computational results indicate that the proposed approach provides 14 shared serum biomarkers that may be helpful for diagnostic, prognostic, and/or predictive operations for GBM-related processes, given further validation.