bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Clinically Implementable Digital PCR Assessments of DNA Methylation for Diagnosing Heavy Alcohol Consumption
Authors: Philibert Robert , Beach Steven R. H. , Andersen Allan M.
Published: December 2024

Background: Heavy alcohol consumption (HAC) has a profound adverse effect on human health. Unfortunately, there is a relative lack of tools that are easily implementable in clinical settings and that can be used to supplement self-reporting in the diagnosis and management of HAC. In part, this paucity is due to limitations of currently available biological measures and a mismatch between available biological measures and the needs of clinicians managing HAC. Objectives: We first review the pros and cons of existing biological measures. Next, we review the underlying theory and the performance characteristics of two recently developed methylation-sensitive digital PCR (MSdPCR) assays, referred to as the Alcohol T Score (ATS) and ZSCAN25, for the assessment of chronic and recent HAC, respectively. Finally, we outline a paradigm for improving the clinical diagnosis and management of alcohol use disorders by utilizing these new markers of alcohol consumption. Conclusions: We conclude that further studies to understand the test performance characteristics of each of these epigenetic tools in larger, diverse populations are in order.
Bark Biometry Along the Stem for Three Commercial Tree Species in Romania
Authors: Vasilescu Maria Magdalena
Published: December 2024

In general, bark serves a protective role for trees and is genetically determined. The quantification of bark based on biometric characteristics is linked to studies on the distribution of forest species across the globe and vegetation fires. In Romania, on the other hand, the improvement of the wood traceability system requires an increase in the accuracy of the estimation of the biometric characteristics of bark and, implicitly, of the volume of wood under the bark. The aim of this study was to develop more precise models for predicting bark thickness along the stem of three key Romanian species, taking into account a comprehensive range of models and stem sections, including those with a diameter over bark smaller than 8 cm, which have been excluded in previous studies. The study is based on two datasets, one containing the national measurements of three commercially valuable forest species, i.e., Norway spruce (Picea abies (L.) Karst), European beech (Fagus sylvatica L.), and pedunculate oak (Quercus robur L.) from 12,186 trees, and a second dataset containing the measurements from 61 logs of the same species at a specific forest site. A set of seven double bark thickness (DBT) estimation models with stem diameter over bark (DOB), DOB and total tree height (H), DOB and relative height along the stem (h/H), and diameter over bark at breast height (DBH) and DOB as predictors were used. The DBT models were evaluated using the coefficient of determination (R2), mean absolute error (MAE), root mean squared error (RMSE), the Akaike information criterion (AIC), and the Bayesian information criterion (BIC). This led to the selection of two more accurate models, Model 2 (based on a third-degree polynomial) and Model 3 (based on a logarithmic function), with DOB as the predictor. Relative double bark thickness (RDBT) and proportion of bark area (PBA) were also estimated using a sixth-degree polynomial and relative height as a predictor variable after stratifying the data by DBH classes to reduce variability. The results of this study indicate that there is a need to complete the database, for all three forest species of commercial value in Romania especially for large trees with DBH greater than 60–70 cm. The models obtained for PBA are of great use to the industry and the economy, in particular in the context of the traceability of wood. This is due to the fact that PBA can be equated with the proportion of bark volume (PBV), which describes the variation in the proportion of bark in the volume of the wood assortments along the stem. For a given DBH, PBA and PBV demonstrate minimal variability in sections from the tree’s base to a relative height of 0.6; however, a pronounced increase is observed at crown level in sections above relative heights of 0.8.
Composition and Diversity of Understory and Canopy Species Vary Along a Logging Gradient in an African Semi-Deciduous Tropical Rainforest
Authors: Kissa David Ocama , Nzunda Emmanuel Fred , Tweheyo Mnason , Lussetti Daniel , Ssekuubwa Enock , Sheil Douglas
Published: December 2024

The effect of timber cutting and related management on species composition and diversity in tropical forests has been reported in earlier studies, but the potentially different effects on understory and canopy tree species remains unclear. Our study aim was to assess the variation in species composition and diversity of understory and canopy species along a timber removal (“logging”) gradient. We assessed the species composition, alpha and beta diversity, and compared species composition of canopy trees in plots with different management histories in Budongo. Our findings revealed logging contributed 18.1% to the beta diversity of species composition as measured by distance-based redundancy analysis (dbRDA) and species composition decreased with logging intensity (R2 = −0.415). Unlogged forest had higher species diversity for both understory and canopy tree species compared with logged forests. Species composition of logged/unlogged forests were significantly different from those of the forest succession types. Our study adds new information on the effect of logging on the species composition of understory and canopy trees in lowland tropical forests. We found logged forests do not recover species composition within seven decades, diversity within, and what previously distinct successional types were, have become, and remain, mixed in nature.
Optimization of Time-Varying Temperature Profiles for Enhanced Beer Fermentation by Evolutive Algorithms
Authors: Ruarte Pablo , Pantano Nadia , Noriega Marianela , Fernández Cecilia , Serrano Emanuel , Scaglia Gustavo
Published: December 2024

Beer is one of the most popular alcoholic beverages globally, leading to continuous efforts to enhance its production methods. Raw materials and the production process are crucial in the brewing industry, with fermentation being a vital stage that significantly impacts beer quality. The aim of this study is to optimize the beer fermentation process by maximizing the ethanol concentration while minimizing species that adversely affect the organoleptic properties of beer. A novel optimization approach has been developed to derive an optimal, smooth, and continuous temperature profile that can be directly applied in real-world processes. This method integrates Fourier series and orthogonal polynomials for control action parameterization, in combination with evolutionary algorithms for parameter optimization. A key advantage of this methodology lies in its ability to handle a reduced parameter set efficiently, resulting in temperature profiles that are continuous and differentiable. This feature eliminates the need for post-smoothing and is particularly advantageous in biotechnological applications, where abrupt changes in temperature could negatively affect the viability of microorganisms. The optimized profiles not only enhance fermentation efficiency, but also improve the ethanol yield and reduce undesirable flavor compounds, providing a substantial improvement over current industrial practices. These advancements present significant potential for improving both the quality and consistency of beer production.
Development and Evaluation of Steel Component Coatings for Substations/Converter Stations with Both Fire and Corrosion Prevention Functions
Authors: Liu Yu , Chen Baohui , Wu Chuanping , Zhou Tiannian , Pan Bichen
Published: December 2024

There are a large number of steel components in substations/converter stations whose performance is seriously affected by being exposed to environmental corrosion and fire, endangering the operation of the substation/converter station. The current protective measures for steel components in substations/converter stations primarily involve the application of anti-corrosion and fireproof coatings. However, these coatings can easily peel off, resulting in a significant loss of their protective effectiveness. In response to this challenge, a new type of silicone-modified epoxy resin substrate has been synthesized by chemically grafting silicone resin onto epoxy resin segments, which retains the high adhesion of epoxy resin while enhancing its weather resistance. The use of synthesized nano zinc oxide-modified graphene oxide as a fireproof filler significantly improves the physical barrier effect and corrosion resistance of the coating. Additionally, the innovative addition of new metal anti-corrosion active pigments improves the adhesion and impermeability of the coating. Therefore, a steel structure coating for substations/converter stations with both fire and corrosion prevention functions has been developed. Standard tests conducted by national institutions have shown that the coating meets the performance requirements.
Revealing the Impact of Understory Fires on Stem Survival in Palms (Arecaceae): An Experimental Approach Using Predictive Models
Authors: Liesenfeld Marcus Vinicius de Athaydes
Published: December 2024

Amid increasing deforestation, surface fires reaching the forest understory are one of the primary threats to Amazonian ecosystems. Despite extensive research on post-fire mortality in woody species, the literature on palm resilience to fire is scant. This study investigates post-fire mortality in four understory palms, namely Bactris maraja Mart., Chamaedorea pauciflora Mart., Geonoma deversa (Poit.) Kunth, Hyospathe elegans Mart., and juvenile individuals of Euterpe precatoria Mart. (açaí palm). The objectives included (a) comparing post-fire responses; (b) developing mortality models based on severity variables; and (c) evaluating if diameter protects bud stems from heat flux. Conducted at the edge of an Ombrophylous Forest in Alto Juruá Acre, Brazil (7°45′ S, 72°22′ W), the experiment subjected 85 individuals to controlled burning in a 1 m2 area near the palm stem, with temperature sampling using K thermocouples. The results showed varying mortality rates among species, with a larger palm stem diameter correlating to reduced mortality. Crown burning patterns significantly influenced mortality, especially for Euterpe precatoria. The species exhibited diverse regrowth capacities, with B. maraja showing the highest number and tallest basal resprouts. The variation in morphology among species appeared to be more important than the amount of heat flux applied to each individual involved in the experiment, as no significant difference was observed in the time–temperature history measured. This study underscores post-fire plant mortality as a critical indicator of fire severity, essential for understanding its ecological impacts.
Lipoprotein Biology During Induced Oogenesis in the Shortfinned Eel, Anguilla australis—Vascular Transport
Authors: Damsteegt Erin L. , Mercuriali Benedetta , Thomson-Laing Georgia , Ward Joanna M. , Lokman P. Mark
Published: December 2024

The ecological, cultural and economic importance of freshwater eels, coupled with declining wild populations, drives the need for artificial propagation of these fish. Despite the closure of the lifecycle in captivity over a decade ago, numerous bottlenecks still prevent production at a commercially viable scale. Focusing on the key event of nutrient accumulation, we employed hypophysation over a 10-week period with biweekly sampling of female New Zealand shortfinned eels (Anguilla australis). Slotblots, colorimetric lipid/cholesterol assays, fast protein liquid chromatography and radioimmunoassay were used to assess aspects of blood chemistry. The lack of any trend in triglyceride and apolipoprotein B levels in plasma over time, combined with a significant reduction in cholesterol concentrations and a 10-fold increase in vitellogenin levels, confirmed a shift from low-density lipoprotein dominance—usually associated with lipid uptake and early oogenesis—to an abundance of high-density lipoproteins linked to vitellogenesis. The ongoing presence of 11-ketotestosterone in the circulation and the increase in 17β-estradiol levels reinforced the importance of steroids in mediating the gonadotropic signal associated with hypophysation. We conclude that the accumulation of both vitellogenin and apolipoprotein-B-associated lipids is essential for nutrient accumulation in eel oocytes during artificial induction of maturation.
Vegetable Oils and Their Use for Frying: A Review of Their Compositional Differences and Degradation
Authors: Abrante-Pascual Susana , Nieva-Echevarría Barbara , Goicoechea-Oses Encarnacion
Published: December 2024

This review provides an overview of the main vegetable oils of different botanical origin and composition that can be used for frying worldwide (olive and extra-virgin olive oil, high-oleic sunflower oil, rapeseed oil, peanut oil, rice bran oil, sunflower oil, corn oil, soybean oil, cottonseed oil, palm oil, palm kernel oil and coconut oil) and their degradation during this process. It is well known that during this culinary technique, oil’s major and minor components degrade throughout different reactions, mainly thermoxidation, polymerization and, to a lesser extent, hydrolysis. If severe high temperatures are employed, isomerization to trans fatty acyl chains and cyclization are also possible. The factors conditioning frying medium degradation are addressed, including oil composition (unsaturation degree, fatty acyl chain length and “free” fatty acid content, and presence of beneficial and detrimental minor components), together with frying conditions and food characteristics. Likewise, this review also tackles how the frying oil and other processing conditions may impact on fried food quality (oil absorption, texture, flavor and color). Finally, potential health implications of fried food consumption are briefly reviewed.
Development and Application of a Multiplex Reverse Transcription–Droplet Digital PCR Assay for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus in Bivalve Shellfish
Authors: Wei Maolin , Wang Jinfeng , Wang Yan , Liu Libing , Xu Xiangdong , Wang Jianchang
Published: December 2024

Foodborne viruses are significant contributors to global food safety incidents, posing a serious burden on human health and food safety. In this study, a multiplex reverse transcription–droplet digital PCR (RT-ddPCR) assay based on the MS2 phage as a process control virus (PCV) was developed to achieve the simultaneous detection of hepatitis A virus (HAV) and hepatitis E virus (HEV) in bivalve shellfish. By optimizing the reaction system and procedures, the best reaction conditions were selected, and the specificity, sensitivity, and reproducibility of the method were assessed. Additionally, the MS2 phage’s recovery rate was utilized as an indicator to evaluate the optimal sample nucleic acid enrichment method. The results indicated that the RT-ddPCR assay exhibited optimal amplification efficiency with primer concentrations of 900 nmol/L, probe concentrations of 350 nmol/L for HAV and HEV, and 500 nmol/L for MS2, an annealing temperature of 53.1 °C, an extension time of 90 s, and 45 cycles. Additionally, the developed multiplex RT-ddPCR assay demonstrated high specificity, with quantitation limits of 12.6, 8.9, and 7.8 copies/reaction being observed for HAV, HEV, and the MS2 phage, respectively. A total of 240 bivalve samples were analyzed, of which 4 were positive for HAV and 12 for HEV. The viral loads for HAV ranged from 3048 to 6528 copies/2 g, while those for HEV ranged from 3312 to 20,350 copies/2 g. This assay provides a vital tool for enhancing food safety monitoring.
Computer Vision-Based Fire–Ice Ion Algorithm for Rapid and Nondestructive Authentication of Ziziphi Spinosae Semen and Its Counterfeits
Authors: Chen Peng , Shao Xutong , Wen Guangyu , Song Yaowu , Fu Rao , Xiao Xiaoyan , Lu Tulin , Zhou Peina , Guo Qiaosheng , Shi Hongzhuan , Fei Chenghao
Published: December 2024

The authentication of Ziziphi Spinosae Semen (ZSS), Ziziphi Mauritianae Semen (ZMS), and Hovenia Acerba Semen (HAS) has become challenging. The chromatic and textural properties of ZSS, ZMS, and HAS are analyzed in this study. Color features were extracted via RGB, CIELAB, and HSI spaces, whereas texture information was analyzed via the gray-level co-occurrence matrix (GLCM) and Law’s texture feature analysis. The results revealed significant differences in color and texture among the samples. The fire–ice ion dimensionality reduction algorithm effectively fuses these features, enhancing their differentiation ability. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) confirmed the algorithm’s effectiveness, with variable importance in projection analysis (VIP analysis) (VIP > 1, p < 0.05) highlighting significant differences, particularly for the fire value, which is a key factor. To further validate the reliability of the algorithm, Back Propagation Neural Network (BP), Support Vector Machine (SVM), Deep Belief Network (DBN), and Random Forest (RF) were used for reverse validation, and the accuracy of the training set and test set reached 98.83–100% and 95.89–99.32%, respectively. The method provides a simple, low-cost, and high-precision tool for the fast and nondestructive detection of food authenticity.
Advances in Stellar and Galactic Evolution with the Population of Planetary Nebula Progenitors from the APOGEE DR17 Survey
Authors: Stanghellini Letizia , Smith Verne V. , Cunha Katia , Prantzos Nikos
Published: December 2024

Planetary nebulae (PNe) are the ejected gas and dust shells of evolved low- and intermediate-mass stars (LIMSs). We present an abundance comparison between PNe and their progenitors to reveal their similarities and differences since such a comparison has rarely, and not recently, been performed in the Milky Way. The dynamical expulsion of the outer envelope of an evolved LIMS produces the PN. We expected similarities in most α-element distributions across the stellar and nebular populations, given that these elements are only marginally produced and destroyed during the LIMS evolution. Differences found in the Fe and S abundances allow us to determine their depletion due to grain condensation in the post-AGB phases. Differences in N and C between PNe and their progenitors set new limits to the low- and intermediate-mass star contributions to these elements. Finally, radial metallicity gradients from evolved LIMS and PNe and Gaia-calibrated distances constrain Galactic evolution in the framework of the current chemical evolutionary models. We found the following: (1) Gas-phase iron is significantly depleted in PNe compared to their progenitor stars, with an average depletion factor of  = 1.74 ± 0.49. (2) Sulfur is also depleted in PNe, though to a much lesser extent than iron. (3) The median enrichment levels for carbon and nitrogen relative to the median stellar population of the same metallicity are approximately [C/H] ∼ +0.3 and [N/H] ∼ +0.4, respectively. PNe with progenitors that experienced hot-bottom burning (HBB) exhibit extreme nitrogen enrichment. (4) With the data available to date, the radial metallicity gradient derived from evolved LIMSs and PNe are compatible within the uncertainties.
Hydrate-Based Methane Storage in Biodegradable Hydrogels Absorbing Dilute Sodium P-Styrenesulfonate Solution
Authors: Hua Fangzheng , Tan Kang , Lv Jingyu , Wang Fei , Sun Mengting
Published: December 2024

Developing an exceptional reaction medium with high promotion efficiency, desirable biodegradability and good recyclability is necessary for hydrate-based methane storage. In this work, a kind of eco-friendly hydrogel, polyvinyl alcohol-co-acrylic acid (PVA-co-PAA), was utilized to absorb dilute sodium p-styrenesulfonate (SS) solution, for constructing a hybrid reaction medium for methane hydrate formation. Hydrogels or dilute SS solutions (1–4 mmol L−1) had weak or even no promoting effects on hydrate formation kinetics, while the combination of them could synergistically promote methane hydrate formation. In hydrogel-SS hybrid media containing 1, 2, 3 and 4 mmol L−1 of SS solutions, the storage capacity reached 121.2 ± 1.6, 121.5 ± 3.1, 122.6 ± 1.9 and 120.6 ± 1.6 v/v, respectively. In this binary reaction system, the large surface area of hydrogels provided hydrate formation with sufficient nucleation sites and an enlarged gas–liquid interface, and in the meantime, the dilute SS solution produced an adequate capillary effect, which together enhanced mass transfer and accelerated hydrate formation kinetics. Additionally, the hybrid medium could relieve wall-climbing hydrate growth and improve poor hydrate compactness resulting from the bulk SS promoter. Moreover, the hybrid medium exhibited a preferable recyclability and could be reused at least 10 times. Therefore, the hydrogel-SS hybrid medium can serve as an effective and eco-friendly packing medium for methane hydrate storage tanks, which holds great application potential in hydrate-based methane storage technology.
Construction of Promoter Elements for Strong, Moderate, and Weak Gene Expression in Drosophila melanogaster
Authors: Kudryashova Ksenia S. , Deriglazova Irina O. , Osadchiy Igor S. , Georgiev Pavel , Maksimenko Oksana
Published: December 2024

Background/Objectives: Transcriptional promoters play an essential role in regulating protein expression. Promoters with weak activity generally lead to low levels of expression, resulting in fewer proteins being produced. At the same time, strong promoters are commonly used in studies using transgenic organisms as model systems. This approach can have various negative consequences for the organism, as many regulatory proteins need to be expressed in small quantities, and excessive expression can have harmful effects on cells and organisms. Therefore, it is important to select the right promoter when creating transgenic organisms for research and practical applications. Methods: In this study, we used the Drosophila melanogaster genome as a source of natural promoter sequences for RNA polymerase II. These sequences were extracted and used to create a set of promoters that are suitable for practical application. The promoters were tested in a model system using fluorescent reporter genes in S2 cells and transgenic lines of Drosophila. Results: We assessed the expression levels of fluorescent reporter genes to rank the tested promoters from strongest to weakest. Six individual promoters of different sizes were established and compared. Additionally, we designed and tested three pairs of bidirectional promoters that could be used to simultaneously express two proteins. Conclusions: Based on our findings, we grouped the tested promoters into three categories: strong, moderate, and weak. These promoters can be utilized in transgenic model systems for protein production at different levels, from high to low. Bidirectional promoters, constructed “head-to-head”, meaning oppositely directed with the minimum distance between them, represent a novel tool for the co-expression of proteins.
Medieval Overexploitation of Peat Triggered Large-Scale Drowning and Permanent Land Loss in Coastal North Frisia (Wadden Sea Region, Germany)
Authors: Hadler Hanna , Reiß Antonia , Willershäuser Timo , Wilken Dennis , Blankenfeldt Ruth , Majchczack Bente , Klooß Stefanie , Ickerodt Ulf , Vött Andreas
Published: December 2024

Along the southern North Sea coast from the Netherlands to Denmark, human cultivation efforts have created a unique cultural landscape. Since the Middle Ages, these interactions between humans and natural forces have induced major coastal changes. In North Frisia (Germany), storm floods in 1362 AD and 1634 AD turned wide areas of embanked cultural land into tidal flats. Systematic geoarchaeological investigations between Nordstrand and Hallig Südfall comprise coring, trenching, sedimentary, geochemical and microfaunal palaeoenvironmental parameter analyses and radiocarbon dating. Together with geophysical prospection results and archaeological surveys, they give insights into the landscape’s development and causes for land losses. Results reveal that fens and bogs dominated from c. 800 BC to 1000 AD but are mostly missing in the stratigraphy. Instead, we found 12th to 14th cent. AD settlement remains directly on top of a pre-800 BC fossil marsh. This hiatus of c. 2000 years combined with local ‘Hufen’ settlements implies an extensive removal of peat during cultivation eventually resulting in the use of underlying marshland for agricultural purposes. Fifteenth cent. AD tidal flat deposits on top of the cultivated marsh prove that human impact lowered the ground surface below the mean high water of that time, clearly increasing the coastal vulnerability. We consider these intensive human–environment interactions as a decisive trigger for the massive loss of land and establishment of the tidal flats in North Frisia that are currently part of the UNESCO World Heritage “Wadden Sea”.
Job Demands and Resources Shape the Risk of Burnout in Italian Child Neuropsychiatrists
Authors: Raspanti Alessandra , Provenzi Livio , Acampora Marta , Borgatti Renato , Millepiedi Stefania , Mariani Wigley Isabella L. C. , Barello Serena
Published: December 2024

Objectives: To evaluate the influence of job demands and resources on burnout risk among Italian pediatric neuropsychiatrists. Methods: This cross-sectional study was conducted between December 2023 and February 2024 and involved Italian pediatric neuropsychiatrists. The study applied the Job Demands-Resources (JD-R) model to assess the impact of job demands (such as work–family conflict, time pressure, and job uncertainty) and job resources (like organizational support and perceived job meaning) on burnout. Burnout was measured through emotional exhaustion, depersonalization, and personal accomplishment subscales. Demographic data, including gender and career stage, were analyzed for their association with burnout. Results: High job demands were significantly associated with increased emotional exhaustion and depersonalization, while greater job resources correlated with lower burnout levels and higher personal accomplishment. Gender differences emerged, with female neuropsychiatrists reporting significantly higher emotional exhaustion and lower personal accomplishment than male neuropsychiatrists. Seniority was not a strong predictor, but early-career professionals, particularly residents, exhibited higher susceptibility to emotional exhaustion. Perceived job meaning and organizational support were protective factors across all burnout subscales, buffering the effects of job demands. Conclusions: Burnout risk in Italian pediatric neuropsychiatrists is shaped by both job demands and resources. Addressing work–family conflict, job uncertainty, and time pressure alongside enhancing organizational support and fostering job meaning is crucial to mitigate burnout. Special attention should be given to early-career professionals and female neuropsychiatrists to reduce their emotional exhaustion and improve their well-being. These findings provide valuable insights for developing targeted strategies to improve well-being in this field, ultimately enhancing patient care.
An Interdisciplinary Analysis of the Late Islamic Cemeteries Within the Cathedral (15th–18th Century CE) and the Ruins of Adulis (Mid-19th–Early 20th Century CE), Massawa, Eritrea: Funerary Architecture, Funerary Rituals, Burial Rites, and Bioarcheological Data Identifying Late Islamic Graves in Central Eastern Eritrea
Authors: Larentis Omar , Cattaneo Nelly , Lampugnani Paolo , Bortolotto Susanna , Zappa Emanuele , Gregorini Andrea , Gezae Yotam , Medin Tsegai , Gorini Ilaria , Massa Serena
Published: December 2024

Systematic studies on Late Islamic cemeteries that integrate architectural, ritual, and biological aspects remain relatively rare, particularly in Islamic countries or regions with an active Muslim presence. Typically, available research focuses more on epigraphic and artistic features. Since 2018, excavations at the Cathedral and surveys in the ruins of the city of Adulis (Massawa, Eritrea) have uncovered 326 Muslim graves: six dating to the 15th and 18th centuries CE and 320 attributed to the mid-19th to the early 20th century CE. These discoveries have enabled, for the first time in Eritrea, a detailed scientific study of the biological characteristics of the human remains, the funerary architecture, and the burial rites of Muslim communities from the 15th to the early 20th century. The skeletal remains were analyzed through taphonomic and anthropological studies. The graves were categorized into various typologies based on their structural features, while evidence of funerary rituals was documented as indicative elements of the burial practices adopted. These findings were further enriched by ethnographic sources and collaborative work with local communities, who acted as custodians of the historical memory and traditions of the region. The results provide insights into a historical period of Eritrea that is otherwise poorly documented. More broadly, they contribute to the understanding of the history of the Horn of Africa, a region with limited data from funerary and archaeological contexts. The Muslim cemeteries of Adulis represent the most extensively investigated Muslim burial site in recent years, providing critical insights into the historical Muslim presence in the region. These findings also allow researchers to hypothesize the succession of various communities that chose Adulis as a central location for interring their deceased, reflecting its enduring significance as a focal point for cultural and ritual practices across different periods.
Impact of Inquiry-Based Stress Reduction Intervention on Pharmacy Students’ Test Anxiety
Authors: Ndung’u Martha , Galal Suzanne , Tran Emily , Peng Matissa , Chang Jeffrey , Vyas Deepti , Mayberry John , Chima Avneet Kaur
Published: December 2024

Test anxiety is a significant issue among pharmacy students, potentially affecting their academic performance and well-being. This study aimed to evaluate the effectiveness of an Inquiry-Based Stress Reduction (IBSR) intervention in reducing test anxiety. Pharmacy students enrolled in a didactic course were randomized into an IBSR intervention group or a control group. Both groups attended a 1.5 h seminar; the intervention group learned the IBSR technique, while the control group received instruction on an unrelated topic (emotional intelligence). The intervention group also submitted weekly self-reflections on applying IBSR to exam-related anxiety. Anxiety levels were measured using the State-Trait Anxiety Inventory (STAI) at the start of two major exams. Descriptive data were collected and STAI results were analyzed via independent t-tests. Among the 86 participants, the average baseline STAI score indicated moderate–severe anxiety (51/80). No significant differences were found in anxiety scores between the groups before exam 2 (p = 0.99) and exam 3 (p = 0.60). However, 67% of the intervention group found IBSR helpful, 58% changed their test-taking approach, and 61% altered their self-perception. While IBSR did not significantly reduce pre-exam anxiety levels, it was perceived as beneficial by many students. Future research should explore additional strategies for managing test anxiety in this population.
Techno-Economic Feasibility of Fuel Cell Vehicle-to-Grid Fast Frequency Control in Non-Interconnected Islands
Authors: Christos Tziotas , Pompodakis Evangelos E. , Orfanoudakis Georgios I.
Published: December 2024

This paper presents an innovative approach to fast frequency control in electric grids by leveraging parked fuel cell electric vehicles (FCEVs), especially heavy-duty vehicles such as trucks. Equipped with hydrogen storage tanks and fuel cells, these vehicles can be repurposed as dynamic grid-support assets while parked in designated areas. Using an external cable and inverter system, FCEVs inject power into the grid by converting DC from fuel cells into AC, to be compatible with grid requirements. This functionality addresses sudden power imbalances, providing a rapid and efficient solution for frequency stabilization. The system’s external inverter serves as a central control hub, monitoring real-time grid frequency and directing FCEVs to supply virtual inertia and primary reserves through droop control, as required. Simulation results validate that FCEVs could effectively complement thermal generators, preventing unacceptable frequency drops, load shedding, and network blackouts. A techno-economic analysis demonstrates the economic feasibility of the concept, concluding that each FCEV consumes approximately 0.3 kg of hydrogen per day, incurring a daily cost of around EUR 1.5. For an island grid with a nominal power of 100 MW, maintaining frequency stability requires a fleet of 100 FCEVs, resulting in a total daily cost of EUR 150. Compared to a grid-scale battery system offering equivalent frequency response services, the proposed solution is up to three times more cost-effective, highlighting its economic and technical potential for grid stabilization in renewable-rich, non-interconnected power systems.
Climate Change and Health: A Study of the Attitudes of Future Science Teachers
Authors: Pérez-Mesa María Rocío , Porras-Contreras Yair Alexander , Tuay-Sigua Rosa Nidia
Published: December 2024

Living beings as open systems depend on climate and weather to survive. However, changes in the Earth’s climatology, which have become more frequent since the industrial period, have affected different territories of the planet, limiting access to ecosystem services and causing imbalances in health and well-being. The first purpose of this study is to conduct a literature review on academic production regarding climate change and its impact on health, in the context of education, using international academic production condensed in the Web of Science (WOS) database over the last 10 years as a reference. The second purpose focuses on identifying the environmental attitudes of science teachers in initial training regarding aspects related to climate change. The study results show three categories emerging from the literature review: Climate Change and Health, Nature and Risks, and Environment and Energy. For the analysis of environmental attitudes, a survey was conducted with 51 pre-service teachers, consisting of 59 items distributed in five categories: (a) environment, (b) climate change, (c) health, (d) education, and (e) lifestyle. Although the results reveal a positive attitude towards all analyzed categories, it is important to advance effective mitigation and adaptation strategies from the teacher training processes themselves.
Regional-Scale Equidistance Optimizing Method Considering the Equidistance Patterns of Discrete Global Grid Systems
Authors: Luo Fuli , Wang Lei , Yu Yue , Cui Tengfei , Han Li
Published: December 2024

The Discrete Global Grid System (DGGS) provides a foundational framework for the digital Earth, where uniform intercell distances are essential for accurate numerical simulations. However, due to the spherical topology, achieving strictly equidistant spherical grid cells is impractical. Most existing studies have focused on regional scales, which are constrained by data acquisition limitations and render global equidistant optimization algorithms economically infeasible. The equidistant characteristics of cells are influenced by map projections and often exhibit regional variations. In this paper, we analyze these equidistant characteristics and construct an equidistant pattern for an icosahedral hexagonal DGGS. By integrating this pattern into the icosahedral orientation method, we develop a regional-scale equidistant optimization method for DGGS. Experiments on river network extraction in the Yangtze River Basin demonstrate significant improvements: the equidistance of grid cells covering the region increased by over 34.2%, while the accuracy of river network extraction improved by 51.41%. Moreover, this method is extensible to other grid models, enhancing the broader applicability of DGGS.
SCOBY Cellulose-Based Materials Hydrophobized Using Stearic Acid and Apple Powder
Authors: Bryszewska Malgorzata Anita , Pareja Daniel Gutierez , Kaczmarek Lukasz , Sobczyk-Guzenda Anna , Piotrowska Malgorzata , Batory Damian
Published: December 2024

Bacterial cellulose (BC) is a subject of interest for researchers due to its advantageous characteristics, including a straightforward manufacturing process, biocompatibility, and extensive modification potential. The hydrophilic nature of the material is beneficial in some applications, yet a limiting factor in others. This study aimed to develop BC-based materials with goFogureod moisture resistance. The modification of bacterial cellulose (BC) using apple powder, stearic acid, or a combination of these modifiers resulted in the formation of a range of materials, some of which had their surfaces additionally functionalised by coating with a mixture of apple powder and stearic acid (HSt). The nature and type of changes were confirmed by FTIR and theoretical analysis, which was conducted by modelling the interaction between cellulose and homogalacturonan or rhamnogalacturonan using SCIGRESS v.FJ 2.7 software. Changes in hydrogen bonding resulting in a weakening of the interactions between cellulose and water in the presence of pectin were demonstrated by both empirical data and modelling. The effectiveness of BC functionalisation was confirmed by material wettability. The water contact angle changed from 38° for the unmodified material to 125° for the material obtained by modification of the bacterial cellulose with glycerol followed by modification with a mixture of HSt at a concentration of 10% and AP at a concentration of 60%. The modifications produced a material with a robust hydrophobic surface. The results suggest that the surface roughness may not be the primary factor influencing the hydrophilicity or hydrophobicity of these materials but that it is more likely to be related to the interactions of components. None of the tested materials demonstrated antimicrobial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Aspergillus niger, or Candida albicans.
The Potential of Naturally Derived Compounds for Treating Chronic Kidney Disease: A Review of Autophagy and Cellular Senescence
Authors: Teh Yoong Mond , Mualif Siti Aisyah , Mohd Noh Nur Izzati , Lim Soo Kun
Published: December 2024

Chronic kidney disease (CKD) is characterized by irreversible progressive worsening of kidney function leading to kidney failure. CKD is viewed as a clinical model of premature aging and to date, there is no treatment to reverse kidney damage. The well-established treatment for CKD aims to control factors that may aggravate kidney progression and to provide kidney protection effects to delay the progression of kidney disease. As an alternative, Traditional Chinese Medicine (TCM) has been shown to have fewer adverse effects for CKD patients. However, there is a lack of clinical and molecular studies investigating the mechanisms by which natural products used in TCM can improve CKD. In recent years, autophagy and cellular senescence have been identified as key contributors to aging and age-related diseases. Exploring the potential of natural products in TCM to target these processes in CKD patients could slow disease progression. A better understanding of the characteristics of these natural products and their effects on autophagy and cellular senescence through clinical studies, coupled with the use of these products as complementary therapy alongside mainstream treatment, may maximize therapeutic benefits and minimize adverse effects for CKD patients. While promising, there is currently a lack of thorough research on the potential synergistic effects of these natural products. This review examines the use of natural products in TCM as an alternative treatment for CKD and discusses their active ingredients in terms of renoprotection, autophagy, and cellular senescence.
In Vitro Toxicity of a DEHP and Cadmium Mixture on Sheep Cumulus–Oocyte Complexes
Authors: Mastrorocco Antonella , Temerario Letizia , Vurchio Valeria , Cotecchia Susanna , Martino Nicola Antonio , Dell’Aquila Maria Elena
Published: December 2024

Di-(2-ethylhexyl) phthalate (DEHP) and Cadmium (Cd) affect female reproduction. To date, toxicological research has focused on the effects of individual contaminants, whereas living beings are exposed to mixtures. This study analyzed the effects of a DEHP/Cd mixture on nuclear and cytoplasmic maturation of sheep cumulus–oocyte complexes (COCs) compared with single compounds. COCs recovered from slaughterhouses-derived sheep ovaries were in vitro exposed to 0.5 μM DEHP, 0.1 μM Cd, or DEHP/Cd mixture at the same concentrations during 24 h of in vitro maturation (IVM). After IVM, oocyte nuclear chromatin configuration was evaluated, and bioenergetic/oxidative parameters were assessed on expanded cumulus cells (CCs) and matured oocytes (chi-square test and one-way ANOVA; p < 0.05). Under examined conditions, oocyte nuclear maturation was never impaired. However, COC bioenergetics was affected with stronger effects for the mixture than single compounds. Indeed, the percentages of matured oocytes with healthy mitochondrial distribution patterns were reduced (p < 0.001 and p < 0.05 for mixture and single compounds, respectively). Oocyte mitochondrial membrane potential, intracellular ROS levels, and mitochondria/ROS co-localization were reduced, with the same significance level, in all contaminated conditions. CCs displayed increased ROS levels only upon mixture exposure (p < 0.001). In conclusion, in vitro exposure to the DEHP/Cd mixture affected COC quality in the sheep to a greater extent than separate compounds.
Neutrophil and Colorectal Cancer
Authors: Masui Hideyuki , Kawada Kenji , Obama Kazutaka
Published: December 2024

Colorectal cancer (CRC) is often associated with metastasis and recurrence and is the leading cause of cancer-related mortality. In the progression of CRC, recent studies have highlighted the critical role of neutrophils, particularly tumor-associated neutrophils (TANs). TANs have both tumor-promoting and tumor-suppressing activities, contributing to metastasis, immunosuppression, angiogenesis, and epithelial-to-mesenchymal transition. Tumor-promoting TANs promote tumor growth by releasing proteases, reactive oxygen species, and cytokines, whereas tumor-suppressing TANs enhance immune responses by activating T cells and natural killer cells. Understanding the mechanisms underlying TAN mobilization, plasticity, and their role in the tumor microenvironment has revealed potential therapeutic targets. This review provides a comprehensive overview of TAN biology in CRC and discusses both the tumor-promoting and tumor-suppressing functions of neutrophils. Novel therapeutic approaches targeting TANs, such as chemokine receptor antagonists, aim to modulate neutrophil reprogramming and offer promising avenues for improving treatment outcomes of CRC.
Pro-Health Potential of Fruit Vinegars and Oxymels in Various Experimental Models
Authors: Olas Beata
Published: December 2024

Fruits are excellent sources of substrate for various fermented products, including fruit vinegars, which are typically produced by submerged fermentation. Some evidence suggests that fruit vinegar consumption can alleviate certain disorders, including hyperlipidemia, inflammation, and hyperglycemia. Fruit vinegars also have bacteriostatic and antihypertensive actions. Recent studies also suggest that apple vinegar may offer benefits in treating insulin resistance, osteoporosis, and certain neurological diseases such as Alzheimer’s disease; it may also support weight loss. Recent studies in animal and human models have considerably broadened our understanding of the biological properties of not only fruit vinegars but also oxymels, i.e., mixtures of vinegar and honey or sugar. This paper reviews the current state of knowledge regarding vinegars and oxymels, with a special emphasis on their chemical composition and the mechanisms behind their biological activity and pro-health potential. The multidirectional effects of fruit vinegars and oxymels result from the synergy of different chemical compounds, including organic acids (mainly acetic acid), phenolic compounds, vitamins, minerals, and fermentation products. However, more studies are needed to understand the interactions between all the different components, not only the phenolic compounds and organic acids. In addition, more research is needed on their mechanisms of action. Although no serious side effects have been noted to date, further studies with large sample sizes are needed to understand the possible side effects of long-term fruit vinegar and oxymel use.
The Combination of Phage Therapy and β-Lactam Antibiotics for the Effective Treatment of Enterococcus faecalis Infections
Authors: Moryl Magdalena , Szychowska Paulina , Dziąg Julia , Różalski Antoni , Torzewska Agnieszka
Published: December 2024

A phage–antibiotic synergy could be an alternative in urinary tract infection (UTI) therapy, as it leads to the elimination of bacteria and to the reduction in variants resistant to phages and antibiotics. The aims of the in vitro study were to determine whether phages vB_Efa29212_2e and vB_Efa29212_3e interact synergistically with selected antibiotics in the treatment of E. faecalis infections, to optimize antibiotic concentrations and phage titers for the most effective combinations, and to assess their impact on the number of spontaneous resistant variants and on the phages’ reproductive cycles. The modified double-layer disc diffusion method, checkboard, time–kill assays, one-step growth method and the double agar overlay plaque assay were implemented. Synergistic interactions were most often observed after the combined action of phages 2e or 3e and β-lactam antibiotics on E. faecalis strains. The beneficial effects depended on the bacterial strain, phage and antibiotic used. The lowest minimum inhibitory concentration (MIC50) values of the antibiotics were recorded, after the application of low titers of phage 2e, and high titers of phage 3e. The combined use of the tested agents resulted in a significant reduction in the number of resistant variants and had an impact on the reproductive cycle of the tested phages, e.g., a 50% increase in burst size, and a 5 min reduction in the latency period of 2e were observed. The study confirmed beneficial interactions between phages and β-lactam antibiotics against E. faecalis growth.