bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Selected Meteorological Factors on the Growth Rate and Seed Yield of Winter Wheat—A Case Study
Authors: Węgrzyn Alicja , Klimek-Kopyra Agnieszka , Dacewicz Ewa , Skowera Barbara , Grygierzec Wiesław , Kulig Bogdan , Flis-Olszewska Ewelina
Published: November 2022

Recent years have seen a significant increase in weather anomalies in Central Europe (51°22′ N, 22°64′ E), which makes it more difficult to predict yields of winter wheat and to estimate prices on world exchanges. Eight-year (2007–2015) field observations of winter wheat cv. Turnia were carried out in order to determine the relationship between selected meteorological indicators (e.g., the values of average daily air temperature, daily sums of precipitation, and the number of days with precipitation) and the biometric traits and seed yield of winter wheat. In addition, an attempt was made to identify the meteorological parameters that determine an increase in stem elongation during the growing season. The relationship between yield parameters and biometric parameters was evaluated using Spearman’s non-parametric correlation coefficients. The regression models for analysis of the dependence of yield parameters and biometric parameters on meteorological elements (temperature, precipitation totals, and days with precipitation) in each development period was used. The study showed that from the beginning of vegetative growth to the flowering stage, growth and development were most advanced in plants for which the average temperature was about 8–9 °C, and the number of days with precipitation was about 41. During generative growth, spike length is 80% determined by the precipitation total. Grain weight is 68% negatively determined by the temperature during dormancy (the higher the temperature during dormancy, the lower the weight of the kernel). A positive relationship was shown between yield and the precipitation total during the period from sowing to the onset of dormancy (r = 0.37). Winter wheat yield was 69% dependent on the density at harvest (the greater the density, the higher the final yield). On the basis of the 8-year results of the study, we conclude that the weather is highly variable from year to year, and therefore breeders should direct their research towards creating variants with much greater plasticity and high tolerance for unfavourable weather patterns.
Semantic Segmentation of Wheat Stripe Rust Images Using Deep Learning
Authors: Li Yang , Qiao Tianle , Leng Wenbo , Jiao Wenrui , Luo Jing , Lv Yang , Tong Yiran , Mei Xuanjing , Li Hongsheng , Hu Qiongqiong , Yao Qiang
Published: November 2022

Wheat stripe rust-damaged leaves present challenges to automatic disease index calculation, including high similarity between spores and spots, and difficulty in distinguishing edge contours. In actual field applications, investigators rely on the naked eye to judge the disease extent, which is subjective, of low accuracy, and essentially qualitative. To address the above issues, this study undertook a task of semantic segmentation of wheat stripe rust damage images using deep learning. To address the problem of small available datasets, the first large-scale open dataset of wheat stripe rust images from Qinghai province was constructed through field and greenhouse image acquisition, screening, filtering, and manual annotation. There were 33,238 images in our dataset with a size of 512 × 512 pixels. A new segmentation paradigm was defined. Dividing indistinguishable spores and spots into different classes, the task of accurate segmentation of the background, leaf (containing spots), and spores was investigated. To assign different weights to high- and low-frequency features, we used the Octave-UNet model that replaces the original convolutional operation with the octave convolution in the U-Net model. The Octave-UNet model obtained the best benchmark results among four models (PSPNet, DeepLabv3, U-Net, Octave-UNet), the mean intersection over a union of the Octave-UNet model was 83.44%, the mean pixel accuracy was 94.58%, and the accuracy was 96.06%, respectively. The results showed that the state-of-art Octave-UNet model can better represent and discern the semantic information over a small region and improve the segmentation accuracy of spores, leaves, and backgrounds in our constructed dataset.
Fine Points of Marker-Assisted Pyramiding of Anthocyanin Biosynthesis Regulatory Genes for the Creation of Black-Grained Bread Wheat (Triticum aestivum L.) Lines
Authors: Gordeeva Elena , Shoeva Olesya , Mursalimov Sergey , Adonina Irina , Khlestkina Elena
Published: November 2022

Enrichment of grains with anthocyanins is considered a feasible approach to improving the nutritional properties of bread wheat. Here, two black-grained substitution lines with either 4B or 4D chromosomes substituted by wheatgrass (Thinopyrum ponticum) 4Th were created via marker-assisted combining the anthocyanin biosynthesis regulatory genes Pp-1, Pp3 (Purple pericarp), and Ba1 (Blue aleurone) in the genetics background of cv. Saratovskaya 29. The black grains manifested the simultaneous accumulation of anthocyanins in aleurone, and the pericarp layers resulted in the highest total anthocyanin content (TAC), which amounted to approximately the sum of TACs of the purple and blue grains. The lines with substitution 4Th(4B) had long trichomes on the leaves, whereas the lines with substitution 4Th(4D) did not differ from parental cv. Saratovskaya 29. Despite the chromosome substitution having a positive effect on the main spike length, the other yield-related traits (grain weight per main spike, the spike number per plant, and the 1000-grain weight) were decreased in the lines with chromosome substitution. The developed lines together with the parental ones constitute a valuable model for comparative studies and genetic stock for breeding commercial wheat cultivars featuring high levels of anthocyanins in grains.
Fish Microbiome Modulation and Convenient Storage of Aquafeeds When Supplemented with Vitamin K1
Authors: Acosta Marcos , Quiroz Eduardo , Tovar-Ramírez Dariel , Roberto Vânia Palma , Dias Jorge , Gavaia Paulo J. , Fernández Ignacio
Published: November 2022

Vitamin K (VK) is a fat-soluble vitamin necessary for fish metabolism and health. VK stability as dietary component during aquafeed storage and its potential effect on intestinal microbiome in fish have not yet been completely elucidated. The convenient storage conditions of aquafeeds when supplemented with phylloquinone (VK1), as well as its potential effects on the gut microbiota of Senegalese sole (Solea senegalensis) juveniles, have been explored. Experimental feeds were formulated to contain 0, 250 and 1250 mg kg−1 of VK1 and were stored at different temperatures (4, −20 or −80 °C). VK stability was superior at −20 °C for short-term (7 days) storage, while storing at −80 °C was best suited for long-term storage (up to 3 months). A comparison of bacterial communities from Senegalese sole fed diets containing 0 or 1250 mg kg−1 of VK1 showed that VK1 supplementation decreased the abundance of the Vibrio, Pseudoalteromonas, and Rhodobacterace families. All these microorganisms were previously associated with poor health status in aquatic organisms. These results contribute not only to a greater understanding of the physiological effects of vitamin K, particularly through fish intestinal microbiome, but also establish practical guidelines in the industry for proper aquafeed storage when supplemented with VK1.
Issues Regarding the Welfare of Assistance Dogs
Authors: Salmon Amanda , Driscoll Carlie , Paterson Mandy B. A. , Harpur Paul , Pachana Nancy A.
Published: November 2022

While the roles and efficacy of assistance animals have received attention in the literature, there has been less research focused on animal welfare issues regarding assistance animals. This is a pertinent area, given the burgeoning of types of assistance animals, situations in which they are employed, and access issues arising from increased assistance animal engagement. Animal welfare as pertains to assistance animals is discussed in this paper with respect to overall research on animal welfare concerns in a variety of contexts, training and access issues, and legal and regulatory concerns. Relevant examples from global contexts, as well as the specific Australian context, are offered. Conclusions include that while human quality of life is often considered and protected in laws and policies, this is much less true for assistance animals. Additional attention is required to ensure that the quality of life of both persons with disabilities and their assistance animals can be ensured. Support for a person to meet the needs of an assistance animal, as well as considerations for keeping such working dyads together in changed circumstances (e.g., following a move to an assisted living mode of accommodation), are recommended.
One-Step Laparoscopic Abomasopexy for Left Displacements of the Abomasum in Pregnant Cattle: A Retrospective Study
Authors: Iso Hideo , Uchiyama Fumikazu , Tsuka Takeshi
Published: November 2022

Although technical descriptions have been published, the applicability of left-flank laparoscopy for the surgical correction of left displacement of the abomasum (LDA) in pregnant cattle has not yet been fully assessed. This study aimed to investigate the postoperative performance of one-step laparoscopic abomasopexy for the correction of LDA during late pregnancy. Fifteen pregnant Holstein cattle with LDA were treated with one-step laparoscopic abomasopexy between days 259 and 274 of gestation. This technique was performed in a standing position. Under endoscopic observation via trocars introduced from the left flank, the animals underwent a surgical procedure to place the bar part of a toggle–pin–suture (TPS) device into the lumen of the abomasum through a long cannula. A suture of a TPS device was secured to the ventral abdominal wall by using a long applicator. No cases experienced postoperative complications, and all had a normal delivery on postoperative day 17.4 ± 4.9. Three of the 15 animals exhibited foot diseases and mastitis after calving. The reproductive performance was recorded during lactation periods, showing a service conception rate of 2.9 ± 1.2 and 110.5 ± 39.1 open days. The one-year survival rate was 60% (9/15). The positive postoperative results demonstrate the benefits of one-step laparoscopic abomasopexy in pregnant bovine cases with LDA.
Evaluation of Effectiveness and Safety of Microcin C7 in Weaned Piglets
Authors: Shang Lijun , Zhou Junyan , Tu Jiayu , Zeng Xiangfang , Qiao Shiyan
Published: November 2022

The effects and safety of dietary supplementation with Microcin C7 (C7) were evaluated in 216 weaned piglets. The pigs were given a control corn–soybean meal basal diet or C7 diet (control diet supplemented with 250, 500, 750, 1000, or 5000 mg C7/kg diets). Compared with the control group, the 500 mg/kg C7 supplementation group had better intestinal morphological indicators (p < 0.05), which may help maintain intestinal epithelial function and increase the growth performance (p < 0.05) and apparent total tract digestibility (p < 0.05). The diarrhea indexes of the 250, 500, and 750 mg/kg groups were significantly lower than that of the control group at 0–28 d (p < 0.05), and the 500 mg/kg group had the lowest diarrhea indexes (linear and quadratic, p < 0.05). A comprehensive analysis showed that microbial structure was significantly correlated with the degree of diarrhea, and the diarrhea-alleviating effect of C7 may be related to its selective regulation of specific microbial taxa. The 250 and 500 mg/kg C7 supplementation also significantly improved several immune indices of piglets (p < 0.05). Compared with the control diet, 5000 mg/kg C7 supplementation had no significant adverse effect on all parameters. Overall, the 250–500 mg/kg dose had the best effect, and the highest dose (5000 mg/kg) posed no toxicity risk. Therefore, C7 appears safe for use as an alternative to antibiotic growth promoters in weaned piglets.
Asymmetric Male Mating Success in Lek-Breeding Rhinella arenarum
Authors: Sinsch Ulrich , Hecht Katharina , Kost Silvia , Grenat Pablo R. , Martino Adolfo L.
Published: November 2022

Mate choice is the attempt of an individual to gain higher reproductive fitness by preferring to mate with some individuals and not with others. We studied the role of mate choice in the mating system of the neotropical toad Rhinella arenarum by assessing male reproductive tactics for mate acquisition and the contribution of female choice for pair formation. In a shallow pond in central Argentina, we estimated male mating success and the corresponding reproductive tactics by focal observation. The variation of phenotypic and genotypic traits (size and shape, longevity, vocalization features, heterozygosity) was related to the observed mating success in 110 males. The phonotactic response of 21 reproductive females to conspecific advertisement call features was tested in arena experiments. Mating success was limited to 32 males, pair formation was size-assortative. The dominant reproductive tactics were advertising from call positions near suitable breeding sites and pre-mating fights with intruding males, whereas the interception of amplectant pairs and the displacement of mated males were never observed. Female phonotaxis was directed to conspecific choruses but complex and simple call structures were not distinguished. We conclude that the mating system is a lek combining pre-mating fights among males and female choice of slightly smaller males. Fights interfere with female choice, undermining size-assortative mating. This is a unique system in the R. marina species group, in which interception behavior dominates reproduction.
Locomotory Profiles in Thoroughbreds: Peak Stride Length and Frequency in Training and Association with Race Outcomes
Authors: Schrurs Charlotte , Blott Sarah , Dubois Guillaume , Van Erck-Westergren Emmanuelle , Gardner David S.
Published: November 2022

Racehorses competing in short (i.e., ‘sprinters’), middle- or longer-distance (i.e., ‘stayers’) flat races are assumed to have natural variation in locomotion; sprinters having an innately shorter stride than stayers. No study has objectively tested this theory. Here, racehorses (n = 421) were categorised as sprinters, milers or stayers based on known race distance (n = 3269 races). Stride parameters (peak length and frequency) of those racehorses were collected from prior race-pace training sessions on turf (n = 2689; ‘jumpout’, n = 1013), using a locomotion monitoring device. Pedigree information for all 421 racehorses was extracted to three-generations. In training, sprinters had a shorter stride of higher frequency and covered consecutive furlongs faster than stayers (p < 0.001). Relatively short or longer stride did not predict race success, but stayers had greater race success than sprinters (p < 0.001). Peak stride length and frequency were moderately heritable (h2 = 0.15 and 0.20, respectively). In conclusion, differences in stride were apparent between sprinters and stayers (e.g., shorter stride in sprinters) during routine training, even after accounting for their pedigree. Objective data on stride characteristics could supplement other less objectively obtained parameters to benefit trainers in the appropriate selection of races for each individual racehorse.
Blocking TRPV4 Ameliorates Osteoarthritis by Inhibiting M1 Macrophage Polarization via the ROS/NLRP3 Signaling Pathway
Authors: Sun Heng , Sun Ziying , Xu Xingquan , Lv Zhongyang , Li Jiawei , Wu Rui , Fei Yuxiang , Tan Guihua , Liu Zizheng , Liu Yuan , Shi Dongquan
Published: November 2022

Osteoarthritis (OA) is a low-level inflammatory disease in which synovial macrophage M1 polarization exacerbates the progression of synovitis and OA. Notedly, the ROS (reactive oxygen species) level in macrophages is intimately implicated in macrophage M1 polarization. TRPV4 (transient receptor potential channel subfamily V member 4), as an ion channel, plays a pivotal role in oxidative stress and inflammation. In this study, we investigated the role of TRPV4 in OA progression and M1 macrophage polarization. Male adult Sprague–Dawley (SD) rats underwent a medial meniscus radial transection operation to create an OA model in vivo and RAW 264.7 cells were intervened with 100 ng/mL LPS (lipopolysaccharide) to induce M1-polarized macrophages in vitro. We demonstrated that the infiltration of M1 synovial macrophages and the expression of TRPV4 were increased significantly in OA synovium. In addition, intra-articular injection of HC067074 (a specific inhibitor of TRPV4) alleviated the progression of rat OA and significantly decreased synovial macrophage M1 polarization. Further mechanisms suggested that ROS production by M1 macrophages was decreased after TRPV4 inhibition. In addition, NLRP3 (pyrin domain containing protein 3) as a downstream effector of ROS in M1-polarized macrophage, was significantly suppressed following TRPV4 inhibition. In conclusion, this study discovered that inhibition of TRPV4 delays OA progression by inhibiting M1 synovial macrophage polarization through the ROS/NLRP3 pathway.
L-Citrulline Supplementation Reduces Blood Pressure and Myocardial Infarct Size under Chronic Intermittent Hypoxia, a Major Feature of Sleep Apnea Syndrome
Authors: Ozcan Bilgehan , Blachot-Minassian Britanny , Paradis Stéphanie , Mazière Lucile , Chambion-Diaz Marie , Bouyon Sophie , Pépin Jean-Louis , Pialoux Vincent , Arnaud Claire , Moinard Christophe , Belaidi Elise
Published: November 2022

Intermittent hypoxia (IH) is a landmark of obstructive sleep apnea (OSA) at the core of the cardiovascular consequences of OSA. IH triggers oxidative stress, a major underlying mechanism for elevated blood pressure (BP) and increased infarct size. L-citrulline is an amino acid that has been demonstrated to be protective of the cardiovascular system and exert pleiotropic effects. Therefore, we tested the impact of citrulline supplementation on IH-induced increase in BP and infarct size. Four groups of rats exposed to normoxia (N) or IH [14 days (d), 8 h/day, 30 s-O2 21%/30 s-O2 5%] and were supplemented or not with citrulline (1 g·kg−1·d−1). After 14 d, BP was measured, and hearts were submitted to global ischemia-reperfusion to measure infarct size. Histological and biochemical analyses were conducted on hearts and aorta to assess oxidative stress. Citrulline significantly reduced BP (–9.92%) and infarct size (–18.22%) under IH only. In the aorta, citrulline supplementation significantly decreased superoxide anion and nitrotyrosine levels under IH and abolished the IH-induced decrease in nitrite. Citrulline supplementation significantly decreased myocardial superoxide anion levels and xanthine oxidase enzyme activity under IH. Citrulline shows a cardioprotective capacity by limiting IH-induced pro-oxidant activity. Our results suggest that citrulline might represent a new pharmacological strategy in OSA patients with high cardiovascular risk.
ME-YOLO: Improved YOLOv5 for Detecting Medical Personal Protective Equipment
Authors: Wu Baizheng , Pang Chengxin , Zeng Xinhua , Hu Xing
Published: November 2022

Corona Virus Disease 2019 (COVID-19) poses a significant threat to human health and safety. As the core of the prevention and control of COVID-19, the health and safety of medical and nursing personnel are extremely important, and the standardized use of medical personal protective equipment can effectively prevent cross-infection. Due to the existence of severe occlusion and overlap, traditional image processing methods struggle to meet the demand for real-time detection. To address these problems, we propose the ME-YOLO model, which is an improved model based on the one-stage detector. To improve the feature extraction ability of the backbone network, we propose a feature fusion module (FFM) merged with the C3 module, named C3_FFM. To fully retain the semantic information and global features of the up-sampled feature map, we propose an up-sampling enhancement module (USEM). Furthermore, to achieve high-accuracy localization, we use EIoU as the loss function of the border regression. The experimental results demonstrate that ME-YOLO can better balance performance (97.2% mAP) and efficiency (53 FPS), meeting the requirements of real-time detection.
Efficient Knowledge Distillation for Brain Tumor Segmentation
Authors: Qi Yuan , Zhang Wenyin , Wang Xing , You Xinya , Hu Shunbo , Chen Ji
Published: November 2022

Deep learning has allowed great progress to be made in obtaining more accurate prediction results for brain tumor segmentation. The current mainstream research approaches obtain segmentation accuracy improvements by modifying deep-learning model architectures, while ignoring the computational and storage efficiency issues of segmentation. In this paper, we proposed an improved knowledge distillation method: coordinate distillation (CD), which integrates channel and space information and completes brain tumor segmentation by training the student network with the teacher network without changing the original network architecture. Experimental results showed that the method was effective and that it could enhance the segmentation accuracy of brain tumors without changing the segmentation efficiency.
Effects of Kerr Nonlinearity in Physical Unclonable Functions
Authors: Nikolopoulos Georgios M.
Published: November 2022

We address the question of whether the presence of Kerr nonlinearity in multiple-scattering optical media offers any advantage with respect to the design of physical unclonable functions. Our results suggest that under certain conditions, nonlinear physical unclonable functions can be more robust against the potential cloning of the medium relative to their linear counterparts that have been exploited in the context of various cryptographic applications.
Experimental Determination of the Coefficient of Friction on a Screw Joint
Authors: Mascenik Jozef , Coranic Tomas
Published: November 2022

This paper deals with the coefficient of determination of screw connection friction between the thread and the matrix. The coefficient of friction was measured using a laboratory device with an M20 screw connection without any grease and, subsequently, plastic grease was added (CX80 silicone, lithium, and copper grease). When grease is added, the friction in the threads and screw heads is limited and consistently retained. When tightening by torque, which represents the prevailing assembly method in standard screwing practice, only part of the torque is effectively used to create axial force and pre-stress. The rest of the torque is employed in friction suppression between threads and converted into heat. In general, the coefficient of friction depends on diverse factors such as the roughness of the thread surface, the gradient angle of the helix, and the grease properties. The tightening torque represents a primary parameter in the experimental measurements, monitored using a digital torque spanner, and generates an axial force in the screw. Based on the aforementioned parameters, the objective of this paper was to monitor changes in the coefficient of friction between the thread of the screw and the matrix in the case of different grease types. The actual coefficient of friction was calculated through the exponential equation of the torque balance. First measured was the load of the bolted joint without the use of grease, where the average value of the coefficient of friction was 0.44732; this value served as a benchmark for comparison to the measurements with the use of grease. The measurements showed that the value of the friction coefficient was reduced by 30.57% when using lithium grease, by 40.56% when using silicone grease, and by 47.64% when using copper-based grease, making the latter the most suitable for the application. Without appropriate greasing, friction suppression was accompanied by extremely high torques, which resulted in insufficient screw prolongation.
Is It Safe to Use Vasoconstrictors in Association Treated with Amitriptyline or Can It Potentiate Cardiovascular Effects? In Vivo Animal Study
Authors: Oliveira Gabriela Moraes , Dionísio Thiago José , Fleury Camila Assis , Calvo Adriana Maria , Santos Carlos Ferreira , Faria Flavio Augusto Cardoso
Published: November 2022

This study aimed to evaluate changes in blood pressure of rats treated or not with amitriptyline after infiltration in the buccal sulcus and intravenous injection of epinephrine, felypressin and phenylephrine in equivalent doses (ED) to the amounts present in 2, 8 and 32 local anesthetic tubes. 42 male Wistar rats, with 45-day-old, treated for seven days with amitriptyline hydrochloride (0.3 mg/kg). On the eighth day, the animal was submitted to general anesthesia and surgery for direct blood pressure rate. The significance level was 5%. The treatment with amitriptyline caused a significant decrease in blood pressure of the treated group compared to the control group (101.80 ± 2.52 and 110.12 ± 2.91 mmHg, respectively, * p < 0.05), and slightly potentiates the hypertensive response after infiltration of epinephrine (4.11 ± 0.54; 7.15 ± 0.55; 9.03 ± 0.87 mmHg, respectively, 2, 8 and 32 tubes, p > 0.05). Felypressin promotes lower blood pressure changes and phenylephrine proved to be the most potent vasoconstrictor of the three studied, producing important changes in blood pressure and, even though infiltration, in doses greater than 8 tubes (15.43 ± 1.15; 70.62 ± 3.70 mmHg, respectively, 8 and 32 tubes, * p < 0.05). The infiltration of the three vasoconstrictors in doses equal to or less than 8 tubes does not cause significant changes in blood pressure, both in the control and amitriptyline treated groups.
Spatiotemporal Patterns of Air Pollution in an Industrialised City—A Case Study of Ust-Kamenogorsk, Kazakhstan
Authors: Assanov Daulet , Radelyuk Ivan , Perederiy Olessya , Galkin Stanislav , Maratova Gulira , Zapasnyi Valeriy , Klemeš Jiří Jaromír
Published: November 2022

Air quality issues still affect the quality of life for people in industrialised cities around the world. The investigations should include the identification of the sources of the pollution and its distribution in space and time. This work is the first attempt to perform identification of the sources of pollution in Ust-Kamenogorsk city in Kazakhstan. Analysis of retrospective data (including ten variables (TSP, SO2, CO, NO2, phenol, HF, HCl, H2SO4, formaldehyde, H2S) from five monitoring stations for the period 2017–2021) using multivariate statistical methods and hierarchical cluster analysis has been performed to assess spatiotemporal patterns of air quality of the city. The results indicate that the contamination patterns can be grouped into two categories: cold and warm seasons. The study revealed the dangerous concentrations of NO2 and SO2 exceeded the limits by 2–3 and 1.5–2 times, independently of the seasonality. Averaged concentrations of TSP slightly exceeded the established limits for the most industrialised part of the city. Concentrations of HF and formaldehyde significantly rose during the cold seasons compared to the warm seasons. Other chemical parameters significantly depend on the seasonality and locations of the sampling points. The major reason for air pollution is twofold—the use of a burnt-coal throughout the year for electricity and heat generation (especially during the cold seasons) and the high density of the heavy metallurgy industry in the city. The principal component analysis confirms a high loading of industrial sources of air pollution on both spatial and seasonal dimensions.
Sol–Gel Synthesis of LiTiO2 and LiBO2 and Their CO2 Capture Properties
Authors: Li Liang , Yu Haidi , Chen Yuqi
Published: November 2022

LiTiO2 was prepared from tetraethoxy titanium and lithium ethoxide by a sol–gel process and then treated at 773 K and 973 K under oxygen atmosphere, respectively. Compared with LiTiO2 prepared at 973 K, LiTiO2 prepared at 773 K has better CO2 capture properties. XRD patterns of synthetic LiTiO2 before and after CO2 capture confirm that the intermediate product, LixTizO2, is produced during CO2 capture. CO2 absorption degree of LiTiO2 was determined to be 37% (293 K), 40.8% (333 K), 45.5% (373 K), and 50.1% (393 K) for 11.75 h, respectively. Repetitive CO2 capture experiment indicates that LiTiO2 has excellent cyclic regeneration behavior. The CO2 absorption degree of LiTiO2 increased with increasing CO2 concentration. At a concentration of 0.05%, the absorption degree of LiTiO2 had a stable value of 1% even after an absorption time of 1.4 h. LiBO2 was fabricated by the similar sol–gel method and treated at 713 K. Mass percentage and specific surface area of synthesized LiBO2 increased with the increasing absorption temperature. Evidently, the diffusion of the CO2 molecule through the reaction product, which had a low activation energy of 15 kJ·mol−1 and apparent specific surface value of 55.63 m2/g, determined the efficiency of the absorption reaction. Compared with the other sol–gel synthesized lithium-based oxides, LiTiO2 possessed higher absorption capabilities and lower desorption temperature.
Changes in Preexisting Temporomandibular Joint Clicking after Orthognathic Surgery in Patients with Mandibular Prognathism
Authors: Chen Chun-Ming , Chen Pei-Jung , Hsu Han-Jen
Published: November 2022

This study aimed to investigate the changes in preexisting temporomandibular joint (TMJ) clicking and the new incidence of TMJ clicking after orthognathic surgery. A total of 60 patients (30 men and 30 women) with mandibular prognathism underwent intraoral vertical ramus osteotomy (IVRO) for a mandibular setback. The setback amount and TMJ clicking symptoms (preoperative and one year postoperative) were recorded. To assess the risk of new incidence of TMJ clicking in asymptomatic patients, the cutoff value for postoperative mandibular setback was set at 8 mm. The left and right mandibular setbacks were 11.1 and 10.9 mm in men, respectively, and 10.7 and 10.0 mm in women, respectively. Thus, no difference in setback amount on either side was observed between the sexes. The improvement rate in patients with preexisting TMJ clicking was 69.2% (18 of 26 sides); the postoperative improvement rates were 71.4% (setback amount > 8 mm) and 60% (setback amount ≤ 8 mm). IVRO may reduce the severity of preexisting TMJ clicking. A high setback amount (>8 mm) may not be associated with a considerable increase in the risk of postoperative TMJ clicking.
Evaluation of B7-H3 Targeted Immunotherapy in a 3D Organoid Model of Craniopharyngioma
Authors: Tang Mei , Chen Caili , Wang Guoqing , Wang Yuelong , Zhang Zongliang , Li Hexian , Lu Qizhong , Wang Zeng , Zhao Shasha , Yang Chen , Zhong Kunhong , Zhang Ruyuan , Guo Liping , Yuan Zhu , Nie Chunlai , Tong Aiping
Published: November 2022

A craniopharyngioma (CP) is a rare epithelial tumor of the sellar and parasellar region. CPs are difficult to treat due to their anatomical proximity to critical nervous structures, which limits the ability of the surgeon to completely resect the lesion, exposing patients to a high risk of recurrence. The treatment of craniopharyngiomas is primarily surgery and radiotherapy. So far, neither a cell line nor an animal model has been established, and thus data on other treatment options, such as chemotherapy and immunotherapy, are limited. Here, the expression profile of the pan-cancer antigen B7-H3 in various cancer types including CP was examined by immunohistochemistry. An in vitro organoid model was established by using fresh tissue biospecimens of CP. Based on the organoid model, we evaluated the antitumor efficacy of B7-H3-targeted immunotherapy on CP. As a result, the highest expression of B7-H3 was observed in CP tissues across various cancer types. Although B7-H3-targeted chimeric antigen-receptor T cells show obvious tumor-killing effects in the traditional 2D cell culture model, limited antitumor effects were observed in the 3D organoid model. The B7-H3-targeted antibody-DM1 conjugate exhibited a potent tumor suppression function both in 2D and 3D models. In conclusion, for the first time, we established an organoid model for CP and our results support that B7-H3 might serve as a promising target for antibody-drug conjugate therapy against craniopharyngioma.
Influence of the Immune Microenvironment Provided by Implanted Biomaterials on the Biological Properties of Masquelet-Induced Membranes in Rats: Metakaolin as an Alternative Spacer
Authors: Durand Marjorie , Oger Myriam , Nikovics Krisztina , Venant Julien , Guillope Anne-Cecile , Jouve Eugénie , Barbier Laure , Bégot Laurent , Poirier Florence , Rousseau Catherine , Pitois Olivier , Mathieu Laurent , Favier Anne-Laure , Lutomski Didier , Collombet Jean-Marc
Published: November 2022

Macrophages play a key role in the inflammatory phase of wound repair and foreign body reactions—two important processes in the Masquelet-induced membrane technique for extremity reconstruction. The macrophage response depends largely on the nature of the biomaterials implanted. However, little is known about the influence of the macrophage microenvironment on the osteogenic properties of the induced membrane or subsequent bone regeneration. We used metakaolin, an immunogenic material, as an alternative spacer to standard polymethylmethacrylate (PMMA) in a Masquelet model in rats. Four weeks after implantation, the PMMA- and metakaolin-induced membranes were harvested, and their osteogenic properties and macrophage microenvironments were investigated by histology, immunohistochemistry, mass spectroscopy and gene expression analysis. The metakaolin spacer induced membranes with higher levels of two potent pro-osteogenic factors, transforming growth factor-β (TGF-β) and bone morphogenic protein-2 (BMP-2). These alternative membranes thus had greater osteogenic activity, which was accompanied by a significant expansion of the total macrophage population, including both the M1-like and M2-like subtypes. Microcomputed tomographic analysis showed that metakaolin-induced membranes supported bone regeneration more effectively than PMMA-induced membranes through better callus properties (+58%), although this difference was not significant. This study provides the first evidence of the influence of the immune microenvironment on the osteogenic properties of the induced membranes.
The Long-Term Effects of Prenatal Hypoxia on Coronary Artery Function of the Male and Female Offspring
Authors: Hula Nataliia , Liu Ricky , Spaans Floor , Pasha Mazhar , Quon Anita , Kirschenman Raven , Cooke Christy-Lynn M. , Davidge Sandra T.
Published: November 2022

Prenatal hypoxia predisposes the offspring to the development of cardiovascular (CV) dysfunction in adult life. Using a rat model, we assessed the effect of prenatal hypoxia on vasoconstrictive and vasodilative mechanisms in left anterior descending coronary arteries of 4- and 9.5-month-old offspring. Endothelium-dependent relaxation to methylcholine and vasoconstriction responses to endothelin-1 (ET-1) were assessed by wire myography. Prenatal hypoxia impaired endothelium-dependent vasodilation in 4- and 9.5-month-old offspring. Inhibition of nitric oxide (NO) synthase prevented coronary artery relaxation in all groups. Inhibition of prostaglandin H synthase (PGHS) improved relaxation in prenatally hypoxic males and tended to improve vasorelaxation in females, suggesting that impaired vasodilation was mediated via increased PGHS-dependent vasoconstriction. An enhanced contribution of endothelium-dependent hyperpolarization to coronary artery vasodilation was observed in prenatally hypoxic males and females. No changes in endothelial NO synthase (eNOS) and PGHS-1 expressions were observed, while PGHS-2 expression was decreased in only prenatally hypoxic males. At 4 months, ET-1 responses were similar between groups, while ETB inhibition (with BQ788) tended to decrease ET-1-mediated responses in only prenatally hypoxic females. At 9.5 months, ET-1-mediated responses were decreased in only prenatally hypoxic females. Our data suggest that prenatal hypoxia has long-term similar effects on the mechanisms of impaired endothelium-dependent vasodilation in coronary arteries from adult male and female offspring; however, coronary artery contractile capacity is impaired only in prenatally hypoxic females. Understanding the mechanistic pathways involved in the programming of CV disease may allow for the development of therapeutic interventions.
Mitochondrial Ca2+ Signaling and Bioenergetics in Alzheimer’s Disease
Authors: Arnst Nikita , Redolfi Nelly , Lia Annamaria , Bedetta Martina , Greotti Elisa , Pizzo Paola
Published: November 2022

Alzheimer’s disease (AD) is a hereditary and sporadic neurodegenerative illness defined by the gradual and cumulative loss of neurons in specific brain areas. The processes that cause AD are still under investigation and there are no available therapies to halt it. Current progress puts at the forefront the “calcium (Ca2+) hypothesis” as a key AD pathogenic pathway, impacting neuronal, astrocyte and microglial function. In this review, we focused on mitochondrial Ca2+ alterations in AD, their causes and bioenergetic consequences in neuronal and glial cells, summarizing the possible mechanisms linking detrimental mitochondrial Ca2+ signals to neuronal death in different experimental AD models.
Mucosal Immune System Dysregulation in the Pathogenesis of IgA Nephropathy
Authors: Kano Toshiki , Suzuki Hitoshi , Makita Yuko , Nihei Yoshihito , Fukao Yusuke , Nakayama Maiko , Lee Mingfeng , Kato Rina , Aoki Ryosuke , Yamada Koshi , Muto Masahiro , Suzuki Yusuke
Published: November 2022

The mucosal immune system, via a dynamic immune network, serves as the first line of defense against exogenous antigens. Mucosal immune system dysregulation is closely associated with the pathogenesis of immunoglobulin A nephropathy (IgAN), as illustrated by IgAN having the clinical feature of gross hematuria, often concurrent with mucosal infections. Notably, previous studies have demonstrated the efficacy of tonsillectomy and found that a targeted-release formulation of budesonide reduced proteinuria in patients with IgAN. However, it remains unclear how exogenous antigens interact with the mucosal immune system to induce or exacerbate IgAN. Thus, in this review, we focus on the dysregulation of mucosal immune response in the pathogenesis of IgAN.
Characterization of Decellularized Extracellular Matrix from Milkfish (Chanos chanos) Skin
Authors: Bual Ronald , Labares Marionilo , Valle Kit Dominick Don , Pague Job , Bantilan Zesreal Cain , Ducao Princess Grace , Alimasag Johnel , Acibar Catherine
Published: November 2022

Milkfish (Chanos chanos) is an abundant fish commodity in the Philippines that generates a large number of wastes such as skin, scales, viscera, and bones, which, upon disposal, cause environmental pollution. The abundance of these wastes, such as fish skin, rich in bioactive natural products such as collagen, elicits interest in their conversion into high-market-value products. The decellularization of milkfish skin waste can extract its extracellular matrix (ECM), a potential raw material for biomedical applications such as the repair of damaged skin tissues. In particular, this study characterized the developed decellularized ECM with different concentrations (0.1%, 1.0%) of the decellularizing agents (Triton X-100, SDS) and temperature (4 °C, room temperature) using milkfish skin. The decellularized ECM structure was better preserved using Triton X-100, while SDS was more effective in cell component removal, especially at 1% concentration and 4 °C temperature. There were significant effects of varying the temperatures and concentrations on the physical and mechanical properties of the decellularized ECM. Future studies could explore more variables to further establish protocols and more analyses to better characterize the decellularized milkfish skin.
Consumer Behaviour to Be Considered in Advertising: A Systematic Analysis and Future Agenda
Authors: Alsharif Ahmed H. , Salleh Nor Zafir Md , Al-Zahrani Shaymah Ahmed , Khraiwish Ahmad
Published: November 2022

In the past decade, neurophysiological and physiological tools have been used to explore consumer behaviour toward advertising. The studies into brain processes (e.g., emotions, motivation, reward, attention, perception, and memory) toward advertising are scant, and remain unclear in the academic literature. To fill the gap in the literature, this study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol to extract relevant articles. It extracted and analysed 76 empirical articles from the Web of Science (WoS) database from 2009–2020. The findings revealed that the inferior frontal gyrus was associated with pleasure, while the middle temporal gyrus correlated with displeasure of advertising. Meanwhile, the right superior-temporal is related to high arousal and the right middle-frontal-gyrus is linked to low arousal toward advertisement campaigns. The right prefrontal-cortex (PFC) is correlated with withdrawal behaviour, and the left PFC is linked to approach behaviour. For the reward system, the ventral striatum has a main role in the reward system. It has also been found that perception is connected to the orbitofrontal cortex (OFC) and ventromedial (Vm) PFC. The study’s findings provide a profound overview of the importance of brain processes such as emotional processes, reward, motivation, cognitive processes, and perception in advertising campaigns such as commercial, social initiative, and public health.
Antecedents of Rural Tourism Experience Memory: Tourists’ Perceptions of Tourism Supply and Positive Emotions
Authors: Chen Hang , Wang Yuewei , Zou Minglu , Li Jiaxin
Published: November 2022

Tourism experiences bring about physical or psychological feelings in tourists, which can not only leave tourists with deep memories, but also affect their behavioral intentions. Tourism experiences are meaningful only if they can be remembered and influence word of mouth and decision making. A better understanding of what influences tourism experience memory will help optimize the supply and further development of tourism destinations. This study explores the antecedents of rural tourism experience memory from the tourism supply perspective, revealing the mechanism of effect of these antecedents on tourists’ tourism experience memory formation through a questionnaire-based survey of 556 participants in Xidi Village, China, and correlation and multiple regression analyses. The results show that perceptions about the supply of rural tourism destinations trigger positive emotions that, in turn, affect the formation of rural tourism experience memory. Through the mediating role of positive emotions, there is a significant correlation between perception of rural tourism destination supply and the formation of rural tourism experience memories.
HER2-Positive Gastroesophageal Cancers Are Associated with a Higher Risk of Brain Metastasis
Authors: Tincknell Gary , Naveed Asma , Nankervis Jane , Mukhtiar Ayesha , Piper Ann-Katrin , Becker Therese M. , Chantrill Lorraine , Aghmesheh Morteza , Vine Kara Lea , Ranson Marie , Brungs Daniel
Published: November 2022

Brain metastasis from gastroesophageal adenocarcinomas (GOCs) is a rare but a devastating diagnosis. Human epidermal growth factor receptor 2 (HER2) is a prognostic and predictive biomarker in GOCs. The association of HER2 with GOC brain metastasis is not known. We performed a retrospective analysis of patients with GOCs with known HER2 status between January 2015 and November 2021. HER2 was assessed on either the primary tumour or metastasis by immunohistochemistry or in situ hybridization. The diagnosis of brain metastasis was made on standard imaging techniques in patients with symptoms or signs. HER2 results were available for 201 patients, with 34 patients (16.9%) HER2 positive. A total of 12 patients developed symptomatic brain metastasis from GOCs, of which 7 (58.3%) were HER2 positive. The development of symptomatic brain metastasis was significantly higher in the HER2-positive GOCs (OR8.26, 95%CI 2.09–35.60; p = 0.0009). There was no significant association of HER2 status and overall survival in patients with brain metastasis. Although the rate of brain metastasis remains low in GOCs, the incidence of symptomatic brain metastasis was significantly higher in patients with HER2-positive tumours.
Progress on Denosumab Use in Giant Cell Tumor of Bone: Dose and Duration of Therapy
Authors: Xiang Feifan , Liu Huipan , Deng Jia , Ma Wenzhe , Chen Yue
Published: November 2022

Giant cell tumor of bone (GCTB) is an aggressive non-cancerous bone tumor associated with risks of sarcoma and metastasis. Once malignancy occurs, the prognosis is generally poor. Surgery remains the main treatment for GCTB. Multidisciplinary management is a feasible option for patients wherein surgical resection is not an option or for those with serious surgery-related complications. Denosumab is an anti-nuclear factor kappa B ligand approved for the treatment of postmenopausal women with osteoporosis, bone metastases, and advanced or inoperable GCTB. However, the guidelines for treating GCTB are unclear; its short-term efficacy and safety in inoperable patients have been demonstrated. Lengthier therapies (high cumulative doses) or pre-operative adjuvant therapy may be associated with severe complications and high local recurrence rates. Short-term administration helps attain satisfactory local control and functionality. As a result, lately, the impact of different doses and lengths of treatment on the efficacy of denosumab in GCTB treatment, the incidence of complications, and recurrence rates have gained attention. The efficacy and safety of denosumab against GCTB, its impact on imaging assessment, related complications, and recurrence of GCTB were previously reviewed. For further research direction, this paper reviews the progress of studies evaluating the impact of the dose and duration of denosumab therapy for GCTB.
Choice of High-Throughput Proteomics Method Affects Data Integration with Transcriptomics and the Potential Use in Biomarker Discovery
Authors: Mosquim Junior Sergio , Siino Valentina , Rydén Lisa , Vallon-Christersson Johan , Levander Fredrik
Published: November 2022

In recent years, several advances have been achieved in breast cancer (BC) classification and treatment. However, overdiagnosis, overtreatment, and recurrent disease are still significant causes of complication and death. Here, we present the development of a protocol aimed at parallel transcriptome and proteome analysis of BC tissue samples using mass spectrometry, via Data Dependent and Independent Acquisitions (DDA and DIA). Protein digestion was semi-automated and performed on flowthroughs after RNA extraction. Data for 116 samples were acquired in DDA and DIA modes and processed using MaxQuant, EncyclopeDIA, or DIA-NN. DIA-NN showed an increased number of identified proteins, reproducibility, and correlation with matching RNA-seq data, therefore representing the best alternative for this setup. Gene Set Enrichment Analysis pointed towards complementary information being found between transcriptomic and proteomic data. A decision tree model, designed to predict the intrinsic subtypes based on differentially abundant proteins across different conditions, selected protein groups that recapitulate important clinical features, such as estrogen receptor status, HER2 status, proliferation, and aggressiveness. Taken together, our results indicate that the proposed protocol performed well for the application. Additionally, the relevance of the selected proteins points to the possibility of using such data as a biomarker discovery tool for personalized medicine.