bluuebunny/crossref_metadata_2025 huggingface dataset:
Flow and Convection in Metal Foams: A Survey and New CFD Results
Authors: Pulvirenti Beatrice , Celli Michele , Barletta Antonio
Published: September 2020

Metal foams are widely studied as possible tools for the enhancement of heat transfer from hot bodies. The basic idea is that a metal foam tends to significantly increase the heat exchange area between the hot solid body and the external cooling fluid. For this reason, this class of porous materials is considered as a good candidate for an alternative to finned surfaces, with different pros and cons. Among the pros, we mention the generally wider area of contact per unit volume between solid and fluid. Among the cons is the difficulty to produce different specimens with the same inner structure, with the consequence that their performance may be significantly variable. This paper will offer a survey of the literature with a focus on the main heat transfer characteristics of the metal foams and the energy balance model based on Local Thermal Non-Equilibrium (LTNE). Then, a numerical simulation of the heat transfer at the pore-scale level for an artificial foam with a spatially periodic structure will be discussed. Finally, these numerical results will be employed to assess the macroscopic modeling of the flow and heat transfer in a metal foam. More precisely, the Darcy–Forchheimer model and the LTNE model adopted to describe the momentum and energy transfer in metal foams have been validated for metallic periodic structures.
Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces
Authors: Azinheiro Sarah , Carvalho Joana , Prado Marta , Garrido-Maestu Alejandro
Published: September 2020

The continuous contamination of foods with L. monocytogenes, highlights the need for additional controls in the food industry. The verification of food processing plants is key to avoid cross-contaminations, and to assure the safety of the food products. In this study, a new methodology for the detection of L. monocytogenes on food contact surfaces was developed and evaluated. It combines Recombinase Polymerase Amplification (RPA) with the lateral flow (LF) naked-eye detection. Different approaches for the recovery of the bacteria from the surface, the enrichment step and downstream analysis by RPA-LF were tested and optimized. The results were compared with a standard culture-based technique and qPCR analysis. Sampling procedure with sponges was more efficient for the recovery of the bacteria than a regular swab. A 24 h enrichment in ONE broth was needed for the most sensitive detection of the pathogen. By RPA-LF, it was possible to detect 1.1 pg/µL of pure L. monocytogenes DNA, and the complete methodology reached a LoD50 of 4.2 CFU/cm2 and LoD95 of 18.2 CFU/cm2. These results are comparable with the culture-based methodology and qPCR. The developed approach allows for a next-day detection without complex equipment and a naked-eye visualization of the results.
Addition of Anionic Polysaccharide Stabilizers Modulates In Vitro Digestive Proteolysis of a Chocolate Milk Drink in Adults and Children
Authors: David Shlomit , Magram Klaiman Maya , Shpigelman Avi , Lesmes Uri
Published: September 2020

There is a need to better understand the possible anti-nutritional effect of food stabilizers on the digestibility of important macronutrients, like proteins. This study hypothesized that the anionic nature of κ-, ι-, λ-, Carrageenan (CGN) and xanthan gum directs their interactions with food proteins leading to their subsequent attenuated digestive proteolysis. Model chocolate milk drinks were tested for their colloidal properties, viscosity and proteolytic breakdown in adults and children using in vitro digestion models coupled with proteomic analyses. SDS-PAGE analyses of gastro-intestinal effluents highlight stabilizers hinder protein breakdown in adults and children. Zeta potential and colloidal particle size were the strongest determinants of stabilizers’ ability to hinder proteolysis. LC-MS proteomic analyses revealed stabilizer addition significantly reduced bioaccessibility of milk-derived bioactive peptides with differences in liberated peptide sequences arising mainly from their location on the outer rim of the protein structures. Further, liberation of bioactive peptides emptying from a child stomach into the intestine were most affected by the presence of ι-CGN. Overall, this study raises the notion that stabilizer charge and other properties of edible proteins are detrimental to the ability of humans to utilize the nutritional potential of such formulations. This could help food professionals and regulatory agencies carefully consider the use of anionic stabilizers in products aiming to serve as protein sources for children and other liable populations.
Characteristics of Atmospheric Circulation Associated with Variability of Sea Ice in the Arctic
Authors: Platov Gennady , Iakshina Dina , Krupchatnikov Vladimir
Published: September 2020

The paper investigates the role of atmospheric circulation in the surface layer in forming the Arctic ice structure. For the analysis, the empirical orthogonal function (EOF) method of decomposition of the surface wind field is used, and the reaction of ice to changes in the principal components of leading EOF modes is investigated using statistical methods. Analyzing the rate of ice change in the Arctic associated with the Arctic ocean oscillation mode, we concluded that this mode’s variability leads to the formation of a seesaw in the ice field between two regions. From the one side, it is the region of the central deep-water part of the Arctic, including the East Siberian Sea, and from the other side, it is all other marginal seas. The second (“dipole”) mode is most associated with an increase/decrease in the ice thickness at the Arctic exit through the Fram Strait, as well as the formation of the so-called “ice factory” in the coastal region of the Beaufort Sea in the positive phase of this mode. There is also a significant relationship between the variability of third mode and the arrival of Atlantic waters with a high heat content into the Arctic through the Barents opening, which creates preconditions for ice formation in this region.
Formula for the Cross-Sectional Area of the Muscles of the Third Lumbar Vertebra Level from the Twelfth Thoracic Vertebra Level Slice on Computed Tomography
Authors: Ishida Yuria , Maeda Keisuke , Yamanaka Yosuke , Matsuyama Remi , Kato Ryoko , Yamaguchi Makoto , Nonogaki Tomoyuki , Shimizu Akio , Ueshima Junko , Murotani Kenta , Mori Naoharu
Published: September 2020

The purpose of this study was to investigate a means by which to reflect muscle mass using chest computed tomography (CT). A cross-sectional study was conducted with patients aged ≥ 65 years having abdominal and chest CT scans. The formula to predict third lumbar vertebra (L3) cross-sectional area (CSA) of the muscles from the erector muscles of the spine at the twelfth thoracic vertebra (Th12) level slice on CT was created using the five-fold cross-validation method. Correlation between predicted L3 CSA and measured L3 CSA of the muscles was assessed by intraclass correlation coefficients (ICC) and correlation coefficients (r) in the data of the development, and predictability was examined with accuracy and F-values in the validation study. The development study included 161 patients. The developed formula was as follows: −1006.38 + 16.29 × age [years] + 1161.80 × sex (if female, 0; if male, 1) + 55.91 × body weight [kg] + 2.22 × CSA of the erector muscles at Th12 [mm2]. The formula demonstrated strong concordance and correlation (ICC = 0.849 [0.800–0.887] and r = 0.858 [0.811–0.894]). The validation study included 34 patients. The accuracy and F-value between predicted CSA and measured CSA were high (accuracy = 0.889–0.944, F-value = 0.931–0.968). We developed a formula predicting CSA at L3 using Th12 CT slice. This formula could be used to assess decreased muscle mass even with chest CT alone.
Experimental Hypomagnesemia Induces Neurogenic Inflammation and Cardiac Dysfunction
Authors: Kramer Jay H. , Mak I. Tong , Chmielinska Joanna J. , Spurney Christopher F. , Phillips Terry M. , Weglicki William B.
Published: September 2020

Hypomagnesemia occurs clinically as a result of restricted dietary intake, Mg-wasting drug therapies, chronic disease status and may be a risk factor in patients with cardiovascular disorders. Dietary restriction of magnesium (Mg deficiency) in animal models produced a pro-inflammatory/pro-oxidant condition, involving hematopoietic, neuronal, cardiovascular, renal and other systems. In Mg-deficient rodents, early elevations in circulating levels of the neuropeptide, substance P (SP) may trigger subsequent deleterious inflammatory/oxidative/nitrosative stress events. Evidence also suggests that activity of neutral endopeptidase (NEP, neprilysin), the major SP-degrading enzyme, may be impaired during later stages of Mg deficiency, and this may sustain the neurogenic inflammatory response. In this article, experimental findings using substance P receptor blockade, NEP inhibition, and N-methyl-D-aspartate (NMDA) receptor blockade demonstrated the connection between hypomagnesemia, neurogenic inflammation, oxidative stress and enhanced cardiac dysfunction. Proof of concept concerning neurogenic inflammation is provided using an isolated perfused rat heart model exposed to acute reductions in perfusate magnesium concentrations.
Recommendations for Effective Intersectoral Collaboration in Health Promotion Interventions: Results from Joint Action CHRODIS-PLUS Work Package 5 Activities
Authors: van Dale Djoeke , Lemmens Lidwien , Hendriksen Marieke , Savolainen Nella , Nagy Péter , Marosi Edit , Eigenmann Michela , Stegemann Ingrid , Rogers Heather L.
Published: September 2020

The burden of chronic disease in Europe continues to grow. A major challenge facing national governments is how to tackle the risk factors of sedentary lifestyle, alcohol abuse, smoking, and unhealthy diet. These factors are complex and necessitate intersectoral collaboration to strengthen health promotion, counter-act the social determinants of health, and reduce the prevalence of chronic disease. European countries have diverse intersectoral collaboration to encourage health promotion activities. In the Joint Action CHRODIS-PLUS success factors for intersectoral collaboration within and outside healthcare which strengthen health promotion activities were identified with a mixed method design via a survey of 22 project partners in 14 countries and 2 workshops. In six semi-structured interviews, the mechanisms underlying these success factors were examined. These mechanisms can be very context-specific but do give more insight into how they can be replicated. In this paper, 20 health promotion interventions from national programs in CHRODIS PLUS are explored. This includes community interventions, policy actions, integrated approaches, capacity building, and training activities. The interventions involved collaboration across three to more than six sectors. The conclusion is a set of seven recommendations that are considered to be essential for fostering intersectoral collaboration to improve health-promoting activities.
Sudden Sensorineural Hearing Loss Associated with Nutritional Anemia: A Nested Case–Control Study Using a National Health Screening Cohort
Authors: Kim So Young , Wee Jee Hye , Min Chanyang , Yoo Dae-Myoung , Choi Hyo Geun
Published: September 2020

Previous studies have suggested an association of anemia with hearing loss. The aim of this study was to investigate the association of nutritional anemia with sudden sensorineural hearing loss (SSNHL), as previous studies in this aspect are lacking. We analyzed data from the Korean National Health Insurance Service-Health Screening Cohort 2002–2015. Patients with SSNHL (n = 9393) were paired with 37,572 age-, sex-, income-, and region of residence-matched controls. Both groups were assessed for a history of nutritional anemia. Conditional logistic regression analyses were performed to calculate the odds ratios (ORs) (95% confidence interval, CI) for a previous diagnosis of nutritional anemia and for the hemoglobin level in patients with SSNHL. Subgroup analyses were conducted for age and sex. Age, sex, income, and region of residence were stratified. Obesity, smoking, drinking alcohol, systolic/diastolic blood pressure, fasting blood glucose, total cholesterol, and the Charlson Comorbidity Index were considered in the regression models. Nutritional anemia was present in 4.8% (449/9393) of patients with SSNHL and 4.0% (1494/37,572) of controls (p < 0.001). The SSNHL group demonstrated 1.20-fold higher odds for nutritional anemia (95% CI = 1.08–1.34, p = 0.001). Hemoglobin levels were not associated with SSNHL. In subgroups <60 years old, there was a consistent positive association of nutritional anemia with SSNHL (adjusted OR = 1.55, 95% CI = 1.11–2.15, p = 0.010 for men <60 years old, and adjusted OR = 1.22, 95% CI = 1.02–1.45, p = 0.028 for women <60 years old). Nutritional anemia, but not hemoglobin level, was associated with an increased risk of SSNHL.
The Post-Coronavirus World in the International Tourism Industry: Application of the Theory of Planned Behavior to Safer Destination Choices in the Case of US Outbound Tourism
Authors: Han Heesup , Al-Ansi Amr , Chua Bee-Lia , Tariq Beenish , Radic Aleksandar , Park Su-hyun
Published: September 2020

The tourism industry has been seriously suffering from the coronavirus disease (COVID-19) crisis ever since its outbreak. Given this pandemic situation, the major aim of this study is to develop a conceptual framework that clearly explains the US international tourists’ post-pandemic travel behaviors by expanding the theory of planned behavior (TPB). By utilizing a quantitative process, the TPB was successfully broadened by incorporating the travelers’ perceived knowledge of COVID-19, and it has been deepened by integrating the psychological risk. Our theoretical framework sufficiently accounted for the US tourists’ post-pandemic travel intentions for safer international destinations. In addition, the perceived knowledge of COVID-19 contributed to boosting the prediction power for the intentions. The associations among the subjective norm, the attitude, and the intentions are under the significant influence of the tourists’ psychological risks regarding international traveling. The comparative criticality of the subjective norm is found. Overall, the findings of this study considerably enhanced our understanding of US overseas tourists’ post-pandemic travel decision-making processes and behaviors.
Convenience Store Use and the Health of Urban Adolescents in Seoul, South Korea
Authors: Yoon Nan-He , Shon Changwoo
Published: September 2020

To improve urban adolescents’ dietary behaviors and health, factors that influence them to buy meals in convenience stores with regard to urban food environments must be determined. This study investigated the factors which influence adolescents’ substitution of meals with convenience store meals and its impact on their health in Seoul (South Korea). Multilevel analysis and logistic regression analysis were conducted using data from the Korea Youth Risk Behavior Survey, a national health survey with a representative sample of Korean adolescents. Among 17,624 teenagers who responded to surveys in 2017 and 2019, 30.5% of them substituted meals with convenience store meals more than three times a week. Girls and students with a lower family economic level were more likely to frequently consume food from convenience stores. Unhealthy lifestyles and poor mental health status also influenced their decisions to substitute meals with convenience store food. Those who frequently consumed meals from convenience stores were more likely to have unhealthy lifestyles, featuring bad diets, smoking, drinking, and sedentary behaviors. They also reported significantly poorer self-rated health and mental health. To promote healthy lifestyles among adolescents, efforts to raise awareness and develop supportive environments for healthy diets are strongly recommended.
Reply to Comment on “Environmental and Occupational Risk Factors of Amyotrophic Lateral Sclerosis: A Population-Based Case-Control Study”
Authors: Filippini Tommaso , Tesauro Marina , Fiore Maria , Malagoli Carlotta , Consonni Michela , Violi Federica , Iacuzio Laura , Arcolin Elisa , Oliveri Conti Gea , Cristaldi Antonio , Zuccarello Pietro , Zucchi Elisabetta , Mazzini Letizia , Pisano Fabrizio , Gagliardi Ileana , Patti Francesco , Mandrioli Jessica , Ferrante Margherita , Vinceti Marco
Published: September 2020

We much appreciate the positive comments and interest concerning our study on the environmental and occupational risk factors of amyotrophic lateral sclerosis (ALS) [...]
A Scoping Review on Cue Reactivity in Methamphetamine Use Disorder
Authors: Seow Lee Seng Esmond , Ong Wei Jie , Hombali Aditi , AshaRani P. V. , Subramaniam Mythily
Published: September 2020

The experience of craving via exposure to drug-related cues often leads to relapse in drug users. This study consolidated existing empirical evidences of cue reactivity to methamphetamine to provide an overview of current literature and to inform the directions for future research. The best practice methodological framework for conducting scoping review by Arkey and O’Malley was adopted. Studies that have used a cue paradigm or reported on cue reactivity in persons with a history of methamphetamine use were included. Databases such as Medline, EMBASE, PsycINFO and CINAHL were searched using key terms, in addition to citation check and hand search. The search resulted in a total of 32 original research articles published between 2006 to 2020. Three main themes with regard to cue reactivity were identified and synthesized: (1) effects of cue exposure, (2) individual factors associated with cue reactivity, and (3) strategies that modulate craving or reactivity to cues. Exposure to methamphetamine-associated cues elicits significant craving and other autonomic reactivity. Evidence suggests that drug cue reactivity is strongly associated with indices of drug use and other individual-specific factors. Future studies should focus on high quality studies to support evidence-based interventions for reducing cue reactivity and to examine cue reactivity as an outcome measure.
Female Human Papillomavirus Infection Associated with Increased Risk of Infertility: A Nationwide Population-Based Cohort Study
Authors: Hsu Li-Chuan , Tsui Kuan-Hao , Wei James Cheng-Chung , Yip Hei-Tung , Hung Yao-Min , Chang Renin
Published: September 2020

Objective. This study investigated whether women with a history of human papillomavirus (HPV) infection have an increased risk of infertility. Material and Methods. All patients with an HPV infection (n = 11,198) in Taiwan’s National Health Insurance Research Database (2000–2012) were propensity score matched with control subjects (n = 11,198) without an HPV infection by age, sex, index year, and relevant co-morbidities. Both groups were tracked until a diagnosis of infertility was recorded. The Chi-square test was used to analyze the distribution of demographic characteristics in the HPV group and non-HPV group. A Cox proportional hazards regression was used to estimate the hazard ratios (HRs) for the development of infertility, adjusting for age, sex, and co-morbidities. The Kaplan–Meier method was used to plot the cumulative incidence curves. We also performed negative controls to test for possible unmeasured confounding. Results. The HPV cohort had a higher risk of infertility. The adjusted HR (aHR) was found to be 1.39 (95% CI = 1.19–1.63) after adjusting for demographic characteristics and relevant co-morbidities. In the age subgroup analysis, patients with an HPV infection had an increased risk of infertility compared to the non-HPV cohort in the group aged 26 to 35 years (aHR, 1.53; 95% CI = 1.24–1.88). As we used propensity score matching to treat measurable confounders and negative controls to access unmeasured confounders, the findings of the study are robust. Conclusions. Among females of reproductive age, HPV infection is a potential risk factor that predisposes individuals to subsequent infertility.
Assessment of Interventions in Fuel Management Zones Using Remote Sensing
Authors: Afonso Ricardo , Neves André , Viegas Damásio Carlos , Moura Pires João , Birra Fernando , Santos Maribel Yasmina
Published: September 2020

Every year, wildfires strike the Portuguese territory and are a concern for public entities and the population. To prevent a wildfire progression and minimize its impact, Fuel Management Zones (FMZs) have been stipulated, by law, around buildings, settlements, along national roads, and other infrastructures. FMZs require monitoring of the vegetation condition to promptly proceed with the maintenance and cleaning of these zones. To improve FMZ monitoring, this paper proposes the use of satellite images, such as the Sentinel-1 and Sentinel-2, along with vegetation indices and extracted temporal characteristics (max, min, mean and standard deviation) associated with the vegetation within and outside the FMZs and to determine if they were treated. These characteristics feed machine-learning algorithms, such as XGBoost, Support Vector Machines, K-nearest neighbors and Random Forest. The results show that it is possible to detect an intervention in an FMZ with high accuracy, namely with an F1-score ranging from 90% up to 94% and a Kappa ranging from 0.80 up to 0.89.
FLASH Radiotherapy: Current Knowledge and Future Insights Using Proton-Beam Therapy
Authors: Hughes Jonathan R. , Parsons Jason L.
Published: September 2020

FLASH radiotherapy is the delivery of ultra-high dose rate radiation several orders of magnitude higher than what is currently used in conventional clinical radiotherapy, and has the potential to revolutionize the future of cancer treatment. FLASH radiotherapy induces a phenomenon known as the FLASH effect, whereby the ultra-high dose rate radiation reduces the normal tissue toxicities commonly associated with conventional radiotherapy, while still maintaining local tumor control. The underlying mechanism(s) responsible for the FLASH effect are yet to be fully elucidated, but a prominent role for oxygen tension and reactive oxygen species production is the most current valid hypothesis. The FLASH effect has been confirmed in many studies in recent years, both in vitro and in vivo, with even the first patient with T-cell cutaneous lymphoma being treated using FLASH radiotherapy. However, most of the studies into FLASH radiotherapy have used electron beams that have low tissue penetration, which presents a limitation for translation into clinical practice. A promising alternate FLASH delivery method is via proton beam therapy, as the dose can be deposited deeper within the tissue. However, studies into FLASH protons are currently sparse. This review will summarize FLASH radiotherapy research conducted to date and the current theories explaining the FLASH effect, with an emphasis on the future potential for FLASH proton beam therapy.
Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
Authors: Lu Xiaocen , Shen Yi , Campbell Robert E.
Published: September 2020

Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl).
Transitions and Instabilities in Imperfect Ion-Selective Membranes
Authors: Schiffbauer Jarrod , Demekhin Evgeny , Ganchenko Georgy
Published: September 2020

Numerical investigation of the underlimiting, limiting, and overlimiting current modes and their transitions in imperfect ion-selective membranes with fluid flow through permitted through the membrane is presented. The system is treated as a three layer composite system of electrolyte-porous membrane-electrolyte where the Nernst–Planck–Poisson–Stokes system of equations is used in the electrolyte, and the Darcy–Brinkman approach is employed in the nanoporous membrane. In order to resolve thin Debye and Darcy layers, quasi-spectral methods are applied using Chebyshev polynomials for their accumulation of zeros and, hence, best resolution in the layers. The boundary between underlimiting and overlimiting current regimes is subject of linear stability analysis, where the transition to overlimiting current is assumed due to the electrokinetic instability of the one-dimensional quiescent state. However, the well-developed overlimiting current is inherently a problem of nonlinear stability and is subject of the direct numerical simulation of the full system of equations. Both high and low fixed charge density membranes (low- and high concentration electrolyte solutions), acting respectively as (nearly) perfect or imperfect membranes, are considered. The perfect membrane is adequately described by a one-layer model while the imperfect membrane has a more sophisticated response. In particular, the direct transition from underlimiting to overlimiting currents, bypassing the limiting currents, is found to be possible for imperfect membranes (high-concentration electrolyte). The transition to the overlimiting currents for the low-concentration electrolyte solutions is monotonic, while for the high-concentration solutions it is oscillatory. Despite the fact that velocities in the porous membrane are much smaller than in the electrolyte region, it is further demonstrated that they can dramatically influence the nature and transition to the overlimiting regimes. A map of the bifurcations, transitions, and regimes is constructed in coordinates of the fixed membrane charge and the Darcy number.
Characterization of Insulin-Like Growth Factor Binding Protein 7 (Igfbp7) and Its Potential Involvement in Shell Formation and Metamorphosis of Pacific Abalone, Haliotis discus hannai
Authors: Sharker Md. Rajib , Hossen Shaharior , Nou Ill-Sup , Kho Kang Hee
Published: September 2020

Insulin-like growth factor binding proteins (IGFBPs) are secreted proteins that play an important role in IGF regulation of growth and development of vertebrate and invertebrates. In this study, the IGFBP7 gene was cloned and characterized from mantle tissues of H. discus hannai, and designated as Hdh IGFBP7. The full-length cDNA sequence transcribed from the Hdh IGFBP7 gene was 1519-bp long with an open reading frame of 720-bp corresponding to a putative polypeptide of 239 amino acids. The molecular mass of its mature protein was approximately 23.44 KDa with an estimated isoelectric point (pI) of 5.35, and it shared significant homology with IGFBP7 gene of H. madaka. Hdh IGFBP7 has a characteristic IGFBP N-terminal domain (22–89 aa), a kazal-type serine proteinase inhibitor domain (77–128), and an immunoglobulin-like C2 domain (144–223). Furthermore, twelve cysteine residues and a signature motif of IGFBPs (XCGCCXXC) were found in its N-terminal domain. Phylogenetic analysis revealed that Hdh IGFBP7 was aligned with IGFBP7 of H. madaka. Tissue distribution analysis showed that the mRNA of Hdh IGFBP7 was expressed in all examined tissues, with the highest expression level observed in the mantle and gill tissues. The expression level of Hdh IGFBP7 mRNA was relatively higher at the juvenile stage during its metamorphosis period. In situ hybridization showed that Hdh IGFBP7 transcript was expressed in epithelial cells of the dorsal mantle pallial and mucus cells of the branchial epithelium in gill. These results provide basic information for future studies on the role of IGFBP7 in IGF regulation of shell growth, development and metamorphosis of abalone.
Bacterial Cellulose—Graphene Based Nanocomposites
Authors: Troncoso Omar P. , Torres Fernando G.
Published: September 2020

Bacterial cellulose (BC) and graphene are materials that have attracted the attention of researchers due to their outstanding properties. BC is a nanostructured 3D network of pure and highly crystalline cellulose nanofibres that can act as a host matrix for the incorporation of other nano-sized materials. Graphene features high mechanical properties, thermal and electric conductivity and specific surface area. In this paper we review the most recent studies regarding the development of novel BC-graphene nanocomposites that take advantage of the exceptional properties of BC and graphene. The most important applications of these novel BC-graphene nanocomposites include the development of novel electric conductive materials and energy storage devices, the preparation of aerogels and membranes with very high specific area as sorbent materials for the removal of oil and metal ions from water and a variety of biomedical applications, such as tissue engineering and drug delivery. The main properties of these BC-graphene nanocomposites associated with these applications, such as electric conductivity, biocompatibility and specific surface area, are systematically presented together with the processing routes used to fabricate such nanocomposites.
Mechanisms for Curing Yeast Prions
Authors: Greene Lois E. , Saba Farrin , Silberman Rebecca E. , Zhao Xiaohong
Published: September 2020

Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded prion proteins are assembled into amyloid fibers or seeds, which are constantly severed and transmitted to daughter cells. To cure prions in yeast, it is necessary to eliminate all the prion seeds. Multiple mechanisms of curing have been found including inhibiting severing of the prion seeds, gradual dissolution of the prion seeds, asymmetric segregation of the prion seeds between mother and daughter cells during cell division, and degradation of the prion seeds. These mechanisms, achieved by using different protein quality control machinery, are not mutually exclusive; depending on conditions, multiple mechanisms may work simultaneously to achieve curing. This review discusses the various methods that have been used to differentiate between these mechanisms of curing.
Maize Bushy Stunt Phytoplasma Favors Its Spread by Changing Host Preference of the Insect Vector
Authors: Ramos Anderson , Esteves Mariana Bossi , Cortés Mayerli Tatiana Borbón , Lopes João Roberto Spotti
Published: September 2020

Plant pathogenic bacteria may influence vector behavior by inducing physiological changes in host plants, with implications for their spread. Here, we studied the effects of maize bushy stunt phytoplasma (MBSP) on the host selection behavior of the leafhopper vector, Dalbulus maidis (DeLong and Wolcott). Choice assays contrasting leaves of healthy (mock-inoculated) vs. infected maize (Zea mays L.) were conducted during the asymptomatic and symptomatic phases of plant infection, with leafhopper males or females previously exposed to infected plants (bacteriliferous insects) or not. In each assay, 40 adults were released in choice arenas where only the leaves of two plants from each treatment were offered and visible, and the insects landed on the leaves were counted 1, 2, 3, 5, 7, 9, 11 and 23 h after release. During the asymptomatic phase of plant infection, an effect was observed only on bacteriliferous females, who preferred leaves of healthy plants 5 h after release or later. The symptomatic phase triggered a pull–push effect on non-bacteriliferous females, who were first attracted to symptomatic leaves but hours later moved to healthy leaves. Non-bacteriliferous males initially preferred symptomatic leaves (up to 5 h after release) and later became equally distributed between treatments. Bacteriliferous males and females initially did not discriminate between healthy and symptomatic leaves, but only the females tended to move to healthy leaves 9 h after release. Oviposition was drastically reduced on symptomatic leaves. The changes in vector behavior induced by MBSP favor its primary spread, since bacteriliferous females prefer healthy leaves at early (asymptomatic) stages of the crop. At later stages, secondary spread may be favored because non-bacteriliferous females are initially attracted to infected (symptomatic) leaves, allowing pathogen acquisition and subsequent transmission as they move to healthy plants.
Knockdown of the Trehalose-6-Phosphate Synthase Gene Using RNA Interference Inhibits Synthesis of Trehalose and Increases Lethality Rate in Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae)
Authors: Liu Xinyu , Zou Zhiwen , Zhang Cong , Liu Xian , Wang Jing , Xin Tianrong , Xia Bin
Published: September 2020

Diaphorina citri Kuwayama is the vector of citrus “huanglongbing”, a citrus disease which poses a significant threat to the global citrus industry. Trehalose-6-phosphate synthase (TPS) plays an important role in the regulation of trehalose levels of insects, while its functions in D. citri are unclear. In this study, full-length cDNA sequences of the TPS gene from D. citri (DcTPS) were cloned and its expression patterns at various developmental stages were investigated. The results indicated that DcTPS mRNA was expressed at each developmental stage and the highest DcTPS expression was found in the fifth-instar nymphs of D. citri. Additionally, mortality and deformity of D. citri were observed after 24 and 48 h by feeding with three different dsRNA concentrations (20, 100 and 500 ng/μL). The results indicated that DcTPS expression was declined, and mortality and malformation in nymphs were increased via feeding with dsDcTPS. Moreover, the enzyme and trehalose content were decreased, while the content of glucose was significantly higher than that of untreated (control) individuals. This suggests that DcTPS might be vital for the growth and development of D. citri and further studies of the genes should be related to molting and metabolism for controlling D. citri.
Triage of Amyotrophic Lateral Sclerosis Patients during the COVID-19 Pandemic: An Application of the D50 Model
Authors: Steinbach Robert , Prell Tino , Gaur Nayana , Stubendorff Beatrice , Roediger Annekathrin , Ilse Benjamin , Witte Otto , Grosskreutz Julian
Published: September 2020

Amyotrophic lateral sclerosis (ALS) is a progressive neuromuscular disease, the management of which requires the continuous provision of multidisciplinary therapies. Owing to the novel coronavirus disease (COVID-19) pandemic, regular contact with ALS patients at our center was severely restricted and patient care was at risk by delay of supportive therapies. We established a triage system based on the D50 disease progression model and were thus able to identify a prospective cohort with high disease aggressiveness (D50 < 30). Thirty-seven patients with highly aggressive disease were actively offered follow-up, either via telephone or on-site, depending on their disease-specific needs and abilities. We describe here the procedures, obstacles, and results of these prescient efforts during the restrictions caused by COVID-19 in the period between March and June 2020. In conclusion, four patients with highly aggressive disease were initiated with non-invasive ventilation and two received a gastrostomy. We could show that a comparable amount of advanced care was induced in a retrospective cohort within a similar time period one year prior to the COVID-19 outbreak. Our workflow to identify high-risk patients via D50 model metrics can be easily implemented and integrated within existing centers. It helped to maintain a high quality of advanced care planning for our ALS patients.
Effects of Topical Prostaglandin Analog on Macular Thickness Following Cataract Surgery with Postoperative Topical Bromfenac Treatment
Authors: Park Kee Sup , Kim Kyoung Nam , Kim Kyeung Min , Lee Han Min , Lee Sung Bok , Lee Nam Ho , Kim Chang-Sik
Published: September 2020

Purpose: To evaluate changes in macular thickness in patients continuing prostaglandin analog (PGA) treatment during the perioperative period involving bromfenac treatment. Methods: Patients with glaucoma who were using a topical PGA were randomly assigned to two groups in this randomized controlled trial: PGA continuing study group and PGA discontinued glaucoma control group. Patients without ocular diseases other than cataract were enrolled into the non-glaucomatous group. After the cataract surgery, the patients used bromfenac twice per day for 4 weeks. Optical coherence tomography was performed in all patients preoperatively and at 1 month postoperatively. Changes in macular thickness were compared among the three groups. Results: There were 32 eyes in the study group, 33 eyes in the glaucoma control group, and 58 eyes in the non-glaucomatous group. We found statistically significant postoperative changes in central macular thickness in all groups (4.30 ± 8.01 μm in the PGA continuing group, 9.20 ± 13.88 μm in the PGA discontinued group, and 7.06 ± 7.02 μm in the non-glaucomatous group, all p < 0.008), but no significant difference among the three groups (p = 0.161). Cystoid macular edema occurred in only one patient in the non-glaucomatous group (p = 0.568). Conclusions: Continuous use of PGAs during the perioperative period was not significantly associated with increased macular thickness after uncomplicated cataract surgery. In the absence of other risk factors (e.g., capsular rupture, uveitis, or diabetic retinopathy), discontinuing PGAs for the prevention of macular edema after cataract surgery with postoperative bromfenac treatment is unnecessary in patients with glaucoma.
Systemic Inflammatory Response and Outcomes in Community-Acquired Pneumonia Patients Categorized According to the Smoking Habit or Presence of Chronic Obstructive Pulmonary Disease
Authors: Crisafulli Ernesto , Cillóniz Catia , Liapikou Adamantia , Ferrari Marcello , Busti Fabiana , Girelli Domenico , Torres Antoni
Published: September 2020

The systemic inflammatory response (SIR) may help to predict clinical progression, treatment failure, and prognosis in community-acquired pneumonia (CAP). Exposure to tobacco smoke may affect the SIR; the role of smoking in CAP has not been consolidated. We evaluated the SIR and outcomes of hospitalized CAP patients stratified by smoking habits and the presence of COPD. This retrospective analysis was conducted at the Hospital Clinic of Barcelona. Baseline, clinical, microbiological, and laboratory variables were collected at admission, using C-reactive protein (CRP) levels as a marker of SIR. The study outcomes were pleural complications, hospital stay, non-invasive and invasive mechanical ventilation (IMV), and intensive care unit (ICU) admission. We also considered the in-hospital and 30-day mortality. Data were grouped by smoking habit (non-, former-, and current-smokers) and the presence of COPD. Current smokers were younger, had fewer comorbidities, and fewer previous pneumonia episodes. CRP levels were higher in current smokers than in other groups. Current smokers had a higher risk of pleural complications independent of CRP levels, the presence of pleuritic pain, and a higher platelet count. Current smokers more often required IMV and ICU admission. Current smokers have a greater inflammatory response and are at increased risk of pleural complications.
Characterization of Thrombin Generation Curve Shape in Presence of Platelets from Acute Venous Thromboembolism Patients
Authors: Lagrange Jeremy , Wagner Bianca , Nagler Markus , ten Cate Vincent , Pallares Robles Alejandro , Koeck Thomas , Rapp Steffen , Prochaska Jürgen H. , Spronk Henri M. , Wenzel Philip , Ruf Wolfram , ten Cate Hugo , Wild Philipp S. , Panova-Noeva Marina
Published: September 2020

Background. Anticoagulant therapy, the cornerstone treatment in acute venous thromboembolism (VTE), strongly impacts thrombin generation (TG). Until now, the appearance of the TG curve in platelet rich plasma (PRP) from patients with acute VTE has not been investigated. Methods. We analyzed the shape of TG curves measured in PARP of 180 acute VTE patients. Results. Normal shape of TG curves was observed in 110 patients, 50 patients showed no TG and 20 patients showed biphasic TG curve. The linear regression analysis, adjusted for age, sex, VTE clinical phenotypes and therapy showed that the appearance of biphasic curves is significantly associated with female sex, presence of cancer and therapy with Factor Xa inhibitors. Conclusions. This study demonstrated that despite taking anticoagulants, TG in presence of platelets is still present in the majority of acute VTE patients. Appearance of unusual TG curves is strongly related to the intake of anti-Factor Xa inhibitors. The clinical relevance of biphasic TG curve appearance requires further investigation.
Piezoresistive and mechanical Behavior of CNT based polyurethane foam
Authors: De Meo Enea , Agnelli Simone , Veca Antonino , Brunella Valentia , Zanetti Marco
Published: September 2020

Carbon nanotubes (CNT) embedded into a polymeric foam demonstrate an enhancement in electrical and mechanical properties of the final nanocomposite. The enhanced material with new characteristics, e.g., piezoresistivity, can be substituted with the traditional metallic material to design sensors, switches, and knobs directly into a single multifunctional component. Research activities in this field are moving towards a mono-material fully integrated smarts components. In order to achieve this goal, a simple method is developed to produce piezoresistive polyurethane/CNT foams. The novelty consists in applying the dispersion of CNT considering industrial production constrains, in order to facilitate its introduction into a common industrial practice. Three kinds of PU-CNT foam have been prepared and tested: PU-CNT 1.5%, PU-CNT-COOH 1.0%, and PU-CNT-COOH 1.5%. Polyurethane with CNT-COOH showed an insulating-conductive transition phenomenon when the foam reaches the 80% of its compression strain with a Gauge factor (Gf) of about 30. Instead, PU-CNT showed conductivity only at 1.5% of filler concentration and a steady piezoresistive behavior with a Gf of 80. However, this samples did not show the insulating-conductive transition. Having improved the electromechanical properties of final nanocomposite polyurethane foam demonstrates that the proposed method can be applied differently for design sensors and switches.
A Series of Tubes: The C. elegans Excretory Canal Cell as a Model for Tubule Development
Authors: Buechner Matthew , Yang Zhe , Al-Hashimi Hikmat
Published: September 2020

Formation and regulation of properly sized epithelial tubes is essential for multicellular life. The excretory canal cell of C. elegans provides a powerful model for investigating the integration of the cytoskeleton, intracellular transport, and organismal physiology to regulate the developmental processes of tube extension, lumen formation, and lumen diameter regulation in a narrow single cell. Multiple studies have provided new understanding of actin and intermediate filament cytoskeletal elements, vesicle transport, and the role of vacuolar ATPase in determining tube size. Most of the genes discovered have clear homologues in humans, with implications for understanding these processes in mammalian tissues such as Schwann cells, renal tubules, and brain vasculature. The results of several new genetic screens are described that provide a host of new targets for future studies in this informative structure.
Reconfiguring Passenger Ship Internal Environment for Damage Stability Enhancement
Authors: Vassalos Dracos , Paterson Donald
Published: September 2020

The traditional risk control option adopted in naval architecture to meet safety-related objectives is by regulations, targeting damage limitation, nominally instigated in the wake of maritime accidents claiming heavy loss of life. These primarily concern the introduction of watertight bulkheads, i.e., permanent (passive) reconfiguration of the internal ship environment to enhance damage stability. This has been the most common measure, manifesting itself in the wake of every serious flooding accident since the beginning, back in the 19th century. However, traditional flooding protection through watertight subdivision, to an extent dictated by IMO regulations, has a physical limit which, if exceeded, a safety plateau is reached. This is currently the case and with damage stability standards progressively increasing, the safety gap between existing and new ships is dangerously widening and with design stability margins progressively eroding, stability management is unsustainable, leading to loss of earnings at best. The need for managing the residual risk through active intervention/protection over the life-cycle of the vessel drives industry to searching and adopting a new normal. This new normal is the innovation being explained in this paper by addressing safety enchantment through a systematic reconfiguration of the ship environment for passive and active protection in flooding accidents. In this respect, the “design-optimal” internal arrangement of a vessel, is adapted and reconfigured, using passive and active containment systems for flooding incidents, in the form of high-expansion foam products. The innovation is briefly explained, claiming transformational reduction in flooding risk in the most cost-effective way available. To support wider understanding and appreciation for the latter, the paper critically reviews the whole evolution of internal ship space reconfiguration, chronologically and systematically, concluding that new technological developments and breakthroughs will bring sustainable changes to the traditional evolutionary maritime safety enhancement.
Pan-Echinocandin-Resistant Candida glabrata Bloodstream Infection Complicating COVID-19: A Fatal Case Report
Authors: Posteraro Brunella , Torelli Riccardo , Vella Antonietta , Leone Paolo Maria , De Angelis Giulia , De Carolis Elena , Ventura Giulio , Sanguinetti Maurizio , Fantoni Massimo
Published: September 2020

Coinfections with bacteria or fungi may be a frequent complication of COVID-19, but coinfections with Candida species in COVID-19 patients remain rare. We report the 53-day clinical course of a complicated type-2 diabetes patient diagnosed with COVID-19, who developed bloodstream infections initially due to methicillin-resistant Staphylococcus aureus, secondly due to multidrug-resistant Gram-negative bacteria, and lastly due to a possibly fatal Candida glabrata. The development of FKS-associated pan-echinocandin resistance in the C. glabrata isolated from the patient after 13 days of caspofungin treatment aggravated the situation. The patient died of septic shock shortly before the prospect of receiving potentially effective antifungal therapy. This case emphasizes the importance of early diagnosis and monitoring for antimicrobial drug-resistant coinfections to reduce their unfavorable outcomes in COVID-19 patients.