bluuebunny/crossref_metadata_2025 huggingface dataset:
Blood Urea Nitrogen Is Associated with In-Hospital Mortality in Critically Ill Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease: A Propensity Score Matching Analysis
Authors: Giri Mohan , He Lin , Hu Tianyang , Puri Anju , Zheng Xiaozhuo , Dai Haiyun , Guo Shuliang
Published: November 2022

Background: Elevated blood urea nitrogen (BUN) level is associated with a higher risk of mortality in various diseases; however, the association between BUN level and in-hospital mortality in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) admitted to the intensive care unit (ICU) is not known. This study aimed to investigate the relationship between BUN level and in-hospital mortality in patients with AECOPD admitted to the ICU. Methods: In this retrospective cohort study, AECOPD patients were identified from the Medical Information Mart for Intensive Care (MIMIC-IV) database. Multivariate regression was used to elucidate the relationship between BUN level and in-hospital mortality, and propensity score matching (PSM) was used to adjust confounders. Receiver operating characteristics and Kaplan–Meier curves were used to evaluate the relationship between BUN level and in-hospital mortality. Results: Data from 1201 patients were analyzed. The all-cause in-hospital mortality was 13.7%. BUN levels were significantly higher in non-survivors compared to the survival group before (p < 0.001) and after (p = 0.005) PSM. Multivariate analysis indicated that elevated BUN levels were independently associated with increased risk of in-hospital mortality both before (p = 0.002) and after (p = 0.015) PSM. The optimal BUN cut-off value for in-hospital mortality in critical patients with AECOPD before (>23 mg/dL) and after (>22 mg/dL) PSM was comparable. Compared with the low BUN group, the hazard ratio (HR) of the high BUN group was 1.8987 (before PSM) and 1.7358 (after PSM). Conclusions: Higher BUN levels were significantly associated with an increased risk of in-hospital mortality in critically ill patients with AECOPD. As a widely available and rapidly measured biomarker, BUN may be useful in the risk stratification of critically ill AECOPD patients. The results need to be verified in prospective studies.
A New Pharmacological Vitreolysis through the Supplement of Mixed Fruit Enzymes for Patients with Ocular Floaters or Vitreous Hemorrhage-Induced Floaters
Authors: Ma Jui-Wen , Hung Jen-Lin , Takeuchi Masaru , Shieh Po-Chuen , Horng Chi-Ting
Published: November 2022

Purpose: Ocular floaters caused by vitreous degeneration or blood clots may interfere with various visual functions. Our study investigated the pharmacologic effects of oral supplementation of mixed fruit enzymes (MFEs) for treating spontaneous symptomatic vitreous opacities (SVOs) and those secondary to vitreous hemorrhage (VH). Methods: 224 patients with monocular symptomatic vitreous opacities (SVOs) were recruited between September and December 2017 and received oral supplementation of MFEs (190 mg bromelain, 95 mg papain, and 95 mg ficin) for 3 months in a double-blind clinical trial. Participants were divided according to the etiology of the SVOs, spontaneous (experiment 1) versus VH (experiment 2), and then randomly assigned into four treatments groups: one group received oral vitamin C, as a placebo; and the other 3 groups received 1 capsule per day (low dose), 2 capsules per day (middle dose), or 3 capsules per day (high dose) of MFEs. The number of SVOs was determined at baseline and then 1, 2, and 3 months after initiating treatment. Further, in cases secondary to VH, the changes in corrected distance visual acuity (CDVA) were assessed after 3 months. Second, we compared the free radical scavenging capabilities of each substance: vitamin C, bromelain, papain, ficin, and MFEs (combination of bromelain, papain, and ficin) by DDPH assay. Finally, SVOs-related symptoms and satisfaction with the treatments were evaluated at the last follow-up visit Results: In experiment 1, the disappearance rate of SVOs was 55%, 62.5%, and 70% after taking 1, 2, and 3 capsules daily, respectively (total p < 0.001), in a dose-dependent manner. In experiment 2, the disappearance rate of VH-induced SVOs was 18%, 25%, and 56% (p < 0.001) after 1, 2, and 3 capsules of the supplement daily, respectively. Additionally, the patients’ vision elevated from 0.63LogMAR to 0.19LogMAR (p = 0.008). Conclusions: A pharmacological approach using a high dose of oral supplementation with MFEs (bromelain, papain, and ficin) was effective in reducing vitreous opacities, even after intraocular hemorrhage. Furthermore, pharmacologic vitreolysis with MFEs supplementation showed high patient satisfaction, and also improved CDVA in patients with vitreous hemorrhage-induced floaters
Are There Similarities in Pregnancy Complications and Delivery Outcomes among Sisters?
Authors: Shalev-Ram Hila , Cohen Gal , Ram Shai , Heresco Lior , Schreiber Hanoch , Biron-Shental Tal , Kovo Michal , Ravid Dorit
Published: November 2022

This retrospective cohort study evaluated pregnancy outcomes and similarities between pairs of nulliparous sisters with a singleton fetus who delivered between 2013 and 2020. The “Sister-1 group” was defined as the sibling who delivered first, while the “Sister-2 group” included the siblings who gave birth after Sister-1. Obstetrical complications and delivery outcomes were compared. The relative risk for recurrence of a complication in Sister-2 was calculated. The study included 743 sister pairs. There were no between-group differences in maternal BMI, gestational age at delivery, gravidity, smoking, or epidural rates. The Sister-2 group was older than the Sister-1 group (26.4 ± 5 vs. 25.8 ± 4.7 years, respectively, p = 0.05). Higher birthweights and more large-for-gestational-age infants characterized the Sister-2 group compared with the Sister-1 group (3241 ± 485 g vs. 3148 ± 536 g, p < 0.001 and 7.7% vs. 4.8%, p = 0.025, respectively). There were no between-group differences in the rate of small-for-gestational-age, gestational diabetes, hypertensive disorders, pre-term births, vacuum extraction, or cesarean deliveries. Logistic regression analysis found that if Sister-1 underwent vacuum extraction, her sibling had an increased risk for vacuum delivery (adjusted RR 3.03, 95% CI 1.4–6.7; p = 0.003) compared with those whose sibling (Sister-1) did not. There was a three-fold risk of vacuum extraction delivery between sisters. This finding could be related to biological inheritance, environmental factors, and/or psychological issues that may affect similarities between siblings’ delivery outcomes.
Systemic Lupus Erythematosus and Lung Involvement: A Comprehensive Review
Authors: Shin Jae Il , Lee Keum Hwa , Park Seoyeon , Yang Jae Won , Kim Hyung Ju , Song Kwanhyuk , Lee Seungyeon , Na Hyeyoung , Jang Yong Jun , Nam Ju Yun , Kim Soojin , Lee Chaehyun , Hong Chanhee , Kim Chohwan , Kim Minhyuk , Choi Uichang , Seo Jaeho , Jin Hyunsoo , Yi BoMi , Jeong Se Jin , Sheok Yeon Ook , Kim Haedong , Lee Sangmin , Lee Sangwon , Jeong Young Soo , Park Se Jin , Kim Ji Hong , Kronbichler Andreas
Published: November 2022

Systemic lupus erythematosus (SLE) is a complex autoimmune disease with multiorgan manifestations, including pleuropulmonary involvement (20–90%). The precise mechanism of pleuropulmonary involvement in SLE is not well-understood; however, systemic type 1 interferons, circulating immune complexes, and neutrophils seem to play essential roles. There are eight types of pleuropulmonary involvement: lupus pleuritis, pleural effusion, acute lupus pneumonitis, shrinking lung syndrome, interstitial lung disease, diffuse alveolar hemorrhage (DAH), pulmonary arterial hypertension, and pulmonary embolism. DAH has a high mortality rate (68–75%). The diagnostic tools for pleuropulmonary involvement in SLE include chest X-ray (CXR), computed tomography (CT), pulmonary function tests (PFT), bronchoalveolar lavage, biopsy, technetium-99m hexamethylprophylene amine oxime perfusion scan, and (18)F-fluorodeoxyglucose positron emission tomography. An approach for detecting pleuropulmonary involvement in SLE includes high-resolution CT, CXR, and PFT. Little is known about specific therapies for pleuropulmonary involvement in SLE. However, immunosuppressive therapies such as corticosteroids and cyclophosphamide are generally used. Rituximab has also been successfully used in three of the eight pleuropulmonary involvement forms: lupus pleuritis, acute lupus pneumonitis, and shrinking lung syndrome. Pleuropulmonary manifestations are part of the clinical criteria for SLE diagnosis. However, no review article has focused on the involvement of pleuropulmonary disease in SLE. Therefore, this article summarizes the literature on the epidemiology, pathogenesis, diagnosis, and management of pleuropulmonary involvement in SLE.
The Addition of Diacutaneous Fibrolysis to a Pharmacological Intervention in Patients with Tension-Type Headache: A Randomized Controlled Trial
Authors: Cabanillas-Barea Sara , Ceballos-Laita Luis , Pérez-Guillén Silvia , Jiménez-del-Barrio Sandra , Pardos-Aguilella Pilar , Rodríguez-Rubio Pere Ramón , Carrasco-Uribarren Andoni
Published: November 2022

Background: Tension-type headache (TTH) is the most common headache worldwide. Pharmacological interventions are the most investigated therapies in patients with TTH. The addition of physical therapy treatments such as diacutaneous fibrolysis (DF) may have promising results. The aim of this study was to investigate the addition of three sessions of DF to a pharmacological intervention in patients with TTH. Methods: A single-blinded randomized controlled trial was carried out. Participants were randomly assigned to the standard care group or to the DF group. Both groups received a pharmacological intervention. Three sessions of DF in the thoracic and cervicocranial region were added in the DF group. The impact caused by headache (HIT-6), headache intensity, and cervical range of motion were measured by blinded assessors at baseline, after the intervention, and at 1 month follow-up. Results: Eighty-two patients with TTH were included (41 standard care group; 41 DF group). Statistically significant differences were found between both groups in all the variables after the intervention and at 1 month follow-up (p < 0.001). No adverse effects or side-effects were reported during the study. Conclusions: The addition of three sessions of DF to a pharmacological therapy provided improvements in the impact caused by headache, headache intensity, and cervical range of motion after the intervention and at 1 month follow-up compared to a pharmacological therapy in isolation. Further research is needed to investigate long-term effects.
Comparative Effectiveness Research: A Roadmap to Sail the Seas of IBD Therapies
Authors: Pugliese Daniela , Onali Sara , Privitera Giuseppe , Armuzzi Alessandro , Papi Claudio
Published: November 2022

The drug pipeline for the treatment of inflammatory bowel disease (IBD) has dramatically expanded over the last two decades, and it is expected to further grow in the upcoming years with the introduction of new agents with different mechanisms of action. However, such an increase of therapeutic options needs to be paralleled with an appropriate development of research to help physicians in the decision-making process when choosing which drug to prescribe. On the population level, comparative effectiveness research (CER) is intended to explore and identify relevant differences—in terms of both efficacy and safety outcomes—amongst different therapeutic regimens and/or strategies, in order to find the correct placement for each treatment in the therapeutic algorithm. CER revolves around three cornerstones: network meta-analyses, head-to-head trials and real-world studies, each of which has specific pros and cons, and can therefore offer answers to different questions. In this review, we aim to provide an overview on the methodological features specific to each of these research approaches, as well as to illustrate the main findings coming from CER on IBD target therapies (i.e., biologics and small molecules) and to discuss their appropriate interpretation.
Validation of Childhood Rare Epilepsy Social Impact Assessment (CRESIA) to Measure the Social and Family Impact of Rare Childhood Diseases with Epilepsy
Authors: Salom Rafael , Aras Luis Miguel , Piñero Jessica , Duñabeitia Jon Andoni
Published: November 2022

This study addresses the social relevance of low-prevalence childhood diseases and reports the process of generation and validation of a tool to assess the social impact on the direct family environment and the social context of reference. The aim of the process of construction and validation of this instrument is to provide the field with a tool with the capacity to shed light on the social consequences of suffering from a low-prevalence disease, specifically those comorbid with treatment-resistant epileptic seizures of childhood origin. The instrument here presented and called CRESIA (acronym derived from Childhood Rare Epilepsy Social Impact Assessment) provides valuable information on six specific areas framing health, economic, psychological, social, and child-related stressors, as well as family. CRESIA represents a valid and reliable instrument for family members or primary caregivers of children and adolescents with childhood rare epilepsy.
Deep Endometriosis and Infertility: What Is the Impact of Surgery?
Authors: Daniilidis Angelos , Angioni Stefano , Di Michele Stefano , Dinas Konstantinos , Gkrozou Fani , D’Alterio Maurizio Nicola
Published: November 2022

In women with deep endometriosis, the spontaneous fertility rate might range from 2 to 10%. The optimal management of these women is still an area of debate. Therefore, this review aims to explore the literature on the impact of deep endometriosis surgery on reproductive outcomes and pregnancy rates in women with and without prior infertility. A total of 392 articles were identified through database searching. Twenty-three studies were eligible to be included in the review. A total of 1548 women were identified, 814 of whom became pregnant, with a mean pregnancy rate of 52.6% (95% CI 49.7–63%). Our review suggests that surgery may improve fertility outcomes. Due to the variability in the studies, it is impossible to stratify fertility outcomes of surgery by the localization of deep endometriosis. More investigations are needed to determine whether surgical management should be first-intention or limited to the failure of medically assisted reproduction treatment.
Stop Worrying about Multiple-Choice: Fact Knowledge Does Not Change with Response Format
Authors: Goecke Benjamin , Staab Marlena , Schittenhelm Catherine , Wilhelm Oliver
Published: November 2022

Declarative fact knowledge is a key component of crystallized intelligence. It is typically measured with multiple-choice (MC) items. Other response formats, such as open-ended formats are less frequently used, although these formats might be superior for measuring crystallized intelligence. Whereas MC formats presumably only require recognizing the correct response to a question, open-ended formats supposedly require cognitive processes such as searching for, retrieving, and actively deciding on a response from long-term memory. If the methods of inquiry alter the cognitive processes involved, mean-changes between methods for assessing declarative knowledge should come along with changes in the covariance structure. We tested these assumptions in two online studies administering declarative knowledge items in different response formats (MC, open-ended, and open-ended with cues). Item difficulty clearly increases in the open-ended methods although effects in logistic regression models vary slightly across items. Importantly, latent variable analyses suggest that the method of inquiry does not affect what is measured with different response formats. These findings clearly endorse the position that crystallized intelligence does not change as a function of the response format.
Ultra-Low-Power Circuits for Intermittent Communication
Authors: Torrisi Alessandro , Yıldırım Kasım Sinan , Brunelli Davide
Published: November 2022

Self-sustainable energy harvesting for Internet of Things devices is challenging since ambient energy may be sporadic and unpredictable. This situation leads to frequent power failures that lead to intermittent operations, which prevent the reliability of data communications. This article presents fundamental hardware circuitry that enables reliable intermittent communications over wireless batteryless node networks. We emphasize two main mechanisms that ensure energy awareness and reliability: energy status-sharing and synchronized operation. We introduce novel low-power and self-sustainable plug-and-play circuits to support these mechanisms.
Sedimentological Analysis of Regional Differentiation and Sediment Provenance in the Lu’erhuan River Sea Area of Qinzhou Bay, Guangxi Province
Authors: Li Ping , Du Jun , Zhang Zhiwei , Xu Guoqiang
Published: November 2022

Globally, coastal regions are vital areas of human activity and, as such, are centers of population growth and urban and economic development. Long-term human development has had a major impact on the ecological environment of coastal zones. Therefore, exploring the distribution and provenance of marine sediment types in coastal areas heavily influenced by human activities can provide scientific evidence and references for the current and future ecological management of these sensitive environments. For this reason, we conducted an analysis of the sediment grain size, endmembers, and organic matter content and geochemical elements in the Lu’erhuan River-Malan Island-Sandun Island area in the eastern part of Qinzhou Bay, a region heavily influenced by human activities. The sediment grain size clearly differs throughout the study site and the material provenances and hydrodynamic conditions also vary, likely due to the local environmental conditions and the significant impact that human activities have had on the area. The finest-grained sediment is imported from either inland or coastal areas via rivers and weak tidal currents, the next finest component comes from coastal areas through weak tidal currents, and the moderately coarse component mainly originates from nearby beaches. The two coarsest-grained sediment components are influenced by the combination of human activities, tidal currents and waves and enter the water via erosion. The organic matter provenance resembles that of the sediment components, exhibiting varied characteristics. Due to the combination of natural and human activities in the bay, the organic matter in the upper reaches of the Lu’erhuan River originates from the river and coastal paddy fields, with obvious terrigenous characteristics; the organic matter in northern Malan Island mainly comes from external sources related to oyster farming, while the organic matter in eastern Sandun Island is mainly produced endogenously by marine plankton. Al, Ti, Fe, Mg, K, Ga and other elements indicate that terrestrial sediments are significantly disturbed by human activities. However, Mn reflects the marine distribution of terrestrial sediments from the Lu’erhuan River to Jishuimen. Ca and Sr, which are indicators of marine sediments, are distributed in the eastern offshore area of Sandun Island, which is connected to open waters. Due to the influence of human activities, As and Cd are highly enriched in the study area, while Cu is less affected by human activities.
Structural Effects of Mass Distributions in a Floating Photovoltaic Power Plant
Authors: Li Chun Bao , Choung Joonmo
Published: November 2022

This study deals with a solar photovoltaic demonstration project composed of four types of sub-plants that will be operated in the Saemangeum Seawall coast. The project aimed to investigate the most efficient sub-plant types. Hydrodynamic analyses were undertaken to obtain the loads exerted on the floating photovoltaic power plants on which two kinds of frame structures supported shed- and gable-type photovoltaic panels, producing the four types of sub-plants composed of three floaters. Hydrodynamic interactions between the floaters were considered because floaters were linked with hinge joints. The pressure and acceleration response operator amplitudes were transferred to the finite element analysis model using an in-house code. Because each sub-plant had a different mass and second moments of mass, it was found that huge stresses had been retained in hinge joints. After the masses in the twelve floaters were evenly distributed, the maximum stresses were reduced so that they were less than material yield strengths. There were larger stresses in the POSCO (Pohang Iron and Steel Company) magnesium alloy coating (POSMAC) frames than in the fiber-reinforced plastic (FRP) frames because the POSMAC frame had an open-channel section. It is concluded that weight in each floating unit should be evenly controlled if hinged joints are used to link the floaters.
Numerical Investigation on Behavior of Compressive Piles in Coastal Tidal Flat with Fill
Authors: Shen Kanmin , Wang Kuanjun , Yao Juntian , Yu Jian
Published: November 2022

The control centers of wind power plants are usually located in coastal tidal flat areas. A thick fill should be placed at the original ground level to ensure that the design elevation of the control centers is maintained above the water table. However, the filling would cause a long-term ground settlement and further lead to the development of the negative skin friction (NSF) of the pile foundations for the control centers. The CPTU tests were conducted to calibrate the soil properties, of which the rationalities were verified by comparisons of the pile-bearing capacities between the full-scale axial compressive tests and β-method. The numerical analysis method was then established to investigate the influence of additional ground pressures on the pile axial bearing behavior over time and the influence of NSF caused by consolidation on pile-bearing capacity. Finally, a simple procedure was further employed to investigate the evolution of the long-term pile-bearing behavior.
Selenium Improved Phenylacetic Acid Content in Oilseed Rape and Thus Enhanced the Prevention of Sclerotinia sclerotiorum by Dimethachlon
Authors: Zhang Huan , Cheng Qin , Wang Xu , Jia Wei , Xie Jiatao , Fan Guocheng , Han Chuang , Zhao Xiaohu
Published: November 2022

Sclerotinia sclerotiorum is a broad-spectrum necrotrophic phytopathogen that can infect many plant species worldwide. The application of fungicides is a common measure for controlling Sclerotinia sclerotiorum. Due to the risk of developing resistance to fungicides, it is imperative to find ways to be environmentally friendly and even effective. Using bioactive compounds in plants to reduce the amounts of fungicides has become a clean and sustainable strategy of controlling Sclerotinia sclerotiorum. Our study found that selenium in soil mediated the phenylacetic acid-related metabolic pathway in oilseed rape and reduced the incidence rate of Sclerotinia sclerotiorum. The growth-inhibition rates of Sclerotinia sclerotiorum were observed at 25.82%, 19.67%, and 52.61% for treatments of 0.8 mg·L−1 dimethachlon, 0.1 mg·mL−1 phenylacetic acid, and dimethachlon (0.8 mg·L−1) + phenylacetic acid (0.1 mg·mL−1), respectively. Phenylacetic acid reduced the application amount of dimethachlon and enhanced the inhibition effect for Sclerotinia sclerotiorum. Results also suggested that phenylacetic acid severely damaged the morphological structure, changed the electrical conductivity, and reduced the capacity of acid production and oxalic acid secretion of Sclerotinia sclerotiorum mycelium. Further studies revealed that phenylacetic acid increased the gene-expression level of Ssodc1, Ssodc2, CWDE2 and CWDE10 in mycelium while decreasing the expression level of SsGgt1, and phenylacetic acid + dimethachlon reduced the relative expression level of SsBil. These findings verified that phenylacetic acid could partially replace the amount of dimethachlon, as well as enhance the prevention of Sclerotinia sclerotiorum by dimethachlon, which provides evidence for developing an environment-friendly method for Sclerotinia sclerotiorum control.
Non-Surgical Transversal Dentoalveolar Compensation with Completely Customized Lingual Appliances versus Surgically Assisted Rapid Palatal Expansion in Adults—The Amount of Posterior Crossbite Correction
Authors: Schmid Jonas Q. , Gerberding Elena , Hohoff Ariane , Kleinheinz Johannes , Stamm Thomas , Middelberg Claudius
Published: November 2022

The aim of this study was to compare the crossbite correction of a group (n = 43; f/m 19/24; mean age 27.6 ± 9.5 years) with surgically assisted rapid palatal expansion (SARPE) versus a non-surgical transversal dentoalveolar compensation (DC) group (n = 38; f/m 25/13; mean age 30.4 ± 12.9 years) with completely customized lingual appliances (CCLA). Arch width was measured on digital models at the canines (C), second premolars (P2), first molars (M1) and second molars (M2). Measurements were obtained before treatment (T0) and at the end of lingual treatment (T1) or after orthodontic alignment prior to a second surgical intervention for three-dimensional bite correction. There was no statistically significant difference (p > 0.05) in the amount of total crossbite correction between the SARPE and DC-CCLA group at C, P2, M1 and M2. Maxillary expansion was greater in the SARPE group and mandibular compression was greater in the DC-CCLA group. Crossbite correction in the DC-CCLA group was mainly a combination of maxillary expansion and mandibular compression. Dentoalveolar compensation with CCLAs as a combination of maxillary expansion and mandibular compression seems to be a clinically effective procedure to correct a transverse maxillo-mandibular discrepancy without the need for surgical assistance.
Preliminary Experience of Liquid Biopsy in Lung Cancer Compared to Conventional Assessment: Light and Shadows
Authors: Montella Marco , Ciani Giovanni , Granata Vincenza , Fusco Roberta , Grassi Francesca , Ronchi Andrea , Cozzolino Immacolata , Franco Renato , Zito Marino Federica , Urraro Fabrizio , Monti Riccardo , Sirica Roberto , Savarese Giovanni , Chianese Ugo , Nebbioso Angela , Altucci Lucia , Vietri Maria Teresa , Nardone Valerio , Reginelli Alfonso , Grassi Roberta
Published: November 2022

Purpose: To assess the qualitative relationship between liquid biopsy and conventional tissue biopsy. As a secondary target, we evaluated the relationship between the liquid biopsy results and the T stage, N stage, M stage, and compared to grading. Methods: The Local Ethics Committee of the “Università degli Studi della Campania Luigi Vanvitelli”, with the internal resolution number 24997/2020 of 12.11.2020, approved this spontaneous prospective study. According to the approved protocol, patients with lung cancer who underwent Fine-Needle Aspiration Cytology (FNAC), CT-guided biopsy, and liquid biopsy were enrolled. A Yates chi-square test was employed to analyze differences in percentage values of categorical variables. A p-value < 0.05 was considered statistically significant. Data analysis was performed using the Matlab Statistic Toolbox (The MathWorks, Inc., Natick, MA, USA). Results: When a genetic mutation is present on the pathological examination, this was also detected on the liquid biopsy. ROS1 and PDL1 mutations were found in 2/29 patients, while EGFR Exon 21 was identified in a single patient. At liquid biopsy, 26 mutations were identified in the analyzed samples. The mutations with the highest prevalence rate in the study populations were: ALK (Ile1461Val), found in 28/29 patients (96.6%), EML4 (Lys398Arg), identified in 16/29 (55.2%) patients, ALK (Asp1529Glu), found in 14/29 (48.3%) patients, EGFR (Arg521Lys), found in 12/29 (41.4%) patients, ROS (Lys2228Gln), identified in 11/29 (37.9%) patients, ROS (Arg167Gln) and ROS (Ser2229Cys), identified in 10/29 (34.5%) patients, ALK (Lys1491Arg) and PIK3CA (Ile391Met), identified in 8/29 (27.6%) patients, ROS (Thr145Pro), identified in 6/29 (20.7%) patients, and ROS (Ser1109Leu), identified in 4/29 (13.8%) patients. No statistically significant differences can be observed in the mutation rate between the adenocarcinoma population and the squamous carcinoma population (p > 0.05, Yates chi-square test). Conclusions: We showed that, when a genetic mutation was detected in pathological examination, this was always detected by liquid biopsy, demonstrating a very high concordance rate of genomic testing between tissues and their corresponding mutations obtained by liquid biopsy, without cases of false-negative results. In addition, in our study, liquid biopsy highlighted 26 mutations, with the prevalence of ALK mutation in 96.6% of patients, supporting the idea that this approach could be an effective tool in cases with insufficient tumor tissue specimens or in cases where tissue specimens are not obtainable.
Adverse Effects in Patients with Intellectual and Developmental Disabilities Hospitalized at the University Clinical Hospital
Authors: Alonso-Sardón Montserrat , Sáez-Lorenzo María , Chamorro Antonio Javier , Fernández-Martín Luz Celia , Iglesias-de-Sena Helena , González-Núñez Verónica , Santos-Sánchez José Ángel , Carbonell Cristina , Lorenzo-Gómez María Fernanda , Mirón-Canelo José Antonio
Published: November 2022

(1) Background: Providing the patient with the health care they need in a personalized and appropriate manner and without adverse effects (AEs) is a part of quality of care and patient safety. The aim of this applied research project was the assessment of AEs as a clinical risk in patients with high social vulnerability such as persons with intellectual and developmental disabilities (PwIDD). (2) Methods: A retrospective epidemiological cohort study was performed on exposed and unexposed groups (the control group) in order to estimate the incidence of AEs in PwIDDs and assess their importance for this category of patients. (3) Results: AEs were observed with a frequency of 30.4% (95% CI) in the PwIDD exposed group, with significant differences to the unexposed group (p = 0.009). No differences were observed with regards to gender. Age was as a marker of care risk, with the highest incidence of AEs in the group of 60–69 years. (4) Conclusions: PwIDDs have a high risk of suffering AEs while receiving health care assistance due to their high social and clinical vulnerability. Health care practitioners must therefore be aware of these results and keep these observations in mind in order to carry out personalized, preventive, competent, effective, and safe medical care.
How Can the Risk of Misconduct in Land Expropriation for Tract Development Be Prevented and Mitigated: A Study of “Good Land Governance” Inspection in China
Authors: Li Lingling , He Yansong , Li Changjian
Published: November 2022

In the context of China’s new round of land reform, narrowing the scope of land expropriation, standardising the procedures for land expropriation, and building a unified urban and rural construction land market have become the objectives of land expropriation reform. The new Land Management Law of the People’s Republic of China confirms land expropriation for tract development as a new situation for the land acquisition system. However, in the process of implementing the system, the new land acquisition mode is plagued by the dual dilemma of the falsification of public interests and the lack of a mechanism to realise public interests, which leads to the real risk of misconduct in standards. The concept of the coordination of interests and co-operative governance in “Good Land Governance” is a sure way to overcome this structural risk. The article analyses the causes through the lens of “Good Land Governance” and concludes that the risk of failure of the standards can be addressed through the binary public good remedy: On the one hand, from the perspective of coordination of interests, drawing on the principle of proportionality, the system can be built by improving the way of purposeful examination, so as to achieve the effect of preventing the risk of deflating and generalising public interests in order to achieve the effect of realising and reinforcing public interests. On the other hand, from the perspective of synergistic shared governance and drawing on the logic of land justice, institutional insight can be built by way of establishing a pattern of shared public interest, thus achieving the effect of preventing the risk of public interest erosion in order to achieve the effect of shared public interest replenishment.
Impacts of Urban Spatial Development Patterns on Carbon Emissions: Evidence from Chinese Cities
Authors: Li Xuanting , Wang Xiaohong , Zhang Shaopeng
Published: November 2022

A rational urban spatial development pattern is necessary for China to optimize urban resource allocation and promote low-carbon urban development. Although research on carbon emission reduction has increased, few studies examine the impact of urban spatial development patterns from a spatial–structural perspective. This study uses static and dynamic spatial Durbin models to analyze the dynamic impact of urban spatial development patterns on carbon emissions based on municipal-level statistical data and LandScan high-resolution global population distribution data from 2004 to 2019. The empirical results show that (1) urban spatial development patterns characterized by coefficients of variation have an inverted U-shaped relationship with carbon emissions; (2) direct spatial spillover effects have a long-term U-shaped relationship, while indirect effects have an inverted U-shaped relationship; (3) by analyzing the heterogeneity of city sizes and geographical area, the smaller the city is, the more effectively the compact urban spatial development pattern reduces carbon emissions; and (4) compact urban spatial development patterns in the west suppress carbon emissions compared to the east and central regions. The findings of this paper have policy implications for optimizing spatial development patterns and achieving low-carbon development in Chinese cities.
Social Dimensions of Spatial Justice in the Use of the Public Transport System in Thessaloniki, Greece
Authors: Tzanni Olga , Nikolaou Paraskevas , Giannakopoulou Stella , Arvanitis Apostolos , Basbas Socrates
Published: November 2022

Greek cities hold important urban issues related to social injustice; lack of open spaces, pedestrian facilities, parks and greenery, access to public amenities, etc. In view of these, we investigated residents’ preferences and choices regarding their walking activities in the urban environment of Thessaloniki, Greece. In specific, we applied research with questionnaires to the residents of the municipality of Ampelokipoi-Menemeni. The research applied the Contingent Valuation Method to investigate residents’ willingness to pay (WTP) for the improvement of the existing infrastructure of public space in view of making walking and the use of local metro stations more attractive. A methodological framework was developed, including Explanatory Factor Analysis and Structural Equation Modelling, along with statistical analysis of the results. The lack of pedestrian facilities and the long walking distances are two main reasons discouraging the use of the metro. CVM application showed that almost half of the residents would be willing to contribute an amount of money for the improvement of pedestrian infrastructure. The main reasons for this were to make the use of the metro more attractive, which would also improve their living conditions and would contribute to a better quality of life. Income restrictions proved highly important to a negative WTP.
Climatic and Altitudinal Variation in Physicochemical Properties of Citrus sinensis in India
Authors: Anand Jahan , Rawat Jagmohan Singh , Rawat Vidyawati , Singh Bhupendra , Khanduri Vinod Prasad , Riyal Manoj Kumar , Kumar Prabhat , Pinto Marina M. S. Cabral , Kumar Munesh
Published: November 2022

C. sinensis (L.) Osbeck is grown in large-scale, in-home gardens of traditional agroforestry systems in the Garhwal Himalaya. The present investigation of C. sinensis was conducted on the basis of twenty-six populations in different home gardens located in different geographical variables. The aim was to understand variations in physicochemical properties, viz., fruit weight, fruit length, fruit width, peel thickness, peel weight, juice sacks, juice weight, seeds/fruits, rudimentary seeds/fruits, seed weight, total soluble solids, citric acid, ascorbic acid and sugar content. The results of the study revealed that the physicochemical properties of C. sinensis were significantly varied among the populations. The fruit weight was the most variable among the morphological characteristics, and vitamin C among the nutritive parameters. The average fruit weight varied from 96.64 to 296.86 g/fruit. The other study traits were found to oscillate between 7.73 and 12.76 °Brix (total soluble solids); 3.04 and 5.96% (citric acid); 31.45 and 86.45 mg/g−1 (vitamin C) and 4.65 and 9.27% soluble sugar. Geographical variables (altitude, latitude and longitude, temperature and rainfall) have influenced the physicochemical parameters of C. sinensis significantly. Amongst the physicochemical properties, fruit weight and vitamin C were the most variable parameters and could be used for the improvement of C. sinensis. These variations in the C. sinensis population have enabled great advances in better understanding the variability in the physicochemical properties of fruit and response to biotic and abiotic stresses.
Molecular Breeding and Drought Tolerance in Chickpea
Authors: Asati Ruchi , Tripathi Manoj Kumar , Tiwari Sushma , Yadav Rakesh Kumar , Tripathi Niraj
Published: November 2022

Cicer arietinum L. is the third greatest widely planted imperative pulse crop worldwide, and it belongs to the Leguminosae family. Drought is the utmost common abiotic factor on plants, distressing their water status and limiting their growth and development. Chickpea genotypes have the natural ability to fight drought stress using certain strategies viz., escape, avoidance and tolerance. Assorted breeding methods, including hybridization, mutation, and marker-aided breeding, genome sequencing along with omics approaches, could be used to improve the chickpea germplasm lines(s) against drought stress. Root features, for instance depth and root biomass, have been recognized as the greatest beneficial morphological factors for managing terminal drought tolerance in the chickpea. Marker-aided selection, for example, is a genomics-assisted breeding (GAB) strategy that can considerably increase crop breeding accuracy and competence. These breeding technologies, notably marker-assisted breeding, omics, and plant physiology knowledge, underlined the importance of chickpea breeding and can be used in future crop improvement programmes to generate drought-tolerant cultivars(s).
Automatic Detection of Tuberculosis Using VGG19 with Seagull-Algorithm
Authors: Mohan Ramya , Kadry Seifedine , Rajinikanth Venkatesan , Majumdar Arnab , Thinnukool Orawit
Published: November 2022

Due to various reasons, the incidence rate of communicable diseases in humans is steadily rising, and timely detection and handling will reduce the disease distribution speed. Tuberculosis (TB) is a severe communicable illness caused by the bacterium Mycobacterium-Tuberculosis (M. tuberculosis), which predominantly affects the lungs and causes severe respiratory problems. Due to its significance, several clinical level detections of TB are suggested, including lung diagnosis with chest X-ray images. The proposed work aims to develop an automatic TB detection system to assist the pulmonologist in confirming the severity of the disease, decision-making, and treatment execution. The proposed system employs a pre-trained VGG19 with the following phases: (i) image pre-processing, (ii) mining of deep features, (iii) enhancing the X-ray images with chosen procedures and mining of the handcrafted features, (iv) feature optimization using Seagull-Algorithm and serial concatenation, and (v) binary classification and validation. The classification is executed with 10-fold cross-validation in this work, and the proposed work is investigated using MATLAB® software. The proposed research work was executed using the concatenated deep and handcrafted features, which provided a classification accuracy of 98.6190% with the SVM-Medium Gaussian (SVM-MG) classifier.
Fermented Soybean Meal (FSBM) in African Catfish (Clarias gariepinus) Diets: Effects on Growth Performance, Fish Gut Microbiota Analysis, Blood Haematology, and Liver Morphology
Authors: Zakaria Muhammad Khairulanam , Kari Zulhisyam Abdul , Van Doan Hien , Kabir Muhammad Anamul , Che Harun Hasnita , Mohamad Sukri Suniza Anis , Goh Khang Wen , Wee Wendy , Khoo Martina Irwan , Wei Lee Seong
Published: November 2022

The study revealed the potential of fermented soybean meal (FSBM) as a fish meal (FM) replacement in African catfish (Clarias gariepinus) feed formulation. Five isonitrogenous diets (32% crude protein) were prepared with five different levels of FSBM as FM replacement, namely 0% FSBM (T1), 40% FSBM (T2), 50% FSBM (T3), 60% FSBM (T4), and 70% (T5). The experimental fish was given the formulated diet for eight consecutive weeks. At the end of the feeding trial, the fish were subjected to growth performance, blood parameters, blood chemical, liver histology, and gut microbiota assessment. The study findings demonstrated that the experimental fish that received the T2 diet exhibited significantly higher (p < 0.05) growth performance. Experimental fish that received diet T2 had significantly higher (p < 0.05) white blood cell (WBC) and significantly lower (p < 0.05) in terms of cholesterol (CHOL), albumin (ALB), globulin (GLOB), and total protein (TP). The replacement of FSBM to FM significantly affected liver morphology on the sinusoid, vacuole, nucleus, and erythrocytes. Gut microbiota composition analysis showed a significantly high abundance (p < 0.05) of Akkermansia muciniphila in the experimental fish that received the T2 diet. The gut microbiota indicates that the experimental fish is in a healthy condition. In conclusion, replacing 40% FSBM with FM in aquafeed could enhance C. gariepinus growth performance and health conditions.
Bioactivity Potential of Bioceramic-Based Root Canal Sealers: A Scoping Review
Authors: Estivalet Mauro Schmitz , de Araújo Lucas Peixoto , Immich Felipe , da Silva Adriana Fernandes , Ferreira Nadia de Souza , da Rosa Wellington Luiz de Oliveira , Piva Evandro
Published: November 2022

Introduction: Bioceramic-based root canal sealers are novel materials with a bioactivity potential that stands out compared with conventional root canal sealers. However, the term bioactivity may be overused and is often misunderstood. Hence, the objective of this study was to synthesize and map key concepts related to the bioactivity analysis of bioceramic-based root canal sealers. Methods: The present scoping review is reported in accordance with the PRISMA-ScR Statement and is registered in the Open Science Framework. Two blinded reviewers carried out a comprehensive search in six databases up to January 10th, 2022: MEDLINE, Scopus, Embase, Web of Science, Cochrane Library, and Lilacs/BBO. Eligibility was considered for in vitro and in vivo studies that evaluated the bioactivity potential of bioceramic-based root canal sealers. Results: A total of 53 studies were included in the qualitative synthesis. In vitro bioactivity was evaluated through the mineralization potential, formation of carbonated apatite on the surface, and the gene expression related to proteins involved in the mineralization process. Meanwhile, for in vivo studies, staining techniques associated with immunohistochemical tests were mainly used to detect mineralization on the material–host tissue interface. Conclusions: According to the methodology used, the most prevalent methods to assess bioactivity in acellular form were the immersion of the material in Hank’s balanced salt solution, followed by surface observation with scanning electron microscopy and energy dispersive X-ray. In cell cultures, the chosen method was usually Alizarin Red staining, followed by the evaluation of alkaline phosphatase enzymatic activity and the use of molecular biology tests.
Pharmaco-Toxicological Assessment of the Combined Cytotoxic Effects of Digoxin and Betulinic Acid in Melanoma Cells
Authors: Rednic Robert , Macasoi Ioana , Pinzaru Iulia , Dehelean Cristina Adriana , Tomescu Mirela-Cleopatra , Susan Monica , Feier Horea
Published: November 2022

Betulinic acid, a small molecule from pentacyclic triterpenes class, has been widely studied for its antitumor activity, revealing that it induces the apoptosis of tumor cells in a selective manner. In recent years, digoxin, a cardiac glycoside found particularly in the plant species Digitalis lanata, has drawn interest for its potential antitumor properties. The present study was designed to evaluate the antimelanoma potential of betulinic acid (BA), digoxin (DG), and their association (DG + BA). In vitro assessments were performed 24 h post-treatment on two human melanoma cell lines (SK-Mel-28 and RPMI-7951). In addition, the potential irritant effects of the test samples were evaluated using the chorioallantoic membrane of hen’s eggs. BA and DG exhibit a concentration-dependent cytotoxic activity, with the combination of the two having a more marked effect on the decrease in cell viability (~17% for SK-Mel-28 cells and ~23% for RPMI-7951 cells). Further, morphological changes (rounding of the cells and their separation from the plaque) and alterations in the nucleus and actin fibers (condensation of chromatin and actin fibers, formation of apoptotic bodies) were observed, indicating an apoptotic-like process. Moreover, no irritating effects were observed in ovo. As a result, DG + BA acid may have synergistic potential in the antitumor treatment of melanoma, but future studies are needed in order to clarify the biological mechanisms involved.
Favorable Effects of Virgin Coconut Oil on Neuronal Damage and Mortality after a Stroke Incidence in the Stroke-Prone Spontaneously Hypertensive Rat
Authors: Vitor Rodel Jonathan Santos , Tochinai Ryota , Sekizawa Shin-Ichi , Kuwahara Masayoshi
Published: November 2022

Stroke is consistently one of the top ten causes of morbidity and mortality globally, whose outcomes are quite variable, necessitating case-specific management. Prophylactic diets before the onset of stroke have been implicated to work. In this research, the effects of virgin coconut oil (VCO) on stroke were evaluated using a stroke-prone spontaneously hypertensive rat (SHRSP) model. Eight-week-old SHRSPs were subjected to the repeated oral administration (5 mL/kg/day) of either 1% Tween 80 (group A) or VCO (group B). An early stroke onset was observed due to hypertension that was aggravation by the administration of 1% NaCl in water ad libitum. The following data were collected: the days until stroke occurred, the survival rate until the animal died, and blood pressure (BP) every two weeks using the tail-cuff method. After necropsy, the organs were harvested, and the brain was processed for a routine histopathological analysis. VCO delayed the incidence of it and prolonged their survival. Compared to group A, group B showed a significantly lowered BP by 20 mmHg at four weeks after the start of VCO treatment. Lastly, the brain histopathology showed that the structurally damaged areas were smaller in group B than they were in group A. The VCO could have protective effects on the brain before and even after stroke incidence.