bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of “Wine Country of Origin” on the Perception of Wines by South African and French Wine Consumers: A Cross-Cultural Comparison
Authors: Valentin Dominique , Valente Carlo , Ballester Jordi , Symoneaux Ronan , Smith Ina , Bauer Florian F. , Nieuwoudt Helene
Published: July 2021

Culture is an important factor that influences how marketing interacts with food choice. This study aims at exploring the effect of consumers’ Country of Origin (COO) on wine representations and perception using Chenin blanc as a model. The first objective was to evaluate the role of origin in the construction of the representation. We used the theoretical framework of social representation to compare South African (SA) and French consumers’ representations via a word association task. The results indicated that SA representations are dominated by consumers’ experience of the wine (sensory and emotional dimensions), whereas French representations are dominated by the wine itself, in particular its origin and mode of consumption. The second objective was to evaluate the effect of origin on wine categorization in two conditions: with and without information concerning the two geographical origins of the samples. Results showed that providing information on the origin of the wines affected French participants more than SA participants. In both conditions, the groups of wines formed in the sorting tasks by SA participants were based on sensory descriptors and appeared not to be impacted by the information on origin. In contrast, providing information on the origin of the wines to French participants led to an increased use of the words “Loire”, “South Africa” and “familiar” suggesting a different sorting strategy more deliberately based on the origin of the wines. Our findings have important implications for the marketing and export activities within the wine industry.
Mechanical Property of Polypropylene Gels Associated with That of Molten Polypropylenes
Authors: Ouchi Tetsu , Yamazaki Misuzu , Maeda Tomoki , Hotta Atsushi
Published: July 2021

This study aims to understand the fundamental mechanical relationship between polypropylene (PP)-gels and solid PPs without solvent through mechanical and thermal analyses, by which the mechanical similarities between molten PPs and PP gels were found, leading to the reliable estimate of the mechanical properties of semi-crystalline gels. The gelation of syndiotactic and isotactic polypropylenes (sPP and iPP) was found when PPs were dissolved in 1,2,3,4-tetrahydronaphthalene (tetralin). Interestingly, it was found that the storage modulus of sPP-gel became higher than that of iPP-gel at low PP concentration (<~40 wt%). The result was distinctly different from the result of neat solid PPs (without solvent), where the modulus of solid sPP is generally significantly lower than that of solid iPP. Such inversion behavior in the mechanical property of semi-crystalline gels had not been reported and discussed before. By further investigation of the storage moduli of neat sPP and iPP, it was found that the storage modulus of sPP became higher than that of iPP above the melting points of PP, which was similar to the behavior of the storage moduli observed in the diluted PP-gels. Such similarity between PP-gels and PP melts was also observed within iPP samples with different molecular weights.
Additive Fabrication and Characterization of Biomimetic Composite Bone Scaffolds with High Hydroxyapatite Content
Authors: Lee Hoyeol , Yoo Jin Myoung , Nam Seung Yun
Published: July 2021

With the increased incidence of bone defects following trauma or diseases in recent years, three-dimensional porous scaffolds fabricated using bioprinting technologies have been widely explored as effective alternatives to conventional bone grafts, which provide cell-friendly microenvironments promoting bone repair and regeneration. However, the limited use of biomaterials poses a significant challenge to the robust and accurate fabrication of bioprinted bone scaffolds that enable effective regeneration of the target tissues. Although bioceramic/polymer composites can provide tunable biomimetic conditions, their effects on the bioprinting process are unclear. Thus, in this study, we fabricated hydroxyapatite (HA)/gelatin composite scaffolds containing large weight fractions of HA using extrusion-based bioprinting, with the aim to provide an adequate biomimetic environment for bone tissue regeneration with compositional and mechanical similarity to the natural bone matrix. The overall features of the bioprinted HA/gelatin composite scaffolds, including rheological, morphological, physicochemical, mechanical, and biological properties, were quantitatively assessed to determine the optimal conditions for both fabrication and therapeutic efficiency. The present results show that the bioprinted bioceramic/hydrogel scaffolds possess excellent shape fidelity; mechanical strength comparable to that of native bone; and enhanced bioactivity in terms of cell proliferation, attachment, and osteogenic differentiation. This study provides a suitable alternative direction for the fabrication of bioceramic/hydrogel-based scaffolds for bone repair based on bioprinting.
Adolescent Obesity Modeling: A Framework of Socio-Economic Analysis on Public Health
Authors: Salarzadeh Jenatabadi Hashem , Shamsi Nurulaini Abu , Ng Boon-Kwee , Abdullah Nor Aishah , Mentri Khairul Anam Che
Published: July 2021

Bayesian Structural Equation Modeling (SEM-Bayesian) was applied across different research areas to model the correlation between manifest and latent variables. The primary purpose of this study is to introduce a new framework of complexity to adolescent obesity modeling based on adolescent lifestyle through the application of SEM-Bayesian. The introduced model was designed based on the relationships among several factors: household socioeconomic status, healthy food intake, unhealthy food intake, lifestyle, body mass index (BMI) and body fat. One of the main contributions of this study is from considering both BMI and body fat as dependent variables. To demonstrate the reliability of the model, especially in terms of its fitting and accuracy, real-time data were extracted and analyzed across 881 adolescents from secondary schools in Tehran, Iran. The output of this study may be helpful for researchers who are interested in adolescent obesity modeling based on the lifestyle and household socioeconomic status of adolescents.
Knowledge Level, Motivators and Barriers of Blood Donation among Students at Qatar University
Authors: Ibrahim Amal Abdulsalam , Koç Muammer , Abdallah Atiyeh M.
Published: July 2021

In Qatar, one out of every ten patients admitted to the hospitals is in urgent need of a blood transfusion or blood products. The aims of this study are as follows: (1) to assess the level of awareness and knowledge about blood donation and (2) to identify the factors that contribute to the willingness to donate blood among young adults. A cross-sectional survey using a constructed questionnaire was conducted among students at Qatar University. A total of 590 responses were collected, out of which 423 were suitable for analysis. Only 72 out of 472 (15%) participants were blood donors. The chi-square test and t-test were then used to study the association of blood donation status with different factors. Significant values were considered to be p ≤ 0.5. Gender and age were found to be significantly associated with blood donation status, with a higher frequency of donation among males and adults above the age of 24 years old. On the other hand, the total knowledge score was found to not be significantly associated with blood donation status with a mean score of 60.5% for both groups (blood donors, non-blood donors). The most common motivators that encouraged blood donors were donating to help people, followed by having a blood mobile unit come to your place, whereas the most common barriers reported by non-blood donors were failing to meet the requirements, followed by “never having been asked to give blood”. This is the first study in Qatar to assess blood donation status. It provides insights that would help in developing effective strategies for the recruitment and retention of young adult blood donors in Qatar and countries with similar cultures. Raising awareness about blood donation, along with providing more mobile blood donation units at public places, will aid in increasing the frequency of blood donation among young adults.
Influences of Kinesio Taping with Therapeutic Exercise in Patients with Low Back Pain
Authors: Jung Kyoung-sim , Jung Jin-hwa , In Tae-sung , Cho Hwi-young
Published: July 2021

The aim of this study was to evaluate the effect of core stability exercise combined with Kinesio taping on pain, endurance, and balance in patients with lower back pain (LBP). 46 patients with LBP were recruited and randomly allocated into the core stability exercise with taping (CSET) group and the core stability exercise (CSE) group. All participants performed core stability exercises for 40 min/day, 5 times/week for 8 weeks, and additional Kinesio taping was applied to the lower backs in the CSET group. The primary outcome measure was the pain intensity using the visual analog scale, and secondary outcome measures were trunk endurance and balance using the Biering-Sorensen test and force plate, respectively. After the intervention, the CSET group showed significant improvements in pain and postural balance compared to the CSE group (p < 0.05). However, there was no significant difference in trunk endurance between two groups (p > 0.05). This study found that core stability exercise was effective in reducing pain and enhancing balance in patients with LBP, and demonstrated that the application of additional Kinesio taping further increased these effects. Therefore, we recommend that core exercise combined with Kinesio taping may be used to improve the pain and postural balance of patients with LBP in clinics.
Hydrological Connectivity in a Permafrost Tundra Landscape near Vorkuta, North-European Arctic Russia
Authors: Tananaev Nikita , Isaev Vladislav , Sergeev Dmitry , Kotov Pavel , Komarov Oleg
Published: July 2021

Hydrochemical and geophysical data collected during a hydrological survey in September 2017, reveal patterns of small-scale hydrological connectivity in a small water track catchment in the north-European Arctic. The stable isotopic composition of water in different compartments was used as a tracer of hydrological processes and connectivity at the water track catchment scale. Elevated tundra patches underlain by sandy loams were disconnected from the stream and stored precipitation water from previous months in saturated soil horizons with low hydraulic conductivity. At the catchment surface and in the water track thalweg, some circular hollows, from 0.2 to 0.4 m in diameter, acted as evaporative basins with low deuterium excess (d-excess) values, from 2‰ to 4‰. Observed evaporative loss suggests that these hollows were disconnected from the surface and shallow subsurface runoff. Other hollows were connected to shallow subsurface runoff, yielding d-excess values between 12‰ and 14‰, close to summer precipitation. ‘Connected’ hollows yielded a 50% higher dissolved organic carbon (DOC) content, 17.5 ± 5.3 mg/L, than the ‘disconnected’ hollows, 11.8 ± 1.7 mg/L. Permafrost distribution across the landscape is continuous but highly variable. Open taliks exist under fens and hummocky depressions, as revealed by electric resistivity tomography surveys. Isotopic evidence supports upward subpermafrost groundwater migration through open taliks under water tracks and fens/bogs/depressions and its supply to streams via shallow subsurface compartment. Temporal variability of isotopic composition and DOC in water track and a major river system, the Vorkuta River, evidence the widespread occurrence of the described processes in the large river basin. Water tracks effectively drain the tundra terrain and maintain xeric vegetation over the elevated intertrack tundra patches.
Evaluation of Wild Flora Surrounding Blueberry Fields as Viable Hosts of Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) in Georgia
Authors: Grant Joshua A. , Sial Ashfaq A.
Published: July 2021

Drosophila suzukii, an economically important pest of small and thin-skinned fruits, has caused annual crop losses up to 20% in the state of Georgia’s multimillion-dollar blueberry industry. The known host range of D. suzukii is large, yet the breadth of uncultivated and wild plants that can serve as alternative hosts in the southeastern United States is still not fully understood. Establishing comprehensive lists of non-crop D. suzukii hosts in woodlands near blueberry production will assist in the creation of more sustainable integrated pest management (IPM) strategies. Objectives of this study were to determine viability of wild fruiting plant species to this pest based on survivorship to adulthood and assess D. suzukii short-range preference between cultivated blueberries and wild fruit. Laboratory choice and no-choice assays were performed to determine if D. suzukii could complete its development on wild fruits sampled from the field. Results from our no-choice assays indicated that multiple species of wild fruits surveyed in Georgia were viable D. suzukii hosts including blackberry species, deerberry, hillside blueberry, common pokeweed, beautyberry, elderberry, evergreen blueberry, and large gallberry. Yet, none of these hosts were preferred by adult female D. suzukii as ovipositional substrates when compared to cultivated blueberries. However, these uncultivated species have the potential to sustain D. suzukii populations pre- and post-harvest season. This information can help farmers do more targeted management of these viable alternative hosts from wooded areas surrounding blueberry fields in order to minimize D. suzukii populations.
Emotions Evoked by Geometric Patterns
Authors: Trautmann Laura
Published: July 2021

Many studies have shown that the appearance of products has a great impact on consumers. In addition to functionality, aesthetics is of paramount importance in many branches of product design. Visual appearance includes, among other things, the colour, shape, and pattern of the product. The effect of shapes and colours is often studied, but not much data are available on the emotional impact of patterns. This study specifically examines the impact of geometric patterns, which may be of help in the work of product designers. Interviews and questionnaires based on a newly introduced method revealed, for instance, that the basic geometry that makes up geometric patterns and the way the pattern is arranged fundamentally influence the effect of the pattern on consumers.
Effect of Deep versus Moderate Neuromuscular Blockade on Quantitatively Assessed Postoperative Atelectasis Using Computed Tomography in Thoracic Surgery; a Randomized Double-Blind Controlled Trial
Authors: Lee Bong-Jae , Lee Han Na , Chung Jun-Young , Kim Daehyun , Kim Jung Im , Seo Hyungseok
Published: July 2021

Background: postoperative atelectasis is a significant clinical problem during thoracic surgery with one-lung ventilation. Intraoperative deep neuromuscular blockade can improve surgical conditions, but an increased risk of residual paralysis may aggravate postoperative atelectasis. Every patient was verified to have full reversal before extubation. We compared the effect of deep versus moderate neuromuscular blockade on postoperative atelectasis quantitatively using chest computed tomography. Methods: patients undergoing thoracic surgery were randomly allocated to two groups: moderate neuromuscular blockade during surgery (group M) and deep neuromuscular blockade during surgery (group D). The primary outcome was the proportion and the volume of postoperative atelectasis measured by chest computed tomography on postoperative day 2. The mean values of the repeatedly measured intraoperative dynamic lung compliance during surgery were also compared. Result: the proportion of postoperative atelectasis did not differ between the groups (1.32 [0.47–3.20]% in group M and 1.41 [0.24–3.07]% in group D, p = 0.690). The actual atelectasis volume was 38.2 (12.8–61.4) mL in group M and 31.9 (7.84–75.0) mL in group D (p = 0.954). Some factors described in the lung protective ventilation were not taken into account and might explain the atelectasis in both groups. The mean lung compliance during one-lung ventilation was higher in group D (26.6% in group D vs. 24.1% in group M, p = 0.026). Conclusions: intraoperative deep neuromuscular blockade did not affect postoperative atelectasis when compared with moderate neuromuscular blockade if full reversal was verified.
Fully Automated Colorimetric Analysis of the Optic Nerve Aided by Deep Learning and Its Association with Perimetry and OCT for the Study of Glaucoma
Authors: Gonzalez-Hernandez Marta , Gonzalez-Hernandez Daniel , Perez-Barbudo Daniel , Rodriguez-Esteve Paloma , Betancor-Caro Nisamar , Gonzalez de la Rosa Manuel
Published: July 2021

Background: Laguna-ONhE is an application for the colorimetric analysis of optic nerve images, which topographically assesses the cup and the presence of haemoglobin. Its latest version has been fully automated with five deep learning models. In this paper, perimetry in combination with Laguna-ONhE or Cirrus-OCT was evaluated. Methods: The morphology and perfusion estimated by Laguna ONhE were compiled into a “Globin Distribution Function” (GDF). Visual field irregularity was measured with the usual pattern standard deviation (PSD) and the threshold coefficient of variation (TCV), which analyses its harmony without taking into account age-corrected values. In total, 477 normal eyes, 235 confirmed, and 98 suspected glaucoma cases were examined with Cirrus-OCT and different fundus cameras and perimeters. Results: The best Receiver Operating Characteristic (ROC) analysis results for confirmed and suspected glaucoma were obtained with the combination of GDF and TCV (AUC: 0.995 and 0.935, respectively. Sensitivities: 94.5% and 45.9%, respectively, for 99% specificity). The best combination of OCT and perimetry was obtained with the vertical cup/disc ratio and PSD (AUC: 0.988 and 0.847, respectively. Sensitivities: 84.7% and 18.4%, respectively, for 99% specificity). Conclusion: Using Laguna ONhE, morphology, perfusion, and function can be mutually enhanced with the methods described for the purpose of glaucoma assessment, providing early sensitivity.
An Early Predictive Scoring Model for In-Hospital Cardiac Arrest of Emergent Hemodialysis Patients
Authors: Chen Shih-Hao , Cheng Ya-Yun , Lin Chih-Hao
Published: July 2021

Background: Patients undergoing hemodialysis are prone to cardiac arrests. Methods: This study aimed to develop a risk score to predict in-hospital cardiac arrest (IHCA) in emergency department (ED) patients undergoing emergency hemodialysis. Patients were included if they received urgent hemodialysis within 24 h after ED arrival. The primary outcome was IHCA within three days. Predictors included three domains: comorbidity, triage information (vital signs), and initial biochemical results. The final model was generated from data collected between 2015 and 2018 and validated using data from 2019. Results: A total of 257 patients, including 52 with IHCA, were analyzed. Statistical analysis selected significant variables with higher sensitivity cutoff, and scores were assigned based on relative beta coefficient ratio: K > 5.5 mmol/L (score 1), pH < 7.35 (score 1), oxygen saturation < 85% (score 1), and mean arterial pressure < 80 mmHg (score 2). The final scoring system had an area under the curve of 0.78 (p < 0.001) in the primary group and 0.75 (p = 0.023) in the validation group. The high-risk group (defined as sum scores ≥ 3) had an IHCA risk of 47.2% and 41.7%, while the low-risk group (sum scores < 3) had 18.3% and 7%, in the primary and validation databases, respectively. Conclusions: This predictive score model for IHCA in emergent hemodialysis patients could help healthcare providers to take necessary precautions and allocate resources.
Effects of Childhood Multidisciplinary Care and Growth Hormone Treatment on Health Problems in Adults with Prader-Willi Syndrome
Authors: Pellikaan Karlijn , Rosenberg Anna G. W. , Davidse Kirsten , Kattentidt-Mouravieva Anja A. , Kersseboom Rogier , Bos-Roubos Anja G. , Grootjen Lionne N. , Damen Layla , van den Berg Sjoerd A. A. , van der Lely Aart J. , Hokken-Koelega Anita C. S. , de Graaff Laura C. G.
Published: July 2021

Prader-Willi syndrome (PWS) is a complex hypothalamic disorder. Features of PWS include hyperphagia, hypotonia, intellectual disability, and pituitary hormone deficiencies. The combination of growth hormone treatment and multidisciplinary care (GHMDc) has greatly improved the health of children with PWS. Little is known about the effects of childhood GHMDc on health outcomes in adulthood. We retrospectively collected clinical data of 109 adults with PWS. Thirty-nine had received GHMDc during childhood and adolescence (GHMDc+ group) and sixty-three had never received growth hormone treatment (GHt) nor multidisciplinary care (GHMDc− group). Our systematic screening revealed fewer undetected health problems in the GHMDc+ group (10%) than in the GHMDc− group (84%). All health problems revealed in the GHMDc+ group had developed between the last visit to the paediatric and the first visit to the adult clinic and/or did not require treatment. Mean BMI and the prevalence of diabetes mellitus type 2 were significantly lower in the GHMDc+ group compared to the GHMDc− group. As all patients who received GHt were treated in a multidisciplinary setting, it is unknown which effects are the result of GHt and which are the result of multidisciplinary care. However, our data clearly show that the combination of both has beneficial effects. Therefore, we recommend continuing GHMDc after patients with PWS have reached adult age.
Application of Three-Dimensional Computed Tomography Improved the Interrater Reliability of the AO/OTA Classification Decision in a Patellar Fracture
Authors: Byun Seong-Eun , Shon Oog-Jin , Sim Jae-Ang , Joo Yong-Bum , Kim Ji-Wan , Na Young-Gon , Choi Wonchul
Published: July 2021

We investigated whether interrater reliabilities of the AO/OTA classification of patellar fracture change with the imaging modalities applied, including plain radiography and two- and three-dimensional (2-D and 3-D) computed tomography (CT). Seven orthopedic specialists and four orthopedic residents completed a survey of 50 patellar fractures to classify the fractures according to the AO/OTA classification for patellar fractures. Initially, the survey was conducted using plain radiography only, then with 2-D CT introduced three weeks later and 3-D CT introduced six weeks later. Fleiss’ Kappa coefficients were calculated to determine interrater reliability. The overall interrater reliability of the AO/OTA classifications was 0.40 (95% CI, 0.38–0.42) with plain radiography only and 0.43 (95% CI, 0.41–0.45) with the addition of 2-D CT. With the addition of 3-D CT, the reliability was significantly improved to 0.54 (95% CI, 0.52–0.56). In specialists, interrater reliability of the classifications was moderate with all three imaging modalities. With the use of 3-D CT, interrater reliability of the classification was 0.53 (95% CI, 0.50–0.56), which was significantly higher than that with the use of 2-D CT (κ = 0.45; 95% CI, 0.42–0.48). In residents, interrater reliability of the classification was 0.30 (95% CI, 0.24–0.36) with plain radiography. The reliability improved to 0.49 (95% CI, 0.43–0.56) with the addition of 2-D CT, which was significantly higher than that with plain radiography only. The use of 3-D CT imaging improved interrater reliability of the classification. Therefore, surgeons, especially residents, may benefit from using 3-D CT imaging for classifying and planning the treatment of patellar fractures.
Effect of Filler Morphology on the Electrical and Thermal Conductivity of PP/Carbon-Based Nanocomposites
Authors: Zaccone Marta , Frache Alberto , Torre Luigi , Armentano Ilaria , Monti Marco
Published: July 2021

In this paper, we studied the effect of different carbon-based nanostructures on the electrical and mechanical properties of polypropylene (PP) nanocomposites. Multi-walled carbon nanotubes (MWCNT), expanded graphite (EG), and two different carbon black nanoparticles (CB) have been dispersed at several weight contents in the polymer matrix through a melt extrusion process. The produced nanocomposites have been used to obtain samples for the characterization by injection molding. The dispersion of the nanoparticles in the matrix has been evaluated by scanning electron microscopy (SEM) analysis. The electrical characterization has been performed both in DC and in AC configuration. The mechanical properties have been evaluated with both tensile test and impact strength (Izod). The thermal conductivity has been also evaluated. As a result, MWCNTs are the nanoadditive with the lowest electrical percolation threshold. This allows MWCNT nanocomposite to drastically change the electrical behavior without a significant embrittlement observed with the other nanoadditives. However, CB with the lowest surface area allows the highest conductivity, even though at a high particle content. EG has a limited effect on electrical properties, but it is the only one with a significant effect on thermal conductivity.
Influence of Warm-Up Music Preference on Anaerobic Exercise Performance in Division I NCAA Female Athletes
Authors: Meglic Corinne E. , Orman Caroline M. , Rogers Rebecca R. , Williams Tyler D. , Ballmann Christopher G.
Published: July 2021

The purpose of this study was to investigate the effects of listening to preferred versus non-preferred warm-up music on anaerobic sprint performance in Division I NCAA female athletes. Female collegiate athletes (n = 14) were recruited for this study. In a counterbalanced, crossover study design, participants completed two separate visits, each with a different warm-up music condition: preferred (Pref) or non-preferred (Non-pref). During each visit, participants completed a 3 min standardized cycling warm-up at 50 Watts while listening to Pref or Non-pref music. Following this, participants completed 3 × 15 s Wingate Anaerobic Tests (WAnTs) with a 2 min active recovery period in between tests. Motivation to exercise was measured immediately following the warm-up (WU), WAnT1, WAnT2, and WAnT3. The rate of perceived exertion (RPE) was also measured after each WAnT. Each visit was separated by a minimal recovery period of 48 h. Mean power, total work, RPE, and motivation were analyzed. Mean power (p = 0.044; d = 0.91) and total work (p = 0.045; d = 0.78) were significantly higher during the Pref music condition versus Non-pref. RPE remained unchanged regardless of condition (p = 0.536; d = 0.01). Motivation was significantly higher with Pref warm-up music compared to Non-pref (p < 0.001; d = 1.55). These results show that listening to Pref warm-up music has an ergogenic benefit during repeated sprints in comparison to Non-pref music and improves motivation to exercise. Listening to warm-up music prior to high-intensity repeated exercise may aid in optimizing performance and training in collegiate athletes.
Relationship between Three-Dimensional Radiation Stress and Vortex-Force Representations
Authors: Nguyen Duoc Tan , Reniers A.J.H.M , Roelvink Dano
Published: July 2021

In numerical ocean models, the effect of waves on currents is usually expressed by either vortex-force or radiation stress representations. In this paper, the differences and similarities between those two representations are investigated in detail in conditions of both conservative and nonconservative waves. In addition, comparisons between different sets of equations of mean motion that apply different representations of wave-induced forcing terms are included. The comparisons are useful for selecting a suitable numerical ocean model to simulate the mean current in conditions of waves combined with currents.
A Novel Core Effector Vp1 Promotes Fungal Colonization and Virulence of Ustilago maydis
Authors: Hoang Cuong V. , Bhaskar Chibbhi K. , Ma Lay-Sun
Published: July 2021

The biotrophic fungus Ustilago maydis secretes a plethora of uncharacterized effector proteins and causes smut disease in maize. Among the effector genes that are up-regulated during the biotrophic growth in maize, we identified vp1 (virulence promoting 1), which has an expression that was up-regulated and maintained at a high level throughout the life cycle of the fungus. We characterized Vp1 by applying in silico analysis, reverse genetics, phenotypic assessment, microscopy, and protein localization and provided a fundamental understanding of the Vp1 protein in U. maydis. The reduction in fungal virulence and colonization in the vp1 mutant suggests the virulence-promoting function of Vp1. The deletion studies on the NLS (nuclear localization signal) sequence and the protein localization study revealed that the C-terminus of Vp1 is processed after secretion in plant apoplast and could localize to the plant nucleus. The Ustilago hordei ortholog UhVp1 lacks NLS localized in the plant cytoplasm, suggesting that the orthologs might have a distinct subcellular localization. Further complementation studies of the Vp1 orthologs in related smut fungi revealed that none of them could complement the virulence function of U. maydis Vp1, suggesting that UmVp1 could acquire a specialized function via sequence divergence.
Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments
Authors: De Padua Jewel C. , dela Cruz Thomas Edison E.
Published: July 2021

Nickel contamination is a serious environmental issue that requires immediate action. In this study, 23 strains of Trichoderma were isolated from terrestrial and marine environments and identified using a polyphasic approach of morphological characterization and ITS gene sequence analysis. The Trichoderma strains were tested for their tolerance and biosorption of nickel. Our results showed the growth of all Trichoderma strains on Trichoderma Selective Medium (TSM) with 50–1200-ppm nickel, indicating their tolerance of this heavy metal even at a relatively high concentration. Six Trichoderma strains (three isolated from terrestrial substrates and three from marine substates) had the highest radial growth on TSM with 50-ppm Ni. Among these fungal isolates, Trichoderma asperellum (S03) isolated from soil exhibited the best growth after 2 days of incubation. For the biosorption of nickel, the accumulation or uptake efficiency by the six selected Trichoderma was determined in Potato Dextrose Broth (PDB) supplemented with 50-ppm Ni using a Flame Atomic Absorption Spectrophotometer (AAS). The percent uptake efficiency of the three strains of T. asperellum (S03, S08, and LL14) was computed to be up to 66%, while Trichoderma virens (SG18 and SF22) and Trichoderma inhamatum (MW25) achieved up to 68% uptake efficiency. Observation of the Trichoderma strains with Scanning Electron Microscopy (SEM) before and after the absorption of nickel showed very minimal damage on the hyphal and conidial surface morphology, but changes in the colonial characteristics were observed. Our study highlighted the potential of terrestrial and marine strains of Trichoderma for the bioremediation of nickel pollution.
Personalized Orthodontics: From the Sagittal Position of Lower Incisors to the Facial Profile Esthetics
Authors: Derwich Marcin , Minch Liwia , Mitus-Kenig Maria , Zoltowska Agata , Pawlowska Elzbieta
Published: July 2021

Background: One of the goals of orthodontic treatment is to obtain maximum facial harmony. The sagittal position of the lower incisors plays a significant role in the planning of orthodontic treatment. The aim of the study was to evaluate the relationship between the sagittal position of lower incisors and facial profile esthetics with reference to the skeletal vertical dimension. Methods: There were 200 patients included in the study. Patients were allocated into three groups, depending on the vertical growth pattern: normal-angle, low-angle, and high-angle cases. Tweed–Merrifield cephalometric analysis was used to assess the sagittal and vertical position of the mandible, as well as to assess the sagittal position of the lower incisors. Results: Z-angle and Frankfort mandibular incisor plane angle (FMIA) decreased significantly (p < 0.001) with the increase of the skeletal vertical dimension. Incisor mandibular plane angle (IMPA) was significantly higher (p < 0.001) in low-angle patients compared to the high-angle ones. Z-angle appeared to be the most accurate parameter (area under curve, AUC = 0.957) describing patients with a convex profile. The cutoff value of Z-angle 68.0° was characterized by the sensitivity of 94.1% and the specificity of 84.3%. Conclusions: The sagittal position of the lower incisors significantly affects the facial profile convexity. The Z-angle is the parameter which most accurately describes the patients with a convex profile.
Treatment-Free Remission—A New Aim in the Treatment of Chronic Myeloid Leukemia
Authors: Kwaśnik Paulina , Giannopoulos Krzysztof
Published: July 2021

Tyrosine kinases inhibitors (TKIs) revolutionized chronic myeloid leukemia (CML) treatment for many years, prolonging patients’ life expectancy to be comparable to age-matched healthy individuals. According to the latest the European LeukemiaNet (ELN) recommendations, CML treatment aims to achieve long-term remission without treatment (TFR), which is feasible in more than 40% of patients. Nearly all molecular relapses occur during the first 6 months after TKI withdrawal and do not progress to clinical relapse. The mechanisms that are responsible for CML relapses remain unexplained. It is suggested that maintaining TFR is not directly related to the total disposing of the gene transcript BCR-ABL1, but it might be a result of the restoration of the immune surveillance in CML. The importance of the involvement of immunocompetent cells in the period of TKI withdrawal is also emphasized by the presence of specific symptoms in some patients with “withdrawal syndrome”. The goal of this review is to analyze data from studies regarding TFRs in order to characterize the elements of the immune system of patients that might prevent CML molecular relapse. The role of modern droplet digital polymerase chain reaction (ddPCR) and next-generation sequencing (NGS) in better identification of low levels of BCR-ABL1 transcripts was also taken into consideration for refining the eligibility criteria to stop TKI therapy.
The Old Boys Club in New Zealand Listed Companies
Authors: Chen Chen , Ding David K. , Wilson William R.
Published: July 2021

The board of directors plays an important role in implementing corporate governance in the firm, as directors have a fiduciary duty to the firm’s shareholders. The effectiveness of directors is a key determinant of corporate value and they need to bring a range of skills and experience to the boardroom. This skill and experience cannot be developed solely within the firm, and most boards incorporate non-executive directors who are or have been directors of other firms. Current research on the benefits of interlocking directorships is mixed between the claim that they bring outside feedback to the table and open decision makers’ minds, and those who think outside directors are a waste of money and can reduce company performance. This paper investigates the extent of interlocking directorship in New Zealand and how it affects corporate performance. Our findings of largely no significant impact on firm performance are consistent with the management control theory of director interlocks; the exceptions support the class hegemony theory that links interlocking directorship with a negative firm performance.
Global Prevalence of Osteoporosis in Chronic Kidney Disease: Protocol for a Systematic Review
Authors: Duarte Marvery P. , Ribeiro Heitor S. , Neri Silvia G. R. , Almeida Lucas S. , Viana João L. , Lima Ricardo M.
Published: July 2021

Abnormalities in mineral metabolism, soft tissue calcifications, and bone health are common in people with chronic kidney disease (CKD). In this scenario, osteoporosis is a highly prevalent skeletal disorder characterized by reduced bone strength predisposing patients to adverse health outcomes. We will summarize the evidence of the prevalence of osteoporosis in adults with CKD. Methods: We will perform a comprehensive literature search using MEDLINE, EMBASE, Web of Science, CINAHL, and LILACS databases, without date or language restrictions from inception until January 2021. We will include cross-sectional, case–control, or cohort studies that report prevalence data of osteoporosis in adults aged ≥18 years with CKD in stages 3a–5, including those receiving kidney replacement therapies. We will exclude conference abstracts and experimental studies. The primary outcome will be the prevalence of osteoporosis according to the World Health Organization criteria (T-score ≤ −2.5). Two independent reviewers will screen title and abstract, full-text review, critical appraisal of the quality of studies, risk of bias, heterogeneity, and data extraction. The quality of the included studies will be assessed with the Joanna Briggs Institute (JBI) appraisal checklist. The overall prevalence of the studies will be synthesized using random-effects meta-analysis. This systematic review will be reported according to the guidelines for Meta-Analyses and Systematic Reviews of Observational Studies (MOOSE) and the JBI methodological guidance for systematic reviews of observational epidemiological studies. The qualitative and quantitative results will be synthesized and presented in tables, figures or graphs.
Red-Listed Ecosystem Status of Interior Wetbelt and Inland Temperate Rainforest of British Columbia, Canada
Authors: DellaSala Dominick A. , Strittholt James R. , Degagne Rebecca , Mackey Brendan , Werner Jeffery R. , Connolly Michelle , Coxson Darwyn , Couturier Andrew , Keith Heather
Published: July 2021

The Interior Wetbelt (IWB) of British Columbia, which includes the globally rare Inland Temperate Rainforest (ITR), contains primary forests poorly attributed and neglected in conservation planning. We evaluated the IWB and ITR using four IUCN Red List of Ecosystems Criteria: geographic distribution, environmental degradation (abiotic and biotic factors), and likelihood of ecosystem collapse. Clearcut logging (3.2M ha) represented 57% of all anthropogenic disturbances, reducing potential primary forest by 2.7 million ha (28%) for the IWB and 524,003 ha (39%) for the ITR. Decadal logging rates nearly doubled from 5.3% to 10.2% from 1970s–2000s. Core areas (buffered by 100-m from roads and developments) declined by 70% to 95% for the IWB and ITR, respectively. Vulnerable was assigned to karst, the only abiotic factor assessed, because it was associated with rare plants. For biotic factors, Old-Growth Birds were Vulnerable, Southern Woodland Caribou (Rangifer tarandus caribou) habitat and Sensitive Fish were Endangered, and Old-Growth Lichens habitat was Critical. Overall, the IWB was ranked as Endangered and the ITR as Critical with core area collapse possible within 9 to 18 years for the ITR, considered one of the world’s most imperiled temperate rainforests.
Alteration of Genomic Imprinting after Assisted Reproductive Technologies and Long-Term Health
Authors: Ochoa Eguzkine
Published: July 2021

Assisted reproductive technologies (ART) are the treatment of choice for some infertile couples and even though these procedures are generally considered safe, children conceived by ART have shown higher reported risks of some perinatal and postnatal complications such as low birth weight, preterm birth, and childhood cancer. In addition, the frequency of some congenital imprinting disorders, like Beckwith–Wiedemann Syndrome and Silver–Russell Syndrome, is higher than expected in the general population after ART. Experimental evidence from animal studies suggests that ART can induce stress in the embryo and influence gene expression and DNA methylation. Human epigenome studies have generally revealed an enrichment of alterations in imprinted regions in children conceived by ART, but no global methylation alterations. ART procedures occur simultaneously with the establishment and maintenance of imprinting during embryonic development, so this may underlie the apparent sensitivity of imprinted regions to ART. The impact in adulthood of imprinting alterations that occurred during early embryonic development is still unclear, but some experimental evidence in mice showed higher risk to obesity and cardiovascular disease after the restriction of some imprinted genes in early embryonic development. This supports the hypothesis that imprinting alterations in early development might induce epigenetic programming of metabolism and affect long-term health. Given the growing use of ART, it is important to determine the impact of ART in genomic imprinting and long-term health.
The Baseline Structure of the Enteric Nervous System and Its Role in Parkinson’s Disease
Authors: Natale Gianfranco , Ryskalin Larisa , Morucci Gabriele , Lazzeri Gloria , Frati Alessandro , Fornai Francesco
Published: July 2021

The gastrointestinal (GI) tract is provided with a peculiar nervous network, known as the enteric nervous system (ENS), which is dedicated to the fine control of digestive functions. This forms a complex network, which includes several types of neurons, as well as glial cells. Despite extensive studies, a comprehensive classification of these neurons is still lacking. The complexity of ENS is magnified by a multiple control of the central nervous system, and bidirectional communication between various central nervous areas and the gut occurs. This lends substance to the complexity of the microbiota–gut–brain axis, which represents the network governing homeostasis through nervous, endocrine, immune, and metabolic pathways. The present manuscript is dedicated to identifying various neuronal cytotypes belonging to ENS in baseline conditions. The second part of the study provides evidence on how these very same neurons are altered during Parkinson’s disease. In fact, although being defined as a movement disorder, Parkinson’s disease features a number of degenerative alterations, which often anticipate motor symptoms. Among these, the GI tract is often involved, and for this reason, it is important to assess its normal and pathological structure. A deeper knowledge of the ENS is expected to improve the understanding of diagnosis and treatment of Parkinson’s disease.
Association of Dephosphorylated-Uncarboxylated Matrix Gla Protein and Risk of Major Bleeding in Patients Presenting with Acute Myocardial Infarction
Authors: Bilalic Admira , Kurir Tina Ticinovic , Borovac Josip A. , Kumric Marko , Supe-Domic Daniela , Vilovic Marino , Martinovic Dinko , Bozic Josko
Published: July 2021

The “Can Rapid risk stratification of Unstable angina patients Suppress ADverse outcomes with Early implementation of the ACC/AHA guidelines” (CRUSADE) score emerged as a predictor of major bleeding in patients presenting with the acute coronary syndrome. On the other hand, previous studies established the association of dephosphorylated-uncarboxylated Matrix Gla protein (dp-ucMGP) and vitamin K, as well as their subsequent impact on coagulation cascade and bleeding tendency. Therefore, in the present study, we explored if dp-ucMGP plasma levels were associated with CRUSADE bleeding score. In this cross-sectional study, physical examination and clinical data, including plasma dp-ucMGP levels, were obtained from 80 consecutive patients with acute myocardial infarction (AMI). A significant positive correlation was found between CRUSADE bleeding score and both dp-ucMGP plasma levels (r = 0.442, p < 0.001) and risk score of in-hospital mortality (r = 0.520, p < 0.001), respectively. In comparing the three risk groups of risk for in-hospital bleeding, the high/very high-risk group had significantly higher dp-ucMGP levels from both very low/low group (1277 vs. 794 pmol/L, p < 0.001) and the moderate group (1277 vs. 941 pmol/L, p = 0.047). Overall, since higher dp-ucMGP levels were associated with elevated CRUSADE score and prolonged hemostasis parameters, this may suggest that there is a biological link between dp-ucMGP plasma levels and the risk of bleeding in patients who present with AMI.
Water-Based Lubricants: Development, Properties, and Performances
Authors: Rahman Md Hafizur , Warneke Haley , Webbert Haley , Rodriguez Joaquin , Austin Ethan , Tokunaga Keli , Rajak Dipen Kumar , Menezes Pradeep L.
Published: July 2021

Water-based lubricants (WBLs) have been at the forefront of recent research, due to the abundant availability of water at a low cost. However, in metallic tribo-systems, WBLs often exhibit poor performance compared to petroleum-based lubricants. Research and development indicate that nano-additives improve the lubrication performance of water. Some of these additives could be categorized as solid nanoparticles, ionic liquids, and bio-based oils. These additives improve the tribological properties and help to reduce friction, wear, and corrosion. This review explored different water-based lubricant additives and summarized their properties and performances. Viscosity, density, wettability, and solubility are discussed to determine the viability of using water-based nano-lubricants compared to petroleum-based lubricants for reducing friction and wear in machining. Water-based liquid lubricants also have environmental benefits over petroleum-based lubricants. Further research is needed to understand and optimize water-based lubrication for tribological systems completely.
Mechanical Behavior Investigation of Reclaimed Asphalt Aggregate Concrete in a Cold Region
Authors: Xu Wenyuan , Li Wei , Ji Yongcheng
Published: July 2021

Recycled construction and demolition (C&D) waste can reduce the rebuild cost, and is environmentally friendly when recycled asphalt pavement (RAP) aggregate constitutes the main part. This paper investigated the mechanical performance of RAP concrete, and the applicability of RAP in road base layers also was discussed. Several mechanical laboratory tests were selected, including the unconfined compressive-strength, splitting-strength, and compressive-resilience modulus tests. The RAP concrete had a good road performance in a cold region, which was proved by the temperature-shrinkage test, dry-shrinkage test, freeze–thaw-cycle test, and water-stability test. Based on various cement dosages from 3.5% to 5.5% in RAP concrete mix design, three RAP aggregate replacement ratios (30%, 40%, and 50%) were selected to study the variation of mechanical properties with increasing curing time, and the optimal aggregate substitute ratio was determined. A scanning electron microscope (SEM) was used to observe the inner-structure interface between the asphalt binder and cement stone. A numerical model is presented to simulate the RAP compressive strength with respect to the effect of multiple parameters. The research results can provide a technical reference for RAP use in the reconstruction and expansion of low-grade highway projects.