bluuebunny/crossref_metadata_2025 huggingface dataset:
Recommending Turmeric Variety for Higher Production Using Interval-Valued Fuzzy Soft Set Model and PSO
Authors: Mohanty R. K. , Tripathy B. K.
Published: April 2021

Soft set is one of the latest mathematical models to handle uncertainty. In a soft set, every element of its parameter set is associated with a subset of the universe of discourse under consideration. In recent times, soft set and its hybrid models have been used extensively to handle decision making problems with uncertain data. It's established that an appropriate hybrid model works better than its basic components. In this article, an algorithm is proposed that is used to recommend the best variety of turmeric having a given set of parameters using Interval valued fuzzy soft sets. Most importantly, the priorities of parameters are taken as fuzzy interval values so that higher uncertainty can be handled properly. Reduction of parameters helps in getting down the complexity of the process under consideration. A metaheuristic optimization technique is a better option to handle these kinds of problems. The authors use particle swarm optimization (PSO) to achieve parameter reduction.
Novel Interdiffusion Analysis in Multicomponent Alloys - Part 1: Application to Ternary Alloys
Authors: Afikuzzaman Mohammad , Belova Irina V. , Murch Graeme E.
Published: April 2021

A novel study of interdiffusion analysis in multicomponent alloys is presented. A custom written Matlab fitting program (MFP) is used as the main tool for the present study. The interdiffusion matrices are obtained using a newly developed mathematical approach based on the fitting into the closed form solution for the composition profiles. Overall, the new fitting method gives very good outcomes and allows the probing of multiple solutions (validated by back tests) that exist when a single diffusion couple is used in a multicomponent system. An important finding of the present investigations is that small differences in composition profiles may lead to large differences in the interdiffusion coefficients.
Amelioration of Graft-versus-Host Disease by Exopolysaccharide from a Commensal Bacterium
Authors: Kalinina Olga , Talley Sarah , Zamora-Pineda Jesus , Paik Wonbeom , Campbell Edward M. , Knight Katherine L.
Published: April 2021

Acute graft-versus-host disease (aGvHD) is a severe, often lethal, complication of hematopoietic stem cell transplantation, and although prophylactic regimens are given as standard pretransplantation therapy, up to 60% of these patients develop aGvHD, and require additional immunosuppressive intervention. We treated mice with a purified probiotic molecule, exopolysaccharide (EPS) from Bacillus subtilis, shortly before and after induction of aGvHD and found that, whereas only 10% of control mice survived to day 80, 70% of EPS-treated mice survived to 80 d. EPS treatment of donor-only mice resulted in ∼60% survival. Using a biosensor mouse model to assess inflammation in live mice during aGvHD, we found that EPS prevented the activation of alloreactive donor T cells. In vitro, EPS did not affect T cells directly but, instead, induced bone marrow–derived dendritic cells (BMDCs) that displayed characteristics of inhibitory dendritic cells (DCs). Development of these BMDCs required TLR4 signaling through both MyD88 and TRIF pathways. Using BMDCs derived from IDO knockout mice, we showed that T cell inhibition by EPS-treated BMDCs was mediated through the suppressive effects of IDO. These studies describe a bacterial molecule that modulates immune responses by inducing inhibitory DCs in a TLR4-dependent manner, and these cells have the capacity to inhibit T cell activation through IDO. We suggest that EPS or EPS-treated DCs can serve as novel agents for preventing aGvHD.
Autoreactivity of Peripheral Helper T Cells in the Joints of Rheumatoid Arthritis
Authors: Sakuragi Takahide , Yamada Hisakata , Haraguchi Akihisa , Kai Kazuhiro , Fukushi Jun-ichi , Ikemura Satoshi , Akasaki Yukio , Fujiwara Toshifumi , Tsushima Hidetoshi , Tsutsui Tomoko , Kondo Masakazu , Yoshikai Yasunobu , Okada Seiji , Nakashima Yasuharu
Published: April 2021

Autoreactive CD4 T cells are thought to play pivotal roles in the pathogenesis of rheumatoid arthritis (RA). Recently, a subset of CD4 T cells that express high levels of programmed death-1 (PD-1) but are distinct from follicular helper T cells have been identified in the joints of RA patients and named peripheral helper T (Tph) cells. Because PD-1 is expressed on T cells chronically stimulated with the Ags, we tested a hypothesis that Tph cells are the pathogenic autoreactive CD4 T cells in RA. We found that human Tph cells in RA joints produce proinflammatory effector cytokines, including IFN-γ, TNF-α, and GM-CSF, in addition to B cell–helping cytokines, such as IL-21 and CXCL13. Flow cytometric analysis showed different bias of TCR Vβ usage between PD-1high Tph cells and PD-1low/neg CD4 T cells, including Th1 cells, in the joint or memory CD4 T cells in the peripheral blood, whereas there was little difference between the latter two subsets. In line with this, deep sequencing of TCR demonstrated an overlap of expanded clones between peripheral blood memory CD4 T cells and PD-1low/neg CD4 T cells but not Tph cells in the joint. Interestingly, Tph cells preferentially exhibited autologous MLR in vitro, which required recognition of self–MHC class II and was pronounced by blocking PD-1 signaling. Taken together, these results suggest that Tph cells are the pathogenic autoreactive CD4 T cells in RA, which expand locally in the joints and are regulated by PD-1 signaling.
Longitudinal Peripheral Blood Transcriptional Analysis Reveals Molecular Signatures of Disease Progression in COVID-19 Patients
Authors: Yan Qihong , Li Pingchao , Ye Xianmiao , Huang Xiaohan , Feng Bo , Ji Tianxing , Chen Zhilong , Li Feng , Zhang Yudi , Luo Kun , Chen Fengjuan , Mo Xiaoneng , Wang Jianhua , Feng Liqiang , Hu Fengyu , Lei Chunliang , Qu Linbing , Chen Ling
Published: April 2021

Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with some patients developing severe illness or even death. Disease severity has been associated with increased levels of proinflammatory cytokines and lymphopenia. To elucidate the atlas of peripheral immune response and pathways that might lead to immunopathology during COVID-19 disease course, we performed a peripheral blood RNA sequencing analysis of the same patient’s samples collected from symptom onset to full recovery. We found that PBMCs at different disease stages exhibited unique transcriptome characteristics. We observed that SARS-CoV-2 infection caused excessive release of inflammatory cytokines and lipid mediators as well as an aberrant increase of low-density neutrophils. Further analysis revealed an increased expression of RNA sensors and robust IFN-stimulated genes expression but a repressed type I IFN production. SARS-CoV-2 infection activated T and B cell responses during the early onset but resulted in transient adaptive immunosuppression during severe disease state. Activation of apoptotic pathways and functional exhaustion may contribute to the reduction of lymphocytes and dysfunction of adaptive immunity, whereas increase in IL2, IL7, and IL15 may facilitate the recovery of the number and function of lymphocytes. Our study provides comprehensive transcriptional signatures of peripheral blood response in patients with moderate COVID-19.
Patient perceived quality of nursing care in hemodialysis: A meta-synthesis
Authors: Balouchi Abbas , Ebadi Abbas , Parvizy Soroor , Sharif Nia Hamid
Published: April 2021

This study was done with the purpose of clarifying the concept of patient perceived quality of nursing care in hemodialysis. In this meta-synthesis study, qualitative studies was searched in the four interntional databases from January 1st, 2000 to December 30th, 2019. The keywords used were: nursing care quality, and hemodialysis. Studies which had been done with the purpose of understanding the patient perceived quality of nursing care concept aomg hemodialysis patients were included. Two researchers were evaluate the quality of included studies separately using JBI tool, required information were extracted using the designed table. The main themes in the structure dimension include Sufficient Human resource, quality of equipment, financial support from patient and quality of the patient care environment. Process dimension consisted of continuous monitoring of the syndromes, effective patient education, efficient care, effective therapeutic relationship, and patient’s empowerment and participation in the process, and in the outcome dimension included high dialysis adequecy, patient burnout reduction, and increased patient satisfaction from services. The results of the study indicated a comprehensive, deep and interactive dimensions about the concept of nursing quality of care.
Terapie digitali e sanità digitalizzata
Authors: Spagnolo Antonio G.
Published: April 2021

Non disponibile.
Comparison of the ulnar nerve blockade between intertruncal and corner pocket approaches for supraclavicular block: a randomized controlled trial
Authors: Jo Yumin , Park Jiho , Oh Chahyun , Chung Woosuk , Song Seunghyun , Lee Jieun , Kang Hansol , Ko Youngkwon , Kim Yoon-Hee , Hong Boohwi
Published: April 2021

Background: The corner pocket (CP) approach for supraclavicular block (SCB) prevents ulnar nerve (UN) sparing due to needle proximity to the lower trunk. Improved ultrasound resolution has suggested that the intertruncal (IT) approach is a suitable alternative method. We compared efficiency of these two approaches on the UN blockade.Methods: Sixty patients were randomized to undergo SCB using the ultrasound-guided CP or IT approach. For lower trunk blockade, 10 ml of local anesthetic agents (1 : 1 mixture of 0.75% ropivacaine and 1% lidocaine) were injected in the CP (CP approach) or between the lower and middle trunks (IT approach). Additional 15 ml was injected identically to block the middle and upper trunks in both groups. Sensory and motor blockade was evaluated after intervention. Results: Complete sensory blockade (75.9% [22/29] vs. 43.3% [13/30], P = 0.023) and complete motor blockade (82.8% [24/29] vs. 50.0% [15/30], P = 0.017) of the UN at 15 min after SCB were significantly more frequent in the IT than in the CP group. Sensory block onset time of the UN was significantly shorter in the IT compared to the CP group (15.0 [10.0; 15.0] min vs. 20.0 [15.0; 20.0] min; P = 0.012). Conclusions: The IT approach provided a more rapid onset of UN blockade than the CP approach. These results suggest that the IT approach is a suitable alternative to the CP approach and can provide faster surgical readiness.
Predictors of acceptance and willingness to pay for the COVID-19 vaccine in the general public of India
Authors: Goruntla Narayana , Chintamani Sai Harshavardhan , Bhanu P , Samyuktha S , Veerabhadrappa Kasturi Vishwanathasetty , Bhupalam Pradeepkumar , Ramaiah Jinka Dasaratha
Published: April 2021

Objective:

To determine the predictors of acceptance and willingness to pay (WTP) for the COVID-19 vaccine among the Indian public and to provide insights for future demand forecasts and pricing considerations.

Methods:

A nationwide, web-based, self-administered, cross-sectional survey was conducted from 5 to 20 October 2020. The health belief model (HBM) approach was used as a theoretical framework to assess the predictors of acceptance of and WTP for the COVID-19 vaccine.

Results:

Of 2 480 respondents, 2 451 completed the online survey, yielding a response rate of 98.8%. Participants who participated in the survey had diverse demographics in terms of their location, educational level, occupation type, and family income. Among 2 451 respondents, the majority (89.3%) intended to receive the COVID-19 vaccine. Respondents with high perceived benefits of COVID-19 vaccination, such as reduction in worry (OR 5.87; 95% CI 4.39-7.96) and sickness (OR 4.31; 95% CI 3.31-5.62), showed higher intention to receive the vaccine. However, respondents with a high perception of the side effects and barriers to vaccination (OR 0.36; 95% CI 0.25-0.54) and vaccine shortage (OR 0.58; 95% CI 0.41-0.81) showed lower intention to receive the vaccine. The majority (2 162, 88.21%) of respondents were willing to pay an amount of INR: 500-1 000 or USD: 6.81-13.62 for a dose of COVID-19 vaccine, with a median (Q1, Q3) of INR: 500 (500, 1 000) or USD: 6.81 (6.81, 13.62). The higher marginal WTP for the COVID-19 vaccine was influenced by advanced age, marital status, female sex, intermediate educational background, high family income, fair or poor perceived health status, and no affordable barriers.

Conclusions:

The majority of respondents intended to receive the COVID-19 vaccine. Healthcare interventions focusing on HBM constructs and demographic predictors associated with low intention to receive the vaccine can be effective in enhancing the coverage of the COVID-19 vaccine. The findings of this study provide guidance for the future price considerations of the COVID-19 vaccine.
COVID-19 vaccination intention among healthcare workers in Vietnam
Authors: Huynh Giao , Tran Thien Thuan , Nguyen Thi Ngoc , Pham Le An
Published: April 2021

Objective:

To assess the acceptance of coronavirus disease (COVID-19) vaccine among healthcare workers at two general hospitals in Vietnam when it is available.

Methods:

A cross-sectional study was conducted using a convenience sampling from January to February 2021 among 410 healthcare workers at two general hospitals in Vietnam via a self-administered questionnaire. A multivariable regression analysis was performed to determine predictors of vaccine acceptance including the demographic factors, COVID-19 knowledge, and vaccine beliefs based on the domains of Health Belief Model.

Results:

Among 410 healthcare workers, 76.10% showed vaccination willingness. Predictors of acceptance were determined that the group reporting as “vaccine acceptance” was more likely to be positive towards the perceived susceptibility and severity of COVID-19 (OR 2.45; 95% CI 1.48-4.06, P<0.05), perceived benefits of vaccination, and cues to action (OR 4.36; 95% CI 2.35-8.09, and OR 5.49; 95% CI 2.84-10.61, respectively, all P<0.001), but less likely to have the perceived barriers to vaccination (OR 0.19; 95% CI 0.09-0.38; P<0.001) compared with the no acceptance group. Besides, people who had a good knowledge regarding the severity of illness were 3.37 times more likely to have identified as vaccine acceptance (OR 3.37; 95% CI 1.04-10.86, P<0.05). The demographic factors were also associated with willingness to receive the vaccine, with participants who were staff and received COVID-19 information from relatives were less likely to accept the vaccine over those who were doctors and not receiving information from relatives (OR 0.36; 95% CI 0.13-0.96, and OR 0.37; 95% CI 0.17-0.78, respectively, all P<0.05).

Conclusions:

A rate of willingness to get vaccinated against COVID-19 was relatively high with discrepancies between occupation, receiving information from relatives, knowledge toward the severity of illness, and the elements of Health Belief Model. The findings will provide information for the management authorities to develop relevant interventions to promote COVID-19 vaccination uptake.
Severe dengue associated with aseptic meningitis, acute kidney injury, and sudden sensorineural hearing loss
Authors: Diniz Renan GM , dos Santos Elaine C , Chagas Gabriel CL , Daher Elizabeth F
Published: April 2021

Rationale:

Dengue is the most important human arboviral disease. Neurological manifestations occur rarely in dengue. To the best of our knowledge, there is only one reported case of dengue-associated sudden sensorineural hearing loss (SSNHL) in Brazil untill now.

Patient concerns:

A 42-year-old man was presented to the emergency department with unconsciousness and generalized tonic-clonic seizures.

Diagnosis:

Dengue-associated aseptic meningitis, acute kidney injury, and SSNHL.

Interventions:

The patient was treated with anticonvulsants and thiamine and underwent mechanical ventilation. He received combined ceftriaxone and acyclovir, which were later switched to meropenem, acyclovir and ampicillin empirically until culture results became available. He also required hemodialysis and plasmapheresis sessions and fresh frozen plasma and buffy coat transfusions until definitive diagnosis.

Outcomes:

The patient was discharged after improvement of his general condition and of his blood test results, but hearing loss remained. A six-month follow-up visit showed persistent deafness.

Lessons:

Dengue should be included in the differential diagnosis of patients from dengue-endemic areas presenting an acute febrile disease with neurological manifestations. To the best of our knowledge, this is the second reported case of dengue-associated SSNHL, suggesting an association between dengue and development of SSNHL.
Protective effects of rice bran hydrolysates on heart rate variability, cardiac oxidative stress, and cardiac remodeling in high fat and high fructose diet-fed rats
Authors: Senaphan Ketmanee , Kukongviriyapan Upa , Suwannachot Pisit , Thiratanaboon Geerasak , Sangartit Weerapon , Thawornchinsombut Supawan , Jongjareonrak Akkasit
Published: April 2021

Objective:

To examine the ameliorative effect of rice bran hydrolysates (RBH) on metabolic disorders, cardiac oxidative stress, heart rate variability (HRV), and cardiac structural changes in high fat and high fructose (HFHF)-fed rats.

Methods:

Male Sprague-Dawley rats were daily fed either standard chow diet with tap water or an HFHF diet with 10% fructose in drinking water over 16 weeks. RBH (500 and 1 000 mg/kg/day) was orally administered to the HFHF-diet-fed rats during the last 6 weeks of the study period. At the end of the treatment, metabolic parameters, oxidative stress, HRV, and cardiac structural changes were examined.

Results:

RBH administration significantly ameliorated metabolic disorders by improving lipid profiles, insulin sensitivity, and hemodynamic parameters. Moreover, RBH restored HRV, as evidenced by decreasing the ratio of low-frequency to high-frequency power of HRV, a marker of autonomic imbalance. Cardiac oxidative stress was also mitigated after RBH supplementation by decreasing cardiac malondialdehyde and protein carbonyl, upregulating eNOS expression, and increasing catalase activity in the heart. Furthermore, RBH mitigated cardiac structural changes by reducing cardiac hypertrophy and myocardial fibrosis in HFHF-diet-fed rats.

Conclusions:

The present findings suggest that consumption of RBH may exert cardioprotective effects against autonomic imbalances, cardiac oxidative stress, and structural changes in metabolic syndrome.
Aloin attenuates chronic constriction injury-induced neuropathic pain in rats by inhibiting inflammatory cytokines and oxidative stress
Authors: Kale Aarti S. , Wadkar Avinash R. , Mahajan Umesh B. , Birari Lalit A. , Belemkar Sateesh , Goyal Sameer N. , Ojha Shreesh , Surana Sanjay J. , Patil Chandragouda R. , Patil Kalpesh R.
Published: April 2021

Objective:

To investigate the effect of aloin against chronic constriction injury (CCI)-induced neuropathic pain in rats.

Methods:

Rats were randomly divided into 7 groups: Group I (normal control), Group II (sham-operated), Group III (CCI control) and Group IV, V, VI, and VII, which underwent CCI surgery and then were administered with aloin (5 mg/kg, p.o.; 25 mg/kg, p.o.; 125 mg/kg, p.o.) and gabapentin (50 mg/kg, p.o.), respectively for 14 days. Peripheral neuropathy was induced by silk ligatures (4-0) loosely placed around the sciatic nerve. Nociceptive thresholds against mechanical stimuli (Von-Frey filaments) and thermal stimuli (12 °C and 40 °C) were measured at mid-plantar paw region ipsilateral to the compressed nerve on day-3, 7, 11, and 14. The concentration of cytokines including tumor necrosis factor-α (TNF-α), interleukin-6, and interleukin-1β was estimated at day-7. At day 14, motor nerve conduction velocity was determined under urethane anesthesia (1.25 g/kg). Oxidative stress parameters (malondiadehyde, glutathione, catalase, and superoxide dismutase) were estimated in sciatic nerve homogenates at day 14. Representative nerve samples were processed for histological investigations.

Results:

Aloin significantly reduced CCI-induced mechanical and thermal allodynia. It also improved motor nerve conduction velocity and decreased oxidative stress in nerve tissues. In addition, it decreased pro-inflammatory cytokine levels and restored the histoarchitecture of compressed sciatic nerve.

Conclusions:

Aloin mitigates CCI-induced neuropathic pain in rats by inhibiting oxidative stress and pro-inflammatory cytokines in the afflicted sciatic nerve.
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling
Authors: Choi Yung Hyun
Published: April 2021

Objective:

To investigate the effect of honokiol on oxidative damage in HaCaT human keratinocytes.

Methods:

HaCaT cells were exposed to hydrogen peroxide (H2O2), following pretreatment with various concentrations of honokiol. The alleviating effects of honokiol on HaCaT cell viability and cell death, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of adenosine triphoaphate production against H2O2 were investigated. Western blotting analysis was used to analyze the expression levels of specific proteins.

Results:

Honokiol suppressed H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS accumulation. Honokiol also prevented apoptosis by inhibiting loss of mitochondrial membrane potential and release of cytochrome c from the mitochondria into the cytosol, decreasing the Bax/Bcl-2 ratio, and reducing the activity of caspase-3 in H2O2-stimulated HaCaT cells. In addition, honokiol attenuated H2O2-induced reduction of adenosine triphosphate content, and activation of AMP-activated protein kinase (AMPK) was markedly promoted by honokiol in H2O2-stimulated cells. Importantly, the anti-apoptosis and anti-proliferative activity of honokiol against H2O2 was further enhanced by adding an activator of AMPK, indicating that honokiol activated AMPK in HaCaT keratinocytes to protect against oxidative damage.

Conclusions:

The present results indicate that honokiol may be useful as a potential therapeutic agent against various oxidative stress-related skin diseases.
A Review of Medical Image Segmentation Algorithms
Authors: Ramesh K.K.D. , Kumar G. Kiran , Swapna K. , Datta Debabrata , Rajest S. Suman
Published: April 2021

INTRODUCTION: Image segmentation in medical physics plays a vital role in image analysis to identify the affected tumour. The process of subdividing an image into its constituent parts that are homogeneous in feature is called Image segmentation, and this process concedes to extract some useful information. Numerous image segmentation techniques have been developed, and these techniques conquer different restrictions on conventional medical segmentation techniques. This paper presents a review of medical image segmentation techniques and statistical mechanics based on the novel method named as Lattice Boltzmann method (LBM). The beauty of LBM is to augment the computational speed in the process of medical image segmentation with an accuracy and specificity of more than 95% compared to traditional methods. As there is not much information on LBM in medical physics, it is intended to present a review of the research progress of LBM.OBJECTIVE: As there is no review paper on the research progress of the LB method, this paper presents a review with an objective to give some thought regarding the different segmentation for medical image and novel LB method to advance interest for future investigation and exploration in medical image segmentation.METHODS: This paper in attendance a short review of medical image segmentation techniques based on Thresholding, Region-based, Clustering, Edge detection, Model-based and the novel method Lattice Boltzmann method (LBM).CONCLUSION: In this paper, we outlined various segmentation techniques applied to medical images, emphasize that none of these problem areas has been acceptably settled, and all of the algorithms depicted are available for broad improvement. Since LBM has the benefits of speed and adaptability of modelling to guarantee excellent image processing quality with a reasonable amount of computer resources, we predict that this method will become a new research hotspot in image processing.
Comparative analysis between the chlorination and ultraviolet radiation methods for the disinfection of bacteria-contaminated water
Authors: Ritt Barbara Santos , Fernandes André Luís Teixeira , Thedei Júnior Geraldo
Published: April 2021

The quality of water for human consumption has been a concern for large organizations and researchers, since the disinfection process, a step of great importance in water treatment plants, has not achieved its proper purpose. There have been frequent reports of the ineffectiveness of bacteria inactivation by the use of chlorine, which is the most used in Brazil, and also due to the formation of by-products such as trihalomethanes and haloacetic acids that are considered toxic to human health. The method of disinfection by ultraviolet radiation (UV) has been widely explored, due to its effectiveness in inactivating a wide variety of pathogens, in addition to the absence of by-product formation. The combined chlorine+UV process as chemical and physical disinfectants, respectively, is complementary and essential when looking for the safety of water for human consumption. The objective of this research was to statistically analyze the performance of disinfectants, chlorine and UV radiation, both separately and together, by inactivating the bacteria Escherichia coli and Pseudomonas aeruginosa and then incubating for 48 hours after having received the treatments. The statistical method showed that chlorine and UV obtained the same significance of 100% bacteria inactivation, and that within the determined time of 48 hours after the treatments the bacteria did not show reactivation. Thus, the study affirmed the usefulness of applying UV radiation as a complement to systems that use chlorine, with a special focus on residential water systems and supply.
Case Report: Atypical Presentation of Visceral Leishmaniasis: Two Cases from Northwest Ethiopia
Authors: Mohammed Rezika , Fikre Helina , Mekonnen Tigist , Abebe Bewketu , Yeshanew Arega , Diro Ermias , van Griensven Johan
Published: April 2021

Human visceral leishmaniasis (VL) is a life-threatening disease caused by protozoan parasites belonging to the Leishmania donovani complex. Atypical cases of leishmaniasis and HIV coinfection have been documented in case reports, mostly associated with gastrointestinal tract, kidney, and skin involvement. We report two VL cases with atypical localizations not reported from east Africa before, both diagnosed and treated at the Leishmaniasis Research and Treatment Center, Gondar, Ethiopia. The first case was an HIV-infected patient with scrotal and penile involvement. Leishmania parasites were detected in the spleen and the scrotum. The second case was an immunocompetent individual with esophageal, laryngeal, and pharyngeal involvement and facial lesions. Leishmania parasites were detected in the spleen, skin, and esophageal biopsies. Current evidence suggests atypical presentation can occur in patients irrespective of their HIV status. Therefore, we suggest a high index of suspicion for VL among clinicians working in endemic areas of Ethiopia.
A unique quadrifurcation of the sciatic nerve in the lower leg
Authors: Russa Afadhali D. , Fabian Flora M.
Published: April 2021

Sciatic nerve is the largest nerve of the body supplying the entire posterior aspect of the lower limb. Taking its origin from the lumbosacral plexus, the nerve divides into its terminal branches at the superior angle of the popliteal fossa. Variant division patterns of the nerve especially those occurring in the thigh and the popliteal fossa are common. Divisions of the nerve occurring in the leg are rare. Even much rarer are terminations involving multiple branching patterns. Variations in the course and distribution of the sciatic nerve are of great importance in neurology, orthopedics, rehabilitation, anesthesia and many other clinical procedures. In the present study, we report a unique quadrifurcation termination of the sciatic nerve occurring deep in the distal half of the leg as observed during routine dissection by the medical students. Due to its variant muscular innervations, quadrifurcation and low divisions of the sciatic nerve may be of great importance in procedures such fracture management of the leg and foot, physiotherapy, limb amputation and in sports medicine. Embryologically, late separation of the common nerve sheath into individual terminal branches may lead to the lower division of the nerve.KEY WORDS: Sciatic nerve, quadrifurcation, lower division, fracture
Tackling the grassroots: appraisal of career choice and prospects of the students at a medical school in Tanzania
Authors: Russa Afadhali D. , Mligiliche Nuru. L. , Malya Rehema C.
Published: April 2021

Tanzania has one of the world’s worst doctor-to-population ratio. Ironically, the number of medical graduates who do not practice medicine remains very high. Lack of interest and commitment of the young doctors may greatly contribute to the huge number of non-practicing doctors. We assessed medical students’ career views from interest and choice during childhood, their current learning motivations, future career expectations and interest to work in the academia. One hundred and ninetyfive students halfway their first year of medical school complete a self-administered questionnaire with a verbal consent sought from each respondent. Four in every ten students had made their decision to become a doctor before or during primary school. Over 5 out of 10 students made their choice during secondary school. Majority chose medicine due to altruistic and humanity reasons and would prefer work in clinical practice in the hospital. Very few would prefer a faculty job. Knowing about the human body, controlling and managing diseases are the main motivating factors. ‘Too much to learn’ and ‘tight schedules’ were the most aspect and deterrent factors during the medical school and the medical profession in general. Attaining a higher academic honour such as a professorship would not inspire students into the academia. Majority of students make their medical career choice early in life driven by altruistic reasons. Poor learning environments disenchant them from the passion for the career. More studies are needed to assess and improve the training in medicine.Keywords: Medical students, motivation, career prospects, Tanzania
What is the origin of the labyrinthine artery among black Kenyans?
Authors: Ogeng’o Julius A. , Olabu Beda O. , Mwachaka Phillip M. , Ominde Beryl S. , Inyimili Martin I.
Published: April 2021

Origin of labyrinthine artery is important because it influences the presentation of occlusion of anterior inferior cerebellar and basilar arteries. It shows ethnic and geographical variation, but there is no data from black African populations. This study, therefore examined the pattern of origin of labyrinthine artery in adult black Kenyans. Three hundred and fourty six arteries from one hundred and seventy-three formalin fixed brains were examined by dissection at the Department of Human Anatomy University of Nairobi, Kenya. Labyrinthine artery arose from basilar artery in 260 (75.1%); as common trunk with anterior inferior cerebellar artery in 48 (13.9%) and from the latter in 38 (11.0%) of cases. There was no side and gender difference in the pattern of origin. This implies that majority of labyrinthine arteries arise from basilar artery, different from that in oriental, Indo-Asian and Caucasian populations, in which it arises from the anterior inferior cerebellar artery. Preoperative evaluation of basilar artery branching is recommended.Keywords: labyrinthine artery, origin, Kenyan, Basilar, AICA
Long term effects of formaldehyde preservation on subsequent bone maceration procedures: a comparative study between cold and hot water maceration
Authors: Odukoya Ayodeji S.O. , Ajani Oluwaseun , Adelodun Taiye S.
Published: April 2021

This study was conducted to compare the effectiveness of cold and hot water maceration techniques for the development of bone specimens from cadavers. Three already dissected bodies of both sexes (two males and a female) obtained from the Department of Anatomy, Bowen University, Iwo, Osun State, Nigeria were used. The male bodies were labelled as A and B and the female as C. Before maceration, the heights of the bodies were taken to be 165 cm, 170 cm and 160 cm for A, B and C respectively. The bodies were preserved in formalin for 4 years before being used. Hot water maceration involves cooking bones in water. Bodies A and C were used in this method. While cold water maceration is simply by soaking the body in water until all the remaining flesh was removed. Hot water maceration showed changes in color of the bones, shrinkage of the bones, oily substances were seen on the surface of the chloroform during degreasing and the bones were very soft after bleaching but got harder after drying with sunlight. Cold water maceration on the other hand kept the bones white and maintained their integrity though it required a lot of time. Small bones had their flesh removed quickly in few weeks, while larger and longer bones took months. We conclude that cold water maceration done properly, results in cleaner bones and better morphological outcomes than hot water method though it requires much time.KEYWORDS: Maceration, Formaldehyde, Comparative, Bones, Hot water, Cold water
Homo sapiens are bilaterally symmetrical but not with toe length and toe-length ratios among Nigerians
Authors: Alabi A.S. , Oladipo G.S. , Didia B.C. , Aigbogun E.O. , Akintunde K.A.
Published: April 2021

The concept of bilateral symmetry in organisms involves the division of the body plan along a plane that splits the animal's body into right and left sides that are mirror images of each other. However can this be true for toe lengths and ratios? The present study evaluates the symmetric differences in toe length and toe-length ratios among the three major ethnic groups in Nigeria. A total of 1574 adult Nigerian Hausas (664), Igbos (420) and Yorubas (490) of equal sex were included in the study. Aged between 18-65 years, were randomly selected from various states. Written informed consent was taken from each participant, sample size was determined by proportion, using Cochran formulae for infinite population. A digital Vernier caliper was used to obtain direct linear measurements of the toe length of both feet; hallux (1T), second toe (2T), third toe (3T), fourth toe (4T), and the fifth toe (5T). Ten (10) possible toe-length ratios were also determined and named as follows; 1T/2T, 1T/3T, 1T/4T, 1T/5T, 2T/3T, 2T/4T, 2T/5T, 3T/4T, 3T/5T, and 4T/5T. Data were analyzed using SPSS (IBM®Armonk, New York, USA) and Minitab V17 (Minitab® Inc. State College, Pennsylvania) statistical software. Paired-sample t-test was used to evaluate symmetry; Pearson’s Correlation was used for inter-prediction of the toes and ratios. Confidence level was set at 95%; as P-values ≤0.05 were considered significant. The result showed that some of the toe length of both feet were significantly assymetrical among the studied ethnic groups; Hausa males (1T df=1.35 and 5T df=0.55; P<0.05) and females (1T and 3T; (1T df=0.27, 3T df=0.47) P<0.001), Igbo males (1T, 3T and 4T(1T df=0.47, 3T, df=-0.53, 4T df=-0.58) ; P<0.05 and females 1T and 3T(1T df=0.40, T3 df=-0.64) ; P<0.01) and Yoruba males(1T, 2T, 3T and 5T(1T df=1.29, 2T df=0.47, 3T df=0.15, and 5T df=0.55,); P<0.001) and females (1T and 5T(1T df=0.77; 5T df=0.23) ;P<0.001). The toe-length ratios also displayed symmetrical differences for Nigerian male population; 1T:2T [t=3.78, P<0.001], 1T:3T [t=6.27, P<0.001], 1T:4T [t=6.04, P<0.001], 1T:5T [t=2.43, P=0.015], 2T:3T [t=2.23, P=0.026], 3T:5T [t=-3.78, P<0.001] and 4T:5T [t=-3.77, P<0.001].For the female population, all ratios were not significantly different except for1T:4T [t=2.56, P=0.011]. However, both feet displayed significant positive association for co-estimation of the corresponding toe length and toe-length ratios (r-values ranging from 0.20-0.70 at P<0.05).Ethnic and sex specific asymmetry were observed in the toe length and toe-length ratios; thus suggesting that anthropometrically, organisms are not completely bilaterally symmetrical.Keywords: Asymmetry, Bilateral symmetry, Toe length, Toe-length ratio, Ethnic groups
In vivo evidences of Curcuma longa on oxidative stress in STZ-induced diabetes on sperm parameters in male wistar rats
Authors: Olanrewaju A.J. , Olatunji S.Y. , Owolabi J.O. , Oluwatosin A.T. , Amaechi W.C. , Desalu A.B.O.
Published: April 2021

Several conditions such as chemotherapy and toxins can interfere with spermatogenesis and reduce sperm quality and production. In the case where natural antioxidant response cannot manage oxidative stress and free radical overload, oxidative damage occurs and this begins the genesis of many diseases of which diabetes is one of them. This has awakened the interest of researchers to the use of an alternative source of medicine and herbal medicine. Medicinal use of Curcuma longa dates back to ancient China and India; its constituents are stated to have anti-hepatotoxic, anti-inflammatory, stimulant, and antioxidant and used since ancient time as medicinal and nutritive origins knowing to possess androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant Curcuma longa possible has a useful effect on spermatogenesis and sperm parameters. Wistar male rats (n=24) were allocated into six groups, positive control (n=4), diabetic control (n=4) and experimental groups (n=20), that subdivided into groups of 4 that received treatment of Curcuma longa rhizome powder with or without STZ-induced diabetes in the dosages (25 and 100mg/kg/day) for 21 consecutive days. In twenty-second day, the testes were removed and semen was collected from epididymis and prepared for analysis. The percentage of sperm viability and motility in the treatment groups increased mildly with a significance of: (p<0.05) in comparison to control group and with the diabetic group being critically lower than those in control group. This suggested that Curcuma longa may be promising in enhancing sperm health parameters.Keywords: Turmeric, oxidative stress, semen, STZ-induced diabetes, antioxidant
Alcohol-pentazocine combination: implication on the cytoarchitectural profile of the medial prefrontal cortex and cerebellum of juvenile male rats
Authors: Adekomi D.A. , Adewole O.S. , Dare B.J. , Olaniyan O.O. , Ojebisi R.M. , Ajao M.S.
Published: April 2021

Recently, Pentazocine and alcohol have become one of the drugs abused in the developing countries. However, there is the dearth of information on the effects of these substances on the cytological profile of the medial prefrontal cortex (mPFC) and cerebellar cortex; hence this study was aimed at evaluating the effect of these substances on the cytoarchitectural profile of the mPFC and cerebellar cortex of juvenile male rats. Twenty-four juvenile male rats were used for this study. They were randomly assigned to control (A), Alcohol-treated (B), Pentazocine-treated (C), and Pentazocine-Alcohol-treated group (D). Exposure to the various treatment paradigm was done s.c. twice daily (6hrs interval) for 14 days. It was observed that the cytological profile of the mPFC of the rats in the control groups was visible and well defined. In the B, C and D groups, there were numerous forms of neurodegeneration. There was also an increase in the density of astrocytes with the presence of glial scars. Furthermore, features of degenerative changes were also seen in the cerebellar cortex of the rats in the B, C, and D groups. It was observed from this study that exposure to Pentazocine-Alcohol combination triggers inflammatory and neurodegenerative processes in the cytoarchitectural profile of the mPFC and cerebellar cortex in juvenile male rats. These features could impair the functional integrity associated with these brain regions.Keywords: addiction, substance abuse, opioid, male, young
Dermatoglyphic patterns in type 2 diabetes mellitus
Authors: Sehmi S
Published: April 2021

Dermatoglyphics is the study of epidermal ridge patterns on the palm, fingers, sole and toes. They are genetically determined and are useful in diagnosis of various genetic diseases like Diabetes mellitus. There are three types of fingerprint patterns: Arches, loops and whorls. Finger prints of both hands are not same and they persist throughout life unless there is damage to the dermis. The patterns of fingertip patterns begin to form around the 13th week of intrauterine life. These patterns are inherited in from genetic makeup of the parents but the inheritance is not purely Mendelian. They get modified during the first few weeks of the intrauterine life due to pressure on the finger pads and on the palms.Keywords: Epidermal, Dermatoglyphics, Genetic, Arches
CCR5 HIV-1 receptor predominance in adult male prepuce; the major entry route for HIV-1 in indigenous black males in zambia?
Authors: Mukape Mukape , Kafumukache Elliot Bufuku , Trevor Kaile
Published: April 2021

HIV-1 entry requires not only CD4 molecule but also CCR5 (CD 195) and CXCR4 (CD 184) coreceptors. A number of randomised controlled trials in Africa have reported that male circumcision (MC) reduces the risk of HIV-1 acquisition by up to 60%. Other studies have reported that sexually transmitted infections (STIs) increase the risk of infection by HIV via an inflammatory  recruitment of more HIV target cells to the foreskin. Our aim was to compare the density of HIV-1 co-receptors (CCR5 and CXCR4) in naïve penile prepuce of neonates and penile prepuce of adults with and without history of ulcerative STIs at Male Circumcision Centres in Lusaka, Zambia. Twenty (20) fresh foreskin samples were included: five (5) from neonates, ten (10) from adult males without history of ulcerative STIs and five (5) from adult males with a history of ulcerative STIs. Immediately following MC, fresh foreskin specimens were fixed using 10% normal buffered formalin and transported to University Teaching Hospital (UTH) where tissues were processed and stained with anti-CD 195 and anti-CD 184 antibodies. Neonatal penile foreskin co-receptor mean density for CCR5 and CXCR4 was  13±5.148/mm2 and 7±1.581/mm2 respectively. CCR5 mean density of adults without past history of ulcerative STIs was 42.1±11.874/mm2 while those with history of ulcerative STIs was 78.6±13.520/mm2. Densities of CCR5 were all statistically  significant with both having Pvalue of 0.000. CXCR4 mean density was 18.6±4.812/mm2 in adults without past history ofulcerative STIs and 23.4±4.393/mm2 in those with history of ulcerative STIs giving an insignificant P-value of 0.084. It could be concluded that CCR5 co-receptors provide major entry route for HIV-1 in male adults and that CCR5 seemed to be mobilized more than CXCR4 to the prepuce during inflammation. This supports evidence that MC reduces CCR5 co-receptors for acquisition and transmission of R5 strains of HIV-1.Key words: HIV, CCR5, CD 195, CD 184.
Preventive and regenerative responses to 1- isothiocyanato-4-(methylsulfinyl) butane in annular puncture – induced model of rabbit intervertebral disc degeneration
Authors: Ogunlade B. , Adelakun S.A. , Fidelis O.P. , Owolabi F.M.
Published: April 2021

This study aimed at investigating the preventive and rejuvenating capacity of 1-isothiocyanato-4-(methylsulfinyl) butanein annular punctured intervertebral disc degeneration in rabbit model.Twentyfive New Zealand white rabbits (1.5 to 3.0 kg each) underwent annular puncture.Group 1 received 50 mg/kg of phosphate-buffered saline orally for 8 weeks (non-punctured group); group 2 received 50 mg/kg phosphate-buffered salinesolution immediately after puncture for 8 weeks (model saline group I); group 3 received 50 mg/kg phosphate-buffered salinesolution after 8 weeks of puncture for another 8 weeks (model saline group II); group 4 received the oral  administration of 450 mg/kg of 1-isothiocyanato-4- (methylsulfinyl) Butane solution immediately after puncture for 8 weeks; group 5 received the oral administration of 450 mg/kg of 1-isothiocyanato-4-(methylsulfinyl) Butane solution after 8 weeks of puncture for another 8 weeks.Serial X-rays were performed at 0, 8 and 16weeks for the disc height. Upon sacrifice, the whole spinal column and discs were extracted and analyzed for various histological staining techniques (H&E and HVG), biochemical and immunohistochemical analysis. There wasdecreased in disc height in the X- ray and was significantly prevented and regenerated after the administration of 1-isothiocyanato-4-(methylsulfinyl) Butane solution. The histological grade, collagen type 1 and 2, aggrecan, and matrix metalloprotease-13 mRNA expression and histological analysiswere definitive in the degeneration processes thereby affirming the X-ray data. This study therefore ascertain that 1-isothiocyanato-4-(methylsulfinyl) Butane had anabolic effects on degenerated disc and could provide valuable information for consideration in clinical trials.Keywords: Low back pain, intervertebral disc degeneration, 1-isothiocyanato-4-(methylsulfinyl) Butane,histology.
Morphometric study of the neurons in human hypoglossal nerve nucleus during early gestation
Authors: Sreeja M.T. , Vatsalaswamy P , Leo Rathinaraj A.S.
Published: April 2021

Hypoglossal nerve, XII cranial nerve is responsible for the motor innervation of the tongue muscles, which assists in various motor activities such as chewing, swallowing, vocalization. Thus, weakness of this nerve will lead to weakness and deviation of the tongue to one side. Clinically besides these functions it is also responsible for the most important function such as modulation of respiration and drinking behavior. Thus, a detailed study about the cell dynamics, which involves the development of neurons in the hypoglossal nerve nucleus becomes essential. 12 foetuses [Gestational age 10 – 24 weeks] were included in the study. They were divided into 4 groups based on their gestational age and CRL measurements. Hypoglossal nucleus extends throughout the length of medulla oblongata in the para-median plane. Tissues were collected from hind brain section and section of medulla. The tissues will be processed by routine histological procedure were stained with hematoxylin & eosin and also with Holmer’s Silver nitrate to study the histological details. Morphometric study covered the cell dimensions and volumes of hypoglossal neurons and its nucleus. From these data, coefficients were drawn to identify the proportion of growth between cell and nuclear volume. Morphometric analysis of hypoglossal nerve neurons in human from 10th to 24th gestational week concludes that the primitive migratory cells seen in the initial period and later it will become round neuroblast. In the initial 16 weeks nucleus occupied the entire volume of the cell.Keywords: Hypoglossal nerve nucleus, Deglutition, Mastication, Morphometry, Histogenesis